doc_id	sent_index	relation_id	relation	trigger	trigger_offset	arg_num	arg_head	arg_protein	arg_domain	arg_site	arg_sugar	arg_head_offset	arg_base_np	arg_base_np_offset	arg_np	arg_np_offset	sent_text
34211273	10	0	theme	tissue	1733:1738	arg1	engineering					1740:1750	bone tissue engineering	1728:1750	bone tissue engineering application after in vivo studies	1728:1784	This potential composite could be used as scaffolds in bone tissue engineering application after in vivo studies.
34211273	7	1	theme	composite	1130:1138	arg1	electrospun					1140:1150	The composite electrospun	1126:1150	The composite electrospun containing Chi/NNPs	1126:1170	The composite electrospun containing Chi/NNPs can form a double protective barrier which can effectively prolong the release time of NELL-1 growth factor.
34211273	5	2	theme	PCL/BNPs	894:901	arg1	group					903:907	PCL/BNPs group	894:907	PCL/BNPs group	894:907	RESULTS With the addition of nHA and nanoparticles, the fiber diameter of PCL/BNPs group, PCL/NNPs group and PCL/nHA/NNPs group was significantly increased.
34211273	10	3	used	used	1707:1710	arg2	composite					1688:1696	This potential composite	1673:1696	This potential composite	1673:1696	This potential composite could be used as scaffolds in bone tissue engineering application after in vivo studies.
34211273	10	3	used	used	1707:1710	arg2	scaffolds					1715:1723	scaffolds	1715:1723	scaffolds in bone tissue engineering application after in vivo studies	1715:1784	This potential composite could be used as scaffolds in bone tissue engineering application after in vivo studies.
34211273	1	4	theme	potential	254:262	arg1	systems					264:270	potential systems	254:270	potential systems	254:270	BACKGROUND As commonly bone defect is a disease of jaw that can seriously affect implant restoration, the bioactive scaffold can be used as potential systems to provide effective repair for bone defect.
34211273	5	5	theme	PCL/NNPs	910:917	arg1	group					919:923	PCL/NNPs group	910:923	PCL/NNPs group	910:923	RESULTS With the addition of nHA and nanoparticles, the fiber diameter of PCL/BNPs group, PCL/NNPs group and PCL/nHA/NNPs group was significantly increased.
34211273	10	6	from	scaffolds	1715:1723	arg1	application					1752:1762	bone tissue engineering application	1728:1762	bone tissue engineering application after in vivo studies	1728:1784	This potential composite could be used as scaffolds in bone tissue engineering application after in vivo studies.
34211273	3	7	from	nanoparticles	505:517	arg1	study					491:495	this study	486:495	this study	486:495	METHODS In this study, NELL-1 nanoparticles (Chi/NNP) and nano hydroxyapatite were incorporated in composite scaffolds by electrospinning and characterized using TEM, SEM, contact angle, tensile tests and in vitro drug release.
34211273	4	8	theme	osteogenic	786:795	arg1	activity					797:804	osteogenic activity	786:804	osteogenic activity	786:804	In vitro biological activities such as MC3T3-E1 cell attachment, proliferation and osteogenic activity were studied.
34211273	3	9	from	hydroxyapatite	538:551	arg1	study					491:495	this study	486:495	this study	486:495	METHODS In this study, NELL-1 nanoparticles (Chi/NNP) and nano hydroxyapatite were incorporated in composite scaffolds by electrospinning and characterized using TEM, SEM, contact angle, tensile tests and in vitro drug release.
34211273	6	10	theme	hydroxyl	1003:1010	arg1	group					1012:1016	the hydrophilic hydroxyl group	987:1016	the hydrophilic hydroxyl group	987:1016	Moreover, the hydrophilic hydroxyl group and amino group presented in nHA and nanoparticles had improved the hydrophilicity of the composite fibers.
34211273	3	11	theme	drug	689:692	arg1	release					694:700	in vitro drug release	680:700	in vitro drug release	680:700	METHODS In this study, NELL-1 nanoparticles (Chi/NNP) and nano hydroxyapatite were incorporated in composite scaffolds by electrospinning and characterized using TEM, SEM, contact angle, tensile tests and in vitro drug release.
34211273	7	12	contain	containing	1152:1161	arg2	Chi/NNPs					1163:1170	Chi/NNPs	1163:1170	Chi/NNPs	1163:1170	The composite electrospun containing Chi/NNPs can form a double protective barrier which can effectively prolong the release time of NELL-1 growth factor.
34211273	7	12	contain	containing	1152:1161	arg1	electrospun					1140:1150	The composite electrospun	1126:1150	The composite electrospun containing Chi/NNPs	1126:1170	The composite electrospun containing Chi/NNPs can form a double protective barrier which can effectively prolong the release time of NELL-1 growth factor.
34211273	4	13	theme	MC3T3-E1	742:749	arg1	cell					751:754	MC3T3-E1 cell attachment, proliferation and osteogenic activity	742:804	cell	751:754	In vitro biological activities such as MC3T3-E1 cell attachment, proliferation and osteogenic activity were studied.
34211273	6	14	theme	hydrophilic	991:1001	arg1	group					1012:1016	the hydrophilic hydroxyl group	987:1016	the hydrophilic hydroxyl group	987:1016	Moreover, the hydrophilic hydroxyl group and amino group presented in nHA and nanoparticles had improved the hydrophilicity of the composite fibers.
34211273	5	15	theme	PCL/nHA/NNPs	929:940	arg1	group					942:946	PCL/nHA/NNPs group	929:946	PCL/nHA/NNPs group	929:946	RESULTS With the addition of nHA and nanoparticles, the fiber diameter of PCL/BNPs group, PCL/NNPs group and PCL/nHA/NNPs group was significantly increased.
34211273	7	16	theme	growth	1266:1271	arg1	factor					1273:1278	NELL-1 growth factor	1259:1278	NELL-1 growth factor	1259:1278	The composite electrospun containing Chi/NNPs can form a double protective barrier which can effectively prolong the release time of NELL-1 growth factor.
34211273	7	17	theme	NELL-1	1259:1264	arg1	factor					1273:1278	NELL-1 growth factor	1259:1278	NELL-1 growth factor	1259:1278	The composite electrospun containing Chi/NNPs can form a double protective barrier which can effectively prolong the release time of NELL-1 growth factor.
34211273	2	18	theme	osteoinductive	327:340	arg1	scaffold					366:373	A osteoinductive bone tissue engineering scaffold	325:373	PURPOSE A osteoinductive bone tissue engineering scaffold	317:373	PURPOSE A osteoinductive bone tissue engineering scaffold has been prepared in order to explore the effect of bioactive materials on bone tissue engineering.
34211273	4	19	dep	cell	751:754	arg1	attachment					756:765	attachment	756:765	attachment	756:765	In vitro biological activities such as MC3T3-E1 cell attachment, proliferation and osteogenic activity were studied.
34211273	7	20	theme	double	1183:1188	arg1	barrier					1201:1207	a double protective barrier	1181:1207	a double protective barrier which can effectively prolong the release time of NELL-1 growth factor	1181:1278	The composite electrospun containing Chi/NNPs can form a double protective barrier which can effectively prolong the release time of NELL-1 growth factor.
34211273	9	21	theme	PCL/nHA/NNPs	1550:1561	arg1	fiber					1573:1577	A multi-functional PCL/nHA/NNPs composite fiber	1531:1577	A multi-functional PCL/nHA/NNPs composite fiber with long-term bioactivity and osteoinductivity	1531:1625	CONCLUSION A multi-functional PCL/nHA/NNPs composite fiber with long-term bioactivity and osteoinductivity was successfully prepared by electrospinning.
34211273	10	22	theme	in	1770:1771	arg1	studies					1778:1784	in vivo studies	1770:1784	in vivo studies	1770:1784	This potential composite could be used as scaffolds in bone tissue engineering application after in vivo studies.
34211273	5	23	theme	nanoparticles	857:869	arg1	addition					837:844	the addition	833:844	the addition of nHA and nanoparticles, the fiber diameter of PCL/BNPs group, PCL/NNPs group and PCL/nHA/NNPs group	833:946	RESULTS With the addition of nHA and nanoparticles, the fiber diameter of PCL/BNPs group, PCL/NNPs group and PCL/nHA/NNPs group was significantly increased.
34211273	3	24	dep	in	680:681	arg1	vitro					683:687	vitro	683:687	vitro	683:687	METHODS In this study, NELL-1 nanoparticles (Chi/NNP) and nano hydroxyapatite were incorporated in composite scaffolds by electrospinning and characterized using TEM, SEM, contact angle, tensile tests and in vitro drug release.
34211273	5	25	theme	group	903:907	arg1	nHA					849:851	nHA	849:851	nHA	849:851	RESULTS With the addition of nHA and nanoparticles, the fiber diameter of PCL/BNPs group, PCL/NNPs group and PCL/nHA/NNPs group was significantly increased.
34211273	5	25	theme	group	903:907	arg1	diameter					882:889	the fiber diameter	872:889	the fiber diameter of PCL/BNPs group, PCL/NNPs group and PCL/nHA/NNPs group	872:946	RESULTS With the addition of nHA and nanoparticles, the fiber diameter of PCL/BNPs group, PCL/NNPs group and PCL/nHA/NNPs group was significantly increased.
34211273	3	26	theme	nano	533:536	arg1	hydroxyapatite					538:551	nano hydroxyapatite	533:551	nano hydroxyapatite	533:551	METHODS In this study, NELL-1 nanoparticles (Chi/NNP) and nano hydroxyapatite were incorporated in composite scaffolds by electrospinning and characterized using TEM, SEM, contact angle, tensile tests and in vitro drug release.
34211273	2	27	theme	tissue	455:460	arg1	engineering					462:472	bone tissue engineering	450:472	bone tissue engineering	450:472	PURPOSE A osteoinductive bone tissue engineering scaffold has been prepared in order to explore the effect of bioactive materials on bone tissue engineering.
34211273	7	28	theme	release	1243:1249	arg1	time					1251:1254	the release time	1239:1254	the release time of NELL-1 growth factor	1239:1278	The composite electrospun containing Chi/NNPs can form a double protective barrier which can effectively prolong the release time of NELL-1 growth factor.
34211273	3	29	theme	composite	574:582	arg1	scaffolds					584:592	composite scaffolds	574:592	composite scaffolds	574:592	METHODS In this study, NELL-1 nanoparticles (Chi/NNP) and nano hydroxyapatite were incorporated in composite scaffolds by electrospinning and characterized using TEM, SEM, contact angle, tensile tests and in vitro drug release.
34211273	7	30	theme	protective	1190:1199	arg1	barrier					1201:1207	a double protective barrier	1181:1207	a double protective barrier which can effectively prolong the release time of NELL-1 growth factor	1181:1278	The composite electrospun containing Chi/NNPs can form a double protective barrier which can effectively prolong the release time of NELL-1 growth factor.
34211273	1	31	theme	implant	195:201	arg1	restoration					203:213	implant restoration	195:213	implant restoration	195:213	BACKGROUND As commonly bone defect is a disease of jaw that can seriously affect implant restoration, the bioactive scaffold can be used as potential systems to provide effective repair for bone defect.
34211273	1	31	theme	implant	195:201	arg1	scaffold					230:237	the bioactive scaffold	216:237	the bioactive scaffold can be used as potential systems to provide effective repair for bone defect	216:314	BACKGROUND As commonly bone defect is a disease of jaw that can seriously affect implant restoration, the bioactive scaffold can be used as potential systems to provide effective repair for bone defect.
34211273	2	32	theme	bone	450:453	arg1	engineering					462:472	bone tissue engineering	450:472	bone tissue engineering	450:472	PURPOSE A osteoinductive bone tissue engineering scaffold has been prepared in order to explore the effect of bioactive materials on bone tissue engineering.
34211273	1	33	theme	effective	283:291	arg1	repair					293:298	effective repair	283:298	effective repair	283:298	BACKGROUND As commonly bone defect is a disease of jaw that can seriously affect implant restoration, the bioactive scaffold can be used as potential systems to provide effective repair for bone defect.
34211273	4	34	theme	biological	712:721	arg1	cell					751:754	MC3T3-E1 cell attachment, proliferation and osteogenic activity	742:804	cell	751:754	In vitro biological activities such as MC3T3-E1 cell attachment, proliferation and osteogenic activity were studied.
34211273	4	34	theme	biological	712:721	arg1	proliferation					768:780	proliferation	768:780	proliferation	768:780	In vitro biological activities such as MC3T3-E1 cell attachment, proliferation and osteogenic activity were studied.
34211273	4	34	theme	biological	712:721	arg1	activity					797:804	osteogenic activity	786:804	osteogenic activity	786:804	In vitro biological activities such as MC3T3-E1 cell attachment, proliferation and osteogenic activity were studied.
34211273	4	34	theme	biological	712:721	arg1	activities					723:732	In vitro biological activities	703:732	In vitro biological activities such as MC3T3-E1 cell attachment, proliferation and osteogenic activity	703:804	In vitro biological activities such as MC3T3-E1 cell attachment, proliferation and osteogenic activity were studied.
34211273	5	35	theme	group	942:946	arg1	nHA					849:851	nHA	849:851	nHA	849:851	RESULTS With the addition of nHA and nanoparticles, the fiber diameter of PCL/BNPs group, PCL/NNPs group and PCL/nHA/NNPs group was significantly increased.
34211273	5	35	theme	group	942:946	arg1	diameter					882:889	the fiber diameter	872:889	the fiber diameter of PCL/BNPs group, PCL/NNPs group and PCL/nHA/NNPs group	872:946	RESULTS With the addition of nHA and nanoparticles, the fiber diameter of PCL/BNPs group, PCL/NNPs group and PCL/nHA/NNPs group was significantly increased.
34211273	4	36	dep	In	703:704	arg1	vitro					706:710	vitro	706:710	vitro	706:710	In vitro biological activities such as MC3T3-E1 cell attachment, proliferation and osteogenic activity were studied.
34211273	5	37	theme	fiber	876:880	arg1	nHA					849:851	nHA	849:851	nHA	849:851	RESULTS With the addition of nHA and nanoparticles, the fiber diameter of PCL/BNPs group, PCL/NNPs group and PCL/nHA/NNPs group was significantly increased.
34211273	5	37	theme	fiber	876:880	arg1	diameter					882:889	the fiber diameter	872:889	the fiber diameter of PCL/BNPs group, PCL/NNPs group and PCL/nHA/NNPs group	872:946	RESULTS With the addition of nHA and nanoparticles, the fiber diameter of PCL/BNPs group, PCL/NNPs group and PCL/nHA/NNPs group was significantly increased.
34211273	5	38	with	RESULTS	820:826	arg1	addition					837:844	the addition	833:844	the addition of nHA and nanoparticles, the fiber diameter of PCL/BNPs group, PCL/NNPs group and PCL/nHA/NNPs group	833:946	RESULTS With the addition of nHA and nanoparticles, the fiber diameter of PCL/BNPs group, PCL/NNPs group and PCL/nHA/NNPs group was significantly increased.
34211273	8	39	theme	good	1430:1433	arg1	cytocompatibility					1435:1451	good cytocompatibility	1430:1451	good cytocompatibility	1430:1451	In addition, the hydroxyapatite/NELL-1 nanoparticles electrospun fibers can promote attachment, proliferation, differentiation of MC3T3-E1 cells and good cytocompatibility, indicating better ability of inducing osteogenic differentiation.
34211273	2	40	theme	bioactive	427:435	arg1	materials					437:445	bioactive materials	427:445	bioactive materials	427:445	PURPOSE A osteoinductive bone tissue engineering scaffold has been prepared in order to explore the effect of bioactive materials on bone tissue engineering.
34211273	2	41	theme	materials	437:445	arg1	effect					417:422	the effect	413:422	the effect of bioactive materials on bone tissue engineering	413:472	PURPOSE A osteoinductive bone tissue engineering scaffold has been prepared in order to explore the effect of bioactive materials on bone tissue engineering.
34211273	9	42	theme	composite	1563:1571	arg1	fiber					1573:1577	A multi-functional PCL/nHA/NNPs composite fiber	1531:1577	A multi-functional PCL/nHA/NNPs composite fiber with long-term bioactivity and osteoinductivity	1531:1625	CONCLUSION A multi-functional PCL/nHA/NNPs composite fiber with long-term bioactivity and osteoinductivity was successfully prepared by electrospinning.
34211273	9	43	with	fiber	1573:1577	arg1	bioactivity					1594:1604	long-term bioactivity	1584:1604	long-term bioactivity	1584:1604	CONCLUSION A multi-functional PCL/nHA/NNPs composite fiber with long-term bioactivity and osteoinductivity was successfully prepared by electrospinning.
34211273	9	43	with	fiber	1573:1577	arg1	osteoinductivity					1610:1625	osteoinductivity	1610:1625	osteoinductivity	1610:1625	CONCLUSION A multi-functional PCL/nHA/NNPs composite fiber with long-term bioactivity and osteoinductivity was successfully prepared by electrospinning.
34211273	1	44	theme	bone	137:140	arg1	defect					142:147	bone defect	137:147	BACKGROUND As commonly bone defect	114:147	BACKGROUND As commonly bone defect is a disease of jaw that can seriously affect implant restoration, the bioactive scaffold can be used as potential systems to provide effective repair for bone defect.
34211273	1	44	theme	bone	137:140	arg1	disease					154:160	a disease	152:160	a disease of jaw that can seriously affect implant restoration, the bioactive scaffold can be used as potential systems to provide effective repair for bone defect	152:314	BACKGROUND As commonly bone defect is a disease of jaw that can seriously affect implant restoration, the bioactive scaffold can be used as potential systems to provide effective repair for bone defect.
34211273	1	44	theme	bone	137:140	arg1	BACKGROUND					114:123	BACKGROUND	114:123	BACKGROUND As commonly bone defect	114:147	BACKGROUND As commonly bone defect is a disease of jaw that can seriously affect implant restoration, the bioactive scaffold can be used as potential systems to provide effective repair for bone defect.
34211273	7	45	theme	factor	1273:1278	arg1	time					1251:1254	the release time	1239:1254	the release time of NELL-1 growth factor	1239:1278	The composite electrospun containing Chi/NNPs can form a double protective barrier which can effectively prolong the release time of NELL-1 growth factor.
34211273	10	46	theme	potential	1678:1686	arg1	scaffolds					1715:1723	scaffolds	1715:1723	scaffolds in bone tissue engineering application after in vivo studies	1715:1784	This potential composite could be used as scaffolds in bone tissue engineering application after in vivo studies.
34211273	10	46	theme	potential	1678:1686	arg1	composite					1688:1696	This potential composite	1673:1696	This potential composite	1673:1696	This potential composite could be used as scaffolds in bone tissue engineering application after in vivo studies.
34211273	1	47	theme	bone	304:307	arg1	defect					309:314	bone defect	304:314	bone defect	304:314	BACKGROUND As commonly bone defect is a disease of jaw that can seriously affect implant restoration, the bioactive scaffold can be used as potential systems to provide effective repair for bone defect.
34211273	9	48	theme	long-term	1584:1592	arg1	bioactivity					1594:1604	long-term bioactivity	1584:1604	long-term bioactivity	1584:1604	CONCLUSION A multi-functional PCL/nHA/NNPs composite fiber with long-term bioactivity and osteoinductivity was successfully prepared by electrospinning.
34211273	5	49	theme	nHA	849:851	arg1	addition					837:844	the addition	833:844	the addition of nHA and nanoparticles, the fiber diameter of PCL/BNPs group, PCL/NNPs group and PCL/nHA/NNPs group	833:946	RESULTS With the addition of nHA and nanoparticles, the fiber diameter of PCL/BNPs group, PCL/NNPs group and PCL/nHA/NNPs group was significantly increased.
34211273	9	50	dep	CONCLUSION	1520:1529	arg1	prepared					1644:1651	prepared	1644:1651	was successfully prepared by electrospinning	1627:1670	CONCLUSION A multi-functional PCL/nHA/NNPs composite fiber with long-term bioactivity and osteoinductivity was successfully prepared by electrospinning.
34211273	1	51	theme	bioactive	220:228	arg1	restoration					203:213	implant restoration	195:213	implant restoration	195:213	BACKGROUND As commonly bone defect is a disease of jaw that can seriously affect implant restoration, the bioactive scaffold can be used as potential systems to provide effective repair for bone defect.
34211273	1	51	theme	bioactive	220:228	arg1	scaffold					230:237	the bioactive scaffold	216:237	the bioactive scaffold can be used as potential systems to provide effective repair for bone defect	216:314	BACKGROUND As commonly bone defect is a disease of jaw that can seriously affect implant restoration, the bioactive scaffold can be used as potential systems to provide effective repair for bone defect.
34211273	10	52	theme	engineering	1740:1750	arg1	application					1752:1762	bone tissue engineering application	1728:1762	bone tissue engineering application after in vivo studies	1728:1784	This potential composite could be used as scaffolds in bone tissue engineering application after in vivo studies.
34211273	8	53	theme	electrospun	1334:1344	arg1	fibers					1346:1351	electrospun fibers	1334:1351	electrospun fibers	1334:1351	In addition, the hydroxyapatite/NELL-1 nanoparticles electrospun fibers can promote attachment, proliferation, differentiation of MC3T3-E1 cells and good cytocompatibility, indicating better ability of inducing osteogenic differentiation.
34211273	0	54	theme	Bone	77:80	arg1	Application					101:111	Bone Tissue Engineering Application	77:111	Bone Tissue Engineering Application	77:111	Hydroxyapatite/NELL-1 Nanoparticles Electrospun Fibers for Osteoinduction in Bone Tissue Engineering Application.
34211273	8	55	theme	cells	1420:1424	arg1	differentiation					1392:1406	differentiation	1392:1406	differentiation of MC3T3-E1 cells	1392:1424	In addition, the hydroxyapatite/NELL-1 nanoparticles electrospun fibers can promote attachment, proliferation, differentiation of MC3T3-E1 cells and good cytocompatibility, indicating better ability of inducing osteogenic differentiation.
34211273	8	55	theme	cells	1420:1424	arg1	proliferation					1377:1389	proliferation	1377:1389	proliferation	1377:1389	In addition, the hydroxyapatite/NELL-1 nanoparticles electrospun fibers can promote attachment, proliferation, differentiation of MC3T3-E1 cells and good cytocompatibility, indicating better ability of inducing osteogenic differentiation.
34211273	8	55	theme	cells	1420:1424	arg1	attachment					1365:1374	attachment	1365:1374	attachment	1365:1374	In addition, the hydroxyapatite/NELL-1 nanoparticles electrospun fibers can promote attachment, proliferation, differentiation of MC3T3-E1 cells and good cytocompatibility, indicating better ability of inducing osteogenic differentiation.
34211273	8	55	theme	cells	1420:1424	arg1	cytocompatibility					1435:1451	good cytocompatibility	1430:1451	good cytocompatibility	1430:1451	In addition, the hydroxyapatite/NELL-1 nanoparticles electrospun fibers can promote attachment, proliferation, differentiation of MC3T3-E1 cells and good cytocompatibility, indicating better ability of inducing osteogenic differentiation.
34211273	3	56	theme	contact	647:653	arg1	angle					655:659	contact angle	647:659	contact angle	647:659	METHODS In this study, NELL-1 nanoparticles (Chi/NNP) and nano hydroxyapatite were incorporated in composite scaffolds by electrospinning and characterized using TEM, SEM, contact angle, tensile tests and in vitro drug release.
34211273	8	57	theme	better	1465:1470	arg1	ability					1472:1478	better ability	1465:1478	better ability of inducing osteogenic differentiation	1465:1517	In addition, the hydroxyapatite/NELL-1 nanoparticles electrospun fibers can promote attachment, proliferation, differentiation of MC3T3-E1 cells and good cytocompatibility, indicating better ability of inducing osteogenic differentiation.
34211273	8	58	theme	MC3T3-E1	1411:1418	arg1	cells					1420:1424	MC3T3-E1 cells	1411:1424	MC3T3-E1 cells	1411:1424	In addition, the hydroxyapatite/NELL-1 nanoparticles electrospun fibers can promote attachment, proliferation, differentiation of MC3T3-E1 cells and good cytocompatibility, indicating better ability of inducing osteogenic differentiation.
34211273	2	59	theme	engineering	354:364	arg1	scaffold					366:373	A osteoinductive bone tissue engineering scaffold	325:373	PURPOSE A osteoinductive bone tissue engineering scaffold	317:373	PURPOSE A osteoinductive bone tissue engineering scaffold has been prepared in order to explore the effect of bioactive materials on bone tissue engineering.
34211273	10	60	theme	bone	1728:1731	arg1	engineering					1740:1750	bone tissue engineering	1728:1750	bone tissue engineering application after in vivo studies	1728:1784	This potential composite could be used as scaffolds in bone tissue engineering application after in vivo studies.
34211273	3	61	from	METHODS	475:481	arg1	study					491:495	this study	486:495	this study	486:495	METHODS In this study, NELL-1 nanoparticles (Chi/NNP) and nano hydroxyapatite were incorporated in composite scaffolds by electrospinning and characterized using TEM, SEM, contact angle, tensile tests and in vitro drug release.
34211273	2	62	theme	tissue	347:352	arg1	scaffold					366:373	A osteoinductive bone tissue engineering scaffold	325:373	PURPOSE A osteoinductive bone tissue engineering scaffold	317:373	PURPOSE A osteoinductive bone tissue engineering scaffold has been prepared in order to explore the effect of bioactive materials on bone tissue engineering.
34211273	3	63	theme	in	680:681	arg1	release					694:700	in vitro drug release	680:700	in vitro drug release	680:700	METHODS In this study, NELL-1 nanoparticles (Chi/NNP) and nano hydroxyapatite were incorporated in composite scaffolds by electrospinning and characterized using TEM, SEM, contact angle, tensile tests and in vitro drug release.
34211273	10	64	dep	in	1770:1771	arg1	vivo					1773:1776	vivo	1773:1776	vivo	1773:1776	This potential composite could be used as scaffolds in bone tissue engineering application after in vivo studies.
34211273	2	65	theme	bone	342:345	arg1	scaffold					366:373	A osteoinductive bone tissue engineering scaffold	325:373	PURPOSE A osteoinductive bone tissue engineering scaffold	317:373	PURPOSE A osteoinductive bone tissue engineering scaffold has been prepared in order to explore the effect of bioactive materials on bone tissue engineering.
34211273	3	66	theme	tensile	662:668	arg1	tests					670:674	tensile tests	662:674	tensile tests	662:674	METHODS In this study, NELL-1 nanoparticles (Chi/NNP) and nano hydroxyapatite were incorporated in composite scaffolds by electrospinning and characterized using TEM, SEM, contact angle, tensile tests and in vitro drug release.
34211273	1	67	dep	scaffold	230:237	arg1	used					246:249	used	246:249	can be used as potential systems to provide effective repair for bone defect	239:314	BACKGROUND As commonly bone defect is a disease of jaw that can seriously affect implant restoration, the bioactive scaffold can be used as potential systems to provide effective repair for bone defect.
34211273	2	68	from	effect	417:422	arg1	engineering					462:472	bone tissue engineering	450:472	bone tissue engineering	450:472	PURPOSE A osteoinductive bone tissue engineering scaffold has been prepared in order to explore the effect of bioactive materials on bone tissue engineering.
34211273	2	69	dep	PURPOSE	317:323	arg1	scaffold					366:373	A osteoinductive bone tissue engineering scaffold	325:373	PURPOSE A osteoinductive bone tissue engineering scaffold	317:373	PURPOSE A osteoinductive bone tissue engineering scaffold has been prepared in order to explore the effect of bioactive materials on bone tissue engineering.
34211273	5	70	theme	group	919:923	arg1	nHA					849:851	nHA	849:851	nHA	849:851	RESULTS With the addition of nHA and nanoparticles, the fiber diameter of PCL/BNPs group, PCL/NNPs group and PCL/nHA/NNPs group was significantly increased.
34211273	5	70	theme	group	919:923	arg1	diameter					882:889	the fiber diameter	872:889	the fiber diameter of PCL/BNPs group, PCL/NNPs group and PCL/nHA/NNPs group	872:946	RESULTS With the addition of nHA and nanoparticles, the fiber diameter of PCL/BNPs group, PCL/NNPs group and PCL/nHA/NNPs group was significantly increased.
34211273	0	71	theme	Engineering	89:99	arg1	Application					101:111	Bone Tissue Engineering Application	77:111	Bone Tissue Engineering Application	77:111	Hydroxyapatite/NELL-1 Nanoparticles Electrospun Fibers for Osteoinduction in Bone Tissue Engineering Application.
34211273	6	72	theme	composite	1108:1116	arg1	fibers					1118:1123	the composite fibers	1104:1123	the composite fibers	1104:1123	Moreover, the hydrophilic hydroxyl group and amino group presented in nHA and nanoparticles had improved the hydrophilicity of the composite fibers.
34211273	8	73	theme	osteogenic	1492:1501	arg1	differentiation					1503:1517	osteogenic differentiation	1492:1517	osteogenic differentiation	1492:1517	In addition, the hydroxyapatite/NELL-1 nanoparticles electrospun fibers can promote attachment, proliferation, differentiation of MC3T3-E1 cells and good cytocompatibility, indicating better ability of inducing osteogenic differentiation.
34211273	9	74	theme	multi-functional	1533:1548	arg1	fiber					1573:1577	A multi-functional PCL/nHA/NNPs composite fiber	1531:1577	A multi-functional PCL/nHA/NNPs composite fiber with long-term bioactivity and osteoinductivity	1531:1625	CONCLUSION A multi-functional PCL/nHA/NNPs composite fiber with long-term bioactivity and osteoinductivity was successfully prepared by electrospinning.
34211273	6	75	theme	fibers	1118:1123	arg1	hydrophilicity					1086:1099	the hydrophilicity	1082:1099	the hydrophilicity of the composite fibers	1082:1123	Moreover, the hydrophilic hydroxyl group and amino group presented in nHA and nanoparticles had improved the hydrophilicity of the composite fibers.
34211273	6	76	theme	amino	1022:1026	arg1	group					1028:1032	amino group	1022:1032	amino group	1022:1032	Moreover, the hydrophilic hydroxyl group and amino group presented in nHA and nanoparticles had improved the hydrophilicity of the composite fibers.
34211273	0	77	theme	Tissue	82:87	arg1	Application					101:111	Bone Tissue Engineering Application	77:111	Bone Tissue Engineering Application	77:111	Hydroxyapatite/NELL-1 Nanoparticles Electrospun Fibers for Osteoinduction in Bone Tissue Engineering Application.
34211273	0	78	theme	Electrospun	36:46	arg1	Fibers					48:53	Electrospun Fibers	36:53	Electrospun Fibers for Osteoinduction	36:72	Hydroxyapatite/NELL-1 Nanoparticles Electrospun Fibers for Osteoinduction in Bone Tissue Engineering Application.
34211273	1	79	theme	jaw	165:167	arg1	defect					142:147	bone defect	137:147	BACKGROUND As commonly bone defect	114:147	BACKGROUND As commonly bone defect is a disease of jaw that can seriously affect implant restoration, the bioactive scaffold can be used as potential systems to provide effective repair for bone defect.
34211273	1	79	theme	jaw	165:167	arg1	disease					154:160	a disease	152:160	a disease of jaw that can seriously affect implant restoration, the bioactive scaffold can be used as potential systems to provide effective repair for bone defect	152:314	BACKGROUND As commonly bone defect is a disease of jaw that can seriously affect implant restoration, the bioactive scaffold can be used as potential systems to provide effective repair for bone defect.
34211273	1	79	theme	jaw	165:167	arg1	BACKGROUND					114:123	BACKGROUND	114:123	BACKGROUND As commonly bone defect	114:147	BACKGROUND As commonly bone defect is a disease of jaw that can seriously affect implant restoration, the bioactive scaffold can be used as potential systems to provide effective repair for bone defect.
34211273	3	80	theme	NELL-1	498:503	arg1	Chi/NNP					520:526	Chi/NNP	520:526	Chi/NNP	520:526	METHODS In this study, NELL-1 nanoparticles (Chi/NNP) and nano hydroxyapatite were incorporated in composite scaffolds by electrospinning and characterized using TEM, SEM, contact angle, tensile tests and in vitro drug release.
34211273	3	80	theme	NELL-1	498:503	arg1	nanoparticles					505:517	NELL-1 nanoparticles	498:517	NELL-1 nanoparticles (Chi/NNP)	498:527	METHODS In this study, NELL-1 nanoparticles (Chi/NNP) and nano hydroxyapatite were incorporated in composite scaffolds by electrospinning and characterized using TEM, SEM, contact angle, tensile tests and in vitro drug release.
34211273	4	81	theme	In	703:704	arg1	cell					751:754	MC3T3-E1 cell attachment, proliferation and osteogenic activity	742:804	cell	751:754	In vitro biological activities such as MC3T3-E1 cell attachment, proliferation and osteogenic activity were studied.
34211273	4	81	theme	In	703:704	arg1	proliferation					768:780	proliferation	768:780	proliferation	768:780	In vitro biological activities such as MC3T3-E1 cell attachment, proliferation and osteogenic activity were studied.
34211273	4	81	theme	In	703:704	arg1	activity					797:804	osteogenic activity	786:804	osteogenic activity	786:804	In vitro biological activities such as MC3T3-E1 cell attachment, proliferation and osteogenic activity were studied.
34211273	4	81	theme	In	703:704	arg1	activities					723:732	In vitro biological activities	703:732	In vitro biological activities such as MC3T3-E1 cell attachment, proliferation and osteogenic activity	703:804	In vitro biological activities such as MC3T3-E1 cell attachment, proliferation and osteogenic activity were studied.
32673717	5	0	dep	825.97	955:960	arg1	VI					934:935	VI	934:935	VI	934:935	The maximum adsorption capacities of Cr(VI)/Pb(II) ions were 825.97 and 913.88 mg g-1, respectively.
32673717	4	1	theme	/Pb	754:756	arg1	ions					762:765	/Pb(II) ions	754:765	/Pb(II) ions removal	754:773	The adsorption kinetics for Cr(VI)/Pb(II) ions removal followed the pseudo-second order kinetic model and the equilibrium adsorption data were well fitted by Langmuir model.
32673717	2	2	theme	calcium	324:330	arg1	cross-linker					358:369	a cross-linker	356:369	a cross-linker for incorporation with Fe-TAC metal organic frameworks (MOFs) for the formation of PHM composite on in situ synthesis method	356:494	The pectin hydrogel (PH) was derived from mandarin orange peels by-products and calcium chloride was applied as a cross-linker for incorporation with Fe-TAC metal organic frameworks (MOFs) for the formation of PHM composite on in situ synthesis method.
32673717	2	2	theme	calcium	324:330	arg1	chloride					332:339	calcium chloride	324:339	calcium chloride	324:339	The pectin hydrogel (PH) was derived from mandarin orange peels by-products and calcium chloride was applied as a cross-linker for incorporation with Fe-TAC metal organic frameworks (MOFs) for the formation of PHM composite on in situ synthesis method.
32673717	1	3	theme	recent	230:235	arg1	years					237:241	recent years	230:241	recent years	230:241	The hydrogels-metal organic frameworks hybrid materials have received increasing attentions in recent years.
32673717	0	4	theme	composite	80:88	arg1	frameworks					69:78	metal-organic frameworks	55:78	metal-organic frameworks composite for enhanced Cr(VI) and Pb(II) ions removal	55:132	Novel derived pectin hydrogel from mandarin peel based metal-organic frameworks composite for enhanced Cr(VI) and Pb(II) ions removal.
32673717	3	5	theme	species	640:646	arg1	adsorption					618:627	the adsorption	614:627	the adsorption of anionic species, Cr(VI) as well as cationic species, Pb(II) ions from aqueous solution	614:717	The synthesized PHM composite was characterized by SEM, FT-IR, XRD and N2 adsorption-desorption and investigated for the adsorption of anionic species, Cr(VI) as well as cationic species, Pb(II) ions from aqueous solution.
32673717	7	6	dep	Cr	1278:1279	arg1	VI					1281:1282	VI	1281:1282	VI	1281:1282	The validity of PHM composite for simultaneous removal from real water matrices were confirmed as Cr(VI) (99.73, 99.87 and 99.87%) and Pb(II) (99.02, 98.55 and 98.55%) from tap water, sea water and industrial wastewater samples, respectively.
32673717	4	7	dep	followed	775:782	arg1	VI					751:752	VI	751:752	VI	751:752	The adsorption kinetics for Cr(VI)/Pb(II) ions removal followed the pseudo-second order kinetic model and the equilibrium adsorption data were well fitted by Langmuir model.
32673717	2	8	theme	synthesis	479:487	arg1	method					489:494	in situ synthesis method	471:494	in situ synthesis method	471:494	The pectin hydrogel (PH) was derived from mandarin orange peels by-products and calcium chloride was applied as a cross-linker for incorporation with Fe-TAC metal organic frameworks (MOFs) for the formation of PHM composite on in situ synthesis method.
32673717	7	9	theme	PHM	1196:1198	arg1	validity					1184:1191	The validity	1180:1191	The validity of PHM composite for simultaneous removal from real water matrices	1180:1258	The validity of PHM composite for simultaneous removal from real water matrices were confirmed as Cr(VI) (99.73, 99.87 and 99.87%) and Pb(II) (99.02, 98.55 and 98.55%) from tap water, sea water and industrial wastewater samples, respectively.
32673717	7	9	theme	PHM	1196:1198	arg1	Pb					1315:1316	Pb(II)	1315:1320	Pb(II) (99.02, 98.55 and 98.55%)	1315:1346	The validity of PHM composite for simultaneous removal from real water matrices were confirmed as Cr(VI) (99.73, 99.87 and 99.87%) and Pb(II) (99.02, 98.55 and 98.55%) from tap water, sea water and industrial wastewater samples, respectively.
32673717	7	9	theme	PHM	1196:1198	arg1	Cr					1278:1279	Cr	1278:1279	Cr(VI) (99.73, 99.87 and 99.87%)	1278:1309	The validity of PHM composite for simultaneous removal from real water matrices were confirmed as Cr(VI) (99.73, 99.87 and 99.87%) and Pb(II) (99.02, 98.55 and 98.55%) from tap water, sea water and industrial wastewater samples, respectively.
32673717	7	10	theme	composite	1200:1208	arg1	PHM					1196:1198	PHM	1196:1198	PHM composite for simultaneous removal from real water matrices	1196:1258	The validity of PHM composite for simultaneous removal from real water matrices were confirmed as Cr(VI) (99.73, 99.87 and 99.87%) and Pb(II) (99.02, 98.55 and 98.55%) from tap water, sea water and industrial wastewater samples, respectively.
32673717	6	11	theme	PHM	1055:1057	arg1	composite					1059:1067	PHM composite	1055:1067	PHM composite	1055:1067	High swelling capacity (1500.0%) was also characterized for PHM composite after only 400 min and excellent stability for eight cycles with respect to regeneration with 0.1 mol L-1 HCl.
32673717	3	12	from	adsorption	618:627	arg1	solution					710:717	aqueous solution	702:717	aqueous solution	702:717	The synthesized PHM composite was characterized by SEM, FT-IR, XRD and N2 adsorption-desorption and investigated for the adsorption of anionic species, Cr(VI) as well as cationic species, Pb(II) ions from aqueous solution.
32673717	0	13	theme	enhanced	94:101	arg1	Cr					103:104	enhanced Cr(VI)	94:108	enhanced Cr(VI)	94:108	Novel derived pectin hydrogel from mandarin peel based metal-organic frameworks composite for enhanced Cr(VI) and Pb(II) ions removal.
32673717	2	14	with	incorporation	375:387	arg1	frameworks					415:424	Fe-TAC metal organic frameworks	394:424	Fe-TAC metal organic frameworks (MOFs) for the formation of PHM composite on in situ synthesis method	394:494	The pectin hydrogel (PH) was derived from mandarin orange peels by-products and calcium chloride was applied as a cross-linker for incorporation with Fe-TAC metal organic frameworks (MOFs) for the formation of PHM composite on in situ synthesis method.
32673717	2	14	with	incorporation	375:387	arg1	MOFs					427:430	MOFs	427:430	MOFs	427:430	The pectin hydrogel (PH) was derived from mandarin orange peels by-products and calcium chloride was applied as a cross-linker for incorporation with Fe-TAC metal organic frameworks (MOFs) for the formation of PHM composite on in situ synthesis method.
32673717	7	15	theme	sea	1364:1366	arg1	water					1368:1372	sea water	1364:1372	sea water	1364:1372	The validity of PHM composite for simultaneous removal from real water matrices were confirmed as Cr(VI) (99.73, 99.87 and 99.87%) and Pb(II) (99.02, 98.55 and 98.55%) from tap water, sea water and industrial wastewater samples, respectively.
32673717	0	16	link	derived	6:12	arg1	hydrogel					21:28	Novel derived pectin hydrogel	0:28	Novel derived pectin hydrogel from mandarin peel	0:47	Novel derived pectin hydrogel from mandarin peel based metal-organic frameworks composite for enhanced Cr(VI) and Pb(II) ions removal.
32673717	3	17	theme	synthesized	501:511	arg1	composite					517:525	The synthesized PHM composite	497:525	The synthesized PHM composite	497:525	The synthesized PHM composite was characterized by SEM, FT-IR, XRD and N2 adsorption-desorption and investigated for the adsorption of anionic species, Cr(VI) as well as cationic species, Pb(II) ions from aqueous solution.
32673717	6	18	theme	0.1 mol L-1	1163:1173	arg1	HCl					1175:1177	0.1 mol L-1 HCl	1163:1177	0.1 mol L-1 HCl	1163:1177	High swelling capacity (1500.0%) was also characterized for PHM composite after only 400 min and excellent stability for eight cycles with respect to regeneration with 0.1 mol L-1 HCl.
32673717	7	19	theme	water	1245:1249	arg1	matrices					1251:1258	real water matrices	1240:1258	real water matrices	1240:1258	The validity of PHM composite for simultaneous removal from real water matrices were confirmed as Cr(VI) (99.73, 99.87 and 99.87%) and Pb(II) (99.02, 98.55 and 98.55%) from tap water, sea water and industrial wastewater samples, respectively.
32673717	2	20	theme	in	471:472	arg1	method					489:494	in situ synthesis method	471:494	in situ synthesis method	471:494	The pectin hydrogel (PH) was derived from mandarin orange peels by-products and calcium chloride was applied as a cross-linker for incorporation with Fe-TAC metal organic frameworks (MOFs) for the formation of PHM composite on in situ synthesis method.
32673717	0	21	from	peel	44:47	arg1	hydrogel					21:28	Novel derived pectin hydrogel	0:28	Novel derived pectin hydrogel from mandarin peel	0:47	Novel derived pectin hydrogel from mandarin peel based metal-organic frameworks composite for enhanced Cr(VI) and Pb(II) ions removal.
32673717	6	22	theme	excellent	1092:1100	arg1	stability					1102:1110	excellent stability	1092:1110	excellent stability	1092:1110	High swelling capacity (1500.0%) was also characterized for PHM composite after only 400 min and excellent stability for eight cycles with respect to regeneration with 0.1 mol L-1 HCl.
32673717	0	23	dep	Cr	103:104	arg1	removal					126:132	removal	126:132	removal	126:132	Novel derived pectin hydrogel from mandarin peel based metal-organic frameworks composite for enhanced Cr(VI) and Pb(II) ions removal.
32673717	0	23	dep	Cr	103:104	arg1	VI					106:107	VI	106:107	VI	106:107	Novel derived pectin hydrogel from mandarin peel based metal-organic frameworks composite for enhanced Cr(VI) and Pb(II) ions removal.
32673717	2	24	theme	pectin	248:253	arg1	PH					265:266	PH	265:266	PH	265:266	The pectin hydrogel (PH) was derived from mandarin orange peels by-products and calcium chloride was applied as a cross-linker for incorporation with Fe-TAC metal organic frameworks (MOFs) for the formation of PHM composite on in situ synthesis method.
32673717	2	24	theme	pectin	248:253	arg1	hydrogel					255:262	The pectin hydrogel	244:262	The pectin hydrogel (PH)	244:267	The pectin hydrogel (PH) was derived from mandarin orange peels by-products and calcium chloride was applied as a cross-linker for incorporation with Fe-TAC metal organic frameworks (MOFs) for the formation of PHM composite on in situ synthesis method.
32673717	4	25	theme	equilibrium	830:840	arg1	data					853:856	the equilibrium adsorption data	826:856	the equilibrium adsorption data	826:856	The adsorption kinetics for Cr(VI)/Pb(II) ions removal followed the pseudo-second order kinetic model and the equilibrium adsorption data were well fitted by Langmuir model.
32673717	0	26	theme	derived	6:12	arg1	hydrogel					21:28	Novel derived pectin hydrogel	0:28	Novel derived pectin hydrogel from mandarin peel	0:47	Novel derived pectin hydrogel from mandarin peel based metal-organic frameworks composite for enhanced Cr(VI) and Pb(II) ions removal.
32673717	3	27	theme	species	676:682	arg1	adsorption					618:627	the adsorption	614:627	the adsorption of anionic species, Cr(VI) as well as cationic species, Pb(II) ions from aqueous solution	614:717	The synthesized PHM composite was characterized by SEM, FT-IR, XRD and N2 adsorption-desorption and investigated for the adsorption of anionic species, Cr(VI) as well as cationic species, Pb(II) ions from aqueous solution.
32673717	6	28	theme	swelling	1000:1007	arg1	capacity					1009:1016	High swelling capacity	995:1016	High swelling capacity (1500.0%)	995:1026	High swelling capacity (1500.0%) was also characterized for PHM composite after only 400 min and excellent stability for eight cycles with respect to regeneration with 0.1 mol L-1 HCl.
32673717	6	28	theme	swelling	1000:1007	arg1	%					1025:1025	1500.0%	1019:1025	1500.0%	1019:1025	High swelling capacity (1500.0%) was also characterized for PHM composite after only 400 min and excellent stability for eight cycles with respect to regeneration with 0.1 mol L-1 HCl.
32673717	1	29	theme	hydrogels-metal	139:153	arg1	materials					181:189	The hydrogels-metal organic frameworks hybrid materials	135:189	The hydrogels-metal organic frameworks hybrid materials	135:189	The hydrogels-metal organic frameworks hybrid materials have received increasing attentions in recent years.
32673717	0	30	theme	Novel	0:4	arg1	hydrogel					21:28	Novel derived pectin hydrogel	0:28	Novel derived pectin hydrogel from mandarin peel	0:47	Novel derived pectin hydrogel from mandarin peel based metal-organic frameworks composite for enhanced Cr(VI) and Pb(II) ions removal.
32673717	2	31	attach	derived	273:279	arg1	by-products					308:318	mandarin orange peels by-products	286:318	mandarin orange peels by-products	286:318	The pectin hydrogel (PH) was derived from mandarin orange peels by-products and calcium chloride was applied as a cross-linker for incorporation with Fe-TAC metal organic frameworks (MOFs) for the formation of PHM composite on in situ synthesis method.
32673717	2	31	attach	derived	273:279	arg2	PH					265:266	PH	265:266	PH	265:266	The pectin hydrogel (PH) was derived from mandarin orange peels by-products and calcium chloride was applied as a cross-linker for incorporation with Fe-TAC metal organic frameworks (MOFs) for the formation of PHM composite on in situ synthesis method.
32673717	2	31	attach	derived	273:279	arg2	hydrogel					255:262	The pectin hydrogel	244:262	The pectin hydrogel (PH)	244:267	The pectin hydrogel (PH) was derived from mandarin orange peels by-products and calcium chloride was applied as a cross-linker for incorporation with Fe-TAC metal organic frameworks (MOFs) for the formation of PHM composite on in situ synthesis method.
32673717	6	32	theme	only	1075:1078	arg1	400 min					1080:1086	only 400 min	1075:1086	only 400 min	1075:1086	High swelling capacity (1500.0%) was also characterized for PHM composite after only 400 min and excellent stability for eight cycles with respect to regeneration with 0.1 mol L-1 HCl.
32673717	6	33	theme	High	995:998	arg1	capacity					1009:1016	High swelling capacity	995:1016	High swelling capacity (1500.0%)	995:1026	High swelling capacity (1500.0%) was also characterized for PHM composite after only 400 min and excellent stability for eight cycles with respect to regeneration with 0.1 mol L-1 HCl.
32673717	6	33	theme	High	995:998	arg1	%					1025:1025	1500.0%	1019:1025	1500.0%	1019:1025	High swelling capacity (1500.0%) was also characterized for PHM composite after only 400 min and excellent stability for eight cycles with respect to regeneration with 0.1 mol L-1 HCl.
32673717	1	34	theme	organic	155:161	arg1	materials					181:189	The hydrogels-metal organic frameworks hybrid materials	135:189	The hydrogels-metal organic frameworks hybrid materials	135:189	The hydrogels-metal organic frameworks hybrid materials have received increasing attentions in recent years.
32673717	2	35	theme	organic	407:413	arg1	frameworks					415:424	Fe-TAC metal organic frameworks	394:424	Fe-TAC metal organic frameworks (MOFs) for the formation of PHM composite on in situ synthesis method	394:494	The pectin hydrogel (PH) was derived from mandarin orange peels by-products and calcium chloride was applied as a cross-linker for incorporation with Fe-TAC metal organic frameworks (MOFs) for the formation of PHM composite on in situ synthesis method.
32673717	2	35	theme	organic	407:413	arg1	MOFs					427:430	MOFs	427:430	MOFs	427:430	The pectin hydrogel (PH) was derived from mandarin orange peels by-products and calcium chloride was applied as a cross-linker for incorporation with Fe-TAC metal organic frameworks (MOFs) for the formation of PHM composite on in situ synthesis method.
32673717	7	36	theme	industrial	1378:1387	arg1	samples					1400:1406	industrial wastewater samples	1378:1406	industrial wastewater samples	1378:1406	The validity of PHM composite for simultaneous removal from real water matrices were confirmed as Cr(VI) (99.73, 99.87 and 99.87%) and Pb(II) (99.02, 98.55 and 98.55%) from tap water, sea water and industrial wastewater samples, respectively.
32673717	2	37	dep	in	471:472	arg1	situ					474:477	situ	474:477	situ	474:477	The pectin hydrogel (PH) was derived from mandarin orange peels by-products and calcium chloride was applied as a cross-linker for incorporation with Fe-TAC metal organic frameworks (MOFs) for the formation of PHM composite on in situ synthesis method.
32673717	4	38	theme	adsorption	724:733	arg1	kinetics					735:742	The adsorption kinetics	720:742	The adsorption kinetics for Cr	720:749	The adsorption kinetics for Cr(VI)/Pb(II) ions removal followed the pseudo-second order kinetic model and the equilibrium adsorption data were well fitted by Langmuir model.
32673717	3	39	theme	PHM	513:515	arg1	composite					517:525	The synthesized PHM composite	497:525	The synthesized PHM composite	497:525	The synthesized PHM composite was characterized by SEM, FT-IR, XRD and N2 adsorption-desorption and investigated for the adsorption of anionic species, Cr(VI) as well as cationic species, Pb(II) ions from aqueous solution.
32673717	1	40	theme	frameworks	163:172	arg1	materials					181:189	The hydrogels-metal organic frameworks hybrid materials	135:189	The hydrogels-metal organic frameworks hybrid materials	135:189	The hydrogels-metal organic frameworks hybrid materials have received increasing attentions in recent years.
32673717	0	41	theme	pectin	14:19	arg1	hydrogel					21:28	Novel derived pectin hydrogel	0:28	Novel derived pectin hydrogel from mandarin peel	0:47	Novel derived pectin hydrogel from mandarin peel based metal-organic frameworks composite for enhanced Cr(VI) and Pb(II) ions removal.
32673717	3	42	dep	Cr	649:650	arg1	VI					652:653	VI	652:653	VI	652:653	The synthesized PHM composite was characterized by SEM, FT-IR, XRD and N2 adsorption-desorption and investigated for the adsorption of anionic species, Cr(VI) as well as cationic species, Pb(II) ions from aqueous solution.
32673717	4	43	theme	order	802:806	arg1	model					816:820	the pseudo-second order kinetic model	784:820	the pseudo-second order kinetic model	784:820	The adsorption kinetics for Cr(VI)/Pb(II) ions removal followed the pseudo-second order kinetic model and the equilibrium adsorption data were well fitted by Langmuir model.
32673717	7	44	theme	real	1240:1243	arg1	matrices					1251:1258	real water matrices	1240:1258	real water matrices	1240:1258	The validity of PHM composite for simultaneous removal from real water matrices were confirmed as Cr(VI) (99.73, 99.87 and 99.87%) and Pb(II) (99.02, 98.55 and 98.55%) from tap water, sea water and industrial wastewater samples, respectively.
32673717	6	45	with	regeneration	1145:1156	arg1	HCl					1175:1177	0.1 mol L-1 HCl	1163:1177	0.1 mol L-1 HCl	1163:1177	High swelling capacity (1500.0%) was also characterized for PHM composite after only 400 min and excellent stability for eight cycles with respect to regeneration with 0.1 mol L-1 HCl.
32673717	1	46	theme	hybrid	174:179	arg1	materials					181:189	The hydrogels-metal organic frameworks hybrid materials	135:189	The hydrogels-metal organic frameworks hybrid materials	135:189	The hydrogels-metal organic frameworks hybrid materials have received increasing attentions in recent years.
32673717	0	47	theme	mandarin	35:42	arg1	peel					44:47	mandarin peel	35:47	mandarin peel	35:47	Novel derived pectin hydrogel from mandarin peel based metal-organic frameworks composite for enhanced Cr(VI) and Pb(II) ions removal.
32673717	3	48	theme	cationic	667:674	arg1	species					676:682	cationic species	667:682	cationic species	667:682	The synthesized PHM composite was characterized by SEM, FT-IR, XRD and N2 adsorption-desorption and investigated for the adsorption of anionic species, Cr(VI) as well as cationic species, Pb(II) ions from aqueous solution.
32673717	4	49	theme	kinetic	808:814	arg1	model					816:820	the pseudo-second order kinetic model	784:820	the pseudo-second order kinetic model	784:820	The adsorption kinetics for Cr(VI)/Pb(II) ions removal followed the pseudo-second order kinetic model and the equilibrium adsorption data were well fitted by Langmuir model.
32673717	2	50	from	formation	441:449	arg1	method					489:494	in situ synthesis method	471:494	in situ synthesis method	471:494	The pectin hydrogel (PH) was derived from mandarin orange peels by-products and calcium chloride was applied as a cross-linker for incorporation with Fe-TAC metal organic frameworks (MOFs) for the formation of PHM composite on in situ synthesis method.
32673717	7	51	theme	simultaneous	1214:1225	arg1	removal					1227:1233	simultaneous removal	1214:1233	simultaneous removal from real water matrices	1214:1258	The validity of PHM composite for simultaneous removal from real water matrices were confirmed as Cr(VI) (99.73, 99.87 and 99.87%) and Pb(II) (99.02, 98.55 and 98.55%) from tap water, sea water and industrial wastewater samples, respectively.
32673717	0	52	theme	Pb	114:115	arg1	ions					121:124	Pb(II) ions	114:124	Pb(II) ions	114:124	Novel derived pectin hydrogel from mandarin peel based metal-organic frameworks composite for enhanced Cr(VI) and Pb(II) ions removal.
32673717	5	53	theme	/Pb	937:939	arg1	ions					945:948	/Pb(II) ions	937:948	/Pb(II) ions	937:948	The maximum adsorption capacities of Cr(VI)/Pb(II) ions were 825.97 and 913.88 mg g-1, respectively.
32673717	7	54	theme	wastewater	1389:1398	arg1	samples					1400:1406	industrial wastewater samples	1378:1406	industrial wastewater samples	1378:1406	The validity of PHM composite for simultaneous removal from real water matrices were confirmed as Cr(VI) (99.73, 99.87 and 99.87%) and Pb(II) (99.02, 98.55 and 98.55%) from tap water, sea water and industrial wastewater samples, respectively.
32673717	4	55	theme	ions	762:765	arg1	removal					767:773	/Pb(II) ions removal	754:773	/Pb(II) ions removal	754:773	The adsorption kinetics for Cr(VI)/Pb(II) ions removal followed the pseudo-second order kinetic model and the equilibrium adsorption data were well fitted by Langmuir model.
32673717	2	56	theme	PHM	454:456	arg1	composite					458:466	PHM composite	454:466	PHM composite	454:466	The pectin hydrogel (PH) was derived from mandarin orange peels by-products and calcium chloride was applied as a cross-linker for incorporation with Fe-TAC metal organic frameworks (MOFs) for the formation of PHM composite on in situ synthesis method.
32673717	5	57	theme	maximum	898:904	arg1	capacities					917:926	The maximum adsorption capacities	894:926	The maximum adsorption capacities of Cr	894:932	The maximum adsorption capacities of Cr(VI)/Pb(II) ions were 825.97 and 913.88 mg g-1, respectively.
32673717	5	57	theme	maximum	898:904	arg1	825.97					955:960	825.97	955:960	825.97	955:960	The maximum adsorption capacities of Cr(VI)/Pb(II) ions were 825.97 and 913.88 mg g-1, respectively.
32673717	4	58	theme	Langmuir	878:885	arg1	model					887:891	Langmuir model	878:891	Langmuir model	878:891	The adsorption kinetics for Cr(VI)/Pb(II) ions removal followed the pseudo-second order kinetic model and the equilibrium adsorption data were well fitted by Langmuir model.
32673717	3	59	theme	Pb	685:686	arg1	ions					692:695	Pb(II) ions	685:695	Pb(II) ions from aqueous solution	685:717	The synthesized PHM composite was characterized by SEM, FT-IR, XRD and N2 adsorption-desorption and investigated for the adsorption of anionic species, Cr(VI) as well as cationic species, Pb(II) ions from aqueous solution.
32673717	0	60	theme	II	117:118	arg1	ions					121:124	Pb(II) ions	114:124	Pb(II) ions	114:124	Novel derived pectin hydrogel from mandarin peel based metal-organic frameworks composite for enhanced Cr(VI) and Pb(II) ions removal.
32673717	5	61	theme	adsorption	906:915	arg1	capacities					917:926	The maximum adsorption capacities	894:926	The maximum adsorption capacities of Cr	894:932	The maximum adsorption capacities of Cr(VI)/Pb(II) ions were 825.97 and 913.88 mg g-1, respectively.
32673717	5	61	theme	adsorption	906:915	arg1	825.97					955:960	825.97	955:960	825.97	955:960	The maximum adsorption capacities of Cr(VI)/Pb(II) ions were 825.97 and 913.88 mg g-1, respectively.
32673717	4	62	theme	adsorption	842:851	arg1	data					853:856	the equilibrium adsorption data	826:856	the equilibrium adsorption data	826:856	The adsorption kinetics for Cr(VI)/Pb(II) ions removal followed the pseudo-second order kinetic model and the equilibrium adsorption data were well fitted by Langmuir model.
32673717	2	63	theme	peels	302:306	arg1	by-products					308:318	mandarin orange peels by-products	286:318	mandarin orange peels by-products	286:318	The pectin hydrogel (PH) was derived from mandarin orange peels by-products and calcium chloride was applied as a cross-linker for incorporation with Fe-TAC metal organic frameworks (MOFs) for the formation of PHM composite on in situ synthesis method.
32673717	7	64	theme	tap	1353:1355	arg1	water					1357:1361	tap water	1353:1361	tap water	1353:1361	The validity of PHM composite for simultaneous removal from real water matrices were confirmed as Cr(VI) (99.73, 99.87 and 99.87%) and Pb(II) (99.02, 98.55 and 98.55%) from tap water, sea water and industrial wastewater samples, respectively.
32673717	3	65	theme	anionic	632:638	arg1	species					640:646	anionic species	632:646	anionic species	632:646	The synthesized PHM composite was characterized by SEM, FT-IR, XRD and N2 adsorption-desorption and investigated for the adsorption of anionic species, Cr(VI) as well as cationic species, Pb(II) ions from aqueous solution.
32673717	2	66	theme	orange	295:300	arg1	by-products					308:318	mandarin orange peels by-products	286:318	mandarin orange peels by-products	286:318	The pectin hydrogel (PH) was derived from mandarin orange peels by-products and calcium chloride was applied as a cross-linker for incorporation with Fe-TAC metal organic frameworks (MOFs) for the formation of PHM composite on in situ synthesis method.
32673717	3	67	theme	aqueous	702:708	arg1	solution					710:717	aqueous solution	702:717	aqueous solution	702:717	The synthesized PHM composite was characterized by SEM, FT-IR, XRD and N2 adsorption-desorption and investigated for the adsorption of anionic species, Cr(VI) as well as cationic species, Pb(II) ions from aqueous solution.
32673717	3	68	from	solution	710:717	arg1	Cr					649:650	Cr	649:650	Cr(VI)	649:654	The synthesized PHM composite was characterized by SEM, FT-IR, XRD and N2 adsorption-desorption and investigated for the adsorption of anionic species, Cr(VI) as well as cationic species, Pb(II) ions from aqueous solution.
32673717	3	68	from	solution	710:717	arg1	adsorption					618:627	the adsorption	614:627	the adsorption of anionic species, Cr(VI) as well as cationic species, Pb(II) ions from aqueous solution	614:717	The synthesized PHM composite was characterized by SEM, FT-IR, XRD and N2 adsorption-desorption and investigated for the adsorption of anionic species, Cr(VI) as well as cationic species, Pb(II) ions from aqueous solution.
32673717	3	68	from	solution	710:717	arg1	species					640:646	anionic species	632:646	anionic species	632:646	The synthesized PHM composite was characterized by SEM, FT-IR, XRD and N2 adsorption-desorption and investigated for the adsorption of anionic species, Cr(VI) as well as cationic species, Pb(II) ions from aqueous solution.
32673717	3	68	from	solution	710:717	arg1	ions					692:695	Pb(II) ions	685:695	Pb(II) ions from aqueous solution	685:717	The synthesized PHM composite was characterized by SEM, FT-IR, XRD and N2 adsorption-desorption and investigated for the adsorption of anionic species, Cr(VI) as well as cationic species, Pb(II) ions from aqueous solution.
32673717	3	68	from	solution	710:717	arg1	species					676:682	cationic species	667:682	cationic species	667:682	The synthesized PHM composite was characterized by SEM, FT-IR, XRD and N2 adsorption-desorption and investigated for the adsorption of anionic species, Cr(VI) as well as cationic species, Pb(II) ions from aqueous solution.
32673717	5	69	theme	Cr	931:932	arg1	capacities					917:926	The maximum adsorption capacities	894:926	The maximum adsorption capacities of Cr	894:932	The maximum adsorption capacities of Cr(VI)/Pb(II) ions were 825.97 and 913.88 mg g-1, respectively.
32673717	5	69	theme	Cr	931:932	arg1	825.97					955:960	825.97	955:960	825.97	955:960	The maximum adsorption capacities of Cr(VI)/Pb(II) ions were 825.97 and 913.88 mg g-1, respectively.
32673717	3	70	theme	N2	568:569	arg1	adsorption-desorption					571:591	N2 adsorption-desorption	568:591	N2 adsorption-desorption	568:591	The synthesized PHM composite was characterized by SEM, FT-IR, XRD and N2 adsorption-desorption and investigated for the adsorption of anionic species, Cr(VI) as well as cationic species, Pb(II) ions from aqueous solution.
32673717	2	71	theme	mandarin	286:293	arg1	by-products					308:318	mandarin orange peels by-products	286:318	mandarin orange peels by-products	286:318	The pectin hydrogel (PH) was derived from mandarin orange peels by-products and calcium chloride was applied as a cross-linker for incorporation with Fe-TAC metal organic frameworks (MOFs) for the formation of PHM composite on in situ synthesis method.
32673717	1	72	theme	increasing	205:214	arg1	attentions					216:225	increasing attentions	205:225	increasing attentions	205:225	The hydrogels-metal organic frameworks hybrid materials have received increasing attentions in recent years.
32673717	2	73	theme	composite	458:466	arg1	formation					441:449	the formation	437:449	the formation of PHM composite on in situ synthesis method	437:494	The pectin hydrogel (PH) was derived from mandarin orange peels by-products and calcium chloride was applied as a cross-linker for incorporation with Fe-TAC metal organic frameworks (MOFs) for the formation of PHM composite on in situ synthesis method.
32673717	4	74	theme	pseudo-second	788:800	arg1	model					816:820	the pseudo-second order kinetic model	784:820	the pseudo-second order kinetic model	784:820	The adsorption kinetics for Cr(VI)/Pb(II) ions removal followed the pseudo-second order kinetic model and the equilibrium adsorption data were well fitted by Langmuir model.
32673717	2	75	theme	Fe-TAC	394:399	arg1	frameworks					415:424	Fe-TAC metal organic frameworks	394:424	Fe-TAC metal organic frameworks (MOFs) for the formation of PHM composite on in situ synthesis method	394:494	The pectin hydrogel (PH) was derived from mandarin orange peels by-products and calcium chloride was applied as a cross-linker for incorporation with Fe-TAC metal organic frameworks (MOFs) for the formation of PHM composite on in situ synthesis method.
32673717	2	75	theme	Fe-TAC	394:399	arg1	MOFs					427:430	MOFs	427:430	MOFs	427:430	The pectin hydrogel (PH) was derived from mandarin orange peels by-products and calcium chloride was applied as a cross-linker for incorporation with Fe-TAC metal organic frameworks (MOFs) for the formation of PHM composite on in situ synthesis method.
32673717	7	76	from	matrices	1251:1258	arg1	removal					1227:1233	simultaneous removal	1214:1233	simultaneous removal from real water matrices	1214:1258	The validity of PHM composite for simultaneous removal from real water matrices were confirmed as Cr(VI) (99.73, 99.87 and 99.87%) and Pb(II) (99.02, 98.55 and 98.55%) from tap water, sea water and industrial wastewater samples, respectively.
32673717	3	77	theme	ions	692:695	arg1	adsorption					618:627	the adsorption	614:627	the adsorption of anionic species, Cr(VI) as well as cationic species, Pb(II) ions from aqueous solution	614:717	The synthesized PHM composite was characterized by SEM, FT-IR, XRD and N2 adsorption-desorption and investigated for the adsorption of anionic species, Cr(VI) as well as cationic species, Pb(II) ions from aqueous solution.
32673717	0	78	theme	metal-organic	55:67	arg1	frameworks					69:78	metal-organic frameworks	55:78	metal-organic frameworks composite for enhanced Cr(VI) and Pb(II) ions removal	55:132	Novel derived pectin hydrogel from mandarin peel based metal-organic frameworks composite for enhanced Cr(VI) and Pb(II) ions removal.
32673717	3	79	theme	Cr	649:650	arg1	adsorption					618:627	the adsorption	614:627	the adsorption of anionic species, Cr(VI) as well as cationic species, Pb(II) ions from aqueous solution	614:717	The synthesized PHM composite was characterized by SEM, FT-IR, XRD and N2 adsorption-desorption and investigated for the adsorption of anionic species, Cr(VI) as well as cationic species, Pb(II) ions from aqueous solution.
32673717	2	80	theme	metal	401:405	arg1	frameworks					415:424	Fe-TAC metal organic frameworks	394:424	Fe-TAC metal organic frameworks (MOFs) for the formation of PHM composite on in situ synthesis method	394:494	The pectin hydrogel (PH) was derived from mandarin orange peels by-products and calcium chloride was applied as a cross-linker for incorporation with Fe-TAC metal organic frameworks (MOFs) for the formation of PHM composite on in situ synthesis method.
32673717	2	80	theme	metal	401:405	arg1	MOFs					427:430	MOFs	427:430	MOFs	427:430	The pectin hydrogel (PH) was derived from mandarin orange peels by-products and calcium chloride was applied as a cross-linker for incorporation with Fe-TAC metal organic frameworks (MOFs) for the formation of PHM composite on in situ synthesis method.
34710513	0	0	theme	gut	78:80	arg1	ecosystem					92:100	the synthetic gut microbial ecosystem	64:100	the synthetic gut microbial ecosystem	64:100	Simulation of the mucosal environment in the re-construction of the synthetic gut microbial ecosystem.
34710513	7	1	theme	research	1496:1503	arg1	translation					1470:1480	easy translation	1465:1480	easy translation of microbiome research to therapies	1465:1516	The current results demonstrate that the simulation of the gut microbial ecosystem in vitro can be beneficial to the mucosal environment mimicking and the study on the mechanistic potential of the human gut microbiota for easy translation of microbiome research to therapies.
34710513	6	2	theme	medium	995:1000	arg1	part					1002:1005	each medium part	990:1005	each medium part	990:1005	Metabolic functional prediction for the microbial community in each medium part showed that the gene expression involved in metabolism and cell motility pathways were distinctively differentiated between the liquid and solid medium part, and the functional potential was highly related to the microbial composition.
34710513	0	3	theme	synthetic	68:76	arg1	ecosystem					92:100	the synthetic gut microbial ecosystem	64:100	the synthetic gut microbial ecosystem	64:100	Simulation of the mucosal environment in the re-construction of the synthetic gut microbial ecosystem.
34710513	7	4	theme	current	1247:1253	arg1	results					1255:1261	The current results	1243:1261	The current results	1243:1261	The current results demonstrate that the simulation of the gut microbial ecosystem in vitro can be beneficial to the mucosal environment mimicking and the study on the mechanistic potential of the human gut microbiota for easy translation of microbiome research to therapies.
34710513	0	5	theme	ecosystem	92:100	arg1	re-construction					45:59	the re-construction	41:59	the re-construction of the synthetic gut microbial ecosystem	41:100	Simulation of the mucosal environment in the re-construction of the synthetic gut microbial ecosystem.
34710513	0	6	from	Simulation	0:9	arg1	re-construction					45:59	the re-construction	41:59	the re-construction of the synthetic gut microbial ecosystem	41:100	Simulation of the mucosal environment in the re-construction of the synthetic gut microbial ecosystem.
34710513	2	7	theme	microbial	300:308	arg1	community					310:318	mucin-agar gel and synthetic mucosal microbial community	263:318	community	310:318	This study sought to simulate the mucosal environment using mucin-agar gel and synthetic mucosal microbial community in vitro.
34710513	6	8	theme	solid	1146:1150	arg1	part					1159:1162	the liquid and solid medium part	1131:1162	the liquid and solid medium part	1131:1162	Metabolic functional prediction for the microbial community in each medium part showed that the gene expression involved in metabolism and cell motility pathways were distinctively differentiated between the liquid and solid medium part, and the functional potential was highly related to the microbial composition.
34710513	0	9	theme	microbial	82:90	arg1	ecosystem					92:100	the synthetic gut microbial ecosystem	64:100	the synthetic gut microbial ecosystem	64:100	Simulation of the mucosal environment in the re-construction of the synthetic gut microbial ecosystem.
34710513	6	10	theme	functional	1173:1182	arg1	related					1205:1211	related	1205:1211	related	1205:1211	Metabolic functional prediction for the microbial community in each medium part showed that the gene expression involved in metabolism and cell motility pathways were distinctively differentiated between the liquid and solid medium part, and the functional potential was highly related to the microbial composition.
34710513	6	10	theme	functional	1173:1182	arg1	potential					1184:1192	the functional potential	1169:1192	the functional potential	1169:1192	Metabolic functional prediction for the microbial community in each medium part showed that the gene expression involved in metabolism and cell motility pathways were distinctively differentiated between the liquid and solid medium part, and the functional potential was highly related to the microbial composition.
34710513	2	11	theme	mucosal	292:298	arg1	community					310:318	mucin-agar gel and synthetic mucosal microbial community	263:318	community	310:318	This study sought to simulate the mucosal environment using mucin-agar gel and synthetic mucosal microbial community in vitro.
34710513	3	12	dep	Gifu	506:509	arg1	selected					538:545	selected	538:545	were selected considering their microbial biomass and bacterial composition	533:607	To select suitable culture media, microbial communities were assembled and cultured in seven different media at 37 °C for 36 h. Among the seven media, Bryant & Burkey (BB) and Gifu Anaerobic Media (GAM) were selected considering their microbial biomass and bacterial composition.
34710513	2	13	theme	synthetic	282:290	arg1	community					310:318	mucin-agar gel and synthetic mucosal microbial community	263:318	community	310:318	This study sought to simulate the mucosal environment using mucin-agar gel and synthetic mucosal microbial community in vitro.
34710513	1	14	theme	human	176:180	arg1	health					182:187	human health	176:187	human health	176:187	Human gut surface-attached mucosal microbiota plays significant roles in human health and diseases.
34710513	5	15	theme	bacterial	725:733	arg1	Roseburia					783:791	Roseburia	783:791	Roseburia	783:791	The results showed that some bacterial genus such as Bifidobacterium, Collinsella, and Roseburia could efficiently colonize in the solid mucin-agar part while Enterococcus, Clostridium, and Veilonella dominated in the liquid part.
34710513	5	15	theme	bacterial	725:733	arg1	Collinsella					766:776	Collinsella	766:776	Collinsella	766:776	The results showed that some bacterial genus such as Bifidobacterium, Collinsella, and Roseburia could efficiently colonize in the solid mucin-agar part while Enterococcus, Clostridium, and Veilonella dominated in the liquid part.
34710513	5	15	theme	bacterial	725:733	arg1	genus					735:739	some bacterial genus	720:739	some bacterial genus such as Bifidobacterium, Collinsella, and Roseburia	720:791	The results showed that some bacterial genus such as Bifidobacterium, Collinsella, and Roseburia could efficiently colonize in the solid mucin-agar part while Enterococcus, Clostridium, and Veilonella dominated in the liquid part.
34710513	5	15	theme	bacterial	725:733	arg1	Bifidobacterium					749:763	Bifidobacterium	749:763	Bifidobacterium	749:763	The results showed that some bacterial genus such as Bifidobacterium, Collinsella, and Roseburia could efficiently colonize in the solid mucin-agar part while Enterococcus, Clostridium, and Veilonella dominated in the liquid part.
34710513	7	16	theme	gut	1446:1448	arg1	microbiota					1450:1459	the human gut microbiota	1436:1459	the human gut microbiota	1436:1459	The current results demonstrate that the simulation of the gut microbial ecosystem in vitro can be beneficial to the mucosal environment mimicking and the study on the mechanistic potential of the human gut microbiota for easy translation of microbiome research to therapies.
34710513	6	17	from	community	977:985	arg1	part					1002:1005	each medium part	990:1005	each medium part	990:1005	Metabolic functional prediction for the microbial community in each medium part showed that the gene expression involved in metabolism and cell motility pathways were distinctively differentiated between the liquid and solid medium part, and the functional potential was highly related to the microbial composition.
34710513	3	18	theme	Anaerobic	511:519	arg1	Media					521:525	Anaerobic Media	511:525	Anaerobic Media (GAM)	511:531	To select suitable culture media, microbial communities were assembled and cultured in seven different media at 37 °C for 36 h. Among the seven media, Bryant & Burkey (BB) and Gifu Anaerobic Media (GAM) were selected considering their microbial biomass and bacterial composition.
34710513	3	18	theme	Anaerobic	511:519	arg1	GAM					528:530	GAM	528:530	GAM	528:530	To select suitable culture media, microbial communities were assembled and cultured in seven different media at 37 °C for 36 h. Among the seven media, Bryant & Burkey (BB) and Gifu Anaerobic Media (GAM) were selected considering their microbial biomass and bacterial composition.
34710513	6	19	theme	cell	1066:1069	arg1	motility					1071:1078	cell motility	1066:1078	cell motility	1066:1078	Metabolic functional prediction for the microbial community in each medium part showed that the gene expression involved in metabolism and cell motility pathways were distinctively differentiated between the liquid and solid medium part, and the functional potential was highly related to the microbial composition.
34710513	7	20	theme	microbiota	1450:1459	arg1	potential					1423:1431	the mechanistic potential	1407:1431	the mechanistic potential of the human gut microbiota for easy translation of microbiome research to therapies	1407:1516	The current results demonstrate that the simulation of the gut microbial ecosystem in vitro can be beneficial to the mucosal environment mimicking and the study on the mechanistic potential of the human gut microbiota for easy translation of microbiome research to therapies.
34710513	7	21	theme	easy	1465:1468	arg1	translation					1470:1480	easy translation	1465:1480	easy translation of microbiome research to therapies	1465:1516	The current results demonstrate that the simulation of the gut microbial ecosystem in vitro can be beneficial to the mucosal environment mimicking and the study on the mechanistic potential of the human gut microbiota for easy translation of microbiome research to therapies.
34710513	6	22	theme	microbial	967:975	arg1	community					977:985	the microbial community	963:985	the microbial community in each medium part	963:1005	Metabolic functional prediction for the microbial community in each medium part showed that the gene expression involved in metabolism and cell motility pathways were distinctively differentiated between the liquid and solid medium part, and the functional potential was highly related to the microbial composition.
34710513	6	23	dep	metabolism	1051:1060	arg1	pathways					1080:1087	pathways	1080:1087	pathways	1080:1087	Metabolic functional prediction for the microbial community in each medium part showed that the gene expression involved in metabolism and cell motility pathways were distinctively differentiated between the liquid and solid medium part, and the functional potential was highly related to the microbial composition.
34710513	7	24	theme	mucosal	1360:1366	arg1	environment					1368:1378	the mucosal environment	1356:1378	the mucosal environment mimicking and the study on the mechanistic potential of the human gut microbiota for easy translation of microbiome research to therapies	1356:1516	The current results demonstrate that the simulation of the gut microbial ecosystem in vitro can be beneficial to the mucosal environment mimicking and the study on the mechanistic potential of the human gut microbiota for easy translation of microbiome research to therapies.
34710513	7	25	theme	human	1440:1444	arg1	microbiota					1450:1459	the human gut microbiota	1436:1459	the human gut microbiota	1436:1459	The current results demonstrate that the simulation of the gut microbial ecosystem in vitro can be beneficial to the mucosal environment mimicking and the study on the mechanistic potential of the human gut microbiota for easy translation of microbiome research to therapies.
34710513	0	26	theme	mucosal	18:24	arg1	environment					26:36	the mucosal environment	14:36	the mucosal environment	14:36	Simulation of the mucosal environment in the re-construction of the synthetic gut microbial ecosystem.
34710513	7	27	theme	mechanistic	1411:1421	arg1	potential					1423:1431	the mechanistic potential	1407:1431	the mechanistic potential of the human gut microbiota for easy translation of microbiome research to therapies	1407:1516	The current results demonstrate that the simulation of the gut microbial ecosystem in vitro can be beneficial to the mucosal environment mimicking and the study on the mechanistic potential of the human gut microbiota for easy translation of microbiome research to therapies.
34710513	6	28	theme	medium	1152:1157	arg1	part					1159:1162	the liquid and solid medium part	1131:1162	the liquid and solid medium part	1131:1162	Metabolic functional prediction for the microbial community in each medium part showed that the gene expression involved in metabolism and cell motility pathways were distinctively differentiated between the liquid and solid medium part, and the functional potential was highly related to the microbial composition.
34710513	3	29	theme	microbial	565:573	arg1	composition					597:607	their microbial biomass and bacterial composition	559:607	their microbial biomass and bacterial composition	559:607	To select suitable culture media, microbial communities were assembled and cultured in seven different media at 37 °C for 36 h. Among the seven media, Bryant & Burkey (BB) and Gifu Anaerobic Media (GAM) were selected considering their microbial biomass and bacterial composition.
34710513	7	30	theme	microbial	1306:1314	arg1	ecosystem					1316:1324	the gut microbial ecosystem	1298:1324	the gut microbial ecosystem	1298:1324	The current results demonstrate that the simulation of the gut microbial ecosystem in vitro can be beneficial to the mucosal environment mimicking and the study on the mechanistic potential of the human gut microbiota for easy translation of microbiome research to therapies.
34710513	7	31	theme	gut	1302:1304	arg1	ecosystem					1316:1324	the gut microbial ecosystem	1298:1324	the gut microbial ecosystem	1298:1324	The current results demonstrate that the simulation of the gut microbial ecosystem in vitro can be beneficial to the mucosal environment mimicking and the study on the mechanistic potential of the human gut microbiota for easy translation of microbiome research to therapies.
34710513	1	32	theme	Human	103:107	arg1	microbiota					138:147	Human gut surface-attached mucosal microbiota	103:147	Human gut surface-attached mucosal microbiota	103:147	Human gut surface-attached mucosal microbiota plays significant roles in human health and diseases.
34710513	7	33	theme	ecosystem	1316:1324	arg1	beneficial					1342:1351	beneficial	1342:1351	beneficial	1342:1351	The current results demonstrate that the simulation of the gut microbial ecosystem in vitro can be beneficial to the mucosal environment mimicking and the study on the mechanistic potential of the human gut microbiota for easy translation of microbiome research to therapies.
34710513	7	33	theme	ecosystem	1316:1324	arg1	simulation					1284:1293	the simulation	1280:1293	the simulation of the gut microbial ecosystem in vitro	1280:1333	The current results demonstrate that the simulation of the gut microbial ecosystem in vitro can be beneficial to the mucosal environment mimicking and the study on the mechanistic potential of the human gut microbiota for easy translation of microbiome research to therapies.
34710513	3	34	theme	different	423:431	arg1	media					433:437	seven different media	417:437	seven different media	417:437	To select suitable culture media, microbial communities were assembled and cultured in seven different media at 37 °C for 36 h. Among the seven media, Bryant & Burkey (BB) and Gifu Anaerobic Media (GAM) were selected considering their microbial biomass and bacterial composition.
34710513	6	35	theme	liquid	1135:1140	arg1	part					1159:1162	the liquid and solid medium part	1131:1162	the liquid and solid medium part	1131:1162	Metabolic functional prediction for the microbial community in each medium part showed that the gene expression involved in metabolism and cell motility pathways were distinctively differentiated between the liquid and solid medium part, and the functional potential was highly related to the microbial composition.
34710513	2	36	theme	mucosal	237:243	arg1	environment					245:255	the mucosal environment	233:255	the mucosal environment	233:255	This study sought to simulate the mucosal environment using mucin-agar gel and synthetic mucosal microbial community in vitro.
34710513	5	37	theme	solid	827:831	arg1	part					844:847	the solid mucin-agar part	823:847	the solid mucin-agar part	823:847	The results showed that some bacterial genus such as Bifidobacterium, Collinsella, and Roseburia could efficiently colonize in the solid mucin-agar part while Enterococcus, Clostridium, and Veilonella dominated in the liquid part.
34710513	0	38	theme	environment	26:36	arg1	Simulation					0:9	Simulation	0:9	Simulation of the mucosal environment in the re-construction of the synthetic gut microbial ecosystem.	0:101	Simulation of the mucosal environment in the re-construction of the synthetic gut microbial ecosystem.
34710513	6	39	theme	functional	937:946	arg1	prediction					948:957	Metabolic functional prediction	927:957	Metabolic functional prediction for the microbial community in each medium part	927:1005	Metabolic functional prediction for the microbial community in each medium part showed that the gene expression involved in metabolism and cell motility pathways were distinctively differentiated between the liquid and solid medium part, and the functional potential was highly related to the microbial composition.
34710513	3	40	theme	biomass	575:581	arg1	composition					597:607	their microbial biomass and bacterial composition	559:607	their microbial biomass and bacterial composition	559:607	To select suitable culture media, microbial communities were assembled and cultured in seven different media at 37 °C for 36 h. Among the seven media, Bryant & Burkey (BB) and Gifu Anaerobic Media (GAM) were selected considering their microbial biomass and bacterial composition.
34710513	5	41	theme	mucin-agar	833:842	arg1	part					844:847	the solid mucin-agar part	823:847	the solid mucin-agar part	823:847	The results showed that some bacterial genus such as Bifidobacterium, Collinsella, and Roseburia could efficiently colonize in the solid mucin-agar part while Enterococcus, Clostridium, and Veilonella dominated in the liquid part.
34710513	3	42	theme	microbial	364:372	arg1	communities					374:384	microbial communities	364:384	microbial communities	364:384	To select suitable culture media, microbial communities were assembled and cultured in seven different media at 37 °C for 36 h. Among the seven media, Bryant & Burkey (BB) and Gifu Anaerobic Media (GAM) were selected considering their microbial biomass and bacterial composition.
34710513	6	43	theme	microbial	1220:1228	arg1	composition					1230:1240	the microbial composition	1216:1240	the microbial composition	1216:1240	Metabolic functional prediction for the microbial community in each medium part showed that the gene expression involved in metabolism and cell motility pathways were distinctively differentiated between the liquid and solid medium part, and the functional potential was highly related to the microbial composition.
34710513	1	44	theme	gut	109:111	arg1	microbiota					138:147	Human gut surface-attached mucosal microbiota	103:147	Human gut surface-attached mucosal microbiota	103:147	Human gut surface-attached mucosal microbiota plays significant roles in human health and diseases.
34710513	1	45	theme	surface-attached	113:128	arg1	microbiota					138:147	Human gut surface-attached mucosal microbiota	103:147	Human gut surface-attached mucosal microbiota	103:147	Human gut surface-attached mucosal microbiota plays significant roles in human health and diseases.
34710513	7	46	from	study	1398:1402	arg1	potential					1423:1431	the mechanistic potential	1407:1431	the mechanistic potential of the human gut microbiota for easy translation of microbiome research to therapies	1407:1516	The current results demonstrate that the simulation of the gut microbial ecosystem in vitro can be beneficial to the mucosal environment mimicking and the study on the mechanistic potential of the human gut microbiota for easy translation of microbiome research to therapies.
34710513	1	47	theme	mucosal	130:136	arg1	microbiota					138:147	Human gut surface-attached mucosal microbiota	103:147	Human gut surface-attached mucosal microbiota	103:147	Human gut surface-attached mucosal microbiota plays significant roles in human health and diseases.
34710513	2	48	theme	mucin-agar	263:272	arg1	gel					274:276	mucin-agar gel and synthetic mucosal microbial community	263:318	gel	274:276	This study sought to simulate the mucosal environment using mucin-agar gel and synthetic mucosal microbial community in vitro.
34710513	5	49	theme	liquid	914:919	arg1	part					921:924	the liquid part	910:924	the liquid part	910:924	The results showed that some bacterial genus such as Bifidobacterium, Collinsella, and Roseburia could efficiently colonize in the solid mucin-agar part while Enterococcus, Clostridium, and Veilonella dominated in the liquid part.
34710513	3	50	theme	suitable	340:347	arg1	media					357:361	suitable culture media	340:361	suitable culture media	340:361	To select suitable culture media, microbial communities were assembled and cultured in seven different media at 37 °C for 36 h. Among the seven media, Bryant & Burkey (BB) and Gifu Anaerobic Media (GAM) were selected considering their microbial biomass and bacterial composition.
34710513	6	51	theme	gene	1023:1026	arg1	expression					1028:1037	the gene expression	1019:1037	the gene expression involved in metabolism and cell motility pathways	1019:1087	Metabolic functional prediction for the microbial community in each medium part showed that the gene expression involved in metabolism and cell motility pathways were distinctively differentiated between the liquid and solid medium part, and the functional potential was highly related to the microbial composition.
34710513	3	52	theme	culture	349:355	arg1	media					357:361	suitable culture media	340:361	suitable culture media	340:361	To select suitable culture media, microbial communities were assembled and cultured in seven different media at 37 °C for 36 h. Among the seven media, Bryant & Burkey (BB) and Gifu Anaerobic Media (GAM) were selected considering their microbial biomass and bacterial composition.
34710513	7	53	theme	microbiome	1485:1494	arg1	research					1496:1503	microbiome research	1485:1503	microbiome research	1485:1503	The current results demonstrate that the simulation of the gut microbial ecosystem in vitro can be beneficial to the mucosal environment mimicking and the study on the mechanistic potential of the human gut microbiota for easy translation of microbiome research to therapies.
34710513	3	54	theme	bacterial	587:595	arg1	composition					597:607	their microbial biomass and bacterial composition	559:607	their microbial biomass and bacterial composition	559:607	To select suitable culture media, microbial communities were assembled and cultured in seven different media at 37 °C for 36 h. Among the seven media, Bryant & Burkey (BB) and Gifu Anaerobic Media (GAM) were selected considering their microbial biomass and bacterial composition.
34710513	1	55	theme	significant	155:165	arg1	roles					167:171	significant roles	155:171	significant roles	155:171	Human gut surface-attached mucosal microbiota plays significant roles in human health and diseases.
34710513	6	56	theme	Metabolic	927:935	arg1	prediction					948:957	Metabolic functional prediction	927:957	Metabolic functional prediction for the microbial community in each medium part	927:1005	Metabolic functional prediction for the microbial community in each medium part showed that the gene expression involved in metabolism and cell motility pathways were distinctively differentiated between the liquid and solid medium part, and the functional potential was highly related to the microbial composition.
33049811	0	0	theme	antimicrobial	71:83	arg1	activity					85:92	antimicrobial activity	71:92	antimicrobial activity	71:92	Investigation of the structural, physical properties, antioxidant, and antimicrobial activity of chitosan- nano-silicon aerogel composite edible films incorporated with okara powder.
33049811	4	1	theme	film	562:565	arg1	glossiness					533:542	The surface glossiness	521:542	The surface glossiness of C/D/S1.5:1:0.1 film	521:565	The surface glossiness of C/D/S1.5:1:0.1 film was 14.4Gu.
33049811	5	2	theme	antibacterial	590:602	arg1	activity					604:611	the antibacterial activity	586:611	the antibacterial activity	586:611	As for the antibacterial activity, all the composite films had strong antibacterial activity against Gram-negative (E. coli) and Gram-positive (S. aureus), and the inhibition zone of C/D/S1.5:1:0.10 against E. coli reached 551.96 mm2, the inhibition zone for S. aureus was 350.29 mm2.
33049811	5	3	theme	inhibition	743:752	arg1	zone					754:757	the inhibition zone	739:757	the inhibition zone of C/D/S1.5:1:0.10 against E. coli	739:792	As for the antibacterial activity, all the composite films had strong antibacterial activity against Gram-negative (E. coli) and Gram-positive (S. aureus), and the inhibition zone of C/D/S1.5:1:0.10 against E. coli reached 551.96 mm2, the inhibition zone for S. aureus was 350.29 mm2.
33049811	6	4	theme	composite	868:876	arg1	film					878:881	The composite film	864:881	The composite film	864:881	The composite film had uniform, non-porous, continuous and dense surface characteristics.
33049811	5	5	theme	antibacterial	649:661	arg1	activity					663:670	strong antibacterial activity	642:670	strong antibacterial activity against Gram-negative (E. coli) and Gram-positive (S. aureus)	642:732	As for the antibacterial activity, all the composite films had strong antibacterial activity against Gram-negative (E. coli) and Gram-positive (S. aureus), and the inhibition zone of C/D/S1.5:1:0.10 against E. coli reached 551.96 mm2, the inhibition zone for S. aureus was 350.29 mm2.
33049811	6	6	contain	had	883:885	arg1	film					878:881	The composite film	864:881	The composite film	864:881	The composite film had uniform, non-porous, continuous and dense surface characteristics.
33049811	6	6	contain	had	883:885	arg2	characteristics					937:951	uniform, non-porous, continuous and dense surface characteristics	887:951	uniform, non-porous, continuous and dense surface characteristics	887:951	The composite film had uniform, non-porous, continuous and dense surface characteristics.
33049811	5	7	theme	C/D/S1.5:1:0.10	762:776	arg1	zone					754:757	the inhibition zone	739:757	the inhibition zone of C/D/S1.5:1:0.10 against E. coli	739:792	As for the antibacterial activity, all the composite films had strong antibacterial activity against Gram-negative (E. coli) and Gram-positive (S. aureus), and the inhibition zone of C/D/S1.5:1:0.10 against E. coli reached 551.96 mm2, the inhibition zone for S. aureus was 350.29 mm2.
33049811	1	8	theme	physicochemical	287:301	arg1	properties					303:312	their physicochemical properties	281:312	their physicochemical properties	281:312	The chitosan/okra powder/nano-silicon aerogel composite films were prepared by casting method and their physicochemical properties and structural characterization were studied.
33049811	0	9	theme	activity	85:92	arg1	Investigation					0:12	Investigation	0:12	Investigation of the structural, physical properties, antioxidant, and antimicrobial activity of chitosan- nano-silicon aerogel composite edible films	0:149	Investigation of the structural, physical properties, antioxidant, and antimicrobial activity of chitosan- nano-silicon aerogel composite edible films incorporated with okara powder.
33049811	6	10	theme	surface	929:935	arg1	characteristics					937:951	uniform, non-porous, continuous and dense surface characteristics	887:951	uniform, non-porous, continuous and dense surface characteristics	887:951	The composite film had uniform, non-porous, continuous and dense surface characteristics.
33049811	0	11	theme	nano-silicon	107:118	arg1	films					145:149	chitosan- nano-silicon aerogel composite edible films	97:149	chitosan- nano-silicon aerogel composite edible films	97:149	Investigation of the structural, physical properties, antioxidant, and antimicrobial activity of chitosan- nano-silicon aerogel composite edible films incorporated with okara powder.
33049811	2	12	contain	had	403:405	arg2	properties					423:432	good mechanical properties	407:432	good mechanical properties	407:432	The results showed that the composite film had good mechanical properties, barrier properties and optical properties.
33049811	2	12	contain	had	403:405	arg2	properties					466:475	optical properties	458:475	optical properties	458:475	The results showed that the composite film had good mechanical properties, barrier properties and optical properties.
33049811	2	12	contain	had	403:405	arg2	properties					443:452	barrier properties	435:452	barrier properties	435:452	The results showed that the composite film had good mechanical properties, barrier properties and optical properties.
33049811	2	12	contain	had	403:405	arg1	film					398:401	the composite film	384:401	the composite film	384:401	The results showed that the composite film had good mechanical properties, barrier properties and optical properties.
33049811	5	13	contain	had	638:640	arg2	activity					663:670	strong antibacterial activity	642:670	strong antibacterial activity against Gram-negative (E. coli) and Gram-positive (S. aureus)	642:732	As for the antibacterial activity, all the composite films had strong antibacterial activity against Gram-negative (E. coli) and Gram-positive (S. aureus), and the inhibition zone of C/D/S1.5:1:0.10 against E. coli reached 551.96 mm2, the inhibition zone for S. aureus was 350.29 mm2.
33049811	5	13	contain	had	638:640	arg1	films					632:636	all the composite films	614:636	all the composite films	614:636	As for the antibacterial activity, all the composite films had strong antibacterial activity against Gram-negative (E. coli) and Gram-positive (S. aureus), and the inhibition zone of C/D/S1.5:1:0.10 against E. coli reached 551.96 mm2, the inhibition zone for S. aureus was 350.29 mm2.
33049811	8	14	contain	had	1119:1121	arg1	films					1113:1117	the composite films	1099:1117	the composite films	1099:1117	In summary, the composite films had excellent performance and structure, which promoted the research of functional packaging films.
33049811	8	14	contain	had	1119:1121	arg2	structure					1149:1157	structure	1149:1157	structure	1149:1157	In summary, the composite films had excellent performance and structure, which promoted the research of functional packaging films.
33049811	8	14	contain	had	1119:1121	arg2	performance					1133:1143	excellent performance	1123:1143	excellent performance	1123:1143	In summary, the composite films had excellent performance and structure, which promoted the research of functional packaging films.
33049811	0	15	theme	chitosan-	97:105	arg1	films					145:149	chitosan- nano-silicon aerogel composite edible films	97:149	chitosan- nano-silicon aerogel composite edible films	97:149	Investigation of the structural, physical properties, antioxidant, and antimicrobial activity of chitosan- nano-silicon aerogel composite edible films incorporated with okara powder.
33049811	4	16	theme	C/D/S1.5:1:0.1	547:560	arg1	film					562:565	C/D/S1.5:1:0.1 film	547:565	C/D/S1.5:1:0.1 film	547:565	The surface glossiness of C/D/S1.5:1:0.1 film was 14.4Gu.
33049811	5	17	theme	inhibition	818:827	arg1	350.29 mm2					852:861	350.29 mm2	852:861	350.29 mm2	852:861	As for the antibacterial activity, all the composite films had strong antibacterial activity against Gram-negative (E. coli) and Gram-positive (S. aureus), and the inhibition zone of C/D/S1.5:1:0.10 against E. coli reached 551.96 mm2, the inhibition zone for S. aureus was 350.29 mm2.
33049811	5	17	theme	inhibition	818:827	arg1	zone					829:832	the inhibition zone	814:832	the inhibition zone for S. aureus	814:846	As for the antibacterial activity, all the composite films had strong antibacterial activity against Gram-negative (E. coli) and Gram-positive (S. aureus), and the inhibition zone of C/D/S1.5:1:0.10 against E. coli reached 551.96 mm2, the inhibition zone for S. aureus was 350.29 mm2.
33049811	5	18	theme	composite	622:630	arg1	films					632:636	all the composite films	614:636	all the composite films	614:636	As for the antibacterial activity, all the composite films had strong antibacterial activity against Gram-negative (E. coli) and Gram-positive (S. aureus), and the inhibition zone of C/D/S1.5:1:0.10 against E. coli reached 551.96 mm2, the inhibition zone for S. aureus was 350.29 mm2.
33049811	2	19	theme	barrier	435:441	arg1	properties					443:452	barrier properties	435:452	barrier properties	435:452	The results showed that the composite film had good mechanical properties, barrier properties and optical properties.
33049811	6	20	theme	continuous	908:917	arg1	characteristics					937:951	uniform, non-porous, continuous and dense surface characteristics	887:951	uniform, non-porous, continuous and dense surface characteristics	887:951	The composite film had uniform, non-porous, continuous and dense surface characteristics.
33049811	6	21	theme	dense	923:927	arg1	characteristics					937:951	uniform, non-porous, continuous and dense surface characteristics	887:951	uniform, non-porous, continuous and dense surface characteristics	887:951	The composite film had uniform, non-porous, continuous and dense surface characteristics.
33049811	8	22	theme	functional	1191:1200	arg1	films					1212:1216	functional packaging films	1191:1216	functional packaging films	1191:1216	In summary, the composite films had excellent performance and structure, which promoted the research of functional packaging films.
33049811	4	23	theme	surface	525:531	arg1	glossiness					533:542	The surface glossiness	521:542	The surface glossiness of C/D/S1.5:1:0.1 film	521:565	The surface glossiness of C/D/S1.5:1:0.1 film was 14.4Gu.
33049811	1	24	theme	chitosan/okra	187:199	arg1	films					239:243	The chitosan/okra powder/nano-silicon aerogel composite films	183:243	The chitosan/okra powder/nano-silicon aerogel composite films	183:243	The chitosan/okra powder/nano-silicon aerogel composite films were prepared by casting method and their physicochemical properties and structural characterization were studied.
33049811	8	25	theme	films	1212:1216	arg1	research					1179:1186	the research	1175:1186	the research of functional packaging films	1175:1216	In summary, the composite films had excellent performance and structure, which promoted the research of functional packaging films.
33049811	1	26	theme	powder/nano-silicon	201:219	arg1	films					239:243	The chitosan/okra powder/nano-silicon aerogel composite films	183:243	The chitosan/okra powder/nano-silicon aerogel composite films	183:243	The chitosan/okra powder/nano-silicon aerogel composite films were prepared by casting method and their physicochemical properties and structural characterization were studied.
33049811	0	27	theme	structural	21:30	arg1	properties					42:51	physical properties	33:51	physical properties	33:51	Investigation of the structural, physical properties, antioxidant, and antimicrobial activity of chitosan- nano-silicon aerogel composite edible films incorporated with okara powder.
33049811	1	28	theme	structural	318:327	arg1	characterization					329:344	structural characterization	318:344	structural characterization	318:344	The chitosan/okra powder/nano-silicon aerogel composite films were prepared by casting method and their physicochemical properties and structural characterization were studied.
33049811	0	29	theme	composite	128:136	arg1	films					145:149	chitosan- nano-silicon aerogel composite edible films	97:149	chitosan- nano-silicon aerogel composite edible films	97:149	Investigation of the structural, physical properties, antioxidant, and antimicrobial activity of chitosan- nano-silicon aerogel composite edible films incorporated with okara powder.
33049811	5	30	theme	strong	642:647	arg1	activity					663:670	strong antibacterial activity	642:670	strong antibacterial activity against Gram-negative (E. coli) and Gram-positive (S. aureus)	642:732	As for the antibacterial activity, all the composite films had strong antibacterial activity against Gram-negative (E. coli) and Gram-positive (S. aureus), and the inhibition zone of C/D/S1.5:1:0.10 against E. coli reached 551.96 mm2, the inhibition zone for S. aureus was 350.29 mm2.
33049811	1	31	theme	aerogel	221:227	arg1	films					239:243	The chitosan/okra powder/nano-silicon aerogel composite films	183:243	The chitosan/okra powder/nano-silicon aerogel composite films	183:243	The chitosan/okra powder/nano-silicon aerogel composite films were prepared by casting method and their physicochemical properties and structural characterization were studied.
33049811	5	32	dep	Gram-positive	708:720	arg1	aureus					726:731	S. aureus	723:731	S. aureus	723:731	As for the antibacterial activity, all the composite films had strong antibacterial activity against Gram-negative (E. coli) and Gram-positive (S. aureus), and the inhibition zone of C/D/S1.5:1:0.10 against E. coli reached 551.96 mm2, the inhibition zone for S. aureus was 350.29 mm2.
33049811	0	33	theme	aerogel	120:126	arg1	films					145:149	chitosan- nano-silicon aerogel composite edible films	97:149	chitosan- nano-silicon aerogel composite edible films	97:149	Investigation of the structural, physical properties, antioxidant, and antimicrobial activity of chitosan- nano-silicon aerogel composite edible films incorporated with okara powder.
33049811	7	34	theme	good	1011:1014	arg1	compatibility					1016:1028	good compatibility	1011:1028	good compatibility between chitosan, okara powder and nano-silicon aerogel	1011:1084	The structural characterization confirmed that there was good compatibility between chitosan, okara powder and nano-silicon aerogel.
33049811	1	35	theme	composite	229:237	arg1	films					239:243	The chitosan/okra powder/nano-silicon aerogel composite films	183:243	The chitosan/okra powder/nano-silicon aerogel composite films	183:243	The chitosan/okra powder/nano-silicon aerogel composite films were prepared by casting method and their physicochemical properties and structural characterization were studied.
33049811	0	36	theme	physical	33:40	arg1	properties					42:51	physical properties	33:51	physical properties	33:51	Investigation of the structural, physical properties, antioxidant, and antimicrobial activity of chitosan- nano-silicon aerogel composite edible films incorporated with okara powder.
33049811	0	37	theme	films	145:149	arg1	antioxidant					54:64	antioxidant	54:64	antioxidant	54:64	Investigation of the structural, physical properties, antioxidant, and antimicrobial activity of chitosan- nano-silicon aerogel composite edible films incorporated with okara powder.
33049811	0	37	theme	films	145:149	arg1	properties					42:51	physical properties	33:51	physical properties	33:51	Investigation of the structural, physical properties, antioxidant, and antimicrobial activity of chitosan- nano-silicon aerogel composite edible films incorporated with okara powder.
33049811	0	37	theme	films	145:149	arg1	activity					85:92	antimicrobial activity	71:92	antimicrobial activity	71:92	Investigation of the structural, physical properties, antioxidant, and antimicrobial activity of chitosan- nano-silicon aerogel composite edible films incorporated with okara powder.
33049811	3	38	theme	strong	501:506	arg1	flexibility					508:518	strong flexibility	501:518	strong flexibility	501:518	The composite film has strong flexibility.
33049811	3	39	theme	composite	482:490	arg1	film					492:495	The composite film	478:495	The composite film	478:495	The composite film has strong flexibility.
33049811	2	40	theme	optical	458:464	arg1	properties					466:475	optical properties	458:475	optical properties	458:475	The results showed that the composite film had good mechanical properties, barrier properties and optical properties.
33049811	0	41	theme	edible	138:143	arg1	films					145:149	chitosan- nano-silicon aerogel composite edible films	97:149	chitosan- nano-silicon aerogel composite edible films	97:149	Investigation of the structural, physical properties, antioxidant, and antimicrobial activity of chitosan- nano-silicon aerogel composite edible films incorporated with okara powder.
33049811	8	42	theme	packaging	1202:1210	arg1	films					1212:1216	functional packaging films	1191:1216	functional packaging films	1191:1216	In summary, the composite films had excellent performance and structure, which promoted the research of functional packaging films.
33049811	0	43	dep	properties	42:51	arg1	the					17:19	the	17:19	the	17:19	Investigation of the structural, physical properties, antioxidant, and antimicrobial activity of chitosan- nano-silicon aerogel composite edible films incorporated with okara powder.
33049811	6	44	theme	uniform	887:893	arg1	characteristics					937:951	uniform, non-porous, continuous and dense surface characteristics	887:951	uniform, non-porous, continuous and dense surface characteristics	887:951	The composite film had uniform, non-porous, continuous and dense surface characteristics.
33049811	2	45	theme	composite	388:396	arg1	film					398:401	the composite film	384:401	the composite film	384:401	The results showed that the composite film had good mechanical properties, barrier properties and optical properties.
33049811	0	46	theme	properties	42:51	arg1	Investigation					0:12	Investigation	0:12	Investigation of the structural, physical properties, antioxidant, and antimicrobial activity of chitosan- nano-silicon aerogel composite edible films	0:149	Investigation of the structural, physical properties, antioxidant, and antimicrobial activity of chitosan- nano-silicon aerogel composite edible films incorporated with okara powder.
33049811	8	47	theme	composite	1103:1111	arg1	films					1113:1117	the composite films	1099:1117	the composite films	1099:1117	In summary, the composite films had excellent performance and structure, which promoted the research of functional packaging films.
33049811	7	48	theme	nano-silicon	1065:1076	arg1	aerogel					1078:1084	nano-silicon aerogel	1065:1084	nano-silicon aerogel	1065:1084	The structural characterization confirmed that there was good compatibility between chitosan, okara powder and nano-silicon aerogel.
33049811	5	49	dep	Gram-negative	680:692	arg1	coli					698:701	E. coli	695:701	E. coli	695:701	As for the antibacterial activity, all the composite films had strong antibacterial activity against Gram-negative (E. coli) and Gram-positive (S. aureus), and the inhibition zone of C/D/S1.5:1:0.10 against E. coli reached 551.96 mm2, the inhibition zone for S. aureus was 350.29 mm2.
33049811	0	50	theme	okara	169:173	arg1	powder					175:180	okara powder	169:180	okara powder	169:180	Investigation of the structural, physical properties, antioxidant, and antimicrobial activity of chitosan- nano-silicon aerogel composite edible films incorporated with okara powder.
33049811	3	51	contain	has	497:499	arg1	film					492:495	The composite film	478:495	The composite film	478:495	The composite film has strong flexibility.
33049811	3	51	contain	has	497:499	arg2	flexibility					508:518	strong flexibility	501:518	strong flexibility	501:518	The composite film has strong flexibility.
33049811	7	52	theme	okara	1048:1052	arg1	powder					1054:1059	okara powder	1048:1059	okara powder	1048:1059	The structural characterization confirmed that there was good compatibility between chitosan, okara powder and nano-silicon aerogel.
33049811	6	53	theme	non-porous	896:905	arg1	characteristics					937:951	uniform, non-porous, continuous and dense surface characteristics	887:951	uniform, non-porous, continuous and dense surface characteristics	887:951	The composite film had uniform, non-porous, continuous and dense surface characteristics.
33049811	2	54	theme	mechanical	412:421	arg1	properties					423:432	good mechanical properties	407:432	good mechanical properties	407:432	The results showed that the composite film had good mechanical properties, barrier properties and optical properties.
33049811	7	55	theme	structural	958:967	arg1	characterization					969:984	The structural characterization	954:984	The structural characterization	954:984	The structural characterization confirmed that there was good compatibility between chitosan, okara powder and nano-silicon aerogel.
33049811	1	56	theme	casting	262:268	arg1	method					270:275	casting method	262:275	casting method	262:275	The chitosan/okra powder/nano-silicon aerogel composite films were prepared by casting method and their physicochemical properties and structural characterization were studied.
33049811	0	57	theme	antioxidant	54:64	arg1	Investigation					0:12	Investigation	0:12	Investigation of the structural, physical properties, antioxidant, and antimicrobial activity of chitosan- nano-silicon aerogel composite edible films	0:149	Investigation of the structural, physical properties, antioxidant, and antimicrobial activity of chitosan- nano-silicon aerogel composite edible films incorporated with okara powder.
33049811	8	58	theme	excellent	1123:1131	arg1	performance					1133:1143	excellent performance	1123:1143	excellent performance	1123:1143	In summary, the composite films had excellent performance and structure, which promoted the research of functional packaging films.
33049811	2	59	theme	good	407:410	arg1	properties					423:432	good mechanical properties	407:432	good mechanical properties	407:432	The results showed that the composite film had good mechanical properties, barrier properties and optical properties.
33872940	4	0	dep	F.	691:692	arg1	graminearum					694:704	F. graminearum	691:704	F. graminearum	691:704	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	1	1	theme	Dextrose	235:242	arg1	medium					255:260	the popular Potato Dextrose Agar (PDA) medium	216:260	the popular Potato Dextrose Agar (PDA) medium	216:260	Potatoes contain several nutrients essential for fungal growth, making them an excellent component of media such as the popular Potato Dextrose Agar (PDA) medium.
33872940	0	2	theme	metabolite	65:74	arg1	production					76:85	secondary metabolite production	55:85	secondary metabolite production in Fusarium	55:97	The effects of different potato dextrose agar media on secondary metabolite production in Fusarium.
33872940	0	3	from	effects	4:10	arg1	production					76:85	secondary metabolite production	55:85	secondary metabolite production in Fusarium	55:97	The effects of different potato dextrose agar media on secondary metabolite production in Fusarium.
33872940	5	4	from	differences	837:847	arg1	intensities					852:862	intensities	852:862	intensities	852:862	Secondary metabolites were analysed using HPLC-HRMS, from which statistically significant differences in intensities were observed for 9 out of 10 metabolites.
33872940	4	5	theme	metabolite	629:638	arg1	profile					640:646	the metabolite profile	625:646	the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum)	625:744	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	1	6	theme	Agar	244:247	arg1	medium					255:260	the popular Potato Dextrose Agar (PDA) medium	216:260	the popular Potato Dextrose Agar (PDA) medium	216:260	Potatoes contain several nutrients essential for fungal growth, making them an excellent component of media such as the popular Potato Dextrose Agar (PDA) medium.
33872940	1	7	contain	contain	109:115	arg2	nutrients					125:133	several nutrients	117:133	several nutrients essential for fungal growth	117:161	Potatoes contain several nutrients essential for fungal growth, making them an excellent component of media such as the popular Potato Dextrose Agar (PDA) medium.
33872940	1	7	contain	contain	109:115	arg1	Potatoes					100:107	Potatoes	100:107	Potatoes	100:107	Potatoes contain several nutrients essential for fungal growth, making them an excellent component of media such as the popular Potato Dextrose Agar (PDA) medium.
33872940	5	8	theme	significant	825:835	arg1	differences					837:847	statistically significant differences	811:847	statistically significant differences in intensities	811:862	Secondary metabolites were analysed using HPLC-HRMS, from which statistically significant differences in intensities were observed for 9 out of 10 metabolites.
33872940	4	9	from	manufacturers	560:572	arg1	use					523:525	the use	519:525	the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum)	519:744	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	4	9	from	manufacturers	560:572	arg1	profile					640:646	the metabolite profile	625:646	the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum)	625:744	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	4	9	from	manufacturers	560:572	arg1	media					539:543	four PDA media	530:543	four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum)	530:744	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	3	10	contain	contain	383:389	arg1	media					361:365	These media	355:365	These media	355:365	These media, however, could contain small differences in composition of nutrients affecting the expression of secondary metabolites.
33872940	3	10	contain	contain	383:389	arg2	differences					397:407	small differences	391:407	small differences in composition of nutrients affecting the expression of secondary metabolites	391:485	These media, however, could contain small differences in composition of nutrients affecting the expression of secondary metabolites.
33872940	4	11	from	effect	615:620	arg1	use					523:525	the use	519:525	the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum)	519:744	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	4	11	from	effect	615:620	arg1	profile					640:646	the metabolite profile	625:646	the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum)	625:744	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	4	11	from	effect	615:620	arg1	media					539:543	four PDA media	530:543	four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum)	530:744	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	1	12	theme	PDA	250:252	arg1	medium					255:260	the popular Potato Dextrose Agar (PDA) medium	216:260	the popular Potato Dextrose Agar (PDA) medium	216:260	Potatoes contain several nutrients essential for fungal growth, making them an excellent component of media such as the popular Potato Dextrose Agar (PDA) medium.
33872940	2	13	from	retailers	308:316	arg1	available					284:292	available	284:292	available	284:292	Commercially, PDA is available from multiple retailers offering virtually the same product.
33872940	4	14	dep	F.	732:733	arg1	avenaceum					735:743	F. avenaceum	732:743	F. avenaceum	732:743	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	4	15	from	use	523:525	arg1	manufacturers					560:572	different manufacturers	550:572	different manufacturers (Fluka, Oxoid, Sigma, and VWR)	550:603	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	4	15	from	use	523:525	arg1	Sigma					589:593	Sigma	589:593	Sigma	589:593	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	4	15	from	use	523:525	arg1	VWR					600:602	VWR	600:602	VWR	600:602	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	4	15	from	use	523:525	arg1	Fluka					575:579	Fluka	575:579	Fluka	575:579	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	4	15	from	use	523:525	arg1	Oxoid					582:586	Oxoid	582:586	Oxoid	582:586	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	4	15	from	use	523:525	arg1	effect					615:620	their effect	609:620	their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum)	609:744	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	4	16	theme	different	550:558	arg1	manufacturers					560:572	different manufacturers	550:572	different manufacturers (Fluka, Oxoid, Sigma, and VWR)	550:603	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	4	16	theme	different	550:558	arg1	Sigma					589:593	Sigma	589:593	Sigma	589:593	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	4	16	theme	different	550:558	arg1	VWR					600:602	VWR	600:602	VWR	600:602	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	4	16	theme	different	550:558	arg1	Oxoid					582:586	Oxoid	582:586	Oxoid	582:586	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	4	16	theme	different	550:558	arg1	Fluka					575:579	Fluka	575:579	Fluka	575:579	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	4	17	theme	PDA	535:537	arg1	media					539:543	four PDA media	530:543	four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum)	530:744	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	3	18	theme	small	391:395	arg1	differences					397:407	small differences	391:407	small differences in composition of nutrients affecting the expression of secondary metabolites	391:485	These media, however, could contain small differences in composition of nutrients affecting the expression of secondary metabolites.
33872940	4	19	theme	species	656:662	arg1	profile					640:646	the metabolite profile	625:646	the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum)	625:744	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	4	20	theme	Fusarium	667:674	arg1	species					656:662	four species	651:662	four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum)	651:744	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	1	21	theme	excellent	179:187	arg1	component					189:197	an excellent component	176:197	them an excellent component of media such as the popular Potato Dextrose Agar (PDA) medium	171:260	Potatoes contain several nutrients essential for fungal growth, making them an excellent component of media such as the popular Potato Dextrose Agar (PDA) medium.
33872940	0	22	theme	different	15:23	arg1	media					46:50	different potato dextrose agar media	15:50	different potato dextrose agar media	15:50	The effects of different potato dextrose agar media on secondary metabolite production in Fusarium.
33872940	2	23	theme	same	341:344	arg1	product					346:352	virtually the same product	327:352	virtually the same product	327:352	Commercially, PDA is available from multiple retailers offering virtually the same product.
33872940	5	24	theme	Secondary	747:755	arg1	metabolites					757:767	Secondary metabolites	747:767	Secondary metabolites	747:767	Secondary metabolites were analysed using HPLC-HRMS, from which statistically significant differences in intensities were observed for 9 out of 10 metabolites.
33872940	4	25	theme	media	539:543	arg1	use					523:525	the use	519:525	the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum)	519:744	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	0	26	from	production	76:85	arg1	Fusarium					90:97	Fusarium	90:97	Fusarium	90:97	The effects of different potato dextrose agar media on secondary metabolite production in Fusarium.
33872940	0	27	theme	dextrose	32:39	arg1	media					46:50	different potato dextrose agar media	15:50	different potato dextrose agar media	15:50	The effects of different potato dextrose agar media on secondary metabolite production in Fusarium.
33872940	3	28	theme	metabolites	475:485	arg1	expression					451:460	the expression	447:460	the expression of secondary metabolites	447:485	These media, however, could contain small differences in composition of nutrients affecting the expression of secondary metabolites.
33872940	4	29	dep	manufacturers	560:572	arg1	manufacturers					560:572	different manufacturers	550:572	different manufacturers (Fluka, Oxoid, Sigma, and VWR)	550:603	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	4	29	dep	manufacturers	560:572	arg1	Sigma					589:593	Sigma	589:593	Sigma	589:593	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	4	29	dep	manufacturers	560:572	arg1	VWR					600:602	VWR	600:602	VWR	600:602	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	4	29	dep	manufacturers	560:572	arg1	Oxoid					582:586	Oxoid	582:586	Oxoid	582:586	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	4	29	dep	manufacturers	560:572	arg1	Fluka					575:579	Fluka	575:579	Fluka	575:579	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	4	30	dep	F.	707:708	arg1	pseudograminearum					710:726	F. pseudograminearum	707:726	F. pseudograminearum	707:726	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	1	31	theme	media	202:206	arg1	component					189:197	an excellent component	176:197	them an excellent component of media such as the popular Potato Dextrose Agar (PDA) medium	171:260	Potatoes contain several nutrients essential for fungal growth, making them an excellent component of media such as the popular Potato Dextrose Agar (PDA) medium.
33872940	0	32	theme	potato	25:30	arg1	media					46:50	different potato dextrose agar media	15:50	different potato dextrose agar media	15:50	The effects of different potato dextrose agar media on secondary metabolite production in Fusarium.
33872940	3	33	theme	nutrients	427:435	arg1	composition					412:422	composition	412:422	composition of nutrients affecting the expression of secondary metabolites	412:485	These media, however, could contain small differences in composition of nutrients affecting the expression of secondary metabolites.
33872940	0	34	theme	media	46:50	arg1	effects					4:10	The effects	0:10	The effects of different potato dextrose agar media on secondary metabolite production in Fusarium	0:97	The effects of different potato dextrose agar media on secondary metabolite production in Fusarium.
33872940	3	35	theme	secondary	465:473	arg1	metabolites					475:485	secondary metabolites	465:485	secondary metabolites	465:485	These media, however, could contain small differences in composition of nutrients affecting the expression of secondary metabolites.
33872940	0	36	theme	agar	41:44	arg1	media					46:50	different potato dextrose agar media	15:50	different potato dextrose agar media	15:50	The effects of different potato dextrose agar media on secondary metabolite production in Fusarium.
33872940	1	37	dep	them	171:174	arg1	component					189:197	an excellent component	176:197	them an excellent component of media such as the popular Potato Dextrose Agar (PDA) medium	171:260	Potatoes contain several nutrients essential for fungal growth, making them an excellent component of media such as the popular Potato Dextrose Agar (PDA) medium.
33872940	1	38	theme	several	117:123	arg1	nutrients					125:133	several nutrients	117:133	several nutrients essential for fungal growth	117:161	Potatoes contain several nutrients essential for fungal growth, making them an excellent component of media such as the popular Potato Dextrose Agar (PDA) medium.
33872940	2	39	theme	multiple	299:306	arg1	retailers					308:316	multiple retailers	299:316	multiple retailers offering virtually the same product	299:352	Commercially, PDA is available from multiple retailers offering virtually the same product.
33872940	1	40	theme	essential	135:143	arg1	nutrients					125:133	several nutrients	117:133	several nutrients essential for fungal growth	117:161	Potatoes contain several nutrients essential for fungal growth, making them an excellent component of media such as the popular Potato Dextrose Agar (PDA) medium.
33872940	0	41	theme	secondary	55:63	arg1	production					76:85	secondary metabolite production	55:85	secondary metabolite production in Fusarium	55:97	The effects of different potato dextrose agar media on secondary metabolite production in Fusarium.
33872940	3	42	from	differences	397:407	arg1	composition					412:422	composition	412:422	composition of nutrients affecting the expression of secondary metabolites	412:485	These media, however, could contain small differences in composition of nutrients affecting the expression of secondary metabolites.
33872940	1	43	theme	popular	220:226	arg1	medium					255:260	the popular Potato Dextrose Agar (PDA) medium	216:260	the popular Potato Dextrose Agar (PDA) medium	216:260	Potatoes contain several nutrients essential for fungal growth, making them an excellent component of media such as the popular Potato Dextrose Agar (PDA) medium.
33872940	4	44	dep	Fusarium	667:674	arg1	F.					677:678	F.	677:678	F.	677:678	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	4	44	dep	Fusarium	667:674	arg1	F.					691:692	F.	691:692	F.	691:692	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	4	44	dep	Fusarium	667:674	arg1	F.					707:708	F.	707:708	F.	707:708	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	4	44	dep	Fusarium	667:674	arg1	F.					732:733	F.	732:733	F.	732:733	This study aims to investigate the use of four PDA media from different manufacturers (Fluka, Oxoid, Sigma, and VWR) and their effect on the metabolite profile of four species of Fusarium (F. fujikuroi, F. graminearum, F. pseudograminearum and F. avenaceum).
33872940	1	45	theme	fungal	149:154	arg1	growth					156:161	fungal growth	149:161	fungal growth	149:161	Potatoes contain several nutrients essential for fungal growth, making them an excellent component of media such as the popular Potato Dextrose Agar (PDA) medium.
33872940	1	46	theme	Potato	228:233	arg1	medium					255:260	the popular Potato Dextrose Agar (PDA) medium	216:260	the popular Potato Dextrose Agar (PDA) medium	216:260	Potatoes contain several nutrients essential for fungal growth, making them an excellent component of media such as the popular Potato Dextrose Agar (PDA) medium.
32498383	0	0	theme	Long-Term	82:90	arg1	Diet					103:106	a Long-Term Low-FODMAP Diet	80:106	a Long-Term Low-FODMAP Diet	80:106	Improved Symptom Profiles and Minimal Inflammation in IBS-D Patients Undergoing a Long-Term Low-FODMAP Diet: A Lipidomic Perspective.
32498383	0	1	from	Profiles	17:24	arg1	Patients					60:67	IBS-D Patients	54:67	IBS-D Patients Undergoing a Long-Term Low-FODMAP Diet	54:106	Improved Symptom Profiles and Minimal Inflammation in IBS-D Patients Undergoing a Long-Term Low-FODMAP Diet: A Lipidomic Perspective.
32498383	1	2	from	oligosaccharides	311:326	arg1	low					292:294	low	292:294	low	292:294	Given the link between the minimal inflammation underlying irritable bowel syndrome (IBS) and dietary treatments, considerable attention has focused on diets low in fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs).
32498383	0	3	dep	Perspective	121:131	arg1	Profiles					17:24	Improved Symptom Profiles	0:24	Improved Symptom Profiles	0:24	Improved Symptom Profiles and Minimal Inflammation in IBS-D Patients Undergoing a Long-Term Low-FODMAP Diet: A Lipidomic Perspective.
32498383	0	3	dep	Perspective	121:131	arg1	Inflammation					38:49	Minimal Inflammation	30:49	Minimal Inflammation	30:49	Improved Symptom Profiles and Minimal Inflammation in IBS-D Patients Undergoing a Long-Term Low-FODMAP Diet: A Lipidomic Perspective.
32498383	2	4	theme	IBS	548:550	arg1	variant					570:576	the IBS diarrhoea (IBS-D) variant	544:576	the IBS diarrhoea (IBS-D) variant	544:576	In this context, inflammatory patterns and lipidomic investigations may shed light on the pathophysiological mechanisms whereby a low-FODMAP diet (LFD) improves the IBS diarrhoea (IBS-D) variant.
32498383	7	5	theme	AA/eicosapentaenoic	1228:1246	arg1	ratio					1253:1257	the AA/eicosapentaenoic acid ratio	1224:1257	the AA/eicosapentaenoic acid ratio	1224:1257	As for FAs, the putative inflammatory indicators, arachidonic acid (AA) levels and the AA/eicosapentaenoic acid ratio were significantly decreased.
32498383	1	6	theme	considerable	248:259	arg1	attention					261:269	considerable attention	248:269	considerable attention	248:269	Given the link between the minimal inflammation underlying irritable bowel syndrome (IBS) and dietary treatments, considerable attention has focused on diets low in fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs).
32498383	7	7	theme	acid	1203:1206	arg1	levels					1213:1218	arachidonic acid (AA) levels	1191:1218	arachidonic acid (AA) levels	1191:1218	As for FAs, the putative inflammatory indicators, arachidonic acid (AA) levels and the AA/eicosapentaenoic acid ratio were significantly decreased.
32498383	2	8	theme	pathophysiological	473:490	arg1	mechanisms					492:501	the pathophysiological mechanisms	469:501	the pathophysiological mechanisms whereby a low-FODMAP diet (LFD) improves the IBS diarrhoea (IBS-D) variant	469:576	In this context, inflammatory patterns and lipidomic investigations may shed light on the pathophysiological mechanisms whereby a low-FODMAP diet (LFD) improves the IBS diarrhoea (IBS-D) variant.
32498383	9	9	theme	Lipidomic	1444:1452	arg1	data					1454:1457	Lipidomic data	1444:1457	Lipidomic data	1444:1457	Lipidomic data may be insightful for unravelling the molecular mechanisms associated with IBS-D pathophysiology.
32498383	0	10	theme	Low-FODMAP	92:101	arg1	Diet					103:106	a Long-Term Low-FODMAP Diet	80:106	a Long-Term Low-FODMAP Diet	80:106	Improved Symptom Profiles and Minimal Inflammation in IBS-D Patients Undergoing a Long-Term Low-FODMAP Diet: A Lipidomic Perspective.
32498383	8	11	theme	long-term	1342:1350	arg1	LFD					1352:1354	a controlled long-term LFD	1329:1354	a controlled long-term LFD	1329:1354	In conclusion, IBS-D patients following a controlled long-term LFD experienced improved symptom profiles and decreased inflammatory markers linked to FAs.
32498383	6	12	from	decrease	1077:1084	arg1	E2					1103:1104	prostaglandin E2	1089:1104	prostaglandin E2	1089:1104	A significant decrease in prostaglandin E2 also accompanied these reductions.
32498383	6	13	theme	prostaglandin	1089:1101	arg1	E2					1103:1104	prostaglandin E2	1089:1104	prostaglandin E2	1089:1104	A significant decrease in prostaglandin E2 also accompanied these reductions.
32498383	3	14	from	changes	633:639	arg1	patients					838:845	IBS-D patients	832:845	IBS-D patients	832:845	Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients.
32498383	3	14	from	changes	633:639	arg1	protein					727:733	C-reactive protein	716:733	C-reactive protein	716:733	Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients.
32498383	3	14	from	changes	633:639	arg1	E2					772:773	prostaglandin E2	758:773	prostaglandin E2	758:773	Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients.
32498383	3	14	from	changes	633:639	arg1	characteristics					677:691	anthropometric characteristics	662:691	anthropometric characteristics	662:691	Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients.
32498383	3	14	from	changes	633:639	arg1	composition					817:827	erythrocyte-membrane fatty acid (FA) composition	780:827	erythrocyte-membrane fatty acid (FA) composition	780:827	Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients.
32498383	3	14	from	changes	633:639	arg1	cyclooxygenase-2					736:751	cyclooxygenase-2	736:751	cyclooxygenase-2	736:751	Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients.
32498383	3	14	from	changes	633:639	arg1	markers					707:713	inflammatory markers	694:713	inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2)	694:774	Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients.
32498383	3	14	from	changes	633:639	arg1	profiles					652:659	symptom profiles	644:659	symptom profiles	644:659	Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients.
32498383	1	15	theme	minimal	161:167	arg1	inflammation					169:180	the minimal inflammation	157:180	the minimal inflammation underlying irritable bowel syndrome (IBS)	157:222	Given the link between the minimal inflammation underlying irritable bowel syndrome (IBS) and dietary treatments, considerable attention has focused on diets low in fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs).
32498383	4	16	theme	scheduled	949:957	arg1	visits					959:964	scheduled visits	949:964	scheduled visits	949:964	Twenty IBS-D patients underwent a 90 day personalised LFD programme, and were regularly evaluated at scheduled visits.
32498383	0	17	from	Inflammation	38:49	arg1	Patients					60:67	IBS-D Patients	54:67	IBS-D Patients Undergoing a Long-Term Low-FODMAP Diet	54:106	Improved Symptom Profiles and Minimal Inflammation in IBS-D Patients Undergoing a Long-Term Low-FODMAP Diet: A Lipidomic Perspective.
32498383	1	18	from	low	292:294	arg1	monosaccharides					344:358	monosaccharides	344:358	monosaccharides	344:358	Given the link between the minimal inflammation underlying irritable bowel syndrome (IBS) and dietary treatments, considerable attention has focused on diets low in fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs).
32498383	1	18	from	low	292:294	arg1	FODMAPs					373:379	FODMAPs	373:379	FODMAPs	373:379	Given the link between the minimal inflammation underlying irritable bowel syndrome (IBS) and dietary treatments, considerable attention has focused on diets low in fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs).
32498383	1	18	from	low	292:294	arg1	disaccharides					329:341	disaccharides	329:341	disaccharides	329:341	Given the link between the minimal inflammation underlying irritable bowel syndrome (IBS) and dietary treatments, considerable attention has focused on diets low in fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs).
32498383	1	18	from	low	292:294	arg1	oligosaccharides					311:326	fermentable oligosaccharides	299:326	fermentable oligosaccharides	299:326	Given the link between the minimal inflammation underlying irritable bowel syndrome (IBS) and dietary treatments, considerable attention has focused on diets low in fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs).
32498383	1	18	from	low	292:294	arg1	polyols					364:370	polyols	364:370	polyols	364:370	Given the link between the minimal inflammation underlying irritable bowel syndrome (IBS) and dietary treatments, considerable attention has focused on diets low in fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs).
32498383	6	19	theme	significant	1065:1075	arg1	decrease					1077:1084	A significant decrease	1063:1084	A significant decrease in prostaglandin E2	1063:1104	A significant decrease in prostaglandin E2 also accompanied these reductions.
32498383	0	20	theme	Symptom	9:15	arg1	Profiles					17:24	Improved Symptom Profiles	0:24	Improved Symptom Profiles	0:24	Improved Symptom Profiles and Minimal Inflammation in IBS-D Patients Undergoing a Long-Term Low-FODMAP Diet: A Lipidomic Perspective.
32498383	3	21	theme	C-reactive	716:725	arg1	markers					707:713	inflammatory markers	694:713	inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2)	694:774	Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients.
32498383	3	21	theme	C-reactive	716:725	arg1	protein					727:733	C-reactive protein	716:733	C-reactive protein	716:733	Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients.
32498383	4	22	theme	IBS-D	855:859	arg1	patients					861:868	Twenty IBS-D patients	848:868	Twenty IBS-D patients	848:868	Twenty IBS-D patients underwent a 90 day personalised LFD programme, and were regularly evaluated at scheduled visits.
32498383	0	23	theme	Improved	0:7	arg1	Profiles					17:24	Improved Symptom Profiles	0:24	Improved Symptom Profiles	0:24	Improved Symptom Profiles and Minimal Inflammation in IBS-D Patients Undergoing a Long-Term Low-FODMAP Diet: A Lipidomic Perspective.
32498383	8	24	theme	symptom	1377:1383	arg1	profiles					1385:1392	symptom profiles	1377:1392	symptom profiles	1377:1392	In conclusion, IBS-D patients following a controlled long-term LFD experienced improved symptom profiles and decreased inflammatory markers linked to FAs.
32498383	3	25	theme	erythrocyte-membrane	780:799	arg1	FA					813:814	FA	813:814	FA	813:814	Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients.
32498383	3	25	theme	erythrocyte-membrane	780:799	arg1	acid					807:810	erythrocyte-membrane fatty acid	780:810	erythrocyte-membrane fatty acid (FA) composition	780:827	Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients.
32498383	1	26	theme	irritable	193:201	arg1	syndrome					209:216	irritable bowel syndrome	193:216	irritable bowel syndrome (IBS)	193:222	Given the link between the minimal inflammation underlying irritable bowel syndrome (IBS) and dietary treatments, considerable attention has focused on diets low in fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs).
32498383	1	26	theme	irritable	193:201	arg1	IBS					219:221	IBS	219:221	IBS	219:221	Given the link between the minimal inflammation underlying irritable bowel syndrome (IBS) and dietary treatments, considerable attention has focused on diets low in fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs).
32498383	1	27	from	polyols	364:370	arg1	low					292:294	low	292:294	low	292:294	Given the link between the minimal inflammation underlying irritable bowel syndrome (IBS) and dietary treatments, considerable attention has focused on diets low in fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs).
32498383	3	28	theme	prostaglandin	758:770	arg1	markers					707:713	inflammatory markers	694:713	inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2)	694:774	Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients.
32498383	3	28	theme	prostaglandin	758:770	arg1	E2					772:773	prostaglandin E2	758:773	prostaglandin E2	758:773	Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients.
32498383	2	29	theme	diarrhoea	552:560	arg1	variant					570:576	the IBS diarrhoea (IBS-D) variant	544:576	the IBS diarrhoea (IBS-D) variant	544:576	In this context, inflammatory patterns and lipidomic investigations may shed light on the pathophysiological mechanisms whereby a low-FODMAP diet (LFD) improves the IBS diarrhoea (IBS-D) variant.
32498383	1	30	theme	bowel	203:207	arg1	syndrome					209:216	irritable bowel syndrome	193:216	irritable bowel syndrome (IBS)	193:222	Given the link between the minimal inflammation underlying irritable bowel syndrome (IBS) and dietary treatments, considerable attention has focused on diets low in fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs).
32498383	1	30	theme	bowel	203:207	arg1	IBS					219:221	IBS	219:221	IBS	219:221	Given the link between the minimal inflammation underlying irritable bowel syndrome (IBS) and dietary treatments, considerable attention has focused on diets low in fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs).
32498383	2	31	theme	lipidomic	426:434	arg1	investigations					436:449	lipidomic investigations	426:449	lipidomic investigations	426:449	In this context, inflammatory patterns and lipidomic investigations may shed light on the pathophysiological mechanisms whereby a low-FODMAP diet (LFD) improves the IBS diarrhoea (IBS-D) variant.
32498383	0	32	theme	Lipidomic	111:119	arg1	Perspective					121:131	A Lipidomic Perspective	109:131	A Lipidomic Perspective	109:131	Improved Symptom Profiles and Minimal Inflammation in IBS-D Patients Undergoing a Long-Term Low-FODMAP Diet: A Lipidomic Perspective.
32498383	8	33	attach	linked	1429:1434	arg1	FAs					1439:1441	FAs	1439:1441	FAs	1439:1441	In conclusion, IBS-D patients following a controlled long-term LFD experienced improved symptom profiles and decreased inflammatory markers linked to FAs.
32498383	8	33	attach	linked	1429:1434	arg2	markers					1421:1427	inflammatory markers	1408:1427	inflammatory markers linked to FAs	1408:1441	In conclusion, IBS-D patients following a controlled long-term LFD experienced improved symptom profiles and decreased inflammatory markers linked to FAs.
32498383	1	34	from	monosaccharides	344:358	arg1	low					292:294	low	292:294	low	292:294	Given the link between the minimal inflammation underlying irritable bowel syndrome (IBS) and dietary treatments, considerable attention has focused on diets low in fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs).
32498383	1	35	theme	low	292:294	arg1	diets					286:290	diets	286:290	diets low in fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs)	286:380	Given the link between the minimal inflammation underlying irritable bowel syndrome (IBS) and dietary treatments, considerable attention has focused on diets low in fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs).
32498383	9	36	theme	molecular	1497:1505	arg1	mechanisms					1507:1516	the molecular mechanisms	1493:1516	the molecular mechanisms associated with IBS-D pathophysiology	1493:1554	Lipidomic data may be insightful for unravelling the molecular mechanisms associated with IBS-D pathophysiology.
32498383	5	37	theme	IBS	991:993	arg1	symptoms					995:1002	both IBS symptoms	986:1002	both IBS symptoms	986:1002	At the diet's end, both IBS symptoms and anthropometric parameters were significantly improved.
32498383	2	38	theme	inflammatory	400:411	arg1	patterns					413:420	inflammatory patterns	400:420	inflammatory patterns	400:420	In this context, inflammatory patterns and lipidomic investigations may shed light on the pathophysiological mechanisms whereby a low-FODMAP diet (LFD) improves the IBS diarrhoea (IBS-D) variant.
32498383	0	39	theme	Minimal	30:36	arg1	Inflammation					38:49	Minimal Inflammation	30:49	Minimal Inflammation	30:49	Improved Symptom Profiles and Minimal Inflammation in IBS-D Patients Undergoing a Long-Term Low-FODMAP Diet: A Lipidomic Perspective.
32498383	3	40	theme	IBS-D	832:836	arg1	patients					838:845	IBS-D patients	832:845	IBS-D patients	832:845	Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients.
32498383	3	41	theme	fatty	801:805	arg1	FA					813:814	FA	813:814	FA	813:814	Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients.
32498383	3	41	theme	fatty	801:805	arg1	acid					807:810	erythrocyte-membrane fatty acid	780:810	erythrocyte-membrane fatty acid (FA) composition	780:827	Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients.
32498383	7	42	theme	arachidonic	1191:1201	arg1	acid					1203:1206	arachidonic acid	1191:1206	arachidonic acid (AA) levels	1191:1218	As for FAs, the putative inflammatory indicators, arachidonic acid (AA) levels and the AA/eicosapentaenoic acid ratio were significantly decreased.
32498383	7	42	theme	arachidonic	1191:1201	arg1	AA					1209:1210	AA	1209:1210	AA	1209:1210	As for FAs, the putative inflammatory indicators, arachidonic acid (AA) levels and the AA/eicosapentaenoic acid ratio were significantly decreased.
32498383	0	43	theme	IBS-D	54:58	arg1	Patients					60:67	IBS-D Patients	54:67	IBS-D Patients Undergoing a Long-Term Low-FODMAP Diet	54:106	Improved Symptom Profiles and Minimal Inflammation in IBS-D Patients Undergoing a Long-Term Low-FODMAP Diet: A Lipidomic Perspective.
32498383	1	44	theme	fermentable	299:309	arg1	FODMAPs					373:379	FODMAPs	373:379	FODMAPs	373:379	Given the link between the minimal inflammation underlying irritable bowel syndrome (IBS) and dietary treatments, considerable attention has focused on diets low in fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs).
32498383	1	44	theme	fermentable	299:309	arg1	oligosaccharides					311:326	fermentable oligosaccharides	299:326	fermentable oligosaccharides	299:326	Given the link between the minimal inflammation underlying irritable bowel syndrome (IBS) and dietary treatments, considerable attention has focused on diets low in fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs).
32498383	4	45	theme	personalised	889:900	arg1	programme					906:914	a 90 day personalised LFD programme	880:914	a 90 day personalised LFD programme	880:914	Twenty IBS-D patients underwent a 90 day personalised LFD programme, and were regularly evaluated at scheduled visits.
32498383	4	46	theme	day	885:887	arg1	programme					906:914	a 90 day personalised LFD programme	880:914	a 90 day personalised LFD programme	880:914	Twenty IBS-D patients underwent a 90 day personalised LFD programme, and were regularly evaluated at scheduled visits.
32498383	5	47	theme	anthropometric	1008:1021	arg1	parameters					1023:1032	anthropometric parameters	1008:1032	anthropometric parameters	1008:1032	At the diet's end, both IBS symptoms and anthropometric parameters were significantly improved.
32498383	2	48	theme	IBS-D	563:567	arg1	variant					570:576	the IBS diarrhoea (IBS-D) variant	544:576	the IBS diarrhoea (IBS-D) variant	544:576	In this context, inflammatory patterns and lipidomic investigations may shed light on the pathophysiological mechanisms whereby a low-FODMAP diet (LFD) improves the IBS diarrhoea (IBS-D) variant.
32498383	3	49	theme	anthropometric	662:675	arg1	characteristics					677:691	anthropometric characteristics	662:691	anthropometric characteristics	662:691	Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients.
32498383	3	50	dep	markers	707:713	arg1	cyclooxygenase-2					736:751	cyclooxygenase-2	736:751	cyclooxygenase-2	736:751	Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients.
32498383	3	50	dep	markers	707:713	arg1	markers					707:713	inflammatory markers	694:713	inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2)	694:774	Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients.
32498383	3	50	dep	markers	707:713	arg1	protein					727:733	C-reactive protein	716:733	C-reactive protein	716:733	Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients.
32498383	3	50	dep	markers	707:713	arg1	E2					772:773	prostaglandin E2	758:773	prostaglandin E2	758:773	Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients.
32498383	8	51	theme	inflammatory	1408:1419	arg1	markers					1421:1427	inflammatory markers	1408:1427	inflammatory markers linked to FAs	1408:1441	In conclusion, IBS-D patients following a controlled long-term LFD experienced improved symptom profiles and decreased inflammatory markers linked to FAs.
32498383	8	52	theme	IBS-D	1304:1308	arg1	patients					1310:1317	IBS-D patients	1304:1317	IBS-D patients following a controlled long-term LFD experienced	1304:1366	In conclusion, IBS-D patients following a controlled long-term LFD experienced improved symptom profiles and decreased inflammatory markers linked to FAs.
32498383	4	53	theme	LFD	902:904	arg1	programme					906:914	a 90 day personalised LFD programme	880:914	a 90 day personalised LFD programme	880:914	Twenty IBS-D patients underwent a 90 day personalised LFD programme, and were regularly evaluated at scheduled visits.
32498383	3	54	theme	symptom	644:650	arg1	profiles					652:659	symptom profiles	644:659	symptom profiles	644:659	Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients.
32498383	3	55	theme	long-term	611:619	arg1	LFD					621:623	a long-term LFD	609:623	a long-term LFD	609:623	Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients.
32498383	8	56	theme	controlled	1331:1340	arg1	LFD					1352:1354	a controlled long-term LFD	1329:1354	a controlled long-term LFD	1329:1354	In conclusion, IBS-D patients following a controlled long-term LFD experienced improved symptom profiles and decreased inflammatory markers linked to FAs.
32498383	9	57	theme	IBS-D	1534:1538	arg1	pathophysiology					1540:1554	IBS-D pathophysiology	1534:1554	IBS-D pathophysiology	1534:1554	Lipidomic data may be insightful for unravelling the molecular mechanisms associated with IBS-D pathophysiology.
32498383	2	58	theme	low-FODMAP	513:522	arg1	LFD					530:532	LFD	530:532	LFD	530:532	In this context, inflammatory patterns and lipidomic investigations may shed light on the pathophysiological mechanisms whereby a low-FODMAP diet (LFD) improves the IBS diarrhoea (IBS-D) variant.
32498383	2	58	theme	low-FODMAP	513:522	arg1	diet					524:527	a low-FODMAP diet	511:527	a low-FODMAP diet (LFD)	511:533	In this context, inflammatory patterns and lipidomic investigations may shed light on the pathophysiological mechanisms whereby a low-FODMAP diet (LFD) improves the IBS diarrhoea (IBS-D) variant.
32498383	7	59	theme	acid	1248:1251	arg1	ratio					1253:1257	the AA/eicosapentaenoic acid ratio	1224:1257	the AA/eicosapentaenoic acid ratio	1224:1257	As for FAs, the putative inflammatory indicators, arachidonic acid (AA) levels and the AA/eicosapentaenoic acid ratio were significantly decreased.
32498383	1	60	theme	dietary	228:234	arg1	treatments					236:245	dietary treatments	228:245	dietary treatments	228:245	Given the link between the minimal inflammation underlying irritable bowel syndrome (IBS) and dietary treatments, considerable attention has focused on diets low in fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs).
32498383	1	61	from	disaccharides	329:341	arg1	low					292:294	low	292:294	low	292:294	Given the link between the minimal inflammation underlying irritable bowel syndrome (IBS) and dietary treatments, considerable attention has focused on diets low in fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs).
32498383	3	62	theme	inflammatory	694:705	arg1	cyclooxygenase-2					736:751	cyclooxygenase-2	736:751	cyclooxygenase-2	736:751	Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients.
32498383	3	62	theme	inflammatory	694:705	arg1	markers					707:713	inflammatory markers	694:713	inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2)	694:774	Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients.
32498383	3	62	theme	inflammatory	694:705	arg1	protein					727:733	C-reactive protein	716:733	C-reactive protein	716:733	Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients.
32498383	3	62	theme	inflammatory	694:705	arg1	E2					772:773	prostaglandin E2	758:773	prostaglandin E2	758:773	Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients.
32498383	7	63	theme	putative	1157:1164	arg1	indicators					1179:1188	the putative inflammatory indicators	1153:1188	the putative inflammatory indicators	1153:1188	As for FAs, the putative inflammatory indicators, arachidonic acid (AA) levels and the AA/eicosapentaenoic acid ratio were significantly decreased.
32498383	7	64	theme	inflammatory	1166:1177	arg1	indicators					1179:1188	the putative inflammatory indicators	1153:1188	the putative inflammatory indicators	1153:1188	As for FAs, the putative inflammatory indicators, arachidonic acid (AA) levels and the AA/eicosapentaenoic acid ratio were significantly decreased.
32498383	3	65	theme	acid	807:810	arg1	composition					817:827	erythrocyte-membrane fatty acid (FA) composition	780:827	erythrocyte-membrane fatty acid (FA) composition	780:827	Thus, we investigated whether a long-term LFD induced changes in symptom profiles, anthropometric characteristics, inflammatory markers (C-reactive protein, cyclooxygenase-2, and prostaglandin E2) and erythrocyte-membrane fatty acid (FA) composition in IBS-D patients.
32786859	2	0	theme	effluents	374:382	arg1	case					328:331	One such contamination case	305:331	One such contamination case	305:331	One such contamination case is the environmental disposal of colored effluents from the food processing industry (i.e., food dyes).
32786859	2	0	theme	effluents	374:382	arg1	disposal					354:361	the environmental disposal	336:361	the environmental disposal of colored effluents from the food processing industry (i.e., food dyes)	336:434	One such contamination case is the environmental disposal of colored effluents from the food processing industry (i.e., food dyes).
32786859	9	1	theme	other	1754:1758	arg1	materials					1790:1798	other polysaccharide-based hydrogel materials	1754:1798	other polysaccharide-based hydrogel materials	1754:1798	The facile, noncytotoxic, and general strategy presented here could be extended to the preparation of other polysaccharide-based hydrogel materials and has good prospects for application in wastewater treatment.
32786859	6	2	theme	thermogravimetric	1230:1246	arg1	analysis					1248:1255	thermogravimetric analysis	1230:1255	thermogravimetric analysis	1230:1255	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	9	3	theme	general	1682:1688	arg1	strategy					1690:1697	The facile, noncytotoxic, and general strategy	1652:1697	The facile, noncytotoxic, and general strategy presented here	1652:1712	The facile, noncytotoxic, and general strategy presented here could be extended to the preparation of other polysaccharide-based hydrogel materials and has good prospects for application in wastewater treatment.
32786859	6	4	theme	network	1307:1313	arg1	structure					1315:1323	a gel network structure	1301:1323	a gel network structure	1301:1323	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	1	5	theme	recent	291:296	arg1	years					298:302	recent years	291:302	recent years	291:302	Agricultural production-caused water contamination has become an urgent environmental issue that has drawn much attention in recent years.
32786859	9	6	theme	hydrogel	1781:1788	arg1	materials					1790:1798	other polysaccharide-based hydrogel materials	1754:1798	other polysaccharide-based hydrogel materials	1754:1798	The facile, noncytotoxic, and general strategy presented here could be extended to the preparation of other polysaccharide-based hydrogel materials and has good prospects for application in wastewater treatment.
32786859	3	7	attach	removing	459:466	arg1	water					490:494	water	490:494	water	490:494	Effective methods for removing dye contaminants from water have been increasingly sought, and different adsorbents have been developed for this purpose.
32786859	3	7	attach	removing	459:466	arg2	methods					447:453	Effective methods	437:453	Effective methods for removing dye contaminants from water	437:494	Effective methods for removing dye contaminants from water have been increasingly sought, and different adsorbents have been developed for this purpose.
32786859	1	8	theme	water	197:201	arg1	contamination					203:215	Agricultural production-caused water contamination	166:215	Agricultural production-caused water contamination	166:215	Agricultural production-caused water contamination has become an urgent environmental issue that has drawn much attention in recent years.
32786859	7	9	theme	synergistic	1466:1476	arg1	effects					1478:1484	their synergistic effects	1460:1484	their synergistic effects of absorption and photocatalytic degradation	1460:1529	As expected, the polysaccharide-based hydrogels exhibited good MB removal performance because of their synergistic effects of absorption and photocatalytic degradation.
32786859	8	10	contain	possessed	1619:1627	arg2	biocompatibility					1634:1649	good biocompatibility	1629:1649	good biocompatibility	1629:1649	Furthermore, the cell cytotoxicity test showed that the polysaccharide-based hydrogels possessed good biocompatibility.
32786859	8	10	contain	possessed	1619:1627	arg1	hydrogels					1609:1617	the polysaccharide-based hydrogels	1584:1617	the polysaccharide-based hydrogels	1584:1617	Furthermore, the cell cytotoxicity test showed that the polysaccharide-based hydrogels possessed good biocompatibility.
32786859	4	11	theme	methylene	694:702	arg1	removal					683:689	the removal	679:689	the removal of methylene blue (MB) (as the representative dye) from an aqueous medium (as simulated dye liquor wastewater)	679:800	Here, polysaccharide-based hydrogels derived from cellulose were constructed and used in the removal of methylene blue (MB) (as the representative dye) from an aqueous medium (as simulated dye liquor wastewater).
32786859	9	12	theme	good	1808:1811	arg1	prospects					1813:1821	good prospects	1808:1821	good prospects for application in wastewater treatment	1808:1861	The facile, noncytotoxic, and general strategy presented here could be extended to the preparation of other polysaccharide-based hydrogel materials and has good prospects for application in wastewater treatment.
32786859	5	13	with	hydrogels	942:950	arg1	advantages					968:977	respective advantages	957:977	respective advantages	957:977	To improve the purification efficiency, TiO2 nanoparticles were encapsulated into cellulose nanofibers, which were consequently changed to hydrogels with respective advantages.
32786859	0	14	theme	Dual	123:126	arg1	Function					128:135	a Putative Dual Function	112:135	a Putative Dual Function in Dye Wastewater Treatment	112:163	Polysaccharide-Based Hydrogels Derived from Cellulose: The Architecture Change from Nanofibers to Hydrogels for a Putative Dual Function in Dye Wastewater Treatment.
32786859	2	15	theme	contamination	314:326	arg1	case					328:331	One such contamination case	305:331	One such contamination case	305:331	One such contamination case is the environmental disposal of colored effluents from the food processing industry (i.e., food dyes).
32786859	2	15	theme	contamination	314:326	arg1	disposal					354:361	the environmental disposal	336:361	the environmental disposal of colored effluents from the food processing industry (i.e., food dyes)	336:434	One such contamination case is the environmental disposal of colored effluents from the food processing industry (i.e., food dyes).
32786859	5	16	theme	purification	818:829	arg1	efficiency					831:840	the purification efficiency	814:840	the purification efficiency	814:840	To improve the purification efficiency, TiO2 nanoparticles were encapsulated into cellulose nanofibers, which were consequently changed to hydrogels with respective advantages.
32786859	0	17	from	Nanofibers	84:93	arg1	Change					72:77	The Architecture Change	55:77	The Architecture Change from Nanofibers to Hydrogels for a Putative Dual Function in Dye Wastewater Treatment	55:163	Polysaccharide-Based Hydrogels Derived from Cellulose: The Architecture Change from Nanofibers to Hydrogels for a Putative Dual Function in Dye Wastewater Treatment.
32786859	3	18	theme	Effective	437:445	arg1	methods					447:453	Effective methods	437:453	Effective methods for removing dye contaminants from water	437:494	Effective methods for removing dye contaminants from water have been increasingly sought, and different adsorbents have been developed for this purpose.
32786859	1	19	theme	environmental	238:250	arg1	issue					252:256	an urgent environmental issue	228:256	an urgent environmental issue that has drawn much attention in recent years	228:302	Agricultural production-caused water contamination has become an urgent environmental issue that has drawn much attention in recent years.
32786859	7	20	theme	MB	1426:1427	arg1	performance					1437:1447	good MB removal performance	1421:1447	good MB removal performance	1421:1447	As expected, the polysaccharide-based hydrogels exhibited good MB removal performance because of their synergistic effects of absorption and photocatalytic degradation.
32786859	0	21	theme	Wastewater	144:153	arg1	Treatment					155:163	Dye Wastewater Treatment	140:163	Dye Wastewater Treatment	140:163	Polysaccharide-Based Hydrogels Derived from Cellulose: The Architecture Change from Nanofibers to Hydrogels for a Putative Dual Function in Dye Wastewater Treatment.
32786859	6	22	theme	X-ray	1262:1266	arg1	diffraction					1268:1278	X-ray diffraction	1262:1278	X-ray diffraction	1262:1278	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	4	23	theme	representative	722:735	arg1	dye					737:739	the representative dye	718:739	the representative dye	718:739	Here, polysaccharide-based hydrogels derived from cellulose were constructed and used in the removal of methylene blue (MB) (as the representative dye) from an aqueous medium (as simulated dye liquor wastewater).
32786859	6	24	theme	nanoparticles	1334:1346	arg1	presence					1289:1296	the presence	1285:1296	the presence of a gel network structure and TiO2 nanoparticles	1285:1346	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	4	25	used	used	671:674	arg2	hydrogels					617:625	polysaccharide-based hydrogels	596:625	polysaccharide-based hydrogels derived from cellulose	596:648	Here, polysaccharide-based hydrogels derived from cellulose were constructed and used in the removal of methylene blue (MB) (as the representative dye) from an aqueous medium (as simulated dye liquor wastewater).
32786859	8	26	theme	cytotoxicity	1554:1565	arg1	test					1567:1570	the cell cytotoxicity test	1545:1570	the cell cytotoxicity test	1545:1570	Furthermore, the cell cytotoxicity test showed that the polysaccharide-based hydrogels possessed good biocompatibility.
32786859	7	27	theme	polysaccharide-based	1380:1399	arg1	hydrogels					1401:1409	the polysaccharide-based hydrogels	1376:1409	the polysaccharide-based hydrogels	1376:1409	As expected, the polysaccharide-based hydrogels exhibited good MB removal performance because of their synergistic effects of absorption and photocatalytic degradation.
32786859	5	28	theme	cellulose	885:893	arg1	nanofibers					895:904	cellulose nanofibers	885:904	cellulose nanofibers	885:904	To improve the purification efficiency, TiO2 nanoparticles were encapsulated into cellulose nanofibers, which were consequently changed to hydrogels with respective advantages.
32786859	6	29	from	structure	1022:1030	arg1	nanofibers					1118:1127	nanofibers	1118:1127	nanofibers to hydrogels	1118:1140	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	1	30	theme	Agricultural	166:177	arg1	contamination					203:215	Agricultural production-caused water contamination	166:215	Agricultural production-caused water contamination	166:215	Agricultural production-caused water contamination has become an urgent environmental issue that has drawn much attention in recent years.
32786859	0	31	dep	Change	72:77	arg1	Hydrogels					21:29	Polysaccharide-Based Hydrogels	0:29	Polysaccharide-Based Hydrogels Derived from Cellulose	0:52	Polysaccharide-Based Hydrogels Derived from Cellulose: The Architecture Change from Nanofibers to Hydrogels for a Putative Dual Function in Dye Wastewater Treatment.
32786859	4	32	from	medium	758:763	arg1	removal					683:689	the removal	679:689	the removal of methylene blue (MB) (as the representative dye) from an aqueous medium (as simulated dye liquor wastewater)	679:800	Here, polysaccharide-based hydrogels derived from cellulose were constructed and used in the removal of methylene blue (MB) (as the representative dye) from an aqueous medium (as simulated dye liquor wastewater).
32786859	9	33	from	prospects	1813:1821	arg1	treatment					1853:1861	wastewater treatment	1842:1861	wastewater treatment	1842:1861	The facile, noncytotoxic, and general strategy presented here could be extended to the preparation of other polysaccharide-based hydrogel materials and has good prospects for application in wastewater treatment.
32786859	6	34	theme	process	1105:1111	arg1	composition					1005:1015	chemical composition	996:1015	chemical composition	996:1015	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	6	34	theme	process	1105:1111	arg1	structure					1022:1030	structure	1022:1030	structure	1022:1030	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	6	34	theme	process	1105:1111	arg1	morphology					984:993	The morphology	980:993	The morphology	980:993	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	8	35	theme	good	1629:1632	arg1	biocompatibility					1634:1649	good biocompatibility	1629:1649	good biocompatibility	1629:1649	Furthermore, the cell cytotoxicity test showed that the polysaccharide-based hydrogels possessed good biocompatibility.
32786859	0	36	theme	Polysaccharide-Based	0:19	arg1	Hydrogels					21:29	Polysaccharide-Based Hydrogels	0:29	Polysaccharide-Based Hydrogels Derived from Cellulose	0:52	Polysaccharide-Based Hydrogels Derived from Cellulose: The Architecture Change from Nanofibers to Hydrogels for a Putative Dual Function in Dye Wastewater Treatment.
32786859	0	37	dep	Nanofibers	84:93	arg1	to					95:96	to	95:96	to	95:96	Polysaccharide-Based Hydrogels Derived from Cellulose: The Architecture Change from Nanofibers to Hydrogels for a Putative Dual Function in Dye Wastewater Treatment.
32786859	0	37	dep	Nanofibers	84:93	arg1	Hydrogels					98:106	Hydrogels	98:106	Hydrogels	98:106	Polysaccharide-Based Hydrogels Derived from Cellulose: The Architecture Change from Nanofibers to Hydrogels for a Putative Dual Function in Dye Wastewater Treatment.
32786859	9	38	contain	has	1804:1806	arg1	strategy					1690:1697	The facile, noncytotoxic, and general strategy	1652:1697	The facile, noncytotoxic, and general strategy presented here	1652:1712	The facile, noncytotoxic, and general strategy presented here could be extended to the preparation of other polysaccharide-based hydrogel materials and has good prospects for application in wastewater treatment.
32786859	9	38	contain	has	1804:1806	arg2	prospects					1813:1821	good prospects	1808:1821	good prospects for application in wastewater treatment	1808:1861	The facile, noncytotoxic, and general strategy presented here could be extended to the preparation of other polysaccharide-based hydrogel materials and has good prospects for application in wastewater treatment.
32786859	5	39	theme	respective	957:966	arg1	advantages					968:977	respective advantages	957:977	respective advantages	957:977	To improve the purification efficiency, TiO2 nanoparticles were encapsulated into cellulose nanofibers, which were consequently changed to hydrogels with respective advantages.
32786859	6	40	theme	hydrogels	1072:1080	arg1	composition					1005:1015	chemical composition	996:1015	chemical composition	996:1015	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	6	40	theme	hydrogels	1072:1080	arg1	structure					1022:1030	structure	1022:1030	structure	1022:1030	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	6	40	theme	hydrogels	1072:1080	arg1	morphology					984:993	The morphology	980:993	The morphology	980:993	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	6	41	from	morphology	984:993	arg1	nanofibers					1118:1127	nanofibers	1118:1127	nanofibers to hydrogels	1118:1140	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	2	42	theme	food	425:428	arg1	dyes					430:433	food dyes	425:433	food dyes	425:433	One such contamination case is the environmental disposal of colored effluents from the food processing industry (i.e., food dyes).
32786859	2	42	theme	food	425:428	arg1	industry					409:416	the food processing industry	389:416	the food processing industry (i.e., food dyes)	389:434	One such contamination case is the environmental disposal of colored effluents from the food processing industry (i.e., food dyes).
32786859	5	43	theme	TiO2	843:846	arg1	nanoparticles					848:860	TiO2 nanoparticles	843:860	TiO2 nanoparticles	843:860	To improve the purification efficiency, TiO2 nanoparticles were encapsulated into cellulose nanofibers, which were consequently changed to hydrogels with respective advantages.
32786859	2	44	from	industry	409:416	arg1	case					328:331	One such contamination case	305:331	One such contamination case	305:331	One such contamination case is the environmental disposal of colored effluents from the food processing industry (i.e., food dyes).
32786859	2	44	from	industry	409:416	arg1	disposal					354:361	the environmental disposal	336:361	the environmental disposal of colored effluents from the food processing industry (i.e., food dyes)	336:434	One such contamination case is the environmental disposal of colored effluents from the food processing industry (i.e., food dyes).
32786859	2	44	from	industry	409:416	arg1	effluents					374:382	colored effluents	366:382	colored effluents from the food processing industry (i.e., food dyes)	366:434	One such contamination case is the environmental disposal of colored effluents from the food processing industry (i.e., food dyes).
32786859	6	45	theme	as-prepared	1039:1049	arg1	hydrogels					1072:1080	as-prepared polysaccharide-based hydrogels	1039:1080	as-prepared polysaccharide-based hydrogels	1039:1080	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	2	46	theme	colored	366:372	arg1	effluents					374:382	colored effluents	366:382	colored effluents from the food processing industry (i.e., food dyes)	366:434	One such contamination case is the environmental disposal of colored effluents from the food processing industry (i.e., food dyes).
32786859	6	47	theme	scanning	1159:1166	arg1	microscopy					1177:1186	scanning electron microscopy	1159:1186	scanning electron microscopy	1159:1186	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	4	48	theme	dye	779:781	arg1	wastewater					790:799	simulated dye liquor wastewater	769:799	simulated dye liquor wastewater	769:799	Here, polysaccharide-based hydrogels derived from cellulose were constructed and used in the removal of methylene blue (MB) (as the representative dye) from an aqueous medium (as simulated dye liquor wastewater).
32786859	6	49	theme	infrared	1207:1214	arg1	spectroscopy					1216:1227	infrared spectroscopy	1207:1227	infrared spectroscopy	1207:1227	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	6	50	from	nanofibers	1118:1127	arg1	hydrogels					1072:1080	as-prepared polysaccharide-based hydrogels	1039:1080	as-prepared polysaccharide-based hydrogels	1039:1080	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	6	50	from	nanofibers	1118:1127	arg1	structure					1022:1030	structure	1022:1030	structure	1022:1030	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	6	50	from	nanofibers	1118:1127	arg1	process					1105:1111	the transformation process	1086:1111	the transformation process	1086:1111	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	6	50	from	nanofibers	1118:1127	arg1	composition					1005:1015	chemical composition	996:1015	chemical composition	996:1015	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	6	50	from	nanofibers	1118:1127	arg1	morphology					984:993	The morphology	980:993	The morphology	980:993	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	2	51	theme	food	393:396	arg1	dyes					430:433	food dyes	425:433	food dyes	425:433	One such contamination case is the environmental disposal of colored effluents from the food processing industry (i.e., food dyes).
32786859	2	51	theme	food	393:396	arg1	industry					409:416	the food processing industry	389:416	the food processing industry (i.e., food dyes)	389:434	One such contamination case is the environmental disposal of colored effluents from the food processing industry (i.e., food dyes).
32786859	6	52	theme	TiO2	1329:1332	arg1	nanoparticles					1334:1346	TiO2 nanoparticles	1329:1346	TiO2 nanoparticles	1329:1346	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	1	53	theme	much	273:276	arg1	attention					278:286	much attention	273:286	much attention	273:286	Agricultural production-caused water contamination has become an urgent environmental issue that has drawn much attention in recent years.
32786859	2	54	from	disposal	354:361	arg1	dyes					430:433	food dyes	425:433	food dyes	425:433	One such contamination case is the environmental disposal of colored effluents from the food processing industry (i.e., food dyes).
32786859	2	54	from	disposal	354:361	arg1	industry					409:416	the food processing industry	389:416	the food processing industry (i.e., food dyes)	389:434	One such contamination case is the environmental disposal of colored effluents from the food processing industry (i.e., food dyes).
32786859	6	55	theme	structure	1315:1323	arg1	presence					1289:1296	the presence	1285:1296	the presence of a gel network structure and TiO2 nanoparticles	1285:1346	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	2	56	theme	such	309:312	arg1	case					328:331	One such contamination case	305:331	One such contamination case	305:331	One such contamination case is the environmental disposal of colored effluents from the food processing industry (i.e., food dyes).
32786859	2	56	theme	such	309:312	arg1	disposal					354:361	the environmental disposal	336:361	the environmental disposal of colored effluents from the food processing industry (i.e., food dyes)	336:434	One such contamination case is the environmental disposal of colored effluents from the food processing industry (i.e., food dyes).
32786859	9	57	theme	polysaccharide-based	1760:1779	arg1	materials					1790:1798	other polysaccharide-based hydrogel materials	1754:1798	other polysaccharide-based hydrogel materials	1754:1798	The facile, noncytotoxic, and general strategy presented here could be extended to the preparation of other polysaccharide-based hydrogel materials and has good prospects for application in wastewater treatment.
32786859	0	58	attach	Derived	31:37	arg1	Cellulose					44:52	Cellulose	44:52	Cellulose	44:52	Polysaccharide-Based Hydrogels Derived from Cellulose: The Architecture Change from Nanofibers to Hydrogels for a Putative Dual Function in Dye Wastewater Treatment.
32786859	0	58	attach	Derived	31:37	arg2	Hydrogels					21:29	Polysaccharide-Based Hydrogels	0:29	Polysaccharide-Based Hydrogels Derived from Cellulose	0:52	Polysaccharide-Based Hydrogels Derived from Cellulose: The Architecture Change from Nanofibers to Hydrogels for a Putative Dual Function in Dye Wastewater Treatment.
32786859	7	59	theme	photocatalytic	1504:1517	arg1	degradation					1519:1529	photocatalytic degradation	1504:1529	photocatalytic degradation	1504:1529	As expected, the polysaccharide-based hydrogels exhibited good MB removal performance because of their synergistic effects of absorption and photocatalytic degradation.
32786859	4	60	theme	polysaccharide-based	596:615	arg1	hydrogels					617:625	polysaccharide-based hydrogels	596:625	polysaccharide-based hydrogels derived from cellulose	596:648	Here, polysaccharide-based hydrogels derived from cellulose were constructed and used in the removal of methylene blue (MB) (as the representative dye) from an aqueous medium (as simulated dye liquor wastewater).
32786859	6	61	theme	gel	1303:1305	arg1	structure					1315:1323	a gel network structure	1301:1323	a gel network structure	1301:1323	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	1	62	theme	production-caused	179:195	arg1	contamination					203:215	Agricultural production-caused water contamination	166:215	Agricultural production-caused water contamination	166:215	Agricultural production-caused water contamination has become an urgent environmental issue that has drawn much attention in recent years.
32786859	7	63	theme	degradation	1519:1529	arg1	effects					1478:1484	their synergistic effects	1460:1484	their synergistic effects of absorption and photocatalytic degradation	1460:1529	As expected, the polysaccharide-based hydrogels exhibited good MB removal performance because of their synergistic effects of absorption and photocatalytic degradation.
32786859	9	64	theme	materials	1790:1798	arg1	preparation					1739:1749	the preparation	1735:1749	the preparation of other polysaccharide-based hydrogel materials	1735:1798	The facile, noncytotoxic, and general strategy presented here could be extended to the preparation of other polysaccharide-based hydrogel materials and has good prospects for application in wastewater treatment.
32786859	7	65	theme	absorption	1489:1498	arg1	effects					1478:1484	their synergistic effects	1460:1484	their synergistic effects of absorption and photocatalytic degradation	1460:1529	As expected, the polysaccharide-based hydrogels exhibited good MB removal performance because of their synergistic effects of absorption and photocatalytic degradation.
32786859	6	66	dep	Fourier	1189:1195	arg1	transform					1197:1205	transform	1197:1205	transform infrared spectroscopy	1197:1227	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	0	67	theme	Dye	140:142	arg1	Treatment					155:163	Dye Wastewater Treatment	140:163	Dye Wastewater Treatment	140:163	Polysaccharide-Based Hydrogels Derived from Cellulose: The Architecture Change from Nanofibers to Hydrogels for a Putative Dual Function in Dye Wastewater Treatment.
32786859	4	68	attach	derived	627:633	arg1	cellulose					640:648	cellulose	640:648	cellulose	640:648	Here, polysaccharide-based hydrogels derived from cellulose were constructed and used in the removal of methylene blue (MB) (as the representative dye) from an aqueous medium (as simulated dye liquor wastewater).
32786859	4	68	attach	derived	627:633	arg2	hydrogels					617:625	polysaccharide-based hydrogels	596:625	polysaccharide-based hydrogels derived from cellulose	596:648	Here, polysaccharide-based hydrogels derived from cellulose were constructed and used in the removal of methylene blue (MB) (as the representative dye) from an aqueous medium (as simulated dye liquor wastewater).
32786859	1	69	theme	urgent	231:236	arg1	issue					252:256	an urgent environmental issue	228:256	an urgent environmental issue that has drawn much attention in recent years	228:302	Agricultural production-caused water contamination has become an urgent environmental issue that has drawn much attention in recent years.
32786859	9	70	theme	wastewater	1842:1851	arg1	treatment					1853:1861	wastewater treatment	1842:1861	wastewater treatment	1842:1861	The facile, noncytotoxic, and general strategy presented here could be extended to the preparation of other polysaccharide-based hydrogel materials and has good prospects for application in wastewater treatment.
32786859	8	71	theme	cell	1549:1552	arg1	test					1567:1570	the cell cytotoxicity test	1545:1570	the cell cytotoxicity test	1545:1570	Furthermore, the cell cytotoxicity test showed that the polysaccharide-based hydrogels possessed good biocompatibility.
32786859	7	72	theme	removal	1429:1435	arg1	performance					1437:1447	good MB removal performance	1421:1447	good MB removal performance	1421:1447	As expected, the polysaccharide-based hydrogels exhibited good MB removal performance because of their synergistic effects of absorption and photocatalytic degradation.
32786859	7	73	theme	good	1421:1424	arg1	performance					1437:1447	good MB removal performance	1421:1447	good MB removal performance	1421:1447	As expected, the polysaccharide-based hydrogels exhibited good MB removal performance because of their synergistic effects of absorption and photocatalytic degradation.
32786859	4	74	theme	blue	704:707	arg1	MB					710:711	MB	710:711	MB	710:711	Here, polysaccharide-based hydrogels derived from cellulose were constructed and used in the removal of methylene blue (MB) (as the representative dye) from an aqueous medium (as simulated dye liquor wastewater).
32786859	4	74	theme	blue	704:707	arg1	methylene					694:702	methylene blue	694:707	methylene blue (MB) (as the representative dye)	694:740	Here, polysaccharide-based hydrogels derived from cellulose were constructed and used in the removal of methylene blue (MB) (as the representative dye) from an aqueous medium (as simulated dye liquor wastewater).
32786859	8	75	theme	polysaccharide-based	1588:1607	arg1	hydrogels					1609:1617	the polysaccharide-based hydrogels	1584:1617	the polysaccharide-based hydrogels	1584:1617	Furthermore, the cell cytotoxicity test showed that the polysaccharide-based hydrogels possessed good biocompatibility.
32786859	6	76	theme	chemical	996:1003	arg1	composition					1005:1015	chemical composition	996:1015	chemical composition	996:1015	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	4	77	theme	aqueous	750:756	arg1	medium					758:763	an aqueous medium	747:763	an aqueous medium (as simulated dye liquor wastewater)	747:800	Here, polysaccharide-based hydrogels derived from cellulose were constructed and used in the removal of methylene blue (MB) (as the representative dye) from an aqueous medium (as simulated dye liquor wastewater).
32786859	0	78	theme	Putative	114:121	arg1	Function					128:135	a Putative Dual Function	112:135	a Putative Dual Function in Dye Wastewater Treatment	112:163	Polysaccharide-Based Hydrogels Derived from Cellulose: The Architecture Change from Nanofibers to Hydrogels for a Putative Dual Function in Dye Wastewater Treatment.
32786859	9	79	theme	facile	1656:1661	arg1	strategy					1690:1697	The facile, noncytotoxic, and general strategy	1652:1697	The facile, noncytotoxic, and general strategy presented here	1652:1712	The facile, noncytotoxic, and general strategy presented here could be extended to the preparation of other polysaccharide-based hydrogel materials and has good prospects for application in wastewater treatment.
32786859	6	80	dep	hydrogels	1072:1080	arg1	the					1035:1037	the	1035:1037	the	1035:1037	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	9	81	theme	noncytotoxic	1664:1675	arg1	strategy					1690:1697	The facile, noncytotoxic, and general strategy	1652:1697	The facile, noncytotoxic, and general strategy presented here	1652:1712	The facile, noncytotoxic, and general strategy presented here could be extended to the preparation of other polysaccharide-based hydrogel materials and has good prospects for application in wastewater treatment.
32786859	6	82	theme	transformation	1090:1103	arg1	process					1105:1111	the transformation process	1086:1111	the transformation process	1086:1111	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	6	83	from	composition	1005:1015	arg1	nanofibers					1118:1127	nanofibers	1118:1127	nanofibers to hydrogels	1118:1140	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	2	84	theme	processing	398:407	arg1	dyes					430:433	food dyes	425:433	food dyes	425:433	One such contamination case is the environmental disposal of colored effluents from the food processing industry (i.e., food dyes).
32786859	2	84	theme	processing	398:407	arg1	industry					409:416	the food processing industry	389:416	the food processing industry (i.e., food dyes)	389:434	One such contamination case is the environmental disposal of colored effluents from the food processing industry (i.e., food dyes).
32786859	6	85	theme	polysaccharide-based	1051:1070	arg1	hydrogels					1072:1080	as-prepared polysaccharide-based hydrogels	1039:1080	as-prepared polysaccharide-based hydrogels	1039:1080	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	3	86	theme	dye	468:470	arg1	contaminants					472:483	dye contaminants	468:483	dye contaminants	468:483	Effective methods for removing dye contaminants from water have been increasingly sought, and different adsorbents have been developed for this purpose.
32786859	0	87	theme	Architecture	59:70	arg1	Change					72:77	The Architecture Change	55:77	The Architecture Change from Nanofibers to Hydrogels for a Putative Dual Function in Dye Wastewater Treatment	55:163	Polysaccharide-Based Hydrogels Derived from Cellulose: The Architecture Change from Nanofibers to Hydrogels for a Putative Dual Function in Dye Wastewater Treatment.
32786859	6	88	theme	electron	1168:1175	arg1	microscopy					1177:1186	scanning electron microscopy	1159:1186	scanning electron microscopy	1159:1186	The morphology, chemical composition, and structure of the as-prepared polysaccharide-based hydrogels and the transformation process from nanofibers to hydrogels were revealed by scanning electron microscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction, and the presence of a gel network structure and TiO2 nanoparticles was confirmed.
32786859	2	89	dep	dyes	430:433	arg1	i.e.					419:422	i.e.	419:422	i.e.	419:422	One such contamination case is the environmental disposal of colored effluents from the food processing industry (i.e., food dyes).
32786859	4	90	theme	simulated	769:777	arg1	wastewater					790:799	simulated dye liquor wastewater	769:799	simulated dye liquor wastewater	769:799	Here, polysaccharide-based hydrogels derived from cellulose were constructed and used in the removal of methylene blue (MB) (as the representative dye) from an aqueous medium (as simulated dye liquor wastewater).
32786859	2	91	theme	environmental	340:352	arg1	case					328:331	One such contamination case	305:331	One such contamination case	305:331	One such contamination case is the environmental disposal of colored effluents from the food processing industry (i.e., food dyes).
32786859	2	91	theme	environmental	340:352	arg1	disposal					354:361	the environmental disposal	336:361	the environmental disposal of colored effluents from the food processing industry (i.e., food dyes)	336:434	One such contamination case is the environmental disposal of colored effluents from the food processing industry (i.e., food dyes).
32786859	0	92	from	Function	128:135	arg1	Treatment					155:163	Dye Wastewater Treatment	140:163	Dye Wastewater Treatment	140:163	Polysaccharide-Based Hydrogels Derived from Cellulose: The Architecture Change from Nanofibers to Hydrogels for a Putative Dual Function in Dye Wastewater Treatment.
32786859	4	93	theme	liquor	783:788	arg1	wastewater					790:799	simulated dye liquor wastewater	769:799	simulated dye liquor wastewater	769:799	Here, polysaccharide-based hydrogels derived from cellulose were constructed and used in the removal of methylene blue (MB) (as the representative dye) from an aqueous medium (as simulated dye liquor wastewater).
32786859	3	94	theme	different	531:539	arg1	adsorbents					541:550	different adsorbents	531:550	different adsorbents	531:550	Effective methods for removing dye contaminants from water have been increasingly sought, and different adsorbents have been developed for this purpose.
32934292	0	0	theme	cell-macrophage	81:95	arg1	interactions					97:108	cell-macrophage interactions	81:108	cell-macrophage interactions using cyclodextrins	81:128	Quantitative analysis of red blood cell membrane phospholipids and modulation of cell-macrophage interactions using cyclodextrins.
32934292	4	1	theme	cell	685:688	arg1	integrity					699:707	cell membrane integrity	685:707	cell membrane integrity	685:707	Methyl-α-cyclodextrin, loaded with exogenous lipids, was used to extract phospholipids from the membrane outer leaflet, while delivering lipids to the cell to maintain cell membrane integrity.
32934292	8	2	used	used	1137:1140	arg2	method					1121:1126	The developed lipid exchange method	1092:1126	The developed lipid exchange method	1092:1126	The developed lipid exchange method was then used to remove phosphatidylserine, a phagocyte recognition marker, from the outer leaflet of senescent RBCs.
32934292	4	3	theme	extract	582:588	arg1	phospholipids					590:602	extract phospholipids	582:602	extract phospholipids	582:602	Methyl-α-cyclodextrin, loaded with exogenous lipids, was used to extract phospholipids from the membrane outer leaflet, while delivering lipids to the cell to maintain cell membrane integrity.
32934292	6	4	theme	RBCs	922:925	arg1	leaflet					933:939	the RBCs outer leaflet	918:939	the RBCs outer leaflet	918:939	Phosphatidylcholines (PC) and sphingomyelins (SM) were the most abundant phospholipids in the RBCs outer leaflet with PC 34:1 and SM 34:1 being the most abundant species.
32934292	4	5	used	used	574:577	arg2	Methyl-α-cyclodextrin					517:537	Methyl-α-cyclodextrin	517:537	Methyl-α-cyclodextrin	517:537	Methyl-α-cyclodextrin, loaded with exogenous lipids, was used to extract phospholipids from the membrane outer leaflet, while delivering lipids to the cell to maintain cell membrane integrity.
32934292	8	6	theme	outer	1213:1217	arg1	leaflet					1219:1225	the outer leaflet	1209:1225	the outer leaflet of senescent RBCs	1209:1243	The developed lipid exchange method was then used to remove phosphatidylserine, a phagocyte recognition marker, from the outer leaflet of senescent RBCs.
32934292	4	7	theme	membrane	690:697	arg1	integrity					699:707	cell membrane integrity	685:707	cell membrane integrity	685:707	Methyl-α-cyclodextrin, loaded with exogenous lipids, was used to extract phospholipids from the membrane outer leaflet, while delivering lipids to the cell to maintain cell membrane integrity.
32934292	6	8	theme	abundant	981:988	arg1	34:1					949:952	PC 34:1	946:952	PC 34:1	946:952	Phosphatidylcholines (PC) and sphingomyelins (SM) were the most abundant phospholipids in the RBCs outer leaflet with PC 34:1 and SM 34:1 being the most abundant species.
32934292	6	8	theme	abundant	981:988	arg1	species					990:996	the most abundant species	972:996	the most abundant species	972:996	Phosphatidylcholines (PC) and sphingomyelins (SM) were the most abundant phospholipids in the RBCs outer leaflet with PC 34:1 and SM 34:1 being the most abundant species.
32934292	6	8	theme	abundant	981:988	arg1	34:1					961:964	SM 34:1	958:964	SM 34:1	958:964	Phosphatidylcholines (PC) and sphingomyelins (SM) were the most abundant phospholipids in the RBCs outer leaflet with PC 34:1 and SM 34:1 being the most abundant species.
32934292	2	9	from	biology	331:337	arg1	disease					353:359	disease	353:359	disease	353:359	Analysis of the lipid composition of the outer leaflet is important for understanding cell membrane biology in health and disease.
32934292	2	9	from	biology	331:337	arg1	health					342:347	health	342:347	health	342:347	Analysis of the lipid composition of the outer leaflet is important for understanding cell membrane biology in health and disease.
32934292	0	10	theme	interactions	97:108	arg1	modulation					67:76	modulation	67:76	modulation of cell-macrophage interactions using cyclodextrins	67:128	Quantitative analysis of red blood cell membrane phospholipids and modulation of cell-macrophage interactions using cyclodextrins.
32934292	0	10	theme	interactions	97:108	arg1	phospholipids					49:61	red blood cell membrane phospholipids	25:61	red blood cell membrane phospholipids	25:61	Quantitative analysis of red blood cell membrane phospholipids and modulation of cell-macrophage interactions using cyclodextrins.
32934292	9	11	with	RBCs	1256:1259	arg1	membranes					1280:1288	reconstituted membranes	1266:1288	reconstituted membranes	1266:1288	Senescent RBCs with reconstituted membranes were phagocytosed in significantly lower amounts compared to control cells, demonstrating the efficiency of the lipid exchange process and its application in modifying cell-cell interactions.
32934292	6	12	from	phospholipids	901:913	arg1	leaflet					933:939	the RBCs outer leaflet	918:939	the RBCs outer leaflet	918:939	Phosphatidylcholines (PC) and sphingomyelins (SM) were the most abundant phospholipids in the RBCs outer leaflet with PC 34:1 and SM 34:1 being the most abundant species.
32934292	6	13	theme	abundant	892:899	arg1	sphingomyelins					858:871	sphingomyelins	858:871	sphingomyelins (SM)	858:876	Phosphatidylcholines (PC) and sphingomyelins (SM) were the most abundant phospholipids in the RBCs outer leaflet with PC 34:1 and SM 34:1 being the most abundant species.
32934292	6	13	theme	abundant	892:899	arg1	phospholipids					901:913	the most abundant phospholipids	883:913	the most abundant phospholipids in the RBCs outer leaflet	883:939	Phosphatidylcholines (PC) and sphingomyelins (SM) were the most abundant phospholipids in the RBCs outer leaflet with PC 34:1 and SM 34:1 being the most abundant species.
32934292	6	13	theme	abundant	892:899	arg1	Phosphatidylcholines					828:847	Phosphatidylcholines	828:847	Phosphatidylcholines (PC)	828:852	Phosphatidylcholines (PC) and sphingomyelins (SM) were the most abundant phospholipids in the RBCs outer leaflet with PC 34:1 and SM 34:1 being the most abundant species.
32934292	9	14	theme	lower	1325:1329	arg1	amounts					1331:1337	significantly lower amounts	1311:1337	significantly lower amounts compared to control cells	1311:1363	Senescent RBCs with reconstituted membranes were phagocytosed in significantly lower amounts compared to control cells, demonstrating the efficiency of the lipid exchange process and its application in modifying cell-cell interactions.
32934292	8	15	theme	senescent	1230:1238	arg1	RBCs					1240:1243	senescent RBCs	1230:1243	senescent RBCs	1230:1243	The developed lipid exchange method was then used to remove phosphatidylserine, a phagocyte recognition marker, from the outer leaflet of senescent RBCs.
32934292	5	16	theme	outer	813:817	arg1	leaflet					819:825	the membrane outer leaflet	800:825	the membrane outer leaflet	800:825	Thin layer chromatography and lipidomics demonstrated that the extracted lipids were from the membrane outer leaflet.
32934292	7	17	theme	cell	1072:1075	arg1	leaflet					1083:1089	the cell outer leaflet	1068:1089	the cell outer leaflet	1068:1089	Fluorescence quenching confirmed the delivery of exogenous lipids to the cell outer leaflet.
32934292	0	18	theme	Quantitative	0:11	arg1	analysis					13:20	Quantitative analysis	0:20	Quantitative analysis of red blood cell membrane phospholipids and modulation of cell-macrophage interactions using cyclodextrins.	0:129	Quantitative analysis of red blood cell membrane phospholipids and modulation of cell-macrophage interactions using cyclodextrins.
32934292	3	19	theme	outer	464:468	arg1	leaflet					470:476	the outer leaflet	460:476	the outer leaflet of red blood cells (RBCs)	460:502	Here, a method based on cyclodextrin-mediated lipid exchange to characterize the phospholipids in the outer leaflet of red blood cells (RBCs) is reported.
32934292	3	20	theme	cyclodextrin-mediated	386:406	arg1	exchange					414:421	cyclodextrin-mediated lipid exchange	386:421	cyclodextrin-mediated lipid exchange	386:421	Here, a method based on cyclodextrin-mediated lipid exchange to characterize the phospholipids in the outer leaflet of red blood cells (RBCs) is reported.
32934292	8	21	theme	phagocyte	1174:1182	arg1	marker					1196:1201	a phagocyte recognition marker	1172:1201	a phagocyte recognition marker	1172:1201	The developed lipid exchange method was then used to remove phosphatidylserine, a phagocyte recognition marker, from the outer leaflet of senescent RBCs.
32934292	8	21	theme	phagocyte	1174:1182	arg1	phosphatidylserine					1152:1169	phosphatidylserine	1152:1169	phosphatidylserine	1152:1169	The developed lipid exchange method was then used to remove phosphatidylserine, a phagocyte recognition marker, from the outer leaflet of senescent RBCs.
32934292	7	22	theme	outer	1077:1081	arg1	leaflet					1083:1089	the cell outer leaflet	1068:1089	the cell outer leaflet	1068:1089	Fluorescence quenching confirmed the delivery of exogenous lipids to the cell outer leaflet.
32934292	8	23	theme	developed	1096:1104	arg1	method					1121:1126	The developed lipid exchange method	1092:1126	The developed lipid exchange method	1092:1126	The developed lipid exchange method was then used to remove phosphatidylserine, a phagocyte recognition marker, from the outer leaflet of senescent RBCs.
32934292	3	24	theme	lipid	408:412	arg1	exchange					414:421	cyclodextrin-mediated lipid exchange	386:421	cyclodextrin-mediated lipid exchange	386:421	Here, a method based on cyclodextrin-mediated lipid exchange to characterize the phospholipids in the outer leaflet of red blood cells (RBCs) is reported.
32934292	8	25	theme	RBCs	1240:1243	arg1	leaflet					1219:1225	the outer leaflet	1209:1225	the outer leaflet of senescent RBCs	1209:1243	The developed lipid exchange method was then used to remove phosphatidylserine, a phagocyte recognition marker, from the outer leaflet of senescent RBCs.
32934292	0	26	theme	red	25:27	arg1	phospholipids					49:61	red blood cell membrane phospholipids	25:61	red blood cell membrane phospholipids	25:61	Quantitative analysis of red blood cell membrane phospholipids and modulation of cell-macrophage interactions using cyclodextrins.
32934292	9	27	theme	control	1351:1357	arg1	cells					1359:1363	control cells	1351:1363	control cells	1351:1363	Senescent RBCs with reconstituted membranes were phagocytosed in significantly lower amounts compared to control cells, demonstrating the efficiency of the lipid exchange process and its application in modifying cell-cell interactions.
32934292	2	28	theme	membrane	322:329	arg1	biology					331:337	cell membrane biology	317:337	cell membrane biology in health and disease	317:359	Analysis of the lipid composition of the outer leaflet is important for understanding cell membrane biology in health and disease.
32934292	1	29	theme	phospholipid	205:216	arg1	composition					218:228	its phospholipid composition	201:228	its phospholipid composition	201:228	The plasma membrane of eukaryotic cells is asymmetric with respect to its phospholipid composition.
32934292	6	30	theme	SM	958:959	arg1	34:1					949:952	PC 34:1	946:952	PC 34:1	946:952	Phosphatidylcholines (PC) and sphingomyelins (SM) were the most abundant phospholipids in the RBCs outer leaflet with PC 34:1 and SM 34:1 being the most abundant species.
32934292	6	30	theme	SM	958:959	arg1	species					990:996	the most abundant species	972:996	the most abundant species	972:996	Phosphatidylcholines (PC) and sphingomyelins (SM) were the most abundant phospholipids in the RBCs outer leaflet with PC 34:1 and SM 34:1 being the most abundant species.
32934292	6	30	theme	SM	958:959	arg1	34:1					961:964	SM 34:1	958:964	SM 34:1	958:964	Phosphatidylcholines (PC) and sphingomyelins (SM) were the most abundant phospholipids in the RBCs outer leaflet with PC 34:1 and SM 34:1 being the most abundant species.
32934292	5	31	theme	Thin	710:713	arg1	chromatography					721:734	Thin layer chromatography	710:734	Thin layer chromatography	710:734	Thin layer chromatography and lipidomics demonstrated that the extracted lipids were from the membrane outer leaflet.
32934292	2	32	theme	cell	317:320	arg1	biology					331:337	cell membrane biology	317:337	cell membrane biology in health and disease	317:359	Analysis of the lipid composition of the outer leaflet is important for understanding cell membrane biology in health and disease.
32934292	3	33	theme	blood	485:489	arg1	RBCs					498:501	RBCs	498:501	RBCs	498:501	Here, a method based on cyclodextrin-mediated lipid exchange to characterize the phospholipids in the outer leaflet of red blood cells (RBCs) is reported.
32934292	3	33	theme	blood	485:489	arg1	cells					491:495	red blood cells	481:495	red blood cells (RBCs)	481:502	Here, a method based on cyclodextrin-mediated lipid exchange to characterize the phospholipids in the outer leaflet of red blood cells (RBCs) is reported.
32934292	0	34	theme	cell	35:38	arg1	phospholipids					49:61	red blood cell membrane phospholipids	25:61	red blood cell membrane phospholipids	25:61	Quantitative analysis of red blood cell membrane phospholipids and modulation of cell-macrophage interactions using cyclodextrins.
32934292	5	35	theme	layer	715:719	arg1	chromatography					721:734	Thin layer chromatography	710:734	Thin layer chromatography	710:734	Thin layer chromatography and lipidomics demonstrated that the extracted lipids were from the membrane outer leaflet.
32934292	2	36	theme	composition	253:263	arg1	Analysis					231:238	Analysis	231:238	Analysis of the lipid composition of the outer leaflet	231:284	Analysis of the lipid composition of the outer leaflet is important for understanding cell membrane biology in health and disease.
32934292	0	37	theme	blood	29:33	arg1	phospholipids					49:61	red blood cell membrane phospholipids	25:61	red blood cell membrane phospholipids	25:61	Quantitative analysis of red blood cell membrane phospholipids and modulation of cell-macrophage interactions using cyclodextrins.
32934292	2	38	theme	lipid	247:251	arg1	composition					253:263	the lipid composition	243:263	the lipid composition of the outer leaflet	243:284	Analysis of the lipid composition of the outer leaflet is important for understanding cell membrane biology in health and disease.
32934292	8	39	theme	recognition	1184:1194	arg1	marker					1196:1201	a phagocyte recognition marker	1172:1201	a phagocyte recognition marker	1172:1201	The developed lipid exchange method was then used to remove phosphatidylserine, a phagocyte recognition marker, from the outer leaflet of senescent RBCs.
32934292	8	39	theme	recognition	1184:1194	arg1	phosphatidylserine					1152:1169	phosphatidylserine	1152:1169	phosphatidylserine	1152:1169	The developed lipid exchange method was then used to remove phosphatidylserine, a phagocyte recognition marker, from the outer leaflet of senescent RBCs.
32934292	0	40	theme	phospholipids	49:61	arg1	analysis					13:20	Quantitative analysis	0:20	Quantitative analysis of red blood cell membrane phospholipids and modulation of cell-macrophage interactions using cyclodextrins.	0:129	Quantitative analysis of red blood cell membrane phospholipids and modulation of cell-macrophage interactions using cyclodextrins.
32934292	6	41	theme	PC	946:947	arg1	34:1					961:964	SM 34:1	958:964	SM 34:1	958:964	Phosphatidylcholines (PC) and sphingomyelins (SM) were the most abundant phospholipids in the RBCs outer leaflet with PC 34:1 and SM 34:1 being the most abundant species.
32934292	6	41	theme	PC	946:947	arg1	34:1					949:952	PC 34:1	946:952	PC 34:1	946:952	Phosphatidylcholines (PC) and sphingomyelins (SM) were the most abundant phospholipids in the RBCs outer leaflet with PC 34:1 and SM 34:1 being the most abundant species.
32934292	6	41	theme	PC	946:947	arg1	species					990:996	the most abundant species	972:996	the most abundant species	972:996	Phosphatidylcholines (PC) and sphingomyelins (SM) were the most abundant phospholipids in the RBCs outer leaflet with PC 34:1 and SM 34:1 being the most abundant species.
32934292	8	42	theme	lipid	1106:1110	arg1	method					1121:1126	The developed lipid exchange method	1092:1126	The developed lipid exchange method	1092:1126	The developed lipid exchange method was then used to remove phosphatidylserine, a phagocyte recognition marker, from the outer leaflet of senescent RBCs.
32934292	5	43	theme	extracted	773:781	arg1	lipids					783:788	the extracted lipids	769:788	the extracted lipids	769:788	Thin layer chromatography and lipidomics demonstrated that the extracted lipids were from the membrane outer leaflet.
32934292	0	44	theme	membrane	40:47	arg1	phospholipids					49:61	red blood cell membrane phospholipids	25:61	red blood cell membrane phospholipids	25:61	Quantitative analysis of red blood cell membrane phospholipids and modulation of cell-macrophage interactions using cyclodextrins.
32934292	4	45	theme	exogenous	552:560	arg1	lipids					562:567	exogenous lipids	552:567	exogenous lipids	552:567	Methyl-α-cyclodextrin, loaded with exogenous lipids, was used to extract phospholipids from the membrane outer leaflet, while delivering lipids to the cell to maintain cell membrane integrity.
32934292	7	46	theme	exogenous	1048:1056	arg1	lipids					1058:1063	exogenous lipids	1048:1063	exogenous lipids	1048:1063	Fluorescence quenching confirmed the delivery of exogenous lipids to the cell outer leaflet.
32934292	9	47	theme	Senescent	1246:1254	arg1	RBCs					1256:1259	Senescent RBCs	1246:1259	Senescent RBCs with reconstituted membranes	1246:1288	Senescent RBCs with reconstituted membranes were phagocytosed in significantly lower amounts compared to control cells, demonstrating the efficiency of the lipid exchange process and its application in modifying cell-cell interactions.
32934292	1	48	theme	plasma	135:140	arg1	membrane					142:149	The plasma membrane	131:149	The plasma membrane of eukaryotic cells	131:169	The plasma membrane of eukaryotic cells is asymmetric with respect to its phospholipid composition.
32934292	1	48	theme	plasma	135:140	arg1	asymmetric					174:183	asymmetric	174:183	asymmetric	174:183	The plasma membrane of eukaryotic cells is asymmetric with respect to its phospholipid composition.
32934292	2	49	theme	leaflet	278:284	arg1	composition					253:263	the lipid composition	243:263	the lipid composition of the outer leaflet	243:284	Analysis of the lipid composition of the outer leaflet is important for understanding cell membrane biology in health and disease.
32934292	7	50	theme	Fluorescence	999:1010	arg1	quenching					1012:1020	Fluorescence quenching	999:1020	Fluorescence quenching	999:1020	Fluorescence quenching confirmed the delivery of exogenous lipids to the cell outer leaflet.
32934292	3	51	theme	cells	491:495	arg1	leaflet					470:476	the outer leaflet	460:476	the outer leaflet of red blood cells (RBCs)	460:502	Here, a method based on cyclodextrin-mediated lipid exchange to characterize the phospholipids in the outer leaflet of red blood cells (RBCs) is reported.
32934292	4	52	theme	membrane	613:620	arg1	leaflet					628:634	the membrane outer leaflet	609:634	the membrane outer leaflet	609:634	Methyl-α-cyclodextrin, loaded with exogenous lipids, was used to extract phospholipids from the membrane outer leaflet, while delivering lipids to the cell to maintain cell membrane integrity.
32934292	8	53	theme	exchange	1112:1119	arg1	method					1121:1126	The developed lipid exchange method	1092:1126	The developed lipid exchange method	1092:1126	The developed lipid exchange method was then used to remove phosphatidylserine, a phagocyte recognition marker, from the outer leaflet of senescent RBCs.
32934292	2	54	theme	outer	272:276	arg1	leaflet					278:284	the outer leaflet	268:284	the outer leaflet	268:284	Analysis of the lipid composition of the outer leaflet is important for understanding cell membrane biology in health and disease.
32934292	3	55	from	phospholipids	443:455	arg1	leaflet					470:476	the outer leaflet	460:476	the outer leaflet of red blood cells (RBCs)	460:502	Here, a method based on cyclodextrin-mediated lipid exchange to characterize the phospholipids in the outer leaflet of red blood cells (RBCs) is reported.
32934292	9	56	theme	lipid	1402:1406	arg1	process					1417:1423	the lipid exchange process	1398:1423	the lipid exchange process	1398:1423	Senescent RBCs with reconstituted membranes were phagocytosed in significantly lower amounts compared to control cells, demonstrating the efficiency of the lipid exchange process and its application in modifying cell-cell interactions.
32934292	0	57	theme	modulation	67:76	arg1	analysis					13:20	Quantitative analysis	0:20	Quantitative analysis of red blood cell membrane phospholipids and modulation of cell-macrophage interactions using cyclodextrins.	0:129	Quantitative analysis of red blood cell membrane phospholipids and modulation of cell-macrophage interactions using cyclodextrins.
32934292	9	58	theme	reconstituted	1266:1278	arg1	membranes					1280:1288	reconstituted membranes	1266:1288	reconstituted membranes	1266:1288	Senescent RBCs with reconstituted membranes were phagocytosed in significantly lower amounts compared to control cells, demonstrating the efficiency of the lipid exchange process and its application in modifying cell-cell interactions.
32934292	3	59	theme	red	481:483	arg1	RBCs					498:501	RBCs	498:501	RBCs	498:501	Here, a method based on cyclodextrin-mediated lipid exchange to characterize the phospholipids in the outer leaflet of red blood cells (RBCs) is reported.
32934292	3	59	theme	red	481:483	arg1	cells					491:495	red blood cells	481:495	red blood cells (RBCs)	481:502	Here, a method based on cyclodextrin-mediated lipid exchange to characterize the phospholipids in the outer leaflet of red blood cells (RBCs) is reported.
32934292	1	60	theme	eukaryotic	154:163	arg1	cells					165:169	eukaryotic cells	154:169	eukaryotic cells	154:169	The plasma membrane of eukaryotic cells is asymmetric with respect to its phospholipid composition.
32934292	9	61	theme	cell-cell	1458:1466	arg1	interactions					1468:1479	cell-cell interactions	1458:1479	cell-cell interactions	1458:1479	Senescent RBCs with reconstituted membranes were phagocytosed in significantly lower amounts compared to control cells, demonstrating the efficiency of the lipid exchange process and its application in modifying cell-cell interactions.
32934292	9	62	theme	exchange	1408:1415	arg1	process					1417:1423	the lipid exchange process	1398:1423	the lipid exchange process	1398:1423	Senescent RBCs with reconstituted membranes were phagocytosed in significantly lower amounts compared to control cells, demonstrating the efficiency of the lipid exchange process and its application in modifying cell-cell interactions.
32934292	5	63	theme	membrane	804:811	arg1	leaflet					819:825	the membrane outer leaflet	800:825	the membrane outer leaflet	800:825	Thin layer chromatography and lipidomics demonstrated that the extracted lipids were from the membrane outer leaflet.
32934292	6	64	theme	outer	927:931	arg1	leaflet					933:939	the RBCs outer leaflet	918:939	the RBCs outer leaflet	918:939	Phosphatidylcholines (PC) and sphingomyelins (SM) were the most abundant phospholipids in the RBCs outer leaflet with PC 34:1 and SM 34:1 being the most abundant species.
32934292	7	65	theme	lipids	1058:1063	arg1	delivery					1036:1043	the delivery	1032:1043	the delivery of exogenous lipids to the cell outer leaflet	1032:1089	Fluorescence quenching confirmed the delivery of exogenous lipids to the cell outer leaflet.
32934292	1	66	theme	cells	165:169	arg1	membrane					142:149	The plasma membrane	131:149	The plasma membrane of eukaryotic cells	131:169	The plasma membrane of eukaryotic cells is asymmetric with respect to its phospholipid composition.
32934292	1	66	theme	cells	165:169	arg1	asymmetric					174:183	asymmetric	174:183	asymmetric	174:183	The plasma membrane of eukaryotic cells is asymmetric with respect to its phospholipid composition.
32934292	9	67	theme	process	1417:1423	arg1	application					1433:1443	its application	1429:1443	its application in modifying cell-cell interactions	1429:1479	Senescent RBCs with reconstituted membranes were phagocytosed in significantly lower amounts compared to control cells, demonstrating the efficiency of the lipid exchange process and its application in modifying cell-cell interactions.
32934292	9	67	theme	process	1417:1423	arg1	efficiency					1384:1393	the efficiency	1380:1393	the efficiency of the lipid exchange process	1380:1423	Senescent RBCs with reconstituted membranes were phagocytosed in significantly lower amounts compared to control cells, demonstrating the efficiency of the lipid exchange process and its application in modifying cell-cell interactions.
32934292	4	68	theme	outer	622:626	arg1	leaflet					628:634	the membrane outer leaflet	609:634	the membrane outer leaflet	609:634	Methyl-α-cyclodextrin, loaded with exogenous lipids, was used to extract phospholipids from the membrane outer leaflet, while delivering lipids to the cell to maintain cell membrane integrity.
33338531	0	0	theme	maltose	85:91	arg1	production					66:75	the production	62:75	the production of high maltose containing syrup	62:108	Immobilization of α-amylase on GO-magnetite nanoparticles for the production of high maltose containing syrup.
33338531	9	1	from	13 h	1113:1116	arg1	buffer					1181:1186	20 mM phosphate buffer	1165:1186	20 mM phosphate buffer	1165:1186	The half-life of soluble amylase was 13 h, which enhanced to 20 h upon immobilization in 20 mM phosphate buffer, pH 7 at 50 °C. Besides, the thermodynamic parameters supported the stability trends.
33338531	7	2	theme	77.58 μg	826:833	arg1	amylase					835:841	77.58 μg amylase	826:841	77.58 μg amylase	826:841	High loading capacity of 77.58 μg amylase over 1 μg GO-magnetite nanoparticles was achieved under optimum conditions.
33338531	0	3	theme	high	80:83	arg1	maltose					85:91	high maltose	80:91	high maltose containing syrup	80:108	Immobilization of α-amylase on GO-magnetite nanoparticles for the production of high maltose containing syrup.
33338531	1	4	from	multitude	159:167	arg1	amylases					118:125	Robust amylases	111:125	Robust amylases with stability and catalysis at multitude of extremities	111:182	Robust amylases with stability and catalysis at multitude of extremities are the need of an hour.
33338531	1	4	from	multitude	159:167	arg1	need					192:195	the need	188:195	the need of an hour	188:206	Robust amylases with stability and catalysis at multitude of extremities are the need of an hour.
33338531	7	5	theme	loading	806:812	arg1	capacity					814:821	High loading capacity	801:821	High loading capacity of 77.58 μg amylase over 1 μg GO-magnetite nanoparticles	801:878	High loading capacity of 77.58 μg amylase over 1 μg GO-magnetite nanoparticles was achieved under optimum conditions.
33338531	11	6	theme	mentioned	1342:1350	arg1	attributes					1352:1361	The mentioned attributes	1338:1361	The mentioned attributes	1338:1361	The mentioned attributes and the dextrose equivalent values during the production of high maltose containing syrup highlighted its commercialization.
33338531	0	7	from	Immobilization	0:13	arg1	GO-magnetite					31:42	GO-magnetite	31:42	GO-magnetite	31:42	Immobilization of α-amylase on GO-magnetite nanoparticles for the production of high maltose containing syrup.
33338531	3	8	dep	oxide	383:387	arg1	GO					390:391	GO	390:391	GO	390:391	In the present study, a commercially available amylase was immobilized on graphene oxide (GO) - magnetite (Fe3O4) nanoparticles through covalent bonding.
33338531	10	9	theme	subsequent	1319:1328	arg1	cycles					1330:1335	11 subsequent cycles	1316:1335	11 subsequent cycles	1316:1335	The immobilized amylase could be used for 11 subsequent cycles.
33338531	4	10	theme	structural	458:467	arg1	characterizations					487:503	The structural and morphological characterizations	454:503	The structural and morphological characterizations	454:503	The structural and morphological characterizations were conducted by XRD, SEM and TEM.
33338531	7	11	theme	optimum	899:905	arg1	conditions					907:916	optimum conditions	899:916	optimum conditions	899:916	High loading capacity of 77.58 μg amylase over 1 μg GO-magnetite nanoparticles was achieved under optimum conditions.
33338531	2	12	theme	such	283:286	arg1	attributes					288:297	such attributes	283:297	such attributes	283:297	Enzyme immobilization may prove beneficial at commercial scale to achieve such attributes.
33338531	11	13	theme	dextrose	1371:1378	arg1	values					1391:1396	the dextrose equivalent values	1367:1396	the dextrose equivalent values during the production of high maltose containing syrup	1367:1451	The mentioned attributes and the dextrose equivalent values during the production of high maltose containing syrup highlighted its commercialization.
33338531	3	14	theme	available	337:345	arg1	amylase					347:353	a commercially available amylase	322:353	a commercially available amylase	322:353	In the present study, a commercially available amylase was immobilized on graphene oxide (GO) - magnetite (Fe3O4) nanoparticles through covalent bonding.
33338531	8	15	theme	pH	938:939	arg1	optimum					941:947	the pH optimum	934:947	the pH optimum	934:947	Biochemically, the pH optimum remained unaltered, i.e., pH 7, whereas, the alkalitolerance was increased by ~20% in relative activities upon immobilization.
33338531	7	16	theme	High	801:804	arg1	capacity					814:821	High loading capacity	801:821	High loading capacity of 77.58 μg amylase over 1 μg GO-magnetite nanoparticles	801:878	High loading capacity of 77.58 μg amylase over 1 μg GO-magnetite nanoparticles was achieved under optimum conditions.
33338531	1	17	theme	extremities	172:182	arg1	multitude					159:167	multitude	159:167	multitude of extremities	159:182	Robust amylases with stability and catalysis at multitude of extremities are the need of an hour.
33338531	4	18	theme	morphological	473:485	arg1	characterizations					487:503	The structural and morphological characterizations	454:503	The structural and morphological characterizations	454:503	The structural and morphological characterizations were conducted by XRD, SEM and TEM.
33338531	8	19	dep	pH 7	975:978	arg1	i.e.					969:972	i.e.	969:972	i.e.	969:972	Biochemically, the pH optimum remained unaltered, i.e., pH 7, whereas, the alkalitolerance was increased by ~20% in relative activities upon immobilization.
33338531	3	20	theme	oxide	383:387	arg1	nanoparticles					414:426	graphene oxide (GO) - magnetite (Fe3O4) nanoparticles	374:426	graphene oxide (GO) - magnetite (Fe3O4) nanoparticles	374:426	In the present study, a commercially available amylase was immobilized on graphene oxide (GO) - magnetite (Fe3O4) nanoparticles through covalent bonding.
33338531	9	21	theme	stability	1256:1264	arg1	trends					1266:1271	the stability trends	1252:1271	the stability trends	1252:1271	The half-life of soluble amylase was 13 h, which enhanced to 20 h upon immobilization in 20 mM phosphate buffer, pH 7 at 50 °C. Besides, the thermodynamic parameters supported the stability trends.
33338531	1	22	with	amylases	118:125	arg1	stability					132:140	stability	132:140	stability	132:140	Robust amylases with stability and catalysis at multitude of extremities are the need of an hour.
33338531	1	22	with	amylases	118:125	arg1	catalysis					146:154	catalysis	146:154	catalysis	146:154	Robust amylases with stability and catalysis at multitude of extremities are the need of an hour.
33338531	6	23	theme	GO	669:670	arg1	concentrations					651:664	concentrations	651:664	concentrations of GO (1.3 mg), Fe3O4 (58 μg), and amylase (4.5 mg)	651:716	The variables, such as concentrations of GO (1.3 mg), Fe3O4 (58 μg), and amylase (4.5 mg) were optimized by the response surface methodology using central composite design.
33338531	10	24	theme	immobilized	1278:1288	arg1	amylase					1290:1296	The immobilized amylase	1274:1296	The immobilized amylase	1274:1296	The immobilized amylase could be used for 11 subsequent cycles.
33338531	6	25	theme	response	740:747	arg1	methodology					757:767	the response surface methodology	736:767	the response surface methodology using central composite design	736:798	The variables, such as concentrations of GO (1.3 mg), Fe3O4 (58 μg), and amylase (4.5 mg) were optimized by the response surface methodology using central composite design.
33338531	3	26	theme	graphene	374:381	arg1	oxide					383:387	graphene oxide	374:387	graphene oxide (GO)	374:392	In the present study, a commercially available amylase was immobilized on graphene oxide (GO) - magnetite (Fe3O4) nanoparticles through covalent bonding.
33338531	11	27	theme	containing	1436:1445	arg1	syrup					1447:1451	high maltose containing syrup	1423:1451	high maltose containing syrup	1423:1451	The mentioned attributes and the dextrose equivalent values during the production of high maltose containing syrup highlighted its commercialization.
33338531	1	28	theme	Robust	111:116	arg1	amylases					118:125	Robust amylases	111:125	Robust amylases with stability and catalysis at multitude of extremities	111:182	Robust amylases with stability and catalysis at multitude of extremities are the need of an hour.
33338531	1	28	theme	Robust	111:116	arg1	need					192:195	the need	188:195	the need of an hour	188:206	Robust amylases with stability and catalysis at multitude of extremities are the need of an hour.
33338531	11	29	theme	equivalent	1380:1389	arg1	values					1391:1396	the dextrose equivalent values	1367:1396	the dextrose equivalent values during the production of high maltose containing syrup	1367:1451	The mentioned attributes and the dextrose equivalent values during the production of high maltose containing syrup highlighted its commercialization.
33338531	9	30	theme	20 mM	1165:1169	arg1	buffer					1181:1186	20 mM phosphate buffer	1165:1186	20 mM phosphate buffer	1165:1186	The half-life of soluble amylase was 13 h, which enhanced to 20 h upon immobilization in 20 mM phosphate buffer, pH 7 at 50 °C. Besides, the thermodynamic parameters supported the stability trends.
33338531	11	31	theme	high	1423:1426	arg1	syrup					1447:1451	high maltose containing syrup	1423:1451	high maltose containing syrup	1423:1451	The mentioned attributes and the dextrose equivalent values during the production of high maltose containing syrup highlighted its commercialization.
33338531	1	32	theme	hour	203:206	arg1	amylases					118:125	Robust amylases	111:125	Robust amylases with stability and catalysis at multitude of extremities	111:182	Robust amylases with stability and catalysis at multitude of extremities are the need of an hour.
33338531	1	32	theme	hour	203:206	arg1	need					192:195	the need	188:195	the need of an hour	188:206	Robust amylases with stability and catalysis at multitude of extremities are the need of an hour.
33338531	3	33	theme	magnetite	396:404	arg1	nanoparticles					414:426	graphene oxide (GO) - magnetite (Fe3O4) nanoparticles	374:426	graphene oxide (GO) - magnetite (Fe3O4) nanoparticles	374:426	In the present study, a commercially available amylase was immobilized on graphene oxide (GO) - magnetite (Fe3O4) nanoparticles through covalent bonding.
33338531	9	34	theme	phosphate	1171:1179	arg1	buffer					1181:1186	20 mM phosphate buffer	1165:1186	20 mM phosphate buffer	1165:1186	The half-life of soluble amylase was 13 h, which enhanced to 20 h upon immobilization in 20 mM phosphate buffer, pH 7 at 50 °C. Besides, the thermodynamic parameters supported the stability trends.
33338531	6	35	theme	amylase	701:707	arg1	concentrations					651:664	concentrations	651:664	concentrations of GO (1.3 mg), Fe3O4 (58 μg), and amylase (4.5 mg)	651:716	The variables, such as concentrations of GO (1.3 mg), Fe3O4 (58 μg), and amylase (4.5 mg) were optimized by the response surface methodology using central composite design.
33338531	3	36	theme	covalent	436:443	arg1	bonding					445:451	covalent bonding	436:451	covalent bonding	436:451	In the present study, a commercially available amylase was immobilized on graphene oxide (GO) - magnetite (Fe3O4) nanoparticles through covalent bonding.
33338531	8	37	theme	relative	1035:1042	arg1	activities					1044:1053	relative activities	1035:1053	relative activities upon immobilization	1035:1073	Biochemically, the pH optimum remained unaltered, i.e., pH 7, whereas, the alkalitolerance was increased by ~20% in relative activities upon immobilization.
33338531	6	38	theme	composite	783:791	arg1	design					793:798	central composite design	775:798	central composite design	775:798	The variables, such as concentrations of GO (1.3 mg), Fe3O4 (58 μg), and amylase (4.5 mg) were optimized by the response surface methodology using central composite design.
33338531	11	39	theme	syrup	1447:1451	arg1	production					1409:1418	the production	1405:1418	the production of high maltose containing syrup	1405:1451	The mentioned attributes and the dextrose equivalent values during the production of high maltose containing syrup highlighted its commercialization.
33338531	9	40	theme	thermodynamic	1217:1229	arg1	parameters					1231:1240	the thermodynamic parameters	1213:1240	the thermodynamic parameters	1213:1240	The half-life of soluble amylase was 13 h, which enhanced to 20 h upon immobilization in 20 mM phosphate buffer, pH 7 at 50 °C. Besides, the thermodynamic parameters supported the stability trends.
33338531	3	41	theme	present	307:313	arg1	study					315:319	the present study	303:319	the present study	303:319	In the present study, a commercially available amylase was immobilized on graphene oxide (GO) - magnetite (Fe3O4) nanoparticles through covalent bonding.
33338531	6	42	theme	central	775:781	arg1	design					793:798	central composite design	775:798	central composite design	775:798	The variables, such as concentrations of GO (1.3 mg), Fe3O4 (58 μg), and amylase (4.5 mg) were optimized by the response surface methodology using central composite design.
33338531	2	43	theme	Enzyme	209:214	arg1	immobilization					216:229	Enzyme immobilization	209:229	Enzyme immobilization	209:229	Enzyme immobilization may prove beneficial at commercial scale to achieve such attributes.
33338531	0	44	contain	containing	93:102	arg2	syrup					104:108	syrup	104:108	syrup	104:108	Immobilization of α-amylase on GO-magnetite nanoparticles for the production of high maltose containing syrup.
33338531	0	44	contain	containing	93:102	arg1	maltose					85:91	high maltose	80:91	high maltose containing syrup	80:108	Immobilization of α-amylase on GO-magnetite nanoparticles for the production of high maltose containing syrup.
33338531	7	45	theme	1 μg	848:851	arg1	nanoparticles					866:878	1 μg GO-magnetite nanoparticles	848:878	1 μg GO-magnetite nanoparticles	848:878	High loading capacity of 77.58 μg amylase over 1 μg GO-magnetite nanoparticles was achieved under optimum conditions.
33338531	9	46	theme	amylase	1101:1107	arg1	13 h					1113:1116	13 h	1113:1116	13 h	1113:1116	The half-life of soluble amylase was 13 h, which enhanced to 20 h upon immobilization in 20 mM phosphate buffer, pH 7 at 50 °C. Besides, the thermodynamic parameters supported the stability trends.
33338531	9	46	theme	amylase	1101:1107	arg1	half-life					1080:1088	The half-life	1076:1088	The half-life of soluble amylase	1076:1107	The half-life of soluble amylase was 13 h, which enhanced to 20 h upon immobilization in 20 mM phosphate buffer, pH 7 at 50 °C. Besides, the thermodynamic parameters supported the stability trends.
33338531	7	47	theme	GO-magnetite	853:864	arg1	nanoparticles					866:878	1 μg GO-magnetite nanoparticles	848:878	1 μg GO-magnetite nanoparticles	848:878	High loading capacity of 77.58 μg amylase over 1 μg GO-magnetite nanoparticles was achieved under optimum conditions.
33338531	2	48	theme	commercial	255:264	arg1	scale					266:270	commercial scale	255:270	commercial scale to achieve such attributes	255:297	Enzyme immobilization may prove beneficial at commercial scale to achieve such attributes.
33338531	7	49	theme	amylase	835:841	arg1	capacity					814:821	High loading capacity	801:821	High loading capacity of 77.58 μg amylase over 1 μg GO-magnetite nanoparticles	801:878	High loading capacity of 77.58 μg amylase over 1 μg GO-magnetite nanoparticles was achieved under optimum conditions.
33338531	6	50	theme	surface	749:755	arg1	methodology					757:767	the response surface methodology	736:767	the response surface methodology using central composite design	736:798	The variables, such as concentrations of GO (1.3 mg), Fe3O4 (58 μg), and amylase (4.5 mg) were optimized by the response surface methodology using central composite design.
33338531	9	51	theme	soluble	1093:1099	arg1	amylase					1101:1107	soluble amylase	1093:1107	soluble amylase	1093:1107	The half-life of soluble amylase was 13 h, which enhanced to 20 h upon immobilization in 20 mM phosphate buffer, pH 7 at 50 °C. Besides, the thermodynamic parameters supported the stability trends.
33338531	10	52	used	used	1307:1310	arg2	amylase					1290:1296	The immobilized amylase	1274:1296	The immobilized amylase	1274:1296	The immobilized amylase could be used for 11 subsequent cycles.
33338531	9	53	from	50 °C.	1197:1202	arg1	13 h					1113:1116	13 h	1113:1116	13 h	1113:1116	The half-life of soluble amylase was 13 h, which enhanced to 20 h upon immobilization in 20 mM phosphate buffer, pH 7 at 50 °C. Besides, the thermodynamic parameters supported the stability trends.
33338531	0	54	theme	α-amylase	18:26	arg1	Immobilization					0:13	Immobilization	0:13	Immobilization of α-amylase on GO-magnetite	0:42	Immobilization of α-amylase on GO-magnetite nanoparticles for the production of high maltose containing syrup.
33338531	6	55	theme	Fe3O4	682:686	arg1	concentrations					651:664	concentrations	651:664	concentrations of GO (1.3 mg), Fe3O4 (58 μg), and amylase (4.5 mg)	651:716	The variables, such as concentrations of GO (1.3 mg), Fe3O4 (58 μg), and amylase (4.5 mg) were optimized by the response surface methodology using central composite design.
33338531	11	56	theme	maltose	1428:1434	arg1	syrup					1447:1451	high maltose containing syrup	1423:1451	high maltose containing syrup	1423:1451	The mentioned attributes and the dextrose equivalent values during the production of high maltose containing syrup highlighted its commercialization.
32409034	2	0	theme	agent	390:394	arg1	use					358:360	the use	354:360	the use of any solvent and reducing agent	354:394	First silica supported silver nanoparticles (CAB-O-SIL-Ag) were prepared via solid state sintering route without the use of any solvent and reducing agent.
32409034	1	1	contain	containing	138:147	arg1	films					132:136	Alginate films	123:136	Alginate films containing pyrogenic silica supported silver nanoparticles	123:195	Alginate films containing pyrogenic silica supported silver nanoparticles were prepared as potential wound dressings.
32409034	1	1	contain	containing	138:147	arg2	nanoparticles					183:195	pyrogenic silica supported silver nanoparticles	149:195	pyrogenic silica supported silver nanoparticles	149:195	Alginate films containing pyrogenic silica supported silver nanoparticles were prepared as potential wound dressings.
32409034	1	1	contain	containing	138:147	arg1	dressings					230:238	potential wound dressings	214:238	potential wound dressings	214:238	Alginate films containing pyrogenic silica supported silver nanoparticles were prepared as potential wound dressings.
32409034	2	2	theme	reducing	381:388	arg1	agent					390:394	any solvent and reducing agent	365:394	agent	390:394	First silica supported silver nanoparticles (CAB-O-SIL-Ag) were prepared via solid state sintering route without the use of any solvent and reducing agent.
32409034	3	3	dep	nanoparticles	581:593	arg1	grown					621:625	grown	621:625	grown on the silica surface	621:647	The obtained composite was characterized by X-ray powder diffraction, transmission electron microscopy and UV-vis spectroscopy which evidenced the presence of 8-20 nm spherical silver nanoparticles uniformly distributed and grown on the silica surface.
32409034	3	3	dep	nanoparticles	581:593	arg1	distributed					605:615	distributed	605:615	distributed	605:615	The obtained composite was characterized by X-ray powder diffraction, transmission electron microscopy and UV-vis spectroscopy which evidenced the presence of 8-20 nm spherical silver nanoparticles uniformly distributed and grown on the silica surface.
32409034	0	4	theme	antibiofilm	101:111	arg1	activity					113:120	antibiofilm activity	101:120	antibiofilm activity	101:120	Biocompatible alginate silica supported silver nanoparticles composite films for wound dressing with antibiofilm activity.
32409034	6	5	theme	silver	876:881	arg1	release					883:889	a very slow silver release	864:889	a very slow silver release	864:889	They showed good hydration properties and a very slow silver release.
32409034	3	6	theme	8-20 nm	556:562	arg1	nanoparticles					581:593	8-20 nm spherical silver nanoparticles	556:593	8-20 nm spherical silver nanoparticles uniformly distributed and grown on the silica surface	556:647	The obtained composite was characterized by X-ray powder diffraction, transmission electron microscopy and UV-vis spectroscopy which evidenced the presence of 8-20 nm spherical silver nanoparticles uniformly distributed and grown on the silica surface.
32409034	6	7	theme	slow	871:874	arg1	release					883:889	a very slow silver release	864:889	a very slow silver release	864:889	They showed good hydration properties and a very slow silver release.
32409034	2	8	theme	solid	318:322	arg1	state					324:328	solid state	318:328	solid state	318:328	First silica supported silver nanoparticles (CAB-O-SIL-Ag) were prepared via solid state sintering route without the use of any solvent and reducing agent.
32409034	3	9	theme	electron	480:487	arg1	microscopy					489:498	transmission electron microscopy	467:498	transmission electron microscopy	467:498	The obtained composite was characterized by X-ray powder diffraction, transmission electron microscopy and UV-vis spectroscopy which evidenced the presence of 8-20 nm spherical silver nanoparticles uniformly distributed and grown on the silica surface.
32409034	3	10	theme	X-ray	441:445	arg1	diffraction					454:464	X-ray powder diffraction	441:464	X-ray powder diffraction	441:464	The obtained composite was characterized by X-ray powder diffraction, transmission electron microscopy and UV-vis spectroscopy which evidenced the presence of 8-20 nm spherical silver nanoparticles uniformly distributed and grown on the silica surface.
32409034	3	11	theme	powder	447:452	arg1	diffraction					454:464	X-ray powder diffraction	441:464	X-ray powder diffraction	441:464	The obtained composite was characterized by X-ray powder diffraction, transmission electron microscopy and UV-vis spectroscopy which evidenced the presence of 8-20 nm spherical silver nanoparticles uniformly distributed and grown on the silica surface.
32409034	7	12	theme	skin	1047:1050	arg1	keratinocytes					1052:1064	human skin keratinocytes	1041:1064	human skin keratinocytes	1041:1064	Films exhibited antimicrobial and antibiofilm activities against Staphylococcus aureus and Pseudomonas aeruginosa and showed no cytotoxicity towards human skin keratinocytes and human fibroblasts HuDe.
32409034	7	13	theme	antibiofilm	926:936	arg1	activities					938:947	antimicrobial and antibiofilm activities	908:947	activities	938:947	Films exhibited antimicrobial and antibiofilm activities against Staphylococcus aureus and Pseudomonas aeruginosa and showed no cytotoxicity towards human skin keratinocytes and human fibroblasts HuDe.
32409034	5	14	with	Films	760:764	arg1	concentrations					792:805	two different silver concentrations	771:805	two different silver concentrations	771:805	Films with two different silver concentrations were prepared.
32409034	0	15	theme	alginate	14:21	arg1	silica					23:28	Biocompatible alginate silica	0:28	Biocompatible alginate silica	0:28	Biocompatible alginate silica supported silver nanoparticles composite films for wound dressing with antibiofilm activity.
32409034	3	16	theme	nanoparticles	581:593	arg1	presence					544:551	the presence	540:551	the presence of 8-20 nm spherical silver nanoparticles uniformly distributed and grown on the silica surface	540:647	The obtained composite was characterized by X-ray powder diffraction, transmission electron microscopy and UV-vis spectroscopy which evidenced the presence of 8-20 nm spherical silver nanoparticles uniformly distributed and grown on the silica surface.
32409034	3	17	theme	silica	634:639	arg1	surface					641:647	the silica surface	630:647	the silica surface	630:647	The obtained composite was characterized by X-ray powder diffraction, transmission electron microscopy and UV-vis spectroscopy which evidenced the presence of 8-20 nm spherical silver nanoparticles uniformly distributed and grown on the silica surface.
32409034	1	18	theme	potential	214:222	arg1	films					132:136	Alginate films	123:136	Alginate films containing pyrogenic silica supported silver nanoparticles	123:195	Alginate films containing pyrogenic silica supported silver nanoparticles were prepared as potential wound dressings.
32409034	1	18	theme	potential	214:222	arg1	dressings					230:238	potential wound dressings	214:238	potential wound dressings	214:238	Alginate films containing pyrogenic silica supported silver nanoparticles were prepared as potential wound dressings.
32409034	0	19	theme	Biocompatible	0:12	arg1	silica					23:28	Biocompatible alginate silica	0:28	Biocompatible alginate silica	0:28	Biocompatible alginate silica supported silver nanoparticles composite films for wound dressing with antibiofilm activity.
32409034	4	20	theme	casting	720:726	arg1	method					728:733	casting method	720:733	casting method	720:733	Then the CAB-O-SIL-Ag was used as filler to prepare alginate films by casting method and successive gelation.
32409034	7	21	theme	antimicrobial	908:920	arg1	activities					938:947	antimicrobial and antibiofilm activities	908:947	activities	938:947	Films exhibited antimicrobial and antibiofilm activities against Staphylococcus aureus and Pseudomonas aeruginosa and showed no cytotoxicity towards human skin keratinocytes and human fibroblasts HuDe.
32409034	1	22	theme	wound	224:228	arg1	films					132:136	Alginate films	123:136	Alginate films containing pyrogenic silica supported silver nanoparticles	123:195	Alginate films containing pyrogenic silica supported silver nanoparticles were prepared as potential wound dressings.
32409034	1	22	theme	wound	224:228	arg1	dressings					230:238	potential wound dressings	214:238	potential wound dressings	214:238	Alginate films containing pyrogenic silica supported silver nanoparticles were prepared as potential wound dressings.
32409034	3	23	theme	obtained	401:408	arg1	composite					410:418	The obtained composite	397:418	The obtained composite	397:418	The obtained composite was characterized by X-ray powder diffraction, transmission electron microscopy and UV-vis spectroscopy which evidenced the presence of 8-20 nm spherical silver nanoparticles uniformly distributed and grown on the silica surface.
32409034	7	24	theme	human	1041:1045	arg1	keratinocytes					1052:1064	human skin keratinocytes	1041:1064	human skin keratinocytes	1041:1064	Films exhibited antimicrobial and antibiofilm activities against Staphylococcus aureus and Pseudomonas aeruginosa and showed no cytotoxicity towards human skin keratinocytes and human fibroblasts HuDe.
32409034	1	25	theme	Alginate	123:130	arg1	films					132:136	Alginate films	123:136	Alginate films containing pyrogenic silica supported silver nanoparticles	123:195	Alginate films containing pyrogenic silica supported silver nanoparticles were prepared as potential wound dressings.
32409034	1	25	theme	Alginate	123:130	arg1	dressings					230:238	potential wound dressings	214:238	potential wound dressings	214:238	Alginate films containing pyrogenic silica supported silver nanoparticles were prepared as potential wound dressings.
32409034	7	26	theme	human	1070:1074	arg1	fibroblasts					1076:1086	human fibroblasts	1070:1086	human fibroblasts HuDe	1070:1091	Films exhibited antimicrobial and antibiofilm activities against Staphylococcus aureus and Pseudomonas aeruginosa and showed no cytotoxicity towards human skin keratinocytes and human fibroblasts HuDe.
32409034	7	26	theme	human	1070:1074	arg1	HuDe					1088:1091	HuDe	1088:1091	HuDe	1088:1091	Films exhibited antimicrobial and antibiofilm activities against Staphylococcus aureus and Pseudomonas aeruginosa and showed no cytotoxicity towards human skin keratinocytes and human fibroblasts HuDe.
32409034	3	27	theme	silver	574:579	arg1	nanoparticles					581:593	8-20 nm spherical silver nanoparticles	556:593	8-20 nm spherical silver nanoparticles uniformly distributed and grown on the silica surface	556:647	The obtained composite was characterized by X-ray powder diffraction, transmission electron microscopy and UV-vis spectroscopy which evidenced the presence of 8-20 nm spherical silver nanoparticles uniformly distributed and grown on the silica surface.
32409034	0	28	theme	nanoparticles	47:59	arg1	films					71:75	silver nanoparticles composite films	40:75	silver nanoparticles composite films for wound dressing with antibiofilm activity	40:120	Biocompatible alginate silica supported silver nanoparticles composite films for wound dressing with antibiofilm activity.
32409034	5	29	theme	different	775:783	arg1	concentrations					792:805	two different silver concentrations	771:805	two different silver concentrations	771:805	Films with two different silver concentrations were prepared.
32409034	5	30	theme	silver	785:790	arg1	concentrations					792:805	two different silver concentrations	771:805	two different silver concentrations	771:805	Films with two different silver concentrations were prepared.
32409034	3	31	theme	transmission	467:478	arg1	microscopy					489:498	transmission electron microscopy	467:498	transmission electron microscopy	467:498	The obtained composite was characterized by X-ray powder diffraction, transmission electron microscopy and UV-vis spectroscopy which evidenced the presence of 8-20 nm spherical silver nanoparticles uniformly distributed and grown on the silica surface.
32409034	0	32	theme	silver	40:45	arg1	films					71:75	silver nanoparticles composite films	40:75	silver nanoparticles composite films for wound dressing with antibiofilm activity	40:120	Biocompatible alginate silica supported silver nanoparticles composite films for wound dressing with antibiofilm activity.
32409034	4	33	used	used	676:679	arg2	filler					684:689	filler	684:689	filler	684:689	Then the CAB-O-SIL-Ag was used as filler to prepare alginate films by casting method and successive gelation.
32409034	4	33	used	used	676:679	arg2	CAB-O-SIL-Ag					659:670	Then the CAB-O-SIL-Ag	650:670	Then the CAB-O-SIL-Ag	650:670	Then the CAB-O-SIL-Ag was used as filler to prepare alginate films by casting method and successive gelation.
32409034	6	34	theme	hydration	839:847	arg1	properties					849:858	good hydration properties	834:858	good hydration properties	834:858	They showed good hydration properties and a very slow silver release.
32409034	3	35	theme	UV-vis	504:509	arg1	spectroscopy					511:522	UV-vis spectroscopy	504:522	UV-vis spectroscopy	504:522	The obtained composite was characterized by X-ray powder diffraction, transmission electron microscopy and UV-vis spectroscopy which evidenced the presence of 8-20 nm spherical silver nanoparticles uniformly distributed and grown on the silica surface.
32409034	2	36	theme	silver	264:269	arg1	nanoparticles					271:283	First silica supported silver nanoparticles	241:283	First silica supported silver nanoparticles (CAB-O-SIL-Ag)	241:298	First silica supported silver nanoparticles (CAB-O-SIL-Ag) were prepared via solid state sintering route without the use of any solvent and reducing agent.
32409034	2	36	theme	silver	264:269	arg1	CAB-O-SIL-Ag					286:297	CAB-O-SIL-Ag	286:297	CAB-O-SIL-Ag	286:297	First silica supported silver nanoparticles (CAB-O-SIL-Ag) were prepared via solid state sintering route without the use of any solvent and reducing agent.
32409034	6	37	theme	good	834:837	arg1	properties					849:858	good hydration properties	834:858	good hydration properties	834:858	They showed good hydration properties and a very slow silver release.
32409034	0	38	theme	composite	61:69	arg1	films					71:75	silver nanoparticles composite films	40:75	silver nanoparticles composite films for wound dressing with antibiofilm activity	40:120	Biocompatible alginate silica supported silver nanoparticles composite films for wound dressing with antibiofilm activity.
32409034	2	39	theme	silica	247:252	arg1	nanoparticles					271:283	First silica supported silver nanoparticles	241:283	First silica supported silver nanoparticles (CAB-O-SIL-Ag)	241:298	First silica supported silver nanoparticles (CAB-O-SIL-Ag) were prepared via solid state sintering route without the use of any solvent and reducing agent.
32409034	2	39	theme	silica	247:252	arg1	CAB-O-SIL-Ag					286:297	CAB-O-SIL-Ag	286:297	CAB-O-SIL-Ag	286:297	First silica supported silver nanoparticles (CAB-O-SIL-Ag) were prepared via solid state sintering route without the use of any solvent and reducing agent.
32409034	2	40	theme	supported	254:262	arg1	nanoparticles					271:283	First silica supported silver nanoparticles	241:283	First silica supported silver nanoparticles (CAB-O-SIL-Ag)	241:298	First silica supported silver nanoparticles (CAB-O-SIL-Ag) were prepared via solid state sintering route without the use of any solvent and reducing agent.
32409034	2	40	theme	supported	254:262	arg1	CAB-O-SIL-Ag					286:297	CAB-O-SIL-Ag	286:297	CAB-O-SIL-Ag	286:297	First silica supported silver nanoparticles (CAB-O-SIL-Ag) were prepared via solid state sintering route without the use of any solvent and reducing agent.
32409034	3	41	theme	spherical	564:572	arg1	nanoparticles					581:593	8-20 nm spherical silver nanoparticles	556:593	8-20 nm spherical silver nanoparticles uniformly distributed and grown on the silica surface	556:647	The obtained composite was characterized by X-ray powder diffraction, transmission electron microscopy and UV-vis spectroscopy which evidenced the presence of 8-20 nm spherical silver nanoparticles uniformly distributed and grown on the silica surface.
32409034	2	42	theme	solvent	369:375	arg1	agent					390:394	any solvent and reducing agent	365:394	agent	390:394	First silica supported silver nanoparticles (CAB-O-SIL-Ag) were prepared via solid state sintering route without the use of any solvent and reducing agent.
32409034	2	43	theme	First	241:245	arg1	nanoparticles					271:283	First silica supported silver nanoparticles	241:283	First silica supported silver nanoparticles (CAB-O-SIL-Ag)	241:298	First silica supported silver nanoparticles (CAB-O-SIL-Ag) were prepared via solid state sintering route without the use of any solvent and reducing agent.
32409034	2	43	theme	First	241:245	arg1	CAB-O-SIL-Ag					286:297	CAB-O-SIL-Ag	286:297	CAB-O-SIL-Ag	286:297	First silica supported silver nanoparticles (CAB-O-SIL-Ag) were prepared via solid state sintering route without the use of any solvent and reducing agent.
32409034	4	44	theme	successive	739:748	arg1	gelation					750:757	successive gelation	739:757	successive gelation	739:757	Then the CAB-O-SIL-Ag was used as filler to prepare alginate films by casting method and successive gelation.
32409034	1	45	theme	pyrogenic	149:157	arg1	nanoparticles					183:195	pyrogenic silica supported silver nanoparticles	149:195	pyrogenic silica supported silver nanoparticles	149:195	Alginate films containing pyrogenic silica supported silver nanoparticles were prepared as potential wound dressings.
32409034	0	46	with	films	71:75	arg1	activity					113:120	antibiofilm activity	101:120	antibiofilm activity	101:120	Biocompatible alginate silica supported silver nanoparticles composite films for wound dressing with antibiofilm activity.
32409034	1	47	theme	silica	159:164	arg1	nanoparticles					183:195	pyrogenic silica supported silver nanoparticles	149:195	pyrogenic silica supported silver nanoparticles	149:195	Alginate films containing pyrogenic silica supported silver nanoparticles were prepared as potential wound dressings.
32409034	0	48	theme	wound	81:85	arg1	dressing					87:94	wound dressing	81:94	wound dressing	81:94	Biocompatible alginate silica supported silver nanoparticles composite films for wound dressing with antibiofilm activity.
32409034	4	49	theme	alginate	702:709	arg1	films					711:715	alginate films	702:715	alginate films	702:715	Then the CAB-O-SIL-Ag was used as filler to prepare alginate films by casting method and successive gelation.
32409034	1	50	theme	supported	166:174	arg1	nanoparticles					183:195	pyrogenic silica supported silver nanoparticles	149:195	pyrogenic silica supported silver nanoparticles	149:195	Alginate films containing pyrogenic silica supported silver nanoparticles were prepared as potential wound dressings.
32409034	1	51	theme	silver	176:181	arg1	nanoparticles					183:195	pyrogenic silica supported silver nanoparticles	149:195	pyrogenic silica supported silver nanoparticles	149:195	Alginate films containing pyrogenic silica supported silver nanoparticles were prepared as potential wound dressings.
33807914	7	0	theme	Muc2	1204:1207	arg1	mice					1209:1212	Muc2 mice	1204:1212	Muc2 mice	1204:1212	We propose that the changes in beneficial microflora affect the immune status in Muc2 mice, which causes implantation impairment.
33807914	2	1	theme	system	380:385	arg1	reprogramming					387:399	mucosal immune system reprogramming	365:399	mucosal immune system reprogramming	365:399	A lack of intestinal protective mucus results in changes in the commensal microflora composition, mucosal immune system reprogramming, and inflammation.
33807914	6	2	theme	wild-type	986:994	arg1	siblings					996:1003	the otherwise healthy wild-type siblings	964:1003	the otherwise healthy wild-type siblings of Muc2 animals	964:1019	Metagenomic analysis reveals that the otherwise healthy wild-type siblings of Muc2 animals have reduced numbers of some of the intestinal commensal bacterial species, compared to C57BL/6 mice.
33807914	7	3	from	changes	1143:1149	arg1	microflora					1165:1174	beneficial microflora	1154:1174	beneficial microflora	1154:1174	We propose that the changes in beneficial microflora affect the immune status in Muc2 mice, which causes implantation impairment.
33807914	9	4	theme	oxidative	1530:1538	arg1	phosphorylation					1540:1554	oxidative phosphorylation	1530:1554	oxidative phosphorylation	1530:1554	Metabolic profiling of peritoneal macrophages from Muc2 females reveals their predisposition towards anaerobic glycolysis in favor of oxidative phosphorylation, compared to C57BL/6-derived cells.
33807914	4	5	theme	heterozygous	700:711	arg1	mice					732:735	heterozygous mutant Muc2 female mice	700:735	heterozygous mutant Muc2 female mice	700:735	This study evaluates the effect of fucose on reproductive performance in heterozygous mutant Muc2 female mice.
33807914	10	6	theme	oxidative	1696:1704	arg1	phosphorylation					1706:1720	oxidative phosphorylation	1696:1720	oxidative phosphorylation	1696:1720	In vitro experiments on phagocytosis activity and mitochondrial respiration suggest that fucose affects oxidative phosphorylation in a genotype-specific manner, which might interfere with implantation depending on the initial status of macrophages.
33807914	13	7	theme	same	2114:2117	arg1	time					2119:2122	the same time	2110:2122	the same time	2110:2122	At the same time, dietary fucose might act as a differential regulator of macrophage polarization during implantation, depending on the immune status of the host.
33807914	5	8	theme	reduced	860:866	arg1	performance					881:891	a significantly reduced reproductive performance	844:891	a significantly reduced reproductive performance upon dietary fucose supplementation	844:927	We found that even though Muc2 females are physiologically indistinguishable from C57Bl/6 mice, they have a significantly reduced reproductive performance upon dietary fucose supplementation.
33807914	4	9	theme	Muc2	720:723	arg1	mice					732:735	heterozygous mutant Muc2 female mice	700:735	heterozygous mutant Muc2 female mice	700:735	This study evaluates the effect of fucose on reproductive performance in heterozygous mutant Muc2 female mice.
33807914	11	10	theme	M2	1972:1973	arg1	polarization					1986:1997	opposed implantation-associated M2 macrophage polarization	1940:1997	opposed implantation-associated M2 macrophage polarization	1940:1997	This hypothesis is further confirmed in BALB/c female mice, where fucose caused pregnancy loss and opposed implantation-associated M2 macrophage polarization.
33807914	2	11	theme	commensal	331:339	arg1	composition					352:362	the commensal microflora composition	327:362	the commensal microflora composition	327:362	A lack of intestinal protective mucus results in changes in the commensal microflora composition, mucosal immune system reprogramming, and inflammation.
33807914	4	12	from	performance	685:695	arg1	mice					732:735	heterozygous mutant Muc2 female mice	700:735	heterozygous mutant Muc2 female mice	700:735	This study evaluates the effect of fucose on reproductive performance in heterozygous mutant Muc2 female mice.
33807914	10	13	theme	genotype-specific	1727:1743	arg1	manner					1745:1750	a genotype-specific manner	1725:1750	a genotype-specific manner	1725:1750	In vitro experiments on phagocytosis activity and mitochondrial respiration suggest that fucose affects oxidative phosphorylation in a genotype-specific manner, which might interfere with implantation depending on the initial status of macrophages.
33807914	11	14	theme	opposed	1940:1946	arg1	polarization					1986:1997	opposed implantation-associated M2 macrophage polarization	1940:1997	opposed implantation-associated M2 macrophage polarization	1940:1997	This hypothesis is further confirmed in BALB/c female mice, where fucose caused pregnancy loss and opposed implantation-associated M2 macrophage polarization.
33807914	3	15	theme	mouse	602:606	arg1	models					608:613	some mouse models	597:613	some mouse models of colitis	597:624	Previous work has shown that fucose, the terminal glycan chain component of the intestinal glycoprotein Mucin2, and fucoidan polysaccharides have an anti-inflammatory effect in some mouse models of colitis.
33807914	5	16	theme	dietary	898:904	arg1	supplementation					913:927	dietary fucose supplementation	898:927	dietary fucose supplementation	898:927	We found that even though Muc2 females are physiologically indistinguishable from C57Bl/6 mice, they have a significantly reduced reproductive performance upon dietary fucose supplementation.
33807914	8	17	theme	Muc2	1358:1361	arg1	females					1363:1369	Muc2 females	1358:1369	Muc2 females	1358:1369	In accordance with this hypothesis, we find that macrophage polarization during pregnancy is impaired in Muc2 females upon addition of fucose.
33807914	0	18	theme	Macrophage	23:32	arg1	Polarization					34:45	Macrophage Polarization	23:45	Macrophage Polarization	23:45	Dietary Fucose Affects Macrophage Polarization and Reproductive Performance in Mice.
33807914	5	19	theme	reproductive	868:879	arg1	performance					881:891	a significantly reduced reproductive performance	844:891	a significantly reduced reproductive performance upon dietary fucose supplementation	844:927	We found that even though Muc2 females are physiologically indistinguishable from C57Bl/6 mice, they have a significantly reduced reproductive performance upon dietary fucose supplementation.
33807914	6	20	theme	reduced	1026:1032	arg1	numbers					1034:1040	reduced numbers	1026:1040	reduced numbers of some of the intestinal commensal bacterial species	1026:1094	Metagenomic analysis reveals that the otherwise healthy wild-type siblings of Muc2 animals have reduced numbers of some of the intestinal commensal bacterial species, compared to C57BL/6 mice.
33807914	12	21	theme	intestinal	2040:2049	arg1	microflora					2051:2060	intestinal microflora	2040:2060	intestinal microflora	2040:2060	Taken together, these data suggest that intestinal microflora affects host immunity and pregnancy outcome.
33807914	13	22	theme	host	2264:2267	arg1	status					2250:2255	the immune status	2239:2255	the immune status of the host	2239:2267	At the same time, dietary fucose might act as a differential regulator of macrophage polarization during implantation, depending on the immune status of the host.
33807914	6	23	theme	animals	1013:1019	arg1	siblings					996:1003	the otherwise healthy wild-type siblings	964:1003	the otherwise healthy wild-type siblings of Muc2 animals	964:1019	Metagenomic analysis reveals that the otherwise healthy wild-type siblings of Muc2 animals have reduced numbers of some of the intestinal commensal bacterial species, compared to C57BL/6 mice.
33807914	5	24	contain	have	839:842	arg2	performance					881:891	a significantly reduced reproductive performance	844:891	a significantly reduced reproductive performance upon dietary fucose supplementation	844:927	We found that even though Muc2 females are physiologically indistinguishable from C57Bl/6 mice, they have a significantly reduced reproductive performance upon dietary fucose supplementation.
33807914	5	24	contain	have	839:842	arg1	they					834:837	they	834:837	they	834:837	We found that even though Muc2 females are physiologically indistinguishable from C57Bl/6 mice, they have a significantly reduced reproductive performance upon dietary fucose supplementation.
33807914	2	25	theme	intestinal	277:286	arg1	mucus					299:303	intestinal protective mucus	277:303	intestinal protective mucus	277:303	A lack of intestinal protective mucus results in changes in the commensal microflora composition, mucosal immune system reprogramming, and inflammation.
33807914	2	26	from	changes	316:322	arg1	reprogramming					387:399	mucosal immune system reprogramming	365:399	mucosal immune system reprogramming	365:399	A lack of intestinal protective mucus results in changes in the commensal microflora composition, mucosal immune system reprogramming, and inflammation.
33807914	2	26	from	changes	316:322	arg1	inflammation					406:417	inflammation	406:417	inflammation	406:417	A lack of intestinal protective mucus results in changes in the commensal microflora composition, mucosal immune system reprogramming, and inflammation.
33807914	2	26	from	changes	316:322	arg1	composition					352:362	the commensal microflora composition	327:362	the commensal microflora composition	327:362	A lack of intestinal protective mucus results in changes in the commensal microflora composition, mucosal immune system reprogramming, and inflammation.
33807914	4	27	from	effect	652:657	arg1	performance					685:695	reproductive performance	672:695	reproductive performance in heterozygous mutant Muc2 female mice	672:735	This study evaluates the effect of fucose on reproductive performance in heterozygous mutant Muc2 female mice.
33807914	13	28	theme	immune	2243:2248	arg1	status					2250:2255	the immune status	2239:2255	the immune status of the host	2239:2267	At the same time, dietary fucose might act as a differential regulator of macrophage polarization during implantation, depending on the immune status of the host.
33807914	1	29	theme	resident	152:159	arg1	microorganisms					161:174	the gut resident microorganisms	144:174	the gut resident microorganisms	144:174	Intestinal mucus protects epithelial and immune cells from the gut resident microorganisms, and provides growth-promoting factors as mucus-derived O-glycans for beneficial bacteria.
33807914	1	30	from	microorganisms	161:174	arg1	cells					133:137	epithelial and immune cells	111:137	epithelial and immune cells from the gut resident microorganisms	111:174	Intestinal mucus protects epithelial and immune cells from the gut resident microorganisms, and provides growth-promoting factors as mucus-derived O-glycans for beneficial bacteria.
33807914	6	31	theme	bacterial	1078:1086	arg1	species					1088:1094	the intestinal commensal bacterial species	1053:1094	the intestinal commensal bacterial species	1053:1094	Metagenomic analysis reveals that the otherwise healthy wild-type siblings of Muc2 animals have reduced numbers of some of the intestinal commensal bacterial species, compared to C57BL/6 mice.
33807914	9	32	from	females	1452:1458	arg1	macrophages					1430:1440	peritoneal macrophages	1419:1440	peritoneal macrophages from Muc2 females	1419:1458	Metabolic profiling of peritoneal macrophages from Muc2 females reveals their predisposition towards anaerobic glycolysis in favor of oxidative phosphorylation, compared to C57BL/6-derived cells.
33807914	9	32	from	females	1452:1458	arg1	profiling					1406:1414	Metabolic profiling	1396:1414	Metabolic profiling of peritoneal macrophages from Muc2 females	1396:1458	Metabolic profiling of peritoneal macrophages from Muc2 females reveals their predisposition towards anaerobic glycolysis in favor of oxidative phosphorylation, compared to C57BL/6-derived cells.
33807914	6	33	theme	intestinal	1057:1066	arg1	species					1088:1094	the intestinal commensal bacterial species	1053:1094	the intestinal commensal bacterial species	1053:1094	Metagenomic analysis reveals that the otherwise healthy wild-type siblings of Muc2 animals have reduced numbers of some of the intestinal commensal bacterial species, compared to C57BL/6 mice.
33807914	10	34	theme	In	1592:1593	arg1	experiments					1601:1611	In vitro experiments	1592:1611	In vitro experiments on phagocytosis activity and mitochondrial respiration	1592:1666	In vitro experiments on phagocytosis activity and mitochondrial respiration suggest that fucose affects oxidative phosphorylation in a genotype-specific manner, which might interfere with implantation depending on the initial status of macrophages.
33807914	3	35	theme	terminal	461:468	arg1	component					483:491	the terminal glycan chain component	457:491	the terminal glycan chain component of the intestinal glycoprotein Mucin2	457:529	Previous work has shown that fucose, the terminal glycan chain component of the intestinal glycoprotein Mucin2, and fucoidan polysaccharides have an anti-inflammatory effect in some mouse models of colitis.
33807914	3	35	theme	terminal	461:468	arg1	fucose					449:454	fucose	449:454	fucose	449:454	Previous work has shown that fucose, the terminal glycan chain component of the intestinal glycoprotein Mucin2, and fucoidan polysaccharides have an anti-inflammatory effect in some mouse models of colitis.
33807914	9	36	theme	Metabolic	1396:1404	arg1	profiling					1406:1414	Metabolic profiling	1396:1414	Metabolic profiling of peritoneal macrophages from Muc2 females	1396:1458	Metabolic profiling of peritoneal macrophages from Muc2 females reveals their predisposition towards anaerobic glycolysis in favor of oxidative phosphorylation, compared to C57BL/6-derived cells.
33807914	12	37	theme	host	2070:2073	arg1	immunity					2075:2082	host immunity	2070:2082	host immunity	2070:2082	Taken together, these data suggest that intestinal microflora affects host immunity and pregnancy outcome.
33807914	1	38	theme	Intestinal	85:94	arg1	mucus					96:100	Intestinal mucus	85:100	Intestinal mucus	85:100	Intestinal mucus protects epithelial and immune cells from the gut resident microorganisms, and provides growth-promoting factors as mucus-derived O-glycans for beneficial bacteria.
33807914	3	39	theme	Previous	420:427	arg1	work					429:432	Previous work	420:432	Previous work	420:432	Previous work has shown that fucose, the terminal glycan chain component of the intestinal glycoprotein Mucin2, and fucoidan polysaccharides have an anti-inflammatory effect in some mouse models of colitis.
33807914	0	40	from	Polarization	34:45	arg1	Mice					79:82	Mice	79:82	Mice	79:82	Dietary Fucose Affects Macrophage Polarization and Reproductive Performance in Mice.
33807914	0	41	theme	Dietary	0:6	arg1	Fucose					8:13	Dietary Fucose	0:13	Dietary Fucose	0:13	Dietary Fucose Affects Macrophage Polarization and Reproductive Performance in Mice.
33807914	10	42	theme	phagocytosis	1616:1627	arg1	activity					1629:1636	phagocytosis activity	1616:1636	phagocytosis activity	1616:1636	In vitro experiments on phagocytosis activity and mitochondrial respiration suggest that fucose affects oxidative phosphorylation in a genotype-specific manner, which might interfere with implantation depending on the initial status of macrophages.
33807914	3	43	gly	glycoprotein	511:522	arg1	glycoprotein					511:522	the intestinal glycoprotein Mucin2	496:529	the intestinal glycoprotein Mucin2	496:529	Previous work has shown that fucose, the terminal glycan chain component of the intestinal glycoprotein Mucin2, and fucoidan polysaccharides have an anti-inflammatory effect in some mouse models of colitis.
33807914	9	44	theme	macrophages	1430:1440	arg1	profiling					1406:1414	Metabolic profiling	1396:1414	Metabolic profiling of peritoneal macrophages from Muc2 females	1396:1458	Metabolic profiling of peritoneal macrophages from Muc2 females reveals their predisposition towards anaerobic glycolysis in favor of oxidative phosphorylation, compared to C57BL/6-derived cells.
33807914	3	45	theme	chain	477:481	arg1	component					483:491	the terminal glycan chain component	457:491	the terminal glycan chain component of the intestinal glycoprotein Mucin2	457:529	Previous work has shown that fucose, the terminal glycan chain component of the intestinal glycoprotein Mucin2, and fucoidan polysaccharides have an anti-inflammatory effect in some mouse models of colitis.
33807914	3	45	theme	chain	477:481	arg1	fucose					449:454	fucose	449:454	fucose	449:454	Previous work has shown that fucose, the terminal glycan chain component of the intestinal glycoprotein Mucin2, and fucoidan polysaccharides have an anti-inflammatory effect in some mouse models of colitis.
33807914	9	46	theme	Muc2	1447:1450	arg1	females					1452:1458	Muc2 females	1447:1458	Muc2 females	1447:1458	Metabolic profiling of peritoneal macrophages from Muc2 females reveals their predisposition towards anaerobic glycolysis in favor of oxidative phosphorylation, compared to C57BL/6-derived cells.
33807914	6	47	theme	Metagenomic	930:940	arg1	analysis					942:949	Metagenomic analysis	930:949	Metagenomic analysis	930:949	Metagenomic analysis reveals that the otherwise healthy wild-type siblings of Muc2 animals have reduced numbers of some of the intestinal commensal bacterial species, compared to C57BL/6 mice.
33807914	7	48	from	status	1194:1199	arg1	mice					1209:1212	Muc2 mice	1204:1212	Muc2 mice	1204:1212	We propose that the changes in beneficial microflora affect the immune status in Muc2 mice, which causes implantation impairment.
33807914	13	49	theme	polarization	2192:2203	arg1	regulator					2168:2176	a differential regulator	2153:2176	a differential regulator of macrophage polarization during implantation	2153:2223	At the same time, dietary fucose might act as a differential regulator of macrophage polarization during implantation, depending on the immune status of the host.
33807914	13	49	theme	polarization	2192:2203	arg1	fucose					2133:2138	dietary fucose	2125:2138	dietary fucose	2125:2138	At the same time, dietary fucose might act as a differential regulator of macrophage polarization during implantation, depending on the immune status of the host.
33807914	3	50	theme	Mucin2	524:529	arg1	component					483:491	the terminal glycan chain component	457:491	the terminal glycan chain component of the intestinal glycoprotein Mucin2	457:529	Previous work has shown that fucose, the terminal glycan chain component of the intestinal glycoprotein Mucin2, and fucoidan polysaccharides have an anti-inflammatory effect in some mouse models of colitis.
33807914	3	50	theme	Mucin2	524:529	arg1	fucose					449:454	fucose	449:454	fucose	449:454	Previous work has shown that fucose, the terminal glycan chain component of the intestinal glycoprotein Mucin2, and fucoidan polysaccharides have an anti-inflammatory effect in some mouse models of colitis.
33807914	0	51	theme	Reproductive	51:62	arg1	Performance					64:74	Reproductive Performance	51:74	Reproductive Performance	51:74	Dietary Fucose Affects Macrophage Polarization and Reproductive Performance in Mice.
33807914	9	52	from	glycolysis	1507:1516	arg1	favor					1521:1525	favor	1521:1525	favor	1521:1525	Metabolic profiling of peritoneal macrophages from Muc2 females reveals their predisposition towards anaerobic glycolysis in favor of oxidative phosphorylation, compared to C57BL/6-derived cells.
33807914	10	53	from	phosphorylation	1706:1720	arg1	manner					1745:1750	a genotype-specific manner	1725:1750	a genotype-specific manner	1725:1750	In vitro experiments on phagocytosis activity and mitochondrial respiration suggest that fucose affects oxidative phosphorylation in a genotype-specific manner, which might interfere with implantation depending on the initial status of macrophages.
33807914	11	54	theme	female	1888:1893	arg1	mice					1895:1898	BALB/c female mice	1881:1898	BALB/c female mice	1881:1898	This hypothesis is further confirmed in BALB/c female mice, where fucose caused pregnancy loss and opposed implantation-associated M2 macrophage polarization.
33807914	3	55	theme	intestinal	500:509	arg1	Mucin2					524:529	the intestinal glycoprotein Mucin2	496:529	the intestinal glycoprotein Mucin2	496:529	Previous work has shown that fucose, the terminal glycan chain component of the intestinal glycoprotein Mucin2, and fucoidan polysaccharides have an anti-inflammatory effect in some mouse models of colitis.
33807914	1	56	theme	beneficial	246:255	arg1	bacteria					257:264	beneficial bacteria	246:264	beneficial bacteria	246:264	Intestinal mucus protects epithelial and immune cells from the gut resident microorganisms, and provides growth-promoting factors as mucus-derived O-glycans for beneficial bacteria.
33807914	3	57	theme	anti-inflammatory	569:585	arg1	effect					587:592	an anti-inflammatory effect	566:592	an anti-inflammatory effect	566:592	Previous work has shown that fucose, the terminal glycan chain component of the intestinal glycoprotein Mucin2, and fucoidan polysaccharides have an anti-inflammatory effect in some mouse models of colitis.
33807914	4	58	theme	female	725:730	arg1	mice					732:735	heterozygous mutant Muc2 female mice	700:735	heterozygous mutant Muc2 female mice	700:735	This study evaluates the effect of fucose on reproductive performance in heterozygous mutant Muc2 female mice.
33807914	13	59	theme	differential	2155:2166	arg1	regulator					2168:2176	a differential regulator	2153:2176	a differential regulator of macrophage polarization during implantation	2153:2223	At the same time, dietary fucose might act as a differential regulator of macrophage polarization during implantation, depending on the immune status of the host.
33807914	13	59	theme	differential	2155:2166	arg1	fucose					2133:2138	dietary fucose	2125:2138	dietary fucose	2125:2138	At the same time, dietary fucose might act as a differential regulator of macrophage polarization during implantation, depending on the immune status of the host.
33807914	9	60	theme	anaerobic	1497:1505	arg1	glycolysis					1507:1516	anaerobic glycolysis	1497:1516	anaerobic glycolysis in favor of oxidative phosphorylation	1497:1554	Metabolic profiling of peritoneal macrophages from Muc2 females reveals their predisposition towards anaerobic glycolysis in favor of oxidative phosphorylation, compared to C57BL/6-derived cells.
33807914	6	61	theme	Muc2	1008:1011	arg1	animals					1013:1019	Muc2 animals	1008:1019	Muc2 animals	1008:1019	Metagenomic analysis reveals that the otherwise healthy wild-type siblings of Muc2 animals have reduced numbers of some of the intestinal commensal bacterial species, compared to C57BL/6 mice.
33807914	7	62	theme	immune	1187:1192	arg1	status					1194:1199	the immune status	1183:1199	the immune status	1183:1199	We propose that the changes in beneficial microflora affect the immune status in Muc2 mice, which causes implantation impairment.
33807914	6	63	theme	healthy	978:984	arg1	siblings					996:1003	the otherwise healthy wild-type siblings	964:1003	the otherwise healthy wild-type siblings of Muc2 animals	964:1019	Metagenomic analysis reveals that the otherwise healthy wild-type siblings of Muc2 animals have reduced numbers of some of the intestinal commensal bacterial species, compared to C57BL/6 mice.
33807914	2	64	theme	immune	373:378	arg1	system					380:385	mucosal immune system	365:385	mucosal immune system reprogramming	365:399	A lack of intestinal protective mucus results in changes in the commensal microflora composition, mucosal immune system reprogramming, and inflammation.
33807914	8	65	theme	macrophage	1302:1311	arg1	polarization					1313:1324	macrophage polarization	1302:1324	macrophage polarization during pregnancy	1302:1341	In accordance with this hypothesis, we find that macrophage polarization during pregnancy is impaired in Muc2 females upon addition of fucose.
33807914	7	66	theme	beneficial	1154:1163	arg1	microflora					1165:1174	beneficial microflora	1154:1174	beneficial microflora	1154:1174	We propose that the changes in beneficial microflora affect the immune status in Muc2 mice, which causes implantation impairment.
33807914	2	67	theme	microflora	341:350	arg1	composition					352:362	the commensal microflora composition	327:362	the commensal microflora composition	327:362	A lack of intestinal protective mucus results in changes in the commensal microflora composition, mucosal immune system reprogramming, and inflammation.
33807914	1	68	theme	growth-promoting	190:205	arg1	factors					207:213	growth-promoting factors	190:213	growth-promoting factors as mucus-derived O-glycans for beneficial bacteria	190:264	Intestinal mucus protects epithelial and immune cells from the gut resident microorganisms, and provides growth-promoting factors as mucus-derived O-glycans for beneficial bacteria.
33807914	9	69	from	profiling	1406:1414	arg1	females					1452:1458	Muc2 females	1447:1458	Muc2 females	1447:1458	Metabolic profiling of peritoneal macrophages from Muc2 females reveals their predisposition towards anaerobic glycolysis in favor of oxidative phosphorylation, compared to C57BL/6-derived cells.
33807914	4	70	theme	mutant	713:718	arg1	mice					732:735	heterozygous mutant Muc2 female mice	700:735	heterozygous mutant Muc2 female mice	700:735	This study evaluates the effect of fucose on reproductive performance in heterozygous mutant Muc2 female mice.
33807914	11	71	theme	macrophage	1975:1984	arg1	polarization					1986:1997	opposed implantation-associated M2 macrophage polarization	1940:1997	opposed implantation-associated M2 macrophage polarization	1940:1997	This hypothesis is further confirmed in BALB/c female mice, where fucose caused pregnancy loss and opposed implantation-associated M2 macrophage polarization.
33807914	13	72	theme	dietary	2125:2131	arg1	regulator					2168:2176	a differential regulator	2153:2176	a differential regulator of macrophage polarization during implantation	2153:2223	At the same time, dietary fucose might act as a differential regulator of macrophage polarization during implantation, depending on the immune status of the host.
33807914	13	72	theme	dietary	2125:2131	arg1	fucose					2133:2138	dietary fucose	2125:2138	dietary fucose	2125:2138	At the same time, dietary fucose might act as a differential regulator of macrophage polarization during implantation, depending on the immune status of the host.
33807914	9	73	theme	C57BL/6-derived	1569:1583	arg1	cells					1585:1589	C57BL/6-derived cells	1569:1589	C57BL/6-derived cells	1569:1589	Metabolic profiling of peritoneal macrophages from Muc2 females reveals their predisposition towards anaerobic glycolysis in favor of oxidative phosphorylation, compared to C57BL/6-derived cells.
33807914	6	74	theme	some	1045:1048	arg1	numbers					1034:1040	reduced numbers	1026:1040	reduced numbers of some of the intestinal commensal bacterial species	1026:1094	Metagenomic analysis reveals that the otherwise healthy wild-type siblings of Muc2 animals have reduced numbers of some of the intestinal commensal bacterial species, compared to C57BL/6 mice.
33807914	10	75	dep	In	1592:1593	arg1	vitro					1595:1599	vitro	1595:1599	vitro	1595:1599	In vitro experiments on phagocytosis activity and mitochondrial respiration suggest that fucose affects oxidative phosphorylation in a genotype-specific manner, which might interfere with implantation depending on the initial status of macrophages.
33807914	4	76	theme	fucose	662:667	arg1	effect					652:657	the effect	648:657	the effect of fucose on reproductive performance in heterozygous mutant Muc2 female mice	648:735	This study evaluates the effect of fucose on reproductive performance in heterozygous mutant Muc2 female mice.
33807914	11	77	theme	implantation-associated	1948:1970	arg1	polarization					1986:1997	opposed implantation-associated M2 macrophage polarization	1940:1997	opposed implantation-associated M2 macrophage polarization	1940:1997	This hypothesis is further confirmed in BALB/c female mice, where fucose caused pregnancy loss and opposed implantation-associated M2 macrophage polarization.
33807914	5	78	theme	fucose	906:911	arg1	supplementation					913:927	dietary fucose supplementation	898:927	dietary fucose supplementation	898:927	We found that even though Muc2 females are physiologically indistinguishable from C57Bl/6 mice, they have a significantly reduced reproductive performance upon dietary fucose supplementation.
33807914	4	79	theme	reproductive	672:683	arg1	performance					685:695	reproductive performance	672:695	reproductive performance in heterozygous mutant Muc2 female mice	672:735	This study evaluates the effect of fucose on reproductive performance in heterozygous mutant Muc2 female mice.
33807914	3	80	theme	colitis	618:624	arg1	models					608:613	some mouse models	597:613	some mouse models of colitis	597:624	Previous work has shown that fucose, the terminal glycan chain component of the intestinal glycoprotein Mucin2, and fucoidan polysaccharides have an anti-inflammatory effect in some mouse models of colitis.
33807914	1	81	theme	immune	126:131	arg1	cells					133:137	epithelial and immune cells	111:137	epithelial and immune cells from the gut resident microorganisms	111:174	Intestinal mucus protects epithelial and immune cells from the gut resident microorganisms, and provides growth-promoting factors as mucus-derived O-glycans for beneficial bacteria.
33807914	5	82	from	mice	828:831	arg1	indistinguishable					797:813	indistinguishable	797:813	indistinguishable	797:813	We found that even though Muc2 females are physiologically indistinguishable from C57Bl/6 mice, they have a significantly reduced reproductive performance upon dietary fucose supplementation.
33807914	2	83	theme	protective	288:297	arg1	mucus					299:303	intestinal protective mucus	277:303	intestinal protective mucus	277:303	A lack of intestinal protective mucus results in changes in the commensal microflora composition, mucosal immune system reprogramming, and inflammation.
33807914	6	84	theme	C57BL/6	1109:1115	arg1	mice					1117:1120	C57BL/6 mice	1109:1120	C57BL/6 mice	1109:1120	Metagenomic analysis reveals that the otherwise healthy wild-type siblings of Muc2 animals have reduced numbers of some of the intestinal commensal bacterial species, compared to C57BL/6 mice.
33807914	1	85	theme	gut	148:150	arg1	microorganisms					161:174	the gut resident microorganisms	144:174	the gut resident microorganisms	144:174	Intestinal mucus protects epithelial and immune cells from the gut resident microorganisms, and provides growth-promoting factors as mucus-derived O-glycans for beneficial bacteria.
33807914	2	86	theme	mucus	299:303	arg1	lack					269:272	A lack	267:272	A lack of intestinal protective mucus	267:303	A lack of intestinal protective mucus results in changes in the commensal microflora composition, mucosal immune system reprogramming, and inflammation.
33807914	8	87	theme	fucose	1388:1393	arg1	addition					1376:1383	addition	1376:1383	addition of fucose	1376:1393	In accordance with this hypothesis, we find that macrophage polarization during pregnancy is impaired in Muc2 females upon addition of fucose.
33807914	5	88	theme	C57Bl/6	820:826	arg1	mice					828:831	C57Bl/6 mice	820:831	C57Bl/6 mice	820:831	We found that even though Muc2 females are physiologically indistinguishable from C57Bl/6 mice, they have a significantly reduced reproductive performance upon dietary fucose supplementation.
33807914	3	89	contain	have	561:564	arg1	component					483:491	the terminal glycan chain component	457:491	the terminal glycan chain component of the intestinal glycoprotein Mucin2	457:529	Previous work has shown that fucose, the terminal glycan chain component of the intestinal glycoprotein Mucin2, and fucoidan polysaccharides have an anti-inflammatory effect in some mouse models of colitis.
33807914	3	89	contain	have	561:564	arg1	fucoidan					536:543	fucoidan	536:543	fucoidan	536:543	Previous work has shown that fucose, the terminal glycan chain component of the intestinal glycoprotein Mucin2, and fucoidan polysaccharides have an anti-inflammatory effect in some mouse models of colitis.
33807914	3	89	contain	have	561:564	arg1	fucose					449:454	fucose	449:454	fucose	449:454	Previous work has shown that fucose, the terminal glycan chain component of the intestinal glycoprotein Mucin2, and fucoidan polysaccharides have an anti-inflammatory effect in some mouse models of colitis.
33807914	3	89	contain	have	561:564	arg2	effect					587:592	an anti-inflammatory effect	566:592	an anti-inflammatory effect	566:592	Previous work has shown that fucose, the terminal glycan chain component of the intestinal glycoprotein Mucin2, and fucoidan polysaccharides have an anti-inflammatory effect in some mouse models of colitis.
33807914	5	90	theme	Muc2	764:767	arg1	females					769:775	Muc2 females	764:775	Muc2 females	764:775	We found that even though Muc2 females are physiologically indistinguishable from C57Bl/6 mice, they have a significantly reduced reproductive performance upon dietary fucose supplementation.
33807914	9	91	link	C57BL/6-derived	1569:1583	arg1	cells					1585:1589	C57BL/6-derived cells	1569:1589	C57BL/6-derived cells	1569:1589	Metabolic profiling of peritoneal macrophages from Muc2 females reveals their predisposition towards anaerobic glycolysis in favor of oxidative phosphorylation, compared to C57BL/6-derived cells.
33807914	6	92	theme	commensal	1068:1076	arg1	species					1088:1094	the intestinal commensal bacterial species	1053:1094	the intestinal commensal bacterial species	1053:1094	Metagenomic analysis reveals that the otherwise healthy wild-type siblings of Muc2 animals have reduced numbers of some of the intestinal commensal bacterial species, compared to C57BL/6 mice.
33807914	0	93	from	Performance	64:74	arg1	Mice					79:82	Mice	79:82	Mice	79:82	Dietary Fucose Affects Macrophage Polarization and Reproductive Performance in Mice.
33807914	1	94	link	mucus-derived	218:230	arg1	O-glycans					232:240	mucus-derived O-glycans	218:240	mucus-derived O-glycans	218:240	Intestinal mucus protects epithelial and immune cells from the gut resident microorganisms, and provides growth-promoting factors as mucus-derived O-glycans for beneficial bacteria.
33807914	11	95	theme	pregnancy	1921:1929	arg1	loss					1931:1934	pregnancy loss	1921:1934	pregnancy loss	1921:1934	This hypothesis is further confirmed in BALB/c female mice, where fucose caused pregnancy loss and opposed implantation-associated M2 macrophage polarization.
33807914	1	96	theme	mucus-derived	218:230	arg1	O-glycans					232:240	mucus-derived O-glycans	218:240	mucus-derived O-glycans	218:240	Intestinal mucus protects epithelial and immune cells from the gut resident microorganisms, and provides growth-promoting factors as mucus-derived O-glycans for beneficial bacteria.
33807914	3	97	theme	glycan	470:475	arg1	component					483:491	the terminal glycan chain component	457:491	the terminal glycan chain component of the intestinal glycoprotein Mucin2	457:529	Previous work has shown that fucose, the terminal glycan chain component of the intestinal glycoprotein Mucin2, and fucoidan polysaccharides have an anti-inflammatory effect in some mouse models of colitis.
33807914	3	97	theme	glycan	470:475	arg1	fucose					449:454	fucose	449:454	fucose	449:454	Previous work has shown that fucose, the terminal glycan chain component of the intestinal glycoprotein Mucin2, and fucoidan polysaccharides have an anti-inflammatory effect in some mouse models of colitis.
33807914	12	98	theme	pregnancy	2088:2096	arg1	outcome					2098:2104	pregnancy outcome	2088:2104	pregnancy outcome	2088:2104	Taken together, these data suggest that intestinal microflora affects host immunity and pregnancy outcome.
33807914	3	99	dep	fucose	449:454	arg1	polysaccharides					545:559	polysaccharides	545:559	polysaccharides	545:559	Previous work has shown that fucose, the terminal glycan chain component of the intestinal glycoprotein Mucin2, and fucoidan polysaccharides have an anti-inflammatory effect in some mouse models of colitis.
33807914	7	100	theme	implantation	1228:1239	arg1	impairment					1241:1250	implantation impairment	1228:1250	implantation impairment	1228:1250	We propose that the changes in beneficial microflora affect the immune status in Muc2 mice, which causes implantation impairment.
33807914	9	101	theme	peritoneal	1419:1428	arg1	macrophages					1430:1440	peritoneal macrophages	1419:1440	peritoneal macrophages from Muc2 females	1419:1458	Metabolic profiling of peritoneal macrophages from Muc2 females reveals their predisposition towards anaerobic glycolysis in favor of oxidative phosphorylation, compared to C57BL/6-derived cells.
33807914	10	102	from	experiments	1601:1611	arg1	activity					1629:1636	phagocytosis activity	1616:1636	phagocytosis activity	1616:1636	In vitro experiments on phagocytosis activity and mitochondrial respiration suggest that fucose affects oxidative phosphorylation in a genotype-specific manner, which might interfere with implantation depending on the initial status of macrophages.
33807914	10	102	from	experiments	1601:1611	arg1	respiration					1656:1666	mitochondrial respiration	1642:1666	mitochondrial respiration	1642:1666	In vitro experiments on phagocytosis activity and mitochondrial respiration suggest that fucose affects oxidative phosphorylation in a genotype-specific manner, which might interfere with implantation depending on the initial status of macrophages.
33807914	1	103	theme	epithelial	111:120	arg1	cells					133:137	epithelial and immune cells	111:137	epithelial and immune cells from the gut resident microorganisms	111:174	Intestinal mucus protects epithelial and immune cells from the gut resident microorganisms, and provides growth-promoting factors as mucus-derived O-glycans for beneficial bacteria.
33807914	10	104	theme	macrophages	1828:1838	arg1	status					1818:1823	the initial status	1806:1823	the initial status of macrophages	1806:1838	In vitro experiments on phagocytosis activity and mitochondrial respiration suggest that fucose affects oxidative phosphorylation in a genotype-specific manner, which might interfere with implantation depending on the initial status of macrophages.
33807914	10	105	theme	initial	1810:1816	arg1	status					1818:1823	the initial status	1806:1823	the initial status of macrophages	1806:1838	In vitro experiments on phagocytosis activity and mitochondrial respiration suggest that fucose affects oxidative phosphorylation in a genotype-specific manner, which might interfere with implantation depending on the initial status of macrophages.
33807914	13	106	theme	macrophage	2181:2190	arg1	polarization					2192:2203	macrophage polarization	2181:2203	macrophage polarization	2181:2203	At the same time, dietary fucose might act as a differential regulator of macrophage polarization during implantation, depending on the immune status of the host.
33807914	10	107	theme	mitochondrial	1642:1654	arg1	respiration					1656:1666	mitochondrial respiration	1642:1666	mitochondrial respiration	1642:1666	In vitro experiments on phagocytosis activity and mitochondrial respiration suggest that fucose affects oxidative phosphorylation in a genotype-specific manner, which might interfere with implantation depending on the initial status of macrophages.
33807914	11	108	theme	BALB/c	1881:1886	arg1	mice					1895:1898	BALB/c female mice	1881:1898	BALB/c female mice	1881:1898	This hypothesis is further confirmed in BALB/c female mice, where fucose caused pregnancy loss and opposed implantation-associated M2 macrophage polarization.
33807914	2	109	theme	mucosal	365:371	arg1	system					380:385	mucosal immune system	365:385	mucosal immune system reprogramming	365:399	A lack of intestinal protective mucus results in changes in the commensal microflora composition, mucosal immune system reprogramming, and inflammation.
33807914	6	110	contain	have	1021:1024	arg1	siblings					996:1003	the otherwise healthy wild-type siblings	964:1003	the otherwise healthy wild-type siblings of Muc2 animals	964:1019	Metagenomic analysis reveals that the otherwise healthy wild-type siblings of Muc2 animals have reduced numbers of some of the intestinal commensal bacterial species, compared to C57BL/6 mice.
33807914	6	110	contain	have	1021:1024	arg2	numbers					1034:1040	reduced numbers	1026:1040	reduced numbers of some of the intestinal commensal bacterial species	1026:1094	Metagenomic analysis reveals that the otherwise healthy wild-type siblings of Muc2 animals have reduced numbers of some of the intestinal commensal bacterial species, compared to C57BL/6 mice.
33807914	3	111	theme	glycoprotein	511:522	arg1	Mucin2					524:529	the intestinal glycoprotein Mucin2	496:529	the intestinal glycoprotein Mucin2	496:529	Previous work has shown that fucose, the terminal glycan chain component of the intestinal glycoprotein Mucin2, and fucoidan polysaccharides have an anti-inflammatory effect in some mouse models of colitis.
34684744	0	0	theme	Cryogel	90:96	arg1	Oxide					117:121	Calcium Cross-Linked Starch Cryogel Composite Graphene Oxide	62:121	Calcium Cross-Linked Starch Cryogel Composite Graphene Oxide Nanoparticles for Benzo(a)pyrene Analysis	62:163	Greener Monolithic Solid Phase Extraction Biosorbent Based on Calcium Cross-Linked Starch Cryogel Composite Graphene Oxide Nanoparticles for Benzo(a)pyrene Analysis.
34684744	2	1	from	nanoparticles	381:393	arg1	GO-Cry					435:440	GO-Cry	435:440	GO-Cry	435:440	In this work, a novel monolithic solid-phase extraction (SPE) sorbent based on graphene oxide nanoparticles (GO) in starch-based cryogel composite (GO-Cry) was successfully prepared for BaP analysis.
34684744	2	1	from	nanoparticles	381:393	arg1	composite					424:432	starch-based cryogel composite	403:432	starch-based cryogel composite (GO-Cry)	403:441	In this work, a novel monolithic solid-phase extraction (SPE) sorbent based on graphene oxide nanoparticles (GO) in starch-based cryogel composite (GO-Cry) was successfully prepared for BaP analysis.
34684744	2	2	theme	monolithic	309:318	arg1	sorbent					349:355	a novel monolithic solid-phase extraction (SPE) sorbent	301:355	a novel monolithic solid-phase extraction (SPE) sorbent based on graphene oxide nanoparticles (GO) in starch-based cryogel composite (GO-Cry)	301:441	In this work, a novel monolithic solid-phase extraction (SPE) sorbent based on graphene oxide nanoparticles (GO) in starch-based cryogel composite (GO-Cry) was successfully prepared for BaP analysis.
34684744	6	3	theme	significant	1073:1083	arg1	difference					1085:1094	no significant difference	1070:1094	no significant difference to a C18 cartridge	1070:1113	The accuracy in terms of recovery from spiked samples was in the range of 84 to 110% with no significant difference to a C18 cartridge.
34684744	5	4	dep	±	890:890	arg1	µgL-1					914:918	0.06 and 14.04 ± 0.19 µgL-1	892:918	0.06 and 14.04 ± 0.19 µgL-1	892:918	The limit of detection and limit of quantification were 4.21 ± 0.06 and 14.04 ± 0.19 µgL-1, respectively, with excellent precision (0.17 to 2.45%RSD).
34684744	0	5	theme	Starch	83:88	arg1	Cryogel					90:96	Calcium Cross-Linked Starch Cryogel	62:96	Calcium Cross-Linked Starch Cryogel Composite Graphene Oxide Nanoparticles for Benzo(a)pyrene Analysis	62:163	Greener Monolithic Solid Phase Extraction Biosorbent Based on Calcium Cross-Linked Starch Cryogel Composite Graphene Oxide Nanoparticles for Benzo(a)pyrene Analysis.
34684744	3	6	theme	GO	624:625	arg1	addition					612:619	addition	612:619	addition of GO (filler) that can increase the ability to extract BaP up to 2.6-fold	612:694	Rice flour and tapioca starch (gel precursors) were gelatinized in limewater (cross-linker) under alkaline conditions before addition of GO (filler) that can increase the ability to extract BaP up to 2.6-fold.
34684744	4	7	theme	good	754:757	arg1	linearity					759:767	good linearity	754:767	good linearity (R = 0.9971)	754:780	BaP analysis had a linear range of 10 to 1000 µgL-1 with good linearity (R = 0.9971) and high sensitivity (4.1 ± 0.1 a.u./(µgL-1)).
34684744	4	7	theme	good	754:757	arg1	R					770:770	R = 0.9971	770:779	R = 0.9971	770:779	BaP analysis had a linear range of 10 to 1000 µgL-1 with good linearity (R = 0.9971) and high sensitivity (4.1 ± 0.1 a.u./(µgL-1)).
34684744	2	8	theme	novel	303:307	arg1	sorbent					349:355	a novel monolithic solid-phase extraction (SPE) sorbent	301:355	a novel monolithic solid-phase extraction (SPE) sorbent based on graphene oxide nanoparticles (GO) in starch-based cryogel composite (GO-Cry)	301:441	In this work, a novel monolithic solid-phase extraction (SPE) sorbent based on graphene oxide nanoparticles (GO) in starch-based cryogel composite (GO-Cry) was successfully prepared for BaP analysis.
34684744	6	9	with	range	1045:1049	arg1	difference					1085:1094	no significant difference	1070:1094	no significant difference to a C18 cartridge	1070:1113	The accuracy in terms of recovery from spiked samples was in the range of 84 to 110% with no significant difference to a C18 cartridge.
34684744	0	10	theme	Graphene	108:115	arg1	Oxide					117:121	Calcium Cross-Linked Starch Cryogel Composite Graphene Oxide	62:121	Calcium Cross-Linked Starch Cryogel Composite Graphene Oxide Nanoparticles for Benzo(a)pyrene Analysis	62:163	Greener Monolithic Solid Phase Extraction Biosorbent Based on Calcium Cross-Linked Starch Cryogel Composite Graphene Oxide Nanoparticles for Benzo(a)pyrene Analysis.
34684744	3	11	theme	Rice	487:490	arg1	flour					492:496	Rice flour	487:496	Rice flour	487:496	Rice flour and tapioca starch (gel precursors) were gelatinized in limewater (cross-linker) under alkaline conditions before addition of GO (filler) that can increase the ability to extract BaP up to 2.6-fold.
34684744	4	12	dep	1000	738:741	arg1	to					735:736	to	735:736	to	735:736	BaP analysis had a linear range of 10 to 1000 µgL-1 with good linearity (R = 0.9971) and high sensitivity (4.1 ± 0.1 a.u./(µgL-1)).
34684744	0	13	theme	Composite	98:106	arg1	Oxide					117:121	Calcium Cross-Linked Starch Cryogel Composite Graphene Oxide	62:121	Calcium Cross-Linked Starch Cryogel Composite Graphene Oxide Nanoparticles for Benzo(a)pyrene Analysis	62:163	Greener Monolithic Solid Phase Extraction Biosorbent Based on Calcium Cross-Linked Starch Cryogel Composite Graphene Oxide Nanoparticles for Benzo(a)pyrene Analysis.
34684744	5	14	dep	2.45	969:972	arg1	to					966:967	to	966:967	to	966:967	The limit of detection and limit of quantification were 4.21 ± 0.06 and 14.04 ± 0.19 µgL-1, respectively, with excellent precision (0.17 to 2.45%RSD).
34684744	6	15	theme	C18	1101:1103	arg1	cartridge					1105:1113	a C18 cartridge	1099:1113	a C18 cartridge	1099:1113	The accuracy in terms of recovery from spiked samples was in the range of 84 to 110% with no significant difference to a C18 cartridge.
34684744	4	16	dep	sensitivity	791:801	arg1	a.u./					814:818	4.1 ± 0.1 a.u./	804:818	4.1 ± 0.1 a.u./(µgL-1)	804:825	BaP analysis had a linear range of 10 to 1000 µgL-1 with good linearity (R = 0.9971) and high sensitivity (4.1 ± 0.1 a.u./(µgL-1)).
34684744	4	16	dep	sensitivity	791:801	arg1	µgL-1					820:824	µgL-1	820:824	µgL-1	820:824	BaP analysis had a linear range of 10 to 1000 µgL-1 with good linearity (R = 0.9971) and high sensitivity (4.1 ± 0.1 a.u./(µgL-1)).
34684744	5	17	dep	%	973:973	arg1	2.45					969:972	2.45	969:972	2.45	969:972	The limit of detection and limit of quantification were 4.21 ± 0.06 and 14.04 ± 0.19 µgL-1, respectively, with excellent precision (0.17 to 2.45%RSD).
34684744	6	18	from	accuracy	984:991	arg1	terms					996:1000	terms	996:1000	terms of recovery from spiked samples	996:1032	The accuracy in terms of recovery from spiked samples was in the range of 84 to 110% with no significant difference to a C18 cartridge.
34684744	2	19	theme	SPE	344:346	arg1	sorbent					349:355	a novel monolithic solid-phase extraction (SPE) sorbent	301:355	a novel monolithic solid-phase extraction (SPE) sorbent based on graphene oxide nanoparticles (GO) in starch-based cryogel composite (GO-Cry)	301:441	In this work, a novel monolithic solid-phase extraction (SPE) sorbent based on graphene oxide nanoparticles (GO) in starch-based cryogel composite (GO-Cry) was successfully prepared for BaP analysis.
34684744	1	20	theme	Benzo	166:170	arg1	marker					212:217	a marker	210:217	a marker for the detection of carcinogenic polycyclic aromatic hydrocarbons	210:284	Benzo(a)pyrene (BaP) has been recognized as a marker for the detection of carcinogenic polycyclic aromatic hydrocarbons.
34684744	1	20	theme	Benzo	166:170	arg1	BaP					182:184	BaP	182:184	BaP	182:184	Benzo(a)pyrene (BaP) has been recognized as a marker for the detection of carcinogenic polycyclic aromatic hydrocarbons.
34684744	1	20	theme	Benzo	166:170	arg1	pyrene					174:179	Benzo(a)pyrene	166:179	Benzo(a)pyrene (BaP)	166:185	Benzo(a)pyrene (BaP) has been recognized as a marker for the detection of carcinogenic polycyclic aromatic hydrocarbons.
34684744	0	21	theme	Oxide	117:121	arg1	Nanoparticles					123:135	Calcium Cross-Linked Starch Cryogel Composite Graphene Oxide Nanoparticles	62:135	Calcium Cross-Linked Starch Cryogel Composite Graphene Oxide Nanoparticles for Benzo(a)pyrene Analysis	62:163	Greener Monolithic Solid Phase Extraction Biosorbent Based on Calcium Cross-Linked Starch Cryogel Composite Graphene Oxide Nanoparticles for Benzo(a)pyrene Analysis.
34684744	2	22	dep	nanoparticles	381:393	arg1	GO					396:397	GO	396:397	GO	396:397	In this work, a novel monolithic solid-phase extraction (SPE) sorbent based on graphene oxide nanoparticles (GO) in starch-based cryogel composite (GO-Cry) was successfully prepared for BaP analysis.
34684744	5	23	theme	excellent	940:948	arg1	precision					950:958	excellent precision	940:958	excellent precision (0.17 to 2.45%RSD)	940:977	The limit of detection and limit of quantification were 4.21 ± 0.06 and 14.04 ± 0.19 µgL-1, respectively, with excellent precision (0.17 to 2.45%RSD).
34684744	5	23	theme	excellent	940:948	arg1	RSD					974:976	0.17 to 2.45%RSD	961:976	0.17 to 2.45%RSD	961:976	The limit of detection and limit of quantification were 4.21 ± 0.06 and 14.04 ± 0.19 µgL-1, respectively, with excellent precision (0.17 to 2.45%RSD).
34684744	6	24	dep	110	1060:1062	arg1	to					1057:1058	to	1057:1058	to	1057:1058	The accuracy in terms of recovery from spiked samples was in the range of 84 to 110% with no significant difference to a C18 cartridge.
34684744	0	25	theme	Monolithic	8:17	arg1	Extraction					31:40	Greener Monolithic Solid Phase Extraction	0:40	Greener Monolithic Solid Phase Extraction Biosorbent	0:51	Greener Monolithic Solid Phase Extraction Biosorbent Based on Calcium Cross-Linked Starch Cryogel Composite Graphene Oxide Nanoparticles for Benzo(a)pyrene Analysis.
34684744	6	26	theme	spiked	1019:1024	arg1	samples					1026:1032	spiked samples	1019:1032	spiked samples	1019:1032	The accuracy in terms of recovery from spiked samples was in the range of 84 to 110% with no significant difference to a C18 cartridge.
34684744	5	27	with	4.21	885:888	arg1	precision					950:958	excellent precision	940:958	excellent precision (0.17 to 2.45%RSD)	940:977	The limit of detection and limit of quantification were 4.21 ± 0.06 and 14.04 ± 0.19 µgL-1, respectively, with excellent precision (0.17 to 2.45%RSD).
34684744	5	27	with	4.21	885:888	arg1	RSD					974:976	0.17 to 2.45%RSD	961:976	0.17 to 2.45%RSD	961:976	The limit of detection and limit of quantification were 4.21 ± 0.06 and 14.04 ± 0.19 µgL-1, respectively, with excellent precision (0.17 to 2.45%RSD).
34684744	1	28	theme	a	172:172	arg1	marker					212:217	a marker	210:217	a marker for the detection of carcinogenic polycyclic aromatic hydrocarbons	210:284	Benzo(a)pyrene (BaP) has been recognized as a marker for the detection of carcinogenic polycyclic aromatic hydrocarbons.
34684744	1	28	theme	a	172:172	arg1	BaP					182:184	BaP	182:184	BaP	182:184	Benzo(a)pyrene (BaP) has been recognized as a marker for the detection of carcinogenic polycyclic aromatic hydrocarbons.
34684744	1	28	theme	a	172:172	arg1	pyrene					174:179	Benzo(a)pyrene	166:179	Benzo(a)pyrene (BaP)	166:185	Benzo(a)pyrene (BaP) has been recognized as a marker for the detection of carcinogenic polycyclic aromatic hydrocarbons.
34684744	0	29	theme	Greener	0:6	arg1	Extraction					31:40	Greener Monolithic Solid Phase Extraction	0:40	Greener Monolithic Solid Phase Extraction Biosorbent	0:51	Greener Monolithic Solid Phase Extraction Biosorbent Based on Calcium Cross-Linked Starch Cryogel Composite Graphene Oxide Nanoparticles for Benzo(a)pyrene Analysis.
34684744	3	30	theme	extract	669:675	arg1	BaP					677:679	extract BaP	669:679	extract BaP	669:679	Rice flour and tapioca starch (gel precursors) were gelatinized in limewater (cross-linker) under alkaline conditions before addition of GO (filler) that can increase the ability to extract BaP up to 2.6-fold.
34684744	4	31	theme	µgL-1	743:747	arg1	range					723:727	a linear range	714:727	a linear range of 10 to 1000 µgL-1	714:747	BaP analysis had a linear range of 10 to 1000 µgL-1 with good linearity (R = 0.9971) and high sensitivity (4.1 ± 0.1 a.u./(µgL-1)).
34684744	0	32	theme	Phase	25:29	arg1	Extraction					31:40	Greener Monolithic Solid Phase Extraction	0:40	Greener Monolithic Solid Phase Extraction Biosorbent	0:51	Greener Monolithic Solid Phase Extraction Biosorbent Based on Calcium Cross-Linked Starch Cryogel Composite Graphene Oxide Nanoparticles for Benzo(a)pyrene Analysis.
34684744	0	33	theme	Benzo	141:145	arg1	pyrene					149:154	Benzo(a)pyrene	141:154	Benzo(a)pyrene Analysis	141:163	Greener Monolithic Solid Phase Extraction Biosorbent Based on Calcium Cross-Linked Starch Cryogel Composite Graphene Oxide Nanoparticles for Benzo(a)pyrene Analysis.
34684744	6	34	theme	recovery	1005:1012	arg1	terms					996:1000	terms	996:1000	terms of recovery from spiked samples	996:1032	The accuracy in terms of recovery from spiked samples was in the range of 84 to 110% with no significant difference to a C18 cartridge.
34684744	7	35	theme	2.8	1157:1159	arg1	%					1160:1160	%	1160:1160	%	1160:1160	GO-Cry can be reproducibly prepared with 2.8%RSD from 4 lots and can be reused at least 10 times, which not only helps reduce the analysis costs (~0.41USD per analysis), but also reduces the resultant waste to the environment.
34684744	2	36	theme	BaP	473:475	arg1	analysis					477:484	BaP analysis	473:484	BaP analysis	473:484	In this work, a novel monolithic solid-phase extraction (SPE) sorbent based on graphene oxide nanoparticles (GO) in starch-based cryogel composite (GO-Cry) was successfully prepared for BaP analysis.
34684744	0	37	theme	Solid	19:23	arg1	Extraction					31:40	Greener Monolithic Solid Phase Extraction	0:40	Greener Monolithic Solid Phase Extraction Biosorbent	0:51	Greener Monolithic Solid Phase Extraction Biosorbent Based on Calcium Cross-Linked Starch Cryogel Composite Graphene Oxide Nanoparticles for Benzo(a)pyrene Analysis.
34684744	1	38	theme	carcinogenic	240:251	arg1	hydrocarbons					273:284	carcinogenic polycyclic aromatic hydrocarbons	240:284	carcinogenic polycyclic aromatic hydrocarbons	240:284	Benzo(a)pyrene (BaP) has been recognized as a marker for the detection of carcinogenic polycyclic aromatic hydrocarbons.
34684744	4	39	theme	high	786:789	arg1	sensitivity					791:801	high sensitivity	786:801	high sensitivity (4.1 ± 0.1 a.u./(µgL-1))	786:826	BaP analysis had a linear range of 10 to 1000 µgL-1 with good linearity (R = 0.9971) and high sensitivity (4.1 ± 0.1 a.u./(µgL-1)).
34684744	3	40	theme	tapioca	502:508	arg1	starch					510:515	tapioca starch	502:515	tapioca starch (gel precursors)	502:532	Rice flour and tapioca starch (gel precursors) were gelatinized in limewater (cross-linker) under alkaline conditions before addition of GO (filler) that can increase the ability to extract BaP up to 2.6-fold.
34684744	3	40	theme	tapioca	502:508	arg1	precursors					522:531	gel precursors	518:531	gel precursors	518:531	Rice flour and tapioca starch (gel precursors) were gelatinized in limewater (cross-linker) under alkaline conditions before addition of GO (filler) that can increase the ability to extract BaP up to 2.6-fold.
34684744	2	41	theme	graphene	366:373	arg1	nanoparticles					381:393	graphene oxide nanoparticles	366:393	graphene oxide nanoparticles (GO) in starch-based cryogel composite (GO-Cry)	366:441	In this work, a novel monolithic solid-phase extraction (SPE) sorbent based on graphene oxide nanoparticles (GO) in starch-based cryogel composite (GO-Cry) was successfully prepared for BaP analysis.
34684744	1	42	theme	polycyclic	253:262	arg1	hydrocarbons					273:284	carcinogenic polycyclic aromatic hydrocarbons	240:284	carcinogenic polycyclic aromatic hydrocarbons	240:284	Benzo(a)pyrene (BaP) has been recognized as a marker for the detection of carcinogenic polycyclic aromatic hydrocarbons.
34684744	0	43	theme	a	147:147	arg1	pyrene					149:154	Benzo(a)pyrene	141:154	Benzo(a)pyrene Analysis	141:163	Greener Monolithic Solid Phase Extraction Biosorbent Based on Calcium Cross-Linked Starch Cryogel Composite Graphene Oxide Nanoparticles for Benzo(a)pyrene Analysis.
34684744	3	44	theme	alkaline	585:592	arg1	conditions					594:603	alkaline conditions	585:603	alkaline conditions	585:603	Rice flour and tapioca starch (gel precursors) were gelatinized in limewater (cross-linker) under alkaline conditions before addition of GO (filler) that can increase the ability to extract BaP up to 2.6-fold.
34684744	0	45	theme	Extraction	31:40	arg1	Biosorbent					42:51	Greener Monolithic Solid Phase Extraction Biosorbent	0:51	Greener Monolithic Solid Phase Extraction Biosorbent	0:51	Greener Monolithic Solid Phase Extraction Biosorbent Based on Calcium Cross-Linked Starch Cryogel Composite Graphene Oxide Nanoparticles for Benzo(a)pyrene Analysis.
34684744	1	46	theme	aromatic	264:271	arg1	hydrocarbons					273:284	carcinogenic polycyclic aromatic hydrocarbons	240:284	carcinogenic polycyclic aromatic hydrocarbons	240:284	Benzo(a)pyrene (BaP) has been recognized as a marker for the detection of carcinogenic polycyclic aromatic hydrocarbons.
34684744	2	47	theme	cryogel	416:422	arg1	GO-Cry					435:440	GO-Cry	435:440	GO-Cry	435:440	In this work, a novel monolithic solid-phase extraction (SPE) sorbent based on graphene oxide nanoparticles (GO) in starch-based cryogel composite (GO-Cry) was successfully prepared for BaP analysis.
34684744	2	47	theme	cryogel	416:422	arg1	composite					424:432	starch-based cryogel composite	403:432	starch-based cryogel composite (GO-Cry)	403:441	In this work, a novel monolithic solid-phase extraction (SPE) sorbent based on graphene oxide nanoparticles (GO) in starch-based cryogel composite (GO-Cry) was successfully prepared for BaP analysis.
34684744	4	48	theme	linear	716:721	arg1	range					723:727	a linear range	714:727	a linear range of 10 to 1000 µgL-1	714:747	BaP analysis had a linear range of 10 to 1000 µgL-1 with good linearity (R = 0.9971) and high sensitivity (4.1 ± 0.1 a.u./(µgL-1)).
34684744	2	49	theme	starch-based	403:414	arg1	GO-Cry					435:440	GO-Cry	435:440	GO-Cry	435:440	In this work, a novel monolithic solid-phase extraction (SPE) sorbent based on graphene oxide nanoparticles (GO) in starch-based cryogel composite (GO-Cry) was successfully prepared for BaP analysis.
34684744	2	49	theme	starch-based	403:414	arg1	composite					424:432	starch-based cryogel composite	403:432	starch-based cryogel composite (GO-Cry)	403:441	In this work, a novel monolithic solid-phase extraction (SPE) sorbent based on graphene oxide nanoparticles (GO) in starch-based cryogel composite (GO-Cry) was successfully prepared for BaP analysis.
34684744	1	50	theme	hydrocarbons	273:284	arg1	detection					227:235	the detection	223:235	the detection of carcinogenic polycyclic aromatic hydrocarbons	223:284	Benzo(a)pyrene (BaP) has been recognized as a marker for the detection of carcinogenic polycyclic aromatic hydrocarbons.
34684744	0	51	theme	pyrene	149:154	arg1	Analysis					156:163	Benzo(a)pyrene Analysis	141:163	Benzo(a)pyrene Analysis	141:163	Greener Monolithic Solid Phase Extraction Biosorbent Based on Calcium Cross-Linked Starch Cryogel Composite Graphene Oxide Nanoparticles for Benzo(a)pyrene Analysis.
34684744	7	52	theme	analysis	1246:1253	arg1	costs					1255:1259	the analysis costs	1242:1259	the analysis costs (~0.41USD per analysis)	1242:1283	GO-Cry can be reproducibly prepared with 2.8%RSD from 4 lots and can be reused at least 10 times, which not only helps reduce the analysis costs (~0.41USD per analysis), but also reduces the resultant waste to the environment.
34684744	3	53	theme	gel	518:520	arg1	starch					510:515	tapioca starch	502:515	tapioca starch (gel precursors)	502:532	Rice flour and tapioca starch (gel precursors) were gelatinized in limewater (cross-linker) under alkaline conditions before addition of GO (filler) that can increase the ability to extract BaP up to 2.6-fold.
34684744	3	53	theme	gel	518:520	arg1	precursors					522:531	gel precursors	518:531	gel precursors	518:531	Rice flour and tapioca starch (gel precursors) were gelatinized in limewater (cross-linker) under alkaline conditions before addition of GO (filler) that can increase the ability to extract BaP up to 2.6-fold.
34684744	5	54	dep	RSD	974:976	arg1	%					973:973	%	973:973	%	973:973	The limit of detection and limit of quantification were 4.21 ± 0.06 and 14.04 ± 0.19 µgL-1, respectively, with excellent precision (0.17 to 2.45%RSD).
34684744	4	55	theme	=	772:772	arg1	linearity					759:767	good linearity	754:767	good linearity (R = 0.9971)	754:780	BaP analysis had a linear range of 10 to 1000 µgL-1 with good linearity (R = 0.9971) and high sensitivity (4.1 ± 0.1 a.u./(µgL-1)).
34684744	4	55	theme	=	772:772	arg1	R					770:770	R = 0.9971	770:779	R = 0.9971	770:779	BaP analysis had a linear range of 10 to 1000 µgL-1 with good linearity (R = 0.9971) and high sensitivity (4.1 ± 0.1 a.u./(µgL-1)).
34684744	5	56	theme	quantification	865:878	arg1	limit					856:860	detection and limit	842:860	limit	856:860	The limit of detection and limit of quantification were 4.21 ± 0.06 and 14.04 ± 0.19 µgL-1, respectively, with excellent precision (0.17 to 2.45%RSD).
34684744	5	56	theme	quantification	865:878	arg1	detection					842:850	detection and limit	842:860	detection	842:850	The limit of detection and limit of quantification were 4.21 ± 0.06 and 14.04 ± 0.19 µgL-1, respectively, with excellent precision (0.17 to 2.45%RSD).
34684744	7	57	theme	resultant	1307:1315	arg1	waste					1317:1321	the resultant waste	1303:1321	the resultant waste to the environment	1303:1340	GO-Cry can be reproducibly prepared with 2.8%RSD from 4 lots and can be reused at least 10 times, which not only helps reduce the analysis costs (~0.41USD per analysis), but also reduces the resultant waste to the environment.
34684744	5	58	theme	limit	856:860	arg1	limit					833:837	The limit	829:837	The limit of detection and limit of quantification	829:878	The limit of detection and limit of quantification were 4.21 ± 0.06 and 14.04 ± 0.19 µgL-1, respectively, with excellent precision (0.17 to 2.45%RSD).
34684744	5	58	theme	limit	856:860	arg1	4.21					885:888	4.21	885:888	4.21 ± 0.06 and 14.04 ± 0.19 µgL-1	885:918	The limit of detection and limit of quantification were 4.21 ± 0.06 and 14.04 ± 0.19 µgL-1, respectively, with excellent precision (0.17 to 2.45%RSD).
34684744	4	59	contain	had	710:712	arg2	range					723:727	a linear range	714:727	a linear range of 10 to 1000 µgL-1	714:747	BaP analysis had a linear range of 10 to 1000 µgL-1 with good linearity (R = 0.9971) and high sensitivity (4.1 ± 0.1 a.u./(µgL-1)).
34684744	4	59	contain	had	710:712	arg1	analysis					701:708	BaP analysis	697:708	BaP analysis	697:708	BaP analysis had a linear range of 10 to 1000 µgL-1 with good linearity (R = 0.9971) and high sensitivity (4.1 ± 0.1 a.u./(µgL-1)).
34684744	6	60	theme	%	1063:1063	arg1	range					1045:1049	the range	1041:1049	the range of 84 to 110% with no significant difference to a C18 cartridge	1041:1113	The accuracy in terms of recovery from spiked samples was in the range of 84 to 110% with no significant difference to a C18 cartridge.
34684744	5	61	theme	±	890:890	arg1	4.21					885:888	4.21	885:888	4.21 ± 0.06 and 14.04 ± 0.19 µgL-1	885:918	The limit of detection and limit of quantification were 4.21 ± 0.06 and 14.04 ± 0.19 µgL-1, respectively, with excellent precision (0.17 to 2.45%RSD).
34684744	5	61	theme	±	890:890	arg1	limit					833:837	The limit	829:837	The limit of detection and limit of quantification	829:878	The limit of detection and limit of quantification were 4.21 ± 0.06 and 14.04 ± 0.19 µgL-1, respectively, with excellent precision (0.17 to 2.45%RSD).
34684744	4	62	theme	BaP	697:699	arg1	analysis					701:708	BaP analysis	697:708	BaP analysis	697:708	BaP analysis had a linear range of 10 to 1000 µgL-1 with good linearity (R = 0.9971) and high sensitivity (4.1 ± 0.1 a.u./(µgL-1)).
34684744	6	63	from	samples	1026:1032	arg1	terms					996:1000	terms	996:1000	terms of recovery from spiked samples	996:1032	The accuracy in terms of recovery from spiked samples was in the range of 84 to 110% with no significant difference to a C18 cartridge.
34684744	6	63	from	samples	1026:1032	arg1	recovery					1005:1012	recovery	1005:1012	recovery from spiked samples	1005:1032	The accuracy in terms of recovery from spiked samples was in the range of 84 to 110% with no significant difference to a C18 cartridge.
34684744	0	64	theme	Cross-Linked	70:81	arg1	Cryogel					90:96	Calcium Cross-Linked Starch Cryogel	62:96	Calcium Cross-Linked Starch Cryogel Composite Graphene Oxide Nanoparticles for Benzo(a)pyrene Analysis	62:163	Greener Monolithic Solid Phase Extraction Biosorbent Based on Calcium Cross-Linked Starch Cryogel Composite Graphene Oxide Nanoparticles for Benzo(a)pyrene Analysis.
34684744	6	65	from	terms	996:1000	arg1	samples					1026:1032	spiked samples	1019:1032	spiked samples	1019:1032	The accuracy in terms of recovery from spiked samples was in the range of 84 to 110% with no significant difference to a C18 cartridge.
34684744	2	66	theme	oxide	375:379	arg1	nanoparticles					381:393	graphene oxide nanoparticles	366:393	graphene oxide nanoparticles (GO) in starch-based cryogel composite (GO-Cry)	366:441	In this work, a novel monolithic solid-phase extraction (SPE) sorbent based on graphene oxide nanoparticles (GO) in starch-based cryogel composite (GO-Cry) was successfully prepared for BaP analysis.
34684744	2	67	theme	extraction	332:341	arg1	sorbent					349:355	a novel monolithic solid-phase extraction (SPE) sorbent	301:355	a novel monolithic solid-phase extraction (SPE) sorbent based on graphene oxide nanoparticles (GO) in starch-based cryogel composite (GO-Cry)	301:441	In this work, a novel monolithic solid-phase extraction (SPE) sorbent based on graphene oxide nanoparticles (GO) in starch-based cryogel composite (GO-Cry) was successfully prepared for BaP analysis.
34684744	7	68	dep	costs	1255:1259	arg1	~0.41USD					1262:1269	~0.41USD	1262:1269	~0.41USD	1262:1269	GO-Cry can be reproducibly prepared with 2.8%RSD from 4 lots and can be reused at least 10 times, which not only helps reduce the analysis costs (~0.41USD per analysis), but also reduces the resultant waste to the environment.
34684744	0	69	theme	Calcium	62:68	arg1	Cryogel					90:96	Calcium Cross-Linked Starch Cryogel	62:96	Calcium Cross-Linked Starch Cryogel Composite Graphene Oxide Nanoparticles for Benzo(a)pyrene Analysis	62:163	Greener Monolithic Solid Phase Extraction Biosorbent Based on Calcium Cross-Linked Starch Cryogel Composite Graphene Oxide Nanoparticles for Benzo(a)pyrene Analysis.
34684744	5	70	theme	detection	842:850	arg1	limit					833:837	The limit	829:837	The limit of detection and limit of quantification	829:878	The limit of detection and limit of quantification were 4.21 ± 0.06 and 14.04 ± 0.19 µgL-1, respectively, with excellent precision (0.17 to 2.45%RSD).
34684744	5	70	theme	detection	842:850	arg1	4.21					885:888	4.21	885:888	4.21 ± 0.06 and 14.04 ± 0.19 µgL-1	885:918	The limit of detection and limit of quantification were 4.21 ± 0.06 and 14.04 ± 0.19 µgL-1, respectively, with excellent precision (0.17 to 2.45%RSD).
34684744	2	71	theme	solid-phase	320:330	arg1	sorbent					349:355	a novel monolithic solid-phase extraction (SPE) sorbent	301:355	a novel monolithic solid-phase extraction (SPE) sorbent based on graphene oxide nanoparticles (GO) in starch-based cryogel composite (GO-Cry)	301:441	In this work, a novel monolithic solid-phase extraction (SPE) sorbent based on graphene oxide nanoparticles (GO) in starch-based cryogel composite (GO-Cry) was successfully prepared for BaP analysis.
34684744	7	72	theme	%	1160:1160	arg1	RSD					1161:1163	2.8%RSD	1157:1163	2.8%RSD	1157:1163	GO-Cry can be reproducibly prepared with 2.8%RSD from 4 lots and can be reused at least 10 times, which not only helps reduce the analysis costs (~0.41USD per analysis), but also reduces the resultant waste to the environment.
34684744	4	73	theme	±	808:808	arg1	a.u./					814:818	4.1 ± 0.1 a.u./	804:818	4.1 ± 0.1 a.u./(µgL-1)	804:825	BaP analysis had a linear range of 10 to 1000 µgL-1 with good linearity (R = 0.9971) and high sensitivity (4.1 ± 0.1 a.u./(µgL-1)).
34684744	4	73	theme	±	808:808	arg1	µgL-1					820:824	µgL-1	820:824	µgL-1	820:824	BaP analysis had a linear range of 10 to 1000 µgL-1 with good linearity (R = 0.9971) and high sensitivity (4.1 ± 0.1 a.u./(µgL-1)).
34558859	6	0	theme	mass	834:837	arg1	spectrometry					839:850	liquid chromatography-tandem mass spectrometry	805:850	liquid chromatography-tandem mass spectrometry	805:850	Gut microbiomes were analyzed by fecal 16S ribosomal RNA gene sequencing, and BA composition was determined by liquid chromatography-tandem mass spectrometry.
34558859	4	1	theme	BA	511:512	arg1	composition					514:524	BA composition	511:524	BA composition	511:524	Then, we studied the effects of the changed gut microbiota on BA composition and liver function.
34558859	11	2	theme	chronic	1515:1521	arg1	injury					1529:1534	chronic liver injury	1515:1534	chronic liver injury observed in DKO mice	1515:1555	In addition, the HFHSD-induced change of gut microbiota inhibited BA deconjugation and 7α-dehydroxylation in both types of mice, which improved chronic liver injury observed in DKO mice.
34558859	5	3	theme	human-like	570:579	arg1	Cyp2a12/Cyp2c70					581:595	human-like Cyp2a12/Cyp2c70	570:595	human-like Cyp2a12/Cyp2c70	570:595	Male wild-type (WT) and human-like Cyp2a12/Cyp2c70 double knockout (DKO) mice derived from C57BL/6J were fed with normal chow or HFHSD for 4 weeks.
34558859	6	4	theme	fecal	727:731	arg1	sequencing					756:765	fecal 16S ribosomal RNA gene sequencing	727:765	fecal 16S ribosomal RNA gene sequencing	727:765	Gut microbiomes were analyzed by fecal 16S ribosomal RNA gene sequencing, and BA composition was determined by liquid chromatography-tandem mass spectrometry.
34558859	8	5	theme	gut	1091:1093	arg1	microbiota					1095:1104	gut microbiota	1091:1104	gut microbiota	1091:1104	Despite the marked difference in the fecal BA composition, the profiles of gut microbiota in the two mouse models were quite similar.
34558859	6	6	theme	liquid	805:810	arg1	spectrometry					839:850	liquid chromatography-tandem mass spectrometry	805:850	liquid chromatography-tandem mass spectrometry	805:850	Gut microbiomes were analyzed by fecal 16S ribosomal RNA gene sequencing, and BA composition was determined by liquid chromatography-tandem mass spectrometry.
34558859	5	7	theme	double	597:602	arg1	mice					619:622	Male wild-type (WT) and human-like Cyp2a12/Cyp2c70 double knockout (DKO) mice	546:622	Male wild-type (WT) and human-like Cyp2a12/Cyp2c70 double knockout (DKO) mice derived from C57BL/6J	546:644	Male wild-type (WT) and human-like Cyp2a12/Cyp2c70 double knockout (DKO) mice derived from C57BL/6J were fed with normal chow or HFHSD for 4 weeks.
34558859	13	8	theme	liver	1816:1820	arg1	injury					1822:1827	liver injury	1816:1827	liver injury in the mouse model with human-like hydrophobic BA composition	1816:1889	On the contrary, the gut microbiota formed by HFHSD affects BA composition and ameliorates liver injury in the mouse model with human-like hydrophobic BA composition.
34558859	10	9	theme	mouse	1306:1310	arg1	models					1312:1317	the two mouse models	1298:1317	the two mouse models	1298:1317	However, microbial composition in the two mouse models was drastically but similarly changed by the HFHSD.
34558859	9	10	from	excretion	1222:1230	arg1	pool					1204:1207	the BA pool	1197:1207	the BA pool	1197:1207	An HFHSD resulted in a significant increase in the BA pool and fecal BA excretion in WT mice but not in DKO mice.
34558859	9	10	from	excretion	1222:1230	arg1	mice					1238:1241	WT mice	1235:1241	WT mice	1235:1241	An HFHSD resulted in a significant increase in the BA pool and fecal BA excretion in WT mice but not in DKO mice.
34558859	11	11	theme	BA	1437:1438	arg1	deconjugation					1440:1452	BA deconjugation	1437:1452	BA deconjugation	1437:1452	In addition, the HFHSD-induced change of gut microbiota inhibited BA deconjugation and 7α-dehydroxylation in both types of mice, which improved chronic liver injury observed in DKO mice.
34558859	12	12	theme	microbiota	1612:1621	arg1	change					1598:1603	the change	1594:1603	the change of gut microbiota due to HFHSD	1594:1634	Conclusion: The HFHSD itself causes the change of gut microbiota due to HFHSD, and the altered composition or concentration of BAs by HFHSD is not the primary factor.
34558859	11	13	theme	microbiota	1416:1425	arg1	change					1402:1407	the HFHSD-induced change	1384:1407	the HFHSD-induced change of gut microbiota	1384:1425	In addition, the HFHSD-induced change of gut microbiota inhibited BA deconjugation and 7α-dehydroxylation in both types of mice, which improved chronic liver injury observed in DKO mice.
34558859	0	14	theme	Acid	103:106	arg1	Composition					108:118	Human-Like Bile Acid Composition	87:118	Human-Like Bile Acid Composition	87:118	Western Diet Changes Gut Microbiota and Ameliorates Liver Injury in a Mouse Model with Human-Like Bile Acid Composition.
34558859	1	15	theme	bile	197:200	arg1	BA					208:209	BA	208:209	BA	208:209	Western-style high-fat/high-sucrose diet (HFHSD) changes gut microbiota and bile acid (BA) profiles.
34558859	1	15	theme	bile	197:200	arg1	acid					202:205	bile acid	197:205	bile acid (BA)	197:210	Western-style high-fat/high-sucrose diet (HFHSD) changes gut microbiota and bile acid (BA) profiles.
34558859	7	16	theme	lithocholic	997:1007	arg1	acids					1009:1013	chenodeoxycholic and lithocholic acids	976:1013	chenodeoxycholic and lithocholic acids	976:1013	The DKO mice exhibited significantly reduced fecal BA concentration, lacked muricholic acids, and increased proportions of chenodeoxycholic and lithocholic acids.
34558859	0	17	from	Injury	58:63	arg1	Model					76:80	a Mouse Model	68:80	a Mouse Model with Human-Like Bile Acid Composition	68:118	Western Diet Changes Gut Microbiota and Ameliorates Liver Injury in a Mouse Model with Human-Like Bile Acid Composition.
34558859	7	18	theme	chenodeoxycholic	976:991	arg1	acids					1009:1013	chenodeoxycholic and lithocholic acids	976:1013	chenodeoxycholic and lithocholic acids	976:1013	The DKO mice exhibited significantly reduced fecal BA concentration, lacked muricholic acids, and increased proportions of chenodeoxycholic and lithocholic acids.
34558859	7	19	theme	muricholic	929:938	arg1	acids					940:944	muricholic acids	929:944	muricholic acids	929:944	The DKO mice exhibited significantly reduced fecal BA concentration, lacked muricholic acids, and increased proportions of chenodeoxycholic and lithocholic acids.
34558859	1	20	theme	Western-style	121:133	arg1	diet					157:160	Western-style high-fat/high-sucrose diet	121:160	Western-style high-fat/high-sucrose diet (HFHSD)	121:168	Western-style high-fat/high-sucrose diet (HFHSD) changes gut microbiota and bile acid (BA) profiles.
34558859	1	20	theme	Western-style	121:133	arg1	HFHSD					163:167	HFHSD	163:167	HFHSD	163:167	Western-style high-fat/high-sucrose diet (HFHSD) changes gut microbiota and bile acid (BA) profiles.
34558859	7	21	theme	BA	904:905	arg1	concentration					907:919	significantly reduced fecal BA concentration	876:919	significantly reduced fecal BA concentration	876:919	The DKO mice exhibited significantly reduced fecal BA concentration, lacked muricholic acids, and increased proportions of chenodeoxycholic and lithocholic acids.
34558859	13	22	from	injury	1822:1827	arg1	model					1842:1846	the mouse model	1832:1846	the mouse model with human-like hydrophobic BA composition	1832:1889	On the contrary, the gut microbiota formed by HFHSD affects BA composition and ameliorates liver injury in the mouse model with human-like hydrophobic BA composition.
34558859	7	23	theme	reduced	890:896	arg1	concentration					907:919	significantly reduced fecal BA concentration	876:919	significantly reduced fecal BA concentration	876:919	The DKO mice exhibited significantly reduced fecal BA concentration, lacked muricholic acids, and increased proportions of chenodeoxycholic and lithocholic acids.
34558859	2	24	from	mechanism	285:293	arg1	both					309:312	both	309:312	both	309:312	Because gut microbiota and BAs could influence each other, the mechanism of changes in both by HFHSD is complicated and remains unclear.
34558859	6	25	theme	BA	772:773	arg1	composition					775:785	BA composition	772:785	BA composition	772:785	Gut microbiomes were analyzed by fecal 16S ribosomal RNA gene sequencing, and BA composition was determined by liquid chromatography-tandem mass spectrometry.
34558859	9	26	theme	fecal	1213:1217	arg1	excretion					1222:1230	fecal BA excretion	1213:1230	fecal BA excretion	1213:1230	An HFHSD resulted in a significant increase in the BA pool and fecal BA excretion in WT mice but not in DKO mice.
34558859	12	27	dep	composition	1653:1663	arg1	the					1641:1643	the	1641:1643	the	1641:1643	Conclusion: The HFHSD itself causes the change of gut microbiota due to HFHSD, and the altered composition or concentration of BAs by HFHSD is not the primary factor.
34558859	0	28	theme	Human-Like	87:96	arg1	Composition					108:118	Human-Like Bile Acid Composition	87:118	Human-Like Bile Acid Composition	87:118	Western Diet Changes Gut Microbiota and Ameliorates Liver Injury in a Mouse Model with Human-Like Bile Acid Composition.
34558859	7	29	theme	DKO	857:859	arg1	mice					861:864	The DKO mice	853:864	The DKO mice	853:864	The DKO mice exhibited significantly reduced fecal BA concentration, lacked muricholic acids, and increased proportions of chenodeoxycholic and lithocholic acids.
34558859	0	30	theme	Western	0:6	arg1	Diet					8:11	Western Diet	0:11	Western Diet	0:11	Western Diet Changes Gut Microbiota and Ameliorates Liver Injury in a Mouse Model with Human-Like Bile Acid Composition.
34558859	9	31	theme	WT	1235:1236	arg1	mice					1238:1241	WT mice	1235:1241	WT mice	1235:1241	An HFHSD resulted in a significant increase in the BA pool and fecal BA excretion in WT mice but not in DKO mice.
34558859	12	32	theme	primary	1709:1715	arg1	composition					1653:1663	altered composition	1645:1663	altered composition	1645:1663	Conclusion: The HFHSD itself causes the change of gut microbiota due to HFHSD, and the altered composition or concentration of BAs by HFHSD is not the primary factor.
34558859	12	32	theme	primary	1709:1715	arg1	factor					1717:1722	the primary factor	1705:1722	the primary factor	1705:1722	Conclusion: The HFHSD itself causes the change of gut microbiota due to HFHSD, and the altered composition or concentration of BAs by HFHSD is not the primary factor.
34558859	12	32	theme	primary	1709:1715	arg1	Conclusion					1558:1567	Conclusion	1558:1567	Conclusion: The HFHSD itself causes the change of gut microbiota due to HFHSD, and the altered composition or concentration of BAs by HFHSD is not the primary factor.	1558:1723	Conclusion: The HFHSD itself causes the change of gut microbiota due to HFHSD, and the altered composition or concentration of BAs by HFHSD is not the primary factor.
34558859	12	32	theme	primary	1709:1715	arg1	concentration					1668:1680	concentration	1668:1680	concentration	1668:1680	Conclusion: The HFHSD itself causes the change of gut microbiota due to HFHSD, and the altered composition or concentration of BAs by HFHSD is not the primary factor.
34558859	8	33	from	profiles	1079:1086	arg1	models					1123:1128	the two mouse models	1109:1128	the two mouse models	1109:1128	Despite the marked difference in the fecal BA composition, the profiles of gut microbiota in the two mouse models were quite similar.
34558859	4	34	theme	gut	493:495	arg1	microbiota					497:506	the changed gut microbiota	481:506	the changed gut microbiota	481:506	Then, we studied the effects of the changed gut microbiota on BA composition and liver function.
34558859	3	35	theme	BAs	398:400	arg1	roles					389:393	the roles	385:393	the roles of BAs in the HFHSD-induced change of gut microbiota	385:446	We first aimed to clarify the roles of BAs in the HFHSD-induced change of gut microbiota.
34558859	4	36	from	effects	470:476	arg1	composition					514:524	BA composition	511:524	BA composition	511:524	Then, we studied the effects of the changed gut microbiota on BA composition and liver function.
34558859	4	36	from	effects	470:476	arg1	function					536:543	liver function	530:543	liver function	530:543	Then, we studied the effects of the changed gut microbiota on BA composition and liver function.
34558859	8	37	theme	marked	1028:1033	arg1	difference					1035:1044	the marked difference	1024:1044	the marked difference in the fecal BA composition	1024:1072	Despite the marked difference in the fecal BA composition, the profiles of gut microbiota in the two mouse models were quite similar.
34558859	5	38	dep	wild-type	551:559	arg1	WT					562:563	WT	562:563	WT	562:563	Male wild-type (WT) and human-like Cyp2a12/Cyp2c70 double knockout (DKO) mice derived from C57BL/6J were fed with normal chow or HFHSD for 4 weeks.
34558859	13	39	theme	human-like	1853:1862	arg1	composition					1879:1889	human-like hydrophobic BA composition	1853:1889	human-like hydrophobic BA composition	1853:1889	On the contrary, the gut microbiota formed by HFHSD affects BA composition and ameliorates liver injury in the mouse model with human-like hydrophobic BA composition.
34558859	3	40	theme	gut	433:435	arg1	microbiota					437:446	gut microbiota	433:446	gut microbiota	433:446	We first aimed to clarify the roles of BAs in the HFHSD-induced change of gut microbiota.
34558859	6	41	theme	gene	751:754	arg1	sequencing					756:765	fecal 16S ribosomal RNA gene sequencing	727:765	fecal 16S ribosomal RNA gene sequencing	727:765	Gut microbiomes were analyzed by fecal 16S ribosomal RNA gene sequencing, and BA composition was determined by liquid chromatography-tandem mass spectrometry.
34558859	13	42	theme	BA	1876:1877	arg1	composition					1879:1889	human-like hydrophobic BA composition	1853:1889	human-like hydrophobic BA composition	1853:1889	On the contrary, the gut microbiota formed by HFHSD affects BA composition and ameliorates liver injury in the mouse model with human-like hydrophobic BA composition.
34558859	2	43	theme	changes	298:304	arg1	mechanism					285:293	the mechanism	281:293	the mechanism of changes in both by HFHSD	281:321	Because gut microbiota and BAs could influence each other, the mechanism of changes in both by HFHSD is complicated and remains unclear.
34558859	8	44	theme	fecal	1053:1057	arg1	composition					1062:1072	the fecal BA composition	1049:1072	the fecal BA composition	1049:1072	Despite the marked difference in the fecal BA composition, the profiles of gut microbiota in the two mouse models were quite similar.
34558859	6	45	theme	ribosomal	737:745	arg1	sequencing					756:765	fecal 16S ribosomal RNA gene sequencing	727:765	fecal 16S ribosomal RNA gene sequencing	727:765	Gut microbiomes were analyzed by fecal 16S ribosomal RNA gene sequencing, and BA composition was determined by liquid chromatography-tandem mass spectrometry.
34558859	12	46	theme	altered	1645:1651	arg1	composition					1653:1663	altered composition	1645:1663	altered composition	1645:1663	Conclusion: The HFHSD itself causes the change of gut microbiota due to HFHSD, and the altered composition or concentration of BAs by HFHSD is not the primary factor.
34558859	12	46	theme	altered	1645:1651	arg1	factor					1717:1722	the primary factor	1705:1722	the primary factor	1705:1722	Conclusion: The HFHSD itself causes the change of gut microbiota due to HFHSD, and the altered composition or concentration of BAs by HFHSD is not the primary factor.
34558859	12	46	theme	altered	1645:1651	arg1	Conclusion					1558:1567	Conclusion	1558:1567	Conclusion: The HFHSD itself causes the change of gut microbiota due to HFHSD, and the altered composition or concentration of BAs by HFHSD is not the primary factor.	1558:1723	Conclusion: The HFHSD itself causes the change of gut microbiota due to HFHSD, and the altered composition or concentration of BAs by HFHSD is not the primary factor.
34558859	12	46	theme	altered	1645:1651	arg1	concentration					1668:1680	concentration	1668:1680	concentration	1668:1680	Conclusion: The HFHSD itself causes the change of gut microbiota due to HFHSD, and the altered composition or concentration of BAs by HFHSD is not the primary factor.
34558859	4	47	theme	liver	530:534	arg1	function					536:543	liver function	530:543	liver function	530:543	Then, we studied the effects of the changed gut microbiota on BA composition and liver function.
34558859	11	48	located	observed	1536:1543	arg1	mice					1552:1555	DKO mice	1548:1555	DKO mice	1548:1555	In addition, the HFHSD-induced change of gut microbiota inhibited BA deconjugation and 7α-dehydroxylation in both types of mice, which improved chronic liver injury observed in DKO mice.
34558859	11	48	located	observed	1536:1543	arg2	injury					1529:1534	chronic liver injury	1515:1534	chronic liver injury observed in DKO mice	1515:1555	In addition, the HFHSD-induced change of gut microbiota inhibited BA deconjugation and 7α-dehydroxylation in both types of mice, which improved chronic liver injury observed in DKO mice.
34558859	6	49	theme	chromatography-tandem	812:832	arg1	spectrometry					839:850	liquid chromatography-tandem mass spectrometry	805:850	liquid chromatography-tandem mass spectrometry	805:850	Gut microbiomes were analyzed by fecal 16S ribosomal RNA gene sequencing, and BA composition was determined by liquid chromatography-tandem mass spectrometry.
34558859	2	50	from	changes	298:304	arg1	both					309:312	both	309:312	both	309:312	Because gut microbiota and BAs could influence each other, the mechanism of changes in both by HFHSD is complicated and remains unclear.
34558859	11	51	theme	liver	1523:1527	arg1	injury					1529:1534	chronic liver injury	1515:1534	chronic liver injury observed in DKO mice	1515:1555	In addition, the HFHSD-induced change of gut microbiota inhibited BA deconjugation and 7α-dehydroxylation in both types of mice, which improved chronic liver injury observed in DKO mice.
34558859	5	52	theme	Cyp2a12/Cyp2c70	581:595	arg1	mice					619:622	Male wild-type (WT) and human-like Cyp2a12/Cyp2c70 double knockout (DKO) mice	546:622	Male wild-type (WT) and human-like Cyp2a12/Cyp2c70 double knockout (DKO) mice derived from C57BL/6J	546:644	Male wild-type (WT) and human-like Cyp2a12/Cyp2c70 double knockout (DKO) mice derived from C57BL/6J were fed with normal chow or HFHSD for 4 weeks.
34558859	10	53	theme	microbial	1273:1281	arg1	composition					1283:1293	microbial composition	1273:1293	microbial composition in the two mouse models	1273:1317	However, microbial composition in the two mouse models was drastically but similarly changed by the HFHSD.
34558859	2	54	from	both	309:312	arg1	mechanism					285:293	the mechanism	281:293	the mechanism of changes in both by HFHSD	281:321	Because gut microbiota and BAs could influence each other, the mechanism of changes in both by HFHSD is complicated and remains unclear.
34558859	5	55	theme	wild-type	551:559	arg1	mice					619:622	Male wild-type (WT) and human-like Cyp2a12/Cyp2c70 double knockout (DKO) mice	546:622	Male wild-type (WT) and human-like Cyp2a12/Cyp2c70 double knockout (DKO) mice derived from C57BL/6J	546:644	Male wild-type (WT) and human-like Cyp2a12/Cyp2c70 double knockout (DKO) mice derived from C57BL/6J were fed with normal chow or HFHSD for 4 weeks.
34558859	11	56	theme	gut	1412:1414	arg1	microbiota					1416:1425	gut microbiota	1412:1425	gut microbiota	1412:1425	In addition, the HFHSD-induced change of gut microbiota inhibited BA deconjugation and 7α-dehydroxylation in both types of mice, which improved chronic liver injury observed in DKO mice.
34558859	8	57	theme	microbiota	1095:1104	arg1	profiles					1079:1086	the profiles	1075:1086	the profiles of gut microbiota in the two mouse models	1075:1128	Despite the marked difference in the fecal BA composition, the profiles of gut microbiota in the two mouse models were quite similar.
34558859	8	57	theme	microbiota	1095:1104	arg1	similar					1141:1147	similar	1141:1147	similar	1141:1147	Despite the marked difference in the fecal BA composition, the profiles of gut microbiota in the two mouse models were quite similar.
34558859	12	58	theme	gut	1608:1610	arg1	microbiota					1612:1621	gut microbiota	1608:1621	gut microbiota	1608:1621	Conclusion: The HFHSD itself causes the change of gut microbiota due to HFHSD, and the altered composition or concentration of BAs by HFHSD is not the primary factor.
34558859	11	59	theme	DKO	1548:1550	arg1	mice					1552:1555	DKO mice	1548:1555	DKO mice	1548:1555	In addition, the HFHSD-induced change of gut microbiota inhibited BA deconjugation and 7α-dehydroxylation in both types of mice, which improved chronic liver injury observed in DKO mice.
34558859	6	60	theme	Gut	694:696	arg1	microbiomes					698:708	Gut microbiomes	694:708	Gut microbiomes	694:708	Gut microbiomes were analyzed by fecal 16S ribosomal RNA gene sequencing, and BA composition was determined by liquid chromatography-tandem mass spectrometry.
34558859	1	61	theme	gut	178:180	arg1	microbiota					182:191	gut microbiota	178:191	gut microbiota	178:191	Western-style high-fat/high-sucrose diet (HFHSD) changes gut microbiota and bile acid (BA) profiles.
34558859	13	62	theme	BA	1785:1786	arg1	composition					1788:1798	BA composition	1785:1798	BA composition	1785:1798	On the contrary, the gut microbiota formed by HFHSD affects BA composition and ameliorates liver injury in the mouse model with human-like hydrophobic BA composition.
34558859	7	63	theme	acids	1009:1013	arg1	proportions					961:971	proportions	961:971	proportions of chenodeoxycholic and lithocholic acids	961:1013	The DKO mice exhibited significantly reduced fecal BA concentration, lacked muricholic acids, and increased proportions of chenodeoxycholic and lithocholic acids.
34558859	0	64	with	Model	76:80	arg1	Composition					108:118	Human-Like Bile Acid Composition	87:118	Human-Like Bile Acid Composition	87:118	Western Diet Changes Gut Microbiota and Ameliorates Liver Injury in a Mouse Model with Human-Like Bile Acid Composition.
34558859	8	65	theme	mouse	1117:1121	arg1	models					1123:1128	the two mouse models	1109:1128	the two mouse models	1109:1128	Despite the marked difference in the fecal BA composition, the profiles of gut microbiota in the two mouse models were quite similar.
34558859	5	66	theme	knockout	604:611	arg1	mice					619:622	Male wild-type (WT) and human-like Cyp2a12/Cyp2c70 double knockout (DKO) mice	546:622	Male wild-type (WT) and human-like Cyp2a12/Cyp2c70 double knockout (DKO) mice derived from C57BL/6J	546:644	Male wild-type (WT) and human-like Cyp2a12/Cyp2c70 double knockout (DKO) mice derived from C57BL/6J were fed with normal chow or HFHSD for 4 weeks.
34558859	13	67	theme	gut	1746:1748	arg1	microbiota					1750:1759	the gut microbiota	1742:1759	the gut microbiota formed by HFHSD	1742:1775	On the contrary, the gut microbiota formed by HFHSD affects BA composition and ameliorates liver injury in the mouse model with human-like hydrophobic BA composition.
34558859	5	68	theme	DKO	614:616	arg1	mice					619:622	Male wild-type (WT) and human-like Cyp2a12/Cyp2c70 double knockout (DKO) mice	546:622	Male wild-type (WT) and human-like Cyp2a12/Cyp2c70 double knockout (DKO) mice derived from C57BL/6J	546:644	Male wild-type (WT) and human-like Cyp2a12/Cyp2c70 double knockout (DKO) mice derived from C57BL/6J were fed with normal chow or HFHSD for 4 weeks.
34558859	9	69	theme	significant	1173:1183	arg1	increase					1185:1192	a significant increase	1171:1192	a significant increase in the BA pool	1171:1207	An HFHSD resulted in a significant increase in the BA pool and fecal BA excretion in WT mice but not in DKO mice.
34558859	8	70	from	difference	1035:1044	arg1	composition					1062:1072	the fecal BA composition	1049:1072	the fecal BA composition	1049:1072	Despite the marked difference in the fecal BA composition, the profiles of gut microbiota in the two mouse models were quite similar.
34558859	2	71	theme	gut	230:232	arg1	microbiota					234:243	gut microbiota	230:243	gut microbiota	230:243	Because gut microbiota and BAs could influence each other, the mechanism of changes in both by HFHSD is complicated and remains unclear.
34558859	1	72	theme	high-fat/high-sucrose	135:155	arg1	diet					157:160	Western-style high-fat/high-sucrose diet	121:160	Western-style high-fat/high-sucrose diet (HFHSD)	121:168	Western-style high-fat/high-sucrose diet (HFHSD) changes gut microbiota and bile acid (BA) profiles.
34558859	1	72	theme	high-fat/high-sucrose	135:155	arg1	HFHSD					163:167	HFHSD	163:167	HFHSD	163:167	Western-style high-fat/high-sucrose diet (HFHSD) changes gut microbiota and bile acid (BA) profiles.
34558859	11	73	theme	HFHSD-induced	1388:1400	arg1	change					1402:1407	the HFHSD-induced change	1384:1407	the HFHSD-induced change of gut microbiota	1384:1425	In addition, the HFHSD-induced change of gut microbiota inhibited BA deconjugation and 7α-dehydroxylation in both types of mice, which improved chronic liver injury observed in DKO mice.
34558859	0	74	theme	Mouse	70:74	arg1	Model					76:80	a Mouse Model	68:80	a Mouse Model with Human-Like Bile Acid Composition	68:118	Western Diet Changes Gut Microbiota and Ameliorates Liver Injury in a Mouse Model with Human-Like Bile Acid Composition.
34558859	9	75	theme	BA	1201:1202	arg1	pool					1204:1207	the BA pool	1197:1207	the BA pool	1197:1207	An HFHSD resulted in a significant increase in the BA pool and fecal BA excretion in WT mice but not in DKO mice.
34558859	7	76	theme	fecal	898:902	arg1	concentration					907:919	significantly reduced fecal BA concentration	876:919	significantly reduced fecal BA concentration	876:919	The DKO mice exhibited significantly reduced fecal BA concentration, lacked muricholic acids, and increased proportions of chenodeoxycholic and lithocholic acids.
34558859	5	77	attach	derived	624:630	arg2	mice					619:622	Male wild-type (WT) and human-like Cyp2a12/Cyp2c70 double knockout (DKO) mice	546:622	Male wild-type (WT) and human-like Cyp2a12/Cyp2c70 double knockout (DKO) mice derived from C57BL/6J	546:644	Male wild-type (WT) and human-like Cyp2a12/Cyp2c70 double knockout (DKO) mice derived from C57BL/6J were fed with normal chow or HFHSD for 4 weeks.
34558859	5	77	attach	derived	624:630	arg1	C57BL/6J					637:644	C57BL/6J	637:644	C57BL/6J	637:644	Male wild-type (WT) and human-like Cyp2a12/Cyp2c70 double knockout (DKO) mice derived from C57BL/6J were fed with normal chow or HFHSD for 4 weeks.
34558859	0	78	theme	Bile	98:101	arg1	Composition					108:118	Human-Like Bile Acid Composition	87:118	Human-Like Bile Acid Composition	87:118	Western Diet Changes Gut Microbiota and Ameliorates Liver Injury in a Mouse Model with Human-Like Bile Acid Composition.
34558859	9	79	theme	BA	1219:1220	arg1	excretion					1222:1230	fecal BA excretion	1213:1230	fecal BA excretion	1213:1230	An HFHSD resulted in a significant increase in the BA pool and fecal BA excretion in WT mice but not in DKO mice.
34558859	13	80	with	model	1842:1846	arg1	composition					1879:1889	human-like hydrophobic BA composition	1853:1889	human-like hydrophobic BA composition	1853:1889	On the contrary, the gut microbiota formed by HFHSD affects BA composition and ameliorates liver injury in the mouse model with human-like hydrophobic BA composition.
34558859	4	81	theme	changed	485:491	arg1	microbiota					497:506	the changed gut microbiota	481:506	the changed gut microbiota	481:506	Then, we studied the effects of the changed gut microbiota on BA composition and liver function.
34558859	0	82	theme	Gut	21:23	arg1	Microbiota					25:34	Gut Microbiota	21:34	Gut Microbiota	21:34	Western Diet Changes Gut Microbiota and Ameliorates Liver Injury in a Mouse Model with Human-Like Bile Acid Composition.
34558859	4	83	theme	microbiota	497:506	arg1	effects					470:476	the effects	466:476	the effects of the changed gut microbiota on BA composition and liver function	466:543	Then, we studied the effects of the changed gut microbiota on BA composition and liver function.
34558859	3	84	theme	HFHSD-induced	409:421	arg1	change					423:428	the HFHSD-induced change	405:428	the HFHSD-induced change of gut microbiota	405:446	We first aimed to clarify the roles of BAs in the HFHSD-induced change of gut microbiota.
34558859	1	85	dep	microbiota	182:191	arg1	profiles					212:219	profiles	212:219	profiles	212:219	Western-style high-fat/high-sucrose diet (HFHSD) changes gut microbiota and bile acid (BA) profiles.
34558859	5	86	theme	normal	660:665	arg1	chow					667:670	normal chow	660:670	normal chow	660:670	Male wild-type (WT) and human-like Cyp2a12/Cyp2c70 double knockout (DKO) mice derived from C57BL/6J were fed with normal chow or HFHSD for 4 weeks.
34558859	0	87	theme	Liver	52:56	arg1	Injury					58:63	Liver Injury	52:63	Liver Injury in a Mouse Model with Human-Like Bile Acid Composition	52:118	Western Diet Changes Gut Microbiota and Ameliorates Liver Injury in a Mouse Model with Human-Like Bile Acid Composition.
34558859	9	88	theme	DKO	1254:1256	arg1	mice					1258:1261	DKO mice	1254:1261	DKO mice	1254:1261	An HFHSD resulted in a significant increase in the BA pool and fecal BA excretion in WT mice but not in DKO mice.
34558859	13	89	theme	mouse	1836:1840	arg1	model					1842:1846	the mouse model	1832:1846	the mouse model with human-like hydrophobic BA composition	1832:1889	On the contrary, the gut microbiota formed by HFHSD affects BA composition and ameliorates liver injury in the mouse model with human-like hydrophobic BA composition.
34558859	13	90	theme	hydrophobic	1864:1874	arg1	composition					1879:1889	human-like hydrophobic BA composition	1853:1889	human-like hydrophobic BA composition	1853:1889	On the contrary, the gut microbiota formed by HFHSD affects BA composition and ameliorates liver injury in the mouse model with human-like hydrophobic BA composition.
34558859	3	91	theme	microbiota	437:446	arg1	change					423:428	the HFHSD-induced change	405:428	the HFHSD-induced change of gut microbiota	405:446	We first aimed to clarify the roles of BAs in the HFHSD-induced change of gut microbiota.
34558859	8	92	theme	BA	1059:1060	arg1	composition					1062:1072	the fecal BA composition	1049:1072	the fecal BA composition	1049:1072	Despite the marked difference in the fecal BA composition, the profiles of gut microbiota in the two mouse models were quite similar.
34558859	6	93	theme	RNA	747:749	arg1	sequencing					756:765	fecal 16S ribosomal RNA gene sequencing	727:765	fecal 16S ribosomal RNA gene sequencing	727:765	Gut microbiomes were analyzed by fecal 16S ribosomal RNA gene sequencing, and BA composition was determined by liquid chromatography-tandem mass spectrometry.
34558859	12	94	theme	BAs	1685:1687	arg1	composition					1653:1663	altered composition	1645:1663	altered composition	1645:1663	Conclusion: The HFHSD itself causes the change of gut microbiota due to HFHSD, and the altered composition or concentration of BAs by HFHSD is not the primary factor.
34558859	12	94	theme	BAs	1685:1687	arg1	factor					1717:1722	the primary factor	1705:1722	the primary factor	1705:1722	Conclusion: The HFHSD itself causes the change of gut microbiota due to HFHSD, and the altered composition or concentration of BAs by HFHSD is not the primary factor.
34558859	12	94	theme	BAs	1685:1687	arg1	Conclusion					1558:1567	Conclusion	1558:1567	Conclusion: The HFHSD itself causes the change of gut microbiota due to HFHSD, and the altered composition or concentration of BAs by HFHSD is not the primary factor.	1558:1723	Conclusion: The HFHSD itself causes the change of gut microbiota due to HFHSD, and the altered composition or concentration of BAs by HFHSD is not the primary factor.
34558859	12	94	theme	BAs	1685:1687	arg1	concentration					1668:1680	concentration	1668:1680	concentration	1668:1680	Conclusion: The HFHSD itself causes the change of gut microbiota due to HFHSD, and the altered composition or concentration of BAs by HFHSD is not the primary factor.
34558859	10	95	from	composition	1283:1293	arg1	models					1312:1317	the two mouse models	1298:1317	the two mouse models	1298:1317	However, microbial composition in the two mouse models was drastically but similarly changed by the HFHSD.
34558859	11	96	theme	mice	1494:1497	arg1	types					1485:1489	both types	1480:1489	both types of mice, which improved chronic liver injury observed in DKO mice	1480:1555	In addition, the HFHSD-induced change of gut microbiota inhibited BA deconjugation and 7α-dehydroxylation in both types of mice, which improved chronic liver injury observed in DKO mice.
34558859	9	97	from	increase	1185:1192	arg1	pool					1204:1207	the BA pool	1197:1207	the BA pool	1197:1207	An HFHSD resulted in a significant increase in the BA pool and fecal BA excretion in WT mice but not in DKO mice.
34558859	9	97	from	increase	1185:1192	arg1	mice					1258:1261	DKO mice	1254:1261	DKO mice	1254:1261	An HFHSD resulted in a significant increase in the BA pool and fecal BA excretion in WT mice but not in DKO mice.
34558859	9	97	from	increase	1185:1192	arg1	mice					1238:1241	WT mice	1235:1241	WT mice	1235:1241	An HFHSD resulted in a significant increase in the BA pool and fecal BA excretion in WT mice but not in DKO mice.
34558859	6	98	theme	16S	733:735	arg1	sequencing					756:765	fecal 16S ribosomal RNA gene sequencing	727:765	fecal 16S ribosomal RNA gene sequencing	727:765	Gut microbiomes were analyzed by fecal 16S ribosomal RNA gene sequencing, and BA composition was determined by liquid chromatography-tandem mass spectrometry.
34558859	3	99	from	roles	389:393	arg1	change					423:428	the HFHSD-induced change	405:428	the HFHSD-induced change of gut microbiota	405:446	We first aimed to clarify the roles of BAs in the HFHSD-induced change of gut microbiota.
33978026	7	0	theme	astonishing	1198:1208	arg1	reproducibility					1210:1224	astonishing reproducibility	1198:1224	astonishing reproducibility regarding onset and final mechanical properties	1198:1272	The second phase is initiated by bacterial cellulose excretion and shows astonishing reproducibility regarding onset and final mechanical properties.
33978026	1	1	theme	sugared	151:157	arg1	tea					159:161	sugared tea	151:161	sugared tea	151:161	Kombucha is a traditional beverage obtained by the fermentation of sugared tea by a symbiotic culture of bacteria and yeast which has recently re-emerged as a popular lifestyle product with potential health benefits.
33978026	4	2	theme	in	752:753	arg1	determination					760:772	the transient in situ determination	738:772	the transient in situ determination of kombucha biofilm growth	738:799	Here, we use interfacial shear rheology (ISR) for the transient in situ determination of kombucha biofilm growth directly at the interface.
33978026	6	3	theme	initial	980:986	arg1	adsorption					988:997	the initial adsorption	976:997	the initial adsorption of bacteria at the air-water interface	976:1036	The first phase can be attributed to the initial adsorption of bacteria at the air-water interface and shows great variability, probably due to varying bacteria content and composition.
33978026	0	4	theme	properties	72:81	arg1	measurement					18:28	Transient in situ measurement	0:28	Transient in situ measurement of kombucha biofilm growth and mechanical properties	0:81	Transient in situ measurement of kombucha biofilm growth and mechanical properties.
33978026	1	5	theme	tea	159:161	arg1	fermentation					135:146	the fermentation	131:146	the fermentation of sugared tea	131:161	Kombucha is a traditional beverage obtained by the fermentation of sugared tea by a symbiotic culture of bacteria and yeast which has recently re-emerged as a popular lifestyle product with potential health benefits.
33978026	6	6	dep	bacteria	1091:1098	arg1	content					1100:1106	content	1100:1106	content	1100:1106	The first phase can be attributed to the initial adsorption of bacteria at the air-water interface and shows great variability, probably due to varying bacteria content and composition.
33978026	6	6	dep	bacteria	1091:1098	arg1	bacteria					1091:1098	varying bacteria content and composition	1083:1122	varying bacteria content and composition	1083:1122	The first phase can be attributed to the initial adsorption of bacteria at the air-water interface and shows great variability, probably due to varying bacteria content and composition.
33978026	6	6	dep	bacteria	1091:1098	arg1	composition					1112:1122	composition	1112:1122	composition	1112:1122	The first phase can be attributed to the initial adsorption of bacteria at the air-water interface and shows great variability, probably due to varying bacteria content and composition.
33978026	3	7	from	interest	510:517	arg1	kombucha					522:529	kombucha	522:529	kombucha	522:529	Despite the growing industrial and technological interest in kombucha, current characterization techniques rely on the periodic sampling of tea broth or biofilm and ex situ analysis of its biochemical or microbial composition.
33978026	3	8	theme	characterization	540:555	arg1	techniques					557:566	current characterization techniques	532:566	current characterization techniques	532:566	Despite the growing industrial and technological interest in kombucha, current characterization techniques rely on the periodic sampling of tea broth or biofilm and ex situ analysis of its biochemical or microbial composition.
33978026	8	9	theme	cellulose	1382:1390	arg1	production					1392:1401	bacterial cellulose production	1372:1401	bacterial cellulose production	1372:1401	Hence, ISR qualifies as a new in situ characterization technique for kombucha biofilm growth and bacterial cellulose production.
33978026	1	10	theme	popular	243:249	arg1	product					261:267	a popular lifestyle product	241:267	a popular lifestyle product with potential health benefits	241:298	Kombucha is a traditional beverage obtained by the fermentation of sugared tea by a symbiotic culture of bacteria and yeast which has recently re-emerged as a popular lifestyle product with potential health benefits.
33978026	5	11	theme	growth	924:929	arg1	phases					931:936	clearly distinguishable growth phases	900:936	clearly distinguishable growth phases	900:936	ISR revealed that kombucha biofilm formation is a two step process with clearly distinguishable growth phases.
33978026	2	12	with	excretion	392:400	arg1	crystallinity					446:458	crystallinity	446:458	crystallinity	446:458	The characteristic feature of kombucha is the formation of a cellulosic biofilm due to the excretion of bacterial cellulose with high purity and crystallinity.
33978026	2	12	with	excretion	392:400	arg1	purity					435:440	high purity	430:440	high purity	430:440	The characteristic feature of kombucha is the formation of a cellulosic biofilm due to the excretion of bacterial cellulose with high purity and crystallinity.
33978026	8	13	theme	biofilm	1353:1359	arg1	growth					1361:1366	kombucha biofilm growth	1344:1366	kombucha biofilm growth	1344:1366	Hence, ISR qualifies as a new in situ characterization technique for kombucha biofilm growth and bacterial cellulose production.
33978026	7	14	theme	final	1246:1250	arg1	properties					1263:1272	final mechanical properties	1246:1272	final mechanical properties	1246:1272	The second phase is initiated by bacterial cellulose excretion and shows astonishing reproducibility regarding onset and final mechanical properties.
33978026	3	15	theme	technological	496:508	arg1	interest					510:517	the growing industrial and technological interest	469:517	the growing industrial and technological interest in kombucha	469:529	Despite the growing industrial and technological interest in kombucha, current characterization techniques rely on the periodic sampling of tea broth or biofilm and ex situ analysis of its biochemical or microbial composition.
33978026	1	16	theme	lifestyle	251:259	arg1	product					261:267	a popular lifestyle product	241:267	a popular lifestyle product with potential health benefits	241:298	Kombucha is a traditional beverage obtained by the fermentation of sugared tea by a symbiotic culture of bacteria and yeast which has recently re-emerged as a popular lifestyle product with potential health benefits.
33978026	7	17	theme	cellulose	1168:1176	arg1	excretion					1178:1186	bacterial cellulose excretion	1158:1186	bacterial cellulose excretion	1158:1186	The second phase is initiated by bacterial cellulose excretion and shows astonishing reproducibility regarding onset and final mechanical properties.
33978026	4	18	theme	interfacial	701:711	arg1	ISR					729:731	ISR	729:731	ISR	729:731	Here, we use interfacial shear rheology (ISR) for the transient in situ determination of kombucha biofilm growth directly at the interface.
33978026	4	18	theme	interfacial	701:711	arg1	rheology					719:726	interfacial shear rheology	701:726	interfacial shear rheology (ISR) for the transient in situ determination of kombucha biofilm growth	701:799	Here, we use interfacial shear rheology (ISR) for the transient in situ determination of kombucha biofilm growth directly at the interface.
33978026	1	19	theme	symbiotic	168:176	arg1	culture					178:184	a symbiotic culture	166:184	a symbiotic culture of bacteria and yeast	166:206	Kombucha is a traditional beverage obtained by the fermentation of sugared tea by a symbiotic culture of bacteria and yeast which has recently re-emerged as a popular lifestyle product with potential health benefits.
33978026	5	20	theme	step	882:885	arg1	process					887:893	a two step process	876:893	a two step process with clearly distinguishable growth phases	876:936	ISR revealed that kombucha biofilm formation is a two step process with clearly distinguishable growth phases.
33978026	5	20	theme	step	882:885	arg1	formation					863:871	kombucha biofilm formation	846:871	kombucha biofilm formation	846:871	ISR revealed that kombucha biofilm formation is a two step process with clearly distinguishable growth phases.
33978026	7	21	theme	second	1129:1134	arg1	phase					1136:1140	The second phase	1125:1140	The second phase	1125:1140	The second phase is initiated by bacterial cellulose excretion and shows astonishing reproducibility regarding onset and final mechanical properties.
33978026	2	22	theme	high	430:433	arg1	purity					435:440	high purity	430:440	high purity	430:440	The characteristic feature of kombucha is the formation of a cellulosic biofilm due to the excretion of bacterial cellulose with high purity and crystallinity.
33978026	5	23	theme	kombucha	846:853	arg1	process					887:893	a two step process	876:893	a two step process with clearly distinguishable growth phases	876:936	ISR revealed that kombucha biofilm formation is a two step process with clearly distinguishable growth phases.
33978026	5	23	theme	kombucha	846:853	arg1	formation					863:871	kombucha biofilm formation	846:871	kombucha biofilm formation	846:871	ISR revealed that kombucha biofilm formation is a two step process with clearly distinguishable growth phases.
33978026	0	24	theme	in	10:11	arg1	measurement					18:28	Transient in situ measurement	0:28	Transient in situ measurement of kombucha biofilm growth and mechanical properties	0:81	Transient in situ measurement of kombucha biofilm growth and mechanical properties.
33978026	6	25	theme	first	943:947	arg1	phase					949:953	The first phase	939:953	The first phase	939:953	The first phase can be attributed to the initial adsorption of bacteria at the air-water interface and shows great variability, probably due to varying bacteria content and composition.
33978026	5	26	with	process	887:893	arg1	phases					931:936	clearly distinguishable growth phases	900:936	clearly distinguishable growth phases	900:936	ISR revealed that kombucha biofilm formation is a two step process with clearly distinguishable growth phases.
33978026	2	27	theme	characteristic	305:318	arg1	formation					347:355	the formation	343:355	the formation of a cellulosic biofilm due to the excretion of bacterial cellulose with high purity and crystallinity	343:458	The characteristic feature of kombucha is the formation of a cellulosic biofilm due to the excretion of bacterial cellulose with high purity and crystallinity.
33978026	2	27	theme	characteristic	305:318	arg1	feature					320:326	The characteristic feature	301:326	The characteristic feature of kombucha	301:338	The characteristic feature of kombucha is the formation of a cellulosic biofilm due to the excretion of bacterial cellulose with high purity and crystallinity.
33978026	2	28	theme	biofilm	373:379	arg1	formation					347:355	the formation	343:355	the formation of a cellulosic biofilm due to the excretion of bacterial cellulose with high purity and crystallinity	343:458	The characteristic feature of kombucha is the formation of a cellulosic biofilm due to the excretion of bacterial cellulose with high purity and crystallinity.
33978026	2	28	theme	biofilm	373:379	arg1	feature					320:326	The characteristic feature	301:326	The characteristic feature of kombucha	301:338	The characteristic feature of kombucha is the formation of a cellulosic biofilm due to the excretion of bacterial cellulose with high purity and crystallinity.
33978026	0	29	theme	Transient	0:8	arg1	measurement					18:28	Transient in situ measurement	0:28	Transient in situ measurement of kombucha biofilm growth and mechanical properties	0:81	Transient in situ measurement of kombucha biofilm growth and mechanical properties.
33978026	8	30	theme	situ	1308:1311	arg1	technique					1330:1338	a new in situ characterization technique	1299:1338	a new in situ characterization technique for kombucha biofilm growth and bacterial cellulose production	1299:1401	Hence, ISR qualifies as a new in situ characterization technique for kombucha biofilm growth and bacterial cellulose production.
33978026	4	31	theme	shear	713:717	arg1	ISR					729:731	ISR	729:731	ISR	729:731	Here, we use interfacial shear rheology (ISR) for the transient in situ determination of kombucha biofilm growth directly at the interface.
33978026	4	31	theme	shear	713:717	arg1	rheology					719:726	interfacial shear rheology	701:726	interfacial shear rheology (ISR) for the transient in situ determination of kombucha biofilm growth	701:799	Here, we use interfacial shear rheology (ISR) for the transient in situ determination of kombucha biofilm growth directly at the interface.
33978026	1	32	with	product	261:267	arg1	benefits					291:298	potential health benefits	274:298	potential health benefits	274:298	Kombucha is a traditional beverage obtained by the fermentation of sugared tea by a symbiotic culture of bacteria and yeast which has recently re-emerged as a popular lifestyle product with potential health benefits.
33978026	3	33	theme	periodic	580:587	arg1	sampling					589:596	the periodic sampling	576:596	the periodic sampling of tea broth or biofilm	576:620	Despite the growing industrial and technological interest in kombucha, current characterization techniques rely on the periodic sampling of tea broth or biofilm and ex situ analysis of its biochemical or microbial composition.
33978026	4	34	theme	transient	742:750	arg1	determination					760:772	the transient in situ determination	738:772	the transient in situ determination of kombucha biofilm growth	738:799	Here, we use interfacial shear rheology (ISR) for the transient in situ determination of kombucha biofilm growth directly at the interface.
33978026	2	35	theme	cellulosic	362:371	arg1	biofilm					373:379	a cellulosic biofilm	360:379	a cellulosic biofilm due to the excretion of bacterial cellulose with high purity and crystallinity	360:458	The characteristic feature of kombucha is the formation of a cellulosic biofilm due to the excretion of bacterial cellulose with high purity and crystallinity.
33978026	4	36	theme	biofilm	786:792	arg1	growth					794:799	kombucha biofilm growth	777:799	kombucha biofilm growth	777:799	Here, we use interfacial shear rheology (ISR) for the transient in situ determination of kombucha biofilm growth directly at the interface.
33978026	5	37	theme	distinguishable	908:922	arg1	phases					931:936	clearly distinguishable growth phases	900:936	clearly distinguishable growth phases	900:936	ISR revealed that kombucha biofilm formation is a two step process with clearly distinguishable growth phases.
33978026	8	38	theme	characterization	1313:1328	arg1	technique					1330:1338	a new in situ characterization technique	1299:1338	a new in situ characterization technique for kombucha biofilm growth and bacterial cellulose production	1299:1401	Hence, ISR qualifies as a new in situ characterization technique for kombucha biofilm growth and bacterial cellulose production.
33978026	2	39	theme	bacterial	405:413	arg1	cellulose					415:423	bacterial cellulose	405:423	bacterial cellulose	405:423	The characteristic feature of kombucha is the formation of a cellulosic biofilm due to the excretion of bacterial cellulose with high purity and crystallinity.
33978026	2	40	theme	cellulose	415:423	arg1	excretion					392:400	the excretion	388:400	the excretion of bacterial cellulose with high purity and crystallinity	388:458	The characteristic feature of kombucha is the formation of a cellulosic biofilm due to the excretion of bacterial cellulose with high purity and crystallinity.
33978026	1	41	theme	potential	274:282	arg1	benefits					291:298	potential health benefits	274:298	potential health benefits	274:298	Kombucha is a traditional beverage obtained by the fermentation of sugared tea by a symbiotic culture of bacteria and yeast which has recently re-emerged as a popular lifestyle product with potential health benefits.
33978026	1	42	theme	traditional	98:108	arg1	beverage					110:117	a traditional beverage	96:117	a traditional beverage obtained by the fermentation of sugared tea by a symbiotic culture of bacteria and yeast which has recently re-emerged as a popular lifestyle product with potential health benefits	96:298	Kombucha is a traditional beverage obtained by the fermentation of sugared tea by a symbiotic culture of bacteria and yeast which has recently re-emerged as a popular lifestyle product with potential health benefits.
33978026	1	42	theme	traditional	98:108	arg1	Kombucha					84:91	Kombucha	84:91	Kombucha	84:91	Kombucha is a traditional beverage obtained by the fermentation of sugared tea by a symbiotic culture of bacteria and yeast which has recently re-emerged as a popular lifestyle product with potential health benefits.
33978026	8	43	theme	bacterial	1372:1380	arg1	production					1392:1401	bacterial cellulose production	1372:1401	bacterial cellulose production	1372:1401	Hence, ISR qualifies as a new in situ characterization technique for kombucha biofilm growth and bacterial cellulose production.
33978026	1	44	theme	bacteria	189:196	arg1	culture					178:184	a symbiotic culture	166:184	a symbiotic culture of bacteria and yeast	166:206	Kombucha is a traditional beverage obtained by the fermentation of sugared tea by a symbiotic culture of bacteria and yeast which has recently re-emerged as a popular lifestyle product with potential health benefits.
33978026	0	45	theme	kombucha	33:40	arg1	growth					50:55	kombucha biofilm growth	33:55	kombucha biofilm growth	33:55	Transient in situ measurement of kombucha biofilm growth and mechanical properties.
33978026	1	46	theme	health	284:289	arg1	benefits					291:298	potential health benefits	274:298	potential health benefits	274:298	Kombucha is a traditional beverage obtained by the fermentation of sugared tea by a symbiotic culture of bacteria and yeast which has recently re-emerged as a popular lifestyle product with potential health benefits.
33978026	7	47	theme	mechanical	1252:1261	arg1	properties					1263:1272	final mechanical properties	1246:1272	final mechanical properties	1246:1272	The second phase is initiated by bacterial cellulose excretion and shows astonishing reproducibility regarding onset and final mechanical properties.
33978026	3	48	theme	tea	601:603	arg1	broth					605:609	tea broth	601:609	tea broth	601:609	Despite the growing industrial and technological interest in kombucha, current characterization techniques rely on the periodic sampling of tea broth or biofilm and ex situ analysis of its biochemical or microbial composition.
33978026	6	49	theme	air-water	1018:1026	arg1	interface					1028:1036	the air-water interface	1014:1036	the air-water interface	1014:1036	The first phase can be attributed to the initial adsorption of bacteria at the air-water interface and shows great variability, probably due to varying bacteria content and composition.
33978026	6	50	theme	great	1048:1052	arg1	variability					1054:1064	great variability	1048:1064	great variability	1048:1064	The first phase can be attributed to the initial adsorption of bacteria at the air-water interface and shows great variability, probably due to varying bacteria content and composition.
33978026	5	51	theme	biofilm	855:861	arg1	process					887:893	a two step process	876:893	a two step process with clearly distinguishable growth phases	876:936	ISR revealed that kombucha biofilm formation is a two step process with clearly distinguishable growth phases.
33978026	5	51	theme	biofilm	855:861	arg1	formation					863:871	kombucha biofilm formation	846:871	kombucha biofilm formation	846:871	ISR revealed that kombucha biofilm formation is a two step process with clearly distinguishable growth phases.
33978026	8	52	theme	in	1305:1306	arg1	technique					1330:1338	a new in situ characterization technique	1299:1338	a new in situ characterization technique for kombucha biofilm growth and bacterial cellulose production	1299:1401	Hence, ISR qualifies as a new in situ characterization technique for kombucha biofilm growth and bacterial cellulose production.
33978026	3	53	theme	broth	605:609	arg1	sampling					589:596	the periodic sampling	576:596	the periodic sampling of tea broth or biofilm	576:620	Despite the growing industrial and technological interest in kombucha, current characterization techniques rely on the periodic sampling of tea broth or biofilm and ex situ analysis of its biochemical or microbial composition.
33978026	3	53	theme	broth	605:609	arg1	analysis					634:641	ex situ analysis	626:641	ex situ analysis of its biochemical or microbial composition	626:685	Despite the growing industrial and technological interest in kombucha, current characterization techniques rely on the periodic sampling of tea broth or biofilm and ex situ analysis of its biochemical or microbial composition.
33978026	1	54	theme	yeast	202:206	arg1	culture					178:184	a symbiotic culture	166:184	a symbiotic culture of bacteria and yeast	166:206	Kombucha is a traditional beverage obtained by the fermentation of sugared tea by a symbiotic culture of bacteria and yeast which has recently re-emerged as a popular lifestyle product with potential health benefits.
33978026	0	55	theme	growth	50:55	arg1	measurement					18:28	Transient in situ measurement	0:28	Transient in situ measurement of kombucha biofilm growth and mechanical properties	0:81	Transient in situ measurement of kombucha biofilm growth and mechanical properties.
33978026	8	56	theme	kombucha	1344:1351	arg1	growth					1361:1366	kombucha biofilm growth	1344:1366	kombucha biofilm growth	1344:1366	Hence, ISR qualifies as a new in situ characterization technique for kombucha biofilm growth and bacterial cellulose production.
33978026	6	57	from	interface	1028:1036	arg1	adsorption					988:997	the initial adsorption	976:997	the initial adsorption of bacteria at the air-water interface	976:1036	The first phase can be attributed to the initial adsorption of bacteria at the air-water interface and shows great variability, probably due to varying bacteria content and composition.
33978026	3	58	theme	industrial	481:490	arg1	interest					510:517	the growing industrial and technological interest	469:517	the growing industrial and technological interest in kombucha	469:529	Despite the growing industrial and technological interest in kombucha, current characterization techniques rely on the periodic sampling of tea broth or biofilm and ex situ analysis of its biochemical or microbial composition.
33978026	0	59	theme	biofilm	42:48	arg1	growth					50:55	kombucha biofilm growth	33:55	kombucha biofilm growth	33:55	Transient in situ measurement of kombucha biofilm growth and mechanical properties.
33978026	3	60	theme	microbial	665:673	arg1	composition					675:685	its biochemical or microbial composition	646:685	its biochemical or microbial composition	646:685	Despite the growing industrial and technological interest in kombucha, current characterization techniques rely on the periodic sampling of tea broth or biofilm and ex situ analysis of its biochemical or microbial composition.
33978026	2	61	theme	kombucha	331:338	arg1	formation					347:355	the formation	343:355	the formation of a cellulosic biofilm due to the excretion of bacterial cellulose with high purity and crystallinity	343:458	The characteristic feature of kombucha is the formation of a cellulosic biofilm due to the excretion of bacterial cellulose with high purity and crystallinity.
33978026	2	61	theme	kombucha	331:338	arg1	feature					320:326	The characteristic feature	301:326	The characteristic feature of kombucha	301:338	The characteristic feature of kombucha is the formation of a cellulosic biofilm due to the excretion of bacterial cellulose with high purity and crystallinity.
33978026	4	62	theme	kombucha	777:784	arg1	growth					794:799	kombucha biofilm growth	777:799	kombucha biofilm growth	777:799	Here, we use interfacial shear rheology (ISR) for the transient in situ determination of kombucha biofilm growth directly at the interface.
33978026	3	63	theme	composition	675:685	arg1	sampling					589:596	the periodic sampling	576:596	the periodic sampling of tea broth or biofilm	576:620	Despite the growing industrial and technological interest in kombucha, current characterization techniques rely on the periodic sampling of tea broth or biofilm and ex situ analysis of its biochemical or microbial composition.
33978026	3	63	theme	composition	675:685	arg1	analysis					634:641	ex situ analysis	626:641	ex situ analysis of its biochemical or microbial composition	626:685	Despite the growing industrial and technological interest in kombucha, current characterization techniques rely on the periodic sampling of tea broth or biofilm and ex situ analysis of its biochemical or microbial composition.
33978026	4	64	theme	growth	794:799	arg1	determination					760:772	the transient in situ determination	738:772	the transient in situ determination of kombucha biofilm growth	738:799	Here, we use interfacial shear rheology (ISR) for the transient in situ determination of kombucha biofilm growth directly at the interface.
33978026	8	65	theme	new	1301:1303	arg1	technique					1330:1338	a new in situ characterization technique	1299:1338	a new in situ characterization technique for kombucha biofilm growth and bacterial cellulose production	1299:1401	Hence, ISR qualifies as a new in situ characterization technique for kombucha biofilm growth and bacterial cellulose production.
33978026	3	66	theme	current	532:538	arg1	techniques					557:566	current characterization techniques	532:566	current characterization techniques	532:566	Despite the growing industrial and technological interest in kombucha, current characterization techniques rely on the periodic sampling of tea broth or biofilm and ex situ analysis of its biochemical or microbial composition.
33978026	3	67	theme	biochemical	650:660	arg1	composition					675:685	its biochemical or microbial composition	646:685	its biochemical or microbial composition	646:685	Despite the growing industrial and technological interest in kombucha, current characterization techniques rely on the periodic sampling of tea broth or biofilm and ex situ analysis of its biochemical or microbial composition.
33978026	3	68	theme	ex	626:627	arg1	analysis					634:641	ex situ analysis	626:641	ex situ analysis of its biochemical or microbial composition	626:685	Despite the growing industrial and technological interest in kombucha, current characterization techniques rely on the periodic sampling of tea broth or biofilm and ex situ analysis of its biochemical or microbial composition.
33978026	3	69	theme	growing	473:479	arg1	interest					510:517	the growing industrial and technological interest	469:517	the growing industrial and technological interest in kombucha	469:529	Despite the growing industrial and technological interest in kombucha, current characterization techniques rely on the periodic sampling of tea broth or biofilm and ex situ analysis of its biochemical or microbial composition.
33978026	6	70	theme	bacteria	1002:1009	arg1	adsorption					988:997	the initial adsorption	976:997	the initial adsorption of bacteria at the air-water interface	976:1036	The first phase can be attributed to the initial adsorption of bacteria at the air-water interface and shows great variability, probably due to varying bacteria content and composition.
33978026	0	71	theme	mechanical	61:70	arg1	properties					72:81	mechanical properties	61:81	mechanical properties	61:81	Transient in situ measurement of kombucha biofilm growth and mechanical properties.
33978026	3	72	dep	ex	626:627	arg1	situ					629:632	situ	629:632	situ	629:632	Despite the growing industrial and technological interest in kombucha, current characterization techniques rely on the periodic sampling of tea broth or biofilm and ex situ analysis of its biochemical or microbial composition.
33978026	0	73	dep	in	10:11	arg1	situ					13:16	situ	13:16	situ	13:16	Transient in situ measurement of kombucha biofilm growth and mechanical properties.
33978026	6	74	theme	varying	1083:1089	arg1	content					1100:1106	content	1100:1106	content	1100:1106	The first phase can be attributed to the initial adsorption of bacteria at the air-water interface and shows great variability, probably due to varying bacteria content and composition.
33978026	6	74	theme	varying	1083:1089	arg1	bacteria					1091:1098	varying bacteria content and composition	1083:1122	varying bacteria content and composition	1083:1122	The first phase can be attributed to the initial adsorption of bacteria at the air-water interface and shows great variability, probably due to varying bacteria content and composition.
33978026	6	74	theme	varying	1083:1089	arg1	composition					1112:1122	composition	1112:1122	composition	1112:1122	The first phase can be attributed to the initial adsorption of bacteria at the air-water interface and shows great variability, probably due to varying bacteria content and composition.
33978026	3	75	theme	biofilm	614:620	arg1	sampling					589:596	the periodic sampling	576:596	the periodic sampling of tea broth or biofilm	576:620	Despite the growing industrial and technological interest in kombucha, current characterization techniques rely on the periodic sampling of tea broth or biofilm and ex situ analysis of its biochemical or microbial composition.
33978026	3	75	theme	biofilm	614:620	arg1	analysis					634:641	ex situ analysis	626:641	ex situ analysis of its biochemical or microbial composition	626:685	Despite the growing industrial and technological interest in kombucha, current characterization techniques rely on the periodic sampling of tea broth or biofilm and ex situ analysis of its biochemical or microbial composition.
33978026	4	76	dep	in	752:753	arg1	situ					755:758	situ	755:758	situ	755:758	Here, we use interfacial shear rheology (ISR) for the transient in situ determination of kombucha biofilm growth directly at the interface.
33978026	7	77	theme	bacterial	1158:1166	arg1	excretion					1178:1186	bacterial cellulose excretion	1158:1186	bacterial cellulose excretion	1158:1186	The second phase is initiated by bacterial cellulose excretion and shows astonishing reproducibility regarding onset and final mechanical properties.
33982283	5	0	theme	repair	822:827	arg1	study in					829:836	a cartilage repair study in	810:836	a cartilage repair study in which full-thickness chondral defects were created surgically in both stifles of seven Shetland ponies	810:939	Posttraumatic osteoarthritic samples were obtained from a cartilage repair study in which full-thickness chondral defects were created surgically in both stifles of seven Shetland ponies.
33982283	4	1	theme	equine	526:531	arg1	model					533:537	an equine model	523:537	an equine model	523:537	Here, we determine the potential of QDECT to evaluate tissue quality surrounding cartilage lesions in an equine model, hypothesizing that QDECT allows detection of posttraumatic degeneration by providing quantitative information on PG and water contents based on the partitions of cationic and nonionic agents in a contrast mixture.
33982283	11	2	theme	severity	1828:1835	arg1	diagnostics					1798:1808	the diagnostics	1794:1808	the diagnostics of the extent and severity of cartilage injuries	1794:1857	QDECT enables evaluation of posttraumatic changes surrounding a lesion and quantification of PG content, thus advancing the diagnostics of the extent and severity of cartilage injuries.
33982283	1	3	theme	cartilage	175:183	arg1	composition					185:195	cartilage composition	175:195	cartilage composition	175:195	To prevent the progression of posttraumatic osteoarthritis, assessment of cartilage composition is critical for effective treatment planning.
33982283	7	4	dep	experimental	1008:1019	arg1	n = 14					1022:1027	n = 14	1022:1027	n = 14	1022:1027	The experimental (n = 14) and control samples (n = 6) were immersed in the contrast agent mixture and the distributions of the agents were determined at various diffusion time points.
33982283	4	5	theme	quantitative	625:636	arg1	information					638:648	quantitative information	625:648	quantitative information on PG and water contents based on the partitions of cationic and nonionic agents in a contrast mixture	625:751	Here, we determine the potential of QDECT to evaluate tissue quality surrounding cartilage lesions in an equine model, hypothesizing that QDECT allows detection of posttraumatic degeneration by providing quantitative information on PG and water contents based on the partitions of cationic and nonionic agents in a contrast mixture.
33982283	7	6	theme	agents	1131:1136	arg1	distributions					1110:1122	the distributions	1106:1122	the distributions of the agents	1106:1136	The experimental (n = 14) and control samples (n = 6) were immersed in the contrast agent mixture and the distributions of the agents were determined at various diffusion time points.
33982283	6	7	theme	nonoperated	984:994	arg1	ponies					996:1001	three nonoperated ponies	978:1001	three nonoperated ponies	978:1001	Control samples were collected from three nonoperated ponies.
33982283	7	8	theme	control	1034:1040	arg1	n = 6					1051:1055	n = 6	1051:1055	n = 6	1051:1055	The experimental (n = 14) and control samples (n = 6) were immersed in the contrast agent mixture and the distributions of the agents were determined at various diffusion time points.
33982283	7	8	theme	control	1034:1040	arg1	samples					1042:1048	The experimental (n = 14) and control samples	1004:1048	The experimental (n = 14) and control samples (n = 6)	1004:1056	The experimental (n = 14) and control samples (n = 6) were immersed in the contrast agent mixture and the distributions of the agents were determined at various diffusion time points.
33982283	1	9	theme	composition	185:195	arg1	assessment					161:170	assessment	161:170	assessment of cartilage composition	161:195	To prevent the progression of posttraumatic osteoarthritis, assessment of cartilage composition is critical for effective treatment planning.
33982283	6	10	theme	Control	942:948	arg1	samples					950:956	Control samples	942:956	Control samples	942:956	Control samples were collected from three nonoperated ponies.
33982283	10	11	theme	contrast	1606:1613	arg1	agent					1615:1619	cationic contrast agent	1597:1619	cationic contrast agent	1597:1619	Significant Spearman's rank correlations were obtained at 20 and 24 h (ρ = 0.482-0.693) between the partition of cationic contrast agent, cartilage biomechanical properties, and PG content.
33982283	7	12	theme	diffusion	1165:1173	arg1	points					1180:1185	various diffusion time points	1157:1185	various diffusion time points	1157:1185	The experimental (n = 14) and control samples (n = 6) were immersed in the contrast agent mixture and the distributions of the agents were determined at various diffusion time points.
33982283	10	13	theme	properties	1646:1655	arg1	partition					1584:1592	the partition	1580:1592	the partition of cationic contrast agent, cartilage biomechanical properties, and PG content	1580:1671	Significant Spearman's rank correlations were obtained at 20 and 24 h (ρ = 0.482-0.693) between the partition of cationic contrast agent, cartilage biomechanical properties, and PG content.
33982283	4	14	from	information	638:648	arg1	PG					653:654	PG	653:654	PG	653:654	Here, we determine the potential of QDECT to evaluate tissue quality surrounding cartilage lesions in an equine model, hypothesizing that QDECT allows detection of posttraumatic degeneration by providing quantitative information on PG and water contents based on the partitions of cationic and nonionic agents in a contrast mixture.
33982283	4	14	from	information	638:648	arg1	contents					666:673	water contents	660:673	water contents	660:673	Here, we determine the potential of QDECT to evaluate tissue quality surrounding cartilage lesions in an equine model, hypothesizing that QDECT allows detection of posttraumatic degeneration by providing quantitative information on PG and water contents based on the partitions of cationic and nonionic agents in a contrast mixture.
33982283	11	15	theme	posttraumatic	1702:1714	arg1	changes					1716:1722	posttraumatic changes	1702:1722	posttraumatic changes surrounding a lesion	1702:1743	QDECT enables evaluation of posttraumatic changes surrounding a lesion and quantification of PG content, thus advancing the diagnostics of the extent and severity of cartilage injuries.
33982283	7	16	theme	time	1175:1178	arg1	points					1180:1185	various diffusion time points	1157:1185	various diffusion time points	1157:1185	The experimental (n = 14) and control samples (n = 6) were immersed in the contrast agent mixture and the distributions of the agents were determined at various diffusion time points.
33982283	4	17	theme	contrast	736:743	arg1	mixture					745:751	a contrast mixture	734:751	a contrast mixture	734:751	Here, we determine the potential of QDECT to evaluate tissue quality surrounding cartilage lesions in an equine model, hypothesizing that QDECT allows detection of posttraumatic degeneration by providing quantitative information on PG and water contents based on the partitions of cationic and nonionic agents in a contrast mixture.
33982283	5	18	theme	Shetland	925:932	arg1	ponies					934:939	seven Shetland ponies	919:939	seven Shetland ponies	919:939	Posttraumatic osteoarthritic samples were obtained from a cartilage repair study in which full-thickness chondral defects were created surgically in both stifles of seven Shetland ponies.
33982283	8	19	theme	dynamic	1224:1230	arg1	reference					1193:1201	a reference	1191:1201	a reference	1191:1201	As a reference, equilibrium moduli, dynamic moduli, and PG content were measured.
33982283	8	19	theme	dynamic	1224:1230	arg1	moduli					1216:1221	equilibrium moduli	1204:1221	equilibrium moduli	1204:1221	As a reference, equilibrium moduli, dynamic moduli, and PG content were measured.
33982283	8	19	theme	dynamic	1224:1230	arg1	moduli					1232:1237	dynamic moduli	1224:1237	dynamic moduli	1224:1237	As a reference, equilibrium moduli, dynamic moduli, and PG content were measured.
33982283	8	19	theme	dynamic	1224:1230	arg1	content					1247:1253	PG content	1244:1253	PG content	1244:1253	As a reference, equilibrium moduli, dynamic moduli, and PG content were measured.
33982283	4	20	theme	cartilage	502:510	arg1	lesions					512:518	cartilage lesions	502:518	cartilage lesions	502:518	Here, we determine the potential of QDECT to evaluate tissue quality surrounding cartilage lesions in an equine model, hypothesizing that QDECT allows detection of posttraumatic degeneration by providing quantitative information on PG and water contents based on the partitions of cationic and nonionic agents in a contrast mixture.
33982283	10	21	theme	rank	1507:1510	arg1	correlations					1512:1523	Significant Spearman's rank correlations	1484:1523	Significant Spearman's rank correlations	1484:1523	Significant Spearman's rank correlations were obtained at 20 and 24 h (ρ = 0.482-0.693) between the partition of cationic contrast agent, cartilage biomechanical properties, and PG content.
33982283	10	22	theme	cartilage	1622:1630	arg1	properties					1646:1655	cartilage biomechanical properties	1622:1655	cartilage biomechanical properties	1622:1655	Significant Spearman's rank correlations were obtained at 20 and 24 h (ρ = 0.482-0.693) between the partition of cationic contrast agent, cartilage biomechanical properties, and PG content.
33982283	2	23	theme	water	309:313	arg1	content					315:321	elevated water content	300:321	elevated water content	300:321	Posttraumatic changes include proteoglycan (PG) loss and elevated water content.
33982283	4	24	theme	cationic	702:709	arg1	agents					724:729	cationic and nonionic agents	702:729	cationic and nonionic agents	702:729	Here, we determine the potential of QDECT to evaluate tissue quality surrounding cartilage lesions in an equine model, hypothesizing that QDECT allows detection of posttraumatic degeneration by providing quantitative information on PG and water contents based on the partitions of cationic and nonionic agents in a contrast mixture.
33982283	9	25	from	differences	1282:1292	arg1	partitions					1308:1317	partitions	1308:1317	partitions	1308:1317	Significant differences (p < 0.05) in partitions between the experimental and control samples were demonstrated with cationic contrast agent at 30 min, 60 min, and 20 h, and with non-ionic agent at 60 and 120 min.
33982283	7	26	theme	various	1157:1163	arg1	points					1180:1185	various diffusion time points	1157:1185	various diffusion time points	1157:1185	The experimental (n = 14) and control samples (n = 6) were immersed in the contrast agent mixture and the distributions of the agents were determined at various diffusion time points.
33982283	2	27	theme	elevated	300:307	arg1	content					315:321	elevated water content	300:321	elevated water content	300:321	Posttraumatic changes include proteoglycan (PG) loss and elevated water content.
33982283	11	28	theme	extent	1817:1822	arg1	diagnostics					1798:1808	the diagnostics	1794:1808	the diagnostics of the extent and severity of cartilage injuries	1794:1857	QDECT enables evaluation of posttraumatic changes surrounding a lesion and quantification of PG content, thus advancing the diagnostics of the extent and severity of cartilage injuries.
33982283	5	29	theme	ponies	934:939	arg1	stifles					908:914	both stifles	903:914	both stifles of seven Shetland ponies	903:939	Posttraumatic osteoarthritic samples were obtained from a cartilage repair study in which full-thickness chondral defects were created surgically in both stifles of seven Shetland ponies.
33982283	0	30	theme	computed	14:21	arg1	tomography					23:32	Dual-contrast computed tomography	0:32	Dual-contrast computed tomography	0:32	Dual-contrast computed tomography enables detection of equine posttraumatic osteoarthritis in vitro.
33982283	9	31	dep	experimental	1331:1342	arg1	samples					1356:1362	samples	1356:1362	samples	1356:1362	Significant differences (p < 0.05) in partitions between the experimental and control samples were demonstrated with cationic contrast agent at 30 min, 60 min, and 20 h, and with non-ionic agent at 60 and 120 min.
33982283	10	32	theme	cationic	1597:1604	arg1	agent					1615:1619	cationic contrast agent	1597:1619	cationic contrast agent	1597:1619	Significant Spearman's rank correlations were obtained at 20 and 24 h (ρ = 0.482-0.693) between the partition of cationic contrast agent, cartilage biomechanical properties, and PG content.
33982283	9	33	theme	cationic	1387:1394	arg1	agent					1405:1409	cationic contrast agent	1387:1409	cationic contrast agent	1387:1409	Significant differences (p < 0.05) in partitions between the experimental and control samples were demonstrated with cationic contrast agent at 30 min, 60 min, and 20 h, and with non-ionic agent at 60 and 120 min.
33982283	11	34	theme	changes	1716:1722	arg1	quantification					1749:1762	quantification	1749:1762	quantification of PG content	1749:1776	QDECT enables evaluation of posttraumatic changes surrounding a lesion and quantification of PG content, thus advancing the diagnostics of the extent and severity of cartilage injuries.
33982283	11	34	theme	changes	1716:1722	arg1	evaluation					1688:1697	evaluation	1688:1697	evaluation of posttraumatic changes surrounding a lesion	1688:1743	QDECT enables evaluation of posttraumatic changes surrounding a lesion and quantification of PG content, thus advancing the diagnostics of the extent and severity of cartilage injuries.
33982283	4	35	theme	nonionic	715:722	arg1	agents					724:729	cationic and nonionic agents	702:729	cationic and nonionic agents	702:729	Here, we determine the potential of QDECT to evaluate tissue quality surrounding cartilage lesions in an equine model, hypothesizing that QDECT allows detection of posttraumatic degeneration by providing quantitative information on PG and water contents based on the partitions of cationic and nonionic agents in a contrast mixture.
33982283	0	36	theme	Dual-contrast	0:12	arg1	tomography					23:32	Dual-contrast computed tomography	0:32	Dual-contrast computed tomography	0:32	Dual-contrast computed tomography enables detection of equine posttraumatic osteoarthritis in vitro.
33982283	4	37	theme	posttraumatic	585:597	arg1	degeneration					599:610	posttraumatic degeneration	585:610	posttraumatic degeneration	585:610	Here, we determine the potential of QDECT to evaluate tissue quality surrounding cartilage lesions in an equine model, hypothesizing that QDECT allows detection of posttraumatic degeneration by providing quantitative information on PG and water contents based on the partitions of cationic and nonionic agents in a contrast mixture.
33982283	10	38	theme	Significant	1484:1494	arg1	correlations					1512:1523	Significant Spearman's rank correlations	1484:1523	Significant Spearman's rank correlations	1484:1523	Significant Spearman's rank correlations were obtained at 20 and 24 h (ρ = 0.482-0.693) between the partition of cationic contrast agent, cartilage biomechanical properties, and PG content.
33982283	11	39	theme	PG	1767:1768	arg1	content					1770:1776	PG content	1767:1776	PG content	1767:1776	QDECT enables evaluation of posttraumatic changes surrounding a lesion and quantification of PG content, thus advancing the diagnostics of the extent and severity of cartilage injuries.
33982283	4	40	from	partitions	688:697	arg1	mixture					745:751	a contrast mixture	734:751	a contrast mixture	734:751	Here, we determine the potential of QDECT to evaluate tissue quality surrounding cartilage lesions in an equine model, hypothesizing that QDECT allows detection of posttraumatic degeneration by providing quantitative information on PG and water contents based on the partitions of cationic and nonionic agents in a contrast mixture.
33982283	1	41	theme	effective	213:221	arg1	planning					233:240	effective treatment planning	213:240	effective treatment planning	213:240	To prevent the progression of posttraumatic osteoarthritis, assessment of cartilage composition is critical for effective treatment planning.
33982283	8	42	theme	PG	1244:1245	arg1	reference					1193:1201	a reference	1191:1201	a reference	1191:1201	As a reference, equilibrium moduli, dynamic moduli, and PG content were measured.
33982283	8	42	theme	PG	1244:1245	arg1	moduli					1216:1221	equilibrium moduli	1204:1221	equilibrium moduli	1204:1221	As a reference, equilibrium moduli, dynamic moduli, and PG content were measured.
33982283	8	42	theme	PG	1244:1245	arg1	moduli					1232:1237	dynamic moduli	1224:1237	dynamic moduli	1224:1237	As a reference, equilibrium moduli, dynamic moduli, and PG content were measured.
33982283	8	42	theme	PG	1244:1245	arg1	content					1247:1253	PG content	1244:1253	PG content	1244:1253	As a reference, equilibrium moduli, dynamic moduli, and PG content were measured.
33982283	9	43	theme	contrast	1396:1403	arg1	agent					1405:1409	cationic contrast agent	1387:1409	cationic contrast agent	1387:1409	Significant differences (p < 0.05) in partitions between the experimental and control samples were demonstrated with cationic contrast agent at 30 min, 60 min, and 20 h, and with non-ionic agent at 60 and 120 min.
33982283	7	44	theme	experimental	1008:1019	arg1	n = 6					1051:1055	n = 6	1051:1055	n = 6	1051:1055	The experimental (n = 14) and control samples (n = 6) were immersed in the contrast agent mixture and the distributions of the agents were determined at various diffusion time points.
33982283	7	44	theme	experimental	1008:1019	arg1	samples					1042:1048	The experimental (n = 14) and control samples	1004:1048	The experimental (n = 14) and control samples (n = 6)	1004:1056	The experimental (n = 14) and control samples (n = 6) were immersed in the contrast agent mixture and the distributions of the agents were determined at various diffusion time points.
33982283	5	45	theme	chondral	859:866	arg1	defects					868:874	full-thickness chondral defects	844:874	full-thickness chondral defects	844:874	Posttraumatic osteoarthritic samples were obtained from a cartilage repair study in which full-thickness chondral defects were created surgically in both stifles of seven Shetland ponies.
33982283	1	46	theme	treatment	223:231	arg1	planning					233:240	effective treatment planning	213:240	effective treatment planning	213:240	To prevent the progression of posttraumatic osteoarthritis, assessment of cartilage composition is critical for effective treatment planning.
33982283	10	47	theme	agent	1615:1619	arg1	partition					1584:1592	the partition	1580:1592	the partition of cationic contrast agent, cartilage biomechanical properties, and PG content	1580:1671	Significant Spearman's rank correlations were obtained at 20 and 24 h (ρ = 0.482-0.693) between the partition of cationic contrast agent, cartilage biomechanical properties, and PG content.
33982283	7	48	theme	agent	1088:1092	arg1	mixture					1094:1100	the contrast agent mixture	1075:1100	the contrast agent mixture	1075:1100	The experimental (n = 14) and control samples (n = 6) were immersed in the contrast agent mixture and the distributions of the agents were determined at various diffusion time points.
33982283	4	49	theme	tissue	475:480	arg1	quality					482:488	tissue quality	475:488	tissue quality surrounding cartilage lesions	475:518	Here, we determine the potential of QDECT to evaluate tissue quality surrounding cartilage lesions in an equine model, hypothesizing that QDECT allows detection of posttraumatic degeneration by providing quantitative information on PG and water contents based on the partitions of cationic and nonionic agents in a contrast mixture.
33982283	10	50	theme	PG	1662:1663	arg1	content					1665:1671	PG content	1662:1671	PG content	1662:1671	Significant Spearman's rank correlations were obtained at 20 and 24 h (ρ = 0.482-0.693) between the partition of cationic contrast agent, cartilage biomechanical properties, and PG content.
33982283	4	51	theme	degeneration	599:610	arg1	detection					572:580	detection	572:580	detection of posttraumatic degeneration	572:610	Here, we determine the potential of QDECT to evaluate tissue quality surrounding cartilage lesions in an equine model, hypothesizing that QDECT allows detection of posttraumatic degeneration by providing quantitative information on PG and water contents based on the partitions of cationic and nonionic agents in a contrast mixture.
33982283	5	52	theme	Posttraumatic	754:766	arg1	samples					783:789	Posttraumatic osteoarthritic samples	754:789	Posttraumatic osteoarthritic samples	754:789	Posttraumatic osteoarthritic samples were obtained from a cartilage repair study in which full-thickness chondral defects were created surgically in both stifles of seven Shetland ponies.
33982283	11	53	dep	extent	1817:1822	arg1	the					1813:1815	the	1813:1815	the	1813:1815	QDECT enables evaluation of posttraumatic changes surrounding a lesion and quantification of PG content, thus advancing the diagnostics of the extent and severity of cartilage injuries.
33982283	10	54	theme	content	1665:1671	arg1	partition					1584:1592	the partition	1580:1592	the partition of cationic contrast agent, cartilage biomechanical properties, and PG content	1580:1671	Significant Spearman's rank correlations were obtained at 20 and 24 h (ρ = 0.482-0.693) between the partition of cationic contrast agent, cartilage biomechanical properties, and PG content.
33982283	5	55	theme	full-thickness	844:857	arg1	defects					868:874	full-thickness chondral defects	844:874	full-thickness chondral defects	844:874	Posttraumatic osteoarthritic samples were obtained from a cartilage repair study in which full-thickness chondral defects were created surgically in both stifles of seven Shetland ponies.
33982283	5	56	theme	osteoarthritic	768:781	arg1	samples					783:789	Posttraumatic osteoarthritic samples	754:789	Posttraumatic osteoarthritic samples	754:789	Posttraumatic osteoarthritic samples were obtained from a cartilage repair study in which full-thickness chondral defects were created surgically in both stifles of seven Shetland ponies.
33982283	2	57	theme	proteoglycan	273:284	arg1	loss					291:294	proteoglycan (PG) loss	273:294	proteoglycan (PG) loss	273:294	Posttraumatic changes include proteoglycan (PG) loss and elevated water content.
33982283	0	58	theme	posttraumatic	62:74	arg1	osteoarthritis					76:89	equine posttraumatic osteoarthritis	55:89	equine posttraumatic osteoarthritis	55:89	Dual-contrast computed tomography enables detection of equine posttraumatic osteoarthritis in vitro.
33982283	3	59	theme	computed	349:356	arg1	QDECT					370:374	QDECT	370:374	QDECT	370:374	Quantitative dual-energy computed tomography (QDECT) provides a means to diagnose these changes.
33982283	3	59	theme	computed	349:356	arg1	tomography					358:367	Quantitative dual-energy computed tomography	324:367	Quantitative dual-energy computed tomography (QDECT)	324:375	Quantitative dual-energy computed tomography (QDECT) provides a means to diagnose these changes.
33982283	5	60	theme	cartilage	812:820	arg1	study in					829:836	a cartilage repair study in	810:836	a cartilage repair study in which full-thickness chondral defects were created surgically in both stifles of seven Shetland ponies	810:939	Posttraumatic osteoarthritic samples were obtained from a cartilage repair study in which full-thickness chondral defects were created surgically in both stifles of seven Shetland ponies.
33982283	7	61	theme	contrast	1079:1086	arg1	mixture					1094:1100	the contrast agent mixture	1075:1100	the contrast agent mixture	1075:1100	The experimental (n = 14) and control samples (n = 6) were immersed in the contrast agent mixture and the distributions of the agents were determined at various diffusion time points.
33982283	0	62	theme	equine	55:60	arg1	osteoarthritis					76:89	equine posttraumatic osteoarthritis	55:89	equine posttraumatic osteoarthritis	55:89	Dual-contrast computed tomography enables detection of equine posttraumatic osteoarthritis in vitro.
33982283	11	63	theme	injuries	1850:1857	arg1	severity					1828:1835	severity	1828:1835	severity	1828:1835	QDECT enables evaluation of posttraumatic changes surrounding a lesion and quantification of PG content, thus advancing the diagnostics of the extent and severity of cartilage injuries.
33982283	11	63	theme	injuries	1850:1857	arg1	extent					1817:1822	extent	1817:1822	extent	1817:1822	QDECT enables evaluation of posttraumatic changes surrounding a lesion and quantification of PG content, thus advancing the diagnostics of the extent and severity of cartilage injuries.
33982283	2	64	theme	Posttraumatic	243:255	arg1	changes					257:263	Posttraumatic changes	243:263	Posttraumatic changes	243:263	Posttraumatic changes include proteoglycan (PG) loss and elevated water content.
33982283	11	65	theme	content	1770:1776	arg1	quantification					1749:1762	quantification	1749:1762	quantification of PG content	1749:1776	QDECT enables evaluation of posttraumatic changes surrounding a lesion and quantification of PG content, thus advancing the diagnostics of the extent and severity of cartilage injuries.
33982283	11	65	theme	content	1770:1776	arg1	evaluation					1688:1697	evaluation	1688:1697	evaluation of posttraumatic changes surrounding a lesion	1688:1743	QDECT enables evaluation of posttraumatic changes surrounding a lesion and quantification of PG content, thus advancing the diagnostics of the extent and severity of cartilage injuries.
33982283	1	66	theme	posttraumatic	131:143	arg1	osteoarthritis					145:158	posttraumatic osteoarthritis	131:158	posttraumatic osteoarthritis	131:158	To prevent the progression of posttraumatic osteoarthritis, assessment of cartilage composition is critical for effective treatment planning.
33982283	3	67	theme	Quantitative	324:335	arg1	QDECT					370:374	QDECT	370:374	QDECT	370:374	Quantitative dual-energy computed tomography (QDECT) provides a means to diagnose these changes.
33982283	3	67	theme	Quantitative	324:335	arg1	tomography					358:367	Quantitative dual-energy computed tomography	324:367	Quantitative dual-energy computed tomography (QDECT)	324:375	Quantitative dual-energy computed tomography (QDECT) provides a means to diagnose these changes.
33982283	8	68	theme	equilibrium	1204:1214	arg1	reference					1193:1201	a reference	1191:1201	a reference	1191:1201	As a reference, equilibrium moduli, dynamic moduli, and PG content were measured.
33982283	8	68	theme	equilibrium	1204:1214	arg1	moduli					1216:1221	equilibrium moduli	1204:1221	equilibrium moduli	1204:1221	As a reference, equilibrium moduli, dynamic moduli, and PG content were measured.
33982283	8	68	theme	equilibrium	1204:1214	arg1	moduli					1232:1237	dynamic moduli	1224:1237	dynamic moduli	1224:1237	As a reference, equilibrium moduli, dynamic moduli, and PG content were measured.
33982283	8	68	theme	equilibrium	1204:1214	arg1	content					1247:1253	PG content	1244:1253	PG content	1244:1253	As a reference, equilibrium moduli, dynamic moduli, and PG content were measured.
33982283	1	69	theme	osteoarthritis	145:158	arg1	progression					116:126	the progression	112:126	the progression of posttraumatic osteoarthritis	112:158	To prevent the progression of posttraumatic osteoarthritis, assessment of cartilage composition is critical for effective treatment planning.
33982283	3	70	theme	dual-energy	337:347	arg1	QDECT					370:374	QDECT	370:374	QDECT	370:374	Quantitative dual-energy computed tomography (QDECT) provides a means to diagnose these changes.
33982283	3	70	theme	dual-energy	337:347	arg1	tomography					358:367	Quantitative dual-energy computed tomography	324:367	Quantitative dual-energy computed tomography (QDECT)	324:375	Quantitative dual-energy computed tomography (QDECT) provides a means to diagnose these changes.
33982283	9	71	theme	non-ionic	1449:1457	arg1	agent					1459:1463	non-ionic agent	1449:1463	non-ionic agent	1449:1463	Significant differences (p < 0.05) in partitions between the experimental and control samples were demonstrated with cationic contrast agent at 30 min, 60 min, and 20 h, and with non-ionic agent at 60 and 120 min.
33982283	11	72	theme	cartilage	1840:1848	arg1	injuries					1850:1857	cartilage injuries	1840:1857	cartilage injuries	1840:1857	QDECT enables evaluation of posttraumatic changes surrounding a lesion and quantification of PG content, thus advancing the diagnostics of the extent and severity of cartilage injuries.
33982283	9	73	from	120 min	1475:1481	arg1	30 min					1414:1419	30 min	1414:1419	30 min	1414:1419	Significant differences (p < 0.05) in partitions between the experimental and control samples were demonstrated with cationic contrast agent at 30 min, 60 min, and 20 h, and with non-ionic agent at 60 and 120 min.
33982283	4	74	theme	QDECT	457:461	arg1	potential					444:452	the potential	440:452	the potential of QDECT to evaluate tissue quality surrounding cartilage lesions in an equine model	440:537	Here, we determine the potential of QDECT to evaluate tissue quality surrounding cartilage lesions in an equine model, hypothesizing that QDECT allows detection of posttraumatic degeneration by providing quantitative information on PG and water contents based on the partitions of cationic and nonionic agents in a contrast mixture.
33982283	9	75	theme	Significant	1270:1280	arg1	p < 0.05					1295:1302	p < 0.05	1295:1302	p < 0.05	1295:1302	Significant differences (p < 0.05) in partitions between the experimental and control samples were demonstrated with cationic contrast agent at 30 min, 60 min, and 20 h, and with non-ionic agent at 60 and 120 min.
33982283	9	75	theme	Significant	1270:1280	arg1	differences					1282:1292	Significant differences	1270:1292	Significant differences (p < 0.05) in partitions between the experimental and control samples	1270:1362	Significant differences (p < 0.05) in partitions between the experimental and control samples were demonstrated with cationic contrast agent at 30 min, 60 min, and 20 h, and with non-ionic agent at 60 and 120 min.
33982283	0	76	theme	osteoarthritis	76:89	arg1	detection					42:50	detection	42:50	detection of equine posttraumatic osteoarthritis	42:89	Dual-contrast computed tomography enables detection of equine posttraumatic osteoarthritis in vitro.
33982283	10	77	theme	biomechanical	1632:1644	arg1	properties					1646:1655	cartilage biomechanical properties	1622:1655	cartilage biomechanical properties	1622:1655	Significant Spearman's rank correlations were obtained at 20 and 24 h (ρ = 0.482-0.693) between the partition of cationic contrast agent, cartilage biomechanical properties, and PG content.
33982283	9	78	from	60	1468:1469	arg1	30 min					1414:1419	30 min	1414:1419	30 min	1414:1419	Significant differences (p < 0.05) in partitions between the experimental and control samples were demonstrated with cationic contrast agent at 30 min, 60 min, and 20 h, and with non-ionic agent at 60 and 120 min.
33982283	4	79	theme	agents	724:729	arg1	partitions					688:697	the partitions	684:697	the partitions of cationic and nonionic agents in a contrast mixture	684:751	Here, we determine the potential of QDECT to evaluate tissue quality surrounding cartilage lesions in an equine model, hypothesizing that QDECT allows detection of posttraumatic degeneration by providing quantitative information on PG and water contents based on the partitions of cationic and nonionic agents in a contrast mixture.
33982283	4	80	theme	water	660:664	arg1	contents					666:673	water contents	660:673	water contents	660:673	Here, we determine the potential of QDECT to evaluate tissue quality surrounding cartilage lesions in an equine model, hypothesizing that QDECT allows detection of posttraumatic degeneration by providing quantitative information on PG and water contents based on the partitions of cationic and nonionic agents in a contrast mixture.
32353494	8	0	theme	stellate	987:994	arg1	line					1001:1004	human hepatic stellate cell line	973:1004	human hepatic stellate cell line LX2 damage	973:1015	For carbon tetrachloride (CCl4) induced human hepatic stellate cell line LX2 damage, GelMA/OHA/Gal-CS/TA200 hydrogel could significantly improve the survival of LX2 cells.
32353494	3	1	theme	hepatocytes	543:553	arg1	regeneration					555:566	hepatocytes regeneration	543:566	hepatocytes regeneration	543:566	Fe (III)@TA microspheres served as carrier to achieve sustained release of IGF-2 to promote hepatocytes regeneration.
32353494	1	2	theme	used	103:106	arg1	materials					108:116	the most commonly used materials	85:116	the most commonly used materials in liver tissue engineering	85:144	As one of the most commonly used materials in liver tissue engineering, hydrogel has received much attention in recent years.
32353494	6	3	dep	prepared	779:786	arg1	suitable					829:836	suitable	829:836	suitable	829:836	The hydrogel we prepared had a storage modulus of 1100 Pa and was suitable for migration of hepatocytes.
32353494	6	3	dep	prepared	779:786	arg1	had					788:790	had	788:790	had a storage modulus of 1100 Pa	788:819	The hydrogel we prepared had a storage modulus of 1100 Pa and was suitable for migration of hepatocytes.
32353494	8	4	theme	hepatic	979:985	arg1	line					1001:1004	human hepatic stellate cell line	973:1004	human hepatic stellate cell line LX2 damage	973:1015	For carbon tetrachloride (CCl4) induced human hepatic stellate cell line LX2 damage, GelMA/OHA/Gal-CS/TA200 hydrogel could significantly improve the survival of LX2 cells.
32353494	3	5	theme	IGF-2	526:530	arg1	release					515:521	sustained release	505:521	sustained release of IGF-2	505:530	Fe (III)@TA microspheres served as carrier to achieve sustained release of IGF-2 to promote hepatocytes regeneration.
32353494	2	6	theme	acid	278:281	arg1	hydrogel					352:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel	227:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes	227:448	In this work, we prepared a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes.
32353494	10	7	theme	new	1331:1333	arg1	hydrogels					1304:1312	the GelMA/OHA/Gal-CS/TA200 hydrogels	1277:1312	the GelMA/OHA/Gal-CS/TA200 hydrogels	1277:1312	In summary, the GelMA/OHA/Gal-CS/TA200 hydrogels could be used as new tissue engineering scaffolds for the construction of artificial livers.
32353494	10	7	theme	new	1331:1333	arg1	scaffolds					1354:1362	new tissue engineering scaffolds	1331:1362	new tissue engineering scaffolds for the construction of artificial livers	1331:1404	In summary, the GelMA/OHA/Gal-CS/TA200 hydrogels could be used as new tissue engineering scaffolds for the construction of artificial livers.
32353494	6	8	theme	hepatocytes	855:865	arg1	migration					842:850	migration	842:850	migration of hepatocytes	842:865	The hydrogel we prepared had a storage modulus of 1100 Pa and was suitable for migration of hepatocytes.
32353494	0	9	theme	injury	57:62	arg1	treatment					64:72	liver injury treatment	51:72	liver injury treatment	51:72	Fe (III)@TA@IGF-2 microspheres loaded hydrogel for liver injury treatment.
32353494	1	10	from	materials	108:116	arg1	engineering					134:144	liver tissue engineering	121:144	liver tissue engineering	121:144	As one of the most commonly used materials in liver tissue engineering, hydrogel has received much attention in recent years.
32353494	0	11	theme	liver	51:55	arg1	injury					57:62	liver injury	51:62	liver injury treatment	51:72	Fe (III)@TA@IGF-2 microspheres loaded hydrogel for liver injury treatment.
32353494	4	12	from	receptor	624:631	arg1	surface					648:654	the surface	644:654	the surface of hepatocytes	644:669	Galactose ligands could bind to the asialoglycoprotein receptor (ASGPR) on the surface of hepatocytes.
32353494	4	13	theme	asialoglycoprotein	605:622	arg1	receptor					624:631	asialoglycoprotein receptor	605:631	the asialoglycoprotein receptor (ASGPR) on the surface of hepatocytes	601:669	Galactose ligands could bind to the asialoglycoprotein receptor (ASGPR) on the surface of hepatocytes.
32353494	4	13	theme	asialoglycoprotein	605:622	arg1	ASGPR					634:638	ASGPR	634:638	ASGPR	634:638	Galactose ligands could bind to the asialoglycoprotein receptor (ASGPR) on the surface of hepatocytes.
32353494	4	14	theme	Galactose	569:577	arg1	ligands					579:585	Galactose ligands	569:585	Galactose ligands	569:585	Galactose ligands could bind to the asialoglycoprotein receptor (ASGPR) on the surface of hepatocytes.
32353494	2	15	theme	growth	386:391	arg1	factor					393:398	insulin-like growth factor 2	373:400	insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes	373:448	In this work, we prepared a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes.
32353494	2	15	theme	growth	386:391	arg1	IGF-2					403:407	IGF-2	403:407	IGF-2	403:407	In this work, we prepared a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes.
32353494	9	16	theme	LX2	1162:1164	arg1	cells					1166:1170	LX2 cells	1162:1170	LX2 cells treated with hydrogel	1162:1192	The expression of HNF-4α and transferrin was detected in LX2 cells treated with hydrogel, indicating that the specific function of the liver was also restored.
32353494	1	17	theme	tissue	127:132	arg1	engineering					134:144	liver tissue engineering	121:144	liver tissue engineering	121:144	As one of the most commonly used materials in liver tissue engineering, hydrogel has received much attention in recent years.
32353494	2	18	theme	Gal-CS	314:319	arg1	hydrogel					352:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel	227:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes	227:448	In this work, we prepared a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes.
32353494	2	19	theme	IGF-2	334:338	arg1	hydrogel					352:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel	227:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes	227:448	In this work, we prepared a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes.
32353494	2	20	theme	TA	331:332	arg1	hydrogel					352:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel	227:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes	227:448	In this work, we prepared a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes.
32353494	9	21	located	detected	1150:1157	arg2	expression					1109:1118	The expression	1105:1118	The expression of HNF-4α and transferrin	1105:1144	The expression of HNF-4α and transferrin was detected in LX2 cells treated with hydrogel, indicating that the specific function of the liver was also restored.
32353494	9	21	located	detected	1150:1157	arg1	cells					1166:1170	LX2 cells	1162:1170	LX2 cells treated with hydrogel	1162:1192	The expression of HNF-4α and transferrin was detected in LX2 cells treated with hydrogel, indicating that the specific function of the liver was also restored.
32353494	1	22	theme	liver	121:125	arg1	engineering					134:144	liver tissue engineering	121:144	liver tissue engineering	121:144	As one of the most commonly used materials in liver tissue engineering, hydrogel has received much attention in recent years.
32353494	2	23	theme	@	333:333	arg1	hydrogel					352:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel	227:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes	227:448	In this work, we prepared a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes.
32353494	9	24	theme	HNF-4α	1123:1128	arg1	expression					1109:1118	The expression	1105:1118	The expression of HNF-4α and transferrin	1105:1144	The expression of HNF-4α and transferrin was detected in LX2 cells treated with hydrogel, indicating that the specific function of the liver was also restored.
32353494	8	25	theme	human	973:977	arg1	line					1001:1004	human hepatic stellate cell line	973:1004	human hepatic stellate cell line LX2 damage	973:1015	For carbon tetrachloride (CCl4) induced human hepatic stellate cell line LX2 damage, GelMA/OHA/Gal-CS/TA200 hydrogel could significantly improve the survival of LX2 cells.
32353494	2	26	theme	chitosan	304:311	arg1	hydrogel					352:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel	227:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes	227:448	In this work, we prepared a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes.
32353494	0	27	theme	Fe	0:1	arg1	microspheres					18:29	Fe (III)@TA@IGF-2 microspheres	0:29	Fe (III)@TA@IGF-2 microspheres	0:29	Fe (III)@TA@IGF-2 microspheres loaded hydrogel for liver injury treatment.
32353494	8	28	theme	LX2	1094:1096	arg1	cells					1098:1102	LX2 cells	1094:1102	LX2 cells	1094:1102	For carbon tetrachloride (CCl4) induced human hepatic stellate cell line LX2 damage, GelMA/OHA/Gal-CS/TA200 hydrogel could significantly improve the survival of LX2 cells.
32353494	2	29	theme	methacrylate	237:248	arg1	hydrogel					352:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel	227:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes	227:448	In this work, we prepared a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes.
32353494	2	30	theme	/galactosylated	288:302	arg1	hydrogel					352:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel	227:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes	227:448	In this work, we prepared a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes.
32353494	2	31	theme	@	330:330	arg1	hydrogel					352:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel	227:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes	227:448	In this work, we prepared a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes.
32353494	8	32	theme	LX2	1006:1008	arg1	damage					1010:1015	human hepatic stellate cell line LX2 damage	973:1015	human hepatic stellate cell line LX2 damage	973:1015	For carbon tetrachloride (CCl4) induced human hepatic stellate cell line LX2 damage, GelMA/OHA/Gal-CS/TA200 hydrogel could significantly improve the survival of LX2 cells.
32353494	10	33	theme	engineering	1342:1352	arg1	hydrogels					1304:1312	the GelMA/OHA/Gal-CS/TA200 hydrogels	1277:1312	the GelMA/OHA/Gal-CS/TA200 hydrogels	1277:1312	In summary, the GelMA/OHA/Gal-CS/TA200 hydrogels could be used as new tissue engineering scaffolds for the construction of artificial livers.
32353494	10	33	theme	engineering	1342:1352	arg1	scaffolds					1354:1362	new tissue engineering scaffolds	1331:1362	new tissue engineering scaffolds for the construction of artificial livers	1331:1404	In summary, the GelMA/OHA/Gal-CS/TA200 hydrogels could be used as new tissue engineering scaffolds for the construction of artificial livers.
32353494	2	34	theme	gelatin	229:235	arg1	hydrogel					352:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel	227:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes	227:448	In this work, we prepared a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes.
32353494	5	35	theme	Galactosylated	672:685	arg1	chitosan					687:694	Galactosylated chitosan	672:694	Galactosylated chitosan	672:694	Galactosylated chitosan could significantly increase the specific function of hepatocytes.
32353494	10	36	theme	tissue	1335:1340	arg1	hydrogels					1304:1312	the GelMA/OHA/Gal-CS/TA200 hydrogels	1277:1312	the GelMA/OHA/Gal-CS/TA200 hydrogels	1277:1312	In summary, the GelMA/OHA/Gal-CS/TA200 hydrogels could be used as new tissue engineering scaffolds for the construction of artificial livers.
32353494	10	36	theme	tissue	1335:1340	arg1	scaffolds					1354:1362	new tissue engineering scaffolds	1331:1362	new tissue engineering scaffolds for the construction of artificial livers	1331:1404	In summary, the GelMA/OHA/Gal-CS/TA200 hydrogels could be used as new tissue engineering scaffolds for the construction of artificial livers.
32353494	1	37	theme	materials	108:116	arg1	one					78:80	one	78:80	one	78:80	As one of the most commonly used materials in liver tissue engineering, hydrogel has received much attention in recent years.
32353494	1	37	theme	materials	108:116	arg1	materials					108:116	the most commonly used materials	85:116	the most commonly used materials in liver tissue engineering	85:144	As one of the most commonly used materials in liver tissue engineering, hydrogel has received much attention in recent years.
32353494	10	38	used	used	1323:1326	arg2	hydrogels					1304:1312	the GelMA/OHA/Gal-CS/TA200 hydrogels	1277:1312	the GelMA/OHA/Gal-CS/TA200 hydrogels	1277:1312	In summary, the GelMA/OHA/Gal-CS/TA200 hydrogels could be used as new tissue engineering scaffolds for the construction of artificial livers.
32353494	10	38	used	used	1323:1326	arg2	scaffolds					1354:1362	new tissue engineering scaffolds	1331:1362	new tissue engineering scaffolds for the construction of artificial livers	1331:1404	In summary, the GelMA/OHA/Gal-CS/TA200 hydrogels could be used as new tissue engineering scaffolds for the construction of artificial livers.
32353494	0	39	theme	TA	9:10	arg1	microspheres					18:29	Fe (III)@TA@IGF-2 microspheres	0:29	Fe (III)@TA@IGF-2 microspheres	0:29	Fe (III)@TA@IGF-2 microspheres loaded hydrogel for liver injury treatment.
32353494	8	40	theme	carbon	937:942	arg1	CCl4					959:962	CCl4	959:962	CCl4	959:962	For carbon tetrachloride (CCl4) induced human hepatic stellate cell line LX2 damage, GelMA/OHA/Gal-CS/TA200 hydrogel could significantly improve the survival of LX2 cells.
32353494	8	40	theme	carbon	937:942	arg1	tetrachloride					944:956	carbon tetrachloride	937:956	carbon tetrachloride (CCl4)	937:963	For carbon tetrachloride (CCl4) induced human hepatic stellate cell line LX2 damage, GelMA/OHA/Gal-CS/TA200 hydrogel could significantly improve the survival of LX2 cells.
32353494	4	41	theme	hepatocytes	659:669	arg1	surface					648:654	the surface	644:654	the surface of hepatocytes	644:669	Galactose ligands could bind to the asialoglycoprotein receptor (ASGPR) on the surface of hepatocytes.
32353494	8	42	theme	cells	1098:1102	arg1	survival					1082:1089	the survival	1078:1089	the survival of LX2 cells	1078:1102	For carbon tetrachloride (CCl4) induced human hepatic stellate cell line LX2 damage, GelMA/OHA/Gal-CS/TA200 hydrogel could significantly improve the survival of LX2 cells.
32353494	2	43	theme	/Fe	321:323	arg1	hydrogel					352:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel	227:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes	227:448	In this work, we prepared a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes.
32353494	0	44	theme	@	8:8	arg1	microspheres					18:29	Fe (III)@TA@IGF-2 microspheres	0:29	Fe (III)@TA@IGF-2 microspheres	0:29	Fe (III)@TA@IGF-2 microspheres loaded hydrogel for liver injury treatment.
32353494	6	45	theme	1100 Pa	813:819	arg1	modulus					802:808	a storage modulus	792:808	a storage modulus of 1100 Pa	792:819	The hydrogel we prepared had a storage modulus of 1100 Pa and was suitable for migration of hepatocytes.
32353494	9	46	theme	liver	1240:1244	arg1	function					1224:1231	the specific function	1211:1231	the specific function of the liver	1211:1244	The expression of HNF-4α and transferrin was detected in LX2 cells treated with hydrogel, indicating that the specific function of the liver was also restored.
32353494	2	47	theme	insulin-like	373:384	arg1	factor					393:398	insulin-like growth factor 2	373:400	insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes	373:448	In this work, we prepared a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes.
32353494	2	47	theme	insulin-like	373:384	arg1	IGF-2					403:407	IGF-2	403:407	IGF-2	403:407	In this work, we prepared a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes.
32353494	0	48	theme	IGF-2	12:16	arg1	microspheres					18:29	Fe (III)@TA@IGF-2 microspheres	0:29	Fe (III)@TA@IGF-2 microspheres	0:29	Fe (III)@TA@IGF-2 microspheres loaded hydrogel for liver injury treatment.
32353494	8	49	theme	line	1001:1004	arg1	damage					1010:1015	human hepatic stellate cell line LX2 damage	973:1015	human hepatic stellate cell line LX2 damage	973:1015	For carbon tetrachloride (CCl4) induced human hepatic stellate cell line LX2 damage, GelMA/OHA/Gal-CS/TA200 hydrogel could significantly improve the survival of LX2 cells.
32353494	2	50	theme	hepatocytes	438:448	arg1	regeneration					414:425	regeneration	414:425	regeneration of damaged hepatocytes	414:448	In this work, we prepared a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes.
32353494	10	51	theme	livers	1399:1404	arg1	construction					1372:1383	the construction	1368:1383	the construction of artificial livers	1368:1404	In summary, the GelMA/OHA/Gal-CS/TA200 hydrogels could be used as new tissue engineering scaffolds for the construction of artificial livers.
32353494	2	52	theme	hyaluronic	267:276	arg1	hydrogel					352:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel	227:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes	227:448	In this work, we prepared a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes.
32353494	0	53	theme	@	11:11	arg1	microspheres					18:29	Fe (III)@TA@IGF-2 microspheres	0:29	Fe (III)@TA@IGF-2 microspheres	0:29	Fe (III)@TA@IGF-2 microspheres loaded hydrogel for liver injury treatment.
32353494	9	54	theme	specific	1215:1222	arg1	function					1224:1231	the specific function	1211:1231	the specific function of the liver	1211:1244	The expression of HNF-4α and transferrin was detected in LX2 cells treated with hydrogel, indicating that the specific function of the liver was also restored.
32353494	2	55	theme	damaged	430:436	arg1	hepatocytes					438:448	damaged hepatocytes	430:448	damaged hepatocytes	430:448	In this work, we prepared a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes.
32353494	10	56	theme	artificial	1388:1397	arg1	livers					1399:1404	artificial livers	1388:1404	artificial livers	1388:1404	In summary, the GelMA/OHA/Gal-CS/TA200 hydrogels could be used as new tissue engineering scaffolds for the construction of artificial livers.
32353494	3	57	theme	Fe	451:452	arg1	microspheres					463:474	Fe (III)@TA microspheres	451:474	Fe (III)@TA microspheres	451:474	Fe (III)@TA microspheres served as carrier to achieve sustained release of IGF-2 to promote hepatocytes regeneration.
32353494	9	58	theme	transferrin	1134:1144	arg1	expression					1109:1118	The expression	1105:1118	The expression of HNF-4α and transferrin	1105:1144	The expression of HNF-4α and transferrin was detected in LX2 cells treated with hydrogel, indicating that the specific function of the liver was also restored.
32353494	2	59	theme	/oxidized	257:265	arg1	hydrogel					352:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel	227:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes	227:448	In this work, we prepared a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes.
32353494	6	60	theme	storage	794:800	arg1	modulus					802:808	a storage modulus	792:808	a storage modulus of 1100 Pa	792:819	The hydrogel we prepared had a storage modulus of 1100 Pa and was suitable for migration of hepatocytes.
32353494	7	61	theme	IGF-2	889:893	arg1	ratio					880:884	The release ratio	868:884	The release ratio of IGF-2	868:893	The release ratio of IGF-2 could reach up to 90% within 14 days.
32353494	8	62	theme	cell	996:999	arg1	line					1001:1004	human hepatic stellate cell line	973:1004	human hepatic stellate cell line LX2 damage	973:1015	For carbon tetrachloride (CCl4) induced human hepatic stellate cell line LX2 damage, GelMA/OHA/Gal-CS/TA200 hydrogel could significantly improve the survival of LX2 cells.
32353494	3	63	theme	TA	460:461	arg1	microspheres					463:474	Fe (III)@TA microspheres	451:474	Fe (III)@TA microspheres	451:474	Fe (III)@TA microspheres served as carrier to achieve sustained release of IGF-2 to promote hepatocytes regeneration.
32353494	7	64	theme	release	872:878	arg1	ratio					880:884	The release ratio	868:884	The release ratio of IGF-2	868:893	The release ratio of IGF-2 could reach up to 90% within 14 days.
32353494	1	65	theme	much	169:172	arg1	attention					174:182	much attention	169:182	much attention	169:182	As one of the most commonly used materials in liver tissue engineering, hydrogel has received much attention in recent years.
32353494	2	66	theme	GelMA	251:255	arg1	hydrogel					352:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel	227:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes	227:448	In this work, we prepared a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes.
32353494	8	67	theme	GelMA/OHA/Gal-CS/TA200	1018:1039	arg1	hydrogel					1041:1048	GelMA/OHA/Gal-CS/TA200 hydrogel	1018:1048	GelMA/OHA/Gal-CS/TA200 hydrogel	1018:1048	For carbon tetrachloride (CCl4) induced human hepatic stellate cell line LX2 damage, GelMA/OHA/Gal-CS/TA200 hydrogel could significantly improve the survival of LX2 cells.
32353494	4	68	gly	asialoglycoprotein	605:622	arg1	asialoglycoprotein					605:622	asialoglycoprotein receptor	605:631	the asialoglycoprotein receptor (ASGPR) on the surface of hepatocytes	601:669	Galactose ligands could bind to the asialoglycoprotein receptor (ASGPR) on the surface of hepatocytes.
32353494	3	69	theme	sustained	505:513	arg1	release					515:521	sustained release	505:521	sustained release of IGF-2	505:530	Fe (III)@TA microspheres served as carrier to achieve sustained release of IGF-2 to promote hepatocytes regeneration.
32353494	5	70	theme	hepatocytes	750:760	arg1	function					738:745	the specific function	725:745	the specific function of hepatocytes	725:760	Galactosylated chitosan could significantly increase the specific function of hepatocytes.
32353494	5	71	theme	specific	729:736	arg1	function					738:745	the specific function	725:745	the specific function of hepatocytes	725:760	Galactosylated chitosan could significantly increase the specific function of hepatocytes.
32353494	6	72	dep	hydrogel	767:774	arg1	we					776:777	we	776:777	we	776:777	The hydrogel we prepared had a storage modulus of 1100 Pa and was suitable for migration of hepatocytes.
32353494	7	73	dep	90	913:914	arg1	to					910:911	to	910:911	to	910:911	The release ratio of IGF-2 could reach up to 90% within 14 days.
32353494	3	74	theme	@	459:459	arg1	microspheres					463:474	Fe (III)@TA microspheres	451:474	Fe (III)@TA microspheres	451:474	Fe (III)@TA microspheres served as carrier to achieve sustained release of IGF-2 to promote hepatocytes regeneration.
32353494	2	75	theme	OHA	284:286	arg1	hydrogel					352:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel	227:359	a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes	227:448	In this work, we prepared a gelatin methacrylate (GelMA)/oxidized hyaluronic acid (OHA)/galactosylated chitosan (Gal-CS)/Fe (III)@TA@IGF-2 200 (TA200) hydrogel loaded with insulin-like growth factor 2 (IGF-2) for regeneration of damaged hepatocytes.
32353494	1	76	theme	recent	187:192	arg1	years					194:198	recent years	187:198	recent years	187:198	As one of the most commonly used materials in liver tissue engineering, hydrogel has received much attention in recent years.
34714845	5	0	theme	bracteal	745:752	arg1	nectar					757:762	bracteal EF nectar	745:762	bracteal EF nectar	745:762	This study is the first to test for systemically induced changes to the carbohydrate composition of bracteal EF nectar in response to foliar herbivory on G. hirsutum.
34714845	6	1	theme	EF	899:900	arg1	nectar					902:907	bracteal EF nectar	890:907	bracteal EF nectar	890:907	We found that foliar herbivory significantly increased the sucrose content of bracteal EF nectar while glucose and fructose remained unchanged.
34714845	5	2	from	herbivory	786:794	arg1	hirsutum					802:809	G. hirsutum	799:809	G. hirsutum	799:809	This study is the first to test for systemically induced changes to the carbohydrate composition of bracteal EF nectar in response to foliar herbivory on G. hirsutum.
34714845	1	3	theme	extrafloral	150:160	arg1	nectar					167:172	extrafloral (EF) nectar	150:172	extrafloral (EF) nectar on leaves (foliar) and reproductive structures (bracteal)	150:230	Cultivated cotton, such as Gossypium hirsutum L., produces extrafloral (EF) nectar on leaves (foliar) and reproductive structures (bracteal) as an indirect anti-herbivore defense.
34714845	1	3	theme	extrafloral	150:160	arg1	defense					262:268	an indirect anti-herbivore defense	235:268	an indirect anti-herbivore defense	235:268	Cultivated cotton, such as Gossypium hirsutum L., produces extrafloral (EF) nectar on leaves (foliar) and reproductive structures (bracteal) as an indirect anti-herbivore defense.
34714845	1	3	theme	extrafloral	150:160	arg1	EF					163:164	EF	163:164	EF	163:164	Cultivated cotton, such as Gossypium hirsutum L., produces extrafloral (EF) nectar on leaves (foliar) and reproductive structures (bracteal) as an indirect anti-herbivore defense.
34714845	9	4	from	increase	1495:1502	arg1	sucrose					1507:1513	sucrose	1507:1513	sucrose	1507:1513	The ants did not exhibit any preference for either solution, potentially because sucrose is a minor carbohydrate component in G. hirsutum EF nectar: total sugar content was not significantly affected by the increase in sucrose.
34714845	0	5	theme	cotton	83:88	arg1	nectar					73:78	bracteal extrafloral nectar	52:78	bracteal extrafloral nectar of cotton	52:88	Foliar herbivory increases sucrose concentration in bracteal extrafloral nectar of cotton.
34714845	6	6	theme	bracteal	890:897	arg1	nectar					902:907	bracteal EF nectar	890:907	bracteal EF nectar	890:907	We found that foliar herbivory significantly increased the sucrose content of bracteal EF nectar while glucose and fructose remained unchanged.
34714845	7	7	theme	herbivore-induced	1043:1059	arg1	increase					1061:1068	an herbivore-induced increase	1040:1068	an herbivore-induced increase to EF nectar caloric content	1040:1097	Sucrose content is known to influence ant foraging behavior and previous studies of an herbivore-induced increase to EF nectar caloric content found that it led to increased ant activity on the plant.
34714845	11	8	theme	Gossypium	1732:1740	arg1	typical					1721:1727	typical	1721:1727	typical	1721:1727	Further research is needed to determine whether an herbivore-induced increase in sucrose content is typical of Gossypium spp., and whether it constitutes a corollary of systemic sucrose induction, or a potentially adaptive mechanism which enhances ant attraction to the plant.
34714845	11	8	theme	Gossypium	1732:1740	arg1	increase					1690:1697	an herbivore-induced increase	1669:1697	an herbivore-induced increase in sucrose content	1669:1716	Further research is needed to determine whether an herbivore-induced increase in sucrose content is typical of Gossypium spp., and whether it constitutes a corollary of systemic sucrose induction, or a potentially adaptive mechanism which enhances ant attraction to the plant.
34714845	7	9	from	activity	1134:1141	arg1	plant					1150:1154	the plant	1146:1154	the plant	1146:1154	Sucrose content is known to influence ant foraging behavior and previous studies of an herbivore-induced increase to EF nectar caloric content found that it led to increased ant activity on the plant.
34714845	7	10	theme	Sucrose	956:962	arg1	content					964:970	Sucrose content	956:970	Sucrose content	956:970	Sucrose content is known to influence ant foraging behavior and previous studies of an herbivore-induced increase to EF nectar caloric content found that it led to increased ant activity on the plant.
34714845	10	11	theme	new	1548:1550	arg1	questions					1552:1560	new questions	1548:1560	new questions about cotton's inducible EF nectar responses to herbivory	1548:1618	Nonetheless, our findings raise new questions about cotton's inducible EF nectar responses to herbivory.
34714845	9	12	theme	sugar	1443:1447	arg1	content					1449:1455	total sugar content	1437:1455	total sugar content	1437:1455	The ants did not exhibit any preference for either solution, potentially because sucrose is a minor carbohydrate component in G. hirsutum EF nectar: total sugar content was not significantly affected by the increase in sucrose.
34714845	5	13	dep	herbivory	786:794	arg1	response					767:774	response	767:774	response	767:774	This study is the first to test for systemically induced changes to the carbohydrate composition of bracteal EF nectar in response to foliar herbivory on G. hirsutum.
34714845	11	14	theme	adaptive	1835:1842	arg1	mechanism					1844:1852	a potentially adaptive mechanism	1821:1852	a potentially adaptive mechanism which enhances ant attraction to the plant	1821:1895	Further research is needed to determine whether an herbivore-induced increase in sucrose content is typical of Gossypium spp., and whether it constitutes a corollary of systemic sucrose induction, or a potentially adaptive mechanism which enhances ant attraction to the plant.
34714845	11	15	theme	induction	1807:1815	arg1	mechanism					1844:1852	a potentially adaptive mechanism	1821:1852	a potentially adaptive mechanism which enhances ant attraction to the plant	1821:1895	Further research is needed to determine whether an herbivore-induced increase in sucrose content is typical of Gossypium spp., and whether it constitutes a corollary of systemic sucrose induction, or a potentially adaptive mechanism which enhances ant attraction to the plant.
34714845	11	15	theme	induction	1807:1815	arg1	corollary					1777:1785	a corollary	1775:1785	a corollary of systemic sucrose induction	1775:1815	Further research is needed to determine whether an herbivore-induced increase in sucrose content is typical of Gossypium spp., and whether it constitutes a corollary of systemic sucrose induction, or a potentially adaptive mechanism which enhances ant attraction to the plant.
34714845	3	16	theme	nectar	467:472	arg1	production					474:483	EF nectar production	464:483	EF nectar production	464:483	Some EF nectar-bearing plants respond to herbivory by increasing EF nectar production.
34714845	6	17	theme	sucrose	871:877	arg1	content					879:885	the sucrose content	867:885	the sucrose content of bracteal EF nectar	867:907	We found that foliar herbivory significantly increased the sucrose content of bracteal EF nectar while glucose and fructose remained unchanged.
34714845	7	18	theme	caloric	1083:1089	arg1	content					1091:1097	EF nectar caloric content	1073:1097	EF nectar caloric content	1073:1097	Sucrose content is known to influence ant foraging behavior and previous studies of an herbivore-induced increase to EF nectar caloric content found that it led to increased ant activity on the plant.
34714845	9	19	theme	G.	1414:1415	arg1	nectar					1429:1434	G. hirsutum EF nectar	1414:1434	G. hirsutum EF nectar	1414:1434	The ants did not exhibit any preference for either solution, potentially because sucrose is a minor carbohydrate component in G. hirsutum EF nectar: total sugar content was not significantly affected by the increase in sucrose.
34714845	8	20	theme	EF	1212:1213	arg1	solutions					1222:1230	mock EF nectar solutions	1207:1230	mock EF nectar solutions that varied in sucrose content	1207:1261	As a follow-up to our finding, ant recruitment to mock EF nectar solutions that varied in sucrose content was tested in the field.
34714845	10	21	theme	nectar	1590:1595	arg1	responses					1597:1605	cotton's inducible EF nectar responses	1568:1605	cotton's inducible EF nectar responses to herbivory	1568:1618	Nonetheless, our findings raise new questions about cotton's inducible EF nectar responses to herbivory.
34714845	5	22	theme	carbohydrate	717:728	arg1	composition					730:740	the carbohydrate composition	713:740	the carbohydrate composition of bracteal EF nectar	713:762	This study is the first to test for systemically induced changes to the carbohydrate composition of bracteal EF nectar in response to foliar herbivory on G. hirsutum.
34714845	7	23	theme	ant	1130:1132	arg1	activity					1134:1141	increased ant activity	1120:1141	increased ant activity on the plant	1120:1154	Sucrose content is known to influence ant foraging behavior and previous studies of an herbivore-induced increase to EF nectar caloric content found that it led to increased ant activity on the plant.
34714845	8	24	theme	sucrose	1247:1253	arg1	content					1255:1261	sucrose content	1247:1261	sucrose content	1247:1261	As a follow-up to our finding, ant recruitment to mock EF nectar solutions that varied in sucrose content was tested in the field.
34714845	1	25	dep	Gossypium	118:126	arg1	L.					137:138	Gossypium hirsutum L.	118:138	Gossypium hirsutum L.	118:138	Cultivated cotton, such as Gossypium hirsutum L., produces extrafloral (EF) nectar on leaves (foliar) and reproductive structures (bracteal) as an indirect anti-herbivore defense.
34714845	9	26	theme	minor	1382:1386	arg1	component					1401:1409	a minor carbohydrate component	1380:1409	a minor carbohydrate component in G. hirsutum EF nectar	1380:1434	The ants did not exhibit any preference for either solution, potentially because sucrose is a minor carbohydrate component in G. hirsutum EF nectar: total sugar content was not significantly affected by the increase in sucrose.
34714845	9	26	theme	minor	1382:1386	arg1	sucrose					1369:1375	sucrose	1369:1375	sucrose	1369:1375	The ants did not exhibit any preference for either solution, potentially because sucrose is a minor carbohydrate component in G. hirsutum EF nectar: total sugar content was not significantly affected by the increase in sucrose.
34714845	5	27	theme	foliar	779:784	arg1	herbivory					786:794	foliar herbivory	779:794	foliar herbivory on G. hirsutum	779:809	This study is the first to test for systemically induced changes to the carbohydrate composition of bracteal EF nectar in response to foliar herbivory on G. hirsutum.
34714845	5	28	theme	EF	754:755	arg1	nectar					757:762	bracteal EF nectar	745:762	bracteal EF nectar	745:762	This study is the first to test for systemically induced changes to the carbohydrate composition of bracteal EF nectar in response to foliar herbivory on G. hirsutum.
34714845	7	29	theme	EF	1073:1074	arg1	content					1091:1097	EF nectar caloric content	1073:1097	EF nectar caloric content	1073:1097	Sucrose content is known to influence ant foraging behavior and previous studies of an herbivore-induced increase to EF nectar caloric content found that it led to increased ant activity on the plant.
34714845	1	30	theme	Cultivated	91:100	arg1	cotton					102:107	Cultivated cotton	91:107	Cultivated cotton	91:107	Cultivated cotton, such as Gossypium hirsutum L., produces extrafloral (EF) nectar on leaves (foliar) and reproductive structures (bracteal) as an indirect anti-herbivore defense.
34714845	3	31	theme	EF	404:405	arg1	plants					422:427	Some EF nectar-bearing plants	399:427	Some EF nectar-bearing plants	399:427	Some EF nectar-bearing plants respond to herbivory by increasing EF nectar production.
34714845	0	32	theme	Foliar	0:5	arg1	herbivory					7:15	Foliar herbivory	0:15	Foliar herbivory	0:15	Foliar herbivory increases sucrose concentration in bracteal extrafloral nectar of cotton.
34714845	1	33	theme	indirect	238:245	arg1	defense					262:268	an indirect anti-herbivore defense	235:268	an indirect anti-herbivore defense	235:268	Cultivated cotton, such as Gossypium hirsutum L., produces extrafloral (EF) nectar on leaves (foliar) and reproductive structures (bracteal) as an indirect anti-herbivore defense.
34714845	1	33	theme	indirect	238:245	arg1	nectar					167:172	extrafloral (EF) nectar	150:172	extrafloral (EF) nectar on leaves (foliar) and reproductive structures (bracteal)	150:230	Cultivated cotton, such as Gossypium hirsutum L., produces extrafloral (EF) nectar on leaves (foliar) and reproductive structures (bracteal) as an indirect anti-herbivore defense.
34714845	7	34	theme	nectar	1076:1081	arg1	content					1091:1097	EF nectar caloric content	1073:1097	EF nectar caloric content	1073:1097	Sucrose content is known to influence ant foraging behavior and previous studies of an herbivore-induced increase to EF nectar caloric content found that it led to increased ant activity on the plant.
34714845	3	35	theme	nectar-bearing	407:420	arg1	plants					422:427	Some EF nectar-bearing plants	399:427	Some EF nectar-bearing plants	399:427	Some EF nectar-bearing plants respond to herbivory by increasing EF nectar production.
34714845	0	36	theme	sucrose	27:33	arg1	concentration					35:47	sucrose concentration	27:47	sucrose concentration	27:47	Foliar herbivory increases sucrose concentration in bracteal extrafloral nectar of cotton.
34714845	1	37	theme	anti-herbivore	247:260	arg1	defense					262:268	an indirect anti-herbivore defense	235:268	an indirect anti-herbivore defense	235:268	Cultivated cotton, such as Gossypium hirsutum L., produces extrafloral (EF) nectar on leaves (foliar) and reproductive structures (bracteal) as an indirect anti-herbivore defense.
34714845	1	37	theme	anti-herbivore	247:260	arg1	nectar					167:172	extrafloral (EF) nectar	150:172	extrafloral (EF) nectar on leaves (foliar) and reproductive structures (bracteal)	150:230	Cultivated cotton, such as Gossypium hirsutum L., produces extrafloral (EF) nectar on leaves (foliar) and reproductive structures (bracteal) as an indirect anti-herbivore defense.
34714845	7	38	theme	increase	1061:1068	arg1	studies					1029:1035	previous studies	1020:1035	previous studies of an herbivore-induced increase to EF nectar caloric content	1020:1097	Sucrose content is known to influence ant foraging behavior and previous studies of an herbivore-induced increase to EF nectar caloric content found that it led to increased ant activity on the plant.
34714845	10	39	theme	inducible	1577:1585	arg1	responses					1597:1605	cotton's inducible EF nectar responses	1568:1605	cotton's inducible EF nectar responses to herbivory	1568:1618	Nonetheless, our findings raise new questions about cotton's inducible EF nectar responses to herbivory.
34714845	9	40	theme	carbohydrate	1388:1399	arg1	component					1401:1409	a minor carbohydrate component	1380:1409	a minor carbohydrate component in G. hirsutum EF nectar	1380:1434	The ants did not exhibit any preference for either solution, potentially because sucrose is a minor carbohydrate component in G. hirsutum EF nectar: total sugar content was not significantly affected by the increase in sucrose.
34714845	9	40	theme	carbohydrate	1388:1399	arg1	sucrose					1369:1375	sucrose	1369:1375	sucrose	1369:1375	The ants did not exhibit any preference for either solution, potentially because sucrose is a minor carbohydrate component in G. hirsutum EF nectar: total sugar content was not significantly affected by the increase in sucrose.
34714845	11	41	theme	sucrose	1702:1708	arg1	content					1710:1716	sucrose content	1702:1716	sucrose content	1702:1716	Further research is needed to determine whether an herbivore-induced increase in sucrose content is typical of Gossypium spp., and whether it constitutes a corollary of systemic sucrose induction, or a potentially adaptive mechanism which enhances ant attraction to the plant.
34714845	4	42	theme	EF	562:563	arg1	nectar					565:570	foliar EF nectar	555:570	foliar EF nectar	555:570	For instance, herbivore-free G. hirsutum produces more bracteal than foliar EF nectar, but increases its foliar EF nectar production in response to herbivory.
34714845	9	43	theme	total	1437:1441	arg1	content					1449:1455	total sugar content	1437:1455	total sugar content	1437:1455	The ants did not exhibit any preference for either solution, potentially because sucrose is a minor carbohydrate component in G. hirsutum EF nectar: total sugar content was not significantly affected by the increase in sucrose.
34714845	8	44	theme	nectar	1215:1220	arg1	solutions					1222:1230	mock EF nectar solutions	1207:1230	mock EF nectar solutions that varied in sucrose content	1207:1261	As a follow-up to our finding, ant recruitment to mock EF nectar solutions that varied in sucrose content was tested in the field.
34714845	5	45	theme	nectar	757:762	arg1	composition					730:740	the carbohydrate composition	713:740	the carbohydrate composition of bracteal EF nectar	713:762	This study is the first to test for systemically induced changes to the carbohydrate composition of bracteal EF nectar in response to foliar herbivory on G. hirsutum.
34714845	4	46	theme	foliar	555:560	arg1	nectar					565:570	foliar EF nectar	555:570	foliar EF nectar	555:570	For instance, herbivore-free G. hirsutum produces more bracteal than foliar EF nectar, but increases its foliar EF nectar production in response to herbivory.
34714845	11	47	theme	systemic	1790:1797	arg1	induction					1807:1815	systemic sucrose induction	1790:1815	systemic sucrose induction	1790:1815	Further research is needed to determine whether an herbivore-induced increase in sucrose content is typical of Gossypium spp., and whether it constitutes a corollary of systemic sucrose induction, or a potentially adaptive mechanism which enhances ant attraction to the plant.
34714845	4	48	from	production	608:617	arg1	response					622:629	response	622:629	response to herbivory	622:642	For instance, herbivore-free G. hirsutum produces more bracteal than foliar EF nectar, but increases its foliar EF nectar production in response to herbivory.
34714845	9	49	theme	hirsutum	1417:1424	arg1	nectar					1429:1434	G. hirsutum EF nectar	1414:1434	G. hirsutum EF nectar	1414:1434	The ants did not exhibit any preference for either solution, potentially because sucrose is a minor carbohydrate component in G. hirsutum EF nectar: total sugar content was not significantly affected by the increase in sucrose.
34714845	5	50	theme	induced	694:700	arg1	changes					702:708	systemically induced changes	681:708	systemically induced changes to the carbohydrate composition of bracteal EF nectar	681:762	This study is the first to test for systemically induced changes to the carbohydrate composition of bracteal EF nectar in response to foliar herbivory on G. hirsutum.
34714845	6	51	theme	foliar	826:831	arg1	herbivory					833:841	foliar herbivory	826:841	foliar herbivory	826:841	We found that foliar herbivory significantly increased the sucrose content of bracteal EF nectar while glucose and fructose remained unchanged.
34714845	11	52	theme	herbivore-induced	1672:1688	arg1	typical					1721:1727	typical	1721:1727	typical	1721:1727	Further research is needed to determine whether an herbivore-induced increase in sucrose content is typical of Gossypium spp., and whether it constitutes a corollary of systemic sucrose induction, or a potentially adaptive mechanism which enhances ant attraction to the plant.
34714845	11	52	theme	herbivore-induced	1672:1688	arg1	increase					1690:1697	an herbivore-induced increase	1669:1697	an herbivore-induced increase in sucrose content	1669:1716	Further research is needed to determine whether an herbivore-induced increase in sucrose content is typical of Gossypium spp., and whether it constitutes a corollary of systemic sucrose induction, or a potentially adaptive mechanism which enhances ant attraction to the plant.
34714845	7	53	theme	foraging	998:1005	arg1	behavior					1007:1014	ant foraging behavior	994:1014	ant foraging behavior	994:1014	Sucrose content is known to influence ant foraging behavior and previous studies of an herbivore-induced increase to EF nectar caloric content found that it led to increased ant activity on the plant.
34714845	2	54	theme	herbivorous	378:388	arg1	insects					390:396	herbivorous insects	378:396	herbivorous insects	378:396	In exchange for this carbohydrate-rich substance, predatory insects such as ants protect the plant against herbivorous insects.
34714845	0	55	theme	extrafloral	61:71	arg1	nectar					73:78	bracteal extrafloral nectar	52:78	bracteal extrafloral nectar of cotton	52:88	Foliar herbivory increases sucrose concentration in bracteal extrafloral nectar of cotton.
34714845	8	56	theme	mock	1207:1210	arg1	solutions					1222:1230	mock EF nectar solutions	1207:1230	mock EF nectar solutions that varied in sucrose content	1207:1261	As a follow-up to our finding, ant recruitment to mock EF nectar solutions that varied in sucrose content was tested in the field.
34714845	2	57	theme	predatory	321:329	arg1	insects					331:337	predatory insects	321:337	predatory insects such as ants	321:350	In exchange for this carbohydrate-rich substance, predatory insects such as ants protect the plant against herbivorous insects.
34714845	2	57	theme	predatory	321:329	arg1	ants					347:350	ants	347:350	ants	347:350	In exchange for this carbohydrate-rich substance, predatory insects such as ants protect the plant against herbivorous insects.
34714845	0	58	theme	bracteal	52:59	arg1	nectar					73:78	bracteal extrafloral nectar	52:78	bracteal extrafloral nectar of cotton	52:88	Foliar herbivory increases sucrose concentration in bracteal extrafloral nectar of cotton.
34714845	11	59	from	increase	1690:1697	arg1	content					1710:1716	sucrose content	1702:1716	sucrose content	1702:1716	Further research is needed to determine whether an herbivore-induced increase in sucrose content is typical of Gossypium spp., and whether it constitutes a corollary of systemic sucrose induction, or a potentially adaptive mechanism which enhances ant attraction to the plant.
34714845	11	60	theme	sucrose	1799:1805	arg1	induction					1807:1815	systemic sucrose induction	1790:1815	systemic sucrose induction	1790:1815	Further research is needed to determine whether an herbivore-induced increase in sucrose content is typical of Gossypium spp., and whether it constitutes a corollary of systemic sucrose induction, or a potentially adaptive mechanism which enhances ant attraction to the plant.
34714845	11	61	theme	Further	1621:1627	arg1	research					1629:1636	Further research	1621:1636	Further research	1621:1636	Further research is needed to determine whether an herbivore-induced increase in sucrose content is typical of Gossypium spp., and whether it constitutes a corollary of systemic sucrose induction, or a potentially adaptive mechanism which enhances ant attraction to the plant.
34714845	7	62	theme	increased	1120:1128	arg1	activity					1134:1141	increased ant activity	1120:1141	increased ant activity on the plant	1120:1154	Sucrose content is known to influence ant foraging behavior and previous studies of an herbivore-induced increase to EF nectar caloric content found that it led to increased ant activity on the plant.
34714845	7	63	theme	ant	994:996	arg1	behavior					1007:1014	ant foraging behavior	994:1014	ant foraging behavior	994:1014	Sucrose content is known to influence ant foraging behavior and previous studies of an herbivore-induced increase to EF nectar caloric content found that it led to increased ant activity on the plant.
34714845	3	64	theme	EF	464:465	arg1	production					474:483	EF nectar production	464:483	EF nectar production	464:483	Some EF nectar-bearing plants respond to herbivory by increasing EF nectar production.
34714845	4	65	theme	foliar	591:596	arg1	production					608:617	its foliar EF nectar production	587:617	its foliar EF nectar production in response to herbivory	587:642	For instance, herbivore-free G. hirsutum produces more bracteal than foliar EF nectar, but increases its foliar EF nectar production in response to herbivory.
34714845	7	66	theme	previous	1020:1027	arg1	studies					1029:1035	previous studies	1020:1035	previous studies of an herbivore-induced increase to EF nectar caloric content	1020:1097	Sucrose content is known to influence ant foraging behavior and previous studies of an herbivore-induced increase to EF nectar caloric content found that it led to increased ant activity on the plant.
34714845	1	67	from	nectar	167:172	arg1	structures					210:219	reproductive structures	197:219	reproductive structures (bracteal)	197:230	Cultivated cotton, such as Gossypium hirsutum L., produces extrafloral (EF) nectar on leaves (foliar) and reproductive structures (bracteal) as an indirect anti-herbivore defense.
34714845	1	67	from	nectar	167:172	arg1	foliar					185:190	foliar	185:190	foliar	185:190	Cultivated cotton, such as Gossypium hirsutum L., produces extrafloral (EF) nectar on leaves (foliar) and reproductive structures (bracteal) as an indirect anti-herbivore defense.
34714845	1	67	from	nectar	167:172	arg1	bracteal					222:229	bracteal	222:229	bracteal	222:229	Cultivated cotton, such as Gossypium hirsutum L., produces extrafloral (EF) nectar on leaves (foliar) and reproductive structures (bracteal) as an indirect anti-herbivore defense.
34714845	1	67	from	nectar	167:172	arg1	leaves					177:182	leaves	177:182	leaves	177:182	Cultivated cotton, such as Gossypium hirsutum L., produces extrafloral (EF) nectar on leaves (foliar) and reproductive structures (bracteal) as an indirect anti-herbivore defense.
34714845	2	68	theme	carbohydrate-rich	292:308	arg1	substance					310:318	this carbohydrate-rich substance	287:318	this carbohydrate-rich substance	287:318	In exchange for this carbohydrate-rich substance, predatory insects such as ants protect the plant against herbivorous insects.
34714845	1	69	theme	reproductive	197:208	arg1	structures					210:219	reproductive structures	197:219	reproductive structures (bracteal)	197:230	Cultivated cotton, such as Gossypium hirsutum L., produces extrafloral (EF) nectar on leaves (foliar) and reproductive structures (bracteal) as an indirect anti-herbivore defense.
34714845	1	69	theme	reproductive	197:208	arg1	bracteal					222:229	bracteal	222:229	bracteal	222:229	Cultivated cotton, such as Gossypium hirsutum L., produces extrafloral (EF) nectar on leaves (foliar) and reproductive structures (bracteal) as an indirect anti-herbivore defense.
34714845	9	70	theme	EF	1426:1427	arg1	nectar					1429:1434	G. hirsutum EF nectar	1414:1434	G. hirsutum EF nectar	1414:1434	The ants did not exhibit any preference for either solution, potentially because sucrose is a minor carbohydrate component in G. hirsutum EF nectar: total sugar content was not significantly affected by the increase in sucrose.
34714845	9	71	from	component	1401:1409	arg1	nectar					1429:1434	G. hirsutum EF nectar	1414:1434	G. hirsutum EF nectar	1414:1434	The ants did not exhibit any preference for either solution, potentially because sucrose is a minor carbohydrate component in G. hirsutum EF nectar: total sugar content was not significantly affected by the increase in sucrose.
34714845	11	72	theme	ant	1869:1871	arg1	attraction					1873:1882	ant attraction	1869:1882	ant attraction to the plant	1869:1895	Further research is needed to determine whether an herbivore-induced increase in sucrose content is typical of Gossypium spp., and whether it constitutes a corollary of systemic sucrose induction, or a potentially adaptive mechanism which enhances ant attraction to the plant.
34714845	10	73	theme	EF	1587:1588	arg1	responses					1597:1605	cotton's inducible EF nectar responses	1568:1605	cotton's inducible EF nectar responses to herbivory	1568:1618	Nonetheless, our findings raise new questions about cotton's inducible EF nectar responses to herbivory.
34714845	4	74	theme	nectar	601:606	arg1	production					608:617	its foliar EF nectar production	587:617	its foliar EF nectar production in response to herbivory	587:642	For instance, herbivore-free G. hirsutum produces more bracteal than foliar EF nectar, but increases its foliar EF nectar production in response to herbivory.
34714845	8	75	theme	ant	1188:1190	arg1	follow-up					1162:1170	a follow-up	1160:1170	a follow-up to our finding	1160:1185	As a follow-up to our finding, ant recruitment to mock EF nectar solutions that varied in sucrose content was tested in the field.
34714845	8	75	theme	ant	1188:1190	arg1	recruitment					1192:1202	ant recruitment	1188:1202	ant recruitment to mock EF nectar solutions that varied in sucrose content	1188:1261	As a follow-up to our finding, ant recruitment to mock EF nectar solutions that varied in sucrose content was tested in the field.
34714845	6	76	theme	nectar	902:907	arg1	content					879:885	the sucrose content	867:885	the sucrose content of bracteal EF nectar	867:907	We found that foliar herbivory significantly increased the sucrose content of bracteal EF nectar while glucose and fructose remained unchanged.
34714845	4	77	theme	EF	598:599	arg1	production					608:617	its foliar EF nectar production	587:617	its foliar EF nectar production in response to herbivory	587:642	For instance, herbivore-free G. hirsutum produces more bracteal than foliar EF nectar, but increases its foliar EF nectar production in response to herbivory.
33973306	4	0	theme	chemical	939:946	arg1	shift					948:952	a chemical shift	937:952	a chemical shift from saturated to unsaturated lipids	937:989	Fatty acid methyl ester analysis showed a chemical shift from saturated to unsaturated lipids with WfE supplementation.
33973306	3	1	theme	based	589:593	arg1	studies					607:613	1 H-NMR based metabolomic studies	581:613	1 H-NMR based metabolomic studies	581:613	1 H-NMR based metabolomic studies indicated higher glucose : lactate and glucose : acetate in the extracellular matrix with significant variation (P < 0·05) in intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition.
33973306	4	2	theme	acid	903:906	arg1	ester					915:919	Fatty acid methyl ester	897:919	Fatty acid methyl ester analysis	897:928	Fatty acid methyl ester analysis showed a chemical shift from saturated to unsaturated lipids with WfE supplementation.
33973306	2	3	from	0·5	387:389	arg1	supplementation					368:382	WfE supplementation	364:382	WfE supplementation at 0·5 and 1 mg ml-1	364:403	METHODS AND RESULTS WfE supplementation at 0·5 and 1 mg ml-1 stimulated probiotic growth (P < 0·05), enhanced adhesion to CaCO2 cells (P < 0·05) while inhibiting foodborne pathogens Escherichia coli and Staphylococcus aureus (P < 0·05).
33973306	4	4	theme	ester	915:919	arg1	analysis					921:928	Fatty acid methyl ester analysis	897:928	Fatty acid methyl ester analysis	897:928	Fatty acid methyl ester analysis showed a chemical shift from saturated to unsaturated lipids with WfE supplementation.
33973306	2	5	from	1 mg ml-1	395:403	arg1	supplementation					368:382	WfE supplementation	364:382	WfE supplementation at 0·5 and 1 mg ml-1	364:403	METHODS AND RESULTS WfE supplementation at 0·5 and 1 mg ml-1 stimulated probiotic growth (P < 0·05), enhanced adhesion to CaCO2 cells (P < 0·05) while inhibiting foodborne pathogens Escherichia coli and Staphylococcus aureus (P < 0·05).
33973306	3	6	theme	higher	625:630	arg1	glucose 					632:639	higher glucose 	625:639	higher glucose 	625:639	1 H-NMR based metabolomic studies indicated higher glucose : lactate and glucose : acetate in the extracellular matrix with significant variation (P < 0·05) in intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition.
33973306	1	7	theme	Woodfordia	223:232	arg1	extract					244:250	Woodfordia fruticosa extract	223:250	Woodfordia fruticosa extract (WfE)	223:256	AIM To investigate the effect of Woodfordia fruticosa extract (WfE) on two probiotic bacteria: Lacticaseibacillus casei and Lacticaseibacillus rhamnosus.
33973306	1	7	theme	Woodfordia	223:232	arg1	WfE					253:255	WfE	253:255	WfE	253:255	AIM To investigate the effect of Woodfordia fruticosa extract (WfE) on two probiotic bacteria: Lacticaseibacillus casei and Lacticaseibacillus rhamnosus.
33973306	5	8	from	variation	1092:1100	arg1	response					1124:1131	response	1124:1131	response to WfE supplementation	1124:1154	PCA plots indicated clear discrimination between test groups, highlighting variation in metabolite pool in response to WfE supplementation.
33973306	5	8	from	variation	1092:1100	arg1	pool					1116:1119	metabolite pool	1105:1119	metabolite pool	1105:1119	PCA plots indicated clear discrimination between test groups, highlighting variation in metabolite pool in response to WfE supplementation.
33973306	1	9	theme	extract	244:250	arg1	effect					213:218	the effect	209:218	the effect of Woodfordia fruticosa extract (WfE) on two probiotic bacteria	209:282	AIM To investigate the effect of Woodfordia fruticosa extract (WfE) on two probiotic bacteria: Lacticaseibacillus casei and Lacticaseibacillus rhamnosus.
33973306	4	10	theme	WfE	996:998	arg1	supplementation					1000:1014	WfE supplementation	996:1014	WfE supplementation	996:1014	Fatty acid methyl ester analysis showed a chemical shift from saturated to unsaturated lipids with WfE supplementation.
33973306	0	11	theme	metabolite	173:182	arg1	pool					184:187	adapted intracellular and extracellular metabolite pool	133:187	adapted intracellular and extracellular metabolite pool	133:187	Woodfordia fruticosa extract supplementation stimulates the growth of Lacticaseibacillus casei and Lacticaseibacillus rhamnosus with adapted intracellular and extracellular metabolite pool.
33973306	3	12	theme	amino	847:851	arg1	acids					853:857	branched-chain amino acids	832:857	branched-chain amino acids	832:857	1 H-NMR based metabolomic studies indicated higher glucose : lactate and glucose : acetate in the extracellular matrix with significant variation (P < 0·05) in intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition.
33973306	8	13	theme	phytonutrient-rich	1621:1638	arg1	environment					1644:1654	a phytonutrient-rich WfE environment	1619:1654	a phytonutrient-rich WfE environment	1619:1654	The extended metabolomic studies shed light on the positive interaction between phytonutrients and beneficial bacteria that possibly help them to adapt to a phytonutrient-rich WfE environment.
33973306	3	14	theme	composition	884:894	arg1	concentrations					755:768	intracellular concentrations	741:768	intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition	741:894	1 H-NMR based metabolomic studies indicated higher glucose : lactate and glucose : acetate in the extracellular matrix with significant variation (P < 0·05) in intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition.
33973306	9	15	theme	prebiotic	1676:1684	arg1	attributes					1686:1695	potential prebiotic attributes	1666:1695	potential prebiotic attributes	1666:1695	WfE with potential prebiotic attributes can be used in the development of novel synbiotic functional products targeting gut microbial modulation to improve health.
33973306	9	16	with	WfE	1657:1659	arg1	attributes					1686:1695	potential prebiotic attributes	1666:1695	potential prebiotic attributes	1666:1695	WfE with potential prebiotic attributes can be used in the development of novel synbiotic functional products targeting gut microbial modulation to improve health.
33973306	2	17	dep	stimulated	405:414	arg1	enhanced					445:452	enhanced	445:452	enhanced adhesion to CaCO2 cells (P < 0·05) while inhibiting foodborne pathogens Escherichia coli and Staphylococcus aureus (P < 0·05)	445:578	METHODS AND RESULTS WfE supplementation at 0·5 and 1 mg ml-1 stimulated probiotic growth (P < 0·05), enhanced adhesion to CaCO2 cells (P < 0·05) while inhibiting foodborne pathogens Escherichia coli and Staphylococcus aureus (P < 0·05).
33973306	3	18	theme	cellular	869:876	arg1	composition					884:894	total cellular lipid composition	863:894	total cellular lipid composition	863:894	1 H-NMR based metabolomic studies indicated higher glucose : lactate and glucose : acetate in the extracellular matrix with significant variation (P < 0·05) in intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition.
33973306	0	19	theme	rhamnosus	118:126	arg1	growth					60:65	the growth	56:65	the growth of Lacticaseibacillus casei and Lacticaseibacillus rhamnosus	56:126	Woodfordia fruticosa extract supplementation stimulates the growth of Lacticaseibacillus casei and Lacticaseibacillus rhamnosus with adapted intracellular and extracellular metabolite pool.
33973306	5	20	theme	clear	1037:1041	arg1	discrimination					1043:1056	clear discrimination	1037:1056	clear discrimination between test groups	1037:1076	PCA plots indicated clear discrimination between test groups, highlighting variation in metabolite pool in response to WfE supplementation.
33973306	8	21	theme	metabolomic	1477:1487	arg1	studies					1489:1495	The extended metabolomic studies	1464:1495	The extended metabolomic studies	1464:1495	The extended metabolomic studies shed light on the positive interaction between phytonutrients and beneficial bacteria that possibly help them to adapt to a phytonutrient-rich WfE environment.
33973306	0	22	theme	adapted	133:139	arg1	pool					184:187	adapted intracellular and extracellular metabolite pool	133:187	adapted intracellular and extracellular metabolite pool	133:187	Woodfordia fruticosa extract supplementation stimulates the growth of Lacticaseibacillus casei and Lacticaseibacillus rhamnosus with adapted intracellular and extracellular metabolite pool.
33973306	6	23	theme	CONCLUSION	1157:1166	arg1	WfE					1187:1189	CONCLUSION Phytonutrient-rich WfE	1157:1189	CONCLUSION Phytonutrient-rich WfE	1157:1189	CONCLUSION Phytonutrient-rich WfE exhibited prebiotic-like attributes, and probiotic bacteria showed altered metabolite pools as an adaptive mechanism.
33973306	0	24	theme	casei	89:93	arg1	growth					60:65	the growth	56:65	the growth of Lacticaseibacillus casei and Lacticaseibacillus rhamnosus	56:126	Woodfordia fruticosa extract supplementation stimulates the growth of Lacticaseibacillus casei and Lacticaseibacillus rhamnosus with adapted intracellular and extracellular metabolite pool.
33973306	0	25	theme	Woodfordia	0:9	arg1	supplementation					29:43	Woodfordia fruticosa extract supplementation	0:43	Woodfordia fruticosa extract supplementation	0:43	Woodfordia fruticosa extract supplementation stimulates the growth of Lacticaseibacillus casei and Lacticaseibacillus rhamnosus with adapted intracellular and extracellular metabolite pool.
33973306	7	26	theme	gut	1433:1435	arg1	probiotics					1452:1461	gut representative probiotics	1433:1461	gut representative probiotics	1433:1461	SIGNIFICANCE AND IMPACT OF THE STUDY This is the first report providing insights into the prebiotic-like activity of WfE on gut representative probiotics.
33973306	2	27	theme	CaCO2	466:470	arg1	P < 0·05					479:486	P < 0·05	479:486	P < 0·05	479:486	METHODS AND RESULTS WfE supplementation at 0·5 and 1 mg ml-1 stimulated probiotic growth (P < 0·05), enhanced adhesion to CaCO2 cells (P < 0·05) while inhibiting foodborne pathogens Escherichia coli and Staphylococcus aureus (P < 0·05).
33973306	2	27	theme	CaCO2	466:470	arg1	cells					472:476	CaCO2 cells	466:476	CaCO2 cells (P < 0·05)	466:487	METHODS AND RESULTS WfE supplementation at 0·5 and 1 mg ml-1 stimulated probiotic growth (P < 0·05), enhanced adhesion to CaCO2 cells (P < 0·05) while inhibiting foodborne pathogens Escherichia coli and Staphylococcus aureus (P < 0·05).
33973306	0	28	theme	extract	21:27	arg1	supplementation					29:43	Woodfordia fruticosa extract supplementation	0:43	Woodfordia fruticosa extract supplementation	0:43	Woodfordia fruticosa extract supplementation stimulates the growth of Lacticaseibacillus casei and Lacticaseibacillus rhamnosus with adapted intracellular and extracellular metabolite pool.
33973306	7	29	theme	WfE	1426:1428	arg1	activity					1414:1421	the prebiotic-like activity	1395:1421	the prebiotic-like activity of WfE on gut representative probiotics	1395:1461	SIGNIFICANCE AND IMPACT OF THE STUDY This is the first report providing insights into the prebiotic-like activity of WfE on gut representative probiotics.
33973306	8	30	theme	positive	1515:1522	arg1	interaction					1524:1534	the positive interaction	1511:1534	the positive interaction between phytonutrients and beneficial bacteria that possibly help them to adapt to a phytonutrient-rich WfE environment	1511:1654	The extended metabolomic studies shed light on the positive interaction between phytonutrients and beneficial bacteria that possibly help them to adapt to a phytonutrient-rich WfE environment.
33973306	2	31	theme	foodborne	506:514	arg1	pathogens					516:524	foodborne pathogens Escherichia coli and Staphylococcus aureus (P < 0·05)	506:578	foodborne pathogens Escherichia coli and Staphylococcus aureus (P < 0·05)	506:578	METHODS AND RESULTS WfE supplementation at 0·5 and 1 mg ml-1 stimulated probiotic growth (P < 0·05), enhanced adhesion to CaCO2 cells (P < 0·05) while inhibiting foodborne pathogens Escherichia coli and Staphylococcus aureus (P < 0·05).
33973306	9	32	theme	synbiotic	1737:1745	arg1	products					1758:1765	novel synbiotic functional products	1731:1765	novel synbiotic functional products targeting gut microbial modulation to improve health	1731:1818	WfE with potential prebiotic attributes can be used in the development of novel synbiotic functional products targeting gut microbial modulation to improve health.
33973306	3	33	theme	extracellular	679:691	arg1	matrix					693:698	the extracellular matrix	675:698	the extracellular matrix	675:698	1 H-NMR based metabolomic studies indicated higher glucose : lactate and glucose : acetate in the extracellular matrix with significant variation (P < 0·05) in intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition.
33973306	9	34	theme	products	1758:1765	arg1	development					1716:1726	the development	1712:1726	the development of novel synbiotic functional products targeting gut microbial modulation to improve health	1712:1818	WfE with potential prebiotic attributes can be used in the development of novel synbiotic functional products targeting gut microbial modulation to improve health.
33973306	6	35	theme	probiotic	1232:1240	arg1	bacteria					1242:1249	probiotic bacteria	1232:1249	probiotic bacteria	1232:1249	CONCLUSION Phytonutrient-rich WfE exhibited prebiotic-like attributes, and probiotic bacteria showed altered metabolite pools as an adaptive mechanism.
33973306	9	36	theme	gut	1777:1779	arg1	modulation					1791:1800	gut microbial modulation	1777:1800	gut microbial modulation to improve health	1777:1818	WfE with potential prebiotic attributes can be used in the development of novel synbiotic functional products targeting gut microbial modulation to improve health.
33973306	6	37	theme	prebiotic-like	1201:1214	arg1	attributes					1216:1225	prebiotic-like attributes	1201:1225	prebiotic-like attributes	1201:1225	CONCLUSION Phytonutrient-rich WfE exhibited prebiotic-like attributes, and probiotic bacteria showed altered metabolite pools as an adaptive mechanism.
33973306	9	38	theme	functional	1747:1756	arg1	products					1758:1765	novel synbiotic functional products	1731:1765	novel synbiotic functional products targeting gut microbial modulation to improve health	1731:1818	WfE with potential prebiotic attributes can be used in the development of novel synbiotic functional products targeting gut microbial modulation to improve health.
33973306	3	39	theme	H-NMR	583:587	arg1	studies					607:613	1 H-NMR based metabolomic studies	581:613	1 H-NMR based metabolomic studies	581:613	1 H-NMR based metabolomic studies indicated higher glucose : lactate and glucose : acetate in the extracellular matrix with significant variation (P < 0·05) in intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition.
33973306	6	40	theme	adaptive	1289:1296	arg1	pools					1277:1281	metabolite pools	1266:1281	metabolite pools	1266:1281	CONCLUSION Phytonutrient-rich WfE exhibited prebiotic-like attributes, and probiotic bacteria showed altered metabolite pools as an adaptive mechanism.
33973306	6	40	theme	adaptive	1289:1296	arg1	mechanism					1298:1306	an adaptive mechanism	1286:1306	an adaptive mechanism	1286:1306	CONCLUSION Phytonutrient-rich WfE exhibited prebiotic-like attributes, and probiotic bacteria showed altered metabolite pools as an adaptive mechanism.
33973306	3	41	theme	acetate	782:788	arg1	concentrations					755:768	intracellular concentrations	741:768	intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition	741:894	1 H-NMR based metabolomic studies indicated higher glucose : lactate and glucose : acetate in the extracellular matrix with significant variation (P < 0·05) in intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition.
33973306	4	42	theme	Fatty	897:901	arg1	ester					915:919	Fatty acid methyl ester	897:919	Fatty acid methyl ester analysis	897:928	Fatty acid methyl ester analysis showed a chemical shift from saturated to unsaturated lipids with WfE supplementation.
33973306	9	43	used	used	1704:1707	arg2	WfE					1657:1659	WfE	1657:1659	WfE with potential prebiotic attributes	1657:1695	WfE with potential prebiotic attributes can be used in the development of novel synbiotic functional products targeting gut microbial modulation to improve health.
33973306	6	44	dep	showed	1251:1256	arg1	altered					1258:1264	altered	1258:1264	showed altered metabolite pools as an adaptive mechanism	1251:1306	CONCLUSION Phytonutrient-rich WfE exhibited prebiotic-like attributes, and probiotic bacteria showed altered metabolite pools as an adaptive mechanism.
33973306	3	45	theme	lactate	773:779	arg1	concentrations					755:768	intracellular concentrations	741:768	intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition	741:894	1 H-NMR based metabolomic studies indicated higher glucose : lactate and glucose : acetate in the extracellular matrix with significant variation (P < 0·05) in intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition.
33973306	2	46	theme	WfE	364:366	arg1	supplementation					368:382	WfE supplementation	364:382	WfE supplementation at 0·5 and 1 mg ml-1	364:403	METHODS AND RESULTS WfE supplementation at 0·5 and 1 mg ml-1 stimulated probiotic growth (P < 0·05), enhanced adhesion to CaCO2 cells (P < 0·05) while inhibiting foodborne pathogens Escherichia coli and Staphylococcus aureus (P < 0·05).
33973306	4	47	theme	methyl	908:913	arg1	ester					915:919	Fatty acid methyl ester	897:919	Fatty acid methyl ester analysis	897:928	Fatty acid methyl ester analysis showed a chemical shift from saturated to unsaturated lipids with WfE supplementation.
33973306	6	48	theme	metabolite	1266:1275	arg1	pools					1277:1281	metabolite pools	1266:1281	metabolite pools	1266:1281	CONCLUSION Phytonutrient-rich WfE exhibited prebiotic-like attributes, and probiotic bacteria showed altered metabolite pools as an adaptive mechanism.
33973306	6	48	theme	metabolite	1266:1275	arg1	mechanism					1298:1306	an adaptive mechanism	1286:1306	an adaptive mechanism	1286:1306	CONCLUSION Phytonutrient-rich WfE exhibited prebiotic-like attributes, and probiotic bacteria showed altered metabolite pools as an adaptive mechanism.
33973306	3	49	theme	dimethylamine	802:814	arg1	concentrations					755:768	intracellular concentrations	741:768	intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition	741:894	1 H-NMR based metabolomic studies indicated higher glucose : lactate and glucose : acetate in the extracellular matrix with significant variation (P < 0·05) in intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition.
33973306	0	50	theme	extracellular	159:171	arg1	pool					184:187	adapted intracellular and extracellular metabolite pool	133:187	adapted intracellular and extracellular metabolite pool	133:187	Woodfordia fruticosa extract supplementation stimulates the growth of Lacticaseibacillus casei and Lacticaseibacillus rhamnosus with adapted intracellular and extracellular metabolite pool.
33973306	3	51	theme	metabolomic	595:605	arg1	studies					607:613	1 H-NMR based metabolomic studies	581:613	1 H-NMR based metabolomic studies	581:613	1 H-NMR based metabolomic studies indicated higher glucose : lactate and glucose : acetate in the extracellular matrix with significant variation (P < 0·05) in intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition.
33973306	1	52	theme	fruticosa	234:242	arg1	extract					244:250	Woodfordia fruticosa extract	223:250	Woodfordia fruticosa extract (WfE)	223:256	AIM To investigate the effect of Woodfordia fruticosa extract (WfE) on two probiotic bacteria: Lacticaseibacillus casei and Lacticaseibacillus rhamnosus.
33973306	1	52	theme	fruticosa	234:242	arg1	WfE					253:255	WfE	253:255	WfE	253:255	AIM To investigate the effect of Woodfordia fruticosa extract (WfE) on two probiotic bacteria: Lacticaseibacillus casei and Lacticaseibacillus rhamnosus.
33973306	4	53	theme	unsaturated	972:982	arg1	lipids					984:989	unsaturated lipids	972:989	unsaturated lipids	972:989	Fatty acid methyl ester analysis showed a chemical shift from saturated to unsaturated lipids with WfE supplementation.
33973306	3	54	theme	glutamate	791:799	arg1	concentrations					755:768	intracellular concentrations	741:768	intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition	741:894	1 H-NMR based metabolomic studies indicated higher glucose : lactate and glucose : acetate in the extracellular matrix with significant variation (P < 0·05) in intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition.
33973306	3	55	theme	branched-chain	832:845	arg1	acids					853:857	branched-chain amino acids	832:857	branched-chain amino acids	832:857	1 H-NMR based metabolomic studies indicated higher glucose : lactate and glucose : acetate in the extracellular matrix with significant variation (P < 0·05) in intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition.
33973306	2	56	dep	METHODS	344:350	arg1	stimulated					405:414	stimulated	405:414	stimulated probiotic growth (P < 0·05)	405:442	METHODS AND RESULTS WfE supplementation at 0·5 and 1 mg ml-1 stimulated probiotic growth (P < 0·05), enhanced adhesion to CaCO2 cells (P < 0·05) while inhibiting foodborne pathogens Escherichia coli and Staphylococcus aureus (P < 0·05).
33973306	3	57	theme	phenylalanine	817:829	arg1	concentrations					755:768	intracellular concentrations	741:768	intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition	741:894	1 H-NMR based metabolomic studies indicated higher glucose : lactate and glucose : acetate in the extracellular matrix with significant variation (P < 0·05) in intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition.
33973306	4	58	from	saturated	959:967	arg1	shift					948:952	a chemical shift	937:952	a chemical shift from saturated to unsaturated lipids	937:989	Fatty acid methyl ester analysis showed a chemical shift from saturated to unsaturated lipids with WfE supplementation.
33973306	3	59	theme	total	863:867	arg1	composition					884:894	total cellular lipid composition	863:894	total cellular lipid composition	863:894	1 H-NMR based metabolomic studies indicated higher glucose : lactate and glucose : acetate in the extracellular matrix with significant variation (P < 0·05) in intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition.
33973306	7	60	theme	STUDY	1340:1344	arg1	IMPACT					1326:1331	IMPACT	1326:1331	IMPACT	1326:1331	SIGNIFICANCE AND IMPACT OF THE STUDY This is the first report providing insights into the prebiotic-like activity of WfE on gut representative probiotics.
33973306	7	60	theme	STUDY	1340:1344	arg1	report					1364:1369	the first report	1354:1369	the first report providing insights into the prebiotic-like activity of WfE on gut representative probiotics	1354:1461	SIGNIFICANCE AND IMPACT OF THE STUDY This is the first report providing insights into the prebiotic-like activity of WfE on gut representative probiotics.
33973306	7	60	theme	STUDY	1340:1344	arg1	SIGNIFICANCE					1309:1320	SIGNIFICANCE	1309:1320	SIGNIFICANCE	1309:1320	SIGNIFICANCE AND IMPACT OF THE STUDY This is the first report providing insights into the prebiotic-like activity of WfE on gut representative probiotics.
33973306	5	61	theme	WfE	1136:1138	arg1	supplementation					1140:1154	WfE supplementation	1136:1154	WfE supplementation	1136:1154	PCA plots indicated clear discrimination between test groups, highlighting variation in metabolite pool in response to WfE supplementation.
33973306	8	62	theme	WfE	1640:1642	arg1	environment					1644:1654	a phytonutrient-rich WfE environment	1619:1654	a phytonutrient-rich WfE environment	1619:1654	The extended metabolomic studies shed light on the positive interaction between phytonutrients and beneficial bacteria that possibly help them to adapt to a phytonutrient-rich WfE environment.
33973306	3	63	theme	acids	853:857	arg1	concentrations					755:768	intracellular concentrations	741:768	intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition	741:894	1 H-NMR based metabolomic studies indicated higher glucose : lactate and glucose : acetate in the extracellular matrix with significant variation (P < 0·05) in intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition.
33973306	3	64	with	 acetate	663:670	arg1	variation					717:725	significant variation	705:725	significant variation (P < 0·05) in intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition	705:894	1 H-NMR based metabolomic studies indicated higher glucose : lactate and glucose : acetate in the extracellular matrix with significant variation (P < 0·05) in intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition.
33973306	3	64	with	 acetate	663:670	arg1	P < 0·05					728:735	P < 0·05	728:735	P < 0·05	728:735	1 H-NMR based metabolomic studies indicated higher glucose : lactate and glucose : acetate in the extracellular matrix with significant variation (P < 0·05) in intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition.
33973306	1	65	from	effect	213:218	arg1	bacteria					275:282	two probiotic bacteria	261:282	two probiotic bacteria	261:282	AIM To investigate the effect of Woodfordia fruticosa extract (WfE) on two probiotic bacteria: Lacticaseibacillus casei and Lacticaseibacillus rhamnosus.
33973306	9	66	theme	potential	1666:1674	arg1	attributes					1686:1695	potential prebiotic attributes	1666:1695	potential prebiotic attributes	1666:1695	WfE with potential prebiotic attributes can be used in the development of novel synbiotic functional products targeting gut microbial modulation to improve health.
33973306	2	67	dep	pathogens	516:524	arg1	P < 0·05					570:577	P < 0·05	570:577	P < 0·05	570:577	METHODS AND RESULTS WfE supplementation at 0·5 and 1 mg ml-1 stimulated probiotic growth (P < 0·05), enhanced adhesion to CaCO2 cells (P < 0·05) while inhibiting foodborne pathogens Escherichia coli and Staphylococcus aureus (P < 0·05).
33973306	2	67	dep	pathogens	516:524	arg1	coli					538:541	Escherichia coli	526:541	Escherichia coli	526:541	METHODS AND RESULTS WfE supplementation at 0·5 and 1 mg ml-1 stimulated probiotic growth (P < 0·05), enhanced adhesion to CaCO2 cells (P < 0·05) while inhibiting foodborne pathogens Escherichia coli and Staphylococcus aureus (P < 0·05).
33973306	2	67	dep	pathogens	516:524	arg1	aureus					562:567	Staphylococcus aureus	547:567	Staphylococcus aureus (P < 0·05)	547:578	METHODS AND RESULTS WfE supplementation at 0·5 and 1 mg ml-1 stimulated probiotic growth (P < 0·05), enhanced adhesion to CaCO2 cells (P < 0·05) while inhibiting foodborne pathogens Escherichia coli and Staphylococcus aureus (P < 0·05).
33973306	3	68	theme	lipid	878:882	arg1	composition					884:894	total cellular lipid composition	863:894	total cellular lipid composition	863:894	1 H-NMR based metabolomic studies indicated higher glucose : lactate and glucose : acetate in the extracellular matrix with significant variation (P < 0·05) in intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition.
33973306	0	69	theme	intracellular	141:153	arg1	pool					184:187	adapted intracellular and extracellular metabolite pool	133:187	adapted intracellular and extracellular metabolite pool	133:187	Woodfordia fruticosa extract supplementation stimulates the growth of Lacticaseibacillus casei and Lacticaseibacillus rhamnosus with adapted intracellular and extracellular metabolite pool.
33973306	7	70	theme	representative	1437:1450	arg1	probiotics					1452:1461	gut representative probiotics	1433:1461	gut representative probiotics	1433:1461	SIGNIFICANCE AND IMPACT OF THE STUDY This is the first report providing insights into the prebiotic-like activity of WfE on gut representative probiotics.
33973306	8	71	theme	extended	1468:1475	arg1	studies					1489:1495	The extended metabolomic studies	1464:1495	The extended metabolomic studies	1464:1495	The extended metabolomic studies shed light on the positive interaction between phytonutrients and beneficial bacteria that possibly help them to adapt to a phytonutrient-rich WfE environment.
33973306	3	72	from	 acetate	663:670	arg1	matrix					693:698	the extracellular matrix	675:698	the extracellular matrix	675:698	1 H-NMR based metabolomic studies indicated higher glucose : lactate and glucose : acetate in the extracellular matrix with significant variation (P < 0·05) in intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition.
33973306	0	73	theme	fruticosa	11:19	arg1	supplementation					29:43	Woodfordia fruticosa extract supplementation	0:43	Woodfordia fruticosa extract supplementation	0:43	Woodfordia fruticosa extract supplementation stimulates the growth of Lacticaseibacillus casei and Lacticaseibacillus rhamnosus with adapted intracellular and extracellular metabolite pool.
33973306	5	74	theme	PCA	1017:1019	arg1	plots					1021:1025	PCA plots	1017:1025	PCA plots	1017:1025	PCA plots indicated clear discrimination between test groups, highlighting variation in metabolite pool in response to WfE supplementation.
33973306	1	75	dep	AIM	190:192	arg1	rhamnosus					333:341	Lacticaseibacillus rhamnosus	314:341	Lacticaseibacillus rhamnosus	314:341	AIM To investigate the effect of Woodfordia fruticosa extract (WfE) on two probiotic bacteria: Lacticaseibacillus casei and Lacticaseibacillus rhamnosus.
33973306	1	75	dep	AIM	190:192	arg1	casei					304:308	Lacticaseibacillus casei	285:308	Lacticaseibacillus casei	285:308	AIM To investigate the effect of Woodfordia fruticosa extract (WfE) on two probiotic bacteria: Lacticaseibacillus casei and Lacticaseibacillus rhamnosus.
33973306	1	75	dep	AIM	190:192	arg1	investigate					197:207	investigate	197:207	To investigate the effect of Woodfordia fruticosa extract (WfE) on two probiotic bacteria	194:282	AIM To investigate the effect of Woodfordia fruticosa extract (WfE) on two probiotic bacteria: Lacticaseibacillus casei and Lacticaseibacillus rhamnosus.
33973306	9	76	theme	novel	1731:1735	arg1	products					1758:1765	novel synbiotic functional products	1731:1765	novel synbiotic functional products targeting gut microbial modulation to improve health	1731:1818	WfE with potential prebiotic attributes can be used in the development of novel synbiotic functional products targeting gut microbial modulation to improve health.
33973306	7	77	theme	prebiotic-like	1399:1412	arg1	activity					1414:1421	the prebiotic-like activity	1395:1421	the prebiotic-like activity of WfE on gut representative probiotics	1395:1461	SIGNIFICANCE AND IMPACT OF THE STUDY This is the first report providing insights into the prebiotic-like activity of WfE on gut representative probiotics.
33973306	6	78	theme	Phytonutrient-rich	1168:1185	arg1	WfE					1187:1189	CONCLUSION Phytonutrient-rich WfE	1157:1189	CONCLUSION Phytonutrient-rich WfE	1157:1189	CONCLUSION Phytonutrient-rich WfE exhibited prebiotic-like attributes, and probiotic bacteria showed altered metabolite pools as an adaptive mechanism.
33973306	5	79	theme	metabolite	1105:1114	arg1	pool					1116:1119	metabolite pool	1105:1119	metabolite pool	1105:1119	PCA plots indicated clear discrimination between test groups, highlighting variation in metabolite pool in response to WfE supplementation.
33973306	7	80	from	activity	1414:1421	arg1	probiotics					1452:1461	gut representative probiotics	1433:1461	gut representative probiotics	1433:1461	SIGNIFICANCE AND IMPACT OF THE STUDY This is the first report providing insights into the prebiotic-like activity of WfE on gut representative probiotics.
33973306	7	81	dep	SIGNIFICANCE	1309:1320	arg1	This					1346:1349	This	1346:1349	This	1346:1349	SIGNIFICANCE AND IMPACT OF THE STUDY This is the first report providing insights into the prebiotic-like activity of WfE on gut representative probiotics.
33973306	5	82	theme	test	1066:1069	arg1	groups					1071:1076	test groups	1066:1076	test groups	1066:1076	PCA plots indicated clear discrimination between test groups, highlighting variation in metabolite pool in response to WfE supplementation.
33973306	3	83	theme	significant	705:715	arg1	variation					717:725	significant variation	705:725	significant variation (P < 0·05) in intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition	705:894	1 H-NMR based metabolomic studies indicated higher glucose : lactate and glucose : acetate in the extracellular matrix with significant variation (P < 0·05) in intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition.
33973306	3	83	theme	significant	705:715	arg1	P < 0·05					728:735	P < 0·05	728:735	P < 0·05	728:735	1 H-NMR based metabolomic studies indicated higher glucose : lactate and glucose : acetate in the extracellular matrix with significant variation (P < 0·05) in intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition.
33973306	9	84	theme	microbial	1781:1789	arg1	modulation					1791:1800	gut microbial modulation	1777:1800	gut microbial modulation to improve health	1777:1818	WfE with potential prebiotic attributes can be used in the development of novel synbiotic functional products targeting gut microbial modulation to improve health.
33973306	7	85	theme	first	1358:1362	arg1	IMPACT					1326:1331	IMPACT	1326:1331	IMPACT	1326:1331	SIGNIFICANCE AND IMPACT OF THE STUDY This is the first report providing insights into the prebiotic-like activity of WfE on gut representative probiotics.
33973306	7	85	theme	first	1358:1362	arg1	report					1364:1369	the first report	1354:1369	the first report providing insights into the prebiotic-like activity of WfE on gut representative probiotics	1354:1461	SIGNIFICANCE AND IMPACT OF THE STUDY This is the first report providing insights into the prebiotic-like activity of WfE on gut representative probiotics.
33973306	7	85	theme	first	1358:1362	arg1	SIGNIFICANCE					1309:1320	SIGNIFICANCE	1309:1320	SIGNIFICANCE	1309:1320	SIGNIFICANCE AND IMPACT OF THE STUDY This is the first report providing insights into the prebiotic-like activity of WfE on gut representative probiotics.
33973306	3	86	from	variation	717:725	arg1	concentrations					755:768	intracellular concentrations	741:768	intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition	741:894	1 H-NMR based metabolomic studies indicated higher glucose : lactate and glucose : acetate in the extracellular matrix with significant variation (P < 0·05) in intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition.
33973306	3	87	theme	intracellular	741:753	arg1	concentrations					755:768	intracellular concentrations	741:768	intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition	741:894	1 H-NMR based metabolomic studies indicated higher glucose : lactate and glucose : acetate in the extracellular matrix with significant variation (P < 0·05) in intracellular concentrations of lactate, acetate, glutamate, dimethylamine, phenylalanine, branched-chain amino acids and total cellular lipid composition.
33973306	8	88	theme	beneficial	1563:1572	arg1	bacteria					1574:1581	beneficial bacteria	1563:1581	beneficial bacteria	1563:1581	The extended metabolomic studies shed light on the positive interaction between phytonutrients and beneficial bacteria that possibly help them to adapt to a phytonutrient-rich WfE environment.
33973306	2	89	theme	probiotic	416:424	arg1	P < 0·05					434:441	P < 0·05	434:441	P < 0·05	434:441	METHODS AND RESULTS WfE supplementation at 0·5 and 1 mg ml-1 stimulated probiotic growth (P < 0·05), enhanced adhesion to CaCO2 cells (P < 0·05) while inhibiting foodborne pathogens Escherichia coli and Staphylococcus aureus (P < 0·05).
33973306	2	89	theme	probiotic	416:424	arg1	growth					426:431	probiotic growth	416:431	probiotic growth (P < 0·05)	416:442	METHODS AND RESULTS WfE supplementation at 0·5 and 1 mg ml-1 stimulated probiotic growth (P < 0·05), enhanced adhesion to CaCO2 cells (P < 0·05) while inhibiting foodborne pathogens Escherichia coli and Staphylococcus aureus (P < 0·05).
33973306	1	90	theme	probiotic	265:273	arg1	bacteria					275:282	two probiotic bacteria	261:282	two probiotic bacteria	261:282	AIM To investigate the effect of Woodfordia fruticosa extract (WfE) on two probiotic bacteria: Lacticaseibacillus casei and Lacticaseibacillus rhamnosus.
33322449	0	0	theme	Enantiomeric	69:80	arg1	Impurity					82:89	Minor Enantiomeric Impurity	63:89	Minor Enantiomeric Impurity in Ketoprofen and Enantiomeric Purity Evaluation of Commercially Available Dexketoprofen Formulations	63:191	The Effect of Enantiomer Elution Order on the Determination of Minor Enantiomeric Impurity in Ketoprofen and Enantiomeric Purity Evaluation of Commercially Available Dexketoprofen Formulations.
33322449	3	1	theme	enantiomeric	765:776	arg1	impurity					778:785	enantiomeric impurity	765:785	enantiomeric impurity in six marketed dexketoprofen formulations from various vendors	765:849	The validated methods with two alternative elution orders for enantiomers were applied for the evaluation of enantiomeric impurity in six marketed dexketoprofen formulations from various vendors.
33322449	4	2	theme	enantiomeric	908:919	arg1	impurity					921:928	enantiomeric impurity	908:928	enantiomeric impurity	908:928	In most of these formulations except one the content of enantiomeric impurity exceeded 0.1% (w/w).
33322449	0	3	theme	Minor	63:67	arg1	Impurity					82:89	Minor Enantiomeric Impurity	63:89	Minor Enantiomeric Impurity in Ketoprofen and Enantiomeric Purity Evaluation of Commercially Available Dexketoprofen Formulations	63:191	The Effect of Enantiomer Elution Order on the Determination of Minor Enantiomeric Impurity in Ketoprofen and Enantiomeric Purity Evaluation of Commercially Available Dexketoprofen Formulations.
33322449	0	4	from	Effect	4:9	arg1	Determination					46:58	the Determination	42:58	the Determination of Minor Enantiomeric Impurity in Ketoprofen and Enantiomeric Purity Evaluation of Commercially Available Dexketoprofen Formulations	42:191	The Effect of Enantiomer Elution Order on the Determination of Minor Enantiomeric Impurity in Ketoprofen and Enantiomeric Purity Evaluation of Commercially Available Dexketoprofen Formulations.
33322449	2	5	theme	impurity	617:624	arg1	determination					581:593	the determination	577:593	the determination of minor enantiomeric impurity in dexketoprofen	577:641	In the present study, the influence of this uncommon effect on method validation parameters for the determination of minor enantiomeric impurity in dexketoprofen was studied.
33322449	3	6	theme	validated	660:668	arg1	methods					670:676	The validated methods	656:676	The validated methods with two alternative elution orders for enantiomers	656:728	The validated methods with two alternative elution orders for enantiomers were applied for the evaluation of enantiomeric impurity in six marketed dexketoprofen formulations from various vendors.
33322449	1	7	theme	ketoprofen	264:273	arg1	enantiomers					275:285	ketoprofen enantiomers	264:285	ketoprofen enantiomers	264:285	In a recent study, opposite enantiomer elution order was observed for ketoprofen enantiomers on two amylose-phenylcarbamate-based chiral columns with the same chemical composition of the chiral selector but in one case with coated while in the other with an immobilized chiral selector.
33322449	2	8	theme	effect	534:539	arg1	influence					507:515	the influence	503:515	the influence of this uncommon effect on method validation parameters for the determination of minor enantiomeric impurity in dexketoprofen	503:641	In the present study, the influence of this uncommon effect on method validation parameters for the determination of minor enantiomeric impurity in dexketoprofen was studied.
33322449	1	9	theme	chiral	381:386	arg1	selector					388:395	the chiral selector	377:395	the chiral selector	377:395	In a recent study, opposite enantiomer elution order was observed for ketoprofen enantiomers on two amylose-phenylcarbamate-based chiral columns with the same chemical composition of the chiral selector but in one case with coated while in the other with an immobilized chiral selector.
33322449	2	10	theme	enantiomeric	604:615	arg1	impurity					617:624	minor enantiomeric impurity	598:624	minor enantiomeric impurity in dexketoprofen	598:641	In the present study, the influence of this uncommon effect on method validation parameters for the determination of minor enantiomeric impurity in dexketoprofen was studied.
33322449	1	11	theme	immobilized	452:462	arg1	selector					471:478	an immobilized chiral selector	449:478	an immobilized chiral selector	449:478	In a recent study, opposite enantiomer elution order was observed for ketoprofen enantiomers on two amylose-phenylcarbamate-based chiral columns with the same chemical composition of the chiral selector but in one case with coated while in the other with an immobilized chiral selector.
33322449	0	12	theme	Impurity	82:89	arg1	Determination					46:58	the Determination	42:58	the Determination of Minor Enantiomeric Impurity in Ketoprofen and Enantiomeric Purity Evaluation of Commercially Available Dexketoprofen Formulations	42:191	The Effect of Enantiomer Elution Order on the Determination of Minor Enantiomeric Impurity in Ketoprofen and Enantiomeric Purity Evaluation of Commercially Available Dexketoprofen Formulations.
33322449	1	13	theme	selector	388:395	arg1	composition					362:372	the same chemical composition	344:372	the same chemical composition of the chiral selector	344:395	In a recent study, opposite enantiomer elution order was observed for ketoprofen enantiomers on two amylose-phenylcarbamate-based chiral columns with the same chemical composition of the chiral selector but in one case with coated while in the other with an immobilized chiral selector.
33322449	2	14	theme	minor	598:602	arg1	impurity					617:624	minor enantiomeric impurity	598:624	minor enantiomeric impurity in dexketoprofen	598:641	In the present study, the influence of this uncommon effect on method validation parameters for the determination of minor enantiomeric impurity in dexketoprofen was studied.
33322449	1	15	theme	chiral	464:469	arg1	selector					471:478	an immobilized chiral selector	449:478	an immobilized chiral selector	449:478	In a recent study, opposite enantiomer elution order was observed for ketoprofen enantiomers on two amylose-phenylcarbamate-based chiral columns with the same chemical composition of the chiral selector but in one case with coated while in the other with an immobilized chiral selector.
33322449	3	16	with	methods	670:676	arg1	orders					707:712	two alternative elution orders	683:712	two alternative elution orders	683:712	The validated methods with two alternative elution orders for enantiomers were applied for the evaluation of enantiomeric impurity in six marketed dexketoprofen formulations from various vendors.
33322449	1	17	with	other	438:442	arg1	selector					471:478	an immobilized chiral selector	449:478	an immobilized chiral selector	449:478	In a recent study, opposite enantiomer elution order was observed for ketoprofen enantiomers on two amylose-phenylcarbamate-based chiral columns with the same chemical composition of the chiral selector but in one case with coated while in the other with an immobilized chiral selector.
33322449	2	18	from	determination	581:593	arg1	dexketoprofen					629:641	dexketoprofen	629:641	dexketoprofen	629:641	In the present study, the influence of this uncommon effect on method validation parameters for the determination of minor enantiomeric impurity in dexketoprofen was studied.
33322449	2	19	theme	validation	551:560	arg1	parameters					562:571	method validation parameters	544:571	method validation parameters for the determination of minor enantiomeric impurity in dexketoprofen	544:641	In the present study, the influence of this uncommon effect on method validation parameters for the determination of minor enantiomeric impurity in dexketoprofen was studied.
33322449	2	20	theme	present	488:494	arg1	study					496:500	the present study	484:500	the present study	484:500	In the present study, the influence of this uncommon effect on method validation parameters for the determination of minor enantiomeric impurity in dexketoprofen was studied.
33322449	3	21	theme	dexketoprofen	803:815	arg1	formulations					817:828	six marketed dexketoprofen formulations	790:828	six marketed dexketoprofen formulations from various vendors	790:849	The validated methods with two alternative elution orders for enantiomers were applied for the evaluation of enantiomeric impurity in six marketed dexketoprofen formulations from various vendors.
33322449	3	22	theme	marketed	794:801	arg1	formulations					817:828	six marketed dexketoprofen formulations	790:828	six marketed dexketoprofen formulations from various vendors	790:849	The validated methods with two alternative elution orders for enantiomers were applied for the evaluation of enantiomeric impurity in six marketed dexketoprofen formulations from various vendors.
33322449	3	23	theme	impurity	778:785	arg1	evaluation					751:760	the evaluation	747:760	the evaluation of enantiomeric impurity in six marketed dexketoprofen formulations from various vendors	747:849	The validated methods with two alternative elution orders for enantiomers were applied for the evaluation of enantiomeric impurity in six marketed dexketoprofen formulations from various vendors.
33322449	1	24	with	columns	331:337	arg1	composition					362:372	the same chemical composition	344:372	the same chemical composition of the chiral selector	344:395	In a recent study, opposite enantiomer elution order was observed for ketoprofen enantiomers on two amylose-phenylcarbamate-based chiral columns with the same chemical composition of the chiral selector but in one case with coated while in the other with an immobilized chiral selector.
33322449	3	25	theme	alternative	687:697	arg1	orders					707:712	two alternative elution orders	683:712	two alternative elution orders	683:712	The validated methods with two alternative elution orders for enantiomers were applied for the evaluation of enantiomeric impurity in six marketed dexketoprofen formulations from various vendors.
33322449	1	26	theme	recent	199:204	arg1	study					206:210	a recent study	197:210	a recent study	197:210	In a recent study, opposite enantiomer elution order was observed for ketoprofen enantiomers on two amylose-phenylcarbamate-based chiral columns with the same chemical composition of the chiral selector but in one case with coated while in the other with an immobilized chiral selector.
33322449	0	27	theme	Enantiomer	14:23	arg1	Order					33:37	Enantiomer Elution Order	14:37	Enantiomer Elution Order	14:37	The Effect of Enantiomer Elution Order on the Determination of Minor Enantiomeric Impurity in Ketoprofen and Enantiomeric Purity Evaluation of Commercially Available Dexketoprofen Formulations.
33322449	1	28	theme	amylose-phenylcarbamate-based	294:322	arg1	columns					331:337	two amylose-phenylcarbamate-based chiral columns	290:337	two amylose-phenylcarbamate-based chiral columns with the same chemical composition of the chiral selector	290:395	In a recent study, opposite enantiomer elution order was observed for ketoprofen enantiomers on two amylose-phenylcarbamate-based chiral columns with the same chemical composition of the chiral selector but in one case with coated while in the other with an immobilized chiral selector.
33322449	0	29	theme	Purity	122:127	arg1	Evaluation					129:138	Enantiomeric Purity Evaluation	109:138	Enantiomeric Purity Evaluation	109:138	The Effect of Enantiomer Elution Order on the Determination of Minor Enantiomeric Impurity in Ketoprofen and Enantiomeric Purity Evaluation of Commercially Available Dexketoprofen Formulations.
33322449	1	30	theme	chiral	324:329	arg1	columns					331:337	two amylose-phenylcarbamate-based chiral columns	290:337	two amylose-phenylcarbamate-based chiral columns with the same chemical composition of the chiral selector	290:395	In a recent study, opposite enantiomer elution order was observed for ketoprofen enantiomers on two amylose-phenylcarbamate-based chiral columns with the same chemical composition of the chiral selector but in one case with coated while in the other with an immobilized chiral selector.
33322449	0	31	theme	Enantiomeric	109:120	arg1	Evaluation					129:138	Enantiomeric Purity Evaluation	109:138	Enantiomeric Purity Evaluation	109:138	The Effect of Enantiomer Elution Order on the Determination of Minor Enantiomeric Impurity in Ketoprofen and Enantiomeric Purity Evaluation of Commercially Available Dexketoprofen Formulations.
33322449	1	32	with	case	408:411	arg1	coated					418:423	coated	418:423	coated	418:423	In a recent study, opposite enantiomer elution order was observed for ketoprofen enantiomers on two amylose-phenylcarbamate-based chiral columns with the same chemical composition of the chiral selector but in one case with coated while in the other with an immobilized chiral selector.
33322449	0	33	theme	Order	33:37	arg1	Effect					4:9	The Effect	0:9	The Effect of Enantiomer Elution Order on the Determination of Minor Enantiomeric Impurity in Ketoprofen and Enantiomeric Purity Evaluation of Commercially Available Dexketoprofen Formulations.	0:192	The Effect of Enantiomer Elution Order on the Determination of Minor Enantiomeric Impurity in Ketoprofen and Enantiomeric Purity Evaluation of Commercially Available Dexketoprofen Formulations.
33322449	2	34	from	impurity	617:624	arg1	dexketoprofen					629:641	dexketoprofen	629:641	dexketoprofen	629:641	In the present study, the influence of this uncommon effect on method validation parameters for the determination of minor enantiomeric impurity in dexketoprofen was studied.
33322449	2	35	theme	method	544:549	arg1	parameters					562:571	method validation parameters	544:571	method validation parameters for the determination of minor enantiomeric impurity in dexketoprofen	544:641	In the present study, the influence of this uncommon effect on method validation parameters for the determination of minor enantiomeric impurity in dexketoprofen was studied.
33322449	1	36	theme	opposite	213:220	arg1	order					241:245	opposite enantiomer elution order	213:245	opposite enantiomer elution order	213:245	In a recent study, opposite enantiomer elution order was observed for ketoprofen enantiomers on two amylose-phenylcarbamate-based chiral columns with the same chemical composition of the chiral selector but in one case with coated while in the other with an immobilized chiral selector.
33322449	0	37	theme	Elution	25:31	arg1	Order					33:37	Enantiomer Elution Order	14:37	Enantiomer Elution Order	14:37	The Effect of Enantiomer Elution Order on the Determination of Minor Enantiomeric Impurity in Ketoprofen and Enantiomeric Purity Evaluation of Commercially Available Dexketoprofen Formulations.
33322449	2	38	from	influence	507:515	arg1	parameters					562:571	method validation parameters	544:571	method validation parameters for the determination of minor enantiomeric impurity in dexketoprofen	544:641	In the present study, the influence of this uncommon effect on method validation parameters for the determination of minor enantiomeric impurity in dexketoprofen was studied.
33322449	3	39	from	evaluation	751:760	arg1	formulations					817:828	six marketed dexketoprofen formulations	790:828	six marketed dexketoprofen formulations from various vendors	790:849	The validated methods with two alternative elution orders for enantiomers were applied for the evaluation of enantiomeric impurity in six marketed dexketoprofen formulations from various vendors.
33322449	1	40	theme	enantiomer	222:231	arg1	order					241:245	opposite enantiomer elution order	213:245	opposite enantiomer elution order	213:245	In a recent study, opposite enantiomer elution order was observed for ketoprofen enantiomers on two amylose-phenylcarbamate-based chiral columns with the same chemical composition of the chiral selector but in one case with coated while in the other with an immobilized chiral selector.
33322449	2	41	from	dexketoprofen	629:641	arg1	determination					581:593	the determination	577:593	the determination of minor enantiomeric impurity in dexketoprofen	577:641	In the present study, the influence of this uncommon effect on method validation parameters for the determination of minor enantiomeric impurity in dexketoprofen was studied.
33322449	0	42	theme	Available	156:164	arg1	Formulations					180:191	Commercially Available Dexketoprofen Formulations	143:191	Commercially Available Dexketoprofen Formulations	143:191	The Effect of Enantiomer Elution Order on the Determination of Minor Enantiomeric Impurity in Ketoprofen and Enantiomeric Purity Evaluation of Commercially Available Dexketoprofen Formulations.
33322449	0	43	from	Determination	46:58	arg1	Evaluation					129:138	Enantiomeric Purity Evaluation	109:138	Enantiomeric Purity Evaluation	109:138	The Effect of Enantiomer Elution Order on the Determination of Minor Enantiomeric Impurity in Ketoprofen and Enantiomeric Purity Evaluation of Commercially Available Dexketoprofen Formulations.
33322449	0	43	from	Determination	46:58	arg1	Ketoprofen					94:103	Ketoprofen	94:103	Ketoprofen	94:103	The Effect of Enantiomer Elution Order on the Determination of Minor Enantiomeric Impurity in Ketoprofen and Enantiomeric Purity Evaluation of Commercially Available Dexketoprofen Formulations.
33322449	1	44	located	observed	251:258	arg1	columns					331:337	two amylose-phenylcarbamate-based chiral columns	290:337	two amylose-phenylcarbamate-based chiral columns with the same chemical composition of the chiral selector	290:395	In a recent study, opposite enantiomer elution order was observed for ketoprofen enantiomers on two amylose-phenylcarbamate-based chiral columns with the same chemical composition of the chiral selector but in one case with coated while in the other with an immobilized chiral selector.
33322449	1	44	located	observed	251:258	arg1	study					206:210	a recent study	197:210	a recent study	197:210	In a recent study, opposite enantiomer elution order was observed for ketoprofen enantiomers on two amylose-phenylcarbamate-based chiral columns with the same chemical composition of the chiral selector but in one case with coated while in the other with an immobilized chiral selector.
33322449	1	44	located	observed	251:258	arg2	order					241:245	opposite enantiomer elution order	213:245	opposite enantiomer elution order	213:245	In a recent study, opposite enantiomer elution order was observed for ketoprofen enantiomers on two amylose-phenylcarbamate-based chiral columns with the same chemical composition of the chiral selector but in one case with coated while in the other with an immobilized chiral selector.
33322449	1	45	theme	same	348:351	arg1	composition					362:372	the same chemical composition	344:372	the same chemical composition of the chiral selector	344:395	In a recent study, opposite enantiomer elution order was observed for ketoprofen enantiomers on two amylose-phenylcarbamate-based chiral columns with the same chemical composition of the chiral selector but in one case with coated while in the other with an immobilized chiral selector.
33322449	3	46	from	vendors	843:849	arg1	formulations					817:828	six marketed dexketoprofen formulations	790:828	six marketed dexketoprofen formulations from various vendors	790:849	The validated methods with two alternative elution orders for enantiomers were applied for the evaluation of enantiomeric impurity in six marketed dexketoprofen formulations from various vendors.
33322449	3	46	from	vendors	843:849	arg1	impurity					778:785	enantiomeric impurity	765:785	enantiomeric impurity in six marketed dexketoprofen formulations from various vendors	765:849	The validated methods with two alternative elution orders for enantiomers were applied for the evaluation of enantiomeric impurity in six marketed dexketoprofen formulations from various vendors.
33322449	3	47	theme	elution	699:705	arg1	orders					707:712	two alternative elution orders	683:712	two alternative elution orders	683:712	The validated methods with two alternative elution orders for enantiomers were applied for the evaluation of enantiomeric impurity in six marketed dexketoprofen formulations from various vendors.
33322449	3	48	from	impurity	778:785	arg1	formulations					817:828	six marketed dexketoprofen formulations	790:828	six marketed dexketoprofen formulations from various vendors	790:849	The validated methods with two alternative elution orders for enantiomers were applied for the evaluation of enantiomeric impurity in six marketed dexketoprofen formulations from various vendors.
33322449	3	48	from	impurity	778:785	arg1	vendors					843:849	various vendors	835:849	various vendors	835:849	The validated methods with two alternative elution orders for enantiomers were applied for the evaluation of enantiomeric impurity in six marketed dexketoprofen formulations from various vendors.
33322449	3	49	from	formulations	817:828	arg1	evaluation					751:760	the evaluation	747:760	the evaluation of enantiomeric impurity in six marketed dexketoprofen formulations from various vendors	747:849	The validated methods with two alternative elution orders for enantiomers were applied for the evaluation of enantiomeric impurity in six marketed dexketoprofen formulations from various vendors.
33322449	1	50	theme	chemical	353:360	arg1	composition					362:372	the same chemical composition	344:372	the same chemical composition of the chiral selector	344:395	In a recent study, opposite enantiomer elution order was observed for ketoprofen enantiomers on two amylose-phenylcarbamate-based chiral columns with the same chemical composition of the chiral selector but in one case with coated while in the other with an immobilized chiral selector.
33322449	0	51	theme	Formulations	180:191	arg1	Evaluation					129:138	Enantiomeric Purity Evaluation	109:138	Enantiomeric Purity Evaluation	109:138	The Effect of Enantiomer Elution Order on the Determination of Minor Enantiomeric Impurity in Ketoprofen and Enantiomeric Purity Evaluation of Commercially Available Dexketoprofen Formulations.
33322449	0	51	theme	Formulations	180:191	arg1	Ketoprofen					94:103	Ketoprofen	94:103	Ketoprofen	94:103	The Effect of Enantiomer Elution Order on the Determination of Minor Enantiomeric Impurity in Ketoprofen and Enantiomeric Purity Evaluation of Commercially Available Dexketoprofen Formulations.
33322449	0	52	from	Ketoprofen	94:103	arg1	Determination					46:58	the Determination	42:58	the Determination of Minor Enantiomeric Impurity in Ketoprofen and Enantiomeric Purity Evaluation of Commercially Available Dexketoprofen Formulations	42:191	The Effect of Enantiomer Elution Order on the Determination of Minor Enantiomeric Impurity in Ketoprofen and Enantiomeric Purity Evaluation of Commercially Available Dexketoprofen Formulations.
33322449	1	53	theme	elution	233:239	arg1	order					241:245	opposite enantiomer elution order	213:245	opposite enantiomer elution order	213:245	In a recent study, opposite enantiomer elution order was observed for ketoprofen enantiomers on two amylose-phenylcarbamate-based chiral columns with the same chemical composition of the chiral selector but in one case with coated while in the other with an immobilized chiral selector.
33322449	0	54	theme	Dexketoprofen	166:178	arg1	Formulations					180:191	Commercially Available Dexketoprofen Formulations	143:191	Commercially Available Dexketoprofen Formulations	143:191	The Effect of Enantiomer Elution Order on the Determination of Minor Enantiomeric Impurity in Ketoprofen and Enantiomeric Purity Evaluation of Commercially Available Dexketoprofen Formulations.
33322449	4	55	theme	impurity	921:928	arg1	content					897:903	the content	893:903	the content of enantiomeric impurity	893:928	In most of these formulations except one the content of enantiomeric impurity exceeded 0.1% (w/w).
33322449	0	56	from	Evaluation	129:138	arg1	Determination					46:58	the Determination	42:58	the Determination of Minor Enantiomeric Impurity in Ketoprofen and Enantiomeric Purity Evaluation of Commercially Available Dexketoprofen Formulations	42:191	The Effect of Enantiomer Elution Order on the Determination of Minor Enantiomeric Impurity in Ketoprofen and Enantiomeric Purity Evaluation of Commercially Available Dexketoprofen Formulations.
33322449	0	57	from	Impurity	82:89	arg1	Evaluation					129:138	Enantiomeric Purity Evaluation	109:138	Enantiomeric Purity Evaluation	109:138	The Effect of Enantiomer Elution Order on the Determination of Minor Enantiomeric Impurity in Ketoprofen and Enantiomeric Purity Evaluation of Commercially Available Dexketoprofen Formulations.
33322449	0	57	from	Impurity	82:89	arg1	Ketoprofen					94:103	Ketoprofen	94:103	Ketoprofen	94:103	The Effect of Enantiomer Elution Order on the Determination of Minor Enantiomeric Impurity in Ketoprofen and Enantiomeric Purity Evaluation of Commercially Available Dexketoprofen Formulations.
33322449	3	58	theme	various	835:841	arg1	vendors					843:849	various vendors	835:849	various vendors	835:849	The validated methods with two alternative elution orders for enantiomers were applied for the evaluation of enantiomeric impurity in six marketed dexketoprofen formulations from various vendors.
33322449	2	59	theme	uncommon	525:532	arg1	effect					534:539	this uncommon effect	520:539	this uncommon effect	520:539	In the present study, the influence of this uncommon effect on method validation parameters for the determination of minor enantiomeric impurity in dexketoprofen was studied.
34690938	0	0	theme	Gut	85:87	arg1	Genomes					103:109	Human Gut Bacteroidetes Genomes	79:109	Human Gut Bacteroidetes Genomes	79:109	FiberGrowth Pipeline: A Framework Toward Predicting Fiber-Specific Growth From Human Gut Bacteroidetes Genomes.
34690938	7	1	theme	gene	1035:1038	arg1	data					1051:1054	published growth and gene expression data	1014:1054	data	1051:1054	To do so, we compared the existing GH annotation tools and built PUL models by using published growth and gene expression data.
34690938	8	2	theme	heparin	1269:1275	arg1	0.17					1312:1315	0.17	1312:1315	0.17	1312:1315	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	8	2	theme	heparin	1269:1275	arg1	0.38					1303:1306	0.38	1303:1306	0.38	1303:1306	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	8	2	theme	heparin	1269:1275	arg1	laminarin					1292:1300	heparin (0.8 and 0.22) laminarin	1269:1300	heparin (0.8 and 0.22) laminarin (0.38 and 0.17)	1269:1316	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	0	3	theme	Human	79:83	arg1	Genomes					103:109	Human Gut Bacteroidetes Genomes	79:109	Human Gut Bacteroidetes Genomes	79:109	FiberGrowth Pipeline: A Framework Toward Predicting Fiber-Specific Growth From Human Gut Bacteroidetes Genomes.
34690938	4	4	theme	glycan	483:488	arg1	breakdown					490:498	glycan breakdown	483:498	glycan breakdown (adhesion, hydrolysis, and fermentation)	483:539	However, the GH involved in glycan breakdown (adhesion, hydrolysis, and fermentation) are organized in polysaccharide utilization loci (PUL) with complex modularity.
34690938	7	5	theme	expression	1040:1049	arg1	data					1051:1054	published growth and gene expression data	1014:1054	data	1051:1054	To do so, we compared the existing GH annotation tools and built PUL models by using published growth and gene expression data.
34690938	8	6	dep	mucus	1341:1345	arg1	0.38					1357:1360	0.38	1357:1360	0.38	1357:1360	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	8	6	dep	mucus	1341:1345	arg1	0.13					1348:1351	0.13	1348:1351	0.13	1348:1351	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	2	7	attach	linked	231:236	arg2	diet					217:220	A low-fiber diet	205:220	A low-fiber diet	205:220	A low-fiber diet has been linked to lower bacterial diversity, obesity, type 2 diabetes, and promotion of mucosal pathogens.
34690938	2	7	attach	linked	231:236	arg1	promotion					298:306	promotion	298:306	promotion of mucosal pathogens	298:327	A low-fiber diet has been linked to lower bacterial diversity, obesity, type 2 diabetes, and promotion of mucosal pathogens.
34690938	2	7	attach	linked	231:236	arg1	diversity					257:265	lower bacterial diversity	241:265	lower bacterial diversity	241:265	A low-fiber diet has been linked to lower bacterial diversity, obesity, type 2 diabetes, and promotion of mucosal pathogens.
34690938	2	7	attach	linked	231:236	arg1	obesity					268:274	obesity	268:274	obesity	268:274	A low-fiber diet has been linked to lower bacterial diversity, obesity, type 2 diabetes, and promotion of mucosal pathogens.
34690938	2	7	attach	linked	231:236	arg1	diabetes					284:291	type 2 diabetes	277:291	type 2 diabetes	277:291	A low-fiber diet has been linked to lower bacterial diversity, obesity, type 2 diabetes, and promotion of mucosal pathogens.
34690938	9	8	theme	human	1499:1503	arg1	nutrition					1505:1513	human nutrition	1499:1513	human nutrition	1499:1513	Being able to better predict fiber breakdown by bacterial strains would help to understand their impact on human nutrition and health.
34690938	4	9	dep	breakdown	490:498	arg1	adhesion					501:508	adhesion	501:508	adhesion	501:508	However, the GH involved in glycan breakdown (adhesion, hydrolysis, and fermentation) are organized in polysaccharide utilization loci (PUL) with complex modularity.
34690938	4	9	dep	breakdown	490:498	arg1	hydrolysis					511:520	hydrolysis	511:520	hydrolysis	511:520	However, the GH involved in glycan breakdown (adhesion, hydrolysis, and fermentation) are organized in polysaccharide utilization loci (PUL) with complex modularity.
34690938	4	9	dep	breakdown	490:498	arg1	fermentation					527:538	fermentation	527:538	fermentation	527:538	However, the GH involved in glycan breakdown (adhesion, hydrolysis, and fermentation) are organized in polysaccharide utilization loci (PUL) with complex modularity.
34690938	2	10	theme	type	277:280	arg1	diabetes					284:291	type 2 diabetes	277:291	type 2 diabetes	277:291	A low-fiber diet has been linked to lower bacterial diversity, obesity, type 2 diabetes, and promotion of mucosal pathogens.
34690938	10	11	theme	structural	1597:1606	arg1	analysis					1608:1615	structural analysis	1597:1615	structural analysis	1597:1615	Assuming further gene expression experiment along with discoveries on structural analysis, we hope computational tools like FiberGrowth will help researchers prioritize and design in vitro experiments.
34690938	0	12	theme	Bacteroidetes	89:101	arg1	Genomes					103:109	Human Gut Bacteroidetes Genomes	79:109	Human Gut Bacteroidetes Genomes	79:109	FiberGrowth Pipeline: A Framework Toward Predicting Fiber-Specific Growth From Human Gut Bacteroidetes Genomes.
34690938	10	13	dep	in	1707:1708	arg1	vitro					1710:1714	vitro	1710:1714	vitro	1710:1714	Assuming further gene expression experiment along with discoveries on structural analysis, we hope computational tools like FiberGrowth will help researchers prioritize and design in vitro experiments.
34690938	9	14	from	impact	1489:1494	arg1	nutrition					1505:1513	human nutrition	1499:1513	human nutrition	1499:1513	Being able to better predict fiber breakdown by bacterial strains would help to understand their impact on human nutrition and health.
34690938	9	14	from	impact	1489:1494	arg1	health					1519:1524	health	1519:1524	health	1519:1524	Being able to better predict fiber breakdown by bacterial strains would help to understand their impact on human nutrition and health.
34690938	5	15	from	capacity	653:660	arg1	Bacteroidetes					683:695	Bacteroidetes	683:695	Bacteroidetes	683:695	Our goal was to explore how the capacity of strains, from the Bacteroidetes phylum, to grow on fiber could be predicted from their genome sequences.
34690938	8	16	theme	available	1182:1190	arg1	data					1192:1195	available data	1182:1195	available data	1182:1195	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	3	17	theme	bacterial	395:403	arg1	catabolism					405:414	the bacterial catabolism	391:414	the bacterial catabolism of fiber into short-chain fatty acids	391:452	Glycoside hydrolases (GHs) are important enzymes involved in the bacterial catabolism of fiber into short-chain fatty acids.
34690938	8	18	theme	positive	1141:1148	arg1	rate					1150:1153	false positive rate	1135:1153	false positive rate (FPR)	1135:1159	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	8	18	theme	positive	1141:1148	arg1	FPR					1156:1158	FPR	1156:1158	FPR	1156:1158	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	3	19	theme	Glycoside	330:338	arg1	GHs					352:354	GHs	352:354	GHs	352:354	Glycoside hydrolases (GHs) are important enzymes involved in the bacterial catabolism of fiber into short-chain fatty acids.
34690938	3	19	theme	Glycoside	330:338	arg1	hydrolases					340:349	Glycoside hydrolases	330:349	Glycoside hydrolases (GHs)	330:355	Glycoside hydrolases (GHs) are important enzymes involved in the bacterial catabolism of fiber into short-chain fatty acids.
34690938	3	19	theme	Glycoside	330:338	arg1	enzymes					371:377	important enzymes	361:377	important enzymes involved in the bacterial catabolism of fiber into short-chain fatty acids	361:452	Glycoside hydrolases (GHs) are important enzymes involved in the bacterial catabolism of fiber into short-chain fatty acids.
34690938	6	20	from	strains	920:926	arg1	genomes					888:894	28 genomes	885:894	28 genomes from Bacteroidetes-type strains	885:926	We designed an in silico pipeline called FiberGrowth and independently validated it for seven different fibers, on 28 genomes from Bacteroidetes-type strains.
34690938	3	21	theme	important	361:369	arg1	hydrolases					340:349	Glycoside hydrolases	330:349	Glycoside hydrolases (GHs)	330:355	Glycoside hydrolases (GHs) are important enzymes involved in the bacterial catabolism of fiber into short-chain fatty acids.
34690938	3	21	theme	important	361:369	arg1	enzymes					371:377	important enzymes	361:377	important enzymes involved in the bacterial catabolism of fiber into short-chain fatty acids	361:452	Glycoside hydrolases (GHs) are important enzymes involved in the bacterial catabolism of fiber into short-chain fatty acids.
34690938	10	22	theme	computational	1626:1638	arg1	tools					1640:1644	computational tools	1626:1644	computational tools like FiberGrowth	1626:1661	Assuming further gene expression experiment along with discoveries on structural analysis, we hope computational tools like FiberGrowth will help researchers prioritize and design in vitro experiments.
34690938	3	23	theme	fiber	419:423	arg1	catabolism					405:414	the bacterial catabolism	391:414	the bacterial catabolism of fiber into short-chain fatty acids	391:452	Glycoside hydrolases (GHs) are important enzymes involved in the bacterial catabolism of fiber into short-chain fatty acids.
34690938	8	24	theme	true	1106:1109	arg1	TPR					1126:1128	TPR	1126:1128	TPR	1126:1128	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	8	24	theme	true	1106:1109	arg1	rate					1120:1123	true positive rate	1106:1123	true positive rate (TPR)	1106:1129	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	7	25	theme	annotation	967:976	arg1	tools					978:982	the existing GH annotation tools	951:982	the existing GH annotation tools	951:982	To do so, we compared the existing GH annotation tools and built PUL models by using published growth and gene expression data.
34690938	1	26	theme	short-chain	180:190	arg1	acids					198:202	short-chain fatty acids	180:202	short-chain fatty acids	180:202	Dietary fibers impact gut colonic health, through the production of short-chain fatty acids.
34690938	0	27	theme	FiberGrowth	0:10	arg1	Pipeline					12:19	FiberGrowth Pipeline	0:19	FiberGrowth Pipeline: A Framework Toward Predicting Fiber-Specific Growth From Human Gut Bacteroidetes Genomes.	0:110	FiberGrowth Pipeline: A Framework Toward Predicting Fiber-Specific Growth From Human Gut Bacteroidetes Genomes.
34690938	6	28	theme	Bacteroidetes-type	901:918	arg1	strains					920:926	Bacteroidetes-type strains	901:926	Bacteroidetes-type strains	901:926	We designed an in silico pipeline called FiberGrowth and independently validated it for seven different fibers, on 28 genomes from Bacteroidetes-type strains.
34690938	10	29	theme	expression	1549:1558	arg1	experiment					1560:1569	further gene expression experiment	1536:1569	further gene expression experiment	1536:1569	Assuming further gene expression experiment along with discoveries on structural analysis, we hope computational tools like FiberGrowth will help researchers prioritize and design in vitro experiments.
34690938	8	30	theme	FPR	1236:1238	arg1	TPR					1222:1224	TPR	1222:1224	TPR: 0.89 and FPR: 0	1222:1241	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	8	30	theme	FPR	1236:1238	arg1	arabinoxylan					1208:1219	arabinoxylan	1208:1219	arabinoxylan (TPR: 0.89 and FPR: 0)	1208:1242	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	1	31	theme	fatty	192:196	arg1	acids					198:202	short-chain fatty acids	180:202	short-chain fatty acids	180:202	Dietary fibers impact gut colonic health, through the production of short-chain fatty acids.
34690938	8	32	theme	rate	1120:1123	arg1	terms					1097:1101	terms	1097:1101	terms of true positive rate (TPR) and false positive rate (FPR)	1097:1159	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	0	33	dep	Pipeline	12:19	arg1	Framework					24:32	A Framework	22:32	FiberGrowth Pipeline: A Framework Toward Predicting Fiber-Specific Growth From Human Gut Bacteroidetes Genomes.	0:110	FiberGrowth Pipeline: A Framework Toward Predicting Fiber-Specific Growth From Human Gut Bacteroidetes Genomes.
34690938	9	34	theme	fiber	1421:1425	arg1	breakdown					1427:1435	fiber breakdown	1421:1435	fiber breakdown	1421:1435	Being able to better predict fiber breakdown by bacterial strains would help to understand their impact on human nutrition and health.
34690938	1	35	theme	acids	198:202	arg1	production					166:175	the production	162:175	the production of short-chain fatty acids	162:202	Dietary fibers impact gut colonic health, through the production of short-chain fatty acids.
34690938	5	36	theme	genome	752:757	arg1	sequences					759:767	their genome sequences	746:767	their genome sequences	746:767	Our goal was to explore how the capacity of strains, from the Bacteroidetes phylum, to grow on fiber could be predicted from their genome sequences.
34690938	10	37	theme	in	1707:1708	arg1	experiments					1716:1726	in vitro experiments	1707:1726	in vitro experiments	1707:1726	Assuming further gene expression experiment along with discoveries on structural analysis, we hope computational tools like FiberGrowth will help researchers prioritize and design in vitro experiments.
34690938	7	38	theme	PUL	994:996	arg1	models					998:1003	PUL models	994:1003	PUL models	994:1003	To do so, we compared the existing GH annotation tools and built PUL models by using published growth and gene expression data.
34690938	1	39	theme	Dietary	112:118	arg1	fibers					120:125	Dietary fibers	112:125	Dietary fibers	112:125	Dietary fibers impact gut colonic health, through the production of short-chain fatty acids.
34690938	8	40	theme	0.89	1227:1230	arg1	TPR					1222:1224	TPR	1222:1224	TPR: 0.89 and FPR: 0	1222:1241	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	8	40	theme	0.89	1227:1230	arg1	arabinoxylan					1208:1219	arabinoxylan	1208:1219	arabinoxylan (TPR: 0.89 and FPR: 0)	1208:1242	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	5	41	from	Bacteroidetes	683:695	arg1	capacity					653:660	the capacity	649:660	the capacity of strains, from the Bacteroidetes phylum, to grow on fiber	649:720	Our goal was to explore how the capacity of strains, from the Bacteroidetes phylum, to grow on fiber could be predicted from their genome sequences.
34690938	5	41	from	Bacteroidetes	683:695	arg1	strains					665:671	strains	665:671	strains	665:671	Our goal was to explore how the capacity of strains, from the Bacteroidetes phylum, to grow on fiber could be predicted from their genome sequences.
34690938	10	42	theme	gene	1544:1547	arg1	experiment					1560:1569	further gene expression experiment	1536:1569	further gene expression experiment	1536:1569	Assuming further gene expression experiment along with discoveries on structural analysis, we hope computational tools like FiberGrowth will help researchers prioritize and design in vitro experiments.
34690938	8	43	theme	rate	1150:1153	arg1	terms					1097:1101	terms	1097:1101	terms of true positive rate (TPR) and false positive rate (FPR)	1097:1159	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	4	44	theme	complex	601:607	arg1	modularity					609:618	complex modularity	601:618	complex modularity	601:618	However, the GH involved in glycan breakdown (adhesion, hydrolysis, and fermentation) are organized in polysaccharide utilization loci (PUL) with complex modularity.
34690938	6	45	dep	in	785:786	arg1	silico					788:793	silico	788:793	silico	788:793	We designed an in silico pipeline called FiberGrowth and independently validated it for seven different fibers, on 28 genomes from Bacteroidetes-type strains.
34690938	5	46	theme	strains	665:671	arg1	capacity					653:660	the capacity	649:660	the capacity of strains, from the Bacteroidetes phylum, to grow on fiber	649:720	Our goal was to explore how the capacity of strains, from the Bacteroidetes phylum, to grow on fiber could be predicted from their genome sequences.
34690938	6	47	theme	in	785:786	arg1	pipeline					795:802	an in silico pipeline	782:802	an in silico pipeline called FiberGrowth and independently validated it for seven different fibers	782:879	We designed an in silico pipeline called FiberGrowth and independently validated it for seven different fibers, on 28 genomes from Bacteroidetes-type strains.
34690938	4	48	with	loci	585:588	arg1	modularity					609:618	complex modularity	601:618	complex modularity	601:618	However, the GH involved in glycan breakdown (adhesion, hydrolysis, and fermentation) are organized in polysaccharide utilization loci (PUL) with complex modularity.
34690938	3	49	theme	short-chain	430:440	arg1	acids					448:452	short-chain fatty acids	430:452	short-chain fatty acids	430:452	Glycoside hydrolases (GHs) are important enzymes involved in the bacterial catabolism of fiber into short-chain fatty acids.
34690938	0	50	theme	Fiber-Specific	52:65	arg1	Growth					67:72	Fiber-Specific Growth	52:72	Fiber-Specific Growth	52:72	FiberGrowth Pipeline: A Framework Toward Predicting Fiber-Specific Growth From Human Gut Bacteroidetes Genomes.
34690938	7	51	theme	GH	964:965	arg1	tools					978:982	the existing GH annotation tools	951:982	the existing GH annotation tools	951:982	To do so, we compared the existing GH annotation tools and built PUL models by using published growth and gene expression data.
34690938	8	52	dep	laminarin	1292:1300	arg1	0.8					1278:1280	0.8	1278:1280	0.8	1278:1280	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	8	52	dep	laminarin	1292:1300	arg1	0.22					1286:1289	0.22	1286:1289	0.22	1286:1289	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	2	53	theme	pathogens	319:327	arg1	diabetes					284:291	type 2 diabetes	277:291	type 2 diabetes	277:291	A low-fiber diet has been linked to lower bacterial diversity, obesity, type 2 diabetes, and promotion of mucosal pathogens.
34690938	2	53	theme	pathogens	319:327	arg1	promotion					298:306	promotion	298:306	promotion of mucosal pathogens	298:327	A low-fiber diet has been linked to lower bacterial diversity, obesity, type 2 diabetes, and promotion of mucosal pathogens.
34690938	2	53	theme	pathogens	319:327	arg1	obesity					268:274	obesity	268:274	obesity	268:274	A low-fiber diet has been linked to lower bacterial diversity, obesity, type 2 diabetes, and promotion of mucosal pathogens.
34690938	2	53	theme	pathogens	319:327	arg1	diversity					257:265	lower bacterial diversity	241:265	lower bacterial diversity	241:265	A low-fiber diet has been linked to lower bacterial diversity, obesity, type 2 diabetes, and promotion of mucosal pathogens.
34690938	9	54	theme	bacterial	1440:1448	arg1	strains					1450:1456	bacterial strains	1440:1456	bacterial strains	1440:1456	Being able to better predict fiber breakdown by bacterial strains would help to understand their impact on human nutrition and health.
34690938	6	55	dep	pipeline	795:802	arg1	called					804:809	called	804:809	called FiberGrowth	804:821	We designed an in silico pipeline called FiberGrowth and independently validated it for seven different fibers, on 28 genomes from Bacteroidetes-type strains.
34690938	6	55	dep	pipeline	795:802	arg1	validated					841:849	validated	841:849	independently validated it for seven different fibers	827:879	We designed an in silico pipeline called FiberGrowth and independently validated it for seven different fibers, on 28 genomes from Bacteroidetes-type strains.
34690938	5	56	dep	Bacteroidetes	683:695	arg1	phylum					697:702	phylum	697:702	phylum	697:702	Our goal was to explore how the capacity of strains, from the Bacteroidetes phylum, to grow on fiber could be predicted from their genome sequences.
34690938	2	57	theme	low-fiber	207:215	arg1	diet					217:220	A low-fiber diet	205:220	A low-fiber diet	205:220	A low-fiber diet has been linked to lower bacterial diversity, obesity, type 2 diabetes, and promotion of mucosal pathogens.
34690938	7	58	theme	published	1014:1022	arg1	growth					1024:1029	published growth and gene expression data	1014:1054	growth	1024:1029	To do so, we compared the existing GH annotation tools and built PUL models by using published growth and gene expression data.
34690938	2	59	theme	mucosal	311:317	arg1	pathogens					319:327	mucosal pathogens	311:327	mucosal pathogens	311:327	A low-fiber diet has been linked to lower bacterial diversity, obesity, type 2 diabetes, and promotion of mucosal pathogens.
34690938	6	60	theme	different	864:872	arg1	fibers					874:879	seven different fibers	858:879	seven different fibers	858:879	We designed an in silico pipeline called FiberGrowth and independently validated it for seven different fibers, on 28 genomes from Bacteroidetes-type strains.
34690938	10	61	theme	further	1536:1542	arg1	experiment					1560:1569	further gene expression experiment	1536:1569	further gene expression experiment	1536:1569	Assuming further gene expression experiment along with discoveries on structural analysis, we hope computational tools like FiberGrowth will help researchers prioritize and design in vitro experiments.
34690938	1	62	theme	gut	134:136	arg1	health					146:151	gut colonic health	134:151	gut colonic health	134:151	Dietary fibers impact gut colonic health, through the production of short-chain fatty acids.
34690938	4	63	theme	utilization	573:583	arg1	PUL					591:593	PUL	591:593	PUL	591:593	However, the GH involved in glycan breakdown (adhesion, hydrolysis, and fermentation) are organized in polysaccharide utilization loci (PUL) with complex modularity.
34690938	4	63	theme	utilization	573:583	arg1	loci					585:588	polysaccharide utilization loci	558:588	polysaccharide utilization loci (PUL) with complex modularity	558:618	However, the GH involved in glycan breakdown (adhesion, hydrolysis, and fermentation) are organized in polysaccharide utilization loci (PUL) with complex modularity.
34690938	7	64	theme	existing	955:962	arg1	tools					978:982	the existing GH annotation tools	951:982	the existing GH annotation tools	951:982	To do so, we compared the existing GH annotation tools and built PUL models by using published growth and gene expression data.
34690938	8	65	from	performance	1082:1092	arg1	terms					1097:1101	terms	1097:1101	terms of true positive rate (TPR) and false positive rate (FPR)	1097:1159	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	2	66	theme	bacterial	247:255	arg1	diversity					257:265	lower bacterial diversity	241:265	lower bacterial diversity	241:265	A low-fiber diet has been linked to lower bacterial diversity, obesity, type 2 diabetes, and promotion of mucosal pathogens.
34690938	8	67	dep	depended	1170:1177	arg1	levan					1319:1323	levan	1319:1323	levan (0.3 and 0.06)	1319:1338	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	8	67	dep	depended	1170:1177	arg1	0.41					1385:1388	0.41	1385:1388	0.41	1385:1388	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	8	67	dep	depended	1170:1177	arg1	0.95					1253:1256	0.95	1253:1256	0.95	1253:1256	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	8	67	dep	depended	1170:1177	arg1	arabinoxylan					1208:1219	arabinoxylan	1208:1219	arabinoxylan (TPR: 0.89 and FPR: 0)	1208:1242	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	8	67	dep	depended	1170:1177	arg1	mucus					1341:1345	mucus	1341:1345	mucus (0.13 and 0.38)	1341:1361	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	8	67	dep	depended	1170:1177	arg1	0.38					1303:1306	0.38	1303:1306	0.38	1303:1306	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	8	67	dep	depended	1170:1177	arg1	starch					1368:1373	starch	1368:1373	starch (0.73 and 0.41)	1368:1389	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	8	67	dep	depended	1170:1177	arg1	0.17					1312:1315	0.17	1312:1315	0.17	1312:1315	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	8	67	dep	depended	1170:1177	arg1	0.33					1262:1265	0.33	1262:1265	0.33	1262:1265	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	8	67	dep	depended	1170:1177	arg1	TPR					1222:1224	TPR	1222:1224	TPR: 0.89 and FPR: 0	1222:1241	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	8	67	dep	depended	1170:1177	arg1	0.73					1376:1379	0.73	1376:1379	0.73	1376:1379	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	8	67	dep	depended	1170:1177	arg1	laminarin					1292:1300	heparin (0.8 and 0.22) laminarin	1269:1300	heparin (0.8 and 0.22) laminarin (0.38 and 0.17)	1269:1316	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	8	67	dep	depended	1170:1177	arg1	inulin					1245:1250	inulin	1245:1250	inulin (0.95 and 0.33)	1245:1266	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	1	68	theme	colonic	138:144	arg1	health					146:151	gut colonic health	134:151	gut colonic health	134:151	Dietary fibers impact gut colonic health, through the production of short-chain fatty acids.
34690938	4	69	theme	polysaccharide	558:571	arg1	PUL					591:593	PUL	591:593	PUL	591:593	However, the GH involved in glycan breakdown (adhesion, hydrolysis, and fermentation) are organized in polysaccharide utilization loci (PUL) with complex modularity.
34690938	4	69	theme	polysaccharide	558:571	arg1	loci					585:588	polysaccharide utilization loci	558:588	polysaccharide utilization loci (PUL) with complex modularity	558:618	However, the GH involved in glycan breakdown (adhesion, hydrolysis, and fermentation) are organized in polysaccharide utilization loci (PUL) with complex modularity.
34690938	2	70	theme	lower	241:245	arg1	diversity					257:265	lower bacterial diversity	241:265	lower bacterial diversity	241:265	A low-fiber diet has been linked to lower bacterial diversity, obesity, type 2 diabetes, and promotion of mucosal pathogens.
34690938	3	71	theme	fatty	442:446	arg1	acids					448:452	short-chain fatty acids	430:452	short-chain fatty acids	430:452	Glycoside hydrolases (GHs) are important enzymes involved in the bacterial catabolism of fiber into short-chain fatty acids.
34690938	8	72	theme	false	1135:1139	arg1	rate					1150:1153	false positive rate	1135:1153	false positive rate (FPR)	1135:1159	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	8	72	theme	false	1135:1139	arg1	FPR					1156:1158	FPR	1156:1158	FPR	1156:1158	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	8	73	dep	levan	1319:1323	arg1	0.06					1334:1337	0.06	1334:1337	0.06	1334:1337	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	8	73	dep	levan	1319:1323	arg1	0.3					1326:1328	0.3	1326:1328	0.3	1326:1328	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	10	74	from	discoveries	1582:1592	arg1	analysis					1608:1615	structural analysis	1597:1615	structural analysis	1597:1615	Assuming further gene expression experiment along with discoveries on structural analysis, we hope computational tools like FiberGrowth will help researchers prioritize and design in vitro experiments.
34690938	8	75	theme	positive	1111:1118	arg1	TPR					1126:1128	TPR	1126:1128	TPR	1126:1128	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	8	75	theme	positive	1111:1118	arg1	rate					1120:1123	true positive rate	1106:1123	true positive rate (TPR)	1106:1129	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
34690938	8	76	theme	prediction	1071:1080	arg1	performance					1082:1092	FiberGrowth's prediction performance	1057:1092	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR)	1057:1159	FiberGrowth's prediction performance in terms of true positive rate (TPR) and false positive rate (FPR) strongly depended on available data and fiber: arabinoxylan (TPR: 0.89 and FPR: 0), inulin (0.95 and 0.33), heparin (0.8 and 0.22) laminarin (0.38 and 0.17), levan (0.3 and 0.06), mucus (0.13 and 0.38), and starch (0.73 and 0.41).
32900799	9	0	from	fermentation	1799:1810	arg1	gut					1819:1821	the gut	1815:1821	the gut	1815:1821	Metabolic processes that are relatively prevalent in the microbiome, such as H2 production, will depend more on substrate, while rare metabolisms such as methanogenesis depend more strongly on microbiome composition.IMPORTANCE Prebiotic fermentation in the gut often leads to the coproduction of short-chain fatty acids (SCFAs) and gases.
32900799	5	1	from	restricted	879:888	arg1	space					901:905	product space	893:905	product space	893:905	Modeling shows that pectin is more restricted in product space, with less potential for H2 but more potential for CO2 production.
32900799	11	2	theme	gut	2261:2263	arg1	microbiota					2265:2274	the human gut microbiota	2251:2274	the human gut microbiota	2251:2274	In this study, we combined the use of theoretical models and an ex vivo experimental platform to illustrate that both the chemical composition of the prebiotic and the community composition of the human gut microbiota can affect the volume and content of gas production during prebiotic fermentation.
32900799	1	3	theme	short-chain	242:252	arg1	SCFAs					267:271	SCFAs	267:271	SCFAs	267:271	Prebiotics confer benefits to human health, often by promoting the growth of gut bacteria that produce metabolites valuable to the human body, such as short-chain fatty acids (SCFAs).
32900799	1	3	theme	short-chain	242:252	arg1	acids					260:264	short-chain fatty acids	242:264	short-chain fatty acids (SCFAs)	242:272	Prebiotics confer benefits to human health, often by promoting the growth of gut bacteria that produce metabolites valuable to the human body, such as short-chain fatty acids (SCFAs).
32900799	12	4	theme	Methanobacteria	2651:2665	arg1	presence					2639:2646	the presence	2635:2646	the presence of Methanobacteria in the microbiota	2635:2683	Specifically, more prevalent metabolic processes such as hydrogen production were strongly affected by the oxidation state of the probiotic, while rare metabolisms such as methane production were less affected by the chemical nature of the substrate and entirely dependent on the presence of Methanobacteria in the microbiota.
32900799	7	5	theme	inulin	1361:1366	arg1	degradation					1368:1378	inulin degradation	1361:1378	inulin degradation	1361:1378	Microbial community composition also impacted results: methane production was dependent on the presence of Methanobacteria, while interindividual differences in H2 production during inulin degradation were driven by a Lachnospiraceae taxon.
32900799	9	6	theme	short-chain	1858:1868	arg1	SCFAs					1883:1887	SCFAs	1883:1887	SCFAs	1883:1887	Metabolic processes that are relatively prevalent in the microbiome, such as H2 production, will depend more on substrate, while rare metabolisms such as methanogenesis depend more strongly on microbiome composition.IMPORTANCE Prebiotic fermentation in the gut often leads to the coproduction of short-chain fatty acids (SCFAs) and gases.
32900799	9	6	theme	short-chain	1858:1868	arg1	acids					1876:1880	short-chain fatty acids	1858:1880	short-chain fatty acids (SCFAs)	1858:1888	Metabolic processes that are relatively prevalent in the microbiome, such as H2 production, will depend more on substrate, while rare metabolisms such as methanogenesis depend more strongly on microbiome composition.IMPORTANCE Prebiotic fermentation in the gut often leads to the coproduction of short-chain fatty acids (SCFAs) and gases.
32900799	6	7	theme	debris	1171:1176	arg1	fermentation					1149:1160	fermentation	1149:1160	fermentation of fecal debris	1149:1176	An ex vivo experimental system showed pectin degradation produced significantly less H2 than inulin, but CO2 production fell outside the theoretical product range, suggesting fermentation of fecal debris.
32900799	4	8	theme	theoretical	779:789	arg1	ranges					799:804	the theoretical product ranges	775:804	the theoretical product ranges of two prebiotics, inulin and pectin	775:841	We first constructed a linear system model based on mass and electron balance and compared the theoretical product ranges of two prebiotics, inulin and pectin.
32900799	12	9	from	presence	2639:2646	arg1	microbiota					2674:2683	the microbiota	2670:2683	the microbiota	2670:2683	Specifically, more prevalent metabolic processes such as hydrogen production were strongly affected by the oxidation state of the probiotic, while rare metabolisms such as methane production were less affected by the chemical nature of the substrate and entirely dependent on the presence of Methanobacteria in the microbiota.
32900799	9	10	theme	acids	1876:1880	arg1	coproduction					1842:1853	the coproduction	1838:1853	the coproduction of short-chain fatty acids (SCFAs) and gases	1838:1898	Metabolic processes that are relatively prevalent in the microbiome, such as H2 production, will depend more on substrate, while rare metabolisms such as methanogenesis depend more strongly on microbiome composition.IMPORTANCE Prebiotic fermentation in the gut often leads to the coproduction of short-chain fatty acids (SCFAs) and gases.
32900799	10	11	theme	excess	1907:1912	arg1	problem					1948:1954	a potential problem	1936:1954	a potential problem for those with functional gut disorders	1936:1994	While excess gas production can be a potential problem for those with functional gut disorders, gas production is rarely considered during prebiotic design.
32900799	10	11	theme	excess	1907:1912	arg1	production					1918:1927	excess gas production	1907:1927	excess gas production	1907:1927	While excess gas production can be a potential problem for those with functional gut disorders, gas production is rarely considered during prebiotic design.
32900799	1	12	theme	bacteria	172:179	arg1	growth					158:163	the growth	154:163	the growth of gut bacteria that produce metabolites valuable to the human body, such as short-chain fatty acids (SCFAs)	154:272	Prebiotics confer benefits to human health, often by promoting the growth of gut bacteria that produce metabolites valuable to the human body, such as short-chain fatty acids (SCFAs).
32900799	12	13	theme	metabolic	2388:2396	arg1	production					2425:2434	hydrogen production	2416:2434	hydrogen production	2416:2434	Specifically, more prevalent metabolic processes such as hydrogen production were strongly affected by the oxidation state of the probiotic, while rare metabolisms such as methane production were less affected by the chemical nature of the substrate and entirely dependent on the presence of Methanobacteria in the microbiota.
32900799	12	13	theme	metabolic	2388:2396	arg1	processes					2398:2406	more prevalent metabolic processes	2373:2406	more prevalent metabolic processes such as hydrogen production	2373:2434	Specifically, more prevalent metabolic processes such as hydrogen production were strongly affected by the oxidation state of the probiotic, while rare metabolisms such as methane production were less affected by the chemical nature of the substrate and entirely dependent on the presence of Methanobacteria in the microbiota.
32900799	11	14	theme	experimental	2130:2141	arg1	platform					2143:2150	an ex vivo experimental platform	2119:2150	an ex vivo experimental platform	2119:2150	In this study, we combined the use of theoretical models and an ex vivo experimental platform to illustrate that both the chemical composition of the prebiotic and the community composition of the human gut microbiota can affect the volume and content of gas production during prebiotic fermentation.
32900799	9	15	from	microbiome	1619:1628	arg1	prevalent					1602:1610	prevalent	1602:1610	prevalent	1602:1610	Metabolic processes that are relatively prevalent in the microbiome, such as H2 production, will depend more on substrate, while rare metabolisms such as methanogenesis depend more strongly on microbiome composition.IMPORTANCE Prebiotic fermentation in the gut often leads to the coproduction of short-chain fatty acids (SCFAs) and gases.
32900799	12	16	theme	oxidation	2466:2474	arg1	state					2476:2480	the oxidation state	2462:2480	the oxidation state of the probiotic	2462:2497	Specifically, more prevalent metabolic processes such as hydrogen production were strongly affected by the oxidation state of the probiotic, while rare metabolisms such as methane production were less affected by the chemical nature of the substrate and entirely dependent on the presence of Methanobacteria in the microbiota.
32900799	7	17	theme	interindividual	1309:1323	arg1	differences					1325:1335	interindividual differences	1309:1335	interindividual differences in H2 production during inulin degradation	1309:1378	Microbial community composition also impacted results: methane production was dependent on the presence of Methanobacteria, while interindividual differences in H2 production during inulin degradation were driven by a Lachnospiraceae taxon.
32900799	11	18	theme	prebiotic	2208:2216	arg1	composition					2189:2199	the chemical composition	2176:2199	the chemical composition of the prebiotic	2176:2216	In this study, we combined the use of theoretical models and an ex vivo experimental platform to illustrate that both the chemical composition of the prebiotic and the community composition of the human gut microbiota can affect the volume and content of gas production during prebiotic fermentation.
32900799	11	18	theme	prebiotic	2208:2216	arg1	composition					2236:2246	the community composition	2222:2246	the community composition of the human gut microbiota	2222:2274	In this study, we combined the use of theoretical models and an ex vivo experimental platform to illustrate that both the chemical composition of the prebiotic and the community composition of the human gut microbiota can affect the volume and content of gas production during prebiotic fermentation.
32900799	1	19	theme	valuable	206:213	arg1	metabolites					194:204	metabolites	194:204	metabolites valuable to the human body	194:231	Prebiotics confer benefits to human health, often by promoting the growth of gut bacteria that produce metabolites valuable to the human body, such as short-chain fatty acids (SCFAs).
32900799	12	20	theme	substrate	2599:2607	arg1	nature					2585:2590	the chemical nature	2572:2590	the chemical nature of the substrate	2572:2607	Specifically, more prevalent metabolic processes such as hydrogen production were strongly affected by the oxidation state of the probiotic, while rare metabolisms such as methane production were less affected by the chemical nature of the substrate and entirely dependent on the presence of Methanobacteria in the microbiota.
32900799	1	21	theme	human	121:125	arg1	health					127:132	human health	121:132	human health	121:132	Prebiotics confer benefits to human health, often by promoting the growth of gut bacteria that produce metabolites valuable to the human body, such as short-chain fatty acids (SCFAs).
32900799	6	22	theme	product	1123:1129	arg1	range					1131:1135	the theoretical product range	1107:1135	the theoretical product range	1107:1135	An ex vivo experimental system showed pectin degradation produced significantly less H2 than inulin, but CO2 production fell outside the theoretical product range, suggesting fermentation of fecal debris.
32900799	11	23	theme	prebiotic	2335:2343	arg1	fermentation					2345:2356	prebiotic fermentation	2335:2356	prebiotic fermentation	2335:2356	In this study, we combined the use of theoretical models and an ex vivo experimental platform to illustrate that both the chemical composition of the prebiotic and the community composition of the human gut microbiota can affect the volume and content of gas production during prebiotic fermentation.
32900799	5	24	theme	CO2	958:960	arg1	production					962:971	CO2 production	958:971	CO2 production	958:971	Modeling shows that pectin is more restricted in product space, with less potential for H2 but more potential for CO2 production.
32900799	3	25	theme	human	556:560	arg1	microbiota					566:575	human gut microbiota	556:575	human gut microbiota	556:575	Here, we investigate how the content and volume of gas production by human gut microbiota are affected by the chemical composition of the prebiotic and the community composition of the microbiota.
32900799	2	26	theme	physiological	446:458	arg1	effects					460:466	physiological effects	446:466	physiological effects	446:466	While prebiotic selection has strongly focused on maximizing the production of SCFAs, less attention has been paid to gases, a by-product of SCFA production that also has physiological effects on the human body.
32900799	10	27	theme	potential	1938:1946	arg1	problem					1948:1954	a potential problem	1936:1954	a potential problem for those with functional gut disorders	1936:1994	While excess gas production can be a potential problem for those with functional gut disorders, gas production is rarely considered during prebiotic design.
32900799	10	27	theme	potential	1938:1946	arg1	production					1918:1927	excess gas production	1907:1927	excess gas production	1907:1927	While excess gas production can be a potential problem for those with functional gut disorders, gas production is rarely considered during prebiotic design.
32900799	3	28	theme	production	542:551	arg1	volume					528:533	volume	528:533	volume	528:533	Here, we investigate how the content and volume of gas production by human gut microbiota are affected by the chemical composition of the prebiotic and the community composition of the microbiota.
32900799	3	28	theme	production	542:551	arg1	content					516:522	content	516:522	content	516:522	Here, we investigate how the content and volume of gas production by human gut microbiota are affected by the chemical composition of the prebiotic and the community composition of the microbiota.
32900799	11	29	theme	ex	2122:2123	arg1	platform					2143:2150	an ex vivo experimental platform	2119:2150	an ex vivo experimental platform	2119:2150	In this study, we combined the use of theoretical models and an ex vivo experimental platform to illustrate that both the chemical composition of the prebiotic and the community composition of the human gut microbiota can affect the volume and content of gas production during prebiotic fermentation.
32900799	10	30	theme	gut	1982:1984	arg1	disorders					1986:1994	functional gut disorders	1971:1994	functional gut disorders	1971:1994	While excess gas production can be a potential problem for those with functional gut disorders, gas production is rarely considered during prebiotic design.
32900799	0	31	theme	Gut	75:77	arg1	Microbiota					79:88	the Human Gut Microbiota	65:88	the Human Gut Microbiota	65:88	Prebiotics and Community Composition Influence Gas Production of the Human Gut Microbiota.
32900799	11	32	theme	chemical	2180:2187	arg1	composition					2189:2199	the chemical composition	2176:2199	the chemical composition of the prebiotic	2176:2216	In this study, we combined the use of theoretical models and an ex vivo experimental platform to illustrate that both the chemical composition of the prebiotic and the community composition of the human gut microbiota can affect the volume and content of gas production during prebiotic fermentation.
32900799	2	33	theme	human	475:479	arg1	body					481:484	the human body	471:484	the human body	471:484	While prebiotic selection has strongly focused on maximizing the production of SCFAs, less attention has been paid to gases, a by-product of SCFA production that also has physiological effects on the human body.
32900799	7	34	theme	Methanobacteria	1286:1300	arg1	presence					1274:1281	the presence	1270:1281	the presence of Methanobacteria	1270:1300	Microbial community composition also impacted results: methane production was dependent on the presence of Methanobacteria, while interindividual differences in H2 production during inulin degradation were driven by a Lachnospiraceae taxon.
32900799	12	35	theme	methane	2531:2537	arg1	production					2539:2548	methane production	2531:2548	methane production	2531:2548	Specifically, more prevalent metabolic processes such as hydrogen production were strongly affected by the oxidation state of the probiotic, while rare metabolisms such as methane production were less affected by the chemical nature of the substrate and entirely dependent on the presence of Methanobacteria in the microbiota.
32900799	4	36	theme	linear	707:712	arg1	model					721:725	a linear system model	705:725	a linear system model based on mass and electron balance	705:760	We first constructed a linear system model based on mass and electron balance and compared the theoretical product ranges of two prebiotics, inulin and pectin.
32900799	9	37	theme	microbiome	1755:1764	arg1	fermentation					1799:1810	microbiome composition.IMPORTANCE Prebiotic fermentation	1755:1810	microbiome composition.IMPORTANCE Prebiotic fermentation in the gut	1755:1821	Metabolic processes that are relatively prevalent in the microbiome, such as H2 production, will depend more on substrate, while rare metabolisms such as methanogenesis depend more strongly on microbiome composition.IMPORTANCE Prebiotic fermentation in the gut often leads to the coproduction of short-chain fatty acids (SCFAs) and gases.
32900799	6	38	theme	ex	977:978	arg1	system					998:1003	An ex vivo experimental system	974:1003	An ex vivo experimental system	974:1003	An ex vivo experimental system showed pectin degradation produced significantly less H2 than inulin, but CO2 production fell outside the theoretical product range, suggesting fermentation of fecal debris.
32900799	11	39	theme	production	2317:2326	arg1	volume					2291:2296	the volume	2287:2296	the volume	2287:2296	In this study, we combined the use of theoretical models and an ex vivo experimental platform to illustrate that both the chemical composition of the prebiotic and the community composition of the human gut microbiota can affect the volume and content of gas production during prebiotic fermentation.
32900799	11	39	theme	production	2317:2326	arg1	content					2302:2308	content	2302:2308	content	2302:2308	In this study, we combined the use of theoretical models and an ex vivo experimental platform to illustrate that both the chemical composition of the prebiotic and the community composition of the human gut microbiota can affect the volume and content of gas production during prebiotic fermentation.
32900799	2	40	theme	SCFAs	354:358	arg1	production					340:349	the production	336:349	the production of SCFAs	336:358	While prebiotic selection has strongly focused on maximizing the production of SCFAs, less attention has been paid to gases, a by-product of SCFA production that also has physiological effects on the human body.
32900799	1	41	theme	fatty	254:258	arg1	SCFAs					267:271	SCFAs	267:271	SCFAs	267:271	Prebiotics confer benefits to human health, often by promoting the growth of gut bacteria that produce metabolites valuable to the human body, such as short-chain fatty acids (SCFAs).
32900799	1	41	theme	fatty	254:258	arg1	acids					260:264	short-chain fatty acids	242:264	short-chain fatty acids (SCFAs)	242:272	Prebiotics confer benefits to human health, often by promoting the growth of gut bacteria that produce metabolites valuable to the human body, such as short-chain fatty acids (SCFAs).
32900799	8	42	theme	prebiotic	1482:1490	arg1	chemistry					1465:1473	the chemistry	1461:1473	the chemistry of the prebiotic	1461:1490	Overall, these results suggest that both the chemistry of the prebiotic and the composition of the microbiota are relevant to gas production.
32900799	8	42	theme	prebiotic	1482:1490	arg1	relevant					1534:1541	relevant	1534:1541	relevant	1534:1541	Overall, these results suggest that both the chemistry of the prebiotic and the composition of the microbiota are relevant to gas production.
32900799	8	42	theme	prebiotic	1482:1490	arg1	composition					1500:1510	the composition	1496:1510	the composition of the microbiota	1496:1528	Overall, these results suggest that both the chemistry of the prebiotic and the composition of the microbiota are relevant to gas production.
32900799	11	43	dep	ex	2122:2123	arg1	vivo					2125:2128	vivo	2125:2128	vivo	2125:2128	In this study, we combined the use of theoretical models and an ex vivo experimental platform to illustrate that both the chemical composition of the prebiotic and the community composition of the human gut microbiota can affect the volume and content of gas production during prebiotic fermentation.
32900799	12	44	theme	rare	2506:2509	arg1	metabolisms					2511:2521	rare metabolisms	2506:2521	rare metabolisms such as methane production	2506:2548	Specifically, more prevalent metabolic processes such as hydrogen production were strongly affected by the oxidation state of the probiotic, while rare metabolisms such as methane production were less affected by the chemical nature of the substrate and entirely dependent on the presence of Methanobacteria in the microbiota.
32900799	12	44	theme	rare	2506:2509	arg1	production					2539:2548	methane production	2531:2548	methane production	2531:2548	Specifically, more prevalent metabolic processes such as hydrogen production were strongly affected by the oxidation state of the probiotic, while rare metabolisms such as methane production were less affected by the chemical nature of the substrate and entirely dependent on the presence of Methanobacteria in the microbiota.
32900799	0	45	theme	Community	15:23	arg1	Composition					25:35	Community Composition	15:35	Community Composition	15:35	Prebiotics and Community Composition Influence Gas Production of the Human Gut Microbiota.
32900799	6	46	theme	CO2	1079:1081	arg1	production					1083:1092	CO2 production	1079:1092	CO2 production	1079:1092	An ex vivo experimental system showed pectin degradation produced significantly less H2 than inulin, but CO2 production fell outside the theoretical product range, suggesting fermentation of fecal debris.
32900799	7	47	theme	methane	1234:1240	arg1	production					1242:1251	methane production	1234:1251	methane production	1234:1251	Microbial community composition also impacted results: methane production was dependent on the presence of Methanobacteria, while interindividual differences in H2 production during inulin degradation were driven by a Lachnospiraceae taxon.
32900799	2	48	theme	production	421:430	arg1	gases					393:397	gases	393:397	gases	393:397	While prebiotic selection has strongly focused on maximizing the production of SCFAs, less attention has been paid to gases, a by-product of SCFA production that also has physiological effects on the human body.
32900799	2	48	theme	production	421:430	arg1	by-product					402:411	a by-product	400:411	a by-product of SCFA production that also has physiological effects on the human body	400:484	While prebiotic selection has strongly focused on maximizing the production of SCFAs, less attention has been paid to gases, a by-product of SCFA production that also has physiological effects on the human body.
32900799	12	49	theme	hydrogen	2416:2423	arg1	production					2425:2434	hydrogen production	2416:2434	hydrogen production	2416:2434	Specifically, more prevalent metabolic processes such as hydrogen production were strongly affected by the oxidation state of the probiotic, while rare metabolisms such as methane production were less affected by the chemical nature of the substrate and entirely dependent on the presence of Methanobacteria in the microbiota.
32900799	9	50	dep	such	1631:1634	arg1	as					1636:1637	as	1636:1637	as	1636:1637	Metabolic processes that are relatively prevalent in the microbiome, such as H2 production, will depend more on substrate, while rare metabolisms such as methanogenesis depend more strongly on microbiome composition.IMPORTANCE Prebiotic fermentation in the gut often leads to the coproduction of short-chain fatty acids (SCFAs) and gases.
32900799	9	51	theme	composition.IMPORTANCE	1766:1787	arg1	fermentation					1799:1810	microbiome composition.IMPORTANCE Prebiotic fermentation	1755:1810	microbiome composition.IMPORTANCE Prebiotic fermentation in the gut	1755:1821	Metabolic processes that are relatively prevalent in the microbiome, such as H2 production, will depend more on substrate, while rare metabolisms such as methanogenesis depend more strongly on microbiome composition.IMPORTANCE Prebiotic fermentation in the gut often leads to the coproduction of short-chain fatty acids (SCFAs) and gases.
32900799	9	52	theme	Prebiotic	1789:1797	arg1	fermentation					1799:1810	microbiome composition.IMPORTANCE Prebiotic fermentation	1755:1810	microbiome composition.IMPORTANCE Prebiotic fermentation in the gut	1755:1821	Metabolic processes that are relatively prevalent in the microbiome, such as H2 production, will depend more on substrate, while rare metabolisms such as methanogenesis depend more strongly on microbiome composition.IMPORTANCE Prebiotic fermentation in the gut often leads to the coproduction of short-chain fatty acids (SCFAs) and gases.
32900799	5	53	theme	more	939:942	arg1	potential					944:952	less potential for H2 but more potential	913:952	less potential for H2 but more potential for CO2 production	913:971	Modeling shows that pectin is more restricted in product space, with less potential for H2 but more potential for CO2 production.
32900799	8	54	theme	microbiota	1519:1528	arg1	chemistry					1465:1473	the chemistry	1461:1473	the chemistry of the prebiotic	1461:1490	Overall, these results suggest that both the chemistry of the prebiotic and the composition of the microbiota are relevant to gas production.
32900799	8	54	theme	microbiota	1519:1528	arg1	relevant					1534:1541	relevant	1534:1541	relevant	1534:1541	Overall, these results suggest that both the chemistry of the prebiotic and the composition of the microbiota are relevant to gas production.
32900799	8	54	theme	microbiota	1519:1528	arg1	composition					1500:1510	the composition	1496:1510	the composition of the microbiota	1496:1528	Overall, these results suggest that both the chemistry of the prebiotic and the composition of the microbiota are relevant to gas production.
32900799	9	55	theme	Metabolic	1562:1570	arg1	processes					1572:1580	Metabolic processes	1562:1580	Metabolic processes that are relatively prevalent in the microbiome	1562:1628	Metabolic processes that are relatively prevalent in the microbiome, such as H2 production, will depend more on substrate, while rare metabolisms such as methanogenesis depend more strongly on microbiome composition.IMPORTANCE Prebiotic fermentation in the gut often leads to the coproduction of short-chain fatty acids (SCFAs) and gases.
32900799	7	56	theme	community	1189:1197	arg1	composition					1199:1209	Microbial community composition	1179:1209	Microbial community composition	1179:1209	Microbial community composition also impacted results: methane production was dependent on the presence of Methanobacteria, while interindividual differences in H2 production during inulin degradation were driven by a Lachnospiraceae taxon.
32900799	11	57	theme	community	2226:2234	arg1	composition					2236:2246	the community composition	2222:2246	the community composition of the human gut microbiota	2222:2274	In this study, we combined the use of theoretical models and an ex vivo experimental platform to illustrate that both the chemical composition of the prebiotic and the community composition of the human gut microbiota can affect the volume and content of gas production during prebiotic fermentation.
32900799	1	58	theme	human	222:226	arg1	body					228:231	the human body	218:231	the human body	218:231	Prebiotics confer benefits to human health, often by promoting the growth of gut bacteria that produce metabolites valuable to the human body, such as short-chain fatty acids (SCFAs).
32900799	11	59	theme	theoretical	2096:2106	arg1	models					2108:2113	theoretical models	2096:2113	theoretical models	2096:2113	In this study, we combined the use of theoretical models and an ex vivo experimental platform to illustrate that both the chemical composition of the prebiotic and the community composition of the human gut microbiota can affect the volume and content of gas production during prebiotic fermentation.
32900799	5	60	theme	potential	918:926	arg1	potential					944:952	less potential for H2 but more potential	913:952	less potential for H2 but more potential for CO2 production	913:971	Modeling shows that pectin is more restricted in product space, with less potential for H2 but more potential for CO2 production.
32900799	11	61	theme	microbiota	2265:2274	arg1	composition					2189:2199	the chemical composition	2176:2199	the chemical composition of the prebiotic	2176:2216	In this study, we combined the use of theoretical models and an ex vivo experimental platform to illustrate that both the chemical composition of the prebiotic and the community composition of the human gut microbiota can affect the volume and content of gas production during prebiotic fermentation.
32900799	11	61	theme	microbiota	2265:2274	arg1	composition					2236:2246	the community composition	2222:2246	the community composition of the human gut microbiota	2222:2274	In this study, we combined the use of theoretical models and an ex vivo experimental platform to illustrate that both the chemical composition of the prebiotic and the community composition of the human gut microbiota can affect the volume and content of gas production during prebiotic fermentation.
32900799	8	62	theme	gas	1546:1548	arg1	production					1550:1559	gas production	1546:1559	gas production	1546:1559	Overall, these results suggest that both the chemistry of the prebiotic and the composition of the microbiota are relevant to gas production.
32900799	5	63	from	space	901:905	arg1	restricted					879:888	restricted	879:888	restricted	879:888	Modeling shows that pectin is more restricted in product space, with less potential for H2 but more potential for CO2 production.
32900799	4	64	theme	product	791:797	arg1	ranges					799:804	the theoretical product ranges	775:804	the theoretical product ranges of two prebiotics, inulin and pectin	775:841	We first constructed a linear system model based on mass and electron balance and compared the theoretical product ranges of two prebiotics, inulin and pectin.
32900799	6	65	theme	experimental	985:996	arg1	system					998:1003	An ex vivo experimental system	974:1003	An ex vivo experimental system	974:1003	An ex vivo experimental system showed pectin degradation produced significantly less H2 than inulin, but CO2 production fell outside the theoretical product range, suggesting fermentation of fecal debris.
32900799	11	66	theme	human	2255:2259	arg1	microbiota					2265:2274	the human gut microbiota	2251:2274	the human gut microbiota	2251:2274	In this study, we combined the use of theoretical models and an ex vivo experimental platform to illustrate that both the chemical composition of the prebiotic and the community composition of the human gut microbiota can affect the volume and content of gas production during prebiotic fermentation.
32900799	7	67	theme	Lachnospiraceae	1397:1411	arg1	taxon					1413:1417	a Lachnospiraceae taxon	1395:1417	a Lachnospiraceae taxon	1395:1417	Microbial community composition also impacted results: methane production was dependent on the presence of Methanobacteria, while interindividual differences in H2 production during inulin degradation were driven by a Lachnospiraceae taxon.
32900799	10	68	theme	prebiotic	2040:2048	arg1	design					2050:2055	prebiotic design	2040:2055	prebiotic design	2040:2055	While excess gas production can be a potential problem for those with functional gut disorders, gas production is rarely considered during prebiotic design.
32900799	3	69	dep	content	516:522	arg1	the					512:514	the	512:514	the	512:514	Here, we investigate how the content and volume of gas production by human gut microbiota are affected by the chemical composition of the prebiotic and the community composition of the microbiota.
32900799	9	70	theme	fatty	1870:1874	arg1	SCFAs					1883:1887	SCFAs	1883:1887	SCFAs	1883:1887	Metabolic processes that are relatively prevalent in the microbiome, such as H2 production, will depend more on substrate, while rare metabolisms such as methanogenesis depend more strongly on microbiome composition.IMPORTANCE Prebiotic fermentation in the gut often leads to the coproduction of short-chain fatty acids (SCFAs) and gases.
32900799	9	70	theme	fatty	1870:1874	arg1	acids					1876:1880	short-chain fatty acids	1858:1880	short-chain fatty acids (SCFAs)	1858:1888	Metabolic processes that are relatively prevalent in the microbiome, such as H2 production, will depend more on substrate, while rare metabolisms such as methanogenesis depend more strongly on microbiome composition.IMPORTANCE Prebiotic fermentation in the gut often leads to the coproduction of short-chain fatty acids (SCFAs) and gases.
32900799	3	71	theme	community	643:651	arg1	composition					653:663	the community composition	639:663	the community composition of the microbiota	639:681	Here, we investigate how the content and volume of gas production by human gut microbiota are affected by the chemical composition of the prebiotic and the community composition of the microbiota.
32900799	6	72	theme	fecal	1165:1169	arg1	debris					1171:1176	fecal debris	1165:1176	fecal debris	1165:1176	An ex vivo experimental system showed pectin degradation produced significantly less H2 than inulin, but CO2 production fell outside the theoretical product range, suggesting fermentation of fecal debris.
32900799	2	73	theme	prebiotic	281:289	arg1	selection					291:299	prebiotic selection	281:299	prebiotic selection	281:299	While prebiotic selection has strongly focused on maximizing the production of SCFAs, less attention has been paid to gases, a by-product of SCFA production that also has physiological effects on the human body.
32900799	1	74	theme	gut	168:170	arg1	bacteria					172:179	gut bacteria	168:179	gut bacteria	168:179	Prebiotics confer benefits to human health, often by promoting the growth of gut bacteria that produce metabolites valuable to the human body, such as short-chain fatty acids (SCFAs).
32900799	1	74	theme	gut	168:170	arg1	acids					260:264	short-chain fatty acids	242:264	short-chain fatty acids (SCFAs)	242:272	Prebiotics confer benefits to human health, often by promoting the growth of gut bacteria that produce metabolites valuable to the human body, such as short-chain fatty acids (SCFAs).
32900799	4	75	theme	electron	745:752	arg1	balance					754:760	mass and electron balance	736:760	balance	754:760	We first constructed a linear system model based on mass and electron balance and compared the theoretical product ranges of two prebiotics, inulin and pectin.
32900799	9	76	theme	H2	1639:1640	arg1	production					1642:1651	H2 production	1639:1651	H2 production	1639:1651	Metabolic processes that are relatively prevalent in the microbiome, such as H2 production, will depend more on substrate, while rare metabolisms such as methanogenesis depend more strongly on microbiome composition.IMPORTANCE Prebiotic fermentation in the gut often leads to the coproduction of short-chain fatty acids (SCFAs) and gases.
32900799	7	77	theme	H2	1340:1341	arg1	production					1343:1352	H2 production	1340:1352	H2 production during inulin degradation	1340:1378	Microbial community composition also impacted results: methane production was dependent on the presence of Methanobacteria, while interindividual differences in H2 production during inulin degradation were driven by a Lachnospiraceae taxon.
32900799	3	78	theme	microbiota	672:681	arg1	composition					653:663	the community composition	639:663	the community composition of the microbiota	639:681	Here, we investigate how the content and volume of gas production by human gut microbiota are affected by the chemical composition of the prebiotic and the community composition of the microbiota.
32900799	3	78	theme	microbiota	672:681	arg1	composition					606:616	the chemical composition	593:616	the chemical composition of the prebiotic	593:633	Here, we investigate how the content and volume of gas production by human gut microbiota are affected by the chemical composition of the prebiotic and the community composition of the microbiota.
32900799	9	79	from	prevalent	1602:1610	arg1	microbiome					1619:1628	the microbiome	1615:1628	the microbiome	1615:1628	Metabolic processes that are relatively prevalent in the microbiome, such as H2 production, will depend more on substrate, while rare metabolisms such as methanogenesis depend more strongly on microbiome composition.IMPORTANCE Prebiotic fermentation in the gut often leads to the coproduction of short-chain fatty acids (SCFAs) and gases.
32900799	9	80	theme	gases	1894:1898	arg1	coproduction					1842:1853	the coproduction	1838:1853	the coproduction of short-chain fatty acids (SCFAs) and gases	1838:1898	Metabolic processes that are relatively prevalent in the microbiome, such as H2 production, will depend more on substrate, while rare metabolisms such as methanogenesis depend more strongly on microbiome composition.IMPORTANCE Prebiotic fermentation in the gut often leads to the coproduction of short-chain fatty acids (SCFAs) and gases.
32900799	5	81	with	restricted	879:888	arg1	potential					944:952	less potential for H2 but more potential	913:952	less potential for H2 but more potential for CO2 production	913:971	Modeling shows that pectin is more restricted in product space, with less potential for H2 but more potential for CO2 production.
32900799	4	82	theme	prebiotics	813:822	arg1	ranges					799:804	the theoretical product ranges	775:804	the theoretical product ranges of two prebiotics, inulin and pectin	775:841	We first constructed a linear system model based on mass and electron balance and compared the theoretical product ranges of two prebiotics, inulin and pectin.
32900799	10	83	theme	gas	1914:1916	arg1	problem					1948:1954	a potential problem	1936:1954	a potential problem for those with functional gut disorders	1936:1994	While excess gas production can be a potential problem for those with functional gut disorders, gas production is rarely considered during prebiotic design.
32900799	10	83	theme	gas	1914:1916	arg1	production					1918:1927	excess gas production	1907:1927	excess gas production	1907:1927	While excess gas production can be a potential problem for those with functional gut disorders, gas production is rarely considered during prebiotic design.
32900799	6	84	theme	theoretical	1111:1121	arg1	range					1131:1135	the theoretical product range	1107:1135	the theoretical product range	1107:1135	An ex vivo experimental system showed pectin degradation produced significantly less H2 than inulin, but CO2 production fell outside the theoretical product range, suggesting fermentation of fecal debris.
32900799	12	85	attach	presence	2639:2646	arg1	microbiota					2674:2683	the microbiota	2670:2683	the microbiota	2670:2683	Specifically, more prevalent metabolic processes such as hydrogen production were strongly affected by the oxidation state of the probiotic, while rare metabolisms such as methane production were less affected by the chemical nature of the substrate and entirely dependent on the presence of Methanobacteria in the microbiota.
32900799	12	85	attach	presence	2639:2646	arg2	Methanobacteria					2651:2665	Methanobacteria	2651:2665	Methanobacteria	2651:2665	Specifically, more prevalent metabolic processes such as hydrogen production were strongly affected by the oxidation state of the probiotic, while rare metabolisms such as methane production were less affected by the chemical nature of the substrate and entirely dependent on the presence of Methanobacteria in the microbiota.
32900799	0	86	theme	Human	69:73	arg1	Microbiota					79:88	the Human Gut Microbiota	65:88	the Human Gut Microbiota	65:88	Prebiotics and Community Composition Influence Gas Production of the Human Gut Microbiota.
32900799	3	87	theme	gas	538:540	arg1	production					542:551	gas production	538:551	gas production	538:551	Here, we investigate how the content and volume of gas production by human gut microbiota are affected by the chemical composition of the prebiotic and the community composition of the microbiota.
32900799	0	88	theme	Microbiota	79:88	arg1	Production					51:60	Gas Production	47:60	Gas Production of the Human Gut Microbiota	47:88	Prebiotics and Community Composition Influence Gas Production of the Human Gut Microbiota.
32900799	9	89	theme	rare	1691:1694	arg1	metabolisms					1696:1706	rare metabolisms	1691:1706	rare metabolisms such as methanogenesis	1691:1729	Metabolic processes that are relatively prevalent in the microbiome, such as H2 production, will depend more on substrate, while rare metabolisms such as methanogenesis depend more strongly on microbiome composition.IMPORTANCE Prebiotic fermentation in the gut often leads to the coproduction of short-chain fatty acids (SCFAs) and gases.
32900799	9	89	theme	rare	1691:1694	arg1	methanogenesis					1716:1729	methanogenesis	1716:1729	methanogenesis	1716:1729	Metabolic processes that are relatively prevalent in the microbiome, such as H2 production, will depend more on substrate, while rare metabolisms such as methanogenesis depend more strongly on microbiome composition.IMPORTANCE Prebiotic fermentation in the gut often leads to the coproduction of short-chain fatty acids (SCFAs) and gases.
32900799	10	90	theme	functional	1971:1980	arg1	disorders					1986:1994	functional gut disorders	1971:1994	functional gut disorders	1971:1994	While excess gas production can be a potential problem for those with functional gut disorders, gas production is rarely considered during prebiotic design.
32900799	5	91	theme	product	893:899	arg1	space					901:905	product space	893:905	product space	893:905	Modeling shows that pectin is more restricted in product space, with less potential for H2 but more potential for CO2 production.
32900799	12	92	theme	probiotic	2489:2497	arg1	state					2476:2480	the oxidation state	2462:2480	the oxidation state of the probiotic	2462:2497	Specifically, more prevalent metabolic processes such as hydrogen production were strongly affected by the oxidation state of the probiotic, while rare metabolisms such as methane production were less affected by the chemical nature of the substrate and entirely dependent on the presence of Methanobacteria in the microbiota.
32900799	11	93	theme	gas	2313:2315	arg1	production					2317:2326	gas production	2313:2326	gas production	2313:2326	In this study, we combined the use of theoretical models and an ex vivo experimental platform to illustrate that both the chemical composition of the prebiotic and the community composition of the human gut microbiota can affect the volume and content of gas production during prebiotic fermentation.
32900799	12	94	theme	prevalent	2378:2386	arg1	production					2425:2434	hydrogen production	2416:2434	hydrogen production	2416:2434	Specifically, more prevalent metabolic processes such as hydrogen production were strongly affected by the oxidation state of the probiotic, while rare metabolisms such as methane production were less affected by the chemical nature of the substrate and entirely dependent on the presence of Methanobacteria in the microbiota.
32900799	12	94	theme	prevalent	2378:2386	arg1	processes					2398:2406	more prevalent metabolic processes	2373:2406	more prevalent metabolic processes such as hydrogen production	2373:2434	Specifically, more prevalent metabolic processes such as hydrogen production were strongly affected by the oxidation state of the probiotic, while rare metabolisms such as methane production were less affected by the chemical nature of the substrate and entirely dependent on the presence of Methanobacteria in the microbiota.
32900799	6	95	dep	ex	977:978	arg1	vivo					980:983	vivo	980:983	vivo	980:983	An ex vivo experimental system showed pectin degradation produced significantly less H2 than inulin, but CO2 production fell outside the theoretical product range, suggesting fermentation of fecal debris.
32900799	0	96	theme	Gas	47:49	arg1	Production					51:60	Gas Production	47:60	Gas Production of the Human Gut Microbiota	47:88	Prebiotics and Community Composition Influence Gas Production of the Human Gut Microbiota.
32900799	7	97	from	differences	1325:1335	arg1	production					1343:1352	H2 production	1340:1352	H2 production during inulin degradation	1340:1378	Microbial community composition also impacted results: methane production was dependent on the presence of Methanobacteria, while interindividual differences in H2 production during inulin degradation were driven by a Lachnospiraceae taxon.
32900799	2	98	theme	SCFA	416:419	arg1	production					421:430	SCFA production	416:430	SCFA production	416:430	While prebiotic selection has strongly focused on maximizing the production of SCFAs, less attention has been paid to gases, a by-product of SCFA production that also has physiological effects on the human body.
32900799	3	99	theme	gut	562:564	arg1	microbiota					566:575	human gut microbiota	556:575	human gut microbiota	556:575	Here, we investigate how the content and volume of gas production by human gut microbiota are affected by the chemical composition of the prebiotic and the community composition of the microbiota.
32900799	4	100	theme	mass	736:739	arg1	balance					754:760	mass and electron balance	736:760	balance	754:760	We first constructed a linear system model based on mass and electron balance and compared the theoretical product ranges of two prebiotics, inulin and pectin.
32900799	6	101	theme	less	1054:1057	arg1	H2					1059:1060	significantly less H2	1040:1060	significantly less H2	1040:1060	An ex vivo experimental system showed pectin degradation produced significantly less H2 than inulin, but CO2 production fell outside the theoretical product range, suggesting fermentation of fecal debris.
32900799	11	102	theme	models	2108:2113	arg1	use					2089:2091	the use	2085:2091	the use of theoretical models	2085:2113	In this study, we combined the use of theoretical models and an ex vivo experimental platform to illustrate that both the chemical composition of the prebiotic and the community composition of the human gut microbiota can affect the volume and content of gas production during prebiotic fermentation.
32900799	11	102	theme	models	2108:2113	arg1	platform					2143:2150	an ex vivo experimental platform	2119:2150	an ex vivo experimental platform	2119:2150	In this study, we combined the use of theoretical models and an ex vivo experimental platform to illustrate that both the chemical composition of the prebiotic and the community composition of the human gut microbiota can affect the volume and content of gas production during prebiotic fermentation.
32900799	3	103	theme	chemical	597:604	arg1	composition					606:616	the chemical composition	593:616	the chemical composition of the prebiotic	593:633	Here, we investigate how the content and volume of gas production by human gut microbiota are affected by the chemical composition of the prebiotic and the community composition of the microbiota.
32900799	6	104	dep	showed	1005:1010	arg1	produced					1031:1038	produced	1031:1038	showed pectin degradation produced significantly less H2 than inulin	1005:1072	An ex vivo experimental system showed pectin degradation produced significantly less H2 than inulin, but CO2 production fell outside the theoretical product range, suggesting fermentation of fecal debris.
32900799	2	105	contain	has	442:444	arg2	effects					460:466	physiological effects	446:466	physiological effects	446:466	While prebiotic selection has strongly focused on maximizing the production of SCFAs, less attention has been paid to gases, a by-product of SCFA production that also has physiological effects on the human body.
32900799	2	105	contain	has	442:444	arg1	by-product					402:411	a by-product	400:411	a by-product of SCFA production that also has physiological effects on the human body	400:484	While prebiotic selection has strongly focused on maximizing the production of SCFAs, less attention has been paid to gases, a by-product of SCFA production that also has physiological effects on the human body.
32900799	2	105	contain	has	442:444	arg1	gases					393:397	gases	393:397	gases	393:397	While prebiotic selection has strongly focused on maximizing the production of SCFAs, less attention has been paid to gases, a by-product of SCFA production that also has physiological effects on the human body.
32900799	7	106	theme	Microbial	1179:1187	arg1	composition					1199:1209	Microbial community composition	1179:1209	Microbial community composition	1179:1209	Microbial community composition also impacted results: methane production was dependent on the presence of Methanobacteria, while interindividual differences in H2 production during inulin degradation were driven by a Lachnospiraceae taxon.
32900799	10	107	theme	gas	1997:1999	arg1	production					2001:2010	gas production	1997:2010	gas production	1997:2010	While excess gas production can be a potential problem for those with functional gut disorders, gas production is rarely considered during prebiotic design.
32900799	12	108	theme	chemical	2576:2583	arg1	nature					2585:2590	the chemical nature	2572:2590	the chemical nature of the substrate	2572:2607	Specifically, more prevalent metabolic processes such as hydrogen production were strongly affected by the oxidation state of the probiotic, while rare metabolisms such as methane production were less affected by the chemical nature of the substrate and entirely dependent on the presence of Methanobacteria in the microbiota.
32900799	4	109	theme	system	714:719	arg1	model					721:725	a linear system model	705:725	a linear system model based on mass and electron balance	705:760	We first constructed a linear system model based on mass and electron balance and compared the theoretical product ranges of two prebiotics, inulin and pectin.
32900799	6	110	theme	pectin	1012:1017	arg1	degradation					1019:1029	pectin degradation	1012:1029	pectin degradation	1012:1029	An ex vivo experimental system showed pectin degradation produced significantly less H2 than inulin, but CO2 production fell outside the theoretical product range, suggesting fermentation of fecal debris.
32900799	3	111	theme	prebiotic	625:633	arg1	composition					653:663	the community composition	639:663	the community composition of the microbiota	639:681	Here, we investigate how the content and volume of gas production by human gut microbiota are affected by the chemical composition of the prebiotic and the community composition of the microbiota.
32900799	3	111	theme	prebiotic	625:633	arg1	composition					606:616	the chemical composition	593:616	the chemical composition of the prebiotic	593:633	Here, we investigate how the content and volume of gas production by human gut microbiota are affected by the chemical composition of the prebiotic and the community composition of the microbiota.
33142610	9	0	theme	biomedical	1141:1150	arg1	field					1152:1156	biomedical field	1141:1156	biomedical field	1141:1156	Overall, the deposition of CS and PDA may further expand the use of SF in biomedical field.
33142610	5	1	theme	tensile	633:639	arg1	modulus					641:647	the tensile modulus	629:647	the tensile modulus of the mats in wet condition	629:676	After depositing 15 bilayers, the tensile modulus of the mats in wet condition increased from 2.16 MPa (pristine SF mats) to 4.89 MPa.
33142610	7	2	theme	improved	878:885	arg1	ability					901:907	improved antibacterial ability	878:907	improved antibacterial ability of more than 98 %	878:925	Besides, LBL structured mats showed improved antibacterial ability of more than 98 % against E. coli and S. aureus.
33142610	8	3	theme	cells	1060:1064	arg1	experiment					1041:1050	the proliferation experiment	1023:1050	the proliferation experiment of L929 cells	1023:1064	In addition, advancement in biocompatibility was observed during the proliferation experiment of L929 cells.
33142610	8	4	theme	proliferation	1027:1039	arg1	experiment					1041:1050	the proliferation experiment	1023:1050	the proliferation experiment of L929 cells	1023:1064	In addition, advancement in biocompatibility was observed during the proliferation experiment of L929 cells.
33142610	7	5	theme	structured	855:864	arg1	mats					866:869	LBL structured mats	851:869	LBL structured mats	851:869	Besides, LBL structured mats showed improved antibacterial ability of more than 98 % against E. coli and S. aureus.
33142610	8	6	located	observed	1007:1014	arg2	advancement					971:981	advancement	971:981	advancement in biocompatibility	971:1001	In addition, advancement in biocompatibility was observed during the proliferation experiment of L929 cells.
33142610	8	6	located	observed	1007:1014	arg1	addition					961:968	addition	961:968	addition	961:968	In addition, advancement in biocompatibility was observed during the proliferation experiment of L929 cells.
33142610	8	7	theme	L929	1055:1058	arg1	cells					1060:1064	L929 cells	1055:1064	L929 cells	1055:1064	In addition, advancement in biocompatibility was observed during the proliferation experiment of L929 cells.
33142610	6	8	theme	more	807:810	arg1	bilayers					812:819	more bilayers	807:819	more bilayers coating on the mats	807:839	A trend towards better hydrophilicity performance was also recorded with more bilayers coating on the mats.
33142610	5	9	theme	mats	656:659	arg1	modulus					641:647	the tensile modulus	629:647	the tensile modulus of the mats in wet condition	629:676	After depositing 15 bilayers, the tensile modulus of the mats in wet condition increased from 2.16 MPa (pristine SF mats) to 4.89 MPa.
33142610	0	10	theme	Chitosan/polydopamine	0:20	arg1	layer					22:26	Chitosan/polydopamine layer	0:26	Chitosan/polydopamine layer by layer	0:35	Chitosan/polydopamine layer by layer self-assembled silk fibroin nanofibers for biomedical applications.
33142610	1	11	theme	superior	176:183	arg1	biocompatibility					185:200	its superior biocompatibility	172:200	its superior biocompatibility	172:200	Silk fibroin (SF) is increasingly needed in tissue engineering for its superior biocompatibility.
33142610	3	12	theme	electrospun	375:385	arg1	mats					402:405	electrospun nanofibrous SF mats	375:405	electrospun nanofibrous SF mats	375:405	In this study, chitosan (CS) and polydopamine (PDA) were introduced into electrospun nanofibrous SF mats through layer-by-layer self-assembly (LBL) to obtain enhanced antibacterial ability and cytocompatibility.
33142610	1	13	theme	Silk	105:108	arg1	fibroin					110:116	Silk fibroin	105:116	Silk fibroin (SF)	105:121	Silk fibroin (SF) is increasingly needed in tissue engineering for its superior biocompatibility.
33142610	1	13	theme	Silk	105:108	arg1	SF					119:120	SF	119:120	SF	119:120	Silk fibroin (SF) is increasingly needed in tissue engineering for its superior biocompatibility.
33142610	7	14	theme	LBL	851:853	arg1	mats					866:869	LBL structured mats	851:869	LBL structured mats	851:869	Besides, LBL structured mats showed improved antibacterial ability of more than 98 % against E. coli and S. aureus.
33142610	9	15	from	use	1128:1130	arg1	field					1152:1156	biomedical field	1141:1156	biomedical field	1141:1156	Overall, the deposition of CS and PDA may further expand the use of SF in biomedical field.
33142610	3	16	theme	nanofibrous	387:397	arg1	mats					402:405	electrospun nanofibrous SF mats	375:405	electrospun nanofibrous SF mats	375:405	In this study, chitosan (CS) and polydopamine (PDA) were introduced into electrospun nanofibrous SF mats through layer-by-layer self-assembly (LBL) to obtain enhanced antibacterial ability and cytocompatibility.
33142610	5	17	theme	wet	664:666	arg1	condition					668:676	wet condition	664:676	wet condition	664:676	After depositing 15 bilayers, the tensile modulus of the mats in wet condition increased from 2.16 MPa (pristine SF mats) to 4.89 MPa.
33142610	7	18	theme	%	925:925	arg1	ability					901:907	improved antibacterial ability	878:907	improved antibacterial ability of more than 98 %	878:925	Besides, LBL structured mats showed improved antibacterial ability of more than 98 % against E. coli and S. aureus.
33142610	4	19	theme	successful	576:585	arg1	deposition					587:596	the successful deposition	572:596	the successful deposition	572:596	The surface morphology and composition analysis confirmed the successful deposition.
33142610	9	20	theme	CS	1094:1095	arg1	deposition					1080:1089	the deposition	1076:1089	the deposition of CS and PDA	1076:1103	Overall, the deposition of CS and PDA may further expand the use of SF in biomedical field.
33142610	3	21	theme	enhanced	460:467	arg1	ability					483:489	enhanced antibacterial ability	460:489	enhanced antibacterial ability	460:489	In this study, chitosan (CS) and polydopamine (PDA) were introduced into electrospun nanofibrous SF mats through layer-by-layer self-assembly (LBL) to obtain enhanced antibacterial ability and cytocompatibility.
33142610	5	22	dep	increased	678:686	arg1	mats					715:718	pristine SF mats	703:718	pristine SF mats	703:718	After depositing 15 bilayers, the tensile modulus of the mats in wet condition increased from 2.16 MPa (pristine SF mats) to 4.89 MPa.
33142610	3	23	theme	antibacterial	469:481	arg1	ability					483:489	enhanced antibacterial ability	460:489	enhanced antibacterial ability	460:489	In this study, chitosan (CS) and polydopamine (PDA) were introduced into electrospun nanofibrous SF mats through layer-by-layer self-assembly (LBL) to obtain enhanced antibacterial ability and cytocompatibility.
33142610	0	24	theme	silk	52:55	arg1	nanofibers					65:74	silk fibroin nanofibers	52:74	silk fibroin nanofibers for biomedical applications	52:102	Chitosan/polydopamine layer by layer self-assembled silk fibroin nanofibers for biomedical applications.
33142610	5	25	from	mats	656:659	arg1	condition					668:676	wet condition	664:676	wet condition	664:676	After depositing 15 bilayers, the tensile modulus of the mats in wet condition increased from 2.16 MPa (pristine SF mats) to 4.89 MPa.
33142610	2	26	theme	SF	247:248	arg1	biomaterials					250:261	pure SF biomaterials	242:261	pure SF biomaterials	242:261	However, the practical applications of pure SF biomaterials confront bacterial infection problems.
33142610	9	27	theme	PDA	1101:1103	arg1	deposition					1080:1089	the deposition	1076:1089	the deposition of CS and PDA	1076:1103	Overall, the deposition of CS and PDA may further expand the use of SF in biomedical field.
33142610	3	28	theme	layer-by-layer	415:428	arg1	LBL					445:447	LBL	445:447	LBL	445:447	In this study, chitosan (CS) and polydopamine (PDA) were introduced into electrospun nanofibrous SF mats through layer-by-layer self-assembly (LBL) to obtain enhanced antibacterial ability and cytocompatibility.
33142610	3	28	theme	layer-by-layer	415:428	arg1	self-assembly					430:442	layer-by-layer self-assembly	415:442	layer-by-layer self-assembly (LBL)	415:448	In this study, chitosan (CS) and polydopamine (PDA) were introduced into electrospun nanofibrous SF mats through layer-by-layer self-assembly (LBL) to obtain enhanced antibacterial ability and cytocompatibility.
33142610	2	29	theme	pure	242:245	arg1	biomaterials					250:261	pure SF biomaterials	242:261	pure SF biomaterials	242:261	However, the practical applications of pure SF biomaterials confront bacterial infection problems.
33142610	3	30	theme	SF	399:400	arg1	mats					402:405	electrospun nanofibrous SF mats	375:405	electrospun nanofibrous SF mats	375:405	In this study, chitosan (CS) and polydopamine (PDA) were introduced into electrospun nanofibrous SF mats through layer-by-layer self-assembly (LBL) to obtain enhanced antibacterial ability and cytocompatibility.
33142610	4	31	theme	composition	541:551	arg1	analysis					553:560	composition analysis	541:560	composition analysis	541:560	The surface morphology and composition analysis confirmed the successful deposition.
33142610	6	32	theme	hydrophilicity	757:770	arg1	performance					772:782	better hydrophilicity performance	750:782	better hydrophilicity performance	750:782	A trend towards better hydrophilicity performance was also recorded with more bilayers coating on the mats.
33142610	8	33	from	advancement	971:981	arg1	biocompatibility					986:1001	biocompatibility	986:1001	biocompatibility	986:1001	In addition, advancement in biocompatibility was observed during the proliferation experiment of L929 cells.
33142610	0	34	theme	fibroin	57:63	arg1	nanofibers					65:74	silk fibroin nanofibers	52:74	silk fibroin nanofibers for biomedical applications	52:102	Chitosan/polydopamine layer by layer self-assembled silk fibroin nanofibers for biomedical applications.
33142610	5	35	from	condition	668:676	arg1	modulus					641:647	the tensile modulus	629:647	the tensile modulus of the mats in wet condition	629:676	After depositing 15 bilayers, the tensile modulus of the mats in wet condition increased from 2.16 MPa (pristine SF mats) to 4.89 MPa.
33142610	2	36	theme	practical	216:224	arg1	applications					226:237	the practical applications	212:237	the practical applications of pure SF biomaterials	212:261	However, the practical applications of pure SF biomaterials confront bacterial infection problems.
33142610	6	37	theme	better	750:755	arg1	performance					772:782	better hydrophilicity performance	750:782	better hydrophilicity performance	750:782	A trend towards better hydrophilicity performance was also recorded with more bilayers coating on the mats.
33142610	5	38	theme	pristine	703:710	arg1	mats					715:718	pristine SF mats	703:718	pristine SF mats	703:718	After depositing 15 bilayers, the tensile modulus of the mats in wet condition increased from 2.16 MPa (pristine SF mats) to 4.89 MPa.
33142610	2	39	theme	infection	282:290	arg1	problems					292:299	bacterial infection problems	272:299	bacterial infection problems	272:299	However, the practical applications of pure SF biomaterials confront bacterial infection problems.
33142610	5	40	theme	SF	712:713	arg1	mats					715:718	pristine SF mats	703:718	pristine SF mats	703:718	After depositing 15 bilayers, the tensile modulus of the mats in wet condition increased from 2.16 MPa (pristine SF mats) to 4.89 MPa.
33142610	2	41	theme	bacterial	272:280	arg1	problems					292:299	bacterial infection problems	272:299	bacterial infection problems	272:299	However, the practical applications of pure SF biomaterials confront bacterial infection problems.
33142610	0	42	theme	biomedical	80:89	arg1	applications					91:102	biomedical applications	80:102	biomedical applications	80:102	Chitosan/polydopamine layer by layer self-assembled silk fibroin nanofibers for biomedical applications.
33142610	5	43	from	modulus	641:647	arg1	condition					668:676	wet condition	664:676	wet condition	664:676	After depositing 15 bilayers, the tensile modulus of the mats in wet condition increased from 2.16 MPa (pristine SF mats) to 4.89 MPa.
33142610	4	44	theme	surface	518:524	arg1	morphology					526:535	surface morphology	518:535	surface morphology	518:535	The surface morphology and composition analysis confirmed the successful deposition.
33142610	2	45	theme	biomaterials	250:261	arg1	applications					226:237	the practical applications	212:237	the practical applications of pure SF biomaterials	212:261	However, the practical applications of pure SF biomaterials confront bacterial infection problems.
33142610	9	46	theme	SF	1135:1136	arg1	use					1128:1130	the use	1124:1130	the use of SF in biomedical field	1124:1156	Overall, the deposition of CS and PDA may further expand the use of SF in biomedical field.
33142610	1	47	theme	tissue	149:154	arg1	engineering					156:166	tissue engineering	149:166	tissue engineering	149:166	Silk fibroin (SF) is increasingly needed in tissue engineering for its superior biocompatibility.
33142610	7	48	theme	antibacterial	887:899	arg1	ability					901:907	improved antibacterial ability	878:907	improved antibacterial ability of more than 98 %	878:925	Besides, LBL structured mats showed improved antibacterial ability of more than 98 % against E. coli and S. aureus.
32470590	2	0	theme	/hyaluronic	338:348	arg1	hydrogels					360:368	collagen type I (Col I)/hyaluronic acid (HA) hydrogels	315:368	collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different chemical crosslinking manners	315:432	In this study, three kinds of collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different chemical crosslinking manners were designed and fabricated, and the effects of chemical crosslinking manners on the physical properties and biocompatibility of hydrogels were investigated.
32470590	2	1	theme	physical	520:527	arg1	properties					529:538	physical properties	520:538	physical properties	520:538	In this study, three kinds of collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different chemical crosslinking manners were designed and fabricated, and the effects of chemical crosslinking manners on the physical properties and biocompatibility of hydrogels were investigated.
32470590	0	2	from	effects	4:10	arg1	biocompatibility					76:91	biocompatibility	76:91	biocompatibility	76:91	The effects of chemical crosslinking manners on the physical properties and biocompatibility of collagen type I/hyaluronic acid composite hydrogels.
32470590	0	2	from	effects	4:10	arg1	properties					61:70	physical properties	52:70	physical properties	52:70	The effects of chemical crosslinking manners on the physical properties and biocompatibility of collagen type I/hyaluronic acid composite hydrogels.
32470590	2	3	dep	properties	529:538	arg1	the					516:518	the	516:518	the	516:518	In this study, three kinds of collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different chemical crosslinking manners were designed and fabricated, and the effects of chemical crosslinking manners on the physical properties and biocompatibility of hydrogels were investigated.
32470590	1	4	theme	hydrogels	274:282	arg1	biocompatibility					254:269	biocompatibility	254:269	biocompatibility	254:269	It is necessary to use chemical crosslinking to regulate the mechanical properties, biodegradability and biocompatibility of hydrogels.
32470590	1	4	theme	hydrogels	274:282	arg1	biodegradability					233:248	biodegradability	233:248	biodegradability	233:248	It is necessary to use chemical crosslinking to regulate the mechanical properties, biodegradability and biocompatibility of hydrogels.
32470590	1	4	theme	hydrogels	274:282	arg1	properties					221:230	the mechanical properties	206:230	the mechanical properties	206:230	It is necessary to use chemical crosslinking to regulate the mechanical properties, biodegradability and biocompatibility of hydrogels.
32470590	0	5	theme	collagen	96:103	arg1	hydrogels					138:146	collagen type I/hyaluronic acid composite hydrogels	96:146	collagen type I/hyaluronic acid composite hydrogels	96:146	The effects of chemical crosslinking manners on the physical properties and biocompatibility of collagen type I/hyaluronic acid composite hydrogels.
32470590	2	6	with	hydrogels	360:368	arg1	manners					426:432	different chemical crosslinking manners	394:432	different chemical crosslinking manners	394:432	In this study, three kinds of collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different chemical crosslinking manners were designed and fabricated, and the effects of chemical crosslinking manners on the physical properties and biocompatibility of hydrogels were investigated.
32470590	2	6	with	hydrogels	360:368	arg1	ratio					384:388	the same ratio	375:388	the same ratio	375:388	In this study, three kinds of collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different chemical crosslinking manners were designed and fabricated, and the effects of chemical crosslinking manners on the physical properties and biocompatibility of hydrogels were investigated.
32470590	9	7	theme	acceptable	1442:1451	arg1	reaction					1466:1473	similar and acceptable inflammatory reaction	1430:1473	similar and acceptable inflammatory reaction	1430:1473	In vivo studies demonstrated that these hydrogels showed similar and acceptable inflammatory reaction.
32470590	4	8	theme	cell	772:775	arg1	survival					777:784	cell survival	772:784	cell survival	772:784	Chondrocytes were encapsulated into these hydrogels to detect their effects on cell survival, proliferation, morphology and ECM secretion.
32470590	6	9	theme	crosslinking	997:1008	arg1	manners					1010:1016	chemical crosslinking manners	988:1016	chemical crosslinking manners of hydrogels	988:1029	The results showed that chemical crosslinking manners of hydrogels could affect their physical properties to some extent.
32470590	2	10	theme	crosslinking	492:503	arg1	manners					505:511	chemical crosslinking manners	483:511	chemical crosslinking manners	483:511	In this study, three kinds of collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different chemical crosslinking manners were designed and fabricated, and the effects of chemical crosslinking manners on the physical properties and biocompatibility of hydrogels were investigated.
32470590	7	11	theme	round	1142:1146	arg1	shape					1156:1160	a round or oval shape	1140:1160	a round or oval shape	1140:1160	Chondrocytes encapsulated into these hydrogels showed a round or oval shape.
32470590	6	12	theme	chemical	988:995	arg1	manners					1010:1016	chemical crosslinking manners	988:1016	chemical crosslinking manners of hydrogels	988:1029	The results showed that chemical crosslinking manners of hydrogels could affect their physical properties to some extent.
32470590	0	13	theme	I/hyaluronic	110:121	arg1	hydrogels					138:146	collagen type I/hyaluronic acid composite hydrogels	96:146	collagen type I/hyaluronic acid composite hydrogels	96:146	The effects of chemical crosslinking manners on the physical properties and biocompatibility of collagen type I/hyaluronic acid composite hydrogels.
32470590	9	14	dep	In	1373:1374	arg1	vivo					1376:1379	vivo	1376:1379	vivo	1376:1379	In vivo studies demonstrated that these hydrogels showed similar and acceptable inflammatory reaction.
32470590	9	15	theme	similar	1430:1436	arg1	reaction					1466:1473	similar and acceptable inflammatory reaction	1430:1473	similar and acceptable inflammatory reaction	1430:1473	In vivo studies demonstrated that these hydrogels showed similar and acceptable inflammatory reaction.
32470590	0	16	theme	type	105:108	arg1	hydrogels					138:146	collagen type I/hyaluronic acid composite hydrogels	96:146	collagen type I/hyaluronic acid composite hydrogels	96:146	The effects of chemical crosslinking manners on the physical properties and biocompatibility of collagen type I/hyaluronic acid composite hydrogels.
32470590	4	17	from	effects	761:767	arg1	morphology					802:811	morphology	802:811	morphology	802:811	Chondrocytes were encapsulated into these hydrogels to detect their effects on cell survival, proliferation, morphology and ECM secretion.
32470590	4	17	from	effects	761:767	arg1	secretion					821:829	ECM secretion	817:829	ECM secretion	817:829	Chondrocytes were encapsulated into these hydrogels to detect their effects on cell survival, proliferation, morphology and ECM secretion.
32470590	4	17	from	effects	761:767	arg1	proliferation					787:799	proliferation	787:799	proliferation	787:799	Chondrocytes were encapsulated into these hydrogels to detect their effects on cell survival, proliferation, morphology and ECM secretion.
32470590	4	17	from	effects	761:767	arg1	survival					777:784	cell survival	772:784	cell survival	772:784	Chondrocytes were encapsulated into these hydrogels to detect their effects on cell survival, proliferation, morphology and ECM secretion.
32470590	1	18	theme	chemical	172:179	arg1	crosslinking					181:192	chemical crosslinking	172:192	chemical crosslinking	172:192	It is necessary to use chemical crosslinking to regulate the mechanical properties, biodegradability and biocompatibility of hydrogels.
32470590	8	19	theme	ECM	1163:1165	arg1	secretion					1167:1175	ECM secretion	1163:1175	ECM secretion of cells encapsulated in hydrogels	1163:1210	ECM secretion of cells encapsulated in hydrogels increased with the elongation of culture duration, and cells encapsulated in hydrogels HA-sNHS/Col I (HSC) and HA-CHO/Col I (HCC) secreted more ECM than others.
32470590	2	20	theme	type	324:327	arg1	hydrogels					360:368	collagen type I (Col I)/hyaluronic acid (HA) hydrogels	315:368	collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different chemical crosslinking manners	315:432	In this study, three kinds of collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different chemical crosslinking manners were designed and fabricated, and the effects of chemical crosslinking manners on the physical properties and biocompatibility of hydrogels were investigated.
32470590	9	21	theme	inflammatory	1453:1464	arg1	reaction					1466:1473	similar and acceptable inflammatory reaction	1430:1473	similar and acceptable inflammatory reaction	1430:1473	In vivo studies demonstrated that these hydrogels showed similar and acceptable inflammatory reaction.
32470590	2	22	theme	crosslinking	413:424	arg1	manners					426:432	different chemical crosslinking manners	394:432	different chemical crosslinking manners	394:432	In this study, three kinds of collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different chemical crosslinking manners were designed and fabricated, and the effects of chemical crosslinking manners on the physical properties and biocompatibility of hydrogels were investigated.
32470590	2	23	theme	collagen	315:322	arg1	Col					332:334	Col	332:334	Col	332:334	In this study, three kinds of collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different chemical crosslinking manners were designed and fabricated, and the effects of chemical crosslinking manners on the physical properties and biocompatibility of hydrogels were investigated.
32470590	2	23	theme	collagen	315:322	arg1	type					324:327	collagen type I	315:329	collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different chemical crosslinking manners	315:432	In this study, three kinds of collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different chemical crosslinking manners were designed and fabricated, and the effects of chemical crosslinking manners on the physical properties and biocompatibility of hydrogels were investigated.
32470590	2	24	theme	HA	356:357	arg1	hydrogels					360:368	collagen type I (Col I)/hyaluronic acid (HA) hydrogels	315:368	collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different chemical crosslinking manners	315:432	In this study, three kinds of collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different chemical crosslinking manners were designed and fabricated, and the effects of chemical crosslinking manners on the physical properties and biocompatibility of hydrogels were investigated.
32470590	0	25	theme	chemical	15:22	arg1	manners					37:43	chemical crosslinking manners	15:43	chemical crosslinking manners	15:43	The effects of chemical crosslinking manners on the physical properties and biocompatibility of collagen type I/hyaluronic acid composite hydrogels.
32470590	0	26	theme	composite	128:136	arg1	hydrogels					138:146	collagen type I/hyaluronic acid composite hydrogels	96:146	collagen type I/hyaluronic acid composite hydrogels	96:146	The effects of chemical crosslinking manners on the physical properties and biocompatibility of collagen type I/hyaluronic acid composite hydrogels.
32470590	0	27	dep	properties	61:70	arg1	the					48:50	the	48:50	the	48:50	The effects of chemical crosslinking manners on the physical properties and biocompatibility of collagen type I/hyaluronic acid composite hydrogels.
32470590	8	28	theme	hydrogels	1289:1297	arg1	HSC					1314:1316	HSC	1314:1316	HSC	1314:1316	ECM secretion of cells encapsulated in hydrogels increased with the elongation of culture duration, and cells encapsulated in hydrogels HA-sNHS/Col I (HSC) and HA-CHO/Col I (HCC) secreted more ECM than others.
32470590	8	28	theme	hydrogels	1289:1297	arg1	I					1311:1311	hydrogels HA-sNHS/Col I	1289:1311	hydrogels HA-sNHS/Col I (HSC)	1289:1317	ECM secretion of cells encapsulated in hydrogels increased with the elongation of culture duration, and cells encapsulated in hydrogels HA-sNHS/Col I (HSC) and HA-CHO/Col I (HCC) secreted more ECM than others.
32470590	2	29	theme	different	394:402	arg1	manners					426:432	different chemical crosslinking manners	394:432	different chemical crosslinking manners	394:432	In this study, three kinds of collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different chemical crosslinking manners were designed and fabricated, and the effects of chemical crosslinking manners on the physical properties and biocompatibility of hydrogels were investigated.
32470590	0	30	theme	acid	123:126	arg1	hydrogels					138:146	collagen type I/hyaluronic acid composite hydrogels	96:146	collagen type I/hyaluronic acid composite hydrogels	96:146	The effects of chemical crosslinking manners on the physical properties and biocompatibility of collagen type I/hyaluronic acid composite hydrogels.
32470590	0	31	theme	manners	37:43	arg1	effects					4:10	The effects	0:10	The effects of chemical crosslinking manners on the physical properties and biocompatibility of collagen type I/hyaluronic acid composite hydrogels.	0:147	The effects of chemical crosslinking manners on the physical properties and biocompatibility of collagen type I/hyaluronic acid composite hydrogels.
32470590	2	32	theme	same	379:382	arg1	ratio					384:388	the same ratio	375:388	the same ratio	375:388	In this study, three kinds of collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different chemical crosslinking manners were designed and fabricated, and the effects of chemical crosslinking manners on the physical properties and biocompatibility of hydrogels were investigated.
32470590	1	33	theme	mechanical	210:219	arg1	biocompatibility					254:269	biocompatibility	254:269	biocompatibility	254:269	It is necessary to use chemical crosslinking to regulate the mechanical properties, biodegradability and biocompatibility of hydrogels.
32470590	1	33	theme	mechanical	210:219	arg1	biodegradability					233:248	biodegradability	233:248	biodegradability	233:248	It is necessary to use chemical crosslinking to regulate the mechanical properties, biodegradability and biocompatibility of hydrogels.
32470590	1	33	theme	mechanical	210:219	arg1	properties					221:230	the mechanical properties	206:230	the mechanical properties	206:230	It is necessary to use chemical crosslinking to regulate the mechanical properties, biodegradability and biocompatibility of hydrogels.
32470590	0	34	theme	crosslinking	24:35	arg1	manners					37:43	chemical crosslinking manners	15:43	chemical crosslinking manners	15:43	The effects of chemical crosslinking manners on the physical properties and biocompatibility of collagen type I/hyaluronic acid composite hydrogels.
32470590	0	35	theme	hydrogels	138:146	arg1	biocompatibility					76:91	biocompatibility	76:91	biocompatibility	76:91	The effects of chemical crosslinking manners on the physical properties and biocompatibility of collagen type I/hyaluronic acid composite hydrogels.
32470590	0	35	theme	hydrogels	138:146	arg1	properties					61:70	physical properties	52:70	physical properties	52:70	The effects of chemical crosslinking manners on the physical properties and biocompatibility of collagen type I/hyaluronic acid composite hydrogels.
32470590	2	36	from	effects	472:478	arg1	biocompatibility					544:559	biocompatibility	544:559	biocompatibility	544:559	In this study, three kinds of collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different chemical crosslinking manners were designed and fabricated, and the effects of chemical crosslinking manners on the physical properties and biocompatibility of hydrogels were investigated.
32470590	2	36	from	effects	472:478	arg1	properties					529:538	physical properties	520:538	physical properties	520:538	In this study, three kinds of collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different chemical crosslinking manners were designed and fabricated, and the effects of chemical crosslinking manners on the physical properties and biocompatibility of hydrogels were investigated.
32470590	2	37	theme	chemical	404:411	arg1	manners					426:432	different chemical crosslinking manners	394:432	different chemical crosslinking manners	394:432	In this study, three kinds of collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different chemical crosslinking manners were designed and fabricated, and the effects of chemical crosslinking manners on the physical properties and biocompatibility of hydrogels were investigated.
32470590	6	38	theme	physical	1050:1057	arg1	properties					1059:1068	their physical properties	1044:1068	their physical properties	1044:1068	The results showed that chemical crosslinking manners of hydrogels could affect their physical properties to some extent.
32470590	7	39	theme	oval	1151:1154	arg1	shape					1156:1160	a round or oval shape	1140:1160	a round or oval shape	1140:1160	Chondrocytes encapsulated into these hydrogels showed a round or oval shape.
32470590	2	40	theme	manners	505:511	arg1	effects					472:478	the effects	468:478	the effects of chemical crosslinking manners on the physical properties and biocompatibility of hydrogels	468:572	In this study, three kinds of collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different chemical crosslinking manners were designed and fabricated, and the effects of chemical crosslinking manners on the physical properties and biocompatibility of hydrogels were investigated.
32470590	2	41	theme	hydrogels	564:572	arg1	biocompatibility					544:559	biocompatibility	544:559	biocompatibility	544:559	In this study, three kinds of collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different chemical crosslinking manners were designed and fabricated, and the effects of chemical crosslinking manners on the physical properties and biocompatibility of hydrogels were investigated.
32470590	2	41	theme	hydrogels	564:572	arg1	properties					529:538	physical properties	520:538	physical properties	520:538	In this study, three kinds of collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different chemical crosslinking manners were designed and fabricated, and the effects of chemical crosslinking manners on the physical properties and biocompatibility of hydrogels were investigated.
32470590	8	42	theme	HA-CHO/Col	1323:1332	arg1	I					1334:1334	HA-CHO/Col I	1323:1334	HA-CHO/Col I (HCC)	1323:1340	ECM secretion of cells encapsulated in hydrogels increased with the elongation of culture duration, and cells encapsulated in hydrogels HA-sNHS/Col I (HSC) and HA-CHO/Col I (HCC) secreted more ECM than others.
32470590	8	42	theme	HA-CHO/Col	1323:1332	arg1	HCC					1337:1339	HCC	1337:1339	HCC	1337:1339	ECM secretion of cells encapsulated in hydrogels increased with the elongation of culture duration, and cells encapsulated in hydrogels HA-sNHS/Col I (HSC) and HA-CHO/Col I (HCC) secreted more ECM than others.
32470590	8	43	theme	culture	1245:1251	arg1	duration					1253:1260	culture duration	1245:1260	culture duration	1245:1260	ECM secretion of cells encapsulated in hydrogels increased with the elongation of culture duration, and cells encapsulated in hydrogels HA-sNHS/Col I (HSC) and HA-CHO/Col I (HCC) secreted more ECM than others.
32470590	3	44	theme	hydrogels	663:671	arg1	swelling					633:640	swelling	633:640	swelling	633:640	The gelation time, mechanical property, swelling and degradability of hydrogels were characterized.
32470590	3	44	theme	hydrogels	663:671	arg1	time					606:609	The gelation time	593:609	The gelation time	593:609	The gelation time, mechanical property, swelling and degradability of hydrogels were characterized.
32470590	3	44	theme	hydrogels	663:671	arg1	property					623:630	mechanical property	612:630	mechanical property	612:630	The gelation time, mechanical property, swelling and degradability of hydrogels were characterized.
32470590	3	44	theme	hydrogels	663:671	arg1	degradability					646:658	degradability	646:658	degradability	646:658	The gelation time, mechanical property, swelling and degradability of hydrogels were characterized.
32470590	9	45	theme	In	1373:1374	arg1	studies					1381:1387	In vivo studies	1373:1387	In vivo studies	1373:1387	In vivo studies demonstrated that these hydrogels showed similar and acceptable inflammatory reaction.
32470590	4	46	theme	ECM	817:819	arg1	secretion					821:829	ECM secretion	817:829	ECM secretion	817:829	Chondrocytes were encapsulated into these hydrogels to detect their effects on cell survival, proliferation, morphology and ECM secretion.
32470590	8	47	theme	cells	1180:1184	arg1	secretion					1167:1175	ECM secretion	1163:1175	ECM secretion of cells encapsulated in hydrogels	1163:1210	ECM secretion of cells encapsulated in hydrogels increased with the elongation of culture duration, and cells encapsulated in hydrogels HA-sNHS/Col I (HSC) and HA-CHO/Col I (HCC) secreted more ECM than others.
32470590	3	48	theme	mechanical	612:621	arg1	property					623:630	mechanical property	612:630	mechanical property	612:630	The gelation time, mechanical property, swelling and degradability of hydrogels were characterized.
32470590	5	49	theme	SD	891:892	arg1	rats					894:897	SD rats	891:897	SD rats	891:897	Furthermore, the hydrogels were implanted into the back of SD rats to evaluate their biodegradability and biocompatibility in vivo.
32470590	8	50	theme	duration	1253:1260	arg1	elongation					1231:1240	the elongation	1227:1240	the elongation of culture duration	1227:1260	ECM secretion of cells encapsulated in hydrogels increased with the elongation of culture duration, and cells encapsulated in hydrogels HA-sNHS/Col I (HSC) and HA-CHO/Col I (HCC) secreted more ECM than others.
32470590	2	51	theme	chemical	483:490	arg1	manners					505:511	chemical crosslinking manners	483:511	chemical crosslinking manners	483:511	In this study, three kinds of collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different chemical crosslinking manners were designed and fabricated, and the effects of chemical crosslinking manners on the physical properties and biocompatibility of hydrogels were investigated.
32470590	8	52	theme	HA-sNHS/Col	1299:1309	arg1	HSC					1314:1316	HSC	1314:1316	HSC	1314:1316	ECM secretion of cells encapsulated in hydrogels increased with the elongation of culture duration, and cells encapsulated in hydrogels HA-sNHS/Col I (HSC) and HA-CHO/Col I (HCC) secreted more ECM than others.
32470590	8	52	theme	HA-sNHS/Col	1299:1309	arg1	I					1311:1311	hydrogels HA-sNHS/Col I	1289:1311	hydrogels HA-sNHS/Col I (HSC)	1289:1317	ECM secretion of cells encapsulated in hydrogels increased with the elongation of culture duration, and cells encapsulated in hydrogels HA-sNHS/Col I (HSC) and HA-CHO/Col I (HCC) secreted more ECM than others.
32470590	3	53	theme	gelation	597:604	arg1	time					606:609	The gelation time	593:609	The gelation time	593:609	The gelation time, mechanical property, swelling and degradability of hydrogels were characterized.
32470590	2	54	theme	hydrogels	360:368	arg1	kinds					306:310	three kinds	300:310	three kinds of collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different chemical crosslinking manners	300:432	In this study, three kinds of collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different chemical crosslinking manners were designed and fabricated, and the effects of chemical crosslinking manners on the physical properties and biocompatibility of hydrogels were investigated.
32470590	0	55	theme	physical	52:59	arg1	properties					61:70	physical properties	52:70	physical properties	52:70	The effects of chemical crosslinking manners on the physical properties and biocompatibility of collagen type I/hyaluronic acid composite hydrogels.
32470590	2	56	theme	acid	350:353	arg1	hydrogels					360:368	collagen type I (Col I)/hyaluronic acid (HA) hydrogels	315:368	collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different chemical crosslinking manners	315:432	In this study, three kinds of collagen type I (Col I)/hyaluronic acid (HA) hydrogels with the same ratio and different chemical crosslinking manners were designed and fabricated, and the effects of chemical crosslinking manners on the physical properties and biocompatibility of hydrogels were investigated.
32470590	6	57	theme	hydrogels	1021:1029	arg1	manners					1010:1016	chemical crosslinking manners	988:1016	chemical crosslinking manners of hydrogels	988:1029	The results showed that chemical crosslinking manners of hydrogels could affect their physical properties to some extent.
32569689	4	0	theme	carboxymethylcellulose	954:975	arg1	chains					977:982	the carboxymethylcellulose chains	950:982	the carboxymethylcellulose chains	950:982	The water absorption capacity of the hydrogels was studied as a function of temperature and pH. The results of FE-SEM showed the formation of ZnO particles in the hydrogel, while the water absorption capacity results supported the formation of zinc ion cross-linking within the carboxymethylcellulose chains.
32569689	4	1	theme	particles	822:830	arg1	formation					805:813	the formation	801:813	the formation of ZnO particles	801:830	The water absorption capacity of the hydrogels was studied as a function of temperature and pH. The results of FE-SEM showed the formation of ZnO particles in the hydrogel, while the water absorption capacity results supported the formation of zinc ion cross-linking within the carboxymethylcellulose chains.
32569689	4	1	theme	particles	822:830	arg1	capacity					697:704	The water absorption capacity	676:704	The water absorption capacity of the hydrogels	676:721	The water absorption capacity of the hydrogels was studied as a function of temperature and pH. The results of FE-SEM showed the formation of ZnO particles in the hydrogel, while the water absorption capacity results supported the formation of zinc ion cross-linking within the carboxymethylcellulose chains.
32569689	4	1	theme	particles	822:830	arg1	function					740:747	a function	738:747	a function of temperature	738:762	The water absorption capacity of the hydrogels was studied as a function of temperature and pH. The results of FE-SEM showed the formation of ZnO particles in the hydrogel, while the water absorption capacity results supported the formation of zinc ion cross-linking within the carboxymethylcellulose chains.
32569689	1	2	theme	functional	156:165	arg1	hydrogel					197:204	functional carboxymethyl cellulose (CMC) hydrogel	156:204	functional carboxymethyl cellulose (CMC) hydrogel crosslinked by divalent zinc ions	156:238	A green method was used for the preparation of functional carboxymethyl cellulose (CMC) hydrogel crosslinked by divalent zinc ions and incorporated with zinc oxide nanoparticles (ZnO) without using alkaline reagents.
32569689	0	3	theme	Zn2+	98:101	arg1	ions					103:106	Zn2+ ions	98:106	Zn2+ ions	98:106	Green and facile synthesis of carboxymethylcellulose/ZnO nanocomposite hydrogels crosslinked with Zn2+ ions.
32569689	4	4	theme	ZnO	818:820	arg1	particles					822:830	ZnO particles	818:830	ZnO particles	818:830	The water absorption capacity of the hydrogels was studied as a function of temperature and pH. The results of FE-SEM showed the formation of ZnO particles in the hydrogel, while the water absorption capacity results supported the formation of zinc ion cross-linking within the carboxymethylcellulose chains.
32569689	2	5	theme	sodium	342:347	arg1	matrix					405:410	a hydrogel matrix	394:410	a hydrogel matrix	394:410	In the process, sodium carboxymethyl cellulose was used not only as a hydrogel matrix but also for the release of free hydroxyl groups, which increases the pH of the reaction mixture, consequently leading to the formation of ZnO.
32569689	2	5	theme	sodium	342:347	arg1	cellulose					363:371	sodium carboxymethyl cellulose	342:371	sodium carboxymethyl cellulose	342:371	In the process, sodium carboxymethyl cellulose was used not only as a hydrogel matrix but also for the release of free hydroxyl groups, which increases the pH of the reaction mixture, consequently leading to the formation of ZnO.
32569689	1	6	theme	zinc	262:265	arg1	oxide					267:271	zinc oxide	262:271	zinc oxide nanoparticles (ZnO)	262:291	A green method was used for the preparation of functional carboxymethyl cellulose (CMC) hydrogel crosslinked by divalent zinc ions and incorporated with zinc oxide nanoparticles (ZnO) without using alkaline reagents.
32569689	2	7	theme	reaction	492:499	arg1	mixture					501:507	the reaction mixture	488:507	the reaction mixture	488:507	In the process, sodium carboxymethyl cellulose was used not only as a hydrogel matrix but also for the release of free hydroxyl groups, which increases the pH of the reaction mixture, consequently leading to the formation of ZnO.
32569689	5	8	theme	nanocomposite	997:1009	arg1	hydrogel					1011:1018	The CMC/ZnO nanocomposite hydrogel	985:1018	The CMC/ZnO nanocomposite hydrogel	985:1018	The CMC/ZnO nanocomposite hydrogel exhibited strong antimicrobial activity against pathogenic bacteria E. coli and L. monocytogenes.
32569689	4	9	theme	hydrogels	713:721	arg1	formation					805:813	the formation	801:813	the formation of ZnO particles	801:830	The water absorption capacity of the hydrogels was studied as a function of temperature and pH. The results of FE-SEM showed the formation of ZnO particles in the hydrogel, while the water absorption capacity results supported the formation of zinc ion cross-linking within the carboxymethylcellulose chains.
32569689	4	9	theme	hydrogels	713:721	arg1	function					740:747	a function	738:747	a function of temperature	738:762	The water absorption capacity of the hydrogels was studied as a function of temperature and pH. The results of FE-SEM showed the formation of ZnO particles in the hydrogel, while the water absorption capacity results supported the formation of zinc ion cross-linking within the carboxymethylcellulose chains.
32569689	4	9	theme	hydrogels	713:721	arg1	capacity					697:704	The water absorption capacity	676:704	The water absorption capacity of the hydrogels	676:721	The water absorption capacity of the hydrogels was studied as a function of temperature and pH. The results of FE-SEM showed the formation of ZnO particles in the hydrogel, while the water absorption capacity results supported the formation of zinc ion cross-linking within the carboxymethylcellulose chains.
32569689	1	10	theme	oxide	267:271	arg1	ZnO					288:290	ZnO	288:290	ZnO	288:290	A green method was used for the preparation of functional carboxymethyl cellulose (CMC) hydrogel crosslinked by divalent zinc ions and incorporated with zinc oxide nanoparticles (ZnO) without using alkaline reagents.
32569689	1	10	theme	oxide	267:271	arg1	nanoparticles					273:285	zinc oxide nanoparticles	262:285	zinc oxide nanoparticles (ZnO)	262:291	A green method was used for the preparation of functional carboxymethyl cellulose (CMC) hydrogel crosslinked by divalent zinc ions and incorporated with zinc oxide nanoparticles (ZnO) without using alkaline reagents.
32569689	2	11	theme	ZnO	551:553	arg1	formation					538:546	the formation	534:546	the formation of ZnO	534:553	In the process, sodium carboxymethyl cellulose was used not only as a hydrogel matrix but also for the release of free hydroxyl groups, which increases the pH of the reaction mixture, consequently leading to the formation of ZnO.
32569689	2	12	theme	carboxymethyl	349:361	arg1	matrix					405:410	a hydrogel matrix	394:410	a hydrogel matrix	394:410	In the process, sodium carboxymethyl cellulose was used not only as a hydrogel matrix but also for the release of free hydroxyl groups, which increases the pH of the reaction mixture, consequently leading to the formation of ZnO.
32569689	2	12	theme	carboxymethyl	349:361	arg1	cellulose					363:371	sodium carboxymethyl cellulose	342:371	sodium carboxymethyl cellulose	342:371	In the process, sodium carboxymethyl cellulose was used not only as a hydrogel matrix but also for the release of free hydroxyl groups, which increases the pH of the reaction mixture, consequently leading to the formation of ZnO.
32569689	1	13	theme	cellulose	181:189	arg1	hydrogel					197:204	functional carboxymethyl cellulose (CMC) hydrogel	156:204	functional carboxymethyl cellulose (CMC) hydrogel crosslinked by divalent zinc ions	156:238	A green method was used for the preparation of functional carboxymethyl cellulose (CMC) hydrogel crosslinked by divalent zinc ions and incorporated with zinc oxide nanoparticles (ZnO) without using alkaline reagents.
32569689	5	14	theme	L.	1100:1101	arg1	bacteria					1079:1086	pathogenic bacteria	1068:1086	pathogenic bacteria E. coli and L. monocytogenes	1068:1115	The CMC/ZnO nanocomposite hydrogel exhibited strong antimicrobial activity against pathogenic bacteria E. coli and L. monocytogenes.
32569689	0	15	theme	carboxymethylcellulose/ZnO	30:55	arg1	Green					0:4	Green	0:4	Green	0:4	Green and facile synthesis of carboxymethylcellulose/ZnO nanocomposite hydrogels crosslinked with Zn2+ ions.
32569689	0	15	theme	carboxymethylcellulose/ZnO	30:55	arg1	synthesis					17:25	facile synthesis	10:25	facile synthesis of carboxymethylcellulose/ZnO	10:55	Green and facile synthesis of carboxymethylcellulose/ZnO nanocomposite hydrogels crosslinked with Zn2+ ions.
32569689	1	16	theme	CMC	192:194	arg1	hydrogel					197:204	functional carboxymethyl cellulose (CMC) hydrogel	156:204	functional carboxymethyl cellulose (CMC) hydrogel crosslinked by divalent zinc ions	156:238	A green method was used for the preparation of functional carboxymethyl cellulose (CMC) hydrogel crosslinked by divalent zinc ions and incorporated with zinc oxide nanoparticles (ZnO) without using alkaline reagents.
32569689	5	17	theme	E.	1088:1089	arg1	bacteria					1079:1086	pathogenic bacteria	1068:1086	pathogenic bacteria E. coli and L. monocytogenes	1068:1115	The CMC/ZnO nanocomposite hydrogel exhibited strong antimicrobial activity against pathogenic bacteria E. coli and L. monocytogenes.
32569689	5	18	theme	pathogenic	1068:1077	arg1	bacteria					1079:1086	pathogenic bacteria	1068:1086	pathogenic bacteria E. coli and L. monocytogenes	1068:1115	The CMC/ZnO nanocomposite hydrogel exhibited strong antimicrobial activity against pathogenic bacteria E. coli and L. monocytogenes.
32569689	4	19	theme	absorption	865:874	arg1	capacity					876:883	the water absorption capacity	855:883	the water absorption capacity	855:883	The water absorption capacity of the hydrogels was studied as a function of temperature and pH. The results of FE-SEM showed the formation of ZnO particles in the hydrogel, while the water absorption capacity results supported the formation of zinc ion cross-linking within the carboxymethylcellulose chains.
32569689	1	20	theme	green	111:115	arg1	method					117:122	A green method	109:122	A green method	109:122	A green method was used for the preparation of functional carboxymethyl cellulose (CMC) hydrogel crosslinked by divalent zinc ions and incorporated with zinc oxide nanoparticles (ZnO) without using alkaline reagents.
32569689	1	21	theme	hydrogel	197:204	arg1	preparation					141:151	the preparation	137:151	the preparation of functional carboxymethyl cellulose (CMC) hydrogel crosslinked by divalent zinc ions	137:238	A green method was used for the preparation of functional carboxymethyl cellulose (CMC) hydrogel crosslinked by divalent zinc ions and incorporated with zinc oxide nanoparticles (ZnO) without using alkaline reagents.
32569689	0	22	theme	facile	10:15	arg1	synthesis					17:25	facile synthesis	10:25	facile synthesis of carboxymethylcellulose/ZnO	10:55	Green and facile synthesis of carboxymethylcellulose/ZnO nanocomposite hydrogels crosslinked with Zn2+ ions.
32569689	4	23	theme	water	859:863	arg1	capacity					876:883	the water absorption capacity	855:883	the water absorption capacity	855:883	The water absorption capacity of the hydrogels was studied as a function of temperature and pH. The results of FE-SEM showed the formation of ZnO particles in the hydrogel, while the water absorption capacity results supported the formation of zinc ion cross-linking within the carboxymethylcellulose chains.
32569689	1	24	theme	carboxymethyl	167:179	arg1	hydrogel					197:204	functional carboxymethyl cellulose (CMC) hydrogel	156:204	functional carboxymethyl cellulose (CMC) hydrogel crosslinked by divalent zinc ions	156:238	A green method was used for the preparation of functional carboxymethyl cellulose (CMC) hydrogel crosslinked by divalent zinc ions and incorporated with zinc oxide nanoparticles (ZnO) without using alkaline reagents.
32569689	2	25	theme	groups	454:459	arg1	release					429:435	the release	425:435	the release	425:435	In the process, sodium carboxymethyl cellulose was used not only as a hydrogel matrix but also for the release of free hydroxyl groups, which increases the pH of the reaction mixture, consequently leading to the formation of ZnO.
32569689	4	26	dep	results	776:782	arg1	pH.					768:770	pH. The results of FE-SEM	768:792	pH. The results of FE-SEM	768:792	The water absorption capacity of the hydrogels was studied as a function of temperature and pH. The results of FE-SEM showed the formation of ZnO particles in the hydrogel, while the water absorption capacity results supported the formation of zinc ion cross-linking within the carboxymethylcellulose chains.
32569689	2	27	theme	hydrogel	396:403	arg1	cellulose					363:371	sodium carboxymethyl cellulose	342:371	sodium carboxymethyl cellulose	342:371	In the process, sodium carboxymethyl cellulose was used not only as a hydrogel matrix but also for the release of free hydroxyl groups, which increases the pH of the reaction mixture, consequently leading to the formation of ZnO.
32569689	2	27	theme	hydrogel	396:403	arg1	matrix					405:410	a hydrogel matrix	394:410	a hydrogel matrix	394:410	In the process, sodium carboxymethyl cellulose was used not only as a hydrogel matrix but also for the release of free hydroxyl groups, which increases the pH of the reaction mixture, consequently leading to the formation of ZnO.
32569689	2	28	theme	hydroxyl	445:452	arg1	groups					454:459	free hydroxyl groups	440:459	free hydroxyl groups	440:459	In the process, sodium carboxymethyl cellulose was used not only as a hydrogel matrix but also for the release of free hydroxyl groups, which increases the pH of the reaction mixture, consequently leading to the formation of ZnO.
32569689	5	29	dep	L.	1100:1101	arg1	monocytogenes					1103:1115	monocytogenes	1103:1115	monocytogenes	1103:1115	The CMC/ZnO nanocomposite hydrogel exhibited strong antimicrobial activity against pathogenic bacteria E. coli and L. monocytogenes.
32569689	2	30	theme	mixture	501:507	arg1	pH					482:483	the pH	478:483	the pH of the reaction mixture	478:507	In the process, sodium carboxymethyl cellulose was used not only as a hydrogel matrix but also for the release of free hydroxyl groups, which increases the pH of the reaction mixture, consequently leading to the formation of ZnO.
32569689	3	31	theme	chemical	560:567	arg1	structure					569:577	chemical structure	560:577	chemical structure	560:577	The chemical structure and morphology of the hydrogels were characterized using FTIR and FE-SEM analysis, respectively.
32569689	1	32	theme	divalent	221:228	arg1	ions					235:238	divalent zinc ions	221:238	divalent zinc ions	221:238	A green method was used for the preparation of functional carboxymethyl cellulose (CMC) hydrogel crosslinked by divalent zinc ions and incorporated with zinc oxide nanoparticles (ZnO) without using alkaline reagents.
32569689	2	33	theme	free	440:443	arg1	groups					454:459	free hydroxyl groups	440:459	free hydroxyl groups	440:459	In the process, sodium carboxymethyl cellulose was used not only as a hydrogel matrix but also for the release of free hydroxyl groups, which increases the pH of the reaction mixture, consequently leading to the formation of ZnO.
32569689	1	34	theme	alkaline	307:314	arg1	reagents					316:323	alkaline reagents	307:323	alkaline reagents	307:323	A green method was used for the preparation of functional carboxymethyl cellulose (CMC) hydrogel crosslinked by divalent zinc ions and incorporated with zinc oxide nanoparticles (ZnO) without using alkaline reagents.
32569689	3	35	theme	hydrogels	601:609	arg1	morphology					583:592	morphology	583:592	morphology	583:592	The chemical structure and morphology of the hydrogels were characterized using FTIR and FE-SEM analysis, respectively.
32569689	3	35	theme	hydrogels	601:609	arg1	structure					569:577	chemical structure	560:577	chemical structure	560:577	The chemical structure and morphology of the hydrogels were characterized using FTIR and FE-SEM analysis, respectively.
32569689	4	36	theme	FE-SEM	787:792	arg1	results					776:782	The results	772:782	pH. The results of FE-SEM	768:792	The water absorption capacity of the hydrogels was studied as a function of temperature and pH. The results of FE-SEM showed the formation of ZnO particles in the hydrogel, while the water absorption capacity results supported the formation of zinc ion cross-linking within the carboxymethylcellulose chains.
32569689	4	37	theme	water	680:684	arg1	formation					805:813	the formation	801:813	the formation of ZnO particles	801:830	The water absorption capacity of the hydrogels was studied as a function of temperature and pH. The results of FE-SEM showed the formation of ZnO particles in the hydrogel, while the water absorption capacity results supported the formation of zinc ion cross-linking within the carboxymethylcellulose chains.
32569689	4	37	theme	water	680:684	arg1	function					740:747	a function	738:747	a function of temperature	738:762	The water absorption capacity of the hydrogels was studied as a function of temperature and pH. The results of FE-SEM showed the formation of ZnO particles in the hydrogel, while the water absorption capacity results supported the formation of zinc ion cross-linking within the carboxymethylcellulose chains.
32569689	4	37	theme	water	680:684	arg1	capacity					697:704	The water absorption capacity	676:704	The water absorption capacity of the hydrogels	676:721	The water absorption capacity of the hydrogels was studied as a function of temperature and pH. The results of FE-SEM showed the formation of ZnO particles in the hydrogel, while the water absorption capacity results supported the formation of zinc ion cross-linking within the carboxymethylcellulose chains.
32569689	5	38	theme	strong	1030:1035	arg1	activity					1051:1058	strong antimicrobial activity	1030:1058	strong antimicrobial activity against pathogenic bacteria E. coli and L. monocytogenes	1030:1115	The CMC/ZnO nanocomposite hydrogel exhibited strong antimicrobial activity against pathogenic bacteria E. coli and L. monocytogenes.
32569689	3	39	theme	FE-SEM	645:650	arg1	analysis					652:659	FE-SEM analysis	645:659	FE-SEM analysis	645:659	The chemical structure and morphology of the hydrogels were characterized using FTIR and FE-SEM analysis, respectively.
32569689	4	40	theme	temperature	752:762	arg1	formation					805:813	the formation	801:813	the formation of ZnO particles	801:830	The water absorption capacity of the hydrogels was studied as a function of temperature and pH. The results of FE-SEM showed the formation of ZnO particles in the hydrogel, while the water absorption capacity results supported the formation of zinc ion cross-linking within the carboxymethylcellulose chains.
32569689	4	40	theme	temperature	752:762	arg1	capacity					697:704	The water absorption capacity	676:704	The water absorption capacity of the hydrogels	676:721	The water absorption capacity of the hydrogels was studied as a function of temperature and pH. The results of FE-SEM showed the formation of ZnO particles in the hydrogel, while the water absorption capacity results supported the formation of zinc ion cross-linking within the carboxymethylcellulose chains.
32569689	4	40	theme	temperature	752:762	arg1	function					740:747	a function	738:747	a function of temperature	738:762	The water absorption capacity of the hydrogels was studied as a function of temperature and pH. The results of FE-SEM showed the formation of ZnO particles in the hydrogel, while the water absorption capacity results supported the formation of zinc ion cross-linking within the carboxymethylcellulose chains.
32569689	5	41	theme	CMC/ZnO	989:995	arg1	hydrogel					1011:1018	The CMC/ZnO nanocomposite hydrogel	985:1018	The CMC/ZnO nanocomposite hydrogel	985:1018	The CMC/ZnO nanocomposite hydrogel exhibited strong antimicrobial activity against pathogenic bacteria E. coli and L. monocytogenes.
32569689	1	42	used	used	128:131	arg2	method					117:122	A green method	109:122	A green method	109:122	A green method was used for the preparation of functional carboxymethyl cellulose (CMC) hydrogel crosslinked by divalent zinc ions and incorporated with zinc oxide nanoparticles (ZnO) without using alkaline reagents.
32569689	1	43	theme	zinc	230:233	arg1	ions					235:238	divalent zinc ions	221:238	divalent zinc ions	221:238	A green method was used for the preparation of functional carboxymethyl cellulose (CMC) hydrogel crosslinked by divalent zinc ions and incorporated with zinc oxide nanoparticles (ZnO) without using alkaline reagents.
32569689	5	44	theme	antimicrobial	1037:1049	arg1	activity					1051:1058	strong antimicrobial activity	1030:1058	strong antimicrobial activity against pathogenic bacteria E. coli and L. monocytogenes	1030:1115	The CMC/ZnO nanocomposite hydrogel exhibited strong antimicrobial activity against pathogenic bacteria E. coli and L. monocytogenes.
32569689	2	45	used	used	377:380	arg2	cellulose					363:371	sodium carboxymethyl cellulose	342:371	sodium carboxymethyl cellulose	342:371	In the process, sodium carboxymethyl cellulose was used not only as a hydrogel matrix but also for the release of free hydroxyl groups, which increases the pH of the reaction mixture, consequently leading to the formation of ZnO.
32569689	2	45	used	used	377:380	arg2	matrix					405:410	a hydrogel matrix	394:410	a hydrogel matrix	394:410	In the process, sodium carboxymethyl cellulose was used not only as a hydrogel matrix but also for the release of free hydroxyl groups, which increases the pH of the reaction mixture, consequently leading to the formation of ZnO.
32569689	4	46	theme	absorption	686:695	arg1	formation					805:813	the formation	801:813	the formation of ZnO particles	801:830	The water absorption capacity of the hydrogels was studied as a function of temperature and pH. The results of FE-SEM showed the formation of ZnO particles in the hydrogel, while the water absorption capacity results supported the formation of zinc ion cross-linking within the carboxymethylcellulose chains.
32569689	4	46	theme	absorption	686:695	arg1	function					740:747	a function	738:747	a function of temperature	738:762	The water absorption capacity of the hydrogels was studied as a function of temperature and pH. The results of FE-SEM showed the formation of ZnO particles in the hydrogel, while the water absorption capacity results supported the formation of zinc ion cross-linking within the carboxymethylcellulose chains.
32569689	4	46	theme	absorption	686:695	arg1	capacity					697:704	The water absorption capacity	676:704	The water absorption capacity of the hydrogels	676:721	The water absorption capacity of the hydrogels was studied as a function of temperature and pH. The results of FE-SEM showed the formation of ZnO particles in the hydrogel, while the water absorption capacity results supported the formation of zinc ion cross-linking within the carboxymethylcellulose chains.
32569689	5	47	dep	E.	1088:1089	arg1	coli					1091:1094	coli	1091:1094	coli	1091:1094	The CMC/ZnO nanocomposite hydrogel exhibited strong antimicrobial activity against pathogenic bacteria E. coli and L. monocytogenes.
32569689	4	48	theme	ion	925:927	arg1	formation					907:915	the formation	903:915	the formation of zinc ion cross-linking within the carboxymethylcellulose chains	903:982	The water absorption capacity of the hydrogels was studied as a function of temperature and pH. The results of FE-SEM showed the formation of ZnO particles in the hydrogel, while the water absorption capacity results supported the formation of zinc ion cross-linking within the carboxymethylcellulose chains.
32569689	3	49	dep	structure	569:577	arg1	The					556:558	The	556:558	The	556:558	The chemical structure and morphology of the hydrogels were characterized using FTIR and FE-SEM analysis, respectively.
32569689	4	50	dep	results	885:891	arg1	supported					893:901	supported	893:901	results supported the formation of zinc ion cross-linking within the carboxymethylcellulose chains	885:982	The water absorption capacity of the hydrogels was studied as a function of temperature and pH. The results of FE-SEM showed the formation of ZnO particles in the hydrogel, while the water absorption capacity results supported the formation of zinc ion cross-linking within the carboxymethylcellulose chains.
32569689	4	51	theme	zinc	920:923	arg1	ion					925:927	zinc ion	920:927	zinc ion cross-linking within the carboxymethylcellulose chains	920:982	The water absorption capacity of the hydrogels was studied as a function of temperature and pH. The results of FE-SEM showed the formation of ZnO particles in the hydrogel, while the water absorption capacity results supported the formation of zinc ion cross-linking within the carboxymethylcellulose chains.
34416386	5	0	theme	RNA	856:858	arg1	interference					860:871	RNA interference	856:871	RNA interference	856:871	The functions of identified ECM components were interrogated using RNA interference.
34416386	2	1	theme	functions	390:398	arg1	dissection					356:365	the systematic dissection	341:365	the systematic dissection of the composition and functions of the ECM	341:409	However, only a few research organisms are available for the systematic dissection of the composition and functions of the ECM, particularly during regeneration.
34416386	1	2	theme	macromolecules	183:196	arg1	network					172:178	a three-dimensional network	152:178	a three-dimensional network of macromolecules that provides a microenvironment capable of supporting and regulating cell functions	152:281	The extracellular matrix (ECM) is a three-dimensional network of macromolecules that provides a microenvironment capable of supporting and regulating cell functions.
34416386	1	2	theme	macromolecules	183:196	arg1	matrix					136:141	The extracellular matrix	118:141	The extracellular matrix (ECM)	118:147	The extracellular matrix (ECM) is a three-dimensional network of macromolecules that provides a microenvironment capable of supporting and regulating cell functions.
34416386	5	3	theme	identified	806:815	arg1	components					821:830	identified ECM components	806:830	identified ECM components	806:830	The functions of identified ECM components were interrogated using RNA interference.
34416386	6	4	theme	tissue	955:960	arg1	regeneration					962:973	tissue regeneration	955:973	tissue regeneration	955:973	Using this approach, we found that heparan sulfate proteoglycan is essential for tissue regeneration.
34416386	2	5	theme	systematic	345:354	arg1	dissection					356:365	the systematic dissection	341:365	the systematic dissection of the composition and functions of the ECM	341:409	However, only a few research organisms are available for the systematic dissection of the composition and functions of the ECM, particularly during regeneration.
34416386	0	6	from	Matrix	84:89	arg1	Regeneration					104:115	Planarian Regeneration	94:115	Planarian Regeneration	94:115	Decellularization Enables Characterization and Functional Analysis of Extracellular Matrix in Planarian Regeneration.
34416386	7	7	theme	novel	1054:1058	arg1	components					1064:1073	both known and novel ECM components	1039:1073	both known and novel ECM components involved in regeneration	1039:1098	Our strategy provides an experimental approach for identifying both known and novel ECM components involved in regeneration.
34416386	5	8	theme	components	821:830	arg1	functions					793:801	The functions	789:801	The functions of identified ECM components	789:830	The functions of identified ECM components were interrogated using RNA interference.
34416386	7	9	theme	ECM	1060:1062	arg1	components					1064:1073	both known and novel ECM components	1039:1073	both known and novel ECM components involved in regeneration	1039:1098	Our strategy provides an experimental approach for identifying both known and novel ECM components involved in regeneration.
34416386	2	10	theme	research	304:311	arg1	organisms					313:321	only a few research organisms	293:321	only a few research organisms	293:321	However, only a few research organisms are available for the systematic dissection of the composition and functions of the ECM, particularly during regeneration.
34416386	3	11	theme	flatworm	474:481	arg1	mediterranea					493:504	the free-living flatworm Schmidtea mediterranea	458:504	the free-living flatworm Schmidtea mediterranea	458:504	We utilized the free-living flatworm Schmidtea mediterranea to develop an integrative approach consisting of decellularization, proteomics, and RNAi to characterize and investigate ECM functions during tissue homeostasis and regeneration.
34416386	7	12	theme	known	1044:1048	arg1	components					1064:1073	both known and novel ECM components	1039:1073	both known and novel ECM components involved in regeneration	1039:1098	Our strategy provides an experimental approach for identifying both known and novel ECM components involved in regeneration.
34416386	3	13	theme	Schmidtea	483:491	arg1	mediterranea					493:504	the free-living flatworm Schmidtea mediterranea	458:504	the free-living flatworm Schmidtea mediterranea	458:504	We utilized the free-living flatworm Schmidtea mediterranea to develop an integrative approach consisting of decellularization, proteomics, and RNAi to characterize and investigate ECM functions during tissue homeostasis and regeneration.
34416386	0	14	from	Analysis	58:65	arg1	Regeneration					104:115	Planarian Regeneration	94:115	Planarian Regeneration	94:115	Decellularization Enables Characterization and Functional Analysis of Extracellular Matrix in Planarian Regeneration.
34416386	3	15	theme	ECM	627:629	arg1	functions					631:639	ECM functions	627:639	ECM functions	627:639	We utilized the free-living flatworm Schmidtea mediterranea to develop an integrative approach consisting of decellularization, proteomics, and RNAi to characterize and investigate ECM functions during tissue homeostasis and regeneration.
34416386	1	16	theme	extracellular	122:134	arg1	network					172:178	a three-dimensional network	152:178	a three-dimensional network of macromolecules that provides a microenvironment capable of supporting and regulating cell functions	152:281	The extracellular matrix (ECM) is a three-dimensional network of macromolecules that provides a microenvironment capable of supporting and regulating cell functions.
34416386	1	16	theme	extracellular	122:134	arg1	ECM					144:146	ECM	144:146	ECM	144:146	The extracellular matrix (ECM) is a three-dimensional network of macromolecules that provides a microenvironment capable of supporting and regulating cell functions.
34416386	1	16	theme	extracellular	122:134	arg1	matrix					136:141	The extracellular matrix	118:141	The extracellular matrix (ECM)	118:147	The extracellular matrix (ECM) is a three-dimensional network of macromolecules that provides a microenvironment capable of supporting and regulating cell functions.
34416386	2	17	theme	ECM	407:409	arg1	functions					390:398	functions	390:398	functions	390:398	However, only a few research organisms are available for the systematic dissection of the composition and functions of the ECM, particularly during regeneration.
34416386	2	17	theme	ECM	407:409	arg1	composition					374:384	composition	374:384	composition	374:384	However, only a few research organisms are available for the systematic dissection of the composition and functions of the ECM, particularly during regeneration.
34416386	0	18	theme	Functional	47:56	arg1	Analysis					58:65	Characterization and Functional Analysis	26:65	Characterization and Functional Analysis of Extracellular Matrix in Planarian Regeneration	26:115	Decellularization Enables Characterization and Functional Analysis of Extracellular Matrix in Planarian Regeneration.
34416386	2	19	dep	composition	374:384	arg1	the					370:372	the	370:372	the	370:372	However, only a few research organisms are available for the systematic dissection of the composition and functions of the ECM, particularly during regeneration.
34416386	1	20	theme	capable	231:237	arg1	microenvironment					214:229	a microenvironment	212:229	a microenvironment capable of supporting and regulating cell functions	212:281	The extracellular matrix (ECM) is a three-dimensional network of macromolecules that provides a microenvironment capable of supporting and regulating cell functions.
34416386	0	21	theme	Characterization and	26:45	arg1	Analysis					58:65	Characterization and Functional Analysis	26:65	Characterization and Functional Analysis of Extracellular Matrix in Planarian Regeneration	26:115	Decellularization Enables Characterization and Functional Analysis of Extracellular Matrix in Planarian Regeneration.
34416386	0	22	from	Regeneration	104:115	arg1	Analysis					58:65	Characterization and Functional Analysis	26:65	Characterization and Functional Analysis of Extracellular Matrix in Planarian Regeneration	26:115	Decellularization Enables Characterization and Functional Analysis of Extracellular Matrix in Planarian Regeneration.
34416386	3	23	theme	free-living	462:472	arg1	mediterranea					493:504	the free-living flatworm Schmidtea mediterranea	458:504	the free-living flatworm Schmidtea mediterranea	458:504	We utilized the free-living flatworm Schmidtea mediterranea to develop an integrative approach consisting of decellularization, proteomics, and RNAi to characterize and investigate ECM functions during tissue homeostasis and regeneration.
34416386	5	24	theme	ECM	817:819	arg1	components					821:830	identified ECM components	806:830	identified ECM components	806:830	The functions of identified ECM components were interrogated using RNA interference.
34416386	0	25	theme	Matrix	84:89	arg1	Analysis					58:65	Characterization and Functional Analysis	26:65	Characterization and Functional Analysis of Extracellular Matrix in Planarian Regeneration	26:115	Decellularization Enables Characterization and Functional Analysis of Extracellular Matrix in Planarian Regeneration.
34416386	2	26	theme	composition	374:384	arg1	dissection					356:365	the systematic dissection	341:365	the systematic dissection of the composition and functions of the ECM	341:409	However, only a few research organisms are available for the systematic dissection of the composition and functions of the ECM, particularly during regeneration.
34416386	0	27	theme	Extracellular	70:82	arg1	Matrix					84:89	Extracellular Matrix	70:89	Extracellular Matrix in Planarian Regeneration	70:115	Decellularization Enables Characterization and Functional Analysis of Extracellular Matrix in Planarian Regeneration.
34416386	3	28	theme	tissue	648:653	arg1	homeostasis					655:665	tissue homeostasis	648:665	tissue homeostasis	648:665	We utilized the free-living flatworm Schmidtea mediterranea to develop an integrative approach consisting of decellularization, proteomics, and RNAi to characterize and investigate ECM functions during tissue homeostasis and regeneration.
34416386	3	29	used	utilized	449:456	arg2	We					446:447	We	446:447	We	446:447	We utilized the free-living flatworm Schmidtea mediterranea to develop an integrative approach consisting of decellularization, proteomics, and RNAi to characterize and investigate ECM functions during tissue homeostasis and regeneration.
34416386	4	30	attach	isolated	711:718	arg2	samples					698:704	ECM-enriched samples	685:704	ECM-enriched samples	685:704	ECM-enriched samples were isolated from planarians, and their proteomes were characterized by LC-MS/MS.
34416386	4	30	attach	isolated	711:718	arg1	planarians					725:734	planarians	725:734	planarians	725:734	ECM-enriched samples were isolated from planarians, and their proteomes were characterized by LC-MS/MS.
34416386	3	31	theme	integrative	520:530	arg1	approach					532:539	an integrative approach	517:539	an integrative approach consisting of decellularization, proteomics, and RNAi	517:593	We utilized the free-living flatworm Schmidtea mediterranea to develop an integrative approach consisting of decellularization, proteomics, and RNAi to characterize and investigate ECM functions during tissue homeostasis and regeneration.
34416386	6	32	theme	sulfate	917:923	arg1	proteoglycan					925:936	heparan sulfate proteoglycan	909:936	heparan sulfate proteoglycan	909:936	Using this approach, we found that heparan sulfate proteoglycan is essential for tissue regeneration.
34416386	1	33	theme	cell	268:271	arg1	functions					273:281	cell functions	268:281	cell functions	268:281	The extracellular matrix (ECM) is a three-dimensional network of macromolecules that provides a microenvironment capable of supporting and regulating cell functions.
34416386	0	34	theme	Planarian	94:102	arg1	Regeneration					104:115	Planarian Regeneration	94:115	Planarian Regeneration	94:115	Decellularization Enables Characterization and Functional Analysis of Extracellular Matrix in Planarian Regeneration.
34416386	6	35	theme	heparan	909:915	arg1	proteoglycan					925:936	heparan sulfate proteoglycan	909:936	heparan sulfate proteoglycan	909:936	Using this approach, we found that heparan sulfate proteoglycan is essential for tissue regeneration.
34416386	1	36	theme	three-dimensional	154:170	arg1	network					172:178	a three-dimensional network	152:178	a three-dimensional network of macromolecules that provides a microenvironment capable of supporting and regulating cell functions	152:281	The extracellular matrix (ECM) is a three-dimensional network of macromolecules that provides a microenvironment capable of supporting and regulating cell functions.
34416386	1	36	theme	three-dimensional	154:170	arg1	matrix					136:141	The extracellular matrix	118:141	The extracellular matrix (ECM)	118:147	The extracellular matrix (ECM) is a three-dimensional network of macromolecules that provides a microenvironment capable of supporting and regulating cell functions.
34416386	7	37	theme	experimental	1001:1012	arg1	approach					1014:1021	an experimental approach	998:1021	an experimental approach for identifying both known and novel ECM components involved in regeneration	998:1098	Our strategy provides an experimental approach for identifying both known and novel ECM components involved in regeneration.
34416386	4	38	theme	ECM-enriched	685:696	arg1	samples					698:704	ECM-enriched samples	685:704	ECM-enriched samples	685:704	ECM-enriched samples were isolated from planarians, and their proteomes were characterized by LC-MS/MS.
32448603	4	0	theme	cells	736:740	arg1	metabolism					712:721	the metabolism	708:721	the metabolism of C6 glioma cells and neural stem cells (NSCs)	708:769	Hydrogels based on low-esterified pectin significantly slow down the metabolism of C6 glioma cells and neural stem cells (NSCs) and slightly decrease the viability of the C6 glioma, but not of NSCs.
32448603	1	1	theme	common	159:164	arg1	Glioblastoma					134:145	Glioblastoma	134:145	Glioblastoma	134:145	Glioblastoma is the most common malignant tumor of the brain, but its treatment outcomes can be improved by new therapeutic techniques using biocompatible materials.
32448603	1	1	theme	common	159:164	arg1	tumor					176:180	the most common malignant tumor	150:180	the most common malignant tumor of the brain	150:193	Glioblastoma is the most common malignant tumor of the brain, but its treatment outcomes can be improved by new therapeutic techniques using biocompatible materials.
32448603	6	2	theme	pectin	983:988	arg1	hydrogels					990:998	pectin hydrogels	983:998	pectin hydrogels supplemented with different ratios of two ECM proteins-collagens I and IV	983:1072	We created a set of pectin hydrogels supplemented with different ratios of two ECM proteins-collagens I and IV.
32448603	6	3	theme	ECM	1042:1044	arg1	I					1065:1065	I	1065:1065	I	1065:1065	We created a set of pectin hydrogels supplemented with different ratios of two ECM proteins-collagens I and IV.
32448603	6	3	theme	ECM	1042:1044	arg1	proteins-collagens					1046:1063	two ECM proteins-collagens I and IV	1038:1072	two ECM proteins-collagens I and IV	1038:1072	We created a set of pectin hydrogels supplemented with different ratios of two ECM proteins-collagens I and IV.
32448603	6	3	theme	ECM	1042:1044	arg1	IV					1071:1072	IV	1071:1072	IV	1071:1072	We created a set of pectin hydrogels supplemented with different ratios of two ECM proteins-collagens I and IV.
32448603	5	4	theme	proliferation	881:893	arg1	rate					895:898	cell proliferation rate	876:898	cell proliferation rate	876:898	This happens due to a decrease in cell proliferation rate, while apoptosis degrees remain stable or negligibly decrease.
32448603	1	5	theme	malignant	166:174	arg1	Glioblastoma					134:145	Glioblastoma	134:145	Glioblastoma	134:145	Glioblastoma is the most common malignant tumor of the brain, but its treatment outcomes can be improved by new therapeutic techniques using biocompatible materials.
32448603	1	5	theme	malignant	166:174	arg1	tumor					176:180	the most common malignant tumor	150:180	the most common malignant tumor of the brain	150:193	Glioblastoma is the most common malignant tumor of the brain, but its treatment outcomes can be improved by new therapeutic techniques using biocompatible materials.
32448603	1	6	theme	new	242:244	arg1	techniques					258:267	new therapeutic techniques	242:267	new therapeutic techniques using biocompatible materials	242:297	Glioblastoma is the most common malignant tumor of the brain, but its treatment outcomes can be improved by new therapeutic techniques using biocompatible materials.
32448603	7	7	from	gels	1200:1203	arg1	ratio					1171:1175	the ratio	1167:1175	the ratio of two ECM proteins in gels used for 3D cell cultivation	1167:1232	We have shown that the formation of cell processes in glioma C6 can be regulated by varying the ratio of two ECM proteins in gels used for 3D cell cultivation.
32448603	5	8	theme	cell	876:879	arg1	rate					895:898	cell proliferation rate	876:898	cell proliferation rate	876:898	This happens due to a decrease in cell proliferation rate, while apoptosis degrees remain stable or negligibly decrease.
32448603	9	9	theme	cell	1518:1521	arg1	dynamics					1523:1530	glioma cell dynamics	1511:1530	glioma cell dynamics	1511:1530	The results presented suggest that modified pectins supplemented with two collagen types may serve as prospective biomaterials for glioblastoma treatment due to their ability to regulate glioma cell dynamics.
32448603	1	10	theme	therapeutic	246:256	arg1	techniques					258:267	new therapeutic techniques	242:267	new therapeutic techniques using biocompatible materials	242:297	Glioblastoma is the most common malignant tumor of the brain, but its treatment outcomes can be improved by new therapeutic techniques using biocompatible materials.
32448603	0	11	theme	prospective	82:92	arg1	behavior					70:77	neural cell behavior	58:77	neural cell behavior	58:77	Hydrogels based on modified pectins capable of modulating neural cell behavior as prospective biomaterials in glioblastoma treatment.
32448603	0	11	theme	prospective	82:92	arg1	biomaterials					94:105	prospective biomaterials	82:105	prospective biomaterials in glioblastoma treatment	82:131	Hydrogels based on modified pectins capable of modulating neural cell behavior as prospective biomaterials in glioblastoma treatment.
32448603	3	12	theme	gels	581:584	arg1	properties					563:572	visco-elastic properties	549:572	visco-elastic properties of the gels	549:584	We discovered optimal gelation conditions in the presence of Ca2+ by the analysis of visco-elastic properties of the gels and produced a series of biomaterials in hydrogel forms.
32448603	2	13	theme	27.4	386:389	arg1	%					390:390	%	390:390	%	390:390	Utilizing controllable alkaline de-esterification we obtained pectin preparation with 27.4% esterification degree and used it for bio-artificial matrix production.
32448603	4	14	theme	stem	753:756	arg1	NSCs					765:768	NSCs	765:768	NSCs	765:768	Hydrogels based on low-esterified pectin significantly slow down the metabolism of C6 glioma cells and neural stem cells (NSCs) and slightly decrease the viability of the C6 glioma, but not of NSCs.
32448603	4	14	theme	stem	753:756	arg1	cells					758:762	neural stem cells	746:762	neural stem cells (NSCs)	746:769	Hydrogels based on low-esterified pectin significantly slow down the metabolism of C6 glioma cells and neural stem cells (NSCs) and slightly decrease the viability of the C6 glioma, but not of NSCs.
32448603	0	15	theme	glioblastoma	110:121	arg1	treatment					123:131	glioblastoma treatment	110:131	glioblastoma treatment	110:131	Hydrogels based on modified pectins capable of modulating neural cell behavior as prospective biomaterials in glioblastoma treatment.
32448603	4	16	theme	neural	746:751	arg1	NSCs					765:768	NSCs	765:768	NSCs	765:768	Hydrogels based on low-esterified pectin significantly slow down the metabolism of C6 glioma cells and neural stem cells (NSCs) and slightly decrease the viability of the C6 glioma, but not of NSCs.
32448603	4	16	theme	neural	746:751	arg1	cells					758:762	neural stem cells	746:762	neural stem cells (NSCs)	746:769	Hydrogels based on low-esterified pectin significantly slow down the metabolism of C6 glioma cells and neural stem cells (NSCs) and slightly decrease the viability of the C6 glioma, but not of NSCs.
32448603	3	17	theme	properties	563:572	arg1	analysis					537:544	the analysis	533:544	the analysis of visco-elastic properties of the gels	533:584	We discovered optimal gelation conditions in the presence of Ca2+ by the analysis of visco-elastic properties of the gels and produced a series of biomaterials in hydrogel forms.
32448603	4	18	theme	NSCs	836:839	arg1	viability					797:805	the viability	793:805	the viability of the C6 glioma, but not of NSCs	793:839	Hydrogels based on low-esterified pectin significantly slow down the metabolism of C6 glioma cells and neural stem cells (NSCs) and slightly decrease the viability of the C6 glioma, but not of NSCs.
32448603	8	19	theme	modeling	1293:1300	arg1	invasion					1314:1321	modeling brain tumor invasion	1293:1321	modeling brain tumor invasion	1293:1321	Thus, composite matrix materials obtained can be used for modeling brain tumor invasion.
32448603	3	20	theme	optimal	478:484	arg1	conditions					495:504	optimal gelation conditions	478:504	optimal gelation conditions in the presence of Ca2+	478:528	We discovered optimal gelation conditions in the presence of Ca2+ by the analysis of visco-elastic properties of the gels and produced a series of biomaterials in hydrogel forms.
32448603	8	21	theme	matrix	1251:1256	arg1	materials					1258:1266	composite matrix materials	1241:1266	composite matrix materials obtained	1241:1275	Thus, composite matrix materials obtained can be used for modeling brain tumor invasion.
32448603	7	22	theme	ECM	1184:1186	arg1	proteins					1188:1195	two ECM proteins	1180:1195	two ECM proteins in gels used for 3D cell cultivation	1180:1232	We have shown that the formation of cell processes in glioma C6 can be regulated by varying the ratio of two ECM proteins in gels used for 3D cell cultivation.
32448603	2	23	dep	obtained	353:360	arg1	Utilizing					300:308	Utilizing	300:308	Utilizing controllable alkaline de-esterification	300:348	Utilizing controllable alkaline de-esterification we obtained pectin preparation with 27.4% esterification degree and used it for bio-artificial matrix production.
32448603	7	24	theme	cell	1217:1220	arg1	cultivation					1222:1232	3D cell cultivation	1214:1232	3D cell cultivation	1214:1232	We have shown that the formation of cell processes in glioma C6 can be regulated by varying the ratio of two ECM proteins in gels used for 3D cell cultivation.
32448603	8	25	used	used	1284:1287	arg2	materials					1258:1266	composite matrix materials	1241:1266	composite matrix materials obtained	1241:1275	Thus, composite matrix materials obtained can be used for modeling brain tumor invasion.
32448603	2	26	theme	alkaline	323:330	arg1	de-esterification					332:348	controllable alkaline de-esterification	310:348	controllable alkaline de-esterification	310:348	Utilizing controllable alkaline de-esterification we obtained pectin preparation with 27.4% esterification degree and used it for bio-artificial matrix production.
32448603	1	27	theme	brain	189:193	arg1	Glioblastoma					134:145	Glioblastoma	134:145	Glioblastoma	134:145	Glioblastoma is the most common malignant tumor of the brain, but its treatment outcomes can be improved by new therapeutic techniques using biocompatible materials.
32448603	1	27	theme	brain	189:193	arg1	tumor					176:180	the most common malignant tumor	150:180	the most common malignant tumor of the brain	150:193	Glioblastoma is the most common malignant tumor of the brain, but its treatment outcomes can be improved by new therapeutic techniques using biocompatible materials.
32448603	0	28	theme	modified	19:26	arg1	pectins					28:34	modified pectins	19:34	modified pectins capable of modulating neural cell behavior as prospective biomaterials in glioblastoma treatment	19:131	Hydrogels based on modified pectins capable of modulating neural cell behavior as prospective biomaterials in glioblastoma treatment.
32448603	1	29	theme	biocompatible	275:287	arg1	materials					289:297	biocompatible materials	275:297	biocompatible materials	275:297	Glioblastoma is the most common malignant tumor of the brain, but its treatment outcomes can be improved by new therapeutic techniques using biocompatible materials.
32448603	8	30	theme	composite	1241:1249	arg1	materials					1258:1266	composite matrix materials	1241:1266	composite matrix materials obtained	1241:1275	Thus, composite matrix materials obtained can be used for modeling brain tumor invasion.
32448603	2	31	theme	controllable	310:321	arg1	de-esterification					332:348	controllable alkaline de-esterification	310:348	controllable alkaline de-esterification	310:348	Utilizing controllable alkaline de-esterification we obtained pectin preparation with 27.4% esterification degree and used it for bio-artificial matrix production.
32448603	4	32	theme	low-esterified	662:675	arg1	pectin					677:682	low-esterified pectin	662:682	low-esterified pectin	662:682	Hydrogels based on low-esterified pectin significantly slow down the metabolism of C6 glioma cells and neural stem cells (NSCs) and slightly decrease the viability of the C6 glioma, but not of NSCs.
32448603	6	33	dep	proteins-collagens	1046:1063	arg1	I					1065:1065	I	1065:1065	I	1065:1065	We created a set of pectin hydrogels supplemented with different ratios of two ECM proteins-collagens I and IV.
32448603	6	33	dep	proteins-collagens	1046:1063	arg1	proteins-collagens					1046:1063	two ECM proteins-collagens I and IV	1038:1072	two ECM proteins-collagens I and IV	1038:1072	We created a set of pectin hydrogels supplemented with different ratios of two ECM proteins-collagens I and IV.
32448603	6	33	dep	proteins-collagens	1046:1063	arg1	IV					1071:1072	IV	1071:1072	IV	1071:1072	We created a set of pectin hydrogels supplemented with different ratios of two ECM proteins-collagens I and IV.
32448603	8	34	theme	brain	1302:1306	arg1	invasion					1314:1321	modeling brain tumor invasion	1293:1321	modeling brain tumor invasion	1293:1321	Thus, composite matrix materials obtained can be used for modeling brain tumor invasion.
32448603	4	35	theme	glioma	729:734	arg1	cells					736:740	C6 glioma cells	726:740	C6 glioma cells	726:740	Hydrogels based on low-esterified pectin significantly slow down the metabolism of C6 glioma cells and neural stem cells (NSCs) and slightly decrease the viability of the C6 glioma, but not of NSCs.
32448603	2	36	theme	matrix	445:450	arg1	production					452:461	bio-artificial matrix production	430:461	bio-artificial matrix production	430:461	Utilizing controllable alkaline de-esterification we obtained pectin preparation with 27.4% esterification degree and used it for bio-artificial matrix production.
32448603	0	37	theme	capable	36:42	arg1	pectins					28:34	modified pectins	19:34	modified pectins capable of modulating neural cell behavior as prospective biomaterials in glioblastoma treatment	19:131	Hydrogels based on modified pectins capable of modulating neural cell behavior as prospective biomaterials in glioblastoma treatment.
32448603	9	38	theme	glioblastoma	1455:1466	arg1	treatment					1468:1476	glioblastoma treatment	1455:1476	glioblastoma treatment due to their ability to regulate glioma cell dynamics	1455:1530	The results presented suggest that modified pectins supplemented with two collagen types may serve as prospective biomaterials for glioblastoma treatment due to their ability to regulate glioma cell dynamics.
32448603	3	39	theme	gelation	486:493	arg1	conditions					495:504	optimal gelation conditions	478:504	optimal gelation conditions in the presence of Ca2+	478:528	We discovered optimal gelation conditions in the presence of Ca2+ by the analysis of visco-elastic properties of the gels and produced a series of biomaterials in hydrogel forms.
32448603	7	40	from	ratio	1171:1175	arg1	gels					1200:1203	gels	1200:1203	gels used for 3D cell cultivation	1200:1232	We have shown that the formation of cell processes in glioma C6 can be regulated by varying the ratio of two ECM proteins in gels used for 3D cell cultivation.
32448603	6	41	theme	proteins-collagens	1046:1063	arg1	ratios					1028:1033	different ratios	1018:1033	different ratios of two ECM proteins-collagens I and IV	1018:1072	We created a set of pectin hydrogels supplemented with different ratios of two ECM proteins-collagens I and IV.
32448603	2	42	theme	bio-artificial	430:443	arg1	production					452:461	bio-artificial matrix production	430:461	bio-artificial matrix production	430:461	Utilizing controllable alkaline de-esterification we obtained pectin preparation with 27.4% esterification degree and used it for bio-artificial matrix production.
32448603	4	43	theme	C6	726:727	arg1	cells					736:740	C6 glioma cells	726:740	C6 glioma cells	726:740	Hydrogels based on low-esterified pectin significantly slow down the metabolism of C6 glioma cells and neural stem cells (NSCs) and slightly decrease the viability of the C6 glioma, but not of NSCs.
32448603	7	44	theme	glioma	1129:1134	arg1	C6					1136:1137	glioma C6	1129:1137	glioma C6	1129:1137	We have shown that the formation of cell processes in glioma C6 can be regulated by varying the ratio of two ECM proteins in gels used for 3D cell cultivation.
32448603	3	45	theme	hydrogel	627:634	arg1	forms					636:640	hydrogel forms	627:640	hydrogel forms	627:640	We discovered optimal gelation conditions in the presence of Ca2+ by the analysis of visco-elastic properties of the gels and produced a series of biomaterials in hydrogel forms.
32448603	9	46	theme	modified	1359:1366	arg1	pectins					1368:1374	modified pectins	1359:1374	modified pectins supplemented with two collagen types	1359:1411	The results presented suggest that modified pectins supplemented with two collagen types may serve as prospective biomaterials for glioblastoma treatment due to their ability to regulate glioma cell dynamics.
32448603	9	46	theme	modified	1359:1366	arg1	biomaterials					1438:1449	prospective biomaterials	1426:1449	prospective biomaterials for glioblastoma treatment due to their ability to regulate glioma cell dynamics	1426:1530	The results presented suggest that modified pectins supplemented with two collagen types may serve as prospective biomaterials for glioblastoma treatment due to their ability to regulate glioma cell dynamics.
32448603	1	47	theme	treatment	204:212	arg1	outcomes					214:221	its treatment outcomes	200:221	its treatment outcomes	200:221	Glioblastoma is the most common malignant tumor of the brain, but its treatment outcomes can be improved by new therapeutic techniques using biocompatible materials.
32448603	7	48	theme	cell	1111:1114	arg1	processes					1116:1124	cell processes	1111:1124	cell processes	1111:1124	We have shown that the formation of cell processes in glioma C6 can be regulated by varying the ratio of two ECM proteins in gels used for 3D cell cultivation.
32448603	7	49	theme	proteins	1188:1195	arg1	ratio					1171:1175	the ratio	1167:1175	the ratio of two ECM proteins in gels used for 3D cell cultivation	1167:1232	We have shown that the formation of cell processes in glioma C6 can be regulated by varying the ratio of two ECM proteins in gels used for 3D cell cultivation.
32448603	9	50	theme	prospective	1426:1436	arg1	pectins					1368:1374	modified pectins	1359:1374	modified pectins supplemented with two collagen types	1359:1411	The results presented suggest that modified pectins supplemented with two collagen types may serve as prospective biomaterials for glioblastoma treatment due to their ability to regulate glioma cell dynamics.
32448603	9	50	theme	prospective	1426:1436	arg1	biomaterials					1438:1449	prospective biomaterials	1426:1449	prospective biomaterials for glioblastoma treatment due to their ability to regulate glioma cell dynamics	1426:1530	The results presented suggest that modified pectins supplemented with two collagen types may serve as prospective biomaterials for glioblastoma treatment due to their ability to regulate glioma cell dynamics.
32448603	3	51	from	conditions	495:504	arg1	presence					513:520	the presence	509:520	the presence of Ca2+	509:528	We discovered optimal gelation conditions in the presence of Ca2+ by the analysis of visco-elastic properties of the gels and produced a series of biomaterials in hydrogel forms.
32448603	3	52	theme	Ca2+	525:528	arg1	presence					513:520	the presence	509:520	the presence of Ca2+	509:528	We discovered optimal gelation conditions in the presence of Ca2+ by the analysis of visco-elastic properties of the gels and produced a series of biomaterials in hydrogel forms.
32448603	7	53	theme	processes	1116:1124	arg1	formation					1098:1106	the formation	1094:1106	the formation of cell processes in glioma C6	1094:1137	We have shown that the formation of cell processes in glioma C6 can be regulated by varying the ratio of two ECM proteins in gels used for 3D cell cultivation.
32448603	7	54	from	formation	1098:1106	arg1	C6					1136:1137	glioma C6	1129:1137	glioma C6	1129:1137	We have shown that the formation of cell processes in glioma C6 can be regulated by varying the ratio of two ECM proteins in gels used for 3D cell cultivation.
32448603	7	55	theme	3D	1214:1215	arg1	cultivation					1222:1232	3D cell cultivation	1214:1232	3D cell cultivation	1214:1232	We have shown that the formation of cell processes in glioma C6 can be regulated by varying the ratio of two ECM proteins in gels used for 3D cell cultivation.
32448603	3	56	theme	biomaterials	611:622	arg1	series					601:606	a series	599:606	a series of biomaterials	599:622	We discovered optimal gelation conditions in the presence of Ca2+ by the analysis of visco-elastic properties of the gels and produced a series of biomaterials in hydrogel forms.
32448603	2	57	theme	pectin	362:367	arg1	preparation					369:379	pectin preparation	362:379	pectin preparation	362:379	Utilizing controllable alkaline de-esterification we obtained pectin preparation with 27.4% esterification degree and used it for bio-artificial matrix production.
32448603	8	58	theme	tumor	1308:1312	arg1	invasion					1314:1321	modeling brain tumor invasion	1293:1321	modeling brain tumor invasion	1293:1321	Thus, composite matrix materials obtained can be used for modeling brain tumor invasion.
32448603	4	59	theme	cells	758:762	arg1	metabolism					712:721	the metabolism	708:721	the metabolism of C6 glioma cells and neural stem cells (NSCs)	708:769	Hydrogels based on low-esterified pectin significantly slow down the metabolism of C6 glioma cells and neural stem cells (NSCs) and slightly decrease the viability of the C6 glioma, but not of NSCs.
32448603	6	60	theme	different	1018:1026	arg1	ratios					1028:1033	different ratios	1018:1033	different ratios of two ECM proteins-collagens I and IV	1018:1072	We created a set of pectin hydrogels supplemented with different ratios of two ECM proteins-collagens I and IV.
32448603	0	61	from	biomaterials	94:105	arg1	treatment					123:131	glioblastoma treatment	110:131	glioblastoma treatment	110:131	Hydrogels based on modified pectins capable of modulating neural cell behavior as prospective biomaterials in glioblastoma treatment.
32448603	3	62	theme	visco-elastic	549:561	arg1	properties					563:572	visco-elastic properties	549:572	visco-elastic properties of the gels	549:584	We discovered optimal gelation conditions in the presence of Ca2+ by the analysis of visco-elastic properties of the gels and produced a series of biomaterials in hydrogel forms.
32448603	5	63	theme	apoptosis	907:915	arg1	degrees					917:923	apoptosis degrees	907:923	apoptosis degrees	907:923	This happens due to a decrease in cell proliferation rate, while apoptosis degrees remain stable or negligibly decrease.
32448603	0	64	theme	cell	65:68	arg1	behavior					70:77	neural cell behavior	58:77	neural cell behavior	58:77	Hydrogels based on modified pectins capable of modulating neural cell behavior as prospective biomaterials in glioblastoma treatment.
32448603	0	64	theme	cell	65:68	arg1	biomaterials					94:105	prospective biomaterials	82:105	prospective biomaterials in glioblastoma treatment	82:131	Hydrogels based on modified pectins capable of modulating neural cell behavior as prospective biomaterials in glioblastoma treatment.
32448603	7	65	from	proteins	1188:1195	arg1	gels					1200:1203	gels	1200:1203	gels used for 3D cell cultivation	1200:1232	We have shown that the formation of cell processes in glioma C6 can be regulated by varying the ratio of two ECM proteins in gels used for 3D cell cultivation.
32448603	4	66	theme	glioma	817:822	arg1	viability					797:805	the viability	793:805	the viability of the C6 glioma, but not of NSCs	793:839	Hydrogels based on low-esterified pectin significantly slow down the metabolism of C6 glioma cells and neural stem cells (NSCs) and slightly decrease the viability of the C6 glioma, but not of NSCs.
32448603	5	67	from	decrease	864:871	arg1	rate					895:898	cell proliferation rate	876:898	cell proliferation rate	876:898	This happens due to a decrease in cell proliferation rate, while apoptosis degrees remain stable or negligibly decrease.
32448603	2	68	theme	esterification	392:405	arg1	degree					407:412	27.4% esterification degree	386:412	27.4% esterification degree	386:412	Utilizing controllable alkaline de-esterification we obtained pectin preparation with 27.4% esterification degree and used it for bio-artificial matrix production.
32448603	9	69	theme	collagen	1398:1405	arg1	types					1407:1411	two collagen types	1394:1411	two collagen types	1394:1411	The results presented suggest that modified pectins supplemented with two collagen types may serve as prospective biomaterials for glioblastoma treatment due to their ability to regulate glioma cell dynamics.
32448603	9	70	theme	glioma	1511:1516	arg1	dynamics					1523:1530	glioma cell dynamics	1511:1530	glioma cell dynamics	1511:1530	The results presented suggest that modified pectins supplemented with two collagen types may serve as prospective biomaterials for glioblastoma treatment due to their ability to regulate glioma cell dynamics.
32448603	0	71	theme	neural	58:63	arg1	behavior					70:77	neural cell behavior	58:77	neural cell behavior	58:77	Hydrogels based on modified pectins capable of modulating neural cell behavior as prospective biomaterials in glioblastoma treatment.
32448603	0	71	theme	neural	58:63	arg1	biomaterials					94:105	prospective biomaterials	82:105	prospective biomaterials in glioblastoma treatment	82:131	Hydrogels based on modified pectins capable of modulating neural cell behavior as prospective biomaterials in glioblastoma treatment.
32448603	4	72	theme	C6	814:815	arg1	glioma					817:822	the C6 glioma	810:822	the C6 glioma	810:822	Hydrogels based on low-esterified pectin significantly slow down the metabolism of C6 glioma cells and neural stem cells (NSCs) and slightly decrease the viability of the C6 glioma, but not of NSCs.
32448603	2	73	used	used	418:421	arg2	we					350:351	we	350:351	we	350:351	Utilizing controllable alkaline de-esterification we obtained pectin preparation with 27.4% esterification degree and used it for bio-artificial matrix production.
32448603	2	74	theme	%	390:390	arg1	degree					407:412	27.4% esterification degree	386:412	27.4% esterification degree	386:412	Utilizing controllable alkaline de-esterification we obtained pectin preparation with 27.4% esterification degree and used it for bio-artificial matrix production.
32448603	6	75	theme	hydrogels	990:998	arg1	set					976:978	a set	974:978	a set of pectin hydrogels supplemented with different ratios of two ECM proteins-collagens I and IV	974:1072	We created a set of pectin hydrogels supplemented with different ratios of two ECM proteins-collagens I and IV.
32448603	6	75	theme	hydrogels	990:998	arg1	hydrogels					990:998	pectin hydrogels	983:998	pectin hydrogels supplemented with different ratios of two ECM proteins-collagens I and IV	983:1072	We created a set of pectin hydrogels supplemented with different ratios of two ECM proteins-collagens I and IV.
34597329	7	0	theme	soil	1238:1241	arg1	content					1252:1258	soil moisture content	1238:1258	soil moisture content	1238:1258	We confirmed that soil available nitrogen, soil pH and soil moisture content significantly affected the fungal community distribution of S. psammophila sand barriers.
34597329	2	1	theme	desert	334:339	arg1	case					308:311	the case	304:311	the case of an extremely arid desert	304:339	In the case of an extremely arid desert, as opposed to forests, little of known about the fungal community composition of decaying wood and the effects of decomposing wood on soil physical and chemical properties.
34597329	9	2	theme	interactions	1602:1613	arg1	importance					1574:1583	the importance	1570:1583	the importance of bidirectional interactions between fungi in decayed sand barriers and soil properties	1570:1672	Although our results highlighted the importance of bidirectional interactions between fungi in decayed sand barriers and soil properties, their contribution to the desert ecosystem still needs further confirmation from future studies.
34597329	3	3	theme	barriers	739:746	arg1	decomposition					699:711	the decomposition	695:711	the decomposition of Salix psammophila sand barriers	695:746	Combined with high-throughput gene sequencing technology, we investigated the relationships between microenvironment factors with fungal community composition and diversity during the decomposition of Salix psammophila sand barriers.
34597329	1	4	theme	nutrient	266:273	arg1	dynamics					275:282	nutrient dynamics	266:282	nutrient dynamics in desert soils	266:298	Wood-inhabiting fungi are crucial to wood decay and decomposition in S. psammophila sand barriers, which in turn consumingly influence nutrient dynamics in desert soils.
34597329	4	5	theme	barrier	866:872	arg1	process					835:841	the decay process	825:841	the decay process of S. psammophila sand barrier	825:872	The results showed that the destruction of lignocellulose components during the decay process of S. psammophila sand barrier alters the physical and chemical properties of the surrounding soil.
34597329	3	6	theme	psammophila	722:732	arg1	barriers					739:746	Salix psammophila sand barriers	716:746	Salix psammophila sand barriers	716:746	Combined with high-throughput gene sequencing technology, we investigated the relationships between microenvironment factors with fungal community composition and diversity during the decomposition of Salix psammophila sand barriers.
34597329	3	7	theme	sequencing	550:559	arg1	technology					561:570	high-throughput gene sequencing technology	529:570	high-throughput gene sequencing technology	529:570	Combined with high-throughput gene sequencing technology, we investigated the relationships between microenvironment factors with fungal community composition and diversity during the decomposition of Salix psammophila sand barriers.
34597329	3	8	with	factors	632:638	arg1	diversity					678:686	diversity	678:686	diversity	678:686	Combined with high-throughput gene sequencing technology, we investigated the relationships between microenvironment factors with fungal community composition and diversity during the decomposition of Salix psammophila sand barriers.
34597329	3	8	with	factors	632:638	arg1	composition					662:672	fungal community composition	645:672	fungal community composition	645:672	Combined with high-throughput gene sequencing technology, we investigated the relationships between microenvironment factors with fungal community composition and diversity during the decomposition of Salix psammophila sand barriers.
34597329	3	9	theme	high-throughput	529:543	arg1	technology					561:570	high-throughput gene sequencing technology	529:570	high-throughput gene sequencing technology	529:570	Combined with high-throughput gene sequencing technology, we investigated the relationships between microenvironment factors with fungal community composition and diversity during the decomposition of Salix psammophila sand barriers.
34597329	1	10	theme	S.	200:201	arg1	barriers					220:227	S. psammophila sand barriers	200:227	S. psammophila sand barriers	200:227	Wood-inhabiting fungi are crucial to wood decay and decomposition in S. psammophila sand barriers, which in turn consumingly influence nutrient dynamics in desert soils.
34597329	9	11	theme	future	1756:1761	arg1	studies					1763:1769	future studies	1756:1769	future studies	1756:1769	Although our results highlighted the importance of bidirectional interactions between fungi in decayed sand barriers and soil properties, their contribution to the desert ecosystem still needs further confirmation from future studies.
34597329	2	12	from	effects	445:451	arg1	properties					503:512	soil physical and chemical properties	476:512	soil physical and chemical properties	476:512	In the case of an extremely arid desert, as opposed to forests, little of known about the fungal community composition of decaying wood and the effects of decomposing wood on soil physical and chemical properties.
34597329	10	13	theme	interactions	1862:1873	arg1	understanding					1823:1835	the current understanding	1811:1835	the current understanding of the sand barrier-soil interactions	1811:1873	However, overall our findings improved the current understanding of the sand barrier-soil interactions on the process of ecological restoration.
34597329	1	14	theme	sand	215:218	arg1	barriers					220:227	S. psammophila sand barriers	200:227	S. psammophila sand barriers	200:227	Wood-inhabiting fungi are crucial to wood decay and decomposition in S. psammophila sand barriers, which in turn consumingly influence nutrient dynamics in desert soils.
34597329	8	15	theme	positive	1472:1479	arg1	correlation					1481:1491	the positive correlation	1468:1491	the positive correlation of soil moisture content and soil porosity	1468:1534	Sordariomycetes are mainly affected by the positive correlation of soil pH, while Eurotiomycetes are most affected by the positive correlation of soil moisture content and soil porosity.
34597329	10	16	theme	sand	1844:1847	arg1	interactions					1862:1873	the sand barrier-soil interactions	1840:1873	the sand barrier-soil interactions	1840:1873	However, overall our findings improved the current understanding of the sand barrier-soil interactions on the process of ecological restoration.
34597329	4	17	theme	soil	937:940	arg1	properties					907:916	the physical and chemical properties	881:916	the physical and chemical properties of the surrounding soil	881:940	The results showed that the destruction of lignocellulose components during the decay process of S. psammophila sand barrier alters the physical and chemical properties of the surrounding soil.
34597329	3	18	theme	microenvironment	615:630	arg1	factors					632:638	microenvironment factors	615:638	microenvironment factors with fungal community composition and diversity	615:686	Combined with high-throughput gene sequencing technology, we investigated the relationships between microenvironment factors with fungal community composition and diversity during the decomposition of Salix psammophila sand barriers.
34597329	5	19	theme	one-year	957:964	arg1	barrier					971:977	one-year sand barrier	957:977	one-year sand barrier	957:977	Compared with one-year sand barrier, lignin and cellulose of seven-year S. psammophila sand barrier decreased by 40.48% and 38.33%, respectively.
34597329	2	20	theme	wood	468:471	arg1	wood					432:435	decaying wood	423:435	decaying wood	423:435	In the case of an extremely arid desert, as opposed to forests, little of known about the fungal community composition of decaying wood and the effects of decomposing wood on soil physical and chemical properties.
34597329	2	20	theme	wood	468:471	arg1	effects					445:451	the effects	441:451	the effects of decomposing wood on soil physical and chemical properties	441:512	In the case of an extremely arid desert, as opposed to forests, little of known about the fungal community composition of decaying wood and the effects of decomposing wood on soil physical and chemical properties.
34597329	8	21	theme	moisture	1501:1508	arg1	content					1510:1516	soil moisture content	1496:1516	soil moisture content	1496:1516	Sordariomycetes are mainly affected by the positive correlation of soil pH, while Eurotiomycetes are most affected by the positive correlation of soil moisture content and soil porosity.
34597329	0	22	theme	decaying	64:71	arg1	process					73:79	the decaying process	60:79	the decaying process of Salix psammophila sand barriers in the desert	60:128	Fungal community characteristics and driving factors during the decaying process of Salix psammophila sand barriers in the desert.
34597329	7	23	theme	soil	1226:1229	arg1	pH					1231:1232	soil pH	1226:1232	soil pH	1226:1232	We confirmed that soil available nitrogen, soil pH and soil moisture content significantly affected the fungal community distribution of S. psammophila sand barriers.
34597329	9	24	theme	desert	1701:1706	arg1	ecosystem					1708:1716	the desert ecosystem	1697:1716	the desert ecosystem	1697:1716	Although our results highlighted the importance of bidirectional interactions between fungi in decayed sand barriers and soil properties, their contribution to the desert ecosystem still needs further confirmation from future studies.
34597329	9	25	from	studies	1763:1769	arg1	confirmation					1738:1749	further confirmation	1730:1749	further confirmation from future studies	1730:1769	Although our results highlighted the importance of bidirectional interactions between fungi in decayed sand barriers and soil properties, their contribution to the desert ecosystem still needs further confirmation from future studies.
34597329	2	26	from	composition	408:418	arg1	properties					503:512	soil physical and chemical properties	476:512	soil physical and chemical properties	476:512	In the case of an extremely arid desert, as opposed to forests, little of known about the fungal community composition of decaying wood and the effects of decomposing wood on soil physical and chemical properties.
34597329	0	27	theme	psammophila	90:100	arg1	barriers					107:114	Salix psammophila sand barriers	84:114	Salix psammophila sand barriers	84:114	Fungal community characteristics and driving factors during the decaying process of Salix psammophila sand barriers in the desert.
34597329	1	28	theme	Wood-inhabiting	131:145	arg1	fungi					147:151	Wood-inhabiting fungi	131:151	Wood-inhabiting fungi	131:151	Wood-inhabiting fungi are crucial to wood decay and decomposition in S. psammophila sand barriers, which in turn consumingly influence nutrient dynamics in desert soils.
34597329	2	29	theme	soil	476:479	arg1	properties					503:512	soil physical and chemical properties	476:512	soil physical and chemical properties	476:512	In the case of an extremely arid desert, as opposed to forests, little of known about the fungal community composition of decaying wood and the effects of decomposing wood on soil physical and chemical properties.
34597329	2	30	dep	opposed	345:351	arg1	case					308:311	the case	304:311	the case of an extremely arid desert	304:339	In the case of an extremely arid desert, as opposed to forests, little of known about the fungal community composition of decaying wood and the effects of decomposing wood on soil physical and chemical properties.
34597329	0	31	theme	barriers	107:114	arg1	process					73:79	the decaying process	60:79	the decaying process of Salix psammophila sand barriers in the desert	60:128	Fungal community characteristics and driving factors during the decaying process of Salix psammophila sand barriers in the desert.
34597329	5	32	theme	seven-year	1004:1013	arg1	barrier					1035:1041	seven-year S. psammophila sand barrier	1004:1041	seven-year S. psammophila sand barrier	1004:1041	Compared with one-year sand barrier, lignin and cellulose of seven-year S. psammophila sand barrier decreased by 40.48% and 38.33%, respectively.
34597329	6	33	theme	Soil	1089:1092	arg1	potassium					1104:1112	Soil available potassium	1089:1112	Soil available potassium	1089:1112	Soil available potassium and available nitrogen increased by 39.80% and 99.46%, respectively.
34597329	8	34	theme	positive	1393:1400	arg1	correlation					1402:1412	the positive correlation	1389:1412	the positive correlation of soil pH	1389:1423	Sordariomycetes are mainly affected by the positive correlation of soil pH, while Eurotiomycetes are most affected by the positive correlation of soil moisture content and soil porosity.
34597329	0	35	theme	Fungal	0:5	arg1	characteristics					17:31	Fungal community characteristics	0:31	Fungal community characteristics	0:31	Fungal community characteristics and driving factors during the decaying process of Salix psammophila sand barriers in the desert.
34597329	7	36	theme	barriers	1340:1347	arg1	distribution					1304:1315	the fungal community distribution	1283:1315	the fungal community distribution of S. psammophila sand barriers	1283:1347	We confirmed that soil available nitrogen, soil pH and soil moisture content significantly affected the fungal community distribution of S. psammophila sand barriers.
34597329	2	37	theme	community	398:406	arg1	composition					408:418	the fungal community composition	387:418	the fungal community composition of decaying wood and the effects of decomposing wood on soil physical and chemical properties	387:512	In the case of an extremely arid desert, as opposed to forests, little of known about the fungal community composition of decaying wood and the effects of decomposing wood on soil physical and chemical properties.
34597329	2	38	dep	known	375:379	arg1	little					365:370	little	365:370	little	365:370	In the case of an extremely arid desert, as opposed to forests, little of known about the fungal community composition of decaying wood and the effects of decomposing wood on soil physical and chemical properties.
34597329	1	39	theme	desert	287:292	arg1	soils					294:298	desert soils	287:298	desert soils	287:298	Wood-inhabiting fungi are crucial to wood decay and decomposition in S. psammophila sand barriers, which in turn consumingly influence nutrient dynamics in desert soils.
34597329	7	40	theme	psammophila	1323:1333	arg1	barriers					1340:1347	S. psammophila sand barriers	1320:1347	S. psammophila sand barriers	1320:1347	We confirmed that soil available nitrogen, soil pH and soil moisture content significantly affected the fungal community distribution of S. psammophila sand barriers.
34597329	3	41	theme	community	652:660	arg1	composition					662:672	fungal community composition	645:672	fungal community composition	645:672	Combined with high-throughput gene sequencing technology, we investigated the relationships between microenvironment factors with fungal community composition and diversity during the decomposition of Salix psammophila sand barriers.
34597329	8	42	theme	pH	1422:1423	arg1	correlation					1402:1412	the positive correlation	1389:1412	the positive correlation of soil pH	1389:1423	Sordariomycetes are mainly affected by the positive correlation of soil pH, while Eurotiomycetes are most affected by the positive correlation of soil moisture content and soil porosity.
34597329	0	43	theme	driving	37:43	arg1	factors					45:51	driving factors	37:51	driving factors	37:51	Fungal community characteristics and driving factors during the decaying process of Salix psammophila sand barriers in the desert.
34597329	9	44	theme	sand	1640:1643	arg1	barriers					1645:1652	decayed sand barriers	1632:1652	decayed sand barriers	1632:1652	Although our results highlighted the importance of bidirectional interactions between fungi in decayed sand barriers and soil properties, their contribution to the desert ecosystem still needs further confirmation from future studies.
34597329	2	45	theme	wood	432:435	arg1	composition					408:418	the fungal community composition	387:418	the fungal community composition of decaying wood and the effects of decomposing wood on soil physical and chemical properties	387:512	In the case of an extremely arid desert, as opposed to forests, little of known about the fungal community composition of decaying wood and the effects of decomposing wood on soil physical and chemical properties.
34597329	10	46	theme	restoration	1904:1914	arg1	process					1882:1888	the process	1878:1888	the process of ecological restoration	1878:1914	However, overall our findings improved the current understanding of the sand barrier-soil interactions on the process of ecological restoration.
34597329	4	47	theme	psammophila	849:859	arg1	barrier					866:872	S. psammophila sand barrier	846:872	S. psammophila sand barrier	846:872	The results showed that the destruction of lignocellulose components during the decay process of S. psammophila sand barrier alters the physical and chemical properties of the surrounding soil.
34597329	2	48	theme	arid	329:332	arg1	desert					334:339	an extremely arid desert	316:339	an extremely arid desert	316:339	In the case of an extremely arid desert, as opposed to forests, little of known about the fungal community composition of decaying wood and the effects of decomposing wood on soil physical and chemical properties.
34597329	7	49	theme	community	1294:1302	arg1	distribution					1304:1315	the fungal community distribution	1283:1315	the fungal community distribution of S. psammophila sand barriers	1283:1347	We confirmed that soil available nitrogen, soil pH and soil moisture content significantly affected the fungal community distribution of S. psammophila sand barriers.
34597329	5	50	theme	psammophila	1018:1028	arg1	barrier					1035:1041	seven-year S. psammophila sand barrier	1004:1041	seven-year S. psammophila sand barrier	1004:1041	Compared with one-year sand barrier, lignin and cellulose of seven-year S. psammophila sand barrier decreased by 40.48% and 38.33%, respectively.
34597329	4	51	theme	decay	829:833	arg1	process					835:841	the decay process	825:841	the decay process of S. psammophila sand barrier	825:872	The results showed that the destruction of lignocellulose components during the decay process of S. psammophila sand barrier alters the physical and chemical properties of the surrounding soil.
34597329	5	52	theme	barrier	1035:1041	arg1	cellulose					991:999	cellulose	991:999	cellulose	991:999	Compared with one-year sand barrier, lignin and cellulose of seven-year S. psammophila sand barrier decreased by 40.48% and 38.33%, respectively.
34597329	5	52	theme	barrier	1035:1041	arg1	lignin					980:985	lignin	980:985	lignin	980:985	Compared with one-year sand barrier, lignin and cellulose of seven-year S. psammophila sand barrier decreased by 40.48% and 38.33%, respectively.
34597329	6	53	theme	available	1118:1126	arg1	nitrogen					1128:1135	available nitrogen	1118:1135	available nitrogen	1118:1135	Soil available potassium and available nitrogen increased by 39.80% and 99.46%, respectively.
34597329	3	54	theme	Salix	716:720	arg1	barriers					739:746	Salix psammophila sand barriers	716:746	Salix psammophila sand barriers	716:746	Combined with high-throughput gene sequencing technology, we investigated the relationships between microenvironment factors with fungal community composition and diversity during the decomposition of Salix psammophila sand barriers.
34597329	4	55	theme	physical	885:892	arg1	properties					907:916	the physical and chemical properties	881:916	the physical and chemical properties of the surrounding soil	881:940	The results showed that the destruction of lignocellulose components during the decay process of S. psammophila sand barrier alters the physical and chemical properties of the surrounding soil.
34597329	7	56	theme	moisture	1243:1250	arg1	content					1252:1258	soil moisture content	1238:1258	soil moisture content	1238:1258	We confirmed that soil available nitrogen, soil pH and soil moisture content significantly affected the fungal community distribution of S. psammophila sand barriers.
34597329	4	57	theme	chemical	898:905	arg1	properties					907:916	the physical and chemical properties	881:916	the physical and chemical properties of the surrounding soil	881:940	The results showed that the destruction of lignocellulose components during the decay process of S. psammophila sand barrier alters the physical and chemical properties of the surrounding soil.
34597329	1	58	theme	wood	168:171	arg1	decay					173:177	wood decay	168:177	wood decay	168:177	Wood-inhabiting fungi are crucial to wood decay and decomposition in S. psammophila sand barriers, which in turn consumingly influence nutrient dynamics in desert soils.
34597329	4	59	theme	sand	861:864	arg1	barrier					866:872	S. psammophila sand barrier	846:872	S. psammophila sand barrier	846:872	The results showed that the destruction of lignocellulose components during the decay process of S. psammophila sand barrier alters the physical and chemical properties of the surrounding soil.
34597329	3	60	theme	sand	734:737	arg1	barriers					739:746	Salix psammophila sand barriers	716:746	Salix psammophila sand barriers	716:746	Combined with high-throughput gene sequencing technology, we investigated the relationships between microenvironment factors with fungal community composition and diversity during the decomposition of Salix psammophila sand barriers.
34597329	1	61	from	dynamics	275:282	arg1	soils					294:298	desert soils	287:298	desert soils	287:298	Wood-inhabiting fungi are crucial to wood decay and decomposition in S. psammophila sand barriers, which in turn consumingly influence nutrient dynamics in desert soils.
34597329	3	62	theme	gene	545:548	arg1	technology					561:570	high-throughput gene sequencing technology	529:570	high-throughput gene sequencing technology	529:570	Combined with high-throughput gene sequencing technology, we investigated the relationships between microenvironment factors with fungal community composition and diversity during the decomposition of Salix psammophila sand barriers.
34597329	9	63	theme	further	1730:1736	arg1	confirmation					1738:1749	further confirmation	1730:1749	further confirmation from future studies	1730:1769	Although our results highlighted the importance of bidirectional interactions between fungi in decayed sand barriers and soil properties, their contribution to the desert ecosystem still needs further confirmation from future studies.
34597329	1	64	theme	psammophila	203:213	arg1	barriers					220:227	S. psammophila sand barriers	200:227	S. psammophila sand barriers	200:227	Wood-inhabiting fungi are crucial to wood decay and decomposition in S. psammophila sand barriers, which in turn consumingly influence nutrient dynamics in desert soils.
34597329	2	65	theme	chemical	494:501	arg1	properties					503:512	soil physical and chemical properties	476:512	soil physical and chemical properties	476:512	In the case of an extremely arid desert, as opposed to forests, little of known about the fungal community composition of decaying wood and the effects of decomposing wood on soil physical and chemical properties.
34597329	0	66	from	process	73:79	arg1	desert					123:128	the desert	119:128	the desert	119:128	Fungal community characteristics and driving factors during the decaying process of Salix psammophila sand barriers in the desert.
34597329	7	67	theme	available	1206:1214	arg1	nitrogen					1216:1223	soil available nitrogen	1201:1223	soil available nitrogen	1201:1223	We confirmed that soil available nitrogen, soil pH and soil moisture content significantly affected the fungal community distribution of S. psammophila sand barriers.
34597329	10	68	theme	barrier-soil	1849:1860	arg1	interactions					1862:1873	the sand barrier-soil interactions	1840:1873	the sand barrier-soil interactions	1840:1873	However, overall our findings improved the current understanding of the sand barrier-soil interactions on the process of ecological restoration.
34597329	8	69	theme	porosity	1527:1534	arg1	correlation					1481:1491	the positive correlation	1468:1491	the positive correlation of soil moisture content and soil porosity	1468:1534	Sordariomycetes are mainly affected by the positive correlation of soil pH, while Eurotiomycetes are most affected by the positive correlation of soil moisture content and soil porosity.
34597329	8	70	theme	soil	1496:1499	arg1	content					1510:1516	soil moisture content	1496:1516	soil moisture content	1496:1516	Sordariomycetes are mainly affected by the positive correlation of soil pH, while Eurotiomycetes are most affected by the positive correlation of soil moisture content and soil porosity.
34597329	10	71	theme	current	1815:1821	arg1	understanding					1823:1835	the current understanding	1811:1835	the current understanding of the sand barrier-soil interactions	1811:1873	However, overall our findings improved the current understanding of the sand barrier-soil interactions on the process of ecological restoration.
34597329	9	72	theme	soil	1658:1661	arg1	properties					1663:1672	soil properties	1658:1672	soil properties	1658:1672	Although our results highlighted the importance of bidirectional interactions between fungi in decayed sand barriers and soil properties, their contribution to the desert ecosystem still needs further confirmation from future studies.
34597329	10	73	theme	ecological	1893:1902	arg1	restoration					1904:1914	ecological restoration	1893:1914	ecological restoration	1893:1914	However, overall our findings improved the current understanding of the sand barrier-soil interactions on the process of ecological restoration.
34597329	4	74	theme	surrounding	925:935	arg1	soil					937:940	the surrounding soil	921:940	the surrounding soil	921:940	The results showed that the destruction of lignocellulose components during the decay process of S. psammophila sand barrier alters the physical and chemical properties of the surrounding soil.
34597329	8	75	theme	content	1510:1516	arg1	correlation					1481:1491	the positive correlation	1468:1491	the positive correlation of soil moisture content and soil porosity	1468:1534	Sordariomycetes are mainly affected by the positive correlation of soil pH, while Eurotiomycetes are most affected by the positive correlation of soil moisture content and soil porosity.
34597329	1	76	from	decomposition	183:195	arg1	barriers					220:227	S. psammophila sand barriers	200:227	S. psammophila sand barriers	200:227	Wood-inhabiting fungi are crucial to wood decay and decomposition in S. psammophila sand barriers, which in turn consumingly influence nutrient dynamics in desert soils.
34597329	2	77	theme	decomposing	456:466	arg1	wood					468:471	decomposing wood	456:471	decomposing wood	456:471	In the case of an extremely arid desert, as opposed to forests, little of known about the fungal community composition of decaying wood and the effects of decomposing wood on soil physical and chemical properties.
34597329	8	78	theme	soil	1522:1525	arg1	porosity					1527:1534	soil porosity	1522:1534	soil porosity	1522:1534	Sordariomycetes are mainly affected by the positive correlation of soil pH, while Eurotiomycetes are most affected by the positive correlation of soil moisture content and soil porosity.
34597329	0	79	theme	Salix	84:88	arg1	barriers					107:114	Salix psammophila sand barriers	84:114	Salix psammophila sand barriers	84:114	Fungal community characteristics and driving factors during the decaying process of Salix psammophila sand barriers in the desert.
34597329	1	80	from	decay	173:177	arg1	barriers					220:227	S. psammophila sand barriers	200:227	S. psammophila sand barriers	200:227	Wood-inhabiting fungi are crucial to wood decay and decomposition in S. psammophila sand barriers, which in turn consumingly influence nutrient dynamics in desert soils.
34597329	2	81	theme	effects	445:451	arg1	composition					408:418	the fungal community composition	387:418	the fungal community composition of decaying wood and the effects of decomposing wood on soil physical and chemical properties	387:512	In the case of an extremely arid desert, as opposed to forests, little of known about the fungal community composition of decaying wood and the effects of decomposing wood on soil physical and chemical properties.
34597329	0	82	theme	sand	102:105	arg1	barriers					107:114	Salix psammophila sand barriers	84:114	Salix psammophila sand barriers	84:114	Fungal community characteristics and driving factors during the decaying process of Salix psammophila sand barriers in the desert.
34597329	9	83	from	importance	1574:1583	arg1	barriers					1645:1652	decayed sand barriers	1632:1652	decayed sand barriers	1632:1652	Although our results highlighted the importance of bidirectional interactions between fungi in decayed sand barriers and soil properties, their contribution to the desert ecosystem still needs further confirmation from future studies.
34597329	9	83	from	importance	1574:1583	arg1	properties					1663:1672	soil properties	1658:1672	soil properties	1658:1672	Although our results highlighted the importance of bidirectional interactions between fungi in decayed sand barriers and soil properties, their contribution to the desert ecosystem still needs further confirmation from future studies.
34597329	2	84	theme	physical	481:488	arg1	properties					503:512	soil physical and chemical properties	476:512	soil physical and chemical properties	476:512	In the case of an extremely arid desert, as opposed to forests, little of known about the fungal community composition of decaying wood and the effects of decomposing wood on soil physical and chemical properties.
34597329	2	85	from	wood	432:435	arg1	properties					503:512	soil physical and chemical properties	476:512	soil physical and chemical properties	476:512	In the case of an extremely arid desert, as opposed to forests, little of known about the fungal community composition of decaying wood and the effects of decomposing wood on soil physical and chemical properties.
34597329	6	86	theme	available	1094:1102	arg1	potassium					1104:1112	Soil available potassium	1089:1112	Soil available potassium	1089:1112	Soil available potassium and available nitrogen increased by 39.80% and 99.46%, respectively.
34597329	0	87	theme	community	7:15	arg1	characteristics					17:31	Fungal community characteristics	0:31	Fungal community characteristics	0:31	Fungal community characteristics and driving factors during the decaying process of Salix psammophila sand barriers in the desert.
34597329	5	88	theme	sand	966:969	arg1	barrier					971:977	one-year sand barrier	957:977	one-year sand barrier	957:977	Compared with one-year sand barrier, lignin and cellulose of seven-year S. psammophila sand barrier decreased by 40.48% and 38.33%, respectively.
34597329	7	89	theme	sand	1335:1338	arg1	barriers					1340:1347	S. psammophila sand barriers	1320:1347	S. psammophila sand barriers	1320:1347	We confirmed that soil available nitrogen, soil pH and soil moisture content significantly affected the fungal community distribution of S. psammophila sand barriers.
34597329	7	90	theme	S.	1320:1321	arg1	barriers					1340:1347	S. psammophila sand barriers	1320:1347	S. psammophila sand barriers	1320:1347	We confirmed that soil available nitrogen, soil pH and soil moisture content significantly affected the fungal community distribution of S. psammophila sand barriers.
34597329	2	91	theme	fungal	391:396	arg1	composition					408:418	the fungal community composition	387:418	the fungal community composition of decaying wood and the effects of decomposing wood on soil physical and chemical properties	387:512	In the case of an extremely arid desert, as opposed to forests, little of known about the fungal community composition of decaying wood and the effects of decomposing wood on soil physical and chemical properties.
34597329	4	92	theme	lignocellulose	792:805	arg1	components					807:816	lignocellulose components	792:816	lignocellulose components	792:816	The results showed that the destruction of lignocellulose components during the decay process of S. psammophila sand barrier alters the physical and chemical properties of the surrounding soil.
34597329	3	93	theme	fungal	645:650	arg1	composition					662:672	fungal community composition	645:672	fungal community composition	645:672	Combined with high-throughput gene sequencing technology, we investigated the relationships between microenvironment factors with fungal community composition and diversity during the decomposition of Salix psammophila sand barriers.
34597329	9	94	theme	decayed	1632:1638	arg1	barriers					1645:1652	decayed sand barriers	1632:1652	decayed sand barriers	1632:1652	Although our results highlighted the importance of bidirectional interactions between fungi in decayed sand barriers and soil properties, their contribution to the desert ecosystem still needs further confirmation from future studies.
34597329	8	95	theme	soil	1417:1420	arg1	pH					1422:1423	soil pH	1417:1423	soil pH	1417:1423	Sordariomycetes are mainly affected by the positive correlation of soil pH, while Eurotiomycetes are most affected by the positive correlation of soil moisture content and soil porosity.
34597329	7	96	theme	soil	1201:1204	arg1	nitrogen					1216:1223	soil available nitrogen	1201:1223	soil available nitrogen	1201:1223	We confirmed that soil available nitrogen, soil pH and soil moisture content significantly affected the fungal community distribution of S. psammophila sand barriers.
34597329	7	97	theme	fungal	1287:1292	arg1	distribution					1304:1315	the fungal community distribution	1283:1315	the fungal community distribution of S. psammophila sand barriers	1283:1347	We confirmed that soil available nitrogen, soil pH and soil moisture content significantly affected the fungal community distribution of S. psammophila sand barriers.
34597329	2	98	theme	decaying	423:430	arg1	wood					432:435	decaying wood	423:435	decaying wood	423:435	In the case of an extremely arid desert, as opposed to forests, little of known about the fungal community composition of decaying wood and the effects of decomposing wood on soil physical and chemical properties.
34597329	4	99	theme	S.	846:847	arg1	barrier					866:872	S. psammophila sand barrier	846:872	S. psammophila sand barrier	846:872	The results showed that the destruction of lignocellulose components during the decay process of S. psammophila sand barrier alters the physical and chemical properties of the surrounding soil.
34597329	5	100	theme	S.	1015:1016	arg1	barrier					1035:1041	seven-year S. psammophila sand barrier	1004:1041	seven-year S. psammophila sand barrier	1004:1041	Compared with one-year sand barrier, lignin and cellulose of seven-year S. psammophila sand barrier decreased by 40.48% and 38.33%, respectively.
34597329	2	101	from	properties	503:512	arg1	composition					408:418	the fungal community composition	387:418	the fungal community composition of decaying wood and the effects of decomposing wood on soil physical and chemical properties	387:512	In the case of an extremely arid desert, as opposed to forests, little of known about the fungal community composition of decaying wood and the effects of decomposing wood on soil physical and chemical properties.
34597329	9	102	theme	bidirectional	1588:1600	arg1	interactions					1602:1613	bidirectional interactions	1588:1613	bidirectional interactions between fungi	1588:1627	Although our results highlighted the importance of bidirectional interactions between fungi in decayed sand barriers and soil properties, their contribution to the desert ecosystem still needs further confirmation from future studies.
34597329	4	103	theme	components	807:816	arg1	destruction					777:787	the destruction	773:787	the destruction of lignocellulose components during the decay process of S. psammophila sand barrier	773:872	The results showed that the destruction of lignocellulose components during the decay process of S. psammophila sand barrier alters the physical and chemical properties of the surrounding soil.
34597329	5	104	theme	sand	1030:1033	arg1	barrier					1035:1041	seven-year S. psammophila sand barrier	1004:1041	seven-year S. psammophila sand barrier	1004:1041	Compared with one-year sand barrier, lignin and cellulose of seven-year S. psammophila sand barrier decreased by 40.48% and 38.33%, respectively.
34752210	6	0	with	cluster	749:755	arg1	RQJ05T					778:783	A. agarilyticus RQJ05T	762:783	A. agarilyticus RQJ05T	762:783	Strain CCB-QB4T formed a cluster with A. agarilyticus RQJ05T.
34752210	8	1	theme	NaCl	972:975	arg1	presence					948:955	the presence	944:955	the presence of 1-6 % (w/v) NaCl and glucose	944:987	Cell growth was observed at 15-40 °C, at pH 7.0-10.0 and in the presence of 1-6 % (w/v) NaCl and glucose.
34752210	5	2	theme	strain	510:515	arg1	CCB-QB4T					517:524	strain CCB-QB4T	510:524	strain CCB-QB4T	510:524	Phylogenetic analysis based on the 16S rRNA gene sequence of strain CCB-QB4T showed 94.07, 92.69, 91.52 and 90.90 % sequence similarity to Algibacillus agarilyticus RQJ05T, Catenovulum maritimum Q1T, Catenovulum agarivorans YM01T and Catenovulum sediminis D2T, respectively.
34752210	1	3	dep	bacterium	72:80	arg1	nov.					39:42	nov.	39:42	nov.	39:42	nov., a novel alginate-degrading bacterium isolated from the surface of intertidal algal turf.
34752210	10	4	theme	polar	1095:1099	arg1	phosphatidylethanolamine					1113:1136	phosphatidylethanolamine	1113:1136	phosphatidylethanolamine	1113:1136	The polar lipids were phosphatidylethanolamine, two unidentified aminolipids, two unidentified glycolipids, an unidentified phospholipid and unidentified lipid.
34752210	10	4	theme	polar	1095:1099	arg1	lipids					1101:1106	The polar lipids	1091:1106	The polar lipids	1091:1106	The polar lipids were phosphatidylethanolamine, two unidentified aminolipids, two unidentified glycolipids, an unidentified phospholipid and unidentified lipid.
34752210	9	5	dep	feature	1024:1030	arg1	C18 					1078:1081	C18 	1078:1081	C18 	1078:1081	The major fatty acids were summed feature 3 (C16 : 1 ω7c/iso-C15 : 0 2-OH), C16 : 0 and C18 : 1 ω7c.
34752210	9	5	dep	feature	1024:1030	arg1	C16 					1035:1038	C16 	1035:1038	C16 	1035:1038	The major fatty acids were summed feature 3 (C16 : 1 ω7c/iso-C15 : 0 2-OH), C16 : 0 and C18 : 1 ω7c.
34752210	9	5	dep	feature	1024:1030	arg1	C16 					1066:1069	C16 	1066:1069	C16 	1066:1069	The major fatty acids were summed feature 3 (C16 : 1 ω7c/iso-C15 : 0 2-OH), C16 : 0 and C18 : 1 ω7c.
34752210	9	5	dep	feature	1024:1030	arg1	3					1032:1032	3	1032:1032	3	1032:1032	The major fatty acids were summed feature 3 (C16 : 1 ω7c/iso-C15 : 0 2-OH), C16 : 0 and C18 : 1 ω7c.
34752210	15	6	theme	33513T=CCB-MBL	1575:1588	arg1	CCB-QB4T					1560:1567	CCB-QB4T	1560:1567	CCB-QB4T (=JCM 33513T=CCB-MBL 5008T)	1560:1595	The type strain is CCB-QB4T (=JCM 33513T=CCB-MBL 5008T).
34752210	15	6	theme	33513T=CCB-MBL	1575:1588	arg1	5008T					1590:1594	=JCM 33513T=CCB-MBL 5008T	1570:1594	=JCM 33513T=CCB-MBL 5008T	1570:1594	The type strain is CCB-QB4T (=JCM 33513T=CCB-MBL 5008T).
34752210	2	7	theme	Penang	329:334	arg1	Malaysia					337:344	Penang, Malaysia	329:344	Malaysia	337:344	A novel rod-shaped, Gram-stain-negative, strictly aerobic and alginate-degrading marine bacterium, designated CCB-QB4T, was isolated from a surface of algal turf collected from a coastal area of Penang, Malaysia.
34752210	9	8	theme	major	994:998	arg1	acids					1006:1010	The major fatty acids	990:1010	The major fatty acids	990:1010	The major fatty acids were summed feature 3 (C16 : 1 ω7c/iso-C15 : 0 2-OH), C16 : 0 and C18 : 1 ω7c.
34752210	2	9	dep	rod-shaped	142:151	arg1	Gram-stain-negative					154:172	Gram-stain-negative	154:172	Gram-stain-negative	154:172	A novel rod-shaped, Gram-stain-negative, strictly aerobic and alginate-degrading marine bacterium, designated CCB-QB4T, was isolated from a surface of algal turf collected from a coastal area of Penang, Malaysia.
34752210	2	9	dep	rod-shaped	142:151	arg1	aerobic					184:190	aerobic	184:190	aerobic	184:190	A novel rod-shaped, Gram-stain-negative, strictly aerobic and alginate-degrading marine bacterium, designated CCB-QB4T, was isolated from a surface of algal turf collected from a coastal area of Penang, Malaysia.
34752210	2	9	dep	rod-shaped	142:151	arg1	alginate-degrading					196:213	alginate-degrading	196:213	alginate-degrading	196:213	A novel rod-shaped, Gram-stain-negative, strictly aerobic and alginate-degrading marine bacterium, designated CCB-QB4T, was isolated from a surface of algal turf collected from a coastal area of Penang, Malaysia.
34752210	8	10	theme	1-6 	960:963	arg1	%					964:964	%	964:964	%	964:964	Cell growth was observed at 15-40 °C, at pH 7.0-10.0 and in the presence of 1-6 % (w/v) NaCl and glucose.
34752210	8	11	theme	glucose	981:987	arg1	presence					948:955	the presence	944:955	the presence of 1-6 % (w/v) NaCl and glucose	944:987	Cell growth was observed at 15-40 °C, at pH 7.0-10.0 and in the presence of 1-6 % (w/v) NaCl and glucose.
34752210	12	12	theme	G+C	1316:1318	arg1	content					1320:1326	The genomic DNA G+C content	1300:1326	The genomic DNA G+C content	1300:1326	The genomic DNA G+C content was 46.7 mol%.
34752210	12	12	theme	G+C	1316:1318	arg1	%					1340:1340	46.7 mol%	1332:1340	46.7 mol%	1332:1340	The genomic DNA G+C content was 46.7 mol%.
34752210	9	13	theme	fatty	1000:1004	arg1	acids					1006:1010	The major fatty acids	990:1010	The major fatty acids	990:1010	The major fatty acids were summed feature 3 (C16 : 1 ω7c/iso-C15 : 0 2-OH), C16 : 0 and C18 : 1 ω7c.
34752210	13	14	theme	litoralis	1498:1506	arg1	nov.					1513:1516	the name Saccharobesus litoralis gen. nov.	1475:1516	the name Saccharobesus litoralis gen. nov.	1475:1516	Based on the phenotypic, chemotaxonomic and phylogenetic data, strain CCB-BQ4T represents a novel species in a new genus, for which the name Saccharobesus litoralis gen. nov., sp.
34752210	6	15	theme	Strain	724:729	arg1	CCB-QB4T					731:738	Strain CCB-QB4T	724:738	Strain CCB-QB4T	724:738	Strain CCB-QB4T formed a cluster with A. agarilyticus RQJ05T.
34752210	2	16	theme	novel	136:140	arg1	bacterium					222:230	A novel rod-shaped, Gram-stain-negative, strictly aerobic and alginate-degrading marine bacterium	134:230	A novel rod-shaped, Gram-stain-negative, strictly aerobic and alginate-degrading marine bacterium	134:230	A novel rod-shaped, Gram-stain-negative, strictly aerobic and alginate-degrading marine bacterium, designated CCB-QB4T, was isolated from a surface of algal turf collected from a coastal area of Penang, Malaysia.
34752210	2	17	attach	isolated	258:265	arg1	surface					274:280	a surface	272:280	a surface of algal turf collected from a coastal area of Penang, Malaysia	272:344	A novel rod-shaped, Gram-stain-negative, strictly aerobic and alginate-degrading marine bacterium, designated CCB-QB4T, was isolated from a surface of algal turf collected from a coastal area of Penang, Malaysia.
34752210	2	17	attach	isolated	258:265	arg2	bacterium					222:230	A novel rod-shaped, Gram-stain-negative, strictly aerobic and alginate-degrading marine bacterium	134:230	A novel rod-shaped, Gram-stain-negative, strictly aerobic and alginate-degrading marine bacterium	134:230	A novel rod-shaped, Gram-stain-negative, strictly aerobic and alginate-degrading marine bacterium, designated CCB-QB4T, was isolated from a surface of algal turf collected from a coastal area of Penang, Malaysia.
34752210	5	18	theme	sequence	565:572	arg1	similarity					574:583	94.07, 92.69, 91.52 and 90.90 % sequence similarity	533:583	94.07, 92.69, 91.52 and 90.90 % sequence similarity to Algibacillus agarilyticus RQJ05T, Catenovulum maritimum Q1T, Catenovulum agarivorans YM01T and Catenovulum sediminis D2T, respectively	533:721	Phylogenetic analysis based on the 16S rRNA gene sequence of strain CCB-QB4T showed 94.07, 92.69, 91.52 and 90.90 % sequence similarity to Algibacillus agarilyticus RQJ05T, Catenovulum maritimum Q1T, Catenovulum agarivorans YM01T and Catenovulum sediminis D2T, respectively.
34752210	12	19	theme	DNA	1312:1314	arg1	content					1320:1326	The genomic DNA G+C content	1300:1326	The genomic DNA G+C content	1300:1326	The genomic DNA G+C content was 46.7 mol%.
34752210	12	19	theme	DNA	1312:1314	arg1	%					1340:1340	46.7 mol%	1332:1340	46.7 mol%	1332:1340	The genomic DNA G+C content was 46.7 mol%.
34752210	12	20	theme	46.7 mol	1332:1339	arg1	content					1320:1326	The genomic DNA G+C content	1300:1326	The genomic DNA G+C content	1300:1326	The genomic DNA G+C content was 46.7 mol%.
34752210	12	20	theme	46.7 mol	1332:1339	arg1	%					1340:1340	46.7 mol%	1332:1340	46.7 mol%	1332:1340	The genomic DNA G+C content was 46.7 mol%.
34752210	15	21	theme	=JCM	1570:1573	arg1	CCB-QB4T					1560:1567	CCB-QB4T	1560:1567	CCB-QB4T (=JCM 33513T=CCB-MBL 5008T)	1560:1595	The type strain is CCB-QB4T (=JCM 33513T=CCB-MBL 5008T).
34752210	15	21	theme	=JCM	1570:1573	arg1	5008T					1590:1594	=JCM 33513T=CCB-MBL 5008T	1570:1594	=JCM 33513T=CCB-MBL 5008T	1570:1594	The type strain is CCB-QB4T (=JCM 33513T=CCB-MBL 5008T).
34752210	1	22	theme	novel	47:51	arg1	bacterium					72:80	a novel alginate-degrading bacterium	45:80	a novel alginate-degrading bacterium isolated from the surface of intertidal algal turf	45:131	nov., a novel alginate-degrading bacterium isolated from the surface of intertidal algal turf.
34752210	9	23	dep	C16 	1035:1038	arg1	 1 ω7c/iso-C15 					1040:1054	 1 ω7c/iso-C15 	1040:1054	 1 ω7c/iso-C15 	1040:1054	The major fatty acids were summed feature 3 (C16 : 1 ω7c/iso-C15 : 0 2-OH), C16 : 0 and C18 : 1 ω7c.
34752210	9	23	dep	C16 	1035:1038	arg1	2-OH					1059:1062	 0 2-OH	1056:1062	 0 2-OH	1056:1062	The major fatty acids were summed feature 3 (C16 : 1 ω7c/iso-C15 : 0 2-OH), C16 : 0 and C18 : 1 ω7c.
34752210	13	24	theme	novel	1435:1439	arg1	species					1441:1447	a novel species	1433:1447	a novel species	1433:1447	Based on the phenotypic, chemotaxonomic and phylogenetic data, strain CCB-BQ4T represents a novel species in a new genus, for which the name Saccharobesus litoralis gen. nov., sp.
34752210	5	25	dep	agarilyticus	601:612	arg1	Catenovulum					622:632	Catenovulum	622:632	Catenovulum	622:632	Phylogenetic analysis based on the 16S rRNA gene sequence of strain CCB-QB4T showed 94.07, 92.69, 91.52 and 90.90 % sequence similarity to Algibacillus agarilyticus RQJ05T, Catenovulum maritimum Q1T, Catenovulum agarivorans YM01T and Catenovulum sediminis D2T, respectively.
34752210	5	25	dep	agarilyticus	601:612	arg1	YM01T					673:677	YM01T	673:677	YM01T	673:677	Phylogenetic analysis based on the 16S rRNA gene sequence of strain CCB-QB4T showed 94.07, 92.69, 91.52 and 90.90 % sequence similarity to Algibacillus agarilyticus RQJ05T, Catenovulum maritimum Q1T, Catenovulum agarivorans YM01T and Catenovulum sediminis D2T, respectively.
34752210	5	25	dep	agarilyticus	601:612	arg1	Catenovulum					649:659	Catenovulum	649:659	Catenovulum	649:659	Phylogenetic analysis based on the 16S rRNA gene sequence of strain CCB-QB4T showed 94.07, 92.69, 91.52 and 90.90 % sequence similarity to Algibacillus agarilyticus RQJ05T, Catenovulum maritimum Q1T, Catenovulum agarivorans YM01T and Catenovulum sediminis D2T, respectively.
34752210	5	25	dep	agarilyticus	601:612	arg1	D2T					705:707	D2T	705:707	D2T	705:707	Phylogenetic analysis based on the 16S rRNA gene sequence of strain CCB-QB4T showed 94.07, 92.69, 91.52 and 90.90 % sequence similarity to Algibacillus agarilyticus RQJ05T, Catenovulum maritimum Q1T, Catenovulum agarivorans YM01T and Catenovulum sediminis D2T, respectively.
34752210	8	26	theme	%	964:964	arg1	NaCl					972:975	NaCl	972:975	NaCl	972:975	Cell growth was observed at 15-40 °C, at pH 7.0-10.0 and in the presence of 1-6 % (w/v) NaCl and glucose.
34752210	1	27	theme	alginate-degrading	53:70	arg1	bacterium					72:80	a novel alginate-degrading bacterium	45:80	a novel alginate-degrading bacterium isolated from the surface of intertidal algal turf	45:131	nov., a novel alginate-degrading bacterium isolated from the surface of intertidal algal turf.
34752210	5	28	theme	16S	484:486	arg1	sequence					498:505	the 16S rRNA gene sequence	480:505	the 16S rRNA gene sequence of strain CCB-QB4T	480:524	Phylogenetic analysis based on the 16S rRNA gene sequence of strain CCB-QB4T showed 94.07, 92.69, 91.52 and 90.90 % sequence similarity to Algibacillus agarilyticus RQJ05T, Catenovulum maritimum Q1T, Catenovulum agarivorans YM01T and Catenovulum sediminis D2T, respectively.
34752210	13	29	theme	strain	1406:1411	arg1	CCB-BQ4T					1413:1420	strain CCB-BQ4T	1406:1420	strain CCB-BQ4T	1406:1420	Based on the phenotypic, chemotaxonomic and phylogenetic data, strain CCB-BQ4T represents a novel species in a new genus, for which the name Saccharobesus litoralis gen. nov., sp.
34752210	0	30	theme	litoralis	14:22	arg1	nov.					29:32	Saccharobesus litoralis gen. nov.	0:32	Saccharobesus litoralis gen. nov.	0:32	Saccharobesus litoralis gen. nov., sp.
34752210	6	31	theme	agarilyticus	765:776	arg1	RQJ05T					778:783	A. agarilyticus RQJ05T	762:783	A. agarilyticus RQJ05T	762:783	Strain CCB-QB4T formed a cluster with A. agarilyticus RQJ05T.
34752210	5	32	theme	rRNA	488:491	arg1	sequence					498:505	the 16S rRNA gene sequence	480:505	the 16S rRNA gene sequence of strain CCB-QB4T	480:524	Phylogenetic analysis based on the 16S rRNA gene sequence of strain CCB-QB4T showed 94.07, 92.69, 91.52 and 90.90 % sequence similarity to Algibacillus agarilyticus RQJ05T, Catenovulum maritimum Q1T, Catenovulum agarivorans YM01T and Catenovulum sediminis D2T, respectively.
34752210	0	33	theme	Saccharobesus	0:12	arg1	nov.					29:32	Saccharobesus litoralis gen. nov.	0:32	Saccharobesus litoralis gen. nov.	0:32	Saccharobesus litoralis gen. nov., sp.
34752210	8	34	located	observed	900:907	arg1	15-40 °C					912:919	15-40 °C	912:919	15-40 °C	912:919	Cell growth was observed at 15-40 °C, at pH 7.0-10.0 and in the presence of 1-6 % (w/v) NaCl and glucose.
34752210	8	34	located	observed	900:907	arg2	growth					889:894	Cell growth	884:894	Cell growth	884:894	Cell growth was observed at 15-40 °C, at pH 7.0-10.0 and in the presence of 1-6 % (w/v) NaCl and glucose.
34752210	6	35	theme	A.	762:763	arg1	RQJ05T					778:783	A. agarilyticus RQJ05T	762:783	A. agarilyticus RQJ05T	762:783	Strain CCB-QB4T formed a cluster with A. agarilyticus RQJ05T.
34752210	8	36	theme	Cell	884:887	arg1	growth					889:894	Cell growth	884:894	Cell growth	884:894	Cell growth was observed at 15-40 °C, at pH 7.0-10.0 and in the presence of 1-6 % (w/v) NaCl and glucose.
34752210	15	37	theme	type	1545:1548	arg1	CCB-QB4T					1560:1567	CCB-QB4T	1560:1567	CCB-QB4T (=JCM 33513T=CCB-MBL 5008T)	1560:1595	The type strain is CCB-QB4T (=JCM 33513T=CCB-MBL 5008T).
34752210	15	37	theme	type	1545:1548	arg1	strain					1550:1555	The type strain	1541:1555	The type strain	1541:1555	The type strain is CCB-QB4T (=JCM 33513T=CCB-MBL 5008T).
34752210	13	38	dep	sp	1519:1520	arg1	nov.					1513:1516	the name Saccharobesus litoralis gen. nov.	1475:1516	the name Saccharobesus litoralis gen. nov.	1475:1516	Based on the phenotypic, chemotaxonomic and phylogenetic data, strain CCB-BQ4T represents a novel species in a new genus, for which the name Saccharobesus litoralis gen. nov., sp.
34752210	2	39	theme	turf	291:294	arg1	surface					274:280	a surface	272:280	a surface of algal turf collected from a coastal area of Penang, Malaysia	272:344	A novel rod-shaped, Gram-stain-negative, strictly aerobic and alginate-degrading marine bacterium, designated CCB-QB4T, was isolated from a surface of algal turf collected from a coastal area of Penang, Malaysia.
34752210	5	40	theme	Phylogenetic	449:460	arg1	analysis					462:469	Phylogenetic analysis	449:469	Phylogenetic analysis based on the 16S rRNA gene sequence of strain CCB-QB4T	449:524	Phylogenetic analysis based on the 16S rRNA gene sequence of strain CCB-QB4T showed 94.07, 92.69, 91.52 and 90.90 % sequence similarity to Algibacillus agarilyticus RQJ05T, Catenovulum maritimum Q1T, Catenovulum agarivorans YM01T and Catenovulum sediminis D2T, respectively.
34752210	13	41	theme	chemotaxonomic	1368:1381	arg1	data					1400:1403	the phenotypic, chemotaxonomic and phylogenetic data	1352:1403	the phenotypic, chemotaxonomic and phylogenetic data	1352:1403	Based on the phenotypic, chemotaxonomic and phylogenetic data, strain CCB-BQ4T represents a novel species in a new genus, for which the name Saccharobesus litoralis gen. nov., sp.
34752210	0	42	theme	gen.	24:27	arg1	nov.					29:32	Saccharobesus litoralis gen. nov.	0:32	Saccharobesus litoralis gen. nov.	0:32	Saccharobesus litoralis gen. nov., sp.
34752210	13	43	theme	gen.	1508:1511	arg1	nov.					1513:1516	the name Saccharobesus litoralis gen. nov.	1475:1516	the name Saccharobesus litoralis gen. nov.	1475:1516	Based on the phenotypic, chemotaxonomic and phylogenetic data, strain CCB-BQ4T represents a novel species in a new genus, for which the name Saccharobesus litoralis gen. nov., sp.
34752210	4	44	theme	long	425:428	arg1	chains					430:435	long chains	425:435	long chains end-to-end	425:446	The rod-shaped cells formed long chains end-to-end.
34752210	2	45	theme	algal	285:289	arg1	turf					291:294	algal turf	285:294	algal turf collected from a coastal area of Penang, Malaysia	285:344	A novel rod-shaped, Gram-stain-negative, strictly aerobic and alginate-degrading marine bacterium, designated CCB-QB4T, was isolated from a surface of algal turf collected from a coastal area of Penang, Malaysia.
34752210	10	46	theme	unidentified	1202:1213	arg1	phosphatidylethanolamine					1113:1136	phosphatidylethanolamine	1113:1136	phosphatidylethanolamine	1113:1136	The polar lipids were phosphatidylethanolamine, two unidentified aminolipids, two unidentified glycolipids, an unidentified phospholipid and unidentified lipid.
34752210	10	46	theme	unidentified	1202:1213	arg1	phospholipid					1215:1226	an unidentified phospholipid	1199:1226	an unidentified phospholipid	1199:1226	The polar lipids were phosphatidylethanolamine, two unidentified aminolipids, two unidentified glycolipids, an unidentified phospholipid and unidentified lipid.
34752210	3	47	theme	lateral	378:384	arg1	flagellum					386:394	a lateral flagellum	376:394	a lateral flagellum	376:394	The cells showed motility by a lateral flagellum.
34752210	7	48	theme	Strain	786:791	arg1	CCB-QB4T					793:800	Strain CCB-QB4T	786:800	Strain CCB-QB4T	786:800	Strain CCB-QB4T was catalase-negative, oxidase-positive, and degraded agar, alginate, and starch.
34752210	13	49	theme	name	1479:1482	arg1	nov.					1513:1516	the name Saccharobesus litoralis gen. nov.	1475:1516	the name Saccharobesus litoralis gen. nov.	1475:1516	Based on the phenotypic, chemotaxonomic and phylogenetic data, strain CCB-BQ4T represents a novel species in a new genus, for which the name Saccharobesus litoralis gen. nov., sp.
34752210	10	50	theme	unidentified	1143:1154	arg1	phosphatidylethanolamine					1113:1136	phosphatidylethanolamine	1113:1136	phosphatidylethanolamine	1113:1136	The polar lipids were phosphatidylethanolamine, two unidentified aminolipids, two unidentified glycolipids, an unidentified phospholipid and unidentified lipid.
34752210	10	50	theme	unidentified	1143:1154	arg1	aminolipids					1156:1166	two unidentified aminolipids	1139:1166	two unidentified aminolipids	1139:1166	The polar lipids were phosphatidylethanolamine, two unidentified aminolipids, two unidentified glycolipids, an unidentified phospholipid and unidentified lipid.
34752210	13	51	theme	phylogenetic	1387:1398	arg1	data					1400:1403	the phenotypic, chemotaxonomic and phylogenetic data	1352:1403	the phenotypic, chemotaxonomic and phylogenetic data	1352:1403	Based on the phenotypic, chemotaxonomic and phylogenetic data, strain CCB-BQ4T represents a novel species in a new genus, for which the name Saccharobesus litoralis gen. nov., sp.
34752210	1	52	attach	isolated	82:89	arg1	surface					100:106	the surface	96:106	the surface of intertidal algal turf	96:131	nov., a novel alginate-degrading bacterium isolated from the surface of intertidal algal turf.
34752210	1	52	attach	isolated	82:89	arg2	bacterium					72:80	a novel alginate-degrading bacterium	45:80	a novel alginate-degrading bacterium isolated from the surface of intertidal algal turf	45:131	nov., a novel alginate-degrading bacterium isolated from the surface of intertidal algal turf.
34752210	12	53	theme	genomic	1304:1310	arg1	content					1320:1326	The genomic DNA G+C content	1300:1326	The genomic DNA G+C content	1300:1326	The genomic DNA G+C content was 46.7 mol%.
34752210	12	53	theme	genomic	1304:1310	arg1	%					1340:1340	46.7 mol%	1332:1340	46.7 mol%	1332:1340	The genomic DNA G+C content was 46.7 mol%.
34752210	4	54	theme	end-to-end	437:446	arg1	chains					430:435	long chains	425:435	long chains end-to-end	425:446	The rod-shaped cells formed long chains end-to-end.
34752210	13	55	theme	new	1454:1456	arg1	genus					1458:1462	a new genus	1452:1462	a new genus	1452:1462	Based on the phenotypic, chemotaxonomic and phylogenetic data, strain CCB-BQ4T represents a novel species in a new genus, for which the name Saccharobesus litoralis gen. nov., sp.
34752210	5	56	theme	CCB-QB4T	517:524	arg1	sequence					498:505	the 16S rRNA gene sequence	480:505	the 16S rRNA gene sequence of strain CCB-QB4T	480:524	Phylogenetic analysis based on the 16S rRNA gene sequence of strain CCB-QB4T showed 94.07, 92.69, 91.52 and 90.90 % sequence similarity to Algibacillus agarilyticus RQJ05T, Catenovulum maritimum Q1T, Catenovulum agarivorans YM01T and Catenovulum sediminis D2T, respectively.
34752210	10	57	theme	unidentified	1173:1184	arg1	glycolipids					1186:1196	two unidentified glycolipids	1169:1196	two unidentified glycolipids	1169:1196	The polar lipids were phosphatidylethanolamine, two unidentified aminolipids, two unidentified glycolipids, an unidentified phospholipid and unidentified lipid.
34752210	10	57	theme	unidentified	1173:1184	arg1	phosphatidylethanolamine					1113:1136	phosphatidylethanolamine	1113:1136	phosphatidylethanolamine	1113:1136	The polar lipids were phosphatidylethanolamine, two unidentified aminolipids, two unidentified glycolipids, an unidentified phospholipid and unidentified lipid.
34752210	11	58	theme	major	1256:1260	arg1	ubiquinone-8					1286:1297	ubiquinone-8	1286:1297	ubiquinone-8	1286:1297	The major respiratory quinone was ubiquinone-8.
34752210	11	58	theme	major	1256:1260	arg1	quinone					1274:1280	The major respiratory quinone	1252:1280	The major respiratory quinone	1252:1280	The major respiratory quinone was ubiquinone-8.
34752210	2	59	theme	rod-shaped	142:151	arg1	bacterium					222:230	A novel rod-shaped, Gram-stain-negative, strictly aerobic and alginate-degrading marine bacterium	134:230	A novel rod-shaped, Gram-stain-negative, strictly aerobic and alginate-degrading marine bacterium	134:230	A novel rod-shaped, Gram-stain-negative, strictly aerobic and alginate-degrading marine bacterium, designated CCB-QB4T, was isolated from a surface of algal turf collected from a coastal area of Penang, Malaysia.
34752210	0	60	dep	sp	35:36	arg1	nov.					29:32	Saccharobesus litoralis gen. nov.	0:32	Saccharobesus litoralis gen. nov.	0:32	Saccharobesus litoralis gen. nov., sp.
34752210	8	61	dep	%	964:964	arg1	w/v					967:969	w/v	967:969	w/v	967:969	Cell growth was observed at 15-40 °C, at pH 7.0-10.0 and in the presence of 1-6 % (w/v) NaCl and glucose.
34752210	11	62	theme	respiratory	1262:1272	arg1	ubiquinone-8					1286:1297	ubiquinone-8	1286:1297	ubiquinone-8	1286:1297	The major respiratory quinone was ubiquinone-8.
34752210	11	62	theme	respiratory	1262:1272	arg1	quinone					1274:1280	The major respiratory quinone	1252:1280	The major respiratory quinone	1252:1280	The major respiratory quinone was ubiquinone-8.
34752210	13	63	theme	phenotypic	1356:1365	arg1	data					1400:1403	the phenotypic, chemotaxonomic and phylogenetic data	1352:1403	the phenotypic, chemotaxonomic and phylogenetic data	1352:1403	Based on the phenotypic, chemotaxonomic and phylogenetic data, strain CCB-BQ4T represents a novel species in a new genus, for which the name Saccharobesus litoralis gen. nov., sp.
34752210	2	64	theme	marine	215:220	arg1	bacterium					222:230	A novel rod-shaped, Gram-stain-negative, strictly aerobic and alginate-degrading marine bacterium	134:230	A novel rod-shaped, Gram-stain-negative, strictly aerobic and alginate-degrading marine bacterium	134:230	A novel rod-shaped, Gram-stain-negative, strictly aerobic and alginate-degrading marine bacterium, designated CCB-QB4T, was isolated from a surface of algal turf collected from a coastal area of Penang, Malaysia.
34752210	10	65	theme	unidentified	1232:1243	arg1	phosphatidylethanolamine					1113:1136	phosphatidylethanolamine	1113:1136	phosphatidylethanolamine	1113:1136	The polar lipids were phosphatidylethanolamine, two unidentified aminolipids, two unidentified glycolipids, an unidentified phospholipid and unidentified lipid.
34752210	10	65	theme	unidentified	1232:1243	arg1	lipid					1245:1249	unidentified lipid	1232:1249	unidentified lipid	1232:1249	The polar lipids were phosphatidylethanolamine, two unidentified aminolipids, two unidentified glycolipids, an unidentified phospholipid and unidentified lipid.
34752210	1	66	theme	intertidal	111:120	arg1	turf					128:131	intertidal algal turf	111:131	intertidal algal turf	111:131	nov., a novel alginate-degrading bacterium isolated from the surface of intertidal algal turf.
34752210	5	67	dep	similarity	574:583	arg1	%					563:563	%	563:563	%	563:563	Phylogenetic analysis based on the 16S rRNA gene sequence of strain CCB-QB4T showed 94.07, 92.69, 91.52 and 90.90 % sequence similarity to Algibacillus agarilyticus RQJ05T, Catenovulum maritimum Q1T, Catenovulum agarivorans YM01T and Catenovulum sediminis D2T, respectively.
34752210	13	68	theme	Saccharobesus	1484:1496	arg1	nov.					1513:1516	the name Saccharobesus litoralis gen. nov.	1475:1516	the name Saccharobesus litoralis gen. nov.	1475:1516	Based on the phenotypic, chemotaxonomic and phylogenetic data, strain CCB-BQ4T represents a novel species in a new genus, for which the name Saccharobesus litoralis gen. nov., sp.
34752210	5	69	theme	gene	493:496	arg1	sequence					498:505	the 16S rRNA gene sequence	480:505	the 16S rRNA gene sequence of strain CCB-QB4T	480:524	Phylogenetic analysis based on the 16S rRNA gene sequence of strain CCB-QB4T showed 94.07, 92.69, 91.52 and 90.90 % sequence similarity to Algibacillus agarilyticus RQJ05T, Catenovulum maritimum Q1T, Catenovulum agarivorans YM01T and Catenovulum sediminis D2T, respectively.
34752210	1	70	theme	algal	122:126	arg1	turf					128:131	intertidal algal turf	111:131	intertidal algal turf	111:131	nov., a novel alginate-degrading bacterium isolated from the surface of intertidal algal turf.
34752210	2	71	theme	coastal	313:319	arg1	area					321:324	a coastal area	311:324	a coastal area of Penang, Malaysia	311:344	A novel rod-shaped, Gram-stain-negative, strictly aerobic and alginate-degrading marine bacterium, designated CCB-QB4T, was isolated from a surface of algal turf collected from a coastal area of Penang, Malaysia.
34752210	4	72	theme	rod-shaped	401:410	arg1	cells					412:416	The rod-shaped cells	397:416	The rod-shaped cells	397:416	The rod-shaped cells formed long chains end-to-end.
34752210	1	73	theme	turf	128:131	arg1	surface					100:106	the surface	96:106	the surface of intertidal algal turf	96:131	nov., a novel alginate-degrading bacterium isolated from the surface of intertidal algal turf.
34752210	5	74	dep	%	563:563	arg1	91.52					547:551	91.52	547:551	91.52	547:551	Phylogenetic analysis based on the 16S rRNA gene sequence of strain CCB-QB4T showed 94.07, 92.69, 91.52 and 90.90 % sequence similarity to Algibacillus agarilyticus RQJ05T, Catenovulum maritimum Q1T, Catenovulum agarivorans YM01T and Catenovulum sediminis D2T, respectively.
34752210	5	74	dep	%	563:563	arg1	90.90 					557:562	90.90 	557:562	90.90 	557:562	Phylogenetic analysis based on the 16S rRNA gene sequence of strain CCB-QB4T showed 94.07, 92.69, 91.52 and 90.90 % sequence similarity to Algibacillus agarilyticus RQJ05T, Catenovulum maritimum Q1T, Catenovulum agarivorans YM01T and Catenovulum sediminis D2T, respectively.
34752210	2	75	theme	Malaysia	337:344	arg1	area					321:324	a coastal area	311:324	a coastal area of Penang, Malaysia	311:344	A novel rod-shaped, Gram-stain-negative, strictly aerobic and alginate-degrading marine bacterium, designated CCB-QB4T, was isolated from a surface of algal turf collected from a coastal area of Penang, Malaysia.
33466274	0	0	from	Effects	13:19	arg1	Microbiota					65:74	Intestinal Microbiota	54:74	Intestinal Microbiota	54:74	The Combined Effects of Magnesium Oxide and Inulin on Intestinal Microbiota and Cecal Short-Chain Fatty Acids.
33466274	0	0	from	Effects	13:19	arg1	Acids					104:108	Cecal Short-Chain Fatty Acids	80:108	Cecal Short-Chain Fatty Acids	80:108	The Combined Effects of Magnesium Oxide and Inulin on Intestinal Microbiota and Cecal Short-Chain Fatty Acids.
33466274	8	1	theme	SCFA	999:1002	arg1	concentrations					1004:1017	the cecal SCFA concentrations	989:1017	the cecal SCFA concentrations elevated by inulin	989:1036	Inulin intake decreased cecal pH and increased cecal SCFA concentrations while combining MgO increased the cecal pH lowered by inulin and decreased the cecal SCFA concentrations elevated by inulin.
33466274	6	2	theme	timing	676:681	arg1	effect					683:688	the MgO administration timing effect	653:688	the MgO administration timing effect on cecal SCFAs	653:703	We also examined the MgO administration timing effect on cecal SCFAs.
33466274	4	3	theme	physiological	453:465	arg1	functions					467:475	systemic physiological functions	444:475	systemic physiological functions	444:475	SCFAs are involved in regulating systemic physiological functions and circadian rhythm.
33466274	4	4	theme	circadian	481:489	arg1	rhythm					491:496	circadian rhythm	481:496	circadian rhythm	481:496	SCFAs are involved in regulating systemic physiological functions and circadian rhythm.
33466274	8	5	theme	cecal	993:997	arg1	concentrations					1004:1017	the cecal SCFA concentrations	989:1017	the cecal SCFA concentrations elevated by inulin	989:1036	Inulin intake decreased cecal pH and increased cecal SCFA concentrations while combining MgO increased the cecal pH lowered by inulin and decreased the cecal SCFA concentrations elevated by inulin.
33466274	5	6	theme	dietary	561:567	arg1	fiber					569:573	the water-soluble dietary fiber	543:573	the water-soluble dietary fiber	543:573	We examined the effect of combining MgO and the water-soluble dietary fiber, inulin, on cecal SCFA concentration and microbiota in mice.
33466274	5	6	theme	dietary	561:567	arg1	inulin					576:581	inulin	576:581	inulin	576:581	We examined the effect of combining MgO and the water-soluble dietary fiber, inulin, on cecal SCFA concentration and microbiota in mice.
33466274	3	7	theme	fatty	390:394	arg1	SCFAs					403:407	SCFAs	403:407	SCFAs	403:407	Dietary fiber is fermented by microbiota to produce short-chain fatty acids (SCFAs).
33466274	3	7	theme	fatty	390:394	arg1	acids					396:400	short-chain fatty acids	378:400	short-chain fatty acids (SCFAs)	378:408	Dietary fiber is fermented by microbiota to produce short-chain fatty acids (SCFAs).
33466274	0	8	theme	Short-Chain	86:96	arg1	Acids					104:108	Cecal Short-Chain Fatty Acids	80:108	Cecal Short-Chain Fatty Acids	80:108	The Combined Effects of Magnesium Oxide and Inulin on Intestinal Microbiota and Cecal Short-Chain Fatty Acids.
33466274	8	9	theme	elevated	1019:1026	arg1	concentrations					1004:1017	the cecal SCFA concentrations	989:1017	the cecal SCFA concentrations elevated by inulin	989:1036	Inulin intake decreased cecal pH and increased cecal SCFA concentrations while combining MgO increased the cecal pH lowered by inulin and decreased the cecal SCFA concentrations elevated by inulin.
33466274	8	10	theme	cecal	948:952	arg1	pH					954:955	the cecal pH	944:955	the cecal pH lowered by inulin	944:973	Inulin intake decreased cecal pH and increased cecal SCFA concentrations while combining MgO increased the cecal pH lowered by inulin and decreased the cecal SCFA concentrations elevated by inulin.
33466274	2	11	theme	chronic	253:259	arg1	constipation					261:272	chronic constipation	253:272	chronic constipation	253:272	While magnesium oxide (MgO) is often used as the first-line drug for chronic constipation in Japan, dietary fiber intake is also recommended.
33466274	0	12	theme	Cecal	80:84	arg1	Acids					104:108	Cecal Short-Chain Fatty Acids	80:108	Cecal Short-Chain Fatty Acids	80:108	The Combined Effects of Magnesium Oxide and Inulin on Intestinal Microbiota and Cecal Short-Chain Fatty Acids.
33466274	9	13	theme	significant	1074:1084	arg1	changes					1086:1092	significant changes	1074:1092	significant changes in the microbiota composition	1074:1122	When inulin and MgO were combined, significant changes in the microbiota composition were observed compared with inulin alone.
33466274	6	14	theme	administration	661:674	arg1	effect					683:688	the MgO administration timing effect	653:688	the MgO administration timing effect on cecal SCFAs	653:703	We also examined the MgO administration timing effect on cecal SCFAs.
33466274	10	15	theme	MgO	1170:1172	arg1	effect					1174:1179	The MgO effect	1166:1179	The MgO effect on the cecal acetic acid concentration	1166:1218	The MgO effect on the cecal acetic acid concentration was less when administered at ZT12 than at ZT0.
33466274	10	15	theme	MgO	1170:1172	arg1	less					1224:1227	less	1224:1227	less	1224:1227	The MgO effect on the cecal acetic acid concentration was less when administered at ZT12 than at ZT0.
33466274	11	16	theme	inulin	1353:1358	arg1	feeding					1360:1366	inulin feeding	1353:1366	inulin feeding	1353:1366	In conclusion, this study suggests that MgO affects cecal SCFA and microbiota during inulin feeding, and the effect on acetic acid concentration is time-dependent.
33466274	7	17	theme	next-generation	813:827	arg1	sequencing					829:838	next-generation sequencing	813:838	next-generation sequencing	813:838	The cecal SCFA concentrations were measured by gas chromatography, and the microbiota was determined using next-generation sequencing.
33466274	2	18	used	used	221:224	arg2	oxide					200:204	magnesium oxide	190:204	magnesium oxide (MgO)	190:210	While magnesium oxide (MgO) is often used as the first-line drug for chronic constipation in Japan, dietary fiber intake is also recommended.
33466274	2	18	used	used	221:224	arg2	drug					244:247	the first-line drug	229:247	the first-line drug for chronic constipation in Japan	229:281	While magnesium oxide (MgO) is often used as the first-line drug for chronic constipation in Japan, dietary fiber intake is also recommended.
33466274	2	18	used	used	221:224	arg2	MgO					207:209	MgO	207:209	MgO	207:209	While magnesium oxide (MgO) is often used as the first-line drug for chronic constipation in Japan, dietary fiber intake is also recommended.
33466274	11	19	theme	acid	1394:1397	arg1	concentration					1399:1411	acetic acid concentration	1387:1411	acetic acid concentration	1387:1411	In conclusion, this study suggests that MgO affects cecal SCFA and microbiota during inulin feeding, and the effect on acetic acid concentration is time-dependent.
33466274	0	20	theme	Fatty	98:102	arg1	Acids					104:108	Cecal Short-Chain Fatty Acids	80:108	Cecal Short-Chain Fatty Acids	80:108	The Combined Effects of Magnesium Oxide and Inulin on Intestinal Microbiota and Cecal Short-Chain Fatty Acids.
33466274	4	21	theme	systemic	444:451	arg1	functions					467:475	systemic physiological functions	444:475	systemic physiological functions	444:475	SCFAs are involved in regulating systemic physiological functions and circadian rhythm.
33466274	2	22	theme	first-line	233:242	arg1	drug					244:247	the first-line drug	229:247	the first-line drug for chronic constipation in Japan	229:281	While magnesium oxide (MgO) is often used as the first-line drug for chronic constipation in Japan, dietary fiber intake is also recommended.
33466274	2	22	theme	first-line	233:242	arg1	oxide					200:204	magnesium oxide	190:204	magnesium oxide (MgO)	190:210	While magnesium oxide (MgO) is often used as the first-line drug for chronic constipation in Japan, dietary fiber intake is also recommended.
33466274	1	23	theme	many	161:164	arg1	worldwide					173:181	many people worldwide	161:181	many people worldwide	161:181	Constipation is a common condition that occurs in many people worldwide.
33466274	0	24	theme	Combined	4:11	arg1	Effects					13:19	The Combined Effects	0:19	The Combined Effects of Magnesium Oxide and Inulin on Intestinal Microbiota and Cecal Short-Chain Fatty Acids	0:108	The Combined Effects of Magnesium Oxide and Inulin on Intestinal Microbiota and Cecal Short-Chain Fatty Acids.
33466274	7	25	theme	gas	753:755	arg1	chromatography					757:770	gas chromatography	753:770	gas chromatography	753:770	The cecal SCFA concentrations were measured by gas chromatography, and the microbiota was determined using next-generation sequencing.
33466274	5	26	theme	cecal	587:591	arg1	concentration					598:610	cecal SCFA concentration	587:610	cecal SCFA concentration	587:610	We examined the effect of combining MgO and the water-soluble dietary fiber, inulin, on cecal SCFA concentration and microbiota in mice.
33466274	8	27	theme	Inulin	841:846	arg1	intake					848:853	Inulin intake	841:853	Inulin intake	841:853	Inulin intake decreased cecal pH and increased cecal SCFA concentrations while combining MgO increased the cecal pH lowered by inulin and decreased the cecal SCFA concentrations elevated by inulin.
33466274	1	28	theme	people	166:171	arg1	worldwide					173:181	many people worldwide	161:181	many people worldwide	161:181	Constipation is a common condition that occurs in many people worldwide.
33466274	5	29	theme	SCFA	593:596	arg1	concentration					598:610	cecal SCFA concentration	587:610	cecal SCFA concentration	587:610	We examined the effect of combining MgO and the water-soluble dietary fiber, inulin, on cecal SCFA concentration and microbiota in mice.
33466274	5	30	theme	water-soluble	547:559	arg1	fiber					569:573	the water-soluble dietary fiber	543:573	the water-soluble dietary fiber	543:573	We examined the effect of combining MgO and the water-soluble dietary fiber, inulin, on cecal SCFA concentration and microbiota in mice.
33466274	5	30	theme	water-soluble	547:559	arg1	inulin					576:581	inulin	576:581	inulin	576:581	We examined the effect of combining MgO and the water-soluble dietary fiber, inulin, on cecal SCFA concentration and microbiota in mice.
33466274	8	31	theme	cecal	865:869	arg1	pH					871:872	cecal pH	865:872	cecal pH	865:872	Inulin intake decreased cecal pH and increased cecal SCFA concentrations while combining MgO increased the cecal pH lowered by inulin and decreased the cecal SCFA concentrations elevated by inulin.
33466274	8	32	theme	increased	878:886	arg1	concentrations					899:912	increased cecal SCFA concentrations	878:912	increased cecal SCFA concentrations	878:912	Inulin intake decreased cecal pH and increased cecal SCFA concentrations while combining MgO increased the cecal pH lowered by inulin and decreased the cecal SCFA concentrations elevated by inulin.
33466274	6	33	from	effect	683:688	arg1	SCFAs					699:703	cecal SCFAs	693:703	cecal SCFAs	693:703	We also examined the MgO administration timing effect on cecal SCFAs.
33466274	0	34	theme	Oxide	34:38	arg1	Effects					13:19	The Combined Effects	0:19	The Combined Effects of Magnesium Oxide and Inulin on Intestinal Microbiota and Cecal Short-Chain Fatty Acids	0:108	The Combined Effects of Magnesium Oxide and Inulin on Intestinal Microbiota and Cecal Short-Chain Fatty Acids.
33466274	6	35	theme	MgO	657:659	arg1	effect					683:688	the MgO administration timing effect	653:688	the MgO administration timing effect on cecal SCFAs	653:703	We also examined the MgO administration timing effect on cecal SCFAs.
33466274	7	36	theme	SCFA	716:719	arg1	concentrations					721:734	The cecal SCFA concentrations	706:734	The cecal SCFA concentrations	706:734	The cecal SCFA concentrations were measured by gas chromatography, and the microbiota was determined using next-generation sequencing.
33466274	11	37	theme	acetic	1387:1392	arg1	concentration					1399:1411	acetic acid concentration	1387:1411	acetic acid concentration	1387:1411	In conclusion, this study suggests that MgO affects cecal SCFA and microbiota during inulin feeding, and the effect on acetic acid concentration is time-dependent.
33466274	0	38	theme	Magnesium	24:32	arg1	Oxide					34:38	Magnesium Oxide	24:38	Magnesium Oxide	24:38	The Combined Effects of Magnesium Oxide and Inulin on Intestinal Microbiota and Cecal Short-Chain Fatty Acids.
33466274	5	39	from	microbiota	616:625	arg1	mice					630:633	mice	630:633	mice	630:633	We examined the effect of combining MgO and the water-soluble dietary fiber, inulin, on cecal SCFA concentration and microbiota in mice.
33466274	3	40	theme	short-chain	378:388	arg1	SCFAs					403:407	SCFAs	403:407	SCFAs	403:407	Dietary fiber is fermented by microbiota to produce short-chain fatty acids (SCFAs).
33466274	3	40	theme	short-chain	378:388	arg1	acids					396:400	short-chain fatty acids	378:400	short-chain fatty acids (SCFAs)	378:408	Dietary fiber is fermented by microbiota to produce short-chain fatty acids (SCFAs).
33466274	9	41	from	changes	1086:1092	arg1	composition					1112:1122	the microbiota composition	1097:1122	the microbiota composition	1097:1122	When inulin and MgO were combined, significant changes in the microbiota composition were observed compared with inulin alone.
33466274	8	42	theme	SCFA	894:897	arg1	concentrations					899:912	increased cecal SCFA concentrations	878:912	increased cecal SCFA concentrations	878:912	Inulin intake decreased cecal pH and increased cecal SCFA concentrations while combining MgO increased the cecal pH lowered by inulin and decreased the cecal SCFA concentrations elevated by inulin.
33466274	5	43	from	effect	515:520	arg1	microbiota					616:625	microbiota	616:625	microbiota	616:625	We examined the effect of combining MgO and the water-soluble dietary fiber, inulin, on cecal SCFA concentration and microbiota in mice.
33466274	5	43	from	effect	515:520	arg1	concentration					598:610	cecal SCFA concentration	587:610	cecal SCFA concentration	587:610	We examined the effect of combining MgO and the water-soluble dietary fiber, inulin, on cecal SCFA concentration and microbiota in mice.
33466274	0	44	theme	Inulin	44:49	arg1	Effects					13:19	The Combined Effects	0:19	The Combined Effects of Magnesium Oxide and Inulin on Intestinal Microbiota and Cecal Short-Chain Fatty Acids	0:108	The Combined Effects of Magnesium Oxide and Inulin on Intestinal Microbiota and Cecal Short-Chain Fatty Acids.
33466274	10	45	from	effect	1174:1179	arg1	concentration					1206:1218	the cecal acetic acid concentration	1184:1218	the cecal acetic acid concentration	1184:1218	The MgO effect on the cecal acetic acid concentration was less when administered at ZT12 than at ZT0.
33466274	8	46	theme	cecal	888:892	arg1	concentrations					899:912	increased cecal SCFA concentrations	878:912	increased cecal SCFA concentrations	878:912	Inulin intake decreased cecal pH and increased cecal SCFA concentrations while combining MgO increased the cecal pH lowered by inulin and decreased the cecal SCFA concentrations elevated by inulin.
33466274	5	47	from	concentration	598:610	arg1	mice					630:633	mice	630:633	mice	630:633	We examined the effect of combining MgO and the water-soluble dietary fiber, inulin, on cecal SCFA concentration and microbiota in mice.
33466274	9	48	theme	microbiota	1101:1110	arg1	composition					1112:1122	the microbiota composition	1097:1122	the microbiota composition	1097:1122	When inulin and MgO were combined, significant changes in the microbiota composition were observed compared with inulin alone.
33466274	2	49	from	drug	244:247	arg1	Japan					277:281	Japan	277:281	Japan	277:281	While magnesium oxide (MgO) is often used as the first-line drug for chronic constipation in Japan, dietary fiber intake is also recommended.
33466274	2	50	theme	magnesium	190:198	arg1	drug					244:247	the first-line drug	229:247	the first-line drug for chronic constipation in Japan	229:281	While magnesium oxide (MgO) is often used as the first-line drug for chronic constipation in Japan, dietary fiber intake is also recommended.
33466274	2	50	theme	magnesium	190:198	arg1	MgO					207:209	MgO	207:209	MgO	207:209	While magnesium oxide (MgO) is often used as the first-line drug for chronic constipation in Japan, dietary fiber intake is also recommended.
33466274	2	50	theme	magnesium	190:198	arg1	oxide					200:204	magnesium oxide	190:204	magnesium oxide (MgO)	190:210	While magnesium oxide (MgO) is often used as the first-line drug for chronic constipation in Japan, dietary fiber intake is also recommended.
33466274	2	51	theme	fiber	292:296	arg1	intake					298:303	dietary fiber intake	284:303	dietary fiber intake	284:303	While magnesium oxide (MgO) is often used as the first-line drug for chronic constipation in Japan, dietary fiber intake is also recommended.
33466274	10	52	theme	acid	1201:1204	arg1	concentration					1206:1218	the cecal acetic acid concentration	1184:1218	the cecal acetic acid concentration	1184:1218	The MgO effect on the cecal acetic acid concentration was less when administered at ZT12 than at ZT0.
33466274	11	53	from	effect	1377:1382	arg1	concentration					1399:1411	acetic acid concentration	1387:1411	acetic acid concentration	1387:1411	In conclusion, this study suggests that MgO affects cecal SCFA and microbiota during inulin feeding, and the effect on acetic acid concentration is time-dependent.
33466274	6	54	theme	cecal	693:697	arg1	SCFAs					699:703	cecal SCFAs	693:703	cecal SCFAs	693:703	We also examined the MgO administration timing effect on cecal SCFAs.
33466274	2	55	theme	dietary	284:290	arg1	intake					298:303	dietary fiber intake	284:303	dietary fiber intake	284:303	While magnesium oxide (MgO) is often used as the first-line drug for chronic constipation in Japan, dietary fiber intake is also recommended.
33466274	10	56	theme	acetic	1194:1199	arg1	acid					1201:1204	the cecal acetic acid	1184:1204	the cecal acetic acid concentration	1184:1218	The MgO effect on the cecal acetic acid concentration was less when administered at ZT12 than at ZT0.
33466274	7	57	theme	cecal	710:714	arg1	concentrations					721:734	The cecal SCFA concentrations	706:734	The cecal SCFA concentrations	706:734	The cecal SCFA concentrations were measured by gas chromatography, and the microbiota was determined using next-generation sequencing.
33466274	0	58	theme	Intestinal	54:63	arg1	Microbiota					65:74	Intestinal Microbiota	54:74	Intestinal Microbiota	54:74	The Combined Effects of Magnesium Oxide and Inulin on Intestinal Microbiota and Cecal Short-Chain Fatty Acids.
33466274	8	59	theme	combining	920:928	arg1	MgO					930:932	combining MgO	920:932	combining MgO	920:932	Inulin intake decreased cecal pH and increased cecal SCFA concentrations while combining MgO increased the cecal pH lowered by inulin and decreased the cecal SCFA concentrations elevated by inulin.
33466274	1	60	theme	common	129:134	arg1	condition					136:144	a common condition	127:144	a common condition that occurs in many people worldwide	127:181	Constipation is a common condition that occurs in many people worldwide.
33466274	1	60	theme	common	129:134	arg1	Constipation					111:122	Constipation	111:122	Constipation	111:122	Constipation is a common condition that occurs in many people worldwide.
33466274	10	61	theme	cecal	1188:1192	arg1	acid					1201:1204	the cecal acetic acid	1184:1204	the cecal acetic acid concentration	1184:1218	The MgO effect on the cecal acetic acid concentration was less when administered at ZT12 than at ZT0.
33466274	11	62	theme	cecal	1320:1324	arg1	SCFA					1326:1329	cecal SCFA	1320:1329	cecal SCFA	1320:1329	In conclusion, this study suggests that MgO affects cecal SCFA and microbiota during inulin feeding, and the effect on acetic acid concentration is time-dependent.
33466274	3	63	theme	Dietary	326:332	arg1	fiber					334:338	Dietary fiber	326:338	Dietary fiber	326:338	Dietary fiber is fermented by microbiota to produce short-chain fatty acids (SCFAs).
34693717	4	0	theme	0.66	884:887	arg1	±					897:897	±	897:897	±	897:897	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	4	1	theme	histopathological	908:924	arg1	changes					926:932	histopathological changes	908:932	histopathological changes	908:932	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	5	2	theme	DSS	1507:1509	arg1	treatment					1511:1519	DSS treatment	1507:1519	DSS treatment	1507:1519	Furthermore, dietary Hes-7-G not only profoundly regulated the gut microbiota composition including phyla Bacteroidetes, Cyanobacteria, Desulfobacterota, and Deferribacteres and genus Enterorhabdus, Prevotellaceae, Gastranaerophilales, Enterococcus, Intestinimonas, Ruminococcaceae, and Eubacterium in the cecal contents but also especially adjusted the co-metabolites such as short chain fatty acids and indole metabolites (indole-3-propionic, indole acetic acid), which were markedly altered by DSS treatment in mice.
34693717	4	3	theme	dietary	720:726	arg1	weight					750:755	1 mg/kg body weight	737:755	1 mg/kg body weight	737:755	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	4	3	theme	dietary	720:726	arg1	Hes-7-G					728:734	dietary Hes-7-G	720:734	dietary Hes-7-G (1 mg/kg body weight)	720:756	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	1	4	theme	high	289:292	arg1	activities					305:314	relatively high biological activities	278:314	relatively high biological activities	278:314	Hesperetin-7-O-glucoside (Hes-7-G) is a naturally occurring flavonoid monoglucoside in Citri Reticulatae Pericarpium and exhibits relatively high biological activities.
34693717	5	5	from	composition	1088:1098	arg1	contents					1322:1329	the cecal contents	1312:1329	the cecal contents	1312:1329	Furthermore, dietary Hes-7-G not only profoundly regulated the gut microbiota composition including phyla Bacteroidetes, Cyanobacteria, Desulfobacterota, and Deferribacteres and genus Enterorhabdus, Prevotellaceae, Gastranaerophilales, Enterococcus, Intestinimonas, Ruminococcaceae, and Eubacterium in the cecal contents but also especially adjusted the co-metabolites such as short chain fatty acids and indole metabolites (indole-3-propionic, indole acetic acid), which were markedly altered by DSS treatment in mice.
34693717	4	6	theme	DSS-induced	805:815	arg1	colitis					817:823	DSS-induced colitis	805:823	DSS-induced colitis mice	805:828	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	2	7	dep	in	508:509	arg1	vitro					511:515	vitro	511:515	vitro	511:515	To explore the anti-inflammatory capacity of dietary Hes-7-G, lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and dextran sodium sulfate (DSS)-induced colitis mice were used here as in vitro and in vivo inflammation models.
34693717	4	8	from	status	795:800	arg1	mice					825:828	DSS-induced colitis mice	805:828	DSS-induced colitis mice	805:828	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	3	9	theme	metabolic	621:629	arg1	disorders					631:639	cellular metabolic disorders	612:639	cellular metabolic disorders	612:639	The results showed that Hes-7-G (5 μM) significantly restored cellular metabolic disorders and inflammation in LPS-stimulated RAW264.7 macrophages.
34693717	4	10	dep	length	865:870	arg1	cm					904:905	cm	904:905	cm	904:905	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	1	11	theme	biological	294:303	arg1	activities					305:314	relatively high biological activities	278:314	relatively high biological activities	278:314	Hesperetin-7-O-glucoside (Hes-7-G) is a naturally occurring flavonoid monoglucoside in Citri Reticulatae Pericarpium and exhibits relatively high biological activities.
34693717	0	12	theme	Occurring	79:87	arg1	Monoglucoside					89:101	a Naturally Occurring Monoglucoside	67:101	a Naturally Occurring Monoglucoside	67:101	In Vitro and In Vivo Studies Reveal that Hesperetin-7-O-glucoside, a Naturally Occurring Monoglucoside, Exhibits Strong Anti-inflammatory Capacity.
34693717	0	12	theme	Occurring	79:87	arg1	Hesperetin-7-O-glucoside					41:64	Hesperetin-7-O-glucoside	41:64	Hesperetin-7-O-glucoside	41:64	In Vitro and In Vivo Studies Reveal that Hesperetin-7-O-glucoside, a Naturally Occurring Monoglucoside, Exhibits Strong Anti-inflammatory Capacity.
34693717	6	13	theme	anti-inflammatory	1582:1598	arg1	activity					1600:1607	strong anti-inflammatory activity	1575:1607	strong anti-inflammatory activity in vitro and in vivo	1575:1628	These findings demonstrated that Hes-7-G has strong anti-inflammatory activity in vitro and in vivo and potential preventive or therapeutic effects for chronic inflammation diseases.
34693717	4	14	theme	mg/kg	739:743	arg1	weight					750:755	1 mg/kg body weight	737:755	1 mg/kg body weight	737:755	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	4	14	theme	mg/kg	739:743	arg1	Hes-7-G					728:734	dietary Hes-7-G	720:734	dietary Hes-7-G (1 mg/kg body weight)	720:756	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	4	15	theme	colonic	954:960	arg1	factors					975:981	colonic inflammatory factors	954:981	colonic inflammatory factors including Tnf-α and Il-22	954:1007	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	4	15	theme	colonic	954:960	arg1	Il-22					1003:1007	Il-22	1003:1007	Il-22	1003:1007	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	4	15	theme	colonic	954:960	arg1	Tnf-α					993:997	Tnf-α	993:997	Tnf-α	993:997	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	6	16	theme	inflammation	1690:1701	arg1	diseases					1703:1710	chronic inflammation diseases	1682:1710	chronic inflammation diseases	1682:1710	These findings demonstrated that Hes-7-G has strong anti-inflammatory activity in vitro and in vivo and potential preventive or therapeutic effects for chronic inflammation diseases.
34693717	5	17	theme	short	1387:1391	arg1	acids					1405:1409	short chain fatty acids	1387:1409	short chain fatty acids	1387:1409	Furthermore, dietary Hes-7-G not only profoundly regulated the gut microbiota composition including phyla Bacteroidetes, Cyanobacteria, Desulfobacterota, and Deferribacteres and genus Enterorhabdus, Prevotellaceae, Gastranaerophilales, Enterococcus, Intestinimonas, Ruminococcaceae, and Eubacterium in the cecal contents but also especially adjusted the co-metabolites such as short chain fatty acids and indole metabolites (indole-3-propionic, indole acetic acid), which were markedly altered by DSS treatment in mice.
34693717	5	17	theme	short	1387:1391	arg1	acid					1469:1472	indole-3-propionic, indole acetic acid	1435:1472	indole-3-propionic, indole acetic acid	1435:1472	Furthermore, dietary Hes-7-G not only profoundly regulated the gut microbiota composition including phyla Bacteroidetes, Cyanobacteria, Desulfobacterota, and Deferribacteres and genus Enterorhabdus, Prevotellaceae, Gastranaerophilales, Enterococcus, Intestinimonas, Ruminococcaceae, and Eubacterium in the cecal contents but also especially adjusted the co-metabolites such as short chain fatty acids and indole metabolites (indole-3-propionic, indole acetic acid), which were markedly altered by DSS treatment in mice.
34693717	2	18	theme	inflammation	529:540	arg1	mice					485:488	dextran sodium sulfate (DSS)-induced colitis mice	440:488	dextran sodium sulfate (DSS)-induced colitis mice	440:488	To explore the anti-inflammatory capacity of dietary Hes-7-G, lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and dextran sodium sulfate (DSS)-induced colitis mice were used here as in vitro and in vivo inflammation models.
34693717	2	18	theme	inflammation	529:540	arg1	models					542:547	in vitro and in vivo inflammation models	508:547	in vitro and in vivo inflammation models	508:547	To explore the anti-inflammatory capacity of dietary Hes-7-G, lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and dextran sodium sulfate (DSS)-induced colitis mice were used here as in vitro and in vivo inflammation models.
34693717	2	18	theme	inflammation	529:540	arg1	macrophages					424:434	lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages	379:434	lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages	379:434	To explore the anti-inflammatory capacity of dietary Hes-7-G, lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and dextran sodium sulfate (DSS)-induced colitis mice were used here as in vitro and in vivo inflammation models.
34693717	4	19	theme	colon	859:863	arg1	length					865:870	the recovered colon length	845:870	the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm	845:905	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	5	20	theme	acetic	1462:1467	arg1	acids					1405:1409	short chain fatty acids	1387:1409	short chain fatty acids	1387:1409	Furthermore, dietary Hes-7-G not only profoundly regulated the gut microbiota composition including phyla Bacteroidetes, Cyanobacteria, Desulfobacterota, and Deferribacteres and genus Enterorhabdus, Prevotellaceae, Gastranaerophilales, Enterococcus, Intestinimonas, Ruminococcaceae, and Eubacterium in the cecal contents but also especially adjusted the co-metabolites such as short chain fatty acids and indole metabolites (indole-3-propionic, indole acetic acid), which were markedly altered by DSS treatment in mice.
34693717	5	20	theme	acetic	1462:1467	arg1	acid					1469:1472	indole-3-propionic, indole acetic acid	1435:1472	indole-3-propionic, indole acetic acid	1435:1472	Furthermore, dietary Hes-7-G not only profoundly regulated the gut microbiota composition including phyla Bacteroidetes, Cyanobacteria, Desulfobacterota, and Deferribacteres and genus Enterorhabdus, Prevotellaceae, Gastranaerophilales, Enterococcus, Intestinimonas, Ruminococcaceae, and Eubacterium in the cecal contents but also especially adjusted the co-metabolites such as short chain fatty acids and indole metabolites (indole-3-propionic, indole acetic acid), which were markedly altered by DSS treatment in mice.
34693717	4	21	dep	cm	904:905	arg1	±					897:897	±	897:897	±	897:897	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	5	22	theme	chain	1393:1397	arg1	acids					1405:1409	short chain fatty acids	1387:1409	short chain fatty acids	1387:1409	Furthermore, dietary Hes-7-G not only profoundly regulated the gut microbiota composition including phyla Bacteroidetes, Cyanobacteria, Desulfobacterota, and Deferribacteres and genus Enterorhabdus, Prevotellaceae, Gastranaerophilales, Enterococcus, Intestinimonas, Ruminococcaceae, and Eubacterium in the cecal contents but also especially adjusted the co-metabolites such as short chain fatty acids and indole metabolites (indole-3-propionic, indole acetic acid), which were markedly altered by DSS treatment in mice.
34693717	5	22	theme	chain	1393:1397	arg1	acid					1469:1472	indole-3-propionic, indole acetic acid	1435:1472	indole-3-propionic, indole acetic acid	1435:1472	Furthermore, dietary Hes-7-G not only profoundly regulated the gut microbiota composition including phyla Bacteroidetes, Cyanobacteria, Desulfobacterota, and Deferribacteres and genus Enterorhabdus, Prevotellaceae, Gastranaerophilales, Enterococcus, Intestinimonas, Ruminococcaceae, and Eubacterium in the cecal contents but also especially adjusted the co-metabolites such as short chain fatty acids and indole metabolites (indole-3-propionic, indole acetic acid), which were markedly altered by DSS treatment in mice.
34693717	5	23	theme	dietary	1023:1029	arg1	Hes-7-G					1031:1037	dietary Hes-7-G	1023:1037	dietary Hes-7-G	1023:1037	Furthermore, dietary Hes-7-G not only profoundly regulated the gut microbiota composition including phyla Bacteroidetes, Cyanobacteria, Desulfobacterota, and Deferribacteres and genus Enterorhabdus, Prevotellaceae, Gastranaerophilales, Enterococcus, Intestinimonas, Ruminococcaceae, and Eubacterium in the cecal contents but also especially adjusted the co-metabolites such as short chain fatty acids and indole metabolites (indole-3-propionic, indole acetic acid), which were markedly altered by DSS treatment in mice.
34693717	4	24	dep	±	897:897	arg1	to					889:890	to	889:890	to	889:890	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	2	25	theme	dietary	362:368	arg1	Hes-7-G					370:376	dietary Hes-7-G	362:376	dietary Hes-7-G	362:376	To explore the anti-inflammatory capacity of dietary Hes-7-G, lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and dextran sodium sulfate (DSS)-induced colitis mice were used here as in vitro and in vivo inflammation models.
34693717	5	26	theme	fatty	1399:1403	arg1	acids					1405:1409	short chain fatty acids	1387:1409	short chain fatty acids	1387:1409	Furthermore, dietary Hes-7-G not only profoundly regulated the gut microbiota composition including phyla Bacteroidetes, Cyanobacteria, Desulfobacterota, and Deferribacteres and genus Enterorhabdus, Prevotellaceae, Gastranaerophilales, Enterococcus, Intestinimonas, Ruminococcaceae, and Eubacterium in the cecal contents but also especially adjusted the co-metabolites such as short chain fatty acids and indole metabolites (indole-3-propionic, indole acetic acid), which were markedly altered by DSS treatment in mice.
34693717	5	26	theme	fatty	1399:1403	arg1	acid					1469:1472	indole-3-propionic, indole acetic acid	1435:1472	indole-3-propionic, indole acetic acid	1435:1472	Furthermore, dietary Hes-7-G not only profoundly regulated the gut microbiota composition including phyla Bacteroidetes, Cyanobacteria, Desulfobacterota, and Deferribacteres and genus Enterorhabdus, Prevotellaceae, Gastranaerophilales, Enterococcus, Intestinimonas, Ruminococcaceae, and Eubacterium in the cecal contents but also especially adjusted the co-metabolites such as short chain fatty acids and indole metabolites (indole-3-propionic, indole acetic acid), which were markedly altered by DSS treatment in mice.
34693717	6	27	theme	strong	1575:1580	arg1	activity					1600:1607	strong anti-inflammatory activity	1575:1607	strong anti-inflammatory activity in vitro and in vivo	1575:1628	These findings demonstrated that Hes-7-G has strong anti-inflammatory activity in vitro and in vivo and potential preventive or therapeutic effects for chronic inflammation diseases.
34693717	0	28	theme	In	0:1	arg1	Studies					21:27	In Vitro and In Vivo Studies	0:27	In Vitro and In Vivo Studies	0:27	In Vitro and In Vivo Studies Reveal that Hesperetin-7-O-glucoside, a Naturally Occurring Monoglucoside, Exhibits Strong Anti-inflammatory Capacity.
34693717	6	29	contain	has	1571:1573	arg1	Hes-7-G					1563:1569	Hes-7-G	1563:1569	Hes-7-G	1563:1569	These findings demonstrated that Hes-7-G has strong anti-inflammatory activity in vitro and in vivo and potential preventive or therapeutic effects for chronic inflammation diseases.
34693717	6	29	contain	has	1571:1573	arg2	activity					1600:1607	strong anti-inflammatory activity	1575:1607	strong anti-inflammatory activity in vitro and in vivo	1575:1628	These findings demonstrated that Hes-7-G has strong anti-inflammatory activity in vitro and in vivo and potential preventive or therapeutic effects for chronic inflammation diseases.
34693717	6	29	contain	has	1571:1573	arg2	effects					1670:1676	potential preventive or therapeutic effects	1634:1676	potential preventive or therapeutic effects for chronic inflammation diseases	1634:1710	These findings demonstrated that Hes-7-G has strong anti-inflammatory activity in vitro and in vivo and potential preventive or therapeutic effects for chronic inflammation diseases.
34693717	1	30	theme	occurring	198:206	arg1	monoglucoside					218:230	a naturally occurring flavonoid monoglucoside	186:230	a naturally occurring flavonoid monoglucoside in Citri Reticulatae Pericarpium	186:263	Hesperetin-7-O-glucoside (Hes-7-G) is a naturally occurring flavonoid monoglucoside in Citri Reticulatae Pericarpium and exhibits relatively high biological activities.
34693717	1	30	theme	occurring	198:206	arg1	Hesperetin-7-O-glucoside					148:171	Hesperetin-7-O-glucoside	148:171	Hesperetin-7-O-glucoside (Hes-7-G)	148:181	Hesperetin-7-O-glucoside (Hes-7-G) is a naturally occurring flavonoid monoglucoside in Citri Reticulatae Pericarpium and exhibits relatively high biological activities.
34693717	2	31	theme	colitis	477:483	arg1	mice					485:488	dextran sodium sulfate (DSS)-induced colitis mice	440:488	dextran sodium sulfate (DSS)-induced colitis mice	440:488	To explore the anti-inflammatory capacity of dietary Hes-7-G, lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and dextran sodium sulfate (DSS)-induced colitis mice were used here as in vitro and in vivo inflammation models.
34693717	2	31	theme	colitis	477:483	arg1	models					542:547	in vitro and in vivo inflammation models	508:547	in vitro and in vivo inflammation models	508:547	To explore the anti-inflammatory capacity of dietary Hes-7-G, lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and dextran sodium sulfate (DSS)-induced colitis mice were used here as in vitro and in vivo inflammation models.
34693717	2	31	theme	colitis	477:483	arg1	macrophages					424:434	lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages	379:434	lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages	379:434	To explore the anti-inflammatory capacity of dietary Hes-7-G, lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and dextran sodium sulfate (DSS)-induced colitis mice were used here as in vitro and in vivo inflammation models.
34693717	0	32	theme	Anti-inflammatory	120:136	arg1	Capacity					138:145	Strong Anti-inflammatory Capacity	113:145	Strong Anti-inflammatory Capacity	113:145	In Vitro and In Vivo Studies Reveal that Hesperetin-7-O-glucoside, a Naturally Occurring Monoglucoside, Exhibits Strong Anti-inflammatory Capacity.
34693717	6	33	theme	therapeutic	1658:1668	arg1	effects					1670:1676	potential preventive or therapeutic effects	1634:1676	potential preventive or therapeutic effects for chronic inflammation diseases	1634:1710	These findings demonstrated that Hes-7-G has strong anti-inflammatory activity in vitro and in vivo and potential preventive or therapeutic effects for chronic inflammation diseases.
34693717	1	34	theme	flavonoid	208:216	arg1	monoglucoside					218:230	a naturally occurring flavonoid monoglucoside	186:230	a naturally occurring flavonoid monoglucoside in Citri Reticulatae Pericarpium	186:263	Hesperetin-7-O-glucoside (Hes-7-G) is a naturally occurring flavonoid monoglucoside in Citri Reticulatae Pericarpium and exhibits relatively high biological activities.
34693717	1	34	theme	flavonoid	208:216	arg1	Hesperetin-7-O-glucoside					148:171	Hesperetin-7-O-glucoside	148:171	Hesperetin-7-O-glucoside (Hes-7-G)	148:181	Hesperetin-7-O-glucoside (Hes-7-G) is a naturally occurring flavonoid monoglucoside in Citri Reticulatae Pericarpium and exhibits relatively high biological activities.
34693717	2	35	theme	-induced	468:475	arg1	colitis					477:483	dextran sodium sulfate (DSS)-induced colitis	440:483	dextran sodium sulfate (DSS)-induced colitis mice	440:488	To explore the anti-inflammatory capacity of dietary Hes-7-G, lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and dextran sodium sulfate (DSS)-induced colitis mice were used here as in vitro and in vivo inflammation models.
34693717	0	36	theme	Strong	113:118	arg1	Capacity					138:145	Strong Anti-inflammatory Capacity	113:145	Strong Anti-inflammatory Capacity	113:145	In Vitro and In Vivo Studies Reveal that Hesperetin-7-O-glucoside, a Naturally Occurring Monoglucoside, Exhibits Strong Anti-inflammatory Capacity.
34693717	5	37	theme	gut	1073:1075	arg1	composition					1088:1098	the gut microbiota composition	1069:1098	the gut microbiota composition including phyla Bacteroidetes, Cyanobacteria, Desulfobacterota, and Deferribacteres and genus Enterorhabdus, Prevotellaceae, Gastranaerophilales, Enterococcus, Intestinimonas, Ruminococcaceae, and Eubacterium in the cecal contents	1069:1329	Furthermore, dietary Hes-7-G not only profoundly regulated the gut microbiota composition including phyla Bacteroidetes, Cyanobacteria, Desulfobacterota, and Deferribacteres and genus Enterorhabdus, Prevotellaceae, Gastranaerophilales, Enterococcus, Intestinimonas, Ruminococcaceae, and Eubacterium in the cecal contents but also especially adjusted the co-metabolites such as short chain fatty acids and indole metabolites (indole-3-propionic, indole acetic acid), which were markedly altered by DSS treatment in mice.
34693717	2	38	theme	anti-inflammatory	332:348	arg1	capacity					350:357	the anti-inflammatory capacity	328:357	the anti-inflammatory capacity of dietary Hes-7-G	328:376	To explore the anti-inflammatory capacity of dietary Hes-7-G, lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and dextran sodium sulfate (DSS)-induced colitis mice were used here as in vitro and in vivo inflammation models.
34693717	6	39	theme	chronic	1682:1688	arg1	diseases					1703:1710	chronic inflammation diseases	1682:1710	chronic inflammation diseases	1682:1710	These findings demonstrated that Hes-7-G has strong anti-inflammatory activity in vitro and in vivo and potential preventive or therapeutic effects for chronic inflammation diseases.
34693717	5	40	dep	indole-3-propionic	1435:1452	arg1	indole					1455:1460	indole	1455:1460	indole	1455:1460	Furthermore, dietary Hes-7-G not only profoundly regulated the gut microbiota composition including phyla Bacteroidetes, Cyanobacteria, Desulfobacterota, and Deferribacteres and genus Enterorhabdus, Prevotellaceae, Gastranaerophilales, Enterococcus, Intestinimonas, Ruminococcaceae, and Eubacterium in the cecal contents but also especially adjusted the co-metabolites such as short chain fatty acids and indole metabolites (indole-3-propionic, indole acetic acid), which were markedly altered by DSS treatment in mice.
34693717	2	41	theme	in	508:509	arg1	mice					485:488	dextran sodium sulfate (DSS)-induced colitis mice	440:488	dextran sodium sulfate (DSS)-induced colitis mice	440:488	To explore the anti-inflammatory capacity of dietary Hes-7-G, lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and dextran sodium sulfate (DSS)-induced colitis mice were used here as in vitro and in vivo inflammation models.
34693717	2	41	theme	in	508:509	arg1	models					542:547	in vitro and in vivo inflammation models	508:547	in vitro and in vivo inflammation models	508:547	To explore the anti-inflammatory capacity of dietary Hes-7-G, lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and dextran sodium sulfate (DSS)-induced colitis mice were used here as in vitro and in vivo inflammation models.
34693717	2	41	theme	in	508:509	arg1	macrophages					424:434	lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages	379:434	lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages	379:434	To explore the anti-inflammatory capacity of dietary Hes-7-G, lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and dextran sodium sulfate (DSS)-induced colitis mice were used here as in vitro and in vivo inflammation models.
34693717	5	42	theme	microbiota	1077:1086	arg1	composition					1088:1098	the gut microbiota composition	1069:1098	the gut microbiota composition including phyla Bacteroidetes, Cyanobacteria, Desulfobacterota, and Deferribacteres and genus Enterorhabdus, Prevotellaceae, Gastranaerophilales, Enterococcus, Intestinimonas, Ruminococcaceae, and Eubacterium in the cecal contents	1069:1329	Furthermore, dietary Hes-7-G not only profoundly regulated the gut microbiota composition including phyla Bacteroidetes, Cyanobacteria, Desulfobacterota, and Deferribacteres and genus Enterorhabdus, Prevotellaceae, Gastranaerophilales, Enterococcus, Intestinimonas, Ruminococcaceae, and Eubacterium in the cecal contents but also especially adjusted the co-metabolites such as short chain fatty acids and indole metabolites (indole-3-propionic, indole acetic acid), which were markedly altered by DSS treatment in mice.
34693717	3	43	theme	cellular	612:619	arg1	disorders					631:639	cellular metabolic disorders	612:639	cellular metabolic disorders	612:639	The results showed that Hes-7-G (5 μM) significantly restored cellular metabolic disorders and inflammation in LPS-stimulated RAW264.7 macrophages.
34693717	4	44	theme	in	705:706	arg1	study					713:717	the in vivo study	701:717	the in vivo study	701:717	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	6	45	theme	preventive	1644:1653	arg1	effects					1670:1676	potential preventive or therapeutic effects	1634:1676	potential preventive or therapeutic effects for chronic inflammation diseases	1634:1710	These findings demonstrated that Hes-7-G has strong anti-inflammatory activity in vitro and in vivo and potential preventive or therapeutic effects for chronic inflammation diseases.
34693717	3	46	theme	LPS-stimulated	661:674	arg1	macrophages					685:695	LPS-stimulated RAW264.7 macrophages	661:695	LPS-stimulated RAW264.7 macrophages	661:695	The results showed that Hes-7-G (5 μM) significantly restored cellular metabolic disorders and inflammation in LPS-stimulated RAW264.7 macrophages.
34693717	0	47	theme	In	13:14	arg1	Studies					21:27	In Vitro and In Vivo Studies	0:27	In Vitro and In Vivo Studies	0:27	In Vitro and In Vivo Studies Reveal that Hesperetin-7-O-glucoside, a Naturally Occurring Monoglucoside, Exhibits Strong Anti-inflammatory Capacity.
34693717	4	48	dep	in	705:706	arg1	vivo					708:711	vivo	708:711	vivo	708:711	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	4	49	theme	0.17	899:902	arg1	±					897:897	±	897:897	±	897:897	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	3	50	theme	RAW264.7	676:683	arg1	macrophages					685:695	LPS-stimulated RAW264.7 macrophages	661:695	LPS-stimulated RAW264.7 macrophages	661:695	The results showed that Hes-7-G (5 μM) significantly restored cellular metabolic disorders and inflammation in LPS-stimulated RAW264.7 macrophages.
34693717	4	51	theme	recovered	849:857	arg1	length					865:870	the recovered colon length	845:870	the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm	845:905	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	5	52	theme	indole-3-propionic	1435:1452	arg1	acids					1405:1409	short chain fatty acids	1387:1409	short chain fatty acids	1387:1409	Furthermore, dietary Hes-7-G not only profoundly regulated the gut microbiota composition including phyla Bacteroidetes, Cyanobacteria, Desulfobacterota, and Deferribacteres and genus Enterorhabdus, Prevotellaceae, Gastranaerophilales, Enterococcus, Intestinimonas, Ruminococcaceae, and Eubacterium in the cecal contents but also especially adjusted the co-metabolites such as short chain fatty acids and indole metabolites (indole-3-propionic, indole acetic acid), which were markedly altered by DSS treatment in mice.
34693717	5	52	theme	indole-3-propionic	1435:1452	arg1	acid					1469:1472	indole-3-propionic, indole acetic acid	1435:1472	indole-3-propionic, indole acetic acid	1435:1472	Furthermore, dietary Hes-7-G not only profoundly regulated the gut microbiota composition including phyla Bacteroidetes, Cyanobacteria, Desulfobacterota, and Deferribacteres and genus Enterorhabdus, Prevotellaceae, Gastranaerophilales, Enterococcus, Intestinimonas, Ruminococcaceae, and Eubacterium in the cecal contents but also especially adjusted the co-metabolites such as short chain fatty acids and indole metabolites (indole-3-propionic, indole acetic acid), which were markedly altered by DSS treatment in mice.
34693717	4	53	from	±	882:882	arg1	changes					926:932	histopathological changes	908:932	histopathological changes	908:932	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	4	53	from	±	882:882	arg1	length					865:870	the recovered colon length	845:870	the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm	845:905	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	4	53	from	±	882:882	arg1	levels					944:949	mRNA levels	939:949	mRNA levels of colonic inflammatory factors including Tnf-α and Il-22	939:1007	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	5	54	dep	phyla	1110:1114	arg1	Bacteroidetes					1116:1128	phyla Bacteroidetes	1110:1128	phyla Bacteroidetes	1110:1128	Furthermore, dietary Hes-7-G not only profoundly regulated the gut microbiota composition including phyla Bacteroidetes, Cyanobacteria, Desulfobacterota, and Deferribacteres and genus Enterorhabdus, Prevotellaceae, Gastranaerophilales, Enterococcus, Intestinimonas, Ruminococcaceae, and Eubacterium in the cecal contents but also especially adjusted the co-metabolites such as short chain fatty acids and indole metabolites (indole-3-propionic, indole acetic acid), which were markedly altered by DSS treatment in mice.
34693717	4	55	theme	inflammatory	962:973	arg1	factors					975:981	colonic inflammatory factors	954:981	colonic inflammatory factors including Tnf-α and Il-22	954:1007	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	4	55	theme	inflammatory	962:973	arg1	Il-22					1003:1007	Il-22	1003:1007	Il-22	1003:1007	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	4	55	theme	inflammatory	962:973	arg1	Tnf-α					993:997	Tnf-α	993:997	Tnf-α	993:997	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	6	56	theme	potential	1634:1642	arg1	effects					1670:1676	potential preventive or therapeutic effects	1634:1676	potential preventive or therapeutic effects for chronic inflammation diseases	1634:1710	These findings demonstrated that Hes-7-G has strong anti-inflammatory activity in vitro and in vivo and potential preventive or therapeutic effects for chronic inflammation diseases.
34693717	4	57	theme	6.45	892:895	arg1	±					897:897	±	897:897	±	897:897	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	4	58	theme	colitis	817:823	arg1	mice					825:828	DSS-induced colitis mice	805:828	DSS-induced colitis mice	805:828	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	0	59	dep	In	13:14	arg1	Vivo					16:19	Vivo	16:19	Vivo	16:19	In Vitro and In Vivo Studies Reveal that Hesperetin-7-O-glucoside, a Naturally Occurring Monoglucoside, Exhibits Strong Anti-inflammatory Capacity.
34693717	1	60	from	monoglucoside	218:230	arg1	Pericarpium					253:263	Citri Reticulatae Pericarpium	235:263	Citri Reticulatae Pericarpium	235:263	Hesperetin-7-O-glucoside (Hes-7-G) is a naturally occurring flavonoid monoglucoside in Citri Reticulatae Pericarpium and exhibits relatively high biological activities.
34693717	4	61	theme	body	745:748	arg1	weight					750:755	1 mg/kg body weight	737:755	1 mg/kg body weight	737:755	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	4	61	theme	body	745:748	arg1	Hes-7-G					728:734	dietary Hes-7-G	720:734	dietary Hes-7-G (1 mg/kg body weight)	720:756	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	2	62	dep	in	521:522	arg1	vivo					524:527	vivo	524:527	vivo	524:527	To explore the anti-inflammatory capacity of dietary Hes-7-G, lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and dextran sodium sulfate (DSS)-induced colitis mice were used here as in vitro and in vivo inflammation models.
34693717	4	63	theme	inflammatory	782:793	arg1	status					795:800	the inflammatory status	778:800	the inflammatory status in DSS-induced colitis mice	778:828	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	2	64	theme	Hes-7-G	370:376	arg1	capacity					350:357	the anti-inflammatory capacity	328:357	the anti-inflammatory capacity of dietary Hes-7-G	328:376	To explore the anti-inflammatory capacity of dietary Hes-7-G, lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and dextran sodium sulfate (DSS)-induced colitis mice were used here as in vitro and in vivo inflammation models.
34693717	5	65	dep	Ruminococcaceae	1276:1290	arg1	Intestinimonas					1260:1273	Intestinimonas	1260:1273	Intestinimonas	1260:1273	Furthermore, dietary Hes-7-G not only profoundly regulated the gut microbiota composition including phyla Bacteroidetes, Cyanobacteria, Desulfobacterota, and Deferribacteres and genus Enterorhabdus, Prevotellaceae, Gastranaerophilales, Enterococcus, Intestinimonas, Ruminococcaceae, and Eubacterium in the cecal contents but also especially adjusted the co-metabolites such as short chain fatty acids and indole metabolites (indole-3-propionic, indole acetic acid), which were markedly altered by DSS treatment in mice.
34693717	4	66	theme	factors	975:981	arg1	changes					926:932	histopathological changes	908:932	histopathological changes	908:932	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	4	66	theme	factors	975:981	arg1	length					865:870	the recovered colon length	845:870	the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm	845:905	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	4	66	theme	factors	975:981	arg1	levels					944:949	mRNA levels	939:949	mRNA levels of colonic inflammatory factors including Tnf-α and Il-22	939:1007	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
34693717	5	67	theme	cecal	1316:1320	arg1	contents					1322:1329	the cecal contents	1312:1329	the cecal contents	1312:1329	Furthermore, dietary Hes-7-G not only profoundly regulated the gut microbiota composition including phyla Bacteroidetes, Cyanobacteria, Desulfobacterota, and Deferribacteres and genus Enterorhabdus, Prevotellaceae, Gastranaerophilales, Enterococcus, Intestinimonas, Ruminococcaceae, and Eubacterium in the cecal contents but also especially adjusted the co-metabolites such as short chain fatty acids and indole metabolites (indole-3-propionic, indole acetic acid), which were markedly altered by DSS treatment in mice.
34693717	2	68	theme	RAW264.7	415:422	arg1	mice					485:488	dextran sodium sulfate (DSS)-induced colitis mice	440:488	dextran sodium sulfate (DSS)-induced colitis mice	440:488	To explore the anti-inflammatory capacity of dietary Hes-7-G, lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and dextran sodium sulfate (DSS)-induced colitis mice were used here as in vitro and in vivo inflammation models.
34693717	2	68	theme	RAW264.7	415:422	arg1	models					542:547	in vitro and in vivo inflammation models	508:547	in vitro and in vivo inflammation models	508:547	To explore the anti-inflammatory capacity of dietary Hes-7-G, lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and dextran sodium sulfate (DSS)-induced colitis mice were used here as in vitro and in vivo inflammation models.
34693717	2	68	theme	RAW264.7	415:422	arg1	macrophages					424:434	lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages	379:434	lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages	379:434	To explore the anti-inflammatory capacity of dietary Hes-7-G, lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and dextran sodium sulfate (DSS)-induced colitis mice were used here as in vitro and in vivo inflammation models.
34693717	2	69	theme	in	521:522	arg1	mice					485:488	dextran sodium sulfate (DSS)-induced colitis mice	440:488	dextran sodium sulfate (DSS)-induced colitis mice	440:488	To explore the anti-inflammatory capacity of dietary Hes-7-G, lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and dextran sodium sulfate (DSS)-induced colitis mice were used here as in vitro and in vivo inflammation models.
34693717	2	69	theme	in	521:522	arg1	models					542:547	in vitro and in vivo inflammation models	508:547	in vitro and in vivo inflammation models	508:547	To explore the anti-inflammatory capacity of dietary Hes-7-G, lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and dextran sodium sulfate (DSS)-induced colitis mice were used here as in vitro and in vivo inflammation models.
34693717	2	69	theme	in	521:522	arg1	macrophages					424:434	lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages	379:434	lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages	379:434	To explore the anti-inflammatory capacity of dietary Hes-7-G, lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and dextran sodium sulfate (DSS)-induced colitis mice were used here as in vitro and in vivo inflammation models.
34693717	2	70	used	used	495:498	arg2	macrophages					424:434	lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages	379:434	lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages	379:434	To explore the anti-inflammatory capacity of dietary Hes-7-G, lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and dextran sodium sulfate (DSS)-induced colitis mice were used here as in vitro and in vivo inflammation models.
34693717	2	70	used	used	495:498	arg2	models					542:547	in vitro and in vivo inflammation models	508:547	in vitro and in vivo inflammation models	508:547	To explore the anti-inflammatory capacity of dietary Hes-7-G, lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and dextran sodium sulfate (DSS)-induced colitis mice were used here as in vitro and in vivo inflammation models.
34693717	2	70	used	used	495:498	arg2	mice					485:488	dextran sodium sulfate (DSS)-induced colitis mice	440:488	dextran sodium sulfate (DSS)-induced colitis mice	440:488	To explore the anti-inflammatory capacity of dietary Hes-7-G, lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and dextran sodium sulfate (DSS)-induced colitis mice were used here as in vitro and in vivo inflammation models.
34693717	0	71	dep	In	0:1	arg1	Vitro					3:7	Vitro	3:7	Vitro	3:7	In Vitro and In Vivo Studies Reveal that Hesperetin-7-O-glucoside, a Naturally Occurring Monoglucoside, Exhibits Strong Anti-inflammatory Capacity.
34693717	5	72	theme	indole	1415:1420	arg1	metabolites					1422:1432	indole metabolites	1415:1432	indole metabolites	1415:1432	Furthermore, dietary Hes-7-G not only profoundly regulated the gut microbiota composition including phyla Bacteroidetes, Cyanobacteria, Desulfobacterota, and Deferribacteres and genus Enterorhabdus, Prevotellaceae, Gastranaerophilales, Enterococcus, Intestinimonas, Ruminococcaceae, and Eubacterium in the cecal contents but also especially adjusted the co-metabolites such as short chain fatty acids and indole metabolites (indole-3-propionic, indole acetic acid), which were markedly altered by DSS treatment in mice.
34693717	2	73	theme	-stimulated	403:413	arg1	mice					485:488	dextran sodium sulfate (DSS)-induced colitis mice	440:488	dextran sodium sulfate (DSS)-induced colitis mice	440:488	To explore the anti-inflammatory capacity of dietary Hes-7-G, lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and dextran sodium sulfate (DSS)-induced colitis mice were used here as in vitro and in vivo inflammation models.
34693717	2	73	theme	-stimulated	403:413	arg1	models					542:547	in vitro and in vivo inflammation models	508:547	in vitro and in vivo inflammation models	508:547	To explore the anti-inflammatory capacity of dietary Hes-7-G, lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and dextran sodium sulfate (DSS)-induced colitis mice were used here as in vitro and in vivo inflammation models.
34693717	2	73	theme	-stimulated	403:413	arg1	macrophages					424:434	lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages	379:434	lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages	379:434	To explore the anti-inflammatory capacity of dietary Hes-7-G, lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and dextran sodium sulfate (DSS)-induced colitis mice were used here as in vitro and in vivo inflammation models.
34693717	4	74	theme	mRNA	939:942	arg1	levels					944:949	mRNA levels	939:949	mRNA levels of colonic inflammatory factors including Tnf-α and Il-22	939:1007	In the in vivo study, dietary Hes-7-G (1 mg/kg body weight) markedly alleviated the inflammatory status in DSS-induced colitis mice, manifested by the recovered colon length from 5.91 ± 0.66 to 6.45 ± 0.17 cm, histopathological changes, and mRNA levels of colonic inflammatory factors including Tnf-α and Il-22.
32822899	2	0	theme	-based	530:535	arg1	metabolomics					537:548	-based metabolomics	530:548	-based metabolomics	530:548	In this regard, the phytochemical composition and variability of various morphotypes of acorns from Quercus ilex have been determined by using two platforms, near infrared spectroscopy (NIRS) and mass spectrometry (MS)-based metabolomics.
32822899	5	1	theme	highest	828:834	arg1	load					836:839	the highest load	824:839	the highest load	824:839	Principal component analysis (PCA) discriminated the morphotypes; 50 compounds out of 192 identified had the highest load over the first two PCA components (explaining 67.2% variability) and can be potential markers of variability.
32822899	0	2	from	composition	14:24	arg1	morphotypes					64:74	Quercus ilex acorn morphotypes	45:74	Quercus ilex acorn morphotypes	45:74	Phytochemical composition and variability in Quercus ilex acorn morphotypes as determined by NIRS and MS-based approaches.
32822899	4	3	used	used	642:645	arg2	analysis					629:636	UHPLC-QToF analysis	618:636	UHPLC-QToF analysis	618:636	UHPLC-QToF analysis was used for untargeted metabolomic analysis and 192 metabolites were annotated.
32822899	2	4	dep	spectroscopy	484:495	arg1	metabolomics					537:548	-based metabolomics	530:548	-based metabolomics	530:548	In this regard, the phytochemical composition and variability of various morphotypes of acorns from Quercus ilex have been determined by using two platforms, near infrared spectroscopy (NIRS) and mass spectrometry (MS)-based metabolomics.
32822899	1	5	theme	consumable	294:303	arg1	flour					305:309	consumable flour	294:309	consumable flour	294:309	Quercus acorns have been used as a staple food since archeological times; currently, there is a renewed interest in the direct use of the acorns as nuts or as a source of consumable flour.
32822899	6	6	theme	other	1095:1099	arg1	nuts					1101:1104	other nuts	1095:1104	other nuts	1095:1104	The data presented here support the value of acorns as a source of nutritionally rich compounds thus justifying their use a good alternative to other nuts.
32822899	5	7	theme	PCA	860:862	arg1	components					864:873	the first two PCA components	846:873	the first two PCA components (explaining 67.2% variability)	846:904	Principal component analysis (PCA) discriminated the morphotypes; 50 compounds out of 192 identified had the highest load over the first two PCA components (explaining 67.2% variability) and can be potential markers of variability.
32822899	1	8	theme	flour	305:309	arg1	source					284:289	a source	282:289	a source of consumable flour	282:309	Quercus acorns have been used as a staple food since archeological times; currently, there is a renewed interest in the direct use of the acorns as nuts or as a source of consumable flour.
32822899	1	9	theme	direct	243:248	arg1	use					250:252	the direct use	239:252	the direct use of the acorns as nuts or as a source of consumable flour	239:309	Quercus acorns have been used as a staple food since archeological times; currently, there is a renewed interest in the direct use of the acorns as nuts or as a source of consumable flour.
32822899	6	10	dep	justifying	1052:1061	arg1	use					1069:1071	their use	1063:1071	their use	1063:1071	The data presented here support the value of acorns as a source of nutritionally rich compounds thus justifying their use a good alternative to other nuts.
32822899	1	11	used	used	148:151	arg2	food					165:168	a staple food	156:168	a staple food	156:168	Quercus acorns have been used as a staple food since archeological times; currently, there is a renewed interest in the direct use of the acorns as nuts or as a source of consumable flour.
32822899	1	11	used	used	148:151	arg2	acorns					131:136	Quercus acorns	123:136	Quercus acorns	123:136	Quercus acorns have been used as a staple food since archeological times; currently, there is a renewed interest in the direct use of the acorns as nuts or as a source of consumable flour.
32822899	1	12	theme	staple	158:163	arg1	acorns					131:136	Quercus acorns	123:136	Quercus acorns	123:136	Quercus acorns have been used as a staple food since archeological times; currently, there is a renewed interest in the direct use of the acorns as nuts or as a source of consumable flour.
32822899	1	12	theme	staple	158:163	arg1	food					165:168	a staple food	156:168	a staple food	156:168	Quercus acorns have been used as a staple food since archeological times; currently, there is a renewed interest in the direct use of the acorns as nuts or as a source of consumable flour.
32822899	0	13	theme	MS-based	102:109	arg1	approaches					111:120	MS-based approaches	102:120	MS-based approaches	102:120	Phytochemical composition and variability in Quercus ilex acorn morphotypes as determined by NIRS and MS-based approaches.
32822899	4	14	theme	192	687:689	arg1	metabolites					691:701	192 metabolites	687:701	192 metabolites	687:701	UHPLC-QToF analysis was used for untargeted metabolomic analysis and 192 metabolites were annotated.
32822899	2	15	theme	acorns	400:405	arg1	morphotypes					385:395	various morphotypes	377:395	various morphotypes of acorns from Quercus ilex	377:423	In this regard, the phytochemical composition and variability of various morphotypes of acorns from Quercus ilex have been determined by using two platforms, near infrared spectroscopy (NIRS) and mass spectrometry (MS)-based metabolomics.
32822899	2	16	from	ilex	420:423	arg1	acorns					400:405	acorns	400:405	acorns from Quercus ilex	400:423	In this regard, the phytochemical composition and variability of various morphotypes of acorns from Quercus ilex have been determined by using two platforms, near infrared spectroscopy (NIRS) and mass spectrometry (MS)-based metabolomics.
32822899	2	16	from	ilex	420:423	arg1	morphotypes					385:395	various morphotypes	377:395	various morphotypes of acorns from Quercus ilex	377:423	In this regard, the phytochemical composition and variability of various morphotypes of acorns from Quercus ilex have been determined by using two platforms, near infrared spectroscopy (NIRS) and mass spectrometry (MS)-based metabolomics.
32822899	0	17	theme	Phytochemical	0:12	arg1	composition					14:24	Phytochemical composition	0:24	Phytochemical composition	0:24	Phytochemical composition and variability in Quercus ilex acorn morphotypes as determined by NIRS and MS-based approaches.
32822899	5	18	theme	first	850:854	arg1	components					864:873	the first two PCA components	846:873	the first two PCA components (explaining 67.2% variability)	846:904	Principal component analysis (PCA) discriminated the morphotypes; 50 compounds out of 192 identified had the highest load over the first two PCA components (explaining 67.2% variability) and can be potential markers of variability.
32822899	1	19	theme	archeological	176:188	arg1	times					190:194	archeological times	176:194	archeological times	176:194	Quercus acorns have been used as a staple food since archeological times; currently, there is a renewed interest in the direct use of the acorns as nuts or as a source of consumable flour.
32822899	2	20	theme	morphotypes	385:395	arg1	variability					362:372	variability	362:372	variability	362:372	In this regard, the phytochemical composition and variability of various morphotypes of acorns from Quercus ilex have been determined by using two platforms, near infrared spectroscopy (NIRS) and mass spectrometry (MS)-based metabolomics.
32822899	2	20	theme	morphotypes	385:395	arg1	composition					346:356	phytochemical composition	332:356	phytochemical composition	332:356	In this regard, the phytochemical composition and variability of various morphotypes of acorns from Quercus ilex have been determined by using two platforms, near infrared spectroscopy (NIRS) and mass spectrometry (MS)-based metabolomics.
32822899	4	21	theme	metabolomic	662:672	arg1	analysis					674:681	untargeted metabolomic analysis	651:681	untargeted metabolomic analysis	651:681	UHPLC-QToF analysis was used for untargeted metabolomic analysis and 192 metabolites were annotated.
32822899	2	22	from	morphotypes	385:395	arg1	ilex					420:423	Quercus ilex	412:423	Quercus ilex	412:423	In this regard, the phytochemical composition and variability of various morphotypes of acorns from Quercus ilex have been determined by using two platforms, near infrared spectroscopy (NIRS) and mass spectrometry (MS)-based metabolomics.
32822899	2	23	theme	various	377:383	arg1	morphotypes					385:395	various morphotypes	377:395	various morphotypes of acorns from Quercus ilex	377:423	In this regard, the phytochemical composition and variability of various morphotypes of acorns from Quercus ilex have been determined by using two platforms, near infrared spectroscopy (NIRS) and mass spectrometry (MS)-based metabolomics.
32822899	4	24	theme	untargeted	651:660	arg1	analysis					674:681	untargeted metabolomic analysis	651:681	untargeted metabolomic analysis	651:681	UHPLC-QToF analysis was used for untargeted metabolomic analysis and 192 metabolites were annotated.
32822899	6	25	theme	compounds	1037:1045	arg1	source					1008:1013	a source	1006:1013	a source of nutritionally rich compounds	1006:1045	The data presented here support the value of acorns as a source of nutritionally rich compounds thus justifying their use a good alternative to other nuts.
32822899	6	25	theme	compounds	1037:1045	arg1	value					987:991	the value	983:991	the value of acorns	983:1001	The data presented here support the value of acorns as a source of nutritionally rich compounds thus justifying their use a good alternative to other nuts.
32822899	0	26	theme	Quercus	45:51	arg1	morphotypes					64:74	Quercus ilex acorn morphotypes	45:74	Quercus ilex acorn morphotypes	45:74	Phytochemical composition and variability in Quercus ilex acorn morphotypes as determined by NIRS and MS-based approaches.
32822899	1	27	theme	acorns	261:266	arg1	use					250:252	the direct use	239:252	the direct use of the acorns as nuts or as a source of consumable flour	239:309	Quercus acorns have been used as a staple food since archeological times; currently, there is a renewed interest in the direct use of the acorns as nuts or as a source of consumable flour.
32822899	1	28	from	interest	227:234	arg1	use					250:252	the direct use	239:252	the direct use of the acorns as nuts or as a source of consumable flour	239:309	Quercus acorns have been used as a staple food since archeological times; currently, there is a renewed interest in the direct use of the acorns as nuts or as a source of consumable flour.
32822899	2	29	theme	phytochemical	332:344	arg1	composition					346:356	phytochemical composition	332:356	phytochemical composition	332:356	In this regard, the phytochemical composition and variability of various morphotypes of acorns from Quercus ilex have been determined by using two platforms, near infrared spectroscopy (NIRS) and mass spectrometry (MS)-based metabolomics.
32822899	2	30	theme	infrared	475:482	arg1	NIRS					498:501	NIRS	498:501	NIRS	498:501	In this regard, the phytochemical composition and variability of various morphotypes of acorns from Quercus ilex have been determined by using two platforms, near infrared spectroscopy (NIRS) and mass spectrometry (MS)-based metabolomics.
32822899	2	30	theme	infrared	475:482	arg1	spectroscopy					484:495	infrared spectroscopy	475:495	infrared spectroscopy (NIRS)	475:502	In this regard, the phytochemical composition and variability of various morphotypes of acorns from Quercus ilex have been determined by using two platforms, near infrared spectroscopy (NIRS) and mass spectrometry (MS)-based metabolomics.
32822899	2	31	dep	composition	346:356	arg1	the					328:330	the	328:330	the	328:330	In this regard, the phytochemical composition and variability of various morphotypes of acorns from Quercus ilex have been determined by using two platforms, near infrared spectroscopy (NIRS) and mass spectrometry (MS)-based metabolomics.
32822899	0	32	theme	acorn	58:62	arg1	morphotypes					64:74	Quercus ilex acorn morphotypes	45:74	Quercus ilex acorn morphotypes	45:74	Phytochemical composition and variability in Quercus ilex acorn morphotypes as determined by NIRS and MS-based approaches.
32822899	5	33	theme	Principal	719:727	arg1	analysis					739:746	Principal component analysis	719:746	Principal component analysis (PCA)	719:752	Principal component analysis (PCA) discriminated the morphotypes; 50 compounds out of 192 identified had the highest load over the first two PCA components (explaining 67.2% variability) and can be potential markers of variability.
32822899	5	33	theme	Principal	719:727	arg1	PCA					749:751	PCA	749:751	PCA	749:751	Principal component analysis (PCA) discriminated the morphotypes; 50 compounds out of 192 identified had the highest load over the first two PCA components (explaining 67.2% variability) and can be potential markers of variability.
32822899	5	34	theme	potential	917:925	arg1	markers					927:933	potential markers	917:933	potential markers of variability	917:948	Principal component analysis (PCA) discriminated the morphotypes; 50 compounds out of 192 identified had the highest load over the first two PCA components (explaining 67.2% variability) and can be potential markers of variability.
32822899	4	35	theme	UHPLC-QToF	618:627	arg1	analysis					629:636	UHPLC-QToF analysis	618:636	UHPLC-QToF analysis	618:636	UHPLC-QToF analysis was used for untargeted metabolomic analysis and 192 metabolites were annotated.
32822899	0	36	theme	ilex	53:56	arg1	morphotypes					64:74	Quercus ilex acorn morphotypes	45:74	Quercus ilex acorn morphotypes	45:74	Phytochemical composition and variability in Quercus ilex acorn morphotypes as determined by NIRS and MS-based approaches.
32822899	2	37	theme	mass	508:511	arg1	MS					527:528	MS	527:528	MS	527:528	In this regard, the phytochemical composition and variability of various morphotypes of acorns from Quercus ilex have been determined by using two platforms, near infrared spectroscopy (NIRS) and mass spectrometry (MS)-based metabolomics.
32822899	2	37	theme	mass	508:511	arg1	spectrometry					513:524	mass spectrometry	508:524	mass spectrometry (MS)	508:529	In this regard, the phytochemical composition and variability of various morphotypes of acorns from Quercus ilex have been determined by using two platforms, near infrared spectroscopy (NIRS) and mass spectrometry (MS)-based metabolomics.
32822899	3	38	theme	abundant	590:597	arg1	%					614:614	50-60%	609:614	50-60%	609:614	According to NIRS, starch was the most abundant compound (50-60%).
32822899	3	38	theme	abundant	590:597	arg1	starch					570:575	starch	570:575	starch	570:575	According to NIRS, starch was the most abundant compound (50-60%).
32822899	3	38	theme	abundant	590:597	arg1	compound					599:606	the most abundant compound	581:606	the most abundant compound (50-60%)	581:615	According to NIRS, starch was the most abundant compound (50-60%).
32822899	1	39	theme	Quercus	123:129	arg1	acorns					131:136	Quercus acorns	123:136	Quercus acorns	123:136	Quercus acorns have been used as a staple food since archeological times; currently, there is a renewed interest in the direct use of the acorns as nuts or as a source of consumable flour.
32822899	1	39	theme	Quercus	123:129	arg1	food					165:168	a staple food	156:168	a staple food	156:168	Quercus acorns have been used as a staple food since archeological times; currently, there is a renewed interest in the direct use of the acorns as nuts or as a source of consumable flour.
32822899	5	40	theme	variability	938:948	arg1	markers					927:933	potential markers	917:933	potential markers of variability	917:948	Principal component analysis (PCA) discriminated the morphotypes; 50 compounds out of 192 identified had the highest load over the first two PCA components (explaining 67.2% variability) and can be potential markers of variability.
32822899	5	41	theme	%	891:891	arg1	variability					893:903	67.2% variability	887:903	67.2% variability	887:903	Principal component analysis (PCA) discriminated the morphotypes; 50 compounds out of 192 identified had the highest load over the first two PCA components (explaining 67.2% variability) and can be potential markers of variability.
32822899	5	42	dep	192	805:807	arg1	out					798:800	out	798:800	out	798:800	Principal component analysis (PCA) discriminated the morphotypes; 50 compounds out of 192 identified had the highest load over the first two PCA components (explaining 67.2% variability) and can be potential markers of variability.
32822899	6	43	theme	rich	1032:1035	arg1	compounds					1037:1045	nutritionally rich compounds	1018:1045	nutritionally rich compounds	1018:1045	The data presented here support the value of acorns as a source of nutritionally rich compounds thus justifying their use a good alternative to other nuts.
32822899	5	44	theme	67.2	887:890	arg1	%					891:891	%	891:891	%	891:891	Principal component analysis (PCA) discriminated the morphotypes; 50 compounds out of 192 identified had the highest load over the first two PCA components (explaining 67.2% variability) and can be potential markers of variability.
32822899	5	45	contain	had	820:822	arg2	load					836:839	the highest load	824:839	the highest load	824:839	Principal component analysis (PCA) discriminated the morphotypes; 50 compounds out of 192 identified had the highest load over the first two PCA components (explaining 67.2% variability) and can be potential markers of variability.
32822899	5	45	contain	had	820:822	arg1	compounds					788:796	50 compounds	785:796	50 compounds out of 192 identified	785:818	Principal component analysis (PCA) discriminated the morphotypes; 50 compounds out of 192 identified had the highest load over the first two PCA components (explaining 67.2% variability) and can be potential markers of variability.
32822899	5	46	theme	192	805:807	arg1	compounds					788:796	50 compounds	785:796	50 compounds out of 192 identified	785:818	Principal component analysis (PCA) discriminated the morphotypes; 50 compounds out of 192 identified had the highest load over the first two PCA components (explaining 67.2% variability) and can be potential markers of variability.
32822899	5	47	dep	components	864:873	arg1	explaining					876:885	explaining	876:885	explaining 67.2% variability	876:903	Principal component analysis (PCA) discriminated the morphotypes; 50 compounds out of 192 identified had the highest load over the first two PCA components (explaining 67.2% variability) and can be potential markers of variability.
32822899	6	48	theme	acorns	996:1001	arg1	source					1008:1013	a source	1006:1013	a source of nutritionally rich compounds	1006:1045	The data presented here support the value of acorns as a source of nutritionally rich compounds thus justifying their use a good alternative to other nuts.
32822899	6	48	theme	acorns	996:1001	arg1	value					987:991	the value	983:991	the value of acorns	983:1001	The data presented here support the value of acorns as a source of nutritionally rich compounds thus justifying their use a good alternative to other nuts.
32822899	2	49	theme	Quercus	412:418	arg1	ilex					420:423	Quercus ilex	412:423	Quercus ilex	412:423	In this regard, the phytochemical composition and variability of various morphotypes of acorns from Quercus ilex have been determined by using two platforms, near infrared spectroscopy (NIRS) and mass spectrometry (MS)-based metabolomics.
32822899	0	50	from	variability	30:40	arg1	morphotypes					64:74	Quercus ilex acorn morphotypes	45:74	Quercus ilex acorn morphotypes	45:74	Phytochemical composition and variability in Quercus ilex acorn morphotypes as determined by NIRS and MS-based approaches.
32822899	6	51	theme	good	1075:1078	arg1	alternative					1080:1090	a good alternative	1073:1090	a good alternative to other nuts	1073:1104	The data presented here support the value of acorns as a source of nutritionally rich compounds thus justifying their use a good alternative to other nuts.
32822899	5	52	theme	component	729:737	arg1	analysis					739:746	Principal component analysis	719:746	Principal component analysis (PCA)	719:752	Principal component analysis (PCA) discriminated the morphotypes; 50 compounds out of 192 identified had the highest load over the first two PCA components (explaining 67.2% variability) and can be potential markers of variability.
32822899	5	52	theme	component	729:737	arg1	PCA					749:751	PCA	749:751	PCA	749:751	Principal component analysis (PCA) discriminated the morphotypes; 50 compounds out of 192 identified had the highest load over the first two PCA components (explaining 67.2% variability) and can be potential markers of variability.
32822899	1	53	theme	renewed	219:225	arg1	interest					227:234	a renewed interest	217:234	a renewed interest in the direct use of the acorns as nuts or as a source of consumable flour	217:309	Quercus acorns have been used as a staple food since archeological times; currently, there is a renewed interest in the direct use of the acorns as nuts or as a source of consumable flour.
35011046	3	0	theme	n-3	559:561	arg1	PUFAs					563:567	n-3 PUFAs	559:567	n-3 PUFAs	559:567	We investigated the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice.
35011046	13	1	theme	n-3	1935:1937	arg1	PUFAs					1939:1943	scGOS:lcFOS and n-3 PUFAs	1919:1943	PUFAs	1939:1943	The results suggest that scGOS:lcFOS and n-3 PUFAs together create a balance-the best of both in a healthy host.
35011046	1	2	dep	composition	181:191	arg1	The					177:179	The	177:179	The	177:179	The composition and activity of the intestinal microbial community structures can be beneficially modulated by nutritional components such as non-digestible oligosaccharides and omega-3 poly-unsaturated fatty acids (n-3 PUFAs).
35011046	3	3	theme	community	597:605	arg1	structures					607:616	microbial community structures	587:616	microbial community structures	587:616	We investigated the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice.
35011046	7	4	theme	saccharolytic	1349:1361	arg1	activity					1376:1383	caecal saccharolytic fermentation activity	1342:1383	caecal saccharolytic fermentation activity	1342:1383	Compared to control diet, the scGOS:lcFOS and combination diets increased caecal saccharolytic fermentation activity.
35011046	5	5	from	Behaviour	916:924	arg1	MLN					1097:1099	MLN	1097:1099	MLN	1097:1099	Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed.
35011046	5	5	from	Behaviour	916:924	arg1	nodes					1090:1094	the mesenteric lymph nodes	1069:1094	the mesenteric lymph nodes (MLN)	1069:1100	Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed.
35011046	11	6	theme	limited	1755:1761	arg1	effects					1763:1769	limited effects	1755:1769	limited effects	1755:1769	The combination and individual interventions affected caecal content microbial profiles, but had limited effects on behaviour and the immune system.
35011046	1	7	theme	intestinal	213:222	arg1	structures					244:253	the intestinal microbial community structures	209:253	the intestinal microbial community structures	209:253	The composition and activity of the intestinal microbial community structures can be beneficially modulated by nutritional components such as non-digestible oligosaccharides and omega-3 poly-unsaturated fatty acids (n-3 PUFAs).
35011046	5	8	theme	brain	997:1001	arg1	levels					1013:1018	brain monoamine levels	997:1018	brain monoamine levels	997:1018	Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed.
35011046	1	9	theme	community	234:242	arg1	structures					244:253	the intestinal microbial community structures	209:253	the intestinal microbial community structures	209:253	The composition and activity of the intestinal microbial community structures can be beneficially modulated by nutritional components such as non-digestible oligosaccharides and omega-3 poly-unsaturated fatty acids (n-3 PUFAs).
35011046	4	10	theme	6	891:891	arg1	%					892:892	%	892:892	%	892:892	Male BALB/cByJ mice received a control or enriched diet with a combination of scGOS:lcFOS (9:1) and 6% tuna oil (n-3 PUFAs) or individually scGOS:lcFOS (9:1) or 6% tuna oil (n-3 PUFAs).
35011046	0	11	theme	System-Brain	158:169	arg1	Axis					171:174	the Gut-Immune System-Brain Axis	143:174	the Gut-Immune System-Brain Axis	143:174	Dietary Supplementation throughout Life with Non-Digestible Oligosaccharides and/or n-3 Poly-Unsaturated Fatty Acids in Healthy Mice Modulates the Gut-Immune System-Brain Axis.
35011046	5	12	from	levels	1013:1018	arg1	MLN					1097:1099	MLN	1097:1099	MLN	1097:1099	Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed.
35011046	5	12	from	levels	1013:1018	arg1	nodes					1090:1094	the mesenteric lymph nodes	1069:1094	the mesenteric lymph nodes (MLN)	1069:1100	Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed.
35011046	6	13	theme	community	1153:1161	arg1	structures					1163:1172	Caecal content microbial community structures	1128:1172	Caecal content microbial community structures	1128:1172	Caecal content microbial community structures displayed differences between the control and dietary groups, and between the dietary groups.
35011046	5	14	theme	fatty	978:982	arg1	acid					984:987	short-chain fatty acid	966:987	short-chain fatty acid levels	966:994	Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed.
35011046	3	15	theme	immune	641:646	arg1	system					648:653	the immune system	637:653	the immune system	637:653	We investigated the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice.
35011046	3	16	dep	content	579:585	arg1	development					622:632	development	622:632	development of the immune system	622:653	We investigated the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice.
35011046	3	16	dep	content	579:585	arg1	structures					607:616	microbial community structures	587:616	microbial community structures	587:616	We investigated the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice.
35011046	6	17	theme	content	1135:1141	arg1	structures					1163:1172	Caecal content microbial community structures	1128:1172	Caecal content microbial community structures	1128:1172	Caecal content microbial community structures displayed differences between the control and dietary groups, and between the dietary groups.
35011046	4	18	theme	control	761:767	arg1	diet					781:784	a control or enriched diet	759:784	a control or enriched diet with a combination of scGOS:lcFOS (9:1) and 6% tuna oil (n-3 PUFAs) or individually scGOS:lcFOS (9:1) or 6% tuna oil (n-3 PUFAs)	759:913	Male BALB/cByJ mice received a control or enriched diet with a combination of scGOS:lcFOS (9:1) and 6% tuna oil (n-3 PUFAs) or individually scGOS:lcFOS (9:1) or 6% tuna oil (n-3 PUFAs).
35011046	8	19	theme	enterochromaffin	1419:1434	arg1	cells					1436:1440	enterochromaffin cells	1419:1440	enterochromaffin cells	1419:1440	The diets enhanced the number of enterochromaffin cells.
35011046	7	20	theme	control	1280:1286	arg1	diet					1288:1291	control diet	1280:1291	control diet	1280:1291	Compared to control diet, the scGOS:lcFOS and combination diets increased caecal saccharolytic fermentation activity.
35011046	11	21	theme	individual	1678:1687	arg1	interventions					1689:1701	individual interventions	1678:1701	individual interventions	1678:1701	The combination and individual interventions affected caecal content microbial profiles, but had limited effects on behaviour and the immune system.
35011046	0	22	theme	n-3	84:86	arg1	Acids					111:115	n-3 Poly-Unsaturated Fatty Acids	84:115	n-3 Poly-Unsaturated Fatty Acids	84:115	Dietary Supplementation throughout Life with Non-Digestible Oligosaccharides and/or n-3 Poly-Unsaturated Fatty Acids in Healthy Mice Modulates the Gut-Immune System-Brain Axis.
35011046	10	23	theme	dietary	1513:1519	arg1	effect					1521:1526	the dietary effect	1509:1526	the dietary effect on behaviour	1509:1539	Although the dietary effect on behaviour was limited, serotonin and serotonin metabolite levels in the amygdala were increased in the combination diet group.
35011046	4	24	theme	BALB/cByJ	735:743	arg1	mice					745:748	Male BALB/cByJ mice	730:748	Male BALB/cByJ mice	730:748	Male BALB/cByJ mice received a control or enriched diet with a combination of scGOS:lcFOS (9:1) and 6% tuna oil (n-3 PUFAs) or individually scGOS:lcFOS (9:1) or 6% tuna oil (n-3 PUFAs).
35011046	0	25	theme	Fatty	105:109	arg1	Acids					111:115	n-3 Poly-Unsaturated Fatty Acids	84:115	n-3 Poly-Unsaturated Fatty Acids	84:115	Dietary Supplementation throughout Life with Non-Digestible Oligosaccharides and/or n-3 Poly-Unsaturated Fatty Acids in Healthy Mice Modulates the Gut-Immune System-Brain Axis.
35011046	13	26	theme	healthy	1993:1999	arg1	host					2001:2004	a healthy host	1991:2004	a healthy host	1991:2004	The results suggest that scGOS:lcFOS and n-3 PUFAs together create a balance-the best of both in a healthy host.
35011046	4	27	theme	tuna	894:897	arg1	oil					899:901	6% tuna oil	891:901	6% tuna oil	891:901	Male BALB/cByJ mice received a control or enriched diet with a combination of scGOS:lcFOS (9:1) and 6% tuna oil (n-3 PUFAs) or individually scGOS:lcFOS (9:1) or 6% tuna oil (n-3 PUFAs).
35011046	11	28	theme	content	1719:1725	arg1	profiles					1737:1744	caecal content microbial profiles	1712:1744	caecal content microbial profiles	1712:1744	The combination and individual interventions affected caecal content microbial profiles, but had limited effects on behaviour and the immune system.
35011046	5	29	theme	enterochromaffin	1021:1036	arg1	cells					1038:1042	enterochromaffin cells	1021:1042	enterochromaffin cells	1021:1042	Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed.
35011046	12	30	theme	n-3	1869:1871	arg1	PUFAs					1873:1877	n-3 PUFAs	1869:1877	n-3 PUFAs	1869:1877	No apparent additive effect was observed when scGOS:lcFOS and n-3 PUFAs were combined.
35011046	3	31	dep	scGOS	543:547	arg1	lcFOS					549:553	lcFOS	549:553	lcFOS	549:553	We investigated the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice.
35011046	3	31	dep	scGOS	543:547	arg1	PUFAs					563:567	n-3 PUFAs	559:567	n-3 PUFAs	559:567	We investigated the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice.
35011046	0	32	from	Oligosaccharides	60:75	arg1	Mice					128:131	Healthy Mice	120:131	Healthy Mice	120:131	Dietary Supplementation throughout Life with Non-Digestible Oligosaccharides and/or n-3 Poly-Unsaturated Fatty Acids in Healthy Mice Modulates the Gut-Immune System-Brain Axis.
35011046	4	33	theme	scGOS	808:812	arg1	combination					793:803	a combination	791:803	a combination of scGOS:lcFOS (9:1) and 6% tuna oil (n-3 PUFAs) or individually scGOS:lcFOS (9:1) or 6% tuna oil (n-3 PUFAs)	791:913	Male BALB/cByJ mice received a control or enriched diet with a combination of scGOS:lcFOS (9:1) and 6% tuna oil (n-3 PUFAs) or individually scGOS:lcFOS (9:1) or 6% tuna oil (n-3 PUFAs).
35011046	3	34	theme	additive	499:506	arg1	effect					508:513	the additive effect	495:513	the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice	495:727	We investigated the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice.
35011046	11	35	theme	caecal	1712:1717	arg1	profiles					1737:1744	caecal content microbial profiles	1712:1744	caecal content microbial profiles	1712:1744	The combination and individual interventions affected caecal content microbial profiles, but had limited effects on behaviour and the immune system.
35011046	0	36	theme	Poly-Unsaturated	88:103	arg1	Acids					111:115	n-3 Poly-Unsaturated Fatty Acids	84:115	n-3 Poly-Unsaturated Fatty Acids	84:115	Dietary Supplementation throughout Life with Non-Digestible Oligosaccharides and/or n-3 Poly-Unsaturated Fatty Acids in Healthy Mice Modulates the Gut-Immune System-Brain Axis.
35011046	4	37	theme	enriched	772:779	arg1	diet					781:784	a control or enriched diet	759:784	a control or enriched diet with a combination of scGOS:lcFOS (9:1) and 6% tuna oil (n-3 PUFAs) or individually scGOS:lcFOS (9:1) or 6% tuna oil (n-3 PUFAs)	759:913	Male BALB/cByJ mice received a control or enriched diet with a combination of scGOS:lcFOS (9:1) and 6% tuna oil (n-3 PUFAs) or individually scGOS:lcFOS (9:1) or 6% tuna oil (n-3 PUFAs).
35011046	0	38	theme	Dietary	0:6	arg1	Supplementation					8:22	Dietary Supplementation	0:22	Dietary Supplementation throughout Life with Non-Digestible Oligosaccharides and/or n-3 Poly-Unsaturated Fatty Acids in Healthy Mice	0:131	Dietary Supplementation throughout Life with Non-Digestible Oligosaccharides and/or n-3 Poly-Unsaturated Fatty Acids in Healthy Mice Modulates the Gut-Immune System-Brain Axis.
35011046	1	39	theme	non-digestible	319:332	arg1	oligosaccharides					334:349	non-digestible oligosaccharides	319:349	non-digestible oligosaccharides	319:349	The composition and activity of the intestinal microbial community structures can be beneficially modulated by nutritional components such as non-digestible oligosaccharides and omega-3 poly-unsaturated fatty acids (n-3 PUFAs).
35011046	6	40	theme	control	1208:1214	arg1	groups					1228:1233	the control and dietary groups	1204:1233	groups	1228:1233	Caecal content microbial community structures displayed differences between the control and dietary groups, and between the dietary groups.
35011046	3	41	theme	dietary	520:526	arg1	combination					528:538	a dietary combination	518:538	a dietary combination of scGOS:lcFOS and n-3 PUFAs	518:567	We investigated the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice.
35011046	5	42	from	cells	1038:1042	arg1	MLN					1097:1099	MLN	1097:1099	MLN	1097:1099	Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed.
35011046	5	42	from	cells	1038:1042	arg1	nodes					1090:1094	the mesenteric lymph nodes	1069:1094	the mesenteric lymph nodes (MLN)	1069:1100	Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed.
35011046	12	43	theme	apparent	1810:1817	arg1	effect					1828:1833	No apparent additive effect	1807:1833	No apparent additive effect	1807:1833	No apparent additive effect was observed when scGOS:lcFOS and n-3 PUFAs were combined.
35011046	5	44	theme	lymph	1084:1088	arg1	MLN					1097:1099	MLN	1097:1099	MLN	1097:1099	Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed.
35011046	5	44	theme	lymph	1084:1088	arg1	nodes					1090:1094	the mesenteric lymph nodes	1069:1094	the mesenteric lymph nodes (MLN)	1069:1100	Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed.
35011046	10	45	from	levels	1589:1594	arg1	amygdala					1603:1610	the amygdala	1599:1610	the amygdala	1599:1610	Although the dietary effect on behaviour was limited, serotonin and serotonin metabolite levels in the amygdala were increased in the combination diet group.
35011046	1	46	theme	poly-unsaturated	363:378	arg1	acids					386:390	omega-3 poly-unsaturated fatty acids	355:390	omega-3 poly-unsaturated fatty acids (n-3 PUFAs)	355:402	The composition and activity of the intestinal microbial community structures can be beneficially modulated by nutritional components such as non-digestible oligosaccharides and omega-3 poly-unsaturated fatty acids (n-3 PUFAs).
35011046	1	46	theme	poly-unsaturated	363:378	arg1	PUFAs					397:401	n-3 PUFAs	393:401	n-3 PUFAs	393:401	The composition and activity of the intestinal microbial community structures can be beneficially modulated by nutritional components such as non-digestible oligosaccharides and omega-3 poly-unsaturated fatty acids (n-3 PUFAs).
35011046	4	47	theme	%	831:831	arg1	oil					838:840	6% tuna oil	830:840	6% tuna oil	830:840	Male BALB/cByJ mice received a control or enriched diet with a combination of scGOS:lcFOS (9:1) and 6% tuna oil (n-3 PUFAs) or individually scGOS:lcFOS (9:1) or 6% tuna oil (n-3 PUFAs).
35011046	5	48	theme	short-chain	966:976	arg1	acid					984:987	short-chain fatty acid	966:987	short-chain fatty acid levels	966:994	Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed.
35011046	3	49	theme	healthy	716:722	arg1	mice					724:727	healthy mice	716:727	healthy mice	716:727	We investigated the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice.
35011046	10	50	theme	combination	1634:1644	arg1	group					1651:1655	the combination diet group	1630:1655	the combination diet group	1630:1655	Although the dietary effect on behaviour was limited, serotonin and serotonin metabolite levels in the amygdala were increased in the combination diet group.
35011046	10	51	from	effect	1521:1526	arg1	behaviour					1531:1539	behaviour	1531:1539	behaviour	1531:1539	Although the dietary effect on behaviour was limited, serotonin and serotonin metabolite levels in the amygdala were increased in the combination diet group.
35011046	11	52	contain	had	1751:1753	arg2	effects					1763:1769	limited effects	1755:1769	limited effects	1755:1769	The combination and individual interventions affected caecal content microbial profiles, but had limited effects on behaviour and the immune system.
35011046	11	52	contain	had	1751:1753	arg1	combination					1662:1672	The combination	1658:1672	The combination	1658:1672	The combination and individual interventions affected caecal content microbial profiles, but had limited effects on behaviour and the immune system.
35011046	2	53	theme	immune	429:434	arg1	function					436:443	immune function	429:443	immune function	429:443	These components affect immune function, brain development and behaviour.
35011046	5	54	theme	content	934:940	arg1	composition					953:963	caecal content microbiota composition	927:963	caecal content microbiota composition	927:963	Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed.
35011046	3	55	from	effect	508:513	arg1	behaviour					666:674	behaviour	666:674	behaviour	666:674	We investigated the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice.
35011046	3	55	from	effect	508:513	arg1	content					579:585	caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice	572:727	content	579:585	We investigated the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice.
35011046	3	55	from	effect	508:513	arg1	brain					656:660	brain	656:660	brain	656:660	We investigated the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice.
35011046	1	56	theme	nutritional	288:298	arg1	components					300:309	nutritional components	288:309	nutritional components such as non-digestible oligosaccharides and omega-3 poly-unsaturated fatty acids (n-3 PUFAs)	288:402	The composition and activity of the intestinal microbial community structures can be beneficially modulated by nutritional components such as non-digestible oligosaccharides and omega-3 poly-unsaturated fatty acids (n-3 PUFAs).
35011046	1	56	theme	nutritional	288:298	arg1	acids					386:390	omega-3 poly-unsaturated fatty acids	355:390	omega-3 poly-unsaturated fatty acids (n-3 PUFAs)	355:402	The composition and activity of the intestinal microbial community structures can be beneficially modulated by nutritional components such as non-digestible oligosaccharides and omega-3 poly-unsaturated fatty acids (n-3 PUFAs).
35011046	1	56	theme	nutritional	288:298	arg1	oligosaccharides					334:349	non-digestible oligosaccharides	319:349	non-digestible oligosaccharides	319:349	The composition and activity of the intestinal microbial community structures can be beneficially modulated by nutritional components such as non-digestible oligosaccharides and omega-3 poly-unsaturated fatty acids (n-3 PUFAs).
35011046	3	57	from	day	681:683	arg1	behaviour					666:674	behaviour	666:674	behaviour	666:674	We investigated the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice.
35011046	3	57	from	day	681:683	arg1	content					579:585	caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice	572:727	content	579:585	We investigated the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice.
35011046	3	57	from	day	681:683	arg1	brain					656:660	brain	656:660	brain	656:660	We investigated the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice.
35011046	6	58	theme	dietary	1220:1226	arg1	groups					1228:1233	the control and dietary groups	1204:1233	groups	1228:1233	Caecal content microbial community structures displayed differences between the control and dietary groups, and between the dietary groups.
35011046	3	59	theme	microbial	587:595	arg1	structures					607:616	microbial community structures	587:616	microbial community structures	587:616	We investigated the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice.
35011046	5	60	from	spleen	1106:1111	arg1	MLN					1097:1099	MLN	1097:1099	MLN	1097:1099	Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed.
35011046	5	60	from	spleen	1106:1111	arg1	nodes					1090:1094	the mesenteric lymph nodes	1069:1094	the mesenteric lymph nodes (MLN)	1069:1100	Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed.
35011046	13	61	theme	scGOS	1919:1923	arg1	lcFOS					1925:1929	scGOS:lcFOS and n-3 PUFAs	1919:1943	lcFOS	1925:1929	The results suggest that scGOS:lcFOS and n-3 PUFAs together create a balance-the best of both in a healthy host.
35011046	3	62	theme	caecal	572:577	arg1	behaviour					666:674	behaviour	666:674	behaviour	666:674	We investigated the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice.
35011046	3	62	theme	caecal	572:577	arg1	content					579:585	caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice	572:727	content	579:585	We investigated the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice.
35011046	3	62	theme	caecal	572:577	arg1	brain					656:660	brain	656:660	brain	656:660	We investigated the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice.
35011046	7	63	dep	scGOS	1298:1302	arg1	increased					1332:1340	increased	1332:1340	increased caecal saccharolytic fermentation activity	1332:1383	Compared to control diet, the scGOS:lcFOS and combination diets increased caecal saccharolytic fermentation activity.
35011046	4	64	theme	tuna	833:836	arg1	oil					838:840	6% tuna oil	830:840	6% tuna oil	830:840	Male BALB/cByJ mice received a control or enriched diet with a combination of scGOS:lcFOS (9:1) and 6% tuna oil (n-3 PUFAs) or individually scGOS:lcFOS (9:1) or 6% tuna oil (n-3 PUFAs).
35011046	7	65	theme	fermentation	1363:1374	arg1	activity					1376:1383	caecal saccharolytic fermentation activity	1342:1383	caecal saccharolytic fermentation activity	1342:1383	Compared to control diet, the scGOS:lcFOS and combination diets increased caecal saccharolytic fermentation activity.
35011046	7	66	dep	lcFOS	1304:1308	arg1	diets					1326:1330	diets	1326:1330	diets	1326:1330	Compared to control diet, the scGOS:lcFOS and combination diets increased caecal saccharolytic fermentation activity.
35011046	7	67	theme	caecal	1342:1347	arg1	activity					1376:1383	caecal saccharolytic fermentation activity	1342:1383	caecal saccharolytic fermentation activity	1342:1383	Compared to control diet, the scGOS:lcFOS and combination diets increased caecal saccharolytic fermentation activity.
35011046	0	68	theme	Gut-Immune	147:156	arg1	Axis					171:174	the Gut-Immune System-Brain Axis	143:174	the Gut-Immune System-Brain Axis	143:174	Dietary Supplementation throughout Life with Non-Digestible Oligosaccharides and/or n-3 Poly-Unsaturated Fatty Acids in Healthy Mice Modulates the Gut-Immune System-Brain Axis.
35011046	1	69	theme	microbial	224:232	arg1	structures					244:253	the intestinal microbial community structures	209:253	the intestinal microbial community structures	209:253	The composition and activity of the intestinal microbial community structures can be beneficially modulated by nutritional components such as non-digestible oligosaccharides and omega-3 poly-unsaturated fatty acids (n-3 PUFAs).
35011046	10	70	theme	serotonin	1568:1576	arg1	levels					1589:1594	serotonin and serotonin metabolite levels	1554:1594	serotonin and serotonin metabolite levels in the amygdala	1554:1610	Although the dietary effect on behaviour was limited, serotonin and serotonin metabolite levels in the amygdala were increased in the combination diet group.
35011046	5	71	theme	monoamine	1003:1011	arg1	levels					1013:1018	brain monoamine levels	997:1018	brain monoamine levels	997:1018	Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed.
35011046	3	72	theme	system	648:653	arg1	development					622:632	development	622:632	development of the immune system	622:653	We investigated the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice.
35011046	3	72	theme	system	648:653	arg1	structures					607:616	microbial community structures	587:616	microbial community structures	587:616	We investigated the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice.
35011046	5	73	from	composition	953:963	arg1	MLN					1097:1099	MLN	1097:1099	MLN	1097:1099	Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed.
35011046	5	73	from	composition	953:963	arg1	nodes					1090:1094	the mesenteric lymph nodes	1069:1094	the mesenteric lymph nodes (MLN)	1069:1100	Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed.
35011046	1	74	theme	structures	244:253	arg1	activity					197:204	activity	197:204	activity	197:204	The composition and activity of the intestinal microbial community structures can be beneficially modulated by nutritional components such as non-digestible oligosaccharides and omega-3 poly-unsaturated fatty acids (n-3 PUFAs).
35011046	1	74	theme	structures	244:253	arg1	composition					181:191	composition	181:191	composition	181:191	The composition and activity of the intestinal microbial community structures can be beneficially modulated by nutritional components such as non-digestible oligosaccharides and omega-3 poly-unsaturated fatty acids (n-3 PUFAs).
35011046	10	75	theme	serotonin	1554:1562	arg1	levels					1589:1594	serotonin and serotonin metabolite levels	1554:1594	serotonin and serotonin metabolite levels in the amygdala	1554:1610	Although the dietary effect on behaviour was limited, serotonin and serotonin metabolite levels in the amygdala were increased in the combination diet group.
35011046	4	76	with	diet	781:784	arg1	combination					793:803	a combination	791:803	a combination of scGOS:lcFOS (9:1) and 6% tuna oil (n-3 PUFAs) or individually scGOS:lcFOS (9:1) or 6% tuna oil (n-3 PUFAs)	791:913	Male BALB/cByJ mice received a control or enriched diet with a combination of scGOS:lcFOS (9:1) and 6% tuna oil (n-3 PUFAs) or individually scGOS:lcFOS (9:1) or 6% tuna oil (n-3 PUFAs).
35011046	6	77	theme	dietary	1252:1258	arg1	groups					1260:1265	the dietary groups	1248:1265	the dietary groups	1248:1265	Caecal content microbial community structures displayed differences between the control and dietary groups, and between the dietary groups.
35011046	6	78	theme	microbial	1143:1151	arg1	structures					1163:1172	Caecal content microbial community structures	1128:1172	Caecal content microbial community structures	1128:1172	Caecal content microbial community structures displayed differences between the control and dietary groups, and between the dietary groups.
35011046	5	79	theme	acid	984:987	arg1	levels					989:994	short-chain fatty acid levels	966:994	short-chain fatty acid levels	966:994	Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed.
35011046	9	80	theme	combination	1447:1457	arg1	diet					1459:1462	The combination diet	1443:1462	The combination diet	1443:1462	The combination diet had no effects on the immune cells.
35011046	8	81	theme	cells	1436:1440	arg1	number					1409:1414	the number	1405:1414	the number of enterochromaffin cells	1405:1440	The diets enhanced the number of enterochromaffin cells.
35011046	6	82	theme	Caecal	1128:1133	arg1	structures					1163:1172	Caecal content microbial community structures	1128:1172	Caecal content microbial community structures	1128:1172	Caecal content microbial community structures displayed differences between the control and dietary groups, and between the dietary groups.
35011046	11	83	theme	immune	1792:1797	arg1	system					1799:1804	the immune system	1788:1804	the immune system	1788:1804	The combination and individual interventions affected caecal content microbial profiles, but had limited effects on behaviour and the immune system.
35011046	5	84	from	levels	989:994	arg1	MLN					1097:1099	MLN	1097:1099	MLN	1097:1099	Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed.
35011046	5	84	from	levels	989:994	arg1	nodes					1090:1094	the mesenteric lymph nodes	1069:1094	the mesenteric lymph nodes (MLN)	1069:1100	Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed.
35011046	4	85	dep	scGOS	808:812	arg1	9:1					821:823	9:1	821:823	9:1	821:823	Male BALB/cByJ mice received a control or enriched diet with a combination of scGOS:lcFOS (9:1) and 6% tuna oil (n-3 PUFAs) or individually scGOS:lcFOS (9:1) or 6% tuna oil (n-3 PUFAs).
35011046	4	85	dep	scGOS	808:812	arg1	oil					838:840	6% tuna oil	830:840	6% tuna oil	830:840	Male BALB/cByJ mice received a control or enriched diet with a combination of scGOS:lcFOS (9:1) and 6% tuna oil (n-3 PUFAs) or individually scGOS:lcFOS (9:1) or 6% tuna oil (n-3 PUFAs).
35011046	4	85	dep	scGOS	808:812	arg1	PUFAs					908:912	n-3 PUFAs	904:912	n-3 PUFAs	904:912	Male BALB/cByJ mice received a control or enriched diet with a combination of scGOS:lcFOS (9:1) and 6% tuna oil (n-3 PUFAs) or individually scGOS:lcFOS (9:1) or 6% tuna oil (n-3 PUFAs).
35011046	4	85	dep	scGOS	808:812	arg1	scGOS					870:874	scGOS	870:874	scGOS	870:874	Male BALB/cByJ mice received a control or enriched diet with a combination of scGOS:lcFOS (9:1) and 6% tuna oil (n-3 PUFAs) or individually scGOS:lcFOS (9:1) or 6% tuna oil (n-3 PUFAs).
35011046	4	85	dep	scGOS	808:812	arg1	lcFOS					876:880	lcFOS	876:880	lcFOS (9:1)	876:886	Male BALB/cByJ mice received a control or enriched diet with a combination of scGOS:lcFOS (9:1) and 6% tuna oil (n-3 PUFAs) or individually scGOS:lcFOS (9:1) or 6% tuna oil (n-3 PUFAs).
35011046	4	85	dep	scGOS	808:812	arg1	PUFAs					847:851	n-3 PUFAs	843:851	n-3 PUFAs	843:851	Male BALB/cByJ mice received a control or enriched diet with a combination of scGOS:lcFOS (9:1) and 6% tuna oil (n-3 PUFAs) or individually scGOS:lcFOS (9:1) or 6% tuna oil (n-3 PUFAs).
35011046	4	85	dep	scGOS	808:812	arg1	oil					899:901	6% tuna oil	891:901	6% tuna oil	891:901	Male BALB/cByJ mice received a control or enriched diet with a combination of scGOS:lcFOS (9:1) and 6% tuna oil (n-3 PUFAs) or individually scGOS:lcFOS (9:1) or 6% tuna oil (n-3 PUFAs).
35011046	4	85	dep	scGOS	808:812	arg1	lcFOS					814:818	lcFOS	814:818	lcFOS (9:1)	814:824	Male BALB/cByJ mice received a control or enriched diet with a combination of scGOS:lcFOS (9:1) and 6% tuna oil (n-3 PUFAs) or individually scGOS:lcFOS (9:1) or 6% tuna oil (n-3 PUFAs).
35011046	4	85	dep	scGOS	808:812	arg1	9:1					883:885	9:1	883:885	9:1	883:885	Male BALB/cByJ mice received a control or enriched diet with a combination of scGOS:lcFOS (9:1) and 6% tuna oil (n-3 PUFAs) or individually scGOS:lcFOS (9:1) or 6% tuna oil (n-3 PUFAs).
35011046	4	86	theme	Male	730:733	arg1	mice					745:748	Male BALB/cByJ mice	730:748	Male BALB/cByJ mice	730:748	Male BALB/cByJ mice received a control or enriched diet with a combination of scGOS:lcFOS (9:1) and 6% tuna oil (n-3 PUFAs) or individually scGOS:lcFOS (9:1) or 6% tuna oil (n-3 PUFAs).
35011046	0	87	theme	Healthy	120:126	arg1	Mice					128:131	Healthy Mice	120:131	Healthy Mice	120:131	Dietary Supplementation throughout Life with Non-Digestible Oligosaccharides and/or n-3 Poly-Unsaturated Fatty Acids in Healthy Mice Modulates the Gut-Immune System-Brain Axis.
35011046	5	88	theme	mesenteric	1073:1082	arg1	MLN					1097:1099	MLN	1097:1099	MLN	1097:1099	Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed.
35011046	5	88	theme	mesenteric	1073:1082	arg1	nodes					1090:1094	the mesenteric lymph nodes	1069:1094	the mesenteric lymph nodes (MLN)	1069:1100	Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed.
35011046	4	89	theme	%	892:892	arg1	oil					899:901	6% tuna oil	891:901	6% tuna oil	891:901	Male BALB/cByJ mice received a control or enriched diet with a combination of scGOS:lcFOS (9:1) and 6% tuna oil (n-3 PUFAs) or individually scGOS:lcFOS (9:1) or 6% tuna oil (n-3 PUFAs).
35011046	9	90	theme	immune	1486:1491	arg1	cells					1493:1497	the immune cells	1482:1497	the immune cells	1482:1497	The combination diet had no effects on the immune cells.
35011046	11	91	theme	microbial	1727:1735	arg1	profiles					1737:1744	caecal content microbial profiles	1712:1744	caecal content microbial profiles	1712:1744	The combination and individual interventions affected caecal content microbial profiles, but had limited effects on behaviour and the immune system.
35011046	2	92	theme	brain	446:450	arg1	development					452:462	brain development	446:462	brain development	446:462	These components affect immune function, brain development and behaviour.
35011046	5	93	theme	immune	1048:1053	arg1	parameters					1055:1064	immune parameters	1048:1064	immune parameters	1048:1064	Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed.
35011046	10	94	theme	metabolite	1578:1587	arg1	levels					1589:1594	serotonin and serotonin metabolite levels	1554:1594	serotonin and serotonin metabolite levels in the amygdala	1554:1610	Although the dietary effect on behaviour was limited, serotonin and serotonin metabolite levels in the amygdala were increased in the combination diet group.
35011046	12	95	theme	additive	1819:1826	arg1	effect					1828:1833	No apparent additive effect	1807:1833	No apparent additive effect	1807:1833	No apparent additive effect was observed when scGOS:lcFOS and n-3 PUFAs were combined.
35011046	3	96	theme	birth	688:692	arg1	day					681:683	day	681:683	day of birth to early adulthood in healthy mice	681:727	We investigated the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice.
35011046	3	97	theme	combination	528:538	arg1	effect					508:513	the additive effect	495:513	the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice	495:727	We investigated the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice.
35011046	0	98	theme	Non-Digestible	45:58	arg1	Oligosaccharides					60:75	Non-Digestible Oligosaccharides	45:75	Non-Digestible Oligosaccharides	45:75	Dietary Supplementation throughout Life with Non-Digestible Oligosaccharides and/or n-3 Poly-Unsaturated Fatty Acids in Healthy Mice Modulates the Gut-Immune System-Brain Axis.
35011046	5	99	theme	microbiota	942:951	arg1	composition					953:963	caecal content microbiota composition	927:963	caecal content microbiota composition	927:963	Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed.
35011046	5	100	from	parameters	1055:1064	arg1	MLN					1097:1099	MLN	1097:1099	MLN	1097:1099	Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed.
35011046	5	100	from	parameters	1055:1064	arg1	nodes					1090:1094	the mesenteric lymph nodes	1069:1094	the mesenteric lymph nodes (MLN)	1069:1100	Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed.
35011046	1	101	theme	fatty	380:384	arg1	acids					386:390	omega-3 poly-unsaturated fatty acids	355:390	omega-3 poly-unsaturated fatty acids (n-3 PUFAs)	355:402	The composition and activity of the intestinal microbial community structures can be beneficially modulated by nutritional components such as non-digestible oligosaccharides and omega-3 poly-unsaturated fatty acids (n-3 PUFAs).
35011046	1	101	theme	fatty	380:384	arg1	PUFAs					397:401	n-3 PUFAs	393:401	n-3 PUFAs	393:401	The composition and activity of the intestinal microbial community structures can be beneficially modulated by nutritional components such as non-digestible oligosaccharides and omega-3 poly-unsaturated fatty acids (n-3 PUFAs).
35011046	4	102	theme	6	830:830	arg1	%					831:831	%	831:831	%	831:831	Male BALB/cByJ mice received a control or enriched diet with a combination of scGOS:lcFOS (9:1) and 6% tuna oil (n-3 PUFAs) or individually scGOS:lcFOS (9:1) or 6% tuna oil (n-3 PUFAs).
35011046	0	103	with	Supplementation	8:22	arg1	Acids					111:115	n-3 Poly-Unsaturated Fatty Acids	84:115	n-3 Poly-Unsaturated Fatty Acids	84:115	Dietary Supplementation throughout Life with Non-Digestible Oligosaccharides and/or n-3 Poly-Unsaturated Fatty Acids in Healthy Mice Modulates the Gut-Immune System-Brain Axis.
35011046	0	103	with	Supplementation	8:22	arg1	Oligosaccharides					60:75	Non-Digestible Oligosaccharides	45:75	Non-Digestible Oligosaccharides	45:75	Dietary Supplementation throughout Life with Non-Digestible Oligosaccharides and/or n-3 Poly-Unsaturated Fatty Acids in Healthy Mice Modulates the Gut-Immune System-Brain Axis.
35011046	10	104	theme	diet	1646:1649	arg1	group					1651:1655	the combination diet group	1630:1655	the combination diet group	1630:1655	Although the dietary effect on behaviour was limited, serotonin and serotonin metabolite levels in the amygdala were increased in the combination diet group.
35011046	9	105	contain	had	1464:1466	arg1	diet					1459:1462	The combination diet	1443:1462	The combination diet	1443:1462	The combination diet had no effects on the immune cells.
35011046	9	105	contain	had	1464:1466	arg2	effects					1471:1477	no effects	1468:1477	no effects	1468:1477	The combination diet had no effects on the immune cells.
35011046	3	106	theme	early	697:701	arg1	adulthood					703:711	early adulthood	697:711	early adulthood in healthy mice	697:727	We investigated the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice.
35011046	3	107	theme	scGOS	543:547	arg1	combination					528:538	a dietary combination	518:538	a dietary combination of scGOS:lcFOS and n-3 PUFAs	518:567	We investigated the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice.
35011046	5	108	theme	caecal	927:932	arg1	composition					953:963	caecal content microbiota composition	927:963	caecal content microbiota composition	927:963	Behaviour, caecal content microbiota composition, short-chain fatty acid levels, brain monoamine levels, enterochromaffin cells and immune parameters in the mesenteric lymph nodes (MLN) and spleen were assessed.
35011046	3	109	from	adulthood	703:711	arg1	mice					724:727	healthy mice	716:727	healthy mice	716:727	We investigated the additive effect of a dietary combination of scGOS:lcFOS and n-3 PUFAs on caecal content microbial community structures and development of the immune system, brain and behaviour from day of birth to early adulthood in healthy mice.
32235573	7	0	theme	-NH2	1275:1278	arg1	-OH					1270:1272	-OH	1270:1272	-OH	1270:1272	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	7	0	theme	-NH2	1275:1278	arg1	groups					1260:1265	the active functional groups	1238:1265	the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles	1238:1319	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	7	0	theme	-NH2	1275:1278	arg1	Na+					1230:1232	Na+	1230:1232	Na+	1230:1232	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	7	0	theme	-NH2	1275:1278	arg1	-COO-					1284:1288	-COO-	1284:1288	-COO-	1284:1288	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	7	0	theme	-NH2	1275:1278	arg1	-NH2					1275:1278	-NH2	1275:1278	-NH2	1275:1278	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	3	1	theme	influent	569:576	arg1	concentration					585:597	influent NH4+-N concentration	569:597	influent NH4+-N concentration (3, 5 and 7 mg/L)	569:615	Effect of bed depth (30, 50 and 70 cm), flow rate (32, 49 and 65 mL/min), initial pH value (4.5, 6.5 and 8.5) and influent NH4+-N concentration (3, 5 and 7 mg/L) was examined by using a filter column packed with CTS-ZMS particles.
32235573	3	1	theme	influent	569:576	arg1	mg/L					611:614	3, 5 and 7 mg/L	600:614	3, 5 and 7 mg/L	600:614	Effect of bed depth (30, 50 and 70 cm), flow rate (32, 49 and 65 mL/min), initial pH value (4.5, 6.5 and 8.5) and influent NH4+-N concentration (3, 5 and 7 mg/L) was examined by using a filter column packed with CTS-ZMS particles.
32235573	2	2	theme	zeolite	342:348	arg1	CTS-ZMS					367:373	CTS-ZMS	367:373	CTS-ZMS	367:373	In this research, a composite adsorbent based on chitosan crosslink with zeolite molecular sieve (CTS-ZMS) was prepared for NH4+-N removal through dynamic adsorption filter experiments.
32235573	2	2	theme	zeolite	342:348	arg1	sieve					360:364	zeolite molecular sieve	342:364	zeolite molecular sieve (CTS-ZMS)	342:374	In this research, a composite adsorbent based on chitosan crosslink with zeolite molecular sieve (CTS-ZMS) was prepared for NH4+-N removal through dynamic adsorption filter experiments.
32235573	7	3	theme	-OH	1270:1272	arg1	-OH					1270:1272	-OH	1270:1272	-OH	1270:1272	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	7	3	theme	-OH	1270:1272	arg1	groups					1260:1265	the active functional groups	1238:1265	the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles	1238:1319	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	7	3	theme	-OH	1270:1272	arg1	Na+					1230:1232	Na+	1230:1232	Na+	1230:1232	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	7	3	theme	-OH	1270:1272	arg1	-COO-					1284:1288	-COO-	1284:1288	-COO-	1284:1288	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	7	3	theme	-OH	1270:1272	arg1	-NH2					1275:1278	-NH2	1275:1278	-NH2	1275:1278	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	4	4	theme	breakthrough	728:739	arg1	curves					741:746	the breakthrough curves	724:746	the breakthrough curves	724:746	The Thomas model was applied to study the breakthrough curves and adsorption capacity.
32235573	6	5	theme	dispersive	992:1001	arg1	EDS					1023:1025	EDS	1023:1025	EDS	1023:1025	Scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and Fourier Transform Infrared Spectroscopy (FTIR) were investigated to analyze the structure and morphology of the CTS-ZMS adsorbents before and after 3 months running.
32235573	6	5	theme	dispersive	992:1001	arg1	spectroscopy					1009:1020	energy dispersive X-ray spectroscopy	985:1020	energy dispersive X-ray spectroscopy (EDS)	985:1026	Scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and Fourier Transform Infrared Spectroscopy (FTIR) were investigated to analyze the structure and morphology of the CTS-ZMS adsorbents before and after 3 months running.
32235573	8	6	theme	drinking	1419:1426	arg1	processes					1445:1453	drinking water filtration processes	1419:1453	drinking water filtration processes	1419:1453	The results of this study supported the use of CTS-ZMS to improve drinking water filtration processes by increasing ammonium nitrogen reductions.
32235573	3	7	theme	rate	500:503	arg1	Effect					455:460	Effect	455:460	Effect of bed depth (30, 50 and 70 cm), flow rate (32, 49 and 65 mL/min), initial pH value (4.5, 6.5 and 8.5) and influent NH4+-N concentration (3, 5 and 7 mg/L)	455:615	Effect of bed depth (30, 50 and 70 cm), flow rate (32, 49 and 65 mL/min), initial pH value (4.5, 6.5 and 8.5) and influent NH4+-N concentration (3, 5 and 7 mg/L) was examined by using a filter column packed with CTS-ZMS particles.
32235573	7	8	theme	active	1242:1247	arg1	-OH					1270:1272	-OH	1270:1272	-OH	1270:1272	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	7	8	theme	active	1242:1247	arg1	groups					1260:1265	the active functional groups	1238:1265	the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles	1238:1319	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	7	8	theme	active	1242:1247	arg1	-COO-					1284:1288	-COO-	1284:1288	-COO-	1284:1288	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	7	8	theme	active	1242:1247	arg1	-NH2					1275:1278	-NH2	1275:1278	-NH2	1275:1278	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	6	9	theme	Infrared	1050:1057	arg1	FTIR					1073:1076	FTIR	1073:1076	FTIR	1073:1076	Scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and Fourier Transform Infrared Spectroscopy (FTIR) were investigated to analyze the structure and morphology of the CTS-ZMS adsorbents before and after 3 months running.
32235573	6	9	theme	Infrared	1050:1057	arg1	Spectroscopy					1059:1070	Infrared Spectroscopy	1050:1070	Infrared Spectroscopy (FTIR)	1050:1077	Scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and Fourier Transform Infrared Spectroscopy (FTIR) were investigated to analyze the structure and morphology of the CTS-ZMS adsorbents before and after 3 months running.
32235573	2	10	theme	adsorbent	299:307	arg1	based					309:313	a composite adsorbent based	287:313	a composite adsorbent based on chitosan crosslink with zeolite molecular sieve (CTS-ZMS)	287:374	In this research, a composite adsorbent based on chitosan crosslink with zeolite molecular sieve (CTS-ZMS) was prepared for NH4+-N removal through dynamic adsorption filter experiments.
32235573	5	11	theme	6.5	901:903	arg1	concentration					924:936	initial NH4+-N concentration	909:936	initial NH4+-N concentration of 7 mg/L	909:946	The optimal process parameters of the aforementioned factors were obtained at bed depth of 70 cm, flow rate of 32 mL/min, pH of 6.5 and initial NH4+-N concentration of 7 mg/L.
32235573	5	11	theme	6.5	901:903	arg1	depth					855:859	bed depth	851:859	bed depth of 70 cm	851:868	The optimal process parameters of the aforementioned factors were obtained at bed depth of 70 cm, flow rate of 32 mL/min, pH of 6.5 and initial NH4+-N concentration of 7 mg/L.
32235573	5	11	theme	6.5	901:903	arg1	pH					895:896	pH	895:896	pH of 6.5	895:903	The optimal process parameters of the aforementioned factors were obtained at bed depth of 70 cm, flow rate of 32 mL/min, pH of 6.5 and initial NH4+-N concentration of 7 mg/L.
32235573	5	11	theme	6.5	901:903	arg1	rate					876:879	flow rate	871:879	flow rate of 32 mL/min	871:892	The optimal process parameters of the aforementioned factors were obtained at bed depth of 70 cm, flow rate of 32 mL/min, pH of 6.5 and initial NH4+-N concentration of 7 mg/L.
32235573	3	12	dep	value	540:544	arg1	8.5					560:562	8.5	560:562	8.5	560:562	Effect of bed depth (30, 50 and 70 cm), flow rate (32, 49 and 65 mL/min), initial pH value (4.5, 6.5 and 8.5) and influent NH4+-N concentration (3, 5 and 7 mg/L) was examined by using a filter column packed with CTS-ZMS particles.
32235573	3	12	dep	value	540:544	arg1	6.5					552:554	6.5	552:554	6.5	552:554	Effect of bed depth (30, 50 and 70 cm), flow rate (32, 49 and 65 mL/min), initial pH value (4.5, 6.5 and 8.5) and influent NH4+-N concentration (3, 5 and 7 mg/L) was examined by using a filter column packed with CTS-ZMS particles.
32235573	7	13	dep	showed	1223:1228	arg1	reacted					1321:1327	reacted	1321:1327	showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen	1223:1350	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	0	14	from	Water	99:103	arg1	Removal					86:92	Ammonium Nitrogen Removal	68:92	Ammonium Nitrogen Removal from Water	68:103	Chitosan Modified Zeolite Molecular Sieve Particles as a Filter for Ammonium Nitrogen Removal from Water.
32235573	5	15	theme	mL/min	887:892	arg1	concentration					924:936	initial NH4+-N concentration	909:936	initial NH4+-N concentration of 7 mg/L	909:946	The optimal process parameters of the aforementioned factors were obtained at bed depth of 70 cm, flow rate of 32 mL/min, pH of 6.5 and initial NH4+-N concentration of 7 mg/L.
32235573	5	15	theme	mL/min	887:892	arg1	depth					855:859	bed depth	851:859	bed depth of 70 cm	851:868	The optimal process parameters of the aforementioned factors were obtained at bed depth of 70 cm, flow rate of 32 mL/min, pH of 6.5 and initial NH4+-N concentration of 7 mg/L.
32235573	5	15	theme	mL/min	887:892	arg1	pH					895:896	pH	895:896	pH of 6.5	895:903	The optimal process parameters of the aforementioned factors were obtained at bed depth of 70 cm, flow rate of 32 mL/min, pH of 6.5 and initial NH4+-N concentration of 7 mg/L.
32235573	5	15	theme	mL/min	887:892	arg1	rate					876:879	flow rate	871:879	flow rate of 32 mL/min	871:892	The optimal process parameters of the aforementioned factors were obtained at bed depth of 70 cm, flow rate of 32 mL/min, pH of 6.5 and initial NH4+-N concentration of 7 mg/L.
32235573	3	16	theme	value	540:544	arg1	Effect					455:460	Effect	455:460	Effect of bed depth (30, 50 and 70 cm), flow rate (32, 49 and 65 mL/min), initial pH value (4.5, 6.5 and 8.5) and influent NH4+-N concentration (3, 5 and 7 mg/L)	455:615	Effect of bed depth (30, 50 and 70 cm), flow rate (32, 49 and 65 mL/min), initial pH value (4.5, 6.5 and 8.5) and influent NH4+-N concentration (3, 5 and 7 mg/L) was examined by using a filter column packed with CTS-ZMS particles.
32235573	5	17	theme	process	785:791	arg1	parameters					793:802	The optimal process parameters	773:802	The optimal process parameters of the aforementioned factors	773:832	The optimal process parameters of the aforementioned factors were obtained at bed depth of 70 cm, flow rate of 32 mL/min, pH of 6.5 and initial NH4+-N concentration of 7 mg/L.
32235573	3	18	theme	filter	641:646	arg1	column					648:653	a filter column	639:653	a filter column packed with CTS-ZMS particles	639:683	Effect of bed depth (30, 50 and 70 cm), flow rate (32, 49 and 65 mL/min), initial pH value (4.5, 6.5 and 8.5) and influent NH4+-N concentration (3, 5 and 7 mg/L) was examined by using a filter column packed with CTS-ZMS particles.
32235573	6	19	theme	running	1189:1195	arg1	months					1182:1187	3 months running	1180:1195	3 months running	1180:1195	Scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and Fourier Transform Infrared Spectroscopy (FTIR) were investigated to analyze the structure and morphology of the CTS-ZMS adsorbents before and after 3 months running.
32235573	3	20	theme	initial	529:535	arg1	value					540:544	initial pH value	529:544	initial pH value (4.5, 6.5 and 8.5)	529:563	Effect of bed depth (30, 50 and 70 cm), flow rate (32, 49 and 65 mL/min), initial pH value (4.5, 6.5 and 8.5) and influent NH4+-N concentration (3, 5 and 7 mg/L) was examined by using a filter column packed with CTS-ZMS particles.
32235573	0	21	theme	Ammonium	68:75	arg1	Removal					86:92	Ammonium Nitrogen Removal	68:92	Ammonium Nitrogen Removal from Water	68:103	Chitosan Modified Zeolite Molecular Sieve Particles as a Filter for Ammonium Nitrogen Removal from Water.
32235573	1	22	theme	ammonium-nitrogen	149:165	arg1	amount					139:144	a high amount	132:144	a high amount of ammonium-nitrogen (NH4+-N)	132:174	Drinking water containing a high amount of ammonium-nitrogen (NH4+-N) is not effectively removed by conventional treatment processes and can cause eutrophication.
32235573	1	22	theme	ammonium-nitrogen	149:165	arg1	NH4+-N					168:173	NH4+-N	168:173	NH4+-N	168:173	Drinking water containing a high amount of ammonium-nitrogen (NH4+-N) is not effectively removed by conventional treatment processes and can cause eutrophication.
32235573	1	22	theme	ammonium-nitrogen	149:165	arg1	ammonium-nitrogen					149:165	ammonium-nitrogen	149:165	ammonium-nitrogen (NH4+-N)	149:174	Drinking water containing a high amount of ammonium-nitrogen (NH4+-N) is not effectively removed by conventional treatment processes and can cause eutrophication.
32235573	2	23	from	based	309:313	arg1	crosslink					327:335	chitosan crosslink	318:335	chitosan crosslink with zeolite molecular sieve (CTS-ZMS)	318:374	In this research, a composite adsorbent based on chitosan crosslink with zeolite molecular sieve (CTS-ZMS) was prepared for NH4+-N removal through dynamic adsorption filter experiments.
32235573	3	24	theme	CTS-ZMS	667:673	arg1	particles					675:683	CTS-ZMS particles	667:683	CTS-ZMS particles	667:683	Effect of bed depth (30, 50 and 70 cm), flow rate (32, 49 and 65 mL/min), initial pH value (4.5, 6.5 and 8.5) and influent NH4+-N concentration (3, 5 and 7 mg/L) was examined by using a filter column packed with CTS-ZMS particles.
32235573	5	25	theme	initial	909:915	arg1	concentration					924:936	initial NH4+-N concentration	909:936	initial NH4+-N concentration of 7 mg/L	909:946	The optimal process parameters of the aforementioned factors were obtained at bed depth of 70 cm, flow rate of 32 mL/min, pH of 6.5 and initial NH4+-N concentration of 7 mg/L.
32235573	6	26	theme	adsorbents	1152:1161	arg1	structure					1112:1120	structure	1112:1120	structure	1112:1120	Scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and Fourier Transform Infrared Spectroscopy (FTIR) were investigated to analyze the structure and morphology of the CTS-ZMS adsorbents before and after 3 months running.
32235573	6	26	theme	adsorbents	1152:1161	arg1	morphology					1126:1135	morphology	1126:1135	morphology	1126:1135	Scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and Fourier Transform Infrared Spectroscopy (FTIR) were investigated to analyze the structure and morphology of the CTS-ZMS adsorbents before and after 3 months running.
32235573	2	27	theme	adsorption	424:433	arg1	experiments					442:452	dynamic adsorption filter experiments	416:452	dynamic adsorption filter experiments	416:452	In this research, a composite adsorbent based on chitosan crosslink with zeolite molecular sieve (CTS-ZMS) was prepared for NH4+-N removal through dynamic adsorption filter experiments.
32235573	0	28	theme	Zeolite	18:24	arg1	Particles					42:50	Zeolite Molecular Sieve Particles	18:50	Zeolite Molecular Sieve Particles as a Filter for Ammonium Nitrogen Removal from Water	18:103	Chitosan Modified Zeolite Molecular Sieve Particles as a Filter for Ammonium Nitrogen Removal from Water.
32235573	5	29	theme	aforementioned	811:824	arg1	factors					826:832	the aforementioned factors	807:832	the aforementioned factors	807:832	The optimal process parameters of the aforementioned factors were obtained at bed depth of 70 cm, flow rate of 32 mL/min, pH of 6.5 and initial NH4+-N concentration of 7 mg/L.
32235573	2	30	theme	NH4+-N	393:398	arg1	removal					400:406	NH4+-N removal	393:406	NH4+-N removal through dynamic adsorption filter experiments	393:452	In this research, a composite adsorbent based on chitosan crosslink with zeolite molecular sieve (CTS-ZMS) was prepared for NH4+-N removal through dynamic adsorption filter experiments.
32235573	0	31	theme	Sieve	36:40	arg1	Particles					42:50	Zeolite Molecular Sieve Particles	18:50	Zeolite Molecular Sieve Particles as a Filter for Ammonium Nitrogen Removal from Water	18:103	Chitosan Modified Zeolite Molecular Sieve Particles as a Filter for Ammonium Nitrogen Removal from Water.
32235573	7	32	theme	adsorbent	1301:1309	arg1	particles					1311:1319	CTS-ZMS adsorbent particles	1293:1319	CTS-ZMS adsorbent particles	1293:1319	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	6	33	theme	X-ray	1003:1007	arg1	EDS					1023:1025	EDS	1023:1025	EDS	1023:1025	Scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and Fourier Transform Infrared Spectroscopy (FTIR) were investigated to analyze the structure and morphology of the CTS-ZMS adsorbents before and after 3 months running.
32235573	6	33	theme	X-ray	1003:1007	arg1	spectroscopy					1009:1020	energy dispersive X-ray spectroscopy	985:1020	energy dispersive X-ray spectroscopy (EDS)	985:1026	Scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and Fourier Transform Infrared Spectroscopy (FTIR) were investigated to analyze the structure and morphology of the CTS-ZMS adsorbents before and after 3 months running.
32235573	3	34	theme	depth	469:473	arg1	Effect					455:460	Effect	455:460	Effect of bed depth (30, 50 and 70 cm), flow rate (32, 49 and 65 mL/min), initial pH value (4.5, 6.5 and 8.5) and influent NH4+-N concentration (3, 5 and 7 mg/L)	455:615	Effect of bed depth (30, 50 and 70 cm), flow rate (32, 49 and 65 mL/min), initial pH value (4.5, 6.5 and 8.5) and influent NH4+-N concentration (3, 5 and 7 mg/L) was examined by using a filter column packed with CTS-ZMS particles.
32235573	8	35	theme	water	1428:1432	arg1	processes					1445:1453	drinking water filtration processes	1419:1453	drinking water filtration processes	1419:1453	The results of this study supported the use of CTS-ZMS to improve drinking water filtration processes by increasing ammonium nitrogen reductions.
32235573	1	36	theme	conventional	206:217	arg1	processes					229:237	conventional treatment processes	206:237	conventional treatment processes	206:237	Drinking water containing a high amount of ammonium-nitrogen (NH4+-N) is not effectively removed by conventional treatment processes and can cause eutrophication.
32235573	7	37	from	-COO-	1284:1288	arg1	particles					1311:1319	CTS-ZMS adsorbent particles	1293:1319	CTS-ZMS adsorbent particles	1293:1319	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	1	38	contain	containing	121:130	arg2	ammonium-nitrogen					149:165	ammonium-nitrogen	149:165	ammonium-nitrogen (NH4+-N)	149:174	Drinking water containing a high amount of ammonium-nitrogen (NH4+-N) is not effectively removed by conventional treatment processes and can cause eutrophication.
32235573	1	38	contain	containing	121:130	arg2	amount					139:144	a high amount	132:144	a high amount of ammonium-nitrogen (NH4+-N)	132:174	Drinking water containing a high amount of ammonium-nitrogen (NH4+-N) is not effectively removed by conventional treatment processes and can cause eutrophication.
32235573	1	38	contain	containing	121:130	arg1	water					115:119	Drinking water	106:119	Drinking water containing a high amount of ammonium-nitrogen (NH4+-N)	106:174	Drinking water containing a high amount of ammonium-nitrogen (NH4+-N) is not effectively removed by conventional treatment processes and can cause eutrophication.
32235573	1	38	contain	containing	121:130	arg2	NH4+-N					168:173	NH4+-N	168:173	NH4+-N	168:173	Drinking water containing a high amount of ammonium-nitrogen (NH4+-N) is not effectively removed by conventional treatment processes and can cause eutrophication.
32235573	2	39	theme	molecular	350:358	arg1	CTS-ZMS					367:373	CTS-ZMS	367:373	CTS-ZMS	367:373	In this research, a composite adsorbent based on chitosan crosslink with zeolite molecular sieve (CTS-ZMS) was prepared for NH4+-N removal through dynamic adsorption filter experiments.
32235573	2	39	theme	molecular	350:358	arg1	sieve					360:364	zeolite molecular sieve	342:364	zeolite molecular sieve (CTS-ZMS)	342:374	In this research, a composite adsorbent based on chitosan crosslink with zeolite molecular sieve (CTS-ZMS) was prepared for NH4+-N removal through dynamic adsorption filter experiments.
32235573	8	40	theme	CTS-ZMS	1400:1406	arg1	use					1393:1395	the use	1389:1395	the use of CTS-ZMS to improve drinking water filtration processes by increasing ammonium nitrogen reductions	1389:1496	The results of this study supported the use of CTS-ZMS to improve drinking water filtration processes by increasing ammonium nitrogen reductions.
32235573	3	41	theme	NH4+-N	578:583	arg1	concentration					585:597	influent NH4+-N concentration	569:597	influent NH4+-N concentration (3, 5 and 7 mg/L)	569:615	Effect of bed depth (30, 50 and 70 cm), flow rate (32, 49 and 65 mL/min), initial pH value (4.5, 6.5 and 8.5) and influent NH4+-N concentration (3, 5 and 7 mg/L) was examined by using a filter column packed with CTS-ZMS particles.
32235573	3	41	theme	NH4+-N	578:583	arg1	mg/L					611:614	3, 5 and 7 mg/L	600:614	3, 5 and 7 mg/L	600:614	Effect of bed depth (30, 50 and 70 cm), flow rate (32, 49 and 65 mL/min), initial pH value (4.5, 6.5 and 8.5) and influent NH4+-N concentration (3, 5 and 7 mg/L) was examined by using a filter column packed with CTS-ZMS particles.
32235573	3	42	theme	flow	495:498	arg1	mL/min					520:525	32, 49 and 65 mL/min	506:525	32, 49 and 65 mL/min	506:525	Effect of bed depth (30, 50 and 70 cm), flow rate (32, 49 and 65 mL/min), initial pH value (4.5, 6.5 and 8.5) and influent NH4+-N concentration (3, 5 and 7 mg/L) was examined by using a filter column packed with CTS-ZMS particles.
32235573	3	42	theme	flow	495:498	arg1	rate					500:503	flow rate	495:503	flow rate (32, 49 and 65 mL/min)	495:526	Effect of bed depth (30, 50 and 70 cm), flow rate (32, 49 and 65 mL/min), initial pH value (4.5, 6.5 and 8.5) and influent NH4+-N concentration (3, 5 and 7 mg/L) was examined by using a filter column packed with CTS-ZMS particles.
32235573	8	43	theme	nitrogen	1478:1485	arg1	reductions					1487:1496	ammonium nitrogen reductions	1469:1496	ammonium nitrogen reductions	1469:1496	The results of this study supported the use of CTS-ZMS to improve drinking water filtration processes by increasing ammonium nitrogen reductions.
32235573	7	44	from	particles	1311:1319	arg1	-OH					1270:1272	-OH	1270:1272	-OH	1270:1272	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	7	44	from	particles	1311:1319	arg1	groups					1260:1265	the active functional groups	1238:1265	the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles	1238:1319	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	7	44	from	particles	1311:1319	arg1	Na+					1230:1232	Na+	1230:1232	Na+	1230:1232	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	7	44	from	particles	1311:1319	arg1	-COO-					1284:1288	-COO-	1284:1288	-COO-	1284:1288	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	7	44	from	particles	1311:1319	arg1	-NH2					1275:1278	-NH2	1275:1278	-NH2	1275:1278	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	7	45	theme	functional	1249:1258	arg1	-OH					1270:1272	-OH	1270:1272	-OH	1270:1272	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	7	45	theme	functional	1249:1258	arg1	groups					1260:1265	the active functional groups	1238:1265	the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles	1238:1319	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	7	45	theme	functional	1249:1258	arg1	-COO-					1284:1288	-COO-	1284:1288	-COO-	1284:1288	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	7	45	theme	functional	1249:1258	arg1	-NH2					1275:1278	-NH2	1275:1278	-NH2	1275:1278	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	6	46	theme	energy	985:990	arg1	EDS					1023:1025	EDS	1023:1025	EDS	1023:1025	Scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and Fourier Transform Infrared Spectroscopy (FTIR) were investigated to analyze the structure and morphology of the CTS-ZMS adsorbents before and after 3 months running.
32235573	6	46	theme	energy	985:990	arg1	spectroscopy					1009:1020	energy dispersive X-ray spectroscopy	985:1020	energy dispersive X-ray spectroscopy (EDS)	985:1026	Scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and Fourier Transform Infrared Spectroscopy (FTIR) were investigated to analyze the structure and morphology of the CTS-ZMS adsorbents before and after 3 months running.
32235573	4	47	theme	Thomas	690:695	arg1	model					697:701	The Thomas model	686:701	The Thomas model	686:701	The Thomas model was applied to study the breakthrough curves and adsorption capacity.
32235573	7	48	theme	FTIR	1210:1213	arg1	results					1215:1221	The EDS and FTIR results	1198:1221	results	1215:1221	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	6	49	theme	CTS-ZMS	1144:1150	arg1	adsorbents					1152:1161	the CTS-ZMS adsorbents	1140:1161	the CTS-ZMS adsorbents before and after 3 months running	1140:1195	Scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and Fourier Transform Infrared Spectroscopy (FTIR) were investigated to analyze the structure and morphology of the CTS-ZMS adsorbents before and after 3 months running.
32235573	2	50	theme	composite	289:297	arg1	based					309:313	a composite adsorbent based	287:313	a composite adsorbent based on chitosan crosslink with zeolite molecular sieve (CTS-ZMS)	287:374	In this research, a composite adsorbent based on chitosan crosslink with zeolite molecular sieve (CTS-ZMS) was prepared for NH4+-N removal through dynamic adsorption filter experiments.
32235573	6	51	dep	structure	1112:1120	arg1	the					1108:1110	the	1108:1110	the	1108:1110	Scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and Fourier Transform Infrared Spectroscopy (FTIR) were investigated to analyze the structure and morphology of the CTS-ZMS adsorbents before and after 3 months running.
32235573	6	52	theme	electron	958:965	arg1	SEM					979:981	SEM	979:981	SEM	979:981	Scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and Fourier Transform Infrared Spectroscopy (FTIR) were investigated to analyze the structure and morphology of the CTS-ZMS adsorbents before and after 3 months running.
32235573	6	52	theme	electron	958:965	arg1	microscopy					967:976	Scanning electron microscopy	949:976	Scanning electron microscopy (SEM)	949:982	Scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and Fourier Transform Infrared Spectroscopy (FTIR) were investigated to analyze the structure and morphology of the CTS-ZMS adsorbents before and after 3 months running.
32235573	7	53	from	Na+	1230:1232	arg1	particles					1311:1319	CTS-ZMS adsorbent particles	1293:1319	CTS-ZMS adsorbent particles	1293:1319	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	7	54	from	-OH	1270:1272	arg1	particles					1311:1319	CTS-ZMS adsorbent particles	1293:1319	CTS-ZMS adsorbent particles	1293:1319	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	1	55	theme	Drinking	106:113	arg1	water					115:119	Drinking water	106:119	Drinking water containing a high amount of ammonium-nitrogen (NH4+-N)	106:174	Drinking water containing a high amount of ammonium-nitrogen (NH4+-N) is not effectively removed by conventional treatment processes and can cause eutrophication.
32235573	7	56	from	-NH2	1275:1278	arg1	particles					1311:1319	CTS-ZMS adsorbent particles	1293:1319	CTS-ZMS adsorbent particles	1293:1319	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	3	57	theme	pH	537:538	arg1	value					540:544	initial pH value	529:544	initial pH value (4.5, 6.5 and 8.5)	529:563	Effect of bed depth (30, 50 and 70 cm), flow rate (32, 49 and 65 mL/min), initial pH value (4.5, 6.5 and 8.5) and influent NH4+-N concentration (3, 5 and 7 mg/L) was examined by using a filter column packed with CTS-ZMS particles.
32235573	5	58	theme	optimal	777:783	arg1	parameters					793:802	The optimal process parameters	773:802	The optimal process parameters of the aforementioned factors	773:832	The optimal process parameters of the aforementioned factors were obtained at bed depth of 70 cm, flow rate of 32 mL/min, pH of 6.5 and initial NH4+-N concentration of 7 mg/L.
32235573	4	59	theme	adsorption	752:761	arg1	capacity					763:770	adsorption capacity	752:770	adsorption capacity	752:770	The Thomas model was applied to study the breakthrough curves and adsorption capacity.
32235573	2	60	theme	chitosan	318:325	arg1	crosslink					327:335	chitosan crosslink	318:335	chitosan crosslink with zeolite molecular sieve (CTS-ZMS)	318:374	In this research, a composite adsorbent based on chitosan crosslink with zeolite molecular sieve (CTS-ZMS) was prepared for NH4+-N removal through dynamic adsorption filter experiments.
32235573	1	61	theme	high	134:137	arg1	amount					139:144	a high amount	132:144	a high amount of ammonium-nitrogen (NH4+-N)	132:174	Drinking water containing a high amount of ammonium-nitrogen (NH4+-N) is not effectively removed by conventional treatment processes and can cause eutrophication.
32235573	1	61	theme	high	134:137	arg1	NH4+-N					168:173	NH4+-N	168:173	NH4+-N	168:173	Drinking water containing a high amount of ammonium-nitrogen (NH4+-N) is not effectively removed by conventional treatment processes and can cause eutrophication.
32235573	1	61	theme	high	134:137	arg1	ammonium-nitrogen					149:165	ammonium-nitrogen	149:165	ammonium-nitrogen (NH4+-N)	149:174	Drinking water containing a high amount of ammonium-nitrogen (NH4+-N) is not effectively removed by conventional treatment processes and can cause eutrophication.
32235573	7	62	from	groups	1260:1265	arg1	particles					1311:1319	CTS-ZMS adsorbent particles	1293:1319	CTS-ZMS adsorbent particles	1293:1319	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	0	63	theme	Nitrogen	77:84	arg1	Removal					86:92	Ammonium Nitrogen Removal	68:92	Ammonium Nitrogen Removal from Water	68:103	Chitosan Modified Zeolite Molecular Sieve Particles as a Filter for Ammonium Nitrogen Removal from Water.
32235573	5	64	theme	mg/L	943:946	arg1	concentration					924:936	initial NH4+-N concentration	909:936	initial NH4+-N concentration of 7 mg/L	909:946	The optimal process parameters of the aforementioned factors were obtained at bed depth of 70 cm, flow rate of 32 mL/min, pH of 6.5 and initial NH4+-N concentration of 7 mg/L.
32235573	5	64	theme	mg/L	943:946	arg1	depth					855:859	bed depth	851:859	bed depth of 70 cm	851:868	The optimal process parameters of the aforementioned factors were obtained at bed depth of 70 cm, flow rate of 32 mL/min, pH of 6.5 and initial NH4+-N concentration of 7 mg/L.
32235573	5	64	theme	mg/L	943:946	arg1	pH					895:896	pH	895:896	pH of 6.5	895:903	The optimal process parameters of the aforementioned factors were obtained at bed depth of 70 cm, flow rate of 32 mL/min, pH of 6.5 and initial NH4+-N concentration of 7 mg/L.
32235573	5	64	theme	mg/L	943:946	arg1	rate					876:879	flow rate	871:879	flow rate of 32 mL/min	871:892	The optimal process parameters of the aforementioned factors were obtained at bed depth of 70 cm, flow rate of 32 mL/min, pH of 6.5 and initial NH4+-N concentration of 7 mg/L.
32235573	6	65	dep	Fourier	1032:1038	arg1	Transform					1040:1048	Transform	1040:1048	Transform Infrared Spectroscopy (FTIR)	1040:1077	Scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and Fourier Transform Infrared Spectroscopy (FTIR) were investigated to analyze the structure and morphology of the CTS-ZMS adsorbents before and after 3 months running.
32235573	5	66	theme	factors	826:832	arg1	parameters					793:802	The optimal process parameters	773:802	The optimal process parameters of the aforementioned factors	773:832	The optimal process parameters of the aforementioned factors were obtained at bed depth of 70 cm, flow rate of 32 mL/min, pH of 6.5 and initial NH4+-N concentration of 7 mg/L.
32235573	7	67	theme	EDS	1202:1204	arg1	results					1215:1221	The EDS and FTIR results	1198:1221	results	1215:1221	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	7	68	theme	ammonium	1334:1341	arg1	nitrogen					1343:1350	ammonium nitrogen	1334:1350	ammonium nitrogen	1334:1350	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	5	69	theme	NH4+-N	917:922	arg1	concentration					924:936	initial NH4+-N concentration	909:936	initial NH4+-N concentration of 7 mg/L	909:946	The optimal process parameters of the aforementioned factors were obtained at bed depth of 70 cm, flow rate of 32 mL/min, pH of 6.5 and initial NH4+-N concentration of 7 mg/L.
32235573	2	70	theme	filter	435:440	arg1	experiments					442:452	dynamic adsorption filter experiments	416:452	dynamic adsorption filter experiments	416:452	In this research, a composite adsorbent based on chitosan crosslink with zeolite molecular sieve (CTS-ZMS) was prepared for NH4+-N removal through dynamic adsorption filter experiments.
32235573	0	71	theme	Molecular	26:34	arg1	Particles					42:50	Zeolite Molecular Sieve Particles	18:50	Zeolite Molecular Sieve Particles as a Filter for Ammonium Nitrogen Removal from Water	18:103	Chitosan Modified Zeolite Molecular Sieve Particles as a Filter for Ammonium Nitrogen Removal from Water.
32235573	2	72	theme	dynamic	416:422	arg1	experiments					442:452	dynamic adsorption filter experiments	416:452	dynamic adsorption filter experiments	416:452	In this research, a composite adsorbent based on chitosan crosslink with zeolite molecular sieve (CTS-ZMS) was prepared for NH4+-N removal through dynamic adsorption filter experiments.
32235573	3	73	theme	bed	465:467	arg1	depth					469:473	bed depth	465:473	bed depth (30, 50 and 70 cm)	465:492	Effect of bed depth (30, 50 and 70 cm), flow rate (32, 49 and 65 mL/min), initial pH value (4.5, 6.5 and 8.5) and influent NH4+-N concentration (3, 5 and 7 mg/L) was examined by using a filter column packed with CTS-ZMS particles.
32235573	3	73	theme	bed	465:467	arg1	cm					490:491	30, 50 and 70 cm	476:491	30, 50 and 70 cm	476:491	Effect of bed depth (30, 50 and 70 cm), flow rate (32, 49 and 65 mL/min), initial pH value (4.5, 6.5 and 8.5) and influent NH4+-N concentration (3, 5 and 7 mg/L) was examined by using a filter column packed with CTS-ZMS particles.
32235573	5	74	theme	cm	867:868	arg1	concentration					924:936	initial NH4+-N concentration	909:936	initial NH4+-N concentration of 7 mg/L	909:946	The optimal process parameters of the aforementioned factors were obtained at bed depth of 70 cm, flow rate of 32 mL/min, pH of 6.5 and initial NH4+-N concentration of 7 mg/L.
32235573	5	74	theme	cm	867:868	arg1	depth					855:859	bed depth	851:859	bed depth of 70 cm	851:868	The optimal process parameters of the aforementioned factors were obtained at bed depth of 70 cm, flow rate of 32 mL/min, pH of 6.5 and initial NH4+-N concentration of 7 mg/L.
32235573	5	74	theme	cm	867:868	arg1	pH					895:896	pH	895:896	pH of 6.5	895:903	The optimal process parameters of the aforementioned factors were obtained at bed depth of 70 cm, flow rate of 32 mL/min, pH of 6.5 and initial NH4+-N concentration of 7 mg/L.
32235573	5	74	theme	cm	867:868	arg1	rate					876:879	flow rate	871:879	flow rate of 32 mL/min	871:892	The optimal process parameters of the aforementioned factors were obtained at bed depth of 70 cm, flow rate of 32 mL/min, pH of 6.5 and initial NH4+-N concentration of 7 mg/L.
32235573	2	75	with	crosslink	327:335	arg1	CTS-ZMS					367:373	CTS-ZMS	367:373	CTS-ZMS	367:373	In this research, a composite adsorbent based on chitosan crosslink with zeolite molecular sieve (CTS-ZMS) was prepared for NH4+-N removal through dynamic adsorption filter experiments.
32235573	2	75	with	crosslink	327:335	arg1	sieve					360:364	zeolite molecular sieve	342:364	zeolite molecular sieve (CTS-ZMS)	342:374	In this research, a composite adsorbent based on chitosan crosslink with zeolite molecular sieve (CTS-ZMS) was prepared for NH4+-N removal through dynamic adsorption filter experiments.
32235573	5	76	theme	flow	871:874	arg1	rate					876:879	flow rate	871:879	flow rate of 32 mL/min	871:892	The optimal process parameters of the aforementioned factors were obtained at bed depth of 70 cm, flow rate of 32 mL/min, pH of 6.5 and initial NH4+-N concentration of 7 mg/L.
32235573	6	77	theme	Scanning	949:956	arg1	SEM					979:981	SEM	979:981	SEM	979:981	Scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and Fourier Transform Infrared Spectroscopy (FTIR) were investigated to analyze the structure and morphology of the CTS-ZMS adsorbents before and after 3 months running.
32235573	6	77	theme	Scanning	949:956	arg1	microscopy					967:976	Scanning electron microscopy	949:976	Scanning electron microscopy (SEM)	949:982	Scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS) and Fourier Transform Infrared Spectroscopy (FTIR) were investigated to analyze the structure and morphology of the CTS-ZMS adsorbents before and after 3 months running.
32235573	8	78	theme	study	1373:1377	arg1	results					1357:1363	The results	1353:1363	The results of this study	1353:1377	The results of this study supported the use of CTS-ZMS to improve drinking water filtration processes by increasing ammonium nitrogen reductions.
32235573	8	79	theme	filtration	1434:1443	arg1	processes					1445:1453	drinking water filtration processes	1419:1453	drinking water filtration processes	1419:1453	The results of this study supported the use of CTS-ZMS to improve drinking water filtration processes by increasing ammonium nitrogen reductions.
32235573	7	80	theme	CTS-ZMS	1293:1299	arg1	particles					1311:1319	CTS-ZMS adsorbent particles	1293:1319	CTS-ZMS adsorbent particles	1293:1319	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	5	81	theme	bed	851:853	arg1	depth					855:859	bed depth	851:859	bed depth of 70 cm	851:868	The optimal process parameters of the aforementioned factors were obtained at bed depth of 70 cm, flow rate of 32 mL/min, pH of 6.5 and initial NH4+-N concentration of 7 mg/L.
32235573	1	82	theme	treatment	219:227	arg1	processes					229:237	conventional treatment processes	206:237	conventional treatment processes	206:237	Drinking water containing a high amount of ammonium-nitrogen (NH4+-N) is not effectively removed by conventional treatment processes and can cause eutrophication.
32235573	7	83	theme	-COO-	1284:1288	arg1	-OH					1270:1272	-OH	1270:1272	-OH	1270:1272	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	7	83	theme	-COO-	1284:1288	arg1	groups					1260:1265	the active functional groups	1238:1265	the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles	1238:1319	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	7	83	theme	-COO-	1284:1288	arg1	Na+					1230:1232	Na+	1230:1232	Na+	1230:1232	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	7	83	theme	-COO-	1284:1288	arg1	-COO-					1284:1288	-COO-	1284:1288	-COO-	1284:1288	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	7	83	theme	-COO-	1284:1288	arg1	-NH2					1275:1278	-NH2	1275:1278	-NH2	1275:1278	The EDS and FTIR results showed Na+ and the active functional groups of -OH, -NH2 and -COO- on CTS-ZMS adsorbent particles reacted with ammonium nitrogen.
32235573	3	84	theme	concentration	585:597	arg1	Effect					455:460	Effect	455:460	Effect of bed depth (30, 50 and 70 cm), flow rate (32, 49 and 65 mL/min), initial pH value (4.5, 6.5 and 8.5) and influent NH4+-N concentration (3, 5 and 7 mg/L)	455:615	Effect of bed depth (30, 50 and 70 cm), flow rate (32, 49 and 65 mL/min), initial pH value (4.5, 6.5 and 8.5) and influent NH4+-N concentration (3, 5 and 7 mg/L) was examined by using a filter column packed with CTS-ZMS particles.
32235573	8	85	theme	ammonium	1469:1476	arg1	reductions					1487:1496	ammonium nitrogen reductions	1469:1496	ammonium nitrogen reductions	1469:1496	The results of this study supported the use of CTS-ZMS to improve drinking water filtration processes by increasing ammonium nitrogen reductions.
34117926	7	0	theme	thermal	1240:1246	arg1	stability					1248:1256	similar thermal stability	1232:1256	similar thermal stability	1232:1256	In addition, exhibiting similar thermal stability as pure cellulose films with antibacterial properties tested against S. aureus and E. coli with good metabolic cellular viability while also inhibiting NF-κB activity, a characteristic of tannic acid.
34117926	7	0	theme	thermal	1240:1246	arg1	films					1276:1280	pure cellulose films	1261:1280	pure cellulose films with antibacterial properties tested against S. aureus and E. coli with good metabolic cellular viability while also inhibiting NF-κB activity, a characteristic of tannic acid	1261:1456	In addition, exhibiting similar thermal stability as pure cellulose films with antibacterial properties tested against S. aureus and E. coli with good metabolic cellular viability while also inhibiting NF-κB activity, a characteristic of tannic acid.
34117926	5	1	with	rigidity	934:941	arg1	structure					959:967	a lamellar structure	948:967	a lamellar structure when incorporated with tannic acid due to its strong hydrogen bonding	948:1037	This film results in increased rigidity with a lamellar structure when incorporated with tannic acid due to its strong hydrogen bonding.
34117926	3	2	theme	regenerative	525:536	arg1	properties					538:547	its antibacterial and regenerative properties	503:547	its antibacterial and regenerative properties	503:547	However, investigation of the crosslink structure and its antibacterial and regenerative properties are still unknown when using nanocellulose by mechanical defibrillation; additionally, due to the potential crosslink structure with chitosan, its structure can be complex.
34117926	0	3	theme	healing	79:85	arg1	applications					87:98	wound healing applications	73:98	wound healing applications	73:98	Microfibrillated cellulose films containing chitosan and tannic acid for wound healing applications.
34117926	7	4	with	films	1276:1280	arg1	properties					1301:1310	antibacterial properties	1287:1310	antibacterial properties tested against S. aureus and E. coli with good metabolic cellular viability while also inhibiting NF-κB activity, a characteristic of tannic acid	1287:1456	In addition, exhibiting similar thermal stability as pure cellulose films with antibacterial properties tested against S. aureus and E. coli with good metabolic cellular viability while also inhibiting NF-κB activity, a characteristic of tannic acid.
34117926	3	5	theme	properties	538:547	arg1	investigation					458:470	investigation	458:470	investigation of the crosslink structure and its antibacterial and regenerative properties	458:547	However, investigation of the crosslink structure and its antibacterial and regenerative properties are still unknown when using nanocellulose by mechanical defibrillation; additionally, due to the potential crosslink structure with chitosan, its structure can be complex.
34117926	4	6	from	effect	803:808	arg1	properties					843:852	the physical and regenerative properties	813:852	the physical and regenerative properties when incorporated with chitosan and tannic acid	813:900	Therefore, this work uses bleach kraft nanocellulose in order to investigate the effect on the physical and regenerative properties when incorporated with chitosan and tannic acid.
34117926	5	7	theme	increased	924:932	arg1	rigidity					934:941	increased rigidity	924:941	increased rigidity with a lamellar structure when incorporated with tannic acid due to its strong hydrogen bonding	924:1037	This film results in increased rigidity with a lamellar structure when incorporated with tannic acid due to its strong hydrogen bonding.
34117926	6	8	theme	release	1166:1172	arg1	model					1174:1178	good release model	1161:1178	good release model	1161:1178	The release of tannic acid varied depending on the structure it was synthesised with, whereas with chitosan it presented good release model compared to pure cellulose.
34117926	7	9	theme	tannic	1446:1451	arg1	acid					1453:1456	tannic acid	1446:1456	tannic acid	1446:1456	In addition, exhibiting similar thermal stability as pure cellulose films with antibacterial properties tested against S. aureus and E. coli with good metabolic cellular viability while also inhibiting NF-κB activity, a characteristic of tannic acid.
34117926	7	10	theme	cellulose	1266:1274	arg1	stability					1248:1256	similar thermal stability	1232:1256	similar thermal stability	1232:1256	In addition, exhibiting similar thermal stability as pure cellulose films with antibacterial properties tested against S. aureus and E. coli with good metabolic cellular viability while also inhibiting NF-κB activity, a characteristic of tannic acid.
34117926	7	10	theme	cellulose	1266:1274	arg1	films					1276:1280	pure cellulose films	1261:1280	pure cellulose films with antibacterial properties tested against S. aureus and E. coli with good metabolic cellular viability while also inhibiting NF-κB activity, a characteristic of tannic acid	1261:1456	In addition, exhibiting similar thermal stability as pure cellulose films with antibacterial properties tested against S. aureus and E. coli with good metabolic cellular viability while also inhibiting NF-κB activity, a characteristic of tannic acid.
34117926	1	11	theme	tannic	122:127	arg1	acid					129:132	tannic acid	122:132	tannic acid	122:132	The effectiveness of tannic acid as antimicrobial and wound healing for burns have been shown for a century; however, uncontrolled target dosage may result in undesirable side-effects.
34117926	2	12	theme	beneficial	411:420	arg1	properties					422:431	the beneficial properties	407:431	the beneficial properties of this tannin	407:446	Remarkably, tannic acid polyphenols compounds crosslinked with polymeric materials produce a strong composite containing the beneficial properties of this tannin.
34117926	5	13	theme	strong	1015:1020	arg1	bonding					1031:1037	its strong hydrogen bonding	1011:1037	its strong hydrogen bonding	1011:1037	This film results in increased rigidity with a lamellar structure when incorporated with tannic acid due to its strong hydrogen bonding.
34117926	3	14	theme	antibacterial	507:519	arg1	properties					538:547	its antibacterial and regenerative properties	503:547	its antibacterial and regenerative properties	503:547	However, investigation of the crosslink structure and its antibacterial and regenerative properties are still unknown when using nanocellulose by mechanical defibrillation; additionally, due to the potential crosslink structure with chitosan, its structure can be complex.
34117926	7	15	theme	cellular	1369:1376	arg1	viability					1378:1386	good metabolic cellular viability	1354:1386	good metabolic cellular viability while also inhibiting NF-κB activity	1354:1423	In addition, exhibiting similar thermal stability as pure cellulose films with antibacterial properties tested against S. aureus and E. coli with good metabolic cellular viability while also inhibiting NF-κB activity, a characteristic of tannic acid.
34117926	7	15	theme	cellular	1369:1376	arg1	characteristic					1428:1441	a characteristic	1426:1441	a characteristic of tannic acid	1426:1456	In addition, exhibiting similar thermal stability as pure cellulose films with antibacterial properties tested against S. aureus and E. coli with good metabolic cellular viability while also inhibiting NF-κB activity, a characteristic of tannic acid.
34117926	0	16	theme	cellulose	17:25	arg1	films					27:31	Microfibrillated cellulose films	0:31	Microfibrillated cellulose films	0:31	Microfibrillated cellulose films containing chitosan and tannic acid for wound healing applications.
34117926	3	17	with	structure	667:675	arg1	chitosan					682:689	chitosan	682:689	chitosan	682:689	However, investigation of the crosslink structure and its antibacterial and regenerative properties are still unknown when using nanocellulose by mechanical defibrillation; additionally, due to the potential crosslink structure with chitosan, its structure can be complex.
34117926	2	18	theme	polyphenols	310:320	arg1	compounds					322:330	tannic acid polyphenols compounds	298:330	tannic acid polyphenols compounds crosslinked with polymeric materials	298:367	Remarkably, tannic acid polyphenols compounds crosslinked with polymeric materials produce a strong composite containing the beneficial properties of this tannin.
34117926	0	19	theme	Microfibrillated	0:15	arg1	films					27:31	Microfibrillated cellulose films	0:31	Microfibrillated cellulose films	0:31	Microfibrillated cellulose films containing chitosan and tannic acid for wound healing applications.
34117926	1	20	theme	undesirable	260:270	arg1	side-effects					272:283	undesirable side-effects	260:283	undesirable side-effects	260:283	The effectiveness of tannic acid as antimicrobial and wound healing for burns have been shown for a century; however, uncontrolled target dosage may result in undesirable side-effects.
34117926	4	21	theme	tannic	890:895	arg1	acid					897:900	tannic acid	890:900	tannic acid	890:900	Therefore, this work uses bleach kraft nanocellulose in order to investigate the effect on the physical and regenerative properties when incorporated with chitosan and tannic acid.
34117926	2	22	theme	acid	305:308	arg1	compounds					322:330	tannic acid polyphenols compounds	298:330	tannic acid polyphenols compounds crosslinked with polymeric materials	298:367	Remarkably, tannic acid polyphenols compounds crosslinked with polymeric materials produce a strong composite containing the beneficial properties of this tannin.
34117926	7	23	theme	good	1354:1357	arg1	viability					1378:1386	good metabolic cellular viability	1354:1386	good metabolic cellular viability while also inhibiting NF-κB activity	1354:1423	In addition, exhibiting similar thermal stability as pure cellulose films with antibacterial properties tested against S. aureus and E. coli with good metabolic cellular viability while also inhibiting NF-κB activity, a characteristic of tannic acid.
34117926	7	23	theme	good	1354:1357	arg1	characteristic					1428:1441	a characteristic	1426:1441	a characteristic of tannic acid	1426:1456	In addition, exhibiting similar thermal stability as pure cellulose films with antibacterial properties tested against S. aureus and E. coli with good metabolic cellular viability while also inhibiting NF-κB activity, a characteristic of tannic acid.
34117926	7	24	theme	acid	1453:1456	arg1	characteristic					1428:1441	a characteristic	1426:1441	a characteristic of tannic acid	1426:1456	In addition, exhibiting similar thermal stability as pure cellulose films with antibacterial properties tested against S. aureus and E. coli with good metabolic cellular viability while also inhibiting NF-κB activity, a characteristic of tannic acid.
34117926	7	24	theme	acid	1453:1456	arg1	viability					1378:1386	good metabolic cellular viability	1354:1386	good metabolic cellular viability while also inhibiting NF-κB activity	1354:1423	In addition, exhibiting similar thermal stability as pure cellulose films with antibacterial properties tested against S. aureus and E. coli with good metabolic cellular viability while also inhibiting NF-κB activity, a characteristic of tannic acid.
34117926	2	25	theme	tannic	298:303	arg1	compounds					322:330	tannic acid polyphenols compounds	298:330	tannic acid polyphenols compounds crosslinked with polymeric materials	298:367	Remarkably, tannic acid polyphenols compounds crosslinked with polymeric materials produce a strong composite containing the beneficial properties of this tannin.
34117926	3	26	theme	crosslink	479:487	arg1	structure					489:497	the crosslink structure	475:497	the crosslink structure	475:497	However, investigation of the crosslink structure and its antibacterial and regenerative properties are still unknown when using nanocellulose by mechanical defibrillation; additionally, due to the potential crosslink structure with chitosan, its structure can be complex.
34117926	6	27	dep	structure	1091:1099	arg1	synthesised					1108:1118	synthesised	1108:1118	synthesised with	1108:1123	The release of tannic acid varied depending on the structure it was synthesised with, whereas with chitosan it presented good release model compared to pure cellulose.
34117926	3	28	theme	mechanical	595:604	arg1	defibrillation					606:619	mechanical defibrillation	595:619	mechanical defibrillation	595:619	However, investigation of the crosslink structure and its antibacterial and regenerative properties are still unknown when using nanocellulose by mechanical defibrillation; additionally, due to the potential crosslink structure with chitosan, its structure can be complex.
34117926	3	29	theme	structure	489:497	arg1	investigation					458:470	investigation	458:470	investigation of the crosslink structure and its antibacterial and regenerative properties	458:547	However, investigation of the crosslink structure and its antibacterial and regenerative properties are still unknown when using nanocellulose by mechanical defibrillation; additionally, due to the potential crosslink structure with chitosan, its structure can be complex.
34117926	5	30	theme	lamellar	950:957	arg1	structure					959:967	a lamellar structure	948:967	a lamellar structure when incorporated with tannic acid due to its strong hydrogen bonding	948:1037	This film results in increased rigidity with a lamellar structure when incorporated with tannic acid due to its strong hydrogen bonding.
34117926	2	31	contain	containing	396:405	arg1	composite					386:394	a strong composite	377:394	a strong composite containing the beneficial properties of this tannin	377:446	Remarkably, tannic acid polyphenols compounds crosslinked with polymeric materials produce a strong composite containing the beneficial properties of this tannin.
34117926	2	31	contain	containing	396:405	arg2	properties					422:431	the beneficial properties	407:431	the beneficial properties of this tannin	407:446	Remarkably, tannic acid polyphenols compounds crosslinked with polymeric materials produce a strong composite containing the beneficial properties of this tannin.
34117926	7	32	theme	metabolic	1359:1367	arg1	viability					1378:1386	good metabolic cellular viability	1354:1386	good metabolic cellular viability while also inhibiting NF-κB activity	1354:1423	In addition, exhibiting similar thermal stability as pure cellulose films with antibacterial properties tested against S. aureus and E. coli with good metabolic cellular viability while also inhibiting NF-κB activity, a characteristic of tannic acid.
34117926	7	32	theme	metabolic	1359:1367	arg1	characteristic					1428:1441	a characteristic	1426:1441	a characteristic of tannic acid	1426:1456	In addition, exhibiting similar thermal stability as pure cellulose films with antibacterial properties tested against S. aureus and E. coli with good metabolic cellular viability while also inhibiting NF-κB activity, a characteristic of tannic acid.
34117926	7	33	dep	viability	1378:1386	arg1	inhibiting					1399:1408	inhibiting	1399:1408	inhibiting NF-κB activity	1399:1423	In addition, exhibiting similar thermal stability as pure cellulose films with antibacterial properties tested against S. aureus and E. coli with good metabolic cellular viability while also inhibiting NF-κB activity, a characteristic of tannic acid.
34117926	7	34	theme	pure	1261:1264	arg1	stability					1248:1256	similar thermal stability	1232:1256	similar thermal stability	1232:1256	In addition, exhibiting similar thermal stability as pure cellulose films with antibacterial properties tested against S. aureus and E. coli with good metabolic cellular viability while also inhibiting NF-κB activity, a characteristic of tannic acid.
34117926	7	34	theme	pure	1261:1264	arg1	films					1276:1280	pure cellulose films	1261:1280	pure cellulose films with antibacterial properties tested against S. aureus and E. coli with good metabolic cellular viability while also inhibiting NF-κB activity, a characteristic of tannic acid	1261:1456	In addition, exhibiting similar thermal stability as pure cellulose films with antibacterial properties tested against S. aureus and E. coli with good metabolic cellular viability while also inhibiting NF-κB activity, a characteristic of tannic acid.
34117926	7	35	theme	NF-κB	1410:1414	arg1	activity					1416:1423	NF-κB activity	1410:1423	NF-κB activity	1410:1423	In addition, exhibiting similar thermal stability as pure cellulose films with antibacterial properties tested against S. aureus and E. coli with good metabolic cellular viability while also inhibiting NF-κB activity, a characteristic of tannic acid.
34117926	2	36	theme	polymeric	349:357	arg1	materials					359:367	polymeric materials	349:367	polymeric materials	349:367	Remarkably, tannic acid polyphenols compounds crosslinked with polymeric materials produce a strong composite containing the beneficial properties of this tannin.
34117926	0	37	theme	tannic	57:62	arg1	acid					64:67	tannic acid	57:67	tannic acid	57:67	Microfibrillated cellulose films containing chitosan and tannic acid for wound healing applications.
34117926	7	38	theme	similar	1232:1238	arg1	stability					1248:1256	similar thermal stability	1232:1256	similar thermal stability	1232:1256	In addition, exhibiting similar thermal stability as pure cellulose films with antibacterial properties tested against S. aureus and E. coli with good metabolic cellular viability while also inhibiting NF-κB activity, a characteristic of tannic acid.
34117926	7	38	theme	similar	1232:1238	arg1	films					1276:1280	pure cellulose films	1261:1280	pure cellulose films with antibacterial properties tested against S. aureus and E. coli with good metabolic cellular viability while also inhibiting NF-κB activity, a characteristic of tannic acid	1261:1456	In addition, exhibiting similar thermal stability as pure cellulose films with antibacterial properties tested against S. aureus and E. coli with good metabolic cellular viability while also inhibiting NF-κB activity, a characteristic of tannic acid.
34117926	1	39	theme	acid	129:132	arg1	effectiveness					105:117	The effectiveness	101:117	The effectiveness of tannic acid as antimicrobial and wound healing for burns	101:177	The effectiveness of tannic acid as antimicrobial and wound healing for burns have been shown for a century; however, uncontrolled target dosage may result in undesirable side-effects.
34117926	7	40	theme	antibacterial	1287:1299	arg1	properties					1301:1310	antibacterial properties	1287:1310	antibacterial properties tested against S. aureus and E. coli with good metabolic cellular viability while also inhibiting NF-κB activity, a characteristic of tannic acid	1287:1456	In addition, exhibiting similar thermal stability as pure cellulose films with antibacterial properties tested against S. aureus and E. coli with good metabolic cellular viability while also inhibiting NF-κB activity, a characteristic of tannic acid.
34117926	4	41	theme	physical	817:824	arg1	properties					843:852	the physical and regenerative properties	813:852	the physical and regenerative properties when incorporated with chitosan and tannic acid	813:900	Therefore, this work uses bleach kraft nanocellulose in order to investigate the effect on the physical and regenerative properties when incorporated with chitosan and tannic acid.
34117926	3	42	theme	crosslink	657:665	arg1	structure					667:675	the potential crosslink structure	643:675	the potential crosslink structure with chitosan	643:689	However, investigation of the crosslink structure and its antibacterial and regenerative properties are still unknown when using nanocellulose by mechanical defibrillation; additionally, due to the potential crosslink structure with chitosan, its structure can be complex.
34117926	4	43	theme	kraft	755:759	arg1	nanocellulose					761:773	bleach kraft nanocellulose	748:773	bleach kraft nanocellulose in order to investigate the effect on the physical and regenerative properties when incorporated with chitosan and tannic acid	748:900	Therefore, this work uses bleach kraft nanocellulose in order to investigate the effect on the physical and regenerative properties when incorporated with chitosan and tannic acid.
34117926	5	44	theme	tannic	992:997	arg1	acid					999:1002	tannic acid	992:1002	tannic acid due to its strong hydrogen bonding	992:1037	This film results in increased rigidity with a lamellar structure when incorporated with tannic acid due to its strong hydrogen bonding.
34117926	6	45	theme	acid	1062:1065	arg1	release					1044:1050	The release	1040:1050	The release of tannic acid	1040:1065	The release of tannic acid varied depending on the structure it was synthesised with, whereas with chitosan it presented good release model compared to pure cellulose.
34117926	6	46	theme	pure	1192:1195	arg1	cellulose					1197:1205	pure cellulose	1192:1205	pure cellulose	1192:1205	The release of tannic acid varied depending on the structure it was synthesised with, whereas with chitosan it presented good release model compared to pure cellulose.
34117926	4	47	theme	bleach	748:753	arg1	nanocellulose					761:773	bleach kraft nanocellulose	748:773	bleach kraft nanocellulose in order to investigate the effect on the physical and regenerative properties when incorporated with chitosan and tannic acid	748:900	Therefore, this work uses bleach kraft nanocellulose in order to investigate the effect on the physical and regenerative properties when incorporated with chitosan and tannic acid.
34117926	6	48	theme	good	1161:1164	arg1	model					1174:1178	good release model	1161:1178	good release model	1161:1178	The release of tannic acid varied depending on the structure it was synthesised with, whereas with chitosan it presented good release model compared to pure cellulose.
34117926	6	49	theme	tannic	1055:1060	arg1	acid					1062:1065	tannic acid	1055:1065	tannic acid	1055:1065	The release of tannic acid varied depending on the structure it was synthesised with, whereas with chitosan it presented good release model compared to pure cellulose.
34117926	0	50	theme	wound	73:77	arg1	healing					79:85	wound healing	73:85	wound healing applications	73:98	Microfibrillated cellulose films containing chitosan and tannic acid for wound healing applications.
34117926	4	51	theme	regenerative	830:841	arg1	properties					843:852	the physical and regenerative properties	813:852	the physical and regenerative properties when incorporated with chitosan and tannic acid	813:900	Therefore, this work uses bleach kraft nanocellulose in order to investigate the effect on the physical and regenerative properties when incorporated with chitosan and tannic acid.
34117926	2	52	theme	tannin	441:446	arg1	properties					422:431	the beneficial properties	407:431	the beneficial properties of this tannin	407:446	Remarkably, tannic acid polyphenols compounds crosslinked with polymeric materials produce a strong composite containing the beneficial properties of this tannin.
34117926	2	53	theme	strong	379:384	arg1	composite					386:394	a strong composite	377:394	a strong composite containing the beneficial properties of this tannin	377:446	Remarkably, tannic acid polyphenols compounds crosslinked with polymeric materials produce a strong composite containing the beneficial properties of this tannin.
34117926	1	54	theme	uncontrolled	219:230	arg1	dosage					239:244	uncontrolled target dosage	219:244	uncontrolled target dosage	219:244	The effectiveness of tannic acid as antimicrobial and wound healing for burns have been shown for a century; however, uncontrolled target dosage may result in undesirable side-effects.
34117926	5	55	theme	hydrogen	1022:1029	arg1	bonding					1031:1037	its strong hydrogen bonding	1011:1037	its strong hydrogen bonding	1011:1037	This film results in increased rigidity with a lamellar structure when incorporated with tannic acid due to its strong hydrogen bonding.
34117926	3	56	theme	potential	647:655	arg1	structure					667:675	the potential crosslink structure	643:675	the potential crosslink structure with chitosan	643:689	However, investigation of the crosslink structure and its antibacterial and regenerative properties are still unknown when using nanocellulose by mechanical defibrillation; additionally, due to the potential crosslink structure with chitosan, its structure can be complex.
34117926	1	57	theme	wound	155:159	arg1	healing					161:167	wound healing	155:167	wound healing	155:167	The effectiveness of tannic acid as antimicrobial and wound healing for burns have been shown for a century; however, uncontrolled target dosage may result in undesirable side-effects.
34117926	1	58	theme	target	232:237	arg1	dosage					239:244	uncontrolled target dosage	219:244	uncontrolled target dosage	219:244	The effectiveness of tannic acid as antimicrobial and wound healing for burns have been shown for a century; however, uncontrolled target dosage may result in undesirable side-effects.
33716132	0	0	theme	composite	106:114	arg1	beads					116:120	activated carbon composite beads	89:120	activated carbon composite beads	89:120	Methylene blue contaminated water sanitization with alginate/compact discs waste-derived activated carbon composite beads: Adsorption studies.
33716132	0	1	with	sanitization	34:45	arg1	discs					69:73	alginate/compact discs	52:73	alginate/compact discs	52:73	Methylene blue contaminated water sanitization with alginate/compact discs waste-derived activated carbon composite beads: Adsorption studies.
33716132	1	2	theme	adsorbents	180:189	arg1	behavior					158:165	The adsorption behavior	143:165	The adsorption behavior of different adsorbents	143:189	The adsorption behavior of different adsorbents has been studied by various research works.
33716132	0	3	theme	carbon	99:104	arg1	beads					116:120	activated carbon composite beads	89:120	activated carbon composite beads	89:120	Methylene blue contaminated water sanitization with alginate/compact discs waste-derived activated carbon composite beads: Adsorption studies.
33716132	7	4	theme	error	870:874	arg1	values					885:890	different error function values	860:890	different error function values	860:890	Isotherms and kinetic models in linear and non-linear forms were studied and the results were examined by comparing R2 along with different error function values to find the best fitting.
33716132	4	5	dep	low-cost	475:482	arg1	adsorbent					494:502	adsorbent	494:502	adsorbent	494:502	A low-cost and green adsorbent was fabricated to can easily be detached from water.
33716132	3	6	theme	black	393:397	arg1	carbon					386:391	carbon black	386:397	activated carbon black (ACB)	376:403	Here, the effect of activated carbon black (ACB) on the methylene blue adsorption behavior of alginate was examined.
33716132	3	6	theme	black	393:397	arg1	ACB					400:402	ACB	400:402	ACB	400:402	Here, the effect of activated carbon black (ACB) on the methylene blue adsorption behavior of alginate was examined.
33716132	0	7	dep	waste-derived	75:87	arg1	studies					134:140	Adsorption studies	123:140	Adsorption studies	123:140	Methylene blue contaminated water sanitization with alginate/compact discs waste-derived activated carbon composite beads: Adsorption studies.
33716132	9	8	theme	pore	1141:1144	arg1	diffusion					1146:1154	pore diffusion	1141:1154	pore diffusion	1141:1154	Intraparticle diffusion model and phenomenological coefficients represented control of adsorption by film diffusion and its limiting by pore diffusion.
33716132	6	9	theme	alginate	706:713	arg1	yield					697:701	removal yield	689:701	removal yield of alginate	689:713	Loading of 15 wt% ACB related to pure alginate increased removal yield of alginate significantly.
33716132	3	10	theme	activated	376:384	arg1	carbon					386:391	carbon black	386:397	activated carbon black (ACB)	376:403	Here, the effect of activated carbon black (ACB) on the methylene blue adsorption behavior of alginate was examined.
33716132	3	10	theme	activated	376:384	arg1	ACB					400:402	ACB	400:402	ACB	400:402	Here, the effect of activated carbon black (ACB) on the methylene blue adsorption behavior of alginate was examined.
33716132	9	11	theme	film	1106:1109	arg1	diffusion					1111:1119	film diffusion	1106:1119	film diffusion	1106:1119	Intraparticle diffusion model and phenomenological coefficients represented control of adsorption by film diffusion and its limiting by pore diffusion.
33716132	5	12	theme	ACB	627:629	arg1	preparation					612:622	the preparation	608:622	the preparation of ACB	608:629	For this aim waste compact discs were recycled for the preparation of ACB.
33716132	8	13	theme	linear	988:993	arg1	Langmuir					995:1002	linear Langmuir	988:1002	linear Langmuir	988:1002	The results were well matched with non-linear pseudo-second-order and linear Langmuir.
33716132	2	14	theme	kinetic	300:306	arg1	models					308:313	kinetic models	300:313	kinetic models alongside phenomenological coefficients	300:353	But few studies have compared linear and non-linear isotherm and kinetic models alongside phenomenological coefficients.
33716132	7	15	theme	different	860:868	arg1	values					885:890	different error function values	860:890	different error function values	860:890	Isotherms and kinetic models in linear and non-linear forms were studied and the results were examined by comparing R2 along with different error function values to find the best fitting.
33716132	0	16	theme	Adsorption	123:132	arg1	studies					134:140	Adsorption studies	123:140	Adsorption studies	123:140	Methylene blue contaminated water sanitization with alginate/compact discs waste-derived activated carbon composite beads: Adsorption studies.
33716132	5	17	theme	compact	576:582	arg1	discs					584:588	waste compact discs	570:588	waste compact discs	570:588	For this aim waste compact discs were recycled for the preparation of ACB.
33716132	0	18	theme	blue	10:13	arg1	sanitization					34:45	Methylene blue contaminated water sanitization	0:45	Methylene blue contaminated water sanitization with alginate/compact discs	0:73	Methylene blue contaminated water sanitization with alginate/compact discs waste-derived activated carbon composite beads: Adsorption studies.
33716132	3	19	theme	blue	422:425	arg1	behavior					438:445	the methylene blue adsorption behavior	408:445	the methylene blue adsorption behavior of alginate	408:457	Here, the effect of activated carbon black (ACB) on the methylene blue adsorption behavior of alginate was examined.
33716132	6	20	theme	removal	689:695	arg1	yield					697:701	removal yield	689:701	removal yield of alginate	689:713	Loading of 15 wt% ACB related to pure alginate increased removal yield of alginate significantly.
33716132	2	21	dep	non-linear	276:285	arg1	isotherm					287:294	isotherm	287:294	isotherm	287:294	But few studies have compared linear and non-linear isotherm and kinetic models alongside phenomenological coefficients.
33716132	0	22	theme	water	28:32	arg1	sanitization					34:45	Methylene blue contaminated water sanitization	0:45	Methylene blue contaminated water sanitization with alginate/compact discs	0:73	Methylene blue contaminated water sanitization with alginate/compact discs waste-derived activated carbon composite beads: Adsorption studies.
33716132	1	23	theme	research	219:226	arg1	works					228:232	various research works	211:232	various research works	211:232	The adsorption behavior of different adsorbents has been studied by various research works.
33716132	0	24	theme	contaminated	15:26	arg1	sanitization					34:45	Methylene blue contaminated water sanitization	0:45	Methylene blue contaminated water sanitization with alginate/compact discs	0:73	Methylene blue contaminated water sanitization with alginate/compact discs waste-derived activated carbon composite beads: Adsorption studies.
33716132	2	25	theme	phenomenological	325:340	arg1	coefficients					342:353	phenomenological coefficients	325:353	phenomenological coefficients	325:353	But few studies have compared linear and non-linear isotherm and kinetic models alongside phenomenological coefficients.
33716132	6	26	theme	pure	665:668	arg1	alginate					670:677	pure alginate	665:677	pure alginate	665:677	Loading of 15 wt% ACB related to pure alginate increased removal yield of alginate significantly.
33716132	3	27	theme	adsorption	427:436	arg1	behavior					438:445	the methylene blue adsorption behavior	408:445	the methylene blue adsorption behavior of alginate	408:457	Here, the effect of activated carbon black (ACB) on the methylene blue adsorption behavior of alginate was examined.
33716132	9	28	theme	phenomenological	1039:1054	arg1	coefficients					1056:1067	Intraparticle diffusion model and phenomenological coefficients	1005:1067	coefficients	1056:1067	Intraparticle diffusion model and phenomenological coefficients represented control of adsorption by film diffusion and its limiting by pore diffusion.
33716132	7	29	from	Isotherms	730:738	arg1	non-linear					773:782	non-linear	773:782	non-linear	773:782	Isotherms and kinetic models in linear and non-linear forms were studied and the results were examined by comparing R2 along with different error function values to find the best fitting.
33716132	7	29	from	Isotherms	730:738	arg1	linear					762:767	linear	762:767	linear	762:767	Isotherms and kinetic models in linear and non-linear forms were studied and the results were examined by comparing R2 along with different error function values to find the best fitting.
33716132	9	30	theme	adsorption	1092:1101	arg1	control					1081:1087	control	1081:1087	control of adsorption by film diffusion	1081:1119	Intraparticle diffusion model and phenomenological coefficients represented control of adsorption by film diffusion and its limiting by pore diffusion.
33716132	3	31	theme	alginate	450:457	arg1	behavior					438:445	the methylene blue adsorption behavior	408:445	the methylene blue adsorption behavior of alginate	408:457	Here, the effect of activated carbon black (ACB) on the methylene blue adsorption behavior of alginate was examined.
33716132	7	32	theme	best	904:907	arg1	fitting					909:915	the best fitting	900:915	the best fitting	900:915	Isotherms and kinetic models in linear and non-linear forms were studied and the results were examined by comparing R2 along with different error function values to find the best fitting.
33716132	9	33	theme	Intraparticle	1005:1017	arg1	model					1029:1033	Intraparticle diffusion model and phenomenological coefficients	1005:1067	model	1029:1033	Intraparticle diffusion model and phenomenological coefficients represented control of adsorption by film diffusion and its limiting by pore diffusion.
33716132	3	34	theme	carbon	386:391	arg1	effect					366:371	the effect	362:371	the effect of activated carbon black (ACB) on the methylene blue adsorption behavior of alginate	362:457	Here, the effect of activated carbon black (ACB) on the methylene blue adsorption behavior of alginate was examined.
33716132	9	35	theme	diffusion	1019:1027	arg1	model					1029:1033	Intraparticle diffusion model and phenomenological coefficients	1005:1067	model	1029:1033	Intraparticle diffusion model and phenomenological coefficients represented control of adsorption by film diffusion and its limiting by pore diffusion.
33716132	7	36	dep	linear	762:767	arg1	forms					784:788	forms	784:788	forms	784:788	Isotherms and kinetic models in linear and non-linear forms were studied and the results were examined by comparing R2 along with different error function values to find the best fitting.
33716132	1	37	theme	various	211:217	arg1	works					228:232	various research works	211:232	various research works	211:232	The adsorption behavior of different adsorbents has been studied by various research works.
33716132	0	38	theme	alginate/compact	52:67	arg1	discs					69:73	alginate/compact discs	52:73	alginate/compact discs	52:73	Methylene blue contaminated water sanitization with alginate/compact discs waste-derived activated carbon composite beads: Adsorption studies.
33716132	7	39	from	models	752:757	arg1	non-linear					773:782	non-linear	773:782	non-linear	773:782	Isotherms and kinetic models in linear and non-linear forms were studied and the results were examined by comparing R2 along with different error function values to find the best fitting.
33716132	7	39	from	models	752:757	arg1	linear					762:767	linear	762:767	linear	762:767	Isotherms and kinetic models in linear and non-linear forms were studied and the results were examined by comparing R2 along with different error function values to find the best fitting.
33716132	2	40	theme	few	239:241	arg1	studies					243:249	few studies	239:249	few studies	239:249	But few studies have compared linear and non-linear isotherm and kinetic models alongside phenomenological coefficients.
33716132	6	41	theme	related	654:660	arg1	ACB					650:652	15 wt% ACB	643:652	15 wt% ACB related to pure alginate	643:677	Loading of 15 wt% ACB related to pure alginate increased removal yield of alginate significantly.
33716132	5	42	theme	waste	570:574	arg1	discs					584:588	waste compact discs	570:588	waste compact discs	570:588	For this aim waste compact discs were recycled for the preparation of ACB.
33716132	1	43	theme	adsorption	147:156	arg1	behavior					158:165	The adsorption behavior	143:165	The adsorption behavior of different adsorbents	143:189	The adsorption behavior of different adsorbents has been studied by various research works.
33716132	6	44	theme	ACB	650:652	arg1	Loading					632:638	Loading	632:638	Loading of 15 wt% ACB related to pure alginate	632:677	Loading of 15 wt% ACB related to pure alginate increased removal yield of alginate significantly.
33716132	3	45	from	effect	366:371	arg1	behavior					438:445	the methylene blue adsorption behavior	408:445	the methylene blue adsorption behavior of alginate	408:457	Here, the effect of activated carbon black (ACB) on the methylene blue adsorption behavior of alginate was examined.
33716132	6	46	theme	%	648:648	arg1	ACB					650:652	15 wt% ACB	643:652	15 wt% ACB related to pure alginate	643:677	Loading of 15 wt% ACB related to pure alginate increased removal yield of alginate significantly.
33716132	0	47	theme	activated	89:97	arg1	beads					116:120	activated carbon composite beads	89:120	activated carbon composite beads	89:120	Methylene blue contaminated water sanitization with alginate/compact discs waste-derived activated carbon composite beads: Adsorption studies.
33716132	7	48	theme	function	876:883	arg1	values					885:890	different error function values	860:890	different error function values	860:890	Isotherms and kinetic models in linear and non-linear forms were studied and the results were examined by comparing R2 along with different error function values to find the best fitting.
33716132	6	49	theme	15 wt	643:647	arg1	ACB					650:652	15 wt% ACB	643:652	15 wt% ACB related to pure alginate	643:677	Loading of 15 wt% ACB related to pure alginate increased removal yield of alginate significantly.
33716132	7	50	theme	kinetic	744:750	arg1	models					752:757	kinetic models	744:757	kinetic models	744:757	Isotherms and kinetic models in linear and non-linear forms were studied and the results were examined by comparing R2 along with different error function values to find the best fitting.
33716132	8	51	theme	non-linear	953:962	arg1	pseudo-second-order					964:982	non-linear pseudo-second-order	953:982	non-linear pseudo-second-order	953:982	The results were well matched with non-linear pseudo-second-order and linear Langmuir.
33716132	1	52	theme	different	170:178	arg1	adsorbents					180:189	different adsorbents	170:189	different adsorbents	170:189	The adsorption behavior of different adsorbents has been studied by various research works.
32193484	0	0	theme	goats	61:65	arg1	metabolomics					39:50	milk metabolomics	34:50	milk metabolomics	34:50	Milk yield, milk composition, and milk metabolomics of dairy goats intramammary-challenged with lipopolysaccharide under heat stress conditions.
32193484	0	0	theme	goats	61:65	arg1	yield					5:9	Milk yield	0:9	Milk yield	0:9	Milk yield, milk composition, and milk metabolomics of dairy goats intramammary-challenged with lipopolysaccharide under heat stress conditions.
32193484	0	0	theme	goats	61:65	arg1	composition					17:27	milk composition	12:27	milk composition	12:27	Milk yield, milk composition, and milk metabolomics of dairy goats intramammary-challenged with lipopolysaccharide under heat stress conditions.
32193484	3	1	theme	night	594:598	arg1	humidity					605:612	28 °C night; 40% humidity	588:612	28 °C night; 40% humidity	588:612	Changes in milk composition and milk metabolomics were evaluated after the administration of LPS in mammary glands of dairy goats under thermal-neutral (TN; n = 4; 15 to 20 °C; 40 to 45% humidity) or HS (n = 4; 35 °C day, 28 °C night; 40% humidity) conditions.
32193484	3	2	theme	mammary	466:472	arg1	glands					474:479	mammary glands	466:479	mammary glands of dairy goats under thermal-neutral (TN; n = 4; 15 to 20 °C; 40 to 45% humidity) or HS (n = 4; 35 °C day, 28 °C night; 40% humidity) conditions	466:624	Changes in milk composition and milk metabolomics were evaluated after the administration of LPS in mammary glands of dairy goats under thermal-neutral (TN; n = 4; 15 to 20 °C; 40 to 45% humidity) or HS (n = 4; 35 °C day, 28 °C night; 40% humidity) conditions.
32193484	6	3	theme	febrile	869:875	arg1	response					877:884	febrile response	869:884	febrile response that was detectable in TN goats, but was masked by elevated body temperature due to heat load in HS goats	869:990	Mammary treatment with LPS resulted in febrile response that was detectable in TN goats, but was masked by elevated body temperature due to heat load in HS goats.
32193484	0	4	theme	dairy	55:59	arg1	goats					61:65	dairy goats	55:65	dairy goats	55:65	Milk yield, milk composition, and milk metabolomics of dairy goats intramammary-challenged with lipopolysaccharide under heat stress conditions.
32193484	5	5	theme	milk	790:793	arg1	yield					795:799	milk yield	790:799	milk yield	790:799	Heat stress reduced feed intake and milk yield by 28 and 21%, respectively.
32193484	10	6	theme	milk	1507:1510	arg1	metabolomics					1512:1523	milk metabolomics	1507:1523	milk metabolomics	1507:1523	In conclusion, changes in milk somatic cells and milk metabolomics indicated that heat stress affected the mammary immune response to simulated infection, which could make dairy animals more vulnerable to mastitis.
32193484	3	7	dep	n = 4	570:574	arg1	day					583:585	35 °C day	577:585	35 °C day	577:585	Changes in milk composition and milk metabolomics were evaluated after the administration of LPS in mammary glands of dairy goats under thermal-neutral (TN; n = 4; 15 to 20 °C; 40 to 45% humidity) or HS (n = 4; 35 °C day, 28 °C night; 40% humidity) conditions.
32193484	3	7	dep	n = 4	570:574	arg1	humidity					605:612	28 °C night; 40% humidity	588:612	28 °C night; 40% humidity	588:612	Changes in milk composition and milk metabolomics were evaluated after the administration of LPS in mammary glands of dairy goats under thermal-neutral (TN; n = 4; 15 to 20 °C; 40 to 45% humidity) or HS (n = 4; 35 °C day, 28 °C night; 40% humidity) conditions.
32193484	6	8	theme	body	946:949	arg1	temperature					951:961	elevated body temperature	937:961	elevated body temperature due to heat load in HS goats	937:990	Mammary treatment with LPS resulted in febrile response that was detectable in TN goats, but was masked by elevated body temperature due to heat load in HS goats.
32193484	9	9	theme	putative	1347:1354	arg1	markers					1356:1362	putative markers	1347:1362	putative markers of inflammation with different pattern according to the ambient temperature (i.e. TN vs. HS)	1347:1455	Milk metabolomics revealed that citrate increased by HS, whereas choline, phosphocholine, N-acetylcarbohydrates, lactate, and ß-hydroxybutyrate could be considered as putative markers of inflammation with different pattern according to the ambient temperature (i.e. TN vs. HS).
32193484	9	9	theme	putative	1347:1354	arg1	phosphocholine					1254:1267	phosphocholine	1254:1267	phosphocholine	1254:1267	Milk metabolomics revealed that citrate increased by HS, whereas choline, phosphocholine, N-acetylcarbohydrates, lactate, and ß-hydroxybutyrate could be considered as putative markers of inflammation with different pattern according to the ambient temperature (i.e. TN vs. HS).
32193484	9	9	theme	putative	1347:1354	arg1	ß-hydroxybutyrate					1306:1322	ß-hydroxybutyrate	1306:1322	ß-hydroxybutyrate	1306:1322	Milk metabolomics revealed that citrate increased by HS, whereas choline, phosphocholine, N-acetylcarbohydrates, lactate, and ß-hydroxybutyrate could be considered as putative markers of inflammation with different pattern according to the ambient temperature (i.e. TN vs. HS).
32193484	9	9	theme	putative	1347:1354	arg1	N-acetylcarbohydrates					1270:1290	N-acetylcarbohydrates	1270:1290	N-acetylcarbohydrates	1270:1290	Milk metabolomics revealed that citrate increased by HS, whereas choline, phosphocholine, N-acetylcarbohydrates, lactate, and ß-hydroxybutyrate could be considered as putative markers of inflammation with different pattern according to the ambient temperature (i.e. TN vs. HS).
32193484	9	9	theme	putative	1347:1354	arg1	choline					1245:1251	choline	1245:1251	choline	1245:1251	Milk metabolomics revealed that citrate increased by HS, whereas choline, phosphocholine, N-acetylcarbohydrates, lactate, and ß-hydroxybutyrate could be considered as putative markers of inflammation with different pattern according to the ambient temperature (i.e. TN vs. HS).
32193484	9	9	theme	putative	1347:1354	arg1	lactate					1293:1299	lactate	1293:1299	lactate	1293:1299	Milk metabolomics revealed that citrate increased by HS, whereas choline, phosphocholine, N-acetylcarbohydrates, lactate, and ß-hydroxybutyrate could be considered as putative markers of inflammation with different pattern according to the ambient temperature (i.e. TN vs. HS).
32193484	6	10	from	detectable	895:904	arg1	goats					912:916	TN goats	909:916	TN goats	909:916	Mammary treatment with LPS resulted in febrile response that was detectable in TN goats, but was masked by elevated body temperature due to heat load in HS goats.
32193484	7	11	from	changes	1079:1085	arg1	goats					1093:1097	HS goats	1090:1097	HS goats	1090:1097	Additionally, LPS increased milk protein and decreased milk lactose, with more marked changes in HS goats.
32193484	9	12	theme	inflammation	1367:1378	arg1	markers					1356:1362	putative markers	1347:1362	putative markers of inflammation with different pattern according to the ambient temperature (i.e. TN vs. HS)	1347:1455	Milk metabolomics revealed that citrate increased by HS, whereas choline, phosphocholine, N-acetylcarbohydrates, lactate, and ß-hydroxybutyrate could be considered as putative markers of inflammation with different pattern according to the ambient temperature (i.e. TN vs. HS).
32193484	9	12	theme	inflammation	1367:1378	arg1	phosphocholine					1254:1267	phosphocholine	1254:1267	phosphocholine	1254:1267	Milk metabolomics revealed that citrate increased by HS, whereas choline, phosphocholine, N-acetylcarbohydrates, lactate, and ß-hydroxybutyrate could be considered as putative markers of inflammation with different pattern according to the ambient temperature (i.e. TN vs. HS).
32193484	9	12	theme	inflammation	1367:1378	arg1	ß-hydroxybutyrate					1306:1322	ß-hydroxybutyrate	1306:1322	ß-hydroxybutyrate	1306:1322	Milk metabolomics revealed that citrate increased by HS, whereas choline, phosphocholine, N-acetylcarbohydrates, lactate, and ß-hydroxybutyrate could be considered as putative markers of inflammation with different pattern according to the ambient temperature (i.e. TN vs. HS).
32193484	9	12	theme	inflammation	1367:1378	arg1	N-acetylcarbohydrates					1270:1290	N-acetylcarbohydrates	1270:1290	N-acetylcarbohydrates	1270:1290	Milk metabolomics revealed that citrate increased by HS, whereas choline, phosphocholine, N-acetylcarbohydrates, lactate, and ß-hydroxybutyrate could be considered as putative markers of inflammation with different pattern according to the ambient temperature (i.e. TN vs. HS).
32193484	9	12	theme	inflammation	1367:1378	arg1	choline					1245:1251	choline	1245:1251	choline	1245:1251	Milk metabolomics revealed that citrate increased by HS, whereas choline, phosphocholine, N-acetylcarbohydrates, lactate, and ß-hydroxybutyrate could be considered as putative markers of inflammation with different pattern according to the ambient temperature (i.e. TN vs. HS).
32193484	9	12	theme	inflammation	1367:1378	arg1	lactate					1293:1299	lactate	1293:1299	lactate	1293:1299	Milk metabolomics revealed that citrate increased by HS, whereas choline, phosphocholine, N-acetylcarbohydrates, lactate, and ß-hydroxybutyrate could be considered as putative markers of inflammation with different pattern according to the ambient temperature (i.e. TN vs. HS).
32193484	6	13	theme	TN	909:910	arg1	goats					912:916	TN goats	909:916	TN goats	909:916	Mammary treatment with LPS resulted in febrile response that was detectable in TN goats, but was masked by elevated body temperature due to heat load in HS goats.
32193484	7	14	theme	milk	1048:1051	arg1	lactose					1053:1059	milk lactose	1048:1059	milk lactose	1048:1059	Additionally, LPS increased milk protein and decreased milk lactose, with more marked changes in HS goats.
32193484	3	15	theme	%	603:603	arg1	humidity					605:612	28 °C night; 40% humidity	588:612	28 °C night; 40% humidity	588:612	Changes in milk composition and milk metabolomics were evaluated after the administration of LPS in mammary glands of dairy goats under thermal-neutral (TN; n = 4; 15 to 20 °C; 40 to 45% humidity) or HS (n = 4; 35 °C day, 28 °C night; 40% humidity) conditions.
32193484	10	16	theme	immune	1573:1578	arg1	response					1580:1587	the mammary immune response	1561:1587	the mammary immune response to simulated infection, which could make dairy animals more vulnerable to mastitis	1561:1670	In conclusion, changes in milk somatic cells and milk metabolomics indicated that heat stress affected the mammary immune response to simulated infection, which could make dairy animals more vulnerable to mastitis.
32193484	2	17	theme	immune	272:277	arg1	response					279:286	goat's mammary gland immune response	251:286	goat's mammary gland immune response to E. coli lipopolysaccharide (LPS)	251:322	The objective was to test whether goat's mammary gland immune response to E. coli lipopolysaccharide (LPS) could be conditioned by heat stress (HS).
32193484	8	18	theme	cells	1127:1131	arg1	recruitment					1104:1114	The recruitment	1100:1114	The recruitment of somatic cells in milk after LPS treatment	1100:1159	The recruitment of somatic cells in milk after LPS treatment was delayed by HS.
32193484	8	19	theme	somatic	1119:1125	arg1	cells					1127:1131	somatic cells	1119:1131	somatic cells	1119:1131	The recruitment of somatic cells in milk after LPS treatment was delayed by HS.
32193484	10	20	theme	somatic	1489:1495	arg1	cells					1497:1501	milk somatic cells	1484:1501	milk somatic cells	1484:1501	In conclusion, changes in milk somatic cells and milk metabolomics indicated that heat stress affected the mammary immune response to simulated infection, which could make dairy animals more vulnerable to mastitis.
32193484	2	21	theme	gland	266:270	arg1	response					279:286	goat's mammary gland immune response	251:286	goat's mammary gland immune response to E. coli lipopolysaccharide (LPS)	251:322	The objective was to test whether goat's mammary gland immune response to E. coli lipopolysaccharide (LPS) could be conditioned by heat stress (HS).
32193484	3	22	dep	20 °C	536:540	arg1	to					533:534	to	533:534	to	533:534	Changes in milk composition and milk metabolomics were evaluated after the administration of LPS in mammary glands of dairy goats under thermal-neutral (TN; n = 4; 15 to 20 °C; 40 to 45% humidity) or HS (n = 4; 35 °C day, 28 °C night; 40% humidity) conditions.
32193484	3	23	theme	milk	398:401	arg1	metabolomics					403:414	milk metabolomics	398:414	milk metabolomics	398:414	Changes in milk composition and milk metabolomics were evaluated after the administration of LPS in mammary glands of dairy goats under thermal-neutral (TN; n = 4; 15 to 20 °C; 40 to 45% humidity) or HS (n = 4; 35 °C day, 28 °C night; 40% humidity) conditions.
32193484	10	24	theme	milk	1484:1487	arg1	cells					1497:1501	milk somatic cells	1484:1501	milk somatic cells	1484:1501	In conclusion, changes in milk somatic cells and milk metabolomics indicated that heat stress affected the mammary immune response to simulated infection, which could make dairy animals more vulnerable to mastitis.
32193484	6	25	theme	heat	970:973	arg1	load					975:978	heat load	970:978	heat load in HS goats	970:990	Mammary treatment with LPS resulted in febrile response that was detectable in TN goats, but was masked by elevated body temperature due to heat load in HS goats.
32193484	2	26	theme	mammary	258:264	arg1	gland					266:270	goat's mammary gland	251:270	goat's mammary gland immune response to E. coli lipopolysaccharide (LPS)	251:322	The objective was to test whether goat's mammary gland immune response to E. coli lipopolysaccharide (LPS) could be conditioned by heat stress (HS).
32193484	6	27	theme	elevated	937:944	arg1	temperature					951:961	elevated body temperature	937:961	elevated body temperature due to heat load in HS goats	937:990	Mammary treatment with LPS resulted in febrile response that was detectable in TN goats, but was masked by elevated body temperature due to heat load in HS goats.
32193484	1	28	theme	major	174:178	arg1	stress					150:155	Heat stress	145:155	Heat stress	145:155	Heat stress and mastitis are major economic issues in dairy production.
32193484	1	28	theme	major	174:178	arg1	issues					189:194	major economic issues	174:194	major economic issues in dairy production	174:214	Heat stress and mastitis are major economic issues in dairy production.
32193484	1	28	theme	major	174:178	arg1	mastitis					161:168	mastitis	161:168	mastitis	161:168	Heat stress and mastitis are major economic issues in dairy production.
32193484	3	29	theme	goats	490:494	arg1	glands					474:479	mammary glands	466:479	mammary glands of dairy goats under thermal-neutral (TN; n = 4; 15 to 20 °C; 40 to 45% humidity) or HS (n = 4; 35 °C day, 28 °C night; 40% humidity) conditions	466:624	Changes in milk composition and milk metabolomics were evaluated after the administration of LPS in mammary glands of dairy goats under thermal-neutral (TN; n = 4; 15 to 20 °C; 40 to 45% humidity) or HS (n = 4; 35 °C day, 28 °C night; 40% humidity) conditions.
32193484	1	30	theme	economic	180:187	arg1	stress					150:155	Heat stress	145:155	Heat stress	145:155	Heat stress and mastitis are major economic issues in dairy production.
32193484	1	30	theme	economic	180:187	arg1	issues					189:194	major economic issues	174:194	major economic issues in dairy production	174:214	Heat stress and mastitis are major economic issues in dairy production.
32193484	1	30	theme	economic	180:187	arg1	mastitis					161:168	mastitis	161:168	mastitis	161:168	Heat stress and mastitis are major economic issues in dairy production.
32193484	0	31	theme	Milk	0:3	arg1	yield					5:9	Milk yield	0:9	Milk yield	0:9	Milk yield, milk composition, and milk metabolomics of dairy goats intramammary-challenged with lipopolysaccharide under heat stress conditions.
32193484	7	32	theme	marked	1072:1077	arg1	changes					1079:1085	more marked changes	1067:1085	more marked changes in HS goats	1067:1097	Additionally, LPS increased milk protein and decreased milk lactose, with more marked changes in HS goats.
32193484	0	33	theme	milk	12:15	arg1	composition					17:27	milk composition	12:27	milk composition	12:27	Milk yield, milk composition, and milk metabolomics of dairy goats intramammary-challenged with lipopolysaccharide under heat stress conditions.
32193484	0	34	theme	stress	126:131	arg1	conditions					133:142	heat stress conditions	121:142	heat stress conditions	121:142	Milk yield, milk composition, and milk metabolomics of dairy goats intramammary-challenged with lipopolysaccharide under heat stress conditions.
32193484	6	35	from	goats	912:916	arg1	detectable					895:904	detectable	895:904	detectable	895:904	Mammary treatment with LPS resulted in febrile response that was detectable in TN goats, but was masked by elevated body temperature due to heat load in HS goats.
32193484	2	36	theme	E.	291:292	arg1	LPS					319:321	LPS	319:321	LPS	319:321	The objective was to test whether goat's mammary gland immune response to E. coli lipopolysaccharide (LPS) could be conditioned by heat stress (HS).
32193484	2	36	theme	E.	291:292	arg1	lipopolysaccharide					299:316	lipopolysaccharide	299:316	E. coli lipopolysaccharide (LPS)	291:322	The objective was to test whether goat's mammary gland immune response to E. coli lipopolysaccharide (LPS) could be conditioned by heat stress (HS).
32193484	9	37	dep	TN	1446:1447	arg1	i.e.					1441:1444	i.e.	1441:1444	i.e.	1441:1444	Milk metabolomics revealed that citrate increased by HS, whereas choline, phosphocholine, N-acetylcarbohydrates, lactate, and ß-hydroxybutyrate could be considered as putative markers of inflammation with different pattern according to the ambient temperature (i.e. TN vs. HS).
32193484	6	38	theme	Mammary	830:836	arg1	treatment					838:846	Mammary treatment	830:846	Mammary treatment with LPS	830:855	Mammary treatment with LPS resulted in febrile response that was detectable in TN goats, but was masked by elevated body temperature due to heat load in HS goats.
32193484	0	39	theme	heat	121:124	arg1	conditions					133:142	heat stress conditions	121:142	heat stress conditions	121:142	Milk yield, milk composition, and milk metabolomics of dairy goats intramammary-challenged with lipopolysaccharide under heat stress conditions.
32193484	3	40	dep	TN	519:520	arg1	20 °C					536:540	20 °C	536:540	20 °C	536:540	Changes in milk composition and milk metabolomics were evaluated after the administration of LPS in mammary glands of dairy goats under thermal-neutral (TN; n = 4; 15 to 20 °C; 40 to 45% humidity) or HS (n = 4; 35 °C day, 28 °C night; 40% humidity) conditions.
32193484	3	40	dep	TN	519:520	arg1	n = 4					523:527	n = 4	523:527	n = 4	523:527	Changes in milk composition and milk metabolomics were evaluated after the administration of LPS in mammary glands of dairy goats under thermal-neutral (TN; n = 4; 15 to 20 °C; 40 to 45% humidity) or HS (n = 4; 35 °C day, 28 °C night; 40% humidity) conditions.
32193484	3	40	dep	TN	519:520	arg1	%					551:551	40 to 45%	543:551	TN; n = 4; 15 to 20 °C; 40 to 45% humidity	519:560	Changes in milk composition and milk metabolomics were evaluated after the administration of LPS in mammary glands of dairy goats under thermal-neutral (TN; n = 4; 15 to 20 °C; 40 to 45% humidity) or HS (n = 4; 35 °C day, 28 °C night; 40% humidity) conditions.
32193484	6	41	theme	HS	983:984	arg1	goats					986:990	HS goats	983:990	HS goats	983:990	Mammary treatment with LPS resulted in febrile response that was detectable in TN goats, but was masked by elevated body temperature due to heat load in HS goats.
32193484	1	42	theme	dairy	199:203	arg1	production					205:214	dairy production	199:214	dairy production	199:214	Heat stress and mastitis are major economic issues in dairy production.
32193484	8	43	theme	LPS	1147:1149	arg1	treatment					1151:1159	LPS treatment	1147:1159	LPS treatment	1147:1159	The recruitment of somatic cells in milk after LPS treatment was delayed by HS.
32193484	3	44	theme	HS	566:567	arg1	n = 4					570:574	n = 4	570:574	n = 4	570:574	Changes in milk composition and milk metabolomics were evaluated after the administration of LPS in mammary glands of dairy goats under thermal-neutral (TN; n = 4; 15 to 20 °C; 40 to 45% humidity) or HS (n = 4; 35 °C day, 28 °C night; 40% humidity) conditions.
32193484	3	44	theme	HS	566:567	arg1	conditions					615:624	thermal-neutral (TN; n = 4; 15 to 20 °C; 40 to 45% humidity) or HS (n = 4; 35 °C day, 28 °C night; 40% humidity) conditions	502:624	conditions	615:624	Changes in milk composition and milk metabolomics were evaluated after the administration of LPS in mammary glands of dairy goats under thermal-neutral (TN; n = 4; 15 to 20 °C; 40 to 45% humidity) or HS (n = 4; 35 °C day, 28 °C night; 40% humidity) conditions.
32193484	10	45	theme	dairy	1630:1634	arg1	animals					1636:1642	dairy animals	1630:1642	dairy animals	1630:1642	In conclusion, changes in milk somatic cells and milk metabolomics indicated that heat stress affected the mammary immune response to simulated infection, which could make dairy animals more vulnerable to mastitis.
32193484	3	46	dep	45	549:550	arg1	to					546:547	to	546:547	to	546:547	Changes in milk composition and milk metabolomics were evaluated after the administration of LPS in mammary glands of dairy goats under thermal-neutral (TN; n = 4; 15 to 20 °C; 40 to 45% humidity) or HS (n = 4; 35 °C day, 28 °C night; 40% humidity) conditions.
32193484	3	47	theme	milk	377:380	arg1	composition					382:392	milk composition	377:392	milk composition	377:392	Changes in milk composition and milk metabolomics were evaluated after the administration of LPS in mammary glands of dairy goats under thermal-neutral (TN; n = 4; 15 to 20 °C; 40 to 45% humidity) or HS (n = 4; 35 °C day, 28 °C night; 40% humidity) conditions.
32193484	3	48	from	Changes	366:372	arg1	composition					382:392	milk composition	377:392	milk composition	377:392	Changes in milk composition and milk metabolomics were evaluated after the administration of LPS in mammary glands of dairy goats under thermal-neutral (TN; n = 4; 15 to 20 °C; 40 to 45% humidity) or HS (n = 4; 35 °C day, 28 °C night; 40% humidity) conditions.
32193484	3	48	from	Changes	366:372	arg1	metabolomics					403:414	milk metabolomics	398:414	milk metabolomics	398:414	Changes in milk composition and milk metabolomics were evaluated after the administration of LPS in mammary glands of dairy goats under thermal-neutral (TN; n = 4; 15 to 20 °C; 40 to 45% humidity) or HS (n = 4; 35 °C day, 28 °C night; 40% humidity) conditions.
32193484	3	49	dep	%	551:551	arg1	humidity					553:560	humidity	553:560	TN; n = 4; 15 to 20 °C; 40 to 45% humidity	519:560	Changes in milk composition and milk metabolomics were evaluated after the administration of LPS in mammary glands of dairy goats under thermal-neutral (TN; n = 4; 15 to 20 °C; 40 to 45% humidity) or HS (n = 4; 35 °C day, 28 °C night; 40% humidity) conditions.
32193484	0	50	theme	milk	34:37	arg1	metabolomics					39:50	milk metabolomics	34:50	milk metabolomics	34:50	Milk yield, milk composition, and milk metabolomics of dairy goats intramammary-challenged with lipopolysaccharide under heat stress conditions.
32193484	5	51	theme	feed	774:777	arg1	intake					779:784	feed intake	774:784	feed intake	774:784	Heat stress reduced feed intake and milk yield by 28 and 21%, respectively.
32193484	9	52	dep	temperature	1428:1438	arg1	HS					1453:1454	HS	1453:1454	HS	1453:1454	Milk metabolomics revealed that citrate increased by HS, whereas choline, phosphocholine, N-acetylcarbohydrates, lactate, and ß-hydroxybutyrate could be considered as putative markers of inflammation with different pattern according to the ambient temperature (i.e. TN vs. HS).
32193484	9	52	dep	temperature	1428:1438	arg1	TN					1446:1447	TN	1446:1447	TN	1446:1447	Milk metabolomics revealed that citrate increased by HS, whereas choline, phosphocholine, N-acetylcarbohydrates, lactate, and ß-hydroxybutyrate could be considered as putative markers of inflammation with different pattern according to the ambient temperature (i.e. TN vs. HS).
32193484	4	53	theme	multivariate	714:725	arg1	analyses					727:734	multivariate analyses	714:734	multivariate analyses	714:734	Milk metabolomics were evaluated using 1H nuclear magnetic resonance spectroscopy, and multivariate analyses were carried out.
32193484	6	54	theme	due	963:965	arg1	temperature					951:961	elevated body temperature	937:961	elevated body temperature due to heat load in HS goats	937:990	Mammary treatment with LPS resulted in febrile response that was detectable in TN goats, but was masked by elevated body temperature due to heat load in HS goats.
32193484	3	55	theme	LPS	459:461	arg1	administration					441:454	the administration	437:454	the administration of LPS	437:461	Changes in milk composition and milk metabolomics were evaluated after the administration of LPS in mammary glands of dairy goats under thermal-neutral (TN; n = 4; 15 to 20 °C; 40 to 45% humidity) or HS (n = 4; 35 °C day, 28 °C night; 40% humidity) conditions.
32193484	10	56	theme	mammary	1565:1571	arg1	response					1580:1587	the mammary immune response	1561:1587	the mammary immune response to simulated infection, which could make dairy animals more vulnerable to mastitis	1561:1670	In conclusion, changes in milk somatic cells and milk metabolomics indicated that heat stress affected the mammary immune response to simulated infection, which could make dairy animals more vulnerable to mastitis.
32193484	10	57	from	changes	1473:1479	arg1	metabolomics					1512:1523	milk metabolomics	1507:1523	milk metabolomics	1507:1523	In conclusion, changes in milk somatic cells and milk metabolomics indicated that heat stress affected the mammary immune response to simulated infection, which could make dairy animals more vulnerable to mastitis.
32193484	10	57	from	changes	1473:1479	arg1	cells					1497:1501	milk somatic cells	1484:1501	milk somatic cells	1484:1501	In conclusion, changes in milk somatic cells and milk metabolomics indicated that heat stress affected the mammary immune response to simulated infection, which could make dairy animals more vulnerable to mastitis.
32193484	10	58	theme	simulated	1592:1600	arg1	infection					1602:1610	simulated infection	1592:1610	simulated infection	1592:1610	In conclusion, changes in milk somatic cells and milk metabolomics indicated that heat stress affected the mammary immune response to simulated infection, which could make dairy animals more vulnerable to mastitis.
32193484	1	59	theme	Heat	145:148	arg1	stress					150:155	Heat stress	145:155	Heat stress	145:155	Heat stress and mastitis are major economic issues in dairy production.
32193484	1	59	theme	Heat	145:148	arg1	issues					189:194	major economic issues	174:194	major economic issues in dairy production	174:214	Heat stress and mastitis are major economic issues in dairy production.
32193484	1	59	theme	Heat	145:148	arg1	mastitis					161:168	mastitis	161:168	mastitis	161:168	Heat stress and mastitis are major economic issues in dairy production.
32193484	4	60	theme	nuclear	669:675	arg1	resonance					686:694	1H nuclear magnetic resonance	666:694	1H nuclear magnetic resonance spectroscopy	666:707	Milk metabolomics were evaluated using 1H nuclear magnetic resonance spectroscopy, and multivariate analyses were carried out.
32193484	6	61	from	load	975:978	arg1	goats					986:990	HS goats	983:990	HS goats	983:990	Mammary treatment with LPS resulted in febrile response that was detectable in TN goats, but was masked by elevated body temperature due to heat load in HS goats.
32193484	2	62	dep	lipopolysaccharide	299:316	arg1	coli					294:297	E. coli lipopolysaccharide (LPS)	291:322	E. coli lipopolysaccharide (LPS)	291:322	The objective was to test whether goat's mammary gland immune response to E. coli lipopolysaccharide (LPS) could be conditioned by heat stress (HS).
32193484	4	63	theme	Milk	627:630	arg1	metabolomics					632:643	Milk metabolomics	627:643	Milk metabolomics	627:643	Milk metabolomics were evaluated using 1H nuclear magnetic resonance spectroscopy, and multivariate analyses were carried out.
32193484	10	64	theme	heat	1540:1543	arg1	stress					1545:1550	heat stress	1540:1550	heat stress	1540:1550	In conclusion, changes in milk somatic cells and milk metabolomics indicated that heat stress affected the mammary immune response to simulated infection, which could make dairy animals more vulnerable to mastitis.
32193484	6	65	with	treatment	838:846	arg1	LPS					853:855	LPS	853:855	LPS	853:855	Mammary treatment with LPS resulted in febrile response that was detectable in TN goats, but was masked by elevated body temperature due to heat load in HS goats.
32193484	4	66	theme	1H	666:667	arg1	resonance					686:694	1H nuclear magnetic resonance	666:694	1H nuclear magnetic resonance spectroscopy	666:707	Milk metabolomics were evaluated using 1H nuclear magnetic resonance spectroscopy, and multivariate analyses were carried out.
32193484	8	67	from	recruitment	1104:1114	arg1	milk					1136:1139	milk	1136:1139	milk after LPS treatment	1136:1159	The recruitment of somatic cells in milk after LPS treatment was delayed by HS.
32193484	7	68	theme	HS	1090:1091	arg1	goats					1093:1097	HS goats	1090:1097	HS goats	1090:1097	Additionally, LPS increased milk protein and decreased milk lactose, with more marked changes in HS goats.
32193484	3	69	dep	thermal-neutral	502:516	arg1	TN					519:520	TN	519:520	TN; n = 4; 15 to 20 °C; 40 to 45% humidity	519:560	Changes in milk composition and milk metabolomics were evaluated after the administration of LPS in mammary glands of dairy goats under thermal-neutral (TN; n = 4; 15 to 20 °C; 40 to 45% humidity) or HS (n = 4; 35 °C day, 28 °C night; 40% humidity) conditions.
32193484	4	70	theme	resonance	686:694	arg1	spectroscopy					696:707	1H nuclear magnetic resonance spectroscopy	666:707	1H nuclear magnetic resonance spectroscopy	666:707	Milk metabolomics were evaluated using 1H nuclear magnetic resonance spectroscopy, and multivariate analyses were carried out.
32193484	1	71	from	issues	189:194	arg1	production					205:214	dairy production	199:214	dairy production	199:214	Heat stress and mastitis are major economic issues in dairy production.
32193484	2	72	theme	heat	348:351	arg1	HS					361:362	HS	361:362	HS	361:362	The objective was to test whether goat's mammary gland immune response to E. coli lipopolysaccharide (LPS) could be conditioned by heat stress (HS).
32193484	2	72	theme	heat	348:351	arg1	stress					353:358	heat stress	348:358	heat stress (HS)	348:363	The objective was to test whether goat's mammary gland immune response to E. coli lipopolysaccharide (LPS) could be conditioned by heat stress (HS).
32193484	5	73	theme	Heat	754:757	arg1	stress					759:764	Heat stress	754:764	Heat stress	754:764	Heat stress reduced feed intake and milk yield by 28 and 21%, respectively.
32193484	9	74	theme	ambient	1420:1426	arg1	temperature					1428:1438	the ambient temperature	1416:1438	the ambient temperature (i.e. TN vs. HS)	1416:1455	Milk metabolomics revealed that citrate increased by HS, whereas choline, phosphocholine, N-acetylcarbohydrates, lactate, and ß-hydroxybutyrate could be considered as putative markers of inflammation with different pattern according to the ambient temperature (i.e. TN vs. HS).
32193484	9	75	with	inflammation	1367:1378	arg1	pattern					1395:1401	different pattern	1385:1401	different pattern according to the ambient temperature (i.e. TN vs. HS)	1385:1455	Milk metabolomics revealed that citrate increased by HS, whereas choline, phosphocholine, N-acetylcarbohydrates, lactate, and ß-hydroxybutyrate could be considered as putative markers of inflammation with different pattern according to the ambient temperature (i.e. TN vs. HS).
32193484	4	76	theme	magnetic	677:684	arg1	resonance					686:694	1H nuclear magnetic resonance	666:694	1H nuclear magnetic resonance spectroscopy	666:707	Milk metabolomics were evaluated using 1H nuclear magnetic resonance spectroscopy, and multivariate analyses were carried out.
32193484	9	77	theme	different	1385:1393	arg1	pattern					1395:1401	different pattern	1385:1401	different pattern according to the ambient temperature (i.e. TN vs. HS)	1385:1455	Milk metabolomics revealed that citrate increased by HS, whereas choline, phosphocholine, N-acetylcarbohydrates, lactate, and ß-hydroxybutyrate could be considered as putative markers of inflammation with different pattern according to the ambient temperature (i.e. TN vs. HS).
32193484	3	78	theme	dairy	484:488	arg1	goats					490:494	dairy goats	484:494	dairy goats under thermal-neutral (TN; n = 4; 15 to 20 °C; 40 to 45% humidity) or HS (n = 4; 35 °C day, 28 °C night; 40% humidity) conditions	484:624	Changes in milk composition and milk metabolomics were evaluated after the administration of LPS in mammary glands of dairy goats under thermal-neutral (TN; n = 4; 15 to 20 °C; 40 to 45% humidity) or HS (n = 4; 35 °C day, 28 °C night; 40% humidity) conditions.
32193484	7	79	theme	milk	1021:1024	arg1	protein					1026:1032	milk protein	1021:1032	milk protein	1021:1032	Additionally, LPS increased milk protein and decreased milk lactose, with more marked changes in HS goats.
32193484	9	80	theme	Milk	1180:1183	arg1	metabolomics					1185:1196	Milk metabolomics	1180:1196	Milk metabolomics	1180:1196	Milk metabolomics revealed that citrate increased by HS, whereas choline, phosphocholine, N-acetylcarbohydrates, lactate, and ß-hydroxybutyrate could be considered as putative markers of inflammation with different pattern according to the ambient temperature (i.e. TN vs. HS).
34591932	5	0	theme	in	953:954	arg1	digestibility					970:982	the in vitro ruminal digestibility	949:982	the in vitro ruminal digestibility	949:982	The enhancement in the in vitro ruminal digestibility was negatively correlated with the lignin content in the OPEFB.
34591932	3	1	theme	scanning	666:673	arg1	microscopy					684:693	scanning electron microscopy	666:693	scanning electron microscopy	666:693	The findings were corroborated using the physical examination of the OPEFB by scanning electron microscopy.
34591932	4	2	theme	organic	806:812	arg1	matter					823:828	organic (69.78%) matter	806:828	organic (69.78%) matter	806:828	Moreover, the OPEFB pre-treated for 12 weeks has shown the highest in vitro digestibility of dry (77.20%) and organic (69.78%) matter, where they were enhanced by 104.07 and 96.29%, respectively, as compared to the untreated control.
34591932	1	3	theme	bunch	246:250	arg1	digestibility					208:220	in vitro rumen digestibility	193:220	in vitro rumen digestibility	193:220	The changes in lignocellulosic biomass composition and in vitro rumen digestibility of oil palm empty fruit bunch (OPEFB) after pre-treatment with the fungus Ganoderma lucidum were evaluated.
34591932	1	3	theme	bunch	246:250	arg1	composition					177:187	lignocellulosic biomass composition	153:187	lignocellulosic biomass composition	153:187	The changes in lignocellulosic biomass composition and in vitro rumen digestibility of oil palm empty fruit bunch (OPEFB) after pre-treatment with the fungus Ganoderma lucidum were evaluated.
34591932	0	4	theme	bunch	65:69	arg1	digestibility					27:39	in vitro ruminal digestibility	10:39	in vitro ruminal digestibility of oil palm empty fruit bunch	10:69	Enhancing in vitro ruminal digestibility of oil palm empty fruit bunch by biological pre-treatment with Ganoderma lucidum fungal culture.
34591932	1	5	theme	lignocellulosic	153:167	arg1	composition					177:187	lignocellulosic biomass composition	153:187	lignocellulosic biomass composition	153:187	The changes in lignocellulosic biomass composition and in vitro rumen digestibility of oil palm empty fruit bunch (OPEFB) after pre-treatment with the fungus Ganoderma lucidum were evaluated.
34591932	5	6	dep	in	953:954	arg1	vitro					956:960	vitro	956:960	vitro	956:960	The enhancement in the in vitro ruminal digestibility was negatively correlated with the lignin content in the OPEFB.
34591932	3	7	theme	OPEFB	657:661	arg1	examination					638:648	the physical examination	625:648	the physical examination of the OPEFB by scanning electron microscopy	625:693	The findings were corroborated using the physical examination of the OPEFB by scanning electron microscopy.
34591932	0	8	theme	biological	74:83	arg1	pre-treatment					85:97	biological pre-treatment	74:97	biological pre-treatment with Ganoderma lucidum fungal culture	74:135	Enhancing in vitro ruminal digestibility of oil palm empty fruit bunch by biological pre-treatment with Ganoderma lucidum fungal culture.
34591932	5	9	from	enhancement	934:944	arg1	digestibility					970:982	the in vitro ruminal digestibility	949:982	the in vitro ruminal digestibility	949:982	The enhancement in the in vitro ruminal digestibility was negatively correlated with the lignin content in the OPEFB.
34591932	0	10	theme	Ganoderma	104:112	arg1	lucidum					114:120	Ganoderma lucidum	104:120	Ganoderma lucidum fungal culture	104:135	Enhancing in vitro ruminal digestibility of oil palm empty fruit bunch by biological pre-treatment with Ganoderma lucidum fungal culture.
34591932	1	11	from	changes	142:148	arg1	digestibility					208:220	in vitro rumen digestibility	193:220	in vitro rumen digestibility	193:220	The changes in lignocellulosic biomass composition and in vitro rumen digestibility of oil palm empty fruit bunch (OPEFB) after pre-treatment with the fungus Ganoderma lucidum were evaluated.
34591932	1	11	from	changes	142:148	arg1	composition					177:187	lignocellulosic biomass composition	153:187	lignocellulosic biomass composition	153:187	The changes in lignocellulosic biomass composition and in vitro rumen digestibility of oil palm empty fruit bunch (OPEFB) after pre-treatment with the fungus Ganoderma lucidum were evaluated.
34591932	6	12	theme	delignified	1072:1082	arg1	OPEFB					1084:1088	biologically delignified OPEFB	1059:1088	biologically delignified OPEFB with G. lucidum fungal culture pre-treatment	1059:1133	Therefore, biologically delignified OPEFB with G. lucidum fungal culture pre-treatment have the potential to be utilized as one of the ingredients for the development of a novel ruminant forage.
34591932	1	13	theme	biomass	169:175	arg1	composition					177:187	lignocellulosic biomass composition	153:187	lignocellulosic biomass composition	153:187	The changes in lignocellulosic biomass composition and in vitro rumen digestibility of oil palm empty fruit bunch (OPEFB) after pre-treatment with the fungus Ganoderma lucidum were evaluated.
34591932	5	14	from	content	1026:1032	arg1	OPEFB					1041:1045	the OPEFB	1037:1045	the OPEFB	1037:1045	The enhancement in the in vitro ruminal digestibility was negatively correlated with the lignin content in the OPEFB.
34591932	3	15	theme	electron	675:682	arg1	microscopy					684:693	scanning electron microscopy	666:693	scanning electron microscopy	666:693	The findings were corroborated using the physical examination of the OPEFB by scanning electron microscopy.
34591932	4	16	theme	untreated	911:919	arg1	control					921:927	the untreated control	907:927	the untreated control	907:927	Moreover, the OPEFB pre-treated for 12 weeks has shown the highest in vitro digestibility of dry (77.20%) and organic (69.78%) matter, where they were enhanced by 104.07 and 96.29%, respectively, as compared to the untreated control.
34591932	4	17	dep	highest	755:761	arg1	vitro					766:770	vitro	766:770	vitro	766:770	Moreover, the OPEFB pre-treated for 12 weeks has shown the highest in vitro digestibility of dry (77.20%) and organic (69.78%) matter, where they were enhanced by 104.07 and 96.29%, respectively, as compared to the untreated control.
34591932	0	18	theme	in	10:11	arg1	digestibility					27:39	in vitro ruminal digestibility	10:39	in vitro ruminal digestibility of oil palm empty fruit bunch	10:69	Enhancing in vitro ruminal digestibility of oil palm empty fruit bunch by biological pre-treatment with Ganoderma lucidum fungal culture.
34591932	2	19	theme	period	580:585	arg1	end					558:560	the end	554:560	the end of the incubation period	554:585	The results demonstrated that the pre-treatment for 2-12 weeks has gradually degraded the OPEFB in a time-dependent manner; whereby lignin, cellulose, and hemicellulose were respectively degraded by 41.0, 20.5, and 26.7% at the end of the incubation period.
34591932	2	20	theme	time-dependent	431:444	arg1	manner					446:451	a time-dependent manner	429:451	a time-dependent manner	429:451	The results demonstrated that the pre-treatment for 2-12 weeks has gradually degraded the OPEFB in a time-dependent manner; whereby lignin, cellulose, and hemicellulose were respectively degraded by 41.0, 20.5, and 26.7% at the end of the incubation period.
34591932	6	21	theme	lucidum	1098:1104	arg1	pre-treatment					1121:1133	G. lucidum fungal culture pre-treatment	1095:1133	G. lucidum fungal culture pre-treatment	1095:1133	Therefore, biologically delignified OPEFB with G. lucidum fungal culture pre-treatment have the potential to be utilized as one of the ingredients for the development of a novel ruminant forage.
34591932	2	22	theme	incubation	569:578	arg1	period					580:585	the incubation period	565:585	the incubation period	565:585	The results demonstrated that the pre-treatment for 2-12 weeks has gradually degraded the OPEFB in a time-dependent manner; whereby lignin, cellulose, and hemicellulose were respectively degraded by 41.0, 20.5, and 26.7% at the end of the incubation period.
34591932	0	23	with	pre-treatment	85:97	arg1	culture					129:135	Ganoderma lucidum fungal culture	104:135	Ganoderma lucidum fungal culture	104:135	Enhancing in vitro ruminal digestibility of oil palm empty fruit bunch by biological pre-treatment with Ganoderma lucidum fungal culture.
34591932	6	24	theme	ruminant	1226:1233	arg1	development					1203:1213	the development	1199:1213	the development of a novel ruminant forage	1199:1240	Therefore, biologically delignified OPEFB with G. lucidum fungal culture pre-treatment have the potential to be utilized as one of the ingredients for the development of a novel ruminant forage.
34591932	1	25	theme	in	193:194	arg1	digestibility					208:220	in vitro rumen digestibility	193:220	in vitro rumen digestibility	193:220	The changes in lignocellulosic biomass composition and in vitro rumen digestibility of oil palm empty fruit bunch (OPEFB) after pre-treatment with the fungus Ganoderma lucidum were evaluated.
34591932	0	26	theme	ruminal	19:25	arg1	digestibility					27:39	in vitro ruminal digestibility	10:39	in vitro ruminal digestibility of oil palm empty fruit bunch	10:69	Enhancing in vitro ruminal digestibility of oil palm empty fruit bunch by biological pre-treatment with Ganoderma lucidum fungal culture.
34591932	6	27	contain	have	1135:1138	arg2	potential					1144:1152	the potential to be utilized as one of the ingredients for the development of a novel ruminant forage	1140:1240	the potential to be utilized as one of the ingredients for the development of a novel ruminant forage	1140:1240	Therefore, biologically delignified OPEFB with G. lucidum fungal culture pre-treatment have the potential to be utilized as one of the ingredients for the development of a novel ruminant forage.
34591932	6	27	contain	have	1135:1138	arg1	OPEFB					1084:1088	biologically delignified OPEFB	1059:1088	biologically delignified OPEFB with G. lucidum fungal culture pre-treatment	1059:1133	Therefore, biologically delignified OPEFB with G. lucidum fungal culture pre-treatment have the potential to be utilized as one of the ingredients for the development of a novel ruminant forage.
34591932	0	28	theme	fungal	122:127	arg1	culture					129:135	Ganoderma lucidum fungal culture	104:135	Ganoderma lucidum fungal culture	104:135	Enhancing in vitro ruminal digestibility of oil palm empty fruit bunch by biological pre-treatment with Ganoderma lucidum fungal culture.
34591932	1	29	with	pre-treatment	266:278	arg1	lucidum					306:312	the fungus Ganoderma lucidum	285:312	the fungus Ganoderma lucidum	285:312	The changes in lignocellulosic biomass composition and in vitro rumen digestibility of oil palm empty fruit bunch (OPEFB) after pre-treatment with the fungus Ganoderma lucidum were evaluated.
34591932	0	30	theme	lucidum	114:120	arg1	culture					129:135	Ganoderma lucidum fungal culture	104:135	Ganoderma lucidum fungal culture	104:135	Enhancing in vitro ruminal digestibility of oil palm empty fruit bunch by biological pre-treatment with Ganoderma lucidum fungal culture.
34591932	1	31	theme	rumen	202:206	arg1	digestibility					208:220	in vitro rumen digestibility	193:220	in vitro rumen digestibility	193:220	The changes in lignocellulosic biomass composition and in vitro rumen digestibility of oil palm empty fruit bunch (OPEFB) after pre-treatment with the fungus Ganoderma lucidum were evaluated.
34591932	6	32	with	OPEFB	1084:1088	arg1	pre-treatment					1121:1133	G. lucidum fungal culture pre-treatment	1095:1133	G. lucidum fungal culture pre-treatment	1095:1133	Therefore, biologically delignified OPEFB with G. lucidum fungal culture pre-treatment have the potential to be utilized as one of the ingredients for the development of a novel ruminant forage.
34591932	6	33	theme	novel	1220:1224	arg1	ruminant					1226:1233	a novel ruminant	1218:1233	a novel ruminant forage	1218:1240	Therefore, biologically delignified OPEFB with G. lucidum fungal culture pre-treatment have the potential to be utilized as one of the ingredients for the development of a novel ruminant forage.
34591932	5	34	theme	ruminal	962:968	arg1	digestibility					970:982	the in vitro ruminal digestibility	949:982	the in vitro ruminal digestibility	949:982	The enhancement in the in vitro ruminal digestibility was negatively correlated with the lignin content in the OPEFB.
34591932	6	35	theme	ingredients	1183:1193	arg1	one					1172:1174	one	1172:1174	one	1172:1174	Therefore, biologically delignified OPEFB with G. lucidum fungal culture pre-treatment have the potential to be utilized as one of the ingredients for the development of a novel ruminant forage.
34591932	6	35	theme	ingredients	1183:1193	arg1	ingredients					1183:1193	the ingredients	1179:1193	the ingredients for the development of a novel ruminant forage	1179:1240	Therefore, biologically delignified OPEFB with G. lucidum fungal culture pre-treatment have the potential to be utilized as one of the ingredients for the development of a novel ruminant forage.
34591932	1	36	theme	fungus	289:294	arg1	lucidum					306:312	the fungus Ganoderma lucidum	285:312	the fungus Ganoderma lucidum	285:312	The changes in lignocellulosic biomass composition and in vitro rumen digestibility of oil palm empty fruit bunch (OPEFB) after pre-treatment with the fungus Ganoderma lucidum were evaluated.
34591932	1	37	dep	in	193:194	arg1	vitro					196:200	vitro	196:200	vitro	196:200	The changes in lignocellulosic biomass composition and in vitro rumen digestibility of oil palm empty fruit bunch (OPEFB) after pre-treatment with the fungus Ganoderma lucidum were evaluated.
34591932	0	38	theme	palm	48:51	arg1	bunch					65:69	oil palm empty fruit bunch	44:69	oil palm empty fruit bunch	44:69	Enhancing in vitro ruminal digestibility of oil palm empty fruit bunch by biological pre-treatment with Ganoderma lucidum fungal culture.
34591932	1	39	theme	Ganoderma	296:304	arg1	lucidum					306:312	the fungus Ganoderma lucidum	285:312	the fungus Ganoderma lucidum	285:312	The changes in lignocellulosic biomass composition and in vitro rumen digestibility of oil palm empty fruit bunch (OPEFB) after pre-treatment with the fungus Ganoderma lucidum were evaluated.
34591932	4	40	theme	dry	789:791	arg1	digestibility					772:784	the highest in vitro digestibility	751:784	the highest in vitro digestibility of dry (77.20%) and organic (69.78%) matter	751:828	Moreover, the OPEFB pre-treated for 12 weeks has shown the highest in vitro digestibility of dry (77.20%) and organic (69.78%) matter, where they were enhanced by 104.07 and 96.29%, respectively, as compared to the untreated control.
34591932	6	41	dep	ruminant	1226:1233	arg1	forage					1235:1240	forage	1235:1240	forage	1235:1240	Therefore, biologically delignified OPEFB with G. lucidum fungal culture pre-treatment have the potential to be utilized as one of the ingredients for the development of a novel ruminant forage.
34591932	0	42	theme	oil	44:46	arg1	bunch					65:69	oil palm empty fruit bunch	44:69	oil palm empty fruit bunch	44:69	Enhancing in vitro ruminal digestibility of oil palm empty fruit bunch by biological pre-treatment with Ganoderma lucidum fungal culture.
34591932	4	43	dep	organic	806:812	arg1	%					820:820	69.78%	815:820	69.78%	815:820	Moreover, the OPEFB pre-treated for 12 weeks has shown the highest in vitro digestibility of dry (77.20%) and organic (69.78%) matter, where they were enhanced by 104.07 and 96.29%, respectively, as compared to the untreated control.
34591932	6	44	theme	G.	1095:1096	arg1	lucidum					1098:1104	G. lucidum	1095:1104	G. lucidum fungal culture pre-treatment	1095:1133	Therefore, biologically delignified OPEFB with G. lucidum fungal culture pre-treatment have the potential to be utilized as one of the ingredients for the development of a novel ruminant forage.
34591932	6	45	theme	culture	1113:1119	arg1	pre-treatment					1121:1133	G. lucidum fungal culture pre-treatment	1095:1133	G. lucidum fungal culture pre-treatment	1095:1133	Therefore, biologically delignified OPEFB with G. lucidum fungal culture pre-treatment have the potential to be utilized as one of the ingredients for the development of a novel ruminant forage.
34591932	1	46	theme	oil	225:227	arg1	OPEFB					253:257	OPEFB	253:257	OPEFB	253:257	The changes in lignocellulosic biomass composition and in vitro rumen digestibility of oil palm empty fruit bunch (OPEFB) after pre-treatment with the fungus Ganoderma lucidum were evaluated.
34591932	1	46	theme	oil	225:227	arg1	bunch					246:250	oil palm empty fruit bunch	225:250	oil palm empty fruit bunch (OPEFB)	225:258	The changes in lignocellulosic biomass composition and in vitro rumen digestibility of oil palm empty fruit bunch (OPEFB) after pre-treatment with the fungus Ganoderma lucidum were evaluated.
34591932	4	47	theme	matter	823:828	arg1	digestibility					772:784	the highest in vitro digestibility	751:784	the highest in vitro digestibility of dry (77.20%) and organic (69.78%) matter	751:828	Moreover, the OPEFB pre-treated for 12 weeks has shown the highest in vitro digestibility of dry (77.20%) and organic (69.78%) matter, where they were enhanced by 104.07 and 96.29%, respectively, as compared to the untreated control.
34591932	6	48	theme	fungal	1106:1111	arg1	pre-treatment					1121:1133	G. lucidum fungal culture pre-treatment	1095:1133	G. lucidum fungal culture pre-treatment	1095:1133	Therefore, biologically delignified OPEFB with G. lucidum fungal culture pre-treatment have the potential to be utilized as one of the ingredients for the development of a novel ruminant forage.
34591932	1	49	theme	palm	229:232	arg1	OPEFB					253:257	OPEFB	253:257	OPEFB	253:257	The changes in lignocellulosic biomass composition and in vitro rumen digestibility of oil palm empty fruit bunch (OPEFB) after pre-treatment with the fungus Ganoderma lucidum were evaluated.
34591932	1	49	theme	palm	229:232	arg1	bunch					246:250	oil palm empty fruit bunch	225:250	oil palm empty fruit bunch (OPEFB)	225:258	The changes in lignocellulosic biomass composition and in vitro rumen digestibility of oil palm empty fruit bunch (OPEFB) after pre-treatment with the fungus Ganoderma lucidum were evaluated.
34591932	0	50	theme	fruit	59:63	arg1	bunch					65:69	oil palm empty fruit bunch	44:69	oil palm empty fruit bunch	44:69	Enhancing in vitro ruminal digestibility of oil palm empty fruit bunch by biological pre-treatment with Ganoderma lucidum fungal culture.
34591932	3	51	theme	physical	629:636	arg1	examination					638:648	the physical examination	625:648	the physical examination of the OPEFB by scanning electron microscopy	625:693	The findings were corroborated using the physical examination of the OPEFB by scanning electron microscopy.
34591932	0	52	dep	in	10:11	arg1	vitro					13:17	vitro	13:17	vitro	13:17	Enhancing in vitro ruminal digestibility of oil palm empty fruit bunch by biological pre-treatment with Ganoderma lucidum fungal culture.
34591932	1	53	theme	empty	234:238	arg1	OPEFB					253:257	OPEFB	253:257	OPEFB	253:257	The changes in lignocellulosic biomass composition and in vitro rumen digestibility of oil palm empty fruit bunch (OPEFB) after pre-treatment with the fungus Ganoderma lucidum were evaluated.
34591932	1	53	theme	empty	234:238	arg1	bunch					246:250	oil palm empty fruit bunch	225:250	oil palm empty fruit bunch (OPEFB)	225:258	The changes in lignocellulosic biomass composition and in vitro rumen digestibility of oil palm empty fruit bunch (OPEFB) after pre-treatment with the fungus Ganoderma lucidum were evaluated.
34591932	0	54	theme	empty	53:57	arg1	bunch					65:69	oil palm empty fruit bunch	44:69	oil palm empty fruit bunch	44:69	Enhancing in vitro ruminal digestibility of oil palm empty fruit bunch by biological pre-treatment with Ganoderma lucidum fungal culture.
34591932	5	55	theme	lignin	1019:1024	arg1	content					1026:1032	the lignin content	1015:1032	the lignin content in the OPEFB	1015:1045	The enhancement in the in vitro ruminal digestibility was negatively correlated with the lignin content in the OPEFB.
34591932	4	56	theme	highest	755:761	arg1	digestibility					772:784	the highest in vitro digestibility	751:784	the highest in vitro digestibility of dry (77.20%) and organic (69.78%) matter	751:828	Moreover, the OPEFB pre-treated for 12 weeks has shown the highest in vitro digestibility of dry (77.20%) and organic (69.78%) matter, where they were enhanced by 104.07 and 96.29%, respectively, as compared to the untreated control.
34591932	2	57	from	OPEFB	420:424	arg1	manner					446:451	a time-dependent manner	429:451	a time-dependent manner	429:451	The results demonstrated that the pre-treatment for 2-12 weeks has gradually degraded the OPEFB in a time-dependent manner; whereby lignin, cellulose, and hemicellulose were respectively degraded by 41.0, 20.5, and 26.7% at the end of the incubation period.
34591932	1	58	theme	fruit	240:244	arg1	OPEFB					253:257	OPEFB	253:257	OPEFB	253:257	The changes in lignocellulosic biomass composition and in vitro rumen digestibility of oil palm empty fruit bunch (OPEFB) after pre-treatment with the fungus Ganoderma lucidum were evaluated.
34591932	1	58	theme	fruit	240:244	arg1	bunch					246:250	oil palm empty fruit bunch	225:250	oil palm empty fruit bunch (OPEFB)	225:258	The changes in lignocellulosic biomass composition and in vitro rumen digestibility of oil palm empty fruit bunch (OPEFB) after pre-treatment with the fungus Ganoderma lucidum were evaluated.
33723988	6	0	theme	gauge	1071:1075	arg1	factor					1077:1082	gauge factor	1071:1082	gauge factor of 5.9	1071:1089	The obtained DN composite hydrogels possess good biocompatibility, stretchability (436%), fracture strength (41.0 KPa), self-healing property (healing efficiency of 95%), and high tensile strain sensitivity (gauge factor of 5.9).
33723988	4	1	theme	multifunctional	603:617	arg1	hydrogels					622:630	multifunctional DN hydrogels	603:630	multifunctional DN hydrogels	603:630	Here, multifunctional DN hydrogels are prepared via a combination of host-guest interaction of β-cyclodextrin and ferrocene with dynamic borate ester bonds of poly(vinyl alcohol) and borax.
33723988	10	2	theme	strain	1496:1501	arg1	sensors					1503:1509	flexible strain sensors	1487:1509	flexible strain sensors	1487:1509	This work exhibits new ideas and approaches to develop multifunctional self-healing hydrogels for constructing flexible strain sensors.
33723988	2	3	theme	good	366:369	arg1	properties					426:435	The good self-healing, biocompatible, sensitive and stretchable properties	362:435	The good self-healing, biocompatible, sensitive and stretchable properties	362:435	The good self-healing, biocompatible, sensitive and stretchable properties are the focus of hydrogel-based flexible strain sensors.
33723988	2	3	theme	good	366:369	arg1	focus					445:449	the focus	441:449	the focus of hydrogel-based flexible strain sensors	441:491	The good self-healing, biocompatible, sensitive and stretchable properties are the focus of hydrogel-based flexible strain sensors.
33723988	1	4	theme	early	277:281	arg1	diagnosis					291:299	early disease diagnosis	277:299	early disease diagnosis	277:299	Hydrogel-based flexible strain sensors have shown great potential in body movement tracking, early disease diagnosis, noninvasive treatment, electronic skins, and soft robotics.
33723988	4	5	theme	β-cyclodextrin	692:705	arg1	interaction					677:687	host-guest interaction	666:687	host-guest interaction of β-cyclodextrin and ferrocene with dynamic borate ester bonds of poly(vinyl alcohol) and borax	666:784	Here, multifunctional DN hydrogels are prepared via a combination of host-guest interaction of β-cyclodextrin and ferrocene with dynamic borate ester bonds of poly(vinyl alcohol) and borax.
33723988	0	6	theme	Multifunctional	0:14	arg1	Hydrogels					42:50	Multifunctional Self-Healing Dual Network Hydrogels	0:50	Multifunctional Self-Healing Dual Network Hydrogels	0:50	Multifunctional Self-Healing Dual Network Hydrogels Constructed via Host-Guest Interaction and Dynamic Covalent Bond as Wearable Strain Sensors for Monitoring Human and Organ Motions.
33723988	1	7	theme	flexible	199:206	arg1	sensors					215:221	Hydrogel-based flexible strain sensors	184:221	Hydrogel-based flexible strain sensors	184:221	Hydrogel-based flexible strain sensors have shown great potential in body movement tracking, early disease diagnosis, noninvasive treatment, electronic skins, and soft robotics.
33723988	5	8	with	hydrogels	829:837	arg1	conductivity					849:860	good conductivity	844:860	good conductivity	844:860	Carbon nanotubes are used to endow the DN hydrogels with good conductivity.
33723988	8	9	theme	healed	1210:1215	arg1	hydrogels					1217:1225	the healed hydrogels	1206:1225	the healed hydrogels	1206:1225	After cutting, the healed hydrogels also can monitor human motions and have good stability.
33723988	6	10	contain	possess	899:905	arg2	biocompatibility					912:927	good biocompatibility	907:927	good biocompatibility	907:927	The obtained DN composite hydrogels possess good biocompatibility, stretchability (436%), fracture strength (41.0 KPa), self-healing property (healing efficiency of 95%), and high tensile strain sensitivity (gauge factor of 5.9).
33723988	6	10	contain	possess	899:905	arg2	sensitivity					1058:1068	high tensile strain sensitivity	1038:1068	high tensile strain sensitivity (gauge factor of 5.9)	1038:1090	The obtained DN composite hydrogels possess good biocompatibility, stretchability (436%), fracture strength (41.0 KPa), self-healing property (healing efficiency of 95%), and high tensile strain sensitivity (gauge factor of 5.9).
33723988	6	10	contain	possess	899:905	arg2	strength					962:969	fracture strength	953:969	fracture strength (41.0 KPa)	953:980	The obtained DN composite hydrogels possess good biocompatibility, stretchability (436%), fracture strength (41.0 KPa), self-healing property (healing efficiency of 95%), and high tensile strain sensitivity (gauge factor of 5.9).
33723988	6	10	contain	possess	899:905	arg2	stretchability					930:943	stretchability	930:943	stretchability (436%)	930:950	The obtained DN composite hydrogels possess good biocompatibility, stretchability (436%), fracture strength (41.0 KPa), self-healing property (healing efficiency of 95%), and high tensile strain sensitivity (gauge factor of 5.9).
33723988	6	10	contain	possess	899:905	arg2	%					949:949	436%	946:949	436%	946:949	The obtained DN composite hydrogels possess good biocompatibility, stretchability (436%), fracture strength (41.0 KPa), self-healing property (healing efficiency of 95%), and high tensile strain sensitivity (gauge factor of 5.9).
33723988	6	10	contain	possess	899:905	arg2	property					996:1003	self-healing property	983:1003	self-healing property (healing efficiency of 95%)	983:1031	The obtained DN composite hydrogels possess good biocompatibility, stretchability (436%), fracture strength (41.0 KPa), self-healing property (healing efficiency of 95%), and high tensile strain sensitivity (gauge factor of 5.9).
33723988	6	10	contain	possess	899:905	arg2	%					1030:1030	healing efficiency of 95%	1006:1030	healing efficiency of 95%	1006:1030	The obtained DN composite hydrogels possess good biocompatibility, stretchability (436%), fracture strength (41.0 KPa), self-healing property (healing efficiency of 95%), and high tensile strain sensitivity (gauge factor of 5.9).
33723988	6	10	contain	possess	899:905	arg2	KPa					977:979	41.0 KPa	972:979	41.0 KPa	972:979	The obtained DN composite hydrogels possess good biocompatibility, stretchability (436%), fracture strength (41.0 KPa), self-healing property (healing efficiency of 95%), and high tensile strain sensitivity (gauge factor of 5.9).
33723988	6	10	contain	possess	899:905	arg1	hydrogels					889:897	The obtained DN composite hydrogels	863:897	The obtained DN composite hydrogels	863:897	The obtained DN composite hydrogels possess good biocompatibility, stretchability (436%), fracture strength (41.0 KPa), self-healing property (healing efficiency of 95%), and high tensile strain sensitivity (gauge factor of 5.9).
33723988	4	11	theme	interaction	677:687	arg1	combination					651:661	a combination	649:661	a combination of host-guest interaction of β-cyclodextrin and ferrocene with dynamic borate ester bonds of poly(vinyl alcohol) and borax	649:784	Here, multifunctional DN hydrogels are prepared via a combination of host-guest interaction of β-cyclodextrin and ferrocene with dynamic borate ester bonds of poly(vinyl alcohol) and borax.
33723988	9	12	theme	lung	1361:1364	arg1	movement					1343:1350	the respiratory movement	1327:1350	the respiratory movement of a pig lung	1327:1364	In addition, the hydrogel sensors may track the respiratory movement of a pig lung in vitro.
33723988	6	13	theme	fracture	953:960	arg1	strength					962:969	fracture strength	953:969	fracture strength (41.0 KPa)	953:980	The obtained DN composite hydrogels possess good biocompatibility, stretchability (436%), fracture strength (41.0 KPa), self-healing property (healing efficiency of 95%), and high tensile strain sensitivity (gauge factor of 5.9).
33723988	6	13	theme	fracture	953:960	arg1	KPa					977:979	41.0 KPa	972:979	41.0 KPa	972:979	The obtained DN composite hydrogels possess good biocompatibility, stretchability (436%), fracture strength (41.0 KPa), self-healing property (healing efficiency of 95%), and high tensile strain sensitivity (gauge factor of 5.9).
33723988	6	14	theme	of	1025:1026	arg1	property					996:1003	self-healing property	983:1003	self-healing property (healing efficiency of 95%)	983:1031	The obtained DN composite hydrogels possess good biocompatibility, stretchability (436%), fracture strength (41.0 KPa), self-healing property (healing efficiency of 95%), and high tensile strain sensitivity (gauge factor of 5.9).
33723988	6	14	theme	of	1025:1026	arg1	%					1030:1030	healing efficiency of 95%	1006:1030	healing efficiency of 95%	1006:1030	The obtained DN composite hydrogels possess good biocompatibility, stretchability (436%), fracture strength (41.0 KPa), self-healing property (healing efficiency of 95%), and high tensile strain sensitivity (gauge factor of 5.9).
33723988	4	15	theme	dynamic	726:732	arg1	bonds					747:751	dynamic borate ester bonds	726:751	dynamic borate ester bonds of poly(vinyl alcohol) and borax	726:784	Here, multifunctional DN hydrogels are prepared via a combination of host-guest interaction of β-cyclodextrin and ferrocene with dynamic borate ester bonds of poly(vinyl alcohol) and borax.
33723988	1	16	theme	soft	347:350	arg1	robotics					352:359	soft robotics	347:359	soft robotics	347:359	Hydrogel-based flexible strain sensors have shown great potential in body movement tracking, early disease diagnosis, noninvasive treatment, electronic skins, and soft robotics.
33723988	6	17	theme	healing	1006:1012	arg1	property					996:1003	self-healing property	983:1003	self-healing property (healing efficiency of 95%)	983:1031	The obtained DN composite hydrogels possess good biocompatibility, stretchability (436%), fracture strength (41.0 KPa), self-healing property (healing efficiency of 95%), and high tensile strain sensitivity (gauge factor of 5.9).
33723988	6	17	theme	healing	1006:1012	arg1	%					1030:1030	healing efficiency of 95%	1006:1030	healing efficiency of 95%	1006:1030	The obtained DN composite hydrogels possess good biocompatibility, stretchability (436%), fracture strength (41.0 KPa), self-healing property (healing efficiency of 95%), and high tensile strain sensitivity (gauge factor of 5.9).
33723988	4	18	theme	ester	741:745	arg1	bonds					747:751	dynamic borate ester bonds	726:751	dynamic borate ester bonds of poly(vinyl alcohol) and borax	726:784	Here, multifunctional DN hydrogels are prepared via a combination of host-guest interaction of β-cyclodextrin and ferrocene with dynamic borate ester bonds of poly(vinyl alcohol) and borax.
33723988	0	19	theme	Dynamic	95:101	arg1	Bond					112:115	Dynamic Covalent Bond	95:115	Dynamic Covalent Bond	95:115	Multifunctional Self-Healing Dual Network Hydrogels Constructed via Host-Guest Interaction and Dynamic Covalent Bond as Wearable Strain Sensors for Monitoring Human and Organ Motions.
33723988	7	20	theme	human	1176:1180	arg1	motions					1182:1188	different human motions	1166:1188	different human motions	1166:1188	The DN composite hydrogels are used as flexible strain sensors to detect different human motions.
33723988	7	21	theme	different	1166:1174	arg1	motions					1182:1188	different human motions	1166:1188	different human motions	1166:1188	The DN composite hydrogels are used as flexible strain sensors to detect different human motions.
33723988	2	22	theme	stretchable	414:424	arg1	properties					426:435	The good self-healing, biocompatible, sensitive and stretchable properties	362:435	The good self-healing, biocompatible, sensitive and stretchable properties	362:435	The good self-healing, biocompatible, sensitive and stretchable properties are the focus of hydrogel-based flexible strain sensors.
33723988	2	22	theme	stretchable	414:424	arg1	focus					445:449	the focus	441:449	the focus of hydrogel-based flexible strain sensors	441:491	The good self-healing, biocompatible, sensitive and stretchable properties are the focus of hydrogel-based flexible strain sensors.
33723988	0	23	theme	Wearable	120:127	arg1	Sensors					136:142	Wearable Strain Sensors	120:142	Wearable Strain Sensors for Monitoring Human and Organ Motions	120:181	Multifunctional Self-Healing Dual Network Hydrogels Constructed via Host-Guest Interaction and Dynamic Covalent Bond as Wearable Strain Sensors for Monitoring Human and Organ Motions.
33723988	6	24	theme	strain	1051:1056	arg1	sensitivity					1058:1068	high tensile strain sensitivity	1038:1068	high tensile strain sensitivity (gauge factor of 5.9)	1038:1090	The obtained DN composite hydrogels possess good biocompatibility, stretchability (436%), fracture strength (41.0 KPa), self-healing property (healing efficiency of 95%), and high tensile strain sensitivity (gauge factor of 5.9).
33723988	2	25	theme	strain	478:483	arg1	sensors					485:491	hydrogel-based flexible strain sensors	454:491	hydrogel-based flexible strain sensors	454:491	The good self-healing, biocompatible, sensitive and stretchable properties are the focus of hydrogel-based flexible strain sensors.
33723988	2	26	theme	sensitive	400:408	arg1	properties					426:435	The good self-healing, biocompatible, sensitive and stretchable properties	362:435	The good self-healing, biocompatible, sensitive and stretchable properties	362:435	The good self-healing, biocompatible, sensitive and stretchable properties are the focus of hydrogel-based flexible strain sensors.
33723988	2	26	theme	sensitive	400:408	arg1	focus					445:449	the focus	441:449	the focus of hydrogel-based flexible strain sensors	441:491	The good self-healing, biocompatible, sensitive and stretchable properties are the focus of hydrogel-based flexible strain sensors.
33723988	5	27	theme	DN	826:827	arg1	hydrogels					829:837	the DN hydrogels	822:837	the DN hydrogels with good conductivity	822:860	Carbon nanotubes are used to endow the DN hydrogels with good conductivity.
33723988	6	28	theme	high	1038:1041	arg1	sensitivity					1058:1068	high tensile strain sensitivity	1038:1068	high tensile strain sensitivity (gauge factor of 5.9)	1038:1090	The obtained DN composite hydrogels possess good biocompatibility, stretchability (436%), fracture strength (41.0 KPa), self-healing property (healing efficiency of 95%), and high tensile strain sensitivity (gauge factor of 5.9).
33723988	2	29	theme	hydrogel-based	454:467	arg1	sensors					485:491	hydrogel-based flexible strain sensors	454:491	hydrogel-based flexible strain sensors	454:491	The good self-healing, biocompatible, sensitive and stretchable properties are the focus of hydrogel-based flexible strain sensors.
33723988	10	30	theme	self-healing	1447:1458	arg1	hydrogels					1460:1468	multifunctional self-healing hydrogels	1431:1468	multifunctional self-healing hydrogels for constructing flexible strain sensors	1431:1509	This work exhibits new ideas and approaches to develop multifunctional self-healing hydrogels for constructing flexible strain sensors.
33723988	6	31	dep	sensitivity	1058:1068	arg1	factor					1077:1082	gauge factor	1071:1082	gauge factor of 5.9	1071:1089	The obtained DN composite hydrogels possess good biocompatibility, stretchability (436%), fracture strength (41.0 KPa), self-healing property (healing efficiency of 95%), and high tensile strain sensitivity (gauge factor of 5.9).
33723988	3	32	theme	Dual	494:497	arg1	network					499:505	Dual network	494:505	Dual network (DN) hydrogels	494:520	Dual network (DN) hydrogels are hopeful to fabricate self-healing hydrogels with the above properties.
33723988	0	33	theme	Dual	29:32	arg1	Hydrogels					42:50	Multifunctional Self-Healing Dual Network Hydrogels	0:50	Multifunctional Self-Healing Dual Network Hydrogels	0:50	Multifunctional Self-Healing Dual Network Hydrogels Constructed via Host-Guest Interaction and Dynamic Covalent Bond as Wearable Strain Sensors for Monitoring Human and Organ Motions.
33723988	7	34	used	used	1124:1127	arg2	sensors					1148:1154	flexible strain sensors	1132:1154	flexible strain sensors	1132:1154	The DN composite hydrogels are used as flexible strain sensors to detect different human motions.
33723988	7	34	used	used	1124:1127	arg2	hydrogels					1110:1118	The DN composite hydrogels	1093:1118	The DN composite hydrogels	1093:1118	The DN composite hydrogels are used as flexible strain sensors to detect different human motions.
33723988	7	35	theme	strain	1141:1146	arg1	hydrogels					1110:1118	The DN composite hydrogels	1093:1118	The DN composite hydrogels	1093:1118	The DN composite hydrogels are used as flexible strain sensors to detect different human motions.
33723988	7	35	theme	strain	1141:1146	arg1	sensors					1148:1154	flexible strain sensors	1132:1154	flexible strain sensors	1132:1154	The DN composite hydrogels are used as flexible strain sensors to detect different human motions.
33723988	1	36	theme	electronic	325:334	arg1	skins					336:340	electronic skins	325:340	electronic skins	325:340	Hydrogel-based flexible strain sensors have shown great potential in body movement tracking, early disease diagnosis, noninvasive treatment, electronic skins, and soft robotics.
33723988	6	37	theme	DN	876:877	arg1	hydrogels					889:897	The obtained DN composite hydrogels	863:897	The obtained DN composite hydrogels	863:897	The obtained DN composite hydrogels possess good biocompatibility, stretchability (436%), fracture strength (41.0 KPa), self-healing property (healing efficiency of 95%), and high tensile strain sensitivity (gauge factor of 5.9).
33723988	10	38	theme	flexible	1487:1494	arg1	sensors					1503:1509	flexible strain sensors	1487:1509	flexible strain sensors	1487:1509	This work exhibits new ideas and approaches to develop multifunctional self-healing hydrogels for constructing flexible strain sensors.
33723988	7	39	theme	composite	1100:1108	arg1	hydrogels					1110:1118	The DN composite hydrogels	1093:1118	The DN composite hydrogels	1093:1118	The DN composite hydrogels are used as flexible strain sensors to detect different human motions.
33723988	7	39	theme	composite	1100:1108	arg1	sensors					1148:1154	flexible strain sensors	1132:1154	flexible strain sensors	1132:1154	The DN composite hydrogels are used as flexible strain sensors to detect different human motions.
33723988	9	40	theme	respiratory	1331:1341	arg1	movement					1343:1350	the respiratory movement	1327:1350	the respiratory movement of a pig lung	1327:1364	In addition, the hydrogel sensors may track the respiratory movement of a pig lung in vitro.
33723988	6	41	theme	5.9	1087:1089	arg1	factor					1077:1082	gauge factor	1071:1082	gauge factor of 5.9	1071:1089	The obtained DN composite hydrogels possess good biocompatibility, stretchability (436%), fracture strength (41.0 KPa), self-healing property (healing efficiency of 95%), and high tensile strain sensitivity (gauge factor of 5.9).
33723988	2	42	theme	biocompatible	385:397	arg1	properties					426:435	The good self-healing, biocompatible, sensitive and stretchable properties	362:435	The good self-healing, biocompatible, sensitive and stretchable properties	362:435	The good self-healing, biocompatible, sensitive and stretchable properties are the focus of hydrogel-based flexible strain sensors.
33723988	2	42	theme	biocompatible	385:397	arg1	focus					445:449	the focus	441:449	the focus of hydrogel-based flexible strain sensors	441:491	The good self-healing, biocompatible, sensitive and stretchable properties are the focus of hydrogel-based flexible strain sensors.
33723988	1	43	theme	movement	258:265	arg1	tracking					267:274	body movement tracking	253:274	body movement tracking	253:274	Hydrogel-based flexible strain sensors have shown great potential in body movement tracking, early disease diagnosis, noninvasive treatment, electronic skins, and soft robotics.
33723988	0	44	theme	Host-Guest	68:77	arg1	Interaction					79:89	Host-Guest Interaction	68:89	Host-Guest Interaction	68:89	Multifunctional Self-Healing Dual Network Hydrogels Constructed via Host-Guest Interaction and Dynamic Covalent Bond as Wearable Strain Sensors for Monitoring Human and Organ Motions.
33723988	3	45	dep	network	499:505	arg1	DN					508:509	DN	508:509	DN	508:509	Dual network (DN) hydrogels are hopeful to fabricate self-healing hydrogels with the above properties.
33723988	5	46	theme	Carbon	787:792	arg1	nanotubes					794:802	Carbon nanotubes	787:802	Carbon nanotubes	787:802	Carbon nanotubes are used to endow the DN hydrogels with good conductivity.
33723988	2	47	theme	self-healing	371:382	arg1	properties					426:435	The good self-healing, biocompatible, sensitive and stretchable properties	362:435	The good self-healing, biocompatible, sensitive and stretchable properties	362:435	The good self-healing, biocompatible, sensitive and stretchable properties are the focus of hydrogel-based flexible strain sensors.
33723988	2	47	theme	self-healing	371:382	arg1	focus					445:449	the focus	441:449	the focus of hydrogel-based flexible strain sensors	441:491	The good self-healing, biocompatible, sensitive and stretchable properties are the focus of hydrogel-based flexible strain sensors.
33723988	9	48	theme	pig	1357:1359	arg1	lung					1361:1364	a pig lung	1355:1364	a pig lung	1355:1364	In addition, the hydrogel sensors may track the respiratory movement of a pig lung in vitro.
33723988	1	49	theme	disease	283:289	arg1	diagnosis					291:299	early disease diagnosis	277:299	early disease diagnosis	277:299	Hydrogel-based flexible strain sensors have shown great potential in body movement tracking, early disease diagnosis, noninvasive treatment, electronic skins, and soft robotics.
33723988	4	50	theme	DN	619:620	arg1	hydrogels					622:630	multifunctional DN hydrogels	603:630	multifunctional DN hydrogels	603:630	Here, multifunctional DN hydrogels are prepared via a combination of host-guest interaction of β-cyclodextrin and ferrocene with dynamic borate ester bonds of poly(vinyl alcohol) and borax.
33723988	3	51	theme	self-healing	547:558	arg1	hydrogels					560:568	self-healing hydrogels	547:568	self-healing hydrogels	547:568	Dual network (DN) hydrogels are hopeful to fabricate self-healing hydrogels with the above properties.
33723988	4	52	theme	ferrocene	711:719	arg1	interaction					677:687	host-guest interaction	666:687	host-guest interaction of β-cyclodextrin and ferrocene with dynamic borate ester bonds of poly(vinyl alcohol) and borax	666:784	Here, multifunctional DN hydrogels are prepared via a combination of host-guest interaction of β-cyclodextrin and ferrocene with dynamic borate ester bonds of poly(vinyl alcohol) and borax.
33723988	1	53	theme	Hydrogel-based	184:197	arg1	sensors					215:221	Hydrogel-based flexible strain sensors	184:221	Hydrogel-based flexible strain sensors	184:221	Hydrogel-based flexible strain sensors have shown great potential in body movement tracking, early disease diagnosis, noninvasive treatment, electronic skins, and soft robotics.
33723988	6	54	theme	good	907:910	arg1	biocompatibility					912:927	good biocompatibility	907:927	good biocompatibility	907:927	The obtained DN composite hydrogels possess good biocompatibility, stretchability (436%), fracture strength (41.0 KPa), self-healing property (healing efficiency of 95%), and high tensile strain sensitivity (gauge factor of 5.9).
33723988	1	55	theme	strain	208:213	arg1	sensors					215:221	Hydrogel-based flexible strain sensors	184:221	Hydrogel-based flexible strain sensors	184:221	Hydrogel-based flexible strain sensors have shown great potential in body movement tracking, early disease diagnosis, noninvasive treatment, electronic skins, and soft robotics.
33723988	3	56	theme	above	579:583	arg1	properties					585:594	the above properties	575:594	the above properties	575:594	Dual network (DN) hydrogels are hopeful to fabricate self-healing hydrogels with the above properties.
33723988	5	57	theme	good	844:847	arg1	conductivity					849:860	good conductivity	844:860	good conductivity	844:860	Carbon nanotubes are used to endow the DN hydrogels with good conductivity.
33723988	0	58	theme	Human	159:163	arg1	Motions					175:181	Human and Organ Motions	159:181	Motions	175:181	Multifunctional Self-Healing Dual Network Hydrogels Constructed via Host-Guest Interaction and Dynamic Covalent Bond as Wearable Strain Sensors for Monitoring Human and Organ Motions.
33723988	1	59	theme	great	234:238	arg1	potential					240:248	great potential	234:248	great potential	234:248	Hydrogel-based flexible strain sensors have shown great potential in body movement tracking, early disease diagnosis, noninvasive treatment, electronic skins, and soft robotics.
33723988	4	60	theme	host-guest	666:675	arg1	interaction					677:687	host-guest interaction	666:687	host-guest interaction of β-cyclodextrin and ferrocene with dynamic borate ester bonds of poly(vinyl alcohol) and borax	666:784	Here, multifunctional DN hydrogels are prepared via a combination of host-guest interaction of β-cyclodextrin and ferrocene with dynamic borate ester bonds of poly(vinyl alcohol) and borax.
33723988	0	61	theme	Organ	169:173	arg1	Motions					175:181	Human and Organ Motions	159:181	Motions	175:181	Multifunctional Self-Healing Dual Network Hydrogels Constructed via Host-Guest Interaction and Dynamic Covalent Bond as Wearable Strain Sensors for Monitoring Human and Organ Motions.
33723988	8	62	contain	have	1262:1265	arg2	stability					1272:1280	good stability	1267:1280	good stability	1267:1280	After cutting, the healed hydrogels also can monitor human motions and have good stability.
33723988	8	62	contain	have	1262:1265	arg1	hydrogels					1217:1225	the healed hydrogels	1206:1225	the healed hydrogels	1206:1225	After cutting, the healed hydrogels also can monitor human motions and have good stability.
33723988	8	63	theme	human	1244:1248	arg1	motions					1250:1256	human motions	1244:1256	human motions	1244:1256	After cutting, the healed hydrogels also can monitor human motions and have good stability.
33723988	4	64	theme	poly	756:759	arg1	bonds					747:751	dynamic borate ester bonds	726:751	dynamic borate ester bonds of poly(vinyl alcohol) and borax	726:784	Here, multifunctional DN hydrogels are prepared via a combination of host-guest interaction of β-cyclodextrin and ferrocene with dynamic borate ester bonds of poly(vinyl alcohol) and borax.
33723988	6	65	theme	efficiency	1014:1023	arg1	property					996:1003	self-healing property	983:1003	self-healing property (healing efficiency of 95%)	983:1031	The obtained DN composite hydrogels possess good biocompatibility, stretchability (436%), fracture strength (41.0 KPa), self-healing property (healing efficiency of 95%), and high tensile strain sensitivity (gauge factor of 5.9).
33723988	6	65	theme	efficiency	1014:1023	arg1	%					1030:1030	healing efficiency of 95%	1006:1030	healing efficiency of 95%	1006:1030	The obtained DN composite hydrogels possess good biocompatibility, stretchability (436%), fracture strength (41.0 KPa), self-healing property (healing efficiency of 95%), and high tensile strain sensitivity (gauge factor of 5.9).
33723988	8	66	theme	good	1267:1270	arg1	stability					1272:1280	good stability	1267:1280	good stability	1267:1280	After cutting, the healed hydrogels also can monitor human motions and have good stability.
33723988	0	67	theme	Covalent	103:110	arg1	Bond					112:115	Dynamic Covalent Bond	95:115	Dynamic Covalent Bond	95:115	Multifunctional Self-Healing Dual Network Hydrogels Constructed via Host-Guest Interaction and Dynamic Covalent Bond as Wearable Strain Sensors for Monitoring Human and Organ Motions.
33723988	4	68	theme	borate	734:739	arg1	bonds					747:751	dynamic borate ester bonds	726:751	dynamic borate ester bonds of poly(vinyl alcohol) and borax	726:784	Here, multifunctional DN hydrogels are prepared via a combination of host-guest interaction of β-cyclodextrin and ferrocene with dynamic borate ester bonds of poly(vinyl alcohol) and borax.
33723988	6	69	theme	self-healing	983:994	arg1	property					996:1003	self-healing property	983:1003	self-healing property (healing efficiency of 95%)	983:1031	The obtained DN composite hydrogels possess good biocompatibility, stretchability (436%), fracture strength (41.0 KPa), self-healing property (healing efficiency of 95%), and high tensile strain sensitivity (gauge factor of 5.9).
33723988	6	69	theme	self-healing	983:994	arg1	%					1030:1030	healing efficiency of 95%	1006:1030	healing efficiency of 95%	1006:1030	The obtained DN composite hydrogels possess good biocompatibility, stretchability (436%), fracture strength (41.0 KPa), self-healing property (healing efficiency of 95%), and high tensile strain sensitivity (gauge factor of 5.9).
33723988	4	70	theme	borax	780:784	arg1	bonds					747:751	dynamic borate ester bonds	726:751	dynamic borate ester bonds of poly(vinyl alcohol) and borax	726:784	Here, multifunctional DN hydrogels are prepared via a combination of host-guest interaction of β-cyclodextrin and ferrocene with dynamic borate ester bonds of poly(vinyl alcohol) and borax.
33723988	0	71	theme	Strain	129:134	arg1	Sensors					136:142	Wearable Strain Sensors	120:142	Wearable Strain Sensors for Monitoring Human and Organ Motions	120:181	Multifunctional Self-Healing Dual Network Hydrogels Constructed via Host-Guest Interaction and Dynamic Covalent Bond as Wearable Strain Sensors for Monitoring Human and Organ Motions.
33723988	0	72	theme	Self-Healing	16:27	arg1	Hydrogels					42:50	Multifunctional Self-Healing Dual Network Hydrogels	0:50	Multifunctional Self-Healing Dual Network Hydrogels	0:50	Multifunctional Self-Healing Dual Network Hydrogels Constructed via Host-Guest Interaction and Dynamic Covalent Bond as Wearable Strain Sensors for Monitoring Human and Organ Motions.
33723988	6	73	theme	tensile	1043:1049	arg1	sensitivity					1058:1068	high tensile strain sensitivity	1038:1068	high tensile strain sensitivity (gauge factor of 5.9)	1038:1090	The obtained DN composite hydrogels possess good biocompatibility, stretchability (436%), fracture strength (41.0 KPa), self-healing property (healing efficiency of 95%), and high tensile strain sensitivity (gauge factor of 5.9).
33723988	2	74	theme	flexible	469:476	arg1	sensors					485:491	hydrogel-based flexible strain sensors	454:491	hydrogel-based flexible strain sensors	454:491	The good self-healing, biocompatible, sensitive and stretchable properties are the focus of hydrogel-based flexible strain sensors.
33723988	10	75	theme	new	1395:1397	arg1	ideas					1399:1403	new ideas	1395:1403	new ideas	1395:1403	This work exhibits new ideas and approaches to develop multifunctional self-healing hydrogels for constructing flexible strain sensors.
33723988	4	76	theme	vinyl	761:765	arg1	poly					756:759	poly	756:759	poly(vinyl alcohol)	756:774	Here, multifunctional DN hydrogels are prepared via a combination of host-guest interaction of β-cyclodextrin and ferrocene with dynamic borate ester bonds of poly(vinyl alcohol) and borax.
33723988	4	76	theme	vinyl	761:765	arg1	alcohol					767:773	vinyl alcohol	761:773	vinyl alcohol	761:773	Here, multifunctional DN hydrogels are prepared via a combination of host-guest interaction of β-cyclodextrin and ferrocene with dynamic borate ester bonds of poly(vinyl alcohol) and borax.
33723988	0	77	theme	Network	34:40	arg1	Hydrogels					42:50	Multifunctional Self-Healing Dual Network Hydrogels	0:50	Multifunctional Self-Healing Dual Network Hydrogels	0:50	Multifunctional Self-Healing Dual Network Hydrogels Constructed via Host-Guest Interaction and Dynamic Covalent Bond as Wearable Strain Sensors for Monitoring Human and Organ Motions.
33723988	1	78	theme	noninvasive	302:312	arg1	treatment					314:322	noninvasive treatment	302:322	noninvasive treatment	302:322	Hydrogel-based flexible strain sensors have shown great potential in body movement tracking, early disease diagnosis, noninvasive treatment, electronic skins, and soft robotics.
33723988	10	79	theme	multifunctional	1431:1445	arg1	hydrogels					1460:1468	multifunctional self-healing hydrogels	1431:1468	multifunctional self-healing hydrogels for constructing flexible strain sensors	1431:1509	This work exhibits new ideas and approaches to develop multifunctional self-healing hydrogels for constructing flexible strain sensors.
33723988	3	80	theme	network	499:505	arg1	hydrogels					512:520	Dual network (DN) hydrogels	494:520	Dual network (DN) hydrogels	494:520	Dual network (DN) hydrogels are hopeful to fabricate self-healing hydrogels with the above properties.
33723988	9	81	theme	hydrogel	1300:1307	arg1	sensors					1309:1315	the hydrogel sensors	1296:1315	the hydrogel sensors	1296:1315	In addition, the hydrogel sensors may track the respiratory movement of a pig lung in vitro.
33723988	5	82	used	used	808:811	arg2	nanotubes					794:802	Carbon nanotubes	787:802	Carbon nanotubes	787:802	Carbon nanotubes are used to endow the DN hydrogels with good conductivity.
33723988	6	83	theme	composite	879:887	arg1	hydrogels					889:897	The obtained DN composite hydrogels	863:897	The obtained DN composite hydrogels	863:897	The obtained DN composite hydrogels possess good biocompatibility, stretchability (436%), fracture strength (41.0 KPa), self-healing property (healing efficiency of 95%), and high tensile strain sensitivity (gauge factor of 5.9).
33723988	7	84	theme	DN	1097:1098	arg1	hydrogels					1110:1118	The DN composite hydrogels	1093:1118	The DN composite hydrogels	1093:1118	The DN composite hydrogels are used as flexible strain sensors to detect different human motions.
33723988	7	84	theme	DN	1097:1098	arg1	sensors					1148:1154	flexible strain sensors	1132:1154	flexible strain sensors	1132:1154	The DN composite hydrogels are used as flexible strain sensors to detect different human motions.
33723988	6	85	theme	obtained	867:874	arg1	hydrogels					889:897	The obtained DN composite hydrogels	863:897	The obtained DN composite hydrogels	863:897	The obtained DN composite hydrogels possess good biocompatibility, stretchability (436%), fracture strength (41.0 KPa), self-healing property (healing efficiency of 95%), and high tensile strain sensitivity (gauge factor of 5.9).
33723988	2	86	theme	sensors	485:491	arg1	properties					426:435	The good self-healing, biocompatible, sensitive and stretchable properties	362:435	The good self-healing, biocompatible, sensitive and stretchable properties	362:435	The good self-healing, biocompatible, sensitive and stretchable properties are the focus of hydrogel-based flexible strain sensors.
33723988	2	86	theme	sensors	485:491	arg1	focus					445:449	the focus	441:449	the focus of hydrogel-based flexible strain sensors	441:491	The good self-healing, biocompatible, sensitive and stretchable properties are the focus of hydrogel-based flexible strain sensors.
33723988	4	87	with	interaction	677:687	arg1	bonds					747:751	dynamic borate ester bonds	726:751	dynamic borate ester bonds of poly(vinyl alcohol) and borax	726:784	Here, multifunctional DN hydrogels are prepared via a combination of host-guest interaction of β-cyclodextrin and ferrocene with dynamic borate ester bonds of poly(vinyl alcohol) and borax.
33723988	1	88	theme	body	253:256	arg1	tracking					267:274	body movement tracking	253:274	body movement tracking	253:274	Hydrogel-based flexible strain sensors have shown great potential in body movement tracking, early disease diagnosis, noninvasive treatment, electronic skins, and soft robotics.
33723988	7	89	theme	flexible	1132:1139	arg1	hydrogels					1110:1118	The DN composite hydrogels	1093:1118	The DN composite hydrogels	1093:1118	The DN composite hydrogels are used as flexible strain sensors to detect different human motions.
33723988	7	89	theme	flexible	1132:1139	arg1	sensors					1148:1154	flexible strain sensors	1132:1154	flexible strain sensors	1132:1154	The DN composite hydrogels are used as flexible strain sensors to detect different human motions.
32886302	7	0	theme	structured	1272:1281	arg1	particles					1291:1299	hexagonally structured primary particles	1260:1299	hexagonally structured primary particles	1260:1299	RESULTS On TEM, all samples showed similar shape with hexagonally structured primary particles, agglomerated in clusters.
32886302	4	1	theme	gallium	607:613	arg1	nanoparticles					622:634	stable gallium carbon nanoparticles	600:634	stable gallium carbon nanoparticles	600:634	PROCEDURES 99mTc- and 68Ga-labelled carbon nanoparticles, stable gallium carbon nanoparticles, 0.9 % NaCl and 0.1 N HCl-based carbon nanoparticles were produced using an unmodified Technegas® generator, following the usual technique used for clinical Technegas® production.
32886302	3	2	theme	carbon	512:517	arg1	aerosol					533:539	99mTc-labelled carbon nanoparticles aerosol	497:539	99mTc-labelled carbon nanoparticles aerosol	497:539	The aim of this study therefore was to evaluate the shape and size of 68Ga-labelled carbon nanoparticles, and to determine the composition of the aerosol, as opposed to 99mTc-labelled carbon nanoparticles aerosol.
32886302	2	3	theme	68Ga-labelled	292:304	arg1	nanoparticles					313:325	68Ga-labelled carbon nanoparticles	292:325	68Ga-labelled carbon nanoparticles	292:325	However, no study has assessed the physical properties of 68Ga-labelled carbon nanoparticles.
32886302	5	4	dep	shape	820:824	arg1	The					816:818	The	816:818	The	816:818	The shape and size of particles were studied by transmission electron microscopy (TEM) after decay of the radioactive samples.
32886302	1	5	theme	lung	201:204	arg1	imaging					225:231	lung ventilation PET/CT imaging	201:231	lung ventilation PET/CT imaging	201:231	PURPOSE The use of 68Ga-labelled carbon nanoparticles has been proposed for lung ventilation PET/CT imaging.
32886302	6	6	theme	nanoparticles	1177:1189	arg1	decay					1132:1136	decay	1132:1136	decay of the 68Ga- and 99mTc-labelled carbon nanoparticles	1132:1189	The composition of the 68Zn- and 99Tc-labelled carbon nanoparticles aerosols was assessed using scanning electron microscopy (SEM) coupled with energy dispersive X-ray (EDX) analysis after decay of the 68Ga- and 99mTc-labelled carbon nanoparticles, respectively.
32886302	2	7	theme	nanoparticles	313:325	arg1	properties					278:287	the physical properties	265:287	the physical properties of 68Ga-labelled carbon nanoparticles	265:325	However, no study has assessed the physical properties of 68Ga-labelled carbon nanoparticles.
32886302	5	8	theme	electron	877:884	arg1	microscopy					886:895	transmission electron microscopy	864:895	transmission electron microscopy (TEM)	864:901	The shape and size of particles were studied by transmission electron microscopy (TEM) after decay of the radioactive samples.
32886302	5	8	theme	electron	877:884	arg1	TEM					898:900	TEM	898:900	TEM	898:900	The shape and size of particles were studied by transmission electron microscopy (TEM) after decay of the radioactive samples.
32886302	6	9	theme	carbon	990:995	arg1	nanoparticles					997:1009	99Tc-labelled carbon nanoparticles	976:1009	99Tc-labelled carbon nanoparticles	976:1009	The composition of the 68Zn- and 99Tc-labelled carbon nanoparticles aerosols was assessed using scanning electron microscopy (SEM) coupled with energy dispersive X-ray (EDX) analysis after decay of the 68Ga- and 99mTc-labelled carbon nanoparticles, respectively.
32886302	4	10	theme	carbon	668:673	arg1	nanoparticles					675:687	0.1 N HCl-based carbon nanoparticles	652:687	0.1 N HCl-based carbon nanoparticles	652:687	PROCEDURES 99mTc- and 68Ga-labelled carbon nanoparticles, stable gallium carbon nanoparticles, 0.9 % NaCl and 0.1 N HCl-based carbon nanoparticles were produced using an unmodified Technegas® generator, following the usual technique used for clinical Technegas® production.
32886302	9	11	theme	clinical	1558:1565	arg1	way					1567:1569	the usual clinical way	1548:1569	the usual clinical way	1548:1569	CONCLUSION Using Technegas® generator in the usual clinical way, 99mTc- and 68Ga-labelled carbon nanoparticles demonstrated similar shape and diameters in the same size range size.
32886302	8	12	theme	mean	1332:1335	arg1	diameters					1337:1345	The mean diameters	1328:1345	The mean diameters of primary stable gallium carbon nanoparticles, 99Tc- and 68Zn-labelled carbon nanoparticles	1328:1438	The mean diameters of primary stable gallium carbon nanoparticles, 99Tc- and 68Zn-labelled carbon nanoparticles were 22.4 ± 10 nm, 20.9 ± 7.2 nm and 19.8 ± 11.7 nm, respectively.
32886302	8	12	theme	mean	1332:1335	arg1	22.4 ± 10 nm					1445:1456	22.4 ± 10 nm	1445:1456	22.4 ± 10 nm	1445:1456	The mean diameters of primary stable gallium carbon nanoparticles, 99Tc- and 68Zn-labelled carbon nanoparticles were 22.4 ± 10 nm, 20.9 ± 7.2 nm and 19.8 ± 11.7 nm, respectively.
32886302	4	13	theme	usual	759:763	arg1	technique					765:773	the usual technique	755:773	the usual technique used for clinical Technegas® production	755:813	PROCEDURES 99mTc- and 68Ga-labelled carbon nanoparticles, stable gallium carbon nanoparticles, 0.9 % NaCl and 0.1 N HCl-based carbon nanoparticles were produced using an unmodified Technegas® generator, following the usual technique used for clinical Technegas® production.
32886302	4	14	theme	clinical	784:791	arg1	production					804:813	clinical Technegas® production	784:813	clinical Technegas® production	784:813	PROCEDURES 99mTc- and 68Ga-labelled carbon nanoparticles, stable gallium carbon nanoparticles, 0.9 % NaCl and 0.1 N HCl-based carbon nanoparticles were produced using an unmodified Technegas® generator, following the usual technique used for clinical Technegas® production.
32886302	3	15	theme	study	344:348	arg1	aim					332:334	The aim	328:334	The aim of this study	328:348	The aim of this study therefore was to evaluate the shape and size of 68Ga-labelled carbon nanoparticles, and to determine the composition of the aerosol, as opposed to 99mTc-labelled carbon nanoparticles aerosol.
32886302	4	16	theme	unmodified	712:721	arg1	generator					734:742	an unmodified Technegas® generator	709:742	an unmodified Technegas® generator	709:742	PROCEDURES 99mTc- and 68Ga-labelled carbon nanoparticles, stable gallium carbon nanoparticles, 0.9 % NaCl and 0.1 N HCl-based carbon nanoparticles were produced using an unmodified Technegas® generator, following the usual technique used for clinical Technegas® production.
32886302	0	17	theme	Physical	67:74	arg1	Study					87:91	Study	87:91	Study	87:91	68Ga-Labelled Carbon Nanoparticles for Ventilation PET/CT Imaging: Physical Properties Study and Comparison with Technegas®.
32886302	0	17	theme	Physical	67:74	arg1	Properties					76:85	Imaging: Physical Properties	58:85	Imaging: Physical Properties Study and Comparison with Technegas®	58:122	68Ga-Labelled Carbon Nanoparticles for Ventilation PET/CT Imaging: Physical Properties Study and Comparison with Technegas®.
32886302	0	17	theme	Physical	67:74	arg1	Comparison					97:106	Comparison	97:106	Comparison with Technegas®	97:122	68Ga-Labelled Carbon Nanoparticles for Ventilation PET/CT Imaging: Physical Properties Study and Comparison with Technegas®.
32886302	9	18	theme	carbon	1597:1602	arg1	nanoparticles					1604:1616	99mTc- and 68Ga-labelled carbon nanoparticles	1572:1616	99mTc- and 68Ga-labelled carbon nanoparticles	1572:1616	CONCLUSION Using Technegas® generator in the usual clinical way, 99mTc- and 68Ga-labelled carbon nanoparticles demonstrated similar shape and diameters in the same size range size.
32886302	3	19	dep	shape	380:384	arg1	the					376:378	the	376:378	the	376:378	The aim of this study therefore was to evaluate the shape and size of 68Ga-labelled carbon nanoparticles, and to determine the composition of the aerosol, as opposed to 99mTc-labelled carbon nanoparticles aerosol.
32886302	8	20	theme	carbon	1373:1378	arg1	nanoparticles					1380:1392	primary stable gallium carbon nanoparticles	1350:1392	primary stable gallium carbon nanoparticles	1350:1392	The mean diameters of primary stable gallium carbon nanoparticles, 99Tc- and 68Zn-labelled carbon nanoparticles were 22.4 ± 10 nm, 20.9 ± 7.2 nm and 19.8 ± 11.7 nm, respectively.
32886302	4	21	dep	PROCEDURES	542:551	arg1	99mTc-					553:558	99mTc-	553:558	99mTc-	553:558	PROCEDURES 99mTc- and 68Ga-labelled carbon nanoparticles, stable gallium carbon nanoparticles, 0.9 % NaCl and 0.1 N HCl-based carbon nanoparticles were produced using an unmodified Technegas® generator, following the usual technique used for clinical Technegas® production.
32886302	4	21	dep	PROCEDURES	542:551	arg1	nanoparticles					585:597	68Ga-labelled carbon nanoparticles	564:597	68Ga-labelled carbon nanoparticles	564:597	PROCEDURES 99mTc- and 68Ga-labelled carbon nanoparticles, stable gallium carbon nanoparticles, 0.9 % NaCl and 0.1 N HCl-based carbon nanoparticles were produced using an unmodified Technegas® generator, following the usual technique used for clinical Technegas® production.
32886302	4	21	dep	PROCEDURES	542:551	arg1	PROCEDURES					542:551	PROCEDURES 99mTc- and 68Ga-labelled carbon nanoparticles, stable gallium carbon nanoparticles, 0.9 % NaCl and 0.1 N HCl-based carbon nanoparticles	542:687	PROCEDURES 99mTc- and 68Ga-labelled carbon nanoparticles, stable gallium carbon nanoparticles, 0.9 % NaCl and 0.1 N HCl-based carbon nanoparticles	542:687	PROCEDURES 99mTc- and 68Ga-labelled carbon nanoparticles, stable gallium carbon nanoparticles, 0.9 % NaCl and 0.1 N HCl-based carbon nanoparticles were produced using an unmodified Technegas® generator, following the usual technique used for clinical Technegas® production.
32886302	5	22	theme	samples	934:940	arg1	decay					909:913	decay	909:913	decay of the radioactive samples	909:940	The shape and size of particles were studied by transmission electron microscopy (TEM) after decay of the radioactive samples.
32886302	6	23	theme	scanning	1039:1046	arg1	SEM					1069:1071	SEM	1069:1071	SEM	1069:1071	The composition of the 68Zn- and 99Tc-labelled carbon nanoparticles aerosols was assessed using scanning electron microscopy (SEM) coupled with energy dispersive X-ray (EDX) analysis after decay of the 68Ga- and 99mTc-labelled carbon nanoparticles, respectively.
32886302	6	23	theme	scanning	1039:1046	arg1	microscopy					1057:1066	scanning electron microscopy	1039:1066	scanning electron microscopy (SEM) coupled with energy dispersive X-ray (EDX) analysis after decay of the 68Ga- and 99mTc-labelled carbon nanoparticles	1039:1189	The composition of the 68Zn- and 99Tc-labelled carbon nanoparticles aerosols was assessed using scanning electron microscopy (SEM) coupled with energy dispersive X-ray (EDX) analysis after decay of the 68Ga- and 99mTc-labelled carbon nanoparticles, respectively.
32886302	1	24	dep	PURPOSE	125:131	arg1	proposed					188:195	proposed	188:195	has been proposed for lung ventilation PET/CT imaging	179:231	PURPOSE The use of 68Ga-labelled carbon nanoparticles has been proposed for lung ventilation PET/CT imaging.
32886302	10	25	theme	nanoparticles	1742:1754	arg1	use					1714:1716	the use	1710:1716	the use of 68Ga-labelled carbon nanoparticles for the assessment of regional lung ventilation	1710:1802	These results support the use of 68Ga-labelled carbon nanoparticles for the assessment of regional lung ventilation function with PET imaging.
32886302	6	26	theme	EDX	1112:1114	arg1	analysis					1117:1124	energy dispersive X-ray (EDX) analysis	1087:1124	energy dispersive X-ray (EDX) analysis after decay of the 68Ga- and 99mTc-labelled carbon nanoparticles	1087:1189	The composition of the 68Zn- and 99Tc-labelled carbon nanoparticles aerosols was assessed using scanning electron microscopy (SEM) coupled with energy dispersive X-ray (EDX) analysis after decay of the 68Ga- and 99mTc-labelled carbon nanoparticles, respectively.
32886302	8	27	theme	carbon	1419:1424	arg1	nanoparticles					1426:1438	68Zn-labelled carbon nanoparticles	1405:1438	68Zn-labelled carbon nanoparticles	1405:1438	The mean diameters of primary stable gallium carbon nanoparticles, 99Tc- and 68Zn-labelled carbon nanoparticles were 22.4 ± 10 nm, 20.9 ± 7.2 nm and 19.8 ± 11.7 nm, respectively.
32886302	10	28	theme	68Ga-labelled	1721:1733	arg1	nanoparticles					1742:1754	68Ga-labelled carbon nanoparticles	1721:1754	68Ga-labelled carbon nanoparticles	1721:1754	These results support the use of 68Ga-labelled carbon nanoparticles for the assessment of regional lung ventilation function with PET imaging.
32886302	0	29	theme	68Ga-Labelled	0:12	arg1	Nanoparticles					21:33	68Ga-Labelled Carbon Nanoparticles	0:33	68Ga-Labelled Carbon Nanoparticles for Ventilation	0:49	68Ga-Labelled Carbon Nanoparticles for Ventilation PET/CT Imaging: Physical Properties Study and Comparison with Technegas®.
32886302	6	30	theme	X-ray	1105:1109	arg1	analysis					1117:1124	energy dispersive X-ray (EDX) analysis	1087:1124	energy dispersive X-ray (EDX) analysis after decay of the 68Ga- and 99mTc-labelled carbon nanoparticles	1087:1189	The composition of the 68Zn- and 99Tc-labelled carbon nanoparticles aerosols was assessed using scanning electron microscopy (SEM) coupled with energy dispersive X-ray (EDX) analysis after decay of the 68Ga- and 99mTc-labelled carbon nanoparticles, respectively.
32886302	4	31	theme	68Ga-labelled	564:576	arg1	nanoparticles					585:597	68Ga-labelled carbon nanoparticles	564:597	68Ga-labelled carbon nanoparticles	564:597	PROCEDURES 99mTc- and 68Ga-labelled carbon nanoparticles, stable gallium carbon nanoparticles, 0.9 % NaCl and 0.1 N HCl-based carbon nanoparticles were produced using an unmodified Technegas® generator, following the usual technique used for clinical Technegas® production.
32886302	8	32	theme	gallium	1365:1371	arg1	nanoparticles					1380:1392	primary stable gallium carbon nanoparticles	1350:1392	primary stable gallium carbon nanoparticles	1350:1392	The mean diameters of primary stable gallium carbon nanoparticles, 99Tc- and 68Zn-labelled carbon nanoparticles were 22.4 ± 10 nm, 20.9 ± 7.2 nm and 19.8 ± 11.7 nm, respectively.
32886302	9	33	theme	size	1671:1674	arg1	range					1676:1680	the same size range	1662:1680	the same size range	1662:1680	CONCLUSION Using Technegas® generator in the usual clinical way, 99mTc- and 68Ga-labelled carbon nanoparticles demonstrated similar shape and diameters in the same size range size.
32886302	6	34	theme	energy	1087:1092	arg1	analysis					1117:1124	energy dispersive X-ray (EDX) analysis	1087:1124	energy dispersive X-ray (EDX) analysis after decay of the 68Ga- and 99mTc-labelled carbon nanoparticles	1087:1189	The composition of the 68Zn- and 99Tc-labelled carbon nanoparticles aerosols was assessed using scanning electron microscopy (SEM) coupled with energy dispersive X-ray (EDX) analysis after decay of the 68Ga- and 99mTc-labelled carbon nanoparticles, respectively.
32886302	3	35	theme	nanoparticles	419:431	arg1	size					390:393	size	390:393	size	390:393	The aim of this study therefore was to evaluate the shape and size of 68Ga-labelled carbon nanoparticles, and to determine the composition of the aerosol, as opposed to 99mTc-labelled carbon nanoparticles aerosol.
32886302	3	35	theme	nanoparticles	419:431	arg1	shape					380:384	shape	380:384	shape	380:384	The aim of this study therefore was to evaluate the shape and size of 68Ga-labelled carbon nanoparticles, and to determine the composition of the aerosol, as opposed to 99mTc-labelled carbon nanoparticles aerosol.
32886302	3	36	theme	68Ga-labelled	398:410	arg1	nanoparticles					419:431	68Ga-labelled carbon nanoparticles	398:431	68Ga-labelled carbon nanoparticles	398:431	The aim of this study therefore was to evaluate the shape and size of 68Ga-labelled carbon nanoparticles, and to determine the composition of the aerosol, as opposed to 99mTc-labelled carbon nanoparticles aerosol.
32886302	0	37	theme	Imaging	58:64	arg1	Study					87:91	Study	87:91	Study	87:91	68Ga-Labelled Carbon Nanoparticles for Ventilation PET/CT Imaging: Physical Properties Study and Comparison with Technegas®.
32886302	0	37	theme	Imaging	58:64	arg1	Properties					76:85	Imaging: Physical Properties	58:85	Imaging: Physical Properties Study and Comparison with Technegas®	58:122	68Ga-Labelled Carbon Nanoparticles for Ventilation PET/CT Imaging: Physical Properties Study and Comparison with Technegas®.
32886302	0	37	theme	Imaging	58:64	arg1	Comparison					97:106	Comparison	97:106	Comparison with Technegas®	97:122	68Ga-Labelled Carbon Nanoparticles for Ventilation PET/CT Imaging: Physical Properties Study and Comparison with Technegas®.
32886302	10	38	theme	lung	1787:1790	arg1	ventilation					1792:1802	regional lung ventilation	1778:1802	regional lung ventilation	1778:1802	These results support the use of 68Ga-labelled carbon nanoparticles for the assessment of regional lung ventilation function with PET imaging.
32886302	8	39	theme	stable	1358:1363	arg1	nanoparticles					1380:1392	primary stable gallium carbon nanoparticles	1350:1392	primary stable gallium carbon nanoparticles	1350:1392	The mean diameters of primary stable gallium carbon nanoparticles, 99Tc- and 68Zn-labelled carbon nanoparticles were 22.4 ± 10 nm, 20.9 ± 7.2 nm and 19.8 ± 11.7 nm, respectively.
32886302	1	40	theme	carbon	158:163	arg1	nanoparticles					165:177	68Ga-labelled carbon nanoparticles	144:177	68Ga-labelled carbon nanoparticles	144:177	PURPOSE The use of 68Ga-labelled carbon nanoparticles has been proposed for lung ventilation PET/CT imaging.
32886302	3	41	theme	nanoparticles	519:531	arg1	aerosol					533:539	99mTc-labelled carbon nanoparticles aerosol	497:539	99mTc-labelled carbon nanoparticles aerosol	497:539	The aim of this study therefore was to evaluate the shape and size of 68Ga-labelled carbon nanoparticles, and to determine the composition of the aerosol, as opposed to 99mTc-labelled carbon nanoparticles aerosol.
32886302	4	42	theme	%	641:641	arg1	PROCEDURES					542:551	PROCEDURES 99mTc- and 68Ga-labelled carbon nanoparticles, stable gallium carbon nanoparticles, 0.9 % NaCl and 0.1 N HCl-based carbon nanoparticles	542:687	PROCEDURES 99mTc- and 68Ga-labelled carbon nanoparticles, stable gallium carbon nanoparticles, 0.9 % NaCl and 0.1 N HCl-based carbon nanoparticles	542:687	PROCEDURES 99mTc- and 68Ga-labelled carbon nanoparticles, stable gallium carbon nanoparticles, 0.9 % NaCl and 0.1 N HCl-based carbon nanoparticles were produced using an unmodified Technegas® generator, following the usual technique used for clinical Technegas® production.
32886302	4	42	theme	%	641:641	arg1	NaCl					643:646	0.9 % NaCl	637:646	0.9 % NaCl	637:646	PROCEDURES 99mTc- and 68Ga-labelled carbon nanoparticles, stable gallium carbon nanoparticles, 0.9 % NaCl and 0.1 N HCl-based carbon nanoparticles were produced using an unmodified Technegas® generator, following the usual technique used for clinical Technegas® production.
32886302	10	43	dep	support	1702:1708	arg1	function					1804:1811	function	1804:1811	support the use of 68Ga-labelled carbon nanoparticles for the assessment of regional lung ventilation function with PET imaging	1702:1828	These results support the use of 68Ga-labelled carbon nanoparticles for the assessment of regional lung ventilation function with PET imaging.
32886302	3	44	theme	99mTc-labelled	497:510	arg1	aerosol					533:539	99mTc-labelled carbon nanoparticles aerosol	497:539	99mTc-labelled carbon nanoparticles aerosol	497:539	The aim of this study therefore was to evaluate the shape and size of 68Ga-labelled carbon nanoparticles, and to determine the composition of the aerosol, as opposed to 99mTc-labelled carbon nanoparticles aerosol.
32886302	7	45	theme	primary	1283:1289	arg1	particles					1291:1299	hexagonally structured primary particles	1260:1299	hexagonally structured primary particles	1260:1299	RESULTS On TEM, all samples showed similar shape with hexagonally structured primary particles, agglomerated in clusters.
32886302	4	46	theme	stable	600:605	arg1	nanoparticles					622:634	stable gallium carbon nanoparticles	600:634	stable gallium carbon nanoparticles	600:634	PROCEDURES 99mTc- and 68Ga-labelled carbon nanoparticles, stable gallium carbon nanoparticles, 0.9 % NaCl and 0.1 N HCl-based carbon nanoparticles were produced using an unmodified Technegas® generator, following the usual technique used for clinical Technegas® production.
32886302	0	47	with	Study	87:91	arg1	Technegas®					113:122	Technegas®	113:122	Technegas®	113:122	68Ga-Labelled Carbon Nanoparticles for Ventilation PET/CT Imaging: Physical Properties Study and Comparison with Technegas®.
32886302	2	48	theme	carbon	306:311	arg1	nanoparticles					313:325	68Ga-labelled carbon nanoparticles	292:325	68Ga-labelled carbon nanoparticles	292:325	However, no study has assessed the physical properties of 68Ga-labelled carbon nanoparticles.
32886302	5	49	theme	radioactive	922:932	arg1	samples					934:940	the radioactive samples	918:940	the radioactive samples	918:940	The shape and size of particles were studied by transmission electron microscopy (TEM) after decay of the radioactive samples.
32886302	4	50	theme	carbon	615:620	arg1	nanoparticles					622:634	stable gallium carbon nanoparticles	600:634	stable gallium carbon nanoparticles	600:634	PROCEDURES 99mTc- and 68Ga-labelled carbon nanoparticles, stable gallium carbon nanoparticles, 0.9 % NaCl and 0.1 N HCl-based carbon nanoparticles were produced using an unmodified Technegas® generator, following the usual technique used for clinical Technegas® production.
32886302	9	51	theme	Technegas®	1524:1533	arg1	generator					1535:1543	Technegas® generator	1524:1543	Technegas® generator	1524:1543	CONCLUSION Using Technegas® generator in the usual clinical way, 99mTc- and 68Ga-labelled carbon nanoparticles demonstrated similar shape and diameters in the same size range size.
32886302	0	52	with	Comparison	97:106	arg1	Technegas®					113:122	Technegas®	113:122	Technegas®	113:122	68Ga-Labelled Carbon Nanoparticles for Ventilation PET/CT Imaging: Physical Properties Study and Comparison with Technegas®.
32886302	1	53	theme	ventilation	206:216	arg1	imaging					225:231	lung ventilation PET/CT imaging	201:231	lung ventilation PET/CT imaging	201:231	PURPOSE The use of 68Ga-labelled carbon nanoparticles has been proposed for lung ventilation PET/CT imaging.
32886302	6	54	theme	nanoparticles	997:1009	arg1	composition					947:957	The composition	943:957	The composition of the 68Zn- and 99Tc-labelled carbon nanoparticles aerosols	943:1018	The composition of the 68Zn- and 99Tc-labelled carbon nanoparticles aerosols was assessed using scanning electron microscopy (SEM) coupled with energy dispersive X-ray (EDX) analysis after decay of the 68Ga- and 99mTc-labelled carbon nanoparticles, respectively.
32886302	6	55	dep	68Zn-	966:970	arg1	aerosols					1011:1018	aerosols	1011:1018	aerosols	1011:1018	The composition of the 68Zn- and 99Tc-labelled carbon nanoparticles aerosols was assessed using scanning electron microscopy (SEM) coupled with energy dispersive X-ray (EDX) analysis after decay of the 68Ga- and 99mTc-labelled carbon nanoparticles, respectively.
32886302	6	56	theme	carbon	1170:1175	arg1	nanoparticles					1177:1189	the 68Ga- and 99mTc-labelled carbon nanoparticles	1141:1189	the 68Ga- and 99mTc-labelled carbon nanoparticles	1141:1189	The composition of the 68Zn- and 99Tc-labelled carbon nanoparticles aerosols was assessed using scanning electron microscopy (SEM) coupled with energy dispersive X-ray (EDX) analysis after decay of the 68Ga- and 99mTc-labelled carbon nanoparticles, respectively.
32886302	1	57	theme	68Ga-labelled	144:156	arg1	nanoparticles					165:177	68Ga-labelled carbon nanoparticles	144:177	68Ga-labelled carbon nanoparticles	144:177	PURPOSE The use of 68Ga-labelled carbon nanoparticles has been proposed for lung ventilation PET/CT imaging.
32886302	9	58	theme	usual	1552:1556	arg1	way					1567:1569	the usual clinical way	1548:1569	the usual clinical way	1548:1569	CONCLUSION Using Technegas® generator in the usual clinical way, 99mTc- and 68Ga-labelled carbon nanoparticles demonstrated similar shape and diameters in the same size range size.
32886302	6	59	theme	99Tc-labelled	976:988	arg1	nanoparticles					997:1009	99Tc-labelled carbon nanoparticles	976:1009	99Tc-labelled carbon nanoparticles	976:1009	The composition of the 68Zn- and 99Tc-labelled carbon nanoparticles aerosols was assessed using scanning electron microscopy (SEM) coupled with energy dispersive X-ray (EDX) analysis after decay of the 68Ga- and 99mTc-labelled carbon nanoparticles, respectively.
32886302	4	60	theme	HCl-based	658:666	arg1	nanoparticles					675:687	0.1 N HCl-based carbon nanoparticles	652:687	0.1 N HCl-based carbon nanoparticles	652:687	PROCEDURES 99mTc- and 68Ga-labelled carbon nanoparticles, stable gallium carbon nanoparticles, 0.9 % NaCl and 0.1 N HCl-based carbon nanoparticles were produced using an unmodified Technegas® generator, following the usual technique used for clinical Technegas® production.
32886302	6	61	theme	68Zn-	966:970	arg1	composition					947:957	The composition	943:957	The composition of the 68Zn- and 99Tc-labelled carbon nanoparticles aerosols	943:1018	The composition of the 68Zn- and 99Tc-labelled carbon nanoparticles aerosols was assessed using scanning electron microscopy (SEM) coupled with energy dispersive X-ray (EDX) analysis after decay of the 68Ga- and 99mTc-labelled carbon nanoparticles, respectively.
32886302	2	62	theme	physical	269:276	arg1	properties					278:287	the physical properties	265:287	the physical properties of 68Ga-labelled carbon nanoparticles	265:325	However, no study has assessed the physical properties of 68Ga-labelled carbon nanoparticles.
32886302	9	63	theme	99mTc-	1572:1577	arg1	nanoparticles					1604:1616	99mTc- and 68Ga-labelled carbon nanoparticles	1572:1616	99mTc- and 68Ga-labelled carbon nanoparticles	1572:1616	CONCLUSION Using Technegas® generator in the usual clinical way, 99mTc- and 68Ga-labelled carbon nanoparticles demonstrated similar shape and diameters in the same size range size.
32886302	9	64	dep	shape	1639:1643	arg1	size					1682:1685	size	1682:1685	size	1682:1685	CONCLUSION Using Technegas® generator in the usual clinical way, 99mTc- and 68Ga-labelled carbon nanoparticles demonstrated similar shape and diameters in the same size range size.
32886302	5	65	theme	transmission	864:875	arg1	microscopy					886:895	transmission electron microscopy	864:895	transmission electron microscopy (TEM)	864:901	The shape and size of particles were studied by transmission electron microscopy (TEM) after decay of the radioactive samples.
32886302	5	65	theme	transmission	864:875	arg1	TEM					898:900	TEM	898:900	TEM	898:900	The shape and size of particles were studied by transmission electron microscopy (TEM) after decay of the radioactive samples.
32886302	9	66	theme	68Ga-labelled	1583:1595	arg1	nanoparticles					1604:1616	99mTc- and 68Ga-labelled carbon nanoparticles	1572:1616	99mTc- and 68Ga-labelled carbon nanoparticles	1572:1616	CONCLUSION Using Technegas® generator in the usual clinical way, 99mTc- and 68Ga-labelled carbon nanoparticles demonstrated similar shape and diameters in the same size range size.
32886302	9	67	theme	same	1666:1669	arg1	range					1676:1680	the same size range	1662:1680	the same size range	1662:1680	CONCLUSION Using Technegas® generator in the usual clinical way, 99mTc- and 68Ga-labelled carbon nanoparticles demonstrated similar shape and diameters in the same size range size.
32886302	8	68	theme	primary	1350:1356	arg1	nanoparticles					1380:1392	primary stable gallium carbon nanoparticles	1350:1392	primary stable gallium carbon nanoparticles	1350:1392	The mean diameters of primary stable gallium carbon nanoparticles, 99Tc- and 68Zn-labelled carbon nanoparticles were 22.4 ± 10 nm, 20.9 ± 7.2 nm and 19.8 ± 11.7 nm, respectively.
32886302	7	69	theme	similar	1241:1247	arg1	shape					1249:1253	similar shape	1241:1253	similar shape	1241:1253	RESULTS On TEM, all samples showed similar shape with hexagonally structured primary particles, agglomerated in clusters.
32886302	1	70	theme	nanoparticles	165:177	arg1	use					137:139	The use	133:139	The use of 68Ga-labelled carbon nanoparticles	133:177	PURPOSE The use of 68Ga-labelled carbon nanoparticles has been proposed for lung ventilation PET/CT imaging.
32886302	9	71	from	diameters	1649:1657	arg1	range					1676:1680	the same size range	1662:1680	the same size range	1662:1680	CONCLUSION Using Technegas® generator in the usual clinical way, 99mTc- and 68Ga-labelled carbon nanoparticles demonstrated similar shape and diameters in the same size range size.
32886302	6	72	theme	electron	1048:1055	arg1	SEM					1069:1071	SEM	1069:1071	SEM	1069:1071	The composition of the 68Zn- and 99Tc-labelled carbon nanoparticles aerosols was assessed using scanning electron microscopy (SEM) coupled with energy dispersive X-ray (EDX) analysis after decay of the 68Ga- and 99mTc-labelled carbon nanoparticles, respectively.
32886302	6	72	theme	electron	1048:1055	arg1	microscopy					1057:1066	scanning electron microscopy	1039:1066	scanning electron microscopy (SEM) coupled with energy dispersive X-ray (EDX) analysis after decay of the 68Ga- and 99mTc-labelled carbon nanoparticles	1039:1189	The composition of the 68Zn- and 99Tc-labelled carbon nanoparticles aerosols was assessed using scanning electron microscopy (SEM) coupled with energy dispersive X-ray (EDX) analysis after decay of the 68Ga- and 99mTc-labelled carbon nanoparticles, respectively.
32886302	7	73	dep	RESULTS	1206:1212	arg1	showed					1234:1239	showed	1234:1239	showed similar shape with hexagonally structured primary particles, agglomerated in clusters	1234:1325	RESULTS On TEM, all samples showed similar shape with hexagonally structured primary particles, agglomerated in clusters.
32886302	4	74	theme	Technegas®	723:732	arg1	generator					734:742	an unmodified Technegas® generator	709:742	an unmodified Technegas® generator	709:742	PROCEDURES 99mTc- and 68Ga-labelled carbon nanoparticles, stable gallium carbon nanoparticles, 0.9 % NaCl and 0.1 N HCl-based carbon nanoparticles were produced using an unmodified Technegas® generator, following the usual technique used for clinical Technegas® production.
32886302	9	75	theme	similar	1631:1637	arg1	shape					1639:1643	similar shape	1631:1643	similar shape	1631:1643	CONCLUSION Using Technegas® generator in the usual clinical way, 99mTc- and 68Ga-labelled carbon nanoparticles demonstrated similar shape and diameters in the same size range size.
32886302	9	76	from	shape	1639:1643	arg1	range					1676:1680	the same size range	1662:1680	the same size range	1662:1680	CONCLUSION Using Technegas® generator in the usual clinical way, 99mTc- and 68Ga-labelled carbon nanoparticles demonstrated similar shape and diameters in the same size range size.
32886302	8	77	theme	nanoparticles	1380:1392	arg1	diameters					1337:1345	The mean diameters	1328:1345	The mean diameters of primary stable gallium carbon nanoparticles, 99Tc- and 68Zn-labelled carbon nanoparticles	1328:1438	The mean diameters of primary stable gallium carbon nanoparticles, 99Tc- and 68Zn-labelled carbon nanoparticles were 22.4 ± 10 nm, 20.9 ± 7.2 nm and 19.8 ± 11.7 nm, respectively.
32886302	8	77	theme	nanoparticles	1380:1392	arg1	22.4 ± 10 nm					1445:1456	22.4 ± 10 nm	1445:1456	22.4 ± 10 nm	1445:1456	The mean diameters of primary stable gallium carbon nanoparticles, 99Tc- and 68Zn-labelled carbon nanoparticles were 22.4 ± 10 nm, 20.9 ± 7.2 nm and 19.8 ± 11.7 nm, respectively.
32886302	4	78	theme	Technegas®	793:802	arg1	production					804:813	clinical Technegas® production	784:813	clinical Technegas® production	784:813	PROCEDURES 99mTc- and 68Ga-labelled carbon nanoparticles, stable gallium carbon nanoparticles, 0.9 % NaCl and 0.1 N HCl-based carbon nanoparticles were produced using an unmodified Technegas® generator, following the usual technique used for clinical Technegas® production.
32886302	1	79	theme	PET/CT	218:223	arg1	imaging					225:231	lung ventilation PET/CT imaging	201:231	lung ventilation PET/CT imaging	201:231	PURPOSE The use of 68Ga-labelled carbon nanoparticles has been proposed for lung ventilation PET/CT imaging.
32886302	8	80	theme	99Tc-	1395:1399	arg1	diameters					1337:1345	The mean diameters	1328:1345	The mean diameters of primary stable gallium carbon nanoparticles, 99Tc- and 68Zn-labelled carbon nanoparticles	1328:1438	The mean diameters of primary stable gallium carbon nanoparticles, 99Tc- and 68Zn-labelled carbon nanoparticles were 22.4 ± 10 nm, 20.9 ± 7.2 nm and 19.8 ± 11.7 nm, respectively.
32886302	8	80	theme	99Tc-	1395:1399	arg1	22.4 ± 10 nm					1445:1456	22.4 ± 10 nm	1445:1456	22.4 ± 10 nm	1445:1456	The mean diameters of primary stable gallium carbon nanoparticles, 99Tc- and 68Zn-labelled carbon nanoparticles were 22.4 ± 10 nm, 20.9 ± 7.2 nm and 19.8 ± 11.7 nm, respectively.
32886302	10	81	theme	carbon	1735:1740	arg1	nanoparticles					1742:1754	68Ga-labelled carbon nanoparticles	1721:1754	68Ga-labelled carbon nanoparticles	1721:1754	These results support the use of 68Ga-labelled carbon nanoparticles for the assessment of regional lung ventilation function with PET imaging.
32886302	5	82	theme	particles	838:846	arg1	size					830:833	size	830:833	size	830:833	The shape and size of particles were studied by transmission electron microscopy (TEM) after decay of the radioactive samples.
32886302	5	82	theme	particles	838:846	arg1	shape					820:824	shape	820:824	shape	820:824	The shape and size of particles were studied by transmission electron microscopy (TEM) after decay of the radioactive samples.
32886302	4	83	theme	carbon	578:583	arg1	nanoparticles					585:597	68Ga-labelled carbon nanoparticles	564:597	68Ga-labelled carbon nanoparticles	564:597	PROCEDURES 99mTc- and 68Ga-labelled carbon nanoparticles, stable gallium carbon nanoparticles, 0.9 % NaCl and 0.1 N HCl-based carbon nanoparticles were produced using an unmodified Technegas® generator, following the usual technique used for clinical Technegas® production.
32886302	8	84	theme	68Zn-labelled	1405:1417	arg1	nanoparticles					1426:1438	68Zn-labelled carbon nanoparticles	1405:1438	68Zn-labelled carbon nanoparticles	1405:1438	The mean diameters of primary stable gallium carbon nanoparticles, 99Tc- and 68Zn-labelled carbon nanoparticles were 22.4 ± 10 nm, 20.9 ± 7.2 nm and 19.8 ± 11.7 nm, respectively.
32886302	0	85	dep	Properties	76:85	arg1	Study					87:91	Study	87:91	Study	87:91	68Ga-Labelled Carbon Nanoparticles for Ventilation PET/CT Imaging: Physical Properties Study and Comparison with Technegas®.
32886302	0	85	dep	Properties	76:85	arg1	Properties					76:85	Imaging: Physical Properties	58:85	Imaging: Physical Properties Study and Comparison with Technegas®	58:122	68Ga-Labelled Carbon Nanoparticles for Ventilation PET/CT Imaging: Physical Properties Study and Comparison with Technegas®.
32886302	0	85	dep	Properties	76:85	arg1	Comparison					97:106	Comparison	97:106	Comparison with Technegas®	97:122	68Ga-Labelled Carbon Nanoparticles for Ventilation PET/CT Imaging: Physical Properties Study and Comparison with Technegas®.
32886302	6	86	theme	dispersive	1094:1103	arg1	analysis					1117:1124	energy dispersive X-ray (EDX) analysis	1087:1124	energy dispersive X-ray (EDX) analysis after decay of the 68Ga- and 99mTc-labelled carbon nanoparticles	1087:1189	The composition of the 68Zn- and 99Tc-labelled carbon nanoparticles aerosols was assessed using scanning electron microscopy (SEM) coupled with energy dispersive X-ray (EDX) analysis after decay of the 68Ga- and 99mTc-labelled carbon nanoparticles, respectively.
32886302	8	87	theme	nanoparticles	1426:1438	arg1	diameters					1337:1345	The mean diameters	1328:1345	The mean diameters of primary stable gallium carbon nanoparticles, 99Tc- and 68Zn-labelled carbon nanoparticles	1328:1438	The mean diameters of primary stable gallium carbon nanoparticles, 99Tc- and 68Zn-labelled carbon nanoparticles were 22.4 ± 10 nm, 20.9 ± 7.2 nm and 19.8 ± 11.7 nm, respectively.
32886302	8	87	theme	nanoparticles	1426:1438	arg1	22.4 ± 10 nm					1445:1456	22.4 ± 10 nm	1445:1456	22.4 ± 10 nm	1445:1456	The mean diameters of primary stable gallium carbon nanoparticles, 99Tc- and 68Zn-labelled carbon nanoparticles were 22.4 ± 10 nm, 20.9 ± 7.2 nm and 19.8 ± 11.7 nm, respectively.
32886302	3	88	theme	aerosol	474:480	arg1	composition					455:465	the composition	451:465	the composition of the aerosol	451:480	The aim of this study therefore was to evaluate the shape and size of 68Ga-labelled carbon nanoparticles, and to determine the composition of the aerosol, as opposed to 99mTc-labelled carbon nanoparticles aerosol.
32886302	6	89	theme	99mTc-labelled	1155:1168	arg1	nanoparticles					1177:1189	the 68Ga- and 99mTc-labelled carbon nanoparticles	1141:1189	the 68Ga- and 99mTc-labelled carbon nanoparticles	1141:1189	The composition of the 68Zn- and 99Tc-labelled carbon nanoparticles aerosols was assessed using scanning electron microscopy (SEM) coupled with energy dispersive X-ray (EDX) analysis after decay of the 68Ga- and 99mTc-labelled carbon nanoparticles, respectively.
32886302	3	90	theme	carbon	412:417	arg1	nanoparticles					419:431	68Ga-labelled carbon nanoparticles	398:431	68Ga-labelled carbon nanoparticles	398:431	The aim of this study therefore was to evaluate the shape and size of 68Ga-labelled carbon nanoparticles, and to determine the composition of the aerosol, as opposed to 99mTc-labelled carbon nanoparticles aerosol.
32886302	10	91	theme	ventilation	1792:1802	arg1	assessment					1764:1773	the assessment	1760:1773	the assessment of regional lung ventilation	1760:1802	These results support the use of 68Ga-labelled carbon nanoparticles for the assessment of regional lung ventilation function with PET imaging.
32886302	6	92	theme	68Ga-	1145:1149	arg1	nanoparticles					1177:1189	the 68Ga- and 99mTc-labelled carbon nanoparticles	1141:1189	the 68Ga- and 99mTc-labelled carbon nanoparticles	1141:1189	The composition of the 68Zn- and 99Tc-labelled carbon nanoparticles aerosols was assessed using scanning electron microscopy (SEM) coupled with energy dispersive X-ray (EDX) analysis after decay of the 68Ga- and 99mTc-labelled carbon nanoparticles, respectively.
32886302	4	93	theme	0.9 	637:640	arg1	%					641:641	%	641:641	%	641:641	PROCEDURES 99mTc- and 68Ga-labelled carbon nanoparticles, stable gallium carbon nanoparticles, 0.9 % NaCl and 0.1 N HCl-based carbon nanoparticles were produced using an unmodified Technegas® generator, following the usual technique used for clinical Technegas® production.
32886302	10	94	theme	regional	1778:1785	arg1	ventilation					1792:1802	regional lung ventilation	1778:1802	regional lung ventilation	1778:1802	These results support the use of 68Ga-labelled carbon nanoparticles for the assessment of regional lung ventilation function with PET imaging.
32886302	10	95	theme	PET	1818:1820	arg1	imaging					1822:1828	PET imaging	1818:1828	PET imaging	1818:1828	These results support the use of 68Ga-labelled carbon nanoparticles for the assessment of regional lung ventilation function with PET imaging.
32886302	0	96	theme	Carbon	14:19	arg1	Nanoparticles					21:33	68Ga-Labelled Carbon Nanoparticles	0:33	68Ga-Labelled Carbon Nanoparticles for Ventilation	0:49	68Ga-Labelled Carbon Nanoparticles for Ventilation PET/CT Imaging: Physical Properties Study and Comparison with Technegas®.
33233058	9	0	theme	Bifidobacterium	1373:1387	arg1	growth					1389:1394	Bifidobacterium growth	1373:1394	Bifidobacterium growth	1373:1394	FVBP flour showed higher prebiotic effect than FOS on growth enhancement of Lactobacillus after 48 h of fermentation and similar bifidogenic effect as FOS on Bifidobacterium growth at 12, 24 and 48 h of fermentation.
33233058	10	1	theme	flour	1571:1575	arg1	potential					1553:1561	the prebiotic potential	1539:1561	the prebiotic potential of this flour	1539:1575	SCFA production was observed when FVBP flour was used as carbon source, including butyrate, which supports the prebiotic potential of this flour.
33233058	8	2	theme	cell	1180:1183	arg1	viability					1185:1193	cell viability	1180:1193	cell viability	1180:1193	The impact of FVBP flour upon cell viability was also evaluated.
33233058	10	3	theme	carbon	1489:1494	arg1	source					1496:1501	carbon source	1489:1501	carbon source	1489:1501	SCFA production was observed when FVBP flour was used as carbon source, including butyrate, which supports the prebiotic potential of this flour.
33233058	10	3	theme	carbon	1489:1494	arg1	flour					1471:1475	FVBP flour	1466:1475	FVBP flour	1466:1475	SCFA production was observed when FVBP flour was used as carbon source, including butyrate, which supports the prebiotic potential of this flour.
33233058	12	4	theme	prebiotic	1859:1867	arg1	flour					1826:1830	a fruit and vegetable byproducts flour	1793:1830	a fruit and vegetable byproducts flour	1793:1830	Thus, the present study demonstrates the viability of using a fruit and vegetable byproducts flour as a potential sustainable prebiotic source.
33233058	12	4	theme	prebiotic	1859:1867	arg1	source					1869:1874	a potential sustainable prebiotic source	1835:1874	a potential sustainable prebiotic source	1835:1874	Thus, the present study demonstrates the viability of using a fruit and vegetable byproducts flour as a potential sustainable prebiotic source.
33233058	10	5	used	used	1481:1484	arg2	flour					1471:1475	FVBP flour	1466:1475	FVBP flour	1466:1475	SCFA production was observed when FVBP flour was used as carbon source, including butyrate, which supports the prebiotic potential of this flour.
33233058	10	5	used	used	1481:1484	arg2	source					1496:1501	carbon source	1489:1501	carbon source	1489:1501	SCFA production was observed when FVBP flour was used as carbon source, including butyrate, which supports the prebiotic potential of this flour.
33233058	1	6	theme	microbiota	271:280	arg1	composition					282:292	gut microbiota composition	267:292	gut microbiota composition	267:292	Fruit and vegetable byproducts (FVBP) present high content of bioactive compounds and dietary fibers and have demonstrated a positive modulatory effect upon gut microbiota composition.
33233058	11	7	theme	cell	1712:1715	arg1	line					1717:1720	Caco-2 cell line	1705:1720	Caco-2 cell line	1705:1720	Additionally, it was observed that after in vitro gastrointestinal digestion, the FVBP flour at 3% promoted cell metabolism of Caco-2 cell line up to 67%.
33233058	1	8	theme	compounds	182:190	arg1	content					161:167	high content	156:167	high content of bioactive compounds and dietary fibers	156:209	Fruit and vegetable byproducts (FVBP) present high content of bioactive compounds and dietary fibers and have demonstrated a positive modulatory effect upon gut microbiota composition.
33233058	9	9	from	effect	1250:1255	arg1	growth					1389:1394	Bifidobacterium growth	1373:1394	Bifidobacterium growth	1373:1394	FVBP flour showed higher prebiotic effect than FOS on growth enhancement of Lactobacillus after 48 h of fermentation and similar bifidogenic effect as FOS on Bifidobacterium growth at 12, 24 and 48 h of fermentation.
33233058	9	9	from	effect	1250:1255	arg1	enhancement					1276:1286	growth enhancement	1269:1286	growth enhancement of Lactobacillus after 48 h of fermentation	1269:1330	FVBP flour showed higher prebiotic effect than FOS on growth enhancement of Lactobacillus after 48 h of fermentation and similar bifidogenic effect as FOS on Bifidobacterium growth at 12, 24 and 48 h of fermentation.
33233058	11	10	from	%	1675:1675	arg1	flour					1665:1669	the FVBP flour	1656:1669	the FVBP flour at 3%	1656:1675	Additionally, it was observed that after in vitro gastrointestinal digestion, the FVBP flour at 3% promoted cell metabolism of Caco-2 cell line up to 67%.
33233058	5	11	theme	polymerase	914:923	arg1	qPCR					941:944	qPCR	941:944	qPCR	941:944	The changes in gut microbiota were evaluated at 0, 12, 24 and 48 h of fermentation by the real-time polymerase chain reaction (qPCR) method with 16S rRNA-based specific primers.
33233058	5	11	theme	polymerase	914:923	arg1	reaction					931:938	the real-time polymerase chain reaction	900:938	the real-time polymerase chain reaction (qPCR) method with 16S rRNA-based specific primers	900:989	The changes in gut microbiota were evaluated at 0, 12, 24 and 48 h of fermentation by the real-time polymerase chain reaction (qPCR) method with 16S rRNA-based specific primers.
33233058	3	12	theme	Lactobacillus	564:576	arg1	LA-5®					622:626	Lactobacillus casei 01, Lactobacillus rhamnosus R11 and Lactobacillus acidophilus LA-5®	540:626	Lactobacillus casei 01, Lactobacillus rhamnosus R11 and Lactobacillus acidophilus LA-5®	540:626	An initial screening with three strains of Lactobacillus (Lactobacillus casei 01, Lactobacillus rhamnosus R11 and Lactobacillus acidophilus LA-5®) and one Bifidobacterium strain (Bifidobacterium animalis spp.
33233058	3	12	theme	Lactobacillus	564:576	arg1	Lactobacillus					525:537	Lactobacillus	525:537	Lactobacillus (Lactobacillus casei 01, Lactobacillus rhamnosus R11 and Lactobacillus acidophilus LA-5®)	525:627	An initial screening with three strains of Lactobacillus (Lactobacillus casei 01, Lactobacillus rhamnosus R11 and Lactobacillus acidophilus LA-5®) and one Bifidobacterium strain (Bifidobacterium animalis spp.
33233058	1	13	theme	dietary	196:202	arg1	fibers					204:209	dietary fibers	196:209	dietary fibers	196:209	Fruit and vegetable byproducts (FVBP) present high content of bioactive compounds and dietary fibers and have demonstrated a positive modulatory effect upon gut microbiota composition.
33233058	1	14	theme	vegetable	120:128	arg1	byproducts					130:139	Fruit and vegetable byproducts	110:139	Fruit and vegetable byproducts (FVBP)	110:146	Fruit and vegetable byproducts (FVBP) present high content of bioactive compounds and dietary fibers and have demonstrated a positive modulatory effect upon gut microbiota composition.
33233058	1	14	theme	vegetable	120:128	arg1	FVBP					142:145	FVBP	142:145	FVBP	142:145	Fruit and vegetable byproducts (FVBP) present high content of bioactive compounds and dietary fibers and have demonstrated a positive modulatory effect upon gut microbiota composition.
33233058	5	15	with	method	947:952	arg1	primers					983:989	16S rRNA-based specific primers	959:989	16S rRNA-based specific primers	959:989	The changes in gut microbiota were evaluated at 0, 12, 24 and 48 h of fermentation by the real-time polymerase chain reaction (qPCR) method with 16S rRNA-based specific primers.
33233058	8	16	theme	flour	1169:1173	arg1	impact					1154:1159	The impact	1150:1159	The impact of FVBP flour upon cell viability	1150:1193	The impact of FVBP flour upon cell viability was also evaluated.
33233058	5	17	theme	fermentation	884:895	arg1	fermentation					884:895	fermentation	884:895	fermentation	884:895	The changes in gut microbiota were evaluated at 0, 12, 24 and 48 h of fermentation by the real-time polymerase chain reaction (qPCR) method with 16S rRNA-based specific primers.
33233058	5	17	theme	fermentation	884:895	arg1	24					869:870	24	869:870	24	869:870	The changes in gut microbiota were evaluated at 0, 12, 24 and 48 h of fermentation by the real-time polymerase chain reaction (qPCR) method with 16S rRNA-based specific primers.
33233058	4	18	theme	prebiotic	734:742	arg1	effect					744:749	then the prebiotic effect	725:749	then the prebiotic effect of FVBP flour	725:763	lactis BB12®) was carried out and then the prebiotic effect of FVBP flour was performed with fecal samples of five donors.
33233058	3	19	theme	Bifidobacterium	637:651	arg1	strain					653:658	one Bifidobacterium strain	633:658	one Bifidobacterium strain	633:658	An initial screening with three strains of Lactobacillus (Lactobacillus casei 01, Lactobacillus rhamnosus R11 and Lactobacillus acidophilus LA-5®) and one Bifidobacterium strain (Bifidobacterium animalis spp.
33233058	11	20	theme	FVBP	1660:1663	arg1	flour					1665:1669	the FVBP flour	1656:1669	the FVBP flour at 3%	1656:1675	Additionally, it was observed that after in vitro gastrointestinal digestion, the FVBP flour at 3% promoted cell metabolism of Caco-2 cell line up to 67%.
33233058	0	21	theme	flour	61:65	arg1	effect					20:25	Potential prebiotic effect	0:25	Potential prebiotic effect of fruit and vegetable byproducts flour using in vitro gastrointestinal digestion.	0:108	Potential prebiotic effect of fruit and vegetable byproducts flour using in vitro gastrointestinal digestion.
33233058	3	22	dep	Lactobacillus	564:576	arg1	rhamnosus					578:586	Lactobacillus rhamnosus R11	564:590	Lactobacillus rhamnosus R11	564:590	An initial screening with three strains of Lactobacillus (Lactobacillus casei 01, Lactobacillus rhamnosus R11 and Lactobacillus acidophilus LA-5®) and one Bifidobacterium strain (Bifidobacterium animalis spp.
33233058	3	22	dep	Lactobacillus	564:576	arg1	R11					588:590	R11	588:590	R11	588:590	An initial screening with three strains of Lactobacillus (Lactobacillus casei 01, Lactobacillus rhamnosus R11 and Lactobacillus acidophilus LA-5®) and one Bifidobacterium strain (Bifidobacterium animalis spp.
33233058	11	23	theme	cell	1686:1689	arg1	metabolism					1691:1700	cell metabolism	1686:1700	cell metabolism of Caco-2 cell line	1686:1720	Additionally, it was observed that after in vitro gastrointestinal digestion, the FVBP flour at 3% promoted cell metabolism of Caco-2 cell line up to 67%.
33233058	0	24	theme	in	73:74	arg1	digestion					99:107	in vitro gastrointestinal digestion	73:107	in vitro gastrointestinal digestion	73:107	Potential prebiotic effect of fruit and vegetable byproducts flour using in vitro gastrointestinal digestion.
33233058	9	25	theme	higher	1233:1238	arg1	effect					1250:1255	higher prebiotic effect	1233:1255	higher prebiotic effect than FOS on growth enhancement of Lactobacillus after 48 h of fermentation	1233:1330	FVBP flour showed higher prebiotic effect than FOS on growth enhancement of Lactobacillus after 48 h of fermentation and similar bifidogenic effect as FOS on Bifidobacterium growth at 12, 24 and 48 h of fermentation.
33233058	12	26	theme	fruit	1795:1799	arg1	byproducts					1815:1824	fruit and vegetable byproducts	1795:1824	a fruit and vegetable byproducts flour	1793:1830	Thus, the present study demonstrates the viability of using a fruit and vegetable byproducts flour as a potential sustainable prebiotic source.
33233058	1	27	theme	high	156:159	arg1	content					161:167	high content	156:167	high content of bioactive compounds and dietary fibers	156:209	Fruit and vegetable byproducts (FVBP) present high content of bioactive compounds and dietary fibers and have demonstrated a positive modulatory effect upon gut microbiota composition.
33233058	5	28	theme	reaction	931:938	arg1	method					947:952	the real-time polymerase chain reaction (qPCR) method	900:952	the real-time polymerase chain reaction (qPCR) method with 16S rRNA-based specific primers	900:989	The changes in gut microbiota were evaluated at 0, 12, 24 and 48 h of fermentation by the real-time polymerase chain reaction (qPCR) method with 16S rRNA-based specific primers.
33233058	0	29	theme	gastrointestinal	82:97	arg1	digestion					99:107	in vitro gastrointestinal digestion	73:107	in vitro gastrointestinal digestion	73:107	Potential prebiotic effect of fruit and vegetable byproducts flour using in vitro gastrointestinal digestion.
33233058	5	30	from	changes	818:824	arg1	microbiota					833:842	gut microbiota	829:842	gut microbiota	829:842	The changes in gut microbiota were evaluated at 0, 12, 24 and 48 h of fermentation by the real-time polymerase chain reaction (qPCR) method with 16S rRNA-based specific primers.
33233058	12	31	theme	vegetable	1805:1813	arg1	byproducts					1815:1824	fruit and vegetable byproducts	1795:1824	a fruit and vegetable byproducts flour	1793:1830	Thus, the present study demonstrates the viability of using a fruit and vegetable byproducts flour as a potential sustainable prebiotic source.
33233058	4	32	theme	fecal	784:788	arg1	samples					790:796	fecal samples	784:796	fecal samples of five donors	784:811	lactis BB12®) was carried out and then the prebiotic effect of FVBP flour was performed with fecal samples of five donors.
33233058	1	33	theme	gut	267:269	arg1	composition					282:292	gut microbiota composition	267:292	gut microbiota composition	267:292	Fruit and vegetable byproducts (FVBP) present high content of bioactive compounds and dietary fibers and have demonstrated a positive modulatory effect upon gut microbiota composition.
33233058	2	34	theme	in	445:446	arg1	digestion					471:479	in vitro gastrointestinal digestion	445:479	in vitro gastrointestinal digestion	445:479	In the present study, the prebiotic potential of a FVBP flour obtained from solid byproducts after fruit and vegetable processing was evaluated after in vitro gastrointestinal digestion.
33233058	2	35	theme	solid	371:375	arg1	byproducts					377:386	solid byproducts	371:386	solid byproducts	371:386	In the present study, the prebiotic potential of a FVBP flour obtained from solid byproducts after fruit and vegetable processing was evaluated after in vitro gastrointestinal digestion.
33233058	0	36	theme	Potential	0:8	arg1	effect					20:25	Potential prebiotic effect	0:25	Potential prebiotic effect of fruit and vegetable byproducts flour using in vitro gastrointestinal digestion.	0:108	Potential prebiotic effect of fruit and vegetable byproducts flour using in vitro gastrointestinal digestion.
33233058	9	37	theme	growth	1269:1274	arg1	enhancement					1276:1286	growth enhancement	1269:1286	growth enhancement of Lactobacillus after 48 h of fermentation	1269:1330	FVBP flour showed higher prebiotic effect than FOS on growth enhancement of Lactobacillus after 48 h of fermentation and similar bifidogenic effect as FOS on Bifidobacterium growth at 12, 24 and 48 h of fermentation.
33233058	0	38	dep	in	73:74	arg1	vitro					76:80	vitro	76:80	vitro	76:80	Potential prebiotic effect of fruit and vegetable byproducts flour using in vitro gastrointestinal digestion.
33233058	6	39	theme	acids	1021:1025	arg1	production					1034:1043	short chain fatty acids (SCFA) production	1003:1043	short chain fatty acids (SCFA) production	1003:1043	The pH and short chain fatty acids (SCFA) production at each fermentation time were assessed.
33233058	4	40	theme	flour	759:763	arg1	effect					744:749	then the prebiotic effect	725:749	then the prebiotic effect of FVBP flour	725:763	lactis BB12®) was carried out and then the prebiotic effect of FVBP flour was performed with fecal samples of five donors.
33233058	12	41	theme	present	1743:1749	arg1	study					1751:1755	the present study	1739:1755	the present study	1739:1755	Thus, the present study demonstrates the viability of using a fruit and vegetable byproducts flour as a potential sustainable prebiotic source.
33233058	1	42	theme	Fruit	110:114	arg1	byproducts					130:139	Fruit and vegetable byproducts	110:139	Fruit and vegetable byproducts (FVBP)	110:146	Fruit and vegetable byproducts (FVBP) present high content of bioactive compounds and dietary fibers and have demonstrated a positive modulatory effect upon gut microbiota composition.
33233058	1	42	theme	Fruit	110:114	arg1	FVBP					142:145	FVBP	142:145	FVBP	142:145	Fruit and vegetable byproducts (FVBP) present high content of bioactive compounds and dietary fibers and have demonstrated a positive modulatory effect upon gut microbiota composition.
33233058	6	43	theme	chain	1009:1013	arg1	SCFA					1028:1031	SCFA	1028:1031	SCFA	1028:1031	The pH and short chain fatty acids (SCFA) production at each fermentation time were assessed.
33233058	6	43	theme	chain	1009:1013	arg1	acids					1021:1025	short chain fatty acids	1003:1025	short chain fatty acids (SCFA) production	1003:1043	The pH and short chain fatty acids (SCFA) production at each fermentation time were assessed.
33233058	0	44	theme	fruit	30:34	arg1	flour					61:65	fruit and vegetable byproducts flour	30:65	fruit and vegetable byproducts flour using in vitro gastrointestinal digestion	30:107	Potential prebiotic effect of fruit and vegetable byproducts flour using in vitro gastrointestinal digestion.
33233058	7	45	used	used	1124:1127	arg2	FOS					1114:1116	FOS	1114:1116	FOS	1114:1116	The fructooligosaccharides (FOS) were used as positive control.
33233058	7	45	used	used	1124:1127	arg2	control					1141:1147	positive control	1132:1147	positive control	1132:1147	The fructooligosaccharides (FOS) were used as positive control.
33233058	7	45	used	used	1124:1127	arg2	fructooligosaccharides					1090:1111	The fructooligosaccharides	1086:1111	The fructooligosaccharides (FOS)	1086:1117	The fructooligosaccharides (FOS) were used as positive control.
33233058	2	46	theme	gastrointestinal	454:469	arg1	digestion					471:479	in vitro gastrointestinal digestion	445:479	in vitro gastrointestinal digestion	445:479	In the present study, the prebiotic potential of a FVBP flour obtained from solid byproducts after fruit and vegetable processing was evaluated after in vitro gastrointestinal digestion.
33233058	10	47	theme	FVBP	1466:1469	arg1	source					1496:1501	carbon source	1489:1501	carbon source	1489:1501	SCFA production was observed when FVBP flour was used as carbon source, including butyrate, which supports the prebiotic potential of this flour.
33233058	10	47	theme	FVBP	1466:1469	arg1	flour					1471:1475	FVBP flour	1466:1475	FVBP flour	1466:1475	SCFA production was observed when FVBP flour was used as carbon source, including butyrate, which supports the prebiotic potential of this flour.
33233058	6	48	from	time	1066:1069	arg1	production					1034:1043	short chain fatty acids (SCFA) production	1003:1043	short chain fatty acids (SCFA) production	1003:1043	The pH and short chain fatty acids (SCFA) production at each fermentation time were assessed.
33233058	6	48	from	time	1066:1069	arg1	pH					996:997	pH	996:997	pH	996:997	The pH and short chain fatty acids (SCFA) production at each fermentation time were assessed.
33233058	0	49	theme	vegetable	40:48	arg1	flour					61:65	fruit and vegetable byproducts flour	30:65	fruit and vegetable byproducts flour using in vitro gastrointestinal digestion	30:107	Potential prebiotic effect of fruit and vegetable byproducts flour using in vitro gastrointestinal digestion.
33233058	9	50	from	effect	1356:1361	arg1	growth					1389:1394	Bifidobacterium growth	1373:1394	Bifidobacterium growth	1373:1394	FVBP flour showed higher prebiotic effect than FOS on growth enhancement of Lactobacillus after 48 h of fermentation and similar bifidogenic effect as FOS on Bifidobacterium growth at 12, 24 and 48 h of fermentation.
33233058	9	50	from	effect	1356:1361	arg1	enhancement					1276:1286	growth enhancement	1269:1286	growth enhancement of Lactobacillus after 48 h of fermentation	1269:1330	FVBP flour showed higher prebiotic effect than FOS on growth enhancement of Lactobacillus after 48 h of fermentation and similar bifidogenic effect as FOS on Bifidobacterium growth at 12, 24 and 48 h of fermentation.
33233058	2	51	theme	prebiotic	321:329	arg1	potential					331:339	the prebiotic potential	317:339	the prebiotic potential of a FVBP flour obtained from solid byproducts after fruit and vegetable processing	317:423	In the present study, the prebiotic potential of a FVBP flour obtained from solid byproducts after fruit and vegetable processing was evaluated after in vitro gastrointestinal digestion.
33233058	5	52	theme	16S	959:961	arg1	primers					983:989	16S rRNA-based specific primers	959:989	16S rRNA-based specific primers	959:989	The changes in gut microbiota were evaluated at 0, 12, 24 and 48 h of fermentation by the real-time polymerase chain reaction (qPCR) method with 16S rRNA-based specific primers.
33233058	4	53	theme	donors	806:811	arg1	samples					790:796	fecal samples	784:796	fecal samples of five donors	784:811	lactis BB12®) was carried out and then the prebiotic effect of FVBP flour was performed with fecal samples of five donors.
33233058	9	54	theme	bifidogenic	1344:1354	arg1	effect					1356:1361	similar bifidogenic effect	1336:1361	similar bifidogenic effect as FOS on Bifidobacterium growth	1336:1394	FVBP flour showed higher prebiotic effect than FOS on growth enhancement of Lactobacillus after 48 h of fermentation and similar bifidogenic effect as FOS on Bifidobacterium growth at 12, 24 and 48 h of fermentation.
33233058	5	55	theme	specific	974:981	arg1	primers					983:989	16S rRNA-based specific primers	959:989	16S rRNA-based specific primers	959:989	The changes in gut microbiota were evaluated at 0, 12, 24 and 48 h of fermentation by the real-time polymerase chain reaction (qPCR) method with 16S rRNA-based specific primers.
33233058	2	56	theme	FVBP	346:349	arg1	flour					351:355	a FVBP flour	344:355	a FVBP flour obtained from solid byproducts after fruit and vegetable processing	344:423	In the present study, the prebiotic potential of a FVBP flour obtained from solid byproducts after fruit and vegetable processing was evaluated after in vitro gastrointestinal digestion.
33233058	1	57	theme	modulatory	244:253	arg1	effect					255:260	a positive modulatory effect	233:260	a positive modulatory effect upon gut microbiota composition	233:292	Fruit and vegetable byproducts (FVBP) present high content of bioactive compounds and dietary fibers and have demonstrated a positive modulatory effect upon gut microbiota composition.
33233058	3	58	theme	casei	554:558	arg1	LA-5®					622:626	Lactobacillus casei 01, Lactobacillus rhamnosus R11 and Lactobacillus acidophilus LA-5®	540:626	Lactobacillus casei 01, Lactobacillus rhamnosus R11 and Lactobacillus acidophilus LA-5®	540:626	An initial screening with three strains of Lactobacillus (Lactobacillus casei 01, Lactobacillus rhamnosus R11 and Lactobacillus acidophilus LA-5®) and one Bifidobacterium strain (Bifidobacterium animalis spp.
33233058	3	58	theme	casei	554:558	arg1	Lactobacillus					525:537	Lactobacillus	525:537	Lactobacillus (Lactobacillus casei 01, Lactobacillus rhamnosus R11 and Lactobacillus acidophilus LA-5®)	525:627	An initial screening with three strains of Lactobacillus (Lactobacillus casei 01, Lactobacillus rhamnosus R11 and Lactobacillus acidophilus LA-5®) and one Bifidobacterium strain (Bifidobacterium animalis spp.
33233058	3	59	with	screening	493:501	arg1	strains					514:520	three strains	508:520	three strains of Lactobacillus (Lactobacillus casei 01, Lactobacillus rhamnosus R11 and Lactobacillus acidophilus LA-5®)	508:627	An initial screening with three strains of Lactobacillus (Lactobacillus casei 01, Lactobacillus rhamnosus R11 and Lactobacillus acidophilus LA-5®) and one Bifidobacterium strain (Bifidobacterium animalis spp.
33233058	3	59	with	screening	493:501	arg1	strain					653:658	one Bifidobacterium strain	633:658	one Bifidobacterium strain	633:658	An initial screening with three strains of Lactobacillus (Lactobacillus casei 01, Lactobacillus rhamnosus R11 and Lactobacillus acidophilus LA-5®) and one Bifidobacterium strain (Bifidobacterium animalis spp.
33233058	1	60	theme	bioactive	172:180	arg1	compounds					182:190	bioactive compounds	172:190	bioactive compounds	172:190	Fruit and vegetable byproducts (FVBP) present high content of bioactive compounds and dietary fibers and have demonstrated a positive modulatory effect upon gut microbiota composition.
33233058	12	61	theme	sustainable	1847:1857	arg1	flour					1826:1830	a fruit and vegetable byproducts flour	1793:1830	a fruit and vegetable byproducts flour	1793:1830	Thus, the present study demonstrates the viability of using a fruit and vegetable byproducts flour as a potential sustainable prebiotic source.
33233058	12	61	theme	sustainable	1847:1857	arg1	source					1869:1874	a potential sustainable prebiotic source	1835:1874	a potential sustainable prebiotic source	1835:1874	Thus, the present study demonstrates the viability of using a fruit and vegetable byproducts flour as a potential sustainable prebiotic source.
33233058	4	62	theme	lactis	691:696	arg1	BB12®					698:702	lactis BB12®)	691:703	lactis BB12®)	691:703	lactis BB12®) was carried out and then the prebiotic effect of FVBP flour was performed with fecal samples of five donors.
33233058	11	63	theme	line	1717:1720	arg1	metabolism					1691:1700	cell metabolism	1686:1700	cell metabolism of Caco-2 cell line	1686:1720	Additionally, it was observed that after in vitro gastrointestinal digestion, the FVBP flour at 3% promoted cell metabolism of Caco-2 cell line up to 67%.
33233058	2	64	theme	present	302:308	arg1	study					310:314	the present study	298:314	the present study	298:314	In the present study, the prebiotic potential of a FVBP flour obtained from solid byproducts after fruit and vegetable processing was evaluated after in vitro gastrointestinal digestion.
33233058	5	65	theme	gut	829:831	arg1	microbiota					833:842	gut microbiota	829:842	gut microbiota	829:842	The changes in gut microbiota were evaluated at 0, 12, 24 and 48 h of fermentation by the real-time polymerase chain reaction (qPCR) method with 16S rRNA-based specific primers.
33233058	11	66	theme	Caco-2	1705:1710	arg1	line					1717:1720	Caco-2 cell line	1705:1720	Caco-2 cell line	1705:1720	Additionally, it was observed that after in vitro gastrointestinal digestion, the FVBP flour at 3% promoted cell metabolism of Caco-2 cell line up to 67%.
33233058	3	67	theme	acidophilus	610:620	arg1	LA-5®					622:626	Lactobacillus casei 01, Lactobacillus rhamnosus R11 and Lactobacillus acidophilus LA-5®	540:626	Lactobacillus casei 01, Lactobacillus rhamnosus R11 and Lactobacillus acidophilus LA-5®	540:626	An initial screening with three strains of Lactobacillus (Lactobacillus casei 01, Lactobacillus rhamnosus R11 and Lactobacillus acidophilus LA-5®) and one Bifidobacterium strain (Bifidobacterium animalis spp.
33233058	3	67	theme	acidophilus	610:620	arg1	Lactobacillus					525:537	Lactobacillus	525:537	Lactobacillus (Lactobacillus casei 01, Lactobacillus rhamnosus R11 and Lactobacillus acidophilus LA-5®)	525:627	An initial screening with three strains of Lactobacillus (Lactobacillus casei 01, Lactobacillus rhamnosus R11 and Lactobacillus acidophilus LA-5®) and one Bifidobacterium strain (Bifidobacterium animalis spp.
33233058	1	68	theme	fibers	204:209	arg1	content					161:167	high content	156:167	high content of bioactive compounds and dietary fibers	156:209	Fruit and vegetable byproducts (FVBP) present high content of bioactive compounds and dietary fibers and have demonstrated a positive modulatory effect upon gut microbiota composition.
33233058	10	69	theme	prebiotic	1543:1551	arg1	potential					1553:1561	the prebiotic potential	1539:1561	the prebiotic potential of this flour	1539:1575	SCFA production was observed when FVBP flour was used as carbon source, including butyrate, which supports the prebiotic potential of this flour.
33233058	9	70	theme	fermentation	1418:1429	arg1	12					1399:1400	12	1399:1400	12	1399:1400	FVBP flour showed higher prebiotic effect than FOS on growth enhancement of Lactobacillus after 48 h of fermentation and similar bifidogenic effect as FOS on Bifidobacterium growth at 12, 24 and 48 h of fermentation.
33233058	9	70	theme	fermentation	1418:1429	arg1	fermentation					1418:1429	fermentation	1418:1429	fermentation	1418:1429	FVBP flour showed higher prebiotic effect than FOS on growth enhancement of Lactobacillus after 48 h of fermentation and similar bifidogenic effect as FOS on Bifidobacterium growth at 12, 24 and 48 h of fermentation.
33233058	8	71	theme	FVBP	1164:1167	arg1	flour					1169:1173	FVBP flour	1164:1173	FVBP flour	1164:1173	The impact of FVBP flour upon cell viability was also evaluated.
33233058	9	72	theme	FVBP	1215:1218	arg1	flour					1220:1224	FVBP flour	1215:1224	FVBP flour	1215:1224	FVBP flour showed higher prebiotic effect than FOS on growth enhancement of Lactobacillus after 48 h of fermentation and similar bifidogenic effect as FOS on Bifidobacterium growth at 12, 24 and 48 h of fermentation.
33233058	9	73	theme	prebiotic	1240:1248	arg1	effect					1250:1255	higher prebiotic effect	1233:1255	higher prebiotic effect than FOS on growth enhancement of Lactobacillus after 48 h of fermentation	1233:1330	FVBP flour showed higher prebiotic effect than FOS on growth enhancement of Lactobacillus after 48 h of fermentation and similar bifidogenic effect as FOS on Bifidobacterium growth at 12, 24 and 48 h of fermentation.
33233058	12	74	theme	byproducts	1815:1824	arg1	flour					1826:1830	a fruit and vegetable byproducts flour	1793:1830	a fruit and vegetable byproducts flour	1793:1830	Thus, the present study demonstrates the viability of using a fruit and vegetable byproducts flour as a potential sustainable prebiotic source.
33233058	12	74	theme	byproducts	1815:1824	arg1	source					1869:1874	a potential sustainable prebiotic source	1835:1874	a potential sustainable prebiotic source	1835:1874	Thus, the present study demonstrates the viability of using a fruit and vegetable byproducts flour as a potential sustainable prebiotic source.
33233058	3	75	theme	initial	485:491	arg1	screening					493:501	An initial screening	482:501	An initial screening with three strains of Lactobacillus (Lactobacillus casei 01, Lactobacillus rhamnosus R11 and Lactobacillus acidophilus LA-5®) and one Bifidobacterium strain (Bifidobacterium animalis	482:684	An initial screening with three strains of Lactobacillus (Lactobacillus casei 01, Lactobacillus rhamnosus R11 and Lactobacillus acidophilus LA-5®) and one Bifidobacterium strain (Bifidobacterium animalis spp.
33233058	7	76	theme	positive	1132:1139	arg1	control					1141:1147	positive control	1132:1147	positive control	1132:1147	The fructooligosaccharides (FOS) were used as positive control.
33233058	7	76	theme	positive	1132:1139	arg1	fructooligosaccharides					1090:1111	The fructooligosaccharides	1086:1111	The fructooligosaccharides (FOS)	1086:1117	The fructooligosaccharides (FOS) were used as positive control.
33233058	6	77	dep	pH	996:997	arg1	The					992:994	The	992:994	The	992:994	The pH and short chain fatty acids (SCFA) production at each fermentation time were assessed.
33233058	0	78	theme	prebiotic	10:18	arg1	effect					20:25	Potential prebiotic effect	0:25	Potential prebiotic effect of fruit and vegetable byproducts flour using in vitro gastrointestinal digestion.	0:108	Potential prebiotic effect of fruit and vegetable byproducts flour using in vitro gastrointestinal digestion.
33233058	2	79	dep	in	445:446	arg1	vitro					448:452	vitro	448:452	vitro	448:452	In the present study, the prebiotic potential of a FVBP flour obtained from solid byproducts after fruit and vegetable processing was evaluated after in vitro gastrointestinal digestion.
33233058	5	80	theme	real-time	904:912	arg1	qPCR					941:944	qPCR	941:944	qPCR	941:944	The changes in gut microbiota were evaluated at 0, 12, 24 and 48 h of fermentation by the real-time polymerase chain reaction (qPCR) method with 16S rRNA-based specific primers.
33233058	5	80	theme	real-time	904:912	arg1	reaction					931:938	the real-time polymerase chain reaction	900:938	the real-time polymerase chain reaction (qPCR) method with 16S rRNA-based specific primers	900:989	The changes in gut microbiota were evaluated at 0, 12, 24 and 48 h of fermentation by the real-time polymerase chain reaction (qPCR) method with 16S rRNA-based specific primers.
33233058	5	81	theme	chain	925:929	arg1	qPCR					941:944	qPCR	941:944	qPCR	941:944	The changes in gut microbiota were evaluated at 0, 12, 24 and 48 h of fermentation by the real-time polymerase chain reaction (qPCR) method with 16S rRNA-based specific primers.
33233058	5	81	theme	chain	925:929	arg1	reaction					931:938	the real-time polymerase chain reaction	900:938	the real-time polymerase chain reaction (qPCR) method with 16S rRNA-based specific primers	900:989	The changes in gut microbiota were evaluated at 0, 12, 24 and 48 h of fermentation by the real-time polymerase chain reaction (qPCR) method with 16S rRNA-based specific primers.
33233058	6	82	theme	fatty	1015:1019	arg1	SCFA					1028:1031	SCFA	1028:1031	SCFA	1028:1031	The pH and short chain fatty acids (SCFA) production at each fermentation time were assessed.
33233058	6	82	theme	fatty	1015:1019	arg1	acids					1021:1025	short chain fatty acids	1003:1025	short chain fatty acids (SCFA) production	1003:1043	The pH and short chain fatty acids (SCFA) production at each fermentation time were assessed.
33233058	10	83	theme	SCFA	1432:1435	arg1	production					1437:1446	SCFA production	1432:1446	SCFA production	1432:1446	SCFA production was observed when FVBP flour was used as carbon source, including butyrate, which supports the prebiotic potential of this flour.
33233058	11	84	theme	in	1619:1620	arg1	digestion					1645:1653	in vitro gastrointestinal digestion	1619:1653	in vitro gastrointestinal digestion	1619:1653	Additionally, it was observed that after in vitro gastrointestinal digestion, the FVBP flour at 3% promoted cell metabolism of Caco-2 cell line up to 67%.
33233058	4	85	theme	FVBP	754:757	arg1	flour					759:763	FVBP flour	754:763	FVBP flour	754:763	lactis BB12®) was carried out and then the prebiotic effect of FVBP flour was performed with fecal samples of five donors.
33233058	2	86	theme	vegetable	404:412	arg1	processing					414:423	fruit and vegetable processing	394:423	fruit and vegetable processing	394:423	In the present study, the prebiotic potential of a FVBP flour obtained from solid byproducts after fruit and vegetable processing was evaluated after in vitro gastrointestinal digestion.
33233058	9	87	theme	Lactobacillus	1291:1303	arg1	enhancement					1276:1286	growth enhancement	1269:1286	growth enhancement of Lactobacillus after 48 h of fermentation	1269:1330	FVBP flour showed higher prebiotic effect than FOS on growth enhancement of Lactobacillus after 48 h of fermentation and similar bifidogenic effect as FOS on Bifidobacterium growth at 12, 24 and 48 h of fermentation.
33233058	6	88	theme	short	1003:1007	arg1	SCFA					1028:1031	SCFA	1028:1031	SCFA	1028:1031	The pH and short chain fatty acids (SCFA) production at each fermentation time were assessed.
33233058	6	88	theme	short	1003:1007	arg1	acids					1021:1025	short chain fatty acids	1003:1025	short chain fatty acids (SCFA) production	1003:1043	The pH and short chain fatty acids (SCFA) production at each fermentation time were assessed.
33233058	11	89	dep	in	1619:1620	arg1	vitro					1622:1626	vitro	1622:1626	vitro	1622:1626	Additionally, it was observed that after in vitro gastrointestinal digestion, the FVBP flour at 3% promoted cell metabolism of Caco-2 cell line up to 67%.
33233058	2	90	theme	fruit	394:398	arg1	processing					414:423	fruit and vegetable processing	394:423	fruit and vegetable processing	394:423	In the present study, the prebiotic potential of a FVBP flour obtained from solid byproducts after fruit and vegetable processing was evaluated after in vitro gastrointestinal digestion.
33233058	0	91	theme	byproducts	50:59	arg1	flour					61:65	fruit and vegetable byproducts flour	30:65	fruit and vegetable byproducts flour using in vitro gastrointestinal digestion	30:107	Potential prebiotic effect of fruit and vegetable byproducts flour using in vitro gastrointestinal digestion.
33233058	9	92	theme	fermentation	1319:1330	arg1	48 h					1311:1314	48 h	1311:1314	48 h of fermentation	1311:1330	FVBP flour showed higher prebiotic effect than FOS on growth enhancement of Lactobacillus after 48 h of fermentation and similar bifidogenic effect as FOS on Bifidobacterium growth at 12, 24 and 48 h of fermentation.
33233058	11	93	theme	gastrointestinal	1628:1643	arg1	digestion					1645:1653	in vitro gastrointestinal digestion	1619:1653	in vitro gastrointestinal digestion	1619:1653	Additionally, it was observed that after in vitro gastrointestinal digestion, the FVBP flour at 3% promoted cell metabolism of Caco-2 cell line up to 67%.
33233058	2	94	theme	flour	351:355	arg1	potential					331:339	the prebiotic potential	317:339	the prebiotic potential of a FVBP flour obtained from solid byproducts after fruit and vegetable processing	317:423	In the present study, the prebiotic potential of a FVBP flour obtained from solid byproducts after fruit and vegetable processing was evaluated after in vitro gastrointestinal digestion.
33233058	9	95	theme	similar	1336:1342	arg1	effect					1356:1361	similar bifidogenic effect	1336:1361	similar bifidogenic effect as FOS on Bifidobacterium growth	1336:1394	FVBP flour showed higher prebiotic effect than FOS on growth enhancement of Lactobacillus after 48 h of fermentation and similar bifidogenic effect as FOS on Bifidobacterium growth at 12, 24 and 48 h of fermentation.
33233058	5	96	theme	rRNA-based	963:972	arg1	primers					983:989	16S rRNA-based specific primers	959:989	16S rRNA-based specific primers	959:989	The changes in gut microbiota were evaluated at 0, 12, 24 and 48 h of fermentation by the real-time polymerase chain reaction (qPCR) method with 16S rRNA-based specific primers.
33233058	6	97	theme	fermentation	1053:1064	arg1	time					1066:1069	each fermentation time	1048:1069	each fermentation time	1048:1069	The pH and short chain fatty acids (SCFA) production at each fermentation time were assessed.
33233058	12	98	theme	potential	1837:1845	arg1	flour					1826:1830	a fruit and vegetable byproducts flour	1793:1830	a fruit and vegetable byproducts flour	1793:1830	Thus, the present study demonstrates the viability of using a fruit and vegetable byproducts flour as a potential sustainable prebiotic source.
33233058	12	98	theme	potential	1837:1845	arg1	source					1869:1874	a potential sustainable prebiotic source	1835:1874	a potential sustainable prebiotic source	1835:1874	Thus, the present study demonstrates the viability of using a fruit and vegetable byproducts flour as a potential sustainable prebiotic source.
33233058	3	99	theme	Lactobacillus	525:537	arg1	strains					514:520	three strains	508:520	three strains of Lactobacillus (Lactobacillus casei 01, Lactobacillus rhamnosus R11 and Lactobacillus acidophilus LA-5®)	508:627	An initial screening with three strains of Lactobacillus (Lactobacillus casei 01, Lactobacillus rhamnosus R11 and Lactobacillus acidophilus LA-5®) and one Bifidobacterium strain (Bifidobacterium animalis spp.
33233058	3	99	theme	Lactobacillus	525:537	arg1	strain					653:658	one Bifidobacterium strain	633:658	one Bifidobacterium strain	633:658	An initial screening with three strains of Lactobacillus (Lactobacillus casei 01, Lactobacillus rhamnosus R11 and Lactobacillus acidophilus LA-5®) and one Bifidobacterium strain (Bifidobacterium animalis spp.
33233058	1	100	theme	positive	235:242	arg1	effect					255:260	a positive modulatory effect	233:260	a positive modulatory effect upon gut microbiota composition	233:292	Fruit and vegetable byproducts (FVBP) present high content of bioactive compounds and dietary fibers and have demonstrated a positive modulatory effect upon gut microbiota composition.
33115951	8	0	theme	sugar-fed	1098:1106	arg1	mice					1127:1130	sugar-fed wild-type and Il10 mice	1098:1130	sugar-fed wild-type and Il10 mice	1098:1130	Consistently, bacteria-derived mucolytic enzymes were enriched leading to erosion of the colonic mucus layer of sugar-fed wild-type and Il10 mice.
33115951	6	1	theme	gut	833:835	arg1	composition					848:858	gut microbiota composition	833:858	gut microbiota composition	833:858	Short-term intake of high glucose or fructose did not trigger inflammatory responses in healthy gut but markedly altered gut microbiota composition.
33115951	4	2	theme	Wild-type	480:488	arg1	mice					490:493	Wild-type mice	480:493	Wild-type mice fed 10% glucose in drinking water or high-glucose diet	480:548	Wild-type mice fed 10% glucose in drinking water or high-glucose diet developed severe colitis induced by dextran sulfate sodium.
33115951	6	3	theme	high	733:736	arg1	glucose					738:744	glucose	738:744	glucose	738:744	Short-term intake of high glucose or fructose did not trigger inflammatory responses in healthy gut but markedly altered gut microbiota composition.
33115951	8	4	link	bacteria-derived	1000:1015	arg1	enzymes					1027:1033	bacteria-derived mucolytic enzymes	1000:1033	bacteria-derived mucolytic enzymes	1000:1033	Consistently, bacteria-derived mucolytic enzymes were enriched leading to erosion of the colonic mucus layer of sugar-fed wild-type and Il10 mice.
33115951	2	5	theme	noncommunicable	255:269	arg1	diseases					271:278	many noncommunicable diseases	250:278	many noncommunicable diseases	250:278	High sugar, which is linked to many noncommunicable diseases, is a hallmark of the Western diet, but its role in IBD remains unknown.
33115951	8	6	theme	mice	1127:1130	arg1	layer					1089:1093	the colonic mucus layer	1071:1093	the colonic mucus layer of sugar-fed wild-type and Il10 mice	1071:1130	Consistently, bacteria-derived mucolytic enzymes were enriched leading to erosion of the colonic mucus layer of sugar-fed wild-type and Il10 mice.
33115951	9	7	theme	colitis	1163:1169	arg1	exacerbation					1147:1158	Sugar-induced exacerbation	1133:1158	Sugar-induced exacerbation of colitis	1133:1169	Sugar-induced exacerbation of colitis was not observed when mice were treated with antibiotics or maintained in a germ-free environment, suggesting that altered microbiota played a critical role in sugar-induced colitis pathogenesis.
33115951	3	8	theme	colitis	431:437	arg1	pathogenesis					439:450	colitis pathogenesis	431:450	colitis pathogenesis in wild-type and Il10 mice	431:477	Here, we studied the effects of simple sugars such as glucose and fructose on colitis pathogenesis in wild-type and Il10 mice.
33115951	2	9	theme	many	250:253	arg1	diseases					271:278	many noncommunicable diseases	250:278	many noncommunicable diseases	250:278	High sugar, which is linked to many noncommunicable diseases, is a hallmark of the Western diet, but its role in IBD remains unknown.
33115951	11	10	theme	sugars	1537:1542	arg1	intake					1520:1525	intake	1520:1525	intake of simple sugars	1520:1542	Together, these data suggest that intake of simple sugars predisposes to colitis and enhances its pathogenesis via modulation of gut microbiota in mice.
33115951	4	11	theme	high-glucose	532:543	arg1	diet					545:548	high-glucose diet	532:548	high-glucose diet	532:548	Wild-type mice fed 10% glucose in drinking water or high-glucose diet developed severe colitis induced by dextran sulfate sodium.
33115951	5	12	theme	Il10	627:630	arg1	mice					632:635	High-glucose-fed Il10 mice	610:635	High-glucose-fed Il10 mice	610:635	High-glucose-fed Il10 mice also developed a worsened colitis compared to glucose-untreated Il10 mice.
33115951	7	13	theme	Bacteroides	950:960	arg1	bacteria					913:920	the mucus-degrading bacteria	893:920	the mucus-degrading bacteria Akkermansia muciniphila and Bacteroides fragilis	893:969	In particular, the abundance of the mucus-degrading bacteria Akkermansia muciniphila and Bacteroides fragilis was increased.
33115951	11	14	from	modulation	1601:1610	arg1	mice					1633:1636	mice	1633:1636	mice	1633:1636	Together, these data suggest that intake of simple sugars predisposes to colitis and enhances its pathogenesis via modulation of gut microbiota in mice.
33115951	7	15	theme	bacteria	913:920	arg1	abundance					880:888	the abundance	876:888	the abundance of the mucus-degrading bacteria Akkermansia muciniphila and Bacteroides fragilis	876:969	In particular, the abundance of the mucus-degrading bacteria Akkermansia muciniphila and Bacteroides fragilis was increased.
33115951	1	16	theme	inflammatory	111:122	arg1	disease					130:136	inflammatory bowel disease	111:136	inflammatory bowel disease (IBD)	111:142	The higher prevalence of inflammatory bowel disease (IBD) in Western countries points to Western diet as a possible IBD risk factor.
33115951	1	16	theme	inflammatory	111:122	arg1	IBD					139:141	IBD	139:141	IBD	139:141	The higher prevalence of inflammatory bowel disease (IBD) in Western countries points to Western diet as a possible IBD risk factor.
33115951	4	17	from	glucose	503:509	arg1	water					523:527	drinking water	514:527	drinking water	514:527	Wild-type mice fed 10% glucose in drinking water or high-glucose diet developed severe colitis induced by dextran sulfate sodium.
33115951	4	17	from	glucose	503:509	arg1	diet					545:548	high-glucose diet	532:548	high-glucose diet	532:548	Wild-type mice fed 10% glucose in drinking water or high-glucose diet developed severe colitis induced by dextran sulfate sodium.
33115951	1	18	theme	disease	130:136	arg1	prevalence					97:106	The higher prevalence	86:106	The higher prevalence of inflammatory bowel disease (IBD) in Western countries	86:163	The higher prevalence of inflammatory bowel disease (IBD) in Western countries points to Western diet as a possible IBD risk factor.
33115951	9	19	theme	germ-free	1247:1255	arg1	environment					1257:1267	a germ-free environment	1245:1267	a germ-free environment	1245:1267	Sugar-induced exacerbation of colitis was not observed when mice were treated with antibiotics or maintained in a germ-free environment, suggesting that altered microbiota played a critical role in sugar-induced colitis pathogenesis.
33115951	10	20	theme	increased	1452:1460	arg1	susceptibility					1470:1483	increased colitis susceptibility	1452:1483	increased colitis susceptibility	1452:1483	Furthermore, germ-free mice colonized with microbiota from sugar-treated mice showed increased colitis susceptibility.
33115951	0	21	theme	Dietary	0:6	arg1	sugars					15:20	Dietary simple sugars	0:20	Dietary simple sugars	0:20	Dietary simple sugars alter microbial ecology in the gut and promote colitis in mice.
33115951	8	22	theme	mucolytic	1017:1025	arg1	enzymes					1027:1033	bacteria-derived mucolytic enzymes	1000:1033	bacteria-derived mucolytic enzymes	1000:1033	Consistently, bacteria-derived mucolytic enzymes were enriched leading to erosion of the colonic mucus layer of sugar-fed wild-type and Il10 mice.
33115951	6	23	theme	Short-term	712:721	arg1	intake					723:728	Short-term intake	712:728	Short-term intake of high glucose or fructose	712:756	Short-term intake of high glucose or fructose did not trigger inflammatory responses in healthy gut but markedly altered gut microbiota composition.
33115951	11	24	from	mice	1633:1636	arg1	modulation					1601:1610	modulation	1601:1610	modulation of gut microbiota in mice	1601:1636	Together, these data suggest that intake of simple sugars predisposes to colitis and enhances its pathogenesis via modulation of gut microbiota in mice.
33115951	7	25	dep	Akkermansia	922:932	arg1	muciniphila					934:944	muciniphila	934:944	muciniphila	934:944	In particular, the abundance of the mucus-degrading bacteria Akkermansia muciniphila and Bacteroides fragilis was increased.
33115951	0	26	theme	microbial	28:36	arg1	ecology					38:44	microbial ecology	28:44	microbial ecology in the gut	28:55	Dietary simple sugars alter microbial ecology in the gut and promote colitis in mice.
33115951	5	27	theme	Il10	701:704	arg1	mice					706:709	glucose-untreated Il10 mice	683:709	glucose-untreated Il10 mice	683:709	High-glucose-fed Il10 mice also developed a worsened colitis compared to glucose-untreated Il10 mice.
33115951	3	28	theme	sugars	392:397	arg1	effects					374:380	the effects	370:380	the effects of simple sugars such as glucose and fructose on colitis pathogenesis in wild-type and Il10 mice	370:477	Here, we studied the effects of simple sugars such as glucose and fructose on colitis pathogenesis in wild-type and Il10 mice.
33115951	9	29	theme	sugar-induced	1331:1343	arg1	pathogenesis					1353:1364	sugar-induced colitis pathogenesis	1331:1364	sugar-induced colitis pathogenesis	1331:1364	Sugar-induced exacerbation of colitis was not observed when mice were treated with antibiotics or maintained in a germ-free environment, suggesting that altered microbiota played a critical role in sugar-induced colitis pathogenesis.
33115951	3	30	from	effects	374:380	arg1	pathogenesis					439:450	colitis pathogenesis	431:450	colitis pathogenesis in wild-type and Il10 mice	431:477	Here, we studied the effects of simple sugars such as glucose and fructose on colitis pathogenesis in wild-type and Il10 mice.
33115951	4	31	theme	10	499:500	arg1	%					501:501	%	501:501	%	501:501	Wild-type mice fed 10% glucose in drinking water or high-glucose diet developed severe colitis induced by dextran sulfate sodium.
33115951	2	32	theme	Western	302:308	arg1	diet					310:313	the Western diet	298:313	the Western diet	298:313	High sugar, which is linked to many noncommunicable diseases, is a hallmark of the Western diet, but its role in IBD remains unknown.
33115951	1	33	theme	possible	193:200	arg1	factor					211:216	a possible IBD risk factor	191:216	a possible IBD risk factor	191:216	The higher prevalence of inflammatory bowel disease (IBD) in Western countries points to Western diet as a possible IBD risk factor.
33115951	8	34	theme	mucus	1083:1087	arg1	layer					1089:1093	the colonic mucus layer	1071:1093	the colonic mucus layer of sugar-fed wild-type and Il10 mice	1071:1130	Consistently, bacteria-derived mucolytic enzymes were enriched leading to erosion of the colonic mucus layer of sugar-fed wild-type and Il10 mice.
33115951	6	35	theme	microbiota	837:846	arg1	composition					848:858	gut microbiota composition	833:858	gut microbiota composition	833:858	Short-term intake of high glucose or fructose did not trigger inflammatory responses in healthy gut but markedly altered gut microbiota composition.
33115951	11	36	theme	gut	1615:1617	arg1	microbiota					1619:1628	gut microbiota	1615:1628	gut microbiota in mice	1615:1636	Together, these data suggest that intake of simple sugars predisposes to colitis and enhances its pathogenesis via modulation of gut microbiota in mice.
33115951	1	37	theme	risk	206:209	arg1	factor					211:216	a possible IBD risk factor	191:216	a possible IBD risk factor	191:216	The higher prevalence of inflammatory bowel disease (IBD) in Western countries points to Western diet as a possible IBD risk factor.
33115951	9	38	theme	Sugar-induced	1133:1145	arg1	exacerbation					1147:1158	Sugar-induced exacerbation	1133:1158	Sugar-induced exacerbation of colitis	1133:1169	Sugar-induced exacerbation of colitis was not observed when mice were treated with antibiotics or maintained in a germ-free environment, suggesting that altered microbiota played a critical role in sugar-induced colitis pathogenesis.
33115951	8	39	theme	wild-type	1108:1116	arg1	mice					1127:1130	sugar-fed wild-type and Il10 mice	1098:1130	sugar-fed wild-type and Il10 mice	1098:1130	Consistently, bacteria-derived mucolytic enzymes were enriched leading to erosion of the colonic mucus layer of sugar-fed wild-type and Il10 mice.
33115951	11	40	theme	simple	1530:1535	arg1	sugars					1537:1542	simple sugars	1530:1542	simple sugars	1530:1542	Together, these data suggest that intake of simple sugars predisposes to colitis and enhances its pathogenesis via modulation of gut microbiota in mice.
33115951	11	41	from	microbiota	1619:1628	arg1	mice					1633:1636	mice	1633:1636	mice	1633:1636	Together, these data suggest that intake of simple sugars predisposes to colitis and enhances its pathogenesis via modulation of gut microbiota in mice.
33115951	8	42	theme	Il10	1122:1125	arg1	mice					1127:1130	sugar-fed wild-type and Il10 mice	1098:1130	sugar-fed wild-type and Il10 mice	1098:1130	Consistently, bacteria-derived mucolytic enzymes were enriched leading to erosion of the colonic mucus layer of sugar-fed wild-type and Il10 mice.
33115951	6	43	theme	healthy	800:806	arg1	gut					808:810	healthy gut	800:810	healthy gut	800:810	Short-term intake of high glucose or fructose did not trigger inflammatory responses in healthy gut but markedly altered gut microbiota composition.
33115951	3	44	theme	Il10	469:472	arg1	mice					474:477	wild-type and Il10 mice	455:477	wild-type and Il10 mice	455:477	Here, we studied the effects of simple sugars such as glucose and fructose on colitis pathogenesis in wild-type and Il10 mice.
33115951	1	45	theme	Western	147:153	arg1	countries					155:163	Western countries	147:163	Western countries	147:163	The higher prevalence of inflammatory bowel disease (IBD) in Western countries points to Western diet as a possible IBD risk factor.
33115951	4	46	theme	severe	560:565	arg1	colitis					567:573	severe colitis	560:573	severe colitis induced by dextran sulfate sodium	560:607	Wild-type mice fed 10% glucose in drinking water or high-glucose diet developed severe colitis induced by dextran sulfate sodium.
33115951	6	47	from	responses	787:795	arg1	gut					808:810	healthy gut	800:810	healthy gut	800:810	Short-term intake of high glucose or fructose did not trigger inflammatory responses in healthy gut but markedly altered gut microbiota composition.
33115951	10	48	theme	germ-free	1380:1388	arg1	mice					1390:1393	germ-free mice	1380:1393	germ-free mice colonized with microbiota from sugar-treated mice	1380:1443	Furthermore, germ-free mice colonized with microbiota from sugar-treated mice showed increased colitis susceptibility.
33115951	3	49	theme	wild-type	455:463	arg1	mice					474:477	wild-type and Il10 mice	455:477	wild-type and Il10 mice	455:477	Here, we studied the effects of simple sugars such as glucose and fructose on colitis pathogenesis in wild-type and Il10 mice.
33115951	2	50	attach	linked	240:245	arg2	sugar					224:228	High sugar	219:228	High sugar	219:228	High sugar, which is linked to many noncommunicable diseases, is a hallmark of the Western diet, but its role in IBD remains unknown.
33115951	2	50	attach	linked	240:245	arg1	diseases					271:278	many noncommunicable diseases	250:278	many noncommunicable diseases	250:278	High sugar, which is linked to many noncommunicable diseases, is a hallmark of the Western diet, but its role in IBD remains unknown.
33115951	2	50	attach	linked	240:245	arg2	hallmark					286:293	a hallmark	284:293	a hallmark of the Western diet	284:313	High sugar, which is linked to many noncommunicable diseases, is a hallmark of the Western diet, but its role in IBD remains unknown.
33115951	0	51	from	colitis	69:75	arg1	mice					80:83	mice	80:83	mice	80:83	Dietary simple sugars alter microbial ecology in the gut and promote colitis in mice.
33115951	4	52	theme	drinking	514:521	arg1	water					523:527	drinking water	514:527	drinking water	514:527	Wild-type mice fed 10% glucose in drinking water or high-glucose diet developed severe colitis induced by dextran sulfate sodium.
33115951	5	53	theme	High-glucose-fed	610:625	arg1	mice					632:635	High-glucose-fed Il10 mice	610:635	High-glucose-fed Il10 mice	610:635	High-glucose-fed Il10 mice also developed a worsened colitis compared to glucose-untreated Il10 mice.
33115951	1	54	theme	Western	175:181	arg1	diet					183:186	Western diet	175:186	Western diet	175:186	The higher prevalence of inflammatory bowel disease (IBD) in Western countries points to Western diet as a possible IBD risk factor.
33115951	1	55	theme	higher	90:95	arg1	prevalence					97:106	The higher prevalence	86:106	The higher prevalence of inflammatory bowel disease (IBD) in Western countries	86:163	The higher prevalence of inflammatory bowel disease (IBD) in Western countries points to Western diet as a possible IBD risk factor.
33115951	2	56	theme	High	219:222	arg1	sugar					224:228	High sugar	219:228	High sugar	219:228	High sugar, which is linked to many noncommunicable diseases, is a hallmark of the Western diet, but its role in IBD remains unknown.
33115951	2	56	theme	High	219:222	arg1	hallmark					286:293	a hallmark	284:293	a hallmark of the Western diet	284:313	High sugar, which is linked to many noncommunicable diseases, is a hallmark of the Western diet, but its role in IBD remains unknown.
33115951	5	57	theme	worsened	654:661	arg1	colitis					663:669	a worsened colitis	652:669	a worsened colitis	652:669	High-glucose-fed Il10 mice also developed a worsened colitis compared to glucose-untreated Il10 mice.
33115951	7	58	theme	Akkermansia	922:932	arg1	bacteria					913:920	the mucus-degrading bacteria	893:920	the mucus-degrading bacteria Akkermansia muciniphila and Bacteroides fragilis	893:969	In particular, the abundance of the mucus-degrading bacteria Akkermansia muciniphila and Bacteroides fragilis was increased.
33115951	1	59	theme	bowel	124:128	arg1	disease					130:136	inflammatory bowel disease	111:136	inflammatory bowel disease (IBD)	111:142	The higher prevalence of inflammatory bowel disease (IBD) in Western countries points to Western diet as a possible IBD risk factor.
33115951	1	59	theme	bowel	124:128	arg1	IBD					139:141	IBD	139:141	IBD	139:141	The higher prevalence of inflammatory bowel disease (IBD) in Western countries points to Western diet as a possible IBD risk factor.
33115951	10	60	from	mice	1440:1443	arg1	microbiota					1410:1419	microbiota	1410:1419	microbiota from sugar-treated mice	1410:1443	Furthermore, germ-free mice colonized with microbiota from sugar-treated mice showed increased colitis susceptibility.
33115951	7	61	theme	mucus-degrading	897:911	arg1	bacteria					913:920	the mucus-degrading bacteria	893:920	the mucus-degrading bacteria Akkermansia muciniphila and Bacteroides fragilis	893:969	In particular, the abundance of the mucus-degrading bacteria Akkermansia muciniphila and Bacteroides fragilis was increased.
33115951	10	62	theme	sugar-treated	1426:1438	arg1	mice					1440:1443	sugar-treated mice	1426:1443	sugar-treated mice	1426:1443	Furthermore, germ-free mice colonized with microbiota from sugar-treated mice showed increased colitis susceptibility.
33115951	4	63	theme	dextran	586:592	arg1	sulfate					594:600	dextran sulfate sodium	586:607	dextran sulfate sodium	586:607	Wild-type mice fed 10% glucose in drinking water or high-glucose diet developed severe colitis induced by dextran sulfate sodium.
33115951	3	64	from	pathogenesis	439:450	arg1	mice					474:477	wild-type and Il10 mice	455:477	wild-type and Il10 mice	455:477	Here, we studied the effects of simple sugars such as glucose and fructose on colitis pathogenesis in wild-type and Il10 mice.
33115951	4	65	dep	sulfate	594:600	arg1	sodium					602:607	sodium	602:607	dextran sulfate sodium	586:607	Wild-type mice fed 10% glucose in drinking water or high-glucose diet developed severe colitis induced by dextran sulfate sodium.
33115951	8	66	theme	bacteria-derived	1000:1015	arg1	enzymes					1027:1033	bacteria-derived mucolytic enzymes	1000:1033	bacteria-derived mucolytic enzymes	1000:1033	Consistently, bacteria-derived mucolytic enzymes were enriched leading to erosion of the colonic mucus layer of sugar-fed wild-type and Il10 mice.
33115951	0	67	from	ecology	38:44	arg1	gut					53:55	the gut	49:55	the gut	49:55	Dietary simple sugars alter microbial ecology in the gut and promote colitis in mice.
33115951	10	68	theme	colitis	1462:1468	arg1	susceptibility					1470:1483	increased colitis susceptibility	1452:1483	increased colitis susceptibility	1452:1483	Furthermore, germ-free mice colonized with microbiota from sugar-treated mice showed increased colitis susceptibility.
33115951	7	69	dep	Bacteroides	950:960	arg1	fragilis					962:969	fragilis	962:969	fragilis	962:969	In particular, the abundance of the mucus-degrading bacteria Akkermansia muciniphila and Bacteroides fragilis was increased.
33115951	0	70	theme	simple	8:13	arg1	sugars					15:20	Dietary simple sugars	0:20	Dietary simple sugars	0:20	Dietary simple sugars alter microbial ecology in the gut and promote colitis in mice.
33115951	9	71	theme	altered	1286:1292	arg1	microbiota					1294:1303	altered microbiota	1286:1303	altered microbiota	1286:1303	Sugar-induced exacerbation of colitis was not observed when mice were treated with antibiotics or maintained in a germ-free environment, suggesting that altered microbiota played a critical role in sugar-induced colitis pathogenesis.
33115951	2	72	theme	diet	310:313	arg1	sugar					224:228	High sugar	219:228	High sugar	219:228	High sugar, which is linked to many noncommunicable diseases, is a hallmark of the Western diet, but its role in IBD remains unknown.
33115951	2	72	theme	diet	310:313	arg1	hallmark					286:293	a hallmark	284:293	a hallmark of the Western diet	284:313	High sugar, which is linked to many noncommunicable diseases, is a hallmark of the Western diet, but its role in IBD remains unknown.
33115951	1	73	from	prevalence	97:106	arg1	countries					155:163	Western countries	147:163	Western countries	147:163	The higher prevalence of inflammatory bowel disease (IBD) in Western countries points to Western diet as a possible IBD risk factor.
33115951	5	74	theme	glucose-untreated	683:699	arg1	mice					706:709	glucose-untreated Il10 mice	683:709	glucose-untreated Il10 mice	683:709	High-glucose-fed Il10 mice also developed a worsened colitis compared to glucose-untreated Il10 mice.
33115951	9	75	theme	critical	1314:1321	arg1	role					1323:1326	a critical role	1312:1326	a critical role	1312:1326	Sugar-induced exacerbation of colitis was not observed when mice were treated with antibiotics or maintained in a germ-free environment, suggesting that altered microbiota played a critical role in sugar-induced colitis pathogenesis.
33115951	6	76	theme	inflammatory	774:785	arg1	responses					787:795	inflammatory responses	774:795	inflammatory responses in healthy gut	774:810	Short-term intake of high glucose or fructose did not trigger inflammatory responses in healthy gut but markedly altered gut microbiota composition.
33115951	9	77	theme	colitis	1345:1351	arg1	pathogenesis					1353:1364	sugar-induced colitis pathogenesis	1331:1364	sugar-induced colitis pathogenesis	1331:1364	Sugar-induced exacerbation of colitis was not observed when mice were treated with antibiotics or maintained in a germ-free environment, suggesting that altered microbiota played a critical role in sugar-induced colitis pathogenesis.
33115951	8	78	theme	colonic	1075:1081	arg1	layer					1089:1093	the colonic mucus layer	1071:1093	the colonic mucus layer of sugar-fed wild-type and Il10 mice	1071:1130	Consistently, bacteria-derived mucolytic enzymes were enriched leading to erosion of the colonic mucus layer of sugar-fed wild-type and Il10 mice.
33115951	6	79	theme	fructose	749:756	arg1	intake					723:728	Short-term intake	712:728	Short-term intake of high glucose or fructose	712:756	Short-term intake of high glucose or fructose did not trigger inflammatory responses in healthy gut but markedly altered gut microbiota composition.
33115951	4	80	theme	%	501:501	arg1	glucose					503:509	10% glucose	499:509	10% glucose in drinking water or high-glucose diet	499:548	Wild-type mice fed 10% glucose in drinking water or high-glucose diet developed severe colitis induced by dextran sulfate sodium.
33115951	11	81	theme	microbiota	1619:1628	arg1	modulation					1601:1610	modulation	1601:1610	modulation of gut microbiota in mice	1601:1636	Together, these data suggest that intake of simple sugars predisposes to colitis and enhances its pathogenesis via modulation of gut microbiota in mice.
33115951	3	82	theme	simple	385:390	arg1	sugars					392:397	simple sugars	385:397	simple sugars such as glucose and fructose	385:426	Here, we studied the effects of simple sugars such as glucose and fructose on colitis pathogenesis in wild-type and Il10 mice.
33115951	3	82	theme	simple	385:390	arg1	glucose					407:413	glucose	407:413	glucose	407:413	Here, we studied the effects of simple sugars such as glucose and fructose on colitis pathogenesis in wild-type and Il10 mice.
33115951	3	82	theme	simple	385:390	arg1	fructose					419:426	fructose	419:426	fructose	419:426	Here, we studied the effects of simple sugars such as glucose and fructose on colitis pathogenesis in wild-type and Il10 mice.
33115951	8	83	theme	layer	1089:1093	arg1	erosion					1060:1066	erosion	1060:1066	erosion of the colonic mucus layer of sugar-fed wild-type and Il10 mice	1060:1130	Consistently, bacteria-derived mucolytic enzymes were enriched leading to erosion of the colonic mucus layer of sugar-fed wild-type and Il10 mice.
33115951	6	84	theme	glucose	738:744	arg1	intake					723:728	Short-term intake	712:728	Short-term intake of high glucose or fructose	712:756	Short-term intake of high glucose or fructose did not trigger inflammatory responses in healthy gut but markedly altered gut microbiota composition.
33115951	2	85	from	role	324:327	arg1	IBD					332:334	IBD	332:334	IBD	332:334	High sugar, which is linked to many noncommunicable diseases, is a hallmark of the Western diet, but its role in IBD remains unknown.
33115951	1	86	theme	IBD	202:204	arg1	factor					211:216	a possible IBD risk factor	191:216	a possible IBD risk factor	191:216	The higher prevalence of inflammatory bowel disease (IBD) in Western countries points to Western diet as a possible IBD risk factor.
32416195	2	0	theme	ram	377:379	arg1	sperm					381:385	ram sperm	377:385	ram sperm	377:385	We evaluated the extent of cholesterol and desmosterol incorporation into ram sperm through incubation with increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes, and its effect on membrane biophysical properties, membrane lateral organization and cryopreservation outcome.
32416195	6	1	theme	sterol	1326:1331	arg1	incorporation					1333:1345	sterol incorporation	1326:1345	sterol incorporation	1326:1345	Lateral organization of the plasma membrane, assessed by identification of GM1-related rafts, was preserved after sterol incorporation except when high levels of sterols (25 mM MβCD-desmosterol) were incorporated.
32416195	1	2	theme	insemination	280:291	arg1	programs					293:300	artificial insemination programs	269:300	artificial insemination programs	269:300	Ram sperm are particularly sensitive to freeze-thawing mainly due to their lipid composition, limiting their use in artificial insemination programs.
32416195	9	3	theme	biophysical	1884:1894	arg1	behavior					1896:1903	biophysical behavior	1884:1903	biophysical behavior	1884:1903	Our research provides evidence on the effective incorporation and biophysical behavior of cholesterol and desmosterol in ram sperm membranes and on their consequences in improving functional parameters of sperm after temperature decrease and freezing.
32416195	5	4	theme	cholesterol	982:992	arg1	imaging					959:965	Live cell imaging	949:965	Live cell imaging of fluorescent cholesterol	949:992	Live cell imaging of fluorescent cholesterol showed sterol incorporation and evidenced the presence of sperm sub-populations compatible with different sterol contents and a high concentration of sterol rich-ordered domains mainly at the acrosome plasma membrane.
32416195	6	5	theme	GM1-related	1287:1297	arg1	rafts					1299:1303	GM1-related rafts	1287:1303	GM1-related rafts	1287:1303	Lateral organization of the plasma membrane, assessed by identification of GM1-related rafts, was preserved after sterol incorporation except when high levels of sterols (25 mM MβCD-desmosterol) were incorporated.
32416195	5	6	theme	cell	954:957	arg1	imaging					959:965	Live cell imaging	949:965	Live cell imaging of fluorescent cholesterol	949:992	Live cell imaging of fluorescent cholesterol showed sterol incorporation and evidenced the presence of sperm sub-populations compatible with different sterol contents and a high concentration of sterol rich-ordered domains mainly at the acrosome plasma membrane.
32416195	8	7	theme	sperm	1644:1648	arg1	motility					1650:1657	sperm motility	1644:1657	sperm motility	1644:1657	While treatment with 10 mM MβCD-cholesterol increased sperm motility, membrane integrity and tolerance to osmotic stress after thawing, incorporation of desmosterol increased the ability of ram sperm to overcome osmotic stress.
32416195	7	8	theme	sperm	1537:1541	arg1	parameters					1551:1560	sperm quality parameters	1537:1560	sperm quality parameters	1537:1560	Ram sperm incubation with 10 mM MβCD-sterols prior to cryopreservation in a cholesterol-free extender improved sperm quality parameters after cooling and freezing.
32416195	4	9	theme	Differential	794:805	arg1	effect					816:821	Differential ordering effect	794:821	Differential ordering effect of sterols in ternary-mixture model membranes	794:867	Differential ordering effect of sterols in ternary-mixture model membranes revealed a reduced tendency of desmosterol of segregating into ordered domains.
32416195	8	10	with	treatment	1596:1604	arg1	MβCD-cholesterol					1617:1632	10 mM MβCD-cholesterol	1611:1632	10 mM MβCD-cholesterol	1611:1632	While treatment with 10 mM MβCD-cholesterol increased sperm motility, membrane integrity and tolerance to osmotic stress after thawing, incorporation of desmosterol increased the ability of ram sperm to overcome osmotic stress.
32416195	9	11	theme	sperm	1943:1947	arg1	membranes					1949:1957	ram sperm membranes	1939:1957	ram sperm membranes	1939:1957	Our research provides evidence on the effective incorporation and biophysical behavior of cholesterol and desmosterol in ram sperm membranes and on their consequences in improving functional parameters of sperm after temperature decrease and freezing.
32416195	6	12	theme	high	1359:1362	arg1	levels					1364:1369	high levels	1359:1369	high levels of sterols (25 mM MβCD-desmosterol)	1359:1405	Lateral organization of the plasma membrane, assessed by identification of GM1-related rafts, was preserved after sterol incorporation except when high levels of sterols (25 mM MβCD-desmosterol) were incorporated.
32416195	5	13	theme	acrosome	1186:1193	arg1	membrane					1202:1209	the acrosome plasma membrane	1182:1209	the acrosome plasma membrane	1182:1209	Live cell imaging of fluorescent cholesterol showed sterol incorporation and evidenced the presence of sperm sub-populations compatible with different sterol contents and a high concentration of sterol rich-ordered domains mainly at the acrosome plasma membrane.
32416195	2	14	theme	membrane	538:545	arg1	concentrations					422:435	increasing concentrations	411:435	increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes	411:484	We evaluated the extent of cholesterol and desmosterol incorporation into ram sperm through incubation with increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes, and its effect on membrane biophysical properties, membrane lateral organization and cryopreservation outcome.
32416195	2	14	theme	membrane	538:545	arg1	organization					555:566	membrane lateral organization	538:566	membrane lateral organization	538:566	We evaluated the extent of cholesterol and desmosterol incorporation into ram sperm through incubation with increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes, and its effect on membrane biophysical properties, membrane lateral organization and cryopreservation outcome.
32416195	9	15	theme	temperature	2035:2045	arg1	decrease					2047:2054	temperature decrease	2035:2054	temperature decrease	2035:2054	Our research provides evidence on the effective incorporation and biophysical behavior of cholesterol and desmosterol in ram sperm membranes and on their consequences in improving functional parameters of sperm after temperature decrease and freezing.
32416195	5	16	theme	sterol	1001:1006	arg1	incorporation					1008:1020	sterol incorporation	1001:1020	sterol incorporation	1001:1020	Live cell imaging of fluorescent cholesterol showed sterol incorporation and evidenced the presence of sperm sub-populations compatible with different sterol contents and a high concentration of sterol rich-ordered domains mainly at the acrosome plasma membrane.
32416195	2	17	theme	cryopreservation	572:587	arg1	concentrations					422:435	increasing concentrations	411:435	increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes	411:484	We evaluated the extent of cholesterol and desmosterol incorporation into ram sperm through incubation with increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes, and its effect on membrane biophysical properties, membrane lateral organization and cryopreservation outcome.
32416195	2	17	theme	cryopreservation	572:587	arg1	outcome					589:595	cryopreservation outcome	572:595	cryopreservation outcome	572:595	We evaluated the extent of cholesterol and desmosterol incorporation into ram sperm through incubation with increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes, and its effect on membrane biophysical properties, membrane lateral organization and cryopreservation outcome.
32416195	8	18	theme	osmotic	1696:1702	arg1	stress					1704:1709	osmotic stress	1696:1709	osmotic stress	1696:1709	While treatment with 10 mM MβCD-cholesterol increased sperm motility, membrane integrity and tolerance to osmotic stress after thawing, incorporation of desmosterol increased the ability of ram sperm to overcome osmotic stress.
32416195	5	19	theme	sterol	1100:1105	arg1	contents					1107:1114	different sterol contents	1090:1114	different sterol contents	1090:1114	Live cell imaging of fluorescent cholesterol showed sterol incorporation and evidenced the presence of sperm sub-populations compatible with different sterol contents and a high concentration of sterol rich-ordered domains mainly at the acrosome plasma membrane.
32416195	7	20	with	incubation	1436:1445	arg1	MβCD-sterols					1458:1469	10 mM MβCD-sterols	1452:1469	10 mM MβCD-sterols prior to cryopreservation	1452:1495	Ram sperm incubation with 10 mM MβCD-sterols prior to cryopreservation in a cholesterol-free extender improved sperm quality parameters after cooling and freezing.
32416195	9	21	theme	functional	1998:2007	arg1	parameters					2009:2018	functional parameters	1998:2018	functional parameters of sperm	1998:2027	Our research provides evidence on the effective incorporation and biophysical behavior of cholesterol and desmosterol in ram sperm membranes and on their consequences in improving functional parameters of sperm after temperature decrease and freezing.
32416195	0	22	theme	biophysical	111:121	arg1	properties					123:132	membrane biophysical properties	102:132	membrane biophysical properties	102:132	Cholesterol and desmosterol incorporation into ram sperm membrane before cryopreservation: Effects on membrane biophysical properties and sperm quality.
32416195	6	23	theme	Lateral	1212:1218	arg1	organization					1220:1231	Lateral organization	1212:1231	Lateral organization	1212:1231	Lateral organization of the plasma membrane, assessed by identification of GM1-related rafts, was preserved after sterol incorporation except when high levels of sterols (25 mM MβCD-desmosterol) were incorporated.
32416195	4	24	theme	sterols	826:832	arg1	effect					816:821	Differential ordering effect	794:821	Differential ordering effect of sterols in ternary-mixture model membranes	794:867	Differential ordering effect of sterols in ternary-mixture model membranes revealed a reduced tendency of desmosterol of segregating into ordered domains.
32416195	2	25	theme	increasing	411:420	arg1	concentrations					422:435	increasing concentrations	411:435	increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes	411:484	We evaluated the extent of cholesterol and desmosterol incorporation into ram sperm through incubation with increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes, and its effect on membrane biophysical properties, membrane lateral organization and cryopreservation outcome.
32416195	2	25	theme	increasing	411:420	arg1	organization					555:566	membrane lateral organization	538:566	membrane lateral organization	538:566	We evaluated the extent of cholesterol and desmosterol incorporation into ram sperm through incubation with increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes, and its effect on membrane biophysical properties, membrane lateral organization and cryopreservation outcome.
32416195	2	25	theme	increasing	411:420	arg1	outcome					589:595	cryopreservation outcome	572:595	cryopreservation outcome	572:595	We evaluated the extent of cholesterol and desmosterol incorporation into ram sperm through incubation with increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes, and its effect on membrane biophysical properties, membrane lateral organization and cryopreservation outcome.
32416195	5	26	theme	sub-populations	1058:1072	arg1	concentration					1127:1139	a high concentration	1120:1139	a high concentration of sterol rich-ordered domains	1120:1170	Live cell imaging of fluorescent cholesterol showed sterol incorporation and evidenced the presence of sperm sub-populations compatible with different sterol contents and a high concentration of sterol rich-ordered domains mainly at the acrosome plasma membrane.
32416195	5	26	theme	sub-populations	1058:1072	arg1	presence					1040:1047	the presence	1036:1047	the presence of sperm sub-populations compatible with different sterol contents	1036:1114	Live cell imaging of fluorescent cholesterol showed sterol incorporation and evidenced the presence of sperm sub-populations compatible with different sterol contents and a high concentration of sterol rich-ordered domains mainly at the acrosome plasma membrane.
32416195	2	27	theme	complexes	476:484	arg1	concentrations					422:435	increasing concentrations	411:435	increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes	411:484	We evaluated the extent of cholesterol and desmosterol incorporation into ram sperm through incubation with increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes, and its effect on membrane biophysical properties, membrane lateral organization and cryopreservation outcome.
32416195	2	27	theme	complexes	476:484	arg1	effect					495:500	its effect	491:500	its effect on membrane biophysical properties	491:535	We evaluated the extent of cholesterol and desmosterol incorporation into ram sperm through incubation with increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes, and its effect on membrane biophysical properties, membrane lateral organization and cryopreservation outcome.
32416195	2	27	theme	complexes	476:484	arg1	outcome					589:595	cryopreservation outcome	572:595	cryopreservation outcome	572:595	We evaluated the extent of cholesterol and desmosterol incorporation into ram sperm through incubation with increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes, and its effect on membrane biophysical properties, membrane lateral organization and cryopreservation outcome.
32416195	2	27	theme	complexes	476:484	arg1	organization					555:566	membrane lateral organization	538:566	membrane lateral organization	538:566	We evaluated the extent of cholesterol and desmosterol incorporation into ram sperm through incubation with increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes, and its effect on membrane biophysical properties, membrane lateral organization and cryopreservation outcome.
32416195	4	28	theme	ternary-mixture	837:851	arg1	membranes					859:867	ternary-mixture model membranes	837:867	ternary-mixture model membranes	837:867	Differential ordering effect of sterols in ternary-mixture model membranes revealed a reduced tendency of desmosterol of segregating into ordered domains.
32416195	0	29	theme	desmosterol	16:26	arg1	incorporation					28:40	desmosterol incorporation	16:40	desmosterol incorporation	16:40	Cholesterol and desmosterol incorporation into ram sperm membrane before cryopreservation: Effects on membrane biophysical properties and sperm quality.
32416195	2	30	theme	membrane	505:512	arg1	properties					526:535	membrane biophysical properties	505:535	membrane biophysical properties	505:535	We evaluated the extent of cholesterol and desmosterol incorporation into ram sperm through incubation with increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes, and its effect on membrane biophysical properties, membrane lateral organization and cryopreservation outcome.
32416195	3	31	theme	lipid	720:724	arg1	order					726:730	membrane lipid order	711:730	membrane lipid order	711:730	Sterols were effectively incorporated into the sperm membrane at 10 and 25 mM MβCD-sterols, similarly increasing membrane lipid order at physiological temperature and during temperature decrease.
32416195	5	32	theme	rich-ordered	1151:1162	arg1	domains					1164:1170	sterol rich-ordered domains	1144:1170	sterol rich-ordered domains	1144:1170	Live cell imaging of fluorescent cholesterol showed sterol incorporation and evidenced the presence of sperm sub-populations compatible with different sterol contents and a high concentration of sterol rich-ordered domains mainly at the acrosome plasma membrane.
32416195	7	33	from	incubation	1436:1445	arg1	extender					1519:1526	a cholesterol-free extender	1500:1526	a cholesterol-free extender	1500:1526	Ram sperm incubation with 10 mM MβCD-sterols prior to cryopreservation in a cholesterol-free extender improved sperm quality parameters after cooling and freezing.
32416195	4	34	theme	ordered	932:938	arg1	domains					940:946	ordered domains	932:946	ordered domains	932:946	Differential ordering effect of sterols in ternary-mixture model membranes revealed a reduced tendency of desmosterol of segregating into ordered domains.
32416195	0	35	theme	sperm	51:55	arg1	membrane					57:64	ram sperm membrane	47:64	ram sperm membrane before cryopreservation	47:88	Cholesterol and desmosterol incorporation into ram sperm membrane before cryopreservation: Effects on membrane biophysical properties and sperm quality.
32416195	7	36	theme	sperm	1430:1434	arg1	incubation					1436:1445	Ram sperm incubation	1426:1445	Ram sperm incubation with 10 mM MβCD-sterols prior to cryopreservation in a cholesterol-free extender	1426:1526	Ram sperm incubation with 10 mM MβCD-sterols prior to cryopreservation in a cholesterol-free extender improved sperm quality parameters after cooling and freezing.
32416195	8	37	theme	ram	1780:1782	arg1	sperm					1784:1788	ram sperm	1780:1788	ram sperm	1780:1788	While treatment with 10 mM MβCD-cholesterol increased sperm motility, membrane integrity and tolerance to osmotic stress after thawing, incorporation of desmosterol increased the ability of ram sperm to overcome osmotic stress.
32416195	2	38	theme	desmosterol	346:356	arg1	incorporation					358:370	desmosterol incorporation	346:370	desmosterol incorporation into ram sperm	346:385	We evaluated the extent of cholesterol and desmosterol incorporation into ram sperm through incubation with increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes, and its effect on membrane biophysical properties, membrane lateral organization and cryopreservation outcome.
32416195	2	39	with	incubation	395:404	arg1	concentrations					422:435	increasing concentrations	411:435	increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes	411:484	We evaluated the extent of cholesterol and desmosterol incorporation into ram sperm through incubation with increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes, and its effect on membrane biophysical properties, membrane lateral organization and cryopreservation outcome.
32416195	2	39	with	incubation	395:404	arg1	effect					495:500	its effect	491:500	its effect on membrane biophysical properties	491:535	We evaluated the extent of cholesterol and desmosterol incorporation into ram sperm through incubation with increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes, and its effect on membrane biophysical properties, membrane lateral organization and cryopreservation outcome.
32416195	2	39	with	incubation	395:404	arg1	outcome					589:595	cryopreservation outcome	572:595	cryopreservation outcome	572:595	We evaluated the extent of cholesterol and desmosterol incorporation into ram sperm through incubation with increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes, and its effect on membrane biophysical properties, membrane lateral organization and cryopreservation outcome.
32416195	2	39	with	incubation	395:404	arg1	organization					555:566	membrane lateral organization	538:566	membrane lateral organization	538:566	We evaluated the extent of cholesterol and desmosterol incorporation into ram sperm through incubation with increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes, and its effect on membrane biophysical properties, membrane lateral organization and cryopreservation outcome.
32416195	5	40	theme	high	1122:1125	arg1	concentration					1127:1139	a high concentration	1120:1139	a high concentration of sterol rich-ordered domains	1120:1170	Live cell imaging of fluorescent cholesterol showed sterol incorporation and evidenced the presence of sperm sub-populations compatible with different sterol contents and a high concentration of sterol rich-ordered domains mainly at the acrosome plasma membrane.
32416195	8	41	theme	10 mM	1611:1615	arg1	MβCD-cholesterol					1617:1632	10 mM MβCD-cholesterol	1611:1632	10 mM MβCD-cholesterol	1611:1632	While treatment with 10 mM MβCD-cholesterol increased sperm motility, membrane integrity and tolerance to osmotic stress after thawing, incorporation of desmosterol increased the ability of ram sperm to overcome osmotic stress.
32416195	0	42	dep	Cholesterol	0:10	arg1	Effects					91:97	Effects	91:97	Cholesterol and desmosterol incorporation into ram sperm membrane before cryopreservation: Effects on membrane biophysical properties and sperm quality.	0:151	Cholesterol and desmosterol incorporation into ram sperm membrane before cryopreservation: Effects on membrane biophysical properties and sperm quality.
32416195	6	43	theme	plasma	1240:1245	arg1	membrane					1247:1254	the plasma membrane	1236:1254	the plasma membrane	1236:1254	Lateral organization of the plasma membrane, assessed by identification of GM1-related rafts, was preserved after sterol incorporation except when high levels of sterols (25 mM MβCD-desmosterol) were incorporated.
32416195	8	44	theme	osmotic	1802:1808	arg1	stress					1810:1815	osmotic stress	1802:1815	osmotic stress	1802:1815	While treatment with 10 mM MβCD-cholesterol increased sperm motility, membrane integrity and tolerance to osmotic stress after thawing, incorporation of desmosterol increased the ability of ram sperm to overcome osmotic stress.
32416195	1	45	theme	artificial	269:278	arg1	programs					293:300	artificial insemination programs	269:300	artificial insemination programs	269:300	Ram sperm are particularly sensitive to freeze-thawing mainly due to their lipid composition, limiting their use in artificial insemination programs.
32416195	9	46	theme	effective	1856:1864	arg1	incorporation					1866:1878	effective incorporation	1856:1878	effective incorporation	1856:1878	Our research provides evidence on the effective incorporation and biophysical behavior of cholesterol and desmosterol in ram sperm membranes and on their consequences in improving functional parameters of sperm after temperature decrease and freezing.
32416195	5	47	with	compatible	1074:1083	arg1	contents					1107:1114	different sterol contents	1090:1114	different sterol contents	1090:1114	Live cell imaging of fluorescent cholesterol showed sterol incorporation and evidenced the presence of sperm sub-populations compatible with different sterol contents and a high concentration of sterol rich-ordered domains mainly at the acrosome plasma membrane.
32416195	2	48	theme	cholesterol	330:340	arg1	incorporation					358:370	desmosterol incorporation	346:370	desmosterol incorporation into ram sperm	346:385	We evaluated the extent of cholesterol and desmosterol incorporation into ram sperm through incubation with increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes, and its effect on membrane biophysical properties, membrane lateral organization and cryopreservation outcome.
32416195	2	48	theme	cholesterol	330:340	arg1	extent					320:325	the extent	316:325	the extent of cholesterol	316:340	We evaluated the extent of cholesterol and desmosterol incorporation into ram sperm through incubation with increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes, and its effect on membrane biophysical properties, membrane lateral organization and cryopreservation outcome.
32416195	5	49	theme	fluorescent	970:980	arg1	cholesterol					982:992	fluorescent cholesterol	970:992	fluorescent cholesterol	970:992	Live cell imaging of fluorescent cholesterol showed sterol incorporation and evidenced the presence of sperm sub-populations compatible with different sterol contents and a high concentration of sterol rich-ordered domains mainly at the acrosome plasma membrane.
32416195	6	50	theme	rafts	1299:1303	arg1	identification					1269:1282	identification	1269:1282	identification of GM1-related rafts	1269:1303	Lateral organization of the plasma membrane, assessed by identification of GM1-related rafts, was preserved after sterol incorporation except when high levels of sterols (25 mM MβCD-desmosterol) were incorporated.
32416195	0	51	theme	sperm	138:142	arg1	quality					144:150	sperm quality	138:150	sperm quality	138:150	Cholesterol and desmosterol incorporation into ram sperm membrane before cryopreservation: Effects on membrane biophysical properties and sperm quality.
32416195	9	52	from	incorporation	1866:1878	arg1	membranes					1949:1957	ram sperm membranes	1939:1957	ram sperm membranes	1939:1957	Our research provides evidence on the effective incorporation and biophysical behavior of cholesterol and desmosterol in ram sperm membranes and on their consequences in improving functional parameters of sperm after temperature decrease and freezing.
32416195	9	53	theme	cholesterol	1908:1918	arg1	incorporation					1866:1878	effective incorporation	1856:1878	effective incorporation	1856:1878	Our research provides evidence on the effective incorporation and biophysical behavior of cholesterol and desmosterol in ram sperm membranes and on their consequences in improving functional parameters of sperm after temperature decrease and freezing.
32416195	0	54	from	Effects	91:97	arg1	quality					144:150	sperm quality	138:150	sperm quality	138:150	Cholesterol and desmosterol incorporation into ram sperm membrane before cryopreservation: Effects on membrane biophysical properties and sperm quality.
32416195	0	54	from	Effects	91:97	arg1	properties					123:132	membrane biophysical properties	102:132	membrane biophysical properties	102:132	Cholesterol and desmosterol incorporation into ram sperm membrane before cryopreservation: Effects on membrane biophysical properties and sperm quality.
32416195	1	55	from	use	262:264	arg1	programs					293:300	artificial insemination programs	269:300	artificial insemination programs	269:300	Ram sperm are particularly sensitive to freeze-thawing mainly due to their lipid composition, limiting their use in artificial insemination programs.
32416195	5	56	theme	Live	949:952	arg1	imaging					959:965	Live cell imaging	949:965	Live cell imaging of fluorescent cholesterol	949:992	Live cell imaging of fluorescent cholesterol showed sterol incorporation and evidenced the presence of sperm sub-populations compatible with different sterol contents and a high concentration of sterol rich-ordered domains mainly at the acrosome plasma membrane.
32416195	9	57	theme	desmosterol	1924:1934	arg1	incorporation					1866:1878	effective incorporation	1856:1878	effective incorporation	1856:1878	Our research provides evidence on the effective incorporation and biophysical behavior of cholesterol and desmosterol in ram sperm membranes and on their consequences in improving functional parameters of sperm after temperature decrease and freezing.
32416195	6	58	theme	sterols	1374:1380	arg1	levels					1364:1369	high levels	1359:1369	high levels of sterols (25 mM MβCD-desmosterol)	1359:1405	Lateral organization of the plasma membrane, assessed by identification of GM1-related rafts, was preserved after sterol incorporation except when high levels of sterols (25 mM MβCD-desmosterol) were incorporated.
32416195	3	59	theme	sperm	645:649	arg1	membrane					651:658	the sperm membrane	641:658	the sperm membrane	641:658	Sterols were effectively incorporated into the sperm membrane at 10 and 25 mM MβCD-sterols, similarly increasing membrane lipid order at physiological temperature and during temperature decrease.
32416195	9	60	from	evidence	1840:1847	arg1	consequences					1972:1983	their consequences	1966:1983	their consequences in improving functional parameters of sperm after temperature decrease and freezing	1966:2067	Our research provides evidence on the effective incorporation and biophysical behavior of cholesterol and desmosterol in ram sperm membranes and on their consequences in improving functional parameters of sperm after temperature decrease and freezing.
32416195	9	60	from	evidence	1840:1847	arg1	incorporation					1866:1878	effective incorporation	1856:1878	effective incorporation	1856:1878	Our research provides evidence on the effective incorporation and biophysical behavior of cholesterol and desmosterol in ram sperm membranes and on their consequences in improving functional parameters of sperm after temperature decrease and freezing.
32416195	9	61	theme	ram	1939:1941	arg1	membranes					1949:1957	ram sperm membranes	1939:1957	ram sperm membranes	1939:1957	Our research provides evidence on the effective incorporation and biophysical behavior of cholesterol and desmosterol in ram sperm membranes and on their consequences in improving functional parameters of sperm after temperature decrease and freezing.
32416195	8	62	theme	membrane	1660:1667	arg1	integrity					1669:1677	membrane integrity	1660:1677	membrane integrity	1660:1677	While treatment with 10 mM MβCD-cholesterol increased sperm motility, membrane integrity and tolerance to osmotic stress after thawing, incorporation of desmosterol increased the ability of ram sperm to overcome osmotic stress.
32416195	7	63	theme	quality	1543:1549	arg1	parameters					1551:1560	sperm quality parameters	1537:1560	sperm quality parameters	1537:1560	Ram sperm incubation with 10 mM MβCD-sterols prior to cryopreservation in a cholesterol-free extender improved sperm quality parameters after cooling and freezing.
32416195	4	64	theme	ordering	807:814	arg1	effect					816:821	Differential ordering effect	794:821	Differential ordering effect of sterols in ternary-mixture model membranes	794:867	Differential ordering effect of sterols in ternary-mixture model membranes revealed a reduced tendency of desmosterol of segregating into ordered domains.
32416195	5	65	theme	plasma	1195:1200	arg1	membrane					1202:1209	the acrosome plasma membrane	1182:1209	the acrosome plasma membrane	1182:1209	Live cell imaging of fluorescent cholesterol showed sterol incorporation and evidenced the presence of sperm sub-populations compatible with different sterol contents and a high concentration of sterol rich-ordered domains mainly at the acrosome plasma membrane.
32416195	1	66	theme	Ram	153:155	arg1	sperm					157:161	Ram sperm	153:161	Ram sperm	153:161	Ram sperm are particularly sensitive to freeze-thawing mainly due to their lipid composition, limiting their use in artificial insemination programs.
32416195	7	67	theme	cholesterol-free	1502:1517	arg1	extender					1519:1526	a cholesterol-free extender	1500:1526	a cholesterol-free extender	1500:1526	Ram sperm incubation with 10 mM MβCD-sterols prior to cryopreservation in a cholesterol-free extender improved sperm quality parameters after cooling and freezing.
32416195	2	68	theme	biophysical	514:524	arg1	properties					526:535	membrane biophysical properties	505:535	membrane biophysical properties	505:535	We evaluated the extent of cholesterol and desmosterol incorporation into ram sperm through incubation with increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes, and its effect on membrane biophysical properties, membrane lateral organization and cryopreservation outcome.
32416195	0	69	theme	membrane	102:109	arg1	properties					123:132	membrane biophysical properties	102:132	membrane biophysical properties	102:132	Cholesterol and desmosterol incorporation into ram sperm membrane before cryopreservation: Effects on membrane biophysical properties and sperm quality.
32416195	5	70	theme	different	1090:1098	arg1	contents					1107:1114	different sterol contents	1090:1114	different sterol contents	1090:1114	Live cell imaging of fluorescent cholesterol showed sterol incorporation and evidenced the presence of sperm sub-populations compatible with different sterol contents and a high concentration of sterol rich-ordered domains mainly at the acrosome plasma membrane.
32416195	2	71	from	effect	495:500	arg1	properties					526:535	membrane biophysical properties	505:535	membrane biophysical properties	505:535	We evaluated the extent of cholesterol and desmosterol incorporation into ram sperm through incubation with increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes, and its effect on membrane biophysical properties, membrane lateral organization and cryopreservation outcome.
32416195	4	72	theme	model	853:857	arg1	membranes					859:867	ternary-mixture model membranes	837:867	ternary-mixture model membranes	837:867	Differential ordering effect of sterols in ternary-mixture model membranes revealed a reduced tendency of desmosterol of segregating into ordered domains.
32416195	2	73	theme	lateral	547:553	arg1	concentrations					422:435	increasing concentrations	411:435	increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes	411:484	We evaluated the extent of cholesterol and desmosterol incorporation into ram sperm through incubation with increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes, and its effect on membrane biophysical properties, membrane lateral organization and cryopreservation outcome.
32416195	2	73	theme	lateral	547:553	arg1	organization					555:566	membrane lateral organization	538:566	membrane lateral organization	538:566	We evaluated the extent of cholesterol and desmosterol incorporation into ram sperm through incubation with increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes, and its effect on membrane biophysical properties, membrane lateral organization and cryopreservation outcome.
32416195	5	74	theme	sperm	1052:1056	arg1	sub-populations					1058:1072	sperm sub-populations	1052:1072	sperm sub-populations compatible with different sterol contents	1052:1114	Live cell imaging of fluorescent cholesterol showed sterol incorporation and evidenced the presence of sperm sub-populations compatible with different sterol contents and a high concentration of sterol rich-ordered domains mainly at the acrosome plasma membrane.
32416195	9	75	theme	sperm	2023:2027	arg1	parameters					2009:2018	functional parameters	1998:2018	functional parameters of sperm	1998:2027	Our research provides evidence on the effective incorporation and biophysical behavior of cholesterol and desmosterol in ram sperm membranes and on their consequences in improving functional parameters of sperm after temperature decrease and freezing.
32416195	8	76	theme	desmosterol	1743:1753	arg1	incorporation					1726:1738	incorporation	1726:1738	incorporation of desmosterol	1726:1753	While treatment with 10 mM MβCD-cholesterol increased sperm motility, membrane integrity and tolerance to osmotic stress after thawing, incorporation of desmosterol increased the ability of ram sperm to overcome osmotic stress.
32416195	4	77	theme	desmosterol	900:910	arg1	tendency					888:895	a reduced tendency	878:895	a reduced tendency of desmosterol of segregating into ordered domains	878:946	Differential ordering effect of sterols in ternary-mixture model membranes revealed a reduced tendency of desmosterol of segregating into ordered domains.
32416195	5	78	theme	compatible	1074:1083	arg1	sub-populations					1058:1072	sperm sub-populations	1052:1072	sperm sub-populations compatible with different sterol contents	1052:1114	Live cell imaging of fluorescent cholesterol showed sterol incorporation and evidenced the presence of sperm sub-populations compatible with different sterol contents and a high concentration of sterol rich-ordered domains mainly at the acrosome plasma membrane.
32416195	7	79	theme	10 mM	1452:1456	arg1	MβCD-sterols					1458:1469	10 mM MβCD-sterols	1452:1469	10 mM MβCD-sterols prior to cryopreservation	1452:1495	Ram sperm incubation with 10 mM MβCD-sterols prior to cryopreservation in a cholesterol-free extender improved sperm quality parameters after cooling and freezing.
32416195	2	80	theme	-sterol	468:474	arg1	complexes					476:484	methyl-β-cyclodextrin (MβCD)-sterol complexes	440:484	methyl-β-cyclodextrin (MβCD)-sterol complexes	440:484	We evaluated the extent of cholesterol and desmosterol incorporation into ram sperm through incubation with increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes, and its effect on membrane biophysical properties, membrane lateral organization and cryopreservation outcome.
32416195	3	81	theme	membrane	711:718	arg1	order					726:730	membrane lipid order	711:730	membrane lipid order	711:730	Sterols were effectively incorporated into the sperm membrane at 10 and 25 mM MβCD-sterols, similarly increasing membrane lipid order at physiological temperature and during temperature decrease.
32416195	2	82	theme	MβCD	463:466	arg1	complexes					476:484	methyl-β-cyclodextrin (MβCD)-sterol complexes	440:484	methyl-β-cyclodextrin (MβCD)-sterol complexes	440:484	We evaluated the extent of cholesterol and desmosterol incorporation into ram sperm through incubation with increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes, and its effect on membrane biophysical properties, membrane lateral organization and cryopreservation outcome.
32416195	0	83	theme	ram	47:49	arg1	membrane					57:64	ram sperm membrane	47:64	ram sperm membrane before cryopreservation	47:88	Cholesterol and desmosterol incorporation into ram sperm membrane before cryopreservation: Effects on membrane biophysical properties and sperm quality.
32416195	5	84	theme	sterol	1144:1149	arg1	domains					1164:1170	sterol rich-ordered domains	1144:1170	sterol rich-ordered domains	1144:1170	Live cell imaging of fluorescent cholesterol showed sterol incorporation and evidenced the presence of sperm sub-populations compatible with different sterol contents and a high concentration of sterol rich-ordered domains mainly at the acrosome plasma membrane.
32416195	2	85	theme	methyl-β-cyclodextrin	440:460	arg1	complexes					476:484	methyl-β-cyclodextrin (MβCD)-sterol complexes	440:484	methyl-β-cyclodextrin (MβCD)-sterol complexes	440:484	We evaluated the extent of cholesterol and desmosterol incorporation into ram sperm through incubation with increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes, and its effect on membrane biophysical properties, membrane lateral organization and cryopreservation outcome.
32416195	9	86	dep	incorporation	1866:1878	arg1	the					1852:1854	the	1852:1854	the	1852:1854	Our research provides evidence on the effective incorporation and biophysical behavior of cholesterol and desmosterol in ram sperm membranes and on their consequences in improving functional parameters of sperm after temperature decrease and freezing.
32416195	5	87	theme	domains	1164:1170	arg1	concentration					1127:1139	a high concentration	1120:1139	a high concentration of sterol rich-ordered domains	1120:1170	Live cell imaging of fluorescent cholesterol showed sterol incorporation and evidenced the presence of sperm sub-populations compatible with different sterol contents and a high concentration of sterol rich-ordered domains mainly at the acrosome plasma membrane.
32416195	5	87	theme	domains	1164:1170	arg1	presence					1040:1047	the presence	1036:1047	the presence of sperm sub-populations compatible with different sterol contents	1036:1114	Live cell imaging of fluorescent cholesterol showed sterol incorporation and evidenced the presence of sperm sub-populations compatible with different sterol contents and a high concentration of sterol rich-ordered domains mainly at the acrosome plasma membrane.
32416195	7	88	theme	Ram	1426:1428	arg1	incubation					1436:1445	Ram sperm incubation	1426:1445	Ram sperm incubation with 10 mM MβCD-sterols prior to cryopreservation in a cholesterol-free extender	1426:1526	Ram sperm incubation with 10 mM MβCD-sterols prior to cryopreservation in a cholesterol-free extender improved sperm quality parameters after cooling and freezing.
32416195	8	89	theme	sperm	1784:1788	arg1	ability					1769:1775	the ability	1765:1775	the ability of ram sperm to overcome osmotic stress	1765:1815	While treatment with 10 mM MβCD-cholesterol increased sperm motility, membrane integrity and tolerance to osmotic stress after thawing, incorporation of desmosterol increased the ability of ram sperm to overcome osmotic stress.
32416195	3	90	theme	physiological	735:747	arg1	temperature					749:759	physiological temperature	735:759	physiological temperature	735:759	Sterols were effectively incorporated into the sperm membrane at 10 and 25 mM MβCD-sterols, similarly increasing membrane lipid order at physiological temperature and during temperature decrease.
32416195	4	91	from	effect	816:821	arg1	membranes					859:867	ternary-mixture model membranes	837:867	ternary-mixture model membranes	837:867	Differential ordering effect of sterols in ternary-mixture model membranes revealed a reduced tendency of desmosterol of segregating into ordered domains.
32416195	2	92	from	concentrations	422:435	arg1	properties					526:535	membrane biophysical properties	505:535	membrane biophysical properties	505:535	We evaluated the extent of cholesterol and desmosterol incorporation into ram sperm through incubation with increasing concentrations of methyl-β-cyclodextrin (MβCD)-sterol complexes, and its effect on membrane biophysical properties, membrane lateral organization and cryopreservation outcome.
32416195	4	93	theme	reduced	880:886	arg1	tendency					888:895	a reduced tendency	878:895	a reduced tendency of desmosterol of segregating into ordered domains	878:946	Differential ordering effect of sterols in ternary-mixture model membranes revealed a reduced tendency of desmosterol of segregating into ordered domains.
32416195	1	94	theme	lipid	228:232	arg1	composition					234:244	their lipid composition	222:244	their lipid composition	222:244	Ram sperm are particularly sensitive to freeze-thawing mainly due to their lipid composition, limiting their use in artificial insemination programs.
32416195	6	95	theme	membrane	1247:1254	arg1	organization					1220:1231	Lateral organization	1212:1231	Lateral organization	1212:1231	Lateral organization of the plasma membrane, assessed by identification of GM1-related rafts, was preserved after sterol incorporation except when high levels of sterols (25 mM MβCD-desmosterol) were incorporated.
32416195	3	96	theme	temperature	772:782	arg1	decrease					784:791	temperature decrease	772:791	temperature decrease	772:791	Sterols were effectively incorporated into the sperm membrane at 10 and 25 mM MβCD-sterols, similarly increasing membrane lipid order at physiological temperature and during temperature decrease.
32411618	13	0	contain	had	1926:1928	arg1	particles					1890:1898	the mHA/CS particles	1879:1898	the mHA/CS particles	1879:1898	The analyses showed that the mHA/CS particles were 2 μm in diameter and had a uniform pore size.
32411618	13	0	contain	had	1926:1928	arg1	μm					1907:1908	2 μm	1905:1908	2 μm in diameter	1905:1920	The analyses showed that the mHA/CS particles were 2 μm in diameter and had a uniform pore size.
32411618	13	0	contain	had	1926:1928	arg2	size					1945:1948	a uniform pore size	1930:1948	a uniform pore size	1930:1948	The analyses showed that the mHA/CS particles were 2 μm in diameter and had a uniform pore size.
32411618	7	1	theme	baseline	1119:1126	arg1	medium					1098:1103	Tryptic Soy Broth culture medium	1072:1103	Tryptic Soy Broth culture medium	1072:1103	Tryptic Soy Broth culture medium was used as a baseline control.
32411618	7	1	theme	baseline	1119:1126	arg1	control					1128:1134	a baseline control	1117:1134	a baseline control	1117:1134	Tryptic Soy Broth culture medium was used as a baseline control.
32411618	8	2	theme	rhAm	1158:1161	arg1	effect					1148:1153	Osteogenic effect	1137:1153	Osteogenic effect of rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm) on human periodontal ligament cells (hPDLCs)	1137:1303	Osteogenic effect of rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm) on human periodontal ligament cells (hPDLCs) was evaluated in osteogenic media.
32411618	12	3	used	used	1824:1827	arg2	slices					1775:1780	The root slices	1766:1780	The root slices treated with osteogenic medium alone	1766:1817	The root slices treated with osteogenic medium alone were used as the baseline control.
32411618	12	3	used	used	1824:1827	arg2	control					1845:1851	the baseline control	1832:1851	the baseline control	1832:1851	The root slices treated with osteogenic medium alone were used as the baseline control.
32411618	16	4	theme	mHA/CS	2145:2150	arg1	scaffold					2152:2159	The mHA/CS scaffold	2141:2159	The mHA/CS scaffold	2141:2159	The mHA/CS scaffold sustainably released rhAm.
32411618	13	5	theme	pore	1940:1943	arg1	size					1945:1948	a uniform pore size	1930:1948	a uniform pore size	1930:1948	The analyses showed that the mHA/CS particles were 2 μm in diameter and had a uniform pore size.
32411618	17	6	theme	ALP	2237:2239	arg1	activity					2241:2248	upregulated ALP activity	2225:2248	upregulated ALP activity	2225:2248	The mHA/CS loaded with 20 μg/mL rhAm upregulated ALP activity, the expression levels of osteogenesis-related genes and proteins in vitro.
32411618	9	7	theme	osteogenic	1371:1380	arg1	media					1382:1386	osteogenic media	1371:1386	osteogenic media	1371:1386	The hPDLCs treated either with osteogenic media or Dulbecco's modified Eagle's medium (DMEM) alone were used as the baseline control.
32411618	12	8	theme	root	1770:1773	arg1	slices					1775:1780	The root slices	1766:1780	The root slices treated with osteogenic medium alone	1766:1817	The root slices treated with osteogenic medium alone were used as the baseline control.
32411618	12	8	theme	root	1770:1773	arg1	control					1845:1851	the baseline control	1832:1851	the baseline control	1832:1851	The root slices treated with osteogenic medium alone were used as the baseline control.
32411618	4	9	theme	Brunauer-Emmett-Teller	717:738	arg1	analysis					746:753	Brunauer-Emmett-Teller (BET) analysis	717:753	Brunauer-Emmett-Teller (BET) analysis	717:753	The physicochemical properties of mHA/CS scaffolds were examined by Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and Brunauer-Emmett-Teller (BET) analysis.
32411618	10	10	theme	4-week-old	1495:1504	arg1	BALB/c					1517:1522	BALB/c	1517:1522	BALB/c	1517:1522	In the animal model, 4-week-old nude mice (BALB/c) (n = 6) implanted with root slices subcutaneously were used to observe the cementogenic effect in vivo.
32411618	10	10	theme	4-week-old	1495:1504	arg1	n					1526:1526	n = 6	1526:1530	n = 6	1526:1530	In the animal model, 4-week-old nude mice (BALB/c) (n = 6) implanted with root slices subcutaneously were used to observe the cementogenic effect in vivo.
32411618	10	10	theme	4-week-old	1495:1504	arg1	mice					1511:1514	4-week-old nude mice	1495:1514	4-week-old nude mice (BALB/c) (n = 6) implanted with root slices subcutaneously	1495:1573	In the animal model, 4-week-old nude mice (BALB/c) (n = 6) implanted with root slices subcutaneously were used to observe the cementogenic effect in vivo.
32411618	7	11	theme	culture	1090:1096	arg1	medium					1098:1103	Tryptic Soy Broth culture medium	1072:1103	Tryptic Soy Broth culture medium	1072:1103	Tryptic Soy Broth culture medium was used as a baseline control.
32411618	7	11	theme	culture	1090:1096	arg1	control					1128:1134	a baseline control	1117:1134	a baseline control	1117:1134	Tryptic Soy Broth culture medium was used as a baseline control.
32411618	2	12	theme	periodontal	348:358	arg1	regeneration					360:371	periodontal regeneration	348:371	periodontal regeneration	348:371	This study was the first to apply the mesoporous hydroxyapatites/chitosan (mHA/CS) composite scaffold to periodontal regeneration.
32411618	19	13	theme	bone	2537:2540	arg1	formation					2524:2532	the formation	2520:2532	the formation of bone and cementum-like tissue	2520:2565	Our findings suggest that mHA/CS loaded with 20 μg/mL rhAm could inhibit the growth of periodontal pathogens and promote the formation of bone and cementum-like tissue.
32411618	17	14	theme	expression	2255:2264	arg1	levels					2266:2271	the expression levels	2251:2271	the expression levels of osteogenesis-related genes	2251:2301	The mHA/CS loaded with 20 μg/mL rhAm upregulated ALP activity, the expression levels of osteogenesis-related genes and proteins in vitro.
32411618	7	15	theme	Soy	1080:1082	arg1	medium					1098:1103	Tryptic Soy Broth culture medium	1072:1103	Tryptic Soy Broth culture medium	1072:1103	Tryptic Soy Broth culture medium was used as a baseline control.
32411618	7	15	theme	Soy	1080:1082	arg1	control					1128:1134	a baseline control	1117:1134	a baseline control	1117:1134	Tryptic Soy Broth culture medium was used as a baseline control.
32411618	6	16	theme	CS	1000:1001	arg1	mg/mL					1040:1044	1.5 mg/mL CS; 3 mg/mL mHA; 2.25 mg/mL mHA/CS; 4.5 mg/mL mHA/CS and 20 μg/mL rhAm	990:1069	mg/mL	1040:1044	The antibacterial effect was tested on 1.5 mg/mL CS; 3 mg/mL mHA; 2.25 mg/mL mHA/CS; 4.5 mg/mL mHA/CS and 20 μg/mL rhAm.
32411618	18	17	theme	cementum-like	2369:2381	arg1	tissue					2383:2388	cementum-like tissue	2369:2388	cementum-like tissue	2369:2388	Additionally, it promoted the formation of cementum-like tissue in vivo.
32411618	9	18	theme	Eagle	1411:1415	arg1	DMEM					1427:1430	DMEM	1427:1430	DMEM	1427:1430	The hPDLCs treated either with osteogenic media or Dulbecco's modified Eagle's medium (DMEM) alone were used as the baseline control.
32411618	9	18	theme	Eagle	1411:1415	arg1	medium					1419:1424	Dulbecco's modified Eagle's medium	1391:1424	Dulbecco's modified Eagle's medium (DMEM)	1391:1431	The hPDLCs treated either with osteogenic media or Dulbecco's modified Eagle's medium (DMEM) alone were used as the baseline control.
32411618	8	19	theme	mg/mL	1192:1196	arg1	mHA/CS					1198:1203	4.5 mg/mL mHA/CS	1188:1203	4.5 mg/mL mHA/CS	1188:1203	Osteogenic effect of rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm) on human periodontal ligament cells (hPDLCs) was evaluated in osteogenic media.
32411618	8	19	theme	mg/mL	1192:1196	arg1	mHA/CS					1180:1185	mHA/CS	1180:1185	mHA/CS (4.5 mg/mL mHA/CS)	1180:1204	Osteogenic effect of rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm) on human periodontal ligament cells (hPDLCs) was evaluated in osteogenic media.
32411618	12	20	theme	osteogenic	1795:1804	arg1	medium					1806:1811	osteogenic medium	1795:1811	osteogenic medium	1795:1811	The root slices treated with osteogenic medium alone were used as the baseline control.
32411618	13	21	theme	mHA/CS	1883:1888	arg1	particles					1890:1898	the mHA/CS particles	1879:1898	the mHA/CS particles	1879:1898	The analyses showed that the mHA/CS particles were 2 μm in diameter and had a uniform pore size.
32411618	13	21	theme	mHA/CS	1883:1888	arg1	μm					1907:1908	2 μm	1905:1908	2 μm in diameter	1905:1920	The analyses showed that the mHA/CS particles were 2 μm in diameter and had a uniform pore size.
32411618	9	22	used	used	1444:1447	arg2	hPDLCs					1344:1349	The hPDLCs	1340:1349	The hPDLCs treated either with osteogenic media or Dulbecco's modified Eagle's medium (DMEM) alone	1340:1437	The hPDLCs treated either with osteogenic media or Dulbecco's modified Eagle's medium (DMEM) alone were used as the baseline control.
32411618	9	22	used	used	1444:1447	arg2	control					1465:1471	the baseline control	1452:1471	the baseline control	1452:1471	The hPDLCs treated either with osteogenic media or Dulbecco's modified Eagle's medium (DMEM) alone were used as the baseline control.
32411618	8	23	theme	μg/mL	1248:1252	arg1	mHA/CS-rhAm					1211:1221	mHA/CS-rhAm	1211:1221	mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm)	1211:1258	Osteogenic effect of rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm) on human periodontal ligament cells (hPDLCs) was evaluated in osteogenic media.
32411618	8	23	theme	μg/mL	1248:1252	arg1	rhAm					1254:1257	20 μg/mL rhAm	1245:1257	20 μg/mL rhAm	1245:1257	Osteogenic effect of rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm) on human periodontal ligament cells (hPDLCs) was evaluated in osteogenic media.
32411618	2	24	theme	mesoporous	281:290	arg1	scaffold					336:343	the mesoporous hydroxyapatites/chitosan (mHA/CS) composite scaffold	277:343	the mesoporous hydroxyapatites/chitosan (mHA/CS) composite scaffold to periodontal regeneration	277:371	This study was the first to apply the mesoporous hydroxyapatites/chitosan (mHA/CS) composite scaffold to periodontal regeneration.
32411618	1	25	theme	periodontium	174:185	arg1	recovery					153:160	The recovery	149:160	The recovery of impaired periodontium	149:185	The recovery of impaired periodontium is still a challenge to the treatment of periodontitis.
32411618	1	25	theme	periodontium	174:185	arg1	challenge					198:206	a challenge	196:206	a challenge to the treatment of periodontitis	196:240	The recovery of impaired periodontium is still a challenge to the treatment of periodontitis.
32411618	4	26	theme	BET	741:743	arg1	analysis					746:753	Brunauer-Emmett-Teller (BET) analysis	717:753	Brunauer-Emmett-Teller (BET) analysis	717:753	The physicochemical properties of mHA/CS scaffolds were examined by Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and Brunauer-Emmett-Teller (BET) analysis.
32411618	0	27	theme	Antibacterial	90:102	arg1	Effect					104:109	Antibacterial Effect	90:109	Antibacterial Effect	90:109	Mesoporous Hydroxyapatite/Chitosan Loaded With Recombinant-Human Amelogenin Could Enhance Antibacterial Effect and Promote Periodontal Regeneration.
32411618	6	28	theme	mHA/CS	1028:1033	arg1	mg/mL					1040:1044	1.5 mg/mL CS; 3 mg/mL mHA; 2.25 mg/mL mHA/CS; 4.5 mg/mL mHA/CS and 20 μg/mL rhAm	990:1069	mg/mL	1040:1044	The antibacterial effect was tested on 1.5 mg/mL CS; 3 mg/mL mHA; 2.25 mg/mL mHA/CS; 4.5 mg/mL mHA/CS and 20 μg/mL rhAm.
32411618	8	29	theme	human	1263:1267	arg1	cells					1290:1294	human periodontal ligament cells	1263:1294	human periodontal ligament cells (hPDLCs)	1263:1303	Osteogenic effect of rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm) on human periodontal ligament cells (hPDLCs) was evaluated in osteogenic media.
32411618	8	29	theme	human	1263:1267	arg1	hPDLCs					1297:1302	hPDLCs	1297:1302	hPDLCs	1297:1302	Osteogenic effect of rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm) on human periodontal ligament cells (hPDLCs) was evaluated in osteogenic media.
32411618	15	30	dep	Fusobacterium	2087:2099	arg1	nucleatum					2101:2109	Fusobacterium nucleatum	2087:2109	Fusobacterium nucleatum	2087:2109	The scaffold exhibited antibacterial effects against Fusobacterium nucleatum and Porphyromonas gingivalis.
32411618	8	31	theme	ligament	1281:1288	arg1	cells					1290:1294	human periodontal ligament cells	1263:1294	human periodontal ligament cells (hPDLCs)	1263:1303	Osteogenic effect of rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm) on human periodontal ligament cells (hPDLCs) was evaluated in osteogenic media.
32411618	8	31	theme	ligament	1281:1288	arg1	hPDLCs					1297:1302	hPDLCs	1297:1302	hPDLCs	1297:1302	Osteogenic effect of rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm) on human periodontal ligament cells (hPDLCs) was evaluated in osteogenic media.
32411618	0	32	theme	Periodontal	123:133	arg1	Regeneration					135:146	Periodontal Regeneration	123:146	Periodontal Regeneration	123:146	Mesoporous Hydroxyapatite/Chitosan Loaded With Recombinant-Human Amelogenin Could Enhance Antibacterial Effect and Promote Periodontal Regeneration.
32411618	4	33	dep	Fourier	625:631	arg1	transform					633:641	transform	633:641	transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and Brunauer-Emmett-Teller (BET) analysis	633:753	The physicochemical properties of mHA/CS scaffolds were examined by Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and Brunauer-Emmett-Teller (BET) analysis.
32411618	19	34	theme	μg/mL	2447:2451	arg1	rhAm					2453:2456	20 μg/mL rhAm	2444:2456	20 μg/mL rhAm	2444:2456	Our findings suggest that mHA/CS loaded with 20 μg/mL rhAm could inhibit the growth of periodontal pathogens and promote the formation of bone and cementum-like tissue.
32411618	6	35	theme	mHA	1012:1014	arg1	mg/mL					1040:1044	1.5 mg/mL CS; 3 mg/mL mHA; 2.25 mg/mL mHA/CS; 4.5 mg/mL mHA/CS and 20 μg/mL rhAm	990:1069	mg/mL	1040:1044	The antibacterial effect was tested on 1.5 mg/mL CS; 3 mg/mL mHA; 2.25 mg/mL mHA/CS; 4.5 mg/mL mHA/CS and 20 μg/mL rhAm.
32411618	15	36	theme	antibacterial	2057:2069	arg1	effects					2071:2077	antibacterial effects	2057:2077	antibacterial effects	2057:2077	The scaffold exhibited antibacterial effects against Fusobacterium nucleatum and Porphyromonas gingivalis.
32411618	14	37	from	m2/g	2028:2031	arg1	diameter					1988:1995	diameter	1988:1995	diameter	1988:1995	The mesoporous structure was 7 nm in diameter and its surface area was 33.95 m2/g.
32411618	3	38	theme	biological	414:423	arg1	effects					425:431	the biological effects	410:431	the biological effects of mesoporous hydroxyapatite/chitosan (mHA/CS) loaded with recombinant human amelogenin (rhAm) on periodontal regeneration	410:554	The aim of our study is to evaluate the biological effects of mesoporous hydroxyapatite/chitosan (mHA/CS) loaded with recombinant human amelogenin (rhAm) on periodontal regeneration.
32411618	3	39	theme	mHA/CS	472:477	arg1	effects					425:431	the biological effects	410:431	the biological effects of mesoporous hydroxyapatite/chitosan (mHA/CS) loaded with recombinant human amelogenin (rhAm) on periodontal regeneration	410:554	The aim of our study is to evaluate the biological effects of mesoporous hydroxyapatite/chitosan (mHA/CS) loaded with recombinant human amelogenin (rhAm) on periodontal regeneration.
32411618	8	40	theme	osteogenic	1322:1331	arg1	media					1333:1337	osteogenic media	1322:1337	osteogenic media	1322:1337	Osteogenic effect of rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm) on human periodontal ligament cells (hPDLCs) was evaluated in osteogenic media.
32411618	10	41	theme	root	1548:1551	arg1	slices					1553:1558	root slices	1548:1558	root slices	1548:1558	In the animal model, 4-week-old nude mice (BALB/c) (n = 6) implanted with root slices subcutaneously were used to observe the cementogenic effect in vivo.
32411618	5	42	theme	controlled-release	864:881	arg1	effect					856:861	antibacterial effect	842:861	antibacterial effect	842:861	Then, the biological effects of the mHA/CS loaded with rhAm were evaluated, including antibacterial effect, controlled-release capacity, osteogenic and cementogenic effects in vitro and in vivo.
32411618	5	42	theme	controlled-release	864:881	arg1	capacity					883:890	controlled-release capacity	864:890	controlled-release capacity	864:890	Then, the biological effects of the mHA/CS loaded with rhAm were evaluated, including antibacterial effect, controlled-release capacity, osteogenic and cementogenic effects in vitro and in vivo.
32411618	8	43	from	effect	1148:1153	arg1	cells					1290:1294	human periodontal ligament cells	1263:1294	human periodontal ligament cells (hPDLCs)	1263:1303	Osteogenic effect of rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm) on human periodontal ligament cells (hPDLCs) was evaluated in osteogenic media.
32411618	8	43	from	effect	1148:1153	arg1	hPDLCs					1297:1302	hPDLCs	1297:1302	hPDLCs	1297:1302	Osteogenic effect of rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm) on human periodontal ligament cells (hPDLCs) was evaluated in osteogenic media.
32411618	6	44	theme	20	1057:1058	arg1	μg/mL					1060:1064	μg/mL	1060:1064	μg/mL	1060:1064	The antibacterial effect was tested on 1.5 mg/mL CS; 3 mg/mL mHA; 2.25 mg/mL mHA/CS; 4.5 mg/mL mHA/CS and 20 μg/mL rhAm.
32411618	3	45	theme	hydroxyapatite/chitosan	447:469	arg1	mHA/CS					472:477	mesoporous hydroxyapatite/chitosan (mHA/CS)	436:478	mesoporous hydroxyapatite/chitosan (mHA/CS) loaded with recombinant human amelogenin (rhAm)	436:526	The aim of our study is to evaluate the biological effects of mesoporous hydroxyapatite/chitosan (mHA/CS) loaded with recombinant human amelogenin (rhAm) on periodontal regeneration.
32411618	9	46	dep	media	1382:1386	arg1	either					1359:1364	either	1359:1364	either	1359:1364	The hPDLCs treated either with osteogenic media or Dulbecco's modified Eagle's medium (DMEM) alone were used as the baseline control.
32411618	14	47	theme	surface	2005:2011	arg1	area					2013:2016	its surface area	2001:2016	its surface area	2001:2016	The mesoporous structure was 7 nm in diameter and its surface area was 33.95 m2/g.
32411618	3	48	theme	recombinant	492:502	arg1	rhAm					522:525	rhAm	522:525	rhAm	522:525	The aim of our study is to evaluate the biological effects of mesoporous hydroxyapatite/chitosan (mHA/CS) loaded with recombinant human amelogenin (rhAm) on periodontal regeneration.
32411618	3	48	theme	recombinant	492:502	arg1	amelogenin					510:519	recombinant human amelogenin	492:519	recombinant human amelogenin (rhAm)	492:526	The aim of our study is to evaluate the biological effects of mesoporous hydroxyapatite/chitosan (mHA/CS) loaded with recombinant human amelogenin (rhAm) on periodontal regeneration.
32411618	0	49	theme	Recombinant-Human	47:63	arg1	Amelogenin					65:74	Recombinant-Human Amelogenin	47:74	Recombinant-Human Amelogenin	47:74	Mesoporous Hydroxyapatite/Chitosan Loaded With Recombinant-Human Amelogenin Could Enhance Antibacterial Effect and Promote Periodontal Regeneration.
32411618	19	50	theme	pathogens	2498:2506	arg1	growth					2476:2481	the growth	2472:2481	the growth of periodontal pathogens	2472:2506	Our findings suggest that mHA/CS loaded with 20 μg/mL rhAm could inhibit the growth of periodontal pathogens and promote the formation of bone and cementum-like tissue.
32411618	17	51	theme	osteogenesis-related	2276:2295	arg1	genes					2297:2301	osteogenesis-related genes	2276:2301	osteogenesis-related genes	2276:2301	The mHA/CS loaded with 20 μg/mL rhAm upregulated ALP activity, the expression levels of osteogenesis-related genes and proteins in vitro.
32411618	4	52	theme	mHA/CS	591:596	arg1	scaffolds					598:606	mHA/CS scaffolds	591:606	mHA/CS scaffolds	591:606	The physicochemical properties of mHA/CS scaffolds were examined by Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and Brunauer-Emmett-Teller (BET) analysis.
32411618	10	53	theme	cementogenic	1600:1611	arg1	effect					1613:1618	the cementogenic effect	1596:1618	the cementogenic effect	1596:1618	In the animal model, 4-week-old nude mice (BALB/c) (n = 6) implanted with root slices subcutaneously were used to observe the cementogenic effect in vivo.
32411618	19	54	theme	cementum-like	2546:2558	arg1	tissue					2560:2565	cementum-like tissue	2546:2565	cementum-like tissue	2546:2565	Our findings suggest that mHA/CS loaded with 20 μg/mL rhAm could inhibit the growth of periodontal pathogens and promote the formation of bone and cementum-like tissue.
32411618	7	55	used	used	1109:1112	arg2	control					1128:1134	a baseline control	1117:1134	a baseline control	1117:1134	Tryptic Soy Broth culture medium was used as a baseline control.
32411618	7	55	used	used	1109:1112	arg2	medium					1098:1103	Tryptic Soy Broth culture medium	1072:1103	Tryptic Soy Broth culture medium	1072:1103	Tryptic Soy Broth culture medium was used as a baseline control.
32411618	10	56	used	used	1580:1583	arg2	BALB/c					1517:1522	BALB/c	1517:1522	BALB/c	1517:1522	In the animal model, 4-week-old nude mice (BALB/c) (n = 6) implanted with root slices subcutaneously were used to observe the cementogenic effect in vivo.
32411618	10	56	used	used	1580:1583	arg2	mice					1511:1514	4-week-old nude mice	1495:1514	4-week-old nude mice (BALB/c) (n = 6) implanted with root slices subcutaneously	1495:1573	In the animal model, 4-week-old nude mice (BALB/c) (n = 6) implanted with root slices subcutaneously were used to observe the cementogenic effect in vivo.
32411618	10	56	used	used	1580:1583	arg2	n					1526:1526	n = 6	1526:1530	n = 6	1526:1530	In the animal model, 4-week-old nude mice (BALB/c) (n = 6) implanted with root slices subcutaneously were used to observe the cementogenic effect in vivo.
32411618	14	57	theme	mesoporous	1955:1964	arg1	structure					1966:1974	The mesoporous structure	1951:1974	The mesoporous structure	1951:1974	The mesoporous structure was 7 nm in diameter and its surface area was 33.95 m2/g.
32411618	14	57	theme	mesoporous	1955:1964	arg1	nm					1982:1983	7 nm	1980:1983	7 nm	1980:1983	The mesoporous structure was 7 nm in diameter and its surface area was 33.95 m2/g.
32411618	11	58	theme	mg/mL	1697:1701	arg1	mHA/CS					1703:1708	4.5 mg/mL mHA/CS	1693:1708	4.5 mg/mL mHA/CS	1693:1708	The root slices were treated with rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm).
32411618	11	58	theme	mg/mL	1697:1701	arg1	mHA/CS					1685:1690	mHA/CS	1685:1690	mHA/CS (4.5 mg/mL mHA/CS)	1685:1709	The root slices were treated with rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm).
32411618	8	59	theme	Osteogenic	1137:1146	arg1	effect					1148:1153	Osteogenic effect	1137:1153	Osteogenic effect of rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm) on human periodontal ligament cells (hPDLCs)	1137:1303	Osteogenic effect of rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm) on human periodontal ligament cells (hPDLCs) was evaluated in osteogenic media.
32411618	6	60	theme	antibacterial	955:967	arg1	effect					969:974	The antibacterial effect	951:974	The antibacterial effect	951:974	The antibacterial effect was tested on 1.5 mg/mL CS; 3 mg/mL mHA; 2.25 mg/mL mHA/CS; 4.5 mg/mL mHA/CS and 20 μg/mL rhAm.
32411618	10	61	theme	animal	1481:1486	arg1	model					1488:1492	the animal model	1477:1492	the animal model	1477:1492	In the animal model, 4-week-old nude mice (BALB/c) (n = 6) implanted with root slices subcutaneously were used to observe the cementogenic effect in vivo.
32411618	2	62	theme	composite	326:334	arg1	scaffold					336:343	the mesoporous hydroxyapatites/chitosan (mHA/CS) composite scaffold	277:343	the mesoporous hydroxyapatites/chitosan (mHA/CS) composite scaffold to periodontal regeneration	277:371	This study was the first to apply the mesoporous hydroxyapatites/chitosan (mHA/CS) composite scaffold to periodontal regeneration.
32411618	13	63	theme	uniform	1932:1938	arg1	size					1945:1948	a uniform pore size	1930:1948	a uniform pore size	1930:1948	The analyses showed that the mHA/CS particles were 2 μm in diameter and had a uniform pore size.
32411618	17	64	theme	upregulated	2225:2235	arg1	activity					2241:2248	upregulated ALP activity	2225:2248	upregulated ALP activity	2225:2248	The mHA/CS loaded with 20 μg/mL rhAm upregulated ALP activity, the expression levels of osteogenesis-related genes and proteins in vitro.
32411618	13	65	from	μm	1907:1908	arg1	diameter					1913:1920	diameter	1913:1920	diameter	1913:1920	The analyses showed that the mHA/CS particles were 2 μm in diameter and had a uniform pore size.
32411618	2	66	theme	mHA/CS	318:323	arg1	scaffold					336:343	the mesoporous hydroxyapatites/chitosan (mHA/CS) composite scaffold	277:343	the mesoporous hydroxyapatites/chitosan (mHA/CS) composite scaffold to periodontal regeneration	277:371	This study was the first to apply the mesoporous hydroxyapatites/chitosan (mHA/CS) composite scaffold to periodontal regeneration.
32411618	5	67	theme	cementogenic	908:919	arg1	effects					921:927	osteogenic and cementogenic effects	893:927	osteogenic and cementogenic effects in vitro and in vivo	893:948	Then, the biological effects of the mHA/CS loaded with rhAm were evaluated, including antibacterial effect, controlled-release capacity, osteogenic and cementogenic effects in vitro and in vivo.
32411618	5	67	theme	cementogenic	908:919	arg1	effect					856:861	antibacterial effect	842:861	antibacterial effect	842:861	Then, the biological effects of the mHA/CS loaded with rhAm were evaluated, including antibacterial effect, controlled-release capacity, osteogenic and cementogenic effects in vitro and in vivo.
32411618	10	68	theme	nude	1506:1509	arg1	BALB/c					1517:1522	BALB/c	1517:1522	BALB/c	1517:1522	In the animal model, 4-week-old nude mice (BALB/c) (n = 6) implanted with root slices subcutaneously were used to observe the cementogenic effect in vivo.
32411618	10	68	theme	nude	1506:1509	arg1	n					1526:1526	n = 6	1526:1530	n = 6	1526:1530	In the animal model, 4-week-old nude mice (BALB/c) (n = 6) implanted with root slices subcutaneously were used to observe the cementogenic effect in vivo.
32411618	10	68	theme	nude	1506:1509	arg1	mice					1511:1514	4-week-old nude mice	1495:1514	4-week-old nude mice (BALB/c) (n = 6) implanted with root slices subcutaneously	1495:1573	In the animal model, 4-week-old nude mice (BALB/c) (n = 6) implanted with root slices subcutaneously were used to observe the cementogenic effect in vivo.
32411618	7	69	theme	Broth	1084:1088	arg1	medium					1098:1103	Tryptic Soy Broth culture medium	1072:1103	Tryptic Soy Broth culture medium	1072:1103	Tryptic Soy Broth culture medium was used as a baseline control.
32411618	7	69	theme	Broth	1084:1088	arg1	control					1128:1134	a baseline control	1117:1134	a baseline control	1117:1134	Tryptic Soy Broth culture medium was used as a baseline control.
32411618	8	70	theme	μg/mL	1167:1171	arg1	rhAm					1158:1161	rhAm	1158:1161	rhAm (20 μg/mL rhAm)	1158:1177	Osteogenic effect of rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm) on human periodontal ligament cells (hPDLCs) was evaluated in osteogenic media.
32411618	8	70	theme	μg/mL	1167:1171	arg1	rhAm					1173:1176	20 μg/mL rhAm	1164:1176	20 μg/mL rhAm	1164:1176	Osteogenic effect of rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm) on human periodontal ligament cells (hPDLCs) was evaluated in osteogenic media.
32411618	6	71	theme	mg/mL	1006:1010	arg1	mg/mL					1040:1044	1.5 mg/mL CS; 3 mg/mL mHA; 2.25 mg/mL mHA/CS; 4.5 mg/mL mHA/CS and 20 μg/mL rhAm	990:1069	mg/mL	1040:1044	The antibacterial effect was tested on 1.5 mg/mL CS; 3 mg/mL mHA; 2.25 mg/mL mHA/CS; 4.5 mg/mL mHA/CS and 20 μg/mL rhAm.
32411618	7	72	theme	Tryptic	1072:1078	arg1	medium					1098:1103	Tryptic Soy Broth culture medium	1072:1103	Tryptic Soy Broth culture medium	1072:1103	Tryptic Soy Broth culture medium was used as a baseline control.
32411618	7	72	theme	Tryptic	1072:1078	arg1	control					1128:1134	a baseline control	1117:1134	a baseline control	1117:1134	Tryptic Soy Broth culture medium was used as a baseline control.
32411618	1	73	theme	periodontitis	228:240	arg1	treatment					215:223	the treatment	211:223	the treatment of periodontitis	211:240	The recovery of impaired periodontium is still a challenge to the treatment of periodontitis.
32411618	8	74	theme	mHA/CS-rhAm	1211:1221	arg1	effect					1148:1153	Osteogenic effect	1137:1153	Osteogenic effect of rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm) on human periodontal ligament cells (hPDLCs)	1137:1303	Osteogenic effect of rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm) on human periodontal ligament cells (hPDLCs) was evaluated in osteogenic media.
32411618	18	75	theme	tissue	2383:2388	arg1	formation					2356:2364	the formation	2352:2364	the formation of cementum-like tissue in vivo	2352:2396	Additionally, it promoted the formation of cementum-like tissue in vivo.
32411618	5	76	theme	osteogenic	893:902	arg1	effects					921:927	osteogenic and cementogenic effects	893:927	osteogenic and cementogenic effects in vitro and in vivo	893:948	Then, the biological effects of the mHA/CS loaded with rhAm were evaluated, including antibacterial effect, controlled-release capacity, osteogenic and cementogenic effects in vitro and in vivo.
32411618	5	76	theme	osteogenic	893:902	arg1	effect					856:861	antibacterial effect	842:861	antibacterial effect	842:861	Then, the biological effects of the mHA/CS loaded with rhAm were evaluated, including antibacterial effect, controlled-release capacity, osteogenic and cementogenic effects in vitro and in vivo.
32411618	8	77	theme	mHA/CS	1180:1185	arg1	effect					1148:1153	Osteogenic effect	1137:1153	Osteogenic effect of rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm) on human periodontal ligament cells (hPDLCs)	1137:1303	Osteogenic effect of rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm) on human periodontal ligament cells (hPDLCs) was evaluated in osteogenic media.
32411618	6	78	theme	mg/mL	994:998	arg1	mg/mL					1040:1044	1.5 mg/mL CS; 3 mg/mL mHA; 2.25 mg/mL mHA/CS; 4.5 mg/mL mHA/CS and 20 μg/mL rhAm	990:1069	mg/mL	1040:1044	The antibacterial effect was tested on 1.5 mg/mL CS; 3 mg/mL mHA; 2.25 mg/mL mHA/CS; 4.5 mg/mL mHA/CS and 20 μg/mL rhAm.
32411618	4	79	dep	transform	633:641	arg1	infrared					643:650	infrared	643:650	transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and Brunauer-Emmett-Teller (BET) analysis	633:753	The physicochemical properties of mHA/CS scaffolds were examined by Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and Brunauer-Emmett-Teller (BET) analysis.
32411618	11	80	theme	μg/mL	1753:1757	arg1	rhAm					1759:1762	20 μg/mL rhAm	1750:1762	20 μg/mL rhAm	1750:1762	The root slices were treated with rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm).
32411618	11	80	theme	μg/mL	1753:1757	arg1	mHA/CS-rhAm					1716:1726	mHA/CS-rhAm	1716:1726	mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm)	1716:1763	The root slices were treated with rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm).
32411618	11	81	theme	μg/mL	1672:1676	arg1	rhAm					1663:1666	rhAm	1663:1666	rhAm (20 μg/mL rhAm)	1663:1682	The root slices were treated with rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm).
32411618	11	81	theme	μg/mL	1672:1676	arg1	rhAm					1678:1681	20 μg/mL rhAm	1669:1681	20 μg/mL rhAm	1669:1681	The root slices were treated with rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm).
32411618	2	82	theme	hydroxyapatites/chitosan	292:315	arg1	scaffold					336:343	the mesoporous hydroxyapatites/chitosan (mHA/CS) composite scaffold	277:343	the mesoporous hydroxyapatites/chitosan (mHA/CS) composite scaffold to periodontal regeneration	277:371	This study was the first to apply the mesoporous hydroxyapatites/chitosan (mHA/CS) composite scaffold to periodontal regeneration.
32411618	1	83	theme	impaired	165:172	arg1	periodontium					174:185	impaired periodontium	165:185	impaired periodontium	165:185	The recovery of impaired periodontium is still a challenge to the treatment of periodontitis.
32411618	8	84	theme	20	1245:1246	arg1	μg/mL					1248:1252	μg/mL	1248:1252	μg/mL	1248:1252	Osteogenic effect of rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm) on human periodontal ligament cells (hPDLCs) was evaluated in osteogenic media.
32411618	5	85	theme	mHA/CS	792:797	arg1	effects					777:783	the biological effects	762:783	the biological effects of the mHA/CS loaded with rhAm	762:814	Then, the biological effects of the mHA/CS loaded with rhAm were evaluated, including antibacterial effect, controlled-release capacity, osteogenic and cementogenic effects in vitro and in vivo.
32411618	12	86	theme	baseline	1836:1843	arg1	slices					1775:1780	The root slices	1766:1780	The root slices treated with osteogenic medium alone	1766:1817	The root slices treated with osteogenic medium alone were used as the baseline control.
32411618	12	86	theme	baseline	1836:1843	arg1	control					1845:1851	the baseline control	1832:1851	the baseline control	1832:1851	The root slices treated with osteogenic medium alone were used as the baseline control.
32411618	6	87	theme	mg/mL	1022:1026	arg1	mg/mL					1040:1044	1.5 mg/mL CS; 3 mg/mL mHA; 2.25 mg/mL mHA/CS; 4.5 mg/mL mHA/CS and 20 μg/mL rhAm	990:1069	mg/mL	1040:1044	The antibacterial effect was tested on 1.5 mg/mL CS; 3 mg/mL mHA; 2.25 mg/mL mHA/CS; 4.5 mg/mL mHA/CS and 20 μg/mL rhAm.
32411618	4	88	theme	physicochemical	561:575	arg1	properties					577:586	The physicochemical properties	557:586	The physicochemical properties of mHA/CS scaffolds	557:606	The physicochemical properties of mHA/CS scaffolds were examined by Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and Brunauer-Emmett-Teller (BET) analysis.
32411618	5	89	theme	biological	766:775	arg1	effects					777:783	the biological effects	762:783	the biological effects of the mHA/CS loaded with rhAm	762:814	Then, the biological effects of the mHA/CS loaded with rhAm were evaluated, including antibacterial effect, controlled-release capacity, osteogenic and cementogenic effects in vitro and in vivo.
32411618	8	90	dep	mg/mL	1228:1232	arg1	mHA/CS					1234:1239	mHA/CS	1234:1239	mHA/CS	1234:1239	Osteogenic effect of rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm) on human periodontal ligament cells (hPDLCs) was evaluated in osteogenic media.
32411618	14	91	from	nm	1982:1983	arg1	diameter					1988:1995	diameter	1988:1995	diameter	1988:1995	The mesoporous structure was 7 nm in diameter and its surface area was 33.95 m2/g.
32411618	19	92	theme	tissue	2560:2565	arg1	formation					2524:2532	the formation	2520:2532	the formation of bone and cementum-like tissue	2520:2565	Our findings suggest that mHA/CS loaded with 20 μg/mL rhAm could inhibit the growth of periodontal pathogens and promote the formation of bone and cementum-like tissue.
32411618	8	93	theme	periodontal	1269:1279	arg1	cells					1290:1294	human periodontal ligament cells	1263:1294	human periodontal ligament cells (hPDLCs)	1263:1303	Osteogenic effect of rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm) on human periodontal ligament cells (hPDLCs) was evaluated in osteogenic media.
32411618	8	93	theme	periodontal	1269:1279	arg1	hPDLCs					1297:1302	hPDLCs	1297:1302	hPDLCs	1297:1302	Osteogenic effect of rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm) on human periodontal ligament cells (hPDLCs) was evaluated in osteogenic media.
32411618	11	94	theme	root	1633:1636	arg1	slices					1638:1643	The root slices	1629:1643	The root slices	1629:1643	The root slices were treated with rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm).
32411618	18	95	from	formation	2356:2364	arg1	vivo					2393:2396	vivo	2393:2396	vivo	2393:2396	Additionally, it promoted the formation of cementum-like tissue in vivo.
32411618	4	96	theme	electron	686:693	arg1	TEM					707:709	TEM	707:709	TEM	707:709	The physicochemical properties of mHA/CS scaffolds were examined by Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and Brunauer-Emmett-Teller (BET) analysis.
32411618	4	96	theme	electron	686:693	arg1	microscopy					695:704	transmission electron microscopy	673:704	transmission electron microscopy (TEM)	673:710	The physicochemical properties of mHA/CS scaffolds were examined by Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and Brunauer-Emmett-Teller (BET) analysis.
32411618	9	97	theme	baseline	1456:1463	arg1	hPDLCs					1344:1349	The hPDLCs	1340:1349	The hPDLCs treated either with osteogenic media or Dulbecco's modified Eagle's medium (DMEM) alone	1340:1437	The hPDLCs treated either with osteogenic media or Dulbecco's modified Eagle's medium (DMEM) alone were used as the baseline control.
32411618	9	97	theme	baseline	1456:1463	arg1	control					1465:1471	the baseline control	1452:1471	the baseline control	1452:1471	The hPDLCs treated either with osteogenic media or Dulbecco's modified Eagle's medium (DMEM) alone were used as the baseline control.
32411618	3	98	theme	study	389:393	arg1	aim					378:380	The aim	374:380	The aim of our study	374:393	The aim of our study is to evaluate the biological effects of mesoporous hydroxyapatite/chitosan (mHA/CS) loaded with recombinant human amelogenin (rhAm) on periodontal regeneration.
32411618	6	99	dep	mg/mL	1040:1044	arg1	mHA/CS					1046:1051	mHA/CS	1046:1051	mHA/CS	1046:1051	The antibacterial effect was tested on 1.5 mg/mL CS; 3 mg/mL mHA; 2.25 mg/mL mHA/CS; 4.5 mg/mL mHA/CS and 20 μg/mL rhAm.
32411618	10	100	theme	=	1528:1528	arg1	n					1526:1526	n = 6	1526:1530	n = 6	1526:1530	In the animal model, 4-week-old nude mice (BALB/c) (n = 6) implanted with root slices subcutaneously were used to observe the cementogenic effect in vivo.
32411618	10	100	theme	=	1528:1528	arg1	mice					1511:1514	4-week-old nude mice	1495:1514	4-week-old nude mice (BALB/c) (n = 6) implanted with root slices subcutaneously	1495:1573	In the animal model, 4-week-old nude mice (BALB/c) (n = 6) implanted with root slices subcutaneously were used to observe the cementogenic effect in vivo.
32411618	5	101	theme	antibacterial	842:854	arg1	effect					856:861	antibacterial effect	842:861	antibacterial effect	842:861	Then, the biological effects of the mHA/CS loaded with rhAm were evaluated, including antibacterial effect, controlled-release capacity, osteogenic and cementogenic effects in vitro and in vivo.
32411618	5	101	theme	antibacterial	842:854	arg1	capacity					883:890	controlled-release capacity	864:890	controlled-release capacity	864:890	Then, the biological effects of the mHA/CS loaded with rhAm were evaluated, including antibacterial effect, controlled-release capacity, osteogenic and cementogenic effects in vitro and in vivo.
32411618	5	101	theme	antibacterial	842:854	arg1	effects					921:927	osteogenic and cementogenic effects	893:927	osteogenic and cementogenic effects in vitro and in vivo	893:948	Then, the biological effects of the mHA/CS loaded with rhAm were evaluated, including antibacterial effect, controlled-release capacity, osteogenic and cementogenic effects in vitro and in vivo.
32411618	3	102	from	effects	425:431	arg1	regeneration					543:554	periodontal regeneration	531:554	periodontal regeneration	531:554	The aim of our study is to evaluate the biological effects of mesoporous hydroxyapatite/chitosan (mHA/CS) loaded with recombinant human amelogenin (rhAm) on periodontal regeneration.
32411618	6	103	theme	μg/mL	1060:1064	arg1	rhAm					1066:1069	20 μg/mL rhAm	1057:1069	20 μg/mL rhAm	1057:1069	The antibacterial effect was tested on 1.5 mg/mL CS; 3 mg/mL mHA; 2.25 mg/mL mHA/CS; 4.5 mg/mL mHA/CS and 20 μg/mL rhAm.
32411618	11	104	theme	20	1750:1751	arg1	μg/mL					1753:1757	μg/mL	1753:1757	μg/mL	1753:1757	The root slices were treated with rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm).
32411618	9	105	theme	modified	1402:1409	arg1	DMEM					1427:1430	DMEM	1427:1430	DMEM	1427:1430	The hPDLCs treated either with osteogenic media or Dulbecco's modified Eagle's medium (DMEM) alone were used as the baseline control.
32411618	9	105	theme	modified	1402:1409	arg1	medium					1419:1424	Dulbecco's modified Eagle's medium	1391:1424	Dulbecco's modified Eagle's medium (DMEM)	1391:1431	The hPDLCs treated either with osteogenic media or Dulbecco's modified Eagle's medium (DMEM) alone were used as the baseline control.
32411618	19	106	theme	periodontal	2486:2496	arg1	pathogens					2498:2506	periodontal pathogens	2486:2506	periodontal pathogens	2486:2506	Our findings suggest that mHA/CS loaded with 20 μg/mL rhAm could inhibit the growth of periodontal pathogens and promote the formation of bone and cementum-like tissue.
32411618	3	107	theme	human	504:508	arg1	rhAm					522:525	rhAm	522:525	rhAm	522:525	The aim of our study is to evaluate the biological effects of mesoporous hydroxyapatite/chitosan (mHA/CS) loaded with recombinant human amelogenin (rhAm) on periodontal regeneration.
32411618	3	107	theme	human	504:508	arg1	amelogenin					510:519	recombinant human amelogenin	492:519	recombinant human amelogenin (rhAm)	492:526	The aim of our study is to evaluate the biological effects of mesoporous hydroxyapatite/chitosan (mHA/CS) loaded with recombinant human amelogenin (rhAm) on periodontal regeneration.
32411618	3	108	theme	mesoporous	436:445	arg1	mHA/CS					472:477	mesoporous hydroxyapatite/chitosan (mHA/CS)	436:478	mesoporous hydroxyapatite/chitosan (mHA/CS) loaded with recombinant human amelogenin (rhAm)	436:526	The aim of our study is to evaluate the biological effects of mesoporous hydroxyapatite/chitosan (mHA/CS) loaded with recombinant human amelogenin (rhAm) on periodontal regeneration.
32411618	4	109	theme	scaffolds	598:606	arg1	properties					577:586	The physicochemical properties	557:586	The physicochemical properties of mHA/CS scaffolds	557:606	The physicochemical properties of mHA/CS scaffolds were examined by Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and Brunauer-Emmett-Teller (BET) analysis.
32411618	3	110	theme	periodontal	531:541	arg1	regeneration					543:554	periodontal regeneration	531:554	periodontal regeneration	531:554	The aim of our study is to evaluate the biological effects of mesoporous hydroxyapatite/chitosan (mHA/CS) loaded with recombinant human amelogenin (rhAm) on periodontal regeneration.
32411618	11	111	dep	mg/mL	1733:1737	arg1	mHA/CS					1739:1744	mHA/CS	1739:1744	mHA/CS	1739:1744	The root slices were treated with rhAm (20 μg/mL rhAm), mHA/CS (4.5 mg/mL mHA/CS), and mHA/CS-rhAm (4.5 mg/mL mHA/CS and 20 μg/mL rhAm).
32411618	17	112	theme	genes	2297:2301	arg1	activity					2241:2248	upregulated ALP activity	2225:2248	upregulated ALP activity	2225:2248	The mHA/CS loaded with 20 μg/mL rhAm upregulated ALP activity, the expression levels of osteogenesis-related genes and proteins in vitro.
32411618	17	112	theme	genes	2297:2301	arg1	proteins					2307:2314	proteins	2307:2314	proteins	2307:2314	The mHA/CS loaded with 20 μg/mL rhAm upregulated ALP activity, the expression levels of osteogenesis-related genes and proteins in vitro.
32411618	17	112	theme	genes	2297:2301	arg1	levels					2266:2271	the expression levels	2251:2271	the expression levels of osteogenesis-related genes	2251:2301	The mHA/CS loaded with 20 μg/mL rhAm upregulated ALP activity, the expression levels of osteogenesis-related genes and proteins in vitro.
32411618	15	113	theme	Porphyromonas	2115:2127	arg1	gingivalis					2129:2138	Porphyromonas gingivalis	2115:2138	Porphyromonas gingivalis	2115:2138	The scaffold exhibited antibacterial effects against Fusobacterium nucleatum and Porphyromonas gingivalis.
32411618	4	114	theme	transmission	673:684	arg1	TEM					707:709	TEM	707:709	TEM	707:709	The physicochemical properties of mHA/CS scaffolds were examined by Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and Brunauer-Emmett-Teller (BET) analysis.
32411618	4	114	theme	transmission	673:684	arg1	microscopy					695:704	transmission electron microscopy	673:704	transmission electron microscopy (TEM)	673:710	The physicochemical properties of mHA/CS scaffolds were examined by Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and Brunauer-Emmett-Teller (BET) analysis.
32540520	2	0	theme	SBP	353:355	arg1	level					344:348	the level	340:348	the level of SBP	340:355	The concentration of WPI was kept at a constant (3%), whereas the level of SBP was varied at 1, 1.5, and 3%.
32540520	4	1	dep	conditions	677:686	arg1	duration					727:734	reaction duration	718:734	reaction duration	718:734	The results demonstrated that the losses of the free sulfhydryl (-SH) and primary amine (-NH2) contents in WPI were less severe compared to those occurring in the dry-state at similar conditions (mass ratio, temperature, and reaction duration).
32540520	4	1	dep	conditions	677:686	arg1	temperature					701:711	temperature	701:711	temperature	701:711	The results demonstrated that the losses of the free sulfhydryl (-SH) and primary amine (-NH2) contents in WPI were less severe compared to those occurring in the dry-state at similar conditions (mass ratio, temperature, and reaction duration).
32540520	4	1	dep	conditions	677:686	arg1	conditions					677:686	similar conditions	669:686	similar conditions (mass ratio, temperature, and reaction duration)	669:735	The results demonstrated that the losses of the free sulfhydryl (-SH) and primary amine (-NH2) contents in WPI were less severe compared to those occurring in the dry-state at similar conditions (mass ratio, temperature, and reaction duration).
32540520	4	1	dep	conditions	677:686	arg1	ratio					694:698	mass ratio	689:698	mass ratio	689:698	The results demonstrated that the losses of the free sulfhydryl (-SH) and primary amine (-NH2) contents in WPI were less severe compared to those occurring in the dry-state at similar conditions (mass ratio, temperature, and reaction duration).
32540520	0	2	theme	isolate	72:78	arg1	systems					114:120	heated whey protein isolate (WPI) and sugar beet pectin (SBP) systems	52:120	heated whey protein isolate (WPI) and sugar beet pectin (SBP) systems	52:120	Chemical composition as an indicator for evaluating heated whey protein isolate (WPI) and sugar beet pectin (SBP) systems to stabilize O/W emulsions.
32540520	0	3	theme	O/W	135:137	arg1	emulsions					139:147	O/W emulsions	135:147	O/W emulsions	135:147	Chemical composition as an indicator for evaluating heated whey protein isolate (WPI) and sugar beet pectin (SBP) systems to stabilize O/W emulsions.
32540520	4	4	theme	reaction	718:725	arg1	duration					727:734	reaction duration	718:734	reaction duration	718:734	The results demonstrated that the losses of the free sulfhydryl (-SH) and primary amine (-NH2) contents in WPI were less severe compared to those occurring in the dry-state at similar conditions (mass ratio, temperature, and reaction duration).
32540520	4	4	theme	reaction	718:725	arg1	conditions					677:686	similar conditions	669:686	similar conditions (mass ratio, temperature, and reaction duration)	669:735	The results demonstrated that the losses of the free sulfhydryl (-SH) and primary amine (-NH2) contents in WPI were less severe compared to those occurring in the dry-state at similar conditions (mass ratio, temperature, and reaction duration).
32540520	4	5	theme	primary	567:573	arg1	-NH2					582:585	-NH2	582:585	-NH2	582:585	The results demonstrated that the losses of the free sulfhydryl (-SH) and primary amine (-NH2) contents in WPI were less severe compared to those occurring in the dry-state at similar conditions (mass ratio, temperature, and reaction duration).
32540520	4	5	theme	primary	567:573	arg1	amine					575:579	primary amine	567:579	primary amine (-NH2)	567:586	The results demonstrated that the losses of the free sulfhydryl (-SH) and primary amine (-NH2) contents in WPI were less severe compared to those occurring in the dry-state at similar conditions (mass ratio, temperature, and reaction duration).
32540520	5	6	theme	improved	836:843	arg1	emulsifiers					764:774	emulsifiers	764:774	emulsifiers in an O/W emulsion system	764:800	The mixtures were used as emulsifiers in an O/W emulsion system at pH 3.20 and 6.75 and showed an improved ability to stabilize the average size of the droplets than WPI alone at acidic pH. The mixtures at higher levels of SBP, ≥ 1.5%, however, adversely affected the emulsion stability at neutral pH.
32540520	5	6	theme	improved	836:843	arg1	mixtures					742:749	The mixtures	738:749	The mixtures	738:749	The mixtures were used as emulsifiers in an O/W emulsion system at pH 3.20 and 6.75 and showed an improved ability to stabilize the average size of the droplets than WPI alone at acidic pH. The mixtures at higher levels of SBP, ≥ 1.5%, however, adversely affected the emulsion stability at neutral pH.
32540520	5	6	theme	improved	836:843	arg1	ability					845:851	an improved ability to stabilize the average size of the droplets than WPI alone at acidic pH. The mixtures at higher levels of SBP, ≥ 1.5%, however, adversely affected the emulsion stability at neutral pH	833:1037	an improved ability to stabilize the average size of the droplets than WPI alone at acidic pH. The mixtures at higher levels of SBP, ≥ 1.5%, however, adversely affected the emulsion stability at neutral pH	833:1037	The mixtures were used as emulsifiers in an O/W emulsion system at pH 3.20 and 6.75 and showed an improved ability to stabilize the average size of the droplets than WPI alone at acidic pH. The mixtures at higher levels of SBP, ≥ 1.5%, however, adversely affected the emulsion stability at neutral pH.
32540520	5	7	theme	neutral	1028:1034	arg1	pH					1036:1037	neutral pH	1028:1037	neutral pH	1028:1037	The mixtures were used as emulsifiers in an O/W emulsion system at pH 3.20 and 6.75 and showed an improved ability to stabilize the average size of the droplets than WPI alone at acidic pH. The mixtures at higher levels of SBP, ≥ 1.5%, however, adversely affected the emulsion stability at neutral pH.
32540520	5	8	theme	higher	944:949	arg1	levels					951:956	higher levels	944:956	higher levels of SBP	944:963	The mixtures were used as emulsifiers in an O/W emulsion system at pH 3.20 and 6.75 and showed an improved ability to stabilize the average size of the droplets than WPI alone at acidic pH. The mixtures at higher levels of SBP, ≥ 1.5%, however, adversely affected the emulsion stability at neutral pH.
32540520	0	9	theme	sugar	90:94	arg1	beet					96:99	sugar beet	90:99	sugar beet pectin (SBP)	90:112	Chemical composition as an indicator for evaluating heated whey protein isolate (WPI) and sugar beet pectin (SBP) systems to stabilize O/W emulsions.
32540520	5	10	from	emulsifiers	764:774	arg1	system					795:800	an O/W emulsion system	779:800	an O/W emulsion system	779:800	The mixtures were used as emulsifiers in an O/W emulsion system at pH 3.20 and 6.75 and showed an improved ability to stabilize the average size of the droplets than WPI alone at acidic pH. The mixtures at higher levels of SBP, ≥ 1.5%, however, adversely affected the emulsion stability at neutral pH.
32540520	4	11	theme	amine	575:579	arg1	contents					588:595	the free sulfhydryl (-SH) and primary amine (-NH2) contents	537:595	the free sulfhydryl (-SH) and primary amine (-NH2) contents	537:595	The results demonstrated that the losses of the free sulfhydryl (-SH) and primary amine (-NH2) contents in WPI were less severe compared to those occurring in the dry-state at similar conditions (mass ratio, temperature, and reaction duration).
32540520	2	12	theme	WPI	299:301	arg1	concentration					282:294	The concentration	278:294	The concentration of WPI	278:301	The concentration of WPI was kept at a constant (3%), whereas the level of SBP was varied at 1, 1.5, and 3%.
32540520	3	13	theme	reaction	391:398	arg1	products					400:407	The reaction products	387:407	The reaction products	387:407	The reaction products were examined using the Ellman's reagent, ninhydrin assay, and gel electrophoresis.
32540520	0	14	theme	Chemical	0:7	arg1	composition					9:19	Chemical composition	0:19	Chemical composition as an indicator for evaluating heated whey protein isolate (WPI) and sugar beet pectin (SBP) systems	0:120	Chemical composition as an indicator for evaluating heated whey protein isolate (WPI) and sugar beet pectin (SBP) systems to stabilize O/W emulsions.
32540520	1	15	theme	heating	224:230	arg1	changes					166:172	changes	166:172	changes in the chemical composition of WPI	166:207	We investigated changes in the chemical composition of WPI as a result of heating (60 °C, 72 h) with SBP in solution (pH 6.75).
32540520	1	15	theme	heating	224:230	arg1	result					214:219	a result	212:219	a result of heating	212:230	We investigated changes in the chemical composition of WPI as a result of heating (60 °C, 72 h) with SBP in solution (pH 6.75).
32540520	4	16	theme	sulfhydryl	546:555	arg1	contents					588:595	the free sulfhydryl (-SH) and primary amine (-NH2) contents	537:595	the free sulfhydryl (-SH) and primary amine (-NH2) contents	537:595	The results demonstrated that the losses of the free sulfhydryl (-SH) and primary amine (-NH2) contents in WPI were less severe compared to those occurring in the dry-state at similar conditions (mass ratio, temperature, and reaction duration).
32540520	1	17	from	SBP	251:253	arg1	pH					268:269	pH 6.75	268:274	pH 6.75	268:274	We investigated changes in the chemical composition of WPI as a result of heating (60 °C, 72 h) with SBP in solution (pH 6.75).
32540520	1	17	from	SBP	251:253	arg1	solution					258:265	solution	258:265	solution (pH 6.75)	258:275	We investigated changes in the chemical composition of WPI as a result of heating (60 °C, 72 h) with SBP in solution (pH 6.75).
32540520	0	18	theme	pectin	101:106	arg1	systems					114:120	heated whey protein isolate (WPI) and sugar beet pectin (SBP) systems	52:120	heated whey protein isolate (WPI) and sugar beet pectin (SBP) systems	52:120	Chemical composition as an indicator for evaluating heated whey protein isolate (WPI) and sugar beet pectin (SBP) systems to stabilize O/W emulsions.
32540520	5	19	theme	emulsion	1006:1013	arg1	stability					1015:1023	the emulsion stability	1002:1023	the emulsion stability at neutral pH	1002:1037	The mixtures were used as emulsifiers in an O/W emulsion system at pH 3.20 and 6.75 and showed an improved ability to stabilize the average size of the droplets than WPI alone at acidic pH. The mixtures at higher levels of SBP, ≥ 1.5%, however, adversely affected the emulsion stability at neutral pH.
32540520	5	20	theme	average	870:876	arg1	size					878:881	the average size	866:881	the average size of the droplets than WPI alone	866:912	The mixtures were used as emulsifiers in an O/W emulsion system at pH 3.20 and 6.75 and showed an improved ability to stabilize the average size of the droplets than WPI alone at acidic pH. The mixtures at higher levels of SBP, ≥ 1.5%, however, adversely affected the emulsion stability at neutral pH.
32540520	0	21	theme	beet	96:99	arg1	SBP					109:111	SBP	109:111	SBP	109:111	Chemical composition as an indicator for evaluating heated whey protein isolate (WPI) and sugar beet pectin (SBP) systems to stabilize O/W emulsions.
32540520	0	21	theme	beet	96:99	arg1	pectin					101:106	sugar beet pectin	90:106	sugar beet pectin (SBP)	90:112	Chemical composition as an indicator for evaluating heated whey protein isolate (WPI) and sugar beet pectin (SBP) systems to stabilize O/W emulsions.
32540520	5	22	dep	pH.	924:926	arg1	affected					993:1000	affected	993:1000	affected the emulsion stability at neutral pH	993:1037	The mixtures were used as emulsifiers in an O/W emulsion system at pH 3.20 and 6.75 and showed an improved ability to stabilize the average size of the droplets than WPI alone at acidic pH. The mixtures at higher levels of SBP, ≥ 1.5%, however, adversely affected the emulsion stability at neutral pH.
32540520	3	23	theme	ninhydrin	451:459	arg1	assay					461:465	ninhydrin assay	451:465	ninhydrin assay	451:465	The reaction products were examined using the Ellman's reagent, ninhydrin assay, and gel electrophoresis.
32540520	5	24	theme	O/W	782:784	arg1	system					795:800	an O/W emulsion system	779:800	an O/W emulsion system	779:800	The mixtures were used as emulsifiers in an O/W emulsion system at pH 3.20 and 6.75 and showed an improved ability to stabilize the average size of the droplets than WPI alone at acidic pH. The mixtures at higher levels of SBP, ≥ 1.5%, however, adversely affected the emulsion stability at neutral pH.
32540520	0	25	theme	heated	52:57	arg1	systems					114:120	heated whey protein isolate (WPI) and sugar beet pectin (SBP) systems	52:120	heated whey protein isolate (WPI) and sugar beet pectin (SBP) systems	52:120	Chemical composition as an indicator for evaluating heated whey protein isolate (WPI) and sugar beet pectin (SBP) systems to stabilize O/W emulsions.
32540520	5	26	theme	droplets	890:897	arg1	size					878:881	the average size	866:881	the average size of the droplets than WPI alone	866:912	The mixtures were used as emulsifiers in an O/W emulsion system at pH 3.20 and 6.75 and showed an improved ability to stabilize the average size of the droplets than WPI alone at acidic pH. The mixtures at higher levels of SBP, ≥ 1.5%, however, adversely affected the emulsion stability at neutral pH.
32540520	5	27	theme	emulsion	786:793	arg1	system					795:800	an O/W emulsion system	779:800	an O/W emulsion system	779:800	The mixtures were used as emulsifiers in an O/W emulsion system at pH 3.20 and 6.75 and showed an improved ability to stabilize the average size of the droplets than WPI alone at acidic pH. The mixtures at higher levels of SBP, ≥ 1.5%, however, adversely affected the emulsion stability at neutral pH.
32540520	5	28	from	levels	951:956	arg1	mixtures					932:939	The mixtures	928:939	The mixtures at higher levels of SBP	928:963	The mixtures were used as emulsifiers in an O/W emulsion system at pH 3.20 and 6.75 and showed an improved ability to stabilize the average size of the droplets than WPI alone at acidic pH. The mixtures at higher levels of SBP, ≥ 1.5%, however, adversely affected the emulsion stability at neutral pH.
32540520	4	29	theme	similar	669:675	arg1	duration					727:734	reaction duration	718:734	reaction duration	718:734	The results demonstrated that the losses of the free sulfhydryl (-SH) and primary amine (-NH2) contents in WPI were less severe compared to those occurring in the dry-state at similar conditions (mass ratio, temperature, and reaction duration).
32540520	4	29	theme	similar	669:675	arg1	temperature					701:711	temperature	701:711	temperature	701:711	The results demonstrated that the losses of the free sulfhydryl (-SH) and primary amine (-NH2) contents in WPI were less severe compared to those occurring in the dry-state at similar conditions (mass ratio, temperature, and reaction duration).
32540520	4	29	theme	similar	669:675	arg1	conditions					677:686	similar conditions	669:686	similar conditions (mass ratio, temperature, and reaction duration)	669:735	The results demonstrated that the losses of the free sulfhydryl (-SH) and primary amine (-NH2) contents in WPI were less severe compared to those occurring in the dry-state at similar conditions (mass ratio, temperature, and reaction duration).
32540520	4	29	theme	similar	669:675	arg1	ratio					694:698	mass ratio	689:698	mass ratio	689:698	The results demonstrated that the losses of the free sulfhydryl (-SH) and primary amine (-NH2) contents in WPI were less severe compared to those occurring in the dry-state at similar conditions (mass ratio, temperature, and reaction duration).
32540520	5	30	from	pH	1036:1037	arg1	stability					1015:1023	the emulsion stability	1002:1023	the emulsion stability at neutral pH	1002:1037	The mixtures were used as emulsifiers in an O/W emulsion system at pH 3.20 and 6.75 and showed an improved ability to stabilize the average size of the droplets than WPI alone at acidic pH. The mixtures at higher levels of SBP, ≥ 1.5%, however, adversely affected the emulsion stability at neutral pH.
32540520	4	31	theme	mass	689:692	arg1	conditions					677:686	similar conditions	669:686	similar conditions (mass ratio, temperature, and reaction duration)	669:735	The results demonstrated that the losses of the free sulfhydryl (-SH) and primary amine (-NH2) contents in WPI were less severe compared to those occurring in the dry-state at similar conditions (mass ratio, temperature, and reaction duration).
32540520	4	31	theme	mass	689:692	arg1	ratio					694:698	mass ratio	689:698	mass ratio	689:698	The results demonstrated that the losses of the free sulfhydryl (-SH) and primary amine (-NH2) contents in WPI were less severe compared to those occurring in the dry-state at similar conditions (mass ratio, temperature, and reaction duration).
32540520	1	32	from	changes	166:172	arg1	composition					190:200	the chemical composition	177:200	the chemical composition of WPI	177:207	We investigated changes in the chemical composition of WPI as a result of heating (60 °C, 72 h) with SBP in solution (pH 6.75).
32540520	4	33	theme	free	541:544	arg1	-SH					558:560	-SH	558:560	-SH	558:560	The results demonstrated that the losses of the free sulfhydryl (-SH) and primary amine (-NH2) contents in WPI were less severe compared to those occurring in the dry-state at similar conditions (mass ratio, temperature, and reaction duration).
32540520	4	33	theme	free	541:544	arg1	sulfhydryl					546:555	the free sulfhydryl	537:555	the free sulfhydryl (-SH)	537:561	The results demonstrated that the losses of the free sulfhydryl (-SH) and primary amine (-NH2) contents in WPI were less severe compared to those occurring in the dry-state at similar conditions (mass ratio, temperature, and reaction duration).
32540520	1	34	theme	chemical	181:188	arg1	composition					190:200	the chemical composition	177:200	the chemical composition of WPI	177:207	We investigated changes in the chemical composition of WPI as a result of heating (60 °C, 72 h) with SBP in solution (pH 6.75).
32540520	3	35	theme	gel	472:474	arg1	electrophoresis					476:490	gel electrophoresis	472:490	gel electrophoresis	472:490	The reaction products were examined using the Ellman's reagent, ninhydrin assay, and gel electrophoresis.
32540520	5	36	used	used	756:759	arg2	mixtures					742:749	The mixtures	738:749	The mixtures	738:749	The mixtures were used as emulsifiers in an O/W emulsion system at pH 3.20 and 6.75 and showed an improved ability to stabilize the average size of the droplets than WPI alone at acidic pH. The mixtures at higher levels of SBP, ≥ 1.5%, however, adversely affected the emulsion stability at neutral pH.
32540520	5	36	used	used	756:759	arg2	ability					845:851	an improved ability to stabilize the average size of the droplets than WPI alone at acidic pH. The mixtures at higher levels of SBP, ≥ 1.5%, however, adversely affected the emulsion stability at neutral pH	833:1037	an improved ability to stabilize the average size of the droplets than WPI alone at acidic pH. The mixtures at higher levels of SBP, ≥ 1.5%, however, adversely affected the emulsion stability at neutral pH	833:1037	The mixtures were used as emulsifiers in an O/W emulsion system at pH 3.20 and 6.75 and showed an improved ability to stabilize the average size of the droplets than WPI alone at acidic pH. The mixtures at higher levels of SBP, ≥ 1.5%, however, adversely affected the emulsion stability at neutral pH.
32540520	5	36	used	used	756:759	arg2	emulsifiers					764:774	emulsifiers	764:774	emulsifiers in an O/W emulsion system	764:800	The mixtures were used as emulsifiers in an O/W emulsion system at pH 3.20 and 6.75 and showed an improved ability to stabilize the average size of the droplets than WPI alone at acidic pH. The mixtures at higher levels of SBP, ≥ 1.5%, however, adversely affected the emulsion stability at neutral pH.
32540520	0	37	theme	protein	64:70	arg1	isolate					72:78	whey protein isolate	59:78	whey protein isolate (WPI)	59:84	Chemical composition as an indicator for evaluating heated whey protein isolate (WPI) and sugar beet pectin (SBP) systems to stabilize O/W emulsions.
32540520	0	37	theme	protein	64:70	arg1	WPI					81:83	WPI	81:83	WPI	81:83	Chemical composition as an indicator for evaluating heated whey protein isolate (WPI) and sugar beet pectin (SBP) systems to stabilize O/W emulsions.
32540520	1	38	dep	investigated	153:164	arg1	72 h					240:243	72 h	240:243	72 h	240:243	We investigated changes in the chemical composition of WPI as a result of heating (60 °C, 72 h) with SBP in solution (pH 6.75).
32540520	4	39	from	losses	527:532	arg1	WPI					600:602	WPI	600:602	WPI	600:602	The results demonstrated that the losses of the free sulfhydryl (-SH) and primary amine (-NH2) contents in WPI were less severe compared to those occurring in the dry-state at similar conditions (mass ratio, temperature, and reaction duration).
32540520	5	40	theme	SBP	961:963	arg1	levels					951:956	higher levels	944:956	higher levels of SBP	944:963	The mixtures were used as emulsifiers in an O/W emulsion system at pH 3.20 and 6.75 and showed an improved ability to stabilize the average size of the droplets than WPI alone at acidic pH. The mixtures at higher levels of SBP, ≥ 1.5%, however, adversely affected the emulsion stability at neutral pH.
32540520	0	41	theme	whey	59:62	arg1	isolate					72:78	whey protein isolate	59:78	whey protein isolate (WPI)	59:84	Chemical composition as an indicator for evaluating heated whey protein isolate (WPI) and sugar beet pectin (SBP) systems to stabilize O/W emulsions.
32540520	0	41	theme	whey	59:62	arg1	WPI					81:83	WPI	81:83	WPI	81:83	Chemical composition as an indicator for evaluating heated whey protein isolate (WPI) and sugar beet pectin (SBP) systems to stabilize O/W emulsions.
32540520	4	42	theme	contents	588:595	arg1	losses					527:532	the losses	523:532	the losses of the free sulfhydryl (-SH) and primary amine (-NH2) contents in WPI	523:602	The results demonstrated that the losses of the free sulfhydryl (-SH) and primary amine (-NH2) contents in WPI were less severe compared to those occurring in the dry-state at similar conditions (mass ratio, temperature, and reaction duration).
32540520	4	42	theme	contents	588:595	arg1	severe					614:619	severe	614:619	severe	614:619	The results demonstrated that the losses of the free sulfhydryl (-SH) and primary amine (-NH2) contents in WPI were less severe compared to those occurring in the dry-state at similar conditions (mass ratio, temperature, and reaction duration).
32540520	1	43	theme	WPI	205:207	arg1	composition					190:200	the chemical composition	177:200	the chemical composition of WPI	177:207	We investigated changes in the chemical composition of WPI as a result of heating (60 °C, 72 h) with SBP in solution (pH 6.75).
32279815	4	0	theme	resection	556:564	arg1	cavity					566:571	the brain resection cavity	546:571	the brain resection cavity	546:571	Here, we investigated the impact of scaffold degradation rate on NSC persistence in the brain resection cavity.
32279815	13	1	theme	slow	1726:1729	arg1	scaffolds					1741:1749	Fast and slow degrading scaffolds	1717:1749	Fast and slow degrading scaffolds	1717:1749	Fast and slow degrading scaffolds enhanced NSC implantation efficiency 2.87 and 3.08-fold over DI, respectively.
32279815	4	2	from	impact	488:493	arg1	persistence					531:541	NSC persistence	527:541	NSC persistence in the brain resection cavity	527:571	Here, we investigated the impact of scaffold degradation rate on NSC persistence in the brain resection cavity.
32279815	12	3	theme	current	1667:1673	arg1	approach					1684:1691	the current clinical approach	1663:1691	the current clinical approach to NSC therapy for GBM	1663:1714	NSC loaded scaffolds were compared to a direct injection (DI) of NSCs in suspension, which is the current clinical approach to NSC therapy for GBM.
32279815	12	3	theme	current	1667:1673	arg1	suspension					1642:1651	suspension	1642:1651	suspension	1642:1651	NSC loaded scaffolds were compared to a direct injection (DI) of NSCs in suspension, which is the current clinical approach to NSC therapy for GBM.
32279815	9	4	theme	same	1176:1179	arg1	TCPS					1233:1236	tissue culture treated polystyrene (TCPS)	1197:1237	tissue culture treated polystyrene (TCPS)	1197:1237	Therapeutic NSCs secreting tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) had the same TRAIL output as tissue culture treated polystyrene (TCPS) when seeded on both scaffolds.
32279815	9	4	theme	same	1176:1179	arg1	output					1187:1192	the same TRAIL output	1172:1192	the same TRAIL output	1172:1192	Therapeutic NSCs secreting tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) had the same TRAIL output as tissue culture treated polystyrene (TCPS) when seeded on both scaffolds.
32279815	2	5	theme	engineered	249:258	arg1	NSCs					260:263	genetically engineered NSCs	237:263	genetically engineered NSCs that secrete tumoricidal agents	237:295	This therapy employs genetically engineered NSCs that secrete tumoricidal agents to seek out and kill tumor foci remaining after GBM surgical resection.
32279815	6	6	theme	slow	853:856	arg1	scaffolds					868:876	slow degrading scaffolds	853:876	slow degrading scaffolds	853:876	In vitro, fast degrading scaffolds were fully degraded by one week, whereas slow degrading scaffolds had a half-life of >56 days.
32279815	8	7	theme	different	978:986	arg1	lines					992:996	Two different NSC lines	974:996	Two different NSC lines	974:996	Two different NSC lines readily adhered to and remained viable on Ace-DEX gelatin scaffolds, in vitro.
32279815	5	8	theme	electrospun	621:631	arg1	scaffolds					633:641	Composite acetalated dextran (Ace-DEX) gelatin electrospun scaffolds	574:641	Composite acetalated dextran (Ace-DEX) gelatin electrospun scaffolds	574:641	Composite acetalated dextran (Ace-DEX) gelatin electrospun scaffolds were fabricated with two distinct degradation profiles created by changing the ratio of cyclic to acyclic acetal coverage of Ace-DEX.
32279815	9	9	theme	tissue	1197:1202	arg1	TCPS					1233:1236	tissue culture treated polystyrene (TCPS)	1197:1237	tissue culture treated polystyrene (TCPS)	1197:1237	Therapeutic NSCs secreting tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) had the same TRAIL output as tissue culture treated polystyrene (TCPS) when seeded on both scaffolds.
32279815	9	9	theme	tissue	1197:1202	arg1	output					1187:1192	the same TRAIL output	1172:1192	the same TRAIL output	1172:1192	Therapeutic NSCs secreting tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) had the same TRAIL output as tissue culture treated polystyrene (TCPS) when seeded on both scaffolds.
32279815	1	10	theme	emerging	169:176	arg1	cells					149:153	Tumoricidal neural stem cells	125:153	Tumoricidal neural stem cells (NSCs)	125:160	Tumoricidal neural stem cells (NSCs) are an emerging therapy to combat glioblastoma (GBM).
32279815	1	10	theme	emerging	169:176	arg1	therapy					178:184	an emerging therapy	166:184	an emerging therapy to combat glioblastoma (GBM)	166:213	Tumoricidal neural stem cells (NSCs) are an emerging therapy to combat glioblastoma (GBM).
32279815	5	11	dep	coverage	756:763	arg1	to					738:739	to	738:739	to	738:739	Composite acetalated dextran (Ace-DEX) gelatin electrospun scaffolds were fabricated with two distinct degradation profiles created by changing the ratio of cyclic to acyclic acetal coverage of Ace-DEX.
32279815	9	12	theme	treated	1212:1218	arg1	TCPS					1233:1236	tissue culture treated polystyrene (TCPS)	1197:1237	tissue culture treated polystyrene (TCPS)	1197:1237	Therapeutic NSCs secreting tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) had the same TRAIL output as tissue culture treated polystyrene (TCPS) when seeded on both scaffolds.
32279815	9	12	theme	treated	1212:1218	arg1	output					1187:1192	the same TRAIL output	1172:1192	the same TRAIL output	1172:1192	Therapeutic NSCs secreting tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) had the same TRAIL output as tissue culture treated polystyrene (TCPS) when seeded on both scaffolds.
32279815	2	13	theme	tumoricidal	278:288	arg1	agents					290:295	tumoricidal agents	278:295	tumoricidal agents	278:295	This therapy employs genetically engineered NSCs that secrete tumoricidal agents to seek out and kill tumor foci remaining after GBM surgical resection.
32279815	0	14	from	persistence	66:76	arg1	cavity					117:122	the glioblastoma surgical resection cavity	81:122	the glioblastoma surgical resection cavity	81:122	Impact of composite scaffold degradation rate on neural stem cell persistence in the glioblastoma surgical resection cavity.
32279815	12	15	from	suspension	1642:1651	arg1	DI					1627:1628	DI	1627:1628	DI	1627:1628	NSC loaded scaffolds were compared to a direct injection (DI) of NSCs in suspension, which is the current clinical approach to NSC therapy for GBM.
32279815	12	15	from	suspension	1642:1651	arg1	injection					1616:1624	a direct injection	1607:1624	a direct injection (DI) of NSCs in suspension, which is the current clinical approach to NSC therapy for GBM	1607:1714	NSC loaded scaffolds were compared to a direct injection (DI) of NSCs in suspension, which is the current clinical approach to NSC therapy for GBM.
32279815	4	16	from	persistence	531:541	arg1	cavity					566:571	the brain resection cavity	546:571	the brain resection cavity	546:571	Here, we investigated the impact of scaffold degradation rate on NSC persistence in the brain resection cavity.
32279815	12	17	from	NSCs	1634:1637	arg1	approach					1684:1691	the current clinical approach	1663:1691	the current clinical approach to NSC therapy for GBM	1663:1714	NSC loaded scaffolds were compared to a direct injection (DI) of NSCs in suspension, which is the current clinical approach to NSC therapy for GBM.
32279815	12	17	from	NSCs	1634:1637	arg1	suspension					1642:1651	suspension	1642:1651	suspension	1642:1651	NSC loaded scaffolds were compared to a direct injection (DI) of NSCs in suspension, which is the current clinical approach to NSC therapy for GBM.
32279815	5	18	theme	distinct	668:675	arg1	profiles					689:696	two distinct degradation profiles	664:696	two distinct degradation profiles created by changing the ratio of cyclic to acyclic acetal coverage of Ace-DEX	664:774	Composite acetalated dextran (Ace-DEX) gelatin electrospun scaffolds were fabricated with two distinct degradation profiles created by changing the ratio of cyclic to acyclic acetal coverage of Ace-DEX.
32279815	12	19	theme	NSCs	1634:1637	arg1	DI					1627:1628	DI	1627:1628	DI	1627:1628	NSC loaded scaffolds were compared to a direct injection (DI) of NSCs in suspension, which is the current clinical approach to NSC therapy for GBM.
32279815	12	19	theme	NSCs	1634:1637	arg1	injection					1616:1624	a direct injection	1607:1624	a direct injection (DI) of NSCs in suspension, which is the current clinical approach to NSC therapy for GBM	1607:1714	NSC loaded scaffolds were compared to a direct injection (DI) of NSCs in suspension, which is the current clinical approach to NSC therapy for GBM.
32279815	6	20	theme	degrading	792:800	arg1	scaffolds					802:810	fast degrading scaffolds	787:810	fast degrading scaffolds	787:810	In vitro, fast degrading scaffolds were fully degraded by one week, whereas slow degrading scaffolds had a half-life of >56 days.
32279815	1	21	theme	neural	137:142	arg1	cells					149:153	Tumoricidal neural stem cells	125:153	Tumoricidal neural stem cells (NSCs)	125:160	Tumoricidal neural stem cells (NSCs) are an emerging therapy to combat glioblastoma (GBM).
32279815	1	21	theme	neural	137:142	arg1	therapy					178:184	an emerging therapy	166:184	an emerging therapy to combat glioblastoma (GBM)	166:213	Tumoricidal neural stem cells (NSCs) are an emerging therapy to combat glioblastoma (GBM).
32279815	1	21	theme	neural	137:142	arg1	NSCs					156:159	NSCs	156:159	NSCs	156:159	Tumoricidal neural stem cells (NSCs) are an emerging therapy to combat glioblastoma (GBM).
32279815	16	22	theme	NSC	2209:2211	arg1	therapy					2213:2219	tumoricidal NSC therapy	2197:2219	tumoricidal NSC therapy for GBM	2197:2227	Overall, these results highlight the benefit of utilizing a scaffold for application of tumoricidal NSC therapy for GBM.
32279815	5	23	theme	acetal	749:754	arg1	coverage					756:763	cyclic to acyclic acetal coverage	731:763	cyclic to acyclic acetal coverage of Ace-DEX	731:774	Composite acetalated dextran (Ace-DEX) gelatin electrospun scaffolds were fabricated with two distinct degradation profiles created by changing the ratio of cyclic to acyclic acetal coverage of Ace-DEX.
32279815	11	24	theme	resection	1470:1478	arg1	cavity					1480:1485	a surgical resection cavity	1459:1485	a surgical resection cavity	1459:1485	Firefly luciferase expressing NSCs were seeded on scaffolds, implanted in a surgical resection cavity and their persistence in the brain was monitored by bioluminescent imaging (BLI).
32279815	5	25	theme	Composite	574:582	arg1	Ace-DEX					604:610	Ace-DEX	604:610	Ace-DEX	604:610	Composite acetalated dextran (Ace-DEX) gelatin electrospun scaffolds were fabricated with two distinct degradation profiles created by changing the ratio of cyclic to acyclic acetal coverage of Ace-DEX.
32279815	5	25	theme	Composite	574:582	arg1	dextran					595:601	Composite acetalated dextran	574:601	Composite acetalated dextran (Ace-DEX) gelatin electrospun scaffolds	574:641	Composite acetalated dextran (Ace-DEX) gelatin electrospun scaffolds were fabricated with two distinct degradation profiles created by changing the ratio of cyclic to acyclic acetal coverage of Ace-DEX.
32279815	11	26	from	persistence	1497:1507	arg1	brain					1516:1520	the brain	1512:1520	the brain	1512:1520	Firefly luciferase expressing NSCs were seeded on scaffolds, implanted in a surgical resection cavity and their persistence in the brain was monitored by bioluminescent imaging (BLI).
32279815	0	27	from	Impact	0:5	arg1	persistence					66:76	neural stem cell persistence	49:76	neural stem cell persistence in the glioblastoma surgical resection cavity	49:122	Impact of composite scaffold degradation rate on neural stem cell persistence in the glioblastoma surgical resection cavity.
32279815	6	28	theme	fast	787:790	arg1	scaffolds					802:810	fast degrading scaffolds	787:810	fast degrading scaffolds	787:810	In vitro, fast degrading scaffolds were fully degraded by one week, whereas slow degrading scaffolds had a half-life of >56 days.
32279815	5	29	theme	dextran	595:601	arg1	scaffolds					633:641	Composite acetalated dextran (Ace-DEX) gelatin electrospun scaffolds	574:641	Composite acetalated dextran (Ace-DEX) gelatin electrospun scaffolds	574:641	Composite acetalated dextran (Ace-DEX) gelatin electrospun scaffolds were fabricated with two distinct degradation profiles created by changing the ratio of cyclic to acyclic acetal coverage of Ace-DEX.
32279815	10	30	theme	secreted	1283:1290	arg1	TRAIL					1292:1296	secreted TRAIL	1283:1296	secreted TRAIL	1283:1296	Furthermore, secreted TRAIL was found to be highly potent against the human derived GBM cell line, GBM8, in vitro.
32279815	11	31	theme	luciferase	1393:1402	arg1	NSCs					1415:1418	Firefly luciferase expressing NSCs	1385:1418	Firefly luciferase expressing NSCs	1385:1418	Firefly luciferase expressing NSCs were seeded on scaffolds, implanted in a surgical resection cavity and their persistence in the brain was monitored by bioluminescent imaging (BLI).
32279815	0	32	theme	surgical	98:105	arg1	cavity					117:122	the glioblastoma surgical resection cavity	81:122	the glioblastoma surgical resection cavity	81:122	Impact of composite scaffold degradation rate on neural stem cell persistence in the glioblastoma surgical resection cavity.
32279815	0	33	theme	rate	41:44	arg1	Impact					0:5	Impact	0:5	Impact of composite scaffold degradation rate on neural stem cell persistence in the glioblastoma surgical resection cavity.	0:123	Impact of composite scaffold degradation rate on neural stem cell persistence in the glioblastoma surgical resection cavity.
32279815	12	34	theme	direct	1609:1614	arg1	DI					1627:1628	DI	1627:1628	DI	1627:1628	NSC loaded scaffolds were compared to a direct injection (DI) of NSCs in suspension, which is the current clinical approach to NSC therapy for GBM.
32279815	12	34	theme	direct	1609:1614	arg1	injection					1616:1624	a direct injection	1607:1624	a direct injection (DI) of NSCs in suspension, which is the current clinical approach to NSC therapy for GBM	1607:1714	NSC loaded scaffolds were compared to a direct injection (DI) of NSCs in suspension, which is the current clinical approach to NSC therapy for GBM.
32279815	0	35	theme	cell	61:64	arg1	persistence					66:76	neural stem cell persistence	49:76	neural stem cell persistence in the glioblastoma surgical resection cavity	49:122	Impact of composite scaffold degradation rate on neural stem cell persistence in the glioblastoma surgical resection cavity.
32279815	8	36	theme	Ace-DEX	1040:1046	arg1	scaffolds					1056:1064	Ace-DEX gelatin scaffolds	1040:1064	Ace-DEX gelatin scaffolds	1040:1064	Two different NSC lines readily adhered to and remained viable on Ace-DEX gelatin scaffolds, in vitro.
32279815	12	37	theme	loaded	1573:1578	arg1	scaffolds					1580:1588	NSC loaded scaffolds	1569:1588	NSC loaded scaffolds	1569:1588	NSC loaded scaffolds were compared to a direct injection (DI) of NSCs in suspension, which is the current clinical approach to NSC therapy for GBM.
32279815	4	38	theme	NSC	527:529	arg1	persistence					531:541	NSC persistence	527:541	NSC persistence in the brain resection cavity	527:571	Here, we investigated the impact of scaffold degradation rate on NSC persistence in the brain resection cavity.
32279815	15	39	theme	long-term	1945:1953	arg1	survival					1955:1962	long-term survival	1945:1962	long-term survival	1945:1962	However, persistence and long-term survival of NSCs was significantly greater for both scaffolds compared to DI, with scaffold implanted NSCs still detected by BLI at day 120 in most mice.
32279815	9	40	theme	Therapeutic	1077:1087	arg1	NSCs					1089:1092	Therapeutic NSCs	1077:1092	Therapeutic NSCs secreting tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)	1077:1166	Therapeutic NSCs secreting tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) had the same TRAIL output as tissue culture treated polystyrene (TCPS) when seeded on both scaffolds.
32279815	3	41	theme	resection	444:452	arg1	cavity					454:459	the resection cavity	440:459	the resection cavity	440:459	Biomaterial scaffolds have previously been utilized to deliver NSCs to the resection cavity.
32279815	0	42	theme	composite	10:18	arg1	rate					41:44	composite scaffold degradation rate	10:44	composite scaffold degradation rate	10:44	Impact of composite scaffold degradation rate on neural stem cell persistence in the glioblastoma surgical resection cavity.
32279815	15	43	theme	NSCs	1967:1970	arg1	persistence					1929:1939	persistence	1929:1939	persistence	1929:1939	However, persistence and long-term survival of NSCs was significantly greater for both scaffolds compared to DI, with scaffold implanted NSCs still detected by BLI at day 120 in most mice.
32279815	15	43	theme	NSCs	1967:1970	arg1	survival					1955:1962	long-term survival	1945:1962	long-term survival	1945:1962	However, persistence and long-term survival of NSCs was significantly greater for both scaffolds compared to DI, with scaffold implanted NSCs still detected by BLI at day 120 in most mice.
32279815	13	44	theme	NSC	1760:1762	arg1	efficiency					1777:1786	NSC implantation efficiency 2.87	1760:1791	NSC implantation efficiency 2.87	1760:1791	Fast and slow degrading scaffolds enhanced NSC implantation efficiency 2.87 and 3.08-fold over DI, respectively.
32279815	0	45	theme	degradation	29:39	arg1	rate					41:44	composite scaffold degradation rate	10:44	composite scaffold degradation rate	10:44	Impact of composite scaffold degradation rate on neural stem cell persistence in the glioblastoma surgical resection cavity.
32279815	15	46	located	detected	2068:2075	arg2	survival					1955:1962	long-term survival	1945:1962	long-term survival	1945:1962	However, persistence and long-term survival of NSCs was significantly greater for both scaffolds compared to DI, with scaffold implanted NSCs still detected by BLI at day 120 in most mice.
32279815	15	46	located	detected	2068:2075	arg1	day					2087:2089	day 120	2087:2093	day 120	2087:2093	However, persistence and long-term survival of NSCs was significantly greater for both scaffolds compared to DI, with scaffold implanted NSCs still detected by BLI at day 120 in most mice.
32279815	15	46	located	detected	2068:2075	arg1	mice					2103:2106	most mice	2098:2106	most mice	2098:2106	However, persistence and long-term survival of NSCs was significantly greater for both scaffolds compared to DI, with scaffold implanted NSCs still detected by BLI at day 120 in most mice.
32279815	15	46	located	detected	2068:2075	arg2	persistence					1929:1939	persistence	1929:1939	persistence	1929:1939	However, persistence and long-term survival of NSCs was significantly greater for both scaffolds compared to DI, with scaffold implanted NSCs still detected by BLI at day 120 in most mice.
32279815	14	47	theme	impact	1896:1901	arg1	persistence					1907:1917	impact NSC persistence	1896:1917	impact NSC persistence	1896:1917	Interestingly, scaffold degradation profile did not significantly impact NSC persistence.
32279815	4	48	theme	scaffold	498:505	arg1	rate					519:522	scaffold degradation rate	498:522	scaffold degradation rate	498:522	Here, we investigated the impact of scaffold degradation rate on NSC persistence in the brain resection cavity.
32279815	0	49	theme	stem	56:59	arg1	persistence					66:76	neural stem cell persistence	49:76	neural stem cell persistence in the glioblastoma surgical resection cavity	49:122	Impact of composite scaffold degradation rate on neural stem cell persistence in the glioblastoma surgical resection cavity.
32279815	2	50	theme	tumor	318:322	arg1	foci					324:327	tumor foci	318:327	tumor foci remaining after GBM surgical resection	318:366	This therapy employs genetically engineered NSCs that secrete tumoricidal agents to seek out and kill tumor foci remaining after GBM surgical resection.
32279815	15	51	theme	scaffold	2038:2045	arg1	NSCs					2057:2060	scaffold implanted NSCs	2038:2060	scaffold implanted NSCs	2038:2060	However, persistence and long-term survival of NSCs was significantly greater for both scaffolds compared to DI, with scaffold implanted NSCs still detected by BLI at day 120 in most mice.
32279815	4	52	theme	rate	519:522	arg1	impact					488:493	the impact	484:493	the impact of scaffold degradation rate on NSC persistence in the brain resection cavity	484:571	Here, we investigated the impact of scaffold degradation rate on NSC persistence in the brain resection cavity.
32279815	9	53	theme	apoptosis-inducing	1134:1151	arg1	TRAIL					1161:1165	TRAIL	1161:1165	TRAIL	1161:1165	Therapeutic NSCs secreting tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) had the same TRAIL output as tissue culture treated polystyrene (TCPS) when seeded on both scaffolds.
32279815	9	53	theme	apoptosis-inducing	1134:1151	arg1	ligand					1153:1158	factor-related apoptosis-inducing ligand	1119:1158	tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)	1104:1166	Therapeutic NSCs secreting tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) had the same TRAIL output as tissue culture treated polystyrene (TCPS) when seeded on both scaffolds.
32279815	11	54	theme	bioluminescent	1539:1552	arg1	BLI					1563:1565	BLI	1563:1565	BLI	1563:1565	Firefly luciferase expressing NSCs were seeded on scaffolds, implanted in a surgical resection cavity and their persistence in the brain was monitored by bioluminescent imaging (BLI).
32279815	11	54	theme	bioluminescent	1539:1552	arg1	imaging					1554:1560	bioluminescent imaging	1539:1560	bioluminescent imaging (BLI)	1539:1566	Firefly luciferase expressing NSCs were seeded on scaffolds, implanted in a surgical resection cavity and their persistence in the brain was monitored by bioluminescent imaging (BLI).
32279815	2	55	theme	GBM	345:347	arg1	resection					358:366	GBM surgical resection	345:366	GBM surgical resection	345:366	This therapy employs genetically engineered NSCs that secrete tumoricidal agents to seek out and kill tumor foci remaining after GBM surgical resection.
32279815	6	56	theme	>56 days	897:904	arg1	half-life					884:892	a half-life	882:892	a half-life of >56 days	882:904	In vitro, fast degrading scaffolds were fully degraded by one week, whereas slow degrading scaffolds had a half-life of >56 days.
32279815	14	57	theme	scaffold	1845:1852	arg1	profile					1866:1872	scaffold degradation profile	1845:1872	scaffold degradation profile	1845:1872	Interestingly, scaffold degradation profile did not significantly impact NSC persistence.
32279815	12	58	theme	clinical	1675:1682	arg1	approach					1684:1691	the current clinical approach	1663:1691	the current clinical approach to NSC therapy for GBM	1663:1714	NSC loaded scaffolds were compared to a direct injection (DI) of NSCs in suspension, which is the current clinical approach to NSC therapy for GBM.
32279815	12	58	theme	clinical	1675:1682	arg1	suspension					1642:1651	suspension	1642:1651	suspension	1642:1651	NSC loaded scaffolds were compared to a direct injection (DI) of NSCs in suspension, which is the current clinical approach to NSC therapy for GBM.
32279815	4	59	theme	brain	550:554	arg1	cavity					566:571	the brain resection cavity	546:571	the brain resection cavity	546:571	Here, we investigated the impact of scaffold degradation rate on NSC persistence in the brain resection cavity.
32279815	10	60	theme	cell	1358:1361	arg1	GBM8					1369:1372	GBM8	1369:1372	GBM8	1369:1372	Furthermore, secreted TRAIL was found to be highly potent against the human derived GBM cell line, GBM8, in vitro.
32279815	10	60	theme	cell	1358:1361	arg1	line					1363:1366	cell line	1358:1366	cell line	1358:1366	Furthermore, secreted TRAIL was found to be highly potent against the human derived GBM cell line, GBM8, in vitro.
32279815	9	61	theme	TRAIL	1181:1185	arg1	TCPS					1233:1236	tissue culture treated polystyrene (TCPS)	1197:1237	tissue culture treated polystyrene (TCPS)	1197:1237	Therapeutic NSCs secreting tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) had the same TRAIL output as tissue culture treated polystyrene (TCPS) when seeded on both scaffolds.
32279815	9	61	theme	TRAIL	1181:1185	arg1	output					1187:1192	the same TRAIL output	1172:1192	the same TRAIL output	1172:1192	Therapeutic NSCs secreting tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) had the same TRAIL output as tissue culture treated polystyrene (TCPS) when seeded on both scaffolds.
32279815	6	62	theme	degrading	858:866	arg1	scaffolds					868:876	slow degrading scaffolds	853:876	slow degrading scaffolds	853:876	In vitro, fast degrading scaffolds were fully degraded by one week, whereas slow degrading scaffolds had a half-life of >56 days.
32279815	8	63	theme	NSC	988:990	arg1	lines					992:996	Two different NSC lines	974:996	Two different NSC lines	974:996	Two different NSC lines readily adhered to and remained viable on Ace-DEX gelatin scaffolds, in vitro.
32279815	5	64	theme	gelatin	613:619	arg1	scaffolds					633:641	Composite acetalated dextran (Ace-DEX) gelatin electrospun scaffolds	574:641	Composite acetalated dextran (Ace-DEX) gelatin electrospun scaffolds	574:641	Composite acetalated dextran (Ace-DEX) gelatin electrospun scaffolds were fabricated with two distinct degradation profiles created by changing the ratio of cyclic to acyclic acetal coverage of Ace-DEX.
32279815	9	65	theme	culture	1204:1210	arg1	TCPS					1233:1236	tissue culture treated polystyrene (TCPS)	1197:1237	tissue culture treated polystyrene (TCPS)	1197:1237	Therapeutic NSCs secreting tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) had the same TRAIL output as tissue culture treated polystyrene (TCPS) when seeded on both scaffolds.
32279815	9	65	theme	culture	1204:1210	arg1	output					1187:1192	the same TRAIL output	1172:1192	the same TRAIL output	1172:1192	Therapeutic NSCs secreting tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) had the same TRAIL output as tissue culture treated polystyrene (TCPS) when seeded on both scaffolds.
32279815	7	66	theme	degradation	944:954	arg1	profiles					956:963	distinct degradation profiles	935:963	distinct degradation profiles	935:963	The scaffolds also retained distinct degradation profiles in vivo.
32279815	12	67	dep	loaded	1573:1578	arg1	NSC					1569:1571	NSC	1569:1571	NSC	1569:1571	NSC loaded scaffolds were compared to a direct injection (DI) of NSCs in suspension, which is the current clinical approach to NSC therapy for GBM.
32279815	9	68	theme	polystyrene	1220:1230	arg1	TCPS					1233:1236	tissue culture treated polystyrene (TCPS)	1197:1237	tissue culture treated polystyrene (TCPS)	1197:1237	Therapeutic NSCs secreting tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) had the same TRAIL output as tissue culture treated polystyrene (TCPS) when seeded on both scaffolds.
32279815	9	68	theme	polystyrene	1220:1230	arg1	output					1187:1192	the same TRAIL output	1172:1192	the same TRAIL output	1172:1192	Therapeutic NSCs secreting tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) had the same TRAIL output as tissue culture treated polystyrene (TCPS) when seeded on both scaffolds.
32279815	6	69	contain	had	878:880	arg1	scaffolds					868:876	slow degrading scaffolds	853:876	slow degrading scaffolds	853:876	In vitro, fast degrading scaffolds were fully degraded by one week, whereas slow degrading scaffolds had a half-life of >56 days.
32279815	6	69	contain	had	878:880	arg2	half-life					884:892	a half-life	882:892	a half-life of >56 days	882:904	In vitro, fast degrading scaffolds were fully degraded by one week, whereas slow degrading scaffolds had a half-life of >56 days.
32279815	16	70	theme	tumoricidal	2197:2207	arg1	therapy					2213:2219	tumoricidal NSC therapy	2197:2219	tumoricidal NSC therapy for GBM	2197:2227	Overall, these results highlight the benefit of utilizing a scaffold for application of tumoricidal NSC therapy for GBM.
32279815	5	71	theme	degradation	677:687	arg1	profiles					689:696	two distinct degradation profiles	664:696	two distinct degradation profiles created by changing the ratio of cyclic to acyclic acetal coverage of Ace-DEX	664:774	Composite acetalated dextran (Ace-DEX) gelatin electrospun scaffolds were fabricated with two distinct degradation profiles created by changing the ratio of cyclic to acyclic acetal coverage of Ace-DEX.
32279815	1	72	theme	Tumoricidal	125:135	arg1	cells					149:153	Tumoricidal neural stem cells	125:153	Tumoricidal neural stem cells (NSCs)	125:160	Tumoricidal neural stem cells (NSCs) are an emerging therapy to combat glioblastoma (GBM).
32279815	1	72	theme	Tumoricidal	125:135	arg1	therapy					178:184	an emerging therapy	166:184	an emerging therapy to combat glioblastoma (GBM)	166:213	Tumoricidal neural stem cells (NSCs) are an emerging therapy to combat glioblastoma (GBM).
32279815	1	72	theme	Tumoricidal	125:135	arg1	NSCs					156:159	NSCs	156:159	NSCs	156:159	Tumoricidal neural stem cells (NSCs) are an emerging therapy to combat glioblastoma (GBM).
32279815	16	73	theme	therapy	2213:2219	arg1	application					2182:2192	application	2182:2192	application of tumoricidal NSC therapy for GBM	2182:2227	Overall, these results highlight the benefit of utilizing a scaffold for application of tumoricidal NSC therapy for GBM.
32279815	5	74	theme	acyclic	741:747	arg1	coverage					756:763	cyclic to acyclic acetal coverage	731:763	cyclic to acyclic acetal coverage of Ace-DEX	731:774	Composite acetalated dextran (Ace-DEX) gelatin electrospun scaffolds were fabricated with two distinct degradation profiles created by changing the ratio of cyclic to acyclic acetal coverage of Ace-DEX.
32279815	1	75	theme	stem	144:147	arg1	cells					149:153	Tumoricidal neural stem cells	125:153	Tumoricidal neural stem cells (NSCs)	125:160	Tumoricidal neural stem cells (NSCs) are an emerging therapy to combat glioblastoma (GBM).
32279815	1	75	theme	stem	144:147	arg1	therapy					178:184	an emerging therapy	166:184	an emerging therapy to combat glioblastoma (GBM)	166:213	Tumoricidal neural stem cells (NSCs) are an emerging therapy to combat glioblastoma (GBM).
32279815	1	75	theme	stem	144:147	arg1	NSCs					156:159	NSCs	156:159	NSCs	156:159	Tumoricidal neural stem cells (NSCs) are an emerging therapy to combat glioblastoma (GBM).
32279815	12	76	from	injection	1616:1624	arg1	approach					1684:1691	the current clinical approach	1663:1691	the current clinical approach to NSC therapy for GBM	1663:1714	NSC loaded scaffolds were compared to a direct injection (DI) of NSCs in suspension, which is the current clinical approach to NSC therapy for GBM.
32279815	12	76	from	injection	1616:1624	arg1	suspension					1642:1651	suspension	1642:1651	suspension	1642:1651	NSC loaded scaffolds were compared to a direct injection (DI) of NSCs in suspension, which is the current clinical approach to NSC therapy for GBM.
32279815	11	77	theme	surgical	1461:1468	arg1	cavity					1480:1485	a surgical resection cavity	1459:1485	a surgical resection cavity	1459:1485	Firefly luciferase expressing NSCs were seeded on scaffolds, implanted in a surgical resection cavity and their persistence in the brain was monitored by bioluminescent imaging (BLI).
32279815	5	78	theme	coverage	756:763	arg1	ratio					722:726	the ratio	718:726	the ratio of cyclic to acyclic acetal coverage of Ace-DEX	718:774	Composite acetalated dextran (Ace-DEX) gelatin electrospun scaffolds were fabricated with two distinct degradation profiles created by changing the ratio of cyclic to acyclic acetal coverage of Ace-DEX.
32279815	15	79	theme	most	2098:2101	arg1	mice					2103:2106	most mice	2098:2106	most mice	2098:2106	However, persistence and long-term survival of NSCs was significantly greater for both scaffolds compared to DI, with scaffold implanted NSCs still detected by BLI at day 120 in most mice.
32279815	0	80	theme	glioblastoma	85:96	arg1	cavity					117:122	the glioblastoma surgical resection cavity	81:122	the glioblastoma surgical resection cavity	81:122	Impact of composite scaffold degradation rate on neural stem cell persistence in the glioblastoma surgical resection cavity.
32279815	15	81	theme	implanted	2047:2055	arg1	NSCs					2057:2060	scaffold implanted NSCs	2038:2060	scaffold implanted NSCs	2038:2060	However, persistence and long-term survival of NSCs was significantly greater for both scaffolds compared to DI, with scaffold implanted NSCs still detected by BLI at day 120 in most mice.
32279815	5	82	theme	acetalated	584:593	arg1	Ace-DEX					604:610	Ace-DEX	604:610	Ace-DEX	604:610	Composite acetalated dextran (Ace-DEX) gelatin electrospun scaffolds were fabricated with two distinct degradation profiles created by changing the ratio of cyclic to acyclic acetal coverage of Ace-DEX.
32279815	5	82	theme	acetalated	584:593	arg1	dextran					595:601	Composite acetalated dextran	574:601	Composite acetalated dextran (Ace-DEX) gelatin electrospun scaffolds	574:641	Composite acetalated dextran (Ace-DEX) gelatin electrospun scaffolds were fabricated with two distinct degradation profiles created by changing the ratio of cyclic to acyclic acetal coverage of Ace-DEX.
32279815	3	83	used	utilized	412:419	arg2	scaffolds					381:389	Biomaterial scaffolds	369:389	Biomaterial scaffolds	369:389	Biomaterial scaffolds have previously been utilized to deliver NSCs to the resection cavity.
32279815	11	84	theme	Firefly	1385:1391	arg1	luciferase					1393:1402	Firefly luciferase	1385:1402	Firefly luciferase expressing NSCs	1385:1418	Firefly luciferase expressing NSCs were seeded on scaffolds, implanted in a surgical resection cavity and their persistence in the brain was monitored by bioluminescent imaging (BLI).
32279815	0	85	theme	resection	107:115	arg1	cavity					117:122	the glioblastoma surgical resection cavity	81:122	the glioblastoma surgical resection cavity	81:122	Impact of composite scaffold degradation rate on neural stem cell persistence in the glioblastoma surgical resection cavity.
32279815	13	86	theme	implantation	1764:1775	arg1	efficiency					1777:1786	NSC implantation efficiency 2.87	1760:1791	NSC implantation efficiency 2.87	1760:1791	Fast and slow degrading scaffolds enhanced NSC implantation efficiency 2.87 and 3.08-fold over DI, respectively.
32279815	7	87	theme	distinct	935:942	arg1	profiles					956:963	distinct degradation profiles	935:963	distinct degradation profiles	935:963	The scaffolds also retained distinct degradation profiles in vivo.
32279815	5	88	theme	cyclic	731:736	arg1	coverage					756:763	cyclic to acyclic acetal coverage	731:763	cyclic to acyclic acetal coverage of Ace-DEX	731:774	Composite acetalated dextran (Ace-DEX) gelatin electrospun scaffolds were fabricated with two distinct degradation profiles created by changing the ratio of cyclic to acyclic acetal coverage of Ace-DEX.
32279815	8	89	theme	gelatin	1048:1054	arg1	scaffolds					1056:1064	Ace-DEX gelatin scaffolds	1040:1064	Ace-DEX gelatin scaffolds	1040:1064	Two different NSC lines readily adhered to and remained viable on Ace-DEX gelatin scaffolds, in vitro.
32279815	9	90	dep	necrosis	1110:1117	arg1	TRAIL					1161:1165	TRAIL	1161:1165	TRAIL	1161:1165	Therapeutic NSCs secreting tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) had the same TRAIL output as tissue culture treated polystyrene (TCPS) when seeded on both scaffolds.
32279815	9	90	dep	necrosis	1110:1117	arg1	ligand					1153:1158	factor-related apoptosis-inducing ligand	1119:1158	tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)	1104:1166	Therapeutic NSCs secreting tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) had the same TRAIL output as tissue culture treated polystyrene (TCPS) when seeded on both scaffolds.
32279815	3	91	theme	Biomaterial	369:379	arg1	scaffolds					381:389	Biomaterial scaffolds	369:389	Biomaterial scaffolds	369:389	Biomaterial scaffolds have previously been utilized to deliver NSCs to the resection cavity.
32279815	13	92	theme	degrading	1731:1739	arg1	scaffolds					1741:1749	Fast and slow degrading scaffolds	1717:1749	Fast and slow degrading scaffolds	1717:1749	Fast and slow degrading scaffolds enhanced NSC implantation efficiency 2.87 and 3.08-fold over DI, respectively.
32279815	2	93	theme	surgical	349:356	arg1	resection					358:366	GBM surgical resection	345:366	GBM surgical resection	345:366	This therapy employs genetically engineered NSCs that secrete tumoricidal agents to seek out and kill tumor foci remaining after GBM surgical resection.
32279815	0	94	theme	scaffold	20:27	arg1	rate					41:44	composite scaffold degradation rate	10:44	composite scaffold degradation rate	10:44	Impact of composite scaffold degradation rate on neural stem cell persistence in the glioblastoma surgical resection cavity.
32279815	11	95	theme	expressing	1404:1413	arg1	NSCs					1415:1418	Firefly luciferase expressing NSCs	1385:1418	Firefly luciferase expressing NSCs	1385:1418	Firefly luciferase expressing NSCs were seeded on scaffolds, implanted in a surgical resection cavity and their persistence in the brain was monitored by bioluminescent imaging (BLI).
32279815	5	96	theme	Ace-DEX	768:774	arg1	coverage					756:763	cyclic to acyclic acetal coverage	731:763	cyclic to acyclic acetal coverage of Ace-DEX	731:774	Composite acetalated dextran (Ace-DEX) gelatin electrospun scaffolds were fabricated with two distinct degradation profiles created by changing the ratio of cyclic to acyclic acetal coverage of Ace-DEX.
32279815	14	97	theme	NSC	1903:1905	arg1	persistence					1907:1917	impact NSC persistence	1896:1917	impact NSC persistence	1896:1917	Interestingly, scaffold degradation profile did not significantly impact NSC persistence.
32279815	4	98	theme	degradation	507:517	arg1	rate					519:522	scaffold degradation rate	498:522	scaffold degradation rate	498:522	Here, we investigated the impact of scaffold degradation rate on NSC persistence in the brain resection cavity.
32279815	9	99	theme	tumor	1104:1108	arg1	necrosis					1110:1117	tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)	1104:1166	tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)	1104:1166	Therapeutic NSCs secreting tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) had the same TRAIL output as tissue culture treated polystyrene (TCPS) when seeded on both scaffolds.
32279815	0	100	theme	neural	49:54	arg1	persistence					66:76	neural stem cell persistence	49:76	neural stem cell persistence in the glioblastoma surgical resection cavity	49:122	Impact of composite scaffold degradation rate on neural stem cell persistence in the glioblastoma surgical resection cavity.
32279815	9	101	theme	factor-related	1119:1132	arg1	TRAIL					1161:1165	TRAIL	1161:1165	TRAIL	1161:1165	Therapeutic NSCs secreting tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) had the same TRAIL output as tissue culture treated polystyrene (TCPS) when seeded on both scaffolds.
32279815	9	101	theme	factor-related	1119:1132	arg1	ligand					1153:1158	factor-related apoptosis-inducing ligand	1119:1158	tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)	1104:1166	Therapeutic NSCs secreting tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) had the same TRAIL output as tissue culture treated polystyrene (TCPS) when seeded on both scaffolds.
32279815	14	102	theme	degradation	1854:1864	arg1	profile					1866:1872	scaffold degradation profile	1845:1872	scaffold degradation profile	1845:1872	Interestingly, scaffold degradation profile did not significantly impact NSC persistence.
32279815	12	103	theme	NSC	1696:1698	arg1	therapy					1700:1706	NSC therapy	1696:1706	NSC therapy for GBM	1696:1714	NSC loaded scaffolds were compared to a direct injection (DI) of NSCs in suspension, which is the current clinical approach to NSC therapy for GBM.
32279815	13	104	theme	Fast	1717:1720	arg1	scaffolds					1741:1749	Fast and slow degrading scaffolds	1717:1749	Fast and slow degrading scaffolds	1717:1749	Fast and slow degrading scaffolds enhanced NSC implantation efficiency 2.87 and 3.08-fold over DI, respectively.
32279815	9	105	contain	had	1168:1170	arg2	TCPS					1233:1236	tissue culture treated polystyrene (TCPS)	1197:1237	tissue culture treated polystyrene (TCPS)	1197:1237	Therapeutic NSCs secreting tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) had the same TRAIL output as tissue culture treated polystyrene (TCPS) when seeded on both scaffolds.
32279815	9	105	contain	had	1168:1170	arg1	NSCs					1089:1092	Therapeutic NSCs	1077:1092	Therapeutic NSCs secreting tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)	1077:1166	Therapeutic NSCs secreting tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) had the same TRAIL output as tissue culture treated polystyrene (TCPS) when seeded on both scaffolds.
32279815	9	105	contain	had	1168:1170	arg2	output					1187:1192	the same TRAIL output	1172:1192	the same TRAIL output	1172:1192	Therapeutic NSCs secreting tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) had the same TRAIL output as tissue culture treated polystyrene (TCPS) when seeded on both scaffolds.
34725832	5	0	from	24-h	961:964	arg1	anti-Xa					950:956	the target anti-Xa	939:956	the target anti-Xa	939:956	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	3	1	theme	before-after	586:597	arg1	study					606:610	This retrospective before-after cohort study	567:610	This retrospective before-after cohort study	567:610	METHODS This retrospective before-after cohort study compared two groups of ECMO patients who received UFH for at least 24-h from March 2016 to May 2019.
34725832	1	2	theme	optimal	143:149	arg1	intensity					151:159	The optimal intensity	139:159	The optimal intensity of anticoagulation for adult patients supported with extracorporeal membrane oxygenation (ECMO)	139:255	OBJECTIVE The optimal intensity of anticoagulation for adult patients supported with extracorporeal membrane oxygenation (ECMO) remains uncertain.
34725832	1	3	theme	extracorporeal	214:227	arg1	ECMO					251:254	ECMO	251:254	ECMO	251:254	OBJECTIVE The optimal intensity of anticoagulation for adult patients supported with extracorporeal membrane oxygenation (ECMO) remains uncertain.
34725832	1	3	theme	extracorporeal	214:227	arg1	oxygenation					238:248	extracorporeal membrane oxygenation	214:248	extracorporeal membrane oxygenation (ECMO)	214:255	OBJECTIVE The optimal intensity of anticoagulation for adult patients supported with extracorporeal membrane oxygenation (ECMO) remains uncertain.
34725832	10	4	theme	events	1789:1794	arg1	excess					1768:1773	an excess	1765:1773	an excess of thrombotic events	1765:1794	CONCLUSION In adult ECMO patients, anticoagulation with a restricted anti-Xa target was associated with lower pRBC transfusions and did not provoke an excess of thrombotic events.
34725832	9	5	theme	patients	1560:1567	arg1	number					1550:1555	the number	1546:1555	the number of patients who achieved target anti-Xa at 24-h (p = .940)	1546:1614	There was no difference between the groups in the number of patients who achieved target anti-Xa at 24-h (p = .940).
34725832	8	6	theme	transfusion	1357:1367	arg1	rates					1318:1322	lower rates	1312:1322	lower rates of packed red blood cells (pRBC) transfusion	1312:1367	The restricted group had lower rates of packed red blood cells (pRBC) transfusion per ECMO day (mean 1 ± 1 vs 3 ± 2 units, p = .003) and required fewer heparin infusion adjustments to reach the target (p = .007).
34725832	5	7	theme	target	1059:1064	arg1	anti-Xa					1066:1072	target anti-Xa	1059:1072	target anti-Xa	1059:1072	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	3	8	theme	patients	640:647	arg1	groups					625:630	two groups	621:630	two groups of ECMO patients who received UFH for at least 24-h from March 2016 to May 2019	621:710	METHODS This retrospective before-after cohort study compared two groups of ECMO patients who received UFH for at least 24-h from March 2016 to May 2019.
34725832	3	8	theme	patients	640:647	arg1	patients					640:647	ECMO patients	635:647	ECMO patients who received UFH for at least 24-h from March 2016 to May 2019	635:710	METHODS This retrospective before-after cohort study compared two groups of ECMO patients who received UFH for at least 24-h from March 2016 to May 2019.
34725832	5	9	theme	products	862:869	arg1	amount					846:851	the mean amount	837:851	the mean amount of blood products transfused per ECMO day	837:893	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	5	9	theme	products	862:869	arg1	infusion					1029:1036	heparin infusion	1021:1036	heparin infusion	1021:1036	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	5	9	theme	products	862:869	arg1	products					862:869	blood products	856:869	blood products transfused per ECMO day	856:893	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	5	9	theme	products	862:869	arg1	time					971:974	the time	967:974	the time to achieve target anti-Xa	967:1000	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	5	9	theme	products	862:869	arg1	proportion					900:909	the proportion	896:909	the proportion of patients who were within the target anti-Xa at 24-h	896:964	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	5	9	theme	products	862:869	arg1	number					1011:1016	the number	1007:1016	the number of heparin infusion	1007:1036	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	5	9	theme	products	862:869	arg1	patients					914:921	patients	914:921	patients who were within the target anti-Xa at 24-h	914:964	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	8	10	theme	ECMO	1373:1376	arg1	day					1378:1380	ECMO day	1373:1380	ECMO day	1373:1380	The restricted group had lower rates of packed red blood cells (pRBC) transfusion per ECMO day (mean 1 ± 1 vs 3 ± 2 units, p = .003) and required fewer heparin infusion adjustments to reach the target (p = .007).
34725832	4	11	theme	composite	741:749	arg1	rate					751:754	the composite rate	737:754	the composite rate of major bleeding or thrombotic events per ECMO day	737:806	The primary outcome was the composite rate of major bleeding or thrombotic events per ECMO day.
34725832	4	11	theme	composite	741:749	arg1	outcome					725:731	The primary outcome	713:731	The primary outcome	713:731	The primary outcome was the composite rate of major bleeding or thrombotic events per ECMO day.
34725832	5	12	theme	infusion	1029:1036	arg1	amount					846:851	the mean amount	837:851	the mean amount of blood products transfused per ECMO day	837:893	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	5	12	theme	infusion	1029:1036	arg1	infusion					1029:1036	heparin infusion	1021:1036	heparin infusion	1021:1036	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	5	12	theme	infusion	1029:1036	arg1	products					862:869	blood products	856:869	blood products transfused per ECMO day	856:893	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	5	12	theme	infusion	1029:1036	arg1	time					971:974	the time	967:974	the time to achieve target anti-Xa	967:1000	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	5	12	theme	infusion	1029:1036	arg1	proportion					900:909	the proportion	896:909	the proportion of patients who were within the target anti-Xa at 24-h	896:964	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	5	12	theme	infusion	1029:1036	arg1	number					1011:1016	the number	1007:1016	the number of heparin infusion	1007:1036	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	5	12	theme	infusion	1029:1036	arg1	patients					914:921	patients	914:921	patients who were within the target anti-Xa at 24-h	914:964	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	2	13	theme	study	298:302	arg1	objective					280:288	The objective	276:288	The objective of this study	276:302	The objective of this study was to evaluate the effectiveness and safety of two anticoagulation protocols using conventional (0.3-0.7 IU/ml) versus restricted (0.2-0.5 IU/ml) anti-factor Xa (anti-Xa) targets for the management of unfractionated heparin (UFH) in adult ECMO patients.
34725832	1	14	theme	anticoagulation	164:178	arg1	intensity					151:159	The optimal intensity	139:159	The optimal intensity of anticoagulation for adult patients supported with extracorporeal membrane oxygenation (ECMO)	139:255	OBJECTIVE The optimal intensity of anticoagulation for adult patients supported with extracorporeal membrane oxygenation (ECMO) remains uncertain.
34725832	10	15	dep	CONCLUSION	1617:1626	arg1	patients					1642:1649	adult ECMO patients	1631:1649	adult ECMO patients	1631:1649	CONCLUSION In adult ECMO patients, anticoagulation with a restricted anti-Xa target was associated with lower pRBC transfusions and did not provoke an excess of thrombotic events.
34725832	2	16	from	patients	549:556	arg1	management					492:501	the management	488:501	the management of unfractionated heparin (UFH) in adult ECMO patients	488:556	The objective of this study was to evaluate the effectiveness and safety of two anticoagulation protocols using conventional (0.3-0.7 IU/ml) versus restricted (0.2-0.5 IU/ml) anti-factor Xa (anti-Xa) targets for the management of unfractionated heparin (UFH) in adult ECMO patients.
34725832	1	17	dep	OBJECTIVE	129:137	arg1	remains					257:263	remains	257:263	remains uncertain	257:273	OBJECTIVE The optimal intensity of anticoagulation for adult patients supported with extracorporeal membrane oxygenation (ECMO) remains uncertain.
34725832	5	18	theme	mean	841:844	arg1	infusion					1029:1036	heparin infusion	1021:1036	heparin infusion	1021:1036	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	5	18	theme	mean	841:844	arg1	amount					846:851	the mean amount	837:851	the mean amount of blood products transfused per ECMO day	837:893	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	5	18	theme	mean	841:844	arg1	products					862:869	blood products	856:869	blood products transfused per ECMO day	856:893	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	5	18	theme	mean	841:844	arg1	patients					914:921	patients	914:921	patients who were within the target anti-Xa at 24-h	914:964	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	1	19	theme	adult	184:188	arg1	patients					190:197	adult patients	184:197	adult patients supported with extracorporeal membrane oxygenation (ECMO)	184:255	OBJECTIVE The optimal intensity of anticoagulation for adult patients supported with extracorporeal membrane oxygenation (ECMO) remains uncertain.
34725832	8	20	theme	3 ± 2	1397:1401	arg1	units					1403:1407	3 ± 2 units	1397:1407	3 ± 2 units	1397:1407	The restricted group had lower rates of packed red blood cells (pRBC) transfusion per ECMO day (mean 1 ± 1 vs 3 ± 2 units, p = .003) and required fewer heparin infusion adjustments to reach the target (p = .007).
34725832	4	21	theme	primary	717:723	arg1	rate					751:754	the composite rate	737:754	the composite rate of major bleeding or thrombotic events per ECMO day	737:806	The primary outcome was the composite rate of major bleeding or thrombotic events per ECMO day.
34725832	4	21	theme	primary	717:723	arg1	outcome					725:731	The primary outcome	713:731	The primary outcome	713:731	The primary outcome was the composite rate of major bleeding or thrombotic events per ECMO day.
34725832	0	22	theme	extracorporeal	92:105	arg1	oxygenation					116:126	extracorporeal membrane oxygenation	92:126	extracorporeal membrane oxygenation	92:126	Conventional versus restricted anti-Xa-guided heparin protocol in adult patients undergoing extracorporeal membrane oxygenation.
34725832	4	23	theme	thrombotic	777:786	arg1	events					788:793	thrombotic events	777:793	thrombotic events	777:793	The primary outcome was the composite rate of major bleeding or thrombotic events per ECMO day.
34725832	8	24	dep	=	1491:1491	arg1	.007					1493:1496	.007	1493:1496	.007	1493:1496	The restricted group had lower rates of packed red blood cells (pRBC) transfusion per ECMO day (mean 1 ± 1 vs 3 ± 2 units, p = .003) and required fewer heparin infusion adjustments to reach the target (p = .007).
34725832	2	25	theme	ECMO	544:547	arg1	patients					549:556	adult ECMO patients	538:556	adult ECMO patients	538:556	The objective of this study was to evaluate the effectiveness and safety of two anticoagulation protocols using conventional (0.3-0.7 IU/ml) versus restricted (0.2-0.5 IU/ml) anti-factor Xa (anti-Xa) targets for the management of unfractionated heparin (UFH) in adult ECMO patients.
34725832	2	26	from	management	492:501	arg1	patients					549:556	adult ECMO patients	538:556	adult ECMO patients	538:556	The objective of this study was to evaluate the effectiveness and safety of two anticoagulation protocols using conventional (0.3-0.7 IU/ml) versus restricted (0.2-0.5 IU/ml) anti-factor Xa (anti-Xa) targets for the management of unfractionated heparin (UFH) in adult ECMO patients.
34725832	8	27	theme	restricted	1291:1300	arg1	group					1302:1306	The restricted group	1287:1306	The restricted group	1287:1306	The restricted group had lower rates of packed red blood cells (pRBC) transfusion per ECMO day (mean 1 ± 1 vs 3 ± 2 units, p = .003) and required fewer heparin infusion adjustments to reach the target (p = .007).
34725832	0	28	theme	Conventional	0:11	arg1	protocol					54:61	Conventional versus restricted anti-Xa-guided heparin protocol	0:61	protocol	54:61	Conventional versus restricted anti-Xa-guided heparin protocol in adult patients undergoing extracorporeal membrane oxygenation.
34725832	5	29	theme	target	943:948	arg1	anti-Xa					950:956	the target anti-Xa	939:956	the target anti-Xa	939:956	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	4	30	theme	bleeding	765:772	arg1	rate					751:754	the composite rate	737:754	the composite rate of major bleeding or thrombotic events per ECMO day	737:806	The primary outcome was the composite rate of major bleeding or thrombotic events per ECMO day.
34725832	4	30	theme	bleeding	765:772	arg1	outcome					725:731	The primary outcome	713:731	The primary outcome	713:731	The primary outcome was the composite rate of major bleeding or thrombotic events per ECMO day.
34725832	6	31	dep	RESULTS	1075:1081	arg1	patients					1093:1100	Forty-one patients	1083:1100	RESULTS Forty-one patients	1075:1100	RESULTS Forty-one patients were included in this analysis (conventional, n = 25; restricted, n = 16).
34725832	0	32	theme	restricted	20:29	arg1	protocol					54:61	Conventional versus restricted anti-Xa-guided heparin protocol	0:61	protocol	54:61	Conventional versus restricted anti-Xa-guided heparin protocol in adult patients undergoing extracorporeal membrane oxygenation.
34725832	2	33	theme	heparin	521:527	arg1	management					492:501	the management	488:501	the management of unfractionated heparin (UFH) in adult ECMO patients	488:556	The objective of this study was to evaluate the effectiveness and safety of two anticoagulation protocols using conventional (0.3-0.7 IU/ml) versus restricted (0.2-0.5 IU/ml) anti-factor Xa (anti-Xa) targets for the management of unfractionated heparin (UFH) in adult ECMO patients.
34725832	8	34	theme	heparin	1439:1445	arg1	adjustments					1456:1466	fewer heparin infusion adjustments	1433:1466	fewer heparin infusion adjustments	1433:1466	The restricted group had lower rates of packed red blood cells (pRBC) transfusion per ECMO day (mean 1 ± 1 vs 3 ± 2 units, p = .003) and required fewer heparin infusion adjustments to reach the target (p = .007).
34725832	8	35	theme	packed	1327:1332	arg1	pRBC					1351:1354	pRBC	1351:1354	pRBC	1351:1354	The restricted group had lower rates of packed red blood cells (pRBC) transfusion per ECMO day (mean 1 ± 1 vs 3 ± 2 units, p = .003) and required fewer heparin infusion adjustments to reach the target (p = .007).
34725832	8	35	theme	packed	1327:1332	arg1	cells					1344:1348	packed red blood cells	1327:1348	packed red blood cells (pRBC) transfusion	1327:1367	The restricted group had lower rates of packed red blood cells (pRBC) transfusion per ECMO day (mean 1 ± 1 vs 3 ± 2 units, p = .003) and required fewer heparin infusion adjustments to reach the target (p = .007).
34725832	2	36	theme	Xa	463:464	arg1	targets					476:482	conventional (0.3-0.7 IU/ml) versus restricted (0.2-0.5 IU/ml) anti-factor Xa (anti-Xa) targets	388:482	conventional (0.3-0.7 IU/ml) versus restricted (0.2-0.5 IU/ml) anti-factor Xa (anti-Xa) targets for the management of unfractionated heparin (UFH) in adult ECMO patients	388:556	The objective of this study was to evaluate the effectiveness and safety of two anticoagulation protocols using conventional (0.3-0.7 IU/ml) versus restricted (0.2-0.5 IU/ml) anti-factor Xa (anti-Xa) targets for the management of unfractionated heparin (UFH) in adult ECMO patients.
34725832	0	37	theme	heparin	46:52	arg1	protocol					54:61	Conventional versus restricted anti-Xa-guided heparin protocol	0:61	protocol	54:61	Conventional versus restricted anti-Xa-guided heparin protocol in adult patients undergoing extracorporeal membrane oxygenation.
34725832	10	38	theme	restricted	1675:1684	arg1	target					1694:1699	a restricted anti-Xa target	1673:1699	a restricted anti-Xa target	1673:1699	CONCLUSION In adult ECMO patients, anticoagulation with a restricted anti-Xa target was associated with lower pRBC transfusions and did not provoke an excess of thrombotic events.
34725832	8	39	theme	blood	1338:1342	arg1	pRBC					1351:1354	pRBC	1351:1354	pRBC	1351:1354	The restricted group had lower rates of packed red blood cells (pRBC) transfusion per ECMO day (mean 1 ± 1 vs 3 ± 2 units, p = .003) and required fewer heparin infusion adjustments to reach the target (p = .007).
34725832	8	39	theme	blood	1338:1342	arg1	cells					1344:1348	packed red blood cells	1327:1348	packed red blood cells (pRBC) transfusion	1327:1367	The restricted group had lower rates of packed red blood cells (pRBC) transfusion per ECMO day (mean 1 ± 1 vs 3 ± 2 units, p = .003) and required fewer heparin infusion adjustments to reach the target (p = .007).
34725832	2	40	theme	conventional	388:399	arg1	0.3-0.7 IU/ml					402:414	conventional (0.3-0.7 IU/ml) versus restricted (0.2-0.5 IU/ml) anti-factor Xa (anti-Xa)	388:474	0.3-0.7 IU/ml	402:414	The objective of this study was to evaluate the effectiveness and safety of two anticoagulation protocols using conventional (0.3-0.7 IU/ml) versus restricted (0.2-0.5 IU/ml) anti-factor Xa (anti-Xa) targets for the management of unfractionated heparin (UFH) in adult ECMO patients.
34725832	5	41	theme	Secondary	809:817	arg1	outcomes					819:826	Secondary outcomes	809:826	Secondary outcomes	809:826	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	7	42	theme	thrombotic	1244:1253	arg1	events					1255:1260	thrombotic events	1244:1260	thrombotic events	1244:1260	There was no difference in the composite rate of major bleeding or thrombotic events per ECMO day (p = .090).
34725832	10	43	theme	lower	1721:1725	arg1	transfusions					1732:1743	lower pRBC transfusions	1721:1743	lower pRBC transfusions	1721:1743	CONCLUSION In adult ECMO patients, anticoagulation with a restricted anti-Xa target was associated with lower pRBC transfusions and did not provoke an excess of thrombotic events.
34725832	6	44	dep	included	1107:1114	arg1	conventional					1134:1145	conventional	1134:1145	conventional	1134:1145	RESULTS Forty-one patients were included in this analysis (conventional, n = 25; restricted, n = 16).
34725832	2	45	theme	protocols	372:380	arg1	safety					342:347	safety	342:347	safety	342:347	The objective of this study was to evaluate the effectiveness and safety of two anticoagulation protocols using conventional (0.3-0.7 IU/ml) versus restricted (0.2-0.5 IU/ml) anti-factor Xa (anti-Xa) targets for the management of unfractionated heparin (UFH) in adult ECMO patients.
34725832	2	45	theme	protocols	372:380	arg1	effectiveness					324:336	the effectiveness	320:336	the effectiveness	320:336	The objective of this study was to evaluate the effectiveness and safety of two anticoagulation protocols using conventional (0.3-0.7 IU/ml) versus restricted (0.2-0.5 IU/ml) anti-factor Xa (anti-Xa) targets for the management of unfractionated heparin (UFH) in adult ECMO patients.
34725832	7	46	theme	bleeding	1232:1239	arg1	rate					1218:1221	the composite rate	1204:1221	the composite rate of major bleeding or thrombotic events	1204:1260	There was no difference in the composite rate of major bleeding or thrombotic events per ECMO day (p = .090).
34725832	3	47	from	March	689:693	arg1	24-h					679:682	24-h	679:682	24-h	679:682	METHODS This retrospective before-after cohort study compared two groups of ECMO patients who received UFH for at least 24-h from March 2016 to May 2019.
34725832	7	48	dep	day	1271:1273	arg1	p					1276:1276	p	1276:1276	p = .090	1276:1283	There was no difference in the composite rate of major bleeding or thrombotic events per ECMO day (p = .090).
34725832	2	49	theme	0.3-0.7 IU/ml	402:414	arg1	targets					476:482	conventional (0.3-0.7 IU/ml) versus restricted (0.2-0.5 IU/ml) anti-factor Xa (anti-Xa) targets	388:482	conventional (0.3-0.7 IU/ml) versus restricted (0.2-0.5 IU/ml) anti-factor Xa (anti-Xa) targets for the management of unfractionated heparin (UFH) in adult ECMO patients	388:556	The objective of this study was to evaluate the effectiveness and safety of two anticoagulation protocols using conventional (0.3-0.7 IU/ml) versus restricted (0.2-0.5 IU/ml) anti-factor Xa (anti-Xa) targets for the management of unfractionated heparin (UFH) in adult ECMO patients.
34725832	7	50	theme	composite	1208:1216	arg1	rate					1218:1221	the composite rate	1204:1221	the composite rate of major bleeding or thrombotic events	1204:1260	There was no difference in the composite rate of major bleeding or thrombotic events per ECMO day (p = .090).
34725832	3	51	theme	retrospective	572:584	arg1	study					606:610	This retrospective before-after cohort study	567:610	This retrospective before-after cohort study	567:610	METHODS This retrospective before-after cohort study compared two groups of ECMO patients who received UFH for at least 24-h from March 2016 to May 2019.
34725832	1	52	theme	membrane	229:236	arg1	ECMO					251:254	ECMO	251:254	ECMO	251:254	OBJECTIVE The optimal intensity of anticoagulation for adult patients supported with extracorporeal membrane oxygenation (ECMO) remains uncertain.
34725832	1	52	theme	membrane	229:236	arg1	oxygenation					238:248	extracorporeal membrane oxygenation	214:248	extracorporeal membrane oxygenation (ECMO)	214:255	OBJECTIVE The optimal intensity of anticoagulation for adult patients supported with extracorporeal membrane oxygenation (ECMO) remains uncertain.
34725832	5	53	theme	ECMO	886:889	arg1	day					891:893	ECMO day	886:893	ECMO day	886:893	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	6	54	dep	conventional	1134:1145	arg1	n = 16					1168:1173	n = 16	1168:1173	n = 16	1168:1173	RESULTS Forty-one patients were included in this analysis (conventional, n = 25; restricted, n = 16).
34725832	6	54	dep	conventional	1134:1145	arg1	restricted					1156:1165	restricted	1156:1165	restricted	1156:1165	RESULTS Forty-one patients were included in this analysis (conventional, n = 25; restricted, n = 16).
34725832	6	54	dep	conventional	1134:1145	arg1	n = 25					1148:1153	n = 25	1148:1153	n = 25	1148:1153	RESULTS Forty-one patients were included in this analysis (conventional, n = 25; restricted, n = 16).
34725832	3	55	theme	cohort	599:604	arg1	study					606:610	This retrospective before-after cohort study	567:610	This retrospective before-after cohort study	567:610	METHODS This retrospective before-after cohort study compared two groups of ECMO patients who received UFH for at least 24-h from March 2016 to May 2019.
34725832	10	56	theme	thrombotic	1778:1787	arg1	events					1789:1794	thrombotic events	1778:1794	thrombotic events	1778:1794	CONCLUSION In adult ECMO patients, anticoagulation with a restricted anti-Xa target was associated with lower pRBC transfusions and did not provoke an excess of thrombotic events.
34725832	8	57	dep	reach	1471:1475	arg1	p					1489:1489	p	1489:1489	p = .007	1489:1496	The restricted group had lower rates of packed red blood cells (pRBC) transfusion per ECMO day (mean 1 ± 1 vs 3 ± 2 units, p = .003) and required fewer heparin infusion adjustments to reach the target (p = .007).
34725832	3	58	theme	ECMO	635:638	arg1	patients					640:647	ECMO patients	635:647	ECMO patients who received UFH for at least 24-h from March 2016 to May 2019	635:710	METHODS This retrospective before-after cohort study compared two groups of ECMO patients who received UFH for at least 24-h from March 2016 to May 2019.
34725832	5	59	theme	blood	856:860	arg1	products					862:869	blood products	856:869	blood products transfused per ECMO day	856:893	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	9	60	from	difference	1513:1522	arg1	number					1550:1555	the number	1546:1555	the number of patients who achieved target anti-Xa at 24-h (p = .940)	1546:1614	There was no difference between the groups in the number of patients who achieved target anti-Xa at 24-h (p = .940).
34725832	10	61	theme	adult	1631:1635	arg1	patients					1642:1649	adult ECMO patients	1631:1649	adult ECMO patients	1631:1649	CONCLUSION In adult ECMO patients, anticoagulation with a restricted anti-Xa target was associated with lower pRBC transfusions and did not provoke an excess of thrombotic events.
34725832	3	62	dep	METHODS	559:565	arg1	compared					612:619	compared	612:619	compared two groups of ECMO patients who received UFH for at least 24-h from March 2016 to May 2019	612:710	METHODS This retrospective before-after cohort study compared two groups of ECMO patients who received UFH for at least 24-h from March 2016 to May 2019.
34725832	9	63	theme	target	1582:1587	arg1	anti-Xa					1589:1595	target anti-Xa	1582:1595	target anti-Xa	1582:1595	There was no difference between the groups in the number of patients who achieved target anti-Xa at 24-h (p = .940).
34725832	5	64	theme	heparin	1021:1027	arg1	infusion					1029:1036	heparin infusion	1021:1036	heparin infusion	1021:1036	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	7	65	from	difference	1190:1199	arg1	rate					1218:1221	the composite rate	1204:1221	the composite rate of major bleeding or thrombotic events	1204:1260	There was no difference in the composite rate of major bleeding or thrombotic events per ECMO day (p = .090).
34725832	0	66	theme	membrane	107:114	arg1	oxygenation					116:126	extracorporeal membrane oxygenation	92:126	extracorporeal membrane oxygenation	92:126	Conventional versus restricted anti-Xa-guided heparin protocol in adult patients undergoing extracorporeal membrane oxygenation.
34725832	8	67	contain	had	1308:1310	arg2	rates					1318:1322	lower rates	1312:1322	lower rates of packed red blood cells (pRBC) transfusion	1312:1367	The restricted group had lower rates of packed red blood cells (pRBC) transfusion per ECMO day (mean 1 ± 1 vs 3 ± 2 units, p = .003) and required fewer heparin infusion adjustments to reach the target (p = .007).
34725832	8	67	contain	had	1308:1310	arg1	group					1302:1306	The restricted group	1287:1306	The restricted group	1287:1306	The restricted group had lower rates of packed red blood cells (pRBC) transfusion per ECMO day (mean 1 ± 1 vs 3 ± 2 units, p = .003) and required fewer heparin infusion adjustments to reach the target (p = .007).
34725832	4	68	theme	ECMO	799:802	arg1	day					804:806	ECMO day	799:806	ECMO day	799:806	The primary outcome was the composite rate of major bleeding or thrombotic events per ECMO day.
34725832	7	69	theme	=	1278:1278	arg1	p					1276:1276	p	1276:1276	p = .090	1276:1283	There was no difference in the composite rate of major bleeding or thrombotic events per ECMO day (p = .090).
34725832	2	70	theme	adult	538:542	arg1	patients					549:556	adult ECMO patients	538:556	adult ECMO patients	538:556	The objective of this study was to evaluate the effectiveness and safety of two anticoagulation protocols using conventional (0.3-0.7 IU/ml) versus restricted (0.2-0.5 IU/ml) anti-factor Xa (anti-Xa) targets for the management of unfractionated heparin (UFH) in adult ECMO patients.
34725832	0	71	from	protocol	54:61	arg1	patients					72:79	adult patients	66:79	adult patients	66:79	Conventional versus restricted anti-Xa-guided heparin protocol in adult patients undergoing extracorporeal membrane oxygenation.
34725832	4	72	theme	events	788:793	arg1	rate					751:754	the composite rate	737:754	the composite rate of major bleeding or thrombotic events per ECMO day	737:806	The primary outcome was the composite rate of major bleeding or thrombotic events per ECMO day.
34725832	4	72	theme	events	788:793	arg1	outcome					725:731	The primary outcome	713:731	The primary outcome	713:731	The primary outcome was the composite rate of major bleeding or thrombotic events per ECMO day.
34725832	2	73	theme	0.2-0.5 IU/ml	436:448	arg1	Xa					463:464	conventional (0.3-0.7 IU/ml) versus restricted (0.2-0.5 IU/ml) anti-factor Xa (anti-Xa)	388:474	Xa	463:464	The objective of this study was to evaluate the effectiveness and safety of two anticoagulation protocols using conventional (0.3-0.7 IU/ml) versus restricted (0.2-0.5 IU/ml) anti-factor Xa (anti-Xa) targets for the management of unfractionated heparin (UFH) in adult ECMO patients.
34725832	8	74	theme	fewer	1433:1437	arg1	adjustments					1456:1466	fewer heparin infusion adjustments	1433:1466	fewer heparin infusion adjustments	1433:1466	The restricted group had lower rates of packed red blood cells (pRBC) transfusion per ECMO day (mean 1 ± 1 vs 3 ± 2 units, p = .003) and required fewer heparin infusion adjustments to reach the target (p = .007).
34725832	2	75	theme	restricted	424:433	arg1	Xa					463:464	conventional (0.3-0.7 IU/ml) versus restricted (0.2-0.5 IU/ml) anti-factor Xa (anti-Xa)	388:474	Xa	463:464	The objective of this study was to evaluate the effectiveness and safety of two anticoagulation protocols using conventional (0.3-0.7 IU/ml) versus restricted (0.2-0.5 IU/ml) anti-factor Xa (anti-Xa) targets for the management of unfractionated heparin (UFH) in adult ECMO patients.
34725832	0	76	theme	anti-Xa-guided	31:44	arg1	protocol					54:61	Conventional versus restricted anti-Xa-guided heparin protocol	0:61	protocol	54:61	Conventional versus restricted anti-Xa-guided heparin protocol in adult patients undergoing extracorporeal membrane oxygenation.
34725832	5	77	theme	patients	914:921	arg1	amount					846:851	the mean amount	837:851	the mean amount of blood products transfused per ECMO day	837:893	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	5	77	theme	patients	914:921	arg1	infusion					1029:1036	heparin infusion	1021:1036	heparin infusion	1021:1036	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	5	77	theme	patients	914:921	arg1	products					862:869	blood products	856:869	blood products transfused per ECMO day	856:893	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	5	77	theme	patients	914:921	arg1	time					971:974	the time	967:974	the time to achieve target anti-Xa	967:1000	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	5	77	theme	patients	914:921	arg1	proportion					900:909	the proportion	896:909	the proportion of patients who were within the target anti-Xa at 24-h	896:964	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	5	77	theme	patients	914:921	arg1	number					1011:1016	the number	1007:1016	the number of heparin infusion	1007:1036	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	5	77	theme	patients	914:921	arg1	patients					914:921	patients	914:921	patients who were within the target anti-Xa at 24-h	914:964	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	7	78	theme	ECMO	1266:1269	arg1	day					1271:1273	ECMO day	1266:1273	ECMO day (p = .090)	1266:1284	There was no difference in the composite rate of major bleeding or thrombotic events per ECMO day (p = .090).
34725832	8	79	theme	infusion	1447:1454	arg1	adjustments					1456:1466	fewer heparin infusion adjustments	1433:1466	fewer heparin infusion adjustments	1433:1466	The restricted group had lower rates of packed red blood cells (pRBC) transfusion per ECMO day (mean 1 ± 1 vs 3 ± 2 units, p = .003) and required fewer heparin infusion adjustments to reach the target (p = .007).
34725832	8	80	theme	lower	1312:1316	arg1	rates					1318:1322	lower rates	1312:1322	lower rates of packed red blood cells (pRBC) transfusion	1312:1367	The restricted group had lower rates of packed red blood cells (pRBC) transfusion per ECMO day (mean 1 ± 1 vs 3 ± 2 units, p = .003) and required fewer heparin infusion adjustments to reach the target (p = .007).
34725832	7	81	theme	events	1255:1260	arg1	rate					1218:1221	the composite rate	1204:1221	the composite rate of major bleeding or thrombotic events	1204:1260	There was no difference in the composite rate of major bleeding or thrombotic events per ECMO day (p = .090).
34725832	2	82	theme	unfractionated	506:519	arg1	UFH					530:532	UFH	530:532	UFH	530:532	The objective of this study was to evaluate the effectiveness and safety of two anticoagulation protocols using conventional (0.3-0.7 IU/ml) versus restricted (0.2-0.5 IU/ml) anti-factor Xa (anti-Xa) targets for the management of unfractionated heparin (UFH) in adult ECMO patients.
34725832	2	82	theme	unfractionated	506:519	arg1	heparin					521:527	unfractionated heparin	506:527	unfractionated heparin (UFH) in adult ECMO patients	506:556	The objective of this study was to evaluate the effectiveness and safety of two anticoagulation protocols using conventional (0.3-0.7 IU/ml) versus restricted (0.2-0.5 IU/ml) anti-factor Xa (anti-Xa) targets for the management of unfractionated heparin (UFH) in adult ECMO patients.
34725832	10	83	theme	anti-Xa	1686:1692	arg1	target					1694:1699	a restricted anti-Xa target	1673:1699	a restricted anti-Xa target	1673:1699	CONCLUSION In adult ECMO patients, anticoagulation with a restricted anti-Xa target was associated with lower pRBC transfusions and did not provoke an excess of thrombotic events.
34725832	2	84	theme	anti-factor	451:461	arg1	Xa					463:464	conventional (0.3-0.7 IU/ml) versus restricted (0.2-0.5 IU/ml) anti-factor Xa (anti-Xa)	388:474	Xa	463:464	The objective of this study was to evaluate the effectiveness and safety of two anticoagulation protocols using conventional (0.3-0.7 IU/ml) versus restricted (0.2-0.5 IU/ml) anti-factor Xa (anti-Xa) targets for the management of unfractionated heparin (UFH) in adult ECMO patients.
34725832	0	85	theme	adult	66:70	arg1	patients					72:79	adult patients	66:79	adult patients	66:79	Conventional versus restricted anti-Xa-guided heparin protocol in adult patients undergoing extracorporeal membrane oxygenation.
34725832	10	86	with	anticoagulation	1652:1666	arg1	target					1694:1699	a restricted anti-Xa target	1673:1699	a restricted anti-Xa target	1673:1699	CONCLUSION In adult ECMO patients, anticoagulation with a restricted anti-Xa target was associated with lower pRBC transfusions and did not provoke an excess of thrombotic events.
34725832	7	87	dep	=	1278:1278	arg1	.090					1280:1283	.090	1280:1283	.090	1280:1283	There was no difference in the composite rate of major bleeding or thrombotic events per ECMO day (p = .090).
34725832	2	88	from	heparin	521:527	arg1	patients					549:556	adult ECMO patients	538:556	adult ECMO patients	538:556	The objective of this study was to evaluate the effectiveness and safety of two anticoagulation protocols using conventional (0.3-0.7 IU/ml) versus restricted (0.2-0.5 IU/ml) anti-factor Xa (anti-Xa) targets for the management of unfractionated heparin (UFH) in adult ECMO patients.
34725832	10	89	theme	pRBC	1727:1730	arg1	transfusions					1732:1743	lower pRBC transfusions	1721:1743	lower pRBC transfusions	1721:1743	CONCLUSION In adult ECMO patients, anticoagulation with a restricted anti-Xa target was associated with lower pRBC transfusions and did not provoke an excess of thrombotic events.
34725832	8	90	theme	red	1334:1336	arg1	pRBC					1351:1354	pRBC	1351:1354	pRBC	1351:1354	The restricted group had lower rates of packed red blood cells (pRBC) transfusion per ECMO day (mean 1 ± 1 vs 3 ± 2 units, p = .003) and required fewer heparin infusion adjustments to reach the target (p = .007).
34725832	8	90	theme	red	1334:1336	arg1	cells					1344:1348	packed red blood cells	1327:1348	packed red blood cells (pRBC) transfusion	1327:1367	The restricted group had lower rates of packed red blood cells (pRBC) transfusion per ECMO day (mean 1 ± 1 vs 3 ± 2 units, p = .003) and required fewer heparin infusion adjustments to reach the target (p = .007).
34725832	10	91	theme	ECMO	1637:1640	arg1	patients					1642:1649	adult ECMO patients	1631:1649	adult ECMO patients	1631:1649	CONCLUSION In adult ECMO patients, anticoagulation with a restricted anti-Xa target was associated with lower pRBC transfusions and did not provoke an excess of thrombotic events.
34725832	8	92	theme	cells	1344:1348	arg1	transfusion					1357:1367	packed red blood cells (pRBC) transfusion	1327:1367	packed red blood cells (pRBC) transfusion	1327:1367	The restricted group had lower rates of packed red blood cells (pRBC) transfusion per ECMO day (mean 1 ± 1 vs 3 ± 2 units, p = .003) and required fewer heparin infusion adjustments to reach the target (p = .007).
34725832	2	93	theme	anticoagulation	356:370	arg1	protocols					372:380	two anticoagulation protocols	352:380	two anticoagulation protocols using conventional (0.3-0.7 IU/ml) versus restricted (0.2-0.5 IU/ml) anti-factor Xa (anti-Xa) targets for the management of unfractionated heparin (UFH) in adult ECMO patients	352:556	The objective of this study was to evaluate the effectiveness and safety of two anticoagulation protocols using conventional (0.3-0.7 IU/ml) versus restricted (0.2-0.5 IU/ml) anti-factor Xa (anti-Xa) targets for the management of unfractionated heparin (UFH) in adult ECMO patients.
34725832	5	94	theme	target	987:992	arg1	anti-Xa					994:1000	target anti-Xa	987:1000	target anti-Xa	987:1000	Secondary outcomes included the mean amount of blood products transfused per ECMO day, the proportion of patients who were within the target anti-Xa at 24-h, the time to achieve target anti-Xa, and the number of heparin infusion adjustments to reach target anti-Xa.
34725832	8	95	theme	=	1491:1491	arg1	p					1489:1489	p	1489:1489	p = .007	1489:1496	The restricted group had lower rates of packed red blood cells (pRBC) transfusion per ECMO day (mean 1 ± 1 vs 3 ± 2 units, p = .003) and required fewer heparin infusion adjustments to reach the target (p = .007).
34725832	8	96	dep	=	1412:1412	arg1	mean					1383:1386	mean 1 ± 1	1383:1392	mean 1 ± 1	1383:1392	The restricted group had lower rates of packed red blood cells (pRBC) transfusion per ECMO day (mean 1 ± 1 vs 3 ± 2 units, p = .003) and required fewer heparin infusion adjustments to reach the target (p = .007).
34725832	8	96	dep	=	1412:1412	arg1	units					1403:1407	3 ± 2 units	1397:1407	3 ± 2 units	1397:1407	The restricted group had lower rates of packed red blood cells (pRBC) transfusion per ECMO day (mean 1 ± 1 vs 3 ± 2 units, p = .003) and required fewer heparin infusion adjustments to reach the target (p = .007).
33789528	10	0	from	mice	1770:1773	arg1	transfer					1725:1732	transfer	1725:1732	transfer of gut microbiota from CMFG-treated mice	1725:1773	Markedly, the cohousing study revealed that transfer of gut microbiota from CMFG-treated mice largely improved the DSS-induced colitis as evidenced by reduced intestinal damage and decreased macrophages infiltration in the colon.
33789528	10	1	theme	microbiota	1741:1750	arg1	transfer					1725:1732	transfer	1725:1732	transfer of gut microbiota from CMFG-treated mice	1725:1773	Markedly, the cohousing study revealed that transfer of gut microbiota from CMFG-treated mice largely improved the DSS-induced colitis as evidenced by reduced intestinal damage and decreased macrophages infiltration in the colon.
33789528	7	2	theme	intestinal	1405:1414	arg1	damage					1416:1421	intestinal damage	1405:1421	intestinal damage	1405:1421	Our results showed that CMFG pre-supplementation alleviated DSS-induced colitis as evidenced by decreased disease activity index (DAI) score, reduced body weight loss, increased colon length and mucin secretion, and ameliorated intestinal damage.
33789528	8	3	theme	MPO	1536:1538	arg1	levels					1496:1501	decreased levels	1486:1501	decreased levels of IL-1β, IL-6, IL-8, TNF-α, and MPO in the colon and circulation	1486:1567	Moreover, CMFG reduced macrophages in the colon, resulting in decreased levels of IL-1β, IL-6, IL-8, TNF-α, and MPO in the colon and circulation.
33789528	7	4	theme	increased	1345:1353	arg1	length					1361:1366	increased colon length	1345:1366	increased colon length	1345:1366	Our results showed that CMFG pre-supplementation alleviated DSS-induced colitis as evidenced by decreased disease activity index (DAI) score, reduced body weight loss, increased colon length and mucin secretion, and ameliorated intestinal damage.
33789528	10	5	theme	intestinal	1840:1849	arg1	damage					1851:1856	intestinal damage	1840:1856	intestinal damage	1840:1856	Markedly, the cohousing study revealed that transfer of gut microbiota from CMFG-treated mice largely improved the DSS-induced colitis as evidenced by reduced intestinal damage and decreased macrophages infiltration in the colon.
33789528	8	6	from	IL-1β	1506:1510	arg1	colon					1547:1551	colon	1547:1551	colon	1547:1551	Moreover, CMFG reduced macrophages in the colon, resulting in decreased levels of IL-1β, IL-6, IL-8, TNF-α, and MPO in the colon and circulation.
33789528	8	6	from	IL-1β	1506:1510	arg1	circulation					1557:1567	circulation	1557:1567	circulation	1557:1567	Moreover, CMFG reduced macrophages in the colon, resulting in decreased levels of IL-1β, IL-6, IL-8, TNF-α, and MPO in the colon and circulation.
33789528	12	7	theme	diets	2311:2315	arg1	design					2267:2272	the rational design	2254:2272	the rational design of effective prophylactic functional diets in both animals and humans	2254:2342	Collectively, these findings demonstrate the beneficial role of CMFG in the gastrointestinal diseases, and further provide evidence for the rational design of effective prophylactic functional diets in both animals and humans.
33789528	6	8	theme	transcriptome	1056:1068	arg1	sequencing					1070:1079	transcriptome sequencing	1056:1079	transcriptome sequencing	1056:1079	At the end of the study, 16S rDNA gene amplicon sequencing, short-chain fatty acids (SCFAs) profiling, transcriptome sequencing, histological analysis, immunofluorescence staining and flow cytometry analysis were conducted.
33789528	8	9	from	levels	1496:1501	arg1	colon					1547:1551	colon	1547:1551	colon	1547:1551	Moreover, CMFG reduced macrophages in the colon, resulting in decreased levels of IL-1β, IL-6, IL-8, TNF-α, and MPO in the colon and circulation.
33789528	8	9	from	levels	1496:1501	arg1	circulation					1557:1567	circulation	1557:1567	circulation	1557:1567	Moreover, CMFG reduced macrophages in the colon, resulting in decreased levels of IL-1β, IL-6, IL-8, TNF-α, and MPO in the colon and circulation.
33789528	3	10	theme	HMOs	569:572	arg1	functions					556:564	functions	556:564	functions	556:564	Since the mixtures of fructo-oligosaccharides (FOS) and galacto-oligosaccharides (GOS) perfectly mimic the properties and functions of HMOs, the combination of MFGM, FOS, and GOS (CMFG) has therefore been developed and used in this study.
33789528	3	10	theme	HMOs	569:572	arg1	properties					541:550	the properties	537:550	the properties	537:550	Since the mixtures of fructo-oligosaccharides (FOS) and galacto-oligosaccharides (GOS) perfectly mimic the properties and functions of HMOs, the combination of MFGM, FOS, and GOS (CMFG) has therefore been developed and used in this study.
33789528	1	11	theme	milk	207:210	arg1	MFGM					234:237	MFGM	234:237	MFGM	234:237	Human milk oligosaccharides (HMOs) and milk fat globule membrane (MFGM) are highly abundant in breast milk, and have been shown to exhibit potent immunomodulatory effects.
33789528	1	11	theme	milk	207:210	arg1	membrane					224:231	milk fat globule membrane	207:231	milk fat globule membrane (MFGM)	207:238	Human milk oligosaccharides (HMOs) and milk fat globule membrane (MFGM) are highly abundant in breast milk, and have been shown to exhibit potent immunomodulatory effects.
33789528	2	12	from	role	351:354	arg1	relation					392:399	relation	392:399	relation to colitis	392:410	Yet, their role in the gut microbiota modulation in relation to colitis remains understudied.
33789528	2	12	from	role	351:354	arg1	modulation					378:387	the gut microbiota modulation	359:387	the gut microbiota modulation	359:387	Yet, their role in the gut microbiota modulation in relation to colitis remains understudied.
33789528	7	13	theme	body	1327:1330	arg1	loss					1339:1342	reduced body weight loss	1319:1342	reduced body weight loss	1319:1342	Our results showed that CMFG pre-supplementation alleviated DSS-induced colitis as evidenced by decreased disease activity index (DAI) score, reduced body weight loss, increased colon length and mucin secretion, and ameliorated intestinal damage.
33789528	10	14	theme	macrophages	1872:1882	arg1	infiltration					1884:1895	reduced intestinal damage and decreased macrophages infiltration	1832:1895	reduced intestinal damage and decreased macrophages infiltration in the colon	1832:1908	Markedly, the cohousing study revealed that transfer of gut microbiota from CMFG-treated mice largely improved the DSS-induced colitis as evidenced by reduced intestinal damage and decreased macrophages infiltration in the colon.
33789528	1	15	from	milk	270:273	arg1	abundant					251:258	abundant	251:258	abundant	251:258	Human milk oligosaccharides (HMOs) and milk fat globule membrane (MFGM) are highly abundant in breast milk, and have been shown to exhibit potent immunomodulatory effects.
33789528	11	16	theme	microbiota	1941:1950	arg1	transfer					1921:1928	transfer	1921:1928	transfer of the gut microbiota from CMFG-treated mice	1921:1973	Moreover, transfer of the gut microbiota from CMFG-treated mice protected against DSS-induced gut microbiota dysbiosis and promotes SCFAs production, which showed to be associated with colitis amelioration.
33789528	1	17	theme	globule	216:222	arg1	MFGM					234:237	MFGM	234:237	MFGM	234:237	Human milk oligosaccharides (HMOs) and milk fat globule membrane (MFGM) are highly abundant in breast milk, and have been shown to exhibit potent immunomodulatory effects.
33789528	1	17	theme	globule	216:222	arg1	membrane					224:231	milk fat globule membrane	207:231	milk fat globule membrane (MFGM)	207:238	Human milk oligosaccharides (HMOs) and milk fat globule membrane (MFGM) are highly abundant in breast milk, and have been shown to exhibit potent immunomodulatory effects.
33789528	8	18	from	IL-6	1513:1516	arg1	colon					1547:1551	colon	1547:1551	colon	1547:1551	Moreover, CMFG reduced macrophages in the colon, resulting in decreased levels of IL-1β, IL-6, IL-8, TNF-α, and MPO in the colon and circulation.
33789528	8	18	from	IL-6	1513:1516	arg1	circulation					1557:1567	circulation	1557:1567	circulation	1557:1567	Moreover, CMFG reduced macrophages in the colon, resulting in decreased levels of IL-1β, IL-6, IL-8, TNF-α, and MPO in the colon and circulation.
33789528	9	19	theme	microbiota	1604:1613	arg1	composition					1615:1625	the gut microbiota composition	1596:1625	the gut microbiota composition	1596:1625	Furthermore, CMFG altered the gut microbiota composition and promoted SCFAs production in DSS-induced colitis.
33789528	12	20	theme	prophylactic	2287:2298	arg1	diets					2311:2315	effective prophylactic functional diets	2277:2315	effective prophylactic functional diets in both animals and humans	2277:2342	Collectively, these findings demonstrate the beneficial role of CMFG in the gastrointestinal diseases, and further provide evidence for the rational design of effective prophylactic functional diets in both animals and humans.
33789528	6	21	theme	cytometry	1142:1150	arg1	analysis					1152:1159	flow cytometry analysis	1137:1159	flow cytometry analysis	1137:1159	At the end of the study, 16S rDNA gene amplicon sequencing, short-chain fatty acids (SCFAs) profiling, transcriptome sequencing, histological analysis, immunofluorescence staining and flow cytometry analysis were conducted.
33789528	6	22	theme	short-chain	1013:1023	arg1	acids					1031:1035	short-chain fatty acids	1013:1035	short-chain fatty acids (SCFAs) profiling	1013:1053	At the end of the study, 16S rDNA gene amplicon sequencing, short-chain fatty acids (SCFAs) profiling, transcriptome sequencing, histological analysis, immunofluorescence staining and flow cytometry analysis were conducted.
33789528	6	22	theme	short-chain	1013:1023	arg1	SCFAs					1038:1042	SCFAs	1038:1042	SCFAs	1038:1042	At the end of the study, 16S rDNA gene amplicon sequencing, short-chain fatty acids (SCFAs) profiling, transcriptome sequencing, histological analysis, immunofluorescence staining and flow cytometry analysis were conducted.
33789528	4	23	from	effect	751:756	arg1	DSS					785:787	DSS	785:787	DSS	785:787	Here, CMFG were pre-fed to mice for three weeks to investigate its preventive effect on dextran sodium sulfate (DSS) induced colitis.
33789528	4	23	from	effect	751:756	arg1	sulfate					776:782	dextran sodium sulfate	761:782	dextran sodium sulfate (DSS) induced colitis	761:804	Here, CMFG were pre-fed to mice for three weeks to investigate its preventive effect on dextran sodium sulfate (DSS) induced colitis.
33789528	11	24	theme	gut	2005:2007	arg1	dysbiosis					2020:2028	DSS-induced gut microbiota dysbiosis	1993:2028	DSS-induced gut microbiota dysbiosis	1993:2028	Moreover, transfer of the gut microbiota from CMFG-treated mice protected against DSS-induced gut microbiota dysbiosis and promotes SCFAs production, which showed to be associated with colitis amelioration.
33789528	10	25	theme	gut	1737:1739	arg1	microbiota					1741:1750	gut microbiota	1737:1750	gut microbiota	1737:1750	Markedly, the cohousing study revealed that transfer of gut microbiota from CMFG-treated mice largely improved the DSS-induced colitis as evidenced by reduced intestinal damage and decreased macrophages infiltration in the colon.
33789528	8	26	from	TNF-α	1525:1529	arg1	colon					1547:1551	colon	1547:1551	colon	1547:1551	Moreover, CMFG reduced macrophages in the colon, resulting in decreased levels of IL-1β, IL-6, IL-8, TNF-α, and MPO in the colon and circulation.
33789528	8	26	from	TNF-α	1525:1529	arg1	circulation					1557:1567	circulation	1557:1567	circulation	1557:1567	Moreover, CMFG reduced macrophages in the colon, resulting in decreased levels of IL-1β, IL-6, IL-8, TNF-α, and MPO in the colon and circulation.
33789528	9	27	theme	SCFAs	1640:1644	arg1	production					1646:1655	SCFAs production	1640:1655	SCFAs production in DSS-induced colitis	1640:1678	Furthermore, CMFG altered the gut microbiota composition and promoted SCFAs production in DSS-induced colitis.
33789528	12	28	theme	rational	2258:2265	arg1	design					2267:2272	the rational design	2254:2272	the rational design of effective prophylactic functional diets in both animals and humans	2254:2342	Collectively, these findings demonstrate the beneficial role of CMFG in the gastrointestinal diseases, and further provide evidence for the rational design of effective prophylactic functional diets in both animals and humans.
33789528	6	29	theme	immunofluorescence	1105:1122	arg1	staining					1124:1131	immunofluorescence staining	1105:1131	immunofluorescence staining	1105:1131	At the end of the study, 16S rDNA gene amplicon sequencing, short-chain fatty acids (SCFAs) profiling, transcriptome sequencing, histological analysis, immunofluorescence staining and flow cytometry analysis were conducted.
33789528	8	30	dep	colon	1547:1551	arg1	the					1543:1545	the	1543:1545	the	1543:1545	Moreover, CMFG reduced macrophages in the colon, resulting in decreased levels of IL-1β, IL-6, IL-8, TNF-α, and MPO in the colon and circulation.
33789528	1	31	theme	immunomodulatory	314:329	arg1	effects					331:337	potent immunomodulatory effects	307:337	potent immunomodulatory effects	307:337	Human milk oligosaccharides (HMOs) and milk fat globule membrane (MFGM) are highly abundant in breast milk, and have been shown to exhibit potent immunomodulatory effects.
33789528	1	32	theme	milk	174:177	arg1	HMOs					197:200	HMOs	197:200	HMOs	197:200	Human milk oligosaccharides (HMOs) and milk fat globule membrane (MFGM) are highly abundant in breast milk, and have been shown to exhibit potent immunomodulatory effects.
33789528	1	32	theme	milk	174:177	arg1	oligosaccharides					179:194	Human milk oligosaccharides	168:194	Human milk oligosaccharides (HMOs)	168:201	Human milk oligosaccharides (HMOs) and milk fat globule membrane (MFGM) are highly abundant in breast milk, and have been shown to exhibit potent immunomodulatory effects.
33789528	11	33	from	mice	1970:1973	arg1	transfer					1921:1928	transfer	1921:1928	transfer of the gut microbiota from CMFG-treated mice	1921:1973	Moreover, transfer of the gut microbiota from CMFG-treated mice protected against DSS-induced gut microbiota dysbiosis and promotes SCFAs production, which showed to be associated with colitis amelioration.
33789528	0	34	theme	colonic	114:120	arg1	barrier					128:134	remodeling colonic mucus barrier	103:134	remodeling colonic mucus barrier	103:134	Cohousing-mediated microbiota transfer from milk bioactive components-dosed mice ameliorate colitis by remodeling colonic mucus barrier and lamina propria macrophages.
33789528	10	35	from	infiltration	1884:1895	arg1	colon					1904:1908	the colon	1900:1908	the colon	1900:1908	Markedly, the cohousing study revealed that transfer of gut microbiota from CMFG-treated mice largely improved the DSS-induced colitis as evidenced by reduced intestinal damage and decreased macrophages infiltration in the colon.
33789528	0	36	theme	barrier	128:134	arg1	macrophages					155:165	remodeling colonic mucus barrier and lamina propria macrophages	103:165	remodeling colonic mucus barrier and lamina propria macrophages	103:165	Cohousing-mediated microbiota transfer from milk bioactive components-dosed mice ameliorate colitis by remodeling colonic mucus barrier and lamina propria macrophages.
33789528	12	37	from	design	2267:2272	arg1	humans					2337:2342	humans	2337:2342	humans	2337:2342	Collectively, these findings demonstrate the beneficial role of CMFG in the gastrointestinal diseases, and further provide evidence for the rational design of effective prophylactic functional diets in both animals and humans.
33789528	12	37	from	design	2267:2272	arg1	animals					2325:2331	animals	2325:2331	animals	2325:2331	Collectively, these findings demonstrate the beneficial role of CMFG in the gastrointestinal diseases, and further provide evidence for the rational design of effective prophylactic functional diets in both animals and humans.
33789528	7	38	theme	disease	1283:1289	arg1	DAI					1307:1309	DAI	1307:1309	DAI	1307:1309	Our results showed that CMFG pre-supplementation alleviated DSS-induced colitis as evidenced by decreased disease activity index (DAI) score, reduced body weight loss, increased colon length and mucin secretion, and ameliorated intestinal damage.
33789528	7	38	theme	disease	1283:1289	arg1	index					1300:1304	disease activity index	1283:1304	decreased disease activity index (DAI) score	1273:1316	Our results showed that CMFG pre-supplementation alleviated DSS-induced colitis as evidenced by decreased disease activity index (DAI) score, reduced body weight loss, increased colon length and mucin secretion, and ameliorated intestinal damage.
33789528	11	39	theme	colitis	2096:2102	arg1	amelioration					2104:2115	colitis amelioration	2096:2115	colitis amelioration	2096:2115	Moreover, transfer of the gut microbiota from CMFG-treated mice protected against DSS-induced gut microbiota dysbiosis and promotes SCFAs production, which showed to be associated with colitis amelioration.
33789528	2	40	theme	gut	363:365	arg1	modulation					378:387	the gut microbiota modulation	359:387	the gut microbiota modulation	359:387	Yet, their role in the gut microbiota modulation in relation to colitis remains understudied.
33789528	0	41	theme	Cohousing-mediated	0:17	arg1	transfer					30:37	Cohousing-mediated microbiota transfer	0:37	Cohousing-mediated microbiota transfer from milk bioactive components-dosed mice	0:79	Cohousing-mediated microbiota transfer from milk bioactive components-dosed mice ameliorate colitis by remodeling colonic mucus barrier and lamina propria macrophages.
33789528	7	42	theme	activity	1291:1298	arg1	DAI					1307:1309	DAI	1307:1309	DAI	1307:1309	Our results showed that CMFG pre-supplementation alleviated DSS-induced colitis as evidenced by decreased disease activity index (DAI) score, reduced body weight loss, increased colon length and mucin secretion, and ameliorated intestinal damage.
33789528	7	42	theme	activity	1291:1298	arg1	index					1300:1304	disease activity index	1283:1304	decreased disease activity index (DAI) score	1273:1316	Our results showed that CMFG pre-supplementation alleviated DSS-induced colitis as evidenced by decreased disease activity index (DAI) score, reduced body weight loss, increased colon length and mucin secretion, and ameliorated intestinal damage.
33789528	12	43	theme	beneficial	2163:2172	arg1	role					2174:2177	the beneficial role	2159:2177	the beneficial role of CMFG in the gastrointestinal diseases	2159:2218	Collectively, these findings demonstrate the beneficial role of CMFG in the gastrointestinal diseases, and further provide evidence for the rational design of effective prophylactic functional diets in both animals and humans.
33789528	10	44	theme	CMFG-treated	1757:1768	arg1	mice					1770:1773	CMFG-treated mice	1757:1773	CMFG-treated mice	1757:1773	Markedly, the cohousing study revealed that transfer of gut microbiota from CMFG-treated mice largely improved the DSS-induced colitis as evidenced by reduced intestinal damage and decreased macrophages infiltration in the colon.
33789528	0	45	theme	milk	44:47	arg1	mice					76:79	milk bioactive components-dosed mice	44:79	milk bioactive components-dosed mice	44:79	Cohousing-mediated microbiota transfer from milk bioactive components-dosed mice ameliorate colitis by remodeling colonic mucus barrier and lamina propria macrophages.
33789528	7	46	theme	DSS-induced	1237:1247	arg1	colitis					1249:1255	DSS-induced colitis	1237:1255	DSS-induced colitis	1237:1255	Our results showed that CMFG pre-supplementation alleviated DSS-induced colitis as evidenced by decreased disease activity index (DAI) score, reduced body weight loss, increased colon length and mucin secretion, and ameliorated intestinal damage.
33789528	3	47	theme	GOS	609:611	arg1	combination					579:589	the combination	575:589	the combination of MFGM, FOS, and GOS (CMFG)	575:618	Since the mixtures of fructo-oligosaccharides (FOS) and galacto-oligosaccharides (GOS) perfectly mimic the properties and functions of HMOs, the combination of MFGM, FOS, and GOS (CMFG) has therefore been developed and used in this study.
33789528	4	48	theme	dextran	761:767	arg1	DSS					785:787	DSS	785:787	DSS	785:787	Here, CMFG were pre-fed to mice for three weeks to investigate its preventive effect on dextran sodium sulfate (DSS) induced colitis.
33789528	4	48	theme	dextran	761:767	arg1	sulfate					776:782	dextran sodium sulfate	761:782	dextran sodium sulfate (DSS) induced colitis	761:804	Here, CMFG were pre-fed to mice for three weeks to investigate its preventive effect on dextran sodium sulfate (DSS) induced colitis.
33789528	0	49	theme	components-dosed	59:74	arg1	mice					76:79	milk bioactive components-dosed mice	44:79	milk bioactive components-dosed mice	44:79	Cohousing-mediated microbiota transfer from milk bioactive components-dosed mice ameliorate colitis by remodeling colonic mucus barrier and lamina propria macrophages.
33789528	8	50	theme	IL-1β	1506:1510	arg1	levels					1496:1501	decreased levels	1486:1501	decreased levels of IL-1β, IL-6, IL-8, TNF-α, and MPO in the colon and circulation	1486:1567	Moreover, CMFG reduced macrophages in the colon, resulting in decreased levels of IL-1β, IL-6, IL-8, TNF-α, and MPO in the colon and circulation.
33789528	5	51	theme	microbiota	921:930	arg1	transfer					932:939	the gut microbiota transfer	913:939	the gut microbiota transfer	913:939	Moreover, CMFG-treated and vehicle-treated mice were cohoused to further elucidate the preventive role of the gut microbiota transfer in colitis.
33789528	6	52	theme	fatty	1025:1029	arg1	acids					1031:1035	short-chain fatty acids	1013:1035	short-chain fatty acids (SCFAs) profiling	1013:1053	At the end of the study, 16S rDNA gene amplicon sequencing, short-chain fatty acids (SCFAs) profiling, transcriptome sequencing, histological analysis, immunofluorescence staining and flow cytometry analysis were conducted.
33789528	6	52	theme	fatty	1025:1029	arg1	SCFAs					1038:1042	SCFAs	1038:1042	SCFAs	1038:1042	At the end of the study, 16S rDNA gene amplicon sequencing, short-chain fatty acids (SCFAs) profiling, transcriptome sequencing, histological analysis, immunofluorescence staining and flow cytometry analysis were conducted.
33789528	8	53	theme	IL-6	1513:1516	arg1	levels					1496:1501	decreased levels	1486:1501	decreased levels of IL-1β, IL-6, IL-8, TNF-α, and MPO in the colon and circulation	1486:1567	Moreover, CMFG reduced macrophages in the colon, resulting in decreased levels of IL-1β, IL-6, IL-8, TNF-α, and MPO in the colon and circulation.
33789528	8	54	theme	IL-8	1519:1522	arg1	levels					1496:1501	decreased levels	1486:1501	decreased levels of IL-1β, IL-6, IL-8, TNF-α, and MPO in the colon and circulation	1486:1567	Moreover, CMFG reduced macrophages in the colon, resulting in decreased levels of IL-1β, IL-6, IL-8, TNF-α, and MPO in the colon and circulation.
33789528	10	55	theme	reduced	1832:1838	arg1	infiltration					1884:1895	reduced intestinal damage and decreased macrophages infiltration	1832:1895	reduced intestinal damage and decreased macrophages infiltration in the colon	1832:1908	Markedly, the cohousing study revealed that transfer of gut microbiota from CMFG-treated mice largely improved the DSS-induced colitis as evidenced by reduced intestinal damage and decreased macrophages infiltration in the colon.
33789528	3	56	theme	galacto-oligosaccharides	490:513	arg1	mixtures					444:451	the mixtures	440:451	the mixtures of fructo-oligosaccharides (FOS) and galacto-oligosaccharides (GOS)	440:519	Since the mixtures of fructo-oligosaccharides (FOS) and galacto-oligosaccharides (GOS) perfectly mimic the properties and functions of HMOs, the combination of MFGM, FOS, and GOS (CMFG) has therefore been developed and used in this study.
33789528	6	57	theme	amplicon	992:999	arg1	sequencing					1001:1010	16S rDNA gene amplicon sequencing	978:1010	16S rDNA gene amplicon sequencing	978:1010	At the end of the study, 16S rDNA gene amplicon sequencing, short-chain fatty acids (SCFAs) profiling, transcriptome sequencing, histological analysis, immunofluorescence staining and flow cytometry analysis were conducted.
33789528	8	58	theme	TNF-α	1525:1529	arg1	levels					1496:1501	decreased levels	1486:1501	decreased levels of IL-1β, IL-6, IL-8, TNF-α, and MPO in the colon and circulation	1486:1567	Moreover, CMFG reduced macrophages in the colon, resulting in decreased levels of IL-1β, IL-6, IL-8, TNF-α, and MPO in the colon and circulation.
33789528	12	59	from	humans	2337:2342	arg1	design					2267:2272	the rational design	2254:2272	the rational design of effective prophylactic functional diets in both animals and humans	2254:2342	Collectively, these findings demonstrate the beneficial role of CMFG in the gastrointestinal diseases, and further provide evidence for the rational design of effective prophylactic functional diets in both animals and humans.
33789528	5	60	from	role	905:908	arg1	colitis					944:950	colitis	944:950	colitis	944:950	Moreover, CMFG-treated and vehicle-treated mice were cohoused to further elucidate the preventive role of the gut microbiota transfer in colitis.
33789528	1	61	theme	breast	263:268	arg1	milk					270:273	breast milk	263:273	breast milk	263:273	Human milk oligosaccharides (HMOs) and milk fat globule membrane (MFGM) are highly abundant in breast milk, and have been shown to exhibit potent immunomodulatory effects.
33789528	11	62	theme	SCFAs	2043:2047	arg1	production					2049:2058	SCFAs production	2043:2058	SCFAs production	2043:2058	Moreover, transfer of the gut microbiota from CMFG-treated mice protected against DSS-induced gut microbiota dysbiosis and promotes SCFAs production, which showed to be associated with colitis amelioration.
33789528	6	63	theme	histological	1082:1093	arg1	analysis					1095:1102	histological analysis	1082:1102	histological analysis	1082:1102	At the end of the study, 16S rDNA gene amplicon sequencing, short-chain fatty acids (SCFAs) profiling, transcriptome sequencing, histological analysis, immunofluorescence staining and flow cytometry analysis were conducted.
33789528	10	64	theme	damage	1851:1856	arg1	infiltration					1884:1895	reduced intestinal damage and decreased macrophages infiltration	1832:1895	reduced intestinal damage and decreased macrophages infiltration in the colon	1832:1908	Markedly, the cohousing study revealed that transfer of gut microbiota from CMFG-treated mice largely improved the DSS-induced colitis as evidenced by reduced intestinal damage and decreased macrophages infiltration in the colon.
33789528	12	65	from	animals	2325:2331	arg1	design					2267:2272	the rational design	2254:2272	the rational design of effective prophylactic functional diets in both animals and humans	2254:2342	Collectively, these findings demonstrate the beneficial role of CMFG in the gastrointestinal diseases, and further provide evidence for the rational design of effective prophylactic functional diets in both animals and humans.
33789528	7	66	theme	mucin	1372:1376	arg1	secretion					1378:1386	mucin secretion	1372:1386	mucin secretion	1372:1386	Our results showed that CMFG pre-supplementation alleviated DSS-induced colitis as evidenced by decreased disease activity index (DAI) score, reduced body weight loss, increased colon length and mucin secretion, and ameliorated intestinal damage.
33789528	4	67	theme	sodium	769:774	arg1	DSS					785:787	DSS	785:787	DSS	785:787	Here, CMFG were pre-fed to mice for three weeks to investigate its preventive effect on dextran sodium sulfate (DSS) induced colitis.
33789528	4	67	theme	sodium	769:774	arg1	sulfate					776:782	dextran sodium sulfate	761:782	dextran sodium sulfate (DSS) induced colitis	761:804	Here, CMFG were pre-fed to mice for three weeks to investigate its preventive effect on dextran sodium sulfate (DSS) induced colitis.
33789528	7	68	theme	colon	1355:1359	arg1	length					1361:1366	increased colon length	1345:1366	increased colon length	1345:1366	Our results showed that CMFG pre-supplementation alleviated DSS-induced colitis as evidenced by decreased disease activity index (DAI) score, reduced body weight loss, increased colon length and mucin secretion, and ameliorated intestinal damage.
33789528	9	69	theme	DSS-induced	1660:1670	arg1	colitis					1672:1678	DSS-induced colitis	1660:1678	DSS-induced colitis	1660:1678	Furthermore, CMFG altered the gut microbiota composition and promoted SCFAs production in DSS-induced colitis.
33789528	12	70	from	role	2174:2177	arg1	diseases					2211:2218	the gastrointestinal diseases	2190:2218	the gastrointestinal diseases	2190:2218	Collectively, these findings demonstrate the beneficial role of CMFG in the gastrointestinal diseases, and further provide evidence for the rational design of effective prophylactic functional diets in both animals and humans.
33789528	12	71	from	diets	2311:2315	arg1	humans					2337:2342	humans	2337:2342	humans	2337:2342	Collectively, these findings demonstrate the beneficial role of CMFG in the gastrointestinal diseases, and further provide evidence for the rational design of effective prophylactic functional diets in both animals and humans.
33789528	12	71	from	diets	2311:2315	arg1	animals					2325:2331	animals	2325:2331	animals	2325:2331	Collectively, these findings demonstrate the beneficial role of CMFG in the gastrointestinal diseases, and further provide evidence for the rational design of effective prophylactic functional diets in both animals and humans.
33789528	0	72	theme	propria	147:153	arg1	macrophages					155:165	remodeling colonic mucus barrier and lamina propria macrophages	103:165	remodeling colonic mucus barrier and lamina propria macrophages	103:165	Cohousing-mediated microbiota transfer from milk bioactive components-dosed mice ameliorate colitis by remodeling colonic mucus barrier and lamina propria macrophages.
33789528	11	73	theme	gut	1937:1939	arg1	microbiota					1941:1950	the gut microbiota	1933:1950	the gut microbiota	1933:1950	Moreover, transfer of the gut microbiota from CMFG-treated mice protected against DSS-induced gut microbiota dysbiosis and promotes SCFAs production, which showed to be associated with colitis amelioration.
33789528	1	74	theme	fat	212:214	arg1	MFGM					234:237	MFGM	234:237	MFGM	234:237	Human milk oligosaccharides (HMOs) and milk fat globule membrane (MFGM) are highly abundant in breast milk, and have been shown to exhibit potent immunomodulatory effects.
33789528	1	74	theme	fat	212:214	arg1	membrane					224:231	milk fat globule membrane	207:231	milk fat globule membrane (MFGM)	207:238	Human milk oligosaccharides (HMOs) and milk fat globule membrane (MFGM) are highly abundant in breast milk, and have been shown to exhibit potent immunomodulatory effects.
33789528	11	75	theme	CMFG-treated	1957:1968	arg1	mice					1970:1973	CMFG-treated mice	1957:1973	CMFG-treated mice	1957:1973	Moreover, transfer of the gut microbiota from CMFG-treated mice protected against DSS-induced gut microbiota dysbiosis and promotes SCFAs production, which showed to be associated with colitis amelioration.
33789528	11	76	theme	DSS-induced	1993:2003	arg1	dysbiosis					2020:2028	DSS-induced gut microbiota dysbiosis	1993:2028	DSS-induced gut microbiota dysbiosis	1993:2028	Moreover, transfer of the gut microbiota from CMFG-treated mice protected against DSS-induced gut microbiota dysbiosis and promotes SCFAs production, which showed to be associated with colitis amelioration.
33789528	12	77	theme	effective	2277:2285	arg1	diets					2311:2315	effective prophylactic functional diets	2277:2315	effective prophylactic functional diets in both animals and humans	2277:2342	Collectively, these findings demonstrate the beneficial role of CMFG in the gastrointestinal diseases, and further provide evidence for the rational design of effective prophylactic functional diets in both animals and humans.
33789528	7	78	theme	weight	1332:1337	arg1	loss					1339:1342	reduced body weight loss	1319:1342	reduced body weight loss	1319:1342	Our results showed that CMFG pre-supplementation alleviated DSS-induced colitis as evidenced by decreased disease activity index (DAI) score, reduced body weight loss, increased colon length and mucin secretion, and ameliorated intestinal damage.
33789528	10	79	theme	decreased	1862:1870	arg1	macrophages					1872:1882	decreased macrophages	1862:1882	decreased macrophages	1862:1882	Markedly, the cohousing study revealed that transfer of gut microbiota from CMFG-treated mice largely improved the DSS-induced colitis as evidenced by reduced intestinal damage and decreased macrophages infiltration in the colon.
33789528	1	80	from	abundant	251:258	arg1	milk					270:273	breast milk	263:273	breast milk	263:273	Human milk oligosaccharides (HMOs) and milk fat globule membrane (MFGM) are highly abundant in breast milk, and have been shown to exhibit potent immunomodulatory effects.
33789528	9	81	theme	gut	1600:1602	arg1	composition					1615:1625	the gut microbiota composition	1596:1625	the gut microbiota composition	1596:1625	Furthermore, CMFG altered the gut microbiota composition and promoted SCFAs production in DSS-induced colitis.
33789528	12	82	theme	functional	2300:2309	arg1	diets					2311:2315	effective prophylactic functional diets	2277:2315	effective prophylactic functional diets in both animals and humans	2277:2342	Collectively, these findings demonstrate the beneficial role of CMFG in the gastrointestinal diseases, and further provide evidence for the rational design of effective prophylactic functional diets in both animals and humans.
33789528	7	83	theme	reduced	1319:1325	arg1	loss					1339:1342	reduced body weight loss	1319:1342	reduced body weight loss	1319:1342	Our results showed that CMFG pre-supplementation alleviated DSS-induced colitis as evidenced by decreased disease activity index (DAI) score, reduced body weight loss, increased colon length and mucin secretion, and ameliorated intestinal damage.
33789528	8	84	from	MPO	1536:1538	arg1	colon					1547:1551	colon	1547:1551	colon	1547:1551	Moreover, CMFG reduced macrophages in the colon, resulting in decreased levels of IL-1β, IL-6, IL-8, TNF-α, and MPO in the colon and circulation.
33789528	8	84	from	MPO	1536:1538	arg1	circulation					1557:1567	circulation	1557:1567	circulation	1557:1567	Moreover, CMFG reduced macrophages in the colon, resulting in decreased levels of IL-1β, IL-6, IL-8, TNF-α, and MPO in the colon and circulation.
33789528	10	85	theme	cohousing	1695:1703	arg1	study					1705:1709	the cohousing study	1691:1709	the cohousing study	1691:1709	Markedly, the cohousing study revealed that transfer of gut microbiota from CMFG-treated mice largely improved the DSS-induced colitis as evidenced by reduced intestinal damage and decreased macrophages infiltration in the colon.
33789528	5	86	theme	vehicle-treated	834:848	arg1	mice					850:853	CMFG-treated and vehicle-treated mice	817:853	CMFG-treated and vehicle-treated mice	817:853	Moreover, CMFG-treated and vehicle-treated mice were cohoused to further elucidate the preventive role of the gut microbiota transfer in colitis.
33789528	6	87	theme	flow	1137:1140	arg1	analysis					1152:1159	flow cytometry analysis	1137:1159	flow cytometry analysis	1137:1159	At the end of the study, 16S rDNA gene amplicon sequencing, short-chain fatty acids (SCFAs) profiling, transcriptome sequencing, histological analysis, immunofluorescence staining and flow cytometry analysis were conducted.
33789528	3	88	theme	FOS	600:602	arg1	combination					579:589	the combination	575:589	the combination of MFGM, FOS, and GOS (CMFG)	575:618	Since the mixtures of fructo-oligosaccharides (FOS) and galacto-oligosaccharides (GOS) perfectly mimic the properties and functions of HMOs, the combination of MFGM, FOS, and GOS (CMFG) has therefore been developed and used in this study.
33789528	11	89	theme	microbiota	2009:2018	arg1	dysbiosis					2020:2028	DSS-induced gut microbiota dysbiosis	1993:2028	DSS-induced gut microbiota dysbiosis	1993:2028	Moreover, transfer of the gut microbiota from CMFG-treated mice protected against DSS-induced gut microbiota dysbiosis and promotes SCFAs production, which showed to be associated with colitis amelioration.
33789528	3	90	theme	MFGM	594:597	arg1	combination					579:589	the combination	575:589	the combination of MFGM, FOS, and GOS (CMFG)	575:618	Since the mixtures of fructo-oligosaccharides (FOS) and galacto-oligosaccharides (GOS) perfectly mimic the properties and functions of HMOs, the combination of MFGM, FOS, and GOS (CMFG) has therefore been developed and used in this study.
33789528	1	91	theme	Human	168:172	arg1	HMOs					197:200	HMOs	197:200	HMOs	197:200	Human milk oligosaccharides (HMOs) and milk fat globule membrane (MFGM) are highly abundant in breast milk, and have been shown to exhibit potent immunomodulatory effects.
33789528	1	91	theme	Human	168:172	arg1	oligosaccharides					179:194	Human milk oligosaccharides	168:194	Human milk oligosaccharides (HMOs)	168:201	Human milk oligosaccharides (HMOs) and milk fat globule membrane (MFGM) are highly abundant in breast milk, and have been shown to exhibit potent immunomodulatory effects.
33789528	7	92	theme	index	1300:1304	arg1	score					1312:1316	decreased disease activity index (DAI) score	1273:1316	decreased disease activity index (DAI) score	1273:1316	Our results showed that CMFG pre-supplementation alleviated DSS-induced colitis as evidenced by decreased disease activity index (DAI) score, reduced body weight loss, increased colon length and mucin secretion, and ameliorated intestinal damage.
33789528	0	93	theme	remodeling	103:112	arg1	barrier					128:134	remodeling colonic mucus barrier	103:134	remodeling colonic mucus barrier	103:134	Cohousing-mediated microbiota transfer from milk bioactive components-dosed mice ameliorate colitis by remodeling colonic mucus barrier and lamina propria macrophages.
33789528	5	94	theme	CMFG-treated	817:828	arg1	mice					850:853	CMFG-treated and vehicle-treated mice	817:853	CMFG-treated and vehicle-treated mice	817:853	Moreover, CMFG-treated and vehicle-treated mice were cohoused to further elucidate the preventive role of the gut microbiota transfer in colitis.
33789528	12	95	theme	gastrointestinal	2194:2209	arg1	diseases					2211:2218	the gastrointestinal diseases	2190:2218	the gastrointestinal diseases	2190:2218	Collectively, these findings demonstrate the beneficial role of CMFG in the gastrointestinal diseases, and further provide evidence for the rational design of effective prophylactic functional diets in both animals and humans.
33789528	8	96	from	IL-8	1519:1522	arg1	colon					1547:1551	colon	1547:1551	colon	1547:1551	Moreover, CMFG reduced macrophages in the colon, resulting in decreased levels of IL-1β, IL-6, IL-8, TNF-α, and MPO in the colon and circulation.
33789528	8	96	from	IL-8	1519:1522	arg1	circulation					1557:1567	circulation	1557:1567	circulation	1557:1567	Moreover, CMFG reduced macrophages in the colon, resulting in decreased levels of IL-1β, IL-6, IL-8, TNF-α, and MPO in the colon and circulation.
33789528	0	97	theme	mucus	122:126	arg1	barrier					128:134	remodeling colonic mucus barrier	103:134	remodeling colonic mucus barrier	103:134	Cohousing-mediated microbiota transfer from milk bioactive components-dosed mice ameliorate colitis by remodeling colonic mucus barrier and lamina propria macrophages.
33789528	5	98	theme	preventive	894:903	arg1	role					905:908	the preventive role	890:908	the preventive role of the gut microbiota transfer in colitis	890:950	Moreover, CMFG-treated and vehicle-treated mice were cohoused to further elucidate the preventive role of the gut microbiota transfer in colitis.
33789528	0	99	from	mice	76:79	arg1	transfer					30:37	Cohousing-mediated microbiota transfer	0:37	Cohousing-mediated microbiota transfer from milk bioactive components-dosed mice	0:79	Cohousing-mediated microbiota transfer from milk bioactive components-dosed mice ameliorate colitis by remodeling colonic mucus barrier and lamina propria macrophages.
33789528	8	100	from	colon	1547:1551	arg1	levels					1496:1501	decreased levels	1486:1501	decreased levels of IL-1β, IL-6, IL-8, TNF-α, and MPO in the colon and circulation	1486:1567	Moreover, CMFG reduced macrophages in the colon, resulting in decreased levels of IL-1β, IL-6, IL-8, TNF-α, and MPO in the colon and circulation.
33789528	7	101	theme	decreased	1273:1281	arg1	score					1312:1316	decreased disease activity index (DAI) score	1273:1316	decreased disease activity index (DAI) score	1273:1316	Our results showed that CMFG pre-supplementation alleviated DSS-induced colitis as evidenced by decreased disease activity index (DAI) score, reduced body weight loss, increased colon length and mucin secretion, and ameliorated intestinal damage.
33789528	12	102	theme	CMFG	2182:2185	arg1	role					2174:2177	the beneficial role	2159:2177	the beneficial role of CMFG in the gastrointestinal diseases	2159:2218	Collectively, these findings demonstrate the beneficial role of CMFG in the gastrointestinal diseases, and further provide evidence for the rational design of effective prophylactic functional diets in both animals and humans.
33789528	2	103	theme	microbiota	367:376	arg1	modulation					378:387	the gut microbiota modulation	359:387	the gut microbiota modulation	359:387	Yet, their role in the gut microbiota modulation in relation to colitis remains understudied.
33789528	0	104	theme	microbiota	19:28	arg1	transfer					30:37	Cohousing-mediated microbiota transfer	0:37	Cohousing-mediated microbiota transfer from milk bioactive components-dosed mice	0:79	Cohousing-mediated microbiota transfer from milk bioactive components-dosed mice ameliorate colitis by remodeling colonic mucus barrier and lamina propria macrophages.
33789528	6	105	theme	16S	978:980	arg1	sequencing					1001:1010	16S rDNA gene amplicon sequencing	978:1010	16S rDNA gene amplicon sequencing	978:1010	At the end of the study, 16S rDNA gene amplicon sequencing, short-chain fatty acids (SCFAs) profiling, transcriptome sequencing, histological analysis, immunofluorescence staining and flow cytometry analysis were conducted.
33789528	6	106	theme	study	971:975	arg1	end					960:962	the end	956:962	the end of the study	956:975	At the end of the study, 16S rDNA gene amplicon sequencing, short-chain fatty acids (SCFAs) profiling, transcriptome sequencing, histological analysis, immunofluorescence staining and flow cytometry analysis were conducted.
33789528	8	107	theme	decreased	1486:1494	arg1	levels					1496:1501	decreased levels	1486:1501	decreased levels of IL-1β, IL-6, IL-8, TNF-α, and MPO in the colon and circulation	1486:1567	Moreover, CMFG reduced macrophages in the colon, resulting in decreased levels of IL-1β, IL-6, IL-8, TNF-α, and MPO in the colon and circulation.
33789528	8	108	from	circulation	1557:1567	arg1	levels					1496:1501	decreased levels	1486:1501	decreased levels of IL-1β, IL-6, IL-8, TNF-α, and MPO in the colon and circulation	1486:1567	Moreover, CMFG reduced macrophages in the colon, resulting in decreased levels of IL-1β, IL-6, IL-8, TNF-α, and MPO in the colon and circulation.
33789528	3	109	theme	fructo-oligosaccharides	456:478	arg1	mixtures					444:451	the mixtures	440:451	the mixtures of fructo-oligosaccharides (FOS) and galacto-oligosaccharides (GOS)	440:519	Since the mixtures of fructo-oligosaccharides (FOS) and galacto-oligosaccharides (GOS) perfectly mimic the properties and functions of HMOs, the combination of MFGM, FOS, and GOS (CMFG) has therefore been developed and used in this study.
33789528	0	110	theme	bioactive	49:57	arg1	mice					76:79	milk bioactive components-dosed mice	44:79	milk bioactive components-dosed mice	44:79	Cohousing-mediated microbiota transfer from milk bioactive components-dosed mice ameliorate colitis by remodeling colonic mucus barrier and lamina propria macrophages.
33789528	6	111	theme	gene	987:990	arg1	sequencing					1001:1010	16S rDNA gene amplicon sequencing	978:1010	16S rDNA gene amplicon sequencing	978:1010	At the end of the study, 16S rDNA gene amplicon sequencing, short-chain fatty acids (SCFAs) profiling, transcriptome sequencing, histological analysis, immunofluorescence staining and flow cytometry analysis were conducted.
33789528	4	112	theme	preventive	740:749	arg1	effect					751:756	its preventive effect	736:756	its preventive effect on dextran sodium sulfate (DSS) induced colitis	736:804	Here, CMFG were pre-fed to mice for three weeks to investigate its preventive effect on dextran sodium sulfate (DSS) induced colitis.
33789528	7	113	theme	CMFG	1201:1204	arg1	pre-supplementation					1206:1224	CMFG pre-supplementation	1201:1224	CMFG pre-supplementation	1201:1224	Our results showed that CMFG pre-supplementation alleviated DSS-induced colitis as evidenced by decreased disease activity index (DAI) score, reduced body weight loss, increased colon length and mucin secretion, and ameliorated intestinal damage.
33789528	10	114	theme	DSS-induced	1796:1806	arg1	colitis					1808:1814	the DSS-induced colitis	1792:1814	the DSS-induced colitis	1792:1814	Markedly, the cohousing study revealed that transfer of gut microbiota from CMFG-treated mice largely improved the DSS-induced colitis as evidenced by reduced intestinal damage and decreased macrophages infiltration in the colon.
33789528	1	115	theme	potent	307:312	arg1	effects					331:337	potent immunomodulatory effects	307:337	potent immunomodulatory effects	307:337	Human milk oligosaccharides (HMOs) and milk fat globule membrane (MFGM) are highly abundant in breast milk, and have been shown to exhibit potent immunomodulatory effects.
33789528	5	116	theme	gut	917:919	arg1	transfer					932:939	the gut microbiota transfer	913:939	the gut microbiota transfer	913:939	Moreover, CMFG-treated and vehicle-treated mice were cohoused to further elucidate the preventive role of the gut microbiota transfer in colitis.
33789528	6	117	theme	acids	1031:1035	arg1	profiling					1045:1053	short-chain fatty acids (SCFAs) profiling	1013:1053	short-chain fatty acids (SCFAs) profiling	1013:1053	At the end of the study, 16S rDNA gene amplicon sequencing, short-chain fatty acids (SCFAs) profiling, transcriptome sequencing, histological analysis, immunofluorescence staining and flow cytometry analysis were conducted.
33789528	9	118	from	production	1646:1655	arg1	colitis					1672:1678	DSS-induced colitis	1660:1678	DSS-induced colitis	1660:1678	Furthermore, CMFG altered the gut microbiota composition and promoted SCFAs production in DSS-induced colitis.
33789528	5	119	theme	transfer	932:939	arg1	role					905:908	the preventive role	890:908	the preventive role of the gut microbiota transfer in colitis	890:950	Moreover, CMFG-treated and vehicle-treated mice were cohoused to further elucidate the preventive role of the gut microbiota transfer in colitis.
33789528	0	120	theme	lamina	140:145	arg1	propria					147:153	lamina propria	140:153	lamina propria	140:153	Cohousing-mediated microbiota transfer from milk bioactive components-dosed mice ameliorate colitis by remodeling colonic mucus barrier and lamina propria macrophages.
33789528	6	121	theme	rDNA	982:985	arg1	sequencing					1001:1010	16S rDNA gene amplicon sequencing	978:1010	16S rDNA gene amplicon sequencing	978:1010	At the end of the study, 16S rDNA gene amplicon sequencing, short-chain fatty acids (SCFAs) profiling, transcriptome sequencing, histological analysis, immunofluorescence staining and flow cytometry analysis were conducted.
33789528	3	122	used	used	653:656	arg2	combination					579:589	the combination	575:589	the combination of MFGM, FOS, and GOS (CMFG)	575:618	Since the mixtures of fructo-oligosaccharides (FOS) and galacto-oligosaccharides (GOS) perfectly mimic the properties and functions of HMOs, the combination of MFGM, FOS, and GOS (CMFG) has therefore been developed and used in this study.
34163440	2	0	theme	liquid	497:502	arg1	spectroscopy					531:542	high-resolution liquid chromatography-tandem mass spectroscopy	481:542	high-resolution liquid chromatography-tandem mass spectroscopy	481:542	Here the proteome and N-glycoproteome of Chaetomium thermophilum at varying culture temperatures (30, 50, and 55°C) were studied using hydrophilic interaction liquid chromatography enrichment and high-resolution liquid chromatography-tandem mass spectroscopy analysis.
34163440	2	1	theme	culture	361:367	arg1	temperatures					369:380	varying culture temperatures	353:380	varying culture temperatures (30, 50, and 55°C)	353:399	Here the proteome and N-glycoproteome of Chaetomium thermophilum at varying culture temperatures (30, 50, and 55°C) were studied using hydrophilic interaction liquid chromatography enrichment and high-resolution liquid chromatography-tandem mass spectroscopy analysis.
34163440	1	2	theme	thermophily	243:253	arg1	basis					234:238	the underlying basis	219:238	the underlying basis of thermophily	219:253	Thermophilic fungi are eukaryotic species that grow at high temperatures, but little is known about the underlying basis of thermophily at cell and molecular levels.
34163440	2	3	theme	high-resolution	481:495	arg1	spectroscopy					531:542	high-resolution liquid chromatography-tandem mass spectroscopy	481:542	high-resolution liquid chromatography-tandem mass spectroscopy	481:542	Here the proteome and N-glycoproteome of Chaetomium thermophilum at varying culture temperatures (30, 50, and 55°C) were studied using hydrophilic interaction liquid chromatography enrichment and high-resolution liquid chromatography-tandem mass spectroscopy analysis.
34163440	0	4	from	Proteome	6:13	arg1	Response					89:96	Response	89:96	Response to High Temperature	89:116	Novel Proteome and N-Glycoproteome of the Thermophilic Fungus Chaetomium thermophilum in Response to High Temperature.
34163440	6	5	from	160	1058:1060	arg1	T55/T30					1088:1094	T55/T30	1088:1094	T55/T30	1088:1094	For the N-glycoproteome, the numbers of differentially expressed N-glycoproteins were 160, 176, and 128 in T50/T30, T55/T30, and T55/T50, respectively.
34163440	6	5	from	160	1058:1060	arg1	T55/T50					1101:1107	T55/T50	1101:1107	T55/T50	1101:1107	For the N-glycoproteome, the numbers of differentially expressed N-glycoproteins were 160, 176, and 128 in T50/T30, T55/T30, and T55/T50, respectively.
34163440	6	5	from	160	1058:1060	arg1	T50/T30					1079:1085	T50/T30	1079:1085	T50/T30	1079:1085	For the N-glycoproteome, the numbers of differentially expressed N-glycoproteins were 160, 176, and 128 in T50/T30, T55/T30, and T55/T50, respectively.
34163440	2	6	theme	varying	353:359	arg1	temperatures					369:380	varying culture temperatures	353:380	varying culture temperatures (30, 50, and 55°C)	353:399	Here the proteome and N-glycoproteome of Chaetomium thermophilum at varying culture temperatures (30, 50, and 55°C) were studied using hydrophilic interaction liquid chromatography enrichment and high-resolution liquid chromatography-tandem mass spectroscopy analysis.
34163440	6	7	theme	N-glycoproteins	1037:1051	arg1	numbers					1001:1007	the numbers	997:1007	the numbers of differentially expressed N-glycoproteins	997:1051	For the N-glycoproteome, the numbers of differentially expressed N-glycoproteins were 160, 176, and 128 in T50/T30, T55/T30, and T55/T50, respectively.
34163440	6	7	theme	N-glycoproteins	1037:1051	arg1	160					1058:1060	160	1058:1060	160	1058:1060	For the N-glycoproteome, the numbers of differentially expressed N-glycoproteins were 160, 176, and 128 in T50/T30, T55/T30, and T55/T50, respectively.
34163440	0	8	theme	High	101:104	arg1	Temperature					106:116	High Temperature	101:116	High Temperature	101:116	Novel Proteome and N-Glycoproteome of the Thermophilic Fungus Chaetomium thermophilum in Response to High Temperature.
34163440	1	9	theme	cell	258:261	arg1	levels					277:282	cell and molecular levels	258:282	levels	277:282	Thermophilic fungi are eukaryotic species that grow at high temperatures, but little is known about the underlying basis of thermophily at cell and molecular levels.
34163440	7	10	theme	mRNA	1219:1222	arg1	pathway					1237:1243	mRNA surveillance pathway	1219:1243	mRNA surveillance pathway	1219:1243	The differential glycoproteins were mainly involved in various types of N-glycan biosynthesis, mRNA surveillance pathway, and protein processing in the endoplasmic reticulum.
34163440	7	11	from	types	1187:1191	arg1	reticulum					1288:1296	the endoplasmic reticulum	1272:1296	the endoplasmic reticulum	1272:1296	The differential glycoproteins were mainly involved in various types of N-glycan biosynthesis, mRNA surveillance pathway, and protein processing in the endoplasmic reticulum.
34163440	7	12	theme	differential	1128:1139	arg1	glycoproteins					1141:1153	The differential glycoproteins	1124:1153	The differential glycoproteins	1124:1153	The differential glycoproteins were mainly involved in various types of N-glycan biosynthesis, mRNA surveillance pathway, and protein processing in the endoplasmic reticulum.
34163440	5	13	theme	complexes	961:969	arg1	constituents					914:925	structural constituents	903:925	structural constituents of the ribosome and other protein complexes	903:969	Most downregulated proteins were involved in molecular functions, including structural constituents of the ribosome and other protein complexes.
34163440	8	14	theme	essential	1378:1386	arg1	role					1388:1391	an essential role	1375:1391	an essential role	1375:1391	These results indicated that an efficient protein homeostasis pathway plays an essential role in the thermophily of C. thermophilum, and N-glycosylation is involved by affecting related proteins.
34163440	3	15	from	1,274	638:642	arg1	T55/T30					674:680	T55/T30	674:680	T55/T30	674:680	With respect to the proteome, the numbers of differentially expressed proteins were 1,274, 1,374, and 1,063 in T50/T30, T55/T30, and T55/T50, respectively.
34163440	3	15	from	1,274	638:642	arg1	T55/T50					687:693	T55/T50	687:693	T55/T50	687:693	With respect to the proteome, the numbers of differentially expressed proteins were 1,274, 1,374, and 1,063 in T50/T30, T55/T30, and T55/T50, respectively.
34163440	3	15	from	1,274	638:642	arg1	T50/T30					665:671	T50/T30	665:671	T50/T30	665:671	With respect to the proteome, the numbers of differentially expressed proteins were 1,274, 1,374, and 1,063 in T50/T30, T55/T30, and T55/T50, respectively.
34163440	3	16	dep	proteome	574:581	arg1	respect					559:565	respect	559:565	respect	559:565	With respect to the proteome, the numbers of differentially expressed proteins were 1,274, 1,374, and 1,063 in T50/T30, T55/T30, and T55/T50, respectively.
34163440	2	17	dep	proteome	294:301	arg1	the					290:292	the	290:292	the	290:292	Here the proteome and N-glycoproteome of Chaetomium thermophilum at varying culture temperatures (30, 50, and 55°C) were studied using hydrophilic interaction liquid chromatography enrichment and high-resolution liquid chromatography-tandem mass spectroscopy analysis.
34163440	9	18	theme	novel	1507:1511	arg1	This					1495:1498	This	1495:1498	This	1495:1498	This is the novel study to reveal thermophilic fungi's physiological response to high-temperature adaptation using omics analysis, facilitating the exploration of the thermophily mechanism of thermophilic fungi.
34163440	9	18	theme	novel	1507:1511	arg1	study					1513:1517	the novel study	1503:1517	the novel study to reveal thermophilic fungi's physiological response to high-temperature adaptation using omics analysis, facilitating the exploration of the thermophily mechanism of thermophilic fungi	1503:1704	This is the novel study to reveal thermophilic fungi's physiological response to high-temperature adaptation using omics analysis, facilitating the exploration of the thermophily mechanism of thermophilic fungi.
34163440	4	19	theme	protein	782:788	arg1	folding					790:796	protein folding	782:796	protein folding	782:796	The upregulated proteins were involved in biological processes, such as protein folding and carbohydrate metabolism.
34163440	1	20	theme	high	174:177	arg1	temperatures					179:190	high temperatures	174:190	high temperatures	174:190	Thermophilic fungi are eukaryotic species that grow at high temperatures, but little is known about the underlying basis of thermophily at cell and molecular levels.
34163440	2	21	theme	spectroscopy	531:542	arg1	analysis					544:551	hydrophilic interaction liquid chromatography enrichment and high-resolution liquid chromatography-tandem mass spectroscopy analysis	420:551	hydrophilic interaction liquid chromatography enrichment and high-resolution liquid chromatography-tandem mass spectroscopy analysis	420:551	Here the proteome and N-glycoproteome of Chaetomium thermophilum at varying culture temperatures (30, 50, and 55°C) were studied using hydrophilic interaction liquid chromatography enrichment and high-resolution liquid chromatography-tandem mass spectroscopy analysis.
34163440	5	22	theme	ribosome	934:941	arg1	constituents					914:925	structural constituents	903:925	structural constituents of the ribosome and other protein complexes	903:969	Most downregulated proteins were involved in molecular functions, including structural constituents of the ribosome and other protein complexes.
34163440	2	23	theme	hydrophilic	420:430	arg1	chromatography					451:464	hydrophilic interaction liquid chromatography enrichment	420:475	hydrophilic interaction liquid chromatography enrichment	420:475	Here the proteome and N-glycoproteome of Chaetomium thermophilum at varying culture temperatures (30, 50, and 55°C) were studied using hydrophilic interaction liquid chromatography enrichment and high-resolution liquid chromatography-tandem mass spectroscopy analysis.
34163440	2	24	theme	interaction	432:442	arg1	chromatography					451:464	hydrophilic interaction liquid chromatography enrichment	420:475	hydrophilic interaction liquid chromatography enrichment	420:475	Here the proteome and N-glycoproteome of Chaetomium thermophilum at varying culture temperatures (30, 50, and 55°C) were studied using hydrophilic interaction liquid chromatography enrichment and high-resolution liquid chromatography-tandem mass spectroscopy analysis.
34163440	2	25	theme	mass	526:529	arg1	spectroscopy					531:542	high-resolution liquid chromatography-tandem mass spectroscopy	481:542	high-resolution liquid chromatography-tandem mass spectroscopy	481:542	Here the proteome and N-glycoproteome of Chaetomium thermophilum at varying culture temperatures (30, 50, and 55°C) were studied using hydrophilic interaction liquid chromatography enrichment and high-resolution liquid chromatography-tandem mass spectroscopy analysis.
34163440	9	26	theme	fungi	1700:1704	arg1	mechanism					1674:1682	the thermophily mechanism	1658:1682	the thermophily mechanism of thermophilic fungi	1658:1704	This is the novel study to reveal thermophilic fungi's physiological response to high-temperature adaptation using omics analysis, facilitating the exploration of the thermophily mechanism of thermophilic fungi.
34163440	0	27	theme	Novel	0:4	arg1	Proteome					6:13	Novel Proteome	0:13	Novel Proteome	0:13	Novel Proteome and N-Glycoproteome of the Thermophilic Fungus Chaetomium thermophilum in Response to High Temperature.
34163440	1	28	theme	molecular	267:275	arg1	levels					277:282	cell and molecular levels	258:282	levels	277:282	Thermophilic fungi are eukaryotic species that grow at high temperatures, but little is known about the underlying basis of thermophily at cell and molecular levels.
34163440	5	29	dep	ribosome	934:941	arg1	the					930:932	the	930:932	the	930:932	Most downregulated proteins were involved in molecular functions, including structural constituents of the ribosome and other protein complexes.
34163440	5	30	theme	structural	903:912	arg1	constituents					914:925	structural constituents	903:925	structural constituents of the ribosome and other protein complexes	903:969	Most downregulated proteins were involved in molecular functions, including structural constituents of the ribosome and other protein complexes.
34163440	5	31	theme	downregulated	832:844	arg1	proteins					846:853	Most downregulated proteins	827:853	Most downregulated proteins	827:853	Most downregulated proteins were involved in molecular functions, including structural constituents of the ribosome and other protein complexes.
34163440	5	32	theme	Most	827:830	arg1	proteins					846:853	Most downregulated proteins	827:853	Most downregulated proteins	827:853	Most downregulated proteins were involved in molecular functions, including structural constituents of the ribosome and other protein complexes.
34163440	8	33	theme	efficient	1331:1339	arg1	pathway					1361:1367	an efficient protein homeostasis pathway	1328:1367	an efficient protein homeostasis pathway	1328:1367	These results indicated that an efficient protein homeostasis pathway plays an essential role in the thermophily of C. thermophilum, and N-glycosylation is involved by affecting related proteins.
34163440	2	34	from	temperatures	369:380	arg1	proteome					294:301	proteome	294:301	proteome	294:301	Here the proteome and N-glycoproteome of Chaetomium thermophilum at varying culture temperatures (30, 50, and 55°C) were studied using hydrophilic interaction liquid chromatography enrichment and high-resolution liquid chromatography-tandem mass spectroscopy analysis.
34163440	2	34	from	temperatures	369:380	arg1	N-glycoproteome					307:321	N-glycoproteome	307:321	N-glycoproteome	307:321	Here the proteome and N-glycoproteome of Chaetomium thermophilum at varying culture temperatures (30, 50, and 55°C) were studied using hydrophilic interaction liquid chromatography enrichment and high-resolution liquid chromatography-tandem mass spectroscopy analysis.
34163440	8	35	theme	thermophilum	1418:1429	arg1	thermophily					1400:1410	the thermophily	1396:1410	the thermophily of C. thermophilum	1396:1429	These results indicated that an efficient protein homeostasis pathway plays an essential role in the thermophily of C. thermophilum, and N-glycosylation is involved by affecting related proteins.
34163440	8	36	theme	homeostasis	1349:1359	arg1	pathway					1361:1367	an efficient protein homeostasis pathway	1328:1367	an efficient protein homeostasis pathway	1328:1367	These results indicated that an efficient protein homeostasis pathway plays an essential role in the thermophily of C. thermophilum, and N-glycosylation is involved by affecting related proteins.
34163440	9	37	theme	omics	1610:1614	arg1	analysis					1616:1623	omics analysis	1610:1623	omics analysis	1610:1623	This is the novel study to reveal thermophilic fungi's physiological response to high-temperature adaptation using omics analysis, facilitating the exploration of the thermophily mechanism of thermophilic fungi.
34163440	0	38	from	Response	89:96	arg1	N-Glycoproteome					19:33	N-Glycoproteome	19:33	N-Glycoproteome	19:33	Novel Proteome and N-Glycoproteome of the Thermophilic Fungus Chaetomium thermophilum in Response to High Temperature.
34163440	0	38	from	Response	89:96	arg1	Proteome					6:13	Novel Proteome	0:13	Novel Proteome	0:13	Novel Proteome and N-Glycoproteome of the Thermophilic Fungus Chaetomium thermophilum in Response to High Temperature.
34163440	4	39	theme	biological	752:761	arg1	folding					790:796	protein folding	782:796	protein folding	782:796	The upregulated proteins were involved in biological processes, such as protein folding and carbohydrate metabolism.
34163440	4	39	theme	biological	752:761	arg1	processes					763:771	biological processes	752:771	biological processes	752:771	The upregulated proteins were involved in biological processes, such as protein folding and carbohydrate metabolism.
34163440	4	39	theme	biological	752:761	arg1	metabolism					815:824	carbohydrate metabolism	802:824	carbohydrate metabolism	802:824	The upregulated proteins were involved in biological processes, such as protein folding and carbohydrate metabolism.
34163440	9	40	theme	high-temperature	1576:1591	arg1	adaptation					1593:1602	high-temperature adaptation	1576:1602	high-temperature adaptation	1576:1602	This is the novel study to reveal thermophilic fungi's physiological response to high-temperature adaptation using omics analysis, facilitating the exploration of the thermophily mechanism of thermophilic fungi.
34163440	7	41	gly	glycoproteins	1141:1153	arg1	glycoproteins					1141:1153	The differential glycoproteins	1124:1153	The differential glycoproteins	1124:1153	The differential glycoproteins were mainly involved in various types of N-glycan biosynthesis, mRNA surveillance pathway, and protein processing in the endoplasmic reticulum.
34163440	0	42	theme	Chaetomium	62:71	arg1	thermophilum					73:84	the Thermophilic Fungus Chaetomium thermophilum	38:84	the Thermophilic Fungus Chaetomium thermophilum in Response to High Temperature	38:116	Novel Proteome and N-Glycoproteome of the Thermophilic Fungus Chaetomium thermophilum in Response to High Temperature.
34163440	9	43	theme	thermophilic	1529:1540	arg1	response					1564:1571	thermophilic fungi's physiological response	1529:1571	thermophilic fungi's physiological response to high-temperature adaptation	1529:1602	This is the novel study to reveal thermophilic fungi's physiological response to high-temperature adaptation using omics analysis, facilitating the exploration of the thermophily mechanism of thermophilic fungi.
34163440	7	44	theme	biosynthesis	1205:1216	arg1	types					1187:1191	various types	1179:1191	various types of N-glycan biosynthesis, mRNA surveillance pathway, and protein processing in the endoplasmic reticulum	1179:1296	The differential glycoproteins were mainly involved in various types of N-glycan biosynthesis, mRNA surveillance pathway, and protein processing in the endoplasmic reticulum.
34163440	3	45	theme	proteins	624:631	arg1	numbers					588:594	the numbers	584:594	the numbers of differentially expressed proteins	584:631	With respect to the proteome, the numbers of differentially expressed proteins were 1,274, 1,374, and 1,063 in T50/T30, T55/T30, and T55/T50, respectively.
34163440	3	45	theme	proteins	624:631	arg1	1,274					638:642	1,274	638:642	1,274	638:642	With respect to the proteome, the numbers of differentially expressed proteins were 1,274, 1,374, and 1,063 in T50/T30, T55/T30, and T55/T50, respectively.
34163440	8	46	theme	protein	1341:1347	arg1	pathway					1361:1367	an efficient protein homeostasis pathway	1328:1367	an efficient protein homeostasis pathway	1328:1367	These results indicated that an efficient protein homeostasis pathway plays an essential role in the thermophily of C. thermophilum, and N-glycosylation is involved by affecting related proteins.
34163440	2	47	theme	enrichment	466:475	arg1	chromatography					451:464	hydrophilic interaction liquid chromatography enrichment	420:475	hydrophilic interaction liquid chromatography enrichment	420:475	Here the proteome and N-glycoproteome of Chaetomium thermophilum at varying culture temperatures (30, 50, and 55°C) were studied using hydrophilic interaction liquid chromatography enrichment and high-resolution liquid chromatography-tandem mass spectroscopy analysis.
34163440	7	48	theme	various	1179:1185	arg1	types					1187:1191	various types	1179:1191	various types of N-glycan biosynthesis, mRNA surveillance pathway, and protein processing in the endoplasmic reticulum	1179:1296	The differential glycoproteins were mainly involved in various types of N-glycan biosynthesis, mRNA surveillance pathway, and protein processing in the endoplasmic reticulum.
34163440	0	49	from	thermophilum	73:84	arg1	Response					89:96	Response	89:96	Response to High Temperature	89:116	Novel Proteome and N-Glycoproteome of the Thermophilic Fungus Chaetomium thermophilum in Response to High Temperature.
34163440	5	50	theme	other	947:951	arg1	complexes					961:969	other protein complexes	947:969	other protein complexes	947:969	Most downregulated proteins were involved in molecular functions, including structural constituents of the ribosome and other protein complexes.
34163440	6	51	gly	N-glycoproteins	1037:1051	arg1	N-glycoproteins					1037:1051	differentially expressed N-glycoproteins	1012:1051	differentially expressed N-glycoproteins	1012:1051	For the N-glycoproteome, the numbers of differentially expressed N-glycoproteins were 160, 176, and 128 in T50/T30, T55/T30, and T55/T50, respectively.
34163440	5	52	theme	molecular	872:880	arg1	constituents					914:925	structural constituents	903:925	structural constituents of the ribosome and other protein complexes	903:969	Most downregulated proteins were involved in molecular functions, including structural constituents of the ribosome and other protein complexes.
34163440	5	52	theme	molecular	872:880	arg1	functions					882:890	molecular functions	872:890	molecular functions	872:890	Most downregulated proteins were involved in molecular functions, including structural constituents of the ribosome and other protein complexes.
34163440	2	53	theme	chromatography	451:464	arg1	analysis					544:551	hydrophilic interaction liquid chromatography enrichment and high-resolution liquid chromatography-tandem mass spectroscopy analysis	420:551	hydrophilic interaction liquid chromatography enrichment and high-resolution liquid chromatography-tandem mass spectroscopy analysis	420:551	Here the proteome and N-glycoproteome of Chaetomium thermophilum at varying culture temperatures (30, 50, and 55°C) were studied using hydrophilic interaction liquid chromatography enrichment and high-resolution liquid chromatography-tandem mass spectroscopy analysis.
34163440	7	54	theme	surveillance	1224:1235	arg1	pathway					1237:1243	mRNA surveillance pathway	1219:1243	mRNA surveillance pathway	1219:1243	The differential glycoproteins were mainly involved in various types of N-glycan biosynthesis, mRNA surveillance pathway, and protein processing in the endoplasmic reticulum.
34163440	2	55	theme	chromatography-tandem	504:524	arg1	spectroscopy					531:542	high-resolution liquid chromatography-tandem mass spectroscopy	481:542	high-resolution liquid chromatography-tandem mass spectroscopy	481:542	Here the proteome and N-glycoproteome of Chaetomium thermophilum at varying culture temperatures (30, 50, and 55°C) were studied using hydrophilic interaction liquid chromatography enrichment and high-resolution liquid chromatography-tandem mass spectroscopy analysis.
34163440	9	56	theme	thermophily	1662:1672	arg1	mechanism					1674:1682	the thermophily mechanism	1658:1682	the thermophily mechanism of thermophilic fungi	1658:1704	This is the novel study to reveal thermophilic fungi's physiological response to high-temperature adaptation using omics analysis, facilitating the exploration of the thermophily mechanism of thermophilic fungi.
34163440	1	57	theme	Thermophilic	119:130	arg1	fungi					132:136	Thermophilic fungi	119:136	Thermophilic fungi	119:136	Thermophilic fungi are eukaryotic species that grow at high temperatures, but little is known about the underlying basis of thermophily at cell and molecular levels.
34163440	1	57	theme	Thermophilic	119:130	arg1	species					153:159	eukaryotic species	142:159	eukaryotic species that grow at high temperatures	142:190	Thermophilic fungi are eukaryotic species that grow at high temperatures, but little is known about the underlying basis of thermophily at cell and molecular levels.
34163440	0	58	theme	Thermophilic	42:53	arg1	thermophilum					73:84	the Thermophilic Fungus Chaetomium thermophilum	38:84	the Thermophilic Fungus Chaetomium thermophilum in Response to High Temperature	38:116	Novel Proteome and N-Glycoproteome of the Thermophilic Fungus Chaetomium thermophilum in Response to High Temperature.
34163440	7	59	theme	protein	1250:1256	arg1	processing					1258:1267	protein processing	1250:1267	protein processing	1250:1267	The differential glycoproteins were mainly involved in various types of N-glycan biosynthesis, mRNA surveillance pathway, and protein processing in the endoplasmic reticulum.
34163440	2	60	theme	liquid	444:449	arg1	chromatography					451:464	hydrophilic interaction liquid chromatography enrichment	420:475	hydrophilic interaction liquid chromatography enrichment	420:475	Here the proteome and N-glycoproteome of Chaetomium thermophilum at varying culture temperatures (30, 50, and 55°C) were studied using hydrophilic interaction liquid chromatography enrichment and high-resolution liquid chromatography-tandem mass spectroscopy analysis.
34163440	3	61	theme	expressed	614:622	arg1	proteins					624:631	differentially expressed proteins	599:631	differentially expressed proteins	599:631	With respect to the proteome, the numbers of differentially expressed proteins were 1,274, 1,374, and 1,063 in T50/T30, T55/T30, and T55/T50, respectively.
34163440	2	62	theme	thermophilum	337:348	arg1	proteome					294:301	proteome	294:301	proteome	294:301	Here the proteome and N-glycoproteome of Chaetomium thermophilum at varying culture temperatures (30, 50, and 55°C) were studied using hydrophilic interaction liquid chromatography enrichment and high-resolution liquid chromatography-tandem mass spectroscopy analysis.
34163440	2	62	theme	thermophilum	337:348	arg1	N-glycoproteome					307:321	N-glycoproteome	307:321	N-glycoproteome	307:321	Here the proteome and N-glycoproteome of Chaetomium thermophilum at varying culture temperatures (30, 50, and 55°C) were studied using hydrophilic interaction liquid chromatography enrichment and high-resolution liquid chromatography-tandem mass spectroscopy analysis.
34163440	4	63	theme	upregulated	714:724	arg1	proteins					726:733	The upregulated proteins	710:733	The upregulated proteins	710:733	The upregulated proteins were involved in biological processes, such as protein folding and carbohydrate metabolism.
34163440	5	64	theme	protein	953:959	arg1	complexes					961:969	other protein complexes	947:969	other protein complexes	947:969	Most downregulated proteins were involved in molecular functions, including structural constituents of the ribosome and other protein complexes.
34163440	6	65	theme	expressed	1027:1035	arg1	N-glycoproteins					1037:1051	differentially expressed N-glycoproteins	1012:1051	differentially expressed N-glycoproteins	1012:1051	For the N-glycoproteome, the numbers of differentially expressed N-glycoproteins were 160, 176, and 128 in T50/T30, T55/T30, and T55/T50, respectively.
34163440	2	66	dep	temperatures	369:380	arg1	50					387:388	50	387:388	50	387:388	Here the proteome and N-glycoproteome of Chaetomium thermophilum at varying culture temperatures (30, 50, and 55°C) were studied using hydrophilic interaction liquid chromatography enrichment and high-resolution liquid chromatography-tandem mass spectroscopy analysis.
34163440	2	66	dep	temperatures	369:380	arg1	55°C					395:398	55°C	395:398	55°C	395:398	Here the proteome and N-glycoproteome of Chaetomium thermophilum at varying culture temperatures (30, 50, and 55°C) were studied using hydrophilic interaction liquid chromatography enrichment and high-resolution liquid chromatography-tandem mass spectroscopy analysis.
34163440	2	66	dep	temperatures	369:380	arg1	30					383:384	30	383:384	30	383:384	Here the proteome and N-glycoproteome of Chaetomium thermophilum at varying culture temperatures (30, 50, and 55°C) were studied using hydrophilic interaction liquid chromatography enrichment and high-resolution liquid chromatography-tandem mass spectroscopy analysis.
34163440	9	67	theme	thermophilic	1687:1698	arg1	fungi					1700:1704	thermophilic fungi	1687:1704	thermophilic fungi	1687:1704	This is the novel study to reveal thermophilic fungi's physiological response to high-temperature adaptation using omics analysis, facilitating the exploration of the thermophily mechanism of thermophilic fungi.
34163440	1	68	theme	eukaryotic	142:151	arg1	fungi					132:136	Thermophilic fungi	119:136	Thermophilic fungi	119:136	Thermophilic fungi are eukaryotic species that grow at high temperatures, but little is known about the underlying basis of thermophily at cell and molecular levels.
34163440	1	68	theme	eukaryotic	142:151	arg1	species					153:159	eukaryotic species	142:159	eukaryotic species that grow at high temperatures	142:190	Thermophilic fungi are eukaryotic species that grow at high temperatures, but little is known about the underlying basis of thermophily at cell and molecular levels.
34163440	1	69	theme	underlying	223:232	arg1	basis					234:238	the underlying basis	219:238	the underlying basis of thermophily	219:253	Thermophilic fungi are eukaryotic species that grow at high temperatures, but little is known about the underlying basis of thermophily at cell and molecular levels.
34163440	0	70	theme	thermophilum	73:84	arg1	N-Glycoproteome					19:33	N-Glycoproteome	19:33	N-Glycoproteome	19:33	Novel Proteome and N-Glycoproteome of the Thermophilic Fungus Chaetomium thermophilum in Response to High Temperature.
34163440	0	70	theme	thermophilum	73:84	arg1	Proteome					6:13	Novel Proteome	0:13	Novel Proteome	0:13	Novel Proteome and N-Glycoproteome of the Thermophilic Fungus Chaetomium thermophilum in Response to High Temperature.
34163440	8	71	theme	related	1477:1483	arg1	proteins					1485:1492	related proteins	1477:1492	related proteins	1477:1492	These results indicated that an efficient protein homeostasis pathway plays an essential role in the thermophily of C. thermophilum, and N-glycosylation is involved by affecting related proteins.
34163440	9	72	theme	physiological	1550:1562	arg1	response					1564:1571	thermophilic fungi's physiological response	1529:1571	thermophilic fungi's physiological response to high-temperature adaptation	1529:1602	This is the novel study to reveal thermophilic fungi's physiological response to high-temperature adaptation using omics analysis, facilitating the exploration of the thermophily mechanism of thermophilic fungi.
34163440	7	73	theme	endoplasmic	1276:1286	arg1	reticulum					1288:1296	the endoplasmic reticulum	1272:1296	the endoplasmic reticulum	1272:1296	The differential glycoproteins were mainly involved in various types of N-glycan biosynthesis, mRNA surveillance pathway, and protein processing in the endoplasmic reticulum.
34163440	7	74	theme	processing	1258:1267	arg1	types					1187:1191	various types	1179:1191	various types of N-glycan biosynthesis, mRNA surveillance pathway, and protein processing in the endoplasmic reticulum	1179:1296	The differential glycoproteins were mainly involved in various types of N-glycan biosynthesis, mRNA surveillance pathway, and protein processing in the endoplasmic reticulum.
34163440	0	75	theme	Fungus	55:60	arg1	thermophilum					73:84	the Thermophilic Fungus Chaetomium thermophilum	38:84	the Thermophilic Fungus Chaetomium thermophilum in Response to High Temperature	38:116	Novel Proteome and N-Glycoproteome of the Thermophilic Fungus Chaetomium thermophilum in Response to High Temperature.
34163440	0	76	from	N-Glycoproteome	19:33	arg1	Response					89:96	Response	89:96	Response to High Temperature	89:116	Novel Proteome and N-Glycoproteome of the Thermophilic Fungus Chaetomium thermophilum in Response to High Temperature.
34163440	4	77	theme	carbohydrate	802:813	arg1	metabolism					815:824	carbohydrate metabolism	802:824	carbohydrate metabolism	802:824	The upregulated proteins were involved in biological processes, such as protein folding and carbohydrate metabolism.
34163440	7	78	theme	N-glycan	1196:1203	arg1	biosynthesis					1205:1216	N-glycan biosynthesis	1196:1216	N-glycan biosynthesis	1196:1216	The differential glycoproteins were mainly involved in various types of N-glycan biosynthesis, mRNA surveillance pathway, and protein processing in the endoplasmic reticulum.
34163440	9	79	theme	mechanism	1674:1682	arg1	exploration					1643:1653	the exploration	1639:1653	the exploration of the thermophily mechanism of thermophilic fungi	1639:1704	This is the novel study to reveal thermophilic fungi's physiological response to high-temperature adaptation using omics analysis, facilitating the exploration of the thermophily mechanism of thermophilic fungi.
34163440	7	80	theme	pathway	1237:1243	arg1	types					1187:1191	various types	1179:1191	various types of N-glycan biosynthesis, mRNA surveillance pathway, and protein processing in the endoplasmic reticulum	1179:1296	The differential glycoproteins were mainly involved in various types of N-glycan biosynthesis, mRNA surveillance pathway, and protein processing in the endoplasmic reticulum.
32808408	9	0	theme	beneficial	1764:1773	arg1	changes					1775:1781	overall beneficial changes	1756:1781	overall beneficial changes in human colonic microbiota	1756:1809	CONCLUSION AB and GB could be novel prebiotic ingredients because they can stimulate the growth and metabolism of probiotics and induce overall beneficial changes in human colonic microbiota.
32808408	0	1	theme	beneficial	167:176	arg1	changes					178:184	beneficial changes	167:184	beneficial changes in colonic microbiota	167:206	Acerola (Malpighia glabra L.) and guava (Psidium guayaba L.) industrial processing by-products stimulate probiotic Lactobacillus and Bifidobacterium growth and induce beneficial changes in colonic microbiota.
32808408	1	2	theme	industrial	260:269	arg1	processing					271:280	industrial processing	260:280	industrial processing of acerola (Malpighia glabra L.; AB) and guava (Psidium guajava L.; GB) fruit	260:358	AIMS This study evaluated whether by-products from industrial processing of acerola (Malpighia glabra L.; AB) and guava (Psidium guajava L.; GB) fruit may stimulate the growth and metabolism of probiotic Lactobacillus and Bifidobacterium and induce changes in human colonic microbiota.
32808408	11	3	theme	compounds	2175:2183	arg1	sources					2152:2158	sources	2152:2158	sources of added-value compounds	2152:2183	These results characterize AB and GB as potential prebiotic ingredients and fruit processing by-products as sources of added-value compounds.
32808408	11	3	theme	compounds	2175:2183	arg1	GB					2078:2079	GB	2078:2079	GB	2078:2079	These results characterize AB and GB as potential prebiotic ingredients and fruit processing by-products as sources of added-value compounds.
32808408	11	3	theme	compounds	2175:2183	arg1	AB					2071:2072	AB	2071:2072	AB	2071:2072	These results characterize AB and GB as potential prebiotic ingredients and fruit processing by-products as sources of added-value compounds.
32808408	1	4	theme	Lactobacillus	413:425	arg1	growth					378:383	growth	378:383	growth	378:383	AIMS This study evaluated whether by-products from industrial processing of acerola (Malpighia glabra L.; AB) and guava (Psidium guajava L.; GB) fruit may stimulate the growth and metabolism of probiotic Lactobacillus and Bifidobacterium and induce changes in human colonic microbiota.
32808408	1	4	theme	Lactobacillus	413:425	arg1	metabolism					389:398	metabolism	389:398	metabolism	389:398	AIMS This study evaluated whether by-products from industrial processing of acerola (Malpighia glabra L.; AB) and guava (Psidium guajava L.; GB) fruit may stimulate the growth and metabolism of probiotic Lactobacillus and Bifidobacterium and induce changes in human colonic microbiota.
32808408	3	5	theme	lactis	700:705	arg1	BB-12					707:711	lactis BB-12	700:711	lactis BB-12	700:711	lactis BB-12 was evaluated.
32808408	1	6	theme	Bifidobacterium	431:445	arg1	growth					378:383	growth	378:383	growth	378:383	AIMS This study evaluated whether by-products from industrial processing of acerola (Malpighia glabra L.; AB) and guava (Psidium guajava L.; GB) fruit may stimulate the growth and metabolism of probiotic Lactobacillus and Bifidobacterium and induce changes in human colonic microbiota.
32808408	1	6	theme	Bifidobacterium	431:445	arg1	metabolism					389:398	metabolism	389:398	metabolism	389:398	AIMS This study evaluated whether by-products from industrial processing of acerola (Malpighia glabra L.; AB) and guava (Psidium guajava L.; GB) fruit may stimulate the growth and metabolism of probiotic Lactobacillus and Bifidobacterium and induce changes in human colonic microbiota.
32808408	10	7	from	alterations	1944:1954	arg1	composition					1984:1994	human colonic microbiota composition	1959:1994	human colonic microbiota composition	1959:1994	SIGNIFICANCE AND IMPACT OF THE STUDY AB and GB stimulated the growth and metabolism of probiotics, in addition to induce beneficial alterations in human colonic microbiota composition and increase short-chain fatty acid production.
32808408	2	8	theme	ad	589:590	arg1	metabolism					592:601	the growth ad metabolism	578:601	the growth ad metabolism of Lactobacillus acidophilus LA-05, Lactobacillus casei L-26 and Bifidobacterium animalis subsp	578:697	METHODS AND RESULTS The ability of non-digested and digested AB or GB to stimulate the growth ad metabolism of Lactobacillus acidophilus LA-05, Lactobacillus casei L-26 and Bifidobacterium animalis subsp.
32808408	7	9	with	media	1156:1160	arg1	GB					1200:1201	GB	1200:1201	GB	1200:1201	Cultivation of probiotics in media with non-digested and digested AB and GB resulted in decreased pH, increased organic acid production and sugar consumption over time.
32808408	7	9	with	media	1156:1160	arg1	AB					1193:1194	AB	1193:1194	AB	1193:1194	Cultivation of probiotics in media with non-digested and digested AB and GB resulted in decreased pH, increased organic acid production and sugar consumption over time.
32808408	4	10	theme	colonic	871:877	arg1	system					892:897	an in vitro colonic fermentation system	859:897	an in vitro colonic fermentation system	859:897	Changes in populations of distinct bacterial groups of human colonic microbiota induced by digested AB and GB were evaluated using an in vitro colonic fermentation system.
32808408	10	11	theme	acid	2027:2030	arg1	production					2032:2041	short-chain fatty acid production	2009:2041	short-chain fatty acid production	2009:2041	SIGNIFICANCE AND IMPACT OF THE STUDY AB and GB stimulated the growth and metabolism of probiotics, in addition to induce beneficial alterations in human colonic microbiota composition and increase short-chain fatty acid production.
32808408	2	12	theme	non-digested	530:541	arg1	AB					556:557	AB	556:557	AB	556:557	METHODS AND RESULTS The ability of non-digested and digested AB or GB to stimulate the growth ad metabolism of Lactobacillus acidophilus LA-05, Lactobacillus casei L-26 and Bifidobacterium animalis subsp.
32808408	10	13	theme	short-chain	2009:2019	arg1	acid					2027:2030	short-chain fatty acid	2009:2030	short-chain fatty acid production	2009:2041	SIGNIFICANCE AND IMPACT OF THE STUDY AB and GB stimulated the growth and metabolism of probiotics, in addition to induce beneficial alterations in human colonic microbiota composition and increase short-chain fatty acid production.
32808408	11	14	theme	potential	2084:2092	arg1	GB					2078:2079	GB	2078:2079	GB	2078:2079	These results characterize AB and GB as potential prebiotic ingredients and fruit processing by-products as sources of added-value compounds.
32808408	11	14	theme	potential	2084:2092	arg1	AB					2071:2072	AB	2071:2072	AB	2071:2072	These results characterize AB and GB as potential prebiotic ingredients and fruit processing by-products as sources of added-value compounds.
32808408	11	14	theme	potential	2084:2092	arg1	ingredients					2104:2114	potential prebiotic ingredients	2084:2114	potential prebiotic ingredients	2084:2114	These results characterize AB and GB as potential prebiotic ingredients and fruit processing by-products as sources of added-value compounds.
32808408	8	15	theme	populations	1484:1494	arg1	abundance					1352:1360	abundance	1352:1360	abundance of Bifidobacterium spp., Lactobacillus-Enterococcus, Eubacterium rectall-Clostridium coccoides and Bacteroides-Provotella populations	1352:1494	Digested AB and GB caused overall beneficial changes in abundance of Bifidobacterium spp., Lactobacillus-Enterococcus, Eubacterium rectall-Clostridium coccoides and Bacteroides-Provotella populations, besides to decrease the pH and increase the short-chain fatty acid production during a 24-h in vitro colonic fermentation.
32808408	6	16	with	media	1007:1011	arg1	AB					1080:1081	non-digested and digested AB	1054:1081	non-digested and digested AB	1054:1081	No difference among counts of probiotics in media with glucose, fructooligosaccharides and non-digested and digested AB and GB was found during a 48-h cultivation.
32808408	6	16	with	media	1007:1011	arg1	fructooligosaccharides					1027:1048	fructooligosaccharides	1027:1048	fructooligosaccharides	1027:1048	No difference among counts of probiotics in media with glucose, fructooligosaccharides and non-digested and digested AB and GB was found during a 48-h cultivation.
32808408	6	16	with	media	1007:1011	arg1	glucose					1018:1024	glucose	1018:1024	glucose	1018:1024	No difference among counts of probiotics in media with glucose, fructooligosaccharides and non-digested and digested AB and GB was found during a 48-h cultivation.
32808408	4	17	theme	fermentation	879:890	arg1	system					892:897	an in vitro colonic fermentation system	859:897	an in vitro colonic fermentation system	859:897	Changes in populations of distinct bacterial groups of human colonic microbiota induced by digested AB and GB were evaluated using an in vitro colonic fermentation system.
32808408	2	18	theme	subsp	693:697	arg1	metabolism					592:601	the growth ad metabolism	578:601	the growth ad metabolism of Lactobacillus acidophilus LA-05, Lactobacillus casei L-26 and Bifidobacterium animalis subsp	578:697	METHODS AND RESULTS The ability of non-digested and digested AB or GB to stimulate the growth ad metabolism of Lactobacillus acidophilus LA-05, Lactobacillus casei L-26 and Bifidobacterium animalis subsp.
32808408	0	19	theme	Bifidobacterium	133:147	arg1	growth					149:154	probiotic Lactobacillus and Bifidobacterium growth	105:154	growth	149:154	Acerola (Malpighia glabra L.) and guava (Psidium guayaba L.) industrial processing by-products stimulate probiotic Lactobacillus and Bifidobacterium growth and induce beneficial changes in colonic microbiota.
32808408	10	20	theme	STUDY	1843:1847	arg1	IMPACT					1829:1834	IMPACT	1829:1834	IMPACT	1829:1834	SIGNIFICANCE AND IMPACT OF THE STUDY AB and GB stimulated the growth and metabolism of probiotics, in addition to induce beneficial alterations in human colonic microbiota composition and increase short-chain fatty acid production.
32808408	10	20	theme	STUDY	1843:1847	arg1	SIGNIFICANCE					1812:1823	SIGNIFICANCE	1812:1823	SIGNIFICANCE	1812:1823	SIGNIFICANCE AND IMPACT OF THE STUDY AB and GB stimulated the growth and metabolism of probiotics, in addition to induce beneficial alterations in human colonic microbiota composition and increase short-chain fatty acid production.
32808408	2	21	theme	digested	547:554	arg1	AB					556:557	AB	556:557	AB	556:557	METHODS AND RESULTS The ability of non-digested and digested AB or GB to stimulate the growth ad metabolism of Lactobacillus acidophilus LA-05, Lactobacillus casei L-26 and Bifidobacterium animalis subsp.
32808408	8	22	theme	Digested	1296:1303	arg1	AB					1305:1306	Digested AB	1296:1306	Digested AB	1296:1306	Digested AB and GB caused overall beneficial changes in abundance of Bifidobacterium spp., Lactobacillus-Enterococcus, Eubacterium rectall-Clostridium coccoides and Bacteroides-Provotella populations, besides to decrease the pH and increase the short-chain fatty acid production during a 24-h in vitro colonic fermentation.
32808408	8	23	dep	in	1589:1590	arg1	vitro					1592:1596	vitro	1592:1596	vitro	1592:1596	Digested AB and GB caused overall beneficial changes in abundance of Bifidobacterium spp., Lactobacillus-Enterococcus, Eubacterium rectall-Clostridium coccoides and Bacteroides-Provotella populations, besides to decrease the pH and increase the short-chain fatty acid production during a 24-h in vitro colonic fermentation.
32808408	9	24	theme	colonic	1792:1798	arg1	microbiota					1800:1809	human colonic microbiota	1786:1809	human colonic microbiota	1786:1809	CONCLUSION AB and GB could be novel prebiotic ingredients because they can stimulate the growth and metabolism of probiotics and induce overall beneficial changes in human colonic microbiota.
32808408	9	25	dep	CONCLUSION	1620:1629	arg1	GB					1638:1639	GB	1638:1639	GB	1638:1639	CONCLUSION AB and GB could be novel prebiotic ingredients because they can stimulate the growth and metabolism of probiotics and induce overall beneficial changes in human colonic microbiota.
32808408	9	25	dep	CONCLUSION	1620:1629	arg1	AB					1631:1632	AB	1631:1632	AB	1631:1632	CONCLUSION AB and GB could be novel prebiotic ingredients because they can stimulate the growth and metabolism of probiotics and induce overall beneficial changes in human colonic microbiota.
32808408	9	26	from	changes	1775:1781	arg1	microbiota					1800:1809	human colonic microbiota	1786:1809	human colonic microbiota	1786:1809	CONCLUSION AB and GB could be novel prebiotic ingredients because they can stimulate the growth and metabolism of probiotics and induce overall beneficial changes in human colonic microbiota.
32808408	8	27	theme	colonic	1598:1604	arg1	fermentation					1606:1617	a 24-h in vitro colonic fermentation	1582:1617	a 24-h in vitro colonic fermentation	1582:1617	Digested AB and GB caused overall beneficial changes in abundance of Bifidobacterium spp., Lactobacillus-Enterococcus, Eubacterium rectall-Clostridium coccoides and Bacteroides-Provotella populations, besides to decrease the pH and increase the short-chain fatty acid production during a 24-h in vitro colonic fermentation.
32808408	2	28	theme	GB	562:563	arg1	ability					519:525	The ability	515:525	The ability of non-digested and digested AB or GB	515:563	METHODS AND RESULTS The ability of non-digested and digested AB or GB to stimulate the growth ad metabolism of Lactobacillus acidophilus LA-05, Lactobacillus casei L-26 and Bifidobacterium animalis subsp.
32808408	8	29	theme	short-chain	1541:1551	arg1	acid					1559:1562	the short-chain fatty acid	1537:1562	the short-chain fatty acid production	1537:1573	Digested AB and GB caused overall beneficial changes in abundance of Bifidobacterium spp., Lactobacillus-Enterococcus, Eubacterium rectall-Clostridium coccoides and Bacteroides-Provotella populations, besides to decrease the pH and increase the short-chain fatty acid production during a 24-h in vitro colonic fermentation.
32808408	4	30	theme	distinct	754:761	arg1	microbiota					797:806	human colonic microbiota	783:806	human colonic microbiota	783:806	Changes in populations of distinct bacterial groups of human colonic microbiota induced by digested AB and GB were evaluated using an in vitro colonic fermentation system.
32808408	4	30	theme	distinct	754:761	arg1	groups					773:778	distinct bacterial groups	754:778	distinct bacterial groups of human colonic microbiota	754:806	Changes in populations of distinct bacterial groups of human colonic microbiota induced by digested AB and GB were evaluated using an in vitro colonic fermentation system.
32808408	8	31	theme	acid	1559:1562	arg1	production					1564:1573	the short-chain fatty acid production	1537:1573	the short-chain fatty acid production	1537:1573	Digested AB and GB caused overall beneficial changes in abundance of Bifidobacterium spp., Lactobacillus-Enterococcus, Eubacterium rectall-Clostridium coccoides and Bacteroides-Provotella populations, besides to decrease the pH and increase the short-chain fatty acid production during a 24-h in vitro colonic fermentation.
32808408	4	32	theme	groups	773:778	arg1	populations					739:749	populations	739:749	populations of distinct bacterial groups of human colonic microbiota	739:806	Changes in populations of distinct bacterial groups of human colonic microbiota induced by digested AB and GB were evaluated using an in vitro colonic fermentation system.
32808408	0	33	theme	processing	72:81	arg1	Psidium					41:47	Psidium	41:47	Psidium	41:47	Acerola (Malpighia glabra L.) and guava (Psidium guayaba L.) industrial processing by-products stimulate probiotic Lactobacillus and Bifidobacterium growth and induce beneficial changes in colonic microbiota.
32808408	0	33	theme	processing	72:81	arg1	by-products					83:93	Acerola (Malpighia glabra L.) and guava (Psidium guayaba L.) industrial processing by-products	0:93	by-products	83:93	Acerola (Malpighia glabra L.) and guava (Psidium guayaba L.) industrial processing by-products stimulate probiotic Lactobacillus and Bifidobacterium growth and induce beneficial changes in colonic microbiota.
32808408	10	34	theme	microbiota	1973:1982	arg1	composition					1984:1994	human colonic microbiota composition	1959:1994	human colonic microbiota composition	1959:1994	SIGNIFICANCE AND IMPACT OF THE STUDY AB and GB stimulated the growth and metabolism of probiotics, in addition to induce beneficial alterations in human colonic microbiota composition and increase short-chain fatty acid production.
32808408	1	35	theme	acerola	285:291	arg1	processing					271:280	industrial processing	260:280	industrial processing of acerola (Malpighia glabra L.; AB) and guava (Psidium guajava L.; GB) fruit	260:358	AIMS This study evaluated whether by-products from industrial processing of acerola (Malpighia glabra L.; AB) and guava (Psidium guajava L.; GB) fruit may stimulate the growth and metabolism of probiotic Lactobacillus and Bifidobacterium and induce changes in human colonic microbiota.
32808408	4	36	from	Changes	728:734	arg1	populations					739:749	populations	739:749	populations of distinct bacterial groups of human colonic microbiota	739:806	Changes in populations of distinct bacterial groups of human colonic microbiota induced by digested AB and GB were evaluated using an in vitro colonic fermentation system.
32808408	8	37	theme	24-h	1584:1587	arg1	fermentation					1606:1617	a 24-h in vitro colonic fermentation	1582:1617	a 24-h in vitro colonic fermentation	1582:1617	Digested AB and GB caused overall beneficial changes in abundance of Bifidobacterium spp., Lactobacillus-Enterococcus, Eubacterium rectall-Clostridium coccoides and Bacteroides-Provotella populations, besides to decrease the pH and increase the short-chain fatty acid production during a 24-h in vitro colonic fermentation.
32808408	7	38	dep	resulted	1203:1210	arg1	increased					1229:1237	increased	1229:1237	increased organic acid production and sugar consumption over time	1229:1293	Cultivation of probiotics in media with non-digested and digested AB and GB resulted in decreased pH, increased organic acid production and sugar consumption over time.
32808408	8	39	theme	spp.	1381:1384	arg1	abundance					1352:1360	abundance	1352:1360	abundance of Bifidobacterium spp., Lactobacillus-Enterococcus, Eubacterium rectall-Clostridium coccoides and Bacteroides-Provotella populations	1352:1494	Digested AB and GB caused overall beneficial changes in abundance of Bifidobacterium spp., Lactobacillus-Enterococcus, Eubacterium rectall-Clostridium coccoides and Bacteroides-Provotella populations, besides to decrease the pH and increase the short-chain fatty acid production during a 24-h in vitro colonic fermentation.
32808408	5	40	theme	probiotic	945:953	arg1	growth					955:960	probiotic growth	945:960	probiotic growth	945:960	Non-digested and digested AB and GB favoured probiotic growth.
32808408	6	41	from	difference	966:975	arg1	media					1007:1011	media	1007:1011	media with glucose, fructooligosaccharides and non-digested and digested AB	1007:1081	No difference among counts of probiotics in media with glucose, fructooligosaccharides and non-digested and digested AB and GB was found during a 48-h cultivation.
32808408	10	42	theme	probiotics	1899:1908	arg1	metabolism					1885:1894	metabolism	1885:1894	metabolism	1885:1894	SIGNIFICANCE AND IMPACT OF THE STUDY AB and GB stimulated the growth and metabolism of probiotics, in addition to induce beneficial alterations in human colonic microbiota composition and increase short-chain fatty acid production.
32808408	10	42	theme	probiotics	1899:1908	arg1	growth					1874:1879	growth	1874:1879	growth	1874:1879	SIGNIFICANCE AND IMPACT OF THE STUDY AB and GB stimulated the growth and metabolism of probiotics, in addition to induce beneficial alterations in human colonic microbiota composition and increase short-chain fatty acid production.
32808408	8	43	theme	Lactobacillus-Enterococcus	1387:1412	arg1	abundance					1352:1360	abundance	1352:1360	abundance of Bifidobacterium spp., Lactobacillus-Enterococcus, Eubacterium rectall-Clostridium coccoides and Bacteroides-Provotella populations	1352:1494	Digested AB and GB caused overall beneficial changes in abundance of Bifidobacterium spp., Lactobacillus-Enterococcus, Eubacterium rectall-Clostridium coccoides and Bacteroides-Provotella populations, besides to decrease the pH and increase the short-chain fatty acid production during a 24-h in vitro colonic fermentation.
32808408	4	44	dep	in	862:863	arg1	vitro					865:869	vitro	865:869	vitro	865:869	Changes in populations of distinct bacterial groups of human colonic microbiota induced by digested AB and GB were evaluated using an in vitro colonic fermentation system.
32808408	1	45	theme	human	469:473	arg1	microbiota					483:492	human colonic microbiota	469:492	human colonic microbiota	469:492	AIMS This study evaluated whether by-products from industrial processing of acerola (Malpighia glabra L.; AB) and guava (Psidium guajava L.; GB) fruit may stimulate the growth and metabolism of probiotic Lactobacillus and Bifidobacterium and induce changes in human colonic microbiota.
32808408	1	46	from	changes	458:464	arg1	microbiota					483:492	human colonic microbiota	469:492	human colonic microbiota	469:492	AIMS This study evaluated whether by-products from industrial processing of acerola (Malpighia glabra L.; AB) and guava (Psidium guajava L.; GB) fruit may stimulate the growth and metabolism of probiotic Lactobacillus and Bifidobacterium and induce changes in human colonic microbiota.
32808408	1	47	dep	L.	346:347	arg1	GB					350:351	GB	350:351	GB	350:351	AIMS This study evaluated whether by-products from industrial processing of acerola (Malpighia glabra L.; AB) and guava (Psidium guajava L.; GB) fruit may stimulate the growth and metabolism of probiotic Lactobacillus and Bifidobacterium and induce changes in human colonic microbiota.
32808408	1	48	dep	AIMS	209:212	arg1	evaluated					225:233	evaluated	225:233	evaluated whether by-products from industrial processing of acerola (Malpighia glabra L.; AB) and guava (Psidium guajava L.; GB) fruit may stimulate the growth and metabolism of probiotic Lactobacillus and Bifidobacterium and induce changes in human colonic microbiota	225:492	AIMS This study evaluated whether by-products from industrial processing of acerola (Malpighia glabra L.; AB) and guava (Psidium guajava L.; GB) fruit may stimulate the growth and metabolism of probiotic Lactobacillus and Bifidobacterium and induce changes in human colonic microbiota.
32808408	8	49	theme	coccoides	1447:1455	arg1	abundance					1352:1360	abundance	1352:1360	abundance of Bifidobacterium spp., Lactobacillus-Enterococcus, Eubacterium rectall-Clostridium coccoides and Bacteroides-Provotella populations	1352:1494	Digested AB and GB caused overall beneficial changes in abundance of Bifidobacterium spp., Lactobacillus-Enterococcus, Eubacterium rectall-Clostridium coccoides and Bacteroides-Provotella populations, besides to decrease the pH and increase the short-chain fatty acid production during a 24-h in vitro colonic fermentation.
32808408	8	50	from	changes	1341:1347	arg1	abundance					1352:1360	abundance	1352:1360	abundance of Bifidobacterium spp., Lactobacillus-Enterococcus, Eubacterium rectall-Clostridium coccoides and Bacteroides-Provotella populations	1352:1494	Digested AB and GB caused overall beneficial changes in abundance of Bifidobacterium spp., Lactobacillus-Enterococcus, Eubacterium rectall-Clostridium coccoides and Bacteroides-Provotella populations, besides to decrease the pH and increase the short-chain fatty acid production during a 24-h in vitro colonic fermentation.
32808408	9	51	theme	prebiotic	1656:1664	arg1	ingredients					1666:1676	novel prebiotic ingredients	1650:1676	novel prebiotic ingredients	1650:1676	CONCLUSION AB and GB could be novel prebiotic ingredients because they can stimulate the growth and metabolism of probiotics and induce overall beneficial changes in human colonic microbiota.
32808408	8	52	theme	Bacteroides-Provotella	1461:1482	arg1	populations					1484:1494	Bacteroides-Provotella populations	1461:1494	Bacteroides-Provotella populations	1461:1494	Digested AB and GB caused overall beneficial changes in abundance of Bifidobacterium spp., Lactobacillus-Enterococcus, Eubacterium rectall-Clostridium coccoides and Bacteroides-Provotella populations, besides to decrease the pH and increase the short-chain fatty acid production during a 24-h in vitro colonic fermentation.
32808408	6	53	from	GB	1087:1088	arg1	media					1007:1011	media	1007:1011	media with glucose, fructooligosaccharides and non-digested and digested AB	1007:1081	No difference among counts of probiotics in media with glucose, fructooligosaccharides and non-digested and digested AB and GB was found during a 48-h cultivation.
32808408	7	54	theme	acid	1247:1250	arg1	production					1252:1261	organic acid production	1239:1261	organic acid production	1239:1261	Cultivation of probiotics in media with non-digested and digested AB and GB resulted in decreased pH, increased organic acid production and sugar consumption over time.
32808408	10	55	theme	beneficial	1933:1942	arg1	alterations					1944:1954	beneficial alterations	1933:1954	beneficial alterations in human colonic microbiota composition	1933:1994	SIGNIFICANCE AND IMPACT OF THE STUDY AB and GB stimulated the growth and metabolism of probiotics, in addition to induce beneficial alterations in human colonic microbiota composition and increase short-chain fatty acid production.
32808408	0	56	theme	colonic	189:195	arg1	microbiota					197:206	colonic microbiota	189:206	colonic microbiota	189:206	Acerola (Malpighia glabra L.) and guava (Psidium guayaba L.) industrial processing by-products stimulate probiotic Lactobacillus and Bifidobacterium growth and induce beneficial changes in colonic microbiota.
32808408	1	57	theme	probiotic	403:411	arg1	Lactobacillus					413:425	probiotic Lactobacillus	403:425	probiotic Lactobacillus	403:425	AIMS This study evaluated whether by-products from industrial processing of acerola (Malpighia glabra L.; AB) and guava (Psidium guajava L.; GB) fruit may stimulate the growth and metabolism of probiotic Lactobacillus and Bifidobacterium and induce changes in human colonic microbiota.
32808408	4	58	theme	in	862:863	arg1	system					892:897	an in vitro colonic fermentation system	859:897	an in vitro colonic fermentation system	859:897	Changes in populations of distinct bacterial groups of human colonic microbiota induced by digested AB and GB were evaluated using an in vitro colonic fermentation system.
32808408	10	59	theme	colonic	1965:1971	arg1	composition					1984:1994	human colonic microbiota composition	1959:1994	human colonic microbiota composition	1959:1994	SIGNIFICANCE AND IMPACT OF THE STUDY AB and GB stimulated the growth and metabolism of probiotics, in addition to induce beneficial alterations in human colonic microbiota composition and increase short-chain fatty acid production.
32808408	11	60	theme	prebiotic	2094:2102	arg1	GB					2078:2079	GB	2078:2079	GB	2078:2079	These results characterize AB and GB as potential prebiotic ingredients and fruit processing by-products as sources of added-value compounds.
32808408	11	60	theme	prebiotic	2094:2102	arg1	AB					2071:2072	AB	2071:2072	AB	2071:2072	These results characterize AB and GB as potential prebiotic ingredients and fruit processing by-products as sources of added-value compounds.
32808408	11	60	theme	prebiotic	2094:2102	arg1	ingredients					2104:2114	potential prebiotic ingredients	2084:2114	potential prebiotic ingredients	2084:2114	These results characterize AB and GB as potential prebiotic ingredients and fruit processing by-products as sources of added-value compounds.
32808408	8	61	theme	fatty	1553:1557	arg1	acid					1559:1562	the short-chain fatty acid	1537:1562	the short-chain fatty acid production	1537:1573	Digested AB and GB caused overall beneficial changes in abundance of Bifidobacterium spp., Lactobacillus-Enterococcus, Eubacterium rectall-Clostridium coccoides and Bacteroides-Provotella populations, besides to decrease the pH and increase the short-chain fatty acid production during a 24-h in vitro colonic fermentation.
32808408	2	62	theme	growth	582:587	arg1	metabolism					592:601	the growth ad metabolism	578:601	the growth ad metabolism of Lactobacillus acidophilus LA-05, Lactobacillus casei L-26 and Bifidobacterium animalis subsp	578:697	METHODS AND RESULTS The ability of non-digested and digested AB or GB to stimulate the growth ad metabolism of Lactobacillus acidophilus LA-05, Lactobacillus casei L-26 and Bifidobacterium animalis subsp.
32808408	10	63	theme	fatty	2021:2025	arg1	acid					2027:2030	short-chain fatty acid	2009:2030	short-chain fatty acid production	2009:2041	SIGNIFICANCE AND IMPACT OF THE STUDY AB and GB stimulated the growth and metabolism of probiotics, in addition to induce beneficial alterations in human colonic microbiota composition and increase short-chain fatty acid production.
32808408	0	64	theme	probiotic	105:113	arg1	Lactobacillus					115:127	probiotic Lactobacillus and Bifidobacterium growth	105:154	Lactobacillus	115:127	Acerola (Malpighia glabra L.) and guava (Psidium guayaba L.) industrial processing by-products stimulate probiotic Lactobacillus and Bifidobacterium growth and induce beneficial changes in colonic microbiota.
32808408	10	65	dep	STUDY	1843:1847	arg1	GB					1856:1857	GB	1856:1857	GB	1856:1857	SIGNIFICANCE AND IMPACT OF THE STUDY AB and GB stimulated the growth and metabolism of probiotics, in addition to induce beneficial alterations in human colonic microbiota composition and increase short-chain fatty acid production.
32808408	10	65	dep	STUDY	1843:1847	arg1	AB					1849:1850	AB	1849:1850	AB	1849:1850	SIGNIFICANCE AND IMPACT OF THE STUDY AB and GB stimulated the growth and metabolism of probiotics, in addition to induce beneficial alterations in human colonic microbiota composition and increase short-chain fatty acid production.
32808408	9	66	theme	overall	1756:1762	arg1	changes					1775:1781	overall beneficial changes	1756:1781	overall beneficial changes in human colonic microbiota	1756:1809	CONCLUSION AB and GB could be novel prebiotic ingredients because they can stimulate the growth and metabolism of probiotics and induce overall beneficial changes in human colonic microbiota.
32808408	11	67	theme	added-value	2163:2173	arg1	compounds					2175:2183	added-value compounds	2163:2183	added-value compounds	2163:2183	These results characterize AB and GB as potential prebiotic ingredients and fruit processing by-products as sources of added-value compounds.
32808408	5	68	theme	Non-digested	900:911	arg1	AB					926:927	AB	926:927	AB	926:927	Non-digested and digested AB and GB favoured probiotic growth.
32808408	1	69	from	processing	271:280	arg1	by-products					243:253	by-products	243:253	by-products from industrial processing of acerola (Malpighia glabra L.; AB) and guava (Psidium guajava L.; GB) fruit	243:358	AIMS This study evaluated whether by-products from industrial processing of acerola (Malpighia glabra L.; AB) and guava (Psidium guajava L.; GB) fruit may stimulate the growth and metabolism of probiotic Lactobacillus and Bifidobacterium and induce changes in human colonic microbiota.
32808408	2	70	theme	AB	556:557	arg1	ability					519:525	The ability	515:525	The ability of non-digested and digested AB or GB	515:563	METHODS AND RESULTS The ability of non-digested and digested AB or GB to stimulate the growth ad metabolism of Lactobacillus acidophilus LA-05, Lactobacillus casei L-26 and Bifidobacterium animalis subsp.
32808408	2	71	theme	animalis	684:691	arg1	subsp					693:697	Lactobacillus acidophilus LA-05, Lactobacillus casei L-26 and Bifidobacterium animalis subsp	606:697	Lactobacillus acidophilus LA-05, Lactobacillus casei L-26 and Bifidobacterium animalis subsp	606:697	METHODS AND RESULTS The ability of non-digested and digested AB or GB to stimulate the growth ad metabolism of Lactobacillus acidophilus LA-05, Lactobacillus casei L-26 and Bifidobacterium animalis subsp.
32808408	4	72	theme	human	783:787	arg1	microbiota					797:806	human colonic microbiota	783:806	human colonic microbiota	783:806	Changes in populations of distinct bacterial groups of human colonic microbiota induced by digested AB and GB were evaluated using an in vitro colonic fermentation system.
32808408	5	73	theme	digested	917:924	arg1	AB					926:927	AB	926:927	AB	926:927	Non-digested and digested AB and GB favoured probiotic growth.
32808408	9	74	theme	human	1786:1790	arg1	microbiota					1800:1809	human colonic microbiota	1786:1809	human colonic microbiota	1786:1809	CONCLUSION AB and GB could be novel prebiotic ingredients because they can stimulate the growth and metabolism of probiotics and induce overall beneficial changes in human colonic microbiota.
32808408	8	75	theme	beneficial	1330:1339	arg1	changes					1341:1347	overall beneficial changes	1322:1347	overall beneficial changes in abundance of Bifidobacterium spp., Lactobacillus-Enterococcus, Eubacterium rectall-Clostridium coccoides and Bacteroides-Provotella populations	1322:1494	Digested AB and GB caused overall beneficial changes in abundance of Bifidobacterium spp., Lactobacillus-Enterococcus, Eubacterium rectall-Clostridium coccoides and Bacteroides-Provotella populations, besides to decrease the pH and increase the short-chain fatty acid production during a 24-h in vitro colonic fermentation.
32808408	7	76	theme	decreased	1215:1223	arg1	pH					1225:1226	decreased pH	1215:1226	decreased pH	1215:1226	Cultivation of probiotics in media with non-digested and digested AB and GB resulted in decreased pH, increased organic acid production and sugar consumption over time.
32808408	11	77	theme	processing	2126:2135	arg1	by-products					2137:2147	fruit processing by-products	2120:2147	fruit processing by-products	2120:2147	These results characterize AB and GB as potential prebiotic ingredients and fruit processing by-products as sources of added-value compounds.
32808408	11	77	theme	processing	2126:2135	arg1	GB					2078:2079	GB	2078:2079	GB	2078:2079	These results characterize AB and GB as potential prebiotic ingredients and fruit processing by-products as sources of added-value compounds.
32808408	11	77	theme	processing	2126:2135	arg1	AB					2071:2072	AB	2071:2072	AB	2071:2072	These results characterize AB and GB as potential prebiotic ingredients and fruit processing by-products as sources of added-value compounds.
32808408	7	78	from	Cultivation	1127:1137	arg1	media					1156:1160	media	1156:1160	media with non-digested and digested AB and GB	1156:1201	Cultivation of probiotics in media with non-digested and digested AB and GB resulted in decreased pH, increased organic acid production and sugar consumption over time.
32808408	1	79	theme	guava	323:327	arg1	processing					271:280	industrial processing	260:280	industrial processing of acerola (Malpighia glabra L.; AB) and guava (Psidium guajava L.; GB) fruit	260:358	AIMS This study evaluated whether by-products from industrial processing of acerola (Malpighia glabra L.; AB) and guava (Psidium guajava L.; GB) fruit may stimulate the growth and metabolism of probiotic Lactobacillus and Bifidobacterium and induce changes in human colonic microbiota.
32808408	6	80	theme	probiotics	993:1002	arg1	counts					983:988	counts	983:988	counts of probiotics	983:1002	No difference among counts of probiotics in media with glucose, fructooligosaccharides and non-digested and digested AB and GB was found during a 48-h cultivation.
32808408	1	81	dep	acerola	285:291	arg1	fruit					354:358	fruit	354:358	fruit	354:358	AIMS This study evaluated whether by-products from industrial processing of acerola (Malpighia glabra L.; AB) and guava (Psidium guajava L.; GB) fruit may stimulate the growth and metabolism of probiotic Lactobacillus and Bifidobacterium and induce changes in human colonic microbiota.
32808408	0	82	theme	industrial	61:70	arg1	Psidium					41:47	Psidium	41:47	Psidium	41:47	Acerola (Malpighia glabra L.) and guava (Psidium guayaba L.) industrial processing by-products stimulate probiotic Lactobacillus and Bifidobacterium growth and induce beneficial changes in colonic microbiota.
32808408	0	82	theme	industrial	61:70	arg1	by-products					83:93	Acerola (Malpighia glabra L.) and guava (Psidium guayaba L.) industrial processing by-products	0:93	by-products	83:93	Acerola (Malpighia glabra L.) and guava (Psidium guayaba L.) industrial processing by-products stimulate probiotic Lactobacillus and Bifidobacterium growth and induce beneficial changes in colonic microbiota.
32808408	0	83	dep	Malpighia	9:17	arg1	L.					26:27	Malpighia glabra L.	9:27	Malpighia glabra L.	9:27	Acerola (Malpighia glabra L.) and guava (Psidium guayaba L.) industrial processing by-products stimulate probiotic Lactobacillus and Bifidobacterium growth and induce beneficial changes in colonic microbiota.
32808408	2	84	theme	acidophilus	620:630	arg1	subsp					693:697	Lactobacillus acidophilus LA-05, Lactobacillus casei L-26 and Bifidobacterium animalis subsp	606:697	Lactobacillus acidophilus LA-05, Lactobacillus casei L-26 and Bifidobacterium animalis subsp	606:697	METHODS AND RESULTS The ability of non-digested and digested AB or GB to stimulate the growth ad metabolism of Lactobacillus acidophilus LA-05, Lactobacillus casei L-26 and Bifidobacterium animalis subsp.
32808408	8	85	theme	overall	1322:1328	arg1	changes					1341:1347	overall beneficial changes	1322:1347	overall beneficial changes in abundance of Bifidobacterium spp., Lactobacillus-Enterococcus, Eubacterium rectall-Clostridium coccoides and Bacteroides-Provotella populations	1322:1494	Digested AB and GB caused overall beneficial changes in abundance of Bifidobacterium spp., Lactobacillus-Enterococcus, Eubacterium rectall-Clostridium coccoides and Bacteroides-Provotella populations, besides to decrease the pH and increase the short-chain fatty acid production during a 24-h in vitro colonic fermentation.
32808408	7	86	theme	digested	1184:1191	arg1	AB					1193:1194	AB	1193:1194	AB	1193:1194	Cultivation of probiotics in media with non-digested and digested AB and GB resulted in decreased pH, increased organic acid production and sugar consumption over time.
32808408	1	87	dep	L.	311:312	arg1	AB					315:316	AB	315:316	AB	315:316	AIMS This study evaluated whether by-products from industrial processing of acerola (Malpighia glabra L.; AB) and guava (Psidium guajava L.; GB) fruit may stimulate the growth and metabolism of probiotic Lactobacillus and Bifidobacterium and induce changes in human colonic microbiota.
32808408	4	88	theme	bacterial	763:771	arg1	microbiota					797:806	human colonic microbiota	783:806	human colonic microbiota	783:806	Changes in populations of distinct bacterial groups of human colonic microbiota induced by digested AB and GB were evaluated using an in vitro colonic fermentation system.
32808408	4	88	theme	bacterial	763:771	arg1	groups					773:778	distinct bacterial groups	754:778	distinct bacterial groups of human colonic microbiota	754:806	Changes in populations of distinct bacterial groups of human colonic microbiota induced by digested AB and GB were evaluated using an in vitro colonic fermentation system.
32808408	7	89	theme	non-digested	1167:1178	arg1	AB					1193:1194	AB	1193:1194	AB	1193:1194	Cultivation of probiotics in media with non-digested and digested AB and GB resulted in decreased pH, increased organic acid production and sugar consumption over time.
32808408	6	90	theme	digested	1071:1078	arg1	AB					1080:1081	non-digested and digested AB	1054:1081	non-digested and digested AB	1054:1081	No difference among counts of probiotics in media with glucose, fructooligosaccharides and non-digested and digested AB and GB was found during a 48-h cultivation.
32808408	8	91	theme	in	1589:1590	arg1	fermentation					1606:1617	a 24-h in vitro colonic fermentation	1582:1617	a 24-h in vitro colonic fermentation	1582:1617	Digested AB and GB caused overall beneficial changes in abundance of Bifidobacterium spp., Lactobacillus-Enterococcus, Eubacterium rectall-Clostridium coccoides and Bacteroides-Provotella populations, besides to decrease the pH and increase the short-chain fatty acid production during a 24-h in vitro colonic fermentation.
32808408	2	92	theme	casei	653:657	arg1	subsp					693:697	Lactobacillus acidophilus LA-05, Lactobacillus casei L-26 and Bifidobacterium animalis subsp	606:697	Lactobacillus acidophilus LA-05, Lactobacillus casei L-26 and Bifidobacterium animalis subsp	606:697	METHODS AND RESULTS The ability of non-digested and digested AB or GB to stimulate the growth ad metabolism of Lactobacillus acidophilus LA-05, Lactobacillus casei L-26 and Bifidobacterium animalis subsp.
32808408	4	93	theme	microbiota	797:806	arg1	microbiota					797:806	human colonic microbiota	783:806	human colonic microbiota	783:806	Changes in populations of distinct bacterial groups of human colonic microbiota induced by digested AB and GB were evaluated using an in vitro colonic fermentation system.
32808408	4	93	theme	microbiota	797:806	arg1	groups					773:778	distinct bacterial groups	754:778	distinct bacterial groups of human colonic microbiota	754:806	Changes in populations of distinct bacterial groups of human colonic microbiota induced by digested AB and GB were evaluated using an in vitro colonic fermentation system.
32808408	0	94	dep	Psidium	41:47	arg1	L.					57:58	Psidium guayaba L.	41:58	Psidium guayaba L.	41:58	Acerola (Malpighia glabra L.) and guava (Psidium guayaba L.) industrial processing by-products stimulate probiotic Lactobacillus and Bifidobacterium growth and induce beneficial changes in colonic microbiota.
32808408	6	95	theme	non-digested	1054:1065	arg1	AB					1080:1081	non-digested and digested AB	1054:1081	non-digested and digested AB	1054:1081	No difference among counts of probiotics in media with glucose, fructooligosaccharides and non-digested and digested AB and GB was found during a 48-h cultivation.
32808408	0	96	from	changes	178:184	arg1	microbiota					197:206	colonic microbiota	189:206	colonic microbiota	189:206	Acerola (Malpighia glabra L.) and guava (Psidium guayaba L.) industrial processing by-products stimulate probiotic Lactobacillus and Bifidobacterium growth and induce beneficial changes in colonic microbiota.
32808408	7	97	theme	probiotics	1142:1151	arg1	Cultivation					1127:1137	Cultivation	1127:1137	Cultivation of probiotics in media with non-digested and digested AB and GB	1127:1201	Cultivation of probiotics in media with non-digested and digested AB and GB resulted in decreased pH, increased organic acid production and sugar consumption over time.
32808408	0	98	theme	guava	34:38	arg1	Psidium					41:47	Psidium	41:47	Psidium	41:47	Acerola (Malpighia glabra L.) and guava (Psidium guayaba L.) industrial processing by-products stimulate probiotic Lactobacillus and Bifidobacterium growth and induce beneficial changes in colonic microbiota.
32808408	0	98	theme	guava	34:38	arg1	by-products					83:93	Acerola (Malpighia glabra L.) and guava (Psidium guayaba L.) industrial processing by-products	0:93	by-products	83:93	Acerola (Malpighia glabra L.) and guava (Psidium guayaba L.) industrial processing by-products stimulate probiotic Lactobacillus and Bifidobacterium growth and induce beneficial changes in colonic microbiota.
32808408	7	99	theme	sugar	1267:1271	arg1	consumption					1273:1283	sugar consumption	1267:1283	sugar consumption	1267:1283	Cultivation of probiotics in media with non-digested and digested AB and GB resulted in decreased pH, increased organic acid production and sugar consumption over time.
32808408	11	100	theme	fruit	2120:2124	arg1	by-products					2137:2147	fruit processing by-products	2120:2147	fruit processing by-products	2120:2147	These results characterize AB and GB as potential prebiotic ingredients and fruit processing by-products as sources of added-value compounds.
32808408	11	100	theme	fruit	2120:2124	arg1	GB					2078:2079	GB	2078:2079	GB	2078:2079	These results characterize AB and GB as potential prebiotic ingredients and fruit processing by-products as sources of added-value compounds.
32808408	11	100	theme	fruit	2120:2124	arg1	AB					2071:2072	AB	2071:2072	AB	2071:2072	These results characterize AB and GB as potential prebiotic ingredients and fruit processing by-products as sources of added-value compounds.
32808408	4	101	theme	digested	819:826	arg1	AB					828:829	digested AB	819:829	digested AB	819:829	Changes in populations of distinct bacterial groups of human colonic microbiota induced by digested AB and GB were evaluated using an in vitro colonic fermentation system.
32808408	1	102	theme	colonic	475:481	arg1	microbiota					483:492	human colonic microbiota	469:492	human colonic microbiota	469:492	AIMS This study evaluated whether by-products from industrial processing of acerola (Malpighia glabra L.; AB) and guava (Psidium guajava L.; GB) fruit may stimulate the growth and metabolism of probiotic Lactobacillus and Bifidobacterium and induce changes in human colonic microbiota.
32808408	9	103	theme	probiotics	1734:1743	arg1	metabolism					1720:1729	metabolism	1720:1729	metabolism	1720:1729	CONCLUSION AB and GB could be novel prebiotic ingredients because they can stimulate the growth and metabolism of probiotics and induce overall beneficial changes in human colonic microbiota.
32808408	9	103	theme	probiotics	1734:1743	arg1	growth					1709:1714	growth	1709:1714	growth	1709:1714	CONCLUSION AB and GB could be novel prebiotic ingredients because they can stimulate the growth and metabolism of probiotics and induce overall beneficial changes in human colonic microbiota.
32808408	9	104	theme	novel	1650:1654	arg1	ingredients					1666:1676	novel prebiotic ingredients	1650:1676	novel prebiotic ingredients	1650:1676	CONCLUSION AB and GB could be novel prebiotic ingredients because they can stimulate the growth and metabolism of probiotics and induce overall beneficial changes in human colonic microbiota.
32808408	6	105	theme	48-h	1109:1112	arg1	cultivation					1114:1124	a 48-h cultivation	1107:1124	a 48-h cultivation	1107:1124	No difference among counts of probiotics in media with glucose, fructooligosaccharides and non-digested and digested AB and GB was found during a 48-h cultivation.
32808408	7	106	theme	organic	1239:1245	arg1	production					1252:1261	organic acid production	1239:1261	organic acid production	1239:1261	Cultivation of probiotics in media with non-digested and digested AB and GB resulted in decreased pH, increased organic acid production and sugar consumption over time.
32808408	10	107	dep	induce	1926:1931	arg1	addition					1914:1921	addition	1914:1921	addition	1914:1921	SIGNIFICANCE AND IMPACT OF THE STUDY AB and GB stimulated the growth and metabolism of probiotics, in addition to induce beneficial alterations in human colonic microbiota composition and increase short-chain fatty acid production.
32808408	10	108	theme	human	1959:1963	arg1	composition					1984:1994	human colonic microbiota composition	1959:1994	human colonic microbiota composition	1959:1994	SIGNIFICANCE AND IMPACT OF THE STUDY AB and GB stimulated the growth and metabolism of probiotics, in addition to induce beneficial alterations in human colonic microbiota composition and increase short-chain fatty acid production.
32808408	4	109	theme	colonic	789:795	arg1	microbiota					797:806	human colonic microbiota	783:806	human colonic microbiota	783:806	Changes in populations of distinct bacterial groups of human colonic microbiota induced by digested AB and GB were evaluated using an in vitro colonic fermentation system.
32188070	7	0	theme	LCNF-coated	1037:1047	arg1	samples					1049:1055	LCNF-coated samples	1037:1055	LCNF-coated samples	1037:1055	The observed oxygen transmission rate (OTR) and water vapor permeability (WVP) values of LCNF-coated samples were nearly similar to those with CNF.
32188070	9	1	with	papers	1257:1262	arg1	coat					1274:1277	LCNF coat	1269:1277	LCNF coat	1269:1277	The attained papers with LCNF coat were formed into bowl-like containers using metal molds and a facile oven drying protocol to evaluate their resistance to oil penetration over a longer period.
32188070	2	2	theme	excellent	339:347	arg1	layers					357:362	excellent barrier layers	339:362	excellent barrier layers	339:362	Cellulose nanofibrils (CNF) have been shown by a number of groups to form excellent barrier layers to oxygen and grease.
32188070	9	3	theme	bowl-like	1296:1304	arg1	containers					1306:1315	bowl-like containers	1296:1315	bowl-like containers using metal molds and a facile oven drying protocol to evaluate their resistance to oil penetration over a longer period	1296:1436	The attained papers with LCNF coat were formed into bowl-like containers using metal molds and a facile oven drying protocol to evaluate their resistance to oil penetration over a longer period.
32188070	6	4	theme	water	826:830	arg1	angle					840:844	water contact angle	826:844	water contact angle	826:844	The analysis on the surface free energy and water contact angle pointed to the positive role of lignin distribution in inducing a certain degree of water repellency.
32188070	8	5	theme	oil	1141:1143	arg1	performance					1151:1161	the oil proof performance	1137:1161	the oil proof performance	1137:1161	However, the presence of lignin improved the oil proof performance; these layered designs exhibited an excellent resistance to grease (kit No. 12).
32188070	1	6	theme	poly-	183:187	arg1	compounds					207:215	poly- or perfluorinated compounds	183:215	poly- or perfluorinated compounds	183:215	Environmental and health concerns are driving the need for new materials in food packaging to replace poly- or perfluorinated compounds, aluminum layers, and petroleum-based polymers.
32188070	10	7	theme	LCNF	1479:1482	arg1	layer					1484:1488	LCNF layer	1479:1488	LCNF layer	1479:1488	The results confirmed the capability of LCNF layer in holding commercially available cooking oils with no evidence of leakage for over five months.
32188070	7	8	theme	permeability	1008:1019	arg1	values					1027:1032	The observed oxygen transmission rate (OTR) and water vapor permeability (WVP) values	948:1032	The observed oxygen transmission rate (OTR) and water vapor permeability (WVP) values of LCNF-coated samples	948:1055	The observed oxygen transmission rate (OTR) and water vapor permeability (WVP) values of LCNF-coated samples were nearly similar to those with CNF.
32188070	7	8	theme	permeability	1008:1019	arg1	similar					1069:1075	similar	1069:1075	similar	1069:1075	The observed oxygen transmission rate (OTR) and water vapor permeability (WVP) values of LCNF-coated samples were nearly similar to those with CNF.
32188070	11	9	located	observed	1660:1667	arg2	improvement					1596:1606	an improvement	1593:1606	an improvement in the tensile strength	1593:1630	Also, an improvement in the tensile strength and elongation at break was observed in the studied papers.
32188070	11	9	located	observed	1660:1667	arg1	papers					1684:1689	the studied papers	1672:1689	the studied papers	1672:1689	Also, an improvement in the tensile strength and elongation at break was observed in the studied papers.
32188070	11	9	located	observed	1660:1667	arg2	elongation					1636:1645	elongation	1636:1645	elongation at break	1636:1654	Also, an improvement in the tensile strength and elongation at break was observed in the studied papers.
32188070	1	10	theme	perfluorinated	192:205	arg1	compounds					207:215	poly- or perfluorinated compounds	183:215	poly- or perfluorinated compounds	183:215	Environmental and health concerns are driving the need for new materials in food packaging to replace poly- or perfluorinated compounds, aluminum layers, and petroleum-based polymers.
32188070	7	11	theme	water	996:1000	arg1	WVP					1022:1024	WVP	1022:1024	WVP	1022:1024	The observed oxygen transmission rate (OTR) and water vapor permeability (WVP) values of LCNF-coated samples were nearly similar to those with CNF.
32188070	7	11	theme	water	996:1000	arg1	permeability					1008:1019	water vapor permeability	996:1019	water vapor permeability (WVP)	996:1025	The observed oxygen transmission rate (OTR) and water vapor permeability (WVP) values of LCNF-coated samples were nearly similar to those with CNF.
32188070	12	12	contain	possesses	1733:1741	arg2	architecture					1750:1761	viable architecture	1743:1761	viable architecture	1743:1761	Overall, the proposed packaging material possesses viable architecture and can be considered as a fully wood-based alternative for the current fluorocarbon systems.
32188070	12	12	contain	possesses	1733:1741	arg1	alternative					1807:1817	a fully wood-based alternative	1788:1817	a fully wood-based alternative for the current fluorocarbon systems	1788:1854	Overall, the proposed packaging material possesses viable architecture and can be considered as a fully wood-based alternative for the current fluorocarbon systems.
32188070	12	12	contain	possesses	1733:1741	arg1	material					1724:1731	the proposed packaging material	1701:1731	the proposed packaging material	1701:1731	Overall, the proposed packaging material possesses viable architecture and can be considered as a fully wood-based alternative for the current fluorocarbon systems.
32188070	9	13	theme	oven	1348:1351	arg1	protocol					1360:1367	a facile oven drying protocol	1339:1367	a facile oven drying protocol to evaluate their resistance to oil penetration over a longer period	1339:1436	The attained papers with LCNF coat were formed into bowl-like containers using metal molds and a facile oven drying protocol to evaluate their resistance to oil penetration over a longer period.
32188070	6	14	theme	distribution	885:896	arg1	role					870:873	the positive role	857:873	the positive role of lignin distribution in inducing a certain degree of water repellency	857:945	The analysis on the surface free energy and water contact angle pointed to the positive role of lignin distribution in inducing a certain degree of water repellency.
32188070	3	15	theme	lignin-containing	412:428	arg1	LCNF					453:456	LCNF	453:456	LCNF	453:456	However, the influence of lignin-containing cellulose nanofibrils (LCNF) on film barrier properties has not been well reported.
32188070	3	15	theme	lignin-containing	412:428	arg1	nanofibrils					440:450	lignin-containing cellulose nanofibrils	412:450	lignin-containing cellulose nanofibrils (LCNF)	412:457	However, the influence of lignin-containing cellulose nanofibrils (LCNF) on film barrier properties has not been well reported.
32188070	12	16	theme	current	1827:1833	arg1	systems					1848:1854	the current fluorocarbon systems	1823:1854	the current fluorocarbon systems	1823:1854	Overall, the proposed packaging material possesses viable architecture and can be considered as a fully wood-based alternative for the current fluorocarbon systems.
32188070	11	17	theme	studied	1676:1682	arg1	papers					1684:1689	the studied papers	1672:1689	the studied papers	1672:1689	Also, an improvement in the tensile strength and elongation at break was observed in the studied papers.
32188070	6	18	theme	positive	861:868	arg1	role					870:873	the positive role	857:873	the positive role of lignin distribution in inducing a certain degree of water repellency	857:945	The analysis on the surface free energy and water contact angle pointed to the positive role of lignin distribution in inducing a certain degree of water repellency.
32188070	4	19	theme	wet	674:676	arg1	end					678:680	the wet end	670:680	the wet end of a paper machine	670:699	Herein, thin films (16 g/m2) from LCNF and CNF were formed on paper substrates through a filtration technique that should mimic the addition of material at the wet end of a paper machine.
32188070	11	20	theme	tensile	1615:1621	arg1	strength					1623:1630	the tensile strength	1611:1630	the tensile strength	1611:1630	Also, an improvement in the tensile strength and elongation at break was observed in the studied papers.
32188070	7	21	theme	transmission	968:979	arg1	rate					981:984	oxygen transmission rate	961:984	oxygen transmission rate (OTR)	961:990	The observed oxygen transmission rate (OTR) and water vapor permeability (WVP) values of LCNF-coated samples were nearly similar to those with CNF.
32188070	7	21	theme	transmission	968:979	arg1	OTR					987:989	OTR	987:989	OTR	987:989	The observed oxygen transmission rate (OTR) and water vapor permeability (WVP) values of LCNF-coated samples were nearly similar to those with CNF.
32188070	9	22	theme	oil	1401:1403	arg1	penetration					1405:1415	oil penetration	1401:1415	oil penetration	1401:1415	The attained papers with LCNF coat were formed into bowl-like containers using metal molds and a facile oven drying protocol to evaluate their resistance to oil penetration over a longer period.
32188070	6	23	theme	repellency	936:945	arg1	degree					920:925	a certain degree	910:925	a certain degree of water repellency	910:945	The analysis on the surface free energy and water contact angle pointed to the positive role of lignin distribution in inducing a certain degree of water repellency.
32188070	8	24	theme	kit	1231:1233	arg1	grease					1223:1228	grease	1223:1228	grease (kit No. 12)	1223:1241	However, the presence of lignin improved the oil proof performance; these layered designs exhibited an excellent resistance to grease (kit No. 12).
32188070	8	24	theme	kit	1231:1233	arg1	No.					1235:1237	kit No. 12	1231:1240	kit No. 12	1231:1240	However, the presence of lignin improved the oil proof performance; these layered designs exhibited an excellent resistance to grease (kit No. 12).
32188070	4	25	from	CNF	557:559	arg1	films					527:531	thin films	522:531	thin films (16 g/m2) from LCNF and CNF	522:559	Herein, thin films (16 g/m2) from LCNF and CNF were formed on paper substrates through a filtration technique that should mimic the addition of material at the wet end of a paper machine.
32188070	4	25	from	CNF	557:559	arg1	g/m2					537:540	16 g/m2	534:540	16 g/m2	534:540	Herein, thin films (16 g/m2) from LCNF and CNF were formed on paper substrates through a filtration technique that should mimic the addition of material at the wet end of a paper machine.
32188070	4	26	from	LCNF	548:551	arg1	films					527:531	thin films	522:531	thin films (16 g/m2) from LCNF and CNF	522:559	Herein, thin films (16 g/m2) from LCNF and CNF were formed on paper substrates through a filtration technique that should mimic the addition of material at the wet end of a paper machine.
32188070	4	26	from	LCNF	548:551	arg1	g/m2					537:540	16 g/m2	534:540	16 g/m2	534:540	Herein, thin films (16 g/m2) from LCNF and CNF were formed on paper substrates through a filtration technique that should mimic the addition of material at the wet end of a paper machine.
32188070	9	27	theme	longer	1424:1429	arg1	period					1431:1436	a longer period	1422:1436	a longer period	1422:1436	The attained papers with LCNF coat were formed into bowl-like containers using metal molds and a facile oven drying protocol to evaluate their resistance to oil penetration over a longer period.
32188070	6	28	theme	certain	912:918	arg1	degree					920:925	a certain degree	910:925	a certain degree of water repellency	910:945	The analysis on the surface free energy and water contact angle pointed to the positive role of lignin distribution in inducing a certain degree of water repellency.
32188070	3	29	theme	cellulose	430:438	arg1	LCNF					453:456	LCNF	453:456	LCNF	453:456	However, the influence of lignin-containing cellulose nanofibrils (LCNF) on film barrier properties has not been well reported.
32188070	3	29	theme	cellulose	430:438	arg1	nanofibrils					440:450	lignin-containing cellulose nanofibrils	412:450	lignin-containing cellulose nanofibrils (LCNF)	412:457	However, the influence of lignin-containing cellulose nanofibrils (LCNF) on film barrier properties has not been well reported.
32188070	1	30	dep	Environmental	81:93	arg1	concerns					106:113	concerns	106:113	concerns	106:113	Environmental and health concerns are driving the need for new materials in food packaging to replace poly- or perfluorinated compounds, aluminum layers, and petroleum-based polymers.
32188070	0	31	theme	Paper-Based	0:10	arg1	Packaging					24:32	Paper-Based Oil Barrier Packaging	0:32	Paper-Based Oil Barrier Packaging	0:32	Paper-Based Oil Barrier Packaging using Lignin-Containing Cellulose Nanofibrils.
32188070	4	32	theme	paper	687:691	arg1	machine					693:699	a paper machine	685:699	a paper machine	685:699	Herein, thin films (16 g/m2) from LCNF and CNF were formed on paper substrates through a filtration technique that should mimic the addition of material at the wet end of a paper machine.
32188070	0	33	theme	Barrier	16:22	arg1	Packaging					24:32	Paper-Based Oil Barrier Packaging	0:32	Paper-Based Oil Barrier Packaging	0:32	Paper-Based Oil Barrier Packaging using Lignin-Containing Cellulose Nanofibrils.
32188070	6	34	theme	free	810:813	arg1	energy					815:820	the surface free energy	798:820	the surface free energy	798:820	The analysis on the surface free energy and water contact angle pointed to the positive role of lignin distribution in inducing a certain degree of water repellency.
32188070	12	35	theme	viable	1743:1748	arg1	architecture					1750:1761	viable architecture	1743:1761	viable architecture	1743:1761	Overall, the proposed packaging material possesses viable architecture and can be considered as a fully wood-based alternative for the current fluorocarbon systems.
32188070	0	36	theme	Cellulose	58:66	arg1	Nanofibrils					68:78	Lignin-Containing Cellulose Nanofibrils	40:78	Lignin-Containing Cellulose Nanofibrils	40:78	Paper-Based Oil Barrier Packaging using Lignin-Containing Cellulose Nanofibrils.
32188070	2	37	theme	groups	324:329	arg1	number					314:319	a number	312:319	a number of groups	312:329	Cellulose nanofibrils (CNF) have been shown by a number of groups to form excellent barrier layers to oxygen and grease.
32188070	9	38	theme	LCNF	1269:1272	arg1	coat					1274:1277	LCNF coat	1269:1277	LCNF coat	1269:1277	The attained papers with LCNF coat were formed into bowl-like containers using metal molds and a facile oven drying protocol to evaluate their resistance to oil penetration over a longer period.
32188070	12	39	theme	proposed	1705:1712	arg1	alternative					1807:1817	a fully wood-based alternative	1788:1817	a fully wood-based alternative for the current fluorocarbon systems	1788:1854	Overall, the proposed packaging material possesses viable architecture and can be considered as a fully wood-based alternative for the current fluorocarbon systems.
32188070	12	39	theme	proposed	1705:1712	arg1	material					1724:1731	the proposed packaging material	1701:1731	the proposed packaging material	1701:1731	Overall, the proposed packaging material possesses viable architecture and can be considered as a fully wood-based alternative for the current fluorocarbon systems.
32188070	1	40	theme	food	157:160	arg1	packaging					162:170	food packaging	157:170	food packaging	157:170	Environmental and health concerns are driving the need for new materials in food packaging to replace poly- or perfluorinated compounds, aluminum layers, and petroleum-based polymers.
32188070	1	41	theme	petroleum-based	239:253	arg1	polymers					255:262	petroleum-based polymers	239:262	petroleum-based polymers	239:262	Environmental and health concerns are driving the need for new materials in food packaging to replace poly- or perfluorinated compounds, aluminum layers, and petroleum-based polymers.
32188070	5	42	theme	mechanical	723:732	arg1	attributes					734:743	mechanical attributes	723:743	mechanical attributes	723:743	Surface, barrier and mechanical attributes of these samples were characterized.
32188070	2	43	theme	barrier	349:355	arg1	layers					357:362	excellent barrier layers	339:362	excellent barrier layers	339:362	Cellulose nanofibrils (CNF) have been shown by a number of groups to form excellent barrier layers to oxygen and grease.
32188070	6	44	theme	contact	832:838	arg1	angle					840:844	water contact angle	826:844	water contact angle	826:844	The analysis on the surface free energy and water contact angle pointed to the positive role of lignin distribution in inducing a certain degree of water repellency.
32188070	9	45	theme	metal	1323:1327	arg1	molds					1329:1333	metal molds	1323:1333	metal molds	1323:1333	The attained papers with LCNF coat were formed into bowl-like containers using metal molds and a facile oven drying protocol to evaluate their resistance to oil penetration over a longer period.
32188070	4	46	theme	filtration	603:612	arg1	technique					614:622	a filtration technique	601:622	a filtration technique that should mimic the addition of material at the wet end of a paper machine	601:699	Herein, thin films (16 g/m2) from LCNF and CNF were formed on paper substrates through a filtration technique that should mimic the addition of material at the wet end of a paper machine.
32188070	10	47	theme	layer	1484:1488	arg1	capability					1465:1474	the capability	1461:1474	the capability of LCNF layer	1461:1488	The results confirmed the capability of LCNF layer in holding commercially available cooking oils with no evidence of leakage for over five months.
32188070	7	48	theme	vapor	1002:1006	arg1	WVP					1022:1024	WVP	1022:1024	WVP	1022:1024	The observed oxygen transmission rate (OTR) and water vapor permeability (WVP) values of LCNF-coated samples were nearly similar to those with CNF.
32188070	7	48	theme	vapor	1002:1006	arg1	permeability					1008:1019	water vapor permeability	996:1019	water vapor permeability (WVP)	996:1025	The observed oxygen transmission rate (OTR) and water vapor permeability (WVP) values of LCNF-coated samples were nearly similar to those with CNF.
32188070	12	49	theme	fluorocarbon	1835:1846	arg1	systems					1848:1854	the current fluorocarbon systems	1823:1854	the current fluorocarbon systems	1823:1854	Overall, the proposed packaging material possesses viable architecture and can be considered as a fully wood-based alternative for the current fluorocarbon systems.
32188070	8	50	theme	proof	1145:1149	arg1	performance					1151:1161	the oil proof performance	1137:1161	the oil proof performance	1137:1161	However, the presence of lignin improved the oil proof performance; these layered designs exhibited an excellent resistance to grease (kit No. 12).
32188070	9	51	theme	facile	1341:1346	arg1	protocol					1360:1367	a facile oven drying protocol	1339:1367	a facile oven drying protocol to evaluate their resistance to oil penetration over a longer period	1339:1436	The attained papers with LCNF coat were formed into bowl-like containers using metal molds and a facile oven drying protocol to evaluate their resistance to oil penetration over a longer period.
32188070	11	52	from	elongation	1636:1645	arg1	strength					1623:1630	the tensile strength	1611:1630	the tensile strength	1611:1630	Also, an improvement in the tensile strength and elongation at break was observed in the studied papers.
32188070	4	53	theme	paper	576:580	arg1	substrates					582:591	paper substrates	576:591	paper substrates	576:591	Herein, thin films (16 g/m2) from LCNF and CNF were formed on paper substrates through a filtration technique that should mimic the addition of material at the wet end of a paper machine.
32188070	10	54	theme	available	1514:1522	arg1	oils					1532:1535	commercially available cooking oils	1501:1535	commercially available cooking oils	1501:1535	The results confirmed the capability of LCNF layer in holding commercially available cooking oils with no evidence of leakage for over five months.
32188070	1	55	theme	aluminum	218:225	arg1	layers					227:232	aluminum layers	218:232	aluminum layers	218:232	Environmental and health concerns are driving the need for new materials in food packaging to replace poly- or perfluorinated compounds, aluminum layers, and petroleum-based polymers.
32188070	6	56	theme	lignin	878:883	arg1	distribution					885:896	lignin distribution	878:896	lignin distribution	878:896	The analysis on the surface free energy and water contact angle pointed to the positive role of lignin distribution in inducing a certain degree of water repellency.
32188070	11	57	from	break	1650:1654	arg1	elongation					1636:1645	elongation	1636:1645	elongation at break	1636:1654	Also, an improvement in the tensile strength and elongation at break was observed in the studied papers.
32188070	11	57	from	break	1650:1654	arg1	improvement					1596:1606	an improvement	1593:1606	an improvement in the tensile strength	1593:1630	Also, an improvement in the tensile strength and elongation at break was observed in the studied papers.
32188070	8	58	theme	layered	1170:1176	arg1	designs					1178:1184	these layered designs	1164:1184	these layered designs	1164:1184	However, the presence of lignin improved the oil proof performance; these layered designs exhibited an excellent resistance to grease (kit No. 12).
32188070	6	59	from	analysis	786:793	arg1	energy					815:820	the surface free energy	798:820	the surface free energy	798:820	The analysis on the surface free energy and water contact angle pointed to the positive role of lignin distribution in inducing a certain degree of water repellency.
32188070	6	59	from	analysis	786:793	arg1	angle					840:844	water contact angle	826:844	water contact angle	826:844	The analysis on the surface free energy and water contact angle pointed to the positive role of lignin distribution in inducing a certain degree of water repellency.
32188070	7	60	theme	rate	981:984	arg1	values					1027:1032	The observed oxygen transmission rate (OTR) and water vapor permeability (WVP) values	948:1032	The observed oxygen transmission rate (OTR) and water vapor permeability (WVP) values of LCNF-coated samples	948:1055	The observed oxygen transmission rate (OTR) and water vapor permeability (WVP) values of LCNF-coated samples were nearly similar to those with CNF.
32188070	7	60	theme	rate	981:984	arg1	similar					1069:1075	similar	1069:1075	similar	1069:1075	The observed oxygen transmission rate (OTR) and water vapor permeability (WVP) values of LCNF-coated samples were nearly similar to those with CNF.
32188070	7	61	theme	observed	952:959	arg1	values					1027:1032	The observed oxygen transmission rate (OTR) and water vapor permeability (WVP) values	948:1032	The observed oxygen transmission rate (OTR) and water vapor permeability (WVP) values of LCNF-coated samples	948:1055	The observed oxygen transmission rate (OTR) and water vapor permeability (WVP) values of LCNF-coated samples were nearly similar to those with CNF.
32188070	7	61	theme	observed	952:959	arg1	similar					1069:1075	similar	1069:1075	similar	1069:1075	The observed oxygen transmission rate (OTR) and water vapor permeability (WVP) values of LCNF-coated samples were nearly similar to those with CNF.
32188070	8	62	theme	lignin	1121:1126	arg1	presence					1109:1116	the presence	1105:1116	the presence of lignin	1105:1126	However, the presence of lignin improved the oil proof performance; these layered designs exhibited an excellent resistance to grease (kit No. 12).
32188070	4	63	theme	material	658:665	arg1	addition					646:653	the addition	642:653	the addition of material at the wet end of a paper machine	642:699	Herein, thin films (16 g/m2) from LCNF and CNF were formed on paper substrates through a filtration technique that should mimic the addition of material at the wet end of a paper machine.
32188070	7	64	theme	oxygen	961:966	arg1	rate					981:984	oxygen transmission rate	961:984	oxygen transmission rate (OTR)	961:990	The observed oxygen transmission rate (OTR) and water vapor permeability (WVP) values of LCNF-coated samples were nearly similar to those with CNF.
32188070	7	64	theme	oxygen	961:966	arg1	OTR					987:989	OTR	987:989	OTR	987:989	The observed oxygen transmission rate (OTR) and water vapor permeability (WVP) values of LCNF-coated samples were nearly similar to those with CNF.
32188070	1	65	theme	new	140:142	arg1	materials					144:152	new materials	140:152	new materials in food packaging	140:170	Environmental and health concerns are driving the need for new materials in food packaging to replace poly- or perfluorinated compounds, aluminum layers, and petroleum-based polymers.
32188070	8	66	theme	excellent	1199:1207	arg1	resistance					1209:1218	an excellent resistance	1196:1218	an excellent resistance to grease (kit No. 12)	1196:1241	However, the presence of lignin improved the oil proof performance; these layered designs exhibited an excellent resistance to grease (kit No. 12).
32188070	3	67	theme	film	462:465	arg1	properties					475:484	film barrier properties	462:484	film barrier properties	462:484	However, the influence of lignin-containing cellulose nanofibrils (LCNF) on film barrier properties has not been well reported.
32188070	2	68	theme	Cellulose	265:273	arg1	CNF					288:290	CNF	288:290	CNF	288:290	Cellulose nanofibrils (CNF) have been shown by a number of groups to form excellent barrier layers to oxygen and grease.
32188070	2	68	theme	Cellulose	265:273	arg1	nanofibrils					275:285	Cellulose nanofibrils	265:285	Cellulose nanofibrils (CNF)	265:291	Cellulose nanofibrils (CNF) have been shown by a number of groups to form excellent barrier layers to oxygen and grease.
32188070	6	69	theme	water	930:934	arg1	repellency					936:945	water repellency	930:945	water repellency	930:945	The analysis on the surface free energy and water contact angle pointed to the positive role of lignin distribution in inducing a certain degree of water repellency.
32188070	11	70	from	improvement	1596:1606	arg1	strength					1623:1630	the tensile strength	1611:1630	the tensile strength	1611:1630	Also, an improvement in the tensile strength and elongation at break was observed in the studied papers.
32188070	3	71	from	influence	399:407	arg1	properties					475:484	film barrier properties	462:484	film barrier properties	462:484	However, the influence of lignin-containing cellulose nanofibrils (LCNF) on film barrier properties has not been well reported.
32188070	12	72	theme	wood-based	1796:1805	arg1	alternative					1807:1817	a fully wood-based alternative	1788:1817	a fully wood-based alternative for the current fluorocarbon systems	1788:1854	Overall, the proposed packaging material possesses viable architecture and can be considered as a fully wood-based alternative for the current fluorocarbon systems.
32188070	12	72	theme	wood-based	1796:1805	arg1	material					1724:1731	the proposed packaging material	1701:1731	the proposed packaging material	1701:1731	Overall, the proposed packaging material possesses viable architecture and can be considered as a fully wood-based alternative for the current fluorocarbon systems.
32188070	4	73	theme	machine	693:699	arg1	end					678:680	the wet end	670:680	the wet end of a paper machine	670:699	Herein, thin films (16 g/m2) from LCNF and CNF were formed on paper substrates through a filtration technique that should mimic the addition of material at the wet end of a paper machine.
32188070	3	74	theme	nanofibrils	440:450	arg1	influence					399:407	the influence	395:407	the influence of lignin-containing cellulose nanofibrils (LCNF) on film barrier properties	395:484	However, the influence of lignin-containing cellulose nanofibrils (LCNF) on film barrier properties has not been well reported.
32188070	0	75	theme	Oil	12:14	arg1	Packaging					24:32	Paper-Based Oil Barrier Packaging	0:32	Paper-Based Oil Barrier Packaging	0:32	Paper-Based Oil Barrier Packaging using Lignin-Containing Cellulose Nanofibrils.
32188070	1	76	from	materials	144:152	arg1	packaging					162:170	food packaging	157:170	food packaging	157:170	Environmental and health concerns are driving the need for new materials in food packaging to replace poly- or perfluorinated compounds, aluminum layers, and petroleum-based polymers.
32188070	10	77	theme	cooking	1524:1530	arg1	oils					1532:1535	commercially available cooking oils	1501:1535	commercially available cooking oils	1501:1535	The results confirmed the capability of LCNF layer in holding commercially available cooking oils with no evidence of leakage for over five months.
32188070	5	78	theme	samples	754:760	arg1	barrier					711:717	barrier	711:717	barrier	711:717	Surface, barrier and mechanical attributes of these samples were characterized.
32188070	5	78	theme	samples	754:760	arg1	attributes					734:743	mechanical attributes	723:743	mechanical attributes	723:743	Surface, barrier and mechanical attributes of these samples were characterized.
32188070	5	78	theme	samples	754:760	arg1	Surface					702:708	Surface	702:708	Surface	702:708	Surface, barrier and mechanical attributes of these samples were characterized.
32188070	4	79	from	end	678:680	arg1	addition					646:653	the addition	642:653	the addition of material at the wet end of a paper machine	642:699	Herein, thin films (16 g/m2) from LCNF and CNF were formed on paper substrates through a filtration technique that should mimic the addition of material at the wet end of a paper machine.
32188070	10	80	theme	leakage	1557:1563	arg1	evidence					1545:1552	no evidence	1542:1552	no evidence of leakage for over five months	1542:1584	The results confirmed the capability of LCNF layer in holding commercially available cooking oils with no evidence of leakage for over five months.
32188070	0	81	theme	Lignin-Containing	40:56	arg1	Nanofibrils					68:78	Lignin-Containing Cellulose Nanofibrils	40:78	Lignin-Containing Cellulose Nanofibrils	40:78	Paper-Based Oil Barrier Packaging using Lignin-Containing Cellulose Nanofibrils.
32188070	6	82	theme	surface	802:808	arg1	energy					815:820	the surface free energy	798:820	the surface free energy	798:820	The analysis on the surface free energy and water contact angle pointed to the positive role of lignin distribution in inducing a certain degree of water repellency.
32188070	7	83	theme	samples	1049:1055	arg1	values					1027:1032	The observed oxygen transmission rate (OTR) and water vapor permeability (WVP) values	948:1032	The observed oxygen transmission rate (OTR) and water vapor permeability (WVP) values of LCNF-coated samples	948:1055	The observed oxygen transmission rate (OTR) and water vapor permeability (WVP) values of LCNF-coated samples were nearly similar to those with CNF.
32188070	7	83	theme	samples	1049:1055	arg1	similar					1069:1075	similar	1069:1075	similar	1069:1075	The observed oxygen transmission rate (OTR) and water vapor permeability (WVP) values of LCNF-coated samples were nearly similar to those with CNF.
32188070	9	84	theme	attained	1248:1255	arg1	papers					1257:1262	The attained papers	1244:1262	The attained papers with LCNF coat	1244:1277	The attained papers with LCNF coat were formed into bowl-like containers using metal molds and a facile oven drying protocol to evaluate their resistance to oil penetration over a longer period.
32188070	9	85	theme	drying	1353:1358	arg1	protocol					1360:1367	a facile oven drying protocol	1339:1367	a facile oven drying protocol to evaluate their resistance to oil penetration over a longer period	1339:1436	The attained papers with LCNF coat were formed into bowl-like containers using metal molds and a facile oven drying protocol to evaluate their resistance to oil penetration over a longer period.
32188070	4	86	theme	thin	522:525	arg1	films					527:531	thin films	522:531	thin films (16 g/m2) from LCNF and CNF	522:559	Herein, thin films (16 g/m2) from LCNF and CNF were formed on paper substrates through a filtration technique that should mimic the addition of material at the wet end of a paper machine.
32188070	4	86	theme	thin	522:525	arg1	g/m2					537:540	16 g/m2	534:540	16 g/m2	534:540	Herein, thin films (16 g/m2) from LCNF and CNF were formed on paper substrates through a filtration technique that should mimic the addition of material at the wet end of a paper machine.
32188070	12	87	theme	packaging	1714:1722	arg1	alternative					1807:1817	a fully wood-based alternative	1788:1817	a fully wood-based alternative for the current fluorocarbon systems	1788:1854	Overall, the proposed packaging material possesses viable architecture and can be considered as a fully wood-based alternative for the current fluorocarbon systems.
32188070	12	87	theme	packaging	1714:1722	arg1	material					1724:1731	the proposed packaging material	1701:1731	the proposed packaging material	1701:1731	Overall, the proposed packaging material possesses viable architecture and can be considered as a fully wood-based alternative for the current fluorocarbon systems.
32188070	3	88	theme	barrier	467:473	arg1	properties					475:484	film barrier properties	462:484	film barrier properties	462:484	However, the influence of lignin-containing cellulose nanofibrils (LCNF) on film barrier properties has not been well reported.
32823689	2	0	theme	nutrient	422:429	arg1	assurance					438:446	nutrient intake assurance	422:446	other regions as well as nutrient intake assurance	397:446	The present study aimed to determine the FODMAP consumption of Spanish children, adolescents and adults, analyzing the real FODMAP risk of foods, and to set an open methodology for the measurement of this intake in other regions as well as nutrient intake assurance.
32823689	2	1	theme	foods	321:325	arg1	risk					313:316	the real FODMAP risk	297:316	the real FODMAP risk of foods	297:325	The present study aimed to determine the FODMAP consumption of Spanish children, adolescents and adults, analyzing the real FODMAP risk of foods, and to set an open methodology for the measurement of this intake in other regions as well as nutrient intake assurance.
32823689	6	2	theme	±	924:924	arg1	g/day					931:935	21.4 ± 56.7 g/day	919:935	21.4 ± 56.7 g/day	919:935	Both intakes were higher than that of the adult population (21.4 ± 56.7 g/day).
32823689	6	2	theme	±	924:924	arg1	population					907:916	the adult population	897:916	the adult population (21.4 ± 56.7 g/day)	897:936	Both intakes were higher than that of the adult population (21.4 ± 56.7 g/day).
32823689	0	3	from	Intake	7:12	arg1	Population					25:34	Spanish Population	17:34	Spanish Population	17:34	FODMAP Intake in Spanish Population: Open Approach for Risk Assessment.
32823689	10	4	theme	nutrient	1370:1377	arg1	control					1379:1385	nutrient control	1370:1385	nutrient control	1370:1385	An open approach for FODMAP intake quantification and nutrient control was provided.
32823689	3	5	theme	Total	449:453	arg1	analysis					463:470	Total fructan analysis	449:470	Total fructan analysis	449:470	Total fructan analysis was performed analytically in eighty-seven food samples.
32823689	4	6	theme	Daily	529:533	arg1	intake					535:540	Daily intake	529:540	Daily intake of FODMAPs, fiber and micronutrients	529:577	Daily intake of FODMAPs, fiber and micronutrients was calculated by combining the food composition for selected fermentable carbohydrates with the national food consumption stratified by age in an open software.
32823689	10	7	theme	open	1319:1322	arg1	approach					1324:1331	An open approach	1316:1331	An open approach for FODMAP intake quantification and nutrient control	1316:1385	An open approach for FODMAP intake quantification and nutrient control was provided.
32823689	10	8	theme	FODMAP	1337:1342	arg1	quantification					1351:1364	FODMAP intake quantification	1337:1364	FODMAP intake quantification	1337:1364	An open approach for FODMAP intake quantification and nutrient control was provided.
32823689	2	9	theme	adolescents	263:273	arg1	consumption					230:240	the FODMAP consumption	219:240	the FODMAP consumption of Spanish children, adolescents and adults	219:284	The present study aimed to determine the FODMAP consumption of Spanish children, adolescents and adults, analyzing the real FODMAP risk of foods, and to set an open methodology for the measurement of this intake in other regions as well as nutrient intake assurance.
32823689	4	10	theme	FODMAPs	545:551	arg1	intake					535:540	Daily intake	529:540	Daily intake of FODMAPs, fiber and micronutrients	529:577	Daily intake of FODMAPs, fiber and micronutrients was calculated by combining the food composition for selected fermentable carbohydrates with the national food consumption stratified by age in an open software.
32823689	0	11	theme	FODMAP	0:5	arg1	Intake					7:12	FODMAP Intake	0:12	FODMAP Intake in Spanish Population: Open Approach for Risk Assessment.	0:70	FODMAP Intake in Spanish Population: Open Approach for Risk Assessment.
32823689	7	12	theme	total	1006:1010	arg1	fructan					1012:1018	fructose and total fructan	993:1018	fructan	1012:1018	The most important food sources of lactose, excess of fructose and total fructan, considering their content and dietary intake were different between age groups.
32823689	2	13	theme	children	253:260	arg1	consumption					230:240	the FODMAP consumption	219:240	the FODMAP consumption of Spanish children, adolescents and adults	219:284	The present study aimed to determine the FODMAP consumption of Spanish children, adolescents and adults, analyzing the real FODMAP risk of foods, and to set an open methodology for the measurement of this intake in other regions as well as nutrient intake assurance.
32823689	2	14	theme	open	342:345	arg1	methodology					347:357	an open methodology	339:357	an open methodology for the measurement of this intake	339:392	The present study aimed to determine the FODMAP consumption of Spanish children, adolescents and adults, analyzing the real FODMAP risk of foods, and to set an open methodology for the measurement of this intake in other regions as well as nutrient intake assurance.
32823689	0	15	theme	Open	37:40	arg1	Approach					42:49	Open Approach	37:49	FODMAP Intake in Spanish Population: Open Approach for Risk Assessment.	0:70	FODMAP Intake in Spanish Population: Open Approach for Risk Assessment.
32823689	7	16	theme	fructan	1012:1018	arg1	excess					983:988	lactose, excess	974:988	excess	983:988	The most important food sources of lactose, excess of fructose and total fructan, considering their content and dietary intake were different between age groups.
32823689	2	17	theme	Spanish	245:251	arg1	children					253:260	Spanish children	245:260	Spanish children	245:260	The present study aimed to determine the FODMAP consumption of Spanish children, adolescents and adults, analyzing the real FODMAP risk of foods, and to set an open methodology for the measurement of this intake in other regions as well as nutrient intake assurance.
32823689	9	18	theme	FODMAP	1294:1299	arg1	intake					1301:1306	the total FODMAP intake	1284:1306	the total FODMAP intake	1284:1306	We demonstrated the relevance of stratifying the total FODMAP intake by age.
32823689	5	19	theme	total	770:774	arg1	consumption					783:793	adolescent total FODMAP consumption	759:793	adolescent total FODMAP consumption	759:793	Spanish child and adolescent total FODMAP consumption was settled as 33.4 ± 92.4 and 27.3 ± 69.0 g/day, respectively.
32823689	5	19	theme	total	770:774	arg1	g/day					838:842	33.4 ± 92.4 and 27.3 ± 69.0 g/day	810:842	33.4 ± 92.4 and 27.3 ± 69.0 g/day	810:842	Spanish child and adolescent total FODMAP consumption was settled as 33.4 ± 92.4 and 27.3 ± 69.0 g/day, respectively.
32823689	5	19	theme	total	770:774	arg1	child					749:753	Spanish child	741:753	Spanish child	741:753	Spanish child and adolescent total FODMAP consumption was settled as 33.4 ± 92.4 and 27.3 ± 69.0 g/day, respectively.
32823689	7	20	theme	fructose	993:1000	arg1	fructan					1012:1018	fructose and total fructan	993:1018	fructan	1012:1018	The most important food sources of lactose, excess of fructose and total fructan, considering their content and dietary intake were different between age groups.
32823689	4	21	from	age	716:718	arg1	software					731:738	an open software	723:738	an open software	723:738	Daily intake of FODMAPs, fiber and micronutrients was calculated by combining the food composition for selected fermentable carbohydrates with the national food consumption stratified by age in an open software.
32823689	1	22	theme	Fermentable	72:82	arg1	oligo-					84:89	Fermentable oligo-	72:89	Fermentable oligo-	72:89	Fermentable oligo-, di- and monosaccharides and polyols' (FODMAP) were related with intestinal complications.
32823689	7	23	theme	age	1089:1091	arg1	groups					1093:1098	age groups	1089:1098	age groups	1089:1098	The most important food sources of lactose, excess of fructose and total fructan, considering their content and dietary intake were different between age groups.
32823689	2	24	theme	intake	387:392	arg1	measurement					367:377	the measurement	363:377	the measurement of this intake	363:392	The present study aimed to determine the FODMAP consumption of Spanish children, adolescents and adults, analyzing the real FODMAP risk of foods, and to set an open methodology for the measurement of this intake in other regions as well as nutrient intake assurance.
32823689	8	25	theme	foods	1127:1131	arg1	contribution					1105:1116	The contribution	1101:1116	The contribution of these foods to dietary calcium and fiber and the consequent risk of deficiency if they are withdrawn	1101:1220	The contribution of these foods to dietary calcium and fiber and the consequent risk of deficiency if they are withdrawn was highlighted.
32823689	5	26	theme	FODMAP	776:781	arg1	consumption					783:793	adolescent total FODMAP consumption	759:793	adolescent total FODMAP consumption	759:793	Spanish child and adolescent total FODMAP consumption was settled as 33.4 ± 92.4 and 27.3 ± 69.0 g/day, respectively.
32823689	5	26	theme	FODMAP	776:781	arg1	g/day					838:842	33.4 ± 92.4 and 27.3 ± 69.0 g/day	810:842	33.4 ± 92.4 and 27.3 ± 69.0 g/day	810:842	Spanish child and adolescent total FODMAP consumption was settled as 33.4 ± 92.4 and 27.3 ± 69.0 g/day, respectively.
32823689	5	26	theme	FODMAP	776:781	arg1	child					749:753	Spanish child	741:753	Spanish child	741:753	Spanish child and adolescent total FODMAP consumption was settled as 33.4 ± 92.4 and 27.3 ± 69.0 g/day, respectively.
32823689	1	27	theme	intestinal	156:165	arg1	complications					167:179	intestinal complications	156:179	intestinal complications	156:179	Fermentable oligo-, di- and monosaccharides and polyols' (FODMAP) were related with intestinal complications.
32823689	0	28	theme	Spanish	17:23	arg1	Population					25:34	Spanish Population	17:34	Spanish Population	17:34	FODMAP Intake in Spanish Population: Open Approach for Risk Assessment.
32823689	0	29	dep	Intake	7:12	arg1	Approach					42:49	Open Approach	37:49	FODMAP Intake in Spanish Population: Open Approach for Risk Assessment.	0:70	FODMAP Intake in Spanish Population: Open Approach for Risk Assessment.
32823689	7	30	theme	excess	983:988	arg1	sources					963:969	The most important food sources	939:969	The most important food sources	939:969	The most important food sources of lactose, excess of fructose and total fructan, considering their content and dietary intake were different between age groups.
32823689	7	30	theme	excess	983:988	arg1	different					1071:1079	different	1071:1079	different	1071:1079	The most important food sources of lactose, excess of fructose and total fructan, considering their content and dietary intake were different between age groups.
32823689	2	31	theme	adults	279:284	arg1	consumption					230:240	the FODMAP consumption	219:240	the FODMAP consumption of Spanish children, adolescents and adults	219:284	The present study aimed to determine the FODMAP consumption of Spanish children, adolescents and adults, analyzing the real FODMAP risk of foods, and to set an open methodology for the measurement of this intake in other regions as well as nutrient intake assurance.
32823689	8	32	theme	consequent	1170:1179	arg1	risk					1181:1184	the consequent risk	1166:1184	the consequent risk of deficiency	1166:1198	The contribution of these foods to dietary calcium and fiber and the consequent risk of deficiency if they are withdrawn was highlighted.
32823689	8	33	dep	fiber	1156:1160	arg1	withdrawn					1212:1220	withdrawn	1212:1220	are withdrawn	1208:1220	The contribution of these foods to dietary calcium and fiber and the consequent risk of deficiency if they are withdrawn was highlighted.
32823689	6	34	theme	adult	901:905	arg1	g/day					931:935	21.4 ± 56.7 g/day	919:935	21.4 ± 56.7 g/day	919:935	Both intakes were higher than that of the adult population (21.4 ± 56.7 g/day).
32823689	6	34	theme	adult	901:905	arg1	population					907:916	the adult population	897:916	the adult population (21.4 ± 56.7 g/day)	897:936	Both intakes were higher than that of the adult population (21.4 ± 56.7 g/day).
32823689	3	35	theme	food	515:518	arg1	samples					520:526	eighty-seven food samples	502:526	eighty-seven food samples	502:526	Total fructan analysis was performed analytically in eighty-seven food samples.
32823689	4	36	theme	fermentable	641:651	arg1	carbohydrates					653:665	selected fermentable carbohydrates	632:665	selected fermentable carbohydrates	632:665	Daily intake of FODMAPs, fiber and micronutrients was calculated by combining the food composition for selected fermentable carbohydrates with the national food consumption stratified by age in an open software.
32823689	5	37	theme	Spanish	741:747	arg1	consumption					783:793	adolescent total FODMAP consumption	759:793	adolescent total FODMAP consumption	759:793	Spanish child and adolescent total FODMAP consumption was settled as 33.4 ± 92.4 and 27.3 ± 69.0 g/day, respectively.
32823689	5	37	theme	Spanish	741:747	arg1	child					749:753	Spanish child	741:753	Spanish child	741:753	Spanish child and adolescent total FODMAP consumption was settled as 33.4 ± 92.4 and 27.3 ± 69.0 g/day, respectively.
32823689	5	37	theme	Spanish	741:747	arg1	g/day					838:842	33.4 ± 92.4 and 27.3 ± 69.0 g/day	810:842	33.4 ± 92.4 and 27.3 ± 69.0 g/day	810:842	Spanish child and adolescent total FODMAP consumption was settled as 33.4 ± 92.4 and 27.3 ± 69.0 g/day, respectively.
32823689	8	38	theme	deficiency	1189:1198	arg1	fiber					1156:1160	fiber	1156:1160	fiber	1156:1160	The contribution of these foods to dietary calcium and fiber and the consequent risk of deficiency if they are withdrawn was highlighted.
32823689	8	38	theme	deficiency	1189:1198	arg1	calcium					1144:1150	dietary calcium	1136:1150	dietary calcium	1136:1150	The contribution of these foods to dietary calcium and fiber and the consequent risk of deficiency if they are withdrawn was highlighted.
32823689	8	38	theme	deficiency	1189:1198	arg1	risk					1181:1184	the consequent risk	1166:1184	the consequent risk of deficiency	1166:1198	The contribution of these foods to dietary calcium and fiber and the consequent risk of deficiency if they are withdrawn was highlighted.
32823689	7	39	theme	lactose	974:980	arg1	excess					983:988	lactose, excess	974:988	excess	983:988	The most important food sources of lactose, excess of fructose and total fructan, considering their content and dietary intake were different between age groups.
32823689	4	40	theme	food	611:614	arg1	composition					616:626	the food composition	607:626	the food composition for selected fermentable carbohydrates	607:665	Daily intake of FODMAPs, fiber and micronutrients was calculated by combining the food composition for selected fermentable carbohydrates with the national food consumption stratified by age in an open software.
32823689	2	41	from	set	335:337	arg1	regions					403:409	other regions	397:409	other regions as well as nutrient intake assurance	397:446	The present study aimed to determine the FODMAP consumption of Spanish children, adolescents and adults, analyzing the real FODMAP risk of foods, and to set an open methodology for the measurement of this intake in other regions as well as nutrient intake assurance.
32823689	2	41	from	set	335:337	arg1	assurance					438:446	nutrient intake assurance	422:446	other regions as well as nutrient intake assurance	397:446	The present study aimed to determine the FODMAP consumption of Spanish children, adolescents and adults, analyzing the real FODMAP risk of foods, and to set an open methodology for the measurement of this intake in other regions as well as nutrient intake assurance.
32823689	5	42	theme	±	815:815	arg1	consumption					783:793	adolescent total FODMAP consumption	759:793	adolescent total FODMAP consumption	759:793	Spanish child and adolescent total FODMAP consumption was settled as 33.4 ± 92.4 and 27.3 ± 69.0 g/day, respectively.
32823689	5	42	theme	±	815:815	arg1	g/day					838:842	33.4 ± 92.4 and 27.3 ± 69.0 g/day	810:842	33.4 ± 92.4 and 27.3 ± 69.0 g/day	810:842	Spanish child and adolescent total FODMAP consumption was settled as 33.4 ± 92.4 and 27.3 ± 69.0 g/day, respectively.
32823689	5	42	theme	±	815:815	arg1	child					749:753	Spanish child	741:753	Spanish child	741:753	Spanish child and adolescent total FODMAP consumption was settled as 33.4 ± 92.4 and 27.3 ± 69.0 g/day, respectively.
32823689	8	43	theme	dietary	1136:1142	arg1	calcium					1144:1150	dietary calcium	1136:1150	dietary calcium	1136:1150	The contribution of these foods to dietary calcium and fiber and the consequent risk of deficiency if they are withdrawn was highlighted.
32823689	5	44	theme	adolescent	759:768	arg1	consumption					783:793	adolescent total FODMAP consumption	759:793	adolescent total FODMAP consumption	759:793	Spanish child and adolescent total FODMAP consumption was settled as 33.4 ± 92.4 and 27.3 ± 69.0 g/day, respectively.
32823689	5	44	theme	adolescent	759:768	arg1	g/day					838:842	33.4 ± 92.4 and 27.3 ± 69.0 g/day	810:842	33.4 ± 92.4 and 27.3 ± 69.0 g/day	810:842	Spanish child and adolescent total FODMAP consumption was settled as 33.4 ± 92.4 and 27.3 ± 69.0 g/day, respectively.
32823689	5	44	theme	adolescent	759:768	arg1	child					749:753	Spanish child	741:753	Spanish child	741:753	Spanish child and adolescent total FODMAP consumption was settled as 33.4 ± 92.4 and 27.3 ± 69.0 g/day, respectively.
32823689	10	45	theme	intake	1344:1349	arg1	quantification					1351:1364	FODMAP intake quantification	1337:1364	FODMAP intake quantification	1337:1364	An open approach for FODMAP intake quantification and nutrient control was provided.
32823689	4	46	theme	national	676:683	arg1	consumption					690:700	the national food consumption	672:700	the national food consumption stratified by age in an open software	672:738	Daily intake of FODMAPs, fiber and micronutrients was calculated by combining the food composition for selected fermentable carbohydrates with the national food consumption stratified by age in an open software.
32823689	2	47	theme	other	397:401	arg1	regions					403:409	other regions	397:409	other regions as well as nutrient intake assurance	397:446	The present study aimed to determine the FODMAP consumption of Spanish children, adolescents and adults, analyzing the real FODMAP risk of foods, and to set an open methodology for the measurement of this intake in other regions as well as nutrient intake assurance.
32823689	2	48	theme	present	186:192	arg1	study					194:198	The present study	182:198	The present study	182:198	The present study aimed to determine the FODMAP consumption of Spanish children, adolescents and adults, analyzing the real FODMAP risk of foods, and to set an open methodology for the measurement of this intake in other regions as well as nutrient intake assurance.
32823689	2	49	theme	FODMAP	306:311	arg1	risk					313:316	the real FODMAP risk	297:316	the real FODMAP risk of foods	297:325	The present study aimed to determine the FODMAP consumption of Spanish children, adolescents and adults, analyzing the real FODMAP risk of foods, and to set an open methodology for the measurement of this intake in other regions as well as nutrient intake assurance.
32823689	9	50	theme	total	1288:1292	arg1	intake					1301:1306	the total FODMAP intake	1284:1306	the total FODMAP intake	1284:1306	We demonstrated the relevance of stratifying the total FODMAP intake by age.
32823689	7	51	theme	important	948:956	arg1	sources					963:969	The most important food sources	939:969	The most important food sources	939:969	The most important food sources of lactose, excess of fructose and total fructan, considering their content and dietary intake were different between age groups.
32823689	7	51	theme	important	948:956	arg1	different					1071:1079	different	1071:1079	different	1071:1079	The most important food sources of lactose, excess of fructose and total fructan, considering their content and dietary intake were different between age groups.
32823689	7	52	theme	dietary	1051:1057	arg1	intake					1059:1064	dietary intake	1051:1064	dietary intake	1051:1064	The most important food sources of lactose, excess of fructose and total fructan, considering their content and dietary intake were different between age groups.
32823689	2	53	theme	real	301:304	arg1	risk					313:316	the real FODMAP risk	297:316	the real FODMAP risk of foods	297:325	The present study aimed to determine the FODMAP consumption of Spanish children, adolescents and adults, analyzing the real FODMAP risk of foods, and to set an open methodology for the measurement of this intake in other regions as well as nutrient intake assurance.
32823689	3	54	theme	eighty-seven	502:513	arg1	samples					520:526	eighty-seven food samples	502:526	eighty-seven food samples	502:526	Total fructan analysis was performed analytically in eighty-seven food samples.
32823689	4	55	theme	fiber	554:558	arg1	intake					535:540	Daily intake	529:540	Daily intake of FODMAPs, fiber and micronutrients	529:577	Daily intake of FODMAPs, fiber and micronutrients was calculated by combining the food composition for selected fermentable carbohydrates with the national food consumption stratified by age in an open software.
32823689	7	56	theme	food	958:961	arg1	sources					963:969	The most important food sources	939:969	The most important food sources	939:969	The most important food sources of lactose, excess of fructose and total fructan, considering their content and dietary intake were different between age groups.
32823689	7	56	theme	food	958:961	arg1	different					1071:1079	different	1071:1079	different	1071:1079	The most important food sources of lactose, excess of fructose and total fructan, considering their content and dietary intake were different between age groups.
32823689	4	57	theme	open	726:729	arg1	software					731:738	an open software	723:738	an open software	723:738	Daily intake of FODMAPs, fiber and micronutrients was calculated by combining the food composition for selected fermentable carbohydrates with the national food consumption stratified by age in an open software.
32823689	4	58	theme	food	685:688	arg1	consumption					690:700	the national food consumption	672:700	the national food consumption stratified by age in an open software	672:738	Daily intake of FODMAPs, fiber and micronutrients was calculated by combining the food composition for selected fermentable carbohydrates with the national food consumption stratified by age in an open software.
32823689	0	59	theme	Risk	55:58	arg1	Assessment					60:69	Risk Assessment	55:69	Risk Assessment	55:69	FODMAP Intake in Spanish Population: Open Approach for Risk Assessment.
32823689	2	60	theme	FODMAP	223:228	arg1	consumption					230:240	the FODMAP consumption	219:240	the FODMAP consumption of Spanish children, adolescents and adults	219:284	The present study aimed to determine the FODMAP consumption of Spanish children, adolescents and adults, analyzing the real FODMAP risk of foods, and to set an open methodology for the measurement of this intake in other regions as well as nutrient intake assurance.
32823689	3	61	theme	fructan	455:461	arg1	analysis					463:470	Total fructan analysis	449:470	Total fructan analysis	449:470	Total fructan analysis was performed analytically in eighty-seven food samples.
32823689	4	62	theme	micronutrients	564:577	arg1	intake					535:540	Daily intake	529:540	Daily intake of FODMAPs, fiber and micronutrients	529:577	Daily intake of FODMAPs, fiber and micronutrients was calculated by combining the food composition for selected fermentable carbohydrates with the national food consumption stratified by age in an open software.
32823689	2	63	theme	intake	431:436	arg1	assurance					438:446	nutrient intake assurance	422:446	other regions as well as nutrient intake assurance	397:446	The present study aimed to determine the FODMAP consumption of Spanish children, adolescents and adults, analyzing the real FODMAP risk of foods, and to set an open methodology for the measurement of this intake in other regions as well as nutrient intake assurance.
32823689	4	64	theme	selected	632:639	arg1	carbohydrates					653:665	selected fermentable carbohydrates	632:665	selected fermentable carbohydrates	632:665	Daily intake of FODMAPs, fiber and micronutrients was calculated by combining the food composition for selected fermentable carbohydrates with the national food consumption stratified by age in an open software.
33663865	3	0	theme	fecal	656:660	arg1	microbiome					662:671	the fecal microbiome	652:671	the fecal microbiome	652:671	Therefore, the aims of this study were to determine the effects of an intermittent high-grain SARA model on the hindgut microbial community, and to evaluate whether the effects of SARA on the fecal microbiome and fermentation were further affected by an intramammary lipopolysaccharide (LPS) challenge.
33663865	8	1	theme	Shannon	1625:1631	arg1	indices					1643:1649	the Shannon and Chao1 indices	1621:1649	indices	1643:1649	On d 30, SARA-challenged cows had decreased fecal diversity as shown by the Shannon and Chao1 indices and a decrease in the relative abundance of Euryarchaeota and cellulolytic genera, and numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation.
33663865	2	2	theme	hindgut	354:360	arg1	microbiota					362:371	hindgut microbiota	354:371	hindgut microbiota	354:371	Yet, the effect of SARA on hindgut microbiota, and whether this condition is aggravated by exogenous immune stimuli, is less understood.
33663865	2	3	from	effect	336:341	arg1	microbiota					362:371	hindgut microbiota	354:371	hindgut microbiota	354:371	Yet, the effect of SARA on hindgut microbiota, and whether this condition is aggravated by exogenous immune stimuli, is less understood.
33663865	7	4	from	proportion	1491:1500	arg1	feces					1542:1546	the feces	1538:1546	the feces	1538:1546	The SARA-inducing feeding challenge resulted in decreased fecal pH and increased butyrate as a proportion of total short-chain fatty acids in the feces.
33663865	4	5	theme	%	944:944	arg1	concentrate					946:956	40% concentrate	942:956	40% concentrate	942:956	A total of 18 early-lactating Simmental cows were divided into 3 groups (n = 6); 2 were fed a SARA-inducing feeding regimen (60% concentrate), 1 was fed a control (CON) diet (40% concentrate).
33663865	4	5	theme	%	944:944	arg1	diet					936:939	a control (CON) diet	920:939	a control (CON) diet (40% concentrate)	920:957	A total of 18 early-lactating Simmental cows were divided into 3 groups (n = 6); 2 were fed a SARA-inducing feeding regimen (60% concentrate), 1 was fed a control (CON) diet (40% concentrate).
33663865	9	6	theme	short-chain	1907:1917	arg1	acids					1925:1929	short-chain fatty acids	1907:1929	short-chain fatty acids	1907:1929	The LPS challenge did not affect the fecal pH and short-chain fatty acids, but increased the Chao1 richness index in an interaction with the SARA challenge, and affected the relative abundance of Verrucomicrobia (1.13%), Actinobacteria (0.19%), and Spirochaetes (0.002%), suggesting an effect on the microbial ecology of the hindgut during SARA conditions.
33663865	8	7	theme	Chao1	1637:1641	arg1	indices					1643:1649	the Shannon and Chao1 indices	1621:1649	indices	1643:1649	On d 30, SARA-challenged cows had decreased fecal diversity as shown by the Shannon and Chao1 indices and a decrease in the relative abundance of Euryarchaeota and cellulolytic genera, and numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation.
33663865	9	8	theme	Spirochaetes	2106:2117	arg1	abundance					2040:2048	the relative abundance	2027:2048	the relative abundance of Verrucomicrobia (1.13%), Actinobacteria (0.19%), and Spirochaetes (0.002%)	2027:2126	The LPS challenge did not affect the fecal pH and short-chain fatty acids, but increased the Chao1 richness index in an interaction with the SARA challenge, and affected the relative abundance of Verrucomicrobia (1.13%), Actinobacteria (0.19%), and Spirochaetes (0.002%), suggesting an effect on the microbial ecology of the hindgut during SARA conditions.
33663865	1	9	theme	ruminal	282:288	arg1	dysbiosis					290:298	ruminal dysbiosis	282:298	ruminal dysbiosis	282:298	Feeding grain-rich diets often results in subacute ruminal acidosis (SARA), a condition associated with ruminal dysbiosis and systemic inflammation.
33663865	5	10	from	µg	1077:1078	arg1	Escherichia					1092:1102	Escherichia	1092:1102	Escherichia	1092:1102	On d 30, 1 SARA group (SARA-LPS) and the CON group (CON-LPS) were intramammarily challenged with a single dose of 50 µg of LPS from Escherichia coli O26:B6, whereas the remaining 6 SARA cows (SARA-PLA) received a placebo.
33663865	3	11	from	effects	633:639	arg1	fermentation					677:688	fermentation	677:688	fermentation	677:688	Therefore, the aims of this study were to determine the effects of an intermittent high-grain SARA model on the hindgut microbial community, and to evaluate whether the effects of SARA on the fecal microbiome and fermentation were further affected by an intramammary lipopolysaccharide (LPS) challenge.
33663865	3	11	from	effects	633:639	arg1	microbiome					662:671	the fecal microbiome	652:671	the fecal microbiome	652:671	Therefore, the aims of this study were to determine the effects of an intermittent high-grain SARA model on the hindgut microbial community, and to evaluate whether the effects of SARA on the fecal microbiome and fermentation were further affected by an intramammary lipopolysaccharide (LPS) challenge.
33663865	3	12	theme	lipopolysaccharide	731:748	arg1	challenge					756:764	an intramammary lipopolysaccharide (LPS) challenge	715:764	an intramammary lipopolysaccharide (LPS) challenge	715:764	Therefore, the aims of this study were to determine the effects of an intermittent high-grain SARA model on the hindgut microbial community, and to evaluate whether the effects of SARA on the fecal microbiome and fermentation were further affected by an intramammary lipopolysaccharide (LPS) challenge.
33663865	3	13	theme	model	563:567	arg1	effects					520:526	the effects	516:526	the effects of an intermittent high-grain SARA model on the hindgut microbial community	516:602	Therefore, the aims of this study were to determine the effects of an intermittent high-grain SARA model on the hindgut microbial community, and to evaluate whether the effects of SARA on the fecal microbiome and fermentation were further affected by an intramammary lipopolysaccharide (LPS) challenge.
33663865	5	14	from	Escherichia	1092:1102	arg1	LPS					1083:1085	LPS	1083:1085	LPS from Escherichia coli O26:B6	1083:1114	On d 30, 1 SARA group (SARA-LPS) and the CON group (CON-LPS) were intramammarily challenged with a single dose of 50 µg of LPS from Escherichia coli O26:B6, whereas the remaining 6 SARA cows (SARA-PLA) received a placebo.
33663865	5	14	from	Escherichia	1092:1102	arg1	µg					1077:1078	50 µg	1074:1078	50 µg of LPS from Escherichia coli O26:B6	1074:1114	On d 30, 1 SARA group (SARA-LPS) and the CON group (CON-LPS) were intramammarily challenged with a single dose of 50 µg of LPS from Escherichia coli O26:B6, whereas the remaining 6 SARA cows (SARA-PLA) received a placebo.
33663865	3	15	theme	high-grain	547:556	arg1	model					563:567	an intermittent high-grain SARA model	531:567	an intermittent high-grain SARA model	531:567	Therefore, the aims of this study were to determine the effects of an intermittent high-grain SARA model on the hindgut microbial community, and to evaluate whether the effects of SARA on the fecal microbiome and fermentation were further affected by an intramammary lipopolysaccharide (LPS) challenge.
33663865	6	16	theme	statistical	1286:1296	arg1	analysis					1298:1305	statistical analysis	1286:1305	statistical analysis	1286:1305	Using a longitudinal randomized controlled design, with grouping according to parity and days in milk), statistical analysis was performed with baseline measurements used as a covariate in a mixed model procedure.
33663865	1	17	theme	ruminal	229:235	arg1	SARA					247:250	SARA	247:250	SARA	247:250	Feeding grain-rich diets often results in subacute ruminal acidosis (SARA), a condition associated with ruminal dysbiosis and systemic inflammation.
33663865	1	17	theme	ruminal	229:235	arg1	acidosis					237:244	subacute ruminal acidosis	220:244	subacute ruminal acidosis (SARA)	220:251	Feeding grain-rich diets often results in subacute ruminal acidosis (SARA), a condition associated with ruminal dysbiosis and systemic inflammation.
33663865	1	17	theme	ruminal	229:235	arg1	condition					256:264	a condition	254:264	a condition associated with ruminal dysbiosis and systemic inflammation	254:324	Feeding grain-rich diets often results in subacute ruminal acidosis (SARA), a condition associated with ruminal dysbiosis and systemic inflammation.
33663865	4	18	theme	feeding	875:881	arg1	regimen					883:889	a SARA-inducing feeding regimen	859:889	a SARA-inducing feeding regimen (60% concentrate)	859:907	A total of 18 early-lactating Simmental cows were divided into 3 groups (n = 6); 2 were fed a SARA-inducing feeding regimen (60% concentrate), 1 was fed a control (CON) diet (40% concentrate).
33663865	4	18	theme	feeding	875:881	arg1	concentrate					896:906	60% concentrate	892:906	60% concentrate	892:906	A total of 18 early-lactating Simmental cows were divided into 3 groups (n = 6); 2 were fed a SARA-inducing feeding regimen (60% concentrate), 1 was fed a control (CON) diet (40% concentrate).
33663865	9	19	theme	richness	1956:1963	arg1	index					1965:1969	the Chao1 richness index	1946:1969	the Chao1 richness index in an interaction with the SARA challenge	1946:2011	The LPS challenge did not affect the fecal pH and short-chain fatty acids, but increased the Chao1 richness index in an interaction with the SARA challenge, and affected the relative abundance of Verrucomicrobia (1.13%), Actinobacteria (0.19%), and Spirochaetes (0.002%), suggesting an effect on the microbial ecology of the hindgut during SARA conditions.
33663865	0	20	theme	subacute	151:158	arg1	acidosis					168:175	intermittent subacute ruminal acidosis	138:175	intermittent subacute ruminal acidosis	138:175	Effect of an intramammary lipopolysaccharide challenge on the hindgut microbial composition and fermentation of dairy cattle experiencing intermittent subacute ruminal acidosis.
33663865	3	21	theme	microbial	584:592	arg1	community					594:602	the hindgut microbial community	572:602	the hindgut microbial community	572:602	Therefore, the aims of this study were to determine the effects of an intermittent high-grain SARA model on the hindgut microbial community, and to evaluate whether the effects of SARA on the fecal microbiome and fermentation were further affected by an intramammary lipopolysaccharide (LPS) challenge.
33663865	6	22	theme	model	1379:1383	arg1	procedure					1385:1393	a mixed model procedure	1371:1393	a mixed model procedure	1371:1393	Using a longitudinal randomized controlled design, with grouping according to parity and days in milk), statistical analysis was performed with baseline measurements used as a covariate in a mixed model procedure.
33663865	7	23	theme	SARA-inducing	1400:1412	arg1	challenge					1422:1430	The SARA-inducing feeding challenge	1396:1430	The SARA-inducing feeding challenge	1396:1430	The SARA-inducing feeding challenge resulted in decreased fecal pH and increased butyrate as a proportion of total short-chain fatty acids in the feces.
33663865	10	24	theme	LPS	2390:2392	arg1	challenge					2394:2402	the external LPS challenge	2377:2402	the external LPS challenge	2377:2402	In conclusion, the SARA-inducing feeding regimen promoted important microbial changes at d 30, including reduced diversity and evenness compared with CON, whereas the external LPS challenge led to changes in the microbial community without affecting fecal fermentation properties.
33663865	8	25	theme	Euryarchaeota	1695:1707	arg1	abundance					1682:1690	the relative abundance	1669:1690	the relative abundance of Euryarchaeota and cellulolytic genera	1669:1731	On d 30, SARA-challenged cows had decreased fecal diversity as shown by the Shannon and Chao1 indices and a decrease in the relative abundance of Euryarchaeota and cellulolytic genera, and numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation.
33663865	8	25	theme	Euryarchaeota	1695:1707	arg1	increases					1748:1756	numerical increases	1738:1756	numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation	1738:1854	On d 30, SARA-challenged cows had decreased fecal diversity as shown by the Shannon and Chao1 indices and a decrease in the relative abundance of Euryarchaeota and cellulolytic genera, and numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation.
33663865	10	26	theme	SARA-inducing	2233:2245	arg1	regimen					2255:2261	the SARA-inducing feeding regimen	2229:2261	the SARA-inducing feeding regimen	2229:2261	In conclusion, the SARA-inducing feeding regimen promoted important microbial changes at d 30, including reduced diversity and evenness compared with CON, whereas the external LPS challenge led to changes in the microbial community without affecting fecal fermentation properties.
33663865	8	27	theme	cellulolytic	1713:1724	arg1	genera					1726:1731	cellulolytic genera	1713:1731	cellulolytic genera	1713:1731	On d 30, SARA-challenged cows had decreased fecal diversity as shown by the Shannon and Chao1 indices and a decrease in the relative abundance of Euryarchaeota and cellulolytic genera, and numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation.
33663865	7	28	theme	fatty	1523:1527	arg1	acids					1529:1533	total short-chain fatty acids	1505:1533	total short-chain fatty acids in the feces	1505:1546	The SARA-inducing feeding challenge resulted in decreased fecal pH and increased butyrate as a proportion of total short-chain fatty acids in the feces.
33663865	5	29	dep	challenged	1041:1050	arg1	whereas					1117:1123	whereas	1117:1123	whereas	1117:1123	On d 30, 1 SARA group (SARA-LPS) and the CON group (CON-LPS) were intramammarily challenged with a single dose of 50 µg of LPS from Escherichia coli O26:B6, whereas the remaining 6 SARA cows (SARA-PLA) received a placebo.
33663865	9	30	theme	Verrucomicrobia	2053:2067	arg1	abundance					2040:2048	the relative abundance	2027:2048	the relative abundance of Verrucomicrobia (1.13%), Actinobacteria (0.19%), and Spirochaetes (0.002%)	2027:2126	The LPS challenge did not affect the fecal pH and short-chain fatty acids, but increased the Chao1 richness index in an interaction with the SARA challenge, and affected the relative abundance of Verrucomicrobia (1.13%), Actinobacteria (0.19%), and Spirochaetes (0.002%), suggesting an effect on the microbial ecology of the hindgut during SARA conditions.
33663865	0	31	theme	hindgut	62:68	arg1	composition					80:90	hindgut microbial composition	62:90	hindgut microbial composition	62:90	Effect of an intramammary lipopolysaccharide challenge on the hindgut microbial composition and fermentation of dairy cattle experiencing intermittent subacute ruminal acidosis.
33663865	7	32	theme	total	1505:1509	arg1	acids					1529:1533	total short-chain fatty acids	1505:1533	total short-chain fatty acids in the feces	1505:1546	The SARA-inducing feeding challenge resulted in decreased fecal pH and increased butyrate as a proportion of total short-chain fatty acids in the feces.
33663865	10	33	theme	important	2272:2280	arg1	changes					2292:2298	important microbial changes	2272:2298	important microbial changes	2272:2298	In conclusion, the SARA-inducing feeding regimen promoted important microbial changes at d 30, including reduced diversity and evenness compared with CON, whereas the external LPS challenge led to changes in the microbial community without affecting fecal fermentation properties.
33663865	3	34	from	effects	520:526	arg1	community					594:602	the hindgut microbial community	572:602	the hindgut microbial community	572:602	Therefore, the aims of this study were to determine the effects of an intermittent high-grain SARA model on the hindgut microbial community, and to evaluate whether the effects of SARA on the fecal microbiome and fermentation were further affected by an intramammary lipopolysaccharide (LPS) challenge.
33663865	8	35	theme	numerical	1738:1746	arg1	increases					1748:1756	numerical increases	1738:1756	numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation	1738:1854	On d 30, SARA-challenged cows had decreased fecal diversity as shown by the Shannon and Chao1 indices and a decrease in the relative abundance of Euryarchaeota and cellulolytic genera, and numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation.
33663865	8	36	theme	decreased	1583:1591	arg1	diversity					1599:1607	decreased fecal diversity	1583:1607	decreased fecal diversity	1583:1607	On d 30, SARA-challenged cows had decreased fecal diversity as shown by the Shannon and Chao1 indices and a decrease in the relative abundance of Euryarchaeota and cellulolytic genera, and numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation.
33663865	1	37	theme	grain-rich	186:195	arg1	diets					197:201	grain-rich diets	186:201	grain-rich diets	186:201	Feeding grain-rich diets often results in subacute ruminal acidosis (SARA), a condition associated with ruminal dysbiosis and systemic inflammation.
33663865	0	38	from	Effect	0:5	arg1	composition					80:90	hindgut microbial composition	62:90	hindgut microbial composition	62:90	Effect of an intramammary lipopolysaccharide challenge on the hindgut microbial composition and fermentation of dairy cattle experiencing intermittent subacute ruminal acidosis.
33663865	0	38	from	Effect	0:5	arg1	fermentation					96:107	fermentation	96:107	fermentation	96:107	Effect of an intramammary lipopolysaccharide challenge on the hindgut microbial composition and fermentation of dairy cattle experiencing intermittent subacute ruminal acidosis.
33663865	4	39	theme	%	894:894	arg1	regimen					883:889	a SARA-inducing feeding regimen	859:889	a SARA-inducing feeding regimen (60% concentrate)	859:907	A total of 18 early-lactating Simmental cows were divided into 3 groups (n = 6); 2 were fed a SARA-inducing feeding regimen (60% concentrate), 1 was fed a control (CON) diet (40% concentrate).
33663865	4	39	theme	%	894:894	arg1	concentrate					896:906	60% concentrate	892:906	60% concentrate	892:906	A total of 18 early-lactating Simmental cows were divided into 3 groups (n = 6); 2 were fed a SARA-inducing feeding regimen (60% concentrate), 1 was fed a control (CON) diet (40% concentrate).
33663865	0	40	theme	dairy	112:116	arg1	cattle					118:123	dairy cattle	112:123	dairy cattle experiencing intermittent subacute ruminal acidosis	112:175	Effect of an intramammary lipopolysaccharide challenge on the hindgut microbial composition and fermentation of dairy cattle experiencing intermittent subacute ruminal acidosis.
33663865	0	41	theme	challenge	45:53	arg1	Effect					0:5	Effect	0:5	Effect of an intramammary lipopolysaccharide challenge on the hindgut microbial composition and fermentation of dairy cattle experiencing intermittent subacute ruminal acidosis.	0:176	Effect of an intramammary lipopolysaccharide challenge on the hindgut microbial composition and fermentation of dairy cattle experiencing intermittent subacute ruminal acidosis.
33663865	7	42	theme	increased	1467:1475	arg1	butyrate					1477:1484	increased butyrate	1467:1484	increased butyrate	1467:1484	The SARA-inducing feeding challenge resulted in decreased fecal pH and increased butyrate as a proportion of total short-chain fatty acids in the feces.
33663865	4	43	theme	CON	931:933	arg1	concentrate					946:956	40% concentrate	942:956	40% concentrate	942:956	A total of 18 early-lactating Simmental cows were divided into 3 groups (n = 6); 2 were fed a SARA-inducing feeding regimen (60% concentrate), 1 was fed a control (CON) diet (40% concentrate).
33663865	4	43	theme	CON	931:933	arg1	diet					936:939	a control (CON) diet	920:939	a control (CON) diet (40% concentrate)	920:957	A total of 18 early-lactating Simmental cows were divided into 3 groups (n = 6); 2 were fed a SARA-inducing feeding regimen (60% concentrate), 1 was fed a control (CON) diet (40% concentrate).
33663865	10	44	theme	fermentation	2470:2481	arg1	properties					2483:2492	fecal fermentation properties	2464:2492	fecal fermentation properties	2464:2492	In conclusion, the SARA-inducing feeding regimen promoted important microbial changes at d 30, including reduced diversity and evenness compared with CON, whereas the external LPS challenge led to changes in the microbial community without affecting fecal fermentation properties.
33663865	0	45	dep	composition	80:90	arg1	the					58:60	the	58:60	the	58:60	Effect of an intramammary lipopolysaccharide challenge on the hindgut microbial composition and fermentation of dairy cattle experiencing intermittent subacute ruminal acidosis.
33663865	10	46	theme	reduced	2319:2325	arg1	diversity					2327:2335	diversity	2327:2335	diversity	2327:2335	In conclusion, the SARA-inducing feeding regimen promoted important microbial changes at d 30, including reduced diversity and evenness compared with CON, whereas the external LPS challenge led to changes in the microbial community without affecting fecal fermentation properties.
33663865	2	47	theme	immune	428:433	arg1	stimuli					435:441	exogenous immune stimuli	418:441	exogenous immune stimuli	418:441	Yet, the effect of SARA on hindgut microbiota, and whether this condition is aggravated by exogenous immune stimuli, is less understood.
33663865	8	48	theme	Firmicutes	1795:1804	arg1	abundance					1774:1782	the relative abundance	1761:1782	the relative abundance of several Firmicutes associated with starch and secondary fermentation	1761:1854	On d 30, SARA-challenged cows had decreased fecal diversity as shown by the Shannon and Chao1 indices and a decrease in the relative abundance of Euryarchaeota and cellulolytic genera, and numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation.
33663865	1	49	theme	systemic	304:311	arg1	inflammation					313:324	systemic inflammation	304:324	systemic inflammation	304:324	Feeding grain-rich diets often results in subacute ruminal acidosis (SARA), a condition associated with ruminal dysbiosis and systemic inflammation.
33663865	7	50	theme	decreased	1444:1452	arg1	pH					1460:1461	decreased fecal pH	1444:1461	decreased fecal pH	1444:1461	The SARA-inducing feeding challenge resulted in decreased fecal pH and increased butyrate as a proportion of total short-chain fatty acids in the feces.
33663865	4	51	theme	Simmental	797:805	arg1	cows					807:810	18 early-lactating Simmental cows	778:810	18 early-lactating Simmental cows	778:810	A total of 18 early-lactating Simmental cows were divided into 3 groups (n = 6); 2 were fed a SARA-inducing feeding regimen (60% concentrate), 1 was fed a control (CON) diet (40% concentrate).
33663865	6	52	theme	randomized	1203:1212	arg1	design					1225:1230	a longitudinal randomized controlled design	1188:1230	a longitudinal randomized controlled design, with grouping according to parity and days in milk)	1188:1283	Using a longitudinal randomized controlled design, with grouping according to parity and days in milk), statistical analysis was performed with baseline measurements used as a covariate in a mixed model procedure.
33663865	0	53	theme	lipopolysaccharide	26:43	arg1	challenge					45:53	an intramammary lipopolysaccharide challenge	10:53	an intramammary lipopolysaccharide challenge	10:53	Effect of an intramammary lipopolysaccharide challenge on the hindgut microbial composition and fermentation of dairy cattle experiencing intermittent subacute ruminal acidosis.
33663865	6	54	from	days	1271:1274	arg1	milk					1279:1282	milk	1279:1282	milk	1279:1282	Using a longitudinal randomized controlled design, with grouping according to parity and days in milk), statistical analysis was performed with baseline measurements used as a covariate in a mixed model procedure.
33663865	4	55	theme	control	922:928	arg1	concentrate					946:956	40% concentrate	942:956	40% concentrate	942:956	A total of 18 early-lactating Simmental cows were divided into 3 groups (n = 6); 2 were fed a SARA-inducing feeding regimen (60% concentrate), 1 was fed a control (CON) diet (40% concentrate).
33663865	4	55	theme	control	922:928	arg1	diet					936:939	a control (CON) diet	920:939	a control (CON) diet (40% concentrate)	920:957	A total of 18 early-lactating Simmental cows were divided into 3 groups (n = 6); 2 were fed a SARA-inducing feeding regimen (60% concentrate), 1 was fed a control (CON) diet (40% concentrate).
33663865	8	56	from	decrease	1657:1664	arg1	abundance					1682:1690	the relative abundance	1669:1690	the relative abundance of Euryarchaeota and cellulolytic genera	1669:1731	On d 30, SARA-challenged cows had decreased fecal diversity as shown by the Shannon and Chao1 indices and a decrease in the relative abundance of Euryarchaeota and cellulolytic genera, and numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation.
33663865	8	56	from	decrease	1657:1664	arg1	increases					1748:1756	numerical increases	1738:1756	numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation	1738:1854	On d 30, SARA-challenged cows had decreased fecal diversity as shown by the Shannon and Chao1 indices and a decrease in the relative abundance of Euryarchaeota and cellulolytic genera, and numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation.
33663865	9	57	theme	LPS	1861:1863	arg1	challenge					1865:1873	The LPS challenge	1857:1873	The LPS challenge	1857:1873	The LPS challenge did not affect the fecal pH and short-chain fatty acids, but increased the Chao1 richness index in an interaction with the SARA challenge, and affected the relative abundance of Verrucomicrobia (1.13%), Actinobacteria (0.19%), and Spirochaetes (0.002%), suggesting an effect on the microbial ecology of the hindgut during SARA conditions.
33663865	5	58	theme	CON	1001:1003	arg1	CON-LPS					1012:1018	CON-LPS	1012:1018	CON-LPS	1012:1018	On d 30, 1 SARA group (SARA-LPS) and the CON group (CON-LPS) were intramammarily challenged with a single dose of 50 µg of LPS from Escherichia coli O26:B6, whereas the remaining 6 SARA cows (SARA-PLA) received a placebo.
33663865	5	58	theme	CON	1001:1003	arg1	group					1005:1009	the CON group	997:1009	the CON group (CON-LPS)	997:1019	On d 30, 1 SARA group (SARA-LPS) and the CON group (CON-LPS) were intramammarily challenged with a single dose of 50 µg of LPS from Escherichia coli O26:B6, whereas the remaining 6 SARA cows (SARA-PLA) received a placebo.
33663865	10	59	theme	external	2381:2388	arg1	challenge					2394:2402	the external LPS challenge	2377:2402	the external LPS challenge	2377:2402	In conclusion, the SARA-inducing feeding regimen promoted important microbial changes at d 30, including reduced diversity and evenness compared with CON, whereas the external LPS challenge led to changes in the microbial community without affecting fecal fermentation properties.
33663865	2	60	dep	aggravated	404:413	arg1	understood					452:461	understood	452:461	is less understood	444:461	Yet, the effect of SARA on hindgut microbiota, and whether this condition is aggravated by exogenous immune stimuli, is less understood.
33663865	9	61	theme	Actinobacteria	2078:2091	arg1	abundance					2040:2048	the relative abundance	2027:2048	the relative abundance of Verrucomicrobia (1.13%), Actinobacteria (0.19%), and Spirochaetes (0.002%)	2027:2126	The LPS challenge did not affect the fecal pH and short-chain fatty acids, but increased the Chao1 richness index in an interaction with the SARA challenge, and affected the relative abundance of Verrucomicrobia (1.13%), Actinobacteria (0.19%), and Spirochaetes (0.002%), suggesting an effect on the microbial ecology of the hindgut during SARA conditions.
33663865	5	62	theme	single	1059:1064	arg1	dose					1066:1069	a single dose	1057:1069	a single dose of 50 µg of LPS from Escherichia coli O26:B6	1057:1114	On d 30, 1 SARA group (SARA-LPS) and the CON group (CON-LPS) were intramammarily challenged with a single dose of 50 µg of LPS from Escherichia coli O26:B6, whereas the remaining 6 SARA cows (SARA-PLA) received a placebo.
33663865	8	63	from	abundance	1682:1690	arg1	abundance					1774:1782	the relative abundance	1761:1782	the relative abundance of several Firmicutes associated with starch and secondary fermentation	1761:1854	On d 30, SARA-challenged cows had decreased fecal diversity as shown by the Shannon and Chao1 indices and a decrease in the relative abundance of Euryarchaeota and cellulolytic genera, and numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation.
33663865	3	64	theme	SARA	644:647	arg1	effects					633:639	the effects	629:639	the effects of SARA on the fecal microbiome and fermentation	629:688	Therefore, the aims of this study were to determine the effects of an intermittent high-grain SARA model on the hindgut microbial community, and to evaluate whether the effects of SARA on the fecal microbiome and fermentation were further affected by an intramammary lipopolysaccharide (LPS) challenge.
33663865	8	65	theme	secondary	1833:1841	arg1	fermentation					1843:1854	starch and secondary fermentation	1822:1854	starch and secondary fermentation	1822:1854	On d 30, SARA-challenged cows had decreased fecal diversity as shown by the Shannon and Chao1 indices and a decrease in the relative abundance of Euryarchaeota and cellulolytic genera, and numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation.
33663865	6	66	from	parity	1260:1265	arg1	milk					1279:1282	milk	1279:1282	milk	1279:1282	Using a longitudinal randomized controlled design, with grouping according to parity and days in milk), statistical analysis was performed with baseline measurements used as a covariate in a mixed model procedure.
33663865	4	67	theme	40	942:943	arg1	%					944:944	%	944:944	%	944:944	A total of 18 early-lactating Simmental cows were divided into 3 groups (n = 6); 2 were fed a SARA-inducing feeding regimen (60% concentrate), 1 was fed a control (CON) diet (40% concentrate).
33663865	9	68	theme	fecal	1894:1898	arg1	pH					1900:1901	the fecal pH	1890:1901	the fecal pH	1890:1901	The LPS challenge did not affect the fecal pH and short-chain fatty acids, but increased the Chao1 richness index in an interaction with the SARA challenge, and affected the relative abundance of Verrucomicrobia (1.13%), Actinobacteria (0.19%), and Spirochaetes (0.002%), suggesting an effect on the microbial ecology of the hindgut during SARA conditions.
33663865	3	69	theme	study	492:496	arg1	aims					479:482	the aims	475:482	the aims of this study	475:496	Therefore, the aims of this study were to determine the effects of an intermittent high-grain SARA model on the hindgut microbial community, and to evaluate whether the effects of SARA on the fecal microbiome and fermentation were further affected by an intramammary lipopolysaccharide (LPS) challenge.
33663865	9	70	theme	Chao1	1950:1954	arg1	index					1965:1969	the Chao1 richness index	1946:1969	the Chao1 richness index in an interaction with the SARA challenge	1946:2011	The LPS challenge did not affect the fecal pH and short-chain fatty acids, but increased the Chao1 richness index in an interaction with the SARA challenge, and affected the relative abundance of Verrucomicrobia (1.13%), Actinobacteria (0.19%), and Spirochaetes (0.002%), suggesting an effect on the microbial ecology of the hindgut during SARA conditions.
33663865	8	71	contain	had	1579:1581	arg2	diversity					1599:1607	decreased fecal diversity	1583:1607	decreased fecal diversity	1583:1607	On d 30, SARA-challenged cows had decreased fecal diversity as shown by the Shannon and Chao1 indices and a decrease in the relative abundance of Euryarchaeota and cellulolytic genera, and numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation.
33663865	8	71	contain	had	1579:1581	arg1	cows					1574:1577	SARA-challenged cows	1558:1577	SARA-challenged cows	1558:1577	On d 30, SARA-challenged cows had decreased fecal diversity as shown by the Shannon and Chao1 indices and a decrease in the relative abundance of Euryarchaeota and cellulolytic genera, and numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation.
33663865	4	72	theme	=	842:842	arg1	n					840:840	n = 6	840:844	n = 6	840:844	A total of 18 early-lactating Simmental cows were divided into 3 groups (n = 6); 2 were fed a SARA-inducing feeding regimen (60% concentrate), 1 was fed a control (CON) diet (40% concentrate).
33663865	4	72	theme	=	842:842	arg1	groups					832:837	3 groups	830:837	3 groups (n = 6)	830:845	A total of 18 early-lactating Simmental cows were divided into 3 groups (n = 6); 2 were fed a SARA-inducing feeding regimen (60% concentrate), 1 was fed a control (CON) diet (40% concentrate).
33663865	5	73	dep	Escherichia	1092:1102	arg1	coli					1104:1107	coli	1104:1107	coli	1104:1107	On d 30, 1 SARA group (SARA-LPS) and the CON group (CON-LPS) were intramammarily challenged with a single dose of 50 µg of LPS from Escherichia coli O26:B6, whereas the remaining 6 SARA cows (SARA-PLA) received a placebo.
33663865	5	73	dep	Escherichia	1092:1102	arg1	B6					1113:1114	B6	1113:1114	B6	1113:1114	On d 30, 1 SARA group (SARA-LPS) and the CON group (CON-LPS) were intramammarily challenged with a single dose of 50 µg of LPS from Escherichia coli O26:B6, whereas the remaining 6 SARA cows (SARA-PLA) received a placebo.
33663865	5	73	dep	Escherichia	1092:1102	arg1	O26					1109:1111	O26	1109:1111	O26	1109:1111	On d 30, 1 SARA group (SARA-LPS) and the CON group (CON-LPS) were intramammarily challenged with a single dose of 50 µg of LPS from Escherichia coli O26:B6, whereas the remaining 6 SARA cows (SARA-PLA) received a placebo.
33663865	9	74	theme	fatty	1919:1923	arg1	acids					1925:1929	short-chain fatty acids	1907:1929	short-chain fatty acids	1907:1929	The LPS challenge did not affect the fecal pH and short-chain fatty acids, but increased the Chao1 richness index in an interaction with the SARA challenge, and affected the relative abundance of Verrucomicrobia (1.13%), Actinobacteria (0.19%), and Spirochaetes (0.002%), suggesting an effect on the microbial ecology of the hindgut during SARA conditions.
33663865	6	75	theme	baseline	1326:1333	arg1	measurements					1335:1346	baseline measurements	1326:1346	baseline measurements used as a covariate in a mixed model procedure	1326:1393	Using a longitudinal randomized controlled design, with grouping according to parity and days in milk), statistical analysis was performed with baseline measurements used as a covariate in a mixed model procedure.
33663865	9	76	from	index	1965:1969	arg1	interaction					1977:1987	an interaction	1974:1987	an interaction with the SARA challenge	1974:2011	The LPS challenge did not affect the fecal pH and short-chain fatty acids, but increased the Chao1 richness index in an interaction with the SARA challenge, and affected the relative abundance of Verrucomicrobia (1.13%), Actinobacteria (0.19%), and Spirochaetes (0.002%), suggesting an effect on the microbial ecology of the hindgut during SARA conditions.
33663865	3	77	theme	SARA	558:561	arg1	model					563:567	an intermittent high-grain SARA model	531:567	an intermittent high-grain SARA model	531:567	Therefore, the aims of this study were to determine the effects of an intermittent high-grain SARA model on the hindgut microbial community, and to evaluate whether the effects of SARA on the fecal microbiome and fermentation were further affected by an intramammary lipopolysaccharide (LPS) challenge.
33663865	7	78	from	acids	1529:1533	arg1	feces					1542:1546	the feces	1538:1546	the feces	1538:1546	The SARA-inducing feeding challenge resulted in decreased fecal pH and increased butyrate as a proportion of total short-chain fatty acids in the feces.
33663865	0	79	theme	intermittent	138:149	arg1	acidosis					168:175	intermittent subacute ruminal acidosis	138:175	intermittent subacute ruminal acidosis	138:175	Effect of an intramammary lipopolysaccharide challenge on the hindgut microbial composition and fermentation of dairy cattle experiencing intermittent subacute ruminal acidosis.
33663865	3	80	theme	intermittent	534:545	arg1	model					563:567	an intermittent high-grain SARA model	531:567	an intermittent high-grain SARA model	531:567	Therefore, the aims of this study were to determine the effects of an intermittent high-grain SARA model on the hindgut microbial community, and to evaluate whether the effects of SARA on the fecal microbiome and fermentation were further affected by an intramammary lipopolysaccharide (LPS) challenge.
33663865	1	81	theme	subacute	220:227	arg1	SARA					247:250	SARA	247:250	SARA	247:250	Feeding grain-rich diets often results in subacute ruminal acidosis (SARA), a condition associated with ruminal dysbiosis and systemic inflammation.
33663865	1	81	theme	subacute	220:227	arg1	acidosis					237:244	subacute ruminal acidosis	220:244	subacute ruminal acidosis (SARA)	220:251	Feeding grain-rich diets often results in subacute ruminal acidosis (SARA), a condition associated with ruminal dysbiosis and systemic inflammation.
33663865	1	81	theme	subacute	220:227	arg1	condition					256:264	a condition	254:264	a condition associated with ruminal dysbiosis and systemic inflammation	254:324	Feeding grain-rich diets often results in subacute ruminal acidosis (SARA), a condition associated with ruminal dysbiosis and systemic inflammation.
33663865	7	82	theme	feeding	1414:1420	arg1	challenge					1422:1430	The SARA-inducing feeding challenge	1396:1430	The SARA-inducing feeding challenge	1396:1430	The SARA-inducing feeding challenge resulted in decreased fecal pH and increased butyrate as a proportion of total short-chain fatty acids in the feces.
33663865	8	83	from	increases	1748:1756	arg1	abundance					1774:1782	the relative abundance	1761:1782	the relative abundance of several Firmicutes associated with starch and secondary fermentation	1761:1854	On d 30, SARA-challenged cows had decreased fecal diversity as shown by the Shannon and Chao1 indices and a decrease in the relative abundance of Euryarchaeota and cellulolytic genera, and numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation.
33663865	3	84	theme	intramammary	718:729	arg1	challenge					756:764	an intramammary lipopolysaccharide (LPS) challenge	715:764	an intramammary lipopolysaccharide (LPS) challenge	715:764	Therefore, the aims of this study were to determine the effects of an intermittent high-grain SARA model on the hindgut microbial community, and to evaluate whether the effects of SARA on the fecal microbiome and fermentation were further affected by an intramammary lipopolysaccharide (LPS) challenge.
33663865	0	85	theme	ruminal	160:166	arg1	acidosis					168:175	intermittent subacute ruminal acidosis	138:175	intermittent subacute ruminal acidosis	138:175	Effect of an intramammary lipopolysaccharide challenge on the hindgut microbial composition and fermentation of dairy cattle experiencing intermittent subacute ruminal acidosis.
33663865	3	86	theme	hindgut	576:582	arg1	community					594:602	the hindgut microbial community	572:602	the hindgut microbial community	572:602	Therefore, the aims of this study were to determine the effects of an intermittent high-grain SARA model on the hindgut microbial community, and to evaluate whether the effects of SARA on the fecal microbiome and fermentation were further affected by an intramammary lipopolysaccharide (LPS) challenge.
33663865	9	87	from	effect	2143:2148	arg1	ecology					2167:2173	the microbial ecology	2153:2173	the microbial ecology of the hindgut	2153:2188	The LPS challenge did not affect the fecal pH and short-chain fatty acids, but increased the Chao1 richness index in an interaction with the SARA challenge, and affected the relative abundance of Verrucomicrobia (1.13%), Actinobacteria (0.19%), and Spirochaetes (0.002%), suggesting an effect on the microbial ecology of the hindgut during SARA conditions.
33663865	4	88	theme	SARA-inducing	861:873	arg1	regimen					883:889	a SARA-inducing feeding regimen	859:889	a SARA-inducing feeding regimen (60% concentrate)	859:907	A total of 18 early-lactating Simmental cows were divided into 3 groups (n = 6); 2 were fed a SARA-inducing feeding regimen (60% concentrate), 1 was fed a control (CON) diet (40% concentrate).
33663865	4	88	theme	SARA-inducing	861:873	arg1	concentrate					896:906	60% concentrate	892:906	60% concentrate	892:906	A total of 18 early-lactating Simmental cows were divided into 3 groups (n = 6); 2 were fed a SARA-inducing feeding regimen (60% concentrate), 1 was fed a control (CON) diet (40% concentrate).
33663865	6	89	theme	mixed	1373:1377	arg1	procedure					1385:1393	a mixed model procedure	1371:1393	a mixed model procedure	1371:1393	Using a longitudinal randomized controlled design, with grouping according to parity and days in milk), statistical analysis was performed with baseline measurements used as a covariate in a mixed model procedure.
33663865	8	90	theme	relative	1673:1680	arg1	abundance					1682:1690	the relative abundance	1669:1690	the relative abundance of Euryarchaeota and cellulolytic genera	1669:1731	On d 30, SARA-challenged cows had decreased fecal diversity as shown by the Shannon and Chao1 indices and a decrease in the relative abundance of Euryarchaeota and cellulolytic genera, and numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation.
33663865	10	91	theme	microbial	2426:2434	arg1	community					2436:2444	the microbial community	2422:2444	the microbial community	2422:2444	In conclusion, the SARA-inducing feeding regimen promoted important microbial changes at d 30, including reduced diversity and evenness compared with CON, whereas the external LPS challenge led to changes in the microbial community without affecting fecal fermentation properties.
33663865	10	92	theme	feeding	2247:2253	arg1	regimen					2255:2261	the SARA-inducing feeding regimen	2229:2261	the SARA-inducing feeding regimen	2229:2261	In conclusion, the SARA-inducing feeding regimen promoted important microbial changes at d 30, including reduced diversity and evenness compared with CON, whereas the external LPS challenge led to changes in the microbial community without affecting fecal fermentation properties.
33663865	8	93	theme	genera	1726:1731	arg1	abundance					1682:1690	the relative abundance	1669:1690	the relative abundance of Euryarchaeota and cellulolytic genera	1669:1731	On d 30, SARA-challenged cows had decreased fecal diversity as shown by the Shannon and Chao1 indices and a decrease in the relative abundance of Euryarchaeota and cellulolytic genera, and numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation.
33663865	8	93	theme	genera	1726:1731	arg1	increases					1748:1756	numerical increases	1738:1756	numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation	1738:1854	On d 30, SARA-challenged cows had decreased fecal diversity as shown by the Shannon and Chao1 indices and a decrease in the relative abundance of Euryarchaeota and cellulolytic genera, and numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation.
33663865	9	94	theme	microbial	2157:2165	arg1	ecology					2167:2173	the microbial ecology	2153:2173	the microbial ecology of the hindgut	2153:2188	The LPS challenge did not affect the fecal pH and short-chain fatty acids, but increased the Chao1 richness index in an interaction with the SARA challenge, and affected the relative abundance of Verrucomicrobia (1.13%), Actinobacteria (0.19%), and Spirochaetes (0.002%), suggesting an effect on the microbial ecology of the hindgut during SARA conditions.
33663865	0	95	theme	microbial	70:78	arg1	composition					80:90	hindgut microbial composition	62:90	hindgut microbial composition	62:90	Effect of an intramammary lipopolysaccharide challenge on the hindgut microbial composition and fermentation of dairy cattle experiencing intermittent subacute ruminal acidosis.
33663865	7	96	theme	acids	1529:1533	arg1	proportion					1491:1500	a proportion	1489:1500	a proportion of total short-chain fatty acids in the feces	1489:1546	The SARA-inducing feeding challenge resulted in decreased fecal pH and increased butyrate as a proportion of total short-chain fatty acids in the feces.
33663865	10	97	theme	fecal	2464:2468	arg1	properties					2483:2492	fecal fermentation properties	2464:2492	fecal fermentation properties	2464:2492	In conclusion, the SARA-inducing feeding regimen promoted important microbial changes at d 30, including reduced diversity and evenness compared with CON, whereas the external LPS challenge led to changes in the microbial community without affecting fecal fermentation properties.
33663865	9	98	with	interaction	1977:1987	arg1	challenge					2003:2011	the SARA challenge	1994:2011	the SARA challenge	1994:2011	The LPS challenge did not affect the fecal pH and short-chain fatty acids, but increased the Chao1 richness index in an interaction with the SARA challenge, and affected the relative abundance of Verrucomicrobia (1.13%), Actinobacteria (0.19%), and Spirochaetes (0.002%), suggesting an effect on the microbial ecology of the hindgut during SARA conditions.
33663865	10	99	theme	microbial	2282:2290	arg1	changes					2292:2298	important microbial changes	2272:2298	important microbial changes	2272:2298	In conclusion, the SARA-inducing feeding regimen promoted important microbial changes at d 30, including reduced diversity and evenness compared with CON, whereas the external LPS challenge led to changes in the microbial community without affecting fecal fermentation properties.
33663865	5	100	theme	µg	1077:1078	arg1	dose					1066:1069	a single dose	1057:1069	a single dose of 50 µg of LPS from Escherichia coli O26:B6	1057:1114	On d 30, 1 SARA group (SARA-LPS) and the CON group (CON-LPS) were intramammarily challenged with a single dose of 50 µg of LPS from Escherichia coli O26:B6, whereas the remaining 6 SARA cows (SARA-PLA) received a placebo.
33663865	9	101	theme	SARA	1998:2001	arg1	challenge					2003:2011	the SARA challenge	1994:2011	the SARA challenge	1994:2011	The LPS challenge did not affect the fecal pH and short-chain fatty acids, but increased the Chao1 richness index in an interaction with the SARA challenge, and affected the relative abundance of Verrucomicrobia (1.13%), Actinobacteria (0.19%), and Spirochaetes (0.002%), suggesting an effect on the microbial ecology of the hindgut during SARA conditions.
33663865	9	102	theme	hindgut	2182:2188	arg1	ecology					2167:2173	the microbial ecology	2153:2173	the microbial ecology of the hindgut	2153:2188	The LPS challenge did not affect the fecal pH and short-chain fatty acids, but increased the Chao1 richness index in an interaction with the SARA challenge, and affected the relative abundance of Verrucomicrobia (1.13%), Actinobacteria (0.19%), and Spirochaetes (0.002%), suggesting an effect on the microbial ecology of the hindgut during SARA conditions.
33663865	8	103	theme	SARA-challenged	1558:1572	arg1	cows					1574:1577	SARA-challenged cows	1558:1577	SARA-challenged cows	1558:1577	On d 30, SARA-challenged cows had decreased fecal diversity as shown by the Shannon and Chao1 indices and a decrease in the relative abundance of Euryarchaeota and cellulolytic genera, and numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation.
33663865	6	104	from	covariate	1358:1366	arg1	procedure					1385:1393	a mixed model procedure	1371:1393	a mixed model procedure	1371:1393	Using a longitudinal randomized controlled design, with grouping according to parity and days in milk), statistical analysis was performed with baseline measurements used as a covariate in a mixed model procedure.
33663865	7	105	theme	short-chain	1511:1521	arg1	acids					1529:1533	total short-chain fatty acids	1505:1533	total short-chain fatty acids in the feces	1505:1546	The SARA-inducing feeding challenge resulted in decreased fecal pH and increased butyrate as a proportion of total short-chain fatty acids in the feces.
33663865	5	106	theme	LPS	1083:1085	arg1	µg					1077:1078	50 µg	1074:1078	50 µg of LPS from Escherichia coli O26:B6	1074:1114	On d 30, 1 SARA group (SARA-LPS) and the CON group (CON-LPS) were intramammarily challenged with a single dose of 50 µg of LPS from Escherichia coli O26:B6, whereas the remaining 6 SARA cows (SARA-PLA) received a placebo.
33663865	9	107	theme	SARA	2197:2200	arg1	conditions					2202:2211	SARA conditions	2197:2211	SARA conditions	2197:2211	The LPS challenge did not affect the fecal pH and short-chain fatty acids, but increased the Chao1 richness index in an interaction with the SARA challenge, and affected the relative abundance of Verrucomicrobia (1.13%), Actinobacteria (0.19%), and Spirochaetes (0.002%), suggesting an effect on the microbial ecology of the hindgut during SARA conditions.
33663865	6	108	with	design	1225:1230	arg1	grouping					1238:1245	grouping	1238:1245	grouping according to parity and days in milk	1238:1282	Using a longitudinal randomized controlled design, with grouping according to parity and days in milk), statistical analysis was performed with baseline measurements used as a covariate in a mixed model procedure.
33663865	10	109	from	changes	2411:2417	arg1	community					2436:2444	the microbial community	2422:2444	the microbial community	2422:2444	In conclusion, the SARA-inducing feeding regimen promoted important microbial changes at d 30, including reduced diversity and evenness compared with CON, whereas the external LPS challenge led to changes in the microbial community without affecting fecal fermentation properties.
33663865	5	110	theme	remaining	1129:1137	arg1	SARA-PLA					1152:1159	SARA-PLA	1152:1159	SARA-PLA	1152:1159	On d 30, 1 SARA group (SARA-LPS) and the CON group (CON-LPS) were intramammarily challenged with a single dose of 50 µg of LPS from Escherichia coli O26:B6, whereas the remaining 6 SARA cows (SARA-PLA) received a placebo.
33663865	5	110	theme	remaining	1129:1137	arg1	cows					1146:1149	the remaining 6 SARA cows	1125:1149	the remaining 6 SARA cows (SARA-PLA)	1125:1160	On d 30, 1 SARA group (SARA-LPS) and the CON group (CON-LPS) were intramammarily challenged with a single dose of 50 µg of LPS from Escherichia coli O26:B6, whereas the remaining 6 SARA cows (SARA-PLA) received a placebo.
33663865	0	111	theme	cattle	118:123	arg1	composition					80:90	hindgut microbial composition	62:90	hindgut microbial composition	62:90	Effect of an intramammary lipopolysaccharide challenge on the hindgut microbial composition and fermentation of dairy cattle experiencing intermittent subacute ruminal acidosis.
33663865	0	111	theme	cattle	118:123	arg1	fermentation					96:107	fermentation	96:107	fermentation	96:107	Effect of an intramammary lipopolysaccharide challenge on the hindgut microbial composition and fermentation of dairy cattle experiencing intermittent subacute ruminal acidosis.
33663865	8	112	theme	fecal	1593:1597	arg1	diversity					1599:1607	decreased fecal diversity	1583:1607	decreased fecal diversity	1583:1607	On d 30, SARA-challenged cows had decreased fecal diversity as shown by the Shannon and Chao1 indices and a decrease in the relative abundance of Euryarchaeota and cellulolytic genera, and numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation.
33663865	5	113	theme	SARA	1141:1144	arg1	SARA-PLA					1152:1159	SARA-PLA	1152:1159	SARA-PLA	1152:1159	On d 30, 1 SARA group (SARA-LPS) and the CON group (CON-LPS) were intramammarily challenged with a single dose of 50 µg of LPS from Escherichia coli O26:B6, whereas the remaining 6 SARA cows (SARA-PLA) received a placebo.
33663865	5	113	theme	SARA	1141:1144	arg1	cows					1146:1149	the remaining 6 SARA cows	1125:1149	the remaining 6 SARA cows (SARA-PLA)	1125:1160	On d 30, 1 SARA group (SARA-LPS) and the CON group (CON-LPS) were intramammarily challenged with a single dose of 50 µg of LPS from Escherichia coli O26:B6, whereas the remaining 6 SARA cows (SARA-PLA) received a placebo.
33663865	9	114	theme	relative	2031:2038	arg1	abundance					2040:2048	the relative abundance	2027:2048	the relative abundance of Verrucomicrobia (1.13%), Actinobacteria (0.19%), and Spirochaetes (0.002%)	2027:2126	The LPS challenge did not affect the fecal pH and short-chain fatty acids, but increased the Chao1 richness index in an interaction with the SARA challenge, and affected the relative abundance of Verrucomicrobia (1.13%), Actinobacteria (0.19%), and Spirochaetes (0.002%), suggesting an effect on the microbial ecology of the hindgut during SARA conditions.
33663865	8	115	theme	several	1787:1793	arg1	Firmicutes					1795:1804	several Firmicutes	1787:1804	several Firmicutes associated with starch and secondary fermentation	1787:1854	On d 30, SARA-challenged cows had decreased fecal diversity as shown by the Shannon and Chao1 indices and a decrease in the relative abundance of Euryarchaeota and cellulolytic genera, and numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation.
33663865	5	116	theme	SARA	971:974	arg1	SARA-LPS					983:990	SARA-LPS	983:990	SARA-LPS	983:990	On d 30, 1 SARA group (SARA-LPS) and the CON group (CON-LPS) were intramammarily challenged with a single dose of 50 µg of LPS from Escherichia coli O26:B6, whereas the remaining 6 SARA cows (SARA-PLA) received a placebo.
33663865	5	116	theme	SARA	971:974	arg1	group					976:980	1 SARA group	969:980	1 SARA group (SARA-LPS)	969:991	On d 30, 1 SARA group (SARA-LPS) and the CON group (CON-LPS) were intramammarily challenged with a single dose of 50 µg of LPS from Escherichia coli O26:B6, whereas the remaining 6 SARA cows (SARA-PLA) received a placebo.
33663865	8	117	from	indices	1643:1649	arg1	abundance					1682:1690	the relative abundance	1669:1690	the relative abundance of Euryarchaeota and cellulolytic genera	1669:1731	On d 30, SARA-challenged cows had decreased fecal diversity as shown by the Shannon and Chao1 indices and a decrease in the relative abundance of Euryarchaeota and cellulolytic genera, and numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation.
33663865	8	117	from	indices	1643:1649	arg1	increases					1748:1756	numerical increases	1738:1756	numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation	1738:1854	On d 30, SARA-challenged cows had decreased fecal diversity as shown by the Shannon and Chao1 indices and a decrease in the relative abundance of Euryarchaeota and cellulolytic genera, and numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation.
33663865	0	118	theme	intramammary	13:24	arg1	challenge					45:53	an intramammary lipopolysaccharide challenge	10:53	an intramammary lipopolysaccharide challenge	10:53	Effect of an intramammary lipopolysaccharide challenge on the hindgut microbial composition and fermentation of dairy cattle experiencing intermittent subacute ruminal acidosis.
33663865	2	119	theme	exogenous	418:426	arg1	stimuli					435:441	exogenous immune stimuli	418:441	exogenous immune stimuli	418:441	Yet, the effect of SARA on hindgut microbiota, and whether this condition is aggravated by exogenous immune stimuli, is less understood.
33663865	6	120	theme	controlled	1214:1223	arg1	design					1225:1230	a longitudinal randomized controlled design	1188:1230	a longitudinal randomized controlled design, with grouping according to parity and days in milk)	1188:1283	Using a longitudinal randomized controlled design, with grouping according to parity and days in milk), statistical analysis was performed with baseline measurements used as a covariate in a mixed model procedure.
33663865	7	121	theme	fecal	1454:1458	arg1	pH					1460:1461	decreased fecal pH	1444:1461	decreased fecal pH	1444:1461	The SARA-inducing feeding challenge resulted in decreased fecal pH and increased butyrate as a proportion of total short-chain fatty acids in the feces.
33663865	4	122	theme	early-lactating	781:795	arg1	cows					807:810	18 early-lactating Simmental cows	778:810	18 early-lactating Simmental cows	778:810	A total of 18 early-lactating Simmental cows were divided into 3 groups (n = 6); 2 were fed a SARA-inducing feeding regimen (60% concentrate), 1 was fed a control (CON) diet (40% concentrate).
33663865	8	123	theme	relative	1765:1772	arg1	abundance					1774:1782	the relative abundance	1761:1782	the relative abundance of several Firmicutes associated with starch and secondary fermentation	1761:1854	On d 30, SARA-challenged cows had decreased fecal diversity as shown by the Shannon and Chao1 indices and a decrease in the relative abundance of Euryarchaeota and cellulolytic genera, and numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation.
33663865	6	124	theme	longitudinal	1190:1201	arg1	design					1225:1230	a longitudinal randomized controlled design	1188:1230	a longitudinal randomized controlled design, with grouping according to parity and days in milk)	1188:1283	Using a longitudinal randomized controlled design, with grouping according to parity and days in milk), statistical analysis was performed with baseline measurements used as a covariate in a mixed model procedure.
33663865	8	125	theme	starch	1822:1827	arg1	fermentation					1843:1854	starch and secondary fermentation	1822:1854	starch and secondary fermentation	1822:1854	On d 30, SARA-challenged cows had decreased fecal diversity as shown by the Shannon and Chao1 indices and a decrease in the relative abundance of Euryarchaeota and cellulolytic genera, and numerical increases in the relative abundance of several Firmicutes associated with starch and secondary fermentation.
33663865	7	126	from	feces	1542:1546	arg1	proportion					1491:1500	a proportion	1489:1500	a proportion of total short-chain fatty acids in the feces	1489:1546	The SARA-inducing feeding challenge resulted in decreased fecal pH and increased butyrate as a proportion of total short-chain fatty acids in the feces.
33663865	4	127	theme	cows	807:810	arg1	total					769:773	A total	767:773	A total of 18 early-lactating Simmental cows	767:810	A total of 18 early-lactating Simmental cows were divided into 3 groups (n = 6); 2 were fed a SARA-inducing feeding regimen (60% concentrate), 1 was fed a control (CON) diet (40% concentrate).
33663865	2	128	theme	SARA	346:349	arg1	effect					336:341	the effect	332:341	the effect of SARA on hindgut microbiota, and whether this condition	332:399	Yet, the effect of SARA on hindgut microbiota, and whether this condition is aggravated by exogenous immune stimuli, is less understood.
35014302	0	0	theme	PEO	104:106	arg1	Nanofibers					53:62	Electroconductive Water-Resistant Nanofibers	19:62	Electroconductive Water-Resistant Nanofibers of PEDOT-PSS, Cellulose Nanofibrils and PEO	19:106	Electrospinning of Electroconductive Water-Resistant Nanofibers of PEDOT-PSS, Cellulose Nanofibrils and PEO: Fabrication, Characterization, and Cytocompatibility.
35014302	2	1	theme	diglycidyl	478:487	arg1	ether					489:493	poly(ethylene glycol) diglycidyl ether	456:493	poly(ethylene glycol) diglycidyl ether	456:493	Poly(ethylene oxide) (PEO) was used to assist the electrospinning process, and poly(ethylene glycol) diglycidyl ether was used to induce chemical cross-linking, enabling stability of the formed fibrous mats in water.
35014302	3	2	theme	bead-free	765:773	arg1	mats					787:790	bead-free nanofibrous mats	765:790	bead-free nanofibrous mats with high stability	765:810	The experimental parameters regarding the electrospinning polymer dispersion and electrospinning process were carefully studied to achieve a reproducible method to obtain bead-free nanofibrous mats with high stability after water contact, with an electrical conductivity of 13 ± 5 S m-1, thus making them suitable for bioelectrochemical applications.
35014302	3	3	theme	experimental	598:609	arg1	parameters					611:620	The experimental parameters	594:620	The experimental parameters regarding the electrospinning polymer dispersion and electrospinning process	594:697	The experimental parameters regarding the electrospinning polymer dispersion and electrospinning process were carefully studied to achieve a reproducible method to obtain bead-free nanofibrous mats with high stability after water contact, with an electrical conductivity of 13 ± 5 S m-1, thus making them suitable for bioelectrochemical applications.
35014302	1	4	theme	composite	187:195	arg1	nanofibers					197:206	Electrically conductive composite nanofibers	163:206	Electrically conductive composite nanofibers	163:206	Electrically conductive composite nanofibers were fabricated using poly(3,4-ethylenedioxythiophene) doped with poly(styrenesulfonate) (PEDOT-PSS) and cellulose nanofibrils (CNFs) via the electrospinning technique.
35014302	0	5	theme	Electroconductive	19:35	arg1	Nanofibers					53:62	Electroconductive Water-Resistant Nanofibers	19:62	Electroconductive Water-Resistant Nanofibers of PEDOT-PSS, Cellulose Nanofibrils and PEO	19:106	Electrospinning of Electroconductive Water-Resistant Nanofibers of PEDOT-PSS, Cellulose Nanofibrils and PEO: Fabrication, Characterization, and Cytocompatibility.
35014302	5	6	theme	PEDOT-PSS/CNF/PEO	1157:1173	arg1	fibers					1185:1190	the PEDOT-PSS/CNF/PEO composite fibers	1153:1190	the PEDOT-PSS/CNF/PEO composite fibers	1153:1190	Cyclic voltammetric studies showed that the PEDOT-PSS/CNF/PEO composite fibers exhibited high electroactivity and high stability in water for at least two months.
35014302	5	7	theme	composite	1175:1183	arg1	fibers					1185:1190	the PEDOT-PSS/CNF/PEO composite fibers	1153:1190	the PEDOT-PSS/CNF/PEO composite fibers	1153:1190	Cyclic voltammetric studies showed that the PEDOT-PSS/CNF/PEO composite fibers exhibited high electroactivity and high stability in water for at least two months.
35014302	3	8	theme	electrospinning	636:650	arg1	dispersion					660:669	the electrospinning polymer dispersion	632:669	the electrospinning polymer dispersion	632:669	The experimental parameters regarding the electrospinning polymer dispersion and electrospinning process were carefully studied to achieve a reproducible method to obtain bead-free nanofibrous mats with high stability after water contact, with an electrical conductivity of 13 ± 5 S m-1, thus making them suitable for bioelectrochemical applications.
35014302	3	9	theme	m-1	877:879	arg1	conductivity					852:863	an electrical conductivity	838:863	an electrical conductivity of 13 ± 5 S m-1	838:879	The experimental parameters regarding the electrospinning polymer dispersion and electrospinning process were carefully studied to achieve a reproducible method to obtain bead-free nanofibrous mats with high stability after water contact, with an electrical conductivity of 13 ± 5 S m-1, thus making them suitable for bioelectrochemical applications.
35014302	3	10	theme	polymer	652:658	arg1	dispersion					660:669	the electrospinning polymer dispersion	632:669	the electrospinning polymer dispersion	632:669	The experimental parameters regarding the electrospinning polymer dispersion and electrospinning process were carefully studied to achieve a reproducible method to obtain bead-free nanofibrous mats with high stability after water contact, with an electrical conductivity of 13 ± 5 S m-1, thus making them suitable for bioelectrochemical applications.
35014302	2	11	theme	chemical	514:521	arg1	cross-linking					523:535	chemical cross-linking	514:535	chemical cross-linking	514:535	Poly(ethylene oxide) (PEO) was used to assist the electrospinning process, and poly(ethylene glycol) diglycidyl ether was used to induce chemical cross-linking, enabling stability of the formed fibrous mats in water.
35014302	6	12	theme	fiber	1324:1328	arg1	due					1384:1386	due	1384:1386	due	1384:1386	By infrared spectroscopy, the slightly modified fiber morphology after water contact was demonstrated to be due to dissolution of some part of the PEO in the fiber structure.
35014302	6	12	theme	fiber	1324:1328	arg1	morphology					1330:1339	the slightly modified fiber morphology	1302:1339	the slightly modified fiber morphology after water contact	1302:1359	By infrared spectroscopy, the slightly modified fiber morphology after water contact was demonstrated to be due to dissolution of some part of the PEO in the fiber structure.
35014302	4	13	theme	energy	1079:1084	arg1	analysis					1103:1110	energy dispersive X-ray analysis	1079:1110	energy dispersive X-ray analysis	1079:1110	The morphology of the electrospun nanofibers was characterized by scanning electron microscopy, and the C/S ratio was determined with energy dispersive X-ray analysis.
35014302	7	14	theme	photosynthetic	1606:1619	arg1	cell					1640:1643	a photosynthetic bioelectrochemical cell	1604:1643	a photosynthetic bioelectrochemical cell	1604:1643	The biocompatibility of the PEDOT-PSS/CNF/PEO composite fibers when used as an electroconductive substrate to immobilize microalgae and cyanobacteria in a photosynthetic bioelectrochemical cell was also demonstrated.
35014302	5	15	theme	voltammetric	1120:1131	arg1	studies					1133:1139	Cyclic voltammetric studies	1113:1139	Cyclic voltammetric studies	1113:1139	Cyclic voltammetric studies showed that the PEDOT-PSS/CNF/PEO composite fibers exhibited high electroactivity and high stability in water for at least two months.
35014302	2	16	theme	mats	579:582	arg1	stability					547:555	stability	547:555	stability of the formed fibrous mats in water	547:591	Poly(ethylene oxide) (PEO) was used to assist the electrospinning process, and poly(ethylene glycol) diglycidyl ether was used to induce chemical cross-linking, enabling stability of the formed fibrous mats in water.
35014302	6	17	theme	modified	1315:1322	arg1	due					1384:1386	due	1384:1386	due	1384:1386	By infrared spectroscopy, the slightly modified fiber morphology after water contact was demonstrated to be due to dissolution of some part of the PEO in the fiber structure.
35014302	6	17	theme	modified	1315:1322	arg1	morphology					1330:1339	the slightly modified fiber morphology	1302:1339	the slightly modified fiber morphology after water contact	1302:1359	By infrared spectroscopy, the slightly modified fiber morphology after water contact was demonstrated to be due to dissolution of some part of the PEO in the fiber structure.
35014302	2	18	theme	ethylene	382:389	arg1	Poly					377:380	Poly	377:380	Poly(ethylene oxide) (PEO)	377:402	Poly(ethylene oxide) (PEO) was used to assist the electrospinning process, and poly(ethylene glycol) diglycidyl ether was used to induce chemical cross-linking, enabling stability of the formed fibrous mats in water.
35014302	2	18	theme	ethylene	382:389	arg1	oxide					391:395	ethylene oxide	382:395	ethylene oxide	382:395	Poly(ethylene oxide) (PEO) was used to assist the electrospinning process, and poly(ethylene glycol) diglycidyl ether was used to induce chemical cross-linking, enabling stability of the formed fibrous mats in water.
35014302	4	19	theme	electron	1020:1027	arg1	microscopy					1029:1038	scanning electron microscopy	1011:1038	scanning electron microscopy	1011:1038	The morphology of the electrospun nanofibers was characterized by scanning electron microscopy, and the C/S ratio was determined with energy dispersive X-ray analysis.
35014302	3	20	theme	electrical	841:850	arg1	conductivity					852:863	an electrical conductivity	838:863	an electrical conductivity of 13 ± 5 S m-1	838:879	The experimental parameters regarding the electrospinning polymer dispersion and electrospinning process were carefully studied to achieve a reproducible method to obtain bead-free nanofibrous mats with high stability after water contact, with an electrical conductivity of 13 ± 5 S m-1, thus making them suitable for bioelectrochemical applications.
35014302	4	21	theme	nanofibers	979:988	arg1	morphology					949:958	The morphology	945:958	The morphology of the electrospun nanofibers	945:988	The morphology of the electrospun nanofibers was characterized by scanning electron microscopy, and the C/S ratio was determined with energy dispersive X-ray analysis.
35014302	6	22	from	dissolution	1391:1401	arg1	structure					1440:1448	the fiber structure	1430:1448	the fiber structure	1430:1448	By infrared spectroscopy, the slightly modified fiber morphology after water contact was demonstrated to be due to dissolution of some part of the PEO in the fiber structure.
35014302	3	23	dep	S	875:875	arg1	±					871:871	±	871:871	±	871:871	The experimental parameters regarding the electrospinning polymer dispersion and electrospinning process were carefully studied to achieve a reproducible method to obtain bead-free nanofibrous mats with high stability after water contact, with an electrical conductivity of 13 ± 5 S m-1, thus making them suitable for bioelectrochemical applications.
35014302	3	24	theme	bioelectrochemical	912:929	arg1	applications					931:942	bioelectrochemical applications	912:942	bioelectrochemical applications	912:942	The experimental parameters regarding the electrospinning polymer dispersion and electrospinning process were carefully studied to achieve a reproducible method to obtain bead-free nanofibrous mats with high stability after water contact, with an electrical conductivity of 13 ± 5 S m-1, thus making them suitable for bioelectrochemical applications.
35014302	5	25	theme	Cyclic	1113:1118	arg1	studies					1133:1139	Cyclic voltammetric studies	1113:1139	Cyclic voltammetric studies	1113:1139	Cyclic voltammetric studies showed that the PEDOT-PSS/CNF/PEO composite fibers exhibited high electroactivity and high stability in water for at least two months.
35014302	2	26	theme	poly	456:459	arg1	ether					489:493	poly(ethylene glycol) diglycidyl ether	456:493	poly(ethylene glycol) diglycidyl ether	456:493	Poly(ethylene oxide) (PEO) was used to assist the electrospinning process, and poly(ethylene glycol) diglycidyl ether was used to induce chemical cross-linking, enabling stability of the formed fibrous mats in water.
35014302	7	27	theme	PEDOT-PSS/CNF/PEO	1479:1495	arg1	fibers					1507:1512	the PEDOT-PSS/CNF/PEO composite fibers	1475:1512	the PEDOT-PSS/CNF/PEO composite fibers	1475:1512	The biocompatibility of the PEDOT-PSS/CNF/PEO composite fibers when used as an electroconductive substrate to immobilize microalgae and cyanobacteria in a photosynthetic bioelectrochemical cell was also demonstrated.
35014302	3	28	dep	m-1	877:879	arg1	S					875:875	S	875:875	S	875:875	The experimental parameters regarding the electrospinning polymer dispersion and electrospinning process were carefully studied to achieve a reproducible method to obtain bead-free nanofibrous mats with high stability after water contact, with an electrical conductivity of 13 ± 5 S m-1, thus making them suitable for bioelectrochemical applications.
35014302	7	29	theme	composite	1497:1505	arg1	fibers					1507:1512	the PEDOT-PSS/CNF/PEO composite fibers	1475:1512	the PEDOT-PSS/CNF/PEO composite fibers	1475:1512	The biocompatibility of the PEDOT-PSS/CNF/PEO composite fibers when used as an electroconductive substrate to immobilize microalgae and cyanobacteria in a photosynthetic bioelectrochemical cell was also demonstrated.
35014302	4	30	theme	C/S	1049:1051	arg1	ratio					1053:1057	the C/S ratio	1045:1057	the C/S ratio	1045:1057	The morphology of the electrospun nanofibers was characterized by scanning electron microscopy, and the C/S ratio was determined with energy dispersive X-ray analysis.
35014302	3	31	theme	reproducible	735:746	arg1	method					748:753	a reproducible method	733:753	a reproducible method to obtain bead-free nanofibrous mats with high stability after water contact, with an electrical conductivity of 13 ± 5 S m-1, thus making them suitable for bioelectrochemical applications	733:942	The experimental parameters regarding the electrospinning polymer dispersion and electrospinning process were carefully studied to achieve a reproducible method to obtain bead-free nanofibrous mats with high stability after water contact, with an electrical conductivity of 13 ± 5 S m-1, thus making them suitable for bioelectrochemical applications.
35014302	5	32	theme	high	1227:1230	arg1	stability					1232:1240	high stability	1227:1240	high stability	1227:1240	Cyclic voltammetric studies showed that the PEDOT-PSS/CNF/PEO composite fibers exhibited high electroactivity and high stability in water for at least two months.
35014302	3	33	theme	nanofibrous	775:785	arg1	mats					787:790	bead-free nanofibrous mats	765:790	bead-free nanofibrous mats with high stability	765:810	The experimental parameters regarding the electrospinning polymer dispersion and electrospinning process were carefully studied to achieve a reproducible method to obtain bead-free nanofibrous mats with high stability after water contact, with an electrical conductivity of 13 ± 5 S m-1, thus making them suitable for bioelectrochemical applications.
35014302	7	34	theme	electroconductive	1530:1546	arg1	substrate					1548:1556	an electroconductive substrate	1527:1556	an electroconductive substrate to immobilize microalgae and cyanobacteria in a photosynthetic bioelectrochemical cell	1527:1643	The biocompatibility of the PEDOT-PSS/CNF/PEO composite fibers when used as an electroconductive substrate to immobilize microalgae and cyanobacteria in a photosynthetic bioelectrochemical cell was also demonstrated.
35014302	0	35	theme	Water-Resistant	37:51	arg1	Nanofibers					53:62	Electroconductive Water-Resistant Nanofibers	19:62	Electroconductive Water-Resistant Nanofibers of PEDOT-PSS, Cellulose Nanofibrils and PEO	19:106	Electrospinning of Electroconductive Water-Resistant Nanofibers of PEDOT-PSS, Cellulose Nanofibrils and PEO: Fabrication, Characterization, and Cytocompatibility.
35014302	1	36	theme	cellulose	313:321	arg1	nanofibrils					323:333	cellulose nanofibrils	313:333	cellulose nanofibrils (CNFs)	313:340	Electrically conductive composite nanofibers were fabricated using poly(3,4-ethylenedioxythiophene) doped with poly(styrenesulfonate) (PEDOT-PSS) and cellulose nanofibrils (CNFs) via the electrospinning technique.
35014302	1	36	theme	cellulose	313:321	arg1	CNFs					336:339	CNFs	336:339	CNFs	336:339	Electrically conductive composite nanofibers were fabricated using poly(3,4-ethylenedioxythiophene) doped with poly(styrenesulfonate) (PEDOT-PSS) and cellulose nanofibrils (CNFs) via the electrospinning technique.
35014302	3	37	with	mats	787:790	arg1	stability					802:810	high stability	797:810	high stability	797:810	The experimental parameters regarding the electrospinning polymer dispersion and electrospinning process were carefully studied to achieve a reproducible method to obtain bead-free nanofibrous mats with high stability after water contact, with an electrical conductivity of 13 ± 5 S m-1, thus making them suitable for bioelectrochemical applications.
35014302	4	38	theme	scanning	1011:1018	arg1	microscopy					1029:1038	scanning electron microscopy	1011:1038	scanning electron microscopy	1011:1038	The morphology of the electrospun nanofibers was characterized by scanning electron microscopy, and the C/S ratio was determined with energy dispersive X-ray analysis.
35014302	2	39	theme	electrospinning	427:441	arg1	process					443:449	the electrospinning process	423:449	the electrospinning process	423:449	Poly(ethylene oxide) (PEO) was used to assist the electrospinning process, and poly(ethylene glycol) diglycidyl ether was used to induce chemical cross-linking, enabling stability of the formed fibrous mats in water.
35014302	2	40	used	used	499:502	arg2	ether					489:493	poly(ethylene glycol) diglycidyl ether	456:493	poly(ethylene glycol) diglycidyl ether	456:493	Poly(ethylene oxide) (PEO) was used to assist the electrospinning process, and poly(ethylene glycol) diglycidyl ether was used to induce chemical cross-linking, enabling stability of the formed fibrous mats in water.
35014302	2	41	theme	glycol	470:475	arg1	ether					489:493	poly(ethylene glycol) diglycidyl ether	456:493	poly(ethylene glycol) diglycidyl ether	456:493	Poly(ethylene oxide) (PEO) was used to assist the electrospinning process, and poly(ethylene glycol) diglycidyl ether was used to induce chemical cross-linking, enabling stability of the formed fibrous mats in water.
35014302	2	42	from	water	587:591	arg1	stability					547:555	stability	547:555	stability of the formed fibrous mats in water	547:591	Poly(ethylene oxide) (PEO) was used to assist the electrospinning process, and poly(ethylene glycol) diglycidyl ether was used to induce chemical cross-linking, enabling stability of the formed fibrous mats in water.
35014302	4	43	theme	dispersive	1086:1095	arg1	analysis					1103:1110	energy dispersive X-ray analysis	1079:1110	energy dispersive X-ray analysis	1079:1110	The morphology of the electrospun nanofibers was characterized by scanning electron microscopy, and the C/S ratio was determined with energy dispersive X-ray analysis.
35014302	6	44	theme	infrared	1279:1286	arg1	spectroscopy					1288:1299	infrared spectroscopy	1279:1299	infrared spectroscopy	1279:1299	By infrared spectroscopy, the slightly modified fiber morphology after water contact was demonstrated to be due to dissolution of some part of the PEO in the fiber structure.
35014302	0	45	theme	Nanofibers	53:62	arg1	Electrospinning					0:14	Electrospinning	0:14	Electrospinning of Electroconductive Water-Resistant Nanofibers of PEDOT-PSS, Cellulose Nanofibrils and PEO: Fabrication, Characterization, and Cytocompatibility.	0:161	Electrospinning of Electroconductive Water-Resistant Nanofibers of PEDOT-PSS, Cellulose Nanofibrils and PEO: Fabrication, Characterization, and Cytocompatibility.
35014302	6	46	theme	PEO	1423:1425	arg1	part					1411:1414	some part	1406:1414	some part of the PEO	1406:1425	By infrared spectroscopy, the slightly modified fiber morphology after water contact was demonstrated to be due to dissolution of some part of the PEO in the fiber structure.
35014302	2	47	theme	ethylene	461:468	arg1	ether					489:493	poly(ethylene glycol) diglycidyl ether	456:493	poly(ethylene glycol) diglycidyl ether	456:493	Poly(ethylene oxide) (PEO) was used to assist the electrospinning process, and poly(ethylene glycol) diglycidyl ether was used to induce chemical cross-linking, enabling stability of the formed fibrous mats in water.
35014302	3	48	theme	water	818:822	arg1	contact					824:830	water contact	818:830	water contact	818:830	The experimental parameters regarding the electrospinning polymer dispersion and electrospinning process were carefully studied to achieve a reproducible method to obtain bead-free nanofibrous mats with high stability after water contact, with an electrical conductivity of 13 ± 5 S m-1, thus making them suitable for bioelectrochemical applications.
35014302	2	49	used	used	408:411	arg2	oxide					391:395	ethylene oxide	382:395	ethylene oxide	382:395	Poly(ethylene oxide) (PEO) was used to assist the electrospinning process, and poly(ethylene glycol) diglycidyl ether was used to induce chemical cross-linking, enabling stability of the formed fibrous mats in water.
35014302	2	49	used	used	408:411	arg2	Poly					377:380	Poly	377:380	Poly(ethylene oxide) (PEO)	377:402	Poly(ethylene oxide) (PEO) was used to assist the electrospinning process, and poly(ethylene glycol) diglycidyl ether was used to induce chemical cross-linking, enabling stability of the formed fibrous mats in water.
35014302	2	49	used	used	408:411	arg2	PEO					399:401	PEO	399:401	PEO	399:401	Poly(ethylene oxide) (PEO) was used to assist the electrospinning process, and poly(ethylene glycol) diglycidyl ether was used to induce chemical cross-linking, enabling stability of the formed fibrous mats in water.
35014302	4	50	theme	electrospun	967:977	arg1	nanofibers					979:988	the electrospun nanofibers	963:988	the electrospun nanofibers	963:988	The morphology of the electrospun nanofibers was characterized by scanning electron microscopy, and the C/S ratio was determined with energy dispersive X-ray analysis.
35014302	6	51	theme	fiber	1434:1438	arg1	structure					1440:1448	the fiber structure	1430:1448	the fiber structure	1430:1448	By infrared spectroscopy, the slightly modified fiber morphology after water contact was demonstrated to be due to dissolution of some part of the PEO in the fiber structure.
35014302	5	52	theme	high	1202:1205	arg1	electroactivity					1207:1221	high electroactivity	1202:1221	high electroactivity	1202:1221	Cyclic voltammetric studies showed that the PEDOT-PSS/CNF/PEO composite fibers exhibited high electroactivity and high stability in water for at least two months.
35014302	2	53	theme	fibrous	571:577	arg1	mats					579:582	the formed fibrous mats	560:582	the formed fibrous mats in water	560:591	Poly(ethylene oxide) (PEO) was used to assist the electrospinning process, and poly(ethylene glycol) diglycidyl ether was used to induce chemical cross-linking, enabling stability of the formed fibrous mats in water.
35014302	2	54	from	mats	579:582	arg1	water					587:591	water	587:591	water	587:591	Poly(ethylene oxide) (PEO) was used to assist the electrospinning process, and poly(ethylene glycol) diglycidyl ether was used to induce chemical cross-linking, enabling stability of the formed fibrous mats in water.
35014302	6	55	theme	part	1411:1414	arg1	dissolution					1391:1401	dissolution	1391:1401	dissolution of some part of the PEO in the fiber structure	1391:1448	By infrared spectroscopy, the slightly modified fiber morphology after water contact was demonstrated to be due to dissolution of some part of the PEO in the fiber structure.
35014302	0	56	theme	PEDOT-PSS	67:75	arg1	Nanofibers					53:62	Electroconductive Water-Resistant Nanofibers	19:62	Electroconductive Water-Resistant Nanofibers of PEDOT-PSS, Cellulose Nanofibrils and PEO	19:106	Electrospinning of Electroconductive Water-Resistant Nanofibers of PEDOT-PSS, Cellulose Nanofibrils and PEO: Fabrication, Characterization, and Cytocompatibility.
35014302	2	57	theme	formed	564:569	arg1	mats					579:582	the formed fibrous mats	560:582	the formed fibrous mats in water	560:591	Poly(ethylene oxide) (PEO) was used to assist the electrospinning process, and poly(ethylene glycol) diglycidyl ether was used to induce chemical cross-linking, enabling stability of the formed fibrous mats in water.
35014302	3	58	theme	5	873:873	arg1	±					871:871	±	871:871	±	871:871	The experimental parameters regarding the electrospinning polymer dispersion and electrospinning process were carefully studied to achieve a reproducible method to obtain bead-free nanofibrous mats with high stability after water contact, with an electrical conductivity of 13 ± 5 S m-1, thus making them suitable for bioelectrochemical applications.
35014302	3	59	theme	high	797:800	arg1	stability					802:810	high stability	797:810	high stability	797:810	The experimental parameters regarding the electrospinning polymer dispersion and electrospinning process were carefully studied to achieve a reproducible method to obtain bead-free nanofibrous mats with high stability after water contact, with an electrical conductivity of 13 ± 5 S m-1, thus making them suitable for bioelectrochemical applications.
35014302	4	60	theme	X-ray	1097:1101	arg1	analysis					1103:1110	energy dispersive X-ray analysis	1079:1110	energy dispersive X-ray analysis	1079:1110	The morphology of the electrospun nanofibers was characterized by scanning electron microscopy, and the C/S ratio was determined with energy dispersive X-ray analysis.
35014302	6	61	theme	water	1347:1351	arg1	contact					1353:1359	water contact	1347:1359	water contact	1347:1359	By infrared spectroscopy, the slightly modified fiber morphology after water contact was demonstrated to be due to dissolution of some part of the PEO in the fiber structure.
35014302	7	62	theme	fibers	1507:1512	arg1	biocompatibility					1455:1470	The biocompatibility	1451:1470	The biocompatibility of the PEDOT-PSS/CNF/PEO composite fibers	1451:1512	The biocompatibility of the PEDOT-PSS/CNF/PEO composite fibers when used as an electroconductive substrate to immobilize microalgae and cyanobacteria in a photosynthetic bioelectrochemical cell was also demonstrated.
35014302	3	63	theme	13	868:869	arg1	±					871:871	±	871:871	±	871:871	The experimental parameters regarding the electrospinning polymer dispersion and electrospinning process were carefully studied to achieve a reproducible method to obtain bead-free nanofibrous mats with high stability after water contact, with an electrical conductivity of 13 ± 5 S m-1, thus making them suitable for bioelectrochemical applications.
35014302	0	64	theme	Nanofibrils	88:98	arg1	Nanofibers					53:62	Electroconductive Water-Resistant Nanofibers	19:62	Electroconductive Water-Resistant Nanofibers of PEDOT-PSS, Cellulose Nanofibrils and PEO	19:106	Electrospinning of Electroconductive Water-Resistant Nanofibers of PEDOT-PSS, Cellulose Nanofibrils and PEO: Fabrication, Characterization, and Cytocompatibility.
35014302	3	65	theme	electrospinning	675:689	arg1	process					691:697	electrospinning process	675:697	electrospinning process	675:697	The experimental parameters regarding the electrospinning polymer dispersion and electrospinning process were carefully studied to achieve a reproducible method to obtain bead-free nanofibrous mats with high stability after water contact, with an electrical conductivity of 13 ± 5 S m-1, thus making them suitable for bioelectrochemical applications.
35014302	7	66	theme	bioelectrochemical	1621:1638	arg1	cell					1640:1643	a photosynthetic bioelectrochemical cell	1604:1643	a photosynthetic bioelectrochemical cell	1604:1643	The biocompatibility of the PEDOT-PSS/CNF/PEO composite fibers when used as an electroconductive substrate to immobilize microalgae and cyanobacteria in a photosynthetic bioelectrochemical cell was also demonstrated.
35014302	3	67	with	contact	824:830	arg1	conductivity					852:863	an electrical conductivity	838:863	an electrical conductivity of 13 ± 5 S m-1	838:879	The experimental parameters regarding the electrospinning polymer dispersion and electrospinning process were carefully studied to achieve a reproducible method to obtain bead-free nanofibrous mats with high stability after water contact, with an electrical conductivity of 13 ± 5 S m-1, thus making them suitable for bioelectrochemical applications.
35014302	2	68	from	stability	547:555	arg1	water					587:591	water	587:591	water	587:591	Poly(ethylene oxide) (PEO) was used to assist the electrospinning process, and poly(ethylene glycol) diglycidyl ether was used to induce chemical cross-linking, enabling stability of the formed fibrous mats in water.
35014302	0	69	theme	Cellulose	78:86	arg1	Nanofibrils					88:98	Cellulose Nanofibrils	78:98	Cellulose Nanofibrils	78:98	Electrospinning of Electroconductive Water-Resistant Nanofibers of PEDOT-PSS, Cellulose Nanofibrils and PEO: Fabrication, Characterization, and Cytocompatibility.
35014302	1	70	theme	electrospinning	350:364	arg1	technique					366:374	the electrospinning technique	346:374	the electrospinning technique	346:374	Electrically conductive composite nanofibers were fabricated using poly(3,4-ethylenedioxythiophene) doped with poly(styrenesulfonate) (PEDOT-PSS) and cellulose nanofibrils (CNFs) via the electrospinning technique.
35014302	0	71	dep	Electrospinning	0:14	arg1	Cytocompatibility					144:160	Cytocompatibility	144:160	Cytocompatibility	144:160	Electrospinning of Electroconductive Water-Resistant Nanofibers of PEDOT-PSS, Cellulose Nanofibrils and PEO: Fabrication, Characterization, and Cytocompatibility.
35014302	0	71	dep	Electrospinning	0:14	arg1	Fabrication					109:119	Fabrication	109:119	Fabrication	109:119	Electrospinning of Electroconductive Water-Resistant Nanofibers of PEDOT-PSS, Cellulose Nanofibrils and PEO: Fabrication, Characterization, and Cytocompatibility.
35014302	0	71	dep	Electrospinning	0:14	arg1	Characterization					122:137	Characterization	122:137	Characterization	122:137	Electrospinning of Electroconductive Water-Resistant Nanofibers of PEDOT-PSS, Cellulose Nanofibrils and PEO: Fabrication, Characterization, and Cytocompatibility.
35014302	1	72	theme	conductive	176:185	arg1	nanofibers					197:206	Electrically conductive composite nanofibers	163:206	Electrically conductive composite nanofibers	163:206	Electrically conductive composite nanofibers were fabricated using poly(3,4-ethylenedioxythiophene) doped with poly(styrenesulfonate) (PEDOT-PSS) and cellulose nanofibrils (CNFs) via the electrospinning technique.
34044939	6	0	theme	mechanical	1095:1104	arg1	features					1106:1113	the enhanced mechanical features	1082:1113	the enhanced mechanical features	1082:1113	Overall, according to the enhanced mechanical features, suitable environment for cellular growth, and sustained drug release, the optimized scaffold would be a good candidate for tissue regeneration.
34044939	1	1	theme	Gelatin	259:265	arg1	mat					279:281	Gelatin electrospun mat	259:281	PCL:Gelatin electrospun mat	255:281	In this study, we fabricated two different arrangements of laminated composite scaffolds based on Alginate:Alginate sulfate hydrogel, PCL:Gelatin electrospun mat, and Kartogenin-PLGA nanoparticles (KGN-NPs).
34044939	0	2	theme	tissue	101:106	arg1	engineering					108:118	tissue engineering	101:118	tissue engineering	101:118	Alginate sulfate-based hydrogel/nanofiber composite scaffold with controlled Kartogenin delivery for tissue engineering.
34044939	2	3	theme	controlled	590:599	arg1	biodegradability					601:616	controlled biodegradability	590:616	controlled biodegradability	590:616	The optimized composite scaffold revealed a range of advantages such as improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability.
34044939	1	4	theme	laminated	180:188	arg1	scaffolds					200:208	laminated composite scaffolds	180:208	laminated composite scaffolds based on Alginate	180:226	In this study, we fabricated two different arrangements of laminated composite scaffolds based on Alginate:Alginate sulfate hydrogel, PCL:Gelatin electrospun mat, and Kartogenin-PLGA nanoparticles (KGN-NPs).
34044939	5	5	contain	has	1005:1007	arg1	scaffold					996:1003	the optimized composite scaffold	972:1003	the optimized composite scaffold	972:1003	Resazurin assay and Live/Dead staining revealed that the optimized composite scaffold has no cytotoxic effect and could improve cell growth.
34044939	5	5	contain	has	1005:1007	arg2	effect					1022:1027	no cytotoxic effect	1009:1027	no cytotoxic effect	1009:1027	Resazurin assay and Live/Dead staining revealed that the optimized composite scaffold has no cytotoxic effect and could improve cell growth.
34044939	1	6	theme	electrospun	267:277	arg1	mat					279:281	Gelatin electrospun mat	259:281	PCL:Gelatin electrospun mat	255:281	In this study, we fabricated two different arrangements of laminated composite scaffolds based on Alginate:Alginate sulfate hydrogel, PCL:Gelatin electrospun mat, and Kartogenin-PLGA nanoparticles (KGN-NPs).
34044939	2	7	theme	fiber	529:533	arg1	pores					507:511	well as less potential of damage (less dissipated energy), interconnected pores	433:511	well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability	433:616	The optimized composite scaffold revealed a range of advantages such as improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability.
34044939	3	8	theme	composite	709:717	arg1	scaffold					719:726	the composite scaffold	705:726	the composite scaffold	705:726	Furthermore, the synthesized KGN-NPs with spherical morphology were incorporated into the composite scaffold and exhibited a linear and sustained release of KGN within 30 days with desirable initial burst reduction (12% vs. 20%).
34044939	3	9	with	30 days	787:793	arg1	reduction					824:832	desirable initial burst reduction	800:832	desirable initial burst reduction (12% vs. 20%)	800:846	Furthermore, the synthesized KGN-NPs with spherical morphology were incorporated into the composite scaffold and exhibited a linear and sustained release of KGN within 30 days with desirable initial burst reduction (12% vs. 20%).
34044939	3	10	theme	linear	744:749	arg1	release					765:771	a linear and sustained release	742:771	a linear and sustained release of KGN	742:778	Furthermore, the synthesized KGN-NPs with spherical morphology were incorporated into the composite scaffold and exhibited a linear and sustained release of KGN within 30 days with desirable initial burst reduction (12% vs. 20%).
34044939	4	11	theme	cytotoxicity	867:878	arg1	impact					880:885	the cytotoxicity impact	863:885	the cytotoxicity impact of the composite	863:902	Additionally, the cytotoxicity impact of the composite was evaluated.
34044939	6	12	theme	suitable	1116:1123	arg1	environment					1125:1135	suitable environment	1116:1135	suitable environment for cellular growth	1116:1155	Overall, according to the enhanced mechanical features, suitable environment for cellular growth, and sustained drug release, the optimized scaffold would be a good candidate for tissue regeneration.
34044939	2	13	theme	hydrogel	516:523	arg1	pores					507:511	well as less potential of damage (less dissipated energy), interconnected pores	433:511	well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability	433:616	The optimized composite scaffold revealed a range of advantages such as improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability.
34044939	2	14	dep	potential	446:454	arg1	interconnected					492:505	interconnected	492:505	interconnected	492:505	The optimized composite scaffold revealed a range of advantages such as improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability.
34044939	5	15	theme	optimized	976:984	arg1	scaffold					996:1003	the optimized composite scaffold	972:1003	the optimized composite scaffold	972:1003	Resazurin assay and Live/Dead staining revealed that the optimized composite scaffold has no cytotoxic effect and could improve cell growth.
34044939	1	16	theme	composite	190:198	arg1	scaffolds					200:208	laminated composite scaffolds	180:208	laminated composite scaffolds based on Alginate	180:226	In this study, we fabricated two different arrangements of laminated composite scaffolds based on Alginate:Alginate sulfate hydrogel, PCL:Gelatin electrospun mat, and Kartogenin-PLGA nanoparticles (KGN-NPs).
34044939	2	17	with	hydrogel	516:523	arg1	biodegradability					601:616	controlled biodegradability	590:616	controlled biodegradability	590:616	The optimized composite scaffold revealed a range of advantages such as improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability.
34044939	2	17	with	hydrogel	516:523	arg1	size					554:557	adequate pore size	540:557	adequate pore size	540:557	The optimized composite scaffold revealed a range of advantages such as improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability.
34044939	2	17	with	hydrogel	516:523	arg1	ratio					579:583	excellent swelling ratio	560:583	excellent swelling ratio	560:583	The optimized composite scaffold revealed a range of advantages such as improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability.
34044939	5	18	theme	composite	986:994	arg1	scaffold					996:1003	the optimized composite scaffold	972:1003	the optimized composite scaffold	972:1003	Resazurin assay and Live/Dead staining revealed that the optimized composite scaffold has no cytotoxic effect and could improve cell growth.
34044939	1	19	theme	scaffolds	200:208	arg1	arrangements					164:175	two different arrangements	150:175	two different arrangements of laminated composite scaffolds based on Alginate	150:226	In this study, we fabricated two different arrangements of laminated composite scaffolds based on Alginate:Alginate sulfate hydrogel, PCL:Gelatin electrospun mat, and Kartogenin-PLGA nanoparticles (KGN-NPs).
34044939	2	20	theme	damage	459:464	arg1	potential					446:454	potential	446:454	potential	446:454	The optimized composite scaffold revealed a range of advantages such as improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability.
34044939	6	21	theme	sustained	1162:1170	arg1	release					1177:1183	sustained drug release	1162:1183	sustained drug release	1162:1183	Overall, according to the enhanced mechanical features, suitable environment for cellular growth, and sustained drug release, the optimized scaffold would be a good candidate for tissue regeneration.
34044939	6	22	theme	tissue	1239:1244	arg1	regeneration					1246:1257	tissue regeneration	1239:1257	tissue regeneration	1239:1257	Overall, according to the enhanced mechanical features, suitable environment for cellular growth, and sustained drug release, the optimized scaffold would be a good candidate for tissue regeneration.
34044939	2	23	with	fiber	529:533	arg1	biodegradability					601:616	controlled biodegradability	590:616	controlled biodegradability	590:616	The optimized composite scaffold revealed a range of advantages such as improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability.
34044939	2	23	with	fiber	529:533	arg1	size					554:557	adequate pore size	540:557	adequate pore size	540:557	The optimized composite scaffold revealed a range of advantages such as improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability.
34044939	2	23	with	fiber	529:533	arg1	ratio					579:583	excellent swelling ratio	560:583	excellent swelling ratio	560:583	The optimized composite scaffold revealed a range of advantages such as improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability.
34044939	5	24	theme	Live/Dead	939:947	arg1	staining					949:956	Live/Dead staining	939:956	Live/Dead staining	939:956	Resazurin assay and Live/Dead staining revealed that the optimized composite scaffold has no cytotoxic effect and could improve cell growth.
34044939	0	25	theme	composite	42:50	arg1	scaffold					52:59	hydrogel/nanofiber composite scaffold	23:59	hydrogel/nanofiber composite scaffold	23:59	Alginate sulfate-based hydrogel/nanofiber composite scaffold with controlled Kartogenin delivery for tissue engineering.
34044939	1	26	theme	Kartogenin-PLGA	288:302	arg1	nanoparticles					304:316	Kartogenin-PLGA nanoparticles	288:316	Kartogenin-PLGA nanoparticles (KGN-NPs)	288:326	In this study, we fabricated two different arrangements of laminated composite scaffolds based on Alginate:Alginate sulfate hydrogel, PCL:Gelatin electrospun mat, and Kartogenin-PLGA nanoparticles (KGN-NPs).
34044939	1	26	theme	Kartogenin-PLGA	288:302	arg1	KGN-NPs					319:325	KGN-NPs	319:325	KGN-NPs	319:325	In this study, we fabricated two different arrangements of laminated composite scaffolds based on Alginate:Alginate sulfate hydrogel, PCL:Gelatin electrospun mat, and Kartogenin-PLGA nanoparticles (KGN-NPs).
34044939	3	27	with	KGN-NPs	648:654	arg1	morphology					671:680	spherical morphology	661:680	spherical morphology	661:680	Furthermore, the synthesized KGN-NPs with spherical morphology were incorporated into the composite scaffold and exhibited a linear and sustained release of KGN within 30 days with desirable initial burst reduction (12% vs. 20%).
34044939	0	28	theme	hydrogel/nanofiber	23:40	arg1	scaffold					52:59	hydrogel/nanofiber composite scaffold	23:59	hydrogel/nanofiber composite scaffold	23:59	Alginate sulfate-based hydrogel/nanofiber composite scaffold with controlled Kartogenin delivery for tissue engineering.
34044939	3	29	theme	sustained	755:763	arg1	release					765:771	a linear and sustained release	742:771	a linear and sustained release of KGN	742:778	Furthermore, the synthesized KGN-NPs with spherical morphology were incorporated into the composite scaffold and exhibited a linear and sustained release of KGN within 30 days with desirable initial burst reduction (12% vs. 20%).
34044939	2	30	theme	pore	549:552	arg1	size					554:557	adequate pore size	540:557	adequate pore size	540:557	The optimized composite scaffold revealed a range of advantages such as improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability.
34044939	1	31	dep	PCL	255:257	arg1	mat					279:281	Gelatin electrospun mat	259:281	PCL:Gelatin electrospun mat	255:281	In this study, we fabricated two different arrangements of laminated composite scaffolds based on Alginate:Alginate sulfate hydrogel, PCL:Gelatin electrospun mat, and Kartogenin-PLGA nanoparticles (KGN-NPs).
34044939	6	32	theme	enhanced	1086:1093	arg1	features					1106:1113	the enhanced mechanical features	1082:1113	the enhanced mechanical features	1082:1113	Overall, according to the enhanced mechanical features, suitable environment for cellular growth, and sustained drug release, the optimized scaffold would be a good candidate for tissue regeneration.
34044939	1	33	theme	different	154:162	arg1	arrangements					164:175	two different arrangements	150:175	two different arrangements of laminated composite scaffolds based on Alginate	150:226	In this study, we fabricated two different arrangements of laminated composite scaffolds based on Alginate:Alginate sulfate hydrogel, PCL:Gelatin electrospun mat, and Kartogenin-PLGA nanoparticles (KGN-NPs).
34044939	2	34	theme	adequate	540:547	arg1	size					554:557	adequate pore size	540:557	adequate pore size	540:557	The optimized composite scaffold revealed a range of advantages such as improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability.
34044939	3	35	theme	spherical	661:669	arg1	morphology					671:680	spherical morphology	661:680	spherical morphology	661:680	Furthermore, the synthesized KGN-NPs with spherical morphology were incorporated into the composite scaffold and exhibited a linear and sustained release of KGN within 30 days with desirable initial burst reduction (12% vs. 20%).
34044939	1	36	theme	Alginate	228:235	arg1	hydrogel					245:252	Alginate sulfate hydrogel	228:252	Alginate sulfate hydrogel	228:252	In this study, we fabricated two different arrangements of laminated composite scaffolds based on Alginate:Alginate sulfate hydrogel, PCL:Gelatin electrospun mat, and Kartogenin-PLGA nanoparticles (KGN-NPs).
34044939	2	37	theme	mechanical	410:419	arg1	features					421:428	improved mechanical features	401:428	improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability	401:616	The optimized composite scaffold revealed a range of advantages such as improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability.
34044939	2	38	theme	dissipated	472:481	arg1	damage					459:464	damage	459:464	damage (less dissipated energy)	459:489	The optimized composite scaffold revealed a range of advantages such as improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability.
34044939	2	38	theme	dissipated	472:481	arg1	energy					483:488	less dissipated energy	467:488	less dissipated energy	467:488	The optimized composite scaffold revealed a range of advantages such as improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability.
34044939	3	39	theme	synthesized	636:646	arg1	KGN-NPs					648:654	the synthesized KGN-NPs	632:654	the synthesized KGN-NPs with spherical morphology	632:680	Furthermore, the synthesized KGN-NPs with spherical morphology were incorporated into the composite scaffold and exhibited a linear and sustained release of KGN within 30 days with desirable initial burst reduction (12% vs. 20%).
34044939	2	40	theme	potential	446:454	arg1	pores					507:511	well as less potential of damage (less dissipated energy), interconnected pores	433:511	well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability	433:616	The optimized composite scaffold revealed a range of advantages such as improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability.
34044939	1	41	theme	sulfate	237:243	arg1	hydrogel					245:252	Alginate sulfate hydrogel	228:252	Alginate sulfate hydrogel	228:252	In this study, we fabricated two different arrangements of laminated composite scaffolds based on Alginate:Alginate sulfate hydrogel, PCL:Gelatin electrospun mat, and Kartogenin-PLGA nanoparticles (KGN-NPs).
34044939	0	42	theme	Kartogenin	77:86	arg1	delivery					88:95	controlled Kartogenin delivery	66:95	controlled Kartogenin delivery for tissue engineering	66:118	Alginate sulfate-based hydrogel/nanofiber composite scaffold with controlled Kartogenin delivery for tissue engineering.
34044939	3	43	theme	desirable	800:808	arg1	reduction					824:832	desirable initial burst reduction	800:832	desirable initial burst reduction (12% vs. 20%)	800:846	Furthermore, the synthesized KGN-NPs with spherical morphology were incorporated into the composite scaffold and exhibited a linear and sustained release of KGN within 30 days with desirable initial burst reduction (12% vs. 20%).
34044939	3	44	theme	KGN	776:778	arg1	release					765:771	a linear and sustained release	742:771	a linear and sustained release of KGN	742:778	Furthermore, the synthesized KGN-NPs with spherical morphology were incorporated into the composite scaffold and exhibited a linear and sustained release of KGN within 30 days with desirable initial burst reduction (12% vs. 20%).
34044939	6	45	theme	good	1220:1223	arg1	candidate					1225:1233	a good candidate	1218:1233	a good candidate for tissue regeneration	1218:1257	Overall, according to the enhanced mechanical features, suitable environment for cellular growth, and sustained drug release, the optimized scaffold would be a good candidate for tissue regeneration.
34044939	6	45	theme	good	1220:1223	arg1	scaffold					1200:1207	the optimized scaffold	1186:1207	the optimized scaffold	1186:1207	Overall, according to the enhanced mechanical features, suitable environment for cellular growth, and sustained drug release, the optimized scaffold would be a good candidate for tissue regeneration.
34044939	6	46	theme	cellular	1141:1148	arg1	growth					1150:1155	cellular growth	1141:1155	cellular growth	1141:1155	Overall, according to the enhanced mechanical features, suitable environment for cellular growth, and sustained drug release, the optimized scaffold would be a good candidate for tissue regeneration.
34044939	0	47	theme	controlled	66:75	arg1	delivery					88:95	controlled Kartogenin delivery	66:95	controlled Kartogenin delivery for tissue engineering	66:118	Alginate sulfate-based hydrogel/nanofiber composite scaffold with controlled Kartogenin delivery for tissue engineering.
34044939	2	48	theme	swelling	570:577	arg1	ratio					579:583	excellent swelling ratio	560:583	excellent swelling ratio	560:583	The optimized composite scaffold revealed a range of advantages such as improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability.
34044939	5	49	theme	cell	1047:1050	arg1	growth					1052:1057	cell growth	1047:1057	cell growth	1047:1057	Resazurin assay and Live/Dead staining revealed that the optimized composite scaffold has no cytotoxic effect and could improve cell growth.
34044939	3	50	dep	reduction	824:832	arg1	%					837:837	12%	835:837	12%	835:837	Furthermore, the synthesized KGN-NPs with spherical morphology were incorporated into the composite scaffold and exhibited a linear and sustained release of KGN within 30 days with desirable initial burst reduction (12% vs. 20%).
34044939	3	50	dep	reduction	824:832	arg1	%					845:845	20%	843:845	20%	843:845	Furthermore, the synthesized KGN-NPs with spherical morphology were incorporated into the composite scaffold and exhibited a linear and sustained release of KGN within 30 days with desirable initial burst reduction (12% vs. 20%).
34044939	6	51	theme	optimized	1190:1198	arg1	candidate					1225:1233	a good candidate	1218:1233	a good candidate for tissue regeneration	1218:1257	Overall, according to the enhanced mechanical features, suitable environment for cellular growth, and sustained drug release, the optimized scaffold would be a good candidate for tissue regeneration.
34044939	6	51	theme	optimized	1190:1198	arg1	scaffold					1200:1207	the optimized scaffold	1186:1207	the optimized scaffold	1186:1207	Overall, according to the enhanced mechanical features, suitable environment for cellular growth, and sustained drug release, the optimized scaffold would be a good candidate for tissue regeneration.
34044939	2	52	theme	excellent	560:568	arg1	ratio					579:583	excellent swelling ratio	560:583	excellent swelling ratio	560:583	The optimized composite scaffold revealed a range of advantages such as improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability.
34044939	2	53	theme	composite	343:351	arg1	scaffold					353:360	The optimized composite scaffold	329:360	The optimized composite scaffold	329:360	The optimized composite scaffold revealed a range of advantages such as improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability.
34044939	4	54	theme	composite	894:902	arg1	impact					880:885	the cytotoxicity impact	863:885	the cytotoxicity impact of the composite	863:902	Additionally, the cytotoxicity impact of the composite was evaluated.
34044939	2	55	theme	optimized	333:341	arg1	scaffold					353:360	The optimized composite scaffold	329:360	The optimized composite scaffold	329:360	The optimized composite scaffold revealed a range of advantages such as improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability.
34044939	5	56	theme	Resazurin	919:927	arg1	assay					929:933	Resazurin assay	919:933	Resazurin assay	919:933	Resazurin assay and Live/Dead staining revealed that the optimized composite scaffold has no cytotoxic effect and could improve cell growth.
34044939	6	57	theme	drug	1172:1175	arg1	release					1177:1183	sustained drug release	1162:1183	sustained drug release	1162:1183	Overall, according to the enhanced mechanical features, suitable environment for cellular growth, and sustained drug release, the optimized scaffold would be a good candidate for tissue regeneration.
34044939	2	58	theme	advantages	382:391	arg1	range					373:377	a range	371:377	a range of advantages such as improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability	371:616	The optimized composite scaffold revealed a range of advantages such as improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability.
34044939	2	58	theme	advantages	382:391	arg1	features					421:428	improved mechanical features	401:428	improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability	401:616	The optimized composite scaffold revealed a range of advantages such as improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability.
34044939	2	59	theme	improved	401:408	arg1	features					421:428	improved mechanical features	401:428	improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability	401:616	The optimized composite scaffold revealed a range of advantages such as improved mechanical features as well as less potential of damage (less dissipated energy), interconnected pores of hydrogel and fiber with adequate pore size, excellent swelling ratio, and controlled biodegradability.
34044939	3	60	theme	initial	810:816	arg1	reduction					824:832	desirable initial burst reduction	800:832	desirable initial burst reduction (12% vs. 20%)	800:846	Furthermore, the synthesized KGN-NPs with spherical morphology were incorporated into the composite scaffold and exhibited a linear and sustained release of KGN within 30 days with desirable initial burst reduction (12% vs. 20%).
34044939	5	61	theme	cytotoxic	1012:1020	arg1	effect					1022:1027	no cytotoxic effect	1009:1027	no cytotoxic effect	1009:1027	Resazurin assay and Live/Dead staining revealed that the optimized composite scaffold has no cytotoxic effect and could improve cell growth.
34044939	3	62	theme	burst	818:822	arg1	reduction					824:832	desirable initial burst reduction	800:832	desirable initial burst reduction (12% vs. 20%)	800:846	Furthermore, the synthesized KGN-NPs with spherical morphology were incorporated into the composite scaffold and exhibited a linear and sustained release of KGN within 30 days with desirable initial burst reduction (12% vs. 20%).
33352657	6	0	theme	waste	831:835	arg1	material					841:848	waste raw material	831:848	waste raw material	831:848	Therefore, it is necessary to characterize the main determinants of the quality defects prior to the baking process in order to predict baking quality and not waste raw material, energy, and time.
33352657	1	1	theme	rye	135:137	arg1	products					147:154	rye milling products	135:154	rye milling products	135:154	For decades, the evaluation of rye milling products have been aimed at detecting raw material defects that are linked to excessive enzyme activity.
33352657	4	2	theme	compound	538:545	arg1	composition					547:557	compound composition	538:557	compound composition in rye	538:564	A further problem is the continuing climate change, which affects compound composition in rye.
33352657	0	3	theme	Factors	65:71	arg1	Analysis					19:26	Analysis	19:26	Rye Bread Defects: Analysis of Composition and Further Influence Factors as Determinants of Dry-Baking.	0:102	Rye Bread Defects: Analysis of Composition and Further Influence Factors as Determinants of Dry-Baking.
33352657	4	4	theme	further	474:480	arg1	change					516:521	the continuing climate change	493:521	the continuing climate change	493:521	A further problem is the continuing climate change, which affects compound composition in rye.
33352657	4	4	theme	further	474:480	arg1	problem					482:488	A further problem	472:488	A further problem	472:488	A further problem is the continuing climate change, which affects compound composition in rye.
33352657	7	5	theme	major	1011:1015	arg1	role					1017:1020	a major role	1009:1020	a major role	1009:1020	In this study, it was found that the water accessibility of starch for gelatinization and its partial inhibition by certain components play a major role in baking quality.
33352657	6	6	theme	quality	744:750	arg1	defects					752:758	the quality defects	740:758	the quality defects	740:758	Therefore, it is necessary to characterize the main determinants of the quality defects prior to the baking process in order to predict baking quality and not waste raw material, energy, and time.
33352657	1	7	theme	milling	139:145	arg1	products					147:154	rye milling products	135:154	rye milling products	135:154	For decades, the evaluation of rye milling products have been aimed at detecting raw material defects that are linked to excessive enzyme activity.
33352657	1	8	theme	excessive	225:233	arg1	activity					242:249	excessive enzyme activity	225:249	excessive enzyme activity	225:249	For decades, the evaluation of rye milling products have been aimed at detecting raw material defects that are linked to excessive enzyme activity.
33352657	5	9	theme	resting	662:668	arg1	dough					656:660	extended dough resting	647:668	extended dough resting	647:668	So far, these bread defects can only be corrected by process engineering (e.g., extended dough resting).
33352657	9	10	theme	reliable	1399:1406	arg1	conclusions					1408:1418	any reliable conclusions	1395:1418	any reliable conclusions about rye flour suitability for use as bread rye	1395:1467	However, traditional quantitative analysis of the ingredients and properties of the rye milling products (e.g., falling number, protein content, amylographic data) does not allow any reliable conclusions about rye flour suitability for use as bread rye.
33352657	10	11	theme	compositional	1508:1520	arg1	aspects					1522:1528	more complex compositional aspects	1495:1528	more complex compositional aspects (e.g., complexation of compounds)	1495:1562	It can be concluded that more complex compositional aspects (e.g., complexation of compounds) need to be characterized for future quality control of rye.
33352657	1	12	theme	enzyme	235:240	arg1	activity					242:249	excessive enzyme activity	225:249	excessive enzyme activity	225:249	For decades, the evaluation of rye milling products have been aimed at detecting raw material defects that are linked to excessive enzyme activity.
33352657	4	13	from	composition	547:557	arg1	rye					562:564	rye	562:564	rye	562:564	A further problem is the continuing climate change, which affects compound composition in rye.
33352657	10	14	theme	quality	1600:1606	arg1	control					1608:1614	future quality control	1593:1614	future quality control of rye	1593:1621	It can be concluded that more complex compositional aspects (e.g., complexation of compounds) need to be characterized for future quality control of rye.
33352657	3	15	theme	present	449:455	arg1	flours					442:447	all rye flours	434:447	all rye flours present on the market	434:469	However, such methods do not sufficiently reflect the baking properties of all rye flours present on the market.
33352657	7	16	located	found	891:895	arg2	it					884:885	it	884:885	it	884:885	In this study, it was found that the water accessibility of starch for gelatinization and its partial inhibition by certain components play a major role in baking quality.
33352657	7	16	located	found	891:895	arg1	study					877:881	this study	872:881	this study	872:881	In this study, it was found that the water accessibility of starch for gelatinization and its partial inhibition by certain components play a major role in baking quality.
33352657	6	17	theme	baking	808:813	arg1	quality					815:821	baking quality	808:821	baking quality	808:821	Therefore, it is necessary to characterize the main determinants of the quality defects prior to the baking process in order to predict baking quality and not waste raw material, energy, and time.
33352657	8	18	theme	high	1055:1058	arg1	denaturation					1130:1141	a hindered denaturation	1119:1141	a hindered denaturation of proteins	1119:1153	Specifically, high amounts of insoluble nonstarch-polysaccharides (NSPSs) and a hindered denaturation of proteins seem to be an indication and reason for poor baking quality.
33352657	8	18	theme	high	1055:1058	arg1	NSPSs					1108:1112	NSPSs	1108:1112	NSPSs	1108:1112	Specifically, high amounts of insoluble nonstarch-polysaccharides (NSPSs) and a hindered denaturation of proteins seem to be an indication and reason for poor baking quality.
33352657	8	18	theme	high	1055:1058	arg1	amounts					1060:1066	high amounts	1055:1066	high amounts of insoluble nonstarch-polysaccharides (NSPSs) and a hindered denaturation of proteins	1055:1153	Specifically, high amounts of insoluble nonstarch-polysaccharides (NSPSs) and a hindered denaturation of proteins seem to be an indication and reason for poor baking quality.
33352657	8	18	theme	high	1055:1058	arg1	nonstarch-polysaccharides					1081:1105	insoluble nonstarch-polysaccharides	1071:1105	insoluble nonstarch-polysaccharides (NSPSs)	1071:1113	Specifically, high amounts of insoluble nonstarch-polysaccharides (NSPSs) and a hindered denaturation of proteins seem to be an indication and reason for poor baking quality.
33352657	8	19	theme	proteins	1146:1153	arg1	denaturation					1130:1141	a hindered denaturation	1119:1141	a hindered denaturation of proteins	1119:1153	Specifically, high amounts of insoluble nonstarch-polysaccharides (NSPSs) and a hindered denaturation of proteins seem to be an indication and reason for poor baking quality.
33352657	8	19	theme	proteins	1146:1153	arg1	NSPSs					1108:1112	NSPSs	1108:1112	NSPSs	1108:1112	Specifically, high amounts of insoluble nonstarch-polysaccharides (NSPSs) and a hindered denaturation of proteins seem to be an indication and reason for poor baking quality.
33352657	8	19	theme	proteins	1146:1153	arg1	nonstarch-polysaccharides					1081:1105	insoluble nonstarch-polysaccharides	1071:1105	insoluble nonstarch-polysaccharides (NSPSs)	1071:1113	Specifically, high amounts of insoluble nonstarch-polysaccharides (NSPSs) and a hindered denaturation of proteins seem to be an indication and reason for poor baking quality.
33352657	9	20	theme	products	1312:1319	arg1	properties					1282:1291	properties	1282:1291	properties	1282:1291	However, traditional quantitative analysis of the ingredients and properties of the rye milling products (e.g., falling number, protein content, amylographic data) does not allow any reliable conclusions about rye flour suitability for use as bread rye.
33352657	9	20	theme	products	1312:1319	arg1	ingredients					1266:1276	ingredients	1266:1276	ingredients	1266:1276	However, traditional quantitative analysis of the ingredients and properties of the rye milling products (e.g., falling number, protein content, amylographic data) does not allow any reliable conclusions about rye flour suitability for use as bread rye.
33352657	1	21	theme	products	147:154	arg1	evaluation					121:130	the evaluation	117:130	the evaluation of rye milling products	117:154	For decades, the evaluation of rye milling products have been aimed at detecting raw material defects that are linked to excessive enzyme activity.
33352657	0	22	theme	Dry-Baking	92:101	arg1	Determinants					76:87	Determinants	76:87	Determinants of Dry-Baking	76:101	Rye Bread Defects: Analysis of Composition and Further Influence Factors as Determinants of Dry-Baking.
33352657	7	23	theme	certain	985:991	arg1	components					993:1002	certain components	985:1002	certain components	985:1002	In this study, it was found that the water accessibility of starch for gelatinization and its partial inhibition by certain components play a major role in baking quality.
33352657	6	24	theme	main	719:722	arg1	determinants					724:735	the main determinants	715:735	the main determinants of the quality defects prior to the baking process in order to predict baking quality and not waste raw material, energy, and time	715:866	Therefore, it is necessary to characterize the main determinants of the quality defects prior to the baking process in order to predict baking quality and not waste raw material, energy, and time.
33352657	9	25	theme	rye	1426:1428	arg1	suitability					1436:1446	rye flour suitability	1426:1446	rye flour suitability for use as bread rye	1426:1467	However, traditional quantitative analysis of the ingredients and properties of the rye milling products (e.g., falling number, protein content, amylographic data) does not allow any reliable conclusions about rye flour suitability for use as bread rye.
33352657	3	26	theme	baking	413:418	arg1	properties					420:429	the baking properties	409:429	the baking properties of all rye flours present on the market	409:469	However, such methods do not sufficiently reflect the baking properties of all rye flours present on the market.
33352657	0	27	theme	Bread	4:8	arg1	Defects					10:16	Rye Bread Defects	0:16	Rye Bread Defects: Analysis of Composition and Further Influence Factors as Determinants of Dry-Baking.	0:102	Rye Bread Defects: Analysis of Composition and Further Influence Factors as Determinants of Dry-Baking.
33352657	5	28	theme	bread	581:585	arg1	defects					587:593	these bread defects	575:593	these bread defects	575:593	So far, these bread defects can only be corrected by process engineering (e.g., extended dough resting).
33352657	9	29	theme	flour	1430:1434	arg1	suitability					1436:1446	rye flour suitability	1426:1446	rye flour suitability for use as bread rye	1426:1467	However, traditional quantitative analysis of the ingredients and properties of the rye milling products (e.g., falling number, protein content, amylographic data) does not allow any reliable conclusions about rye flour suitability for use as bread rye.
33352657	7	30	theme	starch	929:934	arg1	accessibility					912:924	the water accessibility	902:924	the water accessibility of starch for gelatinization	902:953	In this study, it was found that the water accessibility of starch for gelatinization and its partial inhibition by certain components play a major role in baking quality.
33352657	7	30	theme	starch	929:934	arg1	inhibition					971:980	its partial inhibition	959:980	its partial inhibition by certain components	959:1002	In this study, it was found that the water accessibility of starch for gelatinization and its partial inhibition by certain components play a major role in baking quality.
33352657	0	31	theme	Rye	0:2	arg1	Defects					10:16	Rye Bread Defects	0:16	Rye Bread Defects: Analysis of Composition and Further Influence Factors as Determinants of Dry-Baking.	0:102	Rye Bread Defects: Analysis of Composition and Further Influence Factors as Determinants of Dry-Baking.
33352657	6	32	theme	baking	773:778	arg1	process					780:786	the baking process	769:786	the baking process in order to predict baking quality and not waste raw material, energy, and time	769:866	Therefore, it is necessary to characterize the main determinants of the quality defects prior to the baking process in order to predict baking quality and not waste raw material, energy, and time.
33352657	9	33	theme	milling	1304:1310	arg1	products					1312:1319	the rye milling products	1296:1319	the rye milling products (e.g., falling number, protein content, amylographic data)	1296:1378	However, traditional quantitative analysis of the ingredients and properties of the rye milling products (e.g., falling number, protein content, amylographic data) does not allow any reliable conclusions about rye flour suitability for use as bread rye.
33352657	7	34	theme	partial	963:969	arg1	inhibition					971:980	its partial inhibition	959:980	its partial inhibition by certain components	959:1002	In this study, it was found that the water accessibility of starch for gelatinization and its partial inhibition by certain components play a major role in baking quality.
33352657	2	35	theme	products	349:356	arg1	methods					315:321	the rheological methods	299:321	the rheological methods of the dough or the final products	299:356	Those defects were indirectly characterized by the rheological methods of the dough or the final products.
33352657	8	36	theme	nonstarch-polysaccharides	1081:1105	arg1	denaturation					1130:1141	a hindered denaturation	1119:1141	a hindered denaturation of proteins	1119:1153	Specifically, high amounts of insoluble nonstarch-polysaccharides (NSPSs) and a hindered denaturation of proteins seem to be an indication and reason for poor baking quality.
33352657	8	36	theme	nonstarch-polysaccharides	1081:1105	arg1	NSPSs					1108:1112	NSPSs	1108:1112	NSPSs	1108:1112	Specifically, high amounts of insoluble nonstarch-polysaccharides (NSPSs) and a hindered denaturation of proteins seem to be an indication and reason for poor baking quality.
33352657	8	36	theme	nonstarch-polysaccharides	1081:1105	arg1	amounts					1060:1066	high amounts	1055:1066	high amounts of insoluble nonstarch-polysaccharides (NSPSs) and a hindered denaturation of proteins	1055:1153	Specifically, high amounts of insoluble nonstarch-polysaccharides (NSPSs) and a hindered denaturation of proteins seem to be an indication and reason for poor baking quality.
33352657	8	36	theme	nonstarch-polysaccharides	1081:1105	arg1	nonstarch-polysaccharides					1081:1105	insoluble nonstarch-polysaccharides	1071:1105	insoluble nonstarch-polysaccharides (NSPSs)	1071:1113	Specifically, high amounts of insoluble nonstarch-polysaccharides (NSPSs) and a hindered denaturation of proteins seem to be an indication and reason for poor baking quality.
33352657	4	37	theme	climate	508:514	arg1	change					516:521	the continuing climate change	493:521	the continuing climate change	493:521	A further problem is the continuing climate change, which affects compound composition in rye.
33352657	4	37	theme	climate	508:514	arg1	problem					482:488	A further problem	472:488	A further problem	472:488	A further problem is the continuing climate change, which affects compound composition in rye.
33352657	8	38	theme	baking	1200:1205	arg1	quality					1207:1213	poor baking quality	1195:1213	poor baking quality	1195:1213	Specifically, high amounts of insoluble nonstarch-polysaccharides (NSPSs) and a hindered denaturation of proteins seem to be an indication and reason for poor baking quality.
33352657	8	39	theme	insoluble	1071:1079	arg1	NSPSs					1108:1112	NSPSs	1108:1112	NSPSs	1108:1112	Specifically, high amounts of insoluble nonstarch-polysaccharides (NSPSs) and a hindered denaturation of proteins seem to be an indication and reason for poor baking quality.
33352657	8	39	theme	insoluble	1071:1079	arg1	nonstarch-polysaccharides					1081:1105	insoluble nonstarch-polysaccharides	1071:1105	insoluble nonstarch-polysaccharides (NSPSs)	1071:1113	Specifically, high amounts of insoluble nonstarch-polysaccharides (NSPSs) and a hindered denaturation of proteins seem to be an indication and reason for poor baking quality.
33352657	10	40	theme	future	1593:1598	arg1	control					1608:1614	future quality control	1593:1614	future quality control of rye	1593:1621	It can be concluded that more complex compositional aspects (e.g., complexation of compounds) need to be characterized for future quality control of rye.
33352657	2	41	theme	dough	330:334	arg1	methods					315:321	the rheological methods	299:321	the rheological methods of the dough or the final products	299:356	Those defects were indirectly characterized by the rheological methods of the dough or the final products.
33352657	3	42	theme	rye	438:440	arg1	flours					442:447	all rye flours	434:447	all rye flours present on the market	434:469	However, such methods do not sufficiently reflect the baking properties of all rye flours present on the market.
33352657	9	43	theme	traditional	1225:1235	arg1	analysis					1250:1257	traditional quantitative analysis	1225:1257	traditional quantitative analysis of the ingredients and properties of the rye milling products (e.g., falling number, protein content, amylographic data)	1225:1378	However, traditional quantitative analysis of the ingredients and properties of the rye milling products (e.g., falling number, protein content, amylographic data) does not allow any reliable conclusions about rye flour suitability for use as bread rye.
33352657	5	44	theme	extended	647:654	arg1	dough					656:660	extended dough resting	647:668	extended dough resting	647:668	So far, these bread defects can only be corrected by process engineering (e.g., extended dough resting).
33352657	9	45	theme	protein	1344:1350	arg1	number					1336:1341	number	1336:1341	number	1336:1341	However, traditional quantitative analysis of the ingredients and properties of the rye milling products (e.g., falling number, protein content, amylographic data) does not allow any reliable conclusions about rye flour suitability for use as bread rye.
33352657	9	45	theme	protein	1344:1350	arg1	content					1352:1358	protein content	1344:1358	protein content	1344:1358	However, traditional quantitative analysis of the ingredients and properties of the rye milling products (e.g., falling number, protein content, amylographic data) does not allow any reliable conclusions about rye flour suitability for use as bread rye.
33352657	8	46	theme	poor	1195:1198	arg1	quality					1207:1213	poor baking quality	1195:1213	poor baking quality	1195:1213	Specifically, high amounts of insoluble nonstarch-polysaccharides (NSPSs) and a hindered denaturation of proteins seem to be an indication and reason for poor baking quality.
33352657	8	47	theme	denaturation	1130:1141	arg1	denaturation					1130:1141	a hindered denaturation	1119:1141	a hindered denaturation of proteins	1119:1153	Specifically, high amounts of insoluble nonstarch-polysaccharides (NSPSs) and a hindered denaturation of proteins seem to be an indication and reason for poor baking quality.
33352657	8	47	theme	denaturation	1130:1141	arg1	NSPSs					1108:1112	NSPSs	1108:1112	NSPSs	1108:1112	Specifically, high amounts of insoluble nonstarch-polysaccharides (NSPSs) and a hindered denaturation of proteins seem to be an indication and reason for poor baking quality.
33352657	8	47	theme	denaturation	1130:1141	arg1	amounts					1060:1066	high amounts	1055:1066	high amounts of insoluble nonstarch-polysaccharides (NSPSs) and a hindered denaturation of proteins	1055:1153	Specifically, high amounts of insoluble nonstarch-polysaccharides (NSPSs) and a hindered denaturation of proteins seem to be an indication and reason for poor baking quality.
33352657	8	47	theme	denaturation	1130:1141	arg1	nonstarch-polysaccharides					1081:1105	insoluble nonstarch-polysaccharides	1071:1105	insoluble nonstarch-polysaccharides (NSPSs)	1071:1113	Specifically, high amounts of insoluble nonstarch-polysaccharides (NSPSs) and a hindered denaturation of proteins seem to be an indication and reason for poor baking quality.
33352657	1	48	theme	raw	185:187	arg1	defects					198:204	raw material defects	185:204	raw material defects that are linked to excessive enzyme activity	185:249	For decades, the evaluation of rye milling products have been aimed at detecting raw material defects that are linked to excessive enzyme activity.
33352657	8	49	theme	hindered	1121:1128	arg1	denaturation					1130:1141	a hindered denaturation	1119:1141	a hindered denaturation of proteins	1119:1153	Specifically, high amounts of insoluble nonstarch-polysaccharides (NSPSs) and a hindered denaturation of proteins seem to be an indication and reason for poor baking quality.
33352657	9	50	theme	quantitative	1237:1248	arg1	analysis					1250:1257	traditional quantitative analysis	1225:1257	traditional quantitative analysis of the ingredients and properties of the rye milling products (e.g., falling number, protein content, amylographic data)	1225:1378	However, traditional quantitative analysis of the ingredients and properties of the rye milling products (e.g., falling number, protein content, amylographic data) does not allow any reliable conclusions about rye flour suitability for use as bread rye.
33352657	9	51	theme	rye	1300:1302	arg1	products					1312:1319	the rye milling products	1296:1319	the rye milling products (e.g., falling number, protein content, amylographic data)	1296:1378	However, traditional quantitative analysis of the ingredients and properties of the rye milling products (e.g., falling number, protein content, amylographic data) does not allow any reliable conclusions about rye flour suitability for use as bread rye.
33352657	9	52	dep	ingredients	1266:1276	arg1	the					1262:1264	the	1262:1264	the	1262:1264	However, traditional quantitative analysis of the ingredients and properties of the rye milling products (e.g., falling number, protein content, amylographic data) does not allow any reliable conclusions about rye flour suitability for use as bread rye.
33352657	9	53	theme	bread	1459:1463	arg1	rye					1465:1467	bread rye	1459:1467	bread rye	1459:1467	However, traditional quantitative analysis of the ingredients and properties of the rye milling products (e.g., falling number, protein content, amylographic data) does not allow any reliable conclusions about rye flour suitability for use as bread rye.
33352657	1	54	theme	material	189:196	arg1	defects					198:204	raw material defects	185:204	raw material defects that are linked to excessive enzyme activity	185:249	For decades, the evaluation of rye milling products have been aimed at detecting raw material defects that are linked to excessive enzyme activity.
33352657	3	55	theme	such	368:371	arg1	methods					373:379	such methods	368:379	such methods	368:379	However, such methods do not sufficiently reflect the baking properties of all rye flours present on the market.
33352657	5	56	dep	dough	656:660	arg1	e.g.					641:644	e.g.	641:644	e.g.	641:644	So far, these bread defects can only be corrected by process engineering (e.g., extended dough resting).
33352657	1	57	attach	linked	215:220	arg2	defects					198:204	raw material defects	185:204	raw material defects that are linked to excessive enzyme activity	185:249	For decades, the evaluation of rye milling products have been aimed at detecting raw material defects that are linked to excessive enzyme activity.
33352657	1	57	attach	linked	215:220	arg1	activity					242:249	excessive enzyme activity	225:249	excessive enzyme activity	225:249	For decades, the evaluation of rye milling products have been aimed at detecting raw material defects that are linked to excessive enzyme activity.
33352657	0	58	theme	Composition	31:41	arg1	Factors					65:71	Composition and Further Influence Factors	31:71	Composition and Further Influence Factors as Determinants of Dry-Baking	31:101	Rye Bread Defects: Analysis of Composition and Further Influence Factors as Determinants of Dry-Baking.
33352657	4	59	theme	continuing	497:506	arg1	change					516:521	the continuing climate change	493:521	the continuing climate change	493:521	A further problem is the continuing climate change, which affects compound composition in rye.
33352657	4	59	theme	continuing	497:506	arg1	problem					482:488	A further problem	472:488	A further problem	472:488	A further problem is the continuing climate change, which affects compound composition in rye.
33352657	5	60	theme	process	620:626	arg1	engineering					628:638	process engineering	620:638	process engineering (e.g., extended dough resting)	620:669	So far, these bread defects can only be corrected by process engineering (e.g., extended dough resting).
33352657	10	61	theme	compounds	1553:1561	arg1	complexation					1537:1548	complexation	1537:1548	complexation of compounds	1537:1561	It can be concluded that more complex compositional aspects (e.g., complexation of compounds) need to be characterized for future quality control of rye.
33352657	9	62	theme	amylographic	1361:1372	arg1	data					1374:1377	amylographic data	1361:1377	amylographic data	1361:1377	However, traditional quantitative analysis of the ingredients and properties of the rye milling products (e.g., falling number, protein content, amylographic data) does not allow any reliable conclusions about rye flour suitability for use as bread rye.
33352657	9	62	theme	amylographic	1361:1372	arg1	number					1336:1341	number	1336:1341	number	1336:1341	However, traditional quantitative analysis of the ingredients and properties of the rye milling products (e.g., falling number, protein content, amylographic data) does not allow any reliable conclusions about rye flour suitability for use as bread rye.
33352657	7	63	theme	water	906:910	arg1	accessibility					912:924	the water accessibility	902:924	the water accessibility of starch for gelatinization	902:953	In this study, it was found that the water accessibility of starch for gelatinization and its partial inhibition by certain components play a major role in baking quality.
33352657	9	64	dep	falling	1328:1334	arg1	e.g.					1322:1325	e.g.	1322:1325	e.g.	1322:1325	However, traditional quantitative analysis of the ingredients and properties of the rye milling products (e.g., falling number, protein content, amylographic data) does not allow any reliable conclusions about rye flour suitability for use as bread rye.
33352657	3	65	theme	flours	442:447	arg1	properties					420:429	the baking properties	409:429	the baking properties of all rye flours present on the market	409:469	However, such methods do not sufficiently reflect the baking properties of all rye flours present on the market.
33352657	10	66	theme	rye	1619:1621	arg1	control					1608:1614	future quality control	1593:1614	future quality control of rye	1593:1621	It can be concluded that more complex compositional aspects (e.g., complexation of compounds) need to be characterized for future quality control of rye.
33352657	9	67	theme	ingredients	1266:1276	arg1	analysis					1250:1257	traditional quantitative analysis	1225:1257	traditional quantitative analysis of the ingredients and properties of the rye milling products (e.g., falling number, protein content, amylographic data)	1225:1378	However, traditional quantitative analysis of the ingredients and properties of the rye milling products (e.g., falling number, protein content, amylographic data) does not allow any reliable conclusions about rye flour suitability for use as bread rye.
33352657	10	68	dep	complexation	1537:1548	arg1	e.g.					1531:1534	e.g.	1531:1534	e.g.	1531:1534	It can be concluded that more complex compositional aspects (e.g., complexation of compounds) need to be characterized for future quality control of rye.
33352657	0	69	dep	Defects	10:16	arg1	Analysis					19:26	Analysis	19:26	Rye Bread Defects: Analysis of Composition and Further Influence Factors as Determinants of Dry-Baking.	0:102	Rye Bread Defects: Analysis of Composition and Further Influence Factors as Determinants of Dry-Baking.
33352657	2	70	theme	rheological	303:313	arg1	methods					315:321	the rheological methods	299:321	the rheological methods of the dough or the final products	299:356	Those defects were indirectly characterized by the rheological methods of the dough or the final products.
33352657	0	71	theme	Influence	55:63	arg1	Factors					65:71	Composition and Further Influence Factors	31:71	Composition and Further Influence Factors as Determinants of Dry-Baking	31:101	Rye Bread Defects: Analysis of Composition and Further Influence Factors as Determinants of Dry-Baking.
33352657	6	72	theme	defects	752:758	arg1	determinants					724:735	the main determinants	715:735	the main determinants of the quality defects prior to the baking process in order to predict baking quality and not waste raw material, energy, and time	715:866	Therefore, it is necessary to characterize the main determinants of the quality defects prior to the baking process in order to predict baking quality and not waste raw material, energy, and time.
33352657	8	73	dep	indication	1169:1178	arg1	an					1166:1167	an	1166:1167	an	1166:1167	Specifically, high amounts of insoluble nonstarch-polysaccharides (NSPSs) and a hindered denaturation of proteins seem to be an indication and reason for poor baking quality.
33352657	2	74	theme	final	343:347	arg1	products					349:356	the final products	339:356	the final products	339:356	Those defects were indirectly characterized by the rheological methods of the dough or the final products.
33352657	10	75	theme	complex	1500:1506	arg1	aspects					1522:1528	more complex compositional aspects	1495:1528	more complex compositional aspects (e.g., complexation of compounds)	1495:1562	It can be concluded that more complex compositional aspects (e.g., complexation of compounds) need to be characterized for future quality control of rye.
33352657	9	76	theme	properties	1282:1291	arg1	analysis					1250:1257	traditional quantitative analysis	1225:1257	traditional quantitative analysis of the ingredients and properties of the rye milling products (e.g., falling number, protein content, amylographic data)	1225:1378	However, traditional quantitative analysis of the ingredients and properties of the rye milling products (e.g., falling number, protein content, amylographic data) does not allow any reliable conclusions about rye flour suitability for use as bread rye.
33352657	0	77	theme	Further	47:53	arg1	Influence					55:63	Further Influence	47:63	Further Influence	47:63	Rye Bread Defects: Analysis of Composition and Further Influence Factors as Determinants of Dry-Baking.
33352657	6	78	theme	raw	837:839	arg1	material					841:848	waste raw material	831:848	waste raw material	831:848	Therefore, it is necessary to characterize the main determinants of the quality defects prior to the baking process in order to predict baking quality and not waste raw material, energy, and time.
33352657	3	79	attach	present	449:455	arg2	flours					442:447	all rye flours	434:447	all rye flours present on the market	434:469	However, such methods do not sufficiently reflect the baking properties of all rye flours present on the market.
33352657	3	79	attach	present	449:455	arg1	market					464:469	the market	460:469	the market	460:469	However, such methods do not sufficiently reflect the baking properties of all rye flours present on the market.
33639433	7	0	theme	interfacial	902:912	arg1	composition					914:924	interfacial composition	902:924	interfacial composition	902:924	Moreover, this binding appears to be reinforced under gastric conditions, hence evidencing the crucial role of interfacial composition in protecting encapsulated curcumin.
33639433	4	1	theme	in	559:560	arg1	protocol					578:585	a standard in vitro digestion protocol	548:585	a standard in vitro digestion protocol	548:585	Bioaccessibility of curcumin is evaluated following a standard in vitro digestion protocol.
33639433	5	2	theme	~85	720:722	arg1	%					715:715	%	715:715	%	715:715	The presence of HA in the shell increases the amount of curcumin retained in the LLNs after in vitro gastric digestion from ~25% to ~85%.
33639433	5	3	from	%	723:723	arg1	digestion					697:705	in vitro gastric digestion	680:705	in vitro gastric digestion from ~25% to ~85%	680:723	The presence of HA in the shell increases the amount of curcumin retained in the LLNs after in vitro gastric digestion from ~25% to ~85%.
33639433	8	4	theme	Interfacial	963:973	arg1	engineering					975:985	Interfacial engineering	963:985	Interfacial engineering of nanoemulsions	963:1002	Interfacial engineering of nanoemulsions provides a route to improve the bioaccessibility of encapsulated curcumin at different stages in the gut.
33639433	7	5	theme	crucial	886:892	arg1	role					894:897	the crucial role	882:897	the crucial role of interfacial composition in protecting encapsulated curcumin	882:960	Moreover, this binding appears to be reinforced under gastric conditions, hence evidencing the crucial role of interfacial composition in protecting encapsulated curcumin.
33639433	8	6	from	stages	1091:1096	arg1	gut					1105:1107	the gut	1101:1107	the gut	1101:1107	Interfacial engineering of nanoemulsions provides a route to improve the bioaccessibility of encapsulated curcumin at different stages in the gut.
33639433	4	7	theme	standard	550:557	arg1	protocol					578:585	a standard in vitro digestion protocol	548:585	a standard in vitro digestion protocol	548:585	Bioaccessibility of curcumin is evaluated following a standard in vitro digestion protocol.
33639433	3	8	theme	oil	364:366	arg1	emulsification					368:381	olive oil emulsification	358:381	olive oil emulsification	358:381	Here, we present curcumin-loaded liquid lipid nanocapsules (LLNs), obtained through olive oil emulsification, in which LLNs are coated by a protective shell composed of Bovine Serum Albumin (BSA) and hyaluronic acid (HA).
33639433	8	9	theme	nanoemulsions	990:1002	arg1	engineering					975:985	Interfacial engineering	963:985	Interfacial engineering of nanoemulsions	963:1002	Interfacial engineering of nanoemulsions provides a route to improve the bioaccessibility of encapsulated curcumin at different stages in the gut.
33639433	4	10	theme	digestion	568:576	arg1	protocol					578:585	a standard in vitro digestion protocol	548:585	a standard in vitro digestion protocol	548:585	Bioaccessibility of curcumin is evaluated following a standard in vitro digestion protocol.
33639433	5	11	theme	gastric	689:695	arg1	digestion					697:705	in vitro gastric digestion	680:705	in vitro gastric digestion from ~25% to ~85%	680:723	The presence of HA in the shell increases the amount of curcumin retained in the LLNs after in vitro gastric digestion from ~25% to ~85%.
33639433	3	12	theme	olive	358:362	arg1	oil					364:366	olive oil	358:366	olive oil emulsification	358:381	Here, we present curcumin-loaded liquid lipid nanocapsules (LLNs), obtained through olive oil emulsification, in which LLNs are coated by a protective shell composed of Bovine Serum Albumin (BSA) and hyaluronic acid (HA).
33639433	5	13	theme	HA	604:605	arg1	presence					592:599	The presence	588:599	The presence of HA in the shell	588:618	The presence of HA in the shell increases the amount of curcumin retained in the LLNs after in vitro gastric digestion from ~25% to ~85%.
33639433	6	14	theme	protective	731:740	arg1	effect					742:747	This protective effect	726:747	This protective effect	726:747	This protective effect occurs when HA binds to BSA in the shell.
33639433	1	15	theme	low	172:174	arg1	solubility					176:185	its low solubility	168:185	its low solubility	168:185	A major challenge in delivering curcumin effectively to the gut is its low solubility.
33639433	1	15	theme	low	172:174	arg1	challenge					109:117	A major challenge	101:117	A major challenge in delivering curcumin effectively to the gut	101:163	A major challenge in delivering curcumin effectively to the gut is its low solubility.
33639433	8	16	theme	encapsulated	1056:1067	arg1	curcumin					1069:1076	encapsulated curcumin	1056:1076	encapsulated curcumin	1056:1076	Interfacial engineering of nanoemulsions provides a route to improve the bioaccessibility of encapsulated curcumin at different stages in the gut.
33639433	3	17	theme	protective	414:423	arg1	shell					425:429	a protective shell	412:429	a protective shell composed of Bovine Serum Albumin (BSA) and hyaluronic acid (HA)	412:493	Here, we present curcumin-loaded liquid lipid nanocapsules (LLNs), obtained through olive oil emulsification, in which LLNs are coated by a protective shell composed of Bovine Serum Albumin (BSA) and hyaluronic acid (HA).
33639433	7	18	theme	gastric	845:851	arg1	conditions					853:862	gastric conditions	845:862	gastric conditions	845:862	Moreover, this binding appears to be reinforced under gastric conditions, hence evidencing the crucial role of interfacial composition in protecting encapsulated curcumin.
33639433	5	19	attach	presence	592:599	arg1	shell					614:618	the shell	610:618	the shell	610:618	The presence of HA in the shell increases the amount of curcumin retained in the LLNs after in vitro gastric digestion from ~25% to ~85%.
33639433	5	19	attach	presence	592:599	arg2	HA					604:605	HA	604:605	HA	604:605	The presence of HA in the shell increases the amount of curcumin retained in the LLNs after in vitro gastric digestion from ~25% to ~85%.
33639433	1	20	theme	major	103:107	arg1	solubility					176:185	its low solubility	168:185	its low solubility	168:185	A major challenge in delivering curcumin effectively to the gut is its low solubility.
33639433	1	20	theme	major	103:107	arg1	challenge					109:117	A major challenge	101:117	A major challenge in delivering curcumin effectively to the gut	101:163	A major challenge in delivering curcumin effectively to the gut is its low solubility.
33639433	3	21	theme	curcumin-loaded	291:305	arg1	LLNs					334:337	LLNs	334:337	LLNs	334:337	Here, we present curcumin-loaded liquid lipid nanocapsules (LLNs), obtained through olive oil emulsification, in which LLNs are coated by a protective shell composed of Bovine Serum Albumin (BSA) and hyaluronic acid (HA).
33639433	3	21	theme	curcumin-loaded	291:305	arg1	nanocapsules					320:331	curcumin-loaded liquid lipid nanocapsules	291:331	curcumin-loaded liquid lipid nanocapsules (LLNs)	291:338	Here, we present curcumin-loaded liquid lipid nanocapsules (LLNs), obtained through olive oil emulsification, in which LLNs are coated by a protective shell composed of Bovine Serum Albumin (BSA) and hyaluronic acid (HA).
33639433	0	22	from	nanoemulsions	86:98	arg1	bioaccessibility					64:79	curcumin bioaccessibility	55:79	curcumin bioaccessibility from nanoemulsions	55:98	Investigating the role of hyaluronic acid in improving curcumin bioaccessibility from nanoemulsions.
33639433	0	23	theme	acid	37:40	arg1	role					18:21	the role	14:21	the role of hyaluronic acid in improving curcumin bioaccessibility from nanoemulsions	14:98	Investigating the role of hyaluronic acid in improving curcumin bioaccessibility from nanoemulsions.
33639433	3	24	theme	Bovine	443:448	arg1	BSA					465:467	BSA	465:467	BSA	465:467	Here, we present curcumin-loaded liquid lipid nanocapsules (LLNs), obtained through olive oil emulsification, in which LLNs are coated by a protective shell composed of Bovine Serum Albumin (BSA) and hyaluronic acid (HA).
33639433	3	24	theme	Bovine	443:448	arg1	Albumin					456:462	Bovine Serum Albumin	443:462	Bovine Serum Albumin (BSA)	443:468	Here, we present curcumin-loaded liquid lipid nanocapsules (LLNs), obtained through olive oil emulsification, in which LLNs are coated by a protective shell composed of Bovine Serum Albumin (BSA) and hyaluronic acid (HA).
33639433	0	25	theme	hyaluronic	26:35	arg1	acid					37:40	hyaluronic acid	26:40	hyaluronic acid	26:40	Investigating the role of hyaluronic acid in improving curcumin bioaccessibility from nanoemulsions.
33639433	5	26	theme	in	680:681	arg1	digestion					697:705	in vitro gastric digestion	680:705	in vitro gastric digestion from ~25% to ~85%	680:723	The presence of HA in the shell increases the amount of curcumin retained in the LLNs after in vitro gastric digestion from ~25% to ~85%.
33639433	4	27	dep	in	559:560	arg1	vitro					562:566	vitro	562:566	vitro	562:566	Bioaccessibility of curcumin is evaluated following a standard in vitro digestion protocol.
33639433	3	28	theme	Serum	450:454	arg1	BSA					465:467	BSA	465:467	BSA	465:467	Here, we present curcumin-loaded liquid lipid nanocapsules (LLNs), obtained through olive oil emulsification, in which LLNs are coated by a protective shell composed of Bovine Serum Albumin (BSA) and hyaluronic acid (HA).
33639433	3	28	theme	Serum	450:454	arg1	Albumin					456:462	Bovine Serum Albumin	443:462	Bovine Serum Albumin (BSA)	443:468	Here, we present curcumin-loaded liquid lipid nanocapsules (LLNs), obtained through olive oil emulsification, in which LLNs are coated by a protective shell composed of Bovine Serum Albumin (BSA) and hyaluronic acid (HA).
33639433	8	29	theme	curcumin	1069:1076	arg1	bioaccessibility					1036:1051	the bioaccessibility	1032:1051	the bioaccessibility of encapsulated curcumin	1032:1076	Interfacial engineering of nanoemulsions provides a route to improve the bioaccessibility of encapsulated curcumin at different stages in the gut.
33639433	5	30	dep	in	680:681	arg1	vitro					683:687	vitro	683:687	vitro	683:687	The presence of HA in the shell increases the amount of curcumin retained in the LLNs after in vitro gastric digestion from ~25% to ~85%.
33639433	2	31	theme	curcumin	225:232	arg1	bioaccessibility					234:249	curcumin bioaccessibility	225:249	curcumin bioaccessibility	225:249	One interesting approach to increase curcumin bioaccessibility is its emulsification.
33639433	8	32	theme	different	1081:1089	arg1	stages					1091:1096	different stages	1081:1096	different stages in the gut	1081:1107	Interfacial engineering of nanoemulsions provides a route to improve the bioaccessibility of encapsulated curcumin at different stages in the gut.
33639433	5	33	dep	%	715:715	arg1	to					717:718	to	717:718	to	717:718	The presence of HA in the shell increases the amount of curcumin retained in the LLNs after in vitro gastric digestion from ~25% to ~85%.
33639433	4	34	theme	curcumin	516:523	arg1	Bioaccessibility					496:511	Bioaccessibility	496:511	Bioaccessibility of curcumin	496:523	Bioaccessibility of curcumin is evaluated following a standard in vitro digestion protocol.
33639433	5	35	theme	curcumin	644:651	arg1	curcumin					644:651	curcumin	644:651	curcumin	644:651	The presence of HA in the shell increases the amount of curcumin retained in the LLNs after in vitro gastric digestion from ~25% to ~85%.
33639433	5	35	theme	curcumin	644:651	arg1	amount					634:639	the amount	630:639	the amount of curcumin retained in the LLNs after in vitro gastric digestion from ~25% to ~85%	630:723	The presence of HA in the shell increases the amount of curcumin retained in the LLNs after in vitro gastric digestion from ~25% to ~85%.
33639433	3	36	theme	hyaluronic	474:483	arg1	HA					491:492	HA	491:492	HA	491:492	Here, we present curcumin-loaded liquid lipid nanocapsules (LLNs), obtained through olive oil emulsification, in which LLNs are coated by a protective shell composed of Bovine Serum Albumin (BSA) and hyaluronic acid (HA).
33639433	3	36	theme	hyaluronic	474:483	arg1	acid					485:488	hyaluronic acid	474:488	hyaluronic acid (HA)	474:493	Here, we present curcumin-loaded liquid lipid nanocapsules (LLNs), obtained through olive oil emulsification, in which LLNs are coated by a protective shell composed of Bovine Serum Albumin (BSA) and hyaluronic acid (HA).
33639433	3	37	theme	liquid	307:312	arg1	LLNs					334:337	LLNs	334:337	LLNs	334:337	Here, we present curcumin-loaded liquid lipid nanocapsules (LLNs), obtained through olive oil emulsification, in which LLNs are coated by a protective shell composed of Bovine Serum Albumin (BSA) and hyaluronic acid (HA).
33639433	3	37	theme	liquid	307:312	arg1	nanocapsules					320:331	curcumin-loaded liquid lipid nanocapsules	291:331	curcumin-loaded liquid lipid nanocapsules (LLNs)	291:338	Here, we present curcumin-loaded liquid lipid nanocapsules (LLNs), obtained through olive oil emulsification, in which LLNs are coated by a protective shell composed of Bovine Serum Albumin (BSA) and hyaluronic acid (HA).
33639433	7	38	theme	encapsulated	940:951	arg1	curcumin					953:960	encapsulated curcumin	940:960	encapsulated curcumin	940:960	Moreover, this binding appears to be reinforced under gastric conditions, hence evidencing the crucial role of interfacial composition in protecting encapsulated curcumin.
33639433	3	39	theme	lipid	314:318	arg1	LLNs					334:337	LLNs	334:337	LLNs	334:337	Here, we present curcumin-loaded liquid lipid nanocapsules (LLNs), obtained through olive oil emulsification, in which LLNs are coated by a protective shell composed of Bovine Serum Albumin (BSA) and hyaluronic acid (HA).
33639433	3	39	theme	lipid	314:318	arg1	nanocapsules					320:331	curcumin-loaded liquid lipid nanocapsules	291:331	curcumin-loaded liquid lipid nanocapsules (LLNs)	291:338	Here, we present curcumin-loaded liquid lipid nanocapsules (LLNs), obtained through olive oil emulsification, in which LLNs are coated by a protective shell composed of Bovine Serum Albumin (BSA) and hyaluronic acid (HA).
33639433	2	40	theme	interesting	192:202	arg1	approach					204:211	One interesting approach	188:211	One interesting approach to increase curcumin bioaccessibility	188:249	One interesting approach to increase curcumin bioaccessibility is its emulsification.
33639433	7	41	theme	composition	914:924	arg1	role					894:897	the crucial role	882:897	the crucial role of interfacial composition in protecting encapsulated curcumin	882:960	Moreover, this binding appears to be reinforced under gastric conditions, hence evidencing the crucial role of interfacial composition in protecting encapsulated curcumin.
33639433	5	42	theme	~25	712:714	arg1	%					715:715	%	715:715	%	715:715	The presence of HA in the shell increases the amount of curcumin retained in the LLNs after in vitro gastric digestion from ~25% to ~85%.
33639433	0	43	theme	curcumin	55:62	arg1	bioaccessibility					64:79	curcumin bioaccessibility	55:79	curcumin bioaccessibility from nanoemulsions	55:98	Investigating the role of hyaluronic acid in improving curcumin bioaccessibility from nanoemulsions.
33639433	5	44	from	presence	592:599	arg1	shell					614:618	the shell	610:618	the shell	610:618	The presence of HA in the shell increases the amount of curcumin retained in the LLNs after in vitro gastric digestion from ~25% to ~85%.
32194117	10	0	from	range	1731:1735	arg1	values					1702:1707	the recovery values	1689:1707	the recovery values of the analyte in the range of 96.6%-105.4% and the precision with relative standard deviation <5.7%	1689:1808	Therefore, the D-μ-SPE-HPLC-UV method could be used for analyzing RA in the samples of the extracts obtained from the medical plants and water with the recovery values of the analyte in the range of 96.6%-105.4% and the precision with relative standard deviation <5.7%.
32194117	9	1	theme	83.3	1535:1538	arg1	factor					1525:1530	preconcentration factor	1508:1530	preconcentration factor of 83.3	1508:1538	In addition, the enrichment factors were obtained in the range of 93.7-110.5 with preconcentration factor of 83.3.
32194117	9	2	with	93.7-110.5	1492:1501	arg1	factor					1525:1530	preconcentration factor	1508:1530	preconcentration factor of 83.3	1508:1538	In addition, the enrichment factors were obtained in the range of 93.7-110.5 with preconcentration factor of 83.3.
32194117	0	3	theme	sample	200:205	arg1	extracts					163:170	the extracts	159:170	the extracts of medical plants and water sample	159:205	Biocompatible chitosan-zinc oxide nanocomposite based dispersive micro-solid phase extraction coupled with HPLC-UV for the determination of rosmarinic acid in the extracts of medical plants and water sample.
32194117	10	4	theme	extracts	1632:1639	arg1	samples					1617:1623	the samples	1613:1623	the samples of the extracts obtained from the medical plants and water with the recovery values of the analyte in the range of 96.6%-105.4% and the precision with relative standard deviation <5.7%	1613:1808	Therefore, the D-μ-SPE-HPLC-UV method could be used for analyzing RA in the samples of the extracts obtained from the medical plants and water with the recovery values of the analyte in the range of 96.6%-105.4% and the precision with relative standard deviation <5.7%.
32194117	10	5	theme	relative	1776:1783	arg1	deviation					1794:1802	relative standard deviation	1776:1802	relative standard deviation	1776:1802	Therefore, the D-μ-SPE-HPLC-UV method could be used for analyzing RA in the samples of the extracts obtained from the medical plants and water with the recovery values of the analyte in the range of 96.6%-105.4% and the precision with relative standard deviation <5.7%.
32194117	4	6	theme	composite	827:835	arg1	CCD					845:847	CCD	845:847	CCD	845:847	The important variables (pH, biocompatible composite mass, contact time, and volume of eluent) associated with the extraction process were analyzed by the application of central composite design (CCD).
32194117	4	6	theme	composite	827:835	arg1	design					837:842	central composite design	819:842	central composite design (CCD)	819:848	The important variables (pH, biocompatible composite mass, contact time, and volume of eluent) associated with the extraction process were analyzed by the application of central composite design (CCD).
32194117	10	7	theme	medical	1659:1665	arg1	plants					1667:1672	the medical plants	1655:1672	the medical plants	1655:1672	Therefore, the D-μ-SPE-HPLC-UV method could be used for analyzing RA in the samples of the extracts obtained from the medical plants and water with the recovery values of the analyte in the range of 96.6%-105.4% and the precision with relative standard deviation <5.7%.
32194117	2	8	theme	biocompatible	547:559	arg1	phase					582:586	a solid phase	574:586	a solid phase	574:586	The dispersive micro-solid phase extraction (D-μ-SPE) of RA from the medical extract samples was investigated by using the prepared biocompatible composite as a solid phase.
32194117	2	8	theme	biocompatible	547:559	arg1	composite					561:569	the prepared biocompatible composite	534:569	the prepared biocompatible composite	534:569	The dispersive micro-solid phase extraction (D-μ-SPE) of RA from the medical extract samples was investigated by using the prepared biocompatible composite as a solid phase.
32194117	0	9	theme	rosmarinic	140:149	arg1	acid					151:154	rosmarinic acid	140:154	rosmarinic acid in the extracts of medical plants and water sample	140:205	Biocompatible chitosan-zinc oxide nanocomposite based dispersive micro-solid phase extraction coupled with HPLC-UV for the determination of rosmarinic acid in the extracts of medical plants and water sample.
32194117	1	10	theme	biocompatible	374:386	arg1	nanoparticles					355:367	chitosan‑zinc oxide nanoparticles	335:367	chitosan‑zinc oxide nanoparticles	335:367	In the present research, a procedure was described for the recovery of rosmarinic acid (RA) from medical extract samples using chitosan‑zinc oxide nanoparticles as a biocompatible nanocomposite (CS-ZnO-NC).
32194117	1	10	theme	biocompatible	374:386	arg1	CS-ZnO-NC					403:411	CS-ZnO-NC	403:411	CS-ZnO-NC	403:411	In the present research, a procedure was described for the recovery of rosmarinic acid (RA) from medical extract samples using chitosan‑zinc oxide nanoparticles as a biocompatible nanocomposite (CS-ZnO-NC).
32194117	1	10	theme	biocompatible	374:386	arg1	nanocomposite					388:400	a biocompatible nanocomposite	372:400	a biocompatible nanocomposite (CS-ZnO-NC)	372:412	In the present research, a procedure was described for the recovery of rosmarinic acid (RA) from medical extract samples using chitosan‑zinc oxide nanoparticles as a biocompatible nanocomposite (CS-ZnO-NC).
32194117	3	11	theme	HPLC-UV	593:599	arg1	method					601:606	The HPLC-UV method	589:606	The HPLC-UV method	589:606	The HPLC-UV method was used for measuring the extracted RA.
32194117	2	12	theme	solid	576:580	arg1	phase					582:586	a solid phase	574:586	a solid phase	574:586	The dispersive micro-solid phase extraction (D-μ-SPE) of RA from the medical extract samples was investigated by using the prepared biocompatible composite as a solid phase.
32194117	2	12	theme	solid	576:580	arg1	composite					561:569	the prepared biocompatible composite	534:569	the prepared biocompatible composite	534:569	The dispersive micro-solid phase extraction (D-μ-SPE) of RA from the medical extract samples was investigated by using the prepared biocompatible composite as a solid phase.
32194117	8	13	theme	obtained	1336:1343	arg1	ranges					1345:1350	the obtained ranges	1332:1350	the obtained ranges	1332:1350	The limit of detection (LOD) and quantification (LOQ) values were computed, and the obtained ranges were respectively from 0.060 to 0.089 ng mL-1 and 0.201 to 0.297 ng mL-1.
32194117	0	14	from	determination	123:135	arg1	extracts					163:170	the extracts	159:170	the extracts of medical plants and water sample	159:205	Biocompatible chitosan-zinc oxide nanocomposite based dispersive micro-solid phase extraction coupled with HPLC-UV for the determination of rosmarinic acid in the extracts of medical plants and water sample.
32194117	2	15	from	samples	500:506	arg1	extraction					448:457	The dispersive micro-solid phase extraction	415:457	The dispersive micro-solid phase extraction (D-μ-SPE) of RA from the medical extract samples	415:506	The dispersive micro-solid phase extraction (D-μ-SPE) of RA from the medical extract samples was investigated by using the prepared biocompatible composite as a solid phase.
32194117	2	15	from	samples	500:506	arg1	D-μ-SPE					460:466	D-μ-SPE	460:466	D-μ-SPE	460:466	The dispersive micro-solid phase extraction (D-μ-SPE) of RA from the medical extract samples was investigated by using the prepared biocompatible composite as a solid phase.
32194117	4	16	theme	contact	708:714	arg1	time					716:719	contact time	708:719	contact time	708:719	The important variables (pH, biocompatible composite mass, contact time, and volume of eluent) associated with the extraction process were analyzed by the application of central composite design (CCD).
32194117	4	16	theme	contact	708:714	arg1	pH					674:675	pH	674:675	pH	674:675	The important variables (pH, biocompatible composite mass, contact time, and volume of eluent) associated with the extraction process were analyzed by the application of central composite design (CCD).
32194117	0	17	theme	medical	175:181	arg1	plants					183:188	medical plants	175:188	medical plants	175:188	Biocompatible chitosan-zinc oxide nanocomposite based dispersive micro-solid phase extraction coupled with HPLC-UV for the determination of rosmarinic acid in the extracts of medical plants and water sample.
32194117	5	18	theme	mentioned	887:895	arg1	variables					897:905	the mentioned variables	883:905	the mentioned variables	883:905	The achieved optimum values for the mentioned variables were 7.0, 10 mg, 4 min, and 180 μL, respectively.
32194117	6	19	theme	extraction	961:970	arg1	%					987:987	99.68%	982:987	99.68%	982:987	The extraction recovery (99.68%) obtained from the predicted model was in agreement with the experimental data (98.22 ± 1.33%).
32194117	6	19	theme	extraction	961:970	arg1	recovery					972:979	The extraction recovery	957:979	The extraction recovery (99.68%) obtained from the predicted model	957:1022	The extraction recovery (99.68%) obtained from the predicted model was in agreement with the experimental data (98.22 ± 1.33%).
32194117	2	20	theme	extract	492:498	arg1	samples					500:506	the medical extract samples	480:506	the medical extract samples	480:506	The dispersive micro-solid phase extraction (D-μ-SPE) of RA from the medical extract samples was investigated by using the prepared biocompatible composite as a solid phase.
32194117	1	21	theme	extract	313:319	arg1	samples					321:327	medical extract samples	305:327	medical extract samples using chitosan‑zinc oxide nanoparticles as a biocompatible nanocomposite (CS-ZnO-NC)	305:412	In the present research, a procedure was described for the recovery of rosmarinic acid (RA) from medical extract samples using chitosan‑zinc oxide nanoparticles as a biocompatible nanocomposite (CS-ZnO-NC).
32194117	10	22	theme	recovery	1693:1700	arg1	values					1702:1707	the recovery values	1689:1707	the recovery values of the analyte in the range of 96.6%-105.4% and the precision with relative standard deviation <5.7%	1689:1808	Therefore, the D-μ-SPE-HPLC-UV method could be used for analyzing RA in the samples of the extracts obtained from the medical plants and water with the recovery values of the analyte in the range of 96.6%-105.4% and the precision with relative standard deviation <5.7%.
32194117	4	23	theme	biocompatible	678:690	arg1	mass					702:705	biocompatible composite mass	678:705	biocompatible composite mass	678:705	The important variables (pH, biocompatible composite mass, contact time, and volume of eluent) associated with the extraction process were analyzed by the application of central composite design (CCD).
32194117	4	23	theme	biocompatible	678:690	arg1	pH					674:675	pH	674:675	pH	674:675	The important variables (pH, biocompatible composite mass, contact time, and volume of eluent) associated with the extraction process were analyzed by the application of central composite design (CCD).
32194117	5	24	theme	optimum	864:870	arg1	7.0					912:914	7.0	912:914	7.0	912:914	The achieved optimum values for the mentioned variables were 7.0, 10 mg, 4 min, and 180 μL, respectively.
32194117	5	24	theme	optimum	864:870	arg1	values					872:877	The achieved optimum values	851:877	The achieved optimum values for the mentioned variables	851:905	The achieved optimum values for the mentioned variables were 7.0, 10 mg, 4 min, and 180 μL, respectively.
32194117	4	25	theme	eluent	736:741	arg1	mass					702:705	biocompatible composite mass	678:705	biocompatible composite mass	678:705	The important variables (pH, biocompatible composite mass, contact time, and volume of eluent) associated with the extraction process were analyzed by the application of central composite design (CCD).
32194117	4	25	theme	eluent	736:741	arg1	time					716:719	contact time	708:719	contact time	708:719	The important variables (pH, biocompatible composite mass, contact time, and volume of eluent) associated with the extraction process were analyzed by the application of central composite design (CCD).
32194117	4	25	theme	eluent	736:741	arg1	volume					726:731	volume	726:731	volume	726:731	The important variables (pH, biocompatible composite mass, contact time, and volume of eluent) associated with the extraction process were analyzed by the application of central composite design (CCD).
32194117	4	25	theme	eluent	736:741	arg1	pH					674:675	pH	674:675	pH	674:675	The important variables (pH, biocompatible composite mass, contact time, and volume of eluent) associated with the extraction process were analyzed by the application of central composite design (CCD).
32194117	7	26	theme	optimum	1117:1123	arg1	conditions					1125:1134	the obtained optimum conditions	1104:1134	the obtained optimum conditions	1104:1134	In addition, under the obtained optimum conditions and over the concentration in the range of 2-3500 ng mL-1, a linear calibration curve was obtained with R2 > 0.993.
32194117	1	27	theme	oxide	349:353	arg1	nanoparticles					355:367	chitosan‑zinc oxide nanoparticles	335:367	chitosan‑zinc oxide nanoparticles	335:367	In the present research, a procedure was described for the recovery of rosmarinic acid (RA) from medical extract samples using chitosan‑zinc oxide nanoparticles as a biocompatible nanocomposite (CS-ZnO-NC).
32194117	1	27	theme	oxide	349:353	arg1	nanocomposite					388:400	a biocompatible nanocomposite	372:400	a biocompatible nanocomposite (CS-ZnO-NC)	372:412	In the present research, a procedure was described for the recovery of rosmarinic acid (RA) from medical extract samples using chitosan‑zinc oxide nanoparticles as a biocompatible nanocomposite (CS-ZnO-NC).
32194117	8	28	theme	detection	1265:1273	arg1	values					1306:1311	detection (LOD) and quantification (LOQ) values	1265:1311	detection (LOD) and quantification (LOQ) values	1265:1311	The limit of detection (LOD) and quantification (LOQ) values were computed, and the obtained ranges were respectively from 0.060 to 0.089 ng mL-1 and 0.201 to 0.297 ng mL-1.
32194117	9	29	theme	enrichment	1443:1452	arg1	factors					1454:1460	the enrichment factors	1439:1460	the enrichment factors	1439:1460	In addition, the enrichment factors were obtained in the range of 93.7-110.5 with preconcentration factor of 83.3.
32194117	10	30	with	%	1751:1751	arg1	deviation					1794:1802	relative standard deviation	1776:1802	relative standard deviation	1776:1802	Therefore, the D-μ-SPE-HPLC-UV method could be used for analyzing RA in the samples of the extracts obtained from the medical plants and water with the recovery values of the analyte in the range of 96.6%-105.4% and the precision with relative standard deviation <5.7%.
32194117	0	31	theme	Biocompatible	0:12	arg1	nanocomposite					34:46	Biocompatible chitosan-zinc oxide nanocomposite	0:46	Biocompatible chitosan-zinc oxide nanocomposite	0:46	Biocompatible chitosan-zinc oxide nanocomposite based dispersive micro-solid phase extraction coupled with HPLC-UV for the determination of rosmarinic acid in the extracts of medical plants and water sample.
32194117	10	32	from	values	1702:1707	arg1	range					1731:1735	the range	1727:1735	the range of 96.6%-105.4% and the precision with relative standard deviation <5.7%	1727:1808	Therefore, the D-μ-SPE-HPLC-UV method could be used for analyzing RA in the samples of the extracts obtained from the medical plants and water with the recovery values of the analyte in the range of 96.6%-105.4% and the precision with relative standard deviation <5.7%.
32194117	2	33	theme	phase	442:446	arg1	extraction					448:457	The dispersive micro-solid phase extraction	415:457	The dispersive micro-solid phase extraction (D-μ-SPE) of RA from the medical extract samples	415:506	The dispersive micro-solid phase extraction (D-μ-SPE) of RA from the medical extract samples was investigated by using the prepared biocompatible composite as a solid phase.
32194117	2	33	theme	phase	442:446	arg1	D-μ-SPE					460:466	D-μ-SPE	460:466	D-μ-SPE	460:466	The dispersive micro-solid phase extraction (D-μ-SPE) of RA from the medical extract samples was investigated by using the prepared biocompatible composite as a solid phase.
32194117	2	34	theme	dispersive	419:428	arg1	extraction					448:457	The dispersive micro-solid phase extraction	415:457	The dispersive micro-solid phase extraction (D-μ-SPE) of RA from the medical extract samples	415:506	The dispersive micro-solid phase extraction (D-μ-SPE) of RA from the medical extract samples was investigated by using the prepared biocompatible composite as a solid phase.
32194117	2	34	theme	dispersive	419:428	arg1	D-μ-SPE					460:466	D-μ-SPE	460:466	D-μ-SPE	460:466	The dispersive micro-solid phase extraction (D-μ-SPE) of RA from the medical extract samples was investigated by using the prepared biocompatible composite as a solid phase.
32194117	0	35	theme	oxide	28:32	arg1	nanocomposite					34:46	Biocompatible chitosan-zinc oxide nanocomposite	0:46	Biocompatible chitosan-zinc oxide nanocomposite	0:46	Biocompatible chitosan-zinc oxide nanocomposite based dispersive micro-solid phase extraction coupled with HPLC-UV for the determination of rosmarinic acid in the extracts of medical plants and water sample.
32194117	1	36	theme	acid	290:293	arg1	recovery					267:274	the recovery	263:274	the recovery of rosmarinic acid (RA) from medical extract samples using chitosan‑zinc oxide nanoparticles as a biocompatible nanocomposite (CS-ZnO-NC)	263:412	In the present research, a procedure was described for the recovery of rosmarinic acid (RA) from medical extract samples using chitosan‑zinc oxide nanoparticles as a biocompatible nanocomposite (CS-ZnO-NC).
32194117	2	37	theme	RA	472:473	arg1	extraction					448:457	The dispersive micro-solid phase extraction	415:457	The dispersive micro-solid phase extraction (D-μ-SPE) of RA from the medical extract samples	415:506	The dispersive micro-solid phase extraction (D-μ-SPE) of RA from the medical extract samples was investigated by using the prepared biocompatible composite as a solid phase.
32194117	2	37	theme	RA	472:473	arg1	D-μ-SPE					460:466	D-μ-SPE	460:466	D-μ-SPE	460:466	The dispersive micro-solid phase extraction (D-μ-SPE) of RA from the medical extract samples was investigated by using the prepared biocompatible composite as a solid phase.
32194117	10	38	theme	D-μ-SPE-HPLC-UV	1556:1570	arg1	method					1572:1577	the D-μ-SPE-HPLC-UV method	1552:1577	the D-μ-SPE-HPLC-UV method	1552:1577	Therefore, the D-μ-SPE-HPLC-UV method could be used for analyzing RA in the samples of the extracts obtained from the medical plants and water with the recovery values of the analyte in the range of 96.6%-105.4% and the precision with relative standard deviation <5.7%.
32194117	7	39	theme	calibration	1204:1214	arg1	curve					1216:1220	a linear calibration curve	1195:1220	a linear calibration curve	1195:1220	In addition, under the obtained optimum conditions and over the concentration in the range of 2-3500 ng mL-1, a linear calibration curve was obtained with R2 > 0.993.
32194117	10	40	dep	%	1751:1751	arg1	%					1808:1808	<5.7%	1804:1808	<5.7%	1804:1808	Therefore, the D-μ-SPE-HPLC-UV method could be used for analyzing RA in the samples of the extracts obtained from the medical plants and water with the recovery values of the analyte in the range of 96.6%-105.4% and the precision with relative standard deviation <5.7%.
32194117	10	41	theme	analyte	1716:1722	arg1	values					1702:1707	the recovery values	1689:1707	the recovery values of the analyte in the range of 96.6%-105.4% and the precision with relative standard deviation <5.7%	1689:1808	Therefore, the D-μ-SPE-HPLC-UV method could be used for analyzing RA in the samples of the extracts obtained from the medical plants and water with the recovery values of the analyte in the range of 96.6%-105.4% and the precision with relative standard deviation <5.7%.
32194117	1	42	from	samples	321:327	arg1	recovery					267:274	the recovery	263:274	the recovery of rosmarinic acid (RA) from medical extract samples using chitosan‑zinc oxide nanoparticles as a biocompatible nanocomposite (CS-ZnO-NC)	263:412	In the present research, a procedure was described for the recovery of rosmarinic acid (RA) from medical extract samples using chitosan‑zinc oxide nanoparticles as a biocompatible nanocomposite (CS-ZnO-NC).
32194117	9	43	theme	93.7-110.5	1492:1501	arg1	range					1483:1487	the range	1479:1487	the range of 93.7-110.5 with preconcentration factor of 83.3	1479:1538	In addition, the enrichment factors were obtained in the range of 93.7-110.5 with preconcentration factor of 83.3.
32194117	6	44	theme	experimental	1050:1061	arg1	%					1081:1081	98.22 ± 1.33%	1069:1081	98.22 ± 1.33%	1069:1081	The extraction recovery (99.68%) obtained from the predicted model was in agreement with the experimental data (98.22 ± 1.33%).
32194117	6	44	theme	experimental	1050:1061	arg1	data					1063:1066	the experimental data	1046:1066	the experimental data (98.22 ± 1.33%)	1046:1082	The extraction recovery (99.68%) obtained from the predicted model was in agreement with the experimental data (98.22 ± 1.33%).
32194117	9	45	theme	preconcentration	1508:1523	arg1	factor					1525:1530	preconcentration factor	1508:1530	preconcentration factor of 83.3	1508:1538	In addition, the enrichment factors were obtained in the range of 93.7-110.5 with preconcentration factor of 83.3.
32194117	7	46	theme	2-3500 ng mL-1	1179:1192	arg1	range					1170:1174	the range	1166:1174	the range of 2-3500 ng mL-1	1166:1192	In addition, under the obtained optimum conditions and over the concentration in the range of 2-3500 ng mL-1, a linear calibration curve was obtained with R2 > 0.993.
32194117	10	47	used	used	1588:1591	arg2	method					1572:1577	the D-μ-SPE-HPLC-UV method	1552:1577	the D-μ-SPE-HPLC-UV method	1552:1577	Therefore, the D-μ-SPE-HPLC-UV method could be used for analyzing RA in the samples of the extracts obtained from the medical plants and water with the recovery values of the analyte in the range of 96.6%-105.4% and the precision with relative standard deviation <5.7%.
32194117	8	48	dep	0.297 ng mL-1	1411:1423	arg1	to					1408:1409	to	1408:1409	to	1408:1409	The limit of detection (LOD) and quantification (LOQ) values were computed, and the obtained ranges were respectively from 0.060 to 0.089 ng mL-1 and 0.201 to 0.297 ng mL-1.
32194117	10	49	from	analyte	1716:1722	arg1	range					1731:1735	the range	1727:1735	the range of 96.6%-105.4% and the precision with relative standard deviation <5.7%	1727:1808	Therefore, the D-μ-SPE-HPLC-UV method could be used for analyzing RA in the samples of the extracts obtained from the medical plants and water with the recovery values of the analyte in the range of 96.6%-105.4% and the precision with relative standard deviation <5.7%.
32194117	0	50	theme	water	194:198	arg1	sample					200:205	water sample	194:205	water sample	194:205	Biocompatible chitosan-zinc oxide nanocomposite based dispersive micro-solid phase extraction coupled with HPLC-UV for the determination of rosmarinic acid in the extracts of medical plants and water sample.
32194117	4	51	theme	design	837:842	arg1	application					804:814	the application	800:814	the application of central composite design (CCD)	800:848	The important variables (pH, biocompatible composite mass, contact time, and volume of eluent) associated with the extraction process were analyzed by the application of central composite design (CCD).
32194117	7	52	from	concentration	1149:1161	arg1	range					1170:1174	the range	1166:1174	the range of 2-3500 ng mL-1	1166:1192	In addition, under the obtained optimum conditions and over the concentration in the range of 2-3500 ng mL-1, a linear calibration curve was obtained with R2 > 0.993.
32194117	10	53	theme	%	1751:1751	arg1	range					1731:1735	the range	1727:1735	the range of 96.6%-105.4% and the precision with relative standard deviation <5.7%	1727:1808	Therefore, the D-μ-SPE-HPLC-UV method could be used for analyzing RA in the samples of the extracts obtained from the medical plants and water with the recovery values of the analyte in the range of 96.6%-105.4% and the precision with relative standard deviation <5.7%.
32194117	8	54	dep	0.089 ng mL-1	1384:1396	arg1	to					1381:1382	to	1381:1382	to	1381:1382	The limit of detection (LOD) and quantification (LOQ) values were computed, and the obtained ranges were respectively from 0.060 to 0.089 ng mL-1 and 0.201 to 0.297 ng mL-1.
32194117	0	55	from	extracts	163:170	arg1	determination					123:135	the determination	119:135	the determination of rosmarinic acid in the extracts of medical plants and water sample	119:205	Biocompatible chitosan-zinc oxide nanocomposite based dispersive micro-solid phase extraction coupled with HPLC-UV for the determination of rosmarinic acid in the extracts of medical plants and water sample.
32194117	10	56	theme	standard	1785:1792	arg1	deviation					1794:1802	relative standard deviation	1776:1802	relative standard deviation	1776:1802	Therefore, the D-μ-SPE-HPLC-UV method could be used for analyzing RA in the samples of the extracts obtained from the medical plants and water with the recovery values of the analyte in the range of 96.6%-105.4% and the precision with relative standard deviation <5.7%.
32194117	0	57	theme	micro-solid	65:75	arg1	extraction					83:92	dispersive micro-solid phase extraction	54:92	dispersive micro-solid phase extraction coupled with HPLC-UV for the determination of rosmarinic acid in the extracts of medical plants and water sample	54:205	Biocompatible chitosan-zinc oxide nanocomposite based dispersive micro-solid phase extraction coupled with HPLC-UV for the determination of rosmarinic acid in the extracts of medical plants and water sample.
32194117	6	58	with	agreement	1031:1039	arg1	%					1081:1081	98.22 ± 1.33%	1069:1081	98.22 ± 1.33%	1069:1081	The extraction recovery (99.68%) obtained from the predicted model was in agreement with the experimental data (98.22 ± 1.33%).
32194117	6	58	with	agreement	1031:1039	arg1	data					1063:1066	the experimental data	1046:1066	the experimental data (98.22 ± 1.33%)	1046:1082	The extraction recovery (99.68%) obtained from the predicted model was in agreement with the experimental data (98.22 ± 1.33%).
32194117	4	59	theme	central	819:825	arg1	CCD					845:847	CCD	845:847	CCD	845:847	The important variables (pH, biocompatible composite mass, contact time, and volume of eluent) associated with the extraction process were analyzed by the application of central composite design (CCD).
32194117	4	59	theme	central	819:825	arg1	design					837:842	central composite design	819:842	central composite design (CCD)	819:848	The important variables (pH, biocompatible composite mass, contact time, and volume of eluent) associated with the extraction process were analyzed by the application of central composite design (CCD).
32194117	4	60	theme	important	653:661	arg1	variables					663:671	The important variables	649:671	The important variables (pH, biocompatible composite mass, contact time, and volume of eluent) associated with the extraction process	649:781	The important variables (pH, biocompatible composite mass, contact time, and volume of eluent) associated with the extraction process were analyzed by the application of central composite design (CCD).
32194117	0	61	theme	acid	151:154	arg1	determination					123:135	the determination	119:135	the determination of rosmarinic acid in the extracts of medical plants and water sample	119:205	Biocompatible chitosan-zinc oxide nanocomposite based dispersive micro-solid phase extraction coupled with HPLC-UV for the determination of rosmarinic acid in the extracts of medical plants and water sample.
32194117	2	62	theme	prepared	538:545	arg1	phase					582:586	a solid phase	574:586	a solid phase	574:586	The dispersive micro-solid phase extraction (D-μ-SPE) of RA from the medical extract samples was investigated by using the prepared biocompatible composite as a solid phase.
32194117	2	62	theme	prepared	538:545	arg1	composite					561:569	the prepared biocompatible composite	534:569	the prepared biocompatible composite	534:569	The dispersive micro-solid phase extraction (D-μ-SPE) of RA from the medical extract samples was investigated by using the prepared biocompatible composite as a solid phase.
32194117	1	63	theme	present	215:221	arg1	research					223:230	the present research	211:230	the present research	211:230	In the present research, a procedure was described for the recovery of rosmarinic acid (RA) from medical extract samples using chitosan‑zinc oxide nanoparticles as a biocompatible nanocomposite (CS-ZnO-NC).
32194117	4	64	theme	composite	692:700	arg1	mass					702:705	biocompatible composite mass	678:705	biocompatible composite mass	678:705	The important variables (pH, biocompatible composite mass, contact time, and volume of eluent) associated with the extraction process were analyzed by the application of central composite design (CCD).
32194117	4	64	theme	composite	692:700	arg1	pH					674:675	pH	674:675	pH	674:675	The important variables (pH, biocompatible composite mass, contact time, and volume of eluent) associated with the extraction process were analyzed by the application of central composite design (CCD).
32194117	0	65	theme	plants	183:188	arg1	extracts					163:170	the extracts	159:170	the extracts of medical plants and water sample	159:205	Biocompatible chitosan-zinc oxide nanocomposite based dispersive micro-solid phase extraction coupled with HPLC-UV for the determination of rosmarinic acid in the extracts of medical plants and water sample.
32194117	2	66	theme	medical	484:490	arg1	samples					500:506	the medical extract samples	480:506	the medical extract samples	480:506	The dispersive micro-solid phase extraction (D-μ-SPE) of RA from the medical extract samples was investigated by using the prepared biocompatible composite as a solid phase.
32194117	5	67	theme	achieved	855:862	arg1	7.0					912:914	7.0	912:914	7.0	912:914	The achieved optimum values for the mentioned variables were 7.0, 10 mg, 4 min, and 180 μL, respectively.
32194117	5	67	theme	achieved	855:862	arg1	values					872:877	The achieved optimum values	851:877	The achieved optimum values for the mentioned variables	851:905	The achieved optimum values for the mentioned variables were 7.0, 10 mg, 4 min, and 180 μL, respectively.
32194117	3	68	used	used	612:615	arg2	method					601:606	The HPLC-UV method	589:606	The HPLC-UV method	589:606	The HPLC-UV method was used for measuring the extracted RA.
32194117	7	69	theme	obtained	1108:1115	arg1	conditions					1125:1134	the obtained optimum conditions	1104:1134	the obtained optimum conditions	1104:1134	In addition, under the obtained optimum conditions and over the concentration in the range of 2-3500 ng mL-1, a linear calibration curve was obtained with R2 > 0.993.
32194117	1	70	theme	chitosan‑zinc	335:347	arg1	nanoparticles					355:367	chitosan‑zinc oxide nanoparticles	335:367	chitosan‑zinc oxide nanoparticles	335:367	In the present research, a procedure was described for the recovery of rosmarinic acid (RA) from medical extract samples using chitosan‑zinc oxide nanoparticles as a biocompatible nanocomposite (CS-ZnO-NC).
32194117	1	70	theme	chitosan‑zinc	335:347	arg1	nanocomposite					388:400	a biocompatible nanocomposite	372:400	a biocompatible nanocomposite (CS-ZnO-NC)	372:412	In the present research, a procedure was described for the recovery of rosmarinic acid (RA) from medical extract samples using chitosan‑zinc oxide nanoparticles as a biocompatible nanocomposite (CS-ZnO-NC).
32194117	6	71	theme	predicted	1008:1016	arg1	model					1018:1022	the predicted model	1004:1022	the predicted model	1004:1022	The extraction recovery (99.68%) obtained from the predicted model was in agreement with the experimental data (98.22 ± 1.33%).
32194117	0	72	theme	chitosan-zinc	14:26	arg1	nanocomposite					34:46	Biocompatible chitosan-zinc oxide nanocomposite	0:46	Biocompatible chitosan-zinc oxide nanocomposite	0:46	Biocompatible chitosan-zinc oxide nanocomposite based dispersive micro-solid phase extraction coupled with HPLC-UV for the determination of rosmarinic acid in the extracts of medical plants and water sample.
32194117	10	73	with	precision	1761:1769	arg1	deviation					1794:1802	relative standard deviation	1776:1802	relative standard deviation	1776:1802	Therefore, the D-μ-SPE-HPLC-UV method could be used for analyzing RA in the samples of the extracts obtained from the medical plants and water with the recovery values of the analyte in the range of 96.6%-105.4% and the precision with relative standard deviation <5.7%.
32194117	1	74	theme	rosmarinic	279:288	arg1	acid					290:293	rosmarinic acid	279:293	rosmarinic acid (RA)	279:298	In the present research, a procedure was described for the recovery of rosmarinic acid (RA) from medical extract samples using chitosan‑zinc oxide nanoparticles as a biocompatible nanocomposite (CS-ZnO-NC).
32194117	1	74	theme	rosmarinic	279:288	arg1	RA					296:297	RA	296:297	RA	296:297	In the present research, a procedure was described for the recovery of rosmarinic acid (RA) from medical extract samples using chitosan‑zinc oxide nanoparticles as a biocompatible nanocomposite (CS-ZnO-NC).
32194117	2	75	theme	micro-solid	430:440	arg1	extraction					448:457	The dispersive micro-solid phase extraction	415:457	The dispersive micro-solid phase extraction (D-μ-SPE) of RA from the medical extract samples	415:506	The dispersive micro-solid phase extraction (D-μ-SPE) of RA from the medical extract samples was investigated by using the prepared biocompatible composite as a solid phase.
32194117	2	75	theme	micro-solid	430:440	arg1	D-μ-SPE					460:466	D-μ-SPE	460:466	D-μ-SPE	460:466	The dispersive micro-solid phase extraction (D-μ-SPE) of RA from the medical extract samples was investigated by using the prepared biocompatible composite as a solid phase.
32194117	3	76	theme	extracted	635:643	arg1	RA					645:646	the extracted RA	631:646	the extracted RA	631:646	The HPLC-UV method was used for measuring the extracted RA.
32194117	0	77	theme	dispersive	54:63	arg1	extraction					83:92	dispersive micro-solid phase extraction	54:92	dispersive micro-solid phase extraction coupled with HPLC-UV for the determination of rosmarinic acid in the extracts of medical plants and water sample	54:205	Biocompatible chitosan-zinc oxide nanocomposite based dispersive micro-solid phase extraction coupled with HPLC-UV for the determination of rosmarinic acid in the extracts of medical plants and water sample.
32194117	7	78	theme	linear	1197:1202	arg1	curve					1216:1220	a linear calibration curve	1195:1220	a linear calibration curve	1195:1220	In addition, under the obtained optimum conditions and over the concentration in the range of 2-3500 ng mL-1, a linear calibration curve was obtained with R2 > 0.993.
32194117	8	79	theme	quantification	1285:1298	arg1	values					1306:1311	detection (LOD) and quantification (LOQ) values	1265:1311	detection (LOD) and quantification (LOQ) values	1265:1311	The limit of detection (LOD) and quantification (LOQ) values were computed, and the obtained ranges were respectively from 0.060 to 0.089 ng mL-1 and 0.201 to 0.297 ng mL-1.
32194117	0	80	theme	phase	77:81	arg1	extraction					83:92	dispersive micro-solid phase extraction	54:92	dispersive micro-solid phase extraction coupled with HPLC-UV for the determination of rosmarinic acid in the extracts of medical plants and water sample	54:205	Biocompatible chitosan-zinc oxide nanocomposite based dispersive micro-solid phase extraction coupled with HPLC-UV for the determination of rosmarinic acid in the extracts of medical plants and water sample.
32194117	10	81	theme	-105.4	1745:1750	arg1	%					1744:1744	%	1744:1744	%	1744:1744	Therefore, the D-μ-SPE-HPLC-UV method could be used for analyzing RA in the samples of the extracts obtained from the medical plants and water with the recovery values of the analyte in the range of 96.6%-105.4% and the precision with relative standard deviation <5.7%.
32194117	8	82	theme	LOQ	1301:1303	arg1	values					1306:1311	detection (LOD) and quantification (LOQ) values	1265:1311	detection (LOD) and quantification (LOQ) values	1265:1311	The limit of detection (LOD) and quantification (LOQ) values were computed, and the obtained ranges were respectively from 0.060 to 0.089 ng mL-1 and 0.201 to 0.297 ng mL-1.
32194117	1	83	theme	medical	305:311	arg1	samples					321:327	medical extract samples	305:327	medical extract samples using chitosan‑zinc oxide nanoparticles as a biocompatible nanocomposite (CS-ZnO-NC)	305:412	In the present research, a procedure was described for the recovery of rosmarinic acid (RA) from medical extract samples using chitosan‑zinc oxide nanoparticles as a biocompatible nanocomposite (CS-ZnO-NC).
32194117	4	84	dep	variables	663:671	arg1	mass					702:705	biocompatible composite mass	678:705	biocompatible composite mass	678:705	The important variables (pH, biocompatible composite mass, contact time, and volume of eluent) associated with the extraction process were analyzed by the application of central composite design (CCD).
32194117	4	84	dep	variables	663:671	arg1	time					716:719	contact time	708:719	contact time	708:719	The important variables (pH, biocompatible composite mass, contact time, and volume of eluent) associated with the extraction process were analyzed by the application of central composite design (CCD).
32194117	4	84	dep	variables	663:671	arg1	volume					726:731	volume	726:731	volume	726:731	The important variables (pH, biocompatible composite mass, contact time, and volume of eluent) associated with the extraction process were analyzed by the application of central composite design (CCD).
32194117	4	84	dep	variables	663:671	arg1	pH					674:675	pH	674:675	pH	674:675	The important variables (pH, biocompatible composite mass, contact time, and volume of eluent) associated with the extraction process were analyzed by the application of central composite design (CCD).
32194117	10	85	theme	96.6	1740:1743	arg1	%					1744:1744	%	1744:1744	%	1744:1744	Therefore, the D-μ-SPE-HPLC-UV method could be used for analyzing RA in the samples of the extracts obtained from the medical plants and water with the recovery values of the analyte in the range of 96.6%-105.4% and the precision with relative standard deviation <5.7%.
32194117	8	86	theme	values	1306:1311	arg1	limit					1256:1260	The limit	1252:1260	The limit of detection (LOD) and quantification (LOQ) values	1252:1311	The limit of detection (LOD) and quantification (LOQ) values were computed, and the obtained ranges were respectively from 0.060 to 0.089 ng mL-1 and 0.201 to 0.297 ng mL-1.
32194117	0	87	from	acid	151:154	arg1	extracts					163:170	the extracts	159:170	the extracts of medical plants and water sample	159:205	Biocompatible chitosan-zinc oxide nanocomposite based dispersive micro-solid phase extraction coupled with HPLC-UV for the determination of rosmarinic acid in the extracts of medical plants and water sample.
32194117	10	88	theme	precision	1761:1769	arg1	range					1731:1735	the range	1727:1735	the range of 96.6%-105.4% and the precision with relative standard deviation <5.7%	1727:1808	Therefore, the D-μ-SPE-HPLC-UV method could be used for analyzing RA in the samples of the extracts obtained from the medical plants and water with the recovery values of the analyte in the range of 96.6%-105.4% and the precision with relative standard deviation <5.7%.
32194117	4	89	theme	extraction	764:773	arg1	process					775:781	the extraction process	760:781	the extraction process	760:781	The important variables (pH, biocompatible composite mass, contact time, and volume of eluent) associated with the extraction process were analyzed by the application of central composite design (CCD).
34536479	7	0	theme	release	1074:1080	arg1	%					1081:1081	equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release%	999:1081	equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response	999:1101	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	2	1	theme	N'N-methylene	329:341	arg1	crosslinker					378:388	a crosslinker	376:388	a crosslinker	376:388	N'N-methylene bis acrylamide (MBA) was used as a crosslinker.
34536479	2	1	theme	N'N-methylene	329:341	arg1	MBA					359:361	MBA	359:361	MBA	359:361	N'N-methylene bis acrylamide (MBA) was used as a crosslinker.
34536479	2	1	theme	N'N-methylene	329:341	arg1	acrylamide					347:356	N'N-methylene bis acrylamide	329:356	N'N-methylene bis acrylamide (MBA)	329:362	N'N-methylene bis acrylamide (MBA) was used as a crosslinker.
34536479	7	2	from	%	1081:1081	arg1	16 h					1086:1089	16 h	1086:1089	16 h as response	1086:1101	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	8	3	theme	solution	1367:1374	arg1	pH					1376:1377	a solution pH	1365:1377	a solution pH of 7.4	1365:1384	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	6	4	theme	diltiazem	804:812	arg1	DT					829:830	DT	829:830	DT	829:830	The release kinetics of a model drug diltiazem hydrochloride (DT) was studied with these hydrogels.
34536479	6	4	theme	diltiazem	804:812	arg1	hydrochloride					814:826	a model drug diltiazem hydrochloride	791:826	a model drug diltiazem hydrochloride (DT)	791:831	The release kinetics of a model drug diltiazem hydrochloride (DT) was studied with these hydrogels.
34536479	7	5	theme	surface	968:974	arg1	RSM					989:991	RSM	989:991	RSM	989:991	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	7	5	theme	surface	968:974	arg1	methodology					976:986	response surface methodology	959:986	response surface methodology (RSM)	959:992	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	1	6	theme	copolymer	181:189	arg1	hydrogels					191:199	Pectin grafted polyacrylic copolymer hydrogels	154:199	Pectin grafted polyacrylic copolymer hydrogels	154:199	Pectin grafted polyacrylic copolymer hydrogels were made by free radical crosslink copolymerization of acrylic acid (AA) and acrylamide (AM) in an aqueous solution of pectin.
34536479	6	7	theme	model	793:797	arg1	DT					829:830	DT	829:830	DT	829:830	The release kinetics of a model drug diltiazem hydrochloride (DT) was studied with these hydrogels.
34536479	6	7	theme	model	793:797	arg1	hydrochloride					814:826	a model drug diltiazem hydrochloride	791:826	a model drug diltiazem hydrochloride (DT)	791:831	The release kinetics of a model drug diltiazem hydrochloride (DT) was studied with these hydrogels.
34536479	4	8	theme	pectin	588:593	arg1	pectin					588:593	pectin	588:593	pectin	588:593	Several filled hydrogels were prepared by varying the amount of pectin and APG filler.
34536479	4	8	theme	pectin	588:593	arg1	filler					603:608	APG filler	599:608	APG filler	599:608	Several filled hydrogels were prepared by varying the amount of pectin and APG filler.
34536479	4	8	theme	pectin	588:593	arg1	amount					578:583	the amount	574:583	the amount of pectin and APG filler	574:608	Several filled hydrogels were prepared by varying the amount of pectin and APG filler.
34536479	5	9	theme	13C	655:657	arg1	NMR					659:661	13C NMR	655:661	13C NMR	655:661	These hydrogels were characterized by FTIR, 13C NMR, XRD, TGA, SEM, mechanical properties, DMA, swelling, diffusion characteristics and network parameters.
34536479	3	10	theme	gel	519:521	arg1	network					494:500	the network	490:500	the network of the copolymer gel	490:521	During the polymerization reaction the attapulgite (APG) filler was also incorporated in situ into the network of the copolymer gel.
34536479	8	11	theme	concentration	1226:1238	arg1	%					1198:1198	1%	1197:1198	1% each of initiator and MBA concentration	1197:1238	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	11	theme	concentration	1226:1238	arg1	concentration					1226:1238	MBA concentration	1222:1238	MBA concentration	1222:1238	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	11	theme	concentration	1226:1238	arg1	AA					1148:1149	5:1 AA	1144:1149	5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG	1144:1267	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	11	theme	concentration	1226:1238	arg1	APG					1265:1267	2 wt% APG	1259:1267	2 wt% APG	1259:1267	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	11	theme	concentration	1226:1238	arg1	concentration					1182:1194	25 wt% monomer concentration	1167:1194	25 wt% monomer concentration	1167:1194	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	11	theme	concentration	1226:1238	arg1	initiator					1208:1216	initiator	1208:1216	initiator	1208:1216	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	11	theme	concentration	1226:1238	arg1	pectin					1248:1253	18 wt% pectin	1241:1253	18 wt% pectin	1241:1253	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	11	theme	concentration	1226:1238	arg1	ratio					1160:1164	AM molar ratio	1151:1164	AM molar ratio	1151:1164	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	4	12	theme	APG	599:601	arg1	filler					603:608	APG filler	599:608	APG filler	599:608	Several filled hydrogels were prepared by varying the amount of pectin and APG filler.
34536479	0	13	theme	polyacrylic	127:137	arg1	gel					149:151	polyacrylic copolymer gel	127:151	polyacrylic copolymer gel	127:151	Effect of pectin and attapulgite filler on swelling, network parameters and controlled release of diltiazem hydrochloride from polyacrylic copolymer gel.
34536479	1	14	theme	radical	219:225	arg1	copolymerization					237:252	free radical crosslink copolymerization	214:252	free radical crosslink copolymerization of acrylic acid (AA) and acrylamide (AM)	214:293	Pectin grafted polyacrylic copolymer hydrogels were made by free radical crosslink copolymerization of acrylic acid (AA) and acrylamide (AM) in an aqueous solution of pectin.
34536479	8	15	theme	18 wt	1241:1245	arg1	AA					1148:1149	5:1 AA	1144:1149	5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG	1144:1267	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	15	theme	18 wt	1241:1245	arg1	pectin					1248:1253	18 wt% pectin	1241:1253	18 wt% pectin	1241:1253	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	7	16	theme	composite	927:935	arg1	model					950:954	a central composite design (CCD) model	917:954	a central composite design (CCD) model of response surface methodology (RSM)	917:992	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	8	17	theme	2 wt	1259:1262	arg1	APG					1265:1267	2 wt% APG	1259:1267	2 wt% APG	1259:1267	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	17	theme	2 wt	1259:1262	arg1	AA					1148:1149	5:1 AA	1144:1149	5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG	1144:1267	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	7	18	theme	drug	1069:1072	arg1	release					1074:1080	drug release	1069:1080	drug release	1069:1080	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	8	19	dep	%	1198:1198	arg1	each					1200:1203	each	1200:1203	each	1200:1203	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	4	20	theme	Several	524:530	arg1	hydrogels					539:547	Several filled hydrogels	524:547	Several filled hydrogels	524:547	Several filled hydrogels were prepared by varying the amount of pectin and APG filler.
34536479	2	21	used	used	368:371	arg2	MBA					359:361	MBA	359:361	MBA	359:361	N'N-methylene bis acrylamide (MBA) was used as a crosslinker.
34536479	2	21	used	used	368:371	arg2	acrylamide					347:356	N'N-methylene bis acrylamide	329:356	N'N-methylene bis acrylamide (MBA)	329:362	N'N-methylene bis acrylamide (MBA) was used as a crosslinker.
34536479	2	21	used	used	368:371	arg2	crosslinker					378:388	a crosslinker	376:388	a crosslinker	376:388	N'N-methylene bis acrylamide (MBA) was used as a crosslinker.
34536479	1	22	theme	pectin	321:326	arg1	solution					309:316	an aqueous solution	298:316	an aqueous solution of pectin	298:326	Pectin grafted polyacrylic copolymer hydrogels were made by free radical crosslink copolymerization of acrylic acid (AA) and acrylamide (AM) in an aqueous solution of pectin.
34536479	7	23	theme	wt	871:872	arg1	MBA					894:896	MBA	894:896	MBA	894:896	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	7	23	theme	wt	871:872	arg1	APG					886:888	APG	886:888	APG	886:888	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	7	23	theme	wt	871:872	arg1	pectin					878:883	pectin	878:883	pectin	878:883	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	7	23	theme	wt	871:872	arg1	%					873:873	The wt%	867:873	The wt% of pectin, APG and MBA	867:896	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	1	24	theme	grafted	161:167	arg1	hydrogels					191:199	Pectin grafted polyacrylic copolymer hydrogels	154:199	Pectin grafted polyacrylic copolymer hydrogels	154:199	Pectin grafted polyacrylic copolymer hydrogels were made by free radical crosslink copolymerization of acrylic acid (AA) and acrylamide (AM) in an aqueous solution of pectin.
34536479	0	25	from	Effect	0:5	arg1	parameters					61:70	network parameters	53:70	network parameters	53:70	Effect of pectin and attapulgite filler on swelling, network parameters and controlled release of diltiazem hydrochloride from polyacrylic copolymer gel.
34536479	0	25	from	Effect	0:5	arg1	release					87:93	controlled release	76:93	controlled release of diltiazem hydrochloride from polyacrylic copolymer gel	76:151	Effect of pectin and attapulgite filler on swelling, network parameters and controlled release of diltiazem hydrochloride from polyacrylic copolymer gel.
34536479	0	25	from	Effect	0:5	arg1	swelling					43:50	swelling	43:50	swelling	43:50	Effect of pectin and attapulgite filler on swelling, network parameters and controlled release of diltiazem hydrochloride from polyacrylic copolymer gel.
34536479	8	26	theme	molar	1154:1158	arg1	ratio					1160:1164	AM molar ratio	1151:1164	AM molar ratio	1151:1164	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	26	theme	molar	1154:1158	arg1	AA					1148:1149	5:1 AA	1144:1149	5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG	1144:1267	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	0	27	theme	controlled	76:85	arg1	release					87:93	controlled release	76:93	controlled release of diltiazem hydrochloride from polyacrylic copolymer gel	76:151	Effect of pectin and attapulgite filler on swelling, network parameters and controlled release of diltiazem hydrochloride from polyacrylic copolymer gel.
34536479	3	28	theme	polymerization	402:415	arg1	reaction					417:424	the polymerization reaction	398:424	the polymerization reaction	398:424	During the polymerization reaction the attapulgite (APG) filler was also incorporated in situ into the network of the copolymer gel.
34536479	7	29	theme	adsorption	1038:1047	arg1	%					1081:1081	equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release%	999:1081	equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response	999:1101	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	8	30	theme	17.75	1296:1300	arg1	92.5					1349:1352	92.5	1349:1352	92.5	1349:1352	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	30	theme	17.75	1296:1300	arg1	17.75					1296:1300	17.75	1296:1300	17.75	1296:1300	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	30	theme	17.75	1296:1300	arg1	loading					1308:1314	drug loading	1303:1314	drug loading of 27.58	1303:1323	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	30	theme	17.75	1296:1300	arg1	%					1344:1344	a drug release %	1329:1344	a drug release % of 92.5	1329:1352	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	30	theme	17.75	1296:1300	arg1	27.58					1319:1323	27.58	1319:1323	27.58	1319:1323	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	30	theme	17.75	1296:1300	arg1	ESR					1289:1291	an optimized ESR	1276:1291	an optimized ESR of 17.75	1276:1300	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	31	theme	%	1172:1172	arg1	AA					1148:1149	5:1 AA	1144:1149	5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG	1144:1267	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	31	theme	%	1172:1172	arg1	concentration					1182:1194	25 wt% monomer concentration	1167:1194	25 wt% monomer concentration	1167:1194	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	5	32	theme	diffusion	717:725	arg1	characteristics					727:741	diffusion characteristics	717:741	diffusion characteristics	717:741	These hydrogels were characterized by FTIR, 13C NMR, XRD, TGA, SEM, mechanical properties, DMA, swelling, diffusion characteristics and network parameters.
34536479	8	33	theme	drug	1303:1306	arg1	loading					1308:1314	drug loading	1303:1314	drug loading of 27.58	1303:1323	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	0	34	theme	pectin	10:15	arg1	Effect					0:5	Effect	0:5	Effect of pectin and attapulgite filler on swelling, network parameters and controlled release of diltiazem hydrochloride from polyacrylic copolymer gel.	0:152	Effect of pectin and attapulgite filler on swelling, network parameters and controlled release of diltiazem hydrochloride from polyacrylic copolymer gel.
34536479	7	35	theme	ratio	1020:1024	arg1	%					1081:1081	equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release%	999:1081	equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response	999:1101	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	0	36	theme	attapulgite	21:31	arg1	Effect					0:5	Effect	0:5	Effect of pectin and attapulgite filler on swelling, network parameters and controlled release of diltiazem hydrochloride from polyacrylic copolymer gel.	0:152	Effect of pectin and attapulgite filler on swelling, network parameters and controlled release of diltiazem hydrochloride from polyacrylic copolymer gel.
34536479	8	37	dep	AA	1148:1149	arg1	%					1198:1198	1%	1197:1198	1% each of initiator and MBA concentration	1197:1238	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	37	dep	AA	1148:1149	arg1	concentration					1226:1238	MBA concentration	1222:1238	MBA concentration	1222:1238	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	37	dep	AA	1148:1149	arg1	AA					1148:1149	5:1 AA	1144:1149	5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG	1144:1267	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	37	dep	AA	1148:1149	arg1	APG					1265:1267	2 wt% APG	1259:1267	2 wt% APG	1259:1267	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	37	dep	AA	1148:1149	arg1	concentration					1182:1194	25 wt% monomer concentration	1167:1194	25 wt% monomer concentration	1167:1194	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	37	dep	AA	1148:1149	arg1	initiator					1208:1216	initiator	1208:1216	initiator	1208:1216	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	37	dep	AA	1148:1149	arg1	pectin					1248:1253	18 wt% pectin	1241:1253	18 wt% pectin	1241:1253	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	37	dep	AA	1148:1149	arg1	ratio					1160:1164	AM molar ratio	1151:1164	AM molar ratio	1151:1164	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	7	38	theme	equilibrium	999:1009	arg1	ESR					1027:1029	ESR	1027:1029	ESR	1027:1029	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	7	38	theme	equilibrium	999:1009	arg1	ratio					1020:1024	equilibrium swelling ratio	999:1024	equilibrium swelling ratio (ESR)	999:1030	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	8	39	theme	drug	1331:1334	arg1	92.5					1349:1352	92.5	1349:1352	92.5	1349:1352	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	39	theme	drug	1331:1334	arg1	%					1344:1344	a drug release %	1329:1344	a drug release % of 92.5	1329:1352	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	39	theme	drug	1331:1334	arg1	27.58					1319:1323	27.58	1319:1323	27.58	1319:1323	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	39	theme	drug	1331:1334	arg1	17.75					1296:1300	17.75	1296:1300	17.75	1296:1300	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	40	theme	initiator	1208:1216	arg1	%					1198:1198	1%	1197:1198	1% each of initiator and MBA concentration	1197:1238	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	40	theme	initiator	1208:1216	arg1	concentration					1226:1238	MBA concentration	1222:1238	MBA concentration	1222:1238	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	40	theme	initiator	1208:1216	arg1	AA					1148:1149	5:1 AA	1144:1149	5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG	1144:1267	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	40	theme	initiator	1208:1216	arg1	APG					1265:1267	2 wt% APG	1259:1267	2 wt% APG	1259:1267	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	40	theme	initiator	1208:1216	arg1	concentration					1182:1194	25 wt% monomer concentration	1167:1194	25 wt% monomer concentration	1167:1194	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	40	theme	initiator	1208:1216	arg1	initiator					1208:1216	initiator	1208:1216	initiator	1208:1216	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	40	theme	initiator	1208:1216	arg1	pectin					1248:1253	18 wt% pectin	1241:1253	18 wt% pectin	1241:1253	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	40	theme	initiator	1208:1216	arg1	ratio					1160:1164	AM molar ratio	1151:1164	AM molar ratio	1151:1164	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	1	41	theme	crosslink	227:235	arg1	copolymerization					237:252	free radical crosslink copolymerization	214:252	free radical crosslink copolymerization of acrylic acid (AA) and acrylamide (AM)	214:293	Pectin grafted polyacrylic copolymer hydrogels were made by free radical crosslink copolymerization of acrylic acid (AA) and acrylamide (AM) in an aqueous solution of pectin.
34536479	0	42	dep	pectin	10:15	arg1	filler					33:38	filler	33:38	filler	33:38	Effect of pectin and attapulgite filler on swelling, network parameters and controlled release of diltiazem hydrochloride from polyacrylic copolymer gel.
34536479	8	43	theme	MBA	1222:1224	arg1	concentration					1226:1238	MBA concentration	1222:1238	MBA concentration	1222:1238	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	44	theme	7.4	1382:1384	arg1	pH					1376:1377	a solution pH	1365:1377	a solution pH of 7.4	1365:1384	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	45	theme	92.5	1349:1352	arg1	92.5					1349:1352	92.5	1349:1352	92.5	1349:1352	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	45	theme	92.5	1349:1352	arg1	17.75					1296:1300	17.75	1296:1300	17.75	1296:1300	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	45	theme	92.5	1349:1352	arg1	loading					1308:1314	drug loading	1303:1314	drug loading of 27.58	1303:1323	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	45	theme	92.5	1349:1352	arg1	%					1344:1344	a drug release %	1329:1344	a drug release % of 92.5	1329:1352	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	45	theme	92.5	1349:1352	arg1	27.58					1319:1323	27.58	1319:1323	27.58	1319:1323	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	45	theme	92.5	1349:1352	arg1	ESR					1289:1291	an optimized ESR	1276:1291	an optimized ESR of 17.75	1276:1300	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	2	46	theme	bis	343:345	arg1	crosslinker					378:388	a crosslinker	376:388	a crosslinker	376:388	N'N-methylene bis acrylamide (MBA) was used as a crosslinker.
34536479	2	46	theme	bis	343:345	arg1	MBA					359:361	MBA	359:361	MBA	359:361	N'N-methylene bis acrylamide (MBA) was used as a crosslinker.
34536479	2	46	theme	bis	343:345	arg1	acrylamide					347:356	N'N-methylene bis acrylamide	329:356	N'N-methylene bis acrylamide (MBA)	329:362	N'N-methylene bis acrylamide (MBA) was used as a crosslinker.
34536479	1	47	theme	acid	265:268	arg1	copolymerization					237:252	free radical crosslink copolymerization	214:252	free radical crosslink copolymerization of acrylic acid (AA) and acrylamide (AM)	214:293	Pectin grafted polyacrylic copolymer hydrogels were made by free radical crosslink copolymerization of acrylic acid (AA) and acrylamide (AM) in an aqueous solution of pectin.
34536479	4	48	theme	filled	532:537	arg1	hydrogels					539:547	Several filled hydrogels	524:547	Several filled hydrogels	524:547	Several filled hydrogels were prepared by varying the amount of pectin and APG filler.
34536479	7	49	theme	methodology	976:986	arg1	model					950:954	a central composite design (CCD) model	917:954	a central composite design (CCD) model of response surface methodology (RSM)	917:992	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	6	50	theme	hydrochloride	814:826	arg1	kinetics					779:786	The release kinetics	767:786	The release kinetics of a model drug diltiazem hydrochloride (DT)	767:831	The release kinetics of a model drug diltiazem hydrochloride (DT) was studied with these hydrogels.
34536479	0	51	from	gel	149:151	arg1	parameters					61:70	network parameters	53:70	network parameters	53:70	Effect of pectin and attapulgite filler on swelling, network parameters and controlled release of diltiazem hydrochloride from polyacrylic copolymer gel.
34536479	0	51	from	gel	149:151	arg1	release					87:93	controlled release	76:93	controlled release of diltiazem hydrochloride from polyacrylic copolymer gel	76:151	Effect of pectin and attapulgite filler on swelling, network parameters and controlled release of diltiazem hydrochloride from polyacrylic copolymer gel.
34536479	0	51	from	gel	149:151	arg1	swelling					43:50	swelling	43:50	swelling	43:50	Effect of pectin and attapulgite filler on swelling, network parameters and controlled release of diltiazem hydrochloride from polyacrylic copolymer gel.
34536479	7	52	theme	response	959:966	arg1	RSM					989:991	RSM	989:991	RSM	989:991	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	7	52	theme	response	959:966	arg1	methodology					976:986	response surface methodology	959:986	response surface methodology (RSM)	959:992	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	1	53	theme	polyacrylic	169:179	arg1	hydrogels					191:199	Pectin grafted polyacrylic copolymer hydrogels	154:199	Pectin grafted polyacrylic copolymer hydrogels	154:199	Pectin grafted polyacrylic copolymer hydrogels were made by free radical crosslink copolymerization of acrylic acid (AA) and acrylamide (AM) in an aqueous solution of pectin.
34536479	6	54	theme	drug	799:802	arg1	DT					829:830	DT	829:830	DT	829:830	The release kinetics of a model drug diltiazem hydrochloride (DT) was studied with these hydrogels.
34536479	6	54	theme	drug	799:802	arg1	hydrochloride					814:826	a model drug diltiazem hydrochloride	791:826	a model drug diltiazem hydrochloride (DT)	791:831	The release kinetics of a model drug diltiazem hydrochloride (DT) was studied with these hydrogels.
34536479	4	55	theme	filler	603:608	arg1	pectin					588:593	pectin	588:593	pectin	588:593	Several filled hydrogels were prepared by varying the amount of pectin and APG filler.
34536479	4	55	theme	filler	603:608	arg1	filler					603:608	APG filler	599:608	APG filler	599:608	Several filled hydrogels were prepared by varying the amount of pectin and APG filler.
34536479	4	55	theme	filler	603:608	arg1	amount					578:583	the amount	574:583	the amount of pectin and APG filler	574:608	Several filled hydrogels were prepared by varying the amount of pectin and APG filler.
34536479	7	56	theme	CCD	945:947	arg1	model					950:954	a central composite design (CCD) model	917:954	a central composite design (CCD) model of response surface methodology (RSM)	917:992	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	3	57	theme	copolymer	509:517	arg1	gel					519:521	the copolymer gel	505:521	the copolymer gel	505:521	During the polymerization reaction the attapulgite (APG) filler was also incorporated in situ into the network of the copolymer gel.
34536479	5	58	theme	network	747:753	arg1	parameters					755:764	network parameters	747:764	network parameters	747:764	These hydrogels were characterized by FTIR, 13C NMR, XRD, TGA, SEM, mechanical properties, DMA, swelling, diffusion characteristics and network parameters.
34536479	0	59	theme	copolymer	139:147	arg1	gel					149:151	polyacrylic copolymer gel	127:151	polyacrylic copolymer gel	127:151	Effect of pectin and attapulgite filler on swelling, network parameters and controlled release of diltiazem hydrochloride from polyacrylic copolymer gel.
34536479	1	60	theme	free	214:217	arg1	copolymerization					237:252	free radical crosslink copolymerization	214:252	free radical crosslink copolymerization of acrylic acid (AA) and acrylamide (AM)	214:293	Pectin grafted polyacrylic copolymer hydrogels were made by free radical crosslink copolymerization of acrylic acid (AA) and acrylamide (AM) in an aqueous solution of pectin.
34536479	8	61	theme	%	1246:1246	arg1	AA					1148:1149	5:1 AA	1144:1149	5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG	1144:1267	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	61	theme	%	1246:1246	arg1	pectin					1248:1253	18 wt% pectin	1241:1253	18 wt% pectin	1241:1253	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	7	62	theme	central	919:925	arg1	model					950:954	a central composite design (CCD) model	917:954	a central composite design (CCD) model of response surface methodology (RSM)	917:992	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	7	63	theme	MBA	894:896	arg1	MBA					894:896	MBA	894:896	MBA	894:896	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	7	63	theme	MBA	894:896	arg1	APG					886:888	APG	886:888	APG	886:888	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	7	63	theme	MBA	894:896	arg1	pectin					878:883	pectin	878:883	pectin	878:883	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	7	63	theme	MBA	894:896	arg1	%					873:873	The wt%	867:873	The wt% of pectin, APG and MBA	867:896	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	8	64	theme	%	1263:1263	arg1	APG					1265:1267	2 wt% APG	1259:1267	2 wt% APG	1259:1267	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	64	theme	%	1263:1263	arg1	AA					1148:1149	5:1 AA	1144:1149	5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG	1144:1267	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	0	65	theme	network	53:59	arg1	parameters					61:70	network parameters	53:70	network parameters	53:70	Effect of pectin and attapulgite filler on swelling, network parameters and controlled release of diltiazem hydrochloride from polyacrylic copolymer gel.
34536479	7	66	theme	APG	886:888	arg1	MBA					894:896	MBA	894:896	MBA	894:896	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	7	66	theme	APG	886:888	arg1	APG					886:888	APG	886:888	APG	886:888	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	7	66	theme	APG	886:888	arg1	pectin					878:883	pectin	878:883	pectin	878:883	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	7	66	theme	APG	886:888	arg1	%					873:873	The wt%	867:873	The wt% of pectin, APG and MBA	867:896	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	7	67	theme	mg/100 mg	1050:1058	arg1	adsorption					1038:1047	drug adsorption	1033:1047	drug adsorption (mg/100 mg gel)	1033:1063	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	7	67	theme	mg/100 mg	1050:1058	arg1	gel					1060:1062	mg/100 mg gel	1050:1062	mg/100 mg gel	1050:1062	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	5	68	theme	mechanical	679:688	arg1	properties					690:699	mechanical properties	679:699	mechanical properties	679:699	These hydrogels were characterized by FTIR, 13C NMR, XRD, TGA, SEM, mechanical properties, DMA, swelling, diffusion characteristics and network parameters.
34536479	7	69	theme	pectin	878:883	arg1	MBA					894:896	MBA	894:896	MBA	894:896	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	7	69	theme	pectin	878:883	arg1	APG					886:888	APG	886:888	APG	886:888	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	7	69	theme	pectin	878:883	arg1	pectin					878:883	pectin	878:883	pectin	878:883	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	7	69	theme	pectin	878:883	arg1	%					873:873	The wt%	867:873	The wt% of pectin, APG and MBA	867:896	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	1	70	theme	Pectin	154:159	arg1	hydrogels					191:199	Pectin grafted polyacrylic copolymer hydrogels	154:199	Pectin grafted polyacrylic copolymer hydrogels	154:199	Pectin grafted polyacrylic copolymer hydrogels were made by free radical crosslink copolymerization of acrylic acid (AA) and acrylamide (AM) in an aqueous solution of pectin.
34536479	0	71	theme	hydrochloride	108:120	arg1	parameters					61:70	network parameters	53:70	network parameters	53:70	Effect of pectin and attapulgite filler on swelling, network parameters and controlled release of diltiazem hydrochloride from polyacrylic copolymer gel.
34536479	0	71	theme	hydrochloride	108:120	arg1	release					87:93	controlled release	76:93	controlled release of diltiazem hydrochloride from polyacrylic copolymer gel	76:151	Effect of pectin and attapulgite filler on swelling, network parameters and controlled release of diltiazem hydrochloride from polyacrylic copolymer gel.
34536479	0	71	theme	hydrochloride	108:120	arg1	swelling					43:50	swelling	43:50	swelling	43:50	Effect of pectin and attapulgite filler on swelling, network parameters and controlled release of diltiazem hydrochloride from polyacrylic copolymer gel.
34536479	8	72	theme	optimized	1279:1287	arg1	ESR					1289:1291	an optimized ESR	1276:1291	an optimized ESR of 17.75	1276:1300	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	73	theme	AM	1151:1152	arg1	ratio					1160:1164	AM molar ratio	1151:1164	AM molar ratio	1151:1164	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	73	theme	AM	1151:1152	arg1	AA					1148:1149	5:1 AA	1144:1149	5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG	1144:1267	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	0	74	theme	diltiazem	98:106	arg1	hydrochloride					108:120	diltiazem hydrochloride	98:120	diltiazem hydrochloride	98:120	Effect of pectin and attapulgite filler on swelling, network parameters and controlled release of diltiazem hydrochloride from polyacrylic copolymer gel.
34536479	7	75	theme	drug	1033:1036	arg1	adsorption					1038:1047	drug adsorption	1033:1047	drug adsorption (mg/100 mg gel)	1033:1063	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	7	75	theme	drug	1033:1036	arg1	gel					1060:1062	mg/100 mg gel	1050:1062	mg/100 mg gel	1050:1062	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	7	76	theme	design	937:942	arg1	model					950:954	a central composite design (CCD) model	917:954	a central composite design (CCD) model of response surface methodology (RSM)	917:992	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	8	77	theme	25 wt	1167:1171	arg1	AA					1148:1149	5:1 AA	1144:1149	5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG	1144:1267	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	77	theme	25 wt	1167:1171	arg1	concentration					1182:1194	25 wt% monomer concentration	1167:1194	25 wt% monomer concentration	1167:1194	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	1	78	theme	acrylamide	279:288	arg1	copolymerization					237:252	free radical crosslink copolymerization	214:252	free radical crosslink copolymerization of acrylic acid (AA) and acrylamide (AM)	214:293	Pectin grafted polyacrylic copolymer hydrogels were made by free radical crosslink copolymerization of acrylic acid (AA) and acrylamide (AM) in an aqueous solution of pectin.
34536479	8	79	theme	27.58	1319:1323	arg1	92.5					1349:1352	92.5	1349:1352	92.5	1349:1352	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	79	theme	27.58	1319:1323	arg1	17.75					1296:1300	17.75	1296:1300	17.75	1296:1300	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	79	theme	27.58	1319:1323	arg1	loading					1308:1314	drug loading	1303:1314	drug loading of 27.58	1303:1323	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	79	theme	27.58	1319:1323	arg1	%					1344:1344	a drug release %	1329:1344	a drug release % of 92.5	1329:1352	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	79	theme	27.58	1319:1323	arg1	27.58					1319:1323	27.58	1319:1323	27.58	1319:1323	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	79	theme	27.58	1319:1323	arg1	ESR					1289:1291	an optimized ESR	1276:1291	an optimized ESR of 17.75	1276:1300	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	80	theme	monomer	1174:1180	arg1	AA					1148:1149	5:1 AA	1144:1149	5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG	1144:1267	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	80	theme	monomer	1174:1180	arg1	concentration					1182:1194	25 wt% monomer concentration	1167:1194	25 wt% monomer concentration	1167:1194	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	3	81	theme	APG	443:445	arg1	filler					448:453	the attapulgite (APG) filler	426:453	the attapulgite (APG) filler	426:453	During the polymerization reaction the attapulgite (APG) filler was also incorporated in situ into the network of the copolymer gel.
34536479	7	82	theme	swelling	1011:1018	arg1	ESR					1027:1029	ESR	1027:1029	ESR	1027:1029	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	7	82	theme	swelling	1011:1018	arg1	ratio					1020:1024	equilibrium swelling ratio	999:1024	equilibrium swelling ratio (ESR)	999:1030	The wt% of pectin, APG and MBA was optimized with a central composite design (CCD) model of response surface methodology (RSM) with equilibrium swelling ratio (ESR), drug adsorption (mg/100 mg gel) and drug release% in 16 h as response.
34536479	3	83	theme	attapulgite	430:440	arg1	filler					448:453	the attapulgite (APG) filler	426:453	the attapulgite (APG) filler	426:453	During the polymerization reaction the attapulgite (APG) filler was also incorporated in situ into the network of the copolymer gel.
34536479	6	84	theme	release	771:777	arg1	kinetics					779:786	The release kinetics	767:786	The release kinetics of a model drug diltiazem hydrochloride (DT)	767:831	The release kinetics of a model drug diltiazem hydrochloride (DT) was studied with these hydrogels.
34536479	8	85	theme	release	1336:1342	arg1	92.5					1349:1352	92.5	1349:1352	92.5	1349:1352	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	85	theme	release	1336:1342	arg1	%					1344:1344	a drug release %	1329:1344	a drug release % of 92.5	1329:1352	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	85	theme	release	1336:1342	arg1	27.58					1319:1323	27.58	1319:1323	27.58	1319:1323	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	8	85	theme	release	1336:1342	arg1	17.75					1296:1300	17.75	1296:1300	17.75	1296:1300	Accordingly, the hydrogel prepared with 5:1 AA:AM molar ratio, 25 wt% monomer concentration, 1% each of initiator and MBA concentration, 18 wt% pectin and 2 wt% APG showed an optimized ESR of 17.75, drug loading of 27.58 and a drug release % of 92.5 in 16 h at a solution pH of 7.4.
34536479	1	86	theme	aqueous	301:307	arg1	solution					309:316	an aqueous solution	298:316	an aqueous solution of pectin	298:326	Pectin grafted polyacrylic copolymer hydrogels were made by free radical crosslink copolymerization of acrylic acid (AA) and acrylamide (AM) in an aqueous solution of pectin.
34536479	1	87	theme	acrylic	257:263	arg1	AA					271:272	AA	271:272	AA	271:272	Pectin grafted polyacrylic copolymer hydrogels were made by free radical crosslink copolymerization of acrylic acid (AA) and acrylamide (AM) in an aqueous solution of pectin.
34536479	1	87	theme	acrylic	257:263	arg1	acid					265:268	acrylic acid	257:268	acrylic acid (AA)	257:273	Pectin grafted polyacrylic copolymer hydrogels were made by free radical crosslink copolymerization of acrylic acid (AA) and acrylamide (AM) in an aqueous solution of pectin.
33289129	2	0	dep	ex	466:467	arg1	situ					469:472	situ	469:472	situ	469:472	Highly dispersed synthesized C-dots with a size smaller than 10 nm (approximately 4.9 nm) were impregnated into BNC by an ex situ coating method and then mechanical, morphological, UV-protectant and antibacterial activity were assessed.
33289129	1	1	theme	BACKGROUND	146:155	arg1	C-dots					170:175	C-dots	170:175	C-dots	170:175	BACKGROUND Carbon dots (C-dots) with antimicrobial activity were synthesized from the white mulberry extract with the aim of fabricating anti-listeria nanopaper using bacterial nanocellulose (BNC).
33289129	1	1	theme	BACKGROUND	146:155	arg1	dots					164:167	BACKGROUND Carbon dots	146:167	BACKGROUND Carbon dots (C-dots) with antimicrobial activity	146:204	BACKGROUND Carbon dots (C-dots) with antimicrobial activity were synthesized from the white mulberry extract with the aim of fabricating anti-listeria nanopaper using bacterial nanocellulose (BNC).
33289129	1	2	theme	mulberry	238:245	arg1	extract					247:253	the white mulberry extract	228:253	the white mulberry extract	228:253	BACKGROUND Carbon dots (C-dots) with antimicrobial activity were synthesized from the white mulberry extract with the aim of fabricating anti-listeria nanopaper using bacterial nanocellulose (BNC).
33289129	0	3	theme	carbon	99:104	arg1	dots					106:109	carbon dots	99:109	carbon dots of white mulberry (Morus alba L.)	99:143	Optimization and characterization of eco-friendly antimicrobial nanocellulose sheet prepared using carbon dots of white mulberry (Morus alba L.).
33289129	1	4	theme	Carbon	157:162	arg1	C-dots					170:175	C-dots	170:175	C-dots	170:175	BACKGROUND Carbon dots (C-dots) with antimicrobial activity were synthesized from the white mulberry extract with the aim of fabricating anti-listeria nanopaper using bacterial nanocellulose (BNC).
33289129	1	4	theme	Carbon	157:162	arg1	dots					164:167	BACKGROUND Carbon dots	146:167	BACKGROUND Carbon dots (C-dots) with antimicrobial activity	146:204	BACKGROUND Carbon dots (C-dots) with antimicrobial activity were synthesized from the white mulberry extract with the aim of fabricating anti-listeria nanopaper using bacterial nanocellulose (BNC).
33289129	6	5	with	sheet	1081:1085	arg1	property					1119:1126	high-efficient UV-blocking property	1092:1126	high-efficient UV-blocking property	1092:1126	A BNC sheet with high-efficient UV-blocking property was prepared using C-dots.
33289129	0	6	theme	white	114:118	arg1	L					141:141	Morus alba L.	130:142	Morus alba L.	130:142	Optimization and characterization of eco-friendly antimicrobial nanocellulose sheet prepared using carbon dots of white mulberry (Morus alba L.).
33289129	0	6	theme	white	114:118	arg1	mulberry					120:127	white mulberry	114:127	white mulberry (Morus alba L.)	114:143	Optimization and characterization of eco-friendly antimicrobial nanocellulose sheet prepared using carbon dots of white mulberry (Morus alba L.).
33289129	3	7	theme	optimized	685:693	arg1	concentration					695:707	the optimized concentration	681:707	the optimized concentration of C-dots in the BNC membrane	681:737	Randomized response surface methodology using a central composite design was applied to investigate the optimized concentration of C-dots in the BNC membrane.
33289129	4	8	theme	antimicrobial	879:891	arg1	activity					893:900	significant antimicrobial activity	867:900	significant antimicrobial activity on Listeria monocytogenes	867:926	RESULTS An optimized nanopaper including C-dots at a concentration of 530 g L-1 and an impregnation time of 14 h at 30 °C with significant antimicrobial activity on Listeria monocytogenes was designed.
33289129	2	9	dep	dispersed	351:359	arg1	synthesized					361:371	synthesized	361:371	dispersed synthesized C-dots with a size smaller than 10 nm (approximately 4.9 nm) were impregnated into BNC by an ex situ coating method and then mechanical, morphological, UV-protectant and antibacterial activity were assessed	351:578	Highly dispersed synthesized C-dots with a size smaller than 10 nm (approximately 4.9 nm) were impregnated into BNC by an ex situ coating method and then mechanical, morphological, UV-protectant and antibacterial activity were assessed.
33289129	4	10	theme	significant	867:877	arg1	activity					893:900	significant antimicrobial activity	867:900	significant antimicrobial activity on Listeria monocytogenes	867:926	RESULTS An optimized nanopaper including C-dots at a concentration of 530 g L-1 and an impregnation time of 14 h at 30 °C with significant antimicrobial activity on Listeria monocytogenes was designed.
33289129	4	11	theme	optimized	751:759	arg1	nanopaper					761:769	An optimized nanopaper	748:769	An optimized nanopaper including C-dots at a concentration of 530 g L-1 and an impregnation time of 14 h at 30 °C with significant antimicrobial activity on Listeria monocytogenes	748:926	RESULTS An optimized nanopaper including C-dots at a concentration of 530 g L-1 and an impregnation time of 14 h at 30 °C with significant antimicrobial activity on Listeria monocytogenes was designed.
33289129	3	12	theme	BNC	726:728	arg1	membrane					730:737	the BNC membrane	722:737	the BNC membrane	722:737	Randomized response surface methodology using a central composite design was applied to investigate the optimized concentration of C-dots in the BNC membrane.
33289129	2	13	theme	morphological	510:522	arg1	activity					557:564	then mechanical, morphological, UV-protectant and antibacterial activity	493:564	then mechanical, morphological, UV-protectant and antibacterial activity	493:564	Highly dispersed synthesized C-dots with a size smaller than 10 nm (approximately 4.9 nm) were impregnated into BNC by an ex situ coating method and then mechanical, morphological, UV-protectant and antibacterial activity were assessed.
33289129	6	14	theme	high-efficient	1092:1105	arg1	property					1119:1126	high-efficient UV-blocking property	1092:1126	high-efficient UV-blocking property	1092:1126	A BNC sheet with high-efficient UV-blocking property was prepared using C-dots.
33289129	2	15	theme	smaller	392:398	arg1	size					387:390	a size	385:390	a size smaller than 10 nm (approximately 4.9 nm)	385:432	Highly dispersed synthesized C-dots with a size smaller than 10 nm (approximately 4.9 nm) were impregnated into BNC by an ex situ coating method and then mechanical, morphological, UV-protectant and antibacterial activity were assessed.
33289129	2	15	theme	smaller	392:398	arg1	nm					430:431	approximately 4.9 nm	412:431	approximately 4.9 nm	412:431	Highly dispersed synthesized C-dots with a size smaller than 10 nm (approximately 4.9 nm) were impregnated into BNC by an ex situ coating method and then mechanical, morphological, UV-protectant and antibacterial activity were assessed.
33289129	0	16	theme	mulberry	120:127	arg1	dots					106:109	carbon dots	99:109	carbon dots of white mulberry (Morus alba L.)	99:143	Optimization and characterization of eco-friendly antimicrobial nanocellulose sheet prepared using carbon dots of white mulberry (Morus alba L.).
33289129	3	17	theme	C-dots	712:717	arg1	concentration					695:707	the optimized concentration	681:707	the optimized concentration of C-dots in the BNC membrane	681:737	Randomized response surface methodology using a central composite design was applied to investigate the optimized concentration of C-dots in the BNC membrane.
33289129	5	18	theme	ultimate	998:1005	arg1	strength					1015:1022	ultimate tensile strength	998:1022	ultimate tensile strength	998:1022	The addition of C-dots into BNC significantly increased ultimate tensile strength and decreased strain with respect to breaking BNC.
33289129	7	19	theme	designed	1192:1199	arg1	CONCLUSION					1155:1164	CONCLUSION	1155:1164	CONCLUSION Based on the results	1155:1185	CONCLUSION Based on the results, the designed nanopaper shows a substantial capacity with respect to the fabrication of antimicrobial/UV-blocking sheets for food active packaging.
33289129	7	19	theme	designed	1192:1199	arg1	nanopaper					1201:1209	the designed nanopaper	1188:1209	the designed nanopaper	1188:1209	CONCLUSION Based on the results, the designed nanopaper shows a substantial capacity with respect to the fabrication of antimicrobial/UV-blocking sheets for food active packaging.
33289129	4	20	theme	Listeria	905:912	arg1	monocytogenes					914:926	Listeria monocytogenes	905:926	Listeria monocytogenes	905:926	RESULTS An optimized nanopaper including C-dots at a concentration of 530 g L-1 and an impregnation time of 14 h at 30 °C with significant antimicrobial activity on Listeria monocytogenes was designed.
33289129	4	21	theme	14 h	848:851	arg1	concentration					793:805	a concentration	791:805	a concentration of 530 g L-1	791:818	RESULTS An optimized nanopaper including C-dots at a concentration of 530 g L-1 and an impregnation time of 14 h at 30 °C with significant antimicrobial activity on Listeria monocytogenes was designed.
33289129	4	21	theme	14 h	848:851	arg1	time					840:843	an impregnation time	824:843	an impregnation time of 14 h	824:851	RESULTS An optimized nanopaper including C-dots at a concentration of 530 g L-1 and an impregnation time of 14 h at 30 °C with significant antimicrobial activity on Listeria monocytogenes was designed.
33289129	6	22	theme	BNC	1077:1079	arg1	sheet					1081:1085	A BNC sheet	1075:1085	A BNC sheet with high-efficient UV-blocking property	1075:1126	A BNC sheet with high-efficient UV-blocking property was prepared using C-dots.
33289129	0	23	theme	alba	136:139	arg1	L					141:141	Morus alba L.	130:142	Morus alba L.	130:142	Optimization and characterization of eco-friendly antimicrobial nanocellulose sheet prepared using carbon dots of white mulberry (Morus alba L.).
33289129	0	23	theme	alba	136:139	arg1	mulberry					120:127	white mulberry	114:127	white mulberry (Morus alba L.)	114:143	Optimization and characterization of eco-friendly antimicrobial nanocellulose sheet prepared using carbon dots of white mulberry (Morus alba L.).
33289129	5	24	theme	C-dots	958:963	arg1	addition					946:953	The addition	942:953	The addition of C-dots into BNC	942:972	The addition of C-dots into BNC significantly increased ultimate tensile strength and decreased strain with respect to breaking BNC.
33289129	1	25	theme	fabricating	271:281	arg1	nanopaper					297:305	fabricating anti-listeria nanopaper	271:305	fabricating anti-listeria nanopaper using bacterial nanocellulose (BNC)	271:341	BACKGROUND Carbon dots (C-dots) with antimicrobial activity were synthesized from the white mulberry extract with the aim of fabricating anti-listeria nanopaper using bacterial nanocellulose (BNC).
33289129	0	26	theme	Morus	130:134	arg1	L					141:141	Morus alba L.	130:142	Morus alba L.	130:142	Optimization and characterization of eco-friendly antimicrobial nanocellulose sheet prepared using carbon dots of white mulberry (Morus alba L.).
33289129	0	26	theme	Morus	130:134	arg1	mulberry					120:127	white mulberry	114:127	white mulberry (Morus alba L.)	114:143	Optimization and characterization of eco-friendly antimicrobial nanocellulose sheet prepared using carbon dots of white mulberry (Morus alba L.).
33289129	3	27	theme	composite	637:645	arg1	design					647:652	a central composite design	627:652	a central composite design	627:652	Randomized response surface methodology using a central composite design was applied to investigate the optimized concentration of C-dots in the BNC membrane.
33289129	6	28	theme	UV-blocking	1107:1117	arg1	property					1119:1126	high-efficient UV-blocking property	1092:1126	high-efficient UV-blocking property	1092:1126	A BNC sheet with high-efficient UV-blocking property was prepared using C-dots.
33289129	1	29	theme	antimicrobial	183:195	arg1	activity					197:204	antimicrobial activity	183:204	antimicrobial activity	183:204	BACKGROUND Carbon dots (C-dots) with antimicrobial activity were synthesized from the white mulberry extract with the aim of fabricating anti-listeria nanopaper using bacterial nanocellulose (BNC).
33289129	0	30	theme	antimicrobial	50:62	arg1	sheet					78:82	eco-friendly antimicrobial nanocellulose sheet	37:82	eco-friendly antimicrobial nanocellulose sheet	37:82	Optimization and characterization of eco-friendly antimicrobial nanocellulose sheet prepared using carbon dots of white mulberry (Morus alba L.).
33289129	1	31	theme	anti-listeria	283:295	arg1	nanopaper					297:305	fabricating anti-listeria nanopaper	271:305	fabricating anti-listeria nanopaper using bacterial nanocellulose (BNC)	271:341	BACKGROUND Carbon dots (C-dots) with antimicrobial activity were synthesized from the white mulberry extract with the aim of fabricating anti-listeria nanopaper using bacterial nanocellulose (BNC).
33289129	0	32	theme	eco-friendly	37:48	arg1	sheet					78:82	eco-friendly antimicrobial nanocellulose sheet	37:82	eco-friendly antimicrobial nanocellulose sheet	37:82	Optimization and characterization of eco-friendly antimicrobial nanocellulose sheet prepared using carbon dots of white mulberry (Morus alba L.).
33289129	1	33	theme	nanopaper	297:305	arg1	aim					264:266	the aim	260:266	the aim of fabricating anti-listeria nanopaper using bacterial nanocellulose (BNC)	260:341	BACKGROUND Carbon dots (C-dots) with antimicrobial activity were synthesized from the white mulberry extract with the aim of fabricating anti-listeria nanopaper using bacterial nanocellulose (BNC).
33289129	2	34	theme	mechanical	498:507	arg1	activity					557:564	then mechanical, morphological, UV-protectant and antibacterial activity	493:564	then mechanical, morphological, UV-protectant and antibacterial activity	493:564	Highly dispersed synthesized C-dots with a size smaller than 10 nm (approximately 4.9 nm) were impregnated into BNC by an ex situ coating method and then mechanical, morphological, UV-protectant and antibacterial activity were assessed.
33289129	3	35	theme	Randomized	581:590	arg1	methodology					609:619	Randomized response surface methodology	581:619	Randomized response surface methodology using a central composite design	581:652	Randomized response surface methodology using a central composite design was applied to investigate the optimized concentration of C-dots in the BNC membrane.
33289129	2	36	theme	coating	474:480	arg1	method					482:487	an ex situ coating method	463:487	an ex situ coating method	463:487	Highly dispersed synthesized C-dots with a size smaller than 10 nm (approximately 4.9 nm) were impregnated into BNC by an ex situ coating method and then mechanical, morphological, UV-protectant and antibacterial activity were assessed.
33289129	0	37	theme	sheet	78:82	arg1	characterization					17:32	characterization	17:32	characterization	17:32	Optimization and characterization of eco-friendly antimicrobial nanocellulose sheet prepared using carbon dots of white mulberry (Morus alba L.).
33289129	0	37	theme	sheet	78:82	arg1	Optimization					0:11	Optimization	0:11	Optimization	0:11	Optimization and characterization of eco-friendly antimicrobial nanocellulose sheet prepared using carbon dots of white mulberry (Morus alba L.).
33289129	7	38	theme	substantial	1219:1229	arg1	capacity					1231:1238	a substantial capacity	1217:1238	a substantial capacity	1217:1238	CONCLUSION Based on the results, the designed nanopaper shows a substantial capacity with respect to the fabrication of antimicrobial/UV-blocking sheets for food active packaging.
33289129	3	39	theme	central	629:635	arg1	design					647:652	a central composite design	627:652	a central composite design	627:652	Randomized response surface methodology using a central composite design was applied to investigate the optimized concentration of C-dots in the BNC membrane.
33289129	4	40	theme	impregnation	827:838	arg1	time					840:843	an impregnation time	824:843	an impregnation time of 14 h	824:851	RESULTS An optimized nanopaper including C-dots at a concentration of 530 g L-1 and an impregnation time of 14 h at 30 °C with significant antimicrobial activity on Listeria monocytogenes was designed.
33289129	7	41	theme	active	1317:1322	arg1	packaging					1324:1332	food active packaging	1312:1332	food active packaging	1312:1332	CONCLUSION Based on the results, the designed nanopaper shows a substantial capacity with respect to the fabrication of antimicrobial/UV-blocking sheets for food active packaging.
33289129	0	42	theme	nanocellulose	64:76	arg1	sheet					78:82	eco-friendly antimicrobial nanocellulose sheet	37:82	eco-friendly antimicrobial nanocellulose sheet	37:82	Optimization and characterization of eco-friendly antimicrobial nanocellulose sheet prepared using carbon dots of white mulberry (Morus alba L.).
33289129	1	43	theme	bacterial	313:321	arg1	BNC					338:340	BNC	338:340	BNC	338:340	BACKGROUND Carbon dots (C-dots) with antimicrobial activity were synthesized from the white mulberry extract with the aim of fabricating anti-listeria nanopaper using bacterial nanocellulose (BNC).
33289129	1	43	theme	bacterial	313:321	arg1	nanocellulose					323:335	bacterial nanocellulose	313:335	bacterial nanocellulose (BNC)	313:341	BACKGROUND Carbon dots (C-dots) with antimicrobial activity were synthesized from the white mulberry extract with the aim of fabricating anti-listeria nanopaper using bacterial nanocellulose (BNC).
33289129	2	44	theme	antibacterial	543:555	arg1	activity					557:564	then mechanical, morphological, UV-protectant and antibacterial activity	493:564	then mechanical, morphological, UV-protectant and antibacterial activity	493:564	Highly dispersed synthesized C-dots with a size smaller than 10 nm (approximately 4.9 nm) were impregnated into BNC by an ex situ coating method and then mechanical, morphological, UV-protectant and antibacterial activity were assessed.
33289129	5	45	theme	breaking	1061:1068	arg1	BNC					1070:1072	breaking BNC	1061:1072	breaking BNC	1061:1072	The addition of C-dots into BNC significantly increased ultimate tensile strength and decreased strain with respect to breaking BNC.
33289129	4	46	dep	RESULTS	740:746	arg1	designed					932:939	designed	932:939	was designed	928:939	RESULTS An optimized nanopaper including C-dots at a concentration of 530 g L-1 and an impregnation time of 14 h at 30 °C with significant antimicrobial activity on Listeria monocytogenes was designed.
33289129	1	47	with	dots	164:167	arg1	activity					197:204	antimicrobial activity	183:204	antimicrobial activity	183:204	BACKGROUND Carbon dots (C-dots) with antimicrobial activity were synthesized from the white mulberry extract with the aim of fabricating anti-listeria nanopaper using bacterial nanocellulose (BNC).
33289129	5	48	theme	tensile	1007:1013	arg1	strength					1015:1022	ultimate tensile strength	998:1022	ultimate tensile strength	998:1022	The addition of C-dots into BNC significantly increased ultimate tensile strength and decreased strain with respect to breaking BNC.
33289129	4	49	theme	530 g L-1	810:818	arg1	concentration					793:805	a concentration	791:805	a concentration of 530 g L-1	791:818	RESULTS An optimized nanopaper including C-dots at a concentration of 530 g L-1 and an impregnation time of 14 h at 30 °C with significant antimicrobial activity on Listeria monocytogenes was designed.
33289129	4	49	theme	530 g L-1	810:818	arg1	time					840:843	an impregnation time	824:843	an impregnation time of 14 h	824:851	RESULTS An optimized nanopaper including C-dots at a concentration of 530 g L-1 and an impregnation time of 14 h at 30 °C with significant antimicrobial activity on Listeria monocytogenes was designed.
33289129	3	50	from	concentration	695:707	arg1	membrane					730:737	the BNC membrane	722:737	the BNC membrane	722:737	Randomized response surface methodology using a central composite design was applied to investigate the optimized concentration of C-dots in the BNC membrane.
33289129	2	51	theme	UV-protectant	525:537	arg1	activity					557:564	then mechanical, morphological, UV-protectant and antibacterial activity	493:564	then mechanical, morphological, UV-protectant and antibacterial activity	493:564	Highly dispersed synthesized C-dots with a size smaller than 10 nm (approximately 4.9 nm) were impregnated into BNC by an ex situ coating method and then mechanical, morphological, UV-protectant and antibacterial activity were assessed.
33289129	8	52	theme	Chemical	1353:1360	arg1	Industry					1362:1369	Chemical Industry	1353:1369	Chemical Industry	1353:1369	© 2020 Society of Chemical Industry.
33289129	7	53	theme	food	1312:1315	arg1	packaging					1324:1332	food active packaging	1312:1332	food active packaging	1312:1332	CONCLUSION Based on the results, the designed nanopaper shows a substantial capacity with respect to the fabrication of antimicrobial/UV-blocking sheets for food active packaging.
33289129	4	54	from	activity	893:900	arg1	monocytogenes					914:926	Listeria monocytogenes	905:926	Listeria monocytogenes	905:926	RESULTS An optimized nanopaper including C-dots at a concentration of 530 g L-1 and an impregnation time of 14 h at 30 °C with significant antimicrobial activity on Listeria monocytogenes was designed.
33289129	3	55	theme	response	592:599	arg1	methodology					609:619	Randomized response surface methodology	581:619	Randomized response surface methodology using a central composite design	581:652	Randomized response surface methodology using a central composite design was applied to investigate the optimized concentration of C-dots in the BNC membrane.
33289129	7	56	theme	antimicrobial/UV-blocking	1275:1299	arg1	sheets					1301:1306	antimicrobial/UV-blocking sheets	1275:1306	antimicrobial/UV-blocking sheets for food active packaging	1275:1332	CONCLUSION Based on the results, the designed nanopaper shows a substantial capacity with respect to the fabrication of antimicrobial/UV-blocking sheets for food active packaging.
33289129	4	57	with	30 °C	856:860	arg1	activity					893:900	significant antimicrobial activity	867:900	significant antimicrobial activity on Listeria monocytogenes	867:926	RESULTS An optimized nanopaper including C-dots at a concentration of 530 g L-1 and an impregnation time of 14 h at 30 °C with significant antimicrobial activity on Listeria monocytogenes was designed.
33289129	2	58	theme	ex	466:467	arg1	method					482:487	an ex situ coating method	463:487	an ex situ coating method	463:487	Highly dispersed synthesized C-dots with a size smaller than 10 nm (approximately 4.9 nm) were impregnated into BNC by an ex situ coating method and then mechanical, morphological, UV-protectant and antibacterial activity were assessed.
33289129	3	59	theme	surface	601:607	arg1	methodology					609:619	Randomized response surface methodology	581:619	Randomized response surface methodology using a central composite design	581:652	Randomized response surface methodology using a central composite design was applied to investigate the optimized concentration of C-dots in the BNC membrane.
33289129	7	60	theme	sheets	1301:1306	arg1	fabrication					1260:1270	the fabrication	1256:1270	the fabrication of antimicrobial/UV-blocking sheets for food active packaging	1256:1332	CONCLUSION Based on the results, the designed nanopaper shows a substantial capacity with respect to the fabrication of antimicrobial/UV-blocking sheets for food active packaging.
33289129	1	61	theme	white	232:236	arg1	extract					247:253	the white mulberry extract	228:253	the white mulberry extract	228:253	BACKGROUND Carbon dots (C-dots) with antimicrobial activity were synthesized from the white mulberry extract with the aim of fabricating anti-listeria nanopaper using bacterial nanocellulose (BNC).
32268322	5	0	theme	performed	817:825	arg1	tests					827:831	the performed tests	813:831	the performed tests	813:831	Moreover, the performed tests showed improved wettability and mechanical performance with the addition of bioactive glass particles.
32268322	6	1	theme	biology	992:998	arg1	tests					1000:1004	simulated body fluid and cell biology tests	962:1004	simulated body fluid and cell biology tests using relevant skin cells	962:1030	Dissolution studies using simulated body fluid and cell biology tests using relevant skin cells further proved the excellent bioactivity and positive effects of the foams on cell proliferation and migration.
32268322	7	2	theme	honey	1194:1198	arg1	release					1176:1182	the dual release	1167:1182	the dual release of Manuka honey and ions from the copper-doped bioactive glass	1167:1245	Most interestingly, by the dual release of Manuka honey and ions from the copper-doped bioactive glass, an antibacterial effect against E. coli and S. aureus was achieved.
32268322	8	3	theme	promising	1360:1368	arg1	outcomes					1370:1377	promising outcomes	1360:1377	promising outcomes	1360:1377	Therefore, the multifunctional foams showed promising outcomes as potential wound dressings for the treatment of infected wounds.
32268322	8	3	theme	promising	1360:1368	arg1	dressings					1398:1406	potential wound dressings	1382:1406	potential wound dressings for the treatment of infected wounds	1382:1443	Therefore, the multifunctional foams showed promising outcomes as potential wound dressings for the treatment of infected wounds.
32268322	4	4	theme	dual	636:639	arg1	effect					641:646	the dual effect	632:646	the dual effect of the bioactive glass and Manuka honey	632:686	The multifunctional composite providing the dual effect of the bioactive glass and Manuka honey was produced by freeze-drying, and the resulting foams exhibit suitable morphology characterized by high porosity.
32268322	6	5	theme	cell	987:990	arg1	biology					992:998	cell biology	987:998	cell biology	987:998	Dissolution studies using simulated body fluid and cell biology tests using relevant skin cells further proved the excellent bioactivity and positive effects of the foams on cell proliferation and migration.
32268322	2	6	theme	glass	374:378	arg1	particles					380:388	borate bioactive glass particles	357:388	borate bioactive glass particles doped additionally with copper	357:419	In this article, foams based on methylcellulose cross-linked with Manuka honey were used as a platform to deliver borate bioactive glass particles doped additionally with copper.
32268322	6	7	theme	positive	1077:1084	arg1	effects					1086:1092	positive effects	1077:1092	positive effects of the foams on cell proliferation and migration	1077:1141	Dissolution studies using simulated body fluid and cell biology tests using relevant skin cells further proved the excellent bioactivity and positive effects of the foams on cell proliferation and migration.
32268322	3	8	theme	Borate	422:427	arg1	glasses					439:445	Borate bioactive glasses	422:445	Borate bioactive glasses	422:445	Borate bioactive glasses are of great interest in wound-healing applications due to a combination of favorable features, such as angiogenic and antibacterial properties.
32268322	0	9	theme	wound-healing	93:105	arg1	applications					107:118	wound-healing applications	93:118	wound-healing applications	93:118	Manuka honey and bioactive glass impart methylcellulose foams with antibacterial effects for wound-healing applications.
32268322	6	10	theme	fluid	977:981	arg1	tests					1000:1004	simulated body fluid and cell biology tests	962:1004	simulated body fluid and cell biology tests using relevant skin cells	962:1030	Dissolution studies using simulated body fluid and cell biology tests using relevant skin cells further proved the excellent bioactivity and positive effects of the foams on cell proliferation and migration.
32268322	7	11	theme	Manuka	1187:1192	arg1	honey					1194:1198	Manuka honey	1187:1198	Manuka honey	1187:1198	Most interestingly, by the dual release of Manuka honey and ions from the copper-doped bioactive glass, an antibacterial effect against E. coli and S. aureus was achieved.
32268322	7	12	theme	antibacterial	1251:1263	arg1	effect					1265:1270	an antibacterial effect	1248:1270	an antibacterial effect against E. coli and S. aureus	1248:1300	Most interestingly, by the dual release of Manuka honey and ions from the copper-doped bioactive glass, an antibacterial effect against E. coli and S. aureus was achieved.
32268322	2	13	theme	bioactive	364:372	arg1	particles					380:388	borate bioactive glass particles	357:388	borate bioactive glass particles doped additionally with copper	357:419	In this article, foams based on methylcellulose cross-linked with Manuka honey were used as a platform to deliver borate bioactive glass particles doped additionally with copper.
32268322	7	14	theme	dual	1171:1174	arg1	release					1176:1182	the dual release	1167:1182	the dual release of Manuka honey and ions from the copper-doped bioactive glass	1167:1245	Most interestingly, by the dual release of Manuka honey and ions from the copper-doped bioactive glass, an antibacterial effect against E. coli and S. aureus was achieved.
32268322	4	15	theme	Manuka	675:680	arg1	honey					682:686	the bioactive glass and Manuka honey	651:686	honey	682:686	The multifunctional composite providing the dual effect of the bioactive glass and Manuka honey was produced by freeze-drying, and the resulting foams exhibit suitable morphology characterized by high porosity.
32268322	2	16	theme	Manuka	309:314	arg1	honey					316:320	Manuka honey	309:320	Manuka honey	309:320	In this article, foams based on methylcellulose cross-linked with Manuka honey were used as a platform to deliver borate bioactive glass particles doped additionally with copper.
32268322	5	17	theme	particles	925:933	arg1	addition					897:904	the addition	893:904	the addition of bioactive glass particles	893:933	Moreover, the performed tests showed improved wettability and mechanical performance with the addition of bioactive glass particles.
32268322	6	18	theme	simulated	962:970	arg1	fluid					977:981	simulated body fluid	962:981	simulated body fluid	962:981	Dissolution studies using simulated body fluid and cell biology tests using relevant skin cells further proved the excellent bioactivity and positive effects of the foams on cell proliferation and migration.
32268322	6	19	from	bioactivity	1061:1071	arg1	migration					1133:1141	migration	1133:1141	migration	1133:1141	Dissolution studies using simulated body fluid and cell biology tests using relevant skin cells further proved the excellent bioactivity and positive effects of the foams on cell proliferation and migration.
32268322	6	19	from	bioactivity	1061:1071	arg1	proliferation					1115:1127	cell proliferation	1110:1127	cell proliferation	1110:1127	Dissolution studies using simulated body fluid and cell biology tests using relevant skin cells further proved the excellent bioactivity and positive effects of the foams on cell proliferation and migration.
32268322	3	20	theme	antibacterial	566:578	arg1	properties					580:589	antibacterial properties	566:589	antibacterial properties	566:589	Borate bioactive glasses are of great interest in wound-healing applications due to a combination of favorable features, such as angiogenic and antibacterial properties.
32268322	7	21	from	glass	1241:1245	arg1	release					1176:1182	the dual release	1167:1182	the dual release of Manuka honey and ions from the copper-doped bioactive glass	1167:1245	Most interestingly, by the dual release of Manuka honey and ions from the copper-doped bioactive glass, an antibacterial effect against E. coli and S. aureus was achieved.
32268322	1	22	theme	new	195:197	arg1	generation					199:208	a new generation	193:208	a new generation of wound-regeneration therapies	193:240	Wound dressings able to deliver topically bioactive molecules represent a new generation of wound-regeneration therapies.
32268322	5	23	theme	mechanical	865:874	arg1	performance					876:886	mechanical performance	865:886	mechanical performance	865:886	Moreover, the performed tests showed improved wettability and mechanical performance with the addition of bioactive glass particles.
32268322	6	24	from	effects	1086:1092	arg1	migration					1133:1141	migration	1133:1141	migration	1133:1141	Dissolution studies using simulated body fluid and cell biology tests using relevant skin cells further proved the excellent bioactivity and positive effects of the foams on cell proliferation and migration.
32268322	6	24	from	effects	1086:1092	arg1	proliferation					1115:1127	cell proliferation	1110:1127	cell proliferation	1110:1127	Dissolution studies using simulated body fluid and cell biology tests using relevant skin cells further proved the excellent bioactivity and positive effects of the foams on cell proliferation and migration.
32268322	6	25	theme	excellent	1051:1059	arg1	bioactivity					1061:1071	the excellent bioactivity	1047:1071	the excellent bioactivity	1047:1071	Dissolution studies using simulated body fluid and cell biology tests using relevant skin cells further proved the excellent bioactivity and positive effects of the foams on cell proliferation and migration.
32268322	6	26	theme	body	972:975	arg1	fluid					977:981	simulated body fluid	962:981	simulated body fluid	962:981	Dissolution studies using simulated body fluid and cell biology tests using relevant skin cells further proved the excellent bioactivity and positive effects of the foams on cell proliferation and migration.
32268322	3	27	theme	great	454:458	arg1	interest					460:467	great interest	454:467	great interest	454:467	Borate bioactive glasses are of great interest in wound-healing applications due to a combination of favorable features, such as angiogenic and antibacterial properties.
32268322	0	28	theme	Manuka	0:5	arg1	honey					7:11	Manuka honey and bioactive glass	0:31	honey	7:11	Manuka honey and bioactive glass impart methylcellulose foams with antibacterial effects for wound-healing applications.
32268322	4	29	theme	multifunctional	596:610	arg1	composite					612:620	The multifunctional composite	592:620	The multifunctional composite providing the dual effect of the bioactive glass and Manuka honey	592:686	The multifunctional composite providing the dual effect of the bioactive glass and Manuka honey was produced by freeze-drying, and the resulting foams exhibit suitable morphology characterized by high porosity.
32268322	4	30	theme	high	788:791	arg1	porosity					793:800	high porosity	788:800	high porosity	788:800	The multifunctional composite providing the dual effect of the bioactive glass and Manuka honey was produced by freeze-drying, and the resulting foams exhibit suitable morphology characterized by high porosity.
32268322	0	31	theme	bioactive	17:25	arg1	glass					27:31	Manuka honey and bioactive glass	0:31	glass	27:31	Manuka honey and bioactive glass impart methylcellulose foams with antibacterial effects for wound-healing applications.
32268322	1	32	theme	wound-regeneration	213:230	arg1	therapies					232:240	wound-regeneration therapies	213:240	wound-regeneration therapies	213:240	Wound dressings able to deliver topically bioactive molecules represent a new generation of wound-regeneration therapies.
32268322	8	33	theme	wounds	1438:1443	arg1	treatment					1416:1424	the treatment	1412:1424	the treatment of infected wounds	1412:1443	Therefore, the multifunctional foams showed promising outcomes as potential wound dressings for the treatment of infected wounds.
32268322	8	34	theme	infected	1429:1436	arg1	wounds					1438:1443	infected wounds	1429:1443	infected wounds	1429:1443	Therefore, the multifunctional foams showed promising outcomes as potential wound dressings for the treatment of infected wounds.
32268322	1	35	theme	Wound	121:125	arg1	dressings					127:135	Wound dressings	121:135	Wound dressings able to deliver topically bioactive molecules	121:181	Wound dressings able to deliver topically bioactive molecules represent a new generation of wound-regeneration therapies.
32268322	1	36	theme	therapies	232:240	arg1	generation					199:208	a new generation	193:208	a new generation of wound-regeneration therapies	193:240	Wound dressings able to deliver topically bioactive molecules represent a new generation of wound-regeneration therapies.
32268322	2	37	theme	borate	357:362	arg1	particles					380:388	borate bioactive glass particles	357:388	borate bioactive glass particles doped additionally with copper	357:419	In this article, foams based on methylcellulose cross-linked with Manuka honey were used as a platform to deliver borate bioactive glass particles doped additionally with copper.
32268322	6	38	theme	Dissolution	936:946	arg1	studies					948:954	Dissolution studies	936:954	Dissolution studies using simulated body fluid and cell biology tests using relevant skin cells	936:1030	Dissolution studies using simulated body fluid and cell biology tests using relevant skin cells further proved the excellent bioactivity and positive effects of the foams on cell proliferation and migration.
32268322	5	39	theme	bioactive	909:917	arg1	particles					925:933	bioactive glass particles	909:933	bioactive glass particles	909:933	Moreover, the performed tests showed improved wettability and mechanical performance with the addition of bioactive glass particles.
32268322	5	40	theme	improved	840:847	arg1	wettability					849:859	improved wettability	840:859	improved wettability	840:859	Moreover, the performed tests showed improved wettability and mechanical performance with the addition of bioactive glass particles.
32268322	7	41	theme	copper-doped	1218:1229	arg1	glass					1241:1245	the copper-doped bioactive glass	1214:1245	the copper-doped bioactive glass	1214:1245	Most interestingly, by the dual release of Manuka honey and ions from the copper-doped bioactive glass, an antibacterial effect against E. coli and S. aureus was achieved.
32268322	4	42	theme	suitable	751:758	arg1	morphology					760:769	suitable morphology	751:769	suitable morphology characterized by high porosity	751:800	The multifunctional composite providing the dual effect of the bioactive glass and Manuka honey was produced by freeze-drying, and the resulting foams exhibit suitable morphology characterized by high porosity.
32268322	7	43	theme	bioactive	1231:1239	arg1	glass					1241:1245	the copper-doped bioactive glass	1214:1245	the copper-doped bioactive glass	1214:1245	Most interestingly, by the dual release of Manuka honey and ions from the copper-doped bioactive glass, an antibacterial effect against E. coli and S. aureus was achieved.
32268322	3	44	theme	due	499:501	arg1	applications					486:497	wound-healing applications	472:497	wound-healing applications due to a combination of favorable features, such as angiogenic and antibacterial properties	472:589	Borate bioactive glasses are of great interest in wound-healing applications due to a combination of favorable features, such as angiogenic and antibacterial properties.
32268322	3	45	theme	bioactive	429:437	arg1	glasses					439:445	Borate bioactive glasses	422:445	Borate bioactive glasses	422:445	Borate bioactive glasses are of great interest in wound-healing applications due to a combination of favorable features, such as angiogenic and antibacterial properties.
32268322	6	46	theme	skin	1021:1024	arg1	cells					1026:1030	relevant skin cells	1012:1030	relevant skin cells	1012:1030	Dissolution studies using simulated body fluid and cell biology tests using relevant skin cells further proved the excellent bioactivity and positive effects of the foams on cell proliferation and migration.
32268322	6	47	theme	cell	1110:1113	arg1	proliferation					1115:1127	cell proliferation	1110:1127	cell proliferation	1110:1127	Dissolution studies using simulated body fluid and cell biology tests using relevant skin cells further proved the excellent bioactivity and positive effects of the foams on cell proliferation and migration.
32268322	3	48	theme	favorable	523:531	arg1	properties					580:589	antibacterial properties	566:589	antibacterial properties	566:589	Borate bioactive glasses are of great interest in wound-healing applications due to a combination of favorable features, such as angiogenic and antibacterial properties.
32268322	3	48	theme	favorable	523:531	arg1	features					533:540	favorable features	523:540	favorable features	523:540	Borate bioactive glasses are of great interest in wound-healing applications due to a combination of favorable features, such as angiogenic and antibacterial properties.
32268322	3	48	theme	favorable	523:531	arg1	angiogenic					551:560	angiogenic	551:560	angiogenic	551:560	Borate bioactive glasses are of great interest in wound-healing applications due to a combination of favorable features, such as angiogenic and antibacterial properties.
32268322	0	49	theme	methylcellulose	40:54	arg1	foams					56:60	methylcellulose foams	40:60	methylcellulose foams with antibacterial effects for wound-healing applications	40:118	Manuka honey and bioactive glass impart methylcellulose foams with antibacterial effects for wound-healing applications.
32268322	6	50	theme	relevant	1012:1019	arg1	cells					1026:1030	relevant skin cells	1012:1030	relevant skin cells	1012:1030	Dissolution studies using simulated body fluid and cell biology tests using relevant skin cells further proved the excellent bioactivity and positive effects of the foams on cell proliferation and migration.
32268322	1	51	theme	able	137:140	arg1	dressings					127:135	Wound dressings	121:135	Wound dressings able to deliver topically bioactive molecules	121:181	Wound dressings able to deliver topically bioactive molecules represent a new generation of wound-regeneration therapies.
32268322	2	52	used	used	327:330	arg2	platform					337:344	a platform to deliver borate bioactive glass particles doped additionally with copper	335:419	a platform to deliver borate bioactive glass particles doped additionally with copper	335:419	In this article, foams based on methylcellulose cross-linked with Manuka honey were used as a platform to deliver borate bioactive glass particles doped additionally with copper.
32268322	2	52	used	used	327:330	arg2	foams					260:264	foams	260:264	foams based on methylcellulose cross-linked with Manuka honey	260:320	In this article, foams based on methylcellulose cross-linked with Manuka honey were used as a platform to deliver borate bioactive glass particles doped additionally with copper.
32268322	4	53	theme	bioactive	655:663	arg1	glass					665:669	the bioactive glass and Manuka honey	651:686	glass	665:669	The multifunctional composite providing the dual effect of the bioactive glass and Manuka honey was produced by freeze-drying, and the resulting foams exhibit suitable morphology characterized by high porosity.
32268322	8	54	theme	wound	1392:1396	arg1	outcomes					1370:1377	promising outcomes	1360:1377	promising outcomes	1360:1377	Therefore, the multifunctional foams showed promising outcomes as potential wound dressings for the treatment of infected wounds.
32268322	8	54	theme	wound	1392:1396	arg1	dressings					1398:1406	potential wound dressings	1382:1406	potential wound dressings for the treatment of infected wounds	1382:1443	Therefore, the multifunctional foams showed promising outcomes as potential wound dressings for the treatment of infected wounds.
32268322	0	55	with	foams	56:60	arg1	effects					81:87	antibacterial effects	67:87	antibacterial effects	67:87	Manuka honey and bioactive glass impart methylcellulose foams with antibacterial effects for wound-healing applications.
32268322	8	56	theme	potential	1382:1390	arg1	outcomes					1370:1377	promising outcomes	1360:1377	promising outcomes	1360:1377	Therefore, the multifunctional foams showed promising outcomes as potential wound dressings for the treatment of infected wounds.
32268322	8	56	theme	potential	1382:1390	arg1	dressings					1398:1406	potential wound dressings	1382:1406	potential wound dressings for the treatment of infected wounds	1382:1443	Therefore, the multifunctional foams showed promising outcomes as potential wound dressings for the treatment of infected wounds.
32268322	6	57	theme	foams	1101:1105	arg1	bioactivity					1061:1071	the excellent bioactivity	1047:1071	the excellent bioactivity	1047:1071	Dissolution studies using simulated body fluid and cell biology tests using relevant skin cells further proved the excellent bioactivity and positive effects of the foams on cell proliferation and migration.
32268322	6	57	theme	foams	1101:1105	arg1	effects					1086:1092	positive effects	1077:1092	positive effects of the foams on cell proliferation and migration	1077:1141	Dissolution studies using simulated body fluid and cell biology tests using relevant skin cells further proved the excellent bioactivity and positive effects of the foams on cell proliferation and migration.
32268322	0	58	theme	antibacterial	67:79	arg1	effects					81:87	antibacterial effects	67:87	antibacterial effects	67:87	Manuka honey and bioactive glass impart methylcellulose foams with antibacterial effects for wound-healing applications.
32268322	5	59	theme	glass	919:923	arg1	particles					925:933	bioactive glass particles	909:933	bioactive glass particles	909:933	Moreover, the performed tests showed improved wettability and mechanical performance with the addition of bioactive glass particles.
32268322	8	60	theme	multifunctional	1331:1345	arg1	foams					1347:1351	the multifunctional foams	1327:1351	the multifunctional foams	1327:1351	Therefore, the multifunctional foams showed promising outcomes as potential wound dressings for the treatment of infected wounds.
32268322	3	61	from	interest	460:467	arg1	applications					486:497	wound-healing applications	472:497	wound-healing applications due to a combination of favorable features, such as angiogenic and antibacterial properties	472:589	Borate bioactive glasses are of great interest in wound-healing applications due to a combination of favorable features, such as angiogenic and antibacterial properties.
32268322	4	62	theme	resulting	727:735	arg1	foams					737:741	the resulting foams	723:741	the resulting foams	723:741	The multifunctional composite providing the dual effect of the bioactive glass and Manuka honey was produced by freeze-drying, and the resulting foams exhibit suitable morphology characterized by high porosity.
32268322	3	63	theme	wound-healing	472:484	arg1	applications					486:497	wound-healing applications	472:497	wound-healing applications due to a combination of favorable features, such as angiogenic and antibacterial properties	472:589	Borate bioactive glasses are of great interest in wound-healing applications due to a combination of favorable features, such as angiogenic and antibacterial properties.
32268322	7	64	theme	ions	1204:1207	arg1	release					1176:1182	the dual release	1167:1182	the dual release of Manuka honey and ions from the copper-doped bioactive glass	1167:1245	Most interestingly, by the dual release of Manuka honey and ions from the copper-doped bioactive glass, an antibacterial effect against E. coli and S. aureus was achieved.
32268322	4	65	theme	honey	682:686	arg1	effect					641:646	the dual effect	632:646	the dual effect of the bioactive glass and Manuka honey	632:686	The multifunctional composite providing the dual effect of the bioactive glass and Manuka honey was produced by freeze-drying, and the resulting foams exhibit suitable morphology characterized by high porosity.
32268322	1	66	theme	bioactive	163:171	arg1	molecules					173:181	topically bioactive molecules	153:181	topically bioactive molecules	153:181	Wound dressings able to deliver topically bioactive molecules represent a new generation of wound-regeneration therapies.
32268322	3	67	theme	features	533:540	arg1	combination					508:518	a combination	506:518	a combination of favorable features, such as angiogenic and antibacterial properties	506:589	Borate bioactive glasses are of great interest in wound-healing applications due to a combination of favorable features, such as angiogenic and antibacterial properties.
32268322	4	68	theme	glass	665:669	arg1	effect					641:646	the dual effect	632:646	the dual effect of the bioactive glass and Manuka honey	632:686	The multifunctional composite providing the dual effect of the bioactive glass and Manuka honey was produced by freeze-drying, and the resulting foams exhibit suitable morphology characterized by high porosity.
32857867	4	0	theme	breads	777:782	arg1	value					768:772	the nutritional value	752:772	the nutritional value of breads, especially the β-glucan contents of waxy HLB breads	752:835	The addition of a high proportion of HLB WGFs significantly increased the nutritional value of breads, especially the β-glucan contents of waxy HLB breads.
32857867	11	1	theme	sensory	1892:1898	arg1	quality					1900:1906	sensory quality	1892:1906	sensory quality	1892:1906	However, high β-glucan and total dietary fiber was also accompanied by a negative effect on the sensory quality and processing performance of the end product.
32857867	10	2	theme	positive	1730:1737	arg1	effect					1739:1744	a positive effect	1728:1744	a positive effect	1728:1744	Higher β-glucan and total dietary fiber content in HLB might have a positive effect on the nutritional value of the resultant breads.
32857867	8	3	theme	V	1536:1536	arg1	type					1538:1541	A to V type	1531:1541	A to V type	1531:1541	After baking, the starch crystallinity of dough changed from A to V type, and the relative crystallinity decreased.
32857867	1	4	theme	Hull-less	227:235	arg1	HLB					267:269	waxy HLB	262:269	especially waxy HLB	251:269	Hull-less barley (HLB), especially waxy HLB, contains many physiologically active ingredients; however, its poor processing performance and end-product quality are unfavorable.
32857867	1	4	theme	Hull-less	227:235	arg1	HLB					245:247	HLB	245:247	HLB	245:247	Hull-less barley (HLB), especially waxy HLB, contains many physiologically active ingredients; however, its poor processing performance and end-product quality are unfavorable.
32857867	1	4	theme	Hull-less	227:235	arg1	barley					237:242	Hull-less barley	227:242	Hull-less barley (HLB)	227:248	Hull-less barley (HLB), especially waxy HLB, contains many physiologically active ingredients; however, its poor processing performance and end-product quality are unfavorable.
32857867	8	5	theme	starch	1488:1493	arg1	crystallinity					1495:1507	the starch crystallinity	1484:1507	the starch crystallinity of dough	1484:1516	After baking, the starch crystallinity of dough changed from A to V type, and the relative crystallinity decreased.
32857867	0	6	theme	textural	159:166	arg1	qualities					168:176	textural qualities	159:176	textural qualities	159:176	Effects of the addition of waxy and normal hull-less barley flours on the farinograph and pasting properties of composite flours and on the nutritional value, textural qualities, and in vitro digestibility of resultant breads.
32857867	11	7	theme	dietary	1829:1835	arg1	fiber					1837:1841	total dietary fiber	1823:1841	total dietary fiber	1823:1841	However, high β-glucan and total dietary fiber was also accompanied by a negative effect on the sensory quality and processing performance of the end product.
32857867	5	8	theme	farinograph	1078:1088	arg1	number					1098:1103	farinograph quality number	1078:1103	farinograph quality number	1078:1103	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	11	9	theme	product	1946:1952	arg1	performance					1923:1933	processing performance	1912:1933	processing performance	1912:1933	However, high β-glucan and total dietary fiber was also accompanied by a negative effect on the sensory quality and processing performance of the end product.
32857867	11	9	theme	product	1946:1952	arg1	quality					1900:1906	sensory quality	1892:1906	sensory quality	1892:1906	However, high β-glucan and total dietary fiber was also accompanied by a negative effect on the sensory quality and processing performance of the end product.
32857867	11	10	dep	quality	1900:1906	arg1	the					1888:1890	the	1888:1890	the	1888:1890	However, high β-glucan and total dietary fiber was also accompanied by a negative effect on the sensory quality and processing performance of the end product.
32857867	0	11	dep	in	183:184	arg1	vitro					186:190	vitro	186:190	vitro	186:190	Effects of the addition of waxy and normal hull-less barley flours on the farinograph and pasting properties of composite flours and on the nutritional value, textural qualities, and in vitro digestibility of resultant breads.
32857867	3	12	theme	breads	659:664	arg1	hydrolysis					635:644	in vitro hydrolysis	626:644	in vitro hydrolysis of resultant breads	626:664	The farinograph and pasting properties of composite powders, and the nutritional value, textural properties, and in vitro hydrolysis of resultant breads were evaluated.
32857867	3	12	theme	breads	659:664	arg1	properties					610:619	textural properties	601:619	textural properties	601:619	The farinograph and pasting properties of composite powders, and the nutritional value, textural properties, and in vitro hydrolysis of resultant breads were evaluated.
32857867	3	12	theme	breads	659:664	arg1	value					594:598	the nutritional value	578:598	the nutritional value	578:598	The farinograph and pasting properties of composite powders, and the nutritional value, textural properties, and in vitro hydrolysis of resultant breads were evaluated.
32857867	3	12	theme	breads	659:664	arg1	properties					541:550	The farinograph and pasting properties	513:550	The farinograph and pasting properties of composite powders	513:571	The farinograph and pasting properties of composite powders, and the nutritional value, textural properties, and in vitro hydrolysis of resultant breads were evaluated.
32857867	0	13	theme	in	183:184	arg1	digestibility					192:204	in vitro digestibility	183:204	in vitro digestibility	183:204	Effects of the addition of waxy and normal hull-less barley flours on the farinograph and pasting properties of composite flours and on the nutritional value, textural qualities, and in vitro digestibility of resultant breads.
32857867	2	14	theme	wheat	473:477	arg1	flour					479:483	20% wheat flour	469:483	20% wheat flour	469:483	In this study, 80% waxy or normal HLB wholegrain flour (WGF) and 20% wheat flour were used for baking bread.
32857867	5	15	theme	HLB	939:941	arg1	flours					949:954	HLB bread flours	939:954	HLB bread flours	939:954	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	2	16	theme	20	469:470	arg1	%					471:471	%	471:471	%	471:471	In this study, 80% waxy or normal HLB wholegrain flour (WGF) and 20% wheat flour were used for baking bread.
32857867	6	17	theme	wheat	1188:1192	arg1	bread					1194:1198	wheat bread	1188:1198	wheat bread	1188:1198	Although the sensory profiles of HLB breads were considerably lower than those of wheat bread, they still received a good overall acceptability from a panel of sensory evaluators.
32857867	5	18	theme	flours	949:954	arg1	softening					926:934	softening	926:934	softening of HLB bread flours	926:954	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	4	19	theme	β-glucan	800:807	arg1	contents					809:816	the β-glucan contents	796:816	the β-glucan contents of waxy HLB breads	796:835	The addition of a high proportion of HLB WGFs significantly increased the nutritional value of breads, especially the β-glucan contents of waxy HLB breads.
32857867	4	19	theme	β-glucan	800:807	arg1	breads					777:782	breads	777:782	breads	777:782	The addition of a high proportion of HLB WGFs significantly increased the nutritional value of breads, especially the β-glucan contents of waxy HLB breads.
32857867	1	20	theme	end-product	367:377	arg1	quality					379:385	end-product quality	367:385	end-product quality	367:385	Hull-less barley (HLB), especially waxy HLB, contains many physiologically active ingredients; however, its poor processing performance and end-product quality are unfavorable.
32857867	5	21	theme	softening	926:934	arg1	degree					916:921	a lower degree	908:921	a lower degree of softening of HLB bread flours	908:954	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	10	22	theme	Higher	1662:1667	arg1	β-glucan					1669:1676	Higher β-glucan	1662:1676	Higher β-glucan	1662:1676	Higher β-glucan and total dietary fiber content in HLB might have a positive effect on the nutritional value of the resultant breads.
32857867	11	23	theme	processing	1912:1921	arg1	performance					1923:1933	processing performance	1912:1933	processing performance	1912:1933	However, high β-glucan and total dietary fiber was also accompanied by a negative effect on the sensory quality and processing performance of the end product.
32857867	2	24	theme	wholegrain	442:451	arg1	flour					453:457	normal HLB wholegrain flour	431:457	normal HLB wholegrain flour	431:457	In this study, 80% waxy or normal HLB wholegrain flour (WGF) and 20% wheat flour were used for baking bread.
32857867	2	25	used	used	490:493	arg2	waxy					423:426	80% waxy	419:426	80% waxy	419:426	In this study, 80% waxy or normal HLB wholegrain flour (WGF) and 20% wheat flour were used for baking bread.
32857867	2	25	used	used	490:493	arg2	WGF					460:462	WGF	460:462	WGF	460:462	In this study, 80% waxy or normal HLB wholegrain flour (WGF) and 20% wheat flour were used for baking bread.
32857867	2	25	used	used	490:493	arg2	flour					479:483	20% wheat flour	469:483	20% wheat flour	469:483	In this study, 80% waxy or normal HLB wholegrain flour (WGF) and 20% wheat flour were used for baking bread.
32857867	2	25	used	used	490:493	arg2	flour					453:457	normal HLB wholegrain flour	431:457	normal HLB wholegrain flour	431:457	In this study, 80% waxy or normal HLB wholegrain flour (WGF) and 20% wheat flour were used for baking bread.
32857867	0	26	theme	hull-less	43:51	arg1	flours					60:65	normal hull-less barley flours	36:65	normal hull-less barley flours	36:65	Effects of the addition of waxy and normal hull-less barley flours on the farinograph and pasting properties of composite flours and on the nutritional value, textural qualities, and in vitro digestibility of resultant breads.
32857867	1	27	theme	many	281:284	arg1	ingredients					309:319	many physiologically active ingredients	281:319	many physiologically active ingredients	281:319	Hull-less barley (HLB), especially waxy HLB, contains many physiologically active ingredients; however, its poor processing performance and end-product quality are unfavorable.
32857867	7	28	theme	equilibrium	1422:1432	arg1	concentration					1434:1446	equilibrium concentration	1422:1446	equilibrium concentration	1422:1446	HLB breads, particularly the waxy types, exhibited higher hardness, gumminess, chewiness, and lower specific volume, glycemic index and equilibrium concentration in starch hydrolysis.
32857867	2	29	theme	normal	431:436	arg1	flour					453:457	normal HLB wholegrain flour	431:457	normal HLB wholegrain flour	431:457	In this study, 80% waxy or normal HLB wholegrain flour (WGF) and 20% wheat flour were used for baking bread.
32857867	9	30	theme	HLB	1600:1602	arg1	breads					1604:1609	waxy HLB breads	1595:1609	waxy HLB breads	1595:1609	Overall, waxy HLB breads had more nutritional value than normal HLB breads.
32857867	0	31	theme	flours	60:65	arg1	addition					15:22	the addition	11:22	the addition of waxy and normal hull-less barley flours	11:65	Effects of the addition of waxy and normal hull-less barley flours on the farinograph and pasting properties of composite flours and on the nutritional value, textural qualities, and in vitro digestibility of resultant breads.
32857867	1	32	theme	active	302:307	arg1	ingredients					309:319	many physiologically active ingredients	281:319	many physiologically active ingredients	281:319	Hull-less barley (HLB), especially waxy HLB, contains many physiologically active ingredients; however, its poor processing performance and end-product quality are unfavorable.
32857867	6	33	theme	HLB	1139:1141	arg1	breads					1143:1148	HLB breads	1139:1148	HLB breads	1139:1148	Although the sensory profiles of HLB breads were considerably lower than those of wheat bread, they still received a good overall acceptability from a panel of sensory evaluators.
32857867	10	34	theme	fiber	1696:1700	arg1	content					1702:1708	total dietary fiber content	1682:1708	total dietary fiber content	1682:1708	Higher β-glucan and total dietary fiber content in HLB might have a positive effect on the nutritional value of the resultant breads.
32857867	0	35	from	Effects	0:6	arg1	properties					98:107	the farinograph and pasting properties	70:107	the farinograph and pasting properties of composite flours	70:127	Effects of the addition of waxy and normal hull-less barley flours on the farinograph and pasting properties of composite flours and on the nutritional value, textural qualities, and in vitro digestibility of resultant breads.
32857867	0	35	from	Effects	0:6	arg1	qualities					168:176	textural qualities	159:176	textural qualities	159:176	Effects of the addition of waxy and normal hull-less barley flours on the farinograph and pasting properties of composite flours and on the nutritional value, textural qualities, and in vitro digestibility of resultant breads.
32857867	0	35	from	Effects	0:6	arg1	digestibility					192:204	in vitro digestibility	183:204	in vitro digestibility	183:204	Effects of the addition of waxy and normal hull-less barley flours on the farinograph and pasting properties of composite flours and on the nutritional value, textural qualities, and in vitro digestibility of resultant breads.
32857867	0	35	from	Effects	0:6	arg1	value					152:156	the nutritional value	136:156	the nutritional value	136:156	Effects of the addition of waxy and normal hull-less barley flours on the farinograph and pasting properties of composite flours and on the nutritional value, textural qualities, and in vitro digestibility of resultant breads.
32857867	12	36	theme	20 g	2043:2046	arg1	flour					2054:2058	20 g wheat flour	2043:2058	20 g wheat flour	2043:2058	PRACTICAL APPLICATION: The composite flour with 80 g hull-less barley wholegrain flour, 20 g wheat flour, and 30 g wheat gluten can be substituted in breadmaking.
32857867	13	37	theme	more	2228:2231	arg1	value					2245:2249	more nutritional value	2228:2249	more nutritional value	2228:2249	Compared to wheat bread, hull-less barley bread exhibited different but acceptable sensory properties and had more nutritional value, particularly the waxy one.
32857867	0	38	theme	breads	219:224	arg1	value					152:156	the nutritional value	136:156	the nutritional value	136:156	Effects of the addition of waxy and normal hull-less barley flours on the farinograph and pasting properties of composite flours and on the nutritional value, textural qualities, and in vitro digestibility of resultant breads.
32857867	0	38	theme	breads	219:224	arg1	qualities					168:176	textural qualities	159:176	textural qualities	159:176	Effects of the addition of waxy and normal hull-less barley flours on the farinograph and pasting properties of composite flours and on the nutritional value, textural qualities, and in vitro digestibility of resultant breads.
32857867	0	38	theme	breads	219:224	arg1	digestibility					192:204	in vitro digestibility	183:204	in vitro digestibility	183:204	Effects of the addition of waxy and normal hull-less barley flours on the farinograph and pasting properties of composite flours and on the nutritional value, textural qualities, and in vitro digestibility of resultant breads.
32857867	14	39	theme	bread	2353:2357	arg1	production					2359:2368	bread production	2353:2368	bread production	2353:2368	Therefore, a high proportion of hull-less barley could be recommended for bread production.
32857867	9	40	theme	more	1615:1618	arg1	value					1632:1636	more nutritional value	1615:1636	more nutritional value	1615:1636	Overall, waxy HLB breads had more nutritional value than normal HLB breads.
32857867	5	41	theme	farinograph	973:983	arg1	rate					1034:1037	higher water absorption rate	1010:1037	higher water absorption rate	1010:1037	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	5	41	theme	farinograph	973:983	arg1	time					1052:1055	development time	1040:1055	development time	1040:1055	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	5	41	theme	farinograph	973:983	arg1	time					1068:1071	stability time	1058:1071	stability time	1058:1071	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	5	41	theme	farinograph	973:983	arg1	characteristics					985:999	its farinograph characteristics	969:999	its farinograph characteristics	969:999	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	5	41	theme	farinograph	973:983	arg1	number					1098:1103	farinograph quality number	1078:1103	farinograph quality number	1078:1103	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	0	42	theme	waxy	27:30	arg1	addition					15:22	the addition	11:22	the addition of waxy and normal hull-less barley flours	11:65	Effects of the addition of waxy and normal hull-less barley flours on the farinograph and pasting properties of composite flours and on the nutritional value, textural qualities, and in vitro digestibility of resultant breads.
32857867	5	43	theme	suitable	869:876	arg1	amount					878:883	a suitable amount	867:883	a suitable amount of wheat gluten	867:899	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	5	43	theme	suitable	869:876	arg1	gluten					894:899	wheat gluten	888:899	wheat gluten	888:899	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	5	43	theme	suitable	869:876	arg1	WGFs					858:861	HLB WGFs	854:861	HLB WGFs	854:861	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	12	44	theme	30 g	2065:2068	arg1	gluten					2076:2081	30 g wheat gluten	2065:2081	30 g wheat gluten	2065:2081	PRACTICAL APPLICATION: The composite flour with 80 g hull-less barley wholegrain flour, 20 g wheat flour, and 30 g wheat gluten can be substituted in breadmaking.
32857867	12	45	theme	barley	2018:2023	arg1	flour					2036:2040	80 g hull-less barley wholegrain flour	2003:2040	80 g hull-less barley wholegrain flour	2003:2040	PRACTICAL APPLICATION: The composite flour with 80 g hull-less barley wholegrain flour, 20 g wheat flour, and 30 g wheat gluten can be substituted in breadmaking.
32857867	5	46	theme	development	1040:1050	arg1	time					1052:1055	development time	1040:1055	development time	1040:1055	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	9	47	theme	normal	1643:1648	arg1	breads					1654:1659	normal HLB breads	1643:1659	normal HLB breads	1643:1659	Overall, waxy HLB breads had more nutritional value than normal HLB breads.
32857867	8	48	dep	type	1538:1541	arg1	to					1533:1534	to	1533:1534	to	1533:1534	After baking, the starch crystallinity of dough changed from A to V type, and the relative crystallinity decreased.
32857867	6	49	theme	overall	1228:1234	arg1	acceptability					1236:1248	a good overall acceptability	1221:1248	a good overall acceptability from a panel of sensory evaluators	1221:1283	Although the sensory profiles of HLB breads were considerably lower than those of wheat bread, they still received a good overall acceptability from a panel of sensory evaluators.
32857867	7	50	theme	lower	1380:1384	arg1	volume					1395:1400	lower specific volume	1380:1400	lower specific volume	1380:1400	HLB breads, particularly the waxy types, exhibited higher hardness, gumminess, chewiness, and lower specific volume, glycemic index and equilibrium concentration in starch hydrolysis.
32857867	5	51	theme	HLB	854:856	arg1	WGFs					858:861	HLB WGFs	854:861	HLB WGFs	854:861	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	10	52	from	β-glucan	1669:1676	arg1	HLB					1713:1715	HLB	1713:1715	HLB	1713:1715	Higher β-glucan and total dietary fiber content in HLB might have a positive effect on the nutritional value of the resultant breads.
32857867	10	53	theme	dietary	1688:1694	arg1	content					1702:1708	total dietary fiber content	1682:1708	total dietary fiber content	1682:1708	Higher β-glucan and total dietary fiber content in HLB might have a positive effect on the nutritional value of the resultant breads.
32857867	4	54	theme	HLB	719:721	arg1	WGFs					723:726	HLB WGFs	719:726	HLB WGFs	719:726	The addition of a high proportion of HLB WGFs significantly increased the nutritional value of breads, especially the β-glucan contents of waxy HLB breads.
32857867	6	55	theme	sensory	1266:1272	arg1	evaluators					1274:1283	sensory evaluators	1266:1283	sensory evaluators	1266:1283	Although the sensory profiles of HLB breads were considerably lower than those of wheat bread, they still received a good overall acceptability from a panel of sensory evaluators.
32857867	1	56	theme	waxy	262:265	arg1	HLB					267:269	waxy HLB	262:269	especially waxy HLB	251:269	Hull-less barley (HLB), especially waxy HLB, contains many physiologically active ingredients; however, its poor processing performance and end-product quality are unfavorable.
32857867	1	56	theme	waxy	262:265	arg1	barley					237:242	Hull-less barley	227:242	Hull-less barley (HLB)	227:248	Hull-less barley (HLB), especially waxy HLB, contains many physiologically active ingredients; however, its poor processing performance and end-product quality are unfavorable.
32857867	7	57	from	index	1412:1416	arg1	hydrolysis					1458:1467	starch hydrolysis	1451:1467	starch hydrolysis	1451:1467	HLB breads, particularly the waxy types, exhibited higher hardness, gumminess, chewiness, and lower specific volume, glycemic index and equilibrium concentration in starch hydrolysis.
32857867	2	58	theme	80	419:420	arg1	%					421:421	%	421:421	%	421:421	In this study, 80% waxy or normal HLB wholegrain flour (WGF) and 20% wheat flour were used for baking bread.
32857867	5	59	theme	water	1017:1021	arg1	rate					1034:1037	higher water absorption rate	1010:1037	higher water absorption rate	1010:1037	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	3	60	theme	composite	555:563	arg1	powders					565:571	composite powders	555:571	composite powders	555:571	The farinograph and pasting properties of composite powders, and the nutritional value, textural properties, and in vitro hydrolysis of resultant breads were evaluated.
32857867	12	61	theme	80 g	2003:2006	arg1	flour					2036:2040	80 g hull-less barley wholegrain flour	2003:2040	80 g hull-less barley wholegrain flour	2003:2040	PRACTICAL APPLICATION: The composite flour with 80 g hull-less barley wholegrain flour, 20 g wheat flour, and 30 g wheat gluten can be substituted in breadmaking.
32857867	11	62	theme	end	1942:1944	arg1	product					1946:1952	the end product	1938:1952	the end product	1938:1952	However, high β-glucan and total dietary fiber was also accompanied by a negative effect on the sensory quality and processing performance of the end product.
32857867	14	63	theme	barley	2321:2326	arg1	proportion					2297:2306	a high proportion	2290:2306	a high proportion of hull-less barley	2290:2326	Therefore, a high proportion of hull-less barley could be recommended for bread production.
32857867	5	64	theme	lower	910:914	arg1	degree					916:921	a lower degree	908:921	a lower degree of softening of HLB bread flours	908:954	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	5	65	theme	quality	1090:1096	arg1	number					1098:1103	farinograph quality number	1078:1103	farinograph quality number	1078:1103	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	13	66	theme	barley	2153:2158	arg1	bread					2160:2164	hull-less barley bread	2143:2164	hull-less barley bread	2143:2164	Compared to wheat bread, hull-less barley bread exhibited different but acceptable sensory properties and had more nutritional value, particularly the waxy one.
32857867	4	67	theme	proportion	705:714	arg1	addition					686:693	The addition	682:693	The addition of a high proportion of HLB WGFs	682:726	The addition of a high proportion of HLB WGFs significantly increased the nutritional value of breads, especially the β-glucan contents of waxy HLB breads.
32857867	8	68	theme	dough	1512:1516	arg1	crystallinity					1495:1507	the starch crystallinity	1484:1507	the starch crystallinity of dough	1484:1516	After baking, the starch crystallinity of dough changed from A to V type, and the relative crystallinity decreased.
32857867	7	69	from	gumminess	1354:1362	arg1	hydrolysis					1458:1467	starch hydrolysis	1451:1467	starch hydrolysis	1451:1467	HLB breads, particularly the waxy types, exhibited higher hardness, gumminess, chewiness, and lower specific volume, glycemic index and equilibrium concentration in starch hydrolysis.
32857867	7	70	theme	higher	1337:1342	arg1	hardness					1344:1351	higher hardness	1337:1351	higher hardness	1337:1351	HLB breads, particularly the waxy types, exhibited higher hardness, gumminess, chewiness, and lower specific volume, glycemic index and equilibrium concentration in starch hydrolysis.
32857867	5	71	theme	gluten	894:899	arg1	amount					878:883	a suitable amount	867:883	a suitable amount of wheat gluten	867:899	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	5	71	theme	gluten	894:899	arg1	gluten					894:899	wheat gluten	888:899	wheat gluten	888:899	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	5	71	theme	gluten	894:899	arg1	addition					842:849	The addition	838:849	The addition of HLB WGFs	838:861	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	5	71	theme	gluten	894:899	arg1	WGFs					858:861	HLB WGFs	854:861	HLB WGFs	854:861	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	5	72	theme	absorption	1023:1032	arg1	rate					1034:1037	higher water absorption rate	1010:1037	higher water absorption rate	1010:1037	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	9	73	theme	waxy	1595:1598	arg1	breads					1604:1609	waxy HLB breads	1595:1609	waxy HLB breads	1595:1609	Overall, waxy HLB breads had more nutritional value than normal HLB breads.
32857867	7	74	from	chewiness	1365:1373	arg1	hydrolysis					1458:1467	starch hydrolysis	1451:1467	starch hydrolysis	1451:1467	HLB breads, particularly the waxy types, exhibited higher hardness, gumminess, chewiness, and lower specific volume, glycemic index and equilibrium concentration in starch hydrolysis.
32857867	3	75	theme	textural	601:608	arg1	properties					610:619	textural properties	601:619	textural properties	601:619	The farinograph and pasting properties of composite powders, and the nutritional value, textural properties, and in vitro hydrolysis of resultant breads were evaluated.
32857867	6	76	theme	breads	1143:1148	arg1	profiles					1127:1134	the sensory profiles	1115:1134	the sensory profiles of HLB breads	1115:1148	Although the sensory profiles of HLB breads were considerably lower than those of wheat bread, they still received a good overall acceptability from a panel of sensory evaluators.
32857867	6	76	theme	breads	1143:1148	arg1	lower					1168:1172	lower	1168:1172	lower	1168:1172	Although the sensory profiles of HLB breads were considerably lower than those of wheat bread, they still received a good overall acceptability from a panel of sensory evaluators.
32857867	11	77	theme	total	1823:1827	arg1	fiber					1837:1841	total dietary fiber	1823:1841	total dietary fiber	1823:1841	However, high β-glucan and total dietary fiber was also accompanied by a negative effect on the sensory quality and processing performance of the end product.
32857867	10	78	from	content	1702:1708	arg1	HLB					1713:1715	HLB	1713:1715	HLB	1713:1715	Higher β-glucan and total dietary fiber content in HLB might have a positive effect on the nutritional value of the resultant breads.
32857867	7	79	theme	waxy	1315:1318	arg1	types					1320:1324	the waxy types	1311:1324	particularly the waxy types	1298:1324	HLB breads, particularly the waxy types, exhibited higher hardness, gumminess, chewiness, and lower specific volume, glycemic index and equilibrium concentration in starch hydrolysis.
32857867	7	79	theme	waxy	1315:1318	arg1	breads					1290:1295	HLB breads	1286:1295	HLB breads	1286:1295	HLB breads, particularly the waxy types, exhibited higher hardness, gumminess, chewiness, and lower specific volume, glycemic index and equilibrium concentration in starch hydrolysis.
32857867	2	80	theme	baking	499:504	arg1	bread					506:510	baking bread	499:510	baking bread	499:510	In this study, 80% waxy or normal HLB wholegrain flour (WGF) and 20% wheat flour were used for baking bread.
32857867	10	81	contain	have	1723:1726	arg2	effect					1739:1744	a positive effect	1728:1744	a positive effect	1728:1744	Higher β-glucan and total dietary fiber content in HLB might have a positive effect on the nutritional value of the resultant breads.
32857867	10	81	contain	have	1723:1726	arg1	content					1702:1708	total dietary fiber content	1682:1708	total dietary fiber content	1682:1708	Higher β-glucan and total dietary fiber content in HLB might have a positive effect on the nutritional value of the resultant breads.
32857867	10	81	contain	have	1723:1726	arg1	β-glucan					1669:1676	Higher β-glucan	1662:1676	Higher β-glucan	1662:1676	Higher β-glucan and total dietary fiber content in HLB might have a positive effect on the nutritional value of the resultant breads.
32857867	10	82	theme	nutritional	1753:1763	arg1	value					1765:1769	the nutritional value	1749:1769	the nutritional value of the resultant breads	1749:1793	Higher β-glucan and total dietary fiber content in HLB might have a positive effect on the nutritional value of the resultant breads.
32857867	7	83	theme	HLB	1286:1288	arg1	types					1320:1324	the waxy types	1311:1324	particularly the waxy types	1298:1324	HLB breads, particularly the waxy types, exhibited higher hardness, gumminess, chewiness, and lower specific volume, glycemic index and equilibrium concentration in starch hydrolysis.
32857867	7	83	theme	HLB	1286:1288	arg1	breads					1290:1295	HLB breads	1286:1295	HLB breads	1286:1295	HLB breads, particularly the waxy types, exhibited higher hardness, gumminess, chewiness, and lower specific volume, glycemic index and equilibrium concentration in starch hydrolysis.
32857867	3	84	theme	in	626:627	arg1	hydrolysis					635:644	in vitro hydrolysis	626:644	in vitro hydrolysis of resultant breads	626:664	The farinograph and pasting properties of composite powders, and the nutritional value, textural properties, and in vitro hydrolysis of resultant breads were evaluated.
32857867	6	85	theme	sensory	1119:1125	arg1	profiles					1127:1134	the sensory profiles	1115:1134	the sensory profiles of HLB breads	1115:1148	Although the sensory profiles of HLB breads were considerably lower than those of wheat bread, they still received a good overall acceptability from a panel of sensory evaluators.
32857867	6	85	theme	sensory	1119:1125	arg1	lower					1168:1172	lower	1168:1172	lower	1168:1172	Although the sensory profiles of HLB breads were considerably lower than those of wheat bread, they still received a good overall acceptability from a panel of sensory evaluators.
32857867	10	86	theme	breads	1788:1793	arg1	value					1765:1769	the nutritional value	1749:1769	the nutritional value of the resultant breads	1749:1793	Higher β-glucan and total dietary fiber content in HLB might have a positive effect on the nutritional value of the resultant breads.
32857867	1	87	contain	contains	272:279	arg2	ingredients					309:319	many physiologically active ingredients	281:319	many physiologically active ingredients	281:319	Hull-less barley (HLB), especially waxy HLB, contains many physiologically active ingredients; however, its poor processing performance and end-product quality are unfavorable.
32857867	1	87	contain	contains	272:279	arg1	HLB					245:247	HLB	245:247	HLB	245:247	Hull-less barley (HLB), especially waxy HLB, contains many physiologically active ingredients; however, its poor processing performance and end-product quality are unfavorable.
32857867	1	87	contain	contains	272:279	arg1	barley					237:242	Hull-less barley	227:242	Hull-less barley (HLB)	227:248	Hull-less barley (HLB), especially waxy HLB, contains many physiologically active ingredients; however, its poor processing performance and end-product quality are unfavorable.
32857867	1	87	contain	contains	272:279	arg1	HLB					267:269	waxy HLB	262:269	especially waxy HLB	251:269	Hull-less barley (HLB), especially waxy HLB, contains many physiologically active ingredients; however, its poor processing performance and end-product quality are unfavorable.
32857867	12	88	dep	APPLICATION	1965:1975	arg1	substituted					2090:2100	substituted	2090:2100	can be substituted in breadmaking	2083:2115	PRACTICAL APPLICATION: The composite flour with 80 g hull-less barley wholegrain flour, 20 g wheat flour, and 30 g wheat gluten can be substituted in breadmaking.
32857867	4	89	theme	nutritional	756:766	arg1	value					768:772	the nutritional value	752:772	the nutritional value of breads, especially the β-glucan contents of waxy HLB breads	752:835	The addition of a high proportion of HLB WGFs significantly increased the nutritional value of breads, especially the β-glucan contents of waxy HLB breads.
32857867	10	90	theme	resultant	1778:1786	arg1	breads					1788:1793	the resultant breads	1774:1793	the resultant breads	1774:1793	Higher β-glucan and total dietary fiber content in HLB might have a positive effect on the nutritional value of the resultant breads.
32857867	13	91	theme	sensory	2201:2207	arg1	properties					2209:2218	different but acceptable sensory properties	2176:2218	different but acceptable sensory properties	2176:2218	Compared to wheat bread, hull-less barley bread exhibited different but acceptable sensory properties and had more nutritional value, particularly the waxy one.
32857867	9	92	contain	had	1611:1613	arg2	value					1632:1636	more nutritional value	1615:1636	more nutritional value	1615:1636	Overall, waxy HLB breads had more nutritional value than normal HLB breads.
32857867	9	92	contain	had	1611:1613	arg1	breads					1604:1609	waxy HLB breads	1595:1609	waxy HLB breads	1595:1609	Overall, waxy HLB breads had more nutritional value than normal HLB breads.
32857867	8	93	theme	relative	1552:1559	arg1	crystallinity					1561:1573	the relative crystallinity	1548:1573	the relative crystallinity	1548:1573	After baking, the starch crystallinity of dough changed from A to V type, and the relative crystallinity decreased.
32857867	4	94	theme	waxy	821:824	arg1	breads					830:835	waxy HLB breads	821:835	waxy HLB breads	821:835	The addition of a high proportion of HLB WGFs significantly increased the nutritional value of breads, especially the β-glucan contents of waxy HLB breads.
32857867	3	95	theme	resultant	649:657	arg1	breads					659:664	resultant breads	649:664	resultant breads	649:664	The farinograph and pasting properties of composite powders, and the nutritional value, textural properties, and in vitro hydrolysis of resultant breads were evaluated.
32857867	0	96	theme	flours	122:127	arg1	properties					98:107	the farinograph and pasting properties	70:107	the farinograph and pasting properties of composite flours	70:127	Effects of the addition of waxy and normal hull-less barley flours on the farinograph and pasting properties of composite flours and on the nutritional value, textural qualities, and in vitro digestibility of resultant breads.
32857867	5	97	theme	bread	943:947	arg1	flours					949:954	HLB bread flours	939:954	HLB bread flours	939:954	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	13	98	contain	had	2224:2226	arg1	bread					2160:2164	hull-less barley bread	2143:2164	hull-less barley bread	2143:2164	Compared to wheat bread, hull-less barley bread exhibited different but acceptable sensory properties and had more nutritional value, particularly the waxy one.
32857867	13	98	contain	had	2224:2226	arg2	value					2245:2249	more nutritional value	2228:2249	more nutritional value	2228:2249	Compared to wheat bread, hull-less barley bread exhibited different but acceptable sensory properties and had more nutritional value, particularly the waxy one.
32857867	4	99	theme	breads	830:835	arg1	contents					809:816	the β-glucan contents	796:816	the β-glucan contents of waxy HLB breads	796:835	The addition of a high proportion of HLB WGFs significantly increased the nutritional value of breads, especially the β-glucan contents of waxy HLB breads.
32857867	4	99	theme	breads	830:835	arg1	breads					777:782	breads	777:782	breads	777:782	The addition of a high proportion of HLB WGFs significantly increased the nutritional value of breads, especially the β-glucan contents of waxy HLB breads.
32857867	0	100	theme	farinograph	74:84	arg1	properties					98:107	the farinograph and pasting properties	70:107	the farinograph and pasting properties of composite flours	70:127	Effects of the addition of waxy and normal hull-less barley flours on the farinograph and pasting properties of composite flours and on the nutritional value, textural qualities, and in vitro digestibility of resultant breads.
32857867	7	101	from	hardness	1344:1351	arg1	hydrolysis					1458:1467	starch hydrolysis	1451:1467	starch hydrolysis	1451:1467	HLB breads, particularly the waxy types, exhibited higher hardness, gumminess, chewiness, and lower specific volume, glycemic index and equilibrium concentration in starch hydrolysis.
32857867	7	102	theme	starch	1451:1456	arg1	hydrolysis					1458:1467	starch hydrolysis	1451:1467	starch hydrolysis	1451:1467	HLB breads, particularly the waxy types, exhibited higher hardness, gumminess, chewiness, and lower specific volume, glycemic index and equilibrium concentration in starch hydrolysis.
32857867	4	103	theme	HLB	826:828	arg1	breads					830:835	waxy HLB breads	821:835	waxy HLB breads	821:835	The addition of a high proportion of HLB WGFs significantly increased the nutritional value of breads, especially the β-glucan contents of waxy HLB breads.
32857867	0	104	theme	nutritional	140:150	arg1	value					152:156	the nutritional value	136:156	the nutritional value	136:156	Effects of the addition of waxy and normal hull-less barley flours on the farinograph and pasting properties of composite flours and on the nutritional value, textural qualities, and in vitro digestibility of resultant breads.
32857867	6	105	from	panel	1257:1261	arg1	acceptability					1236:1248	a good overall acceptability	1221:1248	a good overall acceptability from a panel of sensory evaluators	1221:1283	Although the sensory profiles of HLB breads were considerably lower than those of wheat bread, they still received a good overall acceptability from a panel of sensory evaluators.
32857867	3	106	theme	pasting	533:539	arg1	properties					541:550	The farinograph and pasting properties	513:550	The farinograph and pasting properties of composite powders	513:571	The farinograph and pasting properties of composite powders, and the nutritional value, textural properties, and in vitro hydrolysis of resultant breads were evaluated.
32857867	13	107	theme	nutritional	2233:2243	arg1	value					2245:2249	more nutritional value	2228:2249	more nutritional value	2228:2249	Compared to wheat bread, hull-less barley bread exhibited different but acceptable sensory properties and had more nutritional value, particularly the waxy one.
32857867	0	108	theme	barley	53:58	arg1	flours					60:65	normal hull-less barley flours	36:65	normal hull-less barley flours	36:65	Effects of the addition of waxy and normal hull-less barley flours on the farinograph and pasting properties of composite flours and on the nutritional value, textural qualities, and in vitro digestibility of resultant breads.
32857867	11	109	theme	negative	1869:1876	arg1	effect					1878:1883	a negative effect	1867:1883	a negative effect on the sensory quality and processing performance of the end product	1867:1952	However, high β-glucan and total dietary fiber was also accompanied by a negative effect on the sensory quality and processing performance of the end product.
32857867	3	110	theme	farinograph	517:527	arg1	properties					541:550	The farinograph and pasting properties	513:550	The farinograph and pasting properties of composite powders	513:571	The farinograph and pasting properties of composite powders, and the nutritional value, textural properties, and in vitro hydrolysis of resultant breads were evaluated.
32857867	2	111	theme	HLB	438:440	arg1	flour					453:457	normal HLB wholegrain flour	431:457	normal HLB wholegrain flour	431:457	In this study, 80% waxy or normal HLB wholegrain flour (WGF) and 20% wheat flour were used for baking bread.
32857867	7	112	theme	glycemic	1403:1410	arg1	index					1412:1416	glycemic index	1403:1416	glycemic index	1403:1416	HLB breads, particularly the waxy types, exhibited higher hardness, gumminess, chewiness, and lower specific volume, glycemic index and equilibrium concentration in starch hydrolysis.
32857867	2	113	theme	%	471:471	arg1	flour					479:483	20% wheat flour	469:483	20% wheat flour	469:483	In this study, 80% waxy or normal HLB wholegrain flour (WGF) and 20% wheat flour were used for baking bread.
32857867	1	114	theme	poor	335:338	arg1	performance					351:361	its poor processing performance	331:361	its poor processing performance	331:361	Hull-less barley (HLB), especially waxy HLB, contains many physiologically active ingredients; however, its poor processing performance and end-product quality are unfavorable.
32857867	6	115	theme	good	1223:1226	arg1	acceptability					1236:1248	a good overall acceptability	1221:1248	a good overall acceptability from a panel of sensory evaluators	1221:1283	Although the sensory profiles of HLB breads were considerably lower than those of wheat bread, they still received a good overall acceptability from a panel of sensory evaluators.
32857867	5	116	theme	higher	1010:1015	arg1	rate					1034:1037	higher water absorption rate	1010:1037	higher water absorption rate	1010:1037	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	5	117	theme	WGFs	858:861	arg1	amount					878:883	a suitable amount	867:883	a suitable amount of wheat gluten	867:899	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	5	117	theme	WGFs	858:861	arg1	gluten					894:899	wheat gluten	888:899	wheat gluten	888:899	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	5	117	theme	WGFs	858:861	arg1	addition					842:849	The addition	838:849	The addition of HLB WGFs	838:861	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	5	117	theme	WGFs	858:861	arg1	WGFs					858:861	HLB WGFs	854:861	HLB WGFs	854:861	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	11	118	from	effect	1878:1883	arg1	performance					1923:1933	processing performance	1912:1933	processing performance	1912:1933	However, high β-glucan and total dietary fiber was also accompanied by a negative effect on the sensory quality and processing performance of the end product.
32857867	11	118	from	effect	1878:1883	arg1	quality					1900:1906	sensory quality	1892:1906	sensory quality	1892:1906	However, high β-glucan and total dietary fiber was also accompanied by a negative effect on the sensory quality and processing performance of the end product.
32857867	0	119	theme	pasting	90:96	arg1	properties					98:107	the farinograph and pasting properties	70:107	the farinograph and pasting properties of composite flours	70:127	Effects of the addition of waxy and normal hull-less barley flours on the farinograph and pasting properties of composite flours and on the nutritional value, textural qualities, and in vitro digestibility of resultant breads.
32857867	0	120	theme	resultant	209:217	arg1	breads					219:224	resultant breads	209:224	resultant breads	209:224	Effects of the addition of waxy and normal hull-less barley flours on the farinograph and pasting properties of composite flours and on the nutritional value, textural qualities, and in vitro digestibility of resultant breads.
32857867	9	121	theme	nutritional	1620:1630	arg1	value					1632:1636	more nutritional value	1615:1636	more nutritional value	1615:1636	Overall, waxy HLB breads had more nutritional value than normal HLB breads.
32857867	12	122	theme	wheat	2070:2074	arg1	gluten					2076:2081	30 g wheat gluten	2065:2081	30 g wheat gluten	2065:2081	PRACTICAL APPLICATION: The composite flour with 80 g hull-less barley wholegrain flour, 20 g wheat flour, and 30 g wheat gluten can be substituted in breadmaking.
32857867	12	123	theme	wheat	2048:2052	arg1	flour					2054:2058	20 g wheat flour	2043:2058	20 g wheat flour	2043:2058	PRACTICAL APPLICATION: The composite flour with 80 g hull-less barley wholegrain flour, 20 g wheat flour, and 30 g wheat gluten can be substituted in breadmaking.
32857867	12	124	with	flour	1992:1996	arg1	flour					2036:2040	80 g hull-less barley wholegrain flour	2003:2040	80 g hull-less barley wholegrain flour	2003:2040	PRACTICAL APPLICATION: The composite flour with 80 g hull-less barley wholegrain flour, 20 g wheat flour, and 30 g wheat gluten can be substituted in breadmaking.
32857867	12	124	with	flour	1992:1996	arg1	gluten					2076:2081	30 g wheat gluten	2065:2081	30 g wheat gluten	2065:2081	PRACTICAL APPLICATION: The composite flour with 80 g hull-less barley wholegrain flour, 20 g wheat flour, and 30 g wheat gluten can be substituted in breadmaking.
32857867	12	124	with	flour	1992:1996	arg1	flour					2054:2058	20 g wheat flour	2043:2058	20 g wheat flour	2043:2058	PRACTICAL APPLICATION: The composite flour with 80 g hull-less barley wholegrain flour, 20 g wheat flour, and 30 g wheat gluten can be substituted in breadmaking.
32857867	12	125	theme	hull-less	2008:2016	arg1	flour					2036:2040	80 g hull-less barley wholegrain flour	2003:2040	80 g hull-less barley wholegrain flour	2003:2040	PRACTICAL APPLICATION: The composite flour with 80 g hull-less barley wholegrain flour, 20 g wheat flour, and 30 g wheat gluten can be substituted in breadmaking.
32857867	1	126	theme	processing	340:349	arg1	performance					351:361	its poor processing performance	331:361	its poor processing performance	331:361	Hull-less barley (HLB), especially waxy HLB, contains many physiologically active ingredients; however, its poor processing performance and end-product quality are unfavorable.
32857867	0	127	theme	addition	15:22	arg1	Effects					0:6	Effects	0:6	Effects of the addition of waxy and normal hull-less barley flours on the farinograph and pasting properties of composite flours and on the nutritional value, textural qualities, and in vitro digestibility of resultant breads.	0:225	Effects of the addition of waxy and normal hull-less barley flours on the farinograph and pasting properties of composite flours and on the nutritional value, textural qualities, and in vitro digestibility of resultant breads.
32857867	7	128	from	volume	1395:1400	arg1	hydrolysis					1458:1467	starch hydrolysis	1451:1467	starch hydrolysis	1451:1467	HLB breads, particularly the waxy types, exhibited higher hardness, gumminess, chewiness, and lower specific volume, glycemic index and equilibrium concentration in starch hydrolysis.
32857867	9	129	theme	HLB	1650:1652	arg1	breads					1654:1659	normal HLB breads	1643:1659	normal HLB breads	1643:1659	Overall, waxy HLB breads had more nutritional value than normal HLB breads.
32857867	3	130	theme	powders	565:571	arg1	hydrolysis					635:644	in vitro hydrolysis	626:644	in vitro hydrolysis of resultant breads	626:664	The farinograph and pasting properties of composite powders, and the nutritional value, textural properties, and in vitro hydrolysis of resultant breads were evaluated.
32857867	3	130	theme	powders	565:571	arg1	properties					610:619	textural properties	601:619	textural properties	601:619	The farinograph and pasting properties of composite powders, and the nutritional value, textural properties, and in vitro hydrolysis of resultant breads were evaluated.
32857867	3	130	theme	powders	565:571	arg1	value					594:598	the nutritional value	578:598	the nutritional value	578:598	The farinograph and pasting properties of composite powders, and the nutritional value, textural properties, and in vitro hydrolysis of resultant breads were evaluated.
32857867	3	130	theme	powders	565:571	arg1	properties					541:550	The farinograph and pasting properties	513:550	The farinograph and pasting properties of composite powders	513:571	The farinograph and pasting properties of composite powders, and the nutritional value, textural properties, and in vitro hydrolysis of resultant breads were evaluated.
32857867	12	131	theme	wholegrain	2025:2034	arg1	flour					2036:2040	80 g hull-less barley wholegrain flour	2003:2040	80 g hull-less barley wholegrain flour	2003:2040	PRACTICAL APPLICATION: The composite flour with 80 g hull-less barley wholegrain flour, 20 g wheat flour, and 30 g wheat gluten can be substituted in breadmaking.
32857867	13	132	theme	wheat	2130:2134	arg1	bread					2136:2140	wheat bread	2130:2140	wheat bread	2130:2140	Compared to wheat bread, hull-less barley bread exhibited different but acceptable sensory properties and had more nutritional value, particularly the waxy one.
32857867	6	133	theme	evaluators	1274:1283	arg1	panel					1257:1261	a panel	1255:1261	a panel of sensory evaluators	1255:1283	Although the sensory profiles of HLB breads were considerably lower than those of wheat bread, they still received a good overall acceptability from a panel of sensory evaluators.
32857867	5	134	theme	stability	1058:1066	arg1	time					1068:1071	stability time	1058:1071	stability time	1058:1071	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	7	135	theme	specific	1386:1393	arg1	volume					1395:1400	lower specific volume	1380:1400	lower specific volume	1380:1400	HLB breads, particularly the waxy types, exhibited higher hardness, gumminess, chewiness, and lower specific volume, glycemic index and equilibrium concentration in starch hydrolysis.
32857867	13	136	theme	different	2176:2184	arg1	properties					2209:2218	different but acceptable sensory properties	2176:2218	different but acceptable sensory properties	2176:2218	Compared to wheat bread, hull-less barley bread exhibited different but acceptable sensory properties and had more nutritional value, particularly the waxy one.
32857867	2	137	theme	%	421:421	arg1	WGF					460:462	WGF	460:462	WGF	460:462	In this study, 80% waxy or normal HLB wholegrain flour (WGF) and 20% wheat flour were used for baking bread.
32857867	2	137	theme	%	421:421	arg1	waxy					423:426	80% waxy	419:426	80% waxy	419:426	In this study, 80% waxy or normal HLB wholegrain flour (WGF) and 20% wheat flour were used for baking bread.
32857867	10	138	theme	total	1682:1686	arg1	content					1702:1708	total dietary fiber content	1682:1708	total dietary fiber content	1682:1708	Higher β-glucan and total dietary fiber content in HLB might have a positive effect on the nutritional value of the resultant breads.
32857867	12	139	theme	composite	1982:1990	arg1	flour					1992:1996	The composite flour	1978:1996	The composite flour with 80 g hull-less barley wholegrain flour, 20 g wheat flour, and 30 g wheat gluten	1978:2081	PRACTICAL APPLICATION: The composite flour with 80 g hull-less barley wholegrain flour, 20 g wheat flour, and 30 g wheat gluten can be substituted in breadmaking.
32857867	0	140	theme	normal	36:41	arg1	flours					60:65	normal hull-less barley flours	36:65	normal hull-less barley flours	36:65	Effects of the addition of waxy and normal hull-less barley flours on the farinograph and pasting properties of composite flours and on the nutritional value, textural qualities, and in vitro digestibility of resultant breads.
32857867	3	141	dep	in	626:627	arg1	vitro					629:633	vitro	629:633	vitro	629:633	The farinograph and pasting properties of composite powders, and the nutritional value, textural properties, and in vitro hydrolysis of resultant breads were evaluated.
32857867	13	142	theme	acceptable	2190:2199	arg1	properties					2209:2218	different but acceptable sensory properties	2176:2218	different but acceptable sensory properties	2176:2218	Compared to wheat bread, hull-less barley bread exhibited different but acceptable sensory properties and had more nutritional value, particularly the waxy one.
32857867	0	143	theme	composite	112:120	arg1	flours					122:127	composite flours	112:127	composite flours	112:127	Effects of the addition of waxy and normal hull-less barley flours on the farinograph and pasting properties of composite flours and on the nutritional value, textural qualities, and in vitro digestibility of resultant breads.
32857867	14	144	theme	high	2292:2295	arg1	proportion					2297:2306	a high proportion	2290:2306	a high proportion of hull-less barley	2290:2326	Therefore, a high proportion of hull-less barley could be recommended for bread production.
32857867	3	145	theme	nutritional	582:592	arg1	value					594:598	the nutritional value	578:598	the nutritional value	578:598	The farinograph and pasting properties of composite powders, and the nutritional value, textural properties, and in vitro hydrolysis of resultant breads were evaluated.
32857867	4	146	theme	WGFs	723:726	arg1	proportion					705:714	a high proportion	698:714	a high proportion of HLB WGFs	698:726	The addition of a high proportion of HLB WGFs significantly increased the nutritional value of breads, especially the β-glucan contents of waxy HLB breads.
32857867	7	147	from	concentration	1434:1446	arg1	hydrolysis					1458:1467	starch hydrolysis	1451:1467	starch hydrolysis	1451:1467	HLB breads, particularly the waxy types, exhibited higher hardness, gumminess, chewiness, and lower specific volume, glycemic index and equilibrium concentration in starch hydrolysis.
32857867	14	148	theme	hull-less	2311:2319	arg1	barley					2321:2326	hull-less barley	2311:2326	hull-less barley	2311:2326	Therefore, a high proportion of hull-less barley could be recommended for bread production.
32857867	13	149	theme	hull-less	2143:2151	arg1	bread					2160:2164	hull-less barley bread	2143:2164	hull-less barley bread	2143:2164	Compared to wheat bread, hull-less barley bread exhibited different but acceptable sensory properties and had more nutritional value, particularly the waxy one.
32857867	12	150	theme	PRACTICAL	1955:1963	arg1	APPLICATION					1965:1975	PRACTICAL APPLICATION	1955:1975	PRACTICAL APPLICATION: The composite flour with 80 g hull-less barley wholegrain flour, 20 g wheat flour, and 30 g wheat gluten can be substituted in breadmaking.	1955:2116	PRACTICAL APPLICATION: The composite flour with 80 g hull-less barley wholegrain flour, 20 g wheat flour, and 30 g wheat gluten can be substituted in breadmaking.
32857867	11	151	theme	high	1805:1808	arg1	β-glucan					1810:1817	high β-glucan	1805:1817	high β-glucan	1805:1817	However, high β-glucan and total dietary fiber was also accompanied by a negative effect on the sensory quality and processing performance of the end product.
32857867	5	152	theme	wheat	888:892	arg1	gluten					894:899	wheat gluten	888:899	wheat gluten	888:899	The addition of HLB WGFs and a suitable amount of wheat gluten led to a lower degree of softening of HLB bread flours but improved its farinograph characteristics, such as higher water absorption rate, development time, stability time, and farinograph quality number.
32857867	4	153	theme	high	700:703	arg1	proportion					705:714	a high proportion	698:714	a high proportion of HLB WGFs	698:726	The addition of a high proportion of HLB WGFs significantly increased the nutritional value of breads, especially the β-glucan contents of waxy HLB breads.
32857867	8	154	theme	A	1531:1531	arg1	type					1538:1541	A to V type	1531:1541	A to V type	1531:1541	After baking, the starch crystallinity of dough changed from A to V type, and the relative crystallinity decreased.
32698067	4	0	contain	having	654:659	arg1	ALG1					649:652	ALG1	649:652	ALG1 having higher amount of G-blocks	649:685	ALG1 having higher amount of G-blocks was favourable in the complex formation with ATC.
32698067	4	0	contain	having	654:659	arg2	G-blocks					678:685	G-blocks	678:685	G-blocks	678:685	ALG1 having higher amount of G-blocks was favourable in the complex formation with ATC.
32698067	4	0	contain	having	654:659	arg2	amount					668:673	higher amount	661:673	higher amount of G-blocks	661:685	ALG1 having higher amount of G-blocks was favourable in the complex formation with ATC.
32698067	5	1	dep	ratio	758:762	arg1	ALG					740:742	ALG	740:742	ALG	740:742	At ALG to ATC weight ratio being 0.4 to 1 and total concentration of 0.66 g/L, about 1.54 g of ATC per gram of ALG1 and about 1.24 g of ATC per gram of ALG2 was incorporated into the complex.
32698067	3	2	theme	ATC	490:492	arg1	amount					480:485	The amount	476:485	The amount of ATC incorporated into the complex	476:522	The amount of ATC incorporated into the complex depended on the type of ALG used, the ALG to ATC weight ratio and the total concentration of the components in the solution.
32698067	3	2	theme	ATC	490:492	arg1	ATC					490:492	ATC	490:492	ATC	490:492	The amount of ATC incorporated into the complex depended on the type of ALG used, the ALG to ATC weight ratio and the total concentration of the components in the solution.
32698067	6	3	theme	ATC	988:990	arg1	rate					972:975	the rate	968:975	the rate of ALG and ATC complex decomposition	968:1012	Thermogravimetric analysis showed that the rate of ALG and ATC complex decomposition also depended on the type of ALG used and was 2.5 times higher for ALG2 and ATC complex compared to ALG1 and ATC complex.
32698067	6	3	theme	ATC	988:990	arg1	higher					1070:1075	higher	1070:1075	higher	1070:1075	Thermogravimetric analysis showed that the rate of ALG and ATC complex decomposition also depended on the type of ALG used and was 2.5 times higher for ALG2 and ATC complex compared to ALG1 and ATC complex.
32698067	3	4	theme	ALG	548:550	arg1	type					540:543	the type	536:543	the type of ALG used, the ALG to ATC weight ratio and the total concentration of the components in the solution	536:646	The amount of ATC incorporated into the complex depended on the type of ALG used, the ALG to ATC weight ratio and the total concentration of the components in the solution.
32698067	4	5	theme	complex	709:715	arg1	formation					717:725	the complex formation	705:725	the complex formation with ATC	705:734	ALG1 having higher amount of G-blocks was favourable in the complex formation with ATC.
32698067	4	6	from	formation	717:725	arg1	favourable					691:700	favourable	691:700	favourable	691:700	ALG1 having higher amount of G-blocks was favourable in the complex formation with ATC.
32698067	1	7	theme	Sodium	69:74	arg1	alginates					76:84	Sodium alginates	69:84	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2	69:151	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	3	8	from	concentration	600:612	arg1	solution					639:646	the solution	635:646	the solution	635:646	The amount of ATC incorporated into the complex depended on the type of ALG used, the ALG to ATC weight ratio and the total concentration of the components in the solution.
32698067	1	9	theme	flavylium	365:373	arg1	cations					375:381	flavylium cations	365:381	flavylium cations	365:381	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	6	10	theme	ALG	980:982	arg1	rate					972:975	the rate	968:975	the rate of ALG and ATC complex decomposition	968:1012	Thermogravimetric analysis showed that the rate of ALG and ATC complex decomposition also depended on the type of ALG used and was 2.5 times higher for ALG2 and ATC complex compared to ALG1 and ATC complex.
32698067	6	10	theme	ALG	980:982	arg1	higher					1070:1075	higher	1070:1075	higher	1070:1075	Thermogravimetric analysis showed that the rate of ALG and ATC complex decomposition also depended on the type of ALG used and was 2.5 times higher for ALG2 and ATC complex compared to ALG1 and ATC complex.
32698067	1	11	dep	units	127:131	arg1	ALG1					139:142	ratio ALG1	133:142	ratio ALG1	133:142	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	1	11	dep	units	127:131	arg1	units					127:131	guluronate units ratio ALG1 and ALG2	116:151	guluronate units ratio ALG1 and ALG2	116:151	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	1	11	dep	units	127:131	arg1	ALG2					148:151	ALG2	148:151	ALG2	148:151	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	5	12	theme	total	783:787	arg1	concentration					789:801	total concentration	783:801	total concentration of 0.66 g/L	783:813	At ALG to ATC weight ratio being 0.4 to 1 and total concentration of 0.66 g/L, about 1.54 g of ATC per gram of ALG1 and about 1.24 g of ATC per gram of ALG2 was incorporated into the complex.
32698067	0	13	theme	anthocyanin	46:56	arg1	complexes					58:66	anthocyanin complexes	46:66	anthocyanin complexes	46:66	Formation and characteristics of alginate and anthocyanin complexes.
32698067	6	14	theme	ATC	1123:1125	arg1	complex					1127:1133	ATC complex	1123:1133	ATC complex	1123:1133	Thermogravimetric analysis showed that the rate of ALG and ATC complex decomposition also depended on the type of ALG used and was 2.5 times higher for ALG2 and ATC complex compared to ALG1 and ATC complex.
32698067	5	15	theme	ALG1	848:851	arg1	gram					840:843	gram	840:843	gram of ALG1	840:851	At ALG to ATC weight ratio being 0.4 to 1 and total concentration of 0.66 g/L, about 1.54 g of ATC per gram of ALG1 and about 1.24 g of ATC per gram of ALG2 was incorporated into the complex.
32698067	3	16	from	ratio	580:584	arg1	solution					639:646	the solution	635:646	the solution	635:646	The amount of ATC incorporated into the complex depended on the type of ALG used, the ALG to ATC weight ratio and the total concentration of the components in the solution.
32698067	2	17	theme	Monomer	391:397	arg1	composition					399:409	Monomer composition	391:409	Monomer composition of ALG1 and ALG2	391:426	Monomer composition of ALG1 and ALG2 was determined from the 13C CP-MAS NMR spectra.
32698067	5	18	theme	ATC	747:749	arg1	ratio					758:762	ATC weight ratio	747:762	ATC weight ratio being 0.4 to 1	747:777	At ALG to ATC weight ratio being 0.4 to 1 and total concentration of 0.66 g/L, about 1.54 g of ATC per gram of ALG1 and about 1.24 g of ATC per gram of ALG2 was incorporated into the complex.
32698067	1	19	theme	different	91:99	arg1	mannuronate					101:111	different mannuronate	91:111	different mannuronate to guluronate units ratio ALG1 and ALG2	91:151	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	5	20	theme	weight	751:756	arg1	ratio					758:762	ATC weight ratio	747:762	ATC weight ratio being 0.4 to 1	747:777	At ALG to ATC weight ratio being 0.4 to 1 and total concentration of 0.66 g/L, about 1.54 g of ATC per gram of ALG1 and about 1.24 g of ATC per gram of ALG2 was incorporated into the complex.
32698067	4	21	from	favourable	691:700	arg1	formation					717:725	the complex formation	705:725	the complex formation with ATC	705:734	ALG1 having higher amount of G-blocks was favourable in the complex formation with ATC.
32698067	6	22	theme	ALG	1043:1045	arg1	type					1035:1038	the type	1031:1038	the type of ALG used	1031:1050	Thermogravimetric analysis showed that the rate of ALG and ATC complex decomposition also depended on the type of ALG used and was 2.5 times higher for ALG2 and ATC complex compared to ALG1 and ATC complex.
32698067	1	23	theme	insoluble	187:195	arg1	complexes					197:205	insoluble complexes	187:205	insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC	187:388	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	1	24	theme	electrostatic	283:295	arg1	interaction					297:307	the electrostatic interaction	279:307	the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC	279:388	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	6	25	dep	ALG	980:982	arg1	decomposition					1000:1012	complex decomposition	992:1012	complex decomposition	992:1012	Thermogravimetric analysis showed that the rate of ALG and ATC complex decomposition also depended on the type of ALG used and was 2.5 times higher for ALG2 and ATC complex compared to ALG1 and ATC complex.
32698067	1	26	theme	complexes	197:205	arg1	formation					174:182	the formation	170:182	the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC	170:388	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	4	27	with	formation	717:725	arg1	ATC					732:734	ATC	732:734	ATC	732:734	ALG1 having higher amount of G-blocks was favourable in the complex formation with ATC.
32698067	1	28	theme	ATC	386:388	arg1	ALG					356:358	ALG	356:358	ALG	356:358	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	1	28	theme	ATC	386:388	arg1	groups					329:334	carboxylate groups	317:334	carboxylate groups of sodium alginate (ALG)	317:359	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	1	28	theme	ATC	386:388	arg1	ATC					386:388	ATC	386:388	ATC	386:388	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	1	28	theme	ATC	386:388	arg1	cations					375:381	flavylium cations	365:381	flavylium cations	365:381	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	1	28	theme	ATC	386:388	arg1	alginate					346:353	sodium alginate	339:353	sodium alginate (ALG)	339:359	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	1	29	theme	guluronate	116:125	arg1	ALG1					139:142	ratio ALG1	133:142	ratio ALG1	133:142	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	1	29	theme	guluronate	116:125	arg1	units					127:131	guluronate units ratio ALG1 and ALG2	116:151	guluronate units ratio ALG1 and ALG2	116:151	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	1	29	theme	guluronate	116:125	arg1	ALG2					148:151	ALG2	148:151	ALG2	148:151	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	7	30	theme	antioxidant	1184:1194	arg1	activity					1196:1203	prolonged antioxidant activity	1174:1203	prolonged antioxidant activity	1174:1203	Furthermore, ALG/ATC complexes showed prolonged antioxidant activity when compared to ATC.
32698067	1	31	with	alginates	76:84	arg1	mannuronate					101:111	different mannuronate	91:111	different mannuronate to guluronate units ratio ALG1 and ALG2	91:151	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	4	32	theme	higher	661:666	arg1	amount					668:673	higher amount	661:673	higher amount of G-blocks	661:685	ALG1 having higher amount of G-blocks was favourable in the complex formation with ATC.
32698067	4	32	theme	higher	661:666	arg1	G-blocks					678:685	G-blocks	678:685	G-blocks	678:685	ALG1 having higher amount of G-blocks was favourable in the complex formation with ATC.
32698067	1	33	with	complexes	197:205	arg1	ATC					226:228	ATC	226:228	ATC	226:228	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	1	33	with	complexes	197:205	arg1	anthocyanins					212:223	anthocyanins	212:223	anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC	212:388	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	5	34	theme	0.66 g/L	806:813	arg1	concentration					789:801	total concentration	783:801	total concentration of 0.66 g/L	783:813	At ALG to ATC weight ratio being 0.4 to 1 and total concentration of 0.66 g/L, about 1.54 g of ATC per gram of ALG1 and about 1.24 g of ATC per gram of ALG2 was incorporated into the complex.
32698067	5	34	theme	0.66 g/L	806:813	arg1	ratio					758:762	ATC weight ratio	747:762	ATC weight ratio being 0.4 to 1	747:777	At ALG to ATC weight ratio being 0.4 to 1 and total concentration of 0.66 g/L, about 1.54 g of ATC per gram of ALG1 and about 1.24 g of ATC per gram of ALG2 was incorporated into the complex.
32698067	2	35	theme	ALG1	414:417	arg1	composition					399:409	Monomer composition	391:409	Monomer composition of ALG1 and ALG2	391:426	Monomer composition of ALG1 and ALG2 was determined from the 13C CP-MAS NMR spectra.
32698067	0	36	theme	alginate	33:40	arg1	characteristics					14:28	characteristics	14:28	characteristics	14:28	Formation and characteristics of alginate and anthocyanin complexes.
32698067	0	36	theme	alginate	33:40	arg1	Formation					0:8	Formation	0:8	Formation	0:8	Formation and characteristics of alginate and anthocyanin complexes.
32698067	1	37	theme	carboxylate	317:327	arg1	ALG					356:358	ALG	356:358	ALG	356:358	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	1	37	theme	carboxylate	317:327	arg1	groups					329:334	carboxylate groups	317:334	carboxylate groups of sodium alginate (ALG)	317:359	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	1	37	theme	carboxylate	317:327	arg1	ATC					386:388	ATC	386:388	ATC	386:388	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	1	37	theme	carboxylate	317:327	arg1	alginate					346:353	sodium alginate	339:353	sodium alginate (ALG)	339:359	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	2	38	theme	NMR	463:465	arg1	spectra					467:473	the 13C CP-MAS NMR spectra	448:473	the 13C CP-MAS NMR spectra	448:473	Monomer composition of ALG1 and ALG2 was determined from the 13C CP-MAS NMR spectra.
32698067	6	39	theme	Thermogravimetric	929:945	arg1	analysis					947:954	Thermogravimetric analysis	929:954	Thermogravimetric analysis	929:954	Thermogravimetric analysis showed that the rate of ALG and ATC complex decomposition also depended on the type of ALG used and was 2.5 times higher for ALG2 and ATC complex compared to ALG1 and ATC complex.
32698067	0	40	theme	complexes	58:66	arg1	characteristics					14:28	characteristics	14:28	characteristics	14:28	Formation and characteristics of alginate and anthocyanin complexes.
32698067	0	40	theme	complexes	58:66	arg1	Formation					0:8	Formation	0:8	Formation	0:8	Formation and characteristics of alginate and anthocyanin complexes.
32698067	3	41	theme	total	594:598	arg1	concentration					600:612	the total concentration	590:612	the total concentration of the components in the solution	590:646	The amount of ATC incorporated into the complex depended on the type of ALG used, the ALG to ATC weight ratio and the total concentration of the components in the solution.
32698067	5	42	theme	ATC	873:875	arg1	1.24 g					863:868	1.24 g	863:868	1.24 g of ATC per gram of ALG2	863:892	At ALG to ATC weight ratio being 0.4 to 1 and total concentration of 0.66 g/L, about 1.54 g of ATC per gram of ALG1 and about 1.24 g of ATC per gram of ALG2 was incorporated into the complex.
32698067	5	43	theme	ATC	832:834	arg1	1.54 g					822:827	1.54 g	822:827	1.54 g	822:827	At ALG to ATC weight ratio being 0.4 to 1 and total concentration of 0.66 g/L, about 1.54 g of ATC per gram of ALG1 and about 1.24 g of ATC per gram of ALG2 was incorporated into the complex.
32698067	5	43	theme	ATC	832:834	arg1	ATC					832:834	ATC	832:834	ATC per gram of ALG1 and about 1.24 g of ATC per gram of ALG2	832:892	At ALG to ATC weight ratio being 0.4 to 1 and total concentration of 0.66 g/L, about 1.54 g of ATC per gram of ALG1 and about 1.24 g of ATC per gram of ALG2 was incorporated into the complex.
32698067	2	44	theme	13C	452:454	arg1	spectra					467:473	the 13C CP-MAS NMR spectra	448:473	the 13C CP-MAS NMR spectra	448:473	Monomer composition of ALG1 and ALG2 was determined from the 13C CP-MAS NMR spectra.
32698067	6	45	theme	ATC	1090:1092	arg1	complex					1094:1100	ATC complex	1090:1100	ATC complex	1090:1100	Thermogravimetric analysis showed that the rate of ALG and ATC complex decomposition also depended on the type of ALG used and was 2.5 times higher for ALG2 and ATC complex compared to ALG1 and ATC complex.
32698067	1	46	theme	ratio	133:137	arg1	ALG1					139:142	ratio ALG1	133:142	ratio ALG1	133:142	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	1	46	theme	ratio	133:137	arg1	units					127:131	guluronate units ratio ALG1 and ALG2	116:151	guluronate units ratio ALG1 and ALG2	116:151	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	1	47	theme	sodium	339:344	arg1	ALG					356:358	ALG	356:358	ALG	356:358	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	1	47	theme	sodium	339:344	arg1	alginate					346:353	sodium alginate	339:353	sodium alginate (ALG)	339:359	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	5	48	dep	1	777:777	arg1	to					774:775	to	774:775	to	774:775	At ALG to ATC weight ratio being 0.4 to 1 and total concentration of 0.66 g/L, about 1.54 g of ATC per gram of ALG1 and about 1.24 g of ATC per gram of ALG2 was incorporated into the complex.
32698067	4	49	theme	G-blocks	678:685	arg1	amount					668:673	higher amount	661:673	higher amount of G-blocks	661:685	ALG1 having higher amount of G-blocks was favourable in the complex formation with ATC.
32698067	4	49	theme	G-blocks	678:685	arg1	G-blocks					678:685	G-blocks	678:685	G-blocks	678:685	ALG1 having higher amount of G-blocks was favourable in the complex formation with ATC.
32698067	7	50	theme	ALG/ATC	1149:1155	arg1	complexes					1157:1165	ALG/ATC complexes	1149:1165	ALG/ATC complexes	1149:1165	Furthermore, ALG/ATC complexes showed prolonged antioxidant activity when compared to ATC.
32698067	5	51	theme	ALG2	889:892	arg1	gram					881:884	gram	881:884	gram of ALG2	881:892	At ALG to ATC weight ratio being 0.4 to 1 and total concentration of 0.66 g/L, about 1.54 g of ATC per gram of ALG1 and about 1.24 g of ATC per gram of ALG2 was incorporated into the complex.
32698067	3	52	theme	components	621:630	arg1	concentration					600:612	the total concentration	590:612	the total concentration of the components in the solution	590:646	The amount of ATC incorporated into the complex depended on the type of ALG used, the ALG to ATC weight ratio and the total concentration of the components in the solution.
32698067	3	52	theme	components	621:630	arg1	ratio					580:584	ATC weight ratio	569:584	ATC weight ratio	569:584	The amount of ATC incorporated into the complex depended on the type of ALG used, the ALG to ATC weight ratio and the total concentration of the components in the solution.
32698067	3	53	theme	ATC	569:571	arg1	ratio					580:584	ATC weight ratio	569:584	ATC weight ratio	569:584	The amount of ATC incorporated into the complex depended on the type of ALG used, the ALG to ATC weight ratio and the total concentration of the components in the solution.
32698067	2	54	theme	ALG2	423:426	arg1	composition					399:409	Monomer composition	391:409	Monomer composition of ALG1 and ALG2	391:426	Monomer composition of ALG1 and ALG2 was determined from the 13C CP-MAS NMR spectra.
32698067	7	55	theme	prolonged	1174:1182	arg1	activity					1196:1203	prolonged antioxidant activity	1174:1203	prolonged antioxidant activity	1174:1203	Furthermore, ALG/ATC complexes showed prolonged antioxidant activity when compared to ATC.
32698067	2	56	theme	CP-MAS	456:461	arg1	spectra					467:473	the 13C CP-MAS NMR spectra	448:473	the 13C CP-MAS NMR spectra	448:473	Monomer composition of ALG1 and ALG2 was determined from the 13C CP-MAS NMR spectra.
32698067	1	57	theme	alginate	346:353	arg1	ALG					356:358	ALG	356:358	ALG	356:358	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	1	57	theme	alginate	346:353	arg1	groups					329:334	carboxylate groups	317:334	carboxylate groups of sodium alginate (ALG)	317:359	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	1	57	theme	alginate	346:353	arg1	ATC					386:388	ATC	386:388	ATC	386:388	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	1	57	theme	alginate	346:353	arg1	cations					375:381	flavylium cations	365:381	flavylium cations	365:381	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	1	57	theme	alginate	346:353	arg1	alginate					346:353	sodium alginate	339:353	sodium alginate (ALG)	339:359	Sodium alginates with different mannuronate to guluronate units ratio ALG1 and ALG2 were employed in the formation of insoluble complexes with anthocyanins (ATC) extracted from Vaccinium myrtilus by exploiting the electrostatic interaction between carboxylate groups of sodium alginate (ALG) and flavylium cations of ATC.
32698067	3	58	theme	weight	573:578	arg1	ratio					580:584	ATC weight ratio	569:584	ATC weight ratio	569:584	The amount of ATC incorporated into the complex depended on the type of ALG used, the ALG to ATC weight ratio and the total concentration of the components in the solution.
32698067	6	59	theme	complex	992:998	arg1	decomposition					1000:1012	complex decomposition	992:1012	complex decomposition	992:1012	Thermogravimetric analysis showed that the rate of ALG and ATC complex decomposition also depended on the type of ALG used and was 2.5 times higher for ALG2 and ATC complex compared to ALG1 and ATC complex.
33646339	4	0	theme	microscopy	623:632	arg1	analysis					634:641	the transmission electron microscopy analysis	597:641	the transmission electron microscopy analysis	597:641	SDS-PAGE and Calcofluor White Stain (CWS) staining indicated that the obtained CSCs were protein-free and the transmission electron microscopy analysis showed that CSCs performed the intact and loose chitin spore coats.
33646339	1	1	theme	economic	214:221	arg1	loss					223:226	great economic loss	208:226	great economic loss	208:226	Nosema ceranae is the pathogen of nosemosis in the honey bee, which can bring great economic loss to apiculture.
33646339	5	2	theme	Western	711:717	arg1	blotting					719:726	Western blotting	711:726	Western blotting	711:726	Western blotting and indirect immunofluorescence analysis (IFA) demonstrated that CSCs could interact with three spore wall proteins (rNcSWP7, rNcSWP8, and rNcSWP12).
33646339	5	3	theme	indirect	732:739	arg1	immunofluorescence					741:758	indirect immunofluorescence	732:758	indirect immunofluorescence	732:758	Western blotting and indirect immunofluorescence analysis (IFA) demonstrated that CSCs could interact with three spore wall proteins (rNcSWP7, rNcSWP8, and rNcSWP12).
33646339	4	4	theme	White	515:519	arg1	staining					533:540	Calcofluor White Stain (CWS) staining	504:540	Calcofluor White Stain (CWS) staining	504:540	SDS-PAGE and Calcofluor White Stain (CWS) staining indicated that the obtained CSCs were protein-free and the transmission electron microscopy analysis showed that CSCs performed the intact and loose chitin spore coats.
33646339	3	5	theme	alkaline	471:478	arg1	treatment					480:488	optimized hot alkaline treatment	457:488	optimized hot alkaline treatment	457:488	Here, Chitin Spore Coats (CSCs) of N. ceranae were successfully extracted by optimized hot alkaline treatment.
33646339	5	6	dep	proteins	835:842	arg1	rNcSWP8					854:860	rNcSWP8	854:860	rNcSWP8	854:860	Western blotting and indirect immunofluorescence analysis (IFA) demonstrated that CSCs could interact with three spore wall proteins (rNcSWP7, rNcSWP8, and rNcSWP12).
33646339	5	6	dep	proteins	835:842	arg1	rNcSWP12					867:874	rNcSWP12	867:874	rNcSWP12	867:874	Western blotting and indirect immunofluorescence analysis (IFA) demonstrated that CSCs could interact with three spore wall proteins (rNcSWP7, rNcSWP8, and rNcSWP12).
33646339	5	6	dep	proteins	835:842	arg1	rNcSWP7					845:851	rNcSWP7	845:851	rNcSWP7	845:851	Western blotting and indirect immunofluorescence analysis (IFA) demonstrated that CSCs could interact with three spore wall proteins (rNcSWP7, rNcSWP8, and rNcSWP12).
33646339	5	6	dep	proteins	835:842	arg1	proteins					835:842	three spore wall proteins	818:842	three spore wall proteins (rNcSWP7, rNcSWP8, and rNcSWP12)	818:875	Western blotting and indirect immunofluorescence analysis (IFA) demonstrated that CSCs could interact with three spore wall proteins (rNcSWP7, rNcSWP8, and rNcSWP12).
33646339	4	7	theme	CWS	528:530	arg1	staining					533:540	Calcofluor White Stain (CWS) staining	504:540	Calcofluor White Stain (CWS) staining	504:540	SDS-PAGE and Calcofluor White Stain (CWS) staining indicated that the obtained CSCs were protein-free and the transmission electron microscopy analysis showed that CSCs performed the intact and loose chitin spore coats.
33646339	4	8	theme	spore	698:702	arg1	coats					704:708	the intact and loose chitin spore coats	670:708	the intact and loose chitin spore coats	670:708	SDS-PAGE and Calcofluor White Stain (CWS) staining indicated that the obtained CSCs were protein-free and the transmission electron microscopy analysis showed that CSCs performed the intact and loose chitin spore coats.
33646339	6	9	theme	extract	906:912	arg1	CSCs					914:917	extract CSCs	906:917	extract CSCs of N. ceranae	906:931	Our method was effective to extract CSCs of N. ceranae and this could be very useful for screening spore wall proteins involved in endospore composition, which could be helpful to uncover the biological structure and pathogenesis of microsporidia.
33646339	0	10	theme	interactive	97:107	arg1	proteins					120:127	the interactive spore wall proteins	93:127	the interactive spore wall proteins	93:127	Isolation of protein-free chitin spore coats of Nosema ceranae and its application to screen the interactive spore wall proteins.
33646339	3	11	theme	N.	415:416	arg1	ceranae					418:424	N. ceranae	415:424	N. ceranae	415:424	Here, Chitin Spore Coats (CSCs) of N. ceranae were successfully extracted by optimized hot alkaline treatment.
33646339	3	12	theme	optimized	457:465	arg1	treatment					480:488	optimized hot alkaline treatment	457:488	optimized hot alkaline treatment	457:488	Here, Chitin Spore Coats (CSCs) of N. ceranae were successfully extracted by optimized hot alkaline treatment.
33646339	4	13	theme	transmission	601:612	arg1	microscopy					623:632	the transmission electron microscopy	597:632	the transmission electron microscopy analysis	597:641	SDS-PAGE and Calcofluor White Stain (CWS) staining indicated that the obtained CSCs were protein-free and the transmission electron microscopy analysis showed that CSCs performed the intact and loose chitin spore coats.
33646339	4	14	theme	Stain	521:525	arg1	staining					533:540	Calcofluor White Stain (CWS) staining	504:540	Calcofluor White Stain (CWS) staining	504:540	SDS-PAGE and Calcofluor White Stain (CWS) staining indicated that the obtained CSCs were protein-free and the transmission electron microscopy analysis showed that CSCs performed the intact and loose chitin spore coats.
33646339	3	15	theme	hot	467:469	arg1	treatment					480:488	optimized hot alkaline treatment	457:488	optimized hot alkaline treatment	457:488	Here, Chitin Spore Coats (CSCs) of N. ceranae were successfully extracted by optimized hot alkaline treatment.
33646339	3	16	theme	Chitin	386:391	arg1	Coats					399:403	Chitin Spore Coats	386:403	Chitin Spore Coats (CSCs) of N. ceranae	386:424	Here, Chitin Spore Coats (CSCs) of N. ceranae were successfully extracted by optimized hot alkaline treatment.
33646339	3	16	theme	Chitin	386:391	arg1	CSCs					406:409	CSCs	406:409	CSCs	406:409	Here, Chitin Spore Coats (CSCs) of N. ceranae were successfully extracted by optimized hot alkaline treatment.
33646339	5	17	dep	blotting	719:726	arg1	IFA					770:772	IFA	770:772	IFA	770:772	Western blotting and indirect immunofluorescence analysis (IFA) demonstrated that CSCs could interact with three spore wall proteins (rNcSWP7, rNcSWP8, and rNcSWP12).
33646339	5	17	dep	blotting	719:726	arg1	analysis					760:767	analysis	760:767	analysis (IFA)	760:773	Western blotting and indirect immunofluorescence analysis (IFA) demonstrated that CSCs could interact with three spore wall proteins (rNcSWP7, rNcSWP8, and rNcSWP12).
33646339	1	18	theme	nosemosis	164:172	arg1	ceranae					137:143	Nosema ceranae	130:143	Nosema ceranae	130:143	Nosema ceranae is the pathogen of nosemosis in the honey bee, which can bring great economic loss to apiculture.
33646339	1	18	theme	nosemosis	164:172	arg1	pathogen					152:159	the pathogen	148:159	the pathogen of nosemosis in the honey bee, which can bring great economic loss to apiculture	148:240	Nosema ceranae is the pathogen of nosemosis in the honey bee, which can bring great economic loss to apiculture.
33646339	4	19	theme	intact	674:679	arg1	coats					704:708	the intact and loose chitin spore coats	670:708	the intact and loose chitin spore coats	670:708	SDS-PAGE and Calcofluor White Stain (CWS) staining indicated that the obtained CSCs were protein-free and the transmission electron microscopy analysis showed that CSCs performed the intact and loose chitin spore coats.
33646339	6	20	theme	N.	922:923	arg1	ceranae					925:931	N. ceranae	922:931	N. ceranae	922:931	Our method was effective to extract CSCs of N. ceranae and this could be very useful for screening spore wall proteins involved in endospore composition, which could be helpful to uncover the biological structure and pathogenesis of microsporidia.
33646339	5	21	theme	spore	824:828	arg1	rNcSWP8					854:860	rNcSWP8	854:860	rNcSWP8	854:860	Western blotting and indirect immunofluorescence analysis (IFA) demonstrated that CSCs could interact with three spore wall proteins (rNcSWP7, rNcSWP8, and rNcSWP12).
33646339	5	21	theme	spore	824:828	arg1	rNcSWP12					867:874	rNcSWP12	867:874	rNcSWP12	867:874	Western blotting and indirect immunofluorescence analysis (IFA) demonstrated that CSCs could interact with three spore wall proteins (rNcSWP7, rNcSWP8, and rNcSWP12).
33646339	5	21	theme	spore	824:828	arg1	rNcSWP7					845:851	rNcSWP7	845:851	rNcSWP7	845:851	Western blotting and indirect immunofluorescence analysis (IFA) demonstrated that CSCs could interact with three spore wall proteins (rNcSWP7, rNcSWP8, and rNcSWP12).
33646339	5	21	theme	spore	824:828	arg1	proteins					835:842	three spore wall proteins	818:842	three spore wall proteins (rNcSWP7, rNcSWP8, and rNcSWP12)	818:875	Western blotting and indirect immunofluorescence analysis (IFA) demonstrated that CSCs could interact with three spore wall proteins (rNcSWP7, rNcSWP8, and rNcSWP12).
33646339	0	22	theme	chitin	26:31	arg1	coats					39:43	protein-free chitin spore coats	13:43	protein-free chitin spore coats of Nosema ceranae	13:61	Isolation of protein-free chitin spore coats of Nosema ceranae and its application to screen the interactive spore wall proteins.
33646339	0	23	theme	wall	115:118	arg1	proteins					120:127	the interactive spore wall proteins	93:127	the interactive spore wall proteins	93:127	Isolation of protein-free chitin spore coats of Nosema ceranae and its application to screen the interactive spore wall proteins.
33646339	2	24	theme	major	260:264	arg1	role					333:336	an essential role	320:336	an essential role	320:336	Chitin acts as a major component of the endospore of microsporidia and plays an essential role to form the bridges across the endospore.
33646339	2	24	theme	major	260:264	arg1	component					266:274	a major component	258:274	a major component of the endospore of microsporidia	258:308	Chitin acts as a major component of the endospore of microsporidia and plays an essential role to form the bridges across the endospore.
33646339	2	24	theme	major	260:264	arg1	Chitin					243:248	Chitin	243:248	Chitin	243:248	Chitin acts as a major component of the endospore of microsporidia and plays an essential role to form the bridges across the endospore.
33646339	4	25	theme	obtained	561:568	arg1	CSCs					570:573	the obtained CSCs	557:573	the obtained CSCs	557:573	SDS-PAGE and Calcofluor White Stain (CWS) staining indicated that the obtained CSCs were protein-free and the transmission electron microscopy analysis showed that CSCs performed the intact and loose chitin spore coats.
33646339	4	25	theme	obtained	561:568	arg1	protein-free					580:591	protein-free	580:591	protein-free	580:591	SDS-PAGE and Calcofluor White Stain (CWS) staining indicated that the obtained CSCs were protein-free and the transmission electron microscopy analysis showed that CSCs performed the intact and loose chitin spore coats.
33646339	0	26	theme	protein-free	13:24	arg1	coats					39:43	protein-free chitin spore coats	13:43	protein-free chitin spore coats of Nosema ceranae	13:61	Isolation of protein-free chitin spore coats of Nosema ceranae and its application to screen the interactive spore wall proteins.
33646339	0	27	theme	spore	109:113	arg1	proteins					120:127	the interactive spore wall proteins	93:127	the interactive spore wall proteins	93:127	Isolation of protein-free chitin spore coats of Nosema ceranae and its application to screen the interactive spore wall proteins.
33646339	4	28	theme	loose	685:689	arg1	coats					704:708	the intact and loose chitin spore coats	670:708	the intact and loose chitin spore coats	670:708	SDS-PAGE and Calcofluor White Stain (CWS) staining indicated that the obtained CSCs were protein-free and the transmission electron microscopy analysis showed that CSCs performed the intact and loose chitin spore coats.
33646339	1	29	theme	honey	181:185	arg1	bee					187:189	the honey bee	177:189	the honey bee	177:189	Nosema ceranae is the pathogen of nosemosis in the honey bee, which can bring great economic loss to apiculture.
33646339	0	30	theme	coats	39:43	arg1	Isolation					0:8	Isolation	0:8	Isolation of protein-free chitin spore coats of Nosema ceranae	0:61	Isolation of protein-free chitin spore coats of Nosema ceranae and its application to screen the interactive spore wall proteins.
33646339	0	30	theme	coats	39:43	arg1	application					71:81	its application to screen the interactive spore wall proteins	67:127	its application to screen the interactive spore wall proteins	67:127	Isolation of protein-free chitin spore coats of Nosema ceranae and its application to screen the interactive spore wall proteins.
33646339	3	31	theme	Spore	393:397	arg1	Coats					399:403	Chitin Spore Coats	386:403	Chitin Spore Coats (CSCs) of N. ceranae	386:424	Here, Chitin Spore Coats (CSCs) of N. ceranae were successfully extracted by optimized hot alkaline treatment.
33646339	3	31	theme	Spore	393:397	arg1	CSCs					406:409	CSCs	406:409	CSCs	406:409	Here, Chitin Spore Coats (CSCs) of N. ceranae were successfully extracted by optimized hot alkaline treatment.
33646339	0	32	theme	spore	33:37	arg1	coats					39:43	protein-free chitin spore coats	13:43	protein-free chitin spore coats of Nosema ceranae	13:61	Isolation of protein-free chitin spore coats of Nosema ceranae and its application to screen the interactive spore wall proteins.
33646339	6	33	theme	spore	977:981	arg1	proteins					988:995	spore wall proteins	977:995	spore wall proteins	977:995	Our method was effective to extract CSCs of N. ceranae and this could be very useful for screening spore wall proteins involved in endospore composition, which could be helpful to uncover the biological structure and pathogenesis of microsporidia.
33646339	0	34	theme	Nosema	48:53	arg1	ceranae					55:61	Nosema ceranae	48:61	Nosema ceranae	48:61	Isolation of protein-free chitin spore coats of Nosema ceranae and its application to screen the interactive spore wall proteins.
33646339	5	35	theme	wall	830:833	arg1	rNcSWP8					854:860	rNcSWP8	854:860	rNcSWP8	854:860	Western blotting and indirect immunofluorescence analysis (IFA) demonstrated that CSCs could interact with three spore wall proteins (rNcSWP7, rNcSWP8, and rNcSWP12).
33646339	5	35	theme	wall	830:833	arg1	rNcSWP12					867:874	rNcSWP12	867:874	rNcSWP12	867:874	Western blotting and indirect immunofluorescence analysis (IFA) demonstrated that CSCs could interact with three spore wall proteins (rNcSWP7, rNcSWP8, and rNcSWP12).
33646339	5	35	theme	wall	830:833	arg1	rNcSWP7					845:851	rNcSWP7	845:851	rNcSWP7	845:851	Western blotting and indirect immunofluorescence analysis (IFA) demonstrated that CSCs could interact with three spore wall proteins (rNcSWP7, rNcSWP8, and rNcSWP12).
33646339	5	35	theme	wall	830:833	arg1	proteins					835:842	three spore wall proteins	818:842	three spore wall proteins (rNcSWP7, rNcSWP8, and rNcSWP12)	818:875	Western blotting and indirect immunofluorescence analysis (IFA) demonstrated that CSCs could interact with three spore wall proteins (rNcSWP7, rNcSWP8, and rNcSWP12).
33646339	2	36	theme	microsporidia	296:308	arg1	endospore					283:291	the endospore	279:291	the endospore of microsporidia	279:308	Chitin acts as a major component of the endospore of microsporidia and plays an essential role to form the bridges across the endospore.
33646339	3	37	theme	ceranae	418:424	arg1	Coats					399:403	Chitin Spore Coats	386:403	Chitin Spore Coats (CSCs) of N. ceranae	386:424	Here, Chitin Spore Coats (CSCs) of N. ceranae were successfully extracted by optimized hot alkaline treatment.
33646339	3	37	theme	ceranae	418:424	arg1	CSCs					406:409	CSCs	406:409	CSCs	406:409	Here, Chitin Spore Coats (CSCs) of N. ceranae were successfully extracted by optimized hot alkaline treatment.
33646339	1	38	theme	Nosema	130:135	arg1	ceranae					137:143	Nosema ceranae	130:143	Nosema ceranae	130:143	Nosema ceranae is the pathogen of nosemosis in the honey bee, which can bring great economic loss to apiculture.
33646339	1	38	theme	Nosema	130:135	arg1	pathogen					152:159	the pathogen	148:159	the pathogen of nosemosis in the honey bee, which can bring great economic loss to apiculture	148:240	Nosema ceranae is the pathogen of nosemosis in the honey bee, which can bring great economic loss to apiculture.
33646339	2	39	theme	endospore	283:291	arg1	role					333:336	an essential role	320:336	an essential role	320:336	Chitin acts as a major component of the endospore of microsporidia and plays an essential role to form the bridges across the endospore.
33646339	2	39	theme	endospore	283:291	arg1	component					266:274	a major component	258:274	a major component of the endospore of microsporidia	258:308	Chitin acts as a major component of the endospore of microsporidia and plays an essential role to form the bridges across the endospore.
33646339	2	39	theme	endospore	283:291	arg1	Chitin					243:248	Chitin	243:248	Chitin	243:248	Chitin acts as a major component of the endospore of microsporidia and plays an essential role to form the bridges across the endospore.
33646339	4	40	theme	chitin	691:696	arg1	coats					704:708	the intact and loose chitin spore coats	670:708	the intact and loose chitin spore coats	670:708	SDS-PAGE and Calcofluor White Stain (CWS) staining indicated that the obtained CSCs were protein-free and the transmission electron microscopy analysis showed that CSCs performed the intact and loose chitin spore coats.
33646339	6	41	theme	biological	1070:1079	arg1	structure					1081:1089	biological structure	1070:1089	biological structure	1070:1089	Our method was effective to extract CSCs of N. ceranae and this could be very useful for screening spore wall proteins involved in endospore composition, which could be helpful to uncover the biological structure and pathogenesis of microsporidia.
33646339	6	42	theme	endospore	1009:1017	arg1	composition					1019:1029	endospore composition	1009:1029	endospore composition	1009:1029	Our method was effective to extract CSCs of N. ceranae and this could be very useful for screening spore wall proteins involved in endospore composition, which could be helpful to uncover the biological structure and pathogenesis of microsporidia.
33646339	6	43	theme	microsporidia	1111:1123	arg1	structure					1081:1089	biological structure	1070:1089	biological structure	1070:1089	Our method was effective to extract CSCs of N. ceranae and this could be very useful for screening spore wall proteins involved in endospore composition, which could be helpful to uncover the biological structure and pathogenesis of microsporidia.
33646339	6	43	theme	microsporidia	1111:1123	arg1	pathogenesis					1095:1106	pathogenesis	1095:1106	pathogenesis	1095:1106	Our method was effective to extract CSCs of N. ceranae and this could be very useful for screening spore wall proteins involved in endospore composition, which could be helpful to uncover the biological structure and pathogenesis of microsporidia.
33646339	6	44	dep	structure	1081:1089	arg1	the					1066:1068	the	1066:1068	the	1066:1068	Our method was effective to extract CSCs of N. ceranae and this could be very useful for screening spore wall proteins involved in endospore composition, which could be helpful to uncover the biological structure and pathogenesis of microsporidia.
33646339	2	45	theme	essential	323:331	arg1	role					333:336	an essential role	320:336	an essential role	320:336	Chitin acts as a major component of the endospore of microsporidia and plays an essential role to form the bridges across the endospore.
33646339	2	45	theme	essential	323:331	arg1	component					266:274	a major component	258:274	a major component of the endospore of microsporidia	258:308	Chitin acts as a major component of the endospore of microsporidia and plays an essential role to form the bridges across the endospore.
33646339	2	45	theme	essential	323:331	arg1	Chitin					243:248	Chitin	243:248	Chitin	243:248	Chitin acts as a major component of the endospore of microsporidia and plays an essential role to form the bridges across the endospore.
33646339	6	46	theme	wall	983:986	arg1	proteins					988:995	spore wall proteins	977:995	spore wall proteins	977:995	Our method was effective to extract CSCs of N. ceranae and this could be very useful for screening spore wall proteins involved in endospore composition, which could be helpful to uncover the biological structure and pathogenesis of microsporidia.
33646339	0	47	theme	ceranae	55:61	arg1	coats					39:43	protein-free chitin spore coats	13:43	protein-free chitin spore coats of Nosema ceranae	13:61	Isolation of protein-free chitin spore coats of Nosema ceranae and its application to screen the interactive spore wall proteins.
33646339	6	48	theme	ceranae	925:931	arg1	CSCs					914:917	extract CSCs	906:917	extract CSCs of N. ceranae	906:931	Our method was effective to extract CSCs of N. ceranae and this could be very useful for screening spore wall proteins involved in endospore composition, which could be helpful to uncover the biological structure and pathogenesis of microsporidia.
33646339	1	49	from	pathogen	152:159	arg1	bee					187:189	the honey bee	177:189	the honey bee	177:189	Nosema ceranae is the pathogen of nosemosis in the honey bee, which can bring great economic loss to apiculture.
33646339	4	50	theme	electron	614:621	arg1	microscopy					623:632	the transmission electron microscopy	597:632	the transmission electron microscopy analysis	597:641	SDS-PAGE and Calcofluor White Stain (CWS) staining indicated that the obtained CSCs were protein-free and the transmission electron microscopy analysis showed that CSCs performed the intact and loose chitin spore coats.
33646339	1	51	theme	great	208:212	arg1	loss					223:226	great economic loss	208:226	great economic loss	208:226	Nosema ceranae is the pathogen of nosemosis in the honey bee, which can bring great economic loss to apiculture.
32827623	9	0	from	process	1515:1521	arg1	adsorbent					1471:1479	adsorbent	1471:1479	adsorbent	1471:1479	The study emphasizes that the fabricated CAG spherical beads could act as a potential adsorbent in the water/wastewater treatment process.
32827623	9	0	from	process	1515:1521	arg1	beads					1440:1444	the fabricated CAG spherical beads	1411:1444	the fabricated CAG spherical beads	1411:1444	The study emphasizes that the fabricated CAG spherical beads could act as a potential adsorbent in the water/wastewater treatment process.
32827623	1	1	from	water	247:251	arg1	Acid					186:189	Acid Red 1	186:195	Acid Red 1 (AR1)	186:201	In order to remove noxious Congo Red (CR), Acid Red 1 (AR1) and Reactive Red 2 (RR2) dye molecules from water, an environment responsive and biodegradable spherical chitosan-gelatin biopolymeric beads were designed and embedded with Al3+ ions.
32827623	1	1	from	water	247:251	arg1	AR1					198:200	AR1	198:200	AR1	198:200	In order to remove noxious Congo Red (CR), Acid Red 1 (AR1) and Reactive Red 2 (RR2) dye molecules from water, an environment responsive and biodegradable spherical chitosan-gelatin biopolymeric beads were designed and embedded with Al3+ ions.
32827623	7	2	theme	molecular	1063:1071	arg1	force					1147:1151	the electrostatic force	1129:1151	the electrostatic force of attraction, surface complexation and hydrogen bondings that exist between dye molecules and the CAG spherical beads	1129:1270	The molecular interactions followed in the adsorption mechanisms were the electrostatic force of attraction, surface complexation and hydrogen bondings that exist between dye molecules and the CAG spherical beads.
32827623	7	2	theme	molecular	1063:1071	arg1	interactions					1073:1084	The molecular interactions	1059:1084	The molecular interactions followed in the adsorption mechanisms	1059:1122	The molecular interactions followed in the adsorption mechanisms were the electrostatic force of attraction, surface complexation and hydrogen bondings that exist between dye molecules and the CAG spherical beads.
32827623	9	3	theme	fabricated	1415:1424	arg1	adsorbent					1471:1479	adsorbent	1471:1479	adsorbent	1471:1479	The study emphasizes that the fabricated CAG spherical beads could act as a potential adsorbent in the water/wastewater treatment process.
32827623	9	3	theme	fabricated	1415:1424	arg1	beads					1440:1444	the fabricated CAG spherical beads	1411:1444	the fabricated CAG spherical beads	1411:1444	The study emphasizes that the fabricated CAG spherical beads could act as a potential adsorbent in the water/wastewater treatment process.
32827623	5	4	theme	CR	860:861	arg1	efficiency					846:855	The maximum adsorption efficiency	823:855	The maximum adsorption efficiency of CR, AR1, and RR2 dye molecules on CAG spherical beads	823:912	The maximum adsorption efficiency of CR, AR1, and RR2 dye molecules on CAG spherical beads were 34.89, 32.36 and 33.63 mg/g, respectively.
32827623	5	4	theme	CR	860:861	arg1	mg/g					942:945	34.89, 32.36 and 33.63 mg/g	919:945	mg/g	942:945	The maximum adsorption efficiency of CR, AR1, and RR2 dye molecules on CAG spherical beads were 34.89, 32.36 and 33.63 mg/g, respectively.
32827623	2	5	theme	spherical	510:518	arg1	beads					520:524	the CAG spherical beads	502:524	the CAG spherical beads	502:524	The surface morphology, specific surface area, crystalline phases, elemental composition and thermal properties of the CAG spherical beads had examined.
32827623	5	6	theme	maximum	827:833	arg1	efficiency					846:855	The maximum adsorption efficiency	823:855	The maximum adsorption efficiency of CR, AR1, and RR2 dye molecules on CAG spherical beads	823:912	The maximum adsorption efficiency of CR, AR1, and RR2 dye molecules on CAG spherical beads were 34.89, 32.36 and 33.63 mg/g, respectively.
32827623	5	6	theme	maximum	827:833	arg1	mg/g					942:945	34.89, 32.36 and 33.63 mg/g	919:945	mg/g	942:945	The maximum adsorption efficiency of CR, AR1, and RR2 dye molecules on CAG spherical beads were 34.89, 32.36 and 33.63 mg/g, respectively.
32827623	4	7	theme	solution	690:697	arg1	pH					699:700	solution pH	690:700	solution pH	690:700	The adsorption parameters like solution pH, contact time, co-existing ions, adsorbent dosage and regeneration studies were optimized using batch experiment method.
32827623	5	8	theme	dye	877:879	arg1	molecules					881:889	RR2 dye molecules	873:889	RR2 dye molecules	873:889	The maximum adsorption efficiency of CR, AR1, and RR2 dye molecules on CAG spherical beads were 34.89, 32.36 and 33.63 mg/g, respectively.
32827623	8	9	theme	spherical	1281:1289	arg1	beads					1291:1295	The CAG spherical beads	1273:1295	The CAG spherical beads	1273:1295	The CAG spherical beads could be regenerated up to six consecutive cycles using an aqueous 0.1 M NaOH solution.
32827623	8	10	theme	aqueous	1356:1362	arg1	solution					1375:1382	an aqueous 0.1 M NaOH solution	1353:1382	an aqueous 0.1 M NaOH solution	1353:1382	The CAG spherical beads could be regenerated up to six consecutive cycles using an aqueous 0.1 M NaOH solution.
32827623	3	11	theme	adsorption	596:605	arg1	properties					607:616	the adsorption properties	592:616	the adsorption properties of dye molecules on CAG spherical beads	592:656	Adsorption experiments were explored to investigate the adsorption properties of dye molecules on CAG spherical beads.
32827623	6	12	theme	adsorption	966:975	arg1	system					977:982	The adsorption system	962:982	The adsorption system	962:982	The adsorption system fits well with pseudo-second-order kinetics and Freundlich isotherm model.
32827623	2	13	theme	elemental	454:462	arg1	composition					464:474	elemental composition	454:474	elemental composition	454:474	The surface morphology, specific surface area, crystalline phases, elemental composition and thermal properties of the CAG spherical beads had examined.
32827623	9	14	theme	water/wastewater	1488:1503	arg1	process					1515:1521	the water/wastewater treatment process	1484:1521	the water/wastewater treatment process	1484:1521	The study emphasizes that the fabricated CAG spherical beads could act as a potential adsorbent in the water/wastewater treatment process.
32827623	7	15	theme	bondings	1202:1209	arg1	force					1147:1151	the electrostatic force	1129:1151	the electrostatic force of attraction, surface complexation and hydrogen bondings that exist between dye molecules and the CAG spherical beads	1129:1270	The molecular interactions followed in the adsorption mechanisms were the electrostatic force of attraction, surface complexation and hydrogen bondings that exist between dye molecules and the CAG spherical beads.
32827623	7	15	theme	bondings	1202:1209	arg1	interactions					1073:1084	The molecular interactions	1059:1084	The molecular interactions followed in the adsorption mechanisms	1059:1122	The molecular interactions followed in the adsorption mechanisms were the electrostatic force of attraction, surface complexation and hydrogen bondings that exist between dye molecules and the CAG spherical beads.
32827623	5	16	theme	AR1	864:866	arg1	efficiency					846:855	The maximum adsorption efficiency	823:855	The maximum adsorption efficiency of CR, AR1, and RR2 dye molecules on CAG spherical beads	823:912	The maximum adsorption efficiency of CR, AR1, and RR2 dye molecules on CAG spherical beads were 34.89, 32.36 and 33.63 mg/g, respectively.
32827623	5	16	theme	AR1	864:866	arg1	mg/g					942:945	34.89, 32.36 and 33.63 mg/g	919:945	mg/g	942:945	The maximum adsorption efficiency of CR, AR1, and RR2 dye molecules on CAG spherical beads were 34.89, 32.36 and 33.63 mg/g, respectively.
32827623	0	17	theme	organic	100:106	arg1	pollutants					108:117	organic pollutants	100:117	organic pollutants	100:117	Environment responsive Al3+ networked chitosan-gelatin spherical beads for the effective removal of organic pollutants from aqueous solutions.
32827623	1	18	theme	spherical	298:306	arg1	chitosan-gelatin					308:323	biodegradable spherical chitosan-gelatin	284:323	biodegradable spherical chitosan-gelatin	284:323	In order to remove noxious Congo Red (CR), Acid Red 1 (AR1) and Reactive Red 2 (RR2) dye molecules from water, an environment responsive and biodegradable spherical chitosan-gelatin biopolymeric beads were designed and embedded with Al3+ ions.
32827623	8	19	theme	consecutive	1328:1338	arg1	cycles					1340:1345	six consecutive cycles	1324:1345	six consecutive cycles using an aqueous 0.1 M NaOH solution	1324:1382	The CAG spherical beads could be regenerated up to six consecutive cycles using an aqueous 0.1 M NaOH solution.
32827623	7	20	theme	surface	1168:1174	arg1	complexation					1176:1187	surface complexation	1168:1187	surface complexation	1168:1187	The molecular interactions followed in the adsorption mechanisms were the electrostatic force of attraction, surface complexation and hydrogen bondings that exist between dye molecules and the CAG spherical beads.
32827623	1	21	theme	biopolymeric	325:336	arg1	beads					338:342	biopolymeric beads	325:342	biopolymeric beads	325:342	In order to remove noxious Congo Red (CR), Acid Red 1 (AR1) and Reactive Red 2 (RR2) dye molecules from water, an environment responsive and biodegradable spherical chitosan-gelatin biopolymeric beads were designed and embedded with Al3+ ions.
32827623	6	22	theme	Freundlich	1032:1041	arg1	model					1052:1056	Freundlich isotherm model	1032:1056	Freundlich isotherm model	1032:1056	The adsorption system fits well with pseudo-second-order kinetics and Freundlich isotherm model.
32827623	1	23	dep	water	247:251	arg1	beads					338:342	biopolymeric beads	325:342	biopolymeric beads	325:342	In order to remove noxious Congo Red (CR), Acid Red 1 (AR1) and Reactive Red 2 (RR2) dye molecules from water, an environment responsive and biodegradable spherical chitosan-gelatin biopolymeric beads were designed and embedded with Al3+ ions.
32827623	7	24	theme	attraction	1156:1165	arg1	force					1147:1151	the electrostatic force	1129:1151	the electrostatic force of attraction, surface complexation and hydrogen bondings that exist between dye molecules and the CAG spherical beads	1129:1270	The molecular interactions followed in the adsorption mechanisms were the electrostatic force of attraction, surface complexation and hydrogen bondings that exist between dye molecules and the CAG spherical beads.
32827623	7	24	theme	attraction	1156:1165	arg1	interactions					1073:1084	The molecular interactions	1059:1084	The molecular interactions followed in the adsorption mechanisms	1059:1122	The molecular interactions followed in the adsorption mechanisms were the electrostatic force of attraction, surface complexation and hydrogen bondings that exist between dye molecules and the CAG spherical beads.
32827623	2	25	dep	morphology	399:408	arg1	The					387:389	The	387:389	The	387:389	The surface morphology, specific surface area, crystalline phases, elemental composition and thermal properties of the CAG spherical beads had examined.
32827623	5	26	theme	spherical	898:906	arg1	beads					908:912	CAG spherical beads	894:912	CAG spherical beads	894:912	The maximum adsorption efficiency of CR, AR1, and RR2 dye molecules on CAG spherical beads were 34.89, 32.36 and 33.63 mg/g, respectively.
32827623	2	27	theme	surface	391:397	arg1	morphology					399:408	surface morphology	391:408	surface morphology	391:408	The surface morphology, specific surface area, crystalline phases, elemental composition and thermal properties of the CAG spherical beads had examined.
32827623	4	28	theme	co-existing	717:727	arg1	ions					729:732	co-existing ions	717:732	co-existing ions	717:732	The adsorption parameters like solution pH, contact time, co-existing ions, adsorbent dosage and regeneration studies were optimized using batch experiment method.
32827623	1	29	from	chitosan-gelatin	308:323	arg1	Acid					186:189	Acid Red 1	186:195	Acid Red 1 (AR1)	186:201	In order to remove noxious Congo Red (CR), Acid Red 1 (AR1) and Reactive Red 2 (RR2) dye molecules from water, an environment responsive and biodegradable spherical chitosan-gelatin biopolymeric beads were designed and embedded with Al3+ ions.
32827623	1	29	from	chitosan-gelatin	308:323	arg1	AR1					198:200	AR1	198:200	AR1	198:200	In order to remove noxious Congo Red (CR), Acid Red 1 (AR1) and Reactive Red 2 (RR2) dye molecules from water, an environment responsive and biodegradable spherical chitosan-gelatin biopolymeric beads were designed and embedded with Al3+ ions.
32827623	1	30	theme	dye	228:230	arg1	molecules					232:240	dye molecules	228:240	dye molecules	228:240	In order to remove noxious Congo Red (CR), Acid Red 1 (AR1) and Reactive Red 2 (RR2) dye molecules from water, an environment responsive and biodegradable spherical chitosan-gelatin biopolymeric beads were designed and embedded with Al3+ ions.
32827623	3	31	theme	molecules	625:633	arg1	properties					607:616	the adsorption properties	592:616	the adsorption properties of dye molecules on CAG spherical beads	592:656	Adsorption experiments were explored to investigate the adsorption properties of dye molecules on CAG spherical beads.
32827623	8	32	theme	NaOH	1370:1373	arg1	solution					1375:1382	an aqueous 0.1 M NaOH solution	1353:1382	an aqueous 0.1 M NaOH solution	1353:1382	The CAG spherical beads could be regenerated up to six consecutive cycles using an aqueous 0.1 M NaOH solution.
32827623	0	33	theme	chitosan-gelatin	38:53	arg1	beads					65:69	chitosan-gelatin spherical beads	38:69	chitosan-gelatin spherical beads	38:69	Environment responsive Al3+ networked chitosan-gelatin spherical beads for the effective removal of organic pollutants from aqueous solutions.
32827623	2	34	theme	surface	420:426	arg1	area					428:431	specific surface area	411:431	specific surface area	411:431	The surface morphology, specific surface area, crystalline phases, elemental composition and thermal properties of the CAG spherical beads had examined.
32827623	4	35	theme	experiment	804:813	arg1	method					815:820	batch experiment method	798:820	batch experiment method	798:820	The adsorption parameters like solution pH, contact time, co-existing ions, adsorbent dosage and regeneration studies were optimized using batch experiment method.
32827623	4	36	theme	regeneration	756:767	arg1	studies					769:775	regeneration studies	756:775	regeneration studies	756:775	The adsorption parameters like solution pH, contact time, co-existing ions, adsorbent dosage and regeneration studies were optimized using batch experiment method.
32827623	7	37	theme	CAG	1252:1254	arg1	beads					1266:1270	the CAG spherical beads	1248:1270	the CAG spherical beads	1248:1270	The molecular interactions followed in the adsorption mechanisms were the electrostatic force of attraction, surface complexation and hydrogen bondings that exist between dye molecules and the CAG spherical beads.
32827623	3	38	theme	CAG	638:640	arg1	beads					652:656	CAG spherical beads	638:656	CAG spherical beads	638:656	Adsorption experiments were explored to investigate the adsorption properties of dye molecules on CAG spherical beads.
32827623	1	39	from	environment	257:267	arg1	Acid					186:189	Acid Red 1	186:195	Acid Red 1 (AR1)	186:201	In order to remove noxious Congo Red (CR), Acid Red 1 (AR1) and Reactive Red 2 (RR2) dye molecules from water, an environment responsive and biodegradable spherical chitosan-gelatin biopolymeric beads were designed and embedded with Al3+ ions.
32827623	1	39	from	environment	257:267	arg1	AR1					198:200	AR1	198:200	AR1	198:200	In order to remove noxious Congo Red (CR), Acid Red 1 (AR1) and Reactive Red 2 (RR2) dye molecules from water, an environment responsive and biodegradable spherical chitosan-gelatin biopolymeric beads were designed and embedded with Al3+ ions.
32827623	0	40	from	solutions	132:140	arg1	removal					89:95	the effective removal	75:95	the effective removal of organic pollutants from aqueous solutions	75:140	Environment responsive Al3+ networked chitosan-gelatin spherical beads for the effective removal of organic pollutants from aqueous solutions.
32827623	7	41	theme	dye	1230:1232	arg1	molecules					1234:1242	dye molecules	1230:1242	dye molecules	1230:1242	The molecular interactions followed in the adsorption mechanisms were the electrostatic force of attraction, surface complexation and hydrogen bondings that exist between dye molecules and the CAG spherical beads.
32827623	3	42	from	properties	607:616	arg1	beads					652:656	CAG spherical beads	638:656	CAG spherical beads	638:656	Adsorption experiments were explored to investigate the adsorption properties of dye molecules on CAG spherical beads.
32827623	5	43	theme	adsorption	835:844	arg1	efficiency					846:855	The maximum adsorption efficiency	823:855	The maximum adsorption efficiency of CR, AR1, and RR2 dye molecules on CAG spherical beads	823:912	The maximum adsorption efficiency of CR, AR1, and RR2 dye molecules on CAG spherical beads were 34.89, 32.36 and 33.63 mg/g, respectively.
32827623	5	43	theme	adsorption	835:844	arg1	mg/g					942:945	34.89, 32.36 and 33.63 mg/g	919:945	mg/g	942:945	The maximum adsorption efficiency of CR, AR1, and RR2 dye molecules on CAG spherical beads were 34.89, 32.36 and 33.63 mg/g, respectively.
32827623	9	44	from	adsorbent	1471:1479	arg1	process					1515:1521	the water/wastewater treatment process	1484:1521	the water/wastewater treatment process	1484:1521	The study emphasizes that the fabricated CAG spherical beads could act as a potential adsorbent in the water/wastewater treatment process.
32827623	9	45	theme	CAG	1426:1428	arg1	adsorbent					1471:1479	adsorbent	1471:1479	adsorbent	1471:1479	The study emphasizes that the fabricated CAG spherical beads could act as a potential adsorbent in the water/wastewater treatment process.
32827623	9	45	theme	CAG	1426:1428	arg1	beads					1440:1444	the fabricated CAG spherical beads	1411:1444	the fabricated CAG spherical beads	1411:1444	The study emphasizes that the fabricated CAG spherical beads could act as a potential adsorbent in the water/wastewater treatment process.
32827623	2	46	theme	CAG	506:508	arg1	beads					520:524	the CAG spherical beads	502:524	the CAG spherical beads	502:524	The surface morphology, specific surface area, crystalline phases, elemental composition and thermal properties of the CAG spherical beads had examined.
32827623	9	47	theme	spherical	1430:1438	arg1	adsorbent					1471:1479	adsorbent	1471:1479	adsorbent	1471:1479	The study emphasizes that the fabricated CAG spherical beads could act as a potential adsorbent in the water/wastewater treatment process.
32827623	9	47	theme	spherical	1430:1438	arg1	beads					1440:1444	the fabricated CAG spherical beads	1411:1444	the fabricated CAG spherical beads	1411:1444	The study emphasizes that the fabricated CAG spherical beads could act as a potential adsorbent in the water/wastewater treatment process.
32827623	1	48	theme	Red	191:193	arg1	Acid					186:189	Acid Red 1	186:195	Acid Red 1 (AR1)	186:201	In order to remove noxious Congo Red (CR), Acid Red 1 (AR1) and Reactive Red 2 (RR2) dye molecules from water, an environment responsive and biodegradable spherical chitosan-gelatin biopolymeric beads were designed and embedded with Al3+ ions.
32827623	1	48	theme	Red	191:193	arg1	AR1					198:200	AR1	198:200	AR1	198:200	In order to remove noxious Congo Red (CR), Acid Red 1 (AR1) and Reactive Red 2 (RR2) dye molecules from water, an environment responsive and biodegradable spherical chitosan-gelatin biopolymeric beads were designed and embedded with Al3+ ions.
32827623	7	49	theme	complexation	1176:1187	arg1	force					1147:1151	the electrostatic force	1129:1151	the electrostatic force of attraction, surface complexation and hydrogen bondings that exist between dye molecules and the CAG spherical beads	1129:1270	The molecular interactions followed in the adsorption mechanisms were the electrostatic force of attraction, surface complexation and hydrogen bondings that exist between dye molecules and the CAG spherical beads.
32827623	7	49	theme	complexation	1176:1187	arg1	interactions					1073:1084	The molecular interactions	1059:1084	The molecular interactions followed in the adsorption mechanisms	1059:1122	The molecular interactions followed in the adsorption mechanisms were the electrostatic force of attraction, surface complexation and hydrogen bondings that exist between dye molecules and the CAG spherical beads.
32827623	3	50	theme	Adsorption	540:549	arg1	experiments					551:561	Adsorption experiments	540:561	Adsorption experiments	540:561	Adsorption experiments were explored to investigate the adsorption properties of dye molecules on CAG spherical beads.
32827623	5	51	theme	RR2	873:875	arg1	molecules					881:889	RR2 dye molecules	873:889	RR2 dye molecules	873:889	The maximum adsorption efficiency of CR, AR1, and RR2 dye molecules on CAG spherical beads were 34.89, 32.36 and 33.63 mg/g, respectively.
32827623	1	52	theme	Al3+	376:379	arg1	ions					381:384	Al3+ ions	376:384	Al3+ ions	376:384	In order to remove noxious Congo Red (CR), Acid Red 1 (AR1) and Reactive Red 2 (RR2) dye molecules from water, an environment responsive and biodegradable spherical chitosan-gelatin biopolymeric beads were designed and embedded with Al3+ ions.
32827623	5	53	theme	molecules	881:889	arg1	efficiency					846:855	The maximum adsorption efficiency	823:855	The maximum adsorption efficiency of CR, AR1, and RR2 dye molecules on CAG spherical beads	823:912	The maximum adsorption efficiency of CR, AR1, and RR2 dye molecules on CAG spherical beads were 34.89, 32.36 and 33.63 mg/g, respectively.
32827623	5	53	theme	molecules	881:889	arg1	mg/g					942:945	34.89, 32.36 and 33.63 mg/g	919:945	mg/g	942:945	The maximum adsorption efficiency of CR, AR1, and RR2 dye molecules on CAG spherical beads were 34.89, 32.36 and 33.63 mg/g, respectively.
32827623	2	54	theme	beads	520:524	arg1	morphology					399:408	surface morphology	391:408	surface morphology	391:408	The surface morphology, specific surface area, crystalline phases, elemental composition and thermal properties of the CAG spherical beads had examined.
32827623	2	54	theme	beads	520:524	arg1	area					428:431	specific surface area	411:431	specific surface area	411:431	The surface morphology, specific surface area, crystalline phases, elemental composition and thermal properties of the CAG spherical beads had examined.
32827623	2	54	theme	beads	520:524	arg1	phases					446:451	crystalline phases	434:451	crystalline phases	434:451	The surface morphology, specific surface area, crystalline phases, elemental composition and thermal properties of the CAG spherical beads had examined.
32827623	2	54	theme	beads	520:524	arg1	composition					464:474	elemental composition	454:474	elemental composition	454:474	The surface morphology, specific surface area, crystalline phases, elemental composition and thermal properties of the CAG spherical beads had examined.
32827623	2	54	theme	beads	520:524	arg1	properties					488:497	thermal properties	480:497	thermal properties	480:497	The surface morphology, specific surface area, crystalline phases, elemental composition and thermal properties of the CAG spherical beads had examined.
32827623	8	55	theme	CAG	1277:1279	arg1	beads					1291:1295	The CAG spherical beads	1273:1295	The CAG spherical beads	1273:1295	The CAG spherical beads could be regenerated up to six consecutive cycles using an aqueous 0.1 M NaOH solution.
32827623	0	56	theme	effective	79:87	arg1	removal					89:95	the effective removal	75:95	the effective removal of organic pollutants from aqueous solutions	75:140	Environment responsive Al3+ networked chitosan-gelatin spherical beads for the effective removal of organic pollutants from aqueous solutions.
32827623	7	57	theme	hydrogen	1193:1200	arg1	bondings					1202:1209	hydrogen bondings	1193:1209	hydrogen bondings	1193:1209	The molecular interactions followed in the adsorption mechanisms were the electrostatic force of attraction, surface complexation and hydrogen bondings that exist between dye molecules and the CAG spherical beads.
32827623	1	58	theme	responsive	269:278	arg1	environment					257:267	an environment responsive	254:278	an environment responsive	254:278	In order to remove noxious Congo Red (CR), Acid Red 1 (AR1) and Reactive Red 2 (RR2) dye molecules from water, an environment responsive and biodegradable spherical chitosan-gelatin biopolymeric beads were designed and embedded with Al3+ ions.
32827623	5	59	from	efficiency	846:855	arg1	beads					908:912	CAG spherical beads	894:912	CAG spherical beads	894:912	The maximum adsorption efficiency of CR, AR1, and RR2 dye molecules on CAG spherical beads were 34.89, 32.36 and 33.63 mg/g, respectively.
32827623	9	60	theme	treatment	1505:1513	arg1	process					1515:1521	the water/wastewater treatment process	1484:1521	the water/wastewater treatment process	1484:1521	The study emphasizes that the fabricated CAG spherical beads could act as a potential adsorbent in the water/wastewater treatment process.
32827623	2	61	theme	crystalline	434:444	arg1	phases					446:451	crystalline phases	434:451	crystalline phases	434:451	The surface morphology, specific surface area, crystalline phases, elemental composition and thermal properties of the CAG spherical beads had examined.
32827623	4	62	theme	adsorption	663:672	arg1	parameters					674:683	The adsorption parameters	659:683	The adsorption parameters like solution pH, contact time, co-existing ions, adsorbent dosage and regeneration studies	659:775	The adsorption parameters like solution pH, contact time, co-existing ions, adsorbent dosage and regeneration studies were optimized using batch experiment method.
32827623	0	63	theme	pollutants	108:117	arg1	removal					89:95	the effective removal	75:95	the effective removal of organic pollutants from aqueous solutions	75:140	Environment responsive Al3+ networked chitosan-gelatin spherical beads for the effective removal of organic pollutants from aqueous solutions.
32827623	1	64	theme	biodegradable	284:296	arg1	chitosan-gelatin					308:323	biodegradable spherical chitosan-gelatin	284:323	biodegradable spherical chitosan-gelatin	284:323	In order to remove noxious Congo Red (CR), Acid Red 1 (AR1) and Reactive Red 2 (RR2) dye molecules from water, an environment responsive and biodegradable spherical chitosan-gelatin biopolymeric beads were designed and embedded with Al3+ ions.
32827623	5	65	theme	34.89	919:923	arg1	efficiency					846:855	The maximum adsorption efficiency	823:855	The maximum adsorption efficiency of CR, AR1, and RR2 dye molecules on CAG spherical beads	823:912	The maximum adsorption efficiency of CR, AR1, and RR2 dye molecules on CAG spherical beads were 34.89, 32.36 and 33.63 mg/g, respectively.
32827623	5	65	theme	34.89	919:923	arg1	mg/g					942:945	34.89, 32.36 and 33.63 mg/g	919:945	mg/g	942:945	The maximum adsorption efficiency of CR, AR1, and RR2 dye molecules on CAG spherical beads were 34.89, 32.36 and 33.63 mg/g, respectively.
32827623	2	66	theme	thermal	480:486	arg1	properties					488:497	thermal properties	480:497	thermal properties	480:497	The surface morphology, specific surface area, crystalline phases, elemental composition and thermal properties of the CAG spherical beads had examined.
32827623	0	67	theme	aqueous	124:130	arg1	solutions					132:140	aqueous solutions	124:140	aqueous solutions	124:140	Environment responsive Al3+ networked chitosan-gelatin spherical beads for the effective removal of organic pollutants from aqueous solutions.
32827623	6	68	theme	isotherm	1043:1050	arg1	model					1052:1056	Freundlich isotherm model	1032:1056	Freundlich isotherm model	1032:1056	The adsorption system fits well with pseudo-second-order kinetics and Freundlich isotherm model.
32827623	4	69	theme	adsorbent	735:743	arg1	dosage					745:750	adsorbent dosage	735:750	adsorbent dosage	735:750	The adsorption parameters like solution pH, contact time, co-existing ions, adsorbent dosage and regeneration studies were optimized using batch experiment method.
32827623	0	70	theme	responsive	12:21	arg1	Al3+					23:26	Environment responsive Al3+	0:26	Environment responsive Al3+	0:26	Environment responsive Al3+ networked chitosan-gelatin spherical beads for the effective removal of organic pollutants from aqueous solutions.
32827623	5	71	theme	CAG	894:896	arg1	beads					908:912	CAG spherical beads	894:912	CAG spherical beads	894:912	The maximum adsorption efficiency of CR, AR1, and RR2 dye molecules on CAG spherical beads were 34.89, 32.36 and 33.63 mg/g, respectively.
32827623	4	72	theme	contact	703:709	arg1	time					711:714	contact time	703:714	contact time	703:714	The adsorption parameters like solution pH, contact time, co-existing ions, adsorbent dosage and regeneration studies were optimized using batch experiment method.
32827623	7	73	theme	electrostatic	1133:1145	arg1	force					1147:1151	the electrostatic force	1129:1151	the electrostatic force of attraction, surface complexation and hydrogen bondings that exist between dye molecules and the CAG spherical beads	1129:1270	The molecular interactions followed in the adsorption mechanisms were the electrostatic force of attraction, surface complexation and hydrogen bondings that exist between dye molecules and the CAG spherical beads.
32827623	7	73	theme	electrostatic	1133:1145	arg1	interactions					1073:1084	The molecular interactions	1059:1084	The molecular interactions followed in the adsorption mechanisms	1059:1122	The molecular interactions followed in the adsorption mechanisms were the electrostatic force of attraction, surface complexation and hydrogen bondings that exist between dye molecules and the CAG spherical beads.
32827623	1	74	with	embedded	362:369	arg1	ions					381:384	Al3+ ions	376:384	Al3+ ions	376:384	In order to remove noxious Congo Red (CR), Acid Red 1 (AR1) and Reactive Red 2 (RR2) dye molecules from water, an environment responsive and biodegradable spherical chitosan-gelatin biopolymeric beads were designed and embedded with Al3+ ions.
32827623	6	75	theme	pseudo-second-order	999:1017	arg1	kinetics					1019:1026	pseudo-second-order kinetics	999:1026	pseudo-second-order kinetics	999:1026	The adsorption system fits well with pseudo-second-order kinetics and Freundlich isotherm model.
32827623	3	76	theme	dye	621:623	arg1	molecules					625:633	dye molecules	621:633	dye molecules	621:633	Adsorption experiments were explored to investigate the adsorption properties of dye molecules on CAG spherical beads.
32827623	0	77	theme	spherical	55:63	arg1	beads					65:69	chitosan-gelatin spherical beads	38:69	chitosan-gelatin spherical beads	38:69	Environment responsive Al3+ networked chitosan-gelatin spherical beads for the effective removal of organic pollutants from aqueous solutions.
32827623	8	78	theme	0.1 M	1364:1368	arg1	solution					1375:1382	an aqueous 0.1 M NaOH solution	1353:1382	an aqueous 0.1 M NaOH solution	1353:1382	The CAG spherical beads could be regenerated up to six consecutive cycles using an aqueous 0.1 M NaOH solution.
32827623	7	79	theme	adsorption	1102:1111	arg1	mechanisms					1113:1122	the adsorption mechanisms	1098:1122	the adsorption mechanisms	1098:1122	The molecular interactions followed in the adsorption mechanisms were the electrostatic force of attraction, surface complexation and hydrogen bondings that exist between dye molecules and the CAG spherical beads.
32827623	7	80	theme	spherical	1256:1264	arg1	beads					1266:1270	the CAG spherical beads	1248:1270	the CAG spherical beads	1248:1270	The molecular interactions followed in the adsorption mechanisms were the electrostatic force of attraction, surface complexation and hydrogen bondings that exist between dye molecules and the CAG spherical beads.
32827623	2	81	theme	specific	411:418	arg1	area					428:431	specific surface area	411:431	specific surface area	411:431	The surface morphology, specific surface area, crystalline phases, elemental composition and thermal properties of the CAG spherical beads had examined.
32827623	3	82	theme	spherical	642:650	arg1	beads					652:656	CAG spherical beads	638:656	CAG spherical beads	638:656	Adsorption experiments were explored to investigate the adsorption properties of dye molecules on CAG spherical beads.
32827623	4	83	theme	batch	798:802	arg1	method					815:820	batch experiment method	798:820	batch experiment method	798:820	The adsorption parameters like solution pH, contact time, co-existing ions, adsorbent dosage and regeneration studies were optimized using batch experiment method.
32827623	5	84	theme	33.63	936:940	arg1	efficiency					846:855	The maximum adsorption efficiency	823:855	The maximum adsorption efficiency of CR, AR1, and RR2 dye molecules on CAG spherical beads	823:912	The maximum adsorption efficiency of CR, AR1, and RR2 dye molecules on CAG spherical beads were 34.89, 32.36 and 33.63 mg/g, respectively.
32827623	5	84	theme	33.63	936:940	arg1	mg/g					942:945	34.89, 32.36 and 33.63 mg/g	919:945	mg/g	942:945	The maximum adsorption efficiency of CR, AR1, and RR2 dye molecules on CAG spherical beads were 34.89, 32.36 and 33.63 mg/g, respectively.
32827623	1	85	dep	Acid	186:189	arg1	molecules					232:240	dye molecules	228:240	dye molecules	228:240	In order to remove noxious Congo Red (CR), Acid Red 1 (AR1) and Reactive Red 2 (RR2) dye molecules from water, an environment responsive and biodegradable spherical chitosan-gelatin biopolymeric beads were designed and embedded with Al3+ ions.
31792076	11	0	theme	PE	1560:1561	arg1	synthesis					1563:1571	decreased PE synthesis	1550:1571	decreased PE synthesis	1550:1571	In contrast to mutants with decreased PE synthesis, there were no transcriptional differences between the EPT1 overexpression strain and the wild type, indicating that the hypervirulent phenotype is a consequence of posttranscriptional changes.
31792076	9	1	dep	cause	1250:1254	arg1	reflect					1329:1335	reflect	1329:1335	may reflect the substantial cell wall defects in the psd1Δ/Δ psd2Δ/Δ mutant	1325:1399	Losses of PSD1 and PSD2, but not EPT1, cause significant increases in transcription of glycosylation genes, which may reflect the substantial cell wall defects in the psd1Δ/Δ psd2Δ/Δ mutant.
31792076	8	2	theme	mitochondrial	1187:1199	arg1	function					1201:1208	mitochondrial function	1187:1208	mitochondrial function	1187:1208	Decreased PE synthesis from loss of EPT1 or PSD1 and PSD2 leads to downregulation of genes that impact mitochondrial function.
31792076	12	3	theme	EPT1	1803:1806	arg1	overexpression					1785:1798	overexpression	1785:1798	overexpression of EPT1	1785:1806	It was found that overexpression of EPT1 causes increased chitin content and increased hyphal length.
31792076	9	4	theme	PSD1	1221:1224	arg1	Losses					1211:1216	Losses	1211:1216	Losses of PSD1 and PSD2	1211:1233	Losses of PSD1 and PSD2, but not EPT1, cause significant increases in transcription of glycosylation genes, which may reflect the substantial cell wall defects in the psd1Δ/Δ psd2Δ/Δ mutant.
31792076	9	5	theme	PSD2	1230:1233	arg1	Losses					1211:1216	Losses	1211:1216	Losses of PSD1 and PSD2	1211:1233	Losses of PSD1 and PSD2, but not EPT1, cause significant increases in transcription of glycosylation genes, which may reflect the substantial cell wall defects in the psd1Δ/Δ psd2Δ/Δ mutant.
31792076	2	6	theme	psd2Δ/Δ	349:355	arg1	avirulent					457:465	avirulent	457:465	avirulent	457:465	The psd1Δ/Δ psd2Δ/Δ mutant, which cannot synthesize PE by the cytidine diphosphate diacylglycerol (CDP-DAG) pathway, is avirulent in the mouse model of systemic candidiasis.
31792076	2	6	theme	psd2Δ/Δ	349:355	arg1	mutant					357:362	The psd1Δ/Δ psd2Δ/Δ mutant	337:362	The psd1Δ/Δ psd2Δ/Δ mutant	337:362	The psd1Δ/Δ psd2Δ/Δ mutant, which cannot synthesize PE by the cytidine diphosphate diacylglycerol (CDP-DAG) pathway, is avirulent in the mouse model of systemic candidiasis.
31792076	1	7	theme	bloodstream	222:232	arg1	infections					234:243	systemic bloodstream infections	213:243	systemic bloodstream infections	213:243	Candida albicans is a leading cause of systemic bloodstream infections, and synthesis of the phospholipid phosphatidylethanolamine (PE) is required for virulence.
31792076	0	8	theme	Posttranscriptional	142:160	arg1	Mechanisms					162:171	Transcriptional and Posttranscriptional Mechanisms	122:171	Transcriptional and Posttranscriptional Mechanisms	122:171	Pathways That Synthesize Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition through Transcriptional and Posttranscriptional Mechanisms.
31792076	3	9	theme	Kennedy	572:578	arg1	pathway					580:586	the Kennedy pathway	568:586	the Kennedy pathway	568:586	Similarly, an ept1Δ/Δ mutant, which cannot produce PE by the Kennedy pathway, exhibits decreased kidney fungal burden in systemically infected mice.
31792076	5	10	theme	PE	838:839	arg1	synthesis					841:849	PE synthesis	838:849	PE synthesis	838:849	Thus, mutations that increase PE synthesis increase virulence, and mutations that decrease PE synthesis decrease virulence.
31792076	7	11	theme	PE	1040:1041	arg1	biosynthesis					1043:1054	deficient or excessive PE biosynthesis	1017:1054	deficient or excessive PE biosynthesis	1017:1054	RNA sequencing was performed on strains with deficient or excessive PE biosynthesis to elucidate the mechanism.
31792076	3	12	theme	decreased	598:606	arg1	burden					622:627	decreased kidney fungal burden	598:627	decreased kidney fungal burden in systemically infected mice	598:657	Similarly, an ept1Δ/Δ mutant, which cannot produce PE by the Kennedy pathway, exhibits decreased kidney fungal burden in systemically infected mice.
31792076	13	13	theme	overexpressor	1957:1969	arg1	EPT1					1952:1955	the EPT1 overexpressor	1948:1969	the EPT1 overexpressor	1948:1969	These phenotypes may help to explain the previously observed hypervirulence in the EPT1 overexpressor.
31792076	3	14	theme	infected	645:652	arg1	mice					654:657	systemically infected mice	632:657	systemically infected mice	632:657	Similarly, an ept1Δ/Δ mutant, which cannot produce PE by the Kennedy pathway, exhibits decreased kidney fungal burden in systemically infected mice.
31792076	1	15	theme	Candida	174:180	arg1	cause					204:208	a leading cause	194:208	a leading cause of systemic bloodstream infections	194:243	Candida albicans is a leading cause of systemic bloodstream infections, and synthesis of the phospholipid phosphatidylethanolamine (PE) is required for virulence.
31792076	1	15	theme	Candida	174:180	arg1	albicans					182:189	Candida albicans	174:189	Candida albicans	174:189	Candida albicans is a leading cause of systemic bloodstream infections, and synthesis of the phospholipid phosphatidylethanolamine (PE) is required for virulence.
31792076	7	16	with	strains	1004:1010	arg1	biosynthesis					1043:1054	deficient or excessive PE biosynthesis	1017:1054	deficient or excessive PE biosynthesis	1017:1054	RNA sequencing was performed on strains with deficient or excessive PE biosynthesis to elucidate the mechanism.
31792076	5	17	theme	PE	777:778	arg1	synthesis					780:788	PE synthesis	777:788	PE synthesis	777:788	Thus, mutations that increase PE synthesis increase virulence, and mutations that decrease PE synthesis decrease virulence.
31792076	2	18	from	avirulent	457:465	arg1	model					480:484	the mouse model	470:484	the mouse model of systemic candidiasis	470:508	The psd1Δ/Δ psd2Δ/Δ mutant, which cannot synthesize PE by the cytidine diphosphate diacylglycerol (CDP-DAG) pathway, is avirulent in the mouse model of systemic candidiasis.
31792076	0	19	theme	Cell	92:95	arg1	albicans					65:72	Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition	25:112	Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition	25:112	Pathways That Synthesize Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition through Transcriptional and Posttranscriptional Mechanisms.
31792076	0	19	theme	Cell	92:95	arg1	Composition					102:112	Cell Wall Composition	92:112	Cell Wall Composition	92:112	Pathways That Synthesize Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition through Transcriptional and Posttranscriptional Mechanisms.
31792076	9	20	theme	glycosylation	1298:1310	arg1	genes					1312:1316	glycosylation genes	1298:1316	glycosylation genes	1298:1316	Losses of PSD1 and PSD2, but not EPT1, cause significant increases in transcription of glycosylation genes, which may reflect the substantial cell wall defects in the psd1Δ/Δ psd2Δ/Δ mutant.
31792076	1	21	theme	leading	196:202	arg1	cause					204:208	a leading cause	194:208	a leading cause of systemic bloodstream infections	194:243	Candida albicans is a leading cause of systemic bloodstream infections, and synthesis of the phospholipid phosphatidylethanolamine (PE) is required for virulence.
31792076	1	21	theme	leading	196:202	arg1	albicans					182:189	Candida albicans	174:189	Candida albicans	174:189	Candida albicans is a leading cause of systemic bloodstream infections, and synthesis of the phospholipid phosphatidylethanolamine (PE) is required for virulence.
31792076	11	22	with	mutants	1537:1543	arg1	synthesis					1563:1571	decreased PE synthesis	1550:1571	decreased PE synthesis	1550:1571	In contrast to mutants with decreased PE synthesis, there were no transcriptional differences between the EPT1 overexpression strain and the wild type, indicating that the hypervirulent phenotype is a consequence of posttranscriptional changes.
31792076	7	23	theme	RNA	972:974	arg1	sequencing					976:985	RNA sequencing	972:985	RNA sequencing	972:985	RNA sequencing was performed on strains with deficient or excessive PE biosynthesis to elucidate the mechanism.
31792076	2	24	from	model	480:484	arg1	avirulent					457:465	avirulent	457:465	avirulent	457:465	The psd1Δ/Δ psd2Δ/Δ mutant, which cannot synthesize PE by the cytidine diphosphate diacylglycerol (CDP-DAG) pathway, is avirulent in the mouse model of systemic candidiasis.
31792076	2	24	from	model	480:484	arg1	mutant					357:362	The psd1Δ/Δ psd2Δ/Δ mutant	337:362	The psd1Δ/Δ psd2Δ/Δ mutant	337:362	The psd1Δ/Δ psd2Δ/Δ mutant, which cannot synthesize PE by the cytidine diphosphate diacylglycerol (CDP-DAG) pathway, is avirulent in the mouse model of systemic candidiasis.
31792076	2	25	theme	CDP-DAG	436:442	arg1	pathway					445:451	the cytidine diphosphate diacylglycerol (CDP-DAG) pathway	395:451	the cytidine diphosphate diacylglycerol (CDP-DAG) pathway	395:451	The psd1Δ/Δ psd2Δ/Δ mutant, which cannot synthesize PE by the cytidine diphosphate diacylglycerol (CDP-DAG) pathway, is avirulent in the mouse model of systemic candidiasis.
31792076	0	26	theme	Transcriptional	122:136	arg1	Mechanisms					162:171	Transcriptional and Posttranscriptional Mechanisms	122:171	Transcriptional and Posttranscriptional Mechanisms	122:171	Pathways That Synthesize Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition through Transcriptional and Posttranscriptional Mechanisms.
31792076	13	27	from	hypervirulence	1930:1943	arg1	EPT1					1952:1955	the EPT1 overexpressor	1948:1969	the EPT1 overexpressor	1948:1969	These phenotypes may help to explain the previously observed hypervirulence in the EPT1 overexpressor.
31792076	2	28	theme	mouse	474:478	arg1	model					480:484	the mouse model	470:484	the mouse model of systemic candidiasis	470:508	The psd1Δ/Δ psd2Δ/Δ mutant, which cannot synthesize PE by the cytidine diphosphate diacylglycerol (CDP-DAG) pathway, is avirulent in the mouse model of systemic candidiasis.
31792076	2	29	theme	diacylglycerol	420:433	arg1	pathway					445:451	the cytidine diphosphate diacylglycerol (CDP-DAG) pathway	395:451	the cytidine diphosphate diacylglycerol (CDP-DAG) pathway	395:451	The psd1Δ/Δ psd2Δ/Δ mutant, which cannot synthesize PE by the cytidine diphosphate diacylglycerol (CDP-DAG) pathway, is avirulent in the mouse model of systemic candidiasis.
31792076	8	30	theme	PE	1094:1095	arg1	synthesis					1097:1105	Decreased PE synthesis	1084:1105	Decreased PE synthesis from loss of EPT1 or PSD1 and PSD2	1084:1140	Decreased PE synthesis from loss of EPT1 or PSD1 and PSD2 leads to downregulation of genes that impact mitochondrial function.
31792076	11	31	dep	mutants	1537:1543	arg1	contrast					1525:1532	contrast	1525:1532	contrast	1525:1532	In contrast to mutants with decreased PE synthesis, there were no transcriptional differences between the EPT1 overexpression strain and the wild type, indicating that the hypervirulent phenotype is a consequence of posttranscriptional changes.
31792076	9	32	theme	cell	1353:1356	arg1	defects					1363:1369	the substantial cell wall defects	1337:1369	the substantial cell wall defects in the psd1Δ/Δ psd2Δ/Δ mutant	1337:1399	Losses of PSD1 and PSD2, but not EPT1, cause significant increases in transcription of glycosylation genes, which may reflect the substantial cell wall defects in the psd1Δ/Δ psd2Δ/Δ mutant.
31792076	2	33	theme	candidiasis	498:508	arg1	model					480:484	the mouse model	470:484	the mouse model of systemic candidiasis	470:508	The psd1Δ/Δ psd2Δ/Δ mutant, which cannot synthesize PE by the cytidine diphosphate diacylglycerol (CDP-DAG) pathway, is avirulent in the mouse model of systemic candidiasis.
31792076	10	34	theme	PE	1508:1509	arg1	synthesis					1511:1519	deficient PE synthesis	1498:1519	deficient PE synthesis	1498:1519	These accumulated defects could contribute to the decreased virulence observed for mutants with deficient PE synthesis.
31792076	0	35	theme	Impact	50:55	arg1	Length					81:86	Hyphal Length	74:86	Hyphal Length	74:86	Pathways That Synthesize Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition through Transcriptional and Posttranscriptional Mechanisms.
31792076	0	35	theme	Impact	50:55	arg1	albicans					65:72	Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition	25:112	Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition	25:112	Pathways That Synthesize Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition through Transcriptional and Posttranscriptional Mechanisms.
31792076	0	35	theme	Impact	50:55	arg1	Composition					102:112	Cell Wall Composition	92:112	Cell Wall Composition	92:112	Pathways That Synthesize Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition through Transcriptional and Posttranscriptional Mechanisms.
31792076	0	36	dep	albicans	65:72	arg1	Length					81:86	Hyphal Length	74:86	Hyphal Length	74:86	Pathways That Synthesize Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition through Transcriptional and Posttranscriptional Mechanisms.
31792076	0	36	dep	albicans	65:72	arg1	albicans					65:72	Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition	25:112	Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition	25:112	Pathways That Synthesize Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition through Transcriptional and Posttranscriptional Mechanisms.
31792076	0	36	dep	albicans	65:72	arg1	Composition					102:112	Cell Wall Composition	92:112	Cell Wall Composition	92:112	Pathways That Synthesize Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition through Transcriptional and Posttranscriptional Mechanisms.
31792076	12	37	theme	hyphal	1854:1859	arg1	length					1861:1866	increased hyphal length	1844:1866	increased hyphal length	1844:1866	It was found that overexpression of EPT1 causes increased chitin content and increased hyphal length.
31792076	9	38	theme	psd2Δ/Δ	1386:1392	arg1	mutant					1394:1399	the psd1Δ/Δ psd2Δ/Δ mutant	1374:1399	the psd1Δ/Δ psd2Δ/Δ mutant	1374:1399	Losses of PSD1 and PSD2, but not EPT1, cause significant increases in transcription of glycosylation genes, which may reflect the substantial cell wall defects in the psd1Δ/Δ psd2Δ/Δ mutant.
31792076	12	39	theme	chitin	1825:1830	arg1	content					1832:1838	increased chitin content	1815:1838	increased chitin content	1815:1838	It was found that overexpression of EPT1 causes increased chitin content and increased hyphal length.
31792076	11	40	theme	posttranscriptional	1738:1756	arg1	changes					1758:1764	posttranscriptional changes	1738:1764	posttranscriptional changes	1738:1764	In contrast to mutants with decreased PE synthesis, there were no transcriptional differences between the EPT1 overexpression strain and the wild type, indicating that the hypervirulent phenotype is a consequence of posttranscriptional changes.
31792076	2	41	theme	cytidine	399:406	arg1	pathway					445:451	the cytidine diphosphate diacylglycerol (CDP-DAG) pathway	395:451	the cytidine diphosphate diacylglycerol (CDP-DAG) pathway	395:451	The psd1Δ/Δ psd2Δ/Δ mutant, which cannot synthesize PE by the cytidine diphosphate diacylglycerol (CDP-DAG) pathway, is avirulent in the mouse model of systemic candidiasis.
31792076	1	42	theme	phospholipid	267:278	arg1	PE					306:307	PE	306:307	PE	306:307	Candida albicans is a leading cause of systemic bloodstream infections, and synthesis of the phospholipid phosphatidylethanolamine (PE) is required for virulence.
31792076	1	42	theme	phospholipid	267:278	arg1	phosphatidylethanolamine					280:303	the phospholipid phosphatidylethanolamine	263:303	the phospholipid phosphatidylethanolamine (PE)	263:308	Candida albicans is a leading cause of systemic bloodstream infections, and synthesis of the phospholipid phosphatidylethanolamine (PE) is required for virulence.
31792076	11	43	theme	overexpression	1633:1646	arg1	strain					1648:1653	the EPT1 overexpression strain	1624:1653	the EPT1 overexpression strain	1624:1653	In contrast to mutants with decreased PE synthesis, there were no transcriptional differences between the EPT1 overexpression strain and the wild type, indicating that the hypervirulent phenotype is a consequence of posttranscriptional changes.
31792076	3	44	theme	ept1Δ/Δ	525:531	arg1	mutant					533:538	an ept1Δ/Δ mutant	522:538	an ept1Δ/Δ mutant	522:538	Similarly, an ept1Δ/Δ mutant, which cannot produce PE by the Kennedy pathway, exhibits decreased kidney fungal burden in systemically infected mice.
31792076	6	45	theme	PE	929:930	arg1	synthesis					932:940	PE synthesis	929:940	PE synthesis	929:940	However, the mechanism by which virulence is regulated by PE synthesis is only partially understood.
31792076	11	46	theme	wild	1663:1666	arg1	type					1668:1671	the wild type	1659:1671	the wild type	1659:1671	In contrast to mutants with decreased PE synthesis, there were no transcriptional differences between the EPT1 overexpression strain and the wild type, indicating that the hypervirulent phenotype is a consequence of posttranscriptional changes.
31792076	8	47	theme	genes	1169:1173	arg1	downregulation					1151:1164	downregulation	1151:1164	downregulation of genes that impact mitochondrial function	1151:1208	Decreased PE synthesis from loss of EPT1 or PSD1 and PSD2 leads to downregulation of genes that impact mitochondrial function.
31792076	11	48	theme	hypervirulent	1694:1706	arg1	phenotype					1708:1716	the hypervirulent phenotype	1690:1716	the hypervirulent phenotype	1690:1716	In contrast to mutants with decreased PE synthesis, there were no transcriptional differences between the EPT1 overexpression strain and the wild type, indicating that the hypervirulent phenotype is a consequence of posttranscriptional changes.
31792076	11	48	theme	hypervirulent	1694:1706	arg1	consequence					1723:1733	a consequence	1721:1733	a consequence of posttranscriptional changes	1721:1764	In contrast to mutants with decreased PE synthesis, there were no transcriptional differences between the EPT1 overexpression strain and the wild type, indicating that the hypervirulent phenotype is a consequence of posttranscriptional changes.
31792076	11	49	theme	decreased	1550:1558	arg1	synthesis					1563:1571	decreased PE synthesis	1550:1571	decreased PE synthesis	1550:1571	In contrast to mutants with decreased PE synthesis, there were no transcriptional differences between the EPT1 overexpression strain and the wild type, indicating that the hypervirulent phenotype is a consequence of posttranscriptional changes.
31792076	1	50	theme	systemic	213:220	arg1	infections					234:243	systemic bloodstream infections	213:243	systemic bloodstream infections	213:243	Candida albicans is a leading cause of systemic bloodstream infections, and synthesis of the phospholipid phosphatidylethanolamine (PE) is required for virulence.
31792076	4	51	theme	EPT1	690:693	arg1	overexpression					672:685	overexpression	672:685	overexpression of EPT1	672:693	Conversely, overexpression of EPT1 results in a hypervirulent phenotype in this model.
31792076	10	52	theme	accumulated	1408:1418	arg1	defects					1420:1426	These accumulated defects	1402:1426	These accumulated defects	1402:1426	These accumulated defects could contribute to the decreased virulence observed for mutants with deficient PE synthesis.
31792076	2	53	theme	psd1Δ/Δ	341:347	arg1	avirulent					457:465	avirulent	457:465	avirulent	457:465	The psd1Δ/Δ psd2Δ/Δ mutant, which cannot synthesize PE by the cytidine diphosphate diacylglycerol (CDP-DAG) pathway, is avirulent in the mouse model of systemic candidiasis.
31792076	2	53	theme	psd1Δ/Δ	341:347	arg1	mutant					357:362	The psd1Δ/Δ psd2Δ/Δ mutant	337:362	The psd1Δ/Δ psd2Δ/Δ mutant	337:362	The psd1Δ/Δ psd2Δ/Δ mutant, which cannot synthesize PE by the cytidine diphosphate diacylglycerol (CDP-DAG) pathway, is avirulent in the mouse model of systemic candidiasis.
31792076	1	54	theme	infections	234:243	arg1	cause					204:208	a leading cause	194:208	a leading cause of systemic bloodstream infections	194:243	Candida albicans is a leading cause of systemic bloodstream infections, and synthesis of the phospholipid phosphatidylethanolamine (PE) is required for virulence.
31792076	1	54	theme	infections	234:243	arg1	albicans					182:189	Candida albicans	174:189	Candida albicans	174:189	Candida albicans is a leading cause of systemic bloodstream infections, and synthesis of the phospholipid phosphatidylethanolamine (PE) is required for virulence.
31792076	3	55	theme	kidney	608:613	arg1	burden					622:627	decreased kidney fungal burden	598:627	decreased kidney fungal burden in systemically infected mice	598:657	Similarly, an ept1Δ/Δ mutant, which cannot produce PE by the Kennedy pathway, exhibits decreased kidney fungal burden in systemically infected mice.
31792076	9	56	theme	significant	1256:1266	arg1	increases					1268:1276	significant increases	1256:1276	significant increases in transcription of glycosylation genes	1256:1316	Losses of PSD1 and PSD2, but not EPT1, cause significant increases in transcription of glycosylation genes, which may reflect the substantial cell wall defects in the psd1Δ/Δ psd2Δ/Δ mutant.
31792076	7	57	theme	excessive	1030:1038	arg1	biosynthesis					1043:1054	deficient or excessive PE biosynthesis	1017:1054	deficient or excessive PE biosynthesis	1017:1054	RNA sequencing was performed on strains with deficient or excessive PE biosynthesis to elucidate the mechanism.
31792076	7	58	theme	deficient	1017:1025	arg1	biosynthesis					1043:1054	deficient or excessive PE biosynthesis	1017:1054	deficient or excessive PE biosynthesis	1017:1054	RNA sequencing was performed on strains with deficient or excessive PE biosynthesis to elucidate the mechanism.
31792076	8	59	from	loss	1112:1115	arg1	synthesis					1097:1105	Decreased PE synthesis	1084:1105	Decreased PE synthesis from loss of EPT1 or PSD1 and PSD2	1084:1140	Decreased PE synthesis from loss of EPT1 or PSD1 and PSD2 leads to downregulation of genes that impact mitochondrial function.
31792076	13	60	theme	observed	1921:1928	arg1	hypervirulence					1930:1943	the previously observed hypervirulence	1906:1943	the previously observed hypervirulence in the EPT1 overexpressor	1906:1969	These phenotypes may help to explain the previously observed hypervirulence in the EPT1 overexpressor.
31792076	0	61	theme	Wall	97:100	arg1	albicans					65:72	Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition	25:112	Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition	25:112	Pathways That Synthesize Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition through Transcriptional and Posttranscriptional Mechanisms.
31792076	0	61	theme	Wall	97:100	arg1	Composition					102:112	Cell Wall Composition	92:112	Cell Wall Composition	92:112	Pathways That Synthesize Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition through Transcriptional and Posttranscriptional Mechanisms.
31792076	10	62	theme	decreased	1452:1460	arg1	virulence					1462:1470	the decreased virulence	1448:1470	the decreased virulence observed for mutants with deficient PE synthesis	1448:1519	These accumulated defects could contribute to the decreased virulence observed for mutants with deficient PE synthesis.
31792076	4	63	theme	hypervirulent	708:720	arg1	phenotype					722:730	a hypervirulent phenotype	706:730	a hypervirulent phenotype in this model	706:744	Conversely, overexpression of EPT1 results in a hypervirulent phenotype in this model.
31792076	9	64	theme	genes	1312:1316	arg1	transcription					1281:1293	transcription	1281:1293	transcription of glycosylation genes	1281:1316	Losses of PSD1 and PSD2, but not EPT1, cause significant increases in transcription of glycosylation genes, which may reflect the substantial cell wall defects in the psd1Δ/Δ psd2Δ/Δ mutant.
31792076	9	65	from	defects	1363:1369	arg1	mutant					1394:1399	the psd1Δ/Δ psd2Δ/Δ mutant	1374:1399	the psd1Δ/Δ psd2Δ/Δ mutant	1374:1399	Losses of PSD1 and PSD2, but not EPT1, cause significant increases in transcription of glycosylation genes, which may reflect the substantial cell wall defects in the psd1Δ/Δ psd2Δ/Δ mutant.
31792076	3	66	from	burden	622:627	arg1	mice					654:657	systemically infected mice	632:657	systemically infected mice	632:657	Similarly, an ept1Δ/Δ mutant, which cannot produce PE by the Kennedy pathway, exhibits decreased kidney fungal burden in systemically infected mice.
31792076	8	67	theme	Decreased	1084:1092	arg1	synthesis					1097:1105	Decreased PE synthesis	1084:1105	Decreased PE synthesis from loss of EPT1 or PSD1 and PSD2	1084:1140	Decreased PE synthesis from loss of EPT1 or PSD1 and PSD2 leads to downregulation of genes that impact mitochondrial function.
31792076	2	68	theme	diphosphate	408:418	arg1	pathway					445:451	the cytidine diphosphate diacylglycerol (CDP-DAG) pathway	395:451	the cytidine diphosphate diacylglycerol (CDP-DAG) pathway	395:451	The psd1Δ/Δ psd2Δ/Δ mutant, which cannot synthesize PE by the cytidine diphosphate diacylglycerol (CDP-DAG) pathway, is avirulent in the mouse model of systemic candidiasis.
31792076	0	69	theme	Phosphatidylethanolamine	25:48	arg1	Length					81:86	Hyphal Length	74:86	Hyphal Length	74:86	Pathways That Synthesize Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition through Transcriptional and Posttranscriptional Mechanisms.
31792076	0	69	theme	Phosphatidylethanolamine	25:48	arg1	albicans					65:72	Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition	25:112	Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition	25:112	Pathways That Synthesize Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition through Transcriptional and Posttranscriptional Mechanisms.
31792076	0	69	theme	Phosphatidylethanolamine	25:48	arg1	Composition					102:112	Cell Wall Composition	92:112	Cell Wall Composition	92:112	Pathways That Synthesize Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition through Transcriptional and Posttranscriptional Mechanisms.
31792076	9	70	theme	substantial	1341:1351	arg1	defects					1363:1369	the substantial cell wall defects	1337:1369	the substantial cell wall defects in the psd1Δ/Δ psd2Δ/Δ mutant	1337:1399	Losses of PSD1 and PSD2, but not EPT1, cause significant increases in transcription of glycosylation genes, which may reflect the substantial cell wall defects in the psd1Δ/Δ psd2Δ/Δ mutant.
31792076	0	71	theme	Candida	57:63	arg1	Length					81:86	Hyphal Length	74:86	Hyphal Length	74:86	Pathways That Synthesize Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition through Transcriptional and Posttranscriptional Mechanisms.
31792076	0	71	theme	Candida	57:63	arg1	albicans					65:72	Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition	25:112	Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition	25:112	Pathways That Synthesize Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition through Transcriptional and Posttranscriptional Mechanisms.
31792076	0	71	theme	Candida	57:63	arg1	Composition					102:112	Cell Wall Composition	92:112	Cell Wall Composition	92:112	Pathways That Synthesize Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition through Transcriptional and Posttranscriptional Mechanisms.
31792076	9	72	theme	wall	1358:1361	arg1	defects					1363:1369	the substantial cell wall defects	1337:1369	the substantial cell wall defects in the psd1Δ/Δ psd2Δ/Δ mutant	1337:1399	Losses of PSD1 and PSD2, but not EPT1, cause significant increases in transcription of glycosylation genes, which may reflect the substantial cell wall defects in the psd1Δ/Δ psd2Δ/Δ mutant.
31792076	8	73	theme	EPT1	1120:1123	arg1	loss					1112:1115	loss	1112:1115	loss of EPT1 or PSD1 and PSD2	1112:1140	Decreased PE synthesis from loss of EPT1 or PSD1 and PSD2 leads to downregulation of genes that impact mitochondrial function.
31792076	10	74	with	mutants	1485:1491	arg1	synthesis					1511:1519	deficient PE synthesis	1498:1519	deficient PE synthesis	1498:1519	These accumulated defects could contribute to the decreased virulence observed for mutants with deficient PE synthesis.
31792076	2	75	theme	systemic	489:496	arg1	candidiasis					498:508	systemic candidiasis	489:508	systemic candidiasis	489:508	The psd1Δ/Δ psd2Δ/Δ mutant, which cannot synthesize PE by the cytidine diphosphate diacylglycerol (CDP-DAG) pathway, is avirulent in the mouse model of systemic candidiasis.
31792076	10	76	theme	deficient	1498:1506	arg1	synthesis					1511:1519	deficient PE synthesis	1498:1519	deficient PE synthesis	1498:1519	These accumulated defects could contribute to the decreased virulence observed for mutants with deficient PE synthesis.
31792076	0	77	theme	Hyphal	74:79	arg1	Length					81:86	Hyphal Length	74:86	Hyphal Length	74:86	Pathways That Synthesize Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition through Transcriptional and Posttranscriptional Mechanisms.
31792076	0	77	theme	Hyphal	74:79	arg1	albicans					65:72	Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition	25:112	Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition	25:112	Pathways That Synthesize Phosphatidylethanolamine Impact Candida albicans Hyphal Length and Cell Wall Composition through Transcriptional and Posttranscriptional Mechanisms.
31792076	8	78	theme	PSD1	1128:1131	arg1	loss					1112:1115	loss	1112:1115	loss of EPT1 or PSD1 and PSD2	1112:1140	Decreased PE synthesis from loss of EPT1 or PSD1 and PSD2 leads to downregulation of genes that impact mitochondrial function.
31792076	12	79	theme	increased	1844:1852	arg1	length					1861:1866	increased hyphal length	1844:1866	increased hyphal length	1844:1866	It was found that overexpression of EPT1 causes increased chitin content and increased hyphal length.
31792076	11	80	theme	changes	1758:1764	arg1	phenotype					1708:1716	the hypervirulent phenotype	1690:1716	the hypervirulent phenotype	1690:1716	In contrast to mutants with decreased PE synthesis, there were no transcriptional differences between the EPT1 overexpression strain and the wild type, indicating that the hypervirulent phenotype is a consequence of posttranscriptional changes.
31792076	11	80	theme	changes	1758:1764	arg1	consequence					1723:1733	a consequence	1721:1733	a consequence of posttranscriptional changes	1721:1764	In contrast to mutants with decreased PE synthesis, there were no transcriptional differences between the EPT1 overexpression strain and the wild type, indicating that the hypervirulent phenotype is a consequence of posttranscriptional changes.
31792076	11	81	theme	transcriptional	1588:1602	arg1	differences					1604:1614	no transcriptional differences	1585:1614	no transcriptional differences between the EPT1 overexpression strain and the wild type	1585:1671	In contrast to mutants with decreased PE synthesis, there were no transcriptional differences between the EPT1 overexpression strain and the wild type, indicating that the hypervirulent phenotype is a consequence of posttranscriptional changes.
31792076	9	82	theme	psd1Δ/Δ	1378:1384	arg1	mutant					1394:1399	the psd1Δ/Δ psd2Δ/Δ mutant	1374:1399	the psd1Δ/Δ psd2Δ/Δ mutant	1374:1399	Losses of PSD1 and PSD2, but not EPT1, cause significant increases in transcription of glycosylation genes, which may reflect the substantial cell wall defects in the psd1Δ/Δ psd2Δ/Δ mutant.
31792076	3	83	theme	fungal	615:620	arg1	burden					622:627	decreased kidney fungal burden	598:627	decreased kidney fungal burden in systemically infected mice	598:657	Similarly, an ept1Δ/Δ mutant, which cannot produce PE by the Kennedy pathway, exhibits decreased kidney fungal burden in systemically infected mice.
31792076	9	84	from	increases	1268:1276	arg1	transcription					1281:1293	transcription	1281:1293	transcription of glycosylation genes	1281:1316	Losses of PSD1 and PSD2, but not EPT1, cause significant increases in transcription of glycosylation genes, which may reflect the substantial cell wall defects in the psd1Δ/Δ psd2Δ/Δ mutant.
31792076	12	85	theme	increased	1815:1823	arg1	content					1832:1838	increased chitin content	1815:1838	increased chitin content	1815:1838	It was found that overexpression of EPT1 causes increased chitin content and increased hyphal length.
31792076	11	86	theme	EPT1	1628:1631	arg1	strain					1648:1653	the EPT1 overexpression strain	1624:1653	the EPT1 overexpression strain	1624:1653	In contrast to mutants with decreased PE synthesis, there were no transcriptional differences between the EPT1 overexpression strain and the wild type, indicating that the hypervirulent phenotype is a consequence of posttranscriptional changes.
31792076	4	87	from	phenotype	722:730	arg1	model					740:744	this model	735:744	this model	735:744	Conversely, overexpression of EPT1 results in a hypervirulent phenotype in this model.
31792076	1	88	theme	phosphatidylethanolamine	280:303	arg1	synthesis					250:258	synthesis	250:258	synthesis of the phospholipid phosphatidylethanolamine (PE)	250:308	Candida albicans is a leading cause of systemic bloodstream infections, and synthesis of the phospholipid phosphatidylethanolamine (PE) is required for virulence.
32998076	4	0	theme	detergents	650:659	arg1	change					640:645	gradient change	631:645	gradient change of detergents	631:659	MATERIALS AND METHODS An efficient method for decellularization of Goat urinary bladder (N = 3) was developed by perfusion of gradient change of detergents through ureter.
32998076	5	1	theme	composition	735:745	arg1	retention					681:689	The retention	677:689	The retention of organ architecture, extracellular matrix composition, mechanical properties and removal of cellular components	677:803	The retention of organ architecture, extracellular matrix composition, mechanical properties and removal of cellular components was characterized using histological, cellular and molecular analysis.
32998076	11	2	theme	future	1681:1686	arg1	application					1688:1698	future application	1681:1698	future application	1681:1698	CONCLUSION Biofabricated humanized urinary bladder constructs provides xeno-free allografts for future application in augmenting urinary bladder reconstruction/regeneration with further development.
32998076	2	3	contain	have	296:299	arg1	approaches					257:266	Current therapeutic approaches	237:266	Current therapeutic approaches including enterocystoplasty	237:294	Current therapeutic approaches including enterocystoplasty have several limitations.
32998076	2	3	contain	have	296:299	arg2	limitations					309:319	several limitations	301:319	several limitations	301:319	Current therapeutic approaches including enterocystoplasty have several limitations.
32998076	2	3	contain	have	296:299	arg1	enterocystoplasty					278:294	enterocystoplasty	278:294	enterocystoplasty	278:294	Current therapeutic approaches including enterocystoplasty have several limitations.
32998076	6	4	theme	biological	977:986	arg1	construct					988:996	biological construct	977:996	biological construct of decellularized urinary bladder (DUB) scaffolds to augment the urinary bladder reconstruction/regeneration	977:1105	Further, mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB) were used for preparing biological construct of decellularized urinary bladder (DUB) scaffolds to augment the urinary bladder reconstruction/regeneration.
32998076	7	5	theme	100	1217:1219	arg1	mm					1221:1222	mm	1221:1222	mm	1221:1222	RESULTS The decellularization method adopted in this study generated completely DUB scaffolds within 10 h at 100 mm Hg pressure and constant flow rate of 1 mL/min.
32998076	5	6	theme	architecture	700:711	arg1	retention					681:689	The retention	677:689	The retention of organ architecture, extracellular matrix composition, mechanical properties and removal of cellular components	677:803	The retention of organ architecture, extracellular matrix composition, mechanical properties and removal of cellular components was characterized using histological, cellular and molecular analysis.
32998076	6	7	theme	stem	897:900	arg1	MSCs					909:912	MSCs	909:912	MSCs	909:912	Further, mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB) were used for preparing biological construct of decellularized urinary bladder (DUB) scaffolds to augment the urinary bladder reconstruction/regeneration.
32998076	6	7	theme	stem	897:900	arg1	cells					902:906	mesenchymal stem cells	885:906	mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB)	885:951	Further, mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB) were used for preparing biological construct of decellularized urinary bladder (DUB) scaffolds to augment the urinary bladder reconstruction/regeneration.
32998076	1	8	theme	health	193:198	arg1	conditions					200:209	the most critical health conditions	175:209	the most critical health conditions with no proper treatment	175:234	INTRODUCTION Bladder dysfunction has been considered as one of the most critical health conditions with no proper treatment.
32998076	6	9	from	blood	941:945	arg1	MSCs					909:912	MSCs	909:912	MSCs	909:912	Further, mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB) were used for preparing biological construct of decellularized urinary bladder (DUB) scaffolds to augment the urinary bladder reconstruction/regeneration.
32998076	6	9	from	blood	941:945	arg1	cells					902:906	mesenchymal stem cells	885:906	mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB)	885:951	Further, mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB) were used for preparing biological construct of decellularized urinary bladder (DUB) scaffolds to augment the urinary bladder reconstruction/regeneration.
32998076	5	10	theme	extracellular	714:726	arg1	composition					735:745	extracellular matrix composition	714:745	extracellular matrix composition	714:745	The retention of organ architecture, extracellular matrix composition, mechanical properties and removal of cellular components was characterized using histological, cellular and molecular analysis.
32998076	7	11	theme	Hg	1224:1225	arg1	pressure					1227:1234	100 mm Hg pressure	1217:1234	100 mm Hg pressure	1217:1234	RESULTS The decellularization method adopted in this study generated completely DUB scaffolds within 10 h at 100 mm Hg pressure and constant flow rate of 1 mL/min.
32998076	5	12	theme	components	794:803	arg1	removal					774:780	removal	774:780	removal of cellular components	774:803	The retention of organ architecture, extracellular matrix composition, mechanical properties and removal of cellular components was characterized using histological, cellular and molecular analysis.
32998076	5	12	theme	components	794:803	arg1	composition					735:745	extracellular matrix composition	714:745	extracellular matrix composition	714:745	The retention of organ architecture, extracellular matrix composition, mechanical properties and removal of cellular components was characterized using histological, cellular and molecular analysis.
32998076	5	12	theme	components	794:803	arg1	properties					759:768	mechanical properties	748:768	mechanical properties	748:768	The retention of organ architecture, extracellular matrix composition, mechanical properties and removal of cellular components was characterized using histological, cellular and molecular analysis.
32998076	5	12	theme	components	794:803	arg1	architecture					700:711	organ architecture	694:711	organ architecture	694:711	The retention of organ architecture, extracellular matrix composition, mechanical properties and removal of cellular components was characterized using histological, cellular and molecular analysis.
32998076	1	13	theme	proper	219:224	arg1	treatment					226:234	no proper treatment	216:234	no proper treatment	216:234	INTRODUCTION Bladder dysfunction has been considered as one of the most critical health conditions with no proper treatment.
32998076	8	14	theme	organ	1297:1301	arg1	architecture					1303:1314	organ architecture	1297:1314	organ architecture	1297:1314	The DUB scaffold retains organ architecture, ECM composition, and mechanical strength.
32998076	4	15	theme	efficient	530:538	arg1	method					540:545	An efficient method	527:545	An efficient method for decellularization of Goat urinary bladder	527:591	MATERIALS AND METHODS An efficient method for decellularization of Goat urinary bladder (N = 3) was developed by perfusion of gradient change of detergents through ureter.
32998076	3	16	theme	biological	358:367	arg1	allografts					369:378	cell-laden biological allografts	347:378	cell-laden biological allografts using decellularized Goat urinary bladder scaffolds for organ reconstruction/regeneration	347:468	Hence, biofabrication of cell-laden biological allografts using decellularized Goat urinary bladder scaffolds for organ reconstruction/regeneration was major objective of this study.
32998076	2	17	theme	Current	237:243	arg1	approaches					257:266	Current therapeutic approaches	237:266	Current therapeutic approaches including enterocystoplasty	237:294	Current therapeutic approaches including enterocystoplasty have several limitations.
32998076	2	17	theme	Current	237:243	arg1	enterocystoplasty					278:294	enterocystoplasty	278:294	enterocystoplasty	278:294	Current therapeutic approaches including enterocystoplasty have several limitations.
32998076	6	18	theme	urinary	1016:1022	arg1	DUB					1033:1035	DUB	1033:1035	DUB	1033:1035	Further, mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB) were used for preparing biological construct of decellularized urinary bladder (DUB) scaffolds to augment the urinary bladder reconstruction/regeneration.
32998076	6	18	theme	urinary	1016:1022	arg1	bladder					1024:1030	decellularized urinary bladder	1001:1030	decellularized urinary bladder (DUB) scaffolds	1001:1046	Further, mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB) were used for preparing biological construct of decellularized urinary bladder (DUB) scaffolds to augment the urinary bladder reconstruction/regeneration.
32998076	11	19	theme	urinary	1620:1626	arg1	constructs					1636:1645	Biofabricated humanized urinary bladder constructs	1596:1645	CONCLUSION Biofabricated humanized urinary bladder constructs	1585:1645	CONCLUSION Biofabricated humanized urinary bladder constructs provides xeno-free allografts for future application in augmenting urinary bladder reconstruction/regeneration with further development.
32998076	2	20	theme	several	301:307	arg1	limitations					309:319	several limitations	301:319	several limitations	301:319	Current therapeutic approaches including enterocystoplasty have several limitations.
32998076	3	21	theme	Goat	401:404	arg1	scaffolds					422:430	decellularized Goat urinary bladder scaffolds	386:430	decellularized Goat urinary bladder scaffolds	386:430	Hence, biofabrication of cell-laden biological allografts using decellularized Goat urinary bladder scaffolds for organ reconstruction/regeneration was major objective of this study.
32998076	11	22	theme	Biofabricated	1596:1608	arg1	constructs					1636:1645	Biofabricated humanized urinary bladder constructs	1596:1645	CONCLUSION Biofabricated humanized urinary bladder constructs	1585:1645	CONCLUSION Biofabricated humanized urinary bladder constructs provides xeno-free allografts for future application in augmenting urinary bladder reconstruction/regeneration with further development.
32998076	5	23	theme	molecular	856:864	arg1	analysis					866:873	histological, cellular and molecular analysis	829:873	histological, cellular and molecular analysis	829:873	The retention of organ architecture, extracellular matrix composition, mechanical properties and removal of cellular components was characterized using histological, cellular and molecular analysis.
32998076	9	24	theme	significant	1362:1372	arg1	acid					1401:1404	residual nucleic acid	1384:1404	residual nucleic acid	1384:1404	No significant amount of residual nucleic acid was observed post-decellularization.
32998076	9	24	theme	significant	1362:1372	arg1	amount					1374:1379	No significant amount	1359:1379	No significant amount of residual nucleic acid	1359:1404	No significant amount of residual nucleic acid was observed post-decellularization.
32998076	11	25	theme	xeno-free	1656:1664	arg1	allografts					1666:1675	xeno-free allografts	1656:1675	xeno-free allografts for future application	1656:1698	CONCLUSION Biofabricated humanized urinary bladder constructs provides xeno-free allografts for future application in augmenting urinary bladder reconstruction/regeneration with further development.
32998076	0	26	theme	organ	77:81	arg1	reconstruction/regeneration					83:109	organ reconstruction/regeneration	77:109	organ reconstruction/regeneration	77:109	Biofabrication of cell-laden allografts of goat urinary bladder scaffold for organ reconstruction/regeneration.
32998076	5	27	theme	mechanical	748:757	arg1	properties					759:768	mechanical properties	748:768	mechanical properties	748:768	The retention of organ architecture, extracellular matrix composition, mechanical properties and removal of cellular components was characterized using histological, cellular and molecular analysis.
32998076	6	28	theme	bladder	1071:1077	arg1	reconstruction/regeneration					1079:1105	the urinary bladder reconstruction/regeneration	1059:1105	the urinary bladder reconstruction/regeneration	1059:1105	Further, mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB) were used for preparing biological construct of decellularized urinary bladder (DUB) scaffolds to augment the urinary bladder reconstruction/regeneration.
32998076	4	29	theme	=	596:596	arg1	N					594:594	N = 3	594:598	N = 3	594:598	MATERIALS AND METHODS An efficient method for decellularization of Goat urinary bladder (N = 3) was developed by perfusion of gradient change of detergents through ureter.
32998076	4	29	theme	=	596:596	arg1	MATERIALS					505:513	MATERIALS	505:513	MATERIALS	505:513	MATERIALS AND METHODS An efficient method for decellularization of Goat urinary bladder (N = 3) was developed by perfusion of gradient change of detergents through ureter.
32998076	5	30	theme	histological	829:840	arg1	analysis					866:873	histological, cellular and molecular analysis	829:873	histological, cellular and molecular analysis	829:873	The retention of organ architecture, extracellular matrix composition, mechanical properties and removal of cellular components was characterized using histological, cellular and molecular analysis.
32998076	9	31	theme	nucleic	1393:1399	arg1	acid					1401:1404	residual nucleic acid	1384:1404	residual nucleic acid	1384:1404	No significant amount of residual nucleic acid was observed post-decellularization.
32998076	3	32	theme	bladder	414:420	arg1	scaffolds					422:430	decellularized Goat urinary bladder scaffolds	386:430	decellularized Goat urinary bladder scaffolds	386:430	Hence, biofabrication of cell-laden biological allografts using decellularized Goat urinary bladder scaffolds for organ reconstruction/regeneration was major objective of this study.
32998076	7	33	dep	RESULTS	1108:1114	arg1	generated					1167:1175	generated	1167:1175	generated completely DUB scaffolds within 10 h at 100 mm Hg pressure and constant flow rate of 1 mL/min	1167:1269	RESULTS The decellularization method adopted in this study generated completely DUB scaffolds within 10 h at 100 mm Hg pressure and constant flow rate of 1 mL/min.
32998076	5	34	theme	cellular	843:850	arg1	analysis					866:873	histological, cellular and molecular analysis	829:873	histological, cellular and molecular analysis	829:873	The retention of organ architecture, extracellular matrix composition, mechanical properties and removal of cellular components was characterized using histological, cellular and molecular analysis.
32998076	0	35	theme	goat	43:46	arg1	scaffold					64:71	goat urinary bladder scaffold	43:71	goat urinary bladder scaffold for organ reconstruction/regeneration	43:109	Biofabrication of cell-laden allografts of goat urinary bladder scaffold for organ reconstruction/regeneration.
32998076	3	36	theme	major	474:478	arg1	objective					480:488	major objective	474:488	major objective of this study	474:502	Hence, biofabrication of cell-laden biological allografts using decellularized Goat urinary bladder scaffolds for organ reconstruction/regeneration was major objective of this study.
32998076	4	37	theme	urinary	577:583	arg1	bladder					585:591	Goat urinary bladder	572:591	Goat urinary bladder	572:591	MATERIALS AND METHODS An efficient method for decellularization of Goat urinary bladder (N = 3) was developed by perfusion of gradient change of detergents through ureter.
32998076	10	38	theme	xeno-free	1564:1572	arg1	condition					1574:1582	xeno-free condition	1564:1582	xeno-free condition	1564:1582	Furthermore, MSCs derived from human UCB engrafted and proliferated well on DUB scaffolds in highly aligned manner under xeno-free condition.
32998076	7	39	theme	mL/min	1264:1269	arg1	pressure					1227:1234	100 mm Hg pressure	1217:1234	100 mm Hg pressure	1217:1234	RESULTS The decellularization method adopted in this study generated completely DUB scaffolds within 10 h at 100 mm Hg pressure and constant flow rate of 1 mL/min.
32998076	7	39	theme	mL/min	1264:1269	arg1	rate					1254:1257	constant flow rate	1240:1257	constant flow rate of 1 mL/min	1240:1269	RESULTS The decellularization method adopted in this study generated completely DUB scaffolds within 10 h at 100 mm Hg pressure and constant flow rate of 1 mL/min.
32998076	1	40	theme	INTRODUCTION	112:123	arg1	conditions					200:209	the most critical health conditions	175:209	the most critical health conditions with no proper treatment	175:234	INTRODUCTION Bladder dysfunction has been considered as one of the most critical health conditions with no proper treatment.
32998076	1	40	theme	INTRODUCTION	112:123	arg1	one					168:170	one	168:170	one	168:170	INTRODUCTION Bladder dysfunction has been considered as one of the most critical health conditions with no proper treatment.
32998076	1	40	theme	INTRODUCTION	112:123	arg1	dysfunction					133:143	INTRODUCTION Bladder dysfunction	112:143	INTRODUCTION Bladder dysfunction	112:143	INTRODUCTION Bladder dysfunction has been considered as one of the most critical health conditions with no proper treatment.
32998076	0	41	theme	cell-laden	18:27	arg1	allografts					29:38	cell-laden allografts	18:38	cell-laden allografts of goat urinary bladder scaffold for organ reconstruction/regeneration	18:109	Biofabrication of cell-laden allografts of goat urinary bladder scaffold for organ reconstruction/regeneration.
32998076	3	42	theme	study	498:502	arg1	objective					480:488	major objective	474:488	major objective of this study	474:502	Hence, biofabrication of cell-laden biological allografts using decellularized Goat urinary bladder scaffolds for organ reconstruction/regeneration was major objective of this study.
32998076	4	43	theme	gradient	631:638	arg1	change					640:645	gradient change	631:645	gradient change of detergents	631:659	MATERIALS AND METHODS An efficient method for decellularization of Goat urinary bladder (N = 3) was developed by perfusion of gradient change of detergents through ureter.
32998076	7	44	theme	flow	1249:1252	arg1	rate					1254:1257	constant flow rate	1240:1257	constant flow rate of 1 mL/min	1240:1269	RESULTS The decellularization method adopted in this study generated completely DUB scaffolds within 10 h at 100 mm Hg pressure and constant flow rate of 1 mL/min.
32998076	6	45	used	used	958:961	arg2	MSCs					909:912	MSCs	909:912	MSCs	909:912	Further, mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB) were used for preparing biological construct of decellularized urinary bladder (DUB) scaffolds to augment the urinary bladder reconstruction/regeneration.
32998076	6	45	used	used	958:961	arg2	cells					902:906	mesenchymal stem cells	885:906	mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB)	885:951	Further, mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB) were used for preparing biological construct of decellularized urinary bladder (DUB) scaffolds to augment the urinary bladder reconstruction/regeneration.
32998076	0	46	theme	urinary	48:54	arg1	scaffold					64:71	goat urinary bladder scaffold	43:71	goat urinary bladder scaffold for organ reconstruction/regeneration	43:109	Biofabrication of cell-laden allografts of goat urinary bladder scaffold for organ reconstruction/regeneration.
32998076	6	47	theme	cord	936:939	arg1	UCB					948:950	UCB	948:950	UCB	948:950	Further, mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB) were used for preparing biological construct of decellularized urinary bladder (DUB) scaffolds to augment the urinary bladder reconstruction/regeneration.
32998076	6	47	theme	cord	936:939	arg1	blood					941:945	human umbilical cord blood	920:945	human umbilical cord blood (UCB)	920:951	Further, mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB) were used for preparing biological construct of decellularized urinary bladder (DUB) scaffolds to augment the urinary bladder reconstruction/regeneration.
32998076	11	48	theme	urinary	1714:1720	arg1	reconstruction/regeneration					1730:1756	urinary bladder reconstruction/regeneration	1714:1756	urinary bladder reconstruction/regeneration	1714:1756	CONCLUSION Biofabricated humanized urinary bladder constructs provides xeno-free allografts for future application in augmenting urinary bladder reconstruction/regeneration with further development.
32998076	6	49	theme	human	920:924	arg1	UCB					948:950	UCB	948:950	UCB	948:950	Further, mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB) were used for preparing biological construct of decellularized urinary bladder (DUB) scaffolds to augment the urinary bladder reconstruction/regeneration.
32998076	6	49	theme	human	920:924	arg1	blood					941:945	human umbilical cord blood	920:945	human umbilical cord blood (UCB)	920:951	Further, mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB) were used for preparing biological construct of decellularized urinary bladder (DUB) scaffolds to augment the urinary bladder reconstruction/regeneration.
32998076	6	50	theme	decellularized	1001:1014	arg1	DUB					1033:1035	DUB	1033:1035	DUB	1033:1035	Further, mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB) were used for preparing biological construct of decellularized urinary bladder (DUB) scaffolds to augment the urinary bladder reconstruction/regeneration.
32998076	6	50	theme	decellularized	1001:1014	arg1	bladder					1024:1030	decellularized urinary bladder	1001:1030	decellularized urinary bladder (DUB) scaffolds	1001:1046	Further, mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB) were used for preparing biological construct of decellularized urinary bladder (DUB) scaffolds to augment the urinary bladder reconstruction/regeneration.
32998076	5	51	theme	matrix	728:733	arg1	composition					735:745	extracellular matrix composition	714:745	extracellular matrix composition	714:745	The retention of organ architecture, extracellular matrix composition, mechanical properties and removal of cellular components was characterized using histological, cellular and molecular analysis.
32998076	6	52	theme	mesenchymal	885:895	arg1	MSCs					909:912	MSCs	909:912	MSCs	909:912	Further, mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB) were used for preparing biological construct of decellularized urinary bladder (DUB) scaffolds to augment the urinary bladder reconstruction/regeneration.
32998076	6	52	theme	mesenchymal	885:895	arg1	cells					902:906	mesenchymal stem cells	885:906	mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB)	885:951	Further, mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB) were used for preparing biological construct of decellularized urinary bladder (DUB) scaffolds to augment the urinary bladder reconstruction/regeneration.
32998076	5	53	theme	organ	694:698	arg1	architecture					700:711	organ architecture	694:711	organ architecture	694:711	The retention of organ architecture, extracellular matrix composition, mechanical properties and removal of cellular components was characterized using histological, cellular and molecular analysis.
32998076	1	54	theme	critical	184:191	arg1	conditions					200:209	the most critical health conditions	175:209	the most critical health conditions with no proper treatment	175:234	INTRODUCTION Bladder dysfunction has been considered as one of the most critical health conditions with no proper treatment.
32998076	11	55	theme	bladder	1722:1728	arg1	reconstruction/regeneration					1730:1756	urinary bladder reconstruction/regeneration	1714:1756	urinary bladder reconstruction/regeneration	1714:1756	CONCLUSION Biofabricated humanized urinary bladder constructs provides xeno-free allografts for future application in augmenting urinary bladder reconstruction/regeneration with further development.
32998076	7	56	theme	DUB	1188:1190	arg1	scaffolds					1192:1200	DUB scaffolds	1188:1200	DUB scaffolds	1188:1200	RESULTS The decellularization method adopted in this study generated completely DUB scaffolds within 10 h at 100 mm Hg pressure and constant flow rate of 1 mL/min.
32998076	8	57	theme	ECM	1317:1319	arg1	composition					1321:1331	ECM composition	1317:1331	ECM composition	1317:1331	The DUB scaffold retains organ architecture, ECM composition, and mechanical strength.
32998076	10	58	theme	DUB	1519:1521	arg1	scaffolds					1523:1531	DUB scaffolds	1519:1531	DUB scaffolds	1519:1531	Furthermore, MSCs derived from human UCB engrafted and proliferated well on DUB scaffolds in highly aligned manner under xeno-free condition.
32998076	1	59	theme	conditions	200:209	arg1	conditions					200:209	the most critical health conditions	175:209	the most critical health conditions with no proper treatment	175:234	INTRODUCTION Bladder dysfunction has been considered as one of the most critical health conditions with no proper treatment.
32998076	1	59	theme	conditions	200:209	arg1	one					168:170	one	168:170	one	168:170	INTRODUCTION Bladder dysfunction has been considered as one of the most critical health conditions with no proper treatment.
32998076	1	59	theme	conditions	200:209	arg1	dysfunction					133:143	INTRODUCTION Bladder dysfunction	112:143	INTRODUCTION Bladder dysfunction	112:143	INTRODUCTION Bladder dysfunction has been considered as one of the most critical health conditions with no proper treatment.
32998076	5	60	theme	cellular	785:792	arg1	components					794:803	cellular components	785:803	cellular components	785:803	The retention of organ architecture, extracellular matrix composition, mechanical properties and removal of cellular components was characterized using histological, cellular and molecular analysis.
32998076	8	61	theme	DUB	1276:1278	arg1	scaffold					1280:1287	The DUB scaffold	1272:1287	The DUB scaffold	1272:1287	The DUB scaffold retains organ architecture, ECM composition, and mechanical strength.
32998076	6	62	theme	scaffolds	1038:1046	arg1	construct					988:996	biological construct	977:996	biological construct of decellularized urinary bladder (DUB) scaffolds to augment the urinary bladder reconstruction/regeneration	977:1105	Further, mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB) were used for preparing biological construct of decellularized urinary bladder (DUB) scaffolds to augment the urinary bladder reconstruction/regeneration.
32998076	8	63	theme	mechanical	1338:1347	arg1	strength					1349:1356	mechanical strength	1338:1356	mechanical strength	1338:1356	The DUB scaffold retains organ architecture, ECM composition, and mechanical strength.
32998076	10	64	attach	derived	1461:1467	arg1	UCB					1480:1482	human UCB	1474:1482	human UCB	1474:1482	Furthermore, MSCs derived from human UCB engrafted and proliferated well on DUB scaffolds in highly aligned manner under xeno-free condition.
32998076	10	64	attach	derived	1461:1467	arg2	MSCs					1456:1459	MSCs	1456:1459	MSCs derived from human UCB	1456:1482	Furthermore, MSCs derived from human UCB engrafted and proliferated well on DUB scaffolds in highly aligned manner under xeno-free condition.
32998076	5	65	theme	properties	759:768	arg1	retention					681:689	The retention	677:689	The retention of organ architecture, extracellular matrix composition, mechanical properties and removal of cellular components	677:803	The retention of organ architecture, extracellular matrix composition, mechanical properties and removal of cellular components was characterized using histological, cellular and molecular analysis.
32998076	2	66	theme	therapeutic	245:255	arg1	approaches					257:266	Current therapeutic approaches	237:266	Current therapeutic approaches including enterocystoplasty	237:294	Current therapeutic approaches including enterocystoplasty have several limitations.
32998076	2	66	theme	therapeutic	245:255	arg1	enterocystoplasty					278:294	enterocystoplasty	278:294	enterocystoplasty	278:294	Current therapeutic approaches including enterocystoplasty have several limitations.
32998076	7	67	theme	decellularization	1120:1136	arg1	method					1138:1143	The decellularization method	1116:1143	The decellularization method adopted in this study	1116:1165	RESULTS The decellularization method adopted in this study generated completely DUB scaffolds within 10 h at 100 mm Hg pressure and constant flow rate of 1 mL/min.
32998076	6	68	theme	bladder	1024:1030	arg1	scaffolds					1038:1046	decellularized urinary bladder (DUB) scaffolds	1001:1046	decellularized urinary bladder (DUB) scaffolds	1001:1046	Further, mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB) were used for preparing biological construct of decellularized urinary bladder (DUB) scaffolds to augment the urinary bladder reconstruction/regeneration.
32998076	3	69	theme	allografts	369:378	arg1	biofabrication					329:342	biofabrication	329:342	biofabrication of cell-laden biological allografts using decellularized Goat urinary bladder scaffolds for organ reconstruction/regeneration	329:468	Hence, biofabrication of cell-laden biological allografts using decellularized Goat urinary bladder scaffolds for organ reconstruction/regeneration was major objective of this study.
32998076	11	70	theme	humanized	1610:1618	arg1	constructs					1636:1645	Biofabricated humanized urinary bladder constructs	1596:1645	CONCLUSION Biofabricated humanized urinary bladder constructs	1585:1645	CONCLUSION Biofabricated humanized urinary bladder constructs provides xeno-free allografts for future application in augmenting urinary bladder reconstruction/regeneration with further development.
32998076	5	71	theme	removal	774:780	arg1	retention					681:689	The retention	677:689	The retention of organ architecture, extracellular matrix composition, mechanical properties and removal of cellular components	677:803	The retention of organ architecture, extracellular matrix composition, mechanical properties and removal of cellular components was characterized using histological, cellular and molecular analysis.
32998076	3	72	theme	cell-laden	347:356	arg1	allografts					369:378	cell-laden biological allografts	347:378	cell-laden biological allografts using decellularized Goat urinary bladder scaffolds for organ reconstruction/regeneration	347:468	Hence, biofabrication of cell-laden biological allografts using decellularized Goat urinary bladder scaffolds for organ reconstruction/regeneration was major objective of this study.
32998076	3	73	theme	urinary	406:412	arg1	scaffolds					422:430	decellularized Goat urinary bladder scaffolds	386:430	decellularized Goat urinary bladder scaffolds	386:430	Hence, biofabrication of cell-laden biological allografts using decellularized Goat urinary bladder scaffolds for organ reconstruction/regeneration was major objective of this study.
32998076	3	74	theme	decellularized	386:399	arg1	scaffolds					422:430	decellularized Goat urinary bladder scaffolds	386:430	decellularized Goat urinary bladder scaffolds	386:430	Hence, biofabrication of cell-laden biological allografts using decellularized Goat urinary bladder scaffolds for organ reconstruction/regeneration was major objective of this study.
32998076	10	75	theme	aligned	1543:1549	arg1	manner					1551:1556	highly aligned manner	1536:1556	highly aligned manner	1536:1556	Furthermore, MSCs derived from human UCB engrafted and proliferated well on DUB scaffolds in highly aligned manner under xeno-free condition.
32998076	11	76	dep	CONCLUSION	1585:1594	arg1	constructs					1636:1645	Biofabricated humanized urinary bladder constructs	1596:1645	CONCLUSION Biofabricated humanized urinary bladder constructs	1585:1645	CONCLUSION Biofabricated humanized urinary bladder constructs provides xeno-free allografts for future application in augmenting urinary bladder reconstruction/regeneration with further development.
32998076	11	77	theme	bladder	1628:1634	arg1	constructs					1636:1645	Biofabricated humanized urinary bladder constructs	1596:1645	CONCLUSION Biofabricated humanized urinary bladder constructs	1585:1645	CONCLUSION Biofabricated humanized urinary bladder constructs provides xeno-free allografts for future application in augmenting urinary bladder reconstruction/regeneration with further development.
32998076	9	78	theme	residual	1384:1391	arg1	acid					1401:1404	residual nucleic acid	1384:1404	residual nucleic acid	1384:1404	No significant amount of residual nucleic acid was observed post-decellularization.
32998076	6	79	theme	urinary	1063:1069	arg1	bladder					1071:1077	the urinary bladder	1059:1077	the urinary bladder reconstruction/regeneration	1059:1105	Further, mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB) were used for preparing biological construct of decellularized urinary bladder (DUB) scaffolds to augment the urinary bladder reconstruction/regeneration.
32998076	3	80	theme	organ	436:440	arg1	reconstruction/regeneration					442:468	organ reconstruction/regeneration	436:468	organ reconstruction/regeneration	436:468	Hence, biofabrication of cell-laden biological allografts using decellularized Goat urinary bladder scaffolds for organ reconstruction/regeneration was major objective of this study.
32998076	4	81	theme	Goat	572:575	arg1	bladder					585:591	Goat urinary bladder	572:591	Goat urinary bladder	572:591	MATERIALS AND METHODS An efficient method for decellularization of Goat urinary bladder (N = 3) was developed by perfusion of gradient change of detergents through ureter.
32998076	9	82	theme	acid	1401:1404	arg1	acid					1401:1404	residual nucleic acid	1384:1404	residual nucleic acid	1384:1404	No significant amount of residual nucleic acid was observed post-decellularization.
32998076	9	82	theme	acid	1401:1404	arg1	amount					1374:1379	No significant amount	1359:1379	No significant amount of residual nucleic acid	1359:1404	No significant amount of residual nucleic acid was observed post-decellularization.
32998076	4	83	theme	bladder	585:591	arg1	decellularization					551:567	decellularization	551:567	decellularization of Goat urinary bladder	551:591	MATERIALS AND METHODS An efficient method for decellularization of Goat urinary bladder (N = 3) was developed by perfusion of gradient change of detergents through ureter.
32998076	0	84	theme	allografts	29:38	arg1	Biofabrication					0:13	Biofabrication	0:13	Biofabrication of cell-laden allografts of goat urinary bladder scaffold for organ reconstruction/regeneration.	0:110	Biofabrication of cell-laden allografts of goat urinary bladder scaffold for organ reconstruction/regeneration.
32998076	7	85	theme	mm	1221:1222	arg1	pressure					1227:1234	100 mm Hg pressure	1217:1234	100 mm Hg pressure	1217:1234	RESULTS The decellularization method adopted in this study generated completely DUB scaffolds within 10 h at 100 mm Hg pressure and constant flow rate of 1 mL/min.
32998076	1	86	theme	Bladder	125:131	arg1	conditions					200:209	the most critical health conditions	175:209	the most critical health conditions with no proper treatment	175:234	INTRODUCTION Bladder dysfunction has been considered as one of the most critical health conditions with no proper treatment.
32998076	1	86	theme	Bladder	125:131	arg1	one					168:170	one	168:170	one	168:170	INTRODUCTION Bladder dysfunction has been considered as one of the most critical health conditions with no proper treatment.
32998076	1	86	theme	Bladder	125:131	arg1	dysfunction					133:143	INTRODUCTION Bladder dysfunction	112:143	INTRODUCTION Bladder dysfunction	112:143	INTRODUCTION Bladder dysfunction has been considered as one of the most critical health conditions with no proper treatment.
32998076	4	87	theme	change	640:645	arg1	perfusion					618:626	perfusion	618:626	perfusion of gradient change of detergents through ureter	618:674	MATERIALS AND METHODS An efficient method for decellularization of Goat urinary bladder (N = 3) was developed by perfusion of gradient change of detergents through ureter.
32998076	7	88	theme	constant	1240:1247	arg1	rate					1254:1257	constant flow rate	1240:1257	constant flow rate of 1 mL/min	1240:1269	RESULTS The decellularization method adopted in this study generated completely DUB scaffolds within 10 h at 100 mm Hg pressure and constant flow rate of 1 mL/min.
32998076	11	89	theme	further	1763:1769	arg1	development					1771:1781	further development	1763:1781	further development	1763:1781	CONCLUSION Biofabricated humanized urinary bladder constructs provides xeno-free allografts for future application in augmenting urinary bladder reconstruction/regeneration with further development.
32998076	0	90	theme	bladder	56:62	arg1	scaffold					64:71	goat urinary bladder scaffold	43:71	goat urinary bladder scaffold for organ reconstruction/regeneration	43:109	Biofabrication of cell-laden allografts of goat urinary bladder scaffold for organ reconstruction/regeneration.
32998076	1	91	with	conditions	200:209	arg1	treatment					226:234	no proper treatment	216:234	no proper treatment	216:234	INTRODUCTION Bladder dysfunction has been considered as one of the most critical health conditions with no proper treatment.
32998076	0	92	theme	scaffold	64:71	arg1	allografts					29:38	cell-laden allografts	18:38	cell-laden allografts of goat urinary bladder scaffold for organ reconstruction/regeneration	18:109	Biofabrication of cell-laden allografts of goat urinary bladder scaffold for organ reconstruction/regeneration.
32998076	6	93	theme	umbilical	926:934	arg1	UCB					948:950	UCB	948:950	UCB	948:950	Further, mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB) were used for preparing biological construct of decellularized urinary bladder (DUB) scaffolds to augment the urinary bladder reconstruction/regeneration.
32998076	6	93	theme	umbilical	926:934	arg1	blood					941:945	human umbilical cord blood	920:945	human umbilical cord blood (UCB)	920:951	Further, mesenchymal stem cells (MSCs) from human umbilical cord blood (UCB) were used for preparing biological construct of decellularized urinary bladder (DUB) scaffolds to augment the urinary bladder reconstruction/regeneration.
32998076	10	94	theme	human	1474:1478	arg1	UCB					1480:1482	human UCB	1474:1482	human UCB	1474:1482	Furthermore, MSCs derived from human UCB engrafted and proliferated well on DUB scaffolds in highly aligned manner under xeno-free condition.
32998076	4	95	dep	MATERIALS	505:513	arg1	method					540:545	An efficient method	527:545	An efficient method for decellularization of Goat urinary bladder	527:591	MATERIALS AND METHODS An efficient method for decellularization of Goat urinary bladder (N = 3) was developed by perfusion of gradient change of detergents through ureter.
33093521	10	0	theme	periodontitis	1620:1632	arg1	treatment					1634:1642	periodontitis treatment	1620:1642	periodontitis treatment	1620:1642	Thus, it can be used as an excellent candidate for periodontitis treatment.
33093521	7	1	from	increase	1063:1070	arg1	formation					1134:1142	biofilm formation	1126:1142	biofilm formation	1126:1142	Moreover, a significant increase in malondialdehyde (MDA) production and a decrease in biofilm formation and metabolic activity of the bacteria was observed in the presence of hydrogel.
33093521	7	1	from	increase	1063:1070	arg1	activity					1158:1165	metabolic activity	1148:1165	metabolic activity	1148:1165	Moreover, a significant increase in malondialdehyde (MDA) production and a decrease in biofilm formation and metabolic activity of the bacteria was observed in the presence of hydrogel.
33093521	7	1	from	increase	1063:1070	arg1	production					1097:1106	malondialdehyde (MDA) production	1075:1106	malondialdehyde (MDA) production	1075:1106	Moreover, a significant increase in malondialdehyde (MDA) production and a decrease in biofilm formation and metabolic activity of the bacteria was observed in the presence of hydrogel.
33093521	9	2	theme	antibacterial	1486:1498	arg1	properties					1522:1531	antibacterial and anti-inflammatory properties	1486:1531	antibacterial and anti-inflammatory properties	1486:1531	In conclusion, the hydrogels were successfully developed and proven to have antibacterial and anti-inflammatory properties for the treatment of periodontitis.
33093521	0	3	theme	composite	96:104	arg1	hydrogels					106:114	cellulose nanofiber and κ-carrageenan oligosaccharide composite hydrogels	42:114	hydrogels	106:114	Therapeutic effects of antibiotics loaded cellulose nanofiber and κ-carrageenan oligosaccharide composite hydrogels for periodontitis treatment.
33093521	7	4	theme	hydrogel	1215:1222	arg1	presence					1203:1210	the presence	1199:1210	the presence of hydrogel	1199:1222	Moreover, a significant increase in malondialdehyde (MDA) production and a decrease in biofilm formation and metabolic activity of the bacteria was observed in the presence of hydrogel.
33093521	9	5	contain	have	1481:1484	arg2	properties					1522:1531	antibacterial and anti-inflammatory properties	1486:1531	antibacterial and anti-inflammatory properties	1486:1531	In conclusion, the hydrogels were successfully developed and proven to have antibacterial and anti-inflammatory properties for the treatment of periodontitis.
33093521	9	5	contain	have	1481:1484	arg1	hydrogels					1429:1437	the hydrogels	1425:1437	the hydrogels	1425:1437	In conclusion, the hydrogels were successfully developed and proven to have antibacterial and anti-inflammatory properties for the treatment of periodontitis.
33093521	0	6	theme	periodontitis	120:132	arg1	treatment					134:142	periodontitis treatment	120:142	periodontitis treatment	120:142	Therapeutic effects of antibiotics loaded cellulose nanofiber and κ-carrageenan oligosaccharide composite hydrogels for periodontitis treatment.
33093521	6	7	theme	antibacterial	877:889	arg1	activity					891:898	strong antibacterial activity	870:898	strong antibacterial activity against periodontal pathogens such as Streptococcus mutans, Porphyromonas gingivalis, Fusobacterium nucleatum, and Pseudomonas aeruginosa	870:1036	This material has strong antibacterial activity against periodontal pathogens such as Streptococcus mutans, Porphyromonas gingivalis, Fusobacterium nucleatum, and Pseudomonas aeruginosa.
33093521	9	8	theme	anti-inflammatory	1504:1520	arg1	properties					1522:1531	antibacterial and anti-inflammatory properties	1486:1531	antibacterial and anti-inflammatory properties	1486:1531	In conclusion, the hydrogels were successfully developed and proven to have antibacterial and anti-inflammatory properties for the treatment of periodontitis.
33093521	6	9	theme	strong	870:875	arg1	activity					891:898	strong antibacterial activity	870:898	strong antibacterial activity against periodontal pathogens such as Streptococcus mutans, Porphyromonas gingivalis, Fusobacterium nucleatum, and Pseudomonas aeruginosa	870:1036	This material has strong antibacterial activity against periodontal pathogens such as Streptococcus mutans, Porphyromonas gingivalis, Fusobacterium nucleatum, and Pseudomonas aeruginosa.
33093521	9	10	theme	periodontitis	1554:1566	arg1	treatment					1541:1549	the treatment	1537:1549	the treatment of periodontitis	1537:1566	In conclusion, the hydrogels were successfully developed and proven to have antibacterial and anti-inflammatory properties for the treatment of periodontitis.
33093521	4	11	theme	proposed	635:642	arg1	material					644:651	The proposed material	631:651	The proposed material	631:651	The proposed material has high thermal stability, controlled release, and water absorption capacity.
33093521	5	12	theme	antibacterial	787:799	arg1	properties					823:832	the in vitro antibacterial and anti-inflammatory properties	774:832	the in vitro antibacterial and anti-inflammatory properties of the hydrogels	774:849	This study was proceeded by investigating the in vitro antibacterial and anti-inflammatory properties of the hydrogels.
33093521	3	13	theme	therapeutic	526:536	arg1	surfactin					478:486	surfactin	478:486	surfactin	478:486	Two antimicrobial agents such as surfactin and Herbmedotcin were selected as the therapeutic agents and the hydrogels were formulated based on the increasing concentration of surfactin.
33093521	3	13	theme	therapeutic	526:536	arg1	Herbmedotcin					492:503	Herbmedotcin	492:503	Herbmedotcin	492:503	Two antimicrobial agents such as surfactin and Herbmedotcin were selected as the therapeutic agents and the hydrogels were formulated based on the increasing concentration of surfactin.
33093521	3	13	theme	therapeutic	526:536	arg1	agents					463:468	Two antimicrobial agents	445:468	Two antimicrobial agents such as surfactin and Herbmedotcin	445:503	Two antimicrobial agents such as surfactin and Herbmedotcin were selected as the therapeutic agents and the hydrogels were formulated based on the increasing concentration of surfactin.
33093521	3	13	theme	therapeutic	526:536	arg1	agents					538:543	the therapeutic agents	522:543	the therapeutic agents	522:543	Two antimicrobial agents such as surfactin and Herbmedotcin were selected as the therapeutic agents and the hydrogels were formulated based on the increasing concentration of surfactin.
33093521	7	14	theme	significant	1051:1061	arg1	increase					1063:1070	a significant increase	1049:1070	a significant increase in malondialdehyde (MDA) production	1049:1106	Moreover, a significant increase in malondialdehyde (MDA) production and a decrease in biofilm formation and metabolic activity of the bacteria was observed in the presence of hydrogel.
33093521	4	15	theme	water	705:709	arg1	capacity					722:729	water absorption capacity	705:729	water absorption capacity	705:729	The proposed material has high thermal stability, controlled release, and water absorption capacity.
33093521	8	16	theme	reactive	1249:1256	arg1	ROS					1274:1276	ROS	1274:1276	ROS	1274:1276	Besides, it reduced the reactive oxygen species (ROS) generation, transcription factor, and cytokines production in human gingival fibroblast cells (HGF) under inflammatory conditions.
33093521	8	16	theme	reactive	1249:1256	arg1	species					1265:1271	reactive oxygen species	1249:1271	the reactive oxygen species (ROS) generation	1245:1288	Besides, it reduced the reactive oxygen species (ROS) generation, transcription factor, and cytokines production in human gingival fibroblast cells (HGF) under inflammatory conditions.
33093521	5	17	theme	hydrogels	841:849	arg1	properties					823:832	the in vitro antibacterial and anti-inflammatory properties	774:832	the in vitro antibacterial and anti-inflammatory properties of the hydrogels	774:849	This study was proceeded by investigating the in vitro antibacterial and anti-inflammatory properties of the hydrogels.
33093521	8	18	theme	species	1265:1271	arg1	generation					1279:1288	the reactive oxygen species (ROS) generation	1245:1288	the reactive oxygen species (ROS) generation	1245:1288	Besides, it reduced the reactive oxygen species (ROS) generation, transcription factor, and cytokines production in human gingival fibroblast cells (HGF) under inflammatory conditions.
33093521	8	18	theme	species	1265:1271	arg1	factor					1305:1310	transcription factor	1291:1310	transcription factor	1291:1310	Besides, it reduced the reactive oxygen species (ROS) generation, transcription factor, and cytokines production in human gingival fibroblast cells (HGF) under inflammatory conditions.
33093521	8	19	theme	gingival	1347:1354	arg1	HGF					1374:1376	HGF	1374:1376	HGF	1374:1376	Besides, it reduced the reactive oxygen species (ROS) generation, transcription factor, and cytokines production in human gingival fibroblast cells (HGF) under inflammatory conditions.
33093521	8	19	theme	gingival	1347:1354	arg1	cells					1367:1371	human gingival fibroblast cells	1341:1371	human gingival fibroblast cells (HGF)	1341:1377	Besides, it reduced the reactive oxygen species (ROS) generation, transcription factor, and cytokines production in human gingival fibroblast cells (HGF) under inflammatory conditions.
33093521	8	20	theme	oxygen	1258:1263	arg1	ROS					1274:1276	ROS	1274:1276	ROS	1274:1276	Besides, it reduced the reactive oxygen species (ROS) generation, transcription factor, and cytokines production in human gingival fibroblast cells (HGF) under inflammatory conditions.
33093521	8	20	theme	oxygen	1258:1263	arg1	species					1265:1271	reactive oxygen species	1249:1271	the reactive oxygen species (ROS) generation	1245:1288	Besides, it reduced the reactive oxygen species (ROS) generation, transcription factor, and cytokines production in human gingival fibroblast cells (HGF) under inflammatory conditions.
33093521	6	21	theme	Porphyromonas	960:972	arg1	gingivalis					974:983	Porphyromonas gingivalis	960:983	Porphyromonas gingivalis	960:983	This material has strong antibacterial activity against periodontal pathogens such as Streptococcus mutans, Porphyromonas gingivalis, Fusobacterium nucleatum, and Pseudomonas aeruginosa.
33093521	7	22	theme	bacteria	1174:1181	arg1	formation					1134:1142	biofilm formation	1126:1142	biofilm formation	1126:1142	Moreover, a significant increase in malondialdehyde (MDA) production and a decrease in biofilm formation and metabolic activity of the bacteria was observed in the presence of hydrogel.
33093521	7	22	theme	bacteria	1174:1181	arg1	activity					1158:1165	metabolic activity	1148:1165	metabolic activity	1148:1165	Moreover, a significant increase in malondialdehyde (MDA) production and a decrease in biofilm formation and metabolic activity of the bacteria was observed in the presence of hydrogel.
33093521	5	23	theme	anti-inflammatory	805:821	arg1	properties					823:832	the in vitro antibacterial and anti-inflammatory properties	774:832	the in vitro antibacterial and anti-inflammatory properties of the hydrogels	774:849	This study was proceeded by investigating the in vitro antibacterial and anti-inflammatory properties of the hydrogels.
33093521	1	24	theme	periodontal	207:217	arg1	formation					226:234	the periodontal pocket formation	203:234	the periodontal pocket formation	203:234	Periodontitis is an inflammatory disease that can lead to the periodontal pocket formation and tooth loss.
33093521	0	25	theme	Therapeutic	0:10	arg1	effects					12:18	Therapeutic effects	0:18	Therapeutic effects of antibiotics	0:33	Therapeutic effects of antibiotics loaded cellulose nanofiber and κ-carrageenan oligosaccharide composite hydrogels for periodontitis treatment.
33093521	3	26	theme	surfactin	620:628	arg1	concentration					603:615	the increasing concentration	588:615	the increasing concentration of surfactin	588:628	Two antimicrobial agents such as surfactin and Herbmedotcin were selected as the therapeutic agents and the hydrogels were formulated based on the increasing concentration of surfactin.
33093521	1	27	theme	pocket	219:224	arg1	formation					226:234	the periodontal pocket formation	203:234	the periodontal pocket formation	203:234	Periodontitis is an inflammatory disease that can lead to the periodontal pocket formation and tooth loss.
33093521	0	28	theme	antibiotics	23:33	arg1	effects					12:18	Therapeutic effects	0:18	Therapeutic effects of antibiotics	0:33	Therapeutic effects of antibiotics loaded cellulose nanofiber and κ-carrageenan oligosaccharide composite hydrogels for periodontitis treatment.
33093521	3	29	theme	increasing	592:601	arg1	concentration					603:615	the increasing concentration	588:615	the increasing concentration of surfactin	588:628	Two antimicrobial agents such as surfactin and Herbmedotcin were selected as the therapeutic agents and the hydrogels were formulated based on the increasing concentration of surfactin.
33093521	4	30	theme	controlled	681:690	arg1	release					692:698	controlled release	681:698	controlled release	681:698	The proposed material has high thermal stability, controlled release, and water absorption capacity.
33093521	2	31	theme	cellulose	328:336	arg1	nanofibers					338:347	cellulose nanofibers	328:347	cellulose nanofibers (CNF)	328:353	This study was aimed to develop antimicrobials loaded hydrogels composed of cellulose nanofibers (CNF) and κ-carrageenan oligosaccharides (CO) nanoparticles for the treatment of periodontitis.
33093521	2	31	theme	cellulose	328:336	arg1	CNF					350:352	CNF	350:352	CNF	350:352	This study was aimed to develop antimicrobials loaded hydrogels composed of cellulose nanofibers (CNF) and κ-carrageenan oligosaccharides (CO) nanoparticles for the treatment of periodontitis.
33093521	0	32	theme	cellulose	42:50	arg1	nanofiber					52:60	cellulose nanofiber and κ-carrageenan oligosaccharide composite hydrogels	42:114	nanofiber	52:60	Therapeutic effects of antibiotics loaded cellulose nanofiber and κ-carrageenan oligosaccharide composite hydrogels for periodontitis treatment.
33093521	7	33	theme	metabolic	1148:1156	arg1	activity					1158:1165	metabolic activity	1148:1165	metabolic activity	1148:1165	Moreover, a significant increase in malondialdehyde (MDA) production and a decrease in biofilm formation and metabolic activity of the bacteria was observed in the presence of hydrogel.
33093521	8	34	theme	fibroblast	1356:1365	arg1	HGF					1374:1376	HGF	1374:1376	HGF	1374:1376	Besides, it reduced the reactive oxygen species (ROS) generation, transcription factor, and cytokines production in human gingival fibroblast cells (HGF) under inflammatory conditions.
33093521	8	34	theme	fibroblast	1356:1365	arg1	cells					1367:1371	human gingival fibroblast cells	1341:1371	human gingival fibroblast cells (HGF)	1341:1377	Besides, it reduced the reactive oxygen species (ROS) generation, transcription factor, and cytokines production in human gingival fibroblast cells (HGF) under inflammatory conditions.
33093521	4	35	theme	absorption	711:720	arg1	capacity					722:729	water absorption capacity	705:729	water absorption capacity	705:729	The proposed material has high thermal stability, controlled release, and water absorption capacity.
33093521	1	36	theme	tooth	240:244	arg1	loss					246:249	tooth loss	240:249	tooth loss	240:249	Periodontitis is an inflammatory disease that can lead to the periodontal pocket formation and tooth loss.
33093521	3	37	theme	antimicrobial	449:461	arg1	surfactin					478:486	surfactin	478:486	surfactin	478:486	Two antimicrobial agents such as surfactin and Herbmedotcin were selected as the therapeutic agents and the hydrogels were formulated based on the increasing concentration of surfactin.
33093521	3	37	theme	antimicrobial	449:461	arg1	Herbmedotcin					492:503	Herbmedotcin	492:503	Herbmedotcin	492:503	Two antimicrobial agents such as surfactin and Herbmedotcin were selected as the therapeutic agents and the hydrogels were formulated based on the increasing concentration of surfactin.
33093521	3	37	theme	antimicrobial	449:461	arg1	agents					463:468	Two antimicrobial agents	445:468	Two antimicrobial agents such as surfactin and Herbmedotcin	445:503	Two antimicrobial agents such as surfactin and Herbmedotcin were selected as the therapeutic agents and the hydrogels were formulated based on the increasing concentration of surfactin.
33093521	3	37	theme	antimicrobial	449:461	arg1	agents					538:543	the therapeutic agents	522:543	the therapeutic agents	522:543	Two antimicrobial agents such as surfactin and Herbmedotcin were selected as the therapeutic agents and the hydrogels were formulated based on the increasing concentration of surfactin.
33093521	6	38	dep	Streptococcus	938:950	arg1	mutans					952:957	Streptococcus mutans	938:957	Streptococcus mutans	938:957	This material has strong antibacterial activity against periodontal pathogens such as Streptococcus mutans, Porphyromonas gingivalis, Fusobacterium nucleatum, and Pseudomonas aeruginosa.
33093521	8	39	dep	generation	1279:1288	arg1	production					1327:1336	production	1327:1336	production	1327:1336	Besides, it reduced the reactive oxygen species (ROS) generation, transcription factor, and cytokines production in human gingival fibroblast cells (HGF) under inflammatory conditions.
33093521	2	40	theme	periodontitis	430:442	arg1	treatment					417:425	the treatment	413:425	the treatment of periodontitis	413:442	This study was aimed to develop antimicrobials loaded hydrogels composed of cellulose nanofibers (CNF) and κ-carrageenan oligosaccharides (CO) nanoparticles for the treatment of periodontitis.
33093521	5	41	theme	in	778:779	arg1	properties					823:832	the in vitro antibacterial and anti-inflammatory properties	774:832	the in vitro antibacterial and anti-inflammatory properties of the hydrogels	774:849	This study was proceeded by investigating the in vitro antibacterial and anti-inflammatory properties of the hydrogels.
33093521	2	42	theme	oligosaccharides	373:388	arg1	nanoparticles					395:407	cellulose nanofibers (CNF) and κ-carrageenan oligosaccharides (CO) nanoparticles	328:407	cellulose nanofibers (CNF) and κ-carrageenan oligosaccharides (CO) nanoparticles for the treatment of periodontitis	328:442	This study was aimed to develop antimicrobials loaded hydrogels composed of cellulose nanofibers (CNF) and κ-carrageenan oligosaccharides (CO) nanoparticles for the treatment of periodontitis.
33093521	8	43	theme	transcription	1291:1303	arg1	generation					1279:1288	the reactive oxygen species (ROS) generation	1245:1288	the reactive oxygen species (ROS) generation	1245:1288	Besides, it reduced the reactive oxygen species (ROS) generation, transcription factor, and cytokines production in human gingival fibroblast cells (HGF) under inflammatory conditions.
33093521	8	43	theme	transcription	1291:1303	arg1	factor					1305:1310	transcription factor	1291:1310	transcription factor	1291:1310	Besides, it reduced the reactive oxygen species (ROS) generation, transcription factor, and cytokines production in human gingival fibroblast cells (HGF) under inflammatory conditions.
33093521	10	44	theme	excellent	1596:1604	arg1	it					1575:1576	it	1575:1576	it	1575:1576	Thus, it can be used as an excellent candidate for periodontitis treatment.
33093521	10	44	theme	excellent	1596:1604	arg1	candidate					1606:1614	an excellent candidate	1593:1614	an excellent candidate for periodontitis treatment	1593:1642	Thus, it can be used as an excellent candidate for periodontitis treatment.
33093521	2	45	theme	κ-carrageenan	359:371	arg1	CO					391:392	CO	391:392	CO	391:392	This study was aimed to develop antimicrobials loaded hydrogels composed of cellulose nanofibers (CNF) and κ-carrageenan oligosaccharides (CO) nanoparticles for the treatment of periodontitis.
33093521	2	45	theme	κ-carrageenan	359:371	arg1	oligosaccharides					373:388	κ-carrageenan oligosaccharides	359:388	κ-carrageenan oligosaccharides (CO)	359:393	This study was aimed to develop antimicrobials loaded hydrogels composed of cellulose nanofibers (CNF) and κ-carrageenan oligosaccharides (CO) nanoparticles for the treatment of periodontitis.
33093521	5	46	dep	in	778:779	arg1	vitro					781:785	vitro	781:785	vitro	781:785	This study was proceeded by investigating the in vitro antibacterial and anti-inflammatory properties of the hydrogels.
33093521	6	47	contain	has	866:868	arg2	activity					891:898	strong antibacterial activity	870:898	strong antibacterial activity against periodontal pathogens such as Streptococcus mutans, Porphyromonas gingivalis, Fusobacterium nucleatum, and Pseudomonas aeruginosa	870:1036	This material has strong antibacterial activity against periodontal pathogens such as Streptococcus mutans, Porphyromonas gingivalis, Fusobacterium nucleatum, and Pseudomonas aeruginosa.
33093521	6	47	contain	has	866:868	arg1	material					857:864	This material	852:864	This material	852:864	This material has strong antibacterial activity against periodontal pathogens such as Streptococcus mutans, Porphyromonas gingivalis, Fusobacterium nucleatum, and Pseudomonas aeruginosa.
33093521	2	48	theme	nanofibers	338:347	arg1	nanoparticles					395:407	cellulose nanofibers (CNF) and κ-carrageenan oligosaccharides (CO) nanoparticles	328:407	cellulose nanofibers (CNF) and κ-carrageenan oligosaccharides (CO) nanoparticles for the treatment of periodontitis	328:442	This study was aimed to develop antimicrobials loaded hydrogels composed of cellulose nanofibers (CNF) and κ-carrageenan oligosaccharides (CO) nanoparticles for the treatment of periodontitis.
33093521	4	49	theme	high	657:660	arg1	stability					670:678	high thermal stability	657:678	high thermal stability	657:678	The proposed material has high thermal stability, controlled release, and water absorption capacity.
33093521	7	50	theme	biofilm	1126:1132	arg1	formation					1134:1142	biofilm formation	1126:1142	biofilm formation	1126:1142	Moreover, a significant increase in malondialdehyde (MDA) production and a decrease in biofilm formation and metabolic activity of the bacteria was observed in the presence of hydrogel.
33093521	6	51	theme	periodontal	908:918	arg1	pathogens					920:928	periodontal pathogens	908:928	periodontal pathogens such as Streptococcus mutans, Porphyromonas gingivalis, Fusobacterium nucleatum, and Pseudomonas aeruginosa	908:1036	This material has strong antibacterial activity against periodontal pathogens such as Streptococcus mutans, Porphyromonas gingivalis, Fusobacterium nucleatum, and Pseudomonas aeruginosa.
33093521	6	51	theme	periodontal	908:918	arg1	Streptococcus					938:950	Streptococcus	938:950	Streptococcus	938:950	This material has strong antibacterial activity against periodontal pathogens such as Streptococcus mutans, Porphyromonas gingivalis, Fusobacterium nucleatum, and Pseudomonas aeruginosa.
33093521	6	51	theme	periodontal	908:918	arg1	aeruginosa					1027:1036	Pseudomonas aeruginosa	1015:1036	Pseudomonas aeruginosa	1015:1036	This material has strong antibacterial activity against periodontal pathogens such as Streptococcus mutans, Porphyromonas gingivalis, Fusobacterium nucleatum, and Pseudomonas aeruginosa.
33093521	6	51	theme	periodontal	908:918	arg1	Fusobacterium					986:998	Fusobacterium	986:998	Fusobacterium	986:998	This material has strong antibacterial activity against periodontal pathogens such as Streptococcus mutans, Porphyromonas gingivalis, Fusobacterium nucleatum, and Pseudomonas aeruginosa.
33093521	6	51	theme	periodontal	908:918	arg1	gingivalis					974:983	Porphyromonas gingivalis	960:983	Porphyromonas gingivalis	960:983	This material has strong antibacterial activity against periodontal pathogens such as Streptococcus mutans, Porphyromonas gingivalis, Fusobacterium nucleatum, and Pseudomonas aeruginosa.
33093521	1	52	theme	inflammatory	165:176	arg1	Periodontitis					145:157	Periodontitis	145:157	Periodontitis	145:157	Periodontitis is an inflammatory disease that can lead to the periodontal pocket formation and tooth loss.
33093521	1	52	theme	inflammatory	165:176	arg1	disease					178:184	an inflammatory disease	162:184	an inflammatory disease that can lead to the periodontal pocket formation and tooth loss	162:249	Periodontitis is an inflammatory disease that can lead to the periodontal pocket formation and tooth loss.
33093521	0	53	theme	oligosaccharide	80:94	arg1	hydrogels					106:114	cellulose nanofiber and κ-carrageenan oligosaccharide composite hydrogels	42:114	hydrogels	106:114	Therapeutic effects of antibiotics loaded cellulose nanofiber and κ-carrageenan oligosaccharide composite hydrogels for periodontitis treatment.
33093521	7	54	from	decrease	1114:1121	arg1	formation					1134:1142	biofilm formation	1126:1142	biofilm formation	1126:1142	Moreover, a significant increase in malondialdehyde (MDA) production and a decrease in biofilm formation and metabolic activity of the bacteria was observed in the presence of hydrogel.
33093521	7	54	from	decrease	1114:1121	arg1	activity					1158:1165	metabolic activity	1148:1165	metabolic activity	1148:1165	Moreover, a significant increase in malondialdehyde (MDA) production and a decrease in biofilm formation and metabolic activity of the bacteria was observed in the presence of hydrogel.
33093521	7	54	from	decrease	1114:1121	arg1	production					1097:1106	malondialdehyde (MDA) production	1075:1106	malondialdehyde (MDA) production	1075:1106	Moreover, a significant increase in malondialdehyde (MDA) production and a decrease in biofilm formation and metabolic activity of the bacteria was observed in the presence of hydrogel.
33093521	4	55	contain	has	653:655	arg2	stability					670:678	high thermal stability	657:678	high thermal stability	657:678	The proposed material has high thermal stability, controlled release, and water absorption capacity.
33093521	4	55	contain	has	653:655	arg2	capacity					722:729	water absorption capacity	705:729	water absorption capacity	705:729	The proposed material has high thermal stability, controlled release, and water absorption capacity.
33093521	4	55	contain	has	653:655	arg1	material					644:651	The proposed material	631:651	The proposed material	631:651	The proposed material has high thermal stability, controlled release, and water absorption capacity.
33093521	4	55	contain	has	653:655	arg2	release					692:698	controlled release	681:698	controlled release	681:698	The proposed material has high thermal stability, controlled release, and water absorption capacity.
33093521	8	56	theme	human	1341:1345	arg1	HGF					1374:1376	HGF	1374:1376	HGF	1374:1376	Besides, it reduced the reactive oxygen species (ROS) generation, transcription factor, and cytokines production in human gingival fibroblast cells (HGF) under inflammatory conditions.
33093521	8	56	theme	human	1341:1345	arg1	cells					1367:1371	human gingival fibroblast cells	1341:1371	human gingival fibroblast cells (HGF)	1341:1377	Besides, it reduced the reactive oxygen species (ROS) generation, transcription factor, and cytokines production in human gingival fibroblast cells (HGF) under inflammatory conditions.
33093521	8	57	theme	inflammatory	1385:1396	arg1	conditions					1398:1407	inflammatory conditions	1385:1407	inflammatory conditions	1385:1407	Besides, it reduced the reactive oxygen species (ROS) generation, transcription factor, and cytokines production in human gingival fibroblast cells (HGF) under inflammatory conditions.
33093521	7	58	located	observed	1187:1194	arg2	decrease					1114:1121	a decrease	1112:1121	a decrease in biofilm formation and metabolic activity of the bacteria	1112:1181	Moreover, a significant increase in malondialdehyde (MDA) production and a decrease in biofilm formation and metabolic activity of the bacteria was observed in the presence of hydrogel.
33093521	7	58	located	observed	1187:1194	arg1	presence					1203:1210	the presence	1199:1210	the presence of hydrogel	1199:1222	Moreover, a significant increase in malondialdehyde (MDA) production and a decrease in biofilm formation and metabolic activity of the bacteria was observed in the presence of hydrogel.
33093521	7	58	located	observed	1187:1194	arg2	increase					1063:1070	a significant increase	1049:1070	a significant increase in malondialdehyde (MDA) production	1049:1106	Moreover, a significant increase in malondialdehyde (MDA) production and a decrease in biofilm formation and metabolic activity of the bacteria was observed in the presence of hydrogel.
33093521	6	59	dep	Fusobacterium	986:998	arg1	nucleatum					1000:1008	Fusobacterium nucleatum	986:1008	Fusobacterium nucleatum	986:1008	This material has strong antibacterial activity against periodontal pathogens such as Streptococcus mutans, Porphyromonas gingivalis, Fusobacterium nucleatum, and Pseudomonas aeruginosa.
33093521	0	60	theme	κ-carrageenan	66:78	arg1	hydrogels					106:114	cellulose nanofiber and κ-carrageenan oligosaccharide composite hydrogels	42:114	hydrogels	106:114	Therapeutic effects of antibiotics loaded cellulose nanofiber and κ-carrageenan oligosaccharide composite hydrogels for periodontitis treatment.
33093521	7	61	theme	malondialdehyde	1075:1089	arg1	production					1097:1106	malondialdehyde (MDA) production	1075:1106	malondialdehyde (MDA) production	1075:1106	Moreover, a significant increase in malondialdehyde (MDA) production and a decrease in biofilm formation and metabolic activity of the bacteria was observed in the presence of hydrogel.
33093521	10	62	used	used	1585:1588	arg2	candidate					1606:1614	an excellent candidate	1593:1614	an excellent candidate for periodontitis treatment	1593:1642	Thus, it can be used as an excellent candidate for periodontitis treatment.
33093521	10	62	used	used	1585:1588	arg2	it					1575:1576	it	1575:1576	it	1575:1576	Thus, it can be used as an excellent candidate for periodontitis treatment.
33093521	4	63	theme	thermal	662:668	arg1	stability					670:678	high thermal stability	657:678	high thermal stability	657:678	The proposed material has high thermal stability, controlled release, and water absorption capacity.
34793629	6	0	theme	characteristic	1118:1131	arg1	features					1133:1140	characteristic features	1118:1140	characteristic features	1118:1140	The evaluation of the resultant "papillary" and "reticular" dermal equivalents was based on the analysis of characteristic features of each dermal compartment, such as cell density and deposition of newly synthetized extracellular matrix components in histological sections.
34793629	6	0	theme	characteristic	1118:1131	arg1	deposition					1195:1204	deposition	1195:1204	deposition	1195:1204	The evaluation of the resultant "papillary" and "reticular" dermal equivalents was based on the analysis of characteristic features of each dermal compartment, such as cell density and deposition of newly synthetized extracellular matrix components in histological sections.
34793629	6	0	theme	characteristic	1118:1131	arg1	density					1183:1189	cell density	1178:1189	cell density	1178:1189	The evaluation of the resultant "papillary" and "reticular" dermal equivalents was based on the analysis of characteristic features of each dermal compartment, such as cell density and deposition of newly synthetized extracellular matrix components in histological sections.
34793629	9	1	theme	heparin	2015:2021	arg1	presence					2003:2010	the presence	1999:2010	the presence of heparin	1999:2021	Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).
34793629	2	2	theme	papillary	355:363	arg1	compartments					386:397	papillary and reticular dermal compartments	355:397	papillary and reticular dermal compartments	355:397	This is the first study reporting production of dermal equivalents reproducing features of papillary and reticular dermal compartments.
34793629	2	3	theme	first	276:280	arg1	study					282:286	the first study	272:286	the first study reporting production of dermal equivalents reproducing features of papillary and reticular dermal compartments	272:397	This is the first study reporting production of dermal equivalents reproducing features of papillary and reticular dermal compartments.
34793629	2	3	theme	first	276:280	arg1	This					264:267	This	264:267	This	264:267	This is the first study reporting production of dermal equivalents reproducing features of papillary and reticular dermal compartments.
34793629	9	4	theme	extracellular	1782:1794	arg1	components					1803:1812	distinct extracellular matrix components	1773:1812	distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin)	1773:2022	Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).
34793629	1	5	theme	models	157:162	arg1	lack					149:152	a lack	147:152	a lack of models representing the skin dermal heterogeneity for relevant research and skin engineering applications	147:261	Currently, there is a lack of models representing the skin dermal heterogeneity for relevant research and skin engineering applications.
34793629	5	6	with	crosslinking	865:876	arg1	N-hydroxysuccinimide					941:960	N-hydroxysuccinimide	941:960	N-hydroxysuccinimide (NHS)	941:966	Pore size and hydrolytic stability were controlled by crosslinking with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) or EDC/NHS with covalently bound heparin.
34793629	5	6	with	crosslinking	865:876	arg1	carbodiimide					918:929	1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide	883:929	1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC)	883:935	Pore size and hydrolytic stability were controlled by crosslinking with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) or EDC/NHS with covalently bound heparin.
34793629	5	6	with	crosslinking	865:876	arg1	heparin					1001:1007	covalently bound heparin	984:1007	covalently bound heparin	984:1007	Pore size and hydrolytic stability were controlled by crosslinking with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) or EDC/NHS with covalently bound heparin.
34793629	5	6	with	crosslinking	865:876	arg1	NHS					963:965	NHS	963:965	NHS	963:965	Pore size and hydrolytic stability were controlled by crosslinking with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) or EDC/NHS with covalently bound heparin.
34793629	5	6	with	crosslinking	865:876	arg1	EDC					932:934	EDC	932:934	EDC	932:934	Pore size and hydrolytic stability were controlled by crosslinking with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) or EDC/NHS with covalently bound heparin.
34793629	1	7	theme	dermal	186:191	arg1	heterogeneity					193:205	the skin dermal heterogeneity	177:205	the skin dermal heterogeneity for relevant research and skin engineering applications	177:261	Currently, there is a lack of models representing the skin dermal heterogeneity for relevant research and skin engineering applications.
34793629	3	8	theme	architecture	443:454	arg1	differences					472:482	the architecture and composition differences	439:482	the architecture and composition differences between the papillary and reticular dermis	439:525	Inspired from our current knowledge on the architecture and composition differences between the papillary and reticular dermis, we evaluated different collagen-based porous materials to serve as scaffolds for the three-dimensional expansion of freshly isolated papillary and/or reticular fibroblasts.
34793629	5	9	theme	aminopropyl	905:915	arg1	carbodiimide					918:929	1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide	883:929	1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC)	883:935	Pore size and hydrolytic stability were controlled by crosslinking with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) or EDC/NHS with covalently bound heparin.
34793629	5	9	theme	aminopropyl	905:915	arg1	EDC					932:934	EDC	932:934	EDC	932:934	Pore size and hydrolytic stability were controlled by crosslinking with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) or EDC/NHS with covalently bound heparin.
34793629	8	10	theme	strength	1520:1527	arg1	maintenance					1490:1500	the maintenance	1486:1500	the maintenance of the mechanical strength of the scaffolds during dermal formation	1486:1568	The presence of collagen III seemed to have some positive but non-specific effect only on the maintenance of the mechanical strength of the scaffolds during dermal formation.
34793629	6	11	theme	histological	1262:1273	arg1	sections					1275:1282	histological sections	1262:1282	histological sections	1262:1282	The evaluation of the resultant "papillary" and "reticular" dermal equivalents was based on the analysis of characteristic features of each dermal compartment, such as cell density and deposition of newly synthetized extracellular matrix components in histological sections.
34793629	9	12	theme	carbohydrate	1851:1862	arg1	material					1864:1871	three to five times more carbohydrate material	1826:1871	three to five times more carbohydrate material	1826:1871	Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).
34793629	6	13	theme	components	1248:1257	arg1	deposition					1195:1204	deposition	1195:1204	deposition	1195:1204	The evaluation of the resultant "papillary" and "reticular" dermal equivalents was based on the analysis of characteristic features of each dermal compartment, such as cell density and deposition of newly synthetized extracellular matrix components in histological sections.
34793629	6	13	theme	components	1248:1257	arg1	density					1183:1189	cell density	1178:1189	cell density	1178:1189	The evaluation of the resultant "papillary" and "reticular" dermal equivalents was based on the analysis of characteristic features of each dermal compartment, such as cell density and deposition of newly synthetized extracellular matrix components in histological sections.
34793629	9	14	theme	dermal	1665:1670	arg1	equivalents					1672:1682	dermal equivalents	1665:1682	dermal equivalents reproducing the respective higher papillary than reticular cell densities	1665:1756	Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).
34793629	3	15	theme	reticular	678:686	arg1	fibroblasts					688:698	freshly isolated papillary and/or reticular fibroblasts	644:698	freshly isolated papillary and/or reticular fibroblasts	644:698	Inspired from our current knowledge on the architecture and composition differences between the papillary and reticular dermis, we evaluated different collagen-based porous materials to serve as scaffolds for the three-dimensional expansion of freshly isolated papillary and/or reticular fibroblasts.
34793629	6	16	theme	extracellular	1227:1239	arg1	components					1248:1257	newly synthetized extracellular matrix components	1209:1257	newly synthetized extracellular matrix components	1209:1257	The evaluation of the resultant "papillary" and "reticular" dermal equivalents was based on the analysis of characteristic features of each dermal compartment, such as cell density and deposition of newly synthetized extracellular matrix components in histological sections.
34793629	3	17	theme	papillary	661:669	arg1	fibroblasts					688:698	freshly isolated papillary and/or reticular fibroblasts	644:698	freshly isolated papillary and/or reticular fibroblasts	644:698	Inspired from our current knowledge on the architecture and composition differences between the papillary and reticular dermis, we evaluated different collagen-based porous materials to serve as scaffolds for the three-dimensional expansion of freshly isolated papillary and/or reticular fibroblasts.
34793629	6	18	theme	"	1052:1052	arg1	evaluation					1014:1023	The evaluation	1010:1023	The evaluation of the resultant "papillary" and "reticular" dermal equivalents	1010:1087	The evaluation of the resultant "papillary" and "reticular" dermal equivalents was based on the analysis of characteristic features of each dermal compartment, such as cell density and deposition of newly synthetized extracellular matrix components in histological sections.
34793629	9	19	theme	papillary	1886:1894	arg1	fibroblasts					1896:1906	papillary fibroblasts	1886:1906	papillary fibroblasts	1886:1906	Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).
34793629	0	20	theme	human	72:76	arg1	growth					119:124	human dermal fibroblast subpopulations' tissue growth	72:124	human dermal fibroblast subpopulations' tissue growth	72:124	Benefit of coupling heparin to crosslinked collagen I/III scaffolds for human dermal fibroblast subpopulations' tissue growth.
34793629	3	21	from	knowledge	426:434	arg1	differences					472:482	the architecture and composition differences	439:482	the architecture and composition differences between the papillary and reticular dermis	439:525	Inspired from our current knowledge on the architecture and composition differences between the papillary and reticular dermis, we evaluated different collagen-based porous materials to serve as scaffolds for the three-dimensional expansion of freshly isolated papillary and/or reticular fibroblasts.
34793629	8	22	theme	collagen	1412:1419	arg1	presence					1400:1407	The presence	1396:1407	The presence of collagen III	1396:1423	The presence of collagen III seemed to have some positive but non-specific effect only on the maintenance of the mechanical strength of the scaffolds during dermal formation.
34793629	0	23	theme	fibroblast	85:94	arg1	growth					119:124	human dermal fibroblast subpopulations' tissue growth	72:124	human dermal fibroblast subpopulations' tissue growth	72:124	Benefit of coupling heparin to crosslinked collagen I/III scaffolds for human dermal fibroblast subpopulations' tissue growth.
34793629	6	24	dep	"	1052:1052	arg1	equivalents					1077:1087	dermal equivalents	1070:1087	dermal equivalents	1070:1087	The evaluation of the resultant "papillary" and "reticular" dermal equivalents was based on the analysis of characteristic features of each dermal compartment, such as cell density and deposition of newly synthetized extracellular matrix components in histological sections.
34793629	6	24	dep	"	1052:1052	arg1	the					1028:1030	the	1028:1030	the	1028:1030	The evaluation of the resultant "papillary" and "reticular" dermal equivalents was based on the analysis of characteristic features of each dermal compartment, such as cell density and deposition of newly synthetized extracellular matrix components in histological sections.
34793629	9	25	dep	components	1803:1812	arg1	deposition					1814:1823	deposition	1814:1823	distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin)	1773:2022	Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).
34793629	4	26	dep	collagen	735:742	arg1	mixtures					768:775	mixtures	768:775	mixtures	768:775	The scaffolds, composed of either collagen I or collagen I and III mixtures, were prepared by lyophilization.
34793629	2	27	theme	reticular	369:377	arg1	compartments					386:397	papillary and reticular dermal compartments	355:397	papillary and reticular dermal compartments	355:397	This is the first study reporting production of dermal equivalents reproducing features of papillary and reticular dermal compartments.
34793629	8	28	theme	positive	1445:1452	arg1	effect					1471:1476	some positive but non-specific effect	1440:1476	some positive but non-specific effect	1440:1476	The presence of collagen III seemed to have some positive but non-specific effect only on the maintenance of the mechanical strength of the scaffolds during dermal formation.
34793629	0	29	theme	coupling	11:18	arg1	heparin					20:26	coupling heparin	11:26	coupling heparin	11:26	Benefit of coupling heparin to crosslinked collagen I/III scaffolds for human dermal fibroblast subpopulations' tissue growth.
34793629	7	30	theme	fibroblast	1370:1379	arg1	subpopulation					1381:1393	the fibroblast subpopulation	1366:1393	the fibroblast subpopulation	1366:1393	Crosslinking supported cell growth during dermal tissue formation independent on the fibroblast subpopulation.
34793629	9	31	theme	collagen	1958:1965	arg1	production					1967:1976	higher collagen production	1951:1976	higher collagen production	1951:1976	Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).
34793629	6	32	theme	dermal	1070:1075	arg1	equivalents					1077:1087	dermal equivalents	1070:1087	dermal equivalents	1070:1087	The evaluation of the resultant "papillary" and "reticular" dermal equivalents was based on the analysis of characteristic features of each dermal compartment, such as cell density and deposition of newly synthetized extracellular matrix components in histological sections.
34793629	8	33	theme	non-specific	1458:1469	arg1	effect					1471:1476	some positive but non-specific effect	1440:1476	some positive but non-specific effect	1440:1476	The presence of collagen III seemed to have some positive but non-specific effect only on the maintenance of the mechanical strength of the scaffolds during dermal formation.
34793629	5	34	theme	hydrolytic	825:834	arg1	stability					836:844	hydrolytic stability	825:844	hydrolytic stability	825:844	Pore size and hydrolytic stability were controlled by crosslinking with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) or EDC/NHS with covalently bound heparin.
34793629	9	35	dep	deposition	1814:1823	arg1	deposited					1873:1881	deposited	1873:1881	deposited	1873:1881	Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).
34793629	6	36	theme	"	1068:1068	arg1	evaluation					1014:1023	The evaluation	1010:1023	The evaluation of the resultant "papillary" and "reticular" dermal equivalents	1010:1087	The evaluation of the resultant "papillary" and "reticular" dermal equivalents was based on the analysis of characteristic features of each dermal compartment, such as cell density and deposition of newly synthetized extracellular matrix components in histological sections.
34793629	0	37	theme	collagen	43:50	arg1	I/III					52:56	crosslinked collagen I/III	31:56	crosslinked collagen I/III	31:56	Benefit of coupling heparin to crosslinked collagen I/III scaffolds for human dermal fibroblast subpopulations' tissue growth.
34793629	9	38	theme	higher	1711:1716	arg1	papillary					1718:1726	the respective higher papillary	1696:1726	the respective higher papillary than reticular cell densities	1696:1756	Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).
34793629	6	39	theme	compartment	1157:1167	arg1	features					1133:1140	characteristic features	1118:1140	characteristic features	1118:1140	The evaluation of the resultant "papillary" and "reticular" dermal equivalents was based on the analysis of characteristic features of each dermal compartment, such as cell density and deposition of newly synthetized extracellular matrix components in histological sections.
34793629	6	39	theme	compartment	1157:1167	arg1	deposition					1195:1204	deposition	1195:1204	deposition	1195:1204	The evaluation of the resultant "papillary" and "reticular" dermal equivalents was based on the analysis of characteristic features of each dermal compartment, such as cell density and deposition of newly synthetized extracellular matrix components in histological sections.
34793629	6	39	theme	compartment	1157:1167	arg1	density					1183:1189	cell density	1178:1189	cell density	1178:1189	The evaluation of the resultant "papillary" and "reticular" dermal equivalents was based on the analysis of characteristic features of each dermal compartment, such as cell density and deposition of newly synthetized extracellular matrix components in histological sections.
34793629	1	40	theme	relevant	211:218	arg1	research					220:227	relevant research	211:227	relevant research	211:227	Currently, there is a lack of models representing the skin dermal heterogeneity for relevant research and skin engineering applications.
34793629	7	41	theme	dermal	1327:1332	arg1	formation					1341:1349	dermal tissue formation	1327:1349	dermal tissue formation independent on the fibroblast subpopulation	1327:1393	Crosslinking supported cell growth during dermal tissue formation independent on the fibroblast subpopulation.
34793629	3	42	theme	different	541:549	arg1	materials					573:581	different collagen-based porous materials	541:581	different collagen-based porous materials	541:581	Inspired from our current knowledge on the architecture and composition differences between the papillary and reticular dermis, we evaluated different collagen-based porous materials to serve as scaffolds for the three-dimensional expansion of freshly isolated papillary and/or reticular fibroblasts.
34793629	9	43	theme	cell	1743:1746	arg1	densities					1748:1756	reticular cell densities	1733:1756	reticular cell densities	1733:1756	Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).
34793629	6	44	theme	features	1133:1140	arg1	analysis					1106:1113	the analysis	1102:1113	the analysis of characteristic features of each dermal compartment, such as cell density and deposition of newly synthetized extracellular matrix components in histological sections	1102:1282	The evaluation of the resultant "papillary" and "reticular" dermal equivalents was based on the analysis of characteristic features of each dermal compartment, such as cell density and deposition of newly synthetized extracellular matrix components in histological sections.
34793629	5	45	theme	Pore	811:814	arg1	size					816:819	Pore size	811:819	Pore size	811:819	Pore size and hydrolytic stability were controlled by crosslinking with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) or EDC/NHS with covalently bound heparin.
34793629	1	46	theme	engineering	238:248	arg1	applications					250:261	skin engineering applications	233:261	skin engineering applications	233:261	Currently, there is a lack of models representing the skin dermal heterogeneity for relevant research and skin engineering applications.
34793629	9	47	contain	containing	1925:1934	arg2	heparin					1936:1942	heparin	1936:1942	heparin	1936:1942	Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).
34793629	9	47	contain	containing	1925:1934	arg1	scaffolds					1915:1923	all scaffolds	1911:1923	all scaffolds containing heparin	1911:1942	Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).
34793629	9	48	theme	distinct	1773:1780	arg1	components					1803:1812	distinct extracellular matrix components	1773:1812	distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin)	1773:2022	Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).
34793629	3	49	theme	porous	566:571	arg1	materials					573:581	different collagen-based porous materials	541:581	different collagen-based porous materials	541:581	Inspired from our current knowledge on the architecture and composition differences between the papillary and reticular dermis, we evaluated different collagen-based porous materials to serve as scaffolds for the three-dimensional expansion of freshly isolated papillary and/or reticular fibroblasts.
34793629	9	50	theme	matrix	1796:1801	arg1	components					1803:1812	distinct extracellular matrix components	1773:1812	distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin)	1773:2022	Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).
34793629	9	51	dep	times	1840:1844	arg1	to					1832:1833	to	1832:1833	to	1832:1833	Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).
34793629	9	51	dep	times	1840:1844	arg1	more					1846:1849	more	1846:1849	more	1846:1849	Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).
34793629	2	52	theme	dermal	312:317	arg1	equivalents					319:329	dermal equivalents	312:329	dermal equivalents reproducing features of papillary and reticular dermal compartments	312:397	This is the first study reporting production of dermal equivalents reproducing features of papillary and reticular dermal compartments.
34793629	0	53	link	crosslinked	31:41	arg1	I/III					52:56	crosslinked collagen I/III	31:56	crosslinked collagen I/III	31:56	Benefit of coupling heparin to crosslinked collagen I/III scaffolds for human dermal fibroblast subpopulations' tissue growth.
34793629	6	54	theme	cell	1178:1181	arg1	density					1183:1189	cell density	1178:1189	cell density	1178:1189	The evaluation of the resultant "papillary" and "reticular" dermal equivalents was based on the analysis of characteristic features of each dermal compartment, such as cell density and deposition of newly synthetized extracellular matrix components in histological sections.
34793629	1	55	theme	skin	181:184	arg1	heterogeneity					193:205	the skin dermal heterogeneity	177:205	the skin dermal heterogeneity for relevant research and skin engineering applications	177:261	Currently, there is a lack of models representing the skin dermal heterogeneity for relevant research and skin engineering applications.
34793629	9	56	theme	three	1826:1830	arg1	times					1840:1844	times	1840:1844	times	1840:1844	Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).
34793629	8	57	theme	mechanical	1509:1518	arg1	strength					1520:1527	the mechanical strength	1505:1527	the mechanical strength of the scaffolds during dermal formation	1505:1568	The presence of collagen III seemed to have some positive but non-specific effect only on the maintenance of the mechanical strength of the scaffolds during dermal formation.
34793629	5	58	theme	3-dimethyl	894:903	arg1	carbodiimide					918:929	1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide	883:929	1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC)	883:935	Pore size and hydrolytic stability were controlled by crosslinking with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) or EDC/NHS with covalently bound heparin.
34793629	5	58	theme	3-dimethyl	894:903	arg1	EDC					932:934	EDC	932:934	EDC	932:934	Pore size and hydrolytic stability were controlled by crosslinking with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) or EDC/NHS with covalently bound heparin.
34793629	9	59	theme	five	1835:1838	arg1	times					1840:1844	times	1840:1844	times	1840:1844	Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).
34793629	3	60	theme	three-dimensional	613:629	arg1	expansion					631:639	the three-dimensional expansion	609:639	the three-dimensional expansion of freshly isolated papillary and/or reticular fibroblasts	609:698	Inspired from our current knowledge on the architecture and composition differences between the papillary and reticular dermis, we evaluated different collagen-based porous materials to serve as scaffolds for the three-dimensional expansion of freshly isolated papillary and/or reticular fibroblasts.
34793629	8	61	theme	scaffolds	1536:1544	arg1	strength					1520:1527	the mechanical strength	1505:1527	the mechanical strength of the scaffolds during dermal formation	1505:1568	The presence of collagen III seemed to have some positive but non-specific effect only on the maintenance of the mechanical strength of the scaffolds during dermal formation.
34793629	9	62	theme	Histological	1571:1582	arg1	analyses					1584:1591	Histological analyses	1571:1591	Histological analyses	1571:1591	Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).
34793629	6	63	theme	reticular	1059:1067	arg1	"					1068:1068	"reticular"	1058:1068	"reticular"	1058:1068	The evaluation of the resultant "papillary" and "reticular" dermal equivalents was based on the analysis of characteristic features of each dermal compartment, such as cell density and deposition of newly synthetized extracellular matrix components in histological sections.
34793629	8	64	theme	dermal	1553:1558	arg1	formation					1560:1568	dermal formation	1553:1568	dermal formation	1553:1568	The presence of collagen III seemed to have some positive but non-specific effect only on the maintenance of the mechanical strength of the scaffolds during dermal formation.
34793629	6	65	theme	matrix	1241:1246	arg1	components					1248:1257	newly synthetized extracellular matrix components	1209:1257	newly synthetized extracellular matrix components	1209:1257	The evaluation of the resultant "papillary" and "reticular" dermal equivalents was based on the analysis of characteristic features of each dermal compartment, such as cell density and deposition of newly synthetized extracellular matrix components in histological sections.
34793629	3	66	theme	isolated	652:659	arg1	fibroblasts					688:698	freshly isolated papillary and/or reticular fibroblasts	644:698	freshly isolated papillary and/or reticular fibroblasts	644:698	Inspired from our current knowledge on the architecture and composition differences between the papillary and reticular dermis, we evaluated different collagen-based porous materials to serve as scaffolds for the three-dimensional expansion of freshly isolated papillary and/or reticular fibroblasts.
34793629	5	67	theme	1-ethyl-3-	883:892	arg1	carbodiimide					918:929	1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide	883:929	1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC)	883:935	Pore size and hydrolytic stability were controlled by crosslinking with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) or EDC/NHS with covalently bound heparin.
34793629	5	67	theme	1-ethyl-3-	883:892	arg1	EDC					932:934	EDC	932:934	EDC	932:934	Pore size and hydrolytic stability were controlled by crosslinking with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) or EDC/NHS with covalently bound heparin.
34793629	6	68	theme	synthetized	1215:1225	arg1	components					1248:1257	newly synthetized extracellular matrix components	1209:1257	newly synthetized extracellular matrix components	1209:1257	The evaluation of the resultant "papillary" and "reticular" dermal equivalents was based on the analysis of characteristic features of each dermal compartment, such as cell density and deposition of newly synthetized extracellular matrix components in histological sections.
34793629	9	69	dep	material	1864:1871	arg1	times					1840:1844	times	1840:1844	times	1840:1844	Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).
34793629	0	70	theme	dermal	78:83	arg1	growth					119:124	human dermal fibroblast subpopulations' tissue growth	72:124	human dermal fibroblast subpopulations' tissue growth	72:124	Benefit of coupling heparin to crosslinked collagen I/III scaffolds for human dermal fibroblast subpopulations' tissue growth.
34793629	9	71	theme	significant	1608:1618	arg1	effect					1633:1638	a significant and specific effect	1606:1638	a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin)	1606:2022	Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).
34793629	6	72	theme	papillary	1043:1051	arg1	"					1052:1052	papillary"	1043:1052	papillary"	1043:1052	The evaluation of the resultant "papillary" and "reticular" dermal equivalents was based on the analysis of characteristic features of each dermal compartment, such as cell density and deposition of newly synthetized extracellular matrix components in histological sections.
34793629	8	73	contain	have	1435:1438	arg2	effect					1471:1476	some positive but non-specific effect	1440:1476	some positive but non-specific effect	1440:1476	The presence of collagen III seemed to have some positive but non-specific effect only on the maintenance of the mechanical strength of the scaffolds during dermal formation.
34793629	8	73	contain	have	1435:1438	arg1	presence					1400:1407	The presence	1396:1407	The presence of collagen III	1396:1423	The presence of collagen III seemed to have some positive but non-specific effect only on the maintenance of the mechanical strength of the scaffolds during dermal formation.
34793629	2	74	theme	dermal	379:384	arg1	compartments					386:397	papillary and reticular dermal compartments	355:397	papillary and reticular dermal compartments	355:397	This is the first study reporting production of dermal equivalents reproducing features of papillary and reticular dermal compartments.
34793629	9	75	theme	specific	1624:1631	arg1	effect					1633:1638	a significant and specific effect	1606:1638	a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin)	1606:2022	Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).
34793629	6	76	theme	resultant	1032:1040	arg1	"					1052:1052	papillary"	1043:1052	papillary"	1043:1052	The evaluation of the resultant "papillary" and "reticular" dermal equivalents was based on the analysis of characteristic features of each dermal compartment, such as cell density and deposition of newly synthetized extracellular matrix components in histological sections.
34793629	6	77	from	deposition	1195:1204	arg1	sections					1275:1282	histological sections	1262:1282	histological sections	1262:1282	The evaluation of the resultant "papillary" and "reticular" dermal equivalents was based on the analysis of characteristic features of each dermal compartment, such as cell density and deposition of newly synthetized extracellular matrix components in histological sections.
34793629	2	78	theme	equivalents	319:329	arg1	production					298:307	production	298:307	production of dermal equivalents reproducing features of papillary and reticular dermal compartments	298:397	This is the first study reporting production of dermal equivalents reproducing features of papillary and reticular dermal compartments.
34793629	0	79	theme	tissue	112:117	arg1	growth					119:124	human dermal fibroblast subpopulations' tissue growth	72:124	human dermal fibroblast subpopulations' tissue growth	72:124	Benefit of coupling heparin to crosslinked collagen I/III scaffolds for human dermal fibroblast subpopulations' tissue growth.
34793629	5	80	with	EDC/NHS	971:977	arg1	N-hydroxysuccinimide					941:960	N-hydroxysuccinimide	941:960	N-hydroxysuccinimide (NHS)	941:966	Pore size and hydrolytic stability were controlled by crosslinking with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) or EDC/NHS with covalently bound heparin.
34793629	5	80	with	EDC/NHS	971:977	arg1	carbodiimide					918:929	1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide	883:929	1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC)	883:935	Pore size and hydrolytic stability were controlled by crosslinking with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) or EDC/NHS with covalently bound heparin.
34793629	5	80	with	EDC/NHS	971:977	arg1	heparin					1001:1007	covalently bound heparin	984:1007	covalently bound heparin	984:1007	Pore size and hydrolytic stability were controlled by crosslinking with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) or EDC/NHS with covalently bound heparin.
34793629	5	80	with	EDC/NHS	971:977	arg1	NHS					963:965	NHS	963:965	NHS	963:965	Pore size and hydrolytic stability were controlled by crosslinking with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) or EDC/NHS with covalently bound heparin.
34793629	5	80	with	EDC/NHS	971:977	arg1	EDC					932:934	EDC	932:934	EDC	932:934	Pore size and hydrolytic stability were controlled by crosslinking with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) or EDC/NHS with covalently bound heparin.
34793629	3	81	theme	composition	460:470	arg1	differences					472:482	the architecture and composition differences	439:482	the architecture and composition differences between the papillary and reticular dermis	439:525	Inspired from our current knowledge on the architecture and composition differences between the papillary and reticular dermis, we evaluated different collagen-based porous materials to serve as scaffolds for the three-dimensional expansion of freshly isolated papillary and/or reticular fibroblasts.
34793629	2	82	theme	compartments	386:397	arg1	features					343:350	features	343:350	features of papillary and reticular dermal compartments	343:397	This is the first study reporting production of dermal equivalents reproducing features of papillary and reticular dermal compartments.
34793629	0	83	theme	heparin	20:26	arg1	Benefit					0:6	Benefit	0:6	Benefit of coupling heparin to crosslinked collagen I/III	0:56	Benefit of coupling heparin to crosslinked collagen I/III scaffolds for human dermal fibroblast subpopulations' tissue growth.
34793629	9	84	theme	reticular	1733:1741	arg1	densities					1748:1756	reticular cell densities	1733:1756	reticular cell densities	1733:1756	Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).
34793629	5	85	theme	bound	995:999	arg1	heparin					1001:1007	covalently bound heparin	984:1007	covalently bound heparin	984:1007	Pore size and hydrolytic stability were controlled by crosslinking with 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS) or EDC/NHS with covalently bound heparin.
34793629	9	86	theme	higher	1951:1956	arg1	production					1967:1976	higher collagen production	1951:1976	higher collagen production	1951:1976	Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).
34793629	6	87	from	density	1183:1189	arg1	sections					1275:1282	histological sections	1262:1282	histological sections	1262:1282	The evaluation of the resultant "papillary" and "reticular" dermal equivalents was based on the analysis of characteristic features of each dermal compartment, such as cell density and deposition of newly synthetized extracellular matrix components in histological sections.
34793629	3	88	theme	papillary	496:504	arg1	dermis					520:525	the papillary and reticular dermis	492:525	the papillary and reticular dermis	492:525	Inspired from our current knowledge on the architecture and composition differences between the papillary and reticular dermis, we evaluated different collagen-based porous materials to serve as scaffolds for the three-dimensional expansion of freshly isolated papillary and/or reticular fibroblasts.
34793629	0	89	theme	crosslinked	31:41	arg1	I/III					52:56	crosslinked collagen I/III	31:56	crosslinked collagen I/III	31:56	Benefit of coupling heparin to crosslinked collagen I/III scaffolds for human dermal fibroblast subpopulations' tissue growth.
34793629	7	90	theme	independent	1351:1361	arg1	formation					1341:1349	dermal tissue formation	1327:1349	dermal tissue formation independent on the fibroblast subpopulation	1327:1393	Crosslinking supported cell growth during dermal tissue formation independent on the fibroblast subpopulation.
34793629	3	91	theme	fibroblasts	688:698	arg1	expansion					631:639	the three-dimensional expansion	609:639	the three-dimensional expansion of freshly isolated papillary and/or reticular fibroblasts	609:698	Inspired from our current knowledge on the architecture and composition differences between the papillary and reticular dermis, we evaluated different collagen-based porous materials to serve as scaffolds for the three-dimensional expansion of freshly isolated papillary and/or reticular fibroblasts.
34793629	9	92	theme	respective	1700:1709	arg1	papillary					1718:1726	the respective higher papillary	1696:1726	the respective higher papillary than reticular cell densities	1696:1756	Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).
34793629	7	93	theme	tissue	1334:1339	arg1	formation					1341:1349	dermal tissue formation	1327:1349	dermal tissue formation independent on the fibroblast subpopulation	1327:1393	Crosslinking supported cell growth during dermal tissue formation independent on the fibroblast subpopulation.
34793629	3	94	theme	current	418:424	arg1	knowledge					426:434	our current knowledge	414:434	our current knowledge on the architecture and composition differences between the papillary and reticular dermis	414:525	Inspired from our current knowledge on the architecture and composition differences between the papillary and reticular dermis, we evaluated different collagen-based porous materials to serve as scaffolds for the three-dimensional expansion of freshly isolated papillary and/or reticular fibroblasts.
34793629	6	95	theme	dermal	1150:1155	arg1	compartment					1157:1167	each dermal compartment	1145:1167	each dermal compartment	1145:1167	The evaluation of the resultant "papillary" and "reticular" dermal equivalents was based on the analysis of characteristic features of each dermal compartment, such as cell density and deposition of newly synthetized extracellular matrix components in histological sections.
34793629	9	96	theme	heparin	1643:1649	arg1	effect					1633:1638	a significant and specific effect	1606:1638	a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin)	1606:2022	Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).
34793629	3	97	theme	reticular	510:518	arg1	dermis					520:525	the papillary and reticular dermis	492:525	the papillary and reticular dermis	492:525	Inspired from our current knowledge on the architecture and composition differences between the papillary and reticular dermis, we evaluated different collagen-based porous materials to serve as scaffolds for the three-dimensional expansion of freshly isolated papillary and/or reticular fibroblasts.
34793629	9	98	located	observed	1982:1989	arg2	production					1967:1976	higher collagen production	1951:1976	higher collagen production	1951:1976	Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).
34793629	9	98	located	observed	1982:1989	arg1	presence					2003:2010	the presence	1999:2010	the presence of heparin	1999:2021	Histological analyses demonstrated a significant and specific effect of heparin on generating dermal equivalents reproducing the respective higher papillary than reticular cell densities and supporting distinct extracellular matrix components deposition (three to five times more carbohydrate material deposited by papillary fibroblasts in all scaffolds containing heparin, while higher collagen production was observed only in the presence of heparin).
34793629	7	99	theme	cell	1308:1311	arg1	growth					1313:1318	cell growth	1308:1318	cell growth	1308:1318	Crosslinking supported cell growth during dermal tissue formation independent on the fibroblast subpopulation.
34793629	3	100	theme	collagen-based	551:564	arg1	materials					573:581	different collagen-based porous materials	541:581	different collagen-based porous materials	541:581	Inspired from our current knowledge on the architecture and composition differences between the papillary and reticular dermis, we evaluated different collagen-based porous materials to serve as scaffolds for the three-dimensional expansion of freshly isolated papillary and/or reticular fibroblasts.
34793629	1	101	theme	skin	233:236	arg1	applications					250:261	skin engineering applications	233:261	skin engineering applications	233:261	Currently, there is a lack of models representing the skin dermal heterogeneity for relevant research and skin engineering applications.
31923752	1	0	from	samples	354:360	arg1	microextraction					293:307	the microextraction	289:307	the microextraction of endocrine disrupting compounds from water samples	289:360	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	1	0	from	samples	354:360	arg1	compounds					333:341	endocrine disrupting compounds	312:341	endocrine disrupting compounds from water samples	312:360	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	5	1	theme	equilibrium	1341:1351	arg1	models					1353:1358	pseudo-second order kinetics and Langmuir equilibrium models	1299:1358	pseudo-second order kinetics and Langmuir equilibrium models	1299:1358	The kinetic and equilibrium studies revealed that the adsorption process follow pseudo-second order kinetics and Langmuir equilibrium models.
31923752	7	2	theme	disrupting	1653:1662	arg1	compounds					1664:1672	both endocrine disrupting compounds	1638:1672	both endocrine disrupting compounds	1638:1672	Different solvents used for microextraction and maximum extraction of both endocrine disrupting compounds were obtained with methanol.
31923752	2	3	theme	X-ray	669:673	arg1	diffraction					675:685	X-ray diffraction	669:685	X-ray diffraction for phase determination	669:709	The beads were characterized using spectroscopic techniques, such as Fourier transform infra-red spectroscopy for the determination of different functional groups, Scanning electron microscopy for surface morphology, X-ray diffraction for phase determination and energy dispersive X ray spectroscopy for elemental composition.
31923752	6	4	theme	epichlorohydrin	1426:1440	arg1	microextraction					1407:1421	the microextraction	1403:1421	the microextraction of epichlorohydrin	1403:1440	Analytical parameters were calculated for the microextraction of epichlorohydrin and bisphenol A. Limit of detection was 8.25 ngL-1 and 13.99 ngL-1 (n = 4) for epichlorohydrin and bisphenol A, respectively.
31923752	2	5	theme	energy	715:720	arg1	spectroscopy					739:750	energy dispersive X ray spectroscopy	715:750	energy dispersive X ray spectroscopy	715:750	The beads were characterized using spectroscopic techniques, such as Fourier transform infra-red spectroscopy for the determination of different functional groups, Scanning electron microscopy for surface morphology, X-ray diffraction for phase determination and energy dispersive X ray spectroscopy for elemental composition.
31923752	1	6	theme	alginate	191:198	arg1	adsorbent					275:283	adsorbent	275:283	adsorbent	275:283	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	1	6	theme	alginate	191:198	arg1	beads					237:241	crosslinked alginate encapsulated magnetic graphene oxide beads	179:241	crosslinked alginate encapsulated magnetic graphene oxide beads	179:241	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	9	7	theme	graphene	1864:1871	arg1	oxide					1873:1877	The magnetic graphene oxide	1851:1877	The magnetic graphene oxide encapsulated inside an alginate	1851:1909	The magnetic graphene oxide encapsulated inside an alginate shows nontoxic green chemical with high extraction performance for toxic organic compounds in water treatment.
31923752	7	8	theme	Different	1568:1576	arg1	solvents					1578:1585	Different solvents	1568:1585	Different solvents used for microextraction and maximum extraction of both endocrine disrupting compounds	1568:1672	Different solvents used for microextraction and maximum extraction of both endocrine disrupting compounds were obtained with methanol.
31923752	3	9	theme	alginate	845:852	arg1	alginate					845:852	alginate	845:852	alginate	845:852	The results revealed that beads surface have functional groups of alginate and graphene oxide which are involved in π-π, n-πinteractions and hydrogen bonding for the bisphenol A and epichlorohydrin adsorption.
31923752	3	9	theme	alginate	845:852	arg1	oxide					867:871	graphene oxide	858:871	graphene oxide	858:871	The results revealed that beads surface have functional groups of alginate and graphene oxide which are involved in π-π, n-πinteractions and hydrogen bonding for the bisphenol A and epichlorohydrin adsorption.
31923752	3	9	theme	alginate	845:852	arg1	groups					835:840	functional groups	824:840	functional groups of alginate and graphene oxide which are involved in π-π, n-πinteractions and hydrogen bonding for the bisphenol A and epichlorohydrin adsorption	824:986	The results revealed that beads surface have functional groups of alginate and graphene oxide which are involved in π-π, n-πinteractions and hydrogen bonding for the bisphenol A and epichlorohydrin adsorption.
31923752	1	10	theme	magnetic	213:220	arg1	adsorbent					275:283	adsorbent	275:283	adsorbent	275:283	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	1	10	theme	magnetic	213:220	arg1	beads					237:241	crosslinked alginate encapsulated magnetic graphene oxide beads	179:241	crosslinked alginate encapsulated magnetic graphene oxide beads	179:241	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	2	11	theme	electron	625:632	arg1	microscopy					634:643	Scanning electron microscopy	616:643	Scanning electron microscopy for surface morphology	616:666	The beads were characterized using spectroscopic techniques, such as Fourier transform infra-red spectroscopy for the determination of different functional groups, Scanning electron microscopy for surface morphology, X-ray diffraction for phase determination and energy dispersive X ray spectroscopy for elemental composition.
31923752	0	12	theme	water	136:140	arg1	samples					142:148	water samples	136:148	water samples	136:148	Eco-friendly alginate encapsulated magnetic graphene oxide beads for solid phase microextraction of endocrine disrupting compounds from water samples.
31923752	1	13	theme	oxide	231:235	arg1	adsorbent					275:283	adsorbent	275:283	adsorbent	275:283	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	1	13	theme	oxide	231:235	arg1	beads					237:241	crosslinked alginate encapsulated magnetic graphene oxide beads	179:241	crosslinked alginate encapsulated magnetic graphene oxide beads	179:241	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	4	14	theme	endocrine	1038:1046	arg1	A					1099:1099	Epichlorohydrin ad Bisphenol A	1070:1099	Epichlorohydrin ad Bisphenol A	1070:1099	The experimental conditions were studied for two endocrine disrupting compounds (Epichlorohydrin ad Bisphenol A) and at optimum conditions the adsorption capacity was 6.73 mgg-1 for epichlorohydrin and 7.01 mgg-1 for bisphenol A.
31923752	4	14	theme	endocrine	1038:1046	arg1	compounds					1059:1067	two endocrine disrupting compounds	1034:1067	two endocrine disrupting compounds (Epichlorohydrin ad Bisphenol A)	1034:1100	The experimental conditions were studied for two endocrine disrupting compounds (Epichlorohydrin ad Bisphenol A) and at optimum conditions the adsorption capacity was 6.73 mgg-1 for epichlorohydrin and 7.01 mgg-1 for bisphenol A.
31923752	0	15	from	microextraction	81:95	arg1	samples					142:148	water samples	136:148	water samples	136:148	Eco-friendly alginate encapsulated magnetic graphene oxide beads for solid phase microextraction of endocrine disrupting compounds from water samples.
31923752	4	16	theme	experimental	993:1004	arg1	conditions					1006:1015	The experimental conditions	989:1015	The experimental conditions	989:1015	The experimental conditions were studied for two endocrine disrupting compounds (Epichlorohydrin ad Bisphenol A) and at optimum conditions the adsorption capacity was 6.73 mgg-1 for epichlorohydrin and 7.01 mgg-1 for bisphenol A.
31923752	3	17	theme	graphene	858:865	arg1	oxide					867:871	graphene oxide	858:871	graphene oxide	858:871	The results revealed that beads surface have functional groups of alginate and graphene oxide which are involved in π-π, n-πinteractions and hydrogen bonding for the bisphenol A and epichlorohydrin adsorption.
31923752	6	18	theme	detection	1468:1476	arg1	Limit					1459:1463	bisphenol A. Limit	1446:1463	bisphenol A. Limit of detection	1446:1476	Analytical parameters were calculated for the microextraction of epichlorohydrin and bisphenol A. Limit of detection was 8.25 ngL-1 and 13.99 ngL-1 (n = 4) for epichlorohydrin and bisphenol A, respectively.
31923752	3	19	theme	bisphenol	945:953	arg1	A					955:955	bisphenol A	945:955	bisphenol A	945:955	The results revealed that beads surface have functional groups of alginate and graphene oxide which are involved in π-π, n-πinteractions and hydrogen bonding for the bisphenol A and epichlorohydrin adsorption.
31923752	4	20	theme	Bisphenol	1089:1097	arg1	A					1099:1099	Epichlorohydrin ad Bisphenol A	1070:1099	Epichlorohydrin ad Bisphenol A	1070:1099	The experimental conditions were studied for two endocrine disrupting compounds (Epichlorohydrin ad Bisphenol A) and at optimum conditions the adsorption capacity was 6.73 mgg-1 for epichlorohydrin and 7.01 mgg-1 for bisphenol A.
31923752	4	20	theme	Bisphenol	1089:1097	arg1	compounds					1059:1067	two endocrine disrupting compounds	1034:1067	two endocrine disrupting compounds (Epichlorohydrin ad Bisphenol A)	1034:1100	The experimental conditions were studied for two endocrine disrupting compounds (Epichlorohydrin ad Bisphenol A) and at optimum conditions the adsorption capacity was 6.73 mgg-1 for epichlorohydrin and 7.01 mgg-1 for bisphenol A.
31923752	0	21	theme	solid	69:73	arg1	microextraction					81:95	solid phase microextraction	69:95	solid phase microextraction of endocrine disrupting compounds from water samples	69:148	Eco-friendly alginate encapsulated magnetic graphene oxide beads for solid phase microextraction of endocrine disrupting compounds from water samples.
31923752	5	22	theme	equilibrium	1235:1245	arg1	studies					1247:1253	The kinetic and equilibrium studies	1219:1253	studies	1247:1253	The kinetic and equilibrium studies revealed that the adsorption process follow pseudo-second order kinetics and Langmuir equilibrium models.
31923752	1	23	theme	proposed	158:165	arg1	iron					174:177	the proposed method iron	154:177	the proposed method iron	154:177	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	9	24	theme	extraction	1951:1960	arg1	performance					1962:1972	high extraction performance	1946:1972	high extraction performance for toxic organic compounds in water treatment	1946:2019	The magnetic graphene oxide encapsulated inside an alginate shows nontoxic green chemical with high extraction performance for toxic organic compounds in water treatment.
31923752	1	25	theme	endocrine	312:320	arg1	compounds					333:341	endocrine disrupting compounds	312:341	endocrine disrupting compounds from water samples	312:360	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	3	26	theme	hydrogen	920:927	arg1	bonding					929:935	hydrogen bonding	920:935	hydrogen bonding	920:935	The results revealed that beads surface have functional groups of alginate and graphene oxide which are involved in π-π, n-πinteractions and hydrogen bonding for the bisphenol A and epichlorohydrin adsorption.
31923752	2	27	theme	functional	597:606	arg1	groups					608:613	different functional groups	587:613	different functional groups	587:613	The beads were characterized using spectroscopic techniques, such as Fourier transform infra-red spectroscopy for the determination of different functional groups, Scanning electron microscopy for surface morphology, X-ray diffraction for phase determination and energy dispersive X ray spectroscopy for elemental composition.
31923752	2	28	theme	ray	735:737	arg1	spectroscopy					739:750	energy dispersive X ray spectroscopy	715:750	energy dispersive X ray spectroscopy	715:750	The beads were characterized using spectroscopic techniques, such as Fourier transform infra-red spectroscopy for the determination of different functional groups, Scanning electron microscopy for surface morphology, X-ray diffraction for phase determination and energy dispersive X ray spectroscopy for elemental composition.
31923752	0	29	theme	endocrine	100:108	arg1	compounds					121:129	endocrine disrupting compounds	100:129	endocrine disrupting compounds from water samples	100:148	Eco-friendly alginate encapsulated magnetic graphene oxide beads for solid phase microextraction of endocrine disrupting compounds from water samples.
31923752	1	30	theme	high	386:389	arg1	chromatography					410:423	high performance liquid chromatography	386:423	high performance liquid chromatography with ultraviolet detector	386:449	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	2	31	dep	Fourier	521:527	arg1	transform					529:537	transform	529:537	transform infra-red spectroscopy for the determination of different functional groups, Scanning electron microscopy for surface morphology, X-ray diffraction for phase determination and energy dispersive X ray spectroscopy for elemental composition	529:776	The beads were characterized using spectroscopic techniques, such as Fourier transform infra-red spectroscopy for the determination of different functional groups, Scanning electron microscopy for surface morphology, X-ray diffraction for phase determination and energy dispersive X ray spectroscopy for elemental composition.
31923752	4	32	theme	Epichlorohydrin	1070:1084	arg1	A					1099:1099	Epichlorohydrin ad Bisphenol A	1070:1099	Epichlorohydrin ad Bisphenol A	1070:1099	The experimental conditions were studied for two endocrine disrupting compounds (Epichlorohydrin ad Bisphenol A) and at optimum conditions the adsorption capacity was 6.73 mgg-1 for epichlorohydrin and 7.01 mgg-1 for bisphenol A.
31923752	4	32	theme	Epichlorohydrin	1070:1084	arg1	compounds					1059:1067	two endocrine disrupting compounds	1034:1067	two endocrine disrupting compounds (Epichlorohydrin ad Bisphenol A)	1034:1100	The experimental conditions were studied for two endocrine disrupting compounds (Epichlorohydrin ad Bisphenol A) and at optimum conditions the adsorption capacity was 6.73 mgg-1 for epichlorohydrin and 7.01 mgg-1 for bisphenol A.
31923752	2	33	theme	dispersive	722:731	arg1	spectroscopy					739:750	energy dispersive X ray spectroscopy	715:750	energy dispersive X ray spectroscopy	715:750	The beads were characterized using spectroscopic techniques, such as Fourier transform infra-red spectroscopy for the determination of different functional groups, Scanning electron microscopy for surface morphology, X-ray diffraction for phase determination and energy dispersive X ray spectroscopy for elemental composition.
31923752	0	34	theme	compounds	121:129	arg1	microextraction					81:95	solid phase microextraction	69:95	solid phase microextraction of endocrine disrupting compounds from water samples	69:148	Eco-friendly alginate encapsulated magnetic graphene oxide beads for solid phase microextraction of endocrine disrupting compounds from water samples.
31923752	1	35	theme	liquid	403:408	arg1	chromatography					410:423	high performance liquid chromatography	386:423	high performance liquid chromatography with ultraviolet detector	386:449	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	4	36	theme	adsorption	1132:1141	arg1	6.73 mgg-1					1156:1165	6.73 mgg-1	1156:1165	6.73 mgg-1 for epichlorohydrin and 7.01 mgg-1 for bisphenol A	1156:1216	The experimental conditions were studied for two endocrine disrupting compounds (Epichlorohydrin ad Bisphenol A) and at optimum conditions the adsorption capacity was 6.73 mgg-1 for epichlorohydrin and 7.01 mgg-1 for bisphenol A.
31923752	4	36	theme	adsorption	1132:1141	arg1	capacity					1143:1150	the adsorption capacity	1128:1150	the adsorption capacity	1128:1150	The experimental conditions were studied for two endocrine disrupting compounds (Epichlorohydrin ad Bisphenol A) and at optimum conditions the adsorption capacity was 6.73 mgg-1 for epichlorohydrin and 7.01 mgg-1 for bisphenol A.
31923752	1	37	link	crosslinked	179:189	arg1	adsorbent					275:283	adsorbent	275:283	adsorbent	275:283	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	1	37	link	crosslinked	179:189	arg1	beads					237:241	crosslinked alginate encapsulated magnetic graphene oxide beads	179:241	crosslinked alginate encapsulated magnetic graphene oxide beads	179:241	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	0	38	theme	Eco-friendly	0:11	arg1	alginate					13:20	Eco-friendly alginate	0:20	Eco-friendly alginate	0:20	Eco-friendly alginate encapsulated magnetic graphene oxide beads for solid phase microextraction of endocrine disrupting compounds from water samples.
31923752	5	39	theme	pseudo-second	1299:1311	arg1	models					1353:1358	pseudo-second order kinetics and Langmuir equilibrium models	1299:1358	pseudo-second order kinetics and Langmuir equilibrium models	1299:1358	The kinetic and equilibrium studies revealed that the adsorption process follow pseudo-second order kinetics and Langmuir equilibrium models.
31923752	2	40	theme	spectroscopic	487:499	arg1	techniques					501:510	spectroscopic techniques	487:510	spectroscopic techniques	487:510	The beads were characterized using spectroscopic techniques, such as Fourier transform infra-red spectroscopy for the determination of different functional groups, Scanning electron microscopy for surface morphology, X-ray diffraction for phase determination and energy dispersive X ray spectroscopy for elemental composition.
31923752	2	40	theme	spectroscopic	487:499	arg1	Fourier					521:527	Fourier	521:527	Fourier transform infra-red spectroscopy for the determination of different functional groups, Scanning electron microscopy for surface morphology, X-ray diffraction for phase determination and energy dispersive X ray spectroscopy for elemental composition	521:776	The beads were characterized using spectroscopic techniques, such as Fourier transform infra-red spectroscopy for the determination of different functional groups, Scanning electron microscopy for surface morphology, X-ray diffraction for phase determination and energy dispersive X ray spectroscopy for elemental composition.
31923752	9	41	theme	toxic	1978:1982	arg1	compounds					1992:2000	toxic organic compounds	1978:2000	toxic organic compounds in water treatment	1978:2019	The magnetic graphene oxide encapsulated inside an alginate shows nontoxic green chemical with high extraction performance for toxic organic compounds in water treatment.
31923752	5	42	theme	kinetics	1319:1326	arg1	models					1353:1358	pseudo-second order kinetics and Langmuir equilibrium models	1299:1358	pseudo-second order kinetics and Langmuir equilibrium models	1299:1358	The kinetic and equilibrium studies revealed that the adsorption process follow pseudo-second order kinetics and Langmuir equilibrium models.
31923752	8	43	theme	spiked	1738:1743	arg1	samples					1745:1751	spiked samples	1738:1751	spiked samples	1738:1751	The proposed method was applied to spiked samples and the recovery values were 97.17 ± 3.13% for epichlorohydrin and 99.46 ± 1.39% for bisphenol A.
31923752	0	44	theme	graphene	44:51	arg1	beads					59:63	magnetic graphene oxide beads	35:63	magnetic graphene oxide beads	35:63	Eco-friendly alginate encapsulated magnetic graphene oxide beads for solid phase microextraction of endocrine disrupting compounds from water samples.
31923752	1	45	theme	compounds	333:341	arg1	microextraction					293:307	the microextraction	289:307	the microextraction of endocrine disrupting compounds from water samples	289:360	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	4	46	from	conditions	1117:1126	arg1	6.73 mgg-1					1156:1165	6.73 mgg-1	1156:1165	6.73 mgg-1 for epichlorohydrin and 7.01 mgg-1 for bisphenol A	1156:1216	The experimental conditions were studied for two endocrine disrupting compounds (Epichlorohydrin ad Bisphenol A) and at optimum conditions the adsorption capacity was 6.73 mgg-1 for epichlorohydrin and 7.01 mgg-1 for bisphenol A.
31923752	4	46	from	conditions	1117:1126	arg1	capacity					1143:1150	the adsorption capacity	1128:1150	the adsorption capacity	1128:1150	The experimental conditions were studied for two endocrine disrupting compounds (Epichlorohydrin ad Bisphenol A) and at optimum conditions the adsorption capacity was 6.73 mgg-1 for epichlorohydrin and 7.01 mgg-1 for bisphenol A.
31923752	9	47	theme	high	1946:1949	arg1	performance					1962:1972	high extraction performance	1946:1972	high extraction performance for toxic organic compounds in water treatment	1946:2019	The magnetic graphene oxide encapsulated inside an alginate shows nontoxic green chemical with high extraction performance for toxic organic compounds in water treatment.
31923752	1	48	theme	water	348:352	arg1	samples					354:360	water samples	348:360	water samples	348:360	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	8	49	theme	recovery	1761:1768	arg1	%					1794:1794	97.17 ± 3.13%	1782:1794	97.17 ± 3.13% for epichlorohydrin	1782:1814	The proposed method was applied to spiked samples and the recovery values were 97.17 ± 3.13% for epichlorohydrin and 99.46 ± 1.39% for bisphenol A.
31923752	8	49	theme	recovery	1761:1768	arg1	values					1770:1775	the recovery values	1757:1775	the recovery values	1757:1775	The proposed method was applied to spiked samples and the recovery values were 97.17 ± 3.13% for epichlorohydrin and 99.46 ± 1.39% for bisphenol A.
31923752	7	50	theme	compounds	1664:1672	arg1	microextraction					1596:1610	microextraction	1596:1610	microextraction	1596:1610	Different solvents used for microextraction and maximum extraction of both endocrine disrupting compounds were obtained with methanol.
31923752	7	50	theme	compounds	1664:1672	arg1	extraction					1624:1633	maximum extraction	1616:1633	maximum extraction	1616:1633	Different solvents used for microextraction and maximum extraction of both endocrine disrupting compounds were obtained with methanol.
31923752	6	51	theme	A.	1456:1457	arg1	Limit					1459:1463	bisphenol A. Limit	1446:1463	bisphenol A. Limit of detection	1446:1476	Analytical parameters were calculated for the microextraction of epichlorohydrin and bisphenol A. Limit of detection was 8.25 ngL-1 and 13.99 ngL-1 (n = 4) for epichlorohydrin and bisphenol A, respectively.
31923752	5	52	theme	Langmuir	1332:1339	arg1	equilibrium					1341:1351	Langmuir equilibrium	1332:1351	Langmuir equilibrium	1332:1351	The kinetic and equilibrium studies revealed that the adsorption process follow pseudo-second order kinetics and Langmuir equilibrium models.
31923752	7	53	theme	endocrine	1643:1651	arg1	compounds					1664:1672	both endocrine disrupting compounds	1638:1672	both endocrine disrupting compounds	1638:1672	Different solvents used for microextraction and maximum extraction of both endocrine disrupting compounds were obtained with methanol.
31923752	3	54	theme	functional	824:833	arg1	alginate					845:852	alginate	845:852	alginate	845:852	The results revealed that beads surface have functional groups of alginate and graphene oxide which are involved in π-π, n-πinteractions and hydrogen bonding for the bisphenol A and epichlorohydrin adsorption.
31923752	3	54	theme	functional	824:833	arg1	oxide					867:871	graphene oxide	858:871	graphene oxide	858:871	The results revealed that beads surface have functional groups of alginate and graphene oxide which are involved in π-π, n-πinteractions and hydrogen bonding for the bisphenol A and epichlorohydrin adsorption.
31923752	3	54	theme	functional	824:833	arg1	groups					835:840	functional groups	824:840	functional groups of alginate and graphene oxide which are involved in π-π, n-πinteractions and hydrogen bonding for the bisphenol A and epichlorohydrin adsorption	824:986	The results revealed that beads surface have functional groups of alginate and graphene oxide which are involved in π-π, n-πinteractions and hydrogen bonding for the bisphenol A and epichlorohydrin adsorption.
31923752	1	55	theme	crosslinked	179:189	arg1	adsorbent					275:283	adsorbent	275:283	adsorbent	275:283	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	1	55	theme	crosslinked	179:189	arg1	beads					237:241	crosslinked alginate encapsulated magnetic graphene oxide beads	179:241	crosslinked alginate encapsulated magnetic graphene oxide beads	179:241	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	7	56	theme	maximum	1616:1622	arg1	extraction					1624:1633	maximum extraction	1616:1633	maximum extraction	1616:1633	Different solvents used for microextraction and maximum extraction of both endocrine disrupting compounds were obtained with methanol.
31923752	9	57	theme	magnetic	1855:1862	arg1	oxide					1873:1877	The magnetic graphene oxide	1851:1877	The magnetic graphene oxide encapsulated inside an alginate	1851:1909	The magnetic graphene oxide encapsulated inside an alginate shows nontoxic green chemical with high extraction performance for toxic organic compounds in water treatment.
31923752	1	58	from	microextraction	293:307	arg1	samples					354:360	water samples	348:360	water samples	348:360	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	1	59	theme	encapsulated	200:211	arg1	adsorbent					275:283	adsorbent	275:283	adsorbent	275:283	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	1	59	theme	encapsulated	200:211	arg1	beads					237:241	crosslinked alginate encapsulated magnetic graphene oxide beads	179:241	crosslinked alginate encapsulated magnetic graphene oxide beads	179:241	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	2	60	theme	phase	691:695	arg1	determination					697:709	phase determination	691:709	phase determination	691:709	The beads were characterized using spectroscopic techniques, such as Fourier transform infra-red spectroscopy for the determination of different functional groups, Scanning electron microscopy for surface morphology, X-ray diffraction for phase determination and energy dispersive X ray spectroscopy for elemental composition.
31923752	1	61	theme	graphene	222:229	arg1	adsorbent					275:283	adsorbent	275:283	adsorbent	275:283	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	1	61	theme	graphene	222:229	arg1	beads					237:241	crosslinked alginate encapsulated magnetic graphene oxide beads	179:241	crosslinked alginate encapsulated magnetic graphene oxide beads	179:241	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	2	62	theme	Scanning	616:623	arg1	microscopy					634:643	Scanning electron microscopy	616:643	Scanning electron microscopy for surface morphology	616:666	The beads were characterized using spectroscopic techniques, such as Fourier transform infra-red spectroscopy for the determination of different functional groups, Scanning electron microscopy for surface morphology, X-ray diffraction for phase determination and energy dispersive X ray spectroscopy for elemental composition.
31923752	1	63	theme	ultraviolet	430:440	arg1	detector					442:449	ultraviolet detector	430:449	ultraviolet detector	430:449	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	2	64	theme	groups	608:613	arg1	determination					570:582	the determination	566:582	the determination of different functional groups	566:613	The beads were characterized using spectroscopic techniques, such as Fourier transform infra-red spectroscopy for the determination of different functional groups, Scanning electron microscopy for surface morphology, X-ray diffraction for phase determination and energy dispersive X ray spectroscopy for elemental composition.
31923752	2	65	theme	surface	649:655	arg1	morphology					657:666	surface morphology	649:666	surface morphology	649:666	The beads were characterized using spectroscopic techniques, such as Fourier transform infra-red spectroscopy for the determination of different functional groups, Scanning electron microscopy for surface morphology, X-ray diffraction for phase determination and energy dispersive X ray spectroscopy for elemental composition.
31923752	6	66	theme	Analytical	1361:1370	arg1	parameters					1372:1381	Analytical parameters	1361:1381	Analytical parameters	1361:1381	Analytical parameters were calculated for the microextraction of epichlorohydrin and bisphenol A. Limit of detection was 8.25 ngL-1 and 13.99 ngL-1 (n = 4) for epichlorohydrin and bisphenol A, respectively.
31923752	3	67	theme	oxide	867:871	arg1	alginate					845:852	alginate	845:852	alginate	845:852	The results revealed that beads surface have functional groups of alginate and graphene oxide which are involved in π-π, n-πinteractions and hydrogen bonding for the bisphenol A and epichlorohydrin adsorption.
31923752	3	67	theme	oxide	867:871	arg1	oxide					867:871	graphene oxide	858:871	graphene oxide	858:871	The results revealed that beads surface have functional groups of alginate and graphene oxide which are involved in π-π, n-πinteractions and hydrogen bonding for the bisphenol A and epichlorohydrin adsorption.
31923752	3	67	theme	oxide	867:871	arg1	groups					835:840	functional groups	824:840	functional groups of alginate and graphene oxide which are involved in π-π, n-πinteractions and hydrogen bonding for the bisphenol A and epichlorohydrin adsorption	824:986	The results revealed that beads surface have functional groups of alginate and graphene oxide which are involved in π-π, n-πinteractions and hydrogen bonding for the bisphenol A and epichlorohydrin adsorption.
31923752	1	68	with	chromatography	410:423	arg1	detector					442:449	ultraviolet detector	430:449	ultraviolet detector	430:449	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	0	69	theme	phase	75:79	arg1	microextraction					81:95	solid phase microextraction	69:95	solid phase microextraction of endocrine disrupting compounds from water samples	69:148	Eco-friendly alginate encapsulated magnetic graphene oxide beads for solid phase microextraction of endocrine disrupting compounds from water samples.
31923752	4	70	theme	ad	1086:1087	arg1	A					1099:1099	Epichlorohydrin ad Bisphenol A	1070:1099	Epichlorohydrin ad Bisphenol A	1070:1099	The experimental conditions were studied for two endocrine disrupting compounds (Epichlorohydrin ad Bisphenol A) and at optimum conditions the adsorption capacity was 6.73 mgg-1 for epichlorohydrin and 7.01 mgg-1 for bisphenol A.
31923752	4	70	theme	ad	1086:1087	arg1	compounds					1059:1067	two endocrine disrupting compounds	1034:1067	two endocrine disrupting compounds (Epichlorohydrin ad Bisphenol A)	1034:1100	The experimental conditions were studied for two endocrine disrupting compounds (Epichlorohydrin ad Bisphenol A) and at optimum conditions the adsorption capacity was 6.73 mgg-1 for epichlorohydrin and 7.01 mgg-1 for bisphenol A.
31923752	2	71	theme	different	587:595	arg1	groups					608:613	different functional groups	587:613	different functional groups	587:613	The beads were characterized using spectroscopic techniques, such as Fourier transform infra-red spectroscopy for the determination of different functional groups, Scanning electron microscopy for surface morphology, X-ray diffraction for phase determination and energy dispersive X ray spectroscopy for elemental composition.
31923752	6	72	theme	bisphenol	1541:1549	arg1	A					1551:1551	bisphenol A	1541:1551	bisphenol A	1541:1551	Analytical parameters were calculated for the microextraction of epichlorohydrin and bisphenol A. Limit of detection was 8.25 ngL-1 and 13.99 ngL-1 (n = 4) for epichlorohydrin and bisphenol A, respectively.
31923752	1	73	theme	method	167:172	arg1	iron					174:177	the proposed method iron	154:177	the proposed method iron	154:177	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	2	74	theme	infra-red	539:547	arg1	spectroscopy					549:560	infra-red spectroscopy	539:560	infra-red spectroscopy for the determination of different functional groups	539:613	The beads were characterized using spectroscopic techniques, such as Fourier transform infra-red spectroscopy for the determination of different functional groups, Scanning electron microscopy for surface morphology, X-ray diffraction for phase determination and energy dispersive X ray spectroscopy for elemental composition.
31923752	0	75	theme	disrupting	110:119	arg1	compounds					121:129	endocrine disrupting compounds	100:129	endocrine disrupting compounds from water samples	100:148	Eco-friendly alginate encapsulated magnetic graphene oxide beads for solid phase microextraction of endocrine disrupting compounds from water samples.
31923752	3	76	theme	epichlorohydrin	961:975	arg1	adsorption					977:986	epichlorohydrin adsorption	961:986	epichlorohydrin adsorption	961:986	The results revealed that beads surface have functional groups of alginate and graphene oxide which are involved in π-π, n-πinteractions and hydrogen bonding for the bisphenol A and epichlorohydrin adsorption.
31923752	4	77	theme	disrupting	1048:1057	arg1	A					1099:1099	Epichlorohydrin ad Bisphenol A	1070:1099	Epichlorohydrin ad Bisphenol A	1070:1099	The experimental conditions were studied for two endocrine disrupting compounds (Epichlorohydrin ad Bisphenol A) and at optimum conditions the adsorption capacity was 6.73 mgg-1 for epichlorohydrin and 7.01 mgg-1 for bisphenol A.
31923752	4	77	theme	disrupting	1048:1057	arg1	compounds					1059:1067	two endocrine disrupting compounds	1034:1067	two endocrine disrupting compounds (Epichlorohydrin ad Bisphenol A)	1034:1100	The experimental conditions were studied for two endocrine disrupting compounds (Epichlorohydrin ad Bisphenol A) and at optimum conditions the adsorption capacity was 6.73 mgg-1 for epichlorohydrin and 7.01 mgg-1 for bisphenol A.
31923752	8	78	theme	proposed	1707:1714	arg1	method					1716:1721	The proposed method	1703:1721	The proposed method	1703:1721	The proposed method was applied to spiked samples and the recovery values were 97.17 ± 3.13% for epichlorohydrin and 99.46 ± 1.39% for bisphenol A.
31923752	2	79	theme	X	733:733	arg1	spectroscopy					739:750	energy dispersive X ray spectroscopy	715:750	energy dispersive X ray spectroscopy	715:750	The beads were characterized using spectroscopic techniques, such as Fourier transform infra-red spectroscopy for the determination of different functional groups, Scanning electron microscopy for surface morphology, X-ray diffraction for phase determination and energy dispersive X ray spectroscopy for elemental composition.
31923752	1	80	theme	performance	391:401	arg1	chromatography					410:423	high performance liquid chromatography	386:423	high performance liquid chromatography with ultraviolet detector	386:449	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	3	81	dep	A	955:955	arg1	the					941:943	the	941:943	the	941:943	The results revealed that beads surface have functional groups of alginate and graphene oxide which are involved in π-π, n-πinteractions and hydrogen bonding for the bisphenol A and epichlorohydrin adsorption.
31923752	9	82	theme	organic	1984:1990	arg1	compounds					1992:2000	toxic organic compounds	1978:2000	toxic organic compounds in water treatment	1978:2019	The magnetic graphene oxide encapsulated inside an alginate shows nontoxic green chemical with high extraction performance for toxic organic compounds in water treatment.
31923752	9	83	theme	water	2005:2009	arg1	treatment					2011:2019	water treatment	2005:2019	water treatment	2005:2019	The magnetic graphene oxide encapsulated inside an alginate shows nontoxic green chemical with high extraction performance for toxic organic compounds in water treatment.
31923752	2	84	theme	elemental	756:764	arg1	composition					766:776	elemental composition	756:776	elemental composition	756:776	The beads were characterized using spectroscopic techniques, such as Fourier transform infra-red spectroscopy for the determination of different functional groups, Scanning electron microscopy for surface morphology, X-ray diffraction for phase determination and energy dispersive X ray spectroscopy for elemental composition.
31923752	0	85	theme	magnetic	35:42	arg1	beads					59:63	magnetic graphene oxide beads	35:63	magnetic graphene oxide beads	35:63	Eco-friendly alginate encapsulated magnetic graphene oxide beads for solid phase microextraction of endocrine disrupting compounds from water samples.
31923752	5	86	theme	order	1313:1317	arg1	kinetics					1319:1326	order kinetics	1313:1326	order kinetics	1313:1326	The kinetic and equilibrium studies revealed that the adsorption process follow pseudo-second order kinetics and Langmuir equilibrium models.
31923752	0	87	theme	oxide	53:57	arg1	beads					59:63	magnetic graphene oxide beads	35:63	magnetic graphene oxide beads	35:63	Eco-friendly alginate encapsulated magnetic graphene oxide beads for solid phase microextraction of endocrine disrupting compounds from water samples.
31923752	1	88	theme	disrupting	322:331	arg1	compounds					333:341	endocrine disrupting compounds	312:341	endocrine disrupting compounds from water samples	312:360	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	9	89	from	compounds	1992:2000	arg1	treatment					2011:2019	water treatment	2005:2019	water treatment	2005:2019	The magnetic graphene oxide encapsulated inside an alginate shows nontoxic green chemical with high extraction performance for toxic organic compounds in water treatment.
31923752	5	90	theme	adsorption	1273:1282	arg1	process					1284:1290	the adsorption process	1269:1290	the adsorption process	1269:1290	The kinetic and equilibrium studies revealed that the adsorption process follow pseudo-second order kinetics and Langmuir equilibrium models.
31923752	5	91	theme	kinetic	1223:1229	arg1	studies					1247:1253	The kinetic and equilibrium studies	1219:1253	studies	1247:1253	The kinetic and equilibrium studies revealed that the adsorption process follow pseudo-second order kinetics and Langmuir equilibrium models.
31923752	4	92	theme	optimum	1109:1115	arg1	conditions					1117:1126	optimum conditions	1109:1126	optimum conditions	1109:1126	The experimental conditions were studied for two endocrine disrupting compounds (Epichlorohydrin ad Bisphenol A) and at optimum conditions the adsorption capacity was 6.73 mgg-1 for epichlorohydrin and 7.01 mgg-1 for bisphenol A.
31923752	9	93	with	chemical	1932:1939	arg1	performance					1962:1972	high extraction performance	1946:1972	high extraction performance for toxic organic compounds in water treatment	1946:2019	The magnetic graphene oxide encapsulated inside an alginate shows nontoxic green chemical with high extraction performance for toxic organic compounds in water treatment.
31923752	8	94	theme	bisphenol	1838:1846	arg1	A					1848:1848	bisphenol A	1838:1848	bisphenol A	1838:1848	The proposed method was applied to spiked samples and the recovery values were 97.17 ± 3.13% for epichlorohydrin and 99.46 ± 1.39% for bisphenol A.
31923752	6	95	theme	bisphenol	1446:1454	arg1	Limit					1459:1463	bisphenol A. Limit	1446:1463	bisphenol A. Limit of detection	1446:1476	Analytical parameters were calculated for the microextraction of epichlorohydrin and bisphenol A. Limit of detection was 8.25 ngL-1 and 13.99 ngL-1 (n = 4) for epichlorohydrin and bisphenol A, respectively.
31923752	1	96	used	used	264:267	arg2	adsorbent					275:283	adsorbent	275:283	adsorbent	275:283	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	1	96	used	used	264:267	arg2	beads					237:241	crosslinked alginate encapsulated magnetic graphene oxide beads	179:241	crosslinked alginate encapsulated magnetic graphene oxide beads	179:241	In the proposed method iron crosslinked alginate encapsulated magnetic graphene oxide beads were synthesized and used as an adsorbent for the microextraction of endocrine disrupting compounds from water samples and further analyzed by high performance liquid chromatography with ultraviolet detector.
31923752	4	97	theme	bisphenol	1206:1214	arg1	A					1216:1216	bisphenol A	1206:1216	bisphenol A	1206:1216	The experimental conditions were studied for two endocrine disrupting compounds (Epichlorohydrin ad Bisphenol A) and at optimum conditions the adsorption capacity was 6.73 mgg-1 for epichlorohydrin and 7.01 mgg-1 for bisphenol A.
31923752	0	98	from	samples	142:148	arg1	microextraction					81:95	solid phase microextraction	69:95	solid phase microextraction of endocrine disrupting compounds from water samples	69:148	Eco-friendly alginate encapsulated magnetic graphene oxide beads for solid phase microextraction of endocrine disrupting compounds from water samples.
31923752	0	98	from	samples	142:148	arg1	compounds					121:129	endocrine disrupting compounds	100:129	endocrine disrupting compounds from water samples	100:148	Eco-friendly alginate encapsulated magnetic graphene oxide beads for solid phase microextraction of endocrine disrupting compounds from water samples.
32152415	0	0	theme	HIV	112:114	arg1	infection					116:124	HIV infection	112:124	HIV infection	112:124	Sialylation and fucosylation modulate inflammasome-activating eIF2 Signaling and microbial translocation during HIV infection.
32152415	6	1	theme	HIV	1409:1411	arg1	infection					1413:1421	HIV infection	1409:1421	HIV infection	1409:1421	Our study thus provides the first proof-of-concept evidence that a previously unappreciated factor, gut glycosylation, is a force that may impact the vicious cycle between HIV infection, microbial translocation, and chronic inflammation.
32152415	3	2	theme	sigmoid	544:550	arg1	colon					552:556	sigmoid colon	544:556	sigmoid colon	544:556	We examined terminal ileum, right colon, and sigmoid colon biopsies from HIV-infected virally-suppressed individuals and found that gut glycomic patterns are associated with distinct microbial compositions and differential levels of chronic inflammation and HIV persistence.
32152415	6	3	theme	proof-of-concept	1271:1286	arg1	evidence					1288:1295	the first proof-of-concept evidence that a previously unappreciated factor, gut glycosylation, is a force that may impact the vicious cycle between HIV infection, microbial translocation, and chronic inflammation	1261:1472	the first proof-of-concept evidence that a previously unappreciated factor, gut glycosylation, is a force that may impact the vicious cycle between HIV infection, microbial translocation, and chronic inflammation	1261:1472	Our study thus provides the first proof-of-concept evidence that a previously unappreciated factor, gut glycosylation, is a force that may impact the vicious cycle between HIV infection, microbial translocation, and chronic inflammation.
32152415	4	4	theme	species	987:993	arg1	abundance					947:955	higher abundance	940:955	higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA	940:1136	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	3	5	theme	colon	552:556	arg1	biopsies					558:565	terminal ileum, right colon, and sigmoid colon biopsies	511:565	terminal ileum, right colon, and sigmoid colon biopsies from HIV-infected virally-suppressed individuals	511:614	We examined terminal ileum, right colon, and sigmoid colon biopsies from HIV-infected virally-suppressed individuals and found that gut glycomic patterns are associated with distinct microbial compositions and differential levels of chronic inflammation and HIV persistence.
32152415	3	6	from	individuals	604:614	arg1	biopsies					558:565	terminal ileum, right colon, and sigmoid colon biopsies	511:565	terminal ileum, right colon, and sigmoid colon biopsies from HIV-infected virally-suppressed individuals	511:614	We examined terminal ileum, right colon, and sigmoid colon biopsies from HIV-infected virally-suppressed individuals and found that gut glycomic patterns are associated with distinct microbial compositions and differential levels of chronic inflammation and HIV persistence.
32152415	6	7	theme	vicious	1387:1393	arg1	cycle					1395:1399	the vicious cycle	1383:1399	the vicious cycle between HIV infection, microbial translocation, and chronic inflammation	1383:1472	Our study thus provides the first proof-of-concept evidence that a previously unappreciated factor, gut glycosylation, is a force that may impact the vicious cycle between HIV infection, microbial translocation, and chronic inflammation.
32152415	2	8	theme	gut	307:309	arg1	glycosylation					311:323	gut glycosylation	307:323	gut glycosylation	307:323	Nevertheless, it is unclear how gut glycosylation contributes to the HIV-associated microbial translocation and inflammation that persist despite viral suppression and contribute to the development of several comorbidities.
32152415	2	9	dep	translocation	369:381	arg1	the					340:342	the	340:342	the	340:342	Nevertheless, it is unclear how gut glycosylation contributes to the HIV-associated microbial translocation and inflammation that persist despite viral suppression and contribute to the development of several comorbidities.
32152415	1	10	theme	key	185:187	arg1	force					189:193	a key force	183:193	a key force in maintaining the homeostatic relationship between the gut and its microbiota	183:272	An emerging paradigm suggests that gut glycosylation is a key force in maintaining the homeostatic relationship between the gut and its microbiota.
32152415	1	10	theme	key	185:187	arg1	glycosylation					166:178	gut glycosylation	162:178	gut glycosylation	162:178	An emerging paradigm suggests that gut glycosylation is a key force in maintaining the homeostatic relationship between the gut and its microbiota.
32152415	4	11	gly	hypo-sialylated	825:839	arg1	glycans					851:857	the pro-inflammatory hypo-sialylated T-antigen glycans	804:857	the pro-inflammatory hypo-sialylated T-antigen glycans	804:857	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	5	12	theme	inflammasome-mediating	1190:1211	arg1	pathway					1228:1234	the inflammasome-mediating eIF2 signaling pathway	1186:1234	the inflammasome-mediating eIF2 signaling pathway	1186:1234	These findings are linked to the activation of the inflammasome-mediating eIF2 signaling pathway.
32152415	1	13	theme	gut	162:164	arg1	force					189:193	a key force	183:193	a key force in maintaining the homeostatic relationship between the gut and its microbiota	183:272	An emerging paradigm suggests that gut glycosylation is a key force in maintaining the homeostatic relationship between the gut and its microbiota.
32152415	1	13	theme	gut	162:164	arg1	glycosylation					166:178	gut glycosylation	162:178	gut glycosylation	162:178	An emerging paradigm suggests that gut glycosylation is a key force in maintaining the homeostatic relationship between the gut and its microbiota.
32152415	4	14	theme	levels	1068:1073	arg1	abundance					947:955	higher abundance	940:955	higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA	940:1136	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	4	15	theme	pro-inflammatory	808:823	arg1	glycans					851:857	the pro-inflammatory hypo-sialylated T-antigen glycans	804:857	the pro-inflammatory hypo-sialylated T-antigen glycans	804:857	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	4	16	theme	hypo-sialylated	825:839	arg1	glycans					851:857	the pro-inflammatory hypo-sialylated T-antigen glycans	804:857	the pro-inflammatory hypo-sialylated T-antigen glycans	804:857	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	3	17	theme	HIV-infected	572:583	arg1	individuals					604:614	HIV-infected virally-suppressed individuals	572:614	HIV-infected virally-suppressed individuals	572:614	We examined terminal ileum, right colon, and sigmoid colon biopsies from HIV-infected virally-suppressed individuals and found that gut glycomic patterns are associated with distinct microbial compositions and differential levels of chronic inflammation and HIV persistence.
32152415	4	18	theme	higher	1061:1066	arg1	levels					1068:1073	higher levels	1061:1073	higher levels of inflammation	1061:1089	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	5	19	theme	signaling	1218:1226	arg1	pathway					1228:1234	the inflammasome-mediating eIF2 signaling pathway	1186:1234	the inflammasome-mediating eIF2 signaling pathway	1186:1234	These findings are linked to the activation of the inflammasome-mediating eIF2 signaling pathway.
32152415	4	20	theme	levels	1103:1108	arg1	abundance					947:955	higher abundance	940:955	higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA	940:1136	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	2	21	theme	HIV-associated	344:357	arg1	translocation					369:381	HIV-associated microbial translocation	344:381	HIV-associated microbial translocation	344:381	Nevertheless, it is unclear how gut glycosylation contributes to the HIV-associated microbial translocation and inflammation that persist despite viral suppression and contribute to the development of several comorbidities.
32152415	4	22	theme	DNA	1134:1136	arg1	microbiome					1049:1058	a less diverse microbiome	1034:1058	a less diverse microbiome	1034:1058	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	4	22	theme	DNA	1134:1136	arg1	levels					1068:1073	higher levels	1061:1073	higher levels of inflammation	1061:1089	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	4	22	theme	DNA	1134:1136	arg1	levels					1103:1108	higher levels	1096:1108	higher levels of ileum-associated HIV DNA	1096:1136	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	4	22	theme	DNA	1134:1136	arg1	species					987:993	glycan-degrading microbial species	960:993	glycan-degrading microbial species (in particular, Bacteroides vulgatus)	960:1031	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	5	23	theme	pathway	1228:1234	arg1	activation					1172:1181	the activation	1168:1181	the activation of the inflammasome-mediating eIF2 signaling pathway	1168:1234	These findings are linked to the activation of the inflammasome-mediating eIF2 signaling pathway.
32152415	4	24	theme	microbial	977:985	arg1	species					987:993	glycan-degrading microbial species	960:993	glycan-degrading microbial species (in particular, Bacteroides vulgatus)	960:1031	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	3	25	theme	gut	631:633	arg1	patterns					644:651	gut glycomic patterns	631:651	gut glycomic patterns	631:651	We examined terminal ileum, right colon, and sigmoid colon biopsies from HIV-infected virally-suppressed individuals and found that gut glycomic patterns are associated with distinct microbial compositions and differential levels of chronic inflammation and HIV persistence.
32152415	5	26	theme	eIF2	1213:1216	arg1	pathway					1228:1234	the inflammasome-mediating eIF2 signaling pathway	1186:1234	the inflammasome-mediating eIF2 signaling pathway	1186:1234	These findings are linked to the activation of the inflammasome-mediating eIF2 signaling pathway.
32152415	4	27	theme	HIV	1130:1132	arg1	DNA					1134:1136	ileum-associated HIV DNA	1113:1136	ileum-associated HIV DNA	1113:1136	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	4	28	theme	glycan-degrading	960:975	arg1	species					987:993	glycan-degrading microbial species	960:993	glycan-degrading microbial species (in particular, Bacteroides vulgatus)	960:1031	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	4	29	theme	ileum-associated	1113:1128	arg1	DNA					1134:1136	ileum-associated HIV DNA	1113:1136	ileum-associated HIV DNA	1113:1136	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	3	30	theme	glycomic	635:642	arg1	patterns					644:651	gut glycomic patterns	631:651	gut glycomic patterns	631:651	We examined terminal ileum, right colon, and sigmoid colon biopsies from HIV-infected virally-suppressed individuals and found that gut glycomic patterns are associated with distinct microbial compositions and differential levels of chronic inflammation and HIV persistence.
32152415	2	31	theme	viral	421:425	arg1	suppression					427:437	viral suppression	421:437	viral suppression	421:437	Nevertheless, it is unclear how gut glycosylation contributes to the HIV-associated microbial translocation and inflammation that persist despite viral suppression and contribute to the development of several comorbidities.
32152415	2	32	theme	comorbidities	484:496	arg1	development					461:471	the development	457:471	the development of several comorbidities	457:496	Nevertheless, it is unclear how gut glycosylation contributes to the HIV-associated microbial translocation and inflammation that persist despite viral suppression and contribute to the development of several comorbidities.
32152415	4	33	theme	glycans	911:917	arg1	levels					794:799	high levels	789:799	high levels of the pro-inflammatory hypo-sialylated T-antigen glycans	789:857	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	4	33	theme	glycans	911:917	arg1	levels					867:872	low levels	863:872	low levels of the anti-inflammatory fucosylated glycans	863:917	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	6	34	theme	chronic	1453:1459	arg1	inflammation					1461:1472	chronic inflammation	1453:1472	chronic inflammation	1453:1472	Our study thus provides the first proof-of-concept evidence that a previously unappreciated factor, gut glycosylation, is a force that may impact the vicious cycle between HIV infection, microbial translocation, and chronic inflammation.
32152415	4	35	theme	glycans	851:857	arg1	levels					794:799	high levels	789:799	high levels of the pro-inflammatory hypo-sialylated T-antigen glycans	789:857	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	4	35	theme	glycans	851:857	arg1	levels					867:872	low levels	863:872	low levels of the anti-inflammatory fucosylated glycans	863:917	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	3	36	theme	ileum	520:524	arg1	biopsies					558:565	terminal ileum, right colon, and sigmoid colon biopsies	511:565	terminal ileum, right colon, and sigmoid colon biopsies from HIV-infected virally-suppressed individuals	511:614	We examined terminal ileum, right colon, and sigmoid colon biopsies from HIV-infected virally-suppressed individuals and found that gut glycomic patterns are associated with distinct microbial compositions and differential levels of chronic inflammation and HIV persistence.
32152415	2	37	theme	several	476:482	arg1	comorbidities					484:496	several comorbidities	476:496	several comorbidities	476:496	Nevertheless, it is unclear how gut glycosylation contributes to the HIV-associated microbial translocation and inflammation that persist despite viral suppression and contribute to the development of several comorbidities.
32152415	1	38	theme	emerging	130:137	arg1	paradigm					139:146	An emerging paradigm	127:146	An emerging paradigm	127:146	An emerging paradigm suggests that gut glycosylation is a key force in maintaining the homeostatic relationship between the gut and its microbiota.
32152415	6	39	theme	first	1265:1269	arg1	evidence					1288:1295	the first proof-of-concept evidence that a previously unappreciated factor, gut glycosylation, is a force that may impact the vicious cycle between HIV infection, microbial translocation, and chronic inflammation	1261:1472	the first proof-of-concept evidence that a previously unappreciated factor, gut glycosylation, is a force that may impact the vicious cycle between HIV infection, microbial translocation, and chronic inflammation	1261:1472	Our study thus provides the first proof-of-concept evidence that a previously unappreciated factor, gut glycosylation, is a force that may impact the vicious cycle between HIV infection, microbial translocation, and chronic inflammation.
32152415	0	40	theme	eIF2	62:65	arg1	Signaling					67:75	inflammasome-activating eIF2 Signaling	38:75	inflammasome-activating eIF2 Signaling	38:75	Sialylation and fucosylation modulate inflammasome-activating eIF2 Signaling and microbial translocation during HIV infection.
32152415	4	41	theme	high	789:792	arg1	levels					794:799	high levels	789:799	high levels of the pro-inflammatory hypo-sialylated T-antigen glycans	789:857	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	4	42	theme	diverse	1041:1047	arg1	microbiome					1049:1058	a less diverse microbiome	1034:1058	a less diverse microbiome	1034:1058	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	3	43	theme	chronic	732:738	arg1	inflammation					740:751	chronic inflammation	732:751	chronic inflammation	732:751	We examined terminal ileum, right colon, and sigmoid colon biopsies from HIV-infected virally-suppressed individuals and found that gut glycomic patterns are associated with distinct microbial compositions and differential levels of chronic inflammation and HIV persistence.
32152415	6	44	theme	unappreciated	1315:1327	arg1	force					1361:1365	a force	1359:1365	a force that may impact the vicious cycle between HIV infection, microbial translocation, and chronic inflammation	1359:1472	Our study thus provides the first proof-of-concept evidence that a previously unappreciated factor, gut glycosylation, is a force that may impact the vicious cycle between HIV infection, microbial translocation, and chronic inflammation.
32152415	6	44	theme	unappreciated	1315:1327	arg1	factor					1329:1334	a previously unappreciated factor	1302:1334	a previously unappreciated factor	1302:1334	Our study thus provides the first proof-of-concept evidence that a previously unappreciated factor, gut glycosylation, is a force that may impact the vicious cycle between HIV infection, microbial translocation, and chronic inflammation.
32152415	6	44	theme	unappreciated	1315:1327	arg1	glycosylation					1341:1353	gut glycosylation	1337:1353	gut glycosylation	1337:1353	Our study thus provides the first proof-of-concept evidence that a previously unappreciated factor, gut glycosylation, is a force that may impact the vicious cycle between HIV infection, microbial translocation, and chronic inflammation.
32152415	0	45	theme	inflammasome-activating	38:60	arg1	Signaling					67:75	inflammasome-activating eIF2 Signaling	38:75	inflammasome-activating eIF2 Signaling	38:75	Sialylation and fucosylation modulate inflammasome-activating eIF2 Signaling and microbial translocation during HIV infection.
32152415	4	46	theme	higher	1096:1101	arg1	levels					1103:1108	higher levels	1096:1108	higher levels of ileum-associated HIV DNA	1096:1136	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	1	47	dep	gut	251:253	arg1	the					247:249	the	247:249	the	247:249	An emerging paradigm suggests that gut glycosylation is a key force in maintaining the homeostatic relationship between the gut and its microbiota.
32152415	3	48	theme	terminal	511:518	arg1	ileum					520:524	terminal ileum	511:524	terminal ileum	511:524	We examined terminal ileum, right colon, and sigmoid colon biopsies from HIV-infected virally-suppressed individuals and found that gut glycomic patterns are associated with distinct microbial compositions and differential levels of chronic inflammation and HIV persistence.
32152415	4	49	gly	fucosylated	899:909	arg1	glycans					911:917	the anti-inflammatory fucosylated glycans	877:917	the anti-inflammatory fucosylated glycans	877:917	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	6	50	theme	microbial	1424:1432	arg1	translocation					1434:1446	microbial translocation	1424:1446	microbial translocation	1424:1446	Our study thus provides the first proof-of-concept evidence that a previously unappreciated factor, gut glycosylation, is a force that may impact the vicious cycle between HIV infection, microbial translocation, and chronic inflammation.
32152415	4	51	theme	higher	940:945	arg1	abundance					947:955	higher abundance	940:955	higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA	940:1136	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	4	52	dep	species	987:993	arg1	vulgatus					1023:1030	Bacteroides vulgatus	1011:1030	Bacteroides vulgatus	1011:1030	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	3	53	theme	HIV	757:759	arg1	persistence					761:771	HIV persistence	757:771	HIV persistence	757:771	We examined terminal ileum, right colon, and sigmoid colon biopsies from HIV-infected virally-suppressed individuals and found that gut glycomic patterns are associated with distinct microbial compositions and differential levels of chronic inflammation and HIV persistence.
32152415	3	54	theme	differential	709:720	arg1	levels					722:727	differential levels	709:727	differential levels of chronic inflammation and HIV persistence	709:771	We examined terminal ileum, right colon, and sigmoid colon biopsies from HIV-infected virally-suppressed individuals and found that gut glycomic patterns are associated with distinct microbial compositions and differential levels of chronic inflammation and HIV persistence.
32152415	4	55	theme	fucosylated	899:909	arg1	glycans					911:917	the anti-inflammatory fucosylated glycans	877:917	the anti-inflammatory fucosylated glycans	877:917	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	3	56	theme	persistence	761:771	arg1	compositions					692:703	distinct microbial compositions	673:703	distinct microbial compositions	673:703	We examined terminal ileum, right colon, and sigmoid colon biopsies from HIV-infected virally-suppressed individuals and found that gut glycomic patterns are associated with distinct microbial compositions and differential levels of chronic inflammation and HIV persistence.
32152415	3	56	theme	persistence	761:771	arg1	levels					722:727	differential levels	709:727	differential levels of chronic inflammation and HIV persistence	709:771	We examined terminal ileum, right colon, and sigmoid colon biopsies from HIV-infected virally-suppressed individuals and found that gut glycomic patterns are associated with distinct microbial compositions and differential levels of chronic inflammation and HIV persistence.
32152415	4	57	theme	anti-inflammatory	881:897	arg1	glycans					911:917	the anti-inflammatory fucosylated glycans	877:917	the anti-inflammatory fucosylated glycans	877:917	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	2	58	theme	microbial	359:367	arg1	translocation					369:381	HIV-associated microbial translocation	344:381	HIV-associated microbial translocation	344:381	Nevertheless, it is unclear how gut glycosylation contributes to the HIV-associated microbial translocation and inflammation that persist despite viral suppression and contribute to the development of several comorbidities.
32152415	3	59	theme	virally-suppressed	585:602	arg1	individuals					604:614	HIV-infected virally-suppressed individuals	572:614	HIV-infected virally-suppressed individuals	572:614	We examined terminal ileum, right colon, and sigmoid colon biopsies from HIV-infected virally-suppressed individuals and found that gut glycomic patterns are associated with distinct microbial compositions and differential levels of chronic inflammation and HIV persistence.
32152415	3	60	theme	inflammation	740:751	arg1	compositions					692:703	distinct microbial compositions	673:703	distinct microbial compositions	673:703	We examined terminal ileum, right colon, and sigmoid colon biopsies from HIV-infected virally-suppressed individuals and found that gut glycomic patterns are associated with distinct microbial compositions and differential levels of chronic inflammation and HIV persistence.
32152415	3	60	theme	inflammation	740:751	arg1	levels					722:727	differential levels	709:727	differential levels of chronic inflammation and HIV persistence	709:771	We examined terminal ileum, right colon, and sigmoid colon biopsies from HIV-infected virally-suppressed individuals and found that gut glycomic patterns are associated with distinct microbial compositions and differential levels of chronic inflammation and HIV persistence.
32152415	4	61	theme	low	863:865	arg1	levels					867:872	low levels	863:872	low levels of the anti-inflammatory fucosylated glycans	863:917	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	4	62	theme	T-antigen	841:849	arg1	glycans					851:857	the pro-inflammatory hypo-sialylated T-antigen glycans	804:857	the pro-inflammatory hypo-sialylated T-antigen glycans	804:857	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	3	63	theme	colon	533:537	arg1	biopsies					558:565	terminal ileum, right colon, and sigmoid colon biopsies	511:565	terminal ileum, right colon, and sigmoid colon biopsies from HIV-infected virally-suppressed individuals	511:614	We examined terminal ileum, right colon, and sigmoid colon biopsies from HIV-infected virally-suppressed individuals and found that gut glycomic patterns are associated with distinct microbial compositions and differential levels of chronic inflammation and HIV persistence.
32152415	3	64	theme	right	527:531	arg1	colon					533:537	right colon	527:537	right colon	527:537	We examined terminal ileum, right colon, and sigmoid colon biopsies from HIV-infected virally-suppressed individuals and found that gut glycomic patterns are associated with distinct microbial compositions and differential levels of chronic inflammation and HIV persistence.
32152415	4	65	theme	inflammation	1078:1089	arg1	microbiome					1049:1058	a less diverse microbiome	1034:1058	a less diverse microbiome	1034:1058	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	4	65	theme	inflammation	1078:1089	arg1	levels					1068:1073	higher levels	1061:1073	higher levels of inflammation	1061:1089	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	4	65	theme	inflammation	1078:1089	arg1	levels					1103:1108	higher levels	1096:1108	higher levels of ileum-associated HIV DNA	1096:1136	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	4	65	theme	inflammation	1078:1089	arg1	species					987:993	glycan-degrading microbial species	960:993	glycan-degrading microbial species (in particular, Bacteroides vulgatus)	960:1031	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32152415	5	66	attach	linked	1158:1163	arg2	findings					1145:1152	These findings	1139:1152	These findings	1139:1152	These findings are linked to the activation of the inflammasome-mediating eIF2 signaling pathway.
32152415	5	66	attach	linked	1158:1163	arg1	activation					1172:1181	the activation	1168:1181	the activation of the inflammasome-mediating eIF2 signaling pathway	1168:1234	These findings are linked to the activation of the inflammasome-mediating eIF2 signaling pathway.
32152415	3	67	theme	distinct	673:680	arg1	compositions					692:703	distinct microbial compositions	673:703	distinct microbial compositions	673:703	We examined terminal ileum, right colon, and sigmoid colon biopsies from HIV-infected virally-suppressed individuals and found that gut glycomic patterns are associated with distinct microbial compositions and differential levels of chronic inflammation and HIV persistence.
32152415	1	68	theme	homeostatic	214:224	arg1	relationship					226:237	the homeostatic relationship	210:237	the homeostatic relationship between the gut and its microbiota	210:272	An emerging paradigm suggests that gut glycosylation is a key force in maintaining the homeostatic relationship between the gut and its microbiota.
32152415	0	69	theme	microbial	81:89	arg1	translocation					91:103	microbial translocation	81:103	microbial translocation	81:103	Sialylation and fucosylation modulate inflammasome-activating eIF2 Signaling and microbial translocation during HIV infection.
32152415	3	70	theme	microbial	682:690	arg1	compositions					692:703	distinct microbial compositions	673:703	distinct microbial compositions	673:703	We examined terminal ileum, right colon, and sigmoid colon biopsies from HIV-infected virally-suppressed individuals and found that gut glycomic patterns are associated with distinct microbial compositions and differential levels of chronic inflammation and HIV persistence.
32152415	6	71	theme	gut	1337:1339	arg1	factor					1329:1334	a previously unappreciated factor	1302:1334	a previously unappreciated factor	1302:1334	Our study thus provides the first proof-of-concept evidence that a previously unappreciated factor, gut glycosylation, is a force that may impact the vicious cycle between HIV infection, microbial translocation, and chronic inflammation.
32152415	6	71	theme	gut	1337:1339	arg1	glycosylation					1341:1353	gut glycosylation	1337:1353	gut glycosylation	1337:1353	Our study thus provides the first proof-of-concept evidence that a previously unappreciated factor, gut glycosylation, is a force that may impact the vicious cycle between HIV infection, microbial translocation, and chronic inflammation.
32152415	4	72	theme	microbiome	1049:1058	arg1	abundance					947:955	higher abundance	940:955	higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA	940:1136	In particular, high levels of the pro-inflammatory hypo-sialylated T-antigen glycans and low levels of the anti-inflammatory fucosylated glycans were associated with higher abundance of glycan-degrading microbial species (in particular, Bacteroides vulgatus), a less diverse microbiome, higher levels of inflammation, and higher levels of ileum-associated HIV DNA.
32915471	2	0	theme	matrix	382:387	arg1	composition					389:399	matrix composition	382:399	matrix composition	382:399	The goal of this study was to characterize the structure, matrix composition, and cell phenotype of the human CEP compared with adjacent tissues within the intervertebral joint: the nucleus pulposus (NP), annulus fibrosus (AF), and articular cartilage (AC).
32915471	7	1	from	cells	1176:1180	arg1	NP					1164:1165	NP	1164:1165	NP from CEP cells using collagen-10 (COLX)	1164:1205	We were able to distinguish NP from CEP cells using collagen-10 (COLX), highlighting COLX as a potential CEP marker.
32915471	3	2	theme	Isolated	582:589	arg1	tissues					611:617	Isolated CEP, NP, AF, and AC tissues	582:617	Isolated CEP, NP, AF, and AC tissues	582:617	Isolated CEP, NP, AF, and AC tissues and cells were evaluated for cell morphology, matrix composition, collagen structure, glycosaminoglycan content, and gene and protein expression.
32915471	0	3	theme	molecular	76:84	arg1	cell					87:90	the molecular, cell, and tissue levels	72:109	cell	87:90	Characterization of the human intervertebral disc cartilage endplate at the molecular, cell, and tissue levels.
32915471	2	4	dep	was	347:349	arg1	NP					524:525	NP	524:525	NP	524:525	The goal of this study was to characterize the structure, matrix composition, and cell phenotype of the human CEP compared with adjacent tissues within the intervertebral joint: the nucleus pulposus (NP), annulus fibrosus (AF), and articular cartilage (AC).
32915471	2	4	dep	was	347:349	arg1	annulus fibrosus					529:544	annulus fibrosus	529:544	annulus fibrosus (AF)	529:549	The goal of this study was to characterize the structure, matrix composition, and cell phenotype of the human CEP compared with adjacent tissues within the intervertebral joint: the nucleus pulposus (NP), annulus fibrosus (AF), and articular cartilage (AC).
32915471	2	4	dep	was	347:349	arg1	articular cartilage					556:574	articular cartilage	556:574	articular cartilage (AC)	556:579	The goal of this study was to characterize the structure, matrix composition, and cell phenotype of the human CEP compared with adjacent tissues within the intervertebral joint: the nucleus pulposus (NP), annulus fibrosus (AF), and articular cartilage (AC).
32915471	2	4	dep	was	347:349	arg1	AC					577:578	AC	577:578	AC	577:578	The goal of this study was to characterize the structure, matrix composition, and cell phenotype of the human CEP compared with adjacent tissues within the intervertebral joint: the nucleus pulposus (NP), annulus fibrosus (AF), and articular cartilage (AC).
32915471	2	4	dep	was	347:349	arg1	pulposus					514:521	the nucleus pulposus	502:521	the nucleus pulposus (NP)	502:526	The goal of this study was to characterize the structure, matrix composition, and cell phenotype of the human CEP compared with adjacent tissues within the intervertebral joint: the nucleus pulposus (NP), annulus fibrosus (AF), and articular cartilage (AC).
32915471	2	4	dep	was	347:349	arg1	AF					547:548	AF	547:548	AF	547:548	The goal of this study was to characterize the structure, matrix composition, and cell phenotype of the human CEP compared with adjacent tissues within the intervertebral joint: the nucleus pulposus (NP), annulus fibrosus (AF), and articular cartilage (AC).
32915471	1	5	theme	cartilage	140:148	arg1	CEP					160:162	CEP	160:162	CEP	160:162	Given the importance of the cartilage endplate (CEP) in low back pain (LBP), there is a need to characterize the human CEP at the molecular, cell, and tissue levels to inform treatment strategies that target it.
32915471	1	5	theme	cartilage	140:148	arg1	endplate					150:157	the cartilage endplate	136:157	the cartilage endplate (CEP)	136:163	Given the importance of the cartilage endplate (CEP) in low back pain (LBP), there is a need to characterize the human CEP at the molecular, cell, and tissue levels to inform treatment strategies that target it.
32915471	7	6	theme	CEP	1172:1174	arg1	cells					1176:1180	CEP cells	1172:1180	CEP cells using collagen-10 (COLX)	1172:1205	We were able to distinguish NP from CEP cells using collagen-10 (COLX), highlighting COLX as a potential CEP marker.
32915471	2	7	theme	intervertebral	480:493	arg1	joint					495:499	the intervertebral joint	476:499	the intervertebral joint	476:499	The goal of this study was to characterize the structure, matrix composition, and cell phenotype of the human CEP compared with adjacent tissues within the intervertebral joint: the nucleus pulposus (NP), annulus fibrosus (AF), and articular cartilage (AC).
32915471	6	8	theme	gene	1030:1033	arg1	expression					1035:1044	cell marker gene expression	1018:1044	cell marker gene expression	1018:1044	Significant differences in matrix and cell marker gene expression were observed between CEP and NP or AF, with the greatest differences between CEP and AC.
32915471	1	9	theme	tissue	263:268	arg1	levels					270:275	the molecular, cell, and tissue levels	238:275	levels	270:275	Given the importance of the cartilage endplate (CEP) in low back pain (LBP), there is a need to characterize the human CEP at the molecular, cell, and tissue levels to inform treatment strategies that target it.
32915471	1	10	from	importance	122:131	arg1	LBP					183:185	LBP	183:185	LBP	183:185	Given the importance of the cartilage endplate (CEP) in low back pain (LBP), there is a need to characterize the human CEP at the molecular, cell, and tissue levels to inform treatment strategies that target it.
32915471	1	10	from	importance	122:131	arg1	pain					177:180	low back pain	168:180	low back pain (LBP)	168:186	Given the importance of the cartilage endplate (CEP) in low back pain (LBP), there is a need to characterize the human CEP at the molecular, cell, and tissue levels to inform treatment strategies that target it.
32915471	3	11	theme	gene	736:739	arg1	expression					753:762	gene and protein expression	736:762	expression	753:762	Isolated CEP, NP, AF, and AC tissues and cells were evaluated for cell morphology, matrix composition, collagen structure, glycosaminoglycan content, and gene and protein expression.
32915471	3	12	theme	cell	648:651	arg1	morphology					653:662	cell morphology	648:662	cell morphology	648:662	Isolated CEP, NP, AF, and AC tissues and cells were evaluated for cell morphology, matrix composition, collagen structure, glycosaminoglycan content, and gene and protein expression.
32915471	2	13	theme	cell	406:409	arg1	phenotype					411:419	cell phenotype	406:419	cell phenotype	406:419	The goal of this study was to characterize the structure, matrix composition, and cell phenotype of the human CEP compared with adjacent tissues within the intervertebral joint: the nucleus pulposus (NP), annulus fibrosus (AF), and articular cartilage (AC).
32915471	3	14	theme	CEP	591:593	arg1	tissues					611:617	Isolated CEP, NP, AF, and AC tissues	582:617	Isolated CEP, NP, AF, and AC tissues	582:617	Isolated CEP, NP, AF, and AC tissues and cells were evaluated for cell morphology, matrix composition, collagen structure, glycosaminoglycan content, and gene and protein expression.
32915471	7	15	theme	CEP	1241:1243	arg1	marker					1245:1250	a potential CEP marker	1229:1250	a potential CEP marker	1229:1250	We were able to distinguish NP from CEP cells using collagen-10 (COLX), highlighting COLX as a potential CEP marker.
32915471	7	15	theme	CEP	1241:1243	arg1	COLX					1221:1224	COLX	1221:1224	COLX	1221:1224	We were able to distinguish NP from CEP cells using collagen-10 (COLX), highlighting COLX as a potential CEP marker.
32915471	1	16	theme	endplate	150:157	arg1	importance					122:131	the importance	118:131	the importance of the cartilage endplate (CEP) in low back pain (LBP)	118:186	Given the importance of the cartilage endplate (CEP) in low back pain (LBP), there is a need to characterize the human CEP at the molecular, cell, and tissue levels to inform treatment strategies that target it.
32915471	1	17	from	cell	253:256	arg1	CEP					231:233	the human CEP	221:233	the human CEP at the molecular, cell, and tissue levels to inform treatment strategies that target it	221:321	Given the importance of the cartilage endplate (CEP) in low back pain (LBP), there is a need to characterize the human CEP at the molecular, cell, and tissue levels to inform treatment strategies that target it.
32915471	9	18	from	joint	1590:1594	arg1	LBP					1607:1609	chronic LBP	1599:1609	chronic LBP	1599:1609	This study highlights a unique structure, matrix composition, and cell phenotype for the human CEP and can help to inform regenerative strategies that target the intervertebral disc joint in chronic LBP.
32915471	4	19	theme	interterritorial	835:850	arg1	matrix					852:857	a collagen-rich interterritorial matrix	819:857	a collagen-rich interterritorial matrix	819:857	The CEP contained elongated cells that mainly produce a collagen-rich interterritorial matrix and a proteoglycan-rich territorial matrix.
32915471	0	20	theme	tissue	97:102	arg1	levels					104:109	the molecular, cell, and tissue levels	72:109	levels	104:109	Characterization of the human intervertebral disc cartilage endplate at the molecular, cell, and tissue levels.
32915471	4	21	theme	proteoglycan-rich	865:881	arg1	matrix					895:900	a proteoglycan-rich territorial matrix	863:900	a proteoglycan-rich territorial matrix	863:900	The CEP contained elongated cells that mainly produce a collagen-rich interterritorial matrix and a proteoglycan-rich territorial matrix.
32915471	3	22	theme	NP	596:597	arg1	tissues					611:617	Isolated CEP, NP, AF, and AC tissues	582:617	Isolated CEP, NP, AF, and AC tissues	582:617	Isolated CEP, NP, AF, and AC tissues and cells were evaluated for cell morphology, matrix composition, collagen structure, glycosaminoglycan content, and gene and protein expression.
32915471	2	23	theme	nucleus	506:512	arg1	NP					524:525	NP	524:525	NP	524:525	The goal of this study was to characterize the structure, matrix composition, and cell phenotype of the human CEP compared with adjacent tissues within the intervertebral joint: the nucleus pulposus (NP), annulus fibrosus (AF), and articular cartilage (AC).
32915471	2	23	theme	nucleus	506:512	arg1	pulposus					514:521	the nucleus pulposus	502:521	the nucleus pulposus (NP)	502:526	The goal of this study was to characterize the structure, matrix composition, and cell phenotype of the human CEP compared with adjacent tissues within the intervertebral joint: the nucleus pulposus (NP), annulus fibrosus (AF), and articular cartilage (AC).
32915471	5	24	contain	contained	911:919	arg1	CEP					907:909	The CEP	903:909	The CEP	903:909	The CEP contained significantly fewer glycosaminoglycans than the NP tissue.
32915471	5	24	contain	contained	911:919	arg2	glycosaminoglycans					941:958	significantly fewer glycosaminoglycans	921:958	significantly fewer glycosaminoglycans	921:958	The CEP contained significantly fewer glycosaminoglycans than the NP tissue.
32915471	5	25	theme	NP	969:970	arg1	tissue					972:977	the NP tissue	965:977	the NP tissue	965:977	The CEP contained significantly fewer glycosaminoglycans than the NP tissue.
32915471	2	26	theme	study	341:345	arg1	goal					328:331	The goal	324:331	The goal of this study	324:345	The goal of this study was to characterize the structure, matrix composition, and cell phenotype of the human CEP compared with adjacent tissues within the intervertebral joint: the nucleus pulposus (NP), annulus fibrosus (AF), and articular cartilage (AC).
32915471	6	27	theme	greatest	1095:1102	arg1	differences					1104:1114	the greatest differences	1091:1114	the greatest differences between CEP and AC	1091:1133	Significant differences in matrix and cell marker gene expression were observed between CEP and NP or AF, with the greatest differences between CEP and AC.
32915471	6	28	theme	marker	1023:1028	arg1	expression					1035:1044	cell marker gene expression	1018:1044	cell marker gene expression	1018:1044	Significant differences in matrix and cell marker gene expression were observed between CEP and NP or AF, with the greatest differences between CEP and AC.
32915471	0	29	theme	human	24:28	arg1	cartilage					50:58	the human intervertebral disc cartilage	20:58	the human intervertebral disc cartilage	20:58	Characterization of the human intervertebral disc cartilage endplate at the molecular, cell, and tissue levels.
32915471	3	30	theme	AF	600:601	arg1	tissues					611:617	Isolated CEP, NP, AF, and AC tissues	582:617	Isolated CEP, NP, AF, and AC tissues	582:617	Isolated CEP, NP, AF, and AC tissues and cells were evaluated for cell morphology, matrix composition, collagen structure, glycosaminoglycan content, and gene and protein expression.
32915471	9	31	theme	cell	1474:1477	arg1	phenotype					1479:1487	cell phenotype	1474:1487	cell phenotype	1474:1487	This study highlights a unique structure, matrix composition, and cell phenotype for the human CEP and can help to inform regenerative strategies that target the intervertebral disc joint in chronic LBP.
32915471	6	32	theme	cell	1018:1021	arg1	expression					1035:1044	cell marker gene expression	1018:1044	cell marker gene expression	1018:1044	Significant differences in matrix and cell marker gene expression were observed between CEP and NP or AF, with the greatest differences between CEP and AC.
32915471	1	33	from	levels	270:275	arg1	CEP					231:233	the human CEP	221:233	the human CEP at the molecular, cell, and tissue levels to inform treatment strategies that target it	221:321	Given the importance of the cartilage endplate (CEP) in low back pain (LBP), there is a need to characterize the human CEP at the molecular, cell, and tissue levels to inform treatment strategies that target it.
32915471	7	34	theme	potential	1231:1239	arg1	marker					1245:1250	a potential CEP marker	1229:1250	a potential CEP marker	1229:1250	We were able to distinguish NP from CEP cells using collagen-10 (COLX), highlighting COLX as a potential CEP marker.
32915471	7	34	theme	potential	1231:1239	arg1	COLX					1221:1224	COLX	1221:1224	COLX	1221:1224	We were able to distinguish NP from CEP cells using collagen-10 (COLX), highlighting COLX as a potential CEP marker.
32915471	2	35	theme	CEP	434:436	arg1	composition					389:399	matrix composition	382:399	matrix composition	382:399	The goal of this study was to characterize the structure, matrix composition, and cell phenotype of the human CEP compared with adjacent tissues within the intervertebral joint: the nucleus pulposus (NP), annulus fibrosus (AF), and articular cartilage (AC).
32915471	2	35	theme	CEP	434:436	arg1	phenotype					411:419	cell phenotype	406:419	cell phenotype	406:419	The goal of this study was to characterize the structure, matrix composition, and cell phenotype of the human CEP compared with adjacent tissues within the intervertebral joint: the nucleus pulposus (NP), annulus fibrosus (AF), and articular cartilage (AC).
32915471	2	35	theme	CEP	434:436	arg1	structure					371:379	the structure	367:379	the structure	367:379	The goal of this study was to characterize the structure, matrix composition, and cell phenotype of the human CEP compared with adjacent tissues within the intervertebral joint: the nucleus pulposus (NP), annulus fibrosus (AF), and articular cartilage (AC).
32915471	1	36	theme	treatment	287:295	arg1	strategies					297:306	treatment strategies	287:306	treatment strategies that target it	287:321	Given the importance of the cartilage endplate (CEP) in low back pain (LBP), there is a need to characterize the human CEP at the molecular, cell, and tissue levels to inform treatment strategies that target it.
32915471	3	37	theme	protein	745:751	arg1	expression					753:762	gene and protein expression	736:762	expression	753:762	Isolated CEP, NP, AF, and AC tissues and cells were evaluated for cell morphology, matrix composition, collagen structure, glycosaminoglycan content, and gene and protein expression.
32915471	1	38	theme	low	168:170	arg1	LBP					183:185	LBP	183:185	LBP	183:185	Given the importance of the cartilage endplate (CEP) in low back pain (LBP), there is a need to characterize the human CEP at the molecular, cell, and tissue levels to inform treatment strategies that target it.
32915471	1	38	theme	low	168:170	arg1	pain					177:180	low back pain	168:180	low back pain (LBP)	168:186	Given the importance of the cartilage endplate (CEP) in low back pain (LBP), there is a need to characterize the human CEP at the molecular, cell, and tissue levels to inform treatment strategies that target it.
32915471	0	39	theme	disc	45:48	arg1	cartilage					50:58	the human intervertebral disc cartilage	20:58	the human intervertebral disc cartilage	20:58	Characterization of the human intervertebral disc cartilage endplate at the molecular, cell, and tissue levels.
32915471	1	40	theme	human	225:229	arg1	CEP					231:233	the human CEP	221:233	the human CEP at the molecular, cell, and tissue levels to inform treatment strategies that target it	221:321	Given the importance of the cartilage endplate (CEP) in low back pain (LBP), there is a need to characterize the human CEP at the molecular, cell, and tissue levels to inform treatment strategies that target it.
32915471	2	41	theme	human	428:432	arg1	CEP					434:436	the human CEP	424:436	the human CEP	424:436	The goal of this study was to characterize the structure, matrix composition, and cell phenotype of the human CEP compared with adjacent tissues within the intervertebral joint: the nucleus pulposus (NP), annulus fibrosus (AF), and articular cartilage (AC).
32915471	5	42	theme	fewer	935:939	arg1	glycosaminoglycans					941:958	significantly fewer glycosaminoglycans	921:958	significantly fewer glycosaminoglycans	921:958	The CEP contained significantly fewer glycosaminoglycans than the NP tissue.
32915471	9	43	theme	intervertebral	1570:1583	arg1	joint					1590:1594	the intervertebral disc joint	1566:1594	the intervertebral disc joint in chronic LBP	1566:1609	This study highlights a unique structure, matrix composition, and cell phenotype for the human CEP and can help to inform regenerative strategies that target the intervertebral disc joint in chronic LBP.
32915471	0	44	theme	intervertebral	30:43	arg1	cartilage					50:58	the human intervertebral disc cartilage	20:58	the human intervertebral disc cartilage	20:58	Characterization of the human intervertebral disc cartilage endplate at the molecular, cell, and tissue levels.
32915471	3	45	theme	AC	608:609	arg1	tissues					611:617	Isolated CEP, NP, AF, and AC tissues	582:617	Isolated CEP, NP, AF, and AC tissues	582:617	Isolated CEP, NP, AF, and AC tissues and cells were evaluated for cell morphology, matrix composition, collagen structure, glycosaminoglycan content, and gene and protein expression.
32915471	4	46	theme	collagen-rich	821:833	arg1	matrix					852:857	a collagen-rich interterritorial matrix	819:857	a collagen-rich interterritorial matrix	819:857	The CEP contained elongated cells that mainly produce a collagen-rich interterritorial matrix and a proteoglycan-rich territorial matrix.
32915471	9	47	theme	chronic	1599:1605	arg1	LBP					1607:1609	chronic LBP	1599:1609	chronic LBP	1599:1609	This study highlights a unique structure, matrix composition, and cell phenotype for the human CEP and can help to inform regenerative strategies that target the intervertebral disc joint in chronic LBP.
32915471	6	48	from	differences	992:1002	arg1	expression					1035:1044	cell marker gene expression	1018:1044	cell marker gene expression	1018:1044	Significant differences in matrix and cell marker gene expression were observed between CEP and NP or AF, with the greatest differences between CEP and AC.
32915471	6	48	from	differences	992:1002	arg1	matrix					1007:1012	matrix	1007:1012	matrix	1007:1012	Significant differences in matrix and cell marker gene expression were observed between CEP and NP or AF, with the greatest differences between CEP and AC.
32915471	4	49	theme	elongated	783:791	arg1	cells					793:797	elongated cells	783:797	elongated cells that mainly produce a collagen-rich interterritorial matrix and a proteoglycan-rich territorial matrix	783:900	The CEP contained elongated cells that mainly produce a collagen-rich interterritorial matrix and a proteoglycan-rich territorial matrix.
32915471	0	50	theme	cartilage	50:58	arg1	Characterization					0:15	Characterization	0:15	Characterization of the human intervertebral disc cartilage	0:58	Characterization of the human intervertebral disc cartilage endplate at the molecular, cell, and tissue levels.
32915471	9	51	theme	human	1497:1501	arg1	CEP					1503:1505	the human CEP	1493:1505	the human CEP	1493:1505	This study highlights a unique structure, matrix composition, and cell phenotype for the human CEP and can help to inform regenerative strategies that target the intervertebral disc joint in chronic LBP.
32915471	9	52	theme	matrix	1450:1455	arg1	composition					1457:1467	matrix composition	1450:1467	matrix composition	1450:1467	This study highlights a unique structure, matrix composition, and cell phenotype for the human CEP and can help to inform regenerative strategies that target the intervertebral disc joint in chronic LBP.
32915471	1	53	theme	molecular	242:250	arg1	cell					253:256	the molecular, cell, and tissue levels	238:275	cell	253:256	Given the importance of the cartilage endplate (CEP) in low back pain (LBP), there is a need to characterize the human CEP at the molecular, cell, and tissue levels to inform treatment strategies that target it.
32915471	2	54	theme	adjacent	452:459	arg1	tissues					461:467	adjacent tissues	452:467	adjacent tissues within the intervertebral joint	452:499	The goal of this study was to characterize the structure, matrix composition, and cell phenotype of the human CEP compared with adjacent tissues within the intervertebral joint: the nucleus pulposus (NP), annulus fibrosus (AF), and articular cartilage (AC).
32915471	8	55	theme	cell	1286:1289	arg1	levels					1302:1307	the cell and tissue levels	1282:1307	levels	1302:1307	Our findings suggest that at the cell and tissue levels, the CEP demonstrates both similarities and differences when compared with NP, AF, and hyaline AC.
32915471	4	56	contain	contained	773:781	arg1	CEP					769:771	The CEP	765:771	The CEP	765:771	The CEP contained elongated cells that mainly produce a collagen-rich interterritorial matrix and a proteoglycan-rich territorial matrix.
32915471	4	56	contain	contained	773:781	arg2	cells					793:797	elongated cells	783:797	elongated cells that mainly produce a collagen-rich interterritorial matrix and a proteoglycan-rich territorial matrix	783:900	The CEP contained elongated cells that mainly produce a collagen-rich interterritorial matrix and a proteoglycan-rich territorial matrix.
32915471	3	57	theme	collagen	685:692	arg1	structure					694:702	collagen structure	685:702	collagen structure	685:702	Isolated CEP, NP, AF, and AC tissues and cells were evaluated for cell morphology, matrix composition, collagen structure, glycosaminoglycan content, and gene and protein expression.
32915471	9	58	theme	disc	1585:1588	arg1	joint					1590:1594	the intervertebral disc joint	1566:1594	the intervertebral disc joint in chronic LBP	1566:1609	This study highlights a unique structure, matrix composition, and cell phenotype for the human CEP and can help to inform regenerative strategies that target the intervertebral disc joint in chronic LBP.
32915471	6	59	theme	Significant	980:990	arg1	differences					992:1002	Significant differences	980:1002	Significant differences in matrix and cell marker gene expression	980:1044	Significant differences in matrix and cell marker gene expression were observed between CEP and NP or AF, with the greatest differences between CEP and AC.
32915471	3	60	theme	matrix	665:670	arg1	composition					672:682	matrix composition	665:682	matrix composition	665:682	Isolated CEP, NP, AF, and AC tissues and cells were evaluated for cell morphology, matrix composition, collagen structure, glycosaminoglycan content, and gene and protein expression.
32915471	8	61	theme	hyaline	1396:1402	arg1	AC					1404:1405	hyaline AC	1396:1405	hyaline AC	1396:1405	Our findings suggest that at the cell and tissue levels, the CEP demonstrates both similarities and differences when compared with NP, AF, and hyaline AC.
32915471	8	62	theme	tissue	1295:1300	arg1	levels					1302:1307	the cell and tissue levels	1282:1307	levels	1302:1307	Our findings suggest that at the cell and tissue levels, the CEP demonstrates both similarities and differences when compared with NP, AF, and hyaline AC.
32915471	9	63	theme	regenerative	1530:1541	arg1	strategies					1543:1552	regenerative strategies	1530:1552	regenerative strategies that target the intervertebral disc joint in chronic LBP	1530:1609	This study highlights a unique structure, matrix composition, and cell phenotype for the human CEP and can help to inform regenerative strategies that target the intervertebral disc joint in chronic LBP.
32915471	4	64	theme	territorial	883:893	arg1	matrix					895:900	a proteoglycan-rich territorial matrix	863:900	a proteoglycan-rich territorial matrix	863:900	The CEP contained elongated cells that mainly produce a collagen-rich interterritorial matrix and a proteoglycan-rich territorial matrix.
32915471	3	65	theme	glycosaminoglycan	705:721	arg1	content					723:729	glycosaminoglycan content	705:729	glycosaminoglycan content	705:729	Isolated CEP, NP, AF, and AC tissues and cells were evaluated for cell morphology, matrix composition, collagen structure, glycosaminoglycan content, and gene and protein expression.
32915471	9	66	theme	unique	1432:1437	arg1	structure					1439:1447	a unique structure	1430:1447	a unique structure	1430:1447	This study highlights a unique structure, matrix composition, and cell phenotype for the human CEP and can help to inform regenerative strategies that target the intervertebral disc joint in chronic LBP.
32084468	0	0	theme	Congo	86:90	arg1	dye					96:98	Congo red dye	86:98	Congo red dye	86:98	Surface modification of nanocellulose using polypyrrole for the adsorptive removal of Congo red dye and chromium in binary mixture.
32084468	7	1	theme	Cr	1059:1060	arg1	biosorption					1044:1054	the biosorption	1040:1054	the biosorption of Cr(VI) and CR on NCPPY	1040:1080	Thermodynamic data showed that the biosorption of Cr(VI) and CR on NCPPY is an endothermic, spontaneous, and entropy-driven process.
32084468	7	1	theme	Cr	1059:1060	arg1	process					1133:1139	an endothermic, spontaneous, and entropy-driven process	1085:1139	an endothermic, spontaneous, and entropy-driven process	1085:1139	Thermodynamic data showed that the biosorption of Cr(VI) and CR on NCPPY is an endothermic, spontaneous, and entropy-driven process.
32084468	10	2	contain	has	1355:1357	arg2	potential					1365:1373	great potential	1359:1373	great potential	1359:1373	Results indicated that NCPPY has great potential to remove Cr(VI) and CR binary mixture under simulated conditions.
32084468	10	2	contain	has	1355:1357	arg1	NCPPY					1349:1353	NCPPY	1349:1353	NCPPY	1349:1353	Results indicated that NCPPY has great potential to remove Cr(VI) and CR binary mixture under simulated conditions.
32084468	3	3	theme	excellent	473:481	arg1	stability					491:499	excellent thermal stability	473:499	excellent thermal stability	473:499	These analysis showed the conversion of cellulose to nano sized crystalline structure with excellent thermal stability and higher surface area.
32084468	1	4	theme	polypyrrole	230:240	arg1	matrix					253:258	polypyrrole supporting matrix	230:258	polypyrrole supporting matrix	230:258	In this study, nanocellulose was synthesised by acid hydrolysis of cellulose and was coupled with polypyrrole supporting matrix.
32084468	6	5	theme	Langmuir	911:918	arg1	isotherm					935:942	The Langmuir and Freundlich isotherm	907:942	isotherm	935:942	The Langmuir and Freundlich isotherm well fitted the equilibrium data for CR and Cr(VI) respectively.
32084468	6	6	theme	equilibrium	960:970	arg1	data					972:975	the equilibrium data	956:975	the equilibrium data for CR and Cr(VI)	956:993	The Langmuir and Freundlich isotherm well fitted the equilibrium data for CR and Cr(VI) respectively.
32084468	3	7	theme	thermal	483:489	arg1	stability					491:499	excellent thermal stability	473:499	excellent thermal stability	473:499	These analysis showed the conversion of cellulose to nano sized crystalline structure with excellent thermal stability and higher surface area.
32084468	1	8	theme	supporting	242:251	arg1	matrix					253:258	polypyrrole supporting matrix	230:258	polypyrrole supporting matrix	230:258	In this study, nanocellulose was synthesised by acid hydrolysis of cellulose and was coupled with polypyrrole supporting matrix.
32084468	0	9	theme	dye	96:98	arg1	removal					75:81	the adsorptive removal	60:81	the adsorptive removal of Congo red dye	60:98	Surface modification of nanocellulose using polypyrrole for the adsorptive removal of Congo red dye and chromium in binary mixture.
32084468	2	10	theme	polypyrrole	291:301	arg1	NCPPY					314:318	The synthesised nanocellulose polypyrrole composite (NCPPY)	261:319	The synthesised nanocellulose polypyrrole composite (NCPPY)	261:319	The synthesised nanocellulose polypyrrole composite (NCPPY) was characterised by FESEM, XRD, FTIR, BET, TGA/DSC and NMR.
32084468	0	11	theme	red	92:94	arg1	dye					96:98	Congo red dye	86:98	Congo red dye	86:98	Surface modification of nanocellulose using polypyrrole for the adsorptive removal of Congo red dye and chromium in binary mixture.
32084468	5	12	theme	removal	846:852	arg1	efficiency					854:863	the maximum removal efficiency	834:863	the maximum removal efficiency	834:863	Response Surface Methodology (RSM) has been employed as an optimization tool for the efficient removal of Cr(VI) and CR and the maximum removal efficiency was found to be 80% and 85% respectively.
32084468	5	12	theme	removal	846:852	arg1	%					883:883	80%	881:883	80%	881:883	Response Surface Methodology (RSM) has been employed as an optimization tool for the efficient removal of Cr(VI) and CR and the maximum removal efficiency was found to be 80% and 85% respectively.
32084468	7	13	theme	CR	1070:1071	arg1	biosorption					1044:1054	the biosorption	1040:1054	the biosorption of Cr(VI) and CR on NCPPY	1040:1080	Thermodynamic data showed that the biosorption of Cr(VI) and CR on NCPPY is an endothermic, spontaneous, and entropy-driven process.
32084468	7	13	theme	CR	1070:1071	arg1	process					1133:1139	an endothermic, spontaneous, and entropy-driven process	1085:1139	an endothermic, spontaneous, and entropy-driven process	1085:1139	Thermodynamic data showed that the biosorption of Cr(VI) and CR on NCPPY is an endothermic, spontaneous, and entropy-driven process.
32084468	2	14	theme	nanocellulose	277:289	arg1	NCPPY					314:318	The synthesised nanocellulose polypyrrole composite (NCPPY)	261:319	The synthesised nanocellulose polypyrrole composite (NCPPY)	261:319	The synthesised nanocellulose polypyrrole composite (NCPPY) was characterised by FESEM, XRD, FTIR, BET, TGA/DSC and NMR.
32084468	7	15	theme	endothermic	1088:1098	arg1	biosorption					1044:1054	the biosorption	1040:1054	the biosorption of Cr(VI) and CR on NCPPY	1040:1080	Thermodynamic data showed that the biosorption of Cr(VI) and CR on NCPPY is an endothermic, spontaneous, and entropy-driven process.
32084468	7	15	theme	endothermic	1088:1098	arg1	process					1133:1139	an endothermic, spontaneous, and entropy-driven process	1085:1139	an endothermic, spontaneous, and entropy-driven process	1085:1139	Thermodynamic data showed that the biosorption of Cr(VI) and CR on NCPPY is an endothermic, spontaneous, and entropy-driven process.
32084468	7	16	theme	entropy-driven	1118:1131	arg1	biosorption					1044:1054	the biosorption	1040:1054	the biosorption of Cr(VI) and CR on NCPPY	1040:1080	Thermodynamic data showed that the biosorption of Cr(VI) and CR on NCPPY is an endothermic, spontaneous, and entropy-driven process.
32084468	7	16	theme	entropy-driven	1118:1131	arg1	process					1133:1139	an endothermic, spontaneous, and entropy-driven process	1085:1139	an endothermic, spontaneous, and entropy-driven process	1085:1139	Thermodynamic data showed that the biosorption of Cr(VI) and CR on NCPPY is an endothermic, spontaneous, and entropy-driven process.
32084468	2	17	theme	synthesised	265:275	arg1	NCPPY					314:318	The synthesised nanocellulose polypyrrole composite (NCPPY)	261:319	The synthesised nanocellulose polypyrrole composite (NCPPY)	261:319	The synthesised nanocellulose polypyrrole composite (NCPPY) was characterised by FESEM, XRD, FTIR, BET, TGA/DSC and NMR.
32084468	6	18	theme	Freundlich	924:933	arg1	isotherm					935:942	The Langmuir and Freundlich isotherm	907:942	isotherm	935:942	The Langmuir and Freundlich isotherm well fitted the equilibrium data for CR and Cr(VI) respectively.
32084468	9	19	theme	co-existing	1251:1261	arg1	ions					1263:1266	co-existing ions	1251:1266	co-existing ions	1251:1266	Effect of co-existing ions were checked using several common salts and heavy metals.
32084468	0	20	from	polypyrrole	44:54	arg1	mixture					123:129	binary mixture	116:129	binary mixture	116:129	Surface modification of nanocellulose using polypyrrole for the adsorptive removal of Congo red dye and chromium in binary mixture.
32084468	3	21	theme	surface	512:518	arg1	area					520:523	higher surface area	505:523	higher surface area	505:523	These analysis showed the conversion of cellulose to nano sized crystalline structure with excellent thermal stability and higher surface area.
32084468	4	22	theme	initial	618:624	arg1	concentration					626:638	initial concentration	618:638	initial concentration of Chromium (Cr(VI)) and Congo Red (CR)	618:678	The effect of different parameters like pH, temperature, contact time, adsorbent dosage and initial concentration of Chromium (Cr(VI)) and Congo Red (CR) were optimised in batch mode.
32084468	10	23	theme	binary	1399:1404	arg1	mixture					1406:1412	CR binary mixture	1396:1412	CR binary mixture	1396:1412	Results indicated that NCPPY has great potential to remove Cr(VI) and CR binary mixture under simulated conditions.
32084468	10	24	theme	CR	1396:1397	arg1	mixture					1406:1412	CR binary mixture	1396:1412	CR binary mixture	1396:1412	Results indicated that NCPPY has great potential to remove Cr(VI) and CR binary mixture under simulated conditions.
32084468	4	25	theme	Chromium	643:650	arg1	pH					566:567	pH	566:567	pH	566:567	The effect of different parameters like pH, temperature, contact time, adsorbent dosage and initial concentration of Chromium (Cr(VI)) and Congo Red (CR) were optimised in batch mode.
32084468	4	25	theme	Chromium	643:650	arg1	dosage					607:612	adsorbent dosage	597:612	adsorbent dosage	597:612	The effect of different parameters like pH, temperature, contact time, adsorbent dosage and initial concentration of Chromium (Cr(VI)) and Congo Red (CR) were optimised in batch mode.
32084468	4	25	theme	Chromium	643:650	arg1	concentration					626:638	initial concentration	618:638	initial concentration of Chromium (Cr(VI)) and Congo Red (CR)	618:678	The effect of different parameters like pH, temperature, contact time, adsorbent dosage and initial concentration of Chromium (Cr(VI)) and Congo Red (CR) were optimised in batch mode.
32084468	4	25	theme	Chromium	643:650	arg1	temperature					570:580	temperature	570:580	temperature	570:580	The effect of different parameters like pH, temperature, contact time, adsorbent dosage and initial concentration of Chromium (Cr(VI)) and Congo Red (CR) were optimised in batch mode.
32084468	4	25	theme	Chromium	643:650	arg1	time					591:594	contact time	583:594	contact time	583:594	The effect of different parameters like pH, temperature, contact time, adsorbent dosage and initial concentration of Chromium (Cr(VI)) and Congo Red (CR) were optimised in batch mode.
32084468	0	26	theme	Surface	0:6	arg1	modification					8:19	Surface modification	0:19	Surface modification of nanocellulose	0:36	Surface modification of nanocellulose using polypyrrole for the adsorptive removal of Congo red dye and chromium in binary mixture.
32084468	4	27	dep	Cr	653:654	arg1	VI					656:657	VI	656:657	VI	656:657	The effect of different parameters like pH, temperature, contact time, adsorbent dosage and initial concentration of Chromium (Cr(VI)) and Congo Red (CR) were optimised in batch mode.
32084468	10	28	dep	Cr	1385:1386	arg1	VI					1388:1389	VI	1388:1389	VI	1388:1389	Results indicated that NCPPY has great potential to remove Cr(VI) and CR binary mixture under simulated conditions.
32084468	4	29	theme	Red	671:673	arg1	pH					566:567	pH	566:567	pH	566:567	The effect of different parameters like pH, temperature, contact time, adsorbent dosage and initial concentration of Chromium (Cr(VI)) and Congo Red (CR) were optimised in batch mode.
32084468	4	29	theme	Red	671:673	arg1	dosage					607:612	adsorbent dosage	597:612	adsorbent dosage	597:612	The effect of different parameters like pH, temperature, contact time, adsorbent dosage and initial concentration of Chromium (Cr(VI)) and Congo Red (CR) were optimised in batch mode.
32084468	4	29	theme	Red	671:673	arg1	concentration					626:638	initial concentration	618:638	initial concentration of Chromium (Cr(VI)) and Congo Red (CR)	618:678	The effect of different parameters like pH, temperature, contact time, adsorbent dosage and initial concentration of Chromium (Cr(VI)) and Congo Red (CR) were optimised in batch mode.
32084468	4	29	theme	Red	671:673	arg1	temperature					570:580	temperature	570:580	temperature	570:580	The effect of different parameters like pH, temperature, contact time, adsorbent dosage and initial concentration of Chromium (Cr(VI)) and Congo Red (CR) were optimised in batch mode.
32084468	4	29	theme	Red	671:673	arg1	time					591:594	contact time	583:594	contact time	583:594	The effect of different parameters like pH, temperature, contact time, adsorbent dosage and initial concentration of Chromium (Cr(VI)) and Congo Red (CR) were optimised in batch mode.
32084468	8	30	dep	Cr	1198:1199	arg1	VI					1201:1202	VI	1201:1202	VI	1201:1202	The adsorption kinetic followed pseudo-second-order for Cr(VI) and intraparticle diffusion for CR.
32084468	0	31	theme	nanocellulose	24:36	arg1	modification					8:19	Surface modification	0:19	Surface modification of nanocellulose	0:36	Surface modification of nanocellulose using polypyrrole for the adsorptive removal of Congo red dye and chromium in binary mixture.
32084468	0	32	theme	binary	116:121	arg1	mixture					123:129	binary mixture	116:129	binary mixture	116:129	Surface modification of nanocellulose using polypyrrole for the adsorptive removal of Congo red dye and chromium in binary mixture.
32084468	1	33	theme	acid	180:183	arg1	hydrolysis					185:194	acid hydrolysis	180:194	acid hydrolysis of cellulose	180:207	In this study, nanocellulose was synthesised by acid hydrolysis of cellulose and was coupled with polypyrrole supporting matrix.
32084468	5	34	theme	CR	827:828	arg1	removal					805:811	the efficient removal	791:811	the efficient removal of Cr(VI) and CR	791:828	Response Surface Methodology (RSM) has been employed as an optimization tool for the efficient removal of Cr(VI) and CR and the maximum removal efficiency was found to be 80% and 85% respectively.
32084468	4	35	theme	different	540:548	arg1	parameters					550:559	different parameters	540:559	different parameters like pH, temperature, contact time, adsorbent dosage and initial concentration of Chromium (Cr(VI)) and Congo Red (CR)	540:678	The effect of different parameters like pH, temperature, contact time, adsorbent dosage and initial concentration of Chromium (Cr(VI)) and Congo Red (CR) were optimised in batch mode.
32084468	3	36	theme	higher	505:510	arg1	area					520:523	higher surface area	505:523	higher surface area	505:523	These analysis showed the conversion of cellulose to nano sized crystalline structure with excellent thermal stability and higher surface area.
32084468	2	37	theme	composite	303:311	arg1	NCPPY					314:318	The synthesised nanocellulose polypyrrole composite (NCPPY)	261:319	The synthesised nanocellulose polypyrrole composite (NCPPY)	261:319	The synthesised nanocellulose polypyrrole composite (NCPPY) was characterised by FESEM, XRD, FTIR, BET, TGA/DSC and NMR.
32084468	10	38	theme	great	1359:1363	arg1	potential					1365:1373	great potential	1359:1373	great potential	1359:1373	Results indicated that NCPPY has great potential to remove Cr(VI) and CR binary mixture under simulated conditions.
32084468	3	39	theme	nano	435:438	arg1	structure					458:466	nano sized crystalline structure	435:466	nano sized crystalline structure	435:466	These analysis showed the conversion of cellulose to nano sized crystalline structure with excellent thermal stability and higher surface area.
32084468	9	40	theme	common	1295:1300	arg1	salts					1302:1306	several common salts	1287:1306	several common salts	1287:1306	Effect of co-existing ions were checked using several common salts and heavy metals.
32084468	8	41	theme	intraparticle	1209:1221	arg1	diffusion					1223:1231	intraparticle diffusion	1209:1231	intraparticle diffusion	1209:1231	The adsorption kinetic followed pseudo-second-order for Cr(VI) and intraparticle diffusion for CR.
32084468	7	42	theme	spontaneous	1101:1111	arg1	biosorption					1044:1054	the biosorption	1040:1054	the biosorption of Cr(VI) and CR on NCPPY	1040:1080	Thermodynamic data showed that the biosorption of Cr(VI) and CR on NCPPY is an endothermic, spontaneous, and entropy-driven process.
32084468	7	42	theme	spontaneous	1101:1111	arg1	process					1133:1139	an endothermic, spontaneous, and entropy-driven process	1085:1139	an endothermic, spontaneous, and entropy-driven process	1085:1139	Thermodynamic data showed that the biosorption of Cr(VI) and CR on NCPPY is an endothermic, spontaneous, and entropy-driven process.
32084468	10	43	theme	simulated	1420:1428	arg1	conditions					1430:1439	simulated conditions	1420:1439	simulated conditions	1420:1439	Results indicated that NCPPY has great potential to remove Cr(VI) and CR binary mixture under simulated conditions.
32084468	4	44	theme	parameters	550:559	arg1	effect					530:535	The effect	526:535	The effect of different parameters like pH, temperature, contact time, adsorbent dosage and initial concentration of Chromium (Cr(VI)) and Congo Red (CR)	526:678	The effect of different parameters like pH, temperature, contact time, adsorbent dosage and initial concentration of Chromium (Cr(VI)) and Congo Red (CR) were optimised in batch mode.
32084468	7	45	from	biosorption	1044:1054	arg1	NCPPY					1076:1080	NCPPY	1076:1080	NCPPY	1076:1080	Thermodynamic data showed that the biosorption of Cr(VI) and CR on NCPPY is an endothermic, spontaneous, and entropy-driven process.
32084468	1	46	theme	cellulose	199:207	arg1	hydrolysis					185:194	acid hydrolysis	180:194	acid hydrolysis of cellulose	180:207	In this study, nanocellulose was synthesised by acid hydrolysis of cellulose and was coupled with polypyrrole supporting matrix.
32084468	5	47	theme	Response	710:717	arg1	tool					782:785	an optimization tool	766:785	an optimization tool for the efficient removal of Cr(VI) and CR	766:828	Response Surface Methodology (RSM) has been employed as an optimization tool for the efficient removal of Cr(VI) and CR and the maximum removal efficiency was found to be 80% and 85% respectively.
32084468	5	47	theme	Response	710:717	arg1	RSM					740:742	RSM	740:742	RSM	740:742	Response Surface Methodology (RSM) has been employed as an optimization tool for the efficient removal of Cr(VI) and CR and the maximum removal efficiency was found to be 80% and 85% respectively.
32084468	5	47	theme	Response	710:717	arg1	Methodology					727:737	Response Surface Methodology	710:737	Response Surface Methodology (RSM)	710:743	Response Surface Methodology (RSM) has been employed as an optimization tool for the efficient removal of Cr(VI) and CR and the maximum removal efficiency was found to be 80% and 85% respectively.
32084468	9	48	theme	ions	1263:1266	arg1	Effect					1241:1246	Effect	1241:1246	Effect of co-existing ions	1241:1266	Effect of co-existing ions were checked using several common salts and heavy metals.
32084468	5	49	theme	Cr	816:817	arg1	removal					805:811	the efficient removal	791:811	the efficient removal of Cr(VI) and CR	791:828	Response Surface Methodology (RSM) has been employed as an optimization tool for the efficient removal of Cr(VI) and CR and the maximum removal efficiency was found to be 80% and 85% respectively.
32084468	5	50	theme	Surface	719:725	arg1	tool					782:785	an optimization tool	766:785	an optimization tool for the efficient removal of Cr(VI) and CR	766:828	Response Surface Methodology (RSM) has been employed as an optimization tool for the efficient removal of Cr(VI) and CR and the maximum removal efficiency was found to be 80% and 85% respectively.
32084468	5	50	theme	Surface	719:725	arg1	RSM					740:742	RSM	740:742	RSM	740:742	Response Surface Methodology (RSM) has been employed as an optimization tool for the efficient removal of Cr(VI) and CR and the maximum removal efficiency was found to be 80% and 85% respectively.
32084468	5	50	theme	Surface	719:725	arg1	Methodology					727:737	Response Surface Methodology	710:737	Response Surface Methodology (RSM)	710:743	Response Surface Methodology (RSM) has been employed as an optimization tool for the efficient removal of Cr(VI) and CR and the maximum removal efficiency was found to be 80% and 85% respectively.
32084468	7	51	dep	Cr	1059:1060	arg1	VI					1062:1063	VI	1062:1063	VI	1062:1063	Thermodynamic data showed that the biosorption of Cr(VI) and CR on NCPPY is an endothermic, spontaneous, and entropy-driven process.
32084468	0	52	from	chromium	104:111	arg1	mixture					123:129	binary mixture	116:129	binary mixture	116:129	Surface modification of nanocellulose using polypyrrole for the adsorptive removal of Congo red dye and chromium in binary mixture.
32084468	9	53	theme	several	1287:1293	arg1	salts					1302:1306	several common salts	1287:1306	several common salts	1287:1306	Effect of co-existing ions were checked using several common salts and heavy metals.
32084468	9	54	theme	heavy	1312:1316	arg1	metals					1318:1323	heavy metals	1312:1323	heavy metals	1312:1323	Effect of co-existing ions were checked using several common salts and heavy metals.
32084468	0	55	mod	modification	8:19	arg3	Surface					0:6	Surface modification	0:19	Surface modification of nanocellulose	0:36	Surface modification of nanocellulose using polypyrrole for the adsorptive removal of Congo red dye and chromium in binary mixture.
32084468	0	55	mod	modification	8:19	arg1	nanocellulose					24:36	nanocellulose	24:36	nanocellulose	24:36	Surface modification of nanocellulose using polypyrrole for the adsorptive removal of Congo red dye and chromium in binary mixture.
32084468	6	56	dep	CR	981:982	arg1	VI					991:992	VI	991:992	VI	991:992	The Langmuir and Freundlich isotherm well fitted the equilibrium data for CR and Cr(VI) respectively.
32084468	3	57	theme	sized	440:444	arg1	structure					458:466	nano sized crystalline structure	435:466	nano sized crystalline structure	435:466	These analysis showed the conversion of cellulose to nano sized crystalline structure with excellent thermal stability and higher surface area.
32084468	5	58	dep	Cr	816:817	arg1	VI					819:820	VI	819:820	VI	819:820	Response Surface Methodology (RSM) has been employed as an optimization tool for the efficient removal of Cr(VI) and CR and the maximum removal efficiency was found to be 80% and 85% respectively.
32084468	4	59	theme	batch	698:702	arg1	mode					704:707	batch mode	698:707	batch mode	698:707	The effect of different parameters like pH, temperature, contact time, adsorbent dosage and initial concentration of Chromium (Cr(VI)) and Congo Red (CR) were optimised in batch mode.
32084468	5	60	theme	maximum	838:844	arg1	efficiency					854:863	the maximum removal efficiency	834:863	the maximum removal efficiency	834:863	Response Surface Methodology (RSM) has been employed as an optimization tool for the efficient removal of Cr(VI) and CR and the maximum removal efficiency was found to be 80% and 85% respectively.
32084468	5	60	theme	maximum	838:844	arg1	%					883:883	80%	881:883	80%	881:883	Response Surface Methodology (RSM) has been employed as an optimization tool for the efficient removal of Cr(VI) and CR and the maximum removal efficiency was found to be 80% and 85% respectively.
32084468	3	61	theme	crystalline	446:456	arg1	structure					458:466	nano sized crystalline structure	435:466	nano sized crystalline structure	435:466	These analysis showed the conversion of cellulose to nano sized crystalline structure with excellent thermal stability and higher surface area.
32084468	8	62	theme	kinetic	1157:1163	arg1	adsorption					1146:1155	The adsorption kinetic	1142:1163	The adsorption kinetic	1142:1163	The adsorption kinetic followed pseudo-second-order for Cr(VI) and intraparticle diffusion for CR.
32084468	3	63	theme	cellulose	422:430	arg1	conversion					408:417	the conversion	404:417	the conversion of cellulose to nano sized crystalline structure	404:466	These analysis showed the conversion of cellulose to nano sized crystalline structure with excellent thermal stability and higher surface area.
32084468	4	64	theme	adsorbent	597:605	arg1	dosage					607:612	adsorbent dosage	597:612	adsorbent dosage	597:612	The effect of different parameters like pH, temperature, contact time, adsorbent dosage and initial concentration of Chromium (Cr(VI)) and Congo Red (CR) were optimised in batch mode.
32084468	5	65	theme	optimization	769:780	arg1	tool					782:785	an optimization tool	766:785	an optimization tool for the efficient removal of Cr(VI) and CR	766:828	Response Surface Methodology (RSM) has been employed as an optimization tool for the efficient removal of Cr(VI) and CR and the maximum removal efficiency was found to be 80% and 85% respectively.
32084468	5	65	theme	optimization	769:780	arg1	Methodology					727:737	Response Surface Methodology	710:737	Response Surface Methodology (RSM)	710:743	Response Surface Methodology (RSM) has been employed as an optimization tool for the efficient removal of Cr(VI) and CR and the maximum removal efficiency was found to be 80% and 85% respectively.
32084468	0	66	theme	adsorptive	64:73	arg1	removal					75:81	the adsorptive removal	60:81	the adsorptive removal of Congo red dye	60:98	Surface modification of nanocellulose using polypyrrole for the adsorptive removal of Congo red dye and chromium in binary mixture.
32084468	7	67	theme	Thermodynamic	1009:1021	arg1	data					1023:1026	Thermodynamic data	1009:1026	Thermodynamic data	1009:1026	Thermodynamic data showed that the biosorption of Cr(VI) and CR on NCPPY is an endothermic, spontaneous, and entropy-driven process.
32084468	5	68	theme	efficient	795:803	arg1	removal					805:811	the efficient removal	791:811	the efficient removal of Cr(VI) and CR	791:828	Response Surface Methodology (RSM) has been employed as an optimization tool for the efficient removal of Cr(VI) and CR and the maximum removal efficiency was found to be 80% and 85% respectively.
32084468	4	69	theme	contact	583:589	arg1	time					591:594	contact time	583:594	contact time	583:594	The effect of different parameters like pH, temperature, contact time, adsorbent dosage and initial concentration of Chromium (Cr(VI)) and Congo Red (CR) were optimised in batch mode.
33479373	6	0	theme	recognition	1052:1062	arg1	lack					1044:1047	the lack	1040:1047	the lack of recognition of DAT3 by human T cells	1040:1087	In contrast with the lack of recognition of DAT3 by human T cells, DAT3, but not DAT1 or DAT2, activates Mincle.
33479373	1	1	theme	Mycobacterium	147:159	arg1	tuberculosis					161:172	Mycobacterium tuberculosis	147:172	Mycobacterium tuberculosis	147:172	The cell wall of Mycobacterium tuberculosis is composed of diverse glycolipids which potentially interact with the human immune system.
33479373	0	2	theme	cell	85:88	arg1	receptors					90:98	human T cell receptors	77:98	human T cell receptors	77:98	Synthetic mycobacterial diacyl trehaloses reveal differential recognition by human T cell receptors and the C-type lectin Mincle.
33479373	1	3	theme	tuberculosis	161:172	arg1	wall					139:142	The cell wall	130:142	The cell wall of Mycobacterium tuberculosis	130:172	The cell wall of Mycobacterium tuberculosis is composed of diverse glycolipids which potentially interact with the human immune system.
33479373	7	4	theme	immune	1349:1354	arg1	system					1356:1361	the immune system	1345:1361	the immune system	1345:1361	Thus, we show that the mycobacterial lipid DAT can be both an antigen for T cells and an agonist for the innate Mincle receptor, and that small chemical differences determine recognition by different parts of the immune system.
33479373	2	5	theme	pure	304:307	arg1	compounds					309:317	pure compounds	304:317	pure compounds	304:317	To overcome difficulties in obtaining pure compounds from bacterial extracts, we recently synthesized three forms of mycobacterial diacyltrehalose (DAT) that differ in their fatty acid composition, DAT1, DAT2, and DAT3.
33479373	4	6	theme	T	733:733	arg1	cells					735:739	T cells	733:739	T cells	733:739	DAT1- and DAT2-treated CD1b tetramers were recognized by T cells, but DAT3-treated CD1b tetramers were not.
33479373	3	7	theme	human	532:536	arg1	cells					540:544	human T cells	532:544	human T cells	532:544	To study the potential recognition of DATs by human T cells, we treated the lipid-binding antigen presenting molecule CD1b with synthetic DATs and looked for T cells that bound the complex.
33479373	4	8	theme	DAT2-treated	686:697	arg1	tetramers					704:712	DAT1- and DAT2-treated CD1b tetramers	676:712	DAT1- and DAT2-treated CD1b tetramers	676:712	DAT1- and DAT2-treated CD1b tetramers were recognized by T cells, but DAT3-treated CD1b tetramers were not.
33479373	0	9	theme	lectin	115:120	arg1	Mincle					122:127	the C-type lectin Mincle	104:127	the C-type lectin Mincle	104:127	Synthetic mycobacterial diacyl trehaloses reveal differential recognition by human T cell receptors and the C-type lectin Mincle.
33479373	7	10	theme	chemical	1280:1287	arg1	differences					1289:1299	small chemical differences	1274:1299	small chemical differences	1274:1299	Thus, we show that the mycobacterial lipid DAT can be both an antigen for T cells and an agonist for the innate Mincle receptor, and that small chemical differences determine recognition by different parts of the immune system.
33479373	5	11	theme	CD1b-DAT2	812:820	arg1	tetramers					822:830	CD1b-DAT2 tetramers	812:830	CD1b-DAT2 tetramers	812:830	A T cell line derived using CD1b-DAT2 tetramers showed that there is no cross-reactivity between DATs in an IFN-γ release assay, suggesting that the chemical structure of the fatty acid at the 3-position determines recognition by T cells.
33479373	0	12	theme	C-type	108:113	arg1	lectin					115:120	the C-type lectin	104:120	the C-type lectin Mincle	104:127	Synthetic mycobacterial diacyl trehaloses reveal differential recognition by human T cell receptors and the C-type lectin Mincle.
33479373	3	13	theme	DATs	524:527	arg1	recognition					509:519	the potential recognition	495:519	the potential recognition of DATs by human T cells	495:544	To study the potential recognition of DATs by human T cells, we treated the lipid-binding antigen presenting molecule CD1b with synthetic DATs and looked for T cells that bound the complex.
33479373	5	14	from	structure	942:950	arg1	3-position					977:986	the 3-position	973:986	the 3-position	973:986	A T cell line derived using CD1b-DAT2 tetramers showed that there is no cross-reactivity between DATs in an IFN-γ release assay, suggesting that the chemical structure of the fatty acid at the 3-position determines recognition by T cells.
33479373	0	15	theme	mycobacterial	10:22	arg1	trehaloses					31:40	Synthetic mycobacterial diacyl trehaloses	0:40	Synthetic mycobacterial diacyl trehaloses	0:40	Synthetic mycobacterial diacyl trehaloses reveal differential recognition by human T cell receptors and the C-type lectin Mincle.
33479373	7	16	theme	different	1326:1334	arg1	parts					1336:1340	different parts	1326:1340	different parts of the immune system	1326:1361	Thus, we show that the mycobacterial lipid DAT can be both an antigen for T cells and an agonist for the innate Mincle receptor, and that small chemical differences determine recognition by different parts of the immune system.
33479373	5	17	theme	IFN-γ	892:896	arg1	assay					906:910	an IFN-γ release assay	889:910	an IFN-γ release assay	889:910	A T cell line derived using CD1b-DAT2 tetramers showed that there is no cross-reactivity between DATs in an IFN-γ release assay, suggesting that the chemical structure of the fatty acid at the 3-position determines recognition by T cells.
33479373	7	18	theme	mycobacterial	1159:1171	arg1	antigen					1198:1204	an antigen	1195:1204	an antigen for T cells	1195:1216	Thus, we show that the mycobacterial lipid DAT can be both an antigen for T cells and an agonist for the innate Mincle receptor, and that small chemical differences determine recognition by different parts of the immune system.
33479373	7	18	theme	mycobacterial	1159:1171	arg1	DAT					1179:1181	the mycobacterial lipid DAT	1155:1181	the mycobacterial lipid DAT	1155:1181	Thus, we show that the mycobacterial lipid DAT can be both an antigen for T cells and an agonist for the innate Mincle receptor, and that small chemical differences determine recognition by different parts of the immune system.
33479373	0	19	theme	Synthetic	0:8	arg1	trehaloses					31:40	Synthetic mycobacterial diacyl trehaloses	0:40	Synthetic mycobacterial diacyl trehaloses	0:40	Synthetic mycobacterial diacyl trehaloses reveal differential recognition by human T cell receptors and the C-type lectin Mincle.
33479373	5	20	theme	T	1014:1014	arg1	cells					1016:1020	T cells	1014:1020	T cells	1014:1020	A T cell line derived using CD1b-DAT2 tetramers showed that there is no cross-reactivity between DATs in an IFN-γ release assay, suggesting that the chemical structure of the fatty acid at the 3-position determines recognition by T cells.
33479373	2	21	theme	bacterial	324:332	arg1	extracts					334:341	bacterial extracts	324:341	bacterial extracts	324:341	To overcome difficulties in obtaining pure compounds from bacterial extracts, we recently synthesized three forms of mycobacterial diacyltrehalose (DAT) that differ in their fatty acid composition, DAT1, DAT2, and DAT3.
33479373	2	22	theme	diacyltrehalose	397:411	arg1	forms					374:378	three forms	368:378	three forms of mycobacterial diacyltrehalose (DAT) that differ in their fatty acid composition, DAT1, DAT2, and DAT3	368:483	To overcome difficulties in obtaining pure compounds from bacterial extracts, we recently synthesized three forms of mycobacterial diacyltrehalose (DAT) that differ in their fatty acid composition, DAT1, DAT2, and DAT3.
33479373	2	23	theme	acid	446:449	arg1	composition					451:461	their fatty acid composition	434:461	their fatty acid composition	434:461	To overcome difficulties in obtaining pure compounds from bacterial extracts, we recently synthesized three forms of mycobacterial diacyltrehalose (DAT) that differ in their fatty acid composition, DAT1, DAT2, and DAT3.
33479373	7	24	theme	small	1274:1278	arg1	differences					1289:1299	small chemical differences	1274:1299	small chemical differences	1274:1299	Thus, we show that the mycobacterial lipid DAT can be both an antigen for T cells and an agonist for the innate Mincle receptor, and that small chemical differences determine recognition by different parts of the immune system.
33479373	3	25	theme	T	538:538	arg1	cells					540:544	human T cells	532:544	human T cells	532:544	To study the potential recognition of DATs by human T cells, we treated the lipid-binding antigen presenting molecule CD1b with synthetic DATs and looked for T cells that bound the complex.
33479373	5	26	theme	acid	965:968	arg1	structure					942:950	the chemical structure	929:950	the chemical structure of the fatty acid at the 3-position	929:986	A T cell line derived using CD1b-DAT2 tetramers showed that there is no cross-reactivity between DATs in an IFN-γ release assay, suggesting that the chemical structure of the fatty acid at the 3-position determines recognition by T cells.
33479373	5	27	theme	cell	788:791	arg1	line					793:796	A T cell line	784:796	A T cell line derived using CD1b-DAT2 tetramers	784:830	A T cell line derived using CD1b-DAT2 tetramers showed that there is no cross-reactivity between DATs in an IFN-γ release assay, suggesting that the chemical structure of the fatty acid at the 3-position determines recognition by T cells.
33479373	2	28	theme	fatty	440:444	arg1	composition					451:461	their fatty acid composition	434:461	their fatty acid composition	434:461	To overcome difficulties in obtaining pure compounds from bacterial extracts, we recently synthesized three forms of mycobacterial diacyltrehalose (DAT) that differ in their fatty acid composition, DAT1, DAT2, and DAT3.
33479373	5	29	theme	release	898:904	arg1	assay					906:910	an IFN-γ release assay	889:910	an IFN-γ release assay	889:910	A T cell line derived using CD1b-DAT2 tetramers showed that there is no cross-reactivity between DATs in an IFN-γ release assay, suggesting that the chemical structure of the fatty acid at the 3-position determines recognition by T cells.
33479373	6	30	theme	human	1075:1079	arg1	cells					1083:1087	human T cells	1075:1087	human T cells	1075:1087	In contrast with the lack of recognition of DAT3 by human T cells, DAT3, but not DAT1 or DAT2, activates Mincle.
33479373	3	31	theme	potential	499:507	arg1	recognition					509:519	the potential recognition	495:519	the potential recognition of DATs by human T cells	495:544	To study the potential recognition of DATs by human T cells, we treated the lipid-binding antigen presenting molecule CD1b with synthetic DATs and looked for T cells that bound the complex.
33479373	0	32	theme	differential	49:60	arg1	recognition					62:72	differential recognition	49:72	differential recognition by human T cell receptors	49:98	Synthetic mycobacterial diacyl trehaloses reveal differential recognition by human T cell receptors and the C-type lectin Mincle.
33479373	5	33	theme	fatty	959:963	arg1	acid					965:968	the fatty acid	955:968	the fatty acid at the 3-position	955:986	A T cell line derived using CD1b-DAT2 tetramers showed that there is no cross-reactivity between DATs in an IFN-γ release assay, suggesting that the chemical structure of the fatty acid at the 3-position determines recognition by T cells.
33479373	6	34	theme	DAT3	1067:1070	arg1	recognition					1052:1062	recognition	1052:1062	recognition of DAT3 by human T cells	1052:1087	In contrast with the lack of recognition of DAT3 by human T cells, DAT3, but not DAT1 or DAT2, activates Mincle.
33479373	3	35	theme	lipid-binding	562:574	arg1	CD1b					604:607	the lipid-binding antigen presenting molecule CD1b	558:607	the lipid-binding antigen presenting molecule CD1b	558:607	To study the potential recognition of DATs by human T cells, we treated the lipid-binding antigen presenting molecule CD1b with synthetic DATs and looked for T cells that bound the complex.
33479373	1	36	theme	diverse	189:195	arg1	glycolipids					197:207	diverse glycolipids	189:207	diverse glycolipids which potentially interact with the human immune system	189:263	The cell wall of Mycobacterium tuberculosis is composed of diverse glycolipids which potentially interact with the human immune system.
33479373	7	37	theme	T	1210:1210	arg1	cells					1212:1216	T cells	1210:1216	T cells	1210:1216	Thus, we show that the mycobacterial lipid DAT can be both an antigen for T cells and an agonist for the innate Mincle receptor, and that small chemical differences determine recognition by different parts of the immune system.
33479373	3	38	theme	synthetic	614:622	arg1	DATs					624:627	synthetic DATs	614:627	synthetic DATs	614:627	To study the potential recognition of DATs by human T cells, we treated the lipid-binding antigen presenting molecule CD1b with synthetic DATs and looked for T cells that bound the complex.
33479373	3	39	theme	antigen	576:582	arg1	CD1b					604:607	the lipid-binding antigen presenting molecule CD1b	558:607	the lipid-binding antigen presenting molecule CD1b	558:607	To study the potential recognition of DATs by human T cells, we treated the lipid-binding antigen presenting molecule CD1b with synthetic DATs and looked for T cells that bound the complex.
33479373	5	40	theme	T	786:786	arg1	line					793:796	A T cell line	784:796	A T cell line derived using CD1b-DAT2 tetramers	784:830	A T cell line derived using CD1b-DAT2 tetramers showed that there is no cross-reactivity between DATs in an IFN-γ release assay, suggesting that the chemical structure of the fatty acid at the 3-position determines recognition by T cells.
33479373	7	41	theme	lipid	1173:1177	arg1	antigen					1198:1204	an antigen	1195:1204	an antigen for T cells	1195:1216	Thus, we show that the mycobacterial lipid DAT can be both an antigen for T cells and an agonist for the innate Mincle receptor, and that small chemical differences determine recognition by different parts of the immune system.
33479373	7	41	theme	lipid	1173:1177	arg1	DAT					1179:1181	the mycobacterial lipid DAT	1155:1181	the mycobacterial lipid DAT	1155:1181	Thus, we show that the mycobacterial lipid DAT can be both an antigen for T cells and an agonist for the innate Mincle receptor, and that small chemical differences determine recognition by different parts of the immune system.
33479373	7	42	theme	Mincle	1248:1253	arg1	receptor					1255:1262	the innate Mincle receptor	1237:1262	the innate Mincle receptor	1237:1262	Thus, we show that the mycobacterial lipid DAT can be both an antigen for T cells and an agonist for the innate Mincle receptor, and that small chemical differences determine recognition by different parts of the immune system.
33479373	7	43	theme	innate	1241:1246	arg1	receptor					1255:1262	the innate Mincle receptor	1237:1262	the innate Mincle receptor	1237:1262	Thus, we show that the mycobacterial lipid DAT can be both an antigen for T cells and an agonist for the innate Mincle receptor, and that small chemical differences determine recognition by different parts of the immune system.
33479373	4	44	theme	DAT1-	676:680	arg1	tetramers					704:712	DAT1- and DAT2-treated CD1b tetramers	676:712	DAT1- and DAT2-treated CD1b tetramers	676:712	DAT1- and DAT2-treated CD1b tetramers were recognized by T cells, but DAT3-treated CD1b tetramers were not.
33479373	3	45	theme	molecule	595:602	arg1	CD1b					604:607	the lipid-binding antigen presenting molecule CD1b	558:607	the lipid-binding antigen presenting molecule CD1b	558:607	To study the potential recognition of DATs by human T cells, we treated the lipid-binding antigen presenting molecule CD1b with synthetic DATs and looked for T cells that bound the complex.
33479373	5	46	from	3-position	977:986	arg1	structure					942:950	the chemical structure	929:950	the chemical structure of the fatty acid at the 3-position	929:986	A T cell line derived using CD1b-DAT2 tetramers showed that there is no cross-reactivity between DATs in an IFN-γ release assay, suggesting that the chemical structure of the fatty acid at the 3-position determines recognition by T cells.
33479373	5	46	from	3-position	977:986	arg1	acid					965:968	the fatty acid	955:968	the fatty acid at the 3-position	955:986	A T cell line derived using CD1b-DAT2 tetramers showed that there is no cross-reactivity between DATs in an IFN-γ release assay, suggesting that the chemical structure of the fatty acid at the 3-position determines recognition by T cells.
33479373	4	47	theme	CD1b	759:762	arg1	tetramers					764:772	DAT3-treated CD1b tetramers	746:772	DAT3-treated CD1b tetramers	746:772	DAT1- and DAT2-treated CD1b tetramers were recognized by T cells, but DAT3-treated CD1b tetramers were not.
33479373	4	48	theme	CD1b	699:702	arg1	tetramers					704:712	DAT1- and DAT2-treated CD1b tetramers	676:712	DAT1- and DAT2-treated CD1b tetramers	676:712	DAT1- and DAT2-treated CD1b tetramers were recognized by T cells, but DAT3-treated CD1b tetramers were not.
33479373	6	49	theme	T	1081:1081	arg1	cells					1083:1087	human T cells	1075:1087	human T cells	1075:1087	In contrast with the lack of recognition of DAT3 by human T cells, DAT3, but not DAT1 or DAT2, activates Mincle.
33479373	5	50	theme	chemical	933:940	arg1	structure					942:950	the chemical structure	929:950	the chemical structure of the fatty acid at the 3-position	929:986	A T cell line derived using CD1b-DAT2 tetramers showed that there is no cross-reactivity between DATs in an IFN-γ release assay, suggesting that the chemical structure of the fatty acid at the 3-position determines recognition by T cells.
33479373	6	51	with	contrast	1026:1033	arg1	lack					1044:1047	the lack	1040:1047	the lack of recognition of DAT3 by human T cells	1040:1087	In contrast with the lack of recognition of DAT3 by human T cells, DAT3, but not DAT1 or DAT2, activates Mincle.
33479373	4	52	theme	DAT3-treated	746:757	arg1	tetramers					764:772	DAT3-treated CD1b tetramers	746:772	DAT3-treated CD1b tetramers	746:772	DAT1- and DAT2-treated CD1b tetramers were recognized by T cells, but DAT3-treated CD1b tetramers were not.
33479373	1	53	theme	cell	134:137	arg1	wall					139:142	The cell wall	130:142	The cell wall of Mycobacterium tuberculosis	130:172	The cell wall of Mycobacterium tuberculosis is composed of diverse glycolipids which potentially interact with the human immune system.
33479373	0	54	theme	T	83:83	arg1	receptors					90:98	human T cell receptors	77:98	human T cell receptors	77:98	Synthetic mycobacterial diacyl trehaloses reveal differential recognition by human T cell receptors and the C-type lectin Mincle.
33479373	3	55	theme	T	644:644	arg1	cells					646:650	T cells	644:650	T cells that bound the complex	644:673	To study the potential recognition of DATs by human T cells, we treated the lipid-binding antigen presenting molecule CD1b with synthetic DATs and looked for T cells that bound the complex.
33479373	3	56	theme	presenting	584:593	arg1	CD1b					604:607	the lipid-binding antigen presenting molecule CD1b	558:607	the lipid-binding antigen presenting molecule CD1b	558:607	To study the potential recognition of DATs by human T cells, we treated the lipid-binding antigen presenting molecule CD1b with synthetic DATs and looked for T cells that bound the complex.
33479373	2	57	theme	mycobacterial	383:395	arg1	DAT					414:416	DAT	414:416	DAT	414:416	To overcome difficulties in obtaining pure compounds from bacterial extracts, we recently synthesized three forms of mycobacterial diacyltrehalose (DAT) that differ in their fatty acid composition, DAT1, DAT2, and DAT3.
33479373	2	57	theme	mycobacterial	383:395	arg1	diacyltrehalose					397:411	mycobacterial diacyltrehalose	383:411	mycobacterial diacyltrehalose (DAT)	383:417	To overcome difficulties in obtaining pure compounds from bacterial extracts, we recently synthesized three forms of mycobacterial diacyltrehalose (DAT) that differ in their fatty acid composition, DAT1, DAT2, and DAT3.
33479373	1	58	theme	human	245:249	arg1	system					258:263	the human immune system	241:263	the human immune system	241:263	The cell wall of Mycobacterium tuberculosis is composed of diverse glycolipids which potentially interact with the human immune system.
33479373	0	59	theme	human	77:81	arg1	receptors					90:98	human T cell receptors	77:98	human T cell receptors	77:98	Synthetic mycobacterial diacyl trehaloses reveal differential recognition by human T cell receptors and the C-type lectin Mincle.
33479373	7	60	theme	system	1356:1361	arg1	parts					1336:1340	different parts	1326:1340	different parts of the immune system	1326:1361	Thus, we show that the mycobacterial lipid DAT can be both an antigen for T cells and an agonist for the innate Mincle receptor, and that small chemical differences determine recognition by different parts of the immune system.
33479373	0	61	theme	diacyl	24:29	arg1	trehaloses					31:40	Synthetic mycobacterial diacyl trehaloses	0:40	Synthetic mycobacterial diacyl trehaloses	0:40	Synthetic mycobacterial diacyl trehaloses reveal differential recognition by human T cell receptors and the C-type lectin Mincle.
33479373	1	62	theme	immune	251:256	arg1	system					258:263	the human immune system	241:263	the human immune system	241:263	The cell wall of Mycobacterium tuberculosis is composed of diverse glycolipids which potentially interact with the human immune system.
32950932	6	0	theme	increase/decrease	1249:1265	arg1	[AUCI					1267:1271	increase/decrease [AUCI	1249:1271	increase/decrease [AUCI	1249:1271	At visit two, after the blood draw, participants completed the Trier Social Stress Test (TSST); saliva samples were collected before and after to generate cortisol response curves (area under the curve with respect to ground [AUCG] and increase/decrease [AUCI]).
32950932	9	1	dep	0.80	1878:1881	arg1	<					1886:1886	p < 0.001	1884:1892	p < 0.001	1884:1892	Stimulated inflammatory cytokine production was strongly correlated across the two visits (2 weeks apart) after 4 hours of incubation (r = 0.80, p < 0.001) and after 24 hours (r = 0.80, p < 0.001).
32950932	6	2	theme	cortisol	1168:1175	arg1	curves					1186:1191	cortisol response curves	1168:1191	cortisol response curves (area under the curve with respect to ground [AUCG]	1168:1243	At visit two, after the blood draw, participants completed the Trier Social Stress Test (TSST); saliva samples were collected before and after to generate cortisol response curves (area under the curve with respect to ground [AUCG] and increase/decrease [AUCI]).
32950932	11	3	theme	cytokines	2299:2307	arg1	measurement					2273:2283	measurement	2273:2283	measurement of stimulated cytokines	2273:2307	Moreover, measurement of stimulated cytokines was highly stable across a two-week time period whether measured after 4 or 24 hours of incubation with LPS.
32950932	9	4	dep	correlated	1755:1764	arg1	0.80					1878:1881	r = 0.80	1874:1881	r = 0.80	1874:1881	Stimulated inflammatory cytokine production was strongly correlated across the two visits (2 weeks apart) after 4 hours of incubation (r = 0.80, p < 0.001) and after 24 hours (r = 0.80, p < 0.001).
32950932	9	4	dep	correlated	1755:1764	arg1	weeks					1791:1795	2 weeks	1789:1795	2 weeks apart	1789:1801	Stimulated inflammatory cytokine production was strongly correlated across the two visits (2 weeks apart) after 4 hours of incubation (r = 0.80, p < 0.001) and after 24 hours (r = 0.80, p < 0.001).
32950932	1	5	theme	stressors	272:280	arg1	types					263:267	different types	253:267	different types of stressors	253:280	BACKGROUND Empirical and theoretical evidence suggest that because of the co-evolution of the endocrine and immune response systems, different types of stressors may lead to similar levels of physiological activation.
32950932	4	6	theme	cytokine	737:744	arg1	levels					746:751	circulating inflammatory cytokine levels	712:751	circulating inflammatory cytokine levels	712:751	Blood was collected at each appointment to measure circulating inflammatory cytokine levels and stimulated inflammatory cytokine production after 4 and 24 hours of incubation with LPS.
32950932	6	7	dep	collected	1129:1137	arg1	before					1139:1144	before	1139:1144	before	1139:1144	At visit two, after the blood draw, participants completed the Trier Social Stress Test (TSST); saliva samples were collected before and after to generate cortisol response curves (area under the curve with respect to ground [AUCG] and increase/decrease [AUCI]).
32950932	6	7	dep	collected	1129:1137	arg1	generate					1159:1166	generate	1159:1166	to generate cortisol response curves (area under the curve with respect to ground [AUCG] and increase/decrease [AUCI])	1156:1273	At visit two, after the blood draw, participants completed the Trier Social Stress Test (TSST); saliva samples were collected before and after to generate cortisol response curves (area under the curve with respect to ground [AUCG] and increase/decrease [AUCI]).
32950932	6	8	theme	ground	1231:1236	arg1	[AUCG					1238:1242	ground [AUCG	1231:1242	ground [AUCG	1231:1242	At visit two, after the blood draw, participants completed the Trier Social Stress Test (TSST); saliva samples were collected before and after to generate cortisol response curves (area under the curve with respect to ground [AUCG] and increase/decrease [AUCI]).
32950932	3	9	theme	Healthy	569:575	arg1	N					597:597	N = 65	597:602	N = 65	597:602	METHODS Healthy middle-aged adults (N = 65) completed testing at two appointments, two weeks apart.
32950932	3	9	theme	Healthy	569:575	arg1	adults					589:594	METHODS Healthy middle-aged adults	561:594	METHODS Healthy middle-aged adults (N = 65)	561:603	METHODS Healthy middle-aged adults (N = 65) completed testing at two appointments, two weeks apart.
32950932	9	10	theme	=	1876:1876	arg1	0.80					1878:1881	r = 0.80	1874:1881	r = 0.80	1874:1881	Stimulated inflammatory cytokine production was strongly correlated across the two visits (2 weeks apart) after 4 hours of incubation (r = 0.80, p < 0.001) and after 24 hours (r = 0.80, p < 0.001).
32950932	9	11	theme	inflammatory	1709:1720	arg1	production					1731:1740	Stimulated inflammatory cytokine production	1698:1740	Stimulated inflammatory cytokine production	1698:1740	Stimulated inflammatory cytokine production was strongly correlated across the two visits (2 weeks apart) after 4 hours of incubation (r = 0.80, p < 0.001) and after 24 hours (r = 0.80, p < 0.001).
32950932	5	12	theme	composite	872:880	arg1	measure					882:888	A cumulative standardized composite measure	846:888	A cumulative standardized composite measure of inflammation	846:904	A cumulative standardized composite measure of inflammation was calculated using the cytokines interleukin-6 (IL-6), interleukin-1β (IL-1β), and interferon-γ (IFN-γ).
32950932	10	13	theme	human	2225:2229	arg1	responses					2252:2260	acute human physiological stress responses	2219:2260	acute human physiological stress responses	2219:2260	Within each visit, stimulated cytokine production after 4 hours was significantly correlated with stimulated inflammation at 24 hours (r = 0.93-0.94, p < 0.05) CONCLUSIONS: These results suggest that LPS-stimulated inflammatory cytokine production and the cortisol response to the TSST contain comparable information about acute human physiological stress responses.
32950932	6	14	dep	curves	1186:1191	arg1	area					1194:1197	area	1194:1197	area under the curve with respect to ground [AUCG	1194:1242	At visit two, after the blood draw, participants completed the Trier Social Stress Test (TSST); saliva samples were collected before and after to generate cortisol response curves (area under the curve with respect to ground [AUCG] and increase/decrease [AUCI]).
32950932	1	15	theme	BACKGROUND	120:129	arg1	evidence					157:164	BACKGROUND Empirical and theoretical evidence	120:164	BACKGROUND Empirical and theoretical evidence	120:164	BACKGROUND Empirical and theoretical evidence suggest that because of the co-evolution of the endocrine and immune response systems, different types of stressors may lead to similar levels of physiological activation.
32950932	10	16	theme	=	2033:2033	arg1	hours					2024:2028	24 hours	2021:2028	24 hours (r = 0.93-0.94, p < 0.05) CONCLUSIONS: These results suggest that LPS-stimulated inflammatory cytokine production and the cortisol response to the TSST contain comparable information about acute human physiological stress responses	2021:2260	Within each visit, stimulated cytokine production after 4 hours was significantly correlated with stimulated inflammation at 24 hours (r = 0.93-0.94, p < 0.05) CONCLUSIONS: These results suggest that LPS-stimulated inflammatory cytokine production and the cortisol response to the TSST contain comparable information about acute human physiological stress responses.
32950932	10	16	theme	=	2033:2033	arg1	r					2031:2031	r = 0.93-0.94	2031:2043	r = 0.93-0.94	2031:2043	Within each visit, stimulated cytokine production after 4 hours was significantly correlated with stimulated inflammation at 24 hours (r = 0.93-0.94, p < 0.05) CONCLUSIONS: These results suggest that LPS-stimulated inflammatory cytokine production and the cortisol response to the TSST contain comparable information about acute human physiological stress responses.
32950932	7	17	theme	cytokine	1334:1341	arg1	production					1343:1352	stimulated cytokine production	1323:1352	stimulated cytokine production at visit 2 after both 4 hours (B = 6.89; p = 0.007) and 24 hours (B = 7.50; p = 0.005) of incubation	1323:1453	RESULTS AUCG was significantly associated with stimulated cytokine production at visit 2 after both 4 hours (B = 6.89; p = 0.007) and 24 hours (B = 7.50; p = 0.005) of incubation, controlling for age, sex, and BMI.
32950932	10	18	theme	acute	2219:2223	arg1	responses					2252:2260	acute human physiological stress responses	2219:2260	acute human physiological stress responses	2219:2260	Within each visit, stimulated cytokine production after 4 hours was significantly correlated with stimulated inflammation at 24 hours (r = 0.93-0.94, p < 0.05) CONCLUSIONS: These results suggest that LPS-stimulated inflammatory cytokine production and the cortisol response to the TSST contain comparable information about acute human physiological stress responses.
32950932	0	19	theme	cortisol	62:69	arg1	curves					71:76	cortisol curves	62:76	cortisol curves in response to acute psychosocial stress	62:117	Ex vivo LPS-stimulated cytokine production is associated with cortisol curves in response to acute psychosocial stress.
32950932	5	20	theme	cumulative	848:857	arg1	measure					882:888	A cumulative standardized composite measure	846:888	A cumulative standardized composite measure of inflammation	846:904	A cumulative standardized composite measure of inflammation was calculated using the cytokines interleukin-6 (IL-6), interleukin-1β (IL-1β), and interferon-γ (IFN-γ).
32950932	4	21	theme	circulating	712:722	arg1	levels					746:751	circulating inflammatory cytokine levels	712:751	circulating inflammatory cytokine levels	712:751	Blood was collected at each appointment to measure circulating inflammatory cytokine levels and stimulated inflammatory cytokine production after 4 and 24 hours of incubation with LPS.
32950932	10	22	dep	CONCLUSIONS	2056:2066	arg1	suggest					2083:2089	suggest	2083:2089	suggest that LPS-stimulated inflammatory cytokine production and the cortisol response to the TSST contain comparable information about acute human physiological stress responses	2083:2260	Within each visit, stimulated cytokine production after 4 hours was significantly correlated with stimulated inflammation at 24 hours (r = 0.93-0.94, p < 0.05) CONCLUSIONS: These results suggest that LPS-stimulated inflammatory cytokine production and the cortisol response to the TSST contain comparable information about acute human physiological stress responses.
32950932	4	23	theme	cytokine	781:788	arg1	production					790:799	stimulated inflammatory cytokine production	757:799	stimulated inflammatory cytokine production	757:799	Blood was collected at each appointment to measure circulating inflammatory cytokine levels and stimulated inflammatory cytokine production after 4 and 24 hours of incubation with LPS.
32950932	7	24	theme	incubation	1444:1453	arg1	hours					1378:1382	4 hours	1376:1382	4 hours (B = 6.89; p = 0.007)	1376:1404	RESULTS AUCG was significantly associated with stimulated cytokine production at visit 2 after both 4 hours (B = 6.89; p = 0.007) and 24 hours (B = 7.50; p = 0.005) of incubation, controlling for age, sex, and BMI.
32950932	7	24	theme	incubation	1444:1453	arg1	hours					1413:1417	24 hours	1410:1417	24 hours (B = 7.50; p = 0.005) of incubation	1410:1453	RESULTS AUCG was significantly associated with stimulated cytokine production at visit 2 after both 4 hours (B = 6.89; p = 0.007) and 24 hours (B = 7.50; p = 0.005) of incubation, controlling for age, sex, and BMI.
32950932	2	25	theme	cytokine	540:547	arg1	production					549:558	inflammatory cytokine production	527:558	inflammatory cytokine production	527:558	The present analyses examined associations between two physiological stress responses: the cortisol response to an acute laboratory stressor and ex vivo lipopolysaccharide (LPS) stimulated inflammatory cytokine production.
32950932	0	26	theme	psychosocial	99:110	arg1	stress					112:117	acute psychosocial stress	93:117	acute psychosocial stress	93:117	Ex vivo LPS-stimulated cytokine production is associated with cortisol curves in response to acute psychosocial stress.
32950932	10	27	contain	contain	2182:2188	arg2	information					2201:2211	comparable information	2190:2211	comparable information about acute human physiological stress responses	2190:2260	Within each visit, stimulated cytokine production after 4 hours was significantly correlated with stimulated inflammation at 24 hours (r = 0.93-0.94, p < 0.05) CONCLUSIONS: These results suggest that LPS-stimulated inflammatory cytokine production and the cortisol response to the TSST contain comparable information about acute human physiological stress responses.
32950932	10	27	contain	contain	2182:2188	arg1	production					2133:2142	LPS-stimulated inflammatory cytokine production	2096:2142	LPS-stimulated inflammatory cytokine production	2096:2142	Within each visit, stimulated cytokine production after 4 hours was significantly correlated with stimulated inflammation at 24 hours (r = 0.93-0.94, p < 0.05) CONCLUSIONS: These results suggest that LPS-stimulated inflammatory cytokine production and the cortisol response to the TSST contain comparable information about acute human physiological stress responses.
32950932	10	27	contain	contain	2182:2188	arg1	response					2161:2168	the cortisol response	2148:2168	the cortisol response to the TSST	2148:2180	Within each visit, stimulated cytokine production after 4 hours was significantly correlated with stimulated inflammation at 24 hours (r = 0.93-0.94, p < 0.05) CONCLUSIONS: These results suggest that LPS-stimulated inflammatory cytokine production and the cortisol response to the TSST contain comparable information about acute human physiological stress responses.
32950932	6	28	theme	Trier	1076:1080	arg1	TSST					1102:1105	TSST	1102:1105	TSST	1102:1105	At visit two, after the blood draw, participants completed the Trier Social Stress Test (TSST); saliva samples were collected before and after to generate cortisol response curves (area under the curve with respect to ground [AUCG] and increase/decrease [AUCI]).
32950932	6	28	theme	Trier	1076:1080	arg1	Test					1096:1099	the Trier Social Stress Test	1072:1099	the Trier Social Stress Test (TSST)	1072:1106	At visit two, after the blood draw, participants completed the Trier Social Stress Test (TSST); saliva samples were collected before and after to generate cortisol response curves (area under the curve with respect to ground [AUCG] and increase/decrease [AUCI]).
32950932	10	29	theme	inflammatory	2111:2122	arg1	production					2133:2142	LPS-stimulated inflammatory cytokine production	2096:2142	LPS-stimulated inflammatory cytokine production	2096:2142	Within each visit, stimulated cytokine production after 4 hours was significantly correlated with stimulated inflammation at 24 hours (r = 0.93-0.94, p < 0.05) CONCLUSIONS: These results suggest that LPS-stimulated inflammatory cytokine production and the cortisol response to the TSST contain comparable information about acute human physiological stress responses.
32950932	8	30	dep	hours	1585:1589	arg1	=					1604:1604	=	1604:1604	=	1604:1604	AUCI was also significantly associated with stimulated cytokine production at visit 2 after 4 hours (B = 6.28; p = 0.004) and 24 hours (B = 6.16; p = 0.007) of incubation, controlling for age, sex, and BMI.
32950932	4	31	theme	inflammatory	768:779	arg1	production					790:799	stimulated inflammatory cytokine production	757:799	stimulated inflammatory cytokine production	757:799	Blood was collected at each appointment to measure circulating inflammatory cytokine levels and stimulated inflammatory cytokine production after 4 and 24 hours of incubation with LPS.
32950932	2	32	theme	stress	407:412	arg1	responses					414:422	two physiological stress responses	389:422	two physiological stress responses	389:422	The present analyses examined associations between two physiological stress responses: the cortisol response to an acute laboratory stressor and ex vivo lipopolysaccharide (LPS) stimulated inflammatory cytokine production.
32950932	0	33	theme	Ex	0:1	arg1	production					32:41	Ex vivo LPS-stimulated cytokine production	0:41	Ex vivo LPS-stimulated cytokine production	0:41	Ex vivo LPS-stimulated cytokine production is associated with cortisol curves in response to acute psychosocial stress.
32950932	2	34	theme	acute	453:457	arg1	stressor					470:477	an acute laboratory stressor	450:477	an acute laboratory stressor	450:477	The present analyses examined associations between two physiological stress responses: the cortisol response to an acute laboratory stressor and ex vivo lipopolysaccharide (LPS) stimulated inflammatory cytokine production.
32950932	11	35	theme	time	2345:2348	arg1	period					2350:2355	a two-week time period	2334:2355	a two-week time period	2334:2355	Moreover, measurement of stimulated cytokines was highly stable across a two-week time period whether measured after 4 or 24 hours of incubation with LPS.
32950932	10	36	theme	stimulated	1915:1924	arg1	production					1935:1944	stimulated cytokine production	1915:1944	stimulated cytokine production after 4 hours	1915:1958	Within each visit, stimulated cytokine production after 4 hours was significantly correlated with stimulated inflammation at 24 hours (r = 0.93-0.94, p < 0.05) CONCLUSIONS: These results suggest that LPS-stimulated inflammatory cytokine production and the cortisol response to the TSST contain comparable information about acute human physiological stress responses.
32950932	0	37	theme	LPS-stimulated	8:21	arg1	production					32:41	Ex vivo LPS-stimulated cytokine production	0:41	Ex vivo LPS-stimulated cytokine production	0:41	Ex vivo LPS-stimulated cytokine production is associated with cortisol curves in response to acute psychosocial stress.
32950932	7	38	dep	hours	1413:1417	arg1	=					1432:1432	=	1432:1432	=	1432:1432	RESULTS AUCG was significantly associated with stimulated cytokine production at visit 2 after both 4 hours (B = 6.89; p = 0.007) and 24 hours (B = 7.50; p = 0.005) of incubation, controlling for age, sex, and BMI.
32950932	10	39	theme	LPS-stimulated	2096:2109	arg1	production					2133:2142	LPS-stimulated inflammatory cytokine production	2096:2142	LPS-stimulated inflammatory cytokine production	2096:2142	Within each visit, stimulated cytokine production after 4 hours was significantly correlated with stimulated inflammation at 24 hours (r = 0.93-0.94, p < 0.05) CONCLUSIONS: These results suggest that LPS-stimulated inflammatory cytokine production and the cortisol response to the TSST contain comparable information about acute human physiological stress responses.
32950932	7	40	theme	RESULTS	1276:1282	arg1	AUCG					1284:1287	RESULTS AUCG	1276:1287	RESULTS AUCG	1276:1287	RESULTS AUCG was significantly associated with stimulated cytokine production at visit 2 after both 4 hours (B = 6.89; p = 0.007) and 24 hours (B = 7.50; p = 0.005) of incubation, controlling for age, sex, and BMI.
32950932	2	41	theme	cortisol	429:436	arg1	response					438:445	the cortisol response	425:445	the cortisol response to an acute laboratory stressor and ex vivo lipopolysaccharide (LPS)	425:514	The present analyses examined associations between two physiological stress responses: the cortisol response to an acute laboratory stressor and ex vivo lipopolysaccharide (LPS) stimulated inflammatory cytokine production.
32950932	2	42	theme	ex	483:484	arg1	LPS					511:513	LPS	511:513	LPS	511:513	The present analyses examined associations between two physiological stress responses: the cortisol response to an acute laboratory stressor and ex vivo lipopolysaccharide (LPS) stimulated inflammatory cytokine production.
32950932	2	42	theme	ex	483:484	arg1	lipopolysaccharide					491:508	ex vivo lipopolysaccharide	483:508	ex vivo lipopolysaccharide (LPS)	483:514	The present analyses examined associations between two physiological stress responses: the cortisol response to an acute laboratory stressor and ex vivo lipopolysaccharide (LPS) stimulated inflammatory cytokine production.
32950932	9	43	theme	=	1835:1835	arg1	incubation					1821:1830	incubation	1821:1830	incubation (r = 0.80, p < 0.001)	1821:1852	Stimulated inflammatory cytokine production was strongly correlated across the two visits (2 weeks apart) after 4 hours of incubation (r = 0.80, p < 0.001) and after 24 hours (r = 0.80, p < 0.001).
32950932	9	43	theme	=	1835:1835	arg1	0.80					1837:1840	r = 0.80	1833:1840	r = 0.80	1833:1840	Stimulated inflammatory cytokine production was strongly correlated across the two visits (2 weeks apart) after 4 hours of incubation (r = 0.80, p < 0.001) and after 24 hours (r = 0.80, p < 0.001).
32950932	1	44	theme	physiological	312:324	arg1	activation					326:335	physiological activation	312:335	physiological activation	312:335	BACKGROUND Empirical and theoretical evidence suggest that because of the co-evolution of the endocrine and immune response systems, different types of stressors may lead to similar levels of physiological activation.
32950932	6	45	theme	saliva	1109:1114	arg1	samples					1116:1122	saliva samples	1109:1122	saliva samples	1109:1122	At visit two, after the blood draw, participants completed the Trier Social Stress Test (TSST); saliva samples were collected before and after to generate cortisol response curves (area under the curve with respect to ground [AUCG] and increase/decrease [AUCI]).
32950932	6	46	theme	response	1177:1184	arg1	curves					1186:1191	cortisol response curves	1168:1191	cortisol response curves (area under the curve with respect to ground [AUCG]	1168:1243	At visit two, after the blood draw, participants completed the Trier Social Stress Test (TSST); saliva samples were collected before and after to generate cortisol response curves (area under the curve with respect to ground [AUCG] and increase/decrease [AUCI]).
32950932	1	47	theme	immune	228:233	arg1	systems					244:250	the endocrine and immune response systems	210:250	the endocrine and immune response systems	210:250	BACKGROUND Empirical and theoretical evidence suggest that because of the co-evolution of the endocrine and immune response systems, different types of stressors may lead to similar levels of physiological activation.
32950932	11	48	theme	stimulated	2288:2297	arg1	cytokines					2299:2307	stimulated cytokines	2288:2307	stimulated cytokines	2288:2307	Moreover, measurement of stimulated cytokines was highly stable across a two-week time period whether measured after 4 or 24 hours of incubation with LPS.
32950932	9	49	theme	<	1845:1845	arg1	p					1843:1843	p < 0.001	1843:1851	p < 0.001	1843:1851	Stimulated inflammatory cytokine production was strongly correlated across the two visits (2 weeks apart) after 4 hours of incubation (r = 0.80, p < 0.001) and after 24 hours (r = 0.80, p < 0.001).
32950932	1	50	theme	systems	244:250	arg1	co-evolution					194:205	the co-evolution	190:205	the co-evolution of the endocrine and immune response systems	190:250	BACKGROUND Empirical and theoretical evidence suggest that because of the co-evolution of the endocrine and immune response systems, different types of stressors may lead to similar levels of physiological activation.
32950932	10	51	dep	r	2031:2031	arg1	<					2048:2048	p < 0.05	2046:2053	p < 0.05	2046:2053	Within each visit, stimulated cytokine production after 4 hours was significantly correlated with stimulated inflammation at 24 hours (r = 0.93-0.94, p < 0.05) CONCLUSIONS: These results suggest that LPS-stimulated inflammatory cytokine production and the cortisol response to the TSST contain comparable information about acute human physiological stress responses.
32950932	0	52	dep	Ex	0:1	arg1	vivo					3:6	vivo	3:6	vivo	3:6	Ex vivo LPS-stimulated cytokine production is associated with cortisol curves in response to acute psychosocial stress.
32950932	8	53	theme	incubation	1651:1660	arg1	hours					1585:1589	4 hours	1583:1589	4 hours (B = 6.28; p = 0.004)	1583:1611	AUCI was also significantly associated with stimulated cytokine production at visit 2 after 4 hours (B = 6.28; p = 0.004) and 24 hours (B = 6.16; p = 0.007) of incubation, controlling for age, sex, and BMI.
32950932	8	53	theme	incubation	1651:1660	arg1	hours					1620:1624	24 hours	1617:1624	24 hours (B = 6.16; p = 0.007)	1617:1646	AUCI was also significantly associated with stimulated cytokine production at visit 2 after 4 hours (B = 6.28; p = 0.004) and 24 hours (B = 6.16; p = 0.007) of incubation, controlling for age, sex, and BMI.
32950932	1	54	theme	different	253:261	arg1	types					263:267	different types	253:267	different types of stressors	253:280	BACKGROUND Empirical and theoretical evidence suggest that because of the co-evolution of the endocrine and immune response systems, different types of stressors may lead to similar levels of physiological activation.
32950932	6	55	theme	Stress	1089:1094	arg1	TSST					1102:1105	TSST	1102:1105	TSST	1102:1105	At visit two, after the blood draw, participants completed the Trier Social Stress Test (TSST); saliva samples were collected before and after to generate cortisol response curves (area under the curve with respect to ground [AUCG] and increase/decrease [AUCI]).
32950932	6	55	theme	Stress	1089:1094	arg1	Test					1096:1099	the Trier Social Stress Test	1072:1099	the Trier Social Stress Test (TSST)	1072:1106	At visit two, after the blood draw, participants completed the Trier Social Stress Test (TSST); saliva samples were collected before and after to generate cortisol response curves (area under the curve with respect to ground [AUCG] and increase/decrease [AUCI]).
32950932	8	56	dep	=	1604:1604	arg1	6.28					1596:1599	6.28	1596:1599	6.28	1596:1599	AUCI was also significantly associated with stimulated cytokine production at visit 2 after 4 hours (B = 6.28; p = 0.004) and 24 hours (B = 6.16; p = 0.007) of incubation, controlling for age, sex, and BMI.
32950932	3	57	theme	middle-aged	577:587	arg1	N					597:597	N = 65	597:602	N = 65	597:602	METHODS Healthy middle-aged adults (N = 65) completed testing at two appointments, two weeks apart.
32950932	3	57	theme	middle-aged	577:587	arg1	adults					589:594	METHODS Healthy middle-aged adults	561:594	METHODS Healthy middle-aged adults (N = 65)	561:603	METHODS Healthy middle-aged adults (N = 65) completed testing at two appointments, two weeks apart.
32950932	3	58	theme	METHODS	561:567	arg1	N					597:597	N = 65	597:602	N = 65	597:602	METHODS Healthy middle-aged adults (N = 65) completed testing at two appointments, two weeks apart.
32950932	3	58	theme	METHODS	561:567	arg1	adults					589:594	METHODS Healthy middle-aged adults	561:594	METHODS Healthy middle-aged adults (N = 65)	561:603	METHODS Healthy middle-aged adults (N = 65) completed testing at two appointments, two weeks apart.
32950932	5	59	theme	inflammation	893:904	arg1	measure					882:888	A cumulative standardized composite measure	846:888	A cumulative standardized composite measure of inflammation	846:904	A cumulative standardized composite measure of inflammation was calculated using the cytokines interleukin-6 (IL-6), interleukin-1β (IL-1β), and interferon-γ (IFN-γ).
32950932	10	60	dep	hours	2024:2028	arg1	CONCLUSIONS					2056:2066	CONCLUSIONS: These results suggest that LPS-stimulated inflammatory cytokine production and the cortisol response to the TSST contain comparable information about acute human physiological stress responses	2056:2260	24 hours (r = 0.93-0.94, p < 0.05) CONCLUSIONS: These results suggest that LPS-stimulated inflammatory cytokine production and the cortisol response to the TSST contain comparable information about acute human physiological stress responses	2021:2260	Within each visit, stimulated cytokine production after 4 hours was significantly correlated with stimulated inflammation at 24 hours (r = 0.93-0.94, p < 0.05) CONCLUSIONS: These results suggest that LPS-stimulated inflammatory cytokine production and the cortisol response to the TSST contain comparable information about acute human physiological stress responses.
32950932	10	61	theme	stimulated	1994:2003	arg1	inflammation					2005:2016	stimulated inflammation	1994:2016	stimulated inflammation	1994:2016	Within each visit, stimulated cytokine production after 4 hours was significantly correlated with stimulated inflammation at 24 hours (r = 0.93-0.94, p < 0.05) CONCLUSIONS: These results suggest that LPS-stimulated inflammatory cytokine production and the cortisol response to the TSST contain comparable information about acute human physiological stress responses.
32950932	8	62	dep	=	1639:1639	arg1	6.16					1631:1634	6.16	1631:1634	6.16	1631:1634	AUCI was also significantly associated with stimulated cytokine production at visit 2 after 4 hours (B = 6.28; p = 0.004) and 24 hours (B = 6.16; p = 0.007) of incubation, controlling for age, sex, and BMI.
32950932	9	63	theme	r	1874:1874	arg1	0.80					1878:1881	r = 0.80	1874:1881	r = 0.80	1874:1881	Stimulated inflammatory cytokine production was strongly correlated across the two visits (2 weeks apart) after 4 hours of incubation (r = 0.80, p < 0.001) and after 24 hours (r = 0.80, p < 0.001).
32950932	3	64	theme	=	599:599	arg1	N					597:597	N = 65	597:602	N = 65	597:602	METHODS Healthy middle-aged adults (N = 65) completed testing at two appointments, two weeks apart.
32950932	3	64	theme	=	599:599	arg1	adults					589:594	METHODS Healthy middle-aged adults	561:594	METHODS Healthy middle-aged adults (N = 65)	561:603	METHODS Healthy middle-aged adults (N = 65) completed testing at two appointments, two weeks apart.
32950932	9	65	theme	Stimulated	1698:1707	arg1	production					1731:1740	Stimulated inflammatory cytokine production	1698:1740	Stimulated inflammatory cytokine production	1698:1740	Stimulated inflammatory cytokine production was strongly correlated across the two visits (2 weeks apart) after 4 hours of incubation (r = 0.80, p < 0.001) and after 24 hours (r = 0.80, p < 0.001).
32950932	10	66	theme	cortisol	2152:2159	arg1	response					2161:2168	the cortisol response	2148:2168	the cortisol response to the TSST	2148:2180	Within each visit, stimulated cytokine production after 4 hours was significantly correlated with stimulated inflammation at 24 hours (r = 0.93-0.94, p < 0.05) CONCLUSIONS: These results suggest that LPS-stimulated inflammatory cytokine production and the cortisol response to the TSST contain comparable information about acute human physiological stress responses.
32950932	5	67	theme	standardized	859:870	arg1	measure					882:888	A cumulative standardized composite measure	846:888	A cumulative standardized composite measure of inflammation	846:904	A cumulative standardized composite measure of inflammation was calculated using the cytokines interleukin-6 (IL-6), interleukin-1β (IL-1β), and interferon-γ (IFN-γ).
32950932	11	68	theme	incubation	2397:2406	arg1	hours					2388:2392	4 or 24 hours	2380:2392	4 or 24 hours of incubation with LPS	2380:2415	Moreover, measurement of stimulated cytokines was highly stable across a two-week time period whether measured after 4 or 24 hours of incubation with LPS.
32950932	9	69	theme	cytokine	1722:1729	arg1	production					1731:1740	Stimulated inflammatory cytokine production	1698:1740	Stimulated inflammatory cytokine production	1698:1740	Stimulated inflammatory cytokine production was strongly correlated across the two visits (2 weeks apart) after 4 hours of incubation (r = 0.80, p < 0.001) and after 24 hours (r = 0.80, p < 0.001).
32950932	10	70	theme	comparable	2190:2199	arg1	information					2201:2211	comparable information	2190:2211	comparable information about acute human physiological stress responses	2190:2260	Within each visit, stimulated cytokine production after 4 hours was significantly correlated with stimulated inflammation at 24 hours (r = 0.93-0.94, p < 0.05) CONCLUSIONS: These results suggest that LPS-stimulated inflammatory cytokine production and the cortisol response to the TSST contain comparable information about acute human physiological stress responses.
32950932	9	71	theme	p	1884:1884	arg1	<					1886:1886	p < 0.001	1884:1892	p < 0.001	1884:1892	Stimulated inflammatory cytokine production was strongly correlated across the two visits (2 weeks apart) after 4 hours of incubation (r = 0.80, p < 0.001) and after 24 hours (r = 0.80, p < 0.001).
32950932	1	72	theme	endocrine	214:222	arg1	systems					244:250	the endocrine and immune response systems	210:250	the endocrine and immune response systems	210:250	BACKGROUND Empirical and theoretical evidence suggest that because of the co-evolution of the endocrine and immune response systems, different types of stressors may lead to similar levels of physiological activation.
32950932	0	73	from	curves	71:76	arg1	response					81:88	response	81:88	response to acute psychosocial stress	81:117	Ex vivo LPS-stimulated cytokine production is associated with cortisol curves in response to acute psychosocial stress.
32950932	4	74	theme	inflammatory	724:735	arg1	levels					746:751	circulating inflammatory cytokine levels	712:751	circulating inflammatory cytokine levels	712:751	Blood was collected at each appointment to measure circulating inflammatory cytokine levels and stimulated inflammatory cytokine production after 4 and 24 hours of incubation with LPS.
32950932	1	75	theme	Empirical	131:139	arg1	evidence					157:164	BACKGROUND Empirical and theoretical evidence	120:164	BACKGROUND Empirical and theoretical evidence	120:164	BACKGROUND Empirical and theoretical evidence suggest that because of the co-evolution of the endocrine and immune response systems, different types of stressors may lead to similar levels of physiological activation.
32950932	10	76	theme	physiological	2231:2243	arg1	responses					2252:2260	acute human physiological stress responses	2219:2260	acute human physiological stress responses	2219:2260	Within each visit, stimulated cytokine production after 4 hours was significantly correlated with stimulated inflammation at 24 hours (r = 0.93-0.94, p < 0.05) CONCLUSIONS: These results suggest that LPS-stimulated inflammatory cytokine production and the cortisol response to the TSST contain comparable information about acute human physiological stress responses.
32950932	1	77	theme	theoretical	145:155	arg1	evidence					157:164	BACKGROUND Empirical and theoretical evidence	120:164	BACKGROUND Empirical and theoretical evidence	120:164	BACKGROUND Empirical and theoretical evidence suggest that because of the co-evolution of the endocrine and immune response systems, different types of stressors may lead to similar levels of physiological activation.
32950932	7	78	theme	stimulated	1323:1332	arg1	production					1343:1352	stimulated cytokine production	1323:1352	stimulated cytokine production at visit 2 after both 4 hours (B = 6.89; p = 0.007) and 24 hours (B = 7.50; p = 0.005) of incubation	1323:1453	RESULTS AUCG was significantly associated with stimulated cytokine production at visit 2 after both 4 hours (B = 6.89; p = 0.007) and 24 hours (B = 7.50; p = 0.005) of incubation, controlling for age, sex, and BMI.
32950932	2	79	dep	ex	483:484	arg1	vivo					486:489	vivo	486:489	vivo	486:489	The present analyses examined associations between two physiological stress responses: the cortisol response to an acute laboratory stressor and ex vivo lipopolysaccharide (LPS) stimulated inflammatory cytokine production.
32950932	7	80	dep	hours	1378:1382	arg1	=					1397:1397	=	1397:1397	=	1397:1397	RESULTS AUCG was significantly associated with stimulated cytokine production at visit 2 after both 4 hours (B = 6.89; p = 0.007) and 24 hours (B = 7.50; p = 0.005) of incubation, controlling for age, sex, and BMI.
32950932	10	81	theme	p	2046:2046	arg1	<					2048:2048	p < 0.05	2046:2053	p < 0.05	2046:2053	Within each visit, stimulated cytokine production after 4 hours was significantly correlated with stimulated inflammation at 24 hours (r = 0.93-0.94, p < 0.05) CONCLUSIONS: These results suggest that LPS-stimulated inflammatory cytokine production and the cortisol response to the TSST contain comparable information about acute human physiological stress responses.
32950932	11	82	with	incubation	2397:2406	arg1	LPS					2413:2415	LPS	2413:2415	LPS	2413:2415	Moreover, measurement of stimulated cytokines was highly stable across a two-week time period whether measured after 4 or 24 hours of incubation with LPS.
32950932	10	83	theme	stress	2245:2250	arg1	responses					2252:2260	acute human physiological stress responses	2219:2260	acute human physiological stress responses	2219:2260	Within each visit, stimulated cytokine production after 4 hours was significantly correlated with stimulated inflammation at 24 hours (r = 0.93-0.94, p < 0.05) CONCLUSIONS: These results suggest that LPS-stimulated inflammatory cytokine production and the cortisol response to the TSST contain comparable information about acute human physiological stress responses.
32950932	4	84	with	incubation	825:834	arg1	LPS					841:843	LPS	841:843	LPS	841:843	Blood was collected at each appointment to measure circulating inflammatory cytokine levels and stimulated inflammatory cytokine production after 4 and 24 hours of incubation with LPS.
32950932	0	85	theme	acute	93:97	arg1	stress					112:117	acute psychosocial stress	93:117	acute psychosocial stress	93:117	Ex vivo LPS-stimulated cytokine production is associated with cortisol curves in response to acute psychosocial stress.
32950932	9	86	dep	0.80	1837:1840	arg1	p					1843:1843	p < 0.001	1843:1851	p < 0.001	1843:1851	Stimulated inflammatory cytokine production was strongly correlated across the two visits (2 weeks apart) after 4 hours of incubation (r = 0.80, p < 0.001) and after 24 hours (r = 0.80, p < 0.001).
32950932	6	87	dep	blood	1037:1041	arg1	draw					1043:1046	draw	1043:1046	draw	1043:1046	At visit two, after the blood draw, participants completed the Trier Social Stress Test (TSST); saliva samples were collected before and after to generate cortisol response curves (area under the curve with respect to ground [AUCG] and increase/decrease [AUCI]).
32950932	6	88	theme	Social	1082:1087	arg1	TSST					1102:1105	TSST	1102:1105	TSST	1102:1105	At visit two, after the blood draw, participants completed the Trier Social Stress Test (TSST); saliva samples were collected before and after to generate cortisol response curves (area under the curve with respect to ground [AUCG] and increase/decrease [AUCI]).
32950932	6	88	theme	Social	1082:1087	arg1	Test					1096:1099	the Trier Social Stress Test	1072:1099	the Trier Social Stress Test (TSST)	1072:1106	At visit two, after the blood draw, participants completed the Trier Social Stress Test (TSST); saliva samples were collected before and after to generate cortisol response curves (area under the curve with respect to ground [AUCG] and increase/decrease [AUCI]).
32950932	2	89	theme	inflammatory	527:538	arg1	production					549:558	inflammatory cytokine production	527:558	inflammatory cytokine production	527:558	The present analyses examined associations between two physiological stress responses: the cortisol response to an acute laboratory stressor and ex vivo lipopolysaccharide (LPS) stimulated inflammatory cytokine production.
32950932	1	90	theme	response	235:242	arg1	systems					244:250	the endocrine and immune response systems	210:250	the endocrine and immune response systems	210:250	BACKGROUND Empirical and theoretical evidence suggest that because of the co-evolution of the endocrine and immune response systems, different types of stressors may lead to similar levels of physiological activation.
32950932	2	91	theme	laboratory	459:468	arg1	stressor					470:477	an acute laboratory stressor	450:477	an acute laboratory stressor	450:477	The present analyses examined associations between two physiological stress responses: the cortisol response to an acute laboratory stressor and ex vivo lipopolysaccharide (LPS) stimulated inflammatory cytokine production.
32950932	11	92	theme	two-week	2336:2343	arg1	period					2350:2355	a two-week time period	2334:2355	a two-week time period	2334:2355	Moreover, measurement of stimulated cytokines was highly stable across a two-week time period whether measured after 4 or 24 hours of incubation with LPS.
32950932	10	93	theme	cytokine	1926:1933	arg1	production					1935:1944	stimulated cytokine production	1915:1944	stimulated cytokine production after 4 hours	1915:1958	Within each visit, stimulated cytokine production after 4 hours was significantly correlated with stimulated inflammation at 24 hours (r = 0.93-0.94, p < 0.05) CONCLUSIONS: These results suggest that LPS-stimulated inflammatory cytokine production and the cortisol response to the TSST contain comparable information about acute human physiological stress responses.
32950932	4	94	theme	stimulated	757:766	arg1	production					790:799	stimulated inflammatory cytokine production	757:799	stimulated inflammatory cytokine production	757:799	Blood was collected at each appointment to measure circulating inflammatory cytokine levels and stimulated inflammatory cytokine production after 4 and 24 hours of incubation with LPS.
32950932	2	95	theme	physiological	393:405	arg1	responses					414:422	two physiological stress responses	389:422	two physiological stress responses	389:422	The present analyses examined associations between two physiological stress responses: the cortisol response to an acute laboratory stressor and ex vivo lipopolysaccharide (LPS) stimulated inflammatory cytokine production.
32950932	0	96	theme	cytokine	23:30	arg1	production					32:41	Ex vivo LPS-stimulated cytokine production	0:41	Ex vivo LPS-stimulated cytokine production	0:41	Ex vivo LPS-stimulated cytokine production is associated with cortisol curves in response to acute psychosocial stress.
32950932	7	97	from	visit	1357:1361	arg1	production					1343:1352	stimulated cytokine production	1323:1352	stimulated cytokine production at visit 2 after both 4 hours (B = 6.89; p = 0.007) and 24 hours (B = 7.50; p = 0.005) of incubation	1323:1453	RESULTS AUCG was significantly associated with stimulated cytokine production at visit 2 after both 4 hours (B = 6.89; p = 0.007) and 24 hours (B = 7.50; p = 0.005) of incubation, controlling for age, sex, and BMI.
32950932	9	98	theme	incubation	1821:1830	arg1	hours					1812:1816	4 hours	1810:1816	4 hours of incubation (r = 0.80, p < 0.001)	1810:1852	Stimulated inflammatory cytokine production was strongly correlated across the two visits (2 weeks apart) after 4 hours of incubation (r = 0.80, p < 0.001) and after 24 hours (r = 0.80, p < 0.001).
32950932	5	99	dep	cytokines	931:939	arg1	interleukin-1β					963:976	interleukin-1β	963:976	interleukin-1β (IL-1β)	963:984	A cumulative standardized composite measure of inflammation was calculated using the cytokines interleukin-6 (IL-6), interleukin-1β (IL-1β), and interferon-γ (IFN-γ).
32950932	5	99	dep	cytokines	931:939	arg1	interferon-γ					991:1002	interferon-γ	991:1002	interferon-γ (IFN-γ)	991:1010	A cumulative standardized composite measure of inflammation was calculated using the cytokines interleukin-6 (IL-6), interleukin-1β (IL-1β), and interferon-γ (IFN-γ).
32950932	5	99	dep	cytokines	931:939	arg1	cytokines					931:939	the cytokines	927:939	the cytokines interleukin-6 (IL-6), interleukin-1β (IL-1β), and interferon-γ (IFN-γ)	927:1010	A cumulative standardized composite measure of inflammation was calculated using the cytokines interleukin-6 (IL-6), interleukin-1β (IL-1β), and interferon-γ (IFN-γ).
32950932	5	99	dep	cytokines	931:939	arg1	IFN-γ					1005:1009	IFN-γ	1005:1009	IFN-γ	1005:1009	A cumulative standardized composite measure of inflammation was calculated using the cytokines interleukin-6 (IL-6), interleukin-1β (IL-1β), and interferon-γ (IFN-γ).
32950932	5	99	dep	cytokines	931:939	arg1	interleukin-6					941:953	interleukin-6	941:953	interleukin-6 (IL-6)	941:960	A cumulative standardized composite measure of inflammation was calculated using the cytokines interleukin-6 (IL-6), interleukin-1β (IL-1β), and interferon-γ (IFN-γ).
32950932	5	99	dep	cytokines	931:939	arg1	IL-1β					979:983	IL-1β	979:983	IL-1β	979:983	A cumulative standardized composite measure of inflammation was calculated using the cytokines interleukin-6 (IL-6), interleukin-1β (IL-1β), and interferon-γ (IFN-γ).
32950932	5	99	dep	cytokines	931:939	arg1	IL-6					956:959	IL-6	956:959	IL-6	956:959	A cumulative standardized composite measure of inflammation was calculated using the cytokines interleukin-6 (IL-6), interleukin-1β (IL-1β), and interferon-γ (IFN-γ).
32950932	1	100	theme	similar	294:300	arg1	levels					302:307	similar levels	294:307	similar levels of physiological activation	294:335	BACKGROUND Empirical and theoretical evidence suggest that because of the co-evolution of the endocrine and immune response systems, different types of stressors may lead to similar levels of physiological activation.
32950932	9	101	theme	r	1833:1833	arg1	incubation					1821:1830	incubation	1821:1830	incubation (r = 0.80, p < 0.001)	1821:1852	Stimulated inflammatory cytokine production was strongly correlated across the two visits (2 weeks apart) after 4 hours of incubation (r = 0.80, p < 0.001) and after 24 hours (r = 0.80, p < 0.001).
32950932	9	101	theme	r	1833:1833	arg1	0.80					1837:1840	r = 0.80	1833:1840	r = 0.80	1833:1840	Stimulated inflammatory cytokine production was strongly correlated across the two visits (2 weeks apart) after 4 hours of incubation (r = 0.80, p < 0.001) and after 24 hours (r = 0.80, p < 0.001).
32950932	7	102	dep	=	1397:1397	arg1	6.89					1389:1392	6.89	1389:1392	6.89	1389:1392	RESULTS AUCG was significantly associated with stimulated cytokine production at visit 2 after both 4 hours (B = 6.89; p = 0.007) and 24 hours (B = 7.50; p = 0.005) of incubation, controlling for age, sex, and BMI.
32950932	10	103	theme	cytokine	2124:2131	arg1	production					2133:2142	LPS-stimulated inflammatory cytokine production	2096:2142	LPS-stimulated inflammatory cytokine production	2096:2142	Within each visit, stimulated cytokine production after 4 hours was significantly correlated with stimulated inflammation at 24 hours (r = 0.93-0.94, p < 0.05) CONCLUSIONS: These results suggest that LPS-stimulated inflammatory cytokine production and the cortisol response to the TSST contain comparable information about acute human physiological stress responses.
32950932	2	104	theme	present	342:348	arg1	analyses					350:357	The present analyses	338:357	The present analyses	338:357	The present analyses examined associations between two physiological stress responses: the cortisol response to an acute laboratory stressor and ex vivo lipopolysaccharide (LPS) stimulated inflammatory cytokine production.
32950932	1	105	theme	activation	326:335	arg1	levels					302:307	similar levels	294:307	similar levels of physiological activation	294:335	BACKGROUND Empirical and theoretical evidence suggest that because of the co-evolution of the endocrine and immune response systems, different types of stressors may lead to similar levels of physiological activation.
32950932	8	106	dep	hours	1620:1624	arg1	=					1639:1639	=	1639:1639	=	1639:1639	AUCI was also significantly associated with stimulated cytokine production at visit 2 after 4 hours (B = 6.28; p = 0.004) and 24 hours (B = 6.16; p = 0.007) of incubation, controlling for age, sex, and BMI.
32950932	8	107	theme	cytokine	1546:1553	arg1	production					1555:1564	cytokine production	1546:1564	cytokine production	1546:1564	AUCI was also significantly associated with stimulated cytokine production at visit 2 after 4 hours (B = 6.28; p = 0.004) and 24 hours (B = 6.16; p = 0.007) of incubation, controlling for age, sex, and BMI.
32950932	7	108	dep	=	1432:1432	arg1	7.50					1424:1427	7.50	1424:1427	7.50	1424:1427	RESULTS AUCG was significantly associated with stimulated cytokine production at visit 2 after both 4 hours (B = 6.89; p = 0.007) and 24 hours (B = 7.50; p = 0.005) of incubation, controlling for age, sex, and BMI.
32950932	4	109	theme	incubation	825:834	arg1	hours					816:820	4 and 24 hours	807:820	4 and 24 hours of incubation with LPS	807:843	Blood was collected at each appointment to measure circulating inflammatory cytokine levels and stimulated inflammatory cytokine production after 4 and 24 hours of incubation with LPS.
34423963	11	0	theme	biobased	1743:1750	arg1	platforms					1752:1760	biobased platforms	1743:1760	biobased platforms	1743:1760	We envision that such systems will find applications for designing biobased platforms with programmed stability and drug delivery functions.
34423963	7	1	theme	CNCs	1150:1153	arg1	CNCs					1150:1153	CNCs	1150:1153	CNCs	1150:1153	For example, PLA/CNC beads containing 37.5 wt % of CNCs showed a biphasic release of DOX, where 41 and 82% of the loaded drug were released at pH 7.4 and pH 5.5, respectively, over 7 days.
34423963	7	1	theme	CNCs	1150:1153	arg1	%					1145:1145	37.5 wt %	1137:1145	37.5 wt % of CNCs	1137:1153	For example, PLA/CNC beads containing 37.5 wt % of CNCs showed a biphasic release of DOX, where 41 and 82% of the loaded drug were released at pH 7.4 and pH 5.5, respectively, over 7 days.
34423963	0	2	theme	Molecular	83:91	arg1	Encapsulation					93:105	Pharmaceutically Relevant Molecular Encapsulation	57:105	Pharmaceutically Relevant Molecular Encapsulation	57:105	Polymeric Composite Matrix with High Biobased Content as Pharmaceutically Relevant Molecular Encapsulation and Release Platform.
34423963	9	3	theme	PLA/CNC	1461:1467	arg1	beads					1479:1483	drug-loaded PLA/CNC composite beads	1449:1483	drug-loaded PLA/CNC composite beads	1449:1483	We showed that DOX released from drug-loaded PLA/CNC composite beads locally suppressed the growth and proliferation of triple-negative breast cancer cells, MBA-MB-231, via the apoptotic pathway.
34423963	6	4	theme	composite	1007:1015	arg1	beads					1017:1021	composite beads	1007:1021	composite beads	1007:1021	When loaded with DOX, composite beads significantly controlled the release of the drug in a pH-dependent pattern.
34423963	0	5	theme	Release	111:117	arg1	Platform					119:126	Release Platform	111:126	Release Platform	111:126	Polymeric Composite Matrix with High Biobased Content as Pharmaceutically Relevant Molecular Encapsulation and Release Platform.
34423963	3	6	theme	triple-negative	646:660	arg1	cancer					669:674	triple-negative breast cancer	646:674	triple-negative breast cancer	646:674	The performance of the platform was evaluated using doxorubicin (DOX) as a model drug for applications in triple-negative breast cancer.
34423963	11	7	theme	drug	1792:1795	arg1	functions					1806:1814	drug delivery functions	1792:1814	drug delivery functions	1792:1814	We envision that such systems will find applications for designing biobased platforms with programmed stability and drug delivery functions.
34423963	10	8	theme	DDS	1632:1634	arg1	efficacy					1616:1623	The efficacy	1612:1623	The efficacy of the DDS	1612:1634	The efficacy of the DDS was evaluated in human tissue explants.
34423963	6	9	theme	drug	1067:1070	arg1	release					1052:1058	the release	1048:1058	the release of the drug in a pH-dependent pattern	1048:1096	When loaded with DOX, composite beads significantly controlled the release of the drug in a pH-dependent pattern.
34423963	6	10	theme	pH-dependent	1077:1088	arg1	pattern					1090:1096	a pH-dependent pattern	1075:1096	a pH-dependent pattern	1075:1096	When loaded with DOX, composite beads significantly controlled the release of the drug in a pH-dependent pattern.
34423963	9	11	theme	triple-negative	1536:1550	arg1	MBA-MB-231					1573:1582	MBA-MB-231	1573:1582	MBA-MB-231	1573:1582	We showed that DOX released from drug-loaded PLA/CNC composite beads locally suppressed the growth and proliferation of triple-negative breast cancer cells, MBA-MB-231, via the apoptotic pathway.
34423963	9	11	theme	triple-negative	1536:1550	arg1	cells					1566:1570	triple-negative breast cancer cells	1536:1570	triple-negative breast cancer cells	1536:1570	We showed that DOX released from drug-loaded PLA/CNC composite beads locally suppressed the growth and proliferation of triple-negative breast cancer cells, MBA-MB-231, via the apoptotic pathway.
34423963	1	12	theme	many	273:276	arg1	settings					287:294	many clinical settings	273:294	many clinical settings	273:294	Drug delivery systems (DDS) that can temporally control the rate and extent of release of therapeutically active molecules find applications in many clinical settings, ranging from infection control to cancer therapy.
34423963	2	13	theme	l-lactic	502:509	arg1	poly					496:499	cellulose nanocrystal (CNC)-reinforced poly	457:499	cellulose nanocrystal (CNC)-reinforced poly (l-lactic acid) (PLA) composite beads	457:537	With an aim to design a locally implantable, controlled-release DDS, we demonstrated the feasibility of using cellulose nanocrystal (CNC)-reinforced poly (l-lactic acid) (PLA) composite beads.
34423963	2	13	theme	l-lactic	502:509	arg1	acid					511:514	l-lactic acid	502:514	l-lactic acid	502:514	With an aim to design a locally implantable, controlled-release DDS, we demonstrated the feasibility of using cellulose nanocrystal (CNC)-reinforced poly (l-lactic acid) (PLA) composite beads.
34423963	5	14	theme	mechanical	852:861	arg1	activity					938:945	pharmacological activity	922:945	pharmacological activity	922:945	We observed that CNC loading within these beads played a critical role in the mechanical stability, porosity, water uptake, diffusion, release, and pharmacological activity of the drug from the delivery system.
34423963	5	14	theme	mechanical	852:861	arg1	stability					863:871	the mechanical stability	848:871	the mechanical stability	848:871	We observed that CNC loading within these beads played a critical role in the mechanical stability, porosity, water uptake, diffusion, release, and pharmacological activity of the drug from the delivery system.
34423963	5	14	theme	mechanical	852:861	arg1	diffusion					898:906	diffusion	898:906	diffusion	898:906	We observed that CNC loading within these beads played a critical role in the mechanical stability, porosity, water uptake, diffusion, release, and pharmacological activity of the drug from the delivery system.
34423963	5	14	theme	mechanical	852:861	arg1	porosity					874:881	porosity	874:881	porosity	874:881	We observed that CNC loading within these beads played a critical role in the mechanical stability, porosity, water uptake, diffusion, release, and pharmacological activity of the drug from the delivery system.
34423963	5	14	theme	mechanical	852:861	arg1	release					909:915	release	909:915	release	909:915	We observed that CNC loading within these beads played a critical role in the mechanical stability, porosity, water uptake, diffusion, release, and pharmacological activity of the drug from the delivery system.
34423963	5	14	theme	mechanical	852:861	arg1	uptake					890:895	water uptake	884:895	water uptake	884:895	We observed that CNC loading within these beads played a critical role in the mechanical stability, porosity, water uptake, diffusion, release, and pharmacological activity of the drug from the delivery system.
34423963	9	15	theme	breast	1552:1557	arg1	MBA-MB-231					1573:1582	MBA-MB-231	1573:1582	MBA-MB-231	1573:1582	We showed that DOX released from drug-loaded PLA/CNC composite beads locally suppressed the growth and proliferation of triple-negative breast cancer cells, MBA-MB-231, via the apoptotic pathway.
34423963	9	15	theme	breast	1552:1557	arg1	cells					1566:1570	triple-negative breast cancer cells	1536:1570	triple-negative breast cancer cells	1536:1570	We showed that DOX released from drug-loaded PLA/CNC composite beads locally suppressed the growth and proliferation of triple-negative breast cancer cells, MBA-MB-231, via the apoptotic pathway.
34423963	7	16	theme	PLA/CNC	1112:1118	arg1	beads					1120:1124	PLA/CNC beads	1112:1124	PLA/CNC beads containing 37.5 wt % of CNCs	1112:1153	For example, PLA/CNC beads containing 37.5 wt % of CNCs showed a biphasic release of DOX, where 41 and 82% of the loaded drug were released at pH 7.4 and pH 5.5, respectively, over 7 days.
34423963	9	17	theme	cancer	1559:1564	arg1	MBA-MB-231					1573:1582	MBA-MB-231	1573:1582	MBA-MB-231	1573:1582	We showed that DOX released from drug-loaded PLA/CNC composite beads locally suppressed the growth and proliferation of triple-negative breast cancer cells, MBA-MB-231, via the apoptotic pathway.
34423963	9	17	theme	cancer	1559:1564	arg1	cells					1566:1570	triple-negative breast cancer cells	1536:1570	triple-negative breast cancer cells	1536:1570	We showed that DOX released from drug-loaded PLA/CNC composite beads locally suppressed the growth and proliferation of triple-negative breast cancer cells, MBA-MB-231, via the apoptotic pathway.
34423963	2	18	theme	implantable	379:389	arg1	DDS					411:413	a locally implantable, controlled-release DDS	369:413	a locally implantable, controlled-release DDS	369:413	With an aim to design a locally implantable, controlled-release DDS, we demonstrated the feasibility of using cellulose nanocrystal (CNC)-reinforced poly (l-lactic acid) (PLA) composite beads.
34423963	0	19	theme	Composite	10:18	arg1	Matrix					20:25	Polymeric Composite Matrix	0:25	Polymeric Composite Matrix with High Biobased Content as Pharmaceutically Relevant Molecular Encapsulation and Release Platform.	0:127	Polymeric Composite Matrix with High Biobased Content as Pharmaceutically Relevant Molecular Encapsulation and Release Platform.
34423963	3	20	from	applications	630:641	arg1	cancer					669:674	triple-negative breast cancer	646:674	triple-negative breast cancer	646:674	The performance of the platform was evaluated using doxorubicin (DOX) as a model drug for applications in triple-negative breast cancer.
34423963	7	21	theme	wt	1142:1143	arg1	CNCs					1150:1153	CNCs	1150:1153	CNCs	1150:1153	For example, PLA/CNC beads containing 37.5 wt % of CNCs showed a biphasic release of DOX, where 41 and 82% of the loaded drug were released at pH 7.4 and pH 5.5, respectively, over 7 days.
34423963	7	21	theme	wt	1142:1143	arg1	%					1145:1145	37.5 wt %	1137:1145	37.5 wt % of CNCs	1137:1153	For example, PLA/CNC beads containing 37.5 wt % of CNCs showed a biphasic release of DOX, where 41 and 82% of the loaded drug were released at pH 7.4 and pH 5.5, respectively, over 7 days.
34423963	0	22	theme	Relevant	74:81	arg1	Encapsulation					93:105	Pharmaceutically Relevant Molecular Encapsulation	57:105	Pharmaceutically Relevant Molecular Encapsulation	57:105	Polymeric Composite Matrix with High Biobased Content as Pharmaceutically Relevant Molecular Encapsulation and Release Platform.
34423963	4	23	theme	phase	706:710	arg1	separation					712:721	phase separation	706:721	A facile, nonsolvent-induced phase separation (NIPS) method	677:735	A facile, nonsolvent-induced phase separation (NIPS) method was adopted to form composite beads.
34423963	4	23	theme	phase	706:710	arg1	NIPS					724:727	NIPS	724:727	NIPS	724:727	A facile, nonsolvent-induced phase separation (NIPS) method was adopted to form composite beads.
34423963	9	24	theme	cells	1566:1570	arg1	proliferation					1519:1531	proliferation	1519:1531	proliferation	1519:1531	We showed that DOX released from drug-loaded PLA/CNC composite beads locally suppressed the growth and proliferation of triple-negative breast cancer cells, MBA-MB-231, via the apoptotic pathway.
34423963	9	24	theme	cells	1566:1570	arg1	growth					1508:1513	growth	1508:1513	growth	1508:1513	We showed that DOX released from drug-loaded PLA/CNC composite beads locally suppressed the growth and proliferation of triple-negative breast cancer cells, MBA-MB-231, via the apoptotic pathway.
34423963	11	25	theme	programmed	1767:1776	arg1	stability					1778:1786	programmed stability	1767:1786	programmed stability	1767:1786	We envision that such systems will find applications for designing biobased platforms with programmed stability and drug delivery functions.
34423963	0	26	theme	Polymeric	0:8	arg1	Matrix					20:25	Polymeric Composite Matrix	0:25	Polymeric Composite Matrix with High Biobased Content as Pharmaceutically Relevant Molecular Encapsulation and Release Platform.	0:127	Polymeric Composite Matrix with High Biobased Content as Pharmaceutically Relevant Molecular Encapsulation and Release Platform.
34423963	1	27	theme	clinical	278:285	arg1	settings					287:294	many clinical settings	273:294	many clinical settings	273:294	Drug delivery systems (DDS) that can temporally control the rate and extent of release of therapeutically active molecules find applications in many clinical settings, ranging from infection control to cancer therapy.
34423963	4	28	dep	facile	679:684	arg1	nonsolvent-induced					687:704	nonsolvent-induced	687:704	nonsolvent-induced	687:704	A facile, nonsolvent-induced phase separation (NIPS) method was adopted to form composite beads.
34423963	7	29	theme	drug	1220:1223	arg1	drug					1220:1223	the loaded drug	1209:1223	the loaded drug	1209:1223	For example, PLA/CNC beads containing 37.5 wt % of CNCs showed a biphasic release of DOX, where 41 and 82% of the loaded drug were released at pH 7.4 and pH 5.5, respectively, over 7 days.
34423963	7	29	theme	drug	1220:1223	arg1	41					1195:1196	41	1195:1196	41	1195:1196	For example, PLA/CNC beads containing 37.5 wt % of CNCs showed a biphasic release of DOX, where 41 and 82% of the loaded drug were released at pH 7.4 and pH 5.5, respectively, over 7 days.
34423963	2	30	theme	composite	523:531	arg1	beads					533:537	cellulose nanocrystal (CNC)-reinforced poly (l-lactic acid) (PLA) composite beads	457:537	cellulose nanocrystal (CNC)-reinforced poly (l-lactic acid) (PLA) composite beads	457:537	With an aim to design a locally implantable, controlled-release DDS, we demonstrated the feasibility of using cellulose nanocrystal (CNC)-reinforced poly (l-lactic acid) (PLA) composite beads.
34423963	5	31	from	system	977:982	arg1	uptake					890:895	water uptake	884:895	water uptake	884:895	We observed that CNC loading within these beads played a critical role in the mechanical stability, porosity, water uptake, diffusion, release, and pharmacological activity of the drug from the delivery system.
34423963	5	31	from	system	977:982	arg1	activity					938:945	pharmacological activity	922:945	pharmacological activity	922:945	We observed that CNC loading within these beads played a critical role in the mechanical stability, porosity, water uptake, diffusion, release, and pharmacological activity of the drug from the delivery system.
34423963	5	31	from	system	977:982	arg1	release					909:915	release	909:915	release	909:915	We observed that CNC loading within these beads played a critical role in the mechanical stability, porosity, water uptake, diffusion, release, and pharmacological activity of the drug from the delivery system.
34423963	5	31	from	system	977:982	arg1	diffusion					898:906	diffusion	898:906	diffusion	898:906	We observed that CNC loading within these beads played a critical role in the mechanical stability, porosity, water uptake, diffusion, release, and pharmacological activity of the drug from the delivery system.
34423963	5	31	from	system	977:982	arg1	stability					863:871	the mechanical stability	848:871	the mechanical stability	848:871	We observed that CNC loading within these beads played a critical role in the mechanical stability, porosity, water uptake, diffusion, release, and pharmacological activity of the drug from the delivery system.
34423963	9	32	theme	drug-loaded	1449:1459	arg1	beads					1479:1483	drug-loaded PLA/CNC composite beads	1449:1483	drug-loaded PLA/CNC composite beads	1449:1483	We showed that DOX released from drug-loaded PLA/CNC composite beads locally suppressed the growth and proliferation of triple-negative breast cancer cells, MBA-MB-231, via the apoptotic pathway.
34423963	5	33	theme	water	884:888	arg1	uptake					890:895	water uptake	884:895	water uptake	884:895	We observed that CNC loading within these beads played a critical role in the mechanical stability, porosity, water uptake, diffusion, release, and pharmacological activity of the drug from the delivery system.
34423963	5	33	theme	water	884:888	arg1	stability					863:871	the mechanical stability	848:871	the mechanical stability	848:871	We observed that CNC loading within these beads played a critical role in the mechanical stability, porosity, water uptake, diffusion, release, and pharmacological activity of the drug from the delivery system.
34423963	9	34	attach	released	1435:1442	arg2	DOX					1431:1433	DOX	1431:1433	DOX released from drug-loaded PLA/CNC composite beads	1431:1483	We showed that DOX released from drug-loaded PLA/CNC composite beads locally suppressed the growth and proliferation of triple-negative breast cancer cells, MBA-MB-231, via the apoptotic pathway.
34423963	9	34	attach	released	1435:1442	arg1	beads					1479:1483	drug-loaded PLA/CNC composite beads	1449:1483	drug-loaded PLA/CNC composite beads	1449:1483	We showed that DOX released from drug-loaded PLA/CNC composite beads locally suppressed the growth and proliferation of triple-negative breast cancer cells, MBA-MB-231, via the apoptotic pathway.
34423963	4	35	theme	separation	712:721	arg1	method					730:735	A facile, nonsolvent-induced phase separation (NIPS) method	677:735	A facile, nonsolvent-induced phase separation (NIPS) method	677:735	A facile, nonsolvent-induced phase separation (NIPS) method was adopted to form composite beads.
34423963	5	36	theme	CNC	791:793	arg1	loading					795:801	CNC loading	791:801	CNC loading within these beads	791:820	We observed that CNC loading within these beads played a critical role in the mechanical stability, porosity, water uptake, diffusion, release, and pharmacological activity of the drug from the delivery system.
34423963	0	37	theme	Biobased	37:44	arg1	Content					46:52	High Biobased Content	32:52	High Biobased Content as Pharmaceutically Relevant Molecular Encapsulation and Release Platform	32:126	Polymeric Composite Matrix with High Biobased Content as Pharmaceutically Relevant Molecular Encapsulation and Release Platform.
34423963	8	38	theme	Drug	1288:1291	arg1	release					1293:1299	Drug release	1288:1299	Drug release	1288:1299	Drug release followed Korsmeyer's kinetics, indicating that the release mechanism was mostly diffusion and swelling-controlled.
34423963	3	39	theme	platform	563:570	arg1	performance					544:554	The performance	540:554	The performance of the platform	540:570	The performance of the platform was evaluated using doxorubicin (DOX) as a model drug for applications in triple-negative breast cancer.
34423963	11	40	theme	delivery	1797:1804	arg1	functions					1806:1814	drug delivery functions	1792:1814	drug delivery functions	1792:1814	We envision that such systems will find applications for designing biobased platforms with programmed stability and drug delivery functions.
34423963	0	41	theme	High	32:35	arg1	Content					46:52	High Biobased Content	32:52	High Biobased Content as Pharmaceutically Relevant Molecular Encapsulation and Release Platform	32:126	Polymeric Composite Matrix with High Biobased Content as Pharmaceutically Relevant Molecular Encapsulation and Release Platform.
34423963	11	42	theme	such	1693:1696	arg1	systems					1698:1704	such systems	1693:1704	such systems	1693:1704	We envision that such systems will find applications for designing biobased platforms with programmed stability and drug delivery functions.
34423963	1	43	from	applications	257:268	arg1	settings					287:294	many clinical settings	273:294	many clinical settings	273:294	Drug delivery systems (DDS) that can temporally control the rate and extent of release of therapeutically active molecules find applications in many clinical settings, ranging from infection control to cancer therapy.
34423963	1	44	dep	rate	189:192	arg1	the					185:187	the	185:187	the	185:187	Drug delivery systems (DDS) that can temporally control the rate and extent of release of therapeutically active molecules find applications in many clinical settings, ranging from infection control to cancer therapy.
34423963	6	45	from	release	1052:1058	arg1	pattern					1090:1096	a pH-dependent pattern	1075:1096	a pH-dependent pattern	1075:1096	When loaded with DOX, composite beads significantly controlled the release of the drug in a pH-dependent pattern.
34423963	1	46	theme	release	208:214	arg1	extent					198:203	extent	198:203	extent	198:203	Drug delivery systems (DDS) that can temporally control the rate and extent of release of therapeutically active molecules find applications in many clinical settings, ranging from infection control to cancer therapy.
34423963	1	46	theme	release	208:214	arg1	rate					189:192	rate	189:192	rate	189:192	Drug delivery systems (DDS) that can temporally control the rate and extent of release of therapeutically active molecules find applications in many clinical settings, ranging from infection control to cancer therapy.
34423963	1	47	theme	infection	310:318	arg1	control					320:326	infection control	310:326	infection control	310:326	Drug delivery systems (DDS) that can temporally control the rate and extent of release of therapeutically active molecules find applications in many clinical settings, ranging from infection control to cancer therapy.
34423963	9	48	theme	apoptotic	1593:1601	arg1	pathway					1603:1609	the apoptotic pathway	1589:1609	the apoptotic pathway	1589:1609	We showed that DOX released from drug-loaded PLA/CNC composite beads locally suppressed the growth and proliferation of triple-negative breast cancer cells, MBA-MB-231, via the apoptotic pathway.
34423963	7	49	theme	DOX	1184:1186	arg1	release					1173:1179	a biphasic release	1162:1179	a biphasic release	1162:1179	For example, PLA/CNC beads containing 37.5 wt % of CNCs showed a biphasic release of DOX, where 41 and 82% of the loaded drug were released at pH 7.4 and pH 5.5, respectively, over 7 days.
34423963	9	50	theme	composite	1469:1477	arg1	beads					1479:1483	drug-loaded PLA/CNC composite beads	1449:1483	drug-loaded PLA/CNC composite beads	1449:1483	We showed that DOX released from drug-loaded PLA/CNC composite beads locally suppressed the growth and proliferation of triple-negative breast cancer cells, MBA-MB-231, via the apoptotic pathway.
34423963	0	51	with	Matrix	20:25	arg1	Content					46:52	High Biobased Content	32:52	High Biobased Content as Pharmaceutically Relevant Molecular Encapsulation and Release Platform	32:126	Polymeric Composite Matrix with High Biobased Content as Pharmaceutically Relevant Molecular Encapsulation and Release Platform.
34423963	1	52	theme	Drug	129:132	arg1	systems					143:149	Drug delivery systems	129:149	Drug delivery systems (DDS) that can temporally control the rate and extent of release of therapeutically active molecules	129:250	Drug delivery systems (DDS) that can temporally control the rate and extent of release of therapeutically active molecules find applications in many clinical settings, ranging from infection control to cancer therapy.
34423963	1	52	theme	Drug	129:132	arg1	DDS					152:154	DDS	152:154	DDS	152:154	Drug delivery systems (DDS) that can temporally control the rate and extent of release of therapeutically active molecules find applications in many clinical settings, ranging from infection control to cancer therapy.
34423963	8	53	theme	release	1352:1358	arg1	mechanism					1360:1368	the release mechanism	1348:1368	the release mechanism	1348:1368	Drug release followed Korsmeyer's kinetics, indicating that the release mechanism was mostly diffusion and swelling-controlled.
34423963	8	53	theme	release	1352:1358	arg1	diffusion					1381:1389	diffusion	1381:1389	diffusion	1381:1389	Drug release followed Korsmeyer's kinetics, indicating that the release mechanism was mostly diffusion and swelling-controlled.
34423963	3	54	theme	breast	662:667	arg1	cancer					669:674	triple-negative breast cancer	646:674	triple-negative breast cancer	646:674	The performance of the platform was evaluated using doxorubicin (DOX) as a model drug for applications in triple-negative breast cancer.
34423963	5	55	theme	delivery	968:975	arg1	system					977:982	the delivery system	964:982	the delivery system	964:982	We observed that CNC loading within these beads played a critical role in the mechanical stability, porosity, water uptake, diffusion, release, and pharmacological activity of the drug from the delivery system.
34423963	10	56	theme	tissue	1659:1664	arg1	explants					1666:1673	human tissue explants	1653:1673	human tissue explants	1653:1673	The efficacy of the DDS was evaluated in human tissue explants.
34423963	7	57	contain	containing	1126:1135	arg1	beads					1120:1124	PLA/CNC beads	1112:1124	PLA/CNC beads containing 37.5 wt % of CNCs	1112:1153	For example, PLA/CNC beads containing 37.5 wt % of CNCs showed a biphasic release of DOX, where 41 and 82% of the loaded drug were released at pH 7.4 and pH 5.5, respectively, over 7 days.
34423963	7	57	contain	containing	1126:1135	arg2	CNCs					1150:1153	CNCs	1150:1153	CNCs	1150:1153	For example, PLA/CNC beads containing 37.5 wt % of CNCs showed a biphasic release of DOX, where 41 and 82% of the loaded drug were released at pH 7.4 and pH 5.5, respectively, over 7 days.
34423963	7	57	contain	containing	1126:1135	arg2	%					1145:1145	37.5 wt %	1137:1145	37.5 wt % of CNCs	1137:1153	For example, PLA/CNC beads containing 37.5 wt % of CNCs showed a biphasic release of DOX, where 41 and 82% of the loaded drug were released at pH 7.4 and pH 5.5, respectively, over 7 days.
34423963	1	58	theme	delivery	134:141	arg1	systems					143:149	Drug delivery systems	129:149	Drug delivery systems (DDS) that can temporally control the rate and extent of release of therapeutically active molecules	129:250	Drug delivery systems (DDS) that can temporally control the rate and extent of release of therapeutically active molecules find applications in many clinical settings, ranging from infection control to cancer therapy.
34423963	1	58	theme	delivery	134:141	arg1	DDS					152:154	DDS	152:154	DDS	152:154	Drug delivery systems (DDS) that can temporally control the rate and extent of release of therapeutically active molecules find applications in many clinical settings, ranging from infection control to cancer therapy.
34423963	5	59	theme	drug	954:957	arg1	uptake					890:895	water uptake	884:895	water uptake	884:895	We observed that CNC loading within these beads played a critical role in the mechanical stability, porosity, water uptake, diffusion, release, and pharmacological activity of the drug from the delivery system.
34423963	5	59	theme	drug	954:957	arg1	activity					938:945	pharmacological activity	922:945	pharmacological activity	922:945	We observed that CNC loading within these beads played a critical role in the mechanical stability, porosity, water uptake, diffusion, release, and pharmacological activity of the drug from the delivery system.
34423963	5	59	theme	drug	954:957	arg1	release					909:915	release	909:915	release	909:915	We observed that CNC loading within these beads played a critical role in the mechanical stability, porosity, water uptake, diffusion, release, and pharmacological activity of the drug from the delivery system.
34423963	5	59	theme	drug	954:957	arg1	diffusion					898:906	diffusion	898:906	diffusion	898:906	We observed that CNC loading within these beads played a critical role in the mechanical stability, porosity, water uptake, diffusion, release, and pharmacological activity of the drug from the delivery system.
34423963	5	59	theme	drug	954:957	arg1	stability					863:871	the mechanical stability	848:871	the mechanical stability	848:871	We observed that CNC loading within these beads played a critical role in the mechanical stability, porosity, water uptake, diffusion, release, and pharmacological activity of the drug from the delivery system.
34423963	3	60	theme	model	615:619	arg1	drug					621:624	a model drug	613:624	a model drug for applications in triple-negative breast cancer	613:674	The performance of the platform was evaluated using doxorubicin (DOX) as a model drug for applications in triple-negative breast cancer.
34423963	3	60	theme	model	615:619	arg1	doxorubicin					592:602	doxorubicin	592:602	doxorubicin (DOX)	592:608	The performance of the platform was evaluated using doxorubicin (DOX) as a model drug for applications in triple-negative breast cancer.
34423963	5	61	theme	pharmacological	922:936	arg1	activity					938:945	pharmacological activity	922:945	pharmacological activity	922:945	We observed that CNC loading within these beads played a critical role in the mechanical stability, porosity, water uptake, diffusion, release, and pharmacological activity of the drug from the delivery system.
34423963	5	61	theme	pharmacological	922:936	arg1	stability					863:871	the mechanical stability	848:871	the mechanical stability	848:871	We observed that CNC loading within these beads played a critical role in the mechanical stability, porosity, water uptake, diffusion, release, and pharmacological activity of the drug from the delivery system.
34423963	4	62	theme	facile	679:684	arg1	method					730:735	A facile, nonsolvent-induced phase separation (NIPS) method	677:735	A facile, nonsolvent-induced phase separation (NIPS) method	677:735	A facile, nonsolvent-induced phase separation (NIPS) method was adopted to form composite beads.
34423963	10	63	theme	human	1653:1657	arg1	explants					1666:1673	human tissue explants	1653:1673	human tissue explants	1653:1673	The efficacy of the DDS was evaluated in human tissue explants.
34423963	2	64	theme	poly	496:499	arg1	beads					533:537	cellulose nanocrystal (CNC)-reinforced poly (l-lactic acid) (PLA) composite beads	457:537	cellulose nanocrystal (CNC)-reinforced poly (l-lactic acid) (PLA) composite beads	457:537	With an aim to design a locally implantable, controlled-release DDS, we demonstrated the feasibility of using cellulose nanocrystal (CNC)-reinforced poly (l-lactic acid) (PLA) composite beads.
34423963	4	65	theme	composite	757:765	arg1	beads					767:771	composite beads	757:771	composite beads	757:771	A facile, nonsolvent-induced phase separation (NIPS) method was adopted to form composite beads.
34423963	5	66	theme	critical	831:838	arg1	role					840:843	a critical role	829:843	a critical role	829:843	We observed that CNC loading within these beads played a critical role in the mechanical stability, porosity, water uptake, diffusion, release, and pharmacological activity of the drug from the delivery system.
34423963	7	67	theme	loaded	1213:1218	arg1	drug					1220:1223	the loaded drug	1209:1223	the loaded drug	1209:1223	For example, PLA/CNC beads containing 37.5 wt % of CNCs showed a biphasic release of DOX, where 41 and 82% of the loaded drug were released at pH 7.4 and pH 5.5, respectively, over 7 days.
34423963	1	68	theme	active	235:240	arg1	molecules					242:250	therapeutically active molecules	219:250	therapeutically active molecules	219:250	Drug delivery systems (DDS) that can temporally control the rate and extent of release of therapeutically active molecules find applications in many clinical settings, ranging from infection control to cancer therapy.
34423963	2	69	theme	-reinforced	484:494	arg1	poly					496:499	cellulose nanocrystal (CNC)-reinforced poly	457:499	cellulose nanocrystal (CNC)-reinforced poly (l-lactic acid) (PLA) composite beads	457:537	With an aim to design a locally implantable, controlled-release DDS, we demonstrated the feasibility of using cellulose nanocrystal (CNC)-reinforced poly (l-lactic acid) (PLA) composite beads.
34423963	2	69	theme	-reinforced	484:494	arg1	acid					511:514	l-lactic acid	502:514	l-lactic acid	502:514	With an aim to design a locally implantable, controlled-release DDS, we demonstrated the feasibility of using cellulose nanocrystal (CNC)-reinforced poly (l-lactic acid) (PLA) composite beads.
34423963	2	69	theme	-reinforced	484:494	arg1	PLA					518:520	PLA	518:520	PLA	518:520	With an aim to design a locally implantable, controlled-release DDS, we demonstrated the feasibility of using cellulose nanocrystal (CNC)-reinforced poly (l-lactic acid) (PLA) composite beads.
34423963	1	70	theme	cancer	331:336	arg1	therapy					338:344	cancer therapy	331:344	cancer therapy	331:344	Drug delivery systems (DDS) that can temporally control the rate and extent of release of therapeutically active molecules find applications in many clinical settings, ranging from infection control to cancer therapy.
34423963	2	71	dep	implantable	379:389	arg1	controlled-release					392:409	controlled-release	392:409	controlled-release	392:409	With an aim to design a locally implantable, controlled-release DDS, we demonstrated the feasibility of using cellulose nanocrystal (CNC)-reinforced poly (l-lactic acid) (PLA) composite beads.
34423963	7	72	theme	biphasic	1164:1171	arg1	release					1173:1179	a biphasic release	1162:1179	a biphasic release	1162:1179	For example, PLA/CNC beads containing 37.5 wt % of CNCs showed a biphasic release of DOX, where 41 and 82% of the loaded drug were released at pH 7.4 and pH 5.5, respectively, over 7 days.
34423963	1	73	theme	molecules	242:250	arg1	release					208:214	release	208:214	release of therapeutically active molecules	208:250	Drug delivery systems (DDS) that can temporally control the rate and extent of release of therapeutically active molecules find applications in many clinical settings, ranging from infection control to cancer therapy.
33771785	5	0	theme	microbial	806:814	arg1	distinct					850:857	distinct	850:857	distinct	850:857	The bacterial and fungal microbial communities in amended soils were distinct from the microbial communities in nonamended soil, as well as those in soils that received other nonsuppressive treatments.
33771785	5	0	theme	microbial	806:814	arg1	communities					816:826	The bacterial and fungal microbial communities	781:826	The bacterial and fungal microbial communities in amended soils	781:843	The bacterial and fungal microbial communities in amended soils were distinct from the microbial communities in nonamended soil, as well as those in soils that received other nonsuppressive treatments.
33771785	7	1	theme	microbial	1186:1194	arg1	community					1196:1204	The microbial community	1182:1204	The microbial community in keratin- and chitin-amended soils	1182:1241	The microbial community in keratin- and chitin-amended soils was associated with higher zinc, copper, and selenium, respectively.IMPORTANCE Our results highlight the importance of soil microorganisms in plant disease suppression and the possibility to steer soil microbial community composition by applying organic amendments to the soil.
33771785	2	2	theme	fungal	364:369	arg1	pathogens					371:379	fungal pathogens	364:379	fungal pathogens	364:379	Organic amendments have been shown to reduce crop diseases and pests, with chitin products the most efficient against fungal pathogens.
33771785	5	3	theme	bacterial	785:793	arg1	distinct					850:857	distinct	850:857	distinct	850:857	The bacterial and fungal microbial communities in amended soils were distinct from the microbial communities in nonamended soil, as well as those in soils that received other nonsuppressive treatments.
33771785	5	3	theme	bacterial	785:793	arg1	communities					816:826	The bacterial and fungal microbial communities	781:826	The bacterial and fungal microbial communities in amended soils	781:843	The bacterial and fungal microbial communities in amended soils were distinct from the microbial communities in nonamended soil, as well as those in soils that received other nonsuppressive treatments.
33771785	6	4	theme	chitinolytic	1112:1123	arg1	properties					1125:1134	their keratinolytic and chitinolytic properties	1088:1134	their keratinolytic and chitinolytic properties	1088:1134	The Rhizoctonia-suppressive amended soils were rich in saprophytic bacteria and fungi that are known for their keratinolytic and chitinolytic properties (i.e., Oxalobacteraceae and Mortierellaceae).
33771785	4	5	theme	solani	735:740	arg1	symptoms					750:757	Rhizoctonia solani disease symptoms	723:757	Rhizoctonia solani disease symptoms	723:757	Organic amendments rich in keratin or chitin reduced Rhizoctonia solani disease symptoms in sugar beet plants.
33771785	6	6	theme	saprophytic	1038:1048	arg1	bacteria					1050:1057	bacteria	1050:1057	bacteria	1050:1057	The Rhizoctonia-suppressive amended soils were rich in saprophytic bacteria and fungi that are known for their keratinolytic and chitinolytic properties (i.e., Oxalobacteraceae and Mortierellaceae).
33771785	7	7	theme	microorganisms	1367:1380	arg1	importance					1348:1357	the importance	1344:1357	the importance of soil microorganisms in plant disease suppression	1344:1409	The microbial community in keratin- and chitin-amended soils was associated with higher zinc, copper, and selenium, respectively.IMPORTANCE Our results highlight the importance of soil microorganisms in plant disease suppression and the possibility to steer soil microbial community composition by applying organic amendments to the soil.
33771785	0	8	theme	Soil	96:99	arg1	Community					111:119	the Soil Microbial Community	92:119	the Soil Microbial Community	92:119	Chitin- and Keratin-Rich Soil Amendments Suppress Rhizoctonia solani Disease via Changes to the Soil Microbial Community.
33771785	7	9	theme	microbial	1445:1453	arg1	composition					1465:1475	soil microbial community composition	1440:1475	soil microbial community composition	1440:1475	The microbial community in keratin- and chitin-amended soils was associated with higher zinc, copper, and selenium, respectively.IMPORTANCE Our results highlight the importance of soil microorganisms in plant disease suppression and the possibility to steer soil microbial community composition by applying organic amendments to the soil.
33771785	7	10	theme	community	1455:1463	arg1	composition					1465:1475	soil microbial community composition	1440:1475	soil microbial community composition	1440:1475	The microbial community in keratin- and chitin-amended soils was associated with higher zinc, copper, and selenium, respectively.IMPORTANCE Our results highlight the importance of soil microorganisms in plant disease suppression and the possibility to steer soil microbial community composition by applying organic amendments to the soil.
33771785	3	11	theme	physicochemical	556:570	arg1	properties					572:581	different physicochemical properties	546:581	different physicochemical properties	546:581	To study which characteristics of organic products are correlated with disease suppression, an experiment was designed in which 10 types of organic amendments with different physicochemical properties were tested against the soilborne pathogen Rhizoctonia solani in sugar beet seedlings.
33771785	7	12	dep	respectively.IMPORTANCE	1298:1320	arg1	highlight					1334:1342	highlight	1334:1342	highlight the importance of soil microorganisms in plant disease suppression	1334:1409	The microbial community in keratin- and chitin-amended soils was associated with higher zinc, copper, and selenium, respectively.IMPORTANCE Our results highlight the importance of soil microorganisms in plant disease suppression and the possibility to steer soil microbial community composition by applying organic amendments to the soil.
33771785	3	13	with	amendments	530:539	arg1	properties					572:581	different physicochemical properties	546:581	different physicochemical properties	546:581	To study which characteristics of organic products are correlated with disease suppression, an experiment was designed in which 10 types of organic amendments with different physicochemical properties were tested against the soilborne pathogen Rhizoctonia solani in sugar beet seedlings.
33771785	4	14	theme	disease	742:748	arg1	symptoms					750:757	Rhizoctonia solani disease symptoms	723:757	Rhizoctonia solani disease symptoms	723:757	Organic amendments rich in keratin or chitin reduced Rhizoctonia solani disease symptoms in sugar beet plants.
33771785	4	15	from	chitin	708:713	arg1	rich					689:692	rich	689:692	rich	689:692	Organic amendments rich in keratin or chitin reduced Rhizoctonia solani disease symptoms in sugar beet plants.
33771785	4	16	theme	sugar	762:766	arg1	plants					773:778	sugar beet plants	762:778	sugar beet plants	762:778	Organic amendments rich in keratin or chitin reduced Rhizoctonia solani disease symptoms in sugar beet plants.
33771785	7	17	theme	higher	1263:1268	arg1	zinc					1270:1273	higher zinc	1263:1273	higher zinc	1263:1273	The microbial community in keratin- and chitin-amended soils was associated with higher zinc, copper, and selenium, respectively.IMPORTANCE Our results highlight the importance of soil microorganisms in plant disease suppression and the possibility to steer soil microbial community composition by applying organic amendments to the soil.
33771785	6	18	from	bacteria	1050:1057	arg1	rich					1030:1033	rich	1030:1033	rich	1030:1033	The Rhizoctonia-suppressive amended soils were rich in saprophytic bacteria and fungi that are known for their keratinolytic and chitinolytic properties (i.e., Oxalobacteraceae and Mortierellaceae).
33771785	6	18	from	bacteria	1050:1057	arg1	soils					1019:1023	The Rhizoctonia-suppressive amended soils	983:1023	The Rhizoctonia-suppressive amended soils	983:1023	The Rhizoctonia-suppressive amended soils were rich in saprophytic bacteria and fungi that are known for their keratinolytic and chitinolytic properties (i.e., Oxalobacteraceae and Mortierellaceae).
33771785	7	19	theme	disease	1391:1397	arg1	suppression					1399:1409	plant disease suppression	1385:1409	plant disease suppression	1385:1409	The microbial community in keratin- and chitin-amended soils was associated with higher zinc, copper, and selenium, respectively.IMPORTANCE Our results highlight the importance of soil microorganisms in plant disease suppression and the possibility to steer soil microbial community composition by applying organic amendments to the soil.
33771785	2	20	theme	crop	291:294	arg1	diseases					296:303	crop diseases	291:303	crop diseases	291:303	Organic amendments have been shown to reduce crop diseases and pests, with chitin products the most efficient against fungal pathogens.
33771785	5	21	theme	nonsuppressive	956:969	arg1	treatments					971:980	other nonsuppressive treatments	950:980	other nonsuppressive treatments	950:980	The bacterial and fungal microbial communities in amended soils were distinct from the microbial communities in nonamended soil, as well as those in soils that received other nonsuppressive treatments.
33771785	3	22	theme	disease	453:459	arg1	suppression					461:471	disease suppression	453:471	disease suppression	453:471	To study which characteristics of organic products are correlated with disease suppression, an experiment was designed in which 10 types of organic amendments with different physicochemical properties were tested against the soilborne pathogen Rhizoctonia solani in sugar beet seedlings.
33771785	0	23	theme	Microbial	101:109	arg1	Community					111:119	the Soil Microbial Community	92:119	the Soil Microbial Community	92:119	Chitin- and Keratin-Rich Soil Amendments Suppress Rhizoctonia solani Disease via Changes to the Soil Microbial Community.
33771785	3	24	theme	different	546:554	arg1	properties					572:581	different physicochemical properties	546:581	different physicochemical properties	546:581	To study which characteristics of organic products are correlated with disease suppression, an experiment was designed in which 10 types of organic amendments with different physicochemical properties were tested against the soilborne pathogen Rhizoctonia solani in sugar beet seedlings.
33771785	4	25	from	keratin	697:703	arg1	rich					689:692	rich	689:692	rich	689:692	Organic amendments rich in keratin or chitin reduced Rhizoctonia solani disease symptoms in sugar beet plants.
33771785	3	26	dep	pathogen	617:624	arg1	solani					638:643	solani	638:643	solani	638:643	To study which characteristics of organic products are correlated with disease suppression, an experiment was designed in which 10 types of organic amendments with different physicochemical properties were tested against the soilborne pathogen Rhizoctonia solani in sugar beet seedlings.
33771785	6	27	dep	known	1078:1082	arg1	Mortierellaceae					1164:1178	Mortierellaceae	1164:1178	Mortierellaceae	1164:1178	The Rhizoctonia-suppressive amended soils were rich in saprophytic bacteria and fungi that are known for their keratinolytic and chitinolytic properties (i.e., Oxalobacteraceae and Mortierellaceae).
33771785	6	27	dep	known	1078:1082	arg1	Oxalobacteraceae					1143:1158	Oxalobacteraceae	1143:1158	Oxalobacteraceae	1143:1158	The Rhizoctonia-suppressive amended soils were rich in saprophytic bacteria and fungi that are known for their keratinolytic and chitinolytic properties (i.e., Oxalobacteraceae and Mortierellaceae).
33771785	6	28	from	fungi	1063:1067	arg1	rich					1030:1033	rich	1030:1033	rich	1030:1033	The Rhizoctonia-suppressive amended soils were rich in saprophytic bacteria and fungi that are known for their keratinolytic and chitinolytic properties (i.e., Oxalobacteraceae and Mortierellaceae).
33771785	6	28	from	fungi	1063:1067	arg1	soils					1019:1023	The Rhizoctonia-suppressive amended soils	983:1023	The Rhizoctonia-suppressive amended soils	983:1023	The Rhizoctonia-suppressive amended soils were rich in saprophytic bacteria and fungi that are known for their keratinolytic and chitinolytic properties (i.e., Oxalobacteraceae and Mortierellaceae).
33771785	7	29	from	importance	1348:1357	arg1	suppression					1399:1409	plant disease suppression	1385:1409	plant disease suppression	1385:1409	The microbial community in keratin- and chitin-amended soils was associated with higher zinc, copper, and selenium, respectively.IMPORTANCE Our results highlight the importance of soil microorganisms in plant disease suppression and the possibility to steer soil microbial community composition by applying organic amendments to the soil.
33771785	2	30	theme	chitin	321:326	arg1	products					328:335	chitin products	321:335	chitin products the most efficient against fungal pathogens	321:379	Organic amendments have been shown to reduce crop diseases and pests, with chitin products the most efficient against fungal pathogens.
33771785	7	31	theme	chitin-amended	1222:1235	arg1	soils					1237:1241	keratin- and chitin-amended soils	1209:1241	keratin- and chitin-amended soils	1209:1241	The microbial community in keratin- and chitin-amended soils was associated with higher zinc, copper, and selenium, respectively.IMPORTANCE Our results highlight the importance of soil microorganisms in plant disease suppression and the possibility to steer soil microbial community composition by applying organic amendments to the soil.
33771785	7	32	theme	soil	1440:1443	arg1	composition					1465:1475	soil microbial community composition	1440:1475	soil microbial community composition	1440:1475	The microbial community in keratin- and chitin-amended soils was associated with higher zinc, copper, and selenium, respectively.IMPORTANCE Our results highlight the importance of soil microorganisms in plant disease suppression and the possibility to steer soil microbial community composition by applying organic amendments to the soil.
33771785	4	33	from	rich	689:692	arg1	keratin					697:703	keratin	697:703	keratin	697:703	Organic amendments rich in keratin or chitin reduced Rhizoctonia solani disease symptoms in sugar beet plants.
33771785	4	33	from	rich	689:692	arg1	chitin					708:713	chitin	708:713	chitin	708:713	Organic amendments rich in keratin or chitin reduced Rhizoctonia solani disease symptoms in sugar beet plants.
33771785	1	34	theme	promising	191:199	arg1	strategy					213:220	a promising alternative strategy	189:220	a promising alternative strategy to chemical pesticides	189:243	Enhancing soil suppressiveness against plant pathogens or pests is a promising alternative strategy to chemical pesticides.
33771785	1	34	theme	promising	191:199	arg1	suppressiveness					137:151	Enhancing soil suppressiveness	122:151	Enhancing soil suppressiveness against plant pathogens or pests	122:184	Enhancing soil suppressiveness against plant pathogens or pests is a promising alternative strategy to chemical pesticides.
33771785	0	35	theme	Soil	25:28	arg1	Amendments					30:39	Keratin-Rich Soil Amendments	12:39	Keratin-Rich Soil Amendments	12:39	Chitin- and Keratin-Rich Soil Amendments Suppress Rhizoctonia solani Disease via Changes to the Soil Microbial Community.
33771785	6	36	theme	keratinolytic	1094:1106	arg1	properties					1125:1134	their keratinolytic and chitinolytic properties	1088:1134	their keratinolytic and chitinolytic properties	1088:1134	The Rhizoctonia-suppressive amended soils were rich in saprophytic bacteria and fungi that are known for their keratinolytic and chitinolytic properties (i.e., Oxalobacteraceae and Mortierellaceae).
33771785	3	37	from	pathogen	617:624	arg1	seedlings					659:667	sugar beet seedlings	648:667	sugar beet seedlings	648:667	To study which characteristics of organic products are correlated with disease suppression, an experiment was designed in which 10 types of organic amendments with different physicochemical properties were tested against the soilborne pathogen Rhizoctonia solani in sugar beet seedlings.
33771785	1	38	theme	alternative	201:211	arg1	strategy					213:220	a promising alternative strategy	189:220	a promising alternative strategy to chemical pesticides	189:243	Enhancing soil suppressiveness against plant pathogens or pests is a promising alternative strategy to chemical pesticides.
33771785	1	38	theme	alternative	201:211	arg1	suppressiveness					137:151	Enhancing soil suppressiveness	122:151	Enhancing soil suppressiveness against plant pathogens or pests	122:184	Enhancing soil suppressiveness against plant pathogens or pests is a promising alternative strategy to chemical pesticides.
33771785	0	39	theme	Keratin-Rich	12:23	arg1	Amendments					30:39	Keratin-Rich Soil Amendments	12:39	Keratin-Rich Soil Amendments	12:39	Chitin- and Keratin-Rich Soil Amendments Suppress Rhizoctonia solani Disease via Changes to the Soil Microbial Community.
33771785	4	40	theme	Rhizoctonia	723:733	arg1	symptoms					750:757	Rhizoctonia solani disease symptoms	723:757	Rhizoctonia solani disease symptoms	723:757	Organic amendments rich in keratin or chitin reduced Rhizoctonia solani disease symptoms in sugar beet plants.
33771785	3	41	theme	products	424:431	arg1	characteristics					397:411	characteristics	397:411	characteristics of organic products	397:431	To study which characteristics of organic products are correlated with disease suppression, an experiment was designed in which 10 types of organic amendments with different physicochemical properties were tested against the soilborne pathogen Rhizoctonia solani in sugar beet seedlings.
33771785	5	42	theme	other	950:954	arg1	treatments					971:980	other nonsuppressive treatments	950:980	other nonsuppressive treatments	950:980	The bacterial and fungal microbial communities in amended soils were distinct from the microbial communities in nonamended soil, as well as those in soils that received other nonsuppressive treatments.
33771785	5	43	theme	nonamended	893:902	arg1	soil					904:907	nonamended soil	893:907	nonamended soil	893:907	The bacterial and fungal microbial communities in amended soils were distinct from the microbial communities in nonamended soil, as well as those in soils that received other nonsuppressive treatments.
33771785	4	44	theme	rich	689:692	arg1	amendments					678:687	Organic amendments	670:687	Organic amendments rich in keratin or chitin	670:713	Organic amendments rich in keratin or chitin reduced Rhizoctonia solani disease symptoms in sugar beet plants.
33771785	5	45	theme	amended	831:837	arg1	soils					839:843	amended soils	831:843	amended soils	831:843	The bacterial and fungal microbial communities in amended soils were distinct from the microbial communities in nonamended soil, as well as those in soils that received other nonsuppressive treatments.
33771785	6	46	theme	amended	1011:1017	arg1	rich					1030:1033	rich	1030:1033	rich	1030:1033	The Rhizoctonia-suppressive amended soils were rich in saprophytic bacteria and fungi that are known for their keratinolytic and chitinolytic properties (i.e., Oxalobacteraceae and Mortierellaceae).
33771785	6	46	theme	amended	1011:1017	arg1	soils					1019:1023	The Rhizoctonia-suppressive amended soils	983:1023	The Rhizoctonia-suppressive amended soils	983:1023	The Rhizoctonia-suppressive amended soils were rich in saprophytic bacteria and fungi that are known for their keratinolytic and chitinolytic properties (i.e., Oxalobacteraceae and Mortierellaceae).
33771785	6	47	from	rich	1030:1033	arg1	fungi					1063:1067	fungi	1063:1067	fungi	1063:1067	The Rhizoctonia-suppressive amended soils were rich in saprophytic bacteria and fungi that are known for their keratinolytic and chitinolytic properties (i.e., Oxalobacteraceae and Mortierellaceae).
33771785	6	47	from	rich	1030:1033	arg1	bacteria					1050:1057	bacteria	1050:1057	bacteria	1050:1057	The Rhizoctonia-suppressive amended soils were rich in saprophytic bacteria and fungi that are known for their keratinolytic and chitinolytic properties (i.e., Oxalobacteraceae and Mortierellaceae).
33771785	2	48	theme	Organic	246:252	arg1	amendments					254:263	Organic amendments	246:263	Organic amendments	246:263	Organic amendments have been shown to reduce crop diseases and pests, with chitin products the most efficient against fungal pathogens.
33771785	1	49	theme	chemical	225:232	arg1	pesticides					234:243	chemical pesticides	225:243	chemical pesticides	225:243	Enhancing soil suppressiveness against plant pathogens or pests is a promising alternative strategy to chemical pesticides.
33771785	0	50	theme	solani	62:67	arg1	Disease					69:75	Rhizoctonia solani Disease	50:75	Rhizoctonia solani Disease	50:75	Chitin- and Keratin-Rich Soil Amendments Suppress Rhizoctonia solani Disease via Changes to the Soil Microbial Community.
33771785	6	51	theme	Rhizoctonia-suppressive	987:1009	arg1	rich					1030:1033	rich	1030:1033	rich	1030:1033	The Rhizoctonia-suppressive amended soils were rich in saprophytic bacteria and fungi that are known for their keratinolytic and chitinolytic properties (i.e., Oxalobacteraceae and Mortierellaceae).
33771785	6	51	theme	Rhizoctonia-suppressive	987:1009	arg1	soils					1019:1023	The Rhizoctonia-suppressive amended soils	983:1023	The Rhizoctonia-suppressive amended soils	983:1023	The Rhizoctonia-suppressive amended soils were rich in saprophytic bacteria and fungi that are known for their keratinolytic and chitinolytic properties (i.e., Oxalobacteraceae and Mortierellaceae).
33771785	3	52	theme	soilborne	607:615	arg1	pathogen					617:624	the soilborne pathogen	603:624	the soilborne pathogen Rhizoctonia solani in sugar beet seedlings	603:667	To study which characteristics of organic products are correlated with disease suppression, an experiment was designed in which 10 types of organic amendments with different physicochemical properties were tested against the soilborne pathogen Rhizoctonia solani in sugar beet seedlings.
33771785	2	53	theme	efficient	346:354	arg1	products					328:335	chitin products	321:335	chitin products the most efficient against fungal pathogens	321:379	Organic amendments have been shown to reduce crop diseases and pests, with chitin products the most efficient against fungal pathogens.
33771785	0	54	theme	Rhizoctonia	50:60	arg1	Disease					69:75	Rhizoctonia solani Disease	50:75	Rhizoctonia solani Disease	50:75	Chitin- and Keratin-Rich Soil Amendments Suppress Rhizoctonia solani Disease via Changes to the Soil Microbial Community.
33771785	3	55	theme	organic	416:422	arg1	products					424:431	organic products	416:431	organic products	416:431	To study which characteristics of organic products are correlated with disease suppression, an experiment was designed in which 10 types of organic amendments with different physicochemical properties were tested against the soilborne pathogen Rhizoctonia solani in sugar beet seedlings.
33771785	5	56	from	communities	878:888	arg1	soil					904:907	nonamended soil	893:907	nonamended soil	893:907	The bacterial and fungal microbial communities in amended soils were distinct from the microbial communities in nonamended soil, as well as those in soils that received other nonsuppressive treatments.
33771785	5	56	from	communities	878:888	arg1	distinct					850:857	distinct	850:857	distinct	850:857	The bacterial and fungal microbial communities in amended soils were distinct from the microbial communities in nonamended soil, as well as those in soils that received other nonsuppressive treatments.
33771785	5	56	from	communities	878:888	arg1	communities					816:826	The bacterial and fungal microbial communities	781:826	The bacterial and fungal microbial communities in amended soils	781:843	The bacterial and fungal microbial communities in amended soils were distinct from the microbial communities in nonamended soil, as well as those in soils that received other nonsuppressive treatments.
33771785	1	57	theme	Enhancing	122:130	arg1	strategy					213:220	a promising alternative strategy	189:220	a promising alternative strategy to chemical pesticides	189:243	Enhancing soil suppressiveness against plant pathogens or pests is a promising alternative strategy to chemical pesticides.
33771785	1	57	theme	Enhancing	122:130	arg1	suppressiveness					137:151	Enhancing soil suppressiveness	122:151	Enhancing soil suppressiveness against plant pathogens or pests	122:184	Enhancing soil suppressiveness against plant pathogens or pests is a promising alternative strategy to chemical pesticides.
33771785	7	58	from	community	1196:1204	arg1	soils					1237:1241	keratin- and chitin-amended soils	1209:1241	keratin- and chitin-amended soils	1209:1241	The microbial community in keratin- and chitin-amended soils was associated with higher zinc, copper, and selenium, respectively.IMPORTANCE Our results highlight the importance of soil microorganisms in plant disease suppression and the possibility to steer soil microbial community composition by applying organic amendments to the soil.
33771785	7	59	theme	keratin-	1209:1216	arg1	soils					1237:1241	keratin- and chitin-amended soils	1209:1241	keratin- and chitin-amended soils	1209:1241	The microbial community in keratin- and chitin-amended soils was associated with higher zinc, copper, and selenium, respectively.IMPORTANCE Our results highlight the importance of soil microorganisms in plant disease suppression and the possibility to steer soil microbial community composition by applying organic amendments to the soil.
33771785	5	60	from	communities	816:826	arg1	soils					839:843	amended soils	831:843	amended soils	831:843	The bacterial and fungal microbial communities in amended soils were distinct from the microbial communities in nonamended soil, as well as those in soils that received other nonsuppressive treatments.
33771785	3	61	theme	beet	654:657	arg1	seedlings					659:667	sugar beet seedlings	648:667	sugar beet seedlings	648:667	To study which characteristics of organic products are correlated with disease suppression, an experiment was designed in which 10 types of organic amendments with different physicochemical properties were tested against the soilborne pathogen Rhizoctonia solani in sugar beet seedlings.
33771785	6	62	dep	Oxalobacteraceae	1143:1158	arg1	i.e.					1137:1140	i.e.	1137:1140	i.e.	1137:1140	The Rhizoctonia-suppressive amended soils were rich in saprophytic bacteria and fungi that are known for their keratinolytic and chitinolytic properties (i.e., Oxalobacteraceae and Mortierellaceae).
33771785	1	63	theme	soil	132:135	arg1	strategy					213:220	a promising alternative strategy	189:220	a promising alternative strategy to chemical pesticides	189:243	Enhancing soil suppressiveness against plant pathogens or pests is a promising alternative strategy to chemical pesticides.
33771785	1	63	theme	soil	132:135	arg1	suppressiveness					137:151	Enhancing soil suppressiveness	122:151	Enhancing soil suppressiveness against plant pathogens or pests	122:184	Enhancing soil suppressiveness against plant pathogens or pests is a promising alternative strategy to chemical pesticides.
33771785	7	64	theme	organic	1489:1495	arg1	amendments					1497:1506	organic amendments	1489:1506	organic amendments	1489:1506	The microbial community in keratin- and chitin-amended soils was associated with higher zinc, copper, and selenium, respectively.IMPORTANCE Our results highlight the importance of soil microorganisms in plant disease suppression and the possibility to steer soil microbial community composition by applying organic amendments to the soil.
33771785	7	65	theme	soil	1362:1365	arg1	microorganisms					1367:1380	soil microorganisms	1362:1380	soil microorganisms	1362:1380	The microbial community in keratin- and chitin-amended soils was associated with higher zinc, copper, and selenium, respectively.IMPORTANCE Our results highlight the importance of soil microorganisms in plant disease suppression and the possibility to steer soil microbial community composition by applying organic amendments to the soil.
33771785	5	66	theme	microbial	868:876	arg1	communities					878:888	the microbial communities	864:888	the microbial communities in nonamended soil	864:907	The bacterial and fungal microbial communities in amended soils were distinct from the microbial communities in nonamended soil, as well as those in soils that received other nonsuppressive treatments.
33771785	1	67	theme	plant	161:165	arg1	pathogens					167:175	plant pathogens	161:175	plant pathogens	161:175	Enhancing soil suppressiveness against plant pathogens or pests is a promising alternative strategy to chemical pesticides.
33771785	4	68	theme	beet	768:771	arg1	plants					773:778	sugar beet plants	762:778	sugar beet plants	762:778	Organic amendments rich in keratin or chitin reduced Rhizoctonia solani disease symptoms in sugar beet plants.
33771785	4	69	theme	Organic	670:676	arg1	amendments					678:687	Organic amendments	670:687	Organic amendments rich in keratin or chitin	670:713	Organic amendments rich in keratin or chitin reduced Rhizoctonia solani disease symptoms in sugar beet plants.
33771785	3	70	theme	organic	522:528	arg1	amendments					530:539	organic amendments	522:539	organic amendments with different physicochemical properties	522:581	To study which characteristics of organic products are correlated with disease suppression, an experiment was designed in which 10 types of organic amendments with different physicochemical properties were tested against the soilborne pathogen Rhizoctonia solani in sugar beet seedlings.
33771785	7	71	theme	plant	1385:1389	arg1	suppression					1399:1409	plant disease suppression	1385:1409	plant disease suppression	1385:1409	The microbial community in keratin- and chitin-amended soils was associated with higher zinc, copper, and selenium, respectively.IMPORTANCE Our results highlight the importance of soil microorganisms in plant disease suppression and the possibility to steer soil microbial community composition by applying organic amendments to the soil.
33771785	5	72	theme	fungal	799:804	arg1	distinct					850:857	distinct	850:857	distinct	850:857	The bacterial and fungal microbial communities in amended soils were distinct from the microbial communities in nonamended soil, as well as those in soils that received other nonsuppressive treatments.
33771785	5	72	theme	fungal	799:804	arg1	communities					816:826	The bacterial and fungal microbial communities	781:826	The bacterial and fungal microbial communities in amended soils	781:843	The bacterial and fungal microbial communities in amended soils were distinct from the microbial communities in nonamended soil, as well as those in soils that received other nonsuppressive treatments.
33771785	3	73	theme	sugar	648:652	arg1	seedlings					659:667	sugar beet seedlings	648:667	sugar beet seedlings	648:667	To study which characteristics of organic products are correlated with disease suppression, an experiment was designed in which 10 types of organic amendments with different physicochemical properties were tested against the soilborne pathogen Rhizoctonia solani in sugar beet seedlings.
33771785	3	74	theme	amendments	530:539	arg1	types					513:517	10 types	510:517	10 types of organic amendments with different physicochemical properties	510:581	To study which characteristics of organic products are correlated with disease suppression, an experiment was designed in which 10 types of organic amendments with different physicochemical properties were tested against the soilborne pathogen Rhizoctonia solani in sugar beet seedlings.
32090495	3	0	from	matrix	700:705	arg1	systems					724:730	drug delivery systems	710:730	drug delivery systems	710:730	The synthesized hydrogels are studied with respect to the specific characteristics of the gels: swelling kinetics, thermal analysis, viscoelastic characteristics, and their ability to be used as a matrix in drug delivery systems.
32090495	1	1	theme	study	159:163	arg1	purpose					144:150	The purpose	140:150	The purpose of the study	140:163	The purpose of the study is to obtain multicomponent polyelectrolyte hydrogels with optimal synergistic properties by combining a modified starch with a synthetic one.
32090495	1	2	theme	modified	270:277	arg1	starch					279:284	a modified starch	268:284	a modified starch	268:284	The purpose of the study is to obtain multicomponent polyelectrolyte hydrogels with optimal synergistic properties by combining a modified starch with a synthetic one.
32090495	0	3	theme	2-Dimethylaminoethyl	68:87	arg1	Poly					63:66	Poly	63:66	Poly(2-Dimethylaminoethyl Methacrylate)	63:101	Stimuli Responsive Scaffolds Based on Carboxymethyl Starch and Poly(2-Dimethylaminoethyl Methacrylate) for Anti-Inflammatory Drug Delivery.
32090495	0	3	theme	2-Dimethylaminoethyl	68:87	arg1	Methacrylate					89:100	2-Dimethylaminoethyl Methacrylate	68:100	2-Dimethylaminoethyl Methacrylate	68:100	Stimuli Responsive Scaffolds Based on Carboxymethyl Starch and Poly(2-Dimethylaminoethyl Methacrylate) for Anti-Inflammatory Drug Delivery.
32090495	3	4	theme	drug	710:713	arg1	systems					724:730	drug delivery systems	710:730	drug delivery systems	710:730	The synthesized hydrogels are studied with respect to the specific characteristics of the gels: swelling kinetics, thermal analysis, viscoelastic characteristics, and their ability to be used as a matrix in drug delivery systems.
32090495	6	5	theme	delivery	1142:1149	arg1	systems					1151:1157	drug delivery systems	1137:1157	drug delivery systems	1137:1157	The investigations confirm the obtainment of new semi-IPN hydrogels with pH and temperature responsiveness, good mechanical strength, and potential for use as drug delivery systems or transdermal patches.
32090495	3	6	theme	delivery	715:722	arg1	systems					724:730	drug delivery systems	710:730	drug delivery systems	710:730	The synthesized hydrogels are studied with respect to the specific characteristics of the gels: swelling kinetics, thermal analysis, viscoelastic characteristics, and their ability to be used as a matrix in drug delivery systems.
32090495	4	7	theme	drug	841:844	arg1	release					846:852	the in vitro drug release	828:852	the in vitro drug release	828:852	Therefore, the semi-IPN gels are loaded with ibuprofen, followed by additional tests to assess the in vitro drug release.
32090495	3	8	theme	gels	593:596	arg1	characteristics					570:584	the specific characteristics	557:584	the specific characteristics of the gels	557:596	The synthesized hydrogels are studied with respect to the specific characteristics of the gels: swelling kinetics, thermal analysis, viscoelastic characteristics, and their ability to be used as a matrix in drug delivery systems.
32090495	2	9	theme	semi-IPN	384:391	arg1	hydrogels					394:402	new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels	314:402	new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels of carboxymethyl starch and poly(2-dimethylaminoethyl methacrylate)	314:470	Thus, new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels of carboxymethyl starch and poly(2-dimethylaminoethyl methacrylate) are prepared and investigated.
32090495	0	10	theme	Drug	125:128	arg1	Delivery					130:137	Anti-Inflammatory Drug Delivery	107:137	Anti-Inflammatory Drug Delivery	107:137	Stimuli Responsive Scaffolds Based on Carboxymethyl Starch and Poly(2-Dimethylaminoethyl Methacrylate) for Anti-Inflammatory Drug Delivery.
32090495	3	11	theme	synthesized	507:517	arg1	hydrogels					519:527	The synthesized hydrogels	503:527	The synthesized hydrogels	503:527	The synthesized hydrogels are studied with respect to the specific characteristics of the gels: swelling kinetics, thermal analysis, viscoelastic characteristics, and their ability to be used as a matrix in drug delivery systems.
32090495	2	12	theme	carboxymethyl	407:419	arg1	starch					421:426	carboxymethyl starch	407:426	carboxymethyl starch	407:426	Thus, new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels of carboxymethyl starch and poly(2-dimethylaminoethyl methacrylate) are prepared and investigated.
32090495	0	13	theme	Anti-Inflammatory	107:123	arg1	Delivery					130:137	Anti-Inflammatory Drug Delivery	107:137	Anti-Inflammatory Drug Delivery	107:137	Stimuli Responsive Scaffolds Based on Carboxymethyl Starch and Poly(2-Dimethylaminoethyl Methacrylate) for Anti-Inflammatory Drug Delivery.
32090495	6	14	dep	pH	1051:1052	arg1	responsiveness					1070:1083	responsiveness	1070:1083	responsiveness	1070:1083	The investigations confirm the obtainment of new semi-IPN hydrogels with pH and temperature responsiveness, good mechanical strength, and potential for use as drug delivery systems or transdermal patches.
32090495	6	15	theme	drug	1137:1140	arg1	systems					1151:1157	drug delivery systems	1137:1157	drug delivery systems	1137:1157	The investigations confirm the obtainment of new semi-IPN hydrogels with pH and temperature responsiveness, good mechanical strength, and potential for use as drug delivery systems or transdermal patches.
32090495	2	16	theme	starch	421:426	arg1	hydrogels					394:402	new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels	314:402	new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels of carboxymethyl starch and poly(2-dimethylaminoethyl methacrylate)	314:470	Thus, new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels of carboxymethyl starch and poly(2-dimethylaminoethyl methacrylate) are prepared and investigated.
32090495	1	17	theme	multicomponent	178:191	arg1	hydrogels					209:217	multicomponent polyelectrolyte hydrogels	178:217	multicomponent polyelectrolyte hydrogels with optimal synergistic properties	178:253	The purpose of the study is to obtain multicomponent polyelectrolyte hydrogels with optimal synergistic properties by combining a modified starch with a synthetic one.
32090495	1	18	theme	synthetic	293:301	arg1	one					303:305	a synthetic one	291:305	a synthetic one	291:305	The purpose of the study is to obtain multicomponent polyelectrolyte hydrogels with optimal synergistic properties by combining a modified starch with a synthetic one.
32090495	2	19	theme	low-cost	318:325	arg1	hydrogels					394:402	new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels	314:402	new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels of carboxymethyl starch and poly(2-dimethylaminoethyl methacrylate)	314:470	Thus, new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels of carboxymethyl starch and poly(2-dimethylaminoethyl methacrylate) are prepared and investigated.
32090495	6	20	theme	new	1023:1025	arg1	hydrogels					1036:1044	new semi-IPN hydrogels	1023:1044	new semi-IPN hydrogels with pH and temperature responsiveness, good mechanical strength, and potential for use as drug delivery systems or transdermal patches	1023:1180	The investigations confirm the obtainment of new semi-IPN hydrogels with pH and temperature responsiveness, good mechanical strength, and potential for use as drug delivery systems or transdermal patches.
32090495	1	21	theme	polyelectrolyte	193:207	arg1	hydrogels					209:217	multicomponent polyelectrolyte hydrogels	178:217	multicomponent polyelectrolyte hydrogels with optimal synergistic properties	178:253	The purpose of the study is to obtain multicomponent polyelectrolyte hydrogels with optimal synergistic properties by combining a modified starch with a synthetic one.
32090495	2	22	theme	new	314:316	arg1	hydrogels					394:402	new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels	314:402	new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels of carboxymethyl starch and poly(2-dimethylaminoethyl methacrylate)	314:470	Thus, new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels of carboxymethyl starch and poly(2-dimethylaminoethyl methacrylate) are prepared and investigated.
32090495	6	23	with	hydrogels	1036:1044	arg1	strength					1102:1109	good mechanical strength	1086:1109	good mechanical strength	1086:1109	The investigations confirm the obtainment of new semi-IPN hydrogels with pH and temperature responsiveness, good mechanical strength, and potential for use as drug delivery systems or transdermal patches.
32090495	6	23	with	hydrogels	1036:1044	arg1	potential					1116:1124	potential	1116:1124	potential for use as drug delivery systems or transdermal patches	1116:1180	The investigations confirm the obtainment of new semi-IPN hydrogels with pH and temperature responsiveness, good mechanical strength, and potential for use as drug delivery systems or transdermal patches.
32090495	6	23	with	hydrogels	1036:1044	arg1	temperature					1058:1068	temperature	1058:1068	temperature	1058:1068	The investigations confirm the obtainment of new semi-IPN hydrogels with pH and temperature responsiveness, good mechanical strength, and potential for use as drug delivery systems or transdermal patches.
32090495	6	23	with	hydrogels	1036:1044	arg1	pH					1051:1052	pH	1051:1052	pH	1051:1052	The investigations confirm the obtainment of new semi-IPN hydrogels with pH and temperature responsiveness, good mechanical strength, and potential for use as drug delivery systems or transdermal patches.
32090495	5	24	theme	fibroblast	953:962	arg1	culture					969:975	fibroblast cell culture	953:975	fibroblast cell culture	953:975	The cytocompatibility of the hydrogels with respect to their composition is evaluated in vitro on fibroblast cell culture.
32090495	3	25	theme	swelling	599:606	arg1	kinetics					608:615	swelling kinetics	599:615	swelling kinetics	599:615	The synthesized hydrogels are studied with respect to the specific characteristics of the gels: swelling kinetics, thermal analysis, viscoelastic characteristics, and their ability to be used as a matrix in drug delivery systems.
32090495	2	26	theme	2-dimethylaminoethyl	437:456	arg1	poly					432:435	poly	432:435	poly(2-dimethylaminoethyl methacrylate)	432:470	Thus, new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels of carboxymethyl starch and poly(2-dimethylaminoethyl methacrylate) are prepared and investigated.
32090495	2	26	theme	2-dimethylaminoethyl	437:456	arg1	methacrylate					458:469	2-dimethylaminoethyl methacrylate	437:469	2-dimethylaminoethyl methacrylate	437:469	Thus, new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels of carboxymethyl starch and poly(2-dimethylaminoethyl methacrylate) are prepared and investigated.
32090495	0	27	theme	Carboxymethyl	38:50	arg1	Starch					52:57	Carboxymethyl Starch	38:57	Carboxymethyl Starch	38:57	Stimuli Responsive Scaffolds Based on Carboxymethyl Starch and Poly(2-Dimethylaminoethyl Methacrylate) for Anti-Inflammatory Drug Delivery.
32090495	4	28	dep	loaded	766:771	arg1	followed					789:796	followed	789:796	followed by additional tests to assess the in vitro drug release	789:852	Therefore, the semi-IPN gels are loaded with ibuprofen, followed by additional tests to assess the in vitro drug release.
32090495	1	29	with	hydrogels	209:217	arg1	properties					244:253	optimal synergistic properties	224:253	optimal synergistic properties	224:253	The purpose of the study is to obtain multicomponent polyelectrolyte hydrogels with optimal synergistic properties by combining a modified starch with a synthetic one.
32090495	4	30	dep	in	832:833	arg1	vitro					835:839	vitro	835:839	vitro	835:839	Therefore, the semi-IPN gels are loaded with ibuprofen, followed by additional tests to assess the in vitro drug release.
32090495	4	31	theme	in	832:833	arg1	release					846:852	the in vitro drug release	828:852	the in vitro drug release	828:852	Therefore, the semi-IPN gels are loaded with ibuprofen, followed by additional tests to assess the in vitro drug release.
32090495	6	32	theme	good	1086:1089	arg1	strength					1102:1109	good mechanical strength	1086:1109	good mechanical strength	1086:1109	The investigations confirm the obtainment of new semi-IPN hydrogels with pH and temperature responsiveness, good mechanical strength, and potential for use as drug delivery systems or transdermal patches.
32090495	1	33	theme	optimal	224:230	arg1	properties					244:253	optimal synergistic properties	224:253	optimal synergistic properties	224:253	The purpose of the study is to obtain multicomponent polyelectrolyte hydrogels with optimal synergistic properties by combining a modified starch with a synthetic one.
32090495	6	34	theme	mechanical	1091:1100	arg1	strength					1102:1109	good mechanical strength	1086:1109	good mechanical strength	1086:1109	The investigations confirm the obtainment of new semi-IPN hydrogels with pH and temperature responsiveness, good mechanical strength, and potential for use as drug delivery systems or transdermal patches.
32090495	5	35	theme	hydrogels	884:892	arg1	cytocompatibility					859:875	The cytocompatibility	855:875	The cytocompatibility of the hydrogels with respect to their composition	855:926	The cytocompatibility of the hydrogels with respect to their composition is evaluated in vitro on fibroblast cell culture.
32090495	3	36	theme	viscoelastic	636:647	arg1	characteristics					649:663	viscoelastic characteristics	636:663	viscoelastic characteristics	636:663	The synthesized hydrogels are studied with respect to the specific characteristics of the gels: swelling kinetics, thermal analysis, viscoelastic characteristics, and their ability to be used as a matrix in drug delivery systems.
32090495	1	37	theme	synergistic	232:242	arg1	properties					244:253	optimal synergistic properties	224:253	optimal synergistic properties	224:253	The purpose of the study is to obtain multicomponent polyelectrolyte hydrogels with optimal synergistic properties by combining a modified starch with a synthetic one.
32090495	3	38	theme	thermal	618:624	arg1	analysis					626:633	thermal analysis	618:633	thermal analysis	618:633	The synthesized hydrogels are studied with respect to the specific characteristics of the gels: swelling kinetics, thermal analysis, viscoelastic characteristics, and their ability to be used as a matrix in drug delivery systems.
32090495	2	39	theme	network	375:381	arg1	hydrogels					394:402	new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels	314:402	new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels of carboxymethyl starch and poly(2-dimethylaminoethyl methacrylate)	314:470	Thus, new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels of carboxymethyl starch and poly(2-dimethylaminoethyl methacrylate) are prepared and investigated.
32090495	6	40	theme	transdermal	1162:1172	arg1	patches					1174:1180	transdermal patches	1162:1180	transdermal patches	1162:1180	The investigations confirm the obtainment of new semi-IPN hydrogels with pH and temperature responsiveness, good mechanical strength, and potential for use as drug delivery systems or transdermal patches.
32090495	2	41	theme	polymer	367:373	arg1	hydrogels					394:402	new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels	314:402	new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels of carboxymethyl starch and poly(2-dimethylaminoethyl methacrylate)	314:470	Thus, new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels of carboxymethyl starch and poly(2-dimethylaminoethyl methacrylate) are prepared and investigated.
32090495	2	42	theme	semi-interpenetrating	345:365	arg1	hydrogels					394:402	new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels	314:402	new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels of carboxymethyl starch and poly(2-dimethylaminoethyl methacrylate)	314:470	Thus, new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels of carboxymethyl starch and poly(2-dimethylaminoethyl methacrylate) are prepared and investigated.
32090495	5	43	theme	cell	964:967	arg1	culture					969:975	fibroblast cell culture	953:975	fibroblast cell culture	953:975	The cytocompatibility of the hydrogels with respect to their composition is evaluated in vitro on fibroblast cell culture.
32090495	4	44	theme	additional	801:810	arg1	tests					812:816	additional tests	801:816	additional tests	801:816	Therefore, the semi-IPN gels are loaded with ibuprofen, followed by additional tests to assess the in vitro drug release.
32090495	2	45	theme	biocompatible	331:343	arg1	hydrogels					394:402	new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels	314:402	new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels of carboxymethyl starch and poly(2-dimethylaminoethyl methacrylate)	314:470	Thus, new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels of carboxymethyl starch and poly(2-dimethylaminoethyl methacrylate) are prepared and investigated.
32090495	4	46	theme	semi-IPN	748:755	arg1	gels					757:760	the semi-IPN gels	744:760	the semi-IPN gels	744:760	Therefore, the semi-IPN gels are loaded with ibuprofen, followed by additional tests to assess the in vitro drug release.
32090495	2	47	theme	poly	432:435	arg1	hydrogels					394:402	new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels	314:402	new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels of carboxymethyl starch and poly(2-dimethylaminoethyl methacrylate)	314:470	Thus, new low-cost and biocompatible semi-interpenetrating polymer network (semi-IPN) hydrogels of carboxymethyl starch and poly(2-dimethylaminoethyl methacrylate) are prepared and investigated.
32090495	6	48	theme	hydrogels	1036:1044	arg1	obtainment					1009:1018	the obtainment	1005:1018	the obtainment of new semi-IPN hydrogels with pH and temperature responsiveness, good mechanical strength, and potential for use as drug delivery systems or transdermal patches	1005:1180	The investigations confirm the obtainment of new semi-IPN hydrogels with pH and temperature responsiveness, good mechanical strength, and potential for use as drug delivery systems or transdermal patches.
32090495	3	49	theme	specific	561:568	arg1	characteristics					570:584	the specific characteristics	557:584	the specific characteristics of the gels	557:596	The synthesized hydrogels are studied with respect to the specific characteristics of the gels: swelling kinetics, thermal analysis, viscoelastic characteristics, and their ability to be used as a matrix in drug delivery systems.
32090495	6	50	theme	semi-IPN	1027:1034	arg1	hydrogels					1036:1044	new semi-IPN hydrogels	1023:1044	new semi-IPN hydrogels with pH and temperature responsiveness, good mechanical strength, and potential for use as drug delivery systems or transdermal patches	1023:1180	The investigations confirm the obtainment of new semi-IPN hydrogels with pH and temperature responsiveness, good mechanical strength, and potential for use as drug delivery systems or transdermal patches.
32516027	5	0	dep	groups	792:797	arg1	wk					802:803	6 wk	800:803	6 wk treadmill-based ET	800:822	Mice were divided into sedentary (Sed) or ET groups (6 wk treadmill-based ET) while maintaining their diets, resulting in four donor groups: chow sedentary (NC-Sed) or ET (NC-ET) and HFHS sedentary (HFHS-Sed) or ET (HFHS-ET).
32516027	7	1	from	intolerance	1207:1217	arg1	donors					1238:1243	the donors	1234:1243	the donors	1234:1243	The HFHS diet led to glucose intolerance and obesity in the donors, whereas exercise training (ET) restrained adiposity and improved glucose tolerance.
32516027	9	2	theme	donor-ET	1522:1529	arg1	status					1531:1536	donor-ET status	1522:1536	donor-ET status	1522:1536	Despite unaltered adiposity, glucose levels were disrupted when challenged in mice receiving feces from HFHS-fed donors, irrespective of donor-ET status, with a decrease in insulin-stimulated glucose clearance into white adipose tissue and large intestine and specific changes in the recipient's microbiota composition observed.
32516027	11	3	theme	protective	1846:1855	arg1	effect					1867:1872	the protective metabolic effect	1842:1872	the protective metabolic effect of ET on glucose metabolism	1842:1900	However, the protective metabolic effect of ET on glucose metabolism is not mediated through fecal factors.
32516027	4	4	theme	Donor	621:625	arg1	mice					636:639	Donor C57BL/6J mice	621:639	Donor C57BL/6J mice	621:639	Donor C57BL/6J mice were fed a chow or high-fat, high-sucrose diet (HFHS) for 4 wk to induce obesity and glucose intolerance.
32516027	1	5	dep	suggest	162:168	arg1	contributes					189:199	contributes	189:199	suggest the gut microbiota contributes to the development of obesity and metabolic syndrome	162:252	Studies suggest the gut microbiota contributes to the development of obesity and metabolic syndrome.
32516027	6	6	theme	body	1131:1134	arg1	composition					1136:1146	body composition	1131:1146	body composition	1131:1146	Chow-fed recipient mice were gavaged with feces from the respective donor groups weekly, creating four groups (NC-Sed-R, NC-ET-R, HFHS-Sed-R, HFHS-ET-R), and body composition and metabolism were assessed.
32516027	5	7	theme	ET	789:790	arg1	groups					792:797	sedentary (Sed) or ET groups	770:797	sedentary (Sed) or ET groups (6 wk treadmill-based ET)	770:823	Mice were divided into sedentary (Sed) or ET groups (6 wk treadmill-based ET) while maintaining their diets, resulting in four donor groups: chow sedentary (NC-Sed) or ET (NC-ET) and HFHS sedentary (HFHS-Sed) or ET (HFHS-ET).
32516027	10	8	from	change	1812:1817	arg1	adiposity					1822:1830	adiposity	1822:1830	adiposity	1822:1830	FMT can transmit HFHS-induced disrupted glucose metabolism to recipient mice independently of any change in adiposity.
32516027	5	9	dep	sedentary	770:778	arg1	Sed					781:783	Sed	781:783	Sed	781:783	Mice were divided into sedentary (Sed) or ET groups (6 wk treadmill-based ET) while maintaining their diets, resulting in four donor groups: chow sedentary (NC-Sed) or ET (NC-ET) and HFHS sedentary (HFHS-Sed) or ET (HFHS-ET).
32516027	5	10	dep	sedentary	935:943	arg1	HFHS-Sed					946:953	HFHS-Sed	946:953	HFHS-Sed	946:953	Mice were divided into sedentary (Sed) or ET groups (6 wk treadmill-based ET) while maintaining their diets, resulting in four donor groups: chow sedentary (NC-Sed) or ET (NC-ET) and HFHS sedentary (HFHS-Sed) or ET (HFHS-ET).
32516027	9	11	theme	glucose	1577:1583	arg1	clearance					1585:1593	insulin-stimulated glucose clearance	1558:1593	insulin-stimulated glucose clearance into white adipose tissue and large intestine	1558:1639	Despite unaltered adiposity, glucose levels were disrupted when challenged in mice receiving feces from HFHS-fed donors, irrespective of donor-ET status, with a decrease in insulin-stimulated glucose clearance into white adipose tissue and large intestine and specific changes in the recipient's microbiota composition observed.
32516027	11	12	theme	metabolic	1857:1865	arg1	effect					1867:1872	the protective metabolic effect	1842:1872	the protective metabolic effect of ET on glucose metabolism	1842:1900	However, the protective metabolic effect of ET on glucose metabolism is not mediated through fecal factors.
32516027	9	13	from	decrease	1546:1553	arg1	clearance					1585:1593	insulin-stimulated glucose clearance	1558:1593	insulin-stimulated glucose clearance into white adipose tissue and large intestine	1558:1639	Despite unaltered adiposity, glucose levels were disrupted when challenged in mice receiving feces from HFHS-fed donors, irrespective of donor-ET status, with a decrease in insulin-stimulated glucose clearance into white adipose tissue and large intestine and specific changes in the recipient's microbiota composition observed.
32516027	9	13	from	decrease	1546:1553	arg1	composition					1692:1702	the recipient's microbiota composition	1665:1702	the recipient's microbiota composition	1665:1702	Despite unaltered adiposity, glucose levels were disrupted when challenged in mice receiving feces from HFHS-fed donors, irrespective of donor-ET status, with a decrease in insulin-stimulated glucose clearance into white adipose tissue and large intestine and specific changes in the recipient's microbiota composition observed.
32516027	4	14	theme	high-fat	660:667	arg1	HFHS					689:692	HFHS	689:692	HFHS	689:692	Donor C57BL/6J mice were fed a chow or high-fat, high-sucrose diet (HFHS) for 4 wk to induce obesity and glucose intolerance.
32516027	4	14	theme	high-fat	660:667	arg1	diet					683:686	high-fat, high-sucrose diet	660:686	diet	683:686	Donor C57BL/6J mice were fed a chow or high-fat, high-sucrose diet (HFHS) for 4 wk to induce obesity and glucose intolerance.
32516027	0	15	theme	exercise	137:144	arg1	status					146:151	exercise status	137:151	exercise status	137:151	Fecal microbiota transplantation from high caloric-fed donors alters glucose metabolism in recipient mice, independently of adiposity or exercise status.
32516027	1	16	theme	obesity	223:229	arg1	development					208:218	the development	204:218	the development of obesity and metabolic syndrome	204:252	Studies suggest the gut microbiota contributes to the development of obesity and metabolic syndrome.
32516027	9	17	theme	adipose	1606:1612	arg1	tissue					1614:1619	white adipose tissue	1600:1619	white adipose tissue	1600:1619	Despite unaltered adiposity, glucose levels were disrupted when challenged in mice receiving feces from HFHS-fed donors, irrespective of donor-ET status, with a decrease in insulin-stimulated glucose clearance into white adipose tissue and large intestine and specific changes in the recipient's microbiota composition observed.
32516027	8	18	theme	donor	1333:1337	arg1	FMT					1345:1347	No donor group FMT	1330:1347	No donor group FMT	1330:1347	No donor group FMT altered recipient body composition.
32516027	5	19	theme	donor	874:878	arg1	groups					880:885	four donor groups	869:885	four donor groups: chow sedentary (NC-Sed) or ET (NC-ET) and HFHS sedentary (HFHS-Sed) or ET (HFHS-ET)	869:970	Mice were divided into sedentary (Sed) or ET groups (6 wk treadmill-based ET) while maintaining their diets, resulting in four donor groups: chow sedentary (NC-Sed) or ET (NC-ET) and HFHS sedentary (HFHS-Sed) or ET (HFHS-ET).
32516027	11	20	theme	glucose	1883:1889	arg1	metabolism					1891:1900	glucose metabolism	1883:1900	glucose metabolism	1883:1900	However, the protective metabolic effect of ET on glucose metabolism is not mediated through fecal factors.
32516027	2	21	theme	microbiota	271:280	arg1	composition					282:292	microbiota composition	271:292	microbiota composition	271:292	Exercise alters microbiota composition and diversity and is protective of these maladies.
32516027	0	22	theme	glucose	69:75	arg1	metabolism					77:86	glucose metabolism	69:86	glucose metabolism	69:86	Fecal microbiota transplantation from high caloric-fed donors alters glucose metabolism in recipient mice, independently of adiposity or exercise status.
32516027	9	23	theme	unaltered	1393:1401	arg1	adiposity					1403:1411	unaltered adiposity	1393:1411	unaltered adiposity	1393:1411	Despite unaltered adiposity, glucose levels were disrupted when challenged in mice receiving feces from HFHS-fed donors, irrespective of donor-ET status, with a decrease in insulin-stimulated glucose clearance into white adipose tissue and large intestine and specific changes in the recipient's microbiota composition observed.
32516027	9	24	theme	specific	1645:1652	arg1	changes					1654:1660	specific changes	1645:1660	specific changes in the recipient's microbiota composition observed	1645:1711	Despite unaltered adiposity, glucose levels were disrupted when challenged in mice receiving feces from HFHS-fed donors, irrespective of donor-ET status, with a decrease in insulin-stimulated glucose clearance into white adipose tissue and large intestine and specific changes in the recipient's microbiota composition observed.
32516027	8	25	theme	recipient	1357:1365	arg1	composition					1372:1382	recipient body composition	1357:1382	recipient body composition	1357:1382	No donor group FMT altered recipient body composition.
32516027	3	26	theme	metabolic	378:386	arg1	effects					388:394	the protective metabolic effects	363:394	the protective metabolic effects of exercise	363:406	We tested whether the protective metabolic effects of exercise are mediated through fecal components through assessment of body composition and metabolism in recipients of fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets.
32516027	5	27	dep	groups	880:885	arg1	NC-ET					919:923	NC-ET	919:923	NC-ET	919:923	Mice were divided into sedentary (Sed) or ET groups (6 wk treadmill-based ET) while maintaining their diets, resulting in four donor groups: chow sedentary (NC-Sed) or ET (NC-ET) and HFHS sedentary (HFHS-Sed) or ET (HFHS-ET).
32516027	5	27	dep	groups	880:885	arg1	HFHS-ET					963:969	HFHS-ET	963:969	HFHS-ET	963:969	Mice were divided into sedentary (Sed) or ET groups (6 wk treadmill-based ET) while maintaining their diets, resulting in four donor groups: chow sedentary (NC-Sed) or ET (NC-ET) and HFHS sedentary (HFHS-Sed) or ET (HFHS-ET).
32516027	5	27	dep	groups	880:885	arg1	ET					915:916	ET	915:916	ET (NC-ET)	915:924	Mice were divided into sedentary (Sed) or ET groups (6 wk treadmill-based ET) while maintaining their diets, resulting in four donor groups: chow sedentary (NC-Sed) or ET (NC-ET) and HFHS sedentary (HFHS-Sed) or ET (HFHS-ET).
32516027	5	27	dep	groups	880:885	arg1	sedentary					893:901	sedentary	893:901	sedentary	893:901	Mice were divided into sedentary (Sed) or ET groups (6 wk treadmill-based ET) while maintaining their diets, resulting in four donor groups: chow sedentary (NC-Sed) or ET (NC-ET) and HFHS sedentary (HFHS-Sed) or ET (HFHS-ET).
32516027	0	28	from	donors	55:60	arg1	transplantation					17:31	Fecal microbiota transplantation	0:31	Fecal microbiota transplantation from high caloric-fed donors	0:60	Fecal microbiota transplantation from high caloric-fed donors alters glucose metabolism in recipient mice, independently of adiposity or exercise status.
32516027	3	29	theme	body	468:471	arg1	composition					473:483	body composition	468:483	body composition	468:483	We tested whether the protective metabolic effects of exercise are mediated through fecal components through assessment of body composition and metabolism in recipients of fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets.
32516027	0	30	theme	caloric-fed	43:53	arg1	donors					55:60	high caloric-fed donors	38:60	high caloric-fed donors	38:60	Fecal microbiota transplantation from high caloric-fed donors alters glucose metabolism in recipient mice, independently of adiposity or exercise status.
32516027	9	31	from	donors	1498:1503	arg1	feces					1478:1482	feces	1478:1482	feces from HFHS-fed donors	1478:1503	Despite unaltered adiposity, glucose levels were disrupted when challenged in mice receiving feces from HFHS-fed donors, irrespective of donor-ET status, with a decrease in insulin-stimulated glucose clearance into white adipose tissue and large intestine and specific changes in the recipient's microbiota composition observed.
32516027	6	32	theme	Chow-fed	973:980	arg1	mice					992:995	Chow-fed recipient mice	973:995	Chow-fed recipient mice	973:995	Chow-fed recipient mice were gavaged with feces from the respective donor groups weekly, creating four groups (NC-Sed-R, NC-ET-R, HFHS-Sed-R, HFHS-ET-R), and body composition and metabolism were assessed.
32516027	9	33	theme	microbiota	1681:1690	arg1	composition					1692:1702	the recipient's microbiota composition	1665:1702	the recipient's microbiota composition	1665:1702	Despite unaltered adiposity, glucose levels were disrupted when challenged in mice receiving feces from HFHS-fed donors, irrespective of donor-ET status, with a decrease in insulin-stimulated glucose clearance into white adipose tissue and large intestine and specific changes in the recipient's microbiota composition observed.
32516027	4	34	theme	glucose	726:732	arg1	intolerance					734:744	glucose intolerance	726:744	glucose intolerance	726:744	Donor C57BL/6J mice were fed a chow or high-fat, high-sucrose diet (HFHS) for 4 wk to induce obesity and glucose intolerance.
32516027	0	35	theme	Fecal	0:4	arg1	transplantation					17:31	Fecal microbiota transplantation	0:31	Fecal microbiota transplantation from high caloric-fed donors	0:60	Fecal microbiota transplantation from high caloric-fed donors alters glucose metabolism in recipient mice, independently of adiposity or exercise status.
32516027	3	36	dep	mice	583:586	arg1	ET					579:580	ET	579:580	ET	579:580	We tested whether the protective metabolic effects of exercise are mediated through fecal components through assessment of body composition and metabolism in recipients of fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets.
32516027	0	37	theme	high	38:41	arg1	donors					55:60	high caloric-fed donors	38:60	high caloric-fed donors	38:60	Fecal microbiota transplantation from high caloric-fed donors alters glucose metabolism in recipient mice, independently of adiposity or exercise status.
32516027	10	38	theme	disrupted	1744:1752	arg1	metabolism					1762:1771	HFHS-induced disrupted glucose metabolism	1731:1771	HFHS-induced disrupted glucose metabolism	1731:1771	FMT can transmit HFHS-induced disrupted glucose metabolism to recipient mice independently of any change in adiposity.
32516027	3	39	theme	microbiota	523:532	arg1	FMT					551:553	FMT	551:553	FMT	551:553	We tested whether the protective metabolic effects of exercise are mediated through fecal components through assessment of body composition and metabolism in recipients of fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets.
32516027	3	39	theme	microbiota	523:532	arg1	transplantation					534:548	fecal microbiota transplantation	517:548	fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets	517:618	We tested whether the protective metabolic effects of exercise are mediated through fecal components through assessment of body composition and metabolism in recipients of fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets.
32516027	9	40	theme	HFHS-fed	1489:1496	arg1	donors					1498:1503	HFHS-fed donors	1489:1503	HFHS-fed donors	1489:1503	Despite unaltered adiposity, glucose levels were disrupted when challenged in mice receiving feces from HFHS-fed donors, irrespective of donor-ET status, with a decrease in insulin-stimulated glucose clearance into white adipose tissue and large intestine and specific changes in the recipient's microbiota composition observed.
32516027	5	41	dep	treadmill-based	805:819	arg1	ET					821:822	ET	821:822	ET	821:822	Mice were divided into sedentary (Sed) or ET groups (6 wk treadmill-based ET) while maintaining their diets, resulting in four donor groups: chow sedentary (NC-Sed) or ET (NC-ET) and HFHS sedentary (HFHS-Sed) or ET (HFHS-ET).
32516027	3	42	from	recipients	503:512	arg1	mice					583:586	exercise-trained (ET) mice	561:586	exercise-trained (ET) mice fed normal or high-energy diets	561:618	We tested whether the protective metabolic effects of exercise are mediated through fecal components through assessment of body composition and metabolism in recipients of fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets.
32516027	4	43	dep	diet	683:686	arg1	high-sucrose					670:681	high-fat, high-sucrose diet	660:686	high-sucrose	670:681	Donor C57BL/6J mice were fed a chow or high-fat, high-sucrose diet (HFHS) for 4 wk to induce obesity and glucose intolerance.
32516027	4	44	theme	C57BL/6J	627:634	arg1	mice					636:639	Donor C57BL/6J mice	621:639	Donor C57BL/6J mice	621:639	Donor C57BL/6J mice were fed a chow or high-fat, high-sucrose diet (HFHS) for 4 wk to induce obesity and glucose intolerance.
32516027	10	45	theme	recipient	1776:1784	arg1	mice					1786:1789	recipient mice	1776:1789	recipient mice	1776:1789	FMT can transmit HFHS-induced disrupted glucose metabolism to recipient mice independently of any change in adiposity.
32516027	1	46	theme	syndrome	245:252	arg1	development					208:218	the development	204:218	the development of obesity and metabolic syndrome	204:252	Studies suggest the gut microbiota contributes to the development of obesity and metabolic syndrome.
32516027	3	47	theme	normal	592:597	arg1	diets					614:618	normal or high-energy diets	592:618	normal or high-energy diets	592:618	We tested whether the protective metabolic effects of exercise are mediated through fecal components through assessment of body composition and metabolism in recipients of fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets.
32516027	3	48	theme	exercise-trained	561:576	arg1	mice					583:586	exercise-trained (ET) mice	561:586	exercise-trained (ET) mice fed normal or high-energy diets	561:618	We tested whether the protective metabolic effects of exercise are mediated through fecal components through assessment of body composition and metabolism in recipients of fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets.
32516027	1	49	theme	gut	174:176	arg1	microbiota					178:187	the gut microbiota	170:187	the gut microbiota	170:187	Studies suggest the gut microbiota contributes to the development of obesity and metabolic syndrome.
32516027	7	50	theme	glucose	1311:1317	arg1	tolerance					1319:1327	glucose tolerance	1311:1327	glucose tolerance	1311:1327	The HFHS diet led to glucose intolerance and obesity in the donors, whereas exercise training (ET) restrained adiposity and improved glucose tolerance.
32516027	2	51	theme	maladies	335:342	arg1	protective					315:324	protective	315:324	protective	315:324	Exercise alters microbiota composition and diversity and is protective of these maladies.
32516027	9	52	theme	insulin-stimulated	1558:1575	arg1	clearance					1585:1593	insulin-stimulated glucose clearance	1558:1593	insulin-stimulated glucose clearance into white adipose tissue and large intestine	1558:1639	Despite unaltered adiposity, glucose levels were disrupted when challenged in mice receiving feces from HFHS-fed donors, irrespective of donor-ET status, with a decrease in insulin-stimulated glucose clearance into white adipose tissue and large intestine and specific changes in the recipient's microbiota composition observed.
32516027	11	53	theme	fecal	1926:1930	arg1	factors					1932:1938	fecal factors	1926:1938	fecal factors	1926:1938	However, the protective metabolic effect of ET on glucose metabolism is not mediated through fecal factors.
32516027	6	54	theme	donor	1041:1045	arg1	groups					1047:1052	the respective donor groups	1026:1052	the respective donor groups	1026:1052	Chow-fed recipient mice were gavaged with feces from the respective donor groups weekly, creating four groups (NC-Sed-R, NC-ET-R, HFHS-Sed-R, HFHS-ET-R), and body composition and metabolism were assessed.
32516027	11	55	from	effect	1867:1872	arg1	metabolism					1891:1900	glucose metabolism	1883:1900	glucose metabolism	1883:1900	However, the protective metabolic effect of ET on glucose metabolism is not mediated through fecal factors.
32516027	5	56	theme	treadmill-based	805:819	arg1	wk					802:803	6 wk	800:803	6 wk treadmill-based ET	800:822	Mice were divided into sedentary (Sed) or ET groups (6 wk treadmill-based ET) while maintaining their diets, resulting in four donor groups: chow sedentary (NC-Sed) or ET (NC-ET) and HFHS sedentary (HFHS-Sed) or ET (HFHS-ET).
32516027	9	57	theme	white	1600:1604	arg1	tissue					1614:1619	white adipose tissue	1600:1619	white adipose tissue	1600:1619	Despite unaltered adiposity, glucose levels were disrupted when challenged in mice receiving feces from HFHS-fed donors, irrespective of donor-ET status, with a decrease in insulin-stimulated glucose clearance into white adipose tissue and large intestine and specific changes in the recipient's microbiota composition observed.
32516027	7	58	theme	exercise	1254:1261	arg1	training					1263:1270	exercise training	1254:1270	exercise training (ET)	1254:1275	The HFHS diet led to glucose intolerance and obesity in the donors, whereas exercise training (ET) restrained adiposity and improved glucose tolerance.
32516027	6	59	dep	groups	1076:1081	arg1	NC-ET-R					1094:1100	NC-ET-R	1094:1100	NC-ET-R	1094:1100	Chow-fed recipient mice were gavaged with feces from the respective donor groups weekly, creating four groups (NC-Sed-R, NC-ET-R, HFHS-Sed-R, HFHS-ET-R), and body composition and metabolism were assessed.
32516027	6	59	dep	groups	1076:1081	arg1	NC-Sed-R					1084:1091	NC-Sed-R	1084:1091	NC-Sed-R	1084:1091	Chow-fed recipient mice were gavaged with feces from the respective donor groups weekly, creating four groups (NC-Sed-R, NC-ET-R, HFHS-Sed-R, HFHS-ET-R), and body composition and metabolism were assessed.
32516027	6	59	dep	groups	1076:1081	arg1	HFHS-ET-R					1115:1123	HFHS-ET-R	1115:1123	HFHS-ET-R	1115:1123	Chow-fed recipient mice were gavaged with feces from the respective donor groups weekly, creating four groups (NC-Sed-R, NC-ET-R, HFHS-Sed-R, HFHS-ET-R), and body composition and metabolism were assessed.
32516027	6	59	dep	groups	1076:1081	arg1	HFHS-Sed-R					1103:1112	HFHS-Sed-R	1103:1112	HFHS-Sed-R	1103:1112	Chow-fed recipient mice were gavaged with feces from the respective donor groups weekly, creating four groups (NC-Sed-R, NC-ET-R, HFHS-Sed-R, HFHS-ET-R), and body composition and metabolism were assessed.
32516027	3	60	theme	high-energy	602:612	arg1	diets					614:618	normal or high-energy diets	592:618	normal or high-energy diets	592:618	We tested whether the protective metabolic effects of exercise are mediated through fecal components through assessment of body composition and metabolism in recipients of fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets.
32516027	8	61	theme	group	1339:1343	arg1	FMT					1345:1347	No donor group FMT	1330:1347	No donor group FMT	1330:1347	No donor group FMT altered recipient body composition.
32516027	3	62	from	mice	583:586	arg1	FMT					551:553	FMT	551:553	FMT	551:553	We tested whether the protective metabolic effects of exercise are mediated through fecal components through assessment of body composition and metabolism in recipients of fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets.
32516027	3	62	from	mice	583:586	arg1	transplantation					534:548	fecal microbiota transplantation	517:548	fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets	517:618	We tested whether the protective metabolic effects of exercise are mediated through fecal components through assessment of body composition and metabolism in recipients of fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets.
32516027	3	62	from	mice	583:586	arg1	recipients					503:512	recipients	503:512	recipients of fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets	503:618	We tested whether the protective metabolic effects of exercise are mediated through fecal components through assessment of body composition and metabolism in recipients of fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets.
32516027	10	63	theme	HFHS-induced	1731:1742	arg1	metabolism					1762:1771	HFHS-induced disrupted glucose metabolism	1731:1771	HFHS-induced disrupted glucose metabolism	1731:1771	FMT can transmit HFHS-induced disrupted glucose metabolism to recipient mice independently of any change in adiposity.
32516027	9	64	theme	large	1625:1629	arg1	intestine					1631:1639	large intestine	1625:1639	large intestine	1625:1639	Despite unaltered adiposity, glucose levels were disrupted when challenged in mice receiving feces from HFHS-fed donors, irrespective of donor-ET status, with a decrease in insulin-stimulated glucose clearance into white adipose tissue and large intestine and specific changes in the recipient's microbiota composition observed.
32516027	11	65	theme	ET	1877:1878	arg1	effect					1867:1872	the protective metabolic effect	1842:1872	the protective metabolic effect of ET on glucose metabolism	1842:1900	However, the protective metabolic effect of ET on glucose metabolism is not mediated through fecal factors.
32516027	7	66	dep	training	1263:1270	arg1	ET					1273:1274	ET	1273:1274	ET	1273:1274	The HFHS diet led to glucose intolerance and obesity in the donors, whereas exercise training (ET) restrained adiposity and improved glucose tolerance.
32516027	8	67	theme	body	1367:1370	arg1	composition					1372:1382	recipient body composition	1357:1382	recipient body composition	1357:1382	No donor group FMT altered recipient body composition.
32516027	0	68	theme	recipient	91:99	arg1	mice					101:104	recipient mice	91:104	recipient mice	91:104	Fecal microbiota transplantation from high caloric-fed donors alters glucose metabolism in recipient mice, independently of adiposity or exercise status.
32516027	5	69	dep	sedentary	893:901	arg1	NC-Sed					904:909	NC-Sed	904:909	NC-Sed	904:909	Mice were divided into sedentary (Sed) or ET groups (6 wk treadmill-based ET) while maintaining their diets, resulting in four donor groups: chow sedentary (NC-Sed) or ET (NC-ET) and HFHS sedentary (HFHS-Sed) or ET (HFHS-ET).
32516027	9	70	theme	glucose	1414:1420	arg1	levels					1422:1427	glucose levels	1414:1427	glucose levels	1414:1427	Despite unaltered adiposity, glucose levels were disrupted when challenged in mice receiving feces from HFHS-fed donors, irrespective of donor-ET status, with a decrease in insulin-stimulated glucose clearance into white adipose tissue and large intestine and specific changes in the recipient's microbiota composition observed.
32516027	7	71	from	obesity	1223:1229	arg1	donors					1238:1243	the donors	1234:1243	the donors	1234:1243	The HFHS diet led to glucose intolerance and obesity in the donors, whereas exercise training (ET) restrained adiposity and improved glucose tolerance.
32516027	7	72	theme	glucose	1199:1205	arg1	intolerance					1207:1217	glucose intolerance	1199:1217	glucose intolerance	1199:1217	The HFHS diet led to glucose intolerance and obesity in the donors, whereas exercise training (ET) restrained adiposity and improved glucose tolerance.
32516027	3	73	theme	protective	367:376	arg1	effects					388:394	the protective metabolic effects	363:394	the protective metabolic effects of exercise	363:406	We tested whether the protective metabolic effects of exercise are mediated through fecal components through assessment of body composition and metabolism in recipients of fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets.
32516027	3	74	theme	composition	473:483	arg1	assessment					454:463	assessment	454:463	assessment of body composition and metabolism in recipients of fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets	454:618	We tested whether the protective metabolic effects of exercise are mediated through fecal components through assessment of body composition and metabolism in recipients of fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets.
32516027	0	75	theme	microbiota	6:15	arg1	transplantation					17:31	Fecal microbiota transplantation	0:31	Fecal microbiota transplantation from high caloric-fed donors	0:60	Fecal microbiota transplantation from high caloric-fed donors alters glucose metabolism in recipient mice, independently of adiposity or exercise status.
32516027	7	76	theme	HFHS	1182:1185	arg1	diet					1187:1190	The HFHS diet	1178:1190	The HFHS diet	1178:1190	The HFHS diet led to glucose intolerance and obesity in the donors, whereas exercise training (ET) restrained adiposity and improved glucose tolerance.
32516027	3	77	theme	exercise	399:406	arg1	effects					388:394	the protective metabolic effects	363:394	the protective metabolic effects of exercise	363:406	We tested whether the protective metabolic effects of exercise are mediated through fecal components through assessment of body composition and metabolism in recipients of fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets.
32516027	5	78	theme	sedentary	770:778	arg1	groups					792:797	sedentary (Sed) or ET groups	770:797	sedentary (Sed) or ET groups (6 wk treadmill-based ET)	770:823	Mice were divided into sedentary (Sed) or ET groups (6 wk treadmill-based ET) while maintaining their diets, resulting in four donor groups: chow sedentary (NC-Sed) or ET (NC-ET) and HFHS sedentary (HFHS-Sed) or ET (HFHS-ET).
32516027	6	79	theme	recipient	982:990	arg1	mice					992:995	Chow-fed recipient mice	973:995	Chow-fed recipient mice	973:995	Chow-fed recipient mice were gavaged with feces from the respective donor groups weekly, creating four groups (NC-Sed-R, NC-ET-R, HFHS-Sed-R, HFHS-ET-R), and body composition and metabolism were assessed.
32516027	10	80	theme	glucose	1754:1760	arg1	metabolism					1762:1771	HFHS-induced disrupted glucose metabolism	1731:1771	HFHS-induced disrupted glucose metabolism	1731:1771	FMT can transmit HFHS-induced disrupted glucose metabolism to recipient mice independently of any change in adiposity.
32516027	3	81	theme	metabolism	489:498	arg1	assessment					454:463	assessment	454:463	assessment of body composition and metabolism in recipients of fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets	454:618	We tested whether the protective metabolic effects of exercise are mediated through fecal components through assessment of body composition and metabolism in recipients of fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets.
32516027	6	82	theme	respective	1030:1039	arg1	groups					1047:1052	the respective donor groups	1026:1052	the respective donor groups	1026:1052	Chow-fed recipient mice were gavaged with feces from the respective donor groups weekly, creating four groups (NC-Sed-R, NC-ET-R, HFHS-Sed-R, HFHS-ET-R), and body composition and metabolism were assessed.
32516027	3	83	theme	fecal	429:433	arg1	components					435:444	fecal components	429:444	fecal components	429:444	We tested whether the protective metabolic effects of exercise are mediated through fecal components through assessment of body composition and metabolism in recipients of fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets.
32516027	3	84	theme	transplantation	534:548	arg1	recipients					503:512	recipients	503:512	recipients of fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets	503:618	We tested whether the protective metabolic effects of exercise are mediated through fecal components through assessment of body composition and metabolism in recipients of fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets.
32516027	9	85	from	changes	1654:1660	arg1	composition					1692:1702	the recipient's microbiota composition	1665:1702	the recipient's microbiota composition	1665:1702	Despite unaltered adiposity, glucose levels were disrupted when challenged in mice receiving feces from HFHS-fed donors, irrespective of donor-ET status, with a decrease in insulin-stimulated glucose clearance into white adipose tissue and large intestine and specific changes in the recipient's microbiota composition observed.
32516027	9	85	from	changes	1654:1660	arg1	clearance					1585:1593	insulin-stimulated glucose clearance	1558:1593	insulin-stimulated glucose clearance into white adipose tissue and large intestine	1558:1639	Despite unaltered adiposity, glucose levels were disrupted when challenged in mice receiving feces from HFHS-fed donors, irrespective of donor-ET status, with a decrease in insulin-stimulated glucose clearance into white adipose tissue and large intestine and specific changes in the recipient's microbiota composition observed.
32516027	3	86	from	assessment	454:463	arg1	recipients					503:512	recipients	503:512	recipients of fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets	503:618	We tested whether the protective metabolic effects of exercise are mediated through fecal components through assessment of body composition and metabolism in recipients of fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets.
32516027	3	87	theme	fecal	517:521	arg1	FMT					551:553	FMT	551:553	FMT	551:553	We tested whether the protective metabolic effects of exercise are mediated through fecal components through assessment of body composition and metabolism in recipients of fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets.
32516027	3	87	theme	fecal	517:521	arg1	transplantation					534:548	fecal microbiota transplantation	517:548	fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets	517:618	We tested whether the protective metabolic effects of exercise are mediated through fecal components through assessment of body composition and metabolism in recipients of fecal microbiota transplantation (FMT) from exercise-trained (ET) mice fed normal or high-energy diets.
32516027	1	88	theme	metabolic	235:243	arg1	syndrome					245:252	metabolic syndrome	235:252	metabolic syndrome	235:252	Studies suggest the gut microbiota contributes to the development of obesity and metabolic syndrome.
34051252	0	0	theme	release	76:82	arg1	properties					84:93	structural, antimicrobial and controlled release properties	35:93	structural, antimicrobial and controlled release properties of fish gelatin-chitosan composite films	35:134	The effect of cross-linker type on structural, antimicrobial and controlled release properties of fish gelatin-chitosan composite films incorporated with ε-poly-l-lysine.
34051252	4	1	theme	G	536:536	arg1	addition					524:531	The addition	520:531	The addition of G to the biopolymer mixture	520:562	The addition of G to the biopolymer mixture significantly resulted in lower water vapor permeability, enhanced mechanical strength, lower moisture content, and water solubility.
34051252	9	2	theme	steady-state	1081:1092	arg1	properties					1117:1126	higher and steady-state in vitro antimicrobial properties	1070:1126	higher and steady-state in vitro antimicrobial properties against food spoilage bacteria	1070:1157	The composite films incorporated with ε-PLL revealed higher and steady-state in vitro antimicrobial properties against food spoilage bacteria.
34051252	4	3	theme	enhanced	622:629	arg1	strength					642:649	enhanced mechanical strength	622:649	enhanced mechanical strength	622:649	The addition of G to the biopolymer mixture significantly resulted in lower water vapor permeability, enhanced mechanical strength, lower moisture content, and water solubility.
34051252	0	4	theme	controlled	65:74	arg1	properties					84:93	structural, antimicrobial and controlled release properties	35:93	structural, antimicrobial and controlled release properties of fish gelatin-chitosan composite films	35:134	The effect of cross-linker type on structural, antimicrobial and controlled release properties of fish gelatin-chitosan composite films incorporated with ε-poly-l-lysine.
34051252	0	5	from	effect	4:9	arg1	properties					84:93	structural, antimicrobial and controlled release properties	35:93	structural, antimicrobial and controlled release properties of fish gelatin-chitosan composite films	35:134	The effect of cross-linker type on structural, antimicrobial and controlled release properties of fish gelatin-chitosan composite films incorporated with ε-poly-l-lysine.
34051252	8	6	theme	composite	1001:1009	arg1	film					1011:1014	the composite film	997:1014	the composite film	997:1014	C induced very small pores in the cross-section of the composite film.
34051252	7	7	theme	smooth	905:910	arg1	surfaces					922:929	smooth and rough surfaces	905:929	smooth and rough surfaces	905:929	The films cross-linked by G and C showed smooth and rough surfaces, respectively.
34051252	5	8	theme	composite	761:769	arg1	network					776:782	the composite film network	757:782	the composite film network	757:782	The FTIR spectra indicated the formation of imine bonds in the composite film network.
34051252	1	9	link	cross-linked	207:218	arg1	films					230:234	active antibacterial cross-linked composite films	186:234	active antibacterial cross-linked composite films	186:234	In this study, active antibacterial cross-linked composite films were prepared through incorporating ε-poly-l-lysine (ε-PLL) into Siberian sturgeon gelatin-chitosan mixture.
34051252	5	10	theme	film	771:774	arg1	network					776:782	the composite film network	757:782	the composite film network	757:782	The FTIR spectra indicated the formation of imine bonds in the composite film network.
34051252	5	11	theme	bonds	748:752	arg1	formation					729:737	the formation	725:737	the formation of imine bonds in the composite film network	725:782	The FTIR spectra indicated the formation of imine bonds in the composite film network.
34051252	6	12	theme	cross-linking	843:855	arg1	agent					857:861	the cross-linking agent	839:861	the cross-linking agent	839:861	The microstructure of composite films was affected by the cross-linking agent.
34051252	9	13	theme	antimicrobial	1103:1115	arg1	properties					1117:1126	higher and steady-state in vitro antimicrobial properties	1070:1126	higher and steady-state in vitro antimicrobial properties against food spoilage bacteria	1070:1157	The composite films incorporated with ε-PLL revealed higher and steady-state in vitro antimicrobial properties against food spoilage bacteria.
34051252	4	14	theme	mechanical	631:640	arg1	strength					642:649	enhanced mechanical strength	622:649	enhanced mechanical strength	622:649	The addition of G to the biopolymer mixture significantly resulted in lower water vapor permeability, enhanced mechanical strength, lower moisture content, and water solubility.
34051252	0	15	theme	gelatin-chitosan	103:118	arg1	films					130:134	fish gelatin-chitosan composite films	98:134	fish gelatin-chitosan composite films	98:134	The effect of cross-linker type on structural, antimicrobial and controlled release properties of fish gelatin-chitosan composite films incorporated with ε-poly-l-lysine.
34051252	11	16	theme	gelatin/chitosan	1352:1367	arg1	films					1379:1383	the ε-PLL-fortified fish gelatin/chitosan composite films	1327:1383	the ε-PLL-fortified fish gelatin/chitosan composite films	1327:1383	The results showed that the ε-PLL-fortified fish gelatin/chitosan composite films can be considered as a food-packaging material.
34051252	11	16	theme	gelatin/chitosan	1352:1367	arg1	material					1423:1430	a food-packaging material	1406:1430	a food-packaging material	1406:1430	The results showed that the ε-PLL-fortified fish gelatin/chitosan composite films can be considered as a food-packaging material.
34051252	8	17	theme	film	1011:1014	arg1	cross-section					980:992	the cross-section	976:992	the cross-section of the composite film	976:1014	C induced very small pores in the cross-section of the composite film.
34051252	0	18	theme	fish	98:101	arg1	films					130:134	fish gelatin-chitosan composite films	98:134	fish gelatin-chitosan composite films	98:134	The effect of cross-linker type on structural, antimicrobial and controlled release properties of fish gelatin-chitosan composite films incorporated with ε-poly-l-lysine.
34051252	12	19	theme	structural	1476:1485	arg1	properties					1505:1514	the structural and antimicrobial properties	1472:1514	the structural and antimicrobial properties of this composite film	1472:1537	G and C, as cross-linkers, can improve the structural and antimicrobial properties of this composite film.
34051252	11	20	theme	composite	1369:1377	arg1	films					1379:1383	the ε-PLL-fortified fish gelatin/chitosan composite films	1327:1383	the ε-PLL-fortified fish gelatin/chitosan composite films	1327:1383	The results showed that the ε-PLL-fortified fish gelatin/chitosan composite films can be considered as a food-packaging material.
34051252	11	20	theme	composite	1369:1377	arg1	material					1423:1430	a food-packaging material	1406:1430	a food-packaging material	1406:1430	The results showed that the ε-PLL-fortified fish gelatin/chitosan composite films can be considered as a food-packaging material.
34051252	9	21	dep	in	1094:1095	arg1	vitro					1097:1101	vitro	1097:1101	vitro	1097:1101	The composite films incorporated with ε-PLL revealed higher and steady-state in vitro antimicrobial properties against food spoilage bacteria.
34051252	3	22	theme	morphological	471:483	arg1	antimicrobial					426:438	antimicrobial	426:438	antimicrobial	426:438	The antimicrobial, ε-PLL release, mechanical and morphological properties were then investigated.
34051252	3	22	theme	morphological	471:483	arg1	properties					485:494	mechanical and morphological properties	456:494	mechanical and morphological properties	456:494	The antimicrobial, ε-PLL release, mechanical and morphological properties were then investigated.
34051252	10	23	theme	higher	1162:1167	arg1	release					1169:1175	A higher release	1160:1175	A higher release of ε-PLL	1160:1184	A higher release of ε-PLL and hence higher antibacterial activity was measured in the matrices cross-linked by C than those cross-linked by G.
34051252	11	24	theme	ε-PLL-fortified	1331:1345	arg1	films					1379:1383	the ε-PLL-fortified fish gelatin/chitosan composite films	1327:1383	the ε-PLL-fortified fish gelatin/chitosan composite films	1327:1383	The results showed that the ε-PLL-fortified fish gelatin/chitosan composite films can be considered as a food-packaging material.
34051252	11	24	theme	ε-PLL-fortified	1331:1345	arg1	material					1423:1430	a food-packaging material	1406:1430	a food-packaging material	1406:1430	The results showed that the ε-PLL-fortified fish gelatin/chitosan composite films can be considered as a food-packaging material.
34051252	4	25	theme	water	596:600	arg1	permeability					608:619	lower water vapor permeability	590:619	lower water vapor permeability	590:619	The addition of G to the biopolymer mixture significantly resulted in lower water vapor permeability, enhanced mechanical strength, lower moisture content, and water solubility.
34051252	9	26	theme	food	1136:1139	arg1	spoilage					1141:1148	food spoilage	1136:1148	food spoilage bacteria	1136:1157	The composite films incorporated with ε-PLL revealed higher and steady-state in vitro antimicrobial properties against food spoilage bacteria.
34051252	1	27	theme	active	186:191	arg1	films					230:234	active antibacterial cross-linked composite films	186:234	active antibacterial cross-linked composite films	186:234	In this study, active antibacterial cross-linked composite films were prepared through incorporating ε-poly-l-lysine (ε-PLL) into Siberian sturgeon gelatin-chitosan mixture.
34051252	4	28	theme	biopolymer	545:554	arg1	mixture					556:562	the biopolymer mixture	541:562	the biopolymer mixture	541:562	The addition of G to the biopolymer mixture significantly resulted in lower water vapor permeability, enhanced mechanical strength, lower moisture content, and water solubility.
34051252	1	29	theme	Siberian	301:308	arg1	mixture					336:342	Siberian sturgeon gelatin-chitosan mixture	301:342	Siberian sturgeon gelatin-chitosan mixture	301:342	In this study, active antibacterial cross-linked composite films were prepared through incorporating ε-poly-l-lysine (ε-PLL) into Siberian sturgeon gelatin-chitosan mixture.
34051252	11	30	theme	fish	1347:1350	arg1	films					1379:1383	the ε-PLL-fortified fish gelatin/chitosan composite films	1327:1383	the ε-PLL-fortified fish gelatin/chitosan composite films	1327:1383	The results showed that the ε-PLL-fortified fish gelatin/chitosan composite films can be considered as a food-packaging material.
34051252	11	30	theme	fish	1347:1350	arg1	material					1423:1430	a food-packaging material	1406:1430	a food-packaging material	1406:1430	The results showed that the ε-PLL-fortified fish gelatin/chitosan composite films can be considered as a food-packaging material.
34051252	9	31	theme	spoilage	1141:1148	arg1	bacteria					1150:1157	food spoilage bacteria	1136:1157	food spoilage bacteria	1136:1157	The composite films incorporated with ε-PLL revealed higher and steady-state in vitro antimicrobial properties against food spoilage bacteria.
34051252	1	32	theme	antibacterial	193:205	arg1	films					230:234	active antibacterial cross-linked composite films	186:234	active antibacterial cross-linked composite films	186:234	In this study, active antibacterial cross-linked composite films were prepared through incorporating ε-poly-l-lysine (ε-PLL) into Siberian sturgeon gelatin-chitosan mixture.
34051252	0	33	theme	cross-linker	14:25	arg1	type					27:30	cross-linker type	14:30	cross-linker type	14:30	The effect of cross-linker type on structural, antimicrobial and controlled release properties of fish gelatin-chitosan composite films incorporated with ε-poly-l-lysine.
34051252	1	34	theme	sturgeon	310:317	arg1	mixture					336:342	Siberian sturgeon gelatin-chitosan mixture	301:342	Siberian sturgeon gelatin-chitosan mixture	301:342	In this study, active antibacterial cross-linked composite films were prepared through incorporating ε-poly-l-lysine (ε-PLL) into Siberian sturgeon gelatin-chitosan mixture.
34051252	0	35	theme	films	130:134	arg1	properties					84:93	structural, antimicrobial and controlled release properties	35:93	structural, antimicrobial and controlled release properties of fish gelatin-chitosan composite films	35:134	The effect of cross-linker type on structural, antimicrobial and controlled release properties of fish gelatin-chitosan composite films incorporated with ε-poly-l-lysine.
34051252	6	36	theme	films	817:821	arg1	microstructure					789:802	The microstructure	785:802	The microstructure of composite films	785:821	The microstructure of composite films was affected by the cross-linking agent.
34051252	1	37	theme	cross-linked	207:218	arg1	films					230:234	active antibacterial cross-linked composite films	186:234	active antibacterial cross-linked composite films	186:234	In this study, active antibacterial cross-linked composite films were prepared through incorporating ε-poly-l-lysine (ε-PLL) into Siberian sturgeon gelatin-chitosan mixture.
34051252	1	38	theme	gelatin-chitosan	319:334	arg1	mixture					336:342	Siberian sturgeon gelatin-chitosan mixture	301:342	Siberian sturgeon gelatin-chitosan mixture	301:342	In this study, active antibacterial cross-linked composite films were prepared through incorporating ε-poly-l-lysine (ε-PLL) into Siberian sturgeon gelatin-chitosan mixture.
34051252	0	39	theme	composite	120:128	arg1	films					130:134	fish gelatin-chitosan composite films	98:134	fish gelatin-chitosan composite films	98:134	The effect of cross-linker type on structural, antimicrobial and controlled release properties of fish gelatin-chitosan composite films incorporated with ε-poly-l-lysine.
34051252	1	40	theme	composite	220:228	arg1	films					230:234	active antibacterial cross-linked composite films	186:234	active antibacterial cross-linked composite films	186:234	In this study, active antibacterial cross-linked composite films were prepared through incorporating ε-poly-l-lysine (ε-PLL) into Siberian sturgeon gelatin-chitosan mixture.
34051252	3	41	theme	ε-PLL	441:445	arg1	antimicrobial					426:438	antimicrobial	426:438	antimicrobial	426:438	The antimicrobial, ε-PLL release, mechanical and morphological properties were then investigated.
34051252	3	41	theme	ε-PLL	441:445	arg1	release					447:453	ε-PLL release	441:453	ε-PLL release	441:453	The antimicrobial, ε-PLL release, mechanical and morphological properties were then investigated.
34051252	4	42	theme	vapor	602:606	arg1	permeability					608:619	lower water vapor permeability	590:619	lower water vapor permeability	590:619	The addition of G to the biopolymer mixture significantly resulted in lower water vapor permeability, enhanced mechanical strength, lower moisture content, and water solubility.
34051252	9	43	theme	in	1094:1095	arg1	properties					1117:1126	higher and steady-state in vitro antimicrobial properties	1070:1126	higher and steady-state in vitro antimicrobial properties against food spoilage bacteria	1070:1157	The composite films incorporated with ε-PLL revealed higher and steady-state in vitro antimicrobial properties against food spoilage bacteria.
34051252	3	44	theme	mechanical	456:465	arg1	antimicrobial					426:438	antimicrobial	426:438	antimicrobial	426:438	The antimicrobial, ε-PLL release, mechanical and morphological properties were then investigated.
34051252	3	44	theme	mechanical	456:465	arg1	properties					485:494	mechanical and morphological properties	456:494	mechanical and morphological properties	456:494	The antimicrobial, ε-PLL release, mechanical and morphological properties were then investigated.
34051252	0	45	theme	type	27:30	arg1	effect					4:9	The effect	0:9	The effect of cross-linker type on structural, antimicrobial and controlled release properties of fish gelatin-chitosan composite films	0:134	The effect of cross-linker type on structural, antimicrobial and controlled release properties of fish gelatin-chitosan composite films incorporated with ε-poly-l-lysine.
34051252	10	46	theme	antibacterial	1203:1215	arg1	activity					1217:1224	hence higher antibacterial activity	1190:1224	hence higher antibacterial activity	1190:1224	A higher release of ε-PLL and hence higher antibacterial activity was measured in the matrices cross-linked by C than those cross-linked by G.
34051252	9	47	theme	composite	1021:1029	arg1	films					1031:1035	The composite films	1017:1035	The composite films incorporated with ε-PLL	1017:1059	The composite films incorporated with ε-PLL revealed higher and steady-state in vitro antimicrobial properties against food spoilage bacteria.
34051252	6	48	theme	composite	807:815	arg1	films					817:821	composite films	807:821	composite films	807:821	The microstructure of composite films was affected by the cross-linking agent.
34051252	4	49	theme	water	680:684	arg1	solubility					686:695	water solubility	680:695	water solubility	680:695	The addition of G to the biopolymer mixture significantly resulted in lower water vapor permeability, enhanced mechanical strength, lower moisture content, and water solubility.
34051252	11	50	theme	food-packaging	1408:1421	arg1	films					1379:1383	the ε-PLL-fortified fish gelatin/chitosan composite films	1327:1383	the ε-PLL-fortified fish gelatin/chitosan composite films	1327:1383	The results showed that the ε-PLL-fortified fish gelatin/chitosan composite films can be considered as a food-packaging material.
34051252	11	50	theme	food-packaging	1408:1421	arg1	material					1423:1430	a food-packaging material	1406:1430	a food-packaging material	1406:1430	The results showed that the ε-PLL-fortified fish gelatin/chitosan composite films can be considered as a food-packaging material.
34051252	10	51	theme	higher	1196:1201	arg1	activity					1217:1224	hence higher antibacterial activity	1190:1224	hence higher antibacterial activity	1190:1224	A higher release of ε-PLL and hence higher antibacterial activity was measured in the matrices cross-linked by C than those cross-linked by G.
34051252	4	52	theme	lower	590:594	arg1	permeability					608:619	lower water vapor permeability	590:619	lower water vapor permeability	590:619	The addition of G to the biopolymer mixture significantly resulted in lower water vapor permeability, enhanced mechanical strength, lower moisture content, and water solubility.
34051252	0	53	theme	structural	35:44	arg1	properties					84:93	structural, antimicrobial and controlled release properties	35:93	structural, antimicrobial and controlled release properties of fish gelatin-chitosan composite films	35:134	The effect of cross-linker type on structural, antimicrobial and controlled release properties of fish gelatin-chitosan composite films incorporated with ε-poly-l-lysine.
34051252	5	54	theme	imine	742:746	arg1	bonds					748:752	imine bonds	742:752	imine bonds	742:752	The FTIR spectra indicated the formation of imine bonds in the composite film network.
34051252	12	55	theme	composite	1524:1532	arg1	film					1534:1537	this composite film	1519:1537	this composite film	1519:1537	G and C, as cross-linkers, can improve the structural and antimicrobial properties of this composite film.
34051252	5	56	theme	FTIR	702:705	arg1	spectra					707:713	The FTIR spectra	698:713	The FTIR spectra	698:713	The FTIR spectra indicated the formation of imine bonds in the composite film network.
34051252	10	57	theme	ε-PLL	1180:1184	arg1	activity					1217:1224	hence higher antibacterial activity	1190:1224	hence higher antibacterial activity	1190:1224	A higher release of ε-PLL and hence higher antibacterial activity was measured in the matrices cross-linked by C than those cross-linked by G.
34051252	10	57	theme	ε-PLL	1180:1184	arg1	release					1169:1175	A higher release	1160:1175	A higher release of ε-PLL	1160:1184	A higher release of ε-PLL and hence higher antibacterial activity was measured in the matrices cross-linked by C than those cross-linked by G.
34051252	8	58	theme	small	961:965	arg1	pores					967:971	very small pores	956:971	very small pores	956:971	C induced very small pores in the cross-section of the composite film.
34051252	4	59	theme	lower	652:656	arg1	content					667:673	lower moisture content	652:673	lower moisture content	652:673	The addition of G to the biopolymer mixture significantly resulted in lower water vapor permeability, enhanced mechanical strength, lower moisture content, and water solubility.
34051252	12	60	theme	film	1534:1537	arg1	properties					1505:1514	the structural and antimicrobial properties	1472:1514	the structural and antimicrobial properties of this composite film	1472:1537	G and C, as cross-linkers, can improve the structural and antimicrobial properties of this composite film.
34051252	9	61	theme	higher	1070:1075	arg1	properties					1117:1126	higher and steady-state in vitro antimicrobial properties	1070:1126	higher and steady-state in vitro antimicrobial properties against food spoilage bacteria	1070:1157	The composite films incorporated with ε-PLL revealed higher and steady-state in vitro antimicrobial properties against food spoilage bacteria.
34051252	0	62	theme	antimicrobial	47:59	arg1	properties					84:93	structural, antimicrobial and controlled release properties	35:93	structural, antimicrobial and controlled release properties of fish gelatin-chitosan composite films	35:134	The effect of cross-linker type on structural, antimicrobial and controlled release properties of fish gelatin-chitosan composite films incorporated with ε-poly-l-lysine.
34051252	12	63	theme	antimicrobial	1491:1503	arg1	properties					1505:1514	the structural and antimicrobial properties	1472:1514	the structural and antimicrobial properties of this composite film	1472:1537	G and C, as cross-linkers, can improve the structural and antimicrobial properties of this composite film.
34051252	5	64	from	formation	729:737	arg1	network					776:782	the composite film network	757:782	the composite film network	757:782	The FTIR spectra indicated the formation of imine bonds in the composite film network.
34051252	7	65	theme	rough	916:920	arg1	surfaces					922:929	smooth and rough surfaces	905:929	smooth and rough surfaces	905:929	The films cross-linked by G and C showed smooth and rough surfaces, respectively.
34051252	4	66	theme	moisture	658:665	arg1	content					667:673	lower moisture content	652:673	lower moisture content	652:673	The addition of G to the biopolymer mixture significantly resulted in lower water vapor permeability, enhanced mechanical strength, lower moisture content, and water solubility.
33149572	4	0	theme	antimicrobial	932:944	arg1	potential					946:954	antimicrobial potential	932:954	antimicrobial potential	932:954	The surface characterization, in vitro degradation under various pH values, antimicrobial potential, cytocompatibility and in vivo therapeutic efficacy in a bacteria-infected full-thickness skin defect model were systematically evaluated.
33149572	3	1	theme	acid	751:754	arg1	C/H					767:769	C/H	767:769	C/H	767:769	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	3	1	theme	acid	751:754	arg1	dressings					817:825	GO-incorporated chitosan/hyaluronic acid dressings	776:825	GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO)	776:834	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	3	1	theme	acid	751:754	arg1	dressings					756:764	chitosan/hyaluronic acid dressings	731:764	chitosan/hyaluronic acid dressings (C/H)	731:770	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	3	1	theme	acid	751:754	arg1	controls					846:853	controls	846:853	controls	846:853	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	7	2	theme	improved	1616:1623	arg1	angiogenesis					1625:1636	improved angiogenesis	1616:1636	improved angiogenesis in granulation tissues	1616:1659	Moreover, remarkably accelerated wound healing was found in the C/H/GO/Cu group, with controlled inflammatory infiltration and improved angiogenesis in granulation tissues.
33149572	9	3	theme	healing	2037:2043	arg1	process					2045:2051	a significantly accelerated healing process	2009:2051	a significantly accelerated healing process of bacteria-infected wounds	2009:2079	CONCLUSION Collectively, GO/Cu-incorporated chitosan/hyaluronic acid dressings suggested a synergistic antimicrobial efficacy and acceptable biocompatibility both in vitro and in vivo, as well as a significantly accelerated healing process of bacteria-infected wounds.
33149572	3	4	theme	GO-incorporated	776:790	arg1	dressings					756:764	chitosan/hyaluronic acid dressings	731:764	chitosan/hyaluronic acid dressings (C/H)	731:770	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	3	4	theme	GO-incorporated	776:790	arg1	C/H/GO					828:833	C/H/GO	828:833	C/H/GO	828:833	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	3	4	theme	GO-incorporated	776:790	arg1	dressings					817:825	GO-incorporated chitosan/hyaluronic acid dressings	776:825	GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO)	776:834	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	3	4	theme	GO-incorporated	776:790	arg1	controls					846:853	controls	846:853	controls	846:853	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	2	5	theme	wound	317:321	arg1	dressing					323:330	a novel and multifunctional wound dressing	289:330	a novel and multifunctional wound dressing	289:330	This study aimed to manufacture a novel and multifunctional wound dressing by combining graphene oxide/copper nanocomposites (GO/Cu) with chitosan/hyaluronic acid, providing significant opportunities for the therapy of wound repair in wounds with a high risk of bacterial infection.
33149572	10	6	theme	potential	2148:2156	arg1	composite					2118:2126	the multifunctional C/H/GO/Cu composite	2088:2126	the multifunctional C/H/GO/Cu composite	2088:2126	Thus, the multifunctional C/H/GO/Cu composite is expected to be a potential alternative for wound dressings, especially for the management of intractable wounds caused by bacterial infection.
33149572	10	6	theme	potential	2148:2156	arg1	alternative					2158:2168	a potential alternative	2146:2168	a potential alternative for wound dressings, especially for the management of intractable wounds caused by bacterial infection	2146:2271	Thus, the multifunctional C/H/GO/Cu composite is expected to be a potential alternative for wound dressings, especially for the management of intractable wounds caused by bacterial infection.
33149572	4	7	theme	various	913:919	arg1	values					924:929	various pH values	913:929	various pH values	913:929	The surface characterization, in vitro degradation under various pH values, antimicrobial potential, cytocompatibility and in vivo therapeutic efficacy in a bacteria-infected full-thickness skin defect model were systematically evaluated.
33149572	8	8	from	structures	1722:1731	arg1	any					1789:1791	any	1789:1791	any	1789:1791	In addition, no pathological damage was noted in the tissue structures of the tested organs (heart, lung, liver and kidney) in any of the four groups.
33149572	3	9	theme	chitosan/hyaluronic	579:597	arg1	C/H/GO/Cu					615:623	C/H/GO/Cu	615:623	C/H/GO/Cu	615:623	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	3	9	theme	chitosan/hyaluronic	579:597	arg1	dressings					604:612	GO/Cu-decorated chitosan/hyaluronic acid dressings	563:612	GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu)	563:624	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	3	9	theme	chitosan/hyaluronic	579:597	arg1	METHODS					540:546	METHODS	540:546	METHODS In this study	540:560	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	7	10	theme	controlled	1575:1584	arg1	infiltration					1599:1610	controlled inflammatory infiltration	1575:1610	controlled inflammatory infiltration	1575:1610	Moreover, remarkably accelerated wound healing was found in the C/H/GO/Cu group, with controlled inflammatory infiltration and improved angiogenesis in granulation tissues.
33149572	9	11	theme	wounds	2074:2079	arg1	process					2045:2051	a significantly accelerated healing process	2009:2051	a significantly accelerated healing process of bacteria-infected wounds	2009:2079	CONCLUSION Collectively, GO/Cu-incorporated chitosan/hyaluronic acid dressings suggested a synergistic antimicrobial efficacy and acceptable biocompatibility both in vitro and in vivo, as well as a significantly accelerated healing process of bacteria-infected wounds.
33149572	9	11	theme	wounds	2074:2079	arg1	efficacy					1930:1937	a synergistic antimicrobial efficacy	1902:1937	a synergistic antimicrobial efficacy	1902:1937	CONCLUSION Collectively, GO/Cu-incorporated chitosan/hyaluronic acid dressings suggested a synergistic antimicrobial efficacy and acceptable biocompatibility both in vitro and in vivo, as well as a significantly accelerated healing process of bacteria-infected wounds.
33149572	9	11	theme	wounds	2074:2079	arg1	biocompatibility					1954:1969	acceptable biocompatibility	1943:1969	acceptable biocompatibility	1943:1969	CONCLUSION Collectively, GO/Cu-incorporated chitosan/hyaluronic acid dressings suggested a synergistic antimicrobial efficacy and acceptable biocompatibility both in vitro and in vivo, as well as a significantly accelerated healing process of bacteria-infected wounds.
33149572	2	12	theme	graphene	345:352	arg1	GO/Cu					383:387	GO/Cu	383:387	GO/Cu	383:387	This study aimed to manufacture a novel and multifunctional wound dressing by combining graphene oxide/copper nanocomposites (GO/Cu) with chitosan/hyaluronic acid, providing significant opportunities for the therapy of wound repair in wounds with a high risk of bacterial infection.
33149572	2	12	theme	graphene	345:352	arg1	nanocomposites					367:380	graphene oxide/copper nanocomposites	345:380	graphene oxide/copper nanocomposites (GO/Cu)	345:388	This study aimed to manufacture a novel and multifunctional wound dressing by combining graphene oxide/copper nanocomposites (GO/Cu) with chitosan/hyaluronic acid, providing significant opportunities for the therapy of wound repair in wounds with a high risk of bacterial infection.
33149572	1	13	theme	clinical	218:225	arg1	challenge					227:235	a clinical challenge	216:235	a clinical challenge in plastic surgery	216:254	PURPOSE Wound healing, especially of infected wounds, remains a clinical challenge in plastic surgery.
33149572	3	14	theme	acid	812:815	arg1	dressings					756:764	chitosan/hyaluronic acid dressings	731:764	chitosan/hyaluronic acid dressings (C/H)	731:770	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	3	14	theme	acid	812:815	arg1	C/H/GO					828:833	C/H/GO	828:833	C/H/GO	828:833	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	3	14	theme	acid	812:815	arg1	dressings					817:825	GO-incorporated chitosan/hyaluronic acid dressings	776:825	GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO)	776:834	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	3	14	theme	acid	812:815	arg1	controls					846:853	controls	846:853	controls	846:853	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	6	15	with	cytocompatibility	1434:1450	arg1	NIH/3T3-L1					1476:1485	NIH/3T3-L1	1476:1485	NIH/3T3-L1	1476:1485	All three dressings showed satisfactory cytocompatibility with mouse fibroblasts (NIH/3T3-L1).
33149572	6	15	with	cytocompatibility	1434:1450	arg1	fibroblasts					1463:1473	mouse fibroblasts	1457:1473	mouse fibroblasts (NIH/3T3-L1)	1457:1486	All three dressings showed satisfactory cytocompatibility with mouse fibroblasts (NIH/3T3-L1).
33149572	7	16	theme	accelerated	1510:1520	arg1	healing					1528:1534	remarkably accelerated wound healing	1499:1534	remarkably accelerated wound healing	1499:1534	Moreover, remarkably accelerated wound healing was found in the C/H/GO/Cu group, with controlled inflammatory infiltration and improved angiogenesis in granulation tissues.
33149572	4	17	from	efficacy	999:1006	arg1	model					1058:1062	a bacteria-infected full-thickness skin defect model	1011:1062	a bacteria-infected full-thickness skin defect model	1011:1062	The surface characterization, in vitro degradation under various pH values, antimicrobial potential, cytocompatibility and in vivo therapeutic efficacy in a bacteria-infected full-thickness skin defect model were systematically evaluated.
33149572	3	18	theme	trimetaphosphate	653:668	arg1	crosslinking					677:688	sodium trimetaphosphate (STMP) crosslinking	646:688	sodium trimetaphosphate (STMP) crosslinking	646:688	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	4	19	from	degradation	895:905	arg1	model					1058:1062	a bacteria-infected full-thickness skin defect model	1011:1062	a bacteria-infected full-thickness skin defect model	1011:1062	The surface characterization, in vitro degradation under various pH values, antimicrobial potential, cytocompatibility and in vivo therapeutic efficacy in a bacteria-infected full-thickness skin defect model were systematically evaluated.
33149572	7	20	from	angiogenesis	1625:1636	arg1	tissues					1653:1659	granulation tissues	1641:1659	granulation tissues	1641:1659	Moreover, remarkably accelerated wound healing was found in the C/H/GO/Cu group, with controlled inflammatory infiltration and improved angiogenesis in granulation tissues.
33149572	5	21	theme	significant	1280:1290	arg1	activities					1315:1324	significant in vitro antimicrobial activities	1280:1324	significant in vitro antimicrobial activities against the two tested bacterial strains	1280:1365	RESULTS Our experimental results indicated that the acidic environment facilitated the release of copper (CuNPs and Cu2+) from the dressings, and prepared C/H/GO/Cu dressings exhibited significant in vitro antimicrobial activities against the two tested bacterial strains (ATCC35984 and ATCC25923).
33149572	6	22	theme	satisfactory	1421:1432	arg1	cytocompatibility					1434:1450	satisfactory cytocompatibility	1421:1450	satisfactory cytocompatibility with mouse fibroblasts (NIH/3T3-L1)	1421:1486	All three dressings showed satisfactory cytocompatibility with mouse fibroblasts (NIH/3T3-L1).
33149572	5	23	theme	acidic	1147:1152	arg1	environment					1154:1164	the acidic environment	1143:1164	the acidic environment	1143:1164	RESULTS Our experimental results indicated that the acidic environment facilitated the release of copper (CuNPs and Cu2+) from the dressings, and prepared C/H/GO/Cu dressings exhibited significant in vitro antimicrobial activities against the two tested bacterial strains (ATCC35984 and ATCC25923).
33149572	1	24	from	challenge	227:235	arg1	surgery					248:254	plastic surgery	240:254	plastic surgery	240:254	PURPOSE Wound healing, especially of infected wounds, remains a clinical challenge in plastic surgery.
33149572	1	25	theme	Wound	162:166	arg1	healing					168:174	PURPOSE Wound healing	154:174	PURPOSE Wound healing	154:174	PURPOSE Wound healing, especially of infected wounds, remains a clinical challenge in plastic surgery.
33149572	9	26	theme	GO/Cu-incorporated	1838:1855	arg1	dressings					1882:1890	GO/Cu-incorporated chitosan/hyaluronic acid dressings	1838:1890	GO/Cu-incorporated chitosan/hyaluronic acid dressings	1838:1890	CONCLUSION Collectively, GO/Cu-incorporated chitosan/hyaluronic acid dressings suggested a synergistic antimicrobial efficacy and acceptable biocompatibility both in vitro and in vivo, as well as a significantly accelerated healing process of bacteria-infected wounds.
33149572	10	27	theme	wound	2174:2178	arg1	dressings					2180:2188	wound dressings	2174:2188	wound dressings	2174:2188	Thus, the multifunctional C/H/GO/Cu composite is expected to be a potential alternative for wound dressings, especially for the management of intractable wounds caused by bacterial infection.
33149572	4	28	from	characterization	868:883	arg1	model					1058:1062	a bacteria-infected full-thickness skin defect model	1011:1062	a bacteria-infected full-thickness skin defect model	1011:1062	The surface characterization, in vitro degradation under various pH values, antimicrobial potential, cytocompatibility and in vivo therapeutic efficacy in a bacteria-infected full-thickness skin defect model were systematically evaluated.
33149572	9	29	theme	acid	1877:1880	arg1	dressings					1882:1890	GO/Cu-incorporated chitosan/hyaluronic acid dressings	1838:1890	GO/Cu-incorporated chitosan/hyaluronic acid dressings	1838:1890	CONCLUSION Collectively, GO/Cu-incorporated chitosan/hyaluronic acid dressings suggested a synergistic antimicrobial efficacy and acceptable biocompatibility both in vitro and in vivo, as well as a significantly accelerated healing process of bacteria-infected wounds.
33149572	9	30	dep	CONCLUSION	1813:1822	arg1	suggested					1892:1900	suggested	1892:1900	suggested a synergistic antimicrobial efficacy and acceptable biocompatibility both in vitro and in vivo, as well as a significantly accelerated healing process of bacteria-infected wounds	1892:2079	CONCLUSION Collectively, GO/Cu-incorporated chitosan/hyaluronic acid dressings suggested a synergistic antimicrobial efficacy and acceptable biocompatibility both in vitro and in vivo, as well as a significantly accelerated healing process of bacteria-infected wounds.
33149572	4	31	dep	in	979:980	arg1	vivo					982:985	vivo	982:985	vivo	982:985	The surface characterization, in vitro degradation under various pH values, antimicrobial potential, cytocompatibility and in vivo therapeutic efficacy in a bacteria-infected full-thickness skin defect model were systematically evaluated.
33149572	3	32	theme	STMP	671:674	arg1	crosslinking					677:688	sodium trimetaphosphate (STMP) crosslinking	646:688	sodium trimetaphosphate (STMP) crosslinking	646:688	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	2	33	theme	infection	529:537	arg1	risk					511:514	a high risk	504:514	a high risk of bacterial infection	504:537	This study aimed to manufacture a novel and multifunctional wound dressing by combining graphene oxide/copper nanocomposites (GO/Cu) with chitosan/hyaluronic acid, providing significant opportunities for the therapy of wound repair in wounds with a high risk of bacterial infection.
33149572	9	34	theme	chitosan/hyaluronic	1857:1875	arg1	dressings					1882:1890	GO/Cu-incorporated chitosan/hyaluronic acid dressings	1838:1890	GO/Cu-incorporated chitosan/hyaluronic acid dressings	1838:1890	CONCLUSION Collectively, GO/Cu-incorporated chitosan/hyaluronic acid dressings suggested a synergistic antimicrobial efficacy and acceptable biocompatibility both in vitro and in vivo, as well as a significantly accelerated healing process of bacteria-infected wounds.
33149572	1	35	theme	wounds	200:205	arg1	healing					168:174	PURPOSE Wound healing	154:174	PURPOSE Wound healing	154:174	PURPOSE Wound healing, especially of infected wounds, remains a clinical challenge in plastic surgery.
33149572	4	36	theme	full-thickness	1031:1044	arg1	model					1058:1062	a bacteria-infected full-thickness skin defect model	1011:1062	a bacteria-infected full-thickness skin defect model	1011:1062	The surface characterization, in vitro degradation under various pH values, antimicrobial potential, cytocompatibility and in vivo therapeutic efficacy in a bacteria-infected full-thickness skin defect model were systematically evaluated.
33149572	0	37	theme	Full-Thickness	125:138	arg1	Defects					145:151	Infected Full-Thickness Skin Defects	116:151	Infected Full-Thickness Skin Defects	116:151	Graphene Oxide/Copper Nanoderivatives-Modified Chitosan/Hyaluronic Acid Dressings for Facilitating Wound Healing in Infected Full-Thickness Skin Defects.
33149572	4	38	theme	defect	1051:1056	arg1	model					1058:1062	a bacteria-infected full-thickness skin defect model	1011:1062	a bacteria-infected full-thickness skin defect model	1011:1062	The surface characterization, in vitro degradation under various pH values, antimicrobial potential, cytocompatibility and in vivo therapeutic efficacy in a bacteria-infected full-thickness skin defect model were systematically evaluated.
33149572	9	39	theme	acceptable	1943:1952	arg1	biocompatibility					1954:1969	acceptable biocompatibility	1943:1969	acceptable biocompatibility	1943:1969	CONCLUSION Collectively, GO/Cu-incorporated chitosan/hyaluronic acid dressings suggested a synergistic antimicrobial efficacy and acceptable biocompatibility both in vitro and in vivo, as well as a significantly accelerated healing process of bacteria-infected wounds.
33149572	2	40	theme	significant	431:441	arg1	opportunities					443:455	significant opportunities	431:455	significant opportunities	431:455	This study aimed to manufacture a novel and multifunctional wound dressing by combining graphene oxide/copper nanocomposites (GO/Cu) with chitosan/hyaluronic acid, providing significant opportunities for the therapy of wound repair in wounds with a high risk of bacterial infection.
33149572	0	41	theme	Graphene	0:7	arg1	Dressings					72:80	Graphene Oxide/Copper Nanoderivatives-Modified Chitosan/Hyaluronic Acid Dressings	0:80	Graphene Oxide/Copper Nanoderivatives-Modified Chitosan/Hyaluronic Acid Dressings for Facilitating Wound Healing in Infected Full-Thickness Skin Defects.	0:152	Graphene Oxide/Copper Nanoderivatives-Modified Chitosan/Hyaluronic Acid Dressings for Facilitating Wound Healing in Infected Full-Thickness Skin Defects.
33149572	2	42	theme	wound	476:480	arg1	repair					482:487	wound repair	476:487	wound repair	476:487	This study aimed to manufacture a novel and multifunctional wound dressing by combining graphene oxide/copper nanocomposites (GO/Cu) with chitosan/hyaluronic acid, providing significant opportunities for the therapy of wound repair in wounds with a high risk of bacterial infection.
33149572	0	43	theme	Nanoderivatives-Modified	22:45	arg1	Dressings					72:80	Graphene Oxide/Copper Nanoderivatives-Modified Chitosan/Hyaluronic Acid Dressings	0:80	Graphene Oxide/Copper Nanoderivatives-Modified Chitosan/Hyaluronic Acid Dressings for Facilitating Wound Healing in Infected Full-Thickness Skin Defects.	0:152	Graphene Oxide/Copper Nanoderivatives-Modified Chitosan/Hyaluronic Acid Dressings for Facilitating Wound Healing in Infected Full-Thickness Skin Defects.
33149572	5	44	theme	in	1292:1293	arg1	activities					1315:1324	significant in vitro antimicrobial activities	1280:1324	significant in vitro antimicrobial activities against the two tested bacterial strains	1280:1365	RESULTS Our experimental results indicated that the acidic environment facilitated the release of copper (CuNPs and Cu2+) from the dressings, and prepared C/H/GO/Cu dressings exhibited significant in vitro antimicrobial activities against the two tested bacterial strains (ATCC35984 and ATCC25923).
33149572	3	45	theme	GO/Cu-decorated	563:577	arg1	C/H/GO/Cu					615:623	C/H/GO/Cu	615:623	C/H/GO/Cu	615:623	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	3	45	theme	GO/Cu-decorated	563:577	arg1	dressings					604:612	GO/Cu-decorated chitosan/hyaluronic acid dressings	563:612	GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu)	563:624	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	3	45	theme	GO/Cu-decorated	563:577	arg1	METHODS					540:546	METHODS	540:546	METHODS In this study	540:560	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	3	46	theme	freeze-drying	705:717	arg1	method					719:724	the vacuum freeze-drying method	694:724	the vacuum freeze-drying method	694:724	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	4	47	theme	in	886:887	arg1	degradation					895:905	in vitro degradation	886:905	in vitro degradation under various pH values	886:929	The surface characterization, in vitro degradation under various pH values, antimicrobial potential, cytocompatibility and in vivo therapeutic efficacy in a bacteria-infected full-thickness skin defect model were systematically evaluated.
33149572	0	48	theme	Acid	67:70	arg1	Dressings					72:80	Graphene Oxide/Copper Nanoderivatives-Modified Chitosan/Hyaluronic Acid Dressings	0:80	Graphene Oxide/Copper Nanoderivatives-Modified Chitosan/Hyaluronic Acid Dressings for Facilitating Wound Healing in Infected Full-Thickness Skin Defects.	0:152	Graphene Oxide/Copper Nanoderivatives-Modified Chitosan/Hyaluronic Acid Dressings for Facilitating Wound Healing in Infected Full-Thickness Skin Defects.
33149572	2	49	theme	high	506:509	arg1	risk					511:514	a high risk	504:514	a high risk of bacterial infection	504:537	This study aimed to manufacture a novel and multifunctional wound dressing by combining graphene oxide/copper nanocomposites (GO/Cu) with chitosan/hyaluronic acid, providing significant opportunities for the therapy of wound repair in wounds with a high risk of bacterial infection.
33149572	0	50	theme	Wound	99:103	arg1	Healing					105:111	Wound Healing	99:111	Wound Healing	99:111	Graphene Oxide/Copper Nanoderivatives-Modified Chitosan/Hyaluronic Acid Dressings for Facilitating Wound Healing in Infected Full-Thickness Skin Defects.
33149572	5	51	theme	C/H/GO/Cu	1250:1258	arg1	dressings					1260:1268	prepared C/H/GO/Cu dressings	1241:1268	prepared C/H/GO/Cu dressings	1241:1268	RESULTS Our experimental results indicated that the acidic environment facilitated the release of copper (CuNPs and Cu2+) from the dressings, and prepared C/H/GO/Cu dressings exhibited significant in vitro antimicrobial activities against the two tested bacterial strains (ATCC35984 and ATCC25923).
33149572	10	52	theme	intractable	2224:2234	arg1	wounds					2236:2241	intractable wounds	2224:2241	intractable wounds caused by bacterial infection	2224:2271	Thus, the multifunctional C/H/GO/Cu composite is expected to be a potential alternative for wound dressings, especially for the management of intractable wounds caused by bacterial infection.
33149572	3	53	theme	chitosan/hyaluronic	731:749	arg1	C/H					767:769	C/H	767:769	C/H	767:769	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	3	53	theme	chitosan/hyaluronic	731:749	arg1	dressings					817:825	GO-incorporated chitosan/hyaluronic acid dressings	776:825	GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO)	776:834	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	3	53	theme	chitosan/hyaluronic	731:749	arg1	dressings					756:764	chitosan/hyaluronic acid dressings	731:764	chitosan/hyaluronic acid dressings (C/H)	731:770	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	3	53	theme	chitosan/hyaluronic	731:749	arg1	controls					846:853	controls	846:853	controls	846:853	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	7	54	theme	granulation	1641:1651	arg1	tissues					1653:1659	granulation tissues	1641:1659	granulation tissues	1641:1659	Moreover, remarkably accelerated wound healing was found in the C/H/GO/Cu group, with controlled inflammatory infiltration and improved angiogenesis in granulation tissues.
33149572	2	55	from	therapy	465:471	arg1	wounds					492:497	wounds	492:497	wounds with a high risk of bacterial infection	492:537	This study aimed to manufacture a novel and multifunctional wound dressing by combining graphene oxide/copper nanocomposites (GO/Cu) with chitosan/hyaluronic acid, providing significant opportunities for the therapy of wound repair in wounds with a high risk of bacterial infection.
33149572	5	56	theme	bacterial	1349:1357	arg1	strains					1359:1365	the two tested bacterial strains	1334:1365	the two tested bacterial strains	1334:1365	RESULTS Our experimental results indicated that the acidic environment facilitated the release of copper (CuNPs and Cu2+) from the dressings, and prepared C/H/GO/Cu dressings exhibited significant in vitro antimicrobial activities against the two tested bacterial strains (ATCC35984 and ATCC25923).
33149572	5	57	theme	experimental	1107:1118	arg1	results					1120:1126	Our experimental results	1103:1126	Our experimental results	1103:1126	RESULTS Our experimental results indicated that the acidic environment facilitated the release of copper (CuNPs and Cu2+) from the dressings, and prepared C/H/GO/Cu dressings exhibited significant in vitro antimicrobial activities against the two tested bacterial strains (ATCC35984 and ATCC25923).
33149572	10	58	theme	bacterial	2253:2261	arg1	infection					2263:2271	bacterial infection	2253:2271	bacterial infection	2253:2271	Thus, the multifunctional C/H/GO/Cu composite is expected to be a potential alternative for wound dressings, especially for the management of intractable wounds caused by bacterial infection.
33149572	8	59	theme	organs	1747:1752	arg1	structures					1722:1731	the tissue structures	1711:1731	the tissue structures of the tested organs (heart, lung, liver and kidney) in any of the four groups	1711:1810	In addition, no pathological damage was noted in the tissue structures of the tested organs (heart, lung, liver and kidney) in any of the four groups.
33149572	2	60	theme	chitosan/hyaluronic	395:413	arg1	acid					415:418	chitosan/hyaluronic acid	395:418	chitosan/hyaluronic acid	395:418	This study aimed to manufacture a novel and multifunctional wound dressing by combining graphene oxide/copper nanocomposites (GO/Cu) with chitosan/hyaluronic acid, providing significant opportunities for the therapy of wound repair in wounds with a high risk of bacterial infection.
33149572	5	61	theme	antimicrobial	1301:1313	arg1	activities					1315:1324	significant in vitro antimicrobial activities	1280:1324	significant in vitro antimicrobial activities against the two tested bacterial strains	1280:1365	RESULTS Our experimental results indicated that the acidic environment facilitated the release of copper (CuNPs and Cu2+) from the dressings, and prepared C/H/GO/Cu dressings exhibited significant in vitro antimicrobial activities against the two tested bacterial strains (ATCC35984 and ATCC25923).
33149572	9	62	theme	accelerated	2025:2035	arg1	process					2045:2051	a significantly accelerated healing process	2009:2051	a significantly accelerated healing process of bacteria-infected wounds	2009:2079	CONCLUSION Collectively, GO/Cu-incorporated chitosan/hyaluronic acid dressings suggested a synergistic antimicrobial efficacy and acceptable biocompatibility both in vitro and in vivo, as well as a significantly accelerated healing process of bacteria-infected wounds.
33149572	3	63	theme	acid	599:602	arg1	C/H/GO/Cu					615:623	C/H/GO/Cu	615:623	C/H/GO/Cu	615:623	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	3	63	theme	acid	599:602	arg1	dressings					604:612	GO/Cu-decorated chitosan/hyaluronic acid dressings	563:612	GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu)	563:624	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	3	63	theme	acid	599:602	arg1	METHODS					540:546	METHODS	540:546	METHODS In this study	540:560	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	7	64	theme	inflammatory	1586:1597	arg1	infiltration					1599:1610	controlled inflammatory infiltration	1575:1610	controlled inflammatory infiltration	1575:1610	Moreover, remarkably accelerated wound healing was found in the C/H/GO/Cu group, with controlled inflammatory infiltration and improved angiogenesis in granulation tissues.
33149572	10	65	theme	C/H/GO/Cu	2108:2116	arg1	composite					2118:2126	the multifunctional C/H/GO/Cu composite	2088:2126	the multifunctional C/H/GO/Cu composite	2088:2126	Thus, the multifunctional C/H/GO/Cu composite is expected to be a potential alternative for wound dressings, especially for the management of intractable wounds caused by bacterial infection.
33149572	10	65	theme	C/H/GO/Cu	2108:2116	arg1	alternative					2158:2168	a potential alternative	2146:2168	a potential alternative for wound dressings, especially for the management of intractable wounds caused by bacterial infection	2146:2271	Thus, the multifunctional C/H/GO/Cu composite is expected to be a potential alternative for wound dressings, especially for the management of intractable wounds caused by bacterial infection.
33149572	3	66	theme	chitosan/hyaluronic	792:810	arg1	dressings					756:764	chitosan/hyaluronic acid dressings	731:764	chitosan/hyaluronic acid dressings (C/H)	731:770	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	3	66	theme	chitosan/hyaluronic	792:810	arg1	C/H/GO					828:833	C/H/GO	828:833	C/H/GO	828:833	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	3	66	theme	chitosan/hyaluronic	792:810	arg1	dressings					817:825	GO-incorporated chitosan/hyaluronic acid dressings	776:825	GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO)	776:834	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	3	66	theme	chitosan/hyaluronic	792:810	arg1	controls					846:853	controls	846:853	controls	846:853	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	2	67	theme	multifunctional	301:315	arg1	dressing					323:330	a novel and multifunctional wound dressing	289:330	a novel and multifunctional wound dressing	289:330	This study aimed to manufacture a novel and multifunctional wound dressing by combining graphene oxide/copper nanocomposites (GO/Cu) with chitosan/hyaluronic acid, providing significant opportunities for the therapy of wound repair in wounds with a high risk of bacterial infection.
33149572	7	68	theme	C/H/GO/Cu	1553:1561	arg1	group					1563:1567	the C/H/GO/Cu group	1549:1567	the C/H/GO/Cu group	1549:1567	Moreover, remarkably accelerated wound healing was found in the C/H/GO/Cu group, with controlled inflammatory infiltration and improved angiogenesis in granulation tissues.
33149572	9	69	theme	bacteria-infected	2056:2072	arg1	wounds					2074:2079	bacteria-infected wounds	2056:2079	bacteria-infected wounds	2056:2079	CONCLUSION Collectively, GO/Cu-incorporated chitosan/hyaluronic acid dressings suggested a synergistic antimicrobial efficacy and acceptable biocompatibility both in vitro and in vivo, as well as a significantly accelerated healing process of bacteria-infected wounds.
33149572	2	70	theme	oxide/copper	354:365	arg1	GO/Cu					383:387	GO/Cu	383:387	GO/Cu	383:387	This study aimed to manufacture a novel and multifunctional wound dressing by combining graphene oxide/copper nanocomposites (GO/Cu) with chitosan/hyaluronic acid, providing significant opportunities for the therapy of wound repair in wounds with a high risk of bacterial infection.
33149572	2	70	theme	oxide/copper	354:365	arg1	nanocomposites					367:380	graphene oxide/copper nanocomposites	345:380	graphene oxide/copper nanocomposites (GO/Cu)	345:388	This study aimed to manufacture a novel and multifunctional wound dressing by combining graphene oxide/copper nanocomposites (GO/Cu) with chitosan/hyaluronic acid, providing significant opportunities for the therapy of wound repair in wounds with a high risk of bacterial infection.
33149572	10	71	theme	wounds	2236:2241	arg1	management					2210:2219	the management	2206:2219	the management of intractable wounds caused by bacterial infection	2206:2271	Thus, the multifunctional C/H/GO/Cu composite is expected to be a potential alternative for wound dressings, especially for the management of intractable wounds caused by bacterial infection.
33149572	4	72	theme	pH	921:922	arg1	values					924:929	various pH values	913:929	various pH values	913:929	The surface characterization, in vitro degradation under various pH values, antimicrobial potential, cytocompatibility and in vivo therapeutic efficacy in a bacteria-infected full-thickness skin defect model were systematically evaluated.
33149572	5	73	dep	indicated	1128:1136	arg1	RESULTS					1095:1101	RESULTS	1095:1101	RESULTS	1095:1101	RESULTS Our experimental results indicated that the acidic environment facilitated the release of copper (CuNPs and Cu2+) from the dressings, and prepared C/H/GO/Cu dressings exhibited significant in vitro antimicrobial activities against the two tested bacterial strains (ATCC35984 and ATCC25923).
33149572	3	74	from	METHODS	540:546	arg1	study					556:560	this study	551:560	this study	551:560	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	7	75	from	infiltration	1599:1610	arg1	tissues					1653:1659	granulation tissues	1641:1659	granulation tissues	1641:1659	Moreover, remarkably accelerated wound healing was found in the C/H/GO/Cu group, with controlled inflammatory infiltration and improved angiogenesis in granulation tissues.
33149572	4	76	theme	therapeutic	987:997	arg1	efficacy					999:1006	in vivo therapeutic efficacy	979:1006	in vivo therapeutic efficacy	979:1006	The surface characterization, in vitro degradation under various pH values, antimicrobial potential, cytocompatibility and in vivo therapeutic efficacy in a bacteria-infected full-thickness skin defect model were systematically evaluated.
33149572	10	77	theme	multifunctional	2092:2106	arg1	composite					2118:2126	the multifunctional C/H/GO/Cu composite	2088:2126	the multifunctional C/H/GO/Cu composite	2088:2126	Thus, the multifunctional C/H/GO/Cu composite is expected to be a potential alternative for wound dressings, especially for the management of intractable wounds caused by bacterial infection.
33149572	10	77	theme	multifunctional	2092:2106	arg1	alternative					2158:2168	a potential alternative	2146:2168	a potential alternative for wound dressings, especially for the management of intractable wounds caused by bacterial infection	2146:2271	Thus, the multifunctional C/H/GO/Cu composite is expected to be a potential alternative for wound dressings, especially for the management of intractable wounds caused by bacterial infection.
33149572	6	78	theme	mouse	1457:1461	arg1	NIH/3T3-L1					1476:1485	NIH/3T3-L1	1476:1485	NIH/3T3-L1	1476:1485	All three dressings showed satisfactory cytocompatibility with mouse fibroblasts (NIH/3T3-L1).
33149572	6	78	theme	mouse	1457:1461	arg1	fibroblasts					1463:1473	mouse fibroblasts	1457:1473	mouse fibroblasts (NIH/3T3-L1)	1457:1486	All three dressings showed satisfactory cytocompatibility with mouse fibroblasts (NIH/3T3-L1).
33149572	3	79	theme	sodium	646:651	arg1	crosslinking					677:688	sodium trimetaphosphate (STMP) crosslinking	646:688	sodium trimetaphosphate (STMP) crosslinking	646:688	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	1	80	theme	plastic	240:246	arg1	surgery					248:254	plastic surgery	240:254	plastic surgery	240:254	PURPOSE Wound healing, especially of infected wounds, remains a clinical challenge in plastic surgery.
33149572	7	81	theme	wound	1522:1526	arg1	healing					1528:1534	remarkably accelerated wound healing	1499:1534	remarkably accelerated wound healing	1499:1534	Moreover, remarkably accelerated wound healing was found in the C/H/GO/Cu group, with controlled inflammatory infiltration and improved angiogenesis in granulation tissues.
33149572	9	82	theme	synergistic	1904:1914	arg1	efficacy					1930:1937	a synergistic antimicrobial efficacy	1902:1937	a synergistic antimicrobial efficacy	1902:1937	CONCLUSION Collectively, GO/Cu-incorporated chitosan/hyaluronic acid dressings suggested a synergistic antimicrobial efficacy and acceptable biocompatibility both in vitro and in vivo, as well as a significantly accelerated healing process of bacteria-infected wounds.
33149572	1	83	theme	PURPOSE	154:160	arg1	healing					168:174	PURPOSE Wound healing	154:174	PURPOSE Wound healing	154:174	PURPOSE Wound healing, especially of infected wounds, remains a clinical challenge in plastic surgery.
33149572	2	84	theme	novel	291:295	arg1	dressing					323:330	a novel and multifunctional wound dressing	289:330	a novel and multifunctional wound dressing	289:330	This study aimed to manufacture a novel and multifunctional wound dressing by combining graphene oxide/copper nanocomposites (GO/Cu) with chitosan/hyaluronic acid, providing significant opportunities for the therapy of wound repair in wounds with a high risk of bacterial infection.
33149572	2	85	with	wounds	492:497	arg1	risk					511:514	a high risk	504:514	a high risk of bacterial infection	504:537	This study aimed to manufacture a novel and multifunctional wound dressing by combining graphene oxide/copper nanocomposites (GO/Cu) with chitosan/hyaluronic acid, providing significant opportunities for the therapy of wound repair in wounds with a high risk of bacterial infection.
33149572	4	86	theme	in	979:980	arg1	efficacy					999:1006	in vivo therapeutic efficacy	979:1006	in vivo therapeutic efficacy	979:1006	The surface characterization, in vitro degradation under various pH values, antimicrobial potential, cytocompatibility and in vivo therapeutic efficacy in a bacteria-infected full-thickness skin defect model were systematically evaluated.
33149572	4	87	from	cytocompatibility	957:973	arg1	model					1058:1062	a bacteria-infected full-thickness skin defect model	1011:1062	a bacteria-infected full-thickness skin defect model	1011:1062	The surface characterization, in vitro degradation under various pH values, antimicrobial potential, cytocompatibility and in vivo therapeutic efficacy in a bacteria-infected full-thickness skin defect model were systematically evaluated.
33149572	5	88	dep	in	1292:1293	arg1	vitro					1295:1299	vitro	1295:1299	vitro	1295:1299	RESULTS Our experimental results indicated that the acidic environment facilitated the release of copper (CuNPs and Cu2+) from the dressings, and prepared C/H/GO/Cu dressings exhibited significant in vitro antimicrobial activities against the two tested bacterial strains (ATCC35984 and ATCC25923).
33149572	8	89	theme	tested	1740:1745	arg1	organs					1747:1752	the tested organs	1736:1752	the tested organs (heart, lung, liver and kidney)	1736:1784	In addition, no pathological damage was noted in the tissue structures of the tested organs (heart, lung, liver and kidney) in any of the four groups.
33149572	8	89	theme	tested	1740:1745	arg1	kidney					1778:1783	kidney	1778:1783	kidney	1778:1783	In addition, no pathological damage was noted in the tissue structures of the tested organs (heart, lung, liver and kidney) in any of the four groups.
33149572	8	89	theme	tested	1740:1745	arg1	liver					1768:1772	liver	1768:1772	liver	1768:1772	In addition, no pathological damage was noted in the tissue structures of the tested organs (heart, lung, liver and kidney) in any of the four groups.
33149572	8	89	theme	tested	1740:1745	arg1	lung					1762:1765	lung	1762:1765	lung	1762:1765	In addition, no pathological damage was noted in the tissue structures of the tested organs (heart, lung, liver and kidney) in any of the four groups.
33149572	8	89	theme	tested	1740:1745	arg1	heart					1755:1759	heart	1755:1759	heart	1755:1759	In addition, no pathological damage was noted in the tissue structures of the tested organs (heart, lung, liver and kidney) in any of the four groups.
33149572	9	90	theme	antimicrobial	1916:1928	arg1	efficacy					1930:1937	a synergistic antimicrobial efficacy	1902:1937	a synergistic antimicrobial efficacy	1902:1937	CONCLUSION Collectively, GO/Cu-incorporated chitosan/hyaluronic acid dressings suggested a synergistic antimicrobial efficacy and acceptable biocompatibility both in vitro and in vivo, as well as a significantly accelerated healing process of bacteria-infected wounds.
33149572	0	91	theme	Infected	116:123	arg1	Defects					145:151	Infected Full-Thickness Skin Defects	116:151	Infected Full-Thickness Skin Defects	116:151	Graphene Oxide/Copper Nanoderivatives-Modified Chitosan/Hyaluronic Acid Dressings for Facilitating Wound Healing in Infected Full-Thickness Skin Defects.
33149572	4	92	from	potential	946:954	arg1	model					1058:1062	a bacteria-infected full-thickness skin defect model	1011:1062	a bacteria-infected full-thickness skin defect model	1011:1062	The surface characterization, in vitro degradation under various pH values, antimicrobial potential, cytocompatibility and in vivo therapeutic efficacy in a bacteria-infected full-thickness skin defect model were systematically evaluated.
33149572	1	93	theme	infected	191:198	arg1	wounds					200:205	infected wounds	191:205	infected wounds	191:205	PURPOSE Wound healing, especially of infected wounds, remains a clinical challenge in plastic surgery.
33149572	2	94	theme	bacterial	519:527	arg1	infection					529:537	bacterial infection	519:537	bacterial infection	519:537	This study aimed to manufacture a novel and multifunctional wound dressing by combining graphene oxide/copper nanocomposites (GO/Cu) with chitosan/hyaluronic acid, providing significant opportunities for the therapy of wound repair in wounds with a high risk of bacterial infection.
33149572	5	95	dep	exhibited	1270:1278	arg1	ATCC25923					1382:1390	ATCC25923	1382:1390	ATCC25923	1382:1390	RESULTS Our experimental results indicated that the acidic environment facilitated the release of copper (CuNPs and Cu2+) from the dressings, and prepared C/H/GO/Cu dressings exhibited significant in vitro antimicrobial activities against the two tested bacterial strains (ATCC35984 and ATCC25923).
33149572	5	95	dep	exhibited	1270:1278	arg1	ATCC35984					1368:1376	ATCC35984	1368:1376	ATCC35984	1368:1376	RESULTS Our experimental results indicated that the acidic environment facilitated the release of copper (CuNPs and Cu2+) from the dressings, and prepared C/H/GO/Cu dressings exhibited significant in vitro antimicrobial activities against the two tested bacterial strains (ATCC35984 and ATCC25923).
33149572	0	96	theme	Skin	140:143	arg1	Defects					145:151	Infected Full-Thickness Skin Defects	116:151	Infected Full-Thickness Skin Defects	116:151	Graphene Oxide/Copper Nanoderivatives-Modified Chitosan/Hyaluronic Acid Dressings for Facilitating Wound Healing in Infected Full-Thickness Skin Defects.
33149572	4	97	theme	bacteria-infected	1013:1029	arg1	model					1058:1062	a bacteria-infected full-thickness skin defect model	1011:1062	a bacteria-infected full-thickness skin defect model	1011:1062	The surface characterization, in vitro degradation under various pH values, antimicrobial potential, cytocompatibility and in vivo therapeutic efficacy in a bacteria-infected full-thickness skin defect model were systematically evaluated.
33149572	8	98	dep	organs	1747:1752	arg1	kidney					1778:1783	kidney	1778:1783	kidney	1778:1783	In addition, no pathological damage was noted in the tissue structures of the tested organs (heart, lung, liver and kidney) in any of the four groups.
33149572	8	98	dep	organs	1747:1752	arg1	organs					1747:1752	the tested organs	1736:1752	the tested organs (heart, lung, liver and kidney)	1736:1784	In addition, no pathological damage was noted in the tissue structures of the tested organs (heart, lung, liver and kidney) in any of the four groups.
33149572	8	98	dep	organs	1747:1752	arg1	liver					1768:1772	liver	1768:1772	liver	1768:1772	In addition, no pathological damage was noted in the tissue structures of the tested organs (heart, lung, liver and kidney) in any of the four groups.
33149572	8	98	dep	organs	1747:1752	arg1	lung					1762:1765	lung	1762:1765	lung	1762:1765	In addition, no pathological damage was noted in the tissue structures of the tested organs (heart, lung, liver and kidney) in any of the four groups.
33149572	8	98	dep	organs	1747:1752	arg1	heart					1755:1759	heart	1755:1759	heart	1755:1759	In addition, no pathological damage was noted in the tissue structures of the tested organs (heart, lung, liver and kidney) in any of the four groups.
33149572	4	99	theme	skin	1046:1049	arg1	model					1058:1062	a bacteria-infected full-thickness skin defect model	1011:1062	a bacteria-infected full-thickness skin defect model	1011:1062	The surface characterization, in vitro degradation under various pH values, antimicrobial potential, cytocompatibility and in vivo therapeutic efficacy in a bacteria-infected full-thickness skin defect model were systematically evaluated.
33149572	8	100	theme	pathological	1678:1689	arg1	damage					1691:1696	no pathological damage	1675:1696	no pathological damage	1675:1696	In addition, no pathological damage was noted in the tissue structures of the tested organs (heart, lung, liver and kidney) in any of the four groups.
33149572	0	101	theme	Oxide/Copper	9:20	arg1	Dressings					72:80	Graphene Oxide/Copper Nanoderivatives-Modified Chitosan/Hyaluronic Acid Dressings	0:80	Graphene Oxide/Copper Nanoderivatives-Modified Chitosan/Hyaluronic Acid Dressings for Facilitating Wound Healing in Infected Full-Thickness Skin Defects.	0:152	Graphene Oxide/Copper Nanoderivatives-Modified Chitosan/Hyaluronic Acid Dressings for Facilitating Wound Healing in Infected Full-Thickness Skin Defects.
33149572	0	102	theme	Chitosan/Hyaluronic	47:65	arg1	Dressings					72:80	Graphene Oxide/Copper Nanoderivatives-Modified Chitosan/Hyaluronic Acid Dressings	0:80	Graphene Oxide/Copper Nanoderivatives-Modified Chitosan/Hyaluronic Acid Dressings for Facilitating Wound Healing in Infected Full-Thickness Skin Defects.	0:152	Graphene Oxide/Copper Nanoderivatives-Modified Chitosan/Hyaluronic Acid Dressings for Facilitating Wound Healing in Infected Full-Thickness Skin Defects.
33149572	7	103	located	found	1540:1544	arg2	healing					1528:1534	remarkably accelerated wound healing	1499:1534	remarkably accelerated wound healing	1499:1534	Moreover, remarkably accelerated wound healing was found in the C/H/GO/Cu group, with controlled inflammatory infiltration and improved angiogenesis in granulation tissues.
33149572	7	103	located	found	1540:1544	arg1	group					1563:1567	the C/H/GO/Cu group	1549:1567	the C/H/GO/Cu group	1549:1567	Moreover, remarkably accelerated wound healing was found in the C/H/GO/Cu group, with controlled inflammatory infiltration and improved angiogenesis in granulation tissues.
33149572	2	104	theme	repair	482:487	arg1	therapy					465:471	the therapy	461:471	the therapy of wound repair in wounds with a high risk of bacterial infection	461:537	This study aimed to manufacture a novel and multifunctional wound dressing by combining graphene oxide/copper nanocomposites (GO/Cu) with chitosan/hyaluronic acid, providing significant opportunities for the therapy of wound repair in wounds with a high risk of bacterial infection.
33149572	3	105	theme	vacuum	698:703	arg1	method					719:724	the vacuum freeze-drying method	694:724	the vacuum freeze-drying method	694:724	METHODS In this study, GO/Cu-decorated chitosan/hyaluronic acid dressings (C/H/GO/Cu) were prepared using sodium trimetaphosphate (STMP) crosslinking and the vacuum freeze-drying method, and chitosan/hyaluronic acid dressings (C/H) and GO-incorporated chitosan/hyaluronic acid dressings (C/H/GO) served as controls.
33149572	5	106	from	dressings	1226:1234	arg1	release					1182:1188	the release	1178:1188	the release of copper (CuNPs and Cu2+) from the dressings	1178:1234	RESULTS Our experimental results indicated that the acidic environment facilitated the release of copper (CuNPs and Cu2+) from the dressings, and prepared C/H/GO/Cu dressings exhibited significant in vitro antimicrobial activities against the two tested bacterial strains (ATCC35984 and ATCC25923).
33149572	4	107	dep	in	886:887	arg1	vitro					889:893	vitro	889:893	vitro	889:893	The surface characterization, in vitro degradation under various pH values, antimicrobial potential, cytocompatibility and in vivo therapeutic efficacy in a bacteria-infected full-thickness skin defect model were systematically evaluated.
33149572	8	108	theme	tissue	1715:1720	arg1	structures					1722:1731	the tissue structures	1711:1731	the tissue structures of the tested organs (heart, lung, liver and kidney) in any of the four groups	1711:1810	In addition, no pathological damage was noted in the tissue structures of the tested organs (heart, lung, liver and kidney) in any of the four groups.
33149572	5	109	theme	prepared	1241:1248	arg1	dressings					1260:1268	prepared C/H/GO/Cu dressings	1241:1268	prepared C/H/GO/Cu dressings	1241:1268	RESULTS Our experimental results indicated that the acidic environment facilitated the release of copper (CuNPs and Cu2+) from the dressings, and prepared C/H/GO/Cu dressings exhibited significant in vitro antimicrobial activities against the two tested bacterial strains (ATCC35984 and ATCC25923).
33149572	5	110	theme	copper	1193:1198	arg1	release					1182:1188	the release	1178:1188	the release of copper (CuNPs and Cu2+) from the dressings	1178:1234	RESULTS Our experimental results indicated that the acidic environment facilitated the release of copper (CuNPs and Cu2+) from the dressings, and prepared C/H/GO/Cu dressings exhibited significant in vitro antimicrobial activities against the two tested bacterial strains (ATCC35984 and ATCC25923).
33149572	5	111	dep	copper	1193:1198	arg1	Cu2+					1211:1214	Cu2+	1211:1214	Cu2+	1211:1214	RESULTS Our experimental results indicated that the acidic environment facilitated the release of copper (CuNPs and Cu2+) from the dressings, and prepared C/H/GO/Cu dressings exhibited significant in vitro antimicrobial activities against the two tested bacterial strains (ATCC35984 and ATCC25923).
33149572	5	111	dep	copper	1193:1198	arg1	CuNPs					1201:1205	CuNPs	1201:1205	CuNPs	1201:1205	RESULTS Our experimental results indicated that the acidic environment facilitated the release of copper (CuNPs and Cu2+) from the dressings, and prepared C/H/GO/Cu dressings exhibited significant in vitro antimicrobial activities against the two tested bacterial strains (ATCC35984 and ATCC25923).
33149572	5	112	theme	tested	1342:1347	arg1	strains					1359:1365	the two tested bacterial strains	1334:1365	the two tested bacterial strains	1334:1365	RESULTS Our experimental results indicated that the acidic environment facilitated the release of copper (CuNPs and Cu2+) from the dressings, and prepared C/H/GO/Cu dressings exhibited significant in vitro antimicrobial activities against the two tested bacterial strains (ATCC35984 and ATCC25923).
33149572	4	113	theme	surface	860:866	arg1	characterization					868:883	The surface characterization	856:883	The surface characterization	856:883	The surface characterization, in vitro degradation under various pH values, antimicrobial potential, cytocompatibility and in vivo therapeutic efficacy in a bacteria-infected full-thickness skin defect model were systematically evaluated.
32088225	0	0	theme	Mg	106:107	arg1	alloy					109:113	Mg alloy	106:113	Mg alloy	106:113	Characterization and preparation of Fe3O4 nanoparticles loaded bioglass-chitosan nanocomposite coating on Mg alloy and in vitro bioactivity assessment.
32088225	8	1	theme	corrosion	1221:1229	arg1	resistance					1231:1240	superior corrosion resistance	1212:1240	superior corrosion resistance	1212:1240	The corrosion analysis revealed that the BG-1% Fe3O4 nanocomposite coating has superior corrosion resistance as compared to the other coatings.
32088225	9	2	theme	research	1297:1304	arg1	findings					1281:1288	The findings	1277:1288	The findings of the research	1277:1304	The findings of the research have shown that the BG-Fe3O4-CS nanocomposite coating can be widely used as a suitable material for orthopedic applications.
32088225	4	3	theme	bioactive	638:646	arg1	coating					654:660	the bioactive glass coating	634:660	the bioactive glass coating	634:660	The experiment was performed with three varying concentrations (1, 3, 5 (wt%)) of Fe3O4 nanoparticles prepared by the co- precipitation method in the bioactive glass coating.
32088225	6	4	theme	composite	960:968	arg1	coatings					970:977	the composite coatings	956:977	the composite coatings	956:977	The bioactivity assessment was done in PBS for 7 days and it was detected that the composite coatings improve the bone-bonding ability which was again confirmed by SEM-EDX.
32088225	6	5	theme	bioactivity	881:891	arg1	assessment					893:902	The bioactivity assessment	877:902	The bioactivity assessment	877:902	The bioactivity assessment was done in PBS for 7 days and it was detected that the composite coatings improve the bone-bonding ability which was again confirmed by SEM-EDX.
32088225	5	6	theme	nanoparticles	862:874	arg1	increment					843:851	the progressive increment	827:851	the progressive increment of Fe3O4 nanoparticles	827:874	The antibacterial activity was examined in Escherichia coli and Staphylococcus aureus bacteria which determine that the growth of microorganisms was inhibited with the progressive increment of Fe3O4 nanoparticles.
32088225	1	7	theme	bioglass	167:174	arg1	coating					214:220	bioglass (BG)‑iron oxide (Fe3O4) nanocomposite coating	167:220	bioglass (BG)‑iron oxide (Fe3O4) nanocomposite coating	167:220	In this study, bioglass (BG)‑iron oxide (Fe3O4) nanocomposite coating was developed to produce bioactive and antibacterial coatings.
32088225	2	8	from	embedded	315:322	arg1	matrix					341:346	chitosan (CS) matrix	327:346	chitosan (CS) matrix	327:346	The nanocomposite coating was embedded in chitosan (CS) matrix and coating was fabricated by electrophoretic deposition (EPD) method.
32088225	9	9	theme	suitable	1384:1391	arg1	coating					1352:1358	the BG-Fe3O4-CS nanocomposite coating	1322:1358	the BG-Fe3O4-CS nanocomposite coating	1322:1358	The findings of the research have shown that the BG-Fe3O4-CS nanocomposite coating can be widely used as a suitable material for orthopedic applications.
32088225	9	9	theme	suitable	1384:1391	arg1	material					1393:1400	a suitable material	1382:1400	a suitable material for orthopedic applications	1382:1428	The findings of the research have shown that the BG-Fe3O4-CS nanocomposite coating can be widely used as a suitable material for orthopedic applications.
32088225	5	10	theme	antibacterial	667:679	arg1	activity					681:688	The antibacterial activity	663:688	The antibacterial activity	663:688	The antibacterial activity was examined in Escherichia coli and Staphylococcus aureus bacteria which determine that the growth of microorganisms was inhibited with the progressive increment of Fe3O4 nanoparticles.
32088225	8	11	theme	nanocomposite	1186:1198	arg1	coating					1200:1206	the BG-1% Fe3O4 nanocomposite coating	1170:1206	the BG-1% Fe3O4 nanocomposite coating	1170:1206	The corrosion analysis revealed that the BG-1% Fe3O4 nanocomposite coating has superior corrosion resistance as compared to the other coatings.
32088225	4	12	from	method	624:629	arg1	coating					654:660	the bioactive glass coating	634:660	the bioactive glass coating	634:660	The experiment was performed with three varying concentrations (1, 3, 5 (wt%)) of Fe3O4 nanoparticles prepared by the co- precipitation method in the bioactive glass coating.
32088225	1	13	theme	bioactive	247:255	arg1	coatings					275:282	bioactive and antibacterial coatings	247:282	bioactive and antibacterial coatings	247:282	In this study, bioglass (BG)‑iron oxide (Fe3O4) nanocomposite coating was developed to produce bioactive and antibacterial coatings.
32088225	9	14	used	used	1374:1377	arg2	material					1393:1400	a suitable material	1382:1400	a suitable material for orthopedic applications	1382:1428	The findings of the research have shown that the BG-Fe3O4-CS nanocomposite coating can be widely used as a suitable material for orthopedic applications.
32088225	9	14	used	used	1374:1377	arg2	coating					1352:1358	the BG-Fe3O4-CS nanocomposite coating	1322:1358	the BG-Fe3O4-CS nanocomposite coating	1322:1358	The findings of the research have shown that the BG-Fe3O4-CS nanocomposite coating can be widely used as a suitable material for orthopedic applications.
32088225	0	15	dep	in	119:120	arg1	vitro					122:126	vitro	122:126	vitro	122:126	Characterization and preparation of Fe3O4 nanoparticles loaded bioglass-chitosan nanocomposite coating on Mg alloy and in vitro bioactivity assessment.
32088225	5	16	theme	microorganisms	793:806	arg1	growth					783:788	the growth	779:788	the growth of microorganisms	779:806	The antibacterial activity was examined in Escherichia coli and Staphylococcus aureus bacteria which determine that the growth of microorganisms was inhibited with the progressive increment of Fe3O4 nanoparticles.
32088225	6	17	theme	bone-bonding	991:1002	arg1	ability					1004:1010	the bone-bonding ability	987:1010	the bone-bonding ability which was again confirmed by SEM-EDX	987:1047	The bioactivity assessment was done in PBS for 7 days and it was detected that the composite coatings improve the bone-bonding ability which was again confirmed by SEM-EDX.
32088225	5	18	theme	progressive	831:841	arg1	increment					843:851	the progressive increment	827:851	the progressive increment of Fe3O4 nanoparticles	827:874	The antibacterial activity was examined in Escherichia coli and Staphylococcus aureus bacteria which determine that the growth of microorganisms was inhibited with the progressive increment of Fe3O4 nanoparticles.
32088225	2	19	theme	electrophoretic	378:392	arg1	method					411:416	electrophoretic deposition (EPD) method	378:416	electrophoretic deposition (EPD) method	378:416	The nanocomposite coating was embedded in chitosan (CS) matrix and coating was fabricated by electrophoretic deposition (EPD) method.
32088225	0	20	theme	in	119:120	arg1	assessment					140:149	in vitro bioactivity assessment	119:149	in vitro bioactivity assessment	119:149	Characterization and preparation of Fe3O4 nanoparticles loaded bioglass-chitosan nanocomposite coating on Mg alloy and in vitro bioactivity assessment.
32088225	8	21	theme	Fe3O4	1180:1184	arg1	coating					1200:1206	the BG-1% Fe3O4 nanocomposite coating	1170:1206	the BG-1% Fe3O4 nanocomposite coating	1170:1206	The corrosion analysis revealed that the BG-1% Fe3O4 nanocomposite coating has superior corrosion resistance as compared to the other coatings.
32088225	2	22	from	matrix	341:346	arg1	coating					303:309	The nanocomposite coating	285:309	The nanocomposite coating	285:309	The nanocomposite coating was embedded in chitosan (CS) matrix and coating was fabricated by electrophoretic deposition (EPD) method.
32088225	2	22	from	matrix	341:346	arg1	embedded					315:322	embedded	315:322	embedded	315:322	The nanocomposite coating was embedded in chitosan (CS) matrix and coating was fabricated by electrophoretic deposition (EPD) method.
32088225	1	23	theme	BG	177:178	arg1	coating					214:220	bioglass (BG)‑iron oxide (Fe3O4) nanocomposite coating	167:220	bioglass (BG)‑iron oxide (Fe3O4) nanocomposite coating	167:220	In this study, bioglass (BG)‑iron oxide (Fe3O4) nanocomposite coating was developed to produce bioactive and antibacterial coatings.
32088225	4	24	dep	concentrations	536:549	arg1	5					558:558	5	558:558	5	558:558	The experiment was performed with three varying concentrations (1, 3, 5 (wt%)) of Fe3O4 nanoparticles prepared by the co- precipitation method in the bioactive glass coating.
32088225	8	25	contain	has	1208:1210	arg2	resistance					1231:1240	superior corrosion resistance	1212:1240	superior corrosion resistance	1212:1240	The corrosion analysis revealed that the BG-1% Fe3O4 nanocomposite coating has superior corrosion resistance as compared to the other coatings.
32088225	8	25	contain	has	1208:1210	arg1	coating					1200:1206	the BG-1% Fe3O4 nanocomposite coating	1170:1206	the BG-1% Fe3O4 nanocomposite coating	1170:1206	The corrosion analysis revealed that the BG-1% Fe3O4 nanocomposite coating has superior corrosion resistance as compared to the other coatings.
32088225	4	26	theme	precipitation	610:622	arg1	method					624:629	the co- precipitation method	602:629	the co- precipitation method in the bioactive glass coating	602:660	The experiment was performed with three varying concentrations (1, 3, 5 (wt%)) of Fe3O4 nanoparticles prepared by the co- precipitation method in the bioactive glass coating.
32088225	1	27	theme	antibacterial	261:273	arg1	coatings					275:282	bioactive and antibacterial coatings	247:282	bioactive and antibacterial coatings	247:282	In this study, bioglass (BG)‑iron oxide (Fe3O4) nanocomposite coating was developed to produce bioactive and antibacterial coatings.
32088225	7	28	theme	corrosion	1054:1062	arg1	behavior					1064:1071	The corrosion behavior	1050:1071	The corrosion behavior	1050:1071	The corrosion behavior was evaluated in Ringer's solution by electrochemical test.
32088225	1	29	theme	‑iron	180:184	arg1	coating					214:220	bioglass (BG)‑iron oxide (Fe3O4) nanocomposite coating	167:220	bioglass (BG)‑iron oxide (Fe3O4) nanocomposite coating	167:220	In this study, bioglass (BG)‑iron oxide (Fe3O4) nanocomposite coating was developed to produce bioactive and antibacterial coatings.
32088225	8	30	theme	BG-1	1174:1177	arg1	coating					1200:1206	the BG-1% Fe3O4 nanocomposite coating	1170:1206	the BG-1% Fe3O4 nanocomposite coating	1170:1206	The corrosion analysis revealed that the BG-1% Fe3O4 nanocomposite coating has superior corrosion resistance as compared to the other coatings.
32088225	8	31	theme	corrosion	1137:1145	arg1	analysis					1147:1154	The corrosion analysis	1133:1154	The corrosion analysis	1133:1154	The corrosion analysis revealed that the BG-1% Fe3O4 nanocomposite coating has superior corrosion resistance as compared to the other coatings.
32088225	5	32	dep	coli	718:721	arg1	bacteria					749:756	bacteria	749:756	bacteria	749:756	The antibacterial activity was examined in Escherichia coli and Staphylococcus aureus bacteria which determine that the growth of microorganisms was inhibited with the progressive increment of Fe3O4 nanoparticles.
32088225	9	33	theme	orthopedic	1406:1415	arg1	applications					1417:1428	orthopedic applications	1406:1428	orthopedic applications	1406:1428	The findings of the research have shown that the BG-Fe3O4-CS nanocomposite coating can be widely used as a suitable material for orthopedic applications.
32088225	1	34	theme	oxide	186:190	arg1	coating					214:220	bioglass (BG)‑iron oxide (Fe3O4) nanocomposite coating	167:220	bioglass (BG)‑iron oxide (Fe3O4) nanocomposite coating	167:220	In this study, bioglass (BG)‑iron oxide (Fe3O4) nanocomposite coating was developed to produce bioactive and antibacterial coatings.
32088225	0	35	theme	bioactivity	128:138	arg1	assessment					140:149	in vitro bioactivity assessment	119:149	in vitro bioactivity assessment	119:149	Characterization and preparation of Fe3O4 nanoparticles loaded bioglass-chitosan nanocomposite coating on Mg alloy and in vitro bioactivity assessment.
32088225	2	36	theme	nanocomposite	289:301	arg1	coating					303:309	The nanocomposite coating	285:309	The nanocomposite coating	285:309	The nanocomposite coating was embedded in chitosan (CS) matrix and coating was fabricated by electrophoretic deposition (EPD) method.
32088225	2	36	theme	nanocomposite	289:301	arg1	embedded					315:322	embedded	315:322	embedded	315:322	The nanocomposite coating was embedded in chitosan (CS) matrix and coating was fabricated by electrophoretic deposition (EPD) method.
32088225	2	37	theme	EPD	406:408	arg1	method					411:416	electrophoretic deposition (EPD) method	378:416	electrophoretic deposition (EPD) method	378:416	The nanocomposite coating was embedded in chitosan (CS) matrix and coating was fabricated by electrophoretic deposition (EPD) method.
32088225	7	38	theme	electrochemical	1111:1125	arg1	test					1127:1130	electrochemical test	1111:1130	electrochemical test	1111:1130	The corrosion behavior was evaluated in Ringer's solution by electrochemical test.
32088225	8	39	theme	other	1261:1265	arg1	coatings					1267:1274	the other coatings	1257:1274	the other coatings	1257:1274	The corrosion analysis revealed that the BG-1% Fe3O4 nanocomposite coating has superior corrosion resistance as compared to the other coatings.
32088225	4	40	dep	5	558:558	arg1	%					563:563	wt%	561:563	wt%	561:563	The experiment was performed with three varying concentrations (1, 3, 5 (wt%)) of Fe3O4 nanoparticles prepared by the co- precipitation method in the bioactive glass coating.
32088225	4	41	theme	co-	606:608	arg1	method					624:629	the co- precipitation method	602:629	the co- precipitation method in the bioactive glass coating	602:660	The experiment was performed with three varying concentrations (1, 3, 5 (wt%)) of Fe3O4 nanoparticles prepared by the co- precipitation method in the bioactive glass coating.
32088225	1	42	theme	Fe3O4	193:197	arg1	coating					214:220	bioglass (BG)‑iron oxide (Fe3O4) nanocomposite coating	167:220	bioglass (BG)‑iron oxide (Fe3O4) nanocomposite coating	167:220	In this study, bioglass (BG)‑iron oxide (Fe3O4) nanocomposite coating was developed to produce bioactive and antibacterial coatings.
32088225	0	43	theme	Fe3O4	36:40	arg1	preparation					21:31	preparation	21:31	preparation	21:31	Characterization and preparation of Fe3O4 nanoparticles loaded bioglass-chitosan nanocomposite coating on Mg alloy and in vitro bioactivity assessment.
32088225	0	43	theme	Fe3O4	36:40	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and preparation of Fe3O4 nanoparticles loaded bioglass-chitosan nanocomposite coating on Mg alloy and in vitro bioactivity assessment.
32088225	9	44	theme	BG-Fe3O4-CS	1326:1336	arg1	material					1393:1400	a suitable material	1382:1400	a suitable material for orthopedic applications	1382:1428	The findings of the research have shown that the BG-Fe3O4-CS nanocomposite coating can be widely used as a suitable material for orthopedic applications.
32088225	9	44	theme	BG-Fe3O4-CS	1326:1336	arg1	coating					1352:1358	the BG-Fe3O4-CS nanocomposite coating	1322:1358	the BG-Fe3O4-CS nanocomposite coating	1322:1358	The findings of the research have shown that the BG-Fe3O4-CS nanocomposite coating can be widely used as a suitable material for orthopedic applications.
32088225	8	45	theme	%	1178:1178	arg1	coating					1200:1206	the BG-1% Fe3O4 nanocomposite coating	1170:1206	the BG-1% Fe3O4 nanocomposite coating	1170:1206	The corrosion analysis revealed that the BG-1% Fe3O4 nanocomposite coating has superior corrosion resistance as compared to the other coatings.
32088225	2	46	theme	deposition	394:403	arg1	method					411:416	electrophoretic deposition (EPD) method	378:416	electrophoretic deposition (EPD) method	378:416	The nanocomposite coating was embedded in chitosan (CS) matrix and coating was fabricated by electrophoretic deposition (EPD) method.
32088225	0	47	theme	bioglass-chitosan	63:79	arg1	nanocomposite					81:93	loaded bioglass-chitosan nanocomposite	56:93	loaded bioglass-chitosan nanocomposite coating on Mg alloy and in vitro bioactivity assessment	56:149	Characterization and preparation of Fe3O4 nanoparticles loaded bioglass-chitosan nanocomposite coating on Mg alloy and in vitro bioactivity assessment.
32088225	9	48	theme	nanocomposite	1338:1350	arg1	material					1393:1400	a suitable material	1382:1400	a suitable material for orthopedic applications	1382:1428	The findings of the research have shown that the BG-Fe3O4-CS nanocomposite coating can be widely used as a suitable material for orthopedic applications.
32088225	9	48	theme	nanocomposite	1338:1350	arg1	coating					1352:1358	the BG-Fe3O4-CS nanocomposite coating	1322:1358	the BG-Fe3O4-CS nanocomposite coating	1322:1358	The findings of the research have shown that the BG-Fe3O4-CS nanocomposite coating can be widely used as a suitable material for orthopedic applications.
32088225	0	49	theme	loaded	56:61	arg1	nanocomposite					81:93	loaded bioglass-chitosan nanocomposite	56:93	loaded bioglass-chitosan nanocomposite coating on Mg alloy and in vitro bioactivity assessment	56:149	Characterization and preparation of Fe3O4 nanoparticles loaded bioglass-chitosan nanocomposite coating on Mg alloy and in vitro bioactivity assessment.
32088225	4	50	theme	varying	528:534	arg1	concentrations					536:549	three varying concentrations	522:549	three varying concentrations (1, 3, 5 (wt%)) of Fe3O4 nanoparticles prepared by the co- precipitation method in the bioactive glass coating	522:660	The experiment was performed with three varying concentrations (1, 3, 5 (wt%)) of Fe3O4 nanoparticles prepared by the co- precipitation method in the bioactive glass coating.
32088225	4	51	theme	glass	648:652	arg1	coating					654:660	the bioactive glass coating	634:660	the bioactive glass coating	634:660	The experiment was performed with three varying concentrations (1, 3, 5 (wt%)) of Fe3O4 nanoparticles prepared by the co- precipitation method in the bioactive glass coating.
32088225	2	52	theme	chitosan	327:334	arg1	matrix					341:346	chitosan (CS) matrix	327:346	chitosan (CS) matrix	327:346	The nanocomposite coating was embedded in chitosan (CS) matrix and coating was fabricated by electrophoretic deposition (EPD) method.
32088225	1	53	theme	nanocomposite	200:212	arg1	coating					214:220	bioglass (BG)‑iron oxide (Fe3O4) nanocomposite coating	167:220	bioglass (BG)‑iron oxide (Fe3O4) nanocomposite coating	167:220	In this study, bioglass (BG)‑iron oxide (Fe3O4) nanocomposite coating was developed to produce bioactive and antibacterial coatings.
32088225	8	54	theme	superior	1212:1219	arg1	resistance					1231:1240	superior corrosion resistance	1212:1240	superior corrosion resistance	1212:1240	The corrosion analysis revealed that the BG-1% Fe3O4 nanocomposite coating has superior corrosion resistance as compared to the other coatings.
32088225	4	55	theme	wt	561:562	arg1	%					563:563	wt%	561:563	wt%	561:563	The experiment was performed with three varying concentrations (1, 3, 5 (wt%)) of Fe3O4 nanoparticles prepared by the co- precipitation method in the bioactive glass coating.
32088225	4	56	theme	nanoparticles	576:588	arg1	concentrations					536:549	three varying concentrations	522:549	three varying concentrations (1, 3, 5 (wt%)) of Fe3O4 nanoparticles prepared by the co- precipitation method in the bioactive glass coating	522:660	The experiment was performed with three varying concentrations (1, 3, 5 (wt%)) of Fe3O4 nanoparticles prepared by the co- precipitation method in the bioactive glass coating.
32088225	4	57	theme	Fe3O4	570:574	arg1	nanoparticles					576:588	Fe3O4 nanoparticles	570:588	Fe3O4 nanoparticles prepared by the co- precipitation method in the bioactive glass coating	570:660	The experiment was performed with three varying concentrations (1, 3, 5 (wt%)) of Fe3O4 nanoparticles prepared by the co- precipitation method in the bioactive glass coating.
32088225	5	58	theme	Fe3O4	856:860	arg1	nanoparticles					862:874	Fe3O4 nanoparticles	856:874	Fe3O4 nanoparticles	856:874	The antibacterial activity was examined in Escherichia coli and Staphylococcus aureus bacteria which determine that the growth of microorganisms was inhibited with the progressive increment of Fe3O4 nanoparticles.
32737298	7	0	theme	embedded	1118:1125	arg1	matrix					1111:1116	a matrix	1109:1116	a matrix embedded in a body, which can autonomously release biopharmaceuticals by sensing biomarker proteins	1109:1216	Such composite hydrogels would be promising as a matrix embedded in a body, which can autonomously release biopharmaceuticals by sensing biomarker proteins.
32737298	7	1	from	embedded	1118:1125	arg1	body					1132:1135	a body	1130:1135	a body	1130:1135	Such composite hydrogels would be promising as a matrix embedded in a body, which can autonomously release biopharmaceuticals by sensing biomarker proteins.
32737298	0	2	theme	systems	110:116	arg1	release					27:33	Protein-responsive protein release	0:33	Protein-responsive protein release of supramolecular/polymer hydrogel composite integrating enzyme activation systems.	0:117	Protein-responsive protein release of supramolecular/polymer hydrogel composite integrating enzyme activation systems.
32737298	5	3	theme	orthogonal	780:789	arg1	agarose					828:834	agarose	828:834	agarose	828:834	In situ imaging shows that the supramolecular/agarose hydrogel composite consists of orthogonal domains of supramolecular fibers and agarose, which play distinct roles in protein entrapment and mechanical stiffness, respectively.
32737298	5	3	theme	orthogonal	780:789	arg1	domains					791:797	orthogonal domains	780:797	orthogonal domains	780:797	In situ imaging shows that the supramolecular/agarose hydrogel composite consists of orthogonal domains of supramolecular fibers and agarose, which play distinct roles in protein entrapment and mechanical stiffness, respectively.
32737298	5	3	theme	orthogonal	780:789	arg1	fibers					817:822	supramolecular fibers	802:822	supramolecular fibers	802:822	In situ imaging shows that the supramolecular/agarose hydrogel composite consists of orthogonal domains of supramolecular fibers and agarose, which play distinct roles in protein entrapment and mechanical stiffness, respectively.
32737298	6	4	theme	controlled	993:1002	arg1	release					1004:1010	controlled release	993:1010	controlled release of the embedded RNase in response to an antibody	993:1059	Integrating the enzyme activation set with the composite allows for controlled release of the embedded RNase in response to an antibody.
32737298	1	5	from	biopharmaceuticals	202:219	arg1	diseases					232:239	several diseases	224:239	several diseases	224:239	Non-enzymatic proteins including antibodies function as biomarkers and are used as biopharmaceuticals in several diseases.
32737298	3	6	theme	supramolecular/agarose	419:440	arg1	composite					451:459	a supramolecular/agarose hydrogel composite	417:459	a supramolecular/agarose hydrogel composite that can release a protein in response to a non-enzymatic protein	417:525	Here, we describe a supramolecular/agarose hydrogel composite that can release a protein in response to a non-enzymatic protein.
32737298	6	7	theme	activation	948:957	arg1	set					959:961	the enzyme activation set	937:961	the enzyme activation set	937:961	Integrating the enzyme activation set with the composite allows for controlled release of the embedded RNase in response to an antibody.
32737298	5	8	theme	protein	866:872	arg1	entrapment					874:883	protein entrapment	866:883	protein entrapment	866:883	In situ imaging shows that the supramolecular/agarose hydrogel composite consists of orthogonal domains of supramolecular fibers and agarose, which play distinct roles in protein entrapment and mechanical stiffness, respectively.
32737298	4	9	theme	protein-responsive	544:561	arg1	system					563:568	A non-enzymatic protein-responsive system	528:568	A non-enzymatic protein-responsive system	528:568	A non-enzymatic protein-responsive system is developed by hybridization of an enzyme-sensitive supramolecular hydrogel with a protein-triggered enzyme activation set.
32737298	5	10	theme	supramolecular/agarose	726:747	arg1	composite					758:766	the supramolecular/agarose hydrogel composite	722:766	the supramolecular/agarose hydrogel composite	722:766	In situ imaging shows that the supramolecular/agarose hydrogel composite consists of orthogonal domains of supramolecular fibers and agarose, which play distinct roles in protein entrapment and mechanical stiffness, respectively.
32737298	6	11	theme	enzyme	941:946	arg1	set					959:961	the enzyme activation set	937:961	the enzyme activation set	937:961	Integrating the enzyme activation set with the composite allows for controlled release of the embedded RNase in response to an antibody.
32737298	1	12	used	used	194:197	arg2	biopharmaceuticals					202:219	biopharmaceuticals	202:219	biopharmaceuticals in several diseases	202:239	Non-enzymatic proteins including antibodies function as biomarkers and are used as biopharmaceuticals in several diseases.
32737298	1	12	used	used	194:197	arg2	proteins					133:140	Non-enzymatic proteins	119:140	Non-enzymatic proteins including antibodies	119:161	Non-enzymatic proteins including antibodies function as biomarkers and are used as biopharmaceuticals in several diseases.
32737298	1	12	used	used	194:197	arg2	biomarkers					175:184	biomarkers	175:184	biomarkers	175:184	Non-enzymatic proteins including antibodies function as biomarkers and are used as biopharmaceuticals in several diseases.
32737298	6	13	theme	RNase	1028:1032	arg1	release					1004:1010	controlled release	993:1010	controlled release of the embedded RNase in response to an antibody	993:1059	Integrating the enzyme activation set with the composite allows for controlled release of the embedded RNase in response to an antibody.
32737298	5	14	theme	hydrogel	749:756	arg1	composite					758:766	the supramolecular/agarose hydrogel composite	722:766	the supramolecular/agarose hydrogel composite	722:766	In situ imaging shows that the supramolecular/agarose hydrogel composite consists of orthogonal domains of supramolecular fibers and agarose, which play distinct roles in protein entrapment and mechanical stiffness, respectively.
32737298	1	15	theme	several	224:230	arg1	diseases					232:239	several diseases	224:239	several diseases	224:239	Non-enzymatic proteins including antibodies function as biomarkers and are used as biopharmaceuticals in several diseases.
32737298	0	16	theme	protein	19:25	arg1	release					27:33	Protein-responsive protein release	0:33	Protein-responsive protein release of supramolecular/polymer hydrogel composite integrating enzyme activation systems.	0:117	Protein-responsive protein release of supramolecular/polymer hydrogel composite integrating enzyme activation systems.
32737298	6	17	theme	embedded	1019:1026	arg1	RNase					1028:1032	the embedded RNase	1015:1032	the embedded RNase	1015:1032	Integrating the enzyme activation set with the composite allows for controlled release of the embedded RNase in response to an antibody.
32737298	4	18	theme	protein-triggered	654:670	arg1	set					690:692	a protein-triggered enzyme activation set	652:692	a protein-triggered enzyme activation set	652:692	A non-enzymatic protein-responsive system is developed by hybridization of an enzyme-sensitive supramolecular hydrogel with a protein-triggered enzyme activation set.
32737298	1	19	theme	Non-enzymatic	119:131	arg1	biomarkers					175:184	biomarkers	175:184	biomarkers	175:184	Non-enzymatic proteins including antibodies function as biomarkers and are used as biopharmaceuticals in several diseases.
32737298	1	19	theme	Non-enzymatic	119:131	arg1	proteins					133:140	Non-enzymatic proteins	119:140	Non-enzymatic proteins including antibodies	119:161	Non-enzymatic proteins including antibodies function as biomarkers and are used as biopharmaceuticals in several diseases.
32737298	1	19	theme	Non-enzymatic	119:131	arg1	biopharmaceuticals					202:219	biopharmaceuticals	202:219	biopharmaceuticals in several diseases	202:239	Non-enzymatic proteins including antibodies function as biomarkers and are used as biopharmaceuticals in several diseases.
32737298	0	20	theme	Protein-responsive	0:17	arg1	release					27:33	Protein-responsive protein release	0:33	Protein-responsive protein release of supramolecular/polymer hydrogel composite integrating enzyme activation systems.	0:117	Protein-responsive protein release of supramolecular/polymer hydrogel composite integrating enzyme activation systems.
32737298	2	21	contain	have	332:335	arg1	materials					266:274	Protein-responsive soft materials	242:274	Protein-responsive soft materials capable of the controlled release of drugs and proteins	242:330	Protein-responsive soft materials capable of the controlled release of drugs and proteins have potential for use in next-generation diagnosis and therapies.
32737298	2	21	contain	have	332:335	arg2	potential					337:345	potential	337:345	potential	337:345	Protein-responsive soft materials capable of the controlled release of drugs and proteins have potential for use in next-generation diagnosis and therapies.
32737298	5	22	theme	In	695:696	arg1	imaging					703:709	In situ imaging	695:709	In situ imaging	695:709	In situ imaging shows that the supramolecular/agarose hydrogel composite consists of orthogonal domains of supramolecular fibers and agarose, which play distinct roles in protein entrapment and mechanical stiffness, respectively.
32737298	3	23	theme	hydrogel	442:449	arg1	composite					451:459	a supramolecular/agarose hydrogel composite	417:459	a supramolecular/agarose hydrogel composite that can release a protein in response to a non-enzymatic protein	417:525	Here, we describe a supramolecular/agarose hydrogel composite that can release a protein in response to a non-enzymatic protein.
32737298	7	24	theme	Such	1062:1065	arg1	hydrogels					1077:1085	Such composite hydrogels	1062:1085	Such composite hydrogels	1062:1085	Such composite hydrogels would be promising as a matrix embedded in a body, which can autonomously release biopharmaceuticals by sensing biomarker proteins.
32737298	7	25	theme	composite	1067:1075	arg1	hydrogels					1077:1085	Such composite hydrogels	1062:1085	Such composite hydrogels	1062:1085	Such composite hydrogels would be promising as a matrix embedded in a body, which can autonomously release biopharmaceuticals by sensing biomarker proteins.
32737298	2	26	theme	next-generation	358:372	arg1	diagnosis					374:382	next-generation diagnosis	358:382	next-generation diagnosis	358:382	Protein-responsive soft materials capable of the controlled release of drugs and proteins have potential for use in next-generation diagnosis and therapies.
32737298	7	27	from	body	1132:1135	arg1	embedded					1118:1125	embedded	1118:1125	embedded	1118:1125	Such composite hydrogels would be promising as a matrix embedded in a body, which can autonomously release biopharmaceuticals by sensing biomarker proteins.
32737298	4	28	theme	activation	679:688	arg1	set					690:692	a protein-triggered enzyme activation set	652:692	a protein-triggered enzyme activation set	652:692	A non-enzymatic protein-responsive system is developed by hybridization of an enzyme-sensitive supramolecular hydrogel with a protein-triggered enzyme activation set.
32737298	0	29	theme	hydrogel	61:68	arg1	systems					110:116	supramolecular/polymer hydrogel composite integrating enzyme activation systems	38:116	supramolecular/polymer hydrogel composite integrating enzyme activation systems	38:116	Protein-responsive protein release of supramolecular/polymer hydrogel composite integrating enzyme activation systems.
32737298	4	30	theme	enzyme	672:677	arg1	set					690:692	a protein-triggered enzyme activation set	652:692	a protein-triggered enzyme activation set	652:692	A non-enzymatic protein-responsive system is developed by hybridization of an enzyme-sensitive supramolecular hydrogel with a protein-triggered enzyme activation set.
32737298	2	31	theme	release	302:308	arg1	capable					276:282	capable	276:282	capable	276:282	Protein-responsive soft materials capable of the controlled release of drugs and proteins have potential for use in next-generation diagnosis and therapies.
32737298	0	32	theme	supramolecular/polymer	38:59	arg1	systems					110:116	supramolecular/polymer hydrogel composite integrating enzyme activation systems	38:116	supramolecular/polymer hydrogel composite integrating enzyme activation systems	38:116	Protein-responsive protein release of supramolecular/polymer hydrogel composite integrating enzyme activation systems.
32737298	4	33	theme	supramolecular	623:636	arg1	hydrogel					638:645	an enzyme-sensitive supramolecular hydrogel	603:645	an enzyme-sensitive supramolecular hydrogel	603:645	A non-enzymatic protein-responsive system is developed by hybridization of an enzyme-sensitive supramolecular hydrogel with a protein-triggered enzyme activation set.
32737298	5	34	theme	distinct	848:855	arg1	roles					857:861	distinct roles	848:861	distinct roles	848:861	In situ imaging shows that the supramolecular/agarose hydrogel composite consists of orthogonal domains of supramolecular fibers and agarose, which play distinct roles in protein entrapment and mechanical stiffness, respectively.
32737298	4	35	theme	non-enzymatic	530:542	arg1	system					563:568	A non-enzymatic protein-responsive system	528:568	A non-enzymatic protein-responsive system	528:568	A non-enzymatic protein-responsive system is developed by hybridization of an enzyme-sensitive supramolecular hydrogel with a protein-triggered enzyme activation set.
32737298	2	36	theme	controlled	291:300	arg1	release					302:308	the controlled release	287:308	the controlled release of drugs and proteins	287:330	Protein-responsive soft materials capable of the controlled release of drugs and proteins have potential for use in next-generation diagnosis and therapies.
32737298	0	37	theme	integrating	80:90	arg1	systems					110:116	supramolecular/polymer hydrogel composite integrating enzyme activation systems	38:116	supramolecular/polymer hydrogel composite integrating enzyme activation systems	38:116	Protein-responsive protein release of supramolecular/polymer hydrogel composite integrating enzyme activation systems.
32737298	4	38	theme	enzyme-sensitive	606:621	arg1	hydrogel					638:645	an enzyme-sensitive supramolecular hydrogel	603:645	an enzyme-sensitive supramolecular hydrogel	603:645	A non-enzymatic protein-responsive system is developed by hybridization of an enzyme-sensitive supramolecular hydrogel with a protein-triggered enzyme activation set.
32737298	5	39	theme	supramolecular	802:815	arg1	fibers					817:822	supramolecular fibers	802:822	supramolecular fibers	802:822	In situ imaging shows that the supramolecular/agarose hydrogel composite consists of orthogonal domains of supramolecular fibers and agarose, which play distinct roles in protein entrapment and mechanical stiffness, respectively.
32737298	5	40	theme	mechanical	889:898	arg1	stiffness					900:908	mechanical stiffness	889:908	mechanical stiffness	889:908	In situ imaging shows that the supramolecular/agarose hydrogel composite consists of orthogonal domains of supramolecular fibers and agarose, which play distinct roles in protein entrapment and mechanical stiffness, respectively.
32737298	7	41	theme	biomarker	1199:1207	arg1	proteins					1209:1216	biomarker proteins	1199:1216	biomarker proteins	1199:1216	Such composite hydrogels would be promising as a matrix embedded in a body, which can autonomously release biopharmaceuticals by sensing biomarker proteins.
32737298	0	42	theme	composite	70:78	arg1	systems					110:116	supramolecular/polymer hydrogel composite integrating enzyme activation systems	38:116	supramolecular/polymer hydrogel composite integrating enzyme activation systems	38:116	Protein-responsive protein release of supramolecular/polymer hydrogel composite integrating enzyme activation systems.
32737298	5	43	theme	fibers	817:822	arg1	agarose					828:834	agarose	828:834	agarose	828:834	In situ imaging shows that the supramolecular/agarose hydrogel composite consists of orthogonal domains of supramolecular fibers and agarose, which play distinct roles in protein entrapment and mechanical stiffness, respectively.
32737298	5	43	theme	fibers	817:822	arg1	domains					791:797	orthogonal domains	780:797	orthogonal domains	780:797	In situ imaging shows that the supramolecular/agarose hydrogel composite consists of orthogonal domains of supramolecular fibers and agarose, which play distinct roles in protein entrapment and mechanical stiffness, respectively.
32737298	5	43	theme	fibers	817:822	arg1	fibers					817:822	supramolecular fibers	802:822	supramolecular fibers	802:822	In situ imaging shows that the supramolecular/agarose hydrogel composite consists of orthogonal domains of supramolecular fibers and agarose, which play distinct roles in protein entrapment and mechanical stiffness, respectively.
32737298	2	44	theme	capable	276:282	arg1	materials					266:274	Protein-responsive soft materials	242:274	Protein-responsive soft materials capable of the controlled release of drugs and proteins	242:330	Protein-responsive soft materials capable of the controlled release of drugs and proteins have potential for use in next-generation diagnosis and therapies.
32737298	4	45	theme	hydrogel	638:645	arg1	hybridization					586:598	hybridization	586:598	hybridization of an enzyme-sensitive supramolecular hydrogel with a protein-triggered enzyme activation set	586:692	A non-enzymatic protein-responsive system is developed by hybridization of an enzyme-sensitive supramolecular hydrogel with a protein-triggered enzyme activation set.
32737298	3	46	dep	protein	519:525	arg1	response					491:498	response	491:498	response	491:498	Here, we describe a supramolecular/agarose hydrogel composite that can release a protein in response to a non-enzymatic protein.
32737298	2	47	theme	proteins	323:330	arg1	release					302:308	the controlled release	287:308	the controlled release of drugs and proteins	287:330	Protein-responsive soft materials capable of the controlled release of drugs and proteins have potential for use in next-generation diagnosis and therapies.
32737298	4	48	with	hybridization	586:598	arg1	set					690:692	a protein-triggered enzyme activation set	652:692	a protein-triggered enzyme activation set	652:692	A non-enzymatic protein-responsive system is developed by hybridization of an enzyme-sensitive supramolecular hydrogel with a protein-triggered enzyme activation set.
32737298	5	49	theme	agarose	828:834	arg1	agarose					828:834	agarose	828:834	agarose	828:834	In situ imaging shows that the supramolecular/agarose hydrogel composite consists of orthogonal domains of supramolecular fibers and agarose, which play distinct roles in protein entrapment and mechanical stiffness, respectively.
32737298	5	49	theme	agarose	828:834	arg1	domains					791:797	orthogonal domains	780:797	orthogonal domains	780:797	In situ imaging shows that the supramolecular/agarose hydrogel composite consists of orthogonal domains of supramolecular fibers and agarose, which play distinct roles in protein entrapment and mechanical stiffness, respectively.
32737298	5	49	theme	agarose	828:834	arg1	fibers					817:822	supramolecular fibers	802:822	supramolecular fibers	802:822	In situ imaging shows that the supramolecular/agarose hydrogel composite consists of orthogonal domains of supramolecular fibers and agarose, which play distinct roles in protein entrapment and mechanical stiffness, respectively.
32737298	2	50	theme	soft	261:264	arg1	materials					266:274	Protein-responsive soft materials	242:274	Protein-responsive soft materials capable of the controlled release of drugs and proteins	242:330	Protein-responsive soft materials capable of the controlled release of drugs and proteins have potential for use in next-generation diagnosis and therapies.
32737298	2	51	from	use	351:353	arg1	therapies					388:396	therapies	388:396	therapies	388:396	Protein-responsive soft materials capable of the controlled release of drugs and proteins have potential for use in next-generation diagnosis and therapies.
32737298	2	51	from	use	351:353	arg1	diagnosis					374:382	next-generation diagnosis	358:382	next-generation diagnosis	358:382	Protein-responsive soft materials capable of the controlled release of drugs and proteins have potential for use in next-generation diagnosis and therapies.
32737298	0	52	theme	activation	99:108	arg1	systems					110:116	supramolecular/polymer hydrogel composite integrating enzyme activation systems	38:116	supramolecular/polymer hydrogel composite integrating enzyme activation systems	38:116	Protein-responsive protein release of supramolecular/polymer hydrogel composite integrating enzyme activation systems.
32737298	2	53	theme	Protein-responsive	242:259	arg1	materials					266:274	Protein-responsive soft materials	242:274	Protein-responsive soft materials capable of the controlled release of drugs and proteins	242:330	Protein-responsive soft materials capable of the controlled release of drugs and proteins have potential for use in next-generation diagnosis and therapies.
32737298	2	54	theme	drugs	313:317	arg1	release					302:308	the controlled release	287:308	the controlled release of drugs and proteins	287:330	Protein-responsive soft materials capable of the controlled release of drugs and proteins have potential for use in next-generation diagnosis and therapies.
32737298	3	55	theme	non-enzymatic	505:517	arg1	protein					519:525	a non-enzymatic protein	503:525	a non-enzymatic protein	503:525	Here, we describe a supramolecular/agarose hydrogel composite that can release a protein in response to a non-enzymatic protein.
32737298	0	56	theme	enzyme	92:97	arg1	systems					110:116	supramolecular/polymer hydrogel composite integrating enzyme activation systems	38:116	supramolecular/polymer hydrogel composite integrating enzyme activation systems	38:116	Protein-responsive protein release of supramolecular/polymer hydrogel composite integrating enzyme activation systems.
32737298	6	57	from	release	1004:1010	arg1	response					1037:1044	response	1037:1044	response to an antibody	1037:1059	Integrating the enzyme activation set with the composite allows for controlled release of the embedded RNase in response to an antibody.
32737298	5	58	dep	In	695:696	arg1	situ					698:701	situ	698:701	situ	698:701	In situ imaging shows that the supramolecular/agarose hydrogel composite consists of orthogonal domains of supramolecular fibers and agarose, which play distinct roles in protein entrapment and mechanical stiffness, respectively.
34450143	0	0	theme	cationic	110:117	arg1	dyes					119:122	cationic dyes	110:122	cationic dyes from water and wastewater	110:148	Preparation of clinoptilolite/starch/CoFe2O4 magnetic nanocomposite powder and its elimination properties for cationic dyes from water and wastewater.
34450143	7	1	theme	kinetic	1158:1164	arg1	model					1166:1170	a pseudo-first order (PFO) kinetic model	1131:1170	a pseudo-first order (PFO) kinetic model	1131:1170	In addition, the kinetic data of the removal process followed a pseudo-first order (PFO) kinetic model.
34450143	1	2	theme	clinoptilolite	180:193	arg1	/Starch/CoFe2O4					200:214	A new magnetic nanocomposite clinoptilolite (CLT)/Starch/CoFe2O4	151:214	A new magnetic nanocomposite clinoptilolite (CLT)/Starch/CoFe2O4	151:214	A new magnetic nanocomposite clinoptilolite (CLT)/Starch/CoFe2O4 was synthesized using co-precipitation method.
34450143	7	3	theme	kinetic	1086:1092	arg1	data					1094:1097	the kinetic data	1082:1097	the kinetic data of the removal process	1082:1120	In addition, the kinetic data of the removal process followed a pseudo-first order (PFO) kinetic model.
34450143	2	4	theme	dye	378:380	arg1	decontamination					319:333	decontamination	319:333	decontamination of methylene blue dye (MBD), methyl violet dye (MVD), and crystal violet dye (CVD) from water media	319:433	The prepared magnetic composite powder was utilized for decontamination of methylene blue dye (MBD), methyl violet dye (MVD), and crystal violet dye (CVD) from water media.
34450143	6	5	theme	MVD	962:964	arg1	32.84 mg.g-1					1009:1020	32.84 mg.g-1	1009:1020	32.84 mg.g-1	1009:1020	Also, the maximum adsorption capacity of CVD, MBD, and MVD to the desired composite was determined as 32.84 mg.g-1, 31.81 mg.g-1, and 31.15 mg.g-1, respectively.
34450143	6	5	theme	MVD	962:964	arg1	capacity					936:943	the maximum adsorption capacity	913:943	the maximum adsorption capacity of CVD, MBD, and MVD to the desired composite	913:989	Also, the maximum adsorption capacity of CVD, MBD, and MVD to the desired composite was determined as 32.84 mg.g-1, 31.81 mg.g-1, and 31.15 mg.g-1, respectively.
34450143	6	5	theme	MVD	962:964	arg1	31.81 mg.g-1					1023:1034	31.81 mg.g-1	1023:1034	31.81 mg.g-1	1023:1034	Also, the maximum adsorption capacity of CVD, MBD, and MVD to the desired composite was determined as 32.84 mg.g-1, 31.81 mg.g-1, and 31.15 mg.g-1, respectively.
34450143	6	5	theme	MVD	962:964	arg1	31.15 mg.g-1					1041:1052	31.15 mg.g-1	1041:1052	31.15 mg.g-1	1041:1052	Also, the maximum adsorption capacity of CVD, MBD, and MVD to the desired composite was determined as 32.84 mg.g-1, 31.81 mg.g-1, and 31.15 mg.g-1, respectively.
34450143	7	6	theme	PFO	1153:1155	arg1	model					1166:1170	a pseudo-first order (PFO) kinetic model	1131:1170	a pseudo-first order (PFO) kinetic model	1131:1170	In addition, the kinetic data of the removal process followed a pseudo-first order (PFO) kinetic model.
34450143	2	7	from	media	429:433	arg1	decontamination					319:333	decontamination	319:333	decontamination of methylene blue dye (MBD), methyl violet dye (MVD), and crystal violet dye (CVD) from water media	319:433	The prepared magnetic composite powder was utilized for decontamination of methylene blue dye (MBD), methyl violet dye (MVD), and crystal violet dye (CVD) from water media.
34450143	3	8	theme	specific	534:541	arg1	surface					543:549	its specific surface	530:549	its specific surface	530:549	The BET analysis showed that CLT modification using starch and CoFe2O4 nanoparticles improved its specific surface and the amount of specific surface area for CLT, CoFe2O4, and CLT/Starch/CoFe2O4 powder was reported to be 18.82 m2.g-1, 151.4 m2.g-1, and 104.75 m2.g-1, respectively.
34450143	3	9	dep	nanoparticles	507:519	arg1	improved					521:528	improved	521:528	nanoparticles improved its specific surface	507:549	The BET analysis showed that CLT modification using starch and CoFe2O4 nanoparticles improved its specific surface and the amount of specific surface area for CLT, CoFe2O4, and CLT/Starch/CoFe2O4 powder was reported to be 18.82 m2.g-1, 151.4 m2.g-1, and 104.75 m2.g-1, respectively.
34450143	9	10	theme	excellent	1374:1382	arg1	ability					1384:1390	an excellent ability	1371:1390	an excellent ability to adsorb cationic dyes after several consecutive cycles	1371:1447	Finally, ad(de)sorption experiments' results showed that the synthesized nanocomposite adsorbent has an excellent ability to adsorb cationic dyes after several consecutive cycles.
34450143	9	11	theme	sorption	1285:1292	arg1	results					1307:1313	ad(de)sorption experiments' results	1279:1313	ad(de)sorption experiments' results	1279:1313	Finally, ad(de)sorption experiments' results showed that the synthesized nanocomposite adsorbent has an excellent ability to adsorb cationic dyes after several consecutive cycles.
34450143	9	12	theme	consecutive	1430:1440	arg1	cycles					1442:1447	several consecutive cycles	1422:1447	several consecutive cycles	1422:1447	Finally, ad(de)sorption experiments' results showed that the synthesized nanocomposite adsorbent has an excellent ability to adsorb cationic dyes after several consecutive cycles.
34450143	3	13	theme	CLT	465:467	arg1	modification					469:480	CLT modification	465:480	CLT modification using starch and CoFe2O4	465:505	The BET analysis showed that CLT modification using starch and CoFe2O4 nanoparticles improved its specific surface and the amount of specific surface area for CLT, CoFe2O4, and CLT/Starch/CoFe2O4 powder was reported to be 18.82 m2.g-1, 151.4 m2.g-1, and 104.75 m2.g-1, respectively.
34450143	7	14	theme	order	1146:1150	arg1	model					1166:1170	a pseudo-first order (PFO) kinetic model	1131:1170	a pseudo-first order (PFO) kinetic model	1131:1170	In addition, the kinetic data of the removal process followed a pseudo-first order (PFO) kinetic model.
34450143	4	15	theme	vital	763:767	arg1	role					769:772	a vital role	761:772	a vital role	761:772	Experimental results showed that pH 9 had a vital role in the adsorption process of all three types.
34450143	2	16	theme	violet	401:406	arg1	CVD					413:415	CVD	413:415	CVD	413:415	The prepared magnetic composite powder was utilized for decontamination of methylene blue dye (MBD), methyl violet dye (MVD), and crystal violet dye (CVD) from water media.
34450143	2	16	theme	violet	401:406	arg1	dye					408:410	crystal violet dye	393:410	crystal violet dye (CVD)	393:416	The prepared magnetic composite powder was utilized for decontamination of methylene blue dye (MBD), methyl violet dye (MVD), and crystal violet dye (CVD) from water media.
34450143	5	17	theme	experimental	888:899	arg1	data					901:904	experimental data	888:904	experimental data	888:904	Langmuir and Redlich-Petersen isotherm models were well fitted with experimental data.
34450143	4	18	theme	types	813:817	arg1	process					792:798	the adsorption process	777:798	the adsorption process of all three types	777:817	Experimental results showed that pH 9 had a vital role in the adsorption process of all three types.
34450143	2	19	theme	crystal	393:399	arg1	CVD					413:415	CVD	413:415	CVD	413:415	The prepared magnetic composite powder was utilized for decontamination of methylene blue dye (MBD), methyl violet dye (MVD), and crystal violet dye (CVD) from water media.
34450143	2	19	theme	crystal	393:399	arg1	dye					408:410	crystal violet dye	393:410	crystal violet dye (CVD)	393:416	The prepared magnetic composite powder was utilized for decontamination of methylene blue dye (MBD), methyl violet dye (MVD), and crystal violet dye (CVD) from water media.
34450143	9	20	theme	de	1282:1283	arg1	results					1307:1313	ad(de)sorption experiments' results	1279:1313	ad(de)sorption experiments' results	1279:1313	Finally, ad(de)sorption experiments' results showed that the synthesized nanocomposite adsorbent has an excellent ability to adsorb cationic dyes after several consecutive cycles.
34450143	2	21	used	utilized	306:313	arg2	powder					295:300	The prepared magnetic composite powder	263:300	The prepared magnetic composite powder	263:300	The prepared magnetic composite powder was utilized for decontamination of methylene blue dye (MBD), methyl violet dye (MVD), and crystal violet dye (CVD) from water media.
34450143	9	22	theme	cationic	1402:1409	arg1	dyes					1411:1414	cationic dyes	1402:1414	cationic dyes after several consecutive cycles	1402:1447	Finally, ad(de)sorption experiments' results showed that the synthesized nanocomposite adsorbent has an excellent ability to adsorb cationic dyes after several consecutive cycles.
34450143	7	23	theme	pseudo-first	1133:1144	arg1	model					1166:1170	a pseudo-first order (PFO) kinetic model	1131:1170	a pseudo-first order (PFO) kinetic model	1131:1170	In addition, the kinetic data of the removal process followed a pseudo-first order (PFO) kinetic model.
34450143	6	24	theme	MBD	953:955	arg1	32.84 mg.g-1					1009:1020	32.84 mg.g-1	1009:1020	32.84 mg.g-1	1009:1020	Also, the maximum adsorption capacity of CVD, MBD, and MVD to the desired composite was determined as 32.84 mg.g-1, 31.81 mg.g-1, and 31.15 mg.g-1, respectively.
34450143	6	24	theme	MBD	953:955	arg1	capacity					936:943	the maximum adsorption capacity	913:943	the maximum adsorption capacity of CVD, MBD, and MVD to the desired composite	913:989	Also, the maximum adsorption capacity of CVD, MBD, and MVD to the desired composite was determined as 32.84 mg.g-1, 31.81 mg.g-1, and 31.15 mg.g-1, respectively.
34450143	6	24	theme	MBD	953:955	arg1	31.81 mg.g-1					1023:1034	31.81 mg.g-1	1023:1034	31.81 mg.g-1	1023:1034	Also, the maximum adsorption capacity of CVD, MBD, and MVD to the desired composite was determined as 32.84 mg.g-1, 31.81 mg.g-1, and 31.15 mg.g-1, respectively.
34450143	6	24	theme	MBD	953:955	arg1	31.15 mg.g-1					1041:1052	31.15 mg.g-1	1041:1052	31.15 mg.g-1	1041:1052	Also, the maximum adsorption capacity of CVD, MBD, and MVD to the desired composite was determined as 32.84 mg.g-1, 31.81 mg.g-1, and 31.15 mg.g-1, respectively.
34450143	5	25	theme	Redlich-Petersen	833:848	arg1	models					859:864	Langmuir and Redlich-Petersen isotherm models	820:864	Langmuir and Redlich-Petersen isotherm models	820:864	Langmuir and Redlich-Petersen isotherm models were well fitted with experimental data.
34450143	4	26	theme	Experimental	719:730	arg1	results					732:738	Experimental results	719:738	Experimental results	719:738	Experimental results showed that pH 9 had a vital role in the adsorption process of all three types.
34450143	9	27	theme	adsorbent	1357:1365	arg1	nanocomposite					1343:1355	the synthesized nanocomposite adsorbent	1327:1365	the synthesized nanocomposite adsorbent	1327:1365	Finally, ad(de)sorption experiments' results showed that the synthesized nanocomposite adsorbent has an excellent ability to adsorb cationic dyes after several consecutive cycles.
34450143	2	28	theme	water	423:427	arg1	media					429:433	water media	423:433	water media	423:433	The prepared magnetic composite powder was utilized for decontamination of methylene blue dye (MBD), methyl violet dye (MVD), and crystal violet dye (CVD) from water media.
34450143	9	29	contain	has	1367:1369	arg1	nanocomposite					1343:1355	the synthesized nanocomposite adsorbent	1327:1365	the synthesized nanocomposite adsorbent	1327:1365	Finally, ad(de)sorption experiments' results showed that the synthesized nanocomposite adsorbent has an excellent ability to adsorb cationic dyes after several consecutive cycles.
34450143	9	29	contain	has	1367:1369	arg2	ability					1384:1390	an excellent ability	1371:1390	an excellent ability to adsorb cationic dyes after several consecutive cycles	1371:1447	Finally, ad(de)sorption experiments' results showed that the synthesized nanocomposite adsorbent has an excellent ability to adsorb cationic dyes after several consecutive cycles.
34450143	2	30	theme	dye	353:355	arg1	decontamination					319:333	decontamination	319:333	decontamination of methylene blue dye (MBD), methyl violet dye (MVD), and crystal violet dye (CVD) from water media	319:433	The prepared magnetic composite powder was utilized for decontamination of methylene blue dye (MBD), methyl violet dye (MVD), and crystal violet dye (CVD) from water media.
34450143	3	31	theme	area	586:589	arg1	area					586:589	specific surface area	569:589	specific surface area for CLT, CoFe2O4, and CLT/Starch/CoFe2O4 powder	569:637	The BET analysis showed that CLT modification using starch and CoFe2O4 nanoparticles improved its specific surface and the amount of specific surface area for CLT, CoFe2O4, and CLT/Starch/CoFe2O4 powder was reported to be 18.82 m2.g-1, 151.4 m2.g-1, and 104.75 m2.g-1, respectively.
34450143	3	31	theme	area	586:589	arg1	amount					559:564	the amount	555:564	the amount of specific surface area for CLT, CoFe2O4, and CLT/Starch/CoFe2O4 powder	555:637	The BET analysis showed that CLT modification using starch and CoFe2O4 nanoparticles improved its specific surface and the amount of specific surface area for CLT, CoFe2O4, and CLT/Starch/CoFe2O4 powder was reported to be 18.82 m2.g-1, 151.4 m2.g-1, and 104.75 m2.g-1, respectively.
34450143	3	31	theme	area	586:589	arg1	18.82 m2.g-1					658:669	18.82 m2.g-1	658:669	18.82 m2.g-1	658:669	The BET analysis showed that CLT modification using starch and CoFe2O4 nanoparticles improved its specific surface and the amount of specific surface area for CLT, CoFe2O4, and CLT/Starch/CoFe2O4 powder was reported to be 18.82 m2.g-1, 151.4 m2.g-1, and 104.75 m2.g-1, respectively.
34450143	0	32	theme	clinoptilolite/starch/CoFe2O4	15:43	arg1	powder					68:73	clinoptilolite/starch/CoFe2O4 magnetic nanocomposite powder	15:73	clinoptilolite/starch/CoFe2O4 magnetic nanocomposite powder	15:73	Preparation of clinoptilolite/starch/CoFe2O4 magnetic nanocomposite powder and its elimination properties for cationic dyes from water and wastewater.
34450143	6	33	theme	CVD	948:950	arg1	32.84 mg.g-1					1009:1020	32.84 mg.g-1	1009:1020	32.84 mg.g-1	1009:1020	Also, the maximum adsorption capacity of CVD, MBD, and MVD to the desired composite was determined as 32.84 mg.g-1, 31.81 mg.g-1, and 31.15 mg.g-1, respectively.
34450143	6	33	theme	CVD	948:950	arg1	capacity					936:943	the maximum adsorption capacity	913:943	the maximum adsorption capacity of CVD, MBD, and MVD to the desired composite	913:989	Also, the maximum adsorption capacity of CVD, MBD, and MVD to the desired composite was determined as 32.84 mg.g-1, 31.81 mg.g-1, and 31.15 mg.g-1, respectively.
34450143	6	33	theme	CVD	948:950	arg1	31.81 mg.g-1					1023:1034	31.81 mg.g-1	1023:1034	31.81 mg.g-1	1023:1034	Also, the maximum adsorption capacity of CVD, MBD, and MVD to the desired composite was determined as 32.84 mg.g-1, 31.81 mg.g-1, and 31.15 mg.g-1, respectively.
34450143	6	33	theme	CVD	948:950	arg1	31.15 mg.g-1					1041:1052	31.15 mg.g-1	1041:1052	31.15 mg.g-1	1041:1052	Also, the maximum adsorption capacity of CVD, MBD, and MVD to the desired composite was determined as 32.84 mg.g-1, 31.81 mg.g-1, and 31.15 mg.g-1, respectively.
34450143	2	34	theme	blue	348:351	arg1	dye					353:355	methylene blue dye	338:355	methylene blue dye (MBD)	338:361	The prepared magnetic composite powder was utilized for decontamination of methylene blue dye (MBD), methyl violet dye (MVD), and crystal violet dye (CVD) from water media.
34450143	2	34	theme	blue	348:351	arg1	MBD					358:360	MBD	358:360	MBD	358:360	The prepared magnetic composite powder was utilized for decontamination of methylene blue dye (MBD), methyl violet dye (MVD), and crystal violet dye (CVD) from water media.
34450143	9	35	theme	several	1422:1428	arg1	cycles					1442:1447	several consecutive cycles	1422:1447	several consecutive cycles	1422:1447	Finally, ad(de)sorption experiments' results showed that the synthesized nanocomposite adsorbent has an excellent ability to adsorb cationic dyes after several consecutive cycles.
34450143	0	36	theme	powder	68:73	arg1	Preparation					0:10	Preparation	0:10	Preparation of clinoptilolite/starch/CoFe2O4 magnetic nanocomposite powder and its elimination properties for cationic dyes from water and wastewater.	0:149	Preparation of clinoptilolite/starch/CoFe2O4 magnetic nanocomposite powder and its elimination properties for cationic dyes from water and wastewater.
34450143	5	37	theme	Langmuir	820:827	arg1	models					859:864	Langmuir and Redlich-Petersen isotherm models	820:864	Langmuir and Redlich-Petersen isotherm models	820:864	Langmuir and Redlich-Petersen isotherm models were well fitted with experimental data.
34450143	2	38	theme	magnetic	276:283	arg1	powder					295:300	The prepared magnetic composite powder	263:300	The prepared magnetic composite powder	263:300	The prepared magnetic composite powder was utilized for decontamination of methylene blue dye (MBD), methyl violet dye (MVD), and crystal violet dye (CVD) from water media.
34450143	1	39	theme	co-precipitation	238:253	arg1	method					255:260	co-precipitation method	238:260	co-precipitation method	238:260	A new magnetic nanocomposite clinoptilolite (CLT)/Starch/CoFe2O4 was synthesized using co-precipitation method.
34450143	0	40	theme	nanocomposite	54:66	arg1	powder					68:73	clinoptilolite/starch/CoFe2O4 magnetic nanocomposite powder	15:73	clinoptilolite/starch/CoFe2O4 magnetic nanocomposite powder	15:73	Preparation of clinoptilolite/starch/CoFe2O4 magnetic nanocomposite powder and its elimination properties for cationic dyes from water and wastewater.
34450143	7	41	theme	removal	1106:1112	arg1	process					1114:1120	the removal process	1102:1120	the removal process	1102:1120	In addition, the kinetic data of the removal process followed a pseudo-first order (PFO) kinetic model.
34450143	0	42	theme	magnetic	45:52	arg1	powder					68:73	clinoptilolite/starch/CoFe2O4 magnetic nanocomposite powder	15:73	clinoptilolite/starch/CoFe2O4 magnetic nanocomposite powder	15:73	Preparation of clinoptilolite/starch/CoFe2O4 magnetic nanocomposite powder and its elimination properties for cationic dyes from water and wastewater.
34450143	2	43	theme	dye	408:410	arg1	decontamination					319:333	decontamination	319:333	decontamination of methylene blue dye (MBD), methyl violet dye (MVD), and crystal violet dye (CVD) from water media	319:433	The prepared magnetic composite powder was utilized for decontamination of methylene blue dye (MBD), methyl violet dye (MVD), and crystal violet dye (CVD) from water media.
34450143	8	44	theme	Negative	1173:1180	arg1	parameters					1196:1205	Negative thermodynamic parameters	1173:1205	Negative thermodynamic parameters	1173:1205	Negative thermodynamic parameters were indicated that the process is spontaneous and exothermic.
34450143	6	45	theme	adsorption	925:934	arg1	32.84 mg.g-1					1009:1020	32.84 mg.g-1	1009:1020	32.84 mg.g-1	1009:1020	Also, the maximum adsorption capacity of CVD, MBD, and MVD to the desired composite was determined as 32.84 mg.g-1, 31.81 mg.g-1, and 31.15 mg.g-1, respectively.
34450143	6	45	theme	adsorption	925:934	arg1	capacity					936:943	the maximum adsorption capacity	913:943	the maximum adsorption capacity of CVD, MBD, and MVD to the desired composite	913:989	Also, the maximum adsorption capacity of CVD, MBD, and MVD to the desired composite was determined as 32.84 mg.g-1, 31.81 mg.g-1, and 31.15 mg.g-1, respectively.
34450143	6	45	theme	adsorption	925:934	arg1	31.81 mg.g-1					1023:1034	31.81 mg.g-1	1023:1034	31.81 mg.g-1	1023:1034	Also, the maximum adsorption capacity of CVD, MBD, and MVD to the desired composite was determined as 32.84 mg.g-1, 31.81 mg.g-1, and 31.15 mg.g-1, respectively.
34450143	6	45	theme	adsorption	925:934	arg1	31.15 mg.g-1					1041:1052	31.15 mg.g-1	1041:1052	31.15 mg.g-1	1041:1052	Also, the maximum adsorption capacity of CVD, MBD, and MVD to the desired composite was determined as 32.84 mg.g-1, 31.81 mg.g-1, and 31.15 mg.g-1, respectively.
34450143	3	46	theme	CLT/Starch/CoFe2O4	613:630	arg1	powder					632:637	CLT/Starch/CoFe2O4 powder	613:637	CLT/Starch/CoFe2O4 powder	613:637	The BET analysis showed that CLT modification using starch and CoFe2O4 nanoparticles improved its specific surface and the amount of specific surface area for CLT, CoFe2O4, and CLT/Starch/CoFe2O4 powder was reported to be 18.82 m2.g-1, 151.4 m2.g-1, and 104.75 m2.g-1, respectively.
34450143	3	47	theme	specific	569:576	arg1	area					586:589	specific surface area	569:589	specific surface area for CLT, CoFe2O4, and CLT/Starch/CoFe2O4 powder	569:637	The BET analysis showed that CLT modification using starch and CoFe2O4 nanoparticles improved its specific surface and the amount of specific surface area for CLT, CoFe2O4, and CLT/Starch/CoFe2O4 powder was reported to be 18.82 m2.g-1, 151.4 m2.g-1, and 104.75 m2.g-1, respectively.
34450143	9	48	theme	ad	1279:1280	arg1	results					1307:1313	ad(de)sorption experiments' results	1279:1313	ad(de)sorption experiments' results	1279:1313	Finally, ad(de)sorption experiments' results showed that the synthesized nanocomposite adsorbent has an excellent ability to adsorb cationic dyes after several consecutive cycles.
34450143	2	49	theme	violet	371:376	arg1	MVD					383:385	MVD	383:385	MVD	383:385	The prepared magnetic composite powder was utilized for decontamination of methylene blue dye (MBD), methyl violet dye (MVD), and crystal violet dye (CVD) from water media.
34450143	2	49	theme	violet	371:376	arg1	dye					378:380	methyl violet dye	364:380	methyl violet dye (MVD)	364:386	The prepared magnetic composite powder was utilized for decontamination of methylene blue dye (MBD), methyl violet dye (MVD), and crystal violet dye (CVD) from water media.
34450143	6	50	theme	maximum	917:923	arg1	32.84 mg.g-1					1009:1020	32.84 mg.g-1	1009:1020	32.84 mg.g-1	1009:1020	Also, the maximum adsorption capacity of CVD, MBD, and MVD to the desired composite was determined as 32.84 mg.g-1, 31.81 mg.g-1, and 31.15 mg.g-1, respectively.
34450143	6	50	theme	maximum	917:923	arg1	capacity					936:943	the maximum adsorption capacity	913:943	the maximum adsorption capacity of CVD, MBD, and MVD to the desired composite	913:989	Also, the maximum adsorption capacity of CVD, MBD, and MVD to the desired composite was determined as 32.84 mg.g-1, 31.81 mg.g-1, and 31.15 mg.g-1, respectively.
34450143	6	50	theme	maximum	917:923	arg1	31.81 mg.g-1					1023:1034	31.81 mg.g-1	1023:1034	31.81 mg.g-1	1023:1034	Also, the maximum adsorption capacity of CVD, MBD, and MVD to the desired composite was determined as 32.84 mg.g-1, 31.81 mg.g-1, and 31.15 mg.g-1, respectively.
34450143	6	50	theme	maximum	917:923	arg1	31.15 mg.g-1					1041:1052	31.15 mg.g-1	1041:1052	31.15 mg.g-1	1041:1052	Also, the maximum adsorption capacity of CVD, MBD, and MVD to the desired composite was determined as 32.84 mg.g-1, 31.81 mg.g-1, and 31.15 mg.g-1, respectively.
34450143	3	51	theme	surface	578:584	arg1	area					586:589	specific surface area	569:589	specific surface area for CLT, CoFe2O4, and CLT/Starch/CoFe2O4 powder	569:637	The BET analysis showed that CLT modification using starch and CoFe2O4 nanoparticles improved its specific surface and the amount of specific surface area for CLT, CoFe2O4, and CLT/Starch/CoFe2O4 powder was reported to be 18.82 m2.g-1, 151.4 m2.g-1, and 104.75 m2.g-1, respectively.
34450143	2	52	theme	methyl	364:369	arg1	MVD					383:385	MVD	383:385	MVD	383:385	The prepared magnetic composite powder was utilized for decontamination of methylene blue dye (MBD), methyl violet dye (MVD), and crystal violet dye (CVD) from water media.
34450143	2	52	theme	methyl	364:369	arg1	dye					378:380	methyl violet dye	364:380	methyl violet dye (MVD)	364:386	The prepared magnetic composite powder was utilized for decontamination of methylene blue dye (MBD), methyl violet dye (MVD), and crystal violet dye (CVD) from water media.
34450143	4	53	contain	had	757:759	arg2	role					769:772	a vital role	761:772	a vital role	761:772	Experimental results showed that pH 9 had a vital role in the adsorption process of all three types.
34450143	4	53	contain	had	757:759	arg1	pH 9					752:755	pH 9	752:755	pH 9	752:755	Experimental results showed that pH 9 had a vital role in the adsorption process of all three types.
34450143	0	54	from	water	129:133	arg1	dyes					119:122	cationic dyes	110:122	cationic dyes from water and wastewater	110:148	Preparation of clinoptilolite/starch/CoFe2O4 magnetic nanocomposite powder and its elimination properties for cationic dyes from water and wastewater.
34450143	2	55	theme	prepared	267:274	arg1	powder					295:300	The prepared magnetic composite powder	263:300	The prepared magnetic composite powder	263:300	The prepared magnetic composite powder was utilized for decontamination of methylene blue dye (MBD), methyl violet dye (MVD), and crystal violet dye (CVD) from water media.
34450143	1	56	theme	new	153:155	arg1	/Starch/CoFe2O4					200:214	A new magnetic nanocomposite clinoptilolite (CLT)/Starch/CoFe2O4	151:214	A new magnetic nanocomposite clinoptilolite (CLT)/Starch/CoFe2O4	151:214	A new magnetic nanocomposite clinoptilolite (CLT)/Starch/CoFe2O4 was synthesized using co-precipitation method.
34450143	7	57	theme	process	1114:1120	arg1	data					1094:1097	the kinetic data	1082:1097	the kinetic data of the removal process	1082:1120	In addition, the kinetic data of the removal process followed a pseudo-first order (PFO) kinetic model.
34450143	9	58	theme	synthesized	1331:1341	arg1	nanocomposite					1343:1355	the synthesized nanocomposite adsorbent	1327:1365	the synthesized nanocomposite adsorbent	1327:1365	Finally, ad(de)sorption experiments' results showed that the synthesized nanocomposite adsorbent has an excellent ability to adsorb cationic dyes after several consecutive cycles.
34450143	0	59	theme	properties	95:104	arg1	Preparation					0:10	Preparation	0:10	Preparation of clinoptilolite/starch/CoFe2O4 magnetic nanocomposite powder and its elimination properties for cationic dyes from water and wastewater.	0:149	Preparation of clinoptilolite/starch/CoFe2O4 magnetic nanocomposite powder and its elimination properties for cationic dyes from water and wastewater.
34450143	3	60	theme	BET	440:442	arg1	analysis					444:451	The BET analysis	436:451	The BET analysis	436:451	The BET analysis showed that CLT modification using starch and CoFe2O4 nanoparticles improved its specific surface and the amount of specific surface area for CLT, CoFe2O4, and CLT/Starch/CoFe2O4 powder was reported to be 18.82 m2.g-1, 151.4 m2.g-1, and 104.75 m2.g-1, respectively.
34450143	4	61	theme	adsorption	781:790	arg1	process					792:798	the adsorption process	777:798	the adsorption process of all three types	777:817	Experimental results showed that pH 9 had a vital role in the adsorption process of all three types.
34450143	1	62	theme	magnetic	157:164	arg1	CLT					196:198	CLT	196:198	CLT	196:198	A new magnetic nanocomposite clinoptilolite (CLT)/Starch/CoFe2O4 was synthesized using co-precipitation method.
34450143	1	62	theme	magnetic	157:164	arg1	clinoptilolite					180:193	magnetic nanocomposite clinoptilolite	157:193	A new magnetic nanocomposite clinoptilolite (CLT)/Starch/CoFe2O4	151:214	A new magnetic nanocomposite clinoptilolite (CLT)/Starch/CoFe2O4 was synthesized using co-precipitation method.
34450143	5	63	theme	isotherm	850:857	arg1	models					859:864	Langmuir and Redlich-Petersen isotherm models	820:864	Langmuir and Redlich-Petersen isotherm models	820:864	Langmuir and Redlich-Petersen isotherm models were well fitted with experimental data.
34450143	6	64	theme	desired	973:979	arg1	composite					981:989	the desired composite	969:989	the desired composite	969:989	Also, the maximum adsorption capacity of CVD, MBD, and MVD to the desired composite was determined as 32.84 mg.g-1, 31.81 mg.g-1, and 31.15 mg.g-1, respectively.
34450143	0	65	theme	elimination	83:93	arg1	properties					95:104	its elimination properties	79:104	its elimination properties	79:104	Preparation of clinoptilolite/starch/CoFe2O4 magnetic nanocomposite powder and its elimination properties for cationic dyes from water and wastewater.
34450143	8	66	theme	thermodynamic	1182:1194	arg1	parameters					1196:1205	Negative thermodynamic parameters	1173:1205	Negative thermodynamic parameters	1173:1205	Negative thermodynamic parameters were indicated that the process is spontaneous and exothermic.
34450143	2	67	theme	composite	285:293	arg1	powder					295:300	The prepared magnetic composite powder	263:300	The prepared magnetic composite powder	263:300	The prepared magnetic composite powder was utilized for decontamination of methylene blue dye (MBD), methyl violet dye (MVD), and crystal violet dye (CVD) from water media.
34450143	1	68	theme	nanocomposite	166:178	arg1	CLT					196:198	CLT	196:198	CLT	196:198	A new magnetic nanocomposite clinoptilolite (CLT)/Starch/CoFe2O4 was synthesized using co-precipitation method.
34450143	1	68	theme	nanocomposite	166:178	arg1	clinoptilolite					180:193	magnetic nanocomposite clinoptilolite	157:193	A new magnetic nanocomposite clinoptilolite (CLT)/Starch/CoFe2O4	151:214	A new magnetic nanocomposite clinoptilolite (CLT)/Starch/CoFe2O4 was synthesized using co-precipitation method.
34450143	0	69	from	wastewater	139:148	arg1	dyes					119:122	cationic dyes	110:122	cationic dyes from water and wastewater	110:148	Preparation of clinoptilolite/starch/CoFe2O4 magnetic nanocomposite powder and its elimination properties for cationic dyes from water and wastewater.
34757957	5	0	theme	PVA	737:739	arg1	incorporation					741:753	PVA incorporation	737:753	PVA incorporation	737:753	The positive effect of PVA incorporation was approved by analyzing BSM/PVA solutions through physiochemical assays such as electrical conductivity, viscosity and surface tension tests.
34757957	6	1	theme	samples	999:1005	arg1	stability					982:990	thermal stability	974:990	thermal stability of the samples	974:1005	According to the thermal analysis (TGA/DSC), incorporation of PVA enhanced thermal stability of the samples.
34757957	4	2	theme	desirable	668:676	arg1	structure					687:695	the most desirable scaffold structure	659:695	the most desirable scaffold structure for cell growth	659:711	It is found that the optimal bead-free ratio of 50/50 with a mean fiber diameter of ≈180 nm presents the most desirable scaffold structure for cell growth.
34757957	0	3	theme	mucilage/PVA	78:89	arg1	composites					91:100	balangu seed mucilage/PVA composites	65:100	balangu seed mucilage/PVA composites	65:100	Biocompatibility of electrospun cell culture scaffolds made from balangu seed mucilage/PVA composites.
34757957	1	4	theme	BSM	162:164	arg1	solutions					167:175	Balangu (Lallemantia royleana) seed mucilage (BSM) solutions	116:175	Balangu (Lallemantia royleana) seed mucilage (BSM) solutions combined with polyvinyl alcohol (PVA)	116:213	Synthesis of Balangu (Lallemantia royleana) seed mucilage (BSM) solutions combined with polyvinyl alcohol (PVA) was studied for the purpose of producing 3D electrospun cell culture scaffolds.
34757957	9	5	theme	MTT	1287:1289	arg1	assay					1291:1295	The MTT assay	1283:1295	The MTT assay	1283:1295	The MTT assay is employed to verify the biocompatibility of the scaffolds.
34757957	7	6	theme	major	1088:1092	arg1	interaction					1103:1113	no major chemical interaction	1085:1113	no major chemical interaction between BSM and PVA	1085:1133	Successful fabrication of the nanofibers is verified by FT-IR spectra, where no major chemical interaction between BSM and PVA is detected.
34757957	4	7	with	ratio	597:601	arg1	diameter					630:637	a mean fiber diameter	617:637	a mean fiber diameter of ≈180 nm	617:648	It is found that the optimal bead-free ratio of 50/50 with a mean fiber diameter of ≈180 nm presents the most desirable scaffold structure for cell growth.
34757957	5	8	theme	tension	884:890	arg1	tests					892:896	surface tension tests	876:896	surface tension tests	876:896	The positive effect of PVA incorporation was approved by analyzing BSM/PVA solutions through physiochemical assays such as electrical conductivity, viscosity and surface tension tests.
34757957	4	9	theme	optimal	579:585	arg1	ratio					597:601	the optimal bead-free ratio	575:601	the optimal bead-free ratio of 50/50 with a mean fiber diameter of ≈180 nm	575:648	It is found that the optimal bead-free ratio of 50/50 with a mean fiber diameter of ≈180 nm presents the most desirable scaffold structure for cell growth.
34757957	10	10	theme	incubation	1541:1550	arg1	h					1536:1536	72 h	1533:1536	72 h of incubation	1533:1550	The cell culture experiment using epithelial Vero cells shows the affinity of the cells to adhere to their nanofibrous substrate and grow to form continuous cell layers after 72 h of incubation.
34757957	7	11	theme	chemical	1094:1101	arg1	interaction					1103:1113	no major chemical interaction	1085:1113	no major chemical interaction between BSM and PVA	1085:1133	Successful fabrication of the nanofibers is verified by FT-IR spectra, where no major chemical interaction between BSM and PVA is detected.
34757957	10	12	theme	culture	1367:1373	arg1	experiment					1375:1384	The cell culture experiment	1358:1384	The cell culture experiment using epithelial Vero cells	1358:1412	The cell culture experiment using epithelial Vero cells shows the affinity of the cells to adhere to their nanofibrous substrate and grow to form continuous cell layers after 72 h of incubation.
34757957	3	13	theme	cell	538:541	arg1	proliferation					543:555	cell proliferation	538:555	cell proliferation	538:555	Different BSM/PVA ratios were fabricated to achieve the desired nanofibrous structure for cell proliferation.
34757957	9	14	theme	scaffolds	1347:1355	arg1	biocompatibility					1323:1338	the biocompatibility	1319:1338	the biocompatibility of the scaffolds	1319:1355	The MTT assay is employed to verify the biocompatibility of the scaffolds.
34757957	5	15	theme	incorporation	741:753	arg1	approved					759:766	approved	759:766	approved	759:766	The positive effect of PVA incorporation was approved by analyzing BSM/PVA solutions through physiochemical assays such as electrical conductivity, viscosity and surface tension tests.
34757957	5	15	theme	incorporation	741:753	arg1	effect					727:732	The positive effect	714:732	The positive effect of PVA incorporation	714:753	The positive effect of PVA incorporation was approved by analyzing BSM/PVA solutions through physiochemical assays such as electrical conductivity, viscosity and surface tension tests.
34757957	8	16	theme	scaffolds	1272:1280	arg1	structure					1251:1259	the nearly amorphous structure	1230:1259	the nearly amorphous structure of BSM/PVA scaffolds	1230:1280	The crystallinity of the electrospun nanofibers is investigated by XRD, revealing the nearly amorphous structure of BSM/PVA scaffolds.
34757957	3	17	theme	nanofibrous	512:522	arg1	structure					524:532	the desired nanofibrous structure	500:532	the desired nanofibrous structure for cell proliferation	500:555	Different BSM/PVA ratios were fabricated to achieve the desired nanofibrous structure for cell proliferation.
34757957	8	18	theme	electrospun	1173:1183	arg1	nanofibers					1185:1194	the electrospun nanofibers	1169:1194	the electrospun nanofibers	1169:1194	The crystallinity of the electrospun nanofibers is investigated by XRD, revealing the nearly amorphous structure of BSM/PVA scaffolds.
34757957	2	19	theme	PVA	372:374	arg1	integration					357:367	integration	357:367	integration of PVA	357:374	Production of pure BSM nanofibers proved to be difficult, yet integration of PVA contributed to a facile and successful formation of BSM/PVA nanofibers.
34757957	5	20	theme	positive	718:725	arg1	approved					759:766	approved	759:766	approved	759:766	The positive effect of PVA incorporation was approved by analyzing BSM/PVA solutions through physiochemical assays such as electrical conductivity, viscosity and surface tension tests.
34757957	5	20	theme	positive	718:725	arg1	effect					727:732	The positive effect	714:732	The positive effect of PVA incorporation	714:753	The positive effect of PVA incorporation was approved by analyzing BSM/PVA solutions through physiochemical assays such as electrical conductivity, viscosity and surface tension tests.
34757957	4	21	theme	bead-free	587:595	arg1	ratio					597:601	the optimal bead-free ratio	575:601	the optimal bead-free ratio of 50/50 with a mean fiber diameter of ≈180 nm	575:648	It is found that the optimal bead-free ratio of 50/50 with a mean fiber diameter of ≈180 nm presents the most desirable scaffold structure for cell growth.
34757957	1	22	theme	solutions	167:175	arg1	Synthesis					103:111	Synthesis	103:111	Synthesis of Balangu (Lallemantia royleana) seed mucilage (BSM) solutions combined with polyvinyl alcohol (PVA)	103:213	Synthesis of Balangu (Lallemantia royleana) seed mucilage (BSM) solutions combined with polyvinyl alcohol (PVA) was studied for the purpose of producing 3D electrospun cell culture scaffolds.
34757957	5	23	theme	BSM/PVA	781:787	arg1	solutions					789:797	BSM/PVA solutions	781:797	BSM/PVA solutions	781:797	The positive effect of PVA incorporation was approved by analyzing BSM/PVA solutions through physiochemical assays such as electrical conductivity, viscosity and surface tension tests.
34757957	3	24	theme	desired	504:510	arg1	structure					524:532	the desired nanofibrous structure	500:532	the desired nanofibrous structure for cell proliferation	500:555	Different BSM/PVA ratios were fabricated to achieve the desired nanofibrous structure for cell proliferation.
34757957	6	25	theme	PVA	961:963	arg1	incorporation					944:956	incorporation	944:956	incorporation of PVA	944:963	According to the thermal analysis (TGA/DSC), incorporation of PVA enhanced thermal stability of the samples.
34757957	7	26	theme	nanofibers	1038:1047	arg1	fabrication					1019:1029	Successful fabrication	1008:1029	Successful fabrication of the nanofibers	1008:1047	Successful fabrication of the nanofibers is verified by FT-IR spectra, where no major chemical interaction between BSM and PVA is detected.
34757957	4	27	theme	nm	647:648	arg1	diameter					630:637	a mean fiber diameter	617:637	a mean fiber diameter of ≈180 nm	617:648	It is found that the optimal bead-free ratio of 50/50 with a mean fiber diameter of ≈180 nm presents the most desirable scaffold structure for cell growth.
34757957	1	28	theme	3D	256:257	arg1	scaffolds					284:292	3D electrospun cell culture scaffolds	256:292	3D electrospun cell culture scaffolds	256:292	Synthesis of Balangu (Lallemantia royleana) seed mucilage (BSM) solutions combined with polyvinyl alcohol (PVA) was studied for the purpose of producing 3D electrospun cell culture scaffolds.
34757957	10	29	theme	cells	1440:1444	arg1	affinity					1424:1431	the affinity	1420:1431	the affinity of the cells to adhere to their nanofibrous substrate and grow to form continuous cell layers after 72 h of incubation	1420:1550	The cell culture experiment using epithelial Vero cells shows the affinity of the cells to adhere to their nanofibrous substrate and grow to form continuous cell layers after 72 h of incubation.
34757957	10	30	theme	Vero	1403:1406	arg1	cells					1408:1412	epithelial Vero cells	1392:1412	epithelial Vero cells	1392:1412	The cell culture experiment using epithelial Vero cells shows the affinity of the cells to adhere to their nanofibrous substrate and grow to form continuous cell layers after 72 h of incubation.
34757957	8	31	theme	amorphous	1241:1249	arg1	structure					1251:1259	the nearly amorphous structure	1230:1259	the nearly amorphous structure of BSM/PVA scaffolds	1230:1280	The crystallinity of the electrospun nanofibers is investigated by XRD, revealing the nearly amorphous structure of BSM/PVA scaffolds.
34757957	0	32	theme	cell	32:35	arg1	culture					37:43	electrospun cell culture	20:43	electrospun cell culture	20:43	Biocompatibility of electrospun cell culture scaffolds made from balangu seed mucilage/PVA composites.
34757957	1	33	theme	electrospun	259:269	arg1	scaffolds					284:292	3D electrospun cell culture scaffolds	256:292	3D electrospun cell culture scaffolds	256:292	Synthesis of Balangu (Lallemantia royleana) seed mucilage (BSM) solutions combined with polyvinyl alcohol (PVA) was studied for the purpose of producing 3D electrospun cell culture scaffolds.
34757957	8	34	theme	nanofibers	1185:1194	arg1	crystallinity					1152:1164	The crystallinity	1148:1164	The crystallinity of the electrospun nanofibers	1148:1194	The crystallinity of the electrospun nanofibers is investigated by XRD, revealing the nearly amorphous structure of BSM/PVA scaffolds.
34757957	1	35	theme	polyvinyl	191:199	arg1	alcohol					201:207	polyvinyl alcohol	191:207	polyvinyl alcohol (PVA)	191:213	Synthesis of Balangu (Lallemantia royleana) seed mucilage (BSM) solutions combined with polyvinyl alcohol (PVA) was studied for the purpose of producing 3D electrospun cell culture scaffolds.
34757957	1	35	theme	polyvinyl	191:199	arg1	PVA					210:212	PVA	210:212	PVA	210:212	Synthesis of Balangu (Lallemantia royleana) seed mucilage (BSM) solutions combined with polyvinyl alcohol (PVA) was studied for the purpose of producing 3D electrospun cell culture scaffolds.
34757957	0	36	theme	electrospun	20:30	arg1	culture					37:43	electrospun cell culture	20:43	electrospun cell culture	20:43	Biocompatibility of electrospun cell culture scaffolds made from balangu seed mucilage/PVA composites.
34757957	1	37	theme	cell	271:274	arg1	scaffolds					284:292	3D electrospun cell culture scaffolds	256:292	3D electrospun cell culture scaffolds	256:292	Synthesis of Balangu (Lallemantia royleana) seed mucilage (BSM) solutions combined with polyvinyl alcohol (PVA) was studied for the purpose of producing 3D electrospun cell culture scaffolds.
34757957	7	38	theme	FT-IR	1064:1068	arg1	spectra					1070:1076	FT-IR spectra	1064:1076	FT-IR spectra	1064:1076	Successful fabrication of the nanofibers is verified by FT-IR spectra, where no major chemical interaction between BSM and PVA is detected.
34757957	1	39	theme	Balangu	116:122	arg1	solutions					167:175	Balangu (Lallemantia royleana) seed mucilage (BSM) solutions	116:175	Balangu (Lallemantia royleana) seed mucilage (BSM) solutions combined with polyvinyl alcohol (PVA)	116:213	Synthesis of Balangu (Lallemantia royleana) seed mucilage (BSM) solutions combined with polyvinyl alcohol (PVA) was studied for the purpose of producing 3D electrospun cell culture scaffolds.
34757957	3	40	theme	Different	448:456	arg1	ratios					466:471	Different BSM/PVA ratios	448:471	Different BSM/PVA ratios	448:471	Different BSM/PVA ratios were fabricated to achieve the desired nanofibrous structure for cell proliferation.
34757957	10	41	theme	cell	1515:1518	arg1	layers					1520:1525	continuous cell layers	1504:1525	continuous cell layers	1504:1525	The cell culture experiment using epithelial Vero cells shows the affinity of the cells to adhere to their nanofibrous substrate and grow to form continuous cell layers after 72 h of incubation.
34757957	1	42	theme	culture	276:282	arg1	scaffolds					284:292	3D electrospun cell culture scaffolds	256:292	3D electrospun cell culture scaffolds	256:292	Synthesis of Balangu (Lallemantia royleana) seed mucilage (BSM) solutions combined with polyvinyl alcohol (PVA) was studied for the purpose of producing 3D electrospun cell culture scaffolds.
34757957	10	43	theme	nanofibrous	1465:1475	arg1	substrate					1477:1485	their nanofibrous substrate	1459:1485	their nanofibrous substrate	1459:1485	The cell culture experiment using epithelial Vero cells shows the affinity of the cells to adhere to their nanofibrous substrate and grow to form continuous cell layers after 72 h of incubation.
34757957	8	44	theme	BSM/PVA	1264:1270	arg1	scaffolds					1272:1280	BSM/PVA scaffolds	1264:1280	BSM/PVA scaffolds	1264:1280	The crystallinity of the electrospun nanofibers is investigated by XRD, revealing the nearly amorphous structure of BSM/PVA scaffolds.
34757957	4	45	theme	fiber	624:628	arg1	diameter					630:637	a mean fiber diameter	617:637	a mean fiber diameter of ≈180 nm	617:648	It is found that the optimal bead-free ratio of 50/50 with a mean fiber diameter of ≈180 nm presents the most desirable scaffold structure for cell growth.
34757957	0	46	theme	culture	37:43	arg1	Biocompatibility					0:15	Biocompatibility	0:15	Biocompatibility of electrospun cell culture	0:43	Biocompatibility of electrospun cell culture scaffolds made from balangu seed mucilage/PVA composites.
34757957	2	47	theme	successful	404:413	arg1	formation					415:423	a facile and successful formation	391:423	a facile and successful formation of BSM/PVA nanofibers	391:445	Production of pure BSM nanofibers proved to be difficult, yet integration of PVA contributed to a facile and successful formation of BSM/PVA nanofibers.
34757957	5	48	theme	electrical	837:846	arg1	conductivity					848:859	electrical conductivity	837:859	electrical conductivity	837:859	The positive effect of PVA incorporation was approved by analyzing BSM/PVA solutions through physiochemical assays such as electrical conductivity, viscosity and surface tension tests.
34757957	4	49	theme	mean	619:622	arg1	diameter					630:637	a mean fiber diameter	617:637	a mean fiber diameter of ≈180 nm	617:648	It is found that the optimal bead-free ratio of 50/50 with a mean fiber diameter of ≈180 nm presents the most desirable scaffold structure for cell growth.
34757957	2	50	theme	nanofibers	436:445	arg1	formation					415:423	a facile and successful formation	391:423	a facile and successful formation of BSM/PVA nanofibers	391:445	Production of pure BSM nanofibers proved to be difficult, yet integration of PVA contributed to a facile and successful formation of BSM/PVA nanofibers.
34757957	5	51	theme	surface	876:882	arg1	tests					892:896	surface tension tests	876:896	surface tension tests	876:896	The positive effect of PVA incorporation was approved by analyzing BSM/PVA solutions through physiochemical assays such as electrical conductivity, viscosity and surface tension tests.
34757957	10	52	theme	continuous	1504:1513	arg1	layers					1520:1525	continuous cell layers	1504:1525	continuous cell layers	1504:1525	The cell culture experiment using epithelial Vero cells shows the affinity of the cells to adhere to their nanofibrous substrate and grow to form continuous cell layers after 72 h of incubation.
34757957	0	53	dep	scaffolds	45:53	arg1	made					55:58	made	55:58	scaffolds made from balangu seed mucilage/PVA composites	45:100	Biocompatibility of electrospun cell culture scaffolds made from balangu seed mucilage/PVA composites.
34757957	7	54	theme	Successful	1008:1017	arg1	fabrication					1019:1029	Successful fabrication	1008:1029	Successful fabrication of the nanofibers	1008:1047	Successful fabrication of the nanofibers is verified by FT-IR spectra, where no major chemical interaction between BSM and PVA is detected.
34757957	2	55	theme	BSM/PVA	428:434	arg1	nanofibers					436:445	BSM/PVA nanofibers	428:445	BSM/PVA nanofibers	428:445	Production of pure BSM nanofibers proved to be difficult, yet integration of PVA contributed to a facile and successful formation of BSM/PVA nanofibers.
34757957	3	56	theme	BSM/PVA	458:464	arg1	ratios					466:471	Different BSM/PVA ratios	448:471	Different BSM/PVA ratios	448:471	Different BSM/PVA ratios were fabricated to achieve the desired nanofibrous structure for cell proliferation.
34757957	1	57	theme	Lallemantia	125:135	arg1	solutions					167:175	Balangu (Lallemantia royleana) seed mucilage (BSM) solutions	116:175	Balangu (Lallemantia royleana) seed mucilage (BSM) solutions combined with polyvinyl alcohol (PVA)	116:213	Synthesis of Balangu (Lallemantia royleana) seed mucilage (BSM) solutions combined with polyvinyl alcohol (PVA) was studied for the purpose of producing 3D electrospun cell culture scaffolds.
34757957	10	58	theme	cell	1362:1365	arg1	experiment					1375:1384	The cell culture experiment	1358:1384	The cell culture experiment using epithelial Vero cells	1358:1412	The cell culture experiment using epithelial Vero cells shows the affinity of the cells to adhere to their nanofibrous substrate and grow to form continuous cell layers after 72 h of incubation.
34757957	10	59	theme	epithelial	1392:1401	arg1	cells					1408:1412	epithelial Vero cells	1392:1412	epithelial Vero cells	1392:1412	The cell culture experiment using epithelial Vero cells shows the affinity of the cells to adhere to their nanofibrous substrate and grow to form continuous cell layers after 72 h of incubation.
34757957	1	60	theme	royleana	137:144	arg1	solutions					167:175	Balangu (Lallemantia royleana) seed mucilage (BSM) solutions	116:175	Balangu (Lallemantia royleana) seed mucilage (BSM) solutions combined with polyvinyl alcohol (PVA)	116:213	Synthesis of Balangu (Lallemantia royleana) seed mucilage (BSM) solutions combined with polyvinyl alcohol (PVA) was studied for the purpose of producing 3D electrospun cell culture scaffolds.
34757957	6	61	theme	thermal	974:980	arg1	stability					982:990	thermal stability	974:990	thermal stability of the samples	974:1005	According to the thermal analysis (TGA/DSC), incorporation of PVA enhanced thermal stability of the samples.
34757957	4	62	theme	cell	701:704	arg1	growth					706:711	cell growth	701:711	cell growth	701:711	It is found that the optimal bead-free ratio of 50/50 with a mean fiber diameter of ≈180 nm presents the most desirable scaffold structure for cell growth.
34757957	2	63	theme	pure	309:312	arg1	nanofibers					318:327	pure BSM nanofibers	309:327	pure BSM nanofibers	309:327	Production of pure BSM nanofibers proved to be difficult, yet integration of PVA contributed to a facile and successful formation of BSM/PVA nanofibers.
34757957	2	64	theme	BSM	314:316	arg1	nanofibers					318:327	pure BSM nanofibers	309:327	pure BSM nanofibers	309:327	Production of pure BSM nanofibers proved to be difficult, yet integration of PVA contributed to a facile and successful formation of BSM/PVA nanofibers.
34757957	0	65	theme	seed	73:76	arg1	composites					91:100	balangu seed mucilage/PVA composites	65:100	balangu seed mucilage/PVA composites	65:100	Biocompatibility of electrospun cell culture scaffolds made from balangu seed mucilage/PVA composites.
34757957	2	66	theme	nanofibers	318:327	arg1	Production					295:304	Production	295:304	Production of pure BSM nanofibers	295:327	Production of pure BSM nanofibers proved to be difficult, yet integration of PVA contributed to a facile and successful formation of BSM/PVA nanofibers.
34757957	2	67	theme	facile	393:398	arg1	formation					415:423	a facile and successful formation	391:423	a facile and successful formation of BSM/PVA nanofibers	391:445	Production of pure BSM nanofibers proved to be difficult, yet integration of PVA contributed to a facile and successful formation of BSM/PVA nanofibers.
34757957	1	68	theme	seed	147:150	arg1	solutions					167:175	Balangu (Lallemantia royleana) seed mucilage (BSM) solutions	116:175	Balangu (Lallemantia royleana) seed mucilage (BSM) solutions combined with polyvinyl alcohol (PVA)	116:213	Synthesis of Balangu (Lallemantia royleana) seed mucilage (BSM) solutions combined with polyvinyl alcohol (PVA) was studied for the purpose of producing 3D electrospun cell culture scaffolds.
34757957	4	69	theme	50/50	606:610	arg1	ratio					597:601	the optimal bead-free ratio	575:601	the optimal bead-free ratio of 50/50 with a mean fiber diameter of ≈180 nm	575:648	It is found that the optimal bead-free ratio of 50/50 with a mean fiber diameter of ≈180 nm presents the most desirable scaffold structure for cell growth.
34757957	0	70	theme	balangu	65:71	arg1	composites					91:100	balangu seed mucilage/PVA composites	65:100	balangu seed mucilage/PVA composites	65:100	Biocompatibility of electrospun cell culture scaffolds made from balangu seed mucilage/PVA composites.
34757957	6	71	theme	thermal	916:922	arg1	TGA/DSC					934:940	TGA/DSC	934:940	TGA/DSC	934:940	According to the thermal analysis (TGA/DSC), incorporation of PVA enhanced thermal stability of the samples.
34757957	6	71	theme	thermal	916:922	arg1	analysis					924:931	the thermal analysis	912:931	the thermal analysis (TGA/DSC)	912:941	According to the thermal analysis (TGA/DSC), incorporation of PVA enhanced thermal stability of the samples.
34757957	5	72	theme	physiochemical	807:820	arg1	tests					892:896	surface tension tests	876:896	surface tension tests	876:896	The positive effect of PVA incorporation was approved by analyzing BSM/PVA solutions through physiochemical assays such as electrical conductivity, viscosity and surface tension tests.
34757957	5	72	theme	physiochemical	807:820	arg1	assays					822:827	physiochemical assays	807:827	physiochemical assays such as electrical conductivity, viscosity and surface tension tests	807:896	The positive effect of PVA incorporation was approved by analyzing BSM/PVA solutions through physiochemical assays such as electrical conductivity, viscosity and surface tension tests.
34757957	5	72	theme	physiochemical	807:820	arg1	viscosity					862:870	viscosity	862:870	viscosity	862:870	The positive effect of PVA incorporation was approved by analyzing BSM/PVA solutions through physiochemical assays such as electrical conductivity, viscosity and surface tension tests.
34757957	5	72	theme	physiochemical	807:820	arg1	conductivity					848:859	electrical conductivity	837:859	electrical conductivity	837:859	The positive effect of PVA incorporation was approved by analyzing BSM/PVA solutions through physiochemical assays such as electrical conductivity, viscosity and surface tension tests.
34757957	1	73	theme	mucilage	152:159	arg1	solutions					167:175	Balangu (Lallemantia royleana) seed mucilage (BSM) solutions	116:175	Balangu (Lallemantia royleana) seed mucilage (BSM) solutions combined with polyvinyl alcohol (PVA)	116:213	Synthesis of Balangu (Lallemantia royleana) seed mucilage (BSM) solutions combined with polyvinyl alcohol (PVA) was studied for the purpose of producing 3D electrospun cell culture scaffolds.
34757957	4	74	theme	scaffold	678:685	arg1	structure					687:695	the most desirable scaffold structure	659:695	the most desirable scaffold structure for cell growth	659:711	It is found that the optimal bead-free ratio of 50/50 with a mean fiber diameter of ≈180 nm presents the most desirable scaffold structure for cell growth.
31982527	8	0	theme	vaginal	1253:1259	arg1	infection					1261:1269	vaginal infection	1253:1269	vaginal infection of C.albicans	1253:1283	Our results suggest salecan might be a potential therapeutic agent for vaginal infection of C.albicans.
31982527	6	1	theme	vaginal	842:848	arg1	composition					870:880	vaginal microbial community composition	842:880	vaginal microbial community composition	842:880	The analysis for vaginal microbial community composition at different taxa levels revealed that the bacterial flora composition in the vagina of the salecan-treated mice was similar to that of the uninfected mice, and distinguished from the infected mice.
31982527	1	2	theme	common	134:139	arg1	infections					141:150	the most common infections	125:150	the most common infections in women	125:159	Vulvovaginal candidiasis (VVC) is one of the most common infections in women.
31982527	6	3	theme	microbial	850:858	arg1	composition					870:880	vaginal microbial community composition	842:880	vaginal microbial community composition	842:880	The analysis for vaginal microbial community composition at different taxa levels revealed that the bacterial flora composition in the vagina of the salecan-treated mice was similar to that of the uninfected mice, and distinguished from the infected mice.
31982527	1	4	theme	infections	141:150	arg1	one					118:120	one	118:120	one	118:120	Vulvovaginal candidiasis (VVC) is one of the most common infections in women.
31982527	1	4	theme	infections	141:150	arg1	infections					141:150	the most common infections	125:150	the most common infections in women	125:159	Vulvovaginal candidiasis (VVC) is one of the most common infections in women.
31982527	4	5	theme	infected	520:527	arg1	mice					529:532	infected mice	520:532	infected mice given saline	520:545	We found that mice that received salecan (0.5 mg per mouse) after infection had 85% fewer CFU than infected mice given saline.
31982527	6	6	theme	uninfected	1022:1031	arg1	mice					1033:1036	the uninfected mice	1018:1036	the uninfected mice	1018:1036	The analysis for vaginal microbial community composition at different taxa levels revealed that the bacterial flora composition in the vagina of the salecan-treated mice was similar to that of the uninfected mice, and distinguished from the infected mice.
31982527	8	7	dep	potential	1221:1229	arg1	therapeutic					1231:1241	therapeutic	1231:1241	therapeutic	1231:1241	Our results suggest salecan might be a potential therapeutic agent for vaginal infection of C.albicans.
31982527	1	8	from	infections	141:150	arg1	women					155:159	women	155:159	women	155:159	Vulvovaginal candidiasis (VVC) is one of the most common infections in women.
31982527	4	9	contain	had	497:499	arg2	CFU					511:513	85% fewer CFU	501:513	85% fewer CFU	501:513	We found that mice that received salecan (0.5 mg per mouse) after infection had 85% fewer CFU than infected mice given saline.
31982527	4	9	contain	had	497:499	arg1	mice					435:438	mice	435:438	mice that received salecan (0.5 mg per mouse) after infection	435:495	We found that mice that received salecan (0.5 mg per mouse) after infection had 85% fewer CFU than infected mice given saline.
31982527	6	10	theme	bacterial	925:933	arg1	composition					941:951	the bacterial flora composition	921:951	the bacterial flora composition in the vagina of the salecan-treated mice	921:993	The analysis for vaginal microbial community composition at different taxa levels revealed that the bacterial flora composition in the vagina of the salecan-treated mice was similar to that of the uninfected mice, and distinguished from the infected mice.
31982527	5	11	theme	decreased	669:677	arg1	levels					684:689	decreased mRNA levels	669:689	decreased mRNA levels of cytokines IL23, IL22, IL17a, and IL17f, anti-candidal genes S100a8 and S100a9 and C.albicans pattern recognition receptor Dectin1	669:822	Compared with the C.albicans group, salecan reduced the migration of polymorphonuclear neutrophils (PMNs) in the vagina, decreased mRNA levels of cytokines IL23, IL22, IL17a, and IL17f, anti-candidal genes S100a8 and S100a9 and C.albicans pattern recognition receptor Dectin1.
31982527	0	12	theme	Soluble	0:6	arg1	salecan					20:26	Soluble beta-glucan salecan	0:26	Soluble beta-glucan salecan	0:26	Soluble beta-glucan salecan improves vaginal infection of Candida albicans in mice.
31982527	5	13	theme	mRNA	679:682	arg1	levels					684:689	decreased mRNA levels	669:689	decreased mRNA levels of cytokines IL23, IL22, IL17a, and IL17f, anti-candidal genes S100a8 and S100a9 and C.albicans pattern recognition receptor Dectin1	669:822	Compared with the C.albicans group, salecan reduced the migration of polymorphonuclear neutrophils (PMNs) in the vagina, decreased mRNA levels of cytokines IL23, IL22, IL17a, and IL17f, anti-candidal genes S100a8 and S100a9 and C.albicans pattern recognition receptor Dectin1.
31982527	5	14	theme	C.albicans	566:575	arg1	group					577:581	the C.albicans group	562:581	the C.albicans group	562:581	Compared with the C.albicans group, salecan reduced the migration of polymorphonuclear neutrophils (PMNs) in the vagina, decreased mRNA levels of cytokines IL23, IL22, IL17a, and IL17f, anti-candidal genes S100a8 and S100a9 and C.albicans pattern recognition receptor Dectin1.
31982527	2	15	theme	salecan	244:250	arg1	ability					208:214	the ability	204:214	the ability of a water-soluble β-glucan salecan to protect against Candida albicans (C. albicans) vaginal infection	204:318	The purpose of this study was to evaluate the ability of a water-soluble β-glucan salecan to protect against Candida albicans (C. albicans) vaginal infection.
31982527	7	16	theme	treated	1118:1124	arg1	group					1126:1130	the salecan treated group	1106:1130	the salecan treated group	1106:1130	The vaginal lavages from the salecan treated group had more Enterococcus and its metabolite lactate.
31982527	0	17	from	infection	45:53	arg1	mice					78:81	mice	78:81	mice	78:81	Soluble beta-glucan salecan improves vaginal infection of Candida albicans in mice.
31982527	2	18	theme	β-glucan	235:242	arg1	salecan					244:250	a water-soluble β-glucan salecan	219:250	a water-soluble β-glucan salecan	219:250	The purpose of this study was to evaluate the ability of a water-soluble β-glucan salecan to protect against Candida albicans (C. albicans) vaginal infection.
31982527	5	19	theme	C.albicans	776:785	arg1	cytokines					694:702	cytokines IL23, IL22, IL17a, and IL17f	694:731	cytokines IL23, IL22, IL17a, and IL17f	694:731	Compared with the C.albicans group, salecan reduced the migration of polymorphonuclear neutrophils (PMNs) in the vagina, decreased mRNA levels of cytokines IL23, IL22, IL17a, and IL17f, anti-candidal genes S100a8 and S100a9 and C.albicans pattern recognition receptor Dectin1.
31982527	5	19	theme	C.albicans	776:785	arg1	Dectin1					816:822	C.albicans pattern recognition receptor Dectin1	776:822	C.albicans pattern recognition receptor Dectin1	776:822	Compared with the C.albicans group, salecan reduced the migration of polymorphonuclear neutrophils (PMNs) in the vagina, decreased mRNA levels of cytokines IL23, IL22, IL17a, and IL17f, anti-candidal genes S100a8 and S100a9 and C.albicans pattern recognition receptor Dectin1.
31982527	0	20	theme	beta-glucan	8:18	arg1	salecan					20:26	Soluble beta-glucan salecan	0:26	Soluble beta-glucan salecan	0:26	Soluble beta-glucan salecan improves vaginal infection of Candida albicans in mice.
31982527	6	21	from	composition	941:951	arg1	vagina					960:965	the vagina	956:965	the vagina of the salecan-treated mice	956:993	The analysis for vaginal microbial community composition at different taxa levels revealed that the bacterial flora composition in the vagina of the salecan-treated mice was similar to that of the uninfected mice, and distinguished from the infected mice.
31982527	1	22	theme	Vulvovaginal	84:95	arg1	candidiasis					97:107	Vulvovaginal candidiasis	84:107	Vulvovaginal candidiasis (VVC)	84:113	Vulvovaginal candidiasis (VVC) is one of the most common infections in women.
31982527	1	22	theme	Vulvovaginal	84:95	arg1	VVC					110:112	VVC	110:112	VVC	110:112	Vulvovaginal candidiasis (VVC) is one of the most common infections in women.
31982527	5	23	theme	pattern	787:793	arg1	cytokines					694:702	cytokines IL23, IL22, IL17a, and IL17f	694:731	cytokines IL23, IL22, IL17a, and IL17f	694:731	Compared with the C.albicans group, salecan reduced the migration of polymorphonuclear neutrophils (PMNs) in the vagina, decreased mRNA levels of cytokines IL23, IL22, IL17a, and IL17f, anti-candidal genes S100a8 and S100a9 and C.albicans pattern recognition receptor Dectin1.
31982527	5	23	theme	pattern	787:793	arg1	Dectin1					816:822	C.albicans pattern recognition receptor Dectin1	776:822	C.albicans pattern recognition receptor Dectin1	776:822	Compared with the C.albicans group, salecan reduced the migration of polymorphonuclear neutrophils (PMNs) in the vagina, decreased mRNA levels of cytokines IL23, IL22, IL17a, and IL17f, anti-candidal genes S100a8 and S100a9 and C.albicans pattern recognition receptor Dectin1.
31982527	3	24	theme	intravaginal	351:362	arg1	inoculation					364:374	intravaginal inoculation	351:374	intravaginal inoculation of yeast blastoconidia in pseudoestrus mice	351:418	The model was reproduced with intravaginal inoculation of yeast blastoconidia in pseudoestrus mice.
31982527	4	25	theme	fewer	505:509	arg1	CFU					511:513	85% fewer CFU	501:513	85% fewer CFU	501:513	We found that mice that received salecan (0.5 mg per mouse) after infection had 85% fewer CFU than infected mice given saline.
31982527	3	26	theme	pseudoestrus	402:413	arg1	mice					415:418	pseudoestrus mice	402:418	pseudoestrus mice	402:418	The model was reproduced with intravaginal inoculation of yeast blastoconidia in pseudoestrus mice.
31982527	7	27	theme	vaginal	1085:1091	arg1	lavages					1093:1099	The vaginal lavages	1081:1099	The vaginal lavages from the salecan treated group	1081:1130	The vaginal lavages from the salecan treated group had more Enterococcus and its metabolite lactate.
31982527	4	28	dep	salecan	454:460	arg1	0.5 mg					463:468	0.5 mg	463:468	0.5 mg	463:468	We found that mice that received salecan (0.5 mg per mouse) after infection had 85% fewer CFU than infected mice given saline.
31982527	6	29	from	levels	900:905	arg1	analysis					829:836	The analysis	825:836	The analysis for vaginal microbial community composition at different taxa levels	825:905	The analysis for vaginal microbial community composition at different taxa levels revealed that the bacterial flora composition in the vagina of the salecan-treated mice was similar to that of the uninfected mice, and distinguished from the infected mice.
31982527	2	30	theme	Candida	271:277	arg1	albicans					279:286	Candida albicans	271:286	Candida albicans	271:286	The purpose of this study was to evaluate the ability of a water-soluble β-glucan salecan to protect against Candida albicans (C. albicans) vaginal infection.
31982527	5	31	theme	genes	748:752	arg1	cytokines					694:702	cytokines IL23, IL22, IL17a, and IL17f	694:731	cytokines IL23, IL22, IL17a, and IL17f	694:731	Compared with the C.albicans group, salecan reduced the migration of polymorphonuclear neutrophils (PMNs) in the vagina, decreased mRNA levels of cytokines IL23, IL22, IL17a, and IL17f, anti-candidal genes S100a8 and S100a9 and C.albicans pattern recognition receptor Dectin1.
31982527	5	31	theme	genes	748:752	arg1	S100a8					754:759	anti-candidal genes S100a8	734:759	anti-candidal genes S100a8	734:759	Compared with the C.albicans group, salecan reduced the migration of polymorphonuclear neutrophils (PMNs) in the vagina, decreased mRNA levels of cytokines IL23, IL22, IL17a, and IL17f, anti-candidal genes S100a8 and S100a9 and C.albicans pattern recognition receptor Dectin1.
31982527	5	32	theme	anti-candidal	734:746	arg1	cytokines					694:702	cytokines IL23, IL22, IL17a, and IL17f	694:731	cytokines IL23, IL22, IL17a, and IL17f	694:731	Compared with the C.albicans group, salecan reduced the migration of polymorphonuclear neutrophils (PMNs) in the vagina, decreased mRNA levels of cytokines IL23, IL22, IL17a, and IL17f, anti-candidal genes S100a8 and S100a9 and C.albicans pattern recognition receptor Dectin1.
31982527	5	32	theme	anti-candidal	734:746	arg1	S100a8					754:759	anti-candidal genes S100a8	734:759	anti-candidal genes S100a8	734:759	Compared with the C.albicans group, salecan reduced the migration of polymorphonuclear neutrophils (PMNs) in the vagina, decreased mRNA levels of cytokines IL23, IL22, IL17a, and IL17f, anti-candidal genes S100a8 and S100a9 and C.albicans pattern recognition receptor Dectin1.
31982527	8	33	dep	suggest	1194:1200	arg1	agent					1243:1247	a potential therapeutic agent	1219:1247	a potential therapeutic agent for vaginal infection of C.albicans	1219:1283	Our results suggest salecan might be a potential therapeutic agent for vaginal infection of C.albicans.
31982527	6	34	theme	infected	1066:1073	arg1	mice					1075:1078	the infected mice	1062:1078	the infected mice	1062:1078	The analysis for vaginal microbial community composition at different taxa levels revealed that the bacterial flora composition in the vagina of the salecan-treated mice was similar to that of the uninfected mice, and distinguished from the infected mice.
31982527	6	35	theme	mice	990:993	arg1	vagina					960:965	the vagina	956:965	the vagina of the salecan-treated mice	956:993	The analysis for vaginal microbial community composition at different taxa levels revealed that the bacterial flora composition in the vagina of the salecan-treated mice was similar to that of the uninfected mice, and distinguished from the infected mice.
31982527	3	36	theme	yeast	379:383	arg1	blastoconidia					385:397	yeast blastoconidia	379:397	yeast blastoconidia	379:397	The model was reproduced with intravaginal inoculation of yeast blastoconidia in pseudoestrus mice.
31982527	6	37	theme	taxa	895:898	arg1	levels					900:905	different taxa levels	885:905	different taxa levels	885:905	The analysis for vaginal microbial community composition at different taxa levels revealed that the bacterial flora composition in the vagina of the salecan-treated mice was similar to that of the uninfected mice, and distinguished from the infected mice.
31982527	7	38	theme	salecan	1110:1116	arg1	group					1126:1130	the salecan treated group	1106:1130	the salecan treated group	1106:1130	The vaginal lavages from the salecan treated group had more Enterococcus and its metabolite lactate.
31982527	5	39	theme	recognition	795:805	arg1	cytokines					694:702	cytokines IL23, IL22, IL17a, and IL17f	694:731	cytokines IL23, IL22, IL17a, and IL17f	694:731	Compared with the C.albicans group, salecan reduced the migration of polymorphonuclear neutrophils (PMNs) in the vagina, decreased mRNA levels of cytokines IL23, IL22, IL17a, and IL17f, anti-candidal genes S100a8 and S100a9 and C.albicans pattern recognition receptor Dectin1.
31982527	5	39	theme	recognition	795:805	arg1	Dectin1					816:822	C.albicans pattern recognition receptor Dectin1	776:822	C.albicans pattern recognition receptor Dectin1	776:822	Compared with the C.albicans group, salecan reduced the migration of polymorphonuclear neutrophils (PMNs) in the vagina, decreased mRNA levels of cytokines IL23, IL22, IL17a, and IL17f, anti-candidal genes S100a8 and S100a9 and C.albicans pattern recognition receptor Dectin1.
31982527	6	40	theme	salecan-treated	974:988	arg1	mice					990:993	the salecan-treated mice	970:993	the salecan-treated mice	970:993	The analysis for vaginal microbial community composition at different taxa levels revealed that the bacterial flora composition in the vagina of the salecan-treated mice was similar to that of the uninfected mice, and distinguished from the infected mice.
31982527	0	41	theme	vaginal	37:43	arg1	infection					45:53	vaginal infection	37:53	vaginal infection of Candida albicans in mice	37:81	Soluble beta-glucan salecan improves vaginal infection of Candida albicans in mice.
31982527	6	42	theme	different	885:893	arg1	levels					900:905	different taxa levels	885:905	different taxa levels	885:905	The analysis for vaginal microbial community composition at different taxa levels revealed that the bacterial flora composition in the vagina of the salecan-treated mice was similar to that of the uninfected mice, and distinguished from the infected mice.
31982527	3	43	theme	blastoconidia	385:397	arg1	inoculation					364:374	intravaginal inoculation	351:374	intravaginal inoculation of yeast blastoconidia in pseudoestrus mice	351:418	The model was reproduced with intravaginal inoculation of yeast blastoconidia in pseudoestrus mice.
31982527	6	44	theme	flora	935:939	arg1	composition					941:951	the bacterial flora composition	921:951	the bacterial flora composition in the vagina of the salecan-treated mice	921:993	The analysis for vaginal microbial community composition at different taxa levels revealed that the bacterial flora composition in the vagina of the salecan-treated mice was similar to that of the uninfected mice, and distinguished from the infected mice.
31982527	0	45	theme	Candida	58:64	arg1	albicans					66:73	Candida albicans	58:73	Candida albicans	58:73	Soluble beta-glucan salecan improves vaginal infection of Candida albicans in mice.
31982527	5	46	theme	polymorphonuclear	617:633	arg1	PMNs					648:651	PMNs	648:651	PMNs	648:651	Compared with the C.albicans group, salecan reduced the migration of polymorphonuclear neutrophils (PMNs) in the vagina, decreased mRNA levels of cytokines IL23, IL22, IL17a, and IL17f, anti-candidal genes S100a8 and S100a9 and C.albicans pattern recognition receptor Dectin1.
31982527	5	46	theme	polymorphonuclear	617:633	arg1	neutrophils					635:645	polymorphonuclear neutrophils	617:645	polymorphonuclear neutrophils (PMNs)	617:652	Compared with the C.albicans group, salecan reduced the migration of polymorphonuclear neutrophils (PMNs) in the vagina, decreased mRNA levels of cytokines IL23, IL22, IL17a, and IL17f, anti-candidal genes S100a8 and S100a9 and C.albicans pattern recognition receptor Dectin1.
31982527	5	47	theme	cytokines	694:702	arg1	levels					684:689	decreased mRNA levels	669:689	decreased mRNA levels of cytokines IL23, IL22, IL17a, and IL17f, anti-candidal genes S100a8 and S100a9 and C.albicans pattern recognition receptor Dectin1	669:822	Compared with the C.albicans group, salecan reduced the migration of polymorphonuclear neutrophils (PMNs) in the vagina, decreased mRNA levels of cytokines IL23, IL22, IL17a, and IL17f, anti-candidal genes S100a8 and S100a9 and C.albicans pattern recognition receptor Dectin1.
31982527	2	48	theme	vaginal	302:308	arg1	infection					310:318	vaginal infection	302:318	vaginal infection	302:318	The purpose of this study was to evaluate the ability of a water-soluble β-glucan salecan to protect against Candida albicans (C. albicans) vaginal infection.
31982527	5	49	dep	cytokines	694:702	arg1	IL17a					716:720	IL17a	716:720	IL17a	716:720	Compared with the C.albicans group, salecan reduced the migration of polymorphonuclear neutrophils (PMNs) in the vagina, decreased mRNA levels of cytokines IL23, IL22, IL17a, and IL17f, anti-candidal genes S100a8 and S100a9 and C.albicans pattern recognition receptor Dectin1.
31982527	5	49	dep	cytokines	694:702	arg1	IL17f					727:731	IL17f	727:731	IL17f	727:731	Compared with the C.albicans group, salecan reduced the migration of polymorphonuclear neutrophils (PMNs) in the vagina, decreased mRNA levels of cytokines IL23, IL22, IL17a, and IL17f, anti-candidal genes S100a8 and S100a9 and C.albicans pattern recognition receptor Dectin1.
31982527	5	49	dep	cytokines	694:702	arg1	IL22					710:713	IL22	710:713	IL22	710:713	Compared with the C.albicans group, salecan reduced the migration of polymorphonuclear neutrophils (PMNs) in the vagina, decreased mRNA levels of cytokines IL23, IL22, IL17a, and IL17f, anti-candidal genes S100a8 and S100a9 and C.albicans pattern recognition receptor Dectin1.
31982527	5	49	dep	cytokines	694:702	arg1	IL23					704:707	IL23	704:707	IL23	704:707	Compared with the C.albicans group, salecan reduced the migration of polymorphonuclear neutrophils (PMNs) in the vagina, decreased mRNA levels of cytokines IL23, IL22, IL17a, and IL17f, anti-candidal genes S100a8 and S100a9 and C.albicans pattern recognition receptor Dectin1.
31982527	5	49	dep	cytokines	694:702	arg1	cytokines					694:702	cytokines IL23, IL22, IL17a, and IL17f	694:731	cytokines IL23, IL22, IL17a, and IL17f	694:731	Compared with the C.albicans group, salecan reduced the migration of polymorphonuclear neutrophils (PMNs) in the vagina, decreased mRNA levels of cytokines IL23, IL22, IL17a, and IL17f, anti-candidal genes S100a8 and S100a9 and C.albicans pattern recognition receptor Dectin1.
31982527	5	50	theme	neutrophils	635:645	arg1	migration					604:612	the migration	600:612	the migration of polymorphonuclear neutrophils (PMNs)	600:652	Compared with the C.albicans group, salecan reduced the migration of polymorphonuclear neutrophils (PMNs) in the vagina, decreased mRNA levels of cytokines IL23, IL22, IL17a, and IL17f, anti-candidal genes S100a8 and S100a9 and C.albicans pattern recognition receptor Dectin1.
31982527	2	51	theme	study	182:186	arg1	purpose					166:172	The purpose	162:172	The purpose of this study	162:186	The purpose of this study was to evaluate the ability of a water-soluble β-glucan salecan to protect against Candida albicans (C. albicans) vaginal infection.
31982527	7	52	contain	had	1132:1134	arg1	lavages					1093:1099	The vaginal lavages	1081:1099	The vaginal lavages from the salecan treated group	1081:1130	The vaginal lavages from the salecan treated group had more Enterococcus and its metabolite lactate.
31982527	7	52	contain	had	1132:1134	arg2	Enterococcus					1141:1152	Enterococcus	1141:1152	Enterococcus	1141:1152	The vaginal lavages from the salecan treated group had more Enterococcus and its metabolite lactate.
31982527	7	52	contain	had	1132:1134	arg2	lactate					1173:1179	its metabolite lactate	1158:1179	its metabolite lactate	1158:1179	The vaginal lavages from the salecan treated group had more Enterococcus and its metabolite lactate.
31982527	7	53	from	group	1126:1130	arg1	lavages					1093:1099	The vaginal lavages	1081:1099	The vaginal lavages from the salecan treated group	1081:1130	The vaginal lavages from the salecan treated group had more Enterococcus and its metabolite lactate.
31982527	6	54	theme	community	860:868	arg1	composition					870:880	vaginal microbial community composition	842:880	vaginal microbial community composition	842:880	The analysis for vaginal microbial community composition at different taxa levels revealed that the bacterial flora composition in the vagina of the salecan-treated mice was similar to that of the uninfected mice, and distinguished from the infected mice.
31982527	2	55	theme	water-soluble	221:233	arg1	salecan					244:250	a water-soluble β-glucan salecan	219:250	a water-soluble β-glucan salecan	219:250	The purpose of this study was to evaluate the ability of a water-soluble β-glucan salecan to protect against Candida albicans (C. albicans) vaginal infection.
31982527	8	56	theme	C.albicans	1274:1283	arg1	infection					1261:1269	vaginal infection	1253:1269	vaginal infection of C.albicans	1253:1283	Our results suggest salecan might be a potential therapeutic agent for vaginal infection of C.albicans.
31982527	8	57	theme	potential	1221:1229	arg1	agent					1243:1247	a potential therapeutic agent	1219:1247	a potential therapeutic agent for vaginal infection of C.albicans	1219:1283	Our results suggest salecan might be a potential therapeutic agent for vaginal infection of C.albicans.
31982527	5	58	theme	receptor	807:814	arg1	cytokines					694:702	cytokines IL23, IL22, IL17a, and IL17f	694:731	cytokines IL23, IL22, IL17a, and IL17f	694:731	Compared with the C.albicans group, salecan reduced the migration of polymorphonuclear neutrophils (PMNs) in the vagina, decreased mRNA levels of cytokines IL23, IL22, IL17a, and IL17f, anti-candidal genes S100a8 and S100a9 and C.albicans pattern recognition receptor Dectin1.
31982527	5	58	theme	receptor	807:814	arg1	Dectin1					816:822	C.albicans pattern recognition receptor Dectin1	776:822	C.albicans pattern recognition receptor Dectin1	776:822	Compared with the C.albicans group, salecan reduced the migration of polymorphonuclear neutrophils (PMNs) in the vagina, decreased mRNA levels of cytokines IL23, IL22, IL17a, and IL17f, anti-candidal genes S100a8 and S100a9 and C.albicans pattern recognition receptor Dectin1.
31982527	0	59	theme	albicans	66:73	arg1	infection					45:53	vaginal infection	37:53	vaginal infection of Candida albicans in mice	37:81	Soluble beta-glucan salecan improves vaginal infection of Candida albicans in mice.
31982527	3	60	from	inoculation	364:374	arg1	mice					415:418	pseudoestrus mice	402:418	pseudoestrus mice	402:418	The model was reproduced with intravaginal inoculation of yeast blastoconidia in pseudoestrus mice.
31982527	7	61	theme	metabolite	1162:1171	arg1	lactate					1173:1179	its metabolite lactate	1158:1179	its metabolite lactate	1158:1179	The vaginal lavages from the salecan treated group had more Enterococcus and its metabolite lactate.
31715589	0	0	theme	tissue-engineered	74:90	arg1	stroma					100:105	tissue-engineered corneal stroma	74:105	tissue-engineered corneal stroma	74:105	Studies on bacterial cellulose/poly(vinyl alcohol) hydrogel composites as tissue-engineered corneal stroma.
31715589	5	1	theme	stroma	659:664	arg1	material					666:673	a corneal stroma material	649:673	a corneal stroma material	649:673	It was found that the properties of BC/PVA were better suited for use as a corneal stroma material than the BC hydrogel.
31715589	7	2	dep	in	884:885	arg1	vivo					887:890	vivo	887:890	vivo	887:890	Furthermore, in the in vivo studies, the BC/PVA was transplanted intrastromally in rabbits.
31715589	0	3	from	Studies	0:6	arg1	composites					60:69	bacterial cellulose/poly(vinyl alcohol) hydrogel composites	11:69	bacterial cellulose/poly(vinyl alcohol) hydrogel composites as tissue-engineered corneal stroma	11:105	Studies on bacterial cellulose/poly(vinyl alcohol) hydrogel composites as tissue-engineered corneal stroma.
31715589	0	4	theme	corneal	92:98	arg1	stroma					100:105	tissue-engineered corneal stroma	74:105	tissue-engineered corneal stroma	74:105	Studies on bacterial cellulose/poly(vinyl alcohol) hydrogel composites as tissue-engineered corneal stroma.
31715589	9	5	theme	corneal	1260:1266	arg1	stroma					1268:1273	corneal stroma	1260:1273	corneal stroma	1260:1273	Our results demonstrate that BC/PVA was well-tolerated in the rabbit cornea, and may be a potential substitute for corneal stroma.
31715589	2	6	theme	donors	272:277	arg1	number					254:259	the limited number	242:259	the limited number of corneal donors	242:277	However, it is restricted due to the limited number of corneal donors.
31715589	4	7	theme	cellulose	484:492	arg1	composites					534:543	bacterial cellulose (BC)/poly(vinyl alcohol) (PVA) hydrogel composites	474:543	bacterial cellulose (BC)/poly(vinyl alcohol) (PVA) hydrogel composites for reconstructing the cornea	474:573	This research envisages the development of a novel tissue-engineered corneal stroma consisting of bacterial cellulose (BC)/poly(vinyl alcohol) (PVA) hydrogel composites for reconstructing the cornea.
31715589	1	8	from	solution	155:162	arg1	treatment					171:179	the treatment	167:179	the treatment of severe corneal diseases	167:206	Corneal transplantation is currently the major solution in the treatment of severe corneal diseases.
31715589	2	9	theme	corneal	264:270	arg1	donors					272:277	corneal donors	264:277	corneal donors	264:277	However, it is restricted due to the limited number of corneal donors.
31715589	8	10	theme	histological	1118:1129	arg1	examinations					1131:1142	the clinical and histological examinations	1101:1142	the clinical and histological examinations	1101:1142	After four weeks, the cornea remained almost transparent, and without obvious inflammation, sensitization or neovascularization, as confirmed by the clinical and histological examinations.
31715589	9	11	dep	potential	1235:1243	arg1	substitute					1245:1254	substitute	1245:1254	substitute for corneal stroma	1245:1273	Our results demonstrate that BC/PVA was well-tolerated in the rabbit cornea, and may be a potential substitute for corneal stroma.
31715589	5	12	theme	BC/PVA	612:617	arg1	properties					598:607	the properties	594:607	the properties of BC/PVA	594:617	It was found that the properties of BC/PVA were better suited for use as a corneal stroma material than the BC hydrogel.
31715589	5	12	theme	BC/PVA	612:617	arg1	better					624:629	better	624:629	better	624:629	It was found that the properties of BC/PVA were better suited for use as a corneal stroma material than the BC hydrogel.
31715589	1	13	theme	severe	184:189	arg1	diseases					199:206	severe corneal diseases	184:206	severe corneal diseases	184:206	Corneal transplantation is currently the major solution in the treatment of severe corneal diseases.
31715589	4	14	theme	vinyl	504:508	arg1	cellulose					484:492	bacterial cellulose	474:492	bacterial cellulose (BC)/poly(vinyl alcohol) (PVA) hydrogel composites for reconstructing the cornea	474:573	This research envisages the development of a novel tissue-engineered corneal stroma consisting of bacterial cellulose (BC)/poly(vinyl alcohol) (PVA) hydrogel composites for reconstructing the cornea.
31715589	4	14	theme	vinyl	504:508	arg1	alcohol					510:516	vinyl alcohol	504:516	vinyl alcohol	504:516	This research envisages the development of a novel tissue-engineered corneal stroma consisting of bacterial cellulose (BC)/poly(vinyl alcohol) (PVA) hydrogel composites for reconstructing the cornea.
31715589	1	15	theme	corneal	191:197	arg1	diseases					199:206	severe corneal diseases	184:206	severe corneal diseases	184:206	Corneal transplantation is currently the major solution in the treatment of severe corneal diseases.
31715589	0	16	theme	cellulose/poly	21:34	arg1	composites					60:69	bacterial cellulose/poly(vinyl alcohol) hydrogel composites	11:69	bacterial cellulose/poly(vinyl alcohol) hydrogel composites as tissue-engineered corneal stroma	11:105	Studies on bacterial cellulose/poly(vinyl alcohol) hydrogel composites as tissue-engineered corneal stroma.
31715589	4	17	theme	hydrogel	525:532	arg1	composites					534:543	bacterial cellulose (BC)/poly(vinyl alcohol) (PVA) hydrogel composites	474:543	bacterial cellulose (BC)/poly(vinyl alcohol) (PVA) hydrogel composites for reconstructing the cornea	474:573	This research envisages the development of a novel tissue-engineered corneal stroma consisting of bacterial cellulose (BC)/poly(vinyl alcohol) (PVA) hydrogel composites for reconstructing the cornea.
31715589	8	18	theme	obvious	1026:1032	arg1	inflammation					1034:1045	obvious inflammation	1026:1045	obvious inflammation	1026:1045	After four weeks, the cornea remained almost transparent, and without obvious inflammation, sensitization or neovascularization, as confirmed by the clinical and histological examinations.
31715589	7	19	theme	in	884:885	arg1	studies					892:898	the in vivo studies	880:898	the in vivo studies	880:898	Furthermore, in the in vivo studies, the BC/PVA was transplanted intrastromally in rabbits.
31715589	1	20	theme	diseases	199:206	arg1	treatment					171:179	the treatment	167:179	the treatment of severe corneal diseases	167:206	Corneal transplantation is currently the major solution in the treatment of severe corneal diseases.
31715589	0	21	theme	bacterial	11:19	arg1	composites					60:69	bacterial cellulose/poly(vinyl alcohol) hydrogel composites	11:69	bacterial cellulose/poly(vinyl alcohol) hydrogel composites as tissue-engineered corneal stroma	11:105	Studies on bacterial cellulose/poly(vinyl alcohol) hydrogel composites as tissue-engineered corneal stroma.
31715589	3	22	dep	potential	312:320	arg1	substitute					322:331	substitute	322:331	substitute	322:331	A tissue-engineered cornea is a potential substitute which could help overcome this limitation.
31715589	6	23	theme	excellent	819:827	arg1	biocompatibility					829:844	excellent biocompatibility	819:844	excellent biocompatibility	819:844	The human corneal stromal cells (hCSCs) were used to evaluate the cytotoxicity of the materials, wherein BC/PVA displayed excellent biocompatibility with these cells.
31715589	1	24	theme	Corneal	108:114	arg1	solution					155:162	the major solution	145:162	the major solution in the treatment of severe corneal diseases	145:206	Corneal transplantation is currently the major solution in the treatment of severe corneal diseases.
31715589	1	24	theme	Corneal	108:114	arg1	transplantation					116:130	Corneal transplantation	108:130	Corneal transplantation	108:130	Corneal transplantation is currently the major solution in the treatment of severe corneal diseases.
31715589	0	25	theme	vinyl	36:40	arg1	composites					60:69	bacterial cellulose/poly(vinyl alcohol) hydrogel composites	11:69	bacterial cellulose/poly(vinyl alcohol) hydrogel composites as tissue-engineered corneal stroma	11:105	Studies on bacterial cellulose/poly(vinyl alcohol) hydrogel composites as tissue-engineered corneal stroma.
31715589	9	26	from	well-tolerated	1185:1198	arg1	cornea					1214:1219	the rabbit cornea	1203:1219	the rabbit cornea	1203:1219	Our results demonstrate that BC/PVA was well-tolerated in the rabbit cornea, and may be a potential substitute for corneal stroma.
31715589	9	27	from	cornea	1214:1219	arg1	well-tolerated					1185:1198	well-tolerated	1185:1198	well-tolerated	1185:1198	Our results demonstrate that BC/PVA was well-tolerated in the rabbit cornea, and may be a potential substitute for corneal stroma.
31715589	4	28	theme	stroma	453:458	arg1	development					404:414	the development	400:414	the development of a novel tissue-engineered corneal stroma consisting of bacterial cellulose (BC)/poly(vinyl alcohol) (PVA) hydrogel composites for reconstructing the cornea	400:573	This research envisages the development of a novel tissue-engineered corneal stroma consisting of bacterial cellulose (BC)/poly(vinyl alcohol) (PVA) hydrogel composites for reconstructing the cornea.
31715589	6	29	used	used	742:745	arg2	hCSCs					730:734	hCSCs	730:734	hCSCs	730:734	The human corneal stromal cells (hCSCs) were used to evaluate the cytotoxicity of the materials, wherein BC/PVA displayed excellent biocompatibility with these cells.
31715589	6	29	used	used	742:745	arg2	cells					723:727	The human corneal stromal cells	697:727	The human corneal stromal cells (hCSCs)	697:735	The human corneal stromal cells (hCSCs) were used to evaluate the cytotoxicity of the materials, wherein BC/PVA displayed excellent biocompatibility with these cells.
31715589	5	30	theme	BC	684:685	arg1	hydrogel					687:694	the BC hydrogel	680:694	the BC hydrogel	680:694	It was found that the properties of BC/PVA were better suited for use as a corneal stroma material than the BC hydrogel.
31715589	4	31	theme	bacterial	474:482	arg1	BC					495:496	BC	495:496	BC	495:496	This research envisages the development of a novel tissue-engineered corneal stroma consisting of bacterial cellulose (BC)/poly(vinyl alcohol) (PVA) hydrogel composites for reconstructing the cornea.
31715589	4	31	theme	bacterial	474:482	arg1	PVA					520:522	PVA	520:522	PVA	520:522	This research envisages the development of a novel tissue-engineered corneal stroma consisting of bacterial cellulose (BC)/poly(vinyl alcohol) (PVA) hydrogel composites for reconstructing the cornea.
31715589	4	31	theme	bacterial	474:482	arg1	cellulose					484:492	bacterial cellulose	474:492	bacterial cellulose (BC)/poly(vinyl alcohol) (PVA) hydrogel composites for reconstructing the cornea	474:573	This research envisages the development of a novel tissue-engineered corneal stroma consisting of bacterial cellulose (BC)/poly(vinyl alcohol) (PVA) hydrogel composites for reconstructing the cornea.
31715589	4	31	theme	bacterial	474:482	arg1	alcohol					510:516	vinyl alcohol	504:516	vinyl alcohol	504:516	This research envisages the development of a novel tissue-engineered corneal stroma consisting of bacterial cellulose (BC)/poly(vinyl alcohol) (PVA) hydrogel composites for reconstructing the cornea.
31715589	6	32	theme	wherein	794:800	arg1	BC/PVA					802:807	wherein BC/PVA	794:807	wherein BC/PVA	794:807	The human corneal stromal cells (hCSCs) were used to evaluate the cytotoxicity of the materials, wherein BC/PVA displayed excellent biocompatibility with these cells.
31715589	0	33	theme	alcohol	42:48	arg1	composites					60:69	bacterial cellulose/poly(vinyl alcohol) hydrogel composites	11:69	bacterial cellulose/poly(vinyl alcohol) hydrogel composites as tissue-engineered corneal stroma	11:105	Studies on bacterial cellulose/poly(vinyl alcohol) hydrogel composites as tissue-engineered corneal stroma.
31715589	4	34	theme	novel	421:425	arg1	stroma					453:458	a novel tissue-engineered corneal stroma	419:458	a novel tissue-engineered corneal stroma consisting of bacterial cellulose (BC)/poly(vinyl alcohol) (PVA) hydrogel composites for reconstructing the cornea	419:573	This research envisages the development of a novel tissue-engineered corneal stroma consisting of bacterial cellulose (BC)/poly(vinyl alcohol) (PVA) hydrogel composites for reconstructing the cornea.
31715589	6	35	theme	stromal	715:721	arg1	hCSCs					730:734	hCSCs	730:734	hCSCs	730:734	The human corneal stromal cells (hCSCs) were used to evaluate the cytotoxicity of the materials, wherein BC/PVA displayed excellent biocompatibility with these cells.
31715589	6	35	theme	stromal	715:721	arg1	cells					723:727	The human corneal stromal cells	697:727	The human corneal stromal cells (hCSCs)	697:735	The human corneal stromal cells (hCSCs) were used to evaluate the cytotoxicity of the materials, wherein BC/PVA displayed excellent biocompatibility with these cells.
31715589	9	36	theme	rabbit	1207:1212	arg1	cornea					1214:1219	the rabbit cornea	1203:1219	the rabbit cornea	1203:1219	Our results demonstrate that BC/PVA was well-tolerated in the rabbit cornea, and may be a potential substitute for corneal stroma.
31715589	5	37	theme	corneal	651:657	arg1	material					666:673	a corneal stroma material	649:673	a corneal stroma material	649:673	It was found that the properties of BC/PVA were better suited for use as a corneal stroma material than the BC hydrogel.
31715589	6	38	theme	materials	783:791	arg1	cytotoxicity					763:774	the cytotoxicity	759:774	the cytotoxicity of the materials	759:791	The human corneal stromal cells (hCSCs) were used to evaluate the cytotoxicity of the materials, wherein BC/PVA displayed excellent biocompatibility with these cells.
31715589	6	39	theme	corneal	707:713	arg1	hCSCs					730:734	hCSCs	730:734	hCSCs	730:734	The human corneal stromal cells (hCSCs) were used to evaluate the cytotoxicity of the materials, wherein BC/PVA displayed excellent biocompatibility with these cells.
31715589	6	39	theme	corneal	707:713	arg1	cells					723:727	The human corneal stromal cells	697:727	The human corneal stromal cells (hCSCs)	697:735	The human corneal stromal cells (hCSCs) were used to evaluate the cytotoxicity of the materials, wherein BC/PVA displayed excellent biocompatibility with these cells.
31715589	3	40	theme	tissue-engineered	282:298	arg1	cornea					300:305	A tissue-engineered cornea	280:305	A tissue-engineered cornea	280:305	A tissue-engineered cornea is a potential substitute which could help overcome this limitation.
31715589	3	40	theme	tissue-engineered	282:298	arg1	potential					312:320	a potential substitute	310:331	a potential substitute which could help overcome this limitation	310:373	A tissue-engineered cornea is a potential substitute which could help overcome this limitation.
31715589	4	41	theme	corneal	445:451	arg1	stroma					453:458	a novel tissue-engineered corneal stroma	419:458	a novel tissue-engineered corneal stroma consisting of bacterial cellulose (BC)/poly(vinyl alcohol) (PVA) hydrogel composites for reconstructing the cornea	419:573	This research envisages the development of a novel tissue-engineered corneal stroma consisting of bacterial cellulose (BC)/poly(vinyl alcohol) (PVA) hydrogel composites for reconstructing the cornea.
31715589	2	42	theme	limited	246:252	arg1	number					254:259	the limited number	242:259	the limited number of corneal donors	242:277	However, it is restricted due to the limited number of corneal donors.
31715589	6	43	theme	human	701:705	arg1	hCSCs					730:734	hCSCs	730:734	hCSCs	730:734	The human corneal stromal cells (hCSCs) were used to evaluate the cytotoxicity of the materials, wherein BC/PVA displayed excellent biocompatibility with these cells.
31715589	6	43	theme	human	701:705	arg1	cells					723:727	The human corneal stromal cells	697:727	The human corneal stromal cells (hCSCs)	697:735	The human corneal stromal cells (hCSCs) were used to evaluate the cytotoxicity of the materials, wherein BC/PVA displayed excellent biocompatibility with these cells.
31715589	4	44	theme	tissue-engineered	427:443	arg1	stroma					453:458	a novel tissue-engineered corneal stroma	419:458	a novel tissue-engineered corneal stroma consisting of bacterial cellulose (BC)/poly(vinyl alcohol) (PVA) hydrogel composites for reconstructing the cornea	419:573	This research envisages the development of a novel tissue-engineered corneal stroma consisting of bacterial cellulose (BC)/poly(vinyl alcohol) (PVA) hydrogel composites for reconstructing the cornea.
31715589	1	45	theme	major	149:153	arg1	solution					155:162	the major solution	145:162	the major solution in the treatment of severe corneal diseases	145:206	Corneal transplantation is currently the major solution in the treatment of severe corneal diseases.
31715589	1	45	theme	major	149:153	arg1	transplantation					116:130	Corneal transplantation	108:130	Corneal transplantation	108:130	Corneal transplantation is currently the major solution in the treatment of severe corneal diseases.
31715589	0	46	theme	hydrogel	51:58	arg1	composites					60:69	bacterial cellulose/poly(vinyl alcohol) hydrogel composites	11:69	bacterial cellulose/poly(vinyl alcohol) hydrogel composites as tissue-engineered corneal stroma	11:105	Studies on bacterial cellulose/poly(vinyl alcohol) hydrogel composites as tissue-engineered corneal stroma.
31715589	8	47	theme	clinical	1105:1112	arg1	examinations					1131:1142	the clinical and histological examinations	1101:1142	the clinical and histological examinations	1101:1142	After four weeks, the cornea remained almost transparent, and without obvious inflammation, sensitization or neovascularization, as confirmed by the clinical and histological examinations.
34602414	2	0	theme	release	352:358	arg1	pattern					360:366	release pattern	352:366	release pattern	352:366	Compressibility behavior of different powders and mixtures of formulations and release pattern of any tablets are characteristic measures to define prerequisite quality attributes of any compressed formulations.
34602414	6	1	dep	%	1248:1248	arg1	to					1234:1235	to	1234:1235	to	1234:1235	Nine formulations were developed from F1 to F9 with Crospovidone (superdisintegrant) as (X1) (-α=1.17% to ± α=6.83%) and microcrystalline cellulose (Avicel 102, Filler/binder) as X2 (-α = 29.82% to ± α = 65.18%).
34602414	4	2	theme	Later	620:624	arg1	process					626:632	Later process	620:632	Later process	620:632	Later process offer fewer processing steps and agreeable release profile with acceptable quality parameters and hence preferred over granulation method.
34602414	2	3	theme	Compressibility	273:287	arg1	behavior					289:296	Compressibility behavior	273:296	Compressibility behavior of different powders	273:317	Compressibility behavior of different powders and mixtures of formulations and release pattern of any tablets are characteristic measures to define prerequisite quality attributes of any compressed formulations.
34602414	1	4	theme	significant	252:262	arg1	concern					264:270	significant concern	252:270	significant concern	252:270	In order for preparing a solid oral dosage form, tablet quality is of significant concern.
34602414	6	5	theme	α	1239:1239	arg1	%					1248:1248	-α = 29.82% to ± α = 65.18%	1222:1248	-α = 29.82% to ± α = 65.18%	1222:1248	Nine formulations were developed from F1 to F9 with Crospovidone (superdisintegrant) as (X1) (-α=1.17% to ± α=6.83%) and microcrystalline cellulose (Avicel 102, Filler/binder) as X2 (-α = 29.82% to ± α = 65.18%).
34602414	0	6	theme	Formulation	0:10	arg1	development					12:22	Formulation development	0:22	Formulation development of directly compressible mebevarine tablets using superdisintegrant: A way to investigate quality atributes, in vitro release kinetics and stability profile.	0:180	Formulation development of directly compressible mebevarine tablets using superdisintegrant: A way to investigate quality atributes, in vitro release kinetics and stability profile.
34602414	11	7	from	validity	1827:1834	arg1	months					1859:1864	months	1859:1864	months	1859:1864	The Trial formulations F2, F4, F6 and F8 were also studied on accelerated conditions (40±5ºC 75%±5% RH) for stability tests and validity of the formulations in months were also determined between 35-39 months.
34602414	4	8	theme	quality	709:715	arg1	parameters					717:726	acceptable quality parameters	698:726	acceptable quality parameters and hence preferred over granulation method	698:770	Later process offer fewer processing steps and agreeable release profile with acceptable quality parameters and hence preferred over granulation method.
34602414	2	9	theme	powders	311:317	arg1	mixtures					323:330	mixtures	323:330	mixtures of formulations and release pattern of any tablets	323:381	Compressibility behavior of different powders and mixtures of formulations and release pattern of any tablets are characteristic measures to define prerequisite quality attributes of any compressed formulations.
34602414	2	9	theme	powders	311:317	arg1	behavior					289:296	Compressibility behavior	273:296	Compressibility behavior of different powders	273:317	Compressibility behavior of different powders and mixtures of formulations and release pattern of any tablets are characteristic measures to define prerequisite quality attributes of any compressed formulations.
34602414	6	10	with	F9	1083:1084	arg1	X1					1128:1129	X1	1128:1129	X1	1128:1129	Nine formulations were developed from F1 to F9 with Crospovidone (superdisintegrant) as (X1) (-α=1.17% to ± α=6.83%) and microcrystalline cellulose (Avicel 102, Filler/binder) as X2 (-α = 29.82% to ± α = 65.18%).
34602414	6	10	with	F9	1083:1084	arg1	as					1124:1125	as	1124:1125	as	1124:1125	Nine formulations were developed from F1 to F9 with Crospovidone (superdisintegrant) as (X1) (-α=1.17% to ± α=6.83%) and microcrystalline cellulose (Avicel 102, Filler/binder) as X2 (-α = 29.82% to ± α = 65.18%).
34602414	6	11	theme	microcrystalline	1160:1175	arg1	cellulose					1177:1185	microcrystalline cellulose	1160:1185	microcrystalline cellulose	1160:1185	Nine formulations were developed from F1 to F9 with Crospovidone (superdisintegrant) as (X1) (-α=1.17% to ± α=6.83%) and microcrystalline cellulose (Avicel 102, Filler/binder) as X2 (-α = 29.82% to ± α = 65.18%).
34602414	10	12	theme	r2	1645:1646	arg1	model					1638:1642	Hixon-crowell's model	1622:1642	Hixon-crowell's model (r2 = 0.995) followed by Weibull's model (r2 = 0.985)	1622:1696	Best fit model was found to be Hixon-crowell's model (r2 = 0.995) followed by Weibull's model (r2 = 0.985).
34602414	10	12	theme	r2	1645:1646	arg1	0.995					1650:1654	r2 = 0.995	1645:1654	r2 = 0.995	1645:1654	Best fit model was found to be Hixon-crowell's model (r2 = 0.995) followed by Weibull's model (r2 = 0.985).
34602414	6	13	theme	%	1232:1232	arg1	%					1248:1248	-α = 29.82% to ± α = 65.18%	1222:1248	-α = 29.82% to ± α = 65.18%	1222:1248	Nine formulations were developed from F1 to F9 with Crospovidone (superdisintegrant) as (X1) (-α=1.17% to ± α=6.83%) and microcrystalline cellulose (Avicel 102, Filler/binder) as X2 (-α = 29.82% to ± α = 65.18%).
34602414	11	14	theme	accelerated	1761:1771	arg1	conditions					1773:1782	accelerated conditions	1761:1782	accelerated conditions (40±5ºC 75%±5% RH)	1761:1801	The Trial formulations F2, F4, F6 and F8 were also studied on accelerated conditions (40±5ºC 75%±5% RH) for stability tests and validity of the formulations in months were also determined between 35-39 months.
34602414	11	14	theme	accelerated	1761:1771	arg1	%					1797:1797	40±5ºC 75%±5% RH	1785:1800	40±5ºC 75%±5% RH	1785:1800	The Trial formulations F2, F4, F6 and F8 were also studied on accelerated conditions (40±5ºC 75%±5% RH) for stability tests and validity of the formulations in months were also determined between 35-39 months.
34602414	4	15	theme	release	677:683	arg1	profile					685:691	agreeable release profile	667:691	agreeable release profile	667:691	Later process offer fewer processing steps and agreeable release profile with acceptable quality parameters and hence preferred over granulation method.
34602414	5	16	theme	filler	924:929	arg1	binders					931:937	filler binders	924:937	filler binders	924:937	In this investigation Mebeverine hydrochloride an anti-muscarinic drug is studied for compression and release behavior using various concentrations of filler binders and disintegrants via rotatable central composite design (CCRD) option of design expert (software).
34602414	6	17	theme	=	1225:1225	arg1	%					1248:1248	-α = 29.82% to ± α = 65.18%	1222:1248	-α = 29.82% to ± α = 65.18%	1222:1248	Nine formulations were developed from F1 to F9 with Crospovidone (superdisintegrant) as (X1) (-α=1.17% to ± α=6.83%) and microcrystalline cellulose (Avicel 102, Filler/binder) as X2 (-α = 29.82% to ± α = 65.18%).
34602414	4	18	theme	granulation	753:763	arg1	method					765:770	granulation method	753:770	granulation method	753:770	Later process offer fewer processing steps and agreeable release profile with acceptable quality parameters and hence preferred over granulation method.
34602414	11	19	theme	Trial	1703:1707	arg1	F6					1730:1731	F6	1730:1731	F6	1730:1731	The Trial formulations F2, F4, F6 and F8 were also studied on accelerated conditions (40±5ºC 75%±5% RH) for stability tests and validity of the formulations in months were also determined between 35-39 months.
34602414	11	19	theme	Trial	1703:1707	arg1	formulations					1709:1720	The Trial formulations	1699:1720	The Trial formulations F2, F4, F6 and F8	1699:1738	The Trial formulations F2, F4, F6 and F8 were also studied on accelerated conditions (40±5ºC 75%±5% RH) for stability tests and validity of the formulations in months were also determined between 35-39 months.
34602414	11	19	theme	Trial	1703:1707	arg1	F2					1722:1723	F2	1722:1723	F2	1722:1723	The Trial formulations F2, F4, F6 and F8 were also studied on accelerated conditions (40±5ºC 75%±5% RH) for stability tests and validity of the formulations in months were also determined between 35-39 months.
34602414	11	19	theme	Trial	1703:1707	arg1	F8					1737:1738	F8	1737:1738	F8	1737:1738	The Trial formulations F2, F4, F6 and F8 were also studied on accelerated conditions (40±5ºC 75%±5% RH) for stability tests and validity of the formulations in months were also determined between 35-39 months.
34602414	5	20	theme	CCRD	997:1000	arg1	option					1003:1008	rotatable central composite design (CCRD) option	961:1008	rotatable central composite design (CCRD) option of design expert (software)	961:1036	In this investigation Mebeverine hydrochloride an anti-muscarinic drug is studied for compression and release behavior using various concentrations of filler binders and disintegrants via rotatable central composite design (CCRD) option of design expert (software).
34602414	10	21	dep	=	1689:1689	arg1	0.985					1691:1695	0.985	1691:1695	0.985	1691:1695	Best fit model was found to be Hixon-crowell's model (r2 = 0.995) followed by Weibull's model (r2 = 0.985).
34602414	0	22	theme	stability	163:171	arg1	profile					173:179	stability profile	163:179	stability profile	163:179	Formulation development of directly compressible mebevarine tablets using superdisintegrant: A way to investigate quality atributes, in vitro release kinetics and stability profile.
34602414	8	23	theme	powder	1386:1391	arg1	blends					1393:1398	powder blends	1386:1398	powder blends	1386:1398	The performance of powder blends and formulations was analyzed by micromeritic and physico-chemical and assessments.
34602414	11	24	theme	%	1794:1794	arg1	conditions					1773:1782	accelerated conditions	1761:1782	accelerated conditions (40±5ºC 75%±5% RH)	1761:1801	The Trial formulations F2, F4, F6 and F8 were also studied on accelerated conditions (40±5ºC 75%±5% RH) for stability tests and validity of the formulations in months were also determined between 35-39 months.
34602414	11	24	theme	%	1794:1794	arg1	%					1797:1797	40±5ºC 75%±5% RH	1785:1800	40±5ºC 75%±5% RH	1785:1800	The Trial formulations F2, F4, F6 and F8 were also studied on accelerated conditions (40±5ºC 75%±5% RH) for stability tests and validity of the formulations in months were also determined between 35-39 months.
34602414	5	25	theme	central	971:977	arg1	option					1003:1008	rotatable central composite design (CCRD) option	961:1008	rotatable central composite design (CCRD) option of design expert (software)	961:1036	In this investigation Mebeverine hydrochloride an anti-muscarinic drug is studied for compression and release behavior using various concentrations of filler binders and disintegrants via rotatable central composite design (CCRD) option of design expert (software).
34602414	5	26	theme	design	989:994	arg1	option					1003:1008	rotatable central composite design (CCRD) option	961:1008	rotatable central composite design (CCRD) option of design expert (software)	961:1036	In this investigation Mebeverine hydrochloride an anti-muscarinic drug is studied for compression and release behavior using various concentrations of filler binders and disintegrants via rotatable central composite design (CCRD) option of design expert (software).
34602414	6	27	dep	developed	1062:1070	arg1	%					1248:1248	-α = 29.82% to ± α = 65.18%	1222:1248	-α = 29.82% to ± α = 65.18%	1222:1248	Nine formulations were developed from F1 to F9 with Crospovidone (superdisintegrant) as (X1) (-α=1.17% to ± α=6.83%) and microcrystalline cellulose (Avicel 102, Filler/binder) as X2 (-α = 29.82% to ± α = 65.18%).
34602414	6	27	dep	developed	1062:1070	arg1	Avicel					1188:1193	Avicel 102	1188:1197	Avicel 102	1188:1197	Nine formulations were developed from F1 to F9 with Crospovidone (superdisintegrant) as (X1) (-α=1.17% to ± α=6.83%) and microcrystalline cellulose (Avicel 102, Filler/binder) as X2 (-α = 29.82% to ± α = 65.18%).
34602414	7	28	theme	dependent	1337:1345	arg1	Hardness					1289:1296	Hardness	1289:1296	Hardness in (kg) as (R2)	1289:1312	Disintegration Time (DT) as (R1) and Hardness in (kg) as (R2) were determined as two dependent response variables.
34602414	7	28	theme	dependent	1337:1345	arg1	variables					1356:1364	two dependent response variables	1333:1364	two dependent response variables	1333:1364	Disintegration Time (DT) as (R1) and Hardness in (kg) as (R2) were determined as two dependent response variables.
34602414	7	28	theme	dependent	1337:1345	arg1	Time					1267:1270	Disintegration Time	1252:1270	Disintegration Time (DT) as (R1)	1252:1283	Disintegration Time (DT) as (R1) and Hardness in (kg) as (R2) were determined as two dependent response variables.
34602414	11	29	theme	±5	1795:1796	arg1	conditions					1773:1782	accelerated conditions	1761:1782	accelerated conditions (40±5ºC 75%±5% RH)	1761:1801	The Trial formulations F2, F4, F6 and F8 were also studied on accelerated conditions (40±5ºC 75%±5% RH) for stability tests and validity of the formulations in months were also determined between 35-39 months.
34602414	11	29	theme	±5	1795:1796	arg1	%					1797:1797	40±5ºC 75%±5% RH	1785:1800	40±5ºC 75%±5% RH	1785:1800	The Trial formulations F2, F4, F6 and F8 were also studied on accelerated conditions (40±5ºC 75%±5% RH) for stability tests and validity of the formulations in months were also determined between 35-39 months.
34602414	5	30	theme	Mebeverine	795:804	arg1	hydrochloride					806:818	this investigation Mebeverine hydrochloride	776:818	this investigation Mebeverine hydrochloride	776:818	In this investigation Mebeverine hydrochloride an anti-muscarinic drug is studied for compression and release behavior using various concentrations of filler binders and disintegrants via rotatable central composite design (CCRD) option of design expert (software).
34602414	9	31	theme	Dissolution	1484:1494	arg1	comparisons					1496:1506	Dissolution comparisons	1484:1506	Dissolution comparisons	1484:1506	Dissolution comparisons were statistically analyzed by ANOVA and model dependent and in-dependent methods.
34602414	7	32	from	Hardness	1289:1296	arg1	R2					1310:1311	R2	1310:1311	R2	1310:1311	Disintegration Time (DT) as (R1) and Hardness in (kg) as (R2) were determined as two dependent response variables.
34602414	7	32	from	Hardness	1289:1296	arg1	as					1306:1307	as	1306:1307	as	1306:1307	Disintegration Time (DT) as (R1) and Hardness in (kg) as (R2) were determined as two dependent response variables.
34602414	2	33	theme	compressed	460:469	arg1	formulations					471:482	any compressed formulations	456:482	any compressed formulations	456:482	Compressibility behavior of different powders and mixtures of formulations and release pattern of any tablets are characteristic measures to define prerequisite quality attributes of any compressed formulations.
34602414	10	34	theme	Best	1591:1594	arg1	model					1600:1604	Best fit model	1591:1604	Best fit model	1591:1604	Best fit model was found to be Hixon-crowell's model (r2 = 0.995) followed by Weibull's model (r2 = 0.985).
34602414	5	35	theme	release	875:881	arg1	behavior					883:890	release behavior	875:890	release behavior	875:890	In this investigation Mebeverine hydrochloride an anti-muscarinic drug is studied for compression and release behavior using various concentrations of filler binders and disintegrants via rotatable central composite design (CCRD) option of design expert (software).
34602414	5	36	theme	design	1013:1018	arg1	software					1028:1035	design expert (software)	1013:1036	design expert (software)	1013:1036	In this investigation Mebeverine hydrochloride an anti-muscarinic drug is studied for compression and release behavior using various concentrations of filler binders and disintegrants via rotatable central composite design (CCRD) option of design expert (software).
34602414	2	37	theme	quality	434:440	arg1	attributes					442:451	prerequisite quality attributes	421:451	prerequisite quality attributes of any compressed formulations	421:482	Compressibility behavior of different powders and mixtures of formulations and release pattern of any tablets are characteristic measures to define prerequisite quality attributes of any compressed formulations.
34602414	11	38	theme	40±5ºC	1785:1790	arg1	conditions					1773:1782	accelerated conditions	1761:1782	accelerated conditions (40±5ºC 75%±5% RH)	1761:1801	The Trial formulations F2, F4, F6 and F8 were also studied on accelerated conditions (40±5ºC 75%±5% RH) for stability tests and validity of the formulations in months were also determined between 35-39 months.
34602414	11	38	theme	40±5ºC	1785:1790	arg1	%					1797:1797	40±5ºC 75%±5% RH	1785:1800	40±5ºC 75%±5% RH	1785:1800	The Trial formulations F2, F4, F6 and F8 were also studied on accelerated conditions (40±5ºC 75%±5% RH) for stability tests and validity of the formulations in months were also determined between 35-39 months.
34602414	0	39	theme	compressible	36:47	arg1	tablets					60:66	directly compressible mebevarine tablets	27:66	directly compressible mebevarine tablets using superdisintegrant	27:90	Formulation development of directly compressible mebevarine tablets using superdisintegrant: A way to investigate quality atributes, in vitro release kinetics and stability profile.
34602414	3	40	theme	major	509:513	arg1	methods					515:521	two major methods	505:521	two major methods that can be adopted for the preparation of tablets including granulation and direct compression	505:617	There are basically two major methods that can be adopted for the preparation of tablets including granulation and direct compression.
34602414	0	41	theme	tablets	60:66	arg1	development					12:22	Formulation development	0:22	Formulation development of directly compressible mebevarine tablets using superdisintegrant: A way to investigate quality atributes, in vitro release kinetics and stability profile.	0:180	Formulation development of directly compressible mebevarine tablets using superdisintegrant: A way to investigate quality atributes, in vitro release kinetics and stability profile.
34602414	7	42	dep	R1	1281:1282	arg1	as					1277:1278	as	1277:1278	as	1277:1278	Disintegration Time (DT) as (R1) and Hardness in (kg) as (R2) were determined as two dependent response variables.
34602414	11	43	from	months	1859:1864	arg1	validity					1827:1834	validity	1827:1834	validity of the formulations in months	1827:1864	The Trial formulations F2, F4, F6 and F8 were also studied on accelerated conditions (40±5ºC 75%±5% RH) for stability tests and validity of the formulations in months were also determined between 35-39 months.
34602414	1	44	theme	dosage	218:223	arg1	form					225:228	a solid oral dosage form	205:228	a solid oral dosage form	205:228	In order for preparing a solid oral dosage form, tablet quality is of significant concern.
34602414	6	45	theme	±	1145:1145	arg1	%					1153:1153	± α=6.83%	1145:1153	-α=1.17% to ± α=6.83%	1133:1153	Nine formulations were developed from F1 to F9 with Crospovidone (superdisintegrant) as (X1) (-α=1.17% to ± α=6.83%) and microcrystalline cellulose (Avicel 102, Filler/binder) as X2 (-α = 29.82% to ± α = 65.18%).
34602414	4	46	theme	fewer	640:644	arg1	steps					657:661	fewer processing steps	640:661	fewer processing steps	640:661	Later process offer fewer processing steps and agreeable release profile with acceptable quality parameters and hence preferred over granulation method.
34602414	2	47	theme	pattern	360:366	arg1	mixtures					323:330	mixtures	323:330	mixtures of formulations and release pattern of any tablets	323:381	Compressibility behavior of different powders and mixtures of formulations and release pattern of any tablets are characteristic measures to define prerequisite quality attributes of any compressed formulations.
34602414	2	47	theme	pattern	360:366	arg1	behavior					289:296	Compressibility behavior	273:296	Compressibility behavior of different powders	273:317	Compressibility behavior of different powders and mixtures of formulations and release pattern of any tablets are characteristic measures to define prerequisite quality attributes of any compressed formulations.
34602414	11	48	from	formulations	1843:1854	arg1	months					1859:1864	months	1859:1864	months	1859:1864	The Trial formulations F2, F4, F6 and F8 were also studied on accelerated conditions (40±5ºC 75%±5% RH) for stability tests and validity of the formulations in months were also determined between 35-39 months.
34602414	11	49	theme	formulations	1843:1854	arg1	validity					1827:1834	validity	1827:1834	validity of the formulations in months	1827:1864	The Trial formulations F2, F4, F6 and F8 were also studied on accelerated conditions (40±5ºC 75%±5% RH) for stability tests and validity of the formulations in months were also determined between 35-39 months.
34602414	5	50	theme	composite	979:987	arg1	option					1003:1008	rotatable central composite design (CCRD) option	961:1008	rotatable central composite design (CCRD) option of design expert (software)	961:1036	In this investigation Mebeverine hydrochloride an anti-muscarinic drug is studied for compression and release behavior using various concentrations of filler binders and disintegrants via rotatable central composite design (CCRD) option of design expert (software).
34602414	6	51	theme	=	1241:1241	arg1	%					1248:1248	-α = 29.82% to ± α = 65.18%	1222:1248	-α = 29.82% to ± α = 65.18%	1222:1248	Nine formulations were developed from F1 to F9 with Crospovidone (superdisintegrant) as (X1) (-α=1.17% to ± α=6.83%) and microcrystalline cellulose (Avicel 102, Filler/binder) as X2 (-α = 29.82% to ± α = 65.18%).
34602414	5	52	theme	binders	931:937	arg1	concentrations					906:919	various concentrations	898:919	various concentrations of filler binders and disintegrants	898:955	In this investigation Mebeverine hydrochloride an anti-muscarinic drug is studied for compression and release behavior using various concentrations of filler binders and disintegrants via rotatable central composite design (CCRD) option of design expert (software).
34602414	7	53	theme	response	1347:1354	arg1	Hardness					1289:1296	Hardness	1289:1296	Hardness in (kg) as (R2)	1289:1312	Disintegration Time (DT) as (R1) and Hardness in (kg) as (R2) were determined as two dependent response variables.
34602414	7	53	theme	response	1347:1354	arg1	variables					1356:1364	two dependent response variables	1333:1364	two dependent response variables	1333:1364	Disintegration Time (DT) as (R1) and Hardness in (kg) as (R2) were determined as two dependent response variables.
34602414	7	53	theme	response	1347:1354	arg1	Time					1267:1270	Disintegration Time	1252:1270	Disintegration Time (DT) as (R1)	1252:1283	Disintegration Time (DT) as (R1) and Hardness in (kg) as (R2) were determined as two dependent response variables.
34602414	1	54	from	concern	264:270	arg1	order					185:189	order	185:189	order for preparing a solid oral dosage form	185:228	In order for preparing a solid oral dosage form, tablet quality is of significant concern.
34602414	10	55	theme	=	1689:1689	arg1	r2					1686:1687	r2	1686:1687	r2 = 0.985	1686:1695	Best fit model was found to be Hixon-crowell's model (r2 = 0.995) followed by Weibull's model (r2 = 0.985).
34602414	5	56	theme	disintegrants	943:955	arg1	concentrations					906:919	various concentrations	898:919	various concentrations of filler binders and disintegrants	898:955	In this investigation Mebeverine hydrochloride an anti-muscarinic drug is studied for compression and release behavior using various concentrations of filler binders and disintegrants via rotatable central composite design (CCRD) option of design expert (software).
34602414	4	57	theme	acceptable	698:707	arg1	parameters					717:726	acceptable quality parameters	698:726	acceptable quality parameters and hence preferred over granulation method	698:770	Later process offer fewer processing steps and agreeable release profile with acceptable quality parameters and hence preferred over granulation method.
34602414	0	58	theme	in	133:134	arg1	kinetics					150:157	in vitro release kinetics	133:157	in vitro release kinetics	133:157	Formulation development of directly compressible mebevarine tablets using superdisintegrant: A way to investigate quality atributes, in vitro release kinetics and stability profile.
34602414	9	59	theme	model	1549:1553	arg1	methods					1582:1588	model dependent and in-dependent methods	1549:1588	model dependent and in-dependent methods	1549:1588	Dissolution comparisons were statistically analyzed by ANOVA and model dependent and in-dependent methods.
34602414	2	60	theme	different	301:309	arg1	powders					311:317	different powders	301:317	different powders	301:317	Compressibility behavior of different powders and mixtures of formulations and release pattern of any tablets are characteristic measures to define prerequisite quality attributes of any compressed formulations.
34602414	5	61	theme	various	898:904	arg1	concentrations					906:919	various concentrations	898:919	various concentrations of filler binders and disintegrants	898:955	In this investigation Mebeverine hydrochloride an anti-muscarinic drug is studied for compression and release behavior using various concentrations of filler binders and disintegrants via rotatable central composite design (CCRD) option of design expert (software).
34602414	6	62	dep	Avicel	1188:1193	arg1	Filler/binder					1200:1212	Filler/binder	1200:1212	Filler/binder	1200:1212	Nine formulations were developed from F1 to F9 with Crospovidone (superdisintegrant) as (X1) (-α=1.17% to ± α=6.83%) and microcrystalline cellulose (Avicel 102, Filler/binder) as X2 (-α = 29.82% to ± α = 65.18%).
34602414	0	63	theme	release	142:148	arg1	kinetics					150:157	in vitro release kinetics	133:157	in vitro release kinetics	133:157	Formulation development of directly compressible mebevarine tablets using superdisintegrant: A way to investigate quality atributes, in vitro release kinetics and stability profile.
34602414	1	64	theme	solid	207:211	arg1	form					225:228	a solid oral dosage form	205:228	a solid oral dosage form	205:228	In order for preparing a solid oral dosage form, tablet quality is of significant concern.
34602414	4	65	theme	agreeable	667:675	arg1	profile					685:691	agreeable release profile	667:691	agreeable release profile	667:691	Later process offer fewer processing steps and agreeable release profile with acceptable quality parameters and hence preferred over granulation method.
34602414	3	66	theme	direct	600:605	arg1	compression					607:617	direct compression	600:617	direct compression	600:617	There are basically two major methods that can be adopted for the preparation of tablets including granulation and direct compression.
34602414	6	67	theme	-α	1222:1223	arg1	%					1248:1248	-α = 29.82% to ± α = 65.18%	1222:1248	-α = 29.82% to ± α = 65.18%	1222:1248	Nine formulations were developed from F1 to F9 with Crospovidone (superdisintegrant) as (X1) (-α=1.17% to ± α=6.83%) and microcrystalline cellulose (Avicel 102, Filler/binder) as X2 (-α = 29.82% to ± α = 65.18%).
34602414	10	68	dep	model	1679:1683	arg1	r2					1686:1687	r2	1686:1687	r2 = 0.985	1686:1695	Best fit model was found to be Hixon-crowell's model (r2 = 0.995) followed by Weibull's model (r2 = 0.985).
34602414	3	69	theme	tablets	566:572	arg1	preparation					551:561	the preparation	547:561	the preparation of tablets including granulation and direct compression	547:617	There are basically two major methods that can be adopted for the preparation of tablets including granulation and direct compression.
34602414	8	70	theme	blends	1393:1398	arg1	performance					1371:1381	The performance	1367:1381	The performance of powder blends and formulations	1367:1415	The performance of powder blends and formulations was analyzed by micromeritic and physico-chemical and assessments.
34602414	11	71	theme	stability	1807:1815	arg1	tests					1817:1821	stability tests	1807:1821	stability tests	1807:1821	The Trial formulations F2, F4, F6 and F8 were also studied on accelerated conditions (40±5ºC 75%±5% RH) for stability tests and validity of the formulations in months were also determined between 35-39 months.
34602414	5	72	theme	rotatable	961:969	arg1	option					1003:1008	rotatable central composite design (CCRD) option	961:1008	rotatable central composite design (CCRD) option of design expert (software)	961:1036	In this investigation Mebeverine hydrochloride an anti-muscarinic drug is studied for compression and release behavior using various concentrations of filler binders and disintegrants via rotatable central composite design (CCRD) option of design expert (software).
34602414	8	73	theme	formulations	1404:1415	arg1	performance					1371:1381	The performance	1367:1381	The performance of powder blends and formulations	1367:1415	The performance of powder blends and formulations was analyzed by micromeritic and physico-chemical and assessments.
34602414	5	74	theme	anti-muscarinic	823:837	arg1	drug					839:842	an anti-muscarinic drug	820:842	an anti-muscarinic drug	820:842	In this investigation Mebeverine hydrochloride an anti-muscarinic drug is studied for compression and release behavior using various concentrations of filler binders and disintegrants via rotatable central composite design (CCRD) option of design expert (software).
34602414	0	75	dep	in	133:134	arg1	vitro					136:140	vitro	136:140	vitro	136:140	Formulation development of directly compressible mebevarine tablets using superdisintegrant: A way to investigate quality atributes, in vitro release kinetics and stability profile.
34602414	5	76	theme	software	1028:1035	arg1	option					1003:1008	rotatable central composite design (CCRD) option	961:1008	rotatable central composite design (CCRD) option of design expert (software)	961:1036	In this investigation Mebeverine hydrochloride an anti-muscarinic drug is studied for compression and release behavior using various concentrations of filler binders and disintegrants via rotatable central composite design (CCRD) option of design expert (software).
34602414	5	77	theme	investigation	781:793	arg1	hydrochloride					806:818	this investigation Mebeverine hydrochloride	776:818	this investigation Mebeverine hydrochloride	776:818	In this investigation Mebeverine hydrochloride an anti-muscarinic drug is studied for compression and release behavior using various concentrations of filler binders and disintegrants via rotatable central composite design (CCRD) option of design expert (software).
34602414	11	78	dep	formulations	1709:1720	arg1	F6					1730:1731	F6	1730:1731	F6	1730:1731	The Trial formulations F2, F4, F6 and F8 were also studied on accelerated conditions (40±5ºC 75%±5% RH) for stability tests and validity of the formulations in months were also determined between 35-39 months.
34602414	11	78	dep	formulations	1709:1720	arg1	formulations					1709:1720	The Trial formulations	1699:1720	The Trial formulations F2, F4, F6 and F8	1699:1738	The Trial formulations F2, F4, F6 and F8 were also studied on accelerated conditions (40±5ºC 75%±5% RH) for stability tests and validity of the formulations in months were also determined between 35-39 months.
34602414	11	78	dep	formulations	1709:1720	arg1	F2					1722:1723	F2	1722:1723	F2	1722:1723	The Trial formulations F2, F4, F6 and F8 were also studied on accelerated conditions (40±5ºC 75%±5% RH) for stability tests and validity of the formulations in months were also determined between 35-39 months.
34602414	11	78	dep	formulations	1709:1720	arg1	F8					1737:1738	F8	1737:1738	F8	1737:1738	The Trial formulations F2, F4, F6 and F8 were also studied on accelerated conditions (40±5ºC 75%±5% RH) for stability tests and validity of the formulations in months were also determined between 35-39 months.
34602414	10	79	theme	fit	1596:1598	arg1	model					1600:1604	Best fit model	1591:1604	Best fit model	1591:1604	Best fit model was found to be Hixon-crowell's model (r2 = 0.995) followed by Weibull's model (r2 = 0.985).
34602414	6	80	dep	as	1124:1125	arg1	%					1140:1140	-α=1.17%	1133:1140	-α=1.17% to ± α=6.83%	1133:1153	Nine formulations were developed from F1 to F9 with Crospovidone (superdisintegrant) as (X1) (-α=1.17% to ± α=6.83%) and microcrystalline cellulose (Avicel 102, Filler/binder) as X2 (-α = 29.82% to ± α = 65.18%).
34602414	2	81	theme	tablets	375:381	arg1	formulations					335:346	formulations	335:346	formulations	335:346	Compressibility behavior of different powders and mixtures of formulations and release pattern of any tablets are characteristic measures to define prerequisite quality attributes of any compressed formulations.
34602414	2	81	theme	tablets	375:381	arg1	pattern					360:366	release pattern	352:366	release pattern	352:366	Compressibility behavior of different powders and mixtures of formulations and release pattern of any tablets are characteristic measures to define prerequisite quality attributes of any compressed formulations.
34602414	0	82	dep	development	12:22	arg1	way					95:97	A way to investigate quality atributes	93:130	A way to investigate quality atributes	93:130	Formulation development of directly compressible mebevarine tablets using superdisintegrant: A way to investigate quality atributes, in vitro release kinetics and stability profile.
34602414	0	82	dep	development	12:22	arg1	kinetics					150:157	in vitro release kinetics	133:157	in vitro release kinetics	133:157	Formulation development of directly compressible mebevarine tablets using superdisintegrant: A way to investigate quality atributes, in vitro release kinetics and stability profile.
34602414	0	82	dep	development	12:22	arg1	profile					173:179	stability profile	163:179	stability profile	163:179	Formulation development of directly compressible mebevarine tablets using superdisintegrant: A way to investigate quality atributes, in vitro release kinetics and stability profile.
34602414	5	83	theme	expert	1020:1025	arg1	software					1028:1035	design expert (software)	1013:1036	design expert (software)	1013:1036	In this investigation Mebeverine hydrochloride an anti-muscarinic drug is studied for compression and release behavior using various concentrations of filler binders and disintegrants via rotatable central composite design (CCRD) option of design expert (software).
34602414	10	84	theme	Weibull	1669:1675	arg1	model					1679:1683	Weibull's model	1669:1683	Weibull's model (r2 = 0.985)	1669:1696	Best fit model was found to be Hixon-crowell's model (r2 = 0.995) followed by Weibull's model (r2 = 0.985).
34602414	2	85	theme	prerequisite	421:432	arg1	attributes					442:451	prerequisite quality attributes	421:451	prerequisite quality attributes of any compressed formulations	421:482	Compressibility behavior of different powders and mixtures of formulations and release pattern of any tablets are characteristic measures to define prerequisite quality attributes of any compressed formulations.
34602414	0	86	theme	mebevarine	49:58	arg1	tablets					60:66	directly compressible mebevarine tablets	27:66	directly compressible mebevarine tablets using superdisintegrant	27:90	Formulation development of directly compressible mebevarine tablets using superdisintegrant: A way to investigate quality atributes, in vitro release kinetics and stability profile.
34602414	7	87	theme	Disintegration	1252:1265	arg1	Hardness					1289:1296	Hardness	1289:1296	Hardness in (kg) as (R2)	1289:1312	Disintegration Time (DT) as (R1) and Hardness in (kg) as (R2) were determined as two dependent response variables.
34602414	7	87	theme	Disintegration	1252:1265	arg1	variables					1356:1364	two dependent response variables	1333:1364	two dependent response variables	1333:1364	Disintegration Time (DT) as (R1) and Hardness in (kg) as (R2) were determined as two dependent response variables.
34602414	7	87	theme	Disintegration	1252:1265	arg1	DT					1273:1274	DT	1273:1274	DT	1273:1274	Disintegration Time (DT) as (R1) and Hardness in (kg) as (R2) were determined as two dependent response variables.
34602414	7	87	theme	Disintegration	1252:1265	arg1	Time					1267:1270	Disintegration Time	1252:1270	Disintegration Time (DT) as (R1)	1252:1283	Disintegration Time (DT) as (R1) and Hardness in (kg) as (R2) were determined as two dependent response variables.
34602414	7	87	theme	Disintegration	1252:1265	arg1	R1					1281:1282	R1	1281:1282	R1	1281:1282	Disintegration Time (DT) as (R1) and Hardness in (kg) as (R2) were determined as two dependent response variables.
34602414	0	88	theme	quality	114:120	arg1	atributes					122:130	quality atributes	114:130	quality atributes	114:130	Formulation development of directly compressible mebevarine tablets using superdisintegrant: A way to investigate quality atributes, in vitro release kinetics and stability profile.
34602414	7	89	from	Time	1267:1270	arg1	R2					1310:1311	R2	1310:1311	R2	1310:1311	Disintegration Time (DT) as (R1) and Hardness in (kg) as (R2) were determined as two dependent response variables.
34602414	7	89	from	Time	1267:1270	arg1	as					1306:1307	as	1306:1307	as	1306:1307	Disintegration Time (DT) as (R1) and Hardness in (kg) as (R2) were determined as two dependent response variables.
34602414	2	90	theme	formulations	471:482	arg1	attributes					442:451	prerequisite quality attributes	421:451	prerequisite quality attributes of any compressed formulations	421:482	Compressibility behavior of different powders and mixtures of formulations and release pattern of any tablets are characteristic measures to define prerequisite quality attributes of any compressed formulations.
34602414	6	91	dep	%	1140:1140	arg1	to					1142:1143	to	1142:1143	to	1142:1143	Nine formulations were developed from F1 to F9 with Crospovidone (superdisintegrant) as (X1) (-α=1.17% to ± α=6.83%) and microcrystalline cellulose (Avicel 102, Filler/binder) as X2 (-α = 29.82% to ± α = 65.18%).
34602414	6	91	dep	%	1140:1140	arg1	%					1153:1153	± α=6.83%	1145:1153	-α=1.17% to ± α=6.83%	1133:1153	Nine formulations were developed from F1 to F9 with Crospovidone (superdisintegrant) as (X1) (-α=1.17% to ± α=6.83%) and microcrystalline cellulose (Avicel 102, Filler/binder) as X2 (-α = 29.82% to ± α = 65.18%).
34602414	2	92	theme	formulations	335:346	arg1	mixtures					323:330	mixtures	323:330	mixtures of formulations and release pattern of any tablets	323:381	Compressibility behavior of different powders and mixtures of formulations and release pattern of any tablets are characteristic measures to define prerequisite quality attributes of any compressed formulations.
34602414	2	92	theme	formulations	335:346	arg1	behavior					289:296	Compressibility behavior	273:296	Compressibility behavior of different powders	273:317	Compressibility behavior of different powders and mixtures of formulations and release pattern of any tablets are characteristic measures to define prerequisite quality attributes of any compressed formulations.
34602414	9	93	theme	dependent	1555:1563	arg1	methods					1582:1588	model dependent and in-dependent methods	1549:1588	model dependent and in-dependent methods	1549:1588	Dissolution comparisons were statistically analyzed by ANOVA and model dependent and in-dependent methods.
34602414	1	94	theme	oral	213:216	arg1	form					225:228	a solid oral dosage form	205:228	a solid oral dosage form	205:228	In order for preparing a solid oral dosage form, tablet quality is of significant concern.
34602414	9	95	theme	in-dependent	1569:1580	arg1	methods					1582:1588	model dependent and in-dependent methods	1549:1588	model dependent and in-dependent methods	1549:1588	Dissolution comparisons were statistically analyzed by ANOVA and model dependent and in-dependent methods.
34602414	10	96	theme	=	1648:1648	arg1	model					1638:1642	Hixon-crowell's model	1622:1642	Hixon-crowell's model (r2 = 0.995) followed by Weibull's model (r2 = 0.985)	1622:1696	Best fit model was found to be Hixon-crowell's model (r2 = 0.995) followed by Weibull's model (r2 = 0.985).
34602414	10	96	theme	=	1648:1648	arg1	0.995					1650:1654	r2 = 0.995	1645:1654	r2 = 0.995	1645:1654	Best fit model was found to be Hixon-crowell's model (r2 = 0.995) followed by Weibull's model (r2 = 0.985).
34602414	4	97	theme	processing	646:655	arg1	steps					657:661	fewer processing steps	640:661	fewer processing steps	640:661	Later process offer fewer processing steps and agreeable release profile with acceptable quality parameters and hence preferred over granulation method.
34602414	2	98	dep	characteristic	387:400	arg1	define					414:419	define	414:419	to define prerequisite quality attributes of any compressed formulations	411:482	Compressibility behavior of different powders and mixtures of formulations and release pattern of any tablets are characteristic measures to define prerequisite quality attributes of any compressed formulations.
34602414	1	99	theme	tablet	231:236	arg1	quality					238:244	tablet quality	231:244	tablet quality	231:244	In order for preparing a solid oral dosage form, tablet quality is of significant concern.
32081758	1	0	theme	natural	143:149	arg1	macromolecule					162:174	a natural biological macromolecule	141:174	a natural biological macromolecule with several potential biomedical applications	141:221	Arabinoxylan (AX) is a natural biological macromolecule with several potential biomedical applications.
32081758	1	0	theme	natural	143:149	arg1	Arabinoxylan					120:131	Arabinoxylan	120:131	Arabinoxylan (AX)	120:136	Arabinoxylan (AX) is a natural biological macromolecule with several potential biomedical applications.
32081758	7	1	theme	mechanical	970:979	arg1	strength					981:988	substantial mechanical strength	958:988	substantial mechanical strength	958:988	PNS3 proved more biocompatible, showed interconnected porosity and substantial mechanical strength compared to PNS1, PNS2 and PNS4.
32081758	8	2	theme	cell	1082:1085	arg1	growth					1087:1092	cell growth	1082:1092	cell growth	1082:1092	Furthermore, it has also showed more affinity to cells and cell growth.
32081758	1	3	theme	biological	151:160	arg1	macromolecule					162:174	a natural biological macromolecule	141:174	a natural biological macromolecule with several potential biomedical applications	141:221	Arabinoxylan (AX) is a natural biological macromolecule with several potential biomedical applications.
32081758	1	3	theme	biological	151:160	arg1	Arabinoxylan					120:131	Arabinoxylan	120:131	Arabinoxylan (AX)	120:136	Arabinoxylan (AX) is a natural biological macromolecule with several potential biomedical applications.
32081758	4	4	theme	porosity	613:620	arg1	morphology					640:649	good porosity and rough surface morphology	608:649	good porosity and rough surface morphology	608:649	The scaffolds exhibited good porosity and rough surface morphology, which were efficiently controlled by TiO2 concentrations.
32081758	5	5	theme	scaffolds	780:788	arg1	biocompatibility					754:769	the biocompatibility	750:769	the biocompatibility of these scaffolds	750:788	MC3T3-E1 cells were employed to conduct the biocompatibility of these scaffolds.
32081758	5	6	theme	MC3T3-E1	710:717	arg1	cells					719:723	MC3T3-E1 cells	710:723	MC3T3-E1 cells	710:723	MC3T3-E1 cells were employed to conduct the biocompatibility of these scaffolds.
32081758	3	7	theme	physicochemical	391:405	arg1	characterizations					407:423	The physicochemical characterizations	387:423	The physicochemical characterizations of these polymeric nanocomposite scaffolds	387:466	The physicochemical characterizations of these polymeric nanocomposite scaffolds were performed for surface morphology, porosity, swelling, biodegradability, mechanical, and biological properties.
32081758	4	8	theme	surface	632:638	arg1	morphology					640:649	good porosity and rough surface morphology	608:649	good porosity and rough surface morphology	608:649	The scaffolds exhibited good porosity and rough surface morphology, which were efficiently controlled by TiO2 concentrations.
32081758	0	9	theme	in	104:105	arg1	study					113:117	An in vitro study	101:117	Arabinoxylan-co-AA/HAp/TiO2 nanocomposite scaffold a potential material for bone tissue engineering: An in vitro study.	0:118	Arabinoxylan-co-AA/HAp/TiO2 nanocomposite scaffold a potential material for bone tissue engineering: An in vitro study.
32081758	8	10	theme	more	1055:1058	arg1	affinity					1060:1067	more affinity	1055:1067	more affinity	1055:1067	Furthermore, it has also showed more affinity to cells and cell growth.
32081758	2	11	theme	titanium	278:285	arg1	TiO2					296:299	TiO2	296:299	TiO2	296:299	In this research, AX, nano-hydroxyapatite (n-HAp) and titanium dioxide (TiO2) based polymeric nanocomposite scaffolds were fabricated by the freeze-drying method.
32081758	2	11	theme	titanium	278:285	arg1	dioxide					287:293	titanium dioxide	278:293	titanium dioxide (TiO2)	278:300	In this research, AX, nano-hydroxyapatite (n-HAp) and titanium dioxide (TiO2) based polymeric nanocomposite scaffolds were fabricated by the freeze-drying method.
32081758	9	12	theme	bioactive	1128:1136	arg1	scaffold					1152:1159	the bioactive nanocomposite scaffold	1124:1159	the bioactive nanocomposite scaffold	1124:1159	The results illustrated that the bioactive nanocomposite scaffold has the potential to find applications in the tissue engineering field.
32081758	4	13	theme	rough	626:630	arg1	morphology					640:649	good porosity and rough surface morphology	608:649	good porosity and rough surface morphology	608:649	The scaffolds exhibited good porosity and rough surface morphology, which were efficiently controlled by TiO2 concentrations.
32081758	9	14	theme	tissue	1207:1212	arg1	field					1226:1230	the tissue engineering field	1203:1230	the tissue engineering field	1203:1230	The results illustrated that the bioactive nanocomposite scaffold has the potential to find applications in the tissue engineering field.
32081758	1	15	dep	Arabinoxylan	120:131	arg1	AX					134:135	AX	134:135	AX	134:135	Arabinoxylan (AX) is a natural biological macromolecule with several potential biomedical applications.
32081758	3	16	theme	nanocomposite	444:456	arg1	scaffolds					458:466	these polymeric nanocomposite scaffolds	428:466	these polymeric nanocomposite scaffolds	428:466	The physicochemical characterizations of these polymeric nanocomposite scaffolds were performed for surface morphology, porosity, swelling, biodegradability, mechanical, and biological properties.
32081758	0	17	dep	in	104:105	arg1	vitro					107:111	vitro	107:111	vitro	107:111	Arabinoxylan-co-AA/HAp/TiO2 nanocomposite scaffold a potential material for bone tissue engineering: An in vitro study.
32081758	0	18	theme	nanocomposite	28:40	arg1	material					63:70	Arabinoxylan-co-AA/HAp/TiO2 nanocomposite scaffold a potential material	0:70	Arabinoxylan-co-AA/HAp/TiO2 nanocomposite scaffold a potential material for bone tissue engineering: An in vitro study.	0:118	Arabinoxylan-co-AA/HAp/TiO2 nanocomposite scaffold a potential material for bone tissue engineering: An in vitro study.
32081758	2	19	theme	based	302:306	arg1	scaffolds					332:340	based polymeric nanocomposite scaffolds	302:340	based polymeric nanocomposite scaffolds	302:340	In this research, AX, nano-hydroxyapatite (n-HAp) and titanium dioxide (TiO2) based polymeric nanocomposite scaffolds were fabricated by the freeze-drying method.
32081758	1	20	theme	several	181:187	arg1	applications					210:221	several potential biomedical applications	181:221	several potential biomedical applications	181:221	Arabinoxylan (AX) is a natural biological macromolecule with several potential biomedical applications.
32081758	0	21	theme	Arabinoxylan-co-AA/HAp/TiO2	0:26	arg1	material					63:70	Arabinoxylan-co-AA/HAp/TiO2 nanocomposite scaffold a potential material	0:70	Arabinoxylan-co-AA/HAp/TiO2 nanocomposite scaffold a potential material for bone tissue engineering: An in vitro study.	0:118	Arabinoxylan-co-AA/HAp/TiO2 nanocomposite scaffold a potential material for bone tissue engineering: An in vitro study.
32081758	1	22	theme	potential	189:197	arg1	applications					210:221	several potential biomedical applications	181:221	several potential biomedical applications	181:221	Arabinoxylan (AX) is a natural biological macromolecule with several potential biomedical applications.
32081758	0	23	theme	a	51:51	arg1	material					63:70	Arabinoxylan-co-AA/HAp/TiO2 nanocomposite scaffold a potential material	0:70	Arabinoxylan-co-AA/HAp/TiO2 nanocomposite scaffold a potential material for bone tissue engineering: An in vitro study.	0:118	Arabinoxylan-co-AA/HAp/TiO2 nanocomposite scaffold a potential material for bone tissue engineering: An in vitro study.
32081758	6	24	dep	cell	863:866	arg1	attachment					868:877	attachment	868:877	attachment	868:877	Scaffolds showed unique biocompatibility in vitro and was favorable for cell attachment and growth.
32081758	1	25	theme	biomedical	199:208	arg1	applications					210:221	several potential biomedical applications	181:221	several potential biomedical applications	181:221	Arabinoxylan (AX) is a natural biological macromolecule with several potential biomedical applications.
32081758	0	26	theme	scaffold	42:49	arg1	material					63:70	Arabinoxylan-co-AA/HAp/TiO2 nanocomposite scaffold a potential material	0:70	Arabinoxylan-co-AA/HAp/TiO2 nanocomposite scaffold a potential material for bone tissue engineering: An in vitro study.	0:118	Arabinoxylan-co-AA/HAp/TiO2 nanocomposite scaffold a potential material for bone tissue engineering: An in vitro study.
32081758	4	27	theme	good	608:611	arg1	morphology					640:649	good porosity and rough surface morphology	608:649	good porosity and rough surface morphology	608:649	The scaffolds exhibited good porosity and rough surface morphology, which were efficiently controlled by TiO2 concentrations.
32081758	7	28	theme	interconnected	930:943	arg1	porosity					945:952	interconnected porosity	930:952	interconnected porosity	930:952	PNS3 proved more biocompatible, showed interconnected porosity and substantial mechanical strength compared to PNS1, PNS2 and PNS4.
32081758	4	29	theme	TiO2	689:692	arg1	concentrations					694:707	TiO2 concentrations	689:707	TiO2 concentrations	689:707	The scaffolds exhibited good porosity and rough surface morphology, which were efficiently controlled by TiO2 concentrations.
32081758	3	30	theme	scaffolds	458:466	arg1	characterizations					407:423	The physicochemical characterizations	387:423	The physicochemical characterizations of these polymeric nanocomposite scaffolds	387:466	The physicochemical characterizations of these polymeric nanocomposite scaffolds were performed for surface morphology, porosity, swelling, biodegradability, mechanical, and biological properties.
32081758	2	31	dep	AX	242:243	arg1	scaffolds					332:340	based polymeric nanocomposite scaffolds	302:340	based polymeric nanocomposite scaffolds	302:340	In this research, AX, nano-hydroxyapatite (n-HAp) and titanium dioxide (TiO2) based polymeric nanocomposite scaffolds were fabricated by the freeze-drying method.
32081758	0	32	theme	potential	53:61	arg1	material					63:70	Arabinoxylan-co-AA/HAp/TiO2 nanocomposite scaffold a potential material	0:70	Arabinoxylan-co-AA/HAp/TiO2 nanocomposite scaffold a potential material for bone tissue engineering: An in vitro study.	0:118	Arabinoxylan-co-AA/HAp/TiO2 nanocomposite scaffold a potential material for bone tissue engineering: An in vitro study.
32081758	3	33	theme	polymeric	434:442	arg1	scaffolds					458:466	these polymeric nanocomposite scaffolds	428:466	these polymeric nanocomposite scaffolds	428:466	The physicochemical characterizations of these polymeric nanocomposite scaffolds were performed for surface morphology, porosity, swelling, biodegradability, mechanical, and biological properties.
32081758	3	34	theme	surface	487:493	arg1	morphology					495:504	surface morphology	487:504	surface morphology	487:504	The physicochemical characterizations of these polymeric nanocomposite scaffolds were performed for surface morphology, porosity, swelling, biodegradability, mechanical, and biological properties.
32081758	0	35	theme	bone	76:79	arg1	engineering					88:98	bone tissue engineering	76:98	bone tissue engineering	76:98	Arabinoxylan-co-AA/HAp/TiO2 nanocomposite scaffold a potential material for bone tissue engineering: An in vitro study.
32081758	0	36	dep	material	63:70	arg1	study					113:117	An in vitro study	101:117	Arabinoxylan-co-AA/HAp/TiO2 nanocomposite scaffold a potential material for bone tissue engineering: An in vitro study.	0:118	Arabinoxylan-co-AA/HAp/TiO2 nanocomposite scaffold a potential material for bone tissue engineering: An in vitro study.
32081758	9	37	from	applications	1187:1198	arg1	field					1226:1230	the tissue engineering field	1203:1230	the tissue engineering field	1203:1230	The results illustrated that the bioactive nanocomposite scaffold has the potential to find applications in the tissue engineering field.
32081758	9	38	theme	nanocomposite	1138:1150	arg1	scaffold					1152:1159	the bioactive nanocomposite scaffold	1124:1159	the bioactive nanocomposite scaffold	1124:1159	The results illustrated that the bioactive nanocomposite scaffold has the potential to find applications in the tissue engineering field.
32081758	1	39	with	macromolecule	162:174	arg1	applications					210:221	several potential biomedical applications	181:221	several potential biomedical applications	181:221	Arabinoxylan (AX) is a natural biological macromolecule with several potential biomedical applications.
32081758	6	40	theme	unique	808:813	arg1	biocompatibility					815:830	unique biocompatibility	808:830	unique biocompatibility	808:830	Scaffolds showed unique biocompatibility in vitro and was favorable for cell attachment and growth.
32081758	2	41	theme	nanocomposite	318:330	arg1	scaffolds					332:340	based polymeric nanocomposite scaffolds	302:340	based polymeric nanocomposite scaffolds	302:340	In this research, AX, nano-hydroxyapatite (n-HAp) and titanium dioxide (TiO2) based polymeric nanocomposite scaffolds were fabricated by the freeze-drying method.
32081758	3	42	theme	biological	561:570	arg1	properties					572:581	biological properties	561:581	biological properties	561:581	The physicochemical characterizations of these polymeric nanocomposite scaffolds were performed for surface morphology, porosity, swelling, biodegradability, mechanical, and biological properties.
32081758	9	43	theme	engineering	1214:1224	arg1	field					1226:1230	the tissue engineering field	1203:1230	the tissue engineering field	1203:1230	The results illustrated that the bioactive nanocomposite scaffold has the potential to find applications in the tissue engineering field.
32081758	2	44	theme	polymeric	308:316	arg1	scaffolds					332:340	based polymeric nanocomposite scaffolds	302:340	based polymeric nanocomposite scaffolds	302:340	In this research, AX, nano-hydroxyapatite (n-HAp) and titanium dioxide (TiO2) based polymeric nanocomposite scaffolds were fabricated by the freeze-drying method.
32081758	2	45	theme	freeze-drying	365:377	arg1	method					379:384	the freeze-drying method	361:384	the freeze-drying method	361:384	In this research, AX, nano-hydroxyapatite (n-HAp) and titanium dioxide (TiO2) based polymeric nanocomposite scaffolds were fabricated by the freeze-drying method.
32081758	0	46	theme	tissue	81:86	arg1	engineering					88:98	bone tissue engineering	76:98	bone tissue engineering	76:98	Arabinoxylan-co-AA/HAp/TiO2 nanocomposite scaffold a potential material for bone tissue engineering: An in vitro study.
32081758	9	47	contain	has	1161:1163	arg1	scaffold					1152:1159	the bioactive nanocomposite scaffold	1124:1159	the bioactive nanocomposite scaffold	1124:1159	The results illustrated that the bioactive nanocomposite scaffold has the potential to find applications in the tissue engineering field.
32081758	9	47	contain	has	1161:1163	arg2	potential					1169:1177	the potential to find applications in the tissue engineering field	1165:1230	the potential to find applications in the tissue engineering field	1165:1230	The results illustrated that the bioactive nanocomposite scaffold has the potential to find applications in the tissue engineering field.
32081758	7	48	dep	proved	896:901	arg1	showed					923:928	showed	923:928	showed interconnected porosity and substantial mechanical strength compared to PNS1, PNS2 and PNS4	923:1020	PNS3 proved more biocompatible, showed interconnected porosity and substantial mechanical strength compared to PNS1, PNS2 and PNS4.
32081758	7	49	theme	substantial	958:968	arg1	strength					981:988	substantial mechanical strength	958:988	substantial mechanical strength	958:988	PNS3 proved more biocompatible, showed interconnected porosity and substantial mechanical strength compared to PNS1, PNS2 and PNS4.
32341352	6	0	theme	biorecognition	1190:1203	arg1	motifs					1205:1210	biorecognition motifs	1190:1210	biorecognition motifs	1190:1210	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	6	1	theme	nano-enabled	1335:1346	arg1	agriculture					1348:1358	nano-enabled agriculture	1335:1358	nano-enabled agriculture	1335:1358	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	3	2	theme	low	513:515	arg1	window					528:533	a low background window	511:533	a low background window	511:533	Quantum dot (QD) fluorescence emission in a low background window allows confocal microscopy imaging and quantitative detection by elemental analysis in plant cells and organelles.
32341352	6	3	with	nanomaterials	1144:1156	arg1	cargoes					1172:1178	chemical cargoes	1163:1178	chemical cargoes guided by biorecognition motifs	1163:1210	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	6	4	theme	Targeted	1123:1130	arg1	delivery					1132:1139	Targeted delivery	1123:1139	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs	1123:1210	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	6	5	from	interactions	1317:1328	arg1	bioengineering					1282:1295	bioengineering	1282:1295	bioengineering	1282:1295	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	6	5	from	interactions	1317:1328	arg1	biology					1270:1276	plant biology	1264:1276	plant biology	1264:1276	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	3	6	theme	background	517:526	arg1	window					528:533	a low background window	511:533	a low background window	511:533	Quantum dot (QD) fluorescence emission in a low background window allows confocal microscopy imaging and quantitative detection by elemental analysis in plant cells and organelles.
32341352	5	7	theme	chemical	966:973	arg1	cargoes					975:981	chemical cargoes	966:981	chemical cargoes	966:981	Peptide biorecognition provides high delivery efficiency and specificity of QD with chemical cargoes to chloroplasts in plant cells in vivo (74.6 ± 10.8%) and more specific tunable changes of chloroplast redox function than chemicals alone.
32341352	6	8	from	range	1224:1228	arg1	bioengineering					1282:1295	bioengineering	1282:1295	bioengineering	1282:1295	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	6	8	from	range	1224:1228	arg1	biology					1270:1276	plant biology	1264:1276	plant biology	1264:1276	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	3	9	from	emission	499:506	arg1	window					528:533	a low background window	511:533	a low background window	511:533	Quantum dot (QD) fluorescence emission in a low background window allows confocal microscopy imaging and quantitative detection by elemental analysis in plant cells and organelles.
32341352	6	10	theme	applications	1248:1259	arg1	agriculture					1348:1358	nano-enabled agriculture	1335:1358	nano-enabled agriculture	1335:1358	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	6	10	theme	applications	1248:1259	arg1	range					1224:1228	a broad range	1216:1228	a broad range of nanotechnology applications in plant biology and bioengineering	1216:1295	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	6	10	theme	applications	1248:1259	arg1	interactions					1317:1328	nanoparticle-plant interactions	1298:1328	nanoparticle-plant interactions	1298:1328	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	4	11	theme	conserved	816:824	arg1	peptide					834:840	a rationally designed and conserved guiding peptide	790:840	a rationally designed and conserved guiding peptide	790:840	QD functionalization with β-cyclodextrin molecular baskets enables loading and delivery of diverse chemicals, and nanoparticle coating with a rationally designed and conserved guiding peptide targets their delivery to chloroplasts.
32341352	2	12	theme	nanoscale	299:307	arg1	platform					309:316	a nanoscale platform	297:316	a nanoscale platform that targets and delivers nanomaterials with biochemicals to plant photosynthetic organelles (chloroplasts) using a guiding peptide recognition motif	297:466	We demonstrate a nanoscale platform that targets and delivers nanomaterials with biochemicals to plant photosynthetic organelles (chloroplasts) using a guiding peptide recognition motif.
32341352	1	13	theme	plant	266:270	arg1	function					272:279	plant function	266:279	plant function	266:279	Current approaches for nanomaterial delivery in plants are unable to target specific subcellular compartments with high precision, limiting our ability to engineer plant function.
32341352	0	14	theme	biorecognition	80:93	arg1	motif					95:99	a biorecognition motif	78:99	a biorecognition motif	78:99	Targeted delivery of nanomaterials with chemical cargoes in plants enabled by a biorecognition motif.
32341352	6	15	theme	plant	1264:1268	arg1	biology					1270:1276	plant biology	1264:1276	plant biology	1264:1276	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	4	16	theme	chemicals	749:757	arg1	delivery					729:736	delivery	729:736	delivery	729:736	QD functionalization with β-cyclodextrin molecular baskets enables loading and delivery of diverse chemicals, and nanoparticle coating with a rationally designed and conserved guiding peptide targets their delivery to chloroplasts.
32341352	4	16	theme	chemicals	749:757	arg1	loading					717:723	loading	717:723	loading	717:723	QD functionalization with β-cyclodextrin molecular baskets enables loading and delivery of diverse chemicals, and nanoparticle coating with a rationally designed and conserved guiding peptide targets their delivery to chloroplasts.
32341352	4	17	theme	molecular	691:699	arg1	baskets					701:707	β-cyclodextrin molecular baskets	676:707	β-cyclodextrin molecular baskets	676:707	QD functionalization with β-cyclodextrin molecular baskets enables loading and delivery of diverse chemicals, and nanoparticle coating with a rationally designed and conserved guiding peptide targets their delivery to chloroplasts.
32341352	4	18	theme	diverse	741:747	arg1	chemicals					749:757	diverse chemicals	741:757	diverse chemicals	741:757	QD functionalization with β-cyclodextrin molecular baskets enables loading and delivery of diverse chemicals, and nanoparticle coating with a rationally designed and conserved guiding peptide targets their delivery to chloroplasts.
32341352	0	19	from	cargoes	49:55	arg1	plants					60:65	plants	60:65	plants	60:65	Targeted delivery of nanomaterials with chemical cargoes in plants enabled by a biorecognition motif.
32341352	5	20	theme	high	914:917	arg1	efficiency					928:937	high delivery efficiency	914:937	high delivery efficiency	914:937	Peptide biorecognition provides high delivery efficiency and specificity of QD with chemical cargoes to chloroplasts in plant cells in vivo (74.6 ± 10.8%) and more specific tunable changes of chloroplast redox function than chemicals alone.
32341352	5	20	theme	high	914:917	arg1	%					1034:1034	74.6 ± 10.8%	1023:1034	74.6 ± 10.8%	1023:1034	Peptide biorecognition provides high delivery efficiency and specificity of QD with chemical cargoes to chloroplasts in plant cells in vivo (74.6 ± 10.8%) and more specific tunable changes of chloroplast redox function than chemicals alone.
32341352	3	21	theme	confocal	542:549	arg1	microscopy					551:560	confocal microscopy	542:560	confocal microscopy imaging	542:568	Quantum dot (QD) fluorescence emission in a low background window allows confocal microscopy imaging and quantitative detection by elemental analysis in plant cells and organelles.
32341352	5	22	theme	plant	1002:1006	arg1	cells					1008:1012	plant cells	1002:1012	plant cells	1002:1012	Peptide biorecognition provides high delivery efficiency and specificity of QD with chemical cargoes to chloroplasts in plant cells in vivo (74.6 ± 10.8%) and more specific tunable changes of chloroplast redox function than chemicals alone.
32341352	3	23	theme	microscopy	551:560	arg1	imaging					562:568	confocal microscopy imaging	542:568	confocal microscopy imaging	542:568	Quantum dot (QD) fluorescence emission in a low background window allows confocal microscopy imaging and quantitative detection by elemental analysis in plant cells and organelles.
32341352	6	24	theme	chemical	1163:1170	arg1	cargoes					1172:1178	chemical cargoes	1163:1178	chemical cargoes guided by biorecognition motifs	1163:1210	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	3	25	theme	Quantum	469:475	arg1	emission					499:506	Quantum dot (QD) fluorescence emission	469:506	Quantum dot (QD) fluorescence emission in a low background window	469:533	Quantum dot (QD) fluorescence emission in a low background window allows confocal microscopy imaging and quantitative detection by elemental analysis in plant cells and organelles.
32341352	1	26	from	delivery	138:145	arg1	plants					150:155	plants	150:155	plants	150:155	Current approaches for nanomaterial delivery in plants are unable to target specific subcellular compartments with high precision, limiting our ability to engineer plant function.
32341352	6	27	theme	nanotechnology	1233:1246	arg1	applications					1248:1259	nanotechnology applications	1233:1259	nanotechnology applications in plant biology and bioengineering	1233:1295	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	1	28	theme	specific	178:185	arg1	compartments					199:210	specific subcellular compartments	178:210	specific subcellular compartments	178:210	Current approaches for nanomaterial delivery in plants are unable to target specific subcellular compartments with high precision, limiting our ability to engineer plant function.
32341352	0	29	theme	Targeted	0:7	arg1	delivery					9:16	Targeted delivery	0:16	Targeted delivery of nanomaterials with chemical cargoes in plants	0:65	Targeted delivery of nanomaterials with chemical cargoes in plants enabled by a biorecognition motif.
32341352	5	30	from	chloroplasts	986:997	arg1	cells					1008:1012	plant cells	1002:1012	plant cells	1002:1012	Peptide biorecognition provides high delivery efficiency and specificity of QD with chemical cargoes to chloroplasts in plant cells in vivo (74.6 ± 10.8%) and more specific tunable changes of chloroplast redox function than chemicals alone.
32341352	3	31	theme	elemental	600:608	arg1	analysis					610:617	elemental analysis	600:617	elemental analysis in plant cells and organelles	600:647	Quantum dot (QD) fluorescence emission in a low background window allows confocal microscopy imaging and quantitative detection by elemental analysis in plant cells and organelles.
32341352	3	32	from	analysis	610:617	arg1	organelles					638:647	organelles	638:647	organelles	638:647	Quantum dot (QD) fluorescence emission in a low background window allows confocal microscopy imaging and quantitative detection by elemental analysis in plant cells and organelles.
32341352	3	32	from	analysis	610:617	arg1	cells					628:632	plant cells	622:632	plant cells	622:632	Quantum dot (QD) fluorescence emission in a low background window allows confocal microscopy imaging and quantitative detection by elemental analysis in plant cells and organelles.
32341352	2	33	theme	organelles	400:409	arg1	chloroplasts					412:423	plant photosynthetic organelles (chloroplasts)	379:424	plant photosynthetic organelles (chloroplasts) using a guiding peptide recognition motif	379:466	We demonstrate a nanoscale platform that targets and delivers nanomaterials with biochemicals to plant photosynthetic organelles (chloroplasts) using a guiding peptide recognition motif.
32341352	0	34	theme	nanomaterials	21:33	arg1	delivery					9:16	Targeted delivery	0:16	Targeted delivery of nanomaterials with chemical cargoes in plants	0:65	Targeted delivery of nanomaterials with chemical cargoes in plants enabled by a biorecognition motif.
32341352	5	35	theme	delivery	919:926	arg1	efficiency					928:937	high delivery efficiency	914:937	high delivery efficiency	914:937	Peptide biorecognition provides high delivery efficiency and specificity of QD with chemical cargoes to chloroplasts in plant cells in vivo (74.6 ± 10.8%) and more specific tunable changes of chloroplast redox function than chemicals alone.
32341352	5	35	theme	delivery	919:926	arg1	%					1034:1034	74.6 ± 10.8%	1023:1034	74.6 ± 10.8%	1023:1034	Peptide biorecognition provides high delivery efficiency and specificity of QD with chemical cargoes to chloroplasts in plant cells in vivo (74.6 ± 10.8%) and more specific tunable changes of chloroplast redox function than chemicals alone.
32341352	6	36	theme	broad	1218:1222	arg1	range					1224:1228	a broad range	1216:1228	a broad range of nanotechnology applications in plant biology and bioengineering	1216:1295	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	3	37	theme	quantitative	574:585	arg1	detection					587:595	quantitative detection	574:595	quantitative detection	574:595	Quantum dot (QD) fluorescence emission in a low background window allows confocal microscopy imaging and quantitative detection by elemental analysis in plant cells and organelles.
32341352	5	38	theme	chloroplast	1074:1084	arg1	function					1092:1099	chloroplast redox function	1074:1099	chloroplast redox function	1074:1099	Peptide biorecognition provides high delivery efficiency and specificity of QD with chemical cargoes to chloroplasts in plant cells in vivo (74.6 ± 10.8%) and more specific tunable changes of chloroplast redox function than chemicals alone.
32341352	2	39	theme	peptide	442:448	arg1	motif					462:466	a guiding peptide recognition motif	432:466	a guiding peptide recognition motif	432:466	We demonstrate a nanoscale platform that targets and delivers nanomaterials with biochemicals to plant photosynthetic organelles (chloroplasts) using a guiding peptide recognition motif.
32341352	2	40	theme	recognition	450:460	arg1	motif					462:466	a guiding peptide recognition motif	432:466	a guiding peptide recognition motif	432:466	We demonstrate a nanoscale platform that targets and delivers nanomaterials with biochemicals to plant photosynthetic organelles (chloroplasts) using a guiding peptide recognition motif.
32341352	1	41	theme	Current	102:108	arg1	approaches					110:119	Current approaches	102:119	Current approaches for nanomaterial delivery in plants	102:155	Current approaches for nanomaterial delivery in plants are unable to target specific subcellular compartments with high precision, limiting our ability to engineer plant function.
32341352	2	42	theme	guiding	434:440	arg1	motif					462:466	a guiding peptide recognition motif	432:466	a guiding peptide recognition motif	432:466	We demonstrate a nanoscale platform that targets and delivers nanomaterials with biochemicals to plant photosynthetic organelles (chloroplasts) using a guiding peptide recognition motif.
32341352	0	43	theme	chemical	40:47	arg1	cargoes					49:55	chemical cargoes	40:55	chemical cargoes in plants	40:65	Targeted delivery of nanomaterials with chemical cargoes in plants enabled by a biorecognition motif.
32341352	6	44	from	applications	1248:1259	arg1	bioengineering					1282:1295	bioengineering	1282:1295	bioengineering	1282:1295	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	6	44	from	applications	1248:1259	arg1	biology					1270:1276	plant biology	1264:1276	plant biology	1264:1276	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	3	45	theme	dot	477:479	arg1	emission					499:506	Quantum dot (QD) fluorescence emission	469:506	Quantum dot (QD) fluorescence emission in a low background window	469:533	Quantum dot (QD) fluorescence emission in a low background window allows confocal microscopy imaging and quantitative detection by elemental analysis in plant cells and organelles.
32341352	3	46	theme	plant	622:626	arg1	cells					628:632	plant cells	622:632	plant cells	622:632	Quantum dot (QD) fluorescence emission in a low background window allows confocal microscopy imaging and quantitative detection by elemental analysis in plant cells and organelles.
32341352	1	47	theme	subcellular	187:197	arg1	compartments					199:210	specific subcellular compartments	178:210	specific subcellular compartments	178:210	Current approaches for nanomaterial delivery in plants are unable to target specific subcellular compartments with high precision, limiting our ability to engineer plant function.
32341352	1	48	theme	high	217:220	arg1	precision					222:230	high precision	217:230	high precision	217:230	Current approaches for nanomaterial delivery in plants are unable to target specific subcellular compartments with high precision, limiting our ability to engineer plant function.
32341352	6	49	from	agriculture	1348:1358	arg1	bioengineering					1282:1295	bioengineering	1282:1295	bioengineering	1282:1295	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	6	49	from	agriculture	1348:1358	arg1	biology					1270:1276	plant biology	1264:1276	plant biology	1264:1276	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	6	50	contain	has	1212:1214	arg2	agriculture					1348:1358	nano-enabled agriculture	1335:1358	nano-enabled agriculture	1335:1358	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	6	50	contain	has	1212:1214	arg2	interactions					1317:1328	nanoparticle-plant interactions	1298:1328	nanoparticle-plant interactions	1298:1328	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	6	50	contain	has	1212:1214	arg1	delivery					1132:1139	Targeted delivery	1123:1139	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs	1123:1210	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	6	50	contain	has	1212:1214	arg2	range					1224:1228	a broad range	1216:1228	a broad range of nanotechnology applications in plant biology and bioengineering	1216:1295	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	5	51	theme	redox	1086:1090	arg1	function					1092:1099	chloroplast redox function	1074:1099	chloroplast redox function	1074:1099	Peptide biorecognition provides high delivery efficiency and specificity of QD with chemical cargoes to chloroplasts in plant cells in vivo (74.6 ± 10.8%) and more specific tunable changes of chloroplast redox function than chemicals alone.
32341352	0	52	with	nanomaterials	21:33	arg1	cargoes					49:55	chemical cargoes	40:55	chemical cargoes in plants	40:65	Targeted delivery of nanomaterials with chemical cargoes in plants enabled by a biorecognition motif.
32341352	4	53	theme	β-cyclodextrin	676:689	arg1	baskets					701:707	β-cyclodextrin molecular baskets	676:707	β-cyclodextrin molecular baskets	676:707	QD functionalization with β-cyclodextrin molecular baskets enables loading and delivery of diverse chemicals, and nanoparticle coating with a rationally designed and conserved guiding peptide targets their delivery to chloroplasts.
32341352	2	54	with	nanomaterials	344:356	arg1	biochemicals					363:374	biochemicals	363:374	biochemicals	363:374	We demonstrate a nanoscale platform that targets and delivers nanomaterials with biochemicals to plant photosynthetic organelles (chloroplasts) using a guiding peptide recognition motif.
32341352	5	55	theme	function	1092:1099	arg1	changes					1063:1069	more specific tunable changes	1041:1069	more specific tunable changes	1041:1069	Peptide biorecognition provides high delivery efficiency and specificity of QD with chemical cargoes to chloroplasts in plant cells in vivo (74.6 ± 10.8%) and more specific tunable changes of chloroplast redox function than chemicals alone.
32341352	5	55	theme	function	1092:1099	arg1	specificity					943:953	specificity	943:953	specificity	943:953	Peptide biorecognition provides high delivery efficiency and specificity of QD with chemical cargoes to chloroplasts in plant cells in vivo (74.6 ± 10.8%) and more specific tunable changes of chloroplast redox function than chemicals alone.
32341352	5	55	theme	function	1092:1099	arg1	efficiency					928:937	high delivery efficiency	914:937	high delivery efficiency	914:937	Peptide biorecognition provides high delivery efficiency and specificity of QD with chemical cargoes to chloroplasts in plant cells in vivo (74.6 ± 10.8%) and more specific tunable changes of chloroplast redox function than chemicals alone.
32341352	5	55	theme	function	1092:1099	arg1	%					1034:1034	74.6 ± 10.8%	1023:1034	74.6 ± 10.8%	1023:1034	Peptide biorecognition provides high delivery efficiency and specificity of QD with chemical cargoes to chloroplasts in plant cells in vivo (74.6 ± 10.8%) and more specific tunable changes of chloroplast redox function than chemicals alone.
32341352	6	56	from	biology	1270:1276	arg1	agriculture					1348:1358	nano-enabled agriculture	1335:1358	nano-enabled agriculture	1335:1358	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	6	56	from	biology	1270:1276	arg1	range					1224:1228	a broad range	1216:1228	a broad range of nanotechnology applications in plant biology and bioengineering	1216:1295	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	6	56	from	biology	1270:1276	arg1	interactions					1317:1328	nanoparticle-plant interactions	1298:1328	nanoparticle-plant interactions	1298:1328	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	5	57	with	QD	958:959	arg1	cargoes					975:981	chemical cargoes	966:981	chemical cargoes	966:981	Peptide biorecognition provides high delivery efficiency and specificity of QD with chemical cargoes to chloroplasts in plant cells in vivo (74.6 ± 10.8%) and more specific tunable changes of chloroplast redox function than chemicals alone.
32341352	5	58	theme	QD	958:959	arg1	changes					1063:1069	more specific tunable changes	1041:1069	more specific tunable changes	1041:1069	Peptide biorecognition provides high delivery efficiency and specificity of QD with chemical cargoes to chloroplasts in plant cells in vivo (74.6 ± 10.8%) and more specific tunable changes of chloroplast redox function than chemicals alone.
32341352	5	58	theme	QD	958:959	arg1	specificity					943:953	specificity	943:953	specificity	943:953	Peptide biorecognition provides high delivery efficiency and specificity of QD with chemical cargoes to chloroplasts in plant cells in vivo (74.6 ± 10.8%) and more specific tunable changes of chloroplast redox function than chemicals alone.
32341352	5	58	theme	QD	958:959	arg1	efficiency					928:937	high delivery efficiency	914:937	high delivery efficiency	914:937	Peptide biorecognition provides high delivery efficiency and specificity of QD with chemical cargoes to chloroplasts in plant cells in vivo (74.6 ± 10.8%) and more specific tunable changes of chloroplast redox function than chemicals alone.
32341352	5	58	theme	QD	958:959	arg1	%					1034:1034	74.6 ± 10.8%	1023:1034	74.6 ± 10.8%	1023:1034	Peptide biorecognition provides high delivery efficiency and specificity of QD with chemical cargoes to chloroplasts in plant cells in vivo (74.6 ± 10.8%) and more specific tunable changes of chloroplast redox function than chemicals alone.
32341352	5	59	theme	specific	1046:1053	arg1	changes					1063:1069	more specific tunable changes	1041:1069	more specific tunable changes	1041:1069	Peptide biorecognition provides high delivery efficiency and specificity of QD with chemical cargoes to chloroplasts in plant cells in vivo (74.6 ± 10.8%) and more specific tunable changes of chloroplast redox function than chemicals alone.
32341352	1	60	theme	nanomaterial	125:136	arg1	delivery					138:145	nanomaterial delivery	125:145	nanomaterial delivery in plants	125:155	Current approaches for nanomaterial delivery in plants are unable to target specific subcellular compartments with high precision, limiting our ability to engineer plant function.
32341352	4	61	theme	designed	803:810	arg1	peptide					834:840	a rationally designed and conserved guiding peptide	790:840	a rationally designed and conserved guiding peptide	790:840	QD functionalization with β-cyclodextrin molecular baskets enables loading and delivery of diverse chemicals, and nanoparticle coating with a rationally designed and conserved guiding peptide targets their delivery to chloroplasts.
32341352	4	62	theme	guiding	826:832	arg1	peptide					834:840	a rationally designed and conserved guiding peptide	790:840	a rationally designed and conserved guiding peptide	790:840	QD functionalization with β-cyclodextrin molecular baskets enables loading and delivery of diverse chemicals, and nanoparticle coating with a rationally designed and conserved guiding peptide targets their delivery to chloroplasts.
32341352	6	63	theme	nanoparticle-plant	1298:1315	arg1	interactions					1317:1328	nanoparticle-plant interactions	1298:1328	nanoparticle-plant interactions	1298:1328	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	5	64	theme	tunable	1055:1061	arg1	changes					1063:1069	more specific tunable changes	1041:1069	more specific tunable changes	1041:1069	Peptide biorecognition provides high delivery efficiency and specificity of QD with chemical cargoes to chloroplasts in plant cells in vivo (74.6 ± 10.8%) and more specific tunable changes of chloroplast redox function than chemicals alone.
32341352	4	65	theme	nanoparticle	764:775	arg1	coating					777:783	nanoparticle coating	764:783	nanoparticle coating with a rationally designed and conserved guiding peptide	764:840	QD functionalization with β-cyclodextrin molecular baskets enables loading and delivery of diverse chemicals, and nanoparticle coating with a rationally designed and conserved guiding peptide targets their delivery to chloroplasts.
32341352	3	66	theme	fluorescence	486:497	arg1	emission					499:506	Quantum dot (QD) fluorescence emission	469:506	Quantum dot (QD) fluorescence emission in a low background window	469:533	Quantum dot (QD) fluorescence emission in a low background window allows confocal microscopy imaging and quantitative detection by elemental analysis in plant cells and organelles.
32341352	4	67	with	functionalization	653:669	arg1	baskets					701:707	β-cyclodextrin molecular baskets	676:707	β-cyclodextrin molecular baskets	676:707	QD functionalization with β-cyclodextrin molecular baskets enables loading and delivery of diverse chemicals, and nanoparticle coating with a rationally designed and conserved guiding peptide targets their delivery to chloroplasts.
32341352	3	68	theme	QD	482:483	arg1	emission					499:506	Quantum dot (QD) fluorescence emission	469:506	Quantum dot (QD) fluorescence emission in a low background window	469:533	Quantum dot (QD) fluorescence emission in a low background window allows confocal microscopy imaging and quantitative detection by elemental analysis in plant cells and organelles.
32341352	2	69	theme	photosynthetic	385:398	arg1	chloroplasts					412:423	plant photosynthetic organelles (chloroplasts)	379:424	plant photosynthetic organelles (chloroplasts) using a guiding peptide recognition motif	379:466	We demonstrate a nanoscale platform that targets and delivers nanomaterials with biochemicals to plant photosynthetic organelles (chloroplasts) using a guiding peptide recognition motif.
32341352	6	70	theme	nanomaterials	1144:1156	arg1	delivery					1132:1139	Targeted delivery	1123:1139	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs	1123:1210	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	4	71	theme	QD	650:651	arg1	functionalization					653:669	QD functionalization	650:669	QD functionalization with β-cyclodextrin molecular baskets	650:707	QD functionalization with β-cyclodextrin molecular baskets enables loading and delivery of diverse chemicals, and nanoparticle coating with a rationally designed and conserved guiding peptide targets their delivery to chloroplasts.
32341352	4	72	with	coating	777:783	arg1	peptide					834:840	a rationally designed and conserved guiding peptide	790:840	a rationally designed and conserved guiding peptide	790:840	QD functionalization with β-cyclodextrin molecular baskets enables loading and delivery of diverse chemicals, and nanoparticle coating with a rationally designed and conserved guiding peptide targets their delivery to chloroplasts.
32341352	5	73	theme	biorecognition	890:903	arg1	Peptide					882:888	Peptide biorecognition	882:903	Peptide biorecognition	882:903	Peptide biorecognition provides high delivery efficiency and specificity of QD with chemical cargoes to chloroplasts in plant cells in vivo (74.6 ± 10.8%) and more specific tunable changes of chloroplast redox function than chemicals alone.
32341352	2	74	theme	plant	379:383	arg1	chloroplasts					412:423	plant photosynthetic organelles (chloroplasts)	379:424	plant photosynthetic organelles (chloroplasts) using a guiding peptide recognition motif	379:466	We demonstrate a nanoscale platform that targets and delivers nanomaterials with biochemicals to plant photosynthetic organelles (chloroplasts) using a guiding peptide recognition motif.
32341352	6	75	from	bioengineering	1282:1295	arg1	agriculture					1348:1358	nano-enabled agriculture	1335:1358	nano-enabled agriculture	1335:1358	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	6	75	from	bioengineering	1282:1295	arg1	range					1224:1228	a broad range	1216:1228	a broad range of nanotechnology applications in plant biology and bioengineering	1216:1295	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
32341352	6	75	from	bioengineering	1282:1295	arg1	interactions					1317:1328	nanoparticle-plant interactions	1298:1328	nanoparticle-plant interactions	1298:1328	Targeted delivery of nanomaterials with chemical cargoes guided by biorecognition motifs has a broad range of nanotechnology applications in plant biology and bioengineering, nanoparticle-plant interactions, and nano-enabled agriculture.
34641464	6	0	theme	maltose	905:911	arg1	fructose					917:924	fructose	917:924	fructose	917:924	Using a combination of maltose and fructose, the progressive motility of frozen/thawed semen and the fertility rates of eggs were significantly higher ((p < 0.05) 40.2% and 68.5%, respectively) than when using a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively).
34641464	6	0	theme	maltose	905:911	arg1	combination					890:900	a combination	888:900	a combination of maltose	888:911	Using a combination of maltose and fructose, the progressive motility of frozen/thawed semen and the fertility rates of eggs were significantly higher ((p < 0.05) 40.2% and 68.5%, respectively) than when using a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively).
34641464	3	1	theme	non-reducing	502:513	arg1	molecules					528:536	non-reducing disaccharide molecules	502:536	non-reducing disaccharide molecules (trehalose-Treh20 medium)	502:562	The degree of penetration of reducing saccharide molecules (maltose-Mal20 medium) and non-reducing disaccharide molecules (trehalose-Treh20 medium) from the cryoprotective medium into the cytosol of rooster spermatozoa was studied.
34641464	3	1	theme	non-reducing	502:513	arg1	medium					556:561	trehalose-Treh20 medium	539:561	trehalose-Treh20 medium	539:561	The degree of penetration of reducing saccharide molecules (maltose-Mal20 medium) and non-reducing disaccharide molecules (trehalose-Treh20 medium) from the cryoprotective medium into the cytosol of rooster spermatozoa was studied.
34641464	0	2	theme	Rooster	72:78	arg1	Semen					80:84	Rooster Semen	72:84	Rooster Semen	72:84	Role of Mono- and Disaccharide Combination in Cryoprotective Medium for Rooster Semen to Ensure Cryoresistance of Spermatozoa.
34641464	8	3	theme	genital	1389:1395	arg1	tract					1397:1401	the genital tract	1385:1401	the genital tract of hens	1385:1409	Maltose positively affected the preservation of frozen/thawed sperm in the genital tract of hens.
34641464	8	4	theme	frozen/thawed	1362:1374	arg1	sperm					1376:1380	frozen/thawed sperm	1362:1380	frozen/thawed sperm	1362:1380	Maltose positively affected the preservation of frozen/thawed sperm in the genital tract of hens.
34641464	3	5	theme	disaccharide	515:526	arg1	molecules					528:536	non-reducing disaccharide molecules	502:536	non-reducing disaccharide molecules (trehalose-Treh20 medium)	502:562	The degree of penetration of reducing saccharide molecules (maltose-Mal20 medium) and non-reducing disaccharide molecules (trehalose-Treh20 medium) from the cryoprotective medium into the cytosol of rooster spermatozoa was studied.
34641464	3	5	theme	disaccharide	515:526	arg1	medium					556:561	trehalose-Treh20 medium	539:561	trehalose-Treh20 medium	539:561	The degree of penetration of reducing saccharide molecules (maltose-Mal20 medium) and non-reducing disaccharide molecules (trehalose-Treh20 medium) from the cryoprotective medium into the cytosol of rooster spermatozoa was studied.
34641464	6	6	from	diluent	1152:1158	arg1	combination					1094:1104	a combination	1092:1104	a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively)	1092:1190	Using a combination of maltose and fructose, the progressive motility of frozen/thawed semen and the fertility rates of eggs were significantly higher ((p < 0.05) 40.2% and 68.5%, respectively) than when using a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively).
34641464	6	7	theme	fertility	983:991	arg1	higher					1026:1031	higher	1026:1031	higher	1026:1031	Using a combination of maltose and fructose, the progressive motility of frozen/thawed semen and the fertility rates of eggs were significantly higher ((p < 0.05) 40.2% and 68.5%, respectively) than when using a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively).
34641464	6	7	theme	fertility	983:991	arg1	rates					993:997	the fertility rates	979:997	the fertility rates of eggs	979:1005	Using a combination of maltose and fructose, the progressive motility of frozen/thawed semen and the fertility rates of eggs were significantly higher ((p < 0.05) 40.2% and 68.5%, respectively) than when using a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively).
34641464	10	8	contain	have	1603:1606	arg1	trehalose					1593:1601	trehalose	1593:1601	trehalose	1593:1601	Despite the same molecular weight, maltose and trehalose have different physicochemical and biological properties that determine their function and effectiveness as components of cryoprotective media.
34641464	10	8	contain	have	1603:1606	arg1	maltose					1581:1587	maltose	1581:1587	maltose	1581:1587	Despite the same molecular weight, maltose and trehalose have different physicochemical and biological properties that determine their function and effectiveness as components of cryoprotective media.
34641464	10	8	contain	have	1603:1606	arg2	properties					1649:1658	different physicochemical and biological properties	1608:1658	different physicochemical and biological properties that determine their function and effectiveness as components of cryoprotective media	1608:1744	Despite the same molecular weight, maltose and trehalose have different physicochemical and biological properties that determine their function and effectiveness as components of cryoprotective media.
34641464	0	9	from	Role	0:3	arg1	Medium					61:66	Cryoprotective Medium	46:66	Cryoprotective Medium	46:66	Role of Mono- and Disaccharide Combination in Cryoprotective Medium for Rooster Semen to Ensure Cryoresistance of Spermatozoa.
34641464	6	10	theme	fructose	1123:1130	arg1	combination					1094:1104	a combination	1092:1104	a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively)	1092:1190	Using a combination of maltose and fructose, the progressive motility of frozen/thawed semen and the fertility rates of eggs were significantly higher ((p < 0.05) 40.2% and 68.5%, respectively) than when using a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively).
34641464	9	11	theme	seventh	1419:1425	arg1	day					1427:1429	the seventh day	1415:1429	the seventh day from the last insemination when using Mal20	1415:1473	On the seventh day from the last insemination when using Mal20, the fertilization of eggs was 42.6% and only 27.3% when using Treh20.
34641464	6	12	theme	semen	969:973	arg1	higher					1026:1031	higher	1026:1031	higher	1026:1031	Using a combination of maltose and fructose, the progressive motility of frozen/thawed semen and the fertility rates of eggs were significantly higher ((p < 0.05) 40.2% and 68.5%, respectively) than when using a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively).
34641464	6	12	theme	semen	969:973	arg1	rates					993:997	the fertility rates	979:997	the fertility rates of eggs	979:1005	Using a combination of maltose and fructose, the progressive motility of frozen/thawed semen and the fertility rates of eggs were significantly higher ((p < 0.05) 40.2% and 68.5%, respectively) than when using a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively).
34641464	6	12	theme	semen	969:973	arg1	motility					943:950	the progressive motility	927:950	the progressive motility of frozen/thawed semen	927:973	Using a combination of maltose and fructose, the progressive motility of frozen/thawed semen and the fertility rates of eggs were significantly higher ((p < 0.05) 40.2% and 68.5%, respectively) than when using a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively).
34641464	6	13	theme	cryoprotective	1137:1150	arg1	diluent					1152:1158	a cryoprotective diluent	1135:1158	a cryoprotective diluent (33.4% and 62.4%, respectively)	1135:1190	Using a combination of maltose and fructose, the progressive motility of frozen/thawed semen and the fertility rates of eggs were significantly higher ((p < 0.05) 40.2% and 68.5%, respectively) than when using a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively).
34641464	6	14	dep	higher	1026:1031	arg1	%					1049:1049	40.2%	1045:1049	40.2%	1045:1049	Using a combination of maltose and fructose, the progressive motility of frozen/thawed semen and the fertility rates of eggs were significantly higher ((p < 0.05) 40.2% and 68.5%, respectively) than when using a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively).
34641464	6	14	dep	higher	1026:1031	arg1	%					1059:1059	68.5%	1055:1059	68.5%	1055:1059	Using a combination of maltose and fructose, the progressive motility of frozen/thawed semen and the fertility rates of eggs were significantly higher ((p < 0.05) 40.2% and 68.5%, respectively) than when using a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively).
34641464	6	14	dep	higher	1026:1031	arg1	<					1037:1037	p < 0.05	1035:1042	p < 0.05	1035:1042	Using a combination of maltose and fructose, the progressive motility of frozen/thawed semen and the fertility rates of eggs were significantly higher ((p < 0.05) 40.2% and 68.5%, respectively) than when using a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively).
34641464	6	15	from	trehalose	1109:1117	arg1	diluent					1152:1158	a cryoprotective diluent	1135:1158	a cryoprotective diluent (33.4% and 62.4%, respectively)	1135:1190	Using a combination of maltose and fructose, the progressive motility of frozen/thawed semen and the fertility rates of eggs were significantly higher ((p < 0.05) 40.2% and 68.5%, respectively) than when using a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively).
34641464	1	16	theme	reproductive	267:278	arg1	cells					280:284	the reproductive cells	263:284	the reproductive cells of male birds	263:298	The combination of saccharides in the composition of a cryopreservation medium may represent a promising method for the preservation of the reproductive cells of male birds.
34641464	8	17	from	preservation	1346:1357	arg1	tract					1397:1401	the genital tract	1385:1401	the genital tract of hens	1385:1409	Maltose positively affected the preservation of frozen/thawed sperm in the genital tract of hens.
34641464	7	18	theme	integrity	1220:1228	arg1	rate					1202:1205	A higher rate	1193:1205	A higher rate of chromatin integrity at the level of 92.4%	1193:1250	A higher rate of chromatin integrity at the level of 92.4% was obtained when using Treh20 versus 74.5% Mal20 (p < 0.05).
34641464	6	19	theme	trehalose	1109:1117	arg1	combination					1094:1104	a combination	1092:1104	a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively)	1092:1190	Using a combination of maltose and fructose, the progressive motility of frozen/thawed semen and the fertility rates of eggs were significantly higher ((p < 0.05) 40.2% and 68.5%, respectively) than when using a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively).
34641464	2	20	theme	di-saccharides	385:398	arg1	composition					360:370	a combined composition	349:370	a combined composition of mono- and di-saccharides	349:398	In the current study, cryoprotective media with a combined composition of mono- and di-saccharides were developed.
34641464	6	21	from	fructose	1123:1130	arg1	diluent					1152:1158	a cryoprotective diluent	1135:1158	a cryoprotective diluent (33.4% and 62.4%, respectively)	1135:1190	Using a combination of maltose and fructose, the progressive motility of frozen/thawed semen and the fertility rates of eggs were significantly higher ((p < 0.05) 40.2% and 68.5%, respectively) than when using a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively).
34641464	8	22	theme	hens	1406:1409	arg1	tract					1397:1401	the genital tract	1385:1401	the genital tract of hens	1385:1409	Maltose positively affected the preservation of frozen/thawed sperm in the genital tract of hens.
34641464	1	23	from	combination	131:141	arg1	composition					165:175	the composition	161:175	the composition of a cryopreservation medium	161:204	The combination of saccharides in the composition of a cryopreservation medium may represent a promising method for the preservation of the reproductive cells of male birds.
34641464	2	24	theme	current	308:314	arg1	study					316:320	the current study	304:320	the current study	304:320	In the current study, cryoprotective media with a combined composition of mono- and di-saccharides were developed.
34641464	2	25	theme	mono-	375:379	arg1	composition					360:370	a combined composition	349:370	a combined composition of mono- and di-saccharides	349:398	In the current study, cryoprotective media with a combined composition of mono- and di-saccharides were developed.
34641464	1	26	theme	cells	280:284	arg1	preservation					247:258	the preservation	243:258	the preservation of the reproductive cells of male birds	243:298	The combination of saccharides in the composition of a cryopreservation medium may represent a promising method for the preservation of the reproductive cells of male birds.
34641464	10	27	theme	media	1740:1744	arg1	components					1711:1720	components	1711:1720	components of cryoprotective media	1711:1744	Despite the same molecular weight, maltose and trehalose have different physicochemical and biological properties that determine their function and effectiveness as components of cryoprotective media.
34641464	5	28	theme	molecules	856:864	arg1	number					836:841	the number	832:841	the number of trehalose molecules	832:864	The number of maltose molecules penetrating from the outside into the cytosol of the spermatozoon was 1.06 × 104, and the number of trehalose molecules was 3.98 × 104.
34641464	5	28	theme	molecules	856:864	arg1	×					875:875	3.98 × 104	870:879	3.98 × 104	870:879	The number of maltose molecules penetrating from the outside into the cytosol of the spermatozoon was 1.06 × 104, and the number of trehalose molecules was 3.98 × 104.
34641464	10	29	theme	physicochemical	1618:1632	arg1	properties					1649:1658	different physicochemical and biological properties	1608:1658	different physicochemical and biological properties that determine their function and effectiveness as components of cryoprotective media	1608:1744	Despite the same molecular weight, maltose and trehalose have different physicochemical and biological properties that determine their function and effectiveness as components of cryoprotective media.
34641464	1	30	theme	cryopreservation	182:197	arg1	medium					199:204	a cryopreservation medium	180:204	a cryopreservation medium	180:204	The combination of saccharides in the composition of a cryopreservation medium may represent a promising method for the preservation of the reproductive cells of male birds.
34641464	6	31	theme	frozen/thawed	955:967	arg1	semen					969:973	frozen/thawed semen	955:973	frozen/thawed semen	955:973	Using a combination of maltose and fructose, the progressive motility of frozen/thawed semen and the fertility rates of eggs were significantly higher ((p < 0.05) 40.2% and 68.5%, respectively) than when using a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively).
34641464	9	32	theme	last	1440:1443	arg1	insemination					1445:1456	the last insemination	1436:1456	the last insemination when using Mal20	1436:1473	On the seventh day from the last insemination when using Mal20, the fertilization of eggs was 42.6% and only 27.3% when using Treh20.
34641464	1	33	theme	medium	199:204	arg1	composition					165:175	the composition	161:175	the composition of a cryopreservation medium	161:204	The combination of saccharides in the composition of a cryopreservation medium may represent a promising method for the preservation of the reproductive cells of male birds.
34641464	0	34	theme	Mono-	8:12	arg1	Role					0:3	Role	0:3	Role of Mono- and Disaccharide Combination in Cryoprotective Medium for Rooster Semen	0:84	Role of Mono- and Disaccharide Combination in Cryoprotective Medium for Rooster Semen to Ensure Cryoresistance of Spermatozoa.
34641464	1	35	theme	male	289:292	arg1	birds					294:298	male birds	289:298	male birds	289:298	The combination of saccharides in the composition of a cryopreservation medium may represent a promising method for the preservation of the reproductive cells of male birds.
34641464	0	36	theme	Spermatozoa	114:124	arg1	Cryoresistance					96:109	Cryoresistance	96:109	Cryoresistance of Spermatozoa	96:124	Role of Mono- and Disaccharide Combination in Cryoprotective Medium for Rooster Semen to Ensure Cryoresistance of Spermatozoa.
34641464	10	37	theme	cryoprotective	1725:1738	arg1	media					1740:1744	cryoprotective media	1725:1744	cryoprotective media	1725:1744	Despite the same molecular weight, maltose and trehalose have different physicochemical and biological properties that determine their function and effectiveness as components of cryoprotective media.
34641464	3	38	theme	cryoprotective	573:586	arg1	medium					588:593	the cryoprotective medium	569:593	the cryoprotective medium	569:593	The degree of penetration of reducing saccharide molecules (maltose-Mal20 medium) and non-reducing disaccharide molecules (trehalose-Treh20 medium) from the cryoprotective medium into the cytosol of rooster spermatozoa was studied.
34641464	6	39	from	combination	1094:1104	arg1	diluent					1152:1158	a cryoprotective diluent	1135:1158	a cryoprotective diluent (33.4% and 62.4%, respectively)	1135:1190	Using a combination of maltose and fructose, the progressive motility of frozen/thawed semen and the fertility rates of eggs were significantly higher ((p < 0.05) 40.2% and 68.5%, respectively) than when using a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively).
34641464	4	40	theme	LCM	648:650	arg1	media					660:664	LCM control media	648:664	LCM control media without disaccharides	648:686	LCM control media without disaccharides were used as the control.
34641464	4	40	theme	LCM	648:650	arg1	control					705:711	the control	701:711	the control	701:711	LCM control media without disaccharides were used as the control.
34641464	0	41	theme	Combination	31:41	arg1	Role					0:3	Role	0:3	Role of Mono- and Disaccharide Combination in Cryoprotective Medium for Rooster Semen	0:84	Role of Mono- and Disaccharide Combination in Cryoprotective Medium for Rooster Semen to Ensure Cryoresistance of Spermatozoa.
34641464	1	42	theme	birds	294:298	arg1	cells					280:284	the reproductive cells	263:284	the reproductive cells of male birds	263:298	The combination of saccharides in the composition of a cryopreservation medium may represent a promising method for the preservation of the reproductive cells of male birds.
34641464	5	43	theme	maltose	728:734	arg1	molecules					736:744	maltose molecules	728:744	maltose molecules penetrating from the outside into the cytosol of the spermatozoon	728:810	The number of maltose molecules penetrating from the outside into the cytosol of the spermatozoon was 1.06 × 104, and the number of trehalose molecules was 3.98 × 104.
34641464	9	44	from	%	1525:1525	arg1	day					1427:1429	the seventh day	1415:1429	the seventh day from the last insemination when using Mal20	1415:1473	On the seventh day from the last insemination when using Mal20, the fertilization of eggs was 42.6% and only 27.3% when using Treh20.
34641464	0	45	theme	Disaccharide	18:29	arg1	Combination					31:41	Disaccharide Combination	18:41	Disaccharide Combination	18:41	Role of Mono- and Disaccharide Combination in Cryoprotective Medium for Rooster Semen to Ensure Cryoresistance of Spermatozoa.
34641464	6	46	theme	progressive	931:941	arg1	higher					1026:1031	higher	1026:1031	higher	1026:1031	Using a combination of maltose and fructose, the progressive motility of frozen/thawed semen and the fertility rates of eggs were significantly higher ((p < 0.05) 40.2% and 68.5%, respectively) than when using a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively).
34641464	6	46	theme	progressive	931:941	arg1	motility					943:950	the progressive motility	927:950	the progressive motility of frozen/thawed semen	927:973	Using a combination of maltose and fructose, the progressive motility of frozen/thawed semen and the fertility rates of eggs were significantly higher ((p < 0.05) 40.2% and 68.5%, respectively) than when using a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively).
34641464	5	47	theme	molecules	736:744	arg1	×					821:821	1.06 × 104	816:825	1.06 × 104	816:825	The number of maltose molecules penetrating from the outside into the cytosol of the spermatozoon was 1.06 × 104, and the number of trehalose molecules was 3.98 × 104.
34641464	5	47	theme	molecules	736:744	arg1	number					718:723	The number	714:723	The number of maltose molecules penetrating from the outside into the cytosol of the spermatozoon	714:810	The number of maltose molecules penetrating from the outside into the cytosol of the spermatozoon was 1.06 × 104, and the number of trehalose molecules was 3.98 × 104.
34641464	2	48	with	media	338:342	arg1	composition					360:370	a combined composition	349:370	a combined composition of mono- and di-saccharides	349:398	In the current study, cryoprotective media with a combined composition of mono- and di-saccharides were developed.
34641464	3	49	theme	rooster	615:621	arg1	spermatozoa					623:633	rooster spermatozoa	615:633	rooster spermatozoa	615:633	The degree of penetration of reducing saccharide molecules (maltose-Mal20 medium) and non-reducing disaccharide molecules (trehalose-Treh20 medium) from the cryoprotective medium into the cytosol of rooster spermatozoa was studied.
34641464	4	50	used	used	693:696	arg2	control					705:711	the control	701:711	the control	701:711	LCM control media without disaccharides were used as the control.
34641464	4	50	used	used	693:696	arg2	media					660:664	LCM control media	648:664	LCM control media without disaccharides	648:686	LCM control media without disaccharides were used as the control.
34641464	3	51	theme	saccharide	454:463	arg1	molecules					465:473	saccharide molecules	454:473	saccharide molecules (maltose-Mal20 medium)	454:496	The degree of penetration of reducing saccharide molecules (maltose-Mal20 medium) and non-reducing disaccharide molecules (trehalose-Treh20 medium) from the cryoprotective medium into the cytosol of rooster spermatozoa was studied.
34641464	3	51	theme	saccharide	454:463	arg1	medium					490:495	maltose-Mal20 medium	476:495	maltose-Mal20 medium	476:495	The degree of penetration of reducing saccharide molecules (maltose-Mal20 medium) and non-reducing disaccharide molecules (trehalose-Treh20 medium) from the cryoprotective medium into the cytosol of rooster spermatozoa was studied.
34641464	0	52	theme	Cryoprotective	46:59	arg1	Medium					61:66	Cryoprotective Medium	46:66	Cryoprotective Medium	46:66	Role of Mono- and Disaccharide Combination in Cryoprotective Medium for Rooster Semen to Ensure Cryoresistance of Spermatozoa.
34641464	9	53	from	%	1510:1510	arg1	day					1427:1429	the seventh day	1415:1429	the seventh day from the last insemination when using Mal20	1415:1473	On the seventh day from the last insemination when using Mal20, the fertilization of eggs was 42.6% and only 27.3% when using Treh20.
34641464	3	54	theme	penetration	430:440	arg1	degree					420:425	The degree	416:425	The degree of penetration of reducing saccharide molecules (maltose-Mal20 medium) and non-reducing disaccharide molecules (trehalose-Treh20 medium) from the cryoprotective medium into the cytosol of rooster spermatozoa	416:633	The degree of penetration of reducing saccharide molecules (maltose-Mal20 medium) and non-reducing disaccharide molecules (trehalose-Treh20 medium) from the cryoprotective medium into the cytosol of rooster spermatozoa was studied.
34641464	8	55	theme	sperm	1376:1380	arg1	preservation					1346:1357	the preservation	1342:1357	the preservation of frozen/thawed sperm in the genital tract of hens	1342:1409	Maltose positively affected the preservation of frozen/thawed sperm in the genital tract of hens.
34641464	10	56	theme	molecular	1563:1571	arg1	weight					1573:1578	the same molecular weight	1554:1578	the same molecular weight	1554:1578	Despite the same molecular weight, maltose and trehalose have different physicochemical and biological properties that determine their function and effectiveness as components of cryoprotective media.
34641464	10	57	theme	biological	1638:1647	arg1	properties					1649:1658	different physicochemical and biological properties	1608:1658	different physicochemical and biological properties that determine their function and effectiveness as components of cryoprotective media	1608:1744	Despite the same molecular weight, maltose and trehalose have different physicochemical and biological properties that determine their function and effectiveness as components of cryoprotective media.
34641464	2	58	theme	cryoprotective	323:336	arg1	media					338:342	cryoprotective media	323:342	cryoprotective media with a combined composition of mono- and di-saccharides	323:398	In the current study, cryoprotective media with a combined composition of mono- and di-saccharides were developed.
34641464	7	59	theme	%	1250:1250	arg1	level					1237:1241	the level	1233:1241	the level of 92.4%	1233:1250	A higher rate of chromatin integrity at the level of 92.4% was obtained when using Treh20 versus 74.5% Mal20 (p < 0.05).
34641464	7	60	theme	p	1303:1303	arg1	<					1305:1305	p < 0.05	1303:1310	p < 0.05	1303:1310	A higher rate of chromatin integrity at the level of 92.4% was obtained when using Treh20 versus 74.5% Mal20 (p < 0.05).
34641464	7	60	theme	p	1303:1303	arg1	%					1294:1294	74.5% Mal20	1290:1300	74.5% Mal20 (p < 0.05)	1290:1311	A higher rate of chromatin integrity at the level of 92.4% was obtained when using Treh20 versus 74.5% Mal20 (p < 0.05).
34641464	6	61	dep	diluent	1152:1158	arg1	%					1165:1165	33.4%	1161:1165	33.4%	1161:1165	Using a combination of maltose and fructose, the progressive motility of frozen/thawed semen and the fertility rates of eggs were significantly higher ((p < 0.05) 40.2% and 68.5%, respectively) than when using a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively).
34641464	6	61	dep	diluent	1152:1158	arg1	%					1175:1175	62.4%	1171:1175	62.4%	1171:1175	Using a combination of maltose and fructose, the progressive motility of frozen/thawed semen and the fertility rates of eggs were significantly higher ((p < 0.05) 40.2% and 68.5%, respectively) than when using a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively).
34641464	9	62	from	insemination	1445:1456	arg1	day					1427:1429	the seventh day	1415:1429	the seventh day from the last insemination when using Mal20	1415:1473	On the seventh day from the last insemination when using Mal20, the fertilization of eggs was 42.6% and only 27.3% when using Treh20.
34641464	1	63	theme	promising	222:230	arg1	method					232:237	a promising method	220:237	a promising method for the preservation of the reproductive cells of male birds	220:298	The combination of saccharides in the composition of a cryopreservation medium may represent a promising method for the preservation of the reproductive cells of male birds.
34641464	7	64	theme	higher	1195:1200	arg1	rate					1202:1205	A higher rate	1193:1205	A higher rate of chromatin integrity at the level of 92.4%	1193:1250	A higher rate of chromatin integrity at the level of 92.4% was obtained when using Treh20 versus 74.5% Mal20 (p < 0.05).
34641464	6	65	theme	p	1035:1035	arg1	%					1049:1049	40.2%	1045:1049	40.2%	1045:1049	Using a combination of maltose and fructose, the progressive motility of frozen/thawed semen and the fertility rates of eggs were significantly higher ((p < 0.05) 40.2% and 68.5%, respectively) than when using a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively).
34641464	6	65	theme	p	1035:1035	arg1	<					1037:1037	p < 0.05	1035:1042	p < 0.05	1035:1042	Using a combination of maltose and fructose, the progressive motility of frozen/thawed semen and the fertility rates of eggs were significantly higher ((p < 0.05) 40.2% and 68.5%, respectively) than when using a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively).
34641464	1	66	theme	saccharides	146:156	arg1	combination					131:141	The combination	127:141	The combination of saccharides in the composition of a cryopreservation medium	127:204	The combination of saccharides in the composition of a cryopreservation medium may represent a promising method for the preservation of the reproductive cells of male birds.
34641464	10	67	theme	different	1608:1616	arg1	properties					1649:1658	different physicochemical and biological properties	1608:1658	different physicochemical and biological properties that determine their function and effectiveness as components of cryoprotective media	1608:1744	Despite the same molecular weight, maltose and trehalose have different physicochemical and biological properties that determine their function and effectiveness as components of cryoprotective media.
34641464	5	68	theme	trehalose	846:854	arg1	molecules					856:864	trehalose molecules	846:864	trehalose molecules	846:864	The number of maltose molecules penetrating from the outside into the cytosol of the spermatozoon was 1.06 × 104, and the number of trehalose molecules was 3.98 × 104.
34641464	3	69	theme	spermatozoa	623:633	arg1	cytosol					604:610	the cytosol	600:610	the cytosol of rooster spermatozoa	600:633	The degree of penetration of reducing saccharide molecules (maltose-Mal20 medium) and non-reducing disaccharide molecules (trehalose-Treh20 medium) from the cryoprotective medium into the cytosol of rooster spermatozoa was studied.
34641464	4	70	theme	control	652:658	arg1	media					660:664	LCM control media	648:664	LCM control media without disaccharides	648:686	LCM control media without disaccharides were used as the control.
34641464	4	70	theme	control	652:658	arg1	control					705:711	the control	701:711	the control	701:711	LCM control media without disaccharides were used as the control.
34641464	10	71	theme	same	1558:1561	arg1	weight					1573:1578	the same molecular weight	1554:1578	the same molecular weight	1554:1578	Despite the same molecular weight, maltose and trehalose have different physicochemical and biological properties that determine their function and effectiveness as components of cryoprotective media.
34641464	7	72	theme	chromatin	1210:1218	arg1	integrity					1220:1228	chromatin integrity	1210:1228	chromatin integrity	1210:1228	A higher rate of chromatin integrity at the level of 92.4% was obtained when using Treh20 versus 74.5% Mal20 (p < 0.05).
34641464	6	73	theme	eggs	1002:1005	arg1	higher					1026:1031	higher	1026:1031	higher	1026:1031	Using a combination of maltose and fructose, the progressive motility of frozen/thawed semen and the fertility rates of eggs were significantly higher ((p < 0.05) 40.2% and 68.5%, respectively) than when using a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively).
34641464	6	73	theme	eggs	1002:1005	arg1	rates					993:997	the fertility rates	979:997	the fertility rates of eggs	979:1005	Using a combination of maltose and fructose, the progressive motility of frozen/thawed semen and the fertility rates of eggs were significantly higher ((p < 0.05) 40.2% and 68.5%, respectively) than when using a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively).
34641464	6	73	theme	eggs	1002:1005	arg1	motility					943:950	the progressive motility	927:950	the progressive motility of frozen/thawed semen	927:973	Using a combination of maltose and fructose, the progressive motility of frozen/thawed semen and the fertility rates of eggs were significantly higher ((p < 0.05) 40.2% and 68.5%, respectively) than when using a combination of trehalose and fructose in a cryoprotective diluent (33.4% and 62.4%, respectively).
34641464	5	74	theme	spermatozoon	799:810	arg1	cytosol					784:790	the cytosol	780:790	the cytosol of the spermatozoon	780:810	The number of maltose molecules penetrating from the outside into the cytosol of the spermatozoon was 1.06 × 104, and the number of trehalose molecules was 3.98 × 104.
34641464	7	75	from	level	1237:1241	arg1	rate					1202:1205	A higher rate	1193:1205	A higher rate of chromatin integrity at the level of 92.4%	1193:1250	A higher rate of chromatin integrity at the level of 92.4% was obtained when using Treh20 versus 74.5% Mal20 (p < 0.05).
34641464	2	76	theme	combined	351:358	arg1	composition					360:370	a combined composition	349:370	a combined composition of mono- and di-saccharides	349:398	In the current study, cryoprotective media with a combined composition of mono- and di-saccharides were developed.
34641464	9	77	theme	eggs	1497:1500	arg1	%					1510:1510	42.6%	1506:1510	42.6%	1506:1510	On the seventh day from the last insemination when using Mal20, the fertilization of eggs was 42.6% and only 27.3% when using Treh20.
34641464	9	77	theme	eggs	1497:1500	arg1	fertilization					1480:1492	the fertilization	1476:1492	the fertilization of eggs	1476:1500	On the seventh day from the last insemination when using Mal20, the fertilization of eggs was 42.6% and only 27.3% when using Treh20.
34851167	12	0	theme	members	2046:2052	arg1	abundance					2028:2036	the abundance	2024:2036	the abundance of some members of the gut microbiome	2024:2074	IMPORTANCE The drug acarbose is used to treat diabetes by preventing the breakdown of starch in the small intestine, resulting in dramatic changes in the abundance of some members of the gut microbiome and its fermentation products.
34851167	17	1	located	found	2790:2794	arg2	genes					2710:2714	genes	2710:2714	genes encoding one type of enzyme known to participate in starch breakdown	2710:2783	In addition, genes encoding one type of enzyme known to participate in starch breakdown were found in all three genomes from responding species but none of the other genomes.
34851167	17	1	located	found	2790:2794	arg1	addition					2700:2707	addition	2700:2707	addition	2700:2707	In addition, genes encoding one type of enzyme known to participate in starch breakdown were found in all three genomes from responding species but none of the other genomes.
34851167	17	1	located	found	2790:2794	arg1	genomes					2809:2815	all three genomes	2799:2815	all three genomes from responding species but none of the other genomes	2799:2869	In addition, genes encoding one type of enzyme known to participate in starch breakdown were found in all three genomes from responding species but none of the other genomes.
34851167	17	2	theme	type	2729:2732	arg1	enzyme					2737:2742	one type of enzyme	2725:2742	one type of enzyme known to participate in starch breakdown	2725:2783	In addition, genes encoding one type of enzyme known to participate in starch breakdown were found in all three genomes from responding species but none of the other genomes.
34851167	4	3	theme	study	587:591	arg1	sites					593:597	three study sites	581:597	three study sites	581:597	In experiments replicated across three study sites, two distantly related species in the bacterial family Muribaculaceae were dramatically more abundant in acarbose-treated mice, distinguishing these responders from other members of the family.
34851167	1	4	theme	starch	251:256	arg1	amount					241:246	the amount	237:246	the amount of starch entering the lower digestive tract	237:291	The drug acarbose is used to treat diabetes and, by inhibiting α-amylase in the small intestine, increases the amount of starch entering the lower digestive tract.
34851167	1	4	theme	starch	251:256	arg1	starch					251:256	starch	251:256	starch	251:256	The drug acarbose is used to treat diabetes and, by inhibiting α-amylase in the small intestine, increases the amount of starch entering the lower digestive tract.
34851167	1	5	used	used	151:154	arg2	acarbose					139:146	The drug acarbose	130:146	The drug acarbose	130:146	The drug acarbose is used to treat diabetes and, by inhibiting α-amylase in the small intestine, increases the amount of starch entering the lower digestive tract.
34851167	4	6	theme	family	647:652	arg1	Muribaculaceae					654:667	the bacterial family Muribaculaceae	633:667	the bacterial family Muribaculaceae	633:667	In experiments replicated across three study sites, two distantly related species in the bacterial family Muribaculaceae were dramatically more abundant in acarbose-treated mice, distinguishing these responders from other members of the family.
34851167	10	7	theme	gene	1579:1582	arg1	content					1584:1590	Differential gene content	1566:1590	Differential gene content in B1A and B1B	1566:1605	Differential gene content in B1A and B1B may be associated with the inconsistent response of this species to acarbose across study sites.
34851167	3	8	theme	acids	517:521	arg1	production					481:490	increased production	471:490	increased production of the short-chain fatty acids propionate and butyrate	471:545	Acarbose also increases longevity in mice, an effect that has been correlated with increased production of the short-chain fatty acids propionate and butyrate.
34851167	5	9	theme	fermentation	872:883	arg1	propionate					856:865	propionate	856:865	propionate	856:865	Bacteria in the family Muribaculaceae are predicted to produce propionate as a fermentation end product and are abundant and diverse in the guts of mice, although few isolates are available.
34851167	5	9	theme	fermentation	872:883	arg1	product					889:895	a fermentation end product	870:895	a fermentation end product	870:895	Bacteria in the family Muribaculaceae are predicted to produce propionate as a fermentation end product and are abundant and diverse in the guts of mice, although few isolates are available.
34851167	7	10	theme	related	1174:1180	arg1	B1B					1196:1198	B1B	1196:1198	B1B	1196:1198	We found two closely related MAGs (B1A and B1B) from one responsive species that both contain a polysaccharide utilization locus with a predicted extracellular α-amylase.
34851167	7	10	theme	related	1174:1180	arg1	B1A					1188:1190	B1A	1188:1190	B1A	1188:1190	We found two closely related MAGs (B1A and B1B) from one responsive species that both contain a polysaccharide utilization locus with a predicted extracellular α-amylase.
34851167	7	10	theme	related	1174:1180	arg1	MAGs					1182:1185	two closely related MAGs	1162:1185	two closely related MAGs (B1A and B1B) from one responsive species that both contain a polysaccharide utilization locus with a predicted extracellular α-amylase	1162:1321	We found two closely related MAGs (B1A and B1B) from one responsive species that both contain a polysaccharide utilization locus with a predicted extracellular α-amylase.
34851167	11	11	theme	gut	1802:1804	arg1	bacteria					1806:1813	gut bacteria	1802:1813	gut bacteria	1802:1813	This work demonstrates the utility of culture-free genomics for inferring the ecological roles of gut bacteria, including their response to pharmaceutical perturbations.
34851167	4	12	from	species	622:628	arg1	Muribaculaceae					654:667	the bacterial family Muribaculaceae	633:667	the bacterial family Muribaculaceae	633:667	In experiments replicated across three study sites, two distantly related species in the bacterial family Muribaculaceae were dramatically more abundant in acarbose-treated mice, distinguishing these responders from other members of the family.
34851167	15	13	from	advantage	2521:2529	arg1	intestine					2544:2552	the large intestine	2534:2552	the large intestine of animals consuming acarbose	2534:2582	We found that genomes of the most responsive Muribaculaceae showed signs of specialization for starch fermentation, presumably providing them a competitive advantage in the large intestine of animals consuming acarbose.
34851167	16	14	theme	family	2689:2694	arg1	members					2673:2679	members	2673:2679	members of this family	2673:2694	Comparisons among genomes enhance existing models for the ecological niches occupied by members of this family.
34851167	12	15	from	products	2097:2104	arg1	abundance					2028:2036	the abundance	2024:2036	the abundance of some members of the gut microbiome	2024:2074	IMPORTANCE The drug acarbose is used to treat diabetes by preventing the breakdown of starch in the small intestine, resulting in dramatic changes in the abundance of some members of the gut microbiome and its fermentation products.
34851167	12	16	theme	small	1974:1978	arg1	intestine					1980:1988	the small intestine	1970:1988	the small intestine	1970:1988	IMPORTANCE The drug acarbose is used to treat diabetes by preventing the breakdown of starch in the small intestine, resulting in dramatic changes in the abundance of some members of the gut microbiome and its fermentation products.
34851167	8	17	theme	other	1447:1451	arg1	species					1464:1470	the only other responsive species	1438:1470	the only other responsive species	1438:1470	These genomes also shared a periplasmic neopullulanase with another, distantly related MAG (B2) representative of the only other responsive species.
34851167	3	18	dep	acids	517:521	arg1	propionate					523:532	propionate	523:532	propionate	523:532	Acarbose also increases longevity in mice, an effect that has been correlated with increased production of the short-chain fatty acids propionate and butyrate.
34851167	3	18	dep	acids	517:521	arg1	acids					517:521	the short-chain fatty acids propionate and butyrate	495:545	the short-chain fatty acids propionate and butyrate	495:545	Acarbose also increases longevity in mice, an effect that has been correlated with increased production of the short-chain fatty acids propionate and butyrate.
34851167	3	18	dep	acids	517:521	arg1	butyrate					538:545	butyrate	538:545	butyrate	538:545	Acarbose also increases longevity in mice, an effect that has been correlated with increased production of the short-chain fatty acids propionate and butyrate.
34851167	8	19	theme	species	1464:1470	arg1	representative					1420:1433	distantly related MAG (B2) representative	1393:1433	distantly related MAG (B2) representative of the only other responsive species	1393:1470	These genomes also shared a periplasmic neopullulanase with another, distantly related MAG (B2) representative of the only other responsive species.
34851167	8	19	theme	species	1464:1470	arg1	neopullulanase					1364:1377	a periplasmic neopullulanase	1350:1377	a periplasmic neopullulanase with another	1350:1390	These genomes also shared a periplasmic neopullulanase with another, distantly related MAG (B2) representative of the only other responsive species.
34851167	14	20	theme	increased	2336:2344	arg1	longevity					2346:2354	increased longevity	2336:2354	increased longevity in mice	2336:2362	Propionate has been associated with gut health and increased longevity in mice.
34851167	12	21	from	breakdown	1947:1955	arg1	intestine					1980:1988	the small intestine	1970:1988	the small intestine	1970:1988	IMPORTANCE The drug acarbose is used to treat diabetes by preventing the breakdown of starch in the small intestine, resulting in dramatic changes in the abundance of some members of the gut microbiome and its fermentation products.
34851167	11	22	theme	culture-free	1742:1753	arg1	genomics					1755:1762	culture-free genomics	1742:1762	culture-free genomics	1742:1762	This work demonstrates the utility of culture-free genomics for inferring the ecological roles of gut bacteria, including their response to pharmaceutical perturbations.
34851167	7	23	theme	responsive	1210:1219	arg1	species					1221:1227	one responsive species	1206:1227	one responsive species that both contain a polysaccharide utilization locus with a predicted extracellular α-amylase	1206:1321	We found two closely related MAGs (B1A and B1B) from one responsive species that both contain a polysaccharide utilization locus with a predicted extracellular α-amylase.
34851167	17	24	theme	responding	2822:2831	arg1	species					2833:2839	responding species	2822:2839	responding species	2822:2839	In addition, genes encoding one type of enzyme known to participate in starch breakdown were found in all three genomes from responding species but none of the other genomes.
34851167	6	25	from	metagenomes	1014:1024	arg1	genomes					1001:1007	genomes	1001:1007	genomes from metagenomes (MAGs) for nine populations of Muribaculaceae	1001:1070	We reconstructed genomes from metagenomes (MAGs) for nine populations of Muribaculaceae to examine factors that distinguish species that respond positively to acarbose.
34851167	12	26	theme	microbiome	2065:2074	arg1	members					2046:2052	some members	2041:2052	some members of the gut microbiome	2041:2074	IMPORTANCE The drug acarbose is used to treat diabetes by preventing the breakdown of starch in the small intestine, resulting in dramatic changes in the abundance of some members of the gut microbiome and its fermentation products.
34851167	0	27	theme	Response	90:97	arg1	Drivers					66:72	Genetic Drivers	58:72	Genetic Drivers of Differential Response to Acarbose Treatment in Mice	58:127	Muribaculaceae Genomes Assembled from Metagenomes Suggest Genetic Drivers of Differential Response to Acarbose Treatment in Mice.
34851167	7	28	contain	contain	1239:1245	arg1	both					1234:1237	both	1234:1237	both	1234:1237	We found two closely related MAGs (B1A and B1B) from one responsive species that both contain a polysaccharide utilization locus with a predicted extracellular α-amylase.
34851167	7	28	contain	contain	1239:1245	arg2	locus					1276:1280	a polysaccharide utilization locus	1247:1280	a polysaccharide utilization locus	1247:1280	We found two closely related MAGs (B1A and B1B) from one responsive species that both contain a polysaccharide utilization locus with a predicted extracellular α-amylase.
34851167	7	28	contain	contain	1239:1245	arg2	species					1221:1227	one responsive species	1206:1227	one responsive species that both contain a polysaccharide utilization locus with a predicted extracellular α-amylase	1206:1321	We found two closely related MAGs (B1A and B1B) from one responsive species that both contain a polysaccharide utilization locus with a predicted extracellular α-amylase.
34851167	5	29	from	abundant	905:912	arg1	guts					933:936	the guts	929:936	the guts of mice	929:944	Bacteria in the family Muribaculaceae are predicted to produce propionate as a fermentation end product and are abundant and diverse in the guts of mice, although few isolates are available.
34851167	13	30	theme	fermentation	2263:2274	arg1	product					2276:2282	a primary fermentation product	2253:2282	a primary fermentation product	2253:2282	In mice, several of the bacteria that respond most positively are classified in the family Muribaculaceae, members of which produce propionate as a primary fermentation product.
34851167	13	30	theme	fermentation	2263:2274	arg1	propionate					2239:2248	propionate	2239:2248	propionate	2239:2248	In mice, several of the bacteria that respond most positively are classified in the family Muribaculaceae, members of which produce propionate as a primary fermentation product.
34851167	0	31	theme	Acarbose	102:109	arg1	Treatment					111:119	Acarbose Treatment	102:119	Acarbose Treatment in Mice	102:127	Muribaculaceae Genomes Assembled from Metagenomes Suggest Genetic Drivers of Differential Response to Acarbose Treatment in Mice.
34851167	15	32	theme	responsive	2399:2408	arg1	Muribaculaceae					2410:2423	the most responsive Muribaculaceae	2390:2423	the most responsive Muribaculaceae	2390:2423	We found that genomes of the most responsive Muribaculaceae showed signs of specialization for starch fermentation, presumably providing them a competitive advantage in the large intestine of animals consuming acarbose.
34851167	15	33	theme	large	2538:2542	arg1	intestine					2544:2552	the large intestine	2534:2552	the large intestine of animals consuming acarbose	2534:2582	We found that genomes of the most responsive Muribaculaceae showed signs of specialization for starch fermentation, presumably providing them a competitive advantage in the large intestine of animals consuming acarbose.
34851167	9	34	theme	species	1557:1563	arg1	representative					1525:1538	representative	1525:1538	representative	1525:1538	This gene differentiated these three MAGs from MAGs representative of nonresponding species.
34851167	8	35	theme	periplasmic	1352:1362	arg1	representative					1420:1433	distantly related MAG (B2) representative	1393:1433	distantly related MAG (B2) representative of the only other responsive species	1393:1470	These genomes also shared a periplasmic neopullulanase with another, distantly related MAG (B2) representative of the only other responsive species.
34851167	8	35	theme	periplasmic	1352:1362	arg1	neopullulanase					1364:1377	a periplasmic neopullulanase	1350:1377	a periplasmic neopullulanase with another	1350:1390	These genomes also shared a periplasmic neopullulanase with another, distantly related MAG (B2) representative of the only other responsive species.
34851167	10	36	theme	species	1664:1670	arg1	response					1647:1654	the inconsistent response	1630:1654	the inconsistent response of this species to acarbose across study sites	1630:1701	Differential gene content in B1A and B1B may be associated with the inconsistent response of this species to acarbose across study sites.
34851167	0	37	theme	Muribaculaceae	0:13	arg1	Genomes					15:21	Muribaculaceae Genomes	0:21	Muribaculaceae Genomes Assembled from Metagenomes	0:48	Muribaculaceae Genomes Assembled from Metagenomes Suggest Genetic Drivers of Differential Response to Acarbose Treatment in Mice.
34851167	15	38	theme	competitive	2509:2519	arg1	advantage					2521:2529	a competitive advantage	2507:2529	them a competitive advantage in the large intestine of animals consuming acarbose	2502:2582	We found that genomes of the most responsive Muribaculaceae showed signs of specialization for starch fermentation, presumably providing them a competitive advantage in the large intestine of animals consuming acarbose.
34851167	5	39	theme	mice	941:944	arg1	guts					933:936	the guts	929:936	the guts of mice	929:944	Bacteria in the family Muribaculaceae are predicted to produce propionate as a fermentation end product and are abundant and diverse in the guts of mice, although few isolates are available.
34851167	4	40	theme	other	764:768	arg1	members					770:776	other members	764:776	other members of the family	764:790	In experiments replicated across three study sites, two distantly related species in the bacterial family Muribaculaceae were dramatically more abundant in acarbose-treated mice, distinguishing these responders from other members of the family.
34851167	10	41	theme	study	1691:1695	arg1	sites					1697:1701	study sites	1691:1701	study sites	1691:1701	Differential gene content in B1A and B1B may be associated with the inconsistent response of this species to acarbose across study sites.
34851167	1	42	theme	lower	271:275	arg1	tract					287:291	the lower digestive tract	267:291	the lower digestive tract	267:291	The drug acarbose is used to treat diabetes and, by inhibiting α-amylase in the small intestine, increases the amount of starch entering the lower digestive tract.
34851167	12	43	theme	drug	1889:1892	arg1	acarbose					1894:1901	The drug acarbose	1885:1901	IMPORTANCE The drug acarbose	1874:1901	IMPORTANCE The drug acarbose is used to treat diabetes by preventing the breakdown of starch in the small intestine, resulting in dramatic changes in the abundance of some members of the gut microbiome and its fermentation products.
34851167	7	44	theme	extracellular	1299:1311	arg1	α-amylase					1313:1321	a predicted extracellular α-amylase	1287:1321	a predicted extracellular α-amylase	1287:1321	We found two closely related MAGs (B1A and B1B) from one responsive species that both contain a polysaccharide utilization locus with a predicted extracellular α-amylase.
34851167	12	45	used	used	1906:1909	arg2	IMPORTANCE					1874:1883	IMPORTANCE	1874:1883	IMPORTANCE The drug acarbose	1874:1901	IMPORTANCE The drug acarbose is used to treat diabetes by preventing the breakdown of starch in the small intestine, resulting in dramatic changes in the abundance of some members of the gut microbiome and its fermentation products.
34851167	8	46	theme	related	1403:1409	arg1	representative					1420:1433	distantly related MAG (B2) representative	1393:1433	distantly related MAG (B2) representative of the only other responsive species	1393:1470	These genomes also shared a periplasmic neopullulanase with another, distantly related MAG (B2) representative of the only other responsive species.
34851167	8	46	theme	related	1403:1409	arg1	neopullulanase					1364:1377	a periplasmic neopullulanase	1350:1377	a periplasmic neopullulanase with another	1350:1390	These genomes also shared a periplasmic neopullulanase with another, distantly related MAG (B2) representative of the only other responsive species.
34851167	13	47	theme	family	2191:2196	arg1	members					2214:2220	members	2214:2220	members of which produce propionate as a primary fermentation product	2214:2282	In mice, several of the bacteria that respond most positively are classified in the family Muribaculaceae, members of which produce propionate as a primary fermentation product.
34851167	13	47	theme	family	2191:2196	arg1	Muribaculaceae					2198:2211	the family Muribaculaceae	2187:2211	the family Muribaculaceae	2187:2211	In mice, several of the bacteria that respond most positively are classified in the family Muribaculaceae, members of which produce propionate as a primary fermentation product.
34851167	17	48	theme	other	2857:2861	arg1	genomes					2863:2869	the other genomes	2853:2869	the other genomes	2853:2869	In addition, genes encoding one type of enzyme known to participate in starch breakdown were found in all three genomes from responding species but none of the other genomes.
34851167	0	49	theme	Genetic	58:64	arg1	Drivers					66:72	Genetic Drivers	58:72	Genetic Drivers of Differential Response to Acarbose Treatment in Mice	58:127	Muribaculaceae Genomes Assembled from Metagenomes Suggest Genetic Drivers of Differential Response to Acarbose Treatment in Mice.
34851167	10	50	from	content	1584:1590	arg1	B1B					1603:1605	B1B	1603:1605	B1B	1603:1605	Differential gene content in B1A and B1B may be associated with the inconsistent response of this species to acarbose across study sites.
34851167	10	50	from	content	1584:1590	arg1	B1A					1595:1597	B1A	1595:1597	B1A	1595:1597	Differential gene content in B1A and B1B may be associated with the inconsistent response of this species to acarbose across study sites.
34851167	14	51	from	health	2325:2330	arg1	mice					2359:2362	mice	2359:2362	mice	2359:2362	Propionate has been associated with gut health and increased longevity in mice.
34851167	1	52	theme	small	210:214	arg1	intestine					216:224	the small intestine	206:224	the small intestine	206:224	The drug acarbose is used to treat diabetes and, by inhibiting α-amylase in the small intestine, increases the amount of starch entering the lower digestive tract.
34851167	7	53	theme	polysaccharide	1249:1262	arg1	locus					1276:1280	a polysaccharide utilization locus	1247:1280	a polysaccharide utilization locus	1247:1280	We found two closely related MAGs (B1A and B1B) from one responsive species that both contain a polysaccharide utilization locus with a predicted extracellular α-amylase.
34851167	10	54	theme	Differential	1566:1577	arg1	content					1584:1590	Differential gene content	1566:1590	Differential gene content in B1A and B1B	1566:1605	Differential gene content in B1A and B1B may be associated with the inconsistent response of this species to acarbose across study sites.
34851167	2	55	theme	microbiota	344:353	arg1	composition					325:335	the composition	321:335	the composition of the microbiota	321:353	This results in changes to the composition of the microbiota and their fermentation products.
34851167	4	56	from	abundant	692:699	arg1	mice					721:724	acarbose-treated mice	704:724	acarbose-treated mice	704:724	In experiments replicated across three study sites, two distantly related species in the bacterial family Muribaculaceae were dramatically more abundant in acarbose-treated mice, distinguishing these responders from other members of the family.
34851167	4	56	from	abundant	692:699	arg1	experiments					551:561	experiments	551:561	experiments replicated across three study sites	551:597	In experiments replicated across three study sites, two distantly related species in the bacterial family Muribaculaceae were dramatically more abundant in acarbose-treated mice, distinguishing these responders from other members of the family.
34851167	7	57	from	species	1221:1227	arg1	B1B					1196:1198	B1B	1196:1198	B1B	1196:1198	We found two closely related MAGs (B1A and B1B) from one responsive species that both contain a polysaccharide utilization locus with a predicted extracellular α-amylase.
34851167	7	57	from	species	1221:1227	arg1	B1A					1188:1190	B1A	1188:1190	B1A	1188:1190	We found two closely related MAGs (B1A and B1B) from one responsive species that both contain a polysaccharide utilization locus with a predicted extracellular α-amylase.
34851167	7	57	from	species	1221:1227	arg1	MAGs					1182:1185	two closely related MAGs	1162:1185	two closely related MAGs (B1A and B1B) from one responsive species that both contain a polysaccharide utilization locus with a predicted extracellular α-amylase	1162:1321	We found two closely related MAGs (B1A and B1B) from one responsive species that both contain a polysaccharide utilization locus with a predicted extracellular α-amylase.
34851167	12	58	theme	dramatic	2004:2011	arg1	changes					2013:2019	dramatic changes	2004:2019	dramatic changes in the abundance of some members of the gut microbiome	2004:2074	IMPORTANCE The drug acarbose is used to treat diabetes by preventing the breakdown of starch in the small intestine, resulting in dramatic changes in the abundance of some members of the gut microbiome and its fermentation products.
34851167	17	59	theme	of	2734:2735	arg1	enzyme					2737:2742	one type of enzyme	2725:2742	one type of enzyme known to participate in starch breakdown	2725:2783	In addition, genes encoding one type of enzyme known to participate in starch breakdown were found in all three genomes from responding species but none of the other genomes.
34851167	13	60	theme	primary	2255:2261	arg1	product					2276:2282	a primary fermentation product	2253:2282	a primary fermentation product	2253:2282	In mice, several of the bacteria that respond most positively are classified in the family Muribaculaceae, members of which produce propionate as a primary fermentation product.
34851167	13	60	theme	primary	2255:2261	arg1	propionate					2239:2248	propionate	2239:2248	propionate	2239:2248	In mice, several of the bacteria that respond most positively are classified in the family Muribaculaceae, members of which produce propionate as a primary fermentation product.
34851167	3	61	theme	fatty	511:515	arg1	propionate					523:532	propionate	523:532	propionate	523:532	Acarbose also increases longevity in mice, an effect that has been correlated with increased production of the short-chain fatty acids propionate and butyrate.
34851167	3	61	theme	fatty	511:515	arg1	acids					517:521	the short-chain fatty acids propionate and butyrate	495:545	the short-chain fatty acids propionate and butyrate	495:545	Acarbose also increases longevity in mice, an effect that has been correlated with increased production of the short-chain fatty acids propionate and butyrate.
34851167	3	61	theme	fatty	511:515	arg1	butyrate					538:545	butyrate	538:545	butyrate	538:545	Acarbose also increases longevity in mice, an effect that has been correlated with increased production of the short-chain fatty acids propionate and butyrate.
34851167	12	62	from	changes	2013:2019	arg1	abundance					2028:2036	the abundance	2024:2036	the abundance of some members of the gut microbiome	2024:2074	IMPORTANCE The drug acarbose is used to treat diabetes by preventing the breakdown of starch in the small intestine, resulting in dramatic changes in the abundance of some members of the gut microbiome and its fermentation products.
34851167	4	63	theme	bacterial	637:645	arg1	Muribaculaceae					654:667	the bacterial family Muribaculaceae	633:667	the bacterial family Muribaculaceae	633:667	In experiments replicated across three study sites, two distantly related species in the bacterial family Muribaculaceae were dramatically more abundant in acarbose-treated mice, distinguishing these responders from other members of the family.
34851167	5	64	theme	end	885:887	arg1	propionate					856:865	propionate	856:865	propionate	856:865	Bacteria in the family Muribaculaceae are predicted to produce propionate as a fermentation end product and are abundant and diverse in the guts of mice, although few isolates are available.
34851167	5	64	theme	end	885:887	arg1	product					889:895	a fermentation end product	870:895	a fermentation end product	870:895	Bacteria in the family Muribaculaceae are predicted to produce propionate as a fermentation end product and are abundant and diverse in the guts of mice, although few isolates are available.
34851167	16	65	theme	ecological	2643:2652	arg1	niches					2654:2659	the ecological niches	2639:2659	the ecological niches occupied by members of this family	2639:2694	Comparisons among genomes enhance existing models for the ecological niches occupied by members of this family.
34851167	4	66	theme	related	614:620	arg1	species					622:628	two distantly related species	600:628	two distantly related species in the bacterial family Muribaculaceae	600:667	In experiments replicated across three study sites, two distantly related species in the bacterial family Muribaculaceae were dramatically more abundant in acarbose-treated mice, distinguishing these responders from other members of the family.
34851167	10	67	theme	inconsistent	1634:1645	arg1	response					1647:1654	the inconsistent response	1630:1654	the inconsistent response of this species to acarbose across study sites	1630:1701	Differential gene content in B1A and B1B may be associated with the inconsistent response of this species to acarbose across study sites.
34851167	11	68	theme	bacteria	1806:1813	arg1	response					1832:1839	their response	1826:1839	their response to pharmaceutical perturbations	1826:1871	This work demonstrates the utility of culture-free genomics for inferring the ecological roles of gut bacteria, including their response to pharmaceutical perturbations.
34851167	11	68	theme	bacteria	1806:1813	arg1	roles					1793:1797	the ecological roles	1778:1797	the ecological roles	1778:1797	This work demonstrates the utility of culture-free genomics for inferring the ecological roles of gut bacteria, including their response to pharmaceutical perturbations.
34851167	12	69	theme	starch	1960:1965	arg1	breakdown					1947:1955	the breakdown	1943:1955	the breakdown of starch in the small intestine	1943:1988	IMPORTANCE The drug acarbose is used to treat diabetes by preventing the breakdown of starch in the small intestine, resulting in dramatic changes in the abundance of some members of the gut microbiome and its fermentation products.
34851167	8	70	theme	responsive	1453:1462	arg1	species					1464:1470	the only other responsive species	1438:1470	the only other responsive species	1438:1470	These genomes also shared a periplasmic neopullulanase with another, distantly related MAG (B2) representative of the only other responsive species.
34851167	11	71	theme	pharmaceutical	1844:1857	arg1	perturbations					1859:1871	pharmaceutical perturbations	1844:1871	pharmaceutical perturbations	1844:1871	This work demonstrates the utility of culture-free genomics for inferring the ecological roles of gut bacteria, including their response to pharmaceutical perturbations.
34851167	13	72	theme	bacteria	2131:2138	arg1	several					2116:2122	several	2116:2122	several	2116:2122	In mice, several of the bacteria that respond most positively are classified in the family Muribaculaceae, members of which produce propionate as a primary fermentation product.
34851167	4	73	theme	acarbose-treated	704:719	arg1	mice					721:724	acarbose-treated mice	704:724	acarbose-treated mice	704:724	In experiments replicated across three study sites, two distantly related species in the bacterial family Muribaculaceae were dramatically more abundant in acarbose-treated mice, distinguishing these responders from other members of the family.
34851167	1	74	from	α-amylase	193:201	arg1	intestine					216:224	the small intestine	206:224	the small intestine	206:224	The drug acarbose is used to treat diabetes and, by inhibiting α-amylase in the small intestine, increases the amount of starch entering the lower digestive tract.
34851167	1	75	theme	drug	134:137	arg1	acarbose					139:146	The drug acarbose	130:146	The drug acarbose	130:146	The drug acarbose is used to treat diabetes and, by inhibiting α-amylase in the small intestine, increases the amount of starch entering the lower digestive tract.
34851167	0	76	from	Treatment	111:119	arg1	Mice					124:127	Mice	124:127	Mice	124:127	Muribaculaceae Genomes Assembled from Metagenomes Suggest Genetic Drivers of Differential Response to Acarbose Treatment in Mice.
34851167	9	77	theme	nonresponding	1543:1555	arg1	species					1557:1563	nonresponding species	1543:1563	nonresponding species	1543:1563	This gene differentiated these three MAGs from MAGs representative of nonresponding species.
34851167	12	78	theme	fermentation	2084:2095	arg1	products					2097:2104	its fermentation products	2080:2104	its fermentation products	2080:2104	IMPORTANCE The drug acarbose is used to treat diabetes by preventing the breakdown of starch in the small intestine, resulting in dramatic changes in the abundance of some members of the gut microbiome and its fermentation products.
34851167	0	79	theme	Differential	77:88	arg1	Response					90:97	Differential Response	77:97	Differential Response to Acarbose Treatment in Mice	77:127	Muribaculaceae Genomes Assembled from Metagenomes Suggest Genetic Drivers of Differential Response to Acarbose Treatment in Mice.
34851167	14	80	theme	gut	2321:2323	arg1	health					2325:2330	gut health	2321:2330	gut health	2321:2330	Propionate has been associated with gut health and increased longevity in mice.
34851167	11	81	theme	genomics	1755:1762	arg1	utility					1731:1737	the utility	1727:1737	the utility of culture-free genomics for inferring the ecological roles of gut bacteria, including their response to pharmaceutical perturbations	1727:1871	This work demonstrates the utility of culture-free genomics for inferring the ecological roles of gut bacteria, including their response to pharmaceutical perturbations.
34851167	15	82	theme	specialization	2441:2454	arg1	signs					2432:2436	signs	2432:2436	signs of specialization	2432:2454	We found that genomes of the most responsive Muribaculaceae showed signs of specialization for starch fermentation, presumably providing them a competitive advantage in the large intestine of animals consuming acarbose.
34851167	15	83	theme	starch	2460:2465	arg1	fermentation					2467:2478	starch fermentation	2460:2478	starch fermentation	2460:2478	We found that genomes of the most responsive Muribaculaceae showed signs of specialization for starch fermentation, presumably providing them a competitive advantage in the large intestine of animals consuming acarbose.
34851167	9	84	theme	representative	1525:1538	arg1	MAGs					1520:1523	MAGs	1520:1523	MAGs representative of nonresponding species	1520:1563	This gene differentiated these three MAGs from MAGs representative of nonresponding species.
34851167	12	85	theme	gut	2061:2063	arg1	microbiome					2065:2074	the gut microbiome	2057:2074	the gut microbiome	2057:2074	IMPORTANCE The drug acarbose is used to treat diabetes by preventing the breakdown of starch in the small intestine, resulting in dramatic changes in the abundance of some members of the gut microbiome and its fermentation products.
34851167	5	86	from	Bacteria	793:800	arg1	Muribaculaceae					816:829	the family Muribaculaceae	805:829	the family Muribaculaceae	805:829	Bacteria in the family Muribaculaceae are predicted to produce propionate as a fermentation end product and are abundant and diverse in the guts of mice, although few isolates are available.
34851167	15	87	theme	animals	2557:2563	arg1	intestine					2544:2552	the large intestine	2534:2552	the large intestine of animals consuming acarbose	2534:2582	We found that genomes of the most responsive Muribaculaceae showed signs of specialization for starch fermentation, presumably providing them a competitive advantage in the large intestine of animals consuming acarbose.
34851167	5	88	theme	few	956:958	arg1	isolates					960:967	few isolates	956:967	few isolates	956:967	Bacteria in the family Muribaculaceae are predicted to produce propionate as a fermentation end product and are abundant and diverse in the guts of mice, although few isolates are available.
34851167	16	89	theme	existing	2619:2626	arg1	models					2628:2633	existing models	2619:2633	existing models for the ecological niches occupied by members of this family	2619:2694	Comparisons among genomes enhance existing models for the ecological niches occupied by members of this family.
34851167	7	90	dep	MAGs	1182:1185	arg1	B1B					1196:1198	B1B	1196:1198	B1B	1196:1198	We found two closely related MAGs (B1A and B1B) from one responsive species that both contain a polysaccharide utilization locus with a predicted extracellular α-amylase.
34851167	7	90	dep	MAGs	1182:1185	arg1	B1A					1188:1190	B1A	1188:1190	B1A	1188:1190	We found two closely related MAGs (B1A and B1B) from one responsive species that both contain a polysaccharide utilization locus with a predicted extracellular α-amylase.
34851167	7	90	dep	MAGs	1182:1185	arg1	MAGs					1182:1185	two closely related MAGs	1162:1185	two closely related MAGs (B1A and B1B) from one responsive species that both contain a polysaccharide utilization locus with a predicted extracellular α-amylase	1162:1321	We found two closely related MAGs (B1A and B1B) from one responsive species that both contain a polysaccharide utilization locus with a predicted extracellular α-amylase.
34851167	8	91	with	neopullulanase	1364:1377	arg1	another					1384:1390	another	1384:1390	another	1384:1390	These genomes also shared a periplasmic neopullulanase with another, distantly related MAG (B2) representative of the only other responsive species.
34851167	15	92	theme	Muribaculaceae	2410:2423	arg1	genomes					2379:2385	genomes	2379:2385	genomes of the most responsive Muribaculaceae	2379:2423	We found that genomes of the most responsive Muribaculaceae showed signs of specialization for starch fermentation, presumably providing them a competitive advantage in the large intestine of animals consuming acarbose.
34851167	11	93	theme	ecological	1782:1791	arg1	response					1832:1839	their response	1826:1839	their response to pharmaceutical perturbations	1826:1871	This work demonstrates the utility of culture-free genomics for inferring the ecological roles of gut bacteria, including their response to pharmaceutical perturbations.
34851167	11	93	theme	ecological	1782:1791	arg1	roles					1793:1797	the ecological roles	1778:1797	the ecological roles	1778:1797	This work demonstrates the utility of culture-free genomics for inferring the ecological roles of gut bacteria, including their response to pharmaceutical perturbations.
34851167	15	94	dep	them	2502:2505	arg1	advantage					2521:2529	a competitive advantage	2507:2529	them a competitive advantage in the large intestine of animals consuming acarbose	2502:2582	We found that genomes of the most responsive Muribaculaceae showed signs of specialization for starch fermentation, presumably providing them a competitive advantage in the large intestine of animals consuming acarbose.
34851167	2	95	theme	fermentation	365:376	arg1	products					378:385	their fermentation products	359:385	their fermentation products	359:385	This results in changes to the composition of the microbiota and their fermentation products.
34851167	5	96	theme	family	809:814	arg1	Muribaculaceae					816:829	the family Muribaculaceae	805:829	the family Muribaculaceae	805:829	Bacteria in the family Muribaculaceae are predicted to produce propionate as a fermentation end product and are abundant and diverse in the guts of mice, although few isolates are available.
34851167	5	97	from	guts	933:936	arg1	abundant					905:912	abundant	905:912	abundant	905:912	Bacteria in the family Muribaculaceae are predicted to produce propionate as a fermentation end product and are abundant and diverse in the guts of mice, although few isolates are available.
34851167	6	98	theme	Muribaculaceae	1057:1070	arg1	populations					1042:1052	nine populations	1037:1052	nine populations of Muribaculaceae	1037:1070	We reconstructed genomes from metagenomes (MAGs) for nine populations of Muribaculaceae to examine factors that distinguish species that respond positively to acarbose.
34851167	17	99	theme	starch	2768:2773	arg1	breakdown					2775:2783	starch breakdown	2768:2783	starch breakdown	2768:2783	In addition, genes encoding one type of enzyme known to participate in starch breakdown were found in all three genomes from responding species but none of the other genomes.
34851167	1	100	dep	treat	159:163	arg1	increases					227:235	increases	227:235	increases the amount of starch entering the lower digestive tract	227:291	The drug acarbose is used to treat diabetes and, by inhibiting α-amylase in the small intestine, increases the amount of starch entering the lower digestive tract.
34851167	4	101	dep	mice	721:724	arg1	distinguishing					727:740	distinguishing	727:740	distinguishing these responders from other members of the family	727:790	In experiments replicated across three study sites, two distantly related species in the bacterial family Muribaculaceae were dramatically more abundant in acarbose-treated mice, distinguishing these responders from other members of the family.
34851167	7	102	theme	predicted	1289:1297	arg1	α-amylase					1313:1321	a predicted extracellular α-amylase	1287:1321	a predicted extracellular α-amylase	1287:1321	We found two closely related MAGs (B1A and B1B) from one responsive species that both contain a polysaccharide utilization locus with a predicted extracellular α-amylase.
34851167	4	103	theme	family	785:790	arg1	members					770:776	other members	764:776	other members of the family	764:790	In experiments replicated across three study sites, two distantly related species in the bacterial family Muribaculaceae were dramatically more abundant in acarbose-treated mice, distinguishing these responders from other members of the family.
34851167	1	104	theme	digestive	277:285	arg1	tract					287:291	the lower digestive tract	267:291	the lower digestive tract	267:291	The drug acarbose is used to treat diabetes and, by inhibiting α-amylase in the small intestine, increases the amount of starch entering the lower digestive tract.
34851167	17	105	from	none	2845:2848	arg1	genomes					2809:2815	all three genomes	2799:2815	all three genomes from responding species but none of the other genomes	2799:2869	In addition, genes encoding one type of enzyme known to participate in starch breakdown were found in all three genomes from responding species but none of the other genomes.
34851167	7	106	theme	utilization	1264:1274	arg1	locus					1276:1280	a polysaccharide utilization locus	1247:1280	a polysaccharide utilization locus	1247:1280	We found two closely related MAGs (B1A and B1B) from one responsive species that both contain a polysaccharide utilization locus with a predicted extracellular α-amylase.
34851167	8	107	theme	MAG	1411:1413	arg1	representative					1420:1433	distantly related MAG (B2) representative	1393:1433	distantly related MAG (B2) representative of the only other responsive species	1393:1470	These genomes also shared a periplasmic neopullulanase with another, distantly related MAG (B2) representative of the only other responsive species.
34851167	8	107	theme	MAG	1411:1413	arg1	neopullulanase					1364:1377	a periplasmic neopullulanase	1350:1377	a periplasmic neopullulanase with another	1350:1390	These genomes also shared a periplasmic neopullulanase with another, distantly related MAG (B2) representative of the only other responsive species.
34851167	3	108	theme	increased	471:479	arg1	production					481:490	increased production	471:490	increased production of the short-chain fatty acids propionate and butyrate	471:545	Acarbose also increases longevity in mice, an effect that has been correlated with increased production of the short-chain fatty acids propionate and butyrate.
34851167	17	109	from	species	2833:2839	arg1	genomes					2809:2815	all three genomes	2799:2815	all three genomes from responding species but none of the other genomes	2799:2869	In addition, genes encoding one type of enzyme known to participate in starch breakdown were found in all three genomes from responding species but none of the other genomes.
34851167	14	110	from	longevity	2346:2354	arg1	mice					2359:2362	mice	2359:2362	mice	2359:2362	Propionate has been associated with gut health and increased longevity in mice.
34851167	8	111	theme	B2	1416:1417	arg1	representative					1420:1433	distantly related MAG (B2) representative	1393:1433	distantly related MAG (B2) representative of the only other responsive species	1393:1470	These genomes also shared a periplasmic neopullulanase with another, distantly related MAG (B2) representative of the only other responsive species.
34851167	8	111	theme	B2	1416:1417	arg1	neopullulanase					1364:1377	a periplasmic neopullulanase	1350:1377	a periplasmic neopullulanase with another	1350:1390	These genomes also shared a periplasmic neopullulanase with another, distantly related MAG (B2) representative of the only other responsive species.
34851167	3	112	theme	short-chain	499:509	arg1	propionate					523:532	propionate	523:532	propionate	523:532	Acarbose also increases longevity in mice, an effect that has been correlated with increased production of the short-chain fatty acids propionate and butyrate.
34851167	3	112	theme	short-chain	499:509	arg1	acids					517:521	the short-chain fatty acids propionate and butyrate	495:545	the short-chain fatty acids propionate and butyrate	495:545	Acarbose also increases longevity in mice, an effect that has been correlated with increased production of the short-chain fatty acids propionate and butyrate.
34851167	3	112	theme	short-chain	499:509	arg1	butyrate					538:545	butyrate	538:545	butyrate	538:545	Acarbose also increases longevity in mice, an effect that has been correlated with increased production of the short-chain fatty acids propionate and butyrate.
34851167	4	113	from	mice	721:724	arg1	abundant					692:699	abundant	692:699	abundant	692:699	In experiments replicated across three study sites, two distantly related species in the bacterial family Muribaculaceae were dramatically more abundant in acarbose-treated mice, distinguishing these responders from other members of the family.
34851167	12	114	dep	IMPORTANCE	1874:1883	arg1	acarbose					1894:1901	The drug acarbose	1885:1901	IMPORTANCE The drug acarbose	1874:1901	IMPORTANCE The drug acarbose is used to treat diabetes by preventing the breakdown of starch in the small intestine, resulting in dramatic changes in the abundance of some members of the gut microbiome and its fermentation products.
34851167	4	115	from	experiments	551:561	arg1	abundant					692:699	abundant	692:699	abundant	692:699	In experiments replicated across three study sites, two distantly related species in the bacterial family Muribaculaceae were dramatically more abundant in acarbose-treated mice, distinguishing these responders from other members of the family.
32518945	5	0	from	success	1014:1020	arg1	environment					1033:1043	the gut environment	1025:1043	the gut environment	1025:1043	The ability of the Bacteroidetes to target highly sulfated host glycans is key to their success in the gut environment but can lead to inflammation in susceptible hosts.
32518945	5	1	theme	gut	1029:1031	arg1	environment					1033:1043	the gut environment	1025:1043	the gut environment	1025:1043	The ability of the Bacteroidetes to target highly sulfated host glycans is key to their success in the gut environment but can lead to inflammation in susceptible hosts.
32518945	2	2	link	N-linked	334:341	arg1	sulfate					441:447	chondroitin sulfate	429:447	chondroitin sulfate (CS)	429:452	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	2	2	link	N-linked	334:341	arg1	heparin					411:417	heparin	411:417	heparin (Hep)	411:423	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	2	2	link	N-linked	334:341	arg1	glycoproteins					343:355	N-linked glycoproteins	334:355	N-linked glycoproteins	334:355	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	6	3	theme	novel	1246:1250	arg1	ways					1252:1255	novel ways	1246:1255	novel ways	1246:1255	Therefore, our continued understanding of the molecular strategies employed by these bacteria to scavenge carbohydrate nutrition is likely to lead to novel ways to alter their metabolism to promote host health.
32518945	6	4	theme	strategies	1152:1161	arg1	understanding					1121:1133	our continued understanding	1107:1133	our continued understanding of the molecular strategies employed by these bacteria to scavenge carbohydrate nutrition	1107:1223	Therefore, our continued understanding of the molecular strategies employed by these bacteria to scavenge carbohydrate nutrition is likely to lead to novel ways to alter their metabolism to promote host health.
32518945	4	5	theme	/hyaluronic	865:875	arg1	HA					883:884	HA	883:884	HA	883:884	Here we discuss the Sus-like paradigm that defines glycan uptake by the Bacteroidetes and the salient details of the PULs that target heparin/heparan sulfate (HS) and chondroitin sulfate/dermatan sulfate (DS)/hyaluronic acid (HA), respectively, in B. thetaiotaomicron.
32518945	4	5	theme	/hyaluronic	865:875	arg1	acid					877:880	/hyaluronic acid	865:880	/hyaluronic acid (HA)	865:885	Here we discuss the Sus-like paradigm that defines glycan uptake by the Bacteroidetes and the salient details of the PULs that target heparin/heparan sulfate (HS) and chondroitin sulfate/dermatan sulfate (DS)/hyaluronic acid (HA), respectively, in B. thetaiotaomicron.
32518945	0	6	theme	Host	0:3	arg1	utilization					12:22	Host glycan utilization	0:22	Host glycan utilization within the Bacteroidetes Sus-like paradigm.	0:66	Host glycan utilization within the Bacteroidetes Sus-like paradigm.
32518945	3	7	theme	recent	463:468	arg1	studies					482:488	Several recent biochemical studies	455:488	Several recent biochemical studies	455:488	Several recent biochemical studies have revealed the specific polysaccharide utilization loci (PULs) within the model symbiont Bacteroides thetaiotaomicron for the deconstruction of these host glycans.
32518945	4	8	theme	sulfate/dermatan	836:851	arg1	sulfate					853:859	chondroitin sulfate/dermatan sulfate	824:859	chondroitin sulfate/dermatan sulfate (DS)	824:864	Here we discuss the Sus-like paradigm that defines glycan uptake by the Bacteroidetes and the salient details of the PULs that target heparin/heparan sulfate (HS) and chondroitin sulfate/dermatan sulfate (DS)/hyaluronic acid (HA), respectively, in B. thetaiotaomicron.
32518945	4	8	theme	sulfate/dermatan	836:851	arg1	DS					862:863	DS	862:863	DS	862:863	Here we discuss the Sus-like paradigm that defines glycan uptake by the Bacteroidetes and the salient details of the PULs that target heparin/heparan sulfate (HS) and chondroitin sulfate/dermatan sulfate (DS)/hyaluronic acid (HA), respectively, in B. thetaiotaomicron.
32518945	3	9	theme	polysaccharide	517:530	arg1	loci					544:547	the specific polysaccharide utilization loci	504:547	the specific polysaccharide utilization loci (PULs) within the model symbiont Bacteroides thetaiotaomicron for the deconstruction of these host glycans	504:654	Several recent biochemical studies have revealed the specific polysaccharide utilization loci (PULs) within the model symbiont Bacteroides thetaiotaomicron for the deconstruction of these host glycans.
32518945	3	9	theme	polysaccharide	517:530	arg1	PULs					550:553	PULs	550:553	PULs	550:553	Several recent biochemical studies have revealed the specific polysaccharide utilization loci (PULs) within the model symbiont Bacteroides thetaiotaomicron for the deconstruction of these host glycans.
32518945	1	10	theme	expanded	174:181	arg1	capacity					183:190	a greatly expanded capacity	164:190	a greatly expanded capacity to degrade complex glycans	164:217	The Bacteroidetes are numerically abundant Gram-negative organisms of the distal human gut with a greatly expanded capacity to degrade complex glycans.
32518945	4	11	theme	chondroitin	824:834	arg1	sulfate					853:859	chondroitin sulfate/dermatan sulfate	824:859	chondroitin sulfate/dermatan sulfate (DS)	824:864	Here we discuss the Sus-like paradigm that defines glycan uptake by the Bacteroidetes and the salient details of the PULs that target heparin/heparan sulfate (HS) and chondroitin sulfate/dermatan sulfate (DS)/hyaluronic acid (HA), respectively, in B. thetaiotaomicron.
32518945	4	11	theme	chondroitin	824:834	arg1	DS					862:863	DS	862:863	DS	862:863	Here we discuss the Sus-like paradigm that defines glycan uptake by the Bacteroidetes and the salient details of the PULs that target heparin/heparan sulfate (HS) and chondroitin sulfate/dermatan sulfate (DS)/hyaluronic acid (HA), respectively, in B. thetaiotaomicron.
32518945	1	12	with	organisms	125:133	arg1	capacity					183:190	a greatly expanded capacity	164:190	a greatly expanded capacity to degrade complex glycans	164:217	The Bacteroidetes are numerically abundant Gram-negative organisms of the distal human gut with a greatly expanded capacity to degrade complex glycans.
32518945	6	13	theme	molecular	1142:1150	arg1	strategies					1152:1161	the molecular strategies	1138:1161	the molecular strategies employed by these bacteria to scavenge carbohydrate nutrition	1138:1223	Therefore, our continued understanding of the molecular strategies employed by these bacteria to scavenge carbohydrate nutrition is likely to lead to novel ways to alter their metabolism to promote host health.
32518945	0	14	theme	glycan	5:10	arg1	utilization					12:22	Host glycan utilization	0:22	Host glycan utilization within the Bacteroidetes Sus-like paradigm.	0:66	Host glycan utilization within the Bacteroidetes Sus-like paradigm.
32518945	3	15	theme	specific	508:515	arg1	loci					544:547	the specific polysaccharide utilization loci	504:547	the specific polysaccharide utilization loci (PULs) within the model symbiont Bacteroides thetaiotaomicron for the deconstruction of these host glycans	504:654	Several recent biochemical studies have revealed the specific polysaccharide utilization loci (PULs) within the model symbiont Bacteroides thetaiotaomicron for the deconstruction of these host glycans.
32518945	3	15	theme	specific	508:515	arg1	PULs					550:553	PULs	550:553	PULs	550:553	Several recent biochemical studies have revealed the specific polysaccharide utilization loci (PULs) within the model symbiont Bacteroides thetaiotaomicron for the deconstruction of these host glycans.
32518945	2	16	theme	O-linked	316:323	arg1	heparin					411:417	heparin	411:417	heparin (Hep)	411:423	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	2	16	theme	O-linked	316:323	arg1	glycans					325:331	mucin O-linked glycans	310:331	mucin O-linked glycans	310:331	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	2	16	theme	O-linked	316:323	arg1	sulfate					441:447	chondroitin sulfate	429:447	chondroitin sulfate (CS)	429:452	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	2	17	theme	chondroitin	429:439	arg1	CS					450:451	CS	450:451	CS	450:451	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	2	17	theme	chondroitin	429:439	arg1	sulfate					441:447	chondroitin sulfate	429:447	chondroitin sulfate (CS)	429:452	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	6	18	theme	carbohydrate	1202:1213	arg1	nutrition					1215:1223	carbohydrate nutrition	1202:1223	carbohydrate nutrition	1202:1223	Therefore, our continued understanding of the molecular strategies employed by these bacteria to scavenge carbohydrate nutrition is likely to lead to novel ways to alter their metabolism to promote host health.
32518945	1	19	theme	abundant	102:109	arg1	Bacteroidetes					72:84	The Bacteroidetes	68:84	The Bacteroidetes	68:84	The Bacteroidetes are numerically abundant Gram-negative organisms of the distal human gut with a greatly expanded capacity to degrade complex glycans.
32518945	1	19	theme	abundant	102:109	arg1	organisms					125:133	numerically abundant Gram-negative organisms	90:133	numerically abundant Gram-negative organisms of the distal human gut	90:157	The Bacteroidetes are numerically abundant Gram-negative organisms of the distal human gut with a greatly expanded capacity to degrade complex glycans.
32518945	1	20	theme	Gram-negative	111:123	arg1	Bacteroidetes					72:84	The Bacteroidetes	68:84	The Bacteroidetes	68:84	The Bacteroidetes are numerically abundant Gram-negative organisms of the distal human gut with a greatly expanded capacity to degrade complex glycans.
32518945	1	20	theme	Gram-negative	111:123	arg1	organisms					125:133	numerically abundant Gram-negative organisms	90:133	numerically abundant Gram-negative organisms of the distal human gut	90:157	The Bacteroidetes are numerically abundant Gram-negative organisms of the distal human gut with a greatly expanded capacity to degrade complex glycans.
32518945	1	21	theme	complex	203:209	arg1	glycans					211:217	complex glycans	203:217	complex glycans	203:217	The Bacteroidetes are numerically abundant Gram-negative organisms of the distal human gut with a greatly expanded capacity to degrade complex glycans.
32518945	2	22	theme	mucin	310:314	arg1	heparin					411:417	heparin	411:417	heparin (Hep)	411:423	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	2	22	theme	mucin	310:314	arg1	glycans					325:331	mucin O-linked glycans	310:331	mucin O-linked glycans	310:331	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	2	22	theme	mucin	310:314	arg1	sulfate					441:447	chondroitin sulfate	429:447	chondroitin sulfate (CS)	429:452	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	4	23	theme	PULs	774:777	arg1	Bacteroidetes					729:741	the Bacteroidetes	725:741	the Bacteroidetes	725:741	Here we discuss the Sus-like paradigm that defines glycan uptake by the Bacteroidetes and the salient details of the PULs that target heparin/heparan sulfate (HS) and chondroitin sulfate/dermatan sulfate (DS)/hyaluronic acid (HA), respectively, in B. thetaiotaomicron.
32518945	4	23	theme	PULs	774:777	arg1	details					759:765	the salient details	747:765	the salient details of the PULs that target heparin/heparan sulfate (HS) and chondroitin sulfate/dermatan sulfate (DS)/hyaluronic acid (HA), respectively, in B. thetaiotaomicron	747:923	Here we discuss the Sus-like paradigm that defines glycan uptake by the Bacteroidetes and the salient details of the PULs that target heparin/heparan sulfate (HS) and chondroitin sulfate/dermatan sulfate (DS)/hyaluronic acid (HA), respectively, in B. thetaiotaomicron.
32518945	3	24	theme	glycans	648:654	arg1	deconstruction					619:632	the deconstruction	615:632	the deconstruction of these host glycans	615:654	Several recent biochemical studies have revealed the specific polysaccharide utilization loci (PULs) within the model symbiont Bacteroides thetaiotaomicron for the deconstruction of these host glycans.
32518945	6	25	theme	continued	1111:1119	arg1	understanding					1121:1133	our continued understanding	1107:1133	our continued understanding of the molecular strategies employed by these bacteria to scavenge carbohydrate nutrition	1107:1223	Therefore, our continued understanding of the molecular strategies employed by these bacteria to scavenge carbohydrate nutrition is likely to lead to novel ways to alter their metabolism to promote host health.
32518945	0	26	theme	Bacteroidetes	35:47	arg1	paradigm					58:65	the Bacteroidetes Sus-like paradigm	31:65	the Bacteroidetes Sus-like paradigm	31:65	Host glycan utilization within the Bacteroidetes Sus-like paradigm.
32518945	4	27	theme	glycan	708:713	arg1	uptake					715:720	glycan uptake	708:720	glycan uptake	708:720	Here we discuss the Sus-like paradigm that defines glycan uptake by the Bacteroidetes and the salient details of the PULs that target heparin/heparan sulfate (HS) and chondroitin sulfate/dermatan sulfate (DS)/hyaluronic acid (HA), respectively, in B. thetaiotaomicron.
32518945	4	28	theme	B.	905:906	arg1	thetaiotaomicron					908:923	B. thetaiotaomicron	905:923	B. thetaiotaomicron	905:923	Here we discuss the Sus-like paradigm that defines glycan uptake by the Bacteroidetes and the salient details of the PULs that target heparin/heparan sulfate (HS) and chondroitin sulfate/dermatan sulfate (DS)/hyaluronic acid (HA), respectively, in B. thetaiotaomicron.
32518945	3	29	theme	Bacteroides	582:592	arg1	thetaiotaomicron					594:609	the model symbiont Bacteroides thetaiotaomicron	563:609	the model symbiont Bacteroides thetaiotaomicron for the deconstruction of these host glycans	563:654	Several recent biochemical studies have revealed the specific polysaccharide utilization loci (PULs) within the model symbiont Bacteroides thetaiotaomicron for the deconstruction of these host glycans.
32518945	4	30	dep	sulfate	807:813	arg1	HA					883:884	HA	883:884	HA	883:884	Here we discuss the Sus-like paradigm that defines glycan uptake by the Bacteroidetes and the salient details of the PULs that target heparin/heparan sulfate (HS) and chondroitin sulfate/dermatan sulfate (DS)/hyaluronic acid (HA), respectively, in B. thetaiotaomicron.
32518945	4	30	dep	sulfate	807:813	arg1	acid					877:880	/hyaluronic acid	865:880	/hyaluronic acid (HA)	865:885	Here we discuss the Sus-like paradigm that defines glycan uptake by the Bacteroidetes and the salient details of the PULs that target heparin/heparan sulfate (HS) and chondroitin sulfate/dermatan sulfate (DS)/hyaluronic acid (HA), respectively, in B. thetaiotaomicron.
32518945	6	31	theme	host	1294:1297	arg1	health					1299:1304	host health	1294:1304	host health	1294:1304	Therefore, our continued understanding of the molecular strategies employed by these bacteria to scavenge carbohydrate nutrition is likely to lead to novel ways to alter their metabolism to promote host health.
32518945	2	32	theme	these	232:236	arg1	subset					222:227	A subset	220:227	A subset of these	220:236	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	2	32	theme	these	232:236	arg1	adept					242:246	adept	242:246	adept	242:246	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	4	33	theme	salient	751:757	arg1	details					759:765	the salient details	747:765	the salient details of the PULs that target heparin/heparan sulfate (HS) and chondroitin sulfate/dermatan sulfate (DS)/hyaluronic acid (HA), respectively, in B. thetaiotaomicron	747:923	Here we discuss the Sus-like paradigm that defines glycan uptake by the Bacteroidetes and the salient details of the PULs that target heparin/heparan sulfate (HS) and chondroitin sulfate/dermatan sulfate (DS)/hyaluronic acid (HA), respectively, in B. thetaiotaomicron.
32518945	3	34	theme	host	643:646	arg1	glycans					648:654	these host glycans	637:654	these host glycans	637:654	Several recent biochemical studies have revealed the specific polysaccharide utilization loci (PULs) within the model symbiont Bacteroides thetaiotaomicron for the deconstruction of these host glycans.
32518945	3	35	theme	model	567:571	arg1	thetaiotaomicron					594:609	the model symbiont Bacteroides thetaiotaomicron	563:609	the model symbiont Bacteroides thetaiotaomicron for the deconstruction of these host glycans	563:654	Several recent biochemical studies have revealed the specific polysaccharide utilization loci (PULs) within the model symbiont Bacteroides thetaiotaomicron for the deconstruction of these host glycans.
32518945	3	36	theme	biochemical	470:480	arg1	studies					482:488	Several recent biochemical studies	455:488	Several recent biochemical studies	455:488	Several recent biochemical studies have revealed the specific polysaccharide utilization loci (PULs) within the model symbiont Bacteroides thetaiotaomicron for the deconstruction of these host glycans.
32518945	0	37	theme	Sus-like	49:56	arg1	paradigm					58:65	the Bacteroidetes Sus-like paradigm	31:65	the Bacteroidetes Sus-like paradigm	31:65	Host glycan utilization within the Bacteroidetes Sus-like paradigm.
32518945	2	38	link	O-linked	316:323	arg1	heparin					411:417	heparin	411:417	heparin (Hep)	411:423	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	2	38	link	O-linked	316:323	arg1	glycans					325:331	mucin O-linked glycans	310:331	mucin O-linked glycans	310:331	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	2	38	link	O-linked	316:323	arg1	sulfate					441:447	chondroitin sulfate	429:447	chondroitin sulfate (CS)	429:452	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	2	39	gly	glycoproteins	343:355	arg1	sulfate					441:447	chondroitin sulfate	429:447	chondroitin sulfate (CS)	429:452	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	2	39	gly	glycoproteins	343:355	arg1	heparin					411:417	heparin	411:417	heparin (Hep)	411:423	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	2	39	gly	glycoproteins	343:355	arg1	glycoproteins					343:355	N-linked glycoproteins	334:355	N-linked glycoproteins	334:355	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	3	40	theme	symbiont	573:580	arg1	thetaiotaomicron					594:609	the model symbiont Bacteroides thetaiotaomicron	563:609	the model symbiont Bacteroides thetaiotaomicron for the deconstruction of these host glycans	563:654	Several recent biochemical studies have revealed the specific polysaccharide utilization loci (PULs) within the model symbiont Bacteroides thetaiotaomicron for the deconstruction of these host glycans.
32518945	2	41	theme	N-linked	334:341	arg1	sulfate					441:447	chondroitin sulfate	429:447	chondroitin sulfate (CS)	429:452	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	2	41	theme	N-linked	334:341	arg1	heparin					411:417	heparin	411:417	heparin (Hep)	411:423	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	2	41	theme	N-linked	334:341	arg1	glycoproteins					343:355	N-linked glycoproteins	334:355	N-linked glycoproteins	334:355	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	5	42	from	inflammation	1061:1072	arg1	hosts					1089:1093	susceptible hosts	1077:1093	susceptible hosts	1077:1093	The ability of the Bacteroidetes to target highly sulfated host glycans is key to their success in the gut environment but can lead to inflammation in susceptible hosts.
32518945	5	43	theme	host	985:988	arg1	glycans					990:996	highly sulfated host glycans	969:996	highly sulfated host glycans	969:996	The ability of the Bacteroidetes to target highly sulfated host glycans is key to their success in the gut environment but can lead to inflammation in susceptible hosts.
32518945	2	44	theme	host	262:265	arg1	glycans					267:273	scavenging host glycans	251:273	scavenging host glycans	251:273	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	2	44	theme	host	262:265	arg1	glycoproteins					343:355	N-linked glycoproteins	334:355	N-linked glycoproteins	334:355	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	2	44	theme	host	262:265	arg1	glycans					325:331	mucin O-linked glycans	310:331	mucin O-linked glycans	310:331	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	2	44	theme	host	262:265	arg1	glycosaminoglycans					377:394	highly sulfated glycosaminoglycans	361:394	highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS)	361:452	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	4	45	theme	Sus-like	677:684	arg1	paradigm					686:693	the Sus-like paradigm	673:693	the Sus-like paradigm that defines glycan uptake by the Bacteroidetes and the salient details of the PULs that target heparin/heparan sulfate (HS) and chondroitin sulfate/dermatan sulfate (DS)/hyaluronic acid (HA), respectively, in B. thetaiotaomicron	673:923	Here we discuss the Sus-like paradigm that defines glycan uptake by the Bacteroidetes and the salient details of the PULs that target heparin/heparan sulfate (HS) and chondroitin sulfate/dermatan sulfate (DS)/hyaluronic acid (HA), respectively, in B. thetaiotaomicron.
32518945	1	46	theme	distal	142:147	arg1	gut					155:157	the distal human gut	138:157	the distal human gut	138:157	The Bacteroidetes are numerically abundant Gram-negative organisms of the distal human gut with a greatly expanded capacity to degrade complex glycans.
32518945	2	47	from	glycans	267:273	arg1	subset					222:227	A subset	220:227	A subset of these	220:236	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	2	47	from	glycans	267:273	arg1	adept					242:246	adept	242:246	adept	242:246	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	3	48	theme	utilization	532:542	arg1	loci					544:547	the specific polysaccharide utilization loci	504:547	the specific polysaccharide utilization loci (PULs) within the model symbiont Bacteroides thetaiotaomicron for the deconstruction of these host glycans	504:654	Several recent biochemical studies have revealed the specific polysaccharide utilization loci (PULs) within the model symbiont Bacteroides thetaiotaomicron for the deconstruction of these host glycans.
32518945	3	48	theme	utilization	532:542	arg1	PULs					550:553	PULs	550:553	PULs	550:553	Several recent biochemical studies have revealed the specific polysaccharide utilization loci (PULs) within the model symbiont Bacteroides thetaiotaomicron for the deconstruction of these host glycans.
32518945	2	49	theme	scavenging	251:260	arg1	glycans					267:273	scavenging host glycans	251:273	scavenging host glycans	251:273	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	2	49	theme	scavenging	251:260	arg1	glycoproteins					343:355	N-linked glycoproteins	334:355	N-linked glycoproteins	334:355	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	2	49	theme	scavenging	251:260	arg1	glycans					325:331	mucin O-linked glycans	310:331	mucin O-linked glycans	310:331	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	2	49	theme	scavenging	251:260	arg1	glycosaminoglycans					377:394	highly sulfated glycosaminoglycans	361:394	highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS)	361:452	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	1	50	theme	human	149:153	arg1	gut					155:157	the distal human gut	138:157	the distal human gut	138:157	The Bacteroidetes are numerically abundant Gram-negative organisms of the distal human gut with a greatly expanded capacity to degrade complex glycans.
32518945	3	51	theme	Several	455:461	arg1	studies					482:488	Several recent biochemical studies	455:488	Several recent biochemical studies	455:488	Several recent biochemical studies have revealed the specific polysaccharide utilization loci (PULs) within the model symbiont Bacteroides thetaiotaomicron for the deconstruction of these host glycans.
32518945	5	52	theme	Bacteroidetes	945:957	arg1	ability					930:936	The ability	926:936	The ability of the Bacteroidetes to target highly sulfated host glycans	926:996	The ability of the Bacteroidetes to target highly sulfated host glycans is key to their success in the gut environment but can lead to inflammation in susceptible hosts.
32518945	5	52	theme	Bacteroidetes	945:957	arg1	key					1001:1003	key	1001:1003	key	1001:1003	The ability of the Bacteroidetes to target highly sulfated host glycans is key to their success in the gut environment but can lead to inflammation in susceptible hosts.
32518945	5	53	theme	sulfated	976:983	arg1	glycans					990:996	highly sulfated host glycans	969:996	highly sulfated host glycans	969:996	The ability of the Bacteroidetes to target highly sulfated host glycans is key to their success in the gut environment but can lead to inflammation in susceptible hosts.
32518945	1	54	theme	gut	155:157	arg1	Bacteroidetes					72:84	The Bacteroidetes	68:84	The Bacteroidetes	68:84	The Bacteroidetes are numerically abundant Gram-negative organisms of the distal human gut with a greatly expanded capacity to degrade complex glycans.
32518945	1	54	theme	gut	155:157	arg1	organisms					125:133	numerically abundant Gram-negative organisms	90:133	numerically abundant Gram-negative organisms of the distal human gut	90:157	The Bacteroidetes are numerically abundant Gram-negative organisms of the distal human gut with a greatly expanded capacity to degrade complex glycans.
32518945	5	55	theme	susceptible	1077:1087	arg1	hosts					1089:1093	susceptible hosts	1077:1093	susceptible hosts	1077:1093	The ability of the Bacteroidetes to target highly sulfated host glycans is key to their success in the gut environment but can lead to inflammation in susceptible hosts.
32518945	4	56	theme	heparin/heparan	791:805	arg1	HS					816:817	HS	816:817	HS	816:817	Here we discuss the Sus-like paradigm that defines glycan uptake by the Bacteroidetes and the salient details of the PULs that target heparin/heparan sulfate (HS) and chondroitin sulfate/dermatan sulfate (DS)/hyaluronic acid (HA), respectively, in B. thetaiotaomicron.
32518945	4	56	theme	heparin/heparan	791:805	arg1	sulfate					807:813	heparin/heparan sulfate	791:813	heparin/heparan sulfate (HS)	791:818	Here we discuss the Sus-like paradigm that defines glycan uptake by the Bacteroidetes and the salient details of the PULs that target heparin/heparan sulfate (HS) and chondroitin sulfate/dermatan sulfate (DS)/hyaluronic acid (HA), respectively, in B. thetaiotaomicron.
32518945	2	57	theme	sulfated	368:375	arg1	heparin					411:417	heparin	411:417	heparin (Hep)	411:423	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	2	57	theme	sulfated	368:375	arg1	sulfate					441:447	chondroitin sulfate	429:447	chondroitin sulfate (CS)	429:452	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	2	57	theme	sulfated	368:375	arg1	GAGs					397:400	GAGs	397:400	GAGs	397:400	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
32518945	2	57	theme	sulfated	368:375	arg1	glycosaminoglycans					377:394	highly sulfated glycosaminoglycans	361:394	highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS)	361:452	A subset of these are adept at scavenging host glycans within this environment, including mucin O-linked glycans, N-linked glycoproteins and highly sulfated glycosaminoglycans (GAGs) such as heparin (Hep) and chondroitin sulfate (CS).
34280500	8	0	theme	parallel	1177:1184	arg1	experiment					1186:1195	two parallel experiment	1173:1195	two parallel experiment	1173:1195	Fresh fecal and colon samples were collected, then VRE colonization, gut microbiota and gene expression were respectively assessed using cultivation, 16s rRNA sequencing and RNA-sequencing in two parallel experiment, respectively.
34280500	5	1	theme	2 × 108 CFU	712:722	arg1	VRE					724:726	2 × 108 CFU VRE	712:726	2 × 108 CFU VRE	712:726	In VRE-infection experiment, 6-week old female C57/6 J mice pre-treated with vancomycin were infected with 2 × 108 CFU VRE via gavage.
34280500	11	2	theme	uninfected	1640:1649	arg1	P < 0.05					1656:1663	P < 0.05	1656:1663	P < 0.05	1656:1663	Uninfected mice treated with stachyose showed a significant increase in Lactobacillaceae and Lactobacillus compared to the PBS-treated uninfected mice(P < 0.05).
34280500	11	2	theme	uninfected	1640:1649	arg1	mice					1651:1654	the PBS-treated uninfected mice	1624:1654	the PBS-treated uninfected mice(P < 0.05)	1624:1664	Uninfected mice treated with stachyose showed a significant increase in Lactobacillaceae and Lactobacillus compared to the PBS-treated uninfected mice(P < 0.05).
34280500	8	3	theme	colon	997:1001	arg1	samples					1003:1009	Fresh fecal and colon samples	981:1009	samples	1003:1009	Fresh fecal and colon samples were collected, then VRE colonization, gut microbiota and gene expression were respectively assessed using cultivation, 16s rRNA sequencing and RNA-sequencing in two parallel experiment, respectively.
34280500	7	4	theme	PBS	905:907	arg1	gavage					882:887	daily gavage	876:887	daily gavage of stachyose or PBS for 7 days	876:918	Two groups of uninfected mice were also received daily gavage of stachyose or PBS for 7 days to observe the impact of stachyose treatment on normal mice.
34280500	9	5	theme	stachyose	1234:1242	arg1	treatment					1244:1252	stachyose treatment	1234:1252	stachyose treatment	1234:1252	In VRE-infected mice, stachyose treatment significantly reduced VRE colonization on days 9 and 10 post-infection.
34280500	7	6	theme	stachyose	892:900	arg1	gavage					882:887	daily gavage	876:887	daily gavage of stachyose or PBS for 7 days	876:918	Two groups of uninfected mice were also received daily gavage of stachyose or PBS for 7 days to observe the impact of stachyose treatment on normal mice.
34280500	2	7	dep	intervention	296:307	arg1	represents					309:318	represents	309:318	represents one of such effort	309:337	Multiple measures have been investigated to address this issue, altering gut microbiota through dietary intervention represents one of such effort.
34280500	12	8	theme	stachyose	1702:1710	arg1	treatment					1712:1720	stachyose treatment	1702:1720	stachyose treatment in VRE-infected mice	1702:1741	RNA-sequencing results showed that stachyose treatment in VRE-infected mice increased expression of genes involved in TNF and IL-17 signaling pathways.
34280500	8	9	theme	VRE	1032:1034	arg1	colonization					1036:1047	VRE colonization	1032:1047	VRE colonization	1032:1047	Fresh fecal and colon samples were collected, then VRE colonization, gut microbiota and gene expression were respectively assessed using cultivation, 16s rRNA sequencing and RNA-sequencing in two parallel experiment, respectively.
34280500	10	10	theme	infection	1478:1486	arg1	P < 0.01					1494:1501	P < 0.01	1494:1501	P < 0.01	1494:1501	Stachyose treatment increased the relative abundance of Porphyromonadaceae, Parabacteroides, and Parabacteroides distasonis compared to the PBS-treated infection mice (P < 0.01).
34280500	10	10	theme	infection	1478:1486	arg1	mice					1488:1491	the PBS-treated infection mice	1462:1491	the PBS-treated infection mice (P < 0.01)	1462:1502	Stachyose treatment increased the relative abundance of Porphyromonadaceae, Parabacteroides, and Parabacteroides distasonis compared to the PBS-treated infection mice (P < 0.01).
34280500	11	11	from	increase	1565:1572	arg1	Lactobacillus					1598:1610	Lactobacillus	1598:1610	Lactobacillus	1598:1610	Uninfected mice treated with stachyose showed a significant increase in Lactobacillaceae and Lactobacillus compared to the PBS-treated uninfected mice(P < 0.05).
34280500	11	11	from	increase	1565:1572	arg1	Lactobacillaceae					1577:1592	Lactobacillaceae	1577:1592	Lactobacillaceae	1577:1592	Uninfected mice treated with stachyose showed a significant increase in Lactobacillaceae and Lactobacillus compared to the PBS-treated uninfected mice(P < 0.05).
34280500	9	12	theme	VRE	1276:1278	arg1	colonization					1280:1291	VRE colonization	1276:1291	VRE colonization on days 9 and 10	1276:1308	In VRE-infected mice, stachyose treatment significantly reduced VRE colonization on days 9 and 10 post-infection.
34280500	2	13	theme	effort	332:337	arg1	one					320:322	one	320:322	one	320:322	Multiple measures have been investigated to address this issue, altering gut microbiota through dietary intervention represents one of such effort.
34280500	2	13	theme	effort	332:337	arg1	effort					332:337	such effort	327:337	such effort	327:337	Multiple measures have been investigated to address this issue, altering gut microbiota through dietary intervention represents one of such effort.
34280500	14	14	theme	stachyose	1998:2006	arg1	supplementation					2008:2022	stachyose supplementation	1998:2022	stachyose supplementation	1998:2022	In conclusion, stachyose supplementation can effectively inhibit VRE colonization and probably altering composition of the microbiome, which can in turn result in changes in expression of genes.
34280500	15	15	theme	expression	2261:2270	arg1	abundance					2230:2238	the abundance	2226:2238	the abundance of Lactobacillus and expression of genes involving in metabolic pathway in normal mice	2226:2325	Stachyose may also benefit health by increasing the abundance of Lactobacillus and expression of genes involving in metabolic pathway in normal mice.
34280500	12	16	theme	RNA-sequencing	1667:1680	arg1	results					1682:1688	RNA-sequencing results	1667:1688	RNA-sequencing results	1667:1688	RNA-sequencing results showed that stachyose treatment in VRE-infected mice increased expression of genes involved in TNF and IL-17 signaling pathways.
34280500	0	17	from	microbiota	82:91	arg1	mice					96:99	mice	96:99	mice	96:99	Stachyose inhibits vancomycin-resistant Enterococcus colonization and affects gut microbiota in mice.
34280500	1	18	theme	caused	142:147	arg1	infections					160:169	Vancomycin-resistant Enterococcus (VRE) caused nosocomial infections	102:169	Vancomycin-resistant Enterococcus (VRE) caused nosocomial infections	102:169	Vancomycin-resistant Enterococcus (VRE) caused nosocomial infections are rising globally.
34280500	10	19	theme	PBS-treated	1466:1476	arg1	P < 0.01					1494:1501	P < 0.01	1494:1501	P < 0.01	1494:1501	Stachyose treatment increased the relative abundance of Porphyromonadaceae, Parabacteroides, and Parabacteroides distasonis compared to the PBS-treated infection mice (P < 0.01).
34280500	10	19	theme	PBS-treated	1466:1476	arg1	mice					1488:1491	the PBS-treated infection mice	1462:1491	the PBS-treated infection mice (P < 0.01)	1462:1502	Stachyose treatment increased the relative abundance of Porphyromonadaceae, Parabacteroides, and Parabacteroides distasonis compared to the PBS-treated infection mice (P < 0.01).
34280500	2	20	theme	dietary	288:294	arg1	intervention					296:307	dietary intervention	288:307	dietary intervention represents one of such effort	288:337	Multiple measures have been investigated to address this issue, altering gut microbiota through dietary intervention represents one of such effort.
34280500	13	21	theme	metabolic	1945:1953	arg1	pathway					1955:1961	metabolic pathway	1945:1961	metabolic pathway in uninfected mice	1945:1980	Stachyose treatment also up-regulated Hsd17b14, Cyp3a44, Arg1, and down-regulated Pnliprp2, Ces1c, Pla2g4c genes involving in metabolic pathway in uninfected mice.
34280500	8	22	from	cultivation	1118:1128	arg1	experiment					1186:1195	two parallel experiment	1173:1195	two parallel experiment	1173:1195	Fresh fecal and colon samples were collected, then VRE colonization, gut microbiota and gene expression were respectively assessed using cultivation, 16s rRNA sequencing and RNA-sequencing in two parallel experiment, respectively.
34280500	12	23	theme	IL-17	1793:1797	arg1	pathways					1809:1816	IL-17 signaling pathways	1793:1816	IL-17 signaling pathways	1793:1816	RNA-sequencing results showed that stachyose treatment in VRE-infected mice increased expression of genes involved in TNF and IL-17 signaling pathways.
34280500	15	24	from	pathway	2304:2310	arg1	mice					2322:2325	normal mice	2315:2325	normal mice	2315:2325	Stachyose may also benefit health by increasing the abundance of Lactobacillus and expression of genes involving in metabolic pathway in normal mice.
34280500	5	25	theme	female	645:650	arg1	mice					660:663	6-week old female C57/6 J mice	634:663	6-week old female C57/6 J mice pre-treated with vancomycin	634:691	In VRE-infection experiment, 6-week old female C57/6 J mice pre-treated with vancomycin were infected with 2 × 108 CFU VRE via gavage.
34280500	15	26	theme	normal	2315:2320	arg1	mice					2322:2325	normal mice	2315:2325	normal mice	2315:2325	Stachyose may also benefit health by increasing the abundance of Lactobacillus and expression of genes involving in metabolic pathway in normal mice.
34280500	8	27	from	sequencing	1140:1149	arg1	experiment					1186:1195	two parallel experiment	1173:1195	two parallel experiment	1173:1195	Fresh fecal and colon samples were collected, then VRE colonization, gut microbiota and gene expression were respectively assessed using cultivation, 16s rRNA sequencing and RNA-sequencing in two parallel experiment, respectively.
34280500	14	28	from	changes	2146:2152	arg1	expression					2157:2166	expression	2157:2166	expression of genes	2157:2175	In conclusion, stachyose supplementation can effectively inhibit VRE colonization and probably altering composition of the microbiome, which can in turn result in changes in expression of genes.
34280500	4	29	theme	VRE	511:513	arg1	colonization					515:526	VRE colonization	511:526	VRE colonization	511:526	This study aimed to determine whether stachyose inhibits VRE colonization and investigated the involvement of gut microbiota this effect of stachyose.
34280500	6	30	theme	oral	765:768	arg1	administration					770:783	oral administration	765:783	oral administration of stachyose or PBS	765:803	These mice then received oral administration of stachyose or PBS as control for 7days.
34280500	8	31	theme	gene	1069:1072	arg1	expression					1074:1083	gene expression	1069:1083	gene expression	1069:1083	Fresh fecal and colon samples were collected, then VRE colonization, gut microbiota and gene expression were respectively assessed using cultivation, 16s rRNA sequencing and RNA-sequencing in two parallel experiment, respectively.
34280500	7	32	theme	normal	968:973	arg1	mice					975:978	normal mice	968:978	normal mice	968:978	Two groups of uninfected mice were also received daily gavage of stachyose or PBS for 7 days to observe the impact of stachyose treatment on normal mice.
34280500	11	33	theme	Uninfected	1505:1514	arg1	mice					1516:1519	Uninfected mice	1505:1519	Uninfected mice treated with stachyose	1505:1542	Uninfected mice treated with stachyose showed a significant increase in Lactobacillaceae and Lactobacillus compared to the PBS-treated uninfected mice(P < 0.05).
34280500	5	34	theme	6-week	634:639	arg1	mice					660:663	6-week old female C57/6 J mice	634:663	6-week old female C57/6 J mice pre-treated with vancomycin	634:691	In VRE-infection experiment, 6-week old female C57/6 J mice pre-treated with vancomycin were infected with 2 × 108 CFU VRE via gavage.
34280500	1	35	theme	Enterococcus	123:134	arg1	infections					160:169	Vancomycin-resistant Enterococcus (VRE) caused nosocomial infections	102:169	Vancomycin-resistant Enterococcus (VRE) caused nosocomial infections	102:169	Vancomycin-resistant Enterococcus (VRE) caused nosocomial infections are rising globally.
34280500	3	36	theme	good	397:400	arg1	candidate					402:410	a good candidate	395:410	a good candidate for potentially inhibiting VRE infection	395:451	Stachyose can promote probiotic growth, which makes it a good candidate for potentially inhibiting VRE infection.
34280500	4	37	theme	stachyose	594:602	arg1	effect					584:589	this effect	579:589	this effect of stachyose	579:602	This study aimed to determine whether stachyose inhibits VRE colonization and investigated the involvement of gut microbiota this effect of stachyose.
34280500	14	38	theme	microbiome	2106:2115	arg1	colonization					2052:2063	VRE colonization	2048:2063	VRE colonization	2048:2063	In conclusion, stachyose supplementation can effectively inhibit VRE colonization and probably altering composition of the microbiome, which can in turn result in changes in expression of genes.
34280500	14	38	theme	microbiome	2106:2115	arg1	composition					2087:2097	probably altering composition	2069:2097	probably altering composition	2069:2097	In conclusion, stachyose supplementation can effectively inhibit VRE colonization and probably altering composition of the microbiome, which can in turn result in changes in expression of genes.
34280500	0	39	theme	vancomycin-resistant	19:38	arg1	colonization					53:64	vancomycin-resistant Enterococcus colonization	19:64	vancomycin-resistant Enterococcus colonization	19:64	Stachyose inhibits vancomycin-resistant Enterococcus colonization and affects gut microbiota in mice.
34280500	7	40	theme	uninfected	841:850	arg1	mice					852:855	uninfected mice	841:855	uninfected mice	841:855	Two groups of uninfected mice were also received daily gavage of stachyose or PBS for 7 days to observe the impact of stachyose treatment on normal mice.
34280500	1	41	theme	VRE	137:139	arg1	infections					160:169	Vancomycin-resistant Enterococcus (VRE) caused nosocomial infections	102:169	Vancomycin-resistant Enterococcus (VRE) caused nosocomial infections	102:169	Vancomycin-resistant Enterococcus (VRE) caused nosocomial infections are rising globally.
34280500	15	42	theme	metabolic	2294:2302	arg1	pathway					2304:2310	metabolic pathway	2294:2310	metabolic pathway in normal mice	2294:2325	Stachyose may also benefit health by increasing the abundance of Lactobacillus and expression of genes involving in metabolic pathway in normal mice.
34280500	15	43	theme	Lactobacillus	2243:2255	arg1	abundance					2230:2238	the abundance	2226:2238	the abundance of Lactobacillus and expression of genes involving in metabolic pathway in normal mice	2226:2325	Stachyose may also benefit health by increasing the abundance of Lactobacillus and expression of genes involving in metabolic pathway in normal mice.
34280500	3	44	theme	inhibiting	428:437	arg1	infection					443:451	potentially inhibiting VRE infection	416:451	potentially inhibiting VRE infection	416:451	Stachyose can promote probiotic growth, which makes it a good candidate for potentially inhibiting VRE infection.
34280500	4	45	theme	microbiota	568:577	arg1	involvement					549:559	the involvement	545:559	the involvement of gut microbiota	545:577	This study aimed to determine whether stachyose inhibits VRE colonization and investigated the involvement of gut microbiota this effect of stachyose.
34280500	14	46	theme	genes	2171:2175	arg1	expression					2157:2166	expression	2157:2166	expression of genes	2157:2175	In conclusion, stachyose supplementation can effectively inhibit VRE colonization and probably altering composition of the microbiome, which can in turn result in changes in expression of genes.
34280500	2	47	theme	Multiple	192:199	arg1	measures					201:208	Multiple measures	192:208	Multiple measures	192:208	Multiple measures have been investigated to address this issue, altering gut microbiota through dietary intervention represents one of such effort.
34280500	10	48	theme	Parabacteroides	1402:1416	arg1	abundance					1369:1377	the relative abundance	1356:1377	the relative abundance of Porphyromonadaceae, Parabacteroides, and Parabacteroides distasonis	1356:1448	Stachyose treatment increased the relative abundance of Porphyromonadaceae, Parabacteroides, and Parabacteroides distasonis compared to the PBS-treated infection mice (P < 0.01).
34280500	8	49	theme	16s	1131:1133	arg1	sequencing					1140:1149	16s rRNA sequencing	1131:1149	16s rRNA sequencing	1131:1149	Fresh fecal and colon samples were collected, then VRE colonization, gut microbiota and gene expression were respectively assessed using cultivation, 16s rRNA sequencing and RNA-sequencing in two parallel experiment, respectively.
34280500	10	50	theme	Porphyromonadaceae	1382:1399	arg1	abundance					1369:1377	the relative abundance	1356:1377	the relative abundance of Porphyromonadaceae, Parabacteroides, and Parabacteroides distasonis	1356:1448	Stachyose treatment increased the relative abundance of Porphyromonadaceae, Parabacteroides, and Parabacteroides distasonis compared to the PBS-treated infection mice (P < 0.01).
34280500	7	51	theme	stachyose	945:953	arg1	treatment					955:963	stachyose treatment	945:963	stachyose treatment	945:963	Two groups of uninfected mice were also received daily gavage of stachyose or PBS for 7 days to observe the impact of stachyose treatment on normal mice.
34280500	8	52	theme	Fresh	981:985	arg1	fecal					987:991	Fresh fecal and colon samples	981:1009	fecal	987:991	Fresh fecal and colon samples were collected, then VRE colonization, gut microbiota and gene expression were respectively assessed using cultivation, 16s rRNA sequencing and RNA-sequencing in two parallel experiment, respectively.
34280500	10	53	theme	distasonis	1439:1448	arg1	abundance					1369:1377	the relative abundance	1356:1377	the relative abundance of Porphyromonadaceae, Parabacteroides, and Parabacteroides distasonis	1356:1448	Stachyose treatment increased the relative abundance of Porphyromonadaceae, Parabacteroides, and Parabacteroides distasonis compared to the PBS-treated infection mice (P < 0.01).
34280500	13	54	theme	down-regulated	1886:1899	arg1	genes					1926:1930	down-regulated Pnliprp2, Ces1c, Pla2g4c genes	1886:1930	down-regulated Pnliprp2, Ces1c, Pla2g4c genes involving in metabolic pathway in uninfected mice	1886:1980	Stachyose treatment also up-regulated Hsd17b14, Cyp3a44, Arg1, and down-regulated Pnliprp2, Ces1c, Pla2g4c genes involving in metabolic pathway in uninfected mice.
34280500	9	55	theme	VRE-infected	1215:1226	arg1	mice					1228:1231	VRE-infected mice	1215:1231	VRE-infected mice	1215:1231	In VRE-infected mice, stachyose treatment significantly reduced VRE colonization on days 9 and 10 post-infection.
34280500	2	56	theme	such	327:330	arg1	effort					332:337	such effort	327:337	such effort	327:337	Multiple measures have been investigated to address this issue, altering gut microbiota through dietary intervention represents one of such effort.
34280500	7	57	theme	treatment	955:963	arg1	impact					935:940	the impact	931:940	the impact of stachyose treatment on normal mice	931:978	Two groups of uninfected mice were also received daily gavage of stachyose or PBS for 7 days to observe the impact of stachyose treatment on normal mice.
34280500	11	58	theme	PBS-treated	1628:1638	arg1	P < 0.05					1656:1663	P < 0.05	1656:1663	P < 0.05	1656:1663	Uninfected mice treated with stachyose showed a significant increase in Lactobacillaceae and Lactobacillus compared to the PBS-treated uninfected mice(P < 0.05).
34280500	11	58	theme	PBS-treated	1628:1638	arg1	mice					1651:1654	the PBS-treated uninfected mice	1624:1654	the PBS-treated uninfected mice(P < 0.05)	1624:1664	Uninfected mice treated with stachyose showed a significant increase in Lactobacillaceae and Lactobacillus compared to the PBS-treated uninfected mice(P < 0.05).
34280500	14	59	theme	VRE	2048:2050	arg1	colonization					2052:2063	VRE colonization	2048:2063	VRE colonization	2048:2063	In conclusion, stachyose supplementation can effectively inhibit VRE colonization and probably altering composition of the microbiome, which can in turn result in changes in expression of genes.
34280500	9	60	from	colonization	1280:1291	arg1	days					1296:1299	days 9 and 10	1296:1308	days	1296:1299	In VRE-infected mice, stachyose treatment significantly reduced VRE colonization on days 9 and 10 post-infection.
34280500	9	60	from	colonization	1280:1291	arg1	10					1307:1308	10	1307:1308	10	1307:1308	In VRE-infected mice, stachyose treatment significantly reduced VRE colonization on days 9 and 10 post-infection.
34280500	12	61	theme	VRE-infected	1725:1736	arg1	mice					1738:1741	VRE-infected mice	1725:1741	VRE-infected mice	1725:1741	RNA-sequencing results showed that stachyose treatment in VRE-infected mice increased expression of genes involved in TNF and IL-17 signaling pathways.
34280500	2	62	theme	gut	265:267	arg1	microbiota					269:278	gut microbiota	265:278	gut microbiota	265:278	Multiple measures have been investigated to address this issue, altering gut microbiota through dietary intervention represents one of such effort.
34280500	7	63	theme	daily	876:880	arg1	gavage					882:887	daily gavage	876:887	daily gavage of stachyose or PBS for 7 days	876:918	Two groups of uninfected mice were also received daily gavage of stachyose or PBS for 7 days to observe the impact of stachyose treatment on normal mice.
34280500	5	64	theme	VRE-infection	608:620	arg1	experiment					622:631	VRE-infection experiment	608:631	VRE-infection experiment	608:631	In VRE-infection experiment, 6-week old female C57/6 J mice pre-treated with vancomycin were infected with 2 × 108 CFU VRE via gavage.
34280500	1	65	theme	nosocomial	149:158	arg1	infections					160:169	Vancomycin-resistant Enterococcus (VRE) caused nosocomial infections	102:169	Vancomycin-resistant Enterococcus (VRE) caused nosocomial infections	102:169	Vancomycin-resistant Enterococcus (VRE) caused nosocomial infections are rising globally.
34280500	11	66	theme	significant	1553:1563	arg1	increase					1565:1572	a significant increase	1551:1572	a significant increase in Lactobacillaceae and Lactobacillus	1551:1610	Uninfected mice treated with stachyose showed a significant increase in Lactobacillaceae and Lactobacillus compared to the PBS-treated uninfected mice(P < 0.05).
34280500	8	67	theme	gut	1050:1052	arg1	microbiota					1054:1063	gut microbiota	1050:1063	gut microbiota	1050:1063	Fresh fecal and colon samples were collected, then VRE colonization, gut microbiota and gene expression were respectively assessed using cultivation, 16s rRNA sequencing and RNA-sequencing in two parallel experiment, respectively.
34280500	3	68	theme	probiotic	362:370	arg1	growth					372:377	probiotic growth	362:377	probiotic growth	362:377	Stachyose can promote probiotic growth, which makes it a good candidate for potentially inhibiting VRE infection.
34280500	4	69	theme	gut	564:566	arg1	microbiota					568:577	gut microbiota	564:577	gut microbiota	564:577	This study aimed to determine whether stachyose inhibits VRE colonization and investigated the involvement of gut microbiota this effect of stachyose.
34280500	13	70	theme	Stachyose	1819:1827	arg1	treatment					1829:1837	Stachyose treatment	1819:1837	Stachyose treatment	1819:1837	Stachyose treatment also up-regulated Hsd17b14, Cyp3a44, Arg1, and down-regulated Pnliprp2, Ces1c, Pla2g4c genes involving in metabolic pathway in uninfected mice.
34280500	5	71	theme	C57/6 J	652:658	arg1	mice					660:663	6-week old female C57/6 J mice	634:663	6-week old female C57/6 J mice pre-treated with vancomycin	634:691	In VRE-infection experiment, 6-week old female C57/6 J mice pre-treated with vancomycin were infected with 2 × 108 CFU VRE via gavage.
34280500	12	72	theme	signaling	1799:1807	arg1	pathways					1809:1816	IL-17 signaling pathways	1793:1816	IL-17 signaling pathways	1793:1816	RNA-sequencing results showed that stachyose treatment in VRE-infected mice increased expression of genes involved in TNF and IL-17 signaling pathways.
34280500	13	73	theme	uninfected	1966:1975	arg1	mice					1977:1980	uninfected mice	1966:1980	uninfected mice	1966:1980	Stachyose treatment also up-regulated Hsd17b14, Cyp3a44, Arg1, and down-regulated Pnliprp2, Ces1c, Pla2g4c genes involving in metabolic pathway in uninfected mice.
34280500	15	74	theme	genes	2275:2279	arg1	expression					2261:2270	expression	2261:2270	expression	2261:2270	Stachyose may also benefit health by increasing the abundance of Lactobacillus and expression of genes involving in metabolic pathway in normal mice.
34280500	15	74	theme	genes	2275:2279	arg1	Lactobacillus					2243:2255	Lactobacillus	2243:2255	Lactobacillus	2243:2255	Stachyose may also benefit health by increasing the abundance of Lactobacillus and expression of genes involving in metabolic pathway in normal mice.
34280500	12	75	theme	genes	1767:1771	arg1	expression					1753:1762	expression	1753:1762	expression of genes involved in TNF and IL-17 signaling pathways	1753:1816	RNA-sequencing results showed that stachyose treatment in VRE-infected mice increased expression of genes involved in TNF and IL-17 signaling pathways.
34280500	10	76	theme	Stachyose	1326:1334	arg1	treatment					1336:1344	Stachyose treatment	1326:1344	Stachyose treatment	1326:1344	Stachyose treatment increased the relative abundance of Porphyromonadaceae, Parabacteroides, and Parabacteroides distasonis compared to the PBS-treated infection mice (P < 0.01).
34280500	1	77	theme	Vancomycin-resistant	102:121	arg1	infections					160:169	Vancomycin-resistant Enterococcus (VRE) caused nosocomial infections	102:169	Vancomycin-resistant Enterococcus (VRE) caused nosocomial infections	102:169	Vancomycin-resistant Enterococcus (VRE) caused nosocomial infections are rising globally.
34280500	7	78	from	impact	935:940	arg1	mice					975:978	normal mice	968:978	normal mice	968:978	Two groups of uninfected mice were also received daily gavage of stachyose or PBS for 7 days to observe the impact of stachyose treatment on normal mice.
34280500	0	79	theme	Enterococcus	40:51	arg1	colonization					53:64	vancomycin-resistant Enterococcus colonization	19:64	vancomycin-resistant Enterococcus colonization	19:64	Stachyose inhibits vancomycin-resistant Enterococcus colonization and affects gut microbiota in mice.
34280500	5	80	theme	old	641:643	arg1	mice					660:663	6-week old female C57/6 J mice	634:663	6-week old female C57/6 J mice pre-treated with vancomycin	634:691	In VRE-infection experiment, 6-week old female C57/6 J mice pre-treated with vancomycin were infected with 2 × 108 CFU VRE via gavage.
34280500	10	81	theme	relative	1360:1367	arg1	abundance					1369:1377	the relative abundance	1356:1377	the relative abundance of Porphyromonadaceae, Parabacteroides, and Parabacteroides distasonis	1356:1448	Stachyose treatment increased the relative abundance of Porphyromonadaceae, Parabacteroides, and Parabacteroides distasonis compared to the PBS-treated infection mice (P < 0.01).
34280500	7	82	theme	mice	852:855	arg1	groups					831:836	Two groups	827:836	Two groups of uninfected mice	827:855	Two groups of uninfected mice were also received daily gavage of stachyose or PBS for 7 days to observe the impact of stachyose treatment on normal mice.
34280500	7	82	theme	mice	852:855	arg1	mice					852:855	uninfected mice	841:855	uninfected mice	841:855	Two groups of uninfected mice were also received daily gavage of stachyose or PBS for 7 days to observe the impact of stachyose treatment on normal mice.
34280500	12	83	from	treatment	1712:1720	arg1	mice					1738:1741	VRE-infected mice	1725:1741	VRE-infected mice	1725:1741	RNA-sequencing results showed that stachyose treatment in VRE-infected mice increased expression of genes involved in TNF and IL-17 signaling pathways.
34280500	13	84	from	pathway	1955:1961	arg1	mice					1977:1980	uninfected mice	1966:1980	uninfected mice	1966:1980	Stachyose treatment also up-regulated Hsd17b14, Cyp3a44, Arg1, and down-regulated Pnliprp2, Ces1c, Pla2g4c genes involving in metabolic pathway in uninfected mice.
34280500	0	85	theme	gut	78:80	arg1	microbiota					82:91	gut microbiota	78:91	gut microbiota in mice	78:99	Stachyose inhibits vancomycin-resistant Enterococcus colonization and affects gut microbiota in mice.
34280500	8	86	theme	rRNA	1135:1138	arg1	sequencing					1140:1149	16s rRNA sequencing	1131:1149	16s rRNA sequencing	1131:1149	Fresh fecal and colon samples were collected, then VRE colonization, gut microbiota and gene expression were respectively assessed using cultivation, 16s rRNA sequencing and RNA-sequencing in two parallel experiment, respectively.
34280500	8	87	from	RNA-sequencing	1155:1168	arg1	experiment					1186:1195	two parallel experiment	1173:1195	two parallel experiment	1173:1195	Fresh fecal and colon samples were collected, then VRE colonization, gut microbiota and gene expression were respectively assessed using cultivation, 16s rRNA sequencing and RNA-sequencing in two parallel experiment, respectively.
34280500	3	88	theme	VRE	439:441	arg1	infection					443:451	potentially inhibiting VRE infection	416:451	potentially inhibiting VRE infection	416:451	Stachyose can promote probiotic growth, which makes it a good candidate for potentially inhibiting VRE infection.
34280500	14	89	theme	altering	2078:2085	arg1	composition					2087:2097	probably altering composition	2069:2097	probably altering composition	2069:2097	In conclusion, stachyose supplementation can effectively inhibit VRE colonization and probably altering composition of the microbiome, which can in turn result in changes in expression of genes.
34280500	6	90	theme	PBS	801:803	arg1	administration					770:783	oral administration	765:783	oral administration of stachyose or PBS	765:803	These mice then received oral administration of stachyose or PBS as control for 7days.
34280500	13	91	theme	Pla2g4c	1918:1924	arg1	genes					1926:1930	down-regulated Pnliprp2, Ces1c, Pla2g4c genes	1886:1930	down-regulated Pnliprp2, Ces1c, Pla2g4c genes involving in metabolic pathway in uninfected mice	1886:1980	Stachyose treatment also up-regulated Hsd17b14, Cyp3a44, Arg1, and down-regulated Pnliprp2, Ces1c, Pla2g4c genes involving in metabolic pathway in uninfected mice.
34280500	6	92	theme	stachyose	788:796	arg1	administration					770:783	oral administration	765:783	oral administration of stachyose or PBS	765:803	These mice then received oral administration of stachyose or PBS as control for 7days.
32531254	4	0	from	change	723:728	arg1	SHBG					733:736	SHBG	733:736	SHBG measured using the other monoclonal antibody pairings whereas the SHBG levels in the concordant sample declined following sialidase using the same monoclonal antibody pairings	733:912	Conversely, following sialidase treatment, the discordant sample showed no change in SHBG measured using the other monoclonal antibody pairings whereas the SHBG levels in the concordant sample declined following sialidase using the same monoclonal antibody pairings.
32531254	6	1	theme	circulating	1293:1303	arg1	antibodies					1305:1314	other circulating antibodies	1287:1314	other circulating antibodies	1287:1314	Hence rather than immunoassay discordance being attributed to heterophile antibodies or other circulating antibodies here it can be likely attributed to glycosylation affecting antibody recognition and hence the measurement of SHBG.
32531254	5	2	from	factor	950:955	arg1	recognition					969:979	antibody recognition	960:979	antibody recognition	960:979	This implicated glycosylation as a factor in antibody recognition and synthetic peptides spanning the two N-linked and one O-linked glycosylation regions showed that SHBG recognition by monoclonal antibody 7H9 could be disrupted by a peptide spanning the O-linked glycosylation site.
32531254	5	2	from	factor	950:955	arg1	peptides					995:1002	synthetic peptides	985:1002	synthetic peptides spanning the two N-linked and one O-linked glycosylation regions	985:1067	This implicated glycosylation as a factor in antibody recognition and synthetic peptides spanning the two N-linked and one O-linked glycosylation regions showed that SHBG recognition by monoclonal antibody 7H9 could be disrupted by a peptide spanning the O-linked glycosylation site.
32531254	1	3	theme	concordant	245:254	arg1	control					256:262	a concordant control	243:262	a concordant control	243:262	A discordance between sex hormone-binding globulin (SHBG) measurements by 2-site ELISAs was investigated using pairings of various "in house" SHBG antibodies together with a concordant control.
32531254	3	4	theme	concordant	629:638	arg1	sample					640:645	the concordant sample	625:645	the concordant sample	625:645	Sialidase treatment of the discordant sample and purified SHBG revealed increased levels using 7H9 whereas there was no change in SHBG in the concordant sample.
32531254	6	5	theme	other	1287:1291	arg1	antibodies					1305:1314	other circulating antibodies	1287:1314	other circulating antibodies	1287:1314	Hence rather than immunoassay discordance being attributed to heterophile antibodies or other circulating antibodies here it can be likely attributed to glycosylation affecting antibody recognition and hence the measurement of SHBG.
32531254	5	6	link	O-linked	1038:1045	arg1	regions					1061:1067	the two N-linked and one O-linked glycosylation regions	1013:1067	the two N-linked and one O-linked glycosylation regions	1013:1067	This implicated glycosylation as a factor in antibody recognition and synthetic peptides spanning the two N-linked and one O-linked glycosylation regions showed that SHBG recognition by monoclonal antibody 7H9 could be disrupted by a peptide spanning the O-linked glycosylation site.
32531254	2	7	with	paired	444:449	arg1	11F11					479:483	11F11	479:483	11F11	479:483	The 2-site monoclonal ELISAs used the same base coat (11F11) and discordance was observed with one top coat monoclonal antibody (7H9) and also when a polyclonal SHBG antibody was paired with the basecoat antibody (11F11).
32531254	2	7	with	paired	444:449	arg1	antibody					469:476	basecoat antibody	460:476	the basecoat antibody (11F11)	456:484	The 2-site monoclonal ELISAs used the same base coat (11F11) and discordance was observed with one top coat monoclonal antibody (7H9) and also when a polyclonal SHBG antibody was paired with the basecoat antibody (11F11).
32531254	2	8	used	used	294:297	arg2	ELISAs					287:292	The 2-site monoclonal ELISAs	265:292	The 2-site monoclonal ELISAs	265:292	The 2-site monoclonal ELISAs used the same base coat (11F11) and discordance was observed with one top coat monoclonal antibody (7H9) and also when a polyclonal SHBG antibody was paired with the basecoat antibody (11F11).
32531254	3	9	theme	sample	525:530	arg1	treatment					497:505	Sialidase treatment	487:505	Sialidase treatment of the discordant sample	487:530	Sialidase treatment of the discordant sample and purified SHBG revealed increased levels using 7H9 whereas there was no change in SHBG in the concordant sample.
32531254	3	9	theme	sample	525:530	arg1	SHBG					545:548	purified SHBG	536:548	purified SHBG	536:548	Sialidase treatment of the discordant sample and purified SHBG revealed increased levels using 7H9 whereas there was no change in SHBG in the concordant sample.
32531254	4	10	from	levels	809:814	arg1	sample					834:839	the concordant sample	819:839	the concordant sample	819:839	Conversely, following sialidase treatment, the discordant sample showed no change in SHBG measured using the other monoclonal antibody pairings whereas the SHBG levels in the concordant sample declined following sialidase using the same monoclonal antibody pairings.
32531254	4	11	theme	concordant	823:832	arg1	sample					834:839	the concordant sample	819:839	the concordant sample	819:839	Conversely, following sialidase treatment, the discordant sample showed no change in SHBG measured using the other monoclonal antibody pairings whereas the SHBG levels in the concordant sample declined following sialidase using the same monoclonal antibody pairings.
32531254	4	12	theme	sialidase	670:678	arg1	treatment					680:688	sialidase treatment	670:688	sialidase treatment	670:688	Conversely, following sialidase treatment, the discordant sample showed no change in SHBG measured using the other monoclonal antibody pairings whereas the SHBG levels in the concordant sample declined following sialidase using the same monoclonal antibody pairings.
32531254	5	13	theme	SHBG	1081:1084	arg1	recognition					1086:1096	SHBG recognition	1081:1096	SHBG recognition by monoclonal antibody 7H9	1081:1123	This implicated glycosylation as a factor in antibody recognition and synthetic peptides spanning the two N-linked and one O-linked glycosylation regions showed that SHBG recognition by monoclonal antibody 7H9 could be disrupted by a peptide spanning the O-linked glycosylation site.
32531254	6	14	theme	heterophile	1261:1271	arg1	antibodies					1273:1282	heterophile antibodies	1261:1282	heterophile antibodies	1261:1282	Hence rather than immunoassay discordance being attributed to heterophile antibodies or other circulating antibodies here it can be likely attributed to glycosylation affecting antibody recognition and hence the measurement of SHBG.
32531254	2	15	theme	monoclonal	276:285	arg1	ELISAs					287:292	The 2-site monoclonal ELISAs	265:292	The 2-site monoclonal ELISAs	265:292	The 2-site monoclonal ELISAs used the same base coat (11F11) and discordance was observed with one top coat monoclonal antibody (7H9) and also when a polyclonal SHBG antibody was paired with the basecoat antibody (11F11).
32531254	1	16	theme	various	194:200	arg1	antibodies					218:227	various "in house" SHBG antibodies	194:227	various "in house" SHBG antibodies together with a concordant control	194:262	A discordance between sex hormone-binding globulin (SHBG) measurements by 2-site ELISAs was investigated using pairings of various "in house" SHBG antibodies together with a concordant control.
32531254	2	17	theme	monoclonal	373:382	arg1	7H9					394:396	7H9	394:396	7H9	394:396	The 2-site monoclonal ELISAs used the same base coat (11F11) and discordance was observed with one top coat monoclonal antibody (7H9) and also when a polyclonal SHBG antibody was paired with the basecoat antibody (11F11).
32531254	2	17	theme	monoclonal	373:382	arg1	antibody					384:391	one top coat monoclonal antibody	360:391	one top coat monoclonal antibody (7H9)	360:397	The 2-site monoclonal ELISAs used the same base coat (11F11) and discordance was observed with one top coat monoclonal antibody (7H9) and also when a polyclonal SHBG antibody was paired with the basecoat antibody (11F11).
32531254	3	18	from	change	607:612	arg1	SHBG					617:620	SHBG	617:620	SHBG	617:620	Sialidase treatment of the discordant sample and purified SHBG revealed increased levels using 7H9 whereas there was no change in SHBG in the concordant sample.
32531254	3	18	from	change	607:612	arg1	sample					640:645	the concordant sample	625:645	the concordant sample	625:645	Sialidase treatment of the discordant sample and purified SHBG revealed increased levels using 7H9 whereas there was no change in SHBG in the concordant sample.
32531254	5	19	theme	glycosylation	1179:1191	arg1	site					1193:1196	the O-linked glycosylation site	1166:1196	the O-linked glycosylation site	1166:1196	This implicated glycosylation as a factor in antibody recognition and synthetic peptides spanning the two N-linked and one O-linked glycosylation regions showed that SHBG recognition by monoclonal antibody 7H9 could be disrupted by a peptide spanning the O-linked glycosylation site.
32531254	1	20	theme	sex	93:95	arg1	globulin					113:120	sex hormone-binding globulin	93:120	sex hormone-binding globulin (SHBG) measurements by 2-site ELISAs	93:157	A discordance between sex hormone-binding globulin (SHBG) measurements by 2-site ELISAs was investigated using pairings of various "in house" SHBG antibodies together with a concordant control.
32531254	1	20	theme	sex	93:95	arg1	SHBG					123:126	SHBG	123:126	SHBG	123:126	A discordance between sex hormone-binding globulin (SHBG) measurements by 2-site ELISAs was investigated using pairings of various "in house" SHBG antibodies together with a concordant control.
32531254	2	21	theme	coat	368:371	arg1	7H9					394:396	7H9	394:396	7H9	394:396	The 2-site monoclonal ELISAs used the same base coat (11F11) and discordance was observed with one top coat monoclonal antibody (7H9) and also when a polyclonal SHBG antibody was paired with the basecoat antibody (11F11).
32531254	2	21	theme	coat	368:371	arg1	antibody					384:391	one top coat monoclonal antibody	360:391	one top coat monoclonal antibody (7H9)	360:397	The 2-site monoclonal ELISAs used the same base coat (11F11) and discordance was observed with one top coat monoclonal antibody (7H9) and also when a polyclonal SHBG antibody was paired with the basecoat antibody (11F11).
32531254	5	22	gly	glycosylation	1179:1191	arg2	site					1193:1196	the O-linked glycosylation site	1166:1196	the O-linked glycosylation site	1166:1196	This implicated glycosylation as a factor in antibody recognition and synthetic peptides spanning the two N-linked and one O-linked glycosylation regions showed that SHBG recognition by monoclonal antibody 7H9 could be disrupted by a peptide spanning the O-linked glycosylation site.
32531254	1	23	theme	hormone-binding	97:111	arg1	globulin					113:120	sex hormone-binding globulin	93:120	sex hormone-binding globulin (SHBG) measurements by 2-site ELISAs	93:157	A discordance between sex hormone-binding globulin (SHBG) measurements by 2-site ELISAs was investigated using pairings of various "in house" SHBG antibodies together with a concordant control.
32531254	1	23	theme	hormone-binding	97:111	arg1	SHBG					123:126	SHBG	123:126	SHBG	123:126	A discordance between sex hormone-binding globulin (SHBG) measurements by 2-site ELISAs was investigated using pairings of various "in house" SHBG antibodies together with a concordant control.
32531254	2	24	theme	top	364:366	arg1	7H9					394:396	7H9	394:396	7H9	394:396	The 2-site monoclonal ELISAs used the same base coat (11F11) and discordance was observed with one top coat monoclonal antibody (7H9) and also when a polyclonal SHBG antibody was paired with the basecoat antibody (11F11).
32531254	2	24	theme	top	364:366	arg1	antibody					384:391	one top coat monoclonal antibody	360:391	one top coat monoclonal antibody (7H9)	360:397	The 2-site monoclonal ELISAs used the same base coat (11F11) and discordance was observed with one top coat monoclonal antibody (7H9) and also when a polyclonal SHBG antibody was paired with the basecoat antibody (11F11).
32531254	4	25	theme	SHBG	804:807	arg1	levels					809:814	the SHBG levels	800:814	the SHBG levels in the concordant sample	800:839	Conversely, following sialidase treatment, the discordant sample showed no change in SHBG measured using the other monoclonal antibody pairings whereas the SHBG levels in the concordant sample declined following sialidase using the same monoclonal antibody pairings.
32531254	1	26	theme	globulin	113:120	arg1	measurements					129:140	sex hormone-binding globulin (SHBG) measurements	93:140	sex hormone-binding globulin (SHBG) measurements by 2-site ELISAs	93:157	A discordance between sex hormone-binding globulin (SHBG) measurements by 2-site ELISAs was investigated using pairings of various "in house" SHBG antibodies together with a concordant control.
32531254	5	27	theme	glycosylation	1047:1059	arg1	regions					1061:1067	the two N-linked and one O-linked glycosylation regions	1013:1067	the two N-linked and one O-linked glycosylation regions	1013:1067	This implicated glycosylation as a factor in antibody recognition and synthetic peptides spanning the two N-linked and one O-linked glycosylation regions showed that SHBG recognition by monoclonal antibody 7H9 could be disrupted by a peptide spanning the O-linked glycosylation site.
32531254	0	28	theme	sex	28:30	arg1	globulin					48:55	sex hormone-binding globulin	28:55	sex hormone-binding globulin measurements	28:68	Glycosylation may influence sex hormone-binding globulin measurements.
32531254	4	29	theme	same	880:883	arg1	pairings					905:912	the same monoclonal antibody pairings	876:912	the same monoclonal antibody pairings	876:912	Conversely, following sialidase treatment, the discordant sample showed no change in SHBG measured using the other monoclonal antibody pairings whereas the SHBG levels in the concordant sample declined following sialidase using the same monoclonal antibody pairings.
32531254	2	30	theme	same	303:306	arg1	11F11					319:323	11F11	319:323	11F11	319:323	The 2-site monoclonal ELISAs used the same base coat (11F11) and discordance was observed with one top coat monoclonal antibody (7H9) and also when a polyclonal SHBG antibody was paired with the basecoat antibody (11F11).
32531254	2	30	theme	same	303:306	arg1	coat					313:316	the same base coat	299:316	the same base coat (11F11)	299:324	The 2-site monoclonal ELISAs used the same base coat (11F11) and discordance was observed with one top coat monoclonal antibody (7H9) and also when a polyclonal SHBG antibody was paired with the basecoat antibody (11F11).
32531254	5	31	theme	synthetic	985:993	arg1	peptides					995:1002	synthetic peptides	985:1002	synthetic peptides spanning the two N-linked and one O-linked glycosylation regions	985:1067	This implicated glycosylation as a factor in antibody recognition and synthetic peptides spanning the two N-linked and one O-linked glycosylation regions showed that SHBG recognition by monoclonal antibody 7H9 could be disrupted by a peptide spanning the O-linked glycosylation site.
32531254	1	32	theme	in	203:204	arg1	antibodies					218:227	various "in house" SHBG antibodies	194:227	various "in house" SHBG antibodies together with a concordant control	194:262	A discordance between sex hormone-binding globulin (SHBG) measurements by 2-site ELISAs was investigated using pairings of various "in house" SHBG antibodies together with a concordant control.
32531254	2	33	theme	base	308:311	arg1	11F11					319:323	11F11	319:323	11F11	319:323	The 2-site monoclonal ELISAs used the same base coat (11F11) and discordance was observed with one top coat monoclonal antibody (7H9) and also when a polyclonal SHBG antibody was paired with the basecoat antibody (11F11).
32531254	2	33	theme	base	308:311	arg1	coat					313:316	the same base coat	299:316	the same base coat (11F11)	299:324	The 2-site monoclonal ELISAs used the same base coat (11F11) and discordance was observed with one top coat monoclonal antibody (7H9) and also when a polyclonal SHBG antibody was paired with the basecoat antibody (11F11).
32531254	5	34	theme	O-linked	1038:1045	arg1	regions					1061:1067	the two N-linked and one O-linked glycosylation regions	1013:1067	the two N-linked and one O-linked glycosylation regions	1013:1067	This implicated glycosylation as a factor in antibody recognition and synthetic peptides spanning the two N-linked and one O-linked glycosylation regions showed that SHBG recognition by monoclonal antibody 7H9 could be disrupted by a peptide spanning the O-linked glycosylation site.
32531254	3	35	theme	discordant	514:523	arg1	sample					525:530	the discordant sample	510:530	the discordant sample	510:530	Sialidase treatment of the discordant sample and purified SHBG revealed increased levels using 7H9 whereas there was no change in SHBG in the concordant sample.
32531254	1	36	theme	house	206:210	arg1	antibodies					218:227	various "in house" SHBG antibodies	194:227	various "in house" SHBG antibodies together with a concordant control	194:262	A discordance between sex hormone-binding globulin (SHBG) measurements by 2-site ELISAs was investigated using pairings of various "in house" SHBG antibodies together with a concordant control.
32531254	0	37	theme	globulin	48:55	arg1	measurements					57:68	sex hormone-binding globulin measurements	28:68	sex hormone-binding globulin measurements	28:68	Glycosylation may influence sex hormone-binding globulin measurements.
32531254	5	38	theme	O-linked	1170:1177	arg1	site					1193:1196	the O-linked glycosylation site	1166:1196	the O-linked glycosylation site	1166:1196	This implicated glycosylation as a factor in antibody recognition and synthetic peptides spanning the two N-linked and one O-linked glycosylation regions showed that SHBG recognition by monoclonal antibody 7H9 could be disrupted by a peptide spanning the O-linked glycosylation site.
32531254	4	39	theme	antibody	896:903	arg1	pairings					905:912	the same monoclonal antibody pairings	876:912	the same monoclonal antibody pairings	876:912	Conversely, following sialidase treatment, the discordant sample showed no change in SHBG measured using the other monoclonal antibody pairings whereas the SHBG levels in the concordant sample declined following sialidase using the same monoclonal antibody pairings.
32531254	3	40	theme	Sialidase	487:495	arg1	treatment					497:505	Sialidase treatment	487:505	Sialidase treatment of the discordant sample	487:530	Sialidase treatment of the discordant sample and purified SHBG revealed increased levels using 7H9 whereas there was no change in SHBG in the concordant sample.
32531254	4	41	theme	monoclonal	763:772	arg1	pairings					783:790	the other monoclonal antibody pairings	753:790	the other monoclonal antibody pairings whereas the SHBG levels in the concordant sample declined following sialidase using the same monoclonal antibody pairings	753:912	Conversely, following sialidase treatment, the discordant sample showed no change in SHBG measured using the other monoclonal antibody pairings whereas the SHBG levels in the concordant sample declined following sialidase using the same monoclonal antibody pairings.
32531254	1	42	theme	"	211:211	arg1	antibodies					218:227	various "in house" SHBG antibodies	194:227	various "in house" SHBG antibodies together with a concordant control	194:262	A discordance between sex hormone-binding globulin (SHBG) measurements by 2-site ELISAs was investigated using pairings of various "in house" SHBG antibodies together with a concordant control.
32531254	0	43	theme	hormone-binding	32:46	arg1	globulin					48:55	sex hormone-binding globulin	28:55	sex hormone-binding globulin measurements	28:68	Glycosylation may influence sex hormone-binding globulin measurements.
32531254	4	44	theme	monoclonal	885:894	arg1	pairings					905:912	the same monoclonal antibody pairings	876:912	the same monoclonal antibody pairings	876:912	Conversely, following sialidase treatment, the discordant sample showed no change in SHBG measured using the other monoclonal antibody pairings whereas the SHBG levels in the concordant sample declined following sialidase using the same monoclonal antibody pairings.
32531254	3	45	theme	increased	559:567	arg1	levels					569:574	increased levels	559:574	increased levels using 7H9	559:584	Sialidase treatment of the discordant sample and purified SHBG revealed increased levels using 7H9 whereas there was no change in SHBG in the concordant sample.
32531254	1	46	theme	SHBG	213:216	arg1	antibodies					218:227	various "in house" SHBG antibodies	194:227	various "in house" SHBG antibodies together with a concordant control	194:262	A discordance between sex hormone-binding globulin (SHBG) measurements by 2-site ELISAs was investigated using pairings of various "in house" SHBG antibodies together with a concordant control.
32531254	2	47	theme	SHBG	426:429	arg1	paired					444:449	paired	444:449	paired	444:449	The 2-site monoclonal ELISAs used the same base coat (11F11) and discordance was observed with one top coat monoclonal antibody (7H9) and also when a polyclonal SHBG antibody was paired with the basecoat antibody (11F11).
32531254	2	47	theme	SHBG	426:429	arg1	antibody					431:438	a polyclonal SHBG antibody	413:438	a polyclonal SHBG antibody	413:438	The 2-site monoclonal ELISAs used the same base coat (11F11) and discordance was observed with one top coat monoclonal antibody (7H9) and also when a polyclonal SHBG antibody was paired with the basecoat antibody (11F11).
32531254	3	48	theme	purified	536:543	arg1	SHBG					545:548	purified SHBG	536:548	purified SHBG	536:548	Sialidase treatment of the discordant sample and purified SHBG revealed increased levels using 7H9 whereas there was no change in SHBG in the concordant sample.
32531254	2	49	theme	polyclonal	415:424	arg1	paired					444:449	paired	444:449	paired	444:449	The 2-site monoclonal ELISAs used the same base coat (11F11) and discordance was observed with one top coat monoclonal antibody (7H9) and also when a polyclonal SHBG antibody was paired with the basecoat antibody (11F11).
32531254	2	49	theme	polyclonal	415:424	arg1	antibody					431:438	a polyclonal SHBG antibody	413:438	a polyclonal SHBG antibody	413:438	The 2-site monoclonal ELISAs used the same base coat (11F11) and discordance was observed with one top coat monoclonal antibody (7H9) and also when a polyclonal SHBG antibody was paired with the basecoat antibody (11F11).
32531254	6	50	theme	immunoassay	1217:1227	arg1	discordance					1229:1239	immunoassay discordance	1217:1239	immunoassay discordance	1217:1239	Hence rather than immunoassay discordance being attributed to heterophile antibodies or other circulating antibodies here it can be likely attributed to glycosylation affecting antibody recognition and hence the measurement of SHBG.
32531254	4	51	theme	antibody	774:781	arg1	pairings					783:790	the other monoclonal antibody pairings	753:790	the other monoclonal antibody pairings whereas the SHBG levels in the concordant sample declined following sialidase using the same monoclonal antibody pairings	753:912	Conversely, following sialidase treatment, the discordant sample showed no change in SHBG measured using the other monoclonal antibody pairings whereas the SHBG levels in the concordant sample declined following sialidase using the same monoclonal antibody pairings.
32531254	5	52	theme	N-linked	1021:1028	arg1	regions					1061:1067	the two N-linked and one O-linked glycosylation regions	1013:1067	the two N-linked and one O-linked glycosylation regions	1013:1067	This implicated glycosylation as a factor in antibody recognition and synthetic peptides spanning the two N-linked and one O-linked glycosylation regions showed that SHBG recognition by monoclonal antibody 7H9 could be disrupted by a peptide spanning the O-linked glycosylation site.
32531254	4	53	theme	other	757:761	arg1	pairings					783:790	the other monoclonal antibody pairings	753:790	the other monoclonal antibody pairings whereas the SHBG levels in the concordant sample declined following sialidase using the same monoclonal antibody pairings	753:912	Conversely, following sialidase treatment, the discordant sample showed no change in SHBG measured using the other monoclonal antibody pairings whereas the SHBG levels in the concordant sample declined following sialidase using the same monoclonal antibody pairings.
32531254	1	54	theme	antibodies	218:227	arg1	pairings					182:189	pairings	182:189	pairings of various "in house" SHBG antibodies together with a concordant control	182:262	A discordance between sex hormone-binding globulin (SHBG) measurements by 2-site ELISAs was investigated using pairings of various "in house" SHBG antibodies together with a concordant control.
32531254	2	55	theme	2-site	269:274	arg1	ELISAs					287:292	The 2-site monoclonal ELISAs	265:292	The 2-site monoclonal ELISAs	265:292	The 2-site monoclonal ELISAs used the same base coat (11F11) and discordance was observed with one top coat monoclonal antibody (7H9) and also when a polyclonal SHBG antibody was paired with the basecoat antibody (11F11).
32531254	5	56	theme	antibody	960:967	arg1	recognition					969:979	antibody recognition	960:979	antibody recognition	960:979	This implicated glycosylation as a factor in antibody recognition and synthetic peptides spanning the two N-linked and one O-linked glycosylation regions showed that SHBG recognition by monoclonal antibody 7H9 could be disrupted by a peptide spanning the O-linked glycosylation site.
32531254	4	57	theme	discordant	695:704	arg1	sample					706:711	the discordant sample	691:711	the discordant sample	691:711	Conversely, following sialidase treatment, the discordant sample showed no change in SHBG measured using the other monoclonal antibody pairings whereas the SHBG levels in the concordant sample declined following sialidase using the same monoclonal antibody pairings.
32531254	1	58	theme	2-site	145:150	arg1	ELISAs					152:157	2-site ELISAs	145:157	2-site ELISAs	145:157	A discordance between sex hormone-binding globulin (SHBG) measurements by 2-site ELISAs was investigated using pairings of various "in house" SHBG antibodies together with a concordant control.
32531254	2	59	theme	basecoat	460:467	arg1	11F11					479:483	11F11	479:483	11F11	479:483	The 2-site monoclonal ELISAs used the same base coat (11F11) and discordance was observed with one top coat monoclonal antibody (7H9) and also when a polyclonal SHBG antibody was paired with the basecoat antibody (11F11).
32531254	2	59	theme	basecoat	460:467	arg1	antibody					469:476	basecoat antibody	460:476	the basecoat antibody (11F11)	456:484	The 2-site monoclonal ELISAs used the same base coat (11F11) and discordance was observed with one top coat monoclonal antibody (7H9) and also when a polyclonal SHBG antibody was paired with the basecoat antibody (11F11).
32531254	5	60	link	O-linked	1170:1177	arg1	site					1193:1196	the O-linked glycosylation site	1166:1196	the O-linked glycosylation site	1166:1196	This implicated glycosylation as a factor in antibody recognition and synthetic peptides spanning the two N-linked and one O-linked glycosylation regions showed that SHBG recognition by monoclonal antibody 7H9 could be disrupted by a peptide spanning the O-linked glycosylation site.
32531254	5	61	theme	implicated	920:929	arg1	glycosylation					931:943	This implicated glycosylation	915:943	This implicated glycosylation as a factor in antibody recognition and synthetic peptides spanning the two N-linked and one O-linked glycosylation regions	915:1067	This implicated glycosylation as a factor in antibody recognition and synthetic peptides spanning the two N-linked and one O-linked glycosylation regions showed that SHBG recognition by monoclonal antibody 7H9 could be disrupted by a peptide spanning the O-linked glycosylation site.
32531254	5	62	link	N-linked	1021:1028	arg1	regions					1061:1067	the two N-linked and one O-linked glycosylation regions	1013:1067	the two N-linked and one O-linked glycosylation regions	1013:1067	This implicated glycosylation as a factor in antibody recognition and synthetic peptides spanning the two N-linked and one O-linked glycosylation regions showed that SHBG recognition by monoclonal antibody 7H9 could be disrupted by a peptide spanning the O-linked glycosylation site.
32531254	6	63	theme	SHBG	1426:1429	arg1	recognition					1385:1395	antibody recognition	1376:1395	antibody recognition	1376:1395	Hence rather than immunoassay discordance being attributed to heterophile antibodies or other circulating antibodies here it can be likely attributed to glycosylation affecting antibody recognition and hence the measurement of SHBG.
32531254	6	63	theme	SHBG	1426:1429	arg1	measurement					1411:1421	the measurement	1407:1421	the measurement of SHBG	1407:1429	Hence rather than immunoassay discordance being attributed to heterophile antibodies or other circulating antibodies here it can be likely attributed to glycosylation affecting antibody recognition and hence the measurement of SHBG.
32531254	5	64	theme	monoclonal	1101:1110	arg1	antibody					1112:1119	monoclonal antibody 7H9	1101:1123	monoclonal antibody 7H9	1101:1123	This implicated glycosylation as a factor in antibody recognition and synthetic peptides spanning the two N-linked and one O-linked glycosylation regions showed that SHBG recognition by monoclonal antibody 7H9 could be disrupted by a peptide spanning the O-linked glycosylation site.
32531254	6	65	theme	antibody	1376:1383	arg1	recognition					1385:1395	antibody recognition	1376:1395	antibody recognition	1376:1395	Hence rather than immunoassay discordance being attributed to heterophile antibodies or other circulating antibodies here it can be likely attributed to glycosylation affecting antibody recognition and hence the measurement of SHBG.
33864544	6	0	theme	fatty	883:887	arg1	acids					889:893	The major fatty acids	873:893	The major fatty acids of strain SM1970T	873:911	The major fatty acids of strain SM1970T were summed feature 3 (C16: 1 ω7c and/or C16: 1 ω6c), C16: 0 and summed feature 8 (C18: 1 ω7c and/or C18: 1 ω6c).
33864544	7	1	theme	respiratory	1136:1146	arg1	quinone					1148:1154	its main respiratory quinone	1127:1154	its main respiratory quinone	1127:1154	The major polar lipids of the strain included phosphatidylethanolamine and phosphatidylglycerol and its main respiratory quinone was ubiquinone 8.
33864544	5	2	theme	other	819:823	arg1	species					831:837	other known species	819:837	other known species in the class Gammaproteobacteria	819:870	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain SM1970T formed a distinct lineage close to the genus Catenovulum within the family Alteromonadaceae, sharing the highest sequence similarity (93.6%) with type strain of Catenovulum maritimum but < 93.0% sequence similarity with those of other known species in the class Gammaproteobacteria.
33864544	2	3	theme	seawater	306:313	arg1	sample					315:320	a seawater sample	304:320	a seawater sample collected from the Mariana Trench	304:354	A novel Gram-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative bacterium, designated strain SM1970T, was isolated from a seawater sample collected from the Mariana Trench.
33864544	9	4	theme	acid	1344:1347	arg1	AAI					1359:1361	AAI	1359:1361	AAI	1359:1361	The average amino acid identity (AAI) values between SM1970T and type strains of known Catenovulum species were 56.6-57.1% while the percentage of conserved proteins (POCP) values between them were 28.5-31.5%.
33864544	9	4	theme	acid	1344:1347	arg1	identity					1349:1356	average amino acid identity	1330:1356	The average amino acid identity (AAI) values between SM1970T and type strains of known Catenovulum species	1326:1431	The average amino acid identity (AAI) values between SM1970T and type strains of known Catenovulum species were 56.6-57.1% while the percentage of conserved proteins (POCP) values between them were 28.5-31.5%.
33864544	11	5	theme	strain	1640:1645	arg1	SM1970T					1647:1653	strain SM1970T	1640:1653	strain SM1970T	1640:1653	On the basis of the polyphasic analysis, strain SM1970T is considered to represent a novel species in a novel genus of the family Alteromonadaceae, for which the name Marinifaba aquimaris is proposed with the type strain being SM1970T (= MCCC 1K04323T = KCTC 72844T).
33864544	5	6	with	similarity	712:721	arg1	maritimum					763:771	type strain of Catenovulum maritimum	736:771	type strain of Catenovulum maritimum	736:771	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain SM1970T formed a distinct lineage close to the genus Catenovulum within the family Alteromonadaceae, sharing the highest sequence similarity (93.6%) with type strain of Catenovulum maritimum but < 93.0% sequence similarity with those of other known species in the class Gammaproteobacteria.
33864544	8	7	theme	draft	1178:1182	arg1	4,172,146 bp					1243:1254	4,172,146 bp	1243:1254	4,172,146 bp	1243:1254	The draft genome of strain SM1970T consisted of 77 scaffolds and was 4,172,146 bp in length, containing a complete set of genes for chitin degradation.
33864544	8	7	theme	draft	1178:1182	arg1	genome					1184:1189	The draft genome	1174:1189	The draft genome of strain SM1970T	1174:1207	The draft genome of strain SM1970T consisted of 77 scaffolds and was 4,172,146 bp in length, containing a complete set of genes for chitin degradation.
33864544	2	8	attach	isolated	290:297	arg2	bacterium					248:256	A novel Gram-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative bacterium	165:256	A novel Gram-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative bacterium	165:256	A novel Gram-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative bacterium, designated strain SM1970T, was isolated from a seawater sample collected from the Mariana Trench.
33864544	2	8	attach	isolated	290:297	arg1	sample					315:320	a seawater sample	304:320	a seawater sample collected from the Mariana Trench	304:354	A novel Gram-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative bacterium, designated strain SM1970T, was isolated from a seawater sample collected from the Mariana Trench.
33864544	8	9	theme	SM1970T	1201:1207	arg1	4,172,146 bp					1243:1254	4,172,146 bp	1243:1254	4,172,146 bp	1243:1254	The draft genome of strain SM1970T consisted of 77 scaffolds and was 4,172,146 bp in length, containing a complete set of genes for chitin degradation.
33864544	8	9	theme	SM1970T	1201:1207	arg1	genome					1184:1189	The draft genome	1174:1189	The draft genome of strain SM1970T	1174:1207	The draft genome of strain SM1970T consisted of 77 scaffolds and was 4,172,146 bp in length, containing a complete set of genes for chitin degradation.
33864544	11	10	dep	analysis	1630:1637	arg1	basis					1606:1610	basis	1606:1610	basis	1606:1610	On the basis of the polyphasic analysis, strain SM1970T is considered to represent a novel species in a novel genus of the family Alteromonadaceae, for which the name Marinifaba aquimaris is proposed with the type strain being SM1970T (= MCCC 1K04323T = KCTC 72844T).
33864544	11	10	dep	analysis	1630:1637	arg1	the					1602:1604	the	1602:1604	the	1602:1604	On the basis of the polyphasic analysis, strain SM1970T is considered to represent a novel species in a novel genus of the family Alteromonadaceae, for which the name Marinifaba aquimaris is proposed with the type strain being SM1970T (= MCCC 1K04323T = KCTC 72844T).
33864544	11	11	theme	= MCCC	1835:1840	arg1	SM1970T					1826:1832	SM1970T	1826:1832	SM1970T (= MCCC 1K04323T = KCTC 72844T)	1826:1864	On the basis of the polyphasic analysis, strain SM1970T is considered to represent a novel species in a novel genus of the family Alteromonadaceae, for which the name Marinifaba aquimaris is proposed with the type strain being SM1970T (= MCCC 1K04323T = KCTC 72844T).
33864544	11	11	theme	= MCCC	1835:1840	arg1	72844T					1858:1863	= MCCC 1K04323T = KCTC 72844T	1835:1863	= MCCC 1K04323T = KCTC 72844T	1835:1863	On the basis of the polyphasic analysis, strain SM1970T is considered to represent a novel species in a novel genus of the family Alteromonadaceae, for which the name Marinifaba aquimaris is proposed with the type strain being SM1970T (= MCCC 1K04323T = KCTC 72844T).
33864544	8	12	contain	containing	1267:1276	arg2	genes					1296:1300	genes	1296:1300	genes for chitin degradation	1296:1323	The draft genome of strain SM1970T consisted of 77 scaffolds and was 4,172,146 bp in length, containing a complete set of genes for chitin degradation.
33864544	8	12	contain	containing	1267:1276	arg2	set					1289:1291	a complete set	1278:1291	a complete set of genes for chitin degradation	1278:1323	The draft genome of strain SM1970T consisted of 77 scaffolds and was 4,172,146 bp in length, containing a complete set of genes for chitin degradation.
33864544	8	12	contain	containing	1267:1276	arg1	length					1259:1264	length	1259:1264	length	1259:1264	The draft genome of strain SM1970T consisted of 77 scaffolds and was 4,172,146 bp in length, containing a complete set of genes for chitin degradation.
33864544	7	13	theme	main	1131:1134	arg1	quinone					1148:1154	its main respiratory quinone	1127:1154	its main respiratory quinone	1127:1154	The major polar lipids of the strain included phosphatidylethanolamine and phosphatidylglycerol and its main respiratory quinone was ubiquinone 8.
33864544	6	14	dep	C16	936:938	arg1	 1 ω7c					940:945	 1 ω7c	940:945	 1 ω7c	940:945	The major fatty acids of strain SM1970T were summed feature 3 (C16: 1 ω7c and/or C16: 1 ω6c), C16: 0 and summed feature 8 (C18: 1 ω7c and/or C18: 1 ω6c).
33864544	6	14	dep	C16	936:938	arg1	 1 ω6c					958:963	 1 ω6c	958:963	 1 ω6c	958:963	The major fatty acids of strain SM1970T were summed feature 3 (C16: 1 ω7c and/or C16: 1 ω6c), C16: 0 and summed feature 8 (C18: 1 ω7c and/or C18: 1 ω6c).
33864544	6	14	dep	C16	936:938	arg1	C16					954:956	C16	954:956	C16	954:956	The major fatty acids of strain SM1970T were summed feature 3 (C16: 1 ω7c and/or C16: 1 ω6c), C16: 0 and summed feature 8 (C18: 1 ω7c and/or C18: 1 ω6c).
33864544	5	15	theme	gene	546:549	arg1	sequences					551:559	the 16S rRNA gene sequences	533:559	the 16S rRNA gene sequences	533:559	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain SM1970T formed a distinct lineage close to the genus Catenovulum within the family Alteromonadaceae, sharing the highest sequence similarity (93.6%) with type strain of Catenovulum maritimum but < 93.0% sequence similarity with those of other known species in the class Gammaproteobacteria.
33864544	1	16	theme	Trench	157:162	arg1	seawater					133:140	seawater	133:140	seawater of the Mariana Trench	133:162	nov., a novel chitin-degrading gammaproteobacterium in the family Alteromonadaceae isolated from seawater of the Mariana Trench.
33864544	5	17	theme	sequence	703:710	arg1	similarity					712:721	the highest sequence similarity	691:721	the highest sequence similarity (93.6%) with type strain of Catenovulum maritimum	691:771	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain SM1970T formed a distinct lineage close to the genus Catenovulum within the family Alteromonadaceae, sharing the highest sequence similarity (93.6%) with type strain of Catenovulum maritimum but < 93.0% sequence similarity with those of other known species in the class Gammaproteobacteria.
33864544	5	17	theme	sequence	703:710	arg1	%					728:728	93.6%	724:728	93.6%	724:728	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain SM1970T formed a distinct lineage close to the genus Catenovulum within the family Alteromonadaceae, sharing the highest sequence similarity (93.6%) with type strain of Catenovulum maritimum but < 93.0% sequence similarity with those of other known species in the class Gammaproteobacteria.
33864544	1	18	theme	chitin-degrading	50:65	arg1	gammaproteobacterium					67:86	a novel chitin-degrading gammaproteobacterium	42:86	a novel chitin-degrading gammaproteobacterium in the family Alteromonadaceae isolated from seawater of the Mariana Trench	42:162	nov., a novel chitin-degrading gammaproteobacterium in the family Alteromonadaceae isolated from seawater of the Mariana Trench.
33864544	1	18	theme	chitin-degrading	50:65	arg1	nov.					36:39	nov.	36:39	nov.	36:39	nov., a novel chitin-degrading gammaproteobacterium in the family Alteromonadaceae isolated from seawater of the Mariana Trench.
33864544	0	19	theme	Marinifaba	0:9	arg1	nov.					26:29	Marinifaba aquimaris gen. nov.	0:29	Marinifaba aquimaris gen. nov.	0:29	Marinifaba aquimaris gen. nov., sp.
33864544	5	20	theme	16S	537:539	arg1	sequences					551:559	the 16S rRNA gene sequences	533:559	the 16S rRNA gene sequences	533:559	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain SM1970T formed a distinct lineage close to the genus Catenovulum within the family Alteromonadaceae, sharing the highest sequence similarity (93.6%) with type strain of Catenovulum maritimum but < 93.0% sequence similarity with those of other known species in the class Gammaproteobacteria.
33864544	10	21	theme	G + C	1552:1556	arg1	content					1558:1564	The genomic DNA G + C content	1536:1564	The genomic DNA G + C content of strain SM1970T	1536:1582	The genomic DNA G + C content of strain SM1970T was 40.1 mol%.
33864544	10	21	theme	G + C	1552:1556	arg1	%					1596:1596	40.1 mol%	1588:1596	40.1 mol%	1588:1596	The genomic DNA G + C content of strain SM1970T was 40.1 mol%.
33864544	0	22	theme	gen.	21:24	arg1	nov.					26:29	Marinifaba aquimaris gen. nov.	0:29	Marinifaba aquimaris gen. nov.	0:29	Marinifaba aquimaris gen. nov., sp.
33864544	1	23	theme	family	95:100	arg1	Alteromonadaceae					102:117	the family Alteromonadaceae	91:117	the family Alteromonadaceae isolated from seawater of the Mariana Trench	91:162	nov., a novel chitin-degrading gammaproteobacterium in the family Alteromonadaceae isolated from seawater of the Mariana Trench.
33864544	3	24	theme	1-5	398:400	arg1	%					401:401	%	401:401	%	401:401	Strain SM1970T grew at 15-37 oC and with 1-5% (w/v) NaCl.
33864544	2	25	theme	novel	167:171	arg1	bacterium					248:256	A novel Gram-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative bacterium	165:256	A novel Gram-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative bacterium	165:256	A novel Gram-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative bacterium, designated strain SM1970T, was isolated from a seawater sample collected from the Mariana Trench.
33864544	6	26	dep	C18	996:998	arg1	C18					1014:1016	C18	1014:1016	C18	1014:1016	The major fatty acids of strain SM1970T were summed feature 3 (C16: 1 ω7c and/or C16: 1 ω6c), C16: 0 and summed feature 8 (C18: 1 ω7c and/or C18: 1 ω6c).
33864544	6	26	dep	C18	996:998	arg1	 1 ω7c					1000:1005	 1 ω7c	1000:1005	 1 ω7c	1000:1005	The major fatty acids of strain SM1970T were summed feature 3 (C16: 1 ω7c and/or C16: 1 ω6c), C16: 0 and summed feature 8 (C18: 1 ω7c and/or C18: 1 ω6c).
33864544	6	26	dep	C18	996:998	arg1	 1 ω6c					1018:1023	 1 ω6c	1018:1023	C18: 1 ω7c and/or C18: 1 ω6c	996:1023	The major fatty acids of strain SM1970T were summed feature 3 (C16: 1 ω7c and/or C16: 1 ω6c), C16: 0 and summed feature 8 (C18: 1 ω7c and/or C18: 1 ω6c).
33864544	11	27	theme	name Marinifaba	1761:1775	arg1	aquimaris					1777:1785	the name Marinifaba aquimaris	1757:1785	the name Marinifaba aquimaris	1757:1785	On the basis of the polyphasic analysis, strain SM1970T is considered to represent a novel species in a novel genus of the family Alteromonadaceae, for which the name Marinifaba aquimaris is proposed with the type strain being SM1970T (= MCCC 1K04323T = KCTC 72844T).
33864544	7	28	theme	major	1031:1035	arg1	lipids					1043:1048	The major polar lipids	1027:1048	The major polar lipids of the strain	1027:1062	The major polar lipids of the strain included phosphatidylethanolamine and phosphatidylglycerol and its main respiratory quinone was ubiquinone 8.
33864544	5	29	theme	strain	741:746	arg1	maritimum					763:771	type strain of Catenovulum maritimum	736:771	type strain of Catenovulum maritimum	736:771	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain SM1970T formed a distinct lineage close to the genus Catenovulum within the family Alteromonadaceae, sharing the highest sequence similarity (93.6%) with type strain of Catenovulum maritimum but < 93.0% sequence similarity with those of other known species in the class Gammaproteobacteria.
33864544	9	30	theme	proteins	1483:1490	arg1	percentage					1459:1468	the percentage	1455:1468	the percentage of conserved proteins (POCP) values between them	1455:1517	The average amino acid identity (AAI) values between SM1970T and type strains of known Catenovulum species were 56.6-57.1% while the percentage of conserved proteins (POCP) values between them were 28.5-31.5%.
33864544	8	31	theme	genes	1296:1300	arg1	genes					1296:1300	genes	1296:1300	genes for chitin degradation	1296:1323	The draft genome of strain SM1970T consisted of 77 scaffolds and was 4,172,146 bp in length, containing a complete set of genes for chitin degradation.
33864544	8	31	theme	genes	1296:1300	arg1	set					1289:1291	a complete set	1278:1291	a complete set of genes for chitin degradation	1278:1323	The draft genome of strain SM1970T consisted of 77 scaffolds and was 4,172,146 bp in length, containing a complete set of genes for chitin degradation.
33864544	10	32	theme	SM1970T	1576:1582	arg1	content					1558:1564	The genomic DNA G + C content	1536:1564	The genomic DNA G + C content of strain SM1970T	1536:1582	The genomic DNA G + C content of strain SM1970T was 40.1 mol%.
33864544	10	32	theme	SM1970T	1576:1582	arg1	%					1596:1596	40.1 mol%	1588:1596	40.1 mol%	1588:1596	The genomic DNA G + C content of strain SM1970T was 40.1 mol%.
33864544	8	33	theme	chitin	1306:1311	arg1	degradation					1313:1323	chitin degradation	1306:1323	chitin degradation	1306:1323	The draft genome of strain SM1970T consisted of 77 scaffolds and was 4,172,146 bp in length, containing a complete set of genes for chitin degradation.
33864544	8	34	from	4,172,146 bp	1243:1254	arg1	length					1259:1264	length	1259:1264	length	1259:1264	The draft genome of strain SM1970T consisted of 77 scaffolds and was 4,172,146 bp in length, containing a complete set of genes for chitin degradation.
33864544	9	35	theme	average	1330:1336	arg1	AAI					1359:1361	AAI	1359:1361	AAI	1359:1361	The average amino acid identity (AAI) values between SM1970T and type strains of known Catenovulum species were 56.6-57.1% while the percentage of conserved proteins (POCP) values between them were 28.5-31.5%.
33864544	9	35	theme	average	1330:1336	arg1	identity					1349:1356	average amino acid identity	1330:1356	The average amino acid identity (AAI) values between SM1970T and type strains of known Catenovulum species	1326:1431	The average amino acid identity (AAI) values between SM1970T and type strains of known Catenovulum species were 56.6-57.1% while the percentage of conserved proteins (POCP) values between them were 28.5-31.5%.
33864544	5	36	theme	distinct	599:606	arg1	lineage					608:614	a distinct lineage	597:614	a distinct lineage close to the genus Catenovulum within the family Alteromonadaceae	597:680	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain SM1970T formed a distinct lineage close to the genus Catenovulum within the family Alteromonadaceae, sharing the highest sequence similarity (93.6%) with type strain of Catenovulum maritimum but < 93.0% sequence similarity with those of other known species in the class Gammaproteobacteria.
33864544	10	37	theme	40.1 mol	1588:1595	arg1	content					1558:1564	The genomic DNA G + C content	1536:1564	The genomic DNA G + C content of strain SM1970T	1536:1582	The genomic DNA G + C content of strain SM1970T was 40.1 mol%.
33864544	10	37	theme	40.1 mol	1588:1595	arg1	%					1596:1596	40.1 mol%	1588:1596	40.1 mol%	1588:1596	The genomic DNA G + C content of strain SM1970T was 40.1 mol%.
33864544	6	38	theme	major	877:881	arg1	acids					889:893	The major fatty acids	873:893	The major fatty acids of strain SM1970T	873:911	The major fatty acids of strain SM1970T were summed feature 3 (C16: 1 ω7c and/or C16: 1 ω6c), C16: 0 and summed feature 8 (C18: 1 ω7c and/or C18: 1 ω6c).
33864544	5	39	dep	%	783:783	arg1	93.0					779:782	93.0	779:782	93.0	779:782	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain SM1970T formed a distinct lineage close to the genus Catenovulum within the family Alteromonadaceae, sharing the highest sequence similarity (93.6%) with type strain of Catenovulum maritimum but < 93.0% sequence similarity with those of other known species in the class Gammaproteobacteria.
33864544	8	40	from	length	1259:1264	arg1	4,172,146 bp					1243:1254	4,172,146 bp	1243:1254	4,172,146 bp	1243:1254	The draft genome of strain SM1970T consisted of 77 scaffolds and was 4,172,146 bp in length, containing a complete set of genes for chitin degradation.
33864544	8	40	from	length	1259:1264	arg1	genome					1184:1189	The draft genome	1174:1189	The draft genome of strain SM1970T	1174:1207	The draft genome of strain SM1970T consisted of 77 scaffolds and was 4,172,146 bp in length, containing a complete set of genes for chitin degradation.
33864544	5	41	theme	strain	575:580	arg1	SM1970T					582:588	strain SM1970T	575:588	strain SM1970T	575:588	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain SM1970T formed a distinct lineage close to the genus Catenovulum within the family Alteromonadaceae, sharing the highest sequence similarity (93.6%) with type strain of Catenovulum maritimum but < 93.0% sequence similarity with those of other known species in the class Gammaproteobacteria.
33864544	11	42	theme	1K04323T = KCTC	1842:1856	arg1	SM1970T					1826:1832	SM1970T	1826:1832	SM1970T (= MCCC 1K04323T = KCTC 72844T)	1826:1864	On the basis of the polyphasic analysis, strain SM1970T is considered to represent a novel species in a novel genus of the family Alteromonadaceae, for which the name Marinifaba aquimaris is proposed with the type strain being SM1970T (= MCCC 1K04323T = KCTC 72844T).
33864544	11	42	theme	1K04323T = KCTC	1842:1856	arg1	72844T					1858:1863	= MCCC 1K04323T = KCTC 72844T	1835:1863	= MCCC 1K04323T = KCTC 72844T	1835:1863	On the basis of the polyphasic analysis, strain SM1970T is considered to represent a novel species in a novel genus of the family Alteromonadaceae, for which the name Marinifaba aquimaris is proposed with the type strain being SM1970T (= MCCC 1K04323T = KCTC 72844T).
33864544	5	43	theme	known	825:829	arg1	species					831:837	other known species	819:837	other known species in the class Gammaproteobacteria	819:870	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain SM1970T formed a distinct lineage close to the genus Catenovulum within the family Alteromonadaceae, sharing the highest sequence similarity (93.6%) with type strain of Catenovulum maritimum but < 93.0% sequence similarity with those of other known species in the class Gammaproteobacteria.
33864544	10	44	theme	genomic	1540:1546	arg1	content					1558:1564	The genomic DNA G + C content	1536:1564	The genomic DNA G + C content of strain SM1970T	1536:1582	The genomic DNA G + C content of strain SM1970T was 40.1 mol%.
33864544	10	44	theme	genomic	1540:1546	arg1	%					1596:1596	40.1 mol%	1588:1596	40.1 mol%	1588:1596	The genomic DNA G + C content of strain SM1970T was 40.1 mol%.
33864544	9	45	theme	amino	1338:1342	arg1	AAI					1359:1361	AAI	1359:1361	AAI	1359:1361	The average amino acid identity (AAI) values between SM1970T and type strains of known Catenovulum species were 56.6-57.1% while the percentage of conserved proteins (POCP) values between them were 28.5-31.5%.
33864544	9	45	theme	amino	1338:1342	arg1	identity					1349:1356	average amino acid identity	1330:1356	The average amino acid identity (AAI) values between SM1970T and type strains of known Catenovulum species	1326:1431	The average amino acid identity (AAI) values between SM1970T and type strains of known Catenovulum species were 56.6-57.1% while the percentage of conserved proteins (POCP) values between them were 28.5-31.5%.
33864544	9	46	theme	identity	1349:1356	arg1	values					1364:1369	The average amino acid identity (AAI) values	1326:1369	The average amino acid identity (AAI) values between SM1970T and type strains of known Catenovulum species	1326:1431	The average amino acid identity (AAI) values between SM1970T and type strains of known Catenovulum species were 56.6-57.1% while the percentage of conserved proteins (POCP) values between them were 28.5-31.5%.
33864544	9	46	theme	identity	1349:1356	arg1	%					1447:1447	56.6-57.1%	1438:1447	56.6-57.1%	1438:1447	The average amino acid identity (AAI) values between SM1970T and type strains of known Catenovulum species were 56.6-57.1% while the percentage of conserved proteins (POCP) values between them were 28.5-31.5%.
33864544	9	47	theme	known Catenovulum	1407:1423	arg1	species					1425:1431	known Catenovulum species	1407:1431	known Catenovulum species	1407:1431	The average amino acid identity (AAI) values between SM1970T and type strains of known Catenovulum species were 56.6-57.1% while the percentage of conserved proteins (POCP) values between them were 28.5-31.5%.
33864544	11	48	theme	novel	1703:1707	arg1	genus					1709:1713	a novel genus	1701:1713	a novel genus of the family Alteromonadaceae	1701:1744	On the basis of the polyphasic analysis, strain SM1970T is considered to represent a novel species in a novel genus of the family Alteromonadaceae, for which the name Marinifaba aquimaris is proposed with the type strain being SM1970T (= MCCC 1K04323T = KCTC 72844T).
33864544	11	49	theme	type	1808:1811	arg1	SM1970T					1826:1832	SM1970T	1826:1832	SM1970T (= MCCC 1K04323T = KCTC 72844T)	1826:1864	On the basis of the polyphasic analysis, strain SM1970T is considered to represent a novel species in a novel genus of the family Alteromonadaceae, for which the name Marinifaba aquimaris is proposed with the type strain being SM1970T (= MCCC 1K04323T = KCTC 72844T).
33864544	11	49	theme	type	1808:1811	arg1	strain					1813:1818	the type strain	1804:1818	the type strain	1804:1818	On the basis of the polyphasic analysis, strain SM1970T is considered to represent a novel species in a novel genus of the family Alteromonadaceae, for which the name Marinifaba aquimaris is proposed with the type strain being SM1970T (= MCCC 1K04323T = KCTC 72844T).
33864544	4	50	theme	colloidal	429:437	arg1	chitin					439:444	colloidal chitin	429:444	colloidal chitin	429:444	It hydrolyzed colloidal chitin, agar and casein but did not reduce nitrate to nitrite.
33864544	11	51	theme	novel	1684:1688	arg1	species					1690:1696	a novel species	1682:1696	a novel species	1682:1696	On the basis of the polyphasic analysis, strain SM1970T is considered to represent a novel species in a novel genus of the family Alteromonadaceae, for which the name Marinifaba aquimaris is proposed with the type strain being SM1970T (= MCCC 1K04323T = KCTC 72844T).
33864544	2	52	theme	oxidase-positive	209:224	arg1	bacterium					248:256	A novel Gram-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative bacterium	165:256	A novel Gram-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative bacterium	165:256	A novel Gram-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative bacterium, designated strain SM1970T, was isolated from a seawater sample collected from the Mariana Trench.
33864544	5	53	theme	Phylogenetic	502:513	arg1	analysis					515:522	Phylogenetic analysis	502:522	Phylogenetic analysis based on the 16S rRNA gene sequences	502:559	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain SM1970T formed a distinct lineage close to the genus Catenovulum within the family Alteromonadaceae, sharing the highest sequence similarity (93.6%) with type strain of Catenovulum maritimum but < 93.0% sequence similarity with those of other known species in the class Gammaproteobacteria.
33864544	6	54	theme	strain	898:903	arg1	SM1970T					905:911	strain SM1970T	898:911	strain SM1970T	898:911	The major fatty acids of strain SM1970T were summed feature 3 (C16: 1 ω7c and/or C16: 1 ω6c), C16: 0 and summed feature 8 (C18: 1 ω7c and/or C18: 1 ω6c).
33864544	6	55	dep	feature	925:931	arg1	C16					936:938	C16	936:938	C16	936:938	The major fatty acids of strain SM1970T were summed feature 3 (C16: 1 ω7c and/or C16: 1 ω6c), C16: 0 and summed feature 8 (C18: 1 ω7c and/or C18: 1 ω6c).
33864544	2	56	theme	strain	270:275	arg1	SM1970T					277:283	strain SM1970T	270:283	strain SM1970T	270:283	A novel Gram-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative bacterium, designated strain SM1970T, was isolated from a seawater sample collected from the Mariana Trench.
33864544	9	57	theme	SM1970T	1379:1385	arg1	strains					1396:1402	SM1970T and type strains	1379:1402	strains	1396:1402	The average amino acid identity (AAI) values between SM1970T and type strains of known Catenovulum species were 56.6-57.1% while the percentage of conserved proteins (POCP) values between them were 28.5-31.5%.
33864544	8	58	theme	strain	1194:1199	arg1	SM1970T					1201:1207	strain SM1970T	1194:1207	strain SM1970T	1194:1207	The draft genome of strain SM1970T consisted of 77 scaffolds and was 4,172,146 bp in length, containing a complete set of genes for chitin degradation.
33864544	5	59	theme	sequence	785:792	arg1	similarity					794:803	< 93.0% sequence similarity	777:803	< 93.0% sequence similarity	777:803	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain SM1970T formed a distinct lineage close to the genus Catenovulum within the family Alteromonadaceae, sharing the highest sequence similarity (93.6%) with type strain of Catenovulum maritimum but < 93.0% sequence similarity with those of other known species in the class Gammaproteobacteria.
33864544	9	60	theme	type	1391:1394	arg1	strains					1396:1402	SM1970T and type strains	1379:1402	strains	1396:1402	The average amino acid identity (AAI) values between SM1970T and type strains of known Catenovulum species were 56.6-57.1% while the percentage of conserved proteins (POCP) values between them were 28.5-31.5%.
33864544	1	61	theme	Mariana	149:155	arg1	Trench					157:162	the Mariana Trench	145:162	the Mariana Trench	145:162	nov., a novel chitin-degrading gammaproteobacterium in the family Alteromonadaceae isolated from seawater of the Mariana Trench.
33864544	8	62	theme	77	1222:1223	arg1	scaffolds					1225:1233	77 scaffolds	1222:1233	77 scaffolds	1222:1233	The draft genome of strain SM1970T consisted of 77 scaffolds and was 4,172,146 bp in length, containing a complete set of genes for chitin degradation.
33864544	5	63	theme	highest	695:701	arg1	similarity					712:721	the highest sequence similarity	691:721	the highest sequence similarity (93.6%) with type strain of Catenovulum maritimum	691:771	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain SM1970T formed a distinct lineage close to the genus Catenovulum within the family Alteromonadaceae, sharing the highest sequence similarity (93.6%) with type strain of Catenovulum maritimum but < 93.0% sequence similarity with those of other known species in the class Gammaproteobacteria.
33864544	5	63	theme	highest	695:701	arg1	%					728:728	93.6%	724:728	93.6%	724:728	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain SM1970T formed a distinct lineage close to the genus Catenovulum within the family Alteromonadaceae, sharing the highest sequence similarity (93.6%) with type strain of Catenovulum maritimum but < 93.0% sequence similarity with those of other known species in the class Gammaproteobacteria.
33864544	9	64	theme	species	1425:1431	arg1	strains					1396:1402	SM1970T and type strains	1379:1402	strains	1396:1402	The average amino acid identity (AAI) values between SM1970T and type strains of known Catenovulum species were 56.6-57.1% while the percentage of conserved proteins (POCP) values between them were 28.5-31.5%.
33864544	11	65	theme	polyphasic	1619:1628	arg1	analysis					1630:1637	the polyphasic analysis	1615:1637	the polyphasic analysis	1615:1637	On the basis of the polyphasic analysis, strain SM1970T is considered to represent a novel species in a novel genus of the family Alteromonadaceae, for which the name Marinifaba aquimaris is proposed with the type strain being SM1970T (= MCCC 1K04323T = KCTC 72844T).
33864544	7	66	theme	strain	1057:1062	arg1	lipids					1043:1048	The major polar lipids	1027:1048	The major polar lipids of the strain	1027:1062	The major polar lipids of the strain included phosphatidylethanolamine and phosphatidylglycerol and its main respiratory quinone was ubiquinone 8.
33864544	1	67	theme	novel	44:48	arg1	gammaproteobacterium					67:86	a novel chitin-degrading gammaproteobacterium	42:86	a novel chitin-degrading gammaproteobacterium in the family Alteromonadaceae isolated from seawater of the Mariana Trench	42:162	nov., a novel chitin-degrading gammaproteobacterium in the family Alteromonadaceae isolated from seawater of the Mariana Trench.
33864544	1	67	theme	novel	44:48	arg1	nov.					36:39	nov.	36:39	nov.	36:39	nov., a novel chitin-degrading gammaproteobacterium in the family Alteromonadaceae isolated from seawater of the Mariana Trench.
33864544	11	68	from	species	1690:1696	arg1	genus					1709:1713	a novel genus	1701:1713	a novel genus of the family Alteromonadaceae	1701:1744	On the basis of the polyphasic analysis, strain SM1970T is considered to represent a novel species in a novel genus of the family Alteromonadaceae, for which the name Marinifaba aquimaris is proposed with the type strain being SM1970T (= MCCC 1K04323T = KCTC 72844T).
33864544	0	69	theme	aquimaris	11:19	arg1	nov.					26:29	Marinifaba aquimaris gen. nov.	0:29	Marinifaba aquimaris gen. nov.	0:29	Marinifaba aquimaris gen. nov., sp.
33864544	6	70	theme	summed	978:983	arg1	feature					985:991	summed feature 8	978:993	summed feature 8 (C18: 1 ω7c and/or C18: 1 ω6c)	978:1024	The major fatty acids of strain SM1970T were summed feature 3 (C16: 1 ω7c and/or C16: 1 ω6c), C16: 0 and summed feature 8 (C18: 1 ω7c and/or C18: 1 ω6c).
33864544	3	71	dep	%	401:401	arg1	w/v					404:406	w/v	404:406	w/v	404:406	Strain SM1970T grew at 15-37 oC and with 1-5% (w/v) NaCl.
33864544	7	72	theme	polar	1037:1041	arg1	lipids					1043:1048	The major polar lipids	1027:1048	The major polar lipids of the strain	1027:1062	The major polar lipids of the strain included phosphatidylethanolamine and phosphatidylglycerol and its main respiratory quinone was ubiquinone 8.
33864544	5	73	theme	rRNA	541:544	arg1	sequences					551:559	the 16S rRNA gene sequences	533:559	the 16S rRNA gene sequences	533:559	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain SM1970T formed a distinct lineage close to the genus Catenovulum within the family Alteromonadaceae, sharing the highest sequence similarity (93.6%) with type strain of Catenovulum maritimum but < 93.0% sequence similarity with those of other known species in the class Gammaproteobacteria.
33864544	9	74	theme	percentage	1459:1468	arg1	values					1499:1504	the percentage of conserved proteins (POCP) values	1455:1504	the percentage of conserved proteins (POCP) values between them	1455:1517	The average amino acid identity (AAI) values between SM1970T and type strains of known Catenovulum species were 56.6-57.1% while the percentage of conserved proteins (POCP) values between them were 28.5-31.5%.
33864544	9	74	theme	percentage	1459:1468	arg1	POCP					1493:1496	POCP	1493:1496	POCP	1493:1496	The average amino acid identity (AAI) values between SM1970T and type strains of known Catenovulum species were 56.6-57.1% while the percentage of conserved proteins (POCP) values between them were 28.5-31.5%.
33864544	2	75	theme	catalase-negative	230:246	arg1	bacterium					248:256	A novel Gram-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative bacterium	165:256	A novel Gram-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative bacterium	165:256	A novel Gram-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative bacterium, designated strain SM1970T, was isolated from a seawater sample collected from the Mariana Trench.
33864544	2	76	theme	Gram-negative	173:185	arg1	bacterium					248:256	A novel Gram-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative bacterium	165:256	A novel Gram-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative bacterium	165:256	A novel Gram-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative bacterium, designated strain SM1970T, was isolated from a seawater sample collected from the Mariana Trench.
33864544	6	77	dep	feature	985:991	arg1	C18					996:998	C18	996:998	C18: 1 ω7c and/or C18: 1 ω6c	996:1023	The major fatty acids of strain SM1970T were summed feature 3 (C16: 1 ω7c and/or C16: 1 ω6c), C16: 0 and summed feature 8 (C18: 1 ω7c and/or C18: 1 ω6c).
33864544	10	78	theme	DNA	1548:1550	arg1	content					1558:1564	The genomic DNA G + C content	1536:1564	The genomic DNA G + C content of strain SM1970T	1536:1582	The genomic DNA G + C content of strain SM1970T was 40.1 mol%.
33864544	10	78	theme	DNA	1548:1550	arg1	%					1596:1596	40.1 mol%	1588:1596	40.1 mol%	1588:1596	The genomic DNA G + C content of strain SM1970T was 40.1 mol%.
33864544	5	79	theme	type	736:739	arg1	maritimum					763:771	type strain of Catenovulum maritimum	736:771	type strain of Catenovulum maritimum	736:771	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain SM1970T formed a distinct lineage close to the genus Catenovulum within the family Alteromonadaceae, sharing the highest sequence similarity (93.6%) with type strain of Catenovulum maritimum but < 93.0% sequence similarity with those of other known species in the class Gammaproteobacteria.
33864544	9	80	theme	conserved	1473:1481	arg1	proteins					1483:1490	conserved proteins	1473:1490	conserved proteins	1473:1490	The average amino acid identity (AAI) values between SM1970T and type strains of known Catenovulum species were 56.6-57.1% while the percentage of conserved proteins (POCP) values between them were 28.5-31.5%.
33864544	3	81	theme	%	401:401	arg1	NaCl					409:412	1-5% (w/v) NaCl	398:412	1-5% (w/v) NaCl	398:412	Strain SM1970T grew at 15-37 oC and with 1-5% (w/v) NaCl.
33864544	8	82	theme	complete	1280:1287	arg1	genes					1296:1300	genes	1296:1300	genes for chitin degradation	1296:1323	The draft genome of strain SM1970T consisted of 77 scaffolds and was 4,172,146 bp in length, containing a complete set of genes for chitin degradation.
33864544	8	82	theme	complete	1280:1287	arg1	set					1289:1291	a complete set	1278:1291	a complete set of genes for chitin degradation	1278:1323	The draft genome of strain SM1970T consisted of 77 scaffolds and was 4,172,146 bp in length, containing a complete set of genes for chitin degradation.
33864544	5	83	theme	of Catenovulum	748:761	arg1	maritimum					763:771	type strain of Catenovulum maritimum	736:771	type strain of Catenovulum maritimum	736:771	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain SM1970T formed a distinct lineage close to the genus Catenovulum within the family Alteromonadaceae, sharing the highest sequence similarity (93.6%) with type strain of Catenovulum maritimum but < 93.0% sequence similarity with those of other known species in the class Gammaproteobacteria.
33864544	5	84	from	species	831:837	arg1	class Gammaproteobacteria					846:870	the class Gammaproteobacteria	842:870	the class Gammaproteobacteria	842:870	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain SM1970T formed a distinct lineage close to the genus Catenovulum within the family Alteromonadaceae, sharing the highest sequence similarity (93.6%) with type strain of Catenovulum maritimum but < 93.0% sequence similarity with those of other known species in the class Gammaproteobacteria.
33864544	0	85	dep	sp	32:33	arg1	nov.					26:29	Marinifaba aquimaris gen. nov.	0:29	Marinifaba aquimaris gen. nov.	0:29	Marinifaba aquimaris gen. nov., sp.
33864544	2	86	theme	aerobic	200:206	arg1	bacterium					248:256	A novel Gram-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative bacterium	165:256	A novel Gram-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative bacterium	165:256	A novel Gram-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative bacterium, designated strain SM1970T, was isolated from a seawater sample collected from the Mariana Trench.
33864544	10	87	theme	strain	1569:1574	arg1	SM1970T					1576:1582	strain SM1970T	1569:1582	strain SM1970T	1569:1582	The genomic DNA G + C content of strain SM1970T was 40.1 mol%.
33864544	2	88	theme	rod-shaped	188:197	arg1	bacterium					248:256	A novel Gram-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative bacterium	165:256	A novel Gram-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative bacterium	165:256	A novel Gram-negative, rod-shaped, aerobic, oxidase-positive and catalase-negative bacterium, designated strain SM1970T, was isolated from a seawater sample collected from the Mariana Trench.
33864544	1	89	from	gammaproteobacterium	67:86	arg1	Alteromonadaceae					102:117	the family Alteromonadaceae	91:117	the family Alteromonadaceae isolated from seawater of the Mariana Trench	91:162	nov., a novel chitin-degrading gammaproteobacterium in the family Alteromonadaceae isolated from seawater of the Mariana Trench.
33864544	1	90	attach	isolated	119:126	arg2	Alteromonadaceae					102:117	the family Alteromonadaceae	91:117	the family Alteromonadaceae isolated from seawater of the Mariana Trench	91:162	nov., a novel chitin-degrading gammaproteobacterium in the family Alteromonadaceae isolated from seawater of the Mariana Trench.
33864544	1	90	attach	isolated	119:126	arg1	seawater					133:140	seawater	133:140	seawater of the Mariana Trench	133:162	nov., a novel chitin-degrading gammaproteobacterium in the family Alteromonadaceae isolated from seawater of the Mariana Trench.
33864544	5	91	dep	similarity	794:803	arg1	%					783:783	%	783:783	%	783:783	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain SM1970T formed a distinct lineage close to the genus Catenovulum within the family Alteromonadaceae, sharing the highest sequence similarity (93.6%) with type strain of Catenovulum maritimum but < 93.0% sequence similarity with those of other known species in the class Gammaproteobacteria.
33864544	11	92	theme	family Alteromonadaceae	1722:1744	arg1	genus					1709:1713	a novel genus	1701:1713	a novel genus of the family Alteromonadaceae	1701:1744	On the basis of the polyphasic analysis, strain SM1970T is considered to represent a novel species in a novel genus of the family Alteromonadaceae, for which the name Marinifaba aquimaris is proposed with the type strain being SM1970T (= MCCC 1K04323T = KCTC 72844T).
33864544	6	93	theme	SM1970T	905:911	arg1	acids					889:893	The major fatty acids	873:893	The major fatty acids of strain SM1970T	873:911	The major fatty acids of strain SM1970T were summed feature 3 (C16: 1 ω7c and/or C16: 1 ω6c), C16: 0 and summed feature 8 (C18: 1 ω7c and/or C18: 1 ω6c).
34602421	0	0	theme	polysaccharide	73:86	arg1	combination					28:38	the combination	24:38	the combination of aconitine and crude monkshood polysaccharide	24:86	The antitumor effect of the combination of aconitine and crude monkshood polysaccharide on hepatocellular carcinoma.
34602421	0	1	from	effect	14:19	arg1	carcinoma					106:114	hepatocellular carcinoma	91:114	hepatocellular carcinoma	91:114	The antitumor effect of the combination of aconitine and crude monkshood polysaccharide on hepatocellular carcinoma.
34602421	6	2	theme	immune	1179:1184	arg1	responses					1186:1194	the anti-tumor immune responses	1164:1194	the anti-tumor immune responses	1164:1194	Our results showed that the combination of aconitine and CMP exerts anti-tumor effect by directly killing tumor cells and enhancing the anti-tumor immune responses, which further implies that chemotherapy drugs combined with Chinese medicine immunopotentiator maybe a feasible and effective strategy for HCC.
34602421	1	3	theme	Radix	150:154	arg1	Preparata					174:182	Radix Aconiti Lateralis Preparata	150:182	Radix Aconiti Lateralis Preparata	150:182	Aconitine, the main component in Radix Aconiti Lateralis Preparata, not only exerts the anti-tumor effect on Hepatocellular Carcinoma (HCC) but also damages on immune system.
34602421	5	4	theme	CMP	991:993	arg1	combination					962:972	the combination	958:972	the combination of aconitine and CMP compare with control group (P<0.05)	958:1029	Elevated CD4+ T and CD8+ T cells and macrophages in spleen, decreased serum IL-6 level and increased serum IFN-γ and TNF-α levels were observed in mice treated with the combination of aconitine and CMP compare with control group (P<0.05).
34602421	0	5	theme	monkshood	63:71	arg1	polysaccharide					73:86	crude monkshood polysaccharide	57:86	crude monkshood polysaccharide	57:86	The antitumor effect of the combination of aconitine and crude monkshood polysaccharide on hepatocellular carcinoma.
34602421	6	6	theme	tumor	1138:1142	arg1	cells					1144:1148	tumor cells	1138:1148	tumor cells	1138:1148	Our results showed that the combination of aconitine and CMP exerts anti-tumor effect by directly killing tumor cells and enhancing the anti-tumor immune responses, which further implies that chemotherapy drugs combined with Chinese medicine immunopotentiator maybe a feasible and effective strategy for HCC.
34602421	2	7	theme	Monkshood	320:328	arg1	Polysaccharide					330:343	Crude Monkshood Polysaccharide	314:343	Crude Monkshood Polysaccharide (CMP)	314:349	In the present study, Crude Monkshood Polysaccharide (CMP), another one natural composition component originated from the same herbal with aconitine, combined with aconitine to investigate the effects on HCC and immunity in vitro and in vivo.
34602421	2	7	theme	Monkshood	320:328	arg1	component					384:392	another one natural composition component	352:392	another one natural composition component originated from the same herbal with aconitine	352:439	In the present study, Crude Monkshood Polysaccharide (CMP), another one natural composition component originated from the same herbal with aconitine, combined with aconitine to investigate the effects on HCC and immunity in vitro and in vivo.
34602421	2	7	theme	Monkshood	320:328	arg1	CMP					346:348	CMP	346:348	CMP	346:348	In the present study, Crude Monkshood Polysaccharide (CMP), another one natural composition component originated from the same herbal with aconitine, combined with aconitine to investigate the effects on HCC and immunity in vitro and in vivo.
34602421	0	8	theme	hepatocellular	91:104	arg1	carcinoma					106:114	hepatocellular carcinoma	91:114	hepatocellular carcinoma	91:114	The antitumor effect of the combination of aconitine and crude monkshood polysaccharide on hepatocellular carcinoma.
34602421	4	9	from	Aconitine-CMP	691:703	arg1	combination					708:718	combination	708:718	combination	708:718	Aconitine-CMP in combination improved the spleen weights, spleen index, thymus weights, thymus index.
34602421	5	10	theme	IL-6	869:872	arg1	level					874:878	decreased serum IL-6 level	853:878	decreased serum IL-6 level	853:878	Elevated CD4+ T and CD8+ T cells and macrophages in spleen, decreased serum IL-6 level and increased serum IFN-γ and TNF-α levels were observed in mice treated with the combination of aconitine and CMP compare with control group (P<0.05).
34602421	5	11	theme	serum	863:867	arg1	level					874:878	decreased serum IL-6 level	853:878	decreased serum IL-6 level	853:878	Elevated CD4+ T and CD8+ T cells and macrophages in spleen, decreased serum IL-6 level and increased serum IFN-γ and TNF-α levels were observed in mice treated with the combination of aconitine and CMP compare with control group (P<0.05).
34602421	6	12	theme	Chinese	1257:1263	arg1	maybe					1292:1296	Chinese medicine immunopotentiator maybe	1257:1296	Chinese medicine immunopotentiator maybe	1257:1296	Our results showed that the combination of aconitine and CMP exerts anti-tumor effect by directly killing tumor cells and enhancing the anti-tumor immune responses, which further implies that chemotherapy drugs combined with Chinese medicine immunopotentiator maybe a feasible and effective strategy for HCC.
34602421	3	13	theme	additive	654:661	arg1	effect					663:668	an additive effect	651:668	an additive effect	651:668	The combination of CMP and aconitine enhanced the ability of the immunocyte to kill the tumor cell in vitro and had an additive effect on anti-HCC in vivo.
34602421	1	14	from	component	137:145	arg1	Preparata					174:182	Radix Aconiti Lateralis Preparata	150:182	Radix Aconiti Lateralis Preparata	150:182	Aconitine, the main component in Radix Aconiti Lateralis Preparata, not only exerts the anti-tumor effect on Hepatocellular Carcinoma (HCC) but also damages on immune system.
34602421	5	15	theme	CD8+	813:816	arg1	cells					820:824	Elevated CD4+ T and CD8+ T cells	793:824	cells	820:824	Elevated CD4+ T and CD8+ T cells and macrophages in spleen, decreased serum IL-6 level and increased serum IFN-γ and TNF-α levels were observed in mice treated with the combination of aconitine and CMP compare with control group (P<0.05).
34602421	2	16	theme	present	299:305	arg1	study					307:311	the present study	295:311	the present study	295:311	In the present study, Crude Monkshood Polysaccharide (CMP), another one natural composition component originated from the same herbal with aconitine, combined with aconitine to investigate the effects on HCC and immunity in vitro and in vivo.
34602421	1	17	theme	Lateralis	164:172	arg1	Preparata					174:182	Radix Aconiti Lateralis Preparata	150:182	Radix Aconiti Lateralis Preparata	150:182	Aconitine, the main component in Radix Aconiti Lateralis Preparata, not only exerts the anti-tumor effect on Hepatocellular Carcinoma (HCC) but also damages on immune system.
34602421	5	18	theme	T	818:818	arg1	cells					820:824	Elevated CD4+ T and CD8+ T cells	793:824	cells	820:824	Elevated CD4+ T and CD8+ T cells and macrophages in spleen, decreased serum IL-6 level and increased serum IFN-γ and TNF-α levels were observed in mice treated with the combination of aconitine and CMP compare with control group (P<0.05).
34602421	4	19	theme	thymus	763:768	arg1	weights					740:746	the spleen weights	729:746	the spleen weights	729:746	Aconitine-CMP in combination improved the spleen weights, spleen index, thymus weights, thymus index.
34602421	4	19	theme	thymus	763:768	arg1	weights					770:776	thymus weights	763:776	thymus weights	763:776	Aconitine-CMP in combination improved the spleen weights, spleen index, thymus weights, thymus index.
34602421	5	20	from	macrophages	830:840	arg1	level					874:878	decreased serum IL-6 level	853:878	decreased serum IL-6 level	853:878	Elevated CD4+ T and CD8+ T cells and macrophages in spleen, decreased serum IL-6 level and increased serum IFN-γ and TNF-α levels were observed in mice treated with the combination of aconitine and CMP compare with control group (P<0.05).
34602421	5	20	from	macrophages	830:840	arg1	spleen					845:850	spleen	845:850	spleen	845:850	Elevated CD4+ T and CD8+ T cells and macrophages in spleen, decreased serum IL-6 level and increased serum IFN-γ and TNF-α levels were observed in mice treated with the combination of aconitine and CMP compare with control group (P<0.05).
34602421	5	20	from	macrophages	830:840	arg1	IFN-γ					900:904	increased serum IFN-γ and TNF-α levels	884:921	IFN-γ	900:904	Elevated CD4+ T and CD8+ T cells and macrophages in spleen, decreased serum IL-6 level and increased serum IFN-γ and TNF-α levels were observed in mice treated with the combination of aconitine and CMP compare with control group (P<0.05).
34602421	5	21	theme	increased	884:892	arg1	IFN-γ					900:904	increased serum IFN-γ and TNF-α levels	884:921	IFN-γ	900:904	Elevated CD4+ T and CD8+ T cells and macrophages in spleen, decreased serum IL-6 level and increased serum IFN-γ and TNF-α levels were observed in mice treated with the combination of aconitine and CMP compare with control group (P<0.05).
34602421	0	22	theme	antitumor	4:12	arg1	effect					14:19	The antitumor effect	0:19	The antitumor effect of the combination of aconitine and crude monkshood polysaccharide on hepatocellular carcinoma.	0:115	The antitumor effect of the combination of aconitine and crude monkshood polysaccharide on hepatocellular carcinoma.
34602421	5	23	theme	control	1008:1014	arg1	P<0.05					1023:1028	P<0.05	1023:1028	P<0.05	1023:1028	Elevated CD4+ T and CD8+ T cells and macrophages in spleen, decreased serum IL-6 level and increased serum IFN-γ and TNF-α levels were observed in mice treated with the combination of aconitine and CMP compare with control group (P<0.05).
34602421	5	23	theme	control	1008:1014	arg1	group					1016:1020	control group	1008:1020	control group (P<0.05)	1008:1029	Elevated CD4+ T and CD8+ T cells and macrophages in spleen, decreased serum IL-6 level and increased serum IFN-γ and TNF-α levels were observed in mice treated with the combination of aconitine and CMP compare with control group (P<0.05).
34602421	4	24	theme	thymus	779:784	arg1	weights					740:746	the spleen weights	729:746	the spleen weights	729:746	Aconitine-CMP in combination improved the spleen weights, spleen index, thymus weights, thymus index.
34602421	4	24	theme	thymus	779:784	arg1	index					786:790	thymus index	779:790	thymus index	779:790	Aconitine-CMP in combination improved the spleen weights, spleen index, thymus weights, thymus index.
34602421	6	25	theme	feasible	1300:1307	arg1	strategy					1323:1330	a feasible and effective strategy	1298:1330	a feasible and effective strategy for HCC	1298:1338	Our results showed that the combination of aconitine and CMP exerts anti-tumor effect by directly killing tumor cells and enhancing the anti-tumor immune responses, which further implies that chemotherapy drugs combined with Chinese medicine immunopotentiator maybe a feasible and effective strategy for HCC.
34602421	5	26	theme	Elevated	793:800	arg1	T					807:807	Elevated CD4+ T and CD8+ T cells	793:824	T	807:807	Elevated CD4+ T and CD8+ T cells and macrophages in spleen, decreased serum IL-6 level and increased serum IFN-γ and TNF-α levels were observed in mice treated with the combination of aconitine and CMP compare with control group (P<0.05).
34602421	2	27	theme	same	414:417	arg1	herbal					419:424	the same herbal	410:424	the same herbal with aconitine	410:439	In the present study, Crude Monkshood Polysaccharide (CMP), another one natural composition component originated from the same herbal with aconitine, combined with aconitine to investigate the effects on HCC and immunity in vitro and in vivo.
34602421	5	28	from	cells	820:824	arg1	level					874:878	decreased serum IL-6 level	853:878	decreased serum IL-6 level	853:878	Elevated CD4+ T and CD8+ T cells and macrophages in spleen, decreased serum IL-6 level and increased serum IFN-γ and TNF-α levels were observed in mice treated with the combination of aconitine and CMP compare with control group (P<0.05).
34602421	5	28	from	cells	820:824	arg1	spleen					845:850	spleen	845:850	spleen	845:850	Elevated CD4+ T and CD8+ T cells and macrophages in spleen, decreased serum IL-6 level and increased serum IFN-γ and TNF-α levels were observed in mice treated with the combination of aconitine and CMP compare with control group (P<0.05).
34602421	5	28	from	cells	820:824	arg1	IFN-γ					900:904	increased serum IFN-γ and TNF-α levels	884:921	IFN-γ	900:904	Elevated CD4+ T and CD8+ T cells and macrophages in spleen, decreased serum IL-6 level and increased serum IFN-γ and TNF-α levels were observed in mice treated with the combination of aconitine and CMP compare with control group (P<0.05).
34602421	5	29	theme	CD4+	802:805	arg1	T					807:807	Elevated CD4+ T and CD8+ T cells	793:824	T	807:807	Elevated CD4+ T and CD8+ T cells and macrophages in spleen, decreased serum IL-6 level and increased serum IFN-γ and TNF-α levels were observed in mice treated with the combination of aconitine and CMP compare with control group (P<0.05).
34602421	5	30	from	T	807:807	arg1	level					874:878	decreased serum IL-6 level	853:878	decreased serum IL-6 level	853:878	Elevated CD4+ T and CD8+ T cells and macrophages in spleen, decreased serum IL-6 level and increased serum IFN-γ and TNF-α levels were observed in mice treated with the combination of aconitine and CMP compare with control group (P<0.05).
34602421	5	30	from	T	807:807	arg1	spleen					845:850	spleen	845:850	spleen	845:850	Elevated CD4+ T and CD8+ T cells and macrophages in spleen, decreased serum IL-6 level and increased serum IFN-γ and TNF-α levels were observed in mice treated with the combination of aconitine and CMP compare with control group (P<0.05).
34602421	5	30	from	T	807:807	arg1	IFN-γ					900:904	increased serum IFN-γ and TNF-α levels	884:921	IFN-γ	900:904	Elevated CD4+ T and CD8+ T cells and macrophages in spleen, decreased serum IL-6 level and increased serum IFN-γ and TNF-α levels were observed in mice treated with the combination of aconitine and CMP compare with control group (P<0.05).
34602421	5	31	theme	decreased	853:861	arg1	level					874:878	decreased serum IL-6 level	853:878	decreased serum IL-6 level	853:878	Elevated CD4+ T and CD8+ T cells and macrophages in spleen, decreased serum IL-6 level and increased serum IFN-γ and TNF-α levels were observed in mice treated with the combination of aconitine and CMP compare with control group (P<0.05).
34602421	6	32	theme	chemotherapy	1224:1235	arg1	drugs					1237:1241	chemotherapy drugs	1224:1241	chemotherapy drugs	1224:1241	Our results showed that the combination of aconitine and CMP exerts anti-tumor effect by directly killing tumor cells and enhancing the anti-tumor immune responses, which further implies that chemotherapy drugs combined with Chinese medicine immunopotentiator maybe a feasible and effective strategy for HCC.
34602421	1	33	theme	Aconiti	156:162	arg1	Preparata					174:182	Radix Aconiti Lateralis Preparata	150:182	Radix Aconiti Lateralis Preparata	150:182	Aconitine, the main component in Radix Aconiti Lateralis Preparata, not only exerts the anti-tumor effect on Hepatocellular Carcinoma (HCC) but also damages on immune system.
34602421	0	34	theme	combination	28:38	arg1	effect					14:19	The antitumor effect	0:19	The antitumor effect of the combination of aconitine and crude monkshood polysaccharide on hepatocellular carcinoma.	0:115	The antitumor effect of the combination of aconitine and crude monkshood polysaccharide on hepatocellular carcinoma.
34602421	1	35	theme	immune	277:282	arg1	system					284:289	immune system	277:289	immune system	277:289	Aconitine, the main component in Radix Aconiti Lateralis Preparata, not only exerts the anti-tumor effect on Hepatocellular Carcinoma (HCC) but also damages on immune system.
34602421	3	36	theme	immunocyte	600:609	arg1	ability					585:591	the ability	581:591	the ability of the immunocyte to kill the tumor cell in vitro	581:641	The combination of CMP and aconitine enhanced the ability of the immunocyte to kill the tumor cell in vitro and had an additive effect on anti-HCC in vivo.
34602421	4	37	theme	spleen	749:754	arg1	weights					740:746	the spleen weights	729:746	the spleen weights	729:746	Aconitine-CMP in combination improved the spleen weights, spleen index, thymus weights, thymus index.
34602421	4	37	theme	spleen	749:754	arg1	index					756:760	spleen index	749:760	spleen index	749:760	Aconitine-CMP in combination improved the spleen weights, spleen index, thymus weights, thymus index.
34602421	6	38	theme	aconitine	1075:1083	arg1	combination					1060:1070	the combination	1056:1070	the combination of aconitine and CMP	1056:1091	Our results showed that the combination of aconitine and CMP exerts anti-tumor effect by directly killing tumor cells and enhancing the anti-tumor immune responses, which further implies that chemotherapy drugs combined with Chinese medicine immunopotentiator maybe a feasible and effective strategy for HCC.
34602421	2	39	theme	composition	372:382	arg1	Polysaccharide					330:343	Crude Monkshood Polysaccharide	314:343	Crude Monkshood Polysaccharide (CMP)	314:349	In the present study, Crude Monkshood Polysaccharide (CMP), another one natural composition component originated from the same herbal with aconitine, combined with aconitine to investigate the effects on HCC and immunity in vitro and in vivo.
34602421	2	39	theme	composition	372:382	arg1	component					384:392	another one natural composition component	352:392	another one natural composition component originated from the same herbal with aconitine	352:439	In the present study, Crude Monkshood Polysaccharide (CMP), another one natural composition component originated from the same herbal with aconitine, combined with aconitine to investigate the effects on HCC and immunity in vitro and in vivo.
34602421	5	40	theme	aconitine	977:985	arg1	combination					962:972	the combination	958:972	the combination of aconitine and CMP compare with control group (P<0.05)	958:1029	Elevated CD4+ T and CD8+ T cells and macrophages in spleen, decreased serum IL-6 level and increased serum IFN-γ and TNF-α levels were observed in mice treated with the combination of aconitine and CMP compare with control group (P<0.05).
34602421	3	41	theme	CMP	554:556	arg1	combination					539:549	The combination	535:549	The combination of CMP and aconitine	535:570	The combination of CMP and aconitine enhanced the ability of the immunocyte to kill the tumor cell in vitro and had an additive effect on anti-HCC in vivo.
34602421	1	42	theme	anti-tumor	205:214	arg1	effect					216:221	the anti-tumor effect	201:221	the anti-tumor effect	201:221	Aconitine, the main component in Radix Aconiti Lateralis Preparata, not only exerts the anti-tumor effect on Hepatocellular Carcinoma (HCC) but also damages on immune system.
34602421	0	43	theme	aconitine	43:51	arg1	combination					28:38	the combination	24:38	the combination of aconitine and crude monkshood polysaccharide	24:86	The antitumor effect of the combination of aconitine and crude monkshood polysaccharide on hepatocellular carcinoma.
34602421	6	44	theme	medicine	1265:1272	arg1	maybe					1292:1296	Chinese medicine immunopotentiator maybe	1257:1296	Chinese medicine immunopotentiator maybe	1257:1296	Our results showed that the combination of aconitine and CMP exerts anti-tumor effect by directly killing tumor cells and enhancing the anti-tumor immune responses, which further implies that chemotherapy drugs combined with Chinese medicine immunopotentiator maybe a feasible and effective strategy for HCC.
34602421	5	45	theme	TNF-α	910:914	arg1	levels					916:921	increased serum IFN-γ and TNF-α levels	884:921	levels	916:921	Elevated CD4+ T and CD8+ T cells and macrophages in spleen, decreased serum IL-6 level and increased serum IFN-γ and TNF-α levels were observed in mice treated with the combination of aconitine and CMP compare with control group (P<0.05).
34602421	2	46	theme	Crude	314:318	arg1	Polysaccharide					330:343	Crude Monkshood Polysaccharide	314:343	Crude Monkshood Polysaccharide (CMP)	314:349	In the present study, Crude Monkshood Polysaccharide (CMP), another one natural composition component originated from the same herbal with aconitine, combined with aconitine to investigate the effects on HCC and immunity in vitro and in vivo.
34602421	2	46	theme	Crude	314:318	arg1	component					384:392	another one natural composition component	352:392	another one natural composition component originated from the same herbal with aconitine	352:439	In the present study, Crude Monkshood Polysaccharide (CMP), another one natural composition component originated from the same herbal with aconitine, combined with aconitine to investigate the effects on HCC and immunity in vitro and in vivo.
34602421	2	46	theme	Crude	314:318	arg1	CMP					346:348	CMP	346:348	CMP	346:348	In the present study, Crude Monkshood Polysaccharide (CMP), another one natural composition component originated from the same herbal with aconitine, combined with aconitine to investigate the effects on HCC and immunity in vitro and in vivo.
34602421	2	47	from	effects	485:491	arg1	immunity					504:511	immunity	504:511	immunity	504:511	In the present study, Crude Monkshood Polysaccharide (CMP), another one natural composition component originated from the same herbal with aconitine, combined with aconitine to investigate the effects on HCC and immunity in vitro and in vivo.
34602421	2	47	from	effects	485:491	arg1	HCC					496:498	HCC	496:498	HCC	496:498	In the present study, Crude Monkshood Polysaccharide (CMP), another one natural composition component originated from the same herbal with aconitine, combined with aconitine to investigate the effects on HCC and immunity in vitro and in vivo.
34602421	6	48	theme	anti-tumor	1100:1109	arg1	effect					1111:1116	anti-tumor effect	1100:1116	anti-tumor effect	1100:1116	Our results showed that the combination of aconitine and CMP exerts anti-tumor effect by directly killing tumor cells and enhancing the anti-tumor immune responses, which further implies that chemotherapy drugs combined with Chinese medicine immunopotentiator maybe a feasible and effective strategy for HCC.
34602421	2	49	with	herbal	419:424	arg1	aconitine					431:439	aconitine	431:439	aconitine	431:439	In the present study, Crude Monkshood Polysaccharide (CMP), another one natural composition component originated from the same herbal with aconitine, combined with aconitine to investigate the effects on HCC and immunity in vitro and in vivo.
34602421	3	50	contain	had	647:649	arg2	effect					663:668	an additive effect	651:668	an additive effect	651:668	The combination of CMP and aconitine enhanced the ability of the immunocyte to kill the tumor cell in vitro and had an additive effect on anti-HCC in vivo.
34602421	3	50	contain	had	647:649	arg1	combination					539:549	The combination	535:549	The combination of CMP and aconitine	535:570	The combination of CMP and aconitine enhanced the ability of the immunocyte to kill the tumor cell in vitro and had an additive effect on anti-HCC in vivo.
34602421	4	51	theme	spleen	733:738	arg1	weights					740:746	the spleen weights	729:746	the spleen weights	729:746	Aconitine-CMP in combination improved the spleen weights, spleen index, thymus weights, thymus index.
34602421	4	51	theme	spleen	733:738	arg1	index					756:760	spleen index	749:760	spleen index	749:760	Aconitine-CMP in combination improved the spleen weights, spleen index, thymus weights, thymus index.
34602421	4	51	theme	spleen	733:738	arg1	weights					770:776	thymus weights	763:776	thymus weights	763:776	Aconitine-CMP in combination improved the spleen weights, spleen index, thymus weights, thymus index.
34602421	4	51	theme	spleen	733:738	arg1	index					786:790	thymus index	779:790	thymus index	779:790	Aconitine-CMP in combination improved the spleen weights, spleen index, thymus weights, thymus index.
34602421	6	52	theme	anti-tumor	1168:1177	arg1	responses					1186:1194	the anti-tumor immune responses	1164:1194	the anti-tumor immune responses	1164:1194	Our results showed that the combination of aconitine and CMP exerts anti-tumor effect by directly killing tumor cells and enhancing the anti-tumor immune responses, which further implies that chemotherapy drugs combined with Chinese medicine immunopotentiator maybe a feasible and effective strategy for HCC.
34602421	3	53	theme	tumor	623:627	arg1	cell					629:632	the tumor cell	619:632	the tumor cell	619:632	The combination of CMP and aconitine enhanced the ability of the immunocyte to kill the tumor cell in vitro and had an additive effect on anti-HCC in vivo.
34602421	5	54	theme	serum	894:898	arg1	IFN-γ					900:904	increased serum IFN-γ and TNF-α levels	884:921	IFN-γ	900:904	Elevated CD4+ T and CD8+ T cells and macrophages in spleen, decreased serum IL-6 level and increased serum IFN-γ and TNF-α levels were observed in mice treated with the combination of aconitine and CMP compare with control group (P<0.05).
34602421	5	55	dep	aconitine	977:985	arg1	compare					995:1001	compare	995:1001	compare with control group (P<0.05)	995:1029	Elevated CD4+ T and CD8+ T cells and macrophages in spleen, decreased serum IL-6 level and increased serum IFN-γ and TNF-α levels were observed in mice treated with the combination of aconitine and CMP compare with control group (P<0.05).
34602421	6	56	theme	CMP	1089:1091	arg1	combination					1060:1070	the combination	1056:1070	the combination of aconitine and CMP	1056:1091	Our results showed that the combination of aconitine and CMP exerts anti-tumor effect by directly killing tumor cells and enhancing the anti-tumor immune responses, which further implies that chemotherapy drugs combined with Chinese medicine immunopotentiator maybe a feasible and effective strategy for HCC.
34602421	1	57	theme	main	132:135	arg1	Aconitine					117:125	Aconitine	117:125	Aconitine	117:125	Aconitine, the main component in Radix Aconiti Lateralis Preparata, not only exerts the anti-tumor effect on Hepatocellular Carcinoma (HCC) but also damages on immune system.
34602421	1	57	theme	main	132:135	arg1	component					137:145	the main component	128:145	the main component in Radix Aconiti Lateralis Preparata	128:182	Aconitine, the main component in Radix Aconiti Lateralis Preparata, not only exerts the anti-tumor effect on Hepatocellular Carcinoma (HCC) but also damages on immune system.
34602421	0	58	theme	crude	57:61	arg1	polysaccharide					73:86	crude monkshood polysaccharide	57:86	crude monkshood polysaccharide	57:86	The antitumor effect of the combination of aconitine and crude monkshood polysaccharide on hepatocellular carcinoma.
34602421	2	59	theme	natural	364:370	arg1	Polysaccharide					330:343	Crude Monkshood Polysaccharide	314:343	Crude Monkshood Polysaccharide (CMP)	314:349	In the present study, Crude Monkshood Polysaccharide (CMP), another one natural composition component originated from the same herbal with aconitine, combined with aconitine to investigate the effects on HCC and immunity in vitro and in vivo.
34602421	2	59	theme	natural	364:370	arg1	component					384:392	another one natural composition component	352:392	another one natural composition component originated from the same herbal with aconitine	352:439	In the present study, Crude Monkshood Polysaccharide (CMP), another one natural composition component originated from the same herbal with aconitine, combined with aconitine to investigate the effects on HCC and immunity in vitro and in vivo.
34602421	6	60	theme	immunopotentiator	1274:1290	arg1	maybe					1292:1296	Chinese medicine immunopotentiator maybe	1257:1296	Chinese medicine immunopotentiator maybe	1257:1296	Our results showed that the combination of aconitine and CMP exerts anti-tumor effect by directly killing tumor cells and enhancing the anti-tumor immune responses, which further implies that chemotherapy drugs combined with Chinese medicine immunopotentiator maybe a feasible and effective strategy for HCC.
34602421	1	61	theme	Hepatocellular	226:239	arg1	HCC					252:254	HCC	252:254	HCC	252:254	Aconitine, the main component in Radix Aconiti Lateralis Preparata, not only exerts the anti-tumor effect on Hepatocellular Carcinoma (HCC) but also damages on immune system.
34602421	1	61	theme	Hepatocellular	226:239	arg1	Carcinoma					241:249	Hepatocellular Carcinoma	226:249	Hepatocellular Carcinoma (HCC)	226:255	Aconitine, the main component in Radix Aconiti Lateralis Preparata, not only exerts the anti-tumor effect on Hepatocellular Carcinoma (HCC) but also damages on immune system.
34602421	6	62	theme	effective	1313:1321	arg1	strategy					1323:1330	a feasible and effective strategy	1298:1330	a feasible and effective strategy for HCC	1298:1338	Our results showed that the combination of aconitine and CMP exerts anti-tumor effect by directly killing tumor cells and enhancing the anti-tumor immune responses, which further implies that chemotherapy drugs combined with Chinese medicine immunopotentiator maybe a feasible and effective strategy for HCC.
34602421	3	63	theme	aconitine	562:570	arg1	combination					539:549	The combination	535:549	The combination of CMP and aconitine	535:570	The combination of CMP and aconitine enhanced the ability of the immunocyte to kill the tumor cell in vitro and had an additive effect on anti-HCC in vivo.
34602421	5	64	located	observed	928:935	arg2	T					807:807	Elevated CD4+ T and CD8+ T cells	793:824	T	807:807	Elevated CD4+ T and CD8+ T cells and macrophages in spleen, decreased serum IL-6 level and increased serum IFN-γ and TNF-α levels were observed in mice treated with the combination of aconitine and CMP compare with control group (P<0.05).
34602421	5	64	located	observed	928:935	arg1	mice					940:943	mice	940:943	mice treated with the combination of aconitine and CMP compare with control group (P<0.05)	940:1029	Elevated CD4+ T and CD8+ T cells and macrophages in spleen, decreased serum IL-6 level and increased serum IFN-γ and TNF-α levels were observed in mice treated with the combination of aconitine and CMP compare with control group (P<0.05).
34602421	5	64	located	observed	928:935	arg2	macrophages					830:840	macrophages	830:840	macrophages	830:840	Elevated CD4+ T and CD8+ T cells and macrophages in spleen, decreased serum IL-6 level and increased serum IFN-γ and TNF-α levels were observed in mice treated with the combination of aconitine and CMP compare with control group (P<0.05).
34602421	5	64	located	observed	928:935	arg2	cells					820:824	Elevated CD4+ T and CD8+ T cells	793:824	cells	820:824	Elevated CD4+ T and CD8+ T cells and macrophages in spleen, decreased serum IL-6 level and increased serum IFN-γ and TNF-α levels were observed in mice treated with the combination of aconitine and CMP compare with control group (P<0.05).
33978398	6	0	from	day	1216:1218	arg1	survival					1204:1211	transplanted cell survival	1186:1211	transplanted cell survival at day 32 post transplantation	1186:1242	Hydrogel scaffolding of GRPs resulted in a 4.5-fold increase in transplanted cell survival at day 32 post transplantation compared to naked cells.
33978398	6	0	from	day	1216:1218	arg1	increase					1174:1181	a 4.5-fold increase	1163:1181	a 4.5-fold increase in transplanted cell survival at day 32 post transplantation	1163:1242	Hydrogel scaffolding of GRPs resulted in a 4.5-fold increase in transplanted cell survival at day 32 post transplantation compared to naked cells.
33978398	2	1	theme	initial	386:392	arg1	death					399:403	high initial cell death	381:403	high initial cell death	381:403	However, most central nervous system cell therapeutic paradigms are hampered by high initial cell death and a host anti-graft immune response.
33978398	8	2	theme	serial	1544:1549	arg1	imaging					1559:1565	serial in vivo imaging	1544:1565	serial in vivo imaging of cell survival and hydrogel degradation	1544:1607	Hence, hydrogel scaffolding of transplanted GRPs in conjunction with serial in vivo imaging of cell survival and hydrogel degradation has potential for further advances in glial cell therapy.
33978398	2	3	theme	central	315:321	arg1	system					331:336	system	331:336	system	331:336	However, most central nervous system cell therapeutic paradigms are hampered by high initial cell death and a host anti-graft immune response.
33978398	1	4	contain	have	164:167	arg2	potential					169:177	potential	169:177	potential to focally replace defunct astrocytes	169:215	Transplanted glial-restricted progenitor (GRP) cells have potential to focally replace defunct astrocytes and produce remyelinating oligodendrocytes to avert neuronal death and dysfunction.
33978398	1	4	contain	have	164:167	arg1	cells					158:162	Transplanted glial-restricted progenitor (GRP) cells	111:162	Transplanted glial-restricted progenitor (GRP) cells	111:162	Transplanted glial-restricted progenitor (GRP) cells have potential to focally replace defunct astrocytes and produce remyelinating oligodendrocytes to avert neuronal death and dysfunction.
33978398	8	5	dep	in	1551:1552	arg1	vivo					1554:1557	vivo	1554:1557	vivo	1554:1557	Hence, hydrogel scaffolding of transplanted GRPs in conjunction with serial in vivo imaging of cell survival and hydrogel degradation has potential for further advances in glial cell therapy.
33978398	4	6	theme	BALB/c	790:795	arg1	mice					797:800	immunocompetent BALB/c mice	774:800	immunocompetent BALB/c mice	774:800	Allogeneic GRPs expressing green fluorescent protein and firefly luciferase were scaffolded in optimized hydrogel formulations and transplanted intracerebrally into immunocompetent BALB/c mice followed by serial in vivo bioluminescent imaging and chemical exchange saturation transfer magnetic resonance imaging (CEST MRI).
33978398	2	7	theme	therapeutic	343:353	arg1	paradigms					355:363	most central nervous system cell therapeutic paradigms	310:363	most central nervous system cell therapeutic paradigms	310:363	However, most central nervous system cell therapeutic paradigms are hampered by high initial cell death and a host anti-graft immune response.
33978398	2	8	theme	system	331:336	arg1	paradigms					355:363	most central nervous system cell therapeutic paradigms	310:363	most central nervous system cell therapeutic paradigms	310:363	However, most central nervous system cell therapeutic paradigms are hampered by high initial cell death and a host anti-graft immune response.
33978398	5	9	theme	CEST	971:974	arg1	MRI					976:978	gelatin-sensitive CEST MRI	953:978	gelatin-sensitive CEST MRI	953:978	We demonstrate that gelatin-sensitive CEST MRI can be exploited to monitor hydrogel scaffold degradation in vivo for ∼5 weeks post transplantation without necessitating exogenous labeling.
33978398	8	10	theme	further	1627:1633	arg1	advances					1635:1642	further advances	1627:1642	further advances in glial cell therapy	1627:1664	Hence, hydrogel scaffolding of transplanted GRPs in conjunction with serial in vivo imaging of cell survival and hydrogel degradation has potential for further advances in glial cell therapy.
33978398	8	11	with	conjunction	1527:1537	arg1	imaging					1559:1565	serial in vivo imaging	1544:1565	serial in vivo imaging of cell survival and hydrogel degradation	1544:1607	Hence, hydrogel scaffolding of transplanted GRPs in conjunction with serial in vivo imaging of cell survival and hydrogel degradation has potential for further advances in glial cell therapy.
33978398	6	12	theme	4.5-fold	1165:1172	arg1	increase					1174:1181	a 4.5-fold increase	1163:1181	a 4.5-fold increase in transplanted cell survival at day 32 post transplantation	1163:1242	Hydrogel scaffolding of GRPs resulted in a 4.5-fold increase in transplanted cell survival at day 32 post transplantation compared to naked cells.
33978398	8	13	theme	cell	1653:1656	arg1	therapy					1658:1664	glial cell therapy	1647:1664	glial cell therapy	1647:1664	Hence, hydrogel scaffolding of transplanted GRPs in conjunction with serial in vivo imaging of cell survival and hydrogel degradation has potential for further advances in glial cell therapy.
33978398	8	14	theme	hydrogel	1482:1489	arg1	scaffolding					1491:1501	hydrogel scaffolding	1482:1501	hydrogel scaffolding of transplanted GRPs in conjunction with serial in vivo imaging of cell survival and hydrogel degradation	1482:1607	Hence, hydrogel scaffolding of transplanted GRPs in conjunction with serial in vivo imaging of cell survival and hydrogel degradation has potential for further advances in glial cell therapy.
33978398	7	15	theme	scaffolded	1397:1406	arg1	cells					1408:1412	scaffolded cells	1397:1412	scaffolded cells	1397:1412	Histological analysis showed significant enhancement of cell proliferation as well as Olig2+ and GFAP+ cell differentiation for scaffolded cells compared to naked cells, with reduced host immunoreactivity.
33978398	5	16	theme	scaffold	1017:1024	arg1	degradation					1026:1036	hydrogel scaffold degradation	1008:1036	hydrogel scaffold degradation	1008:1036	We demonstrate that gelatin-sensitive CEST MRI can be exploited to monitor hydrogel scaffold degradation in vivo for ∼5 weeks post transplantation without necessitating exogenous labeling.
33978398	4	17	theme	serial	814:819	arg1	imaging					844:850	serial in vivo bioluminescent imaging	814:850	serial in vivo bioluminescent imaging	814:850	Allogeneic GRPs expressing green fluorescent protein and firefly luciferase were scaffolded in optimized hydrogel formulations and transplanted intracerebrally into immunocompetent BALB/c mice followed by serial in vivo bioluminescent imaging and chemical exchange saturation transfer magnetic resonance imaging (CEST MRI).
33978398	0	18	theme	Hydrogel	77:84	arg1	Biodegradation					95:108	Hydrogel Scaffold Biodegradation	77:108	Hydrogel Scaffold Biodegradation	77:108	In Vivo Imaging of Allografted Glial-Restricted Progenitor Cell Survival and Hydrogel Scaffold Biodegradation.
33978398	6	19	theme	GRPs	1146:1149	arg1	scaffolding					1131:1141	Hydrogel scaffolding	1122:1141	Hydrogel scaffolding of GRPs	1122:1149	Hydrogel scaffolding of GRPs resulted in a 4.5-fold increase in transplanted cell survival at day 32 post transplantation compared to naked cells.
33978398	6	20	from	increase	1174:1181	arg1	day					1216:1218	day 32	1216:1221	day 32 post transplantation	1216:1242	Hydrogel scaffolding of GRPs resulted in a 4.5-fold increase in transplanted cell survival at day 32 post transplantation compared to naked cells.
33978398	6	20	from	increase	1174:1181	arg1	survival					1204:1211	transplanted cell survival	1186:1211	transplanted cell survival at day 32 post transplantation	1186:1242	Hydrogel scaffolding of GRPs resulted in a 4.5-fold increase in transplanted cell survival at day 32 post transplantation compared to naked cells.
33978398	4	21	theme	magnetic	894:901	arg1	MRI					927:929	CEST MRI	922:929	CEST MRI	922:929	Allogeneic GRPs expressing green fluorescent protein and firefly luciferase were scaffolded in optimized hydrogel formulations and transplanted intracerebrally into immunocompetent BALB/c mice followed by serial in vivo bioluminescent imaging and chemical exchange saturation transfer magnetic resonance imaging (CEST MRI).
33978398	4	21	theme	magnetic	894:901	arg1	imaging					913:919	magnetic resonance imaging	894:919	magnetic resonance imaging (CEST MRI)	894:930	Allogeneic GRPs expressing green fluorescent protein and firefly luciferase were scaffolded in optimized hydrogel formulations and transplanted intracerebrally into immunocompetent BALB/c mice followed by serial in vivo bioluminescent imaging and chemical exchange saturation transfer magnetic resonance imaging (CEST MRI).
33978398	1	22	theme	glial-restricted	124:139	arg1	cells					158:162	Transplanted glial-restricted progenitor (GRP) cells	111:162	Transplanted glial-restricted progenitor (GRP) cells	111:162	Transplanted glial-restricted progenitor (GRP) cells have potential to focally replace defunct astrocytes and produce remyelinating oligodendrocytes to avert neuronal death and dysfunction.
33978398	7	23	theme	GFAP+	1366:1370	arg1	differentiation					1377:1391	GFAP+ cell differentiation	1366:1391	GFAP+ cell differentiation	1366:1391	Histological analysis showed significant enhancement of cell proliferation as well as Olig2+ and GFAP+ cell differentiation for scaffolded cells compared to naked cells, with reduced host immunoreactivity.
33978398	7	24	dep	showed	1291:1296	arg1	compared					1414:1421	compared	1414:1421	compared	1414:1421	Histological analysis showed significant enhancement of cell proliferation as well as Olig2+ and GFAP+ cell differentiation for scaffolded cells compared to naked cells, with reduced host immunoreactivity.
33978398	2	25	theme	immune	427:432	arg1	response					434:441	a host anti-graft immune response	409:441	a host anti-graft immune response	409:441	However, most central nervous system cell therapeutic paradigms are hampered by high initial cell death and a host anti-graft immune response.
33978398	4	26	theme	chemical	856:863	arg1	transfer					885:892	chemical exchange saturation transfer	856:892	chemical exchange saturation transfer	856:892	Allogeneic GRPs expressing green fluorescent protein and firefly luciferase were scaffolded in optimized hydrogel formulations and transplanted intracerebrally into immunocompetent BALB/c mice followed by serial in vivo bioluminescent imaging and chemical exchange saturation transfer magnetic resonance imaging (CEST MRI).
33978398	2	27	theme	host	411:414	arg1	response					434:441	a host anti-graft immune response	409:441	a host anti-graft immune response	409:441	However, most central nervous system cell therapeutic paradigms are hampered by high initial cell death and a host anti-graft immune response.
33978398	0	28	theme	In	0:1	arg1	Imaging					8:14	In Vivo Imaging	0:14	In Vivo Imaging of Allografted Glial-Restricted Progenitor Cell Survival	0:71	In Vivo Imaging of Allografted Glial-Restricted Progenitor Cell Survival and Hydrogel Scaffold Biodegradation.
33978398	4	29	theme	saturation	874:883	arg1	transfer					885:892	chemical exchange saturation transfer	856:892	chemical exchange saturation transfer	856:892	Allogeneic GRPs expressing green fluorescent protein and firefly luciferase were scaffolded in optimized hydrogel formulations and transplanted intracerebrally into immunocompetent BALB/c mice followed by serial in vivo bioluminescent imaging and chemical exchange saturation transfer magnetic resonance imaging (CEST MRI).
33978398	7	30	theme	proliferation	1330:1342	arg1	enhancement					1310:1320	significant enhancement	1298:1320	significant enhancement of cell proliferation as well as Olig2+ and GFAP+ cell differentiation for scaffolded cells	1298:1412	Histological analysis showed significant enhancement of cell proliferation as well as Olig2+ and GFAP+ cell differentiation for scaffolded cells compared to naked cells, with reduced host immunoreactivity.
33978398	7	30	theme	proliferation	1330:1342	arg1	differentiation					1377:1391	GFAP+ cell differentiation	1366:1391	GFAP+ cell differentiation	1366:1391	Histological analysis showed significant enhancement of cell proliferation as well as Olig2+ and GFAP+ cell differentiation for scaffolded cells compared to naked cells, with reduced host immunoreactivity.
33978398	7	30	theme	proliferation	1330:1342	arg1	Olig2+					1355:1360	Olig2+	1355:1360	Olig2+	1355:1360	Histological analysis showed significant enhancement of cell proliferation as well as Olig2+ and GFAP+ cell differentiation for scaffolded cells compared to naked cells, with reduced host immunoreactivity.
33978398	4	31	dep	in	821:822	arg1	vivo					824:827	vivo	824:827	vivo	824:827	Allogeneic GRPs expressing green fluorescent protein and firefly luciferase were scaffolded in optimized hydrogel formulations and transplanted intracerebrally into immunocompetent BALB/c mice followed by serial in vivo bioluminescent imaging and chemical exchange saturation transfer magnetic resonance imaging (CEST MRI).
33978398	6	32	theme	cell	1199:1202	arg1	survival					1204:1211	transplanted cell survival	1186:1211	transplanted cell survival at day 32 post transplantation	1186:1242	Hydrogel scaffolding of GRPs resulted in a 4.5-fold increase in transplanted cell survival at day 32 post transplantation compared to naked cells.
33978398	4	33	theme	green	636:640	arg1	protein					654:660	green fluorescent protein	636:660	green fluorescent protein	636:660	Allogeneic GRPs expressing green fluorescent protein and firefly luciferase were scaffolded in optimized hydrogel formulations and transplanted intracerebrally into immunocompetent BALB/c mice followed by serial in vivo bioluminescent imaging and chemical exchange saturation transfer magnetic resonance imaging (CEST MRI).
33978398	6	34	theme	transplanted	1186:1197	arg1	survival					1204:1211	transplanted cell survival	1186:1211	transplanted cell survival at day 32 post transplantation	1186:1242	Hydrogel scaffolding of GRPs resulted in a 4.5-fold increase in transplanted cell survival at day 32 post transplantation compared to naked cells.
33978398	3	35	theme	acid-based	483:492	arg1	hydrogels					494:502	composite hyaluronic acid-based hydrogels	462:502	composite hyaluronic acid-based hydrogels of tunable mechanical strengths	462:534	We show here that composite hyaluronic acid-based hydrogels of tunable mechanical strengths can significantly improve transplanted GRP survival and differentiation.
33978398	0	36	theme	Allografted	19:29	arg1	Survival					64:71	Allografted Glial-Restricted Progenitor Cell Survival	19:71	Allografted Glial-Restricted Progenitor Cell Survival	19:71	In Vivo Imaging of Allografted Glial-Restricted Progenitor Cell Survival and Hydrogel Scaffold Biodegradation.
33978398	0	37	theme	Survival	64:71	arg1	Imaging					8:14	In Vivo Imaging	0:14	In Vivo Imaging of Allografted Glial-Restricted Progenitor Cell Survival	0:71	In Vivo Imaging of Allografted Glial-Restricted Progenitor Cell Survival and Hydrogel Scaffold Biodegradation.
33978398	0	37	theme	Survival	64:71	arg1	Biodegradation					95:108	Hydrogel Scaffold Biodegradation	77:108	Hydrogel Scaffold Biodegradation	77:108	In Vivo Imaging of Allografted Glial-Restricted Progenitor Cell Survival and Hydrogel Scaffold Biodegradation.
33978398	8	38	theme	survival	1575:1582	arg1	imaging					1559:1565	serial in vivo imaging	1544:1565	serial in vivo imaging of cell survival and hydrogel degradation	1544:1607	Hence, hydrogel scaffolding of transplanted GRPs in conjunction with serial in vivo imaging of cell survival and hydrogel degradation has potential for further advances in glial cell therapy.
33978398	2	39	theme	high	381:384	arg1	death					399:403	high initial cell death	381:403	high initial cell death	381:403	However, most central nervous system cell therapeutic paradigms are hampered by high initial cell death and a host anti-graft immune response.
33978398	0	40	theme	Progenitor	48:57	arg1	Survival					64:71	Allografted Glial-Restricted Progenitor Cell Survival	19:71	Allografted Glial-Restricted Progenitor Cell Survival	19:71	In Vivo Imaging of Allografted Glial-Restricted Progenitor Cell Survival and Hydrogel Scaffold Biodegradation.
33978398	7	41	theme	reduced	1444:1450	arg1	immunoreactivity					1457:1472	reduced host immunoreactivity	1444:1472	reduced host immunoreactivity	1444:1472	Histological analysis showed significant enhancement of cell proliferation as well as Olig2+ and GFAP+ cell differentiation for scaffolded cells compared to naked cells, with reduced host immunoreactivity.
33978398	8	42	theme	hydrogel	1588:1595	arg1	degradation					1597:1607	hydrogel degradation	1588:1607	hydrogel degradation	1588:1607	Hence, hydrogel scaffolding of transplanted GRPs in conjunction with serial in vivo imaging of cell survival and hydrogel degradation has potential for further advances in glial cell therapy.
33978398	3	43	theme	mechanical	515:524	arg1	strengths					526:534	tunable mechanical strengths	507:534	tunable mechanical strengths	507:534	We show here that composite hyaluronic acid-based hydrogels of tunable mechanical strengths can significantly improve transplanted GRP survival and differentiation.
33978398	3	44	theme	GRP	575:577	arg1	survival					579:586	transplanted GRP survival	562:586	transplanted GRP survival	562:586	We show here that composite hyaluronic acid-based hydrogels of tunable mechanical strengths can significantly improve transplanted GRP survival and differentiation.
33978398	1	45	theme	neuronal	269:276	arg1	death					278:282	neuronal death	269:282	neuronal death	269:282	Transplanted glial-restricted progenitor (GRP) cells have potential to focally replace defunct astrocytes and produce remyelinating oligodendrocytes to avert neuronal death and dysfunction.
33978398	0	46	dep	In	0:1	arg1	Vivo					3:6	Vivo	3:6	Vivo	3:6	In Vivo Imaging of Allografted Glial-Restricted Progenitor Cell Survival and Hydrogel Scaffold Biodegradation.
33978398	7	47	theme	naked	1426:1430	arg1	cells					1432:1436	naked cells	1426:1436	naked cells	1426:1436	Histological analysis showed significant enhancement of cell proliferation as well as Olig2+ and GFAP+ cell differentiation for scaffolded cells compared to naked cells, with reduced host immunoreactivity.
33978398	7	48	theme	cell	1372:1375	arg1	differentiation					1377:1391	GFAP+ cell differentiation	1366:1391	GFAP+ cell differentiation	1366:1391	Histological analysis showed significant enhancement of cell proliferation as well as Olig2+ and GFAP+ cell differentiation for scaffolded cells compared to naked cells, with reduced host immunoreactivity.
33978398	7	49	theme	Histological	1269:1280	arg1	analysis					1282:1289	Histological analysis	1269:1289	Histological analysis	1269:1289	Histological analysis showed significant enhancement of cell proliferation as well as Olig2+ and GFAP+ cell differentiation for scaffolded cells compared to naked cells, with reduced host immunoreactivity.
33978398	2	50	theme	cell	394:397	arg1	death					399:403	high initial cell death	381:403	high initial cell death	381:403	However, most central nervous system cell therapeutic paradigms are hampered by high initial cell death and a host anti-graft immune response.
33978398	2	51	theme	nervous	323:329	arg1	system					331:336	system	331:336	system	331:336	However, most central nervous system cell therapeutic paradigms are hampered by high initial cell death and a host anti-graft immune response.
33978398	4	52	theme	firefly	666:672	arg1	luciferase					674:683	firefly luciferase	666:683	firefly luciferase	666:683	Allogeneic GRPs expressing green fluorescent protein and firefly luciferase were scaffolded in optimized hydrogel formulations and transplanted intracerebrally into immunocompetent BALB/c mice followed by serial in vivo bioluminescent imaging and chemical exchange saturation transfer magnetic resonance imaging (CEST MRI).
33978398	7	53	theme	significant	1298:1308	arg1	enhancement					1310:1320	significant enhancement	1298:1320	significant enhancement of cell proliferation as well as Olig2+ and GFAP+ cell differentiation for scaffolded cells	1298:1412	Histological analysis showed significant enhancement of cell proliferation as well as Olig2+ and GFAP+ cell differentiation for scaffolded cells compared to naked cells, with reduced host immunoreactivity.
33978398	1	54	theme	defunct	198:204	arg1	astrocytes					206:215	defunct astrocytes	198:215	defunct astrocytes	198:215	Transplanted glial-restricted progenitor (GRP) cells have potential to focally replace defunct astrocytes and produce remyelinating oligodendrocytes to avert neuronal death and dysfunction.
33978398	6	55	theme	Hydrogel	1122:1129	arg1	scaffolding					1131:1141	Hydrogel scaffolding	1122:1141	Hydrogel scaffolding of GRPs	1122:1149	Hydrogel scaffolding of GRPs resulted in a 4.5-fold increase in transplanted cell survival at day 32 post transplantation compared to naked cells.
33978398	4	56	theme	immunocompetent	774:788	arg1	mice					797:800	immunocompetent BALB/c mice	774:800	immunocompetent BALB/c mice	774:800	Allogeneic GRPs expressing green fluorescent protein and firefly luciferase were scaffolded in optimized hydrogel formulations and transplanted intracerebrally into immunocompetent BALB/c mice followed by serial in vivo bioluminescent imaging and chemical exchange saturation transfer magnetic resonance imaging (CEST MRI).
33978398	2	57	theme	cell	338:341	arg1	paradigms					355:363	most central nervous system cell therapeutic paradigms	310:363	most central nervous system cell therapeutic paradigms	310:363	However, most central nervous system cell therapeutic paradigms are hampered by high initial cell death and a host anti-graft immune response.
33978398	5	58	theme	gelatin-sensitive	953:969	arg1	MRI					976:978	gelatin-sensitive CEST MRI	953:978	gelatin-sensitive CEST MRI	953:978	We demonstrate that gelatin-sensitive CEST MRI can be exploited to monitor hydrogel scaffold degradation in vivo for ∼5 weeks post transplantation without necessitating exogenous labeling.
33978398	4	59	theme	hydrogel	714:721	arg1	formulations					723:734	optimized hydrogel formulations	704:734	optimized hydrogel formulations	704:734	Allogeneic GRPs expressing green fluorescent protein and firefly luciferase were scaffolded in optimized hydrogel formulations and transplanted intracerebrally into immunocompetent BALB/c mice followed by serial in vivo bioluminescent imaging and chemical exchange saturation transfer magnetic resonance imaging (CEST MRI).
33978398	1	60	theme	remyelinating	229:241	arg1	oligodendrocytes					243:258	remyelinating oligodendrocytes	229:258	remyelinating oligodendrocytes	229:258	Transplanted glial-restricted progenitor (GRP) cells have potential to focally replace defunct astrocytes and produce remyelinating oligodendrocytes to avert neuronal death and dysfunction.
33978398	1	61	theme	progenitor	141:150	arg1	cells					158:162	Transplanted glial-restricted progenitor (GRP) cells	111:162	Transplanted glial-restricted progenitor (GRP) cells	111:162	Transplanted glial-restricted progenitor (GRP) cells have potential to focally replace defunct astrocytes and produce remyelinating oligodendrocytes to avert neuronal death and dysfunction.
33978398	8	62	theme	glial	1647:1651	arg1	therapy					1658:1664	glial cell therapy	1647:1664	glial cell therapy	1647:1664	Hence, hydrogel scaffolding of transplanted GRPs in conjunction with serial in vivo imaging of cell survival and hydrogel degradation has potential for further advances in glial cell therapy.
33978398	4	63	theme	in	821:822	arg1	imaging					844:850	serial in vivo bioluminescent imaging	814:850	serial in vivo bioluminescent imaging	814:850	Allogeneic GRPs expressing green fluorescent protein and firefly luciferase were scaffolded in optimized hydrogel formulations and transplanted intracerebrally into immunocompetent BALB/c mice followed by serial in vivo bioluminescent imaging and chemical exchange saturation transfer magnetic resonance imaging (CEST MRI).
33978398	4	64	theme	CEST	922:925	arg1	MRI					927:929	CEST MRI	922:929	CEST MRI	922:929	Allogeneic GRPs expressing green fluorescent protein and firefly luciferase were scaffolded in optimized hydrogel formulations and transplanted intracerebrally into immunocompetent BALB/c mice followed by serial in vivo bioluminescent imaging and chemical exchange saturation transfer magnetic resonance imaging (CEST MRI).
33978398	4	64	theme	CEST	922:925	arg1	imaging					913:919	magnetic resonance imaging	894:919	magnetic resonance imaging (CEST MRI)	894:930	Allogeneic GRPs expressing green fluorescent protein and firefly luciferase were scaffolded in optimized hydrogel formulations and transplanted intracerebrally into immunocompetent BALB/c mice followed by serial in vivo bioluminescent imaging and chemical exchange saturation transfer magnetic resonance imaging (CEST MRI).
33978398	1	65	theme	GRP	153:155	arg1	cells					158:162	Transplanted glial-restricted progenitor (GRP) cells	111:162	Transplanted glial-restricted progenitor (GRP) cells	111:162	Transplanted glial-restricted progenitor (GRP) cells have potential to focally replace defunct astrocytes and produce remyelinating oligodendrocytes to avert neuronal death and dysfunction.
33978398	8	66	theme	GRPs	1519:1522	arg1	scaffolding					1491:1501	hydrogel scaffolding	1482:1501	hydrogel scaffolding of transplanted GRPs in conjunction with serial in vivo imaging of cell survival and hydrogel degradation	1482:1607	Hence, hydrogel scaffolding of transplanted GRPs in conjunction with serial in vivo imaging of cell survival and hydrogel degradation has potential for further advances in glial cell therapy.
33978398	4	67	theme	bioluminescent	829:842	arg1	imaging					844:850	serial in vivo bioluminescent imaging	814:850	serial in vivo bioluminescent imaging	814:850	Allogeneic GRPs expressing green fluorescent protein and firefly luciferase were scaffolded in optimized hydrogel formulations and transplanted intracerebrally into immunocompetent BALB/c mice followed by serial in vivo bioluminescent imaging and chemical exchange saturation transfer magnetic resonance imaging (CEST MRI).
33978398	8	68	from	advances	1635:1642	arg1	therapy					1658:1664	glial cell therapy	1647:1664	glial cell therapy	1647:1664	Hence, hydrogel scaffolding of transplanted GRPs in conjunction with serial in vivo imaging of cell survival and hydrogel degradation has potential for further advances in glial cell therapy.
33978398	5	69	theme	hydrogel	1008:1015	arg1	degradation					1026:1036	hydrogel scaffold degradation	1008:1036	hydrogel scaffold degradation	1008:1036	We demonstrate that gelatin-sensitive CEST MRI can be exploited to monitor hydrogel scaffold degradation in vivo for ∼5 weeks post transplantation without necessitating exogenous labeling.
33978398	0	70	theme	Scaffold	86:93	arg1	Biodegradation					95:108	Hydrogel Scaffold Biodegradation	77:108	Hydrogel Scaffold Biodegradation	77:108	In Vivo Imaging of Allografted Glial-Restricted Progenitor Cell Survival and Hydrogel Scaffold Biodegradation.
33978398	6	71	dep	resulted	1151:1158	arg1	compared					1244:1251	compared	1244:1251	compared to naked cells	1244:1266	Hydrogel scaffolding of GRPs resulted in a 4.5-fold increase in transplanted cell survival at day 32 post transplantation compared to naked cells.
33978398	8	72	theme	in	1551:1552	arg1	imaging					1559:1565	serial in vivo imaging	1544:1565	serial in vivo imaging of cell survival and hydrogel degradation	1544:1607	Hence, hydrogel scaffolding of transplanted GRPs in conjunction with serial in vivo imaging of cell survival and hydrogel degradation has potential for further advances in glial cell therapy.
33978398	5	73	theme	exogenous	1102:1110	arg1	labeling					1112:1119	exogenous labeling	1102:1119	exogenous labeling	1102:1119	We demonstrate that gelatin-sensitive CEST MRI can be exploited to monitor hydrogel scaffold degradation in vivo for ∼5 weeks post transplantation without necessitating exogenous labeling.
33978398	8	74	theme	transplanted	1506:1517	arg1	GRPs					1519:1522	transplanted GRPs	1506:1522	transplanted GRPs	1506:1522	Hence, hydrogel scaffolding of transplanted GRPs in conjunction with serial in vivo imaging of cell survival and hydrogel degradation has potential for further advances in glial cell therapy.
33978398	4	75	theme	resonance	903:911	arg1	MRI					927:929	CEST MRI	922:929	CEST MRI	922:929	Allogeneic GRPs expressing green fluorescent protein and firefly luciferase were scaffolded in optimized hydrogel formulations and transplanted intracerebrally into immunocompetent BALB/c mice followed by serial in vivo bioluminescent imaging and chemical exchange saturation transfer magnetic resonance imaging (CEST MRI).
33978398	4	75	theme	resonance	903:911	arg1	imaging					913:919	magnetic resonance imaging	894:919	magnetic resonance imaging (CEST MRI)	894:930	Allogeneic GRPs expressing green fluorescent protein and firefly luciferase were scaffolded in optimized hydrogel formulations and transplanted intracerebrally into immunocompetent BALB/c mice followed by serial in vivo bioluminescent imaging and chemical exchange saturation transfer magnetic resonance imaging (CEST MRI).
33978398	2	76	theme	anti-graft	416:425	arg1	response					434:441	a host anti-graft immune response	409:441	a host anti-graft immune response	409:441	However, most central nervous system cell therapeutic paradigms are hampered by high initial cell death and a host anti-graft immune response.
33978398	8	77	from	scaffolding	1491:1501	arg1	conjunction					1527:1537	conjunction	1527:1537	conjunction with serial in vivo imaging of cell survival and hydrogel degradation	1527:1607	Hence, hydrogel scaffolding of transplanted GRPs in conjunction with serial in vivo imaging of cell survival and hydrogel degradation has potential for further advances in glial cell therapy.
33978398	4	78	dep	imaging	844:850	arg1	MRI					927:929	CEST MRI	922:929	CEST MRI	922:929	Allogeneic GRPs expressing green fluorescent protein and firefly luciferase were scaffolded in optimized hydrogel formulations and transplanted intracerebrally into immunocompetent BALB/c mice followed by serial in vivo bioluminescent imaging and chemical exchange saturation transfer magnetic resonance imaging (CEST MRI).
33978398	4	78	dep	imaging	844:850	arg1	imaging					913:919	magnetic resonance imaging	894:919	magnetic resonance imaging (CEST MRI)	894:930	Allogeneic GRPs expressing green fluorescent protein and firefly luciferase were scaffolded in optimized hydrogel formulations and transplanted intracerebrally into immunocompetent BALB/c mice followed by serial in vivo bioluminescent imaging and chemical exchange saturation transfer magnetic resonance imaging (CEST MRI).
33978398	8	79	contain	has	1609:1611	arg2	potential					1613:1621	potential	1613:1621	potential for further advances in glial cell therapy	1613:1664	Hence, hydrogel scaffolding of transplanted GRPs in conjunction with serial in vivo imaging of cell survival and hydrogel degradation has potential for further advances in glial cell therapy.
33978398	8	79	contain	has	1609:1611	arg1	scaffolding					1491:1501	hydrogel scaffolding	1482:1501	hydrogel scaffolding of transplanted GRPs in conjunction with serial in vivo imaging of cell survival and hydrogel degradation	1482:1607	Hence, hydrogel scaffolding of transplanted GRPs in conjunction with serial in vivo imaging of cell survival and hydrogel degradation has potential for further advances in glial cell therapy.
33978398	7	80	theme	cell	1325:1328	arg1	proliferation					1330:1342	cell proliferation	1325:1342	cell proliferation	1325:1342	Histological analysis showed significant enhancement of cell proliferation as well as Olig2+ and GFAP+ cell differentiation for scaffolded cells compared to naked cells, with reduced host immunoreactivity.
33978398	1	81	theme	Transplanted	111:122	arg1	cells					158:162	Transplanted glial-restricted progenitor (GRP) cells	111:162	Transplanted glial-restricted progenitor (GRP) cells	111:162	Transplanted glial-restricted progenitor (GRP) cells have potential to focally replace defunct astrocytes and produce remyelinating oligodendrocytes to avert neuronal death and dysfunction.
33978398	4	82	theme	fluorescent	642:652	arg1	protein					654:660	green fluorescent protein	636:660	green fluorescent protein	636:660	Allogeneic GRPs expressing green fluorescent protein and firefly luciferase were scaffolded in optimized hydrogel formulations and transplanted intracerebrally into immunocompetent BALB/c mice followed by serial in vivo bioluminescent imaging and chemical exchange saturation transfer magnetic resonance imaging (CEST MRI).
33978398	4	83	theme	exchange	865:872	arg1	transfer					885:892	chemical exchange saturation transfer	856:892	chemical exchange saturation transfer	856:892	Allogeneic GRPs expressing green fluorescent protein and firefly luciferase were scaffolded in optimized hydrogel formulations and transplanted intracerebrally into immunocompetent BALB/c mice followed by serial in vivo bioluminescent imaging and chemical exchange saturation transfer magnetic resonance imaging (CEST MRI).
33978398	3	84	theme	tunable	507:513	arg1	strengths					526:534	tunable mechanical strengths	507:534	tunable mechanical strengths	507:534	We show here that composite hyaluronic acid-based hydrogels of tunable mechanical strengths can significantly improve transplanted GRP survival and differentiation.
33978398	0	85	theme	Glial-Restricted	31:46	arg1	Survival					64:71	Allografted Glial-Restricted Progenitor Cell Survival	19:71	Allografted Glial-Restricted Progenitor Cell Survival	19:71	In Vivo Imaging of Allografted Glial-Restricted Progenitor Cell Survival and Hydrogel Scaffold Biodegradation.
33978398	4	86	dep	transplanted	740:751	arg1	followed					802:809	followed	802:809	followed by serial in vivo bioluminescent imaging and chemical exchange saturation transfer magnetic resonance imaging (CEST MRI)	802:930	Allogeneic GRPs expressing green fluorescent protein and firefly luciferase were scaffolded in optimized hydrogel formulations and transplanted intracerebrally into immunocompetent BALB/c mice followed by serial in vivo bioluminescent imaging and chemical exchange saturation transfer magnetic resonance imaging (CEST MRI).
33978398	7	87	theme	host	1452:1455	arg1	immunoreactivity					1457:1472	reduced host immunoreactivity	1444:1472	reduced host immunoreactivity	1444:1472	Histological analysis showed significant enhancement of cell proliferation as well as Olig2+ and GFAP+ cell differentiation for scaffolded cells compared to naked cells, with reduced host immunoreactivity.
33978398	3	88	theme	composite	462:470	arg1	hydrogels					494:502	composite hyaluronic acid-based hydrogels	462:502	composite hyaluronic acid-based hydrogels of tunable mechanical strengths	462:534	We show here that composite hyaluronic acid-based hydrogels of tunable mechanical strengths can significantly improve transplanted GRP survival and differentiation.
33978398	0	89	theme	Cell	59:62	arg1	Survival					64:71	Allografted Glial-Restricted Progenitor Cell Survival	19:71	Allografted Glial-Restricted Progenitor Cell Survival	19:71	In Vivo Imaging of Allografted Glial-Restricted Progenitor Cell Survival and Hydrogel Scaffold Biodegradation.
33978398	4	90	theme	Allogeneic	609:618	arg1	GRPs					620:623	Allogeneic GRPs	609:623	Allogeneic GRPs expressing green fluorescent protein and firefly luciferase	609:683	Allogeneic GRPs expressing green fluorescent protein and firefly luciferase were scaffolded in optimized hydrogel formulations and transplanted intracerebrally into immunocompetent BALB/c mice followed by serial in vivo bioluminescent imaging and chemical exchange saturation transfer magnetic resonance imaging (CEST MRI).
33978398	8	91	theme	cell	1570:1573	arg1	survival					1575:1582	cell survival	1570:1582	cell survival	1570:1582	Hence, hydrogel scaffolding of transplanted GRPs in conjunction with serial in vivo imaging of cell survival and hydrogel degradation has potential for further advances in glial cell therapy.
33978398	3	92	theme	transplanted	562:573	arg1	survival					579:586	transplanted GRP survival	562:586	transplanted GRP survival	562:586	We show here that composite hyaluronic acid-based hydrogels of tunable mechanical strengths can significantly improve transplanted GRP survival and differentiation.
33978398	3	93	theme	strengths	526:534	arg1	hydrogels					494:502	composite hyaluronic acid-based hydrogels	462:502	composite hyaluronic acid-based hydrogels of tunable mechanical strengths	462:534	We show here that composite hyaluronic acid-based hydrogels of tunable mechanical strengths can significantly improve transplanted GRP survival and differentiation.
33978398	6	94	theme	naked	1256:1260	arg1	cells					1262:1266	naked cells	1256:1266	naked cells	1256:1266	Hydrogel scaffolding of GRPs resulted in a 4.5-fold increase in transplanted cell survival at day 32 post transplantation compared to naked cells.
33978398	3	95	theme	hyaluronic	472:481	arg1	hydrogels					494:502	composite hyaluronic acid-based hydrogels	462:502	composite hyaluronic acid-based hydrogels of tunable mechanical strengths	462:534	We show here that composite hyaluronic acid-based hydrogels of tunable mechanical strengths can significantly improve transplanted GRP survival and differentiation.
33978398	4	96	theme	optimized	704:712	arg1	formulations					723:734	optimized hydrogel formulations	704:734	optimized hydrogel formulations	704:734	Allogeneic GRPs expressing green fluorescent protein and firefly luciferase were scaffolded in optimized hydrogel formulations and transplanted intracerebrally into immunocompetent BALB/c mice followed by serial in vivo bioluminescent imaging and chemical exchange saturation transfer magnetic resonance imaging (CEST MRI).
33978398	8	97	theme	degradation	1597:1607	arg1	imaging					1559:1565	serial in vivo imaging	1544:1565	serial in vivo imaging of cell survival and hydrogel degradation	1544:1607	Hence, hydrogel scaffolding of transplanted GRPs in conjunction with serial in vivo imaging of cell survival and hydrogel degradation has potential for further advances in glial cell therapy.
33164509	9	0	theme	content	1593:1599	arg1	monitoring					1574:1583	visual and real-time monitoring	1553:1583	visual and real-time monitoring of drug content	1553:1599	These synchronous thermoresponsive chromic drug uptake and release behaviors provided an effective method for visual and real-time monitoring of drug content.
33164509	7	1	theme	on-demand	1196:1204	arg1	release					1206:1212	on-demand release	1196:1212	on-demand release	1196:1212	The lower critical solution temperature (LCST) of the hydrogels was adjusted to 40 °C, which is higher than the skin temperature, by varying the content of hydrophilic comonomer acrylic acid (AAc) to store the drug at room temperature, and on-demand release was achieved by mild thermal stimulation.
33164509	3	2	theme	dosage	547:552	arg1	determination					525:537	the determination	521:537	the determination of drug dosage	521:552	In addition, real-time monitoring of drug content is also essential for guiding the determination of drug dosage and replacing drug carriers in time.
33164509	5	3	theme	photonic	690:697	arg1	PCs					709:711	PCs	709:711	PCs	709:711	Herein, photonic crystals (PCs) based on Fe3O4@C nanoparticles were introduced into drug-loaded poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAM-AAc)) hydrogel-functionalized textiles.
33164509	5	3	theme	photonic	690:697	arg1	crystals					699:706	photonic crystals	690:706	photonic crystals (PCs) based on Fe3O4@C nanoparticles	690:743	Herein, photonic crystals (PCs) based on Fe3O4@C nanoparticles were introduced into drug-loaded poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAM-AAc)) hydrogel-functionalized textiles.
33164509	10	4	theme	wound	1669:1673	arg1	dressings					1675:1683	hydrogel wound dressings	1660:1683	hydrogel wound dressings	1660:1683	Furthermore, in view of the poor mechanical properties of hydrogel wound dressings, textile matrices were composited to prevent holistic breaking during the stretching process.
33164509	2	5	theme	drug	311:314	arg1	systems					324:330	On-demand epidermal drug release systems	291:330	On-demand epidermal drug release systems	291:330	On-demand epidermal drug release systems can improve treatment efficiency, prevent multidrug resistance, and minimize drug toxicity to healthy cells.
33164509	8	6	dep	expansion	1313:1321	arg1	the					1309:1311	the	1309:1311	the	1309:1311	Moreover, the lattice spacing (d) of PCs varied with the expansion and contraction of the hydrogels, which can cause the color of P(NIPAM-AAc) hydrogel-functionalized textiles to change.
33164509	10	7	theme	holistic	1730:1737	arg1	breaking					1739:1746	holistic breaking	1730:1746	holistic breaking	1730:1746	Furthermore, in view of the poor mechanical properties of hydrogel wound dressings, textile matrices were composited to prevent holistic breaking during the stretching process.
33164509	1	8	theme	noninvasive	210:220	arg1	administration					249:262	noninvasive, convenient, and prolonged administration	210:262	noninvasive, convenient, and prolonged administration of pharmacological agents	210:288	In vitro drug release systems have recently received tremendous attention because they allow noninvasive, convenient, and prolonged administration of pharmacological agents.
33164509	5	9	dep	poly	778:781	arg1	NIPAM-AAc					825:833	NIPAM-AAc	825:833	NIPAM-AAc	825:833	Herein, photonic crystals (PCs) based on Fe3O4@C nanoparticles were introduced into drug-loaded poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAM-AAc)) hydrogel-functionalized textiles.
33164509	5	9	dep	poly	778:781	arg1	P					823:823	P	823:823	P(NIPAM-AAc)	823:834	Herein, photonic crystals (PCs) based on Fe3O4@C nanoparticles were introduced into drug-loaded poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAM-AAc)) hydrogel-functionalized textiles.
33164509	4	10	theme	ideal	659:663	arg1	patch					675:679	one ideal epidermal patch	655:679	one ideal epidermal patch	655:679	Therefore, it is important to integrate the above properties in one ideal epidermal patch.
33164509	10	11	theme	hydrogel	1660:1667	arg1	dressings					1675:1683	hydrogel wound dressings	1660:1683	hydrogel wound dressings	1660:1683	Furthermore, in view of the poor mechanical properties of hydrogel wound dressings, textile matrices were composited to prevent holistic breaking during the stretching process.
33164509	5	12	theme	hydrogel-functionalized	837:859	arg1	textiles					861:868	drug-loaded poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAM-AAc)) hydrogel-functionalized textiles	766:868	drug-loaded poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAM-AAc)) hydrogel-functionalized textiles	766:868	Herein, photonic crystals (PCs) based on Fe3O4@C nanoparticles were introduced into drug-loaded poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAM-AAc)) hydrogel-functionalized textiles.
33164509	11	13	theme	hydrogel-functionalized	1843:1865	arg1	textiles					1867:1874	the drug-loaded P(NIPAM-AAc) hydrogel-functionalized textiles	1814:1874	the drug-loaded P(NIPAM-AAc) hydrogel-functionalized textiles	1814:1874	Biological experiments proved that the drug-loaded P(NIPAM-AAc) hydrogel-functionalized textiles had good antibacterial properties and wound-healing effects.
33164509	5	14	theme	@	728:728	arg1	nanoparticles					731:743	Fe3O4@C nanoparticles	723:743	Fe3O4@C nanoparticles	723:743	Herein, photonic crystals (PCs) based on Fe3O4@C nanoparticles were introduced into drug-loaded poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAM-AAc)) hydrogel-functionalized textiles.
33164509	10	15	theme	stretching	1759:1768	arg1	process					1770:1776	the stretching process	1755:1776	the stretching process	1755:1776	Furthermore, in view of the poor mechanical properties of hydrogel wound dressings, textile matrices were composited to prevent holistic breaking during the stretching process.
33164509	7	16	theme	hydrogels	1010:1018	arg1	LCST					997:1000	LCST	997:1000	LCST	997:1000	The lower critical solution temperature (LCST) of the hydrogels was adjusted to 40 °C, which is higher than the skin temperature, by varying the content of hydrophilic comonomer acrylic acid (AAc) to store the drug at room temperature, and on-demand release was achieved by mild thermal stimulation.
33164509	7	16	theme	hydrogels	1010:1018	arg1	temperature					984:994	The lower critical solution temperature	956:994	The lower critical solution temperature (LCST) of the hydrogels	956:1018	The lower critical solution temperature (LCST) of the hydrogels was adjusted to 40 °C, which is higher than the skin temperature, by varying the content of hydrophilic comonomer acrylic acid (AAc) to store the drug at room temperature, and on-demand release was achieved by mild thermal stimulation.
33164509	1	17	theme	release	131:137	arg1	systems					139:145	In vitro drug release systems	117:145	In vitro drug release systems	117:145	In vitro drug release systems have recently received tremendous attention because they allow noninvasive, convenient, and prolonged administration of pharmacological agents.
33164509	6	18	theme	hydrogels	945:953	arg1	contraction					926:936	contraction	926:936	contraction	926:936	Drug loading and release depended on the expansion and contraction of the hydrogels.
33164509	6	18	theme	hydrogels	945:953	arg1	expansion					912:920	expansion	912:920	expansion	912:920	Drug loading and release depended on the expansion and contraction of the hydrogels.
33164509	1	19	theme	In	117:118	arg1	systems					139:145	In vitro drug release systems	117:145	In vitro drug release systems	117:145	In vitro drug release systems have recently received tremendous attention because they allow noninvasive, convenient, and prolonged administration of pharmacological agents.
33164509	2	20	theme	On-demand	291:299	arg1	systems					324:330	On-demand epidermal drug release systems	291:330	On-demand epidermal drug release systems	291:330	On-demand epidermal drug release systems can improve treatment efficiency, prevent multidrug resistance, and minimize drug toxicity to healthy cells.
33164509	1	21	dep	In	117:118	arg1	vitro					120:124	vitro	120:124	vitro	120:124	In vitro drug release systems have recently received tremendous attention because they allow noninvasive, convenient, and prolonged administration of pharmacological agents.
33164509	11	22	theme	good	1880:1883	arg1	properties					1899:1908	good antibacterial properties	1880:1908	good antibacterial properties	1880:1908	Biological experiments proved that the drug-loaded P(NIPAM-AAc) hydrogel-functionalized textiles had good antibacterial properties and wound-healing effects.
33164509	4	23	from	properties	641:650	arg1	patch					675:679	one ideal epidermal patch	655:679	one ideal epidermal patch	655:679	Therefore, it is important to integrate the above properties in one ideal epidermal patch.
33164509	9	24	theme	synchronous	1449:1459	arg1	behaviors					1510:1518	These synchronous thermoresponsive chromic drug uptake and release behaviors	1443:1518	These synchronous thermoresponsive chromic drug uptake and release behaviors	1443:1518	These synchronous thermoresponsive chromic drug uptake and release behaviors provided an effective method for visual and real-time monitoring of drug content.
33164509	8	25	theme	textiles	1423:1430	arg1	color					1377:1381	the color	1373:1381	the color of P(NIPAM-AAc) hydrogel-functionalized textiles to change	1373:1440	Moreover, the lattice spacing (d) of PCs varied with the expansion and contraction of the hydrogels, which can cause the color of P(NIPAM-AAc) hydrogel-functionalized textiles to change.
33164509	3	26	from	carriers	573:580	arg1	time					585:588	time	585:588	time	585:588	In addition, real-time monitoring of drug content is also essential for guiding the determination of drug dosage and replacing drug carriers in time.
33164509	9	27	theme	chromic	1478:1484	arg1	uptake					1491:1496	thermoresponsive chromic drug uptake	1461:1496	thermoresponsive chromic drug uptake	1461:1496	These synchronous thermoresponsive chromic drug uptake and release behaviors provided an effective method for visual and real-time monitoring of drug content.
33164509	7	28	theme	room	1174:1177	arg1	temperature					1179:1189	room temperature	1174:1189	room temperature	1174:1189	The lower critical solution temperature (LCST) of the hydrogels was adjusted to 40 °C, which is higher than the skin temperature, by varying the content of hydrophilic comonomer acrylic acid (AAc) to store the drug at room temperature, and on-demand release was achieved by mild thermal stimulation.
33164509	7	29	theme	critical	966:973	arg1	LCST					997:1000	LCST	997:1000	LCST	997:1000	The lower critical solution temperature (LCST) of the hydrogels was adjusted to 40 °C, which is higher than the skin temperature, by varying the content of hydrophilic comonomer acrylic acid (AAc) to store the drug at room temperature, and on-demand release was achieved by mild thermal stimulation.
33164509	7	29	theme	critical	966:973	arg1	temperature					984:994	The lower critical solution temperature	956:994	The lower critical solution temperature (LCST) of the hydrogels	956:1018	The lower critical solution temperature (LCST) of the hydrogels was adjusted to 40 °C, which is higher than the skin temperature, by varying the content of hydrophilic comonomer acrylic acid (AAc) to store the drug at room temperature, and on-demand release was achieved by mild thermal stimulation.
33164509	9	30	theme	uptake	1491:1496	arg1	behaviors					1510:1518	These synchronous thermoresponsive chromic drug uptake and release behaviors	1443:1518	These synchronous thermoresponsive chromic drug uptake and release behaviors	1443:1518	These synchronous thermoresponsive chromic drug uptake and release behaviors provided an effective method for visual and real-time monitoring of drug content.
33164509	3	31	theme	drug	478:481	arg1	content					483:489	drug content	478:489	drug content	478:489	In addition, real-time monitoring of drug content is also essential for guiding the determination of drug dosage and replacing drug carriers in time.
33164509	7	32	theme	acrylic	1134:1140	arg1	AAc					1148:1150	AAc	1148:1150	AAc	1148:1150	The lower critical solution temperature (LCST) of the hydrogels was adjusted to 40 °C, which is higher than the skin temperature, by varying the content of hydrophilic comonomer acrylic acid (AAc) to store the drug at room temperature, and on-demand release was achieved by mild thermal stimulation.
33164509	7	32	theme	acrylic	1134:1140	arg1	acid					1142:1145	hydrophilic comonomer acrylic acid	1112:1145	hydrophilic comonomer acrylic acid (AAc)	1112:1151	The lower critical solution temperature (LCST) of the hydrogels was adjusted to 40 °C, which is higher than the skin temperature, by varying the content of hydrophilic comonomer acrylic acid (AAc) to store the drug at room temperature, and on-demand release was achieved by mild thermal stimulation.
33164509	0	33	dep	Release	108:114	arg1	Textiles					38:45	Thermochromic Hydrogel-Functionalized Textiles	0:45	Thermochromic Hydrogel-Functionalized Textiles for Synchronous Visual Monitoring of On-Demand	0:92	Thermochromic Hydrogel-Functionalized Textiles for Synchronous Visual Monitoring of On-Demand In Vitro Drug Release.
33164509	2	34	theme	healthy	426:432	arg1	cells					434:438	healthy cells	426:438	healthy cells	426:438	On-demand epidermal drug release systems can improve treatment efficiency, prevent multidrug resistance, and minimize drug toxicity to healthy cells.
33164509	0	35	theme	Thermochromic	0:12	arg1	Textiles					38:45	Thermochromic Hydrogel-Functionalized Textiles	0:45	Thermochromic Hydrogel-Functionalized Textiles for Synchronous Visual Monitoring of On-Demand	0:92	Thermochromic Hydrogel-Functionalized Textiles for Synchronous Visual Monitoring of On-Demand In Vitro Drug Release.
33164509	11	36	theme	wound-healing	1914:1926	arg1	effects					1928:1934	wound-healing effects	1914:1934	wound-healing effects	1914:1934	Biological experiments proved that the drug-loaded P(NIPAM-AAc) hydrogel-functionalized textiles had good antibacterial properties and wound-healing effects.
33164509	3	37	theme	drug	568:571	arg1	carriers					573:580	drug carriers	568:580	drug carriers in time	568:588	In addition, real-time monitoring of drug content is also essential for guiding the determination of drug dosage and replacing drug carriers in time.
33164509	9	38	theme	effective	1532:1540	arg1	method					1542:1547	an effective method	1529:1547	an effective method for visual and real-time monitoring of drug content	1529:1599	These synchronous thermoresponsive chromic drug uptake and release behaviors provided an effective method for visual and real-time monitoring of drug content.
33164509	0	39	theme	On-Demand	84:92	arg1	Monitoring					70:79	Synchronous Visual Monitoring	51:79	Synchronous Visual Monitoring of On-Demand	51:92	Thermochromic Hydrogel-Functionalized Textiles for Synchronous Visual Monitoring of On-Demand In Vitro Drug Release.
33164509	7	40	theme	acid	1142:1145	arg1	content					1101:1107	the content	1097:1107	the content of hydrophilic comonomer acrylic acid (AAc) to store the drug at room temperature	1097:1189	The lower critical solution temperature (LCST) of the hydrogels was adjusted to 40 °C, which is higher than the skin temperature, by varying the content of hydrophilic comonomer acrylic acid (AAc) to store the drug at room temperature, and on-demand release was achieved by mild thermal stimulation.
33164509	0	41	theme	Synchronous	51:61	arg1	Monitoring					70:79	Synchronous Visual Monitoring	51:79	Synchronous Visual Monitoring of On-Demand	51:92	Thermochromic Hydrogel-Functionalized Textiles for Synchronous Visual Monitoring of On-Demand In Vitro Drug Release.
33164509	6	42	dep	Drug	871:874	arg1	loading					876:882	loading	876:882	loading	876:882	Drug loading and release depended on the expansion and contraction of the hydrogels.
33164509	5	43	theme	drug-loaded	766:776	arg1	poly					778:781	drug-loaded poly	766:781	drug-loaded poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAM-AAc)) hydrogel-functionalized textiles	766:868	Herein, photonic crystals (PCs) based on Fe3O4@C nanoparticles were introduced into drug-loaded poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAM-AAc)) hydrogel-functionalized textiles.
33164509	5	43	theme	drug-loaded	766:776	arg1	acid					816:819	N-isopropylacrylamide-co-acrylic acid	783:819	N-isopropylacrylamide-co-acrylic acid	783:819	Herein, photonic crystals (PCs) based on Fe3O4@C nanoparticles were introduced into drug-loaded poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAM-AAc)) hydrogel-functionalized textiles.
33164509	8	44	theme	hydrogels	1346:1354	arg1	contraction					1327:1337	contraction	1327:1337	contraction	1327:1337	Moreover, the lattice spacing (d) of PCs varied with the expansion and contraction of the hydrogels, which can cause the color of P(NIPAM-AAc) hydrogel-functionalized textiles to change.
33164509	8	44	theme	hydrogels	1346:1354	arg1	expansion					1313:1321	expansion	1313:1321	expansion	1313:1321	Moreover, the lattice spacing (d) of PCs varied with the expansion and contraction of the hydrogels, which can cause the color of P(NIPAM-AAc) hydrogel-functionalized textiles to change.
33164509	11	45	theme	drug-loaded	1818:1828	arg1	textiles					1867:1874	the drug-loaded P(NIPAM-AAc) hydrogel-functionalized textiles	1814:1874	the drug-loaded P(NIPAM-AAc) hydrogel-functionalized textiles	1814:1874	Biological experiments proved that the drug-loaded P(NIPAM-AAc) hydrogel-functionalized textiles had good antibacterial properties and wound-healing effects.
33164509	7	46	theme	comonomer	1124:1132	arg1	AAc					1148:1150	AAc	1148:1150	AAc	1148:1150	The lower critical solution temperature (LCST) of the hydrogels was adjusted to 40 °C, which is higher than the skin temperature, by varying the content of hydrophilic comonomer acrylic acid (AAc) to store the drug at room temperature, and on-demand release was achieved by mild thermal stimulation.
33164509	7	46	theme	comonomer	1124:1132	arg1	acid					1142:1145	hydrophilic comonomer acrylic acid	1112:1145	hydrophilic comonomer acrylic acid (AAc)	1112:1151	The lower critical solution temperature (LCST) of the hydrogels was adjusted to 40 °C, which is higher than the skin temperature, by varying the content of hydrophilic comonomer acrylic acid (AAc) to store the drug at room temperature, and on-demand release was achieved by mild thermal stimulation.
33164509	2	47	theme	multidrug	374:382	arg1	resistance					384:393	multidrug resistance	374:393	multidrug resistance	374:393	On-demand epidermal drug release systems can improve treatment efficiency, prevent multidrug resistance, and minimize drug toxicity to healthy cells.
33164509	1	48	theme	prolonged	239:247	arg1	administration					249:262	noninvasive, convenient, and prolonged administration	210:262	noninvasive, convenient, and prolonged administration of pharmacological agents	210:288	In vitro drug release systems have recently received tremendous attention because they allow noninvasive, convenient, and prolonged administration of pharmacological agents.
33164509	10	49	theme	textile	1686:1692	arg1	matrices					1694:1701	textile matrices	1686:1701	textile matrices	1686:1701	Furthermore, in view of the poor mechanical properties of hydrogel wound dressings, textile matrices were composited to prevent holistic breaking during the stretching process.
33164509	7	50	theme	mild	1230:1233	arg1	stimulation					1243:1253	mild thermal stimulation	1230:1253	mild thermal stimulation	1230:1253	The lower critical solution temperature (LCST) of the hydrogels was adjusted to 40 °C, which is higher than the skin temperature, by varying the content of hydrophilic comonomer acrylic acid (AAc) to store the drug at room temperature, and on-demand release was achieved by mild thermal stimulation.
33164509	9	51	theme	drug	1588:1591	arg1	content					1593:1599	drug content	1588:1599	drug content	1588:1599	These synchronous thermoresponsive chromic drug uptake and release behaviors provided an effective method for visual and real-time monitoring of drug content.
33164509	5	52	theme	poly	778:781	arg1	textiles					861:868	drug-loaded poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAM-AAc)) hydrogel-functionalized textiles	766:868	drug-loaded poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAM-AAc)) hydrogel-functionalized textiles	766:868	Herein, photonic crystals (PCs) based on Fe3O4@C nanoparticles were introduced into drug-loaded poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAM-AAc)) hydrogel-functionalized textiles.
33164509	3	53	theme	drug	542:545	arg1	dosage					547:552	drug dosage	542:552	drug dosage	542:552	In addition, real-time monitoring of drug content is also essential for guiding the determination of drug dosage and replacing drug carriers in time.
33164509	1	54	theme	agents	283:288	arg1	administration					249:262	noninvasive, convenient, and prolonged administration	210:262	noninvasive, convenient, and prolonged administration of pharmacological agents	210:288	In vitro drug release systems have recently received tremendous attention because they allow noninvasive, convenient, and prolonged administration of pharmacological agents.
33164509	1	55	theme	convenient	223:232	arg1	administration					249:262	noninvasive, convenient, and prolonged administration	210:262	noninvasive, convenient, and prolonged administration of pharmacological agents	210:288	In vitro drug release systems have recently received tremendous attention because they allow noninvasive, convenient, and prolonged administration of pharmacological agents.
33164509	9	56	theme	thermoresponsive	1461:1476	arg1	uptake					1491:1496	thermoresponsive chromic drug uptake	1461:1496	thermoresponsive chromic drug uptake	1461:1496	These synchronous thermoresponsive chromic drug uptake and release behaviors provided an effective method for visual and real-time monitoring of drug content.
33164509	2	57	theme	release	316:322	arg1	systems					324:330	On-demand epidermal drug release systems	291:330	On-demand epidermal drug release systems	291:330	On-demand epidermal drug release systems can improve treatment efficiency, prevent multidrug resistance, and minimize drug toxicity to healthy cells.
33164509	7	58	theme	skin	1068:1071	arg1	temperature					1073:1083	the skin temperature	1064:1083	the skin temperature	1064:1083	The lower critical solution temperature (LCST) of the hydrogels was adjusted to 40 °C, which is higher than the skin temperature, by varying the content of hydrophilic comonomer acrylic acid (AAc) to store the drug at room temperature, and on-demand release was achieved by mild thermal stimulation.
33164509	2	59	theme	treatment	344:352	arg1	efficiency					354:363	treatment efficiency	344:363	treatment efficiency	344:363	On-demand epidermal drug release systems can improve treatment efficiency, prevent multidrug resistance, and minimize drug toxicity to healthy cells.
33164509	11	60	theme	P	1830:1830	arg1	textiles					1867:1874	the drug-loaded P(NIPAM-AAc) hydrogel-functionalized textiles	1814:1874	the drug-loaded P(NIPAM-AAc) hydrogel-functionalized textiles	1814:1874	Biological experiments proved that the drug-loaded P(NIPAM-AAc) hydrogel-functionalized textiles had good antibacterial properties and wound-healing effects.
33164509	5	61	theme	Fe3O4	723:727	arg1	nanoparticles					731:743	Fe3O4@C nanoparticles	723:743	Fe3O4@C nanoparticles	723:743	Herein, photonic crystals (PCs) based on Fe3O4@C nanoparticles were introduced into drug-loaded poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAM-AAc)) hydrogel-functionalized textiles.
33164509	4	62	theme	epidermal	665:673	arg1	patch					675:679	one ideal epidermal patch	655:679	one ideal epidermal patch	655:679	Therefore, it is important to integrate the above properties in one ideal epidermal patch.
33164509	1	63	theme	drug	126:129	arg1	systems					139:145	In vitro drug release systems	117:145	In vitro drug release systems	117:145	In vitro drug release systems have recently received tremendous attention because they allow noninvasive, convenient, and prolonged administration of pharmacological agents.
33164509	11	64	theme	Biological	1779:1788	arg1	experiments					1790:1800	Biological experiments	1779:1800	Biological experiments	1779:1800	Biological experiments proved that the drug-loaded P(NIPAM-AAc) hydrogel-functionalized textiles had good antibacterial properties and wound-healing effects.
33164509	2	65	theme	epidermal	301:309	arg1	systems					324:330	On-demand epidermal drug release systems	291:330	On-demand epidermal drug release systems	291:330	On-demand epidermal drug release systems can improve treatment efficiency, prevent multidrug resistance, and minimize drug toxicity to healthy cells.
33164509	0	66	theme	Visual	63:68	arg1	Monitoring					70:79	Synchronous Visual Monitoring	51:79	Synchronous Visual Monitoring of On-Demand	51:92	Thermochromic Hydrogel-Functionalized Textiles for Synchronous Visual Monitoring of On-Demand In Vitro Drug Release.
33164509	8	67	theme	hydrogel-functionalized	1399:1421	arg1	textiles					1423:1430	P(NIPAM-AAc) hydrogel-functionalized textiles	1386:1430	P(NIPAM-AAc) hydrogel-functionalized textiles	1386:1430	Moreover, the lattice spacing (d) of PCs varied with the expansion and contraction of the hydrogels, which can cause the color of P(NIPAM-AAc) hydrogel-functionalized textiles to change.
33164509	10	68	theme	mechanical	1635:1644	arg1	properties					1646:1655	the poor mechanical properties	1626:1655	the poor mechanical properties of hydrogel wound dressings	1626:1683	Furthermore, in view of the poor mechanical properties of hydrogel wound dressings, textile matrices were composited to prevent holistic breaking during the stretching process.
33164509	0	69	theme	In	94:95	arg1	Release					108:114	In Vitro Drug Release	94:114	In Vitro Drug Release	94:114	Thermochromic Hydrogel-Functionalized Textiles for Synchronous Visual Monitoring of On-Demand In Vitro Drug Release.
33164509	8	70	theme	lattice	1270:1276	arg1	spacing					1278:1284	the lattice spacing	1266:1284	the lattice spacing (d) of PCs	1266:1295	Moreover, the lattice spacing (d) of PCs varied with the expansion and contraction of the hydrogels, which can cause the color of P(NIPAM-AAc) hydrogel-functionalized textiles to change.
33164509	8	70	theme	lattice	1270:1276	arg1	d					1287:1287	d	1287:1287	d	1287:1287	Moreover, the lattice spacing (d) of PCs varied with the expansion and contraction of the hydrogels, which can cause the color of P(NIPAM-AAc) hydrogel-functionalized textiles to change.
33164509	0	71	theme	Drug	103:106	arg1	Release					108:114	In Vitro Drug Release	94:114	In Vitro Drug Release	94:114	Thermochromic Hydrogel-Functionalized Textiles for Synchronous Visual Monitoring of On-Demand In Vitro Drug Release.
33164509	3	72	from	addition	444:451	arg1	essential					499:507	essential	499:507	essential	499:507	In addition, real-time monitoring of drug content is also essential for guiding the determination of drug dosage and replacing drug carriers in time.
33164509	11	73	theme	NIPAM-AAc	1832:1840	arg1	textiles					1867:1874	the drug-loaded P(NIPAM-AAc) hydrogel-functionalized textiles	1814:1874	the drug-loaded P(NIPAM-AAc) hydrogel-functionalized textiles	1814:1874	Biological experiments proved that the drug-loaded P(NIPAM-AAc) hydrogel-functionalized textiles had good antibacterial properties and wound-healing effects.
33164509	1	74	theme	tremendous	170:179	arg1	attention					181:189	tremendous attention	170:189	tremendous attention	170:189	In vitro drug release systems have recently received tremendous attention because they allow noninvasive, convenient, and prolonged administration of pharmacological agents.
33164509	5	75	theme	C	729:729	arg1	nanoparticles					731:743	Fe3O4@C nanoparticles	723:743	Fe3O4@C nanoparticles	723:743	Herein, photonic crystals (PCs) based on Fe3O4@C nanoparticles were introduced into drug-loaded poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAM-AAc)) hydrogel-functionalized textiles.
33164509	9	76	theme	drug	1486:1489	arg1	uptake					1491:1496	thermoresponsive chromic drug uptake	1461:1496	thermoresponsive chromic drug uptake	1461:1496	These synchronous thermoresponsive chromic drug uptake and release behaviors provided an effective method for visual and real-time monitoring of drug content.
33164509	10	77	theme	poor	1630:1633	arg1	properties					1646:1655	the poor mechanical properties	1626:1655	the poor mechanical properties of hydrogel wound dressings	1626:1683	Furthermore, in view of the poor mechanical properties of hydrogel wound dressings, textile matrices were composited to prevent holistic breaking during the stretching process.
33164509	11	78	contain	had	1876:1878	arg2	effects					1928:1934	wound-healing effects	1914:1934	wound-healing effects	1914:1934	Biological experiments proved that the drug-loaded P(NIPAM-AAc) hydrogel-functionalized textiles had good antibacterial properties and wound-healing effects.
33164509	11	78	contain	had	1876:1878	arg1	textiles					1867:1874	the drug-loaded P(NIPAM-AAc) hydrogel-functionalized textiles	1814:1874	the drug-loaded P(NIPAM-AAc) hydrogel-functionalized textiles	1814:1874	Biological experiments proved that the drug-loaded P(NIPAM-AAc) hydrogel-functionalized textiles had good antibacterial properties and wound-healing effects.
33164509	11	78	contain	had	1876:1878	arg2	properties					1899:1908	good antibacterial properties	1880:1908	good antibacterial properties	1880:1908	Biological experiments proved that the drug-loaded P(NIPAM-AAc) hydrogel-functionalized textiles had good antibacterial properties and wound-healing effects.
33164509	7	79	theme	solution	975:982	arg1	LCST					997:1000	LCST	997:1000	LCST	997:1000	The lower critical solution temperature (LCST) of the hydrogels was adjusted to 40 °C, which is higher than the skin temperature, by varying the content of hydrophilic comonomer acrylic acid (AAc) to store the drug at room temperature, and on-demand release was achieved by mild thermal stimulation.
33164509	7	79	theme	solution	975:982	arg1	temperature					984:994	The lower critical solution temperature	956:994	The lower critical solution temperature (LCST) of the hydrogels	956:1018	The lower critical solution temperature (LCST) of the hydrogels was adjusted to 40 °C, which is higher than the skin temperature, by varying the content of hydrophilic comonomer acrylic acid (AAc) to store the drug at room temperature, and on-demand release was achieved by mild thermal stimulation.
33164509	8	80	theme	PCs	1293:1295	arg1	spacing					1278:1284	the lattice spacing	1266:1284	the lattice spacing (d) of PCs	1266:1295	Moreover, the lattice spacing (d) of PCs varied with the expansion and contraction of the hydrogels, which can cause the color of P(NIPAM-AAc) hydrogel-functionalized textiles to change.
33164509	8	80	theme	PCs	1293:1295	arg1	d					1287:1287	d	1287:1287	d	1287:1287	Moreover, the lattice spacing (d) of PCs varied with the expansion and contraction of the hydrogels, which can cause the color of P(NIPAM-AAc) hydrogel-functionalized textiles to change.
33164509	3	81	theme	content	483:489	arg1	monitoring					464:473	real-time monitoring	454:473	real-time monitoring of drug content	454:489	In addition, real-time monitoring of drug content is also essential for guiding the determination of drug dosage and replacing drug carriers in time.
33164509	0	82	theme	Hydrogel-Functionalized	14:36	arg1	Textiles					38:45	Thermochromic Hydrogel-Functionalized Textiles	0:45	Thermochromic Hydrogel-Functionalized Textiles for Synchronous Visual Monitoring of On-Demand	0:92	Thermochromic Hydrogel-Functionalized Textiles for Synchronous Visual Monitoring of On-Demand In Vitro Drug Release.
33164509	7	83	theme	lower	960:964	arg1	LCST					997:1000	LCST	997:1000	LCST	997:1000	The lower critical solution temperature (LCST) of the hydrogels was adjusted to 40 °C, which is higher than the skin temperature, by varying the content of hydrophilic comonomer acrylic acid (AAc) to store the drug at room temperature, and on-demand release was achieved by mild thermal stimulation.
33164509	7	83	theme	lower	960:964	arg1	temperature					984:994	The lower critical solution temperature	956:994	The lower critical solution temperature (LCST) of the hydrogels	956:1018	The lower critical solution temperature (LCST) of the hydrogels was adjusted to 40 °C, which is higher than the skin temperature, by varying the content of hydrophilic comonomer acrylic acid (AAc) to store the drug at room temperature, and on-demand release was achieved by mild thermal stimulation.
33164509	0	84	dep	In	94:95	arg1	Vitro					97:101	Vitro	97:101	Vitro	97:101	Thermochromic Hydrogel-Functionalized Textiles for Synchronous Visual Monitoring of On-Demand In Vitro Drug Release.
33164509	5	85	theme	N-isopropylacrylamide-co-acrylic	783:814	arg1	poly					778:781	drug-loaded poly	766:781	drug-loaded poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAM-AAc)) hydrogel-functionalized textiles	766:868	Herein, photonic crystals (PCs) based on Fe3O4@C nanoparticles were introduced into drug-loaded poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAM-AAc)) hydrogel-functionalized textiles.
33164509	5	85	theme	N-isopropylacrylamide-co-acrylic	783:814	arg1	acid					816:819	N-isopropylacrylamide-co-acrylic acid	783:819	N-isopropylacrylamide-co-acrylic acid	783:819	Herein, photonic crystals (PCs) based on Fe3O4@C nanoparticles were introduced into drug-loaded poly(N-isopropylacrylamide-co-acrylic acid) (P(NIPAM-AAc)) hydrogel-functionalized textiles.
33164509	3	86	theme	real-time	454:462	arg1	monitoring					464:473	real-time monitoring	454:473	real-time monitoring of drug content	454:489	In addition, real-time monitoring of drug content is also essential for guiding the determination of drug dosage and replacing drug carriers in time.
33164509	7	87	theme	hydrophilic	1112:1122	arg1	AAc					1148:1150	AAc	1148:1150	AAc	1148:1150	The lower critical solution temperature (LCST) of the hydrogels was adjusted to 40 °C, which is higher than the skin temperature, by varying the content of hydrophilic comonomer acrylic acid (AAc) to store the drug at room temperature, and on-demand release was achieved by mild thermal stimulation.
33164509	7	87	theme	hydrophilic	1112:1122	arg1	acid					1142:1145	hydrophilic comonomer acrylic acid	1112:1145	hydrophilic comonomer acrylic acid (AAc)	1112:1151	The lower critical solution temperature (LCST) of the hydrogels was adjusted to 40 °C, which is higher than the skin temperature, by varying the content of hydrophilic comonomer acrylic acid (AAc) to store the drug at room temperature, and on-demand release was achieved by mild thermal stimulation.
33164509	9	88	theme	visual	1553:1558	arg1	monitoring					1574:1583	visual and real-time monitoring	1553:1583	visual and real-time monitoring of drug content	1553:1599	These synchronous thermoresponsive chromic drug uptake and release behaviors provided an effective method for visual and real-time monitoring of drug content.
33164509	7	89	theme	thermal	1235:1241	arg1	stimulation					1243:1253	mild thermal stimulation	1230:1253	mild thermal stimulation	1230:1253	The lower critical solution temperature (LCST) of the hydrogels was adjusted to 40 °C, which is higher than the skin temperature, by varying the content of hydrophilic comonomer acrylic acid (AAc) to store the drug at room temperature, and on-demand release was achieved by mild thermal stimulation.
33164509	3	90	from	essential	499:507	arg1	addition					444:451	addition	444:451	addition	444:451	In addition, real-time monitoring of drug content is also essential for guiding the determination of drug dosage and replacing drug carriers in time.
33164509	10	91	theme	properties	1646:1655	arg1	view					1618:1621	view	1618:1621	view of the poor mechanical properties of hydrogel wound dressings	1618:1683	Furthermore, in view of the poor mechanical properties of hydrogel wound dressings, textile matrices were composited to prevent holistic breaking during the stretching process.
33164509	9	92	theme	real-time	1564:1572	arg1	monitoring					1574:1583	visual and real-time monitoring	1553:1583	visual and real-time monitoring of drug content	1553:1599	These synchronous thermoresponsive chromic drug uptake and release behaviors provided an effective method for visual and real-time monitoring of drug content.
33164509	4	93	theme	above	635:639	arg1	properties					641:650	the above properties	631:650	the above properties in one ideal epidermal patch	631:679	Therefore, it is important to integrate the above properties in one ideal epidermal patch.
33164509	9	94	theme	release	1502:1508	arg1	behaviors					1510:1518	These synchronous thermoresponsive chromic drug uptake and release behaviors	1443:1518	These synchronous thermoresponsive chromic drug uptake and release behaviors	1443:1518	These synchronous thermoresponsive chromic drug uptake and release behaviors provided an effective method for visual and real-time monitoring of drug content.
33164509	2	95	theme	drug	409:412	arg1	toxicity					414:421	drug toxicity	409:421	drug toxicity to healthy cells	409:438	On-demand epidermal drug release systems can improve treatment efficiency, prevent multidrug resistance, and minimize drug toxicity to healthy cells.
33164509	11	96	theme	antibacterial	1885:1897	arg1	properties					1899:1908	good antibacterial properties	1880:1908	good antibacterial properties	1880:1908	Biological experiments proved that the drug-loaded P(NIPAM-AAc) hydrogel-functionalized textiles had good antibacterial properties and wound-healing effects.
33164509	6	97	dep	expansion	912:920	arg1	the					908:910	the	908:910	the	908:910	Drug loading and release depended on the expansion and contraction of the hydrogels.
33164509	10	98	theme	dressings	1675:1683	arg1	properties					1646:1655	the poor mechanical properties	1626:1655	the poor mechanical properties of hydrogel wound dressings	1626:1683	Furthermore, in view of the poor mechanical properties of hydrogel wound dressings, textile matrices were composited to prevent holistic breaking during the stretching process.
33164509	1	99	theme	pharmacological	267:281	arg1	agents					283:288	pharmacological agents	267:288	pharmacological agents	267:288	In vitro drug release systems have recently received tremendous attention because they allow noninvasive, convenient, and prolonged administration of pharmacological agents.
32208326	2	0	theme	wet	637:639	arg1	method					654:659	simple and convenient wet ball-milling method	615:659	simple and convenient wet ball-milling method	615:659	The OMMT was synthesized through the intercalation modification of sodium montmorillonite (Na-MMT) with cationic cetyltrimethylammonium bromide (CTAB), nonionic nonylphenol polyoxyethylene ether (NPE) and the mixture of them via simple and convenient wet ball-milling method.
32208326	1	1	with	loading	261:267	arg1	use					335:337	the combined use	322:337	the combined use of organo-montmorillonite (OMMT) and alginate	322:383	The research goal of the present study was to develop a carrier for loading and controlled -release of the hydrophobic drug with the combined use of organo-montmorillonite (OMMT) and alginate.
32208326	4	2	theme	transmission	1086:1097	arg1	microscopy					1108:1117	transmission electron microscopy	1086:1117	transmission electron microscopy (TEM)	1086:1123	The physical and chemical properties of OMMT were comparatively evaluated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analyzer (TGA), BET-specific surface area measurements, and drug adsorption experiments.
32208326	4	2	theme	transmission	1086:1097	arg1	TEM					1120:1122	TEM	1120:1122	TEM	1120:1122	The physical and chemical properties of OMMT were comparatively evaluated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analyzer (TGA), BET-specific surface area measurements, and drug adsorption experiments.
32208326	10	3	theme	drug	2242:2245	arg1	properties					2255:2264	excellent controlled drug release properties	2221:2264	excellent controlled drug release properties	2221:2264	Based on the high drug loading capacity and excellent controlled drug release properties, the CTA/NPE-MMT/Alg incorporating hydrophobic drugs into hydrophilic matrices could be a highly promising material for use in hydrophobic drug delivery.
32208326	1	4	theme	hydrophobic	300:310	arg1	drug					312:315	the hydrophobic drug	296:315	the hydrophobic drug	296:315	The research goal of the present study was to develop a carrier for loading and controlled -release of the hydrophobic drug with the combined use of organo-montmorillonite (OMMT) and alginate.
32208326	4	5	theme	physical	903:910	arg1	properties					925:934	The physical and chemical properties	899:934	The physical and chemical properties of OMMT	899:942	The physical and chemical properties of OMMT were comparatively evaluated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analyzer (TGA), BET-specific surface area measurements, and drug adsorption experiments.
32208326	3	6	theme	controlled	761:770	arg1	properties					780:789	slow and controlled release properties	752:789	slow and controlled release properties	752:789	Furthermore, the organo-montmorillonite/alginate (OMMT/Alg) composite hydrogel beads with slow and controlled release properties were constructed by using alginate as a coating material under the exogenous cross-linking of calcium ions.
32208326	8	7	theme	highest	1928:1934	arg1	LC					1954:1955	LC	1954:1955	LC	1954:1955	In particular, CTA/NPE-MMT/Alg with the highest loading capacity (LC) and encapsulation efficiency (EE) revealed the optimal controlled performance for the release of hydrophobic ibuprofen.
32208326	8	7	theme	highest	1928:1934	arg1	capacity					1944:1951	the highest loading capacity	1924:1951	the highest loading capacity (LC)	1924:1956	In particular, CTA/NPE-MMT/Alg with the highest loading capacity (LC) and encapsulation efficiency (EE) revealed the optimal controlled performance for the release of hydrophobic ibuprofen.
32208326	1	8	theme	present	218:224	arg1	study					226:230	the present study	214:230	the present study	214:230	The research goal of the present study was to develop a carrier for loading and controlled -release of the hydrophobic drug with the combined use of organo-montmorillonite (OMMT) and alginate.
32208326	4	9	theme	chemical	916:923	arg1	properties					925:934	The physical and chemical properties	899:934	The physical and chemical properties of OMMT	899:942	The physical and chemical properties of OMMT were comparatively evaluated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analyzer (TGA), BET-specific surface area measurements, and drug adsorption experiments.
32208326	4	10	theme	thermogravimetric	1158:1174	arg1	TGA					1186:1188	TGA	1186:1188	TGA	1186:1188	The physical and chemical properties of OMMT were comparatively evaluated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analyzer (TGA), BET-specific surface area measurements, and drug adsorption experiments.
32208326	4	10	theme	thermogravimetric	1158:1174	arg1	analyzer					1176:1183	thermogravimetric analyzer	1158:1183	thermogravimetric analyzer (TGA)	1158:1189	The physical and chemical properties of OMMT were comparatively evaluated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analyzer (TGA), BET-specific surface area measurements, and drug adsorption experiments.
32208326	2	11	mod	modification	437:448	arg3	intercalation					423:435	the intercalation modification	419:448	the intercalation modification of sodium montmorillonite (Na-MMT)	419:483	The OMMT was synthesized through the intercalation modification of sodium montmorillonite (Na-MMT) with cationic cetyltrimethylammonium bromide (CTAB), nonionic nonylphenol polyoxyethylene ether (NPE) and the mixture of them via simple and convenient wet ball-milling method.
32208326	2	11	mod	modification	437:448	arg1	Na-MMT					477:482	Na-MMT	477:482	Na-MMT	477:482	The OMMT was synthesized through the intercalation modification of sodium montmorillonite (Na-MMT) with cationic cetyltrimethylammonium bromide (CTAB), nonionic nonylphenol polyoxyethylene ether (NPE) and the mixture of them via simple and convenient wet ball-milling method.
32208326	2	11	mod	modification	437:448	arg1	montmorillonite					460:474	sodium montmorillonite	453:474	sodium montmorillonite (Na-MMT)	453:483	The OMMT was synthesized through the intercalation modification of sodium montmorillonite (Na-MMT) with cationic cetyltrimethylammonium bromide (CTAB), nonionic nonylphenol polyoxyethylene ether (NPE) and the mixture of them via simple and convenient wet ball-milling method.
32208326	10	12	theme	promising	2363:2371	arg1	material					2373:2380	a highly promising material	2354:2380	a highly promising material for use in hydrophobic drug delivery	2354:2417	Based on the high drug loading capacity and excellent controlled drug release properties, the CTA/NPE-MMT/Alg incorporating hydrophobic drugs into hydrophilic matrices could be a highly promising material for use in hydrophobic drug delivery.
32208326	10	12	theme	promising	2363:2371	arg1	CTA/NPE-MMT/Alg					2271:2285	the CTA/NPE-MMT/Alg	2267:2285	the CTA/NPE-MMT/Alg incorporating hydrophobic drugs into hydrophilic matrices	2267:2343	Based on the high drug loading capacity and excellent controlled drug release properties, the CTA/NPE-MMT/Alg incorporating hydrophobic drugs into hydrophilic matrices could be a highly promising material for use in hydrophobic drug delivery.
32208326	7	13	theme	good	1812:1815	arg1	affinity					1817:1824	the good affinity	1808:1824	the good affinity of OMMT to hydrophobic drug that retarded the drug diffusion	1808:1885	In addition, the OMMT/Alg composite hydrogel beads displayed superior sustained-release properties than Na-MMT/Alg, mainly ascribed to the good affinity of OMMT to hydrophobic drug that retarded the drug diffusion.
32208326	4	14	theme	light	1134:1138	arg1	DLS					1152:1154	DLS	1152:1154	DLS	1152:1154	The physical and chemical properties of OMMT were comparatively evaluated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analyzer (TGA), BET-specific surface area measurements, and drug adsorption experiments.
32208326	4	14	theme	light	1134:1138	arg1	scattering					1140:1149	dynamic light scattering	1126:1149	dynamic light scattering (DLS)	1126:1155	The physical and chemical properties of OMMT were comparatively evaluated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analyzer (TGA), BET-specific surface area measurements, and drug adsorption experiments.
32208326	0	15	theme	pharmaceutical	146:159	arg1	formulation					180:190	organo-montmorillonite/alginate based hydrophobic pharmaceutical controlled-release formulation	96:190	organo-montmorillonite/alginate based hydrophobic pharmaceutical controlled-release formulation	96:190	Synthesis and assessment of CTAB and NPE modified organo-montmorillonite for the fabrication of organo-montmorillonite/alginate based hydrophobic pharmaceutical controlled-release formulation.
32208326	6	16	theme	ball-milling	1539:1550	arg1	process					1552:1558	the wet ball-milling process	1531:1558	the wet ball-milling process	1531:1558	And the cationic CTAB and nonionic NPE were adsorbed or intercalated into the MMT lamellar structure through the wet ball-milling process, which could change the hydrophilic nature of Na-MMT and improve its affinity to the hydrophobic drug molecules.
32208326	10	17	theme	drug	2195:2198	arg1	capacity					2208:2215	the high drug loading capacity	2186:2215	the high drug loading capacity	2186:2215	Based on the high drug loading capacity and excellent controlled drug release properties, the CTA/NPE-MMT/Alg incorporating hydrophobic drugs into hydrophilic matrices could be a highly promising material for use in hydrophobic drug delivery.
32208326	3	18	theme	coating	831:837	arg1	alginate					817:824	alginate	817:824	alginate	817:824	Furthermore, the organo-montmorillonite/alginate (OMMT/Alg) composite hydrogel beads with slow and controlled release properties were constructed by using alginate as a coating material under the exogenous cross-linking of calcium ions.
32208326	3	18	theme	coating	831:837	arg1	material					839:846	a coating material	829:846	a coating material under the exogenous cross-linking of calcium ions	829:896	Furthermore, the organo-montmorillonite/alginate (OMMT/Alg) composite hydrogel beads with slow and controlled release properties were constructed by using alginate as a coating material under the exogenous cross-linking of calcium ions.
32208326	0	19	theme	formulation	180:190	arg1	fabrication					81:91	the fabrication	77:91	the fabrication of organo-montmorillonite/alginate based hydrophobic pharmaceutical controlled-release formulation	77:190	Synthesis and assessment of CTAB and NPE modified organo-montmorillonite for the fabrication of organo-montmorillonite/alginate based hydrophobic pharmaceutical controlled-release formulation.
32208326	4	20	theme	BET-specific	1192:1203	arg1	measurements					1218:1229	BET-specific surface area measurements	1192:1229	BET-specific surface area measurements	1192:1229	The physical and chemical properties of OMMT were comparatively evaluated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analyzer (TGA), BET-specific surface area measurements, and drug adsorption experiments.
32208326	10	21	theme	hydrophilic	2324:2334	arg1	matrices					2336:2343	hydrophilic matrices	2324:2343	hydrophilic matrices	2324:2343	Based on the high drug loading capacity and excellent controlled drug release properties, the CTA/NPE-MMT/Alg incorporating hydrophobic drugs into hydrophilic matrices could be a highly promising material for use in hydrophobic drug delivery.
32208326	4	22	theme	scanning	976:983	arg1	microscopy					994:1003	scanning electron microscopy	976:1003	scanning electron microscopy (SEM)	976:1009	The physical and chemical properties of OMMT were comparatively evaluated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analyzer (TGA), BET-specific surface area measurements, and drug adsorption experiments.
32208326	4	22	theme	scanning	976:983	arg1	SEM					1006:1008	SEM	1006:1008	SEM	1006:1008	The physical and chemical properties of OMMT were comparatively evaluated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analyzer (TGA), BET-specific surface area measurements, and drug adsorption experiments.
32208326	4	23	theme	area	1213:1216	arg1	measurements					1218:1229	BET-specific surface area measurements	1192:1229	BET-specific surface area measurements	1192:1229	The physical and chemical properties of OMMT were comparatively evaluated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analyzer (TGA), BET-specific surface area measurements, and drug adsorption experiments.
32208326	5	24	theme	CTAB	1314:1317	arg1	presence					1302:1309	the presence	1298:1309	the presence of CTAB	1298:1317	Experimental results showed that the presence of CTAB was able to facilitate the intercalation of CTAB/NPE into Na-MMT through the cation exchange reaction.
32208326	5	24	theme	CTAB	1314:1317	arg1	able					1323:1326	able	1323:1326	able	1323:1326	Experimental results showed that the presence of CTAB was able to facilitate the intercalation of CTAB/NPE into Na-MMT through the cation exchange reaction.
32208326	3	25	theme	exogenous	858:866	arg1	cross-linking					868:880	the exogenous cross-linking	854:880	the exogenous cross-linking of calcium ions	854:896	Furthermore, the organo-montmorillonite/alginate (OMMT/Alg) composite hydrogel beads with slow and controlled release properties were constructed by using alginate as a coating material under the exogenous cross-linking of calcium ions.
32208326	10	26	theme	excellent	2221:2229	arg1	properties					2255:2264	excellent controlled drug release properties	2221:2264	excellent controlled drug release properties	2221:2264	Based on the high drug loading capacity and excellent controlled drug release properties, the CTA/NPE-MMT/Alg incorporating hydrophobic drugs into hydrophilic matrices could be a highly promising material for use in hydrophobic drug delivery.
32208326	6	27	theme	Na-MMT	1606:1611	arg1	nature					1596:1601	the hydrophilic nature	1580:1601	the hydrophilic nature of Na-MMT	1580:1611	And the cationic CTAB and nonionic NPE were adsorbed or intercalated into the MMT lamellar structure through the wet ball-milling process, which could change the hydrophilic nature of Na-MMT and improve its affinity to the hydrophobic drug molecules.
32208326	2	28	theme	nonionic	538:545	arg1	NPE					582:584	NPE	582:584	NPE	582:584	The OMMT was synthesized through the intercalation modification of sodium montmorillonite (Na-MMT) with cationic cetyltrimethylammonium bromide (CTAB), nonionic nonylphenol polyoxyethylene ether (NPE) and the mixture of them via simple and convenient wet ball-milling method.
32208326	2	28	theme	nonionic	538:545	arg1	ether					575:579	nonionic nonylphenol polyoxyethylene ether	538:579	nonionic nonylphenol polyoxyethylene ether (NPE)	538:585	The OMMT was synthesized through the intercalation modification of sodium montmorillonite (Na-MMT) with cationic cetyltrimethylammonium bromide (CTAB), nonionic nonylphenol polyoxyethylene ether (NPE) and the mixture of them via simple and convenient wet ball-milling method.
32208326	1	29	theme	research	197:204	arg1	goal					206:209	The research goal	193:209	The research goal of the present study	193:230	The research goal of the present study was to develop a carrier for loading and controlled -release of the hydrophobic drug with the combined use of organo-montmorillonite (OMMT) and alginate.
32208326	3	30	theme	ions	893:896	arg1	cross-linking					868:880	the exogenous cross-linking	854:880	the exogenous cross-linking of calcium ions	854:896	Furthermore, the organo-montmorillonite/alginate (OMMT/Alg) composite hydrogel beads with slow and controlled release properties were constructed by using alginate as a coating material under the exogenous cross-linking of calcium ions.
32208326	8	31	theme	optimal	2005:2011	arg1	performance					2024:2034	the optimal controlled performance	2001:2034	the optimal controlled performance for the release of hydrophobic ibuprofen	2001:2075	In particular, CTA/NPE-MMT/Alg with the highest loading capacity (LC) and encapsulation efficiency (EE) revealed the optimal controlled performance for the release of hydrophobic ibuprofen.
32208326	2	32	theme	simple	615:620	arg1	method					654:659	simple and convenient wet ball-milling method	615:659	simple and convenient wet ball-milling method	615:659	The OMMT was synthesized through the intercalation modification of sodium montmorillonite (Na-MMT) with cationic cetyltrimethylammonium bromide (CTAB), nonionic nonylphenol polyoxyethylene ether (NPE) and the mixture of them via simple and convenient wet ball-milling method.
32208326	7	33	theme	sustained-release	1743:1759	arg1	properties					1761:1770	superior sustained-release properties	1734:1770	superior sustained-release properties	1734:1770	In addition, the OMMT/Alg composite hydrogel beads displayed superior sustained-release properties than Na-MMT/Alg, mainly ascribed to the good affinity of OMMT to hydrophobic drug that retarded the drug diffusion.
32208326	9	34	theme	Korsmeyer-Peppas	2103:2118	arg1	model					2120:2124	the Korsmeyer-Peppas model	2099:2124	the Korsmeyer-Peppas model suggested non-Fickian diffusion release mechanism	2099:2174	The release followed the Korsmeyer-Peppas model suggested non-Fickian diffusion release mechanism.
32208326	2	35	theme	polyoxyethylene	559:573	arg1	NPE					582:584	NPE	582:584	NPE	582:584	The OMMT was synthesized through the intercalation modification of sodium montmorillonite (Na-MMT) with cationic cetyltrimethylammonium bromide (CTAB), nonionic nonylphenol polyoxyethylene ether (NPE) and the mixture of them via simple and convenient wet ball-milling method.
32208326	2	35	theme	polyoxyethylene	559:573	arg1	ether					575:579	nonionic nonylphenol polyoxyethylene ether	538:579	nonionic nonylphenol polyoxyethylene ether (NPE)	538:585	The OMMT was synthesized through the intercalation modification of sodium montmorillonite (Na-MMT) with cationic cetyltrimethylammonium bromide (CTAB), nonionic nonylphenol polyoxyethylene ether (NPE) and the mixture of them via simple and convenient wet ball-milling method.
32208326	5	36	theme	CTAB/NPE	1363:1370	arg1	intercalation					1346:1358	the intercalation	1342:1358	the intercalation of CTAB/NPE into Na-MMT	1342:1382	Experimental results showed that the presence of CTAB was able to facilitate the intercalation of CTAB/NPE into Na-MMT through the cation exchange reaction.
32208326	2	37	theme	intercalation	423:435	arg1	modification					437:448	the intercalation modification	419:448	the intercalation modification of sodium montmorillonite (Na-MMT)	419:483	The OMMT was synthesized through the intercalation modification of sodium montmorillonite (Na-MMT) with cationic cetyltrimethylammonium bromide (CTAB), nonionic nonylphenol polyoxyethylene ether (NPE) and the mixture of them via simple and convenient wet ball-milling method.
32208326	0	38	theme	based	128:132	arg1	formulation					180:190	organo-montmorillonite/alginate based hydrophobic pharmaceutical controlled-release formulation	96:190	organo-montmorillonite/alginate based hydrophobic pharmaceutical controlled-release formulation	96:190	Synthesis and assessment of CTAB and NPE modified organo-montmorillonite for the fabrication of organo-montmorillonite/alginate based hydrophobic pharmaceutical controlled-release formulation.
32208326	6	39	theme	drug	1657:1660	arg1	molecules					1662:1670	the hydrophobic drug molecules	1641:1670	the hydrophobic drug molecules	1641:1670	And the cationic CTAB and nonionic NPE were adsorbed or intercalated into the MMT lamellar structure through the wet ball-milling process, which could change the hydrophilic nature of Na-MMT and improve its affinity to the hydrophobic drug molecules.
32208326	7	40	theme	hydrogel	1709:1716	arg1	beads					1718:1722	the OMMT/Alg composite hydrogel beads	1686:1722	the OMMT/Alg composite hydrogel beads	1686:1722	In addition, the OMMT/Alg composite hydrogel beads displayed superior sustained-release properties than Na-MMT/Alg, mainly ascribed to the good affinity of OMMT to hydrophobic drug that retarded the drug diffusion.
32208326	1	41	theme	drug	312:315	arg1	loading					261:267	loading	261:267	loading	261:267	The research goal of the present study was to develop a carrier for loading and controlled -release of the hydrophobic drug with the combined use of organo-montmorillonite (OMMT) and alginate.
32208326	1	41	theme	drug	312:315	arg1	-release					284:291	controlled -release	273:291	controlled -release	273:291	The research goal of the present study was to develop a carrier for loading and controlled -release of the hydrophobic drug with the combined use of organo-montmorillonite (OMMT) and alginate.
32208326	10	42	theme	hydrophobic	2393:2403	arg1	delivery					2410:2417	hydrophobic drug delivery	2393:2417	hydrophobic drug delivery	2393:2417	Based on the high drug loading capacity and excellent controlled drug release properties, the CTA/NPE-MMT/Alg incorporating hydrophobic drugs into hydrophilic matrices could be a highly promising material for use in hydrophobic drug delivery.
32208326	7	43	theme	OMMT/Alg	1690:1697	arg1	beads					1718:1722	the OMMT/Alg composite hydrogel beads	1686:1722	the OMMT/Alg composite hydrogel beads	1686:1722	In addition, the OMMT/Alg composite hydrogel beads displayed superior sustained-release properties than Na-MMT/Alg, mainly ascribed to the good affinity of OMMT to hydrophobic drug that retarded the drug diffusion.
32208326	4	44	theme	drug	1236:1239	arg1	experiments					1252:1262	drug adsorption experiments	1236:1262	drug adsorption experiments	1236:1262	The physical and chemical properties of OMMT were comparatively evaluated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analyzer (TGA), BET-specific surface area measurements, and drug adsorption experiments.
32208326	2	45	theme	montmorillonite	460:474	arg1	modification					437:448	the intercalation modification	419:448	the intercalation modification of sodium montmorillonite (Na-MMT)	419:483	The OMMT was synthesized through the intercalation modification of sodium montmorillonite (Na-MMT) with cationic cetyltrimethylammonium bromide (CTAB), nonionic nonylphenol polyoxyethylene ether (NPE) and the mixture of them via simple and convenient wet ball-milling method.
32208326	8	46	theme	ibuprofen	2067:2075	arg1	release					2044:2050	the release	2040:2050	the release of hydrophobic ibuprofen	2040:2075	In particular, CTA/NPE-MMT/Alg with the highest loading capacity (LC) and encapsulation efficiency (EE) revealed the optimal controlled performance for the release of hydrophobic ibuprofen.
32208326	0	47	theme	CTAB	28:31	arg1	assessment					14:23	assessment	14:23	assessment	14:23	Synthesis and assessment of CTAB and NPE modified organo-montmorillonite for the fabrication of organo-montmorillonite/alginate based hydrophobic pharmaceutical controlled-release formulation.
32208326	0	47	theme	CTAB	28:31	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and assessment of CTAB and NPE modified organo-montmorillonite for the fabrication of organo-montmorillonite/alginate based hydrophobic pharmaceutical controlled-release formulation.
32208326	2	48	theme	cetyltrimethylammonium	499:520	arg1	CTAB					531:534	CTAB	531:534	CTAB	531:534	The OMMT was synthesized through the intercalation modification of sodium montmorillonite (Na-MMT) with cationic cetyltrimethylammonium bromide (CTAB), nonionic nonylphenol polyoxyethylene ether (NPE) and the mixture of them via simple and convenient wet ball-milling method.
32208326	2	48	theme	cetyltrimethylammonium	499:520	arg1	bromide					522:528	cationic cetyltrimethylammonium bromide	490:528	cationic cetyltrimethylammonium bromide (CTAB)	490:535	The OMMT was synthesized through the intercalation modification of sodium montmorillonite (Na-MMT) with cationic cetyltrimethylammonium bromide (CTAB), nonionic nonylphenol polyoxyethylene ether (NPE) and the mixture of them via simple and convenient wet ball-milling method.
32208326	9	49	theme	non-Fickian	2136:2146	arg1	mechanism					2166:2174	non-Fickian diffusion release mechanism	2136:2174	non-Fickian diffusion release mechanism	2136:2174	The release followed the Korsmeyer-Peppas model suggested non-Fickian diffusion release mechanism.
32208326	0	50	theme	NPE	37:39	arg1	assessment					14:23	assessment	14:23	assessment	14:23	Synthesis and assessment of CTAB and NPE modified organo-montmorillonite for the fabrication of organo-montmorillonite/alginate based hydrophobic pharmaceutical controlled-release formulation.
32208326	0	50	theme	NPE	37:39	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and assessment of CTAB and NPE modified organo-montmorillonite for the fabrication of organo-montmorillonite/alginate based hydrophobic pharmaceutical controlled-release formulation.
32208326	1	51	theme	organo-montmorillonite	342:363	arg1	use					335:337	the combined use	322:337	the combined use of organo-montmorillonite (OMMT) and alginate	322:383	The research goal of the present study was to develop a carrier for loading and controlled -release of the hydrophobic drug with the combined use of organo-montmorillonite (OMMT) and alginate.
32208326	1	52	with	-release	284:291	arg1	use					335:337	the combined use	322:337	the combined use of organo-montmorillonite (OMMT) and alginate	322:383	The research goal of the present study was to develop a carrier for loading and controlled -release of the hydrophobic drug with the combined use of organo-montmorillonite (OMMT) and alginate.
32208326	3	53	theme	hydrogel	732:739	arg1	beads					741:745	the organo-montmorillonite/alginate (OMMT/Alg) composite hydrogel beads	675:745	the organo-montmorillonite/alginate (OMMT/Alg) composite hydrogel beads with slow and controlled release properties	675:789	Furthermore, the organo-montmorillonite/alginate (OMMT/Alg) composite hydrogel beads with slow and controlled release properties were constructed by using alginate as a coating material under the exogenous cross-linking of calcium ions.
32208326	4	54	theme	dynamic	1126:1132	arg1	DLS					1152:1154	DLS	1152:1154	DLS	1152:1154	The physical and chemical properties of OMMT were comparatively evaluated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analyzer (TGA), BET-specific surface area measurements, and drug adsorption experiments.
32208326	4	54	theme	dynamic	1126:1132	arg1	scattering					1140:1149	dynamic light scattering	1126:1149	dynamic light scattering (DLS)	1126:1155	The physical and chemical properties of OMMT were comparatively evaluated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analyzer (TGA), BET-specific surface area measurements, and drug adsorption experiments.
32208326	6	55	theme	lamellar	1504:1511	arg1	structure					1513:1521	the MMT lamellar structure	1496:1521	the MMT lamellar structure	1496:1521	And the cationic CTAB and nonionic NPE were adsorbed or intercalated into the MMT lamellar structure through the wet ball-milling process, which could change the hydrophilic nature of Na-MMT and improve its affinity to the hydrophobic drug molecules.
32208326	5	56	theme	Experimental	1265:1276	arg1	results					1278:1284	Experimental results	1265:1284	Experimental results	1265:1284	Experimental results showed that the presence of CTAB was able to facilitate the intercalation of CTAB/NPE into Na-MMT through the cation exchange reaction.
32208326	4	57	theme	electron	1099:1106	arg1	microscopy					1108:1117	transmission electron microscopy	1086:1117	transmission electron microscopy (TEM)	1086:1123	The physical and chemical properties of OMMT were comparatively evaluated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analyzer (TGA), BET-specific surface area measurements, and drug adsorption experiments.
32208326	4	57	theme	electron	1099:1106	arg1	TEM					1120:1122	TEM	1120:1122	TEM	1120:1122	The physical and chemical properties of OMMT were comparatively evaluated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analyzer (TGA), BET-specific surface area measurements, and drug adsorption experiments.
32208326	5	58	theme	exchange	1403:1410	arg1	reaction					1412:1419	the cation exchange reaction	1392:1419	the cation exchange reaction	1392:1419	Experimental results showed that the presence of CTAB was able to facilitate the intercalation of CTAB/NPE into Na-MMT through the cation exchange reaction.
32208326	2	59	theme	convenient	626:635	arg1	method					654:659	simple and convenient wet ball-milling method	615:659	simple and convenient wet ball-milling method	615:659	The OMMT was synthesized through the intercalation modification of sodium montmorillonite (Na-MMT) with cationic cetyltrimethylammonium bromide (CTAB), nonionic nonylphenol polyoxyethylene ether (NPE) and the mixture of them via simple and convenient wet ball-milling method.
32208326	7	60	theme	hydrophobic	1837:1847	arg1	drug					1849:1852	hydrophobic drug	1837:1852	hydrophobic drug that retarded the drug diffusion	1837:1885	In addition, the OMMT/Alg composite hydrogel beads displayed superior sustained-release properties than Na-MMT/Alg, mainly ascribed to the good affinity of OMMT to hydrophobic drug that retarded the drug diffusion.
32208326	4	61	theme	OMMT	939:942	arg1	properties					925:934	The physical and chemical properties	899:934	The physical and chemical properties of OMMT	899:942	The physical and chemical properties of OMMT were comparatively evaluated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analyzer (TGA), BET-specific surface area measurements, and drug adsorption experiments.
32208326	7	62	theme	OMMT	1829:1832	arg1	affinity					1817:1824	the good affinity	1808:1824	the good affinity of OMMT to hydrophobic drug that retarded the drug diffusion	1808:1885	In addition, the OMMT/Alg composite hydrogel beads displayed superior sustained-release properties than Na-MMT/Alg, mainly ascribed to the good affinity of OMMT to hydrophobic drug that retarded the drug diffusion.
32208326	10	63	theme	high	2190:2193	arg1	capacity					2208:2215	the high drug loading capacity	2186:2215	the high drug loading capacity	2186:2215	Based on the high drug loading capacity and excellent controlled drug release properties, the CTA/NPE-MMT/Alg incorporating hydrophobic drugs into hydrophilic matrices could be a highly promising material for use in hydrophobic drug delivery.
32208326	8	64	theme	loading	1936:1942	arg1	LC					1954:1955	LC	1954:1955	LC	1954:1955	In particular, CTA/NPE-MMT/Alg with the highest loading capacity (LC) and encapsulation efficiency (EE) revealed the optimal controlled performance for the release of hydrophobic ibuprofen.
32208326	8	64	theme	loading	1936:1942	arg1	capacity					1944:1951	the highest loading capacity	1924:1951	the highest loading capacity (LC)	1924:1956	In particular, CTA/NPE-MMT/Alg with the highest loading capacity (LC) and encapsulation efficiency (EE) revealed the optimal controlled performance for the release of hydrophobic ibuprofen.
32208326	2	65	theme	cationic	490:497	arg1	CTAB					531:534	CTAB	531:534	CTAB	531:534	The OMMT was synthesized through the intercalation modification of sodium montmorillonite (Na-MMT) with cationic cetyltrimethylammonium bromide (CTAB), nonionic nonylphenol polyoxyethylene ether (NPE) and the mixture of them via simple and convenient wet ball-milling method.
32208326	2	65	theme	cationic	490:497	arg1	bromide					522:528	cationic cetyltrimethylammonium bromide	490:528	cationic cetyltrimethylammonium bromide (CTAB)	490:535	The OMMT was synthesized through the intercalation modification of sodium montmorillonite (Na-MMT) with cationic cetyltrimethylammonium bromide (CTAB), nonionic nonylphenol polyoxyethylene ether (NPE) and the mixture of them via simple and convenient wet ball-milling method.
32208326	3	66	theme	release	772:778	arg1	properties					780:789	slow and controlled release properties	752:789	slow and controlled release properties	752:789	Furthermore, the organo-montmorillonite/alginate (OMMT/Alg) composite hydrogel beads with slow and controlled release properties were constructed by using alginate as a coating material under the exogenous cross-linking of calcium ions.
32208326	1	67	theme	study	226:230	arg1	goal					206:209	The research goal	193:209	The research goal of the present study	193:230	The research goal of the present study was to develop a carrier for loading and controlled -release of the hydrophobic drug with the combined use of organo-montmorillonite (OMMT) and alginate.
32208326	2	68	theme	ball-milling	641:652	arg1	method					654:659	simple and convenient wet ball-milling method	615:659	simple and convenient wet ball-milling method	615:659	The OMMT was synthesized through the intercalation modification of sodium montmorillonite (Na-MMT) with cationic cetyltrimethylammonium bromide (CTAB), nonionic nonylphenol polyoxyethylene ether (NPE) and the mixture of them via simple and convenient wet ball-milling method.
32208326	9	69	theme	diffusion	2148:2156	arg1	mechanism					2166:2174	non-Fickian diffusion release mechanism	2136:2174	non-Fickian diffusion release mechanism	2136:2174	The release followed the Korsmeyer-Peppas model suggested non-Fickian diffusion release mechanism.
32208326	0	70	theme	controlled-release	161:178	arg1	formulation					180:190	organo-montmorillonite/alginate based hydrophobic pharmaceutical controlled-release formulation	96:190	organo-montmorillonite/alginate based hydrophobic pharmaceutical controlled-release formulation	96:190	Synthesis and assessment of CTAB and NPE modified organo-montmorillonite for the fabrication of organo-montmorillonite/alginate based hydrophobic pharmaceutical controlled-release formulation.
32208326	4	71	theme	electron	985:992	arg1	microscopy					994:1003	scanning electron microscopy	976:1003	scanning electron microscopy (SEM)	976:1009	The physical and chemical properties of OMMT were comparatively evaluated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analyzer (TGA), BET-specific surface area measurements, and drug adsorption experiments.
32208326	4	71	theme	electron	985:992	arg1	SEM					1006:1008	SEM	1006:1008	SEM	1006:1008	The physical and chemical properties of OMMT were comparatively evaluated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analyzer (TGA), BET-specific surface area measurements, and drug adsorption experiments.
32208326	10	72	theme	loading	2200:2206	arg1	capacity					2208:2215	the high drug loading capacity	2186:2215	the high drug loading capacity	2186:2215	Based on the high drug loading capacity and excellent controlled drug release properties, the CTA/NPE-MMT/Alg incorporating hydrophobic drugs into hydrophilic matrices could be a highly promising material for use in hydrophobic drug delivery.
32208326	8	73	theme	encapsulation	1962:1974	arg1	EE					1988:1989	EE	1988:1989	EE	1988:1989	In particular, CTA/NPE-MMT/Alg with the highest loading capacity (LC) and encapsulation efficiency (EE) revealed the optimal controlled performance for the release of hydrophobic ibuprofen.
32208326	8	73	theme	encapsulation	1962:1974	arg1	efficiency					1976:1985	encapsulation efficiency	1962:1985	encapsulation efficiency (EE)	1962:1990	In particular, CTA/NPE-MMT/Alg with the highest loading capacity (LC) and encapsulation efficiency (EE) revealed the optimal controlled performance for the release of hydrophobic ibuprofen.
32208326	6	74	theme	wet	1535:1537	arg1	process					1552:1558	the wet ball-milling process	1531:1558	the wet ball-milling process	1531:1558	And the cationic CTAB and nonionic NPE were adsorbed or intercalated into the MMT lamellar structure through the wet ball-milling process, which could change the hydrophilic nature of Na-MMT and improve its affinity to the hydrophobic drug molecules.
32208326	10	75	theme	drug	2405:2408	arg1	delivery					2410:2417	hydrophobic drug delivery	2393:2417	hydrophobic drug delivery	2393:2417	Based on the high drug loading capacity and excellent controlled drug release properties, the CTA/NPE-MMT/Alg incorporating hydrophobic drugs into hydrophilic matrices could be a highly promising material for use in hydrophobic drug delivery.
32208326	4	76	theme	surface	1205:1211	arg1	measurements					1218:1229	BET-specific surface area measurements	1192:1229	BET-specific surface area measurements	1192:1229	The physical and chemical properties of OMMT were comparatively evaluated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analyzer (TGA), BET-specific surface area measurements, and drug adsorption experiments.
32208326	2	77	theme	them	606:609	arg1	ether					575:579	nonionic nonylphenol polyoxyethylene ether	538:579	nonionic nonylphenol polyoxyethylene ether (NPE)	538:585	The OMMT was synthesized through the intercalation modification of sodium montmorillonite (Na-MMT) with cationic cetyltrimethylammonium bromide (CTAB), nonionic nonylphenol polyoxyethylene ether (NPE) and the mixture of them via simple and convenient wet ball-milling method.
32208326	2	77	theme	them	606:609	arg1	CTAB					531:534	CTAB	531:534	CTAB	531:534	The OMMT was synthesized through the intercalation modification of sodium montmorillonite (Na-MMT) with cationic cetyltrimethylammonium bromide (CTAB), nonionic nonylphenol polyoxyethylene ether (NPE) and the mixture of them via simple and convenient wet ball-milling method.
32208326	2	77	theme	them	606:609	arg1	bromide					522:528	cationic cetyltrimethylammonium bromide	490:528	cationic cetyltrimethylammonium bromide (CTAB)	490:535	The OMMT was synthesized through the intercalation modification of sodium montmorillonite (Na-MMT) with cationic cetyltrimethylammonium bromide (CTAB), nonionic nonylphenol polyoxyethylene ether (NPE) and the mixture of them via simple and convenient wet ball-milling method.
32208326	2	77	theme	them	606:609	arg1	NPE					582:584	NPE	582:584	NPE	582:584	The OMMT was synthesized through the intercalation modification of sodium montmorillonite (Na-MMT) with cationic cetyltrimethylammonium bromide (CTAB), nonionic nonylphenol polyoxyethylene ether (NPE) and the mixture of them via simple and convenient wet ball-milling method.
32208326	2	77	theme	them	606:609	arg1	mixture					595:601	the mixture	591:601	the mixture of them	591:609	The OMMT was synthesized through the intercalation modification of sodium montmorillonite (Na-MMT) with cationic cetyltrimethylammonium bromide (CTAB), nonionic nonylphenol polyoxyethylene ether (NPE) and the mixture of them via simple and convenient wet ball-milling method.
32208326	9	78	theme	release	2158:2164	arg1	mechanism					2166:2174	non-Fickian diffusion release mechanism	2136:2174	non-Fickian diffusion release mechanism	2136:2174	The release followed the Korsmeyer-Peppas model suggested non-Fickian diffusion release mechanism.
32208326	10	79	theme	controlled	2231:2240	arg1	properties					2255:2264	excellent controlled drug release properties	2221:2264	excellent controlled drug release properties	2221:2264	Based on the high drug loading capacity and excellent controlled drug release properties, the CTA/NPE-MMT/Alg incorporating hydrophobic drugs into hydrophilic matrices could be a highly promising material for use in hydrophobic drug delivery.
32208326	3	80	with	beads	741:745	arg1	properties					780:789	slow and controlled release properties	752:789	slow and controlled release properties	752:789	Furthermore, the organo-montmorillonite/alginate (OMMT/Alg) composite hydrogel beads with slow and controlled release properties were constructed by using alginate as a coating material under the exogenous cross-linking of calcium ions.
32208326	4	81	theme	infrared	1030:1037	arg1	spectroscopy					1039:1050	infrared spectroscopy	1030:1050	infrared spectroscopy (FT-IR)	1030:1058	The physical and chemical properties of OMMT were comparatively evaluated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analyzer (TGA), BET-specific surface area measurements, and drug adsorption experiments.
32208326	4	81	theme	infrared	1030:1037	arg1	FT-IR					1053:1057	FT-IR	1053:1057	FT-IR	1053:1057	The physical and chemical properties of OMMT were comparatively evaluated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analyzer (TGA), BET-specific surface area measurements, and drug adsorption experiments.
32208326	8	82	theme	controlled	2013:2022	arg1	performance					2024:2034	the optimal controlled performance	2001:2034	the optimal controlled performance for the release of hydrophobic ibuprofen	2001:2075	In particular, CTA/NPE-MMT/Alg with the highest loading capacity (LC) and encapsulation efficiency (EE) revealed the optimal controlled performance for the release of hydrophobic ibuprofen.
32208326	6	83	theme	hydrophilic	1584:1594	arg1	nature					1596:1601	the hydrophilic nature	1580:1601	the hydrophilic nature of Na-MMT	1580:1611	And the cationic CTAB and nonionic NPE were adsorbed or intercalated into the MMT lamellar structure through the wet ball-milling process, which could change the hydrophilic nature of Na-MMT and improve its affinity to the hydrophobic drug molecules.
32208326	0	84	theme	organo-montmorillonite/alginate	96:126	arg1	formulation					180:190	organo-montmorillonite/alginate based hydrophobic pharmaceutical controlled-release formulation	96:190	organo-montmorillonite/alginate based hydrophobic pharmaceutical controlled-release formulation	96:190	Synthesis and assessment of CTAB and NPE modified organo-montmorillonite for the fabrication of organo-montmorillonite/alginate based hydrophobic pharmaceutical controlled-release formulation.
32208326	1	85	theme	alginate	376:383	arg1	use					335:337	the combined use	322:337	the combined use of organo-montmorillonite (OMMT) and alginate	322:383	The research goal of the present study was to develop a carrier for loading and controlled -release of the hydrophobic drug with the combined use of organo-montmorillonite (OMMT) and alginate.
32208326	10	86	theme	hydrophobic	2301:2311	arg1	drugs					2313:2317	hydrophobic drugs	2301:2317	hydrophobic drugs	2301:2317	Based on the high drug loading capacity and excellent controlled drug release properties, the CTA/NPE-MMT/Alg incorporating hydrophobic drugs into hydrophilic matrices could be a highly promising material for use in hydrophobic drug delivery.
32208326	3	87	theme	calcium	885:891	arg1	ions					893:896	calcium ions	885:896	calcium ions	885:896	Furthermore, the organo-montmorillonite/alginate (OMMT/Alg) composite hydrogel beads with slow and controlled release properties were constructed by using alginate as a coating material under the exogenous cross-linking of calcium ions.
32208326	0	88	theme	hydrophobic	134:144	arg1	formulation					180:190	organo-montmorillonite/alginate based hydrophobic pharmaceutical controlled-release formulation	96:190	organo-montmorillonite/alginate based hydrophobic pharmaceutical controlled-release formulation	96:190	Synthesis and assessment of CTAB and NPE modified organo-montmorillonite for the fabrication of organo-montmorillonite/alginate based hydrophobic pharmaceutical controlled-release formulation.
32208326	7	89	theme	superior	1734:1741	arg1	properties					1761:1770	superior sustained-release properties	1734:1770	superior sustained-release properties	1734:1770	In addition, the OMMT/Alg composite hydrogel beads displayed superior sustained-release properties than Na-MMT/Alg, mainly ascribed to the good affinity of OMMT to hydrophobic drug that retarded the drug diffusion.
32208326	2	90	theme	nonylphenol	547:557	arg1	NPE					582:584	NPE	582:584	NPE	582:584	The OMMT was synthesized through the intercalation modification of sodium montmorillonite (Na-MMT) with cationic cetyltrimethylammonium bromide (CTAB), nonionic nonylphenol polyoxyethylene ether (NPE) and the mixture of them via simple and convenient wet ball-milling method.
32208326	2	90	theme	nonylphenol	547:557	arg1	ether					575:579	nonionic nonylphenol polyoxyethylene ether	538:579	nonionic nonylphenol polyoxyethylene ether (NPE)	538:585	The OMMT was synthesized through the intercalation modification of sodium montmorillonite (Na-MMT) with cationic cetyltrimethylammonium bromide (CTAB), nonionic nonylphenol polyoxyethylene ether (NPE) and the mixture of them via simple and convenient wet ball-milling method.
32208326	8	91	with	CTA/NPE-MMT/Alg	1903:1917	arg1	EE					1988:1989	EE	1988:1989	EE	1988:1989	In particular, CTA/NPE-MMT/Alg with the highest loading capacity (LC) and encapsulation efficiency (EE) revealed the optimal controlled performance for the release of hydrophobic ibuprofen.
32208326	8	91	with	CTA/NPE-MMT/Alg	1903:1917	arg1	LC					1954:1955	LC	1954:1955	LC	1954:1955	In particular, CTA/NPE-MMT/Alg with the highest loading capacity (LC) and encapsulation efficiency (EE) revealed the optimal controlled performance for the release of hydrophobic ibuprofen.
32208326	8	91	with	CTA/NPE-MMT/Alg	1903:1917	arg1	capacity					1944:1951	the highest loading capacity	1924:1951	the highest loading capacity (LC)	1924:1956	In particular, CTA/NPE-MMT/Alg with the highest loading capacity (LC) and encapsulation efficiency (EE) revealed the optimal controlled performance for the release of hydrophobic ibuprofen.
32208326	8	91	with	CTA/NPE-MMT/Alg	1903:1917	arg1	efficiency					1976:1985	encapsulation efficiency	1962:1985	encapsulation efficiency (EE)	1962:1990	In particular, CTA/NPE-MMT/Alg with the highest loading capacity (LC) and encapsulation efficiency (EE) revealed the optimal controlled performance for the release of hydrophobic ibuprofen.
32208326	8	92	theme	hydrophobic	2055:2065	arg1	ibuprofen					2067:2075	hydrophobic ibuprofen	2055:2075	hydrophobic ibuprofen	2055:2075	In particular, CTA/NPE-MMT/Alg with the highest loading capacity (LC) and encapsulation efficiency (EE) revealed the optimal controlled performance for the release of hydrophobic ibuprofen.
32208326	6	93	theme	hydrophobic	1645:1655	arg1	molecules					1662:1670	the hydrophobic drug molecules	1641:1670	the hydrophobic drug molecules	1641:1670	And the cationic CTAB and nonionic NPE were adsorbed or intercalated into the MMT lamellar structure through the wet ball-milling process, which could change the hydrophilic nature of Na-MMT and improve its affinity to the hydrophobic drug molecules.
32208326	4	94	theme	adsorption	1241:1250	arg1	experiments					1252:1262	drug adsorption experiments	1236:1262	drug adsorption experiments	1236:1262	The physical and chemical properties of OMMT were comparatively evaluated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analyzer (TGA), BET-specific surface area measurements, and drug adsorption experiments.
32208326	7	95	theme	composite	1699:1707	arg1	beads					1718:1722	the OMMT/Alg composite hydrogel beads	1686:1722	the OMMT/Alg composite hydrogel beads	1686:1722	In addition, the OMMT/Alg composite hydrogel beads displayed superior sustained-release properties than Na-MMT/Alg, mainly ascribed to the good affinity of OMMT to hydrophobic drug that retarded the drug diffusion.
32208326	10	96	from	material	2373:2380	arg1	delivery					2410:2417	hydrophobic drug delivery	2393:2417	hydrophobic drug delivery	2393:2417	Based on the high drug loading capacity and excellent controlled drug release properties, the CTA/NPE-MMT/Alg incorporating hydrophobic drugs into hydrophilic matrices could be a highly promising material for use in hydrophobic drug delivery.
32208326	3	97	theme	organo-montmorillonite/alginate	679:709	arg1	beads					741:745	the organo-montmorillonite/alginate (OMMT/Alg) composite hydrogel beads	675:745	the organo-montmorillonite/alginate (OMMT/Alg) composite hydrogel beads with slow and controlled release properties	675:789	Furthermore, the organo-montmorillonite/alginate (OMMT/Alg) composite hydrogel beads with slow and controlled release properties were constructed by using alginate as a coating material under the exogenous cross-linking of calcium ions.
32208326	2	98	theme	sodium	453:458	arg1	Na-MMT					477:482	Na-MMT	477:482	Na-MMT	477:482	The OMMT was synthesized through the intercalation modification of sodium montmorillonite (Na-MMT) with cationic cetyltrimethylammonium bromide (CTAB), nonionic nonylphenol polyoxyethylene ether (NPE) and the mixture of them via simple and convenient wet ball-milling method.
32208326	2	98	theme	sodium	453:458	arg1	montmorillonite					460:474	sodium montmorillonite	453:474	sodium montmorillonite (Na-MMT)	453:483	The OMMT was synthesized through the intercalation modification of sodium montmorillonite (Na-MMT) with cationic cetyltrimethylammonium bromide (CTAB), nonionic nonylphenol polyoxyethylene ether (NPE) and the mixture of them via simple and convenient wet ball-milling method.
32208326	6	99	theme	nonionic	1448:1455	arg1	NPE					1457:1459	nonionic NPE	1448:1459	nonionic NPE	1448:1459	And the cationic CTAB and nonionic NPE were adsorbed or intercalated into the MMT lamellar structure through the wet ball-milling process, which could change the hydrophilic nature of Na-MMT and improve its affinity to the hydrophobic drug molecules.
32208326	1	100	theme	combined	326:333	arg1	use					335:337	the combined use	322:337	the combined use of organo-montmorillonite (OMMT) and alginate	322:383	The research goal of the present study was to develop a carrier for loading and controlled -release of the hydrophobic drug with the combined use of organo-montmorillonite (OMMT) and alginate.
32208326	4	101	theme	X-ray	1061:1065	arg1	XRD					1080:1082	XRD	1080:1082	XRD	1080:1082	The physical and chemical properties of OMMT were comparatively evaluated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analyzer (TGA), BET-specific surface area measurements, and drug adsorption experiments.
32208326	4	101	theme	X-ray	1061:1065	arg1	diffraction					1067:1077	X-ray diffraction	1061:1077	X-ray diffraction (XRD)	1061:1083	The physical and chemical properties of OMMT were comparatively evaluated by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analyzer (TGA), BET-specific surface area measurements, and drug adsorption experiments.
32208326	3	102	theme	composite	722:730	arg1	beads					741:745	the organo-montmorillonite/alginate (OMMT/Alg) composite hydrogel beads	675:745	the organo-montmorillonite/alginate (OMMT/Alg) composite hydrogel beads with slow and controlled release properties	675:789	Furthermore, the organo-montmorillonite/alginate (OMMT/Alg) composite hydrogel beads with slow and controlled release properties were constructed by using alginate as a coating material under the exogenous cross-linking of calcium ions.
32208326	3	103	theme	OMMT/Alg	712:719	arg1	beads					741:745	the organo-montmorillonite/alginate (OMMT/Alg) composite hydrogel beads	675:745	the organo-montmorillonite/alginate (OMMT/Alg) composite hydrogel beads with slow and controlled release properties	675:789	Furthermore, the organo-montmorillonite/alginate (OMMT/Alg) composite hydrogel beads with slow and controlled release properties were constructed by using alginate as a coating material under the exogenous cross-linking of calcium ions.
32208326	10	104	theme	release	2247:2253	arg1	properties					2255:2264	excellent controlled drug release properties	2221:2264	excellent controlled drug release properties	2221:2264	Based on the high drug loading capacity and excellent controlled drug release properties, the CTA/NPE-MMT/Alg incorporating hydrophobic drugs into hydrophilic matrices could be a highly promising material for use in hydrophobic drug delivery.
32208326	7	105	theme	drug	1872:1875	arg1	diffusion					1877:1885	the drug diffusion	1868:1885	the drug diffusion	1868:1885	In addition, the OMMT/Alg composite hydrogel beads displayed superior sustained-release properties than Na-MMT/Alg, mainly ascribed to the good affinity of OMMT to hydrophobic drug that retarded the drug diffusion.
32208326	6	106	theme	cationic	1430:1437	arg1	CTAB					1439:1442	the cationic CTAB	1426:1442	the cationic CTAB	1426:1442	And the cationic CTAB and nonionic NPE were adsorbed or intercalated into the MMT lamellar structure through the wet ball-milling process, which could change the hydrophilic nature of Na-MMT and improve its affinity to the hydrophobic drug molecules.
32208326	3	107	theme	slow	752:755	arg1	properties					780:789	slow and controlled release properties	752:789	slow and controlled release properties	752:789	Furthermore, the organo-montmorillonite/alginate (OMMT/Alg) composite hydrogel beads with slow and controlled release properties were constructed by using alginate as a coating material under the exogenous cross-linking of calcium ions.
32208326	5	108	theme	cation	1396:1401	arg1	reaction					1412:1419	the cation exchange reaction	1392:1419	the cation exchange reaction	1392:1419	Experimental results showed that the presence of CTAB was able to facilitate the intercalation of CTAB/NPE into Na-MMT through the cation exchange reaction.
32208326	6	109	theme	MMT	1500:1502	arg1	structure					1513:1521	the MMT lamellar structure	1496:1521	the MMT lamellar structure	1496:1521	And the cationic CTAB and nonionic NPE were adsorbed or intercalated into the MMT lamellar structure through the wet ball-milling process, which could change the hydrophilic nature of Na-MMT and improve its affinity to the hydrophobic drug molecules.
32208326	1	110	theme	controlled	273:282	arg1	-release					284:291	controlled -release	273:291	controlled -release	273:291	The research goal of the present study was to develop a carrier for loading and controlled -release of the hydrophobic drug with the combined use of organo-montmorillonite (OMMT) and alginate.
32752178	0	0	theme	Gut	103:105	arg1	Modulation					118:127	Gut Microbiota Modulation	103:127	Gut Microbiota Modulation	103:127	Lactobacillus plantarum PS128 Improves Physiological Adaptation and Performance in Triathletes through Gut Microbiota Modulation.
32752178	10	1	from	difference	1528:1537	arg1	VO2max					1570:1575	VO2max	1570:1575	VO2max	1570:1575	PS128 significantly increased the athletes' endurance, by about 130% as compared to the PG group, but there was no significant difference in maximal oxygen consumption (VO2max) and composition between groups.
32752178	10	1	from	difference	1528:1537	arg1	composition					1582:1592	composition	1582:1592	composition	1582:1592	PS128 significantly increased the athletes' endurance, by about 130% as compared to the PG group, but there was no significant difference in maximal oxygen consumption (VO2max) and composition between groups.
32752178	10	1	from	difference	1528:1537	arg1	consumption					1557:1567	maximal oxygen consumption	1542:1567	maximal oxygen consumption (VO2max)	1542:1576	PS128 significantly increased the athletes' endurance, by about 130% as compared to the PG group, but there was no significant difference in maximal oxygen consumption (VO2max) and composition between groups.
32752178	7	2	theme	colony-forming	1122:1135	arg1	CFU					1144:1146	CFU	1144:1146	CFU	1144:1146	The triathletes were assigned to two groups: an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day) and a placebo group (PG).
32752178	7	2	theme	colony-forming	1122:1135	arg1	units					1137:1141	colony-forming units	1122:1141	3 × 1010 colony-forming units (CFU)/day	1113:1151	The triathletes were assigned to two groups: an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day) and a placebo group (PG).
32752178	13	3	theme	maximal	2329:2335	arg1	uptake					2344:2349	maximal oxygen uptake	2329:2349	maximal oxygen uptake	2329:2349	In conclusion, PS128 supplementation was associated with an improvement on endurance running performance through microbiota modulation and related metabolites, but not in maximal oxygen uptake.
32752178	3	4	theme	cancer	521:526	arg1	prevention					528:537	cancer prevention	521:537	cancer prevention	521:537	Probiotics, prebiotics, and synbiotics have been reported to have health-promoting activities (e.g., immunoregulation and cancer prevention).
32752178	6	5	theme	PS128	921:925	arg1	supplementation					927:941	L. plantarum PS128 supplementation	908:941	L. plantarum PS128 supplementation	908:941	Thus, here we investigate how the microbiota of triathletes are altered by L. plantarum PS128 supplementation, not only for exercise performance but also for possible physiological adaptation.
32752178	7	6	dep	group	1102:1106	arg1	plantarum					1077:1085	an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day)	1071:1152	an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day)	1071:1152	The triathletes were assigned to two groups: an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day) and a placebo group (PG).
32752178	7	7	theme	units	1137:1141	arg1	/day					1148:1151	3 × 1010 colony-forming units (CFU)/day	1113:1151	3 × 1010 colony-forming units (CFU)/day	1113:1151	The triathletes were assigned to two groups: an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day) and a placebo group (PG).
32752178	11	8	dep	decreases	1698:1706	arg1	Desulfovibrio					1758:1770	Desulfovibrio	1758:1770	Desulfovibrio	1758:1770	The PS128 supplementation (LG) modulated the athlete's microbiota with both significant decreases (Anaerotruncus, Caproiciproducens, Coprobacillus, Desulfovibrio, Dielma, Family_XIII, Holdemania, and Oxalobacter) and increases (Akkermansia, Bifidobacterium, Butyricimonas, and Lactobacillus), and the LG showed lower diversity when compared to the PG.
32752178	11	8	dep	decreases	1698:1706	arg1	Holdemania					1794:1803	Holdemania	1794:1803	Holdemania	1794:1803	The PS128 supplementation (LG) modulated the athlete's microbiota with both significant decreases (Anaerotruncus, Caproiciproducens, Coprobacillus, Desulfovibrio, Dielma, Family_XIII, Holdemania, and Oxalobacter) and increases (Akkermansia, Bifidobacterium, Butyricimonas, and Lactobacillus), and the LG showed lower diversity when compared to the PG.
32752178	11	8	dep	decreases	1698:1706	arg1	Caproiciproducens					1724:1740	Caproiciproducens	1724:1740	Caproiciproducens	1724:1740	The PS128 supplementation (LG) modulated the athlete's microbiota with both significant decreases (Anaerotruncus, Caproiciproducens, Coprobacillus, Desulfovibrio, Dielma, Family_XIII, Holdemania, and Oxalobacter) and increases (Akkermansia, Bifidobacterium, Butyricimonas, and Lactobacillus), and the LG showed lower diversity when compared to the PG.
32752178	11	8	dep	decreases	1698:1706	arg1	Dielma					1773:1778	Dielma	1773:1778	Dielma	1773:1778	The PS128 supplementation (LG) modulated the athlete's microbiota with both significant decreases (Anaerotruncus, Caproiciproducens, Coprobacillus, Desulfovibrio, Dielma, Family_XIII, Holdemania, and Oxalobacter) and increases (Akkermansia, Bifidobacterium, Butyricimonas, and Lactobacillus), and the LG showed lower diversity when compared to the PG.
32752178	11	8	dep	decreases	1698:1706	arg1	Oxalobacter					1810:1820	Oxalobacter	1810:1820	Oxalobacter	1810:1820	The PS128 supplementation (LG) modulated the athlete's microbiota with both significant decreases (Anaerotruncus, Caproiciproducens, Coprobacillus, Desulfovibrio, Dielma, Family_XIII, Holdemania, and Oxalobacter) and increases (Akkermansia, Bifidobacterium, Butyricimonas, and Lactobacillus), and the LG showed lower diversity when compared to the PG.
32752178	11	8	dep	decreases	1698:1706	arg1	decreases					1698:1706	significant decreases	1686:1706	significant decreases (Anaerotruncus, Caproiciproducens, Coprobacillus, Desulfovibrio, Dielma, Family_XIII, Holdemania, and Oxalobacter)	1686:1821	The PS128 supplementation (LG) modulated the athlete's microbiota with both significant decreases (Anaerotruncus, Caproiciproducens, Coprobacillus, Desulfovibrio, Dielma, Family_XIII, Holdemania, and Oxalobacter) and increases (Akkermansia, Bifidobacterium, Butyricimonas, and Lactobacillus), and the LG showed lower diversity when compared to the PG.
32752178	11	8	dep	decreases	1698:1706	arg1	Coprobacillus					1743:1755	Coprobacillus	1743:1755	Coprobacillus	1743:1755	The PS128 supplementation (LG) modulated the athlete's microbiota with both significant decreases (Anaerotruncus, Caproiciproducens, Coprobacillus, Desulfovibrio, Dielma, Family_XIII, Holdemania, and Oxalobacter) and increases (Akkermansia, Bifidobacterium, Butyricimonas, and Lactobacillus), and the LG showed lower diversity when compared to the PG.
32752178	7	9	dep	groups	1063:1068	arg1	PG					1175:1176	PG	1175:1176	PG	1175:1176	The triathletes were assigned to two groups: an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day) and a placebo group (PG).
32752178	7	9	dep	groups	1063:1068	arg1	group					1168:1172	a placebo group	1158:1172	a placebo group (PG)	1158:1177	The triathletes were assigned to two groups: an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day) and a placebo group (PG).
32752178	7	9	dep	groups	1063:1068	arg1	LG					1109:1110	LG	1109:1110	LG	1109:1110	The triathletes were assigned to two groups: an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day) and a placebo group (PG).
32752178	7	9	dep	groups	1063:1068	arg1	groups					1063:1068	two groups	1059:1068	two groups: an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day) and a placebo group (PG)	1059:1177	The triathletes were assigned to two groups: an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day) and a placebo group (PG).
32752178	7	9	dep	groups	1063:1068	arg1	group					1102:1106	128 supplement group	1087:1106	an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day)	1071:1152	The triathletes were assigned to two groups: an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day) and a placebo group (PG).
32752178	13	10	theme	endurance	2233:2241	arg1	performance					2251:2261	endurance running performance	2233:2261	endurance running performance	2233:2261	In conclusion, PS128 supplementation was associated with an improvement on endurance running performance through microbiota modulation and related metabolites, but not in maximal oxygen uptake.
32752178	7	11	theme	×	1115:1115	arg1	/day					1148:1151	3 × 1010 colony-forming units (CFU)/day	1113:1151	3 × 1010 colony-forming units (CFU)/day	1113:1151	The triathletes were assigned to two groups: an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day) and a placebo group (PG).
32752178	3	12	dep	immunoregulation	500:515	arg1	e.g.					494:497	e.g.	494:497	e.g.	494:497	Probiotics, prebiotics, and synbiotics have been reported to have health-promoting activities (e.g., immunoregulation and cancer prevention).
32752178	0	13	theme	Microbiota	107:116	arg1	Modulation					118:127	Gut Microbiota Modulation	103:127	Gut Microbiota Modulation	103:127	Lactobacillus plantarum PS128 Improves Physiological Adaptation and Performance in Triathletes through Gut Microbiota Modulation.
32752178	0	14	from	Performance	68:78	arg1	Triathletes					83:93	Triathletes	83:93	Triathletes	83:93	Lactobacillus plantarum PS128 Improves Physiological Adaptation and Performance in Triathletes through Gut Microbiota Modulation.
32752178	5	15	theme	previous	685:692	arg1	study					694:698	our previous study	681:698	our previous study	681:698	In our previous study, we found that Lactobacillus plantarum PS128 could ameliorate inflammation and oxidative stress, with improved exercise performance.
32752178	12	16	theme	microbiota	2146:2155	arg1	modulation					2117:2126	a modulation	2115:2126	a modulation of the associated microbiota	2115:2155	Also, the short-chain fatty acids (SCFAs; acetate, propionate, and butyrate) of the LG were significantly higher than the PG, which might be a result of a modulation of the associated microbiota.
32752178	2	17	theme	optimum	367:373	arg1	health					375:380	optimum health	367:380	optimum health	367:380	A triathlete's microbiome might be modulated by diet, age, medical treatments, lifestyle, and exercise, thereby maintaining aerobiosis and optimum health and performance.
32752178	12	18	theme	associated	2135:2144	arg1	microbiota					2146:2155	the associated microbiota	2131:2155	the associated microbiota	2131:2155	Also, the short-chain fatty acids (SCFAs; acetate, propionate, and butyrate) of the LG were significantly higher than the PG, which might be a result of a modulation of the associated microbiota.
32752178	6	19	theme	plantarum	911:919	arg1	supplementation					927:941	L. plantarum PS128 supplementation	908:941	L. plantarum PS128 supplementation	908:941	Thus, here we investigate how the microbiota of triathletes are altered by L. plantarum PS128 supplementation, not only for exercise performance but also for possible physiological adaptation.
32752178	2	20	theme	medical	287:293	arg1	treatments					295:304	medical treatments	287:304	medical treatments	287:304	A triathlete's microbiome might be modulated by diet, age, medical treatments, lifestyle, and exercise, thereby maintaining aerobiosis and optimum health and performance.
32752178	8	21	theme	exercise	1221:1228	arg1	training					1230:1237	their regular exercise training	1207:1237	their regular exercise training for the next 4 weeks	1207:1258	Both groups continued with their regular exercise training for the next 4 weeks.
32752178	0	22	from	Adaptation	53:62	arg1	Triathletes					83:93	Triathletes	83:93	Triathletes	83:93	Lactobacillus plantarum PS128 Improves Physiological Adaptation and Performance in Triathletes through Gut Microbiota Modulation.
32752178	11	23	theme	significant	1686:1696	arg1	Desulfovibrio					1758:1770	Desulfovibrio	1758:1770	Desulfovibrio	1758:1770	The PS128 supplementation (LG) modulated the athlete's microbiota with both significant decreases (Anaerotruncus, Caproiciproducens, Coprobacillus, Desulfovibrio, Dielma, Family_XIII, Holdemania, and Oxalobacter) and increases (Akkermansia, Bifidobacterium, Butyricimonas, and Lactobacillus), and the LG showed lower diversity when compared to the PG.
32752178	11	23	theme	significant	1686:1696	arg1	Holdemania					1794:1803	Holdemania	1794:1803	Holdemania	1794:1803	The PS128 supplementation (LG) modulated the athlete's microbiota with both significant decreases (Anaerotruncus, Caproiciproducens, Coprobacillus, Desulfovibrio, Dielma, Family_XIII, Holdemania, and Oxalobacter) and increases (Akkermansia, Bifidobacterium, Butyricimonas, and Lactobacillus), and the LG showed lower diversity when compared to the PG.
32752178	11	23	theme	significant	1686:1696	arg1	Caproiciproducens					1724:1740	Caproiciproducens	1724:1740	Caproiciproducens	1724:1740	The PS128 supplementation (LG) modulated the athlete's microbiota with both significant decreases (Anaerotruncus, Caproiciproducens, Coprobacillus, Desulfovibrio, Dielma, Family_XIII, Holdemania, and Oxalobacter) and increases (Akkermansia, Bifidobacterium, Butyricimonas, and Lactobacillus), and the LG showed lower diversity when compared to the PG.
32752178	11	23	theme	significant	1686:1696	arg1	Dielma					1773:1778	Dielma	1773:1778	Dielma	1773:1778	The PS128 supplementation (LG) modulated the athlete's microbiota with both significant decreases (Anaerotruncus, Caproiciproducens, Coprobacillus, Desulfovibrio, Dielma, Family_XIII, Holdemania, and Oxalobacter) and increases (Akkermansia, Bifidobacterium, Butyricimonas, and Lactobacillus), and the LG showed lower diversity when compared to the PG.
32752178	11	23	theme	significant	1686:1696	arg1	Oxalobacter					1810:1820	Oxalobacter	1810:1820	Oxalobacter	1810:1820	The PS128 supplementation (LG) modulated the athlete's microbiota with both significant decreases (Anaerotruncus, Caproiciproducens, Coprobacillus, Desulfovibrio, Dielma, Family_XIII, Holdemania, and Oxalobacter) and increases (Akkermansia, Bifidobacterium, Butyricimonas, and Lactobacillus), and the LG showed lower diversity when compared to the PG.
32752178	11	23	theme	significant	1686:1696	arg1	decreases					1698:1706	significant decreases	1686:1706	significant decreases (Anaerotruncus, Caproiciproducens, Coprobacillus, Desulfovibrio, Dielma, Family_XIII, Holdemania, and Oxalobacter)	1686:1821	The PS128 supplementation (LG) modulated the athlete's microbiota with both significant decreases (Anaerotruncus, Caproiciproducens, Coprobacillus, Desulfovibrio, Dielma, Family_XIII, Holdemania, and Oxalobacter) and increases (Akkermansia, Bifidobacterium, Butyricimonas, and Lactobacillus), and the LG showed lower diversity when compared to the PG.
32752178	11	23	theme	significant	1686:1696	arg1	Coprobacillus					1743:1755	Coprobacillus	1743:1755	Coprobacillus	1743:1755	The PS128 supplementation (LG) modulated the athlete's microbiota with both significant decreases (Anaerotruncus, Caproiciproducens, Coprobacillus, Desulfovibrio, Dielma, Family_XIII, Holdemania, and Oxalobacter) and increases (Akkermansia, Bifidobacterium, Butyricimonas, and Lactobacillus), and the LG showed lower diversity when compared to the PG.
32752178	7	24	theme	placebo	1160:1166	arg1	PG					1175:1176	PG	1175:1176	PG	1175:1176	The triathletes were assigned to two groups: an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day) and a placebo group (PG).
32752178	7	24	theme	placebo	1160:1166	arg1	group					1168:1172	a placebo group	1158:1172	a placebo group (PG)	1158:1177	The triathletes were assigned to two groups: an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day) and a placebo group (PG).
32752178	7	24	theme	placebo	1160:1166	arg1	groups					1063:1068	two groups	1059:1068	two groups: an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day) and a placebo group (PG)	1059:1177	The triathletes were assigned to two groups: an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day) and a placebo group (PG).
32752178	0	25	theme	plantarum	14:22	arg1	PS128					24:28	Lactobacillus plantarum PS128	0:28	Lactobacillus plantarum PS128	0:28	Lactobacillus plantarum PS128 Improves Physiological Adaptation and Performance in Triathletes through Gut Microbiota Modulation.
32752178	9	26	theme	body	1288:1291	arg1	composition					1293:1303	body composition	1288:1303	body composition	1288:1303	The endurance performance, body composition, biochemistries, blood cells, microbiota, and associated metabolites were further investigated.
32752178	10	27	theme	maximal	1542:1548	arg1	VO2max					1570:1575	VO2max	1570:1575	VO2max	1570:1575	PS128 significantly increased the athletes' endurance, by about 130% as compared to the PG group, but there was no significant difference in maximal oxygen consumption (VO2max) and composition between groups.
32752178	10	27	theme	maximal	1542:1548	arg1	consumption					1557:1567	maximal oxygen consumption	1542:1567	maximal oxygen consumption (VO2max)	1542:1576	PS128 significantly increased the athletes' endurance, by about 130% as compared to the PG group, but there was no significant difference in maximal oxygen consumption (VO2max) and composition between groups.
32752178	9	28	theme	endurance	1265:1273	arg1	performance					1275:1285	The endurance performance	1261:1285	The endurance performance	1261:1285	The endurance performance, body composition, biochemistries, blood cells, microbiota, and associated metabolites were further investigated.
32752178	12	29	theme	modulation	2117:2126	arg1	result					2105:2110	a result	2103:2110	a result of a modulation of the associated microbiota	2103:2155	Also, the short-chain fatty acids (SCFAs; acetate, propionate, and butyrate) of the LG were significantly higher than the PG, which might be a result of a modulation of the associated microbiota.
32752178	12	29	theme	modulation	2117:2126	arg1	PG					2084:2085	the PG	2080:2085	the PG	2080:2085	Also, the short-chain fatty acids (SCFAs; acetate, propionate, and butyrate) of the LG were significantly higher than the PG, which might be a result of a modulation of the associated microbiota.
32752178	1	30	theme	physiological	202:214	arg1	adaptation					216:225	physiological adaptation	202:225	physiological adaptation	202:225	A triathlon is an extremely high-intensity exercise and a challenge for physiological adaptation.
32752178	0	31	theme	Lactobacillus	0:12	arg1	PS128					24:28	Lactobacillus plantarum PS128	0:28	Lactobacillus plantarum PS128	0:28	Lactobacillus plantarum PS128 Improves Physiological Adaptation and Performance in Triathletes through Gut Microbiota Modulation.
32752178	6	32	theme	physiological	1000:1012	arg1	adaptation					1014:1023	possible physiological adaptation	991:1023	possible physiological adaptation	991:1023	Thus, here we investigate how the microbiota of triathletes are altered by L. plantarum PS128 supplementation, not only for exercise performance but also for possible physiological adaptation.
32752178	10	33	theme	PG	1489:1490	arg1	group					1492:1496	the PG group	1485:1496	the PG group	1485:1496	PS128 significantly increased the athletes' endurance, by about 130% as compared to the PG group, but there was no significant difference in maximal oxygen consumption (VO2max) and composition between groups.
32752178	12	34	dep	SCFAs	1997:2001	arg1	propionate					2013:2022	propionate	2013:2022	propionate	2013:2022	Also, the short-chain fatty acids (SCFAs; acetate, propionate, and butyrate) of the LG were significantly higher than the PG, which might be a result of a modulation of the associated microbiota.
32752178	12	34	dep	SCFAs	1997:2001	arg1	butyrate					2029:2036	butyrate	2029:2036	butyrate	2029:2036	Also, the short-chain fatty acids (SCFAs; acetate, propionate, and butyrate) of the LG were significantly higher than the PG, which might be a result of a modulation of the associated microbiota.
32752178	12	34	dep	SCFAs	1997:2001	arg1	acetate					2004:2010	acetate	2004:2010	acetate	2004:2010	Also, the short-chain fatty acids (SCFAs; acetate, propionate, and butyrate) of the LG were significantly higher than the PG, which might be a result of a modulation of the associated microbiota.
32752178	12	34	dep	SCFAs	1997:2001	arg1	SCFAs					1997:2001	SCFAs	1997:2001	SCFAs; acetate, propionate, and butyrate	1997:2036	Also, the short-chain fatty acids (SCFAs; acetate, propionate, and butyrate) of the LG were significantly higher than the PG, which might be a result of a modulation of the associated microbiota.
32752178	12	35	dep	acids	1990:1994	arg1	propionate					2013:2022	propionate	2013:2022	propionate	2013:2022	Also, the short-chain fatty acids (SCFAs; acetate, propionate, and butyrate) of the LG were significantly higher than the PG, which might be a result of a modulation of the associated microbiota.
32752178	12	35	dep	acids	1990:1994	arg1	butyrate					2029:2036	butyrate	2029:2036	butyrate	2029:2036	Also, the short-chain fatty acids (SCFAs; acetate, propionate, and butyrate) of the LG were significantly higher than the PG, which might be a result of a modulation of the associated microbiota.
32752178	12	35	dep	acids	1990:1994	arg1	acetate					2004:2010	acetate	2004:2010	acetate	2004:2010	Also, the short-chain fatty acids (SCFAs; acetate, propionate, and butyrate) of the LG were significantly higher than the PG, which might be a result of a modulation of the associated microbiota.
32752178	12	35	dep	acids	1990:1994	arg1	SCFAs					1997:2001	SCFAs	1997:2001	SCFAs; acetate, propionate, and butyrate	1997:2036	Also, the short-chain fatty acids (SCFAs; acetate, propionate, and butyrate) of the LG were significantly higher than the PG, which might be a result of a modulation of the associated microbiota.
32752178	8	36	theme	regular	1213:1219	arg1	training					1230:1237	their regular exercise training	1207:1237	their regular exercise training for the next 4 weeks	1207:1258	Both groups continued with their regular exercise training for the next 4 weeks.
32752178	6	37	theme	possible	991:998	arg1	adaptation					1014:1023	possible physiological adaptation	991:1023	possible physiological adaptation	991:1023	Thus, here we investigate how the microbiota of triathletes are altered by L. plantarum PS128 supplementation, not only for exercise performance but also for possible physiological adaptation.
32752178	7	38	theme	supplement	1091:1100	arg1	groups					1063:1068	two groups	1059:1068	two groups: an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day) and a placebo group (PG)	1059:1177	The triathletes were assigned to two groups: an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day) and a placebo group (PG).
32752178	7	38	theme	supplement	1091:1100	arg1	LG					1109:1110	LG	1109:1110	LG	1109:1110	The triathletes were assigned to two groups: an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day) and a placebo group (PG).
32752178	7	38	theme	supplement	1091:1100	arg1	group					1102:1106	128 supplement group	1087:1106	an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day)	1071:1152	The triathletes were assigned to two groups: an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day) and a placebo group (PG).
32752178	5	39	theme	Lactobacillus	715:727	arg1	PS128					739:743	Lactobacillus plantarum PS128	715:743	Lactobacillus plantarum PS128	715:743	In our previous study, we found that Lactobacillus plantarum PS128 could ameliorate inflammation and oxidative stress, with improved exercise performance.
32752178	11	40	dep	increases	1827:1835	arg1	Butyricimonas					1868:1880	Butyricimonas	1868:1880	Butyricimonas	1868:1880	The PS128 supplementation (LG) modulated the athlete's microbiota with both significant decreases (Anaerotruncus, Caproiciproducens, Coprobacillus, Desulfovibrio, Dielma, Family_XIII, Holdemania, and Oxalobacter) and increases (Akkermansia, Bifidobacterium, Butyricimonas, and Lactobacillus), and the LG showed lower diversity when compared to the PG.
32752178	11	40	dep	increases	1827:1835	arg1	Lactobacillus					1887:1899	Lactobacillus	1887:1899	Lactobacillus	1887:1899	The PS128 supplementation (LG) modulated the athlete's microbiota with both significant decreases (Anaerotruncus, Caproiciproducens, Coprobacillus, Desulfovibrio, Dielma, Family_XIII, Holdemania, and Oxalobacter) and increases (Akkermansia, Bifidobacterium, Butyricimonas, and Lactobacillus), and the LG showed lower diversity when compared to the PG.
32752178	11	40	dep	increases	1827:1835	arg1	Bifidobacterium					1851:1865	Bifidobacterium	1851:1865	Bifidobacterium	1851:1865	The PS128 supplementation (LG) modulated the athlete's microbiota with both significant decreases (Anaerotruncus, Caproiciproducens, Coprobacillus, Desulfovibrio, Dielma, Family_XIII, Holdemania, and Oxalobacter) and increases (Akkermansia, Bifidobacterium, Butyricimonas, and Lactobacillus), and the LG showed lower diversity when compared to the PG.
32752178	11	40	dep	increases	1827:1835	arg1	Akkermansia					1838:1848	Akkermansia	1838:1848	Akkermansia	1838:1848	The PS128 supplementation (LG) modulated the athlete's microbiota with both significant decreases (Anaerotruncus, Caproiciproducens, Coprobacillus, Desulfovibrio, Dielma, Family_XIII, Holdemania, and Oxalobacter) and increases (Akkermansia, Bifidobacterium, Butyricimonas, and Lactobacillus), and the LG showed lower diversity when compared to the PG.
32752178	11	40	dep	increases	1827:1835	arg1	increases					1827:1835	increases	1827:1835	increases (Akkermansia, Bifidobacterium, Butyricimonas, and Lactobacillus)	1827:1900	The PS128 supplementation (LG) modulated the athlete's microbiota with both significant decreases (Anaerotruncus, Caproiciproducens, Coprobacillus, Desulfovibrio, Dielma, Family_XIII, Holdemania, and Oxalobacter) and increases (Akkermansia, Bifidobacterium, Butyricimonas, and Lactobacillus), and the LG showed lower diversity when compared to the PG.
32752178	13	41	theme	PS128	2173:2177	arg1	supplementation					2179:2193	PS128 supplementation	2173:2193	PS128 supplementation	2173:2193	In conclusion, PS128 supplementation was associated with an improvement on endurance running performance through microbiota modulation and related metabolites, but not in maximal oxygen uptake.
32752178	5	42	theme	improved	802:809	arg1	performance					820:830	improved exercise performance	802:830	improved exercise performance	802:830	In our previous study, we found that Lactobacillus plantarum PS128 could ameliorate inflammation and oxidative stress, with improved exercise performance.
32752178	6	43	theme	L.	908:909	arg1	supplementation					927:941	L. plantarum PS128 supplementation	908:941	L. plantarum PS128 supplementation	908:941	Thus, here we investigate how the microbiota of triathletes are altered by L. plantarum PS128 supplementation, not only for exercise performance but also for possible physiological adaptation.
32752178	5	44	theme	plantarum	729:737	arg1	PS128					739:743	Lactobacillus plantarum PS128	715:743	Lactobacillus plantarum PS128	715:743	In our previous study, we found that Lactobacillus plantarum PS128 could ameliorate inflammation and oxidative stress, with improved exercise performance.
32752178	3	45	contain	have	460:463	arg1	Probiotics					399:408	Probiotics	399:408	Probiotics	399:408	Probiotics, prebiotics, and synbiotics have been reported to have health-promoting activities (e.g., immunoregulation and cancer prevention).
32752178	3	45	contain	have	460:463	arg2	activities					482:491	health-promoting activities	465:491	health-promoting activities (e.g., immunoregulation and cancer prevention)	465:538	Probiotics, prebiotics, and synbiotics have been reported to have health-promoting activities (e.g., immunoregulation and cancer prevention).
32752178	3	45	contain	have	460:463	arg1	prebiotics					411:420	prebiotics	411:420	prebiotics	411:420	Probiotics, prebiotics, and synbiotics have been reported to have health-promoting activities (e.g., immunoregulation and cancer prevention).
32752178	3	45	contain	have	460:463	arg1	synbiotics					427:436	synbiotics	427:436	synbiotics	427:436	Probiotics, prebiotics, and synbiotics have been reported to have health-promoting activities (e.g., immunoregulation and cancer prevention).
32752178	8	46	theme	next	1247:1250	arg1	weeks					1254:1258	the next 4 weeks	1243:1258	the next 4 weeks	1243:1258	Both groups continued with their regular exercise training for the next 4 weeks.
32752178	0	47	theme	Physiological	39:51	arg1	Adaptation					53:62	Physiological Adaptation	39:62	Physiological Adaptation	39:62	Lactobacillus plantarum PS128 Improves Physiological Adaptation and Performance in Triathletes through Gut Microbiota Modulation.
32752178	9	48	theme	blood	1322:1326	arg1	cells					1328:1332	blood cells	1322:1332	blood cells	1322:1332	The endurance performance, body composition, biochemistries, blood cells, microbiota, and associated metabolites were further investigated.
32752178	5	49	theme	exercise	811:818	arg1	performance					820:830	improved exercise performance	802:830	improved exercise performance	802:830	In our previous study, we found that Lactobacillus plantarum PS128 could ameliorate inflammation and oxidative stress, with improved exercise performance.
32752178	6	50	theme	triathletes	881:891	arg1	microbiota					867:876	the microbiota	863:876	the microbiota of triathletes	863:891	Thus, here we investigate how the microbiota of triathletes are altered by L. plantarum PS128 supplementation, not only for exercise performance but also for possible physiological adaptation.
32752178	13	51	theme	microbiota	2271:2280	arg1	modulation					2282:2291	microbiota modulation	2271:2291	microbiota modulation	2271:2291	In conclusion, PS128 supplementation was associated with an improvement on endurance running performance through microbiota modulation and related metabolites, but not in maximal oxygen uptake.
32752178	4	52	theme	functional	655:664	arg1	activities					666:675	functional activities	655:675	functional activities	655:675	However, few studies have addressed how probiotics affect the microbiota of athletes and how this translates into functional activities.
32752178	10	53	theme	significant	1516:1526	arg1	difference					1528:1537	no significant difference	1513:1537	no significant difference in maximal oxygen consumption (VO2max) and composition between groups	1513:1607	PS128 significantly increased the athletes' endurance, by about 130% as compared to the PG group, but there was no significant difference in maximal oxygen consumption (VO2max) and composition between groups.
32752178	7	54	dep	LG	1109:1110	arg1	/day					1148:1151	3 × 1010 colony-forming units (CFU)/day	1113:1151	3 × 1010 colony-forming units (CFU)/day	1113:1151	The triathletes were assigned to two groups: an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day) and a placebo group (PG).
32752178	4	55	dep	addressed	567:575	arg1	translates					639:648	translates	639:648	translates into functional activities	639:675	However, few studies have addressed how probiotics affect the microbiota of athletes and how this translates into functional activities.
32752178	4	55	dep	addressed	567:575	arg1	affect					592:597	affect	592:597	affect the microbiota of athletes	592:624	However, few studies have addressed how probiotics affect the microbiota of athletes and how this translates into functional activities.
32752178	6	56	theme	exercise	957:964	arg1	performance					966:976	exercise performance	957:976	exercise performance	957:976	Thus, here we investigate how the microbiota of triathletes are altered by L. plantarum PS128 supplementation, not only for exercise performance but also for possible physiological adaptation.
32752178	7	57	theme	L.	1074:1075	arg1	groups					1063:1068	two groups	1059:1068	two groups: an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day) and a placebo group (PG)	1059:1177	The triathletes were assigned to two groups: an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day) and a placebo group (PG).
32752178	7	57	theme	L.	1074:1075	arg1	LG					1109:1110	LG	1109:1110	LG	1109:1110	The triathletes were assigned to two groups: an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day) and a placebo group (PG).
32752178	7	57	theme	L.	1074:1075	arg1	group					1102:1106	128 supplement group	1087:1106	an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day)	1071:1152	The triathletes were assigned to two groups: an L. plantarum 128 supplement group (LG, 3 × 1010 colony-forming units (CFU)/day) and a placebo group (PG).
32752178	12	58	theme	fatty	1984:1988	arg1	higher					2068:2073	higher	2068:2073	higher	2068:2073	Also, the short-chain fatty acids (SCFAs; acetate, propionate, and butyrate) of the LG were significantly higher than the PG, which might be a result of a modulation of the associated microbiota.
32752178	12	58	theme	fatty	1984:1988	arg1	acids					1990:1994	the short-chain fatty acids	1968:1994	the short-chain fatty acids (SCFAs; acetate, propionate, and butyrate) of the LG	1968:2047	Also, the short-chain fatty acids (SCFAs; acetate, propionate, and butyrate) of the LG were significantly higher than the PG, which might be a result of a modulation of the associated microbiota.
32752178	4	59	theme	few	550:552	arg1	studies					554:560	few studies	550:560	few studies	550:560	However, few studies have addressed how probiotics affect the microbiota of athletes and how this translates into functional activities.
32752178	12	60	theme	LG	2046:2047	arg1	higher					2068:2073	higher	2068:2073	higher	2068:2073	Also, the short-chain fatty acids (SCFAs; acetate, propionate, and butyrate) of the LG were significantly higher than the PG, which might be a result of a modulation of the associated microbiota.
32752178	12	60	theme	LG	2046:2047	arg1	acids					1990:1994	the short-chain fatty acids	1968:1994	the short-chain fatty acids (SCFAs; acetate, propionate, and butyrate) of the LG	1968:2047	Also, the short-chain fatty acids (SCFAs; acetate, propionate, and butyrate) of the LG were significantly higher than the PG, which might be a result of a modulation of the associated microbiota.
32752178	11	61	theme	PS128	1614:1618	arg1	LG					1637:1638	LG	1637:1638	LG	1637:1638	The PS128 supplementation (LG) modulated the athlete's microbiota with both significant decreases (Anaerotruncus, Caproiciproducens, Coprobacillus, Desulfovibrio, Dielma, Family_XIII, Holdemania, and Oxalobacter) and increases (Akkermansia, Bifidobacterium, Butyricimonas, and Lactobacillus), and the LG showed lower diversity when compared to the PG.
32752178	11	61	theme	PS128	1614:1618	arg1	supplementation					1620:1634	The PS128 supplementation	1610:1634	The PS128 supplementation (LG)	1610:1639	The PS128 supplementation (LG) modulated the athlete's microbiota with both significant decreases (Anaerotruncus, Caproiciproducens, Coprobacillus, Desulfovibrio, Dielma, Family_XIII, Holdemania, and Oxalobacter) and increases (Akkermansia, Bifidobacterium, Butyricimonas, and Lactobacillus), and the LG showed lower diversity when compared to the PG.
32752178	13	62	theme	oxygen	2337:2342	arg1	uptake					2344:2349	maximal oxygen uptake	2329:2349	maximal oxygen uptake	2329:2349	In conclusion, PS128 supplementation was associated with an improvement on endurance running performance through microbiota modulation and related metabolites, but not in maximal oxygen uptake.
32752178	11	63	theme	lower	1921:1925	arg1	diversity					1927:1935	lower diversity	1921:1935	lower diversity	1921:1935	The PS128 supplementation (LG) modulated the athlete's microbiota with both significant decreases (Anaerotruncus, Caproiciproducens, Coprobacillus, Desulfovibrio, Dielma, Family_XIII, Holdemania, and Oxalobacter) and increases (Akkermansia, Bifidobacterium, Butyricimonas, and Lactobacillus), and the LG showed lower diversity when compared to the PG.
32752178	4	64	theme	athletes	617:624	arg1	microbiota					603:612	the microbiota	599:612	the microbiota of athletes	599:624	However, few studies have addressed how probiotics affect the microbiota of athletes and how this translates into functional activities.
32752178	5	65	theme	oxidative	779:787	arg1	stress					789:794	oxidative stress	779:794	oxidative stress	779:794	In our previous study, we found that Lactobacillus plantarum PS128 could ameliorate inflammation and oxidative stress, with improved exercise performance.
32752178	3	66	theme	health-promoting	465:480	arg1	activities					482:491	health-promoting activities	465:491	health-promoting activities (e.g., immunoregulation and cancer prevention)	465:538	Probiotics, prebiotics, and synbiotics have been reported to have health-promoting activities (e.g., immunoregulation and cancer prevention).
32752178	13	67	theme	running	2243:2249	arg1	performance					2251:2261	endurance running performance	2233:2261	endurance running performance	2233:2261	In conclusion, PS128 supplementation was associated with an improvement on endurance running performance through microbiota modulation and related metabolites, but not in maximal oxygen uptake.
32752178	13	68	from	improvement	2218:2228	arg1	performance					2251:2261	endurance running performance	2233:2261	endurance running performance	2233:2261	In conclusion, PS128 supplementation was associated with an improvement on endurance running performance through microbiota modulation and related metabolites, but not in maximal oxygen uptake.
32752178	1	69	theme	high-intensity	158:171	arg1	exercise					173:180	an extremely high-intensity exercise	145:180	an extremely high-intensity exercise	145:180	A triathlon is an extremely high-intensity exercise and a challenge for physiological adaptation.
32752178	1	69	theme	high-intensity	158:171	arg1	triathlon					132:140	A triathlon	130:140	A triathlon	130:140	A triathlon is an extremely high-intensity exercise and a challenge for physiological adaptation.
32752178	9	70	theme	associated	1351:1360	arg1	metabolites					1362:1372	associated metabolites	1351:1372	associated metabolites	1351:1372	The endurance performance, body composition, biochemistries, blood cells, microbiota, and associated metabolites were further investigated.
32752178	13	71	theme	related	2297:2303	arg1	metabolites					2305:2315	related metabolites	2297:2315	related metabolites	2297:2315	In conclusion, PS128 supplementation was associated with an improvement on endurance running performance through microbiota modulation and related metabolites, but not in maximal oxygen uptake.
32752178	12	72	theme	short-chain	1972:1982	arg1	higher					2068:2073	higher	2068:2073	higher	2068:2073	Also, the short-chain fatty acids (SCFAs; acetate, propionate, and butyrate) of the LG were significantly higher than the PG, which might be a result of a modulation of the associated microbiota.
32752178	12	72	theme	short-chain	1972:1982	arg1	acids					1990:1994	the short-chain fatty acids	1968:1994	the short-chain fatty acids (SCFAs; acetate, propionate, and butyrate) of the LG	1968:2047	Also, the short-chain fatty acids (SCFAs; acetate, propionate, and butyrate) of the LG were significantly higher than the PG, which might be a result of a modulation of the associated microbiota.
32752178	10	73	theme	oxygen	1550:1555	arg1	VO2max					1570:1575	VO2max	1570:1575	VO2max	1570:1575	PS128 significantly increased the athletes' endurance, by about 130% as compared to the PG group, but there was no significant difference in maximal oxygen consumption (VO2max) and composition between groups.
32752178	10	73	theme	oxygen	1550:1555	arg1	consumption					1557:1567	maximal oxygen consumption	1542:1567	maximal oxygen consumption (VO2max)	1542:1576	PS128 significantly increased the athletes' endurance, by about 130% as compared to the PG group, but there was no significant difference in maximal oxygen consumption (VO2max) and composition between groups.
33404253	3	0	theme	glycoproteomic	632:645	arg1	approaches					647:656	electrophoretic, proteomic, and glycoproteomic approaches	600:656	electrophoretic, proteomic, and glycoproteomic approaches	600:656	Here, we characterized B. alcatraz venom using electrophoretic, proteomic, and glycoproteomic approaches.
33404253	7	1	theme	venom	1453:1457	arg1	N-terminome					1430:1440	the N-terminome	1426:1440	the N-terminome of a snake venom using the terminal amine isotopic labeling of substrates (TAILS) approach	1426:1531	Furthermore, we prospected for the first time the N-terminome of a snake venom using the terminal amine isotopic labeling of substrates (TAILS) approach and report the presence of ∼30% of N-termini corresponding to truncated toxin forms and ∼37% N-terminal sequences blocked by pyroglutamic acid in B. alcatraz venom.
33404253	4	2	theme	N-glycosylation	844:858	arg1	sites					860:864	N-glycosylation sites	844:864	N-glycosylation sites	844:864	Electrophoresis showed that B. alcatraz venom differs from B. jararaca and B. insularis; however, N-glycan removal revealed similarities between them, indicating that the occupation of N-glycosylation sites contributes to interspecies variability in the B. jararaca complex.
33404253	7	3	theme	first	1415:1419	arg1	time					1421:1424	the first time	1411:1424	the first time	1411:1424	Furthermore, we prospected for the first time the N-terminome of a snake venom using the terminal amine isotopic labeling of substrates (TAILS) approach and report the presence of ∼30% of N-termini corresponding to truncated toxin forms and ∼37% N-terminal sequences blocked by pyroglutamic acid in B. alcatraz venom.
33404253	1	4	theme	Bothrops	173:180	arg1	alcatraz					182:189	Bothrops alcatraz	173:189	Bothrops alcatraz	173:189	Bothrops alcatraz, a species endemic to Alcatrazes Islands, is regarded as critically endangered due to its small area of occurrence and the declining quality of its habitat.
33404253	1	4	theme	Bothrops	173:180	arg1	species					194:200	a species	192:200	a species endemic to Alcatrazes Islands	192:230	Bothrops alcatraz, a species endemic to Alcatrazes Islands, is regarded as critically endangered due to its small area of occurrence and the declining quality of its habitat.
33404253	5	5	dep	B.	996:997	arg1	alcatraz					999:1006	alcatraz	999:1006	alcatraz	999:1006	Metalloproteinase was the major toxin class identified in the B. alcatraz venom proteome followed by serine proteinase and C-type lectin, and overall, the adult B. alcatraz venom resembles that of B. jararaca juvenile specimens.
33404253	8	6	theme	shaping	1867:1873	arg1	phenotypes					1881:1890	shaping venom phenotypes	1867:1890	shaping venom phenotypes	1867:1890	These findings underscore a low correlation between venom gland transcriptomes and proteomes and support the view that post-translational processes play a major role in shaping venom phenotypes.
33404253	4	7	gly	N-glycosylation	844:858	arg2	sites					860:864	N-glycosylation sites	844:864	N-glycosylation sites	844:864	Electrophoresis showed that B. alcatraz venom differs from B. jararaca and B. insularis; however, N-glycan removal revealed similarities between them, indicating that the occupation of N-glycosylation sites contributes to interspecies variability in the B. jararaca complex.
33404253	0	8	theme	N-Terminome	107:117	arg1	Characterization					58:73	Characterization	58:73	Characterization of Proteome, Glycoproteome, and N-Terminome Using Terminal Amine Isotopic Labeling of Substrates	58:170	Venom Profiling of the Insular Species Bothrops alcatraz: Characterization of Proteome, Glycoproteome, and N-Terminome Using Terminal Amine Isotopic Labeling of Substrates.
33404253	7	9	theme	truncated	1595:1603	arg1	forms					1611:1615	truncated toxin forms	1595:1615	truncated toxin forms	1595:1615	Furthermore, we prospected for the first time the N-terminome of a snake venom using the terminal amine isotopic labeling of substrates (TAILS) approach and report the presence of ∼30% of N-termini corresponding to truncated toxin forms and ∼37% N-terminal sequences blocked by pyroglutamic acid in B. alcatraz venom.
33404253	4	10	theme	B.	913:914	arg1	complex					925:931	the B. jararaca complex	909:931	the B. jararaca complex	909:931	Electrophoresis showed that B. alcatraz venom differs from B. jararaca and B. insularis; however, N-glycan removal revealed similarities between them, indicating that the occupation of N-glycosylation sites contributes to interspecies variability in the B. jararaca complex.
33404253	6	11	theme	venom	1218:1222	arg1	analysis					1194:1201	The comparative glycoproteomic analysis	1163:1201	The comparative glycoproteomic analysis of B. alcatraz venom with B. jararaca and B. insularis	1163:1256	The comparative glycoproteomic analysis of B. alcatraz venom with B. jararaca and B. insularis indicated that there may be differences in the utilization of N-glycosylation motifs among their different toxin classes.
33404253	0	12	theme	Terminal	125:132	arg1	Labeling					149:156	Terminal Amine Isotopic Labeling	125:156	Terminal Amine Isotopic Labeling of Substrates	125:170	Venom Profiling of the Insular Species Bothrops alcatraz: Characterization of Proteome, Glycoproteome, and N-Terminome Using Terminal Amine Isotopic Labeling of Substrates.
33404253	6	13	theme	B.	1206:1207	arg1	venom					1218:1222	B. alcatraz venom	1206:1222	B. alcatraz venom	1206:1222	The comparative glycoproteomic analysis of B. alcatraz venom with B. jararaca and B. insularis indicated that there may be differences in the utilization of N-glycosylation motifs among their different toxin classes.
33404253	0	14	theme	Isotopic	140:147	arg1	Labeling					149:156	Terminal Amine Isotopic Labeling	125:156	Terminal Amine Isotopic Labeling of Substrates	125:170	Venom Profiling of the Insular Species Bothrops alcatraz: Characterization of Proteome, Glycoproteome, and N-Terminome Using Terminal Amine Isotopic Labeling of Substrates.
33404253	4	15	theme	interspecies	881:892	arg1	variability					894:904	interspecies variability	881:904	interspecies variability in the B. jararaca complex	881:931	Electrophoresis showed that B. alcatraz venom differs from B. jararaca and B. insularis; however, N-glycan removal revealed similarities between them, indicating that the occupation of N-glycosylation sites contributes to interspecies variability in the B. jararaca complex.
33404253	4	16	theme	alcatraz	690:697	arg1	venom					699:703	B. alcatraz venom	687:703	B. alcatraz venom	687:703	Electrophoresis showed that B. alcatraz venom differs from B. jararaca and B. insularis; however, N-glycan removal revealed similarities between them, indicating that the occupation of N-glycosylation sites contributes to interspecies variability in the B. jararaca complex.
33404253	5	17	theme	serine	1035:1040	arg1	proteinase					1042:1051	serine proteinase	1035:1051	serine proteinase	1035:1051	Metalloproteinase was the major toxin class identified in the B. alcatraz venom proteome followed by serine proteinase and C-type lectin, and overall, the adult B. alcatraz venom resembles that of B. jararaca juvenile specimens.
33404253	3	18	theme	B.	576:577	arg1	venom					588:592	B. alcatraz venom	576:592	B. alcatraz venom	576:592	Here, we characterized B. alcatraz venom using electrophoretic, proteomic, and glycoproteomic approaches.
33404253	8	19	theme	venom	1750:1754	arg1	transcriptomes					1762:1775	venom gland transcriptomes	1750:1775	venom gland transcriptomes	1750:1775	These findings underscore a low correlation between venom gland transcriptomes and proteomes and support the view that post-translational processes play a major role in shaping venom phenotypes.
33404253	6	20	theme	comparative	1167:1177	arg1	analysis					1194:1201	The comparative glycoproteomic analysis	1163:1201	The comparative glycoproteomic analysis of B. alcatraz venom with B. jararaca and B. insularis	1163:1256	The comparative glycoproteomic analysis of B. alcatraz venom with B. jararaca and B. insularis indicated that there may be differences in the utilization of N-glycosylation motifs among their different toxin classes.
33404253	5	21	theme	adult	1089:1093	arg1	venom					1107:1111	the adult B. alcatraz venom	1085:1111	the adult B. alcatraz venom	1085:1111	Metalloproteinase was the major toxin class identified in the B. alcatraz venom proteome followed by serine proteinase and C-type lectin, and overall, the adult B. alcatraz venom resembles that of B. jararaca juvenile specimens.
33404253	7	22	theme	B.	1679:1680	arg1	venom					1691:1695	B. alcatraz venom	1679:1695	B. alcatraz venom	1679:1695	Furthermore, we prospected for the first time the N-terminome of a snake venom using the terminal amine isotopic labeling of substrates (TAILS) approach and report the presence of ∼30% of N-termini corresponding to truncated toxin forms and ∼37% N-terminal sequences blocked by pyroglutamic acid in B. alcatraz venom.
33404253	6	23	theme	different	1355:1363	arg1	classes					1371:1377	their different toxin classes	1349:1377	their different toxin classes	1349:1377	The comparative glycoproteomic analysis of B. alcatraz venom with B. jararaca and B. insularis indicated that there may be differences in the utilization of N-glycosylation motifs among their different toxin classes.
33404253	7	24	dep	%	1563:1563	arg1	sequences					1637:1645	N-terminal sequences	1626:1645	N-terminal sequences	1626:1645	Furthermore, we prospected for the first time the N-terminome of a snake venom using the terminal amine isotopic labeling of substrates (TAILS) approach and report the presence of ∼30% of N-termini corresponding to truncated toxin forms and ∼37% N-terminal sequences blocked by pyroglutamic acid in B. alcatraz venom.
33404253	5	25	dep	B.	1131:1132	arg1	jararaca					1134:1141	jararaca	1134:1141	jararaca	1134:1141	Metalloproteinase was the major toxin class identified in the B. alcatraz venom proteome followed by serine proteinase and C-type lectin, and overall, the adult B. alcatraz venom resembles that of B. jararaca juvenile specimens.
33404253	6	26	theme	N-glycosylation	1320:1334	arg1	motifs					1336:1341	N-glycosylation motifs	1320:1341	N-glycosylation motifs	1320:1341	The comparative glycoproteomic analysis of B. alcatraz venom with B. jararaca and B. insularis indicated that there may be differences in the utilization of N-glycosylation motifs among their different toxin classes.
33404253	5	27	theme	toxin	966:970	arg1	class					972:976	the major toxin class	956:976	the major toxin class	956:976	Metalloproteinase was the major toxin class identified in the B. alcatraz venom proteome followed by serine proteinase and C-type lectin, and overall, the adult B. alcatraz venom resembles that of B. jararaca juvenile specimens.
33404253	2	28	theme	species	432:438	arg1	toxins					413:418	toxins	413:418	toxins of Bothrops species	413:438	We recently reported the identification of N-glycans attached to toxins of Bothrops species, showing similar compositions in venoms of the B. jararaca complex (B. jararaca, B. insularis, and B. alcatraz).
33404253	0	29	theme	Venom	0:4	arg1	Profiling					6:14	Venom Profiling	0:14	Venom Profiling of the Insular Species	0:37	Venom Profiling of the Insular Species Bothrops alcatraz: Characterization of Proteome, Glycoproteome, and N-Terminome Using Terminal Amine Isotopic Labeling of Substrates.
33404253	8	30	theme	post-translational	1817:1834	arg1	processes					1836:1844	post-translational processes	1817:1844	post-translational processes	1817:1844	These findings underscore a low correlation between venom gland transcriptomes and proteomes and support the view that post-translational processes play a major role in shaping venom phenotypes.
33404253	7	31	theme	%	1624:1624	arg1	presence					1548:1555	the presence	1544:1555	the presence of ∼30% of N-termini corresponding to truncated toxin forms and ∼37% N-terminal sequences blocked by pyroglutamic acid in B. alcatraz venom	1544:1695	Furthermore, we prospected for the first time the N-terminome of a snake venom using the terminal amine isotopic labeling of substrates (TAILS) approach and report the presence of ∼30% of N-termini corresponding to truncated toxin forms and ∼37% N-terminal sequences blocked by pyroglutamic acid in B. alcatraz venom.
33404253	2	32	dep	B.	487:488	arg1	jararaca					490:497	jararaca	490:497	jararaca	490:497	We recently reported the identification of N-glycans attached to toxins of Bothrops species, showing similar compositions in venoms of the B. jararaca complex (B. jararaca, B. insularis, and B. alcatraz).
33404253	2	33	attach	attached	401:408	arg2	N-glycans					391:399	N-glycans	391:399	N-glycans attached to toxins of Bothrops species	391:438	We recently reported the identification of N-glycans attached to toxins of Bothrops species, showing similar compositions in venoms of the B. jararaca complex (B. jararaca, B. insularis, and B. alcatraz).
33404253	2	33	attach	attached	401:408	arg1	toxins					413:418	toxins	413:418	toxins of Bothrops species	413:438	We recently reported the identification of N-glycans attached to toxins of Bothrops species, showing similar compositions in venoms of the B. jararaca complex (B. jararaca, B. insularis, and B. alcatraz).
33404253	4	34	dep	showed	675:680	arg1	revealed					774:781	revealed	774:781	revealed	774:781	Electrophoresis showed that B. alcatraz venom differs from B. jararaca and B. insularis; however, N-glycan removal revealed similarities between them, indicating that the occupation of N-glycosylation sites contributes to interspecies variability in the B. jararaca complex.
33404253	5	35	dep	B.	1095:1096	arg1	alcatraz					1098:1105	alcatraz	1098:1105	alcatraz	1098:1105	Metalloproteinase was the major toxin class identified in the B. alcatraz venom proteome followed by serine proteinase and C-type lectin, and overall, the adult B. alcatraz venom resembles that of B. jararaca juvenile specimens.
33404253	3	36	theme	electrophoretic	600:614	arg1	approaches					647:656	electrophoretic, proteomic, and glycoproteomic approaches	600:656	electrophoretic, proteomic, and glycoproteomic approaches	600:656	Here, we characterized B. alcatraz venom using electrophoretic, proteomic, and glycoproteomic approaches.
33404253	2	37	theme	N-glycans	391:399	arg1	identification					373:386	the identification	369:386	the identification of N-glycans attached to toxins of Bothrops species	369:438	We recently reported the identification of N-glycans attached to toxins of Bothrops species, showing similar compositions in venoms of the B. jararaca complex (B. jararaca, B. insularis, and B. alcatraz).
33404253	0	38	theme	Insular	23:29	arg1	Species					31:37	the Insular Species	19:37	the Insular Species	19:37	Venom Profiling of the Insular Species Bothrops alcatraz: Characterization of Proteome, Glycoproteome, and N-Terminome Using Terminal Amine Isotopic Labeling of Substrates.
33404253	2	39	dep	complex	499:505	arg1	alcatraz					542:549	B. alcatraz	539:549	B. alcatraz	539:549	We recently reported the identification of N-glycans attached to toxins of Bothrops species, showing similar compositions in venoms of the B. jararaca complex (B. jararaca, B. insularis, and B. alcatraz).
33404253	2	39	dep	complex	499:505	arg1	jararaca					511:518	B. jararaca	508:518	B. jararaca	508:518	We recently reported the identification of N-glycans attached to toxins of Bothrops species, showing similar compositions in venoms of the B. jararaca complex (B. jararaca, B. insularis, and B. alcatraz).
33404253	2	39	dep	complex	499:505	arg1	insularis					524:532	B. insularis	521:532	B. insularis	521:532	We recently reported the identification of N-glycans attached to toxins of Bothrops species, showing similar compositions in venoms of the B. jararaca complex (B. jararaca, B. insularis, and B. alcatraz).
33404253	0	40	dep	Bothrops	39:46	arg1	Characterization					58:73	Characterization	58:73	Characterization of Proteome, Glycoproteome, and N-Terminome Using Terminal Amine Isotopic Labeling of Substrates	58:170	Venom Profiling of the Insular Species Bothrops alcatraz: Characterization of Proteome, Glycoproteome, and N-Terminome Using Terminal Amine Isotopic Labeling of Substrates.
33404253	1	41	theme	occurrence	295:304	arg1	quality					324:330	the declining quality	310:330	the declining quality of its habitat	310:345	Bothrops alcatraz, a species endemic to Alcatrazes Islands, is regarded as critically endangered due to its small area of occurrence and the declining quality of its habitat.
33404253	1	41	theme	occurrence	295:304	arg1	area					287:290	its small area	277:290	its small area of occurrence	277:304	Bothrops alcatraz, a species endemic to Alcatrazes Islands, is regarded as critically endangered due to its small area of occurrence and the declining quality of its habitat.
33404253	7	42	theme	isotopic	1484:1491	arg1	labeling					1493:1500	the terminal amine isotopic labeling	1465:1500	the terminal amine isotopic labeling of substrates (TAILS) approach	1465:1531	Furthermore, we prospected for the first time the N-terminome of a snake venom using the terminal amine isotopic labeling of substrates (TAILS) approach and report the presence of ∼30% of N-termini corresponding to truncated toxin forms and ∼37% N-terminal sequences blocked by pyroglutamic acid in B. alcatraz venom.
33404253	7	43	theme	terminal	1469:1476	arg1	labeling					1493:1500	the terminal amine isotopic labeling	1465:1500	the terminal amine isotopic labeling of substrates (TAILS) approach	1465:1531	Furthermore, we prospected for the first time the N-terminome of a snake venom using the terminal amine isotopic labeling of substrates (TAILS) approach and report the presence of ∼30% of N-termini corresponding to truncated toxin forms and ∼37% N-terminal sequences blocked by pyroglutamic acid in B. alcatraz venom.
33404253	3	44	theme	proteomic	617:625	arg1	approaches					647:656	electrophoretic, proteomic, and glycoproteomic approaches	600:656	electrophoretic, proteomic, and glycoproteomic approaches	600:656	Here, we characterized B. alcatraz venom using electrophoretic, proteomic, and glycoproteomic approaches.
33404253	4	45	theme	N-glycan	757:764	arg1	removal					766:772	N-glycan removal	757:772	N-glycan removal	757:772	Electrophoresis showed that B. alcatraz venom differs from B. jararaca and B. insularis; however, N-glycan removal revealed similarities between them, indicating that the occupation of N-glycosylation sites contributes to interspecies variability in the B. jararaca complex.
33404253	6	46	theme	toxin	1365:1369	arg1	classes					1371:1377	their different toxin classes	1349:1377	their different toxin classes	1349:1377	The comparative glycoproteomic analysis of B. alcatraz venom with B. jararaca and B. insularis indicated that there may be differences in the utilization of N-glycosylation motifs among their different toxin classes.
33404253	5	47	theme	B.	1095:1096	arg1	venom					1107:1111	the adult B. alcatraz venom	1085:1111	the adult B. alcatraz venom	1085:1111	Metalloproteinase was the major toxin class identified in the B. alcatraz venom proteome followed by serine proteinase and C-type lectin, and overall, the adult B. alcatraz venom resembles that of B. jararaca juvenile specimens.
33404253	7	48	theme	snake	1447:1451	arg1	venom					1453:1457	a snake venom	1445:1457	a snake venom using the terminal amine isotopic labeling of substrates (TAILS) approach	1445:1531	Furthermore, we prospected for the first time the N-terminome of a snake venom using the terminal amine isotopic labeling of substrates (TAILS) approach and report the presence of ∼30% of N-termini corresponding to truncated toxin forms and ∼37% N-terminal sequences blocked by pyroglutamic acid in B. alcatraz venom.
33404253	8	49	theme	major	1853:1857	arg1	role					1859:1862	a major role	1851:1862	a major role	1851:1862	These findings underscore a low correlation between venom gland transcriptomes and proteomes and support the view that post-translational processes play a major role in shaping venom phenotypes.
33404253	5	50	theme	venom	1008:1012	arg1	proteome					1014:1021	the B. alcatraz venom proteome	992:1021	the B. alcatraz venom proteome followed by serine proteinase and C-type lectin	992:1069	Metalloproteinase was the major toxin class identified in the B. alcatraz venom proteome followed by serine proteinase and C-type lectin, and overall, the adult B. alcatraz venom resembles that of B. jararaca juvenile specimens.
33404253	6	51	from	differences	1286:1296	arg1	utilization					1305:1315	the utilization	1301:1315	the utilization of N-glycosylation motifs	1301:1341	The comparative glycoproteomic analysis of B. alcatraz venom with B. jararaca and B. insularis indicated that there may be differences in the utilization of N-glycosylation motifs among their different toxin classes.
33404253	8	52	theme	venom	1875:1879	arg1	phenotypes					1881:1890	shaping venom phenotypes	1867:1890	shaping venom phenotypes	1867:1890	These findings underscore a low correlation between venom gland transcriptomes and proteomes and support the view that post-translational processes play a major role in shaping venom phenotypes.
33404253	7	53	theme	toxin	1605:1609	arg1	forms					1611:1615	truncated toxin forms	1595:1615	truncated toxin forms	1595:1615	Furthermore, we prospected for the first time the N-terminome of a snake venom using the terminal amine isotopic labeling of substrates (TAILS) approach and report the presence of ∼30% of N-termini corresponding to truncated toxin forms and ∼37% N-terminal sequences blocked by pyroglutamic acid in B. alcatraz venom.
33404253	0	54	theme	Amine	134:138	arg1	Labeling					149:156	Terminal Amine Isotopic Labeling	125:156	Terminal Amine Isotopic Labeling of Substrates	125:170	Venom Profiling of the Insular Species Bothrops alcatraz: Characterization of Proteome, Glycoproteome, and N-Terminome Using Terminal Amine Isotopic Labeling of Substrates.
33404253	5	55	theme	C-type	1057:1062	arg1	lectin					1064:1069	C-type lectin	1057:1069	C-type lectin	1057:1069	Metalloproteinase was the major toxin class identified in the B. alcatraz venom proteome followed by serine proteinase and C-type lectin, and overall, the adult B. alcatraz venom resembles that of B. jararaca juvenile specimens.
33404253	1	56	theme	endemic	202:208	arg1	alcatraz					182:189	Bothrops alcatraz	173:189	Bothrops alcatraz	173:189	Bothrops alcatraz, a species endemic to Alcatrazes Islands, is regarded as critically endangered due to its small area of occurrence and the declining quality of its habitat.
33404253	1	56	theme	endemic	202:208	arg1	species					194:200	a species	192:200	a species endemic to Alcatrazes Islands	192:230	Bothrops alcatraz, a species endemic to Alcatrazes Islands, is regarded as critically endangered due to its small area of occurrence and the declining quality of its habitat.
33404253	7	57	theme	N-termini	1568:1576	arg1	%					1624:1624	∼37%	1621:1624	∼37%	1621:1624	Furthermore, we prospected for the first time the N-terminome of a snake venom using the terminal amine isotopic labeling of substrates (TAILS) approach and report the presence of ∼30% of N-termini corresponding to truncated toxin forms and ∼37% N-terminal sequences blocked by pyroglutamic acid in B. alcatraz venom.
33404253	7	57	theme	N-termini	1568:1576	arg1	N-termini					1568:1576	N-termini	1568:1576	N-termini corresponding to truncated toxin forms	1568:1615	Furthermore, we prospected for the first time the N-terminome of a snake venom using the terminal amine isotopic labeling of substrates (TAILS) approach and report the presence of ∼30% of N-termini corresponding to truncated toxin forms and ∼37% N-terminal sequences blocked by pyroglutamic acid in B. alcatraz venom.
33404253	7	57	theme	N-termini	1568:1576	arg1	%					1563:1563	∼30%	1560:1563	∼30% of N-termini corresponding to truncated toxin forms	1560:1615	Furthermore, we prospected for the first time the N-terminome of a snake venom using the terminal amine isotopic labeling of substrates (TAILS) approach and report the presence of ∼30% of N-termini corresponding to truncated toxin forms and ∼37% N-terminal sequences blocked by pyroglutamic acid in B. alcatraz venom.
33404253	6	58	theme	alcatraz	1209:1216	arg1	venom					1218:1222	B. alcatraz venom	1206:1222	B. alcatraz venom	1206:1222	The comparative glycoproteomic analysis of B. alcatraz venom with B. jararaca and B. insularis indicated that there may be differences in the utilization of N-glycosylation motifs among their different toxin classes.
33404253	5	59	dep	identified	978:987	arg1	class					972:976	the major toxin class	956:976	the major toxin class	956:976	Metalloproteinase was the major toxin class identified in the B. alcatraz venom proteome followed by serine proteinase and C-type lectin, and overall, the adult B. alcatraz venom resembles that of B. jararaca juvenile specimens.
33404253	4	60	dep	B.	913:914	arg1	jararaca					916:923	jararaca	916:923	jararaca	916:923	Electrophoresis showed that B. alcatraz venom differs from B. jararaca and B. insularis; however, N-glycan removal revealed similarities between them, indicating that the occupation of N-glycosylation sites contributes to interspecies variability in the B. jararaca complex.
33404253	4	61	from	variability	894:904	arg1	complex					925:931	the B. jararaca complex	909:931	the B. jararaca complex	909:931	Electrophoresis showed that B. alcatraz venom differs from B. jararaca and B. insularis; however, N-glycan removal revealed similarities between them, indicating that the occupation of N-glycosylation sites contributes to interspecies variability in the B. jararaca complex.
33404253	7	62	theme	%	1563:1563	arg1	presence					1548:1555	the presence	1544:1555	the presence of ∼30% of N-termini corresponding to truncated toxin forms and ∼37% N-terminal sequences blocked by pyroglutamic acid in B. alcatraz venom	1544:1695	Furthermore, we prospected for the first time the N-terminome of a snake venom using the terminal amine isotopic labeling of substrates (TAILS) approach and report the presence of ∼30% of N-termini corresponding to truncated toxin forms and ∼37% N-terminal sequences blocked by pyroglutamic acid in B. alcatraz venom.
33404253	4	63	theme	sites	860:864	arg1	occupation					830:839	the occupation	826:839	the occupation of N-glycosylation sites	826:864	Electrophoresis showed that B. alcatraz venom differs from B. jararaca and B. insularis; however, N-glycan removal revealed similarities between them, indicating that the occupation of N-glycosylation sites contributes to interspecies variability in the B. jararaca complex.
33404253	0	64	theme	Substrates	161:170	arg1	Labeling					149:156	Terminal Amine Isotopic Labeling	125:156	Terminal Amine Isotopic Labeling of Substrates	125:170	Venom Profiling of the Insular Species Bothrops alcatraz: Characterization of Proteome, Glycoproteome, and N-Terminome Using Terminal Amine Isotopic Labeling of Substrates.
33404253	8	65	theme	low	1726:1728	arg1	correlation					1730:1740	a low correlation	1724:1740	a low correlation between venom gland transcriptomes and proteomes	1724:1789	These findings underscore a low correlation between venom gland transcriptomes and proteomes and support the view that post-translational processes play a major role in shaping venom phenotypes.
33404253	6	66	theme	glycoproteomic	1179:1192	arg1	analysis					1194:1201	The comparative glycoproteomic analysis	1163:1201	The comparative glycoproteomic analysis of B. alcatraz venom with B. jararaca and B. insularis	1163:1256	The comparative glycoproteomic analysis of B. alcatraz venom with B. jararaca and B. insularis indicated that there may be differences in the utilization of N-glycosylation motifs among their different toxin classes.
33404253	4	67	theme	B.	687:688	arg1	venom					699:703	B. alcatraz venom	687:703	B. alcatraz venom	687:703	Electrophoresis showed that B. alcatraz venom differs from B. jararaca and B. insularis; however, N-glycan removal revealed similarities between them, indicating that the occupation of N-glycosylation sites contributes to interspecies variability in the B. jararaca complex.
33404253	0	68	theme	Proteome	78:85	arg1	Characterization					58:73	Characterization	58:73	Characterization of Proteome, Glycoproteome, and N-Terminome Using Terminal Amine Isotopic Labeling of Substrates	58:170	Venom Profiling of the Insular Species Bothrops alcatraz: Characterization of Proteome, Glycoproteome, and N-Terminome Using Terminal Amine Isotopic Labeling of Substrates.
33404253	1	69	theme	declining	314:322	arg1	quality					324:330	the declining quality	310:330	the declining quality of its habitat	310:345	Bothrops alcatraz, a species endemic to Alcatrazes Islands, is regarded as critically endangered due to its small area of occurrence and the declining quality of its habitat.
33404253	7	70	from	acid	1671:1674	arg1	venom					1691:1695	B. alcatraz venom	1679:1695	B. alcatraz venom	1679:1695	Furthermore, we prospected for the first time the N-terminome of a snake venom using the terminal amine isotopic labeling of substrates (TAILS) approach and report the presence of ∼30% of N-termini corresponding to truncated toxin forms and ∼37% N-terminal sequences blocked by pyroglutamic acid in B. alcatraz venom.
33404253	8	71	theme	gland	1756:1760	arg1	transcriptomes					1762:1775	venom gland transcriptomes	1750:1775	venom gland transcriptomes	1750:1775	These findings underscore a low correlation between venom gland transcriptomes and proteomes and support the view that post-translational processes play a major role in shaping venom phenotypes.
33404253	7	72	theme	alcatraz	1682:1689	arg1	venom					1691:1695	B. alcatraz venom	1679:1695	B. alcatraz venom	1679:1695	Furthermore, we prospected for the first time the N-terminome of a snake venom using the terminal amine isotopic labeling of substrates (TAILS) approach and report the presence of ∼30% of N-termini corresponding to truncated toxin forms and ∼37% N-terminal sequences blocked by pyroglutamic acid in B. alcatraz venom.
33404253	0	73	theme	Glycoproteome	88:100	arg1	Characterization					58:73	Characterization	58:73	Characterization of Proteome, Glycoproteome, and N-Terminome Using Terminal Amine Isotopic Labeling of Substrates	58:170	Venom Profiling of the Insular Species Bothrops alcatraz: Characterization of Proteome, Glycoproteome, and N-Terminome Using Terminal Amine Isotopic Labeling of Substrates.
33404253	6	74	with	analysis	1194:1201	arg1	jararaca					1232:1239	B. jararaca	1229:1239	B. jararaca	1229:1239	The comparative glycoproteomic analysis of B. alcatraz venom with B. jararaca and B. insularis indicated that there may be differences in the utilization of N-glycosylation motifs among their different toxin classes.
33404253	6	74	with	analysis	1194:1201	arg1	insularis					1248:1256	B. insularis	1245:1256	B. insularis	1245:1256	The comparative glycoproteomic analysis of B. alcatraz venom with B. jararaca and B. insularis indicated that there may be differences in the utilization of N-glycosylation motifs among their different toxin classes.
33404253	2	75	theme	complex	499:505	arg1	venoms					473:478	venoms	473:478	venoms of the B. jararaca complex (B. jararaca, B. insularis, and B. alcatraz)	473:550	We recently reported the identification of N-glycans attached to toxins of Bothrops species, showing similar compositions in venoms of the B. jararaca complex (B. jararaca, B. insularis, and B. alcatraz).
33404253	6	76	theme	motifs	1336:1341	arg1	utilization					1305:1315	the utilization	1301:1315	the utilization of N-glycosylation motifs	1301:1341	The comparative glycoproteomic analysis of B. alcatraz venom with B. jararaca and B. insularis indicated that there may be differences in the utilization of N-glycosylation motifs among their different toxin classes.
33404253	1	77	theme	habitat	339:345	arg1	quality					324:330	the declining quality	310:330	the declining quality of its habitat	310:345	Bothrops alcatraz, a species endemic to Alcatrazes Islands, is regarded as critically endangered due to its small area of occurrence and the declining quality of its habitat.
33404253	1	77	theme	habitat	339:345	arg1	area					287:290	its small area	277:290	its small area of occurrence	277:304	Bothrops alcatraz, a species endemic to Alcatrazes Islands, is regarded as critically endangered due to its small area of occurrence and the declining quality of its habitat.
33404253	5	78	theme	major	960:964	arg1	class					972:976	the major toxin class	956:976	the major toxin class	956:976	Metalloproteinase was the major toxin class identified in the B. alcatraz venom proteome followed by serine proteinase and C-type lectin, and overall, the adult B. alcatraz venom resembles that of B. jararaca juvenile specimens.
33404253	7	79	theme	pyroglutamic	1658:1669	arg1	acid					1671:1674	pyroglutamic acid	1658:1674	pyroglutamic acid in B. alcatraz venom	1658:1695	Furthermore, we prospected for the first time the N-terminome of a snake venom using the terminal amine isotopic labeling of substrates (TAILS) approach and report the presence of ∼30% of N-termini corresponding to truncated toxin forms and ∼37% N-terminal sequences blocked by pyroglutamic acid in B. alcatraz venom.
33404253	2	80	theme	B.	487:488	arg1	complex					499:505	the B. jararaca complex	483:505	the B. jararaca complex (B. jararaca, B. insularis, and B. alcatraz)	483:550	We recently reported the identification of N-glycans attached to toxins of Bothrops species, showing similar compositions in venoms of the B. jararaca complex (B. jararaca, B. insularis, and B. alcatraz).
33404253	2	81	from	compositions	457:468	arg1	venoms					473:478	venoms	473:478	venoms of the B. jararaca complex (B. jararaca, B. insularis, and B. alcatraz)	473:550	We recently reported the identification of N-glycans attached to toxins of Bothrops species, showing similar compositions in venoms of the B. jararaca complex (B. jararaca, B. insularis, and B. alcatraz).
33404253	2	82	theme	Bothrops	423:430	arg1	species					432:438	Bothrops species	423:438	Bothrops species	423:438	We recently reported the identification of N-glycans attached to toxins of Bothrops species, showing similar compositions in venoms of the B. jararaca complex (B. jararaca, B. insularis, and B. alcatraz).
33404253	5	83	theme	juvenile	1143:1150	arg1	specimens					1152:1160	B. jararaca juvenile specimens	1131:1160	B. jararaca juvenile specimens	1131:1160	Metalloproteinase was the major toxin class identified in the B. alcatraz venom proteome followed by serine proteinase and C-type lectin, and overall, the adult B. alcatraz venom resembles that of B. jararaca juvenile specimens.
33404253	7	84	theme	N-terminal	1626:1635	arg1	sequences					1637:1645	N-terminal sequences	1626:1645	N-terminal sequences	1626:1645	Furthermore, we prospected for the first time the N-terminome of a snake venom using the terminal amine isotopic labeling of substrates (TAILS) approach and report the presence of ∼30% of N-termini corresponding to truncated toxin forms and ∼37% N-terminal sequences blocked by pyroglutamic acid in B. alcatraz venom.
33404253	0	85	theme	Species	31:37	arg1	Profiling					6:14	Venom Profiling	0:14	Venom Profiling of the Insular Species	0:37	Venom Profiling of the Insular Species Bothrops alcatraz: Characterization of Proteome, Glycoproteome, and N-Terminome Using Terminal Amine Isotopic Labeling of Substrates.
33404253	1	86	theme	small	281:285	arg1	area					287:290	its small area	277:290	its small area of occurrence	277:304	Bothrops alcatraz, a species endemic to Alcatrazes Islands, is regarded as critically endangered due to its small area of occurrence and the declining quality of its habitat.
33404253	5	87	theme	B.	996:997	arg1	proteome					1014:1021	the B. alcatraz venom proteome	992:1021	the B. alcatraz venom proteome followed by serine proteinase and C-type lectin	992:1069	Metalloproteinase was the major toxin class identified in the B. alcatraz venom proteome followed by serine proteinase and C-type lectin, and overall, the adult B. alcatraz venom resembles that of B. jararaca juvenile specimens.
33404253	2	88	theme	similar	449:455	arg1	compositions					457:468	similar compositions	449:468	similar compositions in venoms of the B. jararaca complex (B. jararaca, B. insularis, and B. alcatraz)	449:550	We recently reported the identification of N-glycans attached to toxins of Bothrops species, showing similar compositions in venoms of the B. jararaca complex (B. jararaca, B. insularis, and B. alcatraz).
33404253	3	89	theme	alcatraz	579:586	arg1	venom					588:592	B. alcatraz venom	576:592	B. alcatraz venom	576:592	Here, we characterized B. alcatraz venom using electrophoretic, proteomic, and glycoproteomic approaches.
33404253	7	90	theme	substrates	1505:1514	arg1	labeling					1493:1500	the terminal amine isotopic labeling	1465:1500	the terminal amine isotopic labeling of substrates (TAILS) approach	1465:1531	Furthermore, we prospected for the first time the N-terminome of a snake venom using the terminal amine isotopic labeling of substrates (TAILS) approach and report the presence of ∼30% of N-termini corresponding to truncated toxin forms and ∼37% N-terminal sequences blocked by pyroglutamic acid in B. alcatraz venom.
33404253	6	91	gly	N-glycosylation	1320:1334	arg2	motifs					1336:1341	N-glycosylation motifs	1320:1341	N-glycosylation motifs	1320:1341	The comparative glycoproteomic analysis of B. alcatraz venom with B. jararaca and B. insularis indicated that there may be differences in the utilization of N-glycosylation motifs among their different toxin classes.
33404253	5	92	theme	B.	1131:1132	arg1	specimens					1152:1160	B. jararaca juvenile specimens	1131:1160	B. jararaca juvenile specimens	1131:1160	Metalloproteinase was the major toxin class identified in the B. alcatraz venom proteome followed by serine proteinase and C-type lectin, and overall, the adult B. alcatraz venom resembles that of B. jararaca juvenile specimens.
33404253	7	93	theme	labeling	1493:1500	arg1	approach					1524:1531	the terminal amine isotopic labeling of substrates (TAILS) approach	1465:1531	the terminal amine isotopic labeling of substrates (TAILS) approach	1465:1531	Furthermore, we prospected for the first time the N-terminome of a snake venom using the terminal amine isotopic labeling of substrates (TAILS) approach and report the presence of ∼30% of N-termini corresponding to truncated toxin forms and ∼37% N-terminal sequences blocked by pyroglutamic acid in B. alcatraz venom.
33404253	7	94	theme	amine	1478:1482	arg1	labeling					1493:1500	the terminal amine isotopic labeling	1465:1500	the terminal amine isotopic labeling of substrates (TAILS) approach	1465:1531	Furthermore, we prospected for the first time the N-terminome of a snake venom using the terminal amine isotopic labeling of substrates (TAILS) approach and report the presence of ∼30% of N-termini corresponding to truncated toxin forms and ∼37% N-terminal sequences blocked by pyroglutamic acid in B. alcatraz venom.
34463042	2	0	theme	spinal	534:539	arg1	tissue					550:555	spinal meninges tissue	534:555	spinal meninges tissue	534:555	In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges.
34463042	1	1	theme	alternative	195:205	arg1	source					207:212	an alternative source	192:212	an alternative source of regenerative biomaterials that preserve biochemical structure and matrix components of native tissues	192:317	Decellularization of extracellular matrices offers an alternative source of regenerative biomaterials that preserve biochemical structure and matrix components of native tissues.
34463042	1	2	theme	tissues	311:317	arg1	structure					269:277	biochemical structure	257:277	biochemical structure	257:277	Decellularization of extracellular matrices offers an alternative source of regenerative biomaterials that preserve biochemical structure and matrix components of native tissues.
34463042	1	2	theme	tissues	311:317	arg1	components					290:299	matrix components	283:299	matrix components	283:299	Decellularization of extracellular matrices offers an alternative source of regenerative biomaterials that preserve biochemical structure and matrix components of native tissues.
34463042	7	3	theme	vascular	1271:1278	arg1	branching					1280:1288	vascular branching	1271:1288	vascular branching	1271:1288	Furthermore, MeninGEL instructs human umbilical vein endothelial cells to form vascular branching.
34463042	2	4	theme	matrix	407:412	arg1	hydrogel					414:421	decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel	335:421	decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL)	335:432	In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges.
34463042	2	4	theme	matrix	407:412	arg1	MeninGEL					424:431	MeninGEL	424:431	MeninGEL	424:431	In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges.
34463042	0	5	theme	structure	120:128	arg1	formation					130:138	vascular structure formation	111:138	vascular structure formation	111:138	Decellularized spinal cord meninges extracellular matrix hydrogel that supports neurogenic differentiation and vascular structure formation.
34463042	2	6	link	-derived	384:391	arg1	hydrogel					414:421	decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel	335:421	decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL)	335:432	In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges.
34463042	2	6	link	-derived	384:391	arg1	MeninGEL					424:431	MeninGEL	424:431	MeninGEL	424:431	In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges.
34463042	2	7	theme	bovine	350:355	arg1	hydrogel					414:421	decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel	335:421	decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL)	335:432	In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges.
34463042	2	7	theme	bovine	350:355	arg1	MeninGEL					424:431	MeninGEL	424:431	MeninGEL	424:431	In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges.
34463042	6	8	theme	free	1112:1115	arg1	differentiation					1117:1131	growth-factor free differentiation	1098:1131	growth-factor free differentiation of human mesenchymal stem cells into neural-lineage cells	1098:1189	Moreover, it is evidenced with immuno/histochemistry and gene expression studies that the hydrogel induces growth-factor free differentiation of human mesenchymal stem cells into neural-lineage cells.
34463042	0	9	theme	vascular	111:118	arg1	formation					130:138	vascular structure formation	111:138	vascular structure formation	111:138	Decellularized spinal cord meninges extracellular matrix hydrogel that supports neurogenic differentiation and vascular structure formation.
34463042	5	10	contain	possesses	952:960	arg2	cytocompatibility					972:988	excellent cytocompatibility	962:988	excellent cytocompatibility	962:988	The MeninGEL possesses excellent cytocompatibility.
34463042	5	10	contain	possesses	952:960	arg1	MeninGEL					943:950	The MeninGEL	939:950	The MeninGEL	939:950	The MeninGEL possesses excellent cytocompatibility.
34463042	2	11	theme	decellularized	335:348	arg1	hydrogel					414:421	decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel	335:421	decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL)	335:432	In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges.
34463042	2	11	theme	decellularized	335:348	arg1	MeninGEL					424:431	MeninGEL	424:431	MeninGEL	424:431	In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges.
34463042	1	12	theme	matrices	176:183	arg1	Decellularization					141:157	Decellularization	141:157	Decellularization of extracellular matrices	141:183	Decellularization of extracellular matrices offers an alternative source of regenerative biomaterials that preserve biochemical structure and matrix components of native tissues.
34463042	8	13	theme	low	1323:1325	arg1	property					1352:1359	low batch-to-batch variation property	1323:1359	low batch-to-batch variation property	1323:1359	With its innate bioactivity and low batch-to-batch variation property, the MeninGEL has the potential to be an off-the-shelf product in nerve tissue regeneration and restoration.
34463042	7	14	theme	vein	1240:1243	arg1	cells					1257:1261	human umbilical vein endothelial cells	1224:1261	human umbilical vein endothelial cells	1224:1261	Furthermore, MeninGEL instructs human umbilical vein endothelial cells to form vascular branching.
34463042	8	15	theme	off-the-shelf	1402:1414	arg1	product					1416:1422	an off-the-shelf product	1399:1422	an off-the-shelf product	1399:1422	With its innate bioactivity and low batch-to-batch variation property, the MeninGEL has the potential to be an off-the-shelf product in nerve tissue regeneration and restoration.
34463042	4	16	theme	sweep	847:851	arg1	rheometry					853:861	temperature sweep rheometry	835:861	temperature sweep rheometry	835:861	Frequency and temperature sweep rheometry show that dSCM forms self-supporting hydrogel at physiological temperature.
34463042	5	17	theme	excellent	962:970	arg1	cytocompatibility					972:988	excellent cytocompatibility	962:988	excellent cytocompatibility	962:988	The MeninGEL possesses excellent cytocompatibility.
34463042	4	18	theme	physiological	912:924	arg1	temperature					926:936	physiological temperature	912:936	physiological temperature	912:936	Frequency and temperature sweep rheometry show that dSCM forms self-supporting hydrogel at physiological temperature.
34463042	4	19	theme	temperature	835:845	arg1	rheometry					853:861	temperature sweep rheometry	835:861	temperature sweep rheometry	835:861	Frequency and temperature sweep rheometry show that dSCM forms self-supporting hydrogel at physiological temperature.
34463042	1	20	theme	regenerative	217:228	arg1	biomaterials					230:241	regenerative biomaterials	217:241	regenerative biomaterials that preserve biochemical structure and matrix components of native tissues	217:317	Decellularization of extracellular matrices offers an alternative source of regenerative biomaterials that preserve biochemical structure and matrix components of native tissues.
34463042	0	21	theme	spinal	15:20	arg1	cord					22:25	Decellularized spinal cord	0:25	Decellularized spinal cord	0:25	Decellularized spinal cord meninges extracellular matrix hydrogel that supports neurogenic differentiation and vascular structure formation.
34463042	8	22	theme	batch-to-batch	1327:1340	arg1	property					1352:1359	low batch-to-batch variation property	1323:1359	low batch-to-batch variation property	1323:1359	With its innate bioactivity and low batch-to-batch variation property, the MeninGEL has the potential to be an off-the-shelf product in nerve tissue regeneration and restoration.
34463042	7	23	theme	human	1224:1228	arg1	cells					1257:1261	human umbilical vein endothelial cells	1224:1261	human umbilical vein endothelial cells	1224:1261	Furthermore, MeninGEL instructs human umbilical vein endothelial cells to form vascular branching.
34463042	2	24	theme	tissue	550:555	arg1	processing					520:529	physical, chemical, and enzymatic processing	486:529	physical, chemical, and enzymatic processing of spinal meninges tissue	486:555	In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges.
34463042	6	25	theme	cells	1159:1163	arg1	differentiation					1117:1131	growth-factor free differentiation	1098:1131	growth-factor free differentiation of human mesenchymal stem cells into neural-lineage cells	1098:1189	Moreover, it is evidenced with immuno/histochemistry and gene expression studies that the hydrogel induces growth-factor free differentiation of human mesenchymal stem cells into neural-lineage cells.
34463042	1	26	theme	biomaterials	230:241	arg1	source					207:212	an alternative source	192:212	an alternative source of regenerative biomaterials that preserve biochemical structure and matrix components of native tissues	192:317	Decellularization of extracellular matrices offers an alternative source of regenerative biomaterials that preserve biochemical structure and matrix components of native tissues.
34463042	0	27	theme	Decellularized	0:13	arg1	cord					22:25	Decellularized spinal cord	0:25	Decellularized spinal cord	0:25	Decellularized spinal cord meninges extracellular matrix hydrogel that supports neurogenic differentiation and vascular structure formation.
34463042	3	28	theme	MeninGEL	811:818	arg1	composition					796:806	the composition	792:806	the composition of MeninGEL	792:818	The success of decellularization is characterized by measuring the contents of residual DNA, glycosaminoglycans, and hydroxyproline, while a proteomics analysis is applied to reveal the composition of MeninGEL.
34463042	7	29	theme	umbilical	1230:1238	arg1	cells					1257:1261	human umbilical vein endothelial cells	1224:1261	human umbilical vein endothelial cells	1224:1261	Furthermore, MeninGEL instructs human umbilical vein endothelial cells to form vascular branching.
34463042	2	30	theme	meninges	369:376	arg1	hydrogel					414:421	decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel	335:421	decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL)	335:432	In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges.
34463042	2	30	theme	meninges	369:376	arg1	MeninGEL					424:431	MeninGEL	424:431	MeninGEL	424:431	In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges.
34463042	2	31	theme	physical	486:493	arg1	processing					520:529	physical, chemical, and enzymatic processing	486:529	physical, chemical, and enzymatic processing of spinal meninges tissue	486:555	In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges.
34463042	2	32	theme	cord	364:367	arg1	hydrogel					414:421	decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel	335:421	decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL)	335:432	In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges.
34463042	2	32	theme	cord	364:367	arg1	MeninGEL					424:431	MeninGEL	424:431	MeninGEL	424:431	In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges.
34463042	8	33	contain	has	1375:1377	arg1	MeninGEL					1366:1373	the MeninGEL	1362:1373	the MeninGEL	1362:1373	With its innate bioactivity and low batch-to-batch variation property, the MeninGEL has the potential to be an off-the-shelf product in nerve tissue regeneration and restoration.
34463042	8	33	contain	has	1375:1377	arg2	potential					1383:1391	the potential to be an off-the-shelf product in nerve tissue regeneration and restoration	1379:1467	the potential to be an off-the-shelf product in nerve tissue regeneration and restoration	1379:1467	With its innate bioactivity and low batch-to-batch variation property, the MeninGEL has the potential to be an off-the-shelf product in nerve tissue regeneration and restoration.
34463042	3	34	theme	decellularization	625:641	arg1	success					614:620	The success	610:620	The success of decellularization	610:641	The success of decellularization is characterized by measuring the contents of residual DNA, glycosaminoglycans, and hydroxyproline, while a proteomics analysis is applied to reveal the composition of MeninGEL.
34463042	2	35	theme	meninges	600:607	arg1	structure					587:595	the biochemical structure	571:595	the biochemical structure of meninges	571:607	In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges.
34463042	3	36	theme	glycosaminoglycans	703:720	arg1	contents					677:684	the contents	673:684	the contents of residual DNA, glycosaminoglycans, and hydroxyproline	673:740	The success of decellularization is characterized by measuring the contents of residual DNA, glycosaminoglycans, and hydroxyproline, while a proteomics analysis is applied to reveal the composition of MeninGEL.
34463042	2	37	theme	spinal	357:362	arg1	hydrogel					414:421	decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel	335:421	decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL)	335:432	In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges.
34463042	2	37	theme	spinal	357:362	arg1	MeninGEL					424:431	MeninGEL	424:431	MeninGEL	424:431	In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges.
34463042	1	38	theme	biochemical	257:267	arg1	structure					269:277	biochemical structure	257:277	biochemical structure	257:277	Decellularization of extracellular matrices offers an alternative source of regenerative biomaterials that preserve biochemical structure and matrix components of native tissues.
34463042	0	39	theme	matrix	50:55	arg1	hydrogel					57:64	extracellular matrix hydrogel	36:64	extracellular matrix hydrogel that supports neurogenic differentiation and vascular structure formation	36:138	Decellularized spinal cord meninges extracellular matrix hydrogel that supports neurogenic differentiation and vascular structure formation.
34463042	8	40	theme	tissue	1433:1438	arg1	regeneration					1440:1451	nerve tissue regeneration	1427:1451	nerve tissue regeneration	1427:1451	With its innate bioactivity and low batch-to-batch variation property, the MeninGEL has the potential to be an off-the-shelf product in nerve tissue regeneration and restoration.
34463042	8	41	theme	nerve	1427:1431	arg1	regeneration					1440:1451	nerve tissue regeneration	1427:1451	nerve tissue regeneration	1427:1451	With its innate bioactivity and low batch-to-batch variation property, the MeninGEL has the potential to be an off-the-shelf product in nerve tissue regeneration and restoration.
34463042	4	42	theme	self-supporting	884:898	arg1	hydrogel					900:907	self-supporting hydrogel	884:907	self-supporting hydrogel	884:907	Frequency and temperature sweep rheometry show that dSCM forms self-supporting hydrogel at physiological temperature.
34463042	6	43	theme	neural-lineage	1170:1183	arg1	cells					1185:1189	neural-lineage cells	1170:1189	neural-lineage cells	1170:1189	Moreover, it is evidenced with immuno/histochemistry and gene expression studies that the hydrogel induces growth-factor free differentiation of human mesenchymal stem cells into neural-lineage cells.
34463042	0	44	theme	extracellular	36:48	arg1	hydrogel					57:64	extracellular matrix hydrogel	36:64	extracellular matrix hydrogel that supports neurogenic differentiation and vascular structure formation	36:138	Decellularized spinal cord meninges extracellular matrix hydrogel that supports neurogenic differentiation and vascular structure formation.
34463042	2	45	theme	enzymatic	510:518	arg1	processing					520:529	physical, chemical, and enzymatic processing	486:529	physical, chemical, and enzymatic processing of spinal meninges tissue	486:555	In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges.
34463042	3	46	theme	DNA	698:700	arg1	contents					677:684	the contents	673:684	the contents of residual DNA, glycosaminoglycans, and hydroxyproline	673:740	The success of decellularization is characterized by measuring the contents of residual DNA, glycosaminoglycans, and hydroxyproline, while a proteomics analysis is applied to reveal the composition of MeninGEL.
34463042	8	47	theme	variation	1342:1350	arg1	property					1352:1359	low batch-to-batch variation property	1323:1359	low batch-to-batch variation property	1323:1359	With its innate bioactivity and low batch-to-batch variation property, the MeninGEL has the potential to be an off-the-shelf product in nerve tissue regeneration and restoration.
34463042	2	48	theme	biochemical	575:585	arg1	structure					587:595	the biochemical structure	571:595	the biochemical structure of meninges	571:607	In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges.
34463042	2	49	theme	extracellular	393:405	arg1	hydrogel					414:421	decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel	335:421	decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL)	335:432	In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges.
34463042	2	49	theme	extracellular	393:405	arg1	MeninGEL					424:431	MeninGEL	424:431	MeninGEL	424:431	In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges.
34463042	3	50	theme	proteomics	751:760	arg1	analysis					762:769	a proteomics analysis	749:769	a proteomics analysis	749:769	The success of decellularization is characterized by measuring the contents of residual DNA, glycosaminoglycans, and hydroxyproline, while a proteomics analysis is applied to reveal the composition of MeninGEL.
34463042	6	51	theme	gene	1048:1051	arg1	studies					1064:1070	gene expression studies	1048:1070	gene expression studies	1048:1070	Moreover, it is evidenced with immuno/histochemistry and gene expression studies that the hydrogel induces growth-factor free differentiation of human mesenchymal stem cells into neural-lineage cells.
34463042	7	52	theme	endothelial	1245:1255	arg1	cells					1257:1261	human umbilical vein endothelial cells	1224:1261	human umbilical vein endothelial cells	1224:1261	Furthermore, MeninGEL instructs human umbilical vein endothelial cells to form vascular branching.
34463042	2	53	theme	-derived	384:391	arg1	hydrogel					414:421	decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel	335:421	decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL)	335:432	In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges.
34463042	2	53	theme	-derived	384:391	arg1	MeninGEL					424:431	MeninGEL	424:431	MeninGEL	424:431	In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges.
34463042	8	54	theme	innate	1300:1305	arg1	bioactivity					1307:1317	its innate bioactivity	1296:1317	its innate bioactivity	1296:1317	With its innate bioactivity and low batch-to-batch variation property, the MeninGEL has the potential to be an off-the-shelf product in nerve tissue regeneration and restoration.
34463042	1	55	theme	extracellular	162:174	arg1	matrices					176:183	extracellular matrices	162:183	extracellular matrices	162:183	Decellularization of extracellular matrices offers an alternative source of regenerative biomaterials that preserve biochemical structure and matrix components of native tissues.
34463042	1	56	theme	matrix	283:288	arg1	components					290:299	matrix components	283:299	matrix components	283:299	Decellularization of extracellular matrices offers an alternative source of regenerative biomaterials that preserve biochemical structure and matrix components of native tissues.
34463042	3	57	theme	residual	689:696	arg1	DNA					698:700	residual DNA	689:700	residual DNA	689:700	The success of decellularization is characterized by measuring the contents of residual DNA, glycosaminoglycans, and hydroxyproline, while a proteomics analysis is applied to reveal the composition of MeninGEL.
34463042	2	58	theme	dSCM	379:382	arg1	hydrogel					414:421	decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel	335:421	decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL)	335:432	In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges.
34463042	2	58	theme	dSCM	379:382	arg1	MeninGEL					424:431	MeninGEL	424:431	MeninGEL	424:431	In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges.
34463042	6	59	theme	stem	1154:1157	arg1	cells					1159:1163	human mesenchymal stem cells	1136:1163	human mesenchymal stem cells	1136:1163	Moreover, it is evidenced with immuno/histochemistry and gene expression studies that the hydrogel induces growth-factor free differentiation of human mesenchymal stem cells into neural-lineage cells.
34463042	0	60	theme	neurogenic	80:89	arg1	differentiation					91:105	neurogenic differentiation	80:105	neurogenic differentiation	80:105	Decellularized spinal cord meninges extracellular matrix hydrogel that supports neurogenic differentiation and vascular structure formation.
34463042	3	61	theme	hydroxyproline	727:740	arg1	contents					677:684	the contents	673:684	the contents of residual DNA, glycosaminoglycans, and hydroxyproline	673:740	The success of decellularization is characterized by measuring the contents of residual DNA, glycosaminoglycans, and hydroxyproline, while a proteomics analysis is applied to reveal the composition of MeninGEL.
34463042	6	62	theme	mesenchymal	1142:1152	arg1	cells					1159:1163	human mesenchymal stem cells	1136:1163	human mesenchymal stem cells	1136:1163	Moreover, it is evidenced with immuno/histochemistry and gene expression studies that the hydrogel induces growth-factor free differentiation of human mesenchymal stem cells into neural-lineage cells.
34463042	2	63	theme	meninges	541:548	arg1	tissue					550:555	spinal meninges tissue	534:555	spinal meninges tissue	534:555	In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges.
34463042	6	64	theme	human	1136:1140	arg1	cells					1159:1163	human mesenchymal stem cells	1136:1163	human mesenchymal stem cells	1136:1163	Moreover, it is evidenced with immuno/histochemistry and gene expression studies that the hydrogel induces growth-factor free differentiation of human mesenchymal stem cells into neural-lineage cells.
34463042	8	65	from	product	1416:1422	arg1	restoration					1457:1467	restoration	1457:1467	restoration	1457:1467	With its innate bioactivity and low batch-to-batch variation property, the MeninGEL has the potential to be an off-the-shelf product in nerve tissue regeneration and restoration.
34463042	8	65	from	product	1416:1422	arg1	regeneration					1440:1451	nerve tissue regeneration	1427:1451	nerve tissue regeneration	1427:1451	With its innate bioactivity and low batch-to-batch variation property, the MeninGEL has the potential to be an off-the-shelf product in nerve tissue regeneration and restoration.
34463042	2	66	theme	chemical	496:503	arg1	processing					520:529	physical, chemical, and enzymatic processing	486:529	physical, chemical, and enzymatic processing of spinal meninges tissue	486:555	In this study, decellularized bovine spinal cord meninges (dSCM)-derived extracellular matrix hydrogel (MeninGEL) is fabricated by employing a protocol that involves physical, chemical, and enzymatic processing of spinal meninges tissue and preserves the biochemical structure of meninges.
34463042	6	67	theme	expression	1053:1062	arg1	studies					1064:1070	gene expression studies	1048:1070	gene expression studies	1048:1070	Moreover, it is evidenced with immuno/histochemistry and gene expression studies that the hydrogel induces growth-factor free differentiation of human mesenchymal stem cells into neural-lineage cells.
34463042	1	68	theme	native	304:309	arg1	tissues					311:317	native tissues	304:317	native tissues	304:317	Decellularization of extracellular matrices offers an alternative source of regenerative biomaterials that preserve biochemical structure and matrix components of native tissues.
32751149	5	0	theme	lactate	764:770	arg1	production					772:781	lactate production	764:781	lactate production (leading to pH decrease)	764:806	RESULTS BMOS alone significantly induced acetate and lactate production (leading to pH decrease) and stimulated bifidobacterial growth in 10 donors.
32751149	8	1	theme	activity	1142:1149	arg1	level					1151:1155	the microbiota activity level	1127:1155	the microbiota activity level	1127:1155	This was also observed at the microbiota activity level, especially in a donor containing low initial levels of bifidobacteria.
32751149	4	2	theme	three-month-old	676:690	arg1	microbiota					699:708	different three-month-old infant microbiota	666:708	different three-month-old infant microbiota	666:708	METHOD Short-term fecal batch fermentations (48 h) were used to assess the microbial composition and activity modulated by BMOS alone, B. lactis grown on BMOS or dextrose alone, or their combinations on different three-month-old infant microbiota.
32751149	9	3	theme	synbiotic	1408:1416	arg1	effect					1418:1423	the strong synbiotic effect	1397:1423	the strong synbiotic effect of BMOS and B. lactis on the infant gut microbiota	1397:1474	In this donor, preculturing B. lactis with BMOS strengthened further the early modulation of microbiota activity observed after 6 h. CONCLUSION This study demonstrated the strong synbiotic effect of BMOS and B. lactis on the infant gut microbiota, and suggests a strategy to improve its effectiveness in an otherwise low-Bifidobacterium microbiota.
32751149	0	4	theme	Oligosaccharides	79:94	arg1	Effect					10:15	Synbiotic Effect	0:15	Synbiotic Effect of Bifidobacterium lactis CNCM I-3446 and Bovine Milk-Derived Oligosaccharides on Infant Gut Microbiota.	0:120	Synbiotic Effect of Bifidobacterium lactis CNCM I-3446 and Bovine Milk-Derived Oligosaccharides on Infant Gut Microbiota.
32751149	0	5	from	Effect	10:15	arg1	Microbiota					110:119	Infant Gut Microbiota	99:119	Infant Gut Microbiota	99:119	Synbiotic Effect of Bifidobacterium lactis CNCM I-3446 and Bovine Milk-Derived Oligosaccharides on Infant Gut Microbiota.
32751149	7	6	theme	strong	1076:1081	arg1	effect					1093:1098	a strong synbiotic effect	1074:1098	a strong synbiotic effect	1074:1098	BMOS further enhanced this engraftment, suggesting a strong synbiotic effect.
32751149	1	7	theme	Bifidobacterium	168:182	arg1	ssp					193:195	Bifidobacterium animalis ssp	168:195	Bifidobacterium animalis ssp	168:195	BACKGROUND This study evaluated the impact of Bifidobacterium animalis ssp.
32751149	0	8	theme	Gut	106:108	arg1	Microbiota					110:119	Infant Gut Microbiota	99:119	Infant Gut Microbiota	99:119	Synbiotic Effect of Bifidobacterium lactis CNCM I-3446 and Bovine Milk-Derived Oligosaccharides on Infant Gut Microbiota.
32751149	6	9	theme	different	892:900	arg1	donors					902:907	two different donors	888:907	two different donors	888:907	A further in-depth study on two different donors proved B. lactis ability to colonize the infant microbiota, regardless of the competitiveness of the environment.
32751149	6	10	dep	B.	916:917	arg1	lactis					919:924	lactis	919:924	lactis	919:924	A further in-depth study on two different donors proved B. lactis ability to colonize the infant microbiota, regardless of the competitiveness of the environment.
32751149	0	11	theme	Infant	99:104	arg1	Microbiota					110:119	Infant Gut Microbiota	99:119	Infant Gut Microbiota	99:119	Synbiotic Effect of Bifidobacterium lactis CNCM I-3446 and Bovine Milk-Derived Oligosaccharides on Infant Gut Microbiota.
32751149	4	12	theme	batch	487:491	arg1	fermentations					493:505	METHOD Short-term fecal batch fermentations	463:505	METHOD Short-term fecal batch fermentations (48 h)	463:512	METHOD Short-term fecal batch fermentations (48 h) were used to assess the microbial composition and activity modulated by BMOS alone, B. lactis grown on BMOS or dextrose alone, or their combinations on different three-month-old infant microbiota.
32751149	4	12	theme	batch	487:491	arg1	h					511:511	48 h	508:511	48 h	508:511	METHOD Short-term fecal batch fermentations (48 h) were used to assess the microbial composition and activity modulated by BMOS alone, B. lactis grown on BMOS or dextrose alone, or their combinations on different three-month-old infant microbiota.
32751149	2	13	theme	gut	294:296	arg1	microbiota					298:307	infant gut microbiota	287:307	infant gut microbiota in vitro	287:316	lactis CNCM I-3446, Bovine Milk-derived OligoSaccharides (BMOS) and their combination on infant gut microbiota in vitro.
32751149	2	14	from	OligoSaccharides	238:253	arg1	microbiota					298:307	infant gut microbiota	287:307	infant gut microbiota in vitro	287:316	lactis CNCM I-3446, Bovine Milk-derived OligoSaccharides (BMOS) and their combination on infant gut microbiota in vitro.
32751149	9	15	theme	BMOS	1428:1431	arg1	effect					1418:1423	the strong synbiotic effect	1397:1423	the strong synbiotic effect of BMOS and B. lactis on the infant gut microbiota	1397:1474	In this donor, preculturing B. lactis with BMOS strengthened further the early modulation of microbiota activity observed after 6 h. CONCLUSION This study demonstrated the strong synbiotic effect of BMOS and B. lactis on the infant gut microbiota, and suggests a strategy to improve its effectiveness in an otherwise low-Bifidobacterium microbiota.
32751149	4	16	theme	fecal	481:485	arg1	fermentations					493:505	METHOD Short-term fecal batch fermentations	463:505	METHOD Short-term fecal batch fermentations (48 h)	463:512	METHOD Short-term fecal batch fermentations (48 h) were used to assess the microbial composition and activity modulated by BMOS alone, B. lactis grown on BMOS or dextrose alone, or their combinations on different three-month-old infant microbiota.
32751149	4	16	theme	fecal	481:485	arg1	h					511:511	48 h	508:511	48 h	508:511	METHOD Short-term fecal batch fermentations (48 h) were used to assess the microbial composition and activity modulated by BMOS alone, B. lactis grown on BMOS or dextrose alone, or their combinations on different three-month-old infant microbiota.
32751149	1	17	dep	BACKGROUND	122:131	arg1	evaluated					144:152	evaluated	144:152	evaluated the impact of Bifidobacterium animalis ssp	144:195	BACKGROUND This study evaluated the impact of Bifidobacterium animalis ssp.
32751149	1	18	theme	animalis	184:191	arg1	ssp					193:195	Bifidobacterium animalis ssp	168:195	Bifidobacterium animalis ssp	168:195	BACKGROUND This study evaluated the impact of Bifidobacterium animalis ssp.
32751149	5	19	theme	pH	795:796	arg1	decrease					798:805	pH decrease	795:805	pH decrease	795:805	RESULTS BMOS alone significantly induced acetate and lactate production (leading to pH decrease) and stimulated bifidobacterial growth in 10 donors.
32751149	4	20	dep	composition	548:558	arg1	the					534:536	the	534:536	the	534:536	METHOD Short-term fecal batch fermentations (48 h) were used to assess the microbial composition and activity modulated by BMOS alone, B. lactis grown on BMOS or dextrose alone, or their combinations on different three-month-old infant microbiota.
32751149	6	21	theme	environment	1010:1020	arg1	competitiveness					987:1001	the competitiveness	983:1001	the competitiveness of the environment	983:1020	A further in-depth study on two different donors proved B. lactis ability to colonize the infant microbiota, regardless of the competitiveness of the environment.
32751149	1	22	theme	ssp	193:195	arg1	impact					158:163	the impact	154:163	the impact of Bifidobacterium animalis ssp	154:195	BACKGROUND This study evaluated the impact of Bifidobacterium animalis ssp.
32751149	6	23	theme	in-depth	870:877	arg1	study					879:883	A further in-depth study	860:883	A further in-depth study on two different donors	860:907	A further in-depth study on two different donors proved B. lactis ability to colonize the infant microbiota, regardless of the competitiveness of the environment.
32751149	4	24	used	used	519:522	arg2	h					511:511	48 h	508:511	48 h	508:511	METHOD Short-term fecal batch fermentations (48 h) were used to assess the microbial composition and activity modulated by BMOS alone, B. lactis grown on BMOS or dextrose alone, or their combinations on different three-month-old infant microbiota.
32751149	4	24	used	used	519:522	arg2	fermentations					493:505	METHOD Short-term fecal batch fermentations	463:505	METHOD Short-term fecal batch fermentations (48 h)	463:512	METHOD Short-term fecal batch fermentations (48 h) were used to assess the microbial composition and activity modulated by BMOS alone, B. lactis grown on BMOS or dextrose alone, or their combinations on different three-month-old infant microbiota.
32751149	9	25	theme	low-Bifidobacterium	1546:1564	arg1	microbiota					1566:1575	an otherwise low-Bifidobacterium microbiota	1533:1575	an otherwise low-Bifidobacterium microbiota	1533:1575	In this donor, preculturing B. lactis with BMOS strengthened further the early modulation of microbiota activity observed after 6 h. CONCLUSION This study demonstrated the strong synbiotic effect of BMOS and B. lactis on the infant gut microbiota, and suggests a strategy to improve its effectiveness in an otherwise low-Bifidobacterium microbiota.
32751149	9	26	theme	early	1302:1306	arg1	modulation					1308:1317	the early modulation	1298:1317	the early modulation of microbiota activity observed after 6 h. CONCLUSION This study demonstrated the strong synbiotic effect of BMOS and B. lactis on the infant gut microbiota	1298:1474	In this donor, preculturing B. lactis with BMOS strengthened further the early modulation of microbiota activity observed after 6 h. CONCLUSION This study demonstrated the strong synbiotic effect of BMOS and B. lactis on the infant gut microbiota, and suggests a strategy to improve its effectiveness in an otherwise low-Bifidobacterium microbiota.
32751149	3	27	theme	potential	421:429	arg1	effects					441:447	their potential synbiotic effects	415:447	their potential synbiotic effects	415:447	In addition, a novel strategy consisting of preculturing B. lactis with BMOS to further enhance their potential synbiotic effects was assessed.
32751149	0	28	theme	Synbiotic	0:8	arg1	Effect					10:15	Synbiotic Effect	0:15	Synbiotic Effect of Bifidobacterium lactis CNCM I-3446 and Bovine Milk-Derived Oligosaccharides on Infant Gut Microbiota.	0:120	Synbiotic Effect of Bifidobacterium lactis CNCM I-3446 and Bovine Milk-Derived Oligosaccharides on Infant Gut Microbiota.
32751149	9	29	theme	microbiota	1322:1331	arg1	activity					1333:1340	microbiota activity	1322:1340	microbiota activity observed after 6 h. CONCLUSION	1322:1371	In this donor, preculturing B. lactis with BMOS strengthened further the early modulation of microbiota activity observed after 6 h. CONCLUSION This study demonstrated the strong synbiotic effect of BMOS and B. lactis on the infant gut microbiota, and suggests a strategy to improve its effectiveness in an otherwise low-Bifidobacterium microbiota.
32751149	7	30	theme	synbiotic	1083:1091	arg1	effect					1093:1098	a strong synbiotic effect	1074:1098	a strong synbiotic effect	1074:1098	BMOS further enhanced this engraftment, suggesting a strong synbiotic effect.
32751149	0	31	theme	Bifidobacterium	20:34	arg1	I-3446					48:53	Bifidobacterium lactis CNCM I-3446	20:53	Bifidobacterium lactis CNCM I-3446	20:53	Synbiotic Effect of Bifidobacterium lactis CNCM I-3446 and Bovine Milk-Derived Oligosaccharides on Infant Gut Microbiota.
32751149	4	32	theme	microbial	538:546	arg1	composition					548:558	microbial composition	538:558	microbial composition	538:558	METHOD Short-term fecal batch fermentations (48 h) were used to assess the microbial composition and activity modulated by BMOS alone, B. lactis grown on BMOS or dextrose alone, or their combinations on different three-month-old infant microbiota.
32751149	4	33	theme	METHOD	463:468	arg1	fermentations					493:505	METHOD Short-term fecal batch fermentations	463:505	METHOD Short-term fecal batch fermentations (48 h)	463:512	METHOD Short-term fecal batch fermentations (48 h) were used to assess the microbial composition and activity modulated by BMOS alone, B. lactis grown on BMOS or dextrose alone, or their combinations on different three-month-old infant microbiota.
32751149	4	33	theme	METHOD	463:468	arg1	h					511:511	48 h	508:511	48 h	508:511	METHOD Short-term fecal batch fermentations (48 h) were used to assess the microbial composition and activity modulated by BMOS alone, B. lactis grown on BMOS or dextrose alone, or their combinations on different three-month-old infant microbiota.
32751149	9	34	dep	B.	1437:1438	arg1	lactis					1440:1445	B. lactis	1437:1445	B. lactis	1437:1445	In this donor, preculturing B. lactis with BMOS strengthened further the early modulation of microbiota activity observed after 6 h. CONCLUSION This study demonstrated the strong synbiotic effect of BMOS and B. lactis on the infant gut microbiota, and suggests a strategy to improve its effectiveness in an otherwise low-Bifidobacterium microbiota.
32751149	8	35	located	observed	1115:1122	arg2	This					1101:1104	This	1101:1104	This	1101:1104	This was also observed at the microbiota activity level, especially in a donor containing low initial levels of bifidobacteria.
32751149	8	35	located	observed	1115:1122	arg1	level					1151:1155	the microbiota activity level	1127:1155	the microbiota activity level	1127:1155	This was also observed at the microbiota activity level, especially in a donor containing low initial levels of bifidobacteria.
32751149	8	35	located	observed	1115:1122	arg1	donor					1174:1178	a donor	1172:1178	a donor containing low initial levels of bifidobacteria	1172:1226	This was also observed at the microbiota activity level, especially in a donor containing low initial levels of bifidobacteria.
32751149	5	36	theme	bifidobacterial	823:837	arg1	growth					839:844	bifidobacterial growth	823:844	bifidobacterial growth in 10 donors	823:857	RESULTS BMOS alone significantly induced acetate and lactate production (leading to pH decrease) and stimulated bifidobacterial growth in 10 donors.
32751149	6	37	theme	infant	950:955	arg1	microbiota					957:966	the infant microbiota	946:966	the infant microbiota	946:966	A further in-depth study on two different donors proved B. lactis ability to colonize the infant microbiota, regardless of the competitiveness of the environment.
32751149	2	38	theme	infant	287:292	arg1	microbiota					298:307	infant gut microbiota	287:307	infant gut microbiota in vitro	287:316	lactis CNCM I-3446, Bovine Milk-derived OligoSaccharides (BMOS) and their combination on infant gut microbiota in vitro.
32751149	6	39	theme	further	862:868	arg1	study					879:883	A further in-depth study	860:883	A further in-depth study on two different donors	860:907	A further in-depth study on two different donors proved B. lactis ability to colonize the infant microbiota, regardless of the competitiveness of the environment.
32751149	4	40	theme	different	666:674	arg1	microbiota					699:708	different three-month-old infant microbiota	666:708	different three-month-old infant microbiota	666:708	METHOD Short-term fecal batch fermentations (48 h) were used to assess the microbial composition and activity modulated by BMOS alone, B. lactis grown on BMOS or dextrose alone, or their combinations on different three-month-old infant microbiota.
32751149	2	41	from	combination	272:282	arg1	microbiota					298:307	infant gut microbiota	287:307	infant gut microbiota in vitro	287:316	lactis CNCM I-3446, Bovine Milk-derived OligoSaccharides (BMOS) and their combination on infant gut microbiota in vitro.
32751149	9	42	theme	infant	1454:1459	arg1	microbiota					1465:1474	the infant gut microbiota	1450:1474	the infant gut microbiota	1450:1474	In this donor, preculturing B. lactis with BMOS strengthened further the early modulation of microbiota activity observed after 6 h. CONCLUSION This study demonstrated the strong synbiotic effect of BMOS and B. lactis on the infant gut microbiota, and suggests a strategy to improve its effectiveness in an otherwise low-Bifidobacterium microbiota.
32751149	0	43	theme	CNCM	43:46	arg1	I-3446					48:53	Bifidobacterium lactis CNCM I-3446	20:53	Bifidobacterium lactis CNCM I-3446	20:53	Synbiotic Effect of Bifidobacterium lactis CNCM I-3446 and Bovine Milk-Derived Oligosaccharides on Infant Gut Microbiota.
32751149	9	44	from	effect	1418:1423	arg1	microbiota					1465:1474	the infant gut microbiota	1450:1474	the infant gut microbiota	1450:1474	In this donor, preculturing B. lactis with BMOS strengthened further the early modulation of microbiota activity observed after 6 h. CONCLUSION This study demonstrated the strong synbiotic effect of BMOS and B. lactis on the infant gut microbiota, and suggests a strategy to improve its effectiveness in an otherwise low-Bifidobacterium microbiota.
32751149	3	45	dep	potential	421:429	arg1	synbiotic					431:439	synbiotic	431:439	synbiotic	431:439	In addition, a novel strategy consisting of preculturing B. lactis with BMOS to further enhance their potential synbiotic effects was assessed.
32751149	9	46	theme	gut	1461:1463	arg1	microbiota					1465:1474	the infant gut microbiota	1450:1474	the infant gut microbiota	1450:1474	In this donor, preculturing B. lactis with BMOS strengthened further the early modulation of microbiota activity observed after 6 h. CONCLUSION This study demonstrated the strong synbiotic effect of BMOS and B. lactis on the infant gut microbiota, and suggests a strategy to improve its effectiveness in an otherwise low-Bifidobacterium microbiota.
32751149	0	47	theme	lactis	36:41	arg1	I-3446					48:53	Bifidobacterium lactis CNCM I-3446	20:53	Bifidobacterium lactis CNCM I-3446	20:53	Synbiotic Effect of Bifidobacterium lactis CNCM I-3446 and Bovine Milk-Derived Oligosaccharides on Infant Gut Microbiota.
32751149	9	48	dep	modulation	1308:1317	arg1	demonstrated					1384:1395	demonstrated	1384:1395	demonstrated the strong synbiotic effect of BMOS and B. lactis on the infant gut microbiota	1384:1474	In this donor, preculturing B. lactis with BMOS strengthened further the early modulation of microbiota activity observed after 6 h. CONCLUSION This study demonstrated the strong synbiotic effect of BMOS and B. lactis on the infant gut microbiota, and suggests a strategy to improve its effectiveness in an otherwise low-Bifidobacterium microbiota.
32751149	5	49	theme	RESULTS	711:717	arg1	BMOS					719:722	RESULTS BMOS	711:722	RESULTS BMOS	711:722	RESULTS BMOS alone significantly induced acetate and lactate production (leading to pH decrease) and stimulated bifidobacterial growth in 10 donors.
32751149	4	50	theme	Short-term	470:479	arg1	fermentations					493:505	METHOD Short-term fecal batch fermentations	463:505	METHOD Short-term fecal batch fermentations (48 h)	463:512	METHOD Short-term fecal batch fermentations (48 h) were used to assess the microbial composition and activity modulated by BMOS alone, B. lactis grown on BMOS or dextrose alone, or their combinations on different three-month-old infant microbiota.
32751149	4	50	theme	Short-term	470:479	arg1	h					511:511	48 h	508:511	48 h	508:511	METHOD Short-term fecal batch fermentations (48 h) were used to assess the microbial composition and activity modulated by BMOS alone, B. lactis grown on BMOS or dextrose alone, or their combinations on different three-month-old infant microbiota.
32751149	8	51	theme	low	1191:1193	arg1	levels					1203:1208	low initial levels	1191:1208	low initial levels of bifidobacteria	1191:1226	This was also observed at the microbiota activity level, especially in a donor containing low initial levels of bifidobacteria.
32751149	8	52	theme	bifidobacteria	1213:1226	arg1	levels					1203:1208	low initial levels	1191:1208	low initial levels of bifidobacteria	1191:1226	This was also observed at the microbiota activity level, especially in a donor containing low initial levels of bifidobacteria.
32751149	6	53	from	study	879:883	arg1	donors					902:907	two different donors	888:907	two different donors	888:907	A further in-depth study on two different donors proved B. lactis ability to colonize the infant microbiota, regardless of the competitiveness of the environment.
32751149	8	54	theme	initial	1195:1201	arg1	levels					1203:1208	low initial levels	1191:1208	low initial levels of bifidobacteria	1191:1226	This was also observed at the microbiota activity level, especially in a donor containing low initial levels of bifidobacteria.
32751149	3	55	theme	novel	334:338	arg1	strategy					340:347	a novel strategy	332:347	a novel strategy consisting of preculturing B. lactis with BMOS to further enhance their potential synbiotic effects	332:447	In addition, a novel strategy consisting of preculturing B. lactis with BMOS to further enhance their potential synbiotic effects was assessed.
32751149	0	56	theme	I-3446	48:53	arg1	Effect					10:15	Synbiotic Effect	0:15	Synbiotic Effect of Bifidobacterium lactis CNCM I-3446 and Bovine Milk-Derived Oligosaccharides on Infant Gut Microbiota.	0:120	Synbiotic Effect of Bifidobacterium lactis CNCM I-3446 and Bovine Milk-Derived Oligosaccharides on Infant Gut Microbiota.
32751149	2	57	theme	Bovine	218:223	arg1	BMOS					256:259	BMOS	256:259	BMOS	256:259	lactis CNCM I-3446, Bovine Milk-derived OligoSaccharides (BMOS) and their combination on infant gut microbiota in vitro.
32751149	2	57	theme	Bovine	218:223	arg1	OligoSaccharides					238:253	Bovine Milk-derived OligoSaccharides	218:253	Bovine Milk-derived OligoSaccharides (BMOS)	218:260	lactis CNCM I-3446, Bovine Milk-derived OligoSaccharides (BMOS) and their combination on infant gut microbiota in vitro.
32751149	2	57	theme	Bovine	218:223	arg1	CNCM					205:208	CNCM	205:208	CNCM	205:208	lactis CNCM I-3446, Bovine Milk-derived OligoSaccharides (BMOS) and their combination on infant gut microbiota in vitro.
32751149	2	58	theme	Milk-derived	225:236	arg1	BMOS					256:259	BMOS	256:259	BMOS	256:259	lactis CNCM I-3446, Bovine Milk-derived OligoSaccharides (BMOS) and their combination on infant gut microbiota in vitro.
32751149	2	58	theme	Milk-derived	225:236	arg1	OligoSaccharides					238:253	Bovine Milk-derived OligoSaccharides	218:253	Bovine Milk-derived OligoSaccharides (BMOS)	218:260	lactis CNCM I-3446, Bovine Milk-derived OligoSaccharides (BMOS) and their combination on infant gut microbiota in vitro.
32751149	2	58	theme	Milk-derived	225:236	arg1	CNCM					205:208	CNCM	205:208	CNCM	205:208	lactis CNCM I-3446, Bovine Milk-derived OligoSaccharides (BMOS) and their combination on infant gut microbiota in vitro.
32751149	4	59	theme	infant	692:697	arg1	microbiota					699:708	different three-month-old infant microbiota	666:708	different three-month-old infant microbiota	666:708	METHOD Short-term fecal batch fermentations (48 h) were used to assess the microbial composition and activity modulated by BMOS alone, B. lactis grown on BMOS or dextrose alone, or their combinations on different three-month-old infant microbiota.
32751149	8	60	theme	microbiota	1131:1140	arg1	level					1151:1155	the microbiota activity level	1127:1155	the microbiota activity level	1127:1155	This was also observed at the microbiota activity level, especially in a donor containing low initial levels of bifidobacteria.
32751149	8	61	contain	containing	1180:1189	arg2	levels					1203:1208	low initial levels	1191:1208	low initial levels of bifidobacteria	1191:1226	This was also observed at the microbiota activity level, especially in a donor containing low initial levels of bifidobacteria.
32751149	8	61	contain	containing	1180:1189	arg1	donor					1174:1178	a donor	1172:1178	a donor containing low initial levels of bifidobacteria	1172:1226	This was also observed at the microbiota activity level, especially in a donor containing low initial levels of bifidobacteria.
32751149	5	62	from	growth	839:844	arg1	donors					852:857	10 donors	849:857	10 donors	849:857	RESULTS BMOS alone significantly induced acetate and lactate production (leading to pH decrease) and stimulated bifidobacterial growth in 10 donors.
32751149	9	63	theme	activity	1333:1340	arg1	modulation					1308:1317	the early modulation	1298:1317	the early modulation of microbiota activity observed after 6 h. CONCLUSION This study demonstrated the strong synbiotic effect of BMOS and B. lactis on the infant gut microbiota	1298:1474	In this donor, preculturing B. lactis with BMOS strengthened further the early modulation of microbiota activity observed after 6 h. CONCLUSION This study demonstrated the strong synbiotic effect of BMOS and B. lactis on the infant gut microbiota, and suggests a strategy to improve its effectiveness in an otherwise low-Bifidobacterium microbiota.
32751149	2	64	link	Milk-derived	225:236	arg1	BMOS					256:259	BMOS	256:259	BMOS	256:259	lactis CNCM I-3446, Bovine Milk-derived OligoSaccharides (BMOS) and their combination on infant gut microbiota in vitro.
32751149	2	64	link	Milk-derived	225:236	arg1	OligoSaccharides					238:253	Bovine Milk-derived OligoSaccharides	218:253	Bovine Milk-derived OligoSaccharides (BMOS)	218:260	lactis CNCM I-3446, Bovine Milk-derived OligoSaccharides (BMOS) and their combination on infant gut microbiota in vitro.
32751149	2	64	link	Milk-derived	225:236	arg1	CNCM					205:208	CNCM	205:208	CNCM	205:208	lactis CNCM I-3446, Bovine Milk-derived OligoSaccharides (BMOS) and their combination on infant gut microbiota in vitro.
32751149	0	65	theme	Milk-Derived	66:77	arg1	Oligosaccharides					79:94	Bovine Milk-Derived Oligosaccharides	59:94	Bovine Milk-Derived Oligosaccharides	59:94	Synbiotic Effect of Bifidobacterium lactis CNCM I-3446 and Bovine Milk-Derived Oligosaccharides on Infant Gut Microbiota.
32751149	9	66	theme	B.	1437:1438	arg1	effect					1418:1423	the strong synbiotic effect	1397:1423	the strong synbiotic effect of BMOS and B. lactis on the infant gut microbiota	1397:1474	In this donor, preculturing B. lactis with BMOS strengthened further the early modulation of microbiota activity observed after 6 h. CONCLUSION This study demonstrated the strong synbiotic effect of BMOS and B. lactis on the infant gut microbiota, and suggests a strategy to improve its effectiveness in an otherwise low-Bifidobacterium microbiota.
32751149	0	67	theme	Bovine	59:64	arg1	Oligosaccharides					79:94	Bovine Milk-Derived Oligosaccharides	59:94	Bovine Milk-Derived Oligosaccharides	59:94	Synbiotic Effect of Bifidobacterium lactis CNCM I-3446 and Bovine Milk-Derived Oligosaccharides on Infant Gut Microbiota.
32751149	6	68	theme	B.	916:917	arg1	ability					926:932	B. lactis ability	916:932	B. lactis ability	916:932	A further in-depth study on two different donors proved B. lactis ability to colonize the infant microbiota, regardless of the competitiveness of the environment.
32751149	9	69	theme	strong	1401:1406	arg1	effect					1418:1423	the strong synbiotic effect	1397:1423	the strong synbiotic effect of BMOS and B. lactis on the infant gut microbiota	1397:1474	In this donor, preculturing B. lactis with BMOS strengthened further the early modulation of microbiota activity observed after 6 h. CONCLUSION This study demonstrated the strong synbiotic effect of BMOS and B. lactis on the infant gut microbiota, and suggests a strategy to improve its effectiveness in an otherwise low-Bifidobacterium microbiota.
32751149	9	70	theme	h.	1359:1360	arg1	CONCLUSION					1362:1371	6 h. CONCLUSION	1357:1371	6 h. CONCLUSION	1357:1371	In this donor, preculturing B. lactis with BMOS strengthened further the early modulation of microbiota activity observed after 6 h. CONCLUSION This study demonstrated the strong synbiotic effect of BMOS and B. lactis on the infant gut microbiota, and suggests a strategy to improve its effectiveness in an otherwise low-Bifidobacterium microbiota.
32475525	2	0	theme	circulating	443:453	arg1	lipopolysaccharides					455:473	circulating lipopolysaccharides	443:473	circulating lipopolysaccharides	443:473	An imbalance or dysbiosis is directly associated with the development of several illnesses, due to the potential increase in intestinal permeability leading to a systemic inflammation triggered by higher levels of circulating lipopolysaccharides and changes in the immune response caused by an overgrowth of a specific genus or of pathogens.
32475525	7	1	theme	probiotics	1366:1375	arg1	roles					1342:1346	the potential roles	1328:1346	the potential roles of prebiotics and probiotics in health and diseases	1328:1398	In this chapter, we will discuss the potential roles of prebiotics and probiotics in health and diseases throughout an individual's lifetime and proposed mechanisms of action.
32475525	2	2	theme	pathogens	560:568	arg1	overgrowth					523:532	an overgrowth	520:532	an overgrowth of a specific genus or of pathogens	520:568	An imbalance or dysbiosis is directly associated with the development of several illnesses, due to the potential increase in intestinal permeability leading to a systemic inflammation triggered by higher levels of circulating lipopolysaccharides and changes in the immune response caused by an overgrowth of a specific genus or of pathogens.
32475525	2	3	theme	illnesses	310:318	arg1	development					287:297	the development	283:297	the development of several illnesses, due to the potential increase in intestinal permeability leading to a systemic inflammation triggered by higher levels of circulating lipopolysaccharides and changes in the immune response caused by an overgrowth of a specific genus or of pathogens	283:568	An imbalance or dysbiosis is directly associated with the development of several illnesses, due to the potential increase in intestinal permeability leading to a systemic inflammation triggered by higher levels of circulating lipopolysaccharides and changes in the immune response caused by an overgrowth of a specific genus or of pathogens.
32475525	6	4	theme	baseline	1257:1264	arg1	improvement					1242:1252	an improvement	1239:1252	an improvement of baseline and pathologic inflammation	1239:1292	Probiotic microorganism may increase the microbial diversity of GM and improve the integrity of the intestinal barrier, leading to an improvement of baseline and pathologic inflammation.
32475525	2	5	theme	several	302:308	arg1	illnesses					310:318	several illnesses	302:318	several illnesses	302:318	An imbalance or dysbiosis is directly associated with the development of several illnesses, due to the potential increase in intestinal permeability leading to a systemic inflammation triggered by higher levels of circulating lipopolysaccharides and changes in the immune response caused by an overgrowth of a specific genus or of pathogens.
32475525	2	6	theme	immune	494:499	arg1	response					501:508	the immune response	490:508	the immune response caused by an overgrowth of a specific genus or of pathogens	490:568	An imbalance or dysbiosis is directly associated with the development of several illnesses, due to the potential increase in intestinal permeability leading to a systemic inflammation triggered by higher levels of circulating lipopolysaccharides and changes in the immune response caused by an overgrowth of a specific genus or of pathogens.
32475525	5	7	theme	other	970:974	arg1	metabolites					976:986	other metabolites	970:986	other metabolites which may reduce the intestinal lumen pH, inhibit growth of pathogens, and enhance mineral and vitamin bioavailability	970:1105	Prebiotics, like inulin and polyphenols, are selectively utilized by GM, releasing short-chain fatty acids (SCFA) and other metabolites which may reduce the intestinal lumen pH, inhibit growth of pathogens, and enhance mineral and vitamin bioavailability.
32475525	3	8	from	symptoms	601:608	arg1	disorders					630:638	gastrointestinal disorders	613:638	gastrointestinal disorders	613:638	These mechanisms may increase symptoms in gastrointestinal disorders or reduce glucose tolerance in metabolic diseases.
32475525	2	9	theme	genus	548:552	arg1	overgrowth					523:532	an overgrowth	520:532	an overgrowth of a specific genus or of pathogens	520:568	An imbalance or dysbiosis is directly associated with the development of several illnesses, due to the potential increase in intestinal permeability leading to a systemic inflammation triggered by higher levels of circulating lipopolysaccharides and changes in the immune response caused by an overgrowth of a specific genus or of pathogens.
32475525	6	10	theme	barrier	1219:1225	arg1	integrity					1191:1199	the integrity	1187:1199	the integrity of the intestinal barrier	1187:1225	Probiotic microorganism may increase the microbial diversity of GM and improve the integrity of the intestinal barrier, leading to an improvement of baseline and pathologic inflammation.
32475525	4	11	contain	has	701:703	arg1	Diet					691:694	Diet	691:694	Diet	691:694	Diet also has a significant impact on GM, and functional foods, namely prebiotics and probiotics, are a novel approach to reestablish the indigenous microbiota.
32475525	4	11	contain	has	701:703	arg2	impact					719:724	a significant impact	705:724	a significant impact on GM	705:730	Diet also has a significant impact on GM, and functional foods, namely prebiotics and probiotics, are a novel approach to reestablish the indigenous microbiota.
32475525	4	12	theme	novel	795:799	arg1	approach					801:808	a novel approach	793:808	a novel approach to reestablish the indigenous microbiota	793:849	Diet also has a significant impact on GM, and functional foods, namely prebiotics and probiotics, are a novel approach to reestablish the indigenous microbiota.
32475525	4	12	theme	novel	795:799	arg1	foods					748:752	functional foods	737:752	functional foods	737:752	Diet also has a significant impact on GM, and functional foods, namely prebiotics and probiotics, are a novel approach to reestablish the indigenous microbiota.
32475525	4	13	theme	significant	707:717	arg1	impact					719:724	a significant impact	705:724	a significant impact on GM	705:730	Diet also has a significant impact on GM, and functional foods, namely prebiotics and probiotics, are a novel approach to reestablish the indigenous microbiota.
32475525	4	14	theme	functional	737:746	arg1	approach					801:808	a novel approach	793:808	a novel approach to reestablish the indigenous microbiota	793:849	Diet also has a significant impact on GM, and functional foods, namely prebiotics and probiotics, are a novel approach to reestablish the indigenous microbiota.
32475525	4	14	theme	functional	737:746	arg1	probiotics					777:786	probiotics	777:786	probiotics	777:786	Diet also has a significant impact on GM, and functional foods, namely prebiotics and probiotics, are a novel approach to reestablish the indigenous microbiota.
32475525	4	14	theme	functional	737:746	arg1	foods					748:752	functional foods	737:752	functional foods	737:752	Diet also has a significant impact on GM, and functional foods, namely prebiotics and probiotics, are a novel approach to reestablish the indigenous microbiota.
32475525	4	14	theme	functional	737:746	arg1	prebiotics					762:771	prebiotics	762:771	prebiotics	762:771	Diet also has a significant impact on GM, and functional foods, namely prebiotics and probiotics, are a novel approach to reestablish the indigenous microbiota.
32475525	1	15	theme	intrinsic	149:157	arg1	factors					220:226	intrinsic (genetics, age) and extrinsic (environment, diet, lifestyle) factors	149:226	intrinsic (genetics, age) and extrinsic (environment, diet, lifestyle) factors	149:226	The gut microbiota (GM) composition varies among individuals and is influenced by intrinsic (genetics, age) and extrinsic (environment, diet, lifestyle) factors.
32475525	2	16	from	levels	433:438	arg1	response					501:508	the immune response	490:508	the immune response caused by an overgrowth of a specific genus or of pathogens	490:568	An imbalance or dysbiosis is directly associated with the development of several illnesses, due to the potential increase in intestinal permeability leading to a systemic inflammation triggered by higher levels of circulating lipopolysaccharides and changes in the immune response caused by an overgrowth of a specific genus or of pathogens.
32475525	1	17	theme	gut	71:73	arg1	composition					91:101	The gut microbiota (GM) composition	67:101	The gut microbiota (GM) composition	67:101	The gut microbiota (GM) composition varies among individuals and is influenced by intrinsic (genetics, age) and extrinsic (environment, diet, lifestyle) factors.
32475525	2	18	theme	potential	332:340	arg1	increase					342:349	the potential increase	328:349	the potential increase in intestinal permeability leading to a systemic inflammation triggered by higher levels of circulating lipopolysaccharides and changes in the immune response caused by an overgrowth of a specific genus or of pathogens	328:568	An imbalance or dysbiosis is directly associated with the development of several illnesses, due to the potential increase in intestinal permeability leading to a systemic inflammation triggered by higher levels of circulating lipopolysaccharides and changes in the immune response caused by an overgrowth of a specific genus or of pathogens.
32475525	5	19	theme	pathogens	1048:1056	arg1	growth					1038:1043	growth	1038:1043	growth of pathogens	1038:1056	Prebiotics, like inulin and polyphenols, are selectively utilized by GM, releasing short-chain fatty acids (SCFA) and other metabolites which may reduce the intestinal lumen pH, inhibit growth of pathogens, and enhance mineral and vitamin bioavailability.
32475525	4	20	from	impact	719:724	arg1	GM					729:730	GM	729:730	GM	729:730	Diet also has a significant impact on GM, and functional foods, namely prebiotics and probiotics, are a novel approach to reestablish the indigenous microbiota.
32475525	1	21	theme	microbiota	75:84	arg1	composition					91:101	The gut microbiota (GM) composition	67:101	The gut microbiota (GM) composition	67:101	The gut microbiota (GM) composition varies among individuals and is influenced by intrinsic (genetics, age) and extrinsic (environment, diet, lifestyle) factors.
32475525	7	22	from	roles	1342:1346	arg1	diseases					1391:1398	diseases	1391:1398	diseases	1391:1398	In this chapter, we will discuss the potential roles of prebiotics and probiotics in health and diseases throughout an individual's lifetime and proposed mechanisms of action.
32475525	7	22	from	roles	1342:1346	arg1	health					1380:1385	health	1380:1385	health	1380:1385	In this chapter, we will discuss the potential roles of prebiotics and probiotics in health and diseases throughout an individual's lifetime and proposed mechanisms of action.
32475525	2	23	theme	systemic	391:398	arg1	inflammation					400:411	a systemic inflammation	389:411	a systemic inflammation triggered by higher levels of circulating lipopolysaccharides and changes in the immune response caused by an overgrowth of a specific genus or of pathogens	389:568	An imbalance or dysbiosis is directly associated with the development of several illnesses, due to the potential increase in intestinal permeability leading to a systemic inflammation triggered by higher levels of circulating lipopolysaccharides and changes in the immune response caused by an overgrowth of a specific genus or of pathogens.
32475525	6	24	theme	intestinal	1208:1217	arg1	barrier					1219:1225	the intestinal barrier	1204:1225	the intestinal barrier	1204:1225	Probiotic microorganism may increase the microbial diversity of GM and improve the integrity of the intestinal barrier, leading to an improvement of baseline and pathologic inflammation.
32475525	5	25	theme	lumen	1020:1024	arg1	pH					1026:1027	the intestinal lumen pH	1005:1027	the intestinal lumen pH	1005:1027	Prebiotics, like inulin and polyphenols, are selectively utilized by GM, releasing short-chain fatty acids (SCFA) and other metabolites which may reduce the intestinal lumen pH, inhibit growth of pathogens, and enhance mineral and vitamin bioavailability.
32475525	7	26	theme	prebiotics	1351:1360	arg1	roles					1342:1346	the potential roles	1328:1346	the potential roles of prebiotics and probiotics in health and diseases	1328:1398	In this chapter, we will discuss the potential roles of prebiotics and probiotics in health and diseases throughout an individual's lifetime and proposed mechanisms of action.
32475525	6	27	theme	GM	1172:1173	arg1	diversity					1159:1167	the microbial diversity	1145:1167	the microbial diversity of GM	1145:1173	Probiotic microorganism may increase the microbial diversity of GM and improve the integrity of the intestinal barrier, leading to an improvement of baseline and pathologic inflammation.
32475525	1	28	theme	GM	87:88	arg1	composition					91:101	The gut microbiota (GM) composition	67:101	The gut microbiota (GM) composition	67:101	The gut microbiota (GM) composition varies among individuals and is influenced by intrinsic (genetics, age) and extrinsic (environment, diet, lifestyle) factors.
32475525	0	29	theme	probiotics	16:25	arg1	Interactions					0:11	Interactions	0:11	Interactions of probiotics and prebiotics with the gut microbiota	0:64	Interactions of probiotics and prebiotics with the gut microbiota.
32475525	6	30	theme	inflammation	1281:1292	arg1	improvement					1242:1252	an improvement	1239:1252	an improvement of baseline and pathologic inflammation	1239:1292	Probiotic microorganism may increase the microbial diversity of GM and improve the integrity of the intestinal barrier, leading to an improvement of baseline and pathologic inflammation.
32475525	5	31	theme	intestinal	1009:1018	arg1	lumen					1020:1024	the intestinal lumen	1005:1024	the intestinal lumen pH	1005:1027	Prebiotics, like inulin and polyphenols, are selectively utilized by GM, releasing short-chain fatty acids (SCFA) and other metabolites which may reduce the intestinal lumen pH, inhibit growth of pathogens, and enhance mineral and vitamin bioavailability.
32475525	3	32	from	tolerance	658:666	arg1	diseases					681:688	metabolic diseases	671:688	metabolic diseases	671:688	These mechanisms may increase symptoms in gastrointestinal disorders or reduce glucose tolerance in metabolic diseases.
32475525	6	33	theme	pathologic	1270:1279	arg1	inflammation					1281:1292	pathologic inflammation	1270:1292	pathologic inflammation	1270:1292	Probiotic microorganism may increase the microbial diversity of GM and improve the integrity of the intestinal barrier, leading to an improvement of baseline and pathologic inflammation.
32475525	3	34	theme	gastrointestinal	613:628	arg1	disorders					630:638	gastrointestinal disorders	613:638	gastrointestinal disorders	613:638	These mechanisms may increase symptoms in gastrointestinal disorders or reduce glucose tolerance in metabolic diseases.
32475525	7	35	theme	action	1463:1468	arg1	lifetime					1427:1434	an individual's lifetime	1411:1434	an individual's lifetime	1411:1434	In this chapter, we will discuss the potential roles of prebiotics and probiotics in health and diseases throughout an individual's lifetime and proposed mechanisms of action.
32475525	7	35	theme	action	1463:1468	arg1	mechanisms					1449:1458	proposed mechanisms	1440:1458	proposed mechanisms of action	1440:1468	In this chapter, we will discuss the potential roles of prebiotics and probiotics in health and diseases throughout an individual's lifetime and proposed mechanisms of action.
32475525	2	36	theme	intestinal	354:363	arg1	permeability					365:376	intestinal permeability	354:376	intestinal permeability leading to a systemic inflammation triggered by higher levels of circulating lipopolysaccharides and changes in the immune response caused by an overgrowth of a specific genus or of pathogens	354:568	An imbalance or dysbiosis is directly associated with the development of several illnesses, due to the potential increase in intestinal permeability leading to a systemic inflammation triggered by higher levels of circulating lipopolysaccharides and changes in the immune response caused by an overgrowth of a specific genus or of pathogens.
32475525	2	37	theme	higher	426:431	arg1	levels					433:438	higher levels	426:438	higher levels of circulating lipopolysaccharides	426:473	An imbalance or dysbiosis is directly associated with the development of several illnesses, due to the potential increase in intestinal permeability leading to a systemic inflammation triggered by higher levels of circulating lipopolysaccharides and changes in the immune response caused by an overgrowth of a specific genus or of pathogens.
32475525	0	38	theme	prebiotics	31:40	arg1	Interactions					0:11	Interactions	0:11	Interactions of probiotics and prebiotics with the gut microbiota	0:64	Interactions of probiotics and prebiotics with the gut microbiota.
32475525	1	39	theme	extrinsic	179:187	arg1	factors					220:226	intrinsic (genetics, age) and extrinsic (environment, diet, lifestyle) factors	149:226	intrinsic (genetics, age) and extrinsic (environment, diet, lifestyle) factors	149:226	The gut microbiota (GM) composition varies among individuals and is influenced by intrinsic (genetics, age) and extrinsic (environment, diet, lifestyle) factors.
32475525	0	40	theme	gut	51:53	arg1	microbiota					55:64	the gut microbiota	47:64	the gut microbiota	47:64	Interactions of probiotics and prebiotics with the gut microbiota.
32475525	5	41	theme	vitamin	1083:1089	arg1	bioavailability					1091:1105	vitamin bioavailability	1083:1105	vitamin bioavailability	1083:1105	Prebiotics, like inulin and polyphenols, are selectively utilized by GM, releasing short-chain fatty acids (SCFA) and other metabolites which may reduce the intestinal lumen pH, inhibit growth of pathogens, and enhance mineral and vitamin bioavailability.
32475525	2	42	theme	specific	539:546	arg1	genus					548:552	a specific genus	537:552	a specific genus	537:552	An imbalance or dysbiosis is directly associated with the development of several illnesses, due to the potential increase in intestinal permeability leading to a systemic inflammation triggered by higher levels of circulating lipopolysaccharides and changes in the immune response caused by an overgrowth of a specific genus or of pathogens.
32475525	1	43	dep	intrinsic	149:157	arg1	genetics					160:167	genetics	160:167	genetics	160:167	The gut microbiota (GM) composition varies among individuals and is influenced by intrinsic (genetics, age) and extrinsic (environment, diet, lifestyle) factors.
32475525	1	43	dep	intrinsic	149:157	arg1	age					170:172	age	170:172	age	170:172	The gut microbiota (GM) composition varies among individuals and is influenced by intrinsic (genetics, age) and extrinsic (environment, diet, lifestyle) factors.
32475525	3	44	theme	glucose	650:656	arg1	tolerance					658:666	glucose tolerance	650:666	glucose tolerance in metabolic diseases	650:688	These mechanisms may increase symptoms in gastrointestinal disorders or reduce glucose tolerance in metabolic diseases.
32475525	2	45	from	increase	342:349	arg1	permeability					365:376	intestinal permeability	354:376	intestinal permeability leading to a systemic inflammation triggered by higher levels of circulating lipopolysaccharides and changes in the immune response caused by an overgrowth of a specific genus or of pathogens	354:568	An imbalance or dysbiosis is directly associated with the development of several illnesses, due to the potential increase in intestinal permeability leading to a systemic inflammation triggered by higher levels of circulating lipopolysaccharides and changes in the immune response caused by an overgrowth of a specific genus or of pathogens.
32475525	2	46	from	changes	479:485	arg1	response					501:508	the immune response	490:508	the immune response caused by an overgrowth of a specific genus or of pathogens	490:568	An imbalance or dysbiosis is directly associated with the development of several illnesses, due to the potential increase in intestinal permeability leading to a systemic inflammation triggered by higher levels of circulating lipopolysaccharides and changes in the immune response caused by an overgrowth of a specific genus or of pathogens.
32475525	5	47	theme	fatty	947:951	arg1	SCFA					960:963	SCFA	960:963	SCFA	960:963	Prebiotics, like inulin and polyphenols, are selectively utilized by GM, releasing short-chain fatty acids (SCFA) and other metabolites which may reduce the intestinal lumen pH, inhibit growth of pathogens, and enhance mineral and vitamin bioavailability.
32475525	5	47	theme	fatty	947:951	arg1	acids					953:957	short-chain fatty acids	935:957	short-chain fatty acids (SCFA)	935:964	Prebiotics, like inulin and polyphenols, are selectively utilized by GM, releasing short-chain fatty acids (SCFA) and other metabolites which may reduce the intestinal lumen pH, inhibit growth of pathogens, and enhance mineral and vitamin bioavailability.
32475525	0	48	with	Interactions	0:11	arg1	microbiota					55:64	the gut microbiota	47:64	the gut microbiota	47:64	Interactions of probiotics and prebiotics with the gut microbiota.
32475525	7	49	theme	potential	1332:1340	arg1	roles					1342:1346	the potential roles	1328:1346	the potential roles of prebiotics and probiotics in health and diseases	1328:1398	In this chapter, we will discuss the potential roles of prebiotics and probiotics in health and diseases throughout an individual's lifetime and proposed mechanisms of action.
32475525	5	50	theme	short-chain	935:945	arg1	SCFA					960:963	SCFA	960:963	SCFA	960:963	Prebiotics, like inulin and polyphenols, are selectively utilized by GM, releasing short-chain fatty acids (SCFA) and other metabolites which may reduce the intestinal lumen pH, inhibit growth of pathogens, and enhance mineral and vitamin bioavailability.
32475525	5	50	theme	short-chain	935:945	arg1	acids					953:957	short-chain fatty acids	935:957	short-chain fatty acids (SCFA)	935:964	Prebiotics, like inulin and polyphenols, are selectively utilized by GM, releasing short-chain fatty acids (SCFA) and other metabolites which may reduce the intestinal lumen pH, inhibit growth of pathogens, and enhance mineral and vitamin bioavailability.
32475525	7	51	theme	proposed	1440:1447	arg1	mechanisms					1449:1458	proposed mechanisms	1440:1458	proposed mechanisms of action	1440:1468	In this chapter, we will discuss the potential roles of prebiotics and probiotics in health and diseases throughout an individual's lifetime and proposed mechanisms of action.
32475525	1	52	dep	extrinsic	179:187	arg1	lifestyle					209:217	lifestyle	209:217	lifestyle	209:217	The gut microbiota (GM) composition varies among individuals and is influenced by intrinsic (genetics, age) and extrinsic (environment, diet, lifestyle) factors.
32475525	1	52	dep	extrinsic	179:187	arg1	diet					203:206	diet	203:206	diet	203:206	The gut microbiota (GM) composition varies among individuals and is influenced by intrinsic (genetics, age) and extrinsic (environment, diet, lifestyle) factors.
32475525	1	52	dep	extrinsic	179:187	arg1	environment					190:200	environment	190:200	environment	190:200	The gut microbiota (GM) composition varies among individuals and is influenced by intrinsic (genetics, age) and extrinsic (environment, diet, lifestyle) factors.
32475525	4	53	theme	indigenous	829:838	arg1	microbiota					840:849	the indigenous microbiota	825:849	the indigenous microbiota	825:849	Diet also has a significant impact on GM, and functional foods, namely prebiotics and probiotics, are a novel approach to reestablish the indigenous microbiota.
32475525	6	54	theme	microbial	1149:1157	arg1	diversity					1159:1167	the microbial diversity	1145:1167	the microbial diversity of GM	1145:1173	Probiotic microorganism may increase the microbial diversity of GM and improve the integrity of the intestinal barrier, leading to an improvement of baseline and pathologic inflammation.
32475525	6	55	theme	Probiotic	1108:1116	arg1	microorganism					1118:1130	Probiotic microorganism	1108:1130	Probiotic microorganism	1108:1130	Probiotic microorganism may increase the microbial diversity of GM and improve the integrity of the intestinal barrier, leading to an improvement of baseline and pathologic inflammation.
32475525	2	56	theme	due	321:323	arg1	illnesses					310:318	several illnesses	302:318	several illnesses	302:318	An imbalance or dysbiosis is directly associated with the development of several illnesses, due to the potential increase in intestinal permeability leading to a systemic inflammation triggered by higher levels of circulating lipopolysaccharides and changes in the immune response caused by an overgrowth of a specific genus or of pathogens.
32475525	5	57	used	utilized	909:916	arg2	Prebiotics					852:861	Prebiotics	852:861	Prebiotics	852:861	Prebiotics, like inulin and polyphenols, are selectively utilized by GM, releasing short-chain fatty acids (SCFA) and other metabolites which may reduce the intestinal lumen pH, inhibit growth of pathogens, and enhance mineral and vitamin bioavailability.
32475525	2	58	theme	lipopolysaccharides	455:473	arg1	changes					479:485	changes	479:485	changes in the immune response caused by an overgrowth of a specific genus or of pathogens	479:568	An imbalance or dysbiosis is directly associated with the development of several illnesses, due to the potential increase in intestinal permeability leading to a systemic inflammation triggered by higher levels of circulating lipopolysaccharides and changes in the immune response caused by an overgrowth of a specific genus or of pathogens.
32475525	2	58	theme	lipopolysaccharides	455:473	arg1	levels					433:438	higher levels	426:438	higher levels of circulating lipopolysaccharides	426:473	An imbalance or dysbiosis is directly associated with the development of several illnesses, due to the potential increase in intestinal permeability leading to a systemic inflammation triggered by higher levels of circulating lipopolysaccharides and changes in the immune response caused by an overgrowth of a specific genus or of pathogens.
32475525	3	59	theme	metabolic	671:679	arg1	diseases					681:688	metabolic diseases	671:688	metabolic diseases	671:688	These mechanisms may increase symptoms in gastrointestinal disorders or reduce glucose tolerance in metabolic diseases.
31990281	4	0	theme	=	1004:1004	arg1	S/N					1000:1002	S/N = 3	1000:1006	S/N = 3	1000:1006	In addition, a great linear range was obtained with a concentration range of 0.08-100 μM and a low detection limit of 0.037 μM (S/N = 3).
31990281	4	0	theme	=	1004:1004	arg1	limit					981:985	a low detection limit	965:985	a low detection limit of 0.037 μM (S/N = 3)	965:1007	In addition, a great linear range was obtained with a concentration range of 0.08-100 μM and a low detection limit of 0.037 μM (S/N = 3).
31990281	3	1	theme	cyclic	733:738	arg1	voltammetry					740:750	cyclic voltammetry	733:750	cyclic voltammetry	733:750	The sensor was then evaluated for its ability to detect ofloxacin using cyclic voltammetry, and the results showed that the PtNPs/KB/CD-MOF had high sensitivity, satisfactory stability and good reproducibility.
31990281	0	2	theme	ofloxacin	170:178	arg1	detection					157:165	the electrochemical detection	137:165	the electrochemical detection of ofloxacin	137:178	Facile synthesis of a cyclodextrin-metal organic framework decorated with Ketjen Black and platinum nanoparticles and its application in the electrochemical detection of ofloxacin.
31990281	2	3	theme	electron	492:499	arg1	microscopy					501:510	transmission electron microscopy	479:510	transmission electron microscopy	479:510	The structural morphology and composition of this PtNPs/KB/CD-MOFs nanocomposite was characterized by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, elemental mapping, and Raman spectroscopy.
31990281	4	4	theme	linear	893:898	arg1	range					900:904	a great linear range	885:904	a great linear range	885:904	In addition, a great linear range was obtained with a concentration range of 0.08-100 μM and a low detection limit of 0.037 μM (S/N = 3).
31990281	1	5	theme	cyclodextrin-metal	254:271	arg1	CD-MOFs					293:299	CD-MOFs	293:299	CD-MOFs	293:299	A facile and novel method was developed to improve the conductivity of a cyclodextrin-metal organic frameworks (CD-MOFs) by modifying it with Ketjen Black (KB) and platinum nanoparticles (PtNPs).
31990281	1	5	theme	cyclodextrin-metal	254:271	arg1	frameworks					281:290	a cyclodextrin-metal organic frameworks	252:290	a cyclodextrin-metal organic frameworks (CD-MOFs)	252:300	A facile and novel method was developed to improve the conductivity of a cyclodextrin-metal organic frameworks (CD-MOFs) by modifying it with Ketjen Black (KB) and platinum nanoparticles (PtNPs).
31990281	4	6	theme	low	967:969	arg1	S/N					1000:1002	S/N = 3	1000:1006	S/N = 3	1000:1006	In addition, a great linear range was obtained with a concentration range of 0.08-100 μM and a low detection limit of 0.037 μM (S/N = 3).
31990281	4	6	theme	low	967:969	arg1	limit					981:985	a low detection limit	965:985	a low detection limit of 0.037 μM (S/N = 3)	965:1007	In addition, a great linear range was obtained with a concentration range of 0.08-100 μM and a low detection limit of 0.037 μM (S/N = 3).
31990281	3	7	contain	had	801:803	arg2	sensitivity					810:820	high sensitivity	805:820	high sensitivity	805:820	The sensor was then evaluated for its ability to detect ofloxacin using cyclic voltammetry, and the results showed that the PtNPs/KB/CD-MOF had high sensitivity, satisfactory stability and good reproducibility.
31990281	3	7	contain	had	801:803	arg2	reproducibility					855:869	good reproducibility	850:869	good reproducibility	850:869	The sensor was then evaluated for its ability to detect ofloxacin using cyclic voltammetry, and the results showed that the PtNPs/KB/CD-MOF had high sensitivity, satisfactory stability and good reproducibility.
31990281	3	7	contain	had	801:803	arg1	PtNPs/KB/CD-MOF					785:799	the PtNPs/KB/CD-MOF	781:799	the PtNPs/KB/CD-MOF	781:799	The sensor was then evaluated for its ability to detect ofloxacin using cyclic voltammetry, and the results showed that the PtNPs/KB/CD-MOF had high sensitivity, satisfactory stability and good reproducibility.
31990281	3	7	contain	had	801:803	arg2	stability					836:844	satisfactory stability	823:844	satisfactory stability	823:844	The sensor was then evaluated for its ability to detect ofloxacin using cyclic voltammetry, and the results showed that the PtNPs/KB/CD-MOF had high sensitivity, satisfactory stability and good reproducibility.
31990281	1	8	theme	organic	273:279	arg1	CD-MOFs					293:299	CD-MOFs	293:299	CD-MOFs	293:299	A facile and novel method was developed to improve the conductivity of a cyclodextrin-metal organic frameworks (CD-MOFs) by modifying it with Ketjen Black (KB) and platinum nanoparticles (PtNPs).
31990281	1	8	theme	organic	273:279	arg1	frameworks					281:290	a cyclodextrin-metal organic frameworks	252:290	a cyclodextrin-metal organic frameworks (CD-MOFs)	252:300	A facile and novel method was developed to improve the conductivity of a cyclodextrin-metal organic frameworks (CD-MOFs) by modifying it with Ketjen Black (KB) and platinum nanoparticles (PtNPs).
31990281	0	9	theme	platinum	91:98	arg1	nanoparticles					100:112	Ketjen Black and platinum nanoparticles	74:112	nanoparticles	100:112	Facile synthesis of a cyclodextrin-metal organic framework decorated with Ketjen Black and platinum nanoparticles and its application in the electrochemical detection of ofloxacin.
31990281	2	10	theme	elemental	618:626	arg1	mapping					628:634	elemental mapping	618:634	elemental mapping	618:634	The structural morphology and composition of this PtNPs/KB/CD-MOFs nanocomposite was characterized by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, elemental mapping, and Raman spectroscopy.
31990281	1	11	theme	frameworks	281:290	arg1	conductivity					236:247	the conductivity	232:247	the conductivity of a cyclodextrin-metal organic frameworks (CD-MOFs)	232:300	A facile and novel method was developed to improve the conductivity of a cyclodextrin-metal organic frameworks (CD-MOFs) by modifying it with Ketjen Black (KB) and platinum nanoparticles (PtNPs).
31990281	1	12	theme	platinum	345:352	arg1	nanoparticles					354:366	Ketjen Black (KB) and platinum nanoparticles	323:366	nanoparticles	354:366	A facile and novel method was developed to improve the conductivity of a cyclodextrin-metal organic frameworks (CD-MOFs) by modifying it with Ketjen Black (KB) and platinum nanoparticles (PtNPs).
31990281	2	13	theme	electron	522:529	arg1	microscopy					531:540	scanning electron microscopy	513:540	scanning electron microscopy	513:540	The structural morphology and composition of this PtNPs/KB/CD-MOFs nanocomposite was characterized by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, elemental mapping, and Raman spectroscopy.
31990281	0	14	theme	organic	41:47	arg1	framework					49:57	a cyclodextrin-metal organic framework	20:57	a cyclodextrin-metal organic framework	20:57	Facile synthesis of a cyclodextrin-metal organic framework decorated with Ketjen Black and platinum nanoparticles and its application in the electrochemical detection of ofloxacin.
31990281	2	15	theme	PtNPs/KB/CD-MOFs	427:442	arg1	nanocomposite					444:456	this PtNPs/KB/CD-MOFs nanocomposite	422:456	this PtNPs/KB/CD-MOFs nanocomposite	422:456	The structural morphology and composition of this PtNPs/KB/CD-MOFs nanocomposite was characterized by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, elemental mapping, and Raman spectroscopy.
31990281	1	16	theme	facile	183:188	arg1	method					200:205	A facile and novel method	181:205	A facile and novel method	181:205	A facile and novel method was developed to improve the conductivity of a cyclodextrin-metal organic frameworks (CD-MOFs) by modifying it with Ketjen Black (KB) and platinum nanoparticles (PtNPs).
31990281	2	17	theme	nanocomposite	444:456	arg1	morphology					392:401	structural morphology	381:401	structural morphology	381:401	The structural morphology and composition of this PtNPs/KB/CD-MOFs nanocomposite was characterized by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, elemental mapping, and Raman spectroscopy.
31990281	2	17	theme	nanocomposite	444:456	arg1	composition					407:417	composition	407:417	composition	407:417	The structural morphology and composition of this PtNPs/KB/CD-MOFs nanocomposite was characterized by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, elemental mapping, and Raman spectroscopy.
31990281	4	18	theme	concentration	926:938	arg1	range					940:944	a concentration range	924:944	a concentration range of 0.08-100 μM	924:959	In addition, a great linear range was obtained with a concentration range of 0.08-100 μM and a low detection limit of 0.037 μM (S/N = 3).
31990281	4	19	theme	great	887:891	arg1	range					900:904	a great linear range	885:904	a great linear range	885:904	In addition, a great linear range was obtained with a concentration range of 0.08-100 μM and a low detection limit of 0.037 μM (S/N = 3).
31990281	0	20	theme	Facile	0:5	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis of a cyclodextrin-metal organic framework	0:57	Facile synthesis of a cyclodextrin-metal organic framework decorated with Ketjen Black and platinum nanoparticles and its application in the electrochemical detection of ofloxacin.
31990281	2	21	theme	Raman	641:645	arg1	spectroscopy					647:658	Raman spectroscopy	641:658	Raman spectroscopy	641:658	The structural morphology and composition of this PtNPs/KB/CD-MOFs nanocomposite was characterized by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, elemental mapping, and Raman spectroscopy.
31990281	1	22	theme	novel	194:198	arg1	method					200:205	A facile and novel method	181:205	A facile and novel method	181:205	A facile and novel method was developed to improve the conductivity of a cyclodextrin-metal organic frameworks (CD-MOFs) by modifying it with Ketjen Black (KB) and platinum nanoparticles (PtNPs).
31990281	2	23	theme	transmission	479:490	arg1	microscopy					501:510	transmission electron microscopy	479:510	transmission electron microscopy	479:510	The structural morphology and composition of this PtNPs/KB/CD-MOFs nanocomposite was characterized by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, elemental mapping, and Raman spectroscopy.
31990281	0	24	theme	electrochemical	141:155	arg1	detection					157:165	the electrochemical detection	137:165	the electrochemical detection of ofloxacin	137:178	Facile synthesis of a cyclodextrin-metal organic framework decorated with Ketjen Black and platinum nanoparticles and its application in the electrochemical detection of ofloxacin.
31990281	2	25	dep	morphology	392:401	arg1	The					377:379	The	377:379	The	377:379	The structural morphology and composition of this PtNPs/KB/CD-MOFs nanocomposite was characterized by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, elemental mapping, and Raman spectroscopy.
31990281	0	26	theme	cyclodextrin-metal	22:39	arg1	framework					49:57	a cyclodextrin-metal organic framework	20:57	a cyclodextrin-metal organic framework	20:57	Facile synthesis of a cyclodextrin-metal organic framework decorated with Ketjen Black and platinum nanoparticles and its application in the electrochemical detection of ofloxacin.
31990281	3	27	theme	high	805:808	arg1	sensitivity					810:820	high sensitivity	805:820	high sensitivity	805:820	The sensor was then evaluated for its ability to detect ofloxacin using cyclic voltammetry, and the results showed that the PtNPs/KB/CD-MOF had high sensitivity, satisfactory stability and good reproducibility.
31990281	4	28	theme	μM	958:959	arg1	limit					981:985	a low detection limit	965:985	a low detection limit of 0.037 μM (S/N = 3)	965:1007	In addition, a great linear range was obtained with a concentration range of 0.08-100 μM and a low detection limit of 0.037 μM (S/N = 3).
31990281	4	28	theme	μM	958:959	arg1	S/N					1000:1002	S/N = 3	1000:1006	S/N = 3	1000:1006	In addition, a great linear range was obtained with a concentration range of 0.08-100 μM and a low detection limit of 0.037 μM (S/N = 3).
31990281	4	28	theme	μM	958:959	arg1	range					940:944	a concentration range	924:944	a concentration range of 0.08-100 μM	924:959	In addition, a great linear range was obtained with a concentration range of 0.08-100 μM and a low detection limit of 0.037 μM (S/N = 3).
31990281	2	29	theme	structural	381:390	arg1	morphology					392:401	structural morphology	381:401	structural morphology	381:401	The structural morphology and composition of this PtNPs/KB/CD-MOFs nanocomposite was characterized by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, elemental mapping, and Raman spectroscopy.
31990281	2	30	dep	Fourier	543:549	arg1	transform					551:559	transform	551:559	transform infrared spectroscopy	551:581	The structural morphology and composition of this PtNPs/KB/CD-MOFs nanocomposite was characterized by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, elemental mapping, and Raman spectroscopy.
31990281	5	31	used	used	1079:1082	arg2	sensor					1050:1055	the prepared electrochemical sensor	1021:1055	the prepared electrochemical sensor	1021:1055	Therefore, the prepared electrochemical sensor could be successfully used to detect ofloxacin in serum.
31990281	0	32	theme	framework	49:57	arg1	synthesis					7:15	Facile synthesis	0:15	Facile synthesis of a cyclodextrin-metal organic framework	0:57	Facile synthesis of a cyclodextrin-metal organic framework decorated with Ketjen Black and platinum nanoparticles and its application in the electrochemical detection of ofloxacin.
31990281	5	33	theme	prepared	1025:1032	arg1	sensor					1050:1055	the prepared electrochemical sensor	1021:1055	the prepared electrochemical sensor	1021:1055	Therefore, the prepared electrochemical sensor could be successfully used to detect ofloxacin in serum.
31990281	1	34	theme	Ketjen	323:328	arg1	KB					337:338	Ketjen Black (KB) and platinum nanoparticles	323:366	KB	337:338	A facile and novel method was developed to improve the conductivity of a cyclodextrin-metal organic frameworks (CD-MOFs) by modifying it with Ketjen Black (KB) and platinum nanoparticles (PtNPs).
31990281	1	34	theme	Ketjen	323:328	arg1	PtNPs					369:373	PtNPs	369:373	PtNPs	369:373	A facile and novel method was developed to improve the conductivity of a cyclodextrin-metal organic frameworks (CD-MOFs) by modifying it with Ketjen Black (KB) and platinum nanoparticles (PtNPs).
31990281	2	35	theme	photoelectron	590:602	arg1	spectroscopy					604:615	X-ray photoelectron spectroscopy	584:615	X-ray photoelectron spectroscopy	584:615	The structural morphology and composition of this PtNPs/KB/CD-MOFs nanocomposite was characterized by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, elemental mapping, and Raman spectroscopy.
31990281	5	36	theme	electrochemical	1034:1048	arg1	sensor					1050:1055	the prepared electrochemical sensor	1021:1055	the prepared electrochemical sensor	1021:1055	Therefore, the prepared electrochemical sensor could be successfully used to detect ofloxacin in serum.
31990281	4	37	theme	μM	996:997	arg1	limit					981:985	a low detection limit	965:985	a low detection limit of 0.037 μM (S/N = 3)	965:1007	In addition, a great linear range was obtained with a concentration range of 0.08-100 μM and a low detection limit of 0.037 μM (S/N = 3).
31990281	4	37	theme	μM	996:997	arg1	S/N					1000:1002	S/N = 3	1000:1006	S/N = 3	1000:1006	In addition, a great linear range was obtained with a concentration range of 0.08-100 μM and a low detection limit of 0.037 μM (S/N = 3).
31990281	4	37	theme	μM	996:997	arg1	range					940:944	a concentration range	924:944	a concentration range of 0.08-100 μM	924:959	In addition, a great linear range was obtained with a concentration range of 0.08-100 μM and a low detection limit of 0.037 μM (S/N = 3).
31990281	2	38	theme	X-ray	584:588	arg1	spectroscopy					604:615	X-ray photoelectron spectroscopy	584:615	X-ray photoelectron spectroscopy	584:615	The structural morphology and composition of this PtNPs/KB/CD-MOFs nanocomposite was characterized by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, elemental mapping, and Raman spectroscopy.
31990281	2	39	theme	scanning	513:520	arg1	microscopy					531:540	scanning electron microscopy	513:540	scanning electron microscopy	513:540	The structural morphology and composition of this PtNPs/KB/CD-MOFs nanocomposite was characterized by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, elemental mapping, and Raman spectroscopy.
31990281	2	40	dep	transform	551:559	arg1	infrared					561:568	infrared	561:568	transform infrared spectroscopy	551:581	The structural morphology and composition of this PtNPs/KB/CD-MOFs nanocomposite was characterized by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, elemental mapping, and Raman spectroscopy.
31990281	3	41	theme	good	850:853	arg1	reproducibility					855:869	good reproducibility	850:869	good reproducibility	850:869	The sensor was then evaluated for its ability to detect ofloxacin using cyclic voltammetry, and the results showed that the PtNPs/KB/CD-MOF had high sensitivity, satisfactory stability and good reproducibility.
31990281	1	42	theme	Black	330:334	arg1	KB					337:338	Ketjen Black (KB) and platinum nanoparticles	323:366	KB	337:338	A facile and novel method was developed to improve the conductivity of a cyclodextrin-metal organic frameworks (CD-MOFs) by modifying it with Ketjen Black (KB) and platinum nanoparticles (PtNPs).
31990281	1	42	theme	Black	330:334	arg1	PtNPs					369:373	PtNPs	369:373	PtNPs	369:373	A facile and novel method was developed to improve the conductivity of a cyclodextrin-metal organic frameworks (CD-MOFs) by modifying it with Ketjen Black (KB) and platinum nanoparticles (PtNPs).
31990281	3	43	theme	satisfactory	823:834	arg1	stability					836:844	satisfactory stability	823:844	satisfactory stability	823:844	The sensor was then evaluated for its ability to detect ofloxacin using cyclic voltammetry, and the results showed that the PtNPs/KB/CD-MOF had high sensitivity, satisfactory stability and good reproducibility.
31990281	4	44	theme	detection	971:979	arg1	S/N					1000:1002	S/N = 3	1000:1006	S/N = 3	1000:1006	In addition, a great linear range was obtained with a concentration range of 0.08-100 μM and a low detection limit of 0.037 μM (S/N = 3).
31990281	4	44	theme	detection	971:979	arg1	limit					981:985	a low detection limit	965:985	a low detection limit of 0.037 μM (S/N = 3)	965:1007	In addition, a great linear range was obtained with a concentration range of 0.08-100 μM and a low detection limit of 0.037 μM (S/N = 3).
31990281	0	45	from	application	122:132	arg1	detection					157:165	the electrochemical detection	137:165	the electrochemical detection of ofloxacin	137:178	Facile synthesis of a cyclodextrin-metal organic framework decorated with Ketjen Black and platinum nanoparticles and its application in the electrochemical detection of ofloxacin.
32969233	4	0	theme	laboratory	551:560	arg1	COVID-19					600:607	laboratory, clinical, and radiological confirmed COVID-19	551:607	laboratory, clinical, and radiological confirmed COVID-19	551:607	Patients (n = 80) with laboratory, clinical, and radiological confirmed COVID-19 were included in this cross-sectional study.
32969233	6	1	theme	SPSS	795:798	arg1	version					800:806	SPSS version 26.0	795:811	SPSS version 26.0 (IBM)	795:817	Data were analyzed using SPSS version 26.0 (IBM).
32969233	6	1	theme	SPSS	795:798	arg1	IBM					814:816	IBM	814:816	IBM	814:816	Data were analyzed using SPSS version 26.0 (IBM).
32969233	9	2	theme	85	1297:1298	arg1	%					1299:1299	%	1299:1299	%	1299:1299	In the receiver operating characteristics curve analysis, we found that a serum endocan level of 276.4 ng/L had a 97% sensitivity and 85% specificity for prediction of the primary composite end point.
32969233	8	3	from	predictors	1106:1115	arg1	analysis					1023:1030	the logistic regression analysis	999:1030	the logistic regression analysis	999:1030	In the logistic regression analysis, only increased serum endocan levels and increase in age were independent predictors of the primary composite end point (P < .05).
32969233	4	4	theme	clinical	563:570	arg1	COVID-19					600:607	laboratory, clinical, and radiological confirmed COVID-19	551:607	laboratory, clinical, and radiological confirmed COVID-19	551:607	Patients (n = 80) with laboratory, clinical, and radiological confirmed COVID-19 were included in this cross-sectional study.
32969233	8	5	theme	independent	1094:1104	arg1	predictors					1106:1115	independent predictors	1094:1115	independent predictors of the primary composite end point (P < .05)	1094:1160	In the logistic regression analysis, only increased serum endocan levels and increase in age were independent predictors of the primary composite end point (P < .05).
32969233	4	6	theme	cross-sectional	631:645	arg1	study					647:651	this cross-sectional study	626:651	this cross-sectional study	626:651	Patients (n = 80) with laboratory, clinical, and radiological confirmed COVID-19 were included in this cross-sectional study.
32969233	1	7	theme	cardiovascular	159:172	arg1	system					174:179	the cardiovascular system	155:179	the cardiovascular system	155:179	Thrombotic and embolic complications in the cardiovascular system are evident and associated with worse prognosis in coronavirus disease 2019 (COVID-19) patients.
32969233	4	8	with	Patients	528:535	arg1	COVID-19					600:607	laboratory, clinical, and radiological confirmed COVID-19	551:607	laboratory, clinical, and radiological confirmed COVID-19	551:607	Patients (n = 80) with laboratory, clinical, and radiological confirmed COVID-19 were included in this cross-sectional study.
32969233	8	9	theme	primary	1124:1130	arg1	point					1146:1150	the primary composite end point	1120:1150	the primary composite end point (P < .05)	1120:1160	In the logistic regression analysis, only increased serum endocan levels and increase in age were independent predictors of the primary composite end point (P < .05).
32969233	8	9	theme	primary	1124:1130	arg1	<					1155:1155	P < .05	1153:1159	P < .05	1153:1159	In the logistic regression analysis, only increased serum endocan levels and increase in age were independent predictors of the primary composite end point (P < .05).
32969233	5	10	theme	blood	690:694	arg1	milliliter					658:667	Ten milliliter	654:667	Ten milliliter of peripheral venous blood	654:694	Ten milliliter of peripheral venous blood were drawn within 24 hours of admission to estimate serum endocan levels.
32969233	9	11	theme	composite	1343:1351	arg1	point					1357:1361	the primary composite end point	1331:1361	the primary composite end point	1331:1361	In the receiver operating characteristics curve analysis, we found that a serum endocan level of 276.4 ng/L had a 97% sensitivity and 85% specificity for prediction of the primary composite end point.
32969233	8	12	theme	end	1142:1144	arg1	point					1146:1150	the primary composite end point	1120:1150	the primary composite end point (P < .05)	1120:1160	In the logistic regression analysis, only increased serum endocan levels and increase in age were independent predictors of the primary composite end point (P < .05).
32969233	8	12	theme	end	1142:1144	arg1	<					1155:1155	P < .05	1153:1159	P < .05	1153:1159	In the logistic regression analysis, only increased serum endocan levels and increase in age were independent predictors of the primary composite end point (P < .05).
32969233	9	13	dep	sensitivity	1281:1291	arg1	a					1275:1275	a	1275:1275	a	1275:1275	In the receiver operating characteristics curve analysis, we found that a serum endocan level of 276.4 ng/L had a 97% sensitivity and 85% specificity for prediction of the primary composite end point.
32969233	9	14	theme	point	1357:1361	arg1	prediction					1317:1326	prediction	1317:1326	prediction of the primary composite end point	1317:1361	In the receiver operating characteristics curve analysis, we found that a serum endocan level of 276.4 ng/L had a 97% sensitivity and 85% specificity for prediction of the primary composite end point.
32969233	5	15	theme	peripheral	672:681	arg1	blood					690:694	peripheral venous blood	672:694	peripheral venous blood	672:694	Ten milliliter of peripheral venous blood were drawn within 24 hours of admission to estimate serum endocan levels.
32969233	1	16	theme	Thrombotic	115:124	arg1	complications					138:150	Thrombotic and embolic complications	115:150	Thrombotic and embolic complications in the cardiovascular system	115:179	Thrombotic and embolic complications in the cardiovascular system are evident and associated with worse prognosis in coronavirus disease 2019 (COVID-19) patients.
32969233	0	17	theme	Worse	54:58	arg1	Outcomes					69:76	Worse Clinical Outcomes	54:76	Worse Clinical Outcomes in COVID-19 Patients: A Pilot Study	54:112	Serum Endocan Levels on Admission Are Associated With Worse Clinical Outcomes in COVID-19 Patients: A Pilot Study.
32969233	1	18	theme	embolic	130:136	arg1	complications					138:150	Thrombotic and embolic complications	115:150	Thrombotic and embolic complications in the cardiovascular system	115:179	Thrombotic and embolic complications in the cardiovascular system are evident and associated with worse prognosis in coronavirus disease 2019 (COVID-19) patients.
32969233	0	19	from	Levels	14:19	arg1	Admission					24:32	Admission	24:32	Admission	24:32	Serum Endocan Levels on Admission Are Associated With Worse Clinical Outcomes in COVID-19 Patients: A Pilot Study.
32969233	1	20	from	prognosis	219:227	arg1	patients					268:275	coronavirus disease 2019 (COVID-19) patients	232:275	coronavirus disease 2019 (COVID-19) patients	232:275	Thrombotic and embolic complications in the cardiovascular system are evident and associated with worse prognosis in coronavirus disease 2019 (COVID-19) patients.
32969233	5	21	theme	serum	748:752	arg1	levels					762:767	serum endocan levels	748:767	serum endocan levels	748:767	Ten milliliter of peripheral venous blood were drawn within 24 hours of admission to estimate serum endocan levels.
32969233	9	22	theme	curve	1205:1209	arg1	analysis					1211:1218	characteristics curve analysis	1189:1218	characteristics curve analysis	1189:1218	In the receiver operating characteristics curve analysis, we found that a serum endocan level of 276.4 ng/L had a 97% sensitivity and 85% specificity for prediction of the primary composite end point.
32969233	1	23	from	complications	138:150	arg1	system					174:179	the cardiovascular system	155:179	the cardiovascular system	155:179	Thrombotic and embolic complications in the cardiovascular system are evident and associated with worse prognosis in coronavirus disease 2019 (COVID-19) patients.
32969233	7	24	contain	had	866:868	arg1	Patients					820:827	Patients	820:827	Patients with the primary composite end point	820:864	Patients with the primary composite end point had significantly higher serum endocan levels than patients without (852.2 ± 522.7 vs 550.2 ± 440.8 ng/L, respectively; P < .01).
32969233	7	24	contain	had	866:868	arg2	levels					905:910	significantly higher serum endocan levels	870:910	significantly higher serum endocan levels	870:910	Patients with the primary composite end point had significantly higher serum endocan levels than patients without (852.2 ± 522.7 vs 550.2 ± 440.8 ng/L, respectively; P < .01).
32969233	3	25	theme	primary	426:432	arg1	point					448:452	primary composite end point	426:452	primary composite end point (mortality and intensive care unit hospitalization) in COVID-19 patients	426:525	We hypothesized serum endocan levels on admission are associated with primary composite end point (mortality and intensive care unit hospitalization) in COVID-19 patients.
32969233	3	26	theme	serum	372:376	arg1	levels					386:391	serum endocan levels	372:391	serum endocan levels	372:391	We hypothesized serum endocan levels on admission are associated with primary composite end point (mortality and intensive care unit hospitalization) in COVID-19 patients.
32969233	0	27	theme	Clinical	60:67	arg1	Outcomes					69:76	Worse Clinical Outcomes	54:76	Worse Clinical Outcomes in COVID-19 Patients: A Pilot Study	54:112	Serum Endocan Levels on Admission Are Associated With Worse Clinical Outcomes in COVID-19 Patients: A Pilot Study.
32969233	0	28	theme	Serum	0:4	arg1	Levels					14:19	Serum Endocan Levels	0:19	Serum Endocan Levels on Admission	0:32	Serum Endocan Levels on Admission Are Associated With Worse Clinical Outcomes in COVID-19 Patients: A Pilot Study.
32969233	7	29	theme	serum	891:895	arg1	levels					905:910	significantly higher serum endocan levels	870:910	significantly higher serum endocan levels	870:910	Patients with the primary composite end point had significantly higher serum endocan levels than patients without (852.2 ± 522.7 vs 550.2 ± 440.8 ng/L, respectively; P < .01).
32969233	0	30	dep	Outcomes	69:76	arg1	Study					108:112	A Pilot Study	100:112	Worse Clinical Outcomes in COVID-19 Patients: A Pilot Study	54:112	Serum Endocan Levels on Admission Are Associated With Worse Clinical Outcomes in COVID-19 Patients: A Pilot Study.
32969233	10	31	theme	endocan	1379:1385	arg1	levels					1387:1392	Baseline serum endocan levels	1364:1392	Baseline serum endocan levels	1364:1392	Baseline serum endocan levels may prove useful as a prognostic factor in patients hospitalized for COVID-19.
32969233	9	32	theme	endocan	1243:1249	arg1	level					1251:1255	a serum endocan level	1235:1255	a serum endocan level of 276.4 ng/L	1235:1269	In the receiver operating characteristics curve analysis, we found that a serum endocan level of 276.4 ng/L had a 97% sensitivity and 85% specificity for prediction of the primary composite end point.
32969233	8	33	from	increase	1073:1080	arg1	age					1085:1087	age	1085:1087	age	1085:1087	In the logistic regression analysis, only increased serum endocan levels and increase in age were independent predictors of the primary composite end point (P < .05).
32969233	10	34	theme	Baseline	1364:1371	arg1	levels					1387:1392	Baseline serum endocan levels	1364:1392	Baseline serum endocan levels	1364:1392	Baseline serum endocan levels may prove useful as a prognostic factor in patients hospitalized for COVID-19.
32969233	3	35	theme	end	444:446	arg1	point					448:452	primary composite end point	426:452	primary composite end point (mortality and intensive care unit hospitalization) in COVID-19 patients	426:525	We hypothesized serum endocan levels on admission are associated with primary composite end point (mortality and intensive care unit hospitalization) in COVID-19 patients.
32969233	8	36	theme	regression	1012:1021	arg1	analysis					1023:1030	the logistic regression analysis	999:1030	the logistic regression analysis	999:1030	In the logistic regression analysis, only increased serum endocan levels and increase in age were independent predictors of the primary composite end point (P < .05).
32969233	8	37	from	levels	1062:1067	arg1	age					1085:1087	age	1085:1087	age	1085:1087	In the logistic regression analysis, only increased serum endocan levels and increase in age were independent predictors of the primary composite end point (P < .05).
32969233	3	38	from	point	448:452	arg1	patients					518:525	COVID-19 patients	509:525	COVID-19 patients	509:525	We hypothesized serum endocan levels on admission are associated with primary composite end point (mortality and intensive care unit hospitalization) in COVID-19 patients.
32969233	3	39	theme	care	479:482	arg1	unit					484:487	intensive care unit	469:487	intensive care unit hospitalization	469:503	We hypothesized serum endocan levels on admission are associated with primary composite end point (mortality and intensive care unit hospitalization) in COVID-19 patients.
32969233	9	40	theme	ng/L	1266:1269	arg1	level					1251:1255	a serum endocan level	1235:1255	a serum endocan level of 276.4 ng/L	1235:1269	In the receiver operating characteristics curve analysis, we found that a serum endocan level of 276.4 ng/L had a 97% sensitivity and 85% specificity for prediction of the primary composite end point.
32969233	10	41	from	factor	1427:1432	arg1	patients					1437:1444	patients	1437:1444	patients hospitalized for COVID-19	1437:1470	Baseline serum endocan levels may prove useful as a prognostic factor in patients hospitalized for COVID-19.
32969233	7	42	theme	higher	884:889	arg1	levels					905:910	significantly higher serum endocan levels	870:910	significantly higher serum endocan levels	870:910	Patients with the primary composite end point had significantly higher serum endocan levels than patients without (852.2 ± 522.7 vs 550.2 ± 440.8 ng/L, respectively; P < .01).
32969233	1	43	theme	worse	213:217	arg1	prognosis					219:227	worse prognosis	213:227	worse prognosis in coronavirus disease 2019 (COVID-19) patients	213:275	Thrombotic and embolic complications in the cardiovascular system are evident and associated with worse prognosis in coronavirus disease 2019 (COVID-19) patients.
32969233	8	44	theme	increased	1038:1046	arg1	levels					1062:1067	only increased serum endocan levels	1033:1067	only increased serum endocan levels	1033:1067	In the logistic regression analysis, only increased serum endocan levels and increase in age were independent predictors of the primary composite end point (P < .05).
32969233	7	45	with	Patients	820:827	arg1	point					860:864	the primary composite end point	834:864	the primary composite end point	834:864	Patients with the primary composite end point had significantly higher serum endocan levels than patients without (852.2 ± 522.7 vs 550.2 ± 440.8 ng/L, respectively; P < .01).
32969233	3	46	dep	point	448:452	arg1	hospitalization					489:503	intensive care unit hospitalization	469:503	intensive care unit hospitalization	469:503	We hypothesized serum endocan levels on admission are associated with primary composite end point (mortality and intensive care unit hospitalization) in COVID-19 patients.
32969233	3	46	dep	point	448:452	arg1	mortality					455:463	mortality	455:463	mortality	455:463	We hypothesized serum endocan levels on admission are associated with primary composite end point (mortality and intensive care unit hospitalization) in COVID-19 patients.
32969233	9	47	theme	%	1279:1279	arg1	sensitivity					1281:1291	97% sensitivity	1277:1291	97% sensitivity	1277:1291	In the receiver operating characteristics curve analysis, we found that a serum endocan level of 276.4 ng/L had a 97% sensitivity and 85% specificity for prediction of the primary composite end point.
32969233	8	48	theme	endocan	1054:1060	arg1	levels					1062:1067	only increased serum endocan levels	1033:1067	only increased serum endocan levels	1033:1067	In the logistic regression analysis, only increased serum endocan levels and increase in age were independent predictors of the primary composite end point (P < .05).
32969233	4	49	theme	radiological	577:588	arg1	COVID-19					600:607	laboratory, clinical, and radiological confirmed COVID-19	551:607	laboratory, clinical, and radiological confirmed COVID-19	551:607	Patients (n = 80) with laboratory, clinical, and radiological confirmed COVID-19 were included in this cross-sectional study.
32969233	2	50	theme	vascular	336:343	arg1	pathology					345:353	vascular pathology	336:353	vascular pathology	336:353	Endothelial-specific molecule 1 (endocan) plays a role in vascular pathology.
32969233	7	51	theme	end	856:858	arg1	point					860:864	the primary composite end point	834:864	the primary composite end point	834:864	Patients with the primary composite end point had significantly higher serum endocan levels than patients without (852.2 ± 522.7 vs 550.2 ± 440.8 ng/L, respectively; P < .01).
32969233	1	52	theme	disease	244:250	arg1	patients					268:275	coronavirus disease 2019 (COVID-19) patients	232:275	coronavirus disease 2019 (COVID-19) patients	232:275	Thrombotic and embolic complications in the cardiovascular system are evident and associated with worse prognosis in coronavirus disease 2019 (COVID-19) patients.
32969233	2	53	theme	Endothelial-specific	278:297	arg1	molecule					299:306	Endothelial-specific molecule 1	278:308	Endothelial-specific molecule 1 (endocan)	278:318	Endothelial-specific molecule 1 (endocan) plays a role in vascular pathology.
32969233	2	53	theme	Endothelial-specific	278:297	arg1	endocan					311:317	endocan	311:317	endocan	311:317	Endothelial-specific molecule 1 (endocan) plays a role in vascular pathology.
32969233	7	54	theme	primary	838:844	arg1	point					860:864	the primary composite end point	834:864	the primary composite end point	834:864	Patients with the primary composite end point had significantly higher serum endocan levels than patients without (852.2 ± 522.7 vs 550.2 ± 440.8 ng/L, respectively; P < .01).
32969233	9	55	theme	%	1299:1299	arg1	specificity					1301:1311	85% specificity	1297:1311	85% specificity	1297:1311	In the receiver operating characteristics curve analysis, we found that a serum endocan level of 276.4 ng/L had a 97% sensitivity and 85% specificity for prediction of the primary composite end point.
32969233	8	56	theme	P	1153:1153	arg1	point					1146:1150	the primary composite end point	1120:1150	the primary composite end point (P < .05)	1120:1160	In the logistic regression analysis, only increased serum endocan levels and increase in age were independent predictors of the primary composite end point (P < .05).
32969233	8	56	theme	P	1153:1153	arg1	<					1155:1155	P < .05	1153:1159	P < .05	1153:1159	In the logistic regression analysis, only increased serum endocan levels and increase in age were independent predictors of the primary composite end point (P < .05).
32969233	5	57	theme	venous	683:688	arg1	blood					690:694	peripheral venous blood	672:694	peripheral venous blood	672:694	Ten milliliter of peripheral venous blood were drawn within 24 hours of admission to estimate serum endocan levels.
32969233	9	58	theme	primary	1335:1341	arg1	point					1357:1361	the primary composite end point	1331:1361	the primary composite end point	1331:1361	In the receiver operating characteristics curve analysis, we found that a serum endocan level of 276.4 ng/L had a 97% sensitivity and 85% specificity for prediction of the primary composite end point.
32969233	9	59	theme	end	1353:1355	arg1	point					1357:1361	the primary composite end point	1331:1361	the primary composite end point	1331:1361	In the receiver operating characteristics curve analysis, we found that a serum endocan level of 276.4 ng/L had a 97% sensitivity and 85% specificity for prediction of the primary composite end point.
32969233	8	60	theme	composite	1132:1140	arg1	point					1146:1150	the primary composite end point	1120:1150	the primary composite end point (P < .05)	1120:1160	In the logistic regression analysis, only increased serum endocan levels and increase in age were independent predictors of the primary composite end point (P < .05).
32969233	8	60	theme	composite	1132:1140	arg1	<					1155:1155	P < .05	1153:1159	P < .05	1153:1159	In the logistic regression analysis, only increased serum endocan levels and increase in age were independent predictors of the primary composite end point (P < .05).
32969233	8	61	theme	point	1146:1150	arg1	predictors					1106:1115	independent predictors	1094:1115	independent predictors of the primary composite end point (P < .05)	1094:1160	In the logistic regression analysis, only increased serum endocan levels and increase in age were independent predictors of the primary composite end point (P < .05).
32969233	5	62	theme	admission	726:734	arg1	hours					717:721	24 hours	714:721	24 hours of admission	714:734	Ten milliliter of peripheral venous blood were drawn within 24 hours of admission to estimate serum endocan levels.
32969233	9	63	theme	characteristics	1189:1203	arg1	analysis					1211:1218	characteristics curve analysis	1189:1218	characteristics curve analysis	1189:1218	In the receiver operating characteristics curve analysis, we found that a serum endocan level of 276.4 ng/L had a 97% sensitivity and 85% specificity for prediction of the primary composite end point.
32969233	0	64	theme	Pilot	102:106	arg1	Study					108:112	A Pilot Study	100:112	Worse Clinical Outcomes in COVID-19 Patients: A Pilot Study	54:112	Serum Endocan Levels on Admission Are Associated With Worse Clinical Outcomes in COVID-19 Patients: A Pilot Study.
32969233	7	65	dep	±	958:958	arg1	P					986:986	P < .01	986:992	P < .01	986:992	Patients with the primary composite end point had significantly higher serum endocan levels than patients without (852.2 ± 522.7 vs 550.2 ± 440.8 ng/L, respectively; P < .01).
32969233	0	66	theme	A	100:100	arg1	Study					108:112	A Pilot Study	100:112	Worse Clinical Outcomes in COVID-19 Patients: A Pilot Study	54:112	Serum Endocan Levels on Admission Are Associated With Worse Clinical Outcomes in COVID-19 Patients: A Pilot Study.
32969233	3	67	theme	composite	434:442	arg1	point					448:452	primary composite end point	426:452	primary composite end point (mortality and intensive care unit hospitalization) in COVID-19 patients	426:525	We hypothesized serum endocan levels on admission are associated with primary composite end point (mortality and intensive care unit hospitalization) in COVID-19 patients.
32969233	0	68	theme	Endocan	6:12	arg1	Levels					14:19	Serum Endocan Levels	0:19	Serum Endocan Levels on Admission	0:32	Serum Endocan Levels on Admission Are Associated With Worse Clinical Outcomes in COVID-19 Patients: A Pilot Study.
32969233	3	69	theme	endocan	378:384	arg1	levels					386:391	serum endocan levels	372:391	serum endocan levels	372:391	We hypothesized serum endocan levels on admission are associated with primary composite end point (mortality and intensive care unit hospitalization) in COVID-19 patients.
32969233	4	70	theme	=	540:540	arg1	Patients					528:535	Patients	528:535	Patients (n = 80) with laboratory, clinical, and radiological confirmed COVID-19	528:607	Patients (n = 80) with laboratory, clinical, and radiological confirmed COVID-19 were included in this cross-sectional study.
32969233	4	70	theme	=	540:540	arg1	n					538:538	n = 80	538:543	n = 80	538:543	Patients (n = 80) with laboratory, clinical, and radiological confirmed COVID-19 were included in this cross-sectional study.
32969233	0	71	from	Outcomes	69:76	arg1	Patients					90:97	COVID-19 Patients	81:97	COVID-19 Patients	81:97	Serum Endocan Levels on Admission Are Associated With Worse Clinical Outcomes in COVID-19 Patients: A Pilot Study.
32969233	9	72	theme	serum	1237:1241	arg1	level					1251:1255	a serum endocan level	1235:1255	a serum endocan level of 276.4 ng/L	1235:1269	In the receiver operating characteristics curve analysis, we found that a serum endocan level of 276.4 ng/L had a 97% sensitivity and 85% specificity for prediction of the primary composite end point.
32969233	8	73	theme	logistic	1003:1010	arg1	analysis					1023:1030	the logistic regression analysis	999:1030	the logistic regression analysis	999:1030	In the logistic regression analysis, only increased serum endocan levels and increase in age were independent predictors of the primary composite end point (P < .05).
32969233	7	74	theme	endocan	897:903	arg1	levels					905:910	significantly higher serum endocan levels	870:910	significantly higher serum endocan levels	870:910	Patients with the primary composite end point had significantly higher serum endocan levels than patients without (852.2 ± 522.7 vs 550.2 ± 440.8 ng/L, respectively; P < .01).
32969233	10	75	theme	serum	1373:1377	arg1	levels					1387:1392	Baseline serum endocan levels	1364:1392	Baseline serum endocan levels	1364:1392	Baseline serum endocan levels may prove useful as a prognostic factor in patients hospitalized for COVID-19.
32969233	5	76	theme	endocan	754:760	arg1	levels					762:767	serum endocan levels	748:767	serum endocan levels	748:767	Ten milliliter of peripheral venous blood were drawn within 24 hours of admission to estimate serum endocan levels.
32969233	3	77	theme	unit	484:487	arg1	hospitalization					489:503	intensive care unit hospitalization	469:503	intensive care unit hospitalization	469:503	We hypothesized serum endocan levels on admission are associated with primary composite end point (mortality and intensive care unit hospitalization) in COVID-19 patients.
32969233	8	78	theme	serum	1048:1052	arg1	levels					1062:1067	only increased serum endocan levels	1033:1067	only increased serum endocan levels	1033:1067	In the logistic regression analysis, only increased serum endocan levels and increase in age were independent predictors of the primary composite end point (P < .05).
32969233	9	79	contain	had	1271:1273	arg2	specificity					1301:1311	85% specificity	1297:1311	85% specificity	1297:1311	In the receiver operating characteristics curve analysis, we found that a serum endocan level of 276.4 ng/L had a 97% sensitivity and 85% specificity for prediction of the primary composite end point.
32969233	9	79	contain	had	1271:1273	arg1	level					1251:1255	a serum endocan level	1235:1255	a serum endocan level of 276.4 ng/L	1235:1269	In the receiver operating characteristics curve analysis, we found that a serum endocan level of 276.4 ng/L had a 97% sensitivity and 85% specificity for prediction of the primary composite end point.
32969233	9	79	contain	had	1271:1273	arg2	sensitivity					1281:1291	97% sensitivity	1277:1291	97% sensitivity	1277:1291	In the receiver operating characteristics curve analysis, we found that a serum endocan level of 276.4 ng/L had a 97% sensitivity and 85% specificity for prediction of the primary composite end point.
32969233	3	80	theme	intensive	469:477	arg1	unit					484:487	intensive care unit	469:487	intensive care unit hospitalization	469:503	We hypothesized serum endocan levels on admission are associated with primary composite end point (mortality and intensive care unit hospitalization) in COVID-19 patients.
32969233	9	81	theme	97	1277:1278	arg1	%					1279:1279	%	1279:1279	%	1279:1279	In the receiver operating characteristics curve analysis, we found that a serum endocan level of 276.4 ng/L had a 97% sensitivity and 85% specificity for prediction of the primary composite end point.
32969233	4	82	theme	confirmed	590:598	arg1	COVID-19					600:607	laboratory, clinical, and radiological confirmed COVID-19	551:607	laboratory, clinical, and radiological confirmed COVID-19	551:607	Patients (n = 80) with laboratory, clinical, and radiological confirmed COVID-19 were included in this cross-sectional study.
32969233	10	83	theme	prognostic	1416:1425	arg1	factor					1427:1432	a prognostic factor	1414:1432	a prognostic factor in patients hospitalized for COVID-19	1414:1470	Baseline serum endocan levels may prove useful as a prognostic factor in patients hospitalized for COVID-19.
32969233	3	84	theme	COVID-19	509:516	arg1	patients					518:525	COVID-19 patients	509:525	COVID-19 patients	509:525	We hypothesized serum endocan levels on admission are associated with primary composite end point (mortality and intensive care unit hospitalization) in COVID-19 patients.
32969233	7	85	theme	composite	846:854	arg1	point					860:864	the primary composite end point	834:864	the primary composite end point	834:864	Patients with the primary composite end point had significantly higher serum endocan levels than patients without (852.2 ± 522.7 vs 550.2 ± 440.8 ng/L, respectively; P < .01).
32969233	1	86	theme	coronavirus	232:242	arg1	disease					244:250	coronavirus disease 2019	232:255	coronavirus disease 2019 (COVID-19) patients	232:275	Thrombotic and embolic complications in the cardiovascular system are evident and associated with worse prognosis in coronavirus disease 2019 (COVID-19) patients.
32969233	1	86	theme	coronavirus	232:242	arg1	COVID-19					258:265	COVID-19	258:265	COVID-19	258:265	Thrombotic and embolic complications in the cardiovascular system are evident and associated with worse prognosis in coronavirus disease 2019 (COVID-19) patients.
32841769	0	0	theme	tandem	105:110	arg1	spectrometry					117:128	liquid chromatography tandem mass spectrometry	83:128	liquid chromatography tandem mass spectrometry	83:128	Optimizing sample preparation workflow for bioanalysis of oligonucleotides through liquid chromatography tandem mass spectrometry.
32841769	6	1	theme	sample	899:904	arg1	procedure					914:922	the most efficient sample cleanup procedure	880:922	the most efficient sample cleanup procedure with low cost and less toxicity	880:954	LLE with phenol: dichloromethane (2:1, v:v) was found to be the most efficient sample cleanup procedure with low cost and less toxicity.
32841769	6	1	theme	sample	899:904	arg1	dichloromethane					837:851	dichloromethane	837:851	dichloromethane (2:1, v:v)	837:862	LLE with phenol: dichloromethane (2:1, v:v) was found to be the most efficient sample cleanup procedure with low cost and less toxicity.
32841769	5	2	theme	liquid-liquid	703:715	arg1	extraction					717:726	liquid-liquid extraction	703:726	liquid-liquid extraction (LLE)	703:732	These included liquid-liquid extraction (LLE), solid-phase extraction (SPE), protein precipitation (PPT) and LLE combined with SPE.
32841769	5	2	theme	liquid-liquid	703:715	arg1	LLE					729:731	LLE	729:731	LLE	729:731	These included liquid-liquid extraction (LLE), solid-phase extraction (SPE), protein precipitation (PPT) and LLE combined with SPE.
32841769	12	3	theme	Matrix	1481:1486	arg1	effect					1488:1493	Matrix effect	1481:1493	Matrix effect	1481:1493	Matrix effect and carryover were also evaluated and no significant effect was observed.
32841769	0	4	theme	chromatography	90:103	arg1	spectrometry					117:128	liquid chromatography tandem mass spectrometry	83:128	liquid chromatography tandem mass spectrometry	83:128	Optimizing sample preparation workflow for bioanalysis of oligonucleotides through liquid chromatography tandem mass spectrometry.
32841769	6	5	theme	efficient	889:897	arg1	procedure					914:922	the most efficient sample cleanup procedure	880:922	the most efficient sample cleanup procedure with low cost and less toxicity	880:954	LLE with phenol: dichloromethane (2:1, v:v) was found to be the most efficient sample cleanup procedure with low cost and less toxicity.
32841769	6	5	theme	efficient	889:897	arg1	dichloromethane					837:851	dichloromethane	837:851	dichloromethane (2:1, v:v)	837:862	LLE with phenol: dichloromethane (2:1, v:v) was found to be the most efficient sample cleanup procedure with low cost and less toxicity.
32841769	5	6	theme	protein	765:771	arg1	PPT					788:790	PPT	788:790	PPT	788:790	These included liquid-liquid extraction (LLE), solid-phase extraction (SPE), protein precipitation (PPT) and LLE combined with SPE.
32841769	5	6	theme	protein	765:771	arg1	precipitation					773:785	protein precipitation	765:785	protein precipitation (PPT)	765:791	These included liquid-liquid extraction (LLE), solid-phase extraction (SPE), protein precipitation (PPT) and LLE combined with SPE.
32841769	9	7	theme	central	1167:1173	arg1	suite					1192:1196	the central composite design suite	1163:1196	the central composite design suite	1163:1196	It was found that the central composite design suite was proved to be highly practical for optimizing MS parameters.
32841769	9	7	theme	central	1167:1173	arg1	practical					1222:1230	practical	1222:1230	practical	1222:1230	It was found that the central composite design suite was proved to be highly practical for optimizing MS parameters.
32841769	4	8	contain	containing	571:580	arg2	oligonucleotide					591:605	a 16 mer oligonucleotide standard	582:614	a 16 mer oligonucleotide standard	582:614	In this study, we have employed a model formed with rat plasma containing a 16 mer oligonucleotide standard in order to comprehensively optimize the sample preparation procedures.
32841769	4	8	contain	containing	571:580	arg1	plasma					564:569	rat plasma	560:569	rat plasma containing a 16 mer oligonucleotide standard	560:614	In this study, we have employed a model formed with rat plasma containing a 16 mer oligonucleotide standard in order to comprehensively optimize the sample preparation procedures.
32841769	0	9	theme	mass	112:115	arg1	spectrometry					117:128	liquid chromatography tandem mass spectrometry	83:128	liquid chromatography tandem mass spectrometry	83:128	Optimizing sample preparation workflow for bioanalysis of oligonucleotides through liquid chromatography tandem mass spectrometry.
32841769	10	10	theme	preparation	1303:1313	arg1	workflow					1315:1322	the thoroughly optimized sample preparation workflow	1271:1322	the thoroughly optimized sample preparation workflow	1271:1322	Finally, the thoroughly optimized sample preparation workflow was fully validated.
32841769	2	11	theme	approach	320:327	arg1	lack					276:279	lack	276:279	lack of simple and efficient sample cleanup approach	276:327	However, lack of simple and efficient sample cleanup approach remains a challenge.
32841769	7	12	theme	drying	1056:1061	arg1	conditions					1063:1072	the optimal drying conditions	1044:1072	the optimal drying conditions	1044:1072	Followed by the extraction, ethanol precipitation (-80 °C, 5 min) was determined to be the optimal drying conditions.
32841769	7	12	theme	drying	1056:1061	arg1	precipitation					993:1005	ethanol precipitation	985:1005	ethanol precipitation (-80 °C, 5 min)	985:1021	Followed by the extraction, ethanol precipitation (-80 °C, 5 min) was determined to be the optimal drying conditions.
32841769	2	13	theme	cleanup	312:318	arg1	approach					320:327	simple and efficient sample cleanup approach	284:327	simple and efficient sample cleanup approach	284:327	However, lack of simple and efficient sample cleanup approach remains a challenge.
32841769	1	14	theme	used	196:199	arg1	spectrometry					165:176	Liquid chromatography tandem mass spectrometry	131:176	Liquid chromatography tandem mass spectrometry	131:176	Liquid chromatography tandem mass spectrometry has been a widely used technique for quantifying oligonucleotides in biological samples.
32841769	1	14	theme	used	196:199	arg1	technique					201:209	a widely used technique	187:209	a widely used technique for quantifying oligonucleotides in biological samples	187:264	Liquid chromatography tandem mass spectrometry has been a widely used technique for quantifying oligonucleotides in biological samples.
32841769	3	15	theme	major	382:386	arg1	factors					388:394	the major factors	378:394	the major factors	378:394	Our study aimed to evaluate the major factors during the sample pretreatment process for developing optimal sample preparation workflow for oligonucleotides.
32841769	2	16	theme	sample	305:310	arg1	approach					320:327	simple and efficient sample cleanup approach	284:327	simple and efficient sample cleanup approach	284:327	However, lack of simple and efficient sample cleanup approach remains a challenge.
32841769	6	17	dep	LLE	820:822	arg1	found					868:872	found	868:872	was found to be the most efficient sample cleanup procedure with low cost and less toxicity	864:954	LLE with phenol: dichloromethane (2:1, v:v) was found to be the most efficient sample cleanup procedure with low cost and less toxicity.
32841769	0	18	theme	sample	11:16	arg1	preparation					18:28	sample preparation	11:28	sample preparation	11:28	Optimizing sample preparation workflow for bioanalysis of oligonucleotides through liquid chromatography tandem mass spectrometry.
32841769	11	19	theme	developed	1349:1357	arg1	assay					1359:1363	The developed assay	1345:1363	The developed assay	1345:1363	The developed assay provided a quantitative range of 0.25-1000 nM, with accuracy and precision were < 7.45% and < 12.20%, respectively.
32841769	6	20	dep	2:1	854:856	arg1	v					861:861	v	861:861	v	861:861	LLE with phenol: dichloromethane (2:1, v:v) was found to be the most efficient sample cleanup procedure with low cost and less toxicity.
32841769	4	21	theme	mer	587:589	arg1	oligonucleotide					591:605	a 16 mer oligonucleotide standard	582:614	a 16 mer oligonucleotide standard	582:614	In this study, we have employed a model formed with rat plasma containing a 16 mer oligonucleotide standard in order to comprehensively optimize the sample preparation procedures.
32841769	7	22	theme	optimal	1048:1054	arg1	conditions					1063:1072	the optimal drying conditions	1044:1072	the optimal drying conditions	1044:1072	Followed by the extraction, ethanol precipitation (-80 °C, 5 min) was determined to be the optimal drying conditions.
32841769	7	22	theme	optimal	1048:1054	arg1	precipitation					993:1005	ethanol precipitation	985:1005	ethanol precipitation (-80 °C, 5 min)	985:1021	Followed by the extraction, ethanol precipitation (-80 °C, 5 min) was determined to be the optimal drying conditions.
32841769	9	23	theme	composite	1175:1183	arg1	suite					1192:1196	the central composite design suite	1163:1196	the central composite design suite	1163:1196	It was found that the central composite design suite was proved to be highly practical for optimizing MS parameters.
32841769	9	23	theme	composite	1175:1183	arg1	practical					1222:1230	practical	1222:1230	practical	1222:1230	It was found that the central composite design suite was proved to be highly practical for optimizing MS parameters.
32841769	6	24	with	procedure	914:922	arg1	cost					933:936	low cost	929:936	low cost	929:936	LLE with phenol: dichloromethane (2:1, v:v) was found to be the most efficient sample cleanup procedure with low cost and less toxicity.
32841769	6	24	with	procedure	914:922	arg1	toxicity					947:954	less toxicity	942:954	less toxicity	942:954	LLE with phenol: dichloromethane (2:1, v:v) was found to be the most efficient sample cleanup procedure with low cost and less toxicity.
32841769	3	25	theme	workflow	477:484	arg1	preparation					465:475	optimal sample preparation	450:475	optimal sample preparation workflow for oligonucleotides	450:505	Our study aimed to evaluate the major factors during the sample pretreatment process for developing optimal sample preparation workflow for oligonucleotides.
32841769	9	26	theme	design	1185:1190	arg1	suite					1192:1196	the central composite design suite	1163:1196	the central composite design suite	1163:1196	It was found that the central composite design suite was proved to be highly practical for optimizing MS parameters.
32841769	9	26	theme	design	1185:1190	arg1	practical					1222:1230	practical	1222:1230	practical	1222:1230	It was found that the central composite design suite was proved to be highly practical for optimizing MS parameters.
32841769	11	27	theme	<	1445:1445	arg1	%					1451:1451	< 7.45%	1445:1451	< 7.45%	1445:1451	The developed assay provided a quantitative range of 0.25-1000 nM, with accuracy and precision were < 7.45% and < 12.20%, respectively.
32841769	3	28	theme	sample	407:412	arg1	process					427:433	the sample pretreatment process	403:433	the sample pretreatment process for developing optimal sample preparation workflow for oligonucleotides	403:505	Our study aimed to evaluate the major factors during the sample pretreatment process for developing optimal sample preparation workflow for oligonucleotides.
32841769	4	29	theme	standard	607:614	arg1	oligonucleotide					591:605	a 16 mer oligonucleotide standard	582:614	a 16 mer oligonucleotide standard	582:614	In this study, we have employed a model formed with rat plasma containing a 16 mer oligonucleotide standard in order to comprehensively optimize the sample preparation procedures.
32841769	10	30	theme	sample	1296:1301	arg1	workflow					1315:1322	the thoroughly optimized sample preparation workflow	1271:1322	the thoroughly optimized sample preparation workflow	1271:1322	Finally, the thoroughly optimized sample preparation workflow was fully validated.
32841769	1	31	theme	Liquid	131:136	arg1	spectrometry					165:176	Liquid chromatography tandem mass spectrometry	131:176	Liquid chromatography tandem mass spectrometry	131:176	Liquid chromatography tandem mass spectrometry has been a widely used technique for quantifying oligonucleotides in biological samples.
32841769	1	31	theme	Liquid	131:136	arg1	technique					201:209	a widely used technique	187:209	a widely used technique for quantifying oligonucleotides in biological samples	187:264	Liquid chromatography tandem mass spectrometry has been a widely used technique for quantifying oligonucleotides in biological samples.
32841769	6	32	dep	dichloromethane	837:851	arg1	v					859:859	v	859:859	v	859:859	LLE with phenol: dichloromethane (2:1, v:v) was found to be the most efficient sample cleanup procedure with low cost and less toxicity.
32841769	6	32	dep	dichloromethane	837:851	arg1	2:1					854:856	2:1	854:856	2:1	854:856	LLE with phenol: dichloromethane (2:1, v:v) was found to be the most efficient sample cleanup procedure with low cost and less toxicity.
32841769	3	33	theme	optimal	450:456	arg1	preparation					465:475	optimal sample preparation	450:475	optimal sample preparation workflow for oligonucleotides	450:505	Our study aimed to evaluate the major factors during the sample pretreatment process for developing optimal sample preparation workflow for oligonucleotides.
32841769	1	34	theme	biological	247:256	arg1	samples					258:264	biological samples	247:264	biological samples	247:264	Liquid chromatography tandem mass spectrometry has been a widely used technique for quantifying oligonucleotides in biological samples.
32841769	3	35	theme	sample	458:463	arg1	preparation					465:475	optimal sample preparation	450:475	optimal sample preparation workflow for oligonucleotides	450:505	Our study aimed to evaluate the major factors during the sample pretreatment process for developing optimal sample preparation workflow for oligonucleotides.
32841769	11	36	theme	quantitative	1376:1387	arg1	range					1389:1393	a quantitative range	1374:1393	a quantitative range of 0.25-1000 nM	1374:1409	The developed assay provided a quantitative range of 0.25-1000 nM, with accuracy and precision were < 7.45% and < 12.20%, respectively.
32841769	0	37	theme	oligonucleotides	58:73	arg1	bioanalysis					43:53	bioanalysis	43:53	bioanalysis of oligonucleotides	43:73	Optimizing sample preparation workflow for bioanalysis of oligonucleotides through liquid chromatography tandem mass spectrometry.
32841769	4	38	theme	rat	560:562	arg1	plasma					564:569	rat plasma	560:569	rat plasma containing a 16 mer oligonucleotide standard	560:614	In this study, we have employed a model formed with rat plasma containing a 16 mer oligonucleotide standard in order to comprehensively optimize the sample preparation procedures.
32841769	8	39	theme	spectrometric	1086:1098	arg1	parameters					1100:1109	mass spectrometric parameters	1081:1109	mass spectrometric parameters	1081:1109	Also, mass spectrometric parameters were tuned to optimal conditions.
32841769	8	40	theme	mass	1081:1084	arg1	parameters					1100:1109	mass spectrometric parameters	1081:1109	mass spectrometric parameters	1081:1109	Also, mass spectrometric parameters were tuned to optimal conditions.
32841769	1	41	theme	chromatography	138:151	arg1	spectrometry					165:176	Liquid chromatography tandem mass spectrometry	131:176	Liquid chromatography tandem mass spectrometry	131:176	Liquid chromatography tandem mass spectrometry has been a widely used technique for quantifying oligonucleotides in biological samples.
32841769	1	41	theme	chromatography	138:151	arg1	technique					201:209	a widely used technique	187:209	a widely used technique for quantifying oligonucleotides in biological samples	187:264	Liquid chromatography tandem mass spectrometry has been a widely used technique for quantifying oligonucleotides in biological samples.
32841769	11	42	theme	0.25-1000 nM	1398:1409	arg1	range					1389:1393	a quantitative range	1374:1393	a quantitative range of 0.25-1000 nM	1374:1409	The developed assay provided a quantitative range of 0.25-1000 nM, with accuracy and precision were < 7.45% and < 12.20%, respectively.
32841769	9	43	theme	MS	1247:1248	arg1	parameters					1250:1259	MS parameters	1247:1259	MS parameters	1247:1259	It was found that the central composite design suite was proved to be highly practical for optimizing MS parameters.
32841769	1	44	theme	tandem	153:158	arg1	spectrometry					165:176	Liquid chromatography tandem mass spectrometry	131:176	Liquid chromatography tandem mass spectrometry	131:176	Liquid chromatography tandem mass spectrometry has been a widely used technique for quantifying oligonucleotides in biological samples.
32841769	1	44	theme	tandem	153:158	arg1	technique					201:209	a widely used technique	187:209	a widely used technique for quantifying oligonucleotides in biological samples	187:264	Liquid chromatography tandem mass spectrometry has been a widely used technique for quantifying oligonucleotides in biological samples.
32841769	12	45	theme	significant	1536:1546	arg1	effect					1548:1553	no significant effect	1533:1553	no significant effect	1533:1553	Matrix effect and carryover were also evaluated and no significant effect was observed.
32841769	5	46	theme	solid-phase	735:745	arg1	SPE					759:761	SPE	759:761	SPE	759:761	These included liquid-liquid extraction (LLE), solid-phase extraction (SPE), protein precipitation (PPT) and LLE combined with SPE.
32841769	5	46	theme	solid-phase	735:745	arg1	extraction					747:756	solid-phase extraction	735:756	solid-phase extraction (SPE)	735:762	These included liquid-liquid extraction (LLE), solid-phase extraction (SPE), protein precipitation (PPT) and LLE combined with SPE.
32841769	2	47	theme	efficient	295:303	arg1	approach					320:327	simple and efficient sample cleanup approach	284:327	simple and efficient sample cleanup approach	284:327	However, lack of simple and efficient sample cleanup approach remains a challenge.
32841769	7	48	theme	ethanol	985:991	arg1	conditions					1063:1072	the optimal drying conditions	1044:1072	the optimal drying conditions	1044:1072	Followed by the extraction, ethanol precipitation (-80 °C, 5 min) was determined to be the optimal drying conditions.
32841769	7	48	theme	ethanol	985:991	arg1	precipitation					993:1005	ethanol precipitation	985:1005	ethanol precipitation (-80 °C, 5 min)	985:1021	Followed by the extraction, ethanol precipitation (-80 °C, 5 min) was determined to be the optimal drying conditions.
32841769	1	49	theme	mass	160:163	arg1	spectrometry					165:176	Liquid chromatography tandem mass spectrometry	131:176	Liquid chromatography tandem mass spectrometry	131:176	Liquid chromatography tandem mass spectrometry has been a widely used technique for quantifying oligonucleotides in biological samples.
32841769	1	49	theme	mass	160:163	arg1	technique					201:209	a widely used technique	187:209	a widely used technique for quantifying oligonucleotides in biological samples	187:264	Liquid chromatography tandem mass spectrometry has been a widely used technique for quantifying oligonucleotides in biological samples.
32841769	11	50	theme	<	1457:1457	arg1	%					1464:1464	< 12.20%	1457:1464	< 12.20%	1457:1464	The developed assay provided a quantitative range of 0.25-1000 nM, with accuracy and precision were < 7.45% and < 12.20%, respectively.
32841769	8	51	theme	optimal	1125:1131	arg1	conditions					1133:1142	optimal conditions	1125:1142	optimal conditions	1125:1142	Also, mass spectrometric parameters were tuned to optimal conditions.
32841769	10	52	theme	optimized	1286:1294	arg1	workflow					1315:1322	the thoroughly optimized sample preparation workflow	1271:1322	the thoroughly optimized sample preparation workflow	1271:1322	Finally, the thoroughly optimized sample preparation workflow was fully validated.
32841769	7	53	dep	precipitation	993:1005	arg1	5 min					1016:1020	5 min	1016:1020	5 min	1016:1020	Followed by the extraction, ethanol precipitation (-80 °C, 5 min) was determined to be the optimal drying conditions.
32841769	7	53	dep	precipitation	993:1005	arg1	-80 °C					1008:1013	-80 °C	1008:1013	-80 °C	1008:1013	Followed by the extraction, ethanol precipitation (-80 °C, 5 min) was determined to be the optimal drying conditions.
32841769	0	54	theme	liquid	83:88	arg1	spectrometry					117:128	liquid chromatography tandem mass spectrometry	83:128	liquid chromatography tandem mass spectrometry	83:128	Optimizing sample preparation workflow for bioanalysis of oligonucleotides through liquid chromatography tandem mass spectrometry.
32841769	2	55	theme	simple	284:289	arg1	approach					320:327	simple and efficient sample cleanup approach	284:327	simple and efficient sample cleanup approach	284:327	However, lack of simple and efficient sample cleanup approach remains a challenge.
32841769	4	56	theme	preparation	664:674	arg1	procedures					676:685	the sample preparation procedures	653:685	the sample preparation procedures	653:685	In this study, we have employed a model formed with rat plasma containing a 16 mer oligonucleotide standard in order to comprehensively optimize the sample preparation procedures.
32841769	6	57	with	LLE	820:822	arg1	phenol					829:834	phenol	829:834	phenol	829:834	LLE with phenol: dichloromethane (2:1, v:v) was found to be the most efficient sample cleanup procedure with low cost and less toxicity.
32841769	6	58	theme	cleanup	906:912	arg1	procedure					914:922	the most efficient sample cleanup procedure	880:922	the most efficient sample cleanup procedure with low cost and less toxicity	880:954	LLE with phenol: dichloromethane (2:1, v:v) was found to be the most efficient sample cleanup procedure with low cost and less toxicity.
32841769	6	58	theme	cleanup	906:912	arg1	dichloromethane					837:851	dichloromethane	837:851	dichloromethane (2:1, v:v)	837:862	LLE with phenol: dichloromethane (2:1, v:v) was found to be the most efficient sample cleanup procedure with low cost and less toxicity.
32841769	3	59	theme	pretreatment	414:425	arg1	process					427:433	the sample pretreatment process	403:433	the sample pretreatment process for developing optimal sample preparation workflow for oligonucleotides	403:505	Our study aimed to evaluate the major factors during the sample pretreatment process for developing optimal sample preparation workflow for oligonucleotides.
32841769	6	60	theme	low	929:931	arg1	cost					933:936	low cost	929:936	low cost	929:936	LLE with phenol: dichloromethane (2:1, v:v) was found to be the most efficient sample cleanup procedure with low cost and less toxicity.
32841769	4	61	theme	sample	657:662	arg1	procedures					676:685	the sample preparation procedures	653:685	the sample preparation procedures	653:685	In this study, we have employed a model formed with rat plasma containing a 16 mer oligonucleotide standard in order to comprehensively optimize the sample preparation procedures.
32522944	0	0	theme	Complex	94:100	arg1	Coacervation					102:113	Complex Coacervation	94:113	Complex Coacervation	94:113	Characterization and Oxidative Stability of Cold-pressed Sesame Oil Microcapsules Prepared by Complex Coacervation.
32522944	1	1	theme	high	166:169	arg1	value					183:187	high nutritional value	166:187	high nutritional value	166:187	Although cold-pressed sesame oil (CPSO) possesses high nutritional value, its application in the food industry is limited due to its poor oxidative stability.
32522944	6	2	theme	coacervation	981:992	arg1	process					962:968	the process	958:968	the process of complex coacervation	958:992	The FTIR study confirmed that the process of complex coacervation was formed between gelatin and gum Arabic by electrostatic interactions.
32522944	8	3	from	content	1172:1178	arg1	CPSO					1219:1222	CPSO	1219:1222	CPSO	1219:1222	The fatty acid composition, the content of sesamin, sesamolin and vitamin E in CPSO were determined before and after microencapsulation.
32522944	7	4	theme	good	1118:1121	arg1	resistance					1128:1137	good heat resistance	1118:1137	good heat resistance	1118:1137	The TGA study suggested that the microcapsules had good heat resistance.
32522944	1	5	theme	nutritional	171:181	arg1	value					183:187	high nutritional value	166:187	high nutritional value	166:187	Although cold-pressed sesame oil (CPSO) possesses high nutritional value, its application in the food industry is limited due to its poor oxidative stability.
32522944	1	6	theme	poor	249:252	arg1	stability					264:272	its poor oxidative stability	245:272	its poor oxidative stability	245:272	Although cold-pressed sesame oil (CPSO) possesses high nutritional value, its application in the food industry is limited due to its poor oxidative stability.
32522944	8	7	theme	acid	1150:1153	arg1	composition					1155:1165	The fatty acid composition	1140:1165	The fatty acid composition	1140:1165	The fatty acid composition, the content of sesamin, sesamolin and vitamin E in CPSO were determined before and after microencapsulation.
32522944	2	8	with	technology	381:390	arg1	gum					409:411	gum	409:411	gum	409:411	The aim of this study was to enhance the oxidative stability of CPSO by complex coacervation microcapsule technology with gelatin and gum Arabic as wall materials.
32522944	2	8	with	technology	381:390	arg1	gelatin					397:403	gelatin	397:403	gelatin	397:403	The aim of this study was to enhance the oxidative stability of CPSO by complex coacervation microcapsule technology with gelatin and gum Arabic as wall materials.
32522944	3	9	theme	laser	529:533	arg1	analyzer					562:569	a laser particle size distribution analyzer	527:569	a laser particle size distribution analyzer	527:569	The characterization of CPSO microcapsules were evaluated by a particle image analyzer, a laser particle size distribution analyzer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
32522944	3	9	theme	laser	529:533	arg1	analyzer					517:524	a particle image analyzer	500:524	a particle image analyzer	500:524	The characterization of CPSO microcapsules were evaluated by a particle image analyzer, a laser particle size distribution analyzer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
32522944	1	10	theme	cold-pressed	125:136	arg1	CPSO					150:153	CPSO	150:153	CPSO	150:153	Although cold-pressed sesame oil (CPSO) possesses high nutritional value, its application in the food industry is limited due to its poor oxidative stability.
32522944	1	10	theme	cold-pressed	125:136	arg1	oil					145:147	cold-pressed sesame oil	125:147	cold-pressed sesame oil (CPSO)	125:154	Although cold-pressed sesame oil (CPSO) possesses high nutritional value, its application in the food industry is limited due to its poor oxidative stability.
32522944	1	11	theme	oxidative	254:262	arg1	stability					264:272	its poor oxidative stability	245:272	its poor oxidative stability	245:272	Although cold-pressed sesame oil (CPSO) possesses high nutritional value, its application in the food industry is limited due to its poor oxidative stability.
32522944	8	12	theme	fatty	1144:1148	arg1	composition					1155:1165	The fatty acid composition	1140:1165	The fatty acid composition	1140:1165	The fatty acid composition, the content of sesamin, sesamolin and vitamin E in CPSO were determined before and after microencapsulation.
32522944	3	13	theme	particle	535:542	arg1	analyzer					562:569	a laser particle size distribution analyzer	527:569	a laser particle size distribution analyzer	527:569	The characterization of CPSO microcapsules were evaluated by a particle image analyzer, a laser particle size distribution analyzer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
32522944	3	13	theme	particle	535:542	arg1	analyzer					517:524	a particle image analyzer	500:524	a particle image analyzer	500:524	The characterization of CPSO microcapsules were evaluated by a particle image analyzer, a laser particle size distribution analyzer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
32522944	2	14	theme	CPSO	339:342	arg1	stability					326:334	the oxidative stability	312:334	the oxidative stability of CPSO	312:342	The aim of this study was to enhance the oxidative stability of CPSO by complex coacervation microcapsule technology with gelatin and gum Arabic as wall materials.
32522944	5	15	theme	spherical	904:912	arg1	microcapsule					914:925	a multinuclear spherical microcapsule	889:925	a multinuclear spherical microcapsule	889:925	The average particle size of the microcapsules was approximately 117.1 μm and many oil droplets were encapsulated by complex coacervation to form a multinuclear spherical microcapsule.
32522944	3	16	theme	CPSO	463:466	arg1	microcapsules					468:480	CPSO microcapsules	463:480	CPSO microcapsules	463:480	The characterization of CPSO microcapsules were evaluated by a particle image analyzer, a laser particle size distribution analyzer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
32522944	9	17	theme	microencapsulation	1296:1313	arg1	process					1315:1321	the microencapsulation process	1292:1321	the microencapsulation process	1292:1321	It showed that the microencapsulation process had almost no effect on the fatty acid composition, sesamin and sesamolin, only Vitamin E was slightly lost during the microencapsulation process.
32522944	5	18	theme	oil	826:828	arg1	droplets					830:837	many oil droplets	821:837	many oil droplets	821:837	The average particle size of the microcapsules was approximately 117.1 μm and many oil droplets were encapsulated by complex coacervation to form a multinuclear spherical microcapsule.
32522944	9	19	theme	Vitamin	1403:1409	arg1	E					1411:1411	Vitamin E	1403:1411	Vitamin E	1403:1411	It showed that the microencapsulation process had almost no effect on the fatty acid composition, sesamin and sesamolin, only Vitamin E was slightly lost during the microencapsulation process.
32522944	1	20	contain	possesses	156:164	arg2	value					183:187	high nutritional value	166:187	high nutritional value	166:187	Although cold-pressed sesame oil (CPSO) possesses high nutritional value, its application in the food industry is limited due to its poor oxidative stability.
32522944	1	20	contain	possesses	156:164	arg1	CPSO					150:153	CPSO	150:153	CPSO	150:153	Although cold-pressed sesame oil (CPSO) possesses high nutritional value, its application in the food industry is limited due to its poor oxidative stability.
32522944	1	20	contain	possesses	156:164	arg1	oil					145:147	cold-pressed sesame oil	125:147	cold-pressed sesame oil (CPSO)	125:154	Although cold-pressed sesame oil (CPSO) possesses high nutritional value, its application in the food industry is limited due to its poor oxidative stability.
32522944	5	21	theme	average	747:753	arg1	size					764:767	The average particle size	743:767	The average particle size of the microcapsules	743:788	The average particle size of the microcapsules was approximately 117.1 μm and many oil droplets were encapsulated by complex coacervation to form a multinuclear spherical microcapsule.
32522944	5	21	theme	average	747:753	arg1	μm					814:815	approximately 117.1 μm	794:815	approximately 117.1 μm	794:815	The average particle size of the microcapsules was approximately 117.1 μm and many oil droplets were encapsulated by complex coacervation to form a multinuclear spherical microcapsule.
32522944	9	22	contain	had	1323:1325	arg2	effect					1337:1342	almost no effect	1327:1342	almost no effect	1327:1342	It showed that the microencapsulation process had almost no effect on the fatty acid composition, sesamin and sesamolin, only Vitamin E was slightly lost during the microencapsulation process.
32522944	9	22	contain	had	1323:1325	arg1	process					1315:1321	the microencapsulation process	1292:1321	the microencapsulation process	1292:1321	It showed that the microencapsulation process had almost no effect on the fatty acid composition, sesamin and sesamolin, only Vitamin E was slightly lost during the microencapsulation process.
32522944	7	23	theme	TGA	1071:1073	arg1	study					1075:1079	The TGA study	1067:1079	The TGA study	1067:1079	The TGA study suggested that the microcapsules had good heat resistance.
32522944	2	24	theme	microcapsule	368:379	arg1	technology					381:390	complex coacervation microcapsule technology	347:390	complex coacervation microcapsule technology with gelatin and gum Arabic as wall materials	347:436	The aim of this study was to enhance the oxidative stability of CPSO by complex coacervation microcapsule technology with gelatin and gum Arabic as wall materials.
32522944	5	25	theme	microcapsules	776:788	arg1	size					764:767	The average particle size	743:767	The average particle size of the microcapsules	743:788	The average particle size of the microcapsules was approximately 117.1 μm and many oil droplets were encapsulated by complex coacervation to form a multinuclear spherical microcapsule.
32522944	5	25	theme	microcapsules	776:788	arg1	μm					814:815	approximately 117.1 μm	794:815	approximately 117.1 μm	794:815	The average particle size of the microcapsules was approximately 117.1 μm and many oil droplets were encapsulated by complex coacervation to form a multinuclear spherical microcapsule.
32522944	3	26	theme	distribution	549:560	arg1	analyzer					562:569	a laser particle size distribution analyzer	527:569	a laser particle size distribution analyzer	527:569	The characterization of CPSO microcapsules were evaluated by a particle image analyzer, a laser particle size distribution analyzer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
32522944	3	26	theme	distribution	549:560	arg1	analyzer					517:524	a particle image analyzer	500:524	a particle image analyzer	500:524	The characterization of CPSO microcapsules were evaluated by a particle image analyzer, a laser particle size distribution analyzer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
32522944	2	27	theme	coacervation	355:366	arg1	technology					381:390	complex coacervation microcapsule technology	347:390	complex coacervation microcapsule technology with gelatin and gum Arabic as wall materials	347:436	The aim of this study was to enhance the oxidative stability of CPSO by complex coacervation microcapsule technology with gelatin and gum Arabic as wall materials.
32522944	2	28	theme	wall	423:426	arg1	materials					428:436	wall materials	423:436	wall materials	423:436	The aim of this study was to enhance the oxidative stability of CPSO by complex coacervation microcapsule technology with gelatin and gum Arabic as wall materials.
32522944	10	29	theme	storage	1486:1492	arg1	test					1494:1497	The accelerated storage test	1470:1497	The accelerated storage test	1470:1497	The accelerated storage test showed that microencapsulation significantly increased the oxidative stability of CPSO.
32522944	2	30	theme	study	291:295	arg1	aim					279:281	The aim	275:281	The aim of this study	275:295	The aim of this study was to enhance the oxidative stability of CPSO by complex coacervation microcapsule technology with gelatin and gum Arabic as wall materials.
32522944	0	31	theme	Oxidative	21:29	arg1	Stability					31:39	Oxidative Stability	21:39	Oxidative Stability	21:39	Characterization and Oxidative Stability of Cold-pressed Sesame Oil Microcapsules Prepared by Complex Coacervation.
32522944	10	32	theme	accelerated	1474:1484	arg1	test					1494:1497	The accelerated storage test	1470:1497	The accelerated storage test	1470:1497	The accelerated storage test showed that microencapsulation significantly increased the oxidative stability of CPSO.
32522944	0	33	dep	Microcapsules	68:80	arg1	Prepared					82:89	Prepared	82:89	Microcapsules Prepared by Complex Coacervation	68:113	Characterization and Oxidative Stability of Cold-pressed Sesame Oil Microcapsules Prepared by Complex Coacervation.
32522944	3	34	theme	image	511:515	arg1	microscopy					590:599	scanning electron microscopy	572:599	scanning electron microscopy (SEM)	572:605	The characterization of CPSO microcapsules were evaluated by a particle image analyzer, a laser particle size distribution analyzer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
32522944	3	34	theme	image	511:515	arg1	Fourier					608:614	Fourier	608:614	Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA)	608:690	The characterization of CPSO microcapsules were evaluated by a particle image analyzer, a laser particle size distribution analyzer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
32522944	3	34	theme	image	511:515	arg1	analyzer					562:569	a laser particle size distribution analyzer	527:569	a laser particle size distribution analyzer	527:569	The characterization of CPSO microcapsules were evaluated by a particle image analyzer, a laser particle size distribution analyzer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
32522944	3	34	theme	image	511:515	arg1	analyzer					517:524	a particle image analyzer	500:524	a particle image analyzer	500:524	The characterization of CPSO microcapsules were evaluated by a particle image analyzer, a laser particle size distribution analyzer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
32522944	0	35	theme	Cold-pressed	44:55	arg1	Oil					64:66	Cold-pressed Sesame Oil	44:66	Cold-pressed Sesame Oil	44:66	Characterization and Oxidative Stability of Cold-pressed Sesame Oil Microcapsules Prepared by Complex Coacervation.
32522944	8	36	from	sesamolin	1192:1200	arg1	CPSO					1219:1222	CPSO	1219:1222	CPSO	1219:1222	The fatty acid composition, the content of sesamin, sesamolin and vitamin E in CPSO were determined before and after microencapsulation.
32522944	9	37	theme	microencapsulation	1442:1459	arg1	process					1461:1467	the microencapsulation process	1438:1467	the microencapsulation process	1438:1467	It showed that the microencapsulation process had almost no effect on the fatty acid composition, sesamin and sesamolin, only Vitamin E was slightly lost during the microencapsulation process.
32522944	6	38	theme	electrostatic	1039:1051	arg1	interactions					1053:1064	electrostatic interactions	1039:1064	electrostatic interactions	1039:1064	The FTIR study confirmed that the process of complex coacervation was formed between gelatin and gum Arabic by electrostatic interactions.
32522944	1	39	theme	food	213:216	arg1	industry					218:225	the food industry	209:225	the food industry	209:225	Although cold-pressed sesame oil (CPSO) possesses high nutritional value, its application in the food industry is limited due to its poor oxidative stability.
32522944	5	40	theme	particle	755:762	arg1	size					764:767	The average particle size	743:767	The average particle size of the microcapsules	743:788	The average particle size of the microcapsules was approximately 117.1 μm and many oil droplets were encapsulated by complex coacervation to form a multinuclear spherical microcapsule.
32522944	5	40	theme	particle	755:762	arg1	μm					814:815	approximately 117.1 μm	794:815	approximately 117.1 μm	794:815	The average particle size of the microcapsules was approximately 117.1 μm and many oil droplets were encapsulated by complex coacervation to form a multinuclear spherical microcapsule.
32522944	6	41	theme	FTIR	932:935	arg1	study					937:941	The FTIR study	928:941	The FTIR study	928:941	The FTIR study confirmed that the process of complex coacervation was formed between gelatin and gum Arabic by electrostatic interactions.
32522944	6	42	theme	complex	973:979	arg1	coacervation					981:992	complex coacervation	973:992	complex coacervation	973:992	The FTIR study confirmed that the process of complex coacervation was formed between gelatin and gum Arabic by electrostatic interactions.
32522944	8	43	theme	sesamin	1183:1189	arg1	sesamolin					1192:1200	sesamolin	1192:1200	sesamolin	1192:1200	The fatty acid composition, the content of sesamin, sesamolin and vitamin E in CPSO were determined before and after microencapsulation.
32522944	8	43	theme	sesamin	1183:1189	arg1	E					1214:1214	vitamin E	1206:1214	vitamin E	1206:1214	The fatty acid composition, the content of sesamin, sesamolin and vitamin E in CPSO were determined before and after microencapsulation.
32522944	8	43	theme	sesamin	1183:1189	arg1	composition					1155:1165	The fatty acid composition	1140:1165	The fatty acid composition	1140:1165	The fatty acid composition, the content of sesamin, sesamolin and vitamin E in CPSO were determined before and after microencapsulation.
32522944	8	43	theme	sesamin	1183:1189	arg1	content					1172:1178	the content	1168:1178	the content of sesamin	1168:1189	The fatty acid composition, the content of sesamin, sesamolin and vitamin E in CPSO were determined before and after microencapsulation.
32522944	3	44	dep	Fourier	608:614	arg1	transform					616:624	transform	616:624	transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA)	616:690	The characterization of CPSO microcapsules were evaluated by a particle image analyzer, a laser particle size distribution analyzer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
32522944	0	45	theme	Oil	64:66	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and Oxidative Stability of Cold-pressed Sesame Oil Microcapsules Prepared by Complex Coacervation.
32522944	0	45	theme	Oil	64:66	arg1	Stability					31:39	Oxidative Stability	21:39	Oxidative Stability	21:39	Characterization and Oxidative Stability of Cold-pressed Sesame Oil Microcapsules Prepared by Complex Coacervation.
32522944	3	46	theme	scanning	572:579	arg1	microscopy					590:599	scanning electron microscopy	572:599	scanning electron microscopy (SEM)	572:605	The characterization of CPSO microcapsules were evaluated by a particle image analyzer, a laser particle size distribution analyzer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
32522944	3	46	theme	scanning	572:579	arg1	SEM					602:604	SEM	602:604	SEM	602:604	The characterization of CPSO microcapsules were evaluated by a particle image analyzer, a laser particle size distribution analyzer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
32522944	3	46	theme	scanning	572:579	arg1	analyzer					517:524	a particle image analyzer	500:524	a particle image analyzer	500:524	The characterization of CPSO microcapsules were evaluated by a particle image analyzer, a laser particle size distribution analyzer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
32522944	6	47	dep	gelatin	1013:1019	arg1	Arabic					1029:1034	Arabic	1029:1034	Arabic	1029:1034	The FTIR study confirmed that the process of complex coacervation was formed between gelatin and gum Arabic by electrostatic interactions.
32522944	1	48	theme	sesame	138:143	arg1	CPSO					150:153	CPSO	150:153	CPSO	150:153	Although cold-pressed sesame oil (CPSO) possesses high nutritional value, its application in the food industry is limited due to its poor oxidative stability.
32522944	1	48	theme	sesame	138:143	arg1	oil					145:147	cold-pressed sesame oil	125:147	cold-pressed sesame oil (CPSO)	125:154	Although cold-pressed sesame oil (CPSO) possesses high nutritional value, its application in the food industry is limited due to its poor oxidative stability.
32522944	0	49	theme	Sesame	57:62	arg1	Oil					64:66	Cold-pressed Sesame Oil	44:66	Cold-pressed Sesame Oil	44:66	Characterization and Oxidative Stability of Cold-pressed Sesame Oil Microcapsules Prepared by Complex Coacervation.
32522944	9	50	theme	fatty	1351:1355	arg1	composition					1362:1372	the fatty acid composition	1347:1372	the fatty acid composition	1347:1372	It showed that the microencapsulation process had almost no effect on the fatty acid composition, sesamin and sesamolin, only Vitamin E was slightly lost during the microencapsulation process.
32522944	3	51	theme	electron	581:588	arg1	microscopy					590:599	scanning electron microscopy	572:599	scanning electron microscopy (SEM)	572:605	The characterization of CPSO microcapsules were evaluated by a particle image analyzer, a laser particle size distribution analyzer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
32522944	3	51	theme	electron	581:588	arg1	SEM					602:604	SEM	602:604	SEM	602:604	The characterization of CPSO microcapsules were evaluated by a particle image analyzer, a laser particle size distribution analyzer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
32522944	3	51	theme	electron	581:588	arg1	analyzer					517:524	a particle image analyzer	500:524	a particle image analyzer	500:524	The characterization of CPSO microcapsules were evaluated by a particle image analyzer, a laser particle size distribution analyzer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
32522944	3	52	theme	particle	502:509	arg1	microscopy					590:599	scanning electron microscopy	572:599	scanning electron microscopy (SEM)	572:605	The characterization of CPSO microcapsules were evaluated by a particle image analyzer, a laser particle size distribution analyzer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
32522944	3	52	theme	particle	502:509	arg1	Fourier					608:614	Fourier	608:614	Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA)	608:690	The characterization of CPSO microcapsules were evaluated by a particle image analyzer, a laser particle size distribution analyzer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
32522944	3	52	theme	particle	502:509	arg1	analyzer					562:569	a laser particle size distribution analyzer	527:569	a laser particle size distribution analyzer	527:569	The characterization of CPSO microcapsules were evaluated by a particle image analyzer, a laser particle size distribution analyzer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
32522944	3	52	theme	particle	502:509	arg1	analyzer					517:524	a particle image analyzer	500:524	a particle image analyzer	500:524	The characterization of CPSO microcapsules were evaluated by a particle image analyzer, a laser particle size distribution analyzer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
32522944	2	53	theme	oxidative	316:324	arg1	stability					326:334	the oxidative stability	312:334	the oxidative stability of CPSO	312:342	The aim of this study was to enhance the oxidative stability of CPSO by complex coacervation microcapsule technology with gelatin and gum Arabic as wall materials.
32522944	9	54	dep	had	1323:1325	arg1	lost					1426:1429	lost	1426:1429	was slightly lost during the microencapsulation process	1413:1467	It showed that the microencapsulation process had almost no effect on the fatty acid composition, sesamin and sesamolin, only Vitamin E was slightly lost during the microencapsulation process.
32522944	9	55	theme	acid	1357:1360	arg1	composition					1362:1372	the fatty acid composition	1347:1372	the fatty acid composition	1347:1372	It showed that the microencapsulation process had almost no effect on the fatty acid composition, sesamin and sesamolin, only Vitamin E was slightly lost during the microencapsulation process.
32522944	3	56	theme	thermogravimetric	659:675	arg1	TGA					687:689	TGA	687:689	TGA	687:689	The characterization of CPSO microcapsules were evaluated by a particle image analyzer, a laser particle size distribution analyzer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
32522944	3	56	theme	thermogravimetric	659:675	arg1	analysis					677:684	thermogravimetric analysis	659:684	thermogravimetric analysis (TGA)	659:690	The characterization of CPSO microcapsules were evaluated by a particle image analyzer, a laser particle size distribution analyzer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
32522944	8	57	from	E	1214:1214	arg1	CPSO					1219:1222	CPSO	1219:1222	CPSO	1219:1222	The fatty acid composition, the content of sesamin, sesamolin and vitamin E in CPSO were determined before and after microencapsulation.
32522944	3	58	theme	microcapsules	468:480	arg1	characterization					443:458	The characterization	439:458	The characterization of CPSO microcapsules	439:480	The characterization of CPSO microcapsules were evaluated by a particle image analyzer, a laser particle size distribution analyzer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
32522944	3	59	theme	size	544:547	arg1	analyzer					562:569	a laser particle size distribution analyzer	527:569	a laser particle size distribution analyzer	527:569	The characterization of CPSO microcapsules were evaluated by a particle image analyzer, a laser particle size distribution analyzer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
32522944	3	59	theme	size	544:547	arg1	analyzer					517:524	a particle image analyzer	500:524	a particle image analyzer	500:524	The characterization of CPSO microcapsules were evaluated by a particle image analyzer, a laser particle size distribution analyzer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
32522944	3	60	dep	transform	616:624	arg1	infrared					626:633	infrared	626:633	transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA)	616:690	The characterization of CPSO microcapsules were evaluated by a particle image analyzer, a laser particle size distribution analyzer, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA).
32522944	7	61	contain	had	1114:1116	arg1	microcapsules					1100:1112	the microcapsules	1096:1112	the microcapsules	1096:1112	The TGA study suggested that the microcapsules had good heat resistance.
32522944	7	61	contain	had	1114:1116	arg2	resistance					1128:1137	good heat resistance	1118:1137	good heat resistance	1118:1137	The TGA study suggested that the microcapsules had good heat resistance.
32522944	8	62	from	composition	1155:1165	arg1	CPSO					1219:1222	CPSO	1219:1222	CPSO	1219:1222	The fatty acid composition, the content of sesamin, sesamolin and vitamin E in CPSO were determined before and after microencapsulation.
32522944	4	63	theme	encapsulation	697:709	arg1	EE					723:724	EE	723:724	EE	723:724	The encapsulation efficiency (EE) reached 90.25%.
32522944	4	63	theme	encapsulation	697:709	arg1	efficiency					711:720	The encapsulation efficiency	693:720	The encapsulation efficiency (EE)	693:725	The encapsulation efficiency (EE) reached 90.25%.
32522944	8	64	theme	vitamin	1206:1212	arg1	E					1214:1214	vitamin E	1206:1214	vitamin E	1206:1214	The fatty acid composition, the content of sesamin, sesamolin and vitamin E in CPSO were determined before and after microencapsulation.
32522944	5	65	theme	multinuclear	891:902	arg1	microcapsule					914:925	a multinuclear spherical microcapsule	889:925	a multinuclear spherical microcapsule	889:925	The average particle size of the microcapsules was approximately 117.1 μm and many oil droplets were encapsulated by complex coacervation to form a multinuclear spherical microcapsule.
32522944	10	66	theme	oxidative	1558:1566	arg1	stability					1568:1576	the oxidative stability	1554:1576	the oxidative stability of CPSO	1554:1584	The accelerated storage test showed that microencapsulation significantly increased the oxidative stability of CPSO.
32522944	5	67	theme	many	821:824	arg1	droplets					830:837	many oil droplets	821:837	many oil droplets	821:837	The average particle size of the microcapsules was approximately 117.1 μm and many oil droplets were encapsulated by complex coacervation to form a multinuclear spherical microcapsule.
32522944	1	68	from	application	194:204	arg1	industry					218:225	the food industry	209:225	the food industry	209:225	Although cold-pressed sesame oil (CPSO) possesses high nutritional value, its application in the food industry is limited due to its poor oxidative stability.
32522944	2	69	theme	complex	347:353	arg1	technology					381:390	complex coacervation microcapsule technology	347:390	complex coacervation microcapsule technology with gelatin and gum Arabic as wall materials	347:436	The aim of this study was to enhance the oxidative stability of CPSO by complex coacervation microcapsule technology with gelatin and gum Arabic as wall materials.
32522944	7	70	theme	heat	1123:1126	arg1	resistance					1128:1137	good heat resistance	1118:1137	good heat resistance	1118:1137	The TGA study suggested that the microcapsules had good heat resistance.
32522944	5	71	theme	complex	860:866	arg1	coacervation					868:879	complex coacervation	860:879	complex coacervation	860:879	The average particle size of the microcapsules was approximately 117.1 μm and many oil droplets were encapsulated by complex coacervation to form a multinuclear spherical microcapsule.
32522944	10	72	theme	CPSO	1581:1584	arg1	stability					1568:1576	the oxidative stability	1554:1576	the oxidative stability of CPSO	1554:1584	The accelerated storage test showed that microencapsulation significantly increased the oxidative stability of CPSO.
33036488	0	0	theme	Osteoblast/Osteoclast	89:109	arg1	Activity					111:118	the Osteoblast/Osteoclast Activity	85:118	the Osteoblast/Osteoclast Activity Ratio-A Co-Culture Study	85:143	Hemocyanin Modification of Chitosan Scaffolds with Calcium Phosphate Phases Increase the Osteoblast/Osteoclast Activity Ratio-A Co-Culture Study.
33036488	7	1	theme	increased	1284:1292	arg1	proliferation					1294:1306	increased proliferation	1284:1306	increased proliferation of hBMSCs	1284:1316	Dip-coating with hemocyanin led to increased proliferation of hBMSCs and equivalent osteoclast maturation in mono-culture, while in co-culture, both an inhibitory effect of mineral incorporation on osteoblastogenesis and stimulatory effects of hemocyanin were observed.
33036488	2	2	theme	chitosan	381:388	arg1	scaffolds					390:398	porous chitosan scaffolds	374:398	porous chitosan scaffolds	374:398	The bone biocompatibility of porous chitosan scaffolds was modified in the present study by the addition of calcium phosphates or hemocyanin.
33036488	5	3	theme	stromal	816:822	arg1	hBMSCs					831:836	hBMSCs	831:836	hBMSCs	831:836	The cytocompatibility was assessed in a mono-culture of human bone marrow stromal cells (hBMSCs) and their differentiation to osteoblasts; in a mono-culture of human monocytes (hMs) and their maturation to osteoclasts; and in a co-culture of hBMSC/osteoblasts-hM/osteoclasts.
33036488	5	3	theme	stromal	816:822	arg1	cells					824:828	stromal cells	816:828	human bone marrow stromal cells (hBMSCs)	798:837	The cytocompatibility was assessed in a mono-culture of human bone marrow stromal cells (hBMSCs) and their differentiation to osteoblasts; in a mono-culture of human monocytes (hMs) and their maturation to osteoclasts; and in a co-culture of hBMSC/osteoblasts-hM/osteoclasts.
33036488	2	4	theme	present	420:426	arg1	study					428:432	the present study	416:432	the present study	416:432	The bone biocompatibility of porous chitosan scaffolds was modified in the present study by the addition of calcium phosphates or hemocyanin.
33036488	1	5	dep	activity	303:310	arg1	the					299:301	the	299:301	the	299:301	The ongoing research on biomaterials that support bone regeneration led to the quest for materials or material modifications that can actively influence the activity or balance of bone tissue cells.
33036488	9	6	theme	osteoblasts/osteoclasts	1736:1758	arg1	ratio					1769:1773	the osteoblasts/osteoclasts activity ratio	1732:1773	the osteoblasts/osteoclasts activity ratio	1732:1773	These influences on the osteoblasts/osteoclasts activity ratio may support scaffold-driven bone healing in the future.
33036488	7	7	from	maturation	1344:1353	arg1	mono-culture					1358:1369	mono-culture	1358:1369	mono-culture	1358:1369	Dip-coating with hemocyanin led to increased proliferation of hBMSCs and equivalent osteoclast maturation in mono-culture, while in co-culture, both an inhibitory effect of mineral incorporation on osteoblastogenesis and stimulatory effects of hemocyanin were observed.
33036488	2	8	theme	porous	374:379	arg1	scaffolds					390:398	porous chitosan scaffolds	374:398	porous chitosan scaffolds	374:398	The bone biocompatibility of porous chitosan scaffolds was modified in the present study by the addition of calcium phosphates or hemocyanin.
33036488	2	9	theme	bone	349:352	arg1	biocompatibility					354:369	The bone biocompatibility	345:369	The bone biocompatibility of porous chitosan scaffolds	345:398	The bone biocompatibility of porous chitosan scaffolds was modified in the present study by the addition of calcium phosphates or hemocyanin.
33036488	0	10	theme	Ratio-A	120:126	arg1	Study					139:143	Ratio-A Co-Culture Study	120:143	the Osteoblast/Osteoclast Activity Ratio-A Co-Culture Study	85:143	Hemocyanin Modification of Chitosan Scaffolds with Calcium Phosphate Phases Increase the Osteoblast/Osteoclast Activity Ratio-A Co-Culture Study.
33036488	3	11	theme	chitosan-mineral	593:608	arg1	composite					616:624	a biomimetic chitosan-mineral phase composite	580:624	a biomimetic chitosan-mineral phase composite	580:624	The first strategy comprised the incorporation of calcium phosphates into chitosan to create a biomimetic chitosan-mineral phase composite.
33036488	8	12	dep	osteoblast	1605:1614	arg1	development					1631:1641	development	1631:1641	development	1631:1641	It was concluded that highly bioactive scaffolds (containing mineral phases) restrain osteoblast and osteoclast development, while hemocyanin coating significantly supports osteoblastogenesis.
33036488	5	13	theme	bone	804:807	arg1	marrow					809:814	human bone marrow stromal cells (hBMSCs)	798:837	human bone marrow stromal cells (hBMSCs)	798:837	The cytocompatibility was assessed in a mono-culture of human bone marrow stromal cells (hBMSCs) and their differentiation to osteoblasts; in a mono-culture of human monocytes (hMs) and their maturation to osteoclasts; and in a co-culture of hBMSC/osteoblasts-hM/osteoclasts.
33036488	7	14	theme	equivalent	1322:1331	arg1	maturation					1344:1353	equivalent osteoclast maturation	1322:1353	equivalent osteoclast maturation in mono-culture	1322:1369	Dip-coating with hemocyanin led to increased proliferation of hBMSCs and equivalent osteoclast maturation in mono-culture, while in co-culture, both an inhibitory effect of mineral incorporation on osteoblastogenesis and stimulatory effects of hemocyanin were observed.
33036488	2	15	mod	modified	404:411	arg1	biocompatibility					354:369	The bone biocompatibility	345:369	The bone biocompatibility of porous chitosan scaffolds	345:398	The bone biocompatibility of porous chitosan scaffolds was modified in the present study by the addition of calcium phosphates or hemocyanin.
33036488	2	15	mod	modified	404:411	arg3	addition					441:448	the addition	437:448	the addition of calcium phosphates or hemocyanin	437:484	The bone biocompatibility of porous chitosan scaffolds was modified in the present study by the addition of calcium phosphates or hemocyanin.
33036488	4	16	theme	second	631:636	arg1	strategy					638:645	The second strategy	627:645	The second strategy	627:645	The second strategy comprised dip-coating of chitosan scaffolds with hemocyanin extracted from crayfish hemolymph.
33036488	5	17	theme	marrow	809:814	arg1	mono-culture					782:793	a mono-culture	780:793	a mono-culture of human bone marrow stromal cells (hBMSCs)	780:837	The cytocompatibility was assessed in a mono-culture of human bone marrow stromal cells (hBMSCs) and their differentiation to osteoblasts; in a mono-culture of human monocytes (hMs) and their maturation to osteoclasts; and in a co-culture of hBMSC/osteoblasts-hM/osteoclasts.
33036488	7	18	theme	osteoclast	1333:1342	arg1	maturation					1344:1353	equivalent osteoclast maturation	1322:1353	equivalent osteoclast maturation in mono-culture	1322:1369	Dip-coating with hemocyanin led to increased proliferation of hBMSCs and equivalent osteoclast maturation in mono-culture, while in co-culture, both an inhibitory effect of mineral incorporation on osteoblastogenesis and stimulatory effects of hemocyanin were observed.
33036488	4	19	theme	scaffolds	681:689	arg1	dip-coating					657:667	dip-coating	657:667	dip-coating of chitosan scaffolds with hemocyanin extracted from crayfish hemolymph	657:739	The second strategy comprised dip-coating of chitosan scaffolds with hemocyanin extracted from crayfish hemolymph.
33036488	1	20	theme	bone	196:199	arg1	regeneration					201:212	bone regeneration	196:212	bone regeneration	196:212	The ongoing research on biomaterials that support bone regeneration led to the quest for materials or material modifications that can actively influence the activity or balance of bone tissue cells.
33036488	0	21	theme	Co-Culture	128:137	arg1	Study					139:143	Ratio-A Co-Culture Study	120:143	the Osteoblast/Osteoclast Activity Ratio-A Co-Culture Study	85:143	Hemocyanin Modification of Chitosan Scaffolds with Calcium Phosphate Phases Increase the Osteoblast/Osteoclast Activity Ratio-A Co-Culture Study.
33036488	8	22	theme	hemocyanin	1650:1659	arg1	coating					1661:1667	hemocyanin coating	1650:1667	hemocyanin coating	1650:1667	It was concluded that highly bioactive scaffolds (containing mineral phases) restrain osteoblast and osteoclast development, while hemocyanin coating significantly supports osteoblastogenesis.
33036488	6	23	from	increase	1050:1057	arg1	bioactivity					1071:1081	scaffold bioactivity	1062:1081	scaffold bioactivity	1062:1081	Mineral incorporation caused an increase in scaffold bioactivity, as shown by reduced calcium concentration in the cell culture medium, delayed differentiation of hBMSCs, and reduced osteoclastic maturation of hMs in mono-culture.
33036488	0	24	dep	Activity	111:118	arg1	Study					139:143	Ratio-A Co-Culture Study	120:143	the Osteoblast/Osteoclast Activity Ratio-A Co-Culture Study	85:143	Hemocyanin Modification of Chitosan Scaffolds with Calcium Phosphate Phases Increase the Osteoblast/Osteoclast Activity Ratio-A Co-Culture Study.
33036488	6	25	theme	culture	1138:1144	arg1	medium					1146:1151	the cell culture medium	1129:1151	the cell culture medium	1129:1151	Mineral incorporation caused an increase in scaffold bioactivity, as shown by reduced calcium concentration in the cell culture medium, delayed differentiation of hBMSCs, and reduced osteoclastic maturation of hMs in mono-culture.
33036488	6	26	theme	hMs	1228:1230	arg1	maturation					1214:1223	osteoclastic maturation	1201:1223	osteoclastic maturation of hMs in mono-culture	1201:1246	Mineral incorporation caused an increase in scaffold bioactivity, as shown by reduced calcium concentration in the cell culture medium, delayed differentiation of hBMSCs, and reduced osteoclastic maturation of hMs in mono-culture.
33036488	1	27	from	research	158:165	arg1	biomaterials					170:181	biomaterials	170:181	biomaterials that support bone regeneration	170:212	The ongoing research on biomaterials that support bone regeneration led to the quest for materials or material modifications that can actively influence the activity or balance of bone tissue cells.
33036488	3	28	theme	phase	610:614	arg1	composite					616:624	a biomimetic chitosan-mineral phase composite	580:624	a biomimetic chitosan-mineral phase composite	580:624	The first strategy comprised the incorporation of calcium phosphates into chitosan to create a biomimetic chitosan-mineral phase composite.
33036488	7	29	with	Dip-coating	1249:1259	arg1	hemocyanin					1266:1275	hemocyanin	1266:1275	hemocyanin	1266:1275	Dip-coating with hemocyanin led to increased proliferation of hBMSCs and equivalent osteoclast maturation in mono-culture, while in co-culture, both an inhibitory effect of mineral incorporation on osteoblastogenesis and stimulatory effects of hemocyanin were observed.
33036488	6	30	theme	cell	1133:1136	arg1	medium					1146:1151	the cell culture medium	1129:1151	the cell culture medium	1129:1151	Mineral incorporation caused an increase in scaffold bioactivity, as shown by reduced calcium concentration in the cell culture medium, delayed differentiation of hBMSCs, and reduced osteoclastic maturation of hMs in mono-culture.
33036488	0	31	theme	Hemocyanin	0:9	arg1	Modification					11:22	Hemocyanin Modification	0:22	Hemocyanin Modification of Chitosan Scaffolds with Calcium Phosphate Phases	0:74	Hemocyanin Modification of Chitosan Scaffolds with Calcium Phosphate Phases Increase the Osteoblast/Osteoclast Activity Ratio-A Co-Culture Study.
33036488	4	32	theme	crayfish	722:729	arg1	hemolymph					731:739	crayfish hemolymph	722:739	crayfish hemolymph	722:739	The second strategy comprised dip-coating of chitosan scaffolds with hemocyanin extracted from crayfish hemolymph.
33036488	8	33	theme	mineral	1580:1586	arg1	phases					1588:1593	mineral phases	1580:1593	mineral phases	1580:1593	It was concluded that highly bioactive scaffolds (containing mineral phases) restrain osteoblast and osteoclast development, while hemocyanin coating significantly supports osteoblastogenesis.
33036488	5	34	theme	human	798:802	arg1	marrow					809:814	human bone marrow stromal cells (hBMSCs)	798:837	human bone marrow stromal cells (hBMSCs)	798:837	The cytocompatibility was assessed in a mono-culture of human bone marrow stromal cells (hBMSCs) and their differentiation to osteoblasts; in a mono-culture of human monocytes (hMs) and their maturation to osteoclasts; and in a co-culture of hBMSC/osteoblasts-hM/osteoclasts.
33036488	7	35	theme	inhibitory	1401:1410	arg1	effect					1412:1417	an inhibitory effect	1398:1417	an inhibitory effect of mineral incorporation on osteoblastogenesis	1398:1464	Dip-coating with hemocyanin led to increased proliferation of hBMSCs and equivalent osteoclast maturation in mono-culture, while in co-culture, both an inhibitory effect of mineral incorporation on osteoblastogenesis and stimulatory effects of hemocyanin were observed.
33036488	2	36	theme	calcium	453:459	arg1	phosphates					461:470	calcium phosphates	453:470	calcium phosphates	453:470	The bone biocompatibility of porous chitosan scaffolds was modified in the present study by the addition of calcium phosphates or hemocyanin.
33036488	7	37	located	observed	1509:1516	arg2	effect					1412:1417	an inhibitory effect	1398:1417	an inhibitory effect of mineral incorporation on osteoblastogenesis	1398:1464	Dip-coating with hemocyanin led to increased proliferation of hBMSCs and equivalent osteoclast maturation in mono-culture, while in co-culture, both an inhibitory effect of mineral incorporation on osteoblastogenesis and stimulatory effects of hemocyanin were observed.
33036488	7	37	located	observed	1509:1516	arg2	effects					1482:1488	stimulatory effects	1470:1488	stimulatory effects of hemocyanin	1470:1502	Dip-coating with hemocyanin led to increased proliferation of hBMSCs and equivalent osteoclast maturation in mono-culture, while in co-culture, both an inhibitory effect of mineral incorporation on osteoblastogenesis and stimulatory effects of hemocyanin were observed.
33036488	7	37	located	observed	1509:1516	arg1	co-culture					1381:1390	co-culture	1381:1390	co-culture	1381:1390	Dip-coating with hemocyanin led to increased proliferation of hBMSCs and equivalent osteoclast maturation in mono-culture, while in co-culture, both an inhibitory effect of mineral incorporation on osteoblastogenesis and stimulatory effects of hemocyanin were observed.
33036488	6	38	from	concentration	1112:1124	arg1	medium					1146:1151	the cell culture medium	1129:1151	the cell culture medium	1129:1151	Mineral incorporation caused an increase in scaffold bioactivity, as shown by reduced calcium concentration in the cell culture medium, delayed differentiation of hBMSCs, and reduced osteoclastic maturation of hMs in mono-culture.
33036488	3	39	theme	biomimetic	582:591	arg1	composite					616:624	a biomimetic chitosan-mineral phase composite	580:624	a biomimetic chitosan-mineral phase composite	580:624	The first strategy comprised the incorporation of calcium phosphates into chitosan to create a biomimetic chitosan-mineral phase composite.
33036488	2	40	theme	phosphates	461:470	arg1	addition					441:448	the addition	437:448	the addition of calcium phosphates or hemocyanin	437:484	The bone biocompatibility of porous chitosan scaffolds was modified in the present study by the addition of calcium phosphates or hemocyanin.
33036488	8	41	theme	bioactive	1548:1556	arg1	scaffolds					1558:1566	highly bioactive scaffolds	1541:1566	highly bioactive scaffolds (containing mineral phases)	1541:1594	It was concluded that highly bioactive scaffolds (containing mineral phases) restrain osteoblast and osteoclast development, while hemocyanin coating significantly supports osteoblastogenesis.
33036488	3	42	theme	calcium	537:543	arg1	phosphates					545:554	calcium phosphates	537:554	calcium phosphates	537:554	The first strategy comprised the incorporation of calcium phosphates into chitosan to create a biomimetic chitosan-mineral phase composite.
33036488	7	43	from	effects	1482:1488	arg1	osteoblastogenesis					1447:1464	osteoblastogenesis	1447:1464	osteoblastogenesis	1447:1464	Dip-coating with hemocyanin led to increased proliferation of hBMSCs and equivalent osteoclast maturation in mono-culture, while in co-culture, both an inhibitory effect of mineral incorporation on osteoblastogenesis and stimulatory effects of hemocyanin were observed.
33036488	3	44	theme	first	491:495	arg1	strategy					497:504	The first strategy	487:504	The first strategy	487:504	The first strategy comprised the incorporation of calcium phosphates into chitosan to create a biomimetic chitosan-mineral phase composite.
33036488	6	45	theme	Mineral	1018:1024	arg1	incorporation					1026:1038	Mineral incorporation	1018:1038	Mineral incorporation	1018:1038	Mineral incorporation caused an increase in scaffold bioactivity, as shown by reduced calcium concentration in the cell culture medium, delayed differentiation of hBMSCs, and reduced osteoclastic maturation of hMs in mono-culture.
33036488	6	46	theme	calcium	1104:1110	arg1	concentration					1112:1124	reduced calcium concentration	1096:1124	reduced calcium concentration in the cell culture medium	1096:1151	Mineral incorporation caused an increase in scaffold bioactivity, as shown by reduced calcium concentration in the cell culture medium, delayed differentiation of hBMSCs, and reduced osteoclastic maturation of hMs in mono-culture.
33036488	7	47	theme	hemocyanin	1493:1502	arg1	effects					1482:1488	stimulatory effects	1470:1488	stimulatory effects of hemocyanin	1470:1502	Dip-coating with hemocyanin led to increased proliferation of hBMSCs and equivalent osteoclast maturation in mono-culture, while in co-culture, both an inhibitory effect of mineral incorporation on osteoblastogenesis and stimulatory effects of hemocyanin were observed.
33036488	7	47	theme	hemocyanin	1493:1502	arg1	effect					1412:1417	an inhibitory effect	1398:1417	an inhibitory effect of mineral incorporation on osteoblastogenesis	1398:1464	Dip-coating with hemocyanin led to increased proliferation of hBMSCs and equivalent osteoclast maturation in mono-culture, while in co-culture, both an inhibitory effect of mineral incorporation on osteoblastogenesis and stimulatory effects of hemocyanin were observed.
33036488	5	48	theme	human	902:906	arg1	monocytes					908:916	human monocytes	902:916	human monocytes	902:916	The cytocompatibility was assessed in a mono-culture of human bone marrow stromal cells (hBMSCs) and their differentiation to osteoblasts; in a mono-culture of human monocytes (hMs) and their maturation to osteoclasts; and in a co-culture of hBMSC/osteoblasts-hM/osteoclasts.
33036488	0	49	theme	Scaffolds	36:44	arg1	Modification					11:22	Hemocyanin Modification	0:22	Hemocyanin Modification of Chitosan Scaffolds with Calcium Phosphate Phases	0:74	Hemocyanin Modification of Chitosan Scaffolds with Calcium Phosphate Phases Increase the Osteoblast/Osteoclast Activity Ratio-A Co-Culture Study.
33036488	1	50	theme	bone	326:329	arg1	cells					338:342	bone tissue cells	326:342	bone tissue cells	326:342	The ongoing research on biomaterials that support bone regeneration led to the quest for materials or material modifications that can actively influence the activity or balance of bone tissue cells.
33036488	6	51	theme	scaffold	1062:1069	arg1	bioactivity					1071:1081	scaffold bioactivity	1062:1081	scaffold bioactivity	1062:1081	Mineral incorporation caused an increase in scaffold bioactivity, as shown by reduced calcium concentration in the cell culture medium, delayed differentiation of hBMSCs, and reduced osteoclastic maturation of hMs in mono-culture.
33036488	0	52	theme	Chitosan	27:34	arg1	Scaffolds					36:44	Chitosan Scaffolds	27:44	Chitosan Scaffolds	27:44	Hemocyanin Modification of Chitosan Scaffolds with Calcium Phosphate Phases Increase the Osteoblast/Osteoclast Activity Ratio-A Co-Culture Study.
33036488	7	53	theme	stimulatory	1470:1480	arg1	effects					1482:1488	stimulatory effects	1470:1488	stimulatory effects of hemocyanin	1470:1502	Dip-coating with hemocyanin led to increased proliferation of hBMSCs and equivalent osteoclast maturation in mono-culture, while in co-culture, both an inhibitory effect of mineral incorporation on osteoblastogenesis and stimulatory effects of hemocyanin were observed.
33036488	5	54	theme	monocytes	908:916	arg1	hMs					919:921	hMs	919:921	hMs	919:921	The cytocompatibility was assessed in a mono-culture of human bone marrow stromal cells (hBMSCs) and their differentiation to osteoblasts; in a mono-culture of human monocytes (hMs) and their maturation to osteoclasts; and in a co-culture of hBMSC/osteoblasts-hM/osteoclasts.
33036488	5	54	theme	monocytes	908:916	arg1	mono-culture					886:897	a mono-culture	884:897	a mono-culture of human monocytes (hMs)	884:922	The cytocompatibility was assessed in a mono-culture of human bone marrow stromal cells (hBMSCs) and their differentiation to osteoblasts; in a mono-culture of human monocytes (hMs) and their maturation to osteoclasts; and in a co-culture of hBMSC/osteoblasts-hM/osteoclasts.
33036488	5	54	theme	monocytes	908:916	arg1	maturation					934:943	their maturation	928:943	their maturation to osteoclasts	928:958	The cytocompatibility was assessed in a mono-culture of human bone marrow stromal cells (hBMSCs) and their differentiation to osteoblasts; in a mono-culture of human monocytes (hMs) and their maturation to osteoclasts; and in a co-culture of hBMSC/osteoblasts-hM/osteoclasts.
33036488	0	55	theme	Phosphate	59:67	arg1	Phases					69:74	Calcium Phosphate Phases	51:74	Calcium Phosphate Phases	51:74	Hemocyanin Modification of Chitosan Scaffolds with Calcium Phosphate Phases Increase the Osteoblast/Osteoclast Activity Ratio-A Co-Culture Study.
33036488	1	56	theme	tissue	331:336	arg1	cells					338:342	bone tissue cells	326:342	bone tissue cells	326:342	The ongoing research on biomaterials that support bone regeneration led to the quest for materials or material modifications that can actively influence the activity or balance of bone tissue cells.
33036488	5	57	dep	marrow	809:814	arg1	hBMSCs					831:836	hBMSCs	831:836	hBMSCs	831:836	The cytocompatibility was assessed in a mono-culture of human bone marrow stromal cells (hBMSCs) and their differentiation to osteoblasts; in a mono-culture of human monocytes (hMs) and their maturation to osteoclasts; and in a co-culture of hBMSC/osteoblasts-hM/osteoclasts.
33036488	5	57	dep	marrow	809:814	arg1	cells					824:828	stromal cells	816:828	human bone marrow stromal cells (hBMSCs)	798:837	The cytocompatibility was assessed in a mono-culture of human bone marrow stromal cells (hBMSCs) and their differentiation to osteoblasts; in a mono-culture of human monocytes (hMs) and their maturation to osteoclasts; and in a co-culture of hBMSC/osteoblasts-hM/osteoclasts.
33036488	9	58	theme	activity	1760:1767	arg1	ratio					1769:1773	the osteoblasts/osteoclasts activity ratio	1732:1773	the osteoblasts/osteoclasts activity ratio	1732:1773	These influences on the osteoblasts/osteoclasts activity ratio may support scaffold-driven bone healing in the future.
33036488	9	59	theme	scaffold-driven	1787:1801	arg1	healing					1808:1814	scaffold-driven bone healing	1787:1814	scaffold-driven bone healing	1787:1814	These influences on the osteoblasts/osteoclasts activity ratio may support scaffold-driven bone healing in the future.
33036488	0	60	theme	Calcium	51:57	arg1	Phases					69:74	Calcium Phosphate Phases	51:74	Calcium Phosphate Phases	51:74	Hemocyanin Modification of Chitosan Scaffolds with Calcium Phosphate Phases Increase the Osteoblast/Osteoclast Activity Ratio-A Co-Culture Study.
33036488	1	61	theme	cells	338:342	arg1	balance					315:321	balance	315:321	balance	315:321	The ongoing research on biomaterials that support bone regeneration led to the quest for materials or material modifications that can actively influence the activity or balance of bone tissue cells.
33036488	1	61	theme	cells	338:342	arg1	activity					303:310	activity	303:310	activity	303:310	The ongoing research on biomaterials that support bone regeneration led to the quest for materials or material modifications that can actively influence the activity or balance of bone tissue cells.
33036488	2	62	theme	hemocyanin	475:484	arg1	addition					441:448	the addition	437:448	the addition of calcium phosphates or hemocyanin	437:484	The bone biocompatibility of porous chitosan scaffolds was modified in the present study by the addition of calcium phosphates or hemocyanin.
33036488	6	63	theme	reduced	1096:1102	arg1	concentration					1112:1124	reduced calcium concentration	1096:1124	reduced calcium concentration in the cell culture medium	1096:1151	Mineral incorporation caused an increase in scaffold bioactivity, as shown by reduced calcium concentration in the cell culture medium, delayed differentiation of hBMSCs, and reduced osteoclastic maturation of hMs in mono-culture.
33036488	0	64	with	Modification	11:22	arg1	Phases					69:74	Calcium Phosphate Phases	51:74	Calcium Phosphate Phases	51:74	Hemocyanin Modification of Chitosan Scaffolds with Calcium Phosphate Phases Increase the Osteoblast/Osteoclast Activity Ratio-A Co-Culture Study.
33036488	9	65	from	influences	1718:1727	arg1	ratio					1769:1773	the osteoblasts/osteoclasts activity ratio	1732:1773	the osteoblasts/osteoclasts activity ratio	1732:1773	These influences on the osteoblasts/osteoclasts activity ratio may support scaffold-driven bone healing in the future.
33036488	0	66	mod	Modification	11:22	arg3	Hemocyanin					0:9	Hemocyanin Modification	0:22	Hemocyanin Modification of Chitosan Scaffolds with Calcium Phosphate Phases	0:74	Hemocyanin Modification of Chitosan Scaffolds with Calcium Phosphate Phases Increase the Osteoblast/Osteoclast Activity Ratio-A Co-Culture Study.
33036488	0	66	mod	Modification	11:22	arg1	Scaffolds					36:44	Chitosan Scaffolds	27:44	Chitosan Scaffolds	27:44	Hemocyanin Modification of Chitosan Scaffolds with Calcium Phosphate Phases Increase the Osteoblast/Osteoclast Activity Ratio-A Co-Culture Study.
33036488	7	67	from	proliferation	1294:1306	arg1	mono-culture					1358:1369	mono-culture	1358:1369	mono-culture	1358:1369	Dip-coating with hemocyanin led to increased proliferation of hBMSCs and equivalent osteoclast maturation in mono-culture, while in co-culture, both an inhibitory effect of mineral incorporation on osteoblastogenesis and stimulatory effects of hemocyanin were observed.
33036488	9	68	theme	bone	1803:1806	arg1	healing					1808:1814	scaffold-driven bone healing	1787:1814	scaffold-driven bone healing	1787:1814	These influences on the osteoblasts/osteoclasts activity ratio may support scaffold-driven bone healing in the future.
33036488	1	69	theme	ongoing	150:156	arg1	research					158:165	The ongoing research	146:165	The ongoing research on biomaterials that support bone regeneration	146:212	The ongoing research on biomaterials that support bone regeneration led to the quest for materials or material modifications that can actively influence the activity or balance of bone tissue cells.
33036488	5	70	theme	hBMSC/osteoblasts-hM/osteoclasts	984:1015	arg1	co-culture					970:979	a co-culture	968:979	a co-culture of hBMSC/osteoblasts-hM/osteoclasts	968:1015	The cytocompatibility was assessed in a mono-culture of human bone marrow stromal cells (hBMSCs) and their differentiation to osteoblasts; in a mono-culture of human monocytes (hMs) and their maturation to osteoclasts; and in a co-culture of hBMSC/osteoblasts-hM/osteoclasts.
33036488	6	71	theme	hBMSCs	1181:1186	arg1	differentiation					1162:1176	differentiation	1162:1176	differentiation of hBMSCs	1162:1186	Mineral incorporation caused an increase in scaffold bioactivity, as shown by reduced calcium concentration in the cell culture medium, delayed differentiation of hBMSCs, and reduced osteoclastic maturation of hMs in mono-culture.
33036488	6	72	from	maturation	1214:1223	arg1	mono-culture					1235:1246	mono-culture	1235:1246	mono-culture	1235:1246	Mineral incorporation caused an increase in scaffold bioactivity, as shown by reduced calcium concentration in the cell culture medium, delayed differentiation of hBMSCs, and reduced osteoclastic maturation of hMs in mono-culture.
33036488	1	73	theme	material	248:255	arg1	modifications					257:269	material modifications	248:269	material modifications	248:269	The ongoing research on biomaterials that support bone regeneration led to the quest for materials or material modifications that can actively influence the activity or balance of bone tissue cells.
33036488	4	74	with	scaffolds	681:689	arg1	hemocyanin					696:705	hemocyanin	696:705	hemocyanin extracted from crayfish hemolymph	696:739	The second strategy comprised dip-coating of chitosan scaffolds with hemocyanin extracted from crayfish hemolymph.
33036488	7	75	theme	mineral	1422:1428	arg1	incorporation					1430:1442	mineral incorporation	1422:1442	mineral incorporation	1422:1442	Dip-coating with hemocyanin led to increased proliferation of hBMSCs and equivalent osteoclast maturation in mono-culture, while in co-culture, both an inhibitory effect of mineral incorporation on osteoblastogenesis and stimulatory effects of hemocyanin were observed.
33036488	3	76	theme	phosphates	545:554	arg1	incorporation					520:532	the incorporation	516:532	the incorporation of calcium phosphates into chitosan	516:568	The first strategy comprised the incorporation of calcium phosphates into chitosan to create a biomimetic chitosan-mineral phase composite.
33036488	4	77	theme	chitosan	672:679	arg1	scaffolds					681:689	chitosan scaffolds	672:689	chitosan scaffolds with hemocyanin extracted from crayfish hemolymph	672:739	The second strategy comprised dip-coating of chitosan scaffolds with hemocyanin extracted from crayfish hemolymph.
33036488	7	78	theme	hBMSCs	1311:1316	arg1	proliferation					1294:1306	increased proliferation	1284:1306	increased proliferation of hBMSCs	1284:1316	Dip-coating with hemocyanin led to increased proliferation of hBMSCs and equivalent osteoclast maturation in mono-culture, while in co-culture, both an inhibitory effect of mineral incorporation on osteoblastogenesis and stimulatory effects of hemocyanin were observed.
33036488	7	78	theme	hBMSCs	1311:1316	arg1	maturation					1344:1353	equivalent osteoclast maturation	1322:1353	equivalent osteoclast maturation in mono-culture	1322:1369	Dip-coating with hemocyanin led to increased proliferation of hBMSCs and equivalent osteoclast maturation in mono-culture, while in co-culture, both an inhibitory effect of mineral incorporation on osteoblastogenesis and stimulatory effects of hemocyanin were observed.
33036488	6	79	theme	osteoclastic	1201:1212	arg1	maturation					1214:1223	osteoclastic maturation	1201:1223	osteoclastic maturation of hMs in mono-culture	1201:1246	Mineral incorporation caused an increase in scaffold bioactivity, as shown by reduced calcium concentration in the cell culture medium, delayed differentiation of hBMSCs, and reduced osteoclastic maturation of hMs in mono-culture.
33036488	7	80	theme	incorporation	1430:1442	arg1	effects					1482:1488	stimulatory effects	1470:1488	stimulatory effects of hemocyanin	1470:1502	Dip-coating with hemocyanin led to increased proliferation of hBMSCs and equivalent osteoclast maturation in mono-culture, while in co-culture, both an inhibitory effect of mineral incorporation on osteoblastogenesis and stimulatory effects of hemocyanin were observed.
33036488	7	80	theme	incorporation	1430:1442	arg1	effect					1412:1417	an inhibitory effect	1398:1417	an inhibitory effect of mineral incorporation on osteoblastogenesis	1398:1464	Dip-coating with hemocyanin led to increased proliferation of hBMSCs and equivalent osteoclast maturation in mono-culture, while in co-culture, both an inhibitory effect of mineral incorporation on osteoblastogenesis and stimulatory effects of hemocyanin were observed.
33036488	7	81	from	effect	1412:1417	arg1	osteoblastogenesis					1447:1464	osteoblastogenesis	1447:1464	osteoblastogenesis	1447:1464	Dip-coating with hemocyanin led to increased proliferation of hBMSCs and equivalent osteoclast maturation in mono-culture, while in co-culture, both an inhibitory effect of mineral incorporation on osteoblastogenesis and stimulatory effects of hemocyanin were observed.
33036488	2	82	theme	scaffolds	390:398	arg1	biocompatibility					354:369	The bone biocompatibility	345:369	The bone biocompatibility of porous chitosan scaffolds	345:398	The bone biocompatibility of porous chitosan scaffolds was modified in the present study by the addition of calcium phosphates or hemocyanin.
33036488	8	83	dep	scaffolds	1558:1566	arg1	containing					1569:1578	containing	1569:1578	containing mineral phases	1569:1593	It was concluded that highly bioactive scaffolds (containing mineral phases) restrain osteoblast and osteoclast development, while hemocyanin coating significantly supports osteoblastogenesis.
34500607	1	0	theme	unique	302:307	arg1	absorbent					349:357	a unique, multifunctional LS-AC-SG nanocomposite absorbent	300:357	a unique, multifunctional LS-AC-SG nanocomposite absorbent	300:357	Based on the adsorption performance of a porous nanocomposite with limestone (LS), activated carbon (AC) and sodium alginate (SG), a unique, multifunctional LS-AC-SG nanocomposite absorbent was designed and prepared for extracting antibiotics and drugs from aqueous solutions.
34500607	1	1	theme	activated	252:260	arg1	carbon					262:267	activated carbon	252:267	activated carbon (AC)	252:272	Based on the adsorption performance of a porous nanocomposite with limestone (LS), activated carbon (AC) and sodium alginate (SG), a unique, multifunctional LS-AC-SG nanocomposite absorbent was designed and prepared for extracting antibiotics and drugs from aqueous solutions.
34500607	1	1	theme	activated	252:260	arg1	AC					270:271	AC	270:271	AC	270:271	Based on the adsorption performance of a porous nanocomposite with limestone (LS), activated carbon (AC) and sodium alginate (SG), a unique, multifunctional LS-AC-SG nanocomposite absorbent was designed and prepared for extracting antibiotics and drugs from aqueous solutions.
34500607	2	2	theme	following	474:482	arg1	advantages					484:493	the following advantages	470:493	the following advantages: quick and simple	470:511	The composite exhibited the following advantages: quick and simple to prepare, multifunctionality and high efficiency.
34500607	1	3	theme	aqueous	427:433	arg1	solutions					435:443	aqueous solutions	427:443	aqueous solutions	427:443	Based on the adsorption performance of a porous nanocomposite with limestone (LS), activated carbon (AC) and sodium alginate (SG), a unique, multifunctional LS-AC-SG nanocomposite absorbent was designed and prepared for extracting antibiotics and drugs from aqueous solutions.
34500607	4	4	theme	characterization	769:784	arg1	methods					786:792	numerous characterization methods	760:792	numerous characterization methods	760:792	The prepared nanocomposite's physicochemical characteristics were calculated through numerous characterization methods.
34500607	0	5	theme	Aqueous	81:87	arg1	Solutions					89:97	Aqueous Solutions	81:97	Aqueous Solutions Using an Effective Limestone-Activated Carbon-Alginate Nanocomposite	81:166	An Efficient Strategy for Enhancing the Adsorption of Antibiotics and Drugs from Aqueous Solutions Using an Effective Limestone-Activated Carbon-Alginate Nanocomposite.
34500607	1	6	theme	LS-AC-SG	326:333	arg1	absorbent					349:357	a unique, multifunctional LS-AC-SG nanocomposite absorbent	300:357	a unique, multifunctional LS-AC-SG nanocomposite absorbent	300:357	Based on the adsorption performance of a porous nanocomposite with limestone (LS), activated carbon (AC) and sodium alginate (SG), a unique, multifunctional LS-AC-SG nanocomposite absorbent was designed and prepared for extracting antibiotics and drugs from aqueous solutions.
34500607	7	7	theme	contact	1089:1095	arg1	time					1097:1100	a contact time	1087:1100	a contact time of 40 min	1087:1110	The results show that the maximum absorption capacity of amoxicillin and diclofenac was 99.6% and 98.4%, respectively, at a contact time of 40 min.
34500607	10	8	theme	synthesized	1357:1367	arg1	nanocomposites					1369:1382	the synthesized nanocomposites	1353:1382	the synthesized nanocomposites	1353:1382	The results imply that the synthesized nanocomposites have the capacity to remove amoxicillin (AMX) and diclofenac (DCF) from aqueous solutions.
34500607	4	9	theme	numerous	760:767	arg1	methods					786:792	numerous characterization methods	760:792	numerous characterization methods	760:792	The prepared nanocomposite's physicochemical characteristics were calculated through numerous characterization methods.
34500607	2	10	theme	simple	506:511	arg1	advantages					484:493	the following advantages	470:493	the following advantages: quick and simple	470:511	The composite exhibited the following advantages: quick and simple to prepare, multifunctionality and high efficiency.
34500607	1	11	theme	nanocomposite	335:347	arg1	absorbent					349:357	a unique, multifunctional LS-AC-SG nanocomposite absorbent	300:357	a unique, multifunctional LS-AC-SG nanocomposite absorbent	300:357	Based on the adsorption performance of a porous nanocomposite with limestone (LS), activated carbon (AC) and sodium alginate (SG), a unique, multifunctional LS-AC-SG nanocomposite absorbent was designed and prepared for extracting antibiotics and drugs from aqueous solutions.
34500607	5	12	theme	interconnected	839:852	arg1	pores					854:858	interconnected pores	839:858	interconnected pores that can easily confine pollutants	839:893	The structure of the surface was made up of interconnected pores that can easily confine pollutants.
34500607	1	13	theme	adsorption	182:191	arg1	performance					193:203	the adsorption performance	178:203	the adsorption performance of a porous nanocomposite with limestone (LS), activated carbon (AC) and sodium alginate (SG)	178:297	Based on the adsorption performance of a porous nanocomposite with limestone (LS), activated carbon (AC) and sodium alginate (SG), a unique, multifunctional LS-AC-SG nanocomposite absorbent was designed and prepared for extracting antibiotics and drugs from aqueous solutions.
34500607	7	14	theme	min	1108:1110	arg1	time					1097:1100	a contact time	1087:1100	a contact time of 40 min	1087:1110	The results show that the maximum absorption capacity of amoxicillin and diclofenac was 99.6% and 98.4%, respectively, at a contact time of 40 min.
34500607	8	15	theme	amoxicillin	1136:1146	arg1	removal					1125:1131	The maximum removal	1113:1131	The maximum removal of amoxicillin and diclofenac	1113:1161	The maximum removal of amoxicillin and diclofenac was reached at pH = 2.
34500607	0	16	theme	Efficient	3:11	arg1	Strategy					13:20	An Efficient Strategy	0:20	An Efficient Strategy for Enhancing the Adsorption of Antibiotics and Drugs from Aqueous Solutions Using an Effective Limestone-Activated Carbon-Alginate Nanocomposite.	0:167	An Efficient Strategy for Enhancing the Adsorption of Antibiotics and Drugs from Aqueous Solutions Using an Effective Limestone-Activated Carbon-Alginate Nanocomposite.
34500607	0	17	theme	Drugs	70:74	arg1	Adsorption					40:49	the Adsorption	36:49	the Adsorption of Antibiotics and Drugs from Aqueous Solutions Using an Effective Limestone-Activated Carbon-Alginate Nanocomposite	36:166	An Efficient Strategy for Enhancing the Adsorption of Antibiotics and Drugs from Aqueous Solutions Using an Effective Limestone-Activated Carbon-Alginate Nanocomposite.
34500607	6	18	theme	surface	900:906	arg1	area					908:911	The surface area	896:911	The surface area	896:911	The surface area was measured to be 27.85 m2/g through BET analysis.
34500607	6	18	theme	surface	900:906	arg1	m2/g					938:941	27.85 m2/g	932:941	27.85 m2/g	932:941	The surface area was measured to be 27.85 m2/g through BET analysis.
34500607	9	19	theme	Langmuir	1304:1311	arg1	isotherm					1313:1320	the Langmuir isotherm	1300:1320	the Langmuir isotherm	1300:1320	Adsorption analysis revealed that adsorption isotherm and kinetic data matched the pseudo-first-order kinetic and the Langmuir isotherm models.
34500607	9	20	dep	kinetic	1288:1294	arg1	models					1322:1327	models	1322:1327	models	1322:1327	Adsorption analysis revealed that adsorption isotherm and kinetic data matched the pseudo-first-order kinetic and the Langmuir isotherm models.
34500607	2	21	theme	high	548:551	arg1	efficiency					553:562	high efficiency	548:562	high efficiency	548:562	The composite exhibited the following advantages: quick and simple to prepare, multifunctionality and high efficiency.
34500607	1	22	theme	porous	210:215	arg1	nanocomposite					217:229	a porous nanocomposite	208:229	a porous nanocomposite with limestone (LS), activated carbon (AC) and sodium alginate (SG)	208:297	Based on the adsorption performance of a porous nanocomposite with limestone (LS), activated carbon (AC) and sodium alginate (SG), a unique, multifunctional LS-AC-SG nanocomposite absorbent was designed and prepared for extracting antibiotics and drugs from aqueous solutions.
34500607	1	23	theme	sodium	278:283	arg1	SG					295:296	SG	295:296	SG	295:296	Based on the adsorption performance of a porous nanocomposite with limestone (LS), activated carbon (AC) and sodium alginate (SG), a unique, multifunctional LS-AC-SG nanocomposite absorbent was designed and prepared for extracting antibiotics and drugs from aqueous solutions.
34500607	1	23	theme	sodium	278:283	arg1	alginate					285:292	sodium alginate	278:292	sodium alginate (SG)	278:297	Based on the adsorption performance of a porous nanocomposite with limestone (LS), activated carbon (AC) and sodium alginate (SG), a unique, multifunctional LS-AC-SG nanocomposite absorbent was designed and prepared for extracting antibiotics and drugs from aqueous solutions.
34500607	0	24	theme	Effective	108:116	arg1	Nanocomposite					154:166	an Effective Limestone-Activated Carbon-Alginate Nanocomposite	105:166	an Effective Limestone-Activated Carbon-Alginate Nanocomposite	105:166	An Efficient Strategy for Enhancing the Adsorption of Antibiotics and Drugs from Aqueous Solutions Using an Effective Limestone-Activated Carbon-Alginate Nanocomposite.
34500607	1	25	theme	nanocomposite	217:229	arg1	performance					193:203	the adsorption performance	178:203	the adsorption performance of a porous nanocomposite with limestone (LS), activated carbon (AC) and sodium alginate (SG)	178:297	Based on the adsorption performance of a porous nanocomposite with limestone (LS), activated carbon (AC) and sodium alginate (SG), a unique, multifunctional LS-AC-SG nanocomposite absorbent was designed and prepared for extracting antibiotics and drugs from aqueous solutions.
34500607	1	26	dep	unique	302:307	arg1	multifunctional					310:324	multifunctional	310:324	multifunctional	310:324	Based on the adsorption performance of a porous nanocomposite with limestone (LS), activated carbon (AC) and sodium alginate (SG), a unique, multifunctional LS-AC-SG nanocomposite absorbent was designed and prepared for extracting antibiotics and drugs from aqueous solutions.
34500607	9	27	theme	Adsorption	1186:1195	arg1	analysis					1197:1204	Adsorption analysis	1186:1204	Adsorption analysis	1186:1204	Adsorption analysis revealed that adsorption isotherm and kinetic data matched the pseudo-first-order kinetic and the Langmuir isotherm models.
34500607	6	28	theme	BET	951:953	arg1	analysis					955:962	BET analysis	951:962	BET analysis	951:962	The surface area was measured to be 27.85 m2/g through BET analysis.
34500607	0	29	theme	Carbon-Alginate	138:152	arg1	Nanocomposite					154:166	an Effective Limestone-Activated Carbon-Alginate Nanocomposite	105:166	an Effective Limestone-Activated Carbon-Alginate Nanocomposite	105:166	An Efficient Strategy for Enhancing the Adsorption of Antibiotics and Drugs from Aqueous Solutions Using an Effective Limestone-Activated Carbon-Alginate Nanocomposite.
34500607	8	30	theme	diclofenac	1152:1161	arg1	removal					1125:1131	The maximum removal	1113:1131	The maximum removal of amoxicillin and diclofenac	1113:1161	The maximum removal of amoxicillin and diclofenac was reached at pH = 2.
34500607	7	31	theme	amoxicillin	1022:1032	arg1	capacity					1010:1017	the maximum absorption capacity	987:1017	the maximum absorption capacity of amoxicillin and diclofenac	987:1047	The results show that the maximum absorption capacity of amoxicillin and diclofenac was 99.6% and 98.4%, respectively, at a contact time of 40 min.
34500607	7	31	theme	amoxicillin	1022:1032	arg1	%					1057:1057	99.6%	1053:1057	99.6%	1053:1057	The results show that the maximum absorption capacity of amoxicillin and diclofenac was 99.6% and 98.4%, respectively, at a contact time of 40 min.
34500607	1	32	with	nanocomposite	217:229	arg1	SG					295:296	SG	295:296	SG	295:296	Based on the adsorption performance of a porous nanocomposite with limestone (LS), activated carbon (AC) and sodium alginate (SG), a unique, multifunctional LS-AC-SG nanocomposite absorbent was designed and prepared for extracting antibiotics and drugs from aqueous solutions.
34500607	1	32	with	nanocomposite	217:229	arg1	LS					247:248	LS	247:248	LS	247:248	Based on the adsorption performance of a porous nanocomposite with limestone (LS), activated carbon (AC) and sodium alginate (SG), a unique, multifunctional LS-AC-SG nanocomposite absorbent was designed and prepared for extracting antibiotics and drugs from aqueous solutions.
34500607	1	32	with	nanocomposite	217:229	arg1	alginate					285:292	sodium alginate	278:292	sodium alginate (SG)	278:297	Based on the adsorption performance of a porous nanocomposite with limestone (LS), activated carbon (AC) and sodium alginate (SG), a unique, multifunctional LS-AC-SG nanocomposite absorbent was designed and prepared for extracting antibiotics and drugs from aqueous solutions.
34500607	1	32	with	nanocomposite	217:229	arg1	limestone					236:244	limestone	236:244	limestone (LS)	236:249	Based on the adsorption performance of a porous nanocomposite with limestone (LS), activated carbon (AC) and sodium alginate (SG), a unique, multifunctional LS-AC-SG nanocomposite absorbent was designed and prepared for extracting antibiotics and drugs from aqueous solutions.
34500607	1	32	with	nanocomposite	217:229	arg1	carbon					262:267	activated carbon	252:267	activated carbon (AC)	252:272	Based on the adsorption performance of a porous nanocomposite with limestone (LS), activated carbon (AC) and sodium alginate (SG), a unique, multifunctional LS-AC-SG nanocomposite absorbent was designed and prepared for extracting antibiotics and drugs from aqueous solutions.
34500607	1	32	with	nanocomposite	217:229	arg1	AC					270:271	AC	270:271	AC	270:271	Based on the adsorption performance of a porous nanocomposite with limestone (LS), activated carbon (AC) and sodium alginate (SG), a unique, multifunctional LS-AC-SG nanocomposite absorbent was designed and prepared for extracting antibiotics and drugs from aqueous solutions.
34500607	7	33	from	time	1097:1100	arg1	capacity					1010:1017	the maximum absorption capacity	987:1017	the maximum absorption capacity of amoxicillin and diclofenac	987:1047	The results show that the maximum absorption capacity of amoxicillin and diclofenac was 99.6% and 98.4%, respectively, at a contact time of 40 min.
34500607	7	33	from	time	1097:1100	arg1	%					1057:1057	99.6%	1053:1057	99.6%	1053:1057	The results show that the maximum absorption capacity of amoxicillin and diclofenac was 99.6% and 98.4%, respectively, at a contact time of 40 min.
34500607	7	33	from	time	1097:1100	arg1	%					1067:1067	98.4%	1063:1067	98.4%	1063:1067	The results show that the maximum absorption capacity of amoxicillin and diclofenac was 99.6% and 98.4%, respectively, at a contact time of 40 min.
34500607	0	34	theme	Limestone-Activated	118:136	arg1	Nanocomposite					154:166	an Effective Limestone-Activated Carbon-Alginate Nanocomposite	105:166	an Effective Limestone-Activated Carbon-Alginate Nanocomposite	105:166	An Efficient Strategy for Enhancing the Adsorption of Antibiotics and Drugs from Aqueous Solutions Using an Effective Limestone-Activated Carbon-Alginate Nanocomposite.
34500607	7	35	theme	absorption	999:1008	arg1	capacity					1010:1017	the maximum absorption capacity	987:1017	the maximum absorption capacity of amoxicillin and diclofenac	987:1047	The results show that the maximum absorption capacity of amoxicillin and diclofenac was 99.6% and 98.4%, respectively, at a contact time of 40 min.
34500607	7	35	theme	absorption	999:1008	arg1	%					1057:1057	99.6%	1053:1057	99.6%	1053:1057	The results show that the maximum absorption capacity of amoxicillin and diclofenac was 99.6% and 98.4%, respectively, at a contact time of 40 min.
34500607	4	36	theme	prepared	679:686	arg1	characteristics					720:734	The prepared nanocomposite's physicochemical characteristics	675:734	The prepared nanocomposite's physicochemical characteristics	675:734	The prepared nanocomposite's physicochemical characteristics were calculated through numerous characterization methods.
34500607	6	37	theme	27.85	932:936	arg1	area					908:911	The surface area	896:911	The surface area	896:911	The surface area was measured to be 27.85 m2/g through BET analysis.
34500607	6	37	theme	27.85	932:936	arg1	m2/g					938:941	27.85 m2/g	932:941	27.85 m2/g	932:941	The surface area was measured to be 27.85 m2/g through BET analysis.
34500607	9	38	theme	adsorption	1220:1229	arg1	isotherm					1231:1238	adsorption isotherm	1220:1238	adsorption isotherm	1220:1238	Adsorption analysis revealed that adsorption isotherm and kinetic data matched the pseudo-first-order kinetic and the Langmuir isotherm models.
34500607	4	39	theme	physicochemical	704:718	arg1	characteristics					720:734	The prepared nanocomposite's physicochemical characteristics	675:734	The prepared nanocomposite's physicochemical characteristics	675:734	The prepared nanocomposite's physicochemical characteristics were calculated through numerous characterization methods.
34500607	7	40	theme	diclofenac	1038:1047	arg1	capacity					1010:1017	the maximum absorption capacity	987:1017	the maximum absorption capacity of amoxicillin and diclofenac	987:1047	The results show that the maximum absorption capacity of amoxicillin and diclofenac was 99.6% and 98.4%, respectively, at a contact time of 40 min.
34500607	7	40	theme	diclofenac	1038:1047	arg1	%					1057:1057	99.6%	1053:1057	99.6%	1053:1057	The results show that the maximum absorption capacity of amoxicillin and diclofenac was 99.6% and 98.4%, respectively, at a contact time of 40 min.
34500607	0	41	from	Solutions	89:97	arg1	Adsorption					40:49	the Adsorption	36:49	the Adsorption of Antibiotics and Drugs from Aqueous Solutions Using an Effective Limestone-Activated Carbon-Alginate Nanocomposite	36:166	An Efficient Strategy for Enhancing the Adsorption of Antibiotics and Drugs from Aqueous Solutions Using an Effective Limestone-Activated Carbon-Alginate Nanocomposite.
34500607	10	42	attach	remove	1405:1410	arg2	capacity					1393:1400	the capacity to remove amoxicillin (AMX) and diclofenac (DCF) from aqueous solutions	1389:1472	the capacity to remove amoxicillin (AMX) and diclofenac (DCF) from aqueous solutions	1389:1472	The results imply that the synthesized nanocomposites have the capacity to remove amoxicillin (AMX) and diclofenac (DCF) from aqueous solutions.
34500607	10	42	attach	remove	1405:1410	arg1	solutions					1464:1472	aqueous solutions	1456:1472	aqueous solutions	1456:1472	The results imply that the synthesized nanocomposites have the capacity to remove amoxicillin (AMX) and diclofenac (DCF) from aqueous solutions.
34500607	2	43	theme	quick	496:500	arg1	advantages					484:493	the following advantages	470:493	the following advantages: quick and simple	470:511	The composite exhibited the following advantages: quick and simple to prepare, multifunctionality and high efficiency.
34500607	7	44	theme	maximum	991:997	arg1	capacity					1010:1017	the maximum absorption capacity	987:1017	the maximum absorption capacity of amoxicillin and diclofenac	987:1047	The results show that the maximum absorption capacity of amoxicillin and diclofenac was 99.6% and 98.4%, respectively, at a contact time of 40 min.
34500607	7	44	theme	maximum	991:997	arg1	%					1057:1057	99.6%	1053:1057	99.6%	1053:1057	The results show that the maximum absorption capacity of amoxicillin and diclofenac was 99.6% and 98.4%, respectively, at a contact time of 40 min.
34500607	10	45	theme	aqueous	1456:1462	arg1	solutions					1464:1472	aqueous solutions	1456:1472	aqueous solutions	1456:1472	The results imply that the synthesized nanocomposites have the capacity to remove amoxicillin (AMX) and diclofenac (DCF) from aqueous solutions.
34500607	0	46	theme	Antibiotics	54:64	arg1	Adsorption					40:49	the Adsorption	36:49	the Adsorption of Antibiotics and Drugs from Aqueous Solutions Using an Effective Limestone-Activated Carbon-Alginate Nanocomposite	36:166	An Efficient Strategy for Enhancing the Adsorption of Antibiotics and Drugs from Aqueous Solutions Using an Effective Limestone-Activated Carbon-Alginate Nanocomposite.
34500607	9	47	theme	kinetic	1244:1250	arg1	data					1252:1255	kinetic data	1244:1255	kinetic data	1244:1255	Adsorption analysis revealed that adsorption isotherm and kinetic data matched the pseudo-first-order kinetic and the Langmuir isotherm models.
34500607	10	48	contain	have	1384:1387	arg1	nanocomposites					1369:1382	the synthesized nanocomposites	1353:1382	the synthesized nanocomposites	1353:1382	The results imply that the synthesized nanocomposites have the capacity to remove amoxicillin (AMX) and diclofenac (DCF) from aqueous solutions.
34500607	10	48	contain	have	1384:1387	arg2	capacity					1393:1400	the capacity to remove amoxicillin (AMX) and diclofenac (DCF) from aqueous solutions	1389:1472	the capacity to remove amoxicillin (AMX) and diclofenac (DCF) from aqueous solutions	1389:1472	The results imply that the synthesized nanocomposites have the capacity to remove amoxicillin (AMX) and diclofenac (DCF) from aqueous solutions.
34500607	5	49	theme	surface	816:822	arg1	structure					799:807	The structure	795:807	The structure of the surface	795:822	The structure of the surface was made up of interconnected pores that can easily confine pollutants.
34500607	8	50	theme	maximum	1117:1123	arg1	removal					1125:1131	The maximum removal	1113:1131	The maximum removal of amoxicillin and diclofenac	1113:1161	The maximum removal of amoxicillin and diclofenac was reached at pH = 2.
34444972	0	0	theme	Monocytes	87:95	arg1	Migration					53:61	the Migration	49:61	the Migration of Colonic Inflammatory Monocytes and Diet-Induced Metabolic Syndrome	49:131	Seaweed Dietary Fiber Sodium Alginate Suppresses the Migration of Colonic Inflammatory Monocytes and Diet-Induced Metabolic Syndrome via the Gut Microbiota.
34444972	4	1	from	effect	598:603	arg1	microbiota					616:625	the gut microbiota	608:625	the gut microbiota	608:625	Sodium alginate (SA) is a seaweed-derived dietary fiber that suppresses high-fat diet (HFD)-induced MetS via an effect on the gut microbiota.
34444972	9	2	theme	inflammatory	1188:1199	arg1	monocytes					1201:1209	inflammatory monocytes	1188:1209	inflammatory monocytes	1188:1209	The abundance of Bacteroides was negatively correlated with that of inflammatory monocytes and positively correlated with the levels of several gut metabolites.
34444972	7	3	theme	Antibiotic	893:902	arg1	treatment					904:912	Antibiotic treatment	893:912	Antibiotic treatment	893:912	Antibiotic treatment completely abolished the suppressive effects of SA on MetS.
34444972	1	4	theme	billion	261:267	arg1	worldwide					276:284	approximately one billion people worldwide	243:284	approximately one billion people worldwide	243:284	Metabolic syndrome (MetS) is a multifactorial chronic metabolic disorder that affects approximately one billion people worldwide.
34444972	0	5	theme	Inflammatory	74:85	arg1	Monocytes					87:95	Colonic Inflammatory Monocytes	66:95	Colonic Inflammatory Monocytes	66:95	Seaweed Dietary Fiber Sodium Alginate Suppresses the Migration of Colonic Inflammatory Monocytes and Diet-Induced Metabolic Syndrome via the Gut Microbiota.
34444972	4	6	theme	seaweed-derived	512:526	arg1	fiber					536:540	a seaweed-derived dietary fiber	510:540	a seaweed-derived dietary fiber that suppresses high-fat diet (HFD)-induced MetS via an effect on the gut microbiota	510:625	Sodium alginate (SA) is a seaweed-derived dietary fiber that suppresses high-fat diet (HFD)-induced MetS via an effect on the gut microbiota.
34444972	4	6	theme	seaweed-derived	512:526	arg1	alginate					493:500	Sodium alginate	486:500	Sodium alginate (SA)	486:505	Sodium alginate (SA) is a seaweed-derived dietary fiber that suppresses high-fat diet (HFD)-induced MetS via an effect on the gut microbiota.
34444972	8	7	theme	monocytes	1039:1047	arg1	number					1008:1013	the number	1004:1013	the number	1004:1013	Mechanistically, SA decreased the number of colonic inflammatory monocytes, which promote MetS development, in a gut microbiota-dependent manner.
34444972	10	8	theme	present	1285:1291	arg1	study					1293:1297	The present study	1281:1297	The present study	1281:1297	The present study revealed a novel food function of SA in preventing HFD-induced MetS through its action on gut microbiota.
34444972	1	9	theme	people	269:274	arg1	worldwide					276:284	approximately one billion people worldwide	243:284	approximately one billion people worldwide	243:284	Metabolic syndrome (MetS) is a multifactorial chronic metabolic disorder that affects approximately one billion people worldwide.
34444972	0	10	theme	Diet-Induced	101:112	arg1	Syndrome					124:131	Diet-Induced Metabolic Syndrome	101:131	Diet-Induced Metabolic Syndrome	101:131	Seaweed Dietary Fiber Sodium Alginate Suppresses the Migration of Colonic Inflammatory Monocytes and Diet-Induced Metabolic Syndrome via the Gut Microbiota.
34444972	5	11	theme	SA	645:646	arg1	supplementation					648:662	SA supplementation	645:662	SA supplementation	645:662	We observed that SA supplementation significantly decreased body weight gain, cholesterol levels, and fat weight, while improving glucose tolerance in HFD-fed mice.
34444972	0	12	theme	Syndrome	124:131	arg1	Migration					53:61	the Migration	49:61	the Migration of Colonic Inflammatory Monocytes and Diet-Induced Metabolic Syndrome	49:131	Seaweed Dietary Fiber Sodium Alginate Suppresses the Migration of Colonic Inflammatory Monocytes and Diet-Induced Metabolic Syndrome via the Gut Microbiota.
34444972	4	13	theme	Sodium	486:491	arg1	SA					503:504	SA	503:504	SA	503:504	Sodium alginate (SA) is a seaweed-derived dietary fiber that suppresses high-fat diet (HFD)-induced MetS via an effect on the gut microbiota.
34444972	4	13	theme	Sodium	486:491	arg1	alginate					493:500	Sodium alginate	486:500	Sodium alginate (SA)	486:505	Sodium alginate (SA) is a seaweed-derived dietary fiber that suppresses high-fat diet (HFD)-induced MetS via an effect on the gut microbiota.
34444972	4	13	theme	Sodium	486:491	arg1	fiber					536:540	a seaweed-derived dietary fiber	510:540	a seaweed-derived dietary fiber that suppresses high-fat diet (HFD)-induced MetS via an effect on the gut microbiota	510:625	Sodium alginate (SA) is a seaweed-derived dietary fiber that suppresses high-fat diet (HFD)-induced MetS via an effect on the gut microbiota.
34444972	4	14	theme	gut	612:614	arg1	microbiota					616:625	the gut microbiota	608:625	the gut microbiota	608:625	Sodium alginate (SA) is a seaweed-derived dietary fiber that suppresses high-fat diet (HFD)-induced MetS via an effect on the gut microbiota.
34444972	10	15	theme	food	1316:1319	arg1	function					1321:1328	a novel food function	1308:1328	a novel food function of SA in preventing HFD-induced MetS through its action on gut microbiota	1308:1402	The present study revealed a novel food function of SA in preventing HFD-induced MetS through its action on gut microbiota.
34444972	0	16	theme	Metabolic	114:122	arg1	Syndrome					124:131	Diet-Induced Metabolic Syndrome	101:131	Diet-Induced Metabolic Syndrome	101:131	Seaweed Dietary Fiber Sodium Alginate Suppresses the Migration of Colonic Inflammatory Monocytes and Diet-Induced Metabolic Syndrome via the Gut Microbiota.
34444972	6	17	theme	Bacteroides	880:890	arg1	abundance					867:875	the abundance	863:875	the abundance of Bacteroides	863:890	SA changed the gut microbiota composition and significantly increased the abundance of Bacteroides.
34444972	4	18	theme	-induced	577:584	arg1	MetS					586:589	high-fat diet (HFD)-induced MetS	558:589	high-fat diet (HFD)-induced MetS	558:589	Sodium alginate (SA) is a seaweed-derived dietary fiber that suppresses high-fat diet (HFD)-induced MetS via an effect on the gut microbiota.
34444972	9	19	theme	gut	1264:1266	arg1	metabolites					1268:1278	several gut metabolites	1256:1278	several gut metabolites	1256:1278	The abundance of Bacteroides was negatively correlated with that of inflammatory monocytes and positively correlated with the levels of several gut metabolites.
34444972	2	20	theme	Recent	287:292	arg1	studies					294:300	Recent studies	287:300	Recent studies	287:300	Recent studies have evaluated whether targeting the gut microbiota can prevent MetS.
34444972	10	21	theme	novel	1310:1314	arg1	function					1321:1328	a novel food function	1308:1328	a novel food function of SA in preventing HFD-induced MetS through its action on gut microbiota	1308:1402	The present study revealed a novel food function of SA in preventing HFD-induced MetS through its action on gut microbiota.
34444972	5	22	theme	fat	730:732	arg1	weight					734:739	fat weight	730:739	fat weight	730:739	We observed that SA supplementation significantly decreased body weight gain, cholesterol levels, and fat weight, while improving glucose tolerance in HFD-fed mice.
34444972	0	23	theme	Dietary	8:14	arg1	Alginate					29:36	Seaweed Dietary Fiber Sodium Alginate	0:36	Seaweed Dietary Fiber Sodium Alginate	0:36	Seaweed Dietary Fiber Sodium Alginate Suppresses the Migration of Colonic Inflammatory Monocytes and Diet-Induced Metabolic Syndrome via the Gut Microbiota.
34444972	5	24	theme	glucose	758:764	arg1	tolerance					766:774	glucose tolerance	758:774	glucose tolerance in HFD-fed mice	758:790	We observed that SA supplementation significantly decreased body weight gain, cholesterol levels, and fat weight, while improving glucose tolerance in HFD-fed mice.
34444972	7	25	theme	SA	962:963	arg1	effects					951:957	the suppressive effects	935:957	the suppressive effects of SA on MetS	935:971	Antibiotic treatment completely abolished the suppressive effects of SA on MetS.
34444972	2	26	theme	gut	339:341	arg1	microbiota					343:352	the gut microbiota	335:352	the gut microbiota	335:352	Recent studies have evaluated whether targeting the gut microbiota can prevent MetS.
34444972	0	27	theme	Seaweed	0:6	arg1	Alginate					29:36	Seaweed Dietary Fiber Sodium Alginate	0:36	Seaweed Dietary Fiber Sodium Alginate	0:36	Seaweed Dietary Fiber Sodium Alginate Suppresses the Migration of Colonic Inflammatory Monocytes and Diet-Induced Metabolic Syndrome via the Gut Microbiota.
34444972	4	28	link	seaweed-derived	512:526	arg1	fiber					536:540	a seaweed-derived dietary fiber	510:540	a seaweed-derived dietary fiber that suppresses high-fat diet (HFD)-induced MetS via an effect on the gut microbiota	510:625	Sodium alginate (SA) is a seaweed-derived dietary fiber that suppresses high-fat diet (HFD)-induced MetS via an effect on the gut microbiota.
34444972	4	28	link	seaweed-derived	512:526	arg1	alginate					493:500	Sodium alginate	486:500	Sodium alginate (SA)	486:505	Sodium alginate (SA) is a seaweed-derived dietary fiber that suppresses high-fat diet (HFD)-induced MetS via an effect on the gut microbiota.
34444972	7	29	theme	suppressive	939:949	arg1	effects					951:957	the suppressive effects	935:957	the suppressive effects of SA on MetS	935:971	Antibiotic treatment completely abolished the suppressive effects of SA on MetS.
34444972	8	30	theme	inflammatory	1026:1037	arg1	monocytes					1039:1047	colonic inflammatory monocytes	1018:1047	colonic inflammatory monocytes	1018:1047	Mechanistically, SA decreased the number of colonic inflammatory monocytes, which promote MetS development, in a gut microbiota-dependent manner.
34444972	0	31	theme	Sodium	22:27	arg1	Alginate					29:36	Seaweed Dietary Fiber Sodium Alginate	0:36	Seaweed Dietary Fiber Sodium Alginate	0:36	Seaweed Dietary Fiber Sodium Alginate Suppresses the Migration of Colonic Inflammatory Monocytes and Diet-Induced Metabolic Syndrome via the Gut Microbiota.
34444972	4	32	theme	high-fat	558:565	arg1	diet					567:570	high-fat diet	558:570	high-fat diet (HFD)	558:576	Sodium alginate (SA) is a seaweed-derived dietary fiber that suppresses high-fat diet (HFD)-induced MetS via an effect on the gut microbiota.
34444972	4	32	theme	high-fat	558:565	arg1	HFD					573:575	HFD	573:575	HFD	573:575	Sodium alginate (SA) is a seaweed-derived dietary fiber that suppresses high-fat diet (HFD)-induced MetS via an effect on the gut microbiota.
34444972	8	33	theme	gut	1087:1089	arg1	manner					1112:1117	a gut microbiota-dependent manner	1085:1117	a gut microbiota-dependent manner	1085:1117	Mechanistically, SA decreased the number of colonic inflammatory monocytes, which promote MetS development, in a gut microbiota-dependent manner.
34444972	8	34	theme	colonic	1018:1024	arg1	monocytes					1039:1047	colonic inflammatory monocytes	1018:1047	colonic inflammatory monocytes	1018:1047	Mechanistically, SA decreased the number of colonic inflammatory monocytes, which promote MetS development, in a gut microbiota-dependent manner.
34444972	1	35	theme	multifactorial	188:201	arg1	syndrome					167:174	Metabolic syndrome	157:174	Metabolic syndrome (MetS)	157:181	Metabolic syndrome (MetS) is a multifactorial chronic metabolic disorder that affects approximately one billion people worldwide.
34444972	1	35	theme	multifactorial	188:201	arg1	disorder					221:228	a multifactorial chronic metabolic disorder	186:228	a multifactorial chronic metabolic disorder that affects approximately one billion people worldwide	186:284	Metabolic syndrome (MetS) is a multifactorial chronic metabolic disorder that affects approximately one billion people worldwide.
34444972	0	36	theme	Fiber	16:20	arg1	Alginate					29:36	Seaweed Dietary Fiber Sodium Alginate	0:36	Seaweed Dietary Fiber Sodium Alginate	0:36	Seaweed Dietary Fiber Sodium Alginate Suppresses the Migration of Colonic Inflammatory Monocytes and Diet-Induced Metabolic Syndrome via the Gut Microbiota.
34444972	9	37	theme	several	1256:1262	arg1	metabolites					1268:1278	several gut metabolites	1256:1278	several gut metabolites	1256:1278	The abundance of Bacteroides was negatively correlated with that of inflammatory monocytes and positively correlated with the levels of several gut metabolites.
34444972	10	38	theme	SA	1333:1334	arg1	function					1321:1328	a novel food function	1308:1328	a novel food function of SA in preventing HFD-induced MetS through its action on gut microbiota	1308:1402	The present study revealed a novel food function of SA in preventing HFD-induced MetS through its action on gut microbiota.
34444972	1	39	theme	chronic	203:209	arg1	syndrome					167:174	Metabolic syndrome	157:174	Metabolic syndrome (MetS)	157:181	Metabolic syndrome (MetS) is a multifactorial chronic metabolic disorder that affects approximately one billion people worldwide.
34444972	1	39	theme	chronic	203:209	arg1	disorder					221:228	a multifactorial chronic metabolic disorder	186:228	a multifactorial chronic metabolic disorder that affects approximately one billion people worldwide	186:284	Metabolic syndrome (MetS) is a multifactorial chronic metabolic disorder that affects approximately one billion people worldwide.
34444972	3	40	theme	fiber	422:426	arg1	ability					403:409	the ability	399:409	the ability of dietary fiber to control MetS by modulating gut microbiota composition	399:483	This study aimed to assess the ability of dietary fiber to control MetS by modulating gut microbiota composition.
34444972	10	41	from	action	1379:1384	arg1	microbiota					1393:1402	gut microbiota	1389:1402	gut microbiota	1389:1402	The present study revealed a novel food function of SA in preventing HFD-induced MetS through its action on gut microbiota.
34444972	5	42	theme	cholesterol	706:716	arg1	levels					718:723	cholesterol levels	706:723	cholesterol levels	706:723	We observed that SA supplementation significantly decreased body weight gain, cholesterol levels, and fat weight, while improving glucose tolerance in HFD-fed mice.
34444972	1	43	theme	metabolic	211:219	arg1	syndrome					167:174	Metabolic syndrome	157:174	Metabolic syndrome (MetS)	157:181	Metabolic syndrome (MetS) is a multifactorial chronic metabolic disorder that affects approximately one billion people worldwide.
34444972	1	43	theme	metabolic	211:219	arg1	disorder					221:228	a multifactorial chronic metabolic disorder	186:228	a multifactorial chronic metabolic disorder that affects approximately one billion people worldwide	186:284	Metabolic syndrome (MetS) is a multifactorial chronic metabolic disorder that affects approximately one billion people worldwide.
34444972	3	44	theme	gut	458:460	arg1	composition					473:483	gut microbiota composition	458:483	gut microbiota composition	458:483	This study aimed to assess the ability of dietary fiber to control MetS by modulating gut microbiota composition.
34444972	0	45	theme	Gut	141:143	arg1	Microbiota					145:154	the Gut Microbiota	137:154	the Gut Microbiota	137:154	Seaweed Dietary Fiber Sodium Alginate Suppresses the Migration of Colonic Inflammatory Monocytes and Diet-Induced Metabolic Syndrome via the Gut Microbiota.
34444972	9	46	theme	metabolites	1268:1278	arg1	levels					1246:1251	the levels	1242:1251	the levels of several gut metabolites	1242:1278	The abundance of Bacteroides was negatively correlated with that of inflammatory monocytes and positively correlated with the levels of several gut metabolites.
34444972	9	47	theme	Bacteroides	1137:1147	arg1	abundance					1124:1132	The abundance	1120:1132	The abundance of Bacteroides	1120:1147	The abundance of Bacteroides was negatively correlated with that of inflammatory monocytes and positively correlated with the levels of several gut metabolites.
34444972	3	48	theme	microbiota	462:471	arg1	composition					473:483	gut microbiota composition	458:483	gut microbiota composition	458:483	This study aimed to assess the ability of dietary fiber to control MetS by modulating gut microbiota composition.
34444972	5	49	theme	HFD-fed	779:785	arg1	mice					787:790	HFD-fed mice	779:790	HFD-fed mice	779:790	We observed that SA supplementation significantly decreased body weight gain, cholesterol levels, and fat weight, while improving glucose tolerance in HFD-fed mice.
34444972	4	50	theme	dietary	528:534	arg1	fiber					536:540	a seaweed-derived dietary fiber	510:540	a seaweed-derived dietary fiber that suppresses high-fat diet (HFD)-induced MetS via an effect on the gut microbiota	510:625	Sodium alginate (SA) is a seaweed-derived dietary fiber that suppresses high-fat diet (HFD)-induced MetS via an effect on the gut microbiota.
34444972	4	50	theme	dietary	528:534	arg1	alginate					493:500	Sodium alginate	486:500	Sodium alginate (SA)	486:505	Sodium alginate (SA) is a seaweed-derived dietary fiber that suppresses high-fat diet (HFD)-induced MetS via an effect on the gut microbiota.
34444972	3	51	theme	dietary	414:420	arg1	fiber					422:426	dietary fiber	414:426	dietary fiber	414:426	This study aimed to assess the ability of dietary fiber to control MetS by modulating gut microbiota composition.
34444972	8	52	theme	MetS	1064:1067	arg1	development					1069:1079	MetS development	1064:1079	MetS development	1064:1079	Mechanistically, SA decreased the number of colonic inflammatory monocytes, which promote MetS development, in a gut microbiota-dependent manner.
34444972	7	53	from	effects	951:957	arg1	MetS					968:971	MetS	968:971	MetS	968:971	Antibiotic treatment completely abolished the suppressive effects of SA on MetS.
34444972	5	54	theme	body	688:691	arg1	gain					700:703	body weight gain	688:703	body weight gain	688:703	We observed that SA supplementation significantly decreased body weight gain, cholesterol levels, and fat weight, while improving glucose tolerance in HFD-fed mice.
34444972	10	55	theme	HFD-induced	1350:1360	arg1	MetS					1362:1365	HFD-induced MetS	1350:1365	HFD-induced MetS	1350:1365	The present study revealed a novel food function of SA in preventing HFD-induced MetS through its action on gut microbiota.
34444972	5	56	theme	weight	693:698	arg1	gain					700:703	body weight gain	688:703	body weight gain	688:703	We observed that SA supplementation significantly decreased body weight gain, cholesterol levels, and fat weight, while improving glucose tolerance in HFD-fed mice.
34444972	6	57	theme	microbiota	812:821	arg1	composition					823:833	the gut microbiota composition	804:833	the gut microbiota composition	804:833	SA changed the gut microbiota composition and significantly increased the abundance of Bacteroides.
34444972	0	58	theme	Colonic	66:72	arg1	Monocytes					87:95	Colonic Inflammatory Monocytes	66:95	Colonic Inflammatory Monocytes	66:95	Seaweed Dietary Fiber Sodium Alginate Suppresses the Migration of Colonic Inflammatory Monocytes and Diet-Induced Metabolic Syndrome via the Gut Microbiota.
34444972	6	59	theme	gut	808:810	arg1	composition					823:833	the gut microbiota composition	804:833	the gut microbiota composition	804:833	SA changed the gut microbiota composition and significantly increased the abundance of Bacteroides.
34444972	10	60	theme	gut	1389:1391	arg1	microbiota					1393:1402	gut microbiota	1389:1402	gut microbiota	1389:1402	The present study revealed a novel food function of SA in preventing HFD-induced MetS through its action on gut microbiota.
34444972	5	61	from	tolerance	766:774	arg1	mice					787:790	HFD-fed mice	779:790	HFD-fed mice	779:790	We observed that SA supplementation significantly decreased body weight gain, cholesterol levels, and fat weight, while improving glucose tolerance in HFD-fed mice.
34444972	1	62	theme	Metabolic	157:165	arg1	MetS					177:180	MetS	177:180	MetS	177:180	Metabolic syndrome (MetS) is a multifactorial chronic metabolic disorder that affects approximately one billion people worldwide.
34444972	1	62	theme	Metabolic	157:165	arg1	syndrome					167:174	Metabolic syndrome	157:174	Metabolic syndrome (MetS)	157:181	Metabolic syndrome (MetS) is a multifactorial chronic metabolic disorder that affects approximately one billion people worldwide.
34444972	1	62	theme	Metabolic	157:165	arg1	disorder					221:228	a multifactorial chronic metabolic disorder	186:228	a multifactorial chronic metabolic disorder that affects approximately one billion people worldwide	186:284	Metabolic syndrome (MetS) is a multifactorial chronic metabolic disorder that affects approximately one billion people worldwide.
34444972	8	63	theme	microbiota-dependent	1091:1110	arg1	manner					1112:1117	a gut microbiota-dependent manner	1085:1117	a gut microbiota-dependent manner	1085:1117	Mechanistically, SA decreased the number of colonic inflammatory monocytes, which promote MetS development, in a gut microbiota-dependent manner.
32496134	2	0	dep	Considering	248:258	arg1	leaves					366:371	leaves	366:371	Considering the anti-inflammatory and antinociceptive activities of the hydrometanolic fraction (FHMeOH) of A. nutans leaves previously reported	248:391	Considering the anti-inflammatory and antinociceptive activities of the hydrometanolic fraction (FHMeOH) of A. nutans leaves previously reported, the present study aimed to evaluate in vivo anti-inflammatory and antinociceptive activities of a subfraction obtained from FHMeOH, the butanolic fraction (FBuOHf).
32496134	2	1	theme	antinociceptive	460:474	arg1	activities					476:485	in vivo anti-inflammatory and antinociceptive activities	430:485	in vivo anti-inflammatory and antinociceptive activities of a subfraction obtained from FHMeOH, the butanolic fraction (FBuOHf)	430:556	Considering the anti-inflammatory and antinociceptive activities of the hydrometanolic fraction (FHMeOH) of A. nutans leaves previously reported, the present study aimed to evaluate in vivo anti-inflammatory and antinociceptive activities of a subfraction obtained from FHMeOH, the butanolic fraction (FBuOHf).
32496134	6	2	dep	fraction	945:952	arg1	200 mg · kg-1					955:967	200 mg · kg-1	955:967	200 mg · kg-1	955:967	In the formalin test, this fraction (200 mg · kg-1, i.p.) reduced licking time only in the inflammatory phase.
32496134	7	3	theme	cinnamic	1064:1071	arg1	acid					1254:1257	chlorogenic acid	1242:1257	chlorogenic acid	1242:1257	The FBuOHf contents flavonoids and cinnamic acid derivatives, such as quercetin-3-O-galactoside, quercetin-3-O-glucoside, isorhamnetin-3-O-galactoside, quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside and chlorogenic acid, identified and quantified by LC-MS.
32496134	7	3	theme	cinnamic	1064:1071	arg1	quercetin-3-O-galactoside					1099:1123	quercetin-3-O-galactoside	1099:1123	quercetin-3-O-galactoside	1099:1123	The FBuOHf contents flavonoids and cinnamic acid derivatives, such as quercetin-3-O-galactoside, quercetin-3-O-glucoside, isorhamnetin-3-O-galactoside, quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside and chlorogenic acid, identified and quantified by LC-MS.
32496134	7	3	theme	cinnamic	1064:1071	arg1	quercetin-3-O-glucoside					1126:1148	quercetin-3-O-glucoside	1126:1148	quercetin-3-O-glucoside	1126:1148	The FBuOHf contents flavonoids and cinnamic acid derivatives, such as quercetin-3-O-galactoside, quercetin-3-O-glucoside, isorhamnetin-3-O-galactoside, quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside and chlorogenic acid, identified and quantified by LC-MS.
32496134	7	3	theme	cinnamic	1064:1071	arg1	derivatives					1078:1088	cinnamic acid derivatives	1064:1088	cinnamic acid derivatives	1064:1088	The FBuOHf contents flavonoids and cinnamic acid derivatives, such as quercetin-3-O-galactoside, quercetin-3-O-glucoside, isorhamnetin-3-O-galactoside, quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside and chlorogenic acid, identified and quantified by LC-MS.
32496134	7	3	theme	cinnamic	1064:1071	arg1	-galactopyranoside					1219:1236	quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside	1181:1236	quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside	1181:1236	The FBuOHf contents flavonoids and cinnamic acid derivatives, such as quercetin-3-O-galactoside, quercetin-3-O-glucoside, isorhamnetin-3-O-galactoside, quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside and chlorogenic acid, identified and quantified by LC-MS.
32496134	7	3	theme	cinnamic	1064:1071	arg1	isorhamnetin-3-O-galactoside					1151:1178	isorhamnetin-3-O-galactoside	1151:1178	isorhamnetin-3-O-galactoside	1151:1178	The FBuOHf contents flavonoids and cinnamic acid derivatives, such as quercetin-3-O-galactoside, quercetin-3-O-glucoside, isorhamnetin-3-O-galactoside, quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside and chlorogenic acid, identified and quantified by LC-MS.
32496134	2	4	theme	fraction	335:342	arg1	activities					302:311	the anti-inflammatory and antinociceptive activities	260:311	the anti-inflammatory and antinociceptive activities of the hydrometanolic fraction (FHMeOH) of A. nutans	260:364	Considering the anti-inflammatory and antinociceptive activities of the hydrometanolic fraction (FHMeOH) of A. nutans leaves previously reported, the present study aimed to evaluate in vivo anti-inflammatory and antinociceptive activities of a subfraction obtained from FHMeOH, the butanolic fraction (FBuOHf).
32496134	0	5	theme	nutans	104:109	arg1	fraction					85:92	the butanolic fraction	71:92	the butanolic fraction of Annona nutans	71:109	Chemical composition, anti-inflammatory and antinociceptive effects of the butanolic fraction of Annona nutans (Annonaceae) leaves.
32496134	7	6	theme	contents	1040:1047	arg1	flavonoids					1049:1058	The FBuOHf contents flavonoids	1029:1058	The FBuOHf contents flavonoids	1029:1058	The FBuOHf contents flavonoids and cinnamic acid derivatives, such as quercetin-3-O-galactoside, quercetin-3-O-glucoside, isorhamnetin-3-O-galactoside, quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside and chlorogenic acid, identified and quantified by LC-MS.
32496134	7	6	theme	contents	1040:1047	arg1	acid					1254:1257	chlorogenic acid	1242:1257	chlorogenic acid	1242:1257	The FBuOHf contents flavonoids and cinnamic acid derivatives, such as quercetin-3-O-galactoside, quercetin-3-O-glucoside, isorhamnetin-3-O-galactoside, quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside and chlorogenic acid, identified and quantified by LC-MS.
32496134	7	6	theme	contents	1040:1047	arg1	quercetin-3-O-galactoside					1099:1123	quercetin-3-O-galactoside	1099:1123	quercetin-3-O-galactoside	1099:1123	The FBuOHf contents flavonoids and cinnamic acid derivatives, such as quercetin-3-O-galactoside, quercetin-3-O-glucoside, isorhamnetin-3-O-galactoside, quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside and chlorogenic acid, identified and quantified by LC-MS.
32496134	7	6	theme	contents	1040:1047	arg1	quercetin-3-O-glucoside					1126:1148	quercetin-3-O-glucoside	1126:1148	quercetin-3-O-glucoside	1126:1148	The FBuOHf contents flavonoids and cinnamic acid derivatives, such as quercetin-3-O-galactoside, quercetin-3-O-glucoside, isorhamnetin-3-O-galactoside, quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside and chlorogenic acid, identified and quantified by LC-MS.
32496134	7	6	theme	contents	1040:1047	arg1	-galactopyranoside					1219:1236	quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside	1181:1236	quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside	1181:1236	The FBuOHf contents flavonoids and cinnamic acid derivatives, such as quercetin-3-O-galactoside, quercetin-3-O-glucoside, isorhamnetin-3-O-galactoside, quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside and chlorogenic acid, identified and quantified by LC-MS.
32496134	7	6	theme	contents	1040:1047	arg1	isorhamnetin-3-O-galactoside					1151:1178	isorhamnetin-3-O-galactoside	1151:1178	isorhamnetin-3-O-galactoside	1151:1178	The FBuOHf contents flavonoids and cinnamic acid derivatives, such as quercetin-3-O-galactoside, quercetin-3-O-glucoside, isorhamnetin-3-O-galactoside, quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside and chlorogenic acid, identified and quantified by LC-MS.
32496134	5	7	dep	FBuOHf	826:831	arg1	100					838:840	100	838:840	100	838:840	Regarding the antinociceptive activity, FBuOHf (50, 100, and 200 mg · kg-1, i.p.) inhibited acetic acid-induced abdominal writhing.
32496134	5	7	dep	FBuOHf	826:831	arg1	200 mg · kg-1					847:859	200 mg · kg-1	847:859	200 mg · kg-1	847:859	Regarding the antinociceptive activity, FBuOHf (50, 100, and 200 mg · kg-1, i.p.) inhibited acetic acid-induced abdominal writhing.
32496134	5	7	dep	FBuOHf	826:831	arg1	50					834:835	50	834:835	50	834:835	Regarding the antinociceptive activity, FBuOHf (50, 100, and 200 mg · kg-1, i.p.) inhibited acetic acid-induced abdominal writhing.
32496134	4	8	theme	polymorphonuclear	726:742	arg1	migration					760:768	polymorphonuclear (PMN) leukocyte migration	726:768	polymorphonuclear (PMN) leukocyte migration	726:768	Moreover, FBuOHf (100 mg · kg-1, i.p.) also suppressed polymorphonuclear (PMN) leukocyte migration in the footpad.
32496134	0	9	theme	Annona	97:102	arg1	nutans					104:109	Annona nutans	97:109	Annona nutans	97:109	Chemical composition, anti-inflammatory and antinociceptive effects of the butanolic fraction of Annona nutans (Annonaceae) leaves.
32496134	7	10	theme	1→2	1215:1217	arg1	-galactopyranoside					1219:1236	quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside	1181:1236	quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside	1181:1236	The FBuOHf contents flavonoids and cinnamic acid derivatives, such as quercetin-3-O-galactoside, quercetin-3-O-glucoside, isorhamnetin-3-O-galactoside, quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside and chlorogenic acid, identified and quantified by LC-MS.
32496134	8	11	theme	peripheral	1339:1348	arg1	activities					1366:1375	anti-inflammatory and peripheral antinociceptive activities	1317:1375	anti-inflammatory and peripheral antinociceptive activities	1317:1375	The FBuOHf possesses anti-inflammatory and peripheral antinociceptive activities.
32496134	6	12	theme	licking	984:990	arg1	time					992:995	licking time	984:995	licking time	984:995	In the formalin test, this fraction (200 mg · kg-1, i.p.) reduced licking time only in the inflammatory phase.
32496134	4	13	theme	PMN	745:747	arg1	migration					760:768	polymorphonuclear (PMN) leukocyte migration	726:768	polymorphonuclear (PMN) leukocyte migration	726:768	Moreover, FBuOHf (100 mg · kg-1, i.p.) also suppressed polymorphonuclear (PMN) leukocyte migration in the footpad.
32496134	3	14	dep	Intraperitoneal	559:573	arg1	i.p.					576:579	i.p.	576:579	i.p.	576:579	Intraperitoneal (i.p.) treatment with FBuOHf (50 and 100 mg · kg-1) inhibited paw edema induced by carrageenan.
32496134	2	15	theme	subfraction	492:502	arg1	activities					476:485	in vivo anti-inflammatory and antinociceptive activities	430:485	in vivo anti-inflammatory and antinociceptive activities of a subfraction obtained from FHMeOH, the butanolic fraction (FBuOHf)	430:556	Considering the anti-inflammatory and antinociceptive activities of the hydrometanolic fraction (FHMeOH) of A. nutans leaves previously reported, the present study aimed to evaluate in vivo anti-inflammatory and antinociceptive activities of a subfraction obtained from FHMeOH, the butanolic fraction (FBuOHf).
32496134	7	16	theme	FBuOHf	1033:1038	arg1	contents					1040:1047	The FBuOHf contents	1029:1047	The FBuOHf contents flavonoids	1029:1058	The FBuOHf contents flavonoids and cinnamic acid derivatives, such as quercetin-3-O-galactoside, quercetin-3-O-glucoside, isorhamnetin-3-O-galactoside, quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside and chlorogenic acid, identified and quantified by LC-MS.
32496134	5	17	theme	acetic	878:883	arg1	writhing					908:915	acetic acid-induced abdominal writhing	878:915	acetic acid-induced abdominal writhing	878:915	Regarding the antinociceptive activity, FBuOHf (50, 100, and 200 mg · kg-1, i.p.) inhibited acetic acid-induced abdominal writhing.
32496134	2	18	theme	nutans	359:364	arg1	FHMeOH					345:350	FHMeOH	345:350	FHMeOH	345:350	Considering the anti-inflammatory and antinociceptive activities of the hydrometanolic fraction (FHMeOH) of A. nutans leaves previously reported, the present study aimed to evaluate in vivo anti-inflammatory and antinociceptive activities of a subfraction obtained from FHMeOH, the butanolic fraction (FBuOHf).
32496134	2	18	theme	nutans	359:364	arg1	fraction					335:342	the hydrometanolic fraction	316:342	the hydrometanolic fraction (FHMeOH) of A. nutans	316:364	Considering the anti-inflammatory and antinociceptive activities of the hydrometanolic fraction (FHMeOH) of A. nutans leaves previously reported, the present study aimed to evaluate in vivo anti-inflammatory and antinociceptive activities of a subfraction obtained from FHMeOH, the butanolic fraction (FBuOHf).
32496134	0	19	theme	fraction	85:92	arg1	effects					60:66	anti-inflammatory and antinociceptive effects	22:66	anti-inflammatory and antinociceptive effects of the butanolic fraction of Annona nutans	22:109	Chemical composition, anti-inflammatory and antinociceptive effects of the butanolic fraction of Annona nutans (Annonaceae) leaves.
32496134	0	19	theme	fraction	85:92	arg1	composition					9:19	Chemical composition	0:19	Chemical composition	0:19	Chemical composition, anti-inflammatory and antinociceptive effects of the butanolic fraction of Annona nutans (Annonaceae) leaves.
32496134	6	20	theme	inflammatory	1009:1020	arg1	phase					1022:1026	the inflammatory phase	1005:1026	the inflammatory phase	1005:1026	In the formalin test, this fraction (200 mg · kg-1, i.p.) reduced licking time only in the inflammatory phase.
32496134	3	21	with	treatment	582:590	arg1	FBuOHf					597:602	FBuOHf	597:602	FBuOHf (50 and 100 mg · kg-1)	597:625	Intraperitoneal (i.p.) treatment with FBuOHf (50 and 100 mg · kg-1) inhibited paw edema induced by carrageenan.
32496134	2	22	theme	antinociceptive	286:300	arg1	activities					302:311	the anti-inflammatory and antinociceptive activities	260:311	the anti-inflammatory and antinociceptive activities of the hydrometanolic fraction (FHMeOH) of A. nutans	260:364	Considering the anti-inflammatory and antinociceptive activities of the hydrometanolic fraction (FHMeOH) of A. nutans leaves previously reported, the present study aimed to evaluate in vivo anti-inflammatory and antinociceptive activities of a subfraction obtained from FHMeOH, the butanolic fraction (FBuOHf).
32496134	0	23	theme	Chemical	0:7	arg1	effects					60:66	anti-inflammatory and antinociceptive effects	22:66	anti-inflammatory and antinociceptive effects of the butanolic fraction of Annona nutans	22:109	Chemical composition, anti-inflammatory and antinociceptive effects of the butanolic fraction of Annona nutans (Annonaceae) leaves.
32496134	0	23	theme	Chemical	0:7	arg1	composition					9:19	Chemical composition	0:19	Chemical composition	0:19	Chemical composition, anti-inflammatory and antinociceptive effects of the butanolic fraction of Annona nutans (Annonaceae) leaves.
32496134	0	23	theme	Chemical	0:7	arg1	Annonaceae					112:121	Annonaceae	112:121	Annonaceae	112:121	Chemical composition, anti-inflammatory and antinociceptive effects of the butanolic fraction of Annona nutans (Annonaceae) leaves.
32496134	1	24	theme	Brazilian	229:237	arg1	cerrado					239:245	the Brazilian cerrado	225:245	the Brazilian cerrado	225:245	The species Annona nutans (R. E. Fries) is a plant found in Bolivia, Paraguay, Argentina and the Brazilian cerrado.
32496134	5	25	theme	acid-induced	885:896	arg1	writhing					908:915	acetic acid-induced abdominal writhing	878:915	acetic acid-induced abdominal writhing	878:915	Regarding the antinociceptive activity, FBuOHf (50, 100, and 200 mg · kg-1, i.p.) inhibited acetic acid-induced abdominal writhing.
32496134	0	26	theme	anti-inflammatory	22:38	arg1	effects					60:66	anti-inflammatory and antinociceptive effects	22:66	anti-inflammatory and antinociceptive effects of the butanolic fraction of Annona nutans	22:109	Chemical composition, anti-inflammatory and antinociceptive effects of the butanolic fraction of Annona nutans (Annonaceae) leaves.
32496134	0	26	theme	anti-inflammatory	22:38	arg1	composition					9:19	Chemical composition	0:19	Chemical composition	0:19	Chemical composition, anti-inflammatory and antinociceptive effects of the butanolic fraction of Annona nutans (Annonaceae) leaves.
32496134	2	27	theme	anti-inflammatory	264:280	arg1	activities					302:311	the anti-inflammatory and antinociceptive activities	260:311	the anti-inflammatory and antinociceptive activities of the hydrometanolic fraction (FHMeOH) of A. nutans	260:364	Considering the anti-inflammatory and antinociceptive activities of the hydrometanolic fraction (FHMeOH) of A. nutans leaves previously reported, the present study aimed to evaluate in vivo anti-inflammatory and antinociceptive activities of a subfraction obtained from FHMeOH, the butanolic fraction (FBuOHf).
32496134	2	28	theme	present	398:404	arg1	study					406:410	the present study	394:410	the present study	394:410	Considering the anti-inflammatory and antinociceptive activities of the hydrometanolic fraction (FHMeOH) of A. nutans leaves previously reported, the present study aimed to evaluate in vivo anti-inflammatory and antinociceptive activities of a subfraction obtained from FHMeOH, the butanolic fraction (FBuOHf).
32496134	2	29	theme	butanolic	530:538	arg1	FBuOHf					550:555	FBuOHf	550:555	FBuOHf	550:555	Considering the anti-inflammatory and antinociceptive activities of the hydrometanolic fraction (FHMeOH) of A. nutans leaves previously reported, the present study aimed to evaluate in vivo anti-inflammatory and antinociceptive activities of a subfraction obtained from FHMeOH, the butanolic fraction (FBuOHf).
32496134	2	29	theme	butanolic	530:538	arg1	fraction					540:547	the butanolic fraction	526:547	the butanolic fraction (FBuOHf)	526:556	Considering the anti-inflammatory and antinociceptive activities of the hydrometanolic fraction (FHMeOH) of A. nutans leaves previously reported, the present study aimed to evaluate in vivo anti-inflammatory and antinociceptive activities of a subfraction obtained from FHMeOH, the butanolic fraction (FBuOHf).
32496134	2	29	theme	butanolic	530:538	arg1	FHMeOH					518:523	FHMeOH	518:523	FHMeOH	518:523	Considering the anti-inflammatory and antinociceptive activities of the hydrometanolic fraction (FHMeOH) of A. nutans leaves previously reported, the present study aimed to evaluate in vivo anti-inflammatory and antinociceptive activities of a subfraction obtained from FHMeOH, the butanolic fraction (FBuOHf).
32496134	7	30	theme	chlorogenic	1242:1252	arg1	acid					1254:1257	chlorogenic acid	1242:1257	chlorogenic acid	1242:1257	The FBuOHf contents flavonoids and cinnamic acid derivatives, such as quercetin-3-O-galactoside, quercetin-3-O-glucoside, isorhamnetin-3-O-galactoside, quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside and chlorogenic acid, identified and quantified by LC-MS.
32496134	5	31	theme	antinociceptive	800:814	arg1	activity					816:823	the antinociceptive activity	796:823	the antinociceptive activity	796:823	Regarding the antinociceptive activity, FBuOHf (50, 100, and 200 mg · kg-1, i.p.) inhibited acetic acid-induced abdominal writhing.
32496134	0	32	theme	antinociceptive	44:58	arg1	effects					60:66	anti-inflammatory and antinociceptive effects	22:66	anti-inflammatory and antinociceptive effects of the butanolic fraction of Annona nutans	22:109	Chemical composition, anti-inflammatory and antinociceptive effects of the butanolic fraction of Annona nutans (Annonaceae) leaves.
32496134	0	32	theme	antinociceptive	44:58	arg1	composition					9:19	Chemical composition	0:19	Chemical composition	0:19	Chemical composition, anti-inflammatory and antinociceptive effects of the butanolic fraction of Annona nutans (Annonaceae) leaves.
32496134	8	33	contain	possesses	1307:1315	arg1	FBuOHf					1300:1305	The FBuOHf	1296:1305	The FBuOHf	1296:1305	The FBuOHf possesses anti-inflammatory and peripheral antinociceptive activities.
32496134	8	33	contain	possesses	1307:1315	arg2	activities					1366:1375	anti-inflammatory and peripheral antinociceptive activities	1317:1375	anti-inflammatory and peripheral antinociceptive activities	1317:1375	The FBuOHf possesses anti-inflammatory and peripheral antinociceptive activities.
32496134	3	34	dep	FBuOHf	597:602	arg1	100 mg · kg-1					612:624	100 mg · kg-1	612:624	100 mg · kg-1	612:624	Intraperitoneal (i.p.) treatment with FBuOHf (50 and 100 mg · kg-1) inhibited paw edema induced by carrageenan.
32496134	3	34	dep	FBuOHf	597:602	arg1	50					605:606	50	605:606	50	605:606	Intraperitoneal (i.p.) treatment with FBuOHf (50 and 100 mg · kg-1) inhibited paw edema induced by carrageenan.
32496134	2	35	theme	anti-inflammatory	438:454	arg1	activities					476:485	in vivo anti-inflammatory and antinociceptive activities	430:485	in vivo anti-inflammatory and antinociceptive activities of a subfraction obtained from FHMeOH, the butanolic fraction (FBuOHf)	430:556	Considering the anti-inflammatory and antinociceptive activities of the hydrometanolic fraction (FHMeOH) of A. nutans leaves previously reported, the present study aimed to evaluate in vivo anti-inflammatory and antinociceptive activities of a subfraction obtained from FHMeOH, the butanolic fraction (FBuOHf).
32496134	2	36	theme	in vivo	430:436	arg1	activities					476:485	in vivo anti-inflammatory and antinociceptive activities	430:485	in vivo anti-inflammatory and antinociceptive activities of a subfraction obtained from FHMeOH, the butanolic fraction (FBuOHf)	430:556	Considering the anti-inflammatory and antinociceptive activities of the hydrometanolic fraction (FHMeOH) of A. nutans leaves previously reported, the present study aimed to evaluate in vivo anti-inflammatory and antinociceptive activities of a subfraction obtained from FHMeOH, the butanolic fraction (FBuOHf).
32496134	3	37	theme	Intraperitoneal	559:573	arg1	treatment					582:590	Intraperitoneal (i.p.) treatment	559:590	Intraperitoneal (i.p.) treatment with FBuOHf (50 and 100 mg · kg-1)	559:625	Intraperitoneal (i.p.) treatment with FBuOHf (50 and 100 mg · kg-1) inhibited paw edema induced by carrageenan.
32496134	2	38	theme	hydrometanolic	320:333	arg1	FHMeOH					345:350	FHMeOH	345:350	FHMeOH	345:350	Considering the anti-inflammatory and antinociceptive activities of the hydrometanolic fraction (FHMeOH) of A. nutans leaves previously reported, the present study aimed to evaluate in vivo anti-inflammatory and antinociceptive activities of a subfraction obtained from FHMeOH, the butanolic fraction (FBuOHf).
32496134	2	38	theme	hydrometanolic	320:333	arg1	fraction					335:342	the hydrometanolic fraction	316:342	the hydrometanolic fraction (FHMeOH) of A. nutans	316:364	Considering the anti-inflammatory and antinociceptive activities of the hydrometanolic fraction (FHMeOH) of A. nutans leaves previously reported, the present study aimed to evaluate in vivo anti-inflammatory and antinociceptive activities of a subfraction obtained from FHMeOH, the butanolic fraction (FBuOHf).
32496134	5	39	theme	abdominal	898:906	arg1	writhing					908:915	acetic acid-induced abdominal writhing	878:915	acetic acid-induced abdominal writhing	878:915	Regarding the antinociceptive activity, FBuOHf (50, 100, and 200 mg · kg-1, i.p.) inhibited acetic acid-induced abdominal writhing.
32496134	4	40	theme	leukocyte	750:758	arg1	migration					760:768	polymorphonuclear (PMN) leukocyte migration	726:768	polymorphonuclear (PMN) leukocyte migration	726:768	Moreover, FBuOHf (100 mg · kg-1, i.p.) also suppressed polymorphonuclear (PMN) leukocyte migration in the footpad.
32496134	4	41	dep	FBuOHf	681:686	arg1	100 mg · kg-1					689:701	100 mg · kg-1	689:701	100 mg · kg-1	689:701	Moreover, FBuOHf (100 mg · kg-1, i.p.) also suppressed polymorphonuclear (PMN) leukocyte migration in the footpad.
32496134	1	42	located	found	183:187	arg1	Argentina					211:219	Argentina	211:219	Argentina	211:219	The species Annona nutans (R. E. Fries) is a plant found in Bolivia, Paraguay, Argentina and the Brazilian cerrado.
32496134	1	42	located	found	183:187	arg2	plant					177:181	a plant	175:181	a plant found in Bolivia, Paraguay, Argentina and the Brazilian cerrado	175:245	The species Annona nutans (R. E. Fries) is a plant found in Bolivia, Paraguay, Argentina and the Brazilian cerrado.
32496134	1	42	located	found	183:187	arg1	cerrado					239:245	the Brazilian cerrado	225:245	the Brazilian cerrado	225:245	The species Annona nutans (R. E. Fries) is a plant found in Bolivia, Paraguay, Argentina and the Brazilian cerrado.
32496134	1	42	located	found	183:187	arg2	nutans					151:156	The species Annona nutans	132:156	The species Annona nutans (R. E. Fries)	132:170	The species Annona nutans (R. E. Fries) is a plant found in Bolivia, Paraguay, Argentina and the Brazilian cerrado.
32496134	1	42	located	found	183:187	arg1	Bolivia					192:198	Bolivia	192:198	Bolivia	192:198	The species Annona nutans (R. E. Fries) is a plant found in Bolivia, Paraguay, Argentina and the Brazilian cerrado.
32496134	8	43	theme	anti-inflammatory	1317:1333	arg1	activities					1366:1375	anti-inflammatory and peripheral antinociceptive activities	1317:1375	anti-inflammatory and peripheral antinociceptive activities	1317:1375	The FBuOHf possesses anti-inflammatory and peripheral antinociceptive activities.
32496134	8	44	theme	antinociceptive	1350:1364	arg1	activities					1366:1375	anti-inflammatory and peripheral antinociceptive activities	1317:1375	anti-inflammatory and peripheral antinociceptive activities	1317:1375	The FBuOHf possesses anti-inflammatory and peripheral antinociceptive activities.
32496134	0	45	theme	butanolic	75:83	arg1	fraction					85:92	the butanolic fraction	71:92	the butanolic fraction of Annona nutans	71:109	Chemical composition, anti-inflammatory and antinociceptive effects of the butanolic fraction of Annona nutans (Annonaceae) leaves.
32496134	3	46	theme	paw	637:639	arg1	edema					641:645	paw edema	637:645	paw edema induced by carrageenan	637:668	Intraperitoneal (i.p.) treatment with FBuOHf (50 and 100 mg · kg-1) inhibited paw edema induced by carrageenan.
32496134	1	47	theme	species	136:142	arg1	Fries					165:169	Fries	165:169	Fries	165:169	The species Annona nutans (R. E. Fries) is a plant found in Bolivia, Paraguay, Argentina and the Brazilian cerrado.
32496134	1	47	theme	species	136:142	arg1	plant					177:181	a plant	175:181	a plant found in Bolivia, Paraguay, Argentina and the Brazilian cerrado	175:245	The species Annona nutans (R. E. Fries) is a plant found in Bolivia, Paraguay, Argentina and the Brazilian cerrado.
32496134	1	47	theme	species	136:142	arg1	nutans					151:156	The species Annona nutans	132:156	The species Annona nutans (R. E. Fries)	132:170	The species Annona nutans (R. E. Fries) is a plant found in Bolivia, Paraguay, Argentina and the Brazilian cerrado.
32496134	7	48	theme	acid	1073:1076	arg1	acid					1254:1257	chlorogenic acid	1242:1257	chlorogenic acid	1242:1257	The FBuOHf contents flavonoids and cinnamic acid derivatives, such as quercetin-3-O-galactoside, quercetin-3-O-glucoside, isorhamnetin-3-O-galactoside, quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside and chlorogenic acid, identified and quantified by LC-MS.
32496134	7	48	theme	acid	1073:1076	arg1	quercetin-3-O-galactoside					1099:1123	quercetin-3-O-galactoside	1099:1123	quercetin-3-O-galactoside	1099:1123	The FBuOHf contents flavonoids and cinnamic acid derivatives, such as quercetin-3-O-galactoside, quercetin-3-O-glucoside, isorhamnetin-3-O-galactoside, quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside and chlorogenic acid, identified and quantified by LC-MS.
32496134	7	48	theme	acid	1073:1076	arg1	quercetin-3-O-glucoside					1126:1148	quercetin-3-O-glucoside	1126:1148	quercetin-3-O-glucoside	1126:1148	The FBuOHf contents flavonoids and cinnamic acid derivatives, such as quercetin-3-O-galactoside, quercetin-3-O-glucoside, isorhamnetin-3-O-galactoside, quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside and chlorogenic acid, identified and quantified by LC-MS.
32496134	7	48	theme	acid	1073:1076	arg1	derivatives					1078:1088	cinnamic acid derivatives	1064:1088	cinnamic acid derivatives	1064:1088	The FBuOHf contents flavonoids and cinnamic acid derivatives, such as quercetin-3-O-galactoside, quercetin-3-O-glucoside, isorhamnetin-3-O-galactoside, quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside and chlorogenic acid, identified and quantified by LC-MS.
32496134	7	48	theme	acid	1073:1076	arg1	-galactopyranoside					1219:1236	quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside	1181:1236	quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside	1181:1236	The FBuOHf contents flavonoids and cinnamic acid derivatives, such as quercetin-3-O-galactoside, quercetin-3-O-glucoside, isorhamnetin-3-O-galactoside, quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside and chlorogenic acid, identified and quantified by LC-MS.
32496134	7	48	theme	acid	1073:1076	arg1	isorhamnetin-3-O-galactoside					1151:1178	isorhamnetin-3-O-galactoside	1151:1178	isorhamnetin-3-O-galactoside	1151:1178	The FBuOHf contents flavonoids and cinnamic acid derivatives, such as quercetin-3-O-galactoside, quercetin-3-O-glucoside, isorhamnetin-3-O-galactoside, quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside and chlorogenic acid, identified and quantified by LC-MS.
32496134	6	49	theme	formalin	925:932	arg1	test					934:937	the formalin test	921:937	the formalin test	921:937	In the formalin test, this fraction (200 mg · kg-1, i.p.) reduced licking time only in the inflammatory phase.
32496134	1	50	theme	Annona	144:149	arg1	Fries					165:169	Fries	165:169	Fries	165:169	The species Annona nutans (R. E. Fries) is a plant found in Bolivia, Paraguay, Argentina and the Brazilian cerrado.
32496134	1	50	theme	Annona	144:149	arg1	plant					177:181	a plant	175:181	a plant found in Bolivia, Paraguay, Argentina and the Brazilian cerrado	175:245	The species Annona nutans (R. E. Fries) is a plant found in Bolivia, Paraguay, Argentina and the Brazilian cerrado.
32496134	1	50	theme	Annona	144:149	arg1	nutans					151:156	The species Annona nutans	132:156	The species Annona nutans (R. E. Fries)	132:170	The species Annona nutans (R. E. Fries) is a plant found in Bolivia, Paraguay, Argentina and the Brazilian cerrado.
32496134	7	51	theme	quercetin-3-O-β-D-apio-furanosyl-	1181:1213	arg1	-galactopyranoside					1219:1236	quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside	1181:1236	quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside	1181:1236	The FBuOHf contents flavonoids and cinnamic acid derivatives, such as quercetin-3-O-galactoside, quercetin-3-O-glucoside, isorhamnetin-3-O-galactoside, quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside and chlorogenic acid, identified and quantified by LC-MS.
34958956	3	0	theme	resting	777:783	arg1	rates					813:817	resting and post-prandial metabolic rates	777:817	resting and post-prandial metabolic rates at 6 and 12 months of age	777:843	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	1	theme	rates	813:817	arg1	11 months					759:767	11 months	759:767	11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition	759:901	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	4	2	dep	8 ± 2 days	1197:1206	arg1	P < 0.01					1225:1232	P < 0.01	1225:1232	P < 0.01	1225:1232	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	2	3	theme	%	513:513	arg1	starch					497:502	starch	497:502	starch (HS; 27.7% DMB)	497:518	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	2	3	theme	%	513:513	arg1	DMB					515:517	HS; 27.7% DMB	505:517	HS; 27.7% DMB	505:517	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	3	4	theme	age	841:843	arg1	12 months					828:836	12 months	828:836	12 months of age	828:843	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	5	theme	hatchlings	611:620	arg1	total					599:603	A total	597:603	A total of 20 hatchlings (10 per diet)	597:634	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	4	6	dep	3 ± 1 days	1139:1148	arg1	P < 0.01					1166:1173	P < 0.01	1166:1173	P < 0.01	1166:1173	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	0	7	theme	red-footed	97:106	arg1	hatchlings					117:126	red-footed tortoise hatchlings	97:126	red-footed tortoise hatchlings (Chelonoidis carbonaria)	97:151	Starch and fiber intake effects on energy metabolism, growth, and carapacial scute pyramiding of red-footed tortoise hatchlings (Chelonoidis carbonaria).
34958956	0	7	theme	red-footed	97:106	arg1	carbonaria					141:150	carbonaria	141:150	carbonaria	141:150	Starch and fiber intake effects on energy metabolism, growth, and carapacial scute pyramiding of red-footed tortoise hatchlings (Chelonoidis carbonaria).
34958956	2	8	theme	Juvenile	290:297	arg1	tortoises					310:318	Juvenile red-footed tortoises	290:318	Juvenile red-footed tortoises (Chelonoidis carbonaria)	290:343	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	1	9	theme	excessive	193:201	arg1	growth					203:208	excessive growth	193:208	excessive growth	193:208	Tortoise husbandry includes reports of excessive growth and carapace pyramiding, although triggers still remain to be fully elucidated.
34958956	4	10	theme	retention	1180:1188	arg1	times					1190:1194	retention times	1180:1194	retention times	1180:1194	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	0	11	theme	hatchlings	117:126	arg1	pyramiding					83:92	carapacial scute pyramiding	66:92	carapacial scute pyramiding of red-footed tortoise hatchlings (Chelonoidis carbonaria)	66:151	Starch and fiber intake effects on energy metabolism, growth, and carapacial scute pyramiding of red-footed tortoise hatchlings (Chelonoidis carbonaria).
34958956	0	11	theme	hatchlings	117:126	arg1	growth					54:59	growth	54:59	growth	54:59	Starch and fiber intake effects on energy metabolism, growth, and carapacial scute pyramiding of red-footed tortoise hatchlings (Chelonoidis carbonaria).
34958956	0	11	theme	hatchlings	117:126	arg1	metabolism					42:51	energy metabolism	35:51	energy metabolism	35:51	Starch and fiber intake effects on energy metabolism, growth, and carapacial scute pyramiding of red-footed tortoise hatchlings (Chelonoidis carbonaria).
34958956	6	12	theme	pyramiding	1358:1367	arg1	degree					1369:1374	pyramiding degree	1358:1374	pyramiding degree	1358:1374	Rest and post-prandial metabolic rates, and pyramiding degree were not affected by diets.
34958956	4	13	theme	energy	968:973	arg1	intake					947:952	higher mass-specific intake	926:952	higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE	926:1275	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	7	14	theme	carapace	1490:1497	arg1	rates					1512:1516	higher carapace width growth rates	1483:1516	higher carapace width growth rates	1483:1516	At 13 months, the animals from HS presented wider plastrons and carapaces, and higher carapace width growth rates.
34958956	2	15	theme	nutrient	561:568	arg1	digestibility					570:582	nutrient digestibility	561:582	nutrient digestibility	561:582	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	2	16	from	effects	531:537	arg1	metabolism					549:558	energy metabolism	542:558	energy metabolism	542:558	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	2	16	from	effects	531:537	arg1	digestibility					570:582	nutrient digestibility	561:582	nutrient digestibility	561:582	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	2	16	from	effects	531:537	arg1	growth					589:594	growth	589:594	growth	589:594	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	4	17	theme	Da	1247:1248	arg1	intake					947:952	higher mass-specific intake	926:952	higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE	926:1275	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	7	18	dep	wider	1448:1452	arg1	carapaces					1468:1476	carapaces	1468:1476	carapaces	1468:1476	At 13 months, the animals from HS presented wider plastrons and carapaces, and higher carapace width growth rates.
34958956	7	18	dep	wider	1448:1452	arg1	plastrons					1454:1462	plastrons	1454:1462	plastrons	1454:1462	At 13 months, the animals from HS presented wider plastrons and carapaces, and higher carapace width growth rates.
34958956	6	19	theme	metabolic	1337:1345	arg1	rates					1347:1351	post-prandial metabolic rates	1323:1351	post-prandial metabolic rates	1323:1351	Rest and post-prandial metabolic rates, and pyramiding degree were not affected by diets.
34958956	4	20	theme	mass-specific	933:945	arg1	intake					947:952	higher mass-specific intake	926:952	higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE	926:1275	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	21	theme	DM	1253:1254	arg1	6.1 ± 1.8 g kg-1 day-1					1060:1081	6.1 ± 1.8 g kg-1 day-1	1060:1081	6.1 ± 1.8 g kg-1 day-1	1060:1081	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	21	theme	DM	1253:1254	arg1	8 ± 2 days					1197:1206	8 ± 2 days	1197:1206	8 ± 2 days	1197:1206	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	21	theme	DM	1253:1254	arg1	energy					968:973	digestible energy	957:973	digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05)	957:1042	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	21	theme	DM	1253:1254	arg1	5.0 ± 1.8 g kg-1 day-1					1087:1108	5.0 ± 1.8 g kg-1 day-1	1087:1108	5.0 ± 1.8 g kg-1 day-1	1087:1108	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	21	theme	DM	1253:1254	arg1	10 ± 2 days					1212:1222	10 ± 2 days	1212:1222	10 ± 2 days	1212:1222	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	21	theme	DM	1253:1254	arg1	transit					1130:1136	shorter transit	1122:1136	shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01)	1122:1174	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	21	theme	DM	1253:1254	arg1	113.9 ± 32.1 kJ kg-1 day-1					976:1001	113.9 ± 32.1 kJ kg-1 day-1	976:1001	113.9 ± 32.1 kJ kg-1 day-1	976:1001	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	21	theme	DM	1253:1254	arg1	3 ± 1 days					1139:1148	3 ± 1 days	1139:1148	3 ± 1 days	1139:1148	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	21	theme	DM	1253:1254	arg1	Da					1247:1248	higher Da	1240:1248	higher Da of DM, starch, NDF, and GE	1240:1275	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	21	theme	DM	1253:1254	arg1	DM					1056:1057	digestible DM	1045:1057	digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01)	1045:1119	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	21	theme	DM	1253:1254	arg1	times					1190:1194	retention times	1180:1194	retention times	1180:1194	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	21	theme	DM	1253:1254	arg1	99.6 ± 35.3 kJ kg-1 day-1					1007:1031	99.6 ± 35.3 kJ kg-1 day-1	1007:1031	99.6 ± 35.3 kJ kg-1 day-1	1007:1031	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	21	theme	DM	1253:1254	arg1	4 ± 1 days					1154:1163	4 ± 1 days	1154:1163	4 ± 1 days	1154:1163	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	3	22	theme	gross	717:721	arg1	GE					731:732	GE	731:732	GE	731:732	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	22	theme	gross	717:721	arg1	energy					723:728	gross energy	717:728	gross energy (GE)	717:733	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	4	23	dep	6.1 ± 1.8 g kg-1 day-1	1060:1081	arg1	P < 0.01					1111:1118	P < 0.01	1111:1118	P < 0.01	1111:1118	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	2	24	theme	detergent	436:444	arg1	fiber					446:450	neutral detergent fiber	428:450	14.2% crude fiber; 39.2% neutral detergent fiber	403:450	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	3	25	theme	digestibility	668:680	arg1	times					744:748	passage times	736:748	passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition	736:901	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	25	theme	digestibility	668:680	arg1	Da					696:697	Da	696:697	Da	696:697	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	25	theme	digestibility	668:680	arg1	coefficients					682:693	apparent digestibility coefficients	659:693	apparent digestibility coefficients (Da) of nutrients and gross energy (GE)	659:733	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	26	dep	hatchlings	611:620	arg1	10					623:624	10	623:624	10	623:624	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	8	27	theme	lower	1550:1554	arg1	%					1606:1606	2.15 ± 0.19%	1595:1606	2.15 ± 0.19%	1595:1606	In addition, these animals had lower body mineral content (1.88 ± 0.15% vs. 2.15 ± 0.19%; P < 0.01) and bone density (0.13 ± 0.01 g mm-2 vs. 0.15 ± 0.02 g mm-2; P < 0.02).
34958956	8	27	theme	lower	1550:1554	arg1	content					1569:1575	lower body mineral content	1550:1575	lower body mineral content (1.88 ± 0.15% vs. 2.15 ± 0.19%; P < 0.01)	1550:1617	In addition, these animals had lower body mineral content (1.88 ± 0.15% vs. 2.15 ± 0.19%; P < 0.01) and bone density (0.13 ± 0.01 g mm-2 vs. 0.15 ± 0.02 g mm-2; P < 0.02).
34958956	8	27	theme	lower	1550:1554	arg1	%					1589:1589	1.88 ± 0.15%	1578:1589	1.88 ± 0.15%	1578:1589	In addition, these animals had lower body mineral content (1.88 ± 0.15% vs. 2.15 ± 0.19%; P < 0.01) and bone density (0.13 ± 0.01 g mm-2 vs. 0.15 ± 0.02 g mm-2; P < 0.02).
34958956	3	28	theme	nutrients	703:711	arg1	times					744:748	passage times	736:748	passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition	736:901	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	28	theme	nutrients	703:711	arg1	Da					696:697	Da	696:697	Da	696:697	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	28	theme	nutrients	703:711	arg1	coefficients					682:693	apparent digestibility coefficients	659:693	apparent digestibility coefficients (Da) of nutrients and gross energy (GE)	659:733	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	2	29	theme	matter	462:467	arg1	basis					469:473	dry matter basis	458:473	dry matter basis	458:473	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	0	30	theme	carapacial	66:75	arg1	pyramiding					83:92	carapacial scute pyramiding	66:92	carapacial scute pyramiding of red-footed tortoise hatchlings (Chelonoidis carbonaria)	66:151	Starch and fiber intake effects on energy metabolism, growth, and carapacial scute pyramiding of red-footed tortoise hatchlings (Chelonoidis carbonaria).
34958956	3	31	theme	growth	846:851	arg1	rates					853:857	growth rates	846:857	growth rates	846:857	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	8	32	theme	mineral	1561:1567	arg1	%					1606:1606	2.15 ± 0.19%	1595:1606	2.15 ± 0.19%	1595:1606	In addition, these animals had lower body mineral content (1.88 ± 0.15% vs. 2.15 ± 0.19%; P < 0.01) and bone density (0.13 ± 0.01 g mm-2 vs. 0.15 ± 0.02 g mm-2; P < 0.02).
34958956	8	32	theme	mineral	1561:1567	arg1	content					1569:1575	lower body mineral content	1550:1575	lower body mineral content (1.88 ± 0.15% vs. 2.15 ± 0.19%; P < 0.01)	1550:1617	In addition, these animals had lower body mineral content (1.88 ± 0.15% vs. 2.15 ± 0.19%; P < 0.01) and bone density (0.13 ± 0.01 g mm-2 vs. 0.15 ± 0.02 g mm-2; P < 0.02).
34958956	8	32	theme	mineral	1561:1567	arg1	%					1589:1589	1.88 ± 0.15%	1578:1589	1.88 ± 0.15%	1578:1589	In addition, these animals had lower body mineral content (1.88 ± 0.15% vs. 2.15 ± 0.19%; P < 0.01) and bone density (0.13 ± 0.01 g mm-2 vs. 0.15 ± 0.02 g mm-2; P < 0.02).
34958956	4	33	theme	DM	1056:1057	arg1	intake					947:952	higher mass-specific intake	926:952	higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE	926:1275	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	3	34	theme	estimated	876:884	arg1	composition					891:901	estimated body composition	876:901	estimated body composition	876:901	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	2	35	theme	crude	409:413	arg1	fiber					415:419	crude fiber	409:419	14.2% crude fiber; 39.2% neutral detergent fiber	403:450	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	0	36	from	effects	24:30	arg1	pyramiding					83:92	carapacial scute pyramiding	66:92	carapacial scute pyramiding of red-footed tortoise hatchlings (Chelonoidis carbonaria)	66:151	Starch and fiber intake effects on energy metabolism, growth, and carapacial scute pyramiding of red-footed tortoise hatchlings (Chelonoidis carbonaria).
34958956	0	36	from	effects	24:30	arg1	growth					54:59	growth	54:59	growth	54:59	Starch and fiber intake effects on energy metabolism, growth, and carapacial scute pyramiding of red-footed tortoise hatchlings (Chelonoidis carbonaria).
34958956	0	36	from	effects	24:30	arg1	metabolism					42:51	energy metabolism	35:51	energy metabolism	35:51	Starch and fiber intake effects on energy metabolism, growth, and carapacial scute pyramiding of red-footed tortoise hatchlings (Chelonoidis carbonaria).
34958956	9	37	theme	lower	1777:1781	arg1	mineralization					1783:1796	lower mineralization	1777:1796	lower mineralization	1777:1796	Results provide evidence that highly digestible foods can accelerate shell growth and lower mineralization in this species.
34958956	2	38	dep	%	407:407	arg1	fiber					415:419	crude fiber	409:419	14.2% crude fiber; 39.2% neutral detergent fiber	403:450	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	2	38	dep	%	407:407	arg1	%					426:426	39.2%	422:426	14.2% crude fiber; 39.2% neutral detergent fiber	403:450	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	2	39	from	starch	497:502	arg1	diets					373:377	two different diets	359:377	two different diets	359:377	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	2	39	from	starch	497:502	arg1	high					489:492	high	489:492	high	489:492	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	4	40	theme	starch	1257:1262	arg1	6.1 ± 1.8 g kg-1 day-1					1060:1081	6.1 ± 1.8 g kg-1 day-1	1060:1081	6.1 ± 1.8 g kg-1 day-1	1060:1081	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	40	theme	starch	1257:1262	arg1	8 ± 2 days					1197:1206	8 ± 2 days	1197:1206	8 ± 2 days	1197:1206	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	40	theme	starch	1257:1262	arg1	energy					968:973	digestible energy	957:973	digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05)	957:1042	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	40	theme	starch	1257:1262	arg1	5.0 ± 1.8 g kg-1 day-1					1087:1108	5.0 ± 1.8 g kg-1 day-1	1087:1108	5.0 ± 1.8 g kg-1 day-1	1087:1108	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	40	theme	starch	1257:1262	arg1	10 ± 2 days					1212:1222	10 ± 2 days	1212:1222	10 ± 2 days	1212:1222	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	40	theme	starch	1257:1262	arg1	transit					1130:1136	shorter transit	1122:1136	shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01)	1122:1174	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	40	theme	starch	1257:1262	arg1	113.9 ± 32.1 kJ kg-1 day-1					976:1001	113.9 ± 32.1 kJ kg-1 day-1	976:1001	113.9 ± 32.1 kJ kg-1 day-1	976:1001	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	40	theme	starch	1257:1262	arg1	3 ± 1 days					1139:1148	3 ± 1 days	1139:1148	3 ± 1 days	1139:1148	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	40	theme	starch	1257:1262	arg1	Da					1247:1248	higher Da	1240:1248	higher Da of DM, starch, NDF, and GE	1240:1275	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	40	theme	starch	1257:1262	arg1	DM					1056:1057	digestible DM	1045:1057	digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01)	1045:1119	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	40	theme	starch	1257:1262	arg1	times					1190:1194	retention times	1180:1194	retention times	1180:1194	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	40	theme	starch	1257:1262	arg1	99.6 ± 35.3 kJ kg-1 day-1					1007:1031	99.6 ± 35.3 kJ kg-1 day-1	1007:1031	99.6 ± 35.3 kJ kg-1 day-1	1007:1031	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	40	theme	starch	1257:1262	arg1	4 ± 1 days					1154:1163	4 ± 1 days	1154:1163	4 ± 1 days	1154:1163	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	3	41	theme	passage	736:742	arg1	times					744:748	passage times	736:748	passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition	736:901	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	41	theme	passage	736:742	arg1	coefficients					682:693	apparent digestibility coefficients	659:693	apparent digestibility coefficients (Da) of nutrients and gross energy (GE)	659:733	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	42	from	11 months	759:767	arg1	times					744:748	passage times	736:748	passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition	736:901	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	42	from	11 months	759:767	arg1	coefficients					682:693	apparent digestibility coefficients	659:693	apparent digestibility coefficients (Da) of nutrients and gross energy (GE)	659:733	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	42	from	11 months	759:767	arg1	6					822:822	6	822:822	6	822:822	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	42	from	11 months	759:767	arg1	12 months					828:836	12 months	828:836	12 months of age	828:843	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	4	43	theme	NDF	1265:1267	arg1	6.1 ± 1.8 g kg-1 day-1					1060:1081	6.1 ± 1.8 g kg-1 day-1	1060:1081	6.1 ± 1.8 g kg-1 day-1	1060:1081	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	43	theme	NDF	1265:1267	arg1	8 ± 2 days					1197:1206	8 ± 2 days	1197:1206	8 ± 2 days	1197:1206	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	43	theme	NDF	1265:1267	arg1	energy					968:973	digestible energy	957:973	digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05)	957:1042	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	43	theme	NDF	1265:1267	arg1	5.0 ± 1.8 g kg-1 day-1					1087:1108	5.0 ± 1.8 g kg-1 day-1	1087:1108	5.0 ± 1.8 g kg-1 day-1	1087:1108	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	43	theme	NDF	1265:1267	arg1	10 ± 2 days					1212:1222	10 ± 2 days	1212:1222	10 ± 2 days	1212:1222	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	43	theme	NDF	1265:1267	arg1	transit					1130:1136	shorter transit	1122:1136	shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01)	1122:1174	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	43	theme	NDF	1265:1267	arg1	113.9 ± 32.1 kJ kg-1 day-1					976:1001	113.9 ± 32.1 kJ kg-1 day-1	976:1001	113.9 ± 32.1 kJ kg-1 day-1	976:1001	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	43	theme	NDF	1265:1267	arg1	3 ± 1 days					1139:1148	3 ± 1 days	1139:1148	3 ± 1 days	1139:1148	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	43	theme	NDF	1265:1267	arg1	Da					1247:1248	higher Da	1240:1248	higher Da of DM, starch, NDF, and GE	1240:1275	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	43	theme	NDF	1265:1267	arg1	DM					1056:1057	digestible DM	1045:1057	digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01)	1045:1119	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	43	theme	NDF	1265:1267	arg1	times					1190:1194	retention times	1180:1194	retention times	1180:1194	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	43	theme	NDF	1265:1267	arg1	99.6 ± 35.3 kJ kg-1 day-1					1007:1031	99.6 ± 35.3 kJ kg-1 day-1	1007:1031	99.6 ± 35.3 kJ kg-1 day-1	1007:1031	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	43	theme	NDF	1265:1267	arg1	4 ± 1 days					1154:1163	4 ± 1 days	1154:1163	4 ± 1 days	1154:1163	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	3	44	theme	composition	891:901	arg1	11 months					759:767	11 months	759:767	11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition	759:901	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	5	45	theme	Crude	1278:1282	arg1	Da					1292:1293	Crude protein Da	1278:1293	Crude protein Da	1278:1293	Crude protein Da was higher for HF.
34958956	2	46	dep	HF	399:400	arg1	%					407:407	14.2%	403:407	14.2% crude fiber; 39.2% neutral detergent fiber	403:450	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	2	46	dep	HF	399:400	arg1	DMB					476:478	DMB	476:478	DMB	476:478	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	2	46	dep	HF	399:400	arg1	basis					469:473	dry matter basis	458:473	dry matter basis	458:473	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	2	46	dep	HF	399:400	arg1	NDF					453:455	NDF	453:455	NDF	453:455	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	0	47	theme	energy	35:40	arg1	metabolism					42:51	energy metabolism	35:51	energy metabolism	35:51	Starch and fiber intake effects on energy metabolism, growth, and carapacial scute pyramiding of red-footed tortoise hatchlings (Chelonoidis carbonaria).
34958956	2	48	from	high	384:387	arg1	fiber					392:396	fiber	392:396	fiber	392:396	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	3	49	from	12 months	828:836	arg1	rates					853:857	growth rates	846:857	growth rates	846:857	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	49	from	12 months	828:836	arg1	composition					891:901	estimated body composition	876:901	estimated body composition	876:901	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	49	from	12 months	828:836	arg1	11 months					759:767	11 months	759:767	11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition	759:901	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	49	from	12 months	828:836	arg1	pyramiding					860:869	pyramiding	860:869	pyramiding	860:869	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	49	from	12 months	828:836	arg1	age					772:774	age	772:774	age	772:774	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	49	from	12 months	828:836	arg1	rates					813:817	resting and post-prandial metabolic rates	777:817	resting and post-prandial metabolic rates at 6 and 12 months of age	777:843	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	9	50	theme	digestible	1728:1737	arg1	foods					1739:1743	highly digestible foods	1721:1743	highly digestible foods	1721:1743	Results provide evidence that highly digestible foods can accelerate shell growth and lower mineralization in this species.
34958956	1	51	theme	carapace	214:221	arg1	pyramiding					223:232	carapace pyramiding	214:232	carapace pyramiding	214:232	Tortoise husbandry includes reports of excessive growth and carapace pyramiding, although triggers still remain to be fully elucidated.
34958956	2	52	from	fiber	392:396	arg1	high					384:387	high	384:387	high	384:387	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	2	53	dep	high	384:387	arg1	HF					399:400	HF	399:400	HF	399:400	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	2	54	theme	different	363:371	arg1	one					380:382	one	380:382	one	380:382	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	2	54	theme	different	363:371	arg1	diets					373:377	two different diets	359:377	two different diets	359:377	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	2	54	theme	different	363:371	arg1	high					489:492	high	489:492	high	489:492	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	2	55	theme	HS	505:506	arg1	starch					497:502	starch	497:502	starch (HS; 27.7% DMB)	497:518	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	2	55	theme	HS	505:506	arg1	DMB					515:517	HS; 27.7% DMB	505:517	HS; 27.7% DMB	505:517	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	3	56	from	6	822:822	arg1	rates					853:857	growth rates	846:857	growth rates	846:857	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	56	from	6	822:822	arg1	composition					891:901	estimated body composition	876:901	estimated body composition	876:901	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	56	from	6	822:822	arg1	11 months					759:767	11 months	759:767	11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition	759:901	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	56	from	6	822:822	arg1	pyramiding					860:869	pyramiding	860:869	pyramiding	860:869	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	56	from	6	822:822	arg1	age					772:774	age	772:774	age	772:774	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	56	from	6	822:822	arg1	rates					813:817	resting and post-prandial metabolic rates	777:817	resting and post-prandial metabolic rates at 6 and 12 months of age	777:843	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	8	57	contain	had	1546:1548	arg2	%					1589:1589	1.88 ± 0.15%	1578:1589	1.88 ± 0.15%	1578:1589	In addition, these animals had lower body mineral content (1.88 ± 0.15% vs. 2.15 ± 0.19%; P < 0.01) and bone density (0.13 ± 0.01 g mm-2 vs. 0.15 ± 0.02 g mm-2; P < 0.02).
34958956	8	57	contain	had	1546:1548	arg2	density					1628:1634	bone density	1623:1634	bone density (0.13 ± 0.01 g mm-2 vs. 0.15 ± 0.02 g mm-2; P < 0.02)	1623:1688	In addition, these animals had lower body mineral content (1.88 ± 0.15% vs. 2.15 ± 0.19%; P < 0.01) and bone density (0.13 ± 0.01 g mm-2 vs. 0.15 ± 0.02 g mm-2; P < 0.02).
34958956	8	57	contain	had	1546:1548	arg2	0.13 ± 0.01 g mm-2					1637:1654	0.13 ± 0.01 g mm-2	1637:1654	0.13 ± 0.01 g mm-2	1637:1654	In addition, these animals had lower body mineral content (1.88 ± 0.15% vs. 2.15 ± 0.19%; P < 0.01) and bone density (0.13 ± 0.01 g mm-2 vs. 0.15 ± 0.02 g mm-2; P < 0.02).
34958956	8	57	contain	had	1546:1548	arg2	0.15 ± 0.02 g mm-2					1660:1677	0.15 ± 0.02 g mm-2	1660:1677	0.15 ± 0.02 g mm-2	1660:1677	In addition, these animals had lower body mineral content (1.88 ± 0.15% vs. 2.15 ± 0.19%; P < 0.01) and bone density (0.13 ± 0.01 g mm-2 vs. 0.15 ± 0.02 g mm-2; P < 0.02).
34958956	8	57	contain	had	1546:1548	arg1	animals					1538:1544	these animals	1532:1544	these animals	1532:1544	In addition, these animals had lower body mineral content (1.88 ± 0.15% vs. 2.15 ± 0.19%; P < 0.01) and bone density (0.13 ± 0.01 g mm-2 vs. 0.15 ± 0.02 g mm-2; P < 0.02).
34958956	8	57	contain	had	1546:1548	arg2	content					1569:1575	lower body mineral content	1550:1575	lower body mineral content (1.88 ± 0.15% vs. 2.15 ± 0.19%; P < 0.01)	1550:1617	In addition, these animals had lower body mineral content (1.88 ± 0.15% vs. 2.15 ± 0.19%; P < 0.01) and bone density (0.13 ± 0.01 g mm-2 vs. 0.15 ± 0.02 g mm-2; P < 0.02).
34958956	8	57	contain	had	1546:1548	arg2	%					1606:1606	2.15 ± 0.19%	1595:1606	2.15 ± 0.19%	1595:1606	In addition, these animals had lower body mineral content (1.88 ± 0.15% vs. 2.15 ± 0.19%; P < 0.01) and bone density (0.13 ± 0.01 g mm-2 vs. 0.15 ± 0.02 g mm-2; P < 0.02).
34958956	3	58	theme	metabolic	803:811	arg1	rates					813:817	resting and post-prandial metabolic rates	777:817	resting and post-prandial metabolic rates at 6 and 12 months of age	777:843	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	8	59	dep	%	1589:1589	arg1	P < 0.01					1609:1616	P < 0.01	1609:1616	P < 0.01	1609:1616	In addition, these animals had lower body mineral content (1.88 ± 0.15% vs. 2.15 ± 0.19%; P < 0.01) and bone density (0.13 ± 0.01 g mm-2 vs. 0.15 ± 0.02 g mm-2; P < 0.02).
34958956	2	60	from	high	489:492	arg1	starch					497:502	starch	497:502	starch (HS; 27.7% DMB)	497:518	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	2	60	from	high	489:492	arg1	DMB					515:517	HS; 27.7% DMB	505:517	HS; 27.7% DMB	505:517	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	4	61	theme	transit	1130:1136	arg1	intake					947:952	higher mass-specific intake	926:952	higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE	926:1275	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	1	62	theme	Tortoise	154:161	arg1	husbandry					163:171	Tortoise husbandry	154:171	Tortoise husbandry	154:171	Tortoise husbandry includes reports of excessive growth and carapace pyramiding, although triggers still remain to be fully elucidated.
34958956	4	63	theme	higher	926:931	arg1	intake					947:952	higher mass-specific intake	926:952	higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE	926:1275	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	8	64	theme	bone	1623:1626	arg1	0.13 ± 0.01 g mm-2					1637:1654	0.13 ± 0.01 g mm-2	1637:1654	0.13 ± 0.01 g mm-2	1637:1654	In addition, these animals had lower body mineral content (1.88 ± 0.15% vs. 2.15 ± 0.19%; P < 0.01) and bone density (0.13 ± 0.01 g mm-2 vs. 0.15 ± 0.02 g mm-2; P < 0.02).
34958956	8	64	theme	bone	1623:1626	arg1	density					1628:1634	bone density	1623:1634	bone density (0.13 ± 0.01 g mm-2 vs. 0.15 ± 0.02 g mm-2; P < 0.02)	1623:1688	In addition, these animals had lower body mineral content (1.88 ± 0.15% vs. 2.15 ± 0.19%; P < 0.01) and bone density (0.13 ± 0.01 g mm-2 vs. 0.15 ± 0.02 g mm-2; P < 0.02).
34958956	8	64	theme	bone	1623:1626	arg1	0.15 ± 0.02 g mm-2					1660:1677	0.15 ± 0.02 g mm-2	1660:1677	0.15 ± 0.02 g mm-2	1660:1677	In addition, these animals had lower body mineral content (1.88 ± 0.15% vs. 2.15 ± 0.19%; P < 0.01) and bone density (0.13 ± 0.01 g mm-2 vs. 0.15 ± 0.02 g mm-2; P < 0.02).
34958956	7	65	attach	presented	1438:1446	arg1	13 months					1407:1415	13 months	1407:1415	13 months	1407:1415	At 13 months, the animals from HS presented wider plastrons and carapaces, and higher carapace width growth rates.
34958956	7	65	attach	presented	1438:1446	arg2	animals					1422:1428	the animals	1418:1428	the animals from HS	1418:1436	At 13 months, the animals from HS presented wider plastrons and carapaces, and higher carapace width growth rates.
34958956	7	66	theme	growth	1505:1510	arg1	rates					1512:1516	higher carapace width growth rates	1483:1516	higher carapace width growth rates	1483:1516	At 13 months, the animals from HS presented wider plastrons and carapaces, and higher carapace width growth rates.
34958956	0	67	theme	tortoise	108:115	arg1	hatchlings					117:126	red-footed tortoise hatchlings	97:126	red-footed tortoise hatchlings (Chelonoidis carbonaria)	97:151	Starch and fiber intake effects on energy metabolism, growth, and carapacial scute pyramiding of red-footed tortoise hatchlings (Chelonoidis carbonaria).
34958956	0	67	theme	tortoise	108:115	arg1	carbonaria					141:150	carbonaria	141:150	carbonaria	141:150	Starch and fiber intake effects on energy metabolism, growth, and carapacial scute pyramiding of red-footed tortoise hatchlings (Chelonoidis carbonaria).
34958956	2	68	theme	red-footed	299:308	arg1	tortoises					310:318	Juvenile red-footed tortoises	290:318	Juvenile red-footed tortoises (Chelonoidis carbonaria)	290:343	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	4	69	dep	113.9 ± 32.1 kJ kg-1 day-1	976:1001	arg1	P < 0.05					1034:1041	P < 0.05	1034:1041	P < 0.05	1034:1041	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	3	70	dep	used	641:644	arg1	times					744:748	passage times	736:748	passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition	736:901	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	70	dep	used	641:644	arg1	Da					696:697	Da	696:697	Da	696:697	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	70	dep	used	641:644	arg1	coefficients					682:693	apparent digestibility coefficients	659:693	apparent digestibility coefficients (Da) of nutrients and gross energy (GE)	659:733	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	1	71	theme	growth	203:208	arg1	reports					182:188	reports	182:188	reports of excessive growth and carapace pyramiding	182:232	Tortoise husbandry includes reports of excessive growth and carapace pyramiding, although triggers still remain to be fully elucidated.
34958956	3	72	from	5	753:753	arg1	times					744:748	passage times	736:748	passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition	736:901	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	72	from	5	753:753	arg1	coefficients					682:693	apparent digestibility coefficients	659:693	apparent digestibility coefficients (Da) of nutrients and gross energy (GE)	659:733	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	7	73	theme	width	1499:1503	arg1	rates					1512:1516	higher carapace width growth rates	1483:1516	higher carapace width growth rates	1483:1516	At 13 months, the animals from HS presented wider plastrons and carapaces, and higher carapace width growth rates.
34958956	7	74	from	HS	1435:1436	arg1	animals					1422:1428	the animals	1418:1428	the animals from HS	1418:1436	At 13 months, the animals from HS presented wider plastrons and carapaces, and higher carapace width growth rates.
34958956	2	75	dep	tortoises	310:318	arg1	carbonaria					333:342	Chelonoidis carbonaria	321:342	Chelonoidis carbonaria	321:342	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	4	76	theme	higher	1240:1245	arg1	Da					1247:1248	higher Da	1240:1248	higher Da of DM, starch, NDF, and GE	1240:1275	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	6	77	theme	post-prandial	1323:1335	arg1	rates					1347:1351	post-prandial metabolic rates	1323:1351	post-prandial metabolic rates	1323:1351	Rest and post-prandial metabolic rates, and pyramiding degree were not affected by diets.
34958956	7	78	theme	higher	1483:1488	arg1	rates					1512:1516	higher carapace width growth rates	1483:1516	higher carapace width growth rates	1483:1516	At 13 months, the animals from HS presented wider plastrons and carapaces, and higher carapace width growth rates.
34958956	3	79	used	used	641:644	arg2	total					599:603	A total	597:603	A total of 20 hatchlings (10 per diet)	597:634	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	4	80	theme	digestible	957:966	arg1	99.6 ± 35.3 kJ kg-1 day-1					1007:1031	99.6 ± 35.3 kJ kg-1 day-1	1007:1031	99.6 ± 35.3 kJ kg-1 day-1	1007:1031	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	80	theme	digestible	957:966	arg1	113.9 ± 32.1 kJ kg-1 day-1					976:1001	113.9 ± 32.1 kJ kg-1 day-1	976:1001	113.9 ± 32.1 kJ kg-1 day-1	976:1001	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	80	theme	digestible	957:966	arg1	energy					968:973	digestible energy	957:973	digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05)	957:1042	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	3	81	theme	rates	853:857	arg1	11 months					759:767	11 months	759:767	11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition	759:901	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	82	theme	energy	723:728	arg1	times					744:748	passage times	736:748	passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition	736:901	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	82	theme	energy	723:728	arg1	Da					696:697	Da	696:697	Da	696:697	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	82	theme	energy	723:728	arg1	coefficients					682:693	apparent digestibility coefficients	659:693	apparent digestibility coefficients (Da) of nutrients and gross energy (GE)	659:733	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	2	83	theme	neutral	428:434	arg1	fiber					446:450	neutral detergent fiber	428:450	14.2% crude fiber; 39.2% neutral detergent fiber	403:450	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	4	84	theme	digestible	1045:1054	arg1	5.0 ± 1.8 g kg-1 day-1					1087:1108	5.0 ± 1.8 g kg-1 day-1	1087:1108	5.0 ± 1.8 g kg-1 day-1	1087:1108	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	84	theme	digestible	1045:1054	arg1	6.1 ± 1.8 g kg-1 day-1					1060:1081	6.1 ± 1.8 g kg-1 day-1	1060:1081	6.1 ± 1.8 g kg-1 day-1	1060:1081	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	84	theme	digestible	1045:1054	arg1	DM					1056:1057	digestible DM	1045:1057	digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01)	1045:1119	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	0	85	theme	scute	77:81	arg1	pyramiding					83:92	carapacial scute pyramiding	66:92	carapacial scute pyramiding of red-footed tortoise hatchlings (Chelonoidis carbonaria)	66:151	Starch and fiber intake effects on energy metabolism, growth, and carapacial scute pyramiding of red-footed tortoise hatchlings (Chelonoidis carbonaria).
34958956	2	86	dep	%	426:426	arg1	fiber					446:450	neutral detergent fiber	428:450	14.2% crude fiber; 39.2% neutral detergent fiber	403:450	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	2	87	theme	dry	458:460	arg1	basis					469:473	dry matter basis	458:473	dry matter basis	458:473	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	9	88	theme	shell	1760:1764	arg1	growth					1766:1771	shell growth	1760:1771	shell growth	1760:1771	Results provide evidence that highly digestible foods can accelerate shell growth and lower mineralization in this species.
34958956	3	89	theme	apparent	659:666	arg1	times					744:748	passage times	736:748	passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition	736:901	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	89	theme	apparent	659:666	arg1	Da					696:697	Da	696:697	Da	696:697	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	3	89	theme	apparent	659:666	arg1	coefficients					682:693	apparent digestibility coefficients	659:693	apparent digestibility coefficients (Da) of nutrients and gross energy (GE)	659:733	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	8	90	theme	body	1556:1559	arg1	%					1606:1606	2.15 ± 0.19%	1595:1606	2.15 ± 0.19%	1595:1606	In addition, these animals had lower body mineral content (1.88 ± 0.15% vs. 2.15 ± 0.19%; P < 0.01) and bone density (0.13 ± 0.01 g mm-2 vs. 0.15 ± 0.02 g mm-2; P < 0.02).
34958956	8	90	theme	body	1556:1559	arg1	content					1569:1575	lower body mineral content	1550:1575	lower body mineral content (1.88 ± 0.15% vs. 2.15 ± 0.19%; P < 0.01)	1550:1617	In addition, these animals had lower body mineral content (1.88 ± 0.15% vs. 2.15 ± 0.19%; P < 0.01) and bone density (0.13 ± 0.01 g mm-2 vs. 0.15 ± 0.02 g mm-2; P < 0.02).
34958956	8	90	theme	body	1556:1559	arg1	%					1589:1589	1.88 ± 0.15%	1578:1589	1.88 ± 0.15%	1578:1589	In addition, these animals had lower body mineral content (1.88 ± 0.15% vs. 2.15 ± 0.19%; P < 0.01) and bone density (0.13 ± 0.01 g mm-2 vs. 0.15 ± 0.02 g mm-2; P < 0.02).
34958956	3	91	theme	pyramiding	860:869	arg1	11 months					759:767	11 months	759:767	11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition	759:901	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	2	92	theme	energy	542:547	arg1	metabolism					549:558	energy metabolism	542:558	energy metabolism	542:558	Juvenile red-footed tortoises (Chelonoidis carbonaria) were fed with two different diets, one high in fiber (HF; 14.2% crude fiber; 39.2% neutral detergent fiber, NDF; dry matter basis, DMB) and one high in starch (HS; 27.7% DMB), to assess effects on energy metabolism, nutrient digestibility, and growth.
34958956	4	93	theme	GE	1274:1275	arg1	6.1 ± 1.8 g kg-1 day-1					1060:1081	6.1 ± 1.8 g kg-1 day-1	1060:1081	6.1 ± 1.8 g kg-1 day-1	1060:1081	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	93	theme	GE	1274:1275	arg1	8 ± 2 days					1197:1206	8 ± 2 days	1197:1206	8 ± 2 days	1197:1206	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	93	theme	GE	1274:1275	arg1	energy					968:973	digestible energy	957:973	digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05)	957:1042	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	93	theme	GE	1274:1275	arg1	5.0 ± 1.8 g kg-1 day-1					1087:1108	5.0 ± 1.8 g kg-1 day-1	1087:1108	5.0 ± 1.8 g kg-1 day-1	1087:1108	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	93	theme	GE	1274:1275	arg1	10 ± 2 days					1212:1222	10 ± 2 days	1212:1222	10 ± 2 days	1212:1222	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	93	theme	GE	1274:1275	arg1	transit					1130:1136	shorter transit	1122:1136	shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01)	1122:1174	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	93	theme	GE	1274:1275	arg1	113.9 ± 32.1 kJ kg-1 day-1					976:1001	113.9 ± 32.1 kJ kg-1 day-1	976:1001	113.9 ± 32.1 kJ kg-1 day-1	976:1001	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	93	theme	GE	1274:1275	arg1	3 ± 1 days					1139:1148	3 ± 1 days	1139:1148	3 ± 1 days	1139:1148	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	93	theme	GE	1274:1275	arg1	Da					1247:1248	higher Da	1240:1248	higher Da of DM, starch, NDF, and GE	1240:1275	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	93	theme	GE	1274:1275	arg1	DM					1056:1057	digestible DM	1045:1057	digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01)	1045:1119	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	93	theme	GE	1274:1275	arg1	times					1190:1194	retention times	1180:1194	retention times	1180:1194	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	93	theme	GE	1274:1275	arg1	99.6 ± 35.3 kJ kg-1 day-1					1007:1031	99.6 ± 35.3 kJ kg-1 day-1	1007:1031	99.6 ± 35.3 kJ kg-1 day-1	1007:1031	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	93	theme	GE	1274:1275	arg1	4 ± 1 days					1154:1163	4 ± 1 days	1154:1163	4 ± 1 days	1154:1163	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	3	94	theme	body	886:889	arg1	composition					891:901	estimated body composition	876:901	estimated body composition	876:901	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	8	95	dep	0.13 ± 0.01 g mm-2	1637:1654	arg1	P < 0.02					1680:1687	P < 0.02	1680:1687	P < 0.02	1680:1687	In addition, these animals had lower body mineral content (1.88 ± 0.15% vs. 2.15 ± 0.19%; P < 0.01) and bone density (0.13 ± 0.01 g mm-2 vs. 0.15 ± 0.02 g mm-2; P < 0.02).
34958956	4	96	theme	shorter	1122:1128	arg1	3 ± 1 days					1139:1148	3 ± 1 days	1139:1148	3 ± 1 days	1139:1148	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	96	theme	shorter	1122:1128	arg1	transit					1130:1136	shorter transit	1122:1136	shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01)	1122:1174	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	96	theme	shorter	1122:1128	arg1	10 ± 2 days					1212:1222	10 ± 2 days	1212:1222	10 ± 2 days	1212:1222	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	96	theme	shorter	1122:1128	arg1	8 ± 2 days					1197:1206	8 ± 2 days	1197:1206	8 ± 2 days	1197:1206	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	4	96	theme	shorter	1122:1128	arg1	4 ± 1 days					1154:1163	4 ± 1 days	1154:1163	4 ± 1 days	1154:1163	Animals fed HS showed higher mass-specific intake of digestible energy (113.9 ± 32.1 kJ kg-1 day-1 vs. 99.6 ± 35.3 kJ kg-1 day-1; P < 0.05), digestible DM (6.1 ± 1.8 g kg-1 day-1 vs. 5.0 ± 1.8 g kg-1 day-1; P < 0.01), shorter transit (3 ± 1 days vs. 4 ± 1 days; P < 0.01) and retention times (8 ± 2 days vs. 10 ± 2 days; P < 0.01), and higher Da of DM, starch, NDF, and GE.
34958956	3	97	theme	age	772:774	arg1	11 months					759:767	11 months	759:767	11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition	759:901	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34958956	5	98	theme	protein	1284:1290	arg1	Da					1292:1293	Crude protein Da	1278:1293	Crude protein Da	1278:1293	Crude protein Da was higher for HF.
34958956	1	99	theme	pyramiding	223:232	arg1	reports					182:188	reports	182:188	reports of excessive growth and carapace pyramiding	182:232	Tortoise husbandry includes reports of excessive growth and carapace pyramiding, although triggers still remain to be fully elucidated.
34958956	3	100	theme	post-prandial	789:801	arg1	rates					813:817	resting and post-prandial metabolic rates	777:817	resting and post-prandial metabolic rates at 6 and 12 months of age	777:843	A total of 20 hatchlings (10 per diet) were used to evaluate: apparent digestibility coefficients (Da) of nutrients and gross energy (GE), passage times at 5 and 11 months of age; resting and post-prandial metabolic rates at 6 and 12 months of age; growth rates; pyramiding; and estimated body composition.
34020817	7	0	theme	filtration	1437:1446	arg1	steps					1448:1452	initial and final tangential flow filtration steps	1403:1452	initial and final tangential flow filtration steps	1403:1452	BG505 DS-SOSIP.664 was expressed in a CHO-DG44 stable cell line and purified with initial and final tangential flow filtration steps, three commercially available resin-based chromatography steps, and two orthogonal viral clearance steps.
34020817	11	1	theme	Env	2138:2140	arg1	trimers					2142:2148	HIV-1 Env trimers	2132:2148	HIV-1 Env trimers from different clades	2132:2170	The successful process development and purification scale-up of HIV-1 Env trimers from different clades by using commercially available materials provide experimental demonstration for cGMP manufacturing of trimeric HIV-Env vaccine immunogens, in an antigenically desired conformation, without the use of costly affinity resins.
34020817	7	2	theme	tangential	1421:1430	arg1	steps					1448:1452	initial and final tangential flow filtration steps	1403:1452	initial and final tangential flow filtration steps	1403:1452	BG505 DS-SOSIP.664 was expressed in a CHO-DG44 stable cell line and purified with initial and final tangential flow filtration steps, three commercially available resin-based chromatography steps, and two orthogonal viral clearance steps.
34020817	6	3	theme	BG505	1127:1131	arg1	"					1145:1145	BG505 DS-SOSIP.664"	1127:1145	BG505 DS-SOSIP.664"	1127:1145	The clade A trimer, which we named "BG505 DS-SOSIP.664", contained an engineered disulfide (201C-433C; DS) within gp120, which further stabilized this trimer in a prefusion-closed conformation resistant to CD4-induced triggering.
34020817	11	4	theme	vaccine	2292:2298	arg1	immunogens					2300:2309	trimeric HIV-Env vaccine immunogens	2275:2309	trimeric HIV-Env vaccine immunogens	2275:2309	The successful process development and purification scale-up of HIV-1 Env trimers from different clades by using commercially available materials provide experimental demonstration for cGMP manufacturing of trimeric HIV-Env vaccine immunogens, in an antigenically desired conformation, without the use of costly affinity resins.
34020817	10	5	theme	candidate	1974:1982	arg1	production					1911:1920	50 L-scale production	1900:1920	50 L-scale production of a second prefusion-stabilized Env trimer vaccine candidate, ConC-FP8v2 RnS-3mut-2G-SOSIP.664, yielding 7.8 g of this consensus clade C trimer	1900:2065	The developed manufacturing process was suitable for 50 L-scale production of a second prefusion-stabilized Env trimer vaccine candidate, ConC-FP8v2 RnS-3mut-2G-SOSIP.664, yielding 7.8 g of this consensus clade C trimer.
34020817	8	6	theme	BG505	1673:1677	arg1	DS-SOSIP.664					1679:1690	purified BG505 DS-SOSIP.664	1664:1690	purified BG505 DS-SOSIP.664	1664:1690	The non-affinity purification enabled efficient scale-up, with a 250 L-scale cGMP run yielding 9.6 g of purified BG505 DS-SOSIP.664.
34020817	11	7	theme	available	2194:2202	arg1	materials					2204:2212	commercially available materials	2181:2212	commercially available materials	2181:2212	The successful process development and purification scale-up of HIV-1 Env trimers from different clades by using commercially available materials provide experimental demonstration for cGMP manufacturing of trimeric HIV-Env vaccine immunogens, in an antigenically desired conformation, without the use of costly affinity resins.
34020817	7	8	theme	stable	1368:1373	arg1	line					1380:1383	a CHO-DG44 stable cell line	1357:1383	a CHO-DG44 stable cell line	1357:1383	BG505 DS-SOSIP.664 was expressed in a CHO-DG44 stable cell line and purified with initial and final tangential flow filtration steps, three commercially available resin-based chromatography steps, and two orthogonal viral clearance steps.
34020817	4	9	theme	Ile-to-Pro	671:680	arg1	mutations					687:695	disulfide (SOS) and Ile-to-Pro (IP) mutations	651:695	mutations	687:695	Prior reported GMP manufacturing for a soluble trimer stabilized in a near-native state by disulfide (SOS) and Ile-to-Pro (IP) mutations has employed affinity methods based on antibody 2G12, which recognizes only ~30% of circulating HIV strains.
34020817	4	9	theme	Ile-to-Pro	671:680	arg1	IP					683:684	IP	683:684	IP	683:684	Prior reported GMP manufacturing for a soluble trimer stabilized in a near-native state by disulfide (SOS) and Ile-to-Pro (IP) mutations has employed affinity methods based on antibody 2G12, which recognizes only ~30% of circulating HIV strains.
34020817	10	10	theme	trimer	1959:1964	arg1	RnS-3mut-2G-SOSIP.664					1996:2016	ConC-FP8v2 RnS-3mut-2G-SOSIP.664	1985:2016	ConC-FP8v2 RnS-3mut-2G-SOSIP.664	1985:2016	The developed manufacturing process was suitable for 50 L-scale production of a second prefusion-stabilized Env trimer vaccine candidate, ConC-FP8v2 RnS-3mut-2G-SOSIP.664, yielding 7.8 g of this consensus clade C trimer.
34020817	10	10	theme	trimer	1959:1964	arg1	candidate					1974:1982	a second prefusion-stabilized Env trimer vaccine candidate	1925:1982	a second prefusion-stabilized Env trimer vaccine candidate	1925:1982	The developed manufacturing process was suitable for 50 L-scale production of a second prefusion-stabilized Env trimer vaccine candidate, ConC-FP8v2 RnS-3mut-2G-SOSIP.664, yielding 7.8 g of this consensus clade C trimer.
34020817	5	11	from	production	957:966	arg1	A					991:991	A	991:991	A	991:991	Here, we develop a scalable manufacturing process based on commercially available, non-affinity resins, and we apply the process to current GMP (cGMP) production of trimers from clades A and C, which have been found to boost cross-clade neutralizing responses in vaccine-test species.
34020817	5	11	from	production	957:966	arg1	clades					984:989	clades A and C	984:997	clades A and C	984:997	Here, we develop a scalable manufacturing process based on commercially available, non-affinity resins, and we apply the process to current GMP (cGMP) production of trimers from clades A and C, which have been found to boost cross-clade neutralizing responses in vaccine-test species.
34020817	5	11	from	production	957:966	arg1	C					997:997	C	997:997	C	997:997	Here, we develop a scalable manufacturing process based on commercially available, non-affinity resins, and we apply the process to current GMP (cGMP) production of trimers from clades A and C, which have been found to boost cross-clade neutralizing responses in vaccine-test species.
34020817	5	12	dep	clades	984:989	arg1	A					991:991	A	991:991	A	991:991	Here, we develop a scalable manufacturing process based on commercially available, non-affinity resins, and we apply the process to current GMP (cGMP) production of trimers from clades A and C, which have been found to boost cross-clade neutralizing responses in vaccine-test species.
34020817	5	12	dep	clades	984:989	arg1	clades					984:989	clades A and C	984:997	clades A and C	984:997	Here, we develop a scalable manufacturing process based on commercially available, non-affinity resins, and we apply the process to current GMP (cGMP) production of trimers from clades A and C, which have been found to boost cross-clade neutralizing responses in vaccine-test species.
34020817	5	12	dep	clades	984:989	arg1	C					997:997	C	997:997	C	997:997	Here, we develop a scalable manufacturing process based on commercially available, non-affinity resins, and we apply the process to current GMP (cGMP) production of trimers from clades A and C, which have been found to boost cross-clade neutralizing responses in vaccine-test species.
34020817	2	13	link	N-linked	279:286	arg1	glycan					288:293	N-linked glycan	279:293	N-linked glycan comprising half its mass	279:318	The HIV-1 envelope (Env) glycoprotein trimer is covered by N-linked glycan comprising half its mass and requires both trimer assembly and subunit cleavage to fold into a prefusion-closed conformation.
34020817	11	14	theme	process	2083:2089	arg1	development					2091:2101	successful process development	2072:2101	successful process development	2072:2101	The successful process development and purification scale-up of HIV-1 Env trimers from different clades by using commercially available materials provide experimental demonstration for cGMP manufacturing of trimeric HIV-Env vaccine immunogens, in an antigenically desired conformation, without the use of costly affinity resins.
34020817	1	15	theme	GMP	201:203	arg1	manufacturing					205:217	GMP manufacturing	201:217	GMP manufacturing	201:217	Metastable glycosylated immunogens present challenges for GMP manufacturing.
34020817	11	16	theme	purification	2107:2118	arg1	scale-up					2120:2127	purification scale-up	2107:2127	purification scale-up	2107:2127	The successful process development and purification scale-up of HIV-1 Env trimers from different clades by using commercially available materials provide experimental demonstration for cGMP manufacturing of trimeric HIV-Env vaccine immunogens, in an antigenically desired conformation, without the use of costly affinity resins.
34020817	11	17	theme	affinity	2380:2387	arg1	resins					2389:2394	costly affinity resins	2373:2394	costly affinity resins	2373:2394	The successful process development and purification scale-up of HIV-1 Env trimers from different clades by using commercially available materials provide experimental demonstration for cGMP manufacturing of trimeric HIV-Env vaccine immunogens, in an antigenically desired conformation, without the use of costly affinity resins.
34020817	10	18	theme	ConC-FP8v2	1985:1994	arg1	RnS-3mut-2G-SOSIP.664					1996:2016	ConC-FP8v2 RnS-3mut-2G-SOSIP.664	1985:2016	ConC-FP8v2 RnS-3mut-2G-SOSIP.664	1985:2016	The developed manufacturing process was suitable for 50 L-scale production of a second prefusion-stabilized Env trimer vaccine candidate, ConC-FP8v2 RnS-3mut-2G-SOSIP.664, yielding 7.8 g of this consensus clade C trimer.
34020817	10	18	theme	ConC-FP8v2	1985:1994	arg1	candidate					1974:1982	a second prefusion-stabilized Env trimer vaccine candidate	1925:1982	a second prefusion-stabilized Env trimer vaccine candidate	1925:1982	The developed manufacturing process was suitable for 50 L-scale production of a second prefusion-stabilized Env trimer vaccine candidate, ConC-FP8v2 RnS-3mut-2G-SOSIP.664, yielding 7.8 g of this consensus clade C trimer.
34020817	6	19	theme	prefusion-closed	1254:1269	arg1	conformation					1271:1282	a prefusion-closed conformation	1252:1282	a prefusion-closed conformation resistant to CD4-induced triggering	1252:1318	The clade A trimer, which we named "BG505 DS-SOSIP.664", contained an engineered disulfide (201C-433C; DS) within gp120, which further stabilized this trimer in a prefusion-closed conformation resistant to CD4-induced triggering.
34020817	5	20	from	clades	984:989	arg1	trimers					971:977	trimers	971:977	trimers from clades A and C	971:997	Here, we develop a scalable manufacturing process based on commercially available, non-affinity resins, and we apply the process to current GMP (cGMP) production of trimers from clades A and C, which have been found to boost cross-clade neutralizing responses in vaccine-test species.
34020817	5	20	from	clades	984:989	arg1	production					957:966	current GMP (cGMP) production	938:966	current GMP (cGMP) production	938:966	Here, we develop a scalable manufacturing process based on commercially available, non-affinity resins, and we apply the process to current GMP (cGMP) production of trimers from clades A and C, which have been found to boost cross-clade neutralizing responses in vaccine-test species.
34020817	4	21	theme	affinity	710:717	arg1	methods					719:725	affinity methods	710:725	affinity methods based on antibody 2G12	710:748	Prior reported GMP manufacturing for a soluble trimer stabilized in a near-native state by disulfide (SOS) and Ile-to-Pro (IP) mutations has employed affinity methods based on antibody 2G12, which recognizes only ~30% of circulating HIV strains.
34020817	0	22	theme	clinical	122:129	arg1	evaluation					131:140	clinical evaluation	122:140	clinical evaluation	122:140	A non-affinity purification process for GMP production of prefusion-closed HIV-1 envelope trimers from clades A and C for clinical evaluation.
34020817	2	23	theme	Env	240:242	arg1	glycoprotein					245:256	The HIV-1 envelope (Env) glycoprotein	220:256	The HIV-1 envelope (Env) glycoprotein trimer	220:263	The HIV-1 envelope (Env) glycoprotein trimer is covered by N-linked glycan comprising half its mass and requires both trimer assembly and subunit cleavage to fold into a prefusion-closed conformation.
34020817	5	24	theme	manufacturing	834:846	arg1	process					848:854	a scalable manufacturing process	823:854	a scalable manufacturing process based on commercially available, non-affinity resins	823:907	Here, we develop a scalable manufacturing process based on commercially available, non-affinity resins, and we apply the process to current GMP (cGMP) production of trimers from clades A and C, which have been found to boost cross-clade neutralizing responses in vaccine-test species.
34020817	9	25	theme	Antigenic	1693:1701	arg1	analysis					1703:1710	Antigenic analysis	1693:1710	Antigenic analysis	1693:1710	Antigenic analysis indicated retention of a prefusion-closed conformation, including recognition by apex-directed and fusion peptide-directed antibodies.
34020817	6	26	theme	engineered	1161:1170	arg1	201C-433C					1183:1191	201C-433C	1183:1191	201C-433C	1183:1191	The clade A trimer, which we named "BG505 DS-SOSIP.664", contained an engineered disulfide (201C-433C; DS) within gp120, which further stabilized this trimer in a prefusion-closed conformation resistant to CD4-induced triggering.
34020817	6	26	theme	engineered	1161:1170	arg1	disulfide					1172:1180	an engineered disulfide	1158:1180	an engineered disulfide (201C-433C; DS)	1158:1196	The clade A trimer, which we named "BG505 DS-SOSIP.664", contained an engineered disulfide (201C-433C; DS) within gp120, which further stabilized this trimer in a prefusion-closed conformation resistant to CD4-induced triggering.
34020817	11	27	from	clades	2165:2170	arg1	development					2091:2101	successful process development	2072:2101	successful process development	2072:2101	The successful process development and purification scale-up of HIV-1 Env trimers from different clades by using commercially available materials provide experimental demonstration for cGMP manufacturing of trimeric HIV-Env vaccine immunogens, in an antigenically desired conformation, without the use of costly affinity resins.
34020817	11	27	from	clades	2165:2170	arg1	scale-up					2120:2127	purification scale-up	2107:2127	purification scale-up	2107:2127	The successful process development and purification scale-up of HIV-1 Env trimers from different clades by using commercially available materials provide experimental demonstration for cGMP manufacturing of trimeric HIV-Env vaccine immunogens, in an antigenically desired conformation, without the use of costly affinity resins.
34020817	11	27	from	clades	2165:2170	arg1	trimers					2142:2148	HIV-1 Env trimers	2132:2148	HIV-1 Env trimers from different clades	2132:2170	The successful process development and purification scale-up of HIV-1 Env trimers from different clades by using commercially available materials provide experimental demonstration for cGMP manufacturing of trimeric HIV-Env vaccine immunogens, in an antigenically desired conformation, without the use of costly affinity resins.
34020817	2	28	theme	envelope	230:237	arg1	glycoprotein					245:256	The HIV-1 envelope (Env) glycoprotein	220:256	The HIV-1 envelope (Env) glycoprotein trimer	220:263	The HIV-1 envelope (Env) glycoprotein trimer is covered by N-linked glycan comprising half its mass and requires both trimer assembly and subunit cleavage to fold into a prefusion-closed conformation.
34020817	10	29	theme	trimer	2060:2065	arg1	7.8 g					2028:2032	7.8 g	2028:2032	7.8 g of this consensus clade C trimer	2028:2065	The developed manufacturing process was suitable for 50 L-scale production of a second prefusion-stabilized Env trimer vaccine candidate, ConC-FP8v2 RnS-3mut-2G-SOSIP.664, yielding 7.8 g of this consensus clade C trimer.
34020817	1	30	gly	glycosylated	154:165	arg1	immunogens					167:176	Metastable glycosylated immunogens	143:176	Metastable glycosylated immunogens	143:176	Metastable glycosylated immunogens present challenges for GMP manufacturing.
34020817	11	31	theme	immunogens	2300:2309	arg1	manufacturing					2258:2270	cGMP manufacturing	2253:2270	cGMP manufacturing of trimeric HIV-Env vaccine immunogens	2253:2309	The successful process development and purification scale-up of HIV-1 Env trimers from different clades by using commercially available materials provide experimental demonstration for cGMP manufacturing of trimeric HIV-Env vaccine immunogens, in an antigenically desired conformation, without the use of costly affinity resins.
34020817	10	32	theme	clade	2052:2056	arg1	trimer					2060:2065	this consensus clade C trimer	2037:2065	this consensus clade C trimer	2037:2065	The developed manufacturing process was suitable for 50 L-scale production of a second prefusion-stabilized Env trimer vaccine candidate, ConC-FP8v2 RnS-3mut-2G-SOSIP.664, yielding 7.8 g of this consensus clade C trimer.
34020817	0	33	theme	prefusion-closed	58:73	arg1	trimers					90:96	prefusion-closed HIV-1 envelope trimers	58:96	prefusion-closed HIV-1 envelope trimers from clades A and C	58:116	A non-affinity purification process for GMP production of prefusion-closed HIV-1 envelope trimers from clades A and C for clinical evaluation.
34020817	5	34	from	responses	1056:1064	arg1	species					1082:1088	vaccine-test species	1069:1088	vaccine-test species	1069:1088	Here, we develop a scalable manufacturing process based on commercially available, non-affinity resins, and we apply the process to current GMP (cGMP) production of trimers from clades A and C, which have been found to boost cross-clade neutralizing responses in vaccine-test species.
34020817	1	35	theme	Metastable	143:152	arg1	immunogens					167:176	Metastable glycosylated immunogens	143:176	Metastable glycosylated immunogens	143:176	Metastable glycosylated immunogens present challenges for GMP manufacturing.
34020817	7	36	theme	clearance	1543:1551	arg1	steps					1553:1557	two orthogonal viral clearance steps	1522:1557	two orthogonal viral clearance steps	1522:1557	BG505 DS-SOSIP.664 was expressed in a CHO-DG44 stable cell line and purified with initial and final tangential flow filtration steps, three commercially available resin-based chromatography steps, and two orthogonal viral clearance steps.
34020817	9	37	theme	prefusion-closed	1737:1752	arg1	conformation					1754:1765	a prefusion-closed conformation	1735:1765	a prefusion-closed conformation	1735:1765	Antigenic analysis indicated retention of a prefusion-closed conformation, including recognition by apex-directed and fusion peptide-directed antibodies.
34020817	11	38	from	development	2091:2101	arg1	clades					2165:2170	different clades	2155:2170	different clades	2155:2170	The successful process development and purification scale-up of HIV-1 Env trimers from different clades by using commercially available materials provide experimental demonstration for cGMP manufacturing of trimeric HIV-Env vaccine immunogens, in an antigenically desired conformation, without the use of costly affinity resins.
34020817	0	39	theme	envelope	81:88	arg1	trimers					90:96	prefusion-closed HIV-1 envelope trimers	58:96	prefusion-closed HIV-1 envelope trimers from clades A and C	58:116	A non-affinity purification process for GMP production of prefusion-closed HIV-1 envelope trimers from clades A and C for clinical evaluation.
34020817	7	40	theme	orthogonal	1526:1535	arg1	steps					1553:1557	two orthogonal viral clearance steps	1522:1557	two orthogonal viral clearance steps	1522:1557	BG505 DS-SOSIP.664 was expressed in a CHO-DG44 stable cell line and purified with initial and final tangential flow filtration steps, three commercially available resin-based chromatography steps, and two orthogonal viral clearance steps.
34020817	6	41	theme	CD4-induced	1297:1307	arg1	triggering					1309:1318	CD4-induced triggering	1297:1318	CD4-induced triggering	1297:1318	The clade A trimer, which we named "BG505 DS-SOSIP.664", contained an engineered disulfide (201C-433C; DS) within gp120, which further stabilized this trimer in a prefusion-closed conformation resistant to CD4-induced triggering.
34020817	5	42	theme	neutralizing	1043:1054	arg1	responses					1056:1064	cross-clade neutralizing responses	1031:1064	cross-clade neutralizing responses in vaccine-test species	1031:1088	Here, we develop a scalable manufacturing process based on commercially available, non-affinity resins, and we apply the process to current GMP (cGMP) production of trimers from clades A and C, which have been found to boost cross-clade neutralizing responses in vaccine-test species.
34020817	0	43	from	production	44:53	arg1	clades					103:108	clades A and C	103:116	clades A and C	103:116	A non-affinity purification process for GMP production of prefusion-closed HIV-1 envelope trimers from clades A and C for clinical evaluation.
34020817	0	43	from	production	44:53	arg1	C					116:116	C	116:116	C	116:116	A non-affinity purification process for GMP production of prefusion-closed HIV-1 envelope trimers from clades A and C for clinical evaluation.
34020817	0	43	from	production	44:53	arg1	A					110:110	A	110:110	A	110:110	A non-affinity purification process for GMP production of prefusion-closed HIV-1 envelope trimers from clades A and C for clinical evaluation.
34020817	2	44	theme	prefusion-closed	390:405	arg1	conformation					407:418	a prefusion-closed conformation	388:418	a prefusion-closed conformation	388:418	The HIV-1 envelope (Env) glycoprotein trimer is covered by N-linked glycan comprising half its mass and requires both trimer assembly and subunit cleavage to fold into a prefusion-closed conformation.
34020817	6	45	theme	resistant	1284:1292	arg1	conformation					1271:1282	a prefusion-closed conformation	1252:1282	a prefusion-closed conformation resistant to CD4-induced triggering	1252:1318	The clade A trimer, which we named "BG505 DS-SOSIP.664", contained an engineered disulfide (201C-433C; DS) within gp120, which further stabilized this trimer in a prefusion-closed conformation resistant to CD4-induced triggering.
34020817	9	46	theme	apex-directed	1793:1805	arg1	antibodies					1835:1844	apex-directed and fusion peptide-directed antibodies	1793:1844	apex-directed and fusion peptide-directed antibodies	1793:1844	Antigenic analysis indicated retention of a prefusion-closed conformation, including recognition by apex-directed and fusion peptide-directed antibodies.
34020817	4	47	theme	GMP	575:577	arg1	manufacturing					579:591	Prior reported GMP manufacturing	560:591	Prior reported GMP manufacturing for a soluble trimer stabilized in a near-native state by disulfide (SOS) and Ile-to-Pro (IP) mutations	560:695	Prior reported GMP manufacturing for a soluble trimer stabilized in a near-native state by disulfide (SOS) and Ile-to-Pro (IP) mutations has employed affinity methods based on antibody 2G12, which recognizes only ~30% of circulating HIV strains.
34020817	5	48	dep	available	878:886	arg1	non-affinity					889:900	non-affinity	889:900	non-affinity	889:900	Here, we develop a scalable manufacturing process based on commercially available, non-affinity resins, and we apply the process to current GMP (cGMP) production of trimers from clades A and C, which have been found to boost cross-clade neutralizing responses in vaccine-test species.
34020817	0	49	theme	purification	15:26	arg1	process					28:34	A non-affinity purification process	0:34	A non-affinity purification process for GMP production of prefusion-closed HIV-1 envelope trimers from clades A and C for clinical evaluation.	0:141	A non-affinity purification process for GMP production of prefusion-closed HIV-1 envelope trimers from clades A and C for clinical evaluation.
34020817	7	50	theme	chromatography	1496:1509	arg1	steps					1511:1515	three commercially available resin-based chromatography steps	1455:1515	three commercially available resin-based chromatography steps	1455:1515	BG505 DS-SOSIP.664 was expressed in a CHO-DG44 stable cell line and purified with initial and final tangential flow filtration steps, three commercially available resin-based chromatography steps, and two orthogonal viral clearance steps.
34020817	5	51	theme	current	938:944	arg1	cGMP					951:954	cGMP	951:954	cGMP	951:954	Here, we develop a scalable manufacturing process based on commercially available, non-affinity resins, and we apply the process to current GMP (cGMP) production of trimers from clades A and C, which have been found to boost cross-clade neutralizing responses in vaccine-test species.
34020817	5	51	theme	current	938:944	arg1	GMP					946:948	current GMP	938:948	current GMP (cGMP) production	938:966	Here, we develop a scalable manufacturing process based on commercially available, non-affinity resins, and we apply the process to current GMP (cGMP) production of trimers from clades A and C, which have been found to boost cross-clade neutralizing responses in vaccine-test species.
34020817	11	52	theme	trimeric	2275:2282	arg1	immunogens					2300:2309	trimeric HIV-Env vaccine immunogens	2275:2309	trimeric HIV-Env vaccine immunogens	2275:2309	The successful process development and purification scale-up of HIV-1 Env trimers from different clades by using commercially available materials provide experimental demonstration for cGMP manufacturing of trimeric HIV-Env vaccine immunogens, in an antigenically desired conformation, without the use of costly affinity resins.
34020817	7	53	theme	available	1474:1482	arg1	steps					1511:1515	three commercially available resin-based chromatography steps	1455:1515	three commercially available resin-based chromatography steps	1455:1515	BG505 DS-SOSIP.664 was expressed in a CHO-DG44 stable cell line and purified with initial and final tangential flow filtration steps, three commercially available resin-based chromatography steps, and two orthogonal viral clearance steps.
34020817	11	54	theme	cGMP	2253:2256	arg1	manufacturing					2258:2270	cGMP manufacturing	2253:2270	cGMP manufacturing of trimeric HIV-Env vaccine immunogens	2253:2309	The successful process development and purification scale-up of HIV-1 Env trimers from different clades by using commercially available materials provide experimental demonstration for cGMP manufacturing of trimeric HIV-Env vaccine immunogens, in an antigenically desired conformation, without the use of costly affinity resins.
34020817	10	55	theme	manufacturing	1861:1873	arg1	suitable					1887:1894	suitable	1887:1894	suitable	1887:1894	The developed manufacturing process was suitable for 50 L-scale production of a second prefusion-stabilized Env trimer vaccine candidate, ConC-FP8v2 RnS-3mut-2G-SOSIP.664, yielding 7.8 g of this consensus clade C trimer.
34020817	10	55	theme	manufacturing	1861:1873	arg1	process					1875:1881	The developed manufacturing process	1847:1881	The developed manufacturing process	1847:1881	The developed manufacturing process was suitable for 50 L-scale production of a second prefusion-stabilized Env trimer vaccine candidate, ConC-FP8v2 RnS-3mut-2G-SOSIP.664, yielding 7.8 g of this consensus clade C trimer.
34020817	3	56	theme	structural	499:508	arg1	rearrangement					510:522	structural rearrangement	499:522	structural rearrangement	499:522	This conformation, the vaccine-desired antigenic state, is both metastable to structural rearrangement and labile to subunit dissociation.
34020817	4	57	theme	Prior	560:564	arg1	manufacturing					579:591	Prior reported GMP manufacturing	560:591	Prior reported GMP manufacturing for a soluble trimer stabilized in a near-native state by disulfide (SOS) and Ile-to-Pro (IP) mutations	560:695	Prior reported GMP manufacturing for a soluble trimer stabilized in a near-native state by disulfide (SOS) and Ile-to-Pro (IP) mutations has employed affinity methods based on antibody 2G12, which recognizes only ~30% of circulating HIV strains.
34020817	10	58	theme	L-scale	1903:1909	arg1	production					1911:1920	50 L-scale production	1900:1920	50 L-scale production of a second prefusion-stabilized Env trimer vaccine candidate, ConC-FP8v2 RnS-3mut-2G-SOSIP.664, yielding 7.8 g of this consensus clade C trimer	1900:2065	The developed manufacturing process was suitable for 50 L-scale production of a second prefusion-stabilized Env trimer vaccine candidate, ConC-FP8v2 RnS-3mut-2G-SOSIP.664, yielding 7.8 g of this consensus clade C trimer.
34020817	4	59	theme	circulating	781:791	arg1	strains					797:803	circulating HIV strains	781:803	circulating HIV strains	781:803	Prior reported GMP manufacturing for a soluble trimer stabilized in a near-native state by disulfide (SOS) and Ile-to-Pro (IP) mutations has employed affinity methods based on antibody 2G12, which recognizes only ~30% of circulating HIV strains.
34020817	11	60	theme	resins	2389:2394	arg1	use					2366:2368	the use	2362:2368	the use of costly affinity resins	2362:2394	The successful process development and purification scale-up of HIV-1 Env trimers from different clades by using commercially available materials provide experimental demonstration for cGMP manufacturing of trimeric HIV-Env vaccine immunogens, in an antigenically desired conformation, without the use of costly affinity resins.
34020817	11	61	dep	development	2091:2101	arg1	The					2068:2070	The	2068:2070	The	2068:2070	The successful process development and purification scale-up of HIV-1 Env trimers from different clades by using commercially available materials provide experimental demonstration for cGMP manufacturing of trimeric HIV-Env vaccine immunogens, in an antigenically desired conformation, without the use of costly affinity resins.
34020817	6	62	dep	clade	1095:1099	arg1	trimer					1103:1108	A trimer	1101:1108	The clade A trimer	1091:1108	The clade A trimer, which we named "BG505 DS-SOSIP.664", contained an engineered disulfide (201C-433C; DS) within gp120, which further stabilized this trimer in a prefusion-closed conformation resistant to CD4-induced triggering.
34020817	8	63	theme	L-scale	1629:1635	arg1	run					1642:1644	a 250 L-scale cGMP run	1623:1644	a 250 L-scale cGMP run yielding 9.6 g of purified BG505 DS-SOSIP.664	1623:1690	The non-affinity purification enabled efficient scale-up, with a 250 L-scale cGMP run yielding 9.6 g of purified BG505 DS-SOSIP.664.
34020817	6	64	dep	201C-433C	1183:1191	arg1	DS					1194:1195	DS	1194:1195	DS	1194:1195	The clade A trimer, which we named "BG505 DS-SOSIP.664", contained an engineered disulfide (201C-433C; DS) within gp120, which further stabilized this trimer in a prefusion-closed conformation resistant to CD4-induced triggering.
34020817	7	65	theme	flow	1432:1435	arg1	steps					1448:1452	initial and final tangential flow filtration steps	1403:1452	initial and final tangential flow filtration steps	1403:1452	BG505 DS-SOSIP.664 was expressed in a CHO-DG44 stable cell line and purified with initial and final tangential flow filtration steps, three commercially available resin-based chromatography steps, and two orthogonal viral clearance steps.
34020817	3	66	dep	both	480:483	arg1	metastable					485:494	metastable	485:494	metastable	485:494	This conformation, the vaccine-desired antigenic state, is both metastable to structural rearrangement and labile to subunit dissociation.
34020817	10	67	theme	second	1927:1932	arg1	RnS-3mut-2G-SOSIP.664					1996:2016	ConC-FP8v2 RnS-3mut-2G-SOSIP.664	1985:2016	ConC-FP8v2 RnS-3mut-2G-SOSIP.664	1985:2016	The developed manufacturing process was suitable for 50 L-scale production of a second prefusion-stabilized Env trimer vaccine candidate, ConC-FP8v2 RnS-3mut-2G-SOSIP.664, yielding 7.8 g of this consensus clade C trimer.
34020817	10	67	theme	second	1927:1932	arg1	candidate					1974:1982	a second prefusion-stabilized Env trimer vaccine candidate	1925:1982	a second prefusion-stabilized Env trimer vaccine candidate	1925:1982	The developed manufacturing process was suitable for 50 L-scale production of a second prefusion-stabilized Env trimer vaccine candidate, ConC-FP8v2 RnS-3mut-2G-SOSIP.664, yielding 7.8 g of this consensus clade C trimer.
34020817	2	68	theme	subunit	358:364	arg1	cleavage					366:373	subunit cleavage	358:373	subunit cleavage	358:373	The HIV-1 envelope (Env) glycoprotein trimer is covered by N-linked glycan comprising half its mass and requires both trimer assembly and subunit cleavage to fold into a prefusion-closed conformation.
34020817	7	69	theme	final	1415:1419	arg1	steps					1448:1452	initial and final tangential flow filtration steps	1403:1452	initial and final tangential flow filtration steps	1403:1452	BG505 DS-SOSIP.664 was expressed in a CHO-DG44 stable cell line and purified with initial and final tangential flow filtration steps, three commercially available resin-based chromatography steps, and two orthogonal viral clearance steps.
34020817	4	70	theme	soluble	599:605	arg1	trimer					607:612	a soluble trimer	597:612	a soluble trimer stabilized in a near-native state by disulfide (SOS) and Ile-to-Pro (IP) mutations	597:695	Prior reported GMP manufacturing for a soluble trimer stabilized in a near-native state by disulfide (SOS) and Ile-to-Pro (IP) mutations has employed affinity methods based on antibody 2G12, which recognizes only ~30% of circulating HIV strains.
34020817	7	71	theme	initial	1403:1409	arg1	steps					1448:1452	initial and final tangential flow filtration steps	1403:1452	initial and final tangential flow filtration steps	1403:1452	BG505 DS-SOSIP.664 was expressed in a CHO-DG44 stable cell line and purified with initial and final tangential flow filtration steps, three commercially available resin-based chromatography steps, and two orthogonal viral clearance steps.
34020817	11	72	theme	different	2155:2163	arg1	clades					2165:2170	different clades	2155:2170	different clades	2155:2170	The successful process development and purification scale-up of HIV-1 Env trimers from different clades by using commercially available materials provide experimental demonstration for cGMP manufacturing of trimeric HIV-Env vaccine immunogens, in an antigenically desired conformation, without the use of costly affinity resins.
34020817	11	73	theme	experimental	2222:2233	arg1	demonstration					2235:2247	experimental demonstration	2222:2247	experimental demonstration	2222:2247	The successful process development and purification scale-up of HIV-1 Env trimers from different clades by using commercially available materials provide experimental demonstration for cGMP manufacturing of trimeric HIV-Env vaccine immunogens, in an antigenically desired conformation, without the use of costly affinity resins.
34020817	10	74	theme	vaccine	1966:1972	arg1	RnS-3mut-2G-SOSIP.664					1996:2016	ConC-FP8v2 RnS-3mut-2G-SOSIP.664	1985:2016	ConC-FP8v2 RnS-3mut-2G-SOSIP.664	1985:2016	The developed manufacturing process was suitable for 50 L-scale production of a second prefusion-stabilized Env trimer vaccine candidate, ConC-FP8v2 RnS-3mut-2G-SOSIP.664, yielding 7.8 g of this consensus clade C trimer.
34020817	10	74	theme	vaccine	1966:1972	arg1	candidate					1974:1982	a second prefusion-stabilized Env trimer vaccine candidate	1925:1982	a second prefusion-stabilized Env trimer vaccine candidate	1925:1982	The developed manufacturing process was suitable for 50 L-scale production of a second prefusion-stabilized Env trimer vaccine candidate, ConC-FP8v2 RnS-3mut-2G-SOSIP.664, yielding 7.8 g of this consensus clade C trimer.
34020817	2	75	theme	N-linked	279:286	arg1	glycan					288:293	N-linked glycan	279:293	N-linked glycan comprising half its mass	279:318	The HIV-1 envelope (Env) glycoprotein trimer is covered by N-linked glycan comprising half its mass and requires both trimer assembly and subunit cleavage to fold into a prefusion-closed conformation.
34020817	8	76	theme	purified	1664:1671	arg1	DS-SOSIP.664					1679:1690	purified BG505 DS-SOSIP.664	1664:1690	purified BG505 DS-SOSIP.664	1664:1690	The non-affinity purification enabled efficient scale-up, with a 250 L-scale cGMP run yielding 9.6 g of purified BG505 DS-SOSIP.664.
34020817	7	77	theme	cell	1375:1378	arg1	line					1380:1383	a CHO-DG44 stable cell line	1357:1383	a CHO-DG44 stable cell line	1357:1383	BG505 DS-SOSIP.664 was expressed in a CHO-DG44 stable cell line and purified with initial and final tangential flow filtration steps, three commercially available resin-based chromatography steps, and two orthogonal viral clearance steps.
34020817	2	78	dep	glycoprotein	245:256	arg1	trimer					258:263	trimer	258:263	The HIV-1 envelope (Env) glycoprotein trimer	220:263	The HIV-1 envelope (Env) glycoprotein trimer is covered by N-linked glycan comprising half its mass and requires both trimer assembly and subunit cleavage to fold into a prefusion-closed conformation.
34020817	10	79	theme	Env	1955:1957	arg1	RnS-3mut-2G-SOSIP.664					1996:2016	ConC-FP8v2 RnS-3mut-2G-SOSIP.664	1985:2016	ConC-FP8v2 RnS-3mut-2G-SOSIP.664	1985:2016	The developed manufacturing process was suitable for 50 L-scale production of a second prefusion-stabilized Env trimer vaccine candidate, ConC-FP8v2 RnS-3mut-2G-SOSIP.664, yielding 7.8 g of this consensus clade C trimer.
34020817	10	79	theme	Env	1955:1957	arg1	candidate					1974:1982	a second prefusion-stabilized Env trimer vaccine candidate	1925:1982	a second prefusion-stabilized Env trimer vaccine candidate	1925:1982	The developed manufacturing process was suitable for 50 L-scale production of a second prefusion-stabilized Env trimer vaccine candidate, ConC-FP8v2 RnS-3mut-2G-SOSIP.664, yielding 7.8 g of this consensus clade C trimer.
34020817	11	80	theme	successful	2072:2081	arg1	development					2091:2101	successful process development	2072:2101	successful process development	2072:2101	The successful process development and purification scale-up of HIV-1 Env trimers from different clades by using commercially available materials provide experimental demonstration for cGMP manufacturing of trimeric HIV-Env vaccine immunogens, in an antigenically desired conformation, without the use of costly affinity resins.
34020817	8	81	theme	DS-SOSIP.664	1679:1690	arg1	9.6 g					1655:1659	9.6 g	1655:1659	9.6 g of purified BG505 DS-SOSIP.664	1655:1690	The non-affinity purification enabled efficient scale-up, with a 250 L-scale cGMP run yielding 9.6 g of purified BG505 DS-SOSIP.664.
34020817	7	82	theme	CHO-DG44	1359:1366	arg1	line					1380:1383	a CHO-DG44 stable cell line	1357:1383	a CHO-DG44 stable cell line	1357:1383	BG505 DS-SOSIP.664 was expressed in a CHO-DG44 stable cell line and purified with initial and final tangential flow filtration steps, three commercially available resin-based chromatography steps, and two orthogonal viral clearance steps.
34020817	11	83	theme	costly	2373:2378	arg1	resins					2389:2394	costly affinity resins	2373:2394	costly affinity resins	2373:2394	The successful process development and purification scale-up of HIV-1 Env trimers from different clades by using commercially available materials provide experimental demonstration for cGMP manufacturing of trimeric HIV-Env vaccine immunogens, in an antigenically desired conformation, without the use of costly affinity resins.
34020817	2	84	gly	glycoprotein	245:256	arg1	glycoprotein					245:256	The HIV-1 envelope (Env) glycoprotein	220:256	The HIV-1 envelope (Env) glycoprotein trimer	220:263	The HIV-1 envelope (Env) glycoprotein trimer is covered by N-linked glycan comprising half its mass and requires both trimer assembly and subunit cleavage to fold into a prefusion-closed conformation.
34020817	5	85	theme	scalable	825:832	arg1	process					848:854	a scalable manufacturing process	823:854	a scalable manufacturing process based on commercially available, non-affinity resins	823:907	Here, we develop a scalable manufacturing process based on commercially available, non-affinity resins, and we apply the process to current GMP (cGMP) production of trimers from clades A and C, which have been found to boost cross-clade neutralizing responses in vaccine-test species.
34020817	6	86	contain	contained	1148:1156	arg2	201C-433C					1183:1191	201C-433C	1183:1191	201C-433C	1183:1191	The clade A trimer, which we named "BG505 DS-SOSIP.664", contained an engineered disulfide (201C-433C; DS) within gp120, which further stabilized this trimer in a prefusion-closed conformation resistant to CD4-induced triggering.
34020817	6	86	contain	contained	1148:1156	arg2	disulfide					1172:1180	an engineered disulfide	1158:1180	an engineered disulfide (201C-433C; DS)	1158:1196	The clade A trimer, which we named "BG505 DS-SOSIP.664", contained an engineered disulfide (201C-433C; DS) within gp120, which further stabilized this trimer in a prefusion-closed conformation resistant to CD4-induced triggering.
34020817	6	86	contain	contained	1148:1156	arg1	clade					1095:1099	The clade A trimer	1091:1108	The clade A trimer	1091:1108	The clade A trimer, which we named "BG505 DS-SOSIP.664", contained an engineered disulfide (201C-433C; DS) within gp120, which further stabilized this trimer in a prefusion-closed conformation resistant to CD4-induced triggering.
34020817	5	87	theme	cross-clade	1031:1041	arg1	responses					1056:1064	cross-clade neutralizing responses	1031:1064	cross-clade neutralizing responses in vaccine-test species	1031:1088	Here, we develop a scalable manufacturing process based on commercially available, non-affinity resins, and we apply the process to current GMP (cGMP) production of trimers from clades A and C, which have been found to boost cross-clade neutralizing responses in vaccine-test species.
34020817	11	88	theme	trimers	2142:2148	arg1	development					2091:2101	successful process development	2072:2101	successful process development	2072:2101	The successful process development and purification scale-up of HIV-1 Env trimers from different clades by using commercially available materials provide experimental demonstration for cGMP manufacturing of trimeric HIV-Env vaccine immunogens, in an antigenically desired conformation, without the use of costly affinity resins.
34020817	11	88	theme	trimers	2142:2148	arg1	scale-up					2120:2127	purification scale-up	2107:2127	purification scale-up	2107:2127	The successful process development and purification scale-up of HIV-1 Env trimers from different clades by using commercially available materials provide experimental demonstration for cGMP manufacturing of trimeric HIV-Env vaccine immunogens, in an antigenically desired conformation, without the use of costly affinity resins.
34020817	2	89	dep	half	306:309	arg1	mass					315:318	its mass	311:318	half its mass	306:318	The HIV-1 envelope (Env) glycoprotein trimer is covered by N-linked glycan comprising half its mass and requires both trimer assembly and subunit cleavage to fold into a prefusion-closed conformation.
34020817	10	90	theme	developed	1851:1859	arg1	suitable					1887:1894	suitable	1887:1894	suitable	1887:1894	The developed manufacturing process was suitable for 50 L-scale production of a second prefusion-stabilized Env trimer vaccine candidate, ConC-FP8v2 RnS-3mut-2G-SOSIP.664, yielding 7.8 g of this consensus clade C trimer.
34020817	10	90	theme	developed	1851:1859	arg1	process					1875:1881	The developed manufacturing process	1847:1881	The developed manufacturing process	1847:1881	The developed manufacturing process was suitable for 50 L-scale production of a second prefusion-stabilized Env trimer vaccine candidate, ConC-FP8v2 RnS-3mut-2G-SOSIP.664, yielding 7.8 g of this consensus clade C trimer.
34020817	11	91	theme	HIV-1	2132:2136	arg1	trimers					2142:2148	HIV-1 Env trimers	2132:2148	HIV-1 Env trimers from different clades	2132:2170	The successful process development and purification scale-up of HIV-1 Env trimers from different clades by using commercially available materials provide experimental demonstration for cGMP manufacturing of trimeric HIV-Env vaccine immunogens, in an antigenically desired conformation, without the use of costly affinity resins.
34020817	0	92	from	clades	103:108	arg1	trimers					90:96	prefusion-closed HIV-1 envelope trimers	58:96	prefusion-closed HIV-1 envelope trimers from clades A and C	58:116	A non-affinity purification process for GMP production of prefusion-closed HIV-1 envelope trimers from clades A and C for clinical evaluation.
34020817	0	92	from	clades	103:108	arg1	production					44:53	GMP production	40:53	GMP production of prefusion-closed HIV-1 envelope trimers from clades A and C	40:116	A non-affinity purification process for GMP production of prefusion-closed HIV-1 envelope trimers from clades A and C for clinical evaluation.
34020817	5	93	theme	GMP	946:948	arg1	production					957:966	current GMP (cGMP) production	938:966	current GMP (cGMP) production	938:966	Here, we develop a scalable manufacturing process based on commercially available, non-affinity resins, and we apply the process to current GMP (cGMP) production of trimers from clades A and C, which have been found to boost cross-clade neutralizing responses in vaccine-test species.
34020817	10	94	theme	C	2058:2058	arg1	trimer					2060:2065	this consensus clade C trimer	2037:2065	this consensus clade C trimer	2037:2065	The developed manufacturing process was suitable for 50 L-scale production of a second prefusion-stabilized Env trimer vaccine candidate, ConC-FP8v2 RnS-3mut-2G-SOSIP.664, yielding 7.8 g of this consensus clade C trimer.
34020817	0	95	theme	HIV-1	75:79	arg1	trimers					90:96	prefusion-closed HIV-1 envelope trimers	58:96	prefusion-closed HIV-1 envelope trimers from clades A and C	58:116	A non-affinity purification process for GMP production of prefusion-closed HIV-1 envelope trimers from clades A and C for clinical evaluation.
34020817	5	96	theme	available	878:886	arg1	resins					902:907	commercially available, non-affinity resins	865:907	commercially available, non-affinity resins	865:907	Here, we develop a scalable manufacturing process based on commercially available, non-affinity resins, and we apply the process to current GMP (cGMP) production of trimers from clades A and C, which have been found to boost cross-clade neutralizing responses in vaccine-test species.
34020817	7	97	theme	viral	1537:1541	arg1	steps					1553:1557	two orthogonal viral clearance steps	1522:1557	two orthogonal viral clearance steps	1522:1557	BG505 DS-SOSIP.664 was expressed in a CHO-DG44 stable cell line and purified with initial and final tangential flow filtration steps, three commercially available resin-based chromatography steps, and two orthogonal viral clearance steps.
34020817	11	98	theme	HIV-Env	2284:2290	arg1	immunogens					2300:2309	trimeric HIV-Env vaccine immunogens	2275:2309	trimeric HIV-Env vaccine immunogens	2275:2309	The successful process development and purification scale-up of HIV-1 Env trimers from different clades by using commercially available materials provide experimental demonstration for cGMP manufacturing of trimeric HIV-Env vaccine immunogens, in an antigenically desired conformation, without the use of costly affinity resins.
34020817	8	99	theme	non-affinity	1564:1575	arg1	purification					1577:1588	The non-affinity purification	1560:1588	The non-affinity purification	1560:1588	The non-affinity purification enabled efficient scale-up, with a 250 L-scale cGMP run yielding 9.6 g of purified BG505 DS-SOSIP.664.
34020817	10	100	theme	consensus	2042:2050	arg1	trimer					2060:2065	this consensus clade C trimer	2037:2065	this consensus clade C trimer	2037:2065	The developed manufacturing process was suitable for 50 L-scale production of a second prefusion-stabilized Env trimer vaccine candidate, ConC-FP8v2 RnS-3mut-2G-SOSIP.664, yielding 7.8 g of this consensus clade C trimer.
34020817	0	101	theme	trimers	90:96	arg1	production					44:53	GMP production	40:53	GMP production of prefusion-closed HIV-1 envelope trimers from clades A and C	40:116	A non-affinity purification process for GMP production of prefusion-closed HIV-1 envelope trimers from clades A and C for clinical evaluation.
34020817	1	102	theme	glycosylated	154:165	arg1	immunogens					167:176	Metastable glycosylated immunogens	143:176	Metastable glycosylated immunogens	143:176	Metastable glycosylated immunogens present challenges for GMP manufacturing.
34020817	9	103	theme	conformation	1754:1765	arg1	retention					1722:1730	retention	1722:1730	retention of a prefusion-closed conformation	1722:1765	Antigenic analysis indicated retention of a prefusion-closed conformation, including recognition by apex-directed and fusion peptide-directed antibodies.
34020817	4	104	dep	employed	701:708	arg1	recognizes					757:766	recognizes	757:766	recognizes only ~30% of circulating HIV strains	757:803	Prior reported GMP manufacturing for a soluble trimer stabilized in a near-native state by disulfide (SOS) and Ile-to-Pro (IP) mutations has employed affinity methods based on antibody 2G12, which recognizes only ~30% of circulating HIV strains.
34020817	11	105	theme	desired	2332:2338	arg1	conformation					2340:2351	an antigenically desired conformation	2315:2351	an antigenically desired conformation	2315:2351	The successful process development and purification scale-up of HIV-1 Env trimers from different clades by using commercially available materials provide experimental demonstration for cGMP manufacturing of trimeric HIV-Env vaccine immunogens, in an antigenically desired conformation, without the use of costly affinity resins.
34020817	11	106	from	scale-up	2120:2127	arg1	clades					2165:2170	different clades	2155:2170	different clades	2155:2170	The successful process development and purification scale-up of HIV-1 Env trimers from different clades by using commercially available materials provide experimental demonstration for cGMP manufacturing of trimeric HIV-Env vaccine immunogens, in an antigenically desired conformation, without the use of costly affinity resins.
34020817	0	107	dep	clades	103:108	arg1	clades					103:108	clades A and C	103:116	clades A and C	103:116	A non-affinity purification process for GMP production of prefusion-closed HIV-1 envelope trimers from clades A and C for clinical evaluation.
34020817	0	107	dep	clades	103:108	arg1	C					116:116	C	116:116	C	116:116	A non-affinity purification process for GMP production of prefusion-closed HIV-1 envelope trimers from clades A and C for clinical evaluation.
34020817	0	107	dep	clades	103:108	arg1	A					110:110	A	110:110	A	110:110	A non-affinity purification process for GMP production of prefusion-closed HIV-1 envelope trimers from clades A and C for clinical evaluation.
34020817	3	108	theme	antigenic	460:468	arg1	state					470:474	the vaccine-desired antigenic state	440:474	the vaccine-desired antigenic state	440:474	This conformation, the vaccine-desired antigenic state, is both metastable to structural rearrangement and labile to subunit dissociation.
34020817	3	108	theme	antigenic	460:468	arg1	conformation					426:437	This conformation	421:437	This conformation	421:437	This conformation, the vaccine-desired antigenic state, is both metastable to structural rearrangement and labile to subunit dissociation.
34020817	0	109	theme	non-affinity	2:13	arg1	process					28:34	A non-affinity purification process	0:34	A non-affinity purification process for GMP production of prefusion-closed HIV-1 envelope trimers from clades A and C for clinical evaluation.	0:141	A non-affinity purification process for GMP production of prefusion-closed HIV-1 envelope trimers from clades A and C for clinical evaluation.
34020817	5	110	theme	vaccine-test	1069:1080	arg1	species					1082:1088	vaccine-test species	1069:1088	vaccine-test species	1069:1088	Here, we develop a scalable manufacturing process based on commercially available, non-affinity resins, and we apply the process to current GMP (cGMP) production of trimers from clades A and C, which have been found to boost cross-clade neutralizing responses in vaccine-test species.
34020817	4	111	theme	strains	797:803	arg1	strains					797:803	circulating HIV strains	781:803	circulating HIV strains	781:803	Prior reported GMP manufacturing for a soluble trimer stabilized in a near-native state by disulfide (SOS) and Ile-to-Pro (IP) mutations has employed affinity methods based on antibody 2G12, which recognizes only ~30% of circulating HIV strains.
34020817	4	111	theme	strains	797:803	arg1	%					776:776	only ~30%	768:776	only ~30% of circulating HIV strains	768:803	Prior reported GMP manufacturing for a soluble trimer stabilized in a near-native state by disulfide (SOS) and Ile-to-Pro (IP) mutations has employed affinity methods based on antibody 2G12, which recognizes only ~30% of circulating HIV strains.
34020817	9	112	theme	peptide-directed	1818:1833	arg1	antibodies					1835:1844	apex-directed and fusion peptide-directed antibodies	1793:1844	apex-directed and fusion peptide-directed antibodies	1793:1844	Antigenic analysis indicated retention of a prefusion-closed conformation, including recognition by apex-directed and fusion peptide-directed antibodies.
34020817	0	113	theme	GMP	40:42	arg1	production					44:53	GMP production	40:53	GMP production of prefusion-closed HIV-1 envelope trimers from clades A and C	40:116	A non-affinity purification process for GMP production of prefusion-closed HIV-1 envelope trimers from clades A and C for clinical evaluation.
34020817	3	114	theme	vaccine-desired	444:458	arg1	state					470:474	the vaccine-desired antigenic state	440:474	the vaccine-desired antigenic state	440:474	This conformation, the vaccine-desired antigenic state, is both metastable to structural rearrangement and labile to subunit dissociation.
34020817	3	114	theme	vaccine-desired	444:458	arg1	conformation					426:437	This conformation	421:437	This conformation	421:437	This conformation, the vaccine-desired antigenic state, is both metastable to structural rearrangement and labile to subunit dissociation.
34020817	7	115	theme	resin-based	1484:1494	arg1	steps					1511:1515	three commercially available resin-based chromatography steps	1455:1515	three commercially available resin-based chromatography steps	1455:1515	BG505 DS-SOSIP.664 was expressed in a CHO-DG44 stable cell line and purified with initial and final tangential flow filtration steps, three commercially available resin-based chromatography steps, and two orthogonal viral clearance steps.
34020817	4	116	theme	reported	566:573	arg1	manufacturing					579:591	Prior reported GMP manufacturing	560:591	Prior reported GMP manufacturing for a soluble trimer stabilized in a near-native state by disulfide (SOS) and Ile-to-Pro (IP) mutations	560:695	Prior reported GMP manufacturing for a soluble trimer stabilized in a near-native state by disulfide (SOS) and Ile-to-Pro (IP) mutations has employed affinity methods based on antibody 2G12, which recognizes only ~30% of circulating HIV strains.
34020817	2	117	theme	HIV-1	224:228	arg1	glycoprotein					245:256	The HIV-1 envelope (Env) glycoprotein	220:256	The HIV-1 envelope (Env) glycoprotein trimer	220:263	The HIV-1 envelope (Env) glycoprotein trimer is covered by N-linked glycan comprising half its mass and requires both trimer assembly and subunit cleavage to fold into a prefusion-closed conformation.
34020817	2	118	theme	trimer	338:343	arg1	assembly					345:352	trimer assembly	338:352	trimer assembly	338:352	The HIV-1 envelope (Env) glycoprotein trimer is covered by N-linked glycan comprising half its mass and requires both trimer assembly and subunit cleavage to fold into a prefusion-closed conformation.
34020817	8	119	theme	efficient	1598:1606	arg1	scale-up					1608:1615	efficient scale-up	1598:1615	efficient scale-up	1598:1615	The non-affinity purification enabled efficient scale-up, with a 250 L-scale cGMP run yielding 9.6 g of purified BG505 DS-SOSIP.664.
34020817	4	120	theme	near-native	630:640	arg1	state					642:646	a near-native state	628:646	a near-native state	628:646	Prior reported GMP manufacturing for a soluble trimer stabilized in a near-native state by disulfide (SOS) and Ile-to-Pro (IP) mutations has employed affinity methods based on antibody 2G12, which recognizes only ~30% of circulating HIV strains.
34020817	10	121	theme	50	1900:1901	arg1	L-scale					1903:1909	L-scale	1903:1909	L-scale	1903:1909	The developed manufacturing process was suitable for 50 L-scale production of a second prefusion-stabilized Env trimer vaccine candidate, ConC-FP8v2 RnS-3mut-2G-SOSIP.664, yielding 7.8 g of this consensus clade C trimer.
34020817	8	122	theme	cGMP	1637:1640	arg1	run					1642:1644	a 250 L-scale cGMP run	1623:1644	a 250 L-scale cGMP run yielding 9.6 g of purified BG505 DS-SOSIP.664	1623:1690	The non-affinity purification enabled efficient scale-up, with a 250 L-scale cGMP run yielding 9.6 g of purified BG505 DS-SOSIP.664.
34020817	5	123	theme	trimers	971:977	arg1	production					957:966	current GMP (cGMP) production	938:966	current GMP (cGMP) production	938:966	Here, we develop a scalable manufacturing process based on commercially available, non-affinity resins, and we apply the process to current GMP (cGMP) production of trimers from clades A and C, which have been found to boost cross-clade neutralizing responses in vaccine-test species.
34020817	10	124	theme	prefusion-stabilized	1934:1953	arg1	RnS-3mut-2G-SOSIP.664					1996:2016	ConC-FP8v2 RnS-3mut-2G-SOSIP.664	1985:2016	ConC-FP8v2 RnS-3mut-2G-SOSIP.664	1985:2016	The developed manufacturing process was suitable for 50 L-scale production of a second prefusion-stabilized Env trimer vaccine candidate, ConC-FP8v2 RnS-3mut-2G-SOSIP.664, yielding 7.8 g of this consensus clade C trimer.
34020817	10	124	theme	prefusion-stabilized	1934:1953	arg1	candidate					1974:1982	a second prefusion-stabilized Env trimer vaccine candidate	1925:1982	a second prefusion-stabilized Env trimer vaccine candidate	1925:1982	The developed manufacturing process was suitable for 50 L-scale production of a second prefusion-stabilized Env trimer vaccine candidate, ConC-FP8v2 RnS-3mut-2G-SOSIP.664, yielding 7.8 g of this consensus clade C trimer.
34020817	4	125	theme	HIV	793:795	arg1	strains					797:803	circulating HIV strains	781:803	circulating HIV strains	781:803	Prior reported GMP manufacturing for a soluble trimer stabilized in a near-native state by disulfide (SOS) and Ile-to-Pro (IP) mutations has employed affinity methods based on antibody 2G12, which recognizes only ~30% of circulating HIV strains.
34020817	3	126	theme	subunit	538:544	arg1	dissociation					546:557	subunit dissociation	538:557	subunit dissociation	538:557	This conformation, the vaccine-desired antigenic state, is both metastable to structural rearrangement and labile to subunit dissociation.
34556431	8	0	from	Patients	1173:1180	arg1	group					1193:1197	the USG group	1185:1197	the USG group	1185:1197	Patients in the USG group had a significantly lower incidence of vascular complications compared with patients in the FSG group (8.5% vs. 21.0%, p = 0.039), driven by a reduction of localized hematomas (3.4% vs 13.0%, p = 0.042).
34556431	9	1	theme	primary	1542:1548	arg1	outcome					1550:1556	the composite primary outcome	1528:1556	the composite primary outcome	1528:1556	After adjustment for type of CTO approach and heparin dose, USG was significantly associated with a reduced relative risk of the composite primary outcome (adjusted odds ratio 0.16, 95% confidence interval 0.05 to 0.51; p = 0.002).
34556431	1	2	theme	total	280:284	arg1	CTO					298:300	CTO	298:300	CTO	298:300	OBJECTIVE To compare vascular complications in patients undergoing percutaneous coronary intervention (PCI) of chronic total occlusions (CTO) using ultrasound guidance (USG) versus fluoroscopy guidance (FSG) for femoral access.
34556431	1	2	theme	total	280:284	arg1	occlusions					286:295	chronic total occlusions	272:295	chronic total occlusions (CTO) using ultrasound guidance (USG) versus fluoroscopy guidance (FSG)	272:367	OBJECTIVE To compare vascular complications in patients undergoing percutaneous coronary intervention (PCI) of chronic total occlusions (CTO) using ultrasound guidance (USG) versus fluoroscopy guidance (FSG) for femoral access.
34556431	10	3	from	reduction	1746:1754	arg1	hematomas					1765:1773	local hematomas	1759:1773	local hematomas	1759:1773	CONCLUSION USG in CTO-PCI is associated with a decreased risk of vascular complications, primarily driven by a reduction in local hematomas, especially in complex CTO-PCI where the larger use of heparin increases the risk of vascular complications.
34556431	10	4	theme	heparin	1830:1836	arg1	use					1823:1825	the larger use	1812:1825	the larger use of heparin	1812:1836	CONCLUSION USG in CTO-PCI is associated with a decreased risk of vascular complications, primarily driven by a reduction in local hematomas, especially in complex CTO-PCI where the larger use of heparin increases the risk of vascular complications.
34556431	9	5	theme	95	1585:1586	arg1	%					1587:1587	%	1587:1587	%	1587:1587	After adjustment for type of CTO approach and heparin dose, USG was significantly associated with a reduced relative risk of the composite primary outcome (adjusted odds ratio 0.16, 95% confidence interval 0.05 to 0.51; p = 0.002).
34556431	8	6	theme	vascular	1238:1245	arg1	complications					1247:1259	vascular complications	1238:1259	vascular complications	1238:1259	Patients in the USG group had a significantly lower incidence of vascular complications compared with patients in the FSG group (8.5% vs. 21.0%, p = 0.039), driven by a reduction of localized hematomas (3.4% vs 13.0%, p = 0.042).
34556431	7	7	dep	RESULTS	1114:1120	arg1	occurred					1142:1149	occurred	1142:1149	occurred in 17.3% of patients	1142:1170	RESULTS The primary outcome occurred in 17.3% of patients.
34556431	0	8	theme	FOUND	135:139	arg1	Registry					151:158	FOUND BLOOD CTO Registry	135:158	FOUND BLOOD CTO Registry	135:158	Ultrasound- Versus Fluoroscopy-Guided Femoral Access for Percutaneous Coronary Intervention of Chronic Total Occlusions: Insights From FOUND BLOOD CTO Registry.
34556431	1	9	theme	percutaneous	228:239	arg1	PCI					264:266	PCI	264:266	PCI	264:266	OBJECTIVE To compare vascular complications in patients undergoing percutaneous coronary intervention (PCI) of chronic total occlusions (CTO) using ultrasound guidance (USG) versus fluoroscopy guidance (FSG) for femoral access.
34556431	1	9	theme	percutaneous	228:239	arg1	intervention					250:261	percutaneous coronary intervention	228:261	percutaneous coronary intervention (PCI) of chronic total occlusions (CTO) using ultrasound guidance (USG) versus fluoroscopy guidance (FSG) for femoral access	228:386	OBJECTIVE To compare vascular complications in patients undergoing percutaneous coronary intervention (PCI) of chronic total occlusions (CTO) using ultrasound guidance (USG) versus fluoroscopy guidance (FSG) for femoral access.
34556431	8	10	theme	USG	1189:1191	arg1	group					1193:1197	the USG group	1185:1197	the USG group	1185:1197	Patients in the USG group had a significantly lower incidence of vascular complications compared with patients in the FSG group (8.5% vs. 21.0%, p = 0.039), driven by a reduction of localized hematomas (3.4% vs 13.0%, p = 0.042).
34556431	9	11	theme	confidence	1589:1598	arg1	interval					1600:1607	95% confidence interval 0.05 to 0.51	1585:1620	95% confidence interval 0.05 to 0.51	1585:1620	After adjustment for type of CTO approach and heparin dose, USG was significantly associated with a reduced relative risk of the composite primary outcome (adjusted odds ratio 0.16, 95% confidence interval 0.05 to 0.51; p = 0.002).
34556431	9	11	theme	confidence	1589:1598	arg1	odds					1568:1571	adjusted odds ratio 0.16	1559:1582	adjusted odds ratio 0.16	1559:1582	After adjustment for type of CTO approach and heparin dose, USG was significantly associated with a reduced relative risk of the composite primary outcome (adjusted odds ratio 0.16, 95% confidence interval 0.05 to 0.51; p = 0.002).
34556431	0	12	theme	CTO	147:149	arg1	Registry					151:158	FOUND BLOOD CTO Registry	135:158	FOUND BLOOD CTO Registry	135:158	Ultrasound- Versus Fluoroscopy-Guided Femoral Access for Percutaneous Coronary Intervention of Chronic Total Occlusions: Insights From FOUND BLOOD CTO Registry.
34556431	4	13	dep	November	805:812	arg1	to					819:820	to	819:820	to	819:820	METHODS A total of 197 patients undergoing CTO-PCI using at least a femoral vascular access from November 2015 to September 2020 were screened.
34556431	4	13	dep	November	805:812	arg1	September					822:830	September	822:830	September	822:830	METHODS A total of 197 patients undergoing CTO-PCI using at least a femoral vascular access from November 2015 to September 2020 were screened.
34556431	8	14	dep	%	1305:1305	arg1	p = 0.039					1318:1326	p = 0.039	1318:1326	p = 0.039	1318:1326	Patients in the USG group had a significantly lower incidence of vascular complications compared with patients in the FSG group (8.5% vs. 21.0%, p = 0.039), driven by a reduction of localized hematomas (3.4% vs 13.0%, p = 0.042).
34556431	3	15	theme	attempts	579:586	arg1	complications					602:614	vascular complications	593:614	vascular complications compared with FSG	593:632	USG reduces time to access, number of attempts, and vascular complications compared with FSG, but the efficacy of USG has never been tested in the setting of CTO-PCI.
34556431	3	15	theme	attempts	579:586	arg1	time					553:556	time	553:556	time to access	553:566	USG reduces time to access, number of attempts, and vascular complications compared with FSG, but the efficacy of USG has never been tested in the setting of CTO-PCI.
34556431	3	15	theme	attempts	579:586	arg1	number					569:574	number	569:574	number of attempts	569:586	USG reduces time to access, number of attempts, and vascular complications compared with FSG, but the efficacy of USG has never been tested in the setting of CTO-PCI.
34556431	10	16	theme	vascular	1700:1707	arg1	complications					1709:1721	vascular complications	1700:1721	vascular complications	1700:1721	CONCLUSION USG in CTO-PCI is associated with a decreased risk of vascular complications, primarily driven by a reduction in local hematomas, especially in complex CTO-PCI where the larger use of heparin increases the risk of vascular complications.
34556431	10	17	theme	complications	1869:1881	arg1	risk					1852:1855	the risk	1848:1855	the risk of vascular complications	1848:1881	CONCLUSION USG in CTO-PCI is associated with a decreased risk of vascular complications, primarily driven by a reduction in local hematomas, especially in complex CTO-PCI where the larger use of heparin increases the risk of vascular complications.
34556431	9	18	dep	interval	1600:1607	arg1	to					1614:1615	to	1614:1615	to	1614:1615	After adjustment for type of CTO approach and heparin dose, USG was significantly associated with a reduced relative risk of the composite primary outcome (adjusted odds ratio 0.16, 95% confidence interval 0.05 to 0.51; p = 0.002).
34556431	2	19	theme	vascular	484:491	arg1	complications					493:505	vascular complications	484:505	vascular complications	484:505	BACKGROUND In patients undergoing PCI, using the arterial femoral access increases the risk of vascular complications compared using the radial access.
34556431	0	20	theme	Chronic	95:101	arg1	Occlusions					109:118	Chronic Total Occlusions	95:118	Chronic Total Occlusions	95:118	Ultrasound- Versus Fluoroscopy-Guided Femoral Access for Percutaneous Coronary Intervention of Chronic Total Occlusions: Insights From FOUND BLOOD CTO Registry.
34556431	9	21	theme	approach	1436:1443	arg1	type					1424:1427	type	1424:1427	type of CTO approach and heparin dose	1424:1460	After adjustment for type of CTO approach and heparin dose, USG was significantly associated with a reduced relative risk of the composite primary outcome (adjusted odds ratio 0.16, 95% confidence interval 0.05 to 0.51; p = 0.002).
34556431	0	22	theme	Occlusions	109:118	arg1	Intervention					79:90	Percutaneous Coronary Intervention	57:90	Percutaneous Coronary Intervention of Chronic Total Occlusions	57:118	Ultrasound- Versus Fluoroscopy-Guided Femoral Access for Percutaneous Coronary Intervention of Chronic Total Occlusions: Insights From FOUND BLOOD CTO Registry.
34556431	8	23	from	patients	1275:1282	arg1	group					1295:1299	the FSG group	1287:1299	the FSG group (8.5% vs. 21.0%, p = 0.039)	1287:1327	Patients in the USG group had a significantly lower incidence of vascular complications compared with patients in the FSG group (8.5% vs. 21.0%, p = 0.039), driven by a reduction of localized hematomas (3.4% vs 13.0%, p = 0.042).
34556431	8	23	from	patients	1275:1282	arg1	%					1305:1305	8.5%	1302:1305	8.5%	1302:1305	Patients in the USG group had a significantly lower incidence of vascular complications compared with patients in the FSG group (8.5% vs. 21.0%, p = 0.039), driven by a reduction of localized hematomas (3.4% vs 13.0%, p = 0.042).
34556431	8	23	from	patients	1275:1282	arg1	%					1315:1315	21.0%	1311:1315	21.0%	1311:1315	Patients in the USG group had a significantly lower incidence of vascular complications compared with patients in the FSG group (8.5% vs. 21.0%, p = 0.039), driven by a reduction of localized hematomas (3.4% vs 13.0%, p = 0.042).
34556431	9	24	theme	heparin	1449:1455	arg1	dose					1457:1460	heparin dose	1449:1460	heparin dose	1449:1460	After adjustment for type of CTO approach and heparin dose, USG was significantly associated with a reduced relative risk of the composite primary outcome (adjusted odds ratio 0.16, 95% confidence interval 0.05 to 0.51; p = 0.002).
34556431	2	25	theme	femoral	447:453	arg1	access					455:460	the arterial femoral access	434:460	the arterial femoral access	434:460	BACKGROUND In patients undergoing PCI, using the arterial femoral access increases the risk of vascular complications compared using the radial access.
34556431	0	26	theme	Ultrasound-	0:10	arg1	Access					46:51	Ultrasound- Versus Fluoroscopy-Guided Femoral Access	0:51	Ultrasound- Versus Fluoroscopy-Guided Femoral Access for Percutaneous Coronary Intervention of Chronic Total Occlusions: Insights From FOUND BLOOD CTO Registry.	0:159	Ultrasound- Versus Fluoroscopy-Guided Femoral Access for Percutaneous Coronary Intervention of Chronic Total Occlusions: Insights From FOUND BLOOD CTO Registry.
34556431	5	27	theme	hematoma	897:904	arg1	composite					878:886	a composite	876:886	a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL	876:998	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	27	theme	hematoma	897:904	arg1	hemorrhage					939:948	retroperitoneal hemorrhage	923:948	retroperitoneal hemorrhage	923:948	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	27	theme	hematoma	897:904	arg1	≥3 g/dL					992:998	hemoglobin drop ≥3 g/dL	976:998	hemoglobin drop ≥3 g/dL	976:998	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	27	theme	hematoma	897:904	arg1	outcome					864:870	The primary outcome	852:870	The primary outcome	852:870	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	27	theme	hematoma	897:904	arg1	hematoma					897:904	local hematoma	891:904	local hematoma	891:904	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	27	theme	hematoma	897:904	arg1	pseudoaneurysm					907:920	pseudoaneurysm	907:920	pseudoaneurysm	907:920	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	27	theme	hematoma	897:904	arg1	fistula					965:971	arteriovenous fistula	951:971	arteriovenous fistula	951:971	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	8	28	dep	had	1199:1201	arg1	%					1379:1379	3.4%	1376:1379	3.4%	1376:1379	Patients in the USG group had a significantly lower incidence of vascular complications compared with patients in the FSG group (8.5% vs. 21.0%, p = 0.039), driven by a reduction of localized hematomas (3.4% vs 13.0%, p = 0.042).
34556431	8	28	dep	had	1199:1201	arg1	%					1388:1388	13.0%	1384:1388	13.0%	1384:1388	Patients in the USG group had a significantly lower incidence of vascular complications compared with patients in the FSG group (8.5% vs. 21.0%, p = 0.039), driven by a reduction of localized hematomas (3.4% vs 13.0%, p = 0.042).
34556431	0	29	theme	Fluoroscopy-Guided	19:36	arg1	Access					46:51	Ultrasound- Versus Fluoroscopy-Guided Femoral Access	0:51	Ultrasound- Versus Fluoroscopy-Guided Femoral Access for Percutaneous Coronary Intervention of Chronic Total Occlusions: Insights From FOUND BLOOD CTO Registry.	0:159	Ultrasound- Versus Fluoroscopy-Guided Femoral Access for Percutaneous Coronary Intervention of Chronic Total Occlusions: Insights From FOUND BLOOD CTO Registry.
34556431	5	30	theme	pseudoaneurysm	907:920	arg1	composite					878:886	a composite	876:886	a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL	876:998	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	30	theme	pseudoaneurysm	907:920	arg1	hemorrhage					939:948	retroperitoneal hemorrhage	923:948	retroperitoneal hemorrhage	923:948	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	30	theme	pseudoaneurysm	907:920	arg1	≥3 g/dL					992:998	hemoglobin drop ≥3 g/dL	976:998	hemoglobin drop ≥3 g/dL	976:998	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	30	theme	pseudoaneurysm	907:920	arg1	outcome					864:870	The primary outcome	852:870	The primary outcome	852:870	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	30	theme	pseudoaneurysm	907:920	arg1	hematoma					897:904	local hematoma	891:904	local hematoma	891:904	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	30	theme	pseudoaneurysm	907:920	arg1	pseudoaneurysm					907:920	pseudoaneurysm	907:920	pseudoaneurysm	907:920	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	30	theme	pseudoaneurysm	907:920	arg1	fistula					965:971	arteriovenous fistula	951:971	arteriovenous fistula	951:971	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	6	31	theme	independent	1028:1038	arg1	association					1040:1050	The independent association	1024:1050	The independent association between USG	1024:1062	The independent association between USG and the primary outcome of interest was explored.
34556431	4	32	theme	femoral	776:782	arg1	access					793:798	a femoral vascular access	774:798	at least a femoral vascular access	765:798	METHODS A total of 197 patients undergoing CTO-PCI using at least a femoral vascular access from November 2015 to September 2020 were screened.
34556431	5	33	theme	≥3 g/dL	992:998	arg1	composite					878:886	a composite	876:886	a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL	876:998	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	33	theme	≥3 g/dL	992:998	arg1	hemorrhage					939:948	retroperitoneal hemorrhage	923:948	retroperitoneal hemorrhage	923:948	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	33	theme	≥3 g/dL	992:998	arg1	≥3 g/dL					992:998	hemoglobin drop ≥3 g/dL	976:998	hemoglobin drop ≥3 g/dL	976:998	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	33	theme	≥3 g/dL	992:998	arg1	outcome					864:870	The primary outcome	852:870	The primary outcome	852:870	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	33	theme	≥3 g/dL	992:998	arg1	hematoma					897:904	local hematoma	891:904	local hematoma	891:904	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	33	theme	≥3 g/dL	992:998	arg1	pseudoaneurysm					907:920	pseudoaneurysm	907:920	pseudoaneurysm	907:920	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	33	theme	≥3 g/dL	992:998	arg1	fistula					965:971	arteriovenous fistula	951:971	arteriovenous fistula	951:971	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	7	34	theme	primary	1126:1132	arg1	outcome					1134:1140	The primary outcome	1122:1140	The primary outcome	1122:1140	RESULTS The primary outcome occurred in 17.3% of patients.
34556431	8	35	dep	%	1379:1379	arg1	p = 0.042					1391:1399	p = 0.042	1391:1399	p = 0.042	1391:1399	Patients in the USG group had a significantly lower incidence of vascular complications compared with patients in the FSG group (8.5% vs. 21.0%, p = 0.039), driven by a reduction of localized hematomas (3.4% vs 13.0%, p = 0.042).
34556431	9	36	theme	relative	1511:1518	arg1	risk					1520:1523	a reduced relative risk	1501:1523	a reduced relative risk of the composite primary outcome	1501:1556	After adjustment for type of CTO approach and heparin dose, USG was significantly associated with a reduced relative risk of the composite primary outcome (adjusted odds ratio 0.16, 95% confidence interval 0.05 to 0.51; p = 0.002).
34556431	8	37	theme	localized	1355:1363	arg1	hematomas					1365:1373	localized hematomas	1355:1373	localized hematomas	1355:1373	Patients in the USG group had a significantly lower incidence of vascular complications compared with patients in the FSG group (8.5% vs. 21.0%, p = 0.039), driven by a reduction of localized hematomas (3.4% vs 13.0%, p = 0.042).
34556431	5	38	theme	fistula	965:971	arg1	composite					878:886	a composite	876:886	a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL	876:998	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	38	theme	fistula	965:971	arg1	hemorrhage					939:948	retroperitoneal hemorrhage	923:948	retroperitoneal hemorrhage	923:948	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	38	theme	fistula	965:971	arg1	≥3 g/dL					992:998	hemoglobin drop ≥3 g/dL	976:998	hemoglobin drop ≥3 g/dL	976:998	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	38	theme	fistula	965:971	arg1	outcome					864:870	The primary outcome	852:870	The primary outcome	852:870	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	38	theme	fistula	965:971	arg1	hematoma					897:904	local hematoma	891:904	local hematoma	891:904	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	38	theme	fistula	965:971	arg1	pseudoaneurysm					907:920	pseudoaneurysm	907:920	pseudoaneurysm	907:920	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	38	theme	fistula	965:971	arg1	fistula					965:971	arteriovenous fistula	951:971	arteriovenous fistula	951:971	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	8	39	theme	FSG	1291:1293	arg1	group					1295:1299	the FSG group	1287:1299	the FSG group (8.5% vs. 21.0%, p = 0.039)	1287:1327	Patients in the USG group had a significantly lower incidence of vascular complications compared with patients in the FSG group (8.5% vs. 21.0%, p = 0.039), driven by a reduction of localized hematomas (3.4% vs 13.0%, p = 0.042).
34556431	8	39	theme	FSG	1291:1293	arg1	%					1305:1305	8.5%	1302:1305	8.5%	1302:1305	Patients in the USG group had a significantly lower incidence of vascular complications compared with patients in the FSG group (8.5% vs. 21.0%, p = 0.039), driven by a reduction of localized hematomas (3.4% vs 13.0%, p = 0.042).
34556431	8	39	theme	FSG	1291:1293	arg1	%					1315:1315	21.0%	1311:1315	21.0%	1311:1315	Patients in the USG group had a significantly lower incidence of vascular complications compared with patients in the FSG group (8.5% vs. 21.0%, p = 0.039), driven by a reduction of localized hematomas (3.4% vs 13.0%, p = 0.042).
34556431	9	40	dep	odds	1568:1571	arg1	ratio					1573:1577	ratio 0.16	1573:1582	adjusted odds ratio 0.16	1559:1582	After adjustment for type of CTO approach and heparin dose, USG was significantly associated with a reduced relative risk of the composite primary outcome (adjusted odds ratio 0.16, 95% confidence interval 0.05 to 0.51; p = 0.002).
34556431	9	40	dep	odds	1568:1571	arg1	p = 0.002					1623:1631	p = 0.002	1623:1631	p = 0.002	1623:1631	After adjustment for type of CTO approach and heparin dose, USG was significantly associated with a reduced relative risk of the composite primary outcome (adjusted odds ratio 0.16, 95% confidence interval 0.05 to 0.51; p = 0.002).
34556431	9	41	theme	composite	1532:1540	arg1	outcome					1550:1556	the composite primary outcome	1528:1556	the composite primary outcome	1528:1556	After adjustment for type of CTO approach and heparin dose, USG was significantly associated with a reduced relative risk of the composite primary outcome (adjusted odds ratio 0.16, 95% confidence interval 0.05 to 0.51; p = 0.002).
34556431	1	42	theme	chronic	272:278	arg1	CTO					298:300	CTO	298:300	CTO	298:300	OBJECTIVE To compare vascular complications in patients undergoing percutaneous coronary intervention (PCI) of chronic total occlusions (CTO) using ultrasound guidance (USG) versus fluoroscopy guidance (FSG) for femoral access.
34556431	1	42	theme	chronic	272:278	arg1	occlusions					286:295	chronic total occlusions	272:295	chronic total occlusions (CTO) using ultrasound guidance (USG) versus fluoroscopy guidance (FSG)	272:367	OBJECTIVE To compare vascular complications in patients undergoing percutaneous coronary intervention (PCI) of chronic total occlusions (CTO) using ultrasound guidance (USG) versus fluoroscopy guidance (FSG) for femoral access.
34556431	9	43	theme	outcome	1550:1556	arg1	risk					1520:1523	a reduced relative risk	1501:1523	a reduced relative risk of the composite primary outcome	1501:1556	After adjustment for type of CTO approach and heparin dose, USG was significantly associated with a reduced relative risk of the composite primary outcome (adjusted odds ratio 0.16, 95% confidence interval 0.05 to 0.51; p = 0.002).
34556431	6	44	theme	primary	1072:1078	arg1	outcome					1080:1086	the primary outcome	1068:1086	the primary outcome of interest	1068:1098	The independent association between USG and the primary outcome of interest was explored.
34556431	10	45	theme	CONCLUSION	1635:1644	arg1	USG					1646:1648	CONCLUSION USG	1635:1648	CONCLUSION USG in CTO-PCI	1635:1659	CONCLUSION USG in CTO-PCI is associated with a decreased risk of vascular complications, primarily driven by a reduction in local hematomas, especially in complex CTO-PCI where the larger use of heparin increases the risk of vascular complications.
34556431	1	46	theme	occlusions	286:295	arg1	PCI					264:266	PCI	264:266	PCI	264:266	OBJECTIVE To compare vascular complications in patients undergoing percutaneous coronary intervention (PCI) of chronic total occlusions (CTO) using ultrasound guidance (USG) versus fluoroscopy guidance (FSG) for femoral access.
34556431	1	46	theme	occlusions	286:295	arg1	intervention					250:261	percutaneous coronary intervention	228:261	percutaneous coronary intervention (PCI) of chronic total occlusions (CTO) using ultrasound guidance (USG) versus fluoroscopy guidance (FSG) for femoral access	228:386	OBJECTIVE To compare vascular complications in patients undergoing percutaneous coronary intervention (PCI) of chronic total occlusions (CTO) using ultrasound guidance (USG) versus fluoroscopy guidance (FSG) for femoral access.
34556431	9	47	theme	adjusted	1559:1566	arg1	interval					1600:1607	95% confidence interval 0.05 to 0.51	1585:1620	95% confidence interval 0.05 to 0.51	1585:1620	After adjustment for type of CTO approach and heparin dose, USG was significantly associated with a reduced relative risk of the composite primary outcome (adjusted odds ratio 0.16, 95% confidence interval 0.05 to 0.51; p = 0.002).
34556431	9	47	theme	adjusted	1559:1566	arg1	odds					1568:1571	adjusted odds ratio 0.16	1559:1582	adjusted odds ratio 0.16	1559:1582	After adjustment for type of CTO approach and heparin dose, USG was significantly associated with a reduced relative risk of the composite primary outcome (adjusted odds ratio 0.16, 95% confidence interval 0.05 to 0.51; p = 0.002).
34556431	9	48	theme	CTO	1432:1434	arg1	approach					1436:1443	CTO approach	1432:1443	CTO approach	1432:1443	After adjustment for type of CTO approach and heparin dose, USG was significantly associated with a reduced relative risk of the composite primary outcome (adjusted odds ratio 0.16, 95% confidence interval 0.05 to 0.51; p = 0.002).
34556431	3	49	theme	CTO-PCI	699:705	arg1	setting					688:694	the setting	684:694	the setting of CTO-PCI	684:705	USG reduces time to access, number of attempts, and vascular complications compared with FSG, but the efficacy of USG has never been tested in the setting of CTO-PCI.
34556431	1	50	theme	vascular	182:189	arg1	complications					191:203	vascular complications	182:203	vascular complications in patients undergoing percutaneous coronary intervention (PCI) of chronic total occlusions (CTO) using ultrasound guidance (USG) versus fluoroscopy guidance (FSG) for femoral access	182:386	OBJECTIVE To compare vascular complications in patients undergoing percutaneous coronary intervention (PCI) of chronic total occlusions (CTO) using ultrasound guidance (USG) versus fluoroscopy guidance (FSG) for femoral access.
34556431	0	51	theme	Percutaneous	57:68	arg1	Intervention					79:90	Percutaneous Coronary Intervention	57:90	Percutaneous Coronary Intervention of Chronic Total Occlusions	57:118	Ultrasound- Versus Fluoroscopy-Guided Femoral Access for Percutaneous Coronary Intervention of Chronic Total Occlusions: Insights From FOUND BLOOD CTO Registry.
34556431	10	52	theme	complex	1790:1796	arg1	CTO-PCI					1798:1804	complex CTO-PCI	1790:1804	complex CTO-PCI where the larger use of heparin increases the risk of vascular complications	1790:1881	CONCLUSION USG in CTO-PCI is associated with a decreased risk of vascular complications, primarily driven by a reduction in local hematomas, especially in complex CTO-PCI where the larger use of heparin increases the risk of vascular complications.
34556431	10	53	from	hematomas	1765:1773	arg1	CTO-PCI					1798:1804	complex CTO-PCI	1790:1804	complex CTO-PCI where the larger use of heparin increases the risk of vascular complications	1790:1881	CONCLUSION USG in CTO-PCI is associated with a decreased risk of vascular complications, primarily driven by a reduction in local hematomas, especially in complex CTO-PCI where the larger use of heparin increases the risk of vascular complications.
34556431	10	54	theme	local	1759:1763	arg1	hematomas					1765:1773	local hematomas	1759:1773	local hematomas	1759:1773	CONCLUSION USG in CTO-PCI is associated with a decreased risk of vascular complications, primarily driven by a reduction in local hematomas, especially in complex CTO-PCI where the larger use of heparin increases the risk of vascular complications.
34556431	8	55	theme	lower	1219:1223	arg1	incidence					1225:1233	a significantly lower incidence	1203:1233	a significantly lower incidence of vascular complications	1203:1259	Patients in the USG group had a significantly lower incidence of vascular complications compared with patients in the FSG group (8.5% vs. 21.0%, p = 0.039), driven by a reduction of localized hematomas (3.4% vs 13.0%, p = 0.042).
34556431	0	56	theme	BLOOD	141:145	arg1	Registry					151:158	FOUND BLOOD CTO Registry	135:158	FOUND BLOOD CTO Registry	135:158	Ultrasound- Versus Fluoroscopy-Guided Femoral Access for Percutaneous Coronary Intervention of Chronic Total Occlusions: Insights From FOUND BLOOD CTO Registry.
34556431	0	57	dep	Access	46:51	arg1	Insights					121:128	Insights	121:128	Ultrasound- Versus Fluoroscopy-Guided Femoral Access for Percutaneous Coronary Intervention of Chronic Total Occlusions: Insights From FOUND BLOOD CTO Registry.	0:159	Ultrasound- Versus Fluoroscopy-Guided Femoral Access for Percutaneous Coronary Intervention of Chronic Total Occlusions: Insights From FOUND BLOOD CTO Registry.
34556431	9	58	theme	%	1587:1587	arg1	interval					1600:1607	95% confidence interval 0.05 to 0.51	1585:1620	95% confidence interval 0.05 to 0.51	1585:1620	After adjustment for type of CTO approach and heparin dose, USG was significantly associated with a reduced relative risk of the composite primary outcome (adjusted odds ratio 0.16, 95% confidence interval 0.05 to 0.51; p = 0.002).
34556431	9	58	theme	%	1587:1587	arg1	odds					1568:1571	adjusted odds ratio 0.16	1559:1582	adjusted odds ratio 0.16	1559:1582	After adjustment for type of CTO approach and heparin dose, USG was significantly associated with a reduced relative risk of the composite primary outcome (adjusted odds ratio 0.16, 95% confidence interval 0.05 to 0.51; p = 0.002).
34556431	10	59	theme	larger	1816:1821	arg1	use					1823:1825	the larger use	1812:1825	the larger use of heparin	1812:1836	CONCLUSION USG in CTO-PCI is associated with a decreased risk of vascular complications, primarily driven by a reduction in local hematomas, especially in complex CTO-PCI where the larger use of heparin increases the risk of vascular complications.
34556431	10	60	theme	decreased	1682:1690	arg1	risk					1692:1695	a decreased risk	1680:1695	a decreased risk of vascular complications, primarily driven by a reduction in local hematomas, especially in complex CTO-PCI where the larger use of heparin increases the risk of vascular complications	1680:1881	CONCLUSION USG in CTO-PCI is associated with a decreased risk of vascular complications, primarily driven by a reduction in local hematomas, especially in complex CTO-PCI where the larger use of heparin increases the risk of vascular complications.
34556431	1	61	theme	coronary	241:248	arg1	PCI					264:266	PCI	264:266	PCI	264:266	OBJECTIVE To compare vascular complications in patients undergoing percutaneous coronary intervention (PCI) of chronic total occlusions (CTO) using ultrasound guidance (USG) versus fluoroscopy guidance (FSG) for femoral access.
34556431	1	61	theme	coronary	241:248	arg1	intervention					250:261	percutaneous coronary intervention	228:261	percutaneous coronary intervention (PCI) of chronic total occlusions (CTO) using ultrasound guidance (USG) versus fluoroscopy guidance (FSG) for femoral access	228:386	OBJECTIVE To compare vascular complications in patients undergoing percutaneous coronary intervention (PCI) of chronic total occlusions (CTO) using ultrasound guidance (USG) versus fluoroscopy guidance (FSG) for femoral access.
34556431	8	62	theme	complications	1247:1259	arg1	incidence					1225:1233	a significantly lower incidence	1203:1233	a significantly lower incidence of vascular complications	1203:1259	Patients in the USG group had a significantly lower incidence of vascular complications compared with patients in the FSG group (8.5% vs. 21.0%, p = 0.039), driven by a reduction of localized hematomas (3.4% vs 13.0%, p = 0.042).
34556431	10	63	theme	complications	1709:1721	arg1	risk					1692:1695	a decreased risk	1680:1695	a decreased risk of vascular complications, primarily driven by a reduction in local hematomas, especially in complex CTO-PCI where the larger use of heparin increases the risk of vascular complications	1680:1881	CONCLUSION USG in CTO-PCI is associated with a decreased risk of vascular complications, primarily driven by a reduction in local hematomas, especially in complex CTO-PCI where the larger use of heparin increases the risk of vascular complications.
34556431	4	64	dep	METHODS	708:714	arg1	total					718:722	A total	716:722	METHODS A total of 197 patients undergoing CTO-PCI using at least a femoral vascular access from November 2015 to September 2020	708:835	METHODS A total of 197 patients undergoing CTO-PCI using at least a femoral vascular access from November 2015 to September 2020 were screened.
34556431	2	65	from	BACKGROUND	389:398	arg1	patients					403:410	patients	403:410	patients undergoing PCI	403:425	BACKGROUND In patients undergoing PCI, using the arterial femoral access increases the risk of vascular complications compared using the radial access.
34556431	3	66	theme	vascular	593:600	arg1	complications					602:614	vascular complications	593:614	vascular complications compared with FSG	593:632	USG reduces time to access, number of attempts, and vascular complications compared with FSG, but the efficacy of USG has never been tested in the setting of CTO-PCI.
34556431	2	67	theme	complications	493:505	arg1	risk					476:479	the risk	472:479	the risk of vascular complications compared using the radial access	472:538	BACKGROUND In patients undergoing PCI, using the arterial femoral access increases the risk of vascular complications compared using the radial access.
34556431	9	68	dep	associated	1485:1494	arg1	interval					1600:1607	95% confidence interval 0.05 to 0.51	1585:1620	95% confidence interval 0.05 to 0.51	1585:1620	After adjustment for type of CTO approach and heparin dose, USG was significantly associated with a reduced relative risk of the composite primary outcome (adjusted odds ratio 0.16, 95% confidence interval 0.05 to 0.51; p = 0.002).
34556431	9	68	dep	associated	1485:1494	arg1	odds					1568:1571	adjusted odds ratio 0.16	1559:1582	adjusted odds ratio 0.16	1559:1582	After adjustment for type of CTO approach and heparin dose, USG was significantly associated with a reduced relative risk of the composite primary outcome (adjusted odds ratio 0.16, 95% confidence interval 0.05 to 0.51; p = 0.002).
34556431	10	69	theme	vascular	1860:1867	arg1	complications					1869:1881	vascular complications	1860:1881	vascular complications	1860:1881	CONCLUSION USG in CTO-PCI is associated with a decreased risk of vascular complications, primarily driven by a reduction in local hematomas, especially in complex CTO-PCI where the larger use of heparin increases the risk of vascular complications.
34556431	0	70	theme	Total	103:107	arg1	Occlusions					109:118	Chronic Total Occlusions	95:118	Chronic Total Occlusions	95:118	Ultrasound- Versus Fluoroscopy-Guided Femoral Access for Percutaneous Coronary Intervention of Chronic Total Occlusions: Insights From FOUND BLOOD CTO Registry.
34556431	1	71	theme	fluoroscopy	342:352	arg1	guidance					354:361	fluoroscopy guidance	342:361	fluoroscopy guidance (FSG)	342:367	OBJECTIVE To compare vascular complications in patients undergoing percutaneous coronary intervention (PCI) of chronic total occlusions (CTO) using ultrasound guidance (USG) versus fluoroscopy guidance (FSG) for femoral access.
34556431	1	71	theme	fluoroscopy	342:352	arg1	FSG					364:366	FSG	364:366	FSG	364:366	OBJECTIVE To compare vascular complications in patients undergoing percutaneous coronary intervention (PCI) of chronic total occlusions (CTO) using ultrasound guidance (USG) versus fluoroscopy guidance (FSG) for femoral access.
34556431	1	72	from	complications	191:203	arg1	patients					208:215	patients	208:215	patients undergoing percutaneous coronary intervention (PCI) of chronic total occlusions (CTO) using ultrasound guidance (USG) versus fluoroscopy guidance (FSG) for femoral access	208:386	OBJECTIVE To compare vascular complications in patients undergoing percutaneous coronary intervention (PCI) of chronic total occlusions (CTO) using ultrasound guidance (USG) versus fluoroscopy guidance (FSG) for femoral access.
34556431	5	73	theme	primary	856:862	arg1	composite					878:886	a composite	876:886	a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL	876:998	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	73	theme	primary	856:862	arg1	hemorrhage					939:948	retroperitoneal hemorrhage	923:948	retroperitoneal hemorrhage	923:948	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	73	theme	primary	856:862	arg1	≥3 g/dL					992:998	hemoglobin drop ≥3 g/dL	976:998	hemoglobin drop ≥3 g/dL	976:998	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	73	theme	primary	856:862	arg1	outcome					864:870	The primary outcome	852:870	The primary outcome	852:870	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	73	theme	primary	856:862	arg1	hematoma					897:904	local hematoma	891:904	local hematoma	891:904	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	73	theme	primary	856:862	arg1	pseudoaneurysm					907:920	pseudoaneurysm	907:920	pseudoaneurysm	907:920	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	73	theme	primary	856:862	arg1	fistula					965:971	arteriovenous fistula	951:971	arteriovenous fistula	951:971	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	10	74	from	USG	1646:1648	arg1	CTO-PCI					1653:1659	CTO-PCI	1653:1659	CTO-PCI	1653:1659	CONCLUSION USG in CTO-PCI is associated with a decreased risk of vascular complications, primarily driven by a reduction in local hematomas, especially in complex CTO-PCI where the larger use of heparin increases the risk of vascular complications.
34556431	2	75	theme	radial	526:531	arg1	access					533:538	the radial access	522:538	the radial access	522:538	BACKGROUND In patients undergoing PCI, using the arterial femoral access increases the risk of vascular complications compared using the radial access.
34556431	5	76	theme	retroperitoneal	923:937	arg1	hemorrhage					939:948	retroperitoneal hemorrhage	923:948	retroperitoneal hemorrhage	923:948	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	7	77	theme	patients	1163:1170	arg1	patients					1163:1170	patients	1163:1170	patients	1163:1170	RESULTS The primary outcome occurred in 17.3% of patients.
34556431	7	77	theme	patients	1163:1170	arg1	%					1158:1158	17.3%	1154:1158	17.3% of patients	1154:1170	RESULTS The primary outcome occurred in 17.3% of patients.
34556431	9	78	theme	dose	1457:1460	arg1	type					1424:1427	type	1424:1427	type of CTO approach and heparin dose	1424:1460	After adjustment for type of CTO approach and heparin dose, USG was significantly associated with a reduced relative risk of the composite primary outcome (adjusted odds ratio 0.16, 95% confidence interval 0.05 to 0.51; p = 0.002).
34556431	4	79	theme	patients	731:738	arg1	total					718:722	A total	716:722	METHODS A total of 197 patients undergoing CTO-PCI using at least a femoral vascular access from November 2015 to September 2020	708:835	METHODS A total of 197 patients undergoing CTO-PCI using at least a femoral vascular access from November 2015 to September 2020 were screened.
34556431	1	80	theme	femoral	373:379	arg1	access					381:386	femoral access	373:386	femoral access	373:386	OBJECTIVE To compare vascular complications in patients undergoing percutaneous coronary intervention (PCI) of chronic total occlusions (CTO) using ultrasound guidance (USG) versus fluoroscopy guidance (FSG) for femoral access.
34556431	5	81	theme	local	891:895	arg1	hematoma					897:904	local hematoma	891:904	local hematoma	891:904	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	2	82	theme	arterial	438:445	arg1	access					455:460	the arterial femoral access	434:460	the arterial femoral access	434:460	BACKGROUND In patients undergoing PCI, using the arterial femoral access increases the risk of vascular complications compared using the radial access.
34556431	0	83	theme	Femoral	38:44	arg1	Access					46:51	Ultrasound- Versus Fluoroscopy-Guided Femoral Access	0:51	Ultrasound- Versus Fluoroscopy-Guided Femoral Access for Percutaneous Coronary Intervention of Chronic Total Occlusions: Insights From FOUND BLOOD CTO Registry.	0:159	Ultrasound- Versus Fluoroscopy-Guided Femoral Access for Percutaneous Coronary Intervention of Chronic Total Occlusions: Insights From FOUND BLOOD CTO Registry.
34556431	5	84	theme	hemorrhage	939:948	arg1	composite					878:886	a composite	876:886	a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL	876:998	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	84	theme	hemorrhage	939:948	arg1	hemorrhage					939:948	retroperitoneal hemorrhage	923:948	retroperitoneal hemorrhage	923:948	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	84	theme	hemorrhage	939:948	arg1	≥3 g/dL					992:998	hemoglobin drop ≥3 g/dL	976:998	hemoglobin drop ≥3 g/dL	976:998	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	84	theme	hemorrhage	939:948	arg1	outcome					864:870	The primary outcome	852:870	The primary outcome	852:870	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	84	theme	hemorrhage	939:948	arg1	hematoma					897:904	local hematoma	891:904	local hematoma	891:904	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	84	theme	hemorrhage	939:948	arg1	pseudoaneurysm					907:920	pseudoaneurysm	907:920	pseudoaneurysm	907:920	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	84	theme	hemorrhage	939:948	arg1	fistula					965:971	arteriovenous fistula	951:971	arteriovenous fistula	951:971	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	1	85	theme	ultrasound	309:318	arg1	USG					330:332	USG	330:332	USG	330:332	OBJECTIVE To compare vascular complications in patients undergoing percutaneous coronary intervention (PCI) of chronic total occlusions (CTO) using ultrasound guidance (USG) versus fluoroscopy guidance (FSG) for femoral access.
34556431	1	85	theme	ultrasound	309:318	arg1	guidance					320:327	ultrasound guidance	309:327	ultrasound guidance (USG)	309:333	OBJECTIVE To compare vascular complications in patients undergoing percutaneous coronary intervention (PCI) of chronic total occlusions (CTO) using ultrasound guidance (USG) versus fluoroscopy guidance (FSG) for femoral access.
34556431	5	86	theme	drop	987:990	arg1	≥3 g/dL					992:998	hemoglobin drop ≥3 g/dL	976:998	hemoglobin drop ≥3 g/dL	976:998	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	0	87	theme	Coronary	70:77	arg1	Intervention					79:90	Percutaneous Coronary Intervention	57:90	Percutaneous Coronary Intervention of Chronic Total Occlusions	57:118	Ultrasound- Versus Fluoroscopy-Guided Femoral Access for Percutaneous Coronary Intervention of Chronic Total Occlusions: Insights From FOUND BLOOD CTO Registry.
34556431	9	88	theme	reduced	1503:1509	arg1	risk					1520:1523	a reduced relative risk	1501:1523	a reduced relative risk of the composite primary outcome	1501:1556	After adjustment for type of CTO approach and heparin dose, USG was significantly associated with a reduced relative risk of the composite primary outcome (adjusted odds ratio 0.16, 95% confidence interval 0.05 to 0.51; p = 0.002).
34556431	3	89	theme	USG	655:657	arg1	efficacy					643:650	the efficacy	639:650	the efficacy of USG	639:657	USG reduces time to access, number of attempts, and vascular complications compared with FSG, but the efficacy of USG has never been tested in the setting of CTO-PCI.
34556431	4	90	theme	vascular	784:791	arg1	access					793:798	a femoral vascular access	774:798	at least a femoral vascular access	765:798	METHODS A total of 197 patients undergoing CTO-PCI using at least a femoral vascular access from November 2015 to September 2020 were screened.
34556431	5	91	theme	hemoglobin	976:985	arg1	≥3 g/dL					992:998	hemoglobin drop ≥3 g/dL	976:998	hemoglobin drop ≥3 g/dL	976:998	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	5	92	theme	arteriovenous	951:963	arg1	fistula					965:971	arteriovenous fistula	951:971	arteriovenous fistula	951:971	The primary outcome was a composite of local hematoma, pseudoaneurysm, retroperitoneal hemorrhage, arteriovenous fistula or hemoglobin drop ≥3 g/dL during hospitalization.
34556431	8	93	contain	had	1199:1201	arg1	Patients					1173:1180	Patients	1173:1180	Patients in the USG group	1173:1197	Patients in the USG group had a significantly lower incidence of vascular complications compared with patients in the FSG group (8.5% vs. 21.0%, p = 0.039), driven by a reduction of localized hematomas (3.4% vs 13.0%, p = 0.042).
34556431	8	93	contain	had	1199:1201	arg2	incidence					1225:1233	a significantly lower incidence	1203:1233	a significantly lower incidence of vascular complications	1203:1259	Patients in the USG group had a significantly lower incidence of vascular complications compared with patients in the FSG group (8.5% vs. 21.0%, p = 0.039), driven by a reduction of localized hematomas (3.4% vs 13.0%, p = 0.042).
34556431	0	94	from	Registry	151:158	arg1	Insights					121:128	Insights	121:128	Ultrasound- Versus Fluoroscopy-Guided Femoral Access for Percutaneous Coronary Intervention of Chronic Total Occlusions: Insights From FOUND BLOOD CTO Registry.	0:159	Ultrasound- Versus Fluoroscopy-Guided Femoral Access for Percutaneous Coronary Intervention of Chronic Total Occlusions: Insights From FOUND BLOOD CTO Registry.
34556431	6	95	theme	interest	1091:1098	arg1	association					1040:1050	The independent association	1024:1050	The independent association between USG	1024:1062	The independent association between USG and the primary outcome of interest was explored.
34556431	6	95	theme	interest	1091:1098	arg1	outcome					1080:1086	the primary outcome	1068:1086	the primary outcome of interest	1068:1098	The independent association between USG and the primary outcome of interest was explored.
34556431	8	96	theme	hematomas	1365:1373	arg1	reduction					1342:1350	a reduction	1340:1350	a reduction of localized hematomas	1340:1373	Patients in the USG group had a significantly lower incidence of vascular complications compared with patients in the FSG group (8.5% vs. 21.0%, p = 0.039), driven by a reduction of localized hematomas (3.4% vs 13.0%, p = 0.042).
32032821	0	0	theme	parviflora	95:104	arg1	fingerprints					48:59	HPLC fingerprints	43:59	HPLC fingerprints	43:59	Spectrum-effect relationship study between HPLC fingerprints and antioxidant activity of Sabia parviflora.
32032821	0	0	theme	parviflora	95:104	arg1	activity					77:84	antioxidant activity	65:84	antioxidant activity of Sabia parviflora	65:104	Spectrum-effect relationship study between HPLC fingerprints and antioxidant activity of Sabia parviflora.
32032821	4	1	theme	active	512:517	arg1	substances					519:528	the antioxidant active substances	496:528	the antioxidant active substances	496:528	And the antioxidant active substances were selected by spectrum-effect relationship.
32032821	1	2	theme	human	178:182	arg1	worldwide					191:199	human health worldwide	178:199	human health worldwide	178:199	Nowadays, the increased prevalence of sub-health significantly affects human health worldwide.
32032821	0	3	theme	Sabia	89:93	arg1	parviflora					95:104	Sabia parviflora	89:104	Sabia parviflora	89:104	Spectrum-effect relationship study between HPLC fingerprints and antioxidant activity of Sabia parviflora.
32032821	8	4	theme	sub-healthy	1145:1155	arg1	population					1157:1166	sub-healthy population	1145:1166	sub-healthy population	1145:1166	With therapeutic potential for sub-health, this special tea might provide dietotherapy/herbal to remedy sub-healthy population.
32032821	7	5	theme	potential	1017:1025	arg1	antioxidants					1027:1038	potential antioxidants	1017:1038	potential antioxidants	1017:1038	These findings revealed that the abundant flavonoids in Sabia parviflora had significant antioxidant activity and could be potential antioxidants.
32032821	1	6	theme	health	184:189	arg1	worldwide					191:199	human health worldwide	178:199	human health worldwide	178:199	Nowadays, the increased prevalence of sub-health significantly affects human health worldwide.
32032821	3	7	theme	antioxidant	381:391	arg1	activity					393:400	the fingerprint and antioxidant activity	361:400	the fingerprint and antioxidant activity of Sabia parviflora	361:420	In this research, the fingerprint and antioxidant activity of Sabia parviflora were obtained by HPLC instrument and DPPH, ABTS, FRAP heatmap assays.
32032821	5	8	theme	EE	693:694	arg1	extracts					696:703	EW, EE extracts	689:703	EW, EE extracts	689:703	The results showed that significant differences in chemical compositions of samples from different sources, and EW, EE extracts had strong antioxidant activity.
32032821	6	9	theme	promoting	855:863	arg1	flavonoids					843:852	various flavonoids	835:852	various flavonoids (promoting, competing or antagonizing)	835:891	The antioxidant activity of Sabia parviflora was mainly determined by the complex interaction of various flavonoids (promoting, competing or antagonizing).
32032821	5	10	theme	samples	653:659	arg1	compositions					637:648	chemical compositions	628:648	chemical compositions of samples from different sources	628:682	The results showed that significant differences in chemical compositions of samples from different sources, and EW, EE extracts had strong antioxidant activity.
32032821	5	11	from	compositions	637:648	arg1	sources					676:682	different sources	666:682	different sources	666:682	The results showed that significant differences in chemical compositions of samples from different sources, and EW, EE extracts had strong antioxidant activity.
32032821	5	12	from	differences	613:623	arg1	compositions					637:648	chemical compositions	628:648	chemical compositions of samples from different sources	628:682	The results showed that significant differences in chemical compositions of samples from different sources, and EW, EE extracts had strong antioxidant activity.
32032821	3	13	theme	fingerprint	365:375	arg1	activity					393:400	the fingerprint and antioxidant activity	361:400	the fingerprint and antioxidant activity of Sabia parviflora	361:420	In this research, the fingerprint and antioxidant activity of Sabia parviflora were obtained by HPLC instrument and DPPH, ABTS, FRAP heatmap assays.
32032821	8	14	theme	therapeutic	1046:1056	arg1	potential					1058:1066	therapeutic potential	1046:1066	therapeutic potential for sub-health	1046:1081	With therapeutic potential for sub-health, this special tea might provide dietotherapy/herbal to remedy sub-healthy population.
32032821	5	15	theme	different	666:674	arg1	sources					676:682	different sources	666:682	different sources	666:682	The results showed that significant differences in chemical compositions of samples from different sources, and EW, EE extracts had strong antioxidant activity.
32032821	6	16	theme	flavonoids	843:852	arg1	interaction					820:830	the complex interaction	808:830	the complex interaction of various flavonoids (promoting, competing or antagonizing)	808:891	The antioxidant activity of Sabia parviflora was mainly determined by the complex interaction of various flavonoids (promoting, competing or antagonizing).
32032821	6	17	theme	antioxidant	742:752	arg1	activity					754:761	The antioxidant activity	738:761	The antioxidant activity of Sabia parviflora	738:781	The antioxidant activity of Sabia parviflora was mainly determined by the complex interaction of various flavonoids (promoting, competing or antagonizing).
32032821	4	18	theme	antioxidant	500:510	arg1	substances					519:528	the antioxidant active substances	496:528	the antioxidant active substances	496:528	And the antioxidant active substances were selected by spectrum-effect relationship.
32032821	5	19	theme	significant	601:611	arg1	differences					613:623	that significant differences	596:623	that significant differences in chemical compositions of samples from different sources	596:682	The results showed that significant differences in chemical compositions of samples from different sources, and EW, EE extracts had strong antioxidant activity.
32032821	7	20	from	flavonoids	936:945	arg1	parviflora					956:965	Sabia parviflora	950:965	Sabia parviflora	950:965	These findings revealed that the abundant flavonoids in Sabia parviflora had significant antioxidant activity and could be potential antioxidants.
32032821	3	21	theme	parviflora	411:420	arg1	activity					393:400	the fingerprint and antioxidant activity	361:400	the fingerprint and antioxidant activity of Sabia parviflora	361:420	In this research, the fingerprint and antioxidant activity of Sabia parviflora were obtained by HPLC instrument and DPPH, ABTS, FRAP heatmap assays.
32032821	7	22	contain	had	967:969	arg1	flavonoids					936:945	the abundant flavonoids	923:945	the abundant flavonoids in Sabia parviflora	923:965	These findings revealed that the abundant flavonoids in Sabia parviflora had significant antioxidant activity and could be potential antioxidants.
32032821	7	22	contain	had	967:969	arg2	activity					995:1002	significant antioxidant activity	971:1002	significant antioxidant activity	971:1002	These findings revealed that the abundant flavonoids in Sabia parviflora had significant antioxidant activity and could be potential antioxidants.
32032821	7	23	theme	antioxidant	983:993	arg1	activity					995:1002	significant antioxidant activity	971:1002	significant antioxidant activity	971:1002	These findings revealed that the abundant flavonoids in Sabia parviflora had significant antioxidant activity and could be potential antioxidants.
32032821	0	24	theme	Spectrum-effect	0:14	arg1	relationship					16:27	Spectrum-effect relationship	0:27	Spectrum-effect relationship	0:27	Spectrum-effect relationship study between HPLC fingerprints and antioxidant activity of Sabia parviflora.
32032821	7	25	theme	significant	971:981	arg1	activity					995:1002	significant antioxidant activity	971:1002	significant antioxidant activity	971:1002	These findings revealed that the abundant flavonoids in Sabia parviflora had significant antioxidant activity and could be potential antioxidants.
32032821	8	26	theme	special	1089:1095	arg1	tea					1097:1099	this special tea	1084:1099	this special tea	1084:1099	With therapeutic potential for sub-health, this special tea might provide dietotherapy/herbal to remedy sub-healthy population.
32032821	5	27	theme	chemical	628:635	arg1	compositions					637:648	chemical compositions	628:648	chemical compositions of samples from different sources	628:682	The results showed that significant differences in chemical compositions of samples from different sources, and EW, EE extracts had strong antioxidant activity.
32032821	3	28	theme	heatmap	476:482	arg1	instrument					444:453	HPLC instrument	439:453	HPLC instrument	439:453	In this research, the fingerprint and antioxidant activity of Sabia parviflora were obtained by HPLC instrument and DPPH, ABTS, FRAP heatmap assays.
32032821	3	28	theme	heatmap	476:482	arg1	assays					484:489	FRAP heatmap assays	471:489	FRAP heatmap assays	471:489	In this research, the fingerprint and antioxidant activity of Sabia parviflora were obtained by HPLC instrument and DPPH, ABTS, FRAP heatmap assays.
32032821	3	29	theme	Sabia	405:409	arg1	parviflora					411:420	Sabia parviflora	405:420	Sabia parviflora	405:420	In this research, the fingerprint and antioxidant activity of Sabia parviflora were obtained by HPLC instrument and DPPH, ABTS, FRAP heatmap assays.
32032821	2	30	theme	effective	328:336	arg1	way					338:340	an effective way	325:340	an effective way	325:340	Suppressed sub-healthy by dietotherapy/herbal remedy which showed excellent safety profile, low cost and effectiveness, is an effective way.
32032821	2	30	theme	effective	328:336	arg1	sub-healthy					213:223	Suppressed sub-healthy	202:223	Suppressed sub-healthy by dietotherapy/herbal remedy which showed excellent safety profile, low cost and effectiveness,	202:320	Suppressed sub-healthy by dietotherapy/herbal remedy which showed excellent safety profile, low cost and effectiveness, is an effective way.
32032821	3	31	theme	HPLC	439:442	arg1	assays					484:489	FRAP heatmap assays	471:489	FRAP heatmap assays	471:489	In this research, the fingerprint and antioxidant activity of Sabia parviflora were obtained by HPLC instrument and DPPH, ABTS, FRAP heatmap assays.
32032821	3	31	theme	HPLC	439:442	arg1	ABTS					465:468	ABTS	465:468	ABTS	465:468	In this research, the fingerprint and antioxidant activity of Sabia parviflora were obtained by HPLC instrument and DPPH, ABTS, FRAP heatmap assays.
32032821	3	31	theme	HPLC	439:442	arg1	instrument					444:453	HPLC instrument	439:453	HPLC instrument	439:453	In this research, the fingerprint and antioxidant activity of Sabia parviflora were obtained by HPLC instrument and DPPH, ABTS, FRAP heatmap assays.
32032821	5	32	from	sources	676:682	arg1	samples					653:659	samples	653:659	samples from different sources	653:682	The results showed that significant differences in chemical compositions of samples from different sources, and EW, EE extracts had strong antioxidant activity.
32032821	5	32	from	sources	676:682	arg1	compositions					637:648	chemical compositions	628:648	chemical compositions of samples from different sources	628:682	The results showed that significant differences in chemical compositions of samples from different sources, and EW, EE extracts had strong antioxidant activity.
32032821	2	33	theme	Suppressed	202:211	arg1	way					338:340	an effective way	325:340	an effective way	325:340	Suppressed sub-healthy by dietotherapy/herbal remedy which showed excellent safety profile, low cost and effectiveness, is an effective way.
32032821	2	33	theme	Suppressed	202:211	arg1	sub-healthy					213:223	Suppressed sub-healthy	202:223	Suppressed sub-healthy by dietotherapy/herbal remedy which showed excellent safety profile, low cost and effectiveness,	202:320	Suppressed sub-healthy by dietotherapy/herbal remedy which showed excellent safety profile, low cost and effectiveness, is an effective way.
32032821	0	34	theme	HPLC	43:46	arg1	fingerprints					48:59	HPLC fingerprints	43:59	HPLC fingerprints	43:59	Spectrum-effect relationship study between HPLC fingerprints and antioxidant activity of Sabia parviflora.
32032821	4	35	theme	spectrum-effect	547:561	arg1	relationship					563:574	spectrum-effect relationship	547:574	spectrum-effect relationship	547:574	And the antioxidant active substances were selected by spectrum-effect relationship.
32032821	2	36	theme	safety	278:283	arg1	profile					285:291	excellent safety profile	268:291	excellent safety profile	268:291	Suppressed sub-healthy by dietotherapy/herbal remedy which showed excellent safety profile, low cost and effectiveness, is an effective way.
32032821	3	37	theme	FRAP	471:474	arg1	instrument					444:453	HPLC instrument	439:453	HPLC instrument	439:453	In this research, the fingerprint and antioxidant activity of Sabia parviflora were obtained by HPLC instrument and DPPH, ABTS, FRAP heatmap assays.
32032821	3	37	theme	FRAP	471:474	arg1	assays					484:489	FRAP heatmap assays	471:489	FRAP heatmap assays	471:489	In this research, the fingerprint and antioxidant activity of Sabia parviflora were obtained by HPLC instrument and DPPH, ABTS, FRAP heatmap assays.
32032821	0	38	theme	antioxidant	65:75	arg1	activity					77:84	antioxidant activity	65:84	antioxidant activity of Sabia parviflora	65:104	Spectrum-effect relationship study between HPLC fingerprints and antioxidant activity of Sabia parviflora.
32032821	2	39	theme	excellent	268:276	arg1	profile					285:291	excellent safety profile	268:291	excellent safety profile	268:291	Suppressed sub-healthy by dietotherapy/herbal remedy which showed excellent safety profile, low cost and effectiveness, is an effective way.
32032821	6	40	theme	complex	812:818	arg1	interaction					820:830	the complex interaction	808:830	the complex interaction of various flavonoids (promoting, competing or antagonizing)	808:891	The antioxidant activity of Sabia parviflora was mainly determined by the complex interaction of various flavonoids (promoting, competing or antagonizing).
32032821	1	41	theme	increased	121:129	arg1	Nowadays					107:114	Nowadays	107:114	Nowadays	107:114	Nowadays, the increased prevalence of sub-health significantly affects human health worldwide.
32032821	1	41	theme	increased	121:129	arg1	prevalence					131:140	the increased prevalence	117:140	the increased prevalence of sub-health	117:154	Nowadays, the increased prevalence of sub-health significantly affects human health worldwide.
32032821	5	42	theme	strong	709:714	arg1	activity					728:735	strong antioxidant activity	709:735	strong antioxidant activity	709:735	The results showed that significant differences in chemical compositions of samples from different sources, and EW, EE extracts had strong antioxidant activity.
32032821	5	43	contain	had	705:707	arg2	activity					728:735	strong antioxidant activity	709:735	strong antioxidant activity	709:735	The results showed that significant differences in chemical compositions of samples from different sources, and EW, EE extracts had strong antioxidant activity.
32032821	5	43	contain	had	705:707	arg1	extracts					696:703	EW, EE extracts	689:703	EW, EE extracts	689:703	The results showed that significant differences in chemical compositions of samples from different sources, and EW, EE extracts had strong antioxidant activity.
32032821	5	44	theme	antioxidant	716:726	arg1	activity					728:735	strong antioxidant activity	709:735	strong antioxidant activity	709:735	The results showed that significant differences in chemical compositions of samples from different sources, and EW, EE extracts had strong antioxidant activity.
32032821	6	45	theme	antagonizing	879:890	arg1	flavonoids					843:852	various flavonoids	835:852	various flavonoids (promoting, competing or antagonizing)	835:891	The antioxidant activity of Sabia parviflora was mainly determined by the complex interaction of various flavonoids (promoting, competing or antagonizing).
32032821	6	46	theme	various	835:841	arg1	flavonoids					843:852	various flavonoids	835:852	various flavonoids (promoting, competing or antagonizing)	835:891	The antioxidant activity of Sabia parviflora was mainly determined by the complex interaction of various flavonoids (promoting, competing or antagonizing).
32032821	2	47	theme	dietotherapy/herbal	228:246	arg1	cost					298:301	low cost	294:301	low cost	294:301	Suppressed sub-healthy by dietotherapy/herbal remedy which showed excellent safety profile, low cost and effectiveness, is an effective way.
32032821	2	47	theme	dietotherapy/herbal	228:246	arg1	effectiveness					307:319	effectiveness	307:319	effectiveness	307:319	Suppressed sub-healthy by dietotherapy/herbal remedy which showed excellent safety profile, low cost and effectiveness, is an effective way.
32032821	2	47	theme	dietotherapy/herbal	228:246	arg1	remedy					248:253	dietotherapy/herbal remedy	228:253	dietotherapy/herbal remedy which showed excellent safety profile	228:291	Suppressed sub-healthy by dietotherapy/herbal remedy which showed excellent safety profile, low cost and effectiveness, is an effective way.
32032821	1	48	theme	sub-health	145:154	arg1	Nowadays					107:114	Nowadays	107:114	Nowadays	107:114	Nowadays, the increased prevalence of sub-health significantly affects human health worldwide.
32032821	1	48	theme	sub-health	145:154	arg1	prevalence					131:140	the increased prevalence	117:140	the increased prevalence of sub-health	117:154	Nowadays, the increased prevalence of sub-health significantly affects human health worldwide.
32032821	6	49	theme	competing	866:874	arg1	flavonoids					843:852	various flavonoids	835:852	various flavonoids (promoting, competing or antagonizing)	835:891	The antioxidant activity of Sabia parviflora was mainly determined by the complex interaction of various flavonoids (promoting, competing or antagonizing).
32032821	2	50	theme	low	294:296	arg1	remedy					248:253	dietotherapy/herbal remedy	228:253	dietotherapy/herbal remedy which showed excellent safety profile	228:291	Suppressed sub-healthy by dietotherapy/herbal remedy which showed excellent safety profile, low cost and effectiveness, is an effective way.
32032821	2	50	theme	low	294:296	arg1	cost					298:301	low cost	294:301	low cost	294:301	Suppressed sub-healthy by dietotherapy/herbal remedy which showed excellent safety profile, low cost and effectiveness, is an effective way.
32032821	6	51	theme	parviflora	772:781	arg1	activity					754:761	The antioxidant activity	738:761	The antioxidant activity of Sabia parviflora	738:781	The antioxidant activity of Sabia parviflora was mainly determined by the complex interaction of various flavonoids (promoting, competing or antagonizing).
32032821	7	52	theme	Sabia	950:954	arg1	parviflora					956:965	Sabia parviflora	950:965	Sabia parviflora	950:965	These findings revealed that the abundant flavonoids in Sabia parviflora had significant antioxidant activity and could be potential antioxidants.
32032821	6	53	theme	Sabia	766:770	arg1	parviflora					772:781	Sabia parviflora	766:781	Sabia parviflora	766:781	The antioxidant activity of Sabia parviflora was mainly determined by the complex interaction of various flavonoids (promoting, competing or antagonizing).
32032821	7	54	theme	abundant	927:934	arg1	flavonoids					936:945	the abundant flavonoids	923:945	the abundant flavonoids in Sabia parviflora	923:965	These findings revealed that the abundant flavonoids in Sabia parviflora had significant antioxidant activity and could be potential antioxidants.
33459997	10	0	theme	controlled	1817:1826	arg1	trial					1828:1832	large-scale randomized controlled trial	1794:1832	large-scale randomized controlled trial	1794:1832	The outcomes of our research prove that multistrain live probiotic composition amplifies the positive action of CS in osteoarthritis attenuation and necessitates further investigation with large-scale randomized controlled trial.
33459997	10	1	theme	randomized	1806:1815	arg1	trial					1828:1832	large-scale randomized controlled trial	1794:1832	large-scale randomized controlled trial	1794:1832	The outcomes of our research prove that multistrain live probiotic composition amplifies the positive action of CS in osteoarthritis attenuation and necessitates further investigation with large-scale randomized controlled trial.
33459997	2	2	theme	study	354:358	arg1	aim					342:344	The aim	338:344	The aim of this study	338:358	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	1	3	theme	small	273:277	arg1	number					279:284	a small number	271:284	a small number of animal and human studies	271:312	The therapeutic potential of using probiotics to treat osteoarthritis (OA) has only recently been recognized, with a small number of animal and human studies having been undertaken.
33459997	5	4	from	expression	1040:1049	arg1	cartilage					1058:1066	the cartilage	1054:1066	the cartilage	1054:1066	Experimental OA caused an upregulation in Tlr2, Tlr4, Nfkb1, and downregulation of Comp expression in the cartilage.
33459997	10	5	theme	large-scale	1794:1804	arg1	trial					1828:1832	large-scale randomized controlled trial	1794:1832	large-scale randomized controlled trial	1794:1832	The outcomes of our research prove that multistrain live probiotic composition amplifies the positive action of CS in osteoarthritis attenuation and necessitates further investigation with large-scale randomized controlled trial.
33459997	10	6	theme	research	1625:1632	arg1	outcomes					1609:1616	The outcomes	1605:1616	The outcomes of our research	1605:1632	The outcomes of our research prove that multistrain live probiotic composition amplifies the positive action of CS in osteoarthritis attenuation and necessitates further investigation with large-scale randomized controlled trial.
33459997	8	7	theme	studied	1393:1399	arg1	markers					1401:1407	the studied markers	1389:1407	the studied markers of cartilage metabolism in serum	1389:1440	The levels of the studied markers of cartilage metabolism in serum were decreased or increased (IGF-1).
33459997	9	8	theme	combined	1483:1490	arg1	use					1492:1494	The combined use	1479:1494	The combined use of PB and CS	1479:1507	The combined use of PB and CS was more effective than separate application approaching above-mentioned parameters to control.
33459997	9	8	theme	combined	1483:1490	arg1	effective					1518:1526	effective	1518:1526	effective	1518:1526	The combined use of PB and CS was more effective than separate application approaching above-mentioned parameters to control.
33459997	6	9	theme	CTSK	1176:1179	arg1	levels					1181:1186	CTSK levels	1176:1186	CTSK levels	1176:1186	MIA-OA caused a significant increase of TLR-2 soluble form and IL-6, IL-8, TGF-β1, COMP, ACAN, CHI3L1, and CTSK levels in the blood serum; the level of IGF-1, on contrary, decreased.
33459997	6	10	theme	significant	1085:1095	arg1	increase					1097:1104	a significant increase	1083:1104	a significant increase of TLR-2 soluble form	1083:1126	MIA-OA caused a significant increase of TLR-2 soluble form and IL-6, IL-8, TGF-β1, COMP, ACAN, CHI3L1, and CTSK levels in the blood serum; the level of IGF-1, on contrary, decreased.
33459997	10	11	theme	live	1657:1660	arg1	composition					1672:1682	multistrain live probiotic composition	1645:1682	multistrain live probiotic composition	1645:1682	The outcomes of our research prove that multistrain live probiotic composition amplifies the positive action of CS in osteoarthritis attenuation and necessitates further investigation with large-scale randomized controlled trial.
33459997	8	12	theme	metabolism	1422:1431	arg1	markers					1401:1407	the studied markers	1389:1407	the studied markers of cartilage metabolism in serum	1389:1440	The levels of the studied markers of cartilage metabolism in serum were decreased or increased (IGF-1).
33459997	4	13	theme	TLR-2	885:889	arg1	levels					818:823	The levels	814:823	The levels of IL-6, IL-8, TGF-β1, IGF-1, COMP, ACAN, CHI3L1, CTSK, and TLR-2	814:889	The levels of IL-6, IL-8, TGF-β1, IGF-1, COMP, ACAN, CHI3L1, CTSK, and TLR-2 were measured in serum by enzyme-linked immunosorbent assay.
33459997	6	14	theme	soluble	1115:1121	arg1	form					1123:1126	TLR-2 soluble form	1109:1126	TLR-2 soluble form	1109:1126	MIA-OA caused a significant increase of TLR-2 soluble form and IL-6, IL-8, TGF-β1, COMP, ACAN, CHI3L1, and CTSK levels in the blood serum; the level of IGF-1, on contrary, decreased.
33459997	7	15	theme	Tlr4	1333:1336	arg1	expressions					1349:1359	reduced Tlr2, Tlr4, and Nfkb1 expressions	1319:1359	reduced Tlr2, Tlr4, and Nfkb1 expressions	1319:1359	Separate administration of PB and CS raised expression of Comp and reduced Tlr2, Tlr4, and Nfkb1 expressions in cartilage.
33459997	9	16	theme	above-mentioned	1566:1580	arg1	parameters					1582:1591	above-mentioned parameters	1566:1591	above-mentioned parameters	1566:1591	The combined use of PB and CS was more effective than separate application approaching above-mentioned parameters to control.
33459997	6	17	from	levels	1181:1186	arg1	serum					1201:1205	the blood serum	1191:1205	the blood serum	1191:1205	MIA-OA caused a significant increase of TLR-2 soluble form and IL-6, IL-8, TGF-β1, COMP, ACAN, CHI3L1, and CTSK levels in the blood serum; the level of IGF-1, on contrary, decreased.
33459997	7	18	theme	Tlr2	1327:1330	arg1	expressions					1349:1359	reduced Tlr2, Tlr4, and Nfkb1 expressions	1319:1359	reduced Tlr2, Tlr4, and Nfkb1 expressions	1319:1359	Separate administration of PB and CS raised expression of Comp and reduced Tlr2, Tlr4, and Nfkb1 expressions in cartilage.
33459997	2	19	theme	gene	523:526	arg1	expression					528:537	Comp gene expression	518:537	Comp gene expression	518:537	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	5	20	from	downregulation	1017:1030	arg1	Tlr4					1000:1003	Tlr4	1000:1003	Tlr4	1000:1003	Experimental OA caused an upregulation in Tlr2, Tlr4, Nfkb1, and downregulation of Comp expression in the cartilage.
33459997	5	20	from	downregulation	1017:1030	arg1	cartilage					1058:1066	the cartilage	1054:1066	the cartilage	1054:1066	Experimental OA caused an upregulation in Tlr2, Tlr4, Nfkb1, and downregulation of Comp expression in the cartilage.
33459997	5	20	from	downregulation	1017:1030	arg1	Tlr2					994:997	Tlr2	994:997	Tlr2	994:997	Experimental OA caused an upregulation in Tlr2, Tlr4, Nfkb1, and downregulation of Comp expression in the cartilage.
33459997	5	20	from	downregulation	1017:1030	arg1	Nfkb1					1006:1010	Nfkb1	1006:1010	Nfkb1	1006:1010	Experimental OA caused an upregulation in Tlr2, Tlr4, Nfkb1, and downregulation of Comp expression in the cartilage.
33459997	10	21	theme	CS	1717:1718	arg1	action					1707:1712	the positive action	1694:1712	the positive action of CS	1694:1718	The outcomes of our research prove that multistrain live probiotic composition amplifies the positive action of CS in osteoarthritis attenuation and necessitates further investigation with large-scale randomized controlled trial.
33459997	6	22	from	CHI3L1	1164:1169	arg1	serum					1201:1205	the blood serum	1191:1205	the blood serum	1191:1205	MIA-OA caused a significant increase of TLR-2 soluble form and IL-6, IL-8, TGF-β1, COMP, ACAN, CHI3L1, and CTSK levels in the blood serum; the level of IGF-1, on contrary, decreased.
33459997	0	23	from	Metabolism	113:122	arg1	Osteoarthritis					140:153	Experimental Osteoarthritis	127:153	Experimental Osteoarthritis	127:153	Probiotic Composition and Chondroitin Sulfate Regulate TLR-2/4-Mediated NF-κB Inflammatory Pathway and Cartilage Metabolism in Experimental Osteoarthritis.
33459997	0	24	theme	Inflammatory	78:89	arg1	Pathway					91:97	TLR-2/4-Mediated NF-κB Inflammatory Pathway	55:97	TLR-2/4-Mediated NF-κB Inflammatory Pathway	55:97	Probiotic Composition and Chondroitin Sulfate Regulate TLR-2/4-Mediated NF-κB Inflammatory Pathway and Cartilage Metabolism in Experimental Osteoarthritis.
33459997	6	25	from	TGF-β1	1144:1149	arg1	serum					1201:1205	the blood serum	1191:1205	the blood serum	1191:1205	MIA-OA caused a significant increase of TLR-2 soluble form and IL-6, IL-8, TGF-β1, COMP, ACAN, CHI3L1, and CTSK levels in the blood serum; the level of IGF-1, on contrary, decreased.
33459997	4	26	theme	IL-6	828:831	arg1	levels					818:823	The levels	814:823	The levels of IL-6, IL-8, TGF-β1, IGF-1, COMP, ACAN, CHI3L1, CTSK, and TLR-2	814:889	The levels of IL-6, IL-8, TGF-β1, IGF-1, COMP, ACAN, CHI3L1, CTSK, and TLR-2 were measured in serum by enzyme-linked immunosorbent assay.
33459997	5	27	theme	Comp	1035:1038	arg1	expression					1040:1049	Comp expression	1035:1049	Comp expression in the cartilage	1035:1066	Experimental OA caused an upregulation in Tlr2, Tlr4, Nfkb1, and downregulation of Comp expression in the cartilage.
33459997	6	28	from	COMP	1152:1155	arg1	serum					1201:1205	the blood serum	1191:1205	the blood serum	1191:1205	MIA-OA caused a significant increase of TLR-2 soluble form and IL-6, IL-8, TGF-β1, COMP, ACAN, CHI3L1, and CTSK levels in the blood serum; the level of IGF-1, on contrary, decreased.
33459997	10	29	theme	osteoarthritis	1723:1736	arg1	attenuation					1738:1748	osteoarthritis attenuation	1723:1748	osteoarthritis attenuation	1723:1748	The outcomes of our research prove that multistrain live probiotic composition amplifies the positive action of CS in osteoarthritis attenuation and necessitates further investigation with large-scale randomized controlled trial.
33459997	6	30	from	IL-6	1132:1135	arg1	serum					1201:1205	the blood serum	1191:1205	the blood serum	1191:1205	MIA-OA caused a significant increase of TLR-2 soluble form and IL-6, IL-8, TGF-β1, COMP, ACAN, CHI3L1, and CTSK levels in the blood serum; the level of IGF-1, on contrary, decreased.
33459997	2	31	dep	ACAN	614:617	arg1	TLR-2					638:642	TLR-2	638:642	TLR-2	638:642	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	2	31	dep	ACAN	614:617	arg1	CHI3L1					620:625	CHI3L1	620:625	CHI3L1	620:625	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	2	31	dep	ACAN	614:617	arg1	CTSK					628:631	CTSK	628:631	CTSK	628:631	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	2	32	from	OA	690:691	arg1	rats					696:699	rats	696:699	rats	696:699	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	0	33	theme	Experimental	127:138	arg1	Osteoarthritis					140:153	Experimental Osteoarthritis	127:153	Experimental Osteoarthritis	127:153	Probiotic Composition and Chondroitin Sulfate Regulate TLR-2/4-Mediated NF-κB Inflammatory Pathway and Cartilage Metabolism in Experimental Osteoarthritis.
33459997	6	34	from	IL-8	1138:1141	arg1	serum					1201:1205	the blood serum	1191:1205	the blood serum	1191:1205	MIA-OA caused a significant increase of TLR-2 soluble form and IL-6, IL-8, TGF-β1, COMP, ACAN, CHI3L1, and CTSK levels in the blood serum; the level of IGF-1, on contrary, decreased.
33459997	9	35	theme	PB	1499:1500	arg1	use					1492:1494	The combined use	1479:1494	The combined use of PB and CS	1479:1507	The combined use of PB and CS was more effective than separate application approaching above-mentioned parameters to control.
33459997	9	35	theme	PB	1499:1500	arg1	effective					1518:1526	effective	1518:1526	effective	1518:1526	The combined use of PB and CS was more effective than separate application approaching above-mentioned parameters to control.
33459997	3	36	theme	Comp	739:742	arg1	Expression					702:711	Expression	702:711	Expression of Tlr2, Tlr4, Nfkb1, and Comp in cartilage	702:755	Expression of Tlr2, Tlr4, Nfkb1, and Comp in cartilage was analyzed using one-step SYBR Green real-time RT-PCR.
33459997	2	37	theme	sulfate	435:441	arg1	effect					380:385	the effect	376:385	the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats	376:699	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	0	38	theme	Probiotic	0:8	arg1	Composition					10:20	Probiotic Composition	0:20	Probiotic Composition	0:20	Probiotic Composition and Chondroitin Sulfate Regulate TLR-2/4-Mediated NF-κB Inflammatory Pathway and Cartilage Metabolism in Experimental Osteoarthritis.
33459997	1	39	theme	animal	289:294	arg1	studies					306:312	animal and human studies	289:312	animal and human studies	289:312	The therapeutic potential of using probiotics to treat osteoarthritis (OA) has only recently been recognized, with a small number of animal and human studies having been undertaken.
33459997	3	40	theme	one-step	776:783	arg1	RT-PCR					806:811	one-step SYBR Green real-time RT-PCR	776:811	one-step SYBR Green real-time RT-PCR	776:811	Expression of Tlr2, Tlr4, Nfkb1, and Comp in cartilage was analyzed using one-step SYBR Green real-time RT-PCR.
33459997	4	41	theme	COMP	855:858	arg1	levels					818:823	The levels	814:823	The levels of IL-6, IL-8, TGF-β1, IGF-1, COMP, ACAN, CHI3L1, CTSK, and TLR-2	814:889	The levels of IL-6, IL-8, TGF-β1, IGF-1, COMP, ACAN, CHI3L1, CTSK, and TLR-2 were measured in serum by enzyme-linked immunosorbent assay.
33459997	2	42	from	expression	528:537	arg1	cartilage					542:550	cartilage	542:550	cartilage	542:550	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	2	42	from	expression	528:537	arg1	serum					647:651	serum	647:651	serum during monoiodoacetate (MIA)-induced OA in rats	647:699	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	1	43	theme	human	300:304	arg1	studies					306:312	animal and human studies	289:312	animal and human studies	289:312	The therapeutic potential of using probiotics to treat osteoarthritis (OA) has only recently been recognized, with a small number of animal and human studies having been undertaken.
33459997	7	44	theme	Nfkb1	1343:1347	arg1	expressions					1349:1359	reduced Tlr2, Tlr4, and Nfkb1 expressions	1319:1359	reduced Tlr2, Tlr4, and Nfkb1 expressions	1319:1359	Separate administration of PB and CS raised expression of Comp and reduced Tlr2, Tlr4, and Nfkb1 expressions in cartilage.
33459997	4	45	theme	IL-8	834:837	arg1	levels					818:823	The levels	814:823	The levels of IL-6, IL-8, TGF-β1, IGF-1, COMP, ACAN, CHI3L1, CTSK, and TLR-2	814:889	The levels of IL-6, IL-8, TGF-β1, IGF-1, COMP, ACAN, CHI3L1, CTSK, and TLR-2 were measured in serum by enzyme-linked immunosorbent assay.
33459997	2	46	theme	composition	402:412	arg1	effect					380:385	the effect	376:385	the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats	376:699	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	2	47	from	effect	380:385	arg1	Tlr2					495:498	Tlr2	495:498	Tlr2	495:498	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	2	47	from	effect	380:385	arg1	levels					556:561	levels	556:561	levels	556:561	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	2	47	from	effect	380:385	arg1	ACAN					614:617	ACAN	614:617	ACAN	614:617	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	2	47	from	effect	380:385	arg1	Tlr4					501:504	Tlr4	501:504	Tlr4	501:504	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	2	47	from	effect	380:385	arg1	expression					528:537	Comp gene expression	518:537	Comp gene expression	518:537	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	2	47	from	effect	380:385	arg1	Nfkb1					507:511	Nfkb1	507:511	Nfkb1	507:511	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	4	48	theme	TGF-β1	840:845	arg1	levels					818:823	The levels	814:823	The levels of IL-6, IL-8, TGF-β1, IGF-1, COMP, ACAN, CHI3L1, CTSK, and TLR-2	814:889	The levels of IL-6, IL-8, TGF-β1, IGF-1, COMP, ACAN, CHI3L1, CTSK, and TLR-2 were measured in serum by enzyme-linked immunosorbent assay.
33459997	2	49	from	levels	556:561	arg1	cartilage					542:550	cartilage	542:550	cartilage	542:550	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	2	49	from	levels	556:561	arg1	serum					647:651	serum	647:651	serum during monoiodoacetate (MIA)-induced OA in rats	647:699	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	4	50	link	enzyme-linked	917:929	arg1	assay					945:949	enzyme-linked immunosorbent assay	917:949	enzyme-linked immunosorbent assay	917:949	The levels of IL-6, IL-8, TGF-β1, IGF-1, COMP, ACAN, CHI3L1, CTSK, and TLR-2 were measured in serum by enzyme-linked immunosorbent assay.
33459997	0	51	theme	TLR-2/4-Mediated	55:70	arg1	Pathway					91:97	TLR-2/4-Mediated NF-κB Inflammatory Pathway	55:97	TLR-2/4-Mediated NF-κB Inflammatory Pathway	55:97	Probiotic Composition and Chondroitin Sulfate Regulate TLR-2/4-Mediated NF-κB Inflammatory Pathway and Cartilage Metabolism in Experimental Osteoarthritis.
33459997	6	52	from	increase	1097:1104	arg1	serum					1201:1205	the blood serum	1191:1205	the blood serum	1191:1205	MIA-OA caused a significant increase of TLR-2 soluble form and IL-6, IL-8, TGF-β1, COMP, ACAN, CHI3L1, and CTSK levels in the blood serum; the level of IGF-1, on contrary, decreased.
33459997	6	53	from	level	1212:1216	arg1	contrary					1231:1238	contrary	1231:1238	contrary	1231:1238	MIA-OA caused a significant increase of TLR-2 soluble form and IL-6, IL-8, TGF-β1, COMP, ACAN, CHI3L1, and CTSK levels in the blood serum; the level of IGF-1, on contrary, decreased.
33459997	3	54	theme	Green	790:794	arg1	RT-PCR					806:811	one-step SYBR Green real-time RT-PCR	776:811	one-step SYBR Green real-time RT-PCR	776:811	Expression of Tlr2, Tlr4, Nfkb1, and Comp in cartilage was analyzed using one-step SYBR Green real-time RT-PCR.
33459997	4	55	theme	CTSK	875:878	arg1	levels					818:823	The levels	814:823	The levels of IL-6, IL-8, TGF-β1, IGF-1, COMP, ACAN, CHI3L1, CTSK, and TLR-2	814:889	The levels of IL-6, IL-8, TGF-β1, IGF-1, COMP, ACAN, CHI3L1, CTSK, and TLR-2 were measured in serum by enzyme-linked immunosorbent assay.
33459997	8	56	from	serum	1436:1440	arg1	markers					1401:1407	the studied markers	1389:1407	the studied markers of cartilage metabolism in serum	1389:1440	The levels of the studied markers of cartilage metabolism in serum were decreased or increased (IGF-1).
33459997	10	57	theme	further	1767:1773	arg1	investigation					1775:1787	further investigation	1767:1787	further investigation	1767:1787	The outcomes of our research prove that multistrain live probiotic composition amplifies the positive action of CS in osteoarthritis attenuation and necessitates further investigation with large-scale randomized controlled trial.
33459997	8	58	from	metabolism	1422:1431	arg1	serum					1436:1440	serum	1436:1440	serum	1436:1440	The levels of the studied markers of cartilage metabolism in serum were decreased or increased (IGF-1).
33459997	9	59	theme	CS	1506:1507	arg1	use					1492:1494	The combined use	1479:1494	The combined use of PB and CS	1479:1507	The combined use of PB and CS was more effective than separate application approaching above-mentioned parameters to control.
33459997	9	59	theme	CS	1506:1507	arg1	effective					1518:1526	effective	1518:1526	effective	1518:1526	The combined use of PB and CS was more effective than separate application approaching above-mentioned parameters to control.
33459997	4	60	theme	ACAN	861:864	arg1	levels					818:823	The levels	814:823	The levels of IL-6, IL-8, TGF-β1, IGF-1, COMP, ACAN, CHI3L1, CTSK, and TLR-2	814:889	The levels of IL-6, IL-8, TGF-β1, IGF-1, COMP, ACAN, CHI3L1, CTSK, and TLR-2 were measured in serum by enzyme-linked immunosorbent assay.
33459997	6	61	theme	blood	1195:1199	arg1	serum					1201:1205	the blood serum	1191:1205	the blood serum	1191:1205	MIA-OA caused a significant increase of TLR-2 soluble form and IL-6, IL-8, TGF-β1, COMP, ACAN, CHI3L1, and CTSK levels in the blood serum; the level of IGF-1, on contrary, decreased.
33459997	4	62	theme	CHI3L1	867:872	arg1	levels					818:823	The levels	814:823	The levels of IL-6, IL-8, TGF-β1, IGF-1, COMP, ACAN, CHI3L1, CTSK, and TLR-2	814:889	The levels of IL-6, IL-8, TGF-β1, IGF-1, COMP, ACAN, CHI3L1, CTSK, and TLR-2 were measured in serum by enzyme-linked immunosorbent assay.
33459997	4	63	theme	enzyme-linked	917:929	arg1	assay					945:949	enzyme-linked immunosorbent assay	917:949	enzyme-linked immunosorbent assay	917:949	The levels of IL-6, IL-8, TGF-β1, IGF-1, COMP, ACAN, CHI3L1, CTSK, and TLR-2 were measured in serum by enzyme-linked immunosorbent assay.
33459997	5	64	from	upregulation	978:989	arg1	Tlr4					1000:1003	Tlr4	1000:1003	Tlr4	1000:1003	Experimental OA caused an upregulation in Tlr2, Tlr4, Nfkb1, and downregulation of Comp expression in the cartilage.
33459997	5	64	from	upregulation	978:989	arg1	cartilage					1058:1066	the cartilage	1054:1066	the cartilage	1054:1066	Experimental OA caused an upregulation in Tlr2, Tlr4, Nfkb1, and downregulation of Comp expression in the cartilage.
33459997	5	64	from	upregulation	978:989	arg1	Tlr2					994:997	Tlr2	994:997	Tlr2	994:997	Experimental OA caused an upregulation in Tlr2, Tlr4, Nfkb1, and downregulation of Comp expression in the cartilage.
33459997	5	64	from	upregulation	978:989	arg1	Nfkb1					1006:1010	Nfkb1	1006:1010	Nfkb1	1006:1010	Experimental OA caused an upregulation in Tlr2, Tlr4, Nfkb1, and downregulation of Comp expression in the cartilage.
33459997	2	65	theme	cytokines	566:574	arg1	expression					528:537	Comp gene expression	518:537	Comp gene expression	518:537	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	2	65	theme	cytokines	566:574	arg1	Nfkb1					507:511	Nfkb1	507:511	Nfkb1	507:511	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	2	65	theme	cytokines	566:574	arg1	Tlr4					501:504	Tlr4	501:504	Tlr4	501:504	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	2	65	theme	cytokines	566:574	arg1	levels					556:561	levels	556:561	levels	556:561	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	2	65	theme	cytokines	566:574	arg1	Tlr2					495:498	Tlr2	495:498	Tlr2	495:498	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	4	66	theme	immunosorbent	931:943	arg1	assay					945:949	enzyme-linked immunosorbent assay	917:949	enzyme-linked immunosorbent assay	917:949	The levels of IL-6, IL-8, TGF-β1, IGF-1, COMP, ACAN, CHI3L1, CTSK, and TLR-2 were measured in serum by enzyme-linked immunosorbent assay.
33459997	5	67	theme	Experimental	952:963	arg1	OA					965:966	Experimental OA	952:966	Experimental OA	952:966	Experimental OA caused an upregulation in Tlr2, Tlr4, Nfkb1, and downregulation of Comp expression in the cartilage.
33459997	8	68	theme	markers	1401:1407	arg1	levels					1379:1384	The levels	1375:1384	The levels of the studied markers of cartilage metabolism in serum	1375:1440	The levels of the studied markers of cartilage metabolism in serum were decreased or increased (IGF-1).
33459997	10	69	theme	multistrain	1645:1655	arg1	composition					1672:1682	multistrain live probiotic composition	1645:1682	multistrain live probiotic composition	1645:1682	The outcomes of our research prove that multistrain live probiotic composition amplifies the positive action of CS in osteoarthritis attenuation and necessitates further investigation with large-scale randomized controlled trial.
33459997	6	70	theme	form	1123:1126	arg1	CHI3L1					1164:1169	CHI3L1	1164:1169	CHI3L1	1164:1169	MIA-OA caused a significant increase of TLR-2 soluble form and IL-6, IL-8, TGF-β1, COMP, ACAN, CHI3L1, and CTSK levels in the blood serum; the level of IGF-1, on contrary, decreased.
33459997	6	70	theme	form	1123:1126	arg1	TGF-β1					1144:1149	TGF-β1	1144:1149	TGF-β1	1144:1149	MIA-OA caused a significant increase of TLR-2 soluble form and IL-6, IL-8, TGF-β1, COMP, ACAN, CHI3L1, and CTSK levels in the blood serum; the level of IGF-1, on contrary, decreased.
33459997	6	70	theme	form	1123:1126	arg1	COMP					1152:1155	COMP	1152:1155	COMP	1152:1155	MIA-OA caused a significant increase of TLR-2 soluble form and IL-6, IL-8, TGF-β1, COMP, ACAN, CHI3L1, and CTSK levels in the blood serum; the level of IGF-1, on contrary, decreased.
33459997	6	70	theme	form	1123:1126	arg1	increase					1097:1104	a significant increase	1083:1104	a significant increase of TLR-2 soluble form	1083:1126	MIA-OA caused a significant increase of TLR-2 soluble form and IL-6, IL-8, TGF-β1, COMP, ACAN, CHI3L1, and CTSK levels in the blood serum; the level of IGF-1, on contrary, decreased.
33459997	6	70	theme	form	1123:1126	arg1	IL-6					1132:1135	IL-6	1132:1135	IL-6	1132:1135	MIA-OA caused a significant increase of TLR-2 soluble form and IL-6, IL-8, TGF-β1, COMP, ACAN, CHI3L1, and CTSK levels in the blood serum; the level of IGF-1, on contrary, decreased.
33459997	6	70	theme	form	1123:1126	arg1	levels					1181:1186	CTSK levels	1176:1186	CTSK levels	1176:1186	MIA-OA caused a significant increase of TLR-2 soluble form and IL-6, IL-8, TGF-β1, COMP, ACAN, CHI3L1, and CTSK levels in the blood serum; the level of IGF-1, on contrary, decreased.
33459997	6	70	theme	form	1123:1126	arg1	IL-8					1138:1141	IL-8	1138:1141	IL-8	1138:1141	MIA-OA caused a significant increase of TLR-2 soluble form and IL-6, IL-8, TGF-β1, COMP, ACAN, CHI3L1, and CTSK levels in the blood serum; the level of IGF-1, on contrary, decreased.
33459997	8	71	theme	cartilage	1412:1420	arg1	metabolism					1422:1431	cartilage metabolism	1412:1431	cartilage metabolism in serum	1412:1440	The levels of the studied markers of cartilage metabolism in serum were decreased or increased (IGF-1).
33459997	2	72	dep	cytokines	566:574	arg1	IL-8					583:586	IL-8	583:586	IL-8	583:586	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	2	72	dep	cytokines	566:574	arg1	IL-6					577:580	IL-6	577:580	IL-6	577:580	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	2	72	dep	cytokines	566:574	arg1	IGF-1					597:601	IGF-1	597:601	IGF-1	597:601	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	2	72	dep	cytokines	566:574	arg1	TGF-β1					589:594	TGF-β1	589:594	TGF-β1	589:594	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	5	73	from	cartilage	1058:1066	arg1	upregulation					978:989	an upregulation	975:989	an upregulation in Tlr2, Tlr4, Nfkb1,	975:1011	Experimental OA caused an upregulation in Tlr2, Tlr4, Nfkb1, and downregulation of Comp expression in the cartilage.
33459997	5	73	from	cartilage	1058:1066	arg1	downregulation					1017:1030	downregulation	1017:1030	downregulation of Comp expression in the cartilage	1017:1066	Experimental OA caused an upregulation in Tlr2, Tlr4, Nfkb1, and downregulation of Comp expression in the cartilage.
33459997	6	74	theme	TLR-2	1109:1113	arg1	form					1123:1126	TLR-2 soluble form	1109:1126	TLR-2 soluble form	1109:1126	MIA-OA caused a significant increase of TLR-2 soluble form and IL-6, IL-8, TGF-β1, COMP, ACAN, CHI3L1, and CTSK levels in the blood serum; the level of IGF-1, on contrary, decreased.
33459997	6	75	theme	IGF-1	1221:1225	arg1	level					1212:1216	the level	1208:1216	the level	1208:1216	MIA-OA caused a significant increase of TLR-2 soluble form and IL-6, IL-8, TGF-β1, COMP, ACAN, CHI3L1, and CTSK levels in the blood serum; the level of IGF-1, on contrary, decreased.
33459997	7	76	theme	reduced	1319:1325	arg1	expressions					1349:1359	reduced Tlr2, Tlr4, and Nfkb1 expressions	1319:1359	reduced Tlr2, Tlr4, and Nfkb1 expressions	1319:1359	Separate administration of PB and CS raised expression of Comp and reduced Tlr2, Tlr4, and Nfkb1 expressions in cartilage.
33459997	0	77	from	Pathway	91:97	arg1	Osteoarthritis					140:153	Experimental Osteoarthritis	127:153	Experimental Osteoarthritis	127:153	Probiotic Composition and Chondroitin Sulfate Regulate TLR-2/4-Mediated NF-κB Inflammatory Pathway and Cartilage Metabolism in Experimental Osteoarthritis.
33459997	7	78	theme	Comp	1310:1313	arg1	expression					1296:1305	expression	1296:1305	expression of Comp and reduced Tlr2, Tlr4, and Nfkb1 expressions in cartilage	1296:1372	Separate administration of PB and CS raised expression of Comp and reduced Tlr2, Tlr4, and Nfkb1 expressions in cartilage.
33459997	2	79	theme	Comp	518:521	arg1	expression					528:537	Comp gene expression	518:537	Comp gene expression	518:537	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	10	80	theme	probiotic	1662:1670	arg1	composition					1672:1682	multistrain live probiotic composition	1645:1682	multistrain live probiotic composition	1645:1682	The outcomes of our research prove that multistrain live probiotic composition amplifies the positive action of CS in osteoarthritis attenuation and necessitates further investigation with large-scale randomized controlled trial.
33459997	1	81	theme	therapeutic	160:170	arg1	potential					172:180	The therapeutic potential	156:180	The therapeutic potential of using probiotics to treat osteoarthritis (OA)	156:229	The therapeutic potential of using probiotics to treat osteoarthritis (OA) has only recently been recognized, with a small number of animal and human studies having been undertaken.
33459997	3	82	theme	Tlr4	722:725	arg1	Expression					702:711	Expression	702:711	Expression of Tlr2, Tlr4, Nfkb1, and Comp in cartilage	702:755	Expression of Tlr2, Tlr4, Nfkb1, and Comp in cartilage was analyzed using one-step SYBR Green real-time RT-PCR.
33459997	2	83	from	Tlr2	495:498	arg1	cartilage					542:550	cartilage	542:550	cartilage	542:550	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	2	83	from	Tlr2	495:498	arg1	serum					647:651	serum	647:651	serum during monoiodoacetate (MIA)-induced OA in rats	647:699	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	3	84	theme	Tlr2	716:719	arg1	Expression					702:711	Expression	702:711	Expression of Tlr2, Tlr4, Nfkb1, and Comp in cartilage	702:755	Expression of Tlr2, Tlr4, Nfkb1, and Comp in cartilage was analyzed using one-step SYBR Green real-time RT-PCR.
33459997	7	85	theme	CS	1286:1287	arg1	administration					1261:1274	Separate administration	1252:1274	Separate administration of PB and CS	1252:1287	Separate administration of PB and CS raised expression of Comp and reduced Tlr2, Tlr4, and Nfkb1 expressions in cartilage.
33459997	10	86	theme	positive	1698:1705	arg1	action					1707:1712	the positive action	1694:1712	the positive action of CS	1694:1718	The outcomes of our research prove that multistrain live probiotic composition amplifies the positive action of CS in osteoarthritis attenuation and necessitates further investigation with large-scale randomized controlled trial.
33459997	0	87	theme	Cartilage	103:111	arg1	Metabolism					113:122	Cartilage Metabolism	103:122	Cartilage Metabolism	103:122	Probiotic Composition and Chondroitin Sulfate Regulate TLR-2/4-Mediated NF-κB Inflammatory Pathway and Cartilage Metabolism in Experimental Osteoarthritis.
33459997	7	88	theme	PB	1279:1280	arg1	administration					1261:1274	Separate administration	1252:1274	Separate administration of PB and CS	1252:1287	Separate administration of PB and CS raised expression of Comp and reduced Tlr2, Tlr4, and Nfkb1 expressions in cartilage.
33459997	5	89	theme	expression	1040:1049	arg1	upregulation					978:989	an upregulation	975:989	an upregulation in Tlr2, Tlr4, Nfkb1,	975:1011	Experimental OA caused an upregulation in Tlr2, Tlr4, Nfkb1, and downregulation of Comp expression in the cartilage.
33459997	5	89	theme	expression	1040:1049	arg1	downregulation					1017:1030	downregulation	1017:1030	downregulation of Comp expression in the cartilage	1017:1066	Experimental OA caused an upregulation in Tlr2, Tlr4, Nfkb1, and downregulation of Comp expression in the cartilage.
33459997	3	90	theme	Nfkb1	728:732	arg1	Expression					702:711	Expression	702:711	Expression of Tlr2, Tlr4, Nfkb1, and Comp in cartilage	702:755	Expression of Tlr2, Tlr4, Nfkb1, and Comp in cartilage was analyzed using one-step SYBR Green real-time RT-PCR.
33459997	2	91	theme	chondroitin	423:433	arg1	sulfate					435:441	chondroitin sulfate	423:441	chondroitin sulfate	423:441	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	0	92	theme	Chondroitin	26:36	arg1	Sulfate					38:44	Chondroitin Sulfate	26:44	Chondroitin Sulfate	26:44	Probiotic Composition and Chondroitin Sulfate Regulate TLR-2/4-Mediated NF-κB Inflammatory Pathway and Cartilage Metabolism in Experimental Osteoarthritis.
33459997	3	93	theme	SYBR	785:788	arg1	RT-PCR					806:811	one-step SYBR Green real-time RT-PCR	776:811	one-step SYBR Green real-time RT-PCR	776:811	Expression of Tlr2, Tlr4, Nfkb1, and Comp in cartilage was analyzed using one-step SYBR Green real-time RT-PCR.
33459997	1	94	theme	studies	306:312	arg1	number					279:284	a small number	271:284	a small number of animal and human studies	271:312	The therapeutic potential of using probiotics to treat osteoarthritis (OA) has only recently been recognized, with a small number of animal and human studies having been undertaken.
33459997	7	95	theme	Separate	1252:1259	arg1	administration					1261:1274	Separate administration	1252:1274	Separate administration of PB and CS	1252:1287	Separate administration of PB and CS raised expression of Comp and reduced Tlr2, Tlr4, and Nfkb1 expressions in cartilage.
33459997	7	96	theme	expressions	1349:1359	arg1	expression					1296:1305	expression	1296:1305	expression of Comp and reduced Tlr2, Tlr4, and Nfkb1 expressions in cartilage	1296:1372	Separate administration of PB and CS raised expression of Comp and reduced Tlr2, Tlr4, and Nfkb1 expressions in cartilage.
33459997	3	97	from	Expression	702:711	arg1	cartilage					747:755	cartilage	747:755	cartilage	747:755	Expression of Tlr2, Tlr4, Nfkb1, and Comp in cartilage was analyzed using one-step SYBR Green real-time RT-PCR.
33459997	8	98	from	markers	1401:1407	arg1	serum					1436:1440	serum	1436:1440	serum	1436:1440	The levels of the studied markers of cartilage metabolism in serum were decreased or increased (IGF-1).
33459997	2	99	theme	probiotic	392:400	arg1	CS					444:445	CS	444:445	CS	444:445	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	2	99	theme	probiotic	392:400	arg1	composition					402:412	a probiotic composition	390:412	a probiotic composition (PB)	390:417	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	2	99	theme	probiotic	392:400	arg1	PB					415:416	PB	415:416	PB	415:416	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	0	100	theme	NF-κB	72:76	arg1	Pathway					91:97	TLR-2/4-Mediated NF-κB Inflammatory Pathway	55:97	TLR-2/4-Mediated NF-κB Inflammatory Pathway	55:97	Probiotic Composition and Chondroitin Sulfate Regulate TLR-2/4-Mediated NF-κB Inflammatory Pathway and Cartilage Metabolism in Experimental Osteoarthritis.
33459997	7	101	from	expression	1296:1305	arg1	cartilage					1364:1372	cartilage	1364:1372	cartilage	1364:1372	Separate administration of PB and CS raised expression of Comp and reduced Tlr2, Tlr4, and Nfkb1 expressions in cartilage.
33459997	3	102	theme	real-time	796:804	arg1	RT-PCR					806:811	one-step SYBR Green real-time RT-PCR	776:811	one-step SYBR Green real-time RT-PCR	776:811	Expression of Tlr2, Tlr4, Nfkb1, and Comp in cartilage was analyzed using one-step SYBR Green real-time RT-PCR.
33459997	2	103	theme	-induced	681:688	arg1	OA					690:691	monoiodoacetate (MIA)-induced OA	660:691	monoiodoacetate (MIA)-induced OA in rats	660:699	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	2	104	from	Tlr4	501:504	arg1	cartilage					542:550	cartilage	542:550	cartilage	542:550	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	2	104	from	Tlr4	501:504	arg1	serum					647:651	serum	647:651	serum during monoiodoacetate (MIA)-induced OA in rats	647:699	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	8	105	dep	increased	1460:1468	arg1	IGF-1					1471:1475	IGF-1	1471:1475	IGF-1	1471:1475	The levels of the studied markers of cartilage metabolism in serum were decreased or increased (IGF-1).
33459997	9	106	theme	separate	1533:1540	arg1	application					1542:1552	separate application	1533:1552	separate application approaching above-mentioned parameters to control	1533:1602	The combined use of PB and CS was more effective than separate application approaching above-mentioned parameters to control.
33459997	2	107	from	Nfkb1	507:511	arg1	cartilage					542:550	cartilage	542:550	cartilage	542:550	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	2	107	from	Nfkb1	507:511	arg1	serum					647:651	serum	647:651	serum during monoiodoacetate (MIA)-induced OA in rats	647:699	The aim of this study was to describe the effect of a probiotic composition (PB) and chondroitin sulfate (CS), administered separately or in combination, on Tlr2, Tlr4, Nfkb1, and Comp gene expression in cartilage and levels of cytokines (IL-6, IL-8, TGF-β1, IGF-1) and COMP, ACAN, CHI3L1, CTSK, and TLR-2 in serum during monoiodoacetate (MIA)-induced OA in rats.
33459997	4	108	theme	IGF-1	848:852	arg1	levels					818:823	The levels	814:823	The levels of IL-6, IL-8, TGF-β1, IGF-1, COMP, ACAN, CHI3L1, CTSK, and TLR-2	814:889	The levels of IL-6, IL-8, TGF-β1, IGF-1, COMP, ACAN, CHI3L1, CTSK, and TLR-2 were measured in serum by enzyme-linked immunosorbent assay.
33925595	11	0	theme	industrial	2006:2015	arg1	scale					2017:2021	a pilot or industrial scale	1995:2021	a pilot or industrial scale	1995:2021	Such optimization is of great significance for the selection of key nutrients and their concentrations in order to increase the pigment production at a pilot or industrial scale.
33925595	6	1	theme	1.39	1123:1126	arg1	pigments					1093:1100	red pigments	1089:1100	red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent)	1089:1195	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	6	1	theme	1.39	1123:1126	arg1	equivalent					1143:1152	1.39 g/L quinizarin equivalent	1123:1152	1.39 g/L quinizarin equivalent	1123:1152	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	0	2	from	Fermentation	188:199	arg1	Optimization					77:88	Optimization	77:88	Optimization of Pigment Production in the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation	77:199	OVAT Analysis and Response Surface Methodology Based on Nutrient Sources for Optimization of Pigment Production in the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation.
33925595	11	3	theme	pilot	1997:2001	arg1	scale					2017:2021	a pilot or industrial scale	1995:2021	a pilot or industrial scale	1995:2021	Such optimization is of great significance for the selection of key nutrients and their concentrations in order to increase the pigment production at a pilot or industrial scale.
33925595	11	4	theme	Such	1845:1848	arg1	optimization					1850:1861	Such optimization	1845:1861	Such optimization	1845:1861	Such optimization is of great significance for the selection of key nutrients and their concentrations in order to increase the pigment production at a pilot or industrial scale.
33925595	11	5	theme	nutrients	1913:1921	arg1	selection					1896:1904	the selection	1892:1904	the selection of key nutrients and their concentrations in order to increase the pigment production at a pilot or industrial scale	1892:2021	Such optimization is of great significance for the selection of key nutrients and their concentrations in order to increase the pigment production at a pilot or industrial scale.
33925595	1	6	theme	species	295:301	arg1	productions					384:394	controlled massive productions	365:394	controlled massive productions	365:394	Pigment production from filamentous fungi is gaining interest due to the diversity of fungal species, the variety of compounds synthesized, and the possibility of controlled massive productions.
33925595	1	6	theme	species	295:301	arg1	variety					308:314	the variety	304:314	the variety of compounds synthesized	304:339	Pigment production from filamentous fungi is gaining interest due to the diversity of fungal species, the variety of compounds synthesized, and the possibility of controlled massive productions.
33925595	1	6	theme	species	295:301	arg1	possibility					350:360	the possibility	346:360	the possibility of controlled massive productions	346:394	Pigment production from filamentous fungi is gaining interest due to the diversity of fungal species, the variety of compounds synthesized, and the possibility of controlled massive productions.
33925595	1	6	theme	species	295:301	arg1	diversity					275:283	the diversity	271:283	the diversity of fungal species	271:301	Pigment production from filamentous fungi is gaining interest due to the diversity of fungal species, the variety of compounds synthesized, and the possibility of controlled massive productions.
33925595	1	6	theme	species	295:301	arg1	compounds					319:327	compounds	319:327	compounds synthesized	319:339	Pigment production from filamentous fungi is gaining interest due to the diversity of fungal species, the variety of compounds synthesized, and the possibility of controlled massive productions.
33925595	1	6	theme	species	295:301	arg1	species					295:301	fungal species	288:301	fungal species	288:301	Pigment production from filamentous fungi is gaining interest due to the diversity of fungal species, the variety of compounds synthesized, and the possibility of controlled massive productions.
33925595	4	7	theme	several	884:890	arg1	sources					902:908	several nutrients sources	884:908	several nutrients sources	884:908	The present work is the first in an attempt to optimize pigments production in a culture of the marine-derived T. albobiverticillius 30548, under the influence of several nutrients sources.
33925595	10	8	theme	g/L	1829:1831	arg1	MgSO4·7H2O					1833:1842	0.2 g/L MgSO4·7H2O	1825:1842	0.2 g/L MgSO4·7H2O	1825:1842	The predicted maximum response for OPY (1.43 g/L), RPY (2.59 g/L), and DBW (15.98 g/L) were obtained at 3 g/L yeast extract, 1 g/L K2HPO4, and 0.2 g/L MgSO4·7H2O.
33925595	3	9	theme	pigment	583:589	arg1	production					591:600	Optimizing pigment production	572:600	Optimizing pigment production	572:600	Optimizing pigment production from fungal strains grown on different carbon and nitrogen sources, using statistical methods, is widespread nowadays.
33925595	1	10	theme	Pigment	202:208	arg1	production					210:219	Pigment production	202:219	Pigment production from filamentous fungi	202:242	Pigment production from filamentous fungi is gaining interest due to the diversity of fungal species, the variety of compounds synthesized, and the possibility of controlled massive productions.
33925595	4	11	theme	sources	902:908	arg1	influence					871:879	the influence	867:879	the influence of several nutrients sources	867:908	The present work is the first in an attempt to optimize pigments production in a culture of the marine-derived T. albobiverticillius 30548, under the influence of several nutrients sources.
33925595	6	12	theme	equivalent	1185:1194	arg1	pigment					1177:1183	2.44 g/L Red Yeast pigment equivalent	1158:1194	2.44 g/L Red Yeast pigment equivalent	1158:1194	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	6	12	theme	equivalent	1185:1194	arg1	pigments					1093:1100	red pigments	1089:1100	red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent)	1089:1195	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	4	13	theme	present	725:731	arg1	work					733:736	The present work	721:736	The present work	721:736	The present work is the first in an attempt to optimize pigments production in a culture of the marine-derived T. albobiverticillius 30548, under the influence of several nutrients sources.
33925595	4	13	theme	present	725:731	arg1	first					745:749	first	745:749	first	745:749	The present work is the first in an attempt to optimize pigments production in a culture of the marine-derived T. albobiverticillius 30548, under the influence of several nutrients sources.
33925595	6	14	theme	Yeast	1171:1175	arg1	pigment					1177:1183	2.44 g/L Red Yeast pigment equivalent	1158:1194	2.44 g/L Red Yeast pigment equivalent	1158:1194	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	6	14	theme	Yeast	1171:1175	arg1	pigments					1093:1100	red pigments	1089:1100	red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent)	1089:1195	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	6	15	theme	yeast	1021:1025	arg1	extract					1027:1033	yeast extract	1021:1033	yeast extract	1021:1033	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	8	16	theme	central	1429:1435	arg1	design					1447:1452	A five-level central composite design	1416:1452	A five-level central composite design (CCD)	1416:1458	A five-level central composite design (CCD) and a response surface methodology (RSM) were applied to evaluate the optimal concentrations and interactive effects between selected nutrients.
33925595	8	16	theme	central	1429:1435	arg1	CCD					1455:1457	CCD	1455:1457	CCD	1455:1457	A five-level central composite design (CCD) and a response surface methodology (RSM) were applied to evaluate the optimal concentrations and interactive effects between selected nutrients.
33925595	0	17	theme	Fungus	134:139	arg1	Fermentation					188:199	the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation	115:199	the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation	115:199	OVAT Analysis and Response Surface Methodology Based on Nutrient Sources for Optimization of Pigment Production in the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation.
33925595	3	18	theme	nitrogen	652:659	arg1	sources					661:667	different carbon and nitrogen sources	631:667	sources	661:667	Optimizing pigment production from fungal strains grown on different carbon and nitrogen sources, using statistical methods, is widespread nowadays.
33925595	0	19	theme	albobiverticillius	153:170	arg1	Fermentation					188:199	the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation	115:199	the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation	115:199	OVAT Analysis and Response Surface Methodology Based on Nutrient Sources for Optimization of Pigment Production in the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation.
33925595	5	20	theme	OVAT	983:986	arg1	analysis					989:996	the one-variable-at-a-time (OVAT) analysis	955:996	the one-variable-at-a-time (OVAT) analysis	955:996	Nutrient combinations were screened through the one-variable-at-a-time (OVAT) analysis.
33925595	11	21	theme	pigment	1973:1979	arg1	production					1981:1990	the pigment production	1969:1990	the pigment production	1969:1990	Such optimization is of great significance for the selection of key nutrients and their concentrations in order to increase the pigment production at a pilot or industrial scale.
33925595	0	22	from	Production	101:110	arg1	Fermentation					188:199	the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation	115:199	the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation	115:199	OVAT Analysis and Response Surface Methodology Based on Nutrient Sources for Optimization of Pigment Production in the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation.
33925595	3	23	from	strains	614:620	arg1	production					591:600	Optimizing pigment production	572:600	Optimizing pigment production	572:600	Optimizing pigment production from fungal strains grown on different carbon and nitrogen sources, using statistical methods, is widespread nowadays.
33925595	0	24	theme	Submerged	178:186	arg1	Fermentation					188:199	the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation	115:199	the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation	115:199	OVAT Analysis and Response Surface Methodology Based on Nutrient Sources for Optimization of Pigment Production in the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation.
33925595	3	25	theme	statistical	676:686	arg1	methods					688:694	statistical methods	676:694	statistical methods	676:694	Optimizing pigment production from fungal strains grown on different carbon and nitrogen sources, using statistical methods, is widespread nowadays.
33925595	6	26	theme	higher	1209:1214	arg1	DBW					1229:1231	DBW	1229:1231	DBW	1229:1231	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	6	26	theme	higher	1209:1214	arg1	biomass					1220:1226	higher dry biomass	1209:1226	higher dry biomass (DBW) (6.60 g/L)	1209:1243	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	6	26	theme	higher	1209:1214	arg1	g/L					1240:1242	6.60 g/L	1235:1242	6.60 g/L	1235:1242	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	0	27	theme	Nutrient	56:63	arg1	Sources					65:71	Nutrient Sources	56:71	Nutrient Sources for Optimization of Pigment Production in the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation	56:199	OVAT Analysis and Response Surface Methodology Based on Nutrient Sources for Optimization of Pigment Production in the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation.
33925595	6	28	theme	pigments	1070:1077	arg1	DBW					1229:1231	DBW	1229:1231	DBW	1229:1231	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	6	28	theme	pigments	1070:1077	arg1	biomass					1220:1226	higher dry biomass	1209:1226	higher dry biomass (DBW) (6.60 g/L)	1209:1243	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	6	28	theme	pigments	1070:1077	arg1	yield					1054:1058	a maximum yield	1044:1058	a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent)	1044:1195	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	6	28	theme	pigments	1070:1077	arg1	g/L					1240:1242	6.60 g/L	1235:1242	6.60 g/L	1235:1242	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	8	29	theme	response	1466:1473	arg1	methodology					1483:1493	a response surface methodology	1464:1493	a response surface methodology (RSM)	1464:1499	A five-level central composite design (CCD) and a response surface methodology (RSM) were applied to evaluate the optimal concentrations and interactive effects between selected nutrients.
33925595	8	29	theme	response	1466:1473	arg1	RSM					1496:1498	RSM	1496:1498	RSM	1496:1498	A five-level central composite design (CCD) and a response surface methodology (RSM) were applied to evaluate the optimal concentrations and interactive effects between selected nutrients.
33925595	5	30	theme	one-variable-at-a-time	959:980	arg1	analysis					989:996	the one-variable-at-a-time (OVAT) analysis	955:996	the one-variable-at-a-time (OVAT) analysis	955:996	Nutrient combinations were screened through the one-variable-at-a-time (OVAT) analysis.
33925595	6	31	theme	dry	1216:1218	arg1	DBW					1229:1231	DBW	1229:1231	DBW	1229:1231	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	6	31	theme	dry	1216:1218	arg1	biomass					1220:1226	higher dry biomass	1209:1226	higher dry biomass (DBW) (6.60 g/L)	1209:1243	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	6	31	theme	dry	1216:1218	arg1	g/L					1240:1242	6.60 g/L	1235:1242	6.60 g/L	1235:1242	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	1	32	theme	massive	376:382	arg1	productions					384:394	controlled massive productions	365:394	controlled massive productions	365:394	Pigment production from filamentous fungi is gaining interest due to the diversity of fungal species, the variety of compounds synthesized, and the possibility of controlled massive productions.
33925595	6	33	theme	maximum	1046:1052	arg1	yield					1054:1058	a maximum yield	1044:1058	a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent)	1044:1195	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	2	34	theme	industrial	547:556	arg1	applications					558:569	industrial applications	547:569	industrial applications	547:569	The Talaromyces species produce a large panel of metabolites, including Monascus-like azaphilone pigments, with potential use as natural colorants in industrial applications.
33925595	0	35	theme	Production	101:110	arg1	Optimization					77:88	Optimization	77:88	Optimization of Pigment Production in the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation	77:199	OVAT Analysis and Response Surface Methodology Based on Nutrient Sources for Optimization of Pigment Production in the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation.
33925595	2	36	theme	azaphilone	483:492	arg1	pigments					494:501	Monascus-like azaphilone pigments	469:501	Monascus-like azaphilone pigments	469:501	The Talaromyces species produce a large panel of metabolites, including Monascus-like azaphilone pigments, with potential use as natural colorants in industrial applications.
33925595	4	37	theme	pigments	777:784	arg1	production					786:795	pigments production	777:795	pigments production in a culture of the marine-derived T. albobiverticillius 30548	777:858	The present work is the first in an attempt to optimize pigments production in a culture of the marine-derived T. albobiverticillius 30548, under the influence of several nutrients sources.
33925595	0	38	theme	OVAT	0:3	arg1	Analysis					5:12	OVAT Analysis	0:12	OVAT Analysis	0:12	OVAT Analysis and Response Surface Methodology Based on Nutrient Sources for Optimization of Pigment Production in the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation.
33925595	9	39	theme	chosen	1656:1661	arg1	model					1675:1679	the chosen statistical model	1652:1679	the chosen statistical model	1652:1679	The experimental results were well fitted with the chosen statistical model.
33925595	10	40	theme	3	1786:1786	arg1	g/L					1788:1790	g/L	1788:1790	g/L	1788:1790	The predicted maximum response for OPY (1.43 g/L), RPY (2.59 g/L), and DBW (15.98 g/L) were obtained at 3 g/L yeast extract, 1 g/L K2HPO4, and 0.2 g/L MgSO4·7H2O.
33925595	2	41	theme	metabolites	446:456	arg1	panel					437:441	a large panel	429:441	a large panel of metabolites, including Monascus-like azaphilone pigments	429:501	The Talaromyces species produce a large panel of metabolites, including Monascus-like azaphilone pigments, with potential use as natural colorants in industrial applications.
33925595	6	42	theme	pigments	1093:1100	arg1	DBW					1229:1231	DBW	1229:1231	DBW	1229:1231	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	6	42	theme	pigments	1093:1100	arg1	biomass					1220:1226	higher dry biomass	1209:1226	higher dry biomass (DBW) (6.60 g/L)	1209:1243	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	6	42	theme	pigments	1093:1100	arg1	yield					1054:1058	a maximum yield	1044:1058	a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent)	1044:1195	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	6	42	theme	pigments	1093:1100	arg1	g/L					1240:1242	6.60 g/L	1235:1242	6.60 g/L	1235:1242	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	2	43	theme	potential	509:517	arg1	use					519:521	potential use	509:521	potential use as natural colorants in industrial applications	509:569	The Talaromyces species produce a large panel of metabolites, including Monascus-like azaphilone pigments, with potential use as natural colorants in industrial applications.
33925595	6	44	theme	red	1089:1091	arg1	pigments					1093:1100	red pigments	1089:1100	red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent)	1089:1195	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	6	44	theme	red	1089:1091	arg1	pigment					1177:1183	2.44 g/L Red Yeast pigment equivalent	1158:1194	2.44 g/L Red Yeast pigment equivalent	1158:1194	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	6	44	theme	red	1089:1091	arg1	equivalent					1143:1152	1.39 g/L quinizarin equivalent	1123:1152	1.39 g/L quinizarin equivalent	1123:1152	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	6	44	theme	red	1089:1091	arg1	RPY					1103:1105	RPY	1103:1105	RPY	1103:1105	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	0	45	theme	Surface	27:33	arg1	Methodology					35:45	Response Surface Methodology	18:45	Response Surface Methodology	18:45	OVAT Analysis and Response Surface Methodology Based on Nutrient Sources for Optimization of Pigment Production in the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation.
33925595	1	46	theme	compounds	319:327	arg1	productions					384:394	controlled massive productions	365:394	controlled massive productions	365:394	Pigment production from filamentous fungi is gaining interest due to the diversity of fungal species, the variety of compounds synthesized, and the possibility of controlled massive productions.
33925595	1	46	theme	compounds	319:327	arg1	variety					308:314	the variety	304:314	the variety of compounds synthesized	304:339	Pigment production from filamentous fungi is gaining interest due to the diversity of fungal species, the variety of compounds synthesized, and the possibility of controlled massive productions.
33925595	1	46	theme	compounds	319:327	arg1	possibility					350:360	the possibility	346:360	the possibility of controlled massive productions	346:394	Pigment production from filamentous fungi is gaining interest due to the diversity of fungal species, the variety of compounds synthesized, and the possibility of controlled massive productions.
33925595	1	46	theme	compounds	319:327	arg1	diversity					275:283	the diversity	271:283	the diversity of fungal species	271:301	Pigment production from filamentous fungi is gaining interest due to the diversity of fungal species, the variety of compounds synthesized, and the possibility of controlled massive productions.
33925595	1	46	theme	compounds	319:327	arg1	compounds					319:327	compounds	319:327	compounds synthesized	319:339	Pigment production from filamentous fungi is gaining interest due to the diversity of fungal species, the variety of compounds synthesized, and the possibility of controlled massive productions.
33925595	1	46	theme	compounds	319:327	arg1	species					295:301	fungal species	288:301	fungal species	288:301	Pigment production from filamentous fungi is gaining interest due to the diversity of fungal species, the variety of compounds synthesized, and the possibility of controlled massive productions.
33925595	4	47	link	marine-derived	817:830	arg1	30548					854:858	the marine-derived T. albobiverticillius 30548	813:858	the marine-derived T. albobiverticillius 30548	813:858	The present work is the first in an attempt to optimize pigments production in a culture of the marine-derived T. albobiverticillius 30548, under the influence of several nutrients sources.
33925595	6	48	theme	2.44	1158:1161	arg1	g/L					1163:1165	g/L	1163:1165	g/L	1163:1165	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	8	49	theme	interactive	1557:1567	arg1	effects					1569:1575	interactive effects	1557:1575	interactive effects	1557:1575	A five-level central composite design (CCD) and a response surface methodology (RSM) were applied to evaluate the optimal concentrations and interactive effects between selected nutrients.
33925595	4	50	theme	T.	832:833	arg1	30548					854:858	the marine-derived T. albobiverticillius 30548	813:858	the marine-derived T. albobiverticillius 30548	813:858	The present work is the first in an attempt to optimize pigments production in a culture of the marine-derived T. albobiverticillius 30548, under the influence of several nutrients sources.
33925595	10	51	theme	predicted	1686:1694	arg1	response					1704:1711	The predicted maximum response	1682:1711	The predicted maximum response for OPY (1.43 g/L), RPY (2.59 g/L), and DBW (15.98 g/L)	1682:1767	The predicted maximum response for OPY (1.43 g/L), RPY (2.59 g/L), and DBW (15.98 g/L) were obtained at 3 g/L yeast extract, 1 g/L K2HPO4, and 0.2 g/L MgSO4·7H2O.
33925595	4	52	from	attempt	757:763	arg1	work					733:736	The present work	721:736	The present work	721:736	The present work is the first in an attempt to optimize pigments production in a culture of the marine-derived T. albobiverticillius 30548, under the influence of several nutrients sources.
33925595	4	52	from	attempt	757:763	arg1	first					745:749	first	745:749	first	745:749	The present work is the first in an attempt to optimize pigments production in a culture of the marine-derived T. albobiverticillius 30548, under the influence of several nutrients sources.
33925595	7	53	dep	extract	1283:1289	arg1	MgSO4·7H2O					1303:1312	MgSO4·7H2O	1303:1312	MgSO4·7H2O	1303:1312	Significant medium components (yeast extract, K2HPO4 and MgSO4·7H2O) were also identified from one-variable-at-a-time (OVAT) analysis for pigment and biomass production.
33925595	7	53	dep	extract	1283:1289	arg1	K2HPO4					1292:1297	K2HPO4	1292:1297	K2HPO4	1292:1297	Significant medium components (yeast extract, K2HPO4 and MgSO4·7H2O) were also identified from one-variable-at-a-time (OVAT) analysis for pigment and biomass production.
33925595	7	54	theme	medium	1258:1263	arg1	components					1265:1274	Significant medium components	1246:1274	Significant medium components (yeast extract, K2HPO4 and MgSO4·7H2O)	1246:1313	Significant medium components (yeast extract, K2HPO4 and MgSO4·7H2O) were also identified from one-variable-at-a-time (OVAT) analysis for pigment and biomass production.
33925595	10	55	theme	yeast	1792:1796	arg1	extract					1798:1804	3 g/L yeast extract	1786:1804	3 g/L yeast extract	1786:1804	The predicted maximum response for OPY (1.43 g/L), RPY (2.59 g/L), and DBW (15.98 g/L) were obtained at 3 g/L yeast extract, 1 g/L K2HPO4, and 0.2 g/L MgSO4·7H2O.
33925595	4	56	theme	30548	854:858	arg1	culture					802:808	a culture	800:808	a culture of the marine-derived T. albobiverticillius 30548	800:858	The present work is the first in an attempt to optimize pigments production in a culture of the marine-derived T. albobiverticillius 30548, under the influence of several nutrients sources.
33925595	5	57	theme	Nutrient	911:918	arg1	combinations					920:931	Nutrient combinations	911:931	Nutrient combinations	911:931	Nutrient combinations were screened through the one-variable-at-a-time (OVAT) analysis.
33925595	6	58	theme	g/L	1128:1130	arg1	pigments					1093:1100	red pigments	1089:1100	red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent)	1089:1195	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	6	58	theme	g/L	1128:1130	arg1	equivalent					1143:1152	1.39 g/L quinizarin equivalent	1123:1152	1.39 g/L quinizarin equivalent	1123:1152	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	10	59	theme	g/L	1809:1811	arg1	K2HPO4					1813:1818	1 g/L K2HPO4	1807:1818	1 g/L K2HPO4	1807:1818	The predicted maximum response for OPY (1.43 g/L), RPY (2.59 g/L), and DBW (15.98 g/L) were obtained at 3 g/L yeast extract, 1 g/L K2HPO4, and 0.2 g/L MgSO4·7H2O.
33925595	11	60	theme	key	1909:1911	arg1	nutrients					1913:1921	key nutrients	1909:1921	key nutrients	1909:1921	Such optimization is of great significance for the selection of key nutrients and their concentrations in order to increase the pigment production at a pilot or industrial scale.
33925595	1	61	theme	fungal	288:293	arg1	species					295:301	fungal species	288:301	fungal species	288:301	Pigment production from filamentous fungi is gaining interest due to the diversity of fungal species, the variety of compounds synthesized, and the possibility of controlled massive productions.
33925595	3	62	theme	Optimizing	572:581	arg1	production					591:600	Optimizing pigment production	572:600	Optimizing pigment production	572:600	Optimizing pigment production from fungal strains grown on different carbon and nitrogen sources, using statistical methods, is widespread nowadays.
33925595	9	63	theme	statistical	1663:1673	arg1	model					1675:1679	the chosen statistical model	1652:1679	the chosen statistical model	1652:1679	The experimental results were well fitted with the chosen statistical model.
33925595	10	64	theme	0.2	1825:1827	arg1	g/L					1829:1831	g/L	1829:1831	g/L	1829:1831	The predicted maximum response for OPY (1.43 g/L), RPY (2.59 g/L), and DBW (15.98 g/L) were obtained at 3 g/L yeast extract, 1 g/L K2HPO4, and 0.2 g/L MgSO4·7H2O.
33925595	11	65	theme	concentrations	1933:1946	arg1	selection					1896:1904	the selection	1892:1904	the selection of key nutrients and their concentrations in order to increase the pigment production at a pilot or industrial scale	1892:2021	Such optimization is of great significance for the selection of key nutrients and their concentrations in order to increase the pigment production at a pilot or industrial scale.
33925595	4	66	theme	nutrients	892:900	arg1	sources					902:908	several nutrients sources	884:908	several nutrients sources	884:908	The present work is the first in an attempt to optimize pigments production in a culture of the marine-derived T. albobiverticillius 30548, under the influence of several nutrients sources.
33925595	7	67	theme	biomass	1396:1402	arg1	production					1404:1413	biomass production	1396:1413	biomass production	1396:1413	Significant medium components (yeast extract, K2HPO4 and MgSO4·7H2O) were also identified from one-variable-at-a-time (OVAT) analysis for pigment and biomass production.
33925595	0	68	theme	Marine-Derived	119:132	arg1	Fermentation					188:199	the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation	115:199	the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation	115:199	OVAT Analysis and Response Surface Methodology Based on Nutrient Sources for Optimization of Pigment Production in the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation.
33925595	3	69	theme	fungal	607:612	arg1	strains					614:620	fungal strains	607:620	fungal strains grown on different carbon and nitrogen sources	607:667	Optimizing pigment production from fungal strains grown on different carbon and nitrogen sources, using statistical methods, is widespread nowadays.
33925595	1	70	theme	filamentous	226:236	arg1	fungi					238:242	filamentous fungi	226:242	filamentous fungi	226:242	Pigment production from filamentous fungi is gaining interest due to the diversity of fungal species, the variety of compounds synthesized, and the possibility of controlled massive productions.
33925595	8	71	theme	composite	1437:1445	arg1	design					1447:1452	A five-level central composite design	1416:1452	A five-level central composite design (CCD)	1416:1458	A five-level central composite design (CCD) and a response surface methodology (RSM) were applied to evaluate the optimal concentrations and interactive effects between selected nutrients.
33925595	8	71	theme	composite	1437:1445	arg1	CCD					1455:1457	CCD	1455:1457	CCD	1455:1457	A five-level central composite design (CCD) and a response surface methodology (RSM) were applied to evaluate the optimal concentrations and interactive effects between selected nutrients.
33925595	6	72	theme	Red	1167:1169	arg1	pigment					1177:1183	2.44 g/L Red Yeast pigment equivalent	1158:1194	2.44 g/L Red Yeast pigment equivalent	1158:1194	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	6	72	theme	Red	1167:1169	arg1	pigments					1093:1100	red pigments	1089:1100	red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent)	1089:1195	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	0	73	theme	Talaromyces	141:151	arg1	Fermentation					188:199	the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation	115:199	the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation	115:199	OVAT Analysis and Response Surface Methodology Based on Nutrient Sources for Optimization of Pigment Production in the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation.
33925595	2	74	from	colorants	534:542	arg1	applications					558:569	industrial applications	547:569	industrial applications	547:569	The Talaromyces species produce a large panel of metabolites, including Monascus-like azaphilone pigments, with potential use as natural colorants in industrial applications.
33925595	8	75	dep	concentrations	1538:1551	arg1	the					1526:1528	the	1526:1528	the	1526:1528	A five-level central composite design (CCD) and a response surface methodology (RSM) were applied to evaluate the optimal concentrations and interactive effects between selected nutrients.
33925595	3	76	theme	different	631:639	arg1	carbon					641:646	different carbon and nitrogen sources	631:667	carbon	641:646	Optimizing pigment production from fungal strains grown on different carbon and nitrogen sources, using statistical methods, is widespread nowadays.
33925595	7	77	theme	OVAT	1365:1368	arg1	analysis					1371:1378	one-variable-at-a-time (OVAT) analysis	1341:1378	one-variable-at-a-time (OVAT) analysis for pigment and biomass production	1341:1413	Significant medium components (yeast extract, K2HPO4 and MgSO4·7H2O) were also identified from one-variable-at-a-time (OVAT) analysis for pigment and biomass production.
33925595	1	78	from	fungi	238:242	arg1	production					210:219	Pigment production	202:219	Pigment production from filamentous fungi	202:242	Pigment production from filamentous fungi is gaining interest due to the diversity of fungal species, the variety of compounds synthesized, and the possibility of controlled massive productions.
33925595	3	79	dep	production	591:600	arg1	using					670:674	using	670:674	using statistical methods	670:694	Optimizing pigment production from fungal strains grown on different carbon and nitrogen sources, using statistical methods, is widespread nowadays.
33925595	7	80	theme	one-variable-at-a-time	1341:1362	arg1	analysis					1371:1378	one-variable-at-a-time (OVAT) analysis	1341:1378	one-variable-at-a-time (OVAT) analysis for pigment and biomass production	1341:1413	Significant medium components (yeast extract, K2HPO4 and MgSO4·7H2O) were also identified from one-variable-at-a-time (OVAT) analysis for pigment and biomass production.
33925595	11	81	theme	great	1869:1873	arg1	significance					1875:1886	great significance	1869:1886	great significance	1869:1886	Such optimization is of great significance for the selection of key nutrients and their concentrations in order to increase the pigment production at a pilot or industrial scale.
33925595	2	82	theme	natural	526:532	arg1	colorants					534:542	natural colorants	526:542	natural colorants in industrial applications	526:569	The Talaromyces species produce a large panel of metabolites, including Monascus-like azaphilone pigments, with potential use as natural colorants in industrial applications.
33925595	8	83	theme	surface	1475:1481	arg1	methodology					1483:1493	a response surface methodology	1464:1493	a response surface methodology (RSM)	1464:1499	A five-level central composite design (CCD) and a response surface methodology (RSM) were applied to evaluate the optimal concentrations and interactive effects between selected nutrients.
33925595	8	83	theme	surface	1475:1481	arg1	RSM					1496:1498	RSM	1496:1498	RSM	1496:1498	A five-level central composite design (CCD) and a response surface methodology (RSM) were applied to evaluate the optimal concentrations and interactive effects between selected nutrients.
33925595	6	84	theme	orange	1063:1068	arg1	OPY					1080:1082	OPY	1080:1082	OPY	1080:1082	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	6	84	theme	orange	1063:1068	arg1	pigments					1070:1077	orange pigments	1063:1077	orange pigments (OPY)	1063:1083	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	0	85	from	Optimization	77:88	arg1	Fermentation					188:199	the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation	115:199	the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation	115:199	OVAT Analysis and Response Surface Methodology Based on Nutrient Sources for Optimization of Pigment Production in the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation.
33925595	0	86	theme	Pigment	93:99	arg1	Production					101:110	Pigment Production	93:110	Pigment Production in the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation	93:199	OVAT Analysis and Response Surface Methodology Based on Nutrient Sources for Optimization of Pigment Production in the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation.
33925595	1	87	theme	controlled	365:374	arg1	productions					384:394	controlled massive productions	365:394	controlled massive productions	365:394	Pigment production from filamentous fungi is gaining interest due to the diversity of fungal species, the variety of compounds synthesized, and the possibility of controlled massive productions.
33925595	1	88	theme	productions	384:394	arg1	productions					384:394	controlled massive productions	365:394	controlled massive productions	365:394	Pigment production from filamentous fungi is gaining interest due to the diversity of fungal species, the variety of compounds synthesized, and the possibility of controlled massive productions.
33925595	1	88	theme	productions	384:394	arg1	variety					308:314	the variety	304:314	the variety of compounds synthesized	304:339	Pigment production from filamentous fungi is gaining interest due to the diversity of fungal species, the variety of compounds synthesized, and the possibility of controlled massive productions.
33925595	1	88	theme	productions	384:394	arg1	possibility					350:360	the possibility	346:360	the possibility of controlled massive productions	346:394	Pigment production from filamentous fungi is gaining interest due to the diversity of fungal species, the variety of compounds synthesized, and the possibility of controlled massive productions.
33925595	1	88	theme	productions	384:394	arg1	diversity					275:283	the diversity	271:283	the diversity of fungal species	271:301	Pigment production from filamentous fungi is gaining interest due to the diversity of fungal species, the variety of compounds synthesized, and the possibility of controlled massive productions.
33925595	1	88	theme	productions	384:394	arg1	compounds					319:327	compounds	319:327	compounds synthesized	319:339	Pigment production from filamentous fungi is gaining interest due to the diversity of fungal species, the variety of compounds synthesized, and the possibility of controlled massive productions.
33925595	1	88	theme	productions	384:394	arg1	species					295:301	fungal species	288:301	fungal species	288:301	Pigment production from filamentous fungi is gaining interest due to the diversity of fungal species, the variety of compounds synthesized, and the possibility of controlled massive productions.
33925595	7	89	dep	components	1265:1274	arg1	extract					1283:1289	yeast extract	1277:1289	yeast extract	1277:1289	Significant medium components (yeast extract, K2HPO4 and MgSO4·7H2O) were also identified from one-variable-at-a-time (OVAT) analysis for pigment and biomass production.
33925595	6	90	dep	pigments	1093:1100	arg1	pigment					1177:1183	2.44 g/L Red Yeast pigment equivalent	1158:1194	2.44 g/L Red Yeast pigment equivalent	1158:1194	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	6	90	dep	pigments	1093:1100	arg1	pigments					1093:1100	red pigments	1089:1100	red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent)	1089:1195	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	6	90	dep	pigments	1093:1100	arg1	equivalent					1143:1152	1.39 g/L quinizarin equivalent	1123:1152	1.39 g/L quinizarin equivalent	1123:1152	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	4	91	from	production	786:795	arg1	culture					802:808	a culture	800:808	a culture of the marine-derived T. albobiverticillius 30548	800:858	The present work is the first in an attempt to optimize pigments production in a culture of the marine-derived T. albobiverticillius 30548, under the influence of several nutrients sources.
33925595	2	92	theme	Monascus-like	469:481	arg1	pigments					494:501	Monascus-like azaphilone pigments	469:501	Monascus-like azaphilone pigments	469:501	The Talaromyces species produce a large panel of metabolites, including Monascus-like azaphilone pigments, with potential use as natural colorants in industrial applications.
33925595	9	93	theme	experimental	1609:1620	arg1	results					1622:1628	The experimental results	1605:1628	The experimental results	1605:1628	The experimental results were well fitted with the chosen statistical model.
33925595	2	94	theme	Talaromyces	401:411	arg1	species					413:419	The Talaromyces species	397:419	The Talaromyces species	397:419	The Talaromyces species produce a large panel of metabolites, including Monascus-like azaphilone pigments, with potential use as natural colorants in industrial applications.
33925595	0	95	theme	Response	18:25	arg1	Methodology					35:45	Response Surface Methodology	18:45	Response Surface Methodology	18:45	OVAT Analysis and Response Surface Methodology Based on Nutrient Sources for Optimization of Pigment Production in the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation.
33925595	8	96	theme	five-level	1418:1427	arg1	design					1447:1452	A five-level central composite design	1416:1452	A five-level central composite design (CCD)	1416:1458	A five-level central composite design (CCD) and a response surface methodology (RSM) were applied to evaluate the optimal concentrations and interactive effects between selected nutrients.
33925595	8	96	theme	five-level	1418:1427	arg1	CCD					1455:1457	CCD	1455:1457	CCD	1455:1457	A five-level central composite design (CCD) and a response surface methodology (RSM) were applied to evaluate the optimal concentrations and interactive effects between selected nutrients.
33925595	8	97	theme	optimal	1530:1536	arg1	concentrations					1538:1551	optimal concentrations	1530:1551	optimal concentrations	1530:1551	A five-level central composite design (CCD) and a response surface methodology (RSM) were applied to evaluate the optimal concentrations and interactive effects between selected nutrients.
33925595	10	98	theme	g/L	1788:1790	arg1	extract					1798:1804	3 g/L yeast extract	1786:1804	3 g/L yeast extract	1786:1804	The predicted maximum response for OPY (1.43 g/L), RPY (2.59 g/L), and DBW (15.98 g/L) were obtained at 3 g/L yeast extract, 1 g/L K2HPO4, and 0.2 g/L MgSO4·7H2O.
33925595	7	99	theme	yeast	1277:1281	arg1	extract					1283:1289	yeast extract	1277:1289	yeast extract	1277:1289	Significant medium components (yeast extract, K2HPO4 and MgSO4·7H2O) were also identified from one-variable-at-a-time (OVAT) analysis for pigment and biomass production.
33925595	2	100	theme	large	431:435	arg1	panel					437:441	a large panel	429:441	a large panel of metabolites, including Monascus-like azaphilone pigments	429:501	The Talaromyces species produce a large panel of metabolites, including Monascus-like azaphilone pigments, with potential use as natural colorants in industrial applications.
33925595	6	101	theme	g/L	1163:1165	arg1	pigment					1177:1183	2.44 g/L Red Yeast pigment equivalent	1158:1194	2.44 g/L Red Yeast pigment equivalent	1158:1194	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	6	101	theme	g/L	1163:1165	arg1	pigments					1093:1100	red pigments	1089:1100	red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent)	1089:1195	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	10	102	theme	1	1807:1807	arg1	g/L					1809:1811	g/L	1809:1811	g/L	1809:1811	The predicted maximum response for OPY (1.43 g/L), RPY (2.59 g/L), and DBW (15.98 g/L) were obtained at 3 g/L yeast extract, 1 g/L K2HPO4, and 0.2 g/L MgSO4·7H2O.
33925595	10	103	theme	maximum	1696:1702	arg1	response					1704:1711	The predicted maximum response	1682:1711	The predicted maximum response for OPY (1.43 g/L), RPY (2.59 g/L), and DBW (15.98 g/L)	1682:1767	The predicted maximum response for OPY (1.43 g/L), RPY (2.59 g/L), and DBW (15.98 g/L) were obtained at 3 g/L yeast extract, 1 g/L K2HPO4, and 0.2 g/L MgSO4·7H2O.
33925595	7	104	theme	Significant	1246:1256	arg1	components					1265:1274	Significant medium components	1246:1274	Significant medium components (yeast extract, K2HPO4 and MgSO4·7H2O)	1246:1313	Significant medium components (yeast extract, K2HPO4 and MgSO4·7H2O) were also identified from one-variable-at-a-time (OVAT) analysis for pigment and biomass production.
33925595	8	105	theme	selected	1585:1592	arg1	nutrients					1594:1602	selected nutrients	1585:1602	selected nutrients	1585:1602	A five-level central composite design (CCD) and a response surface methodology (RSM) were applied to evaluate the optimal concentrations and interactive effects between selected nutrients.
33925595	4	106	theme	marine-derived	817:830	arg1	30548					854:858	the marine-derived T. albobiverticillius 30548	813:858	the marine-derived T. albobiverticillius 30548	813:858	The present work is the first in an attempt to optimize pigments production in a culture of the marine-derived T. albobiverticillius 30548, under the influence of several nutrients sources.
33925595	6	107	theme	quinizarin	1132:1141	arg1	pigments					1093:1100	red pigments	1089:1100	red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent)	1089:1195	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	6	107	theme	quinizarin	1132:1141	arg1	equivalent					1143:1152	1.39 g/L quinizarin equivalent	1123:1152	1.39 g/L quinizarin equivalent	1123:1152	Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L).
33925595	4	108	theme	albobiverticillius	835:852	arg1	30548					854:858	the marine-derived T. albobiverticillius 30548	813:858	the marine-derived T. albobiverticillius 30548	813:858	The present work is the first in an attempt to optimize pigments production in a culture of the marine-derived T. albobiverticillius 30548, under the influence of several nutrients sources.
33925595	4	109	from	first	745:749	arg1	attempt					757:763	an attempt to optimize pigments production in a culture of the marine-derived T. albobiverticillius 30548	754:858	an attempt to optimize pigments production in a culture of the marine-derived T. albobiverticillius 30548	754:858	The present work is the first in an attempt to optimize pigments production in a culture of the marine-derived T. albobiverticillius 30548, under the influence of several nutrients sources.
34765476	9	0	theme	main	1621:1624	arg1	reason					1626:1631	the main reason	1617:1631	the main reason for the decreased ability of glycan degradation in the PKU group	1617:1696	The remarkable capability of genus Bacteroides to use complex recalcitrant glycans may be the main reason for the decreased ability of glycan degradation in the PKU group.
34765476	9	0	theme	main	1621:1624	arg1	capability					1542:1551	The remarkable capability	1527:1551	The remarkable capability of genus Bacteroides to use complex recalcitrant glycans	1527:1608	The remarkable capability of genus Bacteroides to use complex recalcitrant glycans may be the main reason for the decreased ability of glycan degradation in the PKU group.
34765476	0	1	from	abundance	6:14	arg1	microbiota					44:53	gut microbiota	40:53	gut microbiota	40:53	A low abundance of genus Bacteroides in gut microbiota is negatively correlated with blood phenylalanine levels in Uygur patients with phenylketonuria.
34765476	2	2	theme	metabolic	354:362	arg1	pathways					364:371	metabolic pathways	354:371	metabolic pathways	354:371	Our study examined gut microbiota characteristics and metabolic pathways, and their correlations with clinical phenotypes in a high-incidence population.
34765476	1	3	theme	microbial	238:246	arg1	communities					248:258	gut microbial communities	234:258	gut microbial communities	234:258	BACKGROUND A low-phenylalanine (Phe) diet affects the metabolism and diversity of gut microbial communities in children with phenylketonuria (PKU).
34765476	7	4	theme	PKU	1345:1347	arg1	group					1349:1353	the PKU group	1341:1353	the PKU group	1341:1353	The microbial function prediction of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways exhibited a decreased ability of glycan degradation and glutamate metabolism in the PKU group.
34765476	3	5	theme	ribosomal	520:528	arg1	rRNA					535:538	rRNA	535:538	rRNA	535:538	METHODS We assessed clinical phenotypes and gut microbiota by 16S ribosomal RNA (rRNA) sequencing, and performed a correlation analysis between phenotype and gut microbiota in a PKU group (n=11) and a healthy group (n=11).
34765476	3	5	theme	ribosomal	520:528	arg1	RNA					530:532	ribosomal RNA	520:532	ribosomal RNA (rRNA) sequencing	520:550	METHODS We assessed clinical phenotypes and gut microbiota by 16S ribosomal RNA (rRNA) sequencing, and performed a correlation analysis between phenotype and gut microbiota in a PKU group (n=11) and a healthy group (n=11).
34765476	1	6	theme	communities	248:258	arg1	diversity					221:229	diversity	221:229	diversity	221:229	BACKGROUND A low-phenylalanine (Phe) diet affects the metabolism and diversity of gut microbial communities in children with phenylketonuria (PKU).
34765476	1	6	theme	communities	248:258	arg1	metabolism					206:215	metabolism	206:215	metabolism	206:215	BACKGROUND A low-phenylalanine (Phe) diet affects the metabolism and diversity of gut microbial communities in children with phenylketonuria (PKU).
34765476	0	7	theme	blood	85:89	arg1	levels					105:110	blood phenylalanine levels	85:110	blood phenylalanine levels in Uygur patients with phenylketonuria	85:149	A low abundance of genus Bacteroides in gut microbiota is negatively correlated with blood phenylalanine levels in Uygur patients with phenylketonuria.
34765476	2	8	with	correlations	384:395	arg1	phenotypes					411:420	clinical phenotypes	402:420	clinical phenotypes	402:420	Our study examined gut microbiota characteristics and metabolic pathways, and their correlations with clinical phenotypes in a high-incidence population.
34765476	9	9	theme	degradation	1669:1679	arg1	ability					1651:1657	the decreased ability	1637:1657	the decreased ability of glycan degradation in the PKU group	1637:1696	The remarkable capability of genus Bacteroides to use complex recalcitrant glycans may be the main reason for the decreased ability of glycan degradation in the PKU group.
34765476	7	10	theme	degradation	1301:1311	arg1	ability					1283:1289	a decreased ability	1271:1289	a decreased ability of glycan degradation and glutamate metabolism	1271:1336	The microbial function prediction of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways exhibited a decreased ability of glycan degradation and glutamate metabolism in the PKU group.
34765476	7	11	theme	decreased	1273:1281	arg1	ability					1283:1289	a decreased ability	1271:1289	a decreased ability of glycan degradation and glutamate metabolism	1271:1336	The microbial function prediction of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways exhibited a decreased ability of glycan degradation and glutamate metabolism in the PKU group.
34765476	1	12	from	metabolism	206:215	arg1	children					263:270	children	263:270	children with phenylketonuria (PKU)	263:297	BACKGROUND A low-phenylalanine (Phe) diet affects the metabolism and diversity of gut microbial communities in children with phenylketonuria (PKU).
34765476	4	13	theme	microbiota	723:732	arg1	diversity					734:742	significantly lower microbiota diversity	703:742	significantly lower microbiota diversity	703:742	RESULTS The PKU group had significantly lower microbiota diversity than the healthy group (Pshannon=0.014).
34765476	4	14	dep	RESULTS	677:683	arg1	had					699:701	had	699:701	had significantly lower microbiota diversity than the healthy group (Pshannon=0.014)	699:782	RESULTS The PKU group had significantly lower microbiota diversity than the healthy group (Pshannon=0.014).
34765476	9	15	theme	decreased	1641:1649	arg1	ability					1651:1657	the decreased ability	1637:1657	the decreased ability of glycan degradation in the PKU group	1637:1696	The remarkable capability of genus Bacteroides to use complex recalcitrant glycans may be the main reason for the decreased ability of glycan degradation in the PKU group.
34765476	8	16	theme	genus	1395:1399	arg1	Bacteroide					1401:1410	genus Bacteroide	1395:1410	genus Bacteroide	1395:1410	CONCLUSIONS Our findings revealed that genus Bacteroide was not only in extremely low abundance in the PKU group, but was also negatively correlated with blood Phe level.
34765476	6	17	theme	Bacteroidetes	1064:1076	arg1	level					1049:1053	a significantly decreased level	1023:1053	a significantly decreased level of genus Bacteroidetes (P<0.0001) in the PKU group	1023:1104	Further, a significantly decreased level of genus Bacteroidetes (P<0.0001) in the PKU group was negatively correlated with blood Phe level (P=0.014).
34765476	5	18	theme	healthy	853:859	arg1	groups					861:866	the PKU and healthy groups	841:866	groups	861:866	Phylum-level composition differed significantly between the PKU and healthy groups (Firmicutes: 44.3% vs. 43.1%; Actinobacteria: 25.9% vs. 3.3%; Bacteroidetes: 16.6% vs. 53.2%; and Proteobacteria: 10.9% vs. 0.12%, respectively).
34765476	1	19	theme	low-phenylalanine	165:181	arg1	diet					189:192	A low-phenylalanine (Phe) diet	163:192	A low-phenylalanine (Phe) diet	163:192	BACKGROUND A low-phenylalanine (Phe) diet affects the metabolism and diversity of gut microbial communities in children with phenylketonuria (PKU).
34765476	0	20	theme	phenylalanine	91:103	arg1	levels					105:110	blood phenylalanine levels	85:110	blood phenylalanine levels in Uygur patients with phenylketonuria	85:149	A low abundance of genus Bacteroides in gut microbiota is negatively correlated with blood phenylalanine levels in Uygur patients with phenylketonuria.
34765476	7	21	theme	glutamate	1317:1325	arg1	metabolism					1327:1336	glutamate metabolism	1317:1336	glutamate metabolism	1317:1336	The microbial function prediction of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways exhibited a decreased ability of glycan degradation and glutamate metabolism in the PKU group.
34765476	6	22	theme	Phe	1143:1145	arg1	P=0.014					1154:1160	P=0.014	1154:1160	P=0.014	1154:1160	Further, a significantly decreased level of genus Bacteroidetes (P<0.0001) in the PKU group was negatively correlated with blood Phe level (P=0.014).
34765476	6	22	theme	Phe	1143:1145	arg1	level					1147:1151	blood Phe level	1137:1151	blood Phe level (P=0.014)	1137:1161	Further, a significantly decreased level of genus Bacteroidetes (P<0.0001) in the PKU group was negatively correlated with blood Phe level (P=0.014).
34765476	6	23	theme	decreased	1039:1047	arg1	level					1049:1053	a significantly decreased level	1023:1053	a significantly decreased level of genus Bacteroidetes (P<0.0001) in the PKU group	1023:1104	Further, a significantly decreased level of genus Bacteroidetes (P<0.0001) in the PKU group was negatively correlated with blood Phe level (P=0.014).
34765476	7	24	theme	glycan	1294:1299	arg1	degradation					1301:1311	glycan degradation	1294:1311	glycan degradation	1294:1311	The microbial function prediction of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways exhibited a decreased ability of glycan degradation and glutamate metabolism in the PKU group.
34765476	4	25	theme	healthy	753:759	arg1	Pshannon=0.014					768:781	Pshannon=0.014	768:781	Pshannon=0.014	768:781	RESULTS The PKU group had significantly lower microbiota diversity than the healthy group (Pshannon=0.014).
34765476	4	25	theme	healthy	753:759	arg1	group					761:765	the healthy group	749:765	the healthy group (Pshannon=0.014)	749:782	RESULTS The PKU group had significantly lower microbiota diversity than the healthy group (Pshannon=0.014).
34765476	0	26	theme	low	2:4	arg1	abundance					6:14	A low abundance	0:14	A low abundance of genus Bacteroides in gut microbiota	0:53	A low abundance of genus Bacteroides in gut microbiota is negatively correlated with blood phenylalanine levels in Uygur patients with phenylketonuria.
34765476	7	27	theme	metabolism	1327:1336	arg1	ability					1283:1289	a decreased ability	1271:1289	a decreased ability of glycan degradation and glutamate metabolism	1271:1336	The microbial function prediction of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways exhibited a decreased ability of glycan degradation and glutamate metabolism in the PKU group.
34765476	6	28	theme	blood	1137:1141	arg1	P=0.014					1154:1160	P=0.014	1154:1160	P=0.014	1154:1160	Further, a significantly decreased level of genus Bacteroidetes (P<0.0001) in the PKU group was negatively correlated with blood Phe level (P=0.014).
34765476	6	28	theme	blood	1137:1141	arg1	level					1147:1151	blood Phe level	1137:1151	blood Phe level (P=0.014)	1137:1161	Further, a significantly decreased level of genus Bacteroidetes (P<0.0001) in the PKU group was negatively correlated with blood Phe level (P=0.014).
34765476	1	29	dep	BACKGROUND	152:161	arg1	affects					194:200	affects	194:200	affects the metabolism and diversity of gut microbial communities in children with phenylketonuria (PKU)	194:297	BACKGROUND A low-phenylalanine (Phe) diet affects the metabolism and diversity of gut microbial communities in children with phenylketonuria (PKU).
34765476	9	30	theme	recalcitrant	1589:1600	arg1	glycans					1602:1608	complex recalcitrant glycans	1581:1608	complex recalcitrant glycans	1581:1608	The remarkable capability of genus Bacteroides to use complex recalcitrant glycans may be the main reason for the decreased ability of glycan degradation in the PKU group.
34765476	1	31	theme	Phe	184:186	arg1	diet					189:192	A low-phenylalanine (Phe) diet	163:192	A low-phenylalanine (Phe) diet	163:192	BACKGROUND A low-phenylalanine (Phe) diet affects the metabolism and diversity of gut microbial communities in children with phenylketonuria (PKU).
34765476	9	32	theme	glycan	1662:1667	arg1	degradation					1669:1679	glycan degradation	1662:1679	glycan degradation	1662:1679	The remarkable capability of genus Bacteroides to use complex recalcitrant glycans may be the main reason for the decreased ability of glycan degradation in the PKU group.
34765476	8	33	dep	CONCLUSIONS	1356:1366	arg1	revealed					1381:1388	revealed	1381:1388	revealed that genus Bacteroide was not only in extremely low abundance in the PKU group, but was also negatively correlated with blood Phe level	1381:1524	CONCLUSIONS Our findings revealed that genus Bacteroide was not only in extremely low abundance in the PKU group, but was also negatively correlated with blood Phe level.
34765476	8	34	from	abundance	1442:1450	arg1	group					1463:1467	the PKU group	1455:1467	the PKU group	1455:1467	CONCLUSIONS Our findings revealed that genus Bacteroide was not only in extremely low abundance in the PKU group, but was also negatively correlated with blood Phe level.
34765476	3	35	theme	correlation	569:579	arg1	analysis					581:588	a correlation analysis	567:588	a correlation analysis between phenotype and gut microbiota	567:625	METHODS We assessed clinical phenotypes and gut microbiota by 16S ribosomal RNA (rRNA) sequencing, and performed a correlation analysis between phenotype and gut microbiota in a PKU group (n=11) and a healthy group (n=11).
34765476	7	36	theme	microbial	1168:1176	arg1	prediction					1187:1196	The microbial function prediction	1164:1196	The microbial function prediction of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways	1164:1259	The microbial function prediction of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways exhibited a decreased ability of glycan degradation and glutamate metabolism in the PKU group.
34765476	5	37	theme	Phylum-level	785:796	arg1	composition					798:808	Phylum-level composition	785:808	Phylum-level composition	785:808	Phylum-level composition differed significantly between the PKU and healthy groups (Firmicutes: 44.3% vs. 43.1%; Actinobacteria: 25.9% vs. 3.3%; Bacteroidetes: 16.6% vs. 53.2%; and Proteobacteria: 10.9% vs. 0.12%, respectively).
34765476	0	38	theme	Uygur	115:119	arg1	patients					121:128	Uygur patients	115:128	Uygur patients with phenylketonuria	115:149	A low abundance of genus Bacteroides in gut microbiota is negatively correlated with blood phenylalanine levels in Uygur patients with phenylketonuria.
34765476	8	39	dep	only	1420:1423	arg1	not					1416:1418	not	1416:1418	not	1416:1418	CONCLUSIONS Our findings revealed that genus Bacteroide was not only in extremely low abundance in the PKU group, but was also negatively correlated with blood Phe level.
34765476	4	40	theme	lower	717:721	arg1	diversity					734:742	significantly lower microbiota diversity	703:742	significantly lower microbiota diversity	703:742	RESULTS The PKU group had significantly lower microbiota diversity than the healthy group (Pshannon=0.014).
34765476	3	41	theme	clinical	474:481	arg1	phenotypes					483:492	clinical phenotypes	474:492	clinical phenotypes	474:492	METHODS We assessed clinical phenotypes and gut microbiota by 16S ribosomal RNA (rRNA) sequencing, and performed a correlation analysis between phenotype and gut microbiota in a PKU group (n=11) and a healthy group (n=11).
34765476	2	42	theme	high-incidence	427:440	arg1	population					442:451	a high-incidence population	425:451	a high-incidence population	425:451	Our study examined gut microbiota characteristics and metabolic pathways, and their correlations with clinical phenotypes in a high-incidence population.
34765476	8	43	theme	low	1438:1440	arg1	abundance					1442:1450	extremely low abundance	1428:1450	extremely low abundance	1428:1450	CONCLUSIONS Our findings revealed that genus Bacteroide was not only in extremely low abundance in the PKU group, but was also negatively correlated with blood Phe level.
34765476	9	44	theme	Bacteroides	1562:1572	arg1	reason					1626:1631	the main reason	1617:1631	the main reason for the decreased ability of glycan degradation in the PKU group	1617:1696	The remarkable capability of genus Bacteroides to use complex recalcitrant glycans may be the main reason for the decreased ability of glycan degradation in the PKU group.
34765476	9	44	theme	Bacteroides	1562:1572	arg1	capability					1542:1551	The remarkable capability	1527:1551	The remarkable capability of genus Bacteroides to use complex recalcitrant glycans	1527:1608	The remarkable capability of genus Bacteroides to use complex recalcitrant glycans may be the main reason for the decreased ability of glycan degradation in the PKU group.
34765476	5	45	dep	Firmicutes	869:878	arg1	%					918:918	25.9%	914:918	25.9%	914:918	Phylum-level composition differed significantly between the PKU and healthy groups (Firmicutes: 44.3% vs. 43.1%; Actinobacteria: 25.9% vs. 3.3%; Bacteroidetes: 16.6% vs. 53.2%; and Proteobacteria: 10.9% vs. 0.12%, respectively).
34765476	5	45	dep	Firmicutes	869:878	arg1	%					895:895	44.3% vs. 43.1%	881:895	%	895:895	Phylum-level composition differed significantly between the PKU and healthy groups (Firmicutes: 44.3% vs. 43.1%; Actinobacteria: 25.9% vs. 3.3%; Bacteroidetes: 16.6% vs. 53.2%; and Proteobacteria: 10.9% vs. 0.12%, respectively).
34765476	5	45	dep	Firmicutes	869:878	arg1	%					996:996	0.12%	992:996	0.12%	992:996	Phylum-level composition differed significantly between the PKU and healthy groups (Firmicutes: 44.3% vs. 43.1%; Actinobacteria: 25.9% vs. 3.3%; Bacteroidetes: 16.6% vs. 53.2%; and Proteobacteria: 10.9% vs. 0.12%, respectively).
34765476	5	45	dep	Firmicutes	869:878	arg1	%					949:949	16.6% vs. 53.2%	945:959	%	949:949	Phylum-level composition differed significantly between the PKU and healthy groups (Firmicutes: 44.3% vs. 43.1%; Actinobacteria: 25.9% vs. 3.3%; Bacteroidetes: 16.6% vs. 53.2%; and Proteobacteria: 10.9% vs. 0.12%, respectively).
34765476	5	45	dep	Firmicutes	869:878	arg1	Actinobacteria					898:911	Actinobacteria	898:911	Actinobacteria	898:911	Phylum-level composition differed significantly between the PKU and healthy groups (Firmicutes: 44.3% vs. 43.1%; Actinobacteria: 25.9% vs. 3.3%; Bacteroidetes: 16.6% vs. 53.2%; and Proteobacteria: 10.9% vs. 0.12%, respectively).
34765476	5	45	dep	Firmicutes	869:878	arg1	%					986:986	Proteobacteria: 10.9%	966:986	Proteobacteria: 10.9%	966:986	Phylum-level composition differed significantly between the PKU and healthy groups (Firmicutes: 44.3% vs. 43.1%; Actinobacteria: 25.9% vs. 3.3%; Bacteroidetes: 16.6% vs. 53.2%; and Proteobacteria: 10.9% vs. 0.12%, respectively).
34765476	5	45	dep	Firmicutes	869:878	arg1	Bacteroidetes					930:942	Bacteroidetes	930:942	Bacteroidetes	930:942	Phylum-level composition differed significantly between the PKU and healthy groups (Firmicutes: 44.3% vs. 43.1%; Actinobacteria: 25.9% vs. 3.3%; Bacteroidetes: 16.6% vs. 53.2%; and Proteobacteria: 10.9% vs. 0.12%, respectively).
34765476	5	45	dep	Firmicutes	869:878	arg1	%					885:885	44.3% vs. 43.1%	881:895	%	885:885	Phylum-level composition differed significantly between the PKU and healthy groups (Firmicutes: 44.3% vs. 43.1%; Actinobacteria: 25.9% vs. 3.3%; Bacteroidetes: 16.6% vs. 53.2%; and Proteobacteria: 10.9% vs. 0.12%, respectively).
34765476	3	46	theme	PKU	632:634	arg1	n=11					643:646	n=11	643:646	n=11	643:646	METHODS We assessed clinical phenotypes and gut microbiota by 16S ribosomal RNA (rRNA) sequencing, and performed a correlation analysis between phenotype and gut microbiota in a PKU group (n=11) and a healthy group (n=11).
34765476	3	46	theme	PKU	632:634	arg1	group					636:640	a PKU group	630:640	a PKU group (n=11)	630:647	METHODS We assessed clinical phenotypes and gut microbiota by 16S ribosomal RNA (rRNA) sequencing, and performed a correlation analysis between phenotype and gut microbiota in a PKU group (n=11) and a healthy group (n=11).
34765476	7	47	dep	Genes	1227:1231	arg1	pathways					1252:1259	pathways	1252:1259	pathways	1252:1259	The microbial function prediction of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways exhibited a decreased ability of glycan degradation and glutamate metabolism in the PKU group.
34765476	0	48	theme	Bacteroides	25:35	arg1	abundance					6:14	A low abundance	0:14	A low abundance of genus Bacteroides in gut microbiota	0:53	A low abundance of genus Bacteroides in gut microbiota is negatively correlated with blood phenylalanine levels in Uygur patients with phenylketonuria.
34765476	4	49	contain	had	699:701	arg1	group					693:697	The PKU group	685:697	The PKU group	685:697	RESULTS The PKU group had significantly lower microbiota diversity than the healthy group (Pshannon=0.014).
34765476	4	49	contain	had	699:701	arg2	diversity					734:742	significantly lower microbiota diversity	703:742	significantly lower microbiota diversity	703:742	RESULTS The PKU group had significantly lower microbiota diversity than the healthy group (Pshannon=0.014).
34765476	9	50	from	ability	1651:1657	arg1	group					1692:1696	the PKU group	1684:1696	the PKU group	1684:1696	The remarkable capability of genus Bacteroides to use complex recalcitrant glycans may be the main reason for the decreased ability of glycan degradation in the PKU group.
34765476	6	51	from	level	1049:1053	arg1	group					1100:1104	the PKU group	1092:1104	the PKU group	1092:1104	Further, a significantly decreased level of genus Bacteroidetes (P<0.0001) in the PKU group was negatively correlated with blood Phe level (P=0.014).
34765476	5	52	theme	PKU	845:847	arg1	groups					861:866	the PKU and healthy groups	841:866	groups	861:866	Phylum-level composition differed significantly between the PKU and healthy groups (Firmicutes: 44.3% vs. 43.1%; Actinobacteria: 25.9% vs. 3.3%; Bacteroidetes: 16.6% vs. 53.2%; and Proteobacteria: 10.9% vs. 0.12%, respectively).
34765476	9	53	theme	PKU	1688:1690	arg1	group					1692:1696	the PKU group	1684:1696	the PKU group	1684:1696	The remarkable capability of genus Bacteroides to use complex recalcitrant glycans may be the main reason for the decreased ability of glycan degradation in the PKU group.
34765476	2	54	with	characteristics	334:348	arg1	phenotypes					411:420	clinical phenotypes	402:420	clinical phenotypes	402:420	Our study examined gut microbiota characteristics and metabolic pathways, and their correlations with clinical phenotypes in a high-incidence population.
34765476	5	55	dep	groups	861:866	arg1	Firmicutes					869:878	Firmicutes	869:878	Firmicutes	869:878	Phylum-level composition differed significantly between the PKU and healthy groups (Firmicutes: 44.3% vs. 43.1%; Actinobacteria: 25.9% vs. 3.3%; Bacteroidetes: 16.6% vs. 53.2%; and Proteobacteria: 10.9% vs. 0.12%, respectively).
34765476	2	56	theme	clinical	402:409	arg1	phenotypes					411:420	clinical phenotypes	402:420	clinical phenotypes	402:420	Our study examined gut microbiota characteristics and metabolic pathways, and their correlations with clinical phenotypes in a high-incidence population.
34765476	0	57	theme	gut	40:42	arg1	microbiota					44:53	gut microbiota	40:53	gut microbiota	40:53	A low abundance of genus Bacteroides in gut microbiota is negatively correlated with blood phenylalanine levels in Uygur patients with phenylketonuria.
34765476	9	58	theme	complex	1581:1587	arg1	glycans					1602:1608	complex recalcitrant glycans	1581:1608	complex recalcitrant glycans	1581:1608	The remarkable capability of genus Bacteroides to use complex recalcitrant glycans may be the main reason for the decreased ability of glycan degradation in the PKU group.
34765476	1	59	with	children	263:270	arg1	PKU					294:296	PKU	294:296	PKU	294:296	BACKGROUND A low-phenylalanine (Phe) diet affects the metabolism and diversity of gut microbial communities in children with phenylketonuria (PKU).
34765476	1	59	with	children	263:270	arg1	phenylketonuria					277:291	phenylketonuria	277:291	phenylketonuria (PKU)	277:297	BACKGROUND A low-phenylalanine (Phe) diet affects the metabolism and diversity of gut microbial communities in children with phenylketonuria (PKU).
34765476	8	60	theme	blood	1510:1514	arg1	level					1520:1524	blood Phe level	1510:1524	blood Phe level	1510:1524	CONCLUSIONS Our findings revealed that genus Bacteroide was not only in extremely low abundance in the PKU group, but was also negatively correlated with blood Phe level.
34765476	8	61	theme	PKU	1459:1461	arg1	group					1463:1467	the PKU group	1455:1467	the PKU group	1455:1467	CONCLUSIONS Our findings revealed that genus Bacteroide was not only in extremely low abundance in the PKU group, but was also negatively correlated with blood Phe level.
34765476	9	62	theme	remarkable	1531:1540	arg1	reason					1626:1631	the main reason	1617:1631	the main reason for the decreased ability of glycan degradation in the PKU group	1617:1696	The remarkable capability of genus Bacteroides to use complex recalcitrant glycans may be the main reason for the decreased ability of glycan degradation in the PKU group.
34765476	9	62	theme	remarkable	1531:1540	arg1	capability					1542:1551	The remarkable capability	1527:1551	The remarkable capability of genus Bacteroides to use complex recalcitrant glycans	1527:1608	The remarkable capability of genus Bacteroides to use complex recalcitrant glycans may be the main reason for the decreased ability of glycan degradation in the PKU group.
34765476	0	63	from	levels	105:110	arg1	patients					121:128	Uygur patients	115:128	Uygur patients with phenylketonuria	115:149	A low abundance of genus Bacteroides in gut microbiota is negatively correlated with blood phenylalanine levels in Uygur patients with phenylketonuria.
34765476	3	64	dep	METHODS	454:460	arg1	assessed					465:472	assessed	465:472	assessed clinical phenotypes and gut microbiota by 16S ribosomal RNA (rRNA) sequencing	465:550	METHODS We assessed clinical phenotypes and gut microbiota by 16S ribosomal RNA (rRNA) sequencing, and performed a correlation analysis between phenotype and gut microbiota in a PKU group (n=11) and a healthy group (n=11).
34765476	3	64	dep	METHODS	454:460	arg1	performed					557:565	performed	557:565	performed a correlation analysis between phenotype and gut microbiota in a PKU group (n=11) and a healthy group (n=11)	557:674	METHODS We assessed clinical phenotypes and gut microbiota by 16S ribosomal RNA (rRNA) sequencing, and performed a correlation analysis between phenotype and gut microbiota in a PKU group (n=11) and a healthy group (n=11).
34765476	2	65	theme	microbiota	323:332	arg1	characteristics					334:348	gut microbiota characteristics	319:348	gut microbiota characteristics	319:348	Our study examined gut microbiota characteristics and metabolic pathways, and their correlations with clinical phenotypes in a high-incidence population.
34765476	8	66	theme	Phe	1516:1518	arg1	level					1520:1524	blood Phe level	1510:1524	blood Phe level	1510:1524	CONCLUSIONS Our findings revealed that genus Bacteroide was not only in extremely low abundance in the PKU group, but was also negatively correlated with blood Phe level.
34765476	3	67	theme	healthy	655:661	arg1	n=11					670:673	n=11	670:673	n=11	670:673	METHODS We assessed clinical phenotypes and gut microbiota by 16S ribosomal RNA (rRNA) sequencing, and performed a correlation analysis between phenotype and gut microbiota in a PKU group (n=11) and a healthy group (n=11).
34765476	3	67	theme	healthy	655:661	arg1	group					663:667	a healthy group	653:667	a healthy group (n=11)	653:674	METHODS We assessed clinical phenotypes and gut microbiota by 16S ribosomal RNA (rRNA) sequencing, and performed a correlation analysis between phenotype and gut microbiota in a PKU group (n=11) and a healthy group (n=11).
34765476	6	68	theme	PKU	1096:1098	arg1	group					1100:1104	the PKU group	1092:1104	the PKU group	1092:1104	Further, a significantly decreased level of genus Bacteroidetes (P<0.0001) in the PKU group was negatively correlated with blood Phe level (P=0.014).
34765476	2	69	theme	gut	319:321	arg1	characteristics					334:348	gut microbiota characteristics	319:348	gut microbiota characteristics	319:348	Our study examined gut microbiota characteristics and metabolic pathways, and their correlations with clinical phenotypes in a high-incidence population.
34765476	0	70	with	patients	121:128	arg1	phenylketonuria					135:149	phenylketonuria	135:149	phenylketonuria	135:149	A low abundance of genus Bacteroides in gut microbiota is negatively correlated with blood phenylalanine levels in Uygur patients with phenylketonuria.
34765476	1	71	from	diversity	221:229	arg1	children					263:270	children	263:270	children with phenylketonuria (PKU)	263:297	BACKGROUND A low-phenylalanine (Phe) diet affects the metabolism and diversity of gut microbial communities in children with phenylketonuria (PKU).
34765476	5	72	theme	Proteobacteria	966:979	arg1	%					986:986	Proteobacteria: 10.9%	966:986	Proteobacteria: 10.9%	966:986	Phylum-level composition differed significantly between the PKU and healthy groups (Firmicutes: 44.3% vs. 43.1%; Actinobacteria: 25.9% vs. 3.3%; Bacteroidetes: 16.6% vs. 53.2%; and Proteobacteria: 10.9% vs. 0.12%, respectively).
34765476	1	73	dep	metabolism	206:215	arg1	the					202:204	the	202:204	the	202:204	BACKGROUND A low-phenylalanine (Phe) diet affects the metabolism and diversity of gut microbial communities in children with phenylketonuria (PKU).
34765476	3	74	theme	gut	498:500	arg1	microbiota					502:511	gut microbiota	498:511	gut microbiota	498:511	METHODS We assessed clinical phenotypes and gut microbiota by 16S ribosomal RNA (rRNA) sequencing, and performed a correlation analysis between phenotype and gut microbiota in a PKU group (n=11) and a healthy group (n=11).
34765476	4	75	theme	PKU	689:691	arg1	group					693:697	The PKU group	685:697	The PKU group	685:697	RESULTS The PKU group had significantly lower microbiota diversity than the healthy group (Pshannon=0.014).
34765476	2	76	with	pathways	364:371	arg1	phenotypes					411:420	clinical phenotypes	402:420	clinical phenotypes	402:420	Our study examined gut microbiota characteristics and metabolic pathways, and their correlations with clinical phenotypes in a high-incidence population.
34765476	3	77	theme	gut	612:614	arg1	microbiota					616:625	gut microbiota	612:625	gut microbiota	612:625	METHODS We assessed clinical phenotypes and gut microbiota by 16S ribosomal RNA (rRNA) sequencing, and performed a correlation analysis between phenotype and gut microbiota in a PKU group (n=11) and a healthy group (n=11).
34765476	7	78	theme	function	1178:1185	arg1	prediction					1187:1196	The microbial function prediction	1164:1196	The microbial function prediction of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways	1164:1259	The microbial function prediction of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways exhibited a decreased ability of glycan degradation and glutamate metabolism in the PKU group.
34765476	3	79	theme	RNA	530:532	arg1	sequencing					541:550	ribosomal RNA (rRNA) sequencing	520:550	ribosomal RNA (rRNA) sequencing	520:550	METHODS We assessed clinical phenotypes and gut microbiota by 16S ribosomal RNA (rRNA) sequencing, and performed a correlation analysis between phenotype and gut microbiota in a PKU group (n=11) and a healthy group (n=11).
34765476	7	80	theme	Encyclopedia	1211:1222	arg1	prediction					1187:1196	The microbial function prediction	1164:1196	The microbial function prediction of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways	1164:1259	The microbial function prediction of the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways exhibited a decreased ability of glycan degradation and glutamate metabolism in the PKU group.
34765476	1	81	theme	gut	234:236	arg1	communities					248:258	gut microbial communities	234:258	gut microbial communities	234:258	BACKGROUND A low-phenylalanine (Phe) diet affects the metabolism and diversity of gut microbial communities in children with phenylketonuria (PKU).
32085685	0	0	theme	effective	106:114	arg1	elimination					116:126	quick and effective elimination	96:126	quick and effective elimination of bacteria	96:138	Well-water-dispersed N-trimethyl chitosan/Fe3O4 hybrid nanoparticles as peroxidase mimetics for quick and effective elimination of bacteria.
32085685	8	1	theme	PAA/TMC/PEG	1555:1565	arg1	activity					1650:1657	peroxidise-mimetic catalytic activity	1621:1657	peroxidise-mimetic catalytic activity	1621:1657	Particularly, with an external magnet, the Fe3O4@PAA/TMC/PEG NPs, combined cationic quaternary ammonium groups and peroxidise-mimetic catalytic activity, were tested for antibacterial effect by plating method.
32085685	8	1	theme	PAA/TMC/PEG	1555:1565	arg1	NPs					1567:1569	the Fe3O4@PAA/TMC/PEG NPs	1545:1569	the Fe3O4@PAA/TMC/PEG NPs	1545:1569	Particularly, with an external magnet, the Fe3O4@PAA/TMC/PEG NPs, combined cationic quaternary ammonium groups and peroxidise-mimetic catalytic activity, were tested for antibacterial effect by plating method.
32085685	8	1	theme	PAA/TMC/PEG	1555:1565	arg1	groups					1610:1615	combined cationic quaternary ammonium groups	1572:1615	combined cationic quaternary ammonium groups	1572:1615	Particularly, with an external magnet, the Fe3O4@PAA/TMC/PEG NPs, combined cationic quaternary ammonium groups and peroxidise-mimetic catalytic activity, were tested for antibacterial effect by plating method.
32085685	4	2	theme	positive	923:930	arg1	ammonium					943:950	positive quaternary ammonium	923:950	positive quaternary ammonium groups	923:957	The functionalized coating was divided in two steps, one involved a cross-linked PEG/PAA/CS middle layer to protect the nanocrystal Fe3O4 from oxidization, the other was a hydrophilic PEG/TMC outer layer improving the water dispersion, biocompatibility, as well as supplying positive quaternary ammonium groups for a potential increase of cell binding efficiency.
32085685	8	3	theme	external	1528:1535	arg1	magnet					1537:1542	an external magnet	1525:1542	an external magnet	1525:1542	Particularly, with an external magnet, the Fe3O4@PAA/TMC/PEG NPs, combined cationic quaternary ammonium groups and peroxidise-mimetic catalytic activity, were tested for antibacterial effect by plating method.
32085685	3	4	theme	improved	532:539	arg1	dispersibility					547:560	improved water dispersibility	532:560	improved water dispersibility	532:560	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	8	5	theme	quaternary	1590:1599	arg1	NPs					1567:1569	the Fe3O4@PAA/TMC/PEG NPs	1545:1569	the Fe3O4@PAA/TMC/PEG NPs	1545:1569	Particularly, with an external magnet, the Fe3O4@PAA/TMC/PEG NPs, combined cationic quaternary ammonium groups and peroxidise-mimetic catalytic activity, were tested for antibacterial effect by plating method.
32085685	8	5	theme	quaternary	1590:1599	arg1	groups					1610:1615	combined cationic quaternary ammonium groups	1572:1615	combined cationic quaternary ammonium groups	1572:1615	Particularly, with an external magnet, the Fe3O4@PAA/TMC/PEG NPs, combined cationic quaternary ammonium groups and peroxidise-mimetic catalytic activity, were tested for antibacterial effect by plating method.
32085685	5	6	dep	structure	1016:1024	arg1	The					1012:1014	The	1012:1014	The	1012:1014	The structure, composition and morphology of Fe3O4@PAA/TMC/PEG NPs were characterized by TEM, FT-IR spectroscopy, DLS, zeta potential measurement, respectively.
32085685	8	7	theme	combined	1572:1579	arg1	NPs					1567:1569	the Fe3O4@PAA/TMC/PEG NPs	1545:1569	the Fe3O4@PAA/TMC/PEG NPs	1545:1569	Particularly, with an external magnet, the Fe3O4@PAA/TMC/PEG NPs, combined cationic quaternary ammonium groups and peroxidise-mimetic catalytic activity, were tested for antibacterial effect by plating method.
32085685	8	7	theme	combined	1572:1579	arg1	groups					1610:1615	combined cationic quaternary ammonium groups	1572:1615	combined cationic quaternary ammonium groups	1572:1615	Particularly, with an external magnet, the Fe3O4@PAA/TMC/PEG NPs, combined cationic quaternary ammonium groups and peroxidise-mimetic catalytic activity, were tested for antibacterial effect by plating method.
32085685	8	8	theme	@	1554:1554	arg1	activity					1650:1657	peroxidise-mimetic catalytic activity	1621:1657	peroxidise-mimetic catalytic activity	1621:1657	Particularly, with an external magnet, the Fe3O4@PAA/TMC/PEG NPs, combined cationic quaternary ammonium groups and peroxidise-mimetic catalytic activity, were tested for antibacterial effect by plating method.
32085685	8	8	theme	@	1554:1554	arg1	NPs					1567:1569	the Fe3O4@PAA/TMC/PEG NPs	1545:1569	the Fe3O4@PAA/TMC/PEG NPs	1545:1569	Particularly, with an external magnet, the Fe3O4@PAA/TMC/PEG NPs, combined cationic quaternary ammonium groups and peroxidise-mimetic catalytic activity, were tested for antibacterial effect by plating method.
32085685	8	8	theme	@	1554:1554	arg1	groups					1610:1615	combined cationic quaternary ammonium groups	1572:1615	combined cationic quaternary ammonium groups	1572:1615	Particularly, with an external magnet, the Fe3O4@PAA/TMC/PEG NPs, combined cationic quaternary ammonium groups and peroxidise-mimetic catalytic activity, were tested for antibacterial effect by plating method.
32085685	3	9	theme	water	541:545	arg1	dispersibility					547:560	improved water dispersibility	532:560	improved water dispersibility	532:560	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	2	10	from	decrease	346:353	arg1	peroxidase-activity					358:376	peroxidase-activity	358:376	peroxidase-activity	358:376	However, the functionalization on Fe3O4 nanoparticles always goes with the loss of superparamagnetism and decrease in peroxidase-activity.
32085685	5	11	theme	Fe3O4	1057:1061	arg1	NPs					1075:1077	Fe3O4@PAA/TMC/PEG NPs	1057:1077	Fe3O4@PAA/TMC/PEG NPs	1057:1077	The structure, composition and morphology of Fe3O4@PAA/TMC/PEG NPs were characterized by TEM, FT-IR spectroscopy, DLS, zeta potential measurement, respectively.
32085685	4	12	theme	ammonium	943:950	arg1	groups					952:957	positive quaternary ammonium groups	923:957	positive quaternary ammonium groups	923:957	The functionalized coating was divided in two steps, one involved a cross-linked PEG/PAA/CS middle layer to protect the nanocrystal Fe3O4 from oxidization, the other was a hydrophilic PEG/TMC outer layer improving the water dispersion, biocompatibility, as well as supplying positive quaternary ammonium groups for a potential increase of cell binding efficiency.
32085685	7	13	theme	bacterial	1427:1435	arg1	pathogens					1437:1445	the bacterial pathogens	1423:1445	the bacterial pathogens	1423:1445	The magnetic property of the as-prepared hybrid nanoparticles was first confirmed by VSM, and then proved by the bacterial pathogens adsorption, especially at ultralow pathogen concentration.
32085685	3	14	theme	high	583:586	arg1	magnetization					599:611	high saturation magnetization	583:611	high saturation magnetization	583:611	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	4	15	theme	functionalized	652:665	arg1	coating					667:673	The functionalized coating	648:673	The functionalized coating	648:673	The functionalized coating was divided in two steps, one involved a cross-linked PEG/PAA/CS middle layer to protect the nanocrystal Fe3O4 from oxidization, the other was a hydrophilic PEG/TMC outer layer improving the water dispersion, biocompatibility, as well as supplying positive quaternary ammonium groups for a potential increase of cell binding efficiency.
32085685	3	16	theme	green	400:404	arg1	PEG					427:429	PEG	427:429	PEG	427:429	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	3	16	theme	green	400:404	arg1	glycol					419:424	green polyethylene glycol	400:424	green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity	400:645	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	5	17	theme	potential	1136:1144	arg1	TEM					1101:1103	TEM	1101:1103	TEM	1101:1103	The structure, composition and morphology of Fe3O4@PAA/TMC/PEG NPs were characterized by TEM, FT-IR spectroscopy, DLS, zeta potential measurement, respectively.
32085685	5	17	theme	potential	1136:1144	arg1	measurement					1146:1156	zeta potential measurement	1131:1156	zeta potential measurement	1131:1156	The structure, composition and morphology of Fe3O4@PAA/TMC/PEG NPs were characterized by TEM, FT-IR spectroscopy, DLS, zeta potential measurement, respectively.
32085685	4	18	theme	quaternary	932:941	arg1	ammonium					943:950	positive quaternary ammonium	923:950	positive quaternary ammonium groups	923:957	The functionalized coating was divided in two steps, one involved a cross-linked PEG/PAA/CS middle layer to protect the nanocrystal Fe3O4 from oxidization, the other was a hydrophilic PEG/TMC outer layer improving the water dispersion, biocompatibility, as well as supplying positive quaternary ammonium groups for a potential increase of cell binding efficiency.
32085685	3	19	theme	saturation	588:597	arg1	magnetization					599:611	high saturation magnetization	583:611	high saturation magnetization	583:611	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	7	20	theme	ultralow	1473:1480	arg1	concentration					1491:1503	ultralow pathogen concentration	1473:1503	ultralow pathogen concentration	1473:1503	The magnetic property of the as-prepared hybrid nanoparticles was first confirmed by VSM, and then proved by the bacterial pathogens adsorption, especially at ultralow pathogen concentration.
32085685	4	21	theme	nanocrystal	768:778	arg1	Fe3O4					780:784	the nanocrystal Fe3O4	764:784	the nanocrystal Fe3O4	764:784	The functionalized coating was divided in two steps, one involved a cross-linked PEG/PAA/CS middle layer to protect the nanocrystal Fe3O4 from oxidization, the other was a hydrophilic PEG/TMC outer layer improving the water dispersion, biocompatibility, as well as supplying positive quaternary ammonium groups for a potential increase of cell binding efficiency.
32085685	3	22	theme	@	509:509	arg1	NPs					522:524	Fe3O4@PAA/TMC/PEG NPs	504:524	Fe3O4@PAA/TMC/PEG NPs	504:524	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	3	22	theme	@	509:509	arg1	nanoparticles					489:501	green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles	400:501	green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity	400:645	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	4	23	theme	PEG/TMC	832:838	arg1	other					808:812	other	808:812	other	808:812	The functionalized coating was divided in two steps, one involved a cross-linked PEG/PAA/CS middle layer to protect the nanocrystal Fe3O4 from oxidization, the other was a hydrophilic PEG/TMC outer layer improving the water dispersion, biocompatibility, as well as supplying positive quaternary ammonium groups for a potential increase of cell binding efficiency.
32085685	4	23	theme	PEG/TMC	832:838	arg1	layer					846:850	a hydrophilic PEG/TMC outer layer	818:850	a hydrophilic PEG/TMC outer layer improving the water dispersion, biocompatibility, as well as supplying positive quaternary ammonium groups for a potential increase of cell binding efficiency	818:1009	The functionalized coating was divided in two steps, one involved a cross-linked PEG/PAA/CS middle layer to protect the nanocrystal Fe3O4 from oxidization, the other was a hydrophilic PEG/TMC outer layer improving the water dispersion, biocompatibility, as well as supplying positive quaternary ammonium groups for a potential increase of cell binding efficiency.
32085685	0	24	theme	bacteria	131:138	arg1	elimination					116:126	quick and effective elimination	96:126	quick and effective elimination of bacteria	96:138	Well-water-dispersed N-trimethyl chitosan/Fe3O4 hybrid nanoparticles as peroxidase mimetics for quick and effective elimination of bacteria.
32085685	4	25	theme	hydrophilic	820:830	arg1	other					808:812	other	808:812	other	808:812	The functionalized coating was divided in two steps, one involved a cross-linked PEG/PAA/CS middle layer to protect the nanocrystal Fe3O4 from oxidization, the other was a hydrophilic PEG/TMC outer layer improving the water dispersion, biocompatibility, as well as supplying positive quaternary ammonium groups for a potential increase of cell binding efficiency.
32085685	4	25	theme	hydrophilic	820:830	arg1	layer					846:850	a hydrophilic PEG/TMC outer layer	818:850	a hydrophilic PEG/TMC outer layer improving the water dispersion, biocompatibility, as well as supplying positive quaternary ammonium groups for a potential increase of cell binding efficiency	818:1009	The functionalized coating was divided in two steps, one involved a cross-linked PEG/PAA/CS middle layer to protect the nanocrystal Fe3O4 from oxidization, the other was a hydrophilic PEG/TMC outer layer improving the water dispersion, biocompatibility, as well as supplying positive quaternary ammonium groups for a potential increase of cell binding efficiency.
32085685	2	26	from	functionalization	253:269	arg1	nanoparticles					280:292	Fe3O4 nanoparticles	274:292	Fe3O4 nanoparticles	274:292	However, the functionalization on Fe3O4 nanoparticles always goes with the loss of superparamagnetism and decrease in peroxidase-activity.
32085685	3	27	theme	hybrid	482:487	arg1	NPs					522:524	Fe3O4@PAA/TMC/PEG NPs	504:524	Fe3O4@PAA/TMC/PEG NPs	504:524	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	3	27	theme	hybrid	482:487	arg1	nanoparticles					489:501	green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles	400:501	green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity	400:645	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	0	28	theme	N-trimethyl	21:31	arg1	hybrid					48:53	Well-water-dispersed N-trimethyl chitosan/Fe3O4 hybrid	0:53	Well-water-dispersed N-trimethyl chitosan/Fe3O4 hybrid	0:53	Well-water-dispersed N-trimethyl chitosan/Fe3O4 hybrid nanoparticles as peroxidase mimetics for quick and effective elimination of bacteria.
32085685	8	29	theme	ammonium	1601:1608	arg1	NPs					1567:1569	the Fe3O4@PAA/TMC/PEG NPs	1545:1569	the Fe3O4@PAA/TMC/PEG NPs	1545:1569	Particularly, with an external magnet, the Fe3O4@PAA/TMC/PEG NPs, combined cationic quaternary ammonium groups and peroxidise-mimetic catalytic activity, were tested for antibacterial effect by plating method.
32085685	8	29	theme	ammonium	1601:1608	arg1	groups					1610:1615	combined cationic quaternary ammonium groups	1572:1615	combined cationic quaternary ammonium groups	1572:1615	Particularly, with an external magnet, the Fe3O4@PAA/TMC/PEG NPs, combined cationic quaternary ammonium groups and peroxidise-mimetic catalytic activity, were tested for antibacterial effect by plating method.
32085685	3	30	theme	-functionalized	431:445	arg1	NPs					522:524	Fe3O4@PAA/TMC/PEG NPs	504:524	Fe3O4@PAA/TMC/PEG NPs	504:524	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	3	30	theme	-functionalized	431:445	arg1	nanoparticles					489:501	green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles	400:501	green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity	400:645	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	8	31	theme	cationic	1581:1588	arg1	NPs					1567:1569	the Fe3O4@PAA/TMC/PEG NPs	1545:1569	the Fe3O4@PAA/TMC/PEG NPs	1545:1569	Particularly, with an external magnet, the Fe3O4@PAA/TMC/PEG NPs, combined cationic quaternary ammonium groups and peroxidise-mimetic catalytic activity, were tested for antibacterial effect by plating method.
32085685	8	31	theme	cationic	1581:1588	arg1	groups					1610:1615	combined cationic quaternary ammonium groups	1572:1615	combined cationic quaternary ammonium groups	1572:1615	Particularly, with an external magnet, the Fe3O4@PAA/TMC/PEG NPs, combined cationic quaternary ammonium groups and peroxidise-mimetic catalytic activity, were tested for antibacterial effect by plating method.
32085685	4	32	theme	PEG/PAA/CS	729:738	arg1	layer					747:751	a cross-linked PEG/PAA/CS middle layer	714:751	a cross-linked PEG/PAA/CS middle layer	714:751	The functionalized coating was divided in two steps, one involved a cross-linked PEG/PAA/CS middle layer to protect the nanocrystal Fe3O4 from oxidization, the other was a hydrophilic PEG/TMC outer layer improving the water dispersion, biocompatibility, as well as supplying positive quaternary ammonium groups for a potential increase of cell binding efficiency.
32085685	0	33	theme	Well-water-dispersed	0:19	arg1	hybrid					48:53	Well-water-dispersed N-trimethyl chitosan/Fe3O4 hybrid	0:53	Well-water-dispersed N-trimethyl chitosan/Fe3O4 hybrid	0:53	Well-water-dispersed N-trimethyl chitosan/Fe3O4 hybrid nanoparticles as peroxidase mimetics for quick and effective elimination of bacteria.
32085685	8	34	theme	peroxidise-mimetic	1621:1638	arg1	activity					1650:1657	peroxidise-mimetic catalytic activity	1621:1657	peroxidise-mimetic catalytic activity	1621:1657	Particularly, with an external magnet, the Fe3O4@PAA/TMC/PEG NPs, combined cationic quaternary ammonium groups and peroxidise-mimetic catalytic activity, were tested for antibacterial effect by plating method.
32085685	8	34	theme	peroxidise-mimetic	1621:1638	arg1	NPs					1567:1569	the Fe3O4@PAA/TMC/PEG NPs	1545:1569	the Fe3O4@PAA/TMC/PEG NPs	1545:1569	Particularly, with an external magnet, the Fe3O4@PAA/TMC/PEG NPs, combined cationic quaternary ammonium groups and peroxidise-mimetic catalytic activity, were tested for antibacterial effect by plating method.
32085685	3	35	theme	peroxidase-like	622:636	arg1	activity					638:645	well peroxidase-like activity	617:645	well peroxidase-like activity	617:645	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	3	36	theme	CS	478:479	arg1	NPs					522:524	Fe3O4@PAA/TMC/PEG NPs	504:524	Fe3O4@PAA/TMC/PEG NPs	504:524	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	3	36	theme	CS	478:479	arg1	nanoparticles					489:501	green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles	400:501	green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity	400:645	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	3	37	theme	polyethylene	406:417	arg1	PEG					427:429	PEG	427:429	PEG	427:429	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	3	37	theme	polyethylene	406:417	arg1	glycol					419:424	green polyethylene glycol	400:424	green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity	400:645	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	5	38	theme	@	1062:1062	arg1	NPs					1075:1077	Fe3O4@PAA/TMC/PEG NPs	1057:1077	Fe3O4@PAA/TMC/PEG NPs	1057:1077	The structure, composition and morphology of Fe3O4@PAA/TMC/PEG NPs were characterized by TEM, FT-IR spectroscopy, DLS, zeta potential measurement, respectively.
32085685	3	39	with	nanoparticles	489:501	arg1	activity					638:645	well peroxidase-like activity	617:645	well peroxidase-like activity	617:645	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	3	39	with	nanoparticles	489:501	arg1	dispersibility					547:560	improved water dispersibility	532:560	improved water dispersibility	532:560	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	3	39	with	nanoparticles	489:501	arg1	superparamagnetism					563:580	superparamagnetism	563:580	superparamagnetism	563:580	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	3	39	with	nanoparticles	489:501	arg1	magnetization					599:611	high saturation magnetization	583:611	high saturation magnetization	583:611	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	2	40	theme	superparamagnetism	323:340	arg1	loss					315:318	the loss	311:318	the loss of superparamagnetism	311:340	However, the functionalization on Fe3O4 nanoparticles always goes with the loss of superparamagnetism and decrease in peroxidase-activity.
32085685	2	40	theme	superparamagnetism	323:340	arg1	decrease					346:353	decrease	346:353	decrease in peroxidase-activity	346:376	However, the functionalization on Fe3O4 nanoparticles always goes with the loss of superparamagnetism and decrease in peroxidase-activity.
32085685	3	41	theme	Fe3O4	504:508	arg1	NPs					522:524	Fe3O4@PAA/TMC/PEG NPs	504:524	Fe3O4@PAA/TMC/PEG NPs	504:524	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	3	41	theme	Fe3O4	504:508	arg1	nanoparticles					489:501	green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles	400:501	green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity	400:645	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	3	42	theme	glycol	419:424	arg1	NPs					522:524	Fe3O4@PAA/TMC/PEG NPs	504:524	Fe3O4@PAA/TMC/PEG NPs	504:524	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	3	42	theme	glycol	419:424	arg1	nanoparticles					489:501	green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles	400:501	green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity	400:645	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	6	43	theme	mimics	1243:1248	arg1	activity					1250:1257	the peroxidase-like mimics activity	1223:1257	the peroxidase-like mimics activity	1223:1257	Thermal performance was characterized by TGA, and the peroxidase-like mimics activity was tested by TMB·2HCl colour development experiments.
32085685	1	44	theme	biomedical	222:231	arg1	field					233:237	the biomedical field	218:237	the biomedical field	218:237	Fe3O4 nanoparticles, used as peroxidase mimetics, exhibit splendid future in the biomedical field.
32085685	3	45	theme	magnetic/N-trimethyl	447:466	arg1	NPs					522:524	Fe3O4@PAA/TMC/PEG NPs	504:524	Fe3O4@PAA/TMC/PEG NPs	504:524	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	3	45	theme	magnetic/N-trimethyl	447:466	arg1	nanoparticles					489:501	green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles	400:501	green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity	400:645	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	8	46	theme	catalytic	1640:1648	arg1	activity					1650:1657	peroxidise-mimetic catalytic activity	1621:1657	peroxidise-mimetic catalytic activity	1621:1657	Particularly, with an external magnet, the Fe3O4@PAA/TMC/PEG NPs, combined cationic quaternary ammonium groups and peroxidise-mimetic catalytic activity, were tested for antibacterial effect by plating method.
32085685	8	46	theme	catalytic	1640:1648	arg1	NPs					1567:1569	the Fe3O4@PAA/TMC/PEG NPs	1545:1569	the Fe3O4@PAA/TMC/PEG NPs	1545:1569	Particularly, with an external magnet, the Fe3O4@PAA/TMC/PEG NPs, combined cationic quaternary ammonium groups and peroxidise-mimetic catalytic activity, were tested for antibacterial effect by plating method.
32085685	4	47	theme	binding	992:998	arg1	efficiency					1000:1009	cell binding efficiency	987:1009	cell binding efficiency	987:1009	The functionalized coating was divided in two steps, one involved a cross-linked PEG/PAA/CS middle layer to protect the nanocrystal Fe3O4 from oxidization, the other was a hydrophilic PEG/TMC outer layer improving the water dispersion, biocompatibility, as well as supplying positive quaternary ammonium groups for a potential increase of cell binding efficiency.
32085685	5	48	theme	NPs	1075:1077	arg1	composition					1027:1037	composition	1027:1037	composition	1027:1037	The structure, composition and morphology of Fe3O4@PAA/TMC/PEG NPs were characterized by TEM, FT-IR spectroscopy, DLS, zeta potential measurement, respectively.
32085685	5	48	theme	NPs	1075:1077	arg1	structure					1016:1024	structure	1016:1024	structure	1016:1024	The structure, composition and morphology of Fe3O4@PAA/TMC/PEG NPs were characterized by TEM, FT-IR spectroscopy, DLS, zeta potential measurement, respectively.
32085685	5	48	theme	NPs	1075:1077	arg1	morphology					1043:1052	morphology	1043:1052	morphology	1043:1052	The structure, composition and morphology of Fe3O4@PAA/TMC/PEG NPs were characterized by TEM, FT-IR spectroscopy, DLS, zeta potential measurement, respectively.
32085685	4	49	theme	cross-linked	716:727	arg1	layer					747:751	a cross-linked PEG/PAA/CS middle layer	714:751	a cross-linked PEG/PAA/CS middle layer	714:751	The functionalized coating was divided in two steps, one involved a cross-linked PEG/PAA/CS middle layer to protect the nanocrystal Fe3O4 from oxidization, the other was a hydrophilic PEG/TMC outer layer improving the water dispersion, biocompatibility, as well as supplying positive quaternary ammonium groups for a potential increase of cell binding efficiency.
32085685	7	50	theme	nanoparticles	1362:1374	arg1	property					1327:1334	The magnetic property	1314:1334	The magnetic property of the as-prepared hybrid nanoparticles	1314:1374	The magnetic property of the as-prepared hybrid nanoparticles was first confirmed by VSM, and then proved by the bacterial pathogens adsorption, especially at ultralow pathogen concentration.
32085685	3	51	theme	chitosan	468:475	arg1	NPs					522:524	Fe3O4@PAA/TMC/PEG NPs	504:524	Fe3O4@PAA/TMC/PEG NPs	504:524	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	3	51	theme	chitosan	468:475	arg1	nanoparticles					489:501	green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles	400:501	green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity	400:645	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	4	52	theme	cell	987:990	arg1	efficiency					1000:1009	cell binding efficiency	987:1009	cell binding efficiency	987:1009	The functionalized coating was divided in two steps, one involved a cross-linked PEG/PAA/CS middle layer to protect the nanocrystal Fe3O4 from oxidization, the other was a hydrophilic PEG/TMC outer layer improving the water dispersion, biocompatibility, as well as supplying positive quaternary ammonium groups for a potential increase of cell binding efficiency.
32085685	6	53	theme	peroxidase-like	1227:1241	arg1	activity					1250:1257	the peroxidase-like mimics activity	1223:1257	the peroxidase-like mimics activity	1223:1257	Thermal performance was characterized by TGA, and the peroxidase-like mimics activity was tested by TMB·2HCl colour development experiments.
32085685	4	54	link	cross-linked	716:727	arg1	layer					747:751	a cross-linked PEG/PAA/CS middle layer	714:751	a cross-linked PEG/PAA/CS middle layer	714:751	The functionalized coating was divided in two steps, one involved a cross-linked PEG/PAA/CS middle layer to protect the nanocrystal Fe3O4 from oxidization, the other was a hydrophilic PEG/TMC outer layer improving the water dispersion, biocompatibility, as well as supplying positive quaternary ammonium groups for a potential increase of cell binding efficiency.
32085685	8	55	theme	antibacterial	1676:1688	arg1	effect					1690:1695	antibacterial effect	1676:1695	antibacterial effect	1676:1695	Particularly, with an external magnet, the Fe3O4@PAA/TMC/PEG NPs, combined cationic quaternary ammonium groups and peroxidise-mimetic catalytic activity, were tested for antibacterial effect by plating method.
32085685	5	56	theme	zeta	1131:1134	arg1	TEM					1101:1103	TEM	1101:1103	TEM	1101:1103	The structure, composition and morphology of Fe3O4@PAA/TMC/PEG NPs were characterized by TEM, FT-IR spectroscopy, DLS, zeta potential measurement, respectively.
32085685	5	56	theme	zeta	1131:1134	arg1	measurement					1146:1156	zeta potential measurement	1131:1156	zeta potential measurement	1131:1156	The structure, composition and morphology of Fe3O4@PAA/TMC/PEG NPs were characterized by TEM, FT-IR spectroscopy, DLS, zeta potential measurement, respectively.
32085685	6	57	theme	colour	1282:1287	arg1	experiments					1301:1311	TMB·2HCl colour development experiments	1273:1311	TMB·2HCl colour development experiments	1273:1311	Thermal performance was characterized by TGA, and the peroxidase-like mimics activity was tested by TMB·2HCl colour development experiments.
32085685	1	58	theme	Fe3O4	141:145	arg1	nanoparticles					147:159	Fe3O4 nanoparticles	141:159	Fe3O4 nanoparticles	141:159	Fe3O4 nanoparticles, used as peroxidase mimetics, exhibit splendid future in the biomedical field.
32085685	0	59	theme	peroxidase	72:81	arg1	mimetics					83:90	peroxidase mimetics	72:90	peroxidase mimetics for quick and effective elimination of bacteria	72:138	Well-water-dispersed N-trimethyl chitosan/Fe3O4 hybrid nanoparticles as peroxidase mimetics for quick and effective elimination of bacteria.
32085685	6	60	theme	development	1289:1299	arg1	experiments					1301:1311	TMB·2HCl colour development experiments	1273:1311	TMB·2HCl colour development experiments	1273:1311	Thermal performance was characterized by TGA, and the peroxidase-like mimics activity was tested by TMB·2HCl colour development experiments.
32085685	6	61	theme	TMB·2HCl	1273:1280	arg1	experiments					1301:1311	TMB·2HCl colour development experiments	1273:1311	TMB·2HCl colour development experiments	1273:1311	Thermal performance was characterized by TGA, and the peroxidase-like mimics activity was tested by TMB·2HCl colour development experiments.
32085685	7	62	theme	as-prepared	1343:1353	arg1	nanoparticles					1362:1374	the as-prepared hybrid nanoparticles	1339:1374	the as-prepared hybrid nanoparticles	1339:1374	The magnetic property of the as-prepared hybrid nanoparticles was first confirmed by VSM, and then proved by the bacterial pathogens adsorption, especially at ultralow pathogen concentration.
32085685	7	63	theme	hybrid	1355:1360	arg1	nanoparticles					1362:1374	the as-prepared hybrid nanoparticles	1339:1374	the as-prepared hybrid nanoparticles	1339:1374	The magnetic property of the as-prepared hybrid nanoparticles was first confirmed by VSM, and then proved by the bacterial pathogens adsorption, especially at ultralow pathogen concentration.
32085685	5	64	theme	PAA/TMC/PEG	1063:1073	arg1	NPs					1075:1077	Fe3O4@PAA/TMC/PEG NPs	1057:1077	Fe3O4@PAA/TMC/PEG NPs	1057:1077	The structure, composition and morphology of Fe3O4@PAA/TMC/PEG NPs were characterized by TEM, FT-IR spectroscopy, DLS, zeta potential measurement, respectively.
32085685	4	65	theme	potential	965:973	arg1	increase					975:982	a potential increase	963:982	a potential increase of cell binding efficiency	963:1009	The functionalized coating was divided in two steps, one involved a cross-linked PEG/PAA/CS middle layer to protect the nanocrystal Fe3O4 from oxidization, the other was a hydrophilic PEG/TMC outer layer improving the water dispersion, biocompatibility, as well as supplying positive quaternary ammonium groups for a potential increase of cell binding efficiency.
32085685	4	66	theme	water	866:870	arg1	dispersion					872:881	the water dispersion	862:881	the water dispersion	862:881	The functionalized coating was divided in two steps, one involved a cross-linked PEG/PAA/CS middle layer to protect the nanocrystal Fe3O4 from oxidization, the other was a hydrophilic PEG/TMC outer layer improving the water dispersion, biocompatibility, as well as supplying positive quaternary ammonium groups for a potential increase of cell binding efficiency.
32085685	3	67	theme	PAA/TMC/PEG	510:520	arg1	NPs					522:524	Fe3O4@PAA/TMC/PEG NPs	504:524	Fe3O4@PAA/TMC/PEG NPs	504:524	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	3	67	theme	PAA/TMC/PEG	510:520	arg1	nanoparticles					489:501	green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles	400:501	green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity	400:645	Here, we synthesized green polyethylene glycol (PEG)-functionalized magnetic/N-trimethyl chitosan (CS) hybrid nanoparticles (Fe3O4@PAA/TMC/PEG NPs) with improved water dispersibility, superparamagnetism, high saturation magnetization and well peroxidase-like activity.
32085685	7	68	theme	pathogen	1482:1489	arg1	concentration					1491:1503	ultralow pathogen concentration	1473:1503	ultralow pathogen concentration	1473:1503	The magnetic property of the as-prepared hybrid nanoparticles was first confirmed by VSM, and then proved by the bacterial pathogens adsorption, especially at ultralow pathogen concentration.
32085685	5	69	theme	FT-IR	1106:1110	arg1	TEM					1101:1103	TEM	1101:1103	TEM	1101:1103	The structure, composition and morphology of Fe3O4@PAA/TMC/PEG NPs were characterized by TEM, FT-IR spectroscopy, DLS, zeta potential measurement, respectively.
32085685	5	69	theme	FT-IR	1106:1110	arg1	spectroscopy					1112:1123	FT-IR spectroscopy	1106:1123	FT-IR spectroscopy	1106:1123	The structure, composition and morphology of Fe3O4@PAA/TMC/PEG NPs were characterized by TEM, FT-IR spectroscopy, DLS, zeta potential measurement, respectively.
32085685	4	70	theme	outer	840:844	arg1	other					808:812	other	808:812	other	808:812	The functionalized coating was divided in two steps, one involved a cross-linked PEG/PAA/CS middle layer to protect the nanocrystal Fe3O4 from oxidization, the other was a hydrophilic PEG/TMC outer layer improving the water dispersion, biocompatibility, as well as supplying positive quaternary ammonium groups for a potential increase of cell binding efficiency.
32085685	4	70	theme	outer	840:844	arg1	layer					846:850	a hydrophilic PEG/TMC outer layer	818:850	a hydrophilic PEG/TMC outer layer improving the water dispersion, biocompatibility, as well as supplying positive quaternary ammonium groups for a potential increase of cell binding efficiency	818:1009	The functionalized coating was divided in two steps, one involved a cross-linked PEG/PAA/CS middle layer to protect the nanocrystal Fe3O4 from oxidization, the other was a hydrophilic PEG/TMC outer layer improving the water dispersion, biocompatibility, as well as supplying positive quaternary ammonium groups for a potential increase of cell binding efficiency.
32085685	4	71	theme	middle	740:745	arg1	layer					747:751	a cross-linked PEG/PAA/CS middle layer	714:751	a cross-linked PEG/PAA/CS middle layer	714:751	The functionalized coating was divided in two steps, one involved a cross-linked PEG/PAA/CS middle layer to protect the nanocrystal Fe3O4 from oxidization, the other was a hydrophilic PEG/TMC outer layer improving the water dispersion, biocompatibility, as well as supplying positive quaternary ammonium groups for a potential increase of cell binding efficiency.
32085685	2	72	from	loss	315:318	arg1	peroxidase-activity					358:376	peroxidase-activity	358:376	peroxidase-activity	358:376	However, the functionalization on Fe3O4 nanoparticles always goes with the loss of superparamagnetism and decrease in peroxidase-activity.
32085685	6	73	theme	Thermal	1173:1179	arg1	performance					1181:1191	Thermal performance	1173:1191	Thermal performance	1173:1191	Thermal performance was characterized by TGA, and the peroxidase-like mimics activity was tested by TMB·2HCl colour development experiments.
32085685	0	74	theme	quick	96:100	arg1	elimination					116:126	quick and effective elimination	96:126	quick and effective elimination of bacteria	96:138	Well-water-dispersed N-trimethyl chitosan/Fe3O4 hybrid nanoparticles as peroxidase mimetics for quick and effective elimination of bacteria.
32085685	8	75	theme	Fe3O4	1549:1553	arg1	activity					1650:1657	peroxidise-mimetic catalytic activity	1621:1657	peroxidise-mimetic catalytic activity	1621:1657	Particularly, with an external magnet, the Fe3O4@PAA/TMC/PEG NPs, combined cationic quaternary ammonium groups and peroxidise-mimetic catalytic activity, were tested for antibacterial effect by plating method.
32085685	8	75	theme	Fe3O4	1549:1553	arg1	NPs					1567:1569	the Fe3O4@PAA/TMC/PEG NPs	1545:1569	the Fe3O4@PAA/TMC/PEG NPs	1545:1569	Particularly, with an external magnet, the Fe3O4@PAA/TMC/PEG NPs, combined cationic quaternary ammonium groups and peroxidise-mimetic catalytic activity, were tested for antibacterial effect by plating method.
32085685	8	75	theme	Fe3O4	1549:1553	arg1	groups					1610:1615	combined cationic quaternary ammonium groups	1572:1615	combined cationic quaternary ammonium groups	1572:1615	Particularly, with an external magnet, the Fe3O4@PAA/TMC/PEG NPs, combined cationic quaternary ammonium groups and peroxidise-mimetic catalytic activity, were tested for antibacterial effect by plating method.
32085685	2	76	theme	Fe3O4	274:278	arg1	nanoparticles					280:292	Fe3O4 nanoparticles	274:292	Fe3O4 nanoparticles	274:292	However, the functionalization on Fe3O4 nanoparticles always goes with the loss of superparamagnetism and decrease in peroxidase-activity.
32085685	7	77	theme	magnetic	1318:1325	arg1	property					1327:1334	The magnetic property	1314:1334	The magnetic property of the as-prepared hybrid nanoparticles	1314:1374	The magnetic property of the as-prepared hybrid nanoparticles was first confirmed by VSM, and then proved by the bacterial pathogens adsorption, especially at ultralow pathogen concentration.
32085685	0	78	theme	chitosan/Fe3O4	33:46	arg1	hybrid					48:53	Well-water-dispersed N-trimethyl chitosan/Fe3O4 hybrid	0:53	Well-water-dispersed N-trimethyl chitosan/Fe3O4 hybrid	0:53	Well-water-dispersed N-trimethyl chitosan/Fe3O4 hybrid nanoparticles as peroxidase mimetics for quick and effective elimination of bacteria.
32085685	1	79	theme	peroxidase	170:179	arg1	mimetics					181:188	peroxidase mimetics	170:188	peroxidase mimetics	170:188	Fe3O4 nanoparticles, used as peroxidase mimetics, exhibit splendid future in the biomedical field.
32085685	4	80	theme	efficiency	1000:1009	arg1	increase					975:982	a potential increase	963:982	a potential increase of cell binding efficiency	963:1009	The functionalized coating was divided in two steps, one involved a cross-linked PEG/PAA/CS middle layer to protect the nanocrystal Fe3O4 from oxidization, the other was a hydrophilic PEG/TMC outer layer improving the water dispersion, biocompatibility, as well as supplying positive quaternary ammonium groups for a potential increase of cell binding efficiency.
33686049	3	0	theme	ribosomal	651:659	arg1	sequencing					690:699	ribosomal ribonucleic acid (rRNA) gene sequencing	651:699	ribosomal ribonucleic acid (rRNA) gene sequencing	651:699	MATERIAL AND METHODS Our study investigated therapeutic and preventive effects of B. bifidum ATCC 29521 on C57BL/6 mice with dextran sulfate sodium (DSS)-induced acute colitis via 16S ribosomal ribonucleic acid (rRNA) gene sequencing.
33686049	10	1	theme	gene	1715:1718	arg1	sequencing					1720:1729	rRNA gene sequencing	1710:1729	rRNA gene sequencing	1710:1729	CONCLUSIONS 16S rRNA gene sequencing showed that pretreatment with B. bifidum ATCC 29521 reduced intestinal inflammation and altered the gut microbiota to favor the genera Intestinimonas and Bacteroides.
33686049	1	2	theme	inflammatory	253:264	arg1	IBD					281:283	IBD	281:283	IBD	281:283	BACKGROUND Bifidobacterium is a potentially effective and safe treatment for patients with inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease.
33686049	1	2	theme	inflammatory	253:264	arg1	disease					272:278	inflammatory bowel disease	253:278	inflammatory bowel disease (IBD)	253:284	BACKGROUND Bifidobacterium is a potentially effective and safe treatment for patients with inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease.
33686049	4	3	theme	clinical	843:850	arg1	indicators					867:876	clinical and pathologic indicators	843:876	clinical and pathologic indicators	843:876	RESULTS Treatment and pretreatment of mice with B. bifidum ATCC 29521 significantly alleviated the severity of acute colitis on the basis of clinical and pathologic indicators.
33686049	3	4	theme	gene	685:688	arg1	sequencing					690:699	ribosomal ribonucleic acid (rRNA) gene sequencing	651:699	ribosomal ribonucleic acid (rRNA) gene sequencing	651:699	MATERIAL AND METHODS Our study investigated therapeutic and preventive effects of B. bifidum ATCC 29521 on C57BL/6 mice with dextran sulfate sodium (DSS)-induced acute colitis via 16S ribosomal ribonucleic acid (rRNA) gene sequencing.
33686049	10	5	theme	B.	1761:1762	arg1	ATCC					1772:1775	B. bifidum ATCC 29521	1761:1781	B. bifidum ATCC 29521	1761:1781	CONCLUSIONS 16S rRNA gene sequencing showed that pretreatment with B. bifidum ATCC 29521 reduced intestinal inflammation and altered the gut microbiota to favor the genera Intestinimonas and Bacteroides.
33686049	4	6	theme	acute	813:817	arg1	colitis					819:825	acute colitis	813:825	acute colitis	813:825	RESULTS Treatment and pretreatment of mice with B. bifidum ATCC 29521 significantly alleviated the severity of acute colitis on the basis of clinical and pathologic indicators.
33686049	5	7	with	mice	990:993	arg1	colitis					1012:1018	DSS-induced colitis	1000:1018	DSS-induced colitis in both treated and pretreated groups	1000:1056	16S rRNA gene sequencing showed that administration of B. bifidum shifted composition of the gut microbiome in mice with DSS-induced colitis in both treated and pretreated groups.
33686049	3	8	theme	rRNA	679:682	arg1	sequencing					690:699	ribosomal ribonucleic acid (rRNA) gene sequencing	651:699	ribosomal ribonucleic acid (rRNA) gene sequencing	651:699	MATERIAL AND METHODS Our study investigated therapeutic and preventive effects of B. bifidum ATCC 29521 on C57BL/6 mice with dextran sulfate sodium (DSS)-induced acute colitis via 16S ribosomal ribonucleic acid (rRNA) gene sequencing.
33686049	1	9	theme	BACKGROUND	162:171	arg1	Bifidobacterium					173:187	BACKGROUND Bifidobacterium	162:187	BACKGROUND Bifidobacterium	162:187	BACKGROUND Bifidobacterium is a potentially effective and safe treatment for patients with inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease.
33686049	1	9	theme	BACKGROUND	162:171	arg1	treatment					225:233	a potentially effective and safe treatment	192:233	a potentially effective and safe treatment for patients with inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease	192:334	BACKGROUND Bifidobacterium is a potentially effective and safe treatment for patients with inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease.
33686049	9	10	theme	bifidum-pretreated	1668:1685	arg1	group					1687:1691	the B. bifidum-pretreated group	1661:1691	the B. bifidum-pretreated group	1661:1691	Furthermore, the genera Intestinimonas and Bacteroides were significantly associated with the B. bifidum-pretreated group.
33686049	3	11	theme	ATCC	560:563	arg1	effects					538:544	therapeutic and preventive effects	511:544	therapeutic and preventive effects of B. bifidum ATCC 29521 on C57BL/6 mice with dextran sulfate sodium (DSS)-induced acute colitis	511:641	MATERIAL AND METHODS Our study investigated therapeutic and preventive effects of B. bifidum ATCC 29521 on C57BL/6 mice with dextran sulfate sodium (DSS)-induced acute colitis via 16S ribosomal ribonucleic acid (rRNA) gene sequencing.
33686049	8	12	dep	analysis	1338:1345	arg1	basis					1309:1313	basis	1309:1313	basis	1309:1313	On the basis of linear discriminant analysis of effect size, the abundance of the genus Escherichia-Shigella, belonging to the family Enterobacteriaceae, was reduced in the B. bifidum-treated group, indicating that pathogens were inhibited by the B. bifidum treatment.
33686049	8	12	dep	analysis	1338:1345	arg1	the					1305:1307	the	1305:1307	the	1305:1307	On the basis of linear discriminant analysis of effect size, the abundance of the genus Escherichia-Shigella, belonging to the family Enterobacteriaceae, was reduced in the B. bifidum-treated group, indicating that pathogens were inhibited by the B. bifidum treatment.
33686049	2	13	from	information	346:356	arg1	influence					365:373	the influence	361:373	the influence of B. bifidum on gut microbial diversity of treated and pretreated IBD patients	361:453	However, information on the influence of B. bifidum on gut microbial diversity of treated and pretreated IBD patients is limited.
33686049	3	14	theme	B.	549:550	arg1	ATCC					560:563	B. bifidum ATCC 29521	549:569	B. bifidum ATCC 29521	549:569	MATERIAL AND METHODS Our study investigated therapeutic and preventive effects of B. bifidum ATCC 29521 on C57BL/6 mice with dextran sulfate sodium (DSS)-induced acute colitis via 16S ribosomal ribonucleic acid (rRNA) gene sequencing.
33686049	0	15	theme	Colitis	119:125	arg1	Model					104:108	a Mouse Model	96:108	a Mouse Model of Acute Colitis Induced by Dextran Sulfate Sodium	96:159	Effects of Pretreatment with Bifidobacterium bifidum Using 16S Ribosomal RNA Gene Sequencing in a Mouse Model of Acute Colitis Induced by Dextran Sulfate Sodium.
33686049	4	16	from	severity	801:808	arg1	basis					834:838	the basis	830:838	the basis of clinical and pathologic indicators	830:876	RESULTS Treatment and pretreatment of mice with B. bifidum ATCC 29521 significantly alleviated the severity of acute colitis on the basis of clinical and pathologic indicators.
33686049	4	17	theme	pathologic	856:865	arg1	indicators					867:876	clinical and pathologic indicators	843:876	clinical and pathologic indicators	843:876	RESULTS Treatment and pretreatment of mice with B. bifidum ATCC 29521 significantly alleviated the severity of acute colitis on the basis of clinical and pathologic indicators.
33686049	6	18	theme	B.	1080:1081	arg1	ATCC					1091:1094	B. bifidum ATCC 29521	1080:1100	B. bifidum ATCC 29521	1080:1100	Mice pretreated with B. bifidum ATCC 29521 for 21 days exhibited a significant increase in diversity of the gut microbiome.
33686049	5	19	theme	rRNA	883:886	arg1	sequencing					893:902	16S rRNA gene sequencing	879:902	16S rRNA gene sequencing	879:902	16S rRNA gene sequencing showed that administration of B. bifidum shifted composition of the gut microbiome in mice with DSS-induced colitis in both treated and pretreated groups.
33686049	6	20	theme	microbiome	1171:1180	arg1	diversity					1150:1158	diversity	1150:1158	diversity of the gut microbiome	1150:1180	Mice pretreated with B. bifidum ATCC 29521 for 21 days exhibited a significant increase in diversity of the gut microbiome.
33686049	0	21	theme	Sulfate	146:152	arg1	Sodium					154:159	Dextran Sulfate Sodium	138:159	Dextran Sulfate Sodium	138:159	Effects of Pretreatment with Bifidobacterium bifidum Using 16S Ribosomal RNA Gene Sequencing in a Mouse Model of Acute Colitis Induced by Dextran Sulfate Sodium.
33686049	10	22	theme	bifidum	1764:1770	arg1	ATCC					1772:1775	B. bifidum ATCC 29521	1761:1781	B. bifidum ATCC 29521	1761:1781	CONCLUSIONS 16S rRNA gene sequencing showed that pretreatment with B. bifidum ATCC 29521 reduced intestinal inflammation and altered the gut microbiota to favor the genera Intestinimonas and Bacteroides.
33686049	5	23	theme	gene	888:891	arg1	sequencing					893:902	16S rRNA gene sequencing	879:902	16S rRNA gene sequencing	879:902	16S rRNA gene sequencing showed that administration of B. bifidum shifted composition of the gut microbiome in mice with DSS-induced colitis in both treated and pretreated groups.
33686049	0	24	theme	Ribosomal	63:71	arg1	Sequencing					82:91	16S Ribosomal RNA Gene Sequencing	59:91	16S Ribosomal RNA Gene Sequencing	59:91	Effects of Pretreatment with Bifidobacterium bifidum Using 16S Ribosomal RNA Gene Sequencing in a Mouse Model of Acute Colitis Induced by Dextran Sulfate Sodium.
33686049	10	25	theme	gut	1831:1833	arg1	microbiota					1835:1844	the gut microbiota	1827:1844	the gut microbiota to favor the genera Intestinimonas and Bacteroides	1827:1895	CONCLUSIONS 16S rRNA gene sequencing showed that pretreatment with B. bifidum ATCC 29521 reduced intestinal inflammation and altered the gut microbiota to favor the genera Intestinimonas and Bacteroides.
33686049	0	26	theme	Gene	77:80	arg1	Sequencing					82:91	16S Ribosomal RNA Gene Sequencing	59:91	16S Ribosomal RNA Gene Sequencing	59:91	Effects of Pretreatment with Bifidobacterium bifidum Using 16S Ribosomal RNA Gene Sequencing in a Mouse Model of Acute Colitis Induced by Dextran Sulfate Sodium.
33686049	8	27	theme	B.	1475:1476	arg1	group					1494:1498	the B. bifidum-treated group	1471:1498	the B. bifidum-treated group	1471:1498	On the basis of linear discriminant analysis of effect size, the abundance of the genus Escherichia-Shigella, belonging to the family Enterobacteriaceae, was reduced in the B. bifidum-treated group, indicating that pathogens were inhibited by the B. bifidum treatment.
33686049	3	28	from	effects	538:544	arg1	mice					582:585	C57BL/6 mice	574:585	C57BL/6 mice with dextran sulfate sodium (DSS)-induced acute colitis	574:641	MATERIAL AND METHODS Our study investigated therapeutic and preventive effects of B. bifidum ATCC 29521 on C57BL/6 mice with dextran sulfate sodium (DSS)-induced acute colitis via 16S ribosomal ribonucleic acid (rRNA) gene sequencing.
33686049	5	29	theme	bifidum	937:943	arg1	administration					916:929	administration	916:929	administration of B. bifidum	916:943	16S rRNA gene sequencing showed that administration of B. bifidum shifted composition of the gut microbiome in mice with DSS-induced colitis in both treated and pretreated groups.
33686049	8	30	theme	discriminant	1325:1336	arg1	analysis					1338:1345	linear discriminant analysis	1318:1345	linear discriminant analysis of effect size	1318:1360	On the basis of linear discriminant analysis of effect size, the abundance of the genus Escherichia-Shigella, belonging to the family Enterobacteriaceae, was reduced in the B. bifidum-treated group, indicating that pathogens were inhibited by the B. bifidum treatment.
33686049	3	31	with	mice	582:585	arg1	colitis					635:641	dextran sulfate sodium (DSS)-induced acute colitis	592:641	dextran sulfate sodium (DSS)-induced acute colitis	592:641	MATERIAL AND METHODS Our study investigated therapeutic and preventive effects of B. bifidum ATCC 29521 on C57BL/6 mice with dextran sulfate sodium (DSS)-induced acute colitis via 16S ribosomal ribonucleic acid (rRNA) gene sequencing.
33686049	10	32	with	pretreatment	1743:1754	arg1	ATCC					1772:1775	B. bifidum ATCC 29521	1761:1781	B. bifidum ATCC 29521	1761:1781	CONCLUSIONS 16S rRNA gene sequencing showed that pretreatment with B. bifidum ATCC 29521 reduced intestinal inflammation and altered the gut microbiota to favor the genera Intestinimonas and Bacteroides.
33686049	0	33	theme	RNA	73:75	arg1	Sequencing					82:91	16S Ribosomal RNA Gene Sequencing	59:91	16S Ribosomal RNA Gene Sequencing	59:91	Effects of Pretreatment with Bifidobacterium bifidum Using 16S Ribosomal RNA Gene Sequencing in a Mouse Model of Acute Colitis Induced by Dextran Sulfate Sodium.
33686049	8	34	theme	size	1357:1360	arg1	analysis					1338:1345	linear discriminant analysis	1318:1345	linear discriminant analysis of effect size	1318:1360	On the basis of linear discriminant analysis of effect size, the abundance of the genus Escherichia-Shigella, belonging to the family Enterobacteriaceae, was reduced in the B. bifidum-treated group, indicating that pathogens were inhibited by the B. bifidum treatment.
33686049	2	35	theme	microbial	396:404	arg1	diversity					406:414	gut microbial diversity	392:414	gut microbial diversity	392:414	However, information on the influence of B. bifidum on gut microbial diversity of treated and pretreated IBD patients is limited.
33686049	0	36	theme	Pretreatment	11:22	arg1	Effects					0:6	Effects	0:6	Effects of Pretreatment with Bifidobacterium bifidum	0:51	Effects of Pretreatment with Bifidobacterium bifidum Using 16S Ribosomal RNA Gene Sequencing in a Mouse Model of Acute Colitis Induced by Dextran Sulfate Sodium.
33686049	7	37	theme	time	1289:1292	arg1	points					1294:1299	time points	1289:1299	time points	1289:1299	Principal coordinate analysis showed that gut microbiota structure was shaped by different treatments and time points.
33686049	3	38	theme	-induced	620:627	arg1	colitis					635:641	dextran sulfate sodium (DSS)-induced acute colitis	592:641	dextran sulfate sodium (DSS)-induced acute colitis	592:641	MATERIAL AND METHODS Our study investigated therapeutic and preventive effects of B. bifidum ATCC 29521 on C57BL/6 mice with dextran sulfate sodium (DSS)-induced acute colitis via 16S ribosomal ribonucleic acid (rRNA) gene sequencing.
33686049	2	39	from	influence	365:373	arg1	diversity					406:414	gut microbial diversity	392:414	gut microbial diversity	392:414	However, information on the influence of B. bifidum on gut microbial diversity of treated and pretreated IBD patients is limited.
33686049	2	40	theme	IBD	442:444	arg1	patients					446:453	treated and pretreated IBD patients	419:453	treated and pretreated IBD patients	419:453	However, information on the influence of B. bifidum on gut microbial diversity of treated and pretreated IBD patients is limited.
33686049	0	41	with	Pretreatment	11:22	arg1	bifidum					45:51	Bifidobacterium bifidum	29:51	Bifidobacterium bifidum	29:51	Effects of Pretreatment with Bifidobacterium bifidum Using 16S Ribosomal RNA Gene Sequencing in a Mouse Model of Acute Colitis Induced by Dextran Sulfate Sodium.
33686049	10	42	theme	rRNA	1710:1713	arg1	sequencing					1720:1729	rRNA gene sequencing	1710:1729	rRNA gene sequencing	1710:1729	CONCLUSIONS 16S rRNA gene sequencing showed that pretreatment with B. bifidum ATCC 29521 reduced intestinal inflammation and altered the gut microbiota to favor the genera Intestinimonas and Bacteroides.
33686049	5	43	theme	DSS-induced	1000:1010	arg1	colitis					1012:1018	DSS-induced colitis	1000:1018	DSS-induced colitis in both treated and pretreated groups	1000:1056	16S rRNA gene sequencing showed that administration of B. bifidum shifted composition of the gut microbiome in mice with DSS-induced colitis in both treated and pretreated groups.
33686049	8	44	theme	bifidum	1552:1558	arg1	treatment					1560:1568	the B. bifidum treatment	1545:1568	the B. bifidum treatment	1545:1568	On the basis of linear discriminant analysis of effect size, the abundance of the genus Escherichia-Shigella, belonging to the family Enterobacteriaceae, was reduced in the B. bifidum-treated group, indicating that pathogens were inhibited by the B. bifidum treatment.
33686049	4	45	theme	bifidum	753:759	arg1	ATCC					761:764	B. bifidum ATCC 29521	750:770	B. bifidum ATCC 29521	750:770	RESULTS Treatment and pretreatment of mice with B. bifidum ATCC 29521 significantly alleviated the severity of acute colitis on the basis of clinical and pathologic indicators.
33686049	1	46	theme	safe	220:223	arg1	Bifidobacterium					173:187	BACKGROUND Bifidobacterium	162:187	BACKGROUND Bifidobacterium	162:187	BACKGROUND Bifidobacterium is a potentially effective and safe treatment for patients with inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease.
33686049	1	46	theme	safe	220:223	arg1	treatment					225:233	a potentially effective and safe treatment	192:233	a potentially effective and safe treatment for patients with inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease	192:334	BACKGROUND Bifidobacterium is a potentially effective and safe treatment for patients with inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease.
33686049	6	47	theme	bifidum	1083:1089	arg1	ATCC					1091:1094	B. bifidum ATCC 29521	1080:1100	B. bifidum ATCC 29521	1080:1100	Mice pretreated with B. bifidum ATCC 29521 for 21 days exhibited a significant increase in diversity of the gut microbiome.
33686049	2	48	theme	bifidum	381:387	arg1	influence					365:373	the influence	361:373	the influence of B. bifidum on gut microbial diversity of treated and pretreated IBD patients	361:453	However, information on the influence of B. bifidum on gut microbial diversity of treated and pretreated IBD patients is limited.
33686049	5	49	theme	microbiome	976:985	arg1	composition					953:963	composition	953:963	composition of the gut microbiome in mice with DSS-induced colitis in both treated and pretreated groups	953:1056	16S rRNA gene sequencing showed that administration of B. bifidum shifted composition of the gut microbiome in mice with DSS-induced colitis in both treated and pretreated groups.
33686049	7	50	theme	microbiota	1229:1238	arg1	structure					1240:1248	gut microbiota structure	1225:1248	gut microbiota structure	1225:1248	Principal coordinate analysis showed that gut microbiota structure was shaped by different treatments and time points.
33686049	5	51	from	composition	953:963	arg1	mice					990:993	mice	990:993	mice with DSS-induced colitis in both treated and pretreated groups	990:1056	16S rRNA gene sequencing showed that administration of B. bifidum shifted composition of the gut microbiome in mice with DSS-induced colitis in both treated and pretreated groups.
33686049	3	52	theme	ribonucleic	661:671	arg1	sequencing					690:699	ribosomal ribonucleic acid (rRNA) gene sequencing	651:699	ribosomal ribonucleic acid (rRNA) gene sequencing	651:699	MATERIAL AND METHODS Our study investigated therapeutic and preventive effects of B. bifidum ATCC 29521 on C57BL/6 mice with dextran sulfate sodium (DSS)-induced acute colitis via 16S ribosomal ribonucleic acid (rRNA) gene sequencing.
33686049	3	53	theme	preventive	527:536	arg1	effects					538:544	therapeutic and preventive effects	511:544	therapeutic and preventive effects of B. bifidum ATCC 29521 on C57BL/6 mice with dextran sulfate sodium (DSS)-induced acute colitis	511:641	MATERIAL AND METHODS Our study investigated therapeutic and preventive effects of B. bifidum ATCC 29521 on C57BL/6 mice with dextran sulfate sodium (DSS)-induced acute colitis via 16S ribosomal ribonucleic acid (rRNA) gene sequencing.
33686049	1	54	theme	bowel	266:270	arg1	IBD					281:283	IBD	281:283	IBD	281:283	BACKGROUND Bifidobacterium is a potentially effective and safe treatment for patients with inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease.
33686049	1	54	theme	bowel	266:270	arg1	disease					272:278	inflammatory bowel disease	253:278	inflammatory bowel disease (IBD)	253:284	BACKGROUND Bifidobacterium is a potentially effective and safe treatment for patients with inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease.
33686049	10	55	theme	intestinal	1791:1800	arg1	inflammation					1802:1813	intestinal inflammation	1791:1813	intestinal inflammation	1791:1813	CONCLUSIONS 16S rRNA gene sequencing showed that pretreatment with B. bifidum ATCC 29521 reduced intestinal inflammation and altered the gut microbiota to favor the genera Intestinimonas and Bacteroides.
33686049	3	56	theme	therapeutic	511:521	arg1	effects					538:544	therapeutic and preventive effects	511:544	therapeutic and preventive effects of B. bifidum ATCC 29521 on C57BL/6 mice with dextran sulfate sodium (DSS)-induced acute colitis	511:641	MATERIAL AND METHODS Our study investigated therapeutic and preventive effects of B. bifidum ATCC 29521 on C57BL/6 mice with dextran sulfate sodium (DSS)-induced acute colitis via 16S ribosomal ribonucleic acid (rRNA) gene sequencing.
33686049	9	57	theme	B.	1665:1666	arg1	group					1687:1691	the B. bifidum-pretreated group	1661:1691	the B. bifidum-pretreated group	1661:1691	Furthermore, the genera Intestinimonas and Bacteroides were significantly associated with the B. bifidum-pretreated group.
33686049	6	58	theme	significant	1126:1136	arg1	increase					1138:1145	a significant increase	1124:1145	a significant increase in diversity of the gut microbiome	1124:1180	Mice pretreated with B. bifidum ATCC 29521 for 21 days exhibited a significant increase in diversity of the gut microbiome.
33686049	7	59	theme	Principal	1183:1191	arg1	analysis					1204:1211	Principal coordinate analysis	1183:1211	Principal coordinate analysis	1183:1211	Principal coordinate analysis showed that gut microbiota structure was shaped by different treatments and time points.
33686049	3	60	theme	bifidum	552:558	arg1	ATCC					560:563	B. bifidum ATCC 29521	549:569	B. bifidum ATCC 29521	549:569	MATERIAL AND METHODS Our study investigated therapeutic and preventive effects of B. bifidum ATCC 29521 on C57BL/6 mice with dextran sulfate sodium (DSS)-induced acute colitis via 16S ribosomal ribonucleic acid (rRNA) gene sequencing.
33686049	4	61	theme	colitis	819:825	arg1	severity					801:808	the severity	797:808	the severity of acute colitis on the basis of clinical and pathologic indicators	797:876	RESULTS Treatment and pretreatment of mice with B. bifidum ATCC 29521 significantly alleviated the severity of acute colitis on the basis of clinical and pathologic indicators.
33686049	0	62	theme	Acute	113:117	arg1	Colitis					119:125	Acute Colitis	113:125	Acute Colitis Induced by Dextran Sulfate Sodium	113:159	Effects of Pretreatment with Bifidobacterium bifidum Using 16S Ribosomal RNA Gene Sequencing in a Mouse Model of Acute Colitis Induced by Dextran Sulfate Sodium.
33686049	4	63	with	pretreatment	724:735	arg1	ATCC					761:764	B. bifidum ATCC 29521	750:770	B. bifidum ATCC 29521	750:770	RESULTS Treatment and pretreatment of mice with B. bifidum ATCC 29521 significantly alleviated the severity of acute colitis on the basis of clinical and pathologic indicators.
33686049	5	64	theme	pretreated	1040:1049	arg1	groups					1051:1056	both treated and pretreated groups	1023:1056	both treated and pretreated groups	1023:1056	16S rRNA gene sequencing showed that administration of B. bifidum shifted composition of the gut microbiome in mice with DSS-induced colitis in both treated and pretreated groups.
33686049	3	65	theme	C57BL/6	574:580	arg1	mice					582:585	C57BL/6 mice	574:585	C57BL/6 mice with dextran sulfate sodium (DSS)-induced acute colitis	574:641	MATERIAL AND METHODS Our study investigated therapeutic and preventive effects of B. bifidum ATCC 29521 on C57BL/6 mice with dextran sulfate sodium (DSS)-induced acute colitis via 16S ribosomal ribonucleic acid (rRNA) gene sequencing.
33686049	1	66	theme	effective	206:214	arg1	Bifidobacterium					173:187	BACKGROUND Bifidobacterium	162:187	BACKGROUND Bifidobacterium	162:187	BACKGROUND Bifidobacterium is a potentially effective and safe treatment for patients with inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease.
33686049	1	66	theme	effective	206:214	arg1	treatment					225:233	a potentially effective and safe treatment	192:233	a potentially effective and safe treatment for patients with inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease	192:334	BACKGROUND Bifidobacterium is a potentially effective and safe treatment for patients with inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease.
33686049	8	67	theme	family	1429:1434	arg1	Enterobacteriaceae					1436:1453	the family Enterobacteriaceae	1425:1453	the family Enterobacteriaceae	1425:1453	On the basis of linear discriminant analysis of effect size, the abundance of the genus Escherichia-Shigella, belonging to the family Enterobacteriaceae, was reduced in the B. bifidum-treated group, indicating that pathogens were inhibited by the B. bifidum treatment.
33686049	4	68	with	Treatment	710:718	arg1	ATCC					761:764	B. bifidum ATCC 29521	750:770	B. bifidum ATCC 29521	750:770	RESULTS Treatment and pretreatment of mice with B. bifidum ATCC 29521 significantly alleviated the severity of acute colitis on the basis of clinical and pathologic indicators.
33686049	0	69	theme	Dextran	138:144	arg1	Sodium					154:159	Dextran Sulfate Sodium	138:159	Dextran Sulfate Sodium	138:159	Effects of Pretreatment with Bifidobacterium bifidum Using 16S Ribosomal RNA Gene Sequencing in a Mouse Model of Acute Colitis Induced by Dextran Sulfate Sodium.
33686049	5	70	theme	16S	879:881	arg1	sequencing					893:902	16S rRNA gene sequencing	879:902	16S rRNA gene sequencing	879:902	16S rRNA gene sequencing showed that administration of B. bifidum shifted composition of the gut microbiome in mice with DSS-induced colitis in both treated and pretreated groups.
33686049	8	71	theme	genus	1384:1388	arg1	Escherichia-Shigella					1390:1409	the genus Escherichia-Shigella	1380:1409	the genus Escherichia-Shigella	1380:1409	On the basis of linear discriminant analysis of effect size, the abundance of the genus Escherichia-Shigella, belonging to the family Enterobacteriaceae, was reduced in the B. bifidum-treated group, indicating that pathogens were inhibited by the B. bifidum treatment.
33686049	4	72	dep	RESULTS	702:708	arg1	alleviated					786:795	alleviated	786:795	alleviated the severity of acute colitis on the basis of clinical and pathologic indicators	786:876	RESULTS Treatment and pretreatment of mice with B. bifidum ATCC 29521 significantly alleviated the severity of acute colitis on the basis of clinical and pathologic indicators.
33686049	4	73	theme	indicators	867:876	arg1	basis					834:838	the basis	830:838	the basis of clinical and pathologic indicators	830:876	RESULTS Treatment and pretreatment of mice with B. bifidum ATCC 29521 significantly alleviated the severity of acute colitis on the basis of clinical and pathologic indicators.
33686049	6	74	theme	gut	1167:1169	arg1	microbiome					1171:1180	the gut microbiome	1163:1180	the gut microbiome	1163:1180	Mice pretreated with B. bifidum ATCC 29521 for 21 days exhibited a significant increase in diversity of the gut microbiome.
33686049	7	75	theme	coordinate	1193:1202	arg1	analysis					1204:1211	Principal coordinate analysis	1183:1211	Principal coordinate analysis	1183:1211	Principal coordinate analysis showed that gut microbiota structure was shaped by different treatments and time points.
33686049	5	76	theme	treated	1028:1034	arg1	groups					1051:1056	both treated and pretreated groups	1023:1056	both treated and pretreated groups	1023:1056	16S rRNA gene sequencing showed that administration of B. bifidum shifted composition of the gut microbiome in mice with DSS-induced colitis in both treated and pretreated groups.
33686049	5	77	from	colitis	1012:1018	arg1	groups					1051:1056	both treated and pretreated groups	1023:1056	both treated and pretreated groups	1023:1056	16S rRNA gene sequencing showed that administration of B. bifidum shifted composition of the gut microbiome in mice with DSS-induced colitis in both treated and pretreated groups.
33686049	8	78	theme	bifidum-treated	1478:1492	arg1	group					1494:1498	the B. bifidum-treated group	1471:1498	the B. bifidum-treated group	1471:1498	On the basis of linear discriminant analysis of effect size, the abundance of the genus Escherichia-Shigella, belonging to the family Enterobacteriaceae, was reduced in the B. bifidum-treated group, indicating that pathogens were inhibited by the B. bifidum treatment.
33686049	6	79	from	increase	1138:1145	arg1	diversity					1150:1158	diversity	1150:1158	diversity of the gut microbiome	1150:1180	Mice pretreated with B. bifidum ATCC 29521 for 21 days exhibited a significant increase in diversity of the gut microbiome.
33686049	4	80	theme	mice	740:743	arg1	Treatment					710:718	Treatment	710:718	Treatment	710:718	RESULTS Treatment and pretreatment of mice with B. bifidum ATCC 29521 significantly alleviated the severity of acute colitis on the basis of clinical and pathologic indicators.
33686049	4	80	theme	mice	740:743	arg1	pretreatment					724:735	pretreatment	724:735	pretreatment	724:735	RESULTS Treatment and pretreatment of mice with B. bifidum ATCC 29521 significantly alleviated the severity of acute colitis on the basis of clinical and pathologic indicators.
33686049	3	81	dep	METHODS	480:486	arg1	study					492:496	Our study	488:496	METHODS Our study	480:496	MATERIAL AND METHODS Our study investigated therapeutic and preventive effects of B. bifidum ATCC 29521 on C57BL/6 mice with dextran sulfate sodium (DSS)-induced acute colitis via 16S ribosomal ribonucleic acid (rRNA) gene sequencing.
33686049	2	82	theme	treated	419:425	arg1	patients					446:453	treated and pretreated IBD patients	419:453	treated and pretreated IBD patients	419:453	However, information on the influence of B. bifidum on gut microbial diversity of treated and pretreated IBD patients is limited.
33686049	8	83	theme	effect	1350:1355	arg1	size					1357:1360	effect size	1350:1360	effect size	1350:1360	On the basis of linear discriminant analysis of effect size, the abundance of the genus Escherichia-Shigella, belonging to the family Enterobacteriaceae, was reduced in the B. bifidum-treated group, indicating that pathogens were inhibited by the B. bifidum treatment.
33686049	2	84	theme	gut	392:394	arg1	diversity					406:414	gut microbial diversity	392:414	gut microbial diversity	392:414	However, information on the influence of B. bifidum on gut microbial diversity of treated and pretreated IBD patients is limited.
33686049	7	85	theme	different	1264:1272	arg1	treatments					1274:1283	different treatments	1264:1283	different treatments	1264:1283	Principal coordinate analysis showed that gut microbiota structure was shaped by different treatments and time points.
33686049	2	86	theme	patients	446:453	arg1	influence					365:373	the influence	361:373	the influence of B. bifidum on gut microbial diversity of treated and pretreated IBD patients	361:453	However, information on the influence of B. bifidum on gut microbial diversity of treated and pretreated IBD patients is limited.
33686049	8	87	theme	B.	1549:1550	arg1	treatment					1560:1568	the B. bifidum treatment	1545:1568	the B. bifidum treatment	1545:1568	On the basis of linear discriminant analysis of effect size, the abundance of the genus Escherichia-Shigella, belonging to the family Enterobacteriaceae, was reduced in the B. bifidum-treated group, indicating that pathogens were inhibited by the B. bifidum treatment.
33686049	10	88	theme	genera	1859:1864	arg1	Intestinimonas					1866:1879	the genera Intestinimonas	1855:1879	the genera Intestinimonas	1855:1879	CONCLUSIONS 16S rRNA gene sequencing showed that pretreatment with B. bifidum ATCC 29521 reduced intestinal inflammation and altered the gut microbiota to favor the genera Intestinimonas and Bacteroides.
33686049	0	89	theme	Mouse	98:102	arg1	Model					104:108	a Mouse Model	96:108	a Mouse Model of Acute Colitis Induced by Dextran Sulfate Sodium	96:159	Effects of Pretreatment with Bifidobacterium bifidum Using 16S Ribosomal RNA Gene Sequencing in a Mouse Model of Acute Colitis Induced by Dextran Sulfate Sodium.
33686049	0	90	theme	16S	59:61	arg1	Sequencing					82:91	16S Ribosomal RNA Gene Sequencing	59:91	16S Ribosomal RNA Gene Sequencing	59:91	Effects of Pretreatment with Bifidobacterium bifidum Using 16S Ribosomal RNA Gene Sequencing in a Mouse Model of Acute Colitis Induced by Dextran Sulfate Sodium.
33686049	1	91	theme	ulcerative	297:306	arg1	colitis					308:314	ulcerative colitis	297:314	ulcerative colitis	297:314	BACKGROUND Bifidobacterium is a potentially effective and safe treatment for patients with inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease.
33686049	2	92	theme	pretreated	431:440	arg1	patients					446:453	treated and pretreated IBD patients	419:453	treated and pretreated IBD patients	419:453	However, information on the influence of B. bifidum on gut microbial diversity of treated and pretreated IBD patients is limited.
33686049	8	93	theme	linear	1318:1323	arg1	analysis					1338:1345	linear discriminant analysis	1318:1345	linear discriminant analysis of effect size	1318:1360	On the basis of linear discriminant analysis of effect size, the abundance of the genus Escherichia-Shigella, belonging to the family Enterobacteriaceae, was reduced in the B. bifidum-treated group, indicating that pathogens were inhibited by the B. bifidum treatment.
33686049	3	94	theme	acute	629:633	arg1	colitis					635:641	dextran sulfate sodium (DSS)-induced acute colitis	592:641	dextran sulfate sodium (DSS)-induced acute colitis	592:641	MATERIAL AND METHODS Our study investigated therapeutic and preventive effects of B. bifidum ATCC 29521 on C57BL/6 mice with dextran sulfate sodium (DSS)-induced acute colitis via 16S ribosomal ribonucleic acid (rRNA) gene sequencing.
33686049	4	95	theme	B.	750:751	arg1	ATCC					761:764	B. bifidum ATCC 29521	750:770	B. bifidum ATCC 29521	750:770	RESULTS Treatment and pretreatment of mice with B. bifidum ATCC 29521 significantly alleviated the severity of acute colitis on the basis of clinical and pathologic indicators.
33686049	5	96	theme	gut	972:974	arg1	microbiome					976:985	the gut microbiome	968:985	the gut microbiome	968:985	16S rRNA gene sequencing showed that administration of B. bifidum shifted composition of the gut microbiome in mice with DSS-induced colitis in both treated and pretreated groups.
33686049	1	97	with	patients	239:246	arg1	IBD					281:283	IBD	281:283	IBD	281:283	BACKGROUND Bifidobacterium is a potentially effective and safe treatment for patients with inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease.
33686049	1	97	with	patients	239:246	arg1	disease					272:278	inflammatory bowel disease	253:278	inflammatory bowel disease (IBD)	253:284	BACKGROUND Bifidobacterium is a potentially effective and safe treatment for patients with inflammatory bowel disease (IBD), including ulcerative colitis and Crohn's disease.
33686049	7	98	theme	gut	1225:1227	arg1	structure					1240:1248	gut microbiota structure	1225:1248	gut microbiota structure	1225:1248	Principal coordinate analysis showed that gut microbiota structure was shaped by different treatments and time points.
33686049	3	99	theme	acid	673:676	arg1	sequencing					690:699	ribosomal ribonucleic acid (rRNA) gene sequencing	651:699	ribosomal ribonucleic acid (rRNA) gene sequencing	651:699	MATERIAL AND METHODS Our study investigated therapeutic and preventive effects of B. bifidum ATCC 29521 on C57BL/6 mice with dextran sulfate sodium (DSS)-induced acute colitis via 16S ribosomal ribonucleic acid (rRNA) gene sequencing.
33686049	8	100	theme	Escherichia-Shigella	1390:1409	arg1	abundance					1367:1375	the abundance	1363:1375	the abundance of the genus Escherichia-Shigella, belonging to the family Enterobacteriaceae,	1363:1454	On the basis of linear discriminant analysis of effect size, the abundance of the genus Escherichia-Shigella, belonging to the family Enterobacteriaceae, was reduced in the B. bifidum-treated group, indicating that pathogens were inhibited by the B. bifidum treatment.
31923508	6	0	theme	stable	983:988	arg1	performance					1005:1015	a stable photocatalysis performance	981:1015	a stable photocatalysis performance	981:1015	The Cr2S3-Co3O4/PEGCS nanocomposites can maintain a stable photocatalysis performance after five cycles.
31923508	8	1	from	properties	1164:1173	arg1	research					1219:1226	this research	1214:1226	this research	1214:1226	The antibacterial test demonstrated the remarkable properties of Cr2S3-Co3O4/PEGCS nanocomposites in this research.
31923508	2	2	theme	Rhodamine	512:520	arg1	RhB					525:527	RhB	525:527	RhB	525:527	The as-prepared Cr2S3-Co3O4/PEGCS composites represented the photo-decompose efficiency against the decomposition of basic dye (Rhodamine B (RhB)).
31923508	2	2	theme	Rhodamine	512:520	arg1	B					522:522	Rhodamine B	512:522	Rhodamine B (RhB)	512:528	The as-prepared Cr2S3-Co3O4/PEGCS composites represented the photo-decompose efficiency against the decomposition of basic dye (Rhodamine B (RhB)).
31923508	0	3	from	Cr2S3-Co3O4	0:10	arg1	glycol-chitosan					28:42	polyethylene glycol-chitosan	15:42	polyethylene glycol-chitosan	15:42	Cr2S3-Co3O4 on polyethylene glycol-chitosan nanocomposites with enhanced ultraviolet light photocatalysis activity, antibacterial and antioxidant studies.
31923508	2	4	dep	dye	507:509	arg1	RhB					525:527	RhB	525:527	RhB	525:527	The as-prepared Cr2S3-Co3O4/PEGCS composites represented the photo-decompose efficiency against the decomposition of basic dye (Rhodamine B (RhB)).
31923508	2	4	dep	dye	507:509	arg1	B					522:522	Rhodamine B	512:522	Rhodamine B (RhB)	512:528	The as-prepared Cr2S3-Co3O4/PEGCS composites represented the photo-decompose efficiency against the decomposition of basic dye (Rhodamine B (RhB)).
31923508	3	5	theme	nano-catalyst	548:560	arg1	gap					541:543	The band gap	532:543	The band gap of nano-catalyst	532:560	The band gap of nano-catalyst was determined to be in the range of 2.61 to 3.32 eV.
31923508	5	6	theme	O2-	858:860	arg1	radicals					862:869	O2- radicals	858:869	O2- radicals	858:869	Under the light irradiation, the active species OH and O2- radicals were important active agents in the photocatalysis process.
31923508	5	7	from	agents	893:898	arg1	process					922:928	the photocatalysis process	903:928	the photocatalysis process	903:928	Under the light irradiation, the active species OH and O2- radicals were important active agents in the photocatalysis process.
31923508	3	8	theme	band	536:539	arg1	gap					541:543	The band gap	532:543	The band gap of nano-catalyst	532:560	The band gap of nano-catalyst was determined to be in the range of 2.61 to 3.32 eV.
31923508	1	9	theme	decorated	212:220	arg1	CS					261:262	CS	261:262	CS	261:262	A novel chromium sulfide-cobalt oxide nanostructures and decorated on Polyethylene Glycol (PEG)-chitosan (CS) nanocomposites as catalyst was synthesized by a facile method, and characterized by XRD, SEM, UV-Vis and XPS spectrum.
31923508	1	9	theme	decorated	212:220	arg1	-chitosan					250:258	decorated on Polyethylene Glycol (PEG)-chitosan	212:258	decorated on Polyethylene Glycol (PEG)-chitosan (CS)	212:263	A novel chromium sulfide-cobalt oxide nanostructures and decorated on Polyethylene Glycol (PEG)-chitosan (CS) nanocomposites as catalyst was synthesized by a facile method, and characterized by XRD, SEM, UV-Vis and XPS spectrum.
31923508	0	10	theme	antioxidant	134:144	arg1	studies					146:152	antioxidant studies	134:152	antioxidant studies	134:152	Cr2S3-Co3O4 on polyethylene glycol-chitosan nanocomposites with enhanced ultraviolet light photocatalysis activity, antibacterial and antioxidant studies.
31923508	1	11	theme	XPS	370:372	arg1	spectrum					374:381	XPS spectrum	370:381	XPS spectrum	370:381	A novel chromium sulfide-cobalt oxide nanostructures and decorated on Polyethylene Glycol (PEG)-chitosan (CS) nanocomposites as catalyst was synthesized by a facile method, and characterized by XRD, SEM, UV-Vis and XPS spectrum.
31923508	4	12	theme	highest	764:770	arg1	performance					790:800	the highest photo-degradation performance	760:800	the highest photo-degradation performance	760:800	The introduction of Cr2S3 into Co3O4 increased the photocatalytic performance slightly, and decoration of Cr2S3-Co3O4 on PEGCS, which indicated the highest photo-degradation performance.
31923508	2	13	theme	as-prepared	388:398	arg1	composites					418:427	The as-prepared Cr2S3-Co3O4/PEGCS composites	384:427	The as-prepared Cr2S3-Co3O4/PEGCS composites	384:427	The as-prepared Cr2S3-Co3O4/PEGCS composites represented the photo-decompose efficiency against the decomposition of basic dye (Rhodamine B (RhB)).
31923508	5	14	theme	light	813:817	arg1	irradiation					819:829	the light irradiation	809:829	the light irradiation	809:829	Under the light irradiation, the active species OH and O2- radicals were important active agents in the photocatalysis process.
31923508	8	15	theme	nanocomposites	1196:1209	arg1	properties					1164:1173	the remarkable properties	1149:1173	the remarkable properties of Cr2S3-Co3O4/PEGCS nanocomposites in this research	1149:1226	The antibacterial test demonstrated the remarkable properties of Cr2S3-Co3O4/PEGCS nanocomposites in this research.
31923508	8	16	theme	Cr2S3-Co3O4/PEGCS	1178:1194	arg1	nanocomposites					1196:1209	Cr2S3-Co3O4/PEGCS nanocomposites	1178:1209	Cr2S3-Co3O4/PEGCS nanocomposites	1178:1209	The antibacterial test demonstrated the remarkable properties of Cr2S3-Co3O4/PEGCS nanocomposites in this research.
31923508	1	17	theme	Polyethylene	225:236	arg1	PEG					246:248	PEG	246:248	PEG	246:248	A novel chromium sulfide-cobalt oxide nanostructures and decorated on Polyethylene Glycol (PEG)-chitosan (CS) nanocomposites as catalyst was synthesized by a facile method, and characterized by XRD, SEM, UV-Vis and XPS spectrum.
31923508	1	17	theme	Polyethylene	225:236	arg1	Glycol					238:243	Polyethylene Glycol	225:243	Polyethylene Glycol (PEG)	225:249	A novel chromium sulfide-cobalt oxide nanostructures and decorated on Polyethylene Glycol (PEG)-chitosan (CS) nanocomposites as catalyst was synthesized by a facile method, and characterized by XRD, SEM, UV-Vis and XPS spectrum.
31923508	5	18	theme	important	876:884	arg1	agents					893:898	important active agents	876:898	important active agents	876:898	Under the light irradiation, the active species OH and O2- radicals were important active agents in the photocatalysis process.
31923508	5	18	theme	important	876:884	arg1	species					843:849	the active species	832:849	the active species OH and O2- radicals	832:869	Under the light irradiation, the active species OH and O2- radicals were important active agents in the photocatalysis process.
31923508	6	19	theme	photocatalysis	990:1003	arg1	performance					1005:1015	a stable photocatalysis performance	981:1015	a stable photocatalysis performance	981:1015	The Cr2S3-Co3O4/PEGCS nanocomposites can maintain a stable photocatalysis performance after five cycles.
31923508	1	20	dep	sulfide-cobalt	172:185	arg1	nanostructures					193:206	oxide nanostructures	187:206	oxide nanostructures	187:206	A novel chromium sulfide-cobalt oxide nanostructures and decorated on Polyethylene Glycol (PEG)-chitosan (CS) nanocomposites as catalyst was synthesized by a facile method, and characterized by XRD, SEM, UV-Vis and XPS spectrum.
31923508	2	21	theme	photo-decompose	445:459	arg1	efficiency					461:470	the photo-decompose efficiency	441:470	the photo-decompose efficiency against the decomposition of basic dye (Rhodamine B (RhB))	441:529	The as-prepared Cr2S3-Co3O4/PEGCS composites represented the photo-decompose efficiency against the decomposition of basic dye (Rhodamine B (RhB)).
31923508	0	22	theme	polyethylene	15:26	arg1	glycol-chitosan					28:42	polyethylene glycol-chitosan	15:42	polyethylene glycol-chitosan	15:42	Cr2S3-Co3O4 on polyethylene glycol-chitosan nanocomposites with enhanced ultraviolet light photocatalysis activity, antibacterial and antioxidant studies.
31923508	1	23	theme	facile	313:318	arg1	method					320:325	a facile method	311:325	a facile method	311:325	A novel chromium sulfide-cobalt oxide nanostructures and decorated on Polyethylene Glycol (PEG)-chitosan (CS) nanocomposites as catalyst was synthesized by a facile method, and characterized by XRD, SEM, UV-Vis and XPS spectrum.
31923508	4	24	theme	photo-degradation	772:788	arg1	performance					790:800	the highest photo-degradation performance	760:800	the highest photo-degradation performance	760:800	The introduction of Cr2S3 into Co3O4 increased the photocatalytic performance slightly, and decoration of Cr2S3-Co3O4 on PEGCS, which indicated the highest photo-degradation performance.
31923508	7	25	theme	RhB	1092:1094	arg1	photo-degradation					1071:1087	photo-degradation	1071:1087	photo-degradation of RhB	1071:1094	Finally, the reaction mechanism of photo-degradation of RhB was put forward.
31923508	4	26	theme	Cr2S3	636:640	arg1	introduction					620:631	The introduction	616:631	The introduction of Cr2S3 into Co3O4	616:651	The introduction of Cr2S3 into Co3O4 increased the photocatalytic performance slightly, and decoration of Cr2S3-Co3O4 on PEGCS, which indicated the highest photo-degradation performance.
31923508	3	27	dep	3.32 eV	607:613	arg1	to					604:605	to	604:605	to	604:605	The band gap of nano-catalyst was determined to be in the range of 2.61 to 3.32 eV.
31923508	2	28	theme	dye	507:509	arg1	decomposition					484:496	the decomposition	480:496	the decomposition of basic dye (Rhodamine B (RhB))	480:529	The as-prepared Cr2S3-Co3O4/PEGCS composites represented the photo-decompose efficiency against the decomposition of basic dye (Rhodamine B (RhB)).
31923508	8	29	theme	antibacterial	1117:1129	arg1	test					1131:1134	The antibacterial test	1113:1134	The antibacterial test	1113:1134	The antibacterial test demonstrated the remarkable properties of Cr2S3-Co3O4/PEGCS nanocomposites in this research.
31923508	0	30	theme	ultraviolet	73:83	arg1	light					85:89	ultraviolet light	73:89	enhanced ultraviolet light photocatalysis activity	64:113	Cr2S3-Co3O4 on polyethylene glycol-chitosan nanocomposites with enhanced ultraviolet light photocatalysis activity, antibacterial and antioxidant studies.
31923508	7	31	theme	photo-degradation	1071:1087	arg1	mechanism					1058:1066	the reaction mechanism	1045:1066	the reaction mechanism of photo-degradation of RhB	1045:1094	Finally, the reaction mechanism of photo-degradation of RhB was put forward.
31923508	0	32	theme	light	85:89	arg1	activity					106:113	enhanced ultraviolet light photocatalysis activity	64:113	enhanced ultraviolet light photocatalysis activity	64:113	Cr2S3-Co3O4 on polyethylene glycol-chitosan nanocomposites with enhanced ultraviolet light photocatalysis activity, antibacterial and antioxidant studies.
31923508	7	33	theme	reaction	1049:1056	arg1	mechanism					1058:1066	the reaction mechanism	1045:1066	the reaction mechanism of photo-degradation of RhB	1045:1094	Finally, the reaction mechanism of photo-degradation of RhB was put forward.
31923508	5	34	theme	photocatalysis	907:920	arg1	process					922:928	the photocatalysis process	903:928	the photocatalysis process	903:928	Under the light irradiation, the active species OH and O2- radicals were important active agents in the photocatalysis process.
31923508	0	35	theme	enhanced	64:71	arg1	activity					106:113	enhanced ultraviolet light photocatalysis activity	64:113	enhanced ultraviolet light photocatalysis activity	64:113	Cr2S3-Co3O4 on polyethylene glycol-chitosan nanocomposites with enhanced ultraviolet light photocatalysis activity, antibacterial and antioxidant studies.
31923508	2	36	theme	Cr2S3-Co3O4/PEGCS	400:416	arg1	composites					418:427	The as-prepared Cr2S3-Co3O4/PEGCS composites	384:427	The as-prepared Cr2S3-Co3O4/PEGCS composites	384:427	The as-prepared Cr2S3-Co3O4/PEGCS composites represented the photo-decompose efficiency against the decomposition of basic dye (Rhodamine B (RhB)).
31923508	6	37	theme	Cr2S3-Co3O4/PEGCS	935:951	arg1	nanocomposites					953:966	The Cr2S3-Co3O4/PEGCS nanocomposites	931:966	The Cr2S3-Co3O4/PEGCS nanocomposites	931:966	The Cr2S3-Co3O4/PEGCS nanocomposites can maintain a stable photocatalysis performance after five cycles.
31923508	3	38	theme	3.32 eV	607:613	arg1	range					590:594	the range	586:594	the range of 2.61 to 3.32 eV	586:613	The band gap of nano-catalyst was determined to be in the range of 2.61 to 3.32 eV.
31923508	4	39	theme	photocatalytic	667:680	arg1	performance					682:692	the photocatalytic performance	663:692	the photocatalytic performance	663:692	The introduction of Cr2S3 into Co3O4 increased the photocatalytic performance slightly, and decoration of Cr2S3-Co3O4 on PEGCS, which indicated the highest photo-degradation performance.
31923508	1	40	theme	novel	157:161	arg1	sulfide-cobalt					172:185	A novel chromium sulfide-cobalt oxide nanostructures and decorated on Polyethylene Glycol (PEG)-chitosan (CS)	155:263	sulfide-cobalt	172:185	A novel chromium sulfide-cobalt oxide nanostructures and decorated on Polyethylene Glycol (PEG)-chitosan (CS) nanocomposites as catalyst was synthesized by a facile method, and characterized by XRD, SEM, UV-Vis and XPS spectrum.
31923508	8	41	theme	remarkable	1153:1162	arg1	properties					1164:1173	the remarkable properties	1149:1173	the remarkable properties of Cr2S3-Co3O4/PEGCS nanocomposites in this research	1149:1226	The antibacterial test demonstrated the remarkable properties of Cr2S3-Co3O4/PEGCS nanocomposites in this research.
31923508	1	42	theme	chromium	163:170	arg1	sulfide-cobalt					172:185	A novel chromium sulfide-cobalt oxide nanostructures and decorated on Polyethylene Glycol (PEG)-chitosan (CS)	155:263	sulfide-cobalt	172:185	A novel chromium sulfide-cobalt oxide nanostructures and decorated on Polyethylene Glycol (PEG)-chitosan (CS) nanocomposites as catalyst was synthesized by a facile method, and characterized by XRD, SEM, UV-Vis and XPS spectrum.
31923508	4	43	from	decoration	708:717	arg1	PEGCS					737:741	PEGCS	737:741	PEGCS	737:741	The introduction of Cr2S3 into Co3O4 increased the photocatalytic performance slightly, and decoration of Cr2S3-Co3O4 on PEGCS, which indicated the highest photo-degradation performance.
31923508	4	44	theme	Cr2S3-Co3O4	722:732	arg1	decoration					708:717	decoration	708:717	decoration of Cr2S3-Co3O4 on PEGCS	708:741	The introduction of Cr2S3 into Co3O4 increased the photocatalytic performance slightly, and decoration of Cr2S3-Co3O4 on PEGCS, which indicated the highest photo-degradation performance.
31923508	0	45	theme	photocatalysis	91:104	arg1	activity					106:113	enhanced ultraviolet light photocatalysis activity	64:113	enhanced ultraviolet light photocatalysis activity	64:113	Cr2S3-Co3O4 on polyethylene glycol-chitosan nanocomposites with enhanced ultraviolet light photocatalysis activity, antibacterial and antioxidant studies.
31923508	5	46	theme	active	836:841	arg1	species					843:849	the active species	832:849	the active species OH and O2- radicals	832:869	Under the light irradiation, the active species OH and O2- radicals were important active agents in the photocatalysis process.
31923508	5	46	theme	active	836:841	arg1	agents					893:898	important active agents	876:898	important active agents	876:898	Under the light irradiation, the active species OH and O2- radicals were important active agents in the photocatalysis process.
31923508	5	47	theme	active	886:891	arg1	agents					893:898	important active agents	876:898	important active agents	876:898	Under the light irradiation, the active species OH and O2- radicals were important active agents in the photocatalysis process.
31923508	5	47	theme	active	886:891	arg1	species					843:849	the active species	832:849	the active species OH and O2- radicals	832:869	Under the light irradiation, the active species OH and O2- radicals were important active agents in the photocatalysis process.
31923508	2	48	theme	basic	501:505	arg1	dye					507:509	basic dye	501:509	basic dye (Rhodamine B (RhB))	501:529	The as-prepared Cr2S3-Co3O4/PEGCS composites represented the photo-decompose efficiency against the decomposition of basic dye (Rhodamine B (RhB)).
31923508	5	49	dep	species	843:849	arg1	OH					851:852	OH	851:852	OH	851:852	Under the light irradiation, the active species OH and O2- radicals were important active agents in the photocatalysis process.
31923508	5	49	dep	species	843:849	arg1	radicals					862:869	O2- radicals	858:869	O2- radicals	858:869	Under the light irradiation, the active species OH and O2- radicals were important active agents in the photocatalysis process.
31923508	1	50	theme	oxide	187:191	arg1	nanostructures					193:206	oxide nanostructures	187:206	oxide nanostructures	187:206	A novel chromium sulfide-cobalt oxide nanostructures and decorated on Polyethylene Glycol (PEG)-chitosan (CS) nanocomposites as catalyst was synthesized by a facile method, and characterized by XRD, SEM, UV-Vis and XPS spectrum.
32359993	9	0	theme	lipid	1553:1557	arg1	metabolism					1559:1568	lipid metabolism	1553:1568	lipid metabolism in bMEC	1553:1576	The present data indicate that CD44 plays a key regulatory role in lipid metabolism in bMEC.
32359993	6	1	theme	overexpression	1063:1076	arg1	group					1078:1082	the CD44 gene overexpression group	1049:1082	the CD44 gene overexpression group	1049:1082	Additionally, elaidic acid, palmitoleic acid, tridecanoic acid, and oleic acid were markedly reduced in the CD44 gene overexpression group compared with the control group.
32359993	7	2	theme	knockdown	1223:1231	arg1	group					1233:1237	the CD44 knockdown group	1214:1237	the CD44 knockdown group	1214:1237	On the contrary, cis-5,8,11,14-eicosatetraenoic acid and stearic acid were markedly increased in the CD44 knockdown group compared with the control group.
32359993	4	3	theme	triglycerides	606:618	arg1	contents					580:587	the contents	576:587	the contents of intracellular triglycerides (TG) and cholesterol (CHOL)	576:646	In the present study, the overexpression of the CD44 gene increased the contents of intracellular triglycerides (TG) and cholesterol (CHOL), whereas knockdown of the CD44 gene decreased bMEC CHOL and TG contents.
32359993	5	4	theme	chromatography	725:738	arg1	analysis					740:747	Gas chromatography analysis	721:747	Gas chromatography analysis of fatty acid composition	721:773	Gas chromatography analysis of fatty acid composition showed that the contents of α-linolenic acid, palmitic acid, and cis-8,11,14-eicosatrienoic acid were altered due to changes in the level of expression of the CD44 gene.
32359993	6	5	theme	CD44	1053:1056	arg1	group					1078:1082	the CD44 gene overexpression group	1049:1082	the CD44 gene overexpression group	1049:1082	Additionally, elaidic acid, palmitoleic acid, tridecanoic acid, and oleic acid were markedly reduced in the CD44 gene overexpression group compared with the control group.
32359993	6	6	theme	tridecanoic	991:1001	arg1	acid					1003:1006	tridecanoic acid	991:1006	tridecanoic acid	991:1006	Additionally, elaidic acid, palmitoleic acid, tridecanoic acid, and oleic acid were markedly reduced in the CD44 gene overexpression group compared with the control group.
32359993	5	7	theme	acid	758:761	arg1	composition					763:773	fatty acid composition	752:773	fatty acid composition	752:773	Gas chromatography analysis of fatty acid composition showed that the contents of α-linolenic acid, palmitic acid, and cis-8,11,14-eicosatrienoic acid were altered due to changes in the level of expression of the CD44 gene.
32359993	9	8	theme	key	1530:1532	arg1	role					1545:1548	a key regulatory role	1528:1548	a key regulatory role	1528:1548	The present data indicate that CD44 plays a key regulatory role in lipid metabolism in bMEC.
32359993	9	9	from	metabolism	1559:1568	arg1	bMEC					1573:1576	bMEC	1573:1576	bMEC	1573:1576	The present data indicate that CD44 plays a key regulatory role in lipid metabolism in bMEC.
32359993	1	10	theme	CD44	99:102	arg1	gene					104:107	The CD44 gene	95:107	The CD44 gene	95:107	The CD44 gene encodes a cell-surface glycoprotein that participates in a variety of biological processes such as cell interactions, adhesion, hematopoiesis, and tumor metastasis.
32359993	1	10	theme	CD44	99:102	arg1	glycoprotein					132:143	a cell-surface glycoprotein	117:143	a cell-surface glycoprotein that participates in a variety of biological processes such as cell interactions, adhesion, hematopoiesis, and tumor metastasis	117:271	The CD44 gene encodes a cell-surface glycoprotein that participates in a variety of biological processes such as cell interactions, adhesion, hematopoiesis, and tumor metastasis.
32359993	8	11	theme	PCR	1288:1290	arg1	array					1292:1296	And RT2 Profiler PCR array	1272:1296	array	1292:1296	And RT2 Profiler PCR array (Qiagen, CLAB24070A Frankfurt, Germany) further suggested that overexpression or knockdown of the CD44 gene altered expression levels of functional genes associated with lipid metabolism.
32359993	1	12	gly	glycoprotein	132:143	arg1	gene					104:107	The CD44 gene	95:107	The CD44 gene	95:107	The CD44 gene encodes a cell-surface glycoprotein that participates in a variety of biological processes such as cell interactions, adhesion, hematopoiesis, and tumor metastasis.
32359993	1	12	gly	glycoprotein	132:143	arg1	glycoprotein					132:143	a cell-surface glycoprotein	117:143	a cell-surface glycoprotein that participates in a variety of biological processes such as cell interactions, adhesion, hematopoiesis, and tumor metastasis	117:271	The CD44 gene encodes a cell-surface glycoprotein that participates in a variety of biological processes such as cell interactions, adhesion, hematopoiesis, and tumor metastasis.
32359993	5	13	theme	cis-8,11,14-eicosatrienoic	840:865	arg1	acid					867:870	cis-8,11,14-eicosatrienoic acid	840:870	cis-8,11,14-eicosatrienoic acid	840:870	Gas chromatography analysis of fatty acid composition showed that the contents of α-linolenic acid, palmitic acid, and cis-8,11,14-eicosatrienoic acid were altered due to changes in the level of expression of the CD44 gene.
32359993	0	14	theme	metabolism	37:46	arg1	regulation					48:57	Lipid metabolism regulation	31:57	New function of the CD44 gene: Lipid metabolism regulation in bovine mammary epithelial cells.	0:93	New function of the CD44 gene: Lipid metabolism regulation in bovine mammary epithelial cells.
32359993	5	15	theme	acid	815:818	arg1	contents					791:798	the contents	787:798	the contents of α-linolenic acid, palmitic acid, and cis-8,11,14-eicosatrienoic acid	787:870	Gas chromatography analysis of fatty acid composition showed that the contents of α-linolenic acid, palmitic acid, and cis-8,11,14-eicosatrienoic acid were altered due to changes in the level of expression of the CD44 gene.
32359993	4	16	theme	present	515:521	arg1	study					523:527	the present study	511:527	the present study	511:527	In the present study, the overexpression of the CD44 gene increased the contents of intracellular triglycerides (TG) and cholesterol (CHOL), whereas knockdown of the CD44 gene decreased bMEC CHOL and TG contents.
32359993	5	17	theme	palmitic	821:828	arg1	acid					830:833	palmitic acid	821:833	palmitic acid	821:833	Gas chromatography analysis of fatty acid composition showed that the contents of α-linolenic acid, palmitic acid, and cis-8,11,14-eicosatrienoic acid were altered due to changes in the level of expression of the CD44 gene.
32359993	2	18	from	transcriptome	290:302	arg1	bMEC					340:343	bMEC	340:343	bMEC	340:343	We compared the transcriptome in bovine mammary epithelial cells (bMEC) of Chinese Holstein dairy cows producing milk of high and low fat contents.
32359993	2	18	from	transcriptome	290:302	arg1	cells					333:337	bovine mammary epithelial cells	307:337	bovine mammary epithelial cells (bMEC)	307:344	We compared the transcriptome in bovine mammary epithelial cells (bMEC) of Chinese Holstein dairy cows producing milk of high and low fat contents.
32359993	8	19	theme	gene	1401:1404	arg1	knockdown					1379:1387	knockdown	1379:1387	knockdown	1379:1387	And RT2 Profiler PCR array (Qiagen, CLAB24070A Frankfurt, Germany) further suggested that overexpression or knockdown of the CD44 gene altered expression levels of functional genes associated with lipid metabolism.
32359993	8	19	theme	gene	1401:1404	arg1	overexpression					1361:1374	overexpression	1361:1374	overexpression	1361:1374	And RT2 Profiler PCR array (Qiagen, CLAB24070A Frankfurt, Germany) further suggested that overexpression or knockdown of the CD44 gene altered expression levels of functional genes associated with lipid metabolism.
32359993	0	20	theme	mammary	69:75	arg1	cells					88:92	bovine mammary epithelial cells	62:92	bovine mammary epithelial cells	62:92	New function of the CD44 gene: Lipid metabolism regulation in bovine mammary epithelial cells.
32359993	8	21	theme	expression	1414:1423	arg1	levels					1425:1430	expression levels	1414:1430	expression levels of functional genes associated with lipid metabolism	1414:1483	And RT2 Profiler PCR array (Qiagen, CLAB24070A Frankfurt, Germany) further suggested that overexpression or knockdown of the CD44 gene altered expression levels of functional genes associated with lipid metabolism.
32359993	7	22	theme	CD44	1218:1221	arg1	group					1233:1237	the CD44 knockdown group	1214:1237	the CD44 knockdown group	1214:1237	On the contrary, cis-5,8,11,14-eicosatetraenoic acid and stearic acid were markedly increased in the CD44 knockdown group compared with the control group.
32359993	2	23	theme	dairy	366:370	arg1	cows					372:375	Chinese Holstein dairy cows	349:375	Chinese Holstein dairy cows producing milk of high and low fat contents	349:419	We compared the transcriptome in bovine mammary epithelial cells (bMEC) of Chinese Holstein dairy cows producing milk of high and low fat contents.
32359993	2	24	theme	fat	408:410	arg1	contents					412:419	high and low fat contents	395:419	high and low fat contents	395:419	We compared the transcriptome in bovine mammary epithelial cells (bMEC) of Chinese Holstein dairy cows producing milk of high and low fat contents.
32359993	8	25	theme	RT2	1276:1278	arg1	array					1292:1296	And RT2 Profiler PCR array	1272:1296	array	1292:1296	And RT2 Profiler PCR array (Qiagen, CLAB24070A Frankfurt, Germany) further suggested that overexpression or knockdown of the CD44 gene altered expression levels of functional genes associated with lipid metabolism.
32359993	2	26	theme	Chinese	349:355	arg1	cows					372:375	Chinese Holstein dairy cows	349:375	Chinese Holstein dairy cows producing milk of high and low fat contents	349:419	We compared the transcriptome in bovine mammary epithelial cells (bMEC) of Chinese Holstein dairy cows producing milk of high and low fat contents.
32359993	0	27	theme	New	0:2	arg1	function					4:11	New function	0:11	New function of the CD44 gene: Lipid metabolism regulation in bovine mammary epithelial cells.	0:93	New function of the CD44 gene: Lipid metabolism regulation in bovine mammary epithelial cells.
32359993	8	28	theme	genes	1446:1450	arg1	levels					1425:1430	expression levels	1414:1430	expression levels of functional genes associated with lipid metabolism	1414:1483	And RT2 Profiler PCR array (Qiagen, CLAB24070A Frankfurt, Germany) further suggested that overexpression or knockdown of the CD44 gene altered expression levels of functional genes associated with lipid metabolism.
32359993	4	29	theme	CD44	674:677	arg1	gene					679:682	the CD44 gene	670:682	the CD44 gene	670:682	In the present study, the overexpression of the CD44 gene increased the contents of intracellular triglycerides (TG) and cholesterol (CHOL), whereas knockdown of the CD44 gene decreased bMEC CHOL and TG contents.
32359993	0	30	dep	function	4:11	arg1	regulation					48:57	Lipid metabolism regulation	31:57	New function of the CD44 gene: Lipid metabolism regulation in bovine mammary epithelial cells.	0:93	New function of the CD44 gene: Lipid metabolism regulation in bovine mammary epithelial cells.
32359993	9	31	theme	present	1490:1496	arg1	data					1498:1501	The present data	1486:1501	The present data	1486:1501	The present data indicate that CD44 plays a key regulatory role in lipid metabolism in bMEC.
32359993	6	32	theme	elaidic	959:965	arg1	acid					967:970	elaidic acid	959:970	elaidic acid	959:970	Additionally, elaidic acid, palmitoleic acid, tridecanoic acid, and oleic acid were markedly reduced in the CD44 gene overexpression group compared with the control group.
32359993	0	33	theme	CD44	20:23	arg1	gene					25:28	the CD44 gene	16:28	the CD44 gene	16:28	New function of the CD44 gene: Lipid metabolism regulation in bovine mammary epithelial cells.
32359993	5	34	theme	CD44	934:937	arg1	gene					939:942	the CD44 gene	930:942	the CD44 gene	930:942	Gas chromatography analysis of fatty acid composition showed that the contents of α-linolenic acid, palmitic acid, and cis-8,11,14-eicosatrienoic acid were altered due to changes in the level of expression of the CD44 gene.
32359993	2	35	theme	epithelial	322:331	arg1	bMEC					340:343	bMEC	340:343	bMEC	340:343	We compared the transcriptome in bovine mammary epithelial cells (bMEC) of Chinese Holstein dairy cows producing milk of high and low fat contents.
32359993	2	35	theme	epithelial	322:331	arg1	cells					333:337	bovine mammary epithelial cells	307:337	bovine mammary epithelial cells (bMEC)	307:344	We compared the transcriptome in bovine mammary epithelial cells (bMEC) of Chinese Holstein dairy cows producing milk of high and low fat contents.
32359993	4	36	theme	TG	708:709	arg1	contents					711:718	bMEC CHOL and TG contents	694:718	contents	711:718	In the present study, the overexpression of the CD44 gene increased the contents of intracellular triglycerides (TG) and cholesterol (CHOL), whereas knockdown of the CD44 gene decreased bMEC CHOL and TG contents.
32359993	2	37	theme	bovine	307:312	arg1	bMEC					340:343	bMEC	340:343	bMEC	340:343	We compared the transcriptome in bovine mammary epithelial cells (bMEC) of Chinese Holstein dairy cows producing milk of high and low fat contents.
32359993	2	37	theme	bovine	307:312	arg1	cells					333:337	bovine mammary epithelial cells	307:337	bovine mammary epithelial cells (bMEC)	307:344	We compared the transcriptome in bovine mammary epithelial cells (bMEC) of Chinese Holstein dairy cows producing milk of high and low fat contents.
32359993	3	38	theme	milk	488:491	arg1	synthesis					497:505	milk fat synthesis	488:505	milk fat synthesis	488:505	Our results suggest that CD44 might be a candidate gene affecting milk fat synthesis.
32359993	6	39	theme	oleic	1013:1017	arg1	acid					1019:1022	oleic acid	1013:1022	oleic acid	1013:1022	Additionally, elaidic acid, palmitoleic acid, tridecanoic acid, and oleic acid were markedly reduced in the CD44 gene overexpression group compared with the control group.
32359993	3	40	theme	candidate	463:471	arg1	CD44					447:450	CD44	447:450	CD44	447:450	Our results suggest that CD44 might be a candidate gene affecting milk fat synthesis.
32359993	3	40	theme	candidate	463:471	arg1	gene					473:476	a candidate gene	461:476	a candidate gene affecting milk fat synthesis	461:505	Our results suggest that CD44 might be a candidate gene affecting milk fat synthesis.
32359993	4	41	theme	intracellular	592:604	arg1	triglycerides					606:618	intracellular triglycerides	592:618	intracellular triglycerides (TG)	592:623	In the present study, the overexpression of the CD44 gene increased the contents of intracellular triglycerides (TG) and cholesterol (CHOL), whereas knockdown of the CD44 gene decreased bMEC CHOL and TG contents.
32359993	4	41	theme	intracellular	592:604	arg1	TG					621:622	TG	621:622	TG	621:622	In the present study, the overexpression of the CD44 gene increased the contents of intracellular triglycerides (TG) and cholesterol (CHOL), whereas knockdown of the CD44 gene decreased bMEC CHOL and TG contents.
32359993	9	42	theme	regulatory	1534:1543	arg1	role					1545:1548	a key regulatory role	1528:1548	a key regulatory role	1528:1548	The present data indicate that CD44 plays a key regulatory role in lipid metabolism in bMEC.
32359993	4	43	theme	CD44	556:559	arg1	gene					561:564	the CD44 gene	552:564	the CD44 gene	552:564	In the present study, the overexpression of the CD44 gene increased the contents of intracellular triglycerides (TG) and cholesterol (CHOL), whereas knockdown of the CD44 gene decreased bMEC CHOL and TG contents.
32359993	7	44	theme	cis-5,8,11,14-eicosatetraenoic	1134:1163	arg1	acid					1165:1168	cis-5,8,11,14-eicosatetraenoic acid	1134:1168	cis-5,8,11,14-eicosatetraenoic acid	1134:1168	On the contrary, cis-5,8,11,14-eicosatetraenoic acid and stearic acid were markedly increased in the CD44 knockdown group compared with the control group.
32359993	3	45	theme	fat	493:495	arg1	synthesis					497:505	milk fat synthesis	488:505	milk fat synthesis	488:505	Our results suggest that CD44 might be a candidate gene affecting milk fat synthesis.
32359993	5	46	from	changes	892:898	arg1	level					907:911	the level	903:911	the level of expression of the CD44 gene	903:942	Gas chromatography analysis of fatty acid composition showed that the contents of α-linolenic acid, palmitic acid, and cis-8,11,14-eicosatrienoic acid were altered due to changes in the level of expression of the CD44 gene.
32359993	6	47	theme	gene	1058:1061	arg1	group					1078:1082	the CD44 gene overexpression group	1049:1082	the CD44 gene overexpression group	1049:1082	Additionally, elaidic acid, palmitoleic acid, tridecanoic acid, and oleic acid were markedly reduced in the CD44 gene overexpression group compared with the control group.
32359993	6	48	dep	reduced	1038:1044	arg1	compared					1084:1091	compared	1084:1091	compared with the control group	1084:1114	Additionally, elaidic acid, palmitoleic acid, tridecanoic acid, and oleic acid were markedly reduced in the CD44 gene overexpression group compared with the control group.
32359993	5	49	theme	fatty	752:756	arg1	composition					763:773	fatty acid composition	752:773	fatty acid composition	752:773	Gas chromatography analysis of fatty acid composition showed that the contents of α-linolenic acid, palmitic acid, and cis-8,11,14-eicosatrienoic acid were altered due to changes in the level of expression of the CD44 gene.
32359993	8	50	dep	array	1292:1296	arg1	Qiagen					1299:1304	Qiagen	1299:1304	Qiagen	1299:1304	And RT2 Profiler PCR array (Qiagen, CLAB24070A Frankfurt, Germany) further suggested that overexpression or knockdown of the CD44 gene altered expression levels of functional genes associated with lipid metabolism.
32359993	8	50	dep	array	1292:1296	arg1	Frankfurt					1318:1326	CLAB24070A Frankfurt	1307:1326	CLAB24070A Frankfurt	1307:1326	And RT2 Profiler PCR array (Qiagen, CLAB24070A Frankfurt, Germany) further suggested that overexpression or knockdown of the CD44 gene altered expression levels of functional genes associated with lipid metabolism.
32359993	8	50	dep	array	1292:1296	arg1	Germany					1329:1335	Germany	1329:1335	Germany	1329:1335	And RT2 Profiler PCR array (Qiagen, CLAB24070A Frankfurt, Germany) further suggested that overexpression or knockdown of the CD44 gene altered expression levels of functional genes associated with lipid metabolism.
32359993	5	51	theme	composition	763:773	arg1	analysis					740:747	Gas chromatography analysis	721:747	Gas chromatography analysis of fatty acid composition	721:773	Gas chromatography analysis of fatty acid composition showed that the contents of α-linolenic acid, palmitic acid, and cis-8,11,14-eicosatrienoic acid were altered due to changes in the level of expression of the CD44 gene.
32359993	0	52	from	regulation	48:57	arg1	cells					88:92	bovine mammary epithelial cells	62:92	bovine mammary epithelial cells	62:92	New function of the CD44 gene: Lipid metabolism regulation in bovine mammary epithelial cells.
32359993	5	53	theme	acid	830:833	arg1	contents					791:798	the contents	787:798	the contents of α-linolenic acid, palmitic acid, and cis-8,11,14-eicosatrienoic acid	787:870	Gas chromatography analysis of fatty acid composition showed that the contents of α-linolenic acid, palmitic acid, and cis-8,11,14-eicosatrienoic acid were altered due to changes in the level of expression of the CD44 gene.
32359993	6	54	theme	control	1102:1108	arg1	group					1110:1114	the control group	1098:1114	the control group	1098:1114	Additionally, elaidic acid, palmitoleic acid, tridecanoic acid, and oleic acid were markedly reduced in the CD44 gene overexpression group compared with the control group.
32359993	5	55	theme	α-linolenic	803:813	arg1	acid					815:818	α-linolenic acid	803:818	α-linolenic acid	803:818	Gas chromatography analysis of fatty acid composition showed that the contents of α-linolenic acid, palmitic acid, and cis-8,11,14-eicosatrienoic acid were altered due to changes in the level of expression of the CD44 gene.
32359993	8	56	theme	CD44	1396:1399	arg1	gene					1401:1404	the CD44 gene	1392:1404	the CD44 gene	1392:1404	And RT2 Profiler PCR array (Qiagen, CLAB24070A Frankfurt, Germany) further suggested that overexpression or knockdown of the CD44 gene altered expression levels of functional genes associated with lipid metabolism.
32359993	7	57	theme	control	1257:1263	arg1	group					1265:1269	the control group	1253:1269	the control group	1253:1269	On the contrary, cis-5,8,11,14-eicosatetraenoic acid and stearic acid were markedly increased in the CD44 knockdown group compared with the control group.
32359993	0	58	theme	bovine	62:67	arg1	cells					88:92	bovine mammary epithelial cells	62:92	bovine mammary epithelial cells	62:92	New function of the CD44 gene: Lipid metabolism regulation in bovine mammary epithelial cells.
32359993	4	59	theme	cholesterol	629:639	arg1	contents					580:587	the contents	576:587	the contents of intracellular triglycerides (TG) and cholesterol (CHOL)	576:646	In the present study, the overexpression of the CD44 gene increased the contents of intracellular triglycerides (TG) and cholesterol (CHOL), whereas knockdown of the CD44 gene decreased bMEC CHOL and TG contents.
32359993	0	60	theme	epithelial	77:86	arg1	cells					88:92	bovine mammary epithelial cells	62:92	bovine mammary epithelial cells	62:92	New function of the CD44 gene: Lipid metabolism regulation in bovine mammary epithelial cells.
32359993	4	61	theme	gene	679:682	arg1	knockdown					657:665	knockdown	657:665	knockdown of the CD44 gene	657:682	In the present study, the overexpression of the CD44 gene increased the contents of intracellular triglycerides (TG) and cholesterol (CHOL), whereas knockdown of the CD44 gene decreased bMEC CHOL and TG contents.
32359993	1	62	theme	biological	179:188	arg1	interactions					213:224	cell interactions	208:224	cell interactions	208:224	The CD44 gene encodes a cell-surface glycoprotein that participates in a variety of biological processes such as cell interactions, adhesion, hematopoiesis, and tumor metastasis.
32359993	1	62	theme	biological	179:188	arg1	processes					190:198	biological processes	179:198	biological processes such as cell interactions, adhesion, hematopoiesis, and tumor metastasis	179:271	The CD44 gene encodes a cell-surface glycoprotein that participates in a variety of biological processes such as cell interactions, adhesion, hematopoiesis, and tumor metastasis.
32359993	8	63	theme	functional	1435:1444	arg1	genes					1446:1450	functional genes	1435:1450	functional genes associated with lipid metabolism	1435:1483	And RT2 Profiler PCR array (Qiagen, CLAB24070A Frankfurt, Germany) further suggested that overexpression or knockdown of the CD44 gene altered expression levels of functional genes associated with lipid metabolism.
32359993	4	64	theme	bMEC	694:697	arg1	CHOL					699:702	bMEC CHOL and TG contents	694:718	CHOL	699:702	In the present study, the overexpression of the CD44 gene increased the contents of intracellular triglycerides (TG) and cholesterol (CHOL), whereas knockdown of the CD44 gene decreased bMEC CHOL and TG contents.
32359993	2	65	theme	Holstein	357:364	arg1	cows					372:375	Chinese Holstein dairy cows	349:375	Chinese Holstein dairy cows producing milk of high and low fat contents	349:419	We compared the transcriptome in bovine mammary epithelial cells (bMEC) of Chinese Holstein dairy cows producing milk of high and low fat contents.
32359993	5	66	theme	Gas	721:723	arg1	analysis					740:747	Gas chromatography analysis	721:747	Gas chromatography analysis of fatty acid composition	721:773	Gas chromatography analysis of fatty acid composition showed that the contents of α-linolenic acid, palmitic acid, and cis-8,11,14-eicosatrienoic acid were altered due to changes in the level of expression of the CD44 gene.
32359993	5	67	theme	acid	867:870	arg1	contents					791:798	the contents	787:798	the contents of α-linolenic acid, palmitic acid, and cis-8,11,14-eicosatrienoic acid	787:870	Gas chromatography analysis of fatty acid composition showed that the contents of α-linolenic acid, palmitic acid, and cis-8,11,14-eicosatrienoic acid were altered due to changes in the level of expression of the CD44 gene.
32359993	6	68	theme	palmitoleic	973:983	arg1	acid					985:988	palmitoleic acid	973:988	palmitoleic acid	973:988	Additionally, elaidic acid, palmitoleic acid, tridecanoic acid, and oleic acid were markedly reduced in the CD44 gene overexpression group compared with the control group.
32359993	2	69	theme	low	404:406	arg1	contents					412:419	high and low fat contents	395:419	high and low fat contents	395:419	We compared the transcriptome in bovine mammary epithelial cells (bMEC) of Chinese Holstein dairy cows producing milk of high and low fat contents.
32359993	1	70	theme	tumor	256:260	arg1	metastasis					262:271	tumor metastasis	256:271	tumor metastasis	256:271	The CD44 gene encodes a cell-surface glycoprotein that participates in a variety of biological processes such as cell interactions, adhesion, hematopoiesis, and tumor metastasis.
32359993	1	70	theme	tumor	256:260	arg1	interactions					213:224	cell interactions	208:224	cell interactions	208:224	The CD44 gene encodes a cell-surface glycoprotein that participates in a variety of biological processes such as cell interactions, adhesion, hematopoiesis, and tumor metastasis.
32359993	2	71	theme	high	395:398	arg1	contents					412:419	high and low fat contents	395:419	high and low fat contents	395:419	We compared the transcriptome in bovine mammary epithelial cells (bMEC) of Chinese Holstein dairy cows producing milk of high and low fat contents.
32359993	8	72	theme	lipid	1468:1472	arg1	metabolism					1474:1483	lipid metabolism	1468:1483	lipid metabolism	1468:1483	And RT2 Profiler PCR array (Qiagen, CLAB24070A Frankfurt, Germany) further suggested that overexpression or knockdown of the CD44 gene altered expression levels of functional genes associated with lipid metabolism.
32359993	8	73	theme	Profiler	1280:1286	arg1	array					1292:1296	And RT2 Profiler PCR array	1272:1296	array	1292:1296	And RT2 Profiler PCR array (Qiagen, CLAB24070A Frankfurt, Germany) further suggested that overexpression or knockdown of the CD44 gene altered expression levels of functional genes associated with lipid metabolism.
32359993	0	74	theme	gene	25:28	arg1	function					4:11	New function	0:11	New function of the CD44 gene: Lipid metabolism regulation in bovine mammary epithelial cells.	0:93	New function of the CD44 gene: Lipid metabolism regulation in bovine mammary epithelial cells.
32359993	1	75	theme	processes	190:198	arg1	interactions					213:224	cell interactions	208:224	cell interactions	208:224	The CD44 gene encodes a cell-surface glycoprotein that participates in a variety of biological processes such as cell interactions, adhesion, hematopoiesis, and tumor metastasis.
32359993	1	75	theme	processes	190:198	arg1	variety					168:174	a variety	166:174	a variety of biological processes such as cell interactions, adhesion, hematopoiesis, and tumor metastasis	166:271	The CD44 gene encodes a cell-surface glycoprotein that participates in a variety of biological processes such as cell interactions, adhesion, hematopoiesis, and tumor metastasis.
32359993	1	75	theme	processes	190:198	arg1	processes					190:198	biological processes	179:198	biological processes such as cell interactions, adhesion, hematopoiesis, and tumor metastasis	179:271	The CD44 gene encodes a cell-surface glycoprotein that participates in a variety of biological processes such as cell interactions, adhesion, hematopoiesis, and tumor metastasis.
32359993	2	76	theme	cows	372:375	arg1	transcriptome					290:302	the transcriptome	286:302	the transcriptome in bovine mammary epithelial cells (bMEC) of Chinese Holstein dairy cows producing milk of high and low fat contents	286:419	We compared the transcriptome in bovine mammary epithelial cells (bMEC) of Chinese Holstein dairy cows producing milk of high and low fat contents.
32359993	0	77	theme	Lipid	31:35	arg1	regulation					48:57	Lipid metabolism regulation	31:57	New function of the CD44 gene: Lipid metabolism regulation in bovine mammary epithelial cells.	0:93	New function of the CD44 gene: Lipid metabolism regulation in bovine mammary epithelial cells.
32359993	5	78	theme	gene	939:942	arg1	expression					916:925	expression	916:925	expression of the CD44 gene	916:942	Gas chromatography analysis of fatty acid composition showed that the contents of α-linolenic acid, palmitic acid, and cis-8,11,14-eicosatrienoic acid were altered due to changes in the level of expression of the CD44 gene.
32359993	2	79	theme	contents	412:419	arg1	milk					387:390	milk	387:390	milk of high and low fat contents	387:419	We compared the transcriptome in bovine mammary epithelial cells (bMEC) of Chinese Holstein dairy cows producing milk of high and low fat contents.
32359993	1	80	theme	cell-surface	119:130	arg1	gene					104:107	The CD44 gene	95:107	The CD44 gene	95:107	The CD44 gene encodes a cell-surface glycoprotein that participates in a variety of biological processes such as cell interactions, adhesion, hematopoiesis, and tumor metastasis.
32359993	1	80	theme	cell-surface	119:130	arg1	glycoprotein					132:143	a cell-surface glycoprotein	117:143	a cell-surface glycoprotein that participates in a variety of biological processes such as cell interactions, adhesion, hematopoiesis, and tumor metastasis	117:271	The CD44 gene encodes a cell-surface glycoprotein that participates in a variety of biological processes such as cell interactions, adhesion, hematopoiesis, and tumor metastasis.
32359993	2	81	theme	mammary	314:320	arg1	bMEC					340:343	bMEC	340:343	bMEC	340:343	We compared the transcriptome in bovine mammary epithelial cells (bMEC) of Chinese Holstein dairy cows producing milk of high and low fat contents.
32359993	2	81	theme	mammary	314:320	arg1	cells					333:337	bovine mammary epithelial cells	307:337	bovine mammary epithelial cells (bMEC)	307:344	We compared the transcriptome in bovine mammary epithelial cells (bMEC) of Chinese Holstein dairy cows producing milk of high and low fat contents.
32359993	7	82	dep	increased	1201:1209	arg1	compared					1239:1246	compared	1239:1246	compared with the control group	1239:1269	On the contrary, cis-5,8,11,14-eicosatetraenoic acid and stearic acid were markedly increased in the CD44 knockdown group compared with the control group.
32359993	7	83	theme	stearic	1174:1180	arg1	acid					1182:1185	stearic acid	1174:1185	stearic acid	1174:1185	On the contrary, cis-5,8,11,14-eicosatetraenoic acid and stearic acid were markedly increased in the CD44 knockdown group compared with the control group.
32359993	8	84	theme	CLAB24070A	1307:1316	arg1	Qiagen					1299:1304	Qiagen	1299:1304	Qiagen	1299:1304	And RT2 Profiler PCR array (Qiagen, CLAB24070A Frankfurt, Germany) further suggested that overexpression or knockdown of the CD44 gene altered expression levels of functional genes associated with lipid metabolism.
32359993	8	84	theme	CLAB24070A	1307:1316	arg1	Frankfurt					1318:1326	CLAB24070A Frankfurt	1307:1326	CLAB24070A Frankfurt	1307:1326	And RT2 Profiler PCR array (Qiagen, CLAB24070A Frankfurt, Germany) further suggested that overexpression or knockdown of the CD44 gene altered expression levels of functional genes associated with lipid metabolism.
32359993	1	85	theme	cell	208:211	arg1	interactions					213:224	cell interactions	208:224	cell interactions	208:224	The CD44 gene encodes a cell-surface glycoprotein that participates in a variety of biological processes such as cell interactions, adhesion, hematopoiesis, and tumor metastasis.
32359993	1	85	theme	cell	208:211	arg1	metastasis					262:271	tumor metastasis	256:271	tumor metastasis	256:271	The CD44 gene encodes a cell-surface glycoprotein that participates in a variety of biological processes such as cell interactions, adhesion, hematopoiesis, and tumor metastasis.
32359993	1	85	theme	cell	208:211	arg1	hematopoiesis					237:249	hematopoiesis	237:249	hematopoiesis	237:249	The CD44 gene encodes a cell-surface glycoprotein that participates in a variety of biological processes such as cell interactions, adhesion, hematopoiesis, and tumor metastasis.
32359993	1	85	theme	cell	208:211	arg1	adhesion					227:234	adhesion	227:234	adhesion	227:234	The CD44 gene encodes a cell-surface glycoprotein that participates in a variety of biological processes such as cell interactions, adhesion, hematopoiesis, and tumor metastasis.
32359993	5	86	theme	expression	916:925	arg1	level					907:911	the level	903:911	the level of expression of the CD44 gene	903:942	Gas chromatography analysis of fatty acid composition showed that the contents of α-linolenic acid, palmitic acid, and cis-8,11,14-eicosatrienoic acid were altered due to changes in the level of expression of the CD44 gene.
32359993	4	87	theme	gene	561:564	arg1	overexpression					534:547	the overexpression	530:547	the overexpression of the CD44 gene	530:564	In the present study, the overexpression of the CD44 gene increased the contents of intracellular triglycerides (TG) and cholesterol (CHOL), whereas knockdown of the CD44 gene decreased bMEC CHOL and TG contents.
34337038	0	0	theme	Mushroom	89:96	arg1	Residue					98:104	Mushroom Residue	89:104	Mushroom Residue	89:104	Study on Microbial Community Succession and Functional Analysis during Biodegradation of Mushroom Residue.
34337038	3	1	theme	total	672:676	arg1	bacteria					678:685	the total bacteria	668:685	the total bacteria	668:685	The results showed that Actinobacteria, Firmicutes, Proteobacteria, Bacteroidetes, and Chloroflexi were the main microorganisms in the composting process, accounting for 98.9%-99.7% of the total bacteria.
34337038	5	2	theme	stage	1173:1177	arg1	correspondence					1179:1192	significant stage correspondence	1161:1192	significant stage correspondence	1161:1192	It indicated that the number of identified proteins related to cellulose decomposition and the number of differentially expressed proteins were significantly enriched, and the functional proteins related to cellulose decomposition had significant stage correspondence.
34337038	5	3	contain	had	1157:1159	arg1	proteins					1113:1120	the functional proteins	1098:1120	the functional proteins related to cellulose decomposition	1098:1155	It indicated that the number of identified proteins related to cellulose decomposition and the number of differentially expressed proteins were significantly enriched, and the functional proteins related to cellulose decomposition had significant stage correspondence.
34337038	5	3	contain	had	1157:1159	arg2	correspondence					1179:1192	significant stage correspondence	1161:1192	significant stage correspondence	1161:1192	It indicated that the number of identified proteins related to cellulose decomposition and the number of differentially expressed proteins were significantly enriched, and the functional proteins related to cellulose decomposition had significant stage correspondence.
34337038	1	4	theme	mushroom	258:265	arg1	samples					275:281	mushroom residue samples	258:281	mushroom residue samples	258:281	In this study, 16S rRNA high-throughput sequencing technology was used to analyze the composition and diversity of bacterial and fungal communities in mushroom residue samples at different composting stages.
34337038	5	5	theme	proteins	969:976	arg1	number					1021:1026	the number	1017:1026	the number of differentially expressed proteins	1017:1063	It indicated that the number of identified proteins related to cellulose decomposition and the number of differentially expressed proteins were significantly enriched, and the functional proteins related to cellulose decomposition had significant stage correspondence.
34337038	5	5	theme	proteins	969:976	arg1	number					948:953	the number	944:953	the number of identified proteins related to cellulose decomposition	944:1011	It indicated that the number of identified proteins related to cellulose decomposition and the number of differentially expressed proteins were significantly enriched, and the functional proteins related to cellulose decomposition had significant stage correspondence.
34337038	1	6	theme	residue	267:273	arg1	samples					275:281	mushroom residue samples	258:281	mushroom residue samples	258:281	In this study, 16S rRNA high-throughput sequencing technology was used to analyze the composition and diversity of bacterial and fungal communities in mushroom residue samples at different composting stages.
34337038	0	7	theme	Residue	98:104	arg1	Biodegradation					71:84	Biodegradation	71:84	Biodegradation of Mushroom Residue	71:104	Study on Microbial Community Succession and Functional Analysis during Biodegradation of Mushroom Residue.
34337038	5	8	theme	expressed	1046:1054	arg1	proteins					1056:1063	differentially expressed proteins	1031:1063	differentially expressed proteins	1031:1063	It indicated that the number of identified proteins related to cellulose decomposition and the number of differentially expressed proteins were significantly enriched, and the functional proteins related to cellulose decomposition had significant stage correspondence.
34337038	4	9	theme	proteins	900:907	arg1	total					885:889	anda total	880:889	anda total of 22815 proteins	880:907	Furthermore, in order to obtain the protein expressed in each stage of composting, the nonstandard quantitative method (label free) was used to analyze it quantitatively by mass spectrometry, anda total of 22815 proteins were identified.
34337038	5	10	theme	proteins	1056:1063	arg1	number					1021:1026	the number	1017:1026	the number of differentially expressed proteins	1017:1063	It indicated that the number of identified proteins related to cellulose decomposition and the number of differentially expressed proteins were significantly enriched, and the functional proteins related to cellulose decomposition had significant stage correspondence.
34337038	5	10	theme	proteins	1056:1063	arg1	number					948:953	the number	944:953	the number of identified proteins related to cellulose decomposition	944:1011	It indicated that the number of identified proteins related to cellulose decomposition and the number of differentially expressed proteins were significantly enriched, and the functional proteins related to cellulose decomposition had significant stage correspondence.
34337038	3	11	from	microorganisms	596:609	arg1	process					629:635	the composting process	614:635	the composting process	614:635	The results showed that Actinobacteria, Firmicutes, Proteobacteria, Bacteroidetes, and Chloroflexi were the main microorganisms in the composting process, accounting for 98.9%-99.7% of the total bacteria.
34337038	4	12	theme	22815	894:898	arg1	proteins					900:907	22815 proteins	894:907	22815 proteins	894:907	Furthermore, in order to obtain the protein expressed in each stage of composting, the nonstandard quantitative method (label free) was used to analyze it quantitatively by mass spectrometry, anda total of 22815 proteins were identified.
34337038	3	13	theme	bacteria	678:685	arg1	%					663:663	98.9%-99.7%	653:663	98.9%-99.7% of the total bacteria	653:685	The results showed that Actinobacteria, Firmicutes, Proteobacteria, Bacteroidetes, and Chloroflexi were the main microorganisms in the composting process, accounting for 98.9%-99.7% of the total bacteria.
34337038	3	13	theme	bacteria	678:685	arg1	bacteria					678:685	the total bacteria	668:685	the total bacteria	668:685	The results showed that Actinobacteria, Firmicutes, Proteobacteria, Bacteroidetes, and Chloroflexi were the main microorganisms in the composting process, accounting for 98.9%-99.7% of the total bacteria.
34337038	4	14	theme	composting	759:768	arg1	stage					750:754	each stage	745:754	each stage of composting	745:768	Furthermore, in order to obtain the protein expressed in each stage of composting, the nonstandard quantitative method (label free) was used to analyze it quantitatively by mass spectrometry, anda total of 22815 proteins were identified.
34337038	4	15	dep	method	800:805	arg1	label					808:812	label	808:812	label free	808:817	Furthermore, in order to obtain the protein expressed in each stage of composting, the nonstandard quantitative method (label free) was used to analyze it quantitatively by mass spectrometry, anda total of 22815 proteins were identified.
34337038	2	16	theme	pile	391:394	arg1	center					377:382	the center	373:382	the center of the pile	373:394	During the composting process, the maximum temperature in the center of the pile can reach 52.4°C, and the temperature above 50°C has been maintained for about 8 days.
34337038	2	17	from	temperature	358:368	arg1	center					377:382	the center	373:382	the center of the pile	373:394	During the composting process, the maximum temperature in the center of the pile can reach 52.4°C, and the temperature above 50°C has been maintained for about 8 days.
34337038	2	18	theme	composting	326:335	arg1	process					337:343	the composting process	322:343	the composting process	322:343	During the composting process, the maximum temperature in the center of the pile can reach 52.4°C, and the temperature above 50°C has been maintained for about 8 days.
34337038	2	19	theme	above	434:438	arg1	50°C					440:443	the temperature above 50°C	418:443	the temperature above 50°C	418:443	During the composting process, the maximum temperature in the center of the pile can reach 52.4°C, and the temperature above 50°C has been maintained for about 8 days.
34337038	4	20	used	used	824:827	arg2	method					800:805	the nonstandard quantitative method	771:805	the nonstandard quantitative method (label free)	771:818	Furthermore, in order to obtain the protein expressed in each stage of composting, the nonstandard quantitative method (label free) was used to analyze it quantitatively by mass spectrometry, anda total of 22815 proteins were identified.
34337038	0	21	theme	Community	19:27	arg1	Succession					29:38	Microbial Community Succession	9:38	Microbial Community Succession	9:38	Study on Microbial Community Succession and Functional Analysis during Biodegradation of Mushroom Residue.
34337038	1	22	theme	different	286:294	arg1	stages					307:312	different composting stages	286:312	different composting stages	286:312	In this study, 16S rRNA high-throughput sequencing technology was used to analyze the composition and diversity of bacterial and fungal communities in mushroom residue samples at different composting stages.
34337038	5	23	theme	related	978:984	arg1	proteins					969:976	identified proteins	958:976	identified proteins related to cellulose decomposition	958:1011	It indicated that the number of identified proteins related to cellulose decomposition and the number of differentially expressed proteins were significantly enriched, and the functional proteins related to cellulose decomposition had significant stage correspondence.
34337038	0	24	theme	Microbial	9:17	arg1	Succession					29:38	Microbial Community Succession	9:38	Microbial Community Succession	9:38	Study on Microbial Community Succession and Functional Analysis during Biodegradation of Mushroom Residue.
34337038	1	25	theme	composting	296:305	arg1	stages					307:312	different composting stages	286:312	different composting stages	286:312	In this study, 16S rRNA high-throughput sequencing technology was used to analyze the composition and diversity of bacterial and fungal communities in mushroom residue samples at different composting stages.
34337038	4	26	theme	mass	861:864	arg1	spectrometry					866:877	mass spectrometry	861:877	mass spectrometry	861:877	Furthermore, in order to obtain the protein expressed in each stage of composting, the nonstandard quantitative method (label free) was used to analyze it quantitatively by mass spectrometry, anda total of 22815 proteins were identified.
34337038	5	27	theme	significant	1161:1171	arg1	correspondence					1179:1192	significant stage correspondence	1161:1192	significant stage correspondence	1161:1192	It indicated that the number of identified proteins related to cellulose decomposition and the number of differentially expressed proteins were significantly enriched, and the functional proteins related to cellulose decomposition had significant stage correspondence.
34337038	4	28	theme	anda	880:883	arg1	total					885:889	anda total	880:889	anda total of 22815 proteins	880:907	Furthermore, in order to obtain the protein expressed in each stage of composting, the nonstandard quantitative method (label free) was used to analyze it quantitatively by mass spectrometry, anda total of 22815 proteins were identified.
34337038	5	29	theme	functional	1102:1111	arg1	proteins					1113:1120	the functional proteins	1098:1120	the functional proteins related to cellulose decomposition	1098:1155	It indicated that the number of identified proteins related to cellulose decomposition and the number of differentially expressed proteins were significantly enriched, and the functional proteins related to cellulose decomposition had significant stage correspondence.
34337038	3	30	theme	composting	618:627	arg1	process					629:635	the composting process	614:635	the composting process	614:635	The results showed that Actinobacteria, Firmicutes, Proteobacteria, Bacteroidetes, and Chloroflexi were the main microorganisms in the composting process, accounting for 98.9%-99.7% of the total bacteria.
34337038	2	31	theme	temperature	422:432	arg1	50°C					440:443	the temperature above 50°C	418:443	the temperature above 50°C	418:443	During the composting process, the maximum temperature in the center of the pile can reach 52.4°C, and the temperature above 50°C has been maintained for about 8 days.
34337038	1	32	from	diversity	209:217	arg1	samples					275:281	mushroom residue samples	258:281	mushroom residue samples	258:281	In this study, 16S rRNA high-throughput sequencing technology was used to analyze the composition and diversity of bacterial and fungal communities in mushroom residue samples at different composting stages.
34337038	4	33	theme	quantitative	787:798	arg1	method					800:805	the nonstandard quantitative method	771:805	the nonstandard quantitative method (label free)	771:818	Furthermore, in order to obtain the protein expressed in each stage of composting, the nonstandard quantitative method (label free) was used to analyze it quantitatively by mass spectrometry, anda total of 22815 proteins were identified.
34337038	2	34	theme	maximum	350:356	arg1	temperature					358:368	the maximum temperature	346:368	the maximum temperature in the center of the pile	346:394	During the composting process, the maximum temperature in the center of the pile can reach 52.4°C, and the temperature above 50°C has been maintained for about 8 days.
34337038	0	35	theme	Functional	44:53	arg1	Analysis					55:62	Functional Analysis	44:62	Functional Analysis	44:62	Study on Microbial Community Succession and Functional Analysis during Biodegradation of Mushroom Residue.
34337038	5	36	theme	related	1122:1128	arg1	proteins					1113:1120	the functional proteins	1098:1120	the functional proteins related to cellulose decomposition	1098:1155	It indicated that the number of identified proteins related to cellulose decomposition and the number of differentially expressed proteins were significantly enriched, and the functional proteins related to cellulose decomposition had significant stage correspondence.
34337038	3	37	theme	%	657:657	arg1	%					663:663	98.9%-99.7%	653:663	98.9%-99.7% of the total bacteria	653:685	The results showed that Actinobacteria, Firmicutes, Proteobacteria, Bacteroidetes, and Chloroflexi were the main microorganisms in the composting process, accounting for 98.9%-99.7% of the total bacteria.
34337038	3	37	theme	%	657:657	arg1	bacteria					678:685	the total bacteria	668:685	the total bacteria	668:685	The results showed that Actinobacteria, Firmicutes, Proteobacteria, Bacteroidetes, and Chloroflexi were the main microorganisms in the composting process, accounting for 98.9%-99.7% of the total bacteria.
34337038	4	38	theme	nonstandard	775:785	arg1	method					800:805	the nonstandard quantitative method	771:805	the nonstandard quantitative method (label free)	771:818	Furthermore, in order to obtain the protein expressed in each stage of composting, the nonstandard quantitative method (label free) was used to analyze it quantitatively by mass spectrometry, anda total of 22815 proteins were identified.
34337038	1	39	from	composition	193:203	arg1	samples					275:281	mushroom residue samples	258:281	mushroom residue samples	258:281	In this study, 16S rRNA high-throughput sequencing technology was used to analyze the composition and diversity of bacterial and fungal communities in mushroom residue samples at different composting stages.
34337038	5	40	theme	cellulose	989:997	arg1	decomposition					999:1011	cellulose decomposition	989:1011	cellulose decomposition	989:1011	It indicated that the number of identified proteins related to cellulose decomposition and the number of differentially expressed proteins were significantly enriched, and the functional proteins related to cellulose decomposition had significant stage correspondence.
34337038	5	41	theme	identified	958:967	arg1	proteins					969:976	identified proteins	958:976	identified proteins related to cellulose decomposition	958:1011	It indicated that the number of identified proteins related to cellulose decomposition and the number of differentially expressed proteins were significantly enriched, and the functional proteins related to cellulose decomposition had significant stage correspondence.
34337038	1	42	theme	16S	122:124	arg1	rRNA					126:129	16S rRNA	122:129	16S rRNA high-throughput sequencing technology	122:167	In this study, 16S rRNA high-throughput sequencing technology was used to analyze the composition and diversity of bacterial and fungal communities in mushroom residue samples at different composting stages.
34337038	3	43	theme	main	591:594	arg1	Firmicutes					523:532	Firmicutes	523:532	Firmicutes	523:532	The results showed that Actinobacteria, Firmicutes, Proteobacteria, Bacteroidetes, and Chloroflexi were the main microorganisms in the composting process, accounting for 98.9%-99.7% of the total bacteria.
34337038	3	43	theme	main	591:594	arg1	microorganisms					596:609	the main microorganisms	587:609	the main microorganisms	587:609	The results showed that Actinobacteria, Firmicutes, Proteobacteria, Bacteroidetes, and Chloroflexi were the main microorganisms in the composting process, accounting for 98.9%-99.7% of the total bacteria.
34337038	1	44	theme	bacterial	222:230	arg1	communities					243:253	bacterial and fungal communities	222:253	bacterial and fungal communities	222:253	In this study, 16S rRNA high-throughput sequencing technology was used to analyze the composition and diversity of bacterial and fungal communities in mushroom residue samples at different composting stages.
34337038	1	45	theme	rRNA	126:129	arg1	technology					158:167	16S rRNA high-throughput sequencing technology	122:167	16S rRNA high-throughput sequencing technology	122:167	In this study, 16S rRNA high-throughput sequencing technology was used to analyze the composition and diversity of bacterial and fungal communities in mushroom residue samples at different composting stages.
34337038	3	46	dep	Firmicutes	523:532	arg1	Bacteroidetes					551:563	Bacteroidetes	551:563	Bacteroidetes	551:563	The results showed that Actinobacteria, Firmicutes, Proteobacteria, Bacteroidetes, and Chloroflexi were the main microorganisms in the composting process, accounting for 98.9%-99.7% of the total bacteria.
34337038	3	46	dep	Firmicutes	523:532	arg1	Chloroflexi					570:580	Chloroflexi	570:580	Chloroflexi	570:580	The results showed that Actinobacteria, Firmicutes, Proteobacteria, Bacteroidetes, and Chloroflexi were the main microorganisms in the composting process, accounting for 98.9%-99.7% of the total bacteria.
34337038	3	46	dep	Firmicutes	523:532	arg1	Proteobacteria					535:548	Proteobacteria	535:548	Proteobacteria	535:548	The results showed that Actinobacteria, Firmicutes, Proteobacteria, Bacteroidetes, and Chloroflexi were the main microorganisms in the composting process, accounting for 98.9%-99.7% of the total bacteria.
34337038	1	47	dep	composition	193:203	arg1	the					189:191	the	189:191	the	189:191	In this study, 16S rRNA high-throughput sequencing technology was used to analyze the composition and diversity of bacterial and fungal communities in mushroom residue samples at different composting stages.
34337038	1	48	theme	high-throughput	131:145	arg1	technology					158:167	16S rRNA high-throughput sequencing technology	122:167	16S rRNA high-throughput sequencing technology	122:167	In this study, 16S rRNA high-throughput sequencing technology was used to analyze the composition and diversity of bacterial and fungal communities in mushroom residue samples at different composting stages.
34337038	5	49	theme	cellulose	1133:1141	arg1	decomposition					1143:1155	cellulose decomposition	1133:1155	cellulose decomposition	1133:1155	It indicated that the number of identified proteins related to cellulose decomposition and the number of differentially expressed proteins were significantly enriched, and the functional proteins related to cellulose decomposition had significant stage correspondence.
34337038	1	50	theme	fungal	236:241	arg1	communities					243:253	bacterial and fungal communities	222:253	bacterial and fungal communities	222:253	In this study, 16S rRNA high-throughput sequencing technology was used to analyze the composition and diversity of bacterial and fungal communities in mushroom residue samples at different composting stages.
34337038	1	51	used	used	173:176	arg2	technology					158:167	16S rRNA high-throughput sequencing technology	122:167	16S rRNA high-throughput sequencing technology	122:167	In this study, 16S rRNA high-throughput sequencing technology was used to analyze the composition and diversity of bacterial and fungal communities in mushroom residue samples at different composting stages.
34337038	1	52	theme	sequencing	147:156	arg1	technology					158:167	16S rRNA high-throughput sequencing technology	122:167	16S rRNA high-throughput sequencing technology	122:167	In this study, 16S rRNA high-throughput sequencing technology was used to analyze the composition and diversity of bacterial and fungal communities in mushroom residue samples at different composting stages.
34337038	1	53	theme	communities	243:253	arg1	diversity					209:217	diversity	209:217	diversity	209:217	In this study, 16S rRNA high-throughput sequencing technology was used to analyze the composition and diversity of bacterial and fungal communities in mushroom residue samples at different composting stages.
34337038	1	53	theme	communities	243:253	arg1	composition					193:203	composition	193:203	composition	193:203	In this study, 16S rRNA high-throughput sequencing technology was used to analyze the composition and diversity of bacterial and fungal communities in mushroom residue samples at different composting stages.
34298051	7	0	link	tumor-derived	1353:1365	arg1	formation					1375:1383	tumor-derived exosome formation	1353:1383	tumor-derived exosome formation	1353:1383	Moreover, the sulfate group at 6-O position of heparan sulfate has been proved to play an important role in tumor-derived exosome formation and functions.
34298051	5	1	from	CD63	849:852	arg1	exosomes					886:893	exosomes	886:893	exosomes derived from heparin-induced B16F10 cells	886:935	The findings indicated the reduced tumor-derived exosome secretion and protein cargo as reflected by lower levels of CD63, TSG101, heparinase and IL-6 in exosomes derived from heparin-induced B16F10 cells as compared with 6-O-desulfated heparin-induced tumor cells.
34298051	2	2	theme	analogues	336:344	arg1	role					325:328	the role	321:328	the role of HS analogues (heparin, low molecular weight heparin, and 6-O-desulfated heparin) in modulating exosome secretion	321:444	This study investigated the role of HS analogues (heparin, low molecular weight heparin, and 6-O-desulfated heparin) in modulating exosome secretion, composition and functions.
34298051	2	2	theme	analogues	336:344	arg1	functions					463:471	functions	463:471	functions	463:471	This study investigated the role of HS analogues (heparin, low molecular weight heparin, and 6-O-desulfated heparin) in modulating exosome secretion, composition and functions.
34298051	2	2	theme	analogues	336:344	arg1	composition					447:457	composition	447:457	composition	447:457	This study investigated the role of HS analogues (heparin, low molecular weight heparin, and 6-O-desulfated heparin) in modulating exosome secretion, composition and functions.
34298051	7	3	theme	tumor-derived	1353:1365	arg1	formation					1375:1383	tumor-derived exosome formation	1353:1383	tumor-derived exosome formation	1353:1383	Moreover, the sulfate group at 6-O position of heparan sulfate has been proved to play an important role in tumor-derived exosome formation and functions.
34298051	6	4	theme	Further	998:1004	arg1	assays					1017:1022	Further functional assays	998:1022	Further functional assays	998:1022	Further functional assays demonstrated that exosomes from tumor cells exposed to heparin weakened tumor proliferation, migration and invasion most significantly among various exosomes derived from B16F10 cells treated with different HS analogues.
34298051	2	5	theme	weight	370:375	arg1	analogues					336:344	HS analogues	333:344	HS analogues (heparin, low molecular weight heparin, and 6-O-desulfated heparin)	333:412	This study investigated the role of HS analogues (heparin, low molecular weight heparin, and 6-O-desulfated heparin) in modulating exosome secretion, composition and functions.
34298051	2	5	theme	weight	370:375	arg1	heparin					377:383	low molecular weight heparin	356:383	low molecular weight heparin	356:383	This study investigated the role of HS analogues (heparin, low molecular weight heparin, and 6-O-desulfated heparin) in modulating exosome secretion, composition and functions.
34298051	1	6	dep	occurrence	259:268	arg1	the					255:257	the	255:257	the	255:257	Heparan sulfate (HS) is involved in many biological activities, including the biogenesis and uptake of exosomes, which are related to the occurrence and development of tumors.
34298051	1	7	theme	many	157:160	arg1	biogenesis					199:208	biogenesis	199:208	biogenesis	199:208	Heparan sulfate (HS) is involved in many biological activities, including the biogenesis and uptake of exosomes, which are related to the occurrence and development of tumors.
34298051	1	7	theme	many	157:160	arg1	activities					173:182	many biological activities	157:182	many biological activities	157:182	Heparan sulfate (HS) is involved in many biological activities, including the biogenesis and uptake of exosomes, which are related to the occurrence and development of tumors.
34298051	1	7	theme	many	157:160	arg1	uptake					214:219	uptake	214:219	uptake	214:219	Heparan sulfate (HS) is involved in many biological activities, including the biogenesis and uptake of exosomes, which are related to the occurrence and development of tumors.
34298051	8	8	theme	tumor-derived	1527:1539	arg1	exosomes					1541:1548	tumor-derived exosomes	1527:1548	tumor-derived exosomes	1527:1548	This study suggested a vital view to develop more specific and efficient HS-based strategies in cancer treatment for targeting tumor-derived exosomes.
34298051	5	9	attach	derived	895:901	arg1	cells					931:935	heparin-induced B16F10 cells	908:935	heparin-induced B16F10 cells	908:935	The findings indicated the reduced tumor-derived exosome secretion and protein cargo as reflected by lower levels of CD63, TSG101, heparinase and IL-6 in exosomes derived from heparin-induced B16F10 cells as compared with 6-O-desulfated heparin-induced tumor cells.
34298051	5	9	attach	derived	895:901	arg2	exosomes					886:893	exosomes	886:893	exosomes derived from heparin-induced B16F10 cells	886:935	The findings indicated the reduced tumor-derived exosome secretion and protein cargo as reflected by lower levels of CD63, TSG101, heparinase and IL-6 in exosomes derived from heparin-induced B16F10 cells as compared with 6-O-desulfated heparin-induced tumor cells.
34298051	2	10	theme	HS	333:334	arg1	analogues					336:344	HS analogues	333:344	HS analogues (heparin, low molecular weight heparin, and 6-O-desulfated heparin)	333:412	This study investigated the role of HS analogues (heparin, low molecular weight heparin, and 6-O-desulfated heparin) in modulating exosome secretion, composition and functions.
34298051	2	10	theme	HS	333:334	arg1	heparin					405:411	6-O-desulfated heparin	390:411	6-O-desulfated heparin	390:411	This study investigated the role of HS analogues (heparin, low molecular weight heparin, and 6-O-desulfated heparin) in modulating exosome secretion, composition and functions.
34298051	2	10	theme	HS	333:334	arg1	heparin					377:383	low molecular weight heparin	356:383	low molecular weight heparin	356:383	This study investigated the role of HS analogues (heparin, low molecular weight heparin, and 6-O-desulfated heparin) in modulating exosome secretion, composition and functions.
34298051	2	10	theme	HS	333:334	arg1	heparin					347:353	heparin	347:353	heparin	347:353	This study investigated the role of HS analogues (heparin, low molecular weight heparin, and 6-O-desulfated heparin) in modulating exosome secretion, composition and functions.
34298051	5	11	from	IL-6	878:881	arg1	exosomes					886:893	exosomes	886:893	exosomes derived from heparin-induced B16F10 cells	886:935	The findings indicated the reduced tumor-derived exosome secretion and protein cargo as reflected by lower levels of CD63, TSG101, heparinase and IL-6 in exosomes derived from heparin-induced B16F10 cells as compared with 6-O-desulfated heparin-induced tumor cells.
34298051	2	12	dep	analogues	336:344	arg1	analogues					336:344	HS analogues	333:344	HS analogues (heparin, low molecular weight heparin, and 6-O-desulfated heparin)	333:412	This study investigated the role of HS analogues (heparin, low molecular weight heparin, and 6-O-desulfated heparin) in modulating exosome secretion, composition and functions.
34298051	2	12	dep	analogues	336:344	arg1	heparin					405:411	6-O-desulfated heparin	390:411	6-O-desulfated heparin	390:411	This study investigated the role of HS analogues (heparin, low molecular weight heparin, and 6-O-desulfated heparin) in modulating exosome secretion, composition and functions.
34298051	2	12	dep	analogues	336:344	arg1	heparin					377:383	low molecular weight heparin	356:383	low molecular weight heparin	356:383	This study investigated the role of HS analogues (heparin, low molecular weight heparin, and 6-O-desulfated heparin) in modulating exosome secretion, composition and functions.
34298051	2	12	dep	analogues	336:344	arg1	heparin					347:353	heparin	347:353	heparin	347:353	This study investigated the role of HS analogues (heparin, low molecular weight heparin, and 6-O-desulfated heparin) in modulating exosome secretion, composition and functions.
34298051	0	13	theme	tumor	104:108	arg1	processes					110:118	tumor processes	104:118	tumor processes	104:118	Heparan sulfate analogues regulate tumor-derived exosome formation that attenuates exosome functions in tumor processes.
34298051	6	14	theme	HS	1231:1232	arg1	analogues					1234:1242	different HS analogues	1221:1242	different HS analogues	1221:1242	Further functional assays demonstrated that exosomes from tumor cells exposed to heparin weakened tumor proliferation, migration and invasion most significantly among various exosomes derived from B16F10 cells treated with different HS analogues.
34298051	3	15	theme	HS	530:531	arg1	analogues					533:541	different HS analogues	520:541	different HS analogues	520:541	Exosomes derived from B16F10 cells exposed to different HS analogues were isolated and characterized by TEM, western blotting and Nanosight analyses.
34298051	4	16	theme	exosomes	662:669	arg1	size					636:639	size	636:639	size	636:639	The number, size and protein cargo of exosomes secreted by HS analogues-induced B16F10 cells were detected.
34298051	4	16	theme	exosomes	662:669	arg1	cargo					653:657	protein cargo	645:657	protein cargo	645:657	The number, size and protein cargo of exosomes secreted by HS analogues-induced B16F10 cells were detected.
34298051	4	16	theme	exosomes	662:669	arg1	number					628:633	number	628:633	number	628:633	The number, size and protein cargo of exosomes secreted by HS analogues-induced B16F10 cells were detected.
34298051	7	17	theme	heparan	1292:1298	arg1	sulfate					1300:1306	heparan sulfate	1292:1306	heparan sulfate	1292:1306	Moreover, the sulfate group at 6-O position of heparan sulfate has been proved to play an important role in tumor-derived exosome formation and functions.
34298051	5	18	from	heparinase	863:872	arg1	exosomes					886:893	exosomes	886:893	exosomes derived from heparin-induced B16F10 cells	886:935	The findings indicated the reduced tumor-derived exosome secretion and protein cargo as reflected by lower levels of CD63, TSG101, heparinase and IL-6 in exosomes derived from heparin-induced B16F10 cells as compared with 6-O-desulfated heparin-induced tumor cells.
34298051	5	19	theme	6-O-desulfated	954:967	arg1	cells					991:995	6-O-desulfated heparin-induced tumor cells	954:995	6-O-desulfated heparin-induced tumor cells	954:995	The findings indicated the reduced tumor-derived exosome secretion and protein cargo as reflected by lower levels of CD63, TSG101, heparinase and IL-6 in exosomes derived from heparin-induced B16F10 cells as compared with 6-O-desulfated heparin-induced tumor cells.
34298051	4	20	theme	protein	645:651	arg1	cargo					653:657	protein cargo	645:657	protein cargo	645:657	The number, size and protein cargo of exosomes secreted by HS analogues-induced B16F10 cells were detected.
34298051	1	21	theme	biological	162:171	arg1	biogenesis					199:208	biogenesis	199:208	biogenesis	199:208	Heparan sulfate (HS) is involved in many biological activities, including the biogenesis and uptake of exosomes, which are related to the occurrence and development of tumors.
34298051	1	21	theme	biological	162:171	arg1	activities					173:182	many biological activities	157:182	many biological activities	157:182	Heparan sulfate (HS) is involved in many biological activities, including the biogenesis and uptake of exosomes, which are related to the occurrence and development of tumors.
34298051	1	21	theme	biological	162:171	arg1	uptake					214:219	uptake	214:219	uptake	214:219	Heparan sulfate (HS) is involved in many biological activities, including the biogenesis and uptake of exosomes, which are related to the occurrence and development of tumors.
34298051	8	22	from	strategies	1482:1491	arg1	treatment					1503:1511	cancer treatment	1496:1511	cancer treatment	1496:1511	This study suggested a vital view to develop more specific and efficient HS-based strategies in cancer treatment for targeting tumor-derived exosomes.
34298051	5	23	theme	IL-6	878:881	arg1	levels					839:844	lower levels	833:844	lower levels of CD63, TSG101, heparinase and IL-6 in exosomes derived from heparin-induced B16F10 cells	833:935	The findings indicated the reduced tumor-derived exosome secretion and protein cargo as reflected by lower levels of CD63, TSG101, heparinase and IL-6 in exosomes derived from heparin-induced B16F10 cells as compared with 6-O-desulfated heparin-induced tumor cells.
34298051	5	24	theme	reduced	759:765	arg1	secretion					789:797	the reduced tumor-derived exosome secretion	755:797	the reduced tumor-derived exosome secretion	755:797	The findings indicated the reduced tumor-derived exosome secretion and protein cargo as reflected by lower levels of CD63, TSG101, heparinase and IL-6 in exosomes derived from heparin-induced B16F10 cells as compared with 6-O-desulfated heparin-induced tumor cells.
34298051	7	25	from	position	1280:1287	arg1	group					1267:1271	the sulfate group	1255:1271	the sulfate group at 6-O position of heparan sulfate	1255:1306	Moreover, the sulfate group at 6-O position of heparan sulfate has been proved to play an important role in tumor-derived exosome formation and functions.
34298051	5	26	theme	protein	803:809	arg1	cargo					811:815	protein cargo	803:815	protein cargo	803:815	The findings indicated the reduced tumor-derived exosome secretion and protein cargo as reflected by lower levels of CD63, TSG101, heparinase and IL-6 in exosomes derived from heparin-induced B16F10 cells as compared with 6-O-desulfated heparin-induced tumor cells.
34298051	6	27	theme	tumor	1096:1100	arg1	proliferation					1102:1114	tumor proliferation	1096:1114	tumor proliferation	1096:1114	Further functional assays demonstrated that exosomes from tumor cells exposed to heparin weakened tumor proliferation, migration and invasion most significantly among various exosomes derived from B16F10 cells treated with different HS analogues.
34298051	2	28	theme	molecular	360:368	arg1	analogues					336:344	HS analogues	333:344	HS analogues (heparin, low molecular weight heparin, and 6-O-desulfated heparin)	333:412	This study investigated the role of HS analogues (heparin, low molecular weight heparin, and 6-O-desulfated heparin) in modulating exosome secretion, composition and functions.
34298051	2	28	theme	molecular	360:368	arg1	heparin					377:383	low molecular weight heparin	356:383	low molecular weight heparin	356:383	This study investigated the role of HS analogues (heparin, low molecular weight heparin, and 6-O-desulfated heparin) in modulating exosome secretion, composition and functions.
34298051	0	29	theme	sulfate	8:14	arg1	analogues					16:24	Heparan sulfate analogues	0:24	Heparan sulfate analogues	0:24	Heparan sulfate analogues regulate tumor-derived exosome formation that attenuates exosome functions in tumor processes.
34298051	7	30	theme	6-O	1276:1278	arg1	position					1280:1287	6-O position	1276:1287	6-O position of heparan sulfate	1276:1306	Moreover, the sulfate group at 6-O position of heparan sulfate has been proved to play an important role in tumor-derived exosome formation and functions.
34298051	5	31	theme	heparin-induced	908:922	arg1	cells					931:935	heparin-induced B16F10 cells	908:935	heparin-induced B16F10 cells	908:935	The findings indicated the reduced tumor-derived exosome secretion and protein cargo as reflected by lower levels of CD63, TSG101, heparinase and IL-6 in exosomes derived from heparin-induced B16F10 cells as compared with 6-O-desulfated heparin-induced tumor cells.
34298051	2	32	theme	low	356:358	arg1	analogues					336:344	HS analogues	333:344	HS analogues (heparin, low molecular weight heparin, and 6-O-desulfated heparin)	333:412	This study investigated the role of HS analogues (heparin, low molecular weight heparin, and 6-O-desulfated heparin) in modulating exosome secretion, composition and functions.
34298051	2	32	theme	low	356:358	arg1	heparin					377:383	low molecular weight heparin	356:383	low molecular weight heparin	356:383	This study investigated the role of HS analogues (heparin, low molecular weight heparin, and 6-O-desulfated heparin) in modulating exosome secretion, composition and functions.
34298051	0	33	theme	Heparan	0:6	arg1	sulfate					8:14	Heparan sulfate	0:14	Heparan sulfate analogues	0:24	Heparan sulfate analogues regulate tumor-derived exosome formation that attenuates exosome functions in tumor processes.
34298051	6	34	from	cells	1062:1066	arg1	exosomes					1042:1049	exosomes	1042:1049	exosomes from tumor cells exposed to heparin	1042:1085	Further functional assays demonstrated that exosomes from tumor cells exposed to heparin weakened tumor proliferation, migration and invasion most significantly among various exosomes derived from B16F10 cells treated with different HS analogues.
34298051	5	35	theme	B16F10	924:929	arg1	cells					931:935	heparin-induced B16F10 cells	908:935	heparin-induced B16F10 cells	908:935	The findings indicated the reduced tumor-derived exosome secretion and protein cargo as reflected by lower levels of CD63, TSG101, heparinase and IL-6 in exosomes derived from heparin-induced B16F10 cells as compared with 6-O-desulfated heparin-induced tumor cells.
34298051	5	36	theme	TSG101	855:860	arg1	levels					839:844	lower levels	833:844	lower levels of CD63, TSG101, heparinase and IL-6 in exosomes derived from heparin-induced B16F10 cells	833:935	The findings indicated the reduced tumor-derived exosome secretion and protein cargo as reflected by lower levels of CD63, TSG101, heparinase and IL-6 in exosomes derived from heparin-induced B16F10 cells as compared with 6-O-desulfated heparin-induced tumor cells.
34298051	8	37	theme	HS-based	1473:1480	arg1	strategies					1482:1491	more specific and efficient HS-based strategies	1445:1491	more specific and efficient HS-based strategies in cancer treatment for targeting tumor-derived exosomes	1445:1548	This study suggested a vital view to develop more specific and efficient HS-based strategies in cancer treatment for targeting tumor-derived exosomes.
34298051	6	38	theme	different	1221:1229	arg1	analogues					1234:1242	different HS analogues	1221:1242	different HS analogues	1221:1242	Further functional assays demonstrated that exosomes from tumor cells exposed to heparin weakened tumor proliferation, migration and invasion most significantly among various exosomes derived from B16F10 cells treated with different HS analogues.
34298051	0	39	link	tumor-derived	35:47	arg1	formation					57:65	tumor-derived exosome formation	35:65	tumor-derived exosome formation that attenuates exosome functions in tumor processes	35:118	Heparan sulfate analogues regulate tumor-derived exosome formation that attenuates exosome functions in tumor processes.
34298051	6	40	theme	various	1165:1171	arg1	exosomes					1173:1180	various exosomes	1165:1180	various exosomes derived from B16F10 cells treated with different HS analogues	1165:1242	Further functional assays demonstrated that exosomes from tumor cells exposed to heparin weakened tumor proliferation, migration and invasion most significantly among various exosomes derived from B16F10 cells treated with different HS analogues.
34298051	2	41	theme	6-O-desulfated	390:403	arg1	analogues					336:344	HS analogues	333:344	HS analogues (heparin, low molecular weight heparin, and 6-O-desulfated heparin)	333:412	This study investigated the role of HS analogues (heparin, low molecular weight heparin, and 6-O-desulfated heparin) in modulating exosome secretion, composition and functions.
34298051	2	41	theme	6-O-desulfated	390:403	arg1	heparin					405:411	6-O-desulfated heparin	390:411	6-O-desulfated heparin	390:411	This study investigated the role of HS analogues (heparin, low molecular weight heparin, and 6-O-desulfated heparin) in modulating exosome secretion, composition and functions.
34298051	6	42	attach	derived	1182:1188	arg1	cells					1202:1206	B16F10 cells	1195:1206	B16F10 cells treated with different HS analogues	1195:1242	Further functional assays demonstrated that exosomes from tumor cells exposed to heparin weakened tumor proliferation, migration and invasion most significantly among various exosomes derived from B16F10 cells treated with different HS analogues.
34298051	6	42	attach	derived	1182:1188	arg2	exosomes					1173:1180	various exosomes	1165:1180	various exosomes derived from B16F10 cells treated with different HS analogues	1165:1242	Further functional assays demonstrated that exosomes from tumor cells exposed to heparin weakened tumor proliferation, migration and invasion most significantly among various exosomes derived from B16F10 cells treated with different HS analogues.
34298051	6	43	theme	B16F10	1195:1200	arg1	cells					1202:1206	B16F10 cells	1195:1206	B16F10 cells treated with different HS analogues	1195:1242	Further functional assays demonstrated that exosomes from tumor cells exposed to heparin weakened tumor proliferation, migration and invasion most significantly among various exosomes derived from B16F10 cells treated with different HS analogues.
34298051	1	44	theme	Heparan	121:127	arg1	HS					138:139	HS	138:139	HS	138:139	Heparan sulfate (HS) is involved in many biological activities, including the biogenesis and uptake of exosomes, which are related to the occurrence and development of tumors.
34298051	1	44	theme	Heparan	121:127	arg1	sulfate					129:135	Heparan sulfate	121:135	Heparan sulfate (HS)	121:140	Heparan sulfate (HS) is involved in many biological activities, including the biogenesis and uptake of exosomes, which are related to the occurrence and development of tumors.
34298051	6	45	theme	tumor	1056:1060	arg1	cells					1062:1066	tumor cells	1056:1066	tumor cells exposed to heparin	1056:1085	Further functional assays demonstrated that exosomes from tumor cells exposed to heparin weakened tumor proliferation, migration and invasion most significantly among various exosomes derived from B16F10 cells treated with different HS analogues.
34298051	0	46	theme	exosome	49:55	arg1	formation					57:65	tumor-derived exosome formation	35:65	tumor-derived exosome formation that attenuates exosome functions in tumor processes	35:118	Heparan sulfate analogues regulate tumor-derived exosome formation that attenuates exosome functions in tumor processes.
34298051	8	47	link	tumor-derived	1527:1539	arg1	exosomes					1541:1548	tumor-derived exosomes	1527:1548	tumor-derived exosomes	1527:1548	This study suggested a vital view to develop more specific and efficient HS-based strategies in cancer treatment for targeting tumor-derived exosomes.
34298051	8	48	theme	specific	1450:1457	arg1	strategies					1482:1491	more specific and efficient HS-based strategies	1445:1491	more specific and efficient HS-based strategies in cancer treatment for targeting tumor-derived exosomes	1445:1548	This study suggested a vital view to develop more specific and efficient HS-based strategies in cancer treatment for targeting tumor-derived exosomes.
34298051	4	49	dep	number	628:633	arg1	The					624:626	The	624:626	The	624:626	The number, size and protein cargo of exosomes secreted by HS analogues-induced B16F10 cells were detected.
34298051	5	50	theme	lower	833:837	arg1	levels					839:844	lower levels	833:844	lower levels of CD63, TSG101, heparinase and IL-6 in exosomes derived from heparin-induced B16F10 cells	833:935	The findings indicated the reduced tumor-derived exosome secretion and protein cargo as reflected by lower levels of CD63, TSG101, heparinase and IL-6 in exosomes derived from heparin-induced B16F10 cells as compared with 6-O-desulfated heparin-induced tumor cells.
34298051	0	51	theme	tumor-derived	35:47	arg1	formation					57:65	tumor-derived exosome formation	35:65	tumor-derived exosome formation that attenuates exosome functions in tumor processes	35:118	Heparan sulfate analogues regulate tumor-derived exosome formation that attenuates exosome functions in tumor processes.
34298051	1	52	theme	tumors	289:294	arg1	development					274:284	development	274:284	development	274:284	Heparan sulfate (HS) is involved in many biological activities, including the biogenesis and uptake of exosomes, which are related to the occurrence and development of tumors.
34298051	1	52	theme	tumors	289:294	arg1	occurrence					259:268	occurrence	259:268	occurrence	259:268	Heparan sulfate (HS) is involved in many biological activities, including the biogenesis and uptake of exosomes, which are related to the occurrence and development of tumors.
34298051	5	53	theme	tumor-derived	767:779	arg1	secretion					789:797	the reduced tumor-derived exosome secretion	755:797	the reduced tumor-derived exosome secretion	755:797	The findings indicated the reduced tumor-derived exosome secretion and protein cargo as reflected by lower levels of CD63, TSG101, heparinase and IL-6 in exosomes derived from heparin-induced B16F10 cells as compared with 6-O-desulfated heparin-induced tumor cells.
34298051	7	54	theme	important	1335:1343	arg1	role					1345:1348	an important role	1332:1348	an important role	1332:1348	Moreover, the sulfate group at 6-O position of heparan sulfate has been proved to play an important role in tumor-derived exosome formation and functions.
34298051	5	55	theme	exosome	781:787	arg1	secretion					789:797	the reduced tumor-derived exosome secretion	755:797	the reduced tumor-derived exosome secretion	755:797	The findings indicated the reduced tumor-derived exosome secretion and protein cargo as reflected by lower levels of CD63, TSG101, heparinase and IL-6 in exosomes derived from heparin-induced B16F10 cells as compared with 6-O-desulfated heparin-induced tumor cells.
34298051	8	56	theme	efficient	1463:1471	arg1	strategies					1482:1491	more specific and efficient HS-based strategies	1445:1491	more specific and efficient HS-based strategies in cancer treatment for targeting tumor-derived exosomes	1445:1548	This study suggested a vital view to develop more specific and efficient HS-based strategies in cancer treatment for targeting tumor-derived exosomes.
34298051	8	57	theme	vital	1423:1427	arg1	view					1429:1432	a vital view	1421:1432	a vital view to develop more specific and efficient HS-based strategies in cancer treatment for targeting tumor-derived exosomes	1421:1548	This study suggested a vital view to develop more specific and efficient HS-based strategies in cancer treatment for targeting tumor-derived exosomes.
34298051	5	58	from	levels	839:844	arg1	exosomes					886:893	exosomes	886:893	exosomes derived from heparin-induced B16F10 cells	886:935	The findings indicated the reduced tumor-derived exosome secretion and protein cargo as reflected by lower levels of CD63, TSG101, heparinase and IL-6 in exosomes derived from heparin-induced B16F10 cells as compared with 6-O-desulfated heparin-induced tumor cells.
34298051	4	59	theme	HS	683:684	arg1	cells					711:715	HS analogues-induced B16F10 cells	683:715	HS analogues-induced B16F10 cells	683:715	The number, size and protein cargo of exosomes secreted by HS analogues-induced B16F10 cells were detected.
34298051	2	60	theme	exosome	428:434	arg1	secretion					436:444	exosome secretion	428:444	exosome secretion	428:444	This study investigated the role of HS analogues (heparin, low molecular weight heparin, and 6-O-desulfated heparin) in modulating exosome secretion, composition and functions.
34298051	5	61	link	tumor-derived	767:779	arg1	secretion					789:797	the reduced tumor-derived exosome secretion	755:797	the reduced tumor-derived exosome secretion	755:797	The findings indicated the reduced tumor-derived exosome secretion and protein cargo as reflected by lower levels of CD63, TSG101, heparinase and IL-6 in exosomes derived from heparin-induced B16F10 cells as compared with 6-O-desulfated heparin-induced tumor cells.
34298051	7	62	theme	sulfate	1300:1306	arg1	position					1280:1287	6-O position	1276:1287	6-O position of heparan sulfate	1276:1306	Moreover, the sulfate group at 6-O position of heparan sulfate has been proved to play an important role in tumor-derived exosome formation and functions.
34298051	5	63	from	exosomes	886:893	arg1	levels					839:844	lower levels	833:844	lower levels of CD63, TSG101, heparinase and IL-6 in exosomes derived from heparin-induced B16F10 cells	833:935	The findings indicated the reduced tumor-derived exosome secretion and protein cargo as reflected by lower levels of CD63, TSG101, heparinase and IL-6 in exosomes derived from heparin-induced B16F10 cells as compared with 6-O-desulfated heparin-induced tumor cells.
34298051	7	64	theme	exosome	1367:1373	arg1	formation					1375:1383	tumor-derived exosome formation	1353:1383	tumor-derived exosome formation	1353:1383	Moreover, the sulfate group at 6-O position of heparan sulfate has been proved to play an important role in tumor-derived exosome formation and functions.
34298051	5	65	theme	tumor	985:989	arg1	cells					991:995	6-O-desulfated heparin-induced tumor cells	954:995	6-O-desulfated heparin-induced tumor cells	954:995	The findings indicated the reduced tumor-derived exosome secretion and protein cargo as reflected by lower levels of CD63, TSG101, heparinase and IL-6 in exosomes derived from heparin-induced B16F10 cells as compared with 6-O-desulfated heparin-induced tumor cells.
34298051	3	66	attach	derived	483:489	arg2	Exosomes					474:481	Exosomes	474:481	Exosomes derived from B16F10 cells exposed to different HS analogues	474:541	Exosomes derived from B16F10 cells exposed to different HS analogues were isolated and characterized by TEM, western blotting and Nanosight analyses.
34298051	3	66	attach	derived	483:489	arg1	cells					503:507	B16F10 cells	496:507	B16F10 cells exposed to different HS analogues	496:541	Exosomes derived from B16F10 cells exposed to different HS analogues were isolated and characterized by TEM, western blotting and Nanosight analyses.
34298051	4	67	theme	B16F10	704:709	arg1	cells					711:715	HS analogues-induced B16F10 cells	683:715	HS analogues-induced B16F10 cells	683:715	The number, size and protein cargo of exosomes secreted by HS analogues-induced B16F10 cells were detected.
34298051	5	68	theme	heparin-induced	969:983	arg1	cells					991:995	6-O-desulfated heparin-induced tumor cells	954:995	6-O-desulfated heparin-induced tumor cells	954:995	The findings indicated the reduced tumor-derived exosome secretion and protein cargo as reflected by lower levels of CD63, TSG101, heparinase and IL-6 in exosomes derived from heparin-induced B16F10 cells as compared with 6-O-desulfated heparin-induced tumor cells.
34298051	3	69	theme	Nanosight	604:612	arg1	analyses					614:621	Nanosight analyses	604:621	Nanosight analyses	604:621	Exosomes derived from B16F10 cells exposed to different HS analogues were isolated and characterized by TEM, western blotting and Nanosight analyses.
34298051	4	70	theme	analogues-induced	686:702	arg1	cells					711:715	HS analogues-induced B16F10 cells	683:715	HS analogues-induced B16F10 cells	683:715	The number, size and protein cargo of exosomes secreted by HS analogues-induced B16F10 cells were detected.
34298051	1	71	theme	exosomes	224:231	arg1	biogenesis					199:208	biogenesis	199:208	biogenesis	199:208	Heparan sulfate (HS) is involved in many biological activities, including the biogenesis and uptake of exosomes, which are related to the occurrence and development of tumors.
34298051	1	71	theme	exosomes	224:231	arg1	uptake					214:219	uptake	214:219	uptake	214:219	Heparan sulfate (HS) is involved in many biological activities, including the biogenesis and uptake of exosomes, which are related to the occurrence and development of tumors.
34298051	0	72	theme	exosome	83:89	arg1	functions					91:99	exosome functions	83:99	exosome functions	83:99	Heparan sulfate analogues regulate tumor-derived exosome formation that attenuates exosome functions in tumor processes.
34298051	8	73	theme	cancer	1496:1501	arg1	treatment					1503:1511	cancer treatment	1496:1511	cancer treatment	1496:1511	This study suggested a vital view to develop more specific and efficient HS-based strategies in cancer treatment for targeting tumor-derived exosomes.
34298051	3	74	theme	western	583:589	arg1	blotting					591:598	western blotting	583:598	western blotting	583:598	Exosomes derived from B16F10 cells exposed to different HS analogues were isolated and characterized by TEM, western blotting and Nanosight analyses.
34298051	5	75	from	TSG101	855:860	arg1	exosomes					886:893	exosomes	886:893	exosomes derived from heparin-induced B16F10 cells	886:935	The findings indicated the reduced tumor-derived exosome secretion and protein cargo as reflected by lower levels of CD63, TSG101, heparinase and IL-6 in exosomes derived from heparin-induced B16F10 cells as compared with 6-O-desulfated heparin-induced tumor cells.
34298051	3	76	theme	B16F10	496:501	arg1	cells					503:507	B16F10 cells	496:507	B16F10 cells exposed to different HS analogues	496:541	Exosomes derived from B16F10 cells exposed to different HS analogues were isolated and characterized by TEM, western blotting and Nanosight analyses.
34298051	5	77	theme	CD63	849:852	arg1	levels					839:844	lower levels	833:844	lower levels of CD63, TSG101, heparinase and IL-6 in exosomes derived from heparin-induced B16F10 cells	833:935	The findings indicated the reduced tumor-derived exosome secretion and protein cargo as reflected by lower levels of CD63, TSG101, heparinase and IL-6 in exosomes derived from heparin-induced B16F10 cells as compared with 6-O-desulfated heparin-induced tumor cells.
34298051	3	78	theme	different	520:528	arg1	analogues					533:541	different HS analogues	520:541	different HS analogues	520:541	Exosomes derived from B16F10 cells exposed to different HS analogues were isolated and characterized by TEM, western blotting and Nanosight analyses.
34298051	1	79	dep	biogenesis	199:208	arg1	the					195:197	the	195:197	the	195:197	Heparan sulfate (HS) is involved in many biological activities, including the biogenesis and uptake of exosomes, which are related to the occurrence and development of tumors.
34298051	5	80	theme	heparinase	863:872	arg1	levels					839:844	lower levels	833:844	lower levels of CD63, TSG101, heparinase and IL-6 in exosomes derived from heparin-induced B16F10 cells	833:935	The findings indicated the reduced tumor-derived exosome secretion and protein cargo as reflected by lower levels of CD63, TSG101, heparinase and IL-6 in exosomes derived from heparin-induced B16F10 cells as compared with 6-O-desulfated heparin-induced tumor cells.
34298051	7	81	theme	sulfate	1259:1265	arg1	group					1267:1271	the sulfate group	1255:1271	the sulfate group at 6-O position of heparan sulfate	1255:1306	Moreover, the sulfate group at 6-O position of heparan sulfate has been proved to play an important role in tumor-derived exosome formation and functions.
34298051	6	82	theme	functional	1006:1015	arg1	assays					1017:1022	Further functional assays	998:1022	Further functional assays	998:1022	Further functional assays demonstrated that exosomes from tumor cells exposed to heparin weakened tumor proliferation, migration and invasion most significantly among various exosomes derived from B16F10 cells treated with different HS analogues.
33966848	0	0	theme	beads	76:80	arg1	fabrication					7:17	Facile fabrication	0:17	Facile fabrication of novel magnetic ZIF-67 MOF@aminated chitosan composite beads for the adsorptive removal of Cr(VI) from aqueous solutions.	0:141	Facile fabrication of novel magnetic ZIF-67 MOF@aminated chitosan composite beads for the adsorptive removal of Cr(VI) from aqueous solutions.
33966848	5	1	theme	Cr	729:730	arg1	ions					736:739	ions	736:739	toxic Cr(VI) ions	723:739	The fabricated magnetic composite beads were examined their adsorptive removal aptitude towards toxic Cr(VI) ions.
33966848	5	2	theme	magnetic	642:649	arg1	beads					661:665	The fabricated magnetic composite beads	627:665	The fabricated magnetic composite beads	627:665	The fabricated magnetic composite beads were examined their adsorptive removal aptitude towards toxic Cr(VI) ions.
33966848	0	3	dep	Cr	112:113	arg1	VI					115:116	VI	115:116	VI	115:116	Facile fabrication of novel magnetic ZIF-67 MOF@aminated chitosan composite beads for the adsorptive removal of Cr(VI) from aqueous solutions.
33966848	0	4	from	solutions	132:140	arg1	removal					101:107	the adsorptive removal	86:107	the adsorptive removal of Cr(VI) from aqueous solutions	86:140	Facile fabrication of novel magnetic ZIF-67 MOF@aminated chitosan composite beads for the adsorptive removal of Cr(VI) from aqueous solutions.
33966848	0	5	theme	composite	66:74	arg1	beads					76:80	novel magnetic ZIF-67 MOF@aminated chitosan composite beads	22:80	novel magnetic ZIF-67 MOF@aminated chitosan composite beads	22:80	Facile fabrication of novel magnetic ZIF-67 MOF@aminated chitosan composite beads for the adsorptive removal of Cr(VI) from aqueous solutions.
33966848	6	6	theme	AmCs	858:861	arg1	beads					873:877	magnetic Fe3O4/ZIF-67@AmCs composite beads	836:877	magnetic Fe3O4/ZIF-67@AmCs composite beads at 25 °C	836:886	The gained results refereed that a maximum adsorption capacity of 119.05 mg/g was attained by magnetic Fe3O4/ZIF-67@AmCs composite beads at 25 °C.
33966848	7	7	theme	kinetic	1000:1006	arg1	model					1008:1012	kinetic model	1000:1012	kinetic model	1000:1012	The process obeyed both of Langmuir and Freundlich isotherm models, and the pseudo 2nd order was more suitable kinetic model to represent the adsorption process.
33966848	6	8	theme	@	857:857	arg1	beads					873:877	magnetic Fe3O4/ZIF-67@AmCs composite beads	836:877	magnetic Fe3O4/ZIF-67@AmCs composite beads at 25 °C	836:886	The gained results refereed that a maximum adsorption capacity of 119.05 mg/g was attained by magnetic Fe3O4/ZIF-67@AmCs composite beads at 25 °C.
33966848	3	9	theme	shell	479:483	arg1	beads					513:517	core-dual shell Fe3O4/ZIF-67@AmCs composite beads	469:517	core-dual shell Fe3O4/ZIF-67@AmCs composite beads	469:517	In this study, ZIF-67 was decorated with magnetic Fe3O4 nanoparticles, and then embedded into aminated chitosan (AmCs) matrix to form core-dual shell Fe3O4/ZIF-67@AmCs composite beads.
33966848	4	10	theme	magnetic	601:608	arg1	beads					620:624	the magnetic composite beads	597:624	the magnetic composite beads	597:624	Diverse analysis tools were utilized to ensure the successful fabrication of the magnetic composite beads.
33966848	5	11	theme	adsorptive	687:696	arg1	aptitude					706:713	their adsorptive removal aptitude	681:713	their adsorptive removal aptitude towards toxic Cr(VI) ions	681:739	The fabricated magnetic composite beads were examined their adsorptive removal aptitude towards toxic Cr(VI) ions.
33966848	6	12	theme	Fe3O4/ZIF-67	845:856	arg1	beads					873:877	magnetic Fe3O4/ZIF-67@AmCs composite beads	836:877	magnetic Fe3O4/ZIF-67@AmCs composite beads at 25 °C	836:886	The gained results refereed that a maximum adsorption capacity of 119.05 mg/g was attained by magnetic Fe3O4/ZIF-67@AmCs composite beads at 25 °C.
33966848	6	13	theme	gained	746:751	arg1	results					753:759	The gained results	742:759	The gained results	742:759	The gained results refereed that a maximum adsorption capacity of 119.05 mg/g was attained by magnetic Fe3O4/ZIF-67@AmCs composite beads at 25 °C.
33966848	3	14	theme	Fe3O4/ZIF-67	485:496	arg1	beads					513:517	core-dual shell Fe3O4/ZIF-67@AmCs composite beads	469:517	core-dual shell Fe3O4/ZIF-67@AmCs composite beads	469:517	In this study, ZIF-67 was decorated with magnetic Fe3O4 nanoparticles, and then embedded into aminated chitosan (AmCs) matrix to form core-dual shell Fe3O4/ZIF-67@AmCs composite beads.
33966848	1	15	theme	hazardous	225:233	arg1	contaminants					235:246	hazardous contaminants	225:246	hazardous contaminants from wastewater	225:262	Metal organic frameworks (MOFs) have become premium candidates for the removal of hazardous contaminants from wastewater.
33966848	5	16	theme	removal	698:704	arg1	aptitude					706:713	their adsorptive removal aptitude	681:713	their adsorptive removal aptitude towards toxic Cr(VI) ions	681:739	The fabricated magnetic composite beads were examined their adsorptive removal aptitude towards toxic Cr(VI) ions.
33966848	6	17	theme	magnetic	836:843	arg1	beads					873:877	magnetic Fe3O4/ZIF-67@AmCs composite beads	836:877	magnetic Fe3O4/ZIF-67@AmCs composite beads at 25 °C	836:886	The gained results refereed that a maximum adsorption capacity of 119.05 mg/g was attained by magnetic Fe3O4/ZIF-67@AmCs composite beads at 25 °C.
33966848	1	18	from	wastewater	253:262	arg1	removal					214:220	the removal	210:220	the removal of hazardous contaminants from wastewater	210:262	Metal organic frameworks (MOFs) have become premium candidates for the removal of hazardous contaminants from wastewater.
33966848	1	18	from	wastewater	253:262	arg1	contaminants					235:246	hazardous contaminants	225:246	hazardous contaminants from wastewater	225:262	Metal organic frameworks (MOFs) have become premium candidates for the removal of hazardous contaminants from wastewater.
33966848	8	19	theme	Fe3O4/ZIF-67	1060:1071	arg1	composite					1078:1086	Fe3O4/ZIF-67@AmCs composite	1060:1086	Fe3O4/ZIF-67@AmCs composite	1060:1086	Besides, Fe3O4/ZIF-67@AmCs composite showed an excellent recyclability for the removal of Cr(VI) ions from their aqueous solutions for seven consecutive cycles.
33966848	8	20	dep	Cr	1141:1142	arg1	ions					1148:1151	ions	1148:1151	Cr(VI) ions	1141:1151	Besides, Fe3O4/ZIF-67@AmCs composite showed an excellent recyclability for the removal of Cr(VI) ions from their aqueous solutions for seven consecutive cycles.
33966848	8	20	dep	Cr	1141:1142	arg1	VI					1144:1145	VI	1144:1145	VI	1144:1145	Besides, Fe3O4/ZIF-67@AmCs composite showed an excellent recyclability for the removal of Cr(VI) ions from their aqueous solutions for seven consecutive cycles.
33966848	4	21	theme	beads	620:624	arg1	fabrication					582:592	the successful fabrication	567:592	the successful fabrication of the magnetic composite beads	567:624	Diverse analysis tools were utilized to ensure the successful fabrication of the magnetic composite beads.
33966848	3	22	theme	Fe3O4	385:389	arg1	nanoparticles					391:403	magnetic Fe3O4 nanoparticles	376:403	magnetic Fe3O4 nanoparticles	376:403	In this study, ZIF-67 was decorated with magnetic Fe3O4 nanoparticles, and then embedded into aminated chitosan (AmCs) matrix to form core-dual shell Fe3O4/ZIF-67@AmCs composite beads.
33966848	0	23	theme	adsorptive	90:99	arg1	removal					101:107	the adsorptive removal	86:107	the adsorptive removal of Cr(VI) from aqueous solutions	86:140	Facile fabrication of novel magnetic ZIF-67 MOF@aminated chitosan composite beads for the adsorptive removal of Cr(VI) from aqueous solutions.
33966848	4	24	theme	Diverse	520:526	arg1	tools					537:541	Diverse analysis tools	520:541	Diverse analysis tools	520:541	Diverse analysis tools were utilized to ensure the successful fabrication of the magnetic composite beads.
33966848	3	25	theme	core-dual	469:477	arg1	beads					513:517	core-dual shell Fe3O4/ZIF-67@AmCs composite beads	469:517	core-dual shell Fe3O4/ZIF-67@AmCs composite beads	469:517	In this study, ZIF-67 was decorated with magnetic Fe3O4 nanoparticles, and then embedded into aminated chitosan (AmCs) matrix to form core-dual shell Fe3O4/ZIF-67@AmCs composite beads.
33966848	1	26	theme	Metal	143:147	arg1	MOFs					169:172	MOFs	169:172	MOFs	169:172	Metal organic frameworks (MOFs) have become premium candidates for the removal of hazardous contaminants from wastewater.
33966848	1	26	theme	Metal	143:147	arg1	frameworks					157:166	Metal organic frameworks	143:166	Metal organic frameworks (MOFs)	143:173	Metal organic frameworks (MOFs) have become premium candidates for the removal of hazardous contaminants from wastewater.
33966848	8	27	theme	AmCs	1073:1076	arg1	composite					1078:1086	Fe3O4/ZIF-67@AmCs composite	1060:1086	Fe3O4/ZIF-67@AmCs composite	1060:1086	Besides, Fe3O4/ZIF-67@AmCs composite showed an excellent recyclability for the removal of Cr(VI) ions from their aqueous solutions for seven consecutive cycles.
33966848	8	28	theme	@	1072:1072	arg1	composite					1078:1086	Fe3O4/ZIF-67@AmCs composite	1060:1086	Fe3O4/ZIF-67@AmCs composite	1060:1086	Besides, Fe3O4/ZIF-67@AmCs composite showed an excellent recyclability for the removal of Cr(VI) ions from their aqueous solutions for seven consecutive cycles.
33966848	3	29	theme	composite	503:511	arg1	beads					513:517	core-dual shell Fe3O4/ZIF-67@AmCs composite beads	469:517	core-dual shell Fe3O4/ZIF-67@AmCs composite beads	469:517	In this study, ZIF-67 was decorated with magnetic Fe3O4 nanoparticles, and then embedded into aminated chitosan (AmCs) matrix to form core-dual shell Fe3O4/ZIF-67@AmCs composite beads.
33966848	1	30	theme	organic	149:155	arg1	MOFs					169:172	MOFs	169:172	MOFs	169:172	Metal organic frameworks (MOFs) have become premium candidates for the removal of hazardous contaminants from wastewater.
33966848	1	30	theme	organic	149:155	arg1	frameworks					157:166	Metal organic frameworks	143:166	Metal organic frameworks (MOFs)	143:173	Metal organic frameworks (MOFs) have become premium candidates for the removal of hazardous contaminants from wastewater.
33966848	0	31	theme	Facile	0:5	arg1	fabrication					7:17	Facile fabrication	0:17	Facile fabrication of novel magnetic ZIF-67 MOF@aminated chitosan composite beads for the adsorptive removal of Cr(VI) from aqueous solutions.	0:141	Facile fabrication of novel magnetic ZIF-67 MOF@aminated chitosan composite beads for the adsorptive removal of Cr(VI) from aqueous solutions.
33966848	1	32	theme	contaminants	235:246	arg1	removal					214:220	the removal	210:220	the removal of hazardous contaminants from wastewater	210:262	Metal organic frameworks (MOFs) have become premium candidates for the removal of hazardous contaminants from wastewater.
33966848	5	33	dep	ions	736:739	arg1	VI					732:733	VI	732:733	VI	732:733	The fabricated magnetic composite beads were examined their adsorptive removal aptitude towards toxic Cr(VI) ions.
33966848	3	34	theme	magnetic	376:383	arg1	nanoparticles					391:403	magnetic Fe3O4 nanoparticles	376:403	magnetic Fe3O4 nanoparticles	376:403	In this study, ZIF-67 was decorated with magnetic Fe3O4 nanoparticles, and then embedded into aminated chitosan (AmCs) matrix to form core-dual shell Fe3O4/ZIF-67@AmCs composite beads.
33966848	0	35	theme	novel	22:26	arg1	beads					76:80	novel magnetic ZIF-67 MOF@aminated chitosan composite beads	22:80	novel magnetic ZIF-67 MOF@aminated chitosan composite beads	22:80	Facile fabrication of novel magnetic ZIF-67 MOF@aminated chitosan composite beads for the adsorptive removal of Cr(VI) from aqueous solutions.
33966848	0	36	theme	Cr	112:113	arg1	removal					101:107	the adsorptive removal	86:107	the adsorptive removal of Cr(VI) from aqueous solutions	86:140	Facile fabrication of novel magnetic ZIF-67 MOF@aminated chitosan composite beads for the adsorptive removal of Cr(VI) from aqueous solutions.
33966848	3	37	theme	@	497:497	arg1	beads					513:517	core-dual shell Fe3O4/ZIF-67@AmCs composite beads	469:517	core-dual shell Fe3O4/ZIF-67@AmCs composite beads	469:517	In this study, ZIF-67 was decorated with magnetic Fe3O4 nanoparticles, and then embedded into aminated chitosan (AmCs) matrix to form core-dual shell Fe3O4/ZIF-67@AmCs composite beads.
33966848	4	38	theme	successful	571:580	arg1	fabrication					582:592	the successful fabrication	567:592	the successful fabrication of the magnetic composite beads	567:624	Diverse analysis tools were utilized to ensure the successful fabrication of the magnetic composite beads.
33966848	3	39	theme	AmCs	498:501	arg1	beads					513:517	core-dual shell Fe3O4/ZIF-67@AmCs composite beads	469:517	core-dual shell Fe3O4/ZIF-67@AmCs composite beads	469:517	In this study, ZIF-67 was decorated with magnetic Fe3O4 nanoparticles, and then embedded into aminated chitosan (AmCs) matrix to form core-dual shell Fe3O4/ZIF-67@AmCs composite beads.
33966848	6	40	theme	composite	863:871	arg1	beads					873:877	magnetic Fe3O4/ZIF-67@AmCs composite beads	836:877	magnetic Fe3O4/ZIF-67@AmCs composite beads at 25 °C	836:886	The gained results refereed that a maximum adsorption capacity of 119.05 mg/g was attained by magnetic Fe3O4/ZIF-67@AmCs composite beads at 25 °C.
33966848	0	41	theme	ZIF-67	37:42	arg1	beads					76:80	novel magnetic ZIF-67 MOF@aminated chitosan composite beads	22:80	novel magnetic ZIF-67 MOF@aminated chitosan composite beads	22:80	Facile fabrication of novel magnetic ZIF-67 MOF@aminated chitosan composite beads for the adsorptive removal of Cr(VI) from aqueous solutions.
33966848	6	42	theme	119.05 mg/g	808:818	arg1	capacity					796:803	a maximum adsorption capacity	775:803	a maximum adsorption capacity of 119.05 mg/g	775:818	The gained results refereed that a maximum adsorption capacity of 119.05 mg/g was attained by magnetic Fe3O4/ZIF-67@AmCs composite beads at 25 °C.
33966848	4	43	used	utilized	548:555	arg2	tools					537:541	Diverse analysis tools	520:541	Diverse analysis tools	520:541	Diverse analysis tools were utilized to ensure the successful fabrication of the magnetic composite beads.
33966848	4	44	theme	composite	610:618	arg1	beads					620:624	the magnetic composite beads	597:624	the magnetic composite beads	597:624	Diverse analysis tools were utilized to ensure the successful fabrication of the magnetic composite beads.
33966848	8	45	theme	consecutive	1192:1202	arg1	cycles					1204:1209	seven consecutive cycles	1186:1209	seven consecutive cycles	1186:1209	Besides, Fe3O4/ZIF-67@AmCs composite showed an excellent recyclability for the removal of Cr(VI) ions from their aqueous solutions for seven consecutive cycles.
33966848	0	46	theme	magnetic	28:35	arg1	beads					76:80	novel magnetic ZIF-67 MOF@aminated chitosan composite beads	22:80	novel magnetic ZIF-67 MOF@aminated chitosan composite beads	22:80	Facile fabrication of novel magnetic ZIF-67 MOF@aminated chitosan composite beads for the adsorptive removal of Cr(VI) from aqueous solutions.
33966848	1	47	from	removal	214:220	arg1	wastewater					253:262	wastewater	253:262	wastewater	253:262	Metal organic frameworks (MOFs) have become premium candidates for the removal of hazardous contaminants from wastewater.
33966848	7	48	theme	2nd	972:974	arg1	order					976:980	the pseudo 2nd order	961:980	the pseudo 2nd order	961:980	The process obeyed both of Langmuir and Freundlich isotherm models, and the pseudo 2nd order was more suitable kinetic model to represent the adsorption process.
33966848	7	48	theme	2nd	972:974	arg1	suitable					991:998	suitable	991:998	suitable	991:998	The process obeyed both of Langmuir and Freundlich isotherm models, and the pseudo 2nd order was more suitable kinetic model to represent the adsorption process.
33966848	8	49	theme	excellent	1098:1106	arg1	recyclability					1108:1120	an excellent recyclability	1095:1120	an excellent recyclability for the removal of Cr(VI) ions from their aqueous solutions for seven consecutive cycles	1095:1209	Besides, Fe3O4/ZIF-67@AmCs composite showed an excellent recyclability for the removal of Cr(VI) ions from their aqueous solutions for seven consecutive cycles.
33966848	0	50	theme	@	47:47	arg1	beads					76:80	novel magnetic ZIF-67 MOF@aminated chitosan composite beads	22:80	novel magnetic ZIF-67 MOF@aminated chitosan composite beads	22:80	Facile fabrication of novel magnetic ZIF-67 MOF@aminated chitosan composite beads for the adsorptive removal of Cr(VI) from aqueous solutions.
33966848	6	51	from	25 °C	882:886	arg1	beads					873:877	magnetic Fe3O4/ZIF-67@AmCs composite beads	836:877	magnetic Fe3O4/ZIF-67@AmCs composite beads at 25 °C	836:886	The gained results refereed that a maximum adsorption capacity of 119.05 mg/g was attained by magnetic Fe3O4/ZIF-67@AmCs composite beads at 25 °C.
33966848	5	52	theme	toxic	723:727	arg1	ions					736:739	ions	736:739	toxic Cr(VI) ions	723:739	The fabricated magnetic composite beads were examined their adsorptive removal aptitude towards toxic Cr(VI) ions.
33966848	7	53	theme	pseudo	965:970	arg1	order					976:980	the pseudo 2nd order	961:980	the pseudo 2nd order	961:980	The process obeyed both of Langmuir and Freundlich isotherm models, and the pseudo 2nd order was more suitable kinetic model to represent the adsorption process.
33966848	7	53	theme	pseudo	965:970	arg1	suitable					991:998	suitable	991:998	suitable	991:998	The process obeyed both of Langmuir and Freundlich isotherm models, and the pseudo 2nd order was more suitable kinetic model to represent the adsorption process.
33966848	3	54	theme	chitosan	438:445	arg1	matrix					454:459	aminated chitosan (AmCs) matrix	429:459	aminated chitosan (AmCs) matrix to form core-dual shell Fe3O4/ZIF-67@AmCs composite beads	429:517	In this study, ZIF-67 was decorated with magnetic Fe3O4 nanoparticles, and then embedded into aminated chitosan (AmCs) matrix to form core-dual shell Fe3O4/ZIF-67@AmCs composite beads.
33966848	0	55	theme	MOF	44:46	arg1	beads					76:80	novel magnetic ZIF-67 MOF@aminated chitosan composite beads	22:80	novel magnetic ZIF-67 MOF@aminated chitosan composite beads	22:80	Facile fabrication of novel magnetic ZIF-67 MOF@aminated chitosan composite beads for the adsorptive removal of Cr(VI) from aqueous solutions.
33966848	5	56	theme	composite	651:659	arg1	beads					661:665	The fabricated magnetic composite beads	627:665	The fabricated magnetic composite beads	627:665	The fabricated magnetic composite beads were examined their adsorptive removal aptitude towards toxic Cr(VI) ions.
33966848	6	57	theme	adsorption	785:794	arg1	capacity					796:803	a maximum adsorption capacity	775:803	a maximum adsorption capacity of 119.05 mg/g	775:818	The gained results refereed that a maximum adsorption capacity of 119.05 mg/g was attained by magnetic Fe3O4/ZIF-67@AmCs composite beads at 25 °C.
33966848	7	58	theme	adsorption	1031:1040	arg1	process					1042:1048	the adsorption process	1027:1048	the adsorption process	1027:1048	The process obeyed both of Langmuir and Freundlich isotherm models, and the pseudo 2nd order was more suitable kinetic model to represent the adsorption process.
33966848	8	59	theme	Cr	1141:1142	arg1	removal					1130:1136	the removal	1126:1136	the removal of Cr(VI) ions from their aqueous solutions	1126:1180	Besides, Fe3O4/ZIF-67@AmCs composite showed an excellent recyclability for the removal of Cr(VI) ions from their aqueous solutions for seven consecutive cycles.
33966848	2	60	contain	have	279:282	arg2	recyclability					320:332	their poor recyclability	309:332	their poor recyclability	309:332	However, MOFs have a vast obstacle which is their poor recyclability.
33966848	2	60	contain	have	279:282	arg1	MOFs					274:277	MOFs	274:277	MOFs	274:277	However, MOFs have a vast obstacle which is their poor recyclability.
33966848	2	60	contain	have	279:282	arg2	obstacle					291:298	a vast obstacle	284:298	a vast obstacle which is their poor recyclability	284:332	However, MOFs have a vast obstacle which is their poor recyclability.
33966848	5	61	theme	fabricated	631:640	arg1	beads					661:665	The fabricated magnetic composite beads	627:665	The fabricated magnetic composite beads	627:665	The fabricated magnetic composite beads were examined their adsorptive removal aptitude towards toxic Cr(VI) ions.
33966848	6	62	theme	maximum	777:783	arg1	capacity					796:803	a maximum adsorption capacity	775:803	a maximum adsorption capacity of 119.05 mg/g	775:818	The gained results refereed that a maximum adsorption capacity of 119.05 mg/g was attained by magnetic Fe3O4/ZIF-67@AmCs composite beads at 25 °C.
33966848	8	63	theme	aqueous	1164:1170	arg1	solutions					1172:1180	their aqueous solutions	1158:1180	their aqueous solutions	1158:1180	Besides, Fe3O4/ZIF-67@AmCs composite showed an excellent recyclability for the removal of Cr(VI) ions from their aqueous solutions for seven consecutive cycles.
33966848	2	64	theme	vast	286:289	arg1	obstacle					291:298	a vast obstacle	284:298	a vast obstacle which is their poor recyclability	284:332	However, MOFs have a vast obstacle which is their poor recyclability.
33966848	2	64	theme	vast	286:289	arg1	recyclability					320:332	their poor recyclability	309:332	their poor recyclability	309:332	However, MOFs have a vast obstacle which is their poor recyclability.
33966848	0	65	theme	aqueous	124:130	arg1	solutions					132:140	aqueous solutions	124:140	aqueous solutions	124:140	Facile fabrication of novel magnetic ZIF-67 MOF@aminated chitosan composite beads for the adsorptive removal of Cr(VI) from aqueous solutions.
33966848	8	66	from	solutions	1172:1180	arg1	removal					1130:1136	the removal	1126:1136	the removal of Cr(VI) ions from their aqueous solutions	1126:1180	Besides, Fe3O4/ZIF-67@AmCs composite showed an excellent recyclability for the removal of Cr(VI) ions from their aqueous solutions for seven consecutive cycles.
33966848	7	67	theme	Freundlich	929:938	arg1	models					949:954	Langmuir and Freundlich isotherm models	916:954	models	949:954	The process obeyed both of Langmuir and Freundlich isotherm models, and the pseudo 2nd order was more suitable kinetic model to represent the adsorption process.
33966848	3	68	theme	aminated	429:436	arg1	matrix					454:459	aminated chitosan (AmCs) matrix	429:459	aminated chitosan (AmCs) matrix to form core-dual shell Fe3O4/ZIF-67@AmCs composite beads	429:517	In this study, ZIF-67 was decorated with magnetic Fe3O4 nanoparticles, and then embedded into aminated chitosan (AmCs) matrix to form core-dual shell Fe3O4/ZIF-67@AmCs composite beads.
33966848	1	69	theme	premium	187:193	arg1	candidates					195:204	premium candidates	187:204	premium candidates for the removal of hazardous contaminants from wastewater	187:262	Metal organic frameworks (MOFs) have become premium candidates for the removal of hazardous contaminants from wastewater.
33966848	0	70	theme	chitosan	57:64	arg1	beads					76:80	novel magnetic ZIF-67 MOF@aminated chitosan composite beads	22:80	novel magnetic ZIF-67 MOF@aminated chitosan composite beads	22:80	Facile fabrication of novel magnetic ZIF-67 MOF@aminated chitosan composite beads for the adsorptive removal of Cr(VI) from aqueous solutions.
33966848	7	71	theme	isotherm	940:947	arg1	models					949:954	Langmuir and Freundlich isotherm models	916:954	models	949:954	The process obeyed both of Langmuir and Freundlich isotherm models, and the pseudo 2nd order was more suitable kinetic model to represent the adsorption process.
33966848	4	72	theme	analysis	528:535	arg1	tools					537:541	Diverse analysis tools	520:541	Diverse analysis tools	520:541	Diverse analysis tools were utilized to ensure the successful fabrication of the magnetic composite beads.
33966848	0	73	theme	aminated	48:55	arg1	beads					76:80	novel magnetic ZIF-67 MOF@aminated chitosan composite beads	22:80	novel magnetic ZIF-67 MOF@aminated chitosan composite beads	22:80	Facile fabrication of novel magnetic ZIF-67 MOF@aminated chitosan composite beads for the adsorptive removal of Cr(VI) from aqueous solutions.
33966848	7	74	theme	Langmuir	916:923	arg1	models					949:954	Langmuir and Freundlich isotherm models	916:954	models	949:954	The process obeyed both of Langmuir and Freundlich isotherm models, and the pseudo 2nd order was more suitable kinetic model to represent the adsorption process.
33966848	2	75	theme	poor	315:318	arg1	obstacle					291:298	a vast obstacle	284:298	a vast obstacle which is their poor recyclability	284:332	However, MOFs have a vast obstacle which is their poor recyclability.
33966848	2	75	theme	poor	315:318	arg1	recyclability					320:332	their poor recyclability	309:332	their poor recyclability	309:332	However, MOFs have a vast obstacle which is their poor recyclability.
34000312	6	0	theme	gut	886:888	arg1	microbiota					890:899	gut microbiota	886:899	gut microbiota	886:899	Moreover, BXS and CTBXS could change the composition and abundance of gut microbiota, could also promote the production of SCFAs.
34000312	1	1	theme	study	149:153	arg1	aim					137:139	The aim	133:139	The aim of this study	133:153	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	1	2	theme	starch	249:254	arg1	properties					221:230	the structural and physicochemical properties	186:230	the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS)	186:314	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	1	2	theme	starch	249:254	arg1	impacts					332:338	its related impacts	320:338	its related impacts	320:338	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	5	3	theme	higher	721:726	arg1	solubility					728:737	solubility	728:737	solubility	728:737	In addition, BXS exhibited significantly higher solubility and swelling power than CTBXS, and CTBXS had higher content of SDS than BXS.
34000312	1	4	theme	related	324:330	arg1	impacts					332:338	its related impacts	320:338	its related impacts	320:338	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	7	5	theme	in	994:995	arg1	digestion					1003:1011	the in vitro digestion and fecal fermentation behaviors	990:1044	digestion	1003:1011	This study is beneficial to well understand the in vitro digestion and fecal fermentation behaviors of BXS and CTBXS, and can be developed as a potential functional food with the aim of improving colonic health.
34000312	1	6	theme	gut	399:401	arg1	microbiota					403:412	human gut microbiota	393:412	human gut microbiota	393:412	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	7	7	theme	BXS	1049:1051	arg1	digestion					1003:1011	the in vitro digestion and fecal fermentation behaviors	990:1044	digestion	1003:1011	This study is beneficial to well understand the in vitro digestion and fecal fermentation behaviors of BXS and CTBXS, and can be developed as a potential functional food with the aim of improving colonic health.
34000312	7	7	theme	BXS	1049:1051	arg1	behaviors					1036:1044	the in vitro digestion and fecal fermentation behaviors	990:1044	behaviors	1036:1044	This study is beneficial to well understand the in vitro digestion and fecal fermentation behaviors of BXS and CTBXS, and can be developed as a potential functional food with the aim of improving colonic health.
34000312	6	8	dep	composition	857:867	arg1	the					853:855	the	853:855	the	853:855	Moreover, BXS and CTBXS could change the composition and abundance of gut microbiota, could also promote the production of SCFAs.
34000312	7	9	theme	potential	1090:1098	arg1	study					951:955	This study	946:955	This study	946:955	This study is beneficial to well understand the in vitro digestion and fecal fermentation behaviors of BXS and CTBXS, and can be developed as a potential functional food with the aim of improving colonic health.
34000312	7	9	theme	potential	1090:1098	arg1	food					1111:1114	a potential functional food	1088:1114	a potential functional food with the aim of improving colonic health	1088:1155	This study is beneficial to well understand the in vitro digestion and fecal fermentation behaviors of BXS and CTBXS, and can be developed as a potential functional food with the aim of improving colonic health.
34000312	7	10	theme	fecal	1017:1021	arg1	behaviors					1036:1044	the in vitro digestion and fecal fermentation behaviors	990:1044	behaviors	1036:1044	This study is beneficial to well understand the in vitro digestion and fecal fermentation behaviors of BXS and CTBXS, and can be developed as a potential functional food with the aim of improving colonic health.
34000312	1	11	theme	microbiota	403:412	arg1	production					343:352	production	343:352	production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation	343:443	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	3	12	contain	had	555:557	arg1	BXS					551:553	BXS	551:553	BXS	551:553	According to XRD and TGA, BXS had a C-type crystalline pattern, while CTBXS had a B-type crystalline pattern.
34000312	3	12	contain	had	555:557	arg2	pattern					580:586	a C-type crystalline pattern	559:586	a C-type crystalline pattern	559:586	According to XRD and TGA, BXS had a C-type crystalline pattern, while CTBXS had a B-type crystalline pattern.
34000312	7	13	theme	functional	1100:1109	arg1	study					951:955	This study	946:955	This study	946:955	This study is beneficial to well understand the in vitro digestion and fecal fermentation behaviors of BXS and CTBXS, and can be developed as a potential functional food with the aim of improving colonic health.
34000312	7	13	theme	functional	1100:1109	arg1	food					1111:1114	a potential functional food	1088:1114	a potential functional food with the aim of improving colonic health	1088:1155	This study is beneficial to well understand the in vitro digestion and fecal fermentation behaviors of BXS and CTBXS, and can be developed as a potential functional food with the aim of improving colonic health.
34000312	7	14	theme	fermentation	1023:1034	arg1	behaviors					1036:1044	the in vitro digestion and fecal fermentation behaviors	990:1044	behaviors	1036:1044	This study is beneficial to well understand the in vitro digestion and fecal fermentation behaviors of BXS and CTBXS, and can be developed as a potential functional food with the aim of improving colonic health.
34000312	4	15	theme	better	645:650	arg1	stability					660:668	better thermal stability	645:668	better thermal stability	645:668	CTBXS had better thermal stability than BXS.
34000312	0	16	theme	gut	117:119	arg1	microbiota					121:130	gut microbiota	117:130	gut microbiota	117:130	Preparation and characterization of native and autoclaving-cooling treated Pinellia ternate starch and its impact on gut microbiota.
34000312	1	17	theme	in	417:418	arg1	fermentation					432:443	in vitro fecal fermentation	417:443	in vitro fecal fermentation	417:443	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	2	18	theme	spherical	468:476	arg1	granules					478:485	spherical granules	468:485	spherical granules	468:485	BXS had semicircle to spherical granules, whereas CTBXS exhibited block-shape.
34000312	7	19	dep	in	994:995	arg1	vitro					997:1001	vitro	997:1001	vitro	997:1001	This study is beneficial to well understand the in vitro digestion and fecal fermentation behaviors of BXS and CTBXS, and can be developed as a potential functional food with the aim of improving colonic health.
34000312	5	20	theme	SDS	802:804	arg1	content					791:797	higher content	784:797	higher content of SDS	784:804	In addition, BXS exhibited significantly higher solubility and swelling power than CTBXS, and CTBXS had higher content of SDS than BXS.
34000312	1	21	theme	short	357:361	arg1	acids					375:379	short chain fatty acids	357:379	short chain fatty acids (SCFAs)	357:387	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	1	21	theme	short	357:361	arg1	SCFAs					382:386	SCFAs	382:386	SCFAs	382:386	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	5	22	dep	solubility	728:737	arg1	power					752:756	power	752:756	power	752:756	In addition, BXS exhibited significantly higher solubility and swelling power than CTBXS, and CTBXS had higher content of SDS than BXS.
34000312	1	23	theme	fecal	426:430	arg1	fermentation					432:443	in vitro fecal fermentation	417:443	in vitro fecal fermentation	417:443	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	3	24	theme	B-type	607:612	arg1	pattern					626:632	a B-type crystalline pattern	605:632	a B-type crystalline pattern	605:632	According to XRD and TGA, BXS had a C-type crystalline pattern, while CTBXS had a B-type crystalline pattern.
34000312	5	25	contain	had	780:782	arg1	CTBXS					774:778	CTBXS	774:778	CTBXS	774:778	In addition, BXS exhibited significantly higher solubility and swelling power than CTBXS, and CTBXS had higher content of SDS than BXS.
34000312	5	25	contain	had	780:782	arg2	content					791:797	higher content	784:797	higher content of SDS	784:804	In addition, BXS exhibited significantly higher solubility and swelling power than CTBXS, and CTBXS had higher content of SDS than BXS.
34000312	6	26	dep	change	846:851	arg1	promote					913:919	promote	913:919	could also promote the production of SCFAs	902:943	Moreover, BXS and CTBXS could change the composition and abundance of gut microbiota, could also promote the production of SCFAs.
34000312	1	27	theme	fatty	369:373	arg1	acids					375:379	short chain fatty acids	357:379	short chain fatty acids (SCFAs)	357:387	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	1	27	theme	fatty	369:373	arg1	SCFAs					382:386	SCFAs	382:386	SCFAs	382:386	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	5	28	theme	higher	784:789	arg1	content					791:797	higher content	784:797	higher content of SDS	784:804	In addition, BXS exhibited significantly higher solubility and swelling power than CTBXS, and CTBXS had higher content of SDS than BXS.
34000312	1	29	theme	autoclaving-cooling	266:284	arg1	CTBXS					309:313	CTBXS	309:313	CTBXS	309:313	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	1	29	theme	autoclaving-cooling	266:284	arg1	starch					301:306	autoclaving-cooling treated Banxia starch	266:306	autoclaving-cooling treated Banxia starch (CTBXS)	266:314	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	1	30	theme	treated	286:292	arg1	CTBXS					309:313	CTBXS	309:313	CTBXS	309:313	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	1	30	theme	treated	286:292	arg1	starch					301:306	autoclaving-cooling treated Banxia starch	266:306	autoclaving-cooling treated Banxia starch (CTBXS)	266:314	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	1	31	theme	chain	363:367	arg1	acids					375:379	short chain fatty acids	357:379	short chain fatty acids (SCFAs)	357:387	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	1	31	theme	chain	363:367	arg1	SCFAs					382:386	SCFAs	382:386	SCFAs	382:386	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	1	32	theme	structural	190:199	arg1	properties					221:230	the structural and physicochemical properties	186:230	the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS)	186:314	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	7	33	theme	CTBXS	1057:1061	arg1	digestion					1003:1011	the in vitro digestion and fecal fermentation behaviors	990:1044	digestion	1003:1011	This study is beneficial to well understand the in vitro digestion and fecal fermentation behaviors of BXS and CTBXS, and can be developed as a potential functional food with the aim of improving colonic health.
34000312	7	33	theme	CTBXS	1057:1061	arg1	behaviors					1036:1044	the in vitro digestion and fecal fermentation behaviors	990:1044	behaviors	1036:1044	This study is beneficial to well understand the in vitro digestion and fecal fermentation behaviors of BXS and CTBXS, and can be developed as a potential functional food with the aim of improving colonic health.
34000312	1	34	theme	Banxia	294:299	arg1	CTBXS					309:313	CTBXS	309:313	CTBXS	309:313	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	1	34	theme	Banxia	294:299	arg1	starch					301:306	autoclaving-cooling treated Banxia starch	266:306	autoclaving-cooling treated Banxia starch (CTBXS)	266:314	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	0	35	from	starch	92:97	arg1	microbiota					121:130	gut microbiota	117:130	gut microbiota	117:130	Preparation and characterization of native and autoclaving-cooling treated Pinellia ternate starch and its impact on gut microbiota.
34000312	3	36	contain	had	601:603	arg1	CTBXS					595:599	CTBXS	595:599	CTBXS	595:599	According to XRD and TGA, BXS had a C-type crystalline pattern, while CTBXS had a B-type crystalline pattern.
34000312	3	36	contain	had	601:603	arg2	pattern					626:632	a B-type crystalline pattern	605:632	a B-type crystalline pattern	605:632	According to XRD and TGA, BXS had a C-type crystalline pattern, while CTBXS had a B-type crystalline pattern.
34000312	0	37	theme	native	36:41	arg1	Pinellia					75:82	native and autoclaving-cooling treated Pinellia	36:82	native and autoclaving-cooling treated Pinellia	36:82	Preparation and characterization of native and autoclaving-cooling treated Pinellia ternate starch and its impact on gut microbiota.
34000312	1	38	theme	starch	301:306	arg1	properties					221:230	the structural and physicochemical properties	186:230	the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS)	186:314	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	1	38	theme	starch	301:306	arg1	impacts					332:338	its related impacts	320:338	its related impacts	320:338	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	1	39	theme	acids	375:379	arg1	production					343:352	production	343:352	production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation	343:443	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	1	40	theme	physicochemical	205:219	arg1	properties					221:230	the structural and physicochemical properties	186:230	the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS)	186:314	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	0	41	theme	treated	67:73	arg1	Pinellia					75:82	native and autoclaving-cooling treated Pinellia	36:82	native and autoclaving-cooling treated Pinellia	36:82	Preparation and characterization of native and autoclaving-cooling treated Pinellia ternate starch and its impact on gut microbiota.
34000312	6	42	theme	microbiota	890:899	arg1	abundance					873:881	abundance	873:881	abundance	873:881	Moreover, BXS and CTBXS could change the composition and abundance of gut microbiota, could also promote the production of SCFAs.
34000312	6	42	theme	microbiota	890:899	arg1	composition					857:867	composition	857:867	composition	857:867	Moreover, BXS and CTBXS could change the composition and abundance of gut microbiota, could also promote the production of SCFAs.
34000312	0	43	theme	autoclaving-cooling	47:65	arg1	Pinellia					75:82	native and autoclaving-cooling treated Pinellia	36:82	native and autoclaving-cooling treated Pinellia	36:82	Preparation and characterization of native and autoclaving-cooling treated Pinellia ternate starch and its impact on gut microbiota.
34000312	3	44	theme	crystalline	614:624	arg1	pattern					626:632	a B-type crystalline pattern	605:632	a B-type crystalline pattern	605:632	According to XRD and TGA, BXS had a C-type crystalline pattern, while CTBXS had a B-type crystalline pattern.
34000312	4	45	theme	thermal	652:658	arg1	stability					660:668	better thermal stability	645:668	better thermal stability	645:668	CTBXS had better thermal stability than BXS.
34000312	7	46	with	food	1111:1114	arg1	aim					1125:1127	the aim	1121:1127	the aim of improving colonic health	1121:1155	This study is beneficial to well understand the in vitro digestion and fecal fermentation behaviors of BXS and CTBXS, and can be developed as a potential functional food with the aim of improving colonic health.
34000312	3	47	theme	crystalline	568:578	arg1	pattern					580:586	a C-type crystalline pattern	559:586	a C-type crystalline pattern	559:586	According to XRD and TGA, BXS had a C-type crystalline pattern, while CTBXS had a B-type crystalline pattern.
34000312	1	48	dep	in	417:418	arg1	vitro					420:424	vitro	420:424	vitro	420:424	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	0	49	from	impact	107:112	arg1	microbiota					121:130	gut microbiota	117:130	gut microbiota	117:130	Preparation and characterization of native and autoclaving-cooling treated Pinellia ternate starch and its impact on gut microbiota.
34000312	6	50	theme	SCFAs	939:943	arg1	production					925:934	the production	921:934	the production of SCFAs	921:943	Moreover, BXS and CTBXS could change the composition and abundance of gut microbiota, could also promote the production of SCFAs.
34000312	1	51	theme	human	393:397	arg1	microbiota					403:412	human gut microbiota	393:412	human gut microbiota	393:412	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	7	52	theme	colonic	1142:1148	arg1	health					1150:1155	colonic health	1142:1155	colonic health	1142:1155	This study is beneficial to well understand the in vitro digestion and fecal fermentation behaviors of BXS and CTBXS, and can be developed as a potential functional food with the aim of improving colonic health.
34000312	1	53	theme	native	235:240	arg1	BXS					257:259	BXS	257:259	BXS	257:259	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	1	53	theme	native	235:240	arg1	starch					249:254	native Banxia starch	235:254	native Banxia starch (BXS)	235:260	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	0	54	theme	Pinellia	75:82	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of native and autoclaving-cooling treated Pinellia ternate starch and its impact on gut microbiota.
34000312	0	54	theme	Pinellia	75:82	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of native and autoclaving-cooling treated Pinellia ternate starch and its impact on gut microbiota.
34000312	4	55	contain	had	641:643	arg2	stability					660:668	better thermal stability	645:668	better thermal stability	645:668	CTBXS had better thermal stability than BXS.
34000312	4	55	contain	had	641:643	arg1	CTBXS					635:639	CTBXS	635:639	CTBXS	635:639	CTBXS had better thermal stability than BXS.
34000312	3	56	theme	C-type	561:566	arg1	pattern					580:586	a C-type crystalline pattern	559:586	a C-type crystalline pattern	559:586	According to XRD and TGA, BXS had a C-type crystalline pattern, while CTBXS had a B-type crystalline pattern.
34000312	1	57	theme	Banxia	242:247	arg1	BXS					257:259	BXS	257:259	BXS	257:259	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
34000312	1	57	theme	Banxia	242:247	arg1	starch					249:254	native Banxia starch	235:254	native Banxia starch (BXS)	235:260	The aim of this study was to investigate and compare the structural and physicochemical properties of native Banxia starch (BXS) and autoclaving-cooling treated Banxia starch (CTBXS) and its related impacts on production of short chain fatty acids (SCFAs) and human gut microbiota by in vitro fecal fermentation.
32084599	9	0	theme	anticancer	2327:2336	arg1	efficacies					2338:2347	better anticancer efficacies	2320:2347	better anticancer efficacies than single drug formulations or solution mixtures of dual drugs	2320:2412	Then we utilized the hydrogel for differential release of CDDP and IRN to achieve better synergistic efficacy of drugs in inhibiting the growth of cancer cell A549 and better anticancer efficacies than single drug formulations or solution mixtures of dual drugs in an A549-xenografted mouse model.
32084599	6	1	dep	drug-gelator	1490:1501	arg1	nanoparticle-gelator					1504:1523	nanoparticle-gelator	1504:1523	nanoparticle-gelator	1504:1523	We believe that the strategy of utilizing co-assembly of multiple pairs of entities (i.e. drug-gelator, nanoparticle-gelator) in composite materials provides a generalized method to design mechanically stable supramolecular hydrogels, and further promises an exact temporal control of drug dosing by packing individual drugs in co-assembled structures/domains to satisfy clinical demands from combination therapy.
32084599	6	1	dep	drug-gelator	1490:1501	arg1	i.e.					1485:1488	i.e.	1485:1488	i.e.	1485:1488	We believe that the strategy of utilizing co-assembly of multiple pairs of entities (i.e. drug-gelator, nanoparticle-gelator) in composite materials provides a generalized method to design mechanically stable supramolecular hydrogels, and further promises an exact temporal control of drug dosing by packing individual drugs in co-assembled structures/domains to satisfy clinical demands from combination therapy.
32084599	5	2	from	synergism	1259:1267	arg1	studies					1304:1310	cell inhibition studies	1288:1310	cell inhibition studies	1288:1310	Comparing to simple mixture of CDDP and IRN solution, CDDP/Pept-AlgNP/IRN hydrogel formulation demonstrated excelling synergism of CDDP and IRN in cell inhibition studies, with efficacious antitumor potency further proved in tumor regression studies in vivo.
32084599	4	3	theme	tunable	1044:1050	arg1	release					1052:1058	a tunable release	1042:1058	a tunable release of IRN that is dependent on fraction ratios of AlgNP in the composite materials	1042:1138	Next, the nanocomposite hydrogel with excellent injectability served as a depot for controlled release of dual drugs, and guaranteed a fast release of CDDP prior to a tunable release of IRN that is dependent on fraction ratios of AlgNP in the composite materials.
32084599	9	4	theme	differential	2186:2197	arg1	release					2199:2205	differential release	2186:2205	differential release of CDDP and IRN	2186:2221	Then we utilized the hydrogel for differential release of CDDP and IRN to achieve better synergistic efficacy of drugs in inhibiting the growth of cancer cell A549 and better anticancer efficacies than single drug formulations or solution mixtures of dual drugs in an A549-xenografted mouse model.
32084599	9	5	theme	A549-xenografted	2420:2435	arg1	model					2443:2447	an A549-xenografted mouse model	2417:2447	an A549-xenografted mouse model	2417:2447	Then we utilized the hydrogel for differential release of CDDP and IRN to achieve better synergistic efficacy of drugs in inhibiting the growth of cancer cell A549 and better anticancer efficacies than single drug formulations or solution mixtures of dual drugs in an A549-xenografted mouse model.
32084599	5	6	theme	solution	1185:1192	arg1	mixture					1161:1167	simple mixture	1154:1167	simple mixture of CDDP and IRN solution	1154:1192	Comparing to simple mixture of CDDP and IRN solution, CDDP/Pept-AlgNP/IRN hydrogel formulation demonstrated excelling synergism of CDDP and IRN in cell inhibition studies, with efficacious antitumor potency further proved in tumor regression studies in vivo.
32084599	0	7	link	Double-crosslinked	0:17	arg1	nanocomposite					19:31	Double-crosslinked nanocomposite	0:31	Double-crosslinked nanocomposite	0:31	Double-crosslinked nanocomposite hydrogels for temporal control of drug dosing in combination therapy.
32084599	9	8	theme	better	2320:2325	arg1	efficacies					2338:2347	better anticancer efficacies	2320:2347	better anticancer efficacies than single drug formulations or solution mixtures of dual drugs	2320:2412	Then we utilized the hydrogel for differential release of CDDP and IRN to achieve better synergistic efficacy of drugs in inhibiting the growth of cancer cell A549 and better anticancer efficacies than single drug formulations or solution mixtures of dual drugs in an A549-xenografted mouse model.
32084599	3	9	theme	double-crosslinking	508:526	arg1	strategy					528:535	a double-crosslinking strategy	506:535	a double-crosslinking strategy	506:535	First, a double-crosslinking strategy was exploited for structural reinforcement of hydrogel, with integration of coordination interactions between CDDP and hydrogelator (Pept) as well as electrostatic interactions between Pept and alginate nanoparticles (AlgNP/IRN), that afforded nanocomposite hydrogel with 42-fold increase in storage modulus comparing to peptide gel alone.
32084599	6	10	theme	composite	1529:1537	arg1	materials					1539:1547	composite materials	1529:1547	composite materials	1529:1547	We believe that the strategy of utilizing co-assembly of multiple pairs of entities (i.e. drug-gelator, nanoparticle-gelator) in composite materials provides a generalized method to design mechanically stable supramolecular hydrogels, and further promises an exact temporal control of drug dosing by packing individual drugs in co-assembled structures/domains to satisfy clinical demands from combination therapy.
32084599	9	11	used	utilized	2160:2167	arg2	we					2157:2158	we	2157:2158	we	2157:2158	Then we utilized the hydrogel for differential release of CDDP and IRN to achieve better synergistic efficacy of drugs in inhibiting the growth of cancer cell A549 and better anticancer efficacies than single drug formulations or solution mixtures of dual drugs in an A549-xenografted mouse model.
32084599	7	12	from	release	1996:2002	arg1	therapy					2047:2053	combination therapy	2035:2053	combination therapy	2035:2053	STATEMENT OF SIGNIFICANCE: This study reports the design of nanocomposite hydrogels with two distinct co-assembling domains for structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy.
32084599	6	13	theme	individual	1708:1717	arg1	drugs					1719:1723	individual drugs	1708:1723	individual drugs in co-assembled structures/domains	1708:1758	We believe that the strategy of utilizing co-assembly of multiple pairs of entities (i.e. drug-gelator, nanoparticle-gelator) in composite materials provides a generalized method to design mechanically stable supramolecular hydrogels, and further promises an exact temporal control of drug dosing by packing individual drugs in co-assembled structures/domains to satisfy clinical demands from combination therapy.
32084599	4	14	theme	fraction	1088:1095	arg1	ratios					1097:1102	fraction ratios	1088:1102	fraction ratios of AlgNP in the composite materials	1088:1138	Next, the nanocomposite hydrogel with excellent injectability served as a depot for controlled release of dual drugs, and guaranteed a fast release of CDDP prior to a tunable release of IRN that is dependent on fraction ratios of AlgNP in the composite materials.
32084599	9	15	theme	cell	2306:2309	arg1	growth					2289:2294	the growth	2285:2294	the growth of cancer cell A549 and better anticancer efficacies than single drug formulations or solution mixtures of dual drugs in an A549-xenografted mouse model	2285:2447	Then we utilized the hydrogel for differential release of CDDP and IRN to achieve better synergistic efficacy of drugs in inhibiting the growth of cancer cell A549 and better anticancer efficacies than single drug formulations or solution mixtures of dual drugs in an A549-xenografted mouse model.
32084599	9	16	theme	better	2234:2239	arg1	efficacy					2253:2260	better synergistic efficacy	2234:2260	better synergistic efficacy of drugs	2234:2269	Then we utilized the hydrogel for differential release of CDDP and IRN to achieve better synergistic efficacy of drugs in inhibiting the growth of cancer cell A549 and better anticancer efficacies than single drug formulations or solution mixtures of dual drugs in an A549-xenografted mouse model.
32084599	6	17	from	drugs	1719:1723	arg1	structures/domains					1741:1758	co-assembled structures/domains	1728:1758	co-assembled structures/domains	1728:1758	We believe that the strategy of utilizing co-assembly of multiple pairs of entities (i.e. drug-gelator, nanoparticle-gelator) in composite materials provides a generalized method to design mechanically stable supramolecular hydrogels, and further promises an exact temporal control of drug dosing by packing individual drugs in co-assembled structures/domains to satisfy clinical demands from combination therapy.
32084599	6	18	theme	supramolecular	1609:1622	arg1	hydrogels					1624:1632	mechanically stable supramolecular hydrogels	1589:1632	mechanically stable supramolecular hydrogels	1589:1632	We believe that the strategy of utilizing co-assembly of multiple pairs of entities (i.e. drug-gelator, nanoparticle-gelator) in composite materials provides a generalized method to design mechanically stable supramolecular hydrogels, and further promises an exact temporal control of drug dosing by packing individual drugs in co-assembled structures/domains to satisfy clinical demands from combination therapy.
32084599	6	19	from	therapy	1805:1811	arg1	demands					1780:1786	clinical demands	1771:1786	clinical demands from combination therapy	1771:1811	We believe that the strategy of utilizing co-assembly of multiple pairs of entities (i.e. drug-gelator, nanoparticle-gelator) in composite materials provides a generalized method to design mechanically stable supramolecular hydrogels, and further promises an exact temporal control of drug dosing by packing individual drugs in co-assembled structures/domains to satisfy clinical demands from combination therapy.
32084599	10	20	theme	drug	2605:2608	arg1	dosing					2610:2615	drug dosing	2605:2615	drug dosing	2605:2615	We believe that the strategy of packing individual drugs in distinct co-assembling structures promises a paradigm shift for regulating temporal control of drug dosing in combination therapy.
32084599	3	21	theme	hydrogel	583:590	arg1	reinforcement					566:578	structural reinforcement	555:578	structural reinforcement of hydrogel	555:590	First, a double-crosslinking strategy was exploited for structural reinforcement of hydrogel, with integration of coordination interactions between CDDP and hydrogelator (Pept) as well as electrostatic interactions between Pept and alginate nanoparticles (AlgNP/IRN), that afforded nanocomposite hydrogel with 42-fold increase in storage modulus comparing to peptide gel alone.
32084599	1	22	theme	dosing	128:133	arg1	control					112:118	Temporal control	103:118	Temporal control of drug dosing	103:133	Temporal control of drug dosing is indispensable for a successful combination therapy that utilizes cisplatin (CDDP) and irinotecan (IRN), with clinical evidence supporting a higher response rate when CDDP was administered prior to IRN.
32084599	4	23	theme	excellent	915:923	arg1	injectability					925:937	excellent injectability	915:937	excellent injectability	915:937	Next, the nanocomposite hydrogel with excellent injectability served as a depot for controlled release of dual drugs, and guaranteed a fast release of CDDP prior to a tunable release of IRN that is dependent on fraction ratios of AlgNP in the composite materials.
32084599	6	24	theme	drug	1685:1688	arg1	dosing					1690:1695	drug dosing	1685:1695	drug dosing	1685:1695	We believe that the strategy of utilizing co-assembly of multiple pairs of entities (i.e. drug-gelator, nanoparticle-gelator) in composite materials provides a generalized method to design mechanically stable supramolecular hydrogels, and further promises an exact temporal control of drug dosing by packing individual drugs in co-assembled structures/domains to satisfy clinical demands from combination therapy.
32084599	3	25	theme	42-fold	809:815	arg1	increase					817:824	42-fold increase	809:824	42-fold increase in storage modulus comparing to peptide gel alone	809:874	First, a double-crosslinking strategy was exploited for structural reinforcement of hydrogel, with integration of coordination interactions between CDDP and hydrogelator (Pept) as well as electrostatic interactions between Pept and alginate nanoparticles (AlgNP/IRN), that afforded nanocomposite hydrogel with 42-fold increase in storage modulus comparing to peptide gel alone.
32084599	3	26	theme	interactions	626:637	arg1	integration					598:608	integration	598:608	integration	598:608	First, a double-crosslinking strategy was exploited for structural reinforcement of hydrogel, with integration of coordination interactions between CDDP and hydrogelator (Pept) as well as electrostatic interactions between Pept and alginate nanoparticles (AlgNP/IRN), that afforded nanocomposite hydrogel with 42-fold increase in storage modulus comparing to peptide gel alone.
32084599	4	27	theme	dual	983:986	arg1	drugs					988:992	dual drugs	983:992	dual drugs	983:992	Next, the nanocomposite hydrogel with excellent injectability served as a depot for controlled release of dual drugs, and guaranteed a fast release of CDDP prior to a tunable release of IRN that is dependent on fraction ratios of AlgNP in the composite materials.
32084599	7	28	dep	STATEMENT	1814:1822	arg1	reports					1852:1858	reports	1852:1858	reports the design of nanocomposite hydrogels with two distinct co-assembling domains for structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy	1852:2053	STATEMENT OF SIGNIFICANCE: This study reports the design of nanocomposite hydrogels with two distinct co-assembling domains for structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy.
32084599	4	29	theme	composite	1120:1128	arg1	materials					1130:1138	the composite materials	1116:1138	the composite materials	1116:1138	Next, the nanocomposite hydrogel with excellent injectability served as a depot for controlled release of dual drugs, and guaranteed a fast release of CDDP prior to a tunable release of IRN that is dependent on fraction ratios of AlgNP in the composite materials.
32084599	1	30	theme	clinical	247:254	arg1	evidence					256:263	clinical evidence	247:263	clinical evidence supporting a higher response rate when CDDP was administered prior to IRN	247:337	Temporal control of drug dosing is indispensable for a successful combination therapy that utilizes cisplatin (CDDP) and irinotecan (IRN), with clinical evidence supporting a higher response rate when CDDP was administered prior to IRN.
32084599	6	31	theme	generalized	1560:1570	arg1	method					1572:1577	a generalized method	1558:1577	a generalized method to design mechanically stable supramolecular hydrogels, and further promises an exact temporal control of drug dosing by packing individual drugs in co-assembled structures/domains to satisfy clinical demands from combination therapy	1558:1811	We believe that the strategy of utilizing co-assembly of multiple pairs of entities (i.e. drug-gelator, nanoparticle-gelator) in composite materials provides a generalized method to design mechanically stable supramolecular hydrogels, and further promises an exact temporal control of drug dosing by packing individual drugs in co-assembled structures/domains to satisfy clinical demands from combination therapy.
32084599	9	32	theme	drugs	2265:2269	arg1	efficacy					2253:2260	better synergistic efficacy	2234:2260	better synergistic efficacy of drugs	2234:2269	Then we utilized the hydrogel for differential release of CDDP and IRN to achieve better synergistic efficacy of drugs in inhibiting the growth of cancer cell A549 and better anticancer efficacies than single drug formulations or solution mixtures of dual drugs in an A549-xenografted mouse model.
32084599	5	33	theme	excelling	1249:1257	arg1	synergism					1259:1267	excelling synergism	1249:1267	excelling synergism of CDDP and IRN in cell inhibition studies	1249:1310	Comparing to simple mixture of CDDP and IRN solution, CDDP/Pept-AlgNP/IRN hydrogel formulation demonstrated excelling synergism of CDDP and IRN in cell inhibition studies, with efficacious antitumor potency further proved in tumor regression studies in vivo.
32084599	0	34	theme	combination	82:92	arg1	therapy					94:100	combination therapy	82:100	combination therapy	82:100	Double-crosslinked nanocomposite hydrogels for temporal control of drug dosing in combination therapy.
32084599	6	35	theme	temporal	1665:1672	arg1	control					1674:1680	an exact temporal control	1656:1680	an exact temporal control of drug dosing	1656:1695	We believe that the strategy of utilizing co-assembly of multiple pairs of entities (i.e. drug-gelator, nanoparticle-gelator) in composite materials provides a generalized method to design mechanically stable supramolecular hydrogels, and further promises an exact temporal control of drug dosing by packing individual drugs in co-assembled structures/domains to satisfy clinical demands from combination therapy.
32084599	1	36	theme	combination	169:179	arg1	therapy					181:187	a successful combination therapy	156:187	a successful combination therapy that utilizes cisplatin (CDDP) and irinotecan (IRN), with clinical evidence supporting a higher response rate when CDDP was administered prior to IRN	156:337	Temporal control of drug dosing is indispensable for a successful combination therapy that utilizes cisplatin (CDDP) and irinotecan (IRN), with clinical evidence supporting a higher response rate when CDDP was administered prior to IRN.
32084599	9	37	theme	cancer	2299:2304	arg1	cell					2306:2309	cancer cell A549 and better anticancer efficacies than single drug formulations or solution mixtures of dual drugs	2299:2412	cell	2306:2309	Then we utilized the hydrogel for differential release of CDDP and IRN to achieve better synergistic efficacy of drugs in inhibiting the growth of cancer cell A549 and better anticancer efficacies than single drug formulations or solution mixtures of dual drugs in an A549-xenografted mouse model.
32084599	7	38	dep	drugs	2011:2015	arg1	IRN					2027:2029	IRN	2027:2029	IRN	2027:2029	STATEMENT OF SIGNIFICANCE: This study reports the design of nanocomposite hydrogels with two distinct co-assembling domains for structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy.
32084599	7	38	dep	drugs	2011:2015	arg1	CDDP					2018:2021	CDDP	2018:2021	CDDP	2018:2021	STATEMENT OF SIGNIFICANCE: This study reports the design of nanocomposite hydrogels with two distinct co-assembling domains for structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy.
32084599	7	38	dep	drugs	2011:2015	arg1	drugs					2011:2015	two drugs	2007:2015	two drugs (CDDP and IRN)	2007:2030	STATEMENT OF SIGNIFICANCE: This study reports the design of nanocomposite hydrogels with two distinct co-assembling domains for structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy.
32084599	5	39	theme	efficacious	1318:1328	arg1	potency					1340:1346	efficacious antitumor potency further	1318:1354	efficacious antitumor potency further proved in tumor regression studies in vivo	1318:1397	Comparing to simple mixture of CDDP and IRN solution, CDDP/Pept-AlgNP/IRN hydrogel formulation demonstrated excelling synergism of CDDP and IRN in cell inhibition studies, with efficacious antitumor potency further proved in tumor regression studies in vivo.
32084599	7	40	theme	release	1996:2002	arg1	reinforcement					1953:1965	structural reinforcement	1942:1965	structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy	1942:2053	STATEMENT OF SIGNIFICANCE: This study reports the design of nanocomposite hydrogels with two distinct co-assembling domains for structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy.
32084599	10	41	theme	packing	2482:2488	arg1	drugs					2501:2505	packing individual drugs	2482:2505	packing individual drugs in distinct co-assembling structures	2482:2542	We believe that the strategy of packing individual drugs in distinct co-assembling structures promises a paradigm shift for regulating temporal control of drug dosing in combination therapy.
32084599	2	42	theme	nanocomposite	364:376	arg1	hydrogel					378:385	a peptide-based nanocomposite hydrogel	348:385	a peptide-based nanocomposite hydrogel (CDDP/Pept-AlgNP/IRN)	348:407	Herein, a peptide-based nanocomposite hydrogel (CDDP/Pept-AlgNP/IRN) was designed for differential release of CDDP and IRN to maximize synergism of two drugs.
32084599	2	42	theme	nanocomposite	364:376	arg1	CDDP/Pept-AlgNP/IRN					388:406	CDDP/Pept-AlgNP/IRN	388:406	CDDP/Pept-AlgNP/IRN	388:406	Herein, a peptide-based nanocomposite hydrogel (CDDP/Pept-AlgNP/IRN) was designed for differential release of CDDP and IRN to maximize synergism of two drugs.
32084599	0	43	theme	Double-crosslinked	0:17	arg1	nanocomposite					19:31	Double-crosslinked nanocomposite	0:31	Double-crosslinked nanocomposite	0:31	Double-crosslinked nanocomposite hydrogels for temporal control of drug dosing in combination therapy.
32084599	1	44	theme	Temporal	103:110	arg1	control					112:118	Temporal control	103:118	Temporal control of drug dosing	103:133	Temporal control of drug dosing is indispensable for a successful combination therapy that utilizes cisplatin (CDDP) and irinotecan (IRN), with clinical evidence supporting a higher response rate when CDDP was administered prior to IRN.
32084599	2	45	theme	differential	426:437	arg1	release					439:445	differential release	426:445	differential release of CDDP and IRN	426:461	Herein, a peptide-based nanocomposite hydrogel (CDDP/Pept-AlgNP/IRN) was designed for differential release of CDDP and IRN to maximize synergism of two drugs.
32084599	7	46	theme	SIGNIFICANCE	1827:1838	arg1	STATEMENT					1814:1822	STATEMENT	1814:1822	STATEMENT OF SIGNIFICANCE: This study reports the design of nanocomposite hydrogels with two distinct co-assembling domains for structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy.	1814:2054	STATEMENT OF SIGNIFICANCE: This study reports the design of nanocomposite hydrogels with two distinct co-assembling domains for structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy.
32084599	5	47	theme	inhibition	1293:1302	arg1	studies					1304:1310	cell inhibition studies	1288:1310	cell inhibition studies	1288:1310	Comparing to simple mixture of CDDP and IRN solution, CDDP/Pept-AlgNP/IRN hydrogel formulation demonstrated excelling synergism of CDDP and IRN in cell inhibition studies, with efficacious antitumor potency further proved in tumor regression studies in vivo.
32084599	9	48	theme	efficacies	2338:2347	arg1	growth					2289:2294	the growth	2285:2294	the growth of cancer cell A549 and better anticancer efficacies than single drug formulations or solution mixtures of dual drugs in an A549-xenografted mouse model	2285:2447	Then we utilized the hydrogel for differential release of CDDP and IRN to achieve better synergistic efficacy of drugs in inhibiting the growth of cancer cell A549 and better anticancer efficacies than single drug formulations or solution mixtures of dual drugs in an A549-xenografted mouse model.
32084599	1	49	theme	higher	278:283	arg1	rate					294:297	a higher response rate	276:297	a higher response rate when CDDP was administered prior to IRN	276:337	Temporal control of drug dosing is indispensable for a successful combination therapy that utilizes cisplatin (CDDP) and irinotecan (IRN), with clinical evidence supporting a higher response rate when CDDP was administered prior to IRN.
32084599	4	50	with	hydrogel	901:908	arg1	injectability					925:937	excellent injectability	915:937	excellent injectability	915:937	Next, the nanocomposite hydrogel with excellent injectability served as a depot for controlled release of dual drugs, and guaranteed a fast release of CDDP prior to a tunable release of IRN that is dependent on fraction ratios of AlgNP in the composite materials.
32084599	8	51	theme	hydrogel	2142:2149	arg1	reinforcement					2125:2137	the reinforcement	2121:2137	the reinforcement of hydrogel	2121:2149	We first investigated the effects of co-assembling processes for the reinforcement of hydrogel.
32084599	10	52	theme	distinct	2510:2517	arg1	structures					2533:2542	distinct co-assembling structures	2510:2542	distinct co-assembling structures	2510:2542	We believe that the strategy of packing individual drugs in distinct co-assembling structures promises a paradigm shift for regulating temporal control of drug dosing in combination therapy.
32084599	3	53	theme	peptide	858:864	arg1	gel					866:868	peptide gel	858:868	peptide gel alone	858:874	First, a double-crosslinking strategy was exploited for structural reinforcement of hydrogel, with integration of coordination interactions between CDDP and hydrogelator (Pept) as well as electrostatic interactions between Pept and alginate nanoparticles (AlgNP/IRN), that afforded nanocomposite hydrogel with 42-fold increase in storage modulus comparing to peptide gel alone.
32084599	9	54	theme	single	2354:2359	arg1	formulations					2366:2377	single drug formulations	2354:2377	single drug formulations	2354:2377	Then we utilized the hydrogel for differential release of CDDP and IRN to achieve better synergistic efficacy of drugs in inhibiting the growth of cancer cell A549 and better anticancer efficacies than single drug formulations or solution mixtures of dual drugs in an A549-xenografted mouse model.
32084599	0	55	theme	temporal	47:54	arg1	control					56:62	temporal control	47:62	temporal control of drug dosing in combination therapy	47:100	Double-crosslinked nanocomposite hydrogels for temporal control of drug dosing in combination therapy.
32084599	7	56	theme	structural	1942:1951	arg1	reinforcement					1953:1965	structural reinforcement	1942:1965	structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy	1942:2053	STATEMENT OF SIGNIFICANCE: This study reports the design of nanocomposite hydrogels with two distinct co-assembling domains for structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy.
32084599	7	57	theme	hydrogels	1888:1896	arg1	design					1864:1869	the design	1860:1869	the design of nanocomposite hydrogels with two distinct co-assembling domains for structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy	1860:2053	STATEMENT OF SIGNIFICANCE: This study reports the design of nanocomposite hydrogels with two distinct co-assembling domains for structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy.
32084599	4	58	theme	fast	1012:1015	arg1	depot					951:955	a depot	949:955	a depot for controlled release of dual drugs	949:992	Next, the nanocomposite hydrogel with excellent injectability served as a depot for controlled release of dual drugs, and guaranteed a fast release of CDDP prior to a tunable release of IRN that is dependent on fraction ratios of AlgNP in the composite materials.
32084599	4	58	theme	fast	1012:1015	arg1	hydrogel					901:908	the nanocomposite hydrogel	883:908	the nanocomposite hydrogel with excellent injectability	883:937	Next, the nanocomposite hydrogel with excellent injectability served as a depot for controlled release of dual drugs, and guaranteed a fast release of CDDP prior to a tunable release of IRN that is dependent on fraction ratios of AlgNP in the composite materials.
32084599	4	58	theme	fast	1012:1015	arg1	release					1017:1023	a fast release	1010:1023	a fast release of CDDP	1010:1031	Next, the nanocomposite hydrogel with excellent injectability served as a depot for controlled release of dual drugs, and guaranteed a fast release of CDDP prior to a tunable release of IRN that is dependent on fraction ratios of AlgNP in the composite materials.
32084599	0	59	theme	dosing	72:77	arg1	control					56:62	temporal control	47:62	temporal control of drug dosing in combination therapy	47:100	Double-crosslinked nanocomposite hydrogels for temporal control of drug dosing in combination therapy.
32084599	8	60	theme	co-assembling	2093:2105	arg1	processes					2107:2115	co-assembling processes	2093:2115	co-assembling processes	2093:2115	We first investigated the effects of co-assembling processes for the reinforcement of hydrogel.
32084599	4	61	theme	IRN	1063:1065	arg1	release					1052:1058	a tunable release	1042:1058	a tunable release of IRN that is dependent on fraction ratios of AlgNP in the composite materials	1042:1138	Next, the nanocomposite hydrogel with excellent injectability served as a depot for controlled release of dual drugs, and guaranteed a fast release of CDDP prior to a tunable release of IRN that is dependent on fraction ratios of AlgNP in the composite materials.
32084599	6	62	theme	combination	1793:1803	arg1	therapy					1805:1811	combination therapy	1793:1811	combination therapy	1793:1811	We believe that the strategy of utilizing co-assembly of multiple pairs of entities (i.e. drug-gelator, nanoparticle-gelator) in composite materials provides a generalized method to design mechanically stable supramolecular hydrogels, and further promises an exact temporal control of drug dosing by packing individual drugs in co-assembled structures/domains to satisfy clinical demands from combination therapy.
32084599	9	63	theme	solution	2382:2389	arg1	mixtures					2391:2398	solution mixtures	2382:2398	solution mixtures	2382:2398	Then we utilized the hydrogel for differential release of CDDP and IRN to achieve better synergistic efficacy of drugs in inhibiting the growth of cancer cell A549 and better anticancer efficacies than single drug formulations or solution mixtures of dual drugs in an A549-xenografted mouse model.
32084599	7	64	theme	distinct	1907:1914	arg1	domains					1930:1936	two distinct co-assembling domains	1903:1936	two distinct co-assembling domains for structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy	1903:2053	STATEMENT OF SIGNIFICANCE: This study reports the design of nanocomposite hydrogels with two distinct co-assembling domains for structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy.
32084599	6	65	from	co-assembly	1442:1452	arg1	materials					1539:1547	composite materials	1529:1547	composite materials	1529:1547	We believe that the strategy of utilizing co-assembly of multiple pairs of entities (i.e. drug-gelator, nanoparticle-gelator) in composite materials provides a generalized method to design mechanically stable supramolecular hydrogels, and further promises an exact temporal control of drug dosing by packing individual drugs in co-assembled structures/domains to satisfy clinical demands from combination therapy.
32084599	9	66	theme	drug	2361:2364	arg1	formulations					2366:2377	single drug formulations	2354:2377	single drug formulations	2354:2377	Then we utilized the hydrogel for differential release of CDDP and IRN to achieve better synergistic efficacy of drugs in inhibiting the growth of cancer cell A549 and better anticancer efficacies than single drug formulations or solution mixtures of dual drugs in an A549-xenografted mouse model.
32084599	5	67	theme	hydrogel	1215:1222	arg1	formulation					1224:1234	CDDP/Pept-AlgNP/IRN hydrogel formulation	1195:1234	CDDP/Pept-AlgNP/IRN hydrogel formulation	1195:1234	Comparing to simple mixture of CDDP and IRN solution, CDDP/Pept-AlgNP/IRN hydrogel formulation demonstrated excelling synergism of CDDP and IRN in cell inhibition studies, with efficacious antitumor potency further proved in tumor regression studies in vivo.
32084599	8	68	theme	processes	2107:2115	arg1	effects					2082:2088	the effects	2078:2088	the effects of co-assembling processes for the reinforcement of hydrogel	2078:2149	We first investigated the effects of co-assembling processes for the reinforcement of hydrogel.
32084599	7	69	with	design	1864:1869	arg1	domains					1930:1936	two distinct co-assembling domains	1903:1936	two distinct co-assembling domains for structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy	1903:2053	STATEMENT OF SIGNIFICANCE: This study reports the design of nanocomposite hydrogels with two distinct co-assembling domains for structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy.
32084599	3	70	theme	interactions	701:712	arg1	integration					598:608	integration	598:608	integration	598:608	First, a double-crosslinking strategy was exploited for structural reinforcement of hydrogel, with integration of coordination interactions between CDDP and hydrogelator (Pept) as well as electrostatic interactions between Pept and alginate nanoparticles (AlgNP/IRN), that afforded nanocomposite hydrogel with 42-fold increase in storage modulus comparing to peptide gel alone.
32084599	5	71	theme	tumor	1366:1370	arg1	studies					1383:1389	tumor regression studies	1366:1389	tumor regression studies	1366:1389	Comparing to simple mixture of CDDP and IRN solution, CDDP/Pept-AlgNP/IRN hydrogel formulation demonstrated excelling synergism of CDDP and IRN in cell inhibition studies, with efficacious antitumor potency further proved in tumor regression studies in vivo.
32084599	9	72	theme	drugs	2408:2412	arg1	formulations					2366:2377	single drug formulations	2354:2377	single drug formulations	2354:2377	Then we utilized the hydrogel for differential release of CDDP and IRN to achieve better synergistic efficacy of drugs in inhibiting the growth of cancer cell A549 and better anticancer efficacies than single drug formulations or solution mixtures of dual drugs in an A549-xenografted mouse model.
32084599	9	72	theme	drugs	2408:2412	arg1	mixtures					2391:2398	solution mixtures	2382:2398	solution mixtures	2382:2398	Then we utilized the hydrogel for differential release of CDDP and IRN to achieve better synergistic efficacy of drugs in inhibiting the growth of cancer cell A549 and better anticancer efficacies than single drug formulations or solution mixtures of dual drugs in an A549-xenografted mouse model.
32084599	6	73	theme	multiple	1457:1464	arg1	pairs					1466:1470	multiple pairs	1457:1470	multiple pairs of entities (i.e. drug-gelator, nanoparticle-gelator)	1457:1524	We believe that the strategy of utilizing co-assembly of multiple pairs of entities (i.e. drug-gelator, nanoparticle-gelator) in composite materials provides a generalized method to design mechanically stable supramolecular hydrogels, and further promises an exact temporal control of drug dosing by packing individual drugs in co-assembled structures/domains to satisfy clinical demands from combination therapy.
32084599	5	74	theme	CDDP	1172:1175	arg1	mixture					1161:1167	simple mixture	1154:1167	simple mixture of CDDP and IRN solution	1154:1192	Comparing to simple mixture of CDDP and IRN solution, CDDP/Pept-AlgNP/IRN hydrogel formulation demonstrated excelling synergism of CDDP and IRN in cell inhibition studies, with efficacious antitumor potency further proved in tumor regression studies in vivo.
32084599	5	75	theme	IRN	1181:1183	arg1	solution					1185:1192	IRN solution	1181:1192	IRN solution	1181:1192	Comparing to simple mixture of CDDP and IRN solution, CDDP/Pept-AlgNP/IRN hydrogel formulation demonstrated excelling synergism of CDDP and IRN in cell inhibition studies, with efficacious antitumor potency further proved in tumor regression studies in vivo.
32084599	4	76	theme	AlgNP	1107:1111	arg1	ratios					1097:1102	fraction ratios	1088:1102	fraction ratios of AlgNP in the composite materials	1088:1138	Next, the nanocomposite hydrogel with excellent injectability served as a depot for controlled release of dual drugs, and guaranteed a fast release of CDDP prior to a tunable release of IRN that is dependent on fraction ratios of AlgNP in the composite materials.
32084599	3	77	theme	alginate	731:738	arg1	AlgNP/IRN					755:763	AlgNP/IRN	755:763	AlgNP/IRN	755:763	First, a double-crosslinking strategy was exploited for structural reinforcement of hydrogel, with integration of coordination interactions between CDDP and hydrogelator (Pept) as well as electrostatic interactions between Pept and alginate nanoparticles (AlgNP/IRN), that afforded nanocomposite hydrogel with 42-fold increase in storage modulus comparing to peptide gel alone.
32084599	3	77	theme	alginate	731:738	arg1	nanoparticles					740:752	alginate nanoparticles	731:752	alginate nanoparticles (AlgNP/IRN)	731:764	First, a double-crosslinking strategy was exploited for structural reinforcement of hydrogel, with integration of coordination interactions between CDDP and hydrogelator (Pept) as well as electrostatic interactions between Pept and alginate nanoparticles (AlgNP/IRN), that afforded nanocomposite hydrogel with 42-fold increase in storage modulus comparing to peptide gel alone.
32084599	9	78	theme	mouse	2437:2441	arg1	model					2443:2447	an A549-xenografted mouse model	2417:2447	an A549-xenografted mouse model	2417:2447	Then we utilized the hydrogel for differential release of CDDP and IRN to achieve better synergistic efficacy of drugs in inhibiting the growth of cancer cell A549 and better anticancer efficacies than single drug formulations or solution mixtures of dual drugs in an A549-xenografted mouse model.
32084599	10	79	theme	individual	2490:2499	arg1	drugs					2501:2505	packing individual drugs	2482:2505	packing individual drugs in distinct co-assembling structures	2482:2542	We believe that the strategy of packing individual drugs in distinct co-assembling structures promises a paradigm shift for regulating temporal control of drug dosing in combination therapy.
32084599	7	80	from	therapy	2047:2053	arg1	reinforcement					1953:1965	structural reinforcement	1942:1965	structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy	1942:2053	STATEMENT OF SIGNIFICANCE: This study reports the design of nanocomposite hydrogels with two distinct co-assembling domains for structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy.
32084599	9	81	theme	CDDP	2210:2213	arg1	release					2199:2205	differential release	2186:2205	differential release of CDDP and IRN	2186:2221	Then we utilized the hydrogel for differential release of CDDP and IRN to achieve better synergistic efficacy of drugs in inhibiting the growth of cancer cell A549 and better anticancer efficacies than single drug formulations or solution mixtures of dual drugs in an A549-xenografted mouse model.
32084599	3	82	theme	structural	555:564	arg1	reinforcement					566:578	structural reinforcement	555:578	structural reinforcement of hydrogel	555:590	First, a double-crosslinking strategy was exploited for structural reinforcement of hydrogel, with integration of coordination interactions between CDDP and hydrogelator (Pept) as well as electrostatic interactions between Pept and alginate nanoparticles (AlgNP/IRN), that afforded nanocomposite hydrogel with 42-fold increase in storage modulus comparing to peptide gel alone.
32084599	4	83	from	AlgNP	1107:1111	arg1	materials					1130:1138	the composite materials	1116:1138	the composite materials	1116:1138	Next, the nanocomposite hydrogel with excellent injectability served as a depot for controlled release of dual drugs, and guaranteed a fast release of CDDP prior to a tunable release of IRN that is dependent on fraction ratios of AlgNP in the composite materials.
32084599	7	84	from	hydrogel	1970:1977	arg1	therapy					2047:2053	combination therapy	2035:2053	combination therapy	2035:2053	STATEMENT OF SIGNIFICANCE: This study reports the design of nanocomposite hydrogels with two distinct co-assembling domains for structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy.
32084599	10	85	theme	co-assembling	2519:2531	arg1	structures					2533:2542	distinct co-assembling structures	2510:2542	distinct co-assembling structures	2510:2542	We believe that the strategy of packing individual drugs in distinct co-assembling structures promises a paradigm shift for regulating temporal control of drug dosing in combination therapy.
32084599	9	86	theme	IRN	2219:2221	arg1	release					2199:2205	differential release	2186:2205	differential release of CDDP and IRN	2186:2221	Then we utilized the hydrogel for differential release of CDDP and IRN to achieve better synergistic efficacy of drugs in inhibiting the growth of cancer cell A549 and better anticancer efficacies than single drug formulations or solution mixtures of dual drugs in an A549-xenografted mouse model.
32084599	4	87	from	ratios	1097:1102	arg1	materials					1130:1138	the composite materials	1116:1138	the composite materials	1116:1138	Next, the nanocomposite hydrogel with excellent injectability served as a depot for controlled release of dual drugs, and guaranteed a fast release of CDDP prior to a tunable release of IRN that is dependent on fraction ratios of AlgNP in the composite materials.
32084599	7	88	theme	combination	2035:2045	arg1	therapy					2047:2053	combination therapy	2035:2053	combination therapy	2035:2053	STATEMENT OF SIGNIFICANCE: This study reports the design of nanocomposite hydrogels with two distinct co-assembling domains for structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy.
32084599	9	89	from	growth	2289:2294	arg1	model					2443:2447	an A549-xenografted mouse model	2417:2447	an A549-xenografted mouse model	2417:2447	Then we utilized the hydrogel for differential release of CDDP and IRN to achieve better synergistic efficacy of drugs in inhibiting the growth of cancer cell A549 and better anticancer efficacies than single drug formulations or solution mixtures of dual drugs in an A549-xenografted mouse model.
32084599	10	90	from	structures	2533:2542	arg1	strategy					2470:2477	the strategy	2466:2477	the strategy of packing individual drugs in distinct co-assembling structures	2466:2542	We believe that the strategy of packing individual drugs in distinct co-assembling structures promises a paradigm shift for regulating temporal control of drug dosing in combination therapy.
32084599	10	91	theme	paradigm	2555:2562	arg1	shift					2564:2568	a paradigm shift	2553:2568	a paradigm shift for regulating temporal control of drug dosing in combination therapy	2553:2638	We believe that the strategy of packing individual drugs in distinct co-assembling structures promises a paradigm shift for regulating temporal control of drug dosing in combination therapy.
32084599	3	92	theme	nanocomposite	781:793	arg1	hydrogel					795:802	nanocomposite hydrogel	781:802	nanocomposite hydrogel	781:802	First, a double-crosslinking strategy was exploited for structural reinforcement of hydrogel, with integration of coordination interactions between CDDP and hydrogelator (Pept) as well as electrostatic interactions between Pept and alginate nanoparticles (AlgNP/IRN), that afforded nanocomposite hydrogel with 42-fold increase in storage modulus comparing to peptide gel alone.
32084599	6	93	theme	stable	1602:1607	arg1	hydrogels					1624:1632	mechanically stable supramolecular hydrogels	1589:1632	mechanically stable supramolecular hydrogels	1589:1632	We believe that the strategy of utilizing co-assembly of multiple pairs of entities (i.e. drug-gelator, nanoparticle-gelator) in composite materials provides a generalized method to design mechanically stable supramolecular hydrogels, and further promises an exact temporal control of drug dosing by packing individual drugs in co-assembled structures/domains to satisfy clinical demands from combination therapy.
32084599	1	94	theme	drug	123:126	arg1	dosing					128:133	drug dosing	123:133	drug dosing	123:133	Temporal control of drug dosing is indispensable for a successful combination therapy that utilizes cisplatin (CDDP) and irinotecan (IRN), with clinical evidence supporting a higher response rate when CDDP was administered prior to IRN.
32084599	4	95	theme	nanocomposite	887:899	arg1	depot					951:955	a depot	949:955	a depot for controlled release of dual drugs	949:992	Next, the nanocomposite hydrogel with excellent injectability served as a depot for controlled release of dual drugs, and guaranteed a fast release of CDDP prior to a tunable release of IRN that is dependent on fraction ratios of AlgNP in the composite materials.
32084599	4	95	theme	nanocomposite	887:899	arg1	hydrogel					901:908	the nanocomposite hydrogel	883:908	the nanocomposite hydrogel with excellent injectability	883:937	Next, the nanocomposite hydrogel with excellent injectability served as a depot for controlled release of dual drugs, and guaranteed a fast release of CDDP prior to a tunable release of IRN that is dependent on fraction ratios of AlgNP in the composite materials.
32084599	4	95	theme	nanocomposite	887:899	arg1	release					1017:1023	a fast release	1010:1023	a fast release of CDDP	1010:1031	Next, the nanocomposite hydrogel with excellent injectability served as a depot for controlled release of dual drugs, and guaranteed a fast release of CDDP prior to a tunable release of IRN that is dependent on fraction ratios of AlgNP in the composite materials.
32084599	6	96	theme	dosing	1690:1695	arg1	control					1674:1680	an exact temporal control	1656:1680	an exact temporal control of drug dosing	1656:1695	We believe that the strategy of utilizing co-assembly of multiple pairs of entities (i.e. drug-gelator, nanoparticle-gelator) in composite materials provides a generalized method to design mechanically stable supramolecular hydrogels, and further promises an exact temporal control of drug dosing by packing individual drugs in co-assembled structures/domains to satisfy clinical demands from combination therapy.
32084599	5	97	theme	CDDP	1272:1275	arg1	synergism					1259:1267	excelling synergism	1249:1267	excelling synergism of CDDP and IRN in cell inhibition studies	1249:1310	Comparing to simple mixture of CDDP and IRN solution, CDDP/Pept-AlgNP/IRN hydrogel formulation demonstrated excelling synergism of CDDP and IRN in cell inhibition studies, with efficacious antitumor potency further proved in tumor regression studies in vivo.
32084599	3	98	theme	coordination	613:624	arg1	interactions					626:637	coordination interactions	613:637	coordination interactions between CDDP and hydrogelator (Pept) as well as electrostatic interactions between Pept and alginate nanoparticles (AlgNP/IRN)	613:764	First, a double-crosslinking strategy was exploited for structural reinforcement of hydrogel, with integration of coordination interactions between CDDP and hydrogelator (Pept) as well as electrostatic interactions between Pept and alginate nanoparticles (AlgNP/IRN), that afforded nanocomposite hydrogel with 42-fold increase in storage modulus comparing to peptide gel alone.
32084599	10	99	theme	temporal	2585:2592	arg1	control					2594:2600	temporal control	2585:2600	temporal control of drug dosing	2585:2615	We believe that the strategy of packing individual drugs in distinct co-assembling structures promises a paradigm shift for regulating temporal control of drug dosing in combination therapy.
32084599	5	100	theme	IRN	1281:1283	arg1	synergism					1259:1267	excelling synergism	1249:1267	excelling synergism of CDDP and IRN in cell inhibition studies	1249:1310	Comparing to simple mixture of CDDP and IRN solution, CDDP/Pept-AlgNP/IRN hydrogel formulation demonstrated excelling synergism of CDDP and IRN in cell inhibition studies, with efficacious antitumor potency further proved in tumor regression studies in vivo.
32084599	6	101	dep	method	1572:1577	arg1	design					1582:1587	design	1582:1587	to design mechanically stable supramolecular hydrogels	1579:1632	We believe that the strategy of utilizing co-assembly of multiple pairs of entities (i.e. drug-gelator, nanoparticle-gelator) in composite materials provides a generalized method to design mechanically stable supramolecular hydrogels, and further promises an exact temporal control of drug dosing by packing individual drugs in co-assembled structures/domains to satisfy clinical demands from combination therapy.
32084599	6	101	dep	method	1572:1577	arg1	promises					1647:1654	promises	1647:1654	promises an exact temporal control of drug dosing by packing individual drugs in co-assembled structures/domains to satisfy clinical demands from combination therapy	1647:1811	We believe that the strategy of utilizing co-assembly of multiple pairs of entities (i.e. drug-gelator, nanoparticle-gelator) in composite materials provides a generalized method to design mechanically stable supramolecular hydrogels, and further promises an exact temporal control of drug dosing by packing individual drugs in co-assembled structures/domains to satisfy clinical demands from combination therapy.
32084599	10	102	from	strategy	2470:2477	arg1	structures					2533:2542	distinct co-assembling structures	2510:2542	distinct co-assembling structures	2510:2542	We believe that the strategy of packing individual drugs in distinct co-assembling structures promises a paradigm shift for regulating temporal control of drug dosing in combination therapy.
32084599	10	103	theme	combination	2620:2630	arg1	therapy					2632:2638	combination therapy	2620:2638	combination therapy	2620:2638	We believe that the strategy of packing individual drugs in distinct co-assembling structures promises a paradigm shift for regulating temporal control of drug dosing in combination therapy.
32084599	7	104	theme	drugs	2011:2015	arg1	hydrogel					1970:1977	hydrogel	1970:1977	hydrogel	1970:1977	STATEMENT OF SIGNIFICANCE: This study reports the design of nanocomposite hydrogels with two distinct co-assembling domains for structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy.
32084599	7	104	theme	drugs	2011:2015	arg1	release					1996:2002	differential release	1983:2002	differential release of two drugs (CDDP and IRN) in combination therapy	1983:2053	STATEMENT OF SIGNIFICANCE: This study reports the design of nanocomposite hydrogels with two distinct co-assembling domains for structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy.
32084599	1	105	theme	successful	158:167	arg1	therapy					181:187	a successful combination therapy	156:187	a successful combination therapy that utilizes cisplatin (CDDP) and irinotecan (IRN), with clinical evidence supporting a higher response rate when CDDP was administered prior to IRN	156:337	Temporal control of drug dosing is indispensable for a successful combination therapy that utilizes cisplatin (CDDP) and irinotecan (IRN), with clinical evidence supporting a higher response rate when CDDP was administered prior to IRN.
32084599	4	106	theme	drugs	988:992	arg1	release					972:978	controlled release	961:978	controlled release of dual drugs	961:992	Next, the nanocomposite hydrogel with excellent injectability served as a depot for controlled release of dual drugs, and guaranteed a fast release of CDDP prior to a tunable release of IRN that is dependent on fraction ratios of AlgNP in the composite materials.
32084599	6	107	theme	clinical	1771:1778	arg1	demands					1780:1786	clinical demands	1771:1786	clinical demands from combination therapy	1771:1811	We believe that the strategy of utilizing co-assembly of multiple pairs of entities (i.e. drug-gelator, nanoparticle-gelator) in composite materials provides a generalized method to design mechanically stable supramolecular hydrogels, and further promises an exact temporal control of drug dosing by packing individual drugs in co-assembled structures/domains to satisfy clinical demands from combination therapy.
32084599	10	108	theme	dosing	2610:2615	arg1	control					2594:2600	temporal control	2585:2600	temporal control of drug dosing	2585:2615	We believe that the strategy of packing individual drugs in distinct co-assembling structures promises a paradigm shift for regulating temporal control of drug dosing in combination therapy.
32084599	6	109	theme	exact	1659:1663	arg1	control					1674:1680	an exact temporal control	1656:1680	an exact temporal control of drug dosing	1656:1695	We believe that the strategy of utilizing co-assembly of multiple pairs of entities (i.e. drug-gelator, nanoparticle-gelator) in composite materials provides a generalized method to design mechanically stable supramolecular hydrogels, and further promises an exact temporal control of drug dosing by packing individual drugs in co-assembled structures/domains to satisfy clinical demands from combination therapy.
32084599	7	110	from	reinforcement	1953:1965	arg1	therapy					2047:2053	combination therapy	2035:2053	combination therapy	2035:2053	STATEMENT OF SIGNIFICANCE: This study reports the design of nanocomposite hydrogels with two distinct co-assembling domains for structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy.
32084599	10	111	from	drugs	2501:2505	arg1	structures					2533:2542	distinct co-assembling structures	2510:2542	distinct co-assembling structures	2510:2542	We believe that the strategy of packing individual drugs in distinct co-assembling structures promises a paradigm shift for regulating temporal control of drug dosing in combination therapy.
32084599	3	112	theme	storage	829:835	arg1	modulus					837:843	storage modulus	829:843	storage modulus comparing to peptide gel alone	829:874	First, a double-crosslinking strategy was exploited for structural reinforcement of hydrogel, with integration of coordination interactions between CDDP and hydrogelator (Pept) as well as electrostatic interactions between Pept and alginate nanoparticles (AlgNP/IRN), that afforded nanocomposite hydrogel with 42-fold increase in storage modulus comparing to peptide gel alone.
32084599	7	113	theme	differential	1983:1994	arg1	release					1996:2002	differential release	1983:2002	differential release of two drugs (CDDP and IRN) in combination therapy	1983:2053	STATEMENT OF SIGNIFICANCE: This study reports the design of nanocomposite hydrogels with two distinct co-assembling domains for structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy.
32084599	2	114	theme	drugs	492:496	arg1	synergism					475:483	synergism	475:483	synergism of two drugs	475:496	Herein, a peptide-based nanocomposite hydrogel (CDDP/Pept-AlgNP/IRN) was designed for differential release of CDDP and IRN to maximize synergism of two drugs.
32084599	6	115	theme	co-assembled	1728:1739	arg1	structures/domains					1741:1758	co-assembled structures/domains	1728:1758	co-assembled structures/domains	1728:1758	We believe that the strategy of utilizing co-assembly of multiple pairs of entities (i.e. drug-gelator, nanoparticle-gelator) in composite materials provides a generalized method to design mechanically stable supramolecular hydrogels, and further promises an exact temporal control of drug dosing by packing individual drugs in co-assembled structures/domains to satisfy clinical demands from combination therapy.
32084599	5	116	theme	antitumor	1330:1338	arg1	potency					1340:1346	efficacious antitumor potency further	1318:1354	efficacious antitumor potency further proved in tumor regression studies in vivo	1318:1397	Comparing to simple mixture of CDDP and IRN solution, CDDP/Pept-AlgNP/IRN hydrogel formulation demonstrated excelling synergism of CDDP and IRN in cell inhibition studies, with efficacious antitumor potency further proved in tumor regression studies in vivo.
32084599	7	117	theme	hydrogel	1970:1977	arg1	reinforcement					1953:1965	structural reinforcement	1942:1965	structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy	1942:2053	STATEMENT OF SIGNIFICANCE: This study reports the design of nanocomposite hydrogels with two distinct co-assembling domains for structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy.
32084599	5	118	theme	cell	1288:1291	arg1	studies					1304:1310	cell inhibition studies	1288:1310	cell inhibition studies	1288:1310	Comparing to simple mixture of CDDP and IRN solution, CDDP/Pept-AlgNP/IRN hydrogel formulation demonstrated excelling synergism of CDDP and IRN in cell inhibition studies, with efficacious antitumor potency further proved in tumor regression studies in vivo.
32084599	2	119	theme	peptide-based	350:362	arg1	hydrogel					378:385	a peptide-based nanocomposite hydrogel	348:385	a peptide-based nanocomposite hydrogel (CDDP/Pept-AlgNP/IRN)	348:407	Herein, a peptide-based nanocomposite hydrogel (CDDP/Pept-AlgNP/IRN) was designed for differential release of CDDP and IRN to maximize synergism of two drugs.
32084599	2	119	theme	peptide-based	350:362	arg1	CDDP/Pept-AlgNP/IRN					388:406	CDDP/Pept-AlgNP/IRN	388:406	CDDP/Pept-AlgNP/IRN	388:406	Herein, a peptide-based nanocomposite hydrogel (CDDP/Pept-AlgNP/IRN) was designed for differential release of CDDP and IRN to maximize synergism of two drugs.
32084599	5	120	theme	simple	1154:1159	arg1	mixture					1161:1167	simple mixture	1154:1167	simple mixture of CDDP and IRN solution	1154:1192	Comparing to simple mixture of CDDP and IRN solution, CDDP/Pept-AlgNP/IRN hydrogel formulation demonstrated excelling synergism of CDDP and IRN in cell inhibition studies, with efficacious antitumor potency further proved in tumor regression studies in vivo.
32084599	4	121	theme	CDDP	1028:1031	arg1	depot					951:955	a depot	949:955	a depot for controlled release of dual drugs	949:992	Next, the nanocomposite hydrogel with excellent injectability served as a depot for controlled release of dual drugs, and guaranteed a fast release of CDDP prior to a tunable release of IRN that is dependent on fraction ratios of AlgNP in the composite materials.
32084599	4	121	theme	CDDP	1028:1031	arg1	hydrogel					901:908	the nanocomposite hydrogel	883:908	the nanocomposite hydrogel with excellent injectability	883:937	Next, the nanocomposite hydrogel with excellent injectability served as a depot for controlled release of dual drugs, and guaranteed a fast release of CDDP prior to a tunable release of IRN that is dependent on fraction ratios of AlgNP in the composite materials.
32084599	4	121	theme	CDDP	1028:1031	arg1	release					1017:1023	a fast release	1010:1023	a fast release of CDDP	1010:1031	Next, the nanocomposite hydrogel with excellent injectability served as a depot for controlled release of dual drugs, and guaranteed a fast release of CDDP prior to a tunable release of IRN that is dependent on fraction ratios of AlgNP in the composite materials.
32084599	10	122	theme	drugs	2501:2505	arg1	strategy					2470:2477	the strategy	2466:2477	the strategy of packing individual drugs in distinct co-assembling structures	2466:2542	We believe that the strategy of packing individual drugs in distinct co-assembling structures promises a paradigm shift for regulating temporal control of drug dosing in combination therapy.
32084599	1	123	theme	response	285:292	arg1	rate					294:297	a higher response rate	276:297	a higher response rate when CDDP was administered prior to IRN	276:337	Temporal control of drug dosing is indispensable for a successful combination therapy that utilizes cisplatin (CDDP) and irinotecan (IRN), with clinical evidence supporting a higher response rate when CDDP was administered prior to IRN.
32084599	2	124	theme	IRN	459:461	arg1	release					439:445	differential release	426:445	differential release of CDDP and IRN	426:461	Herein, a peptide-based nanocomposite hydrogel (CDDP/Pept-AlgNP/IRN) was designed for differential release of CDDP and IRN to maximize synergism of two drugs.
32084599	5	125	theme	regression	1372:1381	arg1	studies					1383:1389	tumor regression studies	1366:1389	tumor regression studies	1366:1389	Comparing to simple mixture of CDDP and IRN solution, CDDP/Pept-AlgNP/IRN hydrogel formulation demonstrated excelling synergism of CDDP and IRN in cell inhibition studies, with efficacious antitumor potency further proved in tumor regression studies in vivo.
32084599	4	126	theme	controlled	961:970	arg1	release					972:978	controlled release	961:978	controlled release of dual drugs	961:992	Next, the nanocomposite hydrogel with excellent injectability served as a depot for controlled release of dual drugs, and guaranteed a fast release of CDDP prior to a tunable release of IRN that is dependent on fraction ratios of AlgNP in the composite materials.
32084599	0	127	theme	drug	67:70	arg1	dosing					72:77	drug dosing	67:77	drug dosing	67:77	Double-crosslinked nanocomposite hydrogels for temporal control of drug dosing in combination therapy.
32084599	7	128	theme	co-assembling	1916:1928	arg1	domains					1930:1936	two distinct co-assembling domains	1903:1936	two distinct co-assembling domains for structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy	1903:2053	STATEMENT OF SIGNIFICANCE: This study reports the design of nanocomposite hydrogels with two distinct co-assembling domains for structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy.
32084599	0	129	from	control	56:62	arg1	therapy					94:100	combination therapy	82:100	combination therapy	82:100	Double-crosslinked nanocomposite hydrogels for temporal control of drug dosing in combination therapy.
32084599	2	130	theme	CDDP	450:453	arg1	release					439:445	differential release	426:445	differential release of CDDP and IRN	426:461	Herein, a peptide-based nanocomposite hydrogel (CDDP/Pept-AlgNP/IRN) was designed for differential release of CDDP and IRN to maximize synergism of two drugs.
32084599	9	131	dep	cell	2306:2309	arg1	A549					2311:2314	A549	2311:2314	A549	2311:2314	Then we utilized the hydrogel for differential release of CDDP and IRN to achieve better synergistic efficacy of drugs in inhibiting the growth of cancer cell A549 and better anticancer efficacies than single drug formulations or solution mixtures of dual drugs in an A549-xenografted mouse model.
32084599	7	132	theme	nanocomposite	1874:1886	arg1	hydrogels					1888:1896	nanocomposite hydrogels	1874:1896	nanocomposite hydrogels	1874:1896	STATEMENT OF SIGNIFICANCE: This study reports the design of nanocomposite hydrogels with two distinct co-assembling domains for structural reinforcement of hydrogel and differential release of two drugs (CDDP and IRN) in combination therapy.
32084599	3	133	from	increase	817:824	arg1	modulus					837:843	storage modulus	829:843	storage modulus comparing to peptide gel alone	829:874	First, a double-crosslinking strategy was exploited for structural reinforcement of hydrogel, with integration of coordination interactions between CDDP and hydrogelator (Pept) as well as electrostatic interactions between Pept and alginate nanoparticles (AlgNP/IRN), that afforded nanocomposite hydrogel with 42-fold increase in storage modulus comparing to peptide gel alone.
32084599	5	134	theme	further	1348:1354	arg1	potency					1340:1346	efficacious antitumor potency further	1318:1354	efficacious antitumor potency further proved in tumor regression studies in vivo	1318:1397	Comparing to simple mixture of CDDP and IRN solution, CDDP/Pept-AlgNP/IRN hydrogel formulation demonstrated excelling synergism of CDDP and IRN in cell inhibition studies, with efficacious antitumor potency further proved in tumor regression studies in vivo.
32084599	6	135	theme	entities	1475:1482	arg1	pairs					1466:1470	multiple pairs	1457:1470	multiple pairs of entities (i.e. drug-gelator, nanoparticle-gelator)	1457:1524	We believe that the strategy of utilizing co-assembly of multiple pairs of entities (i.e. drug-gelator, nanoparticle-gelator) in composite materials provides a generalized method to design mechanically stable supramolecular hydrogels, and further promises an exact temporal control of drug dosing by packing individual drugs in co-assembled structures/domains to satisfy clinical demands from combination therapy.
32084599	5	136	theme	CDDP/Pept-AlgNP/IRN	1195:1213	arg1	formulation					1224:1234	CDDP/Pept-AlgNP/IRN hydrogel formulation	1195:1234	CDDP/Pept-AlgNP/IRN hydrogel formulation	1195:1234	Comparing to simple mixture of CDDP and IRN solution, CDDP/Pept-AlgNP/IRN hydrogel formulation demonstrated excelling synergism of CDDP and IRN in cell inhibition studies, with efficacious antitumor potency further proved in tumor regression studies in vivo.
32084599	4	137	from	materials	1130:1138	arg1	ratios					1097:1102	fraction ratios	1088:1102	fraction ratios of AlgNP in the composite materials	1088:1138	Next, the nanocomposite hydrogel with excellent injectability served as a depot for controlled release of dual drugs, and guaranteed a fast release of CDDP prior to a tunable release of IRN that is dependent on fraction ratios of AlgNP in the composite materials.
32084599	3	138	theme	electrostatic	687:699	arg1	interactions					701:712	electrostatic interactions	687:712	coordination interactions between CDDP and hydrogelator (Pept) as well as electrostatic interactions between Pept and alginate nanoparticles (AlgNP/IRN)	613:764	First, a double-crosslinking strategy was exploited for structural reinforcement of hydrogel, with integration of coordination interactions between CDDP and hydrogelator (Pept) as well as electrostatic interactions between Pept and alginate nanoparticles (AlgNP/IRN), that afforded nanocomposite hydrogel with 42-fold increase in storage modulus comparing to peptide gel alone.
32084599	9	139	theme	dual	2403:2406	arg1	drugs					2408:2412	dual drugs	2403:2412	dual drugs	2403:2412	Then we utilized the hydrogel for differential release of CDDP and IRN to achieve better synergistic efficacy of drugs in inhibiting the growth of cancer cell A549 and better anticancer efficacies than single drug formulations or solution mixtures of dual drugs in an A549-xenografted mouse model.
32084599	6	140	theme	pairs	1466:1470	arg1	co-assembly					1442:1452	co-assembly	1442:1452	co-assembly of multiple pairs of entities (i.e. drug-gelator, nanoparticle-gelator) in composite materials	1442:1547	We believe that the strategy of utilizing co-assembly of multiple pairs of entities (i.e. drug-gelator, nanoparticle-gelator) in composite materials provides a generalized method to design mechanically stable supramolecular hydrogels, and further promises an exact temporal control of drug dosing by packing individual drugs in co-assembled structures/domains to satisfy clinical demands from combination therapy.
32084599	9	141	theme	synergistic	2241:2251	arg1	efficacy					2253:2260	better synergistic efficacy	2234:2260	better synergistic efficacy of drugs	2234:2269	Then we utilized the hydrogel for differential release of CDDP and IRN to achieve better synergistic efficacy of drugs in inhibiting the growth of cancer cell A549 and better anticancer efficacies than single drug formulations or solution mixtures of dual drugs in an A549-xenografted mouse model.
33157135	0	0	theme	fresh	92:96	arg1	apples					102:107	fresh cut apples	92:107	fresh cut apples	92:107	Optimization, characterization and evaluation of papaya polysaccharide-corn starch film for fresh cut apples.
33157135	2	1	theme	extraction	323:332	arg1	duration					334:341	extraction duration	323:341	extraction duration	323:341	A Box-Behnken experimental design was used to determine the effect of ethanol concentration, extraction duration, and material concentration during PPs extraction.
33157135	1	2	theme	casting	211:217	arg1	technique					219:227	the solution casting technique	198:227	the solution casting technique	198:227	In this study, we prepared corn starch (CS) and papaya polysaccharide (PPs) films using the solution casting technique.
33157135	6	3	theme	SEM	896:898	arg1	images					900:905	SEM images	896:905	SEM images	896:905	SEM images revealed that PPs and CS are highly compatible; moreover, FTIR spectroscopy showed that intermolecular hydrogen bonding existed between CS and PPs, forming a compact film structure.
33157135	4	4	theme	antibacterial	675:687	arg1	performance					689:699	general antibacterial performance	667:699	general antibacterial performance	667:699	The results show that PPs-CS composite films have good antioxidant and moisturizing properties and general antibacterial performance.
33157135	6	5	theme	FTIR	965:968	arg1	spectroscopy					970:981	FTIR spectroscopy	965:981	FTIR spectroscopy	965:981	SEM images revealed that PPs and CS are highly compatible; moreover, FTIR spectroscopy showed that intermolecular hydrogen bonding existed between CS and PPs, forming a compact film structure.
33157135	7	6	theme	cut	1165:1167	arg1	apples					1169:1174	fresh cut apples	1159:1174	fresh cut apples	1159:1174	Finally, the incorporation of PPs and CS influenced the shelf-life of fresh cut apples, with the edible film incorporated with PPs positively improving sensory acceptance of combined materials.
33157135	7	7	theme	sensory	1241:1247	arg1	acceptance					1249:1258	sensory acceptance	1241:1258	sensory acceptance of combined materials	1241:1280	Finally, the incorporation of PPs and CS influenced the shelf-life of fresh cut apples, with the edible film incorporated with PPs positively improving sensory acceptance of combined materials.
33157135	0	8	theme	cut	98:100	arg1	apples					102:107	fresh cut apples	92:107	fresh cut apples	92:107	Optimization, characterization and evaluation of papaya polysaccharide-corn starch film for fresh cut apples.
33157135	6	9	theme	compact	1065:1071	arg1	structure					1078:1086	a compact film structure	1063:1086	a compact film structure	1063:1086	SEM images revealed that PPs and CS are highly compatible; moreover, FTIR spectroscopy showed that intermolecular hydrogen bonding existed between CS and PPs, forming a compact film structure.
33157135	3	10	theme	physical	465:472	arg1	spectroscopy					530:541	physical, optical, mechanical, Fourier-transform infrared (FTIR) spectroscopy	465:541	physical, optical, mechanical, Fourier-transform infrared (FTIR) spectroscopy	465:541	The resulting films were characterized in terms of structural changes, physical, optical, mechanical, Fourier-transform infrared (FTIR) spectroscopy, and thermal properties.
33157135	4	11	theme	composite	597:605	arg1	films					607:611	PPs-CS composite films	590:611	PPs-CS composite films	590:611	The results show that PPs-CS composite films have good antioxidant and moisturizing properties and general antibacterial performance.
33157135	2	12	theme	experimental	244:255	arg1	design					257:262	A Box-Behnken experimental design	230:262	A Box-Behnken experimental design	230:262	A Box-Behnken experimental design was used to determine the effect of ethanol concentration, extraction duration, and material concentration during PPs extraction.
33157135	3	13	theme	resulting	398:406	arg1	films					408:412	The resulting films	394:412	The resulting films	394:412	The resulting films were characterized in terms of structural changes, physical, optical, mechanical, Fourier-transform infrared (FTIR) spectroscopy, and thermal properties.
33157135	7	14	theme	fresh	1159:1163	arg1	apples					1169:1174	fresh cut apples	1159:1174	fresh cut apples	1159:1174	Finally, the incorporation of PPs and CS influenced the shelf-life of fresh cut apples, with the edible film incorporated with PPs positively improving sensory acceptance of combined materials.
33157135	2	15	theme	Box-Behnken	232:242	arg1	design					257:262	A Box-Behnken experimental design	230:262	A Box-Behnken experimental design	230:262	A Box-Behnken experimental design was used to determine the effect of ethanol concentration, extraction duration, and material concentration during PPs extraction.
33157135	2	16	theme	concentration	357:369	arg1	effect					290:295	the effect	286:295	the effect of ethanol concentration, extraction duration, and material concentration during PPs extraction	286:391	A Box-Behnken experimental design was used to determine the effect of ethanol concentration, extraction duration, and material concentration during PPs extraction.
33157135	2	17	theme	material	348:355	arg1	concentration					357:369	material concentration	348:369	material concentration	348:369	A Box-Behnken experimental design was used to determine the effect of ethanol concentration, extraction duration, and material concentration during PPs extraction.
33157135	1	18	theme	papaya	158:163	arg1	PPs					181:183	PPs	181:183	PPs	181:183	In this study, we prepared corn starch (CS) and papaya polysaccharide (PPs) films using the solution casting technique.
33157135	1	18	theme	papaya	158:163	arg1	polysaccharide					165:178	papaya polysaccharide	158:178	papaya polysaccharide (PPs)	158:184	In this study, we prepared corn starch (CS) and papaya polysaccharide (PPs) films using the solution casting technique.
33157135	5	19	theme	tensile	807:813	arg1	strength					815:822	tensile strength	807:822	tensile strength	807:822	These results revealed that after adding PPs, the films exhibited a significant increase in swelling and tensile strength, while depicted a reduction in thickness, transparency, and solubility.
33157135	1	20	dep	prepared	128:135	arg1	using					192:196	using	192:196	using the solution casting technique	192:227	In this study, we prepared corn starch (CS) and papaya polysaccharide (PPs) films using the solution casting technique.
33157135	7	21	theme	materials	1272:1280	arg1	acceptance					1249:1258	sensory acceptance	1241:1258	sensory acceptance of combined materials	1241:1280	Finally, the incorporation of PPs and CS influenced the shelf-life of fresh cut apples, with the edible film incorporated with PPs positively improving sensory acceptance of combined materials.
33157135	7	22	theme	combined	1263:1270	arg1	materials					1272:1280	combined materials	1263:1280	combined materials	1263:1280	Finally, the incorporation of PPs and CS influenced the shelf-life of fresh cut apples, with the edible film incorporated with PPs positively improving sensory acceptance of combined materials.
33157135	3	23	dep	physical	465:472	arg1	Fourier-transform					496:512	Fourier-transform	496:512	Fourier-transform	496:512	The resulting films were characterized in terms of structural changes, physical, optical, mechanical, Fourier-transform infrared (FTIR) spectroscopy, and thermal properties.
33157135	3	23	dep	physical	465:472	arg1	mechanical					484:493	mechanical	484:493	mechanical	484:493	The resulting films were characterized in terms of structural changes, physical, optical, mechanical, Fourier-transform infrared (FTIR) spectroscopy, and thermal properties.
33157135	3	23	dep	physical	465:472	arg1	optical					475:481	optical	475:481	optical	475:481	The resulting films were characterized in terms of structural changes, physical, optical, mechanical, Fourier-transform infrared (FTIR) spectroscopy, and thermal properties.
33157135	4	24	contain	have	613:616	arg2	properties					652:661	good antioxidant and moisturizing properties	618:661	good antioxidant and moisturizing properties	618:661	The results show that PPs-CS composite films have good antioxidant and moisturizing properties and general antibacterial performance.
33157135	4	24	contain	have	613:616	arg2	performance					689:699	general antibacterial performance	667:699	general antibacterial performance	667:699	The results show that PPs-CS composite films have good antioxidant and moisturizing properties and general antibacterial performance.
33157135	4	24	contain	have	613:616	arg1	films					607:611	PPs-CS composite films	590:611	PPs-CS composite films	590:611	The results show that PPs-CS composite films have good antioxidant and moisturizing properties and general antibacterial performance.
33157135	3	25	theme	spectroscopy	530:541	arg1	terms					436:440	terms	436:440	terms of structural changes, physical, optical, mechanical, Fourier-transform infrared (FTIR) spectroscopy, and thermal properties	436:565	The resulting films were characterized in terms of structural changes, physical, optical, mechanical, Fourier-transform infrared (FTIR) spectroscopy, and thermal properties.
33157135	3	26	theme	structural	445:454	arg1	changes					456:462	structural changes	445:462	structural changes	445:462	The resulting films were characterized in terms of structural changes, physical, optical, mechanical, Fourier-transform infrared (FTIR) spectroscopy, and thermal properties.
33157135	7	27	theme	PPs	1119:1121	arg1	incorporation					1102:1114	the incorporation	1098:1114	the incorporation of PPs	1098:1121	Finally, the incorporation of PPs and CS influenced the shelf-life of fresh cut apples, with the edible film incorporated with PPs positively improving sensory acceptance of combined materials.
33157135	7	27	theme	PPs	1119:1121	arg1	CS					1127:1128	CS	1127:1128	CS	1127:1128	Finally, the incorporation of PPs and CS influenced the shelf-life of fresh cut apples, with the edible film incorporated with PPs positively improving sensory acceptance of combined materials.
33157135	3	28	dep	infrared	514:521	arg1	FTIR					524:527	FTIR	524:527	FTIR	524:527	The resulting films were characterized in terms of structural changes, physical, optical, mechanical, Fourier-transform infrared (FTIR) spectroscopy, and thermal properties.
33157135	4	29	theme	moisturizing	639:650	arg1	properties					652:661	good antioxidant and moisturizing properties	618:661	good antioxidant and moisturizing properties	618:661	The results show that PPs-CS composite films have good antioxidant and moisturizing properties and general antibacterial performance.
33157135	4	30	theme	good	618:621	arg1	properties					652:661	good antioxidant and moisturizing properties	618:661	good antioxidant and moisturizing properties	618:661	The results show that PPs-CS composite films have good antioxidant and moisturizing properties and general antibacterial performance.
33157135	5	31	theme	significant	770:780	arg1	increase					782:789	a significant increase	768:789	a significant increase in swelling and tensile strength	768:822	These results revealed that after adding PPs, the films exhibited a significant increase in swelling and tensile strength, while depicted a reduction in thickness, transparency, and solubility.
33157135	2	32	theme	PPs	378:380	arg1	extraction					382:391	PPs extraction	378:391	PPs extraction	378:391	A Box-Behnken experimental design was used to determine the effect of ethanol concentration, extraction duration, and material concentration during PPs extraction.
33157135	0	33	theme	polysaccharide-corn	56:74	arg1	film					83:86	papaya polysaccharide-corn starch film	49:86	papaya polysaccharide-corn starch film	49:86	Optimization, characterization and evaluation of papaya polysaccharide-corn starch film for fresh cut apples.
33157135	7	34	theme	edible	1186:1191	arg1	film					1193:1196	the edible film	1182:1196	the edible film incorporated with PPs positively improving sensory acceptance of combined materials	1182:1280	Finally, the incorporation of PPs and CS influenced the shelf-life of fresh cut apples, with the edible film incorporated with PPs positively improving sensory acceptance of combined materials.
33157135	3	35	theme	thermal	548:554	arg1	properties					556:565	thermal properties	548:565	thermal properties	548:565	The resulting films were characterized in terms of structural changes, physical, optical, mechanical, Fourier-transform infrared (FTIR) spectroscopy, and thermal properties.
33157135	2	36	theme	concentration	308:320	arg1	effect					290:295	the effect	286:295	the effect of ethanol concentration, extraction duration, and material concentration during PPs extraction	286:391	A Box-Behnken experimental design was used to determine the effect of ethanol concentration, extraction duration, and material concentration during PPs extraction.
33157135	0	37	theme	papaya	49:54	arg1	film					83:86	papaya polysaccharide-corn starch film	49:86	papaya polysaccharide-corn starch film	49:86	Optimization, characterization and evaluation of papaya polysaccharide-corn starch film for fresh cut apples.
33157135	3	38	theme	properties	556:565	arg1	terms					436:440	terms	436:440	terms of structural changes, physical, optical, mechanical, Fourier-transform infrared (FTIR) spectroscopy, and thermal properties	436:565	The resulting films were characterized in terms of structural changes, physical, optical, mechanical, Fourier-transform infrared (FTIR) spectroscopy, and thermal properties.
33157135	7	39	theme	apples	1169:1174	arg1	shelf-life					1145:1154	the shelf-life	1141:1154	the shelf-life of fresh cut apples	1141:1174	Finally, the incorporation of PPs and CS influenced the shelf-life of fresh cut apples, with the edible film incorporated with PPs positively improving sensory acceptance of combined materials.
33157135	2	40	theme	ethanol	300:306	arg1	concentration					308:320	ethanol concentration	300:320	ethanol concentration	300:320	A Box-Behnken experimental design was used to determine the effect of ethanol concentration, extraction duration, and material concentration during PPs extraction.
33157135	2	41	used	used	268:271	arg2	design					257:262	A Box-Behnken experimental design	230:262	A Box-Behnken experimental design	230:262	A Box-Behnken experimental design was used to determine the effect of ethanol concentration, extraction duration, and material concentration during PPs extraction.
33157135	3	42	theme	changes	456:462	arg1	terms					436:440	terms	436:440	terms of structural changes, physical, optical, mechanical, Fourier-transform infrared (FTIR) spectroscopy, and thermal properties	436:565	The resulting films were characterized in terms of structural changes, physical, optical, mechanical, Fourier-transform infrared (FTIR) spectroscopy, and thermal properties.
33157135	6	43	theme	hydrogen	1010:1017	arg1	bonding					1019:1025	intermolecular hydrogen bonding	995:1025	intermolecular hydrogen bonding	995:1025	SEM images revealed that PPs and CS are highly compatible; moreover, FTIR spectroscopy showed that intermolecular hydrogen bonding existed between CS and PPs, forming a compact film structure.
33157135	3	44	theme	infrared	514:521	arg1	spectroscopy					530:541	physical, optical, mechanical, Fourier-transform infrared (FTIR) spectroscopy	465:541	physical, optical, mechanical, Fourier-transform infrared (FTIR) spectroscopy	465:541	The resulting films were characterized in terms of structural changes, physical, optical, mechanical, Fourier-transform infrared (FTIR) spectroscopy, and thermal properties.
33157135	1	45	theme	corn	137:140	arg1	CS					150:151	CS	150:151	CS	150:151	In this study, we prepared corn starch (CS) and papaya polysaccharide (PPs) films using the solution casting technique.
33157135	1	45	theme	corn	137:140	arg1	starch					142:147	corn starch	137:147	corn starch (CS)	137:152	In this study, we prepared corn starch (CS) and papaya polysaccharide (PPs) films using the solution casting technique.
33157135	6	46	theme	intermolecular	995:1008	arg1	bonding					1019:1025	intermolecular hydrogen bonding	995:1025	intermolecular hydrogen bonding	995:1025	SEM images revealed that PPs and CS are highly compatible; moreover, FTIR spectroscopy showed that intermolecular hydrogen bonding existed between CS and PPs, forming a compact film structure.
33157135	0	47	theme	film	83:86	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Optimization, characterization and evaluation of papaya polysaccharide-corn starch film for fresh cut apples.
33157135	0	47	theme	film	83:86	arg1	Optimization					0:11	Optimization	0:11	Optimization	0:11	Optimization, characterization and evaluation of papaya polysaccharide-corn starch film for fresh cut apples.
33157135	0	47	theme	film	83:86	arg1	evaluation					35:44	evaluation	35:44	evaluation	35:44	Optimization, characterization and evaluation of papaya polysaccharide-corn starch film for fresh cut apples.
33157135	6	48	theme	film	1073:1076	arg1	structure					1078:1086	a compact film structure	1063:1086	a compact film structure	1063:1086	SEM images revealed that PPs and CS are highly compatible; moreover, FTIR spectroscopy showed that intermolecular hydrogen bonding existed between CS and PPs, forming a compact film structure.
33157135	4	49	theme	general	667:673	arg1	performance					689:699	general antibacterial performance	667:699	general antibacterial performance	667:699	The results show that PPs-CS composite films have good antioxidant and moisturizing properties and general antibacterial performance.
33157135	4	50	theme	antioxidant	623:633	arg1	properties					652:661	good antioxidant and moisturizing properties	618:661	good antioxidant and moisturizing properties	618:661	The results show that PPs-CS composite films have good antioxidant and moisturizing properties and general antibacterial performance.
33157135	4	51	theme	PPs-CS	590:595	arg1	films					607:611	PPs-CS composite films	590:611	PPs-CS composite films	590:611	The results show that PPs-CS composite films have good antioxidant and moisturizing properties and general antibacterial performance.
33157135	0	52	theme	starch	76:81	arg1	film					83:86	papaya polysaccharide-corn starch film	49:86	papaya polysaccharide-corn starch film	49:86	Optimization, characterization and evaluation of papaya polysaccharide-corn starch film for fresh cut apples.
33157135	5	53	from	increase	782:789	arg1	swelling					794:801	swelling	794:801	swelling	794:801	These results revealed that after adding PPs, the films exhibited a significant increase in swelling and tensile strength, while depicted a reduction in thickness, transparency, and solubility.
33157135	5	53	from	increase	782:789	arg1	strength					815:822	tensile strength	807:822	tensile strength	807:822	These results revealed that after adding PPs, the films exhibited a significant increase in swelling and tensile strength, while depicted a reduction in thickness, transparency, and solubility.
33157135	5	54	from	reduction	842:850	arg1	transparency					866:877	transparency	866:877	transparency	866:877	These results revealed that after adding PPs, the films exhibited a significant increase in swelling and tensile strength, while depicted a reduction in thickness, transparency, and solubility.
33157135	5	54	from	reduction	842:850	arg1	solubility					884:893	solubility	884:893	solubility	884:893	These results revealed that after adding PPs, the films exhibited a significant increase in swelling and tensile strength, while depicted a reduction in thickness, transparency, and solubility.
33157135	5	54	from	reduction	842:850	arg1	thickness					855:863	thickness	855:863	thickness	855:863	These results revealed that after adding PPs, the films exhibited a significant increase in swelling and tensile strength, while depicted a reduction in thickness, transparency, and solubility.
33157135	2	55	theme	duration	334:341	arg1	effect					290:295	the effect	286:295	the effect of ethanol concentration, extraction duration, and material concentration during PPs extraction	286:391	A Box-Behnken experimental design was used to determine the effect of ethanol concentration, extraction duration, and material concentration during PPs extraction.
33157135	1	56	theme	solution	202:209	arg1	technique					219:227	the solution casting technique	198:227	the solution casting technique	198:227	In this study, we prepared corn starch (CS) and papaya polysaccharide (PPs) films using the solution casting technique.
34087297	2	0	theme	PEGylated	476:484	arg1	CNCs					486:489	PEGylated CNCs	476:489	PEGylated CNCs	476:489	DOX could be loaded on PEGylated CNCs with high capacity and released from drug complexes (P-CNCs-D) with pH dependent behavior.
34087297	1	1	theme	polyethylene	356:367	arg1	CHO-PEG					377:383	CHO-PEG	377:383	CHO-PEG	377:383	Herein, a novel strategy for surface functionalization and drug loading of cellulose nanocrystals (CNCs) through formation of hydrazone bonds between functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX) was reported for the first time.
34087297	1	1	theme	polyethylene	356:367	arg1	glycol					369:374	polyethylene glycol	356:374	polyethylene glycol (CHO-PEG)	356:384	Herein, a novel strategy for surface functionalization and drug loading of cellulose nanocrystals (CNCs) through formation of hydrazone bonds between functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX) was reported for the first time.
34087297	4	2	from	simultaneous	896:907	arg1	route					969:973	a one-pot route	959:973	a one-pot route	959:973	As compared with other method, the method developed in this work is rather simple and effective and can be achieved for simultaneous for surface functionalization and drug loading in a one-pot route.
34087297	5	3	theme	composites	1052:1061	arg1	fabrication					1013:1023	fabrication	1013:1023	fabrication of various multifunctional composites based on other carbohydrate polymers or materials	1013:1111	This work will open a new avenue for fabrication of various multifunctional composites based on other carbohydrate polymers or materials and to explore their applications in biomedical fields.
34087297	0	4	theme	cellulose	91:99	arg1	system					148:153	cellulose nanocrystals-based pH responsive drug delivery system	91:153	cellulose nanocrystals-based pH responsive drug delivery system	91:153	Simultaneous surface functionalization and drug loading: A novel method for fabrication of cellulose nanocrystals-based pH responsive drug delivery system.
34087297	5	5	from	applications	1134:1145	arg1	fields					1161:1166	biomedical fields	1150:1166	biomedical fields	1150:1166	This work will open a new avenue for fabrication of various multifunctional composites based on other carbohydrate polymers or materials and to explore their applications in biomedical fields.
34087297	1	6	theme	first	441:445	arg1	time					447:450	the first time	437:450	the first time	437:450	Herein, a novel strategy for surface functionalization and drug loading of cellulose nanocrystals (CNCs) through formation of hydrazone bonds between functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX) was reported for the first time.
34087297	5	7	theme	other	1072:1076	arg1	polymers					1091:1098	other carbohydrate polymers	1072:1098	other carbohydrate polymers	1072:1098	This work will open a new avenue for fabrication of various multifunctional composites based on other carbohydrate polymers or materials and to explore their applications in biomedical fields.
34087297	5	8	theme	carbohydrate	1078:1089	arg1	polymers					1091:1098	other carbohydrate polymers	1072:1098	other carbohydrate polymers	1072:1098	This work will open a new avenue for fabrication of various multifunctional composites based on other carbohydrate polymers or materials and to explore their applications in biomedical fields.
34087297	2	9	theme	dependent	562:570	arg1	behavior					572:579	pH dependent behavior	559:579	pH dependent behavior	559:579	DOX could be loaded on PEGylated CNCs with high capacity and released from drug complexes (P-CNCs-D) with pH dependent behavior.
34087297	0	10	theme	pH	120:121	arg1	system					148:153	cellulose nanocrystals-based pH responsive drug delivery system	91:153	cellulose nanocrystals-based pH responsive drug delivery system	91:153	Simultaneous surface functionalization and drug loading: A novel method for fabrication of cellulose nanocrystals-based pH responsive drug delivery system.
34087297	1	11	theme	novel	166:170	arg1	strategy					172:179	a novel strategy	164:179	a novel strategy for surface functionalization and drug loading of cellulose nanocrystals (CNCs) through formation of hydrazone bonds between functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX)	164:418	Herein, a novel strategy for surface functionalization and drug loading of cellulose nanocrystals (CNCs) through formation of hydrazone bonds between functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX) was reported for the first time.
34087297	0	12	theme	nanocrystals-based	101:118	arg1	system					148:153	cellulose nanocrystals-based pH responsive drug delivery system	91:153	cellulose nanocrystals-based pH responsive drug delivery system	91:153	Simultaneous surface functionalization and drug loading: A novel method for fabrication of cellulose nanocrystals-based pH responsive drug delivery system.
34087297	4	13	from	route	969:973	arg1	simultaneous					896:907	simultaneous	896:907	simultaneous	896:907	As compared with other method, the method developed in this work is rather simple and effective and can be achieved for simultaneous for surface functionalization and drug loading in a one-pot route.
34087297	0	14	theme	surface	13:19	arg1	functionalization					21:37	Simultaneous surface functionalization	0:37	Simultaneous surface functionalization	0:37	Simultaneous surface functionalization and drug loading: A novel method for fabrication of cellulose nanocrystals-based pH responsive drug delivery system.
34087297	1	15	theme	/anticancer	385:395	arg1	DOX					415:417	DOX	415:417	DOX	415:417	Herein, a novel strategy for surface functionalization and drug loading of cellulose nanocrystals (CNCs) through formation of hydrazone bonds between functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX) was reported for the first time.
34087297	1	15	theme	/anticancer	385:395	arg1	doxorubicin					402:412	functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin	306:412	functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX)	306:418	Herein, a novel strategy for surface functionalization and drug loading of cellulose nanocrystals (CNCs) through formation of hydrazone bonds between functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX) was reported for the first time.
34087297	4	16	theme	drug	943:946	arg1	loading					948:954	drug loading	943:954	drug loading	943:954	As compared with other method, the method developed in this work is rather simple and effective and can be achieved for simultaneous for surface functionalization and drug loading in a one-pot route.
34087297	0	17	theme	Simultaneous	0:11	arg1	functionalization					21:37	Simultaneous surface functionalization	0:37	Simultaneous surface functionalization	0:37	Simultaneous surface functionalization and drug loading: A novel method for fabrication of cellulose nanocrystals-based pH responsive drug delivery system.
34087297	3	18	theme	desirable	746:754	arg1	effects					767:773	desirable anticancer effects	746:773	desirable anticancer effects	746:773	The biological evaluation results demonstrated that drug carriers (CNCs-EBO-NH) showed negative cytotoxicity while DOX could be transported into cells and exhibits desirable anticancer effects.
34087297	1	19	theme	surface	185:191	arg1	functionalization					193:209	surface functionalization	185:209	surface functionalization	185:209	Herein, a novel strategy for surface functionalization and drug loading of cellulose nanocrystals (CNCs) through formation of hydrazone bonds between functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX) was reported for the first time.
34087297	2	20	theme	drug	528:531	arg1	complexes					533:541	drug complexes	528:541	drug complexes (P-CNCs-D)	528:552	DOX could be loaded on PEGylated CNCs with high capacity and released from drug complexes (P-CNCs-D) with pH dependent behavior.
34087297	2	20	theme	drug	528:531	arg1	P-CNCs-D					544:551	P-CNCs-D	544:551	P-CNCs-D	544:551	DOX could be loaded on PEGylated CNCs with high capacity and released from drug complexes (P-CNCs-D) with pH dependent behavior.
34087297	1	21	theme	hydrazone	282:290	arg1	bonds					292:296	hydrazone bonds	282:296	hydrazone bonds between functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX)	282:418	Herein, a novel strategy for surface functionalization and drug loading of cellulose nanocrystals (CNCs) through formation of hydrazone bonds between functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX) was reported for the first time.
34087297	0	22	theme	drug	134:137	arg1	system					148:153	cellulose nanocrystals-based pH responsive drug delivery system	91:153	cellulose nanocrystals-based pH responsive drug delivery system	91:153	Simultaneous surface functionalization and drug loading: A novel method for fabrication of cellulose nanocrystals-based pH responsive drug delivery system.
34087297	1	23	theme	bonds	292:296	arg1	formation					269:277	formation	269:277	formation of hydrazone bonds between functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX)	269:418	Herein, a novel strategy for surface functionalization and drug loading of cellulose nanocrystals (CNCs) through formation of hydrazone bonds between functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX) was reported for the first time.
34087297	0	24	theme	responsive	123:132	arg1	system					148:153	cellulose nanocrystals-based pH responsive drug delivery system	91:153	cellulose nanocrystals-based pH responsive drug delivery system	91:153	Simultaneous surface functionalization and drug loading: A novel method for fabrication of cellulose nanocrystals-based pH responsive drug delivery system.
34087297	1	25	theme	drug	397:400	arg1	DOX					415:417	DOX	415:417	DOX	415:417	Herein, a novel strategy for surface functionalization and drug loading of cellulose nanocrystals (CNCs) through formation of hydrazone bonds between functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX) was reported for the first time.
34087297	1	25	theme	drug	397:400	arg1	doxorubicin					402:412	functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin	306:412	functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX)	306:418	Herein, a novel strategy for surface functionalization and drug loading of cellulose nanocrystals (CNCs) through formation of hydrazone bonds between functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX) was reported for the first time.
34087297	0	26	theme	system	148:153	arg1	fabrication					76:86	fabrication	76:86	fabrication of cellulose nanocrystals-based pH responsive drug delivery system	76:153	Simultaneous surface functionalization and drug loading: A novel method for fabrication of cellulose nanocrystals-based pH responsive drug delivery system.
34087297	3	27	dep	results	608:614	arg1	demonstrated					616:627	demonstrated	616:627	results demonstrated that drug carriers (CNCs-EBO-NH) showed negative cytotoxicity while DOX could be transported into cells and exhibits desirable anticancer effects	608:773	The biological evaluation results demonstrated that drug carriers (CNCs-EBO-NH) showed negative cytotoxicity while DOX could be transported into cells and exhibits desirable anticancer effects.
34087297	1	28	theme	drug	215:218	arg1	loading					220:226	drug loading	215:226	drug loading	215:226	Herein, a novel strategy for surface functionalization and drug loading of cellulose nanocrystals (CNCs) through formation of hydrazone bonds between functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX) was reported for the first time.
34087297	0	29	theme	drug	43:46	arg1	loading					48:54	drug loading	43:54	drug loading	43:54	Simultaneous surface functionalization and drug loading: A novel method for fabrication of cellulose nanocrystals-based pH responsive drug delivery system.
34087297	1	30	theme	functionalized	306:319	arg1	CNCs					321:324	functionalized CNCs	306:324	functionalized CNCs	306:324	Herein, a novel strategy for surface functionalization and drug loading of cellulose nanocrystals (CNCs) through formation of hydrazone bonds between functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX) was reported for the first time.
34087297	0	31	theme	delivery	139:146	arg1	system					148:153	cellulose nanocrystals-based pH responsive drug delivery system	91:153	cellulose nanocrystals-based pH responsive drug delivery system	91:153	Simultaneous surface functionalization and drug loading: A novel method for fabrication of cellulose nanocrystals-based pH responsive drug delivery system.
34087297	5	32	theme	new	998:1000	arg1	avenue					1002:1007	a new avenue	996:1007	a new avenue for fabrication of various multifunctional composites based on other carbohydrate polymers or materials	996:1111	This work will open a new avenue for fabrication of various multifunctional composites based on other carbohydrate polymers or materials and to explore their applications in biomedical fields.
34087297	2	33	theme	high	496:499	arg1	capacity					501:508	high capacity	496:508	high capacity	496:508	DOX could be loaded on PEGylated CNCs with high capacity and released from drug complexes (P-CNCs-D) with pH dependent behavior.
34087297	1	34	theme	CNCs	321:324	arg1	DOX					415:417	DOX	415:417	DOX	415:417	Herein, a novel strategy for surface functionalization and drug loading of cellulose nanocrystals (CNCs) through formation of hydrazone bonds between functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX) was reported for the first time.
34087297	1	34	theme	CNCs	321:324	arg1	doxorubicin					402:412	functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin	306:412	functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX)	306:418	Herein, a novel strategy for surface functionalization and drug loading of cellulose nanocrystals (CNCs) through formation of hydrazone bonds between functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX) was reported for the first time.
34087297	3	35	theme	drug	634:637	arg1	carriers					639:646	drug carriers	634:646	drug carriers (CNCs-EBO-NH)	634:660	The biological evaluation results demonstrated that drug carriers (CNCs-EBO-NH) showed negative cytotoxicity while DOX could be transported into cells and exhibits desirable anticancer effects.
34087297	3	35	theme	drug	634:637	arg1	CNCs-EBO-NH					649:659	CNCs-EBO-NH	649:659	CNCs-EBO-NH	649:659	The biological evaluation results demonstrated that drug carriers (CNCs-EBO-NH) showed negative cytotoxicity while DOX could be transported into cells and exhibits desirable anticancer effects.
34087297	2	36	attach	released	514:521	arg1	complexes					533:541	drug complexes	528:541	drug complexes (P-CNCs-D)	528:552	DOX could be loaded on PEGylated CNCs with high capacity and released from drug complexes (P-CNCs-D) with pH dependent behavior.
34087297	2	36	attach	released	514:521	arg2	DOX					453:455	DOX	453:455	DOX	453:455	DOX could be loaded on PEGylated CNCs with high capacity and released from drug complexes (P-CNCs-D) with pH dependent behavior.
34087297	2	36	attach	released	514:521	arg1	P-CNCs-D					544:551	P-CNCs-D	544:551	P-CNCs-D	544:551	DOX could be loaded on PEGylated CNCs with high capacity and released from drug complexes (P-CNCs-D) with pH dependent behavior.
34087297	3	37	theme	anticancer	756:765	arg1	effects					767:773	desirable anticancer effects	746:773	desirable anticancer effects	746:773	The biological evaluation results demonstrated that drug carriers (CNCs-EBO-NH) showed negative cytotoxicity while DOX could be transported into cells and exhibits desirable anticancer effects.
34087297	4	38	theme	surface	913:919	arg1	functionalization					921:937	surface functionalization	913:937	surface functionalization	913:937	As compared with other method, the method developed in this work is rather simple and effective and can be achieved for simultaneous for surface functionalization and drug loading in a one-pot route.
34087297	5	39	theme	biomedical	1150:1159	arg1	fields					1161:1166	biomedical fields	1150:1166	biomedical fields	1150:1166	This work will open a new avenue for fabrication of various multifunctional composites based on other carbohydrate polymers or materials and to explore their applications in biomedical fields.
34087297	1	40	theme	aldehyde	330:337	arg1	group					339:343	aldehyde group	330:343	aldehyde group	330:343	Herein, a novel strategy for surface functionalization and drug loading of cellulose nanocrystals (CNCs) through formation of hydrazone bonds between functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX) was reported for the first time.
34087297	3	41	theme	negative	669:676	arg1	cytotoxicity					678:689	negative cytotoxicity	669:689	negative cytotoxicity	669:689	The biological evaluation results demonstrated that drug carriers (CNCs-EBO-NH) showed negative cytotoxicity while DOX could be transported into cells and exhibits desirable anticancer effects.
34087297	4	42	theme	other	793:797	arg1	method					799:804	other method	793:804	other method	793:804	As compared with other method, the method developed in this work is rather simple and effective and can be achieved for simultaneous for surface functionalization and drug loading in a one-pot route.
34087297	1	43	contain	containing	345:354	arg1	CNCs					321:324	functionalized CNCs	306:324	functionalized CNCs	306:324	Herein, a novel strategy for surface functionalization and drug loading of cellulose nanocrystals (CNCs) through formation of hydrazone bonds between functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX) was reported for the first time.
34087297	1	43	contain	containing	345:354	arg2	CHO-PEG					377:383	CHO-PEG	377:383	CHO-PEG	377:383	Herein, a novel strategy for surface functionalization and drug loading of cellulose nanocrystals (CNCs) through formation of hydrazone bonds between functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX) was reported for the first time.
34087297	1	43	contain	containing	345:354	arg2	glycol					369:374	polyethylene glycol	356:374	polyethylene glycol (CHO-PEG)	356:384	Herein, a novel strategy for surface functionalization and drug loading of cellulose nanocrystals (CNCs) through formation of hydrazone bonds between functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX) was reported for the first time.
34087297	5	44	theme	various	1028:1034	arg1	composites					1052:1061	various multifunctional composites	1028:1061	various multifunctional composites based on other carbohydrate polymers or materials	1028:1111	This work will open a new avenue for fabrication of various multifunctional composites based on other carbohydrate polymers or materials and to explore their applications in biomedical fields.
34087297	0	45	dep	functionalization	21:37	arg1	method					65:70	A novel method	57:70	Simultaneous surface functionalization and drug loading: A novel method for fabrication of cellulose nanocrystals-based pH responsive drug delivery system.	0:154	Simultaneous surface functionalization and drug loading: A novel method for fabrication of cellulose nanocrystals-based pH responsive drug delivery system.
34087297	1	46	theme	cellulose	231:239	arg1	nanocrystals					241:252	cellulose nanocrystals	231:252	cellulose nanocrystals (CNCs)	231:259	Herein, a novel strategy for surface functionalization and drug loading of cellulose nanocrystals (CNCs) through formation of hydrazone bonds between functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX) was reported for the first time.
34087297	1	46	theme	cellulose	231:239	arg1	CNCs					255:258	CNCs	255:258	CNCs	255:258	Herein, a novel strategy for surface functionalization and drug loading of cellulose nanocrystals (CNCs) through formation of hydrazone bonds between functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX) was reported for the first time.
34087297	4	47	theme	one-pot	961:967	arg1	route					969:973	a one-pot route	959:973	a one-pot route	959:973	As compared with other method, the method developed in this work is rather simple and effective and can be achieved for simultaneous for surface functionalization and drug loading in a one-pot route.
34087297	5	48	theme	multifunctional	1036:1050	arg1	composites					1052:1061	various multifunctional composites	1028:1061	various multifunctional composites based on other carbohydrate polymers or materials	1028:1111	This work will open a new avenue for fabrication of various multifunctional composites based on other carbohydrate polymers or materials and to explore their applications in biomedical fields.
34087297	3	49	theme	biological	586:595	arg1	evaluation					597:606	The biological evaluation	582:606	The biological evaluation	582:606	The biological evaluation results demonstrated that drug carriers (CNCs-EBO-NH) showed negative cytotoxicity while DOX could be transported into cells and exhibits desirable anticancer effects.
34087297	1	50	theme	nanocrystals	241:252	arg1	functionalization					193:209	surface functionalization	185:209	surface functionalization	185:209	Herein, a novel strategy for surface functionalization and drug loading of cellulose nanocrystals (CNCs) through formation of hydrazone bonds between functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX) was reported for the first time.
34087297	1	50	theme	nanocrystals	241:252	arg1	loading					220:226	drug loading	215:226	drug loading	215:226	Herein, a novel strategy for surface functionalization and drug loading of cellulose nanocrystals (CNCs) through formation of hydrazone bonds between functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX) was reported for the first time.
34087297	0	51	theme	novel	59:63	arg1	method					65:70	A novel method	57:70	Simultaneous surface functionalization and drug loading: A novel method for fabrication of cellulose nanocrystals-based pH responsive drug delivery system.	0:154	Simultaneous surface functionalization and drug loading: A novel method for fabrication of cellulose nanocrystals-based pH responsive drug delivery system.
34087297	1	52	theme	group	339:343	arg1	DOX					415:417	DOX	415:417	DOX	415:417	Herein, a novel strategy for surface functionalization and drug loading of cellulose nanocrystals (CNCs) through formation of hydrazone bonds between functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX) was reported for the first time.
34087297	1	52	theme	group	339:343	arg1	doxorubicin					402:412	functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin	306:412	functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX)	306:418	Herein, a novel strategy for surface functionalization and drug loading of cellulose nanocrystals (CNCs) through formation of hydrazone bonds between functionalized CNCs and aldehyde group containing polyethylene glycol (CHO-PEG)/anticancer drug doxorubicin (DOX) was reported for the first time.
32396166	4	0	from	immunotherapy	604:616	arg1	mice					621:624	mice	621:624	mice	621:624	To better understand how PitiumVac® works, we analyzed the composition of PitiumVac® and the immune response triggered by this immunotherapy in mice.
32396166	7	1	theme	cytokine	1088:1095	arg1	levels					1097:1102	The highest cytokine levels	1076:1102	The highest cytokine levels	1076:1102	The highest cytokine levels were observed after the third immunization, principally for IFN-γ, IL-17A, IL-6, and IL-10 levels.
32396166	5	2	theme	enzymatic	643:651	arg1	quantification					653:666	an enzymatic quantification	640:666	an enzymatic quantification that showed a total glucan content of 21.05% ± 0.94 (α-glucan, 6.37% ± 0.77 and (1,3)(1,6)-β-glucan, 14.68% ± 0.60) and mannose content of 1.39% ± 0.26	640:818	We performed an enzymatic quantification that showed a total glucan content of 21.05% ± 0.94 (α-glucan, 6.37% ± 0.77 and (1,3)(1,6)-β-glucan, 14.68% ± 0.60) and mannose content of 1.39% ± 0.26; the protein content was 0.52 mg ml-1 ± 0.07 mg ml-1.
32396166	5	3	theme	1.39	807:810	arg1	%					811:811	%	811:811	%	811:811	We performed an enzymatic quantification that showed a total glucan content of 21.05% ± 0.94 (α-glucan, 6.37% ± 0.77 and (1,3)(1,6)-β-glucan, 14.68% ± 0.60) and mannose content of 1.39% ± 0.26; the protein content was 0.52 mg ml-1 ± 0.07 mg ml-1.
32396166	4	4	theme	response	577:584	arg1	composition					536:546	the composition	532:546	the composition of PitiumVac® and the immune response triggered by this immunotherapy in mice	532:624	To better understand how PitiumVac® works, we analyzed the composition of PitiumVac® and the immune response triggered by this immunotherapy in mice.
32396166	8	5	theme	untreated	1367:1375	arg1	mice					1377:1380	untreated mice	1367:1380	untreated mice	1367:1380	Results of infected untreated (Pythiosis) and infected treated (Pythiosis + PVAC) mice (n = 3) showed that PitiumVac® reinforces the Th1/Th17 response displayed by untreated mice.
32396166	5	6	theme	%	811:811	arg1	0.26					815:818	1.39% ± 0.26	807:818	1.39% ± 0.26	807:818	We performed an enzymatic quantification that showed a total glucan content of 21.05% ± 0.94 (α-glucan, 6.37% ± 0.77 and (1,3)(1,6)-β-glucan, 14.68% ± 0.60) and mannose content of 1.39% ± 0.26; the protein content was 0.52 mg ml-1 ± 0.07 mg ml-1.
32396166	8	7	theme	Pythiosis	1234:1242	arg1	Results					1203:1209	Results	1203:1209	Results of infected untreated (Pythiosis) and infected treated (Pythiosis + PVAC) mice (n = 3)	1203:1296	Results of infected untreated (Pythiosis) and infected treated (Pythiosis + PVAC) mice (n = 3) showed that PitiumVac® reinforces the Th1/Th17 response displayed by untreated mice.
32396166	4	8	theme	immune	570:575	arg1	response					577:584	the immune response	566:584	the immune response triggered by this immunotherapy in mice	566:624	To better understand how PitiumVac® works, we analyzed the composition of PitiumVac® and the immune response triggered by this immunotherapy in mice.
32396166	3	9	theme	therapeutic	359:369	arg1	PitiumVac®					343:352	PitiumVac®	343:352	PitiumVac®	343:352	PitiumVac® is a therapeutic vaccine prepared from the mycelial mass of P. insidiosum and is used to treat Brazilian equine pythiosis.
32396166	3	9	theme	therapeutic	359:369	arg1	vaccine					371:377	a therapeutic vaccine	357:377	a therapeutic vaccine prepared from the mycelial mass of P. insidiosum	357:426	PitiumVac® is a therapeutic vaccine prepared from the mycelial mass of P. insidiosum and is used to treat Brazilian equine pythiosis.
32396166	8	10	theme	Pythiosis + PVAC	1267:1282	arg1	mice					1285:1288	infected untreated (Pythiosis) and infected treated (Pythiosis + PVAC) mice	1214:1288	mice	1285:1288	Results of infected untreated (Pythiosis) and infected treated (Pythiosis + PVAC) mice (n = 3) showed that PitiumVac® reinforces the Th1/Th17 response displayed by untreated mice.
32396166	9	11	theme	Th1/Th17	1457:1464	arg1	response					1466:1473	this Th1/Th17 response	1452:1473	this Th1/Th17 response	1452:1473	The (1,3)(1,6)-β-glucan content can be, at least in part, related to this Th1/Th17 response.
32396166	5	12	theme	ml-1 ± 0.07 mg	853:866	arg1	ml-1					868:871	0.52 mg ml-1 ± 0.07 mg ml-1	845:871	0.52 mg ml-1 ± 0.07 mg ml-1	845:871	We performed an enzymatic quantification that showed a total glucan content of 21.05% ± 0.94 (α-glucan, 6.37% ± 0.77 and (1,3)(1,6)-β-glucan, 14.68% ± 0.60) and mannose content of 1.39% ± 0.26; the protein content was 0.52 mg ml-1 ± 0.07 mg ml-1.
32396166	5	12	theme	ml-1 ± 0.07 mg	853:866	arg1	content					833:839	the protein content	821:839	the protein content	821:839	We performed an enzymatic quantification that showed a total glucan content of 21.05% ± 0.94 (α-glucan, 6.37% ± 0.77 and (1,3)(1,6)-β-glucan, 14.68% ± 0.60) and mannose content of 1.39% ± 0.26; the protein content was 0.52 mg ml-1 ± 0.07 mg ml-1.
32396166	6	13	theme	Th1	1016:1018	arg1	responses					1029:1037	a relevant Th1 and Th17 responses	1005:1037	a relevant Th1 and Th17 responses compared to nonimmunization of mice	1005:1073	Healthy Swiss mice (n = 3) were subcutaneously preimmunized with one, two, or three shots of PitiumVac®, and immunization promoted a relevant Th1 and Th17 responses compared to nonimmunization of mice.
32396166	5	14	theme	protein	825:831	arg1	ml-1					868:871	0.52 mg ml-1 ± 0.07 mg ml-1	845:871	0.52 mg ml-1 ± 0.07 mg ml-1	845:871	We performed an enzymatic quantification that showed a total glucan content of 21.05% ± 0.94 (α-glucan, 6.37% ± 0.77 and (1,3)(1,6)-β-glucan, 14.68% ± 0.60) and mannose content of 1.39% ± 0.26; the protein content was 0.52 mg ml-1 ± 0.07 mg ml-1.
32396166	5	14	theme	protein	825:831	arg1	content					833:839	the protein content	821:839	the protein content	821:839	We performed an enzymatic quantification that showed a total glucan content of 21.05% ± 0.94 (α-glucan, 6.37% ± 0.77 and (1,3)(1,6)-β-glucan, 14.68% ± 0.60) and mannose content of 1.39% ± 0.26; the protein content was 0.52 mg ml-1 ± 0.07 mg ml-1.
32396166	6	15	theme	PitiumVac®	967:976	arg1	shots					958:962	one, two, or three shots	939:962	one, two, or three shots of PitiumVac®	939:976	Healthy Swiss mice (n = 3) were subcutaneously preimmunized with one, two, or three shots of PitiumVac®, and immunization promoted a relevant Th1 and Th17 responses compared to nonimmunization of mice.
32396166	6	16	theme	Swiss	882:886	arg1	n = 3					894:898	n = 3	894:898	n = 3	894:898	Healthy Swiss mice (n = 3) were subcutaneously preimmunized with one, two, or three shots of PitiumVac®, and immunization promoted a relevant Th1 and Th17 responses compared to nonimmunization of mice.
32396166	6	16	theme	Swiss	882:886	arg1	mice					888:891	Healthy Swiss mice	874:891	Healthy Swiss mice (n = 3)	874:899	Healthy Swiss mice (n = 3) were subcutaneously preimmunized with one, two, or three shots of PitiumVac®, and immunization promoted a relevant Th1 and Th17 responses compared to nonimmunization of mice.
32396166	6	17	theme	relevant	1007:1014	arg1	Th1					1016:1018	a relevant Th1	1005:1018	a relevant Th1	1005:1018	Healthy Swiss mice (n = 3) were subcutaneously preimmunized with one, two, or three shots of PitiumVac®, and immunization promoted a relevant Th1 and Th17 responses compared to nonimmunization of mice.
32396166	8	18	theme	infected	1214:1221	arg1	n = 3					1291:1295	n = 3	1291:1295	n = 3	1291:1295	Results of infected untreated (Pythiosis) and infected treated (Pythiosis + PVAC) mice (n = 3) showed that PitiumVac® reinforces the Th1/Th17 response displayed by untreated mice.
32396166	8	18	theme	infected	1214:1221	arg1	Pythiosis					1234:1242	infected untreated (Pythiosis) and infected treated (Pythiosis + PVAC) mice	1214:1288	Pythiosis	1234:1242	Results of infected untreated (Pythiosis) and infected treated (Pythiosis + PVAC) mice (n = 3) showed that PitiumVac® reinforces the Th1/Th17 response displayed by untreated mice.
32396166	9	19	from	related	1441:1447	arg1	part					1435:1438	part	1435:1438	part	1435:1438	The (1,3)(1,6)-β-glucan content can be, at least in part, related to this Th1/Th17 response.
32396166	9	20	theme	1,6	1393:1395	arg1	content					1407:1413	The (1,3)(1,6)-β-glucan content	1383:1413	The (1,3)(1,6)-β-glucan content	1383:1413	The (1,3)(1,6)-β-glucan content can be, at least in part, related to this Th1/Th17 response.
32396166	9	20	theme	1,6	1393:1395	arg1	related					1441:1447	related	1441:1447	related	1441:1447	The (1,3)(1,6)-β-glucan content can be, at least in part, related to this Th1/Th17 response.
32396166	3	21	used	used	435:438	arg2	vaccine					371:377	a therapeutic vaccine	357:377	a therapeutic vaccine prepared from the mycelial mass of P. insidiosum	357:426	PitiumVac® is a therapeutic vaccine prepared from the mycelial mass of P. insidiosum and is used to treat Brazilian equine pythiosis.
32396166	3	21	used	used	435:438	arg2	PitiumVac®					343:352	PitiumVac®	343:352	PitiumVac®	343:352	PitiumVac® is a therapeutic vaccine prepared from the mycelial mass of P. insidiosum and is used to treat Brazilian equine pythiosis.
32396166	6	22	theme	Healthy	874:880	arg1	n = 3					894:898	n = 3	894:898	n = 3	894:898	Healthy Swiss mice (n = 3) were subcutaneously preimmunized with one, two, or three shots of PitiumVac®, and immunization promoted a relevant Th1 and Th17 responses compared to nonimmunization of mice.
32396166	6	22	theme	Healthy	874:880	arg1	mice					888:891	Healthy Swiss mice	874:891	Healthy Swiss mice (n = 3)	874:899	Healthy Swiss mice (n = 3) were subcutaneously preimmunized with one, two, or three shots of PitiumVac®, and immunization promoted a relevant Th1 and Th17 responses compared to nonimmunization of mice.
32396166	2	23	theme	preferred	314:322	arg1	interventions					230:242	surgical interventions	221:242	surgical interventions associated with antimicrobial therapy, immunotherapy, or both	221:304	Typically, surgical interventions associated with antimicrobial therapy, immunotherapy, or both are the preferred treatment choices.
32396166	2	23	theme	preferred	314:322	arg1	choices					334:340	the preferred treatment choices	310:340	the preferred treatment choices	310:340	Typically, surgical interventions associated with antimicrobial therapy, immunotherapy, or both are the preferred treatment choices.
32396166	9	24	from	part	1435:1438	arg1	content					1407:1413	The (1,3)(1,6)-β-glucan content	1383:1413	The (1,3)(1,6)-β-glucan content	1383:1413	The (1,3)(1,6)-β-glucan content can be, at least in part, related to this Th1/Th17 response.
32396166	9	24	from	part	1435:1438	arg1	related					1441:1447	related	1441:1447	related	1441:1447	The (1,3)(1,6)-β-glucan content can be, at least in part, related to this Th1/Th17 response.
32396166	3	25	theme	mycelial	397:404	arg1	mass					406:409	the mycelial mass	393:409	the mycelial mass of P. insidiosum	393:426	PitiumVac® is a therapeutic vaccine prepared from the mycelial mass of P. insidiosum and is used to treat Brazilian equine pythiosis.
32396166	4	26	theme	PitiumVac®	551:560	arg1	composition					536:546	the composition	532:546	the composition of PitiumVac® and the immune response triggered by this immunotherapy in mice	532:624	To better understand how PitiumVac® works, we analyzed the composition of PitiumVac® and the immune response triggered by this immunotherapy in mice.
32396166	1	27	theme	Pythium	86:92	arg1	insidiosum					94:103	Pythium insidiosum	86:103	Pythium insidiosum	86:103	Pythium insidiosum is an oomycete that affects mammals, especially humans and horses, causing a difficult-to-treat disease.
32396166	1	27	theme	Pythium	86:92	arg1	oomycete					111:118	an oomycete	108:118	an oomycete that affects mammals, especially humans and horses, causing a difficult-to-treat disease	108:207	Pythium insidiosum is an oomycete that affects mammals, especially humans and horses, causing a difficult-to-treat disease.
32396166	5	28	theme	mannose	788:794	arg1	content					796:802	mannose content	788:802	mannose content of 1.39% ± 0.26	788:818	We performed an enzymatic quantification that showed a total glucan content of 21.05% ± 0.94 (α-glucan, 6.37% ± 0.77 and (1,3)(1,6)-β-glucan, 14.68% ± 0.60) and mannose content of 1.39% ± 0.26; the protein content was 0.52 mg ml-1 ± 0.07 mg ml-1.
32396166	5	29	theme	1,6	754:756	arg1	-β-glucan					758:766	(1,3)(1,6)-β-glucan	748:766	(1,3)(1,6)-β-glucan	748:766	We performed an enzymatic quantification that showed a total glucan content of 21.05% ± 0.94 (α-glucan, 6.37% ± 0.77 and (1,3)(1,6)-β-glucan, 14.68% ± 0.60) and mannose content of 1.39% ± 0.26; the protein content was 0.52 mg ml-1 ± 0.07 mg ml-1.
32396166	3	30	theme	equine	459:464	arg1	pythiosis					466:474	Brazilian equine pythiosis	449:474	Brazilian equine pythiosis	449:474	PitiumVac® is a therapeutic vaccine prepared from the mycelial mass of P. insidiosum and is used to treat Brazilian equine pythiosis.
32396166	6	31	theme	Th17	1024:1027	arg1	responses					1029:1037	a relevant Th1 and Th17 responses	1005:1037	a relevant Th1 and Th17 responses compared to nonimmunization of mice	1005:1073	Healthy Swiss mice (n = 3) were subcutaneously preimmunized with one, two, or three shots of PitiumVac®, and immunization promoted a relevant Th1 and Th17 responses compared to nonimmunization of mice.
32396166	5	32	theme	0.52 mg	845:851	arg1	ml-1					868:871	0.52 mg ml-1 ± 0.07 mg ml-1	845:871	0.52 mg ml-1 ± 0.07 mg ml-1	845:871	We performed an enzymatic quantification that showed a total glucan content of 21.05% ± 0.94 (α-glucan, 6.37% ± 0.77 and (1,3)(1,6)-β-glucan, 14.68% ± 0.60) and mannose content of 1.39% ± 0.26; the protein content was 0.52 mg ml-1 ± 0.07 mg ml-1.
32396166	5	32	theme	0.52 mg	845:851	arg1	content					833:839	the protein content	821:839	the protein content	821:839	We performed an enzymatic quantification that showed a total glucan content of 21.05% ± 0.94 (α-glucan, 6.37% ± 0.77 and (1,3)(1,6)-β-glucan, 14.68% ± 0.60) and mannose content of 1.39% ± 0.26; the protein content was 0.52 mg ml-1 ± 0.07 mg ml-1.
32396166	1	33	theme	difficult-to-treat	182:199	arg1	disease					201:207	a difficult-to-treat disease	180:207	a difficult-to-treat disease	180:207	Pythium insidiosum is an oomycete that affects mammals, especially humans and horses, causing a difficult-to-treat disease.
32396166	5	34	theme	%	711:711	arg1	content					695:701	a total glucan content	680:701	a total glucan content of 21.05%	680:711	We performed an enzymatic quantification that showed a total glucan content of 21.05% ± 0.94 (α-glucan, 6.37% ± 0.77 and (1,3)(1,6)-β-glucan, 14.68% ± 0.60) and mannose content of 1.39% ± 0.26; the protein content was 0.52 mg ml-1 ± 0.07 mg ml-1.
32396166	8	35	theme	infected	1249:1256	arg1	mice					1285:1288	infected untreated (Pythiosis) and infected treated (Pythiosis + PVAC) mice	1214:1288	mice	1285:1288	Results of infected untreated (Pythiosis) and infected treated (Pythiosis + PVAC) mice (n = 3) showed that PitiumVac® reinforces the Th1/Th17 response displayed by untreated mice.
32396166	3	36	theme	insidiosum	417:426	arg1	mass					406:409	the mycelial mass	393:409	the mycelial mass of P. insidiosum	393:426	PitiumVac® is a therapeutic vaccine prepared from the mycelial mass of P. insidiosum and is used to treat Brazilian equine pythiosis.
32396166	5	37	dep	%	735:735	arg1	-β-glucan					758:766	(1,3)(1,6)-β-glucan	748:766	(1,3)(1,6)-β-glucan	748:766	We performed an enzymatic quantification that showed a total glucan content of 21.05% ± 0.94 (α-glucan, 6.37% ± 0.77 and (1,3)(1,6)-β-glucan, 14.68% ± 0.60) and mannose content of 1.39% ± 0.26; the protein content was 0.52 mg ml-1 ± 0.07 mg ml-1.
32396166	5	37	dep	%	735:735	arg1	0.77					739:742	0.77	739:742	0.77	739:742	We performed an enzymatic quantification that showed a total glucan content of 21.05% ± 0.94 (α-glucan, 6.37% ± 0.77 and (1,3)(1,6)-β-glucan, 14.68% ± 0.60) and mannose content of 1.39% ± 0.26; the protein content was 0.52 mg ml-1 ± 0.07 mg ml-1.
32396166	0	38	from	response	68:75	arg1	mice					80:83	mice	80:83	mice	80:83	Immunotherapy based on Pythium insidiosum mycelia drives a Th1/Th17 response in mice.
32396166	2	39	theme	surgical	221:228	arg1	interventions					230:242	surgical interventions	221:242	surgical interventions associated with antimicrobial therapy, immunotherapy, or both	221:304	Typically, surgical interventions associated with antimicrobial therapy, immunotherapy, or both are the preferred treatment choices.
32396166	2	39	theme	surgical	221:228	arg1	choices					334:340	the preferred treatment choices	310:340	the preferred treatment choices	310:340	Typically, surgical interventions associated with antimicrobial therapy, immunotherapy, or both are the preferred treatment choices.
32396166	3	40	theme	Brazilian	449:457	arg1	pythiosis					466:474	Brazilian equine pythiosis	449:474	Brazilian equine pythiosis	449:474	PitiumVac® is a therapeutic vaccine prepared from the mycelial mass of P. insidiosum and is used to treat Brazilian equine pythiosis.
32396166	5	41	theme	1,3	749:751	arg1	-β-glucan					758:766	(1,3)(1,6)-β-glucan	748:766	(1,3)(1,6)-β-glucan	748:766	We performed an enzymatic quantification that showed a total glucan content of 21.05% ± 0.94 (α-glucan, 6.37% ± 0.77 and (1,3)(1,6)-β-glucan, 14.68% ± 0.60) and mannose content of 1.39% ± 0.26; the protein content was 0.52 mg ml-1 ± 0.07 mg ml-1.
32396166	5	42	dep	α-glucan	721:728	arg1	%					774:774	14.68% ± 0.60	769:781	14.68% ± 0.60	769:781	We performed an enzymatic quantification that showed a total glucan content of 21.05% ± 0.94 (α-glucan, 6.37% ± 0.77 and (1,3)(1,6)-β-glucan, 14.68% ± 0.60) and mannose content of 1.39% ± 0.26; the protein content was 0.52 mg ml-1 ± 0.07 mg ml-1.
32396166	5	42	dep	α-glucan	721:728	arg1	%					735:735	6.37% ±	731:737	6.37% ± 0.77 and (1,3)(1,6)-β-glucan	731:766	We performed an enzymatic quantification that showed a total glucan content of 21.05% ± 0.94 (α-glucan, 6.37% ± 0.77 and (1,3)(1,6)-β-glucan, 14.68% ± 0.60) and mannose content of 1.39% ± 0.26; the protein content was 0.52 mg ml-1 ± 0.07 mg ml-1.
32396166	6	43	with	preimmunized	921:932	arg1	shots					958:962	one, two, or three shots	939:962	one, two, or three shots of PitiumVac®	939:976	Healthy Swiss mice (n = 3) were subcutaneously preimmunized with one, two, or three shots of PitiumVac®, and immunization promoted a relevant Th1 and Th17 responses compared to nonimmunization of mice.
32396166	5	44	dep	0.94	715:718	arg1	α-glucan					721:728	α-glucan	721:728	α-glucan	721:728	We performed an enzymatic quantification that showed a total glucan content of 21.05% ± 0.94 (α-glucan, 6.37% ± 0.77 and (1,3)(1,6)-β-glucan, 14.68% ± 0.60) and mannose content of 1.39% ± 0.26; the protein content was 0.52 mg ml-1 ± 0.07 mg ml-1.
32396166	5	45	theme	±	813:813	arg1	0.26					815:818	1.39% ± 0.26	807:818	1.39% ± 0.26	807:818	We performed an enzymatic quantification that showed a total glucan content of 21.05% ± 0.94 (α-glucan, 6.37% ± 0.77 and (1,3)(1,6)-β-glucan, 14.68% ± 0.60) and mannose content of 1.39% ± 0.26; the protein content was 0.52 mg ml-1 ± 0.07 mg ml-1.
32396166	5	46	theme	total	682:686	arg1	content					695:701	a total glucan content	680:701	a total glucan content of 21.05%	680:711	We performed an enzymatic quantification that showed a total glucan content of 21.05% ± 0.94 (α-glucan, 6.37% ± 0.77 and (1,3)(1,6)-β-glucan, 14.68% ± 0.60) and mannose content of 1.39% ± 0.26; the protein content was 0.52 mg ml-1 ± 0.07 mg ml-1.
32396166	9	47	theme	1,3	1388:1390	arg1	content					1407:1413	The (1,3)(1,6)-β-glucan content	1383:1413	The (1,3)(1,6)-β-glucan content	1383:1413	The (1,3)(1,6)-β-glucan content can be, at least in part, related to this Th1/Th17 response.
32396166	9	47	theme	1,3	1388:1390	arg1	related					1441:1447	related	1441:1447	related	1441:1447	The (1,3)(1,6)-β-glucan content can be, at least in part, related to this Th1/Th17 response.
32396166	8	48	theme	treated	1258:1264	arg1	mice					1285:1288	infected untreated (Pythiosis) and infected treated (Pythiosis + PVAC) mice	1214:1288	mice	1285:1288	Results of infected untreated (Pythiosis) and infected treated (Pythiosis + PVAC) mice (n = 3) showed that PitiumVac® reinforces the Th1/Th17 response displayed by untreated mice.
32396166	5	49	theme	0.26	815:818	arg1	content					796:802	mannose content	788:802	mannose content of 1.39% ± 0.26	788:818	We performed an enzymatic quantification that showed a total glucan content of 21.05% ± 0.94 (α-glucan, 6.37% ± 0.77 and (1,3)(1,6)-β-glucan, 14.68% ± 0.60) and mannose content of 1.39% ± 0.26; the protein content was 0.52 mg ml-1 ± 0.07 mg ml-1.
32396166	8	50	theme	mice	1285:1288	arg1	Results					1203:1209	Results	1203:1209	Results of infected untreated (Pythiosis) and infected treated (Pythiosis + PVAC) mice (n = 3)	1203:1296	Results of infected untreated (Pythiosis) and infected treated (Pythiosis + PVAC) mice (n = 3) showed that PitiumVac® reinforces the Th1/Th17 response displayed by untreated mice.
32396166	5	51	theme	glucan	688:693	arg1	content					695:701	a total glucan content	680:701	a total glucan content of 21.05%	680:711	We performed an enzymatic quantification that showed a total glucan content of 21.05% ± 0.94 (α-glucan, 6.37% ± 0.77 and (1,3)(1,6)-β-glucan, 14.68% ± 0.60) and mannose content of 1.39% ± 0.26; the protein content was 0.52 mg ml-1 ± 0.07 mg ml-1.
32396166	8	52	theme	untreated	1223:1231	arg1	n = 3					1291:1295	n = 3	1291:1295	n = 3	1291:1295	Results of infected untreated (Pythiosis) and infected treated (Pythiosis + PVAC) mice (n = 3) showed that PitiumVac® reinforces the Th1/Th17 response displayed by untreated mice.
32396166	8	52	theme	untreated	1223:1231	arg1	Pythiosis					1234:1242	infected untreated (Pythiosis) and infected treated (Pythiosis + PVAC) mice	1214:1288	Pythiosis	1234:1242	Results of infected untreated (Pythiosis) and infected treated (Pythiosis + PVAC) mice (n = 3) showed that PitiumVac® reinforces the Th1/Th17 response displayed by untreated mice.
32396166	9	53	theme	-β-glucan	1397:1405	arg1	content					1407:1413	The (1,3)(1,6)-β-glucan content	1383:1413	The (1,3)(1,6)-β-glucan content	1383:1413	The (1,3)(1,6)-β-glucan content can be, at least in part, related to this Th1/Th17 response.
32396166	9	53	theme	-β-glucan	1397:1405	arg1	related					1441:1447	related	1441:1447	related	1441:1447	The (1,3)(1,6)-β-glucan content can be, at least in part, related to this Th1/Th17 response.
32396166	7	54	dep	IFN-γ	1164:1168	arg1	levels					1195:1200	levels	1195:1200	levels	1195:1200	The highest cytokine levels were observed after the third immunization, principally for IFN-γ, IL-17A, IL-6, and IL-10 levels.
32396166	8	55	theme	Th1/Th17	1336:1343	arg1	response					1345:1352	the Th1/Th17 response	1332:1352	the Th1/Th17 response displayed by untreated mice	1332:1380	Results of infected untreated (Pythiosis) and infected treated (Pythiosis + PVAC) mice (n = 3) showed that PitiumVac® reinforces the Th1/Th17 response displayed by untreated mice.
32396166	2	56	theme	antimicrobial	260:272	arg1	therapy					274:280	antimicrobial therapy	260:280	antimicrobial therapy	260:280	Typically, surgical interventions associated with antimicrobial therapy, immunotherapy, or both are the preferred treatment choices.
32396166	2	57	theme	treatment	324:332	arg1	interventions					230:242	surgical interventions	221:242	surgical interventions associated with antimicrobial therapy, immunotherapy, or both	221:304	Typically, surgical interventions associated with antimicrobial therapy, immunotherapy, or both are the preferred treatment choices.
32396166	2	57	theme	treatment	324:332	arg1	choices					334:340	the preferred treatment choices	310:340	the preferred treatment choices	310:340	Typically, surgical interventions associated with antimicrobial therapy, immunotherapy, or both are the preferred treatment choices.
32396166	5	58	dep	showed	673:678	arg1	±					713:713	±	713:713	showed a total glucan content of 21.05% ± 0.94 (α-glucan, 6.37% ± 0.77 and (1,3)(1,6)-β-glucan, 14.68% ± 0.60) and mannose content of 1.39% ± 0.26	673:818	We performed an enzymatic quantification that showed a total glucan content of 21.05% ± 0.94 (α-glucan, 6.37% ± 0.77 and (1,3)(1,6)-β-glucan, 14.68% ± 0.60) and mannose content of 1.39% ± 0.26; the protein content was 0.52 mg ml-1 ± 0.07 mg ml-1.
32396166	7	59	theme	third	1128:1132	arg1	immunization					1134:1145	the third immunization	1124:1145	the third immunization	1124:1145	The highest cytokine levels were observed after the third immunization, principally for IFN-γ, IL-17A, IL-6, and IL-10 levels.
32396166	0	60	theme	Th1/Th17	59:66	arg1	response					68:75	a Th1/Th17 response	57:75	a Th1/Th17 response in mice	57:83	Immunotherapy based on Pythium insidiosum mycelia drives a Th1/Th17 response in mice.
32396166	6	61	theme	mice	1070:1073	arg1	nonimmunization					1051:1065	nonimmunization	1051:1065	nonimmunization of mice	1051:1073	Healthy Swiss mice (n = 3) were subcutaneously preimmunized with one, two, or three shots of PitiumVac®, and immunization promoted a relevant Th1 and Th17 responses compared to nonimmunization of mice.
32396166	7	62	theme	highest	1080:1086	arg1	levels					1097:1102	The highest cytokine levels	1076:1102	The highest cytokine levels	1076:1102	The highest cytokine levels were observed after the third immunization, principally for IFN-γ, IL-17A, IL-6, and IL-10 levels.
32930817	0	0	theme	tissue	27:32	arg1	preparation					7:17	Sample preparation	0:17	Sample preparation of bone tissue for MALDI-MSI for forensic and (pre)clinical applications.	0:91	Sample preparation of bone tissue for MALDI-MSI for forensic and (pre)clinical applications.
32930817	3	1	from	interface	633:641	arg1	particular					646:655	particular	646:655	particular	646:655	Undecalcified sections are preferred to retain sample integrity as much as possible or to study the tissue-bone bio interface in particular.
32930817	13	2	theme	unknown	1821:1827	arg1	lipids					1829:1834	unknown lipids	1821:1834	unknown lipids	1821:1834	The best matrices for the untargeted detection of unknown lipids in mouse hind legs in positive ion mode were CHCA and DHB based on the number of tissue-specific peaks and signal-to-noise ratios.
32930817	1	3	theme	samples	244:250	arg1	range					224:228	a broad range	216:228	a broad range of biological samples, e.g., forensics and preclinical samples	216:291	In the past decades, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been applied to a broad range of biological samples, e.g., forensics and preclinical samples.
32930817	10	4	from	detection	1499:1507	arg1	mode					1547:1550	positive ion mode	1534:1550	positive ion mode	1534:1550	DHB proved to be the most suitable matrix for the detection of methadone and EDDP in positive ion mode.
32930817	7	5	theme	longitudinal	1117:1128	arg1	fashion					1130:1136	a longitudinal fashion	1115:1136	a longitudinal fashion	1115:1136	Bone tissue has to be sectioned with a tungsten carbide knife in a longitudinal fashion, while the sections need to be supported with double-sided tapes to maintain the morphology of the tissue.
32930817	1	6	theme	spectrometry	163:174	arg1	imaging					176:182	matrix-assisted laser desorption/ionization mass spectrometry imaging	114:182	matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI)	114:194	In the past decades, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been applied to a broad range of biological samples, e.g., forensics and preclinical samples.
32930817	1	6	theme	spectrometry	163:174	arg1	MALDI-MSI					185:193	MALDI-MSI	185:193	MALDI-MSI	185:193	In the past decades, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been applied to a broad range of biological samples, e.g., forensics and preclinical samples.
32930817	5	7	theme	Different	848:856	arg1	materials					943:951	embedding materials	933:951	embedding materials	933:951	Different concentrations of gelatin and carboxymethyl cellulose (CMC) were tested as embedding materials.
32930817	5	7	theme	Different	848:856	arg1	concentrations					858:871	Different concentrations	848:871	Different concentrations of gelatin and carboxymethyl cellulose (CMC)	848:916	Different concentrations of gelatin and carboxymethyl cellulose (CMC) were tested as embedding materials.
32930817	10	8	theme	suitable	1475:1482	arg1	DHB					1449:1451	DHB	1449:1451	DHB	1449:1451	DHB proved to be the most suitable matrix for the detection of methadone and EDDP in positive ion mode.
32930817	10	8	theme	suitable	1475:1482	arg1	matrix					1484:1489	the most suitable matrix	1466:1489	the most suitable matrix for the detection of methadone and EDDP in positive ion mode	1466:1550	DHB proved to be the most suitable matrix for the detection of methadone and EDDP in positive ion mode.
32930817	12	9	attach	presence	1691:1698	arg2	methadone					1703:1711	methadone	1703:1711	methadone	1703:1711	The protocol was successfully applied to detect the presence of methadone and EDDP in a dosed rat femur and a dosed human clavicle.
32930817	12	9	attach	presence	1691:1698	arg2	EDDP					1717:1720	EDDP	1717:1720	EDDP	1717:1720	The protocol was successfully applied to detect the presence of methadone and EDDP in a dosed rat femur and a dosed human clavicle.
32930817	12	9	attach	presence	1691:1698	arg1	clavicle					1761:1768	a dosed human clavicle	1747:1768	a dosed human clavicle	1747:1768	The protocol was successfully applied to detect the presence of methadone and EDDP in a dosed rat femur and a dosed human clavicle.
32930817	12	9	attach	presence	1691:1698	arg1	femur					1737:1741	a dosed rat femur	1725:1741	a dosed rat femur	1725:1741	The protocol was successfully applied to detect the presence of methadone and EDDP in a dosed rat femur and a dosed human clavicle.
32930817	13	10	theme	signal-to-noise	1943:1957	arg1	ratios					1959:1964	signal-to-noise ratios	1943:1964	signal-to-noise ratios	1943:1964	The best matrices for the untargeted detection of unknown lipids in mouse hind legs in positive ion mode were CHCA and DHB based on the number of tissue-specific peaks and signal-to-noise ratios.
32930817	3	11	theme	Undecalcified	517:529	arg1	sections					531:538	Undecalcified sections	517:538	Undecalcified sections	517:538	Undecalcified sections are preferred to retain sample integrity as much as possible or to study the tissue-bone bio interface in particular.
32930817	5	12	theme	cellulose	902:910	arg1	materials					943:951	embedding materials	933:951	embedding materials	933:951	Different concentrations of gelatin and carboxymethyl cellulose (CMC) were tested as embedding materials.
32930817	5	12	theme	cellulose	902:910	arg1	concentrations					858:871	Different concentrations	848:871	Different concentrations of gelatin and carboxymethyl cellulose (CMC)	848:916	Different concentrations of gelatin and carboxymethyl cellulose (CMC) were tested as embedding materials.
32930817	2	13	theme	undecalcified	490:502	arg1	tissue					509:514	undecalcified bone tissue	490:514	undecalcified bone tissue	490:514	The use of MALDI-MSI for the analysis of bone tissue has been limited due to the insulating properties of the material but more importantly the absence of a proper sample preparation protocol for undecalcified bone tissue.
32930817	2	14	theme	bone	335:338	arg1	tissue					340:345	bone tissue	335:345	bone tissue	335:345	The use of MALDI-MSI for the analysis of bone tissue has been limited due to the insulating properties of the material but more importantly the absence of a proper sample preparation protocol for undecalcified bone tissue.
32930817	10	15	theme	methadone	1512:1520	arg1	detection					1499:1507	the detection	1495:1507	the detection of methadone and EDDP in positive ion mode	1495:1550	DHB proved to be the most suitable matrix for the detection of methadone and EDDP in positive ion mode.
32930817	3	16	theme	sample	564:569	arg1	integrity					571:579	sample integrity	564:579	sample integrity	564:579	Undecalcified sections are preferred to retain sample integrity as much as possible or to study the tissue-bone bio interface in particular.
32930817	14	17	theme	developed	1971:1979	arg1	method					2014:2019	The developed and optimized sample preparation method	1967:2019	The developed and optimized sample preparation method	1967:2019	The developed and optimized sample preparation method, applicable on animal and human bones, opens the door for future forensic and (pre)clinical investigations.
32930817	9	18	theme	untargeted	1393:1402	arg1	detection					1421:1429	Targeted (methadone and EDDP) as well as untargeted (unknown lipids) detection	1352:1429	Targeted (methadone and EDDP) as well as untargeted (unknown lipids) detection	1352:1429	Targeted (methadone and EDDP) as well as untargeted (unknown lipids) detection was demonstrated.
32930817	13	19	theme	peaks	1933:1937	arg1	number					1907:1912	the number	1903:1912	the number of tissue-specific peaks and signal-to-noise ratios	1903:1964	The best matrices for the untargeted detection of unknown lipids in mouse hind legs in positive ion mode were CHCA and DHB based on the number of tissue-specific peaks and signal-to-noise ratios.
32930817	9	20	theme	unknown	1405:1411	arg1	lipids					1413:1418	unknown lipids	1405:1418	unknown lipids	1405:1418	Targeted (methadone and EDDP) as well as untargeted (unknown lipids) detection was demonstrated.
32930817	6	21	theme	7.5	989:991	arg1	%					992:992	%	992:992	%	992:992	The composition of 20% gelatin and 7.5% CMC showed to support the tissue best while sectioning.
32930817	8	22	theme	developed	1249:1257	arg1	applicable					1293:1302	applicable	1293:1302	applicable	1293:1302	The developed sectioning method was shown to be applicable on rat and mouse as well as human bone samples.
32930817	8	22	theme	developed	1249:1257	arg1	method					1270:1275	The developed sectioning method	1245:1275	The developed sectioning method	1245:1275	The developed sectioning method was shown to be applicable on rat and mouse as well as human bone samples.
32930817	13	23	theme	best	1775:1778	arg1	matrices					1780:1787	The best matrices	1771:1787	The best matrices for the untargeted detection of unknown lipids in mouse hind legs in positive ion mode	1771:1874	The best matrices for the untargeted detection of unknown lipids in mouse hind legs in positive ion mode were CHCA and DHB based on the number of tissue-specific peaks and signal-to-noise ratios.
32930817	13	23	theme	best	1775:1778	arg1	CHCA					1881:1884	CHCA	1881:1884	CHCA	1881:1884	The best matrices for the untargeted detection of unknown lipids in mouse hind legs in positive ion mode were CHCA and DHB based on the number of tissue-specific peaks and signal-to-noise ratios.
32930817	0	24	theme	forensic	52:59	arg1	applications					79:90	forensic and (pre)clinical applications	52:90	forensic and (pre)clinical applications	52:90	Sample preparation of bone tissue for MALDI-MSI for forensic and (pre)clinical applications.
32930817	13	25	from	detection	1808:1816	arg1	legs					1850:1853	mouse hind legs	1839:1853	mouse hind legs in positive ion mode	1839:1874	The best matrices for the untargeted detection of unknown lipids in mouse hind legs in positive ion mode were CHCA and DHB based on the number of tissue-specific peaks and signal-to-noise ratios.
32930817	1	26	theme	matrix-assisted	114:128	arg1	spectrometry					163:174	matrix-assisted laser desorption/ionization mass spectrometry	114:174	matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI)	114:194	In the past decades, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been applied to a broad range of biological samples, e.g., forensics and preclinical samples.
32930817	6	27	theme	gelatin	977:983	arg1	composition					958:968	The composition	954:968	The composition of 20% gelatin and 7.5% CMC	954:996	The composition of 20% gelatin and 7.5% CMC showed to support the tissue best while sectioning.
32930817	1	28	theme	desorption/ionization	136:156	arg1	spectrometry					163:174	matrix-assisted laser desorption/ionization mass spectrometry	114:174	matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI)	114:194	In the past decades, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been applied to a broad range of biological samples, e.g., forensics and preclinical samples.
32930817	6	29	theme	20	973:974	arg1	%					975:975	%	975:975	%	975:975	The composition of 20% gelatin and 7.5% CMC showed to support the tissue best while sectioning.
32930817	2	30	theme	protocol	477:484	arg1	absence					438:444	the absence	434:444	the absence of a proper sample preparation protocol for undecalcified bone tissue	434:514	The use of MALDI-MSI for the analysis of bone tissue has been limited due to the insulating properties of the material but more importantly the absence of a proper sample preparation protocol for undecalcified bone tissue.
32930817	4	31	theme	bone	726:729	arg1	samples					731:737	undecalcified bone samples	712:737	undecalcified bone samples	712:737	Here, we optimized the sample preparation protocol of undecalcified bone samples, aimed at both targeted and untargeted applications for forensic and preclinical applications, respectively.
32930817	14	32	theme	clinical	2104:2111	arg1	investigations					2113:2126	future forensic and (pre)clinical investigations	2079:2126	future forensic and (pre)clinical investigations	2079:2126	The developed and optimized sample preparation method, applicable on animal and human bones, opens the door for future forensic and (pre)clinical investigations.
32930817	2	33	theme	sample	458:463	arg1	preparation					465:475	a proper sample preparation	449:475	a proper sample preparation protocol for undecalcified bone tissue	449:514	The use of MALDI-MSI for the analysis of bone tissue has been limited due to the insulating properties of the material but more importantly the absence of a proper sample preparation protocol for undecalcified bone tissue.
32930817	10	34	theme	ion	1543:1545	arg1	mode					1547:1550	positive ion mode	1534:1550	positive ion mode	1534:1550	DHB proved to be the most suitable matrix for the detection of methadone and EDDP in positive ion mode.
32930817	4	35	theme	preparation	688:698	arg1	protocol					700:707	the sample preparation protocol	677:707	the sample preparation protocol of undecalcified bone samples, aimed at both targeted and untargeted applications for forensic and preclinical applications	677:831	Here, we optimized the sample preparation protocol of undecalcified bone samples, aimed at both targeted and untargeted applications for forensic and preclinical applications, respectively.
32930817	2	36	theme	tissue	340:345	arg1	analysis					323:330	the analysis	319:330	the analysis of bone tissue	319:345	The use of MALDI-MSI for the analysis of bone tissue has been limited due to the insulating properties of the material but more importantly the absence of a proper sample preparation protocol for undecalcified bone tissue.
32930817	0	37	theme	Sample	0:5	arg1	preparation					7:17	Sample preparation	0:17	Sample preparation of bone tissue for MALDI-MSI for forensic and (pre)clinical applications.	0:91	Sample preparation of bone tissue for MALDI-MSI for forensic and (pre)clinical applications.
32930817	12	38	theme	dosed	1749:1753	arg1	clavicle					1761:1768	a dosed human clavicle	1747:1768	a dosed human clavicle	1747:1768	The protocol was successfully applied to detect the presence of methadone and EDDP in a dosed rat femur and a dosed human clavicle.
32930817	14	39	theme	future	2079:2084	arg1	investigations					2113:2126	future forensic and (pre)clinical investigations	2079:2126	future forensic and (pre)clinical investigations	2079:2126	The developed and optimized sample preparation method, applicable on animal and human bones, opens the door for future forensic and (pre)clinical investigations.
32930817	7	40	dep	tapes	1197:1201	arg1	maintain					1206:1213	maintain	1206:1213	to maintain the morphology of the tissue	1203:1242	Bone tissue has to be sectioned with a tungsten carbide knife in a longitudinal fashion, while the sections need to be supported with double-sided tapes to maintain the morphology of the tissue.
32930817	4	41	theme	untargeted	767:776	arg1	applications					778:789	both targeted and untargeted applications	749:789	both targeted and untargeted applications	749:789	Here, we optimized the sample preparation protocol of undecalcified bone samples, aimed at both targeted and untargeted applications for forensic and preclinical applications, respectively.
32930817	9	42	theme	Targeted	1352:1359	arg1	detection					1421:1429	Targeted (methadone and EDDP) as well as untargeted (unknown lipids) detection	1352:1429	Targeted (methadone and EDDP) as well as untargeted (unknown lipids) detection	1352:1429	Targeted (methadone and EDDP) as well as untargeted (unknown lipids) detection was demonstrated.
32930817	12	43	theme	rat	1733:1735	arg1	femur					1737:1741	a dosed rat femur	1725:1741	a dosed rat femur	1725:1741	The protocol was successfully applied to detect the presence of methadone and EDDP in a dosed rat femur and a dosed human clavicle.
32930817	6	44	theme	CMC	994:996	arg1	composition					958:968	The composition	954:968	The composition of 20% gelatin and 7.5% CMC	954:996	The composition of 20% gelatin and 7.5% CMC showed to support the tissue best while sectioning.
32930817	11	45	theme	detection	1566:1574	arg1	limit					1557:1561	The limit	1553:1561	The limit of detection (LOD)	1553:1580	The limit of detection (LOD) is estimated to approximately 50 pg/spot on bone tissue.
32930817	9	46	dep	Targeted	1352:1359	arg1	methadone					1362:1370	methadone	1362:1370	methadone	1362:1370	Targeted (methadone and EDDP) as well as untargeted (unknown lipids) detection was demonstrated.
32930817	9	46	dep	Targeted	1352:1359	arg1	lipids					1413:1418	unknown lipids	1405:1418	unknown lipids	1405:1418	Targeted (methadone and EDDP) as well as untargeted (unknown lipids) detection was demonstrated.
32930817	9	46	dep	Targeted	1352:1359	arg1	EDDP					1376:1379	EDDP	1376:1379	EDDP	1376:1379	Targeted (methadone and EDDP) as well as untargeted (unknown lipids) detection was demonstrated.
32930817	13	47	theme	positive	1858:1865	arg1	mode					1871:1874	positive ion mode	1858:1874	positive ion mode	1858:1874	The best matrices for the untargeted detection of unknown lipids in mouse hind legs in positive ion mode were CHCA and DHB based on the number of tissue-specific peaks and signal-to-noise ratios.
32930817	2	48	theme	MALDI-MSI	305:313	arg1	use					298:300	The use	294:300	The use of MALDI-MSI for the analysis of bone tissue	294:345	The use of MALDI-MSI for the analysis of bone tissue has been limited due to the insulating properties of the material but more importantly the absence of a proper sample preparation protocol for undecalcified bone tissue.
32930817	1	49	theme	samples	285:291	arg1	range					224:228	a broad range	216:228	a broad range of biological samples, e.g., forensics and preclinical samples	216:291	In the past decades, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been applied to a broad range of biological samples, e.g., forensics and preclinical samples.
32930817	12	50	theme	methadone	1703:1711	arg1	presence					1691:1698	the presence	1687:1698	the presence of methadone and EDDP in a dosed rat femur and a dosed human clavicle	1687:1768	The protocol was successfully applied to detect the presence of methadone and EDDP in a dosed rat femur and a dosed human clavicle.
32930817	12	51	from	presence	1691:1698	arg1	clavicle					1761:1768	a dosed human clavicle	1747:1768	a dosed human clavicle	1747:1768	The protocol was successfully applied to detect the presence of methadone and EDDP in a dosed rat femur and a dosed human clavicle.
32930817	12	51	from	presence	1691:1698	arg1	femur					1737:1741	a dosed rat femur	1725:1741	a dosed rat femur	1725:1741	The protocol was successfully applied to detect the presence of methadone and EDDP in a dosed rat femur and a dosed human clavicle.
32930817	8	52	theme	bone	1338:1341	arg1	samples					1343:1349	human bone samples	1332:1349	human bone samples	1332:1349	The developed sectioning method was shown to be applicable on rat and mouse as well as human bone samples.
32930817	12	53	theme	EDDP	1717:1720	arg1	presence					1691:1698	the presence	1687:1698	the presence of methadone and EDDP in a dosed rat femur and a dosed human clavicle	1687:1768	The protocol was successfully applied to detect the presence of methadone and EDDP in a dosed rat femur and a dosed human clavicle.
32930817	3	54	theme	tissue-bone	617:627	arg1	interface					633:641	the tissue-bone bio interface	613:641	the tissue-bone bio interface in particular	613:655	Undecalcified sections are preferred to retain sample integrity as much as possible or to study the tissue-bone bio interface in particular.
32930817	4	55	theme	targeted	754:761	arg1	applications					778:789	both targeted and untargeted applications	749:789	both targeted and untargeted applications	749:789	Here, we optimized the sample preparation protocol of undecalcified bone samples, aimed at both targeted and untargeted applications for forensic and preclinical applications, respectively.
32930817	13	56	theme	mouse	1839:1843	arg1	legs					1850:1853	mouse hind legs	1839:1853	mouse hind legs in positive ion mode	1839:1874	The best matrices for the untargeted detection of unknown lipids in mouse hind legs in positive ion mode were CHCA and DHB based on the number of tissue-specific peaks and signal-to-noise ratios.
32930817	13	57	from	legs	1850:1853	arg1	mode					1871:1874	positive ion mode	1858:1874	positive ion mode	1858:1874	The best matrices for the untargeted detection of unknown lipids in mouse hind legs in positive ion mode were CHCA and DHB based on the number of tissue-specific peaks and signal-to-noise ratios.
32930817	14	58	theme	human	2047:2051	arg1	bones					2053:2057	animal and human bones	2036:2057	animal and human bones	2036:2057	The developed and optimized sample preparation method, applicable on animal and human bones, opens the door for future forensic and (pre)clinical investigations.
32930817	1	59	theme	biological	233:242	arg1	samples					244:250	biological samples	233:250	biological samples	233:250	In the past decades, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been applied to a broad range of biological samples, e.g., forensics and preclinical samples.
32930817	5	60	theme	gelatin	876:882	arg1	materials					943:951	embedding materials	933:951	embedding materials	933:951	Different concentrations of gelatin and carboxymethyl cellulose (CMC) were tested as embedding materials.
32930817	5	60	theme	gelatin	876:882	arg1	concentrations					858:871	Different concentrations	848:871	Different concentrations of gelatin and carboxymethyl cellulose (CMC)	848:916	Different concentrations of gelatin and carboxymethyl cellulose (CMC) were tested as embedding materials.
32930817	14	61	theme	animal	2036:2041	arg1	bones					2053:2057	animal and human bones	2036:2057	animal and human bones	2036:2057	The developed and optimized sample preparation method, applicable on animal and human bones, opens the door for future forensic and (pre)clinical investigations.
32930817	7	62	theme	Bone	1050:1053	arg1	tissue					1055:1060	Bone tissue	1050:1060	Bone tissue	1050:1060	Bone tissue has to be sectioned with a tungsten carbide knife in a longitudinal fashion, while the sections need to be supported with double-sided tapes to maintain the morphology of the tissue.
32930817	13	63	theme	lipids	1829:1834	arg1	detection					1808:1816	the untargeted detection	1793:1816	the untargeted detection of unknown lipids in mouse hind legs in positive ion mode	1793:1874	The best matrices for the untargeted detection of unknown lipids in mouse hind legs in positive ion mode were CHCA and DHB based on the number of tissue-specific peaks and signal-to-noise ratios.
32930817	1	64	theme	mass	158:161	arg1	spectrometry					163:174	matrix-assisted laser desorption/ionization mass spectrometry	114:174	matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI)	114:194	In the past decades, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been applied to a broad range of biological samples, e.g., forensics and preclinical samples.
32930817	4	65	theme	forensic	795:802	arg1	applications					820:831	forensic and preclinical applications	795:831	forensic and preclinical applications	795:831	Here, we optimized the sample preparation protocol of undecalcified bone samples, aimed at both targeted and untargeted applications for forensic and preclinical applications, respectively.
32930817	14	66	theme	applicable	2022:2031	arg1	method					2014:2019	The developed and optimized sample preparation method	1967:2019	The developed and optimized sample preparation method	1967:2019	The developed and optimized sample preparation method, applicable on animal and human bones, opens the door for future forensic and (pre)clinical investigations.
32930817	7	67	theme	carbide	1098:1104	arg1	knife					1106:1110	a tungsten carbide knife	1087:1110	a tungsten carbide knife in a longitudinal fashion	1087:1136	Bone tissue has to be sectioned with a tungsten carbide knife in a longitudinal fashion, while the sections need to be supported with double-sided tapes to maintain the morphology of the tissue.
32930817	4	68	theme	preclinical	808:818	arg1	applications					820:831	forensic and preclinical applications	795:831	forensic and preclinical applications	795:831	Here, we optimized the sample preparation protocol of undecalcified bone samples, aimed at both targeted and untargeted applications for forensic and preclinical applications, respectively.
32930817	13	69	theme	untargeted	1797:1806	arg1	detection					1808:1816	the untargeted detection	1793:1816	the untargeted detection of unknown lipids in mouse hind legs in positive ion mode	1793:1874	The best matrices for the untargeted detection of unknown lipids in mouse hind legs in positive ion mode were CHCA and DHB based on the number of tissue-specific peaks and signal-to-noise ratios.
32930817	2	70	theme	bone	504:507	arg1	tissue					509:514	undecalcified bone tissue	490:514	undecalcified bone tissue	490:514	The use of MALDI-MSI for the analysis of bone tissue has been limited due to the insulating properties of the material but more importantly the absence of a proper sample preparation protocol for undecalcified bone tissue.
32930817	14	71	theme	sample	1995:2000	arg1	method					2014:2019	The developed and optimized sample preparation method	1967:2019	The developed and optimized sample preparation method	1967:2019	The developed and optimized sample preparation method, applicable on animal and human bones, opens the door for future forensic and (pre)clinical investigations.
32930817	13	72	theme	ratios	1959:1964	arg1	number					1907:1912	the number	1903:1912	the number of tissue-specific peaks and signal-to-noise ratios	1903:1964	The best matrices for the untargeted detection of unknown lipids in mouse hind legs in positive ion mode were CHCA and DHB based on the number of tissue-specific peaks and signal-to-noise ratios.
32930817	5	73	theme	carboxymethyl	888:900	arg1	cellulose					902:910	carboxymethyl cellulose	888:910	carboxymethyl cellulose (CMC)	888:916	Different concentrations of gelatin and carboxymethyl cellulose (CMC) were tested as embedding materials.
32930817	5	73	theme	carboxymethyl	888:900	arg1	CMC					913:915	CMC	913:915	CMC	913:915	Different concentrations of gelatin and carboxymethyl cellulose (CMC) were tested as embedding materials.
32930817	11	74	from	50 pg/spot	1612:1621	arg1	tissue					1631:1636	bone tissue	1626:1636	bone tissue	1626:1636	The limit of detection (LOD) is estimated to approximately 50 pg/spot on bone tissue.
32930817	13	75	theme	tissue-specific	1917:1931	arg1	peaks					1933:1937	tissue-specific peaks	1917:1937	tissue-specific peaks	1917:1937	The best matrices for the untargeted detection of unknown lipids in mouse hind legs in positive ion mode were CHCA and DHB based on the number of tissue-specific peaks and signal-to-noise ratios.
32930817	1	76	theme	past	100:103	arg1	decades					105:111	the past decades	96:111	the past decades	96:111	In the past decades, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been applied to a broad range of biological samples, e.g., forensics and preclinical samples.
32930817	6	77	theme	%	992:992	arg1	CMC					994:996	7.5% CMC	989:996	7.5% CMC	989:996	The composition of 20% gelatin and 7.5% CMC showed to support the tissue best while sectioning.
32930817	11	78	theme	bone	1626:1629	arg1	tissue					1631:1636	bone tissue	1626:1636	bone tissue	1626:1636	The limit of detection (LOD) is estimated to approximately 50 pg/spot on bone tissue.
32930817	14	79	theme	preparation	2002:2012	arg1	method					2014:2019	The developed and optimized sample preparation method	1967:2019	The developed and optimized sample preparation method	1967:2019	The developed and optimized sample preparation method, applicable on animal and human bones, opens the door for future forensic and (pre)clinical investigations.
32930817	1	80	theme	laser	130:134	arg1	spectrometry					163:174	matrix-assisted laser desorption/ionization mass spectrometry	114:174	matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI)	114:194	In the past decades, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been applied to a broad range of biological samples, e.g., forensics and preclinical samples.
32930817	6	81	theme	%	975:975	arg1	gelatin					977:983	20% gelatin	973:983	20% gelatin	973:983	The composition of 20% gelatin and 7.5% CMC showed to support the tissue best while sectioning.
32930817	8	82	theme	sectioning	1259:1268	arg1	applicable					1293:1302	applicable	1293:1302	applicable	1293:1302	The developed sectioning method was shown to be applicable on rat and mouse as well as human bone samples.
32930817	8	82	theme	sectioning	1259:1268	arg1	method					1270:1275	The developed sectioning method	1245:1275	The developed sectioning method	1245:1275	The developed sectioning method was shown to be applicable on rat and mouse as well as human bone samples.
32930817	0	83	theme	clinical	70:77	arg1	applications					79:90	forensic and (pre)clinical applications	52:90	forensic and (pre)clinical applications	52:90	Sample preparation of bone tissue for MALDI-MSI for forensic and (pre)clinical applications.
32930817	5	84	theme	embedding	933:941	arg1	materials					943:951	embedding materials	933:951	embedding materials	933:951	Different concentrations of gelatin and carboxymethyl cellulose (CMC) were tested as embedding materials.
32930817	5	84	theme	embedding	933:941	arg1	concentrations					858:871	Different concentrations	848:871	Different concentrations of gelatin and carboxymethyl cellulose (CMC)	848:916	Different concentrations of gelatin and carboxymethyl cellulose (CMC) were tested as embedding materials.
32930817	2	85	theme	preparation	465:475	arg1	protocol					477:484	a proper sample preparation protocol	449:484	a proper sample preparation protocol for undecalcified bone tissue	449:514	The use of MALDI-MSI for the analysis of bone tissue has been limited due to the insulating properties of the material but more importantly the absence of a proper sample preparation protocol for undecalcified bone tissue.
32930817	7	86	from	knife	1106:1110	arg1	fashion					1130:1136	a longitudinal fashion	1115:1136	a longitudinal fashion	1115:1136	Bone tissue has to be sectioned with a tungsten carbide knife in a longitudinal fashion, while the sections need to be supported with double-sided tapes to maintain the morphology of the tissue.
32930817	4	87	theme	undecalcified	712:724	arg1	samples					731:737	undecalcified bone samples	712:737	undecalcified bone samples	712:737	Here, we optimized the sample preparation protocol of undecalcified bone samples, aimed at both targeted and untargeted applications for forensic and preclinical applications, respectively.
32930817	2	88	theme	proper	451:456	arg1	preparation					465:475	a proper sample preparation	449:475	a proper sample preparation protocol for undecalcified bone tissue	449:514	The use of MALDI-MSI for the analysis of bone tissue has been limited due to the insulating properties of the material but more importantly the absence of a proper sample preparation protocol for undecalcified bone tissue.
32930817	4	89	theme	samples	731:737	arg1	protocol					700:707	the sample preparation protocol	677:707	the sample preparation protocol of undecalcified bone samples, aimed at both targeted and untargeted applications for forensic and preclinical applications	677:831	Here, we optimized the sample preparation protocol of undecalcified bone samples, aimed at both targeted and untargeted applications for forensic and preclinical applications, respectively.
32930817	10	90	theme	positive	1534:1541	arg1	mode					1547:1550	positive ion mode	1534:1550	positive ion mode	1534:1550	DHB proved to be the most suitable matrix for the detection of methadone and EDDP in positive ion mode.
32930817	12	91	theme	human	1755:1759	arg1	clavicle					1761:1768	a dosed human clavicle	1747:1768	a dosed human clavicle	1747:1768	The protocol was successfully applied to detect the presence of methadone and EDDP in a dosed rat femur and a dosed human clavicle.
32930817	4	92	theme	sample	681:686	arg1	preparation					688:698	sample preparation	681:698	the sample preparation protocol of undecalcified bone samples, aimed at both targeted and untargeted applications for forensic and preclinical applications	677:831	Here, we optimized the sample preparation protocol of undecalcified bone samples, aimed at both targeted and untargeted applications for forensic and preclinical applications, respectively.
32930817	14	93	theme	forensic	2086:2093	arg1	investigations					2113:2126	future forensic and (pre)clinical investigations	2079:2126	future forensic and (pre)clinical investigations	2079:2126	The developed and optimized sample preparation method, applicable on animal and human bones, opens the door for future forensic and (pre)clinical investigations.
32930817	10	94	theme	EDDP	1526:1529	arg1	detection					1499:1507	the detection	1495:1507	the detection of methadone and EDDP in positive ion mode	1495:1550	DHB proved to be the most suitable matrix for the detection of methadone and EDDP in positive ion mode.
32930817	0	95	theme	bone	22:25	arg1	tissue					27:32	bone tissue	22:32	bone tissue	22:32	Sample preparation of bone tissue for MALDI-MSI for forensic and (pre)clinical applications.
32930817	12	96	theme	dosed	1727:1731	arg1	femur					1737:1741	a dosed rat femur	1725:1741	a dosed rat femur	1725:1741	The protocol was successfully applied to detect the presence of methadone and EDDP in a dosed rat femur and a dosed human clavicle.
32930817	7	97	theme	tissue	1237:1242	arg1	morphology					1219:1228	the morphology	1215:1228	the morphology of the tissue	1215:1242	Bone tissue has to be sectioned with a tungsten carbide knife in a longitudinal fashion, while the sections need to be supported with double-sided tapes to maintain the morphology of the tissue.
32930817	2	98	theme	insulating	375:384	arg1	properties					386:395	the insulating properties	371:395	the insulating properties of the material	371:411	The use of MALDI-MSI for the analysis of bone tissue has been limited due to the insulating properties of the material but more importantly the absence of a proper sample preparation protocol for undecalcified bone tissue.
32930817	7	99	theme	tungsten	1089:1096	arg1	knife					1106:1110	a tungsten carbide knife	1087:1110	a tungsten carbide knife in a longitudinal fashion	1087:1136	Bone tissue has to be sectioned with a tungsten carbide knife in a longitudinal fashion, while the sections need to be supported with double-sided tapes to maintain the morphology of the tissue.
32930817	1	100	theme	forensics	259:267	arg1	range					224:228	a broad range	216:228	a broad range of biological samples, e.g., forensics and preclinical samples	216:291	In the past decades, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been applied to a broad range of biological samples, e.g., forensics and preclinical samples.
32930817	14	101	theme	optimized	1985:1993	arg1	method					2014:2019	The developed and optimized sample preparation method	1967:2019	The developed and optimized sample preparation method	1967:2019	The developed and optimized sample preparation method, applicable on animal and human bones, opens the door for future forensic and (pre)clinical investigations.
32930817	1	102	theme	preclinical	273:283	arg1	samples					285:291	preclinical samples	273:291	preclinical samples	273:291	In the past decades, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been applied to a broad range of biological samples, e.g., forensics and preclinical samples.
32930817	2	103	theme	material	404:411	arg1	properties					386:395	the insulating properties	371:395	the insulating properties of the material	371:411	The use of MALDI-MSI for the analysis of bone tissue has been limited due to the insulating properties of the material but more importantly the absence of a proper sample preparation protocol for undecalcified bone tissue.
32930817	3	104	theme	bio	629:631	arg1	interface					633:641	the tissue-bone bio interface	613:641	the tissue-bone bio interface in particular	613:655	Undecalcified sections are preferred to retain sample integrity as much as possible or to study the tissue-bone bio interface in particular.
32930817	13	105	theme	ion	1867:1869	arg1	mode					1871:1874	positive ion mode	1858:1874	positive ion mode	1858:1874	The best matrices for the untargeted detection of unknown lipids in mouse hind legs in positive ion mode were CHCA and DHB based on the number of tissue-specific peaks and signal-to-noise ratios.
32930817	8	106	theme	human	1332:1336	arg1	samples					1343:1349	human bone samples	1332:1349	human bone samples	1332:1349	The developed sectioning method was shown to be applicable on rat and mouse as well as human bone samples.
32930817	13	107	theme	hind	1845:1848	arg1	legs					1850:1853	mouse hind legs	1839:1853	mouse hind legs in positive ion mode	1839:1874	The best matrices for the untargeted detection of unknown lipids in mouse hind legs in positive ion mode were CHCA and DHB based on the number of tissue-specific peaks and signal-to-noise ratios.
32930817	1	108	theme	broad	218:222	arg1	range					224:228	a broad range	216:228	a broad range of biological samples, e.g., forensics and preclinical samples	216:291	In the past decades, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been applied to a broad range of biological samples, e.g., forensics and preclinical samples.
33309382	8	0	dep	size	1400:1403	arg1	SP6					1406:1408	SP6	1406:1408	SP6	1406:1408	Three orthogonal contrasts were used to compare treatments: effect of kernel processing (PT6 vs. SP6) and effect of TLOC (particle size; SP6 vs. SP12 and SP12 vs. SP18).
33309382	8	0	dep	size	1400:1403	arg1	SP12					1414:1417	SP12	1414:1417	SP12	1414:1417	Three orthogonal contrasts were used to compare treatments: effect of kernel processing (PT6 vs. SP6) and effect of TLOC (particle size; SP6 vs. SP12 and SP12 vs. SP18).
33309382	7	1	dep	diets	1171:1175	arg1	%					1178:1178	%	1178:1178	% of dry matter	1178:1192	Nutrient composition of the diets (% of dry matter) was 16.5% crude protein, 28.9% neutral detergent fiber, and 25.4% starch.
33309382	7	1	dep	diets	1171:1175	arg1	matter					1187:1192	dry matter	1183:1192	dry matter	1183:1192	Nutrient composition of the diets (% of dry matter) was 16.5% crude protein, 28.9% neutral detergent fiber, and 25.4% starch.
33309382	11	2	theme	lactose	1856:1862	arg1	synthesis					1864:1872	lactose synthesis	1856:1872	lactose synthesis by the mammary gland	1856:1893	The mechanism for increased yield of milk and milk components involved greater kernel fragmentation, starch digestibility, and glucose availability for lactose synthesis by the mammary gland.
33309382	9	3	theme	greater	1540:1546	arg1	efficiency					1553:1562	greater feed efficiency	1540:1562	greater feed efficiency compared with PT6	1540:1580	Cows fed SP6 produced 1.2 kg/d greater milk yield with no changes in dry matter intake, resulting in greater feed efficiency compared with PT6.
33309382	9	4	theme	feed	1548:1551	arg1	efficiency					1553:1562	greater feed efficiency	1540:1562	greater feed efficiency compared with PT6	1540:1580	Cows fed SP6 produced 1.2 kg/d greater milk yield with no changes in dry matter intake, resulting in greater feed efficiency compared with PT6.
33309382	19	5	theme	vitreous	2873:2880	arg1	endosperm					2882:2890	vitreous endosperm	2873:2890	vitreous endosperm	2873:2890	Under the conditions of this study, the use of a self-propelled forage harvester with kernel processing set for a 12-mm TLOC is recommended for WPCS from hybrids with vitreous endosperm.
33309382	6	6	theme	acids	1069:1073	arg1	acids					1069:1073	palm fatty acids	1058:1073	palm fatty acids (FA)	1058:1078	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	6	theme	acids	1069:1073	arg1	FA					1076:1077	FA	1076:1077	FA	1076:1077	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	6	theme	acids	1069:1073	arg1	soap					1050:1053	1.8% calcium soap	1037:1053	1.8% calcium soap of palm fatty acids (FA)	1037:1078	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	6	theme	acids	1069:1073	arg1	meal					988:991	9.5% soybean meal	975:991	9.5% soybean meal	975:991	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	6	theme	acids	1069:1073	arg1	mix					1106:1108	vitamin mix	1098:1108	vitamin mix	1098:1108	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	6	theme	acids	1069:1073	arg1	meal					1031:1034	6.8% low rumen degradability soybean meal	994:1034	6.8% low rumen degradability soybean meal	994:1034	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	6	theme	acids	1069:1073	arg1	%					1084:1084	1.7% mineral	1081:1092	1.7% mineral	1081:1092	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	7	7	dep	%	1224:1224	arg1	fiber					1244:1248	neutral detergent fiber	1226:1248	28.9% neutral detergent fiber	1220:1248	Nutrient composition of the diets (% of dry matter) was 16.5% crude protein, 28.9% neutral detergent fiber, and 25.4% starch.
33309382	14	8	theme	lower	2149:2153	arg1	digestibility					2174:2186	lower total-tract starch digestibility	2149:2186	lower total-tract starch digestibility	2149:2186	Cows fed SP18 had lower total-tract starch digestibility and tended to have lower plasma glucose and produce less milk compared with cows fed SP12.
33309382	6	9	theme	citrus	939:944	arg1	pulp					946:949	15.5% citrus pulp	933:949	15.5% citrus pulp	933:949	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	15	10	theme	feeding	2298:2304	arg1	SP6					2306:2308	feeding SP6	2298:2308	feeding SP6	2298:2308	Compared with PT6, feeding SP6 raised linear odd-chain FA concentration in milk.
33309382	6	11	theme	%	1116:1116	arg1	urea					1118:1121	1% urea	1115:1121	1% urea (dry matter basis)	1115:1140	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	11	theme	%	1116:1116	arg1	basis					1135:1139	dry matter basis	1124:1139	dry matter basis	1124:1139	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	5	12	dep	±	779:779	arg1	d					784:784	63 d	781:784	139 ± 63 d in milk	775:792	Twenty-four Holstein cows (139 ± 63 d in milk) were blocked and assigned to six 4 × 4 Latin squares with 24-d periods (18 d of adaptation).
33309382	18	13	from	indication	2647:2656	arg1	rumen					2699:2703	the rumen	2695:2703	the rumen	2695:2703	Lesser trans C18:1 followed by greater C18:0 concentrations were observed for SP12 and PT6 compared with SP6, which is an indication of more complete biohydrogenation in the rumen.
33309382	6	14	theme	15.5	933:936	arg1	%					937:937	%	937:937	%	937:937	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	1	15	theme	cut	167:169	arg1	processing					130:139	Kernel processing	123:139	Kernel processing	123:139	Kernel processing and theoretical length of cut (TLOC) of whole-plant corn silage (WPCS) can affect feed intake, digestibility, and performance of dairy cows.
33309382	1	15	theme	cut	167:169	arg1	length					157:162	theoretical length	145:162	theoretical length	145:162	Kernel processing and theoretical length of cut (TLOC) of whole-plant corn silage (WPCS) can affect feed intake, digestibility, and performance of dairy cows.
33309382	13	16	theme	serum	2088:2092	arg1	A					2102:2102	less serum amyloid A	2083:2102	less serum amyloid A	2083:2102	Milk yield was similar for SP6 and SP12, but SP12 cows tended to have less serum amyloid A with greater chewing time.
33309382	17	17	theme	milk	2476:2479	arg1	proportion					2462:2471	greater proportion	2454:2471	greater proportion of milk C14:1 and C16:1	2454:2495	Cows fed SP6 had greater proportion of milk C14:1 and C16:1 compared with PT6 and SP12.
33309382	14	18	theme	plasma	2213:2218	arg1	glucose					2220:2226	lower plasma glucose	2207:2226	lower plasma glucose	2207:2226	Cows fed SP18 had lower total-tract starch digestibility and tended to have lower plasma glucose and produce less milk compared with cows fed SP12.
33309382	3	19	theme	6-mm	510:513	arg1	PT6					521:523	PT6	521:523	PT6	521:523	The treatments were a pull-type forage harvester without kernel processor set for a 6-mm TLOC (PT6) and a self-propelled forage harvester with kernel processor set for a 6-mm TLOC (SP6), 12-mm TLOC (SP12), and 18-mm TLOC (SP18).
33309382	3	19	theme	6-mm	510:513	arg1	TLOC					515:518	a 6-mm TLOC	508:518	a 6-mm TLOC (PT6)	508:524	The treatments were a pull-type forage harvester without kernel processor set for a 6-mm TLOC (PT6) and a self-propelled forage harvester with kernel processor set for a 6-mm TLOC (SP6), 12-mm TLOC (SP12), and 18-mm TLOC (SP18).
33309382	3	20	with	harvester	554:562	arg1	processor					576:584	kernel processor	569:584	kernel processor set for a 6-mm TLOC (SP6), 12-mm TLOC (SP12), and 18-mm TLOC (SP18)	569:652	The treatments were a pull-type forage harvester without kernel processor set for a 6-mm TLOC (PT6) and a self-propelled forage harvester with kernel processor set for a 6-mm TLOC (SP6), 12-mm TLOC (SP12), and 18-mm TLOC (SP18).
33309382	7	21	theme	dry	1183:1185	arg1	matter					1187:1192	dry matter	1183:1192	dry matter	1183:1192	Nutrient composition of the diets (% of dry matter) was 16.5% crude protein, 28.9% neutral detergent fiber, and 25.4% starch.
33309382	18	22	theme	trans	2532:2536	arg1	C18:1					2538:2542	Lesser trans C18:1	2525:2542	Lesser trans C18:1 followed by greater C18:0 concentrations	2525:2583	Lesser trans C18:1 followed by greater C18:0 concentrations were observed for SP12 and PT6 compared with SP6, which is an indication of more complete biohydrogenation in the rumen.
33309382	0	23	theme	cow	106:108	arg1	performance					110:120	dairy cow performance	100:120	dairy cow performance	100:120	Effect of kernel processing and particle size of whole-plant corn silage with vitreous endosperm on dairy cow performance.
33309382	19	24	theme	study	2735:2739	arg1	conditions					2716:2725	the conditions	2712:2725	the conditions of this study	2712:2739	Under the conditions of this study, the use of a self-propelled forage harvester with kernel processing set for a 12-mm TLOC is recommended for WPCS from hybrids with vitreous endosperm.
33309382	19	25	dep	harvester	2777:2785	arg1	forage					2770:2775	forage	2770:2775	forage	2770:2775	Under the conditions of this study, the use of a self-propelled forage harvester with kernel processing set for a 12-mm TLOC is recommended for WPCS from hybrids with vitreous endosperm.
33309382	6	26	theme	%	925:925	arg1	WPCS					927:930	48.5% WPCS	921:930	48.5% WPCS	921:930	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	8	27	theme	particle	1391:1398	arg1	size					1400:1403	particle size	1391:1403	particle size	1391:1403	Three orthogonal contrasts were used to compare treatments: effect of kernel processing (PT6 vs. SP6) and effect of TLOC (particle size; SP6 vs. SP12 and SP12 vs. SP18).
33309382	19	28	theme	self-propelled	2755:2768	arg1	harvester					2777:2785	a self-propelled forage harvester	2753:2785	a self-propelled forage harvester with kernel processing set for a 12-mm TLOC	2753:2829	Under the conditions of this study, the use of a self-propelled forage harvester with kernel processing set for a 12-mm TLOC is recommended for WPCS from hybrids with vitreous endosperm.
33309382	1	29	theme	corn	193:196	arg1	WPCS					206:209	WPCS	206:209	WPCS	206:209	Kernel processing and theoretical length of cut (TLOC) of whole-plant corn silage (WPCS) can affect feed intake, digestibility, and performance of dairy cows.
33309382	1	29	theme	corn	193:196	arg1	silage					198:203	whole-plant corn silage	181:203	whole-plant corn silage (WPCS)	181:210	Kernel processing and theoretical length of cut (TLOC) of whole-plant corn silage (WPCS) can affect feed intake, digestibility, and performance of dairy cows.
33309382	7	30	theme	diets	1171:1175	arg1	composition					1152:1162	Nutrient composition	1143:1162	Nutrient composition of the diets (% of dry matter)	1143:1193	Nutrient composition of the diets (% of dry matter) was 16.5% crude protein, 28.9% neutral detergent fiber, and 25.4% starch.
33309382	18	31	theme	greater	2556:2562	arg1	concentrations					2570:2583	greater C18:0 concentrations	2556:2583	greater C18:0 concentrations	2556:2583	Lesser trans C18:1 followed by greater C18:0 concentrations were observed for SP12 and PT6 compared with SP6, which is an indication of more complete biohydrogenation in the rumen.
33309382	11	32	theme	mammary	1881:1887	arg1	gland					1889:1893	the mammary gland	1877:1893	the mammary gland	1877:1893	The mechanism for increased yield of milk and milk components involved greater kernel fragmentation, starch digestibility, and glucose availability for lactose synthesis by the mammary gland.
33309382	11	33	theme	kernel	1783:1788	arg1	fragmentation					1790:1802	greater kernel fragmentation	1775:1802	greater kernel fragmentation	1775:1802	The mechanism for increased yield of milk and milk components involved greater kernel fragmentation, starch digestibility, and glucose availability for lactose synthesis by the mammary gland.
33309382	3	34	theme	18-mm	636:640	arg1	TLOC					642:645	18-mm TLOC	636:645	18-mm TLOC (SP18)	636:652	The treatments were a pull-type forage harvester without kernel processor set for a 6-mm TLOC (PT6) and a self-propelled forage harvester with kernel processor set for a 6-mm TLOC (SP6), 12-mm TLOC (SP12), and 18-mm TLOC (SP18).
33309382	3	34	theme	18-mm	636:640	arg1	SP18					648:651	SP18	648:651	SP18	648:651	The treatments were a pull-type forage harvester without kernel processor set for a 6-mm TLOC (PT6) and a self-propelled forage harvester with kernel processor set for a 6-mm TLOC (SP6), 12-mm TLOC (SP12), and 18-mm TLOC (SP18).
33309382	6	35	theme	ground	962:967	arg1	corn					969:972	15.0% dry ground corn	952:972	15.0% dry ground corn	952:972	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	12	36	theme	greater	1960:1966	arg1	levels					1968:1973	greater levels	1960:1973	greater levels of serum amyloid A	1960:1992	However, cows fed SP6 had lower chewing time and tended to have greater levels of serum amyloid A compared with PT6.
33309382	12	37	theme	lower	1922:1926	arg1	time					1936:1939	lower chewing time	1922:1939	lower chewing time	1922:1939	However, cows fed SP6 had lower chewing time and tended to have greater levels of serum amyloid A compared with PT6.
33309382	8	38	theme	TLOC	1385:1388	arg1	effect					1375:1380	effect	1375:1380	effect of TLOC (particle size; SP6 vs. SP12 and SP12 vs. SP18)	1375:1436	Three orthogonal contrasts were used to compare treatments: effect of kernel processing (PT6 vs. SP6) and effect of TLOC (particle size; SP6 vs. SP12 and SP12 vs. SP18).
33309382	8	38	theme	TLOC	1385:1388	arg1	effect					1329:1334	effect	1329:1334	effect of kernel processing (PT6 vs. SP6)	1329:1369	Three orthogonal contrasts were used to compare treatments: effect of kernel processing (PT6 vs. SP6) and effect of TLOC (particle size; SP6 vs. SP12 and SP12 vs. SP18).
33309382	12	39	theme	A	1992:1992	arg1	levels					1968:1973	greater levels	1960:1973	greater levels of serum amyloid A	1960:1992	However, cows fed SP6 had lower chewing time and tended to have greater levels of serum amyloid A compared with PT6.
33309382	6	40	theme	%	956:956	arg1	corn					969:972	15.0% dry ground corn	952:972	15.0% dry ground corn	952:972	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	19	41	theme	kernel	2792:2797	arg1	set					2810:2812	kernel processing set	2792:2812	kernel processing set	2792:2812	Under the conditions of this study, the use of a self-propelled forage harvester with kernel processing set for a 12-mm TLOC is recommended for WPCS from hybrids with vitreous endosperm.
33309382	6	42	theme	matter	1128:1133	arg1	urea					1118:1121	1% urea	1115:1121	1% urea (dry matter basis)	1115:1140	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	42	theme	matter	1128:1133	arg1	basis					1135:1139	dry matter basis	1124:1139	dry matter basis	1124:1139	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	1	43	theme	Kernel	123:128	arg1	processing					130:139	Kernel processing	123:139	Kernel processing	123:139	Kernel processing and theoretical length of cut (TLOC) of whole-plant corn silage (WPCS) can affect feed intake, digestibility, and performance of dairy cows.
33309382	7	44	theme	Nutrient	1143:1150	arg1	composition					1152:1162	Nutrient composition	1143:1162	Nutrient composition of the diets (% of dry matter)	1143:1193	Nutrient composition of the diets (% of dry matter) was 16.5% crude protein, 28.9% neutral detergent fiber, and 25.4% starch.
33309382	18	45	theme	biohydrogenation	2675:2690	arg1	SP6					2630:2632	SP6	2630:2632	SP6	2630:2632	Lesser trans C18:1 followed by greater C18:0 concentrations were observed for SP12 and PT6 compared with SP6, which is an indication of more complete biohydrogenation in the rumen.
33309382	18	45	theme	biohydrogenation	2675:2690	arg1	indication					2647:2656	an indication	2644:2656	an indication of more complete biohydrogenation in the rumen	2644:2703	Lesser trans C18:1 followed by greater C18:0 concentrations were observed for SP12 and PT6 compared with SP6, which is an indication of more complete biohydrogenation in the rumen.
33309382	10	46	theme	milk	1615:1618	arg1	g/d					1633:1635	+36 g/d	1629:1635	+36 g/d	1629:1635	Cows fed SP6 also produced more milk protein (+36 g/d), lactose (+61 g/d), and total solids (+94 g/d) than cows fed PT6.
33309382	10	46	theme	milk	1615:1618	arg1	protein					1620:1626	more milk protein	1610:1626	more milk protein (+36 g/d)	1610:1636	Cows fed SP6 also produced more milk protein (+36 g/d), lactose (+61 g/d), and total solids (+94 g/d) than cows fed PT6.
33309382	19	47	with	harvester	2777:2785	arg1	set					2810:2812	kernel processing set	2792:2812	kernel processing set	2792:2812	Under the conditions of this study, the use of a self-propelled forage harvester with kernel processing set for a 12-mm TLOC is recommended for WPCS from hybrids with vitreous endosperm.
33309382	6	48	theme	degradability	1009:1021	arg1	meal					1031:1034	6.8% low rumen degradability soybean meal	994:1034	6.8% low rumen degradability soybean meal	994:1034	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	48	theme	degradability	1009:1021	arg1	meal					988:991	9.5% soybean meal	975:991	9.5% soybean meal	975:991	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	0	49	theme	kernel	10:15	arg1	processing					17:26	kernel processing	10:26	kernel processing	10:26	Effect of kernel processing and particle size of whole-plant corn silage with vitreous endosperm on dairy cow performance.
33309382	7	50	theme	neutral	1226:1232	arg1	fiber					1244:1248	neutral detergent fiber	1226:1248	28.9% neutral detergent fiber	1220:1248	Nutrient composition of the diets (% of dry matter) was 16.5% crude protein, 28.9% neutral detergent fiber, and 25.4% starch.
33309382	14	51	theme	total-tract	2155:2165	arg1	digestibility					2174:2186	lower total-tract starch digestibility	2149:2186	lower total-tract starch digestibility	2149:2186	Cows fed SP18 had lower total-tract starch digestibility and tended to have lower plasma glucose and produce less milk compared with cows fed SP12.
33309382	8	52	used	used	1301:1304	arg2	orthogonal					1275:1284	orthogonal	1275:1284	orthogonal	1275:1284	Three orthogonal contrasts were used to compare treatments: effect of kernel processing (PT6 vs. SP6) and effect of TLOC (particle size; SP6 vs. SP12 and SP12 vs. SP18).
33309382	12	53	theme	serum	1978:1982	arg1	A					1992:1992	serum amyloid A	1978:1992	serum amyloid A	1978:1992	However, cows fed SP6 had lower chewing time and tended to have greater levels of serum amyloid A compared with PT6.
33309382	0	54	theme	silage	66:71	arg1	processing					17:26	kernel processing	10:26	kernel processing	10:26	Effect of kernel processing and particle size of whole-plant corn silage with vitreous endosperm on dairy cow performance.
33309382	0	54	theme	silage	66:71	arg1	size					41:44	particle size	32:44	particle size	32:44	Effect of kernel processing and particle size of whole-plant corn silage with vitreous endosperm on dairy cow performance.
33309382	13	55	theme	amyloid	2094:2100	arg1	A					2102:2102	less serum amyloid A	2083:2102	less serum amyloid A	2083:2102	Milk yield was similar for SP6 and SP12, but SP12 cows tended to have less serum amyloid A with greater chewing time.
33309382	17	56	dep	proportion	2462:2471	arg1	C16:1					2491:2495	C16:1	2491:2495	C16:1	2491:2495	Cows fed SP6 had greater proportion of milk C14:1 and C16:1 compared with PT6 and SP12.
33309382	17	56	dep	proportion	2462:2471	arg1	C14:1					2481:2485	C14:1	2481:2485	C14:1	2481:2485	Cows fed SP6 had greater proportion of milk C14:1 and C16:1 compared with PT6 and SP12.
33309382	0	57	from	Effect	0:5	arg1	performance					110:120	dairy cow performance	100:120	dairy cow performance	100:120	Effect of kernel processing and particle size of whole-plant corn silage with vitreous endosperm on dairy cow performance.
33309382	0	58	theme	vitreous	78:85	arg1	endosperm					87:95	vitreous endosperm	78:95	vitreous endosperm	78:95	Effect of kernel processing and particle size of whole-plant corn silage with vitreous endosperm on dairy cow performance.
33309382	13	59	contain	have	2078:2081	arg2	A					2102:2102	less serum amyloid A	2083:2102	less serum amyloid A	2083:2102	Milk yield was similar for SP6 and SP12, but SP12 cows tended to have less serum amyloid A with greater chewing time.
33309382	13	59	contain	have	2078:2081	arg1	cows					2063:2066	SP12 cows	2058:2066	SP12 cows	2058:2066	Milk yield was similar for SP6 and SP12, but SP12 cows tended to have less serum amyloid A with greater chewing time.
33309382	2	60	theme	dairy	340:344	arg1	cows					346:349	lactating dairy cows	330:349	lactating dairy cows	330:349	The objective of this study was to evaluate for lactating dairy cows the effects of kernel processing and TLOC of WPCS with vitreous endosperm.
33309382	9	61	from	changes	1497:1503	arg1	intake					1519:1524	dry matter intake	1508:1524	dry matter intake	1508:1524	Cows fed SP6 produced 1.2 kg/d greater milk yield with no changes in dry matter intake, resulting in greater feed efficiency compared with PT6.
33309382	0	62	theme	particle	32:39	arg1	size					41:44	particle size	32:44	particle size	32:44	Effect of kernel processing and particle size of whole-plant corn silage with vitreous endosperm on dairy cow performance.
33309382	12	63	dep	fed	1910:1912	arg1	tended					1945:1950	tended	1945:1950	tended to have greater levels of serum amyloid A compared with PT6	1945:2010	However, cows fed SP6 had lower chewing time and tended to have greater levels of serum amyloid A compared with PT6.
33309382	12	63	dep	fed	1910:1912	arg1	had					1918:1920	had	1918:1920	had lower chewing time	1918:1939	However, cows fed SP6 had lower chewing time and tended to have greater levels of serum amyloid A compared with PT6.
33309382	2	64	theme	TLOC	388:391	arg1	effects					355:361	the effects	351:361	the effects of kernel processing and TLOC of WPCS with vitreous endosperm	351:423	The objective of this study was to evaluate for lactating dairy cows the effects of kernel processing and TLOC of WPCS with vitreous endosperm.
33309382	5	65	dep	periods	858:864	arg1	d					870:870	18 d	867:870	18 d of adaptation	867:884	Twenty-four Holstein cows (139 ± 63 d in milk) were blocked and assigned to six 4 × 4 Latin squares with 24-d periods (18 d of adaptation).
33309382	0	66	theme	corn	61:64	arg1	silage					66:71	whole-plant corn silage	49:71	whole-plant corn silage	49:71	Effect of kernel processing and particle size of whole-plant corn silage with vitreous endosperm on dairy cow performance.
33309382	19	67	theme	processing	2799:2808	arg1	set					2810:2812	kernel processing set	2792:2812	kernel processing set	2792:2812	Under the conditions of this study, the use of a self-propelled forage harvester with kernel processing set for a 12-mm TLOC is recommended for WPCS from hybrids with vitreous endosperm.
33309382	8	68	theme	processing	1346:1355	arg1	effect					1375:1380	effect	1375:1380	effect of TLOC (particle size; SP6 vs. SP12 and SP12 vs. SP18)	1375:1436	Three orthogonal contrasts were used to compare treatments: effect of kernel processing (PT6 vs. SP6) and effect of TLOC (particle size; SP6 vs. SP12 and SP12 vs. SP18).
33309382	8	68	theme	processing	1346:1355	arg1	effect					1329:1334	effect	1329:1334	effect of kernel processing (PT6 vs. SP6)	1329:1369	Three orthogonal contrasts were used to compare treatments: effect of kernel processing (PT6 vs. SP6) and effect of TLOC (particle size; SP6 vs. SP12 and SP12 vs. SP18).
33309382	2	69	theme	processing	373:382	arg1	effects					355:361	the effects	351:361	the effects of kernel processing and TLOC of WPCS with vitreous endosperm	351:423	The objective of this study was to evaluate for lactating dairy cows the effects of kernel processing and TLOC of WPCS with vitreous endosperm.
33309382	6	70	contain	contain	913:919	arg2	pulp					946:949	15.5% citrus pulp	933:949	15.5% citrus pulp	933:949	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	70	contain	contain	913:919	arg2	corn					969:972	15.0% dry ground corn	952:972	15.0% dry ground corn	952:972	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	70	contain	contain	913:919	arg2	%					1084:1084	1.7% mineral	1081:1092	1.7% mineral	1081:1092	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	70	contain	contain	913:919	arg2	urea					1118:1121	1% urea	1115:1121	1% urea (dry matter basis)	1115:1140	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	70	contain	contain	913:919	arg2	mix					1106:1108	vitamin mix	1098:1108	vitamin mix	1098:1108	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	70	contain	contain	913:919	arg2	meal					1031:1034	6.8% low rumen degradability soybean meal	994:1034	6.8% low rumen degradability soybean meal	994:1034	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	70	contain	contain	913:919	arg2	basis					1135:1139	dry matter basis	1124:1139	dry matter basis	1124:1139	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	70	contain	contain	913:919	arg2	acids					1069:1073	palm fatty acids	1058:1073	palm fatty acids (FA)	1058:1078	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	70	contain	contain	913:919	arg2	meal					988:991	9.5% soybean meal	975:991	9.5% soybean meal	975:991	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	70	contain	contain	913:919	arg2	WPCS					927:930	48.5% WPCS	921:930	48.5% WPCS	921:930	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	70	contain	contain	913:919	arg2	soap					1050:1053	1.8% calcium soap	1037:1053	1.8% calcium soap of palm fatty acids (FA)	1037:1078	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	70	contain	contain	913:919	arg1	Diets					888:892	Diets	888:892	Diets	888:892	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	11	71	theme	milk	1741:1744	arg1	components					1755:1764	milk and milk components	1741:1764	components	1755:1764	The mechanism for increased yield of milk and milk components involved greater kernel fragmentation, starch digestibility, and glucose availability for lactose synthesis by the mammary gland.
33309382	6	72	theme	low	999:1001	arg1	meal					1031:1034	6.8% low rumen degradability soybean meal	994:1034	6.8% low rumen degradability soybean meal	994:1034	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	72	theme	low	999:1001	arg1	meal					988:991	9.5% soybean meal	975:991	9.5% soybean meal	975:991	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	73	theme	calcium	1042:1048	arg1	soap					1050:1053	1.8% calcium soap	1037:1053	1.8% calcium soap of palm fatty acids (FA)	1037:1078	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	73	theme	calcium	1042:1048	arg1	meal					988:991	9.5% soybean meal	975:991	9.5% soybean meal	975:991	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	5	74	theme	adaptation	875:884	arg1	d					870:870	18 d	867:870	18 d of adaptation	867:884	Twenty-four Holstein cows (139 ± 63 d in milk) were blocked and assigned to six 4 × 4 Latin squares with 24-d periods (18 d of adaptation).
33309382	7	75	theme	matter	1187:1192	arg1	%					1178:1178	%	1178:1178	% of dry matter	1178:1192	Nutrient composition of the diets (% of dry matter) was 16.5% crude protein, 28.9% neutral detergent fiber, and 25.4% starch.
33309382	7	75	theme	matter	1187:1192	arg1	matter					1187:1192	dry matter	1183:1192	dry matter	1183:1192	Nutrient composition of the diets (% of dry matter) was 16.5% crude protein, 28.9% neutral detergent fiber, and 25.4% starch.
33309382	2	76	theme	vitreous	406:413	arg1	endosperm					415:423	vitreous endosperm	406:423	vitreous endosperm	406:423	The objective of this study was to evaluate for lactating dairy cows the effects of kernel processing and TLOC of WPCS with vitreous endosperm.
33309382	19	77	theme	12-mm	2820:2824	arg1	TLOC					2826:2829	a 12-mm TLOC	2818:2829	a 12-mm TLOC	2818:2829	Under the conditions of this study, the use of a self-propelled forage harvester with kernel processing set for a 12-mm TLOC is recommended for WPCS from hybrids with vitreous endosperm.
33309382	6	78	theme	1.8	1037:1039	arg1	%					1040:1040	%	1040:1040	%	1040:1040	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	5	79	theme	24-d	853:856	arg1	periods					858:864	24-d periods	853:864	24-d periods (18 d of adaptation)	853:885	Twenty-four Holstein cows (139 ± 63 d in milk) were blocked and assigned to six 4 × 4 Latin squares with 24-d periods (18 d of adaptation).
33309382	2	80	theme	WPCS	396:399	arg1	TLOC					388:391	TLOC	388:391	TLOC	388:391	The objective of this study was to evaluate for lactating dairy cows the effects of kernel processing and TLOC of WPCS with vitreous endosperm.
33309382	2	80	theme	WPCS	396:399	arg1	processing					373:382	kernel processing	366:382	kernel processing	366:382	The objective of this study was to evaluate for lactating dairy cows the effects of kernel processing and TLOC of WPCS with vitreous endosperm.
33309382	0	81	theme	whole-plant	49:59	arg1	silage					66:71	whole-plant corn silage	49:71	whole-plant corn silage	49:71	Effect of kernel processing and particle size of whole-plant corn silage with vitreous endosperm on dairy cow performance.
33309382	0	82	theme	size	41:44	arg1	Effect					0:5	Effect	0:5	Effect of kernel processing and particle size of whole-plant corn silage with vitreous endosperm on dairy cow performance.	0:121	Effect of kernel processing and particle size of whole-plant corn silage with vitreous endosperm on dairy cow performance.
33309382	1	83	theme	dairy	270:274	arg1	cows					276:279	dairy cows	270:279	dairy cows	270:279	Kernel processing and theoretical length of cut (TLOC) of whole-plant corn silage (WPCS) can affect feed intake, digestibility, and performance of dairy cows.
33309382	14	84	contain	have	2202:2205	arg1	Cows					2131:2134	Cows	2131:2134	Cows fed SP18	2131:2143	Cows fed SP18 had lower total-tract starch digestibility and tended to have lower plasma glucose and produce less milk compared with cows fed SP12.
33309382	14	84	contain	have	2202:2205	arg2	glucose					2220:2226	lower plasma glucose	2207:2226	lower plasma glucose	2207:2226	Cows fed SP18 had lower total-tract starch digestibility and tended to have lower plasma glucose and produce less milk compared with cows fed SP12.
33309382	8	85	dep	used	1301:1304	arg1	effect					1375:1380	effect	1375:1380	effect of TLOC (particle size; SP6 vs. SP12 and SP12 vs. SP18)	1375:1436	Three orthogonal contrasts were used to compare treatments: effect of kernel processing (PT6 vs. SP6) and effect of TLOC (particle size; SP6 vs. SP12 and SP12 vs. SP18).
33309382	8	85	dep	used	1301:1304	arg1	effect					1329:1334	effect	1329:1334	effect of kernel processing (PT6 vs. SP6)	1329:1369	Three orthogonal contrasts were used to compare treatments: effect of kernel processing (PT6 vs. SP6) and effect of TLOC (particle size; SP6 vs. SP12 and SP12 vs. SP18).
33309382	17	86	contain	had	2450:2452	arg2	proportion					2462:2471	greater proportion	2454:2471	greater proportion of milk C14:1 and C16:1	2454:2495	Cows fed SP6 had greater proportion of milk C14:1 and C16:1 compared with PT6 and SP12.
33309382	17	86	contain	had	2450:2452	arg1	Cows					2437:2440	Cows	2437:2440	Cows fed SP6	2437:2448	Cows fed SP6 had greater proportion of milk C14:1 and C16:1 compared with PT6 and SP12.
33309382	3	87	theme	6-mm	596:599	arg1	SP6					607:609	SP6	607:609	SP6	607:609	The treatments were a pull-type forage harvester without kernel processor set for a 6-mm TLOC (PT6) and a self-propelled forage harvester with kernel processor set for a 6-mm TLOC (SP6), 12-mm TLOC (SP12), and 18-mm TLOC (SP18).
33309382	3	87	theme	6-mm	596:599	arg1	TLOC					601:604	a 6-mm TLOC	594:604	a 6-mm TLOC (SP6)	594:610	The treatments were a pull-type forage harvester without kernel processor set for a 6-mm TLOC (PT6) and a self-propelled forage harvester with kernel processor set for a 6-mm TLOC (SP6), 12-mm TLOC (SP12), and 18-mm TLOC (SP18).
33309382	13	88	theme	SP12	2058:2061	arg1	cows					2063:2066	SP12 cows	2058:2066	SP12 cows	2058:2066	Milk yield was similar for SP6 and SP12, but SP12 cows tended to have less serum amyloid A with greater chewing time.
33309382	1	89	theme	silage	198:203	arg1	TLOC					172:175	TLOC	172:175	TLOC	172:175	Kernel processing and theoretical length of cut (TLOC) of whole-plant corn silage (WPCS) can affect feed intake, digestibility, and performance of dairy cows.
33309382	1	89	theme	silage	198:203	arg1	cut					167:169	cut	167:169	cut (TLOC) of whole-plant corn silage (WPCS)	167:210	Kernel processing and theoretical length of cut (TLOC) of whole-plant corn silage (WPCS) can affect feed intake, digestibility, and performance of dairy cows.
33309382	2	90	with	WPCS	396:399	arg1	endosperm					415:423	vitreous endosperm	406:423	vitreous endosperm	406:423	The objective of this study was to evaluate for lactating dairy cows the effects of kernel processing and TLOC of WPCS with vitreous endosperm.
33309382	15	91	theme	FA	2334:2335	arg1	concentration					2337:2349	linear odd-chain FA concentration	2317:2349	linear odd-chain FA concentration	2317:2349	Compared with PT6, feeding SP6 raised linear odd-chain FA concentration in milk.
33309382	0	92	with	size	41:44	arg1	endosperm					87:95	vitreous endosperm	78:95	vitreous endosperm	78:95	Effect of kernel processing and particle size of whole-plant corn silage with vitreous endosperm on dairy cow performance.
33309382	3	93	theme	pull-type	448:456	arg1	harvester					465:473	a pull-type forage harvester	446:473	a pull-type forage harvester	446:473	The treatments were a pull-type forage harvester without kernel processor set for a 6-mm TLOC (PT6) and a self-propelled forage harvester with kernel processor set for a 6-mm TLOC (SP6), 12-mm TLOC (SP12), and 18-mm TLOC (SP18).
33309382	3	93	theme	pull-type	448:456	arg1	treatments					430:439	The treatments	426:439	The treatments	426:439	The treatments were a pull-type forage harvester without kernel processor set for a 6-mm TLOC (PT6) and a self-propelled forage harvester with kernel processor set for a 6-mm TLOC (SP6), 12-mm TLOC (SP12), and 18-mm TLOC (SP18).
33309382	19	94	theme	harvester	2777:2785	arg1	use					2746:2748	the use	2742:2748	the use of a self-propelled forage harvester with kernel processing set for a 12-mm TLOC	2742:2829	Under the conditions of this study, the use of a self-propelled forage harvester with kernel processing set for a 12-mm TLOC is recommended for WPCS from hybrids with vitreous endosperm.
33309382	15	95	theme	linear	2317:2322	arg1	concentration					2337:2349	linear odd-chain FA concentration	2317:2349	linear odd-chain FA concentration	2317:2349	Compared with PT6, feeding SP6 raised linear odd-chain FA concentration in milk.
33309382	7	96	dep	%	1259:1259	arg1	starch					1261:1266	starch	1261:1266	25.4% starch	1255:1266	Nutrient composition of the diets (% of dry matter) was 16.5% crude protein, 28.9% neutral detergent fiber, and 25.4% starch.
33309382	6	97	theme	rumen	1003:1007	arg1	meal					1031:1034	6.8% low rumen degradability soybean meal	994:1034	6.8% low rumen degradability soybean meal	994:1034	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	97	theme	rumen	1003:1007	arg1	meal					988:991	9.5% soybean meal	975:991	9.5% soybean meal	975:991	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	98	theme	15.0	952:955	arg1	%					956:956	%	956:956	%	956:956	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	2	99	theme	study	304:308	arg1	objective					286:294	The objective	282:294	The objective of this study	282:308	The objective of this study was to evaluate for lactating dairy cows the effects of kernel processing and TLOC of WPCS with vitreous endosperm.
33309382	9	100	theme	dry	1508:1510	arg1	intake					1519:1524	dry matter intake	1508:1524	dry matter intake	1508:1524	Cows fed SP6 produced 1.2 kg/d greater milk yield with no changes in dry matter intake, resulting in greater feed efficiency compared with PT6.
33309382	3	101	theme	kernel	483:488	arg1	processor					490:498	kernel processor	483:498	kernel processor set for a 6-mm TLOC (PT6) and a self-propelled forage harvester with kernel processor set for a 6-mm TLOC (SP6), 12-mm TLOC (SP12), and 18-mm TLOC (SP18)	483:652	The treatments were a pull-type forage harvester without kernel processor set for a 6-mm TLOC (PT6) and a self-propelled forage harvester with kernel processor set for a 6-mm TLOC (SP6), 12-mm TLOC (SP12), and 18-mm TLOC (SP18).
33309382	6	102	theme	fatty	1063:1067	arg1	FA					1076:1077	FA	1076:1077	FA	1076:1077	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	102	theme	fatty	1063:1067	arg1	acids					1069:1073	palm fatty acids	1058:1073	palm fatty acids (FA)	1058:1078	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	1	103	theme	feed	223:226	arg1	intake					228:233	feed intake	223:233	feed intake	223:233	Kernel processing and theoretical length of cut (TLOC) of whole-plant corn silage (WPCS) can affect feed intake, digestibility, and performance of dairy cows.
33309382	18	104	theme	C18:0	2564:2568	arg1	concentrations					2570:2583	greater C18:0 concentrations	2556:2583	greater C18:0 concentrations	2556:2583	Lesser trans C18:1 followed by greater C18:0 concentrations were observed for SP12 and PT6 compared with SP6, which is an indication of more complete biohydrogenation in the rumen.
33309382	8	105	dep	processing	1346:1355	arg1	SP6					1366:1368	SP6	1366:1368	SP6	1366:1368	Three orthogonal contrasts were used to compare treatments: effect of kernel processing (PT6 vs. SP6) and effect of TLOC (particle size; SP6 vs. SP12 and SP12 vs. SP18).
33309382	8	105	dep	processing	1346:1355	arg1	PT6					1358:1360	PT6	1358:1360	PT6	1358:1360	Three orthogonal contrasts were used to compare treatments: effect of kernel processing (PT6 vs. SP6) and effect of TLOC (particle size; SP6 vs. SP12 and SP12 vs. SP18).
33309382	6	106	theme	%	937:937	arg1	pulp					946:949	15.5% citrus pulp	933:949	15.5% citrus pulp	933:949	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	107	theme	1	1115:1115	arg1	%					1116:1116	%	1116:1116	%	1116:1116	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	3	108	theme	12-mm	613:617	arg1	SP12					625:628	SP12	625:628	SP12	625:628	The treatments were a pull-type forage harvester without kernel processor set for a 6-mm TLOC (PT6) and a self-propelled forage harvester with kernel processor set for a 6-mm TLOC (SP6), 12-mm TLOC (SP12), and 18-mm TLOC (SP18).
33309382	3	108	theme	12-mm	613:617	arg1	TLOC					619:622	12-mm TLOC	613:622	12-mm TLOC (SP12)	613:629	The treatments were a pull-type forage harvester without kernel processor set for a 6-mm TLOC (PT6) and a self-propelled forage harvester with kernel processor set for a 6-mm TLOC (SP6), 12-mm TLOC (SP12), and 18-mm TLOC (SP18).
33309382	10	109	theme	total	1662:1666	arg1	solids					1668:1673	total solids	1662:1673	total solids (+94 g/d)	1662:1683	Cows fed SP6 also produced more milk protein (+36 g/d), lactose (+61 g/d), and total solids (+94 g/d) than cows fed PT6.
33309382	10	109	theme	total	1662:1666	arg1	g/d					1680:1682	+94 g/d	1676:1682	+94 g/d	1676:1682	Cows fed SP6 also produced more milk protein (+36 g/d), lactose (+61 g/d), and total solids (+94 g/d) than cows fed PT6.
33309382	6	110	theme	soybean	980:986	arg1	acids					1069:1073	palm fatty acids	1058:1073	palm fatty acids (FA)	1058:1078	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	110	theme	soybean	980:986	arg1	soap					1050:1053	1.8% calcium soap	1037:1053	1.8% calcium soap of palm fatty acids (FA)	1037:1078	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	110	theme	soybean	980:986	arg1	meal					988:991	9.5% soybean meal	975:991	9.5% soybean meal	975:991	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	110	theme	soybean	980:986	arg1	mix					1106:1108	vitamin mix	1098:1108	vitamin mix	1098:1108	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	110	theme	soybean	980:986	arg1	meal					1031:1034	6.8% low rumen degradability soybean meal	994:1034	6.8% low rumen degradability soybean meal	994:1034	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	110	theme	soybean	980:986	arg1	%					1084:1084	1.7% mineral	1081:1092	1.7% mineral	1081:1092	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	111	theme	vitamin	1098:1104	arg1	meal					988:991	9.5% soybean meal	975:991	9.5% soybean meal	975:991	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	111	theme	vitamin	1098:1104	arg1	mix					1106:1108	vitamin mix	1098:1108	vitamin mix	1098:1108	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	18	112	theme	Lesser	2525:2530	arg1	C18:1					2538:2542	Lesser trans C18:1	2525:2542	Lesser trans C18:1 followed by greater C18:0 concentrations	2525:2583	Lesser trans C18:1 followed by greater C18:0 concentrations were observed for SP12 and PT6 compared with SP6, which is an indication of more complete biohydrogenation in the rumen.
33309382	6	113	theme	9.5	975:977	arg1	%					978:978	%	978:978	%	978:978	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	114	theme	mineral	1086:1092	arg1	meal					988:991	9.5% soybean meal	975:991	9.5% soybean meal	975:991	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	114	theme	mineral	1086:1092	arg1	FA					1076:1077	FA	1076:1077	FA	1076:1077	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	114	theme	mineral	1086:1092	arg1	acids					1069:1073	palm fatty acids	1058:1073	palm fatty acids (FA)	1058:1078	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	114	theme	mineral	1086:1092	arg1	%					1084:1084	1.7% mineral	1081:1092	1.7% mineral	1081:1092	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	1	115	theme	whole-plant	181:191	arg1	WPCS					206:209	WPCS	206:209	WPCS	206:209	Kernel processing and theoretical length of cut (TLOC) of whole-plant corn silage (WPCS) can affect feed intake, digestibility, and performance of dairy cows.
33309382	1	115	theme	whole-plant	181:191	arg1	silage					198:203	whole-plant corn silage	181:203	whole-plant corn silage (WPCS)	181:210	Kernel processing and theoretical length of cut (TLOC) of whole-plant corn silage (WPCS) can affect feed intake, digestibility, and performance of dairy cows.
33309382	17	116	theme	greater	2454:2460	arg1	proportion					2462:2471	greater proportion	2454:2471	greater proportion of milk C14:1 and C16:1	2454:2495	Cows fed SP6 had greater proportion of milk C14:1 and C16:1 compared with PT6 and SP12.
33309382	6	117	theme	48.5	921:924	arg1	%					925:925	%	925:925	%	925:925	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	7	118	dep	%	1203:1203	arg1	protein					1211:1217	crude protein	1205:1217	16.5% crude protein	1199:1217	Nutrient composition of the diets (% of dry matter) was 16.5% crude protein, 28.9% neutral detergent fiber, and 25.4% starch.
33309382	6	119	theme	%	997:997	arg1	meal					1031:1034	6.8% low rumen degradability soybean meal	994:1034	6.8% low rumen degradability soybean meal	994:1034	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	119	theme	%	997:997	arg1	meal					988:991	9.5% soybean meal	975:991	9.5% soybean meal	975:991	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	3	120	dep	harvester	465:473	arg1	forage					458:463	forage	458:463	forage	458:463	The treatments were a pull-type forage harvester without kernel processor set for a 6-mm TLOC (PT6) and a self-propelled forage harvester with kernel processor set for a 6-mm TLOC (SP6), 12-mm TLOC (SP12), and 18-mm TLOC (SP18).
33309382	14	121	theme	lower	2207:2211	arg1	glucose					2220:2226	lower plasma glucose	2207:2226	lower plasma glucose	2207:2226	Cows fed SP18 had lower total-tract starch digestibility and tended to have lower plasma glucose and produce less milk compared with cows fed SP12.
33309382	11	122	theme	components	1755:1764	arg1	yield					1732:1736	increased yield	1722:1736	increased yield of milk and milk components	1722:1764	The mechanism for increased yield of milk and milk components involved greater kernel fragmentation, starch digestibility, and glucose availability for lactose synthesis by the mammary gland.
33309382	11	123	theme	milk	1750:1753	arg1	components					1755:1764	milk and milk components	1741:1764	components	1755:1764	The mechanism for increased yield of milk and milk components involved greater kernel fragmentation, starch digestibility, and glucose availability for lactose synthesis by the mammary gland.
33309382	6	124	theme	dry	958:960	arg1	corn					969:972	15.0% dry ground corn	952:972	15.0% dry ground corn	952:972	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	3	125	theme	self-propelled	532:545	arg1	harvester					554:562	a self-propelled forage harvester	530:562	a self-propelled forage harvester with kernel processor set for a 6-mm TLOC (SP6), 12-mm TLOC (SP12), and 18-mm TLOC (SP18)	530:652	The treatments were a pull-type forage harvester without kernel processor set for a 6-mm TLOC (PT6) and a self-propelled forage harvester with kernel processor set for a 6-mm TLOC (SP6), 12-mm TLOC (SP12), and 18-mm TLOC (SP18).
33309382	11	126	theme	starch	1805:1810	arg1	digestibility					1812:1824	starch digestibility	1805:1824	starch digestibility	1805:1824	The mechanism for increased yield of milk and milk components involved greater kernel fragmentation, starch digestibility, and glucose availability for lactose synthesis by the mammary gland.
33309382	6	127	theme	soybean	1023:1029	arg1	meal					1031:1034	6.8% low rumen degradability soybean meal	994:1034	6.8% low rumen degradability soybean meal	994:1034	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	127	theme	soybean	1023:1029	arg1	meal					988:991	9.5% soybean meal	975:991	9.5% soybean meal	975:991	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	128	theme	dry	1124:1126	arg1	urea					1118:1121	1% urea	1115:1121	1% urea (dry matter basis)	1115:1140	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	128	theme	dry	1124:1126	arg1	basis					1135:1139	dry matter basis	1124:1139	dry matter basis	1124:1139	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	16	129	theme	same	2392:2395	arg1	FA					2397:2398	these same FA	2386:2398	these same FA	2386:2398	Similarly, a reduction of these same FA occurred for SP12 compared with SP6.
33309382	18	130	theme	complete	2666:2673	arg1	biohydrogenation					2675:2690	more complete biohydrogenation	2661:2690	more complete biohydrogenation	2661:2690	Lesser trans C18:1 followed by greater C18:0 concentrations were observed for SP12 and PT6 compared with SP6, which is an indication of more complete biohydrogenation in the rumen.
33309382	11	131	theme	glucose	1831:1837	arg1	availability					1839:1850	glucose availability	1831:1850	glucose availability	1831:1850	The mechanism for increased yield of milk and milk components involved greater kernel fragmentation, starch digestibility, and glucose availability for lactose synthesis by the mammary gland.
33309382	3	132	dep	harvester	554:562	arg1	forage					547:552	forage	547:552	forage	547:552	The treatments were a pull-type forage harvester without kernel processor set for a 6-mm TLOC (PT6) and a self-propelled forage harvester with kernel processor set for a 6-mm TLOC (SP6), 12-mm TLOC (SP12), and 18-mm TLOC (SP18).
33309382	5	133	with	squares	840:846	arg1	periods					858:864	24-d periods	853:864	24-d periods (18 d of adaptation)	853:885	Twenty-four Holstein cows (139 ± 63 d in milk) were blocked and assigned to six 4 × 4 Latin squares with 24-d periods (18 d of adaptation).
33309382	6	134	theme	6.8	994:996	arg1	%					997:997	%	997:997	%	997:997	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	5	135	from	d	784:784	arg1	milk					789:792	milk	789:792	milk	789:792	Twenty-four Holstein cows (139 ± 63 d in milk) were blocked and assigned to six 4 × 4 Latin squares with 24-d periods (18 d of adaptation).
33309382	5	136	theme	Latin	834:838	arg1	squares					840:846	4 × 4 Latin squares	828:846	six 4 × 4 Latin squares with 24-d periods (18 d of adaptation)	824:885	Twenty-four Holstein cows (139 ± 63 d in milk) were blocked and assigned to six 4 × 4 Latin squares with 24-d periods (18 d of adaptation).
33309382	1	137	theme	theoretical	145:155	arg1	length					157:162	theoretical length	145:162	theoretical length	145:162	Kernel processing and theoretical length of cut (TLOC) of whole-plant corn silage (WPCS) can affect feed intake, digestibility, and performance of dairy cows.
33309382	7	138	theme	detergent	1234:1242	arg1	fiber					1244:1248	neutral detergent fiber	1226:1248	28.9% neutral detergent fiber	1220:1248	Nutrient composition of the diets (% of dry matter) was 16.5% crude protein, 28.9% neutral detergent fiber, and 25.4% starch.
33309382	6	139	theme	%	978:978	arg1	acids					1069:1073	palm fatty acids	1058:1073	palm fatty acids (FA)	1058:1078	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	139	theme	%	978:978	arg1	soap					1050:1053	1.8% calcium soap	1037:1053	1.8% calcium soap of palm fatty acids (FA)	1037:1078	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	139	theme	%	978:978	arg1	meal					988:991	9.5% soybean meal	975:991	9.5% soybean meal	975:991	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	139	theme	%	978:978	arg1	mix					1106:1108	vitamin mix	1098:1108	vitamin mix	1098:1108	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	139	theme	%	978:978	arg1	meal					1031:1034	6.8% low rumen degradability soybean meal	994:1034	6.8% low rumen degradability soybean meal	994:1034	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	139	theme	%	978:978	arg1	%					1084:1084	1.7% mineral	1081:1092	1.7% mineral	1081:1092	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	14	140	contain	had	2145:2147	arg1	Cows					2131:2134	Cows	2131:2134	Cows fed SP18	2131:2143	Cows fed SP18 had lower total-tract starch digestibility and tended to have lower plasma glucose and produce less milk compared with cows fed SP12.
33309382	14	140	contain	had	2145:2147	arg2	digestibility					2174:2186	lower total-tract starch digestibility	2149:2186	lower total-tract starch digestibility	2149:2186	Cows fed SP18 had lower total-tract starch digestibility and tended to have lower plasma glucose and produce less milk compared with cows fed SP12.
33309382	11	141	theme	increased	1722:1730	arg1	yield					1732:1736	increased yield	1722:1736	increased yield of milk and milk components	1722:1764	The mechanism for increased yield of milk and milk components involved greater kernel fragmentation, starch digestibility, and glucose availability for lactose synthesis by the mammary gland.
33309382	13	142	theme	chewing	2117:2123	arg1	time					2125:2128	greater chewing time	2109:2128	greater chewing time	2109:2128	Milk yield was similar for SP6 and SP12, but SP12 cows tended to have less serum amyloid A with greater chewing time.
33309382	3	143	with	TLOC	515:518	arg1	processor					576:584	kernel processor	569:584	kernel processor set for a 6-mm TLOC (SP6), 12-mm TLOC (SP12), and 18-mm TLOC (SP18)	569:652	The treatments were a pull-type forage harvester without kernel processor set for a 6-mm TLOC (PT6) and a self-propelled forage harvester with kernel processor set for a 6-mm TLOC (SP6), 12-mm TLOC (SP12), and 18-mm TLOC (SP18).
33309382	19	144	with	hybrids	2860:2866	arg1	endosperm					2882:2890	vitreous endosperm	2873:2890	vitreous endosperm	2873:2890	Under the conditions of this study, the use of a self-propelled forage harvester with kernel processing set for a 12-mm TLOC is recommended for WPCS from hybrids with vitreous endosperm.
33309382	0	145	theme	dairy	100:104	arg1	performance					110:120	dairy cow performance	100:120	dairy cow performance	100:120	Effect of kernel processing and particle size of whole-plant corn silage with vitreous endosperm on dairy cow performance.
33309382	13	146	theme	greater	2109:2115	arg1	time					2125:2128	greater chewing time	2109:2128	greater chewing time	2109:2128	Milk yield was similar for SP6 and SP12, but SP12 cows tended to have less serum amyloid A with greater chewing time.
33309382	9	147	theme	greater	1470:1476	arg1	yield					1483:1487	1.2 kg/d greater milk yield	1461:1487	1.2 kg/d greater milk yield	1461:1487	Cows fed SP6 produced 1.2 kg/d greater milk yield with no changes in dry matter intake, resulting in greater feed efficiency compared with PT6.
33309382	2	148	theme	lactating	330:338	arg1	cows					346:349	lactating dairy cows	330:349	lactating dairy cows	330:349	The objective of this study was to evaluate for lactating dairy cows the effects of kernel processing and TLOC of WPCS with vitreous endosperm.
33309382	6	149	theme	palm	1058:1061	arg1	FA					1076:1077	FA	1076:1077	FA	1076:1077	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	149	theme	palm	1058:1061	arg1	acids					1069:1073	palm fatty acids	1058:1073	palm fatty acids (FA)	1058:1078	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	5	150	theme	×	830:830	arg1	squares					840:846	4 × 4 Latin squares	828:846	six 4 × 4 Latin squares with 24-d periods (18 d of adaptation)	824:885	Twenty-four Holstein cows (139 ± 63 d in milk) were blocked and assigned to six 4 × 4 Latin squares with 24-d periods (18 d of adaptation).
33309382	7	151	theme	crude	1205:1209	arg1	protein					1211:1217	crude protein	1205:1217	16.5% crude protein	1199:1217	Nutrient composition of the diets (% of dry matter) was 16.5% crude protein, 28.9% neutral detergent fiber, and 25.4% starch.
33309382	12	152	theme	chewing	1928:1934	arg1	time					1936:1939	lower chewing time	1922:1939	lower chewing time	1922:1939	However, cows fed SP6 had lower chewing time and tended to have greater levels of serum amyloid A compared with PT6.
33309382	0	153	theme	processing	17:26	arg1	Effect					0:5	Effect	0:5	Effect of kernel processing and particle size of whole-plant corn silage with vitreous endosperm on dairy cow performance.	0:121	Effect of kernel processing and particle size of whole-plant corn silage with vitreous endosperm on dairy cow performance.
33309382	8	154	theme	kernel	1339:1344	arg1	processing					1346:1355	kernel processing	1339:1355	kernel processing (PT6 vs. SP6)	1339:1369	Three orthogonal contrasts were used to compare treatments: effect of kernel processing (PT6 vs. SP6) and effect of TLOC (particle size; SP6 vs. SP12 and SP12 vs. SP18).
33309382	2	155	theme	kernel	366:371	arg1	processing					373:382	kernel processing	366:382	kernel processing	366:382	The objective of this study was to evaluate for lactating dairy cows the effects of kernel processing and TLOC of WPCS with vitreous endosperm.
33309382	3	156	theme	kernel	569:574	arg1	processor					576:584	kernel processor	569:584	kernel processor set for a 6-mm TLOC (SP6), 12-mm TLOC (SP12), and 18-mm TLOC (SP18)	569:652	The treatments were a pull-type forage harvester without kernel processor set for a 6-mm TLOC (PT6) and a self-propelled forage harvester with kernel processor set for a 6-mm TLOC (SP6), 12-mm TLOC (SP12), and 18-mm TLOC (SP18).
33309382	13	157	theme	Milk	2013:2016	arg1	yield					2018:2022	Milk yield	2013:2022	Milk yield	2013:2022	Milk yield was similar for SP6 and SP12, but SP12 cows tended to have less serum amyloid A with greater chewing time.
33309382	6	158	theme	%	1040:1040	arg1	soap					1050:1053	1.8% calcium soap	1037:1053	1.8% calcium soap of palm fatty acids (FA)	1037:1078	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	6	158	theme	%	1040:1040	arg1	meal					988:991	9.5% soybean meal	975:991	9.5% soybean meal	975:991	Diets were formulated to contain 48.5% WPCS, 15.5% citrus pulp, 15.0% dry ground corn, 9.5% soybean meal, 6.8% low rumen degradability soybean meal, 1.8% calcium soap of palm fatty acids (FA), 1.7% mineral and vitamin mix, and 1% urea (dry matter basis).
33309382	9	159	theme	milk	1478:1481	arg1	yield					1483:1487	1.2 kg/d greater milk yield	1461:1487	1.2 kg/d greater milk yield	1461:1487	Cows fed SP6 produced 1.2 kg/d greater milk yield with no changes in dry matter intake, resulting in greater feed efficiency compared with PT6.
33309382	0	160	with	processing	17:26	arg1	endosperm					87:95	vitreous endosperm	78:95	vitreous endosperm	78:95	Effect of kernel processing and particle size of whole-plant corn silage with vitreous endosperm on dairy cow performance.
33309382	8	161	dep	TLOC	1385:1388	arg1	size					1400:1403	particle size	1391:1403	particle size	1391:1403	Three orthogonal contrasts were used to compare treatments: effect of kernel processing (PT6 vs. SP6) and effect of TLOC (particle size; SP6 vs. SP12 and SP12 vs. SP18).
33309382	9	162	theme	matter	1512:1517	arg1	intake					1519:1524	dry matter intake	1508:1524	dry matter intake	1508:1524	Cows fed SP6 produced 1.2 kg/d greater milk yield with no changes in dry matter intake, resulting in greater feed efficiency compared with PT6.
33309382	4	163	theme	WPCS	680:683	arg1	scores					666:671	Processing scores	655:671	Processing scores of the WPCS	655:683	Processing scores of the WPCS were 32.1% (PT6), 53.9% (SP6), 49.0% (SP12), and 40.1% (SP18).
33309382	16	164	theme	FA	2397:2398	arg1	reduction					2373:2381	a reduction	2371:2381	a reduction of these same FA	2371:2398	Similarly, a reduction of these same FA occurred for SP12 compared with SP6.
33309382	1	165	theme	cows	276:279	arg1	digestibility					236:248	digestibility	236:248	digestibility	236:248	Kernel processing and theoretical length of cut (TLOC) of whole-plant corn silage (WPCS) can affect feed intake, digestibility, and performance of dairy cows.
33309382	1	165	theme	cows	276:279	arg1	performance					255:265	performance	255:265	performance	255:265	Kernel processing and theoretical length of cut (TLOC) of whole-plant corn silage (WPCS) can affect feed intake, digestibility, and performance of dairy cows.
33309382	1	165	theme	cows	276:279	arg1	intake					228:233	feed intake	223:233	feed intake	223:233	Kernel processing and theoretical length of cut (TLOC) of whole-plant corn silage (WPCS) can affect feed intake, digestibility, and performance of dairy cows.
33309382	19	166	from	hybrids	2860:2866	arg1	WPCS					2850:2853	WPCS	2850:2853	WPCS from hybrids with vitreous endosperm	2850:2890	Under the conditions of this study, the use of a self-propelled forage harvester with kernel processing set for a 12-mm TLOC is recommended for WPCS from hybrids with vitreous endosperm.
33309382	15	167	theme	odd-chain	2324:2332	arg1	concentration					2337:2349	linear odd-chain FA concentration	2317:2349	linear odd-chain FA concentration	2317:2349	Compared with PT6, feeding SP6 raised linear odd-chain FA concentration in milk.
33309382	14	168	theme	starch	2167:2172	arg1	digestibility					2174:2186	lower total-tract starch digestibility	2149:2186	lower total-tract starch digestibility	2149:2186	Cows fed SP18 had lower total-tract starch digestibility and tended to have lower plasma glucose and produce less milk compared with cows fed SP12.
33309382	12	169	theme	amyloid	1984:1990	arg1	A					1992:1992	serum amyloid A	1978:1992	serum amyloid A	1978:1992	However, cows fed SP6 had lower chewing time and tended to have greater levels of serum amyloid A compared with PT6.
33309382	11	170	theme	greater	1775:1781	arg1	fragmentation					1790:1802	greater kernel fragmentation	1775:1802	greater kernel fragmentation	1775:1802	The mechanism for increased yield of milk and milk components involved greater kernel fragmentation, starch digestibility, and glucose availability for lactose synthesis by the mammary gland.
33309382	5	171	theme	Holstein	760:767	arg1	cows					769:772	Twenty-four Holstein cows	748:772	Twenty-four Holstein cows (139 ± 63 d in milk)	748:793	Twenty-four Holstein cows (139 ± 63 d in milk) were blocked and assigned to six 4 × 4 Latin squares with 24-d periods (18 d of adaptation).
33309382	5	171	theme	Holstein	760:767	arg1	±					779:779	139 ± 63 d in milk	775:792	139 ± 63 d in milk	775:792	Twenty-four Holstein cows (139 ± 63 d in milk) were blocked and assigned to six 4 × 4 Latin squares with 24-d periods (18 d of adaptation).
33309382	8	172	dep	orthogonal	1275:1284	arg1	contrasts					1286:1294	contrasts	1286:1294	contrasts	1286:1294	Three orthogonal contrasts were used to compare treatments: effect of kernel processing (PT6 vs. SP6) and effect of TLOC (particle size; SP6 vs. SP12 and SP12 vs. SP18).
33309382	4	173	theme	Processing	655:664	arg1	scores					666:671	Processing scores	655:671	Processing scores of the WPCS	655:683	Processing scores of the WPCS were 32.1% (PT6), 53.9% (SP6), 49.0% (SP12), and 40.1% (SP18).
32770751	4	0	theme	pectin	693:698	arg1	mutants					725:731	pectin acetylesterase atpae11.1 mutants	693:731	pectin acetylesterase atpae11.1 mutants in comparison to wild-type Col-0	693:764	In this work, we tested for the first time how cell wall dynamics affect photosynthetic properties in Arabidopsis thaliana pectin methylesterase atpme17.2 and pectin acetylesterase atpae11.1 mutants in comparison to wild-type Col-0.
32770751	1	1	theme	complex	146:152	arg1	wall					136:139	The cell wall	127:139	The cell wall	127:139	The cell wall is a complex and dynamic structure that determines plants' performance by constant remodeling of its compounds.
32770751	1	1	theme	complex	146:152	arg1	structure					166:174	a complex and dynamic structure	144:174	a complex and dynamic structure that determines plants' performance by constant remodeling of its compounds	144:250	The cell wall is a complex and dynamic structure that determines plants' performance by constant remodeling of its compounds.
32770751	7	2	dep	assimilation	1202:1213	arg1	AN					1216:1217	AN	1216:1217	AN	1216:1217	Additionally, physiological characterization revealed that mutants presented a decreased net CO2 assimilation (AN ) because of reductions in both stomatal (gs ) and mesophyll conductances (gm ).
32770751	1	3	theme	compounds	242:250	arg1	remodeling					224:233	constant remodeling	215:233	constant remodeling of its compounds	215:250	The cell wall is a complex and dynamic structure that determines plants' performance by constant remodeling of its compounds.
32770751	0	4	from	photosynthesis	8:21	arg1	mutants					71:77	atpae11.1 mutants	61:77	atpae11.1 mutants	61:77	Reduced photosynthesis in Arabidopsis thaliana atpme17.2 and atpae11.1 mutants is associated to altered cell wall composition.
32770751	0	4	from	photosynthesis	8:21	arg1	thaliana					38:45	Arabidopsis thaliana atpme17.2	26:55	Arabidopsis thaliana atpme17.2	26:55	Reduced photosynthesis in Arabidopsis thaliana atpme17.2 and atpae11.1 mutants is associated to altered cell wall composition.
32770751	5	5	theme	neutral	919:925	arg1	sugars					927:932	cell wall non-cellulosic neutral sugars	894:932	cell wall non-cellulosic neutral sugars contents	894:941	Our results showed maintained PRE activities comparing mutants with wild-type and no significant differences in cellulose, but cell wall non-cellulosic neutral sugars contents changed.
32770751	5	6	theme	sugars	927:932	arg1	contents					934:941	cell wall non-cellulosic neutral sugars contents	894:941	cell wall non-cellulosic neutral sugars contents	894:941	Our results showed maintained PRE activities comparing mutants with wild-type and no significant differences in cellulose, but cell wall non-cellulosic neutral sugars contents changed.
32770751	8	7	theme	wall	1360:1363	arg1	modifications					1365:1377	atpme17.2 and atpae11.1 cell wall modifications	1331:1377	modifications	1365:1377	Thus, our results suggest that atpme17.2 and atpae11.1 cell wall modifications due to genetic alterations could play a significant role in determining photosynthesis.
32770751	7	8	theme	physiological	1119:1131	arg1	characterization					1133:1148	physiological characterization	1119:1148	physiological characterization	1119:1148	Additionally, physiological characterization revealed that mutants presented a decreased net CO2 assimilation (AN ) because of reductions in both stomatal (gs ) and mesophyll conductances (gm ).
32770751	0	9	theme	cell	104:107	arg1	composition					114:124	altered cell wall composition	96:124	altered cell wall composition	96:124	Reduced photosynthesis in Arabidopsis thaliana atpme17.2 and atpae11.1 mutants is associated to altered cell wall composition.
32770751	2	10	theme	load-bearing	285:296	arg1	component					298:306	its major load-bearing component	275:306	its major load-bearing component	275:306	Although cellulose is its major load-bearing component, pectins are crucial to determine wall characteristics.
32770751	4	11	theme	Arabidopsis	636:646	arg1	methylesterase					664:677	Arabidopsis thaliana pectin methylesterase atpme17.2	636:687	Arabidopsis thaliana pectin methylesterase atpme17.2	636:687	In this work, we tested for the first time how cell wall dynamics affect photosynthetic properties in Arabidopsis thaliana pectin methylesterase atpme17.2 and pectin acetylesterase atpae11.1 mutants in comparison to wild-type Col-0.
32770751	4	12	theme	first	566:570	arg1	time					572:575	the first time	562:575	the first time	562:575	In this work, we tested for the first time how cell wall dynamics affect photosynthetic properties in Arabidopsis thaliana pectin methylesterase atpme17.2 and pectin acetylesterase atpae11.1 mutants in comparison to wild-type Col-0.
32770751	0	13	theme	altered	96:102	arg1	composition					114:124	altered cell wall composition	96:124	altered cell wall composition	96:124	Reduced photosynthesis in Arabidopsis thaliana atpme17.2 and atpae11.1 mutants is associated to altered cell wall composition.
32770751	2	14	theme	major	279:283	arg1	component					298:306	its major load-bearing component	275:306	its major load-bearing component	275:306	Although cellulose is its major load-bearing component, pectins are crucial to determine wall characteristics.
32770751	3	15	theme	wall	483:486	arg1	compounds					488:496	cell wall compounds	478:496	cell wall compounds	478:496	Changes in pectin physicochemical properties, due to pectin remodeling enzymes (PRE), induce the rearrangement of cell wall compounds, thus, modifying wall architecture.
32770751	6	16	theme	wall	1055:1058	arg1	proportion					1060:1069	the pectin cell wall proportion	1039:1069	the pectin cell wall proportion	1039:1069	Particularly, the amount of galacturonic acid (GalA) - which represents to some extent the pectin cell wall proportion - was reduced in the two mutants.
32770751	3	17	theme	wall	515:518	arg1	architecture					520:531	wall architecture	515:531	wall architecture	515:531	Changes in pectin physicochemical properties, due to pectin remodeling enzymes (PRE), induce the rearrangement of cell wall compounds, thus, modifying wall architecture.
32770751	1	18	theme	dynamic	158:164	arg1	wall					136:139	The cell wall	127:139	The cell wall	127:139	The cell wall is a complex and dynamic structure that determines plants' performance by constant remodeling of its compounds.
32770751	1	18	theme	dynamic	158:164	arg1	structure					166:174	a complex and dynamic structure	144:174	a complex and dynamic structure that determines plants' performance by constant remodeling of its compounds	144:250	The cell wall is a complex and dynamic structure that determines plants' performance by constant remodeling of its compounds.
32770751	0	19	theme	wall	109:112	arg1	composition					114:124	altered cell wall composition	96:124	altered cell wall composition	96:124	Reduced photosynthesis in Arabidopsis thaliana atpme17.2 and atpae11.1 mutants is associated to altered cell wall composition.
32770751	5	20	from	mutants	822:828	arg1	cellulose					879:887	cellulose	879:887	cellulose	879:887	Our results showed maintained PRE activities comparing mutants with wild-type and no significant differences in cellulose, but cell wall non-cellulosic neutral sugars contents changed.
32770751	5	21	theme	maintained	786:795	arg1	activities					801:810	maintained PRE activities	786:810	maintained PRE activities comparing mutants with wild-type and no significant differences in cellulose	786:887	Our results showed maintained PRE activities comparing mutants with wild-type and no significant differences in cellulose, but cell wall non-cellulosic neutral sugars contents changed.
32770751	7	22	theme	CO2	1198:1200	arg1	assimilation					1202:1213	a decreased net CO2 assimilation	1182:1213	a decreased net CO2 assimilation (AN )	1182:1219	Additionally, physiological characterization revealed that mutants presented a decreased net CO2 assimilation (AN ) because of reductions in both stomatal (gs ) and mesophyll conductances (gm ).
32770751	7	23	theme	net	1194:1196	arg1	assimilation					1202:1213	a decreased net CO2 assimilation	1182:1213	a decreased net CO2 assimilation (AN )	1182:1219	Additionally, physiological characterization revealed that mutants presented a decreased net CO2 assimilation (AN ) because of reductions in both stomatal (gs ) and mesophyll conductances (gm ).
32770751	6	24	theme	galacturonic	980:991	arg1	acid					993:996	galacturonic acid	980:996	galacturonic acid (GalA) - which represents to some extent the pectin cell wall proportion -	980:1071	Particularly, the amount of galacturonic acid (GalA) - which represents to some extent the pectin cell wall proportion - was reduced in the two mutants.
32770751	6	24	theme	galacturonic	980:991	arg1	GalA					999:1002	GalA	999:1002	GalA	999:1002	Particularly, the amount of galacturonic acid (GalA) - which represents to some extent the pectin cell wall proportion - was reduced in the two mutants.
32770751	8	25	theme	cell	1355:1358	arg1	modifications					1365:1377	atpme17.2 and atpae11.1 cell wall modifications	1331:1377	modifications	1365:1377	Thus, our results suggest that atpme17.2 and atpae11.1 cell wall modifications due to genetic alterations could play a significant role in determining photosynthesis.
32770751	4	26	theme	pectin	657:662	arg1	methylesterase					664:677	Arabidopsis thaliana pectin methylesterase atpme17.2	636:687	Arabidopsis thaliana pectin methylesterase atpme17.2	636:687	In this work, we tested for the first time how cell wall dynamics affect photosynthetic properties in Arabidopsis thaliana pectin methylesterase atpme17.2 and pectin acetylesterase atpae11.1 mutants in comparison to wild-type Col-0.
32770751	0	27	theme	Reduced	0:6	arg1	photosynthesis					8:21	Reduced photosynthesis	0:21	Reduced photosynthesis in Arabidopsis thaliana atpme17.2 and atpae11.1 mutants	0:77	Reduced photosynthesis in Arabidopsis thaliana atpme17.2 and atpae11.1 mutants is associated to altered cell wall composition.
32770751	8	28	theme	atpae11.1	1345:1353	arg1	modifications					1365:1377	atpme17.2 and atpae11.1 cell wall modifications	1331:1377	modifications	1365:1377	Thus, our results suggest that atpme17.2 and atpae11.1 cell wall modifications due to genetic alterations could play a significant role in determining photosynthesis.
32770751	4	29	from	properties	622:631	arg1	methylesterase					664:677	Arabidopsis thaliana pectin methylesterase atpme17.2	636:687	Arabidopsis thaliana pectin methylesterase atpme17.2	636:687	In this work, we tested for the first time how cell wall dynamics affect photosynthetic properties in Arabidopsis thaliana pectin methylesterase atpme17.2 and pectin acetylesterase atpae11.1 mutants in comparison to wild-type Col-0.
32770751	4	29	from	properties	622:631	arg1	mutants					725:731	pectin acetylesterase atpae11.1 mutants	693:731	pectin acetylesterase atpae11.1 mutants in comparison to wild-type Col-0	693:764	In this work, we tested for the first time how cell wall dynamics affect photosynthetic properties in Arabidopsis thaliana pectin methylesterase atpme17.2 and pectin acetylesterase atpae11.1 mutants in comparison to wild-type Col-0.
32770751	5	30	theme	cell	894:897	arg1	sugars					927:932	cell wall non-cellulosic neutral sugars	894:932	cell wall non-cellulosic neutral sugars contents	894:941	Our results showed maintained PRE activities comparing mutants with wild-type and no significant differences in cellulose, but cell wall non-cellulosic neutral sugars contents changed.
32770751	3	31	theme	pectin	375:380	arg1	properties					398:407	pectin physicochemical properties	375:407	pectin physicochemical properties	375:407	Changes in pectin physicochemical properties, due to pectin remodeling enzymes (PRE), induce the rearrangement of cell wall compounds, thus, modifying wall architecture.
32770751	8	32	theme	significant	1419:1429	arg1	role					1431:1434	a significant role	1417:1434	a significant role	1417:1434	Thus, our results suggest that atpme17.2 and atpae11.1 cell wall modifications due to genetic alterations could play a significant role in determining photosynthesis.
32770751	5	33	with	differences	864:874	arg1	wild-type					835:843	wild-type	835:843	wild-type	835:843	Our results showed maintained PRE activities comparing mutants with wild-type and no significant differences in cellulose, but cell wall non-cellulosic neutral sugars contents changed.
32770751	5	34	with	mutants	822:828	arg1	wild-type					835:843	wild-type	835:843	wild-type	835:843	Our results showed maintained PRE activities comparing mutants with wild-type and no significant differences in cellulose, but cell wall non-cellulosic neutral sugars contents changed.
32770751	3	35	theme	physicochemical	382:396	arg1	properties					398:407	pectin physicochemical properties	375:407	pectin physicochemical properties	375:407	Changes in pectin physicochemical properties, due to pectin remodeling enzymes (PRE), induce the rearrangement of cell wall compounds, thus, modifying wall architecture.
32770751	6	36	theme	acid	993:996	arg1	amount					970:975	the amount	966:975	the amount of galacturonic acid (GalA) - which represents to some extent the pectin cell wall proportion -	966:1071	Particularly, the amount of galacturonic acid (GalA) - which represents to some extent the pectin cell wall proportion - was reduced in the two mutants.
32770751	6	36	theme	acid	993:996	arg1	acid					993:996	galacturonic acid	980:996	galacturonic acid (GalA) - which represents to some extent the pectin cell wall proportion -	980:1071	Particularly, the amount of galacturonic acid (GalA) - which represents to some extent the pectin cell wall proportion - was reduced in the two mutants.
32770751	6	36	theme	acid	993:996	arg1	GalA					999:1002	GalA	999:1002	GalA	999:1002	Particularly, the amount of galacturonic acid (GalA) - which represents to some extent the pectin cell wall proportion - was reduced in the two mutants.
32770751	4	37	theme	wild-type	750:758	arg1	Col-0					760:764	wild-type Col-0	750:764	wild-type Col-0	750:764	In this work, we tested for the first time how cell wall dynamics affect photosynthetic properties in Arabidopsis thaliana pectin methylesterase atpme17.2 and pectin acetylesterase atpae11.1 mutants in comparison to wild-type Col-0.
32770751	5	38	theme	significant	852:862	arg1	differences					864:874	no significant differences	849:874	no significant differences in cellulose	849:887	Our results showed maintained PRE activities comparing mutants with wild-type and no significant differences in cellulose, but cell wall non-cellulosic neutral sugars contents changed.
32770751	3	39	theme	pectin	417:422	arg1	PRE					444:446	PRE	444:446	PRE	444:446	Changes in pectin physicochemical properties, due to pectin remodeling enzymes (PRE), induce the rearrangement of cell wall compounds, thus, modifying wall architecture.
32770751	3	39	theme	pectin	417:422	arg1	enzymes					435:441	pectin remodeling enzymes	417:441	pectin remodeling enzymes (PRE)	417:447	Changes in pectin physicochemical properties, due to pectin remodeling enzymes (PRE), induce the rearrangement of cell wall compounds, thus, modifying wall architecture.
32770751	4	40	theme	acetylesterase	700:713	arg1	mutants					725:731	pectin acetylesterase atpae11.1 mutants	693:731	pectin acetylesterase atpae11.1 mutants in comparison to wild-type Col-0	693:764	In this work, we tested for the first time how cell wall dynamics affect photosynthetic properties in Arabidopsis thaliana pectin methylesterase atpme17.2 and pectin acetylesterase atpae11.1 mutants in comparison to wild-type Col-0.
32770751	6	41	theme	cell	1050:1053	arg1	proportion					1060:1069	the pectin cell wall proportion	1039:1069	the pectin cell wall proportion	1039:1069	Particularly, the amount of galacturonic acid (GalA) - which represents to some extent the pectin cell wall proportion - was reduced in the two mutants.
32770751	3	42	theme	remodeling	424:433	arg1	PRE					444:446	PRE	444:446	PRE	444:446	Changes in pectin physicochemical properties, due to pectin remodeling enzymes (PRE), induce the rearrangement of cell wall compounds, thus, modifying wall architecture.
32770751	3	42	theme	remodeling	424:433	arg1	enzymes					435:441	pectin remodeling enzymes	417:441	pectin remodeling enzymes (PRE)	417:447	Changes in pectin physicochemical properties, due to pectin remodeling enzymes (PRE), induce the rearrangement of cell wall compounds, thus, modifying wall architecture.
32770751	5	43	theme	PRE	797:799	arg1	activities					801:810	maintained PRE activities	786:810	maintained PRE activities comparing mutants with wild-type and no significant differences in cellulose	786:887	Our results showed maintained PRE activities comparing mutants with wild-type and no significant differences in cellulose, but cell wall non-cellulosic neutral sugars contents changed.
32770751	3	44	from	Changes	364:370	arg1	properties					398:407	pectin physicochemical properties	375:407	pectin physicochemical properties	375:407	Changes in pectin physicochemical properties, due to pectin remodeling enzymes (PRE), induce the rearrangement of cell wall compounds, thus, modifying wall architecture.
32770751	0	45	theme	Arabidopsis	26:36	arg1	thaliana					38:45	Arabidopsis thaliana atpme17.2	26:55	Arabidopsis thaliana atpme17.2	26:55	Reduced photosynthesis in Arabidopsis thaliana atpme17.2 and atpae11.1 mutants is associated to altered cell wall composition.
32770751	5	46	from	differences	864:874	arg1	cellulose					879:887	cellulose	879:887	cellulose	879:887	Our results showed maintained PRE activities comparing mutants with wild-type and no significant differences in cellulose, but cell wall non-cellulosic neutral sugars contents changed.
32770751	4	47	theme	photosynthetic	607:620	arg1	properties					622:631	photosynthetic properties	607:631	photosynthetic properties in Arabidopsis thaliana pectin methylesterase atpme17.2 and pectin acetylesterase atpae11.1 mutants in comparison to wild-type Col-0	607:764	In this work, we tested for the first time how cell wall dynamics affect photosynthetic properties in Arabidopsis thaliana pectin methylesterase atpme17.2 and pectin acetylesterase atpae11.1 mutants in comparison to wild-type Col-0.
32770751	0	48	theme	atpae11.1	61:69	arg1	mutants					71:77	atpae11.1 mutants	61:77	atpae11.1 mutants	61:77	Reduced photosynthesis in Arabidopsis thaliana atpme17.2 and atpae11.1 mutants is associated to altered cell wall composition.
32770751	6	49	theme	pectin	1043:1048	arg1	proportion					1060:1069	the pectin cell wall proportion	1039:1069	the pectin cell wall proportion	1039:1069	Particularly, the amount of galacturonic acid (GalA) - which represents to some extent the pectin cell wall proportion - was reduced in the two mutants.
32770751	2	50	theme	wall	342:345	arg1	characteristics					347:361	wall characteristics	342:361	wall characteristics	342:361	Although cellulose is its major load-bearing component, pectins are crucial to determine wall characteristics.
32770751	6	51	dep	acid	993:996	arg1	represents					1013:1022	represents	1013:1022	represents to some extent the pectin cell wall proportion	1013:1069	Particularly, the amount of galacturonic acid (GalA) - which represents to some extent the pectin cell wall proportion - was reduced in the two mutants.
32770751	8	52	theme	genetic	1386:1392	arg1	alterations					1394:1404	genetic alterations	1386:1404	genetic alterations	1386:1404	Thus, our results suggest that atpme17.2 and atpae11.1 cell wall modifications due to genetic alterations could play a significant role in determining photosynthesis.
32770751	7	53	theme	mesophyll	1270:1278	arg1	gm					1294:1295	gm	1294:1295	gm	1294:1295	Additionally, physiological characterization revealed that mutants presented a decreased net CO2 assimilation (AN ) because of reductions in both stomatal (gs ) and mesophyll conductances (gm ).
32770751	7	53	theme	mesophyll	1270:1278	arg1	conductances					1280:1291	mesophyll conductances	1270:1291	mesophyll conductances (gm )	1270:1297	Additionally, physiological characterization revealed that mutants presented a decreased net CO2 assimilation (AN ) because of reductions in both stomatal (gs ) and mesophyll conductances (gm ).
32770751	3	54	theme	compounds	488:496	arg1	rearrangement					461:473	the rearrangement	457:473	the rearrangement of cell wall compounds	457:496	Changes in pectin physicochemical properties, due to pectin remodeling enzymes (PRE), induce the rearrangement of cell wall compounds, thus, modifying wall architecture.
32770751	4	55	theme	atpae11.1	715:723	arg1	mutants					725:731	pectin acetylesterase atpae11.1 mutants	693:731	pectin acetylesterase atpae11.1 mutants in comparison to wild-type Col-0	693:764	In this work, we tested for the first time how cell wall dynamics affect photosynthetic properties in Arabidopsis thaliana pectin methylesterase atpme17.2 and pectin acetylesterase atpae11.1 mutants in comparison to wild-type Col-0.
32770751	1	56	theme	cell	131:134	arg1	wall					136:139	The cell wall	127:139	The cell wall	127:139	The cell wall is a complex and dynamic structure that determines plants' performance by constant remodeling of its compounds.
32770751	1	56	theme	cell	131:134	arg1	structure					166:174	a complex and dynamic structure	144:174	a complex and dynamic structure that determines plants' performance by constant remodeling of its compounds	144:250	The cell wall is a complex and dynamic structure that determines plants' performance by constant remodeling of its compounds.
32770751	4	57	from	mutants	725:731	arg1	comparison					736:745	comparison	736:745	comparison to wild-type Col-0	736:764	In this work, we tested for the first time how cell wall dynamics affect photosynthetic properties in Arabidopsis thaliana pectin methylesterase atpme17.2 and pectin acetylesterase atpae11.1 mutants in comparison to wild-type Col-0.
32770751	1	58	theme	constant	215:222	arg1	remodeling					224:233	constant remodeling	215:233	constant remodeling of its compounds	215:250	The cell wall is a complex and dynamic structure that determines plants' performance by constant remodeling of its compounds.
32770751	8	59	theme	atpme17.2	1331:1339	arg1	modifications					1365:1377	atpme17.2 and atpae11.1 cell wall modifications	1331:1377	modifications	1365:1377	Thus, our results suggest that atpme17.2 and atpae11.1 cell wall modifications due to genetic alterations could play a significant role in determining photosynthesis.
32770751	7	60	from	reductions	1232:1241	arg1	gm					1294:1295	gm	1294:1295	gm	1294:1295	Additionally, physiological characterization revealed that mutants presented a decreased net CO2 assimilation (AN ) because of reductions in both stomatal (gs ) and mesophyll conductances (gm ).
32770751	7	60	from	reductions	1232:1241	arg1	gs					1261:1262	gs	1261:1262	gs	1261:1262	Additionally, physiological characterization revealed that mutants presented a decreased net CO2 assimilation (AN ) because of reductions in both stomatal (gs ) and mesophyll conductances (gm ).
32770751	7	60	from	reductions	1232:1241	arg1	stomatal					1251:1258	stomatal	1251:1258	stomatal (gs )	1251:1264	Additionally, physiological characterization revealed that mutants presented a decreased net CO2 assimilation (AN ) because of reductions in both stomatal (gs ) and mesophyll conductances (gm ).
32770751	7	60	from	reductions	1232:1241	arg1	conductances					1280:1291	mesophyll conductances	1270:1291	mesophyll conductances (gm )	1270:1297	Additionally, physiological characterization revealed that mutants presented a decreased net CO2 assimilation (AN ) because of reductions in both stomatal (gs ) and mesophyll conductances (gm ).
32770751	3	61	theme	cell	478:481	arg1	compounds					488:496	cell wall compounds	478:496	cell wall compounds	478:496	Changes in pectin physicochemical properties, due to pectin remodeling enzymes (PRE), induce the rearrangement of cell wall compounds, thus, modifying wall architecture.
32770751	4	62	from	methylesterase	664:677	arg1	comparison					736:745	comparison	736:745	comparison to wild-type Col-0	736:764	In this work, we tested for the first time how cell wall dynamics affect photosynthetic properties in Arabidopsis thaliana pectin methylesterase atpme17.2 and pectin acetylesterase atpae11.1 mutants in comparison to wild-type Col-0.
32770751	4	63	dep	Arabidopsis	636:646	arg1	thaliana					648:655	thaliana	648:655	thaliana	648:655	In this work, we tested for the first time how cell wall dynamics affect photosynthetic properties in Arabidopsis thaliana pectin methylesterase atpme17.2 and pectin acetylesterase atpae11.1 mutants in comparison to wild-type Col-0.
32770751	5	64	theme	wall	899:902	arg1	sugars					927:932	cell wall non-cellulosic neutral sugars	894:932	cell wall non-cellulosic neutral sugars contents	894:941	Our results showed maintained PRE activities comparing mutants with wild-type and no significant differences in cellulose, but cell wall non-cellulosic neutral sugars contents changed.
32770751	4	65	theme	wall	586:589	arg1	dynamics					591:598	cell wall dynamics	581:598	cell wall dynamics	581:598	In this work, we tested for the first time how cell wall dynamics affect photosynthetic properties in Arabidopsis thaliana pectin methylesterase atpme17.2 and pectin acetylesterase atpae11.1 mutants in comparison to wild-type Col-0.
32770751	3	66	theme	due	410:412	arg1	Changes					364:370	Changes	364:370	Changes	364:370	Changes in pectin physicochemical properties, due to pectin remodeling enzymes (PRE), induce the rearrangement of cell wall compounds, thus, modifying wall architecture.
32770751	7	67	theme	decreased	1184:1192	arg1	assimilation					1202:1213	a decreased net CO2 assimilation	1182:1213	a decreased net CO2 assimilation (AN )	1182:1219	Additionally, physiological characterization revealed that mutants presented a decreased net CO2 assimilation (AN ) because of reductions in both stomatal (gs ) and mesophyll conductances (gm ).
32770751	5	68	theme	non-cellulosic	904:917	arg1	sugars					927:932	cell wall non-cellulosic neutral sugars	894:932	cell wall non-cellulosic neutral sugars contents	894:941	Our results showed maintained PRE activities comparing mutants with wild-type and no significant differences in cellulose, but cell wall non-cellulosic neutral sugars contents changed.
32770751	4	69	theme	cell	581:584	arg1	dynamics					591:598	cell wall dynamics	581:598	cell wall dynamics	581:598	In this work, we tested for the first time how cell wall dynamics affect photosynthetic properties in Arabidopsis thaliana pectin methylesterase atpme17.2 and pectin acetylesterase atpae11.1 mutants in comparison to wild-type Col-0.
33407409	6	0	theme	metabolome	739:748	arg1	analysis					750:757	the metabolome analysis	735:757	the metabolome analysis	735:757	In addition, the metabolome analysis revealed that treatment with oligofructose decreased 84 metabolites while 53 metabolites increased, such as dihydrothymine, N3,N4-Dimethyl-L-arginine, 10E,12Z-Octadecadienoic acid, and asparagine.
33407409	4	1	with	treatment	439:447	arg1	oligofructose					454:466	oligofructose	454:466	oligofructose	454:466	RESULTS Animals submitted to treatment with oligofructose had lower fecal pH but higher lactic acid, histamine, and Lipopolysaccharide (LPS) in serum.
33407409	9	2	theme	microbiota	1554:1563	arg1	disturbance					1535:1545	disturbance	1535:1545	disturbance of gut microbiota and changes of metabolites	1535:1590	CONCLUSIONS These results revealed that disturbance of gut microbiota and changes of metabolites were occurred during the development of equine laminitis, and these results may provide novel insights to detect biomarkers for a better understanding of the potential mechanism and prevention strategies for laminitis in horses.
33407409	0	3	theme	oligofructose-induced	65:85	arg1	laminitis					87:95	oligofructose-induced laminitis	65:95	oligofructose-induced laminitis	65:95	Changes of microbial and metabolome of the equine hindgut during oligofructose-induced laminitis.
33407409	5	4	theme	relative	669:676	arg1	abundance					678:686	relative abundance	669:686	relative abundance of Lactobacillus and Megasphaera	669:719	Meanwhile, oligofructose altered composition of the hindgut bacterial community, demonstrated by increasing relative abundance of Lactobacillus and Megasphaera.
33407409	4	5	theme	RESULTS	410:416	arg1	Animals					418:424	RESULTS Animals	410:424	RESULTS Animals submitted to treatment with oligofructose	410:466	RESULTS Animals submitted to treatment with oligofructose had lower fecal pH but higher lactic acid, histamine, and Lipopolysaccharide (LPS) in serum.
33407409	8	6	theme	10E,12Z-Octadecadienoic	1449:1471	arg1	acid					1473:1476	10E,12Z-Octadecadienoic acid	1449:1476	10E,12Z-Octadecadienoic acid	1449:1476	Furthermore, correlation analysis between gut microbiota and metabolites indicated that Lactobacillus and/or Megasphaera were positively associated with the dihydrothymine, N3,N4-Dimethyl-L-arginine, 10E,12Z-Octadecadienoic acid, and asparagine.
33407409	6	7	theme	10E,12Z-Octadecadienoic	910:932	arg1	acid					934:937	10E,12Z-Octadecadienoic acid	910:937	10E,12Z-Octadecadienoic acid	910:937	In addition, the metabolome analysis revealed that treatment with oligofructose decreased 84 metabolites while 53 metabolites increased, such as dihydrothymine, N3,N4-Dimethyl-L-arginine, 10E,12Z-Octadecadienoic acid, and asparagine.
33407409	9	8	theme	changes	1569:1575	arg1	disturbance					1535:1545	disturbance	1535:1545	disturbance of gut microbiota and changes of metabolites	1535:1590	CONCLUSIONS These results revealed that disturbance of gut microbiota and changes of metabolites were occurred during the development of equine laminitis, and these results may provide novel insights to detect biomarkers for a better understanding of the potential mechanism and prevention strategies for laminitis in horses.
33407409	4	9	theme	higher	491:496	arg1	acid					505:508	higher lactic acid	491:508	higher lactic acid	491:508	RESULTS Animals submitted to treatment with oligofructose had lower fecal pH but higher lactic acid, histamine, and Lipopolysaccharide (LPS) in serum.
33407409	5	10	theme	hindgut	613:619	arg1	community					631:639	the hindgut bacterial community	609:639	the hindgut bacterial community	609:639	Meanwhile, oligofructose altered composition of the hindgut bacterial community, demonstrated by increasing relative abundance of Lactobacillus and Megasphaera.
33407409	7	11	from	regulation	1024:1033	arg1	adipocytes					1051:1060	adipocytes	1051:1060	adipocytes	1051:1060	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	8	12	theme	correlation	1262:1272	arg1	analysis					1274:1281	correlation analysis	1262:1281	correlation analysis between gut microbiota and metabolites	1262:1320	Furthermore, correlation analysis between gut microbiota and metabolites indicated that Lactobacillus and/or Megasphaera were positively associated with the dihydrothymine, N3,N4-Dimethyl-L-arginine, 10E,12Z-Octadecadienoic acid, and asparagine.
33407409	5	13	theme	community	631:639	arg1	composition					594:604	oligofructose altered composition	572:604	oligofructose altered composition of the hindgut bacterial community	572:639	Meanwhile, oligofructose altered composition of the hindgut bacterial community, demonstrated by increasing relative abundance of Lactobacillus and Megasphaera.
33407409	5	13	theme	community	631:639	arg1	Meanwhile					561:569	Meanwhile	561:569	Meanwhile	561:569	Meanwhile, oligofructose altered composition of the hindgut bacterial community, demonstrated by increasing relative abundance of Lactobacillus and Megasphaera.
33407409	5	14	theme	bacterial	621:629	arg1	community					631:639	the hindgut bacterial community	609:639	the hindgut bacterial community	609:639	Meanwhile, oligofructose altered composition of the hindgut bacterial community, demonstrated by increasing relative abundance of Lactobacillus and Megasphaera.
33407409	8	15	theme	gut	1291:1293	arg1	microbiota					1295:1304	gut microbiota	1291:1304	gut microbiota	1291:1304	Furthermore, correlation analysis between gut microbiota and metabolites indicated that Lactobacillus and/or Megasphaera were positively associated with the dihydrothymine, N3,N4-Dimethyl-L-arginine, 10E,12Z-Octadecadienoic acid, and asparagine.
33407409	7	16	theme	galactose	1161:1169	arg1	metabolism					1171:1180	galactose metabolism	1161:1180	galactose metabolism	1161:1180	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	7	17	theme	healthy	1219:1225	arg1	horses					1241:1246	healthy and laminitis horses	1219:1246	healthy and laminitis horses	1219:1246	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	9	18	dep	CONCLUSIONS	1495:1505	arg1	revealed					1521:1528	revealed	1521:1528	revealed that disturbance of gut microbiota and changes of metabolites were occurred during the development of equine laminitis	1521:1647	CONCLUSIONS These results revealed that disturbance of gut microbiota and changes of metabolites were occurred during the development of equine laminitis, and these results may provide novel insights to detect biomarkers for a better understanding of the potential mechanism and prevention strategies for laminitis in horses.
33407409	9	19	from	laminitis	1800:1808	arg1	horses					1813:1818	horses	1813:1818	horses	1813:1818	CONCLUSIONS These results revealed that disturbance of gut microbiota and changes of metabolites were occurred during the development of equine laminitis, and these results may provide novel insights to detect biomarkers for a better understanding of the potential mechanism and prevention strategies for laminitis in horses.
33407409	4	20	theme	lactic	498:503	arg1	acid					505:508	higher lactic acid	491:508	higher lactic acid	491:508	RESULTS Animals submitted to treatment with oligofructose had lower fecal pH but higher lactic acid, histamine, and Lipopolysaccharide (LPS) in serum.
33407409	5	21	theme	oligofructose	572:584	arg1	composition					594:604	oligofructose altered composition	572:604	oligofructose altered composition of the hindgut bacterial community	572:639	Meanwhile, oligofructose altered composition of the hindgut bacterial community, demonstrated by increasing relative abundance of Lactobacillus and Megasphaera.
33407409	5	21	theme	oligofructose	572:584	arg1	Meanwhile					561:569	Meanwhile	561:569	Meanwhile	561:569	Meanwhile, oligofructose altered composition of the hindgut bacterial community, demonstrated by increasing relative abundance of Lactobacillus and Megasphaera.
33407409	1	22	theme	pathological	182:193	arg1	changes					195:201	pathological changes	182:201	pathological changes	182:201	BACKGROUND Laminitis is a common and serve disease which caused by inflammation and pathological changes of the laminar junction.
33407409	3	23	theme	horses	402:407	arg1	laminitis					389:397	oligofructose-induced laminitis	367:397	oligofructose-induced laminitis of horses	367:407	In this study we aimed to investigate changes of the gut microbiota and metabolomics in oligofructose-induced laminitis of horses.
33407409	5	24	theme	altered	586:592	arg1	composition					594:604	oligofructose altered composition	572:604	oligofructose altered composition of the hindgut bacterial community	572:639	Meanwhile, oligofructose altered composition of the hindgut bacterial community, demonstrated by increasing relative abundance of Lactobacillus and Megasphaera.
33407409	5	24	theme	altered	586:592	arg1	Meanwhile					561:569	Meanwhile	561:569	Meanwhile	561:569	Meanwhile, oligofructose altered composition of the hindgut bacterial community, demonstrated by increasing relative abundance of Lactobacillus and Megasphaera.
33407409	1	25	theme	BACKGROUND	98:107	arg1	Laminitis					109:117	BACKGROUND Laminitis	98:117	BACKGROUND Laminitis	98:117	BACKGROUND Laminitis is a common and serve disease which caused by inflammation and pathological changes of the laminar junction.
33407409	1	25	theme	BACKGROUND	98:107	arg1	disease					141:147	a common and serve disease	122:147	a common and serve disease which caused by inflammation and pathological changes of the laminar junction	122:225	BACKGROUND Laminitis is a common and serve disease which caused by inflammation and pathological changes of the laminar junction.
33407409	9	26	theme	gut	1550:1552	arg1	microbiota					1554:1563	gut microbiota	1550:1563	gut microbiota	1550:1563	CONCLUSIONS These results revealed that disturbance of gut microbiota and changes of metabolites were occurred during the development of equine laminitis, and these results may provide novel insights to detect biomarkers for a better understanding of the potential mechanism and prevention strategies for laminitis in horses.
33407409	3	27	theme	oligofructose-induced	367:387	arg1	laminitis					389:397	oligofructose-induced laminitis	367:397	oligofructose-induced laminitis of horses	367:407	In this study we aimed to investigate changes of the gut microbiota and metabolomics in oligofructose-induced laminitis of horses.
33407409	7	28	theme	fatty	1144:1148	arg1	acids					1150:1154	unsaturated fatty acids	1132:1154	unsaturated fatty acids	1132:1154	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	7	29	theme	aldosterone	987:997	arg1	synthesis					999:1007	aldosterone synthesis	987:1007	aldosterone synthesis	987:1007	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	9	30	theme	better	1722:1727	arg1	understanding					1729:1741	a better understanding	1720:1741	a better understanding of the potential mechanism	1720:1768	CONCLUSIONS These results revealed that disturbance of gut microbiota and changes of metabolites were occurred during the development of equine laminitis, and these results may provide novel insights to detect biomarkers for a better understanding of the potential mechanism and prevention strategies for laminitis in horses.
33407409	9	31	theme	metabolites	1580:1590	arg1	changes					1569:1575	changes	1569:1575	changes of metabolites	1569:1590	CONCLUSIONS These results revealed that disturbance of gut microbiota and changes of metabolites were occurred during the development of equine laminitis, and these results may provide novel insights to detect biomarkers for a better understanding of the potential mechanism and prevention strategies for laminitis in horses.
33407409	9	31	theme	metabolites	1580:1590	arg1	microbiota					1554:1563	gut microbiota	1550:1563	gut microbiota	1550:1563	CONCLUSIONS These results revealed that disturbance of gut microbiota and changes of metabolites were occurred during the development of equine laminitis, and these results may provide novel insights to detect biomarkers for a better understanding of the potential mechanism and prevention strategies for laminitis in horses.
33407409	0	32	theme	microbial	11:19	arg1	Changes					0:6	Changes	0:6	Changes of microbial and metabolome of the equine hindgut during oligofructose-induced laminitis.	0:96	Changes of microbial and metabolome of the equine hindgut during oligofructose-induced laminitis.
33407409	7	33	theme	acids	1150:1154	arg1	metabolism					1104:1113	pyrimidine metabolism	1093:1113	pyrimidine metabolism	1093:1113	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	7	33	theme	acids	1150:1154	arg1	secretion					1013:1021	secretion	1013:1021	secretion	1013:1021	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	7	33	theme	acids	1150:1154	arg1	biosynthesis					1116:1127	biosynthesis	1116:1127	biosynthesis of unsaturated fatty acids	1116:1154	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	7	33	theme	acids	1150:1154	arg1	regulation					1024:1033	regulation	1024:1033	regulation of lipolysis in adipocytes	1024:1060	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	7	33	theme	acids	1150:1154	arg1	metabolism					1171:1180	galactose metabolism	1161:1180	galactose metabolism	1161:1180	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	7	33	theme	acids	1150:1154	arg1	biosynthesis					1079:1090	steroid hormone biosynthesis	1063:1090	steroid hormone biosynthesis	1063:1090	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	7	33	theme	acids	1150:1154	arg1	synthesis					999:1007	aldosterone synthesis	987:1007	aldosterone synthesis	987:1007	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	4	34	theme	lower	472:476	arg1	pH					484:485	lower fecal pH	472:485	lower fecal pH	472:485	RESULTS Animals submitted to treatment with oligofructose had lower fecal pH but higher lactic acid, histamine, and Lipopolysaccharide (LPS) in serum.
33407409	9	35	theme	novel	1680:1684	arg1	insights					1686:1693	novel insights	1680:1693	novel insights	1680:1693	CONCLUSIONS These results revealed that disturbance of gut microbiota and changes of metabolites were occurred during the development of equine laminitis, and these results may provide novel insights to detect biomarkers for a better understanding of the potential mechanism and prevention strategies for laminitis in horses.
33407409	7	36	theme	laminitis	1231:1239	arg1	horses					1241:1246	healthy and laminitis horses	1219:1246	healthy and laminitis horses	1219:1246	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	0	37	theme	hindgut	50:56	arg1	microbial					11:19	microbial	11:19	microbial	11:19	Changes of microbial and metabolome of the equine hindgut during oligofructose-induced laminitis.
33407409	1	38	theme	laminar	210:216	arg1	junction					218:225	the laminar junction	206:225	the laminar junction	206:225	BACKGROUND Laminitis is a common and serve disease which caused by inflammation and pathological changes of the laminar junction.
33407409	6	39	with	treatment	773:781	arg1	oligofructose					788:800	oligofructose	788:800	oligofructose	788:800	In addition, the metabolome analysis revealed that treatment with oligofructose decreased 84 metabolites while 53 metabolites increased, such as dihydrothymine, N3,N4-Dimethyl-L-arginine, 10E,12Z-Octadecadienoic acid, and asparagine.
33407409	4	40	contain	had	468:470	arg2	acid					505:508	higher lactic acid	491:508	higher lactic acid	491:508	RESULTS Animals submitted to treatment with oligofructose had lower fecal pH but higher lactic acid, histamine, and Lipopolysaccharide (LPS) in serum.
33407409	4	40	contain	had	468:470	arg2	pH					484:485	lower fecal pH	472:485	lower fecal pH	472:485	RESULTS Animals submitted to treatment with oligofructose had lower fecal pH but higher lactic acid, histamine, and Lipopolysaccharide (LPS) in serum.
33407409	4	40	contain	had	468:470	arg2	Lipopolysaccharide					526:543	Lipopolysaccharide	526:543	Lipopolysaccharide (LPS)	526:549	RESULTS Animals submitted to treatment with oligofructose had lower fecal pH but higher lactic acid, histamine, and Lipopolysaccharide (LPS) in serum.
33407409	4	40	contain	had	468:470	arg2	histamine					511:519	histamine	511:519	histamine	511:519	RESULTS Animals submitted to treatment with oligofructose had lower fecal pH but higher lactic acid, histamine, and Lipopolysaccharide (LPS) in serum.
33407409	4	40	contain	had	468:470	arg2	LPS					546:548	LPS	546:548	LPS	546:548	RESULTS Animals submitted to treatment with oligofructose had lower fecal pH but higher lactic acid, histamine, and Lipopolysaccharide (LPS) in serum.
33407409	4	40	contain	had	468:470	arg1	Animals					418:424	RESULTS Animals	410:424	RESULTS Animals submitted to treatment with oligofructose	410:466	RESULTS Animals submitted to treatment with oligofructose had lower fecal pH but higher lactic acid, histamine, and Lipopolysaccharide (LPS) in serum.
33407409	0	41	theme	metabolome	25:34	arg1	Changes					0:6	Changes	0:6	Changes of microbial and metabolome of the equine hindgut during oligofructose-induced laminitis.	0:96	Changes of microbial and metabolome of the equine hindgut during oligofructose-induced laminitis.
33407409	3	42	dep	gut	332:334	arg1	metabolomics					351:362	metabolomics	351:362	metabolomics	351:362	In this study we aimed to investigate changes of the gut microbiota and metabolomics in oligofructose-induced laminitis of horses.
33407409	3	42	dep	gut	332:334	arg1	microbiota					336:345	microbiota	336:345	microbiota	336:345	In this study we aimed to investigate changes of the gut microbiota and metabolomics in oligofructose-induced laminitis of horses.
33407409	1	43	theme	junction	218:225	arg1	inflammation					165:176	inflammation	165:176	inflammation	165:176	BACKGROUND Laminitis is a common and serve disease which caused by inflammation and pathological changes of the laminar junction.
33407409	1	43	theme	junction	218:225	arg1	changes					195:201	pathological changes	182:201	pathological changes	182:201	BACKGROUND Laminitis is a common and serve disease which caused by inflammation and pathological changes of the laminar junction.
33407409	0	44	theme	equine	43:48	arg1	hindgut					50:56	the equine hindgut	39:56	the equine hindgut	39:56	Changes of microbial and metabolome of the equine hindgut during oligofructose-induced laminitis.
33407409	5	45	theme	Lactobacillus	691:703	arg1	abundance					678:686	relative abundance	669:686	relative abundance of Lactobacillus and Megasphaera	669:719	Meanwhile, oligofructose altered composition of the hindgut bacterial community, demonstrated by increasing relative abundance of Lactobacillus and Megasphaera.
33407409	4	46	theme	fecal	478:482	arg1	pH					484:485	lower fecal pH	472:485	lower fecal pH	472:485	RESULTS Animals submitted to treatment with oligofructose had lower fecal pH but higher lactic acid, histamine, and Lipopolysaccharide (LPS) in serum.
33407409	9	47	theme	potential	1750:1758	arg1	mechanism					1760:1768	the potential mechanism	1746:1768	the potential mechanism	1746:1768	CONCLUSIONS These results revealed that disturbance of gut microbiota and changes of metabolites were occurred during the development of equine laminitis, and these results may provide novel insights to detect biomarkers for a better understanding of the potential mechanism and prevention strategies for laminitis in horses.
33407409	7	48	theme	pyrimidine	1093:1102	arg1	metabolism					1104:1113	pyrimidine metabolism	1093:1113	pyrimidine metabolism	1093:1113	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	9	49	theme	laminitis	1639:1647	arg1	development					1617:1627	the development	1613:1627	the development of equine laminitis	1613:1647	CONCLUSIONS These results revealed that disturbance of gut microbiota and changes of metabolites were occurred during the development of equine laminitis, and these results may provide novel insights to detect biomarkers for a better understanding of the potential mechanism and prevention strategies for laminitis in horses.
33407409	7	50	theme	unsaturated	1132:1142	arg1	acids					1150:1154	unsaturated fatty acids	1132:1154	unsaturated fatty acids	1132:1154	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	7	51	from	biosynthesis	1079:1090	arg1	adipocytes					1051:1060	adipocytes	1051:1060	adipocytes	1051:1060	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	3	52	from	changes	317:323	arg1	laminitis					389:397	oligofructose-induced laminitis	367:397	oligofructose-induced laminitis of horses	367:407	In this study we aimed to investigate changes of the gut microbiota and metabolomics in oligofructose-induced laminitis of horses.
33407409	9	53	theme	mechanism	1760:1768	arg1	strategies					1785:1794	prevention strategies	1774:1794	prevention strategies for laminitis in horses	1774:1818	CONCLUSIONS These results revealed that disturbance of gut microbiota and changes of metabolites were occurred during the development of equine laminitis, and these results may provide novel insights to detect biomarkers for a better understanding of the potential mechanism and prevention strategies for laminitis in horses.
33407409	9	53	theme	mechanism	1760:1768	arg1	understanding					1729:1741	a better understanding	1720:1741	a better understanding of the potential mechanism	1720:1768	CONCLUSIONS These results revealed that disturbance of gut microbiota and changes of metabolites were occurred during the development of equine laminitis, and these results may provide novel insights to detect biomarkers for a better understanding of the potential mechanism and prevention strategies for laminitis in horses.
33407409	7	54	theme	steroid	1063:1069	arg1	biosynthesis					1079:1090	steroid hormone biosynthesis	1063:1090	steroid hormone biosynthesis	1063:1090	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	1	55	theme	common	124:129	arg1	Laminitis					109:117	BACKGROUND Laminitis	98:117	BACKGROUND Laminitis	98:117	BACKGROUND Laminitis is a common and serve disease which caused by inflammation and pathological changes of the laminar junction.
33407409	1	55	theme	common	124:129	arg1	disease					141:147	a common and serve disease	122:147	a common and serve disease which caused by inflammation and pathological changes of the laminar junction	122:225	BACKGROUND Laminitis is a common and serve disease which caused by inflammation and pathological changes of the laminar junction.
33407409	8	56	dep	dihydrothymine	1406:1419	arg1	the					1402:1404	the	1402:1404	the	1402:1404	Furthermore, correlation analysis between gut microbiota and metabolites indicated that Lactobacillus and/or Megasphaera were positively associated with the dihydrothymine, N3,N4-Dimethyl-L-arginine, 10E,12Z-Octadecadienoic acid, and asparagine.
33407409	5	57	theme	Megasphaera	709:719	arg1	abundance					678:686	relative abundance	669:686	relative abundance of Lactobacillus and Megasphaera	669:719	Meanwhile, oligofructose altered composition of the hindgut bacterial community, demonstrated by increasing relative abundance of Lactobacillus and Megasphaera.
33407409	7	58	theme	hormone	1071:1077	arg1	biosynthesis					1079:1090	steroid hormone biosynthesis	1063:1090	steroid hormone biosynthesis	1063:1090	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	7	59	from	synthesis	999:1007	arg1	adipocytes					1051:1060	adipocytes	1051:1060	adipocytes	1051:1060	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	9	60	theme	prevention	1774:1783	arg1	strategies					1785:1794	prevention strategies	1774:1794	prevention strategies for laminitis in horses	1774:1818	CONCLUSIONS These results revealed that disturbance of gut microbiota and changes of metabolites were occurred during the development of equine laminitis, and these results may provide novel insights to detect biomarkers for a better understanding of the potential mechanism and prevention strategies for laminitis in horses.
33407409	7	61	from	metabolism	1171:1180	arg1	adipocytes					1051:1060	adipocytes	1051:1060	adipocytes	1051:1060	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	1	62	theme	serve	135:139	arg1	Laminitis					109:117	BACKGROUND Laminitis	98:117	BACKGROUND Laminitis	98:117	BACKGROUND Laminitis is a common and serve disease which caused by inflammation and pathological changes of the laminar junction.
33407409	1	62	theme	serve	135:139	arg1	disease					141:147	a common and serve disease	122:147	a common and serve disease which caused by inflammation and pathological changes of the laminar junction	122:225	BACKGROUND Laminitis is a common and serve disease which caused by inflammation and pathological changes of the laminar junction.
33407409	7	63	from	metabolism	1104:1113	arg1	adipocytes					1051:1060	adipocytes	1051:1060	adipocytes	1051:1060	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	9	64	theme	equine	1632:1637	arg1	laminitis					1639:1647	equine laminitis	1632:1647	equine laminitis	1632:1647	CONCLUSIONS These results revealed that disturbance of gut microbiota and changes of metabolites were occurred during the development of equine laminitis, and these results may provide novel insights to detect biomarkers for a better understanding of the potential mechanism and prevention strategies for laminitis in horses.
33407409	7	65	from	secretion	1013:1021	arg1	adipocytes					1051:1060	adipocytes	1051:1060	adipocytes	1051:1060	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	7	66	theme	Pathway	956:962	arg1	analysis					964:971	Pathway analysis	956:971	Pathway analysis	956:971	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	3	67	theme	gut	332:334	arg1	changes					317:323	changes	317:323	changes of the gut microbiota and metabolomics in oligofructose-induced laminitis of horses	317:407	In this study we aimed to investigate changes of the gut microbiota and metabolomics in oligofructose-induced laminitis of horses.
33407409	2	68	theme	pathologic	241:250	arg1	mechanism					252:260	the pathologic mechanism	237:260	the pathologic mechanism	237:260	However, the pathologic mechanism remains unclear.
33407409	7	69	theme	lipolysis	1038:1046	arg1	metabolism					1104:1113	pyrimidine metabolism	1093:1113	pyrimidine metabolism	1093:1113	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	7	69	theme	lipolysis	1038:1046	arg1	secretion					1013:1021	secretion	1013:1021	secretion	1013:1021	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	7	69	theme	lipolysis	1038:1046	arg1	biosynthesis					1116:1127	biosynthesis	1116:1127	biosynthesis of unsaturated fatty acids	1116:1154	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	7	69	theme	lipolysis	1038:1046	arg1	regulation					1024:1033	regulation	1024:1033	regulation of lipolysis in adipocytes	1024:1060	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	7	69	theme	lipolysis	1038:1046	arg1	metabolism					1171:1180	galactose metabolism	1161:1180	galactose metabolism	1161:1180	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	7	69	theme	lipolysis	1038:1046	arg1	biosynthesis					1079:1090	steroid hormone biosynthesis	1063:1090	steroid hormone biosynthesis	1063:1090	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	7	69	theme	lipolysis	1038:1046	arg1	synthesis					999:1007	aldosterone synthesis	987:1007	aldosterone synthesis	987:1007	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
33407409	7	70	from	biosynthesis	1116:1127	arg1	adipocytes					1051:1060	adipocytes	1051:1060	adipocytes	1051:1060	Pathway analysis revealed that aldosterone synthesis and secretion, regulation of lipolysis in adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis of unsaturated fatty acids, and galactose metabolism were significantly different between healthy and laminitis horses.
34959178	0	0	theme	anti-inflammatory	74:90	arg1	activity					92:99	their anti-inflammatory activity	68:99	their anti-inflammatory activity	68:99	New aniline derivatives from the volva of Phallus rubrovolvatus and their anti-inflammatory activity.
34959178	5	1	theme	relative	683:690	arg1	configurations					705:718	the relative and absolute configurations	679:718	the relative and absolute configurations	679:718	Their structures were determined using 1D and 2D NMR data and HR-ESI-MS data, while the relative and absolute configurations were confirmed by NOESY correlations and comparison between experimental and calculated ECD spectra.
34959178	2	2	from	difficulty	297:306	arg1	processing					311:320	processing	311:320	processing	311:320	However, the volva of P. rubrovolvatus usually discarded as a by-product due to the unpleasant flavor and difficulty in processing.
34959178	6	3	theme	RAW264.7	950:957	arg1	macrophages					959:969	RAW264.7 macrophages	950:969	RAW264.7 macrophages	950:969	In addition, compounds 1-15 were tested for anti-inflammatory activity against lipopolysaccharide (LPS)-induced NO production in RAW264.7 macrophages.
34959178	1	4	from	species	173:179	arg1	China					184:188	China	184:188	China	184:188	Phallus rubrovolvatus is an important commercially cultivated mushroom species in China.
34959178	1	5	dep	important	130:138	arg1	cultivated					153:162	cultivated	153:162	cultivated	153:162	Phallus rubrovolvatus is an important commercially cultivated mushroom species in China.
34959178	3	6	theme	chemical	358:365	arg1	constituents					367:378	the chemical constituents	354:378	the chemical constituents	354:378	In this study, we investigated the chemical constituents and bioactivities of the volva of P. rubrovolvatus.
34959178	6	7	theme	NO	933:934	arg1	production					936:945	lipopolysaccharide (LPS)-induced NO production	900:945	lipopolysaccharide (LPS)-induced NO production in RAW264.7 macrophages	900:969	In addition, compounds 1-15 were tested for anti-inflammatory activity against lipopolysaccharide (LPS)-induced NO production in RAW264.7 macrophages.
34959178	4	8	theme	aniline	458:464	arg1	products					543:550	three new natural products	525:550	three new natural products (12, 13, 15)	525:563	As a result, fifteen rare aniline derivatives, including twelve new compounds (1-11, 14) and three new natural products (12, 13, 15) were isolated from the volva.
34959178	4	8	theme	aniline	458:464	arg1	compounds					500:508	twelve new compounds	489:508	twelve new compounds (1-11, 14)	489:519	As a result, fifteen rare aniline derivatives, including twelve new compounds (1-11, 14) and three new natural products (12, 13, 15) were isolated from the volva.
34959178	4	8	theme	aniline	458:464	arg1	derivatives					466:476	fifteen rare aniline derivatives	445:476	fifteen rare aniline derivatives	445:476	As a result, fifteen rare aniline derivatives, including twelve new compounds (1-11, 14) and three new natural products (12, 13, 15) were isolated from the volva.
34959178	4	8	theme	aniline	458:464	arg1	result					437:442	a result	435:442	a result	435:442	As a result, fifteen rare aniline derivatives, including twelve new compounds (1-11, 14) and three new natural products (12, 13, 15) were isolated from the volva.
34959178	6	9	from	production	936:945	arg1	macrophages					959:969	RAW264.7 macrophages	950:969	RAW264.7 macrophages	950:969	In addition, compounds 1-15 were tested for anti-inflammatory activity against lipopolysaccharide (LPS)-induced NO production in RAW264.7 macrophages.
34959178	2	10	theme	unpleasant	275:284	arg1	flavor					286:291	the unpleasant flavor	271:291	the unpleasant flavor	271:291	However, the volva of P. rubrovolvatus usually discarded as a by-product due to the unpleasant flavor and difficulty in processing.
34959178	5	11	theme	calculated	797:806	arg1	spectra					812:818	calculated ECD spectra	797:818	calculated ECD spectra	797:818	Their structures were determined using 1D and 2D NMR data and HR-ESI-MS data, while the relative and absolute configurations were confirmed by NOESY correlations and comparison between experimental and calculated ECD spectra.
34959178	0	12	from	volva	33:37	arg1	derivatives					12:22	New aniline derivatives	0:22	New aniline derivatives from the volva of Phallus rubrovolvatus	0:62	New aniline derivatives from the volva of Phallus rubrovolvatus and their anti-inflammatory activity.
34959178	0	12	from	volva	33:37	arg1	activity					92:99	their anti-inflammatory activity	68:99	their anti-inflammatory activity	68:99	New aniline derivatives from the volva of Phallus rubrovolvatus and their anti-inflammatory activity.
34959178	5	13	theme	NOESY	738:742	arg1	correlations					744:755	NOESY correlations	738:755	NOESY correlations	738:755	Their structures were determined using 1D and 2D NMR data and HR-ESI-MS data, while the relative and absolute configurations were confirmed by NOESY correlations and comparison between experimental and calculated ECD spectra.
34959178	5	14	theme	ECD	808:810	arg1	spectra					812:818	calculated ECD spectra	797:818	calculated ECD spectra	797:818	Their structures were determined using 1D and 2D NMR data and HR-ESI-MS data, while the relative and absolute configurations were confirmed by NOESY correlations and comparison between experimental and calculated ECD spectra.
34959178	0	15	theme	aniline	4:10	arg1	derivatives					12:22	New aniline derivatives	0:22	New aniline derivatives from the volva of Phallus rubrovolvatus	0:62	New aniline derivatives from the volva of Phallus rubrovolvatus and their anti-inflammatory activity.
34959178	7	16	theme	IC50	1036:1039	arg1	values					1041:1046	IC50 values	1036:1046	IC50 values ranging from 12.5 to 15.6 μM	1036:1075	Compounds 4, 9 and 10 exhibited anti-inflammatory activity with IC50 values ranging from 12.5 to 15.6 μM.
34959178	3	17	theme	rubrovolvatus	417:429	arg1	volva					405:409	the volva	401:409	the volva of P. rubrovolvatus	401:429	In this study, we investigated the chemical constituents and bioactivities of the volva of P. rubrovolvatus.
34959178	0	18	theme	New	0:2	arg1	derivatives					12:22	New aniline derivatives	0:22	New aniline derivatives from the volva of Phallus rubrovolvatus	0:62	New aniline derivatives from the volva of Phallus rubrovolvatus and their anti-inflammatory activity.
34959178	6	19	theme	-induced	924:931	arg1	production					936:945	lipopolysaccharide (LPS)-induced NO production	900:945	lipopolysaccharide (LPS)-induced NO production in RAW264.7 macrophages	900:969	In addition, compounds 1-15 were tested for anti-inflammatory activity against lipopolysaccharide (LPS)-induced NO production in RAW264.7 macrophages.
34959178	7	20	dep	15.6 μM	1069:1075	arg1	to					1066:1067	to	1066:1067	to	1066:1067	Compounds 4, 9 and 10 exhibited anti-inflammatory activity with IC50 values ranging from 12.5 to 15.6 μM.
34959178	1	21	theme	Phallus	102:108	arg1	species					173:179	an important commercially cultivated mushroom species	127:179	an important commercially cultivated mushroom species in China	127:188	Phallus rubrovolvatus is an important commercially cultivated mushroom species in China.
34959178	1	21	theme	Phallus	102:108	arg1	rubrovolvatus					110:122	Phallus rubrovolvatus	102:122	Phallus rubrovolvatus	102:122	Phallus rubrovolvatus is an important commercially cultivated mushroom species in China.
34959178	4	22	theme	natural	535:541	arg1	products					543:550	three new natural products	525:550	three new natural products (12, 13, 15)	525:563	As a result, fifteen rare aniline derivatives, including twelve new compounds (1-11, 14) and three new natural products (12, 13, 15) were isolated from the volva.
34959178	5	23	theme	2D	641:642	arg1	data					648:651	1D and 2D NMR data	634:651	1D and 2D NMR data	634:651	Their structures were determined using 1D and 2D NMR data and HR-ESI-MS data, while the relative and absolute configurations were confirmed by NOESY correlations and comparison between experimental and calculated ECD spectra.
34959178	5	24	theme	absolute	696:703	arg1	configurations					705:718	the relative and absolute configurations	679:718	the relative and absolute configurations	679:718	Their structures were determined using 1D and 2D NMR data and HR-ESI-MS data, while the relative and absolute configurations were confirmed by NOESY correlations and comparison between experimental and calculated ECD spectra.
34959178	4	25	theme	new	531:533	arg1	products					543:550	three new natural products	525:550	three new natural products (12, 13, 15)	525:563	As a result, fifteen rare aniline derivatives, including twelve new compounds (1-11, 14) and three new natural products (12, 13, 15) were isolated from the volva.
34959178	5	26	theme	NMR	644:646	arg1	data					648:651	1D and 2D NMR data	634:651	1D and 2D NMR data	634:651	Their structures were determined using 1D and 2D NMR data and HR-ESI-MS data, while the relative and absolute configurations were confirmed by NOESY correlations and comparison between experimental and calculated ECD spectra.
34959178	7	27	theme	anti-inflammatory	1004:1020	arg1	activity					1022:1029	anti-inflammatory activity	1004:1029	anti-inflammatory activity	1004:1029	Compounds 4, 9 and 10 exhibited anti-inflammatory activity with IC50 values ranging from 12.5 to 15.6 μM.
34959178	4	28	theme	rare	453:456	arg1	products					543:550	three new natural products	525:550	three new natural products (12, 13, 15)	525:563	As a result, fifteen rare aniline derivatives, including twelve new compounds (1-11, 14) and three new natural products (12, 13, 15) were isolated from the volva.
34959178	4	28	theme	rare	453:456	arg1	compounds					500:508	twelve new compounds	489:508	twelve new compounds (1-11, 14)	489:519	As a result, fifteen rare aniline derivatives, including twelve new compounds (1-11, 14) and three new natural products (12, 13, 15) were isolated from the volva.
34959178	4	28	theme	rare	453:456	arg1	derivatives					466:476	fifteen rare aniline derivatives	445:476	fifteen rare aniline derivatives	445:476	As a result, fifteen rare aniline derivatives, including twelve new compounds (1-11, 14) and three new natural products (12, 13, 15) were isolated from the volva.
34959178	4	28	theme	rare	453:456	arg1	result					437:442	a result	435:442	a result	435:442	As a result, fifteen rare aniline derivatives, including twelve new compounds (1-11, 14) and three new natural products (12, 13, 15) were isolated from the volva.
34959178	0	29	theme	Phallus	42:48	arg1	rubrovolvatus					50:62	Phallus rubrovolvatus	42:62	Phallus rubrovolvatus	42:62	New aniline derivatives from the volva of Phallus rubrovolvatus and their anti-inflammatory activity.
34959178	4	30	dep	compounds	500:508	arg1	1-11					511:514	1-11	511:514	1-11	511:514	As a result, fifteen rare aniline derivatives, including twelve new compounds (1-11, 14) and three new natural products (12, 13, 15) were isolated from the volva.
34959178	4	31	dep	products	543:550	arg1	15					561:562	15	561:562	15	561:562	As a result, fifteen rare aniline derivatives, including twelve new compounds (1-11, 14) and three new natural products (12, 13, 15) were isolated from the volva.
34959178	3	32	theme	volva	405:409	arg1	bioactivities					384:396	bioactivities	384:396	bioactivities	384:396	In this study, we investigated the chemical constituents and bioactivities of the volva of P. rubrovolvatus.
34959178	3	32	theme	volva	405:409	arg1	constituents					367:378	the chemical constituents	354:378	the chemical constituents	354:378	In this study, we investigated the chemical constituents and bioactivities of the volva of P. rubrovolvatus.
34959178	2	33	theme	rubrovolvatus	216:228	arg1	volva					204:208	the volva	200:208	the volva of P. rubrovolvatus	200:228	However, the volva of P. rubrovolvatus usually discarded as a by-product due to the unpleasant flavor and difficulty in processing.
34959178	6	34	theme	anti-inflammatory	865:881	arg1	activity					883:890	anti-inflammatory activity	865:890	anti-inflammatory activity against lipopolysaccharide (LPS)-induced NO production in RAW264.7 macrophages	865:969	In addition, compounds 1-15 were tested for anti-inflammatory activity against lipopolysaccharide (LPS)-induced NO production in RAW264.7 macrophages.
34959178	4	35	attach	isolated	570:577	arg2	products					543:550	three new natural products	525:550	three new natural products (12, 13, 15)	525:563	As a result, fifteen rare aniline derivatives, including twelve new compounds (1-11, 14) and three new natural products (12, 13, 15) were isolated from the volva.
34959178	4	35	attach	isolated	570:577	arg2	compounds					500:508	twelve new compounds	489:508	twelve new compounds (1-11, 14)	489:519	As a result, fifteen rare aniline derivatives, including twelve new compounds (1-11, 14) and three new natural products (12, 13, 15) were isolated from the volva.
34959178	4	35	attach	isolated	570:577	arg1	volva					588:592	the volva	584:592	the volva	584:592	As a result, fifteen rare aniline derivatives, including twelve new compounds (1-11, 14) and three new natural products (12, 13, 15) were isolated from the volva.
34959178	4	35	attach	isolated	570:577	arg2	derivatives					466:476	fifteen rare aniline derivatives	445:476	fifteen rare aniline derivatives	445:476	As a result, fifteen rare aniline derivatives, including twelve new compounds (1-11, 14) and three new natural products (12, 13, 15) were isolated from the volva.
34959178	4	35	attach	isolated	570:577	arg2	result					437:442	a result	435:442	a result	435:442	As a result, fifteen rare aniline derivatives, including twelve new compounds (1-11, 14) and three new natural products (12, 13, 15) were isolated from the volva.
34959178	1	36	theme	important	130:138	arg1	species					173:179	an important commercially cultivated mushroom species	127:179	an important commercially cultivated mushroom species in China	127:188	Phallus rubrovolvatus is an important commercially cultivated mushroom species in China.
34959178	1	36	theme	important	130:138	arg1	rubrovolvatus					110:122	Phallus rubrovolvatus	102:122	Phallus rubrovolvatus	102:122	Phallus rubrovolvatus is an important commercially cultivated mushroom species in China.
34959178	5	37	theme	1D	634:635	arg1	data					648:651	1D and 2D NMR data	634:651	1D and 2D NMR data	634:651	Their structures were determined using 1D and 2D NMR data and HR-ESI-MS data, while the relative and absolute configurations were confirmed by NOESY correlations and comparison between experimental and calculated ECD spectra.
34959178	2	38	from	flavor	286:291	arg1	processing					311:320	processing	311:320	processing	311:320	However, the volva of P. rubrovolvatus usually discarded as a by-product due to the unpleasant flavor and difficulty in processing.
34959178	0	39	theme	rubrovolvatus	50:62	arg1	volva					33:37	the volva	29:37	the volva of Phallus rubrovolvatus	29:62	New aniline derivatives from the volva of Phallus rubrovolvatus and their anti-inflammatory activity.
34959178	4	40	theme	new	496:498	arg1	compounds					500:508	twelve new compounds	489:508	twelve new compounds (1-11, 14)	489:519	As a result, fifteen rare aniline derivatives, including twelve new compounds (1-11, 14) and three new natural products (12, 13, 15) were isolated from the volva.
34959178	1	41	theme	mushroom	164:171	arg1	species					173:179	an important commercially cultivated mushroom species	127:179	an important commercially cultivated mushroom species in China	127:188	Phallus rubrovolvatus is an important commercially cultivated mushroom species in China.
34959178	1	41	theme	mushroom	164:171	arg1	rubrovolvatus					110:122	Phallus rubrovolvatus	102:122	Phallus rubrovolvatus	102:122	Phallus rubrovolvatus is an important commercially cultivated mushroom species in China.
34959178	5	42	theme	HR-ESI-MS	657:665	arg1	data					667:670	HR-ESI-MS data	657:670	HR-ESI-MS data	657:670	Their structures were determined using 1D and 2D NMR data and HR-ESI-MS data, while the relative and absolute configurations were confirmed by NOESY correlations and comparison between experimental and calculated ECD spectra.
32224167	9	0	theme	Candida	1270:1276	arg1	albicans					1278:1285	Candida albicans	1270:1285	Candida albicans	1270:1285	The antimicrobial activity of mat against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans was proved.
32224167	9	1	theme	mat	1190:1192	arg1	activity					1178:1185	The antimicrobial activity	1160:1185	The antimicrobial activity of mat against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans	1160:1285	The antimicrobial activity of mat against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans was proved.
32224167	5	2	theme	ICP	847:849	arg1	analyses					851:858	UV-vis and ICP analyses	836:858	UV-vis and ICP analyses	836:858	The synthesis of AgNPs was supported by UV-vis and ICP analyses.
32224167	4	3	theme	GA-polyvinyl	553:564	arg1	mat					591:593	polycaprolacton (PCL)-coated GA-polyvinyl alcohol (PVA) nanofibers mat	524:593	polycaprolacton (PCL)-coated GA-polyvinyl alcohol (PVA) nanofibers mat	524:593	For this purpose, the composition of polycaprolacton (PCL)-coated GA-polyvinyl alcohol (PVA) nanofibers mat was optimized based on the possible highest porosity, water absorption and water vapor permeability, and then silver nanoparticles (AgNPs) loaded nanofibers mat was prepared based on this composition.
32224167	7	4	theme	%	1122:1122	arg1	250 nm					1033:1038	250 nm	1033:1038	250 nm	1033:1038	The results showed that the average diameter of nanofibers was in the range of 150 to 250 nm with the porosity, water absorption and water vapor permeability of 37.34%, 547.30% and 2235.50 g/m2.
32224167	7	4	theme	%	1122:1122	arg1	absorption					1065:1074	water absorption	1059:1074	water absorption	1059:1074	The results showed that the average diameter of nanofibers was in the range of 150 to 250 nm with the porosity, water absorption and water vapor permeability of 37.34%, 547.30% and 2235.50 g/m2.
32224167	7	4	theme	%	1122:1122	arg1	permeability					1092:1103	water vapor permeability	1080:1103	water vapor permeability	1080:1103	The results showed that the average diameter of nanofibers was in the range of 150 to 250 nm with the porosity, water absorption and water vapor permeability of 37.34%, 547.30% and 2235.50 g/m2.
32224167	3	5	from	GA	392:393	arg1	mat					383:385	an antimicrobial nanofibers mat	355:385	an antimicrobial nanofibers mat from GA with suitable porosity, water absorption, water vapor permeability and mechanical strength	355:484	The aim of this study was to fabricate an antimicrobial nanofibers mat from GA with suitable porosity, water absorption, water vapor permeability and mechanical strength.
32224167	11	6	theme	antimicrobial	1470:1482	arg1	GA-PVA-AgNPs					1441:1452	PCL-coated GA-PVA-AgNPs	1430:1452	PCL-coated GA-PVA-AgNPs	1430:1452	This study introduced PCL-coated GA-PVA-AgNPs as an effective antimicrobial mat alternative for commercial wound dressing.
32224167	11	6	theme	antimicrobial	1470:1482	arg1	alternative					1488:1498	an effective antimicrobial mat alternative	1457:1498	an effective antimicrobial mat alternative for commercial wound dressing	1457:1528	This study introduced PCL-coated GA-PVA-AgNPs as an effective antimicrobial mat alternative for commercial wound dressing.
32224167	4	7	theme	-coated	545:551	arg1	mat					591:593	polycaprolacton (PCL)-coated GA-polyvinyl alcohol (PVA) nanofibers mat	524:593	polycaprolacton (PCL)-coated GA-polyvinyl alcohol (PVA) nanofibers mat	524:593	For this purpose, the composition of polycaprolacton (PCL)-coated GA-polyvinyl alcohol (PVA) nanofibers mat was optimized based on the possible highest porosity, water absorption and water vapor permeability, and then silver nanoparticles (AgNPs) loaded nanofibers mat was prepared based on this composition.
32224167	0	8	theme	gum	109:111	arg1	nanofibers					120:129	loaded gum Arabic nanofibers	102:129	loaded gum Arabic nanofibers	102:129	Synthesis and characterization of antimicrobial wound dressing material based on silver nanoparticles loaded gum Arabic nanofibers.
32224167	4	9	theme	nanofibers	741:750	arg1	mat					752:754	nanofibers mat	741:754	nanofibers mat	741:754	For this purpose, the composition of polycaprolacton (PCL)-coated GA-polyvinyl alcohol (PVA) nanofibers mat was optimized based on the possible highest porosity, water absorption and water vapor permeability, and then silver nanoparticles (AgNPs) loaded nanofibers mat was prepared based on this composition.
32224167	10	10	theme	good	1344:1347	arg1	biocompatibility					1349:1364	the good biocompatibility	1340:1364	the good biocompatibility for the mouse embryonic fibroblast cells	1340:1405	Moreover, the cytotoxicity of mat showed the good biocompatibility for the mouse embryonic fibroblast cells.
32224167	7	11	dep	250 nm	1033:1038	arg1	to					1030:1031	to	1030:1031	to	1030:1031	The results showed that the average diameter of nanofibers was in the range of 150 to 250 nm with the porosity, water absorption and water vapor permeability of 37.34%, 547.30% and 2235.50 g/m2.
32224167	2	12	theme	due	276:278	arg1	bottleneck					265:274	a bottleneck	263:274	a bottleneck due to its physico-chemical properties	263:313	However, electrospinning of GA is a bottleneck due to its physico-chemical properties.
32224167	2	12	theme	due	276:278	arg1	electrospinning					238:252	electrospinning	238:252	electrospinning of GA	238:258	However, electrospinning of GA is a bottleneck due to its physico-chemical properties.
32224167	4	13	theme	possible	622:629	arg1	AgNPs					727:731	AgNPs	727:731	AgNPs	727:731	For this purpose, the composition of polycaprolacton (PCL)-coated GA-polyvinyl alcohol (PVA) nanofibers mat was optimized based on the possible highest porosity, water absorption and water vapor permeability, and then silver nanoparticles (AgNPs) loaded nanofibers mat was prepared based on this composition.
32224167	4	13	theme	possible	622:629	arg1	porosity					639:646	porosity	639:646	porosity	639:646	For this purpose, the composition of polycaprolacton (PCL)-coated GA-polyvinyl alcohol (PVA) nanofibers mat was optimized based on the possible highest porosity, water absorption and water vapor permeability, and then silver nanoparticles (AgNPs) loaded nanofibers mat was prepared based on this composition.
32224167	0	14	theme	loaded	102:107	arg1	nanofibers					120:129	loaded gum Arabic nanofibers	102:129	loaded gum Arabic nanofibers	102:129	Synthesis and characterization of antimicrobial wound dressing material based on silver nanoparticles loaded gum Arabic nanofibers.
32224167	7	15	theme	250 nm	1033:1038	arg1	range					1017:1021	the range	1013:1021	the range of 150 to 250 nm with the porosity, water absorption and water vapor permeability of 37.34%, 547.30% and 2235.50 g/m2	1013:1139	The results showed that the average diameter of nanofibers was in the range of 150 to 250 nm with the porosity, water absorption and water vapor permeability of 37.34%, 547.30% and 2235.50 g/m2.
32224167	4	16	theme	alcohol	566:572	arg1	mat					591:593	polycaprolacton (PCL)-coated GA-polyvinyl alcohol (PVA) nanofibers mat	524:593	polycaprolacton (PCL)-coated GA-polyvinyl alcohol (PVA) nanofibers mat	524:593	For this purpose, the composition of polycaprolacton (PCL)-coated GA-polyvinyl alcohol (PVA) nanofibers mat was optimized based on the possible highest porosity, water absorption and water vapor permeability, and then silver nanoparticles (AgNPs) loaded nanofibers mat was prepared based on this composition.
32224167	4	17	theme	vapor	676:680	arg1	permeability					682:693	water vapor permeability	670:693	water vapor permeability	670:693	For this purpose, the composition of polycaprolacton (PCL)-coated GA-polyvinyl alcohol (PVA) nanofibers mat was optimized based on the possible highest porosity, water absorption and water vapor permeability, and then silver nanoparticles (AgNPs) loaded nanofibers mat was prepared based on this composition.
32224167	10	18	theme	fibroblast	1390:1399	arg1	cells					1401:1405	the mouse embryonic fibroblast cells	1370:1405	the mouse embryonic fibroblast cells	1370:1405	Moreover, the cytotoxicity of mat showed the good biocompatibility for the mouse embryonic fibroblast cells.
32224167	11	19	theme	effective	1460:1468	arg1	GA-PVA-AgNPs					1441:1452	PCL-coated GA-PVA-AgNPs	1430:1452	PCL-coated GA-PVA-AgNPs	1430:1452	This study introduced PCL-coated GA-PVA-AgNPs as an effective antimicrobial mat alternative for commercial wound dressing.
32224167	11	19	theme	effective	1460:1468	arg1	alternative					1488:1498	an effective antimicrobial mat alternative	1457:1498	an effective antimicrobial mat alternative for commercial wound dressing	1457:1528	This study introduced PCL-coated GA-PVA-AgNPs as an effective antimicrobial mat alternative for commercial wound dressing.
32224167	1	20	theme	biocompatible	153:165	arg1	Arabic					136:141	Gum Arabic	132:141	Gum Arabic (GA)	132:146	Gum Arabic (GA) is a biocompatible polymer with the necessary requirements for a wound dressing.
32224167	1	20	theme	biocompatible	153:165	arg1	polymer					167:173	a biocompatible polymer	151:173	a biocompatible polymer with the necessary requirements for a wound dressing	151:226	Gum Arabic (GA) is a biocompatible polymer with the necessary requirements for a wound dressing.
32224167	0	21	theme	Arabic	113:118	arg1	nanofibers					120:129	loaded gum Arabic nanofibers	102:129	loaded gum Arabic nanofibers	102:129	Synthesis and characterization of antimicrobial wound dressing material based on silver nanoparticles loaded gum Arabic nanofibers.
32224167	7	22	theme	water	1059:1063	arg1	250 nm					1033:1038	250 nm	1033:1038	250 nm	1033:1038	The results showed that the average diameter of nanofibers was in the range of 150 to 250 nm with the porosity, water absorption and water vapor permeability of 37.34%, 547.30% and 2235.50 g/m2.
32224167	7	22	theme	water	1059:1063	arg1	absorption					1065:1074	water absorption	1059:1074	water absorption	1059:1074	The results showed that the average diameter of nanofibers was in the range of 150 to 250 nm with the porosity, water absorption and water vapor permeability of 37.34%, 547.30% and 2235.50 g/m2.
32224167	11	23	theme	commercial	1504:1513	arg1	dressing					1521:1528	commercial wound dressing	1504:1528	commercial wound dressing	1504:1528	This study introduced PCL-coated GA-PVA-AgNPs as an effective antimicrobial mat alternative for commercial wound dressing.
32224167	7	24	theme	%	1113:1113	arg1	250 nm					1033:1038	250 nm	1033:1038	250 nm	1033:1038	The results showed that the average diameter of nanofibers was in the range of 150 to 250 nm with the porosity, water absorption and water vapor permeability of 37.34%, 547.30% and 2235.50 g/m2.
32224167	7	24	theme	%	1113:1113	arg1	absorption					1065:1074	water absorption	1059:1074	water absorption	1059:1074	The results showed that the average diameter of nanofibers was in the range of 150 to 250 nm with the porosity, water absorption and water vapor permeability of 37.34%, 547.30% and 2235.50 g/m2.
32224167	7	24	theme	%	1113:1113	arg1	permeability					1092:1103	water vapor permeability	1080:1103	water vapor permeability	1080:1103	The results showed that the average diameter of nanofibers was in the range of 150 to 250 nm with the porosity, water absorption and water vapor permeability of 37.34%, 547.30% and 2235.50 g/m2.
32224167	3	25	theme	water	419:423	arg1	absorption					425:434	water absorption	419:434	water absorption	419:434	The aim of this study was to fabricate an antimicrobial nanofibers mat from GA with suitable porosity, water absorption, water vapor permeability and mechanical strength.
32224167	3	26	theme	antimicrobial	358:370	arg1	mat					383:385	an antimicrobial nanofibers mat	355:385	an antimicrobial nanofibers mat from GA with suitable porosity, water absorption, water vapor permeability and mechanical strength	355:484	The aim of this study was to fabricate an antimicrobial nanofibers mat from GA with suitable porosity, water absorption, water vapor permeability and mechanical strength.
32224167	10	27	theme	mouse	1374:1378	arg1	cells					1401:1405	the mouse embryonic fibroblast cells	1370:1405	the mouse embryonic fibroblast cells	1370:1405	Moreover, the cytotoxicity of mat showed the good biocompatibility for the mouse embryonic fibroblast cells.
32224167	10	28	theme	mat	1329:1331	arg1	cytotoxicity					1313:1324	the cytotoxicity	1309:1324	the cytotoxicity of mat	1309:1331	Moreover, the cytotoxicity of mat showed the good biocompatibility for the mouse embryonic fibroblast cells.
32224167	3	29	theme	suitable	400:407	arg1	porosity					409:416	suitable porosity	400:416	suitable porosity	400:416	The aim of this study was to fabricate an antimicrobial nanofibers mat from GA with suitable porosity, water absorption, water vapor permeability and mechanical strength.
32224167	3	30	theme	nanofibers	372:381	arg1	mat					383:385	an antimicrobial nanofibers mat	355:385	an antimicrobial nanofibers mat from GA with suitable porosity, water absorption, water vapor permeability and mechanical strength	355:484	The aim of this study was to fabricate an antimicrobial nanofibers mat from GA with suitable porosity, water absorption, water vapor permeability and mechanical strength.
32224167	5	31	theme	AgNPs	813:817	arg1	synthesis					800:808	The synthesis	796:808	The synthesis of AgNPs	796:817	The synthesis of AgNPs was supported by UV-vis and ICP analyses.
32224167	2	32	theme	physico-chemical	287:302	arg1	properties					304:313	its physico-chemical properties	283:313	its physico-chemical properties	283:313	However, electrospinning of GA is a bottleneck due to its physico-chemical properties.
32224167	4	33	theme	highest	631:637	arg1	AgNPs					727:731	AgNPs	727:731	AgNPs	727:731	For this purpose, the composition of polycaprolacton (PCL)-coated GA-polyvinyl alcohol (PVA) nanofibers mat was optimized based on the possible highest porosity, water absorption and water vapor permeability, and then silver nanoparticles (AgNPs) loaded nanofibers mat was prepared based on this composition.
32224167	4	33	theme	highest	631:637	arg1	porosity					639:646	porosity	639:646	porosity	639:646	For this purpose, the composition of polycaprolacton (PCL)-coated GA-polyvinyl alcohol (PVA) nanofibers mat was optimized based on the possible highest porosity, water absorption and water vapor permeability, and then silver nanoparticles (AgNPs) loaded nanofibers mat was prepared based on this composition.
32224167	3	34	theme	water	437:441	arg1	permeability					449:460	water vapor permeability	437:460	water vapor permeability	437:460	The aim of this study was to fabricate an antimicrobial nanofibers mat from GA with suitable porosity, water absorption, water vapor permeability and mechanical strength.
32224167	7	35	theme	water	1080:1084	arg1	250 nm					1033:1038	250 nm	1033:1038	250 nm	1033:1038	The results showed that the average diameter of nanofibers was in the range of 150 to 250 nm with the porosity, water absorption and water vapor permeability of 37.34%, 547.30% and 2235.50 g/m2.
32224167	7	35	theme	water	1080:1084	arg1	permeability					1092:1103	water vapor permeability	1080:1103	water vapor permeability	1080:1103	The results showed that the average diameter of nanofibers was in the range of 150 to 250 nm with the porosity, water absorption and water vapor permeability of 37.34%, 547.30% and 2235.50 g/m2.
32224167	4	36	theme	silver	705:710	arg1	nanoparticles					712:724	silver nanoparticles	705:724	silver nanoparticles	705:724	For this purpose, the composition of polycaprolacton (PCL)-coated GA-polyvinyl alcohol (PVA) nanofibers mat was optimized based on the possible highest porosity, water absorption and water vapor permeability, and then silver nanoparticles (AgNPs) loaded nanofibers mat was prepared based on this composition.
32224167	3	37	theme	vapor	443:447	arg1	permeability					449:460	water vapor permeability	437:460	water vapor permeability	437:460	The aim of this study was to fabricate an antimicrobial nanofibers mat from GA with suitable porosity, water absorption, water vapor permeability and mechanical strength.
32224167	0	38	theme	wound	48:52	arg1	material					63:70	antimicrobial wound dressing material	34:70	antimicrobial wound dressing material	34:70	Synthesis and characterization of antimicrobial wound dressing material based on silver nanoparticles loaded gum Arabic nanofibers.
32224167	4	39	theme	water	670:674	arg1	permeability					682:693	water vapor permeability	670:693	water vapor permeability	670:693	For this purpose, the composition of polycaprolacton (PCL)-coated GA-polyvinyl alcohol (PVA) nanofibers mat was optimized based on the possible highest porosity, water absorption and water vapor permeability, and then silver nanoparticles (AgNPs) loaded nanofibers mat was prepared based on this composition.
32224167	0	40	theme	antimicrobial	34:46	arg1	material					63:70	antimicrobial wound dressing material	34:70	antimicrobial wound dressing material	34:70	Synthesis and characterization of antimicrobial wound dressing material based on silver nanoparticles loaded gum Arabic nanofibers.
32224167	6	41	theme	constituents	890:901	arg1	structure					865:873	The structure	861:873	The structure of mat and its constituents	861:901	The structure of mat and its constituents were characterized by FE-SEM, XRD and FTIR.
32224167	7	42	theme	nanofibers	995:1004	arg1	diameter					983:990	the average diameter	971:990	the average diameter of nanofibers	971:1004	The results showed that the average diameter of nanofibers was in the range of 150 to 250 nm with the porosity, water absorption and water vapor permeability of 37.34%, 547.30% and 2235.50 g/m2.
32224167	3	43	theme	study	332:336	arg1	aim					320:322	The aim	316:322	The aim of this study	316:336	The aim of this study was to fabricate an antimicrobial nanofibers mat from GA with suitable porosity, water absorption, water vapor permeability and mechanical strength.
32224167	0	44	theme	material	63:70	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Synthesis and characterization of antimicrobial wound dressing material based on silver nanoparticles loaded gum Arabic nanofibers.
32224167	0	44	theme	material	63:70	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterization of antimicrobial wound dressing material based on silver nanoparticles loaded gum Arabic nanofibers.
32224167	5	45	theme	UV-vis	836:841	arg1	analyses					851:858	UV-vis and ICP analyses	836:858	UV-vis and ICP analyses	836:858	The synthesis of AgNPs was supported by UV-vis and ICP analyses.
32224167	7	46	theme	average	975:981	arg1	diameter					983:990	the average diameter	971:990	the average diameter of nanofibers	971:1004	The results showed that the average diameter of nanofibers was in the range of 150 to 250 nm with the porosity, water absorption and water vapor permeability of 37.34%, 547.30% and 2235.50 g/m2.
32224167	3	47	theme	mechanical	466:475	arg1	strength					477:484	mechanical strength	466:484	mechanical strength	466:484	The aim of this study was to fabricate an antimicrobial nanofibers mat from GA with suitable porosity, water absorption, water vapor permeability and mechanical strength.
32224167	0	48	theme	dressing	54:61	arg1	material					63:70	antimicrobial wound dressing material	34:70	antimicrobial wound dressing material	34:70	Synthesis and characterization of antimicrobial wound dressing material based on silver nanoparticles loaded gum Arabic nanofibers.
32224167	10	49	theme	embryonic	1380:1388	arg1	cells					1401:1405	the mouse embryonic fibroblast cells	1370:1405	the mouse embryonic fibroblast cells	1370:1405	Moreover, the cytotoxicity of mat showed the good biocompatibility for the mouse embryonic fibroblast cells.
32224167	1	50	theme	Gum	132:134	arg1	Arabic					136:141	Gum Arabic	132:141	Gum Arabic (GA)	132:146	Gum Arabic (GA) is a biocompatible polymer with the necessary requirements for a wound dressing.
32224167	1	50	theme	Gum	132:134	arg1	polymer					167:173	a biocompatible polymer	151:173	a biocompatible polymer with the necessary requirements for a wound dressing	151:226	Gum Arabic (GA) is a biocompatible polymer with the necessary requirements for a wound dressing.
32224167	1	50	theme	Gum	132:134	arg1	GA					144:145	GA	144:145	GA	144:145	Gum Arabic (GA) is a biocompatible polymer with the necessary requirements for a wound dressing.
32224167	11	51	theme	wound	1515:1519	arg1	dressing					1521:1528	commercial wound dressing	1504:1528	commercial wound dressing	1504:1528	This study introduced PCL-coated GA-PVA-AgNPs as an effective antimicrobial mat alternative for commercial wound dressing.
32224167	6	52	theme	mat	878:880	arg1	structure					865:873	The structure	861:873	The structure of mat and its constituents	861:901	The structure of mat and its constituents were characterized by FE-SEM, XRD and FTIR.
32224167	2	53	theme	GA	257:258	arg1	bottleneck					265:274	a bottleneck	263:274	a bottleneck due to its physico-chemical properties	263:313	However, electrospinning of GA is a bottleneck due to its physico-chemical properties.
32224167	2	53	theme	GA	257:258	arg1	electrospinning					238:252	electrospinning	238:252	electrospinning of GA	238:258	However, electrospinning of GA is a bottleneck due to its physico-chemical properties.
32224167	11	54	theme	mat	1484:1486	arg1	GA-PVA-AgNPs					1441:1452	PCL-coated GA-PVA-AgNPs	1430:1452	PCL-coated GA-PVA-AgNPs	1430:1452	This study introduced PCL-coated GA-PVA-AgNPs as an effective antimicrobial mat alternative for commercial wound dressing.
32224167	11	54	theme	mat	1484:1486	arg1	alternative					1488:1498	an effective antimicrobial mat alternative	1457:1498	an effective antimicrobial mat alternative for commercial wound dressing	1457:1528	This study introduced PCL-coated GA-PVA-AgNPs as an effective antimicrobial mat alternative for commercial wound dressing.
32224167	1	55	theme	wound	213:217	arg1	dressing					219:226	a wound dressing	211:226	a wound dressing	211:226	Gum Arabic (GA) is a biocompatible polymer with the necessary requirements for a wound dressing.
32224167	1	56	with	polymer	167:173	arg1	requirements					194:205	the necessary requirements	180:205	the necessary requirements for a wound dressing	180:226	Gum Arabic (GA) is a biocompatible polymer with the necessary requirements for a wound dressing.
32224167	9	57	theme	antimicrobial	1164:1176	arg1	activity					1178:1185	The antimicrobial activity	1160:1185	The antimicrobial activity of mat against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans	1160:1285	The antimicrobial activity of mat against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans was proved.
32224167	3	58	with	mat	383:385	arg1	permeability					449:460	water vapor permeability	437:460	water vapor permeability	437:460	The aim of this study was to fabricate an antimicrobial nanofibers mat from GA with suitable porosity, water absorption, water vapor permeability and mechanical strength.
32224167	3	58	with	mat	383:385	arg1	strength					477:484	mechanical strength	466:484	mechanical strength	466:484	The aim of this study was to fabricate an antimicrobial nanofibers mat from GA with suitable porosity, water absorption, water vapor permeability and mechanical strength.
32224167	3	58	with	mat	383:385	arg1	porosity					409:416	suitable porosity	400:416	suitable porosity	400:416	The aim of this study was to fabricate an antimicrobial nanofibers mat from GA with suitable porosity, water absorption, water vapor permeability and mechanical strength.
32224167	3	58	with	mat	383:385	arg1	absorption					425:434	water absorption	419:434	water absorption	419:434	The aim of this study was to fabricate an antimicrobial nanofibers mat from GA with suitable porosity, water absorption, water vapor permeability and mechanical strength.
32224167	4	59	theme	PVA	575:577	arg1	mat					591:593	polycaprolacton (PCL)-coated GA-polyvinyl alcohol (PVA) nanofibers mat	524:593	polycaprolacton (PCL)-coated GA-polyvinyl alcohol (PVA) nanofibers mat	524:593	For this purpose, the composition of polycaprolacton (PCL)-coated GA-polyvinyl alcohol (PVA) nanofibers mat was optimized based on the possible highest porosity, water absorption and water vapor permeability, and then silver nanoparticles (AgNPs) loaded nanofibers mat was prepared based on this composition.
32224167	11	60	theme	PCL-coated	1430:1439	arg1	GA-PVA-AgNPs					1441:1452	PCL-coated GA-PVA-AgNPs	1430:1452	PCL-coated GA-PVA-AgNPs	1430:1452	This study introduced PCL-coated GA-PVA-AgNPs as an effective antimicrobial mat alternative for commercial wound dressing.
32224167	11	60	theme	PCL-coated	1430:1439	arg1	alternative					1488:1498	an effective antimicrobial mat alternative	1457:1498	an effective antimicrobial mat alternative for commercial wound dressing	1457:1528	This study introduced PCL-coated GA-PVA-AgNPs as an effective antimicrobial mat alternative for commercial wound dressing.
32224167	1	61	theme	necessary	184:192	arg1	requirements					194:205	the necessary requirements	180:205	the necessary requirements for a wound dressing	180:226	Gum Arabic (GA) is a biocompatible polymer with the necessary requirements for a wound dressing.
32224167	4	62	theme	water	649:653	arg1	absorption					655:664	water absorption	649:664	water absorption	649:664	For this purpose, the composition of polycaprolacton (PCL)-coated GA-polyvinyl alcohol (PVA) nanofibers mat was optimized based on the possible highest porosity, water absorption and water vapor permeability, and then silver nanoparticles (AgNPs) loaded nanofibers mat was prepared based on this composition.
32224167	4	63	theme	mat	591:593	arg1	composition					509:519	the composition	505:519	the composition of polycaprolacton (PCL)-coated GA-polyvinyl alcohol (PVA) nanofibers mat	505:593	For this purpose, the composition of polycaprolacton (PCL)-coated GA-polyvinyl alcohol (PVA) nanofibers mat was optimized based on the possible highest porosity, water absorption and water vapor permeability, and then silver nanoparticles (AgNPs) loaded nanofibers mat was prepared based on this composition.
32224167	7	64	theme	2235.50 g/m2	1128:1139	arg1	250 nm					1033:1038	250 nm	1033:1038	250 nm	1033:1038	The results showed that the average diameter of nanofibers was in the range of 150 to 250 nm with the porosity, water absorption and water vapor permeability of 37.34%, 547.30% and 2235.50 g/m2.
32224167	7	64	theme	2235.50 g/m2	1128:1139	arg1	absorption					1065:1074	water absorption	1059:1074	water absorption	1059:1074	The results showed that the average diameter of nanofibers was in the range of 150 to 250 nm with the porosity, water absorption and water vapor permeability of 37.34%, 547.30% and 2235.50 g/m2.
32224167	7	64	theme	2235.50 g/m2	1128:1139	arg1	permeability					1092:1103	water vapor permeability	1080:1103	water vapor permeability	1080:1103	The results showed that the average diameter of nanofibers was in the range of 150 to 250 nm with the porosity, water absorption and water vapor permeability of 37.34%, 547.30% and 2235.50 g/m2.
32224167	7	65	theme	vapor	1086:1090	arg1	250 nm					1033:1038	250 nm	1033:1038	250 nm	1033:1038	The results showed that the average diameter of nanofibers was in the range of 150 to 250 nm with the porosity, water absorption and water vapor permeability of 37.34%, 547.30% and 2235.50 g/m2.
32224167	7	65	theme	vapor	1086:1090	arg1	permeability					1092:1103	water vapor permeability	1080:1103	water vapor permeability	1080:1103	The results showed that the average diameter of nanofibers was in the range of 150 to 250 nm with the porosity, water absorption and water vapor permeability of 37.34%, 547.30% and 2235.50 g/m2.
32224167	4	66	theme	nanofibers	580:589	arg1	mat					591:593	polycaprolacton (PCL)-coated GA-polyvinyl alcohol (PVA) nanofibers mat	524:593	polycaprolacton (PCL)-coated GA-polyvinyl alcohol (PVA) nanofibers mat	524:593	For this purpose, the composition of polycaprolacton (PCL)-coated GA-polyvinyl alcohol (PVA) nanofibers mat was optimized based on the possible highest porosity, water absorption and water vapor permeability, and then silver nanoparticles (AgNPs) loaded nanofibers mat was prepared based on this composition.
35116628	1	0	gly	glycoprotein	149:160	arg1	glycoprotein					149:160	a membrane glycoprotein	138:160	a membrane glycoprotein mediating cell-to-cell signaling	138:193	BACKGROUND Neuregulin 1 (NRG1) is a membrane glycoprotein mediating cell-to-cell signaling and has a crucial role in the growth and development of various organ systems.
35116628	1	0	gly	glycoprotein	149:160	arg1	Neuregulin					115:124	BACKGROUND Neuregulin 1	104:126	BACKGROUND Neuregulin 1 (NRG1)	104:133	BACKGROUND Neuregulin 1 (NRG1) is a membrane glycoprotein mediating cell-to-cell signaling and has a crucial role in the growth and development of various organ systems.
35116628	2	1	theme	V600E	337:341	arg1	status					350:355	BRAF V600E mutant status	332:355	BRAF V600E mutant status in papillary thyroid cancer (PTC) patients	332:398	Our study explored its diagnostic value in distinguishing BRAF V600E mutant status in papillary thyroid cancer (PTC) patients by analyzing multiple glycan patterns of serum NRG1 through lectin assays.
35116628	6	2	theme	glycan	855:860	arg1	profiles					862:869	got glycan profiles	851:869	RESULTS We got glycan profiles of 14 lectins, including GNL, GSL2, AAL, BPL, ECL, CAL, NML, HHL, PHA-L, RCA-I, ConA, DBA, PWA and LEL.	840:973	RESULTS We got glycan profiles of 14 lectins, including GNL, GSL2, AAL, BPL, ECL, CAL, NML, HHL, PHA-L, RCA-I, ConA, DBA, PWA and LEL.
35116628	9	3	theme	BRAF	1360:1363	arg1	controls					1379:1386	BRAF wild type PTC controls	1360:1386	BRAF wild type PTC controls	1360:1386	Both AAL ELISA and protein ELISA assays showed that the fucosylated structures of NRG1 had a remarkable increase in BRAF V600E mutant PTC patients compared with BRAF wild type PTC controls.
35116628	9	4	contain	had	1286:1288	arg1	structures					1267:1276	the fucosylated structures	1251:1276	the fucosylated structures of NRG1	1251:1284	Both AAL ELISA and protein ELISA assays showed that the fucosylated structures of NRG1 had a remarkable increase in BRAF V600E mutant PTC patients compared with BRAF wild type PTC controls.
35116628	9	4	contain	had	1286:1288	arg2	increase					1303:1310	a remarkable increase	1290:1310	a remarkable increase in BRAF V600E mutant PTC patients	1290:1344	Both AAL ELISA and protein ELISA assays showed that the fucosylated structures of NRG1 had a remarkable increase in BRAF V600E mutant PTC patients compared with BRAF wild type PTC controls.
35116628	10	5	theme	new	1420:1422	arg1	light					1424:1428	a new light	1418:1428	a new light	1418:1428	CONCLUSIONS This study sheds a new light on the role of NRG1 glycosylation in PTC.
35116628	1	6	theme	various	251:257	arg1	systems					265:271	various organ systems	251:271	various organ systems	251:271	BACKGROUND Neuregulin 1 (NRG1) is a membrane glycoprotein mediating cell-to-cell signaling and has a crucial role in the growth and development of various organ systems.
35116628	9	7	theme	type	1370:1373	arg1	controls					1379:1386	BRAF wild type PTC controls	1360:1386	BRAF wild type PTC controls	1360:1386	Both AAL ELISA and protein ELISA assays showed that the fucosylated structures of NRG1 had a remarkable increase in BRAF V600E mutant PTC patients compared with BRAF wild type PTC controls.
35116628	11	8	theme	BRAF	1581:1584	arg1	type					1591:1594	BRAF wild type	1581:1594	BRAF wild type negative fine needle aspiration results	1581:1634	NRG1 could serve as a supplementary glycobiomarker for BRAF indicator in discrimination of PTC patients with BRAF wild type negative fine needle aspiration results.
35116628	3	9	theme	PTC	513:515	arg1	patients					517:524	PTC patients	513:524	PTC patients	513:524	METHODS We first extracted serum from PTC patients and tested BRAF V600E mutation by immunohistochemical (IHC) staining.
35116628	3	10	dep	immunohistochemical	560:578	arg1	IHC					581:583	IHC	581:583	IHC	581:583	METHODS We first extracted serum from PTC patients and tested BRAF V600E mutation by immunohistochemical (IHC) staining.
35116628	4	11	theme	lectin	651:656	arg1	blot					658:661	lectin blot	651:661	lectin blot	651:661	Then we applied antibody overlay lectin microarray and lectin blot to detect glycol-alterations of NRG1.
35116628	2	12	from	status	350:355	arg1	patients					391:398	papillary thyroid cancer (PTC) patients	360:398	papillary thyroid cancer (PTC) patients	360:398	Our study explored its diagnostic value in distinguishing BRAF V600E mutant status in papillary thyroid cancer (PTC) patients by analyzing multiple glycan patterns of serum NRG1 through lectin assays.
35116628	2	12	from	status	350:355	arg1	PTC					386:388	PTC	386:388	PTC	386:388	Our study explored its diagnostic value in distinguishing BRAF V600E mutant status in papillary thyroid cancer (PTC) patients by analyzing multiple glycan patterns of serum NRG1 through lectin assays.
35116628	3	13	theme	BRAF	537:540	arg1	mutation					548:555	BRAF V600E mutation	537:555	BRAF V600E mutation	537:555	METHODS We first extracted serum from PTC patients and tested BRAF V600E mutation by immunohistochemical (IHC) staining.
35116628	5	14	theme	fucosylation	801:812	arg1	level					814:818	the protein fucosylation level	789:818	the protein fucosylation level of NRG1 (Fuc-NRG1)	789:837	Then Aleuria aurantia lectin (AAL) ELISA was performed according to ELISA index to test the protein fucosylation level of NRG1 (Fuc-NRG1).
35116628	11	15	theme	wild	1586:1589	arg1	type					1591:1594	BRAF wild type	1581:1594	BRAF wild type negative fine needle aspiration results	1581:1634	NRG1 could serve as a supplementary glycobiomarker for BRAF indicator in discrimination of PTC patients with BRAF wild type negative fine needle aspiration results.
35116628	4	16	theme	overlay	621:627	arg1	microarray					636:645	antibody overlay lectin microarray	612:645	antibody overlay lectin microarray	612:645	Then we applied antibody overlay lectin microarray and lectin blot to detect glycol-alterations of NRG1.
35116628	1	17	theme	crucial	205:211	arg1	role					213:216	a crucial role	203:216	a crucial role	203:216	BACKGROUND Neuregulin 1 (NRG1) is a membrane glycoprotein mediating cell-to-cell signaling and has a crucial role in the growth and development of various organ systems.
35116628	4	18	theme	NRG1	695:698	arg1	glycol-alterations					673:690	glycol-alterations	673:690	glycol-alterations of NRG1	673:698	Then we applied antibody overlay lectin microarray and lectin blot to detect glycol-alterations of NRG1.
35116628	3	19	theme	immunohistochemical	560:578	arg1	staining					586:593	immunohistochemical (IHC) staining	560:593	immunohistochemical (IHC) staining	560:593	METHODS We first extracted serum from PTC patients and tested BRAF V600E mutation by immunohistochemical (IHC) staining.
35116628	9	20	theme	AAL	1204:1206	arg1	ELISA					1208:1212	AAL ELISA	1204:1212	AAL ELISA	1204:1212	Both AAL ELISA and protein ELISA assays showed that the fucosylated structures of NRG1 had a remarkable increase in BRAF V600E mutant PTC patients compared with BRAF wild type PTC controls.
35116628	10	21	from	role	1437:1440	arg1	PTC					1467:1469	PTC	1467:1469	PTC	1467:1469	CONCLUSIONS This study sheds a new light on the role of NRG1 glycosylation in PTC.
35116628	6	22	theme	lectins	877:883	arg1	profiles					862:869	got glycan profiles	851:869	RESULTS We got glycan profiles of 14 lectins, including GNL, GSL2, AAL, BPL, ECL, CAL, NML, HHL, PHA-L, RCA-I, ConA, DBA, PWA and LEL.	840:973	RESULTS We got glycan profiles of 14 lectins, including GNL, GSL2, AAL, BPL, ECL, CAL, NML, HHL, PHA-L, RCA-I, ConA, DBA, PWA and LEL.
35116628	0	23	theme	serum	64:68	arg1	NRG1					70:73	serum NRG1	64:73	serum NRG1	64:73	Multiple lectin assays in detecting glycol-alteration status of serum NRG1 in papillary thyroid cancer.
35116628	7	24	contain	had	1032:1034	arg1	HHL					1012:1014	HHL	1012:1014	HHL	1012:1014	Six of them, namely, GSL2, BPL, NML, HHL, PHA-L and LEL, had significantly increased binding affinity capacity in BRAF(+) PTC compared with BRAF(-) PTC controls.
35116628	7	24	contain	had	1032:1034	arg1	Six					975:977	Six	975:977	Six	975:977	Six of them, namely, GSL2, BPL, NML, HHL, PHA-L and LEL, had significantly increased binding affinity capacity in BRAF(+) PTC compared with BRAF(-) PTC controls.
35116628	7	24	contain	had	1032:1034	arg1	GSL2					996:999	GSL2	996:999	GSL2	996:999	Six of them, namely, GSL2, BPL, NML, HHL, PHA-L and LEL, had significantly increased binding affinity capacity in BRAF(+) PTC compared with BRAF(-) PTC controls.
35116628	7	24	contain	had	1032:1034	arg2	capacity					1077:1084	significantly increased binding affinity capacity	1036:1084	significantly increased binding affinity capacity in BRAF(+) PTC	1036:1099	Six of them, namely, GSL2, BPL, NML, HHL, PHA-L and LEL, had significantly increased binding affinity capacity in BRAF(+) PTC compared with BRAF(-) PTC controls.
35116628	7	24	contain	had	1032:1034	arg1	BPL					1002:1004	BPL	1002:1004	BPL	1002:1004	Six of them, namely, GSL2, BPL, NML, HHL, PHA-L and LEL, had significantly increased binding affinity capacity in BRAF(+) PTC compared with BRAF(-) PTC controls.
35116628	7	24	contain	had	1032:1034	arg1	PHA-L					1017:1021	PHA-L	1017:1021	PHA-L	1017:1021	Six of them, namely, GSL2, BPL, NML, HHL, PHA-L and LEL, had significantly increased binding affinity capacity in BRAF(+) PTC compared with BRAF(-) PTC controls.
35116628	7	24	contain	had	1032:1034	arg1	them					982:985	them	982:985	them	982:985	Six of them, namely, GSL2, BPL, NML, HHL, PHA-L and LEL, had significantly increased binding affinity capacity in BRAF(+) PTC compared with BRAF(-) PTC controls.
35116628	7	24	contain	had	1032:1034	arg1	LEL					1027:1029	LEL	1027:1029	LEL	1027:1029	Six of them, namely, GSL2, BPL, NML, HHL, PHA-L and LEL, had significantly increased binding affinity capacity in BRAF(+) PTC compared with BRAF(-) PTC controls.
35116628	7	24	contain	had	1032:1034	arg1	NML					1007:1009	NML	1007:1009	NML	1007:1009	Six of them, namely, GSL2, BPL, NML, HHL, PHA-L and LEL, had significantly increased binding affinity capacity in BRAF(+) PTC compared with BRAF(-) PTC controls.
35116628	9	25	theme	ELISA	1226:1230	arg1	assays					1232:1237	Both AAL ELISA and protein ELISA assays	1199:1237	Both AAL ELISA and protein ELISA assays	1199:1237	Both AAL ELISA and protein ELISA assays showed that the fucosylated structures of NRG1 had a remarkable increase in BRAF V600E mutant PTC patients compared with BRAF wild type PTC controls.
35116628	1	26	theme	membrane	140:147	arg1	glycoprotein					149:160	a membrane glycoprotein	138:160	a membrane glycoprotein mediating cell-to-cell signaling	138:193	BACKGROUND Neuregulin 1 (NRG1) is a membrane glycoprotein mediating cell-to-cell signaling and has a crucial role in the growth and development of various organ systems.
35116628	1	26	theme	membrane	140:147	arg1	Neuregulin					115:124	BACKGROUND Neuregulin 1	104:126	BACKGROUND Neuregulin 1 (NRG1)	104:133	BACKGROUND Neuregulin 1 (NRG1) is a membrane glycoprotein mediating cell-to-cell signaling and has a crucial role in the growth and development of various organ systems.
35116628	0	27	theme	thyroid	88:94	arg1	cancer					96:101	papillary thyroid cancer	78:101	papillary thyroid cancer	78:101	Multiple lectin assays in detecting glycol-alteration status of serum NRG1 in papillary thyroid cancer.
35116628	0	28	from	status	54:59	arg1	cancer					96:101	papillary thyroid cancer	78:101	papillary thyroid cancer	78:101	Multiple lectin assays in detecting glycol-alteration status of serum NRG1 in papillary thyroid cancer.
35116628	11	29	theme	type	1591:1594	arg1	results					1628:1634	BRAF wild type negative fine needle aspiration results	1581:1634	BRAF wild type negative fine needle aspiration results	1581:1634	NRG1 could serve as a supplementary glycobiomarker for BRAF indicator in discrimination of PTC patients with BRAF wild type negative fine needle aspiration results.
35116628	3	30	dep	METHODS	475:481	arg1	extracted					492:500	extracted	492:500	first extracted serum from PTC patients	486:524	METHODS We first extracted serum from PTC patients and tested BRAF V600E mutation by immunohistochemical (IHC) staining.
35116628	3	30	dep	METHODS	475:481	arg1	tested					530:535	tested	530:535	tested BRAF V600E mutation by immunohistochemical (IHC) staining	530:593	METHODS We first extracted serum from PTC patients and tested BRAF V600E mutation by immunohistochemical (IHC) staining.
35116628	7	31	theme	BRAF	1115:1118	arg1	controls					1127:1134	BRAF(-) PTC controls	1115:1134	BRAF(-) PTC controls	1115:1134	Six of them, namely, GSL2, BPL, NML, HHL, PHA-L and LEL, had significantly increased binding affinity capacity in BRAF(+) PTC compared with BRAF(-) PTC controls.
35116628	11	32	from	indicator	1532:1540	arg1	discrimination					1545:1558	discrimination	1545:1558	discrimination of PTC patients with BRAF wild type negative fine needle aspiration results	1545:1634	NRG1 could serve as a supplementary glycobiomarker for BRAF indicator in discrimination of PTC patients with BRAF wild type negative fine needle aspiration results.
35116628	2	33	theme	thyroid	370:376	arg1	patients					391:398	papillary thyroid cancer (PTC) patients	360:398	papillary thyroid cancer (PTC) patients	360:398	Our study explored its diagnostic value in distinguishing BRAF V600E mutant status in papillary thyroid cancer (PTC) patients by analyzing multiple glycan patterns of serum NRG1 through lectin assays.
35116628	2	33	theme	thyroid	370:376	arg1	PTC					386:388	PTC	386:388	PTC	386:388	Our study explored its diagnostic value in distinguishing BRAF V600E mutant status in papillary thyroid cancer (PTC) patients by analyzing multiple glycan patterns of serum NRG1 through lectin assays.
35116628	0	34	theme	Multiple	0:7	arg1	assays					16:21	Multiple lectin assays	0:21	Multiple lectin assays in detecting glycol-alteration status of serum NRG1 in papillary thyroid cancer	0:101	Multiple lectin assays in detecting glycol-alteration status of serum NRG1 in papillary thyroid cancer.
35116628	1	35	theme	organ	259:263	arg1	systems					265:271	various organ systems	251:271	various organ systems	251:271	BACKGROUND Neuregulin 1 (NRG1) is a membrane glycoprotein mediating cell-to-cell signaling and has a crucial role in the growth and development of various organ systems.
35116628	9	36	theme	NRG1	1281:1284	arg1	structures					1267:1276	the fucosylated structures	1251:1276	the fucosylated structures of NRG1	1251:1284	Both AAL ELISA and protein ELISA assays showed that the fucosylated structures of NRG1 had a remarkable increase in BRAF V600E mutant PTC patients compared with BRAF wild type PTC controls.
35116628	2	37	theme	multiple	413:420	arg1	patterns					429:436	multiple glycan patterns	413:436	multiple glycan patterns of serum NRG1	413:450	Our study explored its diagnostic value in distinguishing BRAF V600E mutant status in papillary thyroid cancer (PTC) patients by analyzing multiple glycan patterns of serum NRG1 through lectin assays.
35116628	1	38	theme	BACKGROUND	104:113	arg1	Neuregulin					115:124	BACKGROUND Neuregulin 1	104:126	BACKGROUND Neuregulin 1 (NRG1)	104:133	BACKGROUND Neuregulin 1 (NRG1) is a membrane glycoprotein mediating cell-to-cell signaling and has a crucial role in the growth and development of various organ systems.
35116628	1	38	theme	BACKGROUND	104:113	arg1	NRG1					129:132	NRG1	129:132	NRG1	129:132	BACKGROUND Neuregulin 1 (NRG1) is a membrane glycoprotein mediating cell-to-cell signaling and has a crucial role in the growth and development of various organ systems.
35116628	1	38	theme	BACKGROUND	104:113	arg1	glycoprotein					149:160	a membrane glycoprotein	138:160	a membrane glycoprotein mediating cell-to-cell signaling	138:193	BACKGROUND Neuregulin 1 (NRG1) is a membrane glycoprotein mediating cell-to-cell signaling and has a crucial role in the growth and development of various organ systems.
35116628	9	39	gly	fucosylated	1255:1265	arg1	structures					1267:1276	the fucosylated structures	1251:1276	the fucosylated structures of NRG1	1251:1284	Both AAL ELISA and protein ELISA assays showed that the fucosylated structures of NRG1 had a remarkable increase in BRAF V600E mutant PTC patients compared with BRAF wild type PTC controls.
35116628	11	40	theme	fine	1605:1608	arg1	aspiration					1617:1626	negative fine needle aspiration	1596:1626	BRAF wild type negative fine needle aspiration results	1581:1634	NRG1 could serve as a supplementary glycobiomarker for BRAF indicator in discrimination of PTC patients with BRAF wild type negative fine needle aspiration results.
35116628	2	41	theme	serum	441:445	arg1	NRG1					447:450	serum NRG1	441:450	serum NRG1	441:450	Our study explored its diagnostic value in distinguishing BRAF V600E mutant status in papillary thyroid cancer (PTC) patients by analyzing multiple glycan patterns of serum NRG1 through lectin assays.
35116628	6	42	dep	PWA	962:964	arg1	ECL					917:919	AAL, BPL, ECL, CAL, NML, HHL, PHA-L, RCA-I, ConA, DBA, PWA	907:964	ECL	917:919	RESULTS We got glycan profiles of 14 lectins, including GNL, GSL2, AAL, BPL, ECL, CAL, NML, HHL, PHA-L, RCA-I, ConA, DBA, PWA and LEL.
35116628	6	42	dep	PWA	962:964	arg1	HHL					932:934	AAL, BPL, ECL, CAL, NML, HHL, PHA-L, RCA-I, ConA, DBA, PWA	907:964	HHL	932:934	RESULTS We got glycan profiles of 14 lectins, including GNL, GSL2, AAL, BPL, ECL, CAL, NML, HHL, PHA-L, RCA-I, ConA, DBA, PWA and LEL.
35116628	6	42	dep	PWA	962:964	arg1	PHA-L					937:941	AAL, BPL, ECL, CAL, NML, HHL, PHA-L, RCA-I, ConA, DBA, PWA	907:964	PHA-L	937:941	RESULTS We got glycan profiles of 14 lectins, including GNL, GSL2, AAL, BPL, ECL, CAL, NML, HHL, PHA-L, RCA-I, ConA, DBA, PWA and LEL.
35116628	6	42	dep	PWA	962:964	arg1	DBA					957:959	AAL, BPL, ECL, CAL, NML, HHL, PHA-L, RCA-I, ConA, DBA, PWA	907:964	DBA	957:959	RESULTS We got glycan profiles of 14 lectins, including GNL, GSL2, AAL, BPL, ECL, CAL, NML, HHL, PHA-L, RCA-I, ConA, DBA, PWA and LEL.
35116628	6	42	dep	PWA	962:964	arg1	CAL					922:924	AAL, BPL, ECL, CAL, NML, HHL, PHA-L, RCA-I, ConA, DBA, PWA	907:964	CAL	922:924	RESULTS We got glycan profiles of 14 lectins, including GNL, GSL2, AAL, BPL, ECL, CAL, NML, HHL, PHA-L, RCA-I, ConA, DBA, PWA and LEL.
35116628	6	42	dep	PWA	962:964	arg1	NML					927:929	AAL, BPL, ECL, CAL, NML, HHL, PHA-L, RCA-I, ConA, DBA, PWA	907:964	NML	927:929	RESULTS We got glycan profiles of 14 lectins, including GNL, GSL2, AAL, BPL, ECL, CAL, NML, HHL, PHA-L, RCA-I, ConA, DBA, PWA and LEL.
35116628	6	42	dep	PWA	962:964	arg1	RCA-I					944:948	AAL, BPL, ECL, CAL, NML, HHL, PHA-L, RCA-I, ConA, DBA, PWA	907:964	RCA-I	944:948	RESULTS We got glycan profiles of 14 lectins, including GNL, GSL2, AAL, BPL, ECL, CAL, NML, HHL, PHA-L, RCA-I, ConA, DBA, PWA and LEL.
35116628	6	42	dep	PWA	962:964	arg1	BPL					912:914	AAL, BPL, ECL, CAL, NML, HHL, PHA-L, RCA-I, ConA, DBA, PWA	907:964	BPL	912:914	RESULTS We got glycan profiles of 14 lectins, including GNL, GSL2, AAL, BPL, ECL, CAL, NML, HHL, PHA-L, RCA-I, ConA, DBA, PWA and LEL.
35116628	6	42	dep	PWA	962:964	arg1	ConA					951:954	AAL, BPL, ECL, CAL, NML, HHL, PHA-L, RCA-I, ConA, DBA, PWA	907:964	ConA	951:954	RESULTS We got glycan profiles of 14 lectins, including GNL, GSL2, AAL, BPL, ECL, CAL, NML, HHL, PHA-L, RCA-I, ConA, DBA, PWA and LEL.
35116628	2	43	theme	BRAF	332:335	arg1	status					350:355	BRAF V600E mutant status	332:355	BRAF V600E mutant status in papillary thyroid cancer (PTC) patients	332:398	Our study explored its diagnostic value in distinguishing BRAF V600E mutant status in papillary thyroid cancer (PTC) patients by analyzing multiple glycan patterns of serum NRG1 through lectin assays.
35116628	7	44	theme	them	982:985	arg1	HHL					1012:1014	HHL	1012:1014	HHL	1012:1014	Six of them, namely, GSL2, BPL, NML, HHL, PHA-L and LEL, had significantly increased binding affinity capacity in BRAF(+) PTC compared with BRAF(-) PTC controls.
35116628	7	44	theme	them	982:985	arg1	Six					975:977	Six	975:977	Six	975:977	Six of them, namely, GSL2, BPL, NML, HHL, PHA-L and LEL, had significantly increased binding affinity capacity in BRAF(+) PTC compared with BRAF(-) PTC controls.
35116628	7	44	theme	them	982:985	arg1	GSL2					996:999	GSL2	996:999	GSL2	996:999	Six of them, namely, GSL2, BPL, NML, HHL, PHA-L and LEL, had significantly increased binding affinity capacity in BRAF(+) PTC compared with BRAF(-) PTC controls.
35116628	7	44	theme	them	982:985	arg1	BPL					1002:1004	BPL	1002:1004	BPL	1002:1004	Six of them, namely, GSL2, BPL, NML, HHL, PHA-L and LEL, had significantly increased binding affinity capacity in BRAF(+) PTC compared with BRAF(-) PTC controls.
35116628	7	44	theme	them	982:985	arg1	PHA-L					1017:1021	PHA-L	1017:1021	PHA-L	1017:1021	Six of them, namely, GSL2, BPL, NML, HHL, PHA-L and LEL, had significantly increased binding affinity capacity in BRAF(+) PTC compared with BRAF(-) PTC controls.
35116628	7	44	theme	them	982:985	arg1	them					982:985	them	982:985	them	982:985	Six of them, namely, GSL2, BPL, NML, HHL, PHA-L and LEL, had significantly increased binding affinity capacity in BRAF(+) PTC compared with BRAF(-) PTC controls.
35116628	7	44	theme	them	982:985	arg1	LEL					1027:1029	LEL	1027:1029	LEL	1027:1029	Six of them, namely, GSL2, BPL, NML, HHL, PHA-L and LEL, had significantly increased binding affinity capacity in BRAF(+) PTC compared with BRAF(-) PTC controls.
35116628	7	44	theme	them	982:985	arg1	NML					1007:1009	NML	1007:1009	NML	1007:1009	Six of them, namely, GSL2, BPL, NML, HHL, PHA-L and LEL, had significantly increased binding affinity capacity in BRAF(+) PTC compared with BRAF(-) PTC controls.
35116628	9	45	theme	BRAF	1315:1318	arg1	patients					1337:1344	BRAF V600E mutant PTC patients	1315:1344	BRAF V600E mutant PTC patients	1315:1344	Both AAL ELISA and protein ELISA assays showed that the fucosylated structures of NRG1 had a remarkable increase in BRAF V600E mutant PTC patients compared with BRAF wild type PTC controls.
35116628	11	46	theme	BRAF	1527:1530	arg1	indicator					1532:1540	BRAF indicator	1527:1540	BRAF indicator in discrimination of PTC patients with BRAF wild type negative fine needle aspiration results	1527:1634	NRG1 could serve as a supplementary glycobiomarker for BRAF indicator in discrimination of PTC patients with BRAF wild type negative fine needle aspiration results.
35116628	9	47	theme	mutant	1326:1331	arg1	patients					1337:1344	BRAF V600E mutant PTC patients	1315:1344	BRAF V600E mutant PTC patients	1315:1344	Both AAL ELISA and protein ELISA assays showed that the fucosylated structures of NRG1 had a remarkable increase in BRAF V600E mutant PTC patients compared with BRAF wild type PTC controls.
35116628	7	48	theme	affinity	1068:1075	arg1	capacity					1077:1084	significantly increased binding affinity capacity	1036:1084	significantly increased binding affinity capacity in BRAF(+) PTC	1036:1099	Six of them, namely, GSL2, BPL, NML, HHL, PHA-L and LEL, had significantly increased binding affinity capacity in BRAF(+) PTC compared with BRAF(-) PTC controls.
35116628	11	49	theme	patients	1567:1574	arg1	discrimination					1545:1558	discrimination	1545:1558	discrimination of PTC patients with BRAF wild type negative fine needle aspiration results	1545:1634	NRG1 could serve as a supplementary glycobiomarker for BRAF indicator in discrimination of PTC patients with BRAF wild type negative fine needle aspiration results.
35116628	7	50	theme	increased	1050:1058	arg1	capacity					1077:1084	significantly increased binding affinity capacity	1036:1084	significantly increased binding affinity capacity in BRAF(+) PTC	1036:1099	Six of them, namely, GSL2, BPL, NML, HHL, PHA-L and LEL, had significantly increased binding affinity capacity in BRAF(+) PTC compared with BRAF(-) PTC controls.
35116628	6	51	dep	We	848:849	arg1	profiles					862:869	got glycan profiles	851:869	RESULTS We got glycan profiles of 14 lectins, including GNL, GSL2, AAL, BPL, ECL, CAL, NML, HHL, PHA-L, RCA-I, ConA, DBA, PWA and LEL.	840:973	RESULTS We got glycan profiles of 14 lectins, including GNL, GSL2, AAL, BPL, ECL, CAL, NML, HHL, PHA-L, RCA-I, ConA, DBA, PWA and LEL.
35116628	2	52	theme	mutant	343:348	arg1	status					350:355	BRAF V600E mutant status	332:355	BRAF V600E mutant status in papillary thyroid cancer (PTC) patients	332:398	Our study explored its diagnostic value in distinguishing BRAF V600E mutant status in papillary thyroid cancer (PTC) patients by analyzing multiple glycan patterns of serum NRG1 through lectin assays.
35116628	11	53	with	patients	1567:1574	arg1	results					1628:1634	BRAF wild type negative fine needle aspiration results	1581:1634	BRAF wild type negative fine needle aspiration results	1581:1634	NRG1 could serve as a supplementary glycobiomarker for BRAF indicator in discrimination of PTC patients with BRAF wild type negative fine needle aspiration results.
35116628	10	54	theme	NRG1	1445:1448	arg1	glycosylation					1450:1462	NRG1 glycosylation	1445:1462	NRG1 glycosylation	1445:1462	CONCLUSIONS This study sheds a new light on the role of NRG1 glycosylation in PTC.
35116628	3	55	theme	V600E	542:546	arg1	mutation					548:555	BRAF V600E mutation	537:555	BRAF V600E mutation	537:555	METHODS We first extracted serum from PTC patients and tested BRAF V600E mutation by immunohistochemical (IHC) staining.
35116628	6	56	theme	got	851:853	arg1	profiles					862:869	got glycan profiles	851:869	RESULTS We got glycan profiles of 14 lectins, including GNL, GSL2, AAL, BPL, ECL, CAL, NML, HHL, PHA-L, RCA-I, ConA, DBA, PWA and LEL.	840:973	RESULTS We got glycan profiles of 14 lectins, including GNL, GSL2, AAL, BPL, ECL, CAL, NML, HHL, PHA-L, RCA-I, ConA, DBA, PWA and LEL.
35116628	9	57	theme	wild	1365:1368	arg1	controls					1379:1386	BRAF wild type PTC controls	1360:1386	BRAF wild type PTC controls	1360:1386	Both AAL ELISA and protein ELISA assays showed that the fucosylated structures of NRG1 had a remarkable increase in BRAF V600E mutant PTC patients compared with BRAF wild type PTC controls.
35116628	5	58	theme	NRG1	823:826	arg1	level					814:818	the protein fucosylation level	789:818	the protein fucosylation level of NRG1 (Fuc-NRG1)	789:837	Then Aleuria aurantia lectin (AAL) ELISA was performed according to ELISA index to test the protein fucosylation level of NRG1 (Fuc-NRG1).
35116628	5	59	theme	ELISA	769:773	arg1	index					775:779	ELISA index	769:779	ELISA index	769:779	Then Aleuria aurantia lectin (AAL) ELISA was performed according to ELISA index to test the protein fucosylation level of NRG1 (Fuc-NRG1).
35116628	9	60	theme	PTC	1375:1377	arg1	controls					1379:1386	BRAF wild type PTC controls	1360:1386	BRAF wild type PTC controls	1360:1386	Both AAL ELISA and protein ELISA assays showed that the fucosylated structures of NRG1 had a remarkable increase in BRAF V600E mutant PTC patients compared with BRAF wild type PTC controls.
35116628	4	61	theme	lectin	629:634	arg1	microarray					636:645	antibody overlay lectin microarray	612:645	antibody overlay lectin microarray	612:645	Then we applied antibody overlay lectin microarray and lectin blot to detect glycol-alterations of NRG1.
35116628	2	62	theme	diagnostic	297:306	arg1	value					308:312	its diagnostic value	293:312	its diagnostic value	293:312	Our study explored its diagnostic value in distinguishing BRAF V600E mutant status in papillary thyroid cancer (PTC) patients by analyzing multiple glycan patterns of serum NRG1 through lectin assays.
35116628	1	63	theme	cell-to-cell	172:183	arg1	signaling					185:193	cell-to-cell signaling	172:193	cell-to-cell signaling	172:193	BACKGROUND Neuregulin 1 (NRG1) is a membrane glycoprotein mediating cell-to-cell signaling and has a crucial role in the growth and development of various organ systems.
35116628	7	64	from	capacity	1077:1084	arg1	BRAF					1089:1092	BRAF	1089:1092	BRAF	1089:1092	Six of them, namely, GSL2, BPL, NML, HHL, PHA-L and LEL, had significantly increased binding affinity capacity in BRAF(+) PTC compared with BRAF(-) PTC controls.
35116628	7	64	from	capacity	1077:1084	arg1	PTC					1097:1099	PTC	1097:1099	PTC	1097:1099	Six of them, namely, GSL2, BPL, NML, HHL, PHA-L and LEL, had significantly increased binding affinity capacity in BRAF(+) PTC compared with BRAF(-) PTC controls.
35116628	5	65	theme	protein	793:799	arg1	level					814:818	the protein fucosylation level	789:818	the protein fucosylation level of NRG1 (Fuc-NRG1)	789:837	Then Aleuria aurantia lectin (AAL) ELISA was performed according to ELISA index to test the protein fucosylation level of NRG1 (Fuc-NRG1).
35116628	8	66	theme	BRAF	1180:1183	arg1	group					1192:1196	BRAF(+) PTC group	1180:1196	BRAF(+) PTC group	1180:1196	LEL, BPL and NML tended to bind to NRG1 in BRAF(+) PTC group.
35116628	4	67	theme	antibody	612:619	arg1	microarray					636:645	antibody overlay lectin microarray	612:645	antibody overlay lectin microarray	612:645	Then we applied antibody overlay lectin microarray and lectin blot to detect glycol-alterations of NRG1.
35116628	8	68	theme	PTC	1188:1190	arg1	group					1192:1196	BRAF(+) PTC group	1180:1196	BRAF(+) PTC group	1180:1196	LEL, BPL and NML tended to bind to NRG1 in BRAF(+) PTC group.
35116628	11	69	theme	supplementary	1494:1506	arg1	NRG1					1472:1475	NRG1	1472:1475	NRG1	1472:1475	NRG1 could serve as a supplementary glycobiomarker for BRAF indicator in discrimination of PTC patients with BRAF wild type negative fine needle aspiration results.
35116628	11	69	theme	supplementary	1494:1506	arg1	glycobiomarker					1508:1521	a supplementary glycobiomarker	1492:1521	a supplementary glycobiomarker for BRAF indicator in discrimination of PTC patients with BRAF wild type negative fine needle aspiration results	1492:1634	NRG1 could serve as a supplementary glycobiomarker for BRAF indicator in discrimination of PTC patients with BRAF wild type negative fine needle aspiration results.
35116628	9	70	theme	ELISA	1208:1212	arg1	assays					1232:1237	Both AAL ELISA and protein ELISA assays	1199:1237	Both AAL ELISA and protein ELISA assays	1199:1237	Both AAL ELISA and protein ELISA assays showed that the fucosylated structures of NRG1 had a remarkable increase in BRAF V600E mutant PTC patients compared with BRAF wild type PTC controls.
35116628	7	71	theme	PTC	1123:1125	arg1	controls					1127:1134	BRAF(-) PTC controls	1115:1134	BRAF(-) PTC controls	1115:1134	Six of them, namely, GSL2, BPL, NML, HHL, PHA-L and LEL, had significantly increased binding affinity capacity in BRAF(+) PTC compared with BRAF(-) PTC controls.
35116628	0	72	theme	NRG1	70:73	arg1	status					54:59	glycol-alteration status	36:59	glycol-alteration status of serum NRG1 in papillary thyroid cancer	36:101	Multiple lectin assays in detecting glycol-alteration status of serum NRG1 in papillary thyroid cancer.
35116628	9	73	theme	protein	1218:1224	arg1	ELISA					1226:1230	protein ELISA	1218:1230	protein ELISA	1218:1230	Both AAL ELISA and protein ELISA assays showed that the fucosylated structures of NRG1 had a remarkable increase in BRAF V600E mutant PTC patients compared with BRAF wild type PTC controls.
35116628	5	74	theme	aurantia	714:721	arg1	lectin					723:728	Aleuria aurantia lectin	706:728	Aleuria aurantia lectin (AAL) ELISA	706:740	Then Aleuria aurantia lectin (AAL) ELISA was performed according to ELISA index to test the protein fucosylation level of NRG1 (Fuc-NRG1).
35116628	5	74	theme	aurantia	714:721	arg1	AAL					731:733	AAL	731:733	AAL	731:733	Then Aleuria aurantia lectin (AAL) ELISA was performed according to ELISA index to test the protein fucosylation level of NRG1 (Fuc-NRG1).
35116628	0	75	theme	papillary	78:86	arg1	cancer					96:101	papillary thyroid cancer	78:101	papillary thyroid cancer	78:101	Multiple lectin assays in detecting glycol-alteration status of serum NRG1 in papillary thyroid cancer.
35116628	11	76	theme	PTC	1563:1565	arg1	patients					1567:1574	PTC patients	1563:1574	PTC patients with BRAF wild type negative fine needle aspiration results	1563:1634	NRG1 could serve as a supplementary glycobiomarker for BRAF indicator in discrimination of PTC patients with BRAF wild type negative fine needle aspiration results.
35116628	2	77	theme	lectin	460:465	arg1	assays					467:472	lectin assays	460:472	lectin assays	460:472	Our study explored its diagnostic value in distinguishing BRAF V600E mutant status in papillary thyroid cancer (PTC) patients by analyzing multiple glycan patterns of serum NRG1 through lectin assays.
35116628	3	78	from	patients	517:524	arg1	serum					502:506	serum	502:506	serum from PTC patients	502:524	METHODS We first extracted serum from PTC patients and tested BRAF V600E mutation by immunohistochemical (IHC) staining.
35116628	5	79	theme	Aleuria	706:712	arg1	lectin					723:728	Aleuria aurantia lectin	706:728	Aleuria aurantia lectin (AAL) ELISA	706:740	Then Aleuria aurantia lectin (AAL) ELISA was performed according to ELISA index to test the protein fucosylation level of NRG1 (Fuc-NRG1).
35116628	5	79	theme	Aleuria	706:712	arg1	AAL					731:733	AAL	731:733	AAL	731:733	Then Aleuria aurantia lectin (AAL) ELISA was performed according to ELISA index to test the protein fucosylation level of NRG1 (Fuc-NRG1).
35116628	10	80	theme	glycosylation	1450:1462	arg1	role					1437:1440	the role	1433:1440	the role of NRG1 glycosylation in PTC	1433:1469	CONCLUSIONS This study sheds a new light on the role of NRG1 glycosylation in PTC.
35116628	5	81	theme	lectin	723:728	arg1	ELISA					736:740	Aleuria aurantia lectin (AAL) ELISA	706:740	Aleuria aurantia lectin (AAL) ELISA	706:740	Then Aleuria aurantia lectin (AAL) ELISA was performed according to ELISA index to test the protein fucosylation level of NRG1 (Fuc-NRG1).
35116628	9	82	theme	fucosylated	1255:1265	arg1	structures					1267:1276	the fucosylated structures	1251:1276	the fucosylated structures of NRG1	1251:1284	Both AAL ELISA and protein ELISA assays showed that the fucosylated structures of NRG1 had a remarkable increase in BRAF V600E mutant PTC patients compared with BRAF wild type PTC controls.
35116628	2	83	theme	cancer	378:383	arg1	patients					391:398	papillary thyroid cancer (PTC) patients	360:398	papillary thyroid cancer (PTC) patients	360:398	Our study explored its diagnostic value in distinguishing BRAF V600E mutant status in papillary thyroid cancer (PTC) patients by analyzing multiple glycan patterns of serum NRG1 through lectin assays.
35116628	2	83	theme	cancer	378:383	arg1	PTC					386:388	PTC	386:388	PTC	386:388	Our study explored its diagnostic value in distinguishing BRAF V600E mutant status in papillary thyroid cancer (PTC) patients by analyzing multiple glycan patterns of serum NRG1 through lectin assays.
35116628	0	84	theme	lectin	9:14	arg1	assays					16:21	Multiple lectin assays	0:21	Multiple lectin assays in detecting glycol-alteration status of serum NRG1 in papillary thyroid cancer	0:101	Multiple lectin assays in detecting glycol-alteration status of serum NRG1 in papillary thyroid cancer.
35116628	2	85	theme	glycan	422:427	arg1	patterns					429:436	multiple glycan patterns	413:436	multiple glycan patterns of serum NRG1	413:450	Our study explored its diagnostic value in distinguishing BRAF V600E mutant status in papillary thyroid cancer (PTC) patients by analyzing multiple glycan patterns of serum NRG1 through lectin assays.
35116628	10	86	dep	CONCLUSIONS	1389:1399	arg1	sheds					1412:1416	sheds	1412:1416	sheds a new light on the role of NRG1 glycosylation in PTC	1412:1469	CONCLUSIONS This study sheds a new light on the role of NRG1 glycosylation in PTC.
35116628	2	87	theme	papillary	360:368	arg1	patients					391:398	papillary thyroid cancer (PTC) patients	360:398	papillary thyroid cancer (PTC) patients	360:398	Our study explored its diagnostic value in distinguishing BRAF V600E mutant status in papillary thyroid cancer (PTC) patients by analyzing multiple glycan patterns of serum NRG1 through lectin assays.
35116628	2	87	theme	papillary	360:368	arg1	PTC					386:388	PTC	386:388	PTC	386:388	Our study explored its diagnostic value in distinguishing BRAF V600E mutant status in papillary thyroid cancer (PTC) patients by analyzing multiple glycan patterns of serum NRG1 through lectin assays.
35116628	1	88	theme	systems	265:271	arg1	development					236:246	development	236:246	development	236:246	BACKGROUND Neuregulin 1 (NRG1) is a membrane glycoprotein mediating cell-to-cell signaling and has a crucial role in the growth and development of various organ systems.
35116628	1	88	theme	systems	265:271	arg1	growth					225:230	growth	225:230	growth	225:230	BACKGROUND Neuregulin 1 (NRG1) is a membrane glycoprotein mediating cell-to-cell signaling and has a crucial role in the growth and development of various organ systems.
35116628	0	89	theme	glycol-alteration	36:52	arg1	status					54:59	glycol-alteration status	36:59	glycol-alteration status of serum NRG1 in papillary thyroid cancer	36:101	Multiple lectin assays in detecting glycol-alteration status of serum NRG1 in papillary thyroid cancer.
35116628	9	90	theme	remarkable	1292:1301	arg1	increase					1303:1310	a remarkable increase	1290:1310	a remarkable increase in BRAF V600E mutant PTC patients	1290:1344	Both AAL ELISA and protein ELISA assays showed that the fucosylated structures of NRG1 had a remarkable increase in BRAF V600E mutant PTC patients compared with BRAF wild type PTC controls.
35116628	9	91	from	increase	1303:1310	arg1	patients					1337:1344	BRAF V600E mutant PTC patients	1315:1344	BRAF V600E mutant PTC patients	1315:1344	Both AAL ELISA and protein ELISA assays showed that the fucosylated structures of NRG1 had a remarkable increase in BRAF V600E mutant PTC patients compared with BRAF wild type PTC controls.
35116628	2	92	theme	NRG1	447:450	arg1	patterns					429:436	multiple glycan patterns	413:436	multiple glycan patterns of serum NRG1	413:450	Our study explored its diagnostic value in distinguishing BRAF V600E mutant status in papillary thyroid cancer (PTC) patients by analyzing multiple glycan patterns of serum NRG1 through lectin assays.
35116628	11	93	theme	needle	1610:1615	arg1	aspiration					1617:1626	negative fine needle aspiration	1596:1626	BRAF wild type negative fine needle aspiration results	1581:1634	NRG1 could serve as a supplementary glycobiomarker for BRAF indicator in discrimination of PTC patients with BRAF wild type negative fine needle aspiration results.
35116628	11	94	dep	type	1591:1594	arg1	aspiration					1617:1626	negative fine needle aspiration	1596:1626	BRAF wild type negative fine needle aspiration results	1581:1634	NRG1 could serve as a supplementary glycobiomarker for BRAF indicator in discrimination of PTC patients with BRAF wild type negative fine needle aspiration results.
35116628	9	95	theme	V600E	1320:1324	arg1	patients					1337:1344	BRAF V600E mutant PTC patients	1315:1344	BRAF V600E mutant PTC patients	1315:1344	Both AAL ELISA and protein ELISA assays showed that the fucosylated structures of NRG1 had a remarkable increase in BRAF V600E mutant PTC patients compared with BRAF wild type PTC controls.
35116628	7	96	theme	binding	1060:1066	arg1	capacity					1077:1084	significantly increased binding affinity capacity	1036:1084	significantly increased binding affinity capacity in BRAF(+) PTC	1036:1099	Six of them, namely, GSL2, BPL, NML, HHL, PHA-L and LEL, had significantly increased binding affinity capacity in BRAF(+) PTC compared with BRAF(-) PTC controls.
35116628	6	97	dep	RESULTS	840:846	arg1	We					848:849	We	848:849	We	848:849	RESULTS We got glycan profiles of 14 lectins, including GNL, GSL2, AAL, BPL, ECL, CAL, NML, HHL, PHA-L, RCA-I, ConA, DBA, PWA and LEL.
35116628	1	98	contain	has	199:201	arg2	role					213:216	a crucial role	203:216	a crucial role	203:216	BACKGROUND Neuregulin 1 (NRG1) is a membrane glycoprotein mediating cell-to-cell signaling and has a crucial role in the growth and development of various organ systems.
35116628	1	98	contain	has	199:201	arg1	Neuregulin					115:124	BACKGROUND Neuregulin 1	104:126	BACKGROUND Neuregulin 1 (NRG1)	104:133	BACKGROUND Neuregulin 1 (NRG1) is a membrane glycoprotein mediating cell-to-cell signaling and has a crucial role in the growth and development of various organ systems.
35116628	1	98	contain	has	199:201	arg1	NRG1					129:132	NRG1	129:132	NRG1	129:132	BACKGROUND Neuregulin 1 (NRG1) is a membrane glycoprotein mediating cell-to-cell signaling and has a crucial role in the growth and development of various organ systems.
35116628	1	98	contain	has	199:201	arg1	glycoprotein					149:160	a membrane glycoprotein	138:160	a membrane glycoprotein mediating cell-to-cell signaling	138:193	BACKGROUND Neuregulin 1 (NRG1) is a membrane glycoprotein mediating cell-to-cell signaling and has a crucial role in the growth and development of various organ systems.
35116628	9	99	theme	PTC	1333:1335	arg1	patients					1337:1344	BRAF V600E mutant PTC patients	1315:1344	BRAF V600E mutant PTC patients	1315:1344	Both AAL ELISA and protein ELISA assays showed that the fucosylated structures of NRG1 had a remarkable increase in BRAF V600E mutant PTC patients compared with BRAF wild type PTC controls.
35116628	11	100	theme	negative	1596:1603	arg1	aspiration					1617:1626	negative fine needle aspiration	1596:1626	BRAF wild type negative fine needle aspiration results	1581:1634	NRG1 could serve as a supplementary glycobiomarker for BRAF indicator in discrimination of PTC patients with BRAF wild type negative fine needle aspiration results.
34324645	0	0	theme	zone	84:87	arg1	niches					100:105	young and aged subventricular zone neurogenic niches	54:105	young and aged subventricular zone neurogenic niches	54:105	Regulation of fractone heparan sulfate composition in young and aged subventricular zone neurogenic niches.
34324645	4	1	from	impact	804:809	arg1	neurogenesis					814:825	neurogenesis	814:825	neurogenesis	814:825	To further understand the conditions that regulate 6-O sulfation levels and its impact on neurogenesis, we used endosulfatase Sulf1 and Sulf2 double knockout (DKO) mice.
34324645	2	2	from	decline	440:446	arg1	neurogenesis					451:462	neurogenesis	451:462	neurogenesis	451:462	During aging, a decline in neurogenesis correlates with a change in the composition of heparan sulfate (HS) within fractones.
34324645	1	3	theme	heparin-binding	328:342	arg1	mechanism					344:352	a heparin-binding mechanism	326:352	a heparin-binding mechanism for neural stem cell (NSC) presentation, which promotes neurogenesis	326:421	Fractones, specialized extracellular matrix structures found in the subventricular zone (SVZ) neurogenic niche, can capture growth factors, such as basic fibroblast growth factor, from the extracellular milieu through a heparin-binding mechanism for neural stem cell (NSC) presentation, which promotes neurogenesis.
34324645	6	4	theme	mice	1180:1183	arg1	SVZ					1161:1163	the young and aged SVZ	1142:1163	the young and aged SVZ of Sulf1/2 DKO mice	1142:1183	While neurogenesis declined in the aged SVZ of both wild-type and Sulf1/2 DKO mice, we observed a larger number of neuroblasts in the young and aged SVZ of Sulf1/2 DKO mice.
34324645	0	5	from	Regulation	0:9	arg1	niches					100:105	young and aged subventricular zone neurogenic niches	54:105	young and aged subventricular zone neurogenic niches	54:105	Regulation of fractone heparan sulfate composition in young and aged subventricular zone neurogenic niches.
34324645	7	6	theme	6-O-sulfation	1235:1247	arg1	removal					1224:1230	the removal	1220:1230	the removal of 6-O-sulfation in fractones HS by endosulfatases	1220:1281	Together, these results show that the removal of 6-O-sulfation in fractones HS by endosulfatases inhibits neurogenesis in the SVZ.
34324645	6	7	theme	larger	1110:1115	arg1	number					1117:1122	a larger number	1108:1122	a larger number of neuroblasts	1108:1137	While neurogenesis declined in the aged SVZ of both wild-type and Sulf1/2 DKO mice, we observed a larger number of neuroblasts in the young and aged SVZ of Sulf1/2 DKO mice.
34324645	7	8	from	6-O-sulfation	1235:1247	arg1	HS					1262:1263	fractones HS	1252:1263	fractones HS	1252:1263	Together, these results show that the removal of 6-O-sulfation in fractones HS by endosulfatases inhibits neurogenesis in the SVZ.
34324645	0	9	theme	neurogenic	89:98	arg1	niches					100:105	young and aged subventricular zone neurogenic niches	54:105	young and aged subventricular zone neurogenic niches	54:105	Regulation of fractone heparan sulfate composition in young and aged subventricular zone neurogenic niches.
34324645	1	10	located	found	163:167	arg2	Fractones					108:116	Fractones	108:116	Fractones	108:116	Fractones, specialized extracellular matrix structures found in the subventricular zone (SVZ) neurogenic niche, can capture growth factors, such as basic fibroblast growth factor, from the extracellular milieu through a heparin-binding mechanism for neural stem cell (NSC) presentation, which promotes neurogenesis.
34324645	1	10	located	found	163:167	arg1	niche					213:217	the subventricular zone (SVZ) neurogenic niche	172:217	the subventricular zone (SVZ) neurogenic niche	172:217	Fractones, specialized extracellular matrix structures found in the subventricular zone (SVZ) neurogenic niche, can capture growth factors, such as basic fibroblast growth factor, from the extracellular milieu through a heparin-binding mechanism for neural stem cell (NSC) presentation, which promotes neurogenesis.
34324645	1	10	located	found	163:167	arg2	structures					152:161	specialized extracellular matrix structures	119:161	specialized extracellular matrix structures found in the subventricular zone (SVZ) neurogenic niche	119:217	Fractones, specialized extracellular matrix structures found in the subventricular zone (SVZ) neurogenic niche, can capture growth factors, such as basic fibroblast growth factor, from the extracellular milieu through a heparin-binding mechanism for neural stem cell (NSC) presentation, which promotes neurogenesis.
34324645	4	11	theme	endosulfatase	836:848	arg1	Sulf1					850:854	endosulfatase Sulf1	836:854	endosulfatase Sulf1	836:854	To further understand the conditions that regulate 6-O sulfation levels and its impact on neurogenesis, we used endosulfatase Sulf1 and Sulf2 double knockout (DKO) mice.
34324645	1	12	theme	basic	256:260	arg1	factor					280:285	basic fibroblast growth factor	256:285	basic fibroblast growth factor	256:285	Fractones, specialized extracellular matrix structures found in the subventricular zone (SVZ) neurogenic niche, can capture growth factors, such as basic fibroblast growth factor, from the extracellular milieu through a heparin-binding mechanism for neural stem cell (NSC) presentation, which promotes neurogenesis.
34324645	1	13	theme	neural	358:363	arg1	NSC					376:378	NSC	376:378	NSC	376:378	Fractones, specialized extracellular matrix structures found in the subventricular zone (SVZ) neurogenic niche, can capture growth factors, such as basic fibroblast growth factor, from the extracellular milieu through a heparin-binding mechanism for neural stem cell (NSC) presentation, which promotes neurogenesis.
34324645	1	13	theme	neural	358:363	arg1	cell					370:373	neural stem cell	358:373	neural stem cell (NSC) presentation	358:392	Fractones, specialized extracellular matrix structures found in the subventricular zone (SVZ) neurogenic niche, can capture growth factors, such as basic fibroblast growth factor, from the extracellular milieu through a heparin-binding mechanism for neural stem cell (NSC) presentation, which promotes neurogenesis.
34324645	6	14	theme	DKO	1176:1178	arg1	mice					1180:1183	Sulf1/2 DKO mice	1168:1183	Sulf1/2 DKO mice	1168:1183	While neurogenesis declined in the aged SVZ of both wild-type and Sulf1/2 DKO mice, we observed a larger number of neuroblasts in the young and aged SVZ of Sulf1/2 DKO mice.
34324645	5	15	theme	Sulf1/2	918:924	arg1	mice					930:933	Sulf1/2 DKO mice	918:933	Sulf1/2 DKO mice	918:933	Fractones in the SVZ of Sulf1/2 DKO mice showed immunoreactivity for the HS epitope, suggesting higher 6-O sulfation.
34324645	1	16	theme	subventricular	176:189	arg1	SVZ					197:199	SVZ	197:199	SVZ	197:199	Fractones, specialized extracellular matrix structures found in the subventricular zone (SVZ) neurogenic niche, can capture growth factors, such as basic fibroblast growth factor, from the extracellular milieu through a heparin-binding mechanism for neural stem cell (NSC) presentation, which promotes neurogenesis.
34324645	1	16	theme	subventricular	176:189	arg1	zone					191:194	the subventricular zone	172:194	the subventricular zone (SVZ) neurogenic niche	172:217	Fractones, specialized extracellular matrix structures found in the subventricular zone (SVZ) neurogenic niche, can capture growth factors, such as basic fibroblast growth factor, from the extracellular milieu through a heparin-binding mechanism for neural stem cell (NSC) presentation, which promotes neurogenesis.
34324645	1	17	theme	stem	365:368	arg1	NSC					376:378	NSC	376:378	NSC	376:378	Fractones, specialized extracellular matrix structures found in the subventricular zone (SVZ) neurogenic niche, can capture growth factors, such as basic fibroblast growth factor, from the extracellular milieu through a heparin-binding mechanism for neural stem cell (NSC) presentation, which promotes neurogenesis.
34324645	1	17	theme	stem	365:368	arg1	cell					370:373	neural stem cell	358:373	neural stem cell (NSC) presentation	358:392	Fractones, specialized extracellular matrix structures found in the subventricular zone (SVZ) neurogenic niche, can capture growth factors, such as basic fibroblast growth factor, from the extracellular milieu through a heparin-binding mechanism for neural stem cell (NSC) presentation, which promotes neurogenesis.
34324645	6	18	theme	Sulf1/2	1168:1174	arg1	mice					1180:1183	Sulf1/2 DKO mice	1168:1183	Sulf1/2 DKO mice	1168:1183	While neurogenesis declined in the aged SVZ of both wild-type and Sulf1/2 DKO mice, we observed a larger number of neuroblasts in the young and aged SVZ of Sulf1/2 DKO mice.
34324645	5	19	theme	DKO	926:928	arg1	mice					930:933	Sulf1/2 DKO mice	918:933	Sulf1/2 DKO mice	918:933	Fractones in the SVZ of Sulf1/2 DKO mice showed immunoreactivity for the HS epitope, suggesting higher 6-O sulfation.
34324645	7	20	from	HS	1262:1263	arg1	removal					1224:1230	the removal	1220:1230	the removal of 6-O-sulfation in fractones HS by endosulfatases	1220:1281	Together, these results show that the removal of 6-O-sulfation in fractones HS by endosulfatases inhibits neurogenesis in the SVZ.
34324645	1	21	theme	zone	191:194	arg1	niche					213:217	the subventricular zone (SVZ) neurogenic niche	172:217	the subventricular zone (SVZ) neurogenic niche	172:217	Fractones, specialized extracellular matrix structures found in the subventricular zone (SVZ) neurogenic niche, can capture growth factors, such as basic fibroblast growth factor, from the extracellular milieu through a heparin-binding mechanism for neural stem cell (NSC) presentation, which promotes neurogenesis.
34324645	1	22	theme	cell	370:373	arg1	presentation					381:392	neural stem cell (NSC) presentation	358:392	neural stem cell (NSC) presentation	358:392	Fractones, specialized extracellular matrix structures found in the subventricular zone (SVZ) neurogenic niche, can capture growth factors, such as basic fibroblast growth factor, from the extracellular milieu through a heparin-binding mechanism for neural stem cell (NSC) presentation, which promotes neurogenesis.
34324645	8	23	theme	cell	1492:1495	arg1	therapies					1497:1505	future stem cell therapies	1480:1505	future stem cell therapies	1480:1505	Our findings advance the current understanding regarding the extracellular environment that is best suited for NSCs to thrive, which is critical for the design of future stem cell therapies.
34324645	3	24	theme	young	701:705	arg1	brains					716:721	young and aged brains	701:721	young and aged brains	701:721	In this study, we used antibodies that recognize specific short oligosaccharides with varying sulfation to evaluate the HS composition in fractones in young and aged brains.
34324645	3	25	from	fractones	688:696	arg1	brains					716:721	young and aged brains	701:721	young and aged brains	701:721	In this study, we used antibodies that recognize specific short oligosaccharides with varying sulfation to evaluate the HS composition in fractones in young and aged brains.
34324645	1	26	theme	fibroblast	262:271	arg1	factor					280:285	basic fibroblast growth factor	256:285	basic fibroblast growth factor	256:285	Fractones, specialized extracellular matrix structures found in the subventricular zone (SVZ) neurogenic niche, can capture growth factors, such as basic fibroblast growth factor, from the extracellular milieu through a heparin-binding mechanism for neural stem cell (NSC) presentation, which promotes neurogenesis.
34324645	6	27	theme	mice	1090:1093	arg1	SVZ					1052:1054	the aged SVZ	1043:1054	the aged SVZ of both wild-type and Sulf1/2 DKO mice	1043:1093	While neurogenesis declined in the aged SVZ of both wild-type and Sulf1/2 DKO mice, we observed a larger number of neuroblasts in the young and aged SVZ of Sulf1/2 DKO mice.
34324645	0	28	theme	heparan	23:29	arg1	sulfate					31:37	fractone heparan sulfate	14:37	fractone heparan sulfate composition	14:49	Regulation of fractone heparan sulfate composition in young and aged subventricular zone neurogenic niches.
34324645	1	29	theme	growth	273:278	arg1	factor					280:285	basic fibroblast growth factor	256:285	basic fibroblast growth factor	256:285	Fractones, specialized extracellular matrix structures found in the subventricular zone (SVZ) neurogenic niche, can capture growth factors, such as basic fibroblast growth factor, from the extracellular milieu through a heparin-binding mechanism for neural stem cell (NSC) presentation, which promotes neurogenesis.
34324645	4	30	theme	DKO	883:885	arg1	mice					888:891	Sulf2 double knockout (DKO) mice	860:891	Sulf2 double knockout (DKO) mice	860:891	To further understand the conditions that regulate 6-O sulfation levels and its impact on neurogenesis, we used endosulfatase Sulf1 and Sulf2 double knockout (DKO) mice.
34324645	6	31	theme	DKO	1086:1088	arg1	mice					1090:1093	both wild-type and Sulf1/2 DKO mice	1059:1093	both wild-type and Sulf1/2 DKO mice	1059:1093	While neurogenesis declined in the aged SVZ of both wild-type and Sulf1/2 DKO mice, we observed a larger number of neuroblasts in the young and aged SVZ of Sulf1/2 DKO mice.
34324645	6	32	theme	aged	1156:1159	arg1	SVZ					1161:1163	the young and aged SVZ	1142:1163	the young and aged SVZ of Sulf1/2 DKO mice	1142:1183	While neurogenesis declined in the aged SVZ of both wild-type and Sulf1/2 DKO mice, we observed a larger number of neuroblasts in the young and aged SVZ of Sulf1/2 DKO mice.
34324645	0	33	theme	fractone	14:21	arg1	sulfate					31:37	fractone heparan sulfate	14:37	fractone heparan sulfate composition	14:49	Regulation of fractone heparan sulfate composition in young and aged subventricular zone neurogenic niches.
34324645	8	34	theme	current	1342:1348	arg1	understanding					1350:1362	the current understanding	1338:1362	the current understanding regarding the extracellular environment that is best suited for NSCs to thrive, which is critical for the design of future stem cell therapies	1338:1505	Our findings advance the current understanding regarding the extracellular environment that is best suited for NSCs to thrive, which is critical for the design of future stem cell therapies.
34324645	3	35	theme	short	608:612	arg1	oligosaccharides					614:629	specific short oligosaccharides	599:629	specific short oligosaccharides	599:629	In this study, we used antibodies that recognize specific short oligosaccharides with varying sulfation to evaluate the HS composition in fractones in young and aged brains.
34324645	6	36	theme	Sulf1/2	1078:1084	arg1	mice					1090:1093	both wild-type and Sulf1/2 DKO mice	1059:1093	both wild-type and Sulf1/2 DKO mice	1059:1093	While neurogenesis declined in the aged SVZ of both wild-type and Sulf1/2 DKO mice, we observed a larger number of neuroblasts in the young and aged SVZ of Sulf1/2 DKO mice.
34324645	1	37	theme	neurogenic	202:211	arg1	niche					213:217	the subventricular zone (SVZ) neurogenic niche	172:217	the subventricular zone (SVZ) neurogenic niche	172:217	Fractones, specialized extracellular matrix structures found in the subventricular zone (SVZ) neurogenic niche, can capture growth factors, such as basic fibroblast growth factor, from the extracellular milieu through a heparin-binding mechanism for neural stem cell (NSC) presentation, which promotes neurogenesis.
34324645	0	38	theme	composition	39:49	arg1	Regulation					0:9	Regulation	0:9	Regulation of fractone heparan sulfate composition in young and aged subventricular zone neurogenic niches.	0:106	Regulation of fractone heparan sulfate composition in young and aged subventricular zone neurogenic niches.
34324645	3	39	theme	aged	711:714	arg1	brains					716:721	young and aged brains	701:721	young and aged brains	701:721	In this study, we used antibodies that recognize specific short oligosaccharides with varying sulfation to evaluate the HS composition in fractones in young and aged brains.
34324645	2	40	theme	sulfate	519:525	arg1	composition					496:506	the composition	492:506	the composition of heparan sulfate (HS) within fractones	492:547	During aging, a decline in neurogenesis correlates with a change in the composition of heparan sulfate (HS) within fractones.
34324645	6	41	theme	wild-type	1064:1072	arg1	mice					1090:1093	both wild-type and Sulf1/2 DKO mice	1059:1093	both wild-type and Sulf1/2 DKO mice	1059:1093	While neurogenesis declined in the aged SVZ of both wild-type and Sulf1/2 DKO mice, we observed a larger number of neuroblasts in the young and aged SVZ of Sulf1/2 DKO mice.
34324645	0	42	dep	young	54:58	arg1	subventricular					69:82	subventricular	69:82	subventricular	69:82	Regulation of fractone heparan sulfate composition in young and aged subventricular zone neurogenic niches.
34324645	0	43	theme	sulfate	31:37	arg1	composition					39:49	fractone heparan sulfate composition	14:49	fractone heparan sulfate composition	14:49	Regulation of fractone heparan sulfate composition in young and aged subventricular zone neurogenic niches.
34324645	4	44	used	used	831:834	arg2	we					828:829	we	828:829	we	828:829	To further understand the conditions that regulate 6-O sulfation levels and its impact on neurogenesis, we used endosulfatase Sulf1 and Sulf2 double knockout (DKO) mice.
34324645	2	45	theme	heparan	511:517	arg1	HS					528:529	HS	528:529	HS	528:529	During aging, a decline in neurogenesis correlates with a change in the composition of heparan sulfate (HS) within fractones.
34324645	2	45	theme	heparan	511:517	arg1	sulfate					519:525	heparan sulfate	511:525	heparan sulfate (HS)	511:530	During aging, a decline in neurogenesis correlates with a change in the composition of heparan sulfate (HS) within fractones.
34324645	2	46	from	change	482:487	arg1	composition					496:506	the composition	492:506	the composition of heparan sulfate (HS) within fractones	492:547	During aging, a decline in neurogenesis correlates with a change in the composition of heparan sulfate (HS) within fractones.
34324645	4	47	theme	sulfation	779:787	arg1	levels					789:794	6-O sulfation levels	775:794	6-O sulfation levels	775:794	To further understand the conditions that regulate 6-O sulfation levels and its impact on neurogenesis, we used endosulfatase Sulf1 and Sulf2 double knockout (DKO) mice.
34324645	0	48	theme	young	54:58	arg1	niches					100:105	young and aged subventricular zone neurogenic niches	54:105	young and aged subventricular zone neurogenic niches	54:105	Regulation of fractone heparan sulfate composition in young and aged subventricular zone neurogenic niches.
34324645	8	49	theme	extracellular	1378:1390	arg1	environment					1392:1402	the extracellular environment	1374:1402	the extracellular environment that is best suited for NSCs to thrive, which is critical for the design of future stem cell therapies	1374:1505	Our findings advance the current understanding regarding the extracellular environment that is best suited for NSCs to thrive, which is critical for the design of future stem cell therapies.
34324645	4	50	theme	Sulf2	860:864	arg1	mice					888:891	Sulf2 double knockout (DKO) mice	860:891	Sulf2 double knockout (DKO) mice	860:891	To further understand the conditions that regulate 6-O sulfation levels and its impact on neurogenesis, we used endosulfatase Sulf1 and Sulf2 double knockout (DKO) mice.
34324645	3	51	theme	specific	599:606	arg1	oligosaccharides					614:629	specific short oligosaccharides	599:629	specific short oligosaccharides	599:629	In this study, we used antibodies that recognize specific short oligosaccharides with varying sulfation to evaluate the HS composition in fractones in young and aged brains.
34324645	6	52	theme	young	1146:1150	arg1	SVZ					1161:1163	the young and aged SVZ	1142:1163	the young and aged SVZ of Sulf1/2 DKO mice	1142:1183	While neurogenesis declined in the aged SVZ of both wild-type and Sulf1/2 DKO mice, we observed a larger number of neuroblasts in the young and aged SVZ of Sulf1/2 DKO mice.
34324645	3	53	from	composition	673:683	arg1	fractones					688:696	fractones	688:696	fractones in young and aged brains	688:721	In this study, we used antibodies that recognize specific short oligosaccharides with varying sulfation to evaluate the HS composition in fractones in young and aged brains.
34324645	1	54	theme	extracellular	297:309	arg1	milieu					311:316	the extracellular milieu	293:316	the extracellular milieu	293:316	Fractones, specialized extracellular matrix structures found in the subventricular zone (SVZ) neurogenic niche, can capture growth factors, such as basic fibroblast growth factor, from the extracellular milieu through a heparin-binding mechanism for neural stem cell (NSC) presentation, which promotes neurogenesis.
34324645	8	55	theme	future	1480:1485	arg1	therapies					1497:1505	future stem cell therapies	1480:1505	future stem cell therapies	1480:1505	Our findings advance the current understanding regarding the extracellular environment that is best suited for NSCs to thrive, which is critical for the design of future stem cell therapies.
34324645	4	56	from	levels	789:794	arg1	neurogenesis					814:825	neurogenesis	814:825	neurogenesis	814:825	To further understand the conditions that regulate 6-O sulfation levels and its impact on neurogenesis, we used endosulfatase Sulf1 and Sulf2 double knockout (DKO) mice.
34324645	1	57	theme	specialized	119:129	arg1	structures					152:161	specialized extracellular matrix structures	119:161	specialized extracellular matrix structures found in the subventricular zone (SVZ) neurogenic niche	119:217	Fractones, specialized extracellular matrix structures found in the subventricular zone (SVZ) neurogenic niche, can capture growth factors, such as basic fibroblast growth factor, from the extracellular milieu through a heparin-binding mechanism for neural stem cell (NSC) presentation, which promotes neurogenesis.
34324645	1	57	theme	specialized	119:129	arg1	Fractones					108:116	Fractones	108:116	Fractones	108:116	Fractones, specialized extracellular matrix structures found in the subventricular zone (SVZ) neurogenic niche, can capture growth factors, such as basic fibroblast growth factor, from the extracellular milieu through a heparin-binding mechanism for neural stem cell (NSC) presentation, which promotes neurogenesis.
34324645	8	58	theme	stem	1487:1490	arg1	therapies					1497:1505	future stem cell therapies	1480:1505	future stem cell therapies	1480:1505	Our findings advance the current understanding regarding the extracellular environment that is best suited for NSCs to thrive, which is critical for the design of future stem cell therapies.
34324645	4	59	theme	knockout	873:880	arg1	mice					888:891	Sulf2 double knockout (DKO) mice	860:891	Sulf2 double knockout (DKO) mice	860:891	To further understand the conditions that regulate 6-O sulfation levels and its impact on neurogenesis, we used endosulfatase Sulf1 and Sulf2 double knockout (DKO) mice.
34324645	5	60	theme	higher	990:995	arg1	sulfation					1001:1009	higher 6-O sulfation	990:1009	higher 6-O sulfation	990:1009	Fractones in the SVZ of Sulf1/2 DKO mice showed immunoreactivity for the HS epitope, suggesting higher 6-O sulfation.
34324645	5	61	theme	mice	930:933	arg1	SVZ					911:913	the SVZ	907:913	the SVZ of Sulf1/2 DKO mice	907:933	Fractones in the SVZ of Sulf1/2 DKO mice showed immunoreactivity for the HS epitope, suggesting higher 6-O sulfation.
34324645	5	62	theme	HS	967:968	arg1	epitope					970:976	the HS epitope	963:976	the HS epitope	963:976	Fractones in the SVZ of Sulf1/2 DKO mice showed immunoreactivity for the HS epitope, suggesting higher 6-O sulfation.
34324645	1	63	theme	extracellular	131:143	arg1	structures					152:161	specialized extracellular matrix structures	119:161	specialized extracellular matrix structures found in the subventricular zone (SVZ) neurogenic niche	119:217	Fractones, specialized extracellular matrix structures found in the subventricular zone (SVZ) neurogenic niche, can capture growth factors, such as basic fibroblast growth factor, from the extracellular milieu through a heparin-binding mechanism for neural stem cell (NSC) presentation, which promotes neurogenesis.
34324645	1	63	theme	extracellular	131:143	arg1	Fractones					108:116	Fractones	108:116	Fractones	108:116	Fractones, specialized extracellular matrix structures found in the subventricular zone (SVZ) neurogenic niche, can capture growth factors, such as basic fibroblast growth factor, from the extracellular milieu through a heparin-binding mechanism for neural stem cell (NSC) presentation, which promotes neurogenesis.
34324645	4	64	theme	double	866:871	arg1	mice					888:891	Sulf2 double knockout (DKO) mice	860:891	Sulf2 double knockout (DKO) mice	860:891	To further understand the conditions that regulate 6-O sulfation levels and its impact on neurogenesis, we used endosulfatase Sulf1 and Sulf2 double knockout (DKO) mice.
34324645	5	65	theme	6-O	997:999	arg1	sulfation					1001:1009	higher 6-O sulfation	990:1009	higher 6-O sulfation	990:1009	Fractones in the SVZ of Sulf1/2 DKO mice showed immunoreactivity for the HS epitope, suggesting higher 6-O sulfation.
34324645	6	66	theme	neuroblasts	1127:1137	arg1	number					1117:1122	a larger number	1108:1122	a larger number of neuroblasts	1108:1137	While neurogenesis declined in the aged SVZ of both wild-type and Sulf1/2 DKO mice, we observed a larger number of neuroblasts in the young and aged SVZ of Sulf1/2 DKO mice.
34324645	1	67	theme	matrix	145:150	arg1	structures					152:161	specialized extracellular matrix structures	119:161	specialized extracellular matrix structures found in the subventricular zone (SVZ) neurogenic niche	119:217	Fractones, specialized extracellular matrix structures found in the subventricular zone (SVZ) neurogenic niche, can capture growth factors, such as basic fibroblast growth factor, from the extracellular milieu through a heparin-binding mechanism for neural stem cell (NSC) presentation, which promotes neurogenesis.
34324645	1	67	theme	matrix	145:150	arg1	Fractones					108:116	Fractones	108:116	Fractones	108:116	Fractones, specialized extracellular matrix structures found in the subventricular zone (SVZ) neurogenic niche, can capture growth factors, such as basic fibroblast growth factor, from the extracellular milieu through a heparin-binding mechanism for neural stem cell (NSC) presentation, which promotes neurogenesis.
34324645	8	68	theme	therapies	1497:1505	arg1	design					1470:1475	the design	1466:1475	the design of future stem cell therapies	1466:1505	Our findings advance the current understanding regarding the extracellular environment that is best suited for NSCs to thrive, which is critical for the design of future stem cell therapies.
34324645	0	69	theme	aged	64:67	arg1	niches					100:105	young and aged subventricular zone neurogenic niches	54:105	young and aged subventricular zone neurogenic niches	54:105	Regulation of fractone heparan sulfate composition in young and aged subventricular zone neurogenic niches.
34324645	4	70	theme	6-O	775:777	arg1	levels					789:794	6-O sulfation levels	775:794	6-O sulfation levels	775:794	To further understand the conditions that regulate 6-O sulfation levels and its impact on neurogenesis, we used endosulfatase Sulf1 and Sulf2 double knockout (DKO) mice.
34324645	7	71	from	removal	1224:1230	arg1	HS					1262:1263	fractones HS	1252:1263	fractones HS	1252:1263	Together, these results show that the removal of 6-O-sulfation in fractones HS by endosulfatases inhibits neurogenesis in the SVZ.
34324645	3	72	used	used	568:571	arg2	we					565:566	we	565:566	we	565:566	In this study, we used antibodies that recognize specific short oligosaccharides with varying sulfation to evaluate the HS composition in fractones in young and aged brains.
34324645	5	73	from	Fractones	894:902	arg1	SVZ					911:913	the SVZ	907:913	the SVZ of Sulf1/2 DKO mice	907:933	Fractones in the SVZ of Sulf1/2 DKO mice showed immunoreactivity for the HS epitope, suggesting higher 6-O sulfation.
34324645	1	74	theme	growth	232:237	arg1	factors					239:245	growth factors	232:245	growth factors	232:245	Fractones, specialized extracellular matrix structures found in the subventricular zone (SVZ) neurogenic niche, can capture growth factors, such as basic fibroblast growth factor, from the extracellular milieu through a heparin-binding mechanism for neural stem cell (NSC) presentation, which promotes neurogenesis.
34324645	3	75	theme	HS	670:671	arg1	composition					673:683	the HS composition	666:683	the HS composition in fractones in young and aged brains	666:721	In this study, we used antibodies that recognize specific short oligosaccharides with varying sulfation to evaluate the HS composition in fractones in young and aged brains.
34324645	7	76	theme	fractones	1252:1260	arg1	HS					1262:1263	fractones HS	1252:1263	fractones HS	1252:1263	Together, these results show that the removal of 6-O-sulfation in fractones HS by endosulfatases inhibits neurogenesis in the SVZ.
34324645	6	77	theme	aged	1047:1050	arg1	SVZ					1052:1054	the aged SVZ	1043:1054	the aged SVZ of both wild-type and Sulf1/2 DKO mice	1043:1093	While neurogenesis declined in the aged SVZ of both wild-type and Sulf1/2 DKO mice, we observed a larger number of neuroblasts in the young and aged SVZ of Sulf1/2 DKO mice.
33236889	3	0	theme	sizing	564:569	arg1	agent					571:575	sizing agent	564:575	sizing agent	564:575	The well-preserved native hemicellulose as the amphiphilic shell of HCNFs could act as a binding agent, sizing agent, and even dispersing agent between HCNFs and CNTs.
33236889	2	1	theme	mild	403:406	arg1	delignification					408:422	mild delignification	403:422	mild delignification	403:422	Holocellulose nanofibrils (HCNFs) with core-shell structure were first obtained from bagasse via mild delignification and mechanical defibration process.
33236889	5	2	theme	sensing	1043:1049	arg1	field					1018:1022	the field	1014:1022	the field of flexible liquid sensing	1014:1049	Furthermore, these HCNF/CNT composites with outstanding integrated performances exhibited great potential in the field of flexible liquid sensing.
33236889	1	3	theme	multicomponent	140:153	arg1	structure					155:163	a wood-like multicomponent structure	128:163	a wood-like multicomponent structure	128:163	Inspired by a wood-like multicomponent structure, an interface-reinforced method was developed to fabricate high-performance cellulose nanofibril (CNF)/carbon nanotube (CNT) nanocomposites.
33236889	5	4	with	composites	933:942	arg1	performances					972:983	outstanding integrated performances	949:983	outstanding integrated performances	949:983	Furthermore, these HCNF/CNT composites with outstanding integrated performances exhibited great potential in the field of flexible liquid sensing.
33236889	5	5	theme	flexible	1027:1034	arg1	sensing					1043:1049	flexible liquid sensing	1027:1049	flexible liquid sensing	1027:1049	Furthermore, these HCNF/CNT composites with outstanding integrated performances exhibited great potential in the field of flexible liquid sensing.
33236889	3	6	theme	binding	549:555	arg1	agent					557:561	a binding agent	547:561	a binding agent	547:561	The well-preserved native hemicellulose as the amphiphilic shell of HCNFs could act as a binding agent, sizing agent, and even dispersing agent between HCNFs and CNTs.
33236889	3	6	theme	binding	549:555	arg1	hemicellulose					486:498	The well-preserved native hemicellulose	460:498	The well-preserved native hemicellulose as the amphiphilic shell of HCNFs	460:532	The well-preserved native hemicellulose as the amphiphilic shell of HCNFs could act as a binding agent, sizing agent, and even dispersing agent between HCNFs and CNTs.
33236889	5	7	theme	integrated	961:970	arg1	performances					972:983	outstanding integrated performances	949:983	outstanding integrated performances	949:983	Furthermore, these HCNF/CNT composites with outstanding integrated performances exhibited great potential in the field of flexible liquid sensing.
33236889	4	8	theme	CNF/CNT	880:886	arg1	films					898:902	normal CNF/CNT composite films	873:902	normal CNF/CNT composite films	873:902	Remarkably, both the tensile strength at high relative humidity (83% RH) and electrical conductivity of the HCNF/CNT nanocomposites were significantly improved up to 121 MPa and 321 S/m, respectively, demonstrating great superiority compared to normal CNF/CNT composite films.
33236889	5	9	theme	liquid	1036:1041	arg1	sensing					1043:1049	flexible liquid sensing	1027:1049	flexible liquid sensing	1027:1049	Furthermore, these HCNF/CNT composites with outstanding integrated performances exhibited great potential in the field of flexible liquid sensing.
33236889	5	10	theme	outstanding	949:959	arg1	performances					972:983	outstanding integrated performances	949:983	outstanding integrated performances	949:983	Furthermore, these HCNF/CNT composites with outstanding integrated performances exhibited great potential in the field of flexible liquid sensing.
33236889	3	11	theme	amphiphilic	507:517	arg1	shell					519:523	the amphiphilic shell	503:523	the amphiphilic shell of HCNFs	503:532	The well-preserved native hemicellulose as the amphiphilic shell of HCNFs could act as a binding agent, sizing agent, and even dispersing agent between HCNFs and CNTs.
33236889	3	12	theme	dispersing	587:596	arg1	agent					598:602	even dispersing agent	582:602	even dispersing agent	582:602	The well-preserved native hemicellulose as the amphiphilic shell of HCNFs could act as a binding agent, sizing agent, and even dispersing agent between HCNFs and CNTs.
33236889	1	13	theme	high-performance	224:239	arg1	CNF					263:265	CNF	263:265	CNF	263:265	Inspired by a wood-like multicomponent structure, an interface-reinforced method was developed to fabricate high-performance cellulose nanofibril (CNF)/carbon nanotube (CNT) nanocomposites.
33236889	1	13	theme	high-performance	224:239	arg1	nanofibril					251:260	high-performance cellulose nanofibril	224:260	high-performance cellulose nanofibril (CNF)/carbon nanotube (CNT) nanocomposites	224:303	Inspired by a wood-like multicomponent structure, an interface-reinforced method was developed to fabricate high-performance cellulose nanofibril (CNF)/carbon nanotube (CNT) nanocomposites.
33236889	0	14	theme	Bioinspired	13:23	arg1	Design					35:40	Eco-Friendly Bioinspired Interface Design	0:40	Eco-Friendly Bioinspired Interface Design for High-Performance Cellulose	0:71	Eco-Friendly Bioinspired Interface Design for High-Performance Cellulose Nanofibril/Carbon Nanotube Nanocomposites.
33236889	4	15	theme	121	794:796	arg1	MPa					798:800	121 MPa	794:800	121 MPa	794:800	Remarkably, both the tensile strength at high relative humidity (83% RH) and electrical conductivity of the HCNF/CNT nanocomposites were significantly improved up to 121 MPa and 321 S/m, respectively, demonstrating great superiority compared to normal CNF/CNT composite films.
33236889	1	16	theme	cellulose	241:249	arg1	CNF					263:265	CNF	263:265	CNF	263:265	Inspired by a wood-like multicomponent structure, an interface-reinforced method was developed to fabricate high-performance cellulose nanofibril (CNF)/carbon nanotube (CNT) nanocomposites.
33236889	1	16	theme	cellulose	241:249	arg1	nanofibril					251:260	high-performance cellulose nanofibril	224:260	high-performance cellulose nanofibril (CNF)/carbon nanotube (CNT) nanocomposites	224:303	Inspired by a wood-like multicomponent structure, an interface-reinforced method was developed to fabricate high-performance cellulose nanofibril (CNF)/carbon nanotube (CNT) nanocomposites.
33236889	0	17	theme	Eco-Friendly	0:11	arg1	Design					35:40	Eco-Friendly Bioinspired Interface Design	0:40	Eco-Friendly Bioinspired Interface Design for High-Performance Cellulose	0:71	Eco-Friendly Bioinspired Interface Design for High-Performance Cellulose Nanofibril/Carbon Nanotube Nanocomposites.
33236889	4	18	theme	composite	888:896	arg1	films					898:902	normal CNF/CNT composite films	873:902	normal CNF/CNT composite films	873:902	Remarkably, both the tensile strength at high relative humidity (83% RH) and electrical conductivity of the HCNF/CNT nanocomposites were significantly improved up to 121 MPa and 321 S/m, respectively, demonstrating great superiority compared to normal CNF/CNT composite films.
33236889	2	19	theme	defibration	439:449	arg1	process					451:457	mechanical defibration process	428:457	mechanical defibration process	428:457	Holocellulose nanofibrils (HCNFs) with core-shell structure were first obtained from bagasse via mild delignification and mechanical defibration process.
33236889	5	20	theme	great	995:999	arg1	potential					1001:1009	great potential	995:1009	great potential	995:1009	Furthermore, these HCNF/CNT composites with outstanding integrated performances exhibited great potential in the field of flexible liquid sensing.
33236889	1	21	theme	wood-like	130:138	arg1	structure					155:163	a wood-like multicomponent structure	128:163	a wood-like multicomponent structure	128:163	Inspired by a wood-like multicomponent structure, an interface-reinforced method was developed to fabricate high-performance cellulose nanofibril (CNF)/carbon nanotube (CNT) nanocomposites.
33236889	0	22	theme	Interface	25:33	arg1	Design					35:40	Eco-Friendly Bioinspired Interface Design	0:40	Eco-Friendly Bioinspired Interface Design for High-Performance Cellulose	0:71	Eco-Friendly Bioinspired Interface Design for High-Performance Cellulose Nanofibril/Carbon Nanotube Nanocomposites.
33236889	2	23	theme	core-shell	345:354	arg1	structure					356:364	core-shell structure	345:364	core-shell structure	345:364	Holocellulose nanofibrils (HCNFs) with core-shell structure were first obtained from bagasse via mild delignification and mechanical defibration process.
33236889	4	24	from	humidity	683:690	arg1	strength					657:664	the tensile strength	645:664	the tensile strength at high relative humidity (83% RH)	645:699	Remarkably, both the tensile strength at high relative humidity (83% RH) and electrical conductivity of the HCNF/CNT nanocomposites were significantly improved up to 121 MPa and 321 S/m, respectively, demonstrating great superiority compared to normal CNF/CNT composite films.
33236889	4	24	from	humidity	683:690	arg1	conductivity					716:727	electrical conductivity	705:727	electrical conductivity of the HCNF/CNT nanocomposites	705:758	Remarkably, both the tensile strength at high relative humidity (83% RH) and electrical conductivity of the HCNF/CNT nanocomposites were significantly improved up to 121 MPa and 321 S/m, respectively, demonstrating great superiority compared to normal CNF/CNT composite films.
33236889	4	25	theme	relative	674:681	arg1	RH					697:698	83% RH	693:698	83% RH	693:698	Remarkably, both the tensile strength at high relative humidity (83% RH) and electrical conductivity of the HCNF/CNT nanocomposites were significantly improved up to 121 MPa and 321 S/m, respectively, demonstrating great superiority compared to normal CNF/CNT composite films.
33236889	4	25	theme	relative	674:681	arg1	humidity					683:690	high relative humidity	669:690	high relative humidity (83% RH)	669:699	Remarkably, both the tensile strength at high relative humidity (83% RH) and electrical conductivity of the HCNF/CNT nanocomposites were significantly improved up to 121 MPa and 321 S/m, respectively, demonstrating great superiority compared to normal CNF/CNT composite films.
33236889	0	26	theme	High-Performance	46:61	arg1	Cellulose					63:71	High-Performance Cellulose	46:71	High-Performance Cellulose	46:71	Eco-Friendly Bioinspired Interface Design for High-Performance Cellulose Nanofibril/Carbon Nanotube Nanocomposites.
33236889	4	27	theme	83	693:694	arg1	%					695:695	%	695:695	%	695:695	Remarkably, both the tensile strength at high relative humidity (83% RH) and electrical conductivity of the HCNF/CNT nanocomposites were significantly improved up to 121 MPa and 321 S/m, respectively, demonstrating great superiority compared to normal CNF/CNT composite films.
33236889	1	28	theme	nanofibril	251:260	arg1	nanocomposites					290:303	high-performance cellulose nanofibril (CNF)/carbon nanotube (CNT) nanocomposites	224:303	high-performance cellulose nanofibril (CNF)/carbon nanotube (CNT) nanocomposites	224:303	Inspired by a wood-like multicomponent structure, an interface-reinforced method was developed to fabricate high-performance cellulose nanofibril (CNF)/carbon nanotube (CNT) nanocomposites.
33236889	3	29	theme	HCNFs	528:532	arg1	shell					519:523	the amphiphilic shell	503:523	the amphiphilic shell of HCNFs	503:532	The well-preserved native hemicellulose as the amphiphilic shell of HCNFs could act as a binding agent, sizing agent, and even dispersing agent between HCNFs and CNTs.
33236889	4	30	theme	great	843:847	arg1	superiority					849:859	great superiority	843:859	great superiority	843:859	Remarkably, both the tensile strength at high relative humidity (83% RH) and electrical conductivity of the HCNF/CNT nanocomposites were significantly improved up to 121 MPa and 321 S/m, respectively, demonstrating great superiority compared to normal CNF/CNT composite films.
33236889	1	31	theme	/carbon	267:273	arg1	nanocomposites					290:303	high-performance cellulose nanofibril (CNF)/carbon nanotube (CNT) nanocomposites	224:303	high-performance cellulose nanofibril (CNF)/carbon nanotube (CNT) nanocomposites	224:303	Inspired by a wood-like multicomponent structure, an interface-reinforced method was developed to fabricate high-performance cellulose nanofibril (CNF)/carbon nanotube (CNT) nanocomposites.
33236889	3	32	theme	well-preserved	464:477	arg1	agent					557:561	a binding agent	547:561	a binding agent	547:561	The well-preserved native hemicellulose as the amphiphilic shell of HCNFs could act as a binding agent, sizing agent, and even dispersing agent between HCNFs and CNTs.
33236889	3	32	theme	well-preserved	464:477	arg1	hemicellulose					486:498	The well-preserved native hemicellulose	460:498	The well-preserved native hemicellulose as the amphiphilic shell of HCNFs	460:532	The well-preserved native hemicellulose as the amphiphilic shell of HCNFs could act as a binding agent, sizing agent, and even dispersing agent between HCNFs and CNTs.
33236889	5	33	theme	HCNF/CNT	924:931	arg1	composites					933:942	these HCNF/CNT composites	918:942	these HCNF/CNT composites with outstanding integrated performances	918:983	Furthermore, these HCNF/CNT composites with outstanding integrated performances exhibited great potential in the field of flexible liquid sensing.
33236889	4	34	theme	nanocomposites	745:758	arg1	strength					657:664	the tensile strength	645:664	the tensile strength at high relative humidity (83% RH)	645:699	Remarkably, both the tensile strength at high relative humidity (83% RH) and electrical conductivity of the HCNF/CNT nanocomposites were significantly improved up to 121 MPa and 321 S/m, respectively, demonstrating great superiority compared to normal CNF/CNT composite films.
33236889	4	34	theme	nanocomposites	745:758	arg1	conductivity					716:727	electrical conductivity	705:727	electrical conductivity of the HCNF/CNT nanocomposites	705:758	Remarkably, both the tensile strength at high relative humidity (83% RH) and electrical conductivity of the HCNF/CNT nanocomposites were significantly improved up to 121 MPa and 321 S/m, respectively, demonstrating great superiority compared to normal CNF/CNT composite films.
33236889	3	35	theme	native	479:484	arg1	agent					557:561	a binding agent	547:561	a binding agent	547:561	The well-preserved native hemicellulose as the amphiphilic shell of HCNFs could act as a binding agent, sizing agent, and even dispersing agent between HCNFs and CNTs.
33236889	3	35	theme	native	479:484	arg1	hemicellulose					486:498	The well-preserved native hemicellulose	460:498	The well-preserved native hemicellulose as the amphiphilic shell of HCNFs	460:532	The well-preserved native hemicellulose as the amphiphilic shell of HCNFs could act as a binding agent, sizing agent, and even dispersing agent between HCNFs and CNTs.
33236889	2	36	with	nanofibrils	320:330	arg1	structure					356:364	core-shell structure	345:364	core-shell structure	345:364	Holocellulose nanofibrils (HCNFs) with core-shell structure were first obtained from bagasse via mild delignification and mechanical defibration process.
33236889	4	37	theme	normal	873:878	arg1	films					898:902	normal CNF/CNT composite films	873:902	normal CNF/CNT composite films	873:902	Remarkably, both the tensile strength at high relative humidity (83% RH) and electrical conductivity of the HCNF/CNT nanocomposites were significantly improved up to 121 MPa and 321 S/m, respectively, demonstrating great superiority compared to normal CNF/CNT composite films.
33236889	4	38	theme	HCNF/CNT	736:743	arg1	nanocomposites					745:758	the HCNF/CNT nanocomposites	732:758	the HCNF/CNT nanocomposites	732:758	Remarkably, both the tensile strength at high relative humidity (83% RH) and electrical conductivity of the HCNF/CNT nanocomposites were significantly improved up to 121 MPa and 321 S/m, respectively, demonstrating great superiority compared to normal CNF/CNT composite films.
33236889	4	39	dep	MPa	798:800	arg1	up					788:789	up	788:789	up	788:789	Remarkably, both the tensile strength at high relative humidity (83% RH) and electrical conductivity of the HCNF/CNT nanocomposites were significantly improved up to 121 MPa and 321 S/m, respectively, demonstrating great superiority compared to normal CNF/CNT composite films.
33236889	0	40	dep	Nanocomposites	100:113	arg1	Design					35:40	Eco-Friendly Bioinspired Interface Design	0:40	Eco-Friendly Bioinspired Interface Design for High-Performance Cellulose	0:71	Eco-Friendly Bioinspired Interface Design for High-Performance Cellulose Nanofibril/Carbon Nanotube Nanocomposites.
33236889	1	41	theme	nanotube	275:282	arg1	nanocomposites					290:303	high-performance cellulose nanofibril (CNF)/carbon nanotube (CNT) nanocomposites	224:303	high-performance cellulose nanofibril (CNF)/carbon nanotube (CNT) nanocomposites	224:303	Inspired by a wood-like multicomponent structure, an interface-reinforced method was developed to fabricate high-performance cellulose nanofibril (CNF)/carbon nanotube (CNT) nanocomposites.
33236889	4	42	theme	321	806:808	arg1	S/m					810:812	321 S/m	806:812	321 S/m	806:812	Remarkably, both the tensile strength at high relative humidity (83% RH) and electrical conductivity of the HCNF/CNT nanocomposites were significantly improved up to 121 MPa and 321 S/m, respectively, demonstrating great superiority compared to normal CNF/CNT composite films.
33236889	2	43	theme	Holocellulose	306:318	arg1	HCNFs					333:337	HCNFs	333:337	HCNFs	333:337	Holocellulose nanofibrils (HCNFs) with core-shell structure were first obtained from bagasse via mild delignification and mechanical defibration process.
33236889	2	43	theme	Holocellulose	306:318	arg1	nanofibrils					320:330	Holocellulose nanofibrils	306:330	Holocellulose nanofibrils (HCNFs) with core-shell structure	306:364	Holocellulose nanofibrils (HCNFs) with core-shell structure were first obtained from bagasse via mild delignification and mechanical defibration process.
33236889	1	44	theme	interface-reinforced	169:188	arg1	method					190:195	an interface-reinforced method	166:195	an interface-reinforced method	166:195	Inspired by a wood-like multicomponent structure, an interface-reinforced method was developed to fabricate high-performance cellulose nanofibril (CNF)/carbon nanotube (CNT) nanocomposites.
33236889	4	45	theme	tensile	649:655	arg1	strength					657:664	the tensile strength	645:664	the tensile strength at high relative humidity (83% RH)	645:699	Remarkably, both the tensile strength at high relative humidity (83% RH) and electrical conductivity of the HCNF/CNT nanocomposites were significantly improved up to 121 MPa and 321 S/m, respectively, demonstrating great superiority compared to normal CNF/CNT composite films.
33236889	4	46	theme	high	669:672	arg1	RH					697:698	83% RH	693:698	83% RH	693:698	Remarkably, both the tensile strength at high relative humidity (83% RH) and electrical conductivity of the HCNF/CNT nanocomposites were significantly improved up to 121 MPa and 321 S/m, respectively, demonstrating great superiority compared to normal CNF/CNT composite films.
33236889	4	46	theme	high	669:672	arg1	humidity					683:690	high relative humidity	669:690	high relative humidity (83% RH)	669:699	Remarkably, both the tensile strength at high relative humidity (83% RH) and electrical conductivity of the HCNF/CNT nanocomposites were significantly improved up to 121 MPa and 321 S/m, respectively, demonstrating great superiority compared to normal CNF/CNT composite films.
33236889	2	47	theme	mechanical	428:437	arg1	process					451:457	mechanical defibration process	428:457	mechanical defibration process	428:457	Holocellulose nanofibrils (HCNFs) with core-shell structure were first obtained from bagasse via mild delignification and mechanical defibration process.
33236889	4	48	theme	%	695:695	arg1	RH					697:698	83% RH	693:698	83% RH	693:698	Remarkably, both the tensile strength at high relative humidity (83% RH) and electrical conductivity of the HCNF/CNT nanocomposites were significantly improved up to 121 MPa and 321 S/m, respectively, demonstrating great superiority compared to normal CNF/CNT composite films.
33236889	4	48	theme	%	695:695	arg1	humidity					683:690	high relative humidity	669:690	high relative humidity (83% RH)	669:699	Remarkably, both the tensile strength at high relative humidity (83% RH) and electrical conductivity of the HCNF/CNT nanocomposites were significantly improved up to 121 MPa and 321 S/m, respectively, demonstrating great superiority compared to normal CNF/CNT composite films.
33236889	1	49	theme	CNT	285:287	arg1	nanocomposites					290:303	high-performance cellulose nanofibril (CNF)/carbon nanotube (CNT) nanocomposites	224:303	high-performance cellulose nanofibril (CNF)/carbon nanotube (CNT) nanocomposites	224:303	Inspired by a wood-like multicomponent structure, an interface-reinforced method was developed to fabricate high-performance cellulose nanofibril (CNF)/carbon nanotube (CNT) nanocomposites.
33236889	4	50	theme	electrical	705:714	arg1	conductivity					716:727	electrical conductivity	705:727	electrical conductivity of the HCNF/CNT nanocomposites	705:758	Remarkably, both the tensile strength at high relative humidity (83% RH) and electrical conductivity of the HCNF/CNT nanocomposites were significantly improved up to 121 MPa and 321 S/m, respectively, demonstrating great superiority compared to normal CNF/CNT composite films.
32526930	11	0	theme	sociocultural	1551:1563	arg1	factors					1583:1589	sociocultural and environmental factors	1551:1589	sociocultural and environmental factors	1551:1589	Targeted genomic analyses may help in determining whether these differences are due to genetic variations or to sociocultural and environmental factors.
32526930	1	1	theme	breast	143:148	arg1	milk					150:153	human breast milk	137:153	human breast milk	137:153	The composition of human breast milk is affected by several factors, including genetics, geographic location and maternal nutrition.
32526930	1	2	theme	maternal	231:238	arg1	nutrition					240:248	maternal nutrition	231:248	maternal nutrition	231:248	The composition of human breast milk is affected by several factors, including genetics, geographic location and maternal nutrition.
32526930	0	3	theme	Milk	67:70	arg1	Microbiota					72:81	the Milk Microbiota	63:81	the Milk Microbiota of Breastfeeding Mothers in Dubai	63:115	Profiles of Human Milk Oligosaccharides and Their Relations to the Milk Microbiota of Breastfeeding Mothers in Dubai.
32526930	8	4	dep	detected	1051:1058	arg1	compared					1082:1089	compared	1082:1089	compared to mothers from other countries	1082:1121	Bifidobacterium and Lactobacillus were considerably lower in Dubai-based mothers, while Pseudomonas and Delftia (Hydrogenophaga) were detected at a higher abundance compared to mothers from other countries.
32526930	5	5	theme	16S	642:644	arg1	gene					651:654	the 16S rRNA gene	638:654	the 16S rRNA gene	638:654	Microbiota profiles were determined by sequencing amplicons of the V3-V4 region of the 16S rRNA gene.
32526930	1	6	theme	milk	150:153	arg1	composition					122:132	The composition	118:132	The composition of human breast milk	118:153	The composition of human breast milk is affected by several factors, including genetics, geographic location and maternal nutrition.
32526930	11	7	theme	Targeted	1439:1446	arg1	analyses					1456:1463	Targeted genomic analyses	1439:1463	Targeted genomic analyses	1439:1463	Targeted genomic analyses may help in determining whether these differences are due to genetic variations or to sociocultural and environmental factors.
32526930	0	8	theme	Breastfeeding	86:98	arg1	Mothers					100:106	Breastfeeding Mothers	86:106	Breastfeeding Mothers in Dubai	86:115	Profiles of Human Milk Oligosaccharides and Their Relations to the Milk Microbiota of Breastfeeding Mothers in Dubai.
32526930	5	9	theme	rRNA	646:649	arg1	gene					651:654	the 16S rRNA gene	638:654	the 16S rRNA gene	638:654	Microbiota profiles were determined by sequencing amplicons of the V3-V4 region of the 16S rRNA gene.
32526930	5	10	theme	sequencing	594:603	arg1	amplicons					605:613	sequencing amplicons	594:613	sequencing amplicons of the V3-V4 region of the 16S rRNA gene	594:654	Microbiota profiles were determined by sequencing amplicons of the V3-V4 region of the 16S rRNA gene.
32526930	2	11	theme	human	279:283	arg1	oligosaccharides					290:305	the human milk oligosaccharides	275:305	the human milk oligosaccharides (HMOs) of breastfeeding mothers living in Dubai and their relations with the milk microbiota	275:398	This study investigated the human milk oligosaccharides (HMOs) of breastfeeding mothers living in Dubai and their relations with the milk microbiota.
32526930	2	11	theme	human	279:283	arg1	HMOs					308:311	HMOs	308:311	HMOs	308:311	This study investigated the human milk oligosaccharides (HMOs) of breastfeeding mothers living in Dubai and their relations with the milk microbiota.
32526930	11	12	theme	genomic	1448:1454	arg1	analyses					1456:1463	Targeted genomic analyses	1439:1463	Targeted genomic analyses	1439:1463	Targeted genomic analyses may help in determining whether these differences are due to genetic variations or to sociocultural and environmental factors.
32526930	5	13	theme	gene	651:654	arg1	region					628:633	the V3-V4 region	618:633	the V3-V4 region of the 16S rRNA gene	618:654	Microbiota profiles were determined by sequencing amplicons of the V3-V4 region of the 16S rRNA gene.
32526930	7	14	dep	-secretor	886:894	arg1	Le-Se+					897:902	Le-Se+	897:902	Le-Se+	897:902	The Le (a-b+)-secretor (Le+Se+) type was the most abundant in Dubai mothers (60%), followed by the Le(a-b-)-secretor (Le-Se+) type (23%).
32526930	7	15	from	abundant	829:836	arg1	mothers					847:853	Dubai mothers	841:853	Dubai mothers (60%)	841:859	The Le (a-b+)-secretor (Le+Se+) type was the most abundant in Dubai mothers (60%), followed by the Le(a-b-)-secretor (Le-Se+) type (23%).
32526930	7	15	from	abundant	829:836	arg1	%					858:858	60%	856:858	60%	856:858	The Le (a-b+)-secretor (Le+Se+) type was the most abundant in Dubai mothers (60%), followed by the Le(a-b-)-secretor (Le-Se+) type (23%).
32526930	0	16	theme	Mothers	100:106	arg1	Microbiota					72:81	the Milk Microbiota	63:81	the Milk Microbiota of Breastfeeding Mothers in Dubai	63:115	Profiles of Human Milk Oligosaccharides and Their Relations to the Milk Microbiota of Breastfeeding Mothers in Dubai.
32526930	5	17	theme	V3-V4	622:626	arg1	region					628:633	the V3-V4 region	618:633	the V3-V4 region of the 16S rRNA gene	618:654	Microbiota profiles were determined by sequencing amplicons of the V3-V4 region of the 16S rRNA gene.
32526930	5	18	theme	Microbiota	555:564	arg1	profiles					566:573	Microbiota profiles	555:573	Microbiota profiles	555:573	Microbiota profiles were determined by sequencing amplicons of the V3-V4 region of the 16S rRNA gene.
32526930	3	19	theme	breast	415:420	arg1	samples					427:433	30 breast milk samples	412:433	30 breast milk samples	412:433	A total of 30 breast milk samples were collected from healthy Emirati and UAE-expatriates at Latifa Hospital.
32526930	8	20	theme	higher	1065:1070	arg1	abundance					1072:1080	a higher abundance	1063:1080	a higher abundance	1063:1080	Bifidobacterium and Lactobacillus were considerably lower in Dubai-based mothers, while Pseudomonas and Delftia (Hydrogenophaga) were detected at a higher abundance compared to mothers from other countries.
32526930	10	21	theme	breastfeeding	1346:1358	arg1	mothers					1360:1366	breastfeeding mothers	1346:1366	breastfeeding mothers in Dubai	1346:1375	The study highlights the HMO profiles of breastfeeding mothers in Dubai and reveals few correlations with milk microbial composition.
32526930	5	22	theme	region	628:633	arg1	amplicons					605:613	sequencing amplicons	594:613	sequencing amplicons of the V3-V4 region of the 16S rRNA gene	594:654	Microbiota profiles were determined by sequencing amplicons of the V3-V4 region of the 16S rRNA gene.
32526930	10	23	theme	mothers	1360:1366	arg1	profiles					1334:1341	the HMO profiles	1326:1341	the HMO profiles of breastfeeding mothers in Dubai	1326:1375	The study highlights the HMO profiles of breastfeeding mothers in Dubai and reveals few correlations with milk microbial composition.
32526930	10	24	theme	microbial	1416:1424	arg1	composition					1426:1436	milk microbial composition	1411:1436	milk microbial composition	1411:1436	The study highlights the HMO profiles of breastfeeding mothers in Dubai and reveals few correlations with milk microbial composition.
32526930	7	25	dep	abundant	829:836	arg1	abundant					829:836	abundant	829:836	abundant	829:836	The Le (a-b+)-secretor (Le+Se+) type was the most abundant in Dubai mothers (60%), followed by the Le(a-b-)-secretor (Le-Se+) type (23%).
32526930	7	25	dep	abundant	829:836	arg1	followed					862:869	followed	862:869	followed by the Le(a-b-)-secretor (Le-Se+) type (23%)	862:914	The Le (a-b+)-secretor (Le+Se+) type was the most abundant in Dubai mothers (60%), followed by the Le(a-b-)-secretor (Le-Se+) type (23%).
32526930	7	25	dep	abundant	829:836	arg1	type					811:814	The Le (a-b+)-secretor (Le+Se+) type	779:814	The Le (a-b+)-secretor (Le+Se+) type	779:814	The Le (a-b+)-secretor (Le+Se+) type was the most abundant in Dubai mothers (60%), followed by the Le(a-b-)-secretor (Le-Se+) type (23%).
32526930	7	26	from	mothers	847:853	arg1	abundant					829:836	abundant	829:836	abundant	829:836	The Le (a-b+)-secretor (Le+Se+) type was the most abundant in Dubai mothers (60%), followed by the Le(a-b-)-secretor (Le-Se+) type (23%).
32526930	7	26	from	mothers	847:853	arg1	followed					862:869	followed	862:869	followed by the Le(a-b-)-secretor (Le-Se+) type (23%)	862:914	The Le (a-b+)-secretor (Le+Se+) type was the most abundant in Dubai mothers (60%), followed by the Le(a-b-)-secretor (Le-Se+) type (23%).
32526930	7	26	from	mothers	847:853	arg1	type					811:814	The Le (a-b+)-secretor (Le+Se+) type	779:814	The Le (a-b+)-secretor (Le+Se+) type	779:814	The Le (a-b+)-secretor (Le+Se+) type was the most abundant in Dubai mothers (60%), followed by the Le(a-b-)-secretor (Le-Se+) type (23%).
32526930	7	27	theme	-secretor	792:800	arg1	abundant					829:836	abundant	829:836	abundant	829:836	The Le (a-b+)-secretor (Le+Se+) type was the most abundant in Dubai mothers (60%), followed by the Le(a-b-)-secretor (Le-Se+) type (23%).
32526930	7	27	theme	-secretor	792:800	arg1	followed					862:869	followed	862:869	followed by the Le(a-b-)-secretor (Le-Se+) type (23%)	862:914	The Le (a-b+)-secretor (Le+Se+) type was the most abundant in Dubai mothers (60%), followed by the Le(a-b-)-secretor (Le-Se+) type (23%).
32526930	7	27	theme	-secretor	792:800	arg1	type					811:814	The Le (a-b+)-secretor (Le+Se+) type	779:814	The Le (a-b+)-secretor (Le+Se+) type	779:814	The Le (a-b+)-secretor (Le+Se+) type was the most abundant in Dubai mothers (60%), followed by the Le(a-b-)-secretor (Le-Se+) type (23%).
32526930	1	28	theme	several	170:176	arg1	location					218:225	geographic location	207:225	geographic location	207:225	The composition of human breast milk is affected by several factors, including genetics, geographic location and maternal nutrition.
32526930	1	28	theme	several	170:176	arg1	nutrition					240:248	maternal nutrition	231:248	maternal nutrition	231:248	The composition of human breast milk is affected by several factors, including genetics, geographic location and maternal nutrition.
32526930	1	28	theme	several	170:176	arg1	factors					178:184	several factors	170:184	several factors	170:184	The composition of human breast milk is affected by several factors, including genetics, geographic location and maternal nutrition.
32526930	1	28	theme	several	170:176	arg1	genetics					197:204	genetics	197:204	genetics	197:204	The composition of human breast milk is affected by several factors, including genetics, geographic location and maternal nutrition.
32526930	0	29	theme	Milk	18:21	arg1	Oligosaccharides					23:38	Human Milk Oligosaccharides	12:38	Human Milk Oligosaccharides	12:38	Profiles of Human Milk Oligosaccharides and Their Relations to the Milk Microbiota of Breastfeeding Mothers in Dubai.
32526930	10	30	from	mothers	1360:1366	arg1	Dubai					1371:1375	Dubai	1371:1375	Dubai	1371:1375	The study highlights the HMO profiles of breastfeeding mothers in Dubai and reveals few correlations with milk microbial composition.
32526930	10	31	theme	HMO	1330:1332	arg1	profiles					1334:1341	the HMO profiles	1326:1341	the HMO profiles of breastfeeding mothers in Dubai	1326:1375	The study highlights the HMO profiles of breastfeeding mothers in Dubai and reveals few correlations with milk microbial composition.
32526930	8	32	located	detected	1051:1058	arg1	abundance					1072:1080	a higher abundance	1063:1080	a higher abundance	1063:1080	Bifidobacterium and Lactobacillus were considerably lower in Dubai-based mothers, while Pseudomonas and Delftia (Hydrogenophaga) were detected at a higher abundance compared to mothers from other countries.
32526930	8	32	located	detected	1051:1058	arg2	Pseudomonas					1005:1015	Pseudomonas and Delftia (Hydrogenophaga)	1005:1044	Pseudomonas	1005:1015	Bifidobacterium and Lactobacillus were considerably lower in Dubai-based mothers, while Pseudomonas and Delftia (Hydrogenophaga) were detected at a higher abundance compared to mothers from other countries.
32526930	8	32	located	detected	1051:1058	arg2	Hydrogenophaga					1030:1043	Hydrogenophaga	1030:1043	Hydrogenophaga	1030:1043	Bifidobacterium and Lactobacillus were considerably lower in Dubai-based mothers, while Pseudomonas and Delftia (Hydrogenophaga) were detected at a higher abundance compared to mothers from other countries.
32526930	8	32	located	detected	1051:1058	arg2	Delftia					1021:1027	Delftia	1021:1027	Delftia (Hydrogenophaga)	1021:1044	Bifidobacterium and Lactobacillus were considerably lower in Dubai-based mothers, while Pseudomonas and Delftia (Hydrogenophaga) were detected at a higher abundance compared to mothers from other countries.
32526930	0	33	theme	Human	12:16	arg1	Oligosaccharides					23:38	Human Milk Oligosaccharides	12:38	Human Milk Oligosaccharides	12:38	Profiles of Human Milk Oligosaccharides and Their Relations to the Milk Microbiota of Breastfeeding Mothers in Dubai.
32526930	0	34	from	Microbiota	72:81	arg1	Dubai					111:115	Dubai	111:115	Dubai	111:115	Profiles of Human Milk Oligosaccharides and Their Relations to the Milk Microbiota of Breastfeeding Mothers in Dubai.
32526930	2	35	theme	milk	285:288	arg1	oligosaccharides					290:305	the human milk oligosaccharides	275:305	the human milk oligosaccharides (HMOs) of breastfeeding mothers living in Dubai and their relations with the milk microbiota	275:398	This study investigated the human milk oligosaccharides (HMOs) of breastfeeding mothers living in Dubai and their relations with the milk microbiota.
32526930	2	35	theme	milk	285:288	arg1	HMOs					308:311	HMOs	308:311	HMOs	308:311	This study investigated the human milk oligosaccharides (HMOs) of breastfeeding mothers living in Dubai and their relations with the milk microbiota.
32526930	6	36	theme	HMO	657:659	arg1	concentrations					661:674	HMO concentrations	657:674	HMO concentrations	657:674	HMO concentrations were significantly higher in Emirati, and dropped with the lactation period in both groups of mothers.
32526930	10	37	from	Dubai	1371:1375	arg1	profiles					1334:1341	the HMO profiles	1326:1341	the HMO profiles of breastfeeding mothers in Dubai	1326:1375	The study highlights the HMO profiles of breastfeeding mothers in Dubai and reveals few correlations with milk microbial composition.
32526930	8	38	theme	Dubai-based	978:988	arg1	mothers					990:996	Dubai-based mothers	978:996	Dubai-based mothers	978:996	Bifidobacterium and Lactobacillus were considerably lower in Dubai-based mothers, while Pseudomonas and Delftia (Hydrogenophaga) were detected at a higher abundance compared to mothers from other countries.
32526930	10	39	with	correlations	1393:1404	arg1	composition					1426:1436	milk microbial composition	1411:1436	milk microbial composition	1411:1436	The study highlights the HMO profiles of breastfeeding mothers in Dubai and reveals few correlations with milk microbial composition.
32526930	3	40	theme	milk	422:425	arg1	samples					427:433	30 breast milk samples	412:433	30 breast milk samples	412:433	A total of 30 breast milk samples were collected from healthy Emirati and UAE-expatriates at Latifa Hospital.
32526930	0	41	theme	Oligosaccharides	23:38	arg1	Profiles					0:7	Profiles	0:7	Profiles of Human Milk Oligosaccharides	0:38	Profiles of Human Milk Oligosaccharides and Their Relations to the Milk Microbiota of Breastfeeding Mothers in Dubai.
32526930	0	41	theme	Oligosaccharides	23:38	arg1	Relations					50:58	Their Relations	44:58	Their Relations to the Milk Microbiota of Breastfeeding Mothers in Dubai	44:115	Profiles of Human Milk Oligosaccharides and Their Relations to the Milk Microbiota of Breastfeeding Mothers in Dubai.
32526930	4	42	theme	HMO	511:513	arg1	profiling					515:523	HMO profiling	511:523	HMO profiling	511:523	HMO profiling was performed using UHPLC-MS.
32526930	10	43	theme	milk	1411:1414	arg1	composition					1426:1436	milk microbial composition	1411:1436	milk microbial composition	1411:1436	The study highlights the HMO profiles of breastfeeding mothers in Dubai and reveals few correlations with milk microbial composition.
32526930	2	44	theme	milk	384:387	arg1	microbiota					389:398	the milk microbiota	380:398	the milk microbiota	380:398	This study investigated the human milk oligosaccharides (HMOs) of breastfeeding mothers living in Dubai and their relations with the milk microbiota.
32526930	8	45	theme	other	1107:1111	arg1	countries					1113:1121	other countries	1107:1121	other countries	1107:1121	Bifidobacterium and Lactobacillus were considerably lower in Dubai-based mothers, while Pseudomonas and Delftia (Hydrogenophaga) were detected at a higher abundance compared to mothers from other countries.
32526930	3	46	theme	samples	427:433	arg1	total					403:407	A total	401:407	A total of 30 breast milk samples	401:433	A total of 30 breast milk samples were collected from healthy Emirati and UAE-expatriates at Latifa Hospital.
32526930	2	47	theme	mothers	331:337	arg1	oligosaccharides					290:305	the human milk oligosaccharides	275:305	the human milk oligosaccharides (HMOs) of breastfeeding mothers living in Dubai and their relations with the milk microbiota	275:398	This study investigated the human milk oligosaccharides (HMOs) of breastfeeding mothers living in Dubai and their relations with the milk microbiota.
32526930	2	47	theme	mothers	331:337	arg1	HMOs					308:311	HMOs	308:311	HMOs	308:311	This study investigated the human milk oligosaccharides (HMOs) of breastfeeding mothers living in Dubai and their relations with the milk microbiota.
32526930	6	48	theme	lactation	735:743	arg1	period					745:750	the lactation period	731:750	the lactation period	731:750	HMO concentrations were significantly higher in Emirati, and dropped with the lactation period in both groups of mothers.
32526930	2	49	theme	breastfeeding	317:329	arg1	mothers					331:337	breastfeeding mothers	317:337	breastfeeding mothers living in Dubai and their relations with the milk microbiota	317:398	This study investigated the human milk oligosaccharides (HMOs) of breastfeeding mothers living in Dubai and their relations with the milk microbiota.
32526930	10	50	theme	few	1389:1391	arg1	correlations					1393:1404	few correlations	1389:1404	few correlations with milk microbial composition	1389:1436	The study highlights the HMO profiles of breastfeeding mothers in Dubai and reveals few correlations with milk microbial composition.
32526930	0	51	from	Mothers	100:106	arg1	Dubai					111:115	Dubai	111:115	Dubai	111:115	Profiles of Human Milk Oligosaccharides and Their Relations to the Milk Microbiota of Breastfeeding Mothers in Dubai.
32526930	8	52	from	countries	1113:1121	arg1	mothers					1094:1100	mothers	1094:1100	mothers from other countries	1094:1121	Bifidobacterium and Lactobacillus were considerably lower in Dubai-based mothers, while Pseudomonas and Delftia (Hydrogenophaga) were detected at a higher abundance compared to mothers from other countries.
32526930	6	53	theme	mothers	770:776	arg1	mothers					770:776	mothers	770:776	mothers	770:776	HMO concentrations were significantly higher in Emirati, and dropped with the lactation period in both groups of mothers.
32526930	6	53	theme	mothers	770:776	arg1	groups					760:765	both groups	755:765	both groups of mothers	755:776	HMO concentrations were significantly higher in Emirati, and dropped with the lactation period in both groups of mothers.
32526930	11	54	theme	genetic	1526:1532	arg1	variations					1534:1543	genetic variations	1526:1543	genetic variations	1526:1543	Targeted genomic analyses may help in determining whether these differences are due to genetic variations or to sociocultural and environmental factors.
32526930	9	55	theme	sialyl-lacto-N-tetraose	1154:1176	arg1	c					1178:1178	sialyl-lacto-N-tetraose c	1154:1178	sialyl-lacto-N-tetraose c	1154:1178	Atopobium was correlated with sialyl-lacto-N-tetraose c, Leptotrichia and Veillonella were correlated with 6'-sialyl-lactose, and Porphyromonas was correlated with lacto-N-hexaose.
32526930	7	56	theme	-secretor	886:894	arg1	type					905:908	the Le(a-b-)-secretor (Le-Se+) type	874:908	the Le(a-b-)-secretor (Le-Se+) type (23%)	874:914	The Le (a-b+)-secretor (Le+Se+) type was the most abundant in Dubai mothers (60%), followed by the Le(a-b-)-secretor (Le-Se+) type (23%).
32526930	7	56	theme	-secretor	886:894	arg1	%					913:913	23%	911:913	23%	911:913	The Le (a-b+)-secretor (Le+Se+) type was the most abundant in Dubai mothers (60%), followed by the Le(a-b-)-secretor (Le-Se+) type (23%).
32526930	3	57	theme	healthy	455:461	arg1	Emirati					463:469	healthy Emirati	455:469	healthy Emirati	455:469	A total of 30 breast milk samples were collected from healthy Emirati and UAE-expatriates at Latifa Hospital.
32526930	7	58	theme	Dubai	841:845	arg1	mothers					847:853	Dubai mothers	841:853	Dubai mothers (60%)	841:859	The Le (a-b+)-secretor (Le+Se+) type was the most abundant in Dubai mothers (60%), followed by the Le(a-b-)-secretor (Le-Se+) type (23%).
32526930	7	58	theme	Dubai	841:845	arg1	%					858:858	60%	856:858	60%	856:858	The Le (a-b+)-secretor (Le+Se+) type was the most abundant in Dubai mothers (60%), followed by the Le(a-b-)-secretor (Le-Se+) type (23%).
32526930	1	59	theme	geographic	207:216	arg1	location					218:225	geographic location	207:225	geographic location	207:225	The composition of human breast milk is affected by several factors, including genetics, geographic location and maternal nutrition.
32526930	2	60	with	Dubai	349:353	arg1	microbiota					389:398	the milk microbiota	380:398	the milk microbiota	380:398	This study investigated the human milk oligosaccharides (HMOs) of breastfeeding mothers living in Dubai and their relations with the milk microbiota.
32526930	11	61	theme	environmental	1569:1581	arg1	factors					1583:1589	sociocultural and environmental factors	1551:1589	sociocultural and environmental factors	1551:1589	Targeted genomic analyses may help in determining whether these differences are due to genetic variations or to sociocultural and environmental factors.
32526930	10	62	from	profiles	1334:1341	arg1	Dubai					1371:1375	Dubai	1371:1375	Dubai	1371:1375	The study highlights the HMO profiles of breastfeeding mothers in Dubai and reveals few correlations with milk microbial composition.
32526930	2	63	with	relations	365:373	arg1	microbiota					389:398	the milk microbiota	380:398	the milk microbiota	380:398	This study investigated the human milk oligosaccharides (HMOs) of breastfeeding mothers living in Dubai and their relations with the milk microbiota.
32526930	7	64	dep	-secretor	792:800	arg1	Le+Se+					803:808	Le+Se+	803:808	Le+Se+	803:808	The Le (a-b+)-secretor (Le+Se+) type was the most abundant in Dubai mothers (60%), followed by the Le(a-b-)-secretor (Le-Se+) type (23%).
32526930	1	65	theme	human	137:141	arg1	milk					150:153	human breast milk	137:153	human breast milk	137:153	The composition of human breast milk is affected by several factors, including genetics, geographic location and maternal nutrition.
32526930	0	66	from	Dubai	111:115	arg1	Microbiota					72:81	the Milk Microbiota	63:81	the Milk Microbiota of Breastfeeding Mothers in Dubai	63:115	Profiles of Human Milk Oligosaccharides and Their Relations to the Milk Microbiota of Breastfeeding Mothers in Dubai.
31979147	6	0	theme	lipid	1079:1083	arg1	accumulation					1085:1096	lipid accumulation	1079:1096	lipid accumulation	1079:1096	High starch diet-induced dysbiosis caused increase of lipid accumulation and inflammation-related proteins' expression, thereby leading to non-alcoholic fatty liver disease.
31979147	6	1	theme	liver	1184:1188	arg1	disease					1190:1196	non-alcoholic fatty liver disease	1164:1196	non-alcoholic fatty liver disease	1164:1196	High starch diet-induced dysbiosis caused increase of lipid accumulation and inflammation-related proteins' expression, thereby leading to non-alcoholic fatty liver disease.
31979147	3	2	theme	wheat	474:478	arg1	diet					487:490	gelatinized wheat starch diet	462:490	gelatinized wheat starch diet (GWD)	462:496	However, gelatinized wheat starch diet (GWD) and NaCl-supplemented gelatinized wheat starch diet (SGW) mice did not increase body and WAT weights or dyslipidemia, and maintained consistent colon pH at ND levels.
31979147	3	2	theme	wheat	474:478	arg1	GWD					493:495	GWD	493:495	GWD	493:495	However, gelatinized wheat starch diet (GWD) and NaCl-supplemented gelatinized wheat starch diet (SGW) mice did not increase body and WAT weights or dyslipidemia, and maintained consistent colon pH at ND levels.
31979147	4	3	theme	Lactobacillus	732:744	arg1	levels					687:692	higher levels	680:692	higher levels of Desulfovibrio, Faecalibaculum, and Lactobacillus	680:744	WD mice showed higher levels of Desulfovibrio, Faecalibaculum, and Lactobacillus and lower levels of Muribaculum compared to ND mice.
31979147	4	3	theme	Lactobacillus	732:744	arg1	levels					756:761	lower levels	750:761	lower levels of Muribaculum	750:776	WD mice showed higher levels of Desulfovibrio, Faecalibaculum, and Lactobacillus and lower levels of Muribaculum compared to ND mice.
31979147	7	4	theme	gut	1296:1298	arg1	composition					1310:1320	the gut microbial composition	1292:1320	the gut microbial composition	1292:1320	However, GWD and SGW showed lower levels than that, and it might be due to the difference in the gut microbial composition compared to WD.
31979147	6	5	theme	non-alcoholic	1164:1176	arg1	disease					1190:1196	non-alcoholic fatty liver disease	1164:1196	non-alcoholic fatty liver disease	1164:1196	High starch diet-induced dysbiosis caused increase of lipid accumulation and inflammation-related proteins' expression, thereby leading to non-alcoholic fatty liver disease.
31979147	1	6	theme	gelatinized	146:156	arg1	starch					158:163	gelatinized starch	146:163	gelatinized starch	146:163	Diets high in gelatinized starch and high in gelatinized starch supplemented with salt-induced metabolic disorders and changes in gut microbiota have scarcely been studied.
31979147	4	7	theme	lower	750:754	arg1	levels					756:761	lower levels	750:761	lower levels of Muribaculum	750:776	WD mice showed higher levels of Desulfovibrio, Faecalibaculum, and Lactobacillus and lower levels of Muribaculum compared to ND mice.
31979147	6	8	theme	starch	1030:1035	arg1	dysbiosis					1050:1058	High starch diet-induced dysbiosis	1025:1058	High starch diet-induced dysbiosis	1025:1058	High starch diet-induced dysbiosis caused increase of lipid accumulation and inflammation-related proteins' expression, thereby leading to non-alcoholic fatty liver disease.
31979147	5	9	dep	such	885:888	arg1	as					890:891	as	890:891	as	890:891	However, GWD and SGW mice showed a significantly different gut microbial composition, such as a lower proportion of Lactobacillus and Desulfovibrio, and higher proportion of Faecalibaculum and Muribaculum compared to WD mice.
31979147	3	10	theme	colon	642:646	arg1	pH					648:649	consistent colon pH	631:649	consistent colon pH	631:649	However, gelatinized wheat starch diet (GWD) and NaCl-supplemented gelatinized wheat starch diet (SGW) mice did not increase body and WAT weights or dyslipidemia, and maintained consistent colon pH at ND levels.
31979147	3	11	theme	diet	487:490	arg1	mice					556:559	gelatinized wheat starch diet (GWD) and NaCl-supplemented gelatinized wheat starch diet (SGW) mice	462:559	gelatinized wheat starch diet (GWD) and NaCl-supplemented gelatinized wheat starch diet (SGW) mice	462:559	However, gelatinized wheat starch diet (GWD) and NaCl-supplemented gelatinized wheat starch diet (SGW) mice did not increase body and WAT weights or dyslipidemia, and maintained consistent colon pH at ND levels.
31979147	1	12	theme	salt-induced	183:194	arg1	disorders					206:214	salt-induced metabolic disorders	183:214	salt-induced metabolic disorders	183:214	Diets high in gelatinized starch and high in gelatinized starch supplemented with salt-induced metabolic disorders and changes in gut microbiota have scarcely been studied.
31979147	3	13	theme	gelatinized	520:530	arg1	SGW					551:553	SGW	551:553	SGW	551:553	However, gelatinized wheat starch diet (GWD) and NaCl-supplemented gelatinized wheat starch diet (SGW) mice did not increase body and WAT weights or dyslipidemia, and maintained consistent colon pH at ND levels.
31979147	3	13	theme	gelatinized	520:530	arg1	diet					545:548	NaCl-supplemented gelatinized wheat starch diet	502:548	NaCl-supplemented gelatinized wheat starch diet (SGW)	502:554	However, gelatinized wheat starch diet (GWD) and NaCl-supplemented gelatinized wheat starch diet (SGW) mice did not increase body and WAT weights or dyslipidemia, and maintained consistent colon pH at ND levels.
31979147	4	14	theme	ND	790:791	arg1	mice					793:796	ND mice	790:796	ND mice	790:796	WD mice showed higher levels of Desulfovibrio, Faecalibaculum, and Lactobacillus and lower levels of Muribaculum compared to ND mice.
31979147	5	15	theme	Lactobacillus	915:927	arg1	proportion					901:910	a lower proportion	893:910	a lower proportion of Lactobacillus and Desulfovibrio	893:945	However, GWD and SGW mice showed a significantly different gut microbial composition, such as a lower proportion of Lactobacillus and Desulfovibrio, and higher proportion of Faecalibaculum and Muribaculum compared to WD mice.
31979147	1	16	from	changes	220:226	arg1	microbiota					235:244	gut microbiota	231:244	gut microbiota	231:244	Diets high in gelatinized starch and high in gelatinized starch supplemented with salt-induced metabolic disorders and changes in gut microbiota have scarcely been studied.
31979147	5	17	theme	Desulfovibrio	933:945	arg1	proportion					901:910	a lower proportion	893:910	a lower proportion of Lactobacillus and Desulfovibrio	893:945	However, GWD and SGW mice showed a significantly different gut microbial composition, such as a lower proportion of Lactobacillus and Desulfovibrio, and higher proportion of Faecalibaculum and Muribaculum compared to WD mice.
31979147	1	18	from	starch	158:163	arg1	high					138:141	high	138:141	high	138:141	Diets high in gelatinized starch and high in gelatinized starch supplemented with salt-induced metabolic disorders and changes in gut microbiota have scarcely been studied.
31979147	1	19	theme	high	107:110	arg1	Diets					101:105	Diets	101:105	Diets high in gelatinized starch and high in gelatinized starch supplemented with salt-induced metabolic disorders and changes in gut microbiota	101:244	Diets high in gelatinized starch and high in gelatinized starch supplemented with salt-induced metabolic disorders and changes in gut microbiota have scarcely been studied.
31979147	0	20	theme	Salt	49:52	arg1	Diet					54:57	High Salt Diet	44:57	High Salt Diet	44:57	The Effects of Gelatinized Wheat Starch and High Salt Diet on Gut Microbiota and Metabolic Disorder.
31979147	3	21	theme	starch	538:543	arg1	SGW					551:553	SGW	551:553	SGW	551:553	However, gelatinized wheat starch diet (GWD) and NaCl-supplemented gelatinized wheat starch diet (SGW) mice did not increase body and WAT weights or dyslipidemia, and maintained consistent colon pH at ND levels.
31979147	3	21	theme	starch	538:543	arg1	diet					545:548	NaCl-supplemented gelatinized wheat starch diet	502:548	NaCl-supplemented gelatinized wheat starch diet (SGW)	502:554	However, gelatinized wheat starch diet (GWD) and NaCl-supplemented gelatinized wheat starch diet (SGW) mice did not increase body and WAT weights or dyslipidemia, and maintained consistent colon pH at ND levels.
31979147	1	22	theme	high	138:141	arg1	Diets					101:105	Diets	101:105	Diets high in gelatinized starch and high in gelatinized starch supplemented with salt-induced metabolic disorders and changes in gut microbiota	101:244	Diets high in gelatinized starch and high in gelatinized starch supplemented with salt-induced metabolic disorders and changes in gut microbiota have scarcely been studied.
31979147	5	23	theme	Faecalibaculum	973:986	arg1	proportion					959:968	higher proportion	952:968	higher proportion of Faecalibaculum and Muribaculum	952:1002	However, GWD and SGW mice showed a significantly different gut microbial composition, such as a lower proportion of Lactobacillus and Desulfovibrio, and higher proportion of Faecalibaculum and Muribaculum compared to WD mice.
31979147	5	24	theme	different	848:856	arg1	composition					872:882	a significantly different gut microbial composition	832:882	a significantly different gut microbial composition	832:882	However, GWD and SGW mice showed a significantly different gut microbial composition, such as a lower proportion of Lactobacillus and Desulfovibrio, and higher proportion of Faecalibaculum and Muribaculum compared to WD mice.
31979147	8	25	theme	high	1391:1394	arg1	diets					1354:1358	diets	1354:1358	diets high in gelatinized starch and high in gelatinized starch supplemented with salt	1354:1439	Taken together, diets high in gelatinized starch and high in gelatinized starch supplemented with salt induced mild metabolic disorders compared to native starch.
31979147	0	26	theme	Metabolic	81:89	arg1	Disorder					91:98	Metabolic Disorder	81:98	Metabolic Disorder	81:98	The Effects of Gelatinized Wheat Starch and High Salt Diet on Gut Microbiota and Metabolic Disorder.
31979147	5	27	theme	Muribaculum	992:1002	arg1	proportion					959:968	higher proportion	952:968	higher proportion of Faecalibaculum and Muribaculum	952:1002	However, GWD and SGW mice showed a significantly different gut microbial composition, such as a lower proportion of Lactobacillus and Desulfovibrio, and higher proportion of Faecalibaculum and Muribaculum compared to WD mice.
31979147	0	28	theme	Starch	33:38	arg1	Effects					4:10	The Effects	0:10	The Effects of Gelatinized Wheat Starch and High Salt Diet on Gut Microbiota and Metabolic Disorder	0:98	The Effects of Gelatinized Wheat Starch and High Salt Diet on Gut Microbiota and Metabolic Disorder.
31979147	1	29	from	high	107:110	arg1	starch					127:132	gelatinized starch	115:132	gelatinized starch	115:132	Diets high in gelatinized starch and high in gelatinized starch supplemented with salt-induced metabolic disorders and changes in gut microbiota have scarcely been studied.
31979147	5	30	theme	microbial	862:870	arg1	composition					872:882	a significantly different gut microbial composition	832:882	a significantly different gut microbial composition	832:882	However, GWD and SGW mice showed a significantly different gut microbial composition, such as a lower proportion of Lactobacillus and Desulfovibrio, and higher proportion of Faecalibaculum and Muribaculum compared to WD mice.
31979147	8	31	theme	gelatinized	1399:1409	arg1	starch					1411:1416	gelatinized starch	1399:1416	gelatinized starch	1399:1416	Taken together, diets high in gelatinized starch and high in gelatinized starch supplemented with salt induced mild metabolic disorders compared to native starch.
31979147	2	32	theme	body	352:355	arg1	weight					357:362	significantly higher body weight	331:362	significantly higher body weight	331:362	In this study, mice on wheat starch diets (WD) exhibited significantly higher body weight, white adipose tissue (WAT), and gut permeability compared to those on normal diet (ND).
31979147	8	33	from	starch	1380:1385	arg1	high					1360:1363	high	1360:1363	high	1360:1363	Taken together, diets high in gelatinized starch and high in gelatinized starch supplemented with salt induced mild metabolic disorders compared to native starch.
31979147	7	34	theme	lower	1227:1231	arg1	levels					1233:1238	lower levels	1227:1238	lower levels than that	1227:1248	However, GWD and SGW showed lower levels than that, and it might be due to the difference in the gut microbial composition compared to WD.
31979147	2	35	theme	adipose	371:377	arg1	tissue					379:384	white adipose tissue	365:384	white adipose tissue (WAT)	365:390	In this study, mice on wheat starch diets (WD) exhibited significantly higher body weight, white adipose tissue (WAT), and gut permeability compared to those on normal diet (ND).
31979147	0	36	theme	Wheat	27:31	arg1	Starch					33:38	Gelatinized Wheat Starch	15:38	Gelatinized Wheat Starch	15:38	The Effects of Gelatinized Wheat Starch and High Salt Diet on Gut Microbiota and Metabolic Disorder.
31979147	5	37	theme	lower	895:899	arg1	proportion					901:910	a lower proportion	893:910	a lower proportion of Lactobacillus and Desulfovibrio	893:945	However, GWD and SGW mice showed a significantly different gut microbial composition, such as a lower proportion of Lactobacillus and Desulfovibrio, and higher proportion of Faecalibaculum and Muribaculum compared to WD mice.
31979147	8	38	theme	mild	1449:1452	arg1	disorders					1464:1472	mild metabolic disorders	1449:1472	mild metabolic disorders	1449:1472	Taken together, diets high in gelatinized starch and high in gelatinized starch supplemented with salt induced mild metabolic disorders compared to native starch.
31979147	8	39	from	high	1391:1394	arg1	starch					1411:1416	gelatinized starch	1399:1416	gelatinized starch	1399:1416	Taken together, diets high in gelatinized starch and high in gelatinized starch supplemented with salt induced mild metabolic disorders compared to native starch.
31979147	4	40	theme	higher	680:685	arg1	levels					687:692	higher levels	680:692	higher levels of Desulfovibrio, Faecalibaculum, and Lactobacillus	680:744	WD mice showed higher levels of Desulfovibrio, Faecalibaculum, and Lactobacillus and lower levels of Muribaculum compared to ND mice.
31979147	2	41	theme	starch	303:308	arg1	diets					310:314	wheat starch diets	297:314	wheat starch diets (WD)	297:319	In this study, mice on wheat starch diets (WD) exhibited significantly higher body weight, white adipose tissue (WAT), and gut permeability compared to those on normal diet (ND).
31979147	2	41	theme	starch	303:308	arg1	WD					317:318	WD	317:318	WD	317:318	In this study, mice on wheat starch diets (WD) exhibited significantly higher body weight, white adipose tissue (WAT), and gut permeability compared to those on normal diet (ND).
31979147	3	42	theme	gelatinized	462:472	arg1	diet					487:490	gelatinized wheat starch diet	462:490	gelatinized wheat starch diet (GWD)	462:496	However, gelatinized wheat starch diet (GWD) and NaCl-supplemented gelatinized wheat starch diet (SGW) mice did not increase body and WAT weights or dyslipidemia, and maintained consistent colon pH at ND levels.
31979147	3	42	theme	gelatinized	462:472	arg1	GWD					493:495	GWD	493:495	GWD	493:495	However, gelatinized wheat starch diet (GWD) and NaCl-supplemented gelatinized wheat starch diet (SGW) mice did not increase body and WAT weights or dyslipidemia, and maintained consistent colon pH at ND levels.
31979147	4	43	theme	Muribaculum	766:776	arg1	levels					687:692	higher levels	680:692	higher levels of Desulfovibrio, Faecalibaculum, and Lactobacillus	680:744	WD mice showed higher levels of Desulfovibrio, Faecalibaculum, and Lactobacillus and lower levels of Muribaculum compared to ND mice.
31979147	4	43	theme	Muribaculum	766:776	arg1	levels					756:761	lower levels	750:761	lower levels of Muribaculum	750:776	WD mice showed higher levels of Desulfovibrio, Faecalibaculum, and Lactobacillus and lower levels of Muribaculum compared to ND mice.
31979147	3	44	theme	consistent	631:640	arg1	pH					648:649	consistent colon pH	631:649	consistent colon pH	631:649	However, gelatinized wheat starch diet (GWD) and NaCl-supplemented gelatinized wheat starch diet (SGW) mice did not increase body and WAT weights or dyslipidemia, and maintained consistent colon pH at ND levels.
31979147	6	45	theme	fatty	1178:1182	arg1	disease					1190:1196	non-alcoholic fatty liver disease	1164:1196	non-alcoholic fatty liver disease	1164:1196	High starch diet-induced dysbiosis caused increase of lipid accumulation and inflammation-related proteins' expression, thereby leading to non-alcoholic fatty liver disease.
31979147	7	46	theme	microbial	1300:1308	arg1	composition					1310:1320	the gut microbial composition	1292:1320	the gut microbial composition	1292:1320	However, GWD and SGW showed lower levels than that, and it might be due to the difference in the gut microbial composition compared to WD.
31979147	6	47	theme	diet-induced	1037:1048	arg1	dysbiosis					1050:1058	High starch diet-induced dysbiosis	1025:1058	High starch diet-induced dysbiosis	1025:1058	High starch diet-induced dysbiosis caused increase of lipid accumulation and inflammation-related proteins' expression, thereby leading to non-alcoholic fatty liver disease.
31979147	3	48	theme	ND	654:655	arg1	levels					657:662	ND levels	654:662	ND levels	654:662	However, gelatinized wheat starch diet (GWD) and NaCl-supplemented gelatinized wheat starch diet (SGW) mice did not increase body and WAT weights or dyslipidemia, and maintained consistent colon pH at ND levels.
31979147	3	49	theme	starch	480:485	arg1	diet					487:490	gelatinized wheat starch diet	462:490	gelatinized wheat starch diet (GWD)	462:496	However, gelatinized wheat starch diet (GWD) and NaCl-supplemented gelatinized wheat starch diet (SGW) mice did not increase body and WAT weights or dyslipidemia, and maintained consistent colon pH at ND levels.
31979147	3	49	theme	starch	480:485	arg1	GWD					493:495	GWD	493:495	GWD	493:495	However, gelatinized wheat starch diet (GWD) and NaCl-supplemented gelatinized wheat starch diet (SGW) mice did not increase body and WAT weights or dyslipidemia, and maintained consistent colon pH at ND levels.
31979147	6	50	theme	High	1025:1028	arg1	dysbiosis					1050:1058	High starch diet-induced dysbiosis	1025:1058	High starch diet-induced dysbiosis	1025:1058	High starch diet-induced dysbiosis caused increase of lipid accumulation and inflammation-related proteins' expression, thereby leading to non-alcoholic fatty liver disease.
31979147	5	51	theme	higher	952:957	arg1	proportion					959:968	higher proportion	952:968	higher proportion of Faecalibaculum and Muribaculum	952:1002	However, GWD and SGW mice showed a significantly different gut microbial composition, such as a lower proportion of Lactobacillus and Desulfovibrio, and higher proportion of Faecalibaculum and Muribaculum compared to WD mice.
31979147	3	52	theme	NaCl-supplemented	502:518	arg1	SGW					551:553	SGW	551:553	SGW	551:553	However, gelatinized wheat starch diet (GWD) and NaCl-supplemented gelatinized wheat starch diet (SGW) mice did not increase body and WAT weights or dyslipidemia, and maintained consistent colon pH at ND levels.
31979147	3	52	theme	NaCl-supplemented	502:518	arg1	diet					545:548	NaCl-supplemented gelatinized wheat starch diet	502:548	NaCl-supplemented gelatinized wheat starch diet (SGW)	502:554	However, gelatinized wheat starch diet (GWD) and NaCl-supplemented gelatinized wheat starch diet (SGW) mice did not increase body and WAT weights or dyslipidemia, and maintained consistent colon pH at ND levels.
31979147	1	53	theme	metabolic	196:204	arg1	disorders					206:214	salt-induced metabolic disorders	183:214	salt-induced metabolic disorders	183:214	Diets high in gelatinized starch and high in gelatinized starch supplemented with salt-induced metabolic disorders and changes in gut microbiota have scarcely been studied.
31979147	1	54	from	disorders	206:214	arg1	microbiota					235:244	gut microbiota	231:244	gut microbiota	231:244	Diets high in gelatinized starch and high in gelatinized starch supplemented with salt-induced metabolic disorders and changes in gut microbiota have scarcely been studied.
31979147	3	55	theme	diet	545:548	arg1	mice					556:559	gelatinized wheat starch diet (GWD) and NaCl-supplemented gelatinized wheat starch diet (SGW) mice	462:559	gelatinized wheat starch diet (GWD) and NaCl-supplemented gelatinized wheat starch diet (SGW) mice	462:559	However, gelatinized wheat starch diet (GWD) and NaCl-supplemented gelatinized wheat starch diet (SGW) mice did not increase body and WAT weights or dyslipidemia, and maintained consistent colon pH at ND levels.
31979147	5	56	theme	GWD	808:810	arg1	mice					820:823	GWD and SGW mice	808:823	GWD and SGW mice	808:823	However, GWD and SGW mice showed a significantly different gut microbial composition, such as a lower proportion of Lactobacillus and Desulfovibrio, and higher proportion of Faecalibaculum and Muribaculum compared to WD mice.
31979147	7	57	from	difference	1278:1287	arg1	composition					1310:1320	the gut microbial composition	1292:1320	the gut microbial composition	1292:1320	However, GWD and SGW showed lower levels than that, and it might be due to the difference in the gut microbial composition compared to WD.
31979147	8	58	theme	high	1360:1363	arg1	diets					1354:1358	diets	1354:1358	diets high in gelatinized starch and high in gelatinized starch supplemented with salt	1354:1439	Taken together, diets high in gelatinized starch and high in gelatinized starch supplemented with salt induced mild metabolic disorders compared to native starch.
31979147	6	59	theme	inflammation-related	1102:1121	arg1	proteins					1123:1130	inflammation-related proteins	1102:1130	inflammation-related proteins' expression	1102:1142	High starch diet-induced dysbiosis caused increase of lipid accumulation and inflammation-related proteins' expression, thereby leading to non-alcoholic fatty liver disease.
31979147	0	60	theme	Diet	54:57	arg1	Effects					4:10	The Effects	0:10	The Effects of Gelatinized Wheat Starch and High Salt Diet on Gut Microbiota and Metabolic Disorder	0:98	The Effects of Gelatinized Wheat Starch and High Salt Diet on Gut Microbiota and Metabolic Disorder.
31979147	3	61	theme	wheat	532:536	arg1	SGW					551:553	SGW	551:553	SGW	551:553	However, gelatinized wheat starch diet (GWD) and NaCl-supplemented gelatinized wheat starch diet (SGW) mice did not increase body and WAT weights or dyslipidemia, and maintained consistent colon pH at ND levels.
31979147	3	61	theme	wheat	532:536	arg1	diet					545:548	NaCl-supplemented gelatinized wheat starch diet	502:548	NaCl-supplemented gelatinized wheat starch diet (SGW)	502:554	However, gelatinized wheat starch diet (GWD) and NaCl-supplemented gelatinized wheat starch diet (SGW) mice did not increase body and WAT weights or dyslipidemia, and maintained consistent colon pH at ND levels.
31979147	1	62	theme	gelatinized	115:125	arg1	starch					127:132	gelatinized starch	115:132	gelatinized starch	115:132	Diets high in gelatinized starch and high in gelatinized starch supplemented with salt-induced metabolic disorders and changes in gut microbiota have scarcely been studied.
31979147	2	63	theme	normal	435:440	arg1	ND					448:449	ND	448:449	ND	448:449	In this study, mice on wheat starch diets (WD) exhibited significantly higher body weight, white adipose tissue (WAT), and gut permeability compared to those on normal diet (ND).
31979147	2	63	theme	normal	435:440	arg1	diet					442:445	normal diet	435:445	normal diet (ND)	435:450	In this study, mice on wheat starch diets (WD) exhibited significantly higher body weight, white adipose tissue (WAT), and gut permeability compared to those on normal diet (ND).
31979147	8	64	theme	gelatinized	1368:1378	arg1	starch					1380:1385	gelatinized starch	1368:1385	gelatinized starch	1368:1385	Taken together, diets high in gelatinized starch and high in gelatinized starch supplemented with salt induced mild metabolic disorders compared to native starch.
31979147	6	65	theme	accumulation	1085:1096	arg1	increase					1067:1074	increase	1067:1074	increase of lipid accumulation	1067:1096	High starch diet-induced dysbiosis caused increase of lipid accumulation and inflammation-related proteins' expression, thereby leading to non-alcoholic fatty liver disease.
31979147	6	65	theme	accumulation	1085:1096	arg1	expression					1133:1142	inflammation-related proteins' expression	1102:1142	inflammation-related proteins' expression	1102:1142	High starch diet-induced dysbiosis caused increase of lipid accumulation and inflammation-related proteins' expression, thereby leading to non-alcoholic fatty liver disease.
31979147	0	66	theme	Gut	62:64	arg1	Microbiota					66:75	Gut Microbiota	62:75	Gut Microbiota	62:75	The Effects of Gelatinized Wheat Starch and High Salt Diet on Gut Microbiota and Metabolic Disorder.
31979147	5	67	theme	WD	1016:1017	arg1	mice					1019:1022	WD mice	1016:1022	WD mice	1016:1022	However, GWD and SGW mice showed a significantly different gut microbial composition, such as a lower proportion of Lactobacillus and Desulfovibrio, and higher proportion of Faecalibaculum and Muribaculum compared to WD mice.
31979147	0	68	from	Effects	4:10	arg1	Microbiota					66:75	Gut Microbiota	62:75	Gut Microbiota	62:75	The Effects of Gelatinized Wheat Starch and High Salt Diet on Gut Microbiota and Metabolic Disorder.
31979147	0	68	from	Effects	4:10	arg1	Disorder					91:98	Metabolic Disorder	81:98	Metabolic Disorder	81:98	The Effects of Gelatinized Wheat Starch and High Salt Diet on Gut Microbiota and Metabolic Disorder.
31979147	8	69	from	high	1360:1363	arg1	starch					1380:1385	gelatinized starch	1368:1385	gelatinized starch	1368:1385	Taken together, diets high in gelatinized starch and high in gelatinized starch supplemented with salt induced mild metabolic disorders compared to native starch.
31979147	1	70	from	high	138:141	arg1	starch					158:163	gelatinized starch	146:163	gelatinized starch	146:163	Diets high in gelatinized starch and high in gelatinized starch supplemented with salt-induced metabolic disorders and changes in gut microbiota have scarcely been studied.
31979147	2	71	from	mice	289:292	arg1	diets					310:314	wheat starch diets	297:314	wheat starch diets (WD)	297:319	In this study, mice on wheat starch diets (WD) exhibited significantly higher body weight, white adipose tissue (WAT), and gut permeability compared to those on normal diet (ND).
31979147	2	71	from	mice	289:292	arg1	WD					317:318	WD	317:318	WD	317:318	In this study, mice on wheat starch diets (WD) exhibited significantly higher body weight, white adipose tissue (WAT), and gut permeability compared to those on normal diet (ND).
31979147	1	72	from	starch	127:132	arg1	high					107:110	high	107:110	high	107:110	Diets high in gelatinized starch and high in gelatinized starch supplemented with salt-induced metabolic disorders and changes in gut microbiota have scarcely been studied.
31979147	5	73	theme	gut	858:860	arg1	composition					872:882	a significantly different gut microbial composition	832:882	a significantly different gut microbial composition	832:882	However, GWD and SGW mice showed a significantly different gut microbial composition, such as a lower proportion of Lactobacillus and Desulfovibrio, and higher proportion of Faecalibaculum and Muribaculum compared to WD mice.
31979147	4	74	theme	WD	665:666	arg1	mice					668:671	WD mice	665:671	WD mice	665:671	WD mice showed higher levels of Desulfovibrio, Faecalibaculum, and Lactobacillus and lower levels of Muribaculum compared to ND mice.
31979147	5	75	theme	SGW	816:818	arg1	mice					820:823	GWD and SGW mice	808:823	GWD and SGW mice	808:823	However, GWD and SGW mice showed a significantly different gut microbial composition, such as a lower proportion of Lactobacillus and Desulfovibrio, and higher proportion of Faecalibaculum and Muribaculum compared to WD mice.
31979147	2	76	theme	higher	345:350	arg1	weight					357:362	significantly higher body weight	331:362	significantly higher body weight	331:362	In this study, mice on wheat starch diets (WD) exhibited significantly higher body weight, white adipose tissue (WAT), and gut permeability compared to those on normal diet (ND).
31979147	0	77	theme	Gelatinized	15:25	arg1	Starch					33:38	Gelatinized Wheat Starch	15:38	Gelatinized Wheat Starch	15:38	The Effects of Gelatinized Wheat Starch and High Salt Diet on Gut Microbiota and Metabolic Disorder.
31979147	8	78	from	starch	1411:1416	arg1	high					1391:1394	high	1391:1394	high	1391:1394	Taken together, diets high in gelatinized starch and high in gelatinized starch supplemented with salt induced mild metabolic disorders compared to native starch.
31979147	8	79	theme	metabolic	1454:1462	arg1	disorders					1464:1472	mild metabolic disorders	1449:1472	mild metabolic disorders	1449:1472	Taken together, diets high in gelatinized starch and high in gelatinized starch supplemented with salt induced mild metabolic disorders compared to native starch.
31979147	4	80	theme	Desulfovibrio	697:709	arg1	levels					687:692	higher levels	680:692	higher levels of Desulfovibrio, Faecalibaculum, and Lactobacillus	680:744	WD mice showed higher levels of Desulfovibrio, Faecalibaculum, and Lactobacillus and lower levels of Muribaculum compared to ND mice.
31979147	4	80	theme	Desulfovibrio	697:709	arg1	levels					756:761	lower levels	750:761	lower levels of Muribaculum	750:776	WD mice showed higher levels of Desulfovibrio, Faecalibaculum, and Lactobacillus and lower levels of Muribaculum compared to ND mice.
31979147	2	81	theme	white	365:369	arg1	tissue					379:384	white adipose tissue	365:384	white adipose tissue (WAT)	365:390	In this study, mice on wheat starch diets (WD) exhibited significantly higher body weight, white adipose tissue (WAT), and gut permeability compared to those on normal diet (ND).
31979147	0	82	theme	High	44:47	arg1	Diet					54:57	High Salt Diet	44:57	High Salt Diet	44:57	The Effects of Gelatinized Wheat Starch and High Salt Diet on Gut Microbiota and Metabolic Disorder.
31979147	2	83	theme	gut	397:399	arg1	permeability					401:412	gut permeability	397:412	gut permeability	397:412	In this study, mice on wheat starch diets (WD) exhibited significantly higher body weight, white adipose tissue (WAT), and gut permeability compared to those on normal diet (ND).
31979147	4	84	theme	Faecalibaculum	712:725	arg1	levels					687:692	higher levels	680:692	higher levels of Desulfovibrio, Faecalibaculum, and Lactobacillus	680:744	WD mice showed higher levels of Desulfovibrio, Faecalibaculum, and Lactobacillus and lower levels of Muribaculum compared to ND mice.
31979147	4	84	theme	Faecalibaculum	712:725	arg1	levels					756:761	lower levels	750:761	lower levels of Muribaculum	750:776	WD mice showed higher levels of Desulfovibrio, Faecalibaculum, and Lactobacillus and lower levels of Muribaculum compared to ND mice.
31979147	8	85	theme	native	1486:1491	arg1	starch					1493:1498	native starch	1486:1498	native starch	1486:1498	Taken together, diets high in gelatinized starch and high in gelatinized starch supplemented with salt induced mild metabolic disorders compared to native starch.
31979147	2	86	theme	wheat	297:301	arg1	diets					310:314	wheat starch diets	297:314	wheat starch diets (WD)	297:319	In this study, mice on wheat starch diets (WD) exhibited significantly higher body weight, white adipose tissue (WAT), and gut permeability compared to those on normal diet (ND).
31979147	2	86	theme	wheat	297:301	arg1	WD					317:318	WD	317:318	WD	317:318	In this study, mice on wheat starch diets (WD) exhibited significantly higher body weight, white adipose tissue (WAT), and gut permeability compared to those on normal diet (ND).
31979147	1	87	theme	gut	231:233	arg1	microbiota					235:244	gut microbiota	231:244	gut microbiota	231:244	Diets high in gelatinized starch and high in gelatinized starch supplemented with salt-induced metabolic disorders and changes in gut microbiota have scarcely been studied.
34737131	11	0	from	feed	2167:2170	arg1	supplementation					2119:2133	supplementation	2119:2133	supplementation of 5-15 g kg-1 trehalose in the feed	2119:2170	In addition, supplementation of 5-15 g kg-1 trehalose in the feed could improve the survival rate of red claw crayfish under desiccation stress.
34737131	10	1	theme	GPx	1914:1916	arg1	levels					1904:1909	the expression levels	1889:1909	the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA	1889:2103	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	6	2	dep	Diet	1167:1170	arg1	groups					1194:1199	groups	1194:1199	groups	1194:1199	The activities of superoxide dismutase (SOD) and alkaline phosphatase (AKP) in hepatopancreas and hemolymph of crayfish for Diet 4, Diet 5, and Diet 6 groups were significantly increased while malondialdehyde (MDA) content was significantly reduced when compared with the control.
34737131	4	3	theme	crayfish	797:804	arg1	SGR					789:791	SGR	789:791	SGR	789:791	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	4	3	theme	crayfish	797:804	arg1	rate					783:786	specific growth rate	767:786	specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups	767:840	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	4	3	theme	crayfish	797:804	arg1	WGR					758:760	WGR	758:760	WGR	758:760	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	4	3	theme	crayfish	797:804	arg1	rate					752:755	the weight gain rate	736:755	the weight gain rate (WGR)	736:761	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	11	4	theme	claw	2211:2214	arg1	crayfish					2216:2223	red claw crayfish	2207:2223	red claw crayfish	2207:2223	In addition, supplementation of 5-15 g kg-1 trehalose in the feed could improve the survival rate of red claw crayfish under desiccation stress.
34737131	0	5	theme	Cherax	156:161	arg1	quadricarinatus					163:177	juvenile red claw crayfish (Cherax quadricarinatus)	128:178	juvenile red claw crayfish (Cherax quadricarinatus)	128:178	Effects of dietary trehalose on growth, trehalose content, non-specific immunity, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	12	6	theme	5-15 g kg-1	2299:2309	arg1	trehalose					2311:2319	5-15 g kg-1 trehalose	2299:2319	5-15 g kg-1 trehalose in feed	2299:2327	These results suggested that supplementation of 5-15 g kg-1 trehalose in feed could significantly improve the growth performance, muscle protein, non-specific immunity and desiccation resistance of juvenile red claw crayfish.
34737131	1	7	theme	dietary	236:242	arg1	trehalose					244:252	dietary trehalose	236:252	dietary trehalose	236:252	This study was performed to investigate the effects of dietary trehalose on growth, muscle composition, non-specific immune responses, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	11	8	from	supplementation	2119:2133	arg1	feed					2167:2170	the feed	2163:2170	the feed	2163:2170	In addition, supplementation of 5-15 g kg-1 trehalose in the feed could improve the survival rate of red claw crayfish under desiccation stress.
34737131	10	9	theme	mRNA	2100:2103	arg1	levels					1904:1909	the expression levels	1889:1909	the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA	1889:2103	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	9	10	theme	trehalose	1772:1780	arg1	levels					1782:1787	dietary trehalose levels	1764:1787	dietary trehalose levels	1764:1787	The hepatopancreas and intestine trehalose contents of crayfish showed an upward trend with the increase of dietary trehalose levels.
34737131	10	11	theme	C-type	1919:1924	arg1	C-LZM					1936:1940	C-LZM	1936:1940	C-LZM	1936:1940	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	10	11	theme	C-type	1919:1924	arg1	lysozyme					1926:1933	C-type lysozyme	1919:1933	C-type lysozyme (C-LZM)	1919:1941	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	0	12	theme	red	137:139	arg1	quadricarinatus					163:177	juvenile red claw crayfish (Cherax quadricarinatus)	128:178	juvenile red claw crayfish (Cherax quadricarinatus)	128:178	Effects of dietary trehalose on growth, trehalose content, non-specific immunity, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	10	13	theme	Tret1-4	2091:2097	arg1	mRNA					2100:2103	facilitated trehalose transporter isoform X4 (Tret1-4) mRNA	2045:2103	facilitated trehalose transporter isoform X4 (Tret1-4) mRNA	2045:2103	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	2	14	theme	feeding	512:518	arg1	experiment					520:529	a feeding experiment	510:529	a feeding experiment	510:529	A total of 540 (body weight of 0.41 ± 0.05) crayfish were randomly divided into six groups for a feeding experiment.
34737131	0	15	theme	desiccation	102:112	arg1	resistance					114:123	desiccation resistance	102:123	desiccation resistance	102:123	Effects of dietary trehalose on growth, trehalose content, non-specific immunity, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	1	16	theme	red	371:373	arg1	quadricarinatus					397:411	juvenile red claw crayfish (Cherax quadricarinatus)	362:412	juvenile red claw crayfish (Cherax quadricarinatus)	362:412	This study was performed to investigate the effects of dietary trehalose on growth, muscle composition, non-specific immune responses, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	11	17	theme	trehalose	2150:2158	arg1	supplementation					2119:2133	supplementation	2119:2133	supplementation of 5-15 g kg-1 trehalose in the feed	2119:2170	In addition, supplementation of 5-15 g kg-1 trehalose in the feed could improve the survival rate of red claw crayfish under desiccation stress.
34737131	10	18	from	supplementation	1823:1837	arg1	feed					1871:1874	the feed	1867:1874	the feed	1867:1874	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	5	19	theme	crude	919:923	arg1	contents					933:940	Muscle crude protein contents	912:940	Muscle crude protein contents of crayfish fed Diet 4, Diet 5 and Diet 6	912:982	Muscle crude protein contents of crayfish fed Diet 4, Diet 5 and Diet 6 were significantly higher than those of the control group.
34737131	1	20	theme	crayfish	380:387	arg1	quadricarinatus					397:411	juvenile red claw crayfish (Cherax quadricarinatus)	362:412	juvenile red claw crayfish (Cherax quadricarinatus)	362:412	This study was performed to investigate the effects of dietary trehalose on growth, muscle composition, non-specific immune responses, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	4	21	from	Diet	828:831	arg1	SGR					789:791	SGR	789:791	SGR	789:791	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	4	21	from	Diet	828:831	arg1	rate					783:786	specific growth rate	767:786	specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups	767:840	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	4	21	from	Diet	828:831	arg1	WGR					758:760	WGR	758:760	WGR	758:760	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	4	21	from	Diet	828:831	arg1	rate					752:755	the weight gain rate	736:755	the weight gain rate (WGR)	736:761	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	1	22	from	effects	225:231	arg1	expression					321:330	gene expression	316:330	gene expression	316:330	This study was performed to investigate the effects of dietary trehalose on growth, muscle composition, non-specific immune responses, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	1	22	from	effects	225:231	arg1	resistance					348:357	desiccation resistance	336:357	desiccation resistance	336:357	This study was performed to investigate the effects of dietary trehalose on growth, muscle composition, non-specific immune responses, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	1	22	from	effects	225:231	arg1	growth					257:262	growth	257:262	growth	257:262	This study was performed to investigate the effects of dietary trehalose on growth, muscle composition, non-specific immune responses, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	1	22	from	effects	225:231	arg1	composition					272:282	muscle composition	265:282	muscle composition	265:282	This study was performed to investigate the effects of dietary trehalose on growth, muscle composition, non-specific immune responses, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	1	22	from	effects	225:231	arg1	responses					305:313	non-specific immune responses	285:313	non-specific immune responses	285:313	This study was performed to investigate the effects of dietary trehalose on growth, muscle composition, non-specific immune responses, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	1	23	theme	Cherax	390:395	arg1	quadricarinatus					397:411	juvenile red claw crayfish (Cherax quadricarinatus)	362:412	juvenile red claw crayfish (Cherax quadricarinatus)	362:412	This study was performed to investigate the effects of dietary trehalose on growth, muscle composition, non-specific immune responses, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	3	24	theme	trehalose	547:555	arg1	levels					557:562	trehalose levels	547:562	trehalose levels	547:562	Six diets with trehalose levels at 0 (Diet 1), 1 (Diet 2), 2 (Diet 3), 5 (Diet 4), 10 (Diet 5) and 15 (Diet 6) g kg-1 were prepared to feed juvenile red claw crayfish for 8 weeks.
34737131	12	25	theme	trehalose	2311:2319	arg1	supplementation					2280:2294	supplementation	2280:2294	supplementation of 5-15 g kg-1 trehalose in feed	2280:2327	These results suggested that supplementation of 5-15 g kg-1 trehalose in feed could significantly improve the growth performance, muscle protein, non-specific immunity and desiccation resistance of juvenile red claw crayfish.
34737131	8	26	theme	acid	1561:1564	arg1	ACP					1579:1581	ACP	1579:1581	ACP	1579:1581	However, acid phosphatase (ACP) activity was not significantly different among all experimental groups.
34737131	8	26	theme	acid	1561:1564	arg1	phosphatase					1566:1576	acid phosphatase	1561:1576	acid phosphatase (ACP) activity	1561:1591	However, acid phosphatase (ACP) activity was not significantly different among all experimental groups.
34737131	7	27	theme	total	1328:1332	arg1	capacity					1346:1353	The total antioxidant capacity	1324:1353	The total antioxidant capacity (T-AOC)	1324:1361	The total antioxidant capacity (T-AOC), catalase (CAT) and glutathione peroxidase (GPx) activities in the hepatopancreas and hemolymph of crayfish fed Diet 5 and Diet 6 were significantly higher than those in the control group.
34737131	7	27	theme	total	1328:1332	arg1	higher					1512:1517	higher	1512:1517	higher	1512:1517	The total antioxidant capacity (T-AOC), catalase (CAT) and glutathione peroxidase (GPx) activities in the hepatopancreas and hemolymph of crayfish fed Diet 5 and Diet 6 were significantly higher than those in the control group.
34737131	7	27	theme	total	1328:1332	arg1	T-AOC					1356:1360	T-AOC	1356:1360	T-AOC	1356:1360	The total antioxidant capacity (T-AOC), catalase (CAT) and glutathione peroxidase (GPx) activities in the hepatopancreas and hemolymph of crayfish fed Diet 5 and Diet 6 were significantly higher than those in the control group.
34737131	10	28	theme	transporter	2000:2010	arg1	Tret1-2					2032:2038	Tret1-2	2032:2038	Tret1-2	2032:2038	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	10	28	theme	transporter	2000:2010	arg1	X2					2028:2029	facilitated trehalose transporter homolog isoform X2	1978:2029	facilitated trehalose transporter homolog isoform X2 (Tret1-2)	1978:2039	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	5	29	theme	protein	925:931	arg1	contents					933:940	Muscle crude protein contents	912:940	Muscle crude protein contents of crayfish fed Diet 4, Diet 5 and Diet 6	912:982	Muscle crude protein contents of crayfish fed Diet 4, Diet 5 and Diet 6 were significantly higher than those of the control group.
34737131	8	30	theme	experimental	1635:1646	arg1	groups					1648:1653	all experimental groups	1631:1653	all experimental groups	1631:1653	However, acid phosphatase (ACP) activity was not significantly different among all experimental groups.
34737131	4	31	from	Diet	809:812	arg1	SGR					789:791	SGR	789:791	SGR	789:791	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	4	31	from	Diet	809:812	arg1	rate					783:786	specific growth rate	767:786	specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups	767:840	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	4	31	from	Diet	809:812	arg1	WGR					758:760	WGR	758:760	WGR	758:760	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	4	31	from	Diet	809:812	arg1	rate					752:755	the weight gain rate	736:755	the weight gain rate (WGR)	736:761	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	7	32	theme	catalase	1364:1371	arg1	activities					1412:1421	catalase (CAT) and glutathione peroxidase (GPx) activities	1364:1421	catalase (CAT) and glutathione peroxidase (GPx) activities in the hepatopancreas	1364:1443	The total antioxidant capacity (T-AOC), catalase (CAT) and glutathione peroxidase (GPx) activities in the hepatopancreas and hemolymph of crayfish fed Diet 5 and Diet 6 were significantly higher than those in the control group.
34737131	10	33	theme	facilitated	1978:1988	arg1	Tret1-2					2032:2038	Tret1-2	2032:2038	Tret1-2	2032:2038	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	10	33	theme	facilitated	1978:1988	arg1	X2					2028:2029	facilitated trehalose transporter homolog isoform X2	1978:2029	facilitated trehalose transporter homolog isoform X2 (Tret1-2)	1978:2039	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	12	34	from	feed	2324:2327	arg1	supplementation					2280:2294	supplementation	2280:2294	supplementation of 5-15 g kg-1 trehalose in feed	2280:2327	These results suggested that supplementation of 5-15 g kg-1 trehalose in feed could significantly improve the growth performance, muscle protein, non-specific immunity and desiccation resistance of juvenile red claw crayfish.
34737131	0	35	theme	crayfish	146:153	arg1	quadricarinatus					163:177	juvenile red claw crayfish (Cherax quadricarinatus)	128:178	juvenile red claw crayfish (Cherax quadricarinatus)	128:178	Effects of dietary trehalose on growth, trehalose content, non-specific immunity, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	0	36	theme	non-specific	59:70	arg1	immunity					72:79	non-specific immunity	59:79	non-specific immunity	59:79	Effects of dietary trehalose on growth, trehalose content, non-specific immunity, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	4	37	from	crayfish	797:804	arg1	Diet					809:812	Diet 4	809:814	Diet 4	809:814	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	4	37	from	crayfish	797:804	arg1	Diet					817:820	Diet 5	817:822	Diet 5	817:822	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	4	37	from	crayfish	797:804	arg1	Diet					828:831	Diet	828:831	Diet	828:831	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	12	38	theme	crayfish	2467:2474	arg1	performance					2368:2378	growth performance	2361:2378	growth performance	2361:2378	These results suggested that supplementation of 5-15 g kg-1 trehalose in feed could significantly improve the growth performance, muscle protein, non-specific immunity and desiccation resistance of juvenile red claw crayfish.
34737131	12	38	theme	crayfish	2467:2474	arg1	protein					2388:2394	muscle protein	2381:2394	muscle protein	2381:2394	These results suggested that supplementation of 5-15 g kg-1 trehalose in feed could significantly improve the growth performance, muscle protein, non-specific immunity and desiccation resistance of juvenile red claw crayfish.
34737131	12	38	theme	crayfish	2467:2474	arg1	immunity					2410:2417	non-specific immunity	2397:2417	non-specific immunity	2397:2417	These results suggested that supplementation of 5-15 g kg-1 trehalose in feed could significantly improve the growth performance, muscle protein, non-specific immunity and desiccation resistance of juvenile red claw crayfish.
34737131	12	38	theme	crayfish	2467:2474	arg1	resistance					2435:2444	desiccation resistance	2423:2444	desiccation resistance	2423:2444	These results suggested that supplementation of 5-15 g kg-1 trehalose in feed could significantly improve the growth performance, muscle protein, non-specific immunity and desiccation resistance of juvenile red claw crayfish.
34737131	0	39	from	Effects	0:6	arg1	growth					32:37	growth	32:37	growth	32:37	Effects of dietary trehalose on growth, trehalose content, non-specific immunity, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	0	39	from	Effects	0:6	arg1	content					50:56	trehalose content	40:56	trehalose content	40:56	Effects of dietary trehalose on growth, trehalose content, non-specific immunity, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	0	39	from	Effects	0:6	arg1	immunity					72:79	non-specific immunity	59:79	non-specific immunity	59:79	Effects of dietary trehalose on growth, trehalose content, non-specific immunity, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	0	39	from	Effects	0:6	arg1	expression					87:96	gene expression	82:96	gene expression	82:96	Effects of dietary trehalose on growth, trehalose content, non-specific immunity, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	0	39	from	Effects	0:6	arg1	resistance					114:123	desiccation resistance	102:123	desiccation resistance	102:123	Effects of dietary trehalose on growth, trehalose content, non-specific immunity, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	10	40	theme	trehalose	1854:1862	arg1	supplementation					1823:1837	supplementation	1823:1837	supplementation of 5-15 g kg-1 trehalose in the feed	1823:1874	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	1	41	theme	desiccation	336:346	arg1	resistance					348:357	desiccation resistance	336:357	desiccation resistance	336:357	This study was performed to investigate the effects of dietary trehalose on growth, muscle composition, non-specific immune responses, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	4	42	theme	gain	747:750	arg1	WGR					758:760	WGR	758:760	WGR	758:760	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	4	42	theme	gain	747:750	arg1	rate					752:755	the weight gain rate	736:755	the weight gain rate (WGR)	736:761	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	10	43	theme	X4	2087:2088	arg1	mRNA					2100:2103	facilitated trehalose transporter isoform X4 (Tret1-4) mRNA	2045:2103	facilitated trehalose transporter isoform X4 (Tret1-4) mRNA	2045:2103	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	7	44	theme	antioxidant	1334:1344	arg1	capacity					1346:1353	The total antioxidant capacity	1324:1353	The total antioxidant capacity (T-AOC)	1324:1361	The total antioxidant capacity (T-AOC), catalase (CAT) and glutathione peroxidase (GPx) activities in the hepatopancreas and hemolymph of crayfish fed Diet 5 and Diet 6 were significantly higher than those in the control group.
34737131	7	44	theme	antioxidant	1334:1344	arg1	higher					1512:1517	higher	1512:1517	higher	1512:1517	The total antioxidant capacity (T-AOC), catalase (CAT) and glutathione peroxidase (GPx) activities in the hepatopancreas and hemolymph of crayfish fed Diet 5 and Diet 6 were significantly higher than those in the control group.
34737131	7	44	theme	antioxidant	1334:1344	arg1	T-AOC					1356:1360	T-AOC	1356:1360	T-AOC	1356:1360	The total antioxidant capacity (T-AOC), catalase (CAT) and glutathione peroxidase (GPx) activities in the hepatopancreas and hemolymph of crayfish fed Diet 5 and Diet 6 were significantly higher than those in the control group.
34737131	7	45	from	hemolymph	1449:1457	arg1	hepatopancreas					1430:1443	the hepatopancreas	1426:1443	the hepatopancreas	1426:1443	The total antioxidant capacity (T-AOC), catalase (CAT) and glutathione peroxidase (GPx) activities in the hepatopancreas and hemolymph of crayfish fed Diet 5 and Diet 6 were significantly higher than those in the control group.
34737131	10	46	theme	facilitated	2045:2055	arg1	mRNA					2100:2103	facilitated trehalose transporter isoform X4 (Tret1-4) mRNA	2045:2103	facilitated trehalose transporter isoform X4 (Tret1-4) mRNA	2045:2103	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	12	47	theme	muscle	2381:2386	arg1	protein					2388:2394	muscle protein	2381:2394	muscle protein	2381:2394	These results suggested that supplementation of 5-15 g kg-1 trehalose in feed could significantly improve the growth performance, muscle protein, non-specific immunity and desiccation resistance of juvenile red claw crayfish.
34737131	7	48	theme	control	1537:1543	arg1	group					1545:1549	the control group	1533:1549	the control group	1533:1549	The total antioxidant capacity (T-AOC), catalase (CAT) and glutathione peroxidase (GPx) activities in the hepatopancreas and hemolymph of crayfish fed Diet 5 and Diet 6 were significantly higher than those in the control group.
34737131	12	49	theme	red	2458:2460	arg1	crayfish					2467:2474	juvenile red claw crayfish	2449:2474	juvenile red claw crayfish	2449:2474	These results suggested that supplementation of 5-15 g kg-1 trehalose in feed could significantly improve the growth performance, muscle protein, non-specific immunity and desiccation resistance of juvenile red claw crayfish.
34737131	6	50	theme	superoxide	1061:1070	arg1	dismutase					1072:1080	superoxide dismutase	1061:1080	superoxide dismutase (SOD)	1061:1086	The activities of superoxide dismutase (SOD) and alkaline phosphatase (AKP) in hepatopancreas and hemolymph of crayfish for Diet 4, Diet 5, and Diet 6 groups were significantly increased while malondialdehyde (MDA) content was significantly reduced when compared with the control.
34737131	6	50	theme	superoxide	1061:1070	arg1	SOD					1083:1085	SOD	1083:1085	SOD	1083:1085	The activities of superoxide dismutase (SOD) and alkaline phosphatase (AKP) in hepatopancreas and hemolymph of crayfish for Diet 4, Diet 5, and Diet 6 groups were significantly increased while malondialdehyde (MDA) content was significantly reduced when compared with the control.
34737131	0	51	theme	dietary	11:17	arg1	trehalose					19:27	dietary trehalose	11:27	dietary trehalose	11:27	Effects of dietary trehalose on growth, trehalose content, non-specific immunity, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	1	52	theme	muscle	265:270	arg1	composition					272:282	muscle composition	265:282	muscle composition	265:282	This study was performed to investigate the effects of dietary trehalose on growth, muscle composition, non-specific immune responses, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	4	53	theme	specific	767:774	arg1	SGR					789:791	SGR	789:791	SGR	789:791	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	4	53	theme	specific	767:774	arg1	rate					783:786	specific growth rate	767:786	specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups	767:840	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	11	54	theme	desiccation	2231:2241	arg1	stress					2243:2248	desiccation stress	2231:2248	desiccation stress	2231:2248	In addition, supplementation of 5-15 g kg-1 trehalose in the feed could improve the survival rate of red claw crayfish under desiccation stress.
34737131	9	55	theme	trehalose	1689:1697	arg1	contents					1699:1706	The hepatopancreas and intestine trehalose contents	1656:1706	The hepatopancreas and intestine trehalose contents of crayfish	1656:1718	The hepatopancreas and intestine trehalose contents of crayfish showed an upward trend with the increase of dietary trehalose levels.
34737131	6	56	theme	alkaline	1092:1099	arg1	AKP					1114:1116	AKP	1114:1116	AKP	1114:1116	The activities of superoxide dismutase (SOD) and alkaline phosphatase (AKP) in hepatopancreas and hemolymph of crayfish for Diet 4, Diet 5, and Diet 6 groups were significantly increased while malondialdehyde (MDA) content was significantly reduced when compared with the control.
34737131	6	56	theme	alkaline	1092:1099	arg1	phosphatase					1101:1111	alkaline phosphatase	1092:1111	alkaline phosphatase (AKP)	1092:1117	The activities of superoxide dismutase (SOD) and alkaline phosphatase (AKP) in hepatopancreas and hemolymph of crayfish for Diet 4, Diet 5, and Diet 6 groups were significantly increased while malondialdehyde (MDA) content was significantly reduced when compared with the control.
34737131	4	57	from	rate	783:786	arg1	Diet					809:812	Diet 4	809:814	Diet 4	809:814	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	4	57	from	rate	783:786	arg1	Diet					817:820	Diet 5	817:822	Diet 5	817:822	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	4	57	from	rate	783:786	arg1	Diet					828:831	Diet	828:831	Diet	828:831	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	12	58	theme	non-specific	2397:2408	arg1	immunity					2410:2417	non-specific immunity	2397:2417	non-specific immunity	2397:2417	These results suggested that supplementation of 5-15 g kg-1 trehalose in feed could significantly improve the growth performance, muscle protein, non-specific immunity and desiccation resistance of juvenile red claw crayfish.
34737131	11	59	from	trehalose	2150:2158	arg1	feed					2167:2170	the feed	2163:2170	the feed	2163:2170	In addition, supplementation of 5-15 g kg-1 trehalose in the feed could improve the survival rate of red claw crayfish under desiccation stress.
34737131	2	60	theme	body	431:434	arg1	weight					436:441	body weight	431:441	body weight of 0.41 ± 0.05	431:456	A total of 540 (body weight of 0.41 ± 0.05) crayfish were randomly divided into six groups for a feeding experiment.
34737131	12	61	from	trehalose	2311:2319	arg1	feed					2324:2327	feed	2324:2327	feed	2324:2327	These results suggested that supplementation of 5-15 g kg-1 trehalose in feed could significantly improve the growth performance, muscle protein, non-specific immunity and desiccation resistance of juvenile red claw crayfish.
34737131	10	62	theme	trehalose	1990:1998	arg1	Tret1-2					2032:2038	Tret1-2	2032:2038	Tret1-2	2032:2038	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	10	62	theme	trehalose	1990:1998	arg1	X2					2028:2029	facilitated trehalose transporter homolog isoform X2	1978:2029	facilitated trehalose transporter homolog isoform X2 (Tret1-2)	1978:2039	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	1	63	theme	immune	298:303	arg1	responses					305:313	non-specific immune responses	285:313	non-specific immune responses	285:313	This study was performed to investigate the effects of dietary trehalose on growth, muscle composition, non-specific immune responses, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	2	64	dep	crayfish	459:466	arg1	weight					436:441	body weight	431:441	body weight of 0.41 ± 0.05	431:456	A total of 540 (body weight of 0.41 ± 0.05) crayfish were randomly divided into six groups for a feeding experiment.
34737131	9	65	theme	upward	1730:1735	arg1	trend					1737:1741	an upward trend	1727:1741	an upward trend with the increase of dietary trehalose levels	1727:1787	The hepatopancreas and intestine trehalose contents of crayfish showed an upward trend with the increase of dietary trehalose levels.
34737131	3	66	theme	red	681:683	arg1	crayfish					690:697	juvenile red claw crayfish	672:697	juvenile red claw crayfish	672:697	Six diets with trehalose levels at 0 (Diet 1), 1 (Diet 2), 2 (Diet 3), 5 (Diet 4), 10 (Diet 5) and 15 (Diet 6) g kg-1 were prepared to feed juvenile red claw crayfish for 8 weeks.
34737131	6	67	theme	crayfish	1154:1161	arg1	hemolymph					1141:1149	hemolymph	1141:1149	hemolymph	1141:1149	The activities of superoxide dismutase (SOD) and alkaline phosphatase (AKP) in hepatopancreas and hemolymph of crayfish for Diet 4, Diet 5, and Diet 6 groups were significantly increased while malondialdehyde (MDA) content was significantly reduced when compared with the control.
34737131	6	67	theme	crayfish	1154:1161	arg1	hepatopancreas					1122:1135	hepatopancreas	1122:1135	hepatopancreas	1122:1135	The activities of superoxide dismutase (SOD) and alkaline phosphatase (AKP) in hepatopancreas and hemolymph of crayfish for Diet 4, Diet 5, and Diet 6 groups were significantly increased while malondialdehyde (MDA) content was significantly reduced when compared with the control.
34737131	3	68	dep	1	579:579	arg1	kg-1					645:648	g kg-1	643:648	g kg-1	643:648	Six diets with trehalose levels at 0 (Diet 1), 1 (Diet 2), 2 (Diet 3), 5 (Diet 4), 10 (Diet 5) and 15 (Diet 6) g kg-1 were prepared to feed juvenile red claw crayfish for 8 weeks.
34737131	10	69	theme	transporter	2067:2077	arg1	mRNA					2100:2103	facilitated trehalose transporter isoform X4 (Tret1-4) mRNA	2045:2103	facilitated trehalose transporter isoform X4 (Tret1-4) mRNA	2045:2103	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	0	70	theme	quadricarinatus	163:177	arg1	growth					32:37	growth	32:37	growth	32:37	Effects of dietary trehalose on growth, trehalose content, non-specific immunity, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	0	70	theme	quadricarinatus	163:177	arg1	content					50:56	trehalose content	40:56	trehalose content	40:56	Effects of dietary trehalose on growth, trehalose content, non-specific immunity, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	0	70	theme	quadricarinatus	163:177	arg1	immunity					72:79	non-specific immunity	59:79	non-specific immunity	59:79	Effects of dietary trehalose on growth, trehalose content, non-specific immunity, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	0	70	theme	quadricarinatus	163:177	arg1	expression					87:96	gene expression	82:96	gene expression	82:96	Effects of dietary trehalose on growth, trehalose content, non-specific immunity, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	0	70	theme	quadricarinatus	163:177	arg1	resistance					114:123	desiccation resistance	102:123	desiccation resistance	102:123	Effects of dietary trehalose on growth, trehalose content, non-specific immunity, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	5	71	theme	Muscle	912:917	arg1	contents					933:940	Muscle crude protein contents	912:940	Muscle crude protein contents of crayfish fed Diet 4, Diet 5 and Diet 6	912:982	Muscle crude protein contents of crayfish fed Diet 4, Diet 5 and Diet 6 were significantly higher than those of the control group.
34737131	9	72	with	trend	1737:1741	arg1	increase					1752:1759	the increase	1748:1759	the increase of dietary trehalose levels	1748:1787	The hepatopancreas and intestine trehalose contents of crayfish showed an upward trend with the increase of dietary trehalose levels.
34737131	11	73	theme	crayfish	2216:2223	arg1	rate					2199:2202	the survival rate	2186:2202	the survival rate of red claw crayfish	2186:2223	In addition, supplementation of 5-15 g kg-1 trehalose in the feed could improve the survival rate of red claw crayfish under desiccation stress.
34737131	10	74	theme	control	1808:1814	arg1	group					1816:1820	the control group	1804:1820	the control group	1804:1820	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	1	75	theme	trehalose	244:252	arg1	effects					225:231	the effects	221:231	the effects of dietary trehalose on growth, muscle composition, non-specific immune responses, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus)	221:412	This study was performed to investigate the effects of dietary trehalose on growth, muscle composition, non-specific immune responses, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	10	76	theme	expression	1893:1902	arg1	levels					1904:1909	the expression levels	1889:1909	the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA	1889:2103	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	11	77	theme	red	2207:2209	arg1	crayfish					2216:2223	red claw crayfish	2207:2223	red claw crayfish	2207:2223	In addition, supplementation of 5-15 g kg-1 trehalose in the feed could improve the survival rate of red claw crayfish under desiccation stress.
34737131	9	78	theme	dietary	1764:1770	arg1	levels					1782:1787	dietary trehalose levels	1764:1787	dietary trehalose levels	1764:1787	The hepatopancreas and intestine trehalose contents of crayfish showed an upward trend with the increase of dietary trehalose levels.
34737131	10	79	theme	trehalose	2057:2065	arg1	mRNA					2100:2103	facilitated trehalose transporter isoform X4 (Tret1-4) mRNA	2045:2103	facilitated trehalose transporter isoform X4 (Tret1-4) mRNA	2045:2103	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	10	80	theme	lysozyme	1926:1933	arg1	levels					1904:1909	the expression levels	1889:1909	the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA	1889:2103	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	9	81	theme	levels	1782:1787	arg1	increase					1752:1759	the increase	1748:1759	the increase of dietary trehalose levels	1748:1787	The hepatopancreas and intestine trehalose contents of crayfish showed an upward trend with the increase of dietary trehalose levels.
34737131	12	82	theme	growth	2361:2366	arg1	performance					2368:2378	growth performance	2361:2378	growth performance	2361:2378	These results suggested that supplementation of 5-15 g kg-1 trehalose in feed could significantly improve the growth performance, muscle protein, non-specific immunity and desiccation resistance of juvenile red claw crayfish.
34737131	7	83	theme	peroxidase	1395:1404	arg1	activities					1412:1421	catalase (CAT) and glutathione peroxidase (GPx) activities	1364:1421	catalase (CAT) and glutathione peroxidase (GPx) activities in the hepatopancreas	1364:1443	The total antioxidant capacity (T-AOC), catalase (CAT) and glutathione peroxidase (GPx) activities in the hepatopancreas and hemolymph of crayfish fed Diet 5 and Diet 6 were significantly higher than those in the control group.
34737131	11	84	theme	5-15 g kg-1	2138:2148	arg1	trehalose					2150:2158	5-15 g kg-1 trehalose	2138:2158	5-15 g kg-1 trehalose in the feed	2138:2170	In addition, supplementation of 5-15 g kg-1 trehalose in the feed could improve the survival rate of red claw crayfish under desiccation stress.
34737131	0	85	theme	juvenile	128:135	arg1	quadricarinatus					163:177	juvenile red claw crayfish (Cherax quadricarinatus)	128:178	juvenile red claw crayfish (Cherax quadricarinatus)	128:178	Effects of dietary trehalose on growth, trehalose content, non-specific immunity, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	1	86	theme	claw	375:378	arg1	quadricarinatus					397:411	juvenile red claw crayfish (Cherax quadricarinatus)	362:412	juvenile red claw crayfish (Cherax quadricarinatus)	362:412	This study was performed to investigate the effects of dietary trehalose on growth, muscle composition, non-specific immune responses, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	0	87	theme	claw	141:144	arg1	quadricarinatus					163:177	juvenile red claw crayfish (Cherax quadricarinatus)	128:178	juvenile red claw crayfish (Cherax quadricarinatus)	128:178	Effects of dietary trehalose on growth, trehalose content, non-specific immunity, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	10	88	theme	antilipolysacchride	1944:1962	arg1	ALF					1972:1974	ALF	1972:1974	ALF	1972:1974	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	10	88	theme	antilipolysacchride	1944:1962	arg1	factor					1964:1969	antilipolysacchride factor	1944:1969	antilipolysacchride factor (ALF)	1944:1975	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	8	89	theme	phosphatase	1566:1576	arg1	activity					1584:1591	acid phosphatase (ACP) activity	1561:1591	acid phosphatase (ACP) activity	1561:1591	However, acid phosphatase (ACP) activity was not significantly different among all experimental groups.
34737131	1	90	theme	quadricarinatus	397:411	arg1	expression					321:330	gene expression	316:330	gene expression	316:330	This study was performed to investigate the effects of dietary trehalose on growth, muscle composition, non-specific immune responses, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	1	90	theme	quadricarinatus	397:411	arg1	resistance					348:357	desiccation resistance	336:357	desiccation resistance	336:357	This study was performed to investigate the effects of dietary trehalose on growth, muscle composition, non-specific immune responses, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	1	90	theme	quadricarinatus	397:411	arg1	growth					257:262	growth	257:262	growth	257:262	This study was performed to investigate the effects of dietary trehalose on growth, muscle composition, non-specific immune responses, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	1	90	theme	quadricarinatus	397:411	arg1	composition					272:282	muscle composition	265:282	muscle composition	265:282	This study was performed to investigate the effects of dietary trehalose on growth, muscle composition, non-specific immune responses, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	1	90	theme	quadricarinatus	397:411	arg1	responses					305:313	non-specific immune responses	285:313	non-specific immune responses	285:313	This study was performed to investigate the effects of dietary trehalose on growth, muscle composition, non-specific immune responses, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	6	91	theme	malondialdehyde	1236:1250	arg1	content					1258:1264	malondialdehyde (MDA) content	1236:1264	malondialdehyde (MDA) content	1236:1264	The activities of superoxide dismutase (SOD) and alkaline phosphatase (AKP) in hepatopancreas and hemolymph of crayfish for Diet 4, Diet 5, and Diet 6 groups were significantly increased while malondialdehyde (MDA) content was significantly reduced when compared with the control.
34737131	4	92	from	Diet	817:820	arg1	SGR					789:791	SGR	789:791	SGR	789:791	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	4	92	from	Diet	817:820	arg1	rate					783:786	specific growth rate	767:786	specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups	767:840	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	4	92	from	Diet	817:820	arg1	WGR					758:760	WGR	758:760	WGR	758:760	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	4	92	from	Diet	817:820	arg1	rate					752:755	the weight gain rate	736:755	the weight gain rate (WGR)	736:761	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	7	93	theme	glutathione	1383:1393	arg1	GPx					1407:1409	GPx	1407:1409	GPx	1407:1409	The total antioxidant capacity (T-AOC), catalase (CAT) and glutathione peroxidase (GPx) activities in the hepatopancreas and hemolymph of crayfish fed Diet 5 and Diet 6 were significantly higher than those in the control group.
34737131	7	93	theme	glutathione	1383:1393	arg1	peroxidase					1395:1404	glutathione peroxidase	1383:1404	glutathione peroxidase (GPx)	1383:1410	The total antioxidant capacity (T-AOC), catalase (CAT) and glutathione peroxidase (GPx) activities in the hepatopancreas and hemolymph of crayfish fed Diet 5 and Diet 6 were significantly higher than those in the control group.
34737131	10	94	theme	factor	1964:1969	arg1	levels					1904:1909	the expression levels	1889:1909	the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA	1889:2103	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	7	95	from	capacity	1346:1353	arg1	hepatopancreas					1430:1443	the hepatopancreas	1426:1443	the hepatopancreas	1426:1443	The total antioxidant capacity (T-AOC), catalase (CAT) and glutathione peroxidase (GPx) activities in the hepatopancreas and hemolymph of crayfish fed Diet 5 and Diet 6 were significantly higher than those in the control group.
34737131	4	96	theme	control	888:894	arg1	group					896:900	the control group	884:900	the control group (Diet 1)	884:909	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	4	96	theme	control	888:894	arg1	Diet					903:906	Diet 1	903:908	Diet 1	903:908	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	2	97	theme	crayfish	459:466	arg1	total					417:421	A total	415:421	A total of 540 (body weight of 0.41 ± 0.05) crayfish	415:466	A total of 540 (body weight of 0.41 ± 0.05) crayfish were randomly divided into six groups for a feeding experiment.
34737131	12	98	theme	claw	2462:2465	arg1	crayfish					2467:2474	juvenile red claw crayfish	2449:2474	juvenile red claw crayfish	2449:2474	These results suggested that supplementation of 5-15 g kg-1 trehalose in feed could significantly improve the growth performance, muscle protein, non-specific immunity and desiccation resistance of juvenile red claw crayfish.
34737131	6	99	theme	dismutase	1072:1080	arg1	activities					1047:1056	The activities	1043:1056	The activities of superoxide dismutase (SOD) and alkaline phosphatase (AKP) in hepatopancreas and hemolymph of crayfish for Diet 4, Diet 5, and Diet 6 groups	1043:1199	The activities of superoxide dismutase (SOD) and alkaline phosphatase (AKP) in hepatopancreas and hemolymph of crayfish for Diet 4, Diet 5, and Diet 6 groups were significantly increased while malondialdehyde (MDA) content was significantly reduced when compared with the control.
34737131	1	100	theme	gene	316:319	arg1	expression					321:330	gene expression	316:330	gene expression	316:330	This study was performed to investigate the effects of dietary trehalose on growth, muscle composition, non-specific immune responses, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	3	101	theme	claw	685:688	arg1	crayfish					690:697	juvenile red claw crayfish	672:697	juvenile red claw crayfish	672:697	Six diets with trehalose levels at 0 (Diet 1), 1 (Diet 2), 2 (Diet 3), 5 (Diet 4), 10 (Diet 5) and 15 (Diet 6) g kg-1 were prepared to feed juvenile red claw crayfish for 8 weeks.
34737131	2	102	theme	0.41 ± 0.05	446:456	arg1	weight					436:441	body weight	431:441	body weight of 0.41 ± 0.05	431:456	A total of 540 (body weight of 0.41 ± 0.05) crayfish were randomly divided into six groups for a feeding experiment.
34737131	3	103	theme	g	643:643	arg1	kg-1					645:648	g kg-1	643:648	g kg-1	643:648	Six diets with trehalose levels at 0 (Diet 1), 1 (Diet 2), 2 (Diet 3), 5 (Diet 4), 10 (Diet 5) and 15 (Diet 6) g kg-1 were prepared to feed juvenile red claw crayfish for 8 weeks.
34737131	0	104	theme	gene	82:85	arg1	expression					87:96	gene expression	82:96	gene expression	82:96	Effects of dietary trehalose on growth, trehalose content, non-specific immunity, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	4	105	dep	Diet	809:812	arg1	groups					835:840	6 groups	833:840	6 groups	833:840	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	3	106	theme	juvenile	672:679	arg1	crayfish					690:697	juvenile red claw crayfish	672:697	juvenile red claw crayfish	672:697	Six diets with trehalose levels at 0 (Diet 1), 1 (Diet 2), 2 (Diet 3), 5 (Diet 4), 10 (Diet 5) and 15 (Diet 6) g kg-1 were prepared to feed juvenile red claw crayfish for 8 weeks.
34737131	10	107	theme	X2	2028:2029	arg1	levels					1904:1909	the expression levels	1889:1909	the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA	1889:2103	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	10	108	theme	5-15 g kg-1	1842:1852	arg1	trehalose					1854:1862	5-15 g kg-1 trehalose	1842:1862	5-15 g kg-1 trehalose	1842:1862	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	3	109	from	0	567:567	arg1	diets					536:540	Six diets	532:540	Six diets with trehalose levels at 0 (Diet 1), 1 (Diet 2), 2 (Diet 3), 5 (Diet 4), 10 (Diet 5) and 15 (Diet 6) g kg-1	532:648	Six diets with trehalose levels at 0 (Diet 1), 1 (Diet 2), 2 (Diet 3), 5 (Diet 4), 10 (Diet 5) and 15 (Diet 6) g kg-1 were prepared to feed juvenile red claw crayfish for 8 weeks.
34737131	4	110	theme	weight	740:745	arg1	WGR					758:760	WGR	758:760	WGR	758:760	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	4	110	theme	weight	740:745	arg1	rate					752:755	the weight gain rate	736:755	the weight gain rate (WGR)	736:761	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	5	111	theme	control	1028:1034	arg1	group					1036:1040	the control group	1024:1040	the control group	1024:1040	Muscle crude protein contents of crayfish fed Diet 4, Diet 5 and Diet 6 were significantly higher than those of the control group.
34737131	10	112	theme	isoform	2020:2026	arg1	Tret1-2					2032:2038	Tret1-2	2032:2038	Tret1-2	2032:2038	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	10	112	theme	isoform	2020:2026	arg1	X2					2028:2029	facilitated trehalose transporter homolog isoform X2	1978:2029	facilitated trehalose transporter homolog isoform X2 (Tret1-2)	1978:2039	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	1	113	theme	juvenile	362:369	arg1	quadricarinatus					397:411	juvenile red claw crayfish (Cherax quadricarinatus)	362:412	juvenile red claw crayfish (Cherax quadricarinatus)	362:412	This study was performed to investigate the effects of dietary trehalose on growth, muscle composition, non-specific immune responses, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	9	114	theme	hepatopancreas	1660:1673	arg1	contents					1699:1706	The hepatopancreas and intestine trehalose contents	1656:1706	The hepatopancreas and intestine trehalose contents of crayfish	1656:1718	The hepatopancreas and intestine trehalose contents of crayfish showed an upward trend with the increase of dietary trehalose levels.
34737131	12	115	theme	desiccation	2423:2433	arg1	resistance					2435:2444	desiccation resistance	2423:2444	desiccation resistance	2423:2444	These results suggested that supplementation of 5-15 g kg-1 trehalose in feed could significantly improve the growth performance, muscle protein, non-specific immunity and desiccation resistance of juvenile red claw crayfish.
34737131	0	116	theme	trehalose	19:27	arg1	Effects					0:6	Effects	0:6	Effects of dietary trehalose on growth, trehalose content, non-specific immunity, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus)	0:178	Effects of dietary trehalose on growth, trehalose content, non-specific immunity, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	6	117	from	activities	1047:1056	arg1	hemolymph					1141:1149	hemolymph	1141:1149	hemolymph	1141:1149	The activities of superoxide dismutase (SOD) and alkaline phosphatase (AKP) in hepatopancreas and hemolymph of crayfish for Diet 4, Diet 5, and Diet 6 groups were significantly increased while malondialdehyde (MDA) content was significantly reduced when compared with the control.
34737131	6	117	from	activities	1047:1056	arg1	hepatopancreas					1122:1135	hepatopancreas	1122:1135	hepatopancreas	1122:1135	The activities of superoxide dismutase (SOD) and alkaline phosphatase (AKP) in hepatopancreas and hemolymph of crayfish for Diet 4, Diet 5, and Diet 6 groups were significantly increased while malondialdehyde (MDA) content was significantly reduced when compared with the control.
34737131	12	118	theme	juvenile	2449:2456	arg1	crayfish					2467:2474	juvenile red claw crayfish	2449:2474	juvenile red claw crayfish	2449:2474	These results suggested that supplementation of 5-15 g kg-1 trehalose in feed could significantly improve the growth performance, muscle protein, non-specific immunity and desiccation resistance of juvenile red claw crayfish.
34737131	9	119	theme	intestine	1679:1687	arg1	contents					1699:1706	The hepatopancreas and intestine trehalose contents	1656:1706	The hepatopancreas and intestine trehalose contents of crayfish	1656:1718	The hepatopancreas and intestine trehalose contents of crayfish showed an upward trend with the increase of dietary trehalose levels.
34737131	6	120	theme	phosphatase	1101:1111	arg1	activities					1047:1056	The activities	1043:1056	The activities of superoxide dismutase (SOD) and alkaline phosphatase (AKP) in hepatopancreas and hemolymph of crayfish for Diet 4, Diet 5, and Diet 6 groups	1043:1199	The activities of superoxide dismutase (SOD) and alkaline phosphatase (AKP) in hepatopancreas and hemolymph of crayfish for Diet 4, Diet 5, and Diet 6 groups were significantly increased while malondialdehyde (MDA) content was significantly reduced when compared with the control.
34737131	10	121	theme	isoform	2079:2085	arg1	mRNA					2100:2103	facilitated trehalose transporter isoform X4 (Tret1-4) mRNA	2045:2103	facilitated trehalose transporter isoform X4 (Tret1-4) mRNA	2045:2103	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	10	122	theme	homolog	2012:2018	arg1	Tret1-2					2032:2038	Tret1-2	2032:2038	Tret1-2	2032:2038	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	10	122	theme	homolog	2012:2018	arg1	X2					2028:2029	facilitated trehalose transporter homolog isoform X2	1978:2029	facilitated trehalose transporter homolog isoform X2 (Tret1-2)	1978:2039	Compared with the control group, supplementation of 5-15 g kg-1 trehalose in the feed up-regulated the expression levels of GPx, C-type lysozyme (C-LZM), antilipolysacchride factor (ALF), facilitated trehalose transporter homolog isoform X2 (Tret1-2) and facilitated trehalose transporter isoform X4 (Tret1-4) mRNA.
34737131	0	123	theme	trehalose	40:48	arg1	content					50:56	trehalose content	40:56	trehalose content	40:56	Effects of dietary trehalose on growth, trehalose content, non-specific immunity, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	1	124	theme	non-specific	285:296	arg1	responses					305:313	non-specific immune responses	285:313	non-specific immune responses	285:313	This study was performed to investigate the effects of dietary trehalose on growth, muscle composition, non-specific immune responses, gene expression and desiccation resistance of juvenile red claw crayfish (Cherax quadricarinatus).
34737131	4	125	theme	growth	776:781	arg1	SGR					789:791	SGR	789:791	SGR	789:791	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	4	125	theme	growth	776:781	arg1	rate					783:786	specific growth rate	767:786	specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups	767:840	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	9	126	theme	crayfish	1711:1718	arg1	contents					1699:1706	The hepatopancreas and intestine trehalose contents	1656:1706	The hepatopancreas and intestine trehalose contents of crayfish	1656:1718	The hepatopancreas and intestine trehalose contents of crayfish showed an upward trend with the increase of dietary trehalose levels.
34737131	12	127	from	supplementation	2280:2294	arg1	feed					2324:2327	feed	2324:2327	feed	2324:2327	These results suggested that supplementation of 5-15 g kg-1 trehalose in feed could significantly improve the growth performance, muscle protein, non-specific immunity and desiccation resistance of juvenile red claw crayfish.
34737131	11	128	theme	survival	2190:2197	arg1	rate					2199:2202	the survival rate	2186:2202	the survival rate of red claw crayfish	2186:2223	In addition, supplementation of 5-15 g kg-1 trehalose in the feed could improve the survival rate of red claw crayfish under desiccation stress.
34737131	5	129	theme	crayfish	945:952	arg1	contents					933:940	Muscle crude protein contents	912:940	Muscle crude protein contents of crayfish fed Diet 4, Diet 5 and Diet 6	912:982	Muscle crude protein contents of crayfish fed Diet 4, Diet 5 and Diet 6 were significantly higher than those of the control group.
34737131	7	130	theme	crayfish	1462:1469	arg1	capacity					1346:1353	The total antioxidant capacity	1324:1353	The total antioxidant capacity (T-AOC)	1324:1361	The total antioxidant capacity (T-AOC), catalase (CAT) and glutathione peroxidase (GPx) activities in the hepatopancreas and hemolymph of crayfish fed Diet 5 and Diet 6 were significantly higher than those in the control group.
34737131	7	130	theme	crayfish	1462:1469	arg1	higher					1512:1517	higher	1512:1517	higher	1512:1517	The total antioxidant capacity (T-AOC), catalase (CAT) and glutathione peroxidase (GPx) activities in the hepatopancreas and hemolymph of crayfish fed Diet 5 and Diet 6 were significantly higher than those in the control group.
34737131	7	130	theme	crayfish	1462:1469	arg1	hemolymph					1449:1457	hemolymph	1449:1457	hemolymph of crayfish fed Diet 5 and Diet 6	1449:1491	The total antioxidant capacity (T-AOC), catalase (CAT) and glutathione peroxidase (GPx) activities in the hepatopancreas and hemolymph of crayfish fed Diet 5 and Diet 6 were significantly higher than those in the control group.
34737131	7	130	theme	crayfish	1462:1469	arg1	activities					1412:1421	catalase (CAT) and glutathione peroxidase (GPx) activities	1364:1421	catalase (CAT) and glutathione peroxidase (GPx) activities in the hepatopancreas	1364:1443	The total antioxidant capacity (T-AOC), catalase (CAT) and glutathione peroxidase (GPx) activities in the hepatopancreas and hemolymph of crayfish fed Diet 5 and Diet 6 were significantly higher than those in the control group.
34737131	7	130	theme	crayfish	1462:1469	arg1	T-AOC					1356:1360	T-AOC	1356:1360	T-AOC	1356:1360	The total antioxidant capacity (T-AOC), catalase (CAT) and glutathione peroxidase (GPx) activities in the hepatopancreas and hemolymph of crayfish fed Diet 5 and Diet 6 were significantly higher than those in the control group.
34737131	7	131	from	activities	1412:1421	arg1	hepatopancreas					1430:1443	the hepatopancreas	1426:1443	the hepatopancreas	1426:1443	The total antioxidant capacity (T-AOC), catalase (CAT) and glutathione peroxidase (GPx) activities in the hepatopancreas and hemolymph of crayfish fed Diet 5 and Diet 6 were significantly higher than those in the control group.
34737131	3	132	with	diets	536:540	arg1	levels					557:562	trehalose levels	547:562	trehalose levels	547:562	Six diets with trehalose levels at 0 (Diet 1), 1 (Diet 2), 2 (Diet 3), 5 (Diet 4), 10 (Diet 5) and 15 (Diet 6) g kg-1 were prepared to feed juvenile red claw crayfish for 8 weeks.
34737131	4	133	from	rate	752:755	arg1	Diet					809:812	Diet 4	809:814	Diet 4	809:814	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	4	133	from	rate	752:755	arg1	Diet					817:820	Diet 5	817:822	Diet 5	817:822	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
34737131	4	133	from	rate	752:755	arg1	Diet					828:831	Diet	828:831	Diet	828:831	The results showed that the weight gain rate (WGR) and specific growth rate (SGR) of crayfish in Diet 4, Diet 5 and Diet 6 groups were significantly improved compared with the control group (Diet 1).
33357916	1	0	theme	synergistic	173:183	arg1	activities					196:205	synergistic biological activities	173:205	synergistic biological activities	173:205	Probiotics and curcumin can exhibit synergistic biological activities on the basis of a gut-brain axis, but are sensitive to environmental conditions, making it a challenge for their co-utilization.
33357916	4	1	theme	curcumin	879:886	arg1	%					874:874	up to 91.3 %	863:874	up to 91.3 % of curcumin and 9.72 log CFU cm-3	863:908	Moreover, it could also largely reduce the chemical degradation of curcumin and increase the survival of LGG during light exposure and long-term storage: up to 91.3 % of curcumin and 9.72 log CFU cm-3 remained present throughout 4 weeks of storage.
33357916	4	1	theme	curcumin	879:886	arg1	CFU					901:903	9.72 log CFU cm-3	892:908	9.72 log CFU cm-3	892:908	Moreover, it could also largely reduce the chemical degradation of curcumin and increase the survival of LGG during light exposure and long-term storage: up to 91.3 % of curcumin and 9.72 log CFU cm-3 remained present throughout 4 weeks of storage.
33357916	4	1	theme	curcumin	879:886	arg1	curcumin					879:886	curcumin	879:886	curcumin	879:886	Moreover, it could also largely reduce the chemical degradation of curcumin and increase the survival of LGG during light exposure and long-term storage: up to 91.3 % of curcumin and 9.72 log CFU cm-3 remained present throughout 4 weeks of storage.
33357916	1	2	theme	biological	185:194	arg1	activities					196:205	synergistic biological activities	173:205	synergistic biological activities	173:205	Probiotics and curcumin can exhibit synergistic biological activities on the basis of a gut-brain axis, but are sensitive to environmental conditions, making it a challenge for their co-utilization.
33357916	1	3	theme	environmental	262:274	arg1	conditions					276:285	environmental conditions	262:285	environmental conditions	262:285	Probiotics and curcumin can exhibit synergistic biological activities on the basis of a gut-brain axis, but are sensitive to environmental conditions, making it a challenge for their co-utilization.
33357916	0	4	theme	probiotics	112:121	arg1	co-delivery					97:107	co-delivery	97:107	co-delivery of probiotics and curcumin	97:134	Assembly of propylene glycol alginate/β-lactoglobulin composite hydrogels induced by ethanol for co-delivery of probiotics and curcumin.
33357916	4	5	theme	log	897:899	arg1	CFU					901:903	9.72 log CFU cm-3	892:908	9.72 log CFU cm-3	892:908	Moreover, it could also largely reduce the chemical degradation of curcumin and increase the survival of LGG during light exposure and long-term storage: up to 91.3 % of curcumin and 9.72 log CFU cm-3 remained present throughout 4 weeks of storage.
33357916	3	6	theme	composite	565:573	arg1	effective					588:596	effective	588:596	effective	588:596	The composite hydrogel was effective at sustaining the release of curcumin and protecting LGG cells in simulated gastrointestinal tract conditions.
33357916	3	6	theme	composite	565:573	arg1	hydrogel					575:582	The composite hydrogel	561:582	The composite hydrogel	561:582	The composite hydrogel was effective at sustaining the release of curcumin and protecting LGG cells in simulated gastrointestinal tract conditions.
33357916	4	7	dep	91.3	869:872	arg1	to					866:867	to	866:867	to	866:867	Moreover, it could also largely reduce the chemical degradation of curcumin and increase the survival of LGG during light exposure and long-term storage: up to 91.3 % of curcumin and 9.72 log CFU cm-3 remained present throughout 4 weeks of storage.
33357916	2	8	theme	ethanol-induced	535:549	arg1	approach					551:558	an ethanol-induced approach	532:558	an ethanol-induced approach	532:558	To meet the demand for high efficiency and convenience, both probiotics and curcumin were encapsulated within a propylene glycol alginate-based hydrogel delivery system, which was assembled using an ethanol-induced approach.
33357916	4	9	theme	9.72	892:895	arg1	CFU					901:903	9.72 log CFU cm-3	892:908	9.72 log CFU cm-3	892:908	Moreover, it could also largely reduce the chemical degradation of curcumin and increase the survival of LGG during light exposure and long-term storage: up to 91.3 % of curcumin and 9.72 log CFU cm-3 remained present throughout 4 weeks of storage.
33357916	2	10	theme	high	359:362	arg1	efficiency					364:373	high efficiency	359:373	high efficiency	359:373	To meet the demand for high efficiency and convenience, both probiotics and curcumin were encapsulated within a propylene glycol alginate-based hydrogel delivery system, which was assembled using an ethanol-induced approach.
33357916	0	11	theme	curcumin	127:134	arg1	co-delivery					97:107	co-delivery	97:107	co-delivery of probiotics and curcumin	97:134	Assembly of propylene glycol alginate/β-lactoglobulin composite hydrogels induced by ethanol for co-delivery of probiotics and curcumin.
33357916	4	12	theme	LGG	814:816	arg1	survival					802:809	the survival	798:809	the survival of LGG	798:816	Moreover, it could also largely reduce the chemical degradation of curcumin and increase the survival of LGG during light exposure and long-term storage: up to 91.3 % of curcumin and 9.72 log CFU cm-3 remained present throughout 4 weeks of storage.
33357916	5	13	theme	desired	1115:1121	arg1	biocompatibility					1072:1087	remarkable biocompatibility	1061:1087	remarkable biocompatibility	1061:1087	Results in this work demonstrate a low-energy and green approach to assemble a composite hydrogel with remarkable biocompatibility, which is considered as a desired delivery vehicle for co-delivery of probiotics and curcumin.
33357916	5	13	theme	desired	1115:1121	arg1	vehicle					1132:1138	a desired delivery vehicle	1113:1138	a desired delivery vehicle for co-delivery of probiotics and curcumin	1113:1181	Results in this work demonstrate a low-energy and green approach to assemble a composite hydrogel with remarkable biocompatibility, which is considered as a desired delivery vehicle for co-delivery of probiotics and curcumin.
33357916	5	14	with	hydrogel	1047:1054	arg1	biocompatibility					1072:1087	remarkable biocompatibility	1061:1087	remarkable biocompatibility	1061:1087	Results in this work demonstrate a low-energy and green approach to assemble a composite hydrogel with remarkable biocompatibility, which is considered as a desired delivery vehicle for co-delivery of probiotics and curcumin.
33357916	5	14	with	hydrogel	1047:1054	arg1	vehicle					1132:1138	a desired delivery vehicle	1113:1138	a desired delivery vehicle for co-delivery of probiotics and curcumin	1113:1181	Results in this work demonstrate a low-energy and green approach to assemble a composite hydrogel with remarkable biocompatibility, which is considered as a desired delivery vehicle for co-delivery of probiotics and curcumin.
33357916	5	15	theme	delivery	1123:1130	arg1	biocompatibility					1072:1087	remarkable biocompatibility	1061:1087	remarkable biocompatibility	1061:1087	Results in this work demonstrate a low-energy and green approach to assemble a composite hydrogel with remarkable biocompatibility, which is considered as a desired delivery vehicle for co-delivery of probiotics and curcumin.
33357916	5	15	theme	delivery	1123:1130	arg1	vehicle					1132:1138	a desired delivery vehicle	1113:1138	a desired delivery vehicle for co-delivery of probiotics and curcumin	1113:1181	Results in this work demonstrate a low-energy and green approach to assemble a composite hydrogel with remarkable biocompatibility, which is considered as a desired delivery vehicle for co-delivery of probiotics and curcumin.
33357916	4	16	theme	curcumin	776:783	arg1	degradation					761:771	the chemical degradation	748:771	the chemical degradation of curcumin	748:783	Moreover, it could also largely reduce the chemical degradation of curcumin and increase the survival of LGG during light exposure and long-term storage: up to 91.3 % of curcumin and 9.72 log CFU cm-3 remained present throughout 4 weeks of storage.
33357916	3	17	from	cells	655:659	arg1	tract					691:695	simulated gastrointestinal tract conditions	664:706	simulated gastrointestinal tract conditions	664:706	The composite hydrogel was effective at sustaining the release of curcumin and protecting LGG cells in simulated gastrointestinal tract conditions.
33357916	2	18	theme	propylene	448:456	arg1	glycol					458:463	propylene glycol	448:463	propylene glycol	448:463	To meet the demand for high efficiency and convenience, both probiotics and curcumin were encapsulated within a propylene glycol alginate-based hydrogel delivery system, which was assembled using an ethanol-induced approach.
33357916	0	19	theme	glycol	22:27	arg1	Assembly					0:7	Assembly	0:7	Assembly of propylene glycol	0:27	Assembly of propylene glycol alginate/β-lactoglobulin composite hydrogels induced by ethanol for co-delivery of probiotics and curcumin.
33357916	0	20	theme	propylene	12:20	arg1	glycol					22:27	propylene glycol	12:27	propylene glycol	12:27	Assembly of propylene glycol alginate/β-lactoglobulin composite hydrogels induced by ethanol for co-delivery of probiotics and curcumin.
33357916	3	21	theme	gastrointestinal	674:689	arg1	tract					691:695	simulated gastrointestinal tract conditions	664:706	simulated gastrointestinal tract conditions	664:706	The composite hydrogel was effective at sustaining the release of curcumin and protecting LGG cells in simulated gastrointestinal tract conditions.
33357916	0	22	theme	composite	54:62	arg1	hydrogels					64:72	composite hydrogels	54:72	composite hydrogels induced by ethanol for co-delivery of probiotics and curcumin	54:134	Assembly of propylene glycol alginate/β-lactoglobulin composite hydrogels induced by ethanol for co-delivery of probiotics and curcumin.
33357916	4	23	theme	storage	949:955	arg1	weeks					940:944	4 weeks	938:944	4 weeks of storage	938:955	Moreover, it could also largely reduce the chemical degradation of curcumin and increase the survival of LGG during light exposure and long-term storage: up to 91.3 % of curcumin and 9.72 log CFU cm-3 remained present throughout 4 weeks of storage.
33357916	1	24	theme	gut-brain	225:233	arg1	axis					235:238	a gut-brain axis	223:238	a gut-brain axis	223:238	Probiotics and curcumin can exhibit synergistic biological activities on the basis of a gut-brain axis, but are sensitive to environmental conditions, making it a challenge for their co-utilization.
33357916	2	25	theme	delivery	489:496	arg1	system					498:503	a propylene glycol alginate-based hydrogel delivery system	446:503	a propylene glycol alginate-based hydrogel delivery system	446:503	To meet the demand for high efficiency and convenience, both probiotics and curcumin were encapsulated within a propylene glycol alginate-based hydrogel delivery system, which was assembled using an ethanol-induced approach.
33357916	5	26	theme	probiotics	1159:1168	arg1	co-delivery					1144:1154	co-delivery	1144:1154	co-delivery of probiotics and curcumin	1144:1181	Results in this work demonstrate a low-energy and green approach to assemble a composite hydrogel with remarkable biocompatibility, which is considered as a desired delivery vehicle for co-delivery of probiotics and curcumin.
33357916	3	27	theme	curcumin	627:634	arg1	release					616:622	the release	612:622	the release of curcumin	612:634	The composite hydrogel was effective at sustaining the release of curcumin and protecting LGG cells in simulated gastrointestinal tract conditions.
33357916	1	28	theme	axis	235:238	arg1	basis					214:218	the basis	210:218	the basis of a gut-brain axis	210:238	Probiotics and curcumin can exhibit synergistic biological activities on the basis of a gut-brain axis, but are sensitive to environmental conditions, making it a challenge for their co-utilization.
33357916	2	29	theme	hydrogel	480:487	arg1	system					498:503	a propylene glycol alginate-based hydrogel delivery system	446:503	a propylene glycol alginate-based hydrogel delivery system	446:503	To meet the demand for high efficiency and convenience, both probiotics and curcumin were encapsulated within a propylene glycol alginate-based hydrogel delivery system, which was assembled using an ethanol-induced approach.
33357916	3	30	theme	simulated	664:672	arg1	tract					691:695	simulated gastrointestinal tract conditions	664:706	simulated gastrointestinal tract conditions	664:706	The composite hydrogel was effective at sustaining the release of curcumin and protecting LGG cells in simulated gastrointestinal tract conditions.
33357916	2	31	theme	alginate-based	465:478	arg1	system					498:503	a propylene glycol alginate-based hydrogel delivery system	446:503	a propylene glycol alginate-based hydrogel delivery system	446:503	To meet the demand for high efficiency and convenience, both probiotics and curcumin were encapsulated within a propylene glycol alginate-based hydrogel delivery system, which was assembled using an ethanol-induced approach.
33357916	5	32	theme	curcumin	1174:1181	arg1	co-delivery					1144:1154	co-delivery	1144:1154	co-delivery of probiotics and curcumin	1144:1181	Results in this work demonstrate a low-energy and green approach to assemble a composite hydrogel with remarkable biocompatibility, which is considered as a desired delivery vehicle for co-delivery of probiotics and curcumin.
33357916	4	33	theme	CFU	901:903	arg1	%					874:874	up to 91.3 %	863:874	up to 91.3 % of curcumin and 9.72 log CFU cm-3	863:908	Moreover, it could also largely reduce the chemical degradation of curcumin and increase the survival of LGG during light exposure and long-term storage: up to 91.3 % of curcumin and 9.72 log CFU cm-3 remained present throughout 4 weeks of storage.
33357916	4	33	theme	CFU	901:903	arg1	CFU					901:903	9.72 log CFU cm-3	892:908	9.72 log CFU cm-3	892:908	Moreover, it could also largely reduce the chemical degradation of curcumin and increase the survival of LGG during light exposure and long-term storage: up to 91.3 % of curcumin and 9.72 log CFU cm-3 remained present throughout 4 weeks of storage.
33357916	4	33	theme	CFU	901:903	arg1	curcumin					879:886	curcumin	879:886	curcumin	879:886	Moreover, it could also largely reduce the chemical degradation of curcumin and increase the survival of LGG during light exposure and long-term storage: up to 91.3 % of curcumin and 9.72 log CFU cm-3 remained present throughout 4 weeks of storage.
33357916	5	34	theme	composite	1037:1045	arg1	hydrogel					1047:1054	a composite hydrogel	1035:1054	a composite hydrogel with remarkable biocompatibility, which is considered as a desired delivery vehicle for co-delivery of probiotics and curcumin	1035:1181	Results in this work demonstrate a low-energy and green approach to assemble a composite hydrogel with remarkable biocompatibility, which is considered as a desired delivery vehicle for co-delivery of probiotics and curcumin.
33357916	5	35	from	Results	958:964	arg1	work					974:977	this work	969:977	this work	969:977	Results in this work demonstrate a low-energy and green approach to assemble a composite hydrogel with remarkable biocompatibility, which is considered as a desired delivery vehicle for co-delivery of probiotics and curcumin.
33357916	4	36	theme	light	825:829	arg1	exposure					831:838	light exposure	825:838	light exposure	825:838	Moreover, it could also largely reduce the chemical degradation of curcumin and increase the survival of LGG during light exposure and long-term storage: up to 91.3 % of curcumin and 9.72 log CFU cm-3 remained present throughout 4 weeks of storage.
33357916	3	37	theme	LGG	651:653	arg1	cells					655:659	LGG cells	651:659	LGG cells in simulated gastrointestinal tract conditions	651:706	The composite hydrogel was effective at sustaining the release of curcumin and protecting LGG cells in simulated gastrointestinal tract conditions.
33357916	4	38	theme	long-term	844:852	arg1	storage					854:860	long-term storage	844:860	long-term storage	844:860	Moreover, it could also largely reduce the chemical degradation of curcumin and increase the survival of LGG during light exposure and long-term storage: up to 91.3 % of curcumin and 9.72 log CFU cm-3 remained present throughout 4 weeks of storage.
33357916	5	39	theme	remarkable	1061:1070	arg1	biocompatibility					1072:1087	remarkable biocompatibility	1061:1087	remarkable biocompatibility	1061:1087	Results in this work demonstrate a low-energy and green approach to assemble a composite hydrogel with remarkable biocompatibility, which is considered as a desired delivery vehicle for co-delivery of probiotics and curcumin.
33357916	5	39	theme	remarkable	1061:1070	arg1	vehicle					1132:1138	a desired delivery vehicle	1113:1138	a desired delivery vehicle for co-delivery of probiotics and curcumin	1113:1181	Results in this work demonstrate a low-energy and green approach to assemble a composite hydrogel with remarkable biocompatibility, which is considered as a desired delivery vehicle for co-delivery of probiotics and curcumin.
33357916	4	40	theme	chemical	752:759	arg1	degradation					761:771	the chemical degradation	748:771	the chemical degradation of curcumin	748:783	Moreover, it could also largely reduce the chemical degradation of curcumin and increase the survival of LGG during light exposure and long-term storage: up to 91.3 % of curcumin and 9.72 log CFU cm-3 remained present throughout 4 weeks of storage.
33357916	5	41	theme	green	1008:1012	arg1	approach					1014:1021	a low-energy and green approach	991:1021	a low-energy and green approach to assemble a composite hydrogel with remarkable biocompatibility, which is considered as a desired delivery vehicle for co-delivery of probiotics and curcumin	991:1181	Results in this work demonstrate a low-energy and green approach to assemble a composite hydrogel with remarkable biocompatibility, which is considered as a desired delivery vehicle for co-delivery of probiotics and curcumin.
33357916	5	42	theme	low-energy	993:1002	arg1	approach					1014:1021	a low-energy and green approach	991:1021	a low-energy and green approach to assemble a composite hydrogel with remarkable biocompatibility, which is considered as a desired delivery vehicle for co-delivery of probiotics and curcumin	991:1181	Results in this work demonstrate a low-energy and green approach to assemble a composite hydrogel with remarkable biocompatibility, which is considered as a desired delivery vehicle for co-delivery of probiotics and curcumin.
33357916	3	43	dep	tract	691:695	arg1	conditions					697:706	conditions	697:706	simulated gastrointestinal tract conditions	664:706	The composite hydrogel was effective at sustaining the release of curcumin and protecting LGG cells in simulated gastrointestinal tract conditions.
34819075	7	0	dep	activity	871:878	arg1	the					867:869	the	867:869	the	867:869	Different types of essential oils reduce inflammation acting similarly by modulating the activity and action of the NFκB signalling pathway, which is the major regulator of the transcription of pro-inflammatory cytokines.
34819075	17	1	theme	anti-inflammatory	2746:2762	arg1	treatment					2764:2772	an anti-inflammatory treatment	2743:2772	an anti-inflammatory treatment	2743:2772	This supports the relevance of the collection of the lavender flowers from early blooming period for essential oil production and for the utilization as an anti-inflammatory treatment.
34819075	10	2	theme	cytokines	1470:1478	arg1	levels					1438:1443	The mRNA and protein levels	1417:1443	levels	1438:1443	The mRNA and protein levels of four pro-inflammatory cytokines, IL-6, IL-1β, IL-8 and TNFα were determined by Real Time PCR and ELISA measurements.
34819075	9	3	from	effects	1305:1311	arg1	inflammation					1403:1414	P. aeruginosa LPS stimulated inflammation	1374:1414	P. aeruginosa LPS stimulated inflammation	1374:1414	The experiments were carried out on THP-1 human monocyte/macrophage cell line as in vitro cell culture model for monitoring the effects of lavender essential oils and the main compound linalool on P. aeruginosa LPS stimulated inflammation.
34819075	16	4	theme	pro-inflammatory	2521:2536	arg1	TNFα					2569:2572	TNFα	2569:2572	TNFα of THP-1 cells	2569:2587	CONCLUSION Based on our results it has been proven that lavender essential oil extracted at the beginning of flowering period is a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells.
34819075	16	4	theme	pro-inflammatory	2521:2536	arg1	IL-β					2560:2563	IL-β	2560:2563	IL-β	2560:2563	CONCLUSION Based on our results it has been proven that lavender essential oil extracted at the beginning of flowering period is a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells.
34819075	16	4	theme	pro-inflammatory	2521:2536	arg1	cytokines					2538:2546	four pro-inflammatory cytokines	2516:2546	four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells	2516:2587	CONCLUSION Based on our results it has been proven that lavender essential oil extracted at the beginning of flowering period is a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells.
34819075	16	4	theme	pro-inflammatory	2521:2536	arg1	IL-8					2554:2557	IL-8	2554:2557	IL-8	2554:2557	CONCLUSION Based on our results it has been proven that lavender essential oil extracted at the beginning of flowering period is a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells.
34819075	16	4	theme	pro-inflammatory	2521:2536	arg1	IL-6					2548:2551	IL-6	2548:2551	IL-6	2548:2551	CONCLUSION Based on our results it has been proven that lavender essential oil extracted at the beginning of flowering period is a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells.
34819075	4	5	theme	antibacterial	435:447	arg1	activities					449:458	antifungal and antibacterial activities	420:458	antifungal and antibacterial activities	420:458	Furthermore, it possesses antifungal and antibacterial activities.
34819075	10	6	theme	mRNA	1421:1424	arg1	levels					1438:1443	The mRNA and protein levels	1417:1443	levels	1438:1443	The mRNA and protein levels of four pro-inflammatory cytokines, IL-6, IL-1β, IL-8 and TNFα were determined by Real Time PCR and ELISA measurements.
34819075	9	7	theme	in	1258:1259	arg1	model					1280:1284	in vitro cell culture model	1258:1284	in vitro cell culture model for monitoring the effects of lavender essential oils and the main compound linalool on P. aeruginosa LPS stimulated inflammation	1258:1414	The experiments were carried out on THP-1 human monocyte/macrophage cell line as in vitro cell culture model for monitoring the effects of lavender essential oils and the main compound linalool on P. aeruginosa LPS stimulated inflammation.
34819075	9	7	theme	in	1258:1259	arg1	experiments					1181:1191	The experiments	1177:1191	The experiments	1177:1191	The experiments were carried out on THP-1 human monocyte/macrophage cell line as in vitro cell culture model for monitoring the effects of lavender essential oils and the main compound linalool on P. aeruginosa LPS stimulated inflammation.
34819075	7	8	theme	Different	782:790	arg1	types					792:796	Different types	782:796	Different types of essential oils	782:814	Different types of essential oils reduce inflammation acting similarly by modulating the activity and action of the NFκB signalling pathway, which is the major regulator of the transcription of pro-inflammatory cytokines.
34819075	9	9	theme	cell	1267:1270	arg1	model					1280:1284	in vitro cell culture model	1258:1284	in vitro cell culture model for monitoring the effects of lavender essential oils and the main compound linalool on P. aeruginosa LPS stimulated inflammation	1258:1414	The experiments were carried out on THP-1 human monocyte/macrophage cell line as in vitro cell culture model for monitoring the effects of lavender essential oils and the main compound linalool on P. aeruginosa LPS stimulated inflammation.
34819075	9	9	theme	cell	1267:1270	arg1	experiments					1181:1191	The experiments	1177:1191	The experiments	1177:1191	The experiments were carried out on THP-1 human monocyte/macrophage cell line as in vitro cell culture model for monitoring the effects of lavender essential oils and the main compound linalool on P. aeruginosa LPS stimulated inflammation.
34819075	7	10	theme	pathway	914:920	arg1	activity					871:878	activity	871:878	activity	871:878	Different types of essential oils reduce inflammation acting similarly by modulating the activity and action of the NFκB signalling pathway, which is the major regulator of the transcription of pro-inflammatory cytokines.
34819075	7	10	theme	pathway	914:920	arg1	action					884:889	action	884:889	action	884:889	Different types of essential oils reduce inflammation acting similarly by modulating the activity and action of the NFκB signalling pathway, which is the major regulator of the transcription of pro-inflammatory cytokines.
34819075	3	11	used	used	309:312	arg2	oil					295:297	Lavender essential oil	276:297	Lavender essential oil	276:297	Lavender essential oil is mainly used in aromatherapy, but it has several pharmacological and therapeutic properties.
34819075	3	12	theme	Lavender	276:283	arg1	oil					295:297	Lavender essential oil	276:297	Lavender essential oil	276:297	Lavender essential oil is mainly used in aromatherapy, but it has several pharmacological and therapeutic properties.
34819075	6	13	theme	period	660:665	arg1	stages					640:645	the different stages	626:645	the different stages of flowering period	626:665	The constitution of the essential oils extracted from the different stages of flowering period varies, which makes it plausible that the collection time of the flowers influences the anti-inflammatory effects.
34819075	16	14	theme	flowering	2457:2465	arg1	period					2467:2472	flowering period	2457:2472	flowering period	2457:2472	CONCLUSION Based on our results it has been proven that lavender essential oil extracted at the beginning of flowering period is a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells.
34819075	7	15	theme	NFκB	898:901	arg1	regulator					942:950	the major regulator	932:950	the major regulator of the transcription of pro-inflammatory cytokines	932:1001	Different types of essential oils reduce inflammation acting similarly by modulating the activity and action of the NFκB signalling pathway, which is the major regulator of the transcription of pro-inflammatory cytokines.
34819075	7	15	theme	NFκB	898:901	arg1	pathway					914:920	the NFκB signalling pathway	894:920	the NFκB signalling pathway	894:920	Different types of essential oils reduce inflammation acting similarly by modulating the activity and action of the NFκB signalling pathway, which is the major regulator of the transcription of pro-inflammatory cytokines.
34819075	7	16	theme	cytokines	993:1001	arg1	transcription					959:971	the transcription	955:971	the transcription of pro-inflammatory cytokines	955:1001	Different types of essential oils reduce inflammation acting similarly by modulating the activity and action of the NFκB signalling pathway, which is the major regulator of the transcription of pro-inflammatory cytokines.
34819075	17	17	theme	oil	2701:2703	arg1	production					2705:2714	essential oil production	2691:2714	essential oil production	2691:2714	This supports the relevance of the collection of the lavender flowers from early blooming period for essential oil production and for the utilization as an anti-inflammatory treatment.
34819075	4	18	theme	antifungal	420:429	arg1	activities					449:458	antifungal and antibacterial activities	420:458	antifungal and antibacterial activities	420:458	Furthermore, it possesses antifungal and antibacterial activities.
34819075	11	19	theme	oils	1590:1593	arg1	effects					1569:1575	The effects	1565:1575	The effects of essential oils	1565:1593	The effects of essential oils were compared to the response to two NFκB inhibitors, luteolin and ACHP.
34819075	13	20	theme	IL-1β	1886:1890	arg1	oil					1901:1903	IL-1β lavender oil	1886:1903	IL-1β lavender oil	1886:1903	In case of IL-8 and IL-1β lavender oil showed stronger effect compared to linalool and both of them acted similarly to NFκB inhibitors.
34819075	6	21	theme	different	630:638	arg1	stages					640:645	the different stages	626:645	the different stages of flowering period	626:665	The constitution of the essential oils extracted from the different stages of flowering period varies, which makes it plausible that the collection time of the flowers influences the anti-inflammatory effects.
34819075	15	22	theme	IL-1β	2239:2243	arg1	expression					2259:2268	IL-6, IL-1β and IL-8 mRNA expression	2233:2268	IL-6, IL-1β and IL-8 mRNA expression	2233:2268	Although lavender essential oil/end of flowering period decreased IL-6, IL-1β and IL-8 mRNA expression in case of LPS pretreatment, it was not capable to reduce cytokine secretion.
34819075	17	23	theme	flowers	2652:2658	arg1	collection					2625:2634	the collection	2621:2634	the collection of the lavender flowers from early blooming period	2621:2685	This supports the relevance of the collection of the lavender flowers from early blooming period for essential oil production and for the utilization as an anti-inflammatory treatment.
34819075	9	24	theme	lavender	1316:1323	arg1	oils					1335:1338	lavender essential oils	1316:1338	lavender essential oils	1316:1338	The experiments were carried out on THP-1 human monocyte/macrophage cell line as in vitro cell culture model for monitoring the effects of lavender essential oils and the main compound linalool on P. aeruginosa LPS stimulated inflammation.
34819075	2	25	theme	nosocomial	241:250	arg1	infections					264:273	nosocomial respiratory infections	241:273	nosocomial respiratory infections	241:273	BACKGROUND Pseudomonas aeruginosa is the most common Gram-negative bacterium associated with nosocomial respiratory infections.
34819075	9	26	dep	in	1258:1259	arg1	vitro					1261:1265	vitro	1261:1265	vitro	1261:1265	The experiments were carried out on THP-1 human monocyte/macrophage cell line as in vitro cell culture model for monitoring the effects of lavender essential oils and the main compound linalool on P. aeruginosa LPS stimulated inflammation.
34819075	12	27	theme	pro-inflammatory	1801:1816	arg1	production					1827:1836	pro-inflammatory cytokine production	1801:1836	pro-inflammatory cytokine production	1801:1836	RESULTS Linalool and lavender essential oil extracted from plants at the beginning of flowering period were successful in decreasing pro-inflammatory cytokine production following LPS pretreatment.
34819075	17	28	theme	early	2665:2669	arg1	period					2680:2685	early blooming period	2665:2685	early blooming period	2665:2685	This supports the relevance of the collection of the lavender flowers from early blooming period for essential oil production and for the utilization as an anti-inflammatory treatment.
34819075	9	29	theme	oils	1335:1338	arg1	effects					1305:1311	the effects	1301:1311	the effects of lavender essential oils and the main compound linalool on P. aeruginosa LPS stimulated inflammation	1301:1414	The experiments were carried out on THP-1 human monocyte/macrophage cell line as in vitro cell culture model for monitoring the effects of lavender essential oils and the main compound linalool on P. aeruginosa LPS stimulated inflammation.
34819075	12	30	theme	period	1764:1769	arg1	beginning					1741:1749	the beginning	1737:1749	the beginning of flowering period	1737:1769	RESULTS Linalool and lavender essential oil extracted from plants at the beginning of flowering period were successful in decreasing pro-inflammatory cytokine production following LPS pretreatment.
34819075	7	31	theme	major	936:940	arg1	regulator					942:950	the major regulator	932:950	the major regulator of the transcription of pro-inflammatory cytokines	932:1001	Different types of essential oils reduce inflammation acting similarly by modulating the activity and action of the NFκB signalling pathway, which is the major regulator of the transcription of pro-inflammatory cytokines.
34819075	7	31	theme	major	936:940	arg1	pathway					914:920	the NFκB signalling pathway	894:920	the NFκB signalling pathway	894:920	Different types of essential oils reduce inflammation acting similarly by modulating the activity and action of the NFκB signalling pathway, which is the major regulator of the transcription of pro-inflammatory cytokines.
34819075	3	32	theme	several	342:348	arg1	properties					382:391	several pharmacological and therapeutic properties	342:391	several pharmacological and therapeutic properties	342:391	Lavender essential oil is mainly used in aromatherapy, but it has several pharmacological and therapeutic properties.
34819075	17	33	theme	essential	2691:2699	arg1	oil					2701:2703	essential oil	2691:2703	essential oil production	2691:2714	This supports the relevance of the collection of the lavender flowers from early blooming period for essential oil production and for the utilization as an anti-inflammatory treatment.
34819075	8	34	dep	METHODS	1004:1010	arg1	harvested					1114:1122	harvested	1114:1122	were harvested at the beginning and at the end of flowering period	1109:1174	METHODS Lavender essential oils were distilled from lavender plant cultivated in Hungary and the flowers were harvested at the beginning and at the end of flowering period.
34819075	8	34	dep	METHODS	1004:1010	arg1	distilled					1041:1049	distilled	1041:1049	were distilled from lavender plant cultivated in Hungary	1036:1091	METHODS Lavender essential oils were distilled from lavender plant cultivated in Hungary and the flowers were harvested at the beginning and at the end of flowering period.
34819075	9	35	theme	compound	1353:1360	arg1	linalool					1362:1369	the main compound linalool	1344:1369	the main compound linalool	1344:1369	The experiments were carried out on THP-1 human monocyte/macrophage cell line as in vitro cell culture model for monitoring the effects of lavender essential oils and the main compound linalool on P. aeruginosa LPS stimulated inflammation.
34819075	15	36	theme	LPS	2281:2283	arg1	pretreatment					2285:2296	LPS pretreatment	2281:2296	LPS pretreatment	2281:2296	Although lavender essential oil/end of flowering period decreased IL-6, IL-1β and IL-8 mRNA expression in case of LPS pretreatment, it was not capable to reduce cytokine secretion.
34819075	14	37	theme	essential	2043:2051	arg1	oil/beginning					2053:2065	lavender essential oil/beginning	2034:2065	lavender essential oil/beginning of flowering period	2034:2085	Pretreatments with linalool and lavender essential oil/beginning of flowering period prevented pro-inflammatory cytokine production compared to LPS treatment alone.
34819075	16	38	dep	cytokines	2538:2546	arg1	TNFα					2569:2572	TNFα	2569:2572	TNFα of THP-1 cells	2569:2587	CONCLUSION Based on our results it has been proven that lavender essential oil extracted at the beginning of flowering period is a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells.
34819075	16	38	dep	cytokines	2538:2546	arg1	IL-β					2560:2563	IL-β	2560:2563	IL-β	2560:2563	CONCLUSION Based on our results it has been proven that lavender essential oil extracted at the beginning of flowering period is a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells.
34819075	16	38	dep	cytokines	2538:2546	arg1	cytokines					2538:2546	four pro-inflammatory cytokines	2516:2546	four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells	2516:2587	CONCLUSION Based on our results it has been proven that lavender essential oil extracted at the beginning of flowering period is a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells.
34819075	16	38	dep	cytokines	2538:2546	arg1	IL-8					2554:2557	IL-8	2554:2557	IL-8	2554:2557	CONCLUSION Based on our results it has been proven that lavender essential oil extracted at the beginning of flowering period is a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells.
34819075	16	38	dep	cytokines	2538:2546	arg1	IL-6					2548:2551	IL-6	2548:2551	IL-6	2548:2551	CONCLUSION Based on our results it has been proven that lavender essential oil extracted at the beginning of flowering period is a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells.
34819075	14	39	theme	pro-inflammatory	2097:2112	arg1	production					2123:2132	pro-inflammatory cytokine production	2097:2132	pro-inflammatory cytokine production	2097:2132	Pretreatments with linalool and lavender essential oil/beginning of flowering period prevented pro-inflammatory cytokine production compared to LPS treatment alone.
34819075	12	40	theme	essential	1698:1706	arg1	oil					1708:1710	lavender essential oil	1689:1710	lavender essential oil	1689:1710	RESULTS Linalool and lavender essential oil extracted from plants at the beginning of flowering period were successful in decreasing pro-inflammatory cytokine production following LPS pretreatment.
34819075	12	40	theme	essential	1698:1706	arg1	RESULTS					1668:1674	RESULTS Linalool and lavender essential oil	1668:1710	RESULTS Linalool and lavender essential oil extracted from plants at the beginning of flowering period	1668:1769	RESULTS Linalool and lavender essential oil extracted from plants at the beginning of flowering period were successful in decreasing pro-inflammatory cytokine production following LPS pretreatment.
34819075	17	41	theme	collection	2625:2634	arg1	relevance					2608:2616	the relevance	2604:2616	the relevance of the collection of the lavender flowers from early blooming period for essential oil production and for the utilization as an anti-inflammatory treatment	2604:2772	This supports the relevance of the collection of the lavender flowers from early blooming period for essential oil production and for the utilization as an anti-inflammatory treatment.
34819075	10	42	theme	Real	1527:1530	arg1	PCR					1537:1539	Real Time PCR	1527:1539	Real Time PCR	1527:1539	The mRNA and protein levels of four pro-inflammatory cytokines, IL-6, IL-1β, IL-8 and TNFα were determined by Real Time PCR and ELISA measurements.
34819075	6	43	theme	anti-inflammatory	755:771	arg1	effects					773:779	the anti-inflammatory effects	751:779	the anti-inflammatory effects	751:779	The constitution of the essential oils extracted from the different stages of flowering period varies, which makes it plausible that the collection time of the flowers influences the anti-inflammatory effects.
34819075	9	44	theme	aeruginosa	1377:1386	arg1	inflammation					1403:1414	P. aeruginosa LPS stimulated inflammation	1374:1414	P. aeruginosa LPS stimulated inflammation	1374:1414	The experiments were carried out on THP-1 human monocyte/macrophage cell line as in vitro cell culture model for monitoring the effects of lavender essential oils and the main compound linalool on P. aeruginosa LPS stimulated inflammation.
34819075	14	45	theme	period	2080:2085	arg1	linalool					2021:2028	linalool	2021:2028	linalool	2021:2028	Pretreatments with linalool and lavender essential oil/beginning of flowering period prevented pro-inflammatory cytokine production compared to LPS treatment alone.
34819075	14	45	theme	period	2080:2085	arg1	oil/beginning					2053:2065	lavender essential oil/beginning	2034:2065	lavender essential oil/beginning of flowering period	2034:2085	Pretreatments with linalool and lavender essential oil/beginning of flowering period prevented pro-inflammatory cytokine production compared to LPS treatment alone.
34819075	3	46	contain	has	338:340	arg1	it					335:336	it	335:336	it	335:336	Lavender essential oil is mainly used in aromatherapy, but it has several pharmacological and therapeutic properties.
34819075	3	46	contain	has	338:340	arg2	properties					382:391	several pharmacological and therapeutic properties	342:391	several pharmacological and therapeutic properties	342:391	Lavender essential oil is mainly used in aromatherapy, but it has several pharmacological and therapeutic properties.
34819075	6	47	dep	influences	740:749	arg1	plausible					690:698	plausible	690:698	plausible	690:698	The constitution of the essential oils extracted from the different stages of flowering period varies, which makes it plausible that the collection time of the flowers influences the anti-inflammatory effects.
34819075	0	48	theme	Anti-inflammatory	0:16	arg1	effect					18:23	Anti-inflammatory effect	0:23	Anti-inflammatory effect of lavender (Lavandula angustifolia Mill.)	0:66	Anti-inflammatory effect of lavender (Lavandula angustifolia Mill.)
34819075	0	48	theme	Anti-inflammatory	0:16	arg1	Mill					61:64	Lavandula angustifolia Mill.	38:65	Lavandula angustifolia Mill.	38:65	Anti-inflammatory effect of lavender (Lavandula angustifolia Mill.)
34819075	9	49	theme	stimulated	1392:1401	arg1	inflammation					1403:1414	P. aeruginosa LPS stimulated inflammation	1374:1414	P. aeruginosa LPS stimulated inflammation	1374:1414	The experiments were carried out on THP-1 human monocyte/macrophage cell line as in vitro cell culture model for monitoring the effects of lavender essential oils and the main compound linalool on P. aeruginosa LPS stimulated inflammation.
34819075	1	50	from	phenophases	114:124	arg1	macrophages					135:145	THP-1 macrophages	129:145	THP-1 macrophages	129:145	essential oil prepared during different plant phenophases on THP-1 macrophages.
34819075	17	51	from	period	2680:2685	arg1	collection					2625:2634	the collection	2621:2634	the collection of the lavender flowers from early blooming period	2621:2685	This supports the relevance of the collection of the lavender flowers from early blooming period for essential oil production and for the utilization as an anti-inflammatory treatment.
34819075	17	51	from	period	2680:2685	arg1	flowers					2652:2658	the lavender flowers	2639:2658	the lavender flowers from early blooming period	2639:2685	This supports the relevance of the collection of the lavender flowers from early blooming period for essential oil production and for the utilization as an anti-inflammatory treatment.
34819075	13	52	theme	lavender	1892:1899	arg1	oil					1901:1903	IL-1β lavender oil	1886:1903	IL-1β lavender oil	1886:1903	In case of IL-8 and IL-1β lavender oil showed stronger effect compared to linalool and both of them acted similarly to NFκB inhibitors.
34819075	1	53	theme	plant	108:112	arg1	phenophases					114:124	different plant phenophases	98:124	different plant phenophases on THP-1 macrophages	98:145	essential oil prepared during different plant phenophases on THP-1 macrophages.
34819075	2	54	dep	Pseudomonas	159:169	arg1	aeruginosa					171:180	aeruginosa	171:180	aeruginosa	171:180	BACKGROUND Pseudomonas aeruginosa is the most common Gram-negative bacterium associated with nosocomial respiratory infections.
34819075	15	55	theme	cytokine	2328:2335	arg1	secretion					2337:2345	cytokine secretion	2328:2345	cytokine secretion	2328:2345	Although lavender essential oil/end of flowering period decreased IL-6, IL-1β and IL-8 mRNA expression in case of LPS pretreatment, it was not capable to reduce cytokine secretion.
34819075	15	56	theme	IL-6	2233:2236	arg1	expression					2259:2268	IL-6, IL-1β and IL-8 mRNA expression	2233:2268	IL-6, IL-1β and IL-8 mRNA expression	2233:2268	Although lavender essential oil/end of flowering period decreased IL-6, IL-1β and IL-8 mRNA expression in case of LPS pretreatment, it was not capable to reduce cytokine secretion.
34819075	14	57	theme	LPS	2146:2148	arg1	treatment					2150:2158	LPS treatment	2146:2158	LPS treatment alone	2146:2164	Pretreatments with linalool and lavender essential oil/beginning of flowering period prevented pro-inflammatory cytokine production compared to LPS treatment alone.
34819075	16	58	theme	period	2467:2472	arg1	beginning					2444:2452	the beginning	2440:2452	the beginning of flowering period	2440:2472	CONCLUSION Based on our results it has been proven that lavender essential oil extracted at the beginning of flowering period is a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells.
34819075	0	59	theme	angustifolia	48:59	arg1	effect					18:23	Anti-inflammatory effect	0:23	Anti-inflammatory effect of lavender (Lavandula angustifolia Mill.)	0:66	Anti-inflammatory effect of lavender (Lavandula angustifolia Mill.)
34819075	0	59	theme	angustifolia	48:59	arg1	Mill					61:64	Lavandula angustifolia Mill.	38:65	Lavandula angustifolia Mill.	38:65	Anti-inflammatory effect of lavender (Lavandula angustifolia Mill.)
34819075	8	60	theme	period	1169:1174	arg1	end					1152:1154	the end	1148:1154	the end of flowering period	1148:1174	METHODS Lavender essential oils were distilled from lavender plant cultivated in Hungary and the flowers were harvested at the beginning and at the end of flowering period.
34819075	16	61	theme	cells	2583:2587	arg1	TNFα					2569:2572	TNFα	2569:2572	TNFα of THP-1 cells	2569:2587	CONCLUSION Based on our results it has been proven that lavender essential oil extracted at the beginning of flowering period is a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells.
34819075	16	61	theme	cells	2583:2587	arg1	IL-β					2560:2563	IL-β	2560:2563	IL-β	2560:2563	CONCLUSION Based on our results it has been proven that lavender essential oil extracted at the beginning of flowering period is a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells.
34819075	16	61	theme	cells	2583:2587	arg1	cytokines					2538:2546	four pro-inflammatory cytokines	2516:2546	four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells	2516:2587	CONCLUSION Based on our results it has been proven that lavender essential oil extracted at the beginning of flowering period is a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells.
34819075	16	61	theme	cells	2583:2587	arg1	IL-8					2554:2557	IL-8	2554:2557	IL-8	2554:2557	CONCLUSION Based on our results it has been proven that lavender essential oil extracted at the beginning of flowering period is a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells.
34819075	16	61	theme	cells	2583:2587	arg1	IL-6					2548:2551	IL-6	2548:2551	IL-6	2548:2551	CONCLUSION Based on our results it has been proven that lavender essential oil extracted at the beginning of flowering period is a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells.
34819075	15	62	theme	period	2216:2221	arg1	oil/end					2195:2201	lavender essential oil/end	2176:2201	lavender essential oil/end of flowering period	2176:2221	Although lavender essential oil/end of flowering period decreased IL-6, IL-1β and IL-8 mRNA expression in case of LPS pretreatment, it was not capable to reduce cytokine secretion.
34819075	13	63	theme	NFκB	1985:1988	arg1	inhibitors					1990:1999	NFκB inhibitors	1985:1999	NFκB inhibitors	1985:1999	In case of IL-8 and IL-1β lavender oil showed stronger effect compared to linalool and both of them acted similarly to NFκB inhibitors.
34819075	7	64	theme	pro-inflammatory	976:991	arg1	cytokines					993:1001	pro-inflammatory cytokines	976:1001	pro-inflammatory cytokines	976:1001	Different types of essential oils reduce inflammation acting similarly by modulating the activity and action of the NFκB signalling pathway, which is the major regulator of the transcription of pro-inflammatory cytokines.
34819075	17	65	from	collection	2625:2634	arg1	period					2680:2685	early blooming period	2665:2685	early blooming period	2665:2685	This supports the relevance of the collection of the lavender flowers from early blooming period for essential oil production and for the utilization as an anti-inflammatory treatment.
34819075	16	66	dep	CONCLUSION	2348:2357	arg1	proven					2392:2397	proven	2392:2397	has been proven that lavender essential oil extracted at the beginning of flowering period is a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells	2383:2587	CONCLUSION Based on our results it has been proven that lavender essential oil extracted at the beginning of flowering period is a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells.
34819075	10	67	theme	protein	1430:1436	arg1	levels					1438:1443	The mRNA and protein levels	1417:1443	levels	1438:1443	The mRNA and protein levels of four pro-inflammatory cytokines, IL-6, IL-1β, IL-8 and TNFα were determined by Real Time PCR and ELISA measurements.
34819075	9	68	theme	human	1219:1223	arg1	line					1250:1253	THP-1 human monocyte/macrophage cell line	1213:1253	THP-1 human monocyte/macrophage cell line	1213:1253	The experiments were carried out on THP-1 human monocyte/macrophage cell line as in vitro cell culture model for monitoring the effects of lavender essential oils and the main compound linalool on P. aeruginosa LPS stimulated inflammation.
34819075	7	69	theme	transcription	959:971	arg1	regulator					942:950	the major regulator	932:950	the major regulator of the transcription of pro-inflammatory cytokines	932:1001	Different types of essential oils reduce inflammation acting similarly by modulating the activity and action of the NFκB signalling pathway, which is the major regulator of the transcription of pro-inflammatory cytokines.
34819075	7	69	theme	transcription	959:971	arg1	pathway					914:920	the NFκB signalling pathway	894:920	the NFκB signalling pathway	894:920	Different types of essential oils reduce inflammation acting similarly by modulating the activity and action of the NFκB signalling pathway, which is the major regulator of the transcription of pro-inflammatory cytokines.
34819075	7	70	theme	essential	801:809	arg1	oils					811:814	essential oils	801:814	essential oils	801:814	Different types of essential oils reduce inflammation acting similarly by modulating the activity and action of the NFκB signalling pathway, which is the major regulator of the transcription of pro-inflammatory cytokines.
34819075	15	71	theme	essential	2185:2193	arg1	oil/end					2195:2201	lavender essential oil/end	2176:2201	lavender essential oil/end of flowering period	2176:2221	Although lavender essential oil/end of flowering period decreased IL-6, IL-1β and IL-8 mRNA expression in case of LPS pretreatment, it was not capable to reduce cytokine secretion.
34819075	6	72	theme	essential	596:604	arg1	oils					606:609	the essential oils	592:609	the essential oils extracted from the different stages of flowering period	592:665	The constitution of the essential oils extracted from the different stages of flowering period varies, which makes it plausible that the collection time of the flowers influences the anti-inflammatory effects.
34819075	9	73	theme	cell	1245:1248	arg1	line					1250:1253	THP-1 human monocyte/macrophage cell line	1213:1253	THP-1 human monocyte/macrophage cell line	1213:1253	The experiments were carried out on THP-1 human monocyte/macrophage cell line as in vitro cell culture model for monitoring the effects of lavender essential oils and the main compound linalool on P. aeruginosa LPS stimulated inflammation.
34819075	16	74	theme	cytokines	2538:2546	arg1	synthesis					2503:2511	the synthesis	2499:2511	the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells	2499:2587	CONCLUSION Based on our results it has been proven that lavender essential oil extracted at the beginning of flowering period is a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells.
34819075	8	75	theme	lavender	1056:1063	arg1	plant					1065:1069	lavender plant	1056:1069	lavender plant cultivated in Hungary	1056:1091	METHODS Lavender essential oils were distilled from lavender plant cultivated in Hungary and the flowers were harvested at the beginning and at the end of flowering period.
34819075	10	76	theme	pro-inflammatory	1453:1468	arg1	IL-1β					1487:1491	IL-1β	1487:1491	IL-1β	1487:1491	The mRNA and protein levels of four pro-inflammatory cytokines, IL-6, IL-1β, IL-8 and TNFα were determined by Real Time PCR and ELISA measurements.
34819075	10	76	theme	pro-inflammatory	1453:1468	arg1	TNFα					1503:1506	TNFα	1503:1506	TNFα	1503:1506	The mRNA and protein levels of four pro-inflammatory cytokines, IL-6, IL-1β, IL-8 and TNFα were determined by Real Time PCR and ELISA measurements.
34819075	10	76	theme	pro-inflammatory	1453:1468	arg1	cytokines					1470:1478	four pro-inflammatory cytokines	1448:1478	four pro-inflammatory cytokines	1448:1478	The mRNA and protein levels of four pro-inflammatory cytokines, IL-6, IL-1β, IL-8 and TNFα were determined by Real Time PCR and ELISA measurements.
34819075	10	76	theme	pro-inflammatory	1453:1468	arg1	IL-8					1494:1497	IL-8	1494:1497	IL-8	1494:1497	The mRNA and protein levels of four pro-inflammatory cytokines, IL-6, IL-1β, IL-8 and TNFα were determined by Real Time PCR and ELISA measurements.
34819075	10	76	theme	pro-inflammatory	1453:1468	arg1	IL-6					1481:1484	IL-6	1481:1484	IL-6	1481:1484	The mRNA and protein levels of four pro-inflammatory cytokines, IL-6, IL-1β, IL-8 and TNFα were determined by Real Time PCR and ELISA measurements.
34819075	5	77	theme	essential	495:503	arg1	oils					505:508	essential oils	495:508	essential oils	495:508	The anti-inflammatory activity of essential oils may depend on the composition and the ratio of the compounds.
34819075	2	78	theme	Pseudomonas	159:169	arg1	BACKGROUND					148:157	BACKGROUND	148:157	BACKGROUND Pseudomonas aeruginosa	148:180	BACKGROUND Pseudomonas aeruginosa is the most common Gram-negative bacterium associated with nosocomial respiratory infections.
34819075	2	78	theme	Pseudomonas	159:169	arg1	bacterium					215:223	the most common Gram-negative bacterium	185:223	the most common Gram-negative bacterium associated with nosocomial respiratory infections	185:273	BACKGROUND Pseudomonas aeruginosa is the most common Gram-negative bacterium associated with nosocomial respiratory infections.
34819075	11	79	theme	essential	1580:1588	arg1	oils					1590:1593	essential oils	1580:1593	essential oils	1580:1593	The effects of essential oils were compared to the response to two NFκB inhibitors, luteolin and ACHP.
34819075	9	80	theme	culture	1272:1278	arg1	model					1280:1284	in vitro cell culture model	1258:1284	in vitro cell culture model for monitoring the effects of lavender essential oils and the main compound linalool on P. aeruginosa LPS stimulated inflammation	1258:1414	The experiments were carried out on THP-1 human monocyte/macrophage cell line as in vitro cell culture model for monitoring the effects of lavender essential oils and the main compound linalool on P. aeruginosa LPS stimulated inflammation.
34819075	9	80	theme	culture	1272:1278	arg1	experiments					1181:1191	The experiments	1177:1191	The experiments	1177:1191	The experiments were carried out on THP-1 human monocyte/macrophage cell line as in vitro cell culture model for monitoring the effects of lavender essential oils and the main compound linalool on P. aeruginosa LPS stimulated inflammation.
34819075	5	81	theme	compounds	561:569	arg1	composition					528:538	the composition	524:538	the composition	524:538	The anti-inflammatory activity of essential oils may depend on the composition and the ratio of the compounds.
34819075	5	81	theme	compounds	561:569	arg1	ratio					548:552	the ratio	544:552	the ratio	544:552	The anti-inflammatory activity of essential oils may depend on the composition and the ratio of the compounds.
34819075	15	82	theme	mRNA	2254:2257	arg1	expression					2259:2268	IL-6, IL-1β and IL-8 mRNA expression	2233:2268	IL-6, IL-1β and IL-8 mRNA expression	2233:2268	Although lavender essential oil/end of flowering period decreased IL-6, IL-1β and IL-8 mRNA expression in case of LPS pretreatment, it was not capable to reduce cytokine secretion.
34819075	3	83	theme	essential	285:293	arg1	oil					295:297	Lavender essential oil	276:297	Lavender essential oil	276:297	Lavender essential oil is mainly used in aromatherapy, but it has several pharmacological and therapeutic properties.
34819075	6	84	theme	flowering	650:658	arg1	period					660:665	flowering period	650:665	flowering period	650:665	The constitution of the essential oils extracted from the different stages of flowering period varies, which makes it plausible that the collection time of the flowers influences the anti-inflammatory effects.
34819075	7	85	theme	signalling	903:912	arg1	regulator					942:950	the major regulator	932:950	the major regulator of the transcription of pro-inflammatory cytokines	932:1001	Different types of essential oils reduce inflammation acting similarly by modulating the activity and action of the NFκB signalling pathway, which is the major regulator of the transcription of pro-inflammatory cytokines.
34819075	7	85	theme	signalling	903:912	arg1	pathway					914:920	the NFκB signalling pathway	894:920	the NFκB signalling pathway	894:920	Different types of essential oils reduce inflammation acting similarly by modulating the activity and action of the NFκB signalling pathway, which is the major regulator of the transcription of pro-inflammatory cytokines.
34819075	16	86	theme	potent	2479:2484	arg1	oil					2423:2425	lavender essential oil	2404:2425	lavender essential oil extracted at the beginning of flowering period	2404:2472	CONCLUSION Based on our results it has been proven that lavender essential oil extracted at the beginning of flowering period is a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells.
34819075	16	86	theme	potent	2479:2484	arg1	inhibitor					2486:2494	a potent inhibitor	2477:2494	a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells	2477:2587	CONCLUSION Based on our results it has been proven that lavender essential oil extracted at the beginning of flowering period is a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells.
34819075	16	87	theme	essential	2413:2421	arg1	oil					2423:2425	lavender essential oil	2404:2425	lavender essential oil extracted at the beginning of flowering period	2404:2472	CONCLUSION Based on our results it has been proven that lavender essential oil extracted at the beginning of flowering period is a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells.
34819075	16	87	theme	essential	2413:2421	arg1	inhibitor					2486:2494	a potent inhibitor	2477:2494	a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells	2477:2587	CONCLUSION Based on our results it has been proven that lavender essential oil extracted at the beginning of flowering period is a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells.
34819075	17	88	theme	lavender	2643:2650	arg1	flowers					2652:2658	the lavender flowers	2639:2658	the lavender flowers from early blooming period	2639:2685	This supports the relevance of the collection of the lavender flowers from early blooming period for essential oil production and for the utilization as an anti-inflammatory treatment.
34819075	2	89	theme	respiratory	252:262	arg1	infections					264:273	nosocomial respiratory infections	241:273	nosocomial respiratory infections	241:273	BACKGROUND Pseudomonas aeruginosa is the most common Gram-negative bacterium associated with nosocomial respiratory infections.
34819075	6	90	theme	collection	709:718	arg1	time					720:723	the collection time	705:723	the collection time of the flowers	705:738	The constitution of the essential oils extracted from the different stages of flowering period varies, which makes it plausible that the collection time of the flowers influences the anti-inflammatory effects.
34819075	9	91	theme	essential	1325:1333	arg1	oils					1335:1338	lavender essential oils	1316:1338	lavender essential oils	1316:1338	The experiments were carried out on THP-1 human monocyte/macrophage cell line as in vitro cell culture model for monitoring the effects of lavender essential oils and the main compound linalool on P. aeruginosa LPS stimulated inflammation.
34819075	1	92	theme	THP-1	129:133	arg1	macrophages					135:145	THP-1 macrophages	129:145	THP-1 macrophages	129:145	essential oil prepared during different plant phenophases on THP-1 macrophages.
34819075	17	93	theme	blooming	2671:2678	arg1	period					2680:2685	early blooming period	2665:2685	early blooming period	2665:2685	This supports the relevance of the collection of the lavender flowers from early blooming period for essential oil production and for the utilization as an anti-inflammatory treatment.
34819075	12	94	theme	flowering	1754:1762	arg1	period					1764:1769	flowering period	1754:1769	flowering period	1754:1769	RESULTS Linalool and lavender essential oil extracted from plants at the beginning of flowering period were successful in decreasing pro-inflammatory cytokine production following LPS pretreatment.
34819075	3	95	theme	therapeutic	370:380	arg1	properties					382:391	several pharmacological and therapeutic properties	342:391	several pharmacological and therapeutic properties	342:391	Lavender essential oil is mainly used in aromatherapy, but it has several pharmacological and therapeutic properties.
34819075	9	96	theme	main	1348:1351	arg1	linalool					1362:1369	the main compound linalool	1344:1369	the main compound linalool	1344:1369	The experiments were carried out on THP-1 human monocyte/macrophage cell line as in vitro cell culture model for monitoring the effects of lavender essential oils and the main compound linalool on P. aeruginosa LPS stimulated inflammation.
34819075	3	97	theme	pharmacological	350:364	arg1	properties					382:391	several pharmacological and therapeutic properties	342:391	several pharmacological and therapeutic properties	342:391	Lavender essential oil is mainly used in aromatherapy, but it has several pharmacological and therapeutic properties.
34819075	16	98	theme	lavender	2404:2411	arg1	oil					2423:2425	lavender essential oil	2404:2425	lavender essential oil extracted at the beginning of flowering period	2404:2472	CONCLUSION Based on our results it has been proven that lavender essential oil extracted at the beginning of flowering period is a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells.
34819075	16	98	theme	lavender	2404:2411	arg1	inhibitor					2486:2494	a potent inhibitor	2477:2494	a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells	2477:2587	CONCLUSION Based on our results it has been proven that lavender essential oil extracted at the beginning of flowering period is a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells.
34819075	10	99	dep	PCR	1537:1539	arg1	measurements					1551:1562	measurements	1551:1562	measurements	1551:1562	The mRNA and protein levels of four pro-inflammatory cytokines, IL-6, IL-1β, IL-8 and TNFα were determined by Real Time PCR and ELISA measurements.
34819075	9	100	theme	linalool	1362:1369	arg1	effects					1305:1311	the effects	1301:1311	the effects of lavender essential oils and the main compound linalool on P. aeruginosa LPS stimulated inflammation	1301:1414	The experiments were carried out on THP-1 human monocyte/macrophage cell line as in vitro cell culture model for monitoring the effects of lavender essential oils and the main compound linalool on P. aeruginosa LPS stimulated inflammation.
34819075	14	101	theme	cytokine	2114:2121	arg1	production					2123:2132	pro-inflammatory cytokine production	2097:2132	pro-inflammatory cytokine production	2097:2132	Pretreatments with linalool and lavender essential oil/beginning of flowering period prevented pro-inflammatory cytokine production compared to LPS treatment alone.
34819075	13	102	theme	stronger	1912:1919	arg1	effect					1921:1926	stronger effect	1912:1926	stronger effect	1912:1926	In case of IL-8 and IL-1β lavender oil showed stronger effect compared to linalool and both of them acted similarly to NFκB inhibitors.
34819075	15	103	theme	IL-8	2249:2252	arg1	expression					2259:2268	IL-6, IL-1β and IL-8 mRNA expression	2233:2268	IL-6, IL-1β and IL-8 mRNA expression	2233:2268	Although lavender essential oil/end of flowering period decreased IL-6, IL-1β and IL-8 mRNA expression in case of LPS pretreatment, it was not capable to reduce cytokine secretion.
34819075	1	104	theme	essential	68:76	arg1	oil					78:80	essential oil	68:80	essential oil	68:80	essential oil prepared during different plant phenophases on THP-1 macrophages.
34819075	12	105	theme	lavender	1689:1696	arg1	oil					1708:1710	lavender essential oil	1689:1710	lavender essential oil	1689:1710	RESULTS Linalool and lavender essential oil extracted from plants at the beginning of flowering period were successful in decreasing pro-inflammatory cytokine production following LPS pretreatment.
34819075	12	105	theme	lavender	1689:1696	arg1	RESULTS					1668:1674	RESULTS Linalool and lavender essential oil	1668:1710	RESULTS Linalool and lavender essential oil extracted from plants at the beginning of flowering period	1668:1769	RESULTS Linalool and lavender essential oil extracted from plants at the beginning of flowering period were successful in decreasing pro-inflammatory cytokine production following LPS pretreatment.
34819075	14	106	theme	flowering	2070:2078	arg1	period					2080:2085	flowering period	2070:2085	flowering period	2070:2085	Pretreatments with linalool and lavender essential oil/beginning of flowering period prevented pro-inflammatory cytokine production compared to LPS treatment alone.
34819075	2	107	theme	Gram-negative	201:213	arg1	bacterium					215:223	the most common Gram-negative bacterium	185:223	the most common Gram-negative bacterium associated with nosocomial respiratory infections	185:273	BACKGROUND Pseudomonas aeruginosa is the most common Gram-negative bacterium associated with nosocomial respiratory infections.
34819075	2	107	theme	Gram-negative	201:213	arg1	BACKGROUND					148:157	BACKGROUND	148:157	BACKGROUND Pseudomonas aeruginosa	148:180	BACKGROUND Pseudomonas aeruginosa is the most common Gram-negative bacterium associated with nosocomial respiratory infections.
34819075	6	108	theme	flowers	732:738	arg1	time					720:723	the collection time	705:723	the collection time of the flowers	705:738	The constitution of the essential oils extracted from the different stages of flowering period varies, which makes it plausible that the collection time of the flowers influences the anti-inflammatory effects.
34819075	0	109	theme	lavender	28:35	arg1	effect					18:23	Anti-inflammatory effect	0:23	Anti-inflammatory effect of lavender (Lavandula angustifolia Mill.)	0:66	Anti-inflammatory effect of lavender (Lavandula angustifolia Mill.)
34819075	0	109	theme	lavender	28:35	arg1	Mill					61:64	Lavandula angustifolia Mill.	38:65	Lavandula angustifolia Mill.	38:65	Anti-inflammatory effect of lavender (Lavandula angustifolia Mill.)
34819075	1	110	theme	different	98:106	arg1	phenophases					114:124	different plant phenophases	98:124	different plant phenophases on THP-1 macrophages	98:145	essential oil prepared during different plant phenophases on THP-1 macrophages.
34819075	4	111	contain	possesses	410:418	arg1	it					407:408	it	407:408	it	407:408	Furthermore, it possesses antifungal and antibacterial activities.
34819075	4	111	contain	possesses	410:418	arg2	activities					449:458	antifungal and antibacterial activities	420:458	antifungal and antibacterial activities	420:458	Furthermore, it possesses antifungal and antibacterial activities.
34819075	2	112	theme	common	194:199	arg1	bacterium					215:223	the most common Gram-negative bacterium	185:223	the most common Gram-negative bacterium associated with nosocomial respiratory infections	185:273	BACKGROUND Pseudomonas aeruginosa is the most common Gram-negative bacterium associated with nosocomial respiratory infections.
34819075	2	112	theme	common	194:199	arg1	BACKGROUND					148:157	BACKGROUND	148:157	BACKGROUND Pseudomonas aeruginosa	148:180	BACKGROUND Pseudomonas aeruginosa is the most common Gram-negative bacterium associated with nosocomial respiratory infections.
34819075	0	113	theme	Lavandula	38:46	arg1	effect					18:23	Anti-inflammatory effect	0:23	Anti-inflammatory effect of lavender (Lavandula angustifolia Mill.)	0:66	Anti-inflammatory effect of lavender (Lavandula angustifolia Mill.)
34819075	0	113	theme	Lavandula	38:46	arg1	Mill					61:64	Lavandula angustifolia Mill.	38:65	Lavandula angustifolia Mill.	38:65	Anti-inflammatory effect of lavender (Lavandula angustifolia Mill.)
34819075	8	114	theme	flowering	1159:1167	arg1	period					1169:1174	flowering period	1159:1174	flowering period	1159:1174	METHODS Lavender essential oils were distilled from lavender plant cultivated in Hungary and the flowers were harvested at the beginning and at the end of flowering period.
34819075	14	115	theme	lavender	2034:2041	arg1	oil/beginning					2053:2065	lavender essential oil/beginning	2034:2065	lavender essential oil/beginning of flowering period	2034:2085	Pretreatments with linalool and lavender essential oil/beginning of flowering period prevented pro-inflammatory cytokine production compared to LPS treatment alone.
34819075	8	116	theme	essential	1021:1029	arg1	oils					1031:1034	essential oils	1021:1034	essential oils	1021:1034	METHODS Lavender essential oils were distilled from lavender plant cultivated in Hungary and the flowers were harvested at the beginning and at the end of flowering period.
34819075	9	117	dep	aeruginosa	1377:1386	arg1	LPS					1388:1390	LPS	1388:1390	P. aeruginosa LPS stimulated inflammation	1374:1414	The experiments were carried out on THP-1 human monocyte/macrophage cell line as in vitro cell culture model for monitoring the effects of lavender essential oils and the main compound linalool on P. aeruginosa LPS stimulated inflammation.
34819075	10	118	theme	Time	1532:1535	arg1	PCR					1537:1539	Real Time PCR	1527:1539	Real Time PCR	1527:1539	The mRNA and protein levels of four pro-inflammatory cytokines, IL-6, IL-1β, IL-8 and TNFα were determined by Real Time PCR and ELISA measurements.
34819075	11	119	theme	NFκB	1632:1635	arg1	luteolin					1649:1656	luteolin	1649:1656	luteolin	1649:1656	The effects of essential oils were compared to the response to two NFκB inhibitors, luteolin and ACHP.
34819075	11	119	theme	NFκB	1632:1635	arg1	inhibitors					1637:1646	two NFκB inhibitors	1628:1646	two NFκB inhibitors	1628:1646	The effects of essential oils were compared to the response to two NFκB inhibitors, luteolin and ACHP.
34819075	11	119	theme	NFκB	1632:1635	arg1	ACHP					1662:1665	ACHP	1662:1665	ACHP	1662:1665	The effects of essential oils were compared to the response to two NFκB inhibitors, luteolin and ACHP.
34819075	15	120	theme	flowering	2206:2214	arg1	period					2216:2221	flowering period	2206:2221	flowering period	2206:2221	Although lavender essential oil/end of flowering period decreased IL-6, IL-1β and IL-8 mRNA expression in case of LPS pretreatment, it was not capable to reduce cytokine secretion.
34819075	14	121	with	Pretreatments	2002:2014	arg1	linalool					2021:2028	linalool	2021:2028	linalool	2021:2028	Pretreatments with linalool and lavender essential oil/beginning of flowering period prevented pro-inflammatory cytokine production compared to LPS treatment alone.
34819075	14	121	with	Pretreatments	2002:2014	arg1	oil/beginning					2053:2065	lavender essential oil/beginning	2034:2065	lavender essential oil/beginning of flowering period	2034:2085	Pretreatments with linalool and lavender essential oil/beginning of flowering period prevented pro-inflammatory cytokine production compared to LPS treatment alone.
34819075	12	122	theme	cytokine	1818:1825	arg1	production					1827:1836	pro-inflammatory cytokine production	1801:1836	pro-inflammatory cytokine production	1801:1836	RESULTS Linalool and lavender essential oil extracted from plants at the beginning of flowering period were successful in decreasing pro-inflammatory cytokine production following LPS pretreatment.
34819075	12	123	dep	RESULTS	1668:1674	arg1	Linalool					1676:1683	Linalool	1676:1683	Linalool	1676:1683	RESULTS Linalool and lavender essential oil extracted from plants at the beginning of flowering period were successful in decreasing pro-inflammatory cytokine production following LPS pretreatment.
34819075	12	123	dep	RESULTS	1668:1674	arg1	oil					1708:1710	lavender essential oil	1689:1710	lavender essential oil	1689:1710	RESULTS Linalool and lavender essential oil extracted from plants at the beginning of flowering period were successful in decreasing pro-inflammatory cytokine production following LPS pretreatment.
34819075	12	123	dep	RESULTS	1668:1674	arg1	RESULTS					1668:1674	RESULTS Linalool and lavender essential oil	1668:1710	RESULTS Linalool and lavender essential oil extracted from plants at the beginning of flowering period	1668:1769	RESULTS Linalool and lavender essential oil extracted from plants at the beginning of flowering period were successful in decreasing pro-inflammatory cytokine production following LPS pretreatment.
34819075	5	124	theme	oils	505:508	arg1	activity					483:490	The anti-inflammatory activity	461:490	The anti-inflammatory activity of essential oils	461:508	The anti-inflammatory activity of essential oils may depend on the composition and the ratio of the compounds.
34819075	16	125	theme	THP-1	2577:2581	arg1	cells					2583:2587	THP-1 cells	2577:2587	THP-1 cells	2577:2587	CONCLUSION Based on our results it has been proven that lavender essential oil extracted at the beginning of flowering period is a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells.
34819075	16	126	theme	synthesis	2503:2511	arg1	oil					2423:2425	lavender essential oil	2404:2425	lavender essential oil extracted at the beginning of flowering period	2404:2472	CONCLUSION Based on our results it has been proven that lavender essential oil extracted at the beginning of flowering period is a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells.
34819075	16	126	theme	synthesis	2503:2511	arg1	inhibitor					2486:2494	a potent inhibitor	2477:2494	a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells	2477:2587	CONCLUSION Based on our results it has been proven that lavender essential oil extracted at the beginning of flowering period is a potent inhibitor of the synthesis of four pro-inflammatory cytokines IL-6, IL-8, IL-β and TNFα of THP-1 cells.
34819075	9	127	theme	THP-1	1213:1217	arg1	line					1250:1253	THP-1 human monocyte/macrophage cell line	1213:1253	THP-1 human monocyte/macrophage cell line	1213:1253	The experiments were carried out on THP-1 human monocyte/macrophage cell line as in vitro cell culture model for monitoring the effects of lavender essential oils and the main compound linalool on P. aeruginosa LPS stimulated inflammation.
34819075	15	128	theme	lavender	2176:2183	arg1	oil/end					2195:2201	lavender essential oil/end	2176:2201	lavender essential oil/end of flowering period	2176:2221	Although lavender essential oil/end of flowering period decreased IL-6, IL-1β and IL-8 mRNA expression in case of LPS pretreatment, it was not capable to reduce cytokine secretion.
34819075	12	129	theme	LPS	1848:1850	arg1	pretreatment					1852:1863	LPS pretreatment	1848:1863	LPS pretreatment	1848:1863	RESULTS Linalool and lavender essential oil extracted from plants at the beginning of flowering period were successful in decreasing pro-inflammatory cytokine production following LPS pretreatment.
34819075	6	130	theme	oils	606:609	arg1	constitution					576:587	The constitution	572:587	The constitution of the essential oils extracted from the different stages of flowering period	572:665	The constitution of the essential oils extracted from the different stages of flowering period varies, which makes it plausible that the collection time of the flowers influences the anti-inflammatory effects.
34819075	9	131	theme	monocyte/macrophage	1225:1243	arg1	line					1250:1253	THP-1 human monocyte/macrophage cell line	1213:1253	THP-1 human monocyte/macrophage cell line	1213:1253	The experiments were carried out on THP-1 human monocyte/macrophage cell line as in vitro cell culture model for monitoring the effects of lavender essential oils and the main compound linalool on P. aeruginosa LPS stimulated inflammation.
34819075	5	132	theme	anti-inflammatory	465:481	arg1	activity					483:490	The anti-inflammatory activity	461:490	The anti-inflammatory activity of essential oils	461:508	The anti-inflammatory activity of essential oils may depend on the composition and the ratio of the compounds.
34819075	7	133	theme	oils	811:814	arg1	types					792:796	Different types	782:796	Different types of essential oils	782:814	Different types of essential oils reduce inflammation acting similarly by modulating the activity and action of the NFκB signalling pathway, which is the major regulator of the transcription of pro-inflammatory cytokines.
33541370	9	0	theme	ultrastructure	1549:1562	arg1	deficits					1628:1635	synaptic ultrastructure, neuroinflammation and brain-derived neurotrophic factor (BDNF) deficits	1540:1635	synaptic ultrastructure, neuroinflammation and brain-derived neurotrophic factor (BDNF) deficits induced by HF diet	1540:1654	In the prefrontal cortex and hippocampus, the β-glucan supplementation ameliorated the alteration of synaptic ultrastructure, neuroinflammation and brain-derived neurotrophic factor (BDNF) deficits induced by HF diet.
33541370	7	1	theme	short-term	1103:1112	arg1	supplementation					1147:1161	short-term and long-term L. edodes β-glucan supplementation	1103:1161	short-term and long-term L. edodes β-glucan supplementation	1103:1161	RESULTS We reported that short-term and long-term L. edodes β-glucan supplementation prevented the gut microbial composition shift induced by the HF diet.
33541370	2	2	theme	edible	284:289	arg1	polysaccharide					291:304	an edible polysaccharide	281:304	an edible polysaccharide isolated from medical mushroom, Lentinula edodes (L. edodes)	281:365	(1, 3)/(1, 6)-β-glucan, an edible polysaccharide isolated from medical mushroom, Lentinula edodes (L. edodes), has the potential to remodel gut microbiota.
33541370	2	2	theme	edible	284:289	arg1	-β-glucan					270:278	(1, 3)/(1, 6)-β-glucan	257:278	-β-glucan	270:278	(1, 3)/(1, 6)-β-glucan, an edible polysaccharide isolated from medical mushroom, Lentinula edodes (L. edodes), has the potential to remodel gut microbiota.
33541370	9	3	theme	neuroinflammation	1565:1581	arg1	deficits					1628:1635	synaptic ultrastructure, neuroinflammation and brain-derived neurotrophic factor (BDNF) deficits	1540:1635	synaptic ultrastructure, neuroinflammation and brain-derived neurotrophic factor (BDNF) deficits induced by HF diet	1540:1654	In the prefrontal cortex and hippocampus, the β-glucan supplementation ameliorated the alteration of synaptic ultrastructure, neuroinflammation and brain-derived neurotrophic factor (BDNF) deficits induced by HF diet.
33541370	12	4	theme	β-glucan	2128:2135	arg1	supplementation					2137:2151	dietary L. edodes β-glucan supplementation	2110:2151	dietary L. edodes β-glucan supplementation	2110:2151	The finding suggested that dietary L. edodes β-glucan supplementation may be an effective nutritional strategy to prevent obesity-associated cognitive decline.
33541370	12	4	theme	β-glucan	2128:2135	arg1	strategy					2185:2192	an effective nutritional strategy	2160:2192	an effective nutritional strategy to prevent obesity-associated cognitive decline	2160:2240	The finding suggested that dietary L. edodes β-glucan supplementation may be an effective nutritional strategy to prevent obesity-associated cognitive decline.
33541370	2	5	contain	has	368:370	arg1	polysaccharide					291:304	an edible polysaccharide	281:304	an edible polysaccharide isolated from medical mushroom, Lentinula edodes (L. edodes)	281:365	(1, 3)/(1, 6)-β-glucan, an edible polysaccharide isolated from medical mushroom, Lentinula edodes (L. edodes), has the potential to remodel gut microbiota.
33541370	2	5	contain	has	368:370	arg1	-β-glucan					270:278	(1, 3)/(1, 6)-β-glucan	257:278	-β-glucan	270:278	(1, 3)/(1, 6)-β-glucan, an edible polysaccharide isolated from medical mushroom, Lentinula edodes (L. edodes), has the potential to remodel gut microbiota.
33541370	2	5	contain	has	368:370	arg2	potential					376:384	the potential to remodel gut microbiota	372:410	the potential to remodel gut microbiota	372:410	(1, 3)/(1, 6)-β-glucan, an edible polysaccharide isolated from medical mushroom, Lentinula edodes (L. edodes), has the potential to remodel gut microbiota.
33541370	11	6	dep	CONCLUSIONS	1922:1932	arg1	revealed					1945:1952	revealed	1945:1952	revealed that L. edodes β-glucan prevents cognitive impairments induced by the HF diet, which may occur via colon-brain axis improvement	1945:2080	CONCLUSIONS This study revealed that L. edodes β-glucan prevents cognitive impairments induced by the HF diet, which may occur via colon-brain axis improvement.
33541370	5	7	dep	METHODS	734:740	arg1	fed					766:768	fed	766:768	were fed with either a lab chow (LC), HF or HF with L. edodes β-glucan supplementation diets for 7 days (short-term) or 15 weeks (long-term)	761:900	METHODS C57BL/6J male mice were fed with either a lab chow (LC), HF or HF with L. edodes β-glucan supplementation diets for 7 days (short-term) or 15 weeks (long-term).
33541370	6	8	dep	endotoxin	1023:1031	arg1	pathology					1067:1075	pathology	1067:1075	pathology	1067:1075	Cognitive behavior was examined; blood, cecum content, colon and brain were collected to evaluate metabolic parameters, endotoxin, gut microbiota, colon, and brain pathology.
33541370	10	9	theme	macrophage	1861:1870	arg1	accumulation					1872:1883	the proinflammatory macrophage accumulation	1841:1883	the proinflammatory macrophage accumulation in the colon of mice fed by HF diet	1841:1919	Furthermore, the β-glucan supplementation increased the mucosal thickness, upregulated the expression of tight junction protein occludin, decreased the plasma LPS level, and inhibited the proinflammatory macrophage accumulation in the colon of mice fed by HF diet.
33541370	3	10	theme	derived	447:453	arg1	β-glucan					455:462	L. edodes derived β-glucan	437:462	L. edodes derived β-glucan	437:462	However, the effects of L. edodes derived β-glucan against HF diet-induced neuroinflammation and cognitive decline remain unknown.
33541370	1	11	theme	diet	167:170	arg1	intake					172:177	BACKGROUND Long-term high fat (HF) diet intake	132:177	BACKGROUND Long-term high fat (HF) diet intake	132:177	BACKGROUND Long-term high fat (HF) diet intake can cause neuroinflammation and cognitive decline through the gut-brain axis.
33541370	9	12	theme	factor	1614:1619	arg1	deficits					1628:1635	synaptic ultrastructure, neuroinflammation and brain-derived neurotrophic factor (BDNF) deficits	1540:1635	synaptic ultrastructure, neuroinflammation and brain-derived neurotrophic factor (BDNF) deficits induced by HF diet	1540:1654	In the prefrontal cortex and hippocampus, the β-glucan supplementation ameliorated the alteration of synaptic ultrastructure, neuroinflammation and brain-derived neurotrophic factor (BDNF) deficits induced by HF diet.
33541370	6	13	theme	gut	1034:1036	arg1	microbiota					1038:1047	gut microbiota	1034:1047	gut microbiota	1034:1047	Cognitive behavior was examined; blood, cecum content, colon and brain were collected to evaluate metabolic parameters, endotoxin, gut microbiota, colon, and brain pathology.
33541370	6	13	theme	gut	1034:1036	arg1	parameters					1011:1020	metabolic parameters	1001:1020	metabolic parameters	1001:1020	Cognitive behavior was examined; blood, cecum content, colon and brain were collected to evaluate metabolic parameters, endotoxin, gut microbiota, colon, and brain pathology.
33541370	11	14	theme	HF	2024:2025	arg1	diet					2027:2030	the HF diet	2020:2030	the HF diet	2020:2030	CONCLUSIONS This study revealed that L. edodes β-glucan prevents cognitive impairments induced by the HF diet, which may occur via colon-brain axis improvement.
33541370	10	15	theme	mice	1901:1904	arg1	colon					1892:1896	the colon	1888:1896	the colon of mice fed by HF diet	1888:1919	Furthermore, the β-glucan supplementation increased the mucosal thickness, upregulated the expression of tight junction protein occludin, decreased the plasma LPS level, and inhibited the proinflammatory macrophage accumulation in the colon of mice fed by HF diet.
33541370	11	16	theme	edodes	1962:1967	arg1	β-glucan					1969:1976	L. edodes β-glucan	1959:1976	L. edodes β-glucan	1959:1976	CONCLUSIONS This study revealed that L. edodes β-glucan prevents cognitive impairments induced by the HF diet, which may occur via colon-brain axis improvement.
33541370	11	17	theme	cognitive	1987:1995	arg1	impairments					1997:2007	cognitive impairments	1987:2007	cognitive impairments	1987:2007	CONCLUSIONS This study revealed that L. edodes β-glucan prevents cognitive impairments induced by the HF diet, which may occur via colon-brain axis improvement.
33541370	8	18	theme	L.	1243:1244	arg1	supplementation					1262:1276	Long-term L. edodes β-glucan supplementation	1233:1276	Long-term L. edodes β-glucan supplementation	1233:1276	Long-term L. edodes β-glucan supplementation prevented the HF diet-induced recognition memory impairment assessed by behavioral tests (the temporal order memory, novel object recognition and Y-maze tests).
33541370	0	19	theme	axis	126:129	arg1	involvement					99:109	involvement	99:109	involvement of colon-brain axis	99:129	β-Glucan from Lentinula edodes prevents cognitive impairments in high-fat diet-induced obese mice: involvement of colon-brain axis.
33541370	3	20	theme	diet-induced	475:486	arg1	neuroinflammation					488:504	HF diet-induced neuroinflammation	472:504	HF diet-induced neuroinflammation	472:504	However, the effects of L. edodes derived β-glucan against HF diet-induced neuroinflammation and cognitive decline remain unknown.
33541370	5	21	theme	L.	813:814	arg1	edodes					816:821	L. edodes	813:821	L. edodes	813:821	METHODS C57BL/6J male mice were fed with either a lab chow (LC), HF or HF with L. edodes β-glucan supplementation diets for 7 days (short-term) or 15 weeks (long-term).
33541370	9	22	theme	deficits	1628:1635	arg1	alteration					1526:1535	the alteration	1522:1535	the alteration of synaptic ultrastructure, neuroinflammation and brain-derived neurotrophic factor (BDNF) deficits induced by HF diet	1522:1654	In the prefrontal cortex and hippocampus, the β-glucan supplementation ameliorated the alteration of synaptic ultrastructure, neuroinflammation and brain-derived neurotrophic factor (BDNF) deficits induced by HF diet.
33541370	5	23	dep	15 weeks	881:888	arg1	long-term					891:899	long-term	891:899	long-term	891:899	METHODS C57BL/6J male mice were fed with either a lab chow (LC), HF or HF with L. edodes β-glucan supplementation diets for 7 days (short-term) or 15 weeks (long-term).
33541370	1	24	theme	cognitive	211:219	arg1	decline					221:227	cognitive decline	211:227	cognitive decline	211:227	BACKGROUND Long-term high fat (HF) diet intake can cause neuroinflammation and cognitive decline through the gut-brain axis.
33541370	3	25	dep	derived	447:453	arg1	L.					437:438	L.	437:438	L.	437:438	However, the effects of L. edodes derived β-glucan against HF diet-induced neuroinflammation and cognitive decline remain unknown.
33541370	4	26	theme	dietary	617:623	arg1	supplementation					643:657	dietary L edodes β-glucan supplementation	617:657	dietary L edodes β-glucan supplementation	617:657	This study aimed to evaluate the neuroprotective effect and mechanism of dietary L edodes β-glucan supplementation against the obesity-associated cognitive decline in mice fed by a HF diet.
33541370	4	27	theme	obesity-associated	671:688	arg1	decline					700:706	the obesity-associated cognitive decline	667:706	the obesity-associated cognitive decline in mice fed by a HF diet	667:731	This study aimed to evaluate the neuroprotective effect and mechanism of dietary L edodes β-glucan supplementation against the obesity-associated cognitive decline in mice fed by a HF diet.
33541370	10	28	theme	tight	1762:1766	arg1	occludin					1785:1792	tight junction protein occludin	1762:1792	tight junction protein occludin	1762:1792	Furthermore, the β-glucan supplementation increased the mucosal thickness, upregulated the expression of tight junction protein occludin, decreased the plasma LPS level, and inhibited the proinflammatory macrophage accumulation in the colon of mice fed by HF diet.
33541370	5	29	theme	β-glucan	823:830	arg1	diets					848:852	β-glucan supplementation diets	823:852	β-glucan supplementation diets	823:852	METHODS C57BL/6J male mice were fed with either a lab chow (LC), HF or HF with L. edodes β-glucan supplementation diets for 7 days (short-term) or 15 weeks (long-term).
33541370	8	30	theme	order	1381:1385	arg1	tests					1361:1365	behavioral tests	1350:1365	behavioral tests (the temporal order memory, novel object recognition and Y-maze tests)	1350:1436	Long-term L. edodes β-glucan supplementation prevented the HF diet-induced recognition memory impairment assessed by behavioral tests (the temporal order memory, novel object recognition and Y-maze tests).
33541370	8	30	theme	order	1381:1385	arg1	memory					1387:1392	the temporal order memory	1368:1392	the temporal order memory	1368:1392	Long-term L. edodes β-glucan supplementation prevented the HF diet-induced recognition memory impairment assessed by behavioral tests (the temporal order memory, novel object recognition and Y-maze tests).
33541370	7	31	theme	HF	1224:1225	arg1	diet					1227:1230	the HF diet	1220:1230	the HF diet	1220:1230	RESULTS We reported that short-term and long-term L. edodes β-glucan supplementation prevented the gut microbial composition shift induced by the HF diet.
33541370	4	32	theme	L	625:625	arg1	supplementation					643:657	dietary L edodes β-glucan supplementation	617:657	dietary L edodes β-glucan supplementation	617:657	This study aimed to evaluate the neuroprotective effect and mechanism of dietary L edodes β-glucan supplementation against the obesity-associated cognitive decline in mice fed by a HF diet.
33541370	0	33	theme	high-fat	65:72	arg1	mice					93:96	high-fat diet-induced obese mice	65:96	high-fat diet-induced obese mice	65:96	β-Glucan from Lentinula edodes prevents cognitive impairments in high-fat diet-induced obese mice: involvement of colon-brain axis.
33541370	1	34	theme	fat	158:160	arg1	intake					172:177	BACKGROUND Long-term high fat (HF) diet intake	132:177	BACKGROUND Long-term high fat (HF) diet intake	132:177	BACKGROUND Long-term high fat (HF) diet intake can cause neuroinflammation and cognitive decline through the gut-brain axis.
33541370	12	35	theme	cognitive	2224:2232	arg1	decline					2234:2240	obesity-associated cognitive decline	2205:2240	obesity-associated cognitive decline	2205:2240	The finding suggested that dietary L. edodes β-glucan supplementation may be an effective nutritional strategy to prevent obesity-associated cognitive decline.
33541370	4	36	theme	β-glucan	634:641	arg1	supplementation					643:657	dietary L edodes β-glucan supplementation	617:657	dietary L edodes β-glucan supplementation	617:657	This study aimed to evaluate the neuroprotective effect and mechanism of dietary L edodes β-glucan supplementation against the obesity-associated cognitive decline in mice fed by a HF diet.
33541370	0	37	theme	obese	87:91	arg1	mice					93:96	high-fat diet-induced obese mice	65:96	high-fat diet-induced obese mice	65:96	β-Glucan from Lentinula edodes prevents cognitive impairments in high-fat diet-induced obese mice: involvement of colon-brain axis.
33541370	8	38	theme	object	1401:1406	arg1	tests					1361:1365	behavioral tests	1350:1365	behavioral tests (the temporal order memory, novel object recognition and Y-maze tests)	1350:1436	Long-term L. edodes β-glucan supplementation prevented the HF diet-induced recognition memory impairment assessed by behavioral tests (the temporal order memory, novel object recognition and Y-maze tests).
33541370	8	38	theme	object	1401:1406	arg1	recognition					1408:1418	novel object recognition	1395:1418	novel object recognition	1395:1418	Long-term L. edodes β-glucan supplementation prevented the HF diet-induced recognition memory impairment assessed by behavioral tests (the temporal order memory, novel object recognition and Y-maze tests).
33541370	12	39	theme	edodes	2121:2126	arg1	supplementation					2137:2151	dietary L. edodes β-glucan supplementation	2110:2151	dietary L. edodes β-glucan supplementation	2110:2151	The finding suggested that dietary L. edodes β-glucan supplementation may be an effective nutritional strategy to prevent obesity-associated cognitive decline.
33541370	12	39	theme	edodes	2121:2126	arg1	strategy					2185:2192	an effective nutritional strategy	2160:2192	an effective nutritional strategy to prevent obesity-associated cognitive decline	2160:2240	The finding suggested that dietary L. edodes β-glucan supplementation may be an effective nutritional strategy to prevent obesity-associated cognitive decline.
33541370	1	40	theme	Long-term	143:151	arg1	intake					172:177	BACKGROUND Long-term high fat (HF) diet intake	132:177	BACKGROUND Long-term high fat (HF) diet intake	132:177	BACKGROUND Long-term high fat (HF) diet intake can cause neuroinflammation and cognitive decline through the gut-brain axis.
33541370	0	41	from	impairments	50:60	arg1	mice					93:96	high-fat diet-induced obese mice	65:96	high-fat diet-induced obese mice	65:96	β-Glucan from Lentinula edodes prevents cognitive impairments in high-fat diet-induced obese mice: involvement of colon-brain axis.
33541370	12	42	theme	L.	2118:2119	arg1	supplementation					2137:2151	dietary L. edodes β-glucan supplementation	2110:2151	dietary L. edodes β-glucan supplementation	2110:2151	The finding suggested that dietary L. edodes β-glucan supplementation may be an effective nutritional strategy to prevent obesity-associated cognitive decline.
33541370	12	42	theme	L.	2118:2119	arg1	strategy					2185:2192	an effective nutritional strategy	2160:2192	an effective nutritional strategy to prevent obesity-associated cognitive decline	2160:2240	The finding suggested that dietary L. edodes β-glucan supplementation may be an effective nutritional strategy to prevent obesity-associated cognitive decline.
33541370	4	43	theme	HF	725:726	arg1	diet					728:731	a HF diet	723:731	a HF diet	723:731	This study aimed to evaluate the neuroprotective effect and mechanism of dietary L edodes β-glucan supplementation against the obesity-associated cognitive decline in mice fed by a HF diet.
33541370	9	44	theme	prefrontal	1446:1455	arg1	cortex					1457:1462	the prefrontal cortex	1442:1462	the prefrontal cortex	1442:1462	In the prefrontal cortex and hippocampus, the β-glucan supplementation ameliorated the alteration of synaptic ultrastructure, neuroinflammation and brain-derived neurotrophic factor (BDNF) deficits induced by HF diet.
33541370	8	45	theme	edodes	1246:1251	arg1	supplementation					1262:1276	Long-term L. edodes β-glucan supplementation	1233:1276	Long-term L. edodes β-glucan supplementation	1233:1276	Long-term L. edodes β-glucan supplementation prevented the HF diet-induced recognition memory impairment assessed by behavioral tests (the temporal order memory, novel object recognition and Y-maze tests).
33541370	4	46	from	decline	700:706	arg1	mice					711:714	mice	711:714	mice fed by a HF diet	711:731	This study aimed to evaluate the neuroprotective effect and mechanism of dietary L edodes β-glucan supplementation against the obesity-associated cognitive decline in mice fed by a HF diet.
33541370	9	47	theme	brain-derived	1587:1599	arg1	BDNF					1622:1625	BDNF	1622:1625	BDNF	1622:1625	In the prefrontal cortex and hippocampus, the β-glucan supplementation ameliorated the alteration of synaptic ultrastructure, neuroinflammation and brain-derived neurotrophic factor (BDNF) deficits induced by HF diet.
33541370	9	47	theme	brain-derived	1587:1599	arg1	factor					1614:1619	brain-derived neurotrophic factor	1587:1619	brain-derived neurotrophic factor (BDNF)	1587:1626	In the prefrontal cortex and hippocampus, the β-glucan supplementation ameliorated the alteration of synaptic ultrastructure, neuroinflammation and brain-derived neurotrophic factor (BDNF) deficits induced by HF diet.
33541370	6	48	theme	cecum	943:947	arg1	content					949:955	cecum content	943:955	cecum content	943:955	Cognitive behavior was examined; blood, cecum content, colon and brain were collected to evaluate metabolic parameters, endotoxin, gut microbiota, colon, and brain pathology.
33541370	7	49	dep	RESULTS	1078:1084	arg1	reported					1089:1096	reported	1089:1096	reported that short-term and long-term L. edodes β-glucan supplementation prevented the gut microbial composition shift induced by the HF diet	1089:1230	RESULTS We reported that short-term and long-term L. edodes β-glucan supplementation prevented the gut microbial composition shift induced by the HF diet.
33541370	0	50	theme	Lentinula	14:22	arg1	edodes					24:29	Lentinula edodes	14:29	Lentinula edodes	14:29	β-Glucan from Lentinula edodes prevents cognitive impairments in high-fat diet-induced obese mice: involvement of colon-brain axis.
33541370	3	51	dep	L.	437:438	arg1	edodes					440:445	L. edodes	437:445	L. edodes	437:445	However, the effects of L. edodes derived β-glucan against HF diet-induced neuroinflammation and cognitive decline remain unknown.
33541370	7	52	theme	composition	1191:1201	arg1	shift					1203:1207	the gut microbial composition shift	1173:1207	the gut microbial composition shift induced by the HF diet	1173:1230	RESULTS We reported that short-term and long-term L. edodes β-glucan supplementation prevented the gut microbial composition shift induced by the HF diet.
33541370	2	53	theme	gut	397:399	arg1	microbiota					401:410	gut microbiota	397:410	gut microbiota	397:410	(1, 3)/(1, 6)-β-glucan, an edible polysaccharide isolated from medical mushroom, Lentinula edodes (L. edodes), has the potential to remodel gut microbiota.
33541370	12	54	theme	nutritional	2173:2183	arg1	supplementation					2137:2151	dietary L. edodes β-glucan supplementation	2110:2151	dietary L. edodes β-glucan supplementation	2110:2151	The finding suggested that dietary L. edodes β-glucan supplementation may be an effective nutritional strategy to prevent obesity-associated cognitive decline.
33541370	12	54	theme	nutritional	2173:2183	arg1	strategy					2185:2192	an effective nutritional strategy	2160:2192	an effective nutritional strategy to prevent obesity-associated cognitive decline	2160:2240	The finding suggested that dietary L. edodes β-glucan supplementation may be an effective nutritional strategy to prevent obesity-associated cognitive decline.
33541370	9	55	theme	β-glucan	1485:1492	arg1	supplementation					1494:1508	the β-glucan supplementation	1481:1508	the β-glucan supplementation	1481:1508	In the prefrontal cortex and hippocampus, the β-glucan supplementation ameliorated the alteration of synaptic ultrastructure, neuroinflammation and brain-derived neurotrophic factor (BDNF) deficits induced by HF diet.
33541370	7	56	theme	gut	1177:1179	arg1	shift					1203:1207	the gut microbial composition shift	1173:1207	the gut microbial composition shift induced by the HF diet	1173:1230	RESULTS We reported that short-term and long-term L. edodes β-glucan supplementation prevented the gut microbial composition shift induced by the HF diet.
33541370	10	57	theme	protein	1777:1783	arg1	occludin					1785:1792	tight junction protein occludin	1762:1792	tight junction protein occludin	1762:1792	Furthermore, the β-glucan supplementation increased the mucosal thickness, upregulated the expression of tight junction protein occludin, decreased the plasma LPS level, and inhibited the proinflammatory macrophage accumulation in the colon of mice fed by HF diet.
33541370	5	58	dep	chow	788:791	arg1	diets					848:852	β-glucan supplementation diets	823:852	β-glucan supplementation diets	823:852	METHODS C57BL/6J male mice were fed with either a lab chow (LC), HF or HF with L. edodes β-glucan supplementation diets for 7 days (short-term) or 15 weeks (long-term).
33541370	5	59	theme	C57BL/6J	742:749	arg1	mice					756:759	C57BL/6J male mice	742:759	C57BL/6J male mice	742:759	METHODS C57BL/6J male mice were fed with either a lab chow (LC), HF or HF with L. edodes β-glucan supplementation diets for 7 days (short-term) or 15 weeks (long-term).
33541370	0	60	from	edodes	24:29	arg1	β-Glucan					0:7	β-Glucan	0:7	β-Glucan from Lentinula edodes	0:29	β-Glucan from Lentinula edodes prevents cognitive impairments in high-fat diet-induced obese mice: involvement of colon-brain axis.
33541370	0	61	dep	prevents	31:38	arg1	involvement					99:109	involvement	99:109	involvement of colon-brain axis	99:129	β-Glucan from Lentinula edodes prevents cognitive impairments in high-fat diet-induced obese mice: involvement of colon-brain axis.
33541370	6	62	theme	metabolic	1001:1009	arg1	brain					1061:1065	brain	1061:1065	brain	1061:1065	Cognitive behavior was examined; blood, cecum content, colon and brain were collected to evaluate metabolic parameters, endotoxin, gut microbiota, colon, and brain pathology.
33541370	6	62	theme	metabolic	1001:1009	arg1	colon					1050:1054	colon	1050:1054	colon	1050:1054	Cognitive behavior was examined; blood, cecum content, colon and brain were collected to evaluate metabolic parameters, endotoxin, gut microbiota, colon, and brain pathology.
33541370	6	62	theme	metabolic	1001:1009	arg1	endotoxin					1023:1031	endotoxin	1023:1031	endotoxin	1023:1031	Cognitive behavior was examined; blood, cecum content, colon and brain were collected to evaluate metabolic parameters, endotoxin, gut microbiota, colon, and brain pathology.
33541370	6	62	theme	metabolic	1001:1009	arg1	microbiota					1038:1047	gut microbiota	1034:1047	gut microbiota	1034:1047	Cognitive behavior was examined; blood, cecum content, colon and brain were collected to evaluate metabolic parameters, endotoxin, gut microbiota, colon, and brain pathology.
33541370	6	62	theme	metabolic	1001:1009	arg1	parameters					1011:1020	metabolic parameters	1001:1020	metabolic parameters	1001:1020	Cognitive behavior was examined; blood, cecum content, colon and brain were collected to evaluate metabolic parameters, endotoxin, gut microbiota, colon, and brain pathology.
33541370	5	63	with	chow	788:791	arg1	edodes					816:821	L. edodes	813:821	L. edodes	813:821	METHODS C57BL/6J male mice were fed with either a lab chow (LC), HF or HF with L. edodes β-glucan supplementation diets for 7 days (short-term) or 15 weeks (long-term).
33541370	10	64	theme	LPS	1816:1818	arg1	level					1820:1824	the plasma LPS level	1805:1824	the plasma LPS level	1805:1824	Furthermore, the β-glucan supplementation increased the mucosal thickness, upregulated the expression of tight junction protein occludin, decreased the plasma LPS level, and inhibited the proinflammatory macrophage accumulation in the colon of mice fed by HF diet.
33541370	8	65	theme	memory	1320:1325	arg1	impairment					1327:1336	the HF diet-induced recognition memory impairment	1288:1336	the HF diet-induced recognition memory impairment assessed by behavioral tests (the temporal order memory, novel object recognition and Y-maze tests)	1288:1436	Long-term L. edodes β-glucan supplementation prevented the HF diet-induced recognition memory impairment assessed by behavioral tests (the temporal order memory, novel object recognition and Y-maze tests).
33541370	7	66	theme	β-glucan	1138:1145	arg1	supplementation					1147:1161	short-term and long-term L. edodes β-glucan supplementation	1103:1161	short-term and long-term L. edodes β-glucan supplementation	1103:1161	RESULTS We reported that short-term and long-term L. edodes β-glucan supplementation prevented the gut microbial composition shift induced by the HF diet.
33541370	2	67	attach	isolated	306:313	arg2	polysaccharide					291:304	an edible polysaccharide	281:304	an edible polysaccharide isolated from medical mushroom, Lentinula edodes (L. edodes)	281:365	(1, 3)/(1, 6)-β-glucan, an edible polysaccharide isolated from medical mushroom, Lentinula edodes (L. edodes), has the potential to remodel gut microbiota.
33541370	2	67	attach	isolated	306:313	arg2	-β-glucan					270:278	(1, 3)/(1, 6)-β-glucan	257:278	-β-glucan	270:278	(1, 3)/(1, 6)-β-glucan, an edible polysaccharide isolated from medical mushroom, Lentinula edodes (L. edodes), has the potential to remodel gut microbiota.
33541370	2	67	attach	isolated	306:313	arg1	edodes					348:353	edodes	348:353	edodes	348:353	(1, 3)/(1, 6)-β-glucan, an edible polysaccharide isolated from medical mushroom, Lentinula edodes (L. edodes), has the potential to remodel gut microbiota.
33541370	2	67	attach	isolated	306:313	arg1	edodes					359:364	edodes	359:364	edodes	359:364	(1, 3)/(1, 6)-β-glucan, an edible polysaccharide isolated from medical mushroom, Lentinula edodes (L. edodes), has the potential to remodel gut microbiota.
33541370	9	68	theme	synaptic	1540:1547	arg1	ultrastructure					1549:1562	synaptic ultrastructure	1540:1562	synaptic ultrastructure	1540:1562	In the prefrontal cortex and hippocampus, the β-glucan supplementation ameliorated the alteration of synaptic ultrastructure, neuroinflammation and brain-derived neurotrophic factor (BDNF) deficits induced by HF diet.
33541370	7	69	theme	L.	1128:1129	arg1	supplementation					1147:1161	short-term and long-term L. edodes β-glucan supplementation	1103:1161	short-term and long-term L. edodes β-glucan supplementation	1103:1161	RESULTS We reported that short-term and long-term L. edodes β-glucan supplementation prevented the gut microbial composition shift induced by the HF diet.
33541370	11	70	theme	colon-brain	2053:2063	arg1	improvement					2070:2080	colon-brain axis improvement	2053:2080	colon-brain axis improvement	2053:2080	CONCLUSIONS This study revealed that L. edodes β-glucan prevents cognitive impairments induced by the HF diet, which may occur via colon-brain axis improvement.
33541370	8	71	theme	diet-induced	1295:1306	arg1	impairment					1327:1336	the HF diet-induced recognition memory impairment	1288:1336	the HF diet-induced recognition memory impairment assessed by behavioral tests (the temporal order memory, novel object recognition and Y-maze tests)	1288:1436	Long-term L. edodes β-glucan supplementation prevented the HF diet-induced recognition memory impairment assessed by behavioral tests (the temporal order memory, novel object recognition and Y-maze tests).
33541370	3	72	theme	β-glucan	455:462	arg1	effects					426:432	the effects	422:432	the effects of L. edodes derived β-glucan against HF diet-induced neuroinflammation and cognitive decline	422:526	However, the effects of L. edodes derived β-glucan against HF diet-induced neuroinflammation and cognitive decline remain unknown.
33541370	11	73	theme	L.	1959:1960	arg1	β-glucan					1969:1976	L. edodes β-glucan	1959:1976	L. edodes β-glucan	1959:1976	CONCLUSIONS This study revealed that L. edodes β-glucan prevents cognitive impairments induced by the HF diet, which may occur via colon-brain axis improvement.
33541370	9	74	theme	neurotrophic	1601:1612	arg1	BDNF					1622:1625	BDNF	1622:1625	BDNF	1622:1625	In the prefrontal cortex and hippocampus, the β-glucan supplementation ameliorated the alteration of synaptic ultrastructure, neuroinflammation and brain-derived neurotrophic factor (BDNF) deficits induced by HF diet.
33541370	9	74	theme	neurotrophic	1601:1612	arg1	factor					1614:1619	brain-derived neurotrophic factor	1587:1619	brain-derived neurotrophic factor (BDNF)	1587:1626	In the prefrontal cortex and hippocampus, the β-glucan supplementation ameliorated the alteration of synaptic ultrastructure, neuroinflammation and brain-derived neurotrophic factor (BDNF) deficits induced by HF diet.
33541370	10	75	theme	β-glucan	1674:1681	arg1	supplementation					1683:1697	the β-glucan supplementation	1670:1697	the β-glucan supplementation	1670:1697	Furthermore, the β-glucan supplementation increased the mucosal thickness, upregulated the expression of tight junction protein occludin, decreased the plasma LPS level, and inhibited the proinflammatory macrophage accumulation in the colon of mice fed by HF diet.
33541370	10	76	theme	proinflammatory	1845:1859	arg1	accumulation					1872:1883	the proinflammatory macrophage accumulation	1841:1883	the proinflammatory macrophage accumulation in the colon of mice fed by HF diet	1841:1919	Furthermore, the β-glucan supplementation increased the mucosal thickness, upregulated the expression of tight junction protein occludin, decreased the plasma LPS level, and inhibited the proinflammatory macrophage accumulation in the colon of mice fed by HF diet.
33541370	10	77	theme	mucosal	1713:1719	arg1	thickness					1721:1729	the mucosal thickness	1709:1729	the mucosal thickness	1709:1729	Furthermore, the β-glucan supplementation increased the mucosal thickness, upregulated the expression of tight junction protein occludin, decreased the plasma LPS level, and inhibited the proinflammatory macrophage accumulation in the colon of mice fed by HF diet.
33541370	12	78	theme	dietary	2110:2116	arg1	supplementation					2137:2151	dietary L. edodes β-glucan supplementation	2110:2151	dietary L. edodes β-glucan supplementation	2110:2151	The finding suggested that dietary L. edodes β-glucan supplementation may be an effective nutritional strategy to prevent obesity-associated cognitive decline.
33541370	12	78	theme	dietary	2110:2116	arg1	strategy					2185:2192	an effective nutritional strategy	2160:2192	an effective nutritional strategy to prevent obesity-associated cognitive decline	2160:2240	The finding suggested that dietary L. edodes β-glucan supplementation may be an effective nutritional strategy to prevent obesity-associated cognitive decline.
33541370	3	79	theme	HF	472:473	arg1	neuroinflammation					488:504	HF diet-induced neuroinflammation	472:504	HF diet-induced neuroinflammation	472:504	However, the effects of L. edodes derived β-glucan against HF diet-induced neuroinflammation and cognitive decline remain unknown.
33541370	8	80	theme	behavioral	1350:1359	arg1	tests					1361:1365	behavioral tests	1350:1365	behavioral tests (the temporal order memory, novel object recognition and Y-maze tests)	1350:1436	Long-term L. edodes β-glucan supplementation prevented the HF diet-induced recognition memory impairment assessed by behavioral tests (the temporal order memory, novel object recognition and Y-maze tests).
33541370	8	80	theme	behavioral	1350:1359	arg1	recognition					1408:1418	novel object recognition	1395:1418	novel object recognition	1395:1418	Long-term L. edodes β-glucan supplementation prevented the HF diet-induced recognition memory impairment assessed by behavioral tests (the temporal order memory, novel object recognition and Y-maze tests).
33541370	8	80	theme	behavioral	1350:1359	arg1	memory					1387:1392	the temporal order memory	1368:1392	the temporal order memory	1368:1392	Long-term L. edodes β-glucan supplementation prevented the HF diet-induced recognition memory impairment assessed by behavioral tests (the temporal order memory, novel object recognition and Y-maze tests).
33541370	10	81	theme	HF	1913:1914	arg1	diet					1916:1919	HF diet	1913:1919	HF diet	1913:1919	Furthermore, the β-glucan supplementation increased the mucosal thickness, upregulated the expression of tight junction protein occludin, decreased the plasma LPS level, and inhibited the proinflammatory macrophage accumulation in the colon of mice fed by HF diet.
33541370	3	82	theme	cognitive	510:518	arg1	decline					520:526	cognitive decline	510:526	cognitive decline	510:526	However, the effects of L. edodes derived β-glucan against HF diet-induced neuroinflammation and cognitive decline remain unknown.
33541370	10	83	from	accumulation	1872:1883	arg1	colon					1892:1896	the colon	1888:1896	the colon of mice fed by HF diet	1888:1919	Furthermore, the β-glucan supplementation increased the mucosal thickness, upregulated the expression of tight junction protein occludin, decreased the plasma LPS level, and inhibited the proinflammatory macrophage accumulation in the colon of mice fed by HF diet.
33541370	9	84	theme	HF	1648:1649	arg1	diet					1651:1654	HF diet	1648:1654	HF diet	1648:1654	In the prefrontal cortex and hippocampus, the β-glucan supplementation ameliorated the alteration of synaptic ultrastructure, neuroinflammation and brain-derived neurotrophic factor (BDNF) deficits induced by HF diet.
33541370	8	85	theme	temporal	1372:1379	arg1	tests					1361:1365	behavioral tests	1350:1365	behavioral tests (the temporal order memory, novel object recognition and Y-maze tests)	1350:1436	Long-term L. edodes β-glucan supplementation prevented the HF diet-induced recognition memory impairment assessed by behavioral tests (the temporal order memory, novel object recognition and Y-maze tests).
33541370	8	85	theme	temporal	1372:1379	arg1	memory					1387:1392	the temporal order memory	1368:1392	the temporal order memory	1368:1392	Long-term L. edodes β-glucan supplementation prevented the HF diet-induced recognition memory impairment assessed by behavioral tests (the temporal order memory, novel object recognition and Y-maze tests).
33541370	4	86	theme	supplementation	643:657	arg1	mechanism					604:612	mechanism	604:612	mechanism	604:612	This study aimed to evaluate the neuroprotective effect and mechanism of dietary L edodes β-glucan supplementation against the obesity-associated cognitive decline in mice fed by a HF diet.
33541370	4	86	theme	supplementation	643:657	arg1	effect					593:598	the neuroprotective effect	573:598	the neuroprotective effect	573:598	This study aimed to evaluate the neuroprotective effect and mechanism of dietary L edodes β-glucan supplementation against the obesity-associated cognitive decline in mice fed by a HF diet.
33541370	10	87	theme	junction	1768:1775	arg1	occludin					1785:1792	tight junction protein occludin	1762:1792	tight junction protein occludin	1762:1792	Furthermore, the β-glucan supplementation increased the mucosal thickness, upregulated the expression of tight junction protein occludin, decreased the plasma LPS level, and inhibited the proinflammatory macrophage accumulation in the colon of mice fed by HF diet.
33541370	1	88	theme	BACKGROUND	132:141	arg1	intake					172:177	BACKGROUND Long-term high fat (HF) diet intake	132:177	BACKGROUND Long-term high fat (HF) diet intake	132:177	BACKGROUND Long-term high fat (HF) diet intake can cause neuroinflammation and cognitive decline through the gut-brain axis.
33541370	0	89	theme	diet-induced	74:85	arg1	mice					93:96	high-fat diet-induced obese mice	65:96	high-fat diet-induced obese mice	65:96	β-Glucan from Lentinula edodes prevents cognitive impairments in high-fat diet-induced obese mice: involvement of colon-brain axis.
33541370	1	90	theme	high	153:156	arg1	fat					158:160	high fat	153:160	BACKGROUND Long-term high fat (HF) diet intake	132:177	BACKGROUND Long-term high fat (HF) diet intake can cause neuroinflammation and cognitive decline through the gut-brain axis.
33541370	1	90	theme	high	153:156	arg1	HF					163:164	HF	163:164	HF	163:164	BACKGROUND Long-term high fat (HF) diet intake can cause neuroinflammation and cognitive decline through the gut-brain axis.
33541370	6	91	theme	Cognitive	903:911	arg1	behavior					913:920	Cognitive behavior	903:920	Cognitive behavior	903:920	Cognitive behavior was examined; blood, cecum content, colon and brain were collected to evaluate metabolic parameters, endotoxin, gut microbiota, colon, and brain pathology.
33541370	8	92	theme	novel	1395:1399	arg1	tests					1361:1365	behavioral tests	1350:1365	behavioral tests (the temporal order memory, novel object recognition and Y-maze tests)	1350:1436	Long-term L. edodes β-glucan supplementation prevented the HF diet-induced recognition memory impairment assessed by behavioral tests (the temporal order memory, novel object recognition and Y-maze tests).
33541370	8	92	theme	novel	1395:1399	arg1	recognition					1408:1418	novel object recognition	1395:1418	novel object recognition	1395:1418	Long-term L. edodes β-glucan supplementation prevented the HF diet-induced recognition memory impairment assessed by behavioral tests (the temporal order memory, novel object recognition and Y-maze tests).
33541370	5	93	dep	7 days	858:863	arg1	short-term					866:875	short-term	866:875	short-term	866:875	METHODS C57BL/6J male mice were fed with either a lab chow (LC), HF or HF with L. edodes β-glucan supplementation diets for 7 days (short-term) or 15 weeks (long-term).
33541370	5	94	with	HF	805:806	arg1	edodes					816:821	L. edodes	813:821	L. edodes	813:821	METHODS C57BL/6J male mice were fed with either a lab chow (LC), HF or HF with L. edodes β-glucan supplementation diets for 7 days (short-term) or 15 weeks (long-term).
33541370	4	95	theme	edodes	627:632	arg1	supplementation					643:657	dietary L edodes β-glucan supplementation	617:657	dietary L edodes β-glucan supplementation	617:657	This study aimed to evaluate the neuroprotective effect and mechanism of dietary L edodes β-glucan supplementation against the obesity-associated cognitive decline in mice fed by a HF diet.
33541370	3	96	link	derived	447:453	arg1	β-glucan					455:462	L. edodes derived β-glucan	437:462	L. edodes derived β-glucan	437:462	However, the effects of L. edodes derived β-glucan against HF diet-induced neuroinflammation and cognitive decline remain unknown.
33541370	8	97	theme	Y-maze	1424:1429	arg1	tests					1431:1435	Y-maze tests	1424:1435	Y-maze tests	1424:1435	Long-term L. edodes β-glucan supplementation prevented the HF diet-induced recognition memory impairment assessed by behavioral tests (the temporal order memory, novel object recognition and Y-maze tests).
33541370	5	98	with	HF	799:800	arg1	edodes					816:821	L. edodes	813:821	L. edodes	813:821	METHODS C57BL/6J male mice were fed with either a lab chow (LC), HF or HF with L. edodes β-glucan supplementation diets for 7 days (short-term) or 15 weeks (long-term).
33541370	0	99	theme	colon-brain	114:124	arg1	axis					126:129	colon-brain axis	114:129	colon-brain axis	114:129	β-Glucan from Lentinula edodes prevents cognitive impairments in high-fat diet-induced obese mice: involvement of colon-brain axis.
33541370	12	100	theme	obesity-associated	2205:2222	arg1	decline					2234:2240	obesity-associated cognitive decline	2205:2240	obesity-associated cognitive decline	2205:2240	The finding suggested that dietary L. edodes β-glucan supplementation may be an effective nutritional strategy to prevent obesity-associated cognitive decline.
33541370	11	101	theme	axis	2065:2068	arg1	improvement					2070:2080	colon-brain axis improvement	2053:2080	colon-brain axis improvement	2053:2080	CONCLUSIONS This study revealed that L. edodes β-glucan prevents cognitive impairments induced by the HF diet, which may occur via colon-brain axis improvement.
33541370	12	102	theme	effective	2163:2171	arg1	supplementation					2137:2151	dietary L. edodes β-glucan supplementation	2110:2151	dietary L. edodes β-glucan supplementation	2110:2151	The finding suggested that dietary L. edodes β-glucan supplementation may be an effective nutritional strategy to prevent obesity-associated cognitive decline.
33541370	12	102	theme	effective	2163:2171	arg1	strategy					2185:2192	an effective nutritional strategy	2160:2192	an effective nutritional strategy to prevent obesity-associated cognitive decline	2160:2240	The finding suggested that dietary L. edodes β-glucan supplementation may be an effective nutritional strategy to prevent obesity-associated cognitive decline.
33541370	5	103	theme	male	751:754	arg1	mice					756:759	C57BL/6J male mice	742:759	C57BL/6J male mice	742:759	METHODS C57BL/6J male mice were fed with either a lab chow (LC), HF or HF with L. edodes β-glucan supplementation diets for 7 days (short-term) or 15 weeks (long-term).
33541370	5	104	theme	supplementation	832:846	arg1	diets					848:852	β-glucan supplementation diets	823:852	β-glucan supplementation diets	823:852	METHODS C57BL/6J male mice were fed with either a lab chow (LC), HF or HF with L. edodes β-glucan supplementation diets for 7 days (short-term) or 15 weeks (long-term).
33541370	9	105	link	brain-derived	1587:1599	arg1	BDNF					1622:1625	BDNF	1622:1625	BDNF	1622:1625	In the prefrontal cortex and hippocampus, the β-glucan supplementation ameliorated the alteration of synaptic ultrastructure, neuroinflammation and brain-derived neurotrophic factor (BDNF) deficits induced by HF diet.
33541370	9	105	link	brain-derived	1587:1599	arg1	factor					1614:1619	brain-derived neurotrophic factor	1587:1619	brain-derived neurotrophic factor (BDNF)	1587:1626	In the prefrontal cortex and hippocampus, the β-glucan supplementation ameliorated the alteration of synaptic ultrastructure, neuroinflammation and brain-derived neurotrophic factor (BDNF) deficits induced by HF diet.
33541370	4	106	theme	cognitive	690:698	arg1	decline					700:706	the obesity-associated cognitive decline	667:706	the obesity-associated cognitive decline in mice fed by a HF diet	667:731	This study aimed to evaluate the neuroprotective effect and mechanism of dietary L edodes β-glucan supplementation against the obesity-associated cognitive decline in mice fed by a HF diet.
33541370	7	107	theme	microbial	1181:1189	arg1	shift					1203:1207	the gut microbial composition shift	1173:1207	the gut microbial composition shift induced by the HF diet	1173:1230	RESULTS We reported that short-term and long-term L. edodes β-glucan supplementation prevented the gut microbial composition shift induced by the HF diet.
33541370	8	108	theme	Long-term	1233:1241	arg1	supplementation					1262:1276	Long-term L. edodes β-glucan supplementation	1233:1276	Long-term L. edodes β-glucan supplementation	1233:1276	Long-term L. edodes β-glucan supplementation prevented the HF diet-induced recognition memory impairment assessed by behavioral tests (the temporal order memory, novel object recognition and Y-maze tests).
33541370	8	109	theme	β-glucan	1253:1260	arg1	supplementation					1262:1276	Long-term L. edodes β-glucan supplementation	1233:1276	Long-term L. edodes β-glucan supplementation	1233:1276	Long-term L. edodes β-glucan supplementation prevented the HF diet-induced recognition memory impairment assessed by behavioral tests (the temporal order memory, novel object recognition and Y-maze tests).
33541370	0	110	theme	cognitive	40:48	arg1	impairments					50:60	cognitive impairments	40:60	cognitive impairments in high-fat diet-induced obese mice	40:96	β-Glucan from Lentinula edodes prevents cognitive impairments in high-fat diet-induced obese mice: involvement of colon-brain axis.
33541370	4	111	theme	neuroprotective	577:591	arg1	effect					593:598	the neuroprotective effect	573:598	the neuroprotective effect	573:598	This study aimed to evaluate the neuroprotective effect and mechanism of dietary L edodes β-glucan supplementation against the obesity-associated cognitive decline in mice fed by a HF diet.
33541370	10	112	theme	occludin	1785:1792	arg1	expression					1748:1757	the expression	1744:1757	the expression of tight junction protein occludin	1744:1792	Furthermore, the β-glucan supplementation increased the mucosal thickness, upregulated the expression of tight junction protein occludin, decreased the plasma LPS level, and inhibited the proinflammatory macrophage accumulation in the colon of mice fed by HF diet.
33541370	2	113	dep	edodes	348:353	arg1	Lentinula					338:346	Lentinula	338:346	Lentinula	338:346	(1, 3)/(1, 6)-β-glucan, an edible polysaccharide isolated from medical mushroom, Lentinula edodes (L. edodes), has the potential to remodel gut microbiota.
33541370	8	114	dep	tests	1361:1365	arg1	tests					1361:1365	behavioral tests	1350:1365	behavioral tests (the temporal order memory, novel object recognition and Y-maze tests)	1350:1436	Long-term L. edodes β-glucan supplementation prevented the HF diet-induced recognition memory impairment assessed by behavioral tests (the temporal order memory, novel object recognition and Y-maze tests).
33541370	8	114	dep	tests	1361:1365	arg1	recognition					1408:1418	novel object recognition	1395:1418	novel object recognition	1395:1418	Long-term L. edodes β-glucan supplementation prevented the HF diet-induced recognition memory impairment assessed by behavioral tests (the temporal order memory, novel object recognition and Y-maze tests).
33541370	8	114	dep	tests	1361:1365	arg1	tests					1431:1435	Y-maze tests	1424:1435	Y-maze tests	1424:1435	Long-term L. edodes β-glucan supplementation prevented the HF diet-induced recognition memory impairment assessed by behavioral tests (the temporal order memory, novel object recognition and Y-maze tests).
33541370	8	114	dep	tests	1361:1365	arg1	memory					1387:1392	the temporal order memory	1368:1392	the temporal order memory	1368:1392	Long-term L. edodes β-glucan supplementation prevented the HF diet-induced recognition memory impairment assessed by behavioral tests (the temporal order memory, novel object recognition and Y-maze tests).
33541370	8	115	theme	HF	1292:1293	arg1	impairment					1327:1336	the HF diet-induced recognition memory impairment	1288:1336	the HF diet-induced recognition memory impairment assessed by behavioral tests (the temporal order memory, novel object recognition and Y-maze tests)	1288:1436	Long-term L. edodes β-glucan supplementation prevented the HF diet-induced recognition memory impairment assessed by behavioral tests (the temporal order memory, novel object recognition and Y-maze tests).
33541370	7	116	theme	edodes	1131:1136	arg1	supplementation					1147:1161	short-term and long-term L. edodes β-glucan supplementation	1103:1161	short-term and long-term L. edodes β-glucan supplementation	1103:1161	RESULTS We reported that short-term and long-term L. edodes β-glucan supplementation prevented the gut microbial composition shift induced by the HF diet.
33541370	10	117	theme	plasma	1809:1814	arg1	level					1820:1824	the plasma LPS level	1805:1824	the plasma LPS level	1805:1824	Furthermore, the β-glucan supplementation increased the mucosal thickness, upregulated the expression of tight junction protein occludin, decreased the plasma LPS level, and inhibited the proinflammatory macrophage accumulation in the colon of mice fed by HF diet.
33541370	8	118	theme	recognition	1308:1318	arg1	impairment					1327:1336	the HF diet-induced recognition memory impairment	1288:1336	the HF diet-induced recognition memory impairment assessed by behavioral tests (the temporal order memory, novel object recognition and Y-maze tests)	1288:1436	Long-term L. edodes β-glucan supplementation prevented the HF diet-induced recognition memory impairment assessed by behavioral tests (the temporal order memory, novel object recognition and Y-maze tests).
33541370	5	119	theme	lab	784:786	arg1	LC					794:795	LC	794:795	LC	794:795	METHODS C57BL/6J male mice were fed with either a lab chow (LC), HF or HF with L. edodes β-glucan supplementation diets for 7 days (short-term) or 15 weeks (long-term).
33541370	5	119	theme	lab	784:786	arg1	chow					788:791	a lab chow	782:791	a lab chow (LC)	782:796	METHODS C57BL/6J male mice were fed with either a lab chow (LC), HF or HF with L. edodes β-glucan supplementation diets for 7 days (short-term) or 15 weeks (long-term).
33541370	7	120	theme	long-term	1118:1126	arg1	supplementation					1147:1161	short-term and long-term L. edodes β-glucan supplementation	1103:1161	short-term and long-term L. edodes β-glucan supplementation	1103:1161	RESULTS We reported that short-term and long-term L. edodes β-glucan supplementation prevented the gut microbial composition shift induced by the HF diet.
33541370	1	121	theme	gut-brain	241:249	arg1	axis					251:254	the gut-brain axis	237:254	the gut-brain axis	237:254	BACKGROUND Long-term high fat (HF) diet intake can cause neuroinflammation and cognitive decline through the gut-brain axis.
34863833	0	0	theme	antibacterial	89:101	arg1	aerogels					21:28	aerogels	21:28	aerogels functionalized with AgO: Preparation, characterization and antibacterial activity	21:110	Cellulose nanofibers aerogels functionalized with AgO: Preparation, characterization and antibacterial activity.
34863833	0	0	theme	antibacterial	89:101	arg1	activity					103:110	antibacterial activity	89:110	antibacterial activity	89:110	Cellulose nanofibers aerogels functionalized with AgO: Preparation, characterization and antibacterial activity.
34863833	2	1	contain	has	337:339	arg1	oxide					331:335	nano‑silver oxide	319:335	nano‑silver oxide	319:335	Compared with silver nanoparticles and monovalent silver compounds, nano‑silver oxide has better antibacterial properties.
34863833	2	1	contain	has	337:339	arg2	properties					362:371	better antibacterial properties	341:371	better antibacterial properties	341:371	Compared with silver nanoparticles and monovalent silver compounds, nano‑silver oxide has better antibacterial properties.
34863833	6	2	theme	antibacterial	1095:1107	arg1	activities					1109:1118	significant antibacterial activities	1083:1118	significant antibacterial activities	1083:1118	The diameter of the inhibition zone of the aerogel against Escherichia coli and Staphylococcus aureus was 23 mm and 20 mm respectively, and the aerogels still exhibited significant antibacterial activities with more than 99.5% reductions in Escherichia coli and Staphylococcus aureus, which shows highly effective antibacterial properties.
34863833	1	3	theme	10 nm	244:248	arg1	size					230:233	a particle size	219:233	a particle size of about 10 nm	219:248	In the experiment, a chemical oxidation method was used to prepare nano-divalent silver oxide powder with a particle size of about 10 nm.
34863833	7	4	theme	high	1392:1395	arg1	efficiency					1397:1406	high efficiency	1392:1406	high efficiency	1392:1406	This research proposes an economical and novel preparation method of antibacterial cellulose aerogel, making it a candidate material with high efficiency, broad-spectrum antibacterial and more suitable for life needs.
34863833	4	5	dep	in	573:574	arg1	vitro					576:580	vitro	576:580	vitro	576:580	The microscopic morphology, mechanical properties, in vitro release of silver ions, antibacterial properties and biodegradability of composite aerogels were studied.
34863833	6	6	theme	significant	1083:1093	arg1	activities					1109:1118	significant antibacterial activities	1083:1118	significant antibacterial activities	1083:1118	The diameter of the inhibition zone of the aerogel against Escherichia coli and Staphylococcus aureus was 23 mm and 20 mm respectively, and the aerogels still exhibited significant antibacterial activities with more than 99.5% reductions in Escherichia coli and Staphylococcus aureus, which shows highly effective antibacterial properties.
34863833	4	7	theme	antibacterial	606:618	arg1	properties					620:629	antibacterial properties	606:629	antibacterial properties	606:629	The microscopic morphology, mechanical properties, in vitro release of silver ions, antibacterial properties and biodegradability of composite aerogels were studied.
34863833	4	8	theme	mechanical	550:559	arg1	properties					561:570	mechanical properties	550:570	mechanical properties	550:570	The microscopic morphology, mechanical properties, in vitro release of silver ions, antibacterial properties and biodegradability of composite aerogels were studied.
34863833	5	9	theme	cellulose	708:716	arg1	aerogel					732:738	the cellulose antibacterial aerogel	704:738	the cellulose antibacterial aerogel	704:738	The porosity of the cellulose antibacterial aerogel can reach 94%, the swelling rate was greater than 1000%, and the pore size was between 13 and 15 nm, which showed a larger storage space and attachment site for the aerogel.
34863833	1	10	used	used	164:167	arg2	method					153:158	a chemical oxidation method	132:158	a chemical oxidation method	132:158	In the experiment, a chemical oxidation method was used to prepare nano-divalent silver oxide powder with a particle size of about 10 nm.
34863833	3	11	theme	cellulose	378:386	arg1	aerogel					402:408	The cellulose antibacterial aerogel	374:408	The cellulose antibacterial aerogel	374:408	The cellulose antibacterial aerogel was prepared by combining it with cellulose nanofibrils and using freeze-thaw cycles and freeze-drying methods.
34863833	5	12	theme	antibacterial	718:730	arg1	aerogel					732:738	the cellulose antibacterial aerogel	704:738	the cellulose antibacterial aerogel	704:738	The porosity of the cellulose antibacterial aerogel can reach 94%, the swelling rate was greater than 1000%, and the pore size was between 13 and 15 nm, which showed a larger storage space and attachment site for the aerogel.
34863833	5	13	dep	%	752:752	arg1	%					794:794	greater than 1000%	777:794	greater than 1000%	777:794	The porosity of the cellulose antibacterial aerogel can reach 94%, the swelling rate was greater than 1000%, and the pore size was between 13 and 15 nm, which showed a larger storage space and attachment site for the aerogel.
34863833	5	13	dep	%	752:752	arg1	rate					768:771	the swelling rate	755:771	the swelling rate	755:771	The porosity of the cellulose antibacterial aerogel can reach 94%, the swelling rate was greater than 1000%, and the pore size was between 13 and 15 nm, which showed a larger storage space and attachment site for the aerogel.
34863833	5	13	dep	%	752:752	arg1	13					827:828	13	827:828	13	827:828	The porosity of the cellulose antibacterial aerogel can reach 94%, the swelling rate was greater than 1000%, and the pore size was between 13 and 15 nm, which showed a larger storage space and attachment site for the aerogel.
34863833	7	14	theme	novel	1295:1299	arg1	method					1313:1318	an economical and novel preparation method	1277:1318	an economical and novel preparation method of antibacterial cellulose aerogel	1277:1353	This research proposes an economical and novel preparation method of antibacterial cellulose aerogel, making it a candidate material with high efficiency, broad-spectrum antibacterial and more suitable for life needs.
34863833	7	15	theme	preparation	1301:1311	arg1	method					1313:1318	an economical and novel preparation method	1277:1318	an economical and novel preparation method of antibacterial cellulose aerogel	1277:1353	This research proposes an economical and novel preparation method of antibacterial cellulose aerogel, making it a candidate material with high efficiency, broad-spectrum antibacterial and more suitable for life needs.
34863833	7	16	theme	aerogel	1347:1353	arg1	method					1313:1318	an economical and novel preparation method	1277:1318	an economical and novel preparation method of antibacterial cellulose aerogel	1277:1353	This research proposes an economical and novel preparation method of antibacterial cellulose aerogel, making it a candidate material with high efficiency, broad-spectrum antibacterial and more suitable for life needs.
34863833	3	17	theme	cellulose	444:452	arg1	nanofibrils					454:464	cellulose nanofibrils	444:464	cellulose nanofibrils	444:464	The cellulose antibacterial aerogel was prepared by combining it with cellulose nanofibrils and using freeze-thaw cycles and freeze-drying methods.
34863833	5	18	theme	larger	856:861	arg1	space					871:875	a larger storage space and attachment site	854:895	space	871:875	The porosity of the cellulose antibacterial aerogel can reach 94%, the swelling rate was greater than 1000%, and the pore size was between 13 and 15 nm, which showed a larger storage space and attachment site for the aerogel.
34863833	4	19	theme	microscopic	526:536	arg1	morphology					538:547	The microscopic morphology	522:547	The microscopic morphology	522:547	The microscopic morphology, mechanical properties, in vitro release of silver ions, antibacterial properties and biodegradability of composite aerogels were studied.
34863833	2	20	theme	silver	265:270	arg1	nanoparticles					272:284	silver nanoparticles	265:284	silver nanoparticles	265:284	Compared with silver nanoparticles and monovalent silver compounds, nano‑silver oxide has better antibacterial properties.
34863833	5	21	theme	storage	863:869	arg1	space					871:875	a larger storage space and attachment site	854:895	space	871:875	The porosity of the cellulose antibacterial aerogel can reach 94%, the swelling rate was greater than 1000%, and the pore size was between 13 and 15 nm, which showed a larger storage space and attachment site for the aerogel.
34863833	2	22	theme	monovalent	290:299	arg1	compounds					308:316	monovalent silver compounds	290:316	monovalent silver compounds	290:316	Compared with silver nanoparticles and monovalent silver compounds, nano‑silver oxide has better antibacterial properties.
34863833	2	23	theme	nano‑silver	319:329	arg1	oxide					331:335	nano‑silver oxide	319:335	nano‑silver oxide	319:335	Compared with silver nanoparticles and monovalent silver compounds, nano‑silver oxide has better antibacterial properties.
34863833	1	24	theme	nano-divalent	180:192	arg1	powder					207:212	nano-divalent silver oxide powder	180:212	nano-divalent silver oxide powder	180:212	In the experiment, a chemical oxidation method was used to prepare nano-divalent silver oxide powder with a particle size of about 10 nm.
34863833	4	25	theme	silver	593:598	arg1	ions					600:603	silver ions	593:603	silver ions	593:603	The microscopic morphology, mechanical properties, in vitro release of silver ions, antibacterial properties and biodegradability of composite aerogels were studied.
34863833	1	26	theme	silver	194:199	arg1	powder					207:212	nano-divalent silver oxide powder	180:212	nano-divalent silver oxide powder	180:212	In the experiment, a chemical oxidation method was used to prepare nano-divalent silver oxide powder with a particle size of about 10 nm.
34863833	3	27	theme	antibacterial	388:400	arg1	aerogel					402:408	The cellulose antibacterial aerogel	374:408	The cellulose antibacterial aerogel	374:408	The cellulose antibacterial aerogel was prepared by combining it with cellulose nanofibrils and using freeze-thaw cycles and freeze-drying methods.
34863833	6	28	theme	aerogel	957:963	arg1	zone					945:948	the inhibition zone	930:948	the inhibition zone of the aerogel against Escherichia coli and Staphylococcus aureus	930:1014	The diameter of the inhibition zone of the aerogel against Escherichia coli and Staphylococcus aureus was 23 mm and 20 mm respectively, and the aerogels still exhibited significant antibacterial activities with more than 99.5% reductions in Escherichia coli and Staphylococcus aureus, which shows highly effective antibacterial properties.
34863833	7	29	dep	life	1460:1463	arg1	needs					1465:1469	needs	1465:1469	needs	1465:1469	This research proposes an economical and novel preparation method of antibacterial cellulose aerogel, making it a candidate material with high efficiency, broad-spectrum antibacterial and more suitable for life needs.
34863833	6	30	from	reductions	1141:1150	arg1	coli					1167:1170	Escherichia coli	1155:1170	Escherichia coli	1155:1170	The diameter of the inhibition zone of the aerogel against Escherichia coli and Staphylococcus aureus was 23 mm and 20 mm respectively, and the aerogels still exhibited significant antibacterial activities with more than 99.5% reductions in Escherichia coli and Staphylococcus aureus, which shows highly effective antibacterial properties.
34863833	6	30	from	reductions	1141:1150	arg1	aureus					1191:1196	Staphylococcus aureus	1176:1196	Staphylococcus aureus	1176:1196	The diameter of the inhibition zone of the aerogel against Escherichia coli and Staphylococcus aureus was 23 mm and 20 mm respectively, and the aerogels still exhibited significant antibacterial activities with more than 99.5% reductions in Escherichia coli and Staphylococcus aureus, which shows highly effective antibacterial properties.
34863833	1	31	theme	oxide	201:205	arg1	powder					207:212	nano-divalent silver oxide powder	180:212	nano-divalent silver oxide powder	180:212	In the experiment, a chemical oxidation method was used to prepare nano-divalent silver oxide powder with a particle size of about 10 nm.
34863833	2	32	theme	silver	301:306	arg1	compounds					308:316	monovalent silver compounds	290:316	monovalent silver compounds	290:316	Compared with silver nanoparticles and monovalent silver compounds, nano‑silver oxide has better antibacterial properties.
34863833	3	33	theme	freeze-drying	499:511	arg1	methods					513:519	freeze-drying methods	499:519	freeze-drying methods	499:519	The cellulose antibacterial aerogel was prepared by combining it with cellulose nanofibrils and using freeze-thaw cycles and freeze-drying methods.
34863833	4	34	theme	ions	600:603	arg1	biodegradability					635:650	biodegradability	635:650	biodegradability	635:650	The microscopic morphology, mechanical properties, in vitro release of silver ions, antibacterial properties and biodegradability of composite aerogels were studied.
34863833	4	34	theme	ions	600:603	arg1	release					582:588	in vitro release	573:588	in vitro release of silver ions	573:603	The microscopic morphology, mechanical properties, in vitro release of silver ions, antibacterial properties and biodegradability of composite aerogels were studied.
34863833	4	34	theme	ions	600:603	arg1	properties					620:629	antibacterial properties	606:629	antibacterial properties	606:629	The microscopic morphology, mechanical properties, in vitro release of silver ions, antibacterial properties and biodegradability of composite aerogels were studied.
34863833	4	34	theme	ions	600:603	arg1	morphology					538:547	The microscopic morphology	522:547	The microscopic morphology	522:547	The microscopic morphology, mechanical properties, in vitro release of silver ions, antibacterial properties and biodegradability of composite aerogels were studied.
34863833	4	34	theme	ions	600:603	arg1	properties					561:570	mechanical properties	550:570	mechanical properties	550:570	The microscopic morphology, mechanical properties, in vitro release of silver ions, antibacterial properties and biodegradability of composite aerogels were studied.
34863833	7	35	theme	broad-spectrum	1409:1422	arg1	antibacterial					1424:1436	broad-spectrum antibacterial	1409:1436	broad-spectrum antibacterial	1409:1436	This research proposes an economical and novel preparation method of antibacterial cellulose aerogel, making it a candidate material with high efficiency, broad-spectrum antibacterial and more suitable for life needs.
34863833	3	36	theme	freeze-thaw	476:486	arg1	cycles					488:493	freeze-thaw cycles	476:493	freeze-thaw cycles	476:493	The cellulose antibacterial aerogel was prepared by combining it with cellulose nanofibrils and using freeze-thaw cycles and freeze-drying methods.
34863833	5	37	theme	pore	805:808	arg1	size					810:813	the pore size	801:813	the pore size	801:813	The porosity of the cellulose antibacterial aerogel can reach 94%, the swelling rate was greater than 1000%, and the pore size was between 13 and 15 nm, which showed a larger storage space and attachment site for the aerogel.
34863833	4	38	theme	in	573:574	arg1	release					582:588	in vitro release	573:588	in vitro release of silver ions	573:603	The microscopic morphology, mechanical properties, in vitro release of silver ions, antibacterial properties and biodegradability of composite aerogels were studied.
34863833	5	39	theme	swelling	759:766	arg1	%					794:794	greater than 1000%	777:794	greater than 1000%	777:794	The porosity of the cellulose antibacterial aerogel can reach 94%, the swelling rate was greater than 1000%, and the pore size was between 13 and 15 nm, which showed a larger storage space and attachment site for the aerogel.
34863833	5	39	theme	swelling	759:766	arg1	rate					768:771	the swelling rate	755:771	the swelling rate	755:771	The porosity of the cellulose antibacterial aerogel can reach 94%, the swelling rate was greater than 1000%, and the pore size was between 13 and 15 nm, which showed a larger storage space and attachment site for the aerogel.
34863833	7	40	theme	antibacterial	1323:1335	arg1	aerogel					1347:1353	antibacterial cellulose aerogel	1323:1353	antibacterial cellulose aerogel	1323:1353	This research proposes an economical and novel preparation method of antibacterial cellulose aerogel, making it a candidate material with high efficiency, broad-spectrum antibacterial and more suitable for life needs.
34863833	5	41	theme	attachment	881:890	arg1	site					892:895	a larger storage space and attachment site	854:895	site	892:895	The porosity of the cellulose antibacterial aerogel can reach 94%, the swelling rate was greater than 1000%, and the pore size was between 13 and 15 nm, which showed a larger storage space and attachment site for the aerogel.
34863833	5	42	theme	aerogel	732:738	arg1	porosity					692:699	The porosity	688:699	The porosity of the cellulose antibacterial aerogel	688:738	The porosity of the cellulose antibacterial aerogel can reach 94%, the swelling rate was greater than 1000%, and the pore size was between 13 and 15 nm, which showed a larger storage space and attachment site for the aerogel.
34863833	2	43	theme	antibacterial	348:360	arg1	properties					362:371	better antibacterial properties	341:371	better antibacterial properties	341:371	Compared with silver nanoparticles and monovalent silver compounds, nano‑silver oxide has better antibacterial properties.
34863833	6	44	theme	antibacterial	1228:1240	arg1	properties					1242:1251	highly effective antibacterial properties	1211:1251	highly effective antibacterial properties	1211:1251	The diameter of the inhibition zone of the aerogel against Escherichia coli and Staphylococcus aureus was 23 mm and 20 mm respectively, and the aerogels still exhibited significant antibacterial activities with more than 99.5% reductions in Escherichia coli and Staphylococcus aureus, which shows highly effective antibacterial properties.
34863833	2	45	theme	better	341:346	arg1	properties					362:371	better antibacterial properties	341:371	better antibacterial properties	341:371	Compared with silver nanoparticles and monovalent silver compounds, nano‑silver oxide has better antibacterial properties.
34863833	7	46	theme	economical	1280:1289	arg1	method					1313:1318	an economical and novel preparation method	1277:1318	an economical and novel preparation method of antibacterial cellulose aerogel	1277:1353	This research proposes an economical and novel preparation method of antibacterial cellulose aerogel, making it a candidate material with high efficiency, broad-spectrum antibacterial and more suitable for life needs.
34863833	6	47	theme	zone	945:948	arg1	23 mm					1020:1024	23 mm	1020:1024	23 mm	1020:1024	The diameter of the inhibition zone of the aerogel against Escherichia coli and Staphylococcus aureus was 23 mm and 20 mm respectively, and the aerogels still exhibited significant antibacterial activities with more than 99.5% reductions in Escherichia coli and Staphylococcus aureus, which shows highly effective antibacterial properties.
34863833	6	47	theme	zone	945:948	arg1	diameter					918:925	The diameter	914:925	The diameter of the inhibition zone of the aerogel against Escherichia coli and Staphylococcus aureus	914:1014	The diameter of the inhibition zone of the aerogel against Escherichia coli and Staphylococcus aureus was 23 mm and 20 mm respectively, and the aerogels still exhibited significant antibacterial activities with more than 99.5% reductions in Escherichia coli and Staphylococcus aureus, which shows highly effective antibacterial properties.
34863833	6	48	theme	effective	1218:1226	arg1	properties					1242:1251	highly effective antibacterial properties	1211:1251	highly effective antibacterial properties	1211:1251	The diameter of the inhibition zone of the aerogel against Escherichia coli and Staphylococcus aureus was 23 mm and 20 mm respectively, and the aerogels still exhibited significant antibacterial activities with more than 99.5% reductions in Escherichia coli and Staphylococcus aureus, which shows highly effective antibacterial properties.
34863833	1	49	theme	particle	221:228	arg1	size					230:233	a particle size	219:233	a particle size of about 10 nm	219:248	In the experiment, a chemical oxidation method was used to prepare nano-divalent silver oxide powder with a particle size of about 10 nm.
34863833	0	50	dep	aerogels	21:28	arg1	aerogels					21:28	aerogels	21:28	aerogels functionalized with AgO: Preparation, characterization and antibacterial activity	21:110	Cellulose nanofibers aerogels functionalized with AgO: Preparation, characterization and antibacterial activity.
34863833	0	50	dep	aerogels	21:28	arg1	characterization					68:83	characterization	68:83	characterization	68:83	Cellulose nanofibers aerogels functionalized with AgO: Preparation, characterization and antibacterial activity.
34863833	0	50	dep	aerogels	21:28	arg1	activity					103:110	antibacterial activity	89:110	antibacterial activity	89:110	Cellulose nanofibers aerogels functionalized with AgO: Preparation, characterization and antibacterial activity.
34863833	0	50	dep	aerogels	21:28	arg1	Preparation					55:65	Preparation	55:65	Preparation	55:65	Cellulose nanofibers aerogels functionalized with AgO: Preparation, characterization and antibacterial activity.
34863833	1	51	theme	chemical	134:141	arg1	method					153:158	a chemical oxidation method	132:158	a chemical oxidation method	132:158	In the experiment, a chemical oxidation method was used to prepare nano-divalent silver oxide powder with a particle size of about 10 nm.
34863833	6	52	dep	%	1139:1139	arg1	99.5					1135:1138	99.5	1135:1138	99.5	1135:1138	The diameter of the inhibition zone of the aerogel against Escherichia coli and Staphylococcus aureus was 23 mm and 20 mm respectively, and the aerogels still exhibited significant antibacterial activities with more than 99.5% reductions in Escherichia coli and Staphylococcus aureus, which shows highly effective antibacterial properties.
34863833	7	53	theme	cellulose	1337:1345	arg1	aerogel					1347:1353	antibacterial cellulose aerogel	1323:1353	antibacterial cellulose aerogel	1323:1353	This research proposes an economical and novel preparation method of antibacterial cellulose aerogel, making it a candidate material with high efficiency, broad-spectrum antibacterial and more suitable for life needs.
34863833	1	54	theme	oxidation	143:151	arg1	method					153:158	a chemical oxidation method	132:158	a chemical oxidation method	132:158	In the experiment, a chemical oxidation method was used to prepare nano-divalent silver oxide powder with a particle size of about 10 nm.
34863833	6	55	dep	reductions	1141:1150	arg1	%					1139:1139	%	1139:1139	%	1139:1139	The diameter of the inhibition zone of the aerogel against Escherichia coli and Staphylococcus aureus was 23 mm and 20 mm respectively, and the aerogels still exhibited significant antibacterial activities with more than 99.5% reductions in Escherichia coli and Staphylococcus aureus, which shows highly effective antibacterial properties.
34863833	7	56	with	material	1378:1385	arg1	suitable					1447:1454	suitable	1447:1454	suitable	1447:1454	This research proposes an economical and novel preparation method of antibacterial cellulose aerogel, making it a candidate material with high efficiency, broad-spectrum antibacterial and more suitable for life needs.
34863833	7	56	with	material	1378:1385	arg1	efficiency					1397:1406	high efficiency	1392:1406	high efficiency	1392:1406	This research proposes an economical and novel preparation method of antibacterial cellulose aerogel, making it a candidate material with high efficiency, broad-spectrum antibacterial and more suitable for life needs.
34863833	7	56	with	material	1378:1385	arg1	antibacterial					1424:1436	broad-spectrum antibacterial	1409:1436	broad-spectrum antibacterial	1409:1436	This research proposes an economical and novel preparation method of antibacterial cellulose aerogel, making it a candidate material with high efficiency, broad-spectrum antibacterial and more suitable for life needs.
34863833	7	57	theme	material	1378:1385	arg1	candidate					1368:1376	a candidate	1366:1376	a candidate material with high efficiency, broad-spectrum antibacterial and more suitable for life needs	1366:1469	This research proposes an economical and novel preparation method of antibacterial cellulose aerogel, making it a candidate material with high efficiency, broad-spectrum antibacterial and more suitable for life needs.
34863833	4	58	theme	composite	655:663	arg1	aerogels					665:672	composite aerogels	655:672	composite aerogels	655:672	The microscopic morphology, mechanical properties, in vitro release of silver ions, antibacterial properties and biodegradability of composite aerogels were studied.
34863833	6	59	theme	inhibition	934:943	arg1	zone					945:948	the inhibition zone	930:948	the inhibition zone of the aerogel against Escherichia coli and Staphylococcus aureus	930:1014	The diameter of the inhibition zone of the aerogel against Escherichia coli and Staphylococcus aureus was 23 mm and 20 mm respectively, and the aerogels still exhibited significant antibacterial activities with more than 99.5% reductions in Escherichia coli and Staphylococcus aureus, which shows highly effective antibacterial properties.
34863833	4	60	theme	aerogels	665:672	arg1	biodegradability					635:650	biodegradability	635:650	biodegradability	635:650	The microscopic morphology, mechanical properties, in vitro release of silver ions, antibacterial properties and biodegradability of composite aerogels were studied.
34863833	4	60	theme	aerogels	665:672	arg1	release					582:588	in vitro release	573:588	in vitro release of silver ions	573:603	The microscopic morphology, mechanical properties, in vitro release of silver ions, antibacterial properties and biodegradability of composite aerogels were studied.
34863833	4	60	theme	aerogels	665:672	arg1	properties					620:629	antibacterial properties	606:629	antibacterial properties	606:629	The microscopic morphology, mechanical properties, in vitro release of silver ions, antibacterial properties and biodegradability of composite aerogels were studied.
34863833	4	60	theme	aerogels	665:672	arg1	morphology					538:547	The microscopic morphology	522:547	The microscopic morphology	522:547	The microscopic morphology, mechanical properties, in vitro release of silver ions, antibacterial properties and biodegradability of composite aerogels were studied.
34863833	4	60	theme	aerogels	665:672	arg1	properties					561:570	mechanical properties	550:570	mechanical properties	550:570	The microscopic morphology, mechanical properties, in vitro release of silver ions, antibacterial properties and biodegradability of composite aerogels were studied.
33545643	1	0	theme	materials	344:352	arg1	processes					316:324	the preparation processes	300:324	the preparation processes of food packaging materials, therefore, an effective encapsulation of LEO	300:398	Lemon essential oils (LEO), as natural bacteriostatic agents, show significant loss in the preparation processes of food packaging materials, therefore, an effective encapsulation of LEO is urgent for realizing the protection.
33545643	2	1	theme	konjac	576:581	arg1	glucomannan-grafted-poly					583:606	konjac glucomannan-grafted-poly	576:606	konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol (KGM-g-PAA/PVA) composite	576:665	In this study, LEO was absorbed by thermally stable and porous vermiculite (VML) to form LEO/VML complex, which is further coupled with konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol (KGM-g-PAA/PVA) composite.
33545643	2	1	theme	konjac	576:581	arg1	acid					617:620	acrylic acid	609:620	acrylic acid	609:620	In this study, LEO was absorbed by thermally stable and porous vermiculite (VML) to form LEO/VML complex, which is further coupled with konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol (KGM-g-PAA/PVA) composite.
33545643	1	2	theme	bacteriostatic	252:265	arg1	agents					267:272	natural bacteriostatic agents	244:272	natural bacteriostatic agents	244:272	Lemon essential oils (LEO), as natural bacteriostatic agents, show significant loss in the preparation processes of food packaging materials, therefore, an effective encapsulation of LEO is urgent for realizing the protection.
33545643	4	3	theme	law	959:961	arg1	mechanism					931:939	the predominant mechanism	915:939	the predominant mechanism of Fick diffusion law	915:961	The VML (1 g) can significantly reduce LEO loss and achieve sustained control LEO release from the pad, which follows the predominant mechanism of Fick diffusion law.
33545643	3	4	theme	KGM-g-PAA/PVA	668:680	arg1	pad					713:715	KGM-g-PAA/PVA bacteriostatic water-absorbing pad	668:715	KGM-g-PAA/PVA bacteriostatic water-absorbing pad	668:715	KGM-g-PAA/PVA bacteriostatic water-absorbing pad was prepared via electrospinning technique, which can minimize the loss of LEO.
33545643	0	5	theme	food	194:197	arg1	preservation					199:210	food preservation	194:210	food preservation	194:210	Lemon essential oil/vermiculite encapsulated in electrospun konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol bacteriostatic pad: Sustained control release and its application in food preservation.
33545643	4	6	from	pad	896:898	arg1	release					879:885	sustained control LEO release	857:885	sustained control LEO release from the pad, which follows the predominant mechanism of Fick diffusion law	857:961	The VML (1 g) can significantly reduce LEO loss and achieve sustained control LEO release from the pad, which follows the predominant mechanism of Fick diffusion law.
33545643	0	7	from	application	179:189	arg1	preservation					199:210	food preservation	194:210	food preservation	194:210	Lemon essential oil/vermiculite encapsulated in electrospun konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol bacteriostatic pad: Sustained control release and its application in food preservation.
33545643	0	8	theme	alcohol	117:123	arg1	pad					140:142	/polyvinyl alcohol bacteriostatic pad	106:142	/polyvinyl alcohol bacteriostatic pad	106:142	Lemon essential oil/vermiculite encapsulated in electrospun konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol bacteriostatic pad: Sustained control release and its application in food preservation.
33545643	3	9	theme	bacteriostatic	682:695	arg1	pad					713:715	KGM-g-PAA/PVA bacteriostatic water-absorbing pad	668:715	KGM-g-PAA/PVA bacteriostatic water-absorbing pad	668:715	KGM-g-PAA/PVA bacteriostatic water-absorbing pad was prepared via electrospinning technique, which can minimize the loss of LEO.
33545643	4	10	theme	control	867:873	arg1	release					879:885	sustained control LEO release	857:885	sustained control LEO release from the pad, which follows the predominant mechanism of Fick diffusion law	857:961	The VML (1 g) can significantly reduce LEO loss and achieve sustained control LEO release from the pad, which follows the predominant mechanism of Fick diffusion law.
33545643	0	11	theme	/polyvinyl	106:115	arg1	pad					140:142	/polyvinyl alcohol bacteriostatic pad	106:142	/polyvinyl alcohol bacteriostatic pad	106:142	Lemon essential oil/vermiculite encapsulated in electrospun konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol bacteriostatic pad: Sustained control release and its application in food preservation.
33545643	4	12	theme	sustained	857:865	arg1	release					879:885	sustained control LEO release	857:885	sustained control LEO release from the pad, which follows the predominant mechanism of Fick diffusion law	857:961	The VML (1 g) can significantly reduce LEO loss and achieve sustained control LEO release from the pad, which follows the predominant mechanism of Fick diffusion law.
33545643	6	13	theme	intelligent	1213:1223	arg1	packaging					1225:1233	intelligent packaging	1213:1233	intelligent packaging	1213:1233	This study suggests that KGM-g-PAA/PVA pad may have a great potential in the field of intelligent packaging.
33545643	5	14	theme	coli	1052:1055	arg1	growth					1039:1044	the growth	1035:1044	the growth of E. coli	1035:1055	The sustained control LEO release from the pad can effectively inhibit the growth of E. coli during storage, thus prolonging shelf life of chilled pork for 3 day.
33545643	0	15	theme	bacteriostatic	125:138	arg1	pad					140:142	/polyvinyl alcohol bacteriostatic pad	106:142	/polyvinyl alcohol bacteriostatic pad	106:142	Lemon essential oil/vermiculite encapsulated in electrospun konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol bacteriostatic pad: Sustained control release and its application in food preservation.
33545643	1	16	theme	effective	369:377	arg1	materials					344:352	food packaging materials	329:352	food packaging materials	329:352	Lemon essential oils (LEO), as natural bacteriostatic agents, show significant loss in the preparation processes of food packaging materials, therefore, an effective encapsulation of LEO is urgent for realizing the protection.
33545643	1	16	theme	effective	369:377	arg1	encapsulation					379:391	an effective encapsulation	366:391	an effective encapsulation of LEO	366:398	Lemon essential oils (LEO), as natural bacteriostatic agents, show significant loss in the preparation processes of food packaging materials, therefore, an effective encapsulation of LEO is urgent for realizing the protection.
33545643	2	17	theme	acrylic	609:615	arg1	glucomannan-grafted-poly					583:606	konjac glucomannan-grafted-poly	576:606	konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol (KGM-g-PAA/PVA) composite	576:665	In this study, LEO was absorbed by thermally stable and porous vermiculite (VML) to form LEO/VML complex, which is further coupled with konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol (KGM-g-PAA/PVA) composite.
33545643	2	17	theme	acrylic	609:615	arg1	acid					617:620	acrylic acid	609:620	acrylic acid	609:620	In this study, LEO was absorbed by thermally stable and porous vermiculite (VML) to form LEO/VML complex, which is further coupled with konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol (KGM-g-PAA/PVA) composite.
33545643	0	18	theme	essential	6:14	arg1	oil/vermiculite					16:30	Lemon essential oil/vermiculite	0:30	Lemon essential oil/vermiculite	0:30	Lemon essential oil/vermiculite encapsulated in electrospun konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol bacteriostatic pad: Sustained control release and its application in food preservation.
33545643	2	19	theme	composite	657:665	arg1	glucomannan-grafted-poly					583:606	konjac glucomannan-grafted-poly	576:606	konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol (KGM-g-PAA/PVA) composite	576:665	In this study, LEO was absorbed by thermally stable and porous vermiculite (VML) to form LEO/VML complex, which is further coupled with konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol (KGM-g-PAA/PVA) composite.
33545643	2	19	theme	composite	657:665	arg1	acid					617:620	acrylic acid	609:620	acrylic acid	609:620	In this study, LEO was absorbed by thermally stable and porous vermiculite (VML) to form LEO/VML complex, which is further coupled with konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol (KGM-g-PAA/PVA) composite.
33545643	4	20	theme	LEO	875:877	arg1	release					879:885	sustained control LEO release	857:885	sustained control LEO release from the pad, which follows the predominant mechanism of Fick diffusion law	857:961	The VML (1 g) can significantly reduce LEO loss and achieve sustained control LEO release from the pad, which follows the predominant mechanism of Fick diffusion law.
33545643	0	21	theme	Lemon	0:4	arg1	oil/vermiculite					16:30	Lemon essential oil/vermiculite	0:30	Lemon essential oil/vermiculite	0:30	Lemon essential oil/vermiculite encapsulated in electrospun konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol bacteriostatic pad: Sustained control release and its application in food preservation.
33545643	1	22	theme	significant	280:290	arg1	loss					292:295	significant loss	280:295	significant loss in the preparation processes of food packaging materials, therefore, an effective encapsulation of LEO	280:398	Lemon essential oils (LEO), as natural bacteriostatic agents, show significant loss in the preparation processes of food packaging materials, therefore, an effective encapsulation of LEO is urgent for realizing the protection.
33545643	1	23	from	loss	292:295	arg1	processes					316:324	the preparation processes	300:324	the preparation processes of food packaging materials, therefore, an effective encapsulation of LEO	300:398	Lemon essential oils (LEO), as natural bacteriostatic agents, show significant loss in the preparation processes of food packaging materials, therefore, an effective encapsulation of LEO is urgent for realizing the protection.
33545643	1	24	theme	Lemon	213:217	arg1	oils					229:232	Lemon essential oils	213:232	Lemon essential oils (LEO)	213:238	Lemon essential oils (LEO), as natural bacteriostatic agents, show significant loss in the preparation processes of food packaging materials, therefore, an effective encapsulation of LEO is urgent for realizing the protection.
33545643	1	24	theme	Lemon	213:217	arg1	LEO					235:237	LEO	235:237	LEO	235:237	Lemon essential oils (LEO), as natural bacteriostatic agents, show significant loss in the preparation processes of food packaging materials, therefore, an effective encapsulation of LEO is urgent for realizing the protection.
33545643	6	25	theme	KGM-g-PAA/PVA	1152:1164	arg1	pad					1166:1168	KGM-g-PAA/PVA pad	1152:1168	KGM-g-PAA/PVA pad	1152:1168	This study suggests that KGM-g-PAA/PVA pad may have a great potential in the field of intelligent packaging.
33545643	0	26	theme	Sustained	145:153	arg1	release					163:169	Sustained control release	145:169	Sustained control release	145:169	Lemon essential oil/vermiculite encapsulated in electrospun konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol bacteriostatic pad: Sustained control release and its application in food preservation.
33545643	5	27	from	pad	1007:1009	arg1	release					990:996	The sustained control LEO release	964:996	The sustained control LEO release from the pad	964:1009	The sustained control LEO release from the pad can effectively inhibit the growth of E. coli during storage, thus prolonging shelf life of chilled pork for 3 day.
33545643	1	28	theme	essential	219:227	arg1	oils					229:232	Lemon essential oils	213:232	Lemon essential oils (LEO)	213:238	Lemon essential oils (LEO), as natural bacteriostatic agents, show significant loss in the preparation processes of food packaging materials, therefore, an effective encapsulation of LEO is urgent for realizing the protection.
33545643	1	28	theme	essential	219:227	arg1	LEO					235:237	LEO	235:237	LEO	235:237	Lemon essential oils (LEO), as natural bacteriostatic agents, show significant loss in the preparation processes of food packaging materials, therefore, an effective encapsulation of LEO is urgent for realizing the protection.
33545643	5	29	theme	shelf	1089:1093	arg1	pork					1111:1114	shelf life of chilled pork	1089:1114	shelf life of chilled pork for 3 day	1089:1124	The sustained control LEO release from the pad can effectively inhibit the growth of E. coli during storage, thus prolonging shelf life of chilled pork for 3 day.
33545643	4	30	theme	diffusion	949:957	arg1	law					959:961	Fick diffusion law	944:961	Fick diffusion law	944:961	The VML (1 g) can significantly reduce LEO loss and achieve sustained control LEO release from the pad, which follows the predominant mechanism of Fick diffusion law.
33545643	0	31	theme	electrospun	48:58	arg1	acid					101:104	acrylic acid	93:104	acrylic acid	93:104	Lemon essential oil/vermiculite encapsulated in electrospun konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol bacteriostatic pad: Sustained control release and its application in food preservation.
33545643	0	31	theme	electrospun	48:58	arg1	glucomannan-grafted-poly					67:90	electrospun konjac glucomannan-grafted-poly	48:90	electrospun konjac glucomannan-grafted-poly (acrylic acid)	48:105	Lemon essential oil/vermiculite encapsulated in electrospun konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol bacteriostatic pad: Sustained control release and its application in food preservation.
33545643	1	32	theme	LEO	396:398	arg1	materials					344:352	food packaging materials	329:352	food packaging materials	329:352	Lemon essential oils (LEO), as natural bacteriostatic agents, show significant loss in the preparation processes of food packaging materials, therefore, an effective encapsulation of LEO is urgent for realizing the protection.
33545643	1	32	theme	LEO	396:398	arg1	encapsulation					379:391	an effective encapsulation	366:391	an effective encapsulation of LEO	366:398	Lemon essential oils (LEO), as natural bacteriostatic agents, show significant loss in the preparation processes of food packaging materials, therefore, an effective encapsulation of LEO is urgent for realizing the protection.
33545643	5	33	theme	of	1100:1101	arg1	pork					1111:1114	shelf life of chilled pork	1089:1114	shelf life of chilled pork for 3 day	1089:1124	The sustained control LEO release from the pad can effectively inhibit the growth of E. coli during storage, thus prolonging shelf life of chilled pork for 3 day.
33545643	1	34	theme	preparation	304:314	arg1	processes					316:324	the preparation processes	300:324	the preparation processes of food packaging materials, therefore, an effective encapsulation of LEO	300:398	Lemon essential oils (LEO), as natural bacteriostatic agents, show significant loss in the preparation processes of food packaging materials, therefore, an effective encapsulation of LEO is urgent for realizing the protection.
33545643	0	35	theme	control	155:161	arg1	release					163:169	Sustained control release	145:169	Sustained control release	145:169	Lemon essential oil/vermiculite encapsulated in electrospun konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol bacteriostatic pad: Sustained control release and its application in food preservation.
33545643	6	36	theme	great	1181:1185	arg1	potential					1187:1195	a great potential	1179:1195	a great potential	1179:1195	This study suggests that KGM-g-PAA/PVA pad may have a great potential in the field of intelligent packaging.
33545643	2	37	theme	porous	496:501	arg1	vermiculite					503:513	thermally stable and porous vermiculite	475:513	thermally stable and porous vermiculite (VML)	475:519	In this study, LEO was absorbed by thermally stable and porous vermiculite (VML) to form LEO/VML complex, which is further coupled with konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol (KGM-g-PAA/PVA) composite.
33545643	2	37	theme	porous	496:501	arg1	VML					516:518	VML	516:518	VML	516:518	In this study, LEO was absorbed by thermally stable and porous vermiculite (VML) to form LEO/VML complex, which is further coupled with konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol (KGM-g-PAA/PVA) composite.
33545643	4	38	theme	predominant	919:929	arg1	mechanism					931:939	the predominant mechanism	915:939	the predominant mechanism of Fick diffusion law	915:961	The VML (1 g) can significantly reduce LEO loss and achieve sustained control LEO release from the pad, which follows the predominant mechanism of Fick diffusion law.
33545643	4	39	theme	Fick	944:947	arg1	law					959:961	Fick diffusion law	944:961	Fick diffusion law	944:961	The VML (1 g) can significantly reduce LEO loss and achieve sustained control LEO release from the pad, which follows the predominant mechanism of Fick diffusion law.
33545643	4	40	theme	LEO	836:838	arg1	loss					840:843	LEO loss	836:843	LEO loss	836:843	The VML (1 g) can significantly reduce LEO loss and achieve sustained control LEO release from the pad, which follows the predominant mechanism of Fick diffusion law.
33545643	5	41	theme	life	1095:1098	arg1	pork					1111:1114	shelf life of chilled pork	1089:1114	shelf life of chilled pork for 3 day	1089:1124	The sustained control LEO release from the pad can effectively inhibit the growth of E. coli during storage, thus prolonging shelf life of chilled pork for 3 day.
33545643	0	42	theme	konjac	60:65	arg1	acid					101:104	acrylic acid	93:104	acrylic acid	93:104	Lemon essential oil/vermiculite encapsulated in electrospun konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol bacteriostatic pad: Sustained control release and its application in food preservation.
33545643	0	42	theme	konjac	60:65	arg1	glucomannan-grafted-poly					67:90	electrospun konjac glucomannan-grafted-poly	48:90	electrospun konjac glucomannan-grafted-poly (acrylic acid)	48:105	Lemon essential oil/vermiculite encapsulated in electrospun konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol bacteriostatic pad: Sustained control release and its application in food preservation.
33545643	2	43	theme	LEO/VML	529:535	arg1	complex					537:543	LEO/VML complex	529:543	LEO/VML complex	529:543	In this study, LEO was absorbed by thermally stable and porous vermiculite (VML) to form LEO/VML complex, which is further coupled with konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol (KGM-g-PAA/PVA) composite.
33545643	5	44	theme	chilled	1103:1109	arg1	pork					1111:1114	shelf life of chilled pork	1089:1114	shelf life of chilled pork for 3 day	1089:1124	The sustained control LEO release from the pad can effectively inhibit the growth of E. coli during storage, thus prolonging shelf life of chilled pork for 3 day.
33545643	3	45	theme	water-absorbing	697:711	arg1	pad					713:715	KGM-g-PAA/PVA bacteriostatic water-absorbing pad	668:715	KGM-g-PAA/PVA bacteriostatic water-absorbing pad	668:715	KGM-g-PAA/PVA bacteriostatic water-absorbing pad was prepared via electrospinning technique, which can minimize the loss of LEO.
33545643	6	46	contain	have	1174:1177	arg1	pad					1166:1168	KGM-g-PAA/PVA pad	1152:1168	KGM-g-PAA/PVA pad	1152:1168	This study suggests that KGM-g-PAA/PVA pad may have a great potential in the field of intelligent packaging.
33545643	6	46	contain	have	1174:1177	arg2	potential					1187:1195	a great potential	1179:1195	a great potential	1179:1195	This study suggests that KGM-g-PAA/PVA pad may have a great potential in the field of intelligent packaging.
33545643	1	47	theme	food	329:332	arg1	materials					344:352	food packaging materials	329:352	food packaging materials	329:352	Lemon essential oils (LEO), as natural bacteriostatic agents, show significant loss in the preparation processes of food packaging materials, therefore, an effective encapsulation of LEO is urgent for realizing the protection.
33545643	1	47	theme	food	329:332	arg1	encapsulation					379:391	an effective encapsulation	366:391	an effective encapsulation of LEO	366:398	Lemon essential oils (LEO), as natural bacteriostatic agents, show significant loss in the preparation processes of food packaging materials, therefore, an effective encapsulation of LEO is urgent for realizing the protection.
33545643	1	48	dep	show	275:278	arg1	urgent					403:408	urgent	403:408	urgent	403:408	Lemon essential oils (LEO), as natural bacteriostatic agents, show significant loss in the preparation processes of food packaging materials, therefore, an effective encapsulation of LEO is urgent for realizing the protection.
33545643	0	49	dep	encapsulated	32:43	arg1	release					163:169	Sustained control release	145:169	Sustained control release	145:169	Lemon essential oil/vermiculite encapsulated in electrospun konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol bacteriostatic pad: Sustained control release and its application in food preservation.
33545643	0	49	dep	encapsulated	32:43	arg1	application					179:189	its application	175:189	its application in food preservation	175:210	Lemon essential oil/vermiculite encapsulated in electrospun konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol bacteriostatic pad: Sustained control release and its application in food preservation.
33545643	0	50	from	release	163:169	arg1	preservation					199:210	food preservation	194:210	food preservation	194:210	Lemon essential oil/vermiculite encapsulated in electrospun konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol bacteriostatic pad: Sustained control release and its application in food preservation.
33545643	5	51	theme	sustained	968:976	arg1	release					990:996	The sustained control LEO release	964:996	The sustained control LEO release from the pad	964:1009	The sustained control LEO release from the pad can effectively inhibit the growth of E. coli during storage, thus prolonging shelf life of chilled pork for 3 day.
33545643	0	52	theme	acrylic	93:99	arg1	acid					101:104	acrylic acid	93:104	acrylic acid	93:104	Lemon essential oil/vermiculite encapsulated in electrospun konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol bacteriostatic pad: Sustained control release and its application in food preservation.
33545643	0	52	theme	acrylic	93:99	arg1	glucomannan-grafted-poly					67:90	electrospun konjac glucomannan-grafted-poly	48:90	electrospun konjac glucomannan-grafted-poly (acrylic acid)	48:105	Lemon essential oil/vermiculite encapsulated in electrospun konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol bacteriostatic pad: Sustained control release and its application in food preservation.
33545643	5	53	theme	control	978:984	arg1	release					990:996	The sustained control LEO release	964:996	The sustained control LEO release from the pad	964:1009	The sustained control LEO release from the pad can effectively inhibit the growth of E. coli during storage, thus prolonging shelf life of chilled pork for 3 day.
33545643	3	54	theme	LEO	792:794	arg1	loss					784:787	the loss	780:787	the loss of LEO	780:794	KGM-g-PAA/PVA bacteriostatic water-absorbing pad was prepared via electrospinning technique, which can minimize the loss of LEO.
33545643	2	55	theme	stable	485:490	arg1	vermiculite					503:513	thermally stable and porous vermiculite	475:513	thermally stable and porous vermiculite (VML)	475:519	In this study, LEO was absorbed by thermally stable and porous vermiculite (VML) to form LEO/VML complex, which is further coupled with konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol (KGM-g-PAA/PVA) composite.
33545643	2	55	theme	stable	485:490	arg1	VML					516:518	VML	516:518	VML	516:518	In this study, LEO was absorbed by thermally stable and porous vermiculite (VML) to form LEO/VML complex, which is further coupled with konjac glucomannan-grafted-poly (acrylic acid)/polyvinyl alcohol (KGM-g-PAA/PVA) composite.
33545643	1	56	theme	packaging	334:342	arg1	materials					344:352	food packaging materials	329:352	food packaging materials	329:352	Lemon essential oils (LEO), as natural bacteriostatic agents, show significant loss in the preparation processes of food packaging materials, therefore, an effective encapsulation of LEO is urgent for realizing the protection.
33545643	1	56	theme	packaging	334:342	arg1	encapsulation					379:391	an effective encapsulation	366:391	an effective encapsulation of LEO	366:398	Lemon essential oils (LEO), as natural bacteriostatic agents, show significant loss in the preparation processes of food packaging materials, therefore, an effective encapsulation of LEO is urgent for realizing the protection.
33545643	5	57	theme	LEO	986:988	arg1	release					990:996	The sustained control LEO release	964:996	The sustained control LEO release from the pad	964:1009	The sustained control LEO release from the pad can effectively inhibit the growth of E. coli during storage, thus prolonging shelf life of chilled pork for 3 day.
33545643	6	58	theme	packaging	1225:1233	arg1	field					1204:1208	the field	1200:1208	the field of intelligent packaging	1200:1233	This study suggests that KGM-g-PAA/PVA pad may have a great potential in the field of intelligent packaging.
33545643	1	59	theme	natural	244:250	arg1	agents					267:272	natural bacteriostatic agents	244:272	natural bacteriostatic agents	244:272	Lemon essential oils (LEO), as natural bacteriostatic agents, show significant loss in the preparation processes of food packaging materials, therefore, an effective encapsulation of LEO is urgent for realizing the protection.
32919538	5	0	theme	nanostructure	714:726	arg1	viability					690:698	The cell viability	681:698	The cell viability of this novel nanostructure	681:726	The cell viability of this novel nanostructure was 89.2 % and its hemolytic effect was less than 6% while the highest concentration (1000 μg/mL) of this nanostructure was chosen for these purposes.
32919538	5	0	theme	nanostructure	714:726	arg1	%					737:737	89.2 %	732:737	89.2 %	732:737	The cell viability of this novel nanostructure was 89.2 % and its hemolytic effect was less than 6% while the highest concentration (1000 μg/mL) of this nanostructure was chosen for these purposes.
32919538	4	1	theme	biological	647:656	arg1	results					658:664	specific biological results	638:664	specific biological results	638:664	Because of using less toxic and high biocompatible materials, specific biological results were achieved.
32919538	2	2	dep	oxide	310:314	arg1	GO					317:318	GO	317:318	GO	317:318	In this study, graphene oxide (GO) was covalently modified by natural sodium alginate (Alg) polymer.
32919538	0	3	theme	blood	89:93	arg1	compatibility					95:107	improved blood compatibility	80:107	improved blood compatibility	80:107	Graphene oxide/alginate/silk fibroin composite as a novel bionanostructure with improved blood compatibility, less toxicity and enhanced mechanical properties.
32919538	6	4	dep	strength	938:945	arg1	0.87 ± 0.034					948:959	0.87 ± 0.034	948:959	0.87 ± 0.034 (MPa)	948:965	Also, high mechanical properties including the compressive strength (0.87 ± 0.034 (MPa)) and the compressive modulus (2.25 ± 0.091 (MPa)) were exposed.
32919538	6	4	dep	strength	938:945	arg1	MPa					962:964	MPa	962:964	MPa	962:964	Also, high mechanical properties including the compressive strength (0.87 ± 0.034 (MPa)) and the compressive modulus (2.25 ± 0.091 (MPa)) were exposed.
32919538	2	5	theme	sodium	356:361	arg1	alginate					363:370	natural sodium alginate	348:370	natural sodium alginate (Alg) polymer	348:384	In this study, graphene oxide (GO) was covalently modified by natural sodium alginate (Alg) polymer.
32919538	2	5	theme	sodium	356:361	arg1	Alg					373:375	Alg	373:375	Alg	373:375	In this study, graphene oxide (GO) was covalently modified by natural sodium alginate (Alg) polymer.
32919538	6	6	theme	compressive	926:936	arg1	strength					938:945	the compressive strength	922:945	the compressive strength (0.87 ± 0.034 (MPa))	922:966	Also, high mechanical properties including the compressive strength (0.87 ± 0.034 (MPa)) and the compressive modulus (2.25 ± 0.091 (MPa)) were exposed.
32919538	5	7	theme	cell	685:688	arg1	viability					690:698	The cell viability	681:698	The cell viability of this novel nanostructure	681:726	The cell viability of this novel nanostructure was 89.2 % and its hemolytic effect was less than 6% while the highest concentration (1000 μg/mL) of this nanostructure was chosen for these purposes.
32919538	5	7	theme	cell	685:688	arg1	%					737:737	89.2 %	732:737	89.2 %	732:737	The cell viability of this novel nanostructure was 89.2 % and its hemolytic effect was less than 6% while the highest concentration (1000 μg/mL) of this nanostructure was chosen for these purposes.
32919538	2	8	theme	graphene	301:308	arg1	oxide					310:314	graphene oxide	301:314	graphene oxide (GO)	301:319	In this study, graphene oxide (GO) was covalently modified by natural sodium alginate (Alg) polymer.
32919538	2	9	theme	alginate	363:370	arg1	polymer					378:384	natural sodium alginate (Alg) polymer	348:384	natural sodium alginate (Alg) polymer	348:384	In this study, graphene oxide (GO) was covalently modified by natural sodium alginate (Alg) polymer.
32919538	4	10	theme	specific	638:645	arg1	results					658:664	specific biological results	638:664	specific biological results	638:664	Because of using less toxic and high biocompatible materials, specific biological results were achieved.
32919538	5	11	theme	nanostructure	834:846	arg1	1000 μg/mL					814:823	1000 μg/mL	814:823	1000 μg/mL	814:823	The cell viability of this novel nanostructure was 89.2 % and its hemolytic effect was less than 6% while the highest concentration (1000 μg/mL) of this nanostructure was chosen for these purposes.
32919538	5	11	theme	nanostructure	834:846	arg1	concentration					799:811	the highest concentration	787:811	the highest concentration (1000 μg/mL) of this nanostructure	787:846	The cell viability of this novel nanostructure was 89.2 % and its hemolytic effect was less than 6% while the highest concentration (1000 μg/mL) of this nanostructure was chosen for these purposes.
32919538	3	12	theme	hybrid	440:445	arg1	GO/Alg/SF					465:473	GO/Alg/SF	465:473	GO/Alg/SF	465:473	By adding silk fibroin (SF) to this nanostructure, a hybrid nanobiocomposite (GO/Alg/SF) was resulted and its unique features were determined using FT-IR, EDX, FE-SEM, XRD and TG analyses.
32919538	3	12	theme	hybrid	440:445	arg1	nanobiocomposite					447:462	a hybrid nanobiocomposite	438:462	a hybrid nanobiocomposite (GO/Alg/SF)	438:474	By adding silk fibroin (SF) to this nanostructure, a hybrid nanobiocomposite (GO/Alg/SF) was resulted and its unique features were determined using FT-IR, EDX, FE-SEM, XRD and TG analyses.
32919538	3	13	theme	unique	497:502	arg1	features					504:511	its unique features	493:511	its unique features	493:511	By adding silk fibroin (SF) to this nanostructure, a hybrid nanobiocomposite (GO/Alg/SF) was resulted and its unique features were determined using FT-IR, EDX, FE-SEM, XRD and TG analyses.
32919538	3	14	theme	silk	397:400	arg1	SF					411:412	SF	411:412	SF	411:412	By adding silk fibroin (SF) to this nanostructure, a hybrid nanobiocomposite (GO/Alg/SF) was resulted and its unique features were determined using FT-IR, EDX, FE-SEM, XRD and TG analyses.
32919538	3	14	theme	silk	397:400	arg1	fibroin					402:408	silk fibroin	397:408	silk fibroin (SF)	397:413	By adding silk fibroin (SF) to this nanostructure, a hybrid nanobiocomposite (GO/Alg/SF) was resulted and its unique features were determined using FT-IR, EDX, FE-SEM, XRD and TG analyses.
32919538	4	15	theme	toxic	598:602	arg1	materials					627:635	less toxic and high biocompatible materials	593:635	less toxic and high biocompatible materials	593:635	Because of using less toxic and high biocompatible materials, specific biological results were achieved.
32919538	5	16	theme	hemolytic	747:755	arg1	effect					757:762	its hemolytic effect	743:762	its hemolytic effect	743:762	The cell viability of this novel nanostructure was 89.2 % and its hemolytic effect was less than 6% while the highest concentration (1000 μg/mL) of this nanostructure was chosen for these purposes.
32919538	7	17	theme	mentioned	1125:1133	arg1	properties					1135:1144	the mentioned properties	1121:1144	the mentioned properties	1121:1144	This nanostructure can be considered as a scaffold for wound dressing applications due to the mentioned properties.
32919538	6	18	dep	modulus	988:994	arg1	MPa					1011:1013	MPa	1011:1013	MPa	1011:1013	Also, high mechanical properties including the compressive strength (0.87 ± 0.034 (MPa)) and the compressive modulus (2.25 ± 0.091 (MPa)) were exposed.
32919538	6	18	dep	modulus	988:994	arg1	2.25 ± 0.091					997:1008	2.25 ± 0.091	997:1008	2.25 ± 0.091 (MPa)	997:1014	Also, high mechanical properties including the compressive strength (0.87 ± 0.034 (MPa)) and the compressive modulus (2.25 ± 0.091 (MPa)) were exposed.
32919538	4	19	theme	biocompatible	613:625	arg1	materials					627:635	less toxic and high biocompatible materials	593:635	less toxic and high biocompatible materials	593:635	Because of using less toxic and high biocompatible materials, specific biological results were achieved.
32919538	3	20	theme	EDX	542:544	arg1	analyses					566:573	FT-IR, EDX, FE-SEM, XRD and TG analyses	535:573	FT-IR, EDX, FE-SEM, XRD and TG analyses	535:573	By adding silk fibroin (SF) to this nanostructure, a hybrid nanobiocomposite (GO/Alg/SF) was resulted and its unique features were determined using FT-IR, EDX, FE-SEM, XRD and TG analyses.
32919538	4	21	theme	high	608:611	arg1	materials					627:635	less toxic and high biocompatible materials	593:635	less toxic and high biocompatible materials	593:635	Because of using less toxic and high biocompatible materials, specific biological results were achieved.
32919538	6	22	theme	mechanical	890:899	arg1	modulus					988:994	the compressive modulus	972:994	the compressive modulus (2.25 ± 0.091 (MPa))	972:1015	Also, high mechanical properties including the compressive strength (0.87 ± 0.034 (MPa)) and the compressive modulus (2.25 ± 0.091 (MPa)) were exposed.
32919538	6	22	theme	mechanical	890:899	arg1	strength					938:945	the compressive strength	922:945	the compressive strength (0.87 ± 0.034 (MPa))	922:966	Also, high mechanical properties including the compressive strength (0.87 ± 0.034 (MPa)) and the compressive modulus (2.25 ± 0.091 (MPa)) were exposed.
32919538	6	22	theme	mechanical	890:899	arg1	properties					901:910	high mechanical properties	885:910	high mechanical properties including the compressive strength (0.87 ± 0.034 (MPa)) and the compressive modulus (2.25 ± 0.091 (MPa))	885:1015	Also, high mechanical properties including the compressive strength (0.87 ± 0.034 (MPa)) and the compressive modulus (2.25 ± 0.091 (MPa)) were exposed.
32919538	0	23	theme	mechanical	137:146	arg1	properties					148:157	enhanced mechanical properties	128:157	enhanced mechanical properties	128:157	Graphene oxide/alginate/silk fibroin composite as a novel bionanostructure with improved blood compatibility, less toxicity and enhanced mechanical properties.
32919538	0	24	theme	fibroin	29:35	arg1	composite					37:45	fibroin composite	29:45	fibroin composite	29:45	Graphene oxide/alginate/silk fibroin composite as a novel bionanostructure with improved blood compatibility, less toxicity and enhanced mechanical properties.
32919538	0	24	theme	fibroin	29:35	arg1	bionanostructure					58:73	a novel bionanostructure	50:73	a novel bionanostructure with improved blood compatibility, less toxicity and enhanced mechanical properties	50:157	Graphene oxide/alginate/silk fibroin composite as a novel bionanostructure with improved blood compatibility, less toxicity and enhanced mechanical properties.
32919538	6	25	theme	high	885:888	arg1	modulus					988:994	the compressive modulus	972:994	the compressive modulus (2.25 ± 0.091 (MPa))	972:1015	Also, high mechanical properties including the compressive strength (0.87 ± 0.034 (MPa)) and the compressive modulus (2.25 ± 0.091 (MPa)) were exposed.
32919538	6	25	theme	high	885:888	arg1	strength					938:945	the compressive strength	922:945	the compressive strength (0.87 ± 0.034 (MPa))	922:966	Also, high mechanical properties including the compressive strength (0.87 ± 0.034 (MPa)) and the compressive modulus (2.25 ± 0.091 (MPa)) were exposed.
32919538	6	25	theme	high	885:888	arg1	properties					901:910	high mechanical properties	885:910	high mechanical properties including the compressive strength (0.87 ± 0.034 (MPa)) and the compressive modulus (2.25 ± 0.091 (MPa))	885:1015	Also, high mechanical properties including the compressive strength (0.87 ± 0.034 (MPa)) and the compressive modulus (2.25 ± 0.091 (MPa)) were exposed.
32919538	0	26	theme	enhanced	128:135	arg1	properties					148:157	enhanced mechanical properties	128:157	enhanced mechanical properties	128:157	Graphene oxide/alginate/silk fibroin composite as a novel bionanostructure with improved blood compatibility, less toxicity and enhanced mechanical properties.
32919538	3	27	theme	FT-IR	535:539	arg1	analyses					566:573	FT-IR, EDX, FE-SEM, XRD and TG analyses	535:573	FT-IR, EDX, FE-SEM, XRD and TG analyses	535:573	By adding silk fibroin (SF) to this nanostructure, a hybrid nanobiocomposite (GO/Alg/SF) was resulted and its unique features were determined using FT-IR, EDX, FE-SEM, XRD and TG analyses.
32919538	1	28	theme	biocompatible	217:229	arg1	nanostructures					231:244	biocompatible nanostructures	217:244	biocompatible nanostructures	217:244	For biomedical applications, the design and synthesis of biocompatible nanostructures, are considered as critical challenges.
32919538	6	29	theme	compressive	976:986	arg1	modulus					988:994	the compressive modulus	972:994	the compressive modulus (2.25 ± 0.091 (MPa))	972:1015	Also, high mechanical properties including the compressive strength (0.87 ± 0.034 (MPa)) and the compressive modulus (2.25 ± 0.091 (MPa)) were exposed.
32919538	1	30	theme	nanostructures	231:244	arg1	synthesis					204:212	synthesis	204:212	synthesis	204:212	For biomedical applications, the design and synthesis of biocompatible nanostructures, are considered as critical challenges.
32919538	1	30	theme	nanostructures	231:244	arg1	design					193:198	design	193:198	design	193:198	For biomedical applications, the design and synthesis of biocompatible nanostructures, are considered as critical challenges.
32919538	1	30	theme	nanostructures	231:244	arg1	challenges					274:283	critical challenges	265:283	critical challenges	265:283	For biomedical applications, the design and synthesis of biocompatible nanostructures, are considered as critical challenges.
32919538	3	31	theme	XRD	555:557	arg1	analyses					566:573	FT-IR, EDX, FE-SEM, XRD and TG analyses	535:573	FT-IR, EDX, FE-SEM, XRD and TG analyses	535:573	By adding silk fibroin (SF) to this nanostructure, a hybrid nanobiocomposite (GO/Alg/SF) was resulted and its unique features were determined using FT-IR, EDX, FE-SEM, XRD and TG analyses.
32919538	7	32	theme	dressing	1092:1099	arg1	applications					1101:1112	wound dressing applications	1086:1112	wound dressing applications due to the mentioned properties	1086:1144	This nanostructure can be considered as a scaffold for wound dressing applications due to the mentioned properties.
32919538	2	33	theme	natural	348:354	arg1	alginate					363:370	natural sodium alginate	348:370	natural sodium alginate (Alg) polymer	348:384	In this study, graphene oxide (GO) was covalently modified by natural sodium alginate (Alg) polymer.
32919538	2	33	theme	natural	348:354	arg1	Alg					373:375	Alg	373:375	Alg	373:375	In this study, graphene oxide (GO) was covalently modified by natural sodium alginate (Alg) polymer.
32919538	0	34	theme	novel	52:56	arg1	composite					37:45	fibroin composite	29:45	fibroin composite	29:45	Graphene oxide/alginate/silk fibroin composite as a novel bionanostructure with improved blood compatibility, less toxicity and enhanced mechanical properties.
32919538	0	34	theme	novel	52:56	arg1	bionanostructure					58:73	a novel bionanostructure	50:73	a novel bionanostructure with improved blood compatibility, less toxicity and enhanced mechanical properties	50:157	Graphene oxide/alginate/silk fibroin composite as a novel bionanostructure with improved blood compatibility, less toxicity and enhanced mechanical properties.
32919538	3	35	theme	FE-SEM	547:552	arg1	analyses					566:573	FT-IR, EDX, FE-SEM, XRD and TG analyses	535:573	FT-IR, EDX, FE-SEM, XRD and TG analyses	535:573	By adding silk fibroin (SF) to this nanostructure, a hybrid nanobiocomposite (GO/Alg/SF) was resulted and its unique features were determined using FT-IR, EDX, FE-SEM, XRD and TG analyses.
32919538	7	36	theme	due	1114:1116	arg1	applications					1101:1112	wound dressing applications	1086:1112	wound dressing applications due to the mentioned properties	1086:1144	This nanostructure can be considered as a scaffold for wound dressing applications due to the mentioned properties.
32919538	2	37	mod	modified	336:343	arg3	polymer					378:384	natural sodium alginate (Alg) polymer	348:384	natural sodium alginate (Alg) polymer	348:384	In this study, graphene oxide (GO) was covalently modified by natural sodium alginate (Alg) polymer.
32919538	2	37	mod	modified	336:343	arg1	oxide					310:314	graphene oxide	301:314	graphene oxide (GO)	301:319	In this study, graphene oxide (GO) was covalently modified by natural sodium alginate (Alg) polymer.
32919538	0	38	with	bionanostructure	58:73	arg1	properties					148:157	enhanced mechanical properties	128:157	enhanced mechanical properties	128:157	Graphene oxide/alginate/silk fibroin composite as a novel bionanostructure with improved blood compatibility, less toxicity and enhanced mechanical properties.
32919538	0	38	with	bionanostructure	58:73	arg1	compatibility					95:107	improved blood compatibility	80:107	improved blood compatibility	80:107	Graphene oxide/alginate/silk fibroin composite as a novel bionanostructure with improved blood compatibility, less toxicity and enhanced mechanical properties.
32919538	0	38	with	bionanostructure	58:73	arg1	toxicity					115:122	less toxicity	110:122	less toxicity	110:122	Graphene oxide/alginate/silk fibroin composite as a novel bionanostructure with improved blood compatibility, less toxicity and enhanced mechanical properties.
32919538	0	39	theme	improved	80:87	arg1	compatibility					95:107	improved blood compatibility	80:107	improved blood compatibility	80:107	Graphene oxide/alginate/silk fibroin composite as a novel bionanostructure with improved blood compatibility, less toxicity and enhanced mechanical properties.
32919538	5	40	theme	highest	791:797	arg1	1000 μg/mL					814:823	1000 μg/mL	814:823	1000 μg/mL	814:823	The cell viability of this novel nanostructure was 89.2 % and its hemolytic effect was less than 6% while the highest concentration (1000 μg/mL) of this nanostructure was chosen for these purposes.
32919538	5	40	theme	highest	791:797	arg1	concentration					799:811	the highest concentration	787:811	the highest concentration (1000 μg/mL) of this nanostructure	787:846	The cell viability of this novel nanostructure was 89.2 % and its hemolytic effect was less than 6% while the highest concentration (1000 μg/mL) of this nanostructure was chosen for these purposes.
32919538	5	41	theme	novel	708:712	arg1	nanostructure					714:726	this novel nanostructure	703:726	this novel nanostructure	703:726	The cell viability of this novel nanostructure was 89.2 % and its hemolytic effect was less than 6% while the highest concentration (1000 μg/mL) of this nanostructure was chosen for these purposes.
32919538	7	42	theme	wound	1086:1090	arg1	applications					1101:1112	wound dressing applications	1086:1112	wound dressing applications due to the mentioned properties	1086:1144	This nanostructure can be considered as a scaffold for wound dressing applications due to the mentioned properties.
32919538	3	43	theme	TG	563:564	arg1	analyses					566:573	FT-IR, EDX, FE-SEM, XRD and TG analyses	535:573	FT-IR, EDX, FE-SEM, XRD and TG analyses	535:573	By adding silk fibroin (SF) to this nanostructure, a hybrid nanobiocomposite (GO/Alg/SF) was resulted and its unique features were determined using FT-IR, EDX, FE-SEM, XRD and TG analyses.
32919538	1	44	theme	biomedical	164:173	arg1	applications					175:186	biomedical applications	164:186	biomedical applications	164:186	For biomedical applications, the design and synthesis of biocompatible nanostructures, are considered as critical challenges.
32919538	1	45	theme	critical	265:272	arg1	synthesis					204:212	synthesis	204:212	synthesis	204:212	For biomedical applications, the design and synthesis of biocompatible nanostructures, are considered as critical challenges.
32919538	1	45	theme	critical	265:272	arg1	design					193:198	design	193:198	design	193:198	For biomedical applications, the design and synthesis of biocompatible nanostructures, are considered as critical challenges.
32919538	1	45	theme	critical	265:272	arg1	challenges					274:283	critical challenges	265:283	critical challenges	265:283	For biomedical applications, the design and synthesis of biocompatible nanostructures, are considered as critical challenges.
33464809	7	0	theme	disrupted	1523:1531	arg1	membrane					1533:1540	a disrupted membrane	1521:1540	a disrupted membrane	1521:1540	The changes in the bacterial morphology with a disrupted membrane were further confirmed through various imaging techniques like confocal laser scanning microscopy, FESEM, AFM, and 3D digital microscopy.
33464809	4	1	theme	pristine	877:884	arg1	architecture					890:901	pristine MFC architecture	877:901	pristine MFC architecture	877:901	At an optimal concentration of 0.3 wt % cCNCs (i.e., for ciMFC/cCNCs_0.3), an improvement of around 106% in compressive strength and 175% increment in modulus were achieved as compared to pristine MFC architecture.
33464809	10	2	theme	natural	2143:2149	arg1	extracts					2151:2158	crude natural extracts	2137:2158	crude natural extracts	2137:2158	Hence, this work provides avenues for the application of crude natural extracts in developing novel forms of advanced functional biomaterials that can be used for assorted biological/healthcare applications such as wound care and antimicrobial filtering units.
33464809	6	3	dep	concentration	1343:1355	arg1	mg/mL					1370:1374	here 1 mg/mL	1363:1374	here 1 mg/mL	1363:1374	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	6	3	dep	concentration	1343:1355	arg1	MIC					1358:1360	MIC	1358:1360	MIC	1358:1360	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	6	4	theme	inhibitory	1332:1341	arg1	concentration					1343:1355	minimum inhibitory concentration	1324:1355	minimum inhibitory concentration (MIC, here 1 mg/mL)	1324:1375	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	3	5	theme	lightweight	589:599	arg1	architectures					632:644	lightweight and flexible composite aerogel architectures	589:644	lightweight and flexible composite aerogel architectures impregnated with ciE, namely, ciMFC/cCNCs	589:686	To this MFC suspension, carboxylated cellulose nanocrystals (cCNCs) were homogeneously mixed and the viscous gel thus obtained was freeze-dried to obtain lightweight and flexible composite aerogel architectures impregnated with ciE, namely, ciMFC/cCNCs.
33464809	7	6	theme	bacterial	1495:1503	arg1	morphology					1505:1514	the bacterial morphology	1491:1514	the bacterial morphology with a disrupted membrane	1491:1540	The changes in the bacterial morphology with a disrupted membrane were further confirmed through various imaging techniques like confocal laser scanning microscopy, FESEM, AFM, and 3D digital microscopy.
33464809	6	7	theme	antibacterial	1231:1243	arg1	assays					1245:1250	various antibacterial assays	1223:1250	various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests	1223:1473	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	10	8	theme	novel	2174:2178	arg1	forms					2180:2184	novel forms	2174:2184	novel forms of advanced functional biomaterials that can be used for assorted biological/healthcare applications such as wound care and antimicrobial filtering units	2174:2338	Hence, this work provides avenues for the application of crude natural extracts in developing novel forms of advanced functional biomaterials that can be used for assorted biological/healthcare applications such as wound care and antimicrobial filtering units.
33464809	2	9	dep	In	313:314	arg1	situ					316:319	situ	316:319	situ	316:319	In situ fibrillation of purified cellulose was done in cinnamon extract (ciE) to obtain microfibrillated cellulose (MFC).
33464809	1	10	theme	product	210:216	arg1	potential					178:186	the potential	174:186	the potential of a herbal medicinal product as a natural bioactive ingredient to generate nanocellulose-based antibacterial architectures	174:310	This work is strategically premeditated to study the potential of a herbal medicinal product as a natural bioactive ingredient to generate nanocellulose-based antibacterial architectures.
33464809	7	11	from	changes	1480:1486	arg1	morphology					1505:1514	the bacterial morphology	1491:1514	the bacterial morphology with a disrupted membrane	1491:1540	The changes in the bacterial morphology with a disrupted membrane were further confirmed through various imaging techniques like confocal laser scanning microscopy, FESEM, AFM, and 3D digital microscopy.
33464809	4	12	theme	wt	724:725	arg1	cCNCs					729:733	0.3 wt % cCNCs	720:733	0.3 wt % cCNCs (i.e., for ciMFC/cCNCs_0.3)	720:761	At an optimal concentration of 0.3 wt % cCNCs (i.e., for ciMFC/cCNCs_0.3), an improvement of around 106% in compressive strength and 175% increment in modulus were achieved as compared to pristine MFC architecture.
33464809	8	13	theme	novel	1927:1931	arg1	biomaterial					1933:1943	the novel biomaterial	1923:1943	the novel biomaterial	1923:1943	The dry composite architecture showed the persuasive capability of suppressing the growth of airborne bacteria, which in combination with antibacterial efficiency in the wet state is considered as an imperative aspect for a material to act as the novel biomaterial.
33464809	2	14	theme	cinnamon	368:375	arg1	ciE					386:388	ciE	386:388	ciE	386:388	In situ fibrillation of purified cellulose was done in cinnamon extract (ciE) to obtain microfibrillated cellulose (MFC).
33464809	2	14	theme	cinnamon	368:375	arg1	extract					377:383	cinnamon extract	368:383	cinnamon extract (ciE)	368:389	In situ fibrillation of purified cellulose was done in cinnamon extract (ciE) to obtain microfibrillated cellulose (MFC).
33464809	8	15	theme	bacteria	1782:1789	arg1	growth					1763:1768	the growth	1759:1768	the growth of airborne bacteria, which in combination with antibacterial efficiency in the wet state is considered as an imperative aspect for a material to act as the novel biomaterial	1759:1943	The dry composite architecture showed the persuasive capability of suppressing the growth of airborne bacteria, which in combination with antibacterial efficiency in the wet state is considered as an imperative aspect for a material to act as the novel biomaterial.
33464809	10	16	theme	assorted	2243:2250	arg1	care					2301:2304	wound care	2295:2304	wound care	2295:2304	Hence, this work provides avenues for the application of crude natural extracts in developing novel forms of advanced functional biomaterials that can be used for assorted biological/healthcare applications such as wound care and antimicrobial filtering units.
33464809	10	16	theme	assorted	2243:2250	arg1	units					2334:2338	antimicrobial filtering units	2310:2338	antimicrobial filtering units	2310:2338	Hence, this work provides avenues for the application of crude natural extracts in developing novel forms of advanced functional biomaterials that can be used for assorted biological/healthcare applications such as wound care and antimicrobial filtering units.
33464809	10	16	theme	assorted	2243:2250	arg1	applications					2274:2285	assorted biological/healthcare applications	2243:2285	assorted biological/healthcare applications such as wound care and antimicrobial filtering units	2243:2338	Hence, this work provides avenues for the application of crude natural extracts in developing novel forms of advanced functional biomaterials that can be used for assorted biological/healthcare applications such as wound care and antimicrobial filtering units.
33464809	10	17	theme	functional	2198:2207	arg1	biomaterials					2209:2220	advanced functional biomaterials	2189:2220	advanced functional biomaterials that can be used for assorted biological/healthcare applications such as wound care and antimicrobial filtering units	2189:2338	Hence, this work provides avenues for the application of crude natural extracts in developing novel forms of advanced functional biomaterials that can be used for assorted biological/healthcare applications such as wound care and antimicrobial filtering units.
33464809	6	18	theme	minimum	1378:1384	arg1	concentration					1399:1411	minimum bactericidal concentration	1378:1411	minimum bactericidal concentration (MBC, here 3-5 mg/mL)	1378:1433	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	3	19	theme	flexible	605:612	arg1	architectures					632:644	lightweight and flexible composite aerogel architectures	589:644	lightweight and flexible composite aerogel architectures impregnated with ciE, namely, ciMFC/cCNCs	589:686	To this MFC suspension, carboxylated cellulose nanocrystals (cCNCs) were homogeneously mixed and the viscous gel thus obtained was freeze-dried to obtain lightweight and flexible composite aerogel architectures impregnated with ciE, namely, ciMFC/cCNCs.
33464809	1	20	theme	bioactive	231:239	arg1	ingredient					241:250	a natural bioactive ingredient	221:250	a natural bioactive ingredient to generate nanocellulose-based antibacterial architectures	221:310	This work is strategically premeditated to study the potential of a herbal medicinal product as a natural bioactive ingredient to generate nanocellulose-based antibacterial architectures.
33464809	9	21	theme	antibacterial	2002:2014	arg1	performance					2016:2026	excellent antibacterial performance	1992:2026	excellent antibacterial performance under real "in use" contamination prone conditions	1992:2077	Furthermore, these architectures demonstrated excellent antibacterial performance under real "in use" contamination prone conditions.
33464809	4	22	theme	optimal	695:701	arg1	concentration					703:715	an optimal concentration	692:715	an optimal concentration of 0.3 wt % cCNCs (i.e., for ciMFC/cCNCs_0.3)	692:761	At an optimal concentration of 0.3 wt % cCNCs (i.e., for ciMFC/cCNCs_0.3), an improvement of around 106% in compressive strength and 175% increment in modulus were achieved as compared to pristine MFC architecture.
33464809	7	23	theme	3D	1657:1658	arg1	microscopy					1668:1677	3D digital microscopy	1657:1677	3D digital microscopy	1657:1677	The changes in the bacterial morphology with a disrupted membrane were further confirmed through various imaging techniques like confocal laser scanning microscopy, FESEM, AFM, and 3D digital microscopy.
33464809	6	24	theme	bacterial	1285:1293	arg1	growth					1295:1300	bacterial growth	1285:1300	bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests	1285:1473	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	5	25	theme	dense	1070:1074	arg1	microstructures					1088:1102	dense and uniform microstructures	1070:1102	dense and uniform microstructures	1070:1102	The efficient loading and interaction of ciE components, specifically cinnamaldehyde, with MFC and cCNCs resulted in developing competent antibacterial surfaces with dense and uniform microstructures.
33464809	4	26	from	increment	827:835	arg1	strength					809:816	compressive strength	797:816	compressive strength	797:816	At an optimal concentration of 0.3 wt % cCNCs (i.e., for ciMFC/cCNCs_0.3), an improvement of around 106% in compressive strength and 175% increment in modulus were achieved as compared to pristine MFC architecture.
33464809	4	26	from	increment	827:835	arg1	modulus					840:846	modulus	840:846	modulus	840:846	At an optimal concentration of 0.3 wt % cCNCs (i.e., for ciMFC/cCNCs_0.3), an improvement of around 106% in compressive strength and 175% increment in modulus were achieved as compared to pristine MFC architecture.
33464809	6	27	from	BacLight	1450:1457	arg1	method					1277:1282	the zone inhibition method	1257:1282	the zone inhibition method	1257:1282	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	6	27	from	BacLight	1450:1457	arg1	observation					1302:1312	bacterial growth observation	1285:1312	bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests	1285:1473	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	5	28	theme	ciE	945:947	arg1	components					949:958	ciE components	945:958	ciE components	945:958	The efficient loading and interaction of ciE components, specifically cinnamaldehyde, with MFC and cCNCs resulted in developing competent antibacterial surfaces with dense and uniform microstructures.
33464809	5	28	theme	ciE	945:947	arg1	cinnamaldehyde					974:987	cinnamaldehyde	974:987	specifically cinnamaldehyde	961:987	The efficient loading and interaction of ciE components, specifically cinnamaldehyde, with MFC and cCNCs resulted in developing competent antibacterial surfaces with dense and uniform microstructures.
33464809	6	29	theme	zone	1261:1264	arg1	method					1277:1282	the zone inhibition method	1257:1282	the zone inhibition method	1257:1282	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	6	29	theme	zone	1261:1264	arg1	observation					1302:1312	bacterial growth observation	1285:1312	bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests	1285:1473	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	9	30	theme	use	2043:2045	arg1	conditions					2068:2077	real "in use" contamination prone conditions	2034:2077	real "in use" contamination prone conditions	2034:2077	Furthermore, these architectures demonstrated excellent antibacterial performance under real "in use" contamination prone conditions.
33464809	4	31	theme	cCNCs	729:733	arg1	concentration					703:715	an optimal concentration	692:715	an optimal concentration of 0.3 wt % cCNCs (i.e., for ciMFC/cCNCs_0.3)	692:761	At an optimal concentration of 0.3 wt % cCNCs (i.e., for ciMFC/cCNCs_0.3), an improvement of around 106% in compressive strength and 175% increment in modulus were achieved as compared to pristine MFC architecture.
33464809	0	32	theme	Bioactive	96:104	arg1	cassia					117:122	Bioactive Cinnamomum cassia	96:122	Bioactive Cinnamomum cassia	96:122	Microdesigned Nanocellulose-Based Flexible Antibacterial Aerogel Architectures Impregnated with Bioactive Cinnamomum cassia.
33464809	0	33	theme	Flexible	34:41	arg1	Architectures					65:77	Microdesigned Nanocellulose-Based Flexible Antibacterial Aerogel Architectures	0:77	Microdesigned Nanocellulose-Based Flexible Antibacterial Aerogel Architectures	0:77	Microdesigned Nanocellulose-Based Flexible Antibacterial Aerogel Architectures Impregnated with Bioactive Cinnamomum cassia.
33464809	8	34	theme	wet	1850:1852	arg1	state					1854:1858	the wet state	1846:1858	the wet state	1846:1858	The dry composite architecture showed the persuasive capability of suppressing the growth of airborne bacteria, which in combination with antibacterial efficiency in the wet state is considered as an imperative aspect for a material to act as the novel biomaterial.
33464809	9	35	theme	contamination	2048:2060	arg1	conditions					2068:2077	real "in use" contamination prone conditions	2034:2077	real "in use" contamination prone conditions	2034:2077	Furthermore, these architectures demonstrated excellent antibacterial performance under real "in use" contamination prone conditions.
33464809	6	36	dep	concentration	1399:1411	arg1	MBC					1414:1416	MBC	1414:1416	MBC	1414:1416	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	6	36	dep	concentration	1399:1411	arg1	mg/mL					1428:1432	here 3-5 mg/mL	1419:1432	here 3-5 mg/mL	1419:1432	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	1	37	theme	herbal	193:198	arg1	product					210:216	a herbal medicinal product	191:216	a herbal medicinal product	191:216	This work is strategically premeditated to study the potential of a herbal medicinal product as a natural bioactive ingredient to generate nanocellulose-based antibacterial architectures.
33464809	7	38	theme	scanning	1620:1627	arg1	microscopy					1629:1638	confocal laser scanning microscopy	1605:1638	confocal laser scanning microscopy	1605:1638	The changes in the bacterial morphology with a disrupted membrane were further confirmed through various imaging techniques like confocal laser scanning microscopy, FESEM, AFM, and 3D digital microscopy.
33464809	10	39	theme	wound	2295:2299	arg1	care					2301:2304	wound care	2295:2304	wound care	2295:2304	Hence, this work provides avenues for the application of crude natural extracts in developing novel forms of advanced functional biomaterials that can be used for assorted biological/healthcare applications such as wound care and antimicrobial filtering units.
33464809	10	40	used	used	2234:2237	arg2	biomaterials					2209:2220	advanced functional biomaterials	2189:2220	advanced functional biomaterials that can be used for assorted biological/healthcare applications such as wound care and antimicrobial filtering units	2189:2338	Hence, this work provides avenues for the application of crude natural extracts in developing novel forms of advanced functional biomaterials that can be used for assorted biological/healthcare applications such as wound care and antimicrobial filtering units.
33464809	2	41	theme	cellulose	346:354	arg1	fibrillation					321:332	In situ fibrillation	313:332	In situ fibrillation of purified cellulose	313:354	In situ fibrillation of purified cellulose was done in cinnamon extract (ciE) to obtain microfibrillated cellulose (MFC).
33464809	7	42	theme	confocal	1605:1612	arg1	microscopy					1629:1638	confocal laser scanning microscopy	1605:1638	confocal laser scanning microscopy	1605:1638	The changes in the bacterial morphology with a disrupted membrane were further confirmed through various imaging techniques like confocal laser scanning microscopy, FESEM, AFM, and 3D digital microscopy.
33464809	8	43	theme	imperative	1880:1889	arg1	bacteria					1782:1789	airborne bacteria	1773:1789	airborne bacteria	1773:1789	The dry composite architecture showed the persuasive capability of suppressing the growth of airborne bacteria, which in combination with antibacterial efficiency in the wet state is considered as an imperative aspect for a material to act as the novel biomaterial.
33464809	8	43	theme	imperative	1880:1889	arg1	aspect					1891:1896	an imperative aspect	1877:1896	an imperative aspect for a material to act as the novel biomaterial	1877:1943	The dry composite architecture showed the persuasive capability of suppressing the growth of airborne bacteria, which in combination with antibacterial efficiency in the wet state is considered as an imperative aspect for a material to act as the novel biomaterial.
33464809	10	44	theme	filtering	2324:2332	arg1	units					2334:2338	antimicrobial filtering units	2310:2338	antimicrobial filtering units	2310:2338	Hence, this work provides avenues for the application of crude natural extracts in developing novel forms of advanced functional biomaterials that can be used for assorted biological/healthcare applications such as wound care and antimicrobial filtering units.
33464809	3	45	theme	carboxylated	459:470	arg1	nanocrystals					482:493	carboxylated cellulose nanocrystals	459:493	carboxylated cellulose nanocrystals (cCNCs)	459:501	To this MFC suspension, carboxylated cellulose nanocrystals (cCNCs) were homogeneously mixed and the viscous gel thus obtained was freeze-dried to obtain lightweight and flexible composite aerogel architectures impregnated with ciE, namely, ciMFC/cCNCs.
33464809	3	45	theme	carboxylated	459:470	arg1	cCNCs					496:500	cCNCs	496:500	cCNCs	496:500	To this MFC suspension, carboxylated cellulose nanocrystals (cCNCs) were homogeneously mixed and the viscous gel thus obtained was freeze-dried to obtain lightweight and flexible composite aerogel architectures impregnated with ciE, namely, ciMFC/cCNCs.
33464809	0	46	theme	Microdesigned	0:12	arg1	Architectures					65:77	Microdesigned Nanocellulose-Based Flexible Antibacterial Aerogel Architectures	0:77	Microdesigned Nanocellulose-Based Flexible Antibacterial Aerogel Architectures	0:77	Microdesigned Nanocellulose-Based Flexible Antibacterial Aerogel Architectures Impregnated with Bioactive Cinnamomum cassia.
33464809	6	47	theme	viability	1459:1467	arg1	tests					1469:1473	viability tests	1459:1473	viability tests	1459:1473	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	7	48	theme	imaging	1581:1587	arg1	techniques					1589:1598	various imaging techniques	1573:1598	various imaging techniques like confocal laser scanning microscopy, FESEM, AFM, and 3D digital microscopy	1573:1677	The changes in the bacterial morphology with a disrupted membrane were further confirmed through various imaging techniques like confocal laser scanning microscopy, FESEM, AFM, and 3D digital microscopy.
33464809	6	49	theme	Live/Dead	1440:1448	arg1	BacLight					1450:1457	Live/Dead BacLight	1440:1457	Live/Dead BacLight	1440:1457	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	0	50	theme	Aerogel	57:63	arg1	Architectures					65:77	Microdesigned Nanocellulose-Based Flexible Antibacterial Aerogel Architectures	0:77	Microdesigned Nanocellulose-Based Flexible Antibacterial Aerogel Architectures	0:77	Microdesigned Nanocellulose-Based Flexible Antibacterial Aerogel Architectures Impregnated with Bioactive Cinnamomum cassia.
33464809	5	51	theme	antibacterial	1042:1054	arg1	surfaces					1056:1063	competent antibacterial surfaces	1032:1063	competent antibacterial surfaces	1032:1063	The efficient loading and interaction of ciE components, specifically cinnamaldehyde, with MFC and cCNCs resulted in developing competent antibacterial surfaces with dense and uniform microstructures.
33464809	8	52	theme	dry	1684:1686	arg1	architecture					1698:1709	The dry composite architecture	1680:1709	The dry composite architecture	1680:1709	The dry composite architecture showed the persuasive capability of suppressing the growth of airborne bacteria, which in combination with antibacterial efficiency in the wet state is considered as an imperative aspect for a material to act as the novel biomaterial.
33464809	6	53	from	concentration	1343:1355	arg1	method					1277:1282	the zone inhibition method	1257:1282	the zone inhibition method	1257:1282	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	6	53	from	concentration	1343:1355	arg1	observation					1302:1312	bacterial growth observation	1285:1312	bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests	1285:1473	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	6	54	theme	optimized	1159:1167	arg1	architectures					1169:1181	the optimized architectures	1155:1181	the optimized architectures	1155:1181	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	4	55	theme	175	822:824	arg1	%					825:825	%	825:825	%	825:825	At an optimal concentration of 0.3 wt % cCNCs (i.e., for ciMFC/cCNCs_0.3), an improvement of around 106% in compressive strength and 175% increment in modulus were achieved as compared to pristine MFC architecture.
33464809	1	56	theme	antibacterial	284:296	arg1	architectures					298:310	nanocellulose-based antibacterial architectures	264:310	nanocellulose-based antibacterial architectures	264:310	This work is strategically premeditated to study the potential of a herbal medicinal product as a natural bioactive ingredient to generate nanocellulose-based antibacterial architectures.
33464809	10	57	theme	extracts	2151:2158	arg1	application					2122:2132	the application	2118:2132	the application of crude natural extracts in developing novel forms of advanced functional biomaterials that can be used for assorted biological/healthcare applications such as wound care and antimicrobial filtering units	2118:2338	Hence, this work provides avenues for the application of crude natural extracts in developing novel forms of advanced functional biomaterials that can be used for assorted biological/healthcare applications such as wound care and antimicrobial filtering units.
33464809	2	58	theme	microfibrillated	401:416	arg1	MFC					429:431	MFC	429:431	MFC	429:431	In situ fibrillation of purified cellulose was done in cinnamon extract (ciE) to obtain microfibrillated cellulose (MFC).
33464809	2	58	theme	microfibrillated	401:416	arg1	cellulose					418:426	microfibrillated cellulose	401:426	microfibrillated cellulose (MFC)	401:432	In situ fibrillation of purified cellulose was done in cinnamon extract (ciE) to obtain microfibrillated cellulose (MFC).
33464809	6	59	from	OD600	1317:1321	arg1	method					1277:1282	the zone inhibition method	1257:1282	the zone inhibition method	1257:1282	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	6	59	from	OD600	1317:1321	arg1	observation					1302:1312	bacterial growth observation	1285:1312	bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests	1285:1473	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	6	60	theme	antimicrobial	1129:1141	arg1	activity					1143:1150	Excellent and long-term antimicrobial activity	1105:1150	activity	1143:1150	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	10	61	theme	crude	2137:2141	arg1	extracts					2151:2158	crude natural extracts	2137:2158	crude natural extracts	2137:2158	Hence, this work provides avenues for the application of crude natural extracts in developing novel forms of advanced functional biomaterials that can be used for assorted biological/healthcare applications such as wound care and antimicrobial filtering units.
33464809	4	62	theme	MFC	886:888	arg1	architecture					890:901	pristine MFC architecture	877:901	pristine MFC architecture	877:901	At an optimal concentration of 0.3 wt % cCNCs (i.e., for ciMFC/cCNCs_0.3), an improvement of around 106% in compressive strength and 175% increment in modulus were achieved as compared to pristine MFC architecture.
33464809	8	63	theme	antibacterial	1818:1830	arg1	efficiency					1832:1841	antibacterial efficiency	1818:1841	antibacterial efficiency in the wet state	1818:1858	The dry composite architecture showed the persuasive capability of suppressing the growth of airborne bacteria, which in combination with antibacterial efficiency in the wet state is considered as an imperative aspect for a material to act as the novel biomaterial.
33464809	6	64	theme	minimum	1324:1330	arg1	concentration					1343:1355	minimum inhibitory concentration	1324:1355	minimum inhibitory concentration (MIC, here 1 mg/mL)	1324:1375	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	2	65	theme	purified	337:344	arg1	cellulose					346:354	purified cellulose	337:354	purified cellulose	337:354	In situ fibrillation of purified cellulose was done in cinnamon extract (ciE) to obtain microfibrillated cellulose (MFC).
33464809	1	66	theme	medicinal	200:208	arg1	product					210:216	a herbal medicinal product	191:216	a herbal medicinal product	191:216	This work is strategically premeditated to study the potential of a herbal medicinal product as a natural bioactive ingredient to generate nanocellulose-based antibacterial architectures.
33464809	6	67	theme	various	1223:1229	arg1	assays					1245:1250	various antibacterial assays	1223:1250	various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests	1223:1473	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	3	68	theme	composite	614:622	arg1	architectures					632:644	lightweight and flexible composite aerogel architectures	589:644	lightweight and flexible composite aerogel architectures impregnated with ciE, namely, ciMFC/cCNCs	589:686	To this MFC suspension, carboxylated cellulose nanocrystals (cCNCs) were homogeneously mixed and the viscous gel thus obtained was freeze-dried to obtain lightweight and flexible composite aerogel architectures impregnated with ciE, namely, ciMFC/cCNCs.
33464809	10	69	theme	biomaterials	2209:2220	arg1	forms					2180:2184	novel forms	2174:2184	novel forms of advanced functional biomaterials that can be used for assorted biological/healthcare applications such as wound care and antimicrobial filtering units	2174:2338	Hence, this work provides avenues for the application of crude natural extracts in developing novel forms of advanced functional biomaterials that can be used for assorted biological/healthcare applications such as wound care and antimicrobial filtering units.
33464809	8	70	with	combination	1801:1811	arg1	efficiency					1832:1841	antibacterial efficiency	1818:1841	antibacterial efficiency in the wet state	1818:1858	The dry composite architecture showed the persuasive capability of suppressing the growth of airborne bacteria, which in combination with antibacterial efficiency in the wet state is considered as an imperative aspect for a material to act as the novel biomaterial.
33464809	6	71	theme	bactericidal	1386:1397	arg1	concentration					1399:1411	minimum bactericidal concentration	1378:1411	minimum bactericidal concentration (MBC, here 3-5 mg/mL)	1378:1433	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	8	72	theme	airborne	1773:1780	arg1	bacteria					1782:1789	airborne bacteria	1773:1789	airborne bacteria	1773:1789	The dry composite architecture showed the persuasive capability of suppressing the growth of airborne bacteria, which in combination with antibacterial efficiency in the wet state is considered as an imperative aspect for a material to act as the novel biomaterial.
33464809	8	72	theme	airborne	1773:1780	arg1	aspect					1891:1896	an imperative aspect	1877:1896	an imperative aspect for a material to act as the novel biomaterial	1877:1943	The dry composite architecture showed the persuasive capability of suppressing the growth of airborne bacteria, which in combination with antibacterial efficiency in the wet state is considered as an imperative aspect for a material to act as the novel biomaterial.
33464809	1	73	theme	natural	223:229	arg1	ingredient					241:250	a natural bioactive ingredient	221:250	a natural bioactive ingredient to generate nanocellulose-based antibacterial architectures	221:310	This work is strategically premeditated to study the potential of a herbal medicinal product as a natural bioactive ingredient to generate nanocellulose-based antibacterial architectures.
33464809	3	74	theme	aerogel	624:630	arg1	architectures					632:644	lightweight and flexible composite aerogel architectures	589:644	lightweight and flexible composite aerogel architectures impregnated with ciE, namely, ciMFC/cCNCs	589:686	To this MFC suspension, carboxylated cellulose nanocrystals (cCNCs) were homogeneously mixed and the viscous gel thus obtained was freeze-dried to obtain lightweight and flexible composite aerogel architectures impregnated with ciE, namely, ciMFC/cCNCs.
33464809	7	75	theme	digital	1660:1666	arg1	microscopy					1668:1677	3D digital microscopy	1657:1677	3D digital microscopy	1657:1677	The changes in the bacterial morphology with a disrupted membrane were further confirmed through various imaging techniques like confocal laser scanning microscopy, FESEM, AFM, and 3D digital microscopy.
33464809	9	76	theme	excellent	1992:2000	arg1	performance					2016:2026	excellent antibacterial performance	1992:2026	excellent antibacterial performance under real "in use" contamination prone conditions	1992:2077	Furthermore, these architectures demonstrated excellent antibacterial performance under real "in use" contamination prone conditions.
33464809	6	77	theme	growth	1295:1300	arg1	method					1277:1282	the zone inhibition method	1257:1282	the zone inhibition method	1257:1282	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	6	77	theme	growth	1295:1300	arg1	observation					1302:1312	bacterial growth observation	1285:1312	bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests	1285:1473	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	8	78	from	efficiency	1832:1841	arg1	state					1854:1858	the wet state	1846:1858	the wet state	1846:1858	The dry composite architecture showed the persuasive capability of suppressing the growth of airborne bacteria, which in combination with antibacterial efficiency in the wet state is considered as an imperative aspect for a material to act as the novel biomaterial.
33464809	9	79	theme	real	2034:2037	arg1	conditions					2068:2077	real "in use" contamination prone conditions	2034:2077	real "in use" contamination prone conditions	2034:2077	Furthermore, these architectures demonstrated excellent antibacterial performance under real "in use" contamination prone conditions.
33464809	6	80	theme	inhibition	1266:1275	arg1	method					1277:1282	the zone inhibition method	1257:1282	the zone inhibition method	1257:1282	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	6	80	theme	inhibition	1266:1275	arg1	observation					1302:1312	bacterial growth observation	1285:1312	bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests	1285:1473	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	9	81	theme	in	2040:2041	arg1	conditions					2068:2077	real "in use" contamination prone conditions	2034:2077	real "in use" contamination prone conditions	2034:2077	Furthermore, these architectures demonstrated excellent antibacterial performance under real "in use" contamination prone conditions.
33464809	0	82	theme	Cinnamomum	106:115	arg1	cassia					117:122	Bioactive Cinnamomum cassia	96:122	Bioactive Cinnamomum cassia	96:122	Microdesigned Nanocellulose-Based Flexible Antibacterial Aerogel Architectures Impregnated with Bioactive Cinnamomum cassia.
33464809	2	83	theme	In	313:314	arg1	fibrillation					321:332	In situ fibrillation	313:332	In situ fibrillation of purified cellulose	313:354	In situ fibrillation of purified cellulose was done in cinnamon extract (ciE) to obtain microfibrillated cellulose (MFC).
33464809	9	84	theme	"	2046:2046	arg1	conditions					2068:2077	real "in use" contamination prone conditions	2034:2077	real "in use" contamination prone conditions	2034:2077	Furthermore, these architectures demonstrated excellent antibacterial performance under real "in use" contamination prone conditions.
33464809	4	85	theme	%	727:727	arg1	cCNCs					729:733	0.3 wt % cCNCs	720:733	0.3 wt % cCNCs (i.e., for ciMFC/cCNCs_0.3)	720:761	At an optimal concentration of 0.3 wt % cCNCs (i.e., for ciMFC/cCNCs_0.3), an improvement of around 106% in compressive strength and 175% increment in modulus were achieved as compared to pristine MFC architecture.
33464809	5	86	theme	efficient	908:916	arg1	loading					918:924	The efficient loading	904:924	The efficient loading	904:924	The efficient loading and interaction of ciE components, specifically cinnamaldehyde, with MFC and cCNCs resulted in developing competent antibacterial surfaces with dense and uniform microstructures.
33464809	7	87	theme	laser	1614:1618	arg1	microscopy					1629:1638	confocal laser scanning microscopy	1605:1638	confocal laser scanning microscopy	1605:1638	The changes in the bacterial morphology with a disrupted membrane were further confirmed through various imaging techniques like confocal laser scanning microscopy, FESEM, AFM, and 3D digital microscopy.
33464809	5	88	with	loading	918:924	arg1	cCNCs					1003:1007	cCNCs	1003:1007	cCNCs	1003:1007	The efficient loading and interaction of ciE components, specifically cinnamaldehyde, with MFC and cCNCs resulted in developing competent antibacterial surfaces with dense and uniform microstructures.
33464809	5	88	with	loading	918:924	arg1	MFC					995:997	MFC	995:997	MFC	995:997	The efficient loading and interaction of ciE components, specifically cinnamaldehyde, with MFC and cCNCs resulted in developing competent antibacterial surfaces with dense and uniform microstructures.
33464809	9	89	theme	prone	2062:2066	arg1	conditions					2068:2077	real "in use" contamination prone conditions	2034:2077	real "in use" contamination prone conditions	2034:2077	Furthermore, these architectures demonstrated excellent antibacterial performance under real "in use" contamination prone conditions.
33464809	10	90	theme	advanced	2189:2196	arg1	biomaterials					2209:2220	advanced functional biomaterials	2189:2220	advanced functional biomaterials that can be used for assorted biological/healthcare applications such as wound care and antimicrobial filtering units	2189:2338	Hence, this work provides avenues for the application of crude natural extracts in developing novel forms of advanced functional biomaterials that can be used for assorted biological/healthcare applications such as wound care and antimicrobial filtering units.
33464809	6	91	from	concentration	1399:1411	arg1	method					1277:1282	the zone inhibition method	1257:1282	the zone inhibition method	1257:1282	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	6	91	from	concentration	1399:1411	arg1	observation					1302:1312	bacterial growth observation	1285:1312	bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests	1285:1473	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	10	92	theme	biological/healthcare	2252:2272	arg1	care					2301:2304	wound care	2295:2304	wound care	2295:2304	Hence, this work provides avenues for the application of crude natural extracts in developing novel forms of advanced functional biomaterials that can be used for assorted biological/healthcare applications such as wound care and antimicrobial filtering units.
33464809	10	92	theme	biological/healthcare	2252:2272	arg1	units					2334:2338	antimicrobial filtering units	2310:2338	antimicrobial filtering units	2310:2338	Hence, this work provides avenues for the application of crude natural extracts in developing novel forms of advanced functional biomaterials that can be used for assorted biological/healthcare applications such as wound care and antimicrobial filtering units.
33464809	10	92	theme	biological/healthcare	2252:2272	arg1	applications					2274:2285	assorted biological/healthcare applications	2243:2285	assorted biological/healthcare applications such as wound care and antimicrobial filtering units	2243:2338	Hence, this work provides avenues for the application of crude natural extracts in developing novel forms of advanced functional biomaterials that can be used for assorted biological/healthcare applications such as wound care and antimicrobial filtering units.
33464809	6	93	theme	long-term	1119:1127	arg1	activity					1143:1150	Excellent and long-term antimicrobial activity	1105:1150	activity	1143:1150	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	4	94	theme	%	792:792	arg1	improvement					767:777	an improvement	764:777	an improvement of around 106% in compressive strength	764:816	At an optimal concentration of 0.3 wt % cCNCs (i.e., for ciMFC/cCNCs_0.3), an improvement of around 106% in compressive strength and 175% increment in modulus were achieved as compared to pristine MFC architecture.
33464809	4	94	theme	%	792:792	arg1	increment					827:835	175% increment	822:835	175% increment in modulus	822:846	At an optimal concentration of 0.3 wt % cCNCs (i.e., for ciMFC/cCNCs_0.3), an improvement of around 106% in compressive strength and 175% increment in modulus were achieved as compared to pristine MFC architecture.
33464809	0	95	theme	Nanocellulose-Based	14:32	arg1	Architectures					65:77	Microdesigned Nanocellulose-Based Flexible Antibacterial Aerogel Architectures	0:77	Microdesigned Nanocellulose-Based Flexible Antibacterial Aerogel Architectures	0:77	Microdesigned Nanocellulose-Based Flexible Antibacterial Aerogel Architectures Impregnated with Bioactive Cinnamomum cassia.
33464809	4	96	from	improvement	767:777	arg1	strength					809:816	compressive strength	797:816	compressive strength	797:816	At an optimal concentration of 0.3 wt % cCNCs (i.e., for ciMFC/cCNCs_0.3), an improvement of around 106% in compressive strength and 175% increment in modulus were achieved as compared to pristine MFC architecture.
33464809	4	96	from	improvement	767:777	arg1	modulus					840:846	modulus	840:846	modulus	840:846	At an optimal concentration of 0.3 wt % cCNCs (i.e., for ciMFC/cCNCs_0.3), an improvement of around 106% in compressive strength and 175% increment in modulus were achieved as compared to pristine MFC architecture.
33464809	5	97	theme	components	949:958	arg1	interaction					930:940	interaction	930:940	interaction of ciE components, specifically cinnamaldehyde, with MFC and cCNCs	930:1007	The efficient loading and interaction of ciE components, specifically cinnamaldehyde, with MFC and cCNCs resulted in developing competent antibacterial surfaces with dense and uniform microstructures.
33464809	5	97	theme	components	949:958	arg1	loading					918:924	The efficient loading	904:924	The efficient loading	904:924	The efficient loading and interaction of ciE components, specifically cinnamaldehyde, with MFC and cCNCs resulted in developing competent antibacterial surfaces with dense and uniform microstructures.
33464809	6	98	theme	Excellent	1105:1113	arg1	activity					1143:1150	Excellent and long-term antimicrobial activity	1105:1150	activity	1143:1150	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	5	99	with	interaction	930:940	arg1	cCNCs					1003:1007	cCNCs	1003:1007	cCNCs	1003:1007	The efficient loading and interaction of ciE components, specifically cinnamaldehyde, with MFC and cCNCs resulted in developing competent antibacterial surfaces with dense and uniform microstructures.
33464809	5	99	with	interaction	930:940	arg1	MFC					995:997	MFC	995:997	MFC	995:997	The efficient loading and interaction of ciE components, specifically cinnamaldehyde, with MFC and cCNCs resulted in developing competent antibacterial surfaces with dense and uniform microstructures.
33464809	10	100	theme	antimicrobial	2310:2322	arg1	units					2334:2338	antimicrobial filtering units	2310:2338	antimicrobial filtering units	2310:2338	Hence, this work provides avenues for the application of crude natural extracts in developing novel forms of advanced functional biomaterials that can be used for assorted biological/healthcare applications such as wound care and antimicrobial filtering units.
33464809	3	101	theme	cellulose	472:480	arg1	nanocrystals					482:493	carboxylated cellulose nanocrystals	459:493	carboxylated cellulose nanocrystals (cCNCs)	459:501	To this MFC suspension, carboxylated cellulose nanocrystals (cCNCs) were homogeneously mixed and the viscous gel thus obtained was freeze-dried to obtain lightweight and flexible composite aerogel architectures impregnated with ciE, namely, ciMFC/cCNCs.
33464809	3	101	theme	cellulose	472:480	arg1	cCNCs					496:500	cCNCs	496:500	cCNCs	496:500	To this MFC suspension, carboxylated cellulose nanocrystals (cCNCs) were homogeneously mixed and the viscous gel thus obtained was freeze-dried to obtain lightweight and flexible composite aerogel architectures impregnated with ciE, namely, ciMFC/cCNCs.
33464809	0	102	theme	Antibacterial	43:55	arg1	Architectures					65:77	Microdesigned Nanocellulose-Based Flexible Antibacterial Aerogel Architectures	0:77	Microdesigned Nanocellulose-Based Flexible Antibacterial Aerogel Architectures	0:77	Microdesigned Nanocellulose-Based Flexible Antibacterial Aerogel Architectures Impregnated with Bioactive Cinnamomum cassia.
33464809	5	103	theme	competent	1032:1040	arg1	surfaces					1056:1063	competent antibacterial surfaces	1032:1063	competent antibacterial surfaces	1032:1063	The efficient loading and interaction of ciE components, specifically cinnamaldehyde, with MFC and cCNCs resulted in developing competent antibacterial surfaces with dense and uniform microstructures.
33464809	4	104	theme	%	825:825	arg1	increment					827:835	175% increment	822:835	175% increment in modulus	822:846	At an optimal concentration of 0.3 wt % cCNCs (i.e., for ciMFC/cCNCs_0.3), an improvement of around 106% in compressive strength and 175% increment in modulus were achieved as compared to pristine MFC architecture.
33464809	1	105	theme	nanocellulose-based	264:282	arg1	architectures					298:310	nanocellulose-based antibacterial architectures	264:310	nanocellulose-based antibacterial architectures	264:310	This work is strategically premeditated to study the potential of a herbal medicinal product as a natural bioactive ingredient to generate nanocellulose-based antibacterial architectures.
33464809	7	106	theme	various	1573:1579	arg1	techniques					1589:1598	various imaging techniques	1573:1598	various imaging techniques like confocal laser scanning microscopy, FESEM, AFM, and 3D digital microscopy	1573:1677	The changes in the bacterial morphology with a disrupted membrane were further confirmed through various imaging techniques like confocal laser scanning microscopy, FESEM, AFM, and 3D digital microscopy.
33464809	3	107	theme	MFC	443:445	arg1	suspension					447:456	this MFC suspension	438:456	this MFC suspension	438:456	To this MFC suspension, carboxylated cellulose nanocrystals (cCNCs) were homogeneously mixed and the viscous gel thus obtained was freeze-dried to obtain lightweight and flexible composite aerogel architectures impregnated with ciE, namely, ciMFC/cCNCs.
33464809	3	108	theme	viscous	536:542	arg1	gel					544:546	the viscous gel	532:546	the viscous gel thus obtained	532:560	To this MFC suspension, carboxylated cellulose nanocrystals (cCNCs) were homogeneously mixed and the viscous gel thus obtained was freeze-dried to obtain lightweight and flexible composite aerogel architectures impregnated with ciE, namely, ciMFC/cCNCs.
33464809	8	109	theme	composite	1688:1696	arg1	architecture					1698:1709	The dry composite architecture	1680:1709	The dry composite architecture	1680:1709	The dry composite architecture showed the persuasive capability of suppressing the growth of airborne bacteria, which in combination with antibacterial efficiency in the wet state is considered as an imperative aspect for a material to act as the novel biomaterial.
33464809	6	110	theme	architectures	1169:1181	arg1	activity					1143:1150	Excellent and long-term antimicrobial activity	1105:1150	activity	1143:1150	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	4	111	theme	compressive	797:807	arg1	strength					809:816	compressive strength	797:816	compressive strength	797:816	At an optimal concentration of 0.3 wt % cCNCs (i.e., for ciMFC/cCNCs_0.3), an improvement of around 106% in compressive strength and 175% increment in modulus were achieved as compared to pristine MFC architecture.
33464809	5	112	theme	uniform	1080:1086	arg1	microstructures					1088:1102	dense and uniform microstructures	1070:1102	dense and uniform microstructures	1070:1102	The efficient loading and interaction of ciE components, specifically cinnamaldehyde, with MFC and cCNCs resulted in developing competent antibacterial surfaces with dense and uniform microstructures.
33464809	6	113	dep	OD600	1317:1321	arg1	tests					1469:1473	viability tests	1459:1473	viability tests	1459:1473	Excellent and long-term antimicrobial activity of the optimized architectures (ciMFC/cCNCs_0.3) was confirmed through various antibacterial assays like the zone inhibition method, bacterial growth observation at OD600, minimum inhibitory concentration (MIC, here 1 mg/mL), minimum bactericidal concentration (MBC, here 3-5 mg/mL), and Live/Dead BacLight viability tests.
33464809	8	114	theme	persuasive	1722:1731	arg1	capability					1733:1742	the persuasive capability	1718:1742	the persuasive capability of suppressing the growth of airborne bacteria, which in combination with antibacterial efficiency in the wet state is considered as an imperative aspect for a material to act as the novel biomaterial	1718:1943	The dry composite architecture showed the persuasive capability of suppressing the growth of airborne bacteria, which in combination with antibacterial efficiency in the wet state is considered as an imperative aspect for a material to act as the novel biomaterial.
33464809	7	115	with	morphology	1505:1514	arg1	membrane					1533:1540	a disrupted membrane	1521:1540	a disrupted membrane	1521:1540	The changes in the bacterial morphology with a disrupted membrane were further confirmed through various imaging techniques like confocal laser scanning microscopy, FESEM, AFM, and 3D digital microscopy.
33580179	6	0	theme	inhibitory	829:838	arg1	activity					840:847	the inhibitory activity	825:847	the inhibitory activity of Cst-3 towards papain	825:871	We further discovered that HS-binding severely impairs the inhibitory activity of Cst-3 towards papain, suggesting the interaction could actively regulate Cst-3 activity.
33580179	1	1	theme	proteases	127:135	arg1	inhibitor					105:113	a potent inhibitor	96:113	a potent inhibitor of cysteine proteases with diverse biological functions	96:169	Cystatin C (Cst-3) is a potent inhibitor of cysteine proteases with diverse biological functions.
33580179	1	1	theme	proteases	127:135	arg1	C					83:83	Cystatin C	74:83	Cystatin C (Cst-3)	74:91	Cystatin C (Cst-3) is a potent inhibitor of cysteine proteases with diverse biological functions.
33580179	5	2	theme	site	696:699	arg1	unique					733:738	unique	733:738	unique	733:738	The composition of the HS-binding site by two highly dynamic halves is unique in known HS-binding proteins.
33580179	5	2	theme	site	696:699	arg1	composition					666:676	The composition	662:676	The composition of the HS-binding site by two highly dynamic halves	662:728	The composition of the HS-binding site by two highly dynamic halves is unique in known HS-binding proteins.
33580179	4	3	from	regions	553:559	arg1	Cst-3					564:568	Cst-3	564:568	Cst-3	564:568	We discovered that Cst-3 is a HS-binding protein only at acidic pH. By NMR and site-directed mutagenesis, we identified two HS binding regions in Cst-3: the highly dynamic N-terminal segment and a flexible region located between residue 70-94.
33580179	4	4	dep	pH.	482:484	arg1	identified					527:536	identified	527:536	identified two HS binding regions in Cst-3: the highly dynamic N-terminal segment and a flexible region located between residue 70-94	527:659	We discovered that Cst-3 is a HS-binding protein only at acidic pH. By NMR and site-directed mutagenesis, we identified two HS binding regions in Cst-3: the highly dynamic N-terminal segment and a flexible region located between residue 70-94.
33580179	6	5	theme	Cst-3	852:856	arg1	activity					840:847	the inhibitory activity	825:847	the inhibitory activity of Cst-3 towards papain	825:871	We further discovered that HS-binding severely impairs the inhibitory activity of Cst-3 towards papain, suggesting the interaction could actively regulate Cst-3 activity.
33580179	4	6	from	region	624:629	arg1	Cst-3					564:568	Cst-3	564:568	Cst-3	564:568	We discovered that Cst-3 is a HS-binding protein only at acidic pH. By NMR and site-directed mutagenesis, we identified two HS binding regions in Cst-3: the highly dynamic N-terminal segment and a flexible region located between residue 70-94.
33580179	4	7	theme	binding	545:551	arg1	regions					553:559	two HS binding regions	538:559	two HS binding regions in Cst-3	538:568	We discovered that Cst-3 is a HS-binding protein only at acidic pH. By NMR and site-directed mutagenesis, we identified two HS binding regions in Cst-3: the highly dynamic N-terminal segment and a flexible region located between residue 70-94.
33580179	5	8	from	proteins	760:767	arg1	unique					733:738	unique	733:738	unique	733:738	The composition of the HS-binding site by two highly dynamic halves is unique in known HS-binding proteins.
33580179	5	8	from	proteins	760:767	arg1	composition					666:676	The composition	662:676	The composition of the HS-binding site by two highly dynamic halves	662:728	The composition of the HS-binding site by two highly dynamic halves is unique in known HS-binding proteins.
33580179	7	9	theme	physiological	1053:1065	arg1	relevance					1067:1075	the physiological relevance	1049:1075	the physiological relevance of our discovery	1049:1092	Using murine bone tissues, we showed that Cst-3 interacts with bone matrix HS at low pH, again highlighting the physiological relevance of our discovery.
33580179	4	10	theme	HS	542:543	arg1	regions					553:559	two HS binding regions	538:559	two HS binding regions in Cst-3	538:568	We discovered that Cst-3 is a HS-binding protein only at acidic pH. By NMR and site-directed mutagenesis, we identified two HS binding regions in Cst-3: the highly dynamic N-terminal segment and a flexible region located between residue 70-94.
33580179	5	11	theme	HS-binding	685:694	arg1	site					696:699	the HS-binding site	681:699	the HS-binding site	681:699	The composition of the HS-binding site by two highly dynamic halves is unique in known HS-binding proteins.
33580179	1	12	theme	diverse	142:148	arg1	functions					161:169	diverse biological functions	142:169	diverse biological functions	142:169	Cystatin C (Cst-3) is a potent inhibitor of cysteine proteases with diverse biological functions.
33580179	4	13	from	pH.	482:484	arg1	Cst-3					437:441	Cst-3	437:441	Cst-3	437:441	We discovered that Cst-3 is a HS-binding protein only at acidic pH. By NMR and site-directed mutagenesis, we identified two HS binding regions in Cst-3: the highly dynamic N-terminal segment and a flexible region located between residue 70-94.
33580179	4	13	from	pH.	482:484	arg1	protein					459:465	a HS-binding protein	446:465	a HS-binding protein	446:465	We discovered that Cst-3 is a HS-binding protein only at acidic pH. By NMR and site-directed mutagenesis, we identified two HS binding regions in Cst-3: the highly dynamic N-terminal segment and a flexible region located between residue 70-94.
33580179	4	14	theme	HS-binding	448:457	arg1	Cst-3					437:441	Cst-3	437:441	Cst-3	437:441	We discovered that Cst-3 is a HS-binding protein only at acidic pH. By NMR and site-directed mutagenesis, we identified two HS binding regions in Cst-3: the highly dynamic N-terminal segment and a flexible region located between residue 70-94.
33580179	4	14	theme	HS-binding	448:457	arg1	protein					459:465	a HS-binding protein	446:465	a HS-binding protein	446:465	We discovered that Cst-3 is a HS-binding protein only at acidic pH. By NMR and site-directed mutagenesis, we identified two HS binding regions in Cst-3: the highly dynamic N-terminal segment and a flexible region located between residue 70-94.
33580179	1	15	with	inhibitor	105:113	arg1	functions					161:169	diverse biological functions	142:169	diverse biological functions	142:169	Cystatin C (Cst-3) is a potent inhibitor of cysteine proteases with diverse biological functions.
33580179	1	16	theme	Cystatin	74:81	arg1	inhibitor					105:113	a potent inhibitor	96:113	a potent inhibitor of cysteine proteases with diverse biological functions	96:169	Cystatin C (Cst-3) is a potent inhibitor of cysteine proteases with diverse biological functions.
33580179	1	16	theme	Cystatin	74:81	arg1	Cst-3					86:90	Cst-3	86:90	Cst-3	86:90	Cystatin C (Cst-3) is a potent inhibitor of cysteine proteases with diverse biological functions.
33580179	1	16	theme	Cystatin	74:81	arg1	C					83:83	Cystatin C	74:83	Cystatin C (Cst-3)	74:91	Cystatin C (Cst-3) is a potent inhibitor of cysteine proteases with diverse biological functions.
33580179	1	17	theme	biological	150:159	arg1	functions					161:169	diverse biological functions	142:169	diverse biological functions	142:169	Cystatin C (Cst-3) is a potent inhibitor of cysteine proteases with diverse biological functions.
33580179	5	18	theme	known	743:747	arg1	proteins					760:767	known HS-binding proteins	743:767	known HS-binding proteins	743:767	The composition of the HS-binding site by two highly dynamic halves is unique in known HS-binding proteins.
33580179	5	19	theme	dynamic	715:721	arg1	halves					723:728	two highly dynamic halves	704:728	two highly dynamic halves	704:728	The composition of the HS-binding site by two highly dynamic halves is unique in known HS-binding proteins.
33580179	4	20	from	segment	601:607	arg1	Cst-3					564:568	Cst-3	564:568	Cst-3	564:568	We discovered that Cst-3 is a HS-binding protein only at acidic pH. By NMR and site-directed mutagenesis, we identified two HS binding regions in Cst-3: the highly dynamic N-terminal segment and a flexible region located between residue 70-94.
33580179	4	21	theme	flexible	615:622	arg1	region					624:629	a flexible region	613:629	a flexible region located between residue 70-94	613:659	We discovered that Cst-3 is a HS-binding protein only at acidic pH. By NMR and site-directed mutagenesis, we identified two HS binding regions in Cst-3: the highly dynamic N-terminal segment and a flexible region located between residue 70-94.
33580179	0	22	theme	pH-dependent	0:11	arg1	interactions					25:36	pH-dependent and dynamic interactions	0:36	pH-dependent and dynamic interactions of cystatin C with heparan sulfate	0:71	pH-dependent and dynamic interactions of cystatin C with heparan sulfate.
33580179	7	23	from	pH	1026:1027	arg1	HS					1016:1017	bone matrix HS	1004:1017	bone matrix HS at low pH	1004:1027	Using murine bone tissues, we showed that Cst-3 interacts with bone matrix HS at low pH, again highlighting the physiological relevance of our discovery.
33580179	5	24	theme	HS-binding	749:758	arg1	proteins					760:767	known HS-binding proteins	743:767	known HS-binding proteins	743:767	The composition of the HS-binding site by two highly dynamic halves is unique in known HS-binding proteins.
33580179	3	25	theme	heparan	353:359	arg1	HS					370:371	HS	370:371	HS	370:371	Here we investigated the interaction between Cst-3 and heparan sulfate (HS), a major component of extracellular matrix.
33580179	3	25	theme	heparan	353:359	arg1	sulfate					361:367	heparan sulfate	353:367	heparan sulfate (HS)	353:372	Here we investigated the interaction between Cst-3 and heparan sulfate (HS), a major component of extracellular matrix.
33580179	4	26	theme	site-directed	497:509	arg1	mutagenesis					511:521	site-directed mutagenesis	497:521	site-directed mutagenesis	497:521	We discovered that Cst-3 is a HS-binding protein only at acidic pH. By NMR and site-directed mutagenesis, we identified two HS binding regions in Cst-3: the highly dynamic N-terminal segment and a flexible region located between residue 70-94.
33580179	0	27	theme	dynamic	17:23	arg1	interactions					25:36	pH-dependent and dynamic interactions	0:36	pH-dependent and dynamic interactions of cystatin C with heparan sulfate	0:71	pH-dependent and dynamic interactions of cystatin C with heparan sulfate.
33580179	6	28	theme	Cst-3	925:929	arg1	activity					931:938	Cst-3 activity	925:938	Cst-3 activity	925:938	We further discovered that HS-binding severely impairs the inhibitory activity of Cst-3 towards papain, suggesting the interaction could actively regulate Cst-3 activity.
33580179	7	29	theme	low	1022:1024	arg1	pH					1026:1027	low pH	1022:1027	low pH	1022:1027	Using murine bone tissues, we showed that Cst-3 interacts with bone matrix HS at low pH, again highlighting the physiological relevance of our discovery.
33580179	0	30	theme	cystatin	41:48	arg1	C					50:50	cystatin C	41:50	cystatin C	41:50	pH-dependent and dynamic interactions of cystatin C with heparan sulfate.
33580179	7	31	theme	matrix	1009:1014	arg1	HS					1016:1017	bone matrix HS	1004:1017	bone matrix HS at low pH	1004:1027	Using murine bone tissues, we showed that Cst-3 interacts with bone matrix HS at low pH, again highlighting the physiological relevance of our discovery.
33580179	4	32	theme	N-terminal	590:599	arg1	segment					601:607	the highly dynamic N-terminal segment	571:607	the highly dynamic N-terminal segment	571:607	We discovered that Cst-3 is a HS-binding protein only at acidic pH. By NMR and site-directed mutagenesis, we identified two HS binding regions in Cst-3: the highly dynamic N-terminal segment and a flexible region located between residue 70-94.
33580179	3	33	theme	major	377:381	arg1	component					383:391	a major component	375:391	a major component of extracellular matrix	375:415	Here we investigated the interaction between Cst-3 and heparan sulfate (HS), a major component of extracellular matrix.
33580179	3	33	theme	major	377:381	arg1	Cst-3					343:347	Cst-3	343:347	Cst-3	343:347	Here we investigated the interaction between Cst-3 and heparan sulfate (HS), a major component of extracellular matrix.
33580179	2	34	theme	potential	199:207	arg1	interaction					209:219	the potential interaction	195:219	the potential interaction between Cst-3 and extracellular matrix components	195:269	As a secreted protein, the potential interaction between Cst-3 and extracellular matrix components has not been well studied.
33580179	2	34	theme	potential	199:207	arg1	protein					186:192	a secreted protein	175:192	a secreted protein	175:192	As a secreted protein, the potential interaction between Cst-3 and extracellular matrix components has not been well studied.
33580179	0	35	with	interactions	25:36	arg1	sulfate					65:71	heparan sulfate	57:71	heparan sulfate	57:71	pH-dependent and dynamic interactions of cystatin C with heparan sulfate.
33580179	4	36	theme	dynamic	582:588	arg1	segment					601:607	the highly dynamic N-terminal segment	571:607	the highly dynamic N-terminal segment	571:607	We discovered that Cst-3 is a HS-binding protein only at acidic pH. By NMR and site-directed mutagenesis, we identified two HS binding regions in Cst-3: the highly dynamic N-terminal segment and a flexible region located between residue 70-94.
33580179	3	37	theme	extracellular	396:408	arg1	matrix					410:415	extracellular matrix	396:415	extracellular matrix	396:415	Here we investigated the interaction between Cst-3 and heparan sulfate (HS), a major component of extracellular matrix.
33580179	0	38	theme	C	50:50	arg1	interactions					25:36	pH-dependent and dynamic interactions	0:36	pH-dependent and dynamic interactions of cystatin C with heparan sulfate	0:71	pH-dependent and dynamic interactions of cystatin C with heparan sulfate.
33580179	7	39	theme	murine	947:952	arg1	tissues					959:965	murine bone tissues	947:965	murine bone tissues	947:965	Using murine bone tissues, we showed that Cst-3 interacts with bone matrix HS at low pH, again highlighting the physiological relevance of our discovery.
33580179	7	40	theme	bone	1004:1007	arg1	HS					1016:1017	bone matrix HS	1004:1017	bone matrix HS at low pH	1004:1027	Using murine bone tissues, we showed that Cst-3 interacts with bone matrix HS at low pH, again highlighting the physiological relevance of our discovery.
33580179	7	41	theme	discovery	1084:1092	arg1	relevance					1067:1075	the physiological relevance	1049:1075	the physiological relevance of our discovery	1049:1092	Using murine bone tissues, we showed that Cst-3 interacts with bone matrix HS at low pH, again highlighting the physiological relevance of our discovery.
33580179	2	42	theme	secreted	177:184	arg1	interaction					209:219	the potential interaction	195:219	the potential interaction between Cst-3 and extracellular matrix components	195:269	As a secreted protein, the potential interaction between Cst-3 and extracellular matrix components has not been well studied.
33580179	2	42	theme	secreted	177:184	arg1	protein					186:192	a secreted protein	175:192	a secreted protein	175:192	As a secreted protein, the potential interaction between Cst-3 and extracellular matrix components has not been well studied.
33580179	2	43	theme	extracellular	239:251	arg1	components					260:269	extracellular matrix components	239:269	extracellular matrix components	239:269	As a secreted protein, the potential interaction between Cst-3 and extracellular matrix components has not been well studied.
33580179	1	44	theme	potent	98:103	arg1	inhibitor					105:113	a potent inhibitor	96:113	a potent inhibitor of cysteine proteases with diverse biological functions	96:169	Cystatin C (Cst-3) is a potent inhibitor of cysteine proteases with diverse biological functions.
33580179	1	44	theme	potent	98:103	arg1	C					83:83	Cystatin C	74:83	Cystatin C (Cst-3)	74:91	Cystatin C (Cst-3) is a potent inhibitor of cysteine proteases with diverse biological functions.
33580179	2	45	theme	matrix	253:258	arg1	components					260:269	extracellular matrix components	239:269	extracellular matrix components	239:269	As a secreted protein, the potential interaction between Cst-3 and extracellular matrix components has not been well studied.
33580179	7	46	theme	bone	954:957	arg1	tissues					959:965	murine bone tissues	947:965	murine bone tissues	947:965	Using murine bone tissues, we showed that Cst-3 interacts with bone matrix HS at low pH, again highlighting the physiological relevance of our discovery.
33580179	4	47	theme	located	631:637	arg1	region					624:629	a flexible region	613:629	a flexible region located between residue 70-94	613:659	We discovered that Cst-3 is a HS-binding protein only at acidic pH. By NMR and site-directed mutagenesis, we identified two HS binding regions in Cst-3: the highly dynamic N-terminal segment and a flexible region located between residue 70-94.
33580179	3	48	theme	matrix	410:415	arg1	component					383:391	a major component	375:391	a major component of extracellular matrix	375:415	Here we investigated the interaction between Cst-3 and heparan sulfate (HS), a major component of extracellular matrix.
33580179	3	48	theme	matrix	410:415	arg1	Cst-3					343:347	Cst-3	343:347	Cst-3	343:347	Here we investigated the interaction between Cst-3 and heparan sulfate (HS), a major component of extracellular matrix.
33580179	0	49	theme	heparan	57:63	arg1	sulfate					65:71	heparan sulfate	57:71	heparan sulfate	57:71	pH-dependent and dynamic interactions of cystatin C with heparan sulfate.
33580179	5	50	from	unique	733:738	arg1	proteins					760:767	known HS-binding proteins	743:767	known HS-binding proteins	743:767	The composition of the HS-binding site by two highly dynamic halves is unique in known HS-binding proteins.
33580179	1	51	theme	cysteine	118:125	arg1	proteases					127:135	cysteine proteases	118:135	cysteine proteases	118:135	Cystatin C (Cst-3) is a potent inhibitor of cysteine proteases with diverse biological functions.
34314794	0	0	from	fabrication	11:21	arg1	engineering					104:114	dental tissue engineering	90:114	dental tissue engineering	90:114	Design and fabrication of M-SAPO-34/chitosan scaffolds and evaluation of their effects on dental tissue engineering.
34314794	1	1	theme	therapeutic	158:168	arg1	bio-composites					170:183	innovative therapeutic bio-composites	147:183	innovative therapeutic bio-composites that enhance odontogenic and osteogenic differentiation of human dental pulp-derived mesenchymal stem cells (h-DPSCs) in-vitro regeneration	147:323	This research aimed to design innovative therapeutic bio-composites that enhance odontogenic and osteogenic differentiation of human dental pulp-derived mesenchymal stem cells (h-DPSCs) in-vitro regeneration.
34314794	1	2	theme	cells	287:291	arg1	differentiation					225:239	odontogenic and osteogenic differentiation	198:239	odontogenic and osteogenic differentiation of human dental pulp-derived mesenchymal stem cells	198:291	This research aimed to design innovative therapeutic bio-composites that enhance odontogenic and osteogenic differentiation of human dental pulp-derived mesenchymal stem cells (h-DPSCs) in-vitro regeneration.
34314794	0	3	theme	effects	79:85	arg1	fabrication					11:21	fabrication	11:21	fabrication	11:21	Design and fabrication of M-SAPO-34/chitosan scaffolds and evaluation of their effects on dental tissue engineering.
34314794	0	3	theme	effects	79:85	arg1	Design					0:5	Design	0:5	Design	0:5	Design and fabrication of M-SAPO-34/chitosan scaffolds and evaluation of their effects on dental tissue engineering.
34314794	0	3	theme	effects	79:85	arg1	evaluation					59:68	evaluation	59:68	evaluation of their effects on dental tissue engineering	59:114	Design and fabrication of M-SAPO-34/chitosan scaffolds and evaluation of their effects on dental tissue engineering.
34314794	6	4	theme	moderate	1187:1194	arg1	effect					1196:1201	moderate effect	1187:1201	moderate effect on cell proliferation	1187:1223	Cell tests demonstrated that Ca-SAPO-34/CS scaffold clearly displayed a positive effect on differentiation of hDPSCs into osteogenic/odontogenic cells and moderate effect on cell proliferation.
34314794	2	5	theme	chitosan	384:391	arg1	nanoparticles					450:462	chitosan, Ca-SAPO-34 monometallic and/or Fe-Ca-SAPO-34 bimetallic nanoparticles	384:462	chitosan, Ca-SAPO-34 monometallic and/or Fe-Ca-SAPO-34 bimetallic nanoparticles by freeze-drying technique	384:489	Herein, we report the fabrication of scaffolds containing chitosan, Ca-SAPO-34 monometallic and/or Fe-Ca-SAPO-34 bimetallic nanoparticles by freeze-drying technique.
34314794	8	6	contain	has	1591:1593	arg2	biomaterial					1621:1631	biomaterial	1621:1631	biomaterial	1621:1631	Alizarin red, Alkaline phosphatase and QRT-PCR results showed that Fe-Ca-loaded SAPO-34/CS can lead to osteoblast/odontoblast differentiation in DPSCs through the up-regulation of related genes, thus indicating that Fe-Ca-SAPO-34/CS has remarkable prospects as a biomaterial for hard tissue engineering.
34314794	8	6	contain	has	1591:1593	arg2	prospects					1606:1614	remarkable prospects	1595:1614	remarkable prospects	1595:1614	Alizarin red, Alkaline phosphatase and QRT-PCR results showed that Fe-Ca-loaded SAPO-34/CS can lead to osteoblast/odontoblast differentiation in DPSCs through the up-regulation of related genes, thus indicating that Fe-Ca-SAPO-34/CS has remarkable prospects as a biomaterial for hard tissue engineering.
34314794	8	6	contain	has	1591:1593	arg1	Fe-Ca-SAPO-34/CS					1574:1589	Fe-Ca-SAPO-34/CS	1574:1589	Fe-Ca-SAPO-34/CS	1574:1589	Alizarin red, Alkaline phosphatase and QRT-PCR results showed that Fe-Ca-loaded SAPO-34/CS can lead to osteoblast/odontoblast differentiation in DPSCs through the up-regulation of related genes, thus indicating that Fe-Ca-SAPO-34/CS has remarkable prospects as a biomaterial for hard tissue engineering.
34314794	5	7	theme	cell	987:990	arg1	proliferation					992:1004	cell proliferation	987:1004	cell proliferation	987:1004	Cell viability, cell adhesion and cytotoxicity of Ca-SAPO-34/CS and Fe-Ca-SAPO-34 scaffolds were investigated by MTT assay and SEM on h-DPSCs which revealed cell proliferation no toxicity on scaffolds.
34314794	0	8	theme	tissue	97:102	arg1	engineering					104:114	dental tissue engineering	90:114	dental tissue engineering	90:114	Design and fabrication of M-SAPO-34/chitosan scaffolds and evaluation of their effects on dental tissue engineering.
34314794	0	9	from	Design	0:5	arg1	engineering					104:114	dental tissue engineering	90:114	dental tissue engineering	90:114	Design and fabrication of M-SAPO-34/chitosan scaffolds and evaluation of their effects on dental tissue engineering.
34314794	5	10	from	SEM	957:959	arg1	h-DPSCs					964:970	h-DPSCs	964:970	h-DPSCs which revealed cell proliferation no toxicity on scaffolds	964:1029	Cell viability, cell adhesion and cytotoxicity of Ca-SAPO-34/CS and Fe-Ca-SAPO-34 scaffolds were investigated by MTT assay and SEM on h-DPSCs which revealed cell proliferation no toxicity on scaffolds.
34314794	0	11	theme	dental	90:95	arg1	engineering					104:114	dental tissue engineering	90:114	dental tissue engineering	90:114	Design and fabrication of M-SAPO-34/chitosan scaffolds and evaluation of their effects on dental tissue engineering.
34314794	0	12	from	evaluation	59:68	arg1	engineering					104:114	dental tissue engineering	90:114	dental tissue engineering	90:114	Design and fabrication of M-SAPO-34/chitosan scaffolds and evaluation of their effects on dental tissue engineering.
34314794	7	13	theme	hDPSCs	1319:1324	arg1	differentiation					1341:1355	osteogenic differentiation	1330:1355	osteogenic differentiation	1330:1355	Moreover, the incorporation of Fe2O3 to Ca-SAPO-34/CS scaffold promoted the proliferation of hDPSCs and osteogenic differentiation.
34314794	7	13	theme	hDPSCs	1319:1324	arg1	proliferation					1302:1314	the proliferation	1298:1314	the proliferation of hDPSCs	1298:1324	Moreover, the incorporation of Fe2O3 to Ca-SAPO-34/CS scaffold promoted the proliferation of hDPSCs and osteogenic differentiation.
34314794	3	14	theme	SEM	585:587	arg1	methods					598:604	ICP-AES, FT-IR, XRD, TGA, TEM, BET, SEM, and EDS methods	549:604	ICP-AES, FT-IR, XRD, TGA, TEM, BET, SEM, and EDS methods	549:604	The scaffolds and nanoparticles were characterized using ICP-AES, FT-IR, XRD, TGA, TEM, BET, SEM, and EDS methods.
34314794	3	15	theme	FT-IR	558:562	arg1	methods					598:604	ICP-AES, FT-IR, XRD, TGA, TEM, BET, SEM, and EDS methods	549:604	ICP-AES, FT-IR, XRD, TGA, TEM, BET, SEM, and EDS methods	549:604	The scaffolds and nanoparticles were characterized using ICP-AES, FT-IR, XRD, TGA, TEM, BET, SEM, and EDS methods.
34314794	4	16	theme	swelling	734:741	arg1	ratio					743:747	swelling ratio	734:747	swelling ratio	734:747	The effects of SAPO-34 and nanoparticles were investigated by changes on the physicochemical properties of scaffolds including swelling ratio, density, porosity, bio-degradation, mechanical behavior, and biomineralization.
34314794	8	17	theme	genes	1546:1550	arg1	up-regulation					1521:1533	the up-regulation	1517:1533	the up-regulation of related genes	1517:1550	Alizarin red, Alkaline phosphatase and QRT-PCR results showed that Fe-Ca-loaded SAPO-34/CS can lead to osteoblast/odontoblast differentiation in DPSCs through the up-regulation of related genes, thus indicating that Fe-Ca-SAPO-34/CS has remarkable prospects as a biomaterial for hard tissue engineering.
34314794	8	18	theme	red	1367:1369	arg1	Alizarin					1358:1365	Alizarin red	1358:1369	Alizarin red	1358:1369	Alizarin red, Alkaline phosphatase and QRT-PCR results showed that Fe-Ca-loaded SAPO-34/CS can lead to osteoblast/odontoblast differentiation in DPSCs through the up-regulation of related genes, thus indicating that Fe-Ca-SAPO-34/CS has remarkable prospects as a biomaterial for hard tissue engineering.
34314794	5	19	theme	Ca-SAPO-34/CS	880:892	arg1	scaffolds					912:920	Ca-SAPO-34/CS and Fe-Ca-SAPO-34 scaffolds	880:920	Ca-SAPO-34/CS and Fe-Ca-SAPO-34 scaffolds	880:920	Cell viability, cell adhesion and cytotoxicity of Ca-SAPO-34/CS and Fe-Ca-SAPO-34 scaffolds were investigated by MTT assay and SEM on h-DPSCs which revealed cell proliferation no toxicity on scaffolds.
34314794	2	20	theme	Fe-Ca-SAPO-34	425:437	arg1	bimetallic					439:448	Fe-Ca-SAPO-34 bimetallic	425:448	Fe-Ca-SAPO-34 bimetallic	425:448	Herein, we report the fabrication of scaffolds containing chitosan, Ca-SAPO-34 monometallic and/or Fe-Ca-SAPO-34 bimetallic nanoparticles by freeze-drying technique.
34314794	4	21	theme	physicochemical	684:698	arg1	properties					700:709	the physicochemical properties	680:709	the physicochemical properties of scaffolds including swelling ratio, density, porosity, bio-degradation, mechanical behavior, and biomineralization	680:827	The effects of SAPO-34 and nanoparticles were investigated by changes on the physicochemical properties of scaffolds including swelling ratio, density, porosity, bio-degradation, mechanical behavior, and biomineralization.
34314794	6	22	theme	Ca-SAPO-34/CS	1061:1073	arg1	scaffold					1075:1082	Ca-SAPO-34/CS scaffold	1061:1082	Ca-SAPO-34/CS scaffold	1061:1082	Cell tests demonstrated that Ca-SAPO-34/CS scaffold clearly displayed a positive effect on differentiation of hDPSCs into osteogenic/odontogenic cells and moderate effect on cell proliferation.
34314794	6	23	from	effect	1113:1118	arg1	differentiation					1123:1137	differentiation	1123:1137	differentiation of hDPSCs into osteogenic/odontogenic cells	1123:1181	Cell tests demonstrated that Ca-SAPO-34/CS scaffold clearly displayed a positive effect on differentiation of hDPSCs into osteogenic/odontogenic cells and moderate effect on cell proliferation.
34314794	6	23	from	effect	1113:1118	arg1	proliferation					1211:1223	cell proliferation	1206:1223	cell proliferation	1206:1223	Cell tests demonstrated that Ca-SAPO-34/CS scaffold clearly displayed a positive effect on differentiation of hDPSCs into osteogenic/odontogenic cells and moderate effect on cell proliferation.
34314794	3	24	theme	BET	580:582	arg1	methods					598:604	ICP-AES, FT-IR, XRD, TGA, TEM, BET, SEM, and EDS methods	549:604	ICP-AES, FT-IR, XRD, TGA, TEM, BET, SEM, and EDS methods	549:604	The scaffolds and nanoparticles were characterized using ICP-AES, FT-IR, XRD, TGA, TEM, BET, SEM, and EDS methods.
34314794	2	25	theme	bimetallic	439:448	arg1	nanoparticles					450:462	chitosan, Ca-SAPO-34 monometallic and/or Fe-Ca-SAPO-34 bimetallic nanoparticles	384:462	chitosan, Ca-SAPO-34 monometallic and/or Fe-Ca-SAPO-34 bimetallic nanoparticles by freeze-drying technique	384:489	Herein, we report the fabrication of scaffolds containing chitosan, Ca-SAPO-34 monometallic and/or Fe-Ca-SAPO-34 bimetallic nanoparticles by freeze-drying technique.
34314794	5	26	theme	cell	846:849	arg1	adhesion					851:858	cell adhesion	846:858	cell adhesion	846:858	Cell viability, cell adhesion and cytotoxicity of Ca-SAPO-34/CS and Fe-Ca-SAPO-34 scaffolds were investigated by MTT assay and SEM on h-DPSCs which revealed cell proliferation no toxicity on scaffolds.
34314794	3	27	theme	TEM	575:577	arg1	methods					598:604	ICP-AES, FT-IR, XRD, TGA, TEM, BET, SEM, and EDS methods	549:604	ICP-AES, FT-IR, XRD, TGA, TEM, BET, SEM, and EDS methods	549:604	The scaffolds and nanoparticles were characterized using ICP-AES, FT-IR, XRD, TGA, TEM, BET, SEM, and EDS methods.
34314794	6	28	theme	osteogenic/odontogenic	1154:1175	arg1	cells					1177:1181	osteogenic/odontogenic cells	1154:1181	osteogenic/odontogenic cells	1154:1181	Cell tests demonstrated that Ca-SAPO-34/CS scaffold clearly displayed a positive effect on differentiation of hDPSCs into osteogenic/odontogenic cells and moderate effect on cell proliferation.
34314794	6	29	theme	Cell	1032:1035	arg1	tests					1037:1041	Cell tests	1032:1041	Cell tests	1032:1041	Cell tests demonstrated that Ca-SAPO-34/CS scaffold clearly displayed a positive effect on differentiation of hDPSCs into osteogenic/odontogenic cells and moderate effect on cell proliferation.
34314794	3	30	theme	EDS	594:596	arg1	methods					598:604	ICP-AES, FT-IR, XRD, TGA, TEM, BET, SEM, and EDS methods	549:604	ICP-AES, FT-IR, XRD, TGA, TEM, BET, SEM, and EDS methods	549:604	The scaffolds and nanoparticles were characterized using ICP-AES, FT-IR, XRD, TGA, TEM, BET, SEM, and EDS methods.
34314794	6	31	theme	hDPSCs	1142:1147	arg1	differentiation					1123:1137	differentiation	1123:1137	differentiation of hDPSCs into osteogenic/odontogenic cells	1123:1181	Cell tests demonstrated that Ca-SAPO-34/CS scaffold clearly displayed a positive effect on differentiation of hDPSCs into osteogenic/odontogenic cells and moderate effect on cell proliferation.
34314794	1	32	theme	odontogenic	198:208	arg1	differentiation					225:239	odontogenic and osteogenic differentiation	198:239	odontogenic and osteogenic differentiation of human dental pulp-derived mesenchymal stem cells	198:291	This research aimed to design innovative therapeutic bio-composites that enhance odontogenic and osteogenic differentiation of human dental pulp-derived mesenchymal stem cells (h-DPSCs) in-vitro regeneration.
34314794	7	33	theme	Ca-SAPO-34/CS	1266:1278	arg1	scaffold					1280:1287	Ca-SAPO-34/CS scaffold	1266:1287	Ca-SAPO-34/CS scaffold	1266:1287	Moreover, the incorporation of Fe2O3 to Ca-SAPO-34/CS scaffold promoted the proliferation of hDPSCs and osteogenic differentiation.
34314794	1	34	theme	in-vitro	303:310	arg1	regeneration					312:323	in-vitro regeneration	303:323	in-vitro regeneration	303:323	This research aimed to design innovative therapeutic bio-composites that enhance odontogenic and osteogenic differentiation of human dental pulp-derived mesenchymal stem cells (h-DPSCs) in-vitro regeneration.
34314794	5	35	theme	scaffolds	912:920	arg1	viability					835:843	Cell viability	830:843	Cell viability	830:843	Cell viability, cell adhesion and cytotoxicity of Ca-SAPO-34/CS and Fe-Ca-SAPO-34 scaffolds were investigated by MTT assay and SEM on h-DPSCs which revealed cell proliferation no toxicity on scaffolds.
34314794	5	35	theme	scaffolds	912:920	arg1	adhesion					851:858	cell adhesion	846:858	cell adhesion	846:858	Cell viability, cell adhesion and cytotoxicity of Ca-SAPO-34/CS and Fe-Ca-SAPO-34 scaffolds were investigated by MTT assay and SEM on h-DPSCs which revealed cell proliferation no toxicity on scaffolds.
34314794	5	35	theme	scaffolds	912:920	arg1	cytotoxicity					864:875	cytotoxicity	864:875	cytotoxicity	864:875	Cell viability, cell adhesion and cytotoxicity of Ca-SAPO-34/CS and Fe-Ca-SAPO-34 scaffolds were investigated by MTT assay and SEM on h-DPSCs which revealed cell proliferation no toxicity on scaffolds.
34314794	8	36	theme	Alizarin	1358:1365	arg1	results					1405:1411	Alizarin red, Alkaline phosphatase and QRT-PCR results	1358:1411	Alizarin red, Alkaline phosphatase and QRT-PCR results	1358:1411	Alizarin red, Alkaline phosphatase and QRT-PCR results showed that Fe-Ca-loaded SAPO-34/CS can lead to osteoblast/odontoblast differentiation in DPSCs through the up-regulation of related genes, thus indicating that Fe-Ca-SAPO-34/CS has remarkable prospects as a biomaterial for hard tissue engineering.
34314794	4	37	theme	SAPO-34	622:628	arg1	effects					611:617	The effects	607:617	The effects of SAPO-34 and nanoparticles	607:646	The effects of SAPO-34 and nanoparticles were investigated by changes on the physicochemical properties of scaffolds including swelling ratio, density, porosity, bio-degradation, mechanical behavior, and biomineralization.
34314794	1	38	link	pulp-derived	257:268	arg1	cells					287:291	human dental pulp-derived mesenchymal stem cells	244:291	human dental pulp-derived mesenchymal stem cells	244:291	This research aimed to design innovative therapeutic bio-composites that enhance odontogenic and osteogenic differentiation of human dental pulp-derived mesenchymal stem cells (h-DPSCs) in-vitro regeneration.
34314794	4	39	theme	mechanical	786:795	arg1	behavior					797:804	mechanical behavior	786:804	mechanical behavior	786:804	The effects of SAPO-34 and nanoparticles were investigated by changes on the physicochemical properties of scaffolds including swelling ratio, density, porosity, bio-degradation, mechanical behavior, and biomineralization.
34314794	4	40	from	changes	669:675	arg1	properties					700:709	the physicochemical properties	680:709	the physicochemical properties of scaffolds including swelling ratio, density, porosity, bio-degradation, mechanical behavior, and biomineralization	680:827	The effects of SAPO-34 and nanoparticles were investigated by changes on the physicochemical properties of scaffolds including swelling ratio, density, porosity, bio-degradation, mechanical behavior, and biomineralization.
34314794	1	41	theme	osteogenic	214:223	arg1	differentiation					225:239	odontogenic and osteogenic differentiation	198:239	odontogenic and osteogenic differentiation of human dental pulp-derived mesenchymal stem cells	198:291	This research aimed to design innovative therapeutic bio-composites that enhance odontogenic and osteogenic differentiation of human dental pulp-derived mesenchymal stem cells (h-DPSCs) in-vitro regeneration.
34314794	7	42	theme	Fe2O3	1257:1261	arg1	incorporation					1240:1252	the incorporation	1236:1252	the incorporation of Fe2O3 to Ca-SAPO-34/CS scaffold	1236:1287	Moreover, the incorporation of Fe2O3 to Ca-SAPO-34/CS scaffold promoted the proliferation of hDPSCs and osteogenic differentiation.
34314794	8	43	theme	remarkable	1595:1604	arg1	prospects					1606:1614	remarkable prospects	1595:1614	remarkable prospects	1595:1614	Alizarin red, Alkaline phosphatase and QRT-PCR results showed that Fe-Ca-loaded SAPO-34/CS can lead to osteoblast/odontoblast differentiation in DPSCs through the up-regulation of related genes, thus indicating that Fe-Ca-SAPO-34/CS has remarkable prospects as a biomaterial for hard tissue engineering.
34314794	8	43	theme	remarkable	1595:1604	arg1	biomaterial					1621:1631	biomaterial	1621:1631	biomaterial	1621:1631	Alizarin red, Alkaline phosphatase and QRT-PCR results showed that Fe-Ca-loaded SAPO-34/CS can lead to osteoblast/odontoblast differentiation in DPSCs through the up-regulation of related genes, thus indicating that Fe-Ca-SAPO-34/CS has remarkable prospects as a biomaterial for hard tissue engineering.
34314794	3	44	theme	TGA	570:572	arg1	methods					598:604	ICP-AES, FT-IR, XRD, TGA, TEM, BET, SEM, and EDS methods	549:604	ICP-AES, FT-IR, XRD, TGA, TEM, BET, SEM, and EDS methods	549:604	The scaffolds and nanoparticles were characterized using ICP-AES, FT-IR, XRD, TGA, TEM, BET, SEM, and EDS methods.
34314794	3	45	theme	ICP-AES	549:555	arg1	methods					598:604	ICP-AES, FT-IR, XRD, TGA, TEM, BET, SEM, and EDS methods	549:604	ICP-AES, FT-IR, XRD, TGA, TEM, BET, SEM, and EDS methods	549:604	The scaffolds and nanoparticles were characterized using ICP-AES, FT-IR, XRD, TGA, TEM, BET, SEM, and EDS methods.
34314794	0	46	theme	scaffolds	45:53	arg1	fabrication					11:21	fabrication	11:21	fabrication	11:21	Design and fabrication of M-SAPO-34/chitosan scaffolds and evaluation of their effects on dental tissue engineering.
34314794	0	46	theme	scaffolds	45:53	arg1	Design					0:5	Design	0:5	Design	0:5	Design and fabrication of M-SAPO-34/chitosan scaffolds and evaluation of their effects on dental tissue engineering.
34314794	0	46	theme	scaffolds	45:53	arg1	evaluation					59:68	evaluation	59:68	evaluation of their effects on dental tissue engineering	59:114	Design and fabrication of M-SAPO-34/chitosan scaffolds and evaluation of their effects on dental tissue engineering.
34314794	3	47	theme	XRD	565:567	arg1	methods					598:604	ICP-AES, FT-IR, XRD, TGA, TEM, BET, SEM, and EDS methods	549:604	ICP-AES, FT-IR, XRD, TGA, TEM, BET, SEM, and EDS methods	549:604	The scaffolds and nanoparticles were characterized using ICP-AES, FT-IR, XRD, TGA, TEM, BET, SEM, and EDS methods.
34314794	0	48	from	effects	79:85	arg1	engineering					104:114	dental tissue engineering	90:114	dental tissue engineering	90:114	Design and fabrication of M-SAPO-34/chitosan scaffolds and evaluation of their effects on dental tissue engineering.
34314794	8	49	theme	related	1538:1544	arg1	genes					1546:1550	related genes	1538:1550	related genes	1538:1550	Alizarin red, Alkaline phosphatase and QRT-PCR results showed that Fe-Ca-loaded SAPO-34/CS can lead to osteoblast/odontoblast differentiation in DPSCs through the up-regulation of related genes, thus indicating that Fe-Ca-SAPO-34/CS has remarkable prospects as a biomaterial for hard tissue engineering.
34314794	2	50	theme	freeze-drying	467:479	arg1	technique					481:489	freeze-drying technique	467:489	freeze-drying technique	467:489	Herein, we report the fabrication of scaffolds containing chitosan, Ca-SAPO-34 monometallic and/or Fe-Ca-SAPO-34 bimetallic nanoparticles by freeze-drying technique.
34314794	0	51	theme	M-SAPO-34/chitosan	26:43	arg1	scaffolds					45:53	M-SAPO-34/chitosan scaffolds	26:53	M-SAPO-34/chitosan scaffolds	26:53	Design and fabrication of M-SAPO-34/chitosan scaffolds and evaluation of their effects on dental tissue engineering.
34314794	5	52	theme	Cell	830:833	arg1	viability					835:843	Cell viability	830:843	Cell viability	830:843	Cell viability, cell adhesion and cytotoxicity of Ca-SAPO-34/CS and Fe-Ca-SAPO-34 scaffolds were investigated by MTT assay and SEM on h-DPSCs which revealed cell proliferation no toxicity on scaffolds.
34314794	1	53	theme	human	244:248	arg1	cells					287:291	human dental pulp-derived mesenchymal stem cells	244:291	human dental pulp-derived mesenchymal stem cells	244:291	This research aimed to design innovative therapeutic bio-composites that enhance odontogenic and osteogenic differentiation of human dental pulp-derived mesenchymal stem cells (h-DPSCs) in-vitro regeneration.
34314794	5	54	from	assay	947:951	arg1	h-DPSCs					964:970	h-DPSCs	964:970	h-DPSCs which revealed cell proliferation no toxicity on scaffolds	964:1029	Cell viability, cell adhesion and cytotoxicity of Ca-SAPO-34/CS and Fe-Ca-SAPO-34 scaffolds were investigated by MTT assay and SEM on h-DPSCs which revealed cell proliferation no toxicity on scaffolds.
34314794	5	55	theme	MTT	943:945	arg1	assay					947:951	MTT assay	943:951	MTT assay	943:951	Cell viability, cell adhesion and cytotoxicity of Ca-SAPO-34/CS and Fe-Ca-SAPO-34 scaffolds were investigated by MTT assay and SEM on h-DPSCs which revealed cell proliferation no toxicity on scaffolds.
34314794	6	56	theme	positive	1104:1111	arg1	effect					1113:1118	a positive effect	1102:1118	a positive effect on differentiation of hDPSCs into osteogenic/odontogenic cells	1102:1181	Cell tests demonstrated that Ca-SAPO-34/CS scaffold clearly displayed a positive effect on differentiation of hDPSCs into osteogenic/odontogenic cells and moderate effect on cell proliferation.
34314794	8	57	theme	tissue	1642:1647	arg1	engineering					1649:1659	hard tissue engineering	1637:1659	hard tissue engineering	1637:1659	Alizarin red, Alkaline phosphatase and QRT-PCR results showed that Fe-Ca-loaded SAPO-34/CS can lead to osteoblast/odontoblast differentiation in DPSCs through the up-regulation of related genes, thus indicating that Fe-Ca-SAPO-34/CS has remarkable prospects as a biomaterial for hard tissue engineering.
34314794	8	58	theme	Fe-Ca-loaded	1425:1436	arg1	SAPO-34/CS					1438:1447	Fe-Ca-loaded SAPO-34/CS	1425:1447	Fe-Ca-loaded SAPO-34/CS	1425:1447	Alizarin red, Alkaline phosphatase and QRT-PCR results showed that Fe-Ca-loaded SAPO-34/CS can lead to osteoblast/odontoblast differentiation in DPSCs through the up-regulation of related genes, thus indicating that Fe-Ca-SAPO-34/CS has remarkable prospects as a biomaterial for hard tissue engineering.
34314794	2	59	contain	containing	373:382	arg1	scaffolds					363:371	scaffolds	363:371	scaffolds containing chitosan, Ca-SAPO-34 monometallic and/or Fe-Ca-SAPO-34 bimetallic nanoparticles by freeze-drying technique	363:489	Herein, we report the fabrication of scaffolds containing chitosan, Ca-SAPO-34 monometallic and/or Fe-Ca-SAPO-34 bimetallic nanoparticles by freeze-drying technique.
34314794	2	59	contain	containing	373:382	arg2	nanoparticles					450:462	chitosan, Ca-SAPO-34 monometallic and/or Fe-Ca-SAPO-34 bimetallic nanoparticles	384:462	chitosan, Ca-SAPO-34 monometallic and/or Fe-Ca-SAPO-34 bimetallic nanoparticles by freeze-drying technique	384:489	Herein, we report the fabrication of scaffolds containing chitosan, Ca-SAPO-34 monometallic and/or Fe-Ca-SAPO-34 bimetallic nanoparticles by freeze-drying technique.
34314794	1	60	dep	enhance	190:196	arg1	h-DPSCs					294:300	h-DPSCs	294:300	h-DPSCs	294:300	This research aimed to design innovative therapeutic bio-composites that enhance odontogenic and osteogenic differentiation of human dental pulp-derived mesenchymal stem cells (h-DPSCs) in-vitro regeneration.
34314794	2	61	theme	scaffolds	363:371	arg1	fabrication					348:358	the fabrication	344:358	the fabrication of scaffolds containing chitosan, Ca-SAPO-34 monometallic and/or Fe-Ca-SAPO-34 bimetallic nanoparticles by freeze-drying technique	344:489	Herein, we report the fabrication of scaffolds containing chitosan, Ca-SAPO-34 monometallic and/or Fe-Ca-SAPO-34 bimetallic nanoparticles by freeze-drying technique.
34314794	0	62	from	engineering	104:114	arg1	fabrication					11:21	fabrication	11:21	fabrication	11:21	Design and fabrication of M-SAPO-34/chitosan scaffolds and evaluation of their effects on dental tissue engineering.
34314794	0	62	from	engineering	104:114	arg1	Design					0:5	Design	0:5	Design	0:5	Design and fabrication of M-SAPO-34/chitosan scaffolds and evaluation of their effects on dental tissue engineering.
34314794	0	62	from	engineering	104:114	arg1	evaluation					59:68	evaluation	59:68	evaluation of their effects on dental tissue engineering	59:114	Design and fabrication of M-SAPO-34/chitosan scaffolds and evaluation of their effects on dental tissue engineering.
34314794	4	63	theme	scaffolds	714:722	arg1	properties					700:709	the physicochemical properties	680:709	the physicochemical properties of scaffolds including swelling ratio, density, porosity, bio-degradation, mechanical behavior, and biomineralization	680:827	The effects of SAPO-34 and nanoparticles were investigated by changes on the physicochemical properties of scaffolds including swelling ratio, density, porosity, bio-degradation, mechanical behavior, and biomineralization.
34314794	6	64	theme	cell	1206:1209	arg1	proliferation					1211:1223	cell proliferation	1206:1223	cell proliferation	1206:1223	Cell tests demonstrated that Ca-SAPO-34/CS scaffold clearly displayed a positive effect on differentiation of hDPSCs into osteogenic/odontogenic cells and moderate effect on cell proliferation.
34314794	8	65	from	differentiation	1484:1498	arg1	DPSCs					1503:1507	DPSCs	1503:1507	DPSCs	1503:1507	Alizarin red, Alkaline phosphatase and QRT-PCR results showed that Fe-Ca-loaded SAPO-34/CS can lead to osteoblast/odontoblast differentiation in DPSCs through the up-regulation of related genes, thus indicating that Fe-Ca-SAPO-34/CS has remarkable prospects as a biomaterial for hard tissue engineering.
34314794	1	66	theme	dental	250:255	arg1	cells					287:291	human dental pulp-derived mesenchymal stem cells	244:291	human dental pulp-derived mesenchymal stem cells	244:291	This research aimed to design innovative therapeutic bio-composites that enhance odontogenic and osteogenic differentiation of human dental pulp-derived mesenchymal stem cells (h-DPSCs) in-vitro regeneration.
34314794	8	67	theme	hard	1637:1640	arg1	engineering					1649:1659	hard tissue engineering	1637:1659	hard tissue engineering	1637:1659	Alizarin red, Alkaline phosphatase and QRT-PCR results showed that Fe-Ca-loaded SAPO-34/CS can lead to osteoblast/odontoblast differentiation in DPSCs through the up-regulation of related genes, thus indicating that Fe-Ca-SAPO-34/CS has remarkable prospects as a biomaterial for hard tissue engineering.
34314794	2	68	theme	Ca-SAPO-34	394:403	arg1	monometallic					405:416	Ca-SAPO-34 monometallic	394:416	Ca-SAPO-34 monometallic	394:416	Herein, we report the fabrication of scaffolds containing chitosan, Ca-SAPO-34 monometallic and/or Fe-Ca-SAPO-34 bimetallic nanoparticles by freeze-drying technique.
34314794	1	69	theme	pulp-derived	257:268	arg1	cells					287:291	human dental pulp-derived mesenchymal stem cells	244:291	human dental pulp-derived mesenchymal stem cells	244:291	This research aimed to design innovative therapeutic bio-composites that enhance odontogenic and osteogenic differentiation of human dental pulp-derived mesenchymal stem cells (h-DPSCs) in-vitro regeneration.
34314794	5	70	theme	Fe-Ca-SAPO-34	898:910	arg1	scaffolds					912:920	Ca-SAPO-34/CS and Fe-Ca-SAPO-34 scaffolds	880:920	Ca-SAPO-34/CS and Fe-Ca-SAPO-34 scaffolds	880:920	Cell viability, cell adhesion and cytotoxicity of Ca-SAPO-34/CS and Fe-Ca-SAPO-34 scaffolds were investigated by MTT assay and SEM on h-DPSCs which revealed cell proliferation no toxicity on scaffolds.
34314794	8	71	theme	phosphatase	1381:1391	arg1	results					1405:1411	Alizarin red, Alkaline phosphatase and QRT-PCR results	1358:1411	Alizarin red, Alkaline phosphatase and QRT-PCR results	1358:1411	Alizarin red, Alkaline phosphatase and QRT-PCR results showed that Fe-Ca-loaded SAPO-34/CS can lead to osteoblast/odontoblast differentiation in DPSCs through the up-regulation of related genes, thus indicating that Fe-Ca-SAPO-34/CS has remarkable prospects as a biomaterial for hard tissue engineering.
34314794	8	72	theme	Alkaline	1372:1379	arg1	phosphatase					1381:1391	Alkaline phosphatase	1372:1391	Alkaline phosphatase	1372:1391	Alizarin red, Alkaline phosphatase and QRT-PCR results showed that Fe-Ca-loaded SAPO-34/CS can lead to osteoblast/odontoblast differentiation in DPSCs through the up-regulation of related genes, thus indicating that Fe-Ca-SAPO-34/CS has remarkable prospects as a biomaterial for hard tissue engineering.
34314794	2	73	theme	monometallic	405:416	arg1	nanoparticles					450:462	chitosan, Ca-SAPO-34 monometallic and/or Fe-Ca-SAPO-34 bimetallic nanoparticles	384:462	chitosan, Ca-SAPO-34 monometallic and/or Fe-Ca-SAPO-34 bimetallic nanoparticles by freeze-drying technique	384:489	Herein, we report the fabrication of scaffolds containing chitosan, Ca-SAPO-34 monometallic and/or Fe-Ca-SAPO-34 bimetallic nanoparticles by freeze-drying technique.
34314794	1	74	theme	mesenchymal	270:280	arg1	cells					287:291	human dental pulp-derived mesenchymal stem cells	244:291	human dental pulp-derived mesenchymal stem cells	244:291	This research aimed to design innovative therapeutic bio-composites that enhance odontogenic and osteogenic differentiation of human dental pulp-derived mesenchymal stem cells (h-DPSCs) in-vitro regeneration.
34314794	7	75	theme	osteogenic	1330:1339	arg1	differentiation					1341:1355	osteogenic differentiation	1330:1355	osteogenic differentiation	1330:1355	Moreover, the incorporation of Fe2O3 to Ca-SAPO-34/CS scaffold promoted the proliferation of hDPSCs and osteogenic differentiation.
34314794	4	76	theme	nanoparticles	634:646	arg1	effects					611:617	The effects	607:617	The effects of SAPO-34 and nanoparticles	607:646	The effects of SAPO-34 and nanoparticles were investigated by changes on the physicochemical properties of scaffolds including swelling ratio, density, porosity, bio-degradation, mechanical behavior, and biomineralization.
34314794	6	77	from	effect	1196:1201	arg1	differentiation					1123:1137	differentiation	1123:1137	differentiation of hDPSCs into osteogenic/odontogenic cells	1123:1181	Cell tests demonstrated that Ca-SAPO-34/CS scaffold clearly displayed a positive effect on differentiation of hDPSCs into osteogenic/odontogenic cells and moderate effect on cell proliferation.
34314794	6	77	from	effect	1196:1201	arg1	proliferation					1211:1223	cell proliferation	1206:1223	cell proliferation	1206:1223	Cell tests demonstrated that Ca-SAPO-34/CS scaffold clearly displayed a positive effect on differentiation of hDPSCs into osteogenic/odontogenic cells and moderate effect on cell proliferation.
34314794	1	78	theme	innovative	147:156	arg1	bio-composites					170:183	innovative therapeutic bio-composites	147:183	innovative therapeutic bio-composites that enhance odontogenic and osteogenic differentiation of human dental pulp-derived mesenchymal stem cells (h-DPSCs) in-vitro regeneration	147:323	This research aimed to design innovative therapeutic bio-composites that enhance odontogenic and osteogenic differentiation of human dental pulp-derived mesenchymal stem cells (h-DPSCs) in-vitro regeneration.
34314794	8	79	theme	QRT-PCR	1397:1403	arg1	results					1405:1411	Alizarin red, Alkaline phosphatase and QRT-PCR results	1358:1411	Alizarin red, Alkaline phosphatase and QRT-PCR results	1358:1411	Alizarin red, Alkaline phosphatase and QRT-PCR results showed that Fe-Ca-loaded SAPO-34/CS can lead to osteoblast/odontoblast differentiation in DPSCs through the up-regulation of related genes, thus indicating that Fe-Ca-SAPO-34/CS has remarkable prospects as a biomaterial for hard tissue engineering.
34314794	1	80	theme	stem	282:285	arg1	cells					287:291	human dental pulp-derived mesenchymal stem cells	244:291	human dental pulp-derived mesenchymal stem cells	244:291	This research aimed to design innovative therapeutic bio-composites that enhance odontogenic and osteogenic differentiation of human dental pulp-derived mesenchymal stem cells (h-DPSCs) in-vitro regeneration.
33227032	1	0	from	effects	155:161	arg1	traits					239:244	carcass traits	231:244	carcass traits	231:244	The objective of this study was to evaluate the effects of the association of cottonseed processing method with chitosan on carcass traits and meat quality of lambs finished in feedlot.
33227032	1	0	from	effects	155:161	arg1	quality					255:261	meat quality	250:261	meat quality of lambs finished in feedlot	250:290	The objective of this study was to evaluate the effects of the association of cottonseed processing method with chitosan on carcass traits and meat quality of lambs finished in feedlot.
33227032	7	1	theme	feedlot	1180:1186	arg1	lambs					1188:1192	feedlot lambs	1180:1192	feedlot lambs	1180:1192	Ground cottonseed associated with chitosan increases the concentration of unsaturated fatty acids in the meat of feedlot lambs.
33227032	1	2	theme	carcass	231:237	arg1	traits					239:244	carcass traits	231:244	carcass traits	231:244	The objective of this study was to evaluate the effects of the association of cottonseed processing method with chitosan on carcass traits and meat quality of lambs finished in feedlot.
33227032	0	3	theme	meat	76:79	arg1	quality					81:87	meat quality	76:87	meat quality of feedlot lambs	76:104	Chitosan and cottonseed processing method association on carcass traits and meat quality of feedlot lambs.
33227032	2	4	theme	body	322:325	arg1	weight					327:332	an average body weight	311:332	an average body weight of 20.6 kg	311:343	Eighty lambs with an average body weight of 20.6 kg, with 04 months of age, were distributed in a completely randomized design, in a 2 x 2 factorial arrangement.
33227032	5	5	theme	lamb	897:900	arg1	diets					902:906	the lamb diets	893:906	the lamb diets	893:906	The pH, color, cooking losses, shear force, and proximate composition of meat were also not affected (P>0.05) by the processing method of cottonseed or its association with chitosan in the lamb diets.
33227032	0	6	theme	feedlot	92:98	arg1	lambs					100:104	feedlot lambs	92:104	feedlot lambs	92:104	Chitosan and cottonseed processing method association on carcass traits and meat quality of feedlot lambs.
33227032	4	7	with	association	591:601	arg1	chitosan					640:647	chitosan	640:647	chitosan in the lamb diet	640:664	The association of cottonseed processing method with chitosan in the lamb diet did not affect (P>0.05) carcasses traits.
33227032	4	8	theme	lamb	656:659	arg1	diet					661:664	the lamb diet	652:664	the lamb diet	652:664	The association of cottonseed processing method with chitosan in the lamb diet did not affect (P>0.05) carcasses traits.
33227032	6	9	from	increase	922:929	arg1	acids					1008:1012	palmitoleic (c9-C16:1; P = 0.01) and conjugated linoleic (P = 0.02) fatty acids	934:1012	palmitoleic (c9-C16:1; P = 0.01) and conjugated linoleic (P = 0.02) fatty acids	934:1012	There was an increase in palmitoleic (c9-C16:1; P = 0.01) and conjugated linoleic (P = 0.02) fatty acids when ground cottonseed was associated with chitosan.
33227032	3	10	theme	cottonseed	491:500	arg1	method					513:518	two cottonseed processing method	487:518	two cottonseed processing method (whole or ground)	487:536	The factors were represented by two cottonseed processing method (whole or ground) and two levels of chitosan (0 and 136 mg/kg BW).
33227032	6	11	theme	linoleic	982:989	arg1	acids					1008:1012	palmitoleic (c9-C16:1; P = 0.01) and conjugated linoleic (P = 0.02) fatty acids	934:1012	palmitoleic (c9-C16:1; P = 0.01) and conjugated linoleic (P = 0.02) fatty acids	934:1012	There was an increase in palmitoleic (c9-C16:1; P = 0.01) and conjugated linoleic (P = 0.02) fatty acids when ground cottonseed was associated with chitosan.
33227032	5	12	from	method	836:841	arg1	diets					902:906	the lamb diets	893:906	the lamb diets	893:906	The pH, color, cooking losses, shear force, and proximate composition of meat were also not affected (P>0.05) by the processing method of cottonseed or its association with chitosan in the lamb diets.
33227032	1	13	theme	meat	250:253	arg1	quality					255:261	meat quality	250:261	meat quality of lambs finished in feedlot	250:290	The objective of this study was to evaluate the effects of the association of cottonseed processing method with chitosan on carcass traits and meat quality of lambs finished in feedlot.
33227032	5	14	theme	proximate	756:764	arg1	composition					766:776	proximate composition	756:776	proximate composition	756:776	The pH, color, cooking losses, shear force, and proximate composition of meat were also not affected (P>0.05) by the processing method of cottonseed or its association with chitosan in the lamb diets.
33227032	6	15	theme	conjugated	971:980	arg1	acids					1008:1012	palmitoleic (c9-C16:1; P = 0.01) and conjugated linoleic (P = 0.02) fatty acids	934:1012	palmitoleic (c9-C16:1; P = 0.01) and conjugated linoleic (P = 0.02) fatty acids	934:1012	There was an increase in palmitoleic (c9-C16:1; P = 0.01) and conjugated linoleic (P = 0.02) fatty acids when ground cottonseed was associated with chitosan.
33227032	7	16	theme	fatty	1153:1157	arg1	acids					1159:1163	unsaturated fatty acids	1141:1163	unsaturated fatty acids	1141:1163	Ground cottonseed associated with chitosan increases the concentration of unsaturated fatty acids in the meat of feedlot lambs.
33227032	2	17	theme	04	351:352	arg1	months					354:359	04 months	351:359	04 months of age	351:366	Eighty lambs with an average body weight of 20.6 kg, with 04 months of age, were distributed in a completely randomized design, in a 2 x 2 factorial arrangement.
33227032	0	18	theme	lambs	100:104	arg1	traits					65:70	carcass traits	57:70	carcass traits	57:70	Chitosan and cottonseed processing method association on carcass traits and meat quality of feedlot lambs.
33227032	0	18	theme	lambs	100:104	arg1	quality					81:87	meat quality	76:87	meat quality of feedlot lambs	76:104	Chitosan and cottonseed processing method association on carcass traits and meat quality of feedlot lambs.
33227032	0	19	from	association	42:52	arg1	traits					65:70	carcass traits	57:70	carcass traits	57:70	Chitosan and cottonseed processing method association on carcass traits and meat quality of feedlot lambs.
33227032	0	19	from	association	42:52	arg1	quality					81:87	meat quality	76:87	meat quality of feedlot lambs	76:104	Chitosan and cottonseed processing method association on carcass traits and meat quality of feedlot lambs.
33227032	5	20	theme	cooking	723:729	arg1	losses					731:736	cooking losses	723:736	cooking losses	723:736	The pH, color, cooking losses, shear force, and proximate composition of meat were also not affected (P>0.05) by the processing method of cottonseed or its association with chitosan in the lamb diets.
33227032	4	21	from	chitosan	640:647	arg1	diet					661:664	the lamb diet	652:664	the lamb diet	652:664	The association of cottonseed processing method with chitosan in the lamb diet did not affect (P>0.05) carcasses traits.
33227032	2	22	theme	Eighty	293:298	arg1	lambs					300:304	Eighty lambs	293:304	Eighty lambs	293:304	Eighty lambs with an average body weight of 20.6 kg, with 04 months of age, were distributed in a completely randomized design, in a 2 x 2 factorial arrangement.
33227032	3	23	theme	processing	502:511	arg1	method					513:518	two cottonseed processing method	487:518	two cottonseed processing method (whole or ground)	487:536	The factors were represented by two cottonseed processing method (whole or ground) and two levels of chitosan (0 and 136 mg/kg BW).
33227032	2	24	theme	kg	342:343	arg1	weight					327:332	an average body weight	311:332	an average body weight of 20.6 kg	311:343	Eighty lambs with an average body weight of 20.6 kg, with 04 months of age, were distributed in a completely randomized design, in a 2 x 2 factorial arrangement.
33227032	0	25	theme	processing	24:33	arg1	method					35:40	cottonseed processing method	13:40	cottonseed processing method	13:40	Chitosan and cottonseed processing method association on carcass traits and meat quality of feedlot lambs.
33227032	4	26	theme	method	628:633	arg1	association					591:601	The association	587:601	The association of cottonseed processing method with chitosan in the lamb diet	587:664	The association of cottonseed processing method with chitosan in the lamb diet did not affect (P>0.05) carcasses traits.
33227032	2	27	dep	factorial	432:440	arg1	2					426:426	2	426:426	2	426:426	Eighty lambs with an average body weight of 20.6 kg, with 04 months of age, were distributed in a completely randomized design, in a 2 x 2 factorial arrangement.
33227032	2	27	dep	factorial	432:440	arg1	2					430:430	2	430:430	2	430:430	Eighty lambs with an average body weight of 20.6 kg, with 04 months of age, were distributed in a completely randomized design, in a 2 x 2 factorial arrangement.
33227032	5	28	theme	meat	781:784	arg1	composition					766:776	proximate composition	756:776	proximate composition	756:776	The pH, color, cooking losses, shear force, and proximate composition of meat were also not affected (P>0.05) by the processing method of cottonseed or its association with chitosan in the lamb diets.
33227032	5	28	theme	meat	781:784	arg1	color					716:720	color	716:720	color	716:720	The pH, color, cooking losses, shear force, and proximate composition of meat were also not affected (P>0.05) by the processing method of cottonseed or its association with chitosan in the lamb diets.
33227032	5	28	theme	meat	781:784	arg1	losses					731:736	cooking losses	723:736	cooking losses	723:736	The pH, color, cooking losses, shear force, and proximate composition of meat were also not affected (P>0.05) by the processing method of cottonseed or its association with chitosan in the lamb diets.
33227032	5	28	theme	meat	781:784	arg1	pH					712:713	pH	712:713	pH	712:713	The pH, color, cooking losses, shear force, and proximate composition of meat were also not affected (P>0.05) by the processing method of cottonseed or its association with chitosan in the lamb diets.
33227032	5	28	theme	meat	781:784	arg1	force					745:749	shear force	739:749	shear force	739:749	The pH, color, cooking losses, shear force, and proximate composition of meat were also not affected (P>0.05) by the processing method of cottonseed or its association with chitosan in the lamb diets.
33227032	5	29	theme	cottonseed	846:855	arg1	method					836:841	the processing method	821:841	the processing method of cottonseed	821:855	The pH, color, cooking losses, shear force, and proximate composition of meat were also not affected (P>0.05) by the processing method of cottonseed or its association with chitosan in the lamb diets.
33227032	5	29	theme	cottonseed	846:855	arg1	association					864:874	its association	860:874	its association with chitosan in the lamb diets	860:906	The pH, color, cooking losses, shear force, and proximate composition of meat were also not affected (P>0.05) by the processing method of cottonseed or its association with chitosan in the lamb diets.
33227032	1	30	theme	association	170:180	arg1	effects					155:161	the effects	151:161	the effects of the association of cottonseed processing method with chitosan on carcass traits and meat quality of lambs finished in feedlot	151:290	The objective of this study was to evaluate the effects of the association of cottonseed processing method with chitosan on carcass traits and meat quality of lambs finished in feedlot.
33227032	0	31	theme	cottonseed	13:22	arg1	method					35:40	cottonseed processing method	13:40	cottonseed processing method	13:40	Chitosan and cottonseed processing method association on carcass traits and meat quality of feedlot lambs.
33227032	1	32	theme	lambs	266:270	arg1	traits					239:244	carcass traits	231:244	carcass traits	231:244	The objective of this study was to evaluate the effects of the association of cottonseed processing method with chitosan on carcass traits and meat quality of lambs finished in feedlot.
33227032	1	32	theme	lambs	266:270	arg1	quality					255:261	meat quality	250:261	meat quality of lambs finished in feedlot	250:290	The objective of this study was to evaluate the effects of the association of cottonseed processing method with chitosan on carcass traits and meat quality of lambs finished in feedlot.
33227032	3	33	theme	chitosan	556:563	arg1	method					513:518	two cottonseed processing method	487:518	two cottonseed processing method (whole or ground)	487:536	The factors were represented by two cottonseed processing method (whole or ground) and two levels of chitosan (0 and 136 mg/kg BW).
33227032	3	33	theme	chitosan	556:563	arg1	levels					546:551	two levels	542:551	two levels of chitosan (0 and 136 mg/kg BW)	542:584	The factors were represented by two cottonseed processing method (whole or ground) and two levels of chitosan (0 and 136 mg/kg BW).
33227032	2	34	theme	age	364:366	arg1	months					354:359	04 months	351:359	04 months of age	351:366	Eighty lambs with an average body weight of 20.6 kg, with 04 months of age, were distributed in a completely randomized design, in a 2 x 2 factorial arrangement.
33227032	1	35	with	association	170:180	arg1	chitosan					219:226	chitosan	219:226	chitosan	219:226	The objective of this study was to evaluate the effects of the association of cottonseed processing method with chitosan on carcass traits and meat quality of lambs finished in feedlot.
33227032	2	36	with	lambs	300:304	arg1	weight					327:332	an average body weight	311:332	an average body weight of 20.6 kg	311:343	Eighty lambs with an average body weight of 20.6 kg, with 04 months of age, were distributed in a completely randomized design, in a 2 x 2 factorial arrangement.
33227032	2	36	with	lambs	300:304	arg1	months					354:359	04 months	351:359	04 months of age	351:366	Eighty lambs with an average body weight of 20.6 kg, with 04 months of age, were distributed in a completely randomized design, in a 2 x 2 factorial arrangement.
33227032	4	37	theme	carcasses	690:698	arg1	traits					700:705	(P>0.05) carcasses traits	681:705	(P>0.05) carcasses traits	681:705	The association of cottonseed processing method with chitosan in the lamb diet did not affect (P>0.05) carcasses traits.
33227032	5	38	from	association	864:874	arg1	diets					902:906	the lamb diets	893:906	the lamb diets	893:906	The pH, color, cooking losses, shear force, and proximate composition of meat were also not affected (P>0.05) by the processing method of cottonseed or its association with chitosan in the lamb diets.
33227032	1	39	theme	cottonseed	185:194	arg1	method					207:212	cottonseed processing method	185:212	cottonseed processing method	185:212	The objective of this study was to evaluate the effects of the association of cottonseed processing method with chitosan on carcass traits and meat quality of lambs finished in feedlot.
33227032	3	40	dep	method	513:518	arg1	whole					521:525	whole	521:525	whole	521:525	The factors were represented by two cottonseed processing method (whole or ground) and two levels of chitosan (0 and 136 mg/kg BW).
33227032	3	40	dep	method	513:518	arg1	ground					530:535	ground	530:535	ground	530:535	The factors were represented by two cottonseed processing method (whole or ground) and two levels of chitosan (0 and 136 mg/kg BW).
33227032	2	41	dep	arrangement	442:452	arg1	factorial					432:440	factorial	432:440	factorial	432:440	Eighty lambs with an average body weight of 20.6 kg, with 04 months of age, were distributed in a completely randomized design, in a 2 x 2 factorial arrangement.
33227032	6	42	dep	palmitoleic	934:944	arg1	=					959:959	=	959:959	=	959:959	There was an increase in palmitoleic (c9-C16:1; P = 0.01) and conjugated linoleic (P = 0.02) fatty acids when ground cottonseed was associated with chitosan.
33227032	1	43	theme	processing	196:205	arg1	method					207:212	cottonseed processing method	185:212	cottonseed processing method	185:212	The objective of this study was to evaluate the effects of the association of cottonseed processing method with chitosan on carcass traits and meat quality of lambs finished in feedlot.
33227032	0	44	theme	carcass	57:63	arg1	traits					65:70	carcass traits	57:70	carcass traits	57:70	Chitosan and cottonseed processing method association on carcass traits and meat quality of feedlot lambs.
33227032	5	45	theme	shear	739:743	arg1	force					745:749	shear force	739:749	shear force	739:749	The pH, color, cooking losses, shear force, and proximate composition of meat were also not affected (P>0.05) by the processing method of cottonseed or its association with chitosan in the lamb diets.
33227032	2	46	theme	average	314:320	arg1	weight					327:332	an average body weight	311:332	an average body weight of 20.6 kg	311:343	Eighty lambs with an average body weight of 20.6 kg, with 04 months of age, were distributed in a completely randomized design, in a 2 x 2 factorial arrangement.
33227032	6	47	dep	=	959:959	arg1	c9-C16:1					947:954	c9-C16:1	947:954	c9-C16:1	947:954	There was an increase in palmitoleic (c9-C16:1; P = 0.01) and conjugated linoleic (P = 0.02) fatty acids when ground cottonseed was associated with chitosan.
33227032	6	48	theme	fatty	1002:1006	arg1	acids					1008:1012	palmitoleic (c9-C16:1; P = 0.01) and conjugated linoleic (P = 0.02) fatty acids	934:1012	palmitoleic (c9-C16:1; P = 0.01) and conjugated linoleic (P = 0.02) fatty acids	934:1012	There was an increase in palmitoleic (c9-C16:1; P = 0.01) and conjugated linoleic (P = 0.02) fatty acids when ground cottonseed was associated with chitosan.
33227032	7	49	theme	acids	1159:1163	arg1	concentration					1124:1136	the concentration	1120:1136	the concentration of unsaturated fatty acids in the meat of feedlot lambs	1120:1192	Ground cottonseed associated with chitosan increases the concentration of unsaturated fatty acids in the meat of feedlot lambs.
33227032	3	50	theme	mg/kg	576:580	arg1	chitosan					556:563	chitosan	556:563	chitosan (0 and 136 mg/kg BW)	556:584	The factors were represented by two cottonseed processing method (whole or ground) and two levels of chitosan (0 and 136 mg/kg BW).
33227032	3	50	theme	mg/kg	576:580	arg1	BW					582:583	0 and 136 mg/kg BW	566:583	0 and 136 mg/kg BW	566:583	The factors were represented by two cottonseed processing method (whole or ground) and two levels of chitosan (0 and 136 mg/kg BW).
33227032	7	51	theme	lambs	1188:1192	arg1	meat					1172:1175	the meat	1168:1175	the meat of feedlot lambs	1168:1192	Ground cottonseed associated with chitosan increases the concentration of unsaturated fatty acids in the meat of feedlot lambs.
33227032	4	52	theme	processing	617:626	arg1	method					628:633	cottonseed processing method	606:633	cottonseed processing method	606:633	The association of cottonseed processing method with chitosan in the lamb diet did not affect (P>0.05) carcasses traits.
33227032	6	53	theme	palmitoleic	934:944	arg1	acids					1008:1012	palmitoleic (c9-C16:1; P = 0.01) and conjugated linoleic (P = 0.02) fatty acids	934:1012	palmitoleic (c9-C16:1; P = 0.01) and conjugated linoleic (P = 0.02) fatty acids	934:1012	There was an increase in palmitoleic (c9-C16:1; P = 0.01) and conjugated linoleic (P = 0.02) fatty acids when ground cottonseed was associated with chitosan.
33227032	1	54	theme	method	207:212	arg1	association					170:180	the association	166:180	the association of cottonseed processing method with chitosan	166:226	The objective of this study was to evaluate the effects of the association of cottonseed processing method with chitosan on carcass traits and meat quality of lambs finished in feedlot.
33227032	5	55	theme	processing	825:834	arg1	method					836:841	the processing method	821:841	the processing method of cottonseed	821:855	The pH, color, cooking losses, shear force, and proximate composition of meat were also not affected (P>0.05) by the processing method of cottonseed or its association with chitosan in the lamb diets.
33227032	4	56	theme	cottonseed	606:615	arg1	method					628:633	cottonseed processing method	606:633	cottonseed processing method	606:633	The association of cottonseed processing method with chitosan in the lamb diet did not affect (P>0.05) carcasses traits.
33227032	5	57	with	method	836:841	arg1	chitosan					881:888	chitosan	881:888	chitosan	881:888	The pH, color, cooking losses, shear force, and proximate composition of meat were also not affected (P>0.05) by the processing method of cottonseed or its association with chitosan in the lamb diets.
33227032	6	58	dep	linoleic	982:989	arg1	P					992:992	P = 0.02	992:999	P = 0.02	992:999	There was an increase in palmitoleic (c9-C16:1; P = 0.01) and conjugated linoleic (P = 0.02) fatty acids when ground cottonseed was associated with chitosan.
33227032	6	59	theme	=	994:994	arg1	P					992:992	P = 0.02	992:999	P = 0.02	992:999	There was an increase in palmitoleic (c9-C16:1; P = 0.01) and conjugated linoleic (P = 0.02) fatty acids when ground cottonseed was associated with chitosan.
33227032	7	60	from	concentration	1124:1136	arg1	meat					1172:1175	the meat	1168:1175	the meat of feedlot lambs	1168:1192	Ground cottonseed associated with chitosan increases the concentration of unsaturated fatty acids in the meat of feedlot lambs.
33227032	1	61	theme	study	129:133	arg1	objective					111:119	The objective	107:119	The objective of this study	107:133	The objective of this study was to evaluate the effects of the association of cottonseed processing method with chitosan on carcass traits and meat quality of lambs finished in feedlot.
33227032	7	62	theme	unsaturated	1141:1151	arg1	acids					1159:1163	unsaturated fatty acids	1141:1163	unsaturated fatty acids	1141:1163	Ground cottonseed associated with chitosan increases the concentration of unsaturated fatty acids in the meat of feedlot lambs.
33227032	2	63	theme	randomized	402:411	arg1	design					413:418	a completely randomized design	389:418	a completely randomized design	389:418	Eighty lambs with an average body weight of 20.6 kg, with 04 months of age, were distributed in a completely randomized design, in a 2 x 2 factorial arrangement.
33227032	5	64	dep	pH	712:713	arg1	The					708:710	The	708:710	The	708:710	The pH, color, cooking losses, shear force, and proximate composition of meat were also not affected (P>0.05) by the processing method of cottonseed or its association with chitosan in the lamb diets.
33227032	5	65	dep	affected	800:807	arg1	P>0.05					810:815	P>0.05	810:815	P>0.05	810:815	The pH, color, cooking losses, shear force, and proximate composition of meat were also not affected (P>0.05) by the processing method of cottonseed or its association with chitosan in the lamb diets.
33227032	5	66	with	association	864:874	arg1	chitosan					881:888	chitosan	881:888	chitosan	881:888	The pH, color, cooking losses, shear force, and proximate composition of meat were also not affected (P>0.05) by the processing method of cottonseed or its association with chitosan in the lamb diets.
32219929	0	0	theme	milk	51:54	arg1	production					56:65	milk production	51:65	milk production	51:65	Effects of white, yellow, and blue colored LEDs on milk production, milk composition, and physiological responses in dairy cattle.
32219929	10	1	theme	acid	1168:1171	arg1	levels					1173:1178	Nonesterified fatty acid levels	1148:1178	Nonesterified fatty acid levels in the yellow and blue LED groups	1148:1212	Nonesterified fatty acid levels in the yellow and blue LED groups decreased to the greatest extent compared to the start point.
32219929	8	2	theme	Prolactin	986:994	arg1	concentrations					996:1009	Prolactin concentrations	986:1009	Prolactin concentrations	986:1009	Prolactin concentrations were higher in the white and blue LED groups than in the control.
32219929	9	3	theme	LED	1120:1122	arg1	group					1124:1128	the blue LED group	1111:1128	the blue LED group	1111:1128	Cortisol level was the highest in the blue LED group among the groups.
32219929	5	4	theme	milk	636:639	arg1	production					641:650	milk production	636:650	milk production	636:650	Yellow and blue LED groups demonstrated greater rates of decline in milk production than control and white LED groups.
32219929	7	5	theme	insulin-like	949:960	arg1	factor-1					969:976	insulin-like growth factor-1	949:976	insulin-like growth factor-1	949:976	Extended exposure to blue LED light lowered antioxidant enzyme activity and insulin-like growth factor-1 levels.
32219929	10	6	theme	LED	1203:1205	arg1	groups					1207:1212	the yellow and blue LED groups	1183:1212	the yellow and blue LED groups	1183:1212	Nonesterified fatty acid levels in the yellow and blue LED groups decreased to the greatest extent compared to the start point.
32219929	7	7	theme	growth	962:967	arg1	factor-1					969:976	insulin-like growth factor-1	949:976	insulin-like growth factor-1	949:976	Extended exposure to blue LED light lowered antioxidant enzyme activity and insulin-like growth factor-1 levels.
32219929	10	8	dep	LED	1203:1205	arg1	blue					1198:1201	blue	1198:1201	blue	1198:1201	Nonesterified fatty acid levels in the yellow and blue LED groups decreased to the greatest extent compared to the start point.
32219929	1	9	theme	fluorescent	204:214	arg1	light					216:220	fluorescent light	204:220	fluorescent light	204:220	Light emitting diode (LED) is more energy efficient than incandescent or fluorescent light.
32219929	0	10	from	Effects	0:6	arg1	responses					104:112	physiological responses	90:112	physiological responses in dairy cattle	90:128	Effects of white, yellow, and blue colored LEDs on milk production, milk composition, and physiological responses in dairy cattle.
32219929	0	10	from	Effects	0:6	arg1	production					56:65	milk production	51:65	milk production	51:65	Effects of white, yellow, and blue colored LEDs on milk production, milk composition, and physiological responses in dairy cattle.
32219929	0	10	from	Effects	0:6	arg1	composition					73:83	milk composition	68:83	milk composition	68:83	Effects of white, yellow, and blue colored LEDs on milk production, milk composition, and physiological responses in dairy cattle.
32219929	7	11	theme	enzyme	929:934	arg1	activity					936:943	antioxidant enzyme activity	917:943	antioxidant enzyme activity	917:943	Extended exposure to blue LED light lowered antioxidant enzyme activity and insulin-like growth factor-1 levels.
32219929	6	12	theme	fat	713:715	arg1	contents					739:746	milk fat, protein, and lactose contents	708:746	milk fat, protein, and lactose contents	708:746	At the finish point, milk fat, protein, and lactose contents were the lowest in the blue LED group, whereas milk-urea-nitrogen levels were the highest in the yellow and blue LED groups.
32219929	6	12	theme	fat	713:715	arg1	lowest					757:762	lowest	757:762	lowest	757:762	At the finish point, milk fat, protein, and lactose contents were the lowest in the blue LED group, whereas milk-urea-nitrogen levels were the highest in the yellow and blue LED groups.
32219929	3	13	theme	natural	453:459	arg1	control					444:450	control	444:450	control (natural daylight)	444:469	According to milk production and parity, cows (n = 186) were allotted to four treatments: control (natural daylight), white, yellow, and blue LED groups.
32219929	3	13	theme	natural	453:459	arg1	daylight					461:468	natural daylight	453:468	natural daylight	453:468	According to milk production and parity, cows (n = 186) were allotted to four treatments: control (natural daylight), white, yellow, and blue LED groups.
32219929	6	14	theme	LED	861:863	arg1	groups					865:870	the yellow and blue LED groups	841:870	the yellow and blue LED groups	841:870	At the finish point, milk fat, protein, and lactose contents were the lowest in the blue LED group, whereas milk-urea-nitrogen levels were the highest in the yellow and blue LED groups.
32219929	9	15	theme	blue	1115:1118	arg1	group					1124:1128	the blue LED group	1111:1128	the blue LED group	1111:1128	Cortisol level was the highest in the blue LED group among the groups.
32219929	3	16	theme	white	472:476	arg1	treatments					432:441	four treatments	427:441	four treatments	427:441	According to milk production and parity, cows (n = 186) were allotted to four treatments: control (natural daylight), white, yellow, and blue LED groups.
32219929	6	17	theme	lactose	731:737	arg1	contents					739:746	milk fat, protein, and lactose contents	708:746	milk fat, protein, and lactose contents	708:746	At the finish point, milk fat, protein, and lactose contents were the lowest in the blue LED group, whereas milk-urea-nitrogen levels were the highest in the yellow and blue LED groups.
32219929	6	17	theme	lactose	731:737	arg1	lowest					757:762	lowest	757:762	lowest	757:762	At the finish point, milk fat, protein, and lactose contents were the lowest in the blue LED group, whereas milk-urea-nitrogen levels were the highest in the yellow and blue LED groups.
32219929	10	18	theme	greatest	1231:1238	arg1	extent					1240:1245	the greatest extent	1227:1245	the greatest extent	1227:1245	Nonesterified fatty acid levels in the yellow and blue LED groups decreased to the greatest extent compared to the start point.
32219929	5	19	theme	greater	608:614	arg1	rates					616:620	greater rates	608:620	greater rates of decline in milk production	608:650	Yellow and blue LED groups demonstrated greater rates of decline in milk production than control and white LED groups.
32219929	5	20	dep	control	657:663	arg1	groups					679:684	LED groups	675:684	LED groups	675:684	Yellow and blue LED groups demonstrated greater rates of decline in milk production than control and white LED groups.
32219929	7	21	dep	activity	936:943	arg1	levels					978:983	levels	978:983	levels	978:983	Extended exposure to blue LED light lowered antioxidant enzyme activity and insulin-like growth factor-1 levels.
32219929	1	22	theme	Light	131:135	arg1	diode					146:150	Light emitting diode	131:150	Light emitting diode (LED)	131:156	Light emitting diode (LED) is more energy efficient than incandescent or fluorescent light.
32219929	0	23	theme	milk	68:71	arg1	composition					73:83	milk composition	68:83	milk composition	68:83	Effects of white, yellow, and blue colored LEDs on milk production, milk composition, and physiological responses in dairy cattle.
32219929	7	24	theme	antioxidant	917:927	arg1	activity					936:943	antioxidant enzyme activity	917:943	antioxidant enzyme activity	917:943	Extended exposure to blue LED light lowered antioxidant enzyme activity and insulin-like growth factor-1 levels.
32219929	4	25	used	used	562:565	arg2	cows					521:524	40 cows	518:524	40 cows that had passed 57 day-in-milk	518:555	Of these, 40 cows that had passed 57 day-in-milk were used.
32219929	6	26	theme	yellow	845:850	arg1	groups					865:870	the yellow and blue LED groups	841:870	the yellow and blue LED groups	841:870	At the finish point, milk fat, protein, and lactose contents were the lowest in the blue LED group, whereas milk-urea-nitrogen levels were the highest in the yellow and blue LED groups.
32219929	1	27	theme	emitting	137:144	arg1	diode					146:150	Light emitting diode	131:150	Light emitting diode (LED)	131:156	Light emitting diode (LED) is more energy efficient than incandescent or fluorescent light.
32219929	6	28	theme	protein	718:724	arg1	contents					739:746	milk fat, protein, and lactose contents	708:746	milk fat, protein, and lactose contents	708:746	At the finish point, milk fat, protein, and lactose contents were the lowest in the blue LED group, whereas milk-urea-nitrogen levels were the highest in the yellow and blue LED groups.
32219929	6	28	theme	protein	718:724	arg1	lowest					757:762	lowest	757:762	lowest	757:762	At the finish point, milk fat, protein, and lactose contents were the lowest in the blue LED group, whereas milk-urea-nitrogen levels were the highest in the yellow and blue LED groups.
32219929	7	29	theme	LED	899:901	arg1	light					903:907	blue LED light	894:907	blue LED light	894:907	Extended exposure to blue LED light lowered antioxidant enzyme activity and insulin-like growth factor-1 levels.
32219929	11	30	theme	blue	1303:1306	arg1	light					1312:1316	blue LED light	1303:1316	blue LED light	1303:1316	These results suggest that blue LED light can decrease milk production and generate more stress than white and yellow LED lights.
32219929	2	31	theme	different	261:269	arg1	LEDs					279:282	different colored LEDs	261:282	different colored LEDs	261:282	This study was to evaluate effects of different colored LEDs on milk production, milk composition, and physiology of Holstein cow.
32219929	11	32	dep	LED	1394:1396	arg1	yellow					1387:1392	yellow	1387:1392	yellow	1387:1392	These results suggest that blue LED light can decrease milk production and generate more stress than white and yellow LED lights.
32219929	5	33	theme	LED	675:677	arg1	groups					679:684	LED groups	675:684	LED groups	675:684	Yellow and blue LED groups demonstrated greater rates of decline in milk production than control and white LED groups.
32219929	6	34	theme	milk-urea-nitrogen	795:812	arg1	levels					814:819	milk-urea-nitrogen levels	795:819	milk-urea-nitrogen levels	795:819	At the finish point, milk fat, protein, and lactose contents were the lowest in the blue LED group, whereas milk-urea-nitrogen levels were the highest in the yellow and blue LED groups.
32219929	6	34	theme	milk-urea-nitrogen	795:812	arg1	highest					830:836	highest	830:836	highest	830:836	At the finish point, milk fat, protein, and lactose contents were the lowest in the blue LED group, whereas milk-urea-nitrogen levels were the highest in the yellow and blue LED groups.
32219929	9	35	theme	Cortisol	1077:1084	arg1	level					1086:1090	Cortisol level	1077:1090	Cortisol level	1077:1090	Cortisol level was the highest in the blue LED group among the groups.
32219929	9	35	theme	Cortisol	1077:1084	arg1	highest					1100:1106	highest	1100:1106	highest	1100:1106	Cortisol level was the highest in the blue LED group among the groups.
32219929	3	36	theme	milk	367:370	arg1	production					372:381	milk production	367:381	milk production	367:381	According to milk production and parity, cows (n = 186) were allotted to four treatments: control (natural daylight), white, yellow, and blue LED groups.
32219929	5	37	theme	Yellow	568:573	arg1	groups					588:593	Yellow and blue LED groups	568:593	Yellow and blue LED groups	568:593	Yellow and blue LED groups demonstrated greater rates of decline in milk production than control and white LED groups.
32219929	10	38	theme	yellow	1187:1192	arg1	groups					1207:1212	the yellow and blue LED groups	1183:1212	the yellow and blue LED groups	1183:1212	Nonesterified fatty acid levels in the yellow and blue LED groups decreased to the greatest extent compared to the start point.
32219929	3	39	dep	treatments	432:441	arg1	control					444:450	control	444:450	control (natural daylight)	444:469	According to milk production and parity, cows (n = 186) were allotted to four treatments: control (natural daylight), white, yellow, and blue LED groups.
32219929	3	39	dep	treatments	432:441	arg1	daylight					461:468	natural daylight	453:468	natural daylight	453:468	According to milk production and parity, cows (n = 186) were allotted to four treatments: control (natural daylight), white, yellow, and blue LED groups.
32219929	10	40	from	levels	1173:1178	arg1	groups					1207:1212	the yellow and blue LED groups	1183:1212	the yellow and blue LED groups	1183:1212	Nonesterified fatty acid levels in the yellow and blue LED groups decreased to the greatest extent compared to the start point.
32219929	0	41	theme	white	11:15	arg1	LEDs					43:46	white, yellow, and blue colored LEDs	11:46	white, yellow, and blue colored LEDs	11:46	Effects of white, yellow, and blue colored LEDs on milk production, milk composition, and physiological responses in dairy cattle.
32219929	7	42	theme	blue	894:897	arg1	light					903:907	blue LED light	894:907	blue LED light	894:907	Extended exposure to blue LED light lowered antioxidant enzyme activity and insulin-like growth factor-1 levels.
32219929	8	43	theme	LED	1045:1047	arg1	groups					1049:1054	the white and blue LED groups	1026:1054	the white and blue LED groups	1026:1054	Prolactin concentrations were higher in the white and blue LED groups than in the control.
32219929	7	44	theme	Extended	873:880	arg1	exposure					882:889	Extended exposure	873:889	Extended exposure to blue LED light	873:907	Extended exposure to blue LED light lowered antioxidant enzyme activity and insulin-like growth factor-1 levels.
32219929	5	45	from	rates	616:620	arg1	production					641:650	milk production	636:650	milk production	636:650	Yellow and blue LED groups demonstrated greater rates of decline in milk production than control and white LED groups.
32219929	1	46	dep	diode	146:150	arg1	LED					153:155	LED	153:155	LED	153:155	Light emitting diode (LED) is more energy efficient than incandescent or fluorescent light.
32219929	2	47	theme	cow	349:351	arg1	physiology					326:335	physiology	326:335	physiology of Holstein cow	326:351	This study was to evaluate effects of different colored LEDs on milk production, milk composition, and physiology of Holstein cow.
32219929	2	47	theme	cow	349:351	arg1	production					292:301	milk production	287:301	milk production	287:301	This study was to evaluate effects of different colored LEDs on milk production, milk composition, and physiology of Holstein cow.
32219929	2	47	theme	cow	349:351	arg1	composition					309:319	milk composition	304:319	milk composition	304:319	This study was to evaluate effects of different colored LEDs on milk production, milk composition, and physiology of Holstein cow.
32219929	0	48	theme	yellow	18:23	arg1	LEDs					43:46	white, yellow, and blue colored LEDs	11:46	white, yellow, and blue colored LEDs	11:46	Effects of white, yellow, and blue colored LEDs on milk production, milk composition, and physiological responses in dairy cattle.
32219929	6	49	theme	LED	776:778	arg1	group					780:784	the blue LED group	767:784	the blue LED group	767:784	At the finish point, milk fat, protein, and lactose contents were the lowest in the blue LED group, whereas milk-urea-nitrogen levels were the highest in the yellow and blue LED groups.
32219929	0	50	theme	physiological	90:102	arg1	responses					104:112	physiological responses	90:112	physiological responses in dairy cattle	90:128	Effects of white, yellow, and blue colored LEDs on milk production, milk composition, and physiological responses in dairy cattle.
32219929	6	51	theme	milk	708:711	arg1	fat					713:715	milk fat	708:715	milk fat	708:715	At the finish point, milk fat, protein, and lactose contents were the lowest in the blue LED group, whereas milk-urea-nitrogen levels were the highest in the yellow and blue LED groups.
32219929	3	52	theme	blue	491:494	arg1	treatments					432:441	four treatments	427:441	four treatments	427:441	According to milk production and parity, cows (n = 186) were allotted to four treatments: control (natural daylight), white, yellow, and blue LED groups.
32219929	10	53	theme	fatty	1162:1166	arg1	levels					1173:1178	Nonesterified fatty acid levels	1148:1178	Nonesterified fatty acid levels in the yellow and blue LED groups	1148:1212	Nonesterified fatty acid levels in the yellow and blue LED groups decreased to the greatest extent compared to the start point.
32219929	2	54	theme	Holstein	340:347	arg1	cow					349:351	Holstein cow	340:351	Holstein cow	340:351	This study was to evaluate effects of different colored LEDs on milk production, milk composition, and physiology of Holstein cow.
32219929	0	55	theme	blue	30:33	arg1	LEDs					43:46	white, yellow, and blue colored LEDs	11:46	white, yellow, and blue colored LEDs	11:46	Effects of white, yellow, and blue colored LEDs on milk production, milk composition, and physiological responses in dairy cattle.
32219929	0	56	theme	dairy	117:121	arg1	cattle					123:128	dairy cattle	117:128	dairy cattle	117:128	Effects of white, yellow, and blue colored LEDs on milk production, milk composition, and physiological responses in dairy cattle.
32219929	5	57	theme	decline	625:631	arg1	rates					616:620	greater rates	608:620	greater rates of decline in milk production	608:650	Yellow and blue LED groups demonstrated greater rates of decline in milk production than control and white LED groups.
32219929	3	58	theme	yellow	479:484	arg1	treatments					432:441	four treatments	427:441	four treatments	427:441	According to milk production and parity, cows (n = 186) were allotted to four treatments: control (natural daylight), white, yellow, and blue LED groups.
32219929	10	59	theme	Nonesterified	1148:1160	arg1	levels					1173:1178	Nonesterified fatty acid levels	1148:1178	Nonesterified fatty acid levels in the yellow and blue LED groups	1148:1212	Nonesterified fatty acid levels in the yellow and blue LED groups decreased to the greatest extent compared to the start point.
32219929	2	60	theme	milk	287:290	arg1	production					292:301	milk production	287:301	milk production	287:301	This study was to evaluate effects of different colored LEDs on milk production, milk composition, and physiology of Holstein cow.
32219929	5	61	theme	LED	584:586	arg1	groups					588:593	Yellow and blue LED groups	568:593	Yellow and blue LED groups	568:593	Yellow and blue LED groups demonstrated greater rates of decline in milk production than control and white LED groups.
32219929	0	62	from	composition	73:83	arg1	cattle					123:128	dairy cattle	117:128	dairy cattle	117:128	Effects of white, yellow, and blue colored LEDs on milk production, milk composition, and physiological responses in dairy cattle.
32219929	11	63	theme	white	1377:1381	arg1	lights					1398:1403	white and yellow LED lights	1377:1403	white and yellow LED lights	1377:1403	These results suggest that blue LED light can decrease milk production and generate more stress than white and yellow LED lights.
32219929	6	64	dep	LED	776:778	arg1	blue					771:774	blue	771:774	blue	771:774	At the finish point, milk fat, protein, and lactose contents were the lowest in the blue LED group, whereas milk-urea-nitrogen levels were the highest in the yellow and blue LED groups.
32219929	10	65	theme	start	1263:1267	arg1	point					1269:1273	the start point	1259:1273	the start point	1259:1273	Nonesterified fatty acid levels in the yellow and blue LED groups decreased to the greatest extent compared to the start point.
32219929	6	66	theme	finish	694:699	arg1	point					701:705	the finish point	690:705	the finish point	690:705	At the finish point, milk fat, protein, and lactose contents were the lowest in the blue LED group, whereas milk-urea-nitrogen levels were the highest in the yellow and blue LED groups.
32219929	2	67	theme	LEDs	279:282	arg1	effects					250:256	effects	250:256	effects of different colored LEDs on milk production, milk composition, and physiology of Holstein cow	250:351	This study was to evaluate effects of different colored LEDs on milk production, milk composition, and physiology of Holstein cow.
32219929	11	68	theme	more	1360:1363	arg1	stress					1365:1370	more stress	1360:1370	more stress than white and yellow LED lights	1360:1403	These results suggest that blue LED light can decrease milk production and generate more stress than white and yellow LED lights.
32219929	0	69	from	responses	104:112	arg1	cattle					123:128	dairy cattle	117:128	dairy cattle	117:128	Effects of white, yellow, and blue colored LEDs on milk production, milk composition, and physiological responses in dairy cattle.
32219929	6	70	dep	LED	861:863	arg1	blue					856:859	blue	856:859	blue	856:859	At the finish point, milk fat, protein, and lactose contents were the lowest in the blue LED group, whereas milk-urea-nitrogen levels were the highest in the yellow and blue LED groups.
32219929	11	71	theme	milk	1331:1334	arg1	production					1336:1345	milk production	1331:1345	milk production	1331:1345	These results suggest that blue LED light can decrease milk production and generate more stress than white and yellow LED lights.
32219929	2	72	dep	different	261:269	arg1	colored					271:277	colored	271:277	colored	271:277	This study was to evaluate effects of different colored LEDs on milk production, milk composition, and physiology of Holstein cow.
32219929	0	73	theme	LEDs	43:46	arg1	Effects					0:6	Effects	0:6	Effects of white, yellow, and blue colored LEDs on milk production, milk composition, and physiological responses in dairy cattle	0:128	Effects of white, yellow, and blue colored LEDs on milk production, milk composition, and physiological responses in dairy cattle.
32219929	0	74	from	production	56:65	arg1	cattle					123:128	dairy cattle	117:128	dairy cattle	117:128	Effects of white, yellow, and blue colored LEDs on milk production, milk composition, and physiological responses in dairy cattle.
32219929	11	75	theme	LED	1308:1310	arg1	light					1312:1316	blue LED light	1303:1316	blue LED light	1303:1316	These results suggest that blue LED light can decrease milk production and generate more stress than white and yellow LED lights.
32219929	0	76	theme	colored	35:41	arg1	LEDs					43:46	white, yellow, and blue colored LEDs	11:46	white, yellow, and blue colored LEDs	11:46	Effects of white, yellow, and blue colored LEDs on milk production, milk composition, and physiological responses in dairy cattle.
32219929	11	77	theme	LED	1394:1396	arg1	lights					1398:1403	white and yellow LED lights	1377:1403	white and yellow LED lights	1377:1403	These results suggest that blue LED light can decrease milk production and generate more stress than white and yellow LED lights.
32219929	8	78	theme	white	1030:1034	arg1	groups					1049:1054	the white and blue LED groups	1026:1054	the white and blue LED groups	1026:1054	Prolactin concentrations were higher in the white and blue LED groups than in the control.
32219929	8	79	dep	LED	1045:1047	arg1	blue					1040:1043	blue	1040:1043	blue	1040:1043	Prolactin concentrations were higher in the white and blue LED groups than in the control.
32219929	2	80	theme	milk	304:307	arg1	composition					309:319	milk composition	304:319	milk composition	304:319	This study was to evaluate effects of different colored LEDs on milk production, milk composition, and physiology of Holstein cow.
32219929	2	81	from	effects	250:256	arg1	physiology					326:335	physiology	326:335	physiology of Holstein cow	326:351	This study was to evaluate effects of different colored LEDs on milk production, milk composition, and physiology of Holstein cow.
32219929	2	81	from	effects	250:256	arg1	production					292:301	milk production	287:301	milk production	287:301	This study was to evaluate effects of different colored LEDs on milk production, milk composition, and physiology of Holstein cow.
32219929	2	81	from	effects	250:256	arg1	composition					309:319	milk composition	304:319	milk composition	304:319	This study was to evaluate effects of different colored LEDs on milk production, milk composition, and physiology of Holstein cow.
32219929	5	82	dep	LED	584:586	arg1	blue					579:582	blue	579:582	blue	579:582	Yellow and blue LED groups demonstrated greater rates of decline in milk production than control and white LED groups.
33515570	4	0	with	interaction	933:943	arg1	network					954:960	the network	950:960	the network	950:960	Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Analysis (EDAX) and Transmission Electron Microscopy (TEM) results confirmed uniform mineralization of BG particles, and their synergetic interaction with the network.
33515570	3	1	theme	BG	680:681	arg1	nanoparticles					683:695	the BG nanoparticles	676:695	the BG nanoparticles acting as pseudo crosslink junctions	676:732	Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA) confirmed this associative interaction which resulted in the formation of a hybrid crosslinked network with the BG nanoparticles acting as pseudo crosslink junctions.
33515570	10	2	theme	hydrophilic	1728:1738	arg1	surfaces					1740:1747	hydrophilic surfaces	1728:1747	hydrophilic surfaces	1728:1747	FDA/PI on the hydrogel portrayed preferential U2OS cell adhesion on hydrophobic hydroxyapatite layer compared to hydrophilic surfaces, thereby promising the regeneration of both soft and hard tissues.
33515570	4	3	theme	uniform	872:878	arg1	mineralization					880:893	uniform mineralization	872:893	uniform mineralization of BG particles	872:909	Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Analysis (EDAX) and Transmission Electron Microscopy (TEM) results confirmed uniform mineralization of BG particles, and their synergetic interaction with the network.
33515570	3	4	theme	thermogravimetric	535:551	arg1	TGA					563:565	TGA	563:565	TGA	563:565	Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA) confirmed this associative interaction which resulted in the formation of a hybrid crosslinked network with the BG nanoparticles acting as pseudo crosslink junctions.
33515570	3	4	theme	thermogravimetric	535:551	arg1	analysis					553:560	thermogravimetric analysis	535:560	thermogravimetric analysis (TGA)	535:566	Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA) confirmed this associative interaction which resulted in the formation of a hybrid crosslinked network with the BG nanoparticles acting as pseudo crosslink junctions.
33515570	3	5	theme	pseudo	707:712	arg1	junctions					724:732	pseudo crosslink junctions	707:732	pseudo crosslink junctions	707:732	Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA) confirmed this associative interaction which resulted in the formation of a hybrid crosslinked network with the BG nanoparticles acting as pseudo crosslink junctions.
33515570	5	6	from	tests	988:992	arg1	CPBG					997:1000	CPBG	997:1000	CPBG	997:1000	The in-vitro bioactivity tests on CPBG indicated the formation of bone-like hydroxyapatite (Ca10(PO4)6(OH)2) microcrystals on its surface after interaction with simulated body fluid.
33515570	1	7	theme	hydrogel	156:163	arg1	design					100:105	the design	96:105	the design of a novel collagen/pectin (CP) hybrid composite hydrogel (CPBG) containing in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment	96:279	In this report, we discuss the design of a novel collagen/pectin (CP) hybrid composite hydrogel (CPBG) containing in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment.
33515570	7	8	theme	gel	1369:1371	arg1	behavior					1382:1389	gel swelling behavior	1369:1389	gel swelling behavior	1369:1389	The AmB release kinetics from the hydrogel followed the Fickian mechanism and showed direct proportionality to gel swelling behavior.
33515570	2	9	theme	Systematic	282:291	arg1	analysis					293:300	Systematic analysis	282:300	Systematic analysis of the CP sol	282:314	Systematic analysis of the CP sol revealed collagen and pectin molecules interacted regardless of both possessing similar net negative charge through the mechanism of surface patch binding interaction.
33515570	4	10	theme	synergetic	922:931	arg1	interaction					933:943	their synergetic interaction	916:943	their synergetic interaction with the network	916:960	Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Analysis (EDAX) and Transmission Electron Microscopy (TEM) results confirmed uniform mineralization of BG particles, and their synergetic interaction with the network.
33515570	6	11	theme	drug	1195:1198	arg1	AmB					1216:1218	AmB	1216:1218	AmB	1216:1218	This hydrogel was loaded with a model antifungal drug amphotericin-B (AmB) and tested against Candida albicans.
33515570	6	11	theme	drug	1195:1198	arg1	amphotericin-B					1200:1213	a model antifungal drug amphotericin-B	1176:1213	a model antifungal drug amphotericin-B (AmB)	1176:1219	This hydrogel was loaded with a model antifungal drug amphotericin-B (AmB) and tested against Candida albicans.
33515570	1	12	theme	mineralized	191:201	arg1	particles					224:232	in-situ mineralized bioactive glass (BG) particles	183:232	in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment	183:279	In this report, we discuss the design of a novel collagen/pectin (CP) hybrid composite hydrogel (CPBG) containing in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment.
33515570	8	13	theme	viscoelastic	1426:1437	arg1	compatibility					1439:1451	the viscoelastic compatibility	1422:1451	the viscoelastic compatibility of CPBG for the mechanical load bearing applications	1422:1504	Rheological analysis revealed the viscoelastic compatibility of CPBG for the mechanical load bearing applications.
33515570	6	14	theme	model	1178:1182	arg1	AmB					1216:1218	AmB	1216:1218	AmB	1216:1218	This hydrogel was loaded with a model antifungal drug amphotericin-B (AmB) and tested against Candida albicans.
33515570	6	14	theme	model	1178:1182	arg1	amphotericin-B					1200:1213	a model antifungal drug amphotericin-B	1176:1213	a model antifungal drug amphotericin-B (AmB)	1176:1219	This hydrogel was loaded with a model antifungal drug amphotericin-B (AmB) and tested against Candida albicans.
33515570	5	15	theme	hydroxyapatite	1039:1052	arg1	microcrystals					1072:1084	bone-like hydroxyapatite (Ca10(PO4)6(OH)2) microcrystals	1029:1084	bone-like hydroxyapatite (Ca10(PO4)6(OH)2) microcrystals	1029:1084	The in-vitro bioactivity tests on CPBG indicated the formation of bone-like hydroxyapatite (Ca10(PO4)6(OH)2) microcrystals on its surface after interaction with simulated body fluid.
33515570	10	16	theme	tissues	1807:1813	arg1	regeneration					1772:1783	the regeneration	1768:1783	the regeneration of both soft and hard tissues	1768:1813	FDA/PI on the hydrogel portrayed preferential U2OS cell adhesion on hydrophobic hydroxyapatite layer compared to hydrophilic surfaces, thereby promising the regeneration of both soft and hard tissues.
33515570	4	17	dep	Microscopy	753:762	arg1	results					854:860	results	854:860	results	854:860	Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Analysis (EDAX) and Transmission Electron Microscopy (TEM) results confirmed uniform mineralization of BG particles, and their synergetic interaction with the network.
33515570	4	18	theme	particles	901:909	arg1	mineralization					880:893	uniform mineralization	872:893	uniform mineralization of BG particles	872:909	Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Analysis (EDAX) and Transmission Electron Microscopy (TEM) results confirmed uniform mineralization of BG particles, and their synergetic interaction with the network.
33515570	4	18	theme	particles	901:909	arg1	interaction					933:943	their synergetic interaction	916:943	their synergetic interaction with the network	916:960	Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Analysis (EDAX) and Transmission Electron Microscopy (TEM) results confirmed uniform mineralization of BG particles, and their synergetic interaction with the network.
33515570	1	19	theme	collagen/pectin	118:132	arg1	CPBG					166:169	CPBG	166:169	CPBG	166:169	In this report, we discuss the design of a novel collagen/pectin (CP) hybrid composite hydrogel (CPBG) containing in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment.
33515570	1	19	theme	collagen/pectin	118:132	arg1	hydrogel					156:163	a novel collagen/pectin (CP) hybrid composite hydrogel	110:163	a novel collagen/pectin (CP) hybrid composite hydrogel (CPBG) containing in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment	110:279	In this report, we discuss the design of a novel collagen/pectin (CP) hybrid composite hydrogel (CPBG) containing in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment.
33515570	10	20	theme	hydrophobic	1683:1693	arg1	layer					1710:1714	hydrophobic hydroxyapatite layer	1683:1714	hydrophobic hydroxyapatite layer	1683:1714	FDA/PI on the hydrogel portrayed preferential U2OS cell adhesion on hydrophobic hydroxyapatite layer compared to hydrophilic surfaces, thereby promising the regeneration of both soft and hard tissues.
33515570	5	21	theme	microcrystals	1072:1084	arg1	formation					1016:1024	the formation	1012:1024	the formation of bone-like hydroxyapatite (Ca10(PO4)6(OH)2) microcrystals on its surface	1012:1099	The in-vitro bioactivity tests on CPBG indicated the formation of bone-like hydroxyapatite (Ca10(PO4)6(OH)2) microcrystals on its surface after interaction with simulated body fluid.
33515570	1	22	theme	CP	135:136	arg1	CPBG					166:169	CPBG	166:169	CPBG	166:169	In this report, we discuss the design of a novel collagen/pectin (CP) hybrid composite hydrogel (CPBG) containing in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment.
33515570	1	22	theme	CP	135:136	arg1	hydrogel					156:163	a novel collagen/pectin (CP) hybrid composite hydrogel	110:163	a novel collagen/pectin (CP) hybrid composite hydrogel (CPBG) containing in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment	110:279	In this report, we discuss the design of a novel collagen/pectin (CP) hybrid composite hydrogel (CPBG) containing in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment.
33515570	2	23	theme	interaction	471:481	arg1	mechanism					436:444	the mechanism	432:444	the mechanism of surface patch binding interaction	432:481	Systematic analysis of the CP sol revealed collagen and pectin molecules interacted regardless of both possessing similar net negative charge through the mechanism of surface patch binding interaction.
33515570	8	24	theme	mechanical	1469:1478	arg1	load					1480:1483	the mechanical load	1465:1483	the mechanical load bearing applications	1465:1504	Rheological analysis revealed the viscoelastic compatibility of CPBG for the mechanical load bearing applications.
33515570	1	25	theme	hybrid	139:144	arg1	CPBG					166:169	CPBG	166:169	CPBG	166:169	In this report, we discuss the design of a novel collagen/pectin (CP) hybrid composite hydrogel (CPBG) containing in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment.
33515570	1	25	theme	hybrid	139:144	arg1	hydrogel					156:163	a novel collagen/pectin (CP) hybrid composite hydrogel	110:163	a novel collagen/pectin (CP) hybrid composite hydrogel (CPBG) containing in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment	110:279	In this report, we discuss the design of a novel collagen/pectin (CP) hybrid composite hydrogel (CPBG) containing in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment.
33515570	5	26	theme	OH	1066:1067	arg1	Ca10					1055:1058	Ca10(PO4)6(OH)2	1055:1069	Ca10(PO4)6(OH)2	1055:1069	The in-vitro bioactivity tests on CPBG indicated the formation of bone-like hydroxyapatite (Ca10(PO4)6(OH)2) microcrystals on its surface after interaction with simulated body fluid.
33515570	5	26	theme	OH	1066:1067	arg1	hydroxyapatite					1039:1052	hydroxyapatite	1039:1052	bone-like hydroxyapatite (Ca10(PO4)6(OH)2) microcrystals	1029:1084	The in-vitro bioactivity tests on CPBG indicated the formation of bone-like hydroxyapatite (Ca10(PO4)6(OH)2) microcrystals on its surface after interaction with simulated body fluid.
33515570	9	27	theme	appreciable	1538:1548	arg1	compatibility					1550:1562	appreciable compatibility	1538:1562	appreciable compatibility of the hydrogel against U2OS and HaCaT cell lines	1538:1612	Cell viability tests indicated appreciable compatibility of the hydrogel against U2OS and HaCaT cell lines.
33515570	7	28	from	hydrogel	1292:1299	arg1	kinetics					1274:1281	The AmB release kinetics	1258:1281	The AmB release kinetics from the hydrogel	1258:1299	The AmB release kinetics from the hydrogel followed the Fickian mechanism and showed direct proportionality to gel swelling behavior.
33515570	4	29	theme	Energy	771:776	arg1	Analysis					795:802	Energy Dispersive X-Ray Analysis	771:802	Energy Dispersive X-Ray Analysis (EDAX)	771:809	Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Analysis (EDAX) and Transmission Electron Microscopy (TEM) results confirmed uniform mineralization of BG particles, and their synergetic interaction with the network.
33515570	4	29	theme	Energy	771:776	arg1	EDAX					805:808	EDAX	805:808	EDAX	805:808	Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Analysis (EDAX) and Transmission Electron Microscopy (TEM) results confirmed uniform mineralization of BG particles, and their synergetic interaction with the network.
33515570	10	30	theme	U2OS	1661:1664	arg1	adhesion					1671:1678	preferential U2OS cell adhesion	1648:1678	preferential U2OS cell adhesion on hydrophobic hydroxyapatite layer compared to hydrophilic surfaces, thereby promising the regeneration of both soft and hard tissues	1648:1813	FDA/PI on the hydrogel portrayed preferential U2OS cell adhesion on hydrophobic hydroxyapatite layer compared to hydrophilic surfaces, thereby promising the regeneration of both soft and hard tissues.
33515570	5	31	theme	bioactivity	976:986	arg1	tests					988:992	The in-vitro bioactivity tests	963:992	The in-vitro bioactivity tests on CPBG	963:1000	The in-vitro bioactivity tests on CPBG indicated the formation of bone-like hydroxyapatite (Ca10(PO4)6(OH)2) microcrystals on its surface after interaction with simulated body fluid.
33515570	4	32	theme	Electron	744:751	arg1	Microscopy					753:762	Scanning Electron Microscopy	735:762	Scanning Electron Microscopy (SEM)	735:768	Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Analysis (EDAX) and Transmission Electron Microscopy (TEM) results confirmed uniform mineralization of BG particles, and their synergetic interaction with the network.
33515570	4	32	theme	Electron	744:751	arg1	SEM					765:767	SEM	765:767	SEM	765:767	Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Analysis (EDAX) and Transmission Electron Microscopy (TEM) results confirmed uniform mineralization of BG particles, and their synergetic interaction with the network.
33515570	5	33	theme	body	1134:1137	arg1	fluid					1139:1143	simulated body fluid	1124:1143	simulated body fluid	1124:1143	The in-vitro bioactivity tests on CPBG indicated the formation of bone-like hydroxyapatite (Ca10(PO4)6(OH)2) microcrystals on its surface after interaction with simulated body fluid.
33515570	9	34	theme	hydrogel	1571:1578	arg1	compatibility					1550:1562	appreciable compatibility	1538:1562	appreciable compatibility of the hydrogel against U2OS and HaCaT cell lines	1538:1612	Cell viability tests indicated appreciable compatibility of the hydrogel against U2OS and HaCaT cell lines.
33515570	2	35	theme	net	404:406	arg1	charge					417:422	similar net negative charge	396:422	similar net negative charge	396:422	Systematic analysis of the CP sol revealed collagen and pectin molecules interacted regardless of both possessing similar net negative charge through the mechanism of surface patch binding interaction.
33515570	0	36	theme	surface	23:29	arg1	hydrogel					59:66	surface patch bound collagen-pectin hydrogel	23:66	surface patch bound collagen-pectin hydrogel	23:66	Bioactivity reinforced surface patch bound collagen-pectin hydrogel.
33515570	1	37	theme	3D	261:262	arg1	environment					269:279	an integrative 3D cell environment	246:279	an integrative 3D cell environment	246:279	In this report, we discuss the design of a novel collagen/pectin (CP) hybrid composite hydrogel (CPBG) containing in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment.
33515570	7	38	theme	AmB	1262:1264	arg1	kinetics					1274:1281	The AmB release kinetics	1258:1281	The AmB release kinetics from the hydrogel	1258:1299	The AmB release kinetics from the hydrogel followed the Fickian mechanism and showed direct proportionality to gel swelling behavior.
33515570	2	39	theme	patch	457:461	arg1	interaction					471:481	surface patch binding interaction	449:481	surface patch binding interaction	449:481	Systematic analysis of the CP sol revealed collagen and pectin molecules interacted regardless of both possessing similar net negative charge through the mechanism of surface patch binding interaction.
33515570	0	40	theme	bound	37:41	arg1	hydrogel					59:66	surface patch bound collagen-pectin hydrogel	23:66	surface patch bound collagen-pectin hydrogel	23:66	Bioactivity reinforced surface patch bound collagen-pectin hydrogel.
33515570	9	41	theme	HaCaT	1597:1601	arg1	lines					1608:1612	HaCaT cell lines	1597:1612	HaCaT cell lines	1597:1612	Cell viability tests indicated appreciable compatibility of the hydrogel against U2OS and HaCaT cell lines.
33515570	4	42	theme	Transmission	815:826	arg1	TEM					849:851	TEM	849:851	TEM	849:851	Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Analysis (EDAX) and Transmission Electron Microscopy (TEM) results confirmed uniform mineralization of BG particles, and their synergetic interaction with the network.
33515570	4	42	theme	Transmission	815:826	arg1	Microscopy					837:846	Transmission Electron Microscopy	815:846	Transmission Electron Microscopy (TEM)	815:852	Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Analysis (EDAX) and Transmission Electron Microscopy (TEM) results confirmed uniform mineralization of BG particles, and their synergetic interaction with the network.
33515570	2	43	theme	sol	312:314	arg1	analysis					293:300	Systematic analysis	282:300	Systematic analysis of the CP sol	282:314	Systematic analysis of the CP sol revealed collagen and pectin molecules interacted regardless of both possessing similar net negative charge through the mechanism of surface patch binding interaction.
33515570	5	44	theme	bone-like	1029:1037	arg1	microcrystals					1072:1084	bone-like hydroxyapatite (Ca10(PO4)6(OH)2) microcrystals	1029:1084	bone-like hydroxyapatite (Ca10(PO4)6(OH)2) microcrystals	1029:1084	The in-vitro bioactivity tests on CPBG indicated the formation of bone-like hydroxyapatite (Ca10(PO4)6(OH)2) microcrystals on its surface after interaction with simulated body fluid.
33515570	3	45	link	crosslinked	651:661	arg1	network					663:669	a hybrid crosslinked network	642:669	a hybrid crosslinked network with the BG nanoparticles acting as pseudo crosslink junctions	642:732	Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA) confirmed this associative interaction which resulted in the formation of a hybrid crosslinked network with the BG nanoparticles acting as pseudo crosslink junctions.
33515570	4	46	theme	X-Ray	789:793	arg1	Analysis					795:802	Energy Dispersive X-Ray Analysis	771:802	Energy Dispersive X-Ray Analysis (EDAX)	771:809	Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Analysis (EDAX) and Transmission Electron Microscopy (TEM) results confirmed uniform mineralization of BG particles, and their synergetic interaction with the network.
33515570	4	46	theme	X-Ray	789:793	arg1	EDAX					805:808	EDAX	805:808	EDAX	805:808	Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Analysis (EDAX) and Transmission Electron Microscopy (TEM) results confirmed uniform mineralization of BG particles, and their synergetic interaction with the network.
33515570	1	47	theme	bioactive	203:211	arg1	particles					224:232	in-situ mineralized bioactive glass (BG) particles	183:232	in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment	183:279	In this report, we discuss the design of a novel collagen/pectin (CP) hybrid composite hydrogel (CPBG) containing in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment.
33515570	10	48	from	adhesion	1671:1678	arg1	layer					1710:1714	hydrophobic hydroxyapatite layer	1683:1714	hydrophobic hydroxyapatite layer	1683:1714	FDA/PI on the hydrogel portrayed preferential U2OS cell adhesion on hydrophobic hydroxyapatite layer compared to hydrophilic surfaces, thereby promising the regeneration of both soft and hard tissues.
33515570	9	49	theme	Cell	1507:1510	arg1	tests					1522:1526	Cell viability tests	1507:1526	Cell viability tests	1507:1526	Cell viability tests indicated appreciable compatibility of the hydrogel against U2OS and HaCaT cell lines.
33515570	3	50	theme	network	663:669	arg1	formation					629:637	the formation	625:637	the formation of a hybrid crosslinked network with the BG nanoparticles acting as pseudo crosslink junctions	625:732	Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA) confirmed this associative interaction which resulted in the formation of a hybrid crosslinked network with the BG nanoparticles acting as pseudo crosslink junctions.
33515570	2	51	theme	pectin	338:343	arg1	molecules					345:353	collagen and pectin molecules	325:353	molecules	345:353	Systematic analysis of the CP sol revealed collagen and pectin molecules interacted regardless of both possessing similar net negative charge through the mechanism of surface patch binding interaction.
33515570	6	52	theme	Candida	1240:1246	arg1	albicans					1248:1255	Candida albicans	1240:1255	Candida albicans	1240:1255	This hydrogel was loaded with a model antifungal drug amphotericin-B (AmB) and tested against Candida albicans.
33515570	2	53	theme	collagen	325:332	arg1	molecules					345:353	collagen and pectin molecules	325:353	molecules	345:353	Systematic analysis of the CP sol revealed collagen and pectin molecules interacted regardless of both possessing similar net negative charge through the mechanism of surface patch binding interaction.
33515570	3	54	dep	crosslinked	651:661	arg1	hybrid					644:649	hybrid	644:649	hybrid	644:649	Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA) confirmed this associative interaction which resulted in the formation of a hybrid crosslinked network with the BG nanoparticles acting as pseudo crosslink junctions.
33515570	10	55	from	FDA/PI	1615:1620	arg1	hydrogel					1629:1636	the hydrogel	1625:1636	the hydrogel	1625:1636	FDA/PI on the hydrogel portrayed preferential U2OS cell adhesion on hydrophobic hydroxyapatite layer compared to hydrophilic surfaces, thereby promising the regeneration of both soft and hard tissues.
33515570	5	56	with	interaction	1107:1117	arg1	fluid					1139:1143	simulated body fluid	1124:1143	simulated body fluid	1124:1143	The in-vitro bioactivity tests on CPBG indicated the formation of bone-like hydroxyapatite (Ca10(PO4)6(OH)2) microcrystals on its surface after interaction with simulated body fluid.
33515570	3	57	with	network	663:669	arg1	nanoparticles					683:695	the BG nanoparticles	676:695	the BG nanoparticles acting as pseudo crosslink junctions	676:732	Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA) confirmed this associative interaction which resulted in the formation of a hybrid crosslinked network with the BG nanoparticles acting as pseudo crosslink junctions.
33515570	1	58	theme	composite	146:154	arg1	CPBG					166:169	CPBG	166:169	CPBG	166:169	In this report, we discuss the design of a novel collagen/pectin (CP) hybrid composite hydrogel (CPBG) containing in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment.
33515570	1	58	theme	composite	146:154	arg1	hydrogel					156:163	a novel collagen/pectin (CP) hybrid composite hydrogel	110:163	a novel collagen/pectin (CP) hybrid composite hydrogel (CPBG) containing in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment	110:279	In this report, we discuss the design of a novel collagen/pectin (CP) hybrid composite hydrogel (CPBG) containing in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment.
33515570	4	59	theme	Electron	828:835	arg1	TEM					849:851	TEM	849:851	TEM	849:851	Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Analysis (EDAX) and Transmission Electron Microscopy (TEM) results confirmed uniform mineralization of BG particles, and their synergetic interaction with the network.
33515570	4	59	theme	Electron	828:835	arg1	Microscopy					837:846	Transmission Electron Microscopy	815:846	Transmission Electron Microscopy (TEM)	815:852	Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Analysis (EDAX) and Transmission Electron Microscopy (TEM) results confirmed uniform mineralization of BG particles, and their synergetic interaction with the network.
33515570	3	60	theme	crosslink	714:722	arg1	junctions					724:732	pseudo crosslink junctions	707:732	pseudo crosslink junctions	707:732	Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA) confirmed this associative interaction which resulted in the formation of a hybrid crosslinked network with the BG nanoparticles acting as pseudo crosslink junctions.
33515570	10	61	theme	hard	1802:1805	arg1	tissues					1807:1813	both soft and hard tissues	1788:1813	both soft and hard tissues	1788:1813	FDA/PI on the hydrogel portrayed preferential U2OS cell adhesion on hydrophobic hydroxyapatite layer compared to hydrophilic surfaces, thereby promising the regeneration of both soft and hard tissues.
33515570	7	62	theme	swelling	1373:1380	arg1	behavior					1382:1389	gel swelling behavior	1369:1389	gel swelling behavior	1369:1389	The AmB release kinetics from the hydrogel followed the Fickian mechanism and showed direct proportionality to gel swelling behavior.
33515570	10	63	theme	soft	1793:1796	arg1	tissues					1807:1813	both soft and hard tissues	1788:1813	both soft and hard tissues	1788:1813	FDA/PI on the hydrogel portrayed preferential U2OS cell adhesion on hydrophobic hydroxyapatite layer compared to hydrophilic surfaces, thereby promising the regeneration of both soft and hard tissues.
33515570	3	64	theme	associative	583:593	arg1	interaction					595:605	this associative interaction	578:605	this associative interaction which resulted in the formation of a hybrid crosslinked network with the BG nanoparticles acting as pseudo crosslink junctions	578:732	Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA) confirmed this associative interaction which resulted in the formation of a hybrid crosslinked network with the BG nanoparticles acting as pseudo crosslink junctions.
33515570	5	65	from	formation	1016:1024	arg1	surface					1093:1099	its surface	1089:1099	its surface	1089:1099	The in-vitro bioactivity tests on CPBG indicated the formation of bone-like hydroxyapatite (Ca10(PO4)6(OH)2) microcrystals on its surface after interaction with simulated body fluid.
33515570	1	66	theme	in-situ	183:189	arg1	particles					224:232	in-situ mineralized bioactive glass (BG) particles	183:232	in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment	183:279	In this report, we discuss the design of a novel collagen/pectin (CP) hybrid composite hydrogel (CPBG) containing in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment.
33515570	6	67	theme	antifungal	1184:1193	arg1	AmB					1216:1218	AmB	1216:1218	AmB	1216:1218	This hydrogel was loaded with a model antifungal drug amphotericin-B (AmB) and tested against Candida albicans.
33515570	6	67	theme	antifungal	1184:1193	arg1	amphotericin-B					1200:1213	a model antifungal drug amphotericin-B	1176:1213	a model antifungal drug amphotericin-B (AmB)	1176:1219	This hydrogel was loaded with a model antifungal drug amphotericin-B (AmB) and tested against Candida albicans.
33515570	8	68	theme	CPBG	1456:1459	arg1	compatibility					1439:1451	the viscoelastic compatibility	1422:1451	the viscoelastic compatibility of CPBG for the mechanical load bearing applications	1422:1504	Rheological analysis revealed the viscoelastic compatibility of CPBG for the mechanical load bearing applications.
33515570	2	69	contain	possessing	385:394	arg2	charge					417:422	similar net negative charge	396:422	similar net negative charge	396:422	Systematic analysis of the CP sol revealed collagen and pectin molecules interacted regardless of both possessing similar net negative charge through the mechanism of surface patch binding interaction.
33515570	2	69	contain	possessing	385:394	arg1	both					380:383	both	380:383	both	380:383	Systematic analysis of the CP sol revealed collagen and pectin molecules interacted regardless of both possessing similar net negative charge through the mechanism of surface patch binding interaction.
33515570	1	70	theme	novel	112:116	arg1	CPBG					166:169	CPBG	166:169	CPBG	166:169	In this report, we discuss the design of a novel collagen/pectin (CP) hybrid composite hydrogel (CPBG) containing in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment.
33515570	1	70	theme	novel	112:116	arg1	hydrogel					156:163	a novel collagen/pectin (CP) hybrid composite hydrogel	110:163	a novel collagen/pectin (CP) hybrid composite hydrogel (CPBG) containing in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment	110:279	In this report, we discuss the design of a novel collagen/pectin (CP) hybrid composite hydrogel (CPBG) containing in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment.
33515570	4	71	theme	BG	898:899	arg1	particles					901:909	BG particles	898:909	BG particles	898:909	Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Analysis (EDAX) and Transmission Electron Microscopy (TEM) results confirmed uniform mineralization of BG particles, and their synergetic interaction with the network.
33515570	7	72	theme	Fickian	1314:1320	arg1	mechanism					1322:1330	the Fickian mechanism	1310:1330	the Fickian mechanism	1310:1330	The AmB release kinetics from the hydrogel followed the Fickian mechanism and showed direct proportionality to gel swelling behavior.
33515570	9	73	theme	viability	1512:1520	arg1	tests					1522:1526	Cell viability tests	1507:1526	Cell viability tests	1507:1526	Cell viability tests indicated appreciable compatibility of the hydrogel against U2OS and HaCaT cell lines.
33515570	2	74	theme	binding	463:469	arg1	interaction					471:481	surface patch binding interaction	449:481	surface patch binding interaction	449:481	Systematic analysis of the CP sol revealed collagen and pectin molecules interacted regardless of both possessing similar net negative charge through the mechanism of surface patch binding interaction.
33515570	1	75	contain	containing	172:181	arg2	particles					224:232	in-situ mineralized bioactive glass (BG) particles	183:232	in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment	183:279	In this report, we discuss the design of a novel collagen/pectin (CP) hybrid composite hydrogel (CPBG) containing in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment.
33515570	1	75	contain	containing	172:181	arg1	CPBG					166:169	CPBG	166:169	CPBG	166:169	In this report, we discuss the design of a novel collagen/pectin (CP) hybrid composite hydrogel (CPBG) containing in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment.
33515570	1	75	contain	containing	172:181	arg1	hydrogel					156:163	a novel collagen/pectin (CP) hybrid composite hydrogel	110:163	a novel collagen/pectin (CP) hybrid composite hydrogel (CPBG) containing in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment	110:279	In this report, we discuss the design of a novel collagen/pectin (CP) hybrid composite hydrogel (CPBG) containing in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment.
33515570	5	76	theme	in-vitro	967:974	arg1	tests					988:992	The in-vitro bioactivity tests	963:992	The in-vitro bioactivity tests on CPBG	963:1000	The in-vitro bioactivity tests on CPBG indicated the formation of bone-like hydroxyapatite (Ca10(PO4)6(OH)2) microcrystals on its surface after interaction with simulated body fluid.
33515570	10	77	theme	preferential	1648:1659	arg1	adhesion					1671:1678	preferential U2OS cell adhesion	1648:1678	preferential U2OS cell adhesion on hydrophobic hydroxyapatite layer compared to hydrophilic surfaces, thereby promising the regeneration of both soft and hard tissues	1648:1813	FDA/PI on the hydrogel portrayed preferential U2OS cell adhesion on hydrophobic hydroxyapatite layer compared to hydrophilic surfaces, thereby promising the regeneration of both soft and hard tissues.
33515570	3	78	dep	transform	492:500	arg1	infrared					502:509	infrared	502:509	transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA)	492:566	Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA) confirmed this associative interaction which resulted in the formation of a hybrid crosslinked network with the BG nanoparticles acting as pseudo crosslink junctions.
33515570	7	79	theme	release	1266:1272	arg1	kinetics					1274:1281	The AmB release kinetics	1258:1281	The AmB release kinetics from the hydrogel	1258:1299	The AmB release kinetics from the hydrogel followed the Fickian mechanism and showed direct proportionality to gel swelling behavior.
33515570	4	80	theme	Scanning	735:742	arg1	Microscopy					753:762	Scanning Electron Microscopy	735:762	Scanning Electron Microscopy (SEM)	735:768	Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Analysis (EDAX) and Transmission Electron Microscopy (TEM) results confirmed uniform mineralization of BG particles, and their synergetic interaction with the network.
33515570	4	80	theme	Scanning	735:742	arg1	SEM					765:767	SEM	765:767	SEM	765:767	Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Analysis (EDAX) and Transmission Electron Microscopy (TEM) results confirmed uniform mineralization of BG particles, and their synergetic interaction with the network.
33515570	0	81	theme	patch	31:35	arg1	hydrogel					59:66	surface patch bound collagen-pectin hydrogel	23:66	surface patch bound collagen-pectin hydrogel	23:66	Bioactivity reinforced surface patch bound collagen-pectin hydrogel.
33515570	1	82	theme	integrative	249:259	arg1	environment					269:279	an integrative 3D cell environment	246:279	an integrative 3D cell environment	246:279	In this report, we discuss the design of a novel collagen/pectin (CP) hybrid composite hydrogel (CPBG) containing in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment.
33515570	2	83	theme	negative	408:415	arg1	charge					417:422	similar net negative charge	396:422	similar net negative charge	396:422	Systematic analysis of the CP sol revealed collagen and pectin molecules interacted regardless of both possessing similar net negative charge through the mechanism of surface patch binding interaction.
33515570	2	84	theme	similar	396:402	arg1	charge					417:422	similar net negative charge	396:422	similar net negative charge	396:422	Systematic analysis of the CP sol revealed collagen and pectin molecules interacted regardless of both possessing similar net negative charge through the mechanism of surface patch binding interaction.
33515570	0	85	theme	collagen-pectin	43:57	arg1	hydrogel					59:66	surface patch bound collagen-pectin hydrogel	23:66	surface patch bound collagen-pectin hydrogel	23:66	Bioactivity reinforced surface patch bound collagen-pectin hydrogel.
33515570	1	86	theme	cell	264:267	arg1	environment					269:279	an integrative 3D cell environment	246:279	an integrative 3D cell environment	246:279	In this report, we discuss the design of a novel collagen/pectin (CP) hybrid composite hydrogel (CPBG) containing in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment.
33515570	4	87	with	mineralization	880:893	arg1	network					954:960	the network	950:960	the network	950:960	Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Analysis (EDAX) and Transmission Electron Microscopy (TEM) results confirmed uniform mineralization of BG particles, and their synergetic interaction with the network.
33515570	2	88	theme	surface	449:455	arg1	interaction					471:481	surface patch binding interaction	449:481	surface patch binding interaction	449:481	Systematic analysis of the CP sol revealed collagen and pectin molecules interacted regardless of both possessing similar net negative charge through the mechanism of surface patch binding interaction.
33515570	10	89	theme	cell	1666:1669	arg1	adhesion					1671:1678	preferential U2OS cell adhesion	1648:1678	preferential U2OS cell adhesion on hydrophobic hydroxyapatite layer compared to hydrophilic surfaces, thereby promising the regeneration of both soft and hard tissues	1648:1813	FDA/PI on the hydrogel portrayed preferential U2OS cell adhesion on hydrophobic hydroxyapatite layer compared to hydrophilic surfaces, thereby promising the regeneration of both soft and hard tissues.
33515570	9	90	theme	cell	1603:1606	arg1	lines					1608:1612	HaCaT cell lines	1597:1612	HaCaT cell lines	1597:1612	Cell viability tests indicated appreciable compatibility of the hydrogel against U2OS and HaCaT cell lines.
33515570	2	91	theme	CP	309:310	arg1	sol					312:314	the CP sol	305:314	the CP sol	305:314	Systematic analysis of the CP sol revealed collagen and pectin molecules interacted regardless of both possessing similar net negative charge through the mechanism of surface patch binding interaction.
33515570	5	92	theme	simulated	1124:1132	arg1	fluid					1139:1143	simulated body fluid	1124:1143	simulated body fluid	1124:1143	The in-vitro bioactivity tests on CPBG indicated the formation of bone-like hydroxyapatite (Ca10(PO4)6(OH)2) microcrystals on its surface after interaction with simulated body fluid.
33515570	7	93	theme	direct	1343:1348	arg1	proportionality					1350:1364	direct proportionality	1343:1364	direct proportionality to gel swelling behavior	1343:1389	The AmB release kinetics from the hydrogel followed the Fickian mechanism and showed direct proportionality to gel swelling behavior.
33515570	3	94	dep	Fourier	484:490	arg1	transform					492:500	transform	492:500	transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA)	492:566	Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA) confirmed this associative interaction which resulted in the formation of a hybrid crosslinked network with the BG nanoparticles acting as pseudo crosslink junctions.
33515570	3	95	theme	crosslinked	651:661	arg1	network					663:669	a hybrid crosslinked network	642:669	a hybrid crosslinked network with the BG nanoparticles acting as pseudo crosslink junctions	642:732	Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA) confirmed this associative interaction which resulted in the formation of a hybrid crosslinked network with the BG nanoparticles acting as pseudo crosslink junctions.
33515570	4	96	theme	Dispersive	778:787	arg1	Analysis					795:802	Energy Dispersive X-Ray Analysis	771:802	Energy Dispersive X-Ray Analysis (EDAX)	771:809	Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Analysis (EDAX) and Transmission Electron Microscopy (TEM) results confirmed uniform mineralization of BG particles, and their synergetic interaction with the network.
33515570	4	96	theme	Dispersive	778:787	arg1	EDAX					805:808	EDAX	805:808	EDAX	805:808	Scanning Electron Microscopy (SEM), Energy Dispersive X-Ray Analysis (EDAX) and Transmission Electron Microscopy (TEM) results confirmed uniform mineralization of BG particles, and their synergetic interaction with the network.
33515570	10	97	theme	hydroxyapatite	1695:1708	arg1	layer					1710:1714	hydrophobic hydroxyapatite layer	1683:1714	hydrophobic hydroxyapatite layer	1683:1714	FDA/PI on the hydrogel portrayed preferential U2OS cell adhesion on hydrophobic hydroxyapatite layer compared to hydrophilic surfaces, thereby promising the regeneration of both soft and hard tissues.
33515570	1	98	theme	glass	213:217	arg1	particles					224:232	in-situ mineralized bioactive glass (BG) particles	183:232	in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment	183:279	In this report, we discuss the design of a novel collagen/pectin (CP) hybrid composite hydrogel (CPBG) containing in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment.
33515570	8	99	theme	Rheological	1392:1402	arg1	analysis					1404:1411	Rheological analysis	1392:1411	Rheological analysis	1392:1411	Rheological analysis revealed the viscoelastic compatibility of CPBG for the mechanical load bearing applications.
33515570	1	100	theme	BG	220:221	arg1	particles					224:232	in-situ mineralized bioactive glass (BG) particles	183:232	in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment	183:279	In this report, we discuss the design of a novel collagen/pectin (CP) hybrid composite hydrogel (CPBG) containing in-situ mineralized bioactive glass (BG) particles to simulate an integrative 3D cell environment.
33305370	2	0	theme	insoluble	429:437	arg1	IRF					454:456	IRF	454:456	IRF	454:456	The husk was fractionated by sequential extraction into four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF).
33305370	2	0	theme	insoluble	429:437	arg1	fiber					447:451	insoluble residue fiber	429:451	insoluble residue fiber (IRF)	429:457	The husk was fractionated by sequential extraction into four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF).
33305370	2	0	theme	insoluble	429:437	arg1	fractions					329:337	four fractions	324:337	four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF)	324:457	The husk was fractionated by sequential extraction into four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF).
33305370	6	1	theme	glucose	1283:1289	arg1	diffusion					1291:1299	retarding the glucose diffusion	1269:1299	retarding the glucose diffusion across a dialysis membrane	1269:1326	W-SF exhibited significantly higher capacities to adsorb glucose (2.408 mmol g-1 at a glucose concentration of 200 mmol L-1 ) and inhibit α-amylase activity (29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1 ), probably caused by the higher viscosity and hydration properties; while Ac-SF, Al-SF and IRF (especially Al-SF) were more effective in retarding the glucose diffusion across a dialysis membrane (34.97-41.67% at 20-30 min), which might be attributed to particle size and specific surface area.
33305370	6	2	dep	effective	1256:1264	arg1	%					1340:1340	34.97-41.67%	1329:1340	34.97-41.67% at 20-30 min	1329:1353	W-SF exhibited significantly higher capacities to adsorb glucose (2.408 mmol g-1 at a glucose concentration of 200 mmol L-1 ) and inhibit α-amylase activity (29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1 ), probably caused by the higher viscosity and hydration properties; while Ac-SF, Al-SF and IRF (especially Al-SF) were more effective in retarding the glucose diffusion across a dialysis membrane (34.97-41.67% at 20-30 min), which might be attributed to particle size and specific surface area.
33305370	4	3	theme	infrared	637:644	arg1	spectra					646:652	infrared spectra	637:652	infrared spectra	637:652	RESULTS Monosaccharide composition and Fourier transform infrared spectra showed that the major component might be pectin for W-SF and Ac-SF, xylan as well as pectin for Al-SF and cellulose for IRF.
33305370	6	4	from	effective	1256:1264	arg1	diffusion					1291:1299	retarding the glucose diffusion	1269:1299	retarding the glucose diffusion across a dialysis membrane	1269:1326	W-SF exhibited significantly higher capacities to adsorb glucose (2.408 mmol g-1 at a glucose concentration of 200 mmol L-1 ) and inhibit α-amylase activity (29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1 ), probably caused by the higher viscosity and hydration properties; while Ac-SF, Al-SF and IRF (especially Al-SF) were more effective in retarding the glucose diffusion across a dialysis membrane (34.97-41.67% at 20-30 min), which might be attributed to particle size and specific surface area.
33305370	1	5	theme	processing	223:232	arg1	byproduct					202:210	a byproduct	200:210	a byproduct of fructus processing	200:232	BACKGROUND Gardenia (Gardenia jasminoides Ellis) husk rich in dietary fiber is a byproduct of fructus processing, and commonly discarded as waste.
33305370	8	6	dep	fiber	1531:1535	arg1	husk					1551:1554	gardenia husk	1542:1554	gardenia husk	1542:1554	CONCLUSIONS Dietary fiber from gardenia husk, especially W-SF, can be used as a potential hypoglycemic ingredient in diabetic functional foods.
33305370	5	7	theme	water-holding	810:822	arg1	capacity					824:831	excellent water-holding capacity	800:831	excellent water-holding capacity	800:831	These fibers offered excellent water-holding capacity and swelling capacity, except that IRF was only slightly swellable in water.
33305370	6	8	dep	activity	1058:1065	arg1	rate					1092:1095	29.48-49.45% inhibition rate	1068:1095	29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1	1068:1129	W-SF exhibited significantly higher capacities to adsorb glucose (2.408 mmol g-1 at a glucose concentration of 200 mmol L-1 ) and inhibit α-amylase activity (29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1 ), probably caused by the higher viscosity and hydration properties; while Ac-SF, Al-SF and IRF (especially Al-SF) were more effective in retarding the glucose diffusion across a dialysis membrane (34.97-41.67% at 20-30 min), which might be attributed to particle size and specific surface area.
33305370	6	9	theme	retarding	1269:1277	arg1	diffusion					1291:1299	retarding the glucose diffusion	1269:1299	retarding the glucose diffusion across a dialysis membrane	1269:1326	W-SF exhibited significantly higher capacities to adsorb glucose (2.408 mmol g-1 at a glucose concentration of 200 mmol L-1 ) and inhibit α-amylase activity (29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1 ), probably caused by the higher viscosity and hydration properties; while Ac-SF, Al-SF and IRF (especially Al-SF) were more effective in retarding the glucose diffusion across a dialysis membrane (34.97-41.67% at 20-30 min), which might be attributed to particle size and specific surface area.
33305370	6	10	from	diffusion	1291:1299	arg1	effective					1256:1264	effective	1256:1264	effective	1256:1264	W-SF exhibited significantly higher capacities to adsorb glucose (2.408 mmol g-1 at a glucose concentration of 200 mmol L-1 ) and inhibit α-amylase activity (29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1 ), probably caused by the higher viscosity and hydration properties; while Ac-SF, Al-SF and IRF (especially Al-SF) were more effective in retarding the glucose diffusion across a dialysis membrane (34.97-41.67% at 20-30 min), which might be attributed to particle size and specific surface area.
33305370	8	11	used	used	1581:1584	arg2	ingredient					1614:1623	a potential hypoglycemic ingredient	1589:1623	a potential hypoglycemic ingredient in diabetic functional foods	1589:1652	CONCLUSIONS Dietary fiber from gardenia husk, especially W-SF, can be used as a potential hypoglycemic ingredient in diabetic functional foods.
33305370	8	11	used	used	1581:1584	arg2	CONCLUSIONS					1511:1521	CONCLUSIONS	1511:1521	CONCLUSIONS Dietary fiber from gardenia husk	1511:1554	CONCLUSIONS Dietary fiber from gardenia husk, especially W-SF, can be used as a potential hypoglycemic ingredient in diabetic functional foods.
33305370	8	11	used	used	1581:1584	arg2	W-SF					1568:1571	W-SF	1568:1571	especially W-SF	1557:1571	CONCLUSIONS Dietary fiber from gardenia husk, especially W-SF, can be used as a potential hypoglycemic ingredient in diabetic functional foods.
33305370	2	12	dep	fractions	329:337	arg1	W-SF					361:364	water-soluble fiber (W-SF)	340:365	water-soluble fiber (W-SF)	340:365	The husk was fractionated by sequential extraction into four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF).
33305370	2	12	dep	fractions	329:337	arg1	IRF					454:456	IRF	454:456	IRF	454:456	The husk was fractionated by sequential extraction into four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF).
33305370	2	12	dep	fractions	329:337	arg1	Ac-SF					388:392	acid-soluble fiber (Ac-SF)	368:393	acid-soluble fiber (Ac-SF)	368:393	The husk was fractionated by sequential extraction into four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF).
33305370	2	12	dep	fractions	329:337	arg1	fractions					329:337	four fractions	324:337	four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF)	324:457	The husk was fractionated by sequential extraction into four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF).
33305370	2	12	dep	fractions	329:337	arg1	fiber					447:451	insoluble residue fiber	429:451	insoluble residue fiber (IRF)	429:457	The husk was fractionated by sequential extraction into four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF).
33305370	2	12	dep	fractions	329:337	arg1	Al-SF					418:422	alkali-soluble fiber (Al-SF)	396:423	alkali-soluble fiber (Al-SF)	396:423	The husk was fractionated by sequential extraction into four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF).
33305370	8	13	theme	hypoglycemic	1601:1612	arg1	ingredient					1614:1623	a potential hypoglycemic ingredient	1589:1623	a potential hypoglycemic ingredient in diabetic functional foods	1589:1652	CONCLUSIONS Dietary fiber from gardenia husk, especially W-SF, can be used as a potential hypoglycemic ingredient in diabetic functional foods.
33305370	8	13	theme	hypoglycemic	1601:1612	arg1	CONCLUSIONS					1511:1521	CONCLUSIONS	1511:1521	CONCLUSIONS Dietary fiber from gardenia husk	1511:1554	CONCLUSIONS Dietary fiber from gardenia husk, especially W-SF, can be used as a potential hypoglycemic ingredient in diabetic functional foods.
33305370	6	14	theme	inhibition	1081:1090	arg1	rate					1092:1095	29.48-49.45% inhibition rate	1068:1095	29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1	1068:1129	W-SF exhibited significantly higher capacities to adsorb glucose (2.408 mmol g-1 at a glucose concentration of 200 mmol L-1 ) and inhibit α-amylase activity (29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1 ), probably caused by the higher viscosity and hydration properties; while Ac-SF, Al-SF and IRF (especially Al-SF) were more effective in retarding the glucose diffusion across a dialysis membrane (34.97-41.67% at 20-30 min), which might be attributed to particle size and specific surface area.
33305370	0	15	dep	in	91:92	arg1	vitro					94:98	vitro	94:98	vitro	94:98	Dietary fibers fractionated from gardenia (Gardenia jasminoides Ellis) husk: structure and in vitro hypoglycemic effect.
33305370	6	16	from	concentration	1004:1016	arg1	2.408 mmol g-1					976:989	2.408 mmol g-1	976:989	2.408 mmol g-1 at a glucose concentration of 200 mmol L-1	976:1032	W-SF exhibited significantly higher capacities to adsorb glucose (2.408 mmol g-1 at a glucose concentration of 200 mmol L-1 ) and inhibit α-amylase activity (29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1 ), probably caused by the higher viscosity and hydration properties; while Ac-SF, Al-SF and IRF (especially Al-SF) were more effective in retarding the glucose diffusion across a dialysis membrane (34.97-41.67% at 20-30 min), which might be attributed to particle size and specific surface area.
33305370	1	17	dep	BACKGROUND	121:130	arg1	husk					170:173	husk	170:173	husk rich in dietary fiber	170:195	BACKGROUND Gardenia (Gardenia jasminoides Ellis) husk rich in dietary fiber is a byproduct of fructus processing, and commonly discarded as waste.
33305370	8	18	theme	Dietary	1523:1529	arg1	fiber					1531:1535	Dietary fiber	1523:1535	Dietary fiber	1523:1535	CONCLUSIONS Dietary fiber from gardenia husk, especially W-SF, can be used as a potential hypoglycemic ingredient in diabetic functional foods.
33305370	6	19	theme	200 mmol L-1	1021:1032	arg1	concentration					1004:1016	a glucose concentration	994:1016	a glucose concentration of 200 mmol L-1	994:1032	W-SF exhibited significantly higher capacities to adsorb glucose (2.408 mmol g-1 at a glucose concentration of 200 mmol L-1 ) and inhibit α-amylase activity (29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1 ), probably caused by the higher viscosity and hydration properties; while Ac-SF, Al-SF and IRF (especially Al-SF) were more effective in retarding the glucose diffusion across a dialysis membrane (34.97-41.67% at 20-30 min), which might be attributed to particle size and specific surface area.
33305370	2	20	theme	fiber	381:385	arg1	fractions					329:337	four fractions	324:337	four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF)	324:457	The husk was fractionated by sequential extraction into four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF).
33305370	2	20	theme	fiber	381:385	arg1	Ac-SF					388:392	acid-soluble fiber (Ac-SF)	368:393	acid-soluble fiber (Ac-SF)	368:393	The husk was fractionated by sequential extraction into four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF).
33305370	4	21	dep	Fourier	619:625	arg1	transform					627:635	transform	627:635	transform infrared spectra	627:652	RESULTS Monosaccharide composition and Fourier transform infrared spectra showed that the major component might be pectin for W-SF and Ac-SF, xylan as well as pectin for Al-SF and cellulose for IRF.
33305370	0	22	theme	in	91:92	arg1	effect					113:118	in vitro hypoglycemic effect	91:118	in vitro hypoglycemic effect	91:118	Dietary fibers fractionated from gardenia (Gardenia jasminoides Ellis) husk: structure and in vitro hypoglycemic effect.
33305370	6	23	theme	hydration	1178:1186	arg1	properties					1188:1197	hydration properties	1178:1197	hydration properties	1178:1197	W-SF exhibited significantly higher capacities to adsorb glucose (2.408 mmol g-1 at a glucose concentration of 200 mmol L-1 ) and inhibit α-amylase activity (29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1 ), probably caused by the higher viscosity and hydration properties; while Ac-SF, Al-SF and IRF (especially Al-SF) were more effective in retarding the glucose diffusion across a dialysis membrane (34.97-41.67% at 20-30 min), which might be attributed to particle size and specific surface area.
33305370	6	24	dep	glucose	967:973	arg1	2.408 mmol g-1					976:989	2.408 mmol g-1	976:989	2.408 mmol g-1 at a glucose concentration of 200 mmol L-1	976:1032	W-SF exhibited significantly higher capacities to adsorb glucose (2.408 mmol g-1 at a glucose concentration of 200 mmol L-1 ) and inhibit α-amylase activity (29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1 ), probably caused by the higher viscosity and hydration properties; while Ac-SF, Al-SF and IRF (especially Al-SF) were more effective in retarding the glucose diffusion across a dialysis membrane (34.97-41.67% at 20-30 min), which might be attributed to particle size and specific surface area.
33305370	7	25	theme	α-amylase	1485:1493	arg1	fluorescence					1469:1480	the intrinsic fluorescence	1455:1480	the intrinsic fluorescence of α-amylase to some degree	1455:1508	All the fibers could quench the intrinsic fluorescence of α-amylase to some degree.
33305370	5	26	from	water	903:907	arg1	swellable					890:898	swellable	890:898	swellable	890:898	These fibers offered excellent water-holding capacity and swelling capacity, except that IRF was only slightly swellable in water.
33305370	6	27	theme	glucose	996:1002	arg1	concentration					1004:1016	a glucose concentration	994:1016	a glucose concentration of 200 mmol L-1	994:1032	W-SF exhibited significantly higher capacities to adsorb glucose (2.408 mmol g-1 at a glucose concentration of 200 mmol L-1 ) and inhibit α-amylase activity (29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1 ), probably caused by the higher viscosity and hydration properties; while Ac-SF, Al-SF and IRF (especially Al-SF) were more effective in retarding the glucose diffusion across a dialysis membrane (34.97-41.67% at 20-30 min), which might be attributed to particle size and specific surface area.
33305370	2	28	theme	alkali-soluble	396:409	arg1	fractions					329:337	four fractions	324:337	four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF)	324:457	The husk was fractionated by sequential extraction into four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF).
33305370	2	28	theme	alkali-soluble	396:409	arg1	Al-SF					418:422	alkali-soluble fiber (Al-SF)	396:423	alkali-soluble fiber (Al-SF)	396:423	The husk was fractionated by sequential extraction into four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF).
33305370	6	29	from	concentration	1102:1114	arg1	rate					1092:1095	29.48-49.45% inhibition rate	1068:1095	29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1	1068:1129	W-SF exhibited significantly higher capacities to adsorb glucose (2.408 mmol g-1 at a glucose concentration of 200 mmol L-1 ) and inhibit α-amylase activity (29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1 ), probably caused by the higher viscosity and hydration properties; while Ac-SF, Al-SF and IRF (especially Al-SF) were more effective in retarding the glucose diffusion across a dialysis membrane (34.97-41.67% at 20-30 min), which might be attributed to particle size and specific surface area.
33305370	0	30	theme	Dietary	0:6	arg1	fibers					8:13	Dietary fibers	0:13	Dietary fibers fractionated from gardenia (Gardenia jasminoides Ellis)	0:69	Dietary fibers fractionated from gardenia (Gardenia jasminoides Ellis) husk: structure and in vitro hypoglycemic effect.
33305370	0	31	dep	husk	71:74	arg1	fibers					8:13	Dietary fibers	0:13	Dietary fibers fractionated from gardenia (Gardenia jasminoides Ellis)	0:69	Dietary fibers fractionated from gardenia (Gardenia jasminoides Ellis) husk: structure and in vitro hypoglycemic effect.
33305370	6	32	theme	surface	1413:1419	arg1	area					1421:1424	specific surface area	1404:1424	specific surface area	1404:1424	W-SF exhibited significantly higher capacities to adsorb glucose (2.408 mmol g-1 at a glucose concentration of 200 mmol L-1 ) and inhibit α-amylase activity (29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1 ), probably caused by the higher viscosity and hydration properties; while Ac-SF, Al-SF and IRF (especially Al-SF) were more effective in retarding the glucose diffusion across a dialysis membrane (34.97-41.67% at 20-30 min), which might be attributed to particle size and specific surface area.
33305370	2	33	theme	fiber	354:358	arg1	W-SF					361:364	water-soluble fiber (W-SF)	340:365	water-soluble fiber (W-SF)	340:365	The husk was fractionated by sequential extraction into four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF).
33305370	2	33	theme	fiber	354:358	arg1	fractions					329:337	four fractions	324:337	four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF)	324:457	The husk was fractionated by sequential extraction into four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF).
33305370	8	34	from	ingredient	1614:1623	arg1	foods					1648:1652	diabetic functional foods	1628:1652	diabetic functional foods	1628:1652	CONCLUSIONS Dietary fiber from gardenia husk, especially W-SF, can be used as a potential hypoglycemic ingredient in diabetic functional foods.
33305370	3	35	theme	study	476:480	arg1	aim					464:466	The aim	460:466	The aim of this study	460:480	The aim of this study was to investigate the differences in structure and in vitro hypoglycemic effect of these fibers.
33305370	3	36	from	differences	505:515	arg1	effect					556:561	in vitro hypoglycemic effect	534:561	in vitro hypoglycemic effect	534:561	The aim of this study was to investigate the differences in structure and in vitro hypoglycemic effect of these fibers.
33305370	3	36	from	differences	505:515	arg1	structure					520:528	structure	520:528	structure	520:528	The aim of this study was to investigate the differences in structure and in vitro hypoglycemic effect of these fibers.
33305370	3	37	dep	in	534:535	arg1	vitro					537:541	vitro	537:541	vitro	537:541	The aim of this study was to investigate the differences in structure and in vitro hypoglycemic effect of these fibers.
33305370	4	38	theme	major	670:674	arg1	component					676:684	the major component	666:684	the major component	666:684	RESULTS Monosaccharide composition and Fourier transform infrared spectra showed that the major component might be pectin for W-SF and Ac-SF, xylan as well as pectin for Al-SF and cellulose for IRF.
33305370	4	38	theme	major	670:674	arg1	pectin					695:700	pectin	695:700	pectin for W-SF and Ac-SF, xylan as well as pectin for Al-SF and cellulose for IRF	695:776	RESULTS Monosaccharide composition and Fourier transform infrared spectra showed that the major component might be pectin for W-SF and Ac-SF, xylan as well as pectin for Al-SF and cellulose for IRF.
33305370	0	39	theme	hypoglycemic	100:111	arg1	effect					113:118	in vitro hypoglycemic effect	91:118	in vitro hypoglycemic effect	91:118	Dietary fibers fractionated from gardenia (Gardenia jasminoides Ellis) husk: structure and in vitro hypoglycemic effect.
33305370	6	40	theme	29.48-49.45	1068:1078	arg1	%					1079:1079	%	1079:1079	%	1079:1079	W-SF exhibited significantly higher capacities to adsorb glucose (2.408 mmol g-1 at a glucose concentration of 200 mmol L-1 ) and inhibit α-amylase activity (29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1 ), probably caused by the higher viscosity and hydration properties; while Ac-SF, Al-SF and IRF (especially Al-SF) were more effective in retarding the glucose diffusion across a dialysis membrane (34.97-41.67% at 20-30 min), which might be attributed to particle size and specific surface area.
33305370	8	41	theme	diabetic	1628:1635	arg1	foods					1648:1652	diabetic functional foods	1628:1652	diabetic functional foods	1628:1652	CONCLUSIONS Dietary fiber from gardenia husk, especially W-SF, can be used as a potential hypoglycemic ingredient in diabetic functional foods.
33305370	1	42	theme	dietary	183:189	arg1	fiber					191:195	dietary fiber	183:195	dietary fiber	183:195	BACKGROUND Gardenia (Gardenia jasminoides Ellis) husk rich in dietary fiber is a byproduct of fructus processing, and commonly discarded as waste.
33305370	8	43	theme	potential	1591:1599	arg1	ingredient					1614:1623	a potential hypoglycemic ingredient	1589:1623	a potential hypoglycemic ingredient in diabetic functional foods	1589:1652	CONCLUSIONS Dietary fiber from gardenia husk, especially W-SF, can be used as a potential hypoglycemic ingredient in diabetic functional foods.
33305370	8	43	theme	potential	1591:1599	arg1	CONCLUSIONS					1511:1521	CONCLUSIONS	1511:1521	CONCLUSIONS Dietary fiber from gardenia husk	1511:1554	CONCLUSIONS Dietary fiber from gardenia husk, especially W-SF, can be used as a potential hypoglycemic ingredient in diabetic functional foods.
33305370	3	44	theme	fibers	572:577	arg1	effect					556:561	in vitro hypoglycemic effect	534:561	in vitro hypoglycemic effect	534:561	The aim of this study was to investigate the differences in structure and in vitro hypoglycemic effect of these fibers.
33305370	3	44	theme	fibers	572:577	arg1	structure					520:528	structure	520:528	structure	520:528	The aim of this study was to investigate the differences in structure and in vitro hypoglycemic effect of these fibers.
33305370	6	45	theme	higher	1157:1162	arg1	viscosity					1164:1172	the higher viscosity	1153:1172	the higher viscosity	1153:1172	W-SF exhibited significantly higher capacities to adsorb glucose (2.408 mmol g-1 at a glucose concentration of 200 mmol L-1 ) and inhibit α-amylase activity (29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1 ), probably caused by the higher viscosity and hydration properties; while Ac-SF, Al-SF and IRF (especially Al-SF) were more effective in retarding the glucose diffusion across a dialysis membrane (34.97-41.67% at 20-30 min), which might be attributed to particle size and specific surface area.
33305370	4	46	theme	Monosaccharide	588:601	arg1	composition					603:613	Monosaccharide composition	588:613	Monosaccharide composition	588:613	RESULTS Monosaccharide composition and Fourier transform infrared spectra showed that the major component might be pectin for W-SF and Ac-SF, xylan as well as pectin for Al-SF and cellulose for IRF.
33305370	2	47	theme	acid-soluble	368:379	arg1	fractions					329:337	four fractions	324:337	four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF)	324:457	The husk was fractionated by sequential extraction into four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF).
33305370	2	47	theme	acid-soluble	368:379	arg1	Ac-SF					388:392	acid-soluble fiber (Ac-SF)	368:393	acid-soluble fiber (Ac-SF)	368:393	The husk was fractionated by sequential extraction into four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF).
33305370	6	48	from	20-30 min	1345:1353	arg1	%					1340:1340	34.97-41.67%	1329:1340	34.97-41.67% at 20-30 min	1329:1353	W-SF exhibited significantly higher capacities to adsorb glucose (2.408 mmol g-1 at a glucose concentration of 200 mmol L-1 ) and inhibit α-amylase activity (29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1 ), probably caused by the higher viscosity and hydration properties; while Ac-SF, Al-SF and IRF (especially Al-SF) were more effective in retarding the glucose diffusion across a dialysis membrane (34.97-41.67% at 20-30 min), which might be attributed to particle size and specific surface area.
33305370	7	49	theme	intrinsic	1459:1467	arg1	fluorescence					1469:1480	the intrinsic fluorescence	1455:1480	the intrinsic fluorescence of α-amylase to some degree	1455:1508	All the fibers could quench the intrinsic fluorescence of α-amylase to some degree.
33305370	1	50	from	fiber	191:195	arg1	rich					175:178	rich	175:178	rich	175:178	BACKGROUND Gardenia (Gardenia jasminoides Ellis) husk rich in dietary fiber is a byproduct of fructus processing, and commonly discarded as waste.
33305370	9	51	theme	Chemical	1673:1680	arg1	Industry					1682:1689	Chemical Industry	1673:1689	Chemical Industry	1673:1689	© 2020 Society of Chemical Industry.
33305370	6	52	theme	dialysis	1310:1317	arg1	membrane					1319:1326	a dialysis membrane	1308:1326	a dialysis membrane	1308:1326	W-SF exhibited significantly higher capacities to adsorb glucose (2.408 mmol g-1 at a glucose concentration of 200 mmol L-1 ) and inhibit α-amylase activity (29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1 ), probably caused by the higher viscosity and hydration properties; while Ac-SF, Al-SF and IRF (especially Al-SF) were more effective in retarding the glucose diffusion across a dialysis membrane (34.97-41.67% at 20-30 min), which might be attributed to particle size and specific surface area.
33305370	8	53	theme	functional	1637:1646	arg1	foods					1648:1652	diabetic functional foods	1628:1652	diabetic functional foods	1628:1652	CONCLUSIONS Dietary fiber from gardenia husk, especially W-SF, can be used as a potential hypoglycemic ingredient in diabetic functional foods.
33305370	2	54	theme	fiber	411:415	arg1	fractions					329:337	four fractions	324:337	four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF)	324:457	The husk was fractionated by sequential extraction into four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF).
33305370	2	54	theme	fiber	411:415	arg1	Al-SF					418:422	alkali-soluble fiber (Al-SF)	396:423	alkali-soluble fiber (Al-SF)	396:423	The husk was fractionated by sequential extraction into four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF).
33305370	8	55	from	fiber	1531:1535	arg1	ingredient					1614:1623	a potential hypoglycemic ingredient	1589:1623	a potential hypoglycemic ingredient in diabetic functional foods	1589:1652	CONCLUSIONS Dietary fiber from gardenia husk, especially W-SF, can be used as a potential hypoglycemic ingredient in diabetic functional foods.
33305370	8	55	from	fiber	1531:1535	arg1	W-SF					1568:1571	W-SF	1568:1571	especially W-SF	1557:1571	CONCLUSIONS Dietary fiber from gardenia husk, especially W-SF, can be used as a potential hypoglycemic ingredient in diabetic functional foods.
33305370	8	55	from	fiber	1531:1535	arg1	CONCLUSIONS					1511:1521	CONCLUSIONS	1511:1521	CONCLUSIONS Dietary fiber from gardenia husk	1511:1554	CONCLUSIONS Dietary fiber from gardenia husk, especially W-SF, can be used as a potential hypoglycemic ingredient in diabetic functional foods.
33305370	6	56	theme	%	1079:1079	arg1	rate					1092:1095	29.48-49.45% inhibition rate	1068:1095	29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1	1068:1129	W-SF exhibited significantly higher capacities to adsorb glucose (2.408 mmol g-1 at a glucose concentration of 200 mmol L-1 ) and inhibit α-amylase activity (29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1 ), probably caused by the higher viscosity and hydration properties; while Ac-SF, Al-SF and IRF (especially Al-SF) were more effective in retarding the glucose diffusion across a dialysis membrane (34.97-41.67% at 20-30 min), which might be attributed to particle size and specific surface area.
33305370	5	57	theme	swelling	837:844	arg1	capacity					846:853	swelling capacity	837:853	swelling capacity	837:853	These fibers offered excellent water-holding capacity and swelling capacity, except that IRF was only slightly swellable in water.
33305370	6	58	theme	particle	1386:1393	arg1	size					1395:1398	particle size	1386:1398	particle size	1386:1398	W-SF exhibited significantly higher capacities to adsorb glucose (2.408 mmol g-1 at a glucose concentration of 200 mmol L-1 ) and inhibit α-amylase activity (29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1 ), probably caused by the higher viscosity and hydration properties; while Ac-SF, Al-SF and IRF (especially Al-SF) were more effective in retarding the glucose diffusion across a dialysis membrane (34.97-41.67% at 20-30 min), which might be attributed to particle size and specific surface area.
33305370	6	59	theme	4-8 mg mL-1	1119:1129	arg1	concentration					1102:1114	a concentration	1100:1114	a concentration of 4-8 mg mL-1	1100:1129	W-SF exhibited significantly higher capacities to adsorb glucose (2.408 mmol g-1 at a glucose concentration of 200 mmol L-1 ) and inhibit α-amylase activity (29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1 ), probably caused by the higher viscosity and hydration properties; while Ac-SF, Al-SF and IRF (especially Al-SF) were more effective in retarding the glucose diffusion across a dialysis membrane (34.97-41.67% at 20-30 min), which might be attributed to particle size and specific surface area.
33305370	2	60	theme	sequential	297:306	arg1	extraction					308:317	sequential extraction	297:317	sequential extraction	297:317	The husk was fractionated by sequential extraction into four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF).
33305370	5	61	dep	offered	792:798	arg1	swellable					890:898	swellable	890:898	swellable	890:898	These fibers offered excellent water-holding capacity and swelling capacity, except that IRF was only slightly swellable in water.
33305370	3	62	theme	hypoglycemic	543:554	arg1	effect					556:561	in vitro hypoglycemic effect	534:561	in vitro hypoglycemic effect	534:561	The aim of this study was to investigate the differences in structure and in vitro hypoglycemic effect of these fibers.
33305370	6	63	theme	specific	1404:1411	arg1	area					1421:1424	specific surface area	1404:1424	specific surface area	1404:1424	W-SF exhibited significantly higher capacities to adsorb glucose (2.408 mmol g-1 at a glucose concentration of 200 mmol L-1 ) and inhibit α-amylase activity (29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1 ), probably caused by the higher viscosity and hydration properties; while Ac-SF, Al-SF and IRF (especially Al-SF) were more effective in retarding the glucose diffusion across a dialysis membrane (34.97-41.67% at 20-30 min), which might be attributed to particle size and specific surface area.
33305370	6	64	theme	α-amylase	1048:1056	arg1	activity					1058:1065	α-amylase activity	1048:1065	α-amylase activity (29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1 )	1048:1131	W-SF exhibited significantly higher capacities to adsorb glucose (2.408 mmol g-1 at a glucose concentration of 200 mmol L-1 ) and inhibit α-amylase activity (29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1 ), probably caused by the higher viscosity and hydration properties; while Ac-SF, Al-SF and IRF (especially Al-SF) were more effective in retarding the glucose diffusion across a dialysis membrane (34.97-41.67% at 20-30 min), which might be attributed to particle size and specific surface area.
33305370	4	65	dep	RESULTS	580:586	arg1	showed					654:659	showed	654:659	showed that the major component might be pectin for W-SF and Ac-SF, xylan as well as pectin for Al-SF and cellulose for IRF	654:776	RESULTS Monosaccharide composition and Fourier transform infrared spectra showed that the major component might be pectin for W-SF and Ac-SF, xylan as well as pectin for Al-SF and cellulose for IRF.
33305370	1	66	from	rich	175:178	arg1	fiber					191:195	dietary fiber	183:195	dietary fiber	183:195	BACKGROUND Gardenia (Gardenia jasminoides Ellis) husk rich in dietary fiber is a byproduct of fructus processing, and commonly discarded as waste.
33305370	2	67	theme	water-soluble	340:352	arg1	W-SF					361:364	water-soluble fiber (W-SF)	340:365	water-soluble fiber (W-SF)	340:365	The husk was fractionated by sequential extraction into four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF).
33305370	2	67	theme	water-soluble	340:352	arg1	fractions					329:337	four fractions	324:337	four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF)	324:457	The husk was fractionated by sequential extraction into four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF).
33305370	8	68	theme	gardenia	1542:1549	arg1	husk					1551:1554	gardenia husk	1542:1554	gardenia husk	1542:1554	CONCLUSIONS Dietary fiber from gardenia husk, especially W-SF, can be used as a potential hypoglycemic ingredient in diabetic functional foods.
33305370	6	69	theme	higher	939:944	arg1	capacities					946:955	significantly higher capacities	925:955	significantly higher capacities to adsorb glucose (2.408 mmol g-1 at a glucose concentration of 200 mmol L-1 ) and inhibit α-amylase activity (29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1 )	925:1131	W-SF exhibited significantly higher capacities to adsorb glucose (2.408 mmol g-1 at a glucose concentration of 200 mmol L-1 ) and inhibit α-amylase activity (29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1 ), probably caused by the higher viscosity and hydration properties; while Ac-SF, Al-SF and IRF (especially Al-SF) were more effective in retarding the glucose diffusion across a dialysis membrane (34.97-41.67% at 20-30 min), which might be attributed to particle size and specific surface area.
33305370	2	70	theme	residue	439:445	arg1	IRF					454:456	IRF	454:456	IRF	454:456	The husk was fractionated by sequential extraction into four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF).
33305370	2	70	theme	residue	439:445	arg1	fiber					447:451	insoluble residue fiber	429:451	insoluble residue fiber (IRF)	429:457	The husk was fractionated by sequential extraction into four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF).
33305370	2	70	theme	residue	439:445	arg1	fractions					329:337	four fractions	324:337	four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF)	324:457	The husk was fractionated by sequential extraction into four fractions: water-soluble fiber (W-SF), acid-soluble fiber (Ac-SF), alkali-soluble fiber (Al-SF) and insoluble residue fiber (IRF).
33305370	3	71	theme	in	534:535	arg1	effect					556:561	in vitro hypoglycemic effect	534:561	in vitro hypoglycemic effect	534:561	The aim of this study was to investigate the differences in structure and in vitro hypoglycemic effect of these fibers.
33305370	6	72	dep	IRF	1223:1225	arg1	Al-SF					1239:1243	Al-SF	1239:1243	Al-SF	1239:1243	W-SF exhibited significantly higher capacities to adsorb glucose (2.408 mmol g-1 at a glucose concentration of 200 mmol L-1 ) and inhibit α-amylase activity (29.48-49.45% inhibition rate at a concentration of 4-8 mg mL-1 ), probably caused by the higher viscosity and hydration properties; while Ac-SF, Al-SF and IRF (especially Al-SF) were more effective in retarding the glucose diffusion across a dialysis membrane (34.97-41.67% at 20-30 min), which might be attributed to particle size and specific surface area.
33305370	5	73	theme	excellent	800:808	arg1	capacity					824:831	excellent water-holding capacity	800:831	excellent water-holding capacity	800:831	These fibers offered excellent water-holding capacity and swelling capacity, except that IRF was only slightly swellable in water.
33305370	5	74	from	swellable	890:898	arg1	water					903:907	water	903:907	water	903:907	These fibers offered excellent water-holding capacity and swelling capacity, except that IRF was only slightly swellable in water.
33305370	1	75	theme	fructus	215:221	arg1	processing					223:232	fructus processing	215:232	fructus processing	215:232	BACKGROUND Gardenia (Gardenia jasminoides Ellis) husk rich in dietary fiber is a byproduct of fructus processing, and commonly discarded as waste.
34138960	2	0	dep	flour	505:509	arg1	plant					512:516	plant	512:516	plant based with high protease inhibitory activity	512:561	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	1	1	theme	enzyme	260:265	arg1	proteolysis					267:277	gastric and pancreatic enzyme proteolysis	237:277	gastric and pancreatic enzyme proteolysis	237:277	BACKGROUND Oral administration of bioactive peptides has potential clinical advantages, but its applicability is limited due to gastric and pancreatic enzyme proteolysis.
34138960	12	2	theme	mg/kg	1739:1743	arg1	dose					1729:1732	a dose	1727:1732	a dose of 7 mg/kg (6.4 BC + 0.6 soy flour)	1727:1768	BC+ soy combination showed the greatest benefit with a dose of 7 mg/kg (6.4 BC + 0.6 soy flour) having the same degree of benefit as using 20 mg/kg BC alone.
34138960	7	3	theme	CT	1208:1209	arg1	exposure					1211:1218	HCl/pepsin and CT exposure	1193:1218	exposure	1211:1218	RESULTS Proliferative activity of BC reduced 61% following HCl/pepsin and CT exposure.
34138960	12	4	theme	mg/kg	1816:1820	arg1	BC					1822:1823	20 mg/kg BC	1813:1823	20 mg/kg BC alone	1813:1829	BC+ soy combination showed the greatest benefit with a dose of 7 mg/kg (6.4 BC + 0.6 soy flour) having the same degree of benefit as using 20 mg/kg BC alone.
34138960	14	5	theme	decreased	2018:2026	arg1	dose					2028:2031	a decreased dose	2016:2031	a decreased dose	2016:2031	Co-packaging of BC with other food products such as soy flour could result in a decreased dose being required, improving cost-effectiveness and patient compliance.
34138960	2	6	theme	protease	534:541	arg1	activity					554:561	high protease inhibitory activity	529:561	high protease inhibitory activity	529:561	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	7	7	theme	HCl/pepsin	1193:1202	arg1	exposure					1211:1218	HCl/pepsin and CT exposure	1193:1218	exposure	1211:1218	RESULTS Proliferative activity of BC reduced 61% following HCl/pepsin and CT exposure.
34138960	14	8	with	Co-packaging	1938:1949	arg1	products					1973:1980	other food products	1962:1980	other food products such as soy flour	1962:1998	Co-packaging of BC with other food products such as soy flour could result in a decreased dose being required, improving cost-effectiveness and patient compliance.
34138960	14	8	with	Co-packaging	1938:1949	arg1	flour					1994:1998	soy flour	1990:1998	soy flour	1990:1998	Co-packaging of BC with other food products such as soy flour could result in a decreased dose being required, improving cost-effectiveness and patient compliance.
34138960	3	9	theme	%	659:659	arg1	wt/wt					661:665	BC+ 10% wt/wt trehalose, stearine, casein or soy	653:700	BC+ 10% wt/wt trehalose, stearine, casein or soy	653:700	DESIGN Samples alone and in combination (BC+ 10% wt/wt trehalose, stearine, casein or soy) were exposed to HCl/pepsin, followed by trypsin and chymotrypsin ("CT").
34138960	3	9	theme	%	659:659	arg1	combination					640:650	combination	640:650	combination (BC+ 10% wt/wt trehalose, stearine, casein or soy)	640:701	DESIGN Samples alone and in combination (BC+ 10% wt/wt trehalose, stearine, casein or soy) were exposed to HCl/pepsin, followed by trypsin and chymotrypsin ("CT").
34138960	12	10	theme	benefit	1796:1802	arg1	degree					1786:1791	the same degree	1777:1791	the same degree of benefit	1777:1802	BC+ soy combination showed the greatest benefit with a dose of 7 mg/kg (6.4 BC + 0.6 soy flour) having the same degree of benefit as using 20 mg/kg BC alone.
34138960	3	11	theme	BC+	653:655	arg1	wt/wt					661:665	BC+ 10% wt/wt trehalose, stearine, casein or soy	653:700	BC+ 10% wt/wt trehalose, stearine, casein or soy	653:700	DESIGN Samples alone and in combination (BC+ 10% wt/wt trehalose, stearine, casein or soy) were exposed to HCl/pepsin, followed by trypsin and chymotrypsin ("CT").
34138960	3	11	theme	BC+	653:655	arg1	combination					640:650	combination	640:650	combination (BC+ 10% wt/wt trehalose, stearine, casein or soy)	640:701	DESIGN Samples alone and in combination (BC+ 10% wt/wt trehalose, stearine, casein or soy) were exposed to HCl/pepsin, followed by trypsin and chymotrypsin ("CT").
34138960	4	12	dep	cells	821:825	arg1	blue					835:838	blue	835:838	blue	835:838	Assessment of proliferation used gastric AGS cells (Alamar blue), IgG function measured bovine IgG anti-E.
34138960	5	13	theme	growth	917:922	arg1	constituents					931:942	growth factor constituents	917:942	growth factor constituents	917:942	coli binding and ELISAs quantified growth factor constituents.
34138960	7	14	dep	RESULTS	1134:1140	arg1	reduced					1171:1177	reduced	1171:1177	reduced 61% following HCl/pepsin and CT exposure	1171:1218	RESULTS Proliferative activity of BC reduced 61% following HCl/pepsin and CT exposure.
34138960	2	15	theme	present	483:489	arg1	protein					475:481	protein	475:481	protein present in BC	475:495	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	13	16	theme	CONCLUSION	1832:1841	arg1	Soy					1843:1845	CONCLUSION Soy	1832:1845	CONCLUSION Soy	1832:1845	CONCLUSION Soy, and to a lesser extent casein, enhanced the biostability of BC against digestive enzymes.
34138960	10	17	theme	beneficial	1554:1563	arg1	effects					1565:1571	beneficial effects	1554:1571	beneficial effects on weight gain, disease activity index, tissue histology and colonic MPO	1554:1644	Rats given BC alone showed beneficial effects on weight gain, disease activity index, tissue histology and colonic MPO.
34138960	1	18	theme	Oral	120:123	arg1	administration					125:138	BACKGROUND Oral administration	109:138	BACKGROUND Oral administration of bioactive peptides	109:160	BACKGROUND Oral administration of bioactive peptides has potential clinical advantages, but its applicability is limited due to gastric and pancreatic enzyme proteolysis.
34138960	7	19	theme	Proliferative	1142:1154	arg1	activity					1156:1163	Proliferative activity	1142:1163	Proliferative activity of BC	1142:1169	RESULTS Proliferative activity of BC reduced 61% following HCl/pepsin and CT exposure.
34138960	6	20	theme	sodium	1038:1043	arg1	sulphate					1045:1052	dextran sodium sulphate	1030:1052	dextran sodium sulphate (DSS, 4% in drinking water, 7 days, test products started 2 days prior to DSS)	1030:1131	In vivo bioassay assessed ability of BC alone or with soy to reduce injury caused by dextran sodium sulphate (DSS, 4% in drinking water, 7 days, test products started 2 days prior to DSS).
34138960	12	21	theme	soy	1759:1761	arg1	flour					1763:1767	6.4 BC + 0.6 soy flour	1746:1767	flour	1763:1767	BC+ soy combination showed the greatest benefit with a dose of 7 mg/kg (6.4 BC + 0.6 soy flour) having the same degree of benefit as using 20 mg/kg BC alone.
34138960	0	22	theme	peptides	61:68	arg1	efficacy					19:26	efficacy	19:26	efficacy of orally administered bioactive peptides	19:68	Methods to improve efficacy of orally administered bioactive peptides using bovine colostrum as an exemplar.
34138960	1	23	theme	peptides	153:160	arg1	administration					125:138	BACKGROUND Oral administration	109:138	BACKGROUND Oral administration of bioactive peptides	109:160	BACKGROUND Oral administration of bioactive peptides has potential clinical advantages, but its applicability is limited due to gastric and pancreatic enzyme proteolysis.
34138960	9	24	theme	protective	1507:1516	arg1	effects					1518:1524	some intermediate protective effects	1489:1524	some intermediate protective effects	1489:1524	Co-packaging with trehalose was ineffective in preventing digestion whereas casein or stearine provided some intermediate protective effects.
34138960	0	25	theme	bovine	76:81	arg1	colostrum					83:91	bovine colostrum	76:91	bovine colostrum	76:91	Methods to improve efficacy of orally administered bioactive peptides using bovine colostrum as an exemplar.
34138960	0	25	theme	bovine	76:81	arg1	exemplar					99:106	exemplar	99:106	exemplar	99:106	Methods to improve efficacy of orally administered bioactive peptides using bovine colostrum as an exemplar.
34138960	8	26	dep	P<0.01	1364:1369	arg1	all					1360:1362	all	1360:1362	all	1360:1362	This was truncated 50% if soy was co-present, and also protected against loss of total IgG, IgG E.coli binding, TGFβ, lactoferrin and EGF (all P<0.01 vs BC alone).
34138960	1	27	theme	potential	166:174	arg1	advantages					185:194	potential clinical advantages	166:194	potential clinical advantages	166:194	BACKGROUND Oral administration of bioactive peptides has potential clinical advantages, but its applicability is limited due to gastric and pancreatic enzyme proteolysis.
34138960	4	28	theme	AGS	817:819	arg1	cells					821:825	gastric AGS cells	809:825	gastric AGS cells (Alamar blue)	809:839	Assessment of proliferation used gastric AGS cells (Alamar blue), IgG function measured bovine IgG anti-E.
34138960	4	28	theme	AGS	817:819	arg1	function					846:853	IgG function	842:853	IgG function measured bovine IgG anti-E	842:880	Assessment of proliferation used gastric AGS cells (Alamar blue), IgG function measured bovine IgG anti-E.
34138960	14	29	theme	other	1962:1966	arg1	products					1973:1980	other food products	1962:1980	other food products such as soy flour	1962:1998	Co-packaging of BC with other food products such as soy flour could result in a decreased dose being required, improving cost-effectiveness and patient compliance.
34138960	14	29	theme	other	1962:1966	arg1	flour					1994:1998	soy flour	1990:1998	soy flour	1990:1998	Co-packaging of BC with other food products such as soy flour could result in a decreased dose being required, improving cost-effectiveness and patient compliance.
34138960	12	30	theme	same	1781:1784	arg1	degree					1786:1791	the same degree	1777:1791	the same degree of benefit	1777:1802	BC+ soy combination showed the greatest benefit with a dose of 7 mg/kg (6.4 BC + 0.6 soy flour) having the same degree of benefit as using 20 mg/kg BC alone.
34138960	14	31	theme	soy	1990:1992	arg1	flour					1994:1998	soy flour	1990:1998	soy flour	1990:1998	Co-packaging of BC with other food products such as soy flour could result in a decreased dose being required, improving cost-effectiveness and patient compliance.
34138960	2	32	theme	immune	374:379	arg1	colostrum					336:344	bovine colostrum	329:344	bovine colostrum (BC)	329:349	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	2	32	theme	immune	374:379	arg1	source					359:364	a rich source	352:364	a rich source of IgG, immune and growth factors	352:398	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	3	33	theme	CT	770:771	arg1	chymotrypsin					755:766	chymotrypsin	755:766	chymotrypsin ("CT")	755:773	DESIGN Samples alone and in combination (BC+ 10% wt/wt trehalose, stearine, casein or soy) were exposed to HCl/pepsin, followed by trypsin and chymotrypsin ("CT").
34138960	3	33	theme	CT	770:771	arg1	"					772:772	"CT"	769:772	"CT"	769:772	DESIGN Samples alone and in combination (BC+ 10% wt/wt trehalose, stearine, casein or soy) were exposed to HCl/pepsin, followed by trypsin and chymotrypsin ("CT").
34138960	2	34	theme	IgG	369:371	arg1	colostrum					336:344	bovine colostrum	329:344	bovine colostrum (BC)	329:349	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	2	34	theme	IgG	369:371	arg1	source					359:364	a rich source	352:364	a rich source of IgG, immune and growth factors	352:398	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	8	35	theme	IgG	1308:1310	arg1	loss					1294:1297	loss	1294:1297	loss of total IgG, IgG E.coli binding, TGFβ, lactoferrin and EGF (all P<0.01 vs BC alone)	1294:1382	This was truncated 50% if soy was co-present, and also protected against loss of total IgG, IgG E.coli binding, TGFβ, lactoferrin and EGF (all P<0.01 vs BC alone).
34138960	8	36	theme	IgG	1313:1315	arg1	binding					1324:1330	IgG E.coli binding	1313:1330	IgG E.coli binding	1313:1330	This was truncated 50% if soy was co-present, and also protected against loss of total IgG, IgG E.coli binding, TGFβ, lactoferrin and EGF (all P<0.01 vs BC alone).
34138960	2	37	theme	growth	385:390	arg1	factors					392:398	growth factors	385:398	growth factors	385:398	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	2	38	dep	casein	467:472	arg1	protein					475:481	protein	475:481	protein present in BC	475:495	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	4	39	theme	bovine	864:869	arg1	IgG					871:873	bovine IgG anti-E	864:880	bovine IgG anti-E	864:880	Assessment of proliferation used gastric AGS cells (Alamar blue), IgG function measured bovine IgG anti-E.
34138960	8	40	theme	binding	1324:1330	arg1	loss					1294:1297	loss	1294:1297	loss of total IgG, IgG E.coli binding, TGFβ, lactoferrin and EGF (all P<0.01 vs BC alone)	1294:1382	This was truncated 50% if soy was co-present, and also protected against loss of total IgG, IgG E.coli binding, TGFβ, lactoferrin and EGF (all P<0.01 vs BC alone).
34138960	10	41	theme	disease	1589:1595	arg1	index					1606:1610	disease activity index	1589:1610	disease activity index	1589:1610	Rats given BC alone showed beneficial effects on weight gain, disease activity index, tissue histology and colonic MPO.
34138960	0	42	theme	administered	38:49	arg1	peptides					61:68	orally administered bioactive peptides	31:68	orally administered bioactive peptides	31:68	Methods to improve efficacy of orally administered bioactive peptides using bovine colostrum as an exemplar.
34138960	5	43	theme	coli	882:885	arg1	binding					887:893	coli binding	882:893	coli binding	882:893	coli binding and ELISAs quantified growth factor constituents.
34138960	6	44	theme	BC	982:983	arg1	ability					971:977	ability	971:977	ability of BC alone or with soy to reduce injury caused by dextran sodium sulphate (DSS, 4% in drinking water, 7 days, test products started 2 days prior to DSS)	971:1131	In vivo bioassay assessed ability of BC alone or with soy to reduce injury caused by dextran sodium sulphate (DSS, 4% in drinking water, 7 days, test products started 2 days prior to DSS).
34138960	8	45	theme	TGFβ	1333:1336	arg1	loss					1294:1297	loss	1294:1297	loss of total IgG, IgG E.coli binding, TGFβ, lactoferrin and EGF (all P<0.01 vs BC alone)	1294:1382	This was truncated 50% if soy was co-present, and also protected against loss of total IgG, IgG E.coli binding, TGFβ, lactoferrin and EGF (all P<0.01 vs BC alone).
34138960	13	46	theme	digestive	1919:1927	arg1	enzymes					1929:1935	digestive enzymes	1919:1935	digestive enzymes	1919:1935	CONCLUSION Soy, and to a lesser extent casein, enhanced the biostability of BC against digestive enzymes.
34138960	8	47	theme	lactoferrin	1339:1349	arg1	loss					1294:1297	loss	1294:1297	loss of total IgG, IgG E.coli binding, TGFβ, lactoferrin and EGF (all P<0.01 vs BC alone)	1294:1382	This was truncated 50% if soy was co-present, and also protected against loss of total IgG, IgG E.coli binding, TGFβ, lactoferrin and EGF (all P<0.01 vs BC alone).
34138960	10	48	theme	tissue	1613:1618	arg1	histology					1620:1628	tissue histology	1613:1628	tissue histology	1613:1628	Rats given BC alone showed beneficial effects on weight gain, disease activity index, tissue histology and colonic MPO.
34138960	1	49	theme	gastric	237:243	arg1	proteolysis					267:277	gastric and pancreatic enzyme proteolysis	237:277	gastric and pancreatic enzyme proteolysis	237:277	BACKGROUND Oral administration of bioactive peptides has potential clinical advantages, but its applicability is limited due to gastric and pancreatic enzyme proteolysis.
34138960	8	50	theme	EGF	1355:1357	arg1	loss					1294:1297	loss	1294:1297	loss of total IgG, IgG E.coli binding, TGFβ, lactoferrin and EGF (all P<0.01 vs BC alone)	1294:1382	This was truncated 50% if soy was co-present, and also protected against loss of total IgG, IgG E.coli binding, TGFβ, lactoferrin and EGF (all P<0.01 vs BC alone).
34138960	6	51	theme	test	1090:1093	arg1	days					1084:1087	7 days	1082:1087	7 days	1082:1087	In vivo bioassay assessed ability of BC alone or with soy to reduce injury caused by dextran sodium sulphate (DSS, 4% in drinking water, 7 days, test products started 2 days prior to DSS).
34138960	6	51	theme	test	1090:1093	arg1	products					1095:1102	test products	1090:1102	test products started 2 days prior to DSS	1090:1130	In vivo bioassay assessed ability of BC alone or with soy to reduce injury caused by dextran sodium sulphate (DSS, 4% in drinking water, 7 days, test products started 2 days prior to DSS).
34138960	1	52	theme	pancreatic	249:258	arg1	proteolysis					267:277	gastric and pancreatic enzyme proteolysis	237:277	gastric and pancreatic enzyme proteolysis	237:277	BACKGROUND Oral administration of bioactive peptides has potential clinical advantages, but its applicability is limited due to gastric and pancreatic enzyme proteolysis.
34138960	6	53	theme	In	945:946	arg1	bioassay					953:960	In vivo bioassay	945:960	In vivo bioassay	945:960	In vivo bioassay assessed ability of BC alone or with soy to reduce injury caused by dextran sodium sulphate (DSS, 4% in drinking water, 7 days, test products started 2 days prior to DSS).
34138960	3	54	theme	DESIGN	612:617	arg1	Samples					619:625	DESIGN Samples	612:625	DESIGN Samples alone and in combination (BC+ 10% wt/wt trehalose, stearine, casein or soy)	612:701	DESIGN Samples alone and in combination (BC+ 10% wt/wt trehalose, stearine, casein or soy) were exposed to HCl/pepsin, followed by trypsin and chymotrypsin ("CT").
34138960	9	55	with	Co-packaging	1385:1396	arg1	trehalose					1403:1411	trehalose	1403:1411	trehalose	1403:1411	Co-packaging with trehalose was ineffective in preventing digestion whereas casein or stearine provided some intermediate protective effects.
34138960	1	56	contain	has	162:164	arg1	administration					125:138	BACKGROUND Oral administration	109:138	BACKGROUND Oral administration of bioactive peptides	109:160	BACKGROUND Oral administration of bioactive peptides has potential clinical advantages, but its applicability is limited due to gastric and pancreatic enzyme proteolysis.
34138960	1	56	contain	has	162:164	arg2	advantages					185:194	potential clinical advantages	166:194	potential clinical advantages	166:194	BACKGROUND Oral administration of bioactive peptides has potential clinical advantages, but its applicability is limited due to gastric and pancreatic enzyme proteolysis.
34138960	1	57	theme	clinical	176:183	arg1	advantages					185:194	potential clinical advantages	166:194	potential clinical advantages	166:194	BACKGROUND Oral administration of bioactive peptides has potential clinical advantages, but its applicability is limited due to gastric and pancreatic enzyme proteolysis.
34138960	2	58	theme	inhibitory	543:552	arg1	activity					554:561	high protease inhibitory activity	529:561	high protease inhibitory activity	529:561	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	12	59	theme	20	1813:1814	arg1	mg/kg					1816:1820	mg/kg	1816:1820	mg/kg	1816:1820	BC+ soy combination showed the greatest benefit with a dose of 7 mg/kg (6.4 BC + 0.6 soy flour) having the same degree of benefit as using 20 mg/kg BC alone.
34138960	6	60	theme	drinking	1066:1073	arg1	water					1075:1079	drinking water	1066:1079	drinking water	1066:1079	In vivo bioassay assessed ability of BC alone or with soy to reduce injury caused by dextran sodium sulphate (DSS, 4% in drinking water, 7 days, test products started 2 days prior to DSS).
34138960	2	61	theme	bovine	329:334	arg1	BC					347:348	BC	347:348	BC	347:348	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	2	61	theme	bovine	329:334	arg1	colostrum					336:344	bovine colostrum	329:344	bovine colostrum (BC)	329:349	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	2	61	theme	bovine	329:334	arg1	source					359:364	a rich source	352:364	a rich source of IgG, immune and growth factors	352:398	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	12	62	theme	greatest	1705:1712	arg1	benefit					1714:1720	the greatest benefit	1701:1720	the greatest benefit	1701:1720	BC+ soy combination showed the greatest benefit with a dose of 7 mg/kg (6.4 BC + 0.6 soy flour) having the same degree of benefit as using 20 mg/kg BC alone.
34138960	2	63	theme	high	529:532	arg1	activity					554:561	high protease inhibitory activity	529:561	high protease inhibitory activity	529:561	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	8	64	theme	truncated	1230:1238	arg1	%					1242:1242	truncated 50%	1230:1242	truncated 50%	1230:1242	This was truncated 50% if soy was co-present, and also protected against loss of total IgG, IgG E.coli binding, TGFβ, lactoferrin and EGF (all P<0.01 vs BC alone).
34138960	5	65	theme	factor	924:929	arg1	constituents					931:942	growth factor constituents	917:942	growth factor constituents	917:942	coli binding and ELISAs quantified growth factor constituents.
34138960	2	66	attach	present	483:489	arg2	protein					475:481	protein	475:481	protein present in BC	475:495	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	2	66	attach	present	483:489	arg1	BC					494:495	BC	494:495	BC	494:495	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	12	67	theme	soy	1678:1680	arg1	combination					1682:1692	BC+ soy combination	1674:1692	BC+ soy combination	1674:1692	BC+ soy combination showed the greatest benefit with a dose of 7 mg/kg (6.4 BC + 0.6 soy flour) having the same degree of benefit as using 20 mg/kg BC alone.
34138960	4	68	theme	proliferation	790:802	arg1	Assessment					776:785	Assessment	776:785	Assessment of proliferation	776:802	Assessment of proliferation used gastric AGS cells (Alamar blue), IgG function measured bovine IgG anti-E.
34138960	1	69	theme	BACKGROUND	109:118	arg1	administration					125:138	BACKGROUND Oral administration	109:138	BACKGROUND Oral administration of bioactive peptides	109:160	BACKGROUND Oral administration of bioactive peptides has potential clinical advantages, but its applicability is limited due to gastric and pancreatic enzyme proteolysis.
34138960	2	70	theme	soy	501:503	arg1	additives					415:423	the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity)	406:562	the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity)	406:562	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	2	70	theme	soy	501:503	arg1	flour					505:509	soy flour	501:509	soy flour (plant based with high protease inhibitory activity)	501:562	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	13	71	dep	Soy	1843:1845	arg1	casein					1871:1876	casein	1871:1876	casein	1871:1876	CONCLUSION Soy, and to a lesser extent casein, enhanced the biostability of BC against digestive enzymes.
34138960	7	72	theme	BC	1168:1169	arg1	activity					1156:1163	Proliferative activity	1142:1163	Proliferative activity of BC	1142:1169	RESULTS Proliferative activity of BC reduced 61% following HCl/pepsin and CT exposure.
34138960	3	73	dep	exposed	708:714	arg1	followed					731:738	followed	731:738	followed by trypsin and chymotrypsin ("CT")	731:773	DESIGN Samples alone and in combination (BC+ 10% wt/wt trehalose, stearine, casein or soy) were exposed to HCl/pepsin, followed by trypsin and chymotrypsin ("CT").
34138960	6	74	dep	sulphate	1045:1052	arg1	days					1084:1087	7 days	1082:1087	7 days	1082:1087	In vivo bioassay assessed ability of BC alone or with soy to reduce injury caused by dextran sodium sulphate (DSS, 4% in drinking water, 7 days, test products started 2 days prior to DSS).
34138960	6	74	dep	sulphate	1045:1052	arg1	DSS					1055:1057	DSS	1055:1057	DSS	1055:1057	In vivo bioassay assessed ability of BC alone or with soy to reduce injury caused by dextran sodium sulphate (DSS, 4% in drinking water, 7 days, test products started 2 days prior to DSS).
34138960	6	74	dep	sulphate	1045:1052	arg1	%					1061:1061	4%	1060:1061	4% in drinking water	1060:1079	In vivo bioassay assessed ability of BC alone or with soy to reduce injury caused by dextran sodium sulphate (DSS, 4% in drinking water, 7 days, test products started 2 days prior to DSS).
34138960	6	75	from	%	1061:1061	arg1	water					1075:1079	drinking water	1066:1079	drinking water	1066:1079	In vivo bioassay assessed ability of BC alone or with soy to reduce injury caused by dextran sodium sulphate (DSS, 4% in drinking water, 7 days, test products started 2 days prior to DSS).
34138960	10	76	theme	weight	1576:1581	arg1	gain					1583:1586	weight gain	1576:1586	weight gain	1576:1586	Rats given BC alone showed beneficial effects on weight gain, disease activity index, tissue histology and colonic MPO.
34138960	1	77	theme	bioactive	143:151	arg1	peptides					153:160	bioactive peptides	143:160	bioactive peptides	143:160	BACKGROUND Oral administration of bioactive peptides has potential clinical advantages, but its applicability is limited due to gastric and pancreatic enzyme proteolysis.
34138960	9	78	theme	intermediate	1494:1505	arg1	effects					1518:1524	some intermediate protective effects	1489:1524	some intermediate protective effects	1489:1524	Co-packaging with trehalose was ineffective in preventing digestion whereas casein or stearine provided some intermediate protective effects.
34138960	6	79	theme	dextran	1030:1036	arg1	sulphate					1045:1052	dextran sodium sulphate	1030:1052	dextran sodium sulphate (DSS, 4% in drinking water, 7 days, test products started 2 days prior to DSS)	1030:1131	In vivo bioassay assessed ability of BC alone or with soy to reduce injury caused by dextran sodium sulphate (DSS, 4% in drinking water, 7 days, test products started 2 days prior to DSS).
34138960	2	80	with	co-packaging	313:324	arg1	additives					415:423	the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity)	406:562	the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity)	406:562	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	2	80	with	co-packaging	313:324	arg1	casein					467:472	casein	467:472	casein (protein present in BC)	467:496	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	2	80	with	co-packaging	313:324	arg1	stearine					451:458	stearine	451:458	stearine (fat)	451:464	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	2	80	with	co-packaging	313:324	arg1	trehalose					425:433	trehalose	425:433	trehalose (carbohydrate)	425:448	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	2	80	with	co-packaging	313:324	arg1	flour					505:509	soy flour	501:509	soy flour (plant based with high protease inhibitory activity)	501:562	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	14	81	theme	food	1968:1971	arg1	products					1973:1980	other food products	1962:1980	other food products such as soy flour	1962:1998	Co-packaging of BC with other food products such as soy flour could result in a decreased dose being required, improving cost-effectiveness and patient compliance.
34138960	14	81	theme	food	1968:1971	arg1	flour					1994:1998	soy flour	1990:1998	soy flour	1990:1998	Co-packaging of BC with other food products such as soy flour could result in a decreased dose being required, improving cost-effectiveness and patient compliance.
34138960	3	82	dep	wt/wt	661:665	arg1	soy					698:700	soy	698:700	soy	698:700	DESIGN Samples alone and in combination (BC+ 10% wt/wt trehalose, stearine, casein or soy) were exposed to HCl/pepsin, followed by trypsin and chymotrypsin ("CT").
34138960	3	82	dep	wt/wt	661:665	arg1	casein					688:693	casein	688:693	casein	688:693	DESIGN Samples alone and in combination (BC+ 10% wt/wt trehalose, stearine, casein or soy) were exposed to HCl/pepsin, followed by trypsin and chymotrypsin ("CT").
34138960	3	82	dep	wt/wt	661:665	arg1	stearine					678:685	stearine	678:685	stearine	678:685	DESIGN Samples alone and in combination (BC+ 10% wt/wt trehalose, stearine, casein or soy) were exposed to HCl/pepsin, followed by trypsin and chymotrypsin ("CT").
34138960	3	82	dep	wt/wt	661:665	arg1	trehalose					667:675	trehalose	667:675	trehalose	667:675	DESIGN Samples alone and in combination (BC+ 10% wt/wt trehalose, stearine, casein or soy) were exposed to HCl/pepsin, followed by trypsin and chymotrypsin ("CT").
34138960	12	83	theme	BC+	1674:1676	arg1	combination					1682:1692	BC+ soy combination	1674:1692	BC+ soy combination	1674:1692	BC+ soy combination showed the greatest benefit with a dose of 7 mg/kg (6.4 BC + 0.6 soy flour) having the same degree of benefit as using 20 mg/kg BC alone.
34138960	4	84	theme	gastric	809:815	arg1	cells					821:825	gastric AGS cells	809:825	gastric AGS cells (Alamar blue)	809:839	Assessment of proliferation used gastric AGS cells (Alamar blue), IgG function measured bovine IgG anti-E.
34138960	4	84	theme	gastric	809:815	arg1	function					846:853	IgG function	842:853	IgG function measured bovine IgG anti-E	842:880	Assessment of proliferation used gastric AGS cells (Alamar blue), IgG function measured bovine IgG anti-E.
34138960	8	85	dep	EGF	1355:1357	arg1	P<0.01					1364:1369	P<0.01	1364:1369	P<0.01	1364:1369	This was truncated 50% if soy was co-present, and also protected against loss of total IgG, IgG E.coli binding, TGFβ, lactoferrin and EGF (all P<0.01 vs BC alone).
34138960	8	85	dep	EGF	1355:1357	arg1	BC					1374:1375	BC	1374:1375	BC	1374:1375	This was truncated 50% if soy was co-present, and also protected against loss of total IgG, IgG E.coli binding, TGFβ, lactoferrin and EGF (all P<0.01 vs BC alone).
34138960	8	86	theme	total	1302:1306	arg1	IgG					1308:1310	total IgG	1302:1310	total IgG	1302:1310	This was truncated 50% if soy was co-present, and also protected against loss of total IgG, IgG E.coli binding, TGFβ, lactoferrin and EGF (all P<0.01 vs BC alone).
34138960	4	87	used	used	804:807	arg2	Assessment					776:785	Assessment	776:785	Assessment of proliferation	776:802	Assessment of proliferation used gastric AGS cells (Alamar blue), IgG function measured bovine IgG anti-E.
34138960	2	88	dep	additives	415:423	arg1	casein					467:472	casein	467:472	casein (protein present in BC)	467:496	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	2	88	dep	additives	415:423	arg1	additives					415:423	the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity)	406:562	the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity)	406:562	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	2	88	dep	additives	415:423	arg1	stearine					451:458	stearine	451:458	stearine (fat)	451:464	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	2	88	dep	additives	415:423	arg1	flour					505:509	soy flour	501:509	soy flour (plant based with high protease inhibitory activity)	501:562	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	2	88	dep	additives	415:423	arg1	fat					461:463	fat	461:463	fat	461:463	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	2	88	dep	additives	415:423	arg1	trehalose					425:433	trehalose	425:433	trehalose (carbohydrate)	425:448	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	2	88	dep	additives	415:423	arg1	carbohydrate					436:447	carbohydrate	436:447	carbohydrate	436:447	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	2	89	theme	food	410:413	arg1	additives					415:423	the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity)	406:562	the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity)	406:562	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	2	89	theme	food	410:413	arg1	casein					467:472	casein	467:472	casein (protein present in BC)	467:496	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	2	89	theme	food	410:413	arg1	stearine					451:458	stearine	451:458	stearine (fat)	451:464	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	2	89	theme	food	410:413	arg1	trehalose					425:433	trehalose	425:433	trehalose (carbohydrate)	425:448	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	2	89	theme	food	410:413	arg1	flour					505:509	soy flour	501:509	soy flour (plant based with high protease inhibitory activity)	501:562	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	2	90	theme	BC	590:591	arg1	stability					577:585	the stability	573:585	the stability of BC	573:591	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	8	91	theme	E.coli	1317:1322	arg1	binding					1324:1330	IgG E.coli binding	1313:1330	IgG E.coli binding	1313:1330	This was truncated 50% if soy was co-present, and also protected against loss of total IgG, IgG E.coli binding, TGFβ, lactoferrin and EGF (all P<0.01 vs BC alone).
34138960	2	92	theme	factors	392:398	arg1	colostrum					336:344	bovine colostrum	329:344	bovine colostrum (BC)	329:349	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	2	92	theme	factors	392:398	arg1	source					359:364	a rich source	352:364	a rich source of IgG, immune and growth factors	352:398	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	13	93	theme	BC	1908:1909	arg1	biostability					1892:1903	the biostability	1888:1903	the biostability of BC against digestive enzymes	1888:1935	CONCLUSION Soy, and to a lesser extent casein, enhanced the biostability of BC against digestive enzymes.
34138960	4	94	theme	IgG	842:844	arg1	cells					821:825	gastric AGS cells	809:825	gastric AGS cells (Alamar blue)	809:839	Assessment of proliferation used gastric AGS cells (Alamar blue), IgG function measured bovine IgG anti-E.
34138960	4	94	theme	IgG	842:844	arg1	function					846:853	IgG function	842:853	IgG function measured bovine IgG anti-E	842:880	Assessment of proliferation used gastric AGS cells (Alamar blue), IgG function measured bovine IgG anti-E.
34138960	12	95	dep	dose	1729:1732	arg1	flour					1763:1767	6.4 BC + 0.6 soy flour	1746:1767	flour	1763:1767	BC+ soy combination showed the greatest benefit with a dose of 7 mg/kg (6.4 BC + 0.6 soy flour) having the same degree of benefit as using 20 mg/kg BC alone.
34138960	12	95	dep	dose	1729:1732	arg1	BC					1750:1751	6.4 BC + 0.6 soy flour	1746:1767	BC	1750:1751	BC+ soy combination showed the greatest benefit with a dose of 7 mg/kg (6.4 BC + 0.6 soy flour) having the same degree of benefit as using 20 mg/kg BC alone.
34138960	10	96	theme	activity	1597:1604	arg1	index					1606:1610	disease activity index	1589:1610	disease activity index	1589:1610	Rats given BC alone showed beneficial effects on weight gain, disease activity index, tissue histology and colonic MPO.
34138960	6	97	dep	In	945:946	arg1	vivo					948:951	vivo	948:951	vivo	948:951	In vivo bioassay assessed ability of BC alone or with soy to reduce injury caused by dextran sodium sulphate (DSS, 4% in drinking water, 7 days, test products started 2 days prior to DSS).
34138960	0	98	theme	bioactive	51:59	arg1	peptides					61:68	orally administered bioactive peptides	31:68	orally administered bioactive peptides	31:68	Methods to improve efficacy of orally administered bioactive peptides using bovine colostrum as an exemplar.
34138960	10	99	theme	colonic	1634:1640	arg1	MPO					1642:1644	colonic MPO	1634:1644	colonic MPO	1634:1644	Rats given BC alone showed beneficial effects on weight gain, disease activity index, tissue histology and colonic MPO.
34138960	14	100	theme	BC	1954:1955	arg1	Co-packaging					1938:1949	Co-packaging	1938:1949	Co-packaging of BC with other food products such as soy flour	1938:1998	Co-packaging of BC with other food products such as soy flour could result in a decreased dose being required, improving cost-effectiveness and patient compliance.
34138960	2	101	from	present	483:489	arg1	BC					494:495	BC	494:495	BC	494:495	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	14	102	theme	patient	2082:2088	arg1	compliance					2090:2099	patient compliance	2082:2099	patient compliance	2082:2099	Co-packaging of BC with other food products such as soy flour could result in a decreased dose being required, improving cost-effectiveness and patient compliance.
34138960	2	103	theme	colostrum	336:344	arg1	co-packaging					313:324	the co-packaging	309:324	the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity)	309:562	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	10	104	from	effects	1565:1571	arg1	index					1606:1610	disease activity index	1589:1610	disease activity index	1589:1610	Rats given BC alone showed beneficial effects on weight gain, disease activity index, tissue histology and colonic MPO.
34138960	10	104	from	effects	1565:1571	arg1	histology					1620:1628	tissue histology	1613:1628	tissue histology	1613:1628	Rats given BC alone showed beneficial effects on weight gain, disease activity index, tissue histology and colonic MPO.
34138960	10	104	from	effects	1565:1571	arg1	MPO					1642:1644	colonic MPO	1634:1644	colonic MPO	1634:1644	Rats given BC alone showed beneficial effects on weight gain, disease activity index, tissue histology and colonic MPO.
34138960	10	104	from	effects	1565:1571	arg1	gain					1583:1586	weight gain	1576:1586	weight gain	1576:1586	Rats given BC alone showed beneficial effects on weight gain, disease activity index, tissue histology and colonic MPO.
34138960	2	105	from	BC	494:495	arg1	present					483:489	present	483:489	present	483:489	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	2	106	theme	rich	354:357	arg1	colostrum					336:344	bovine colostrum	329:344	bovine colostrum (BC)	329:349	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	2	106	theme	rich	354:357	arg1	source					359:364	a rich source	352:364	a rich source of IgG, immune and growth factors	352:398	OBJECTIVE To examine whether the co-packaging of bovine colostrum (BC), a rich source of IgG, immune and growth factors, with the food additives trehalose (carbohydrate), stearine (fat), casein (protein present in BC) or soy flour (plant based with high protease inhibitory activity) enhances the stability of BC against digestion.
34138960	13	107	theme	lesser	1857:1862	arg1	extent					1864:1869	a lesser extent	1855:1869	a lesser extent	1855:1869	CONCLUSION Soy, and to a lesser extent casein, enhanced the biostability of BC against digestive enzymes.
34123172	6	0	with	cellulose	1119:1127	arg1	affinity					1168:1175	low affinity	1164:1175	low affinity	1164:1175	Our results suggest that glycoforms displaying highly correlated motion in their glycosylation sites tend to bind cellulose with high affinity and lignin with low affinity.
34123172	6	0	with	cellulose	1119:1127	arg1	affinity					1139:1146	high affinity	1134:1146	high affinity	1134:1146	Our results suggest that glycoforms displaying highly correlated motion in their glycosylation sites tend to bind cellulose with high affinity and lignin with low affinity.
34123172	2	1	theme	module	375:380	arg1	one					416:418	one	416:418	one	416:418	In this study, we examine the impact of O-glycosylation on the binding selectivity of a model Family 1 carbohydrate-binding module (CBM), which has been shown to be one of the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases.
34123172	2	1	theme	module	375:380	arg1	selectivity					322:332	the binding selectivity	310:332	the binding selectivity	310:332	In this study, we examine the impact of O-glycosylation on the binding selectivity of a model Family 1 carbohydrate-binding module (CBM), which has been shown to be one of the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases.
34123172	2	1	theme	module	375:380	arg1	sub-domains					435:445	the primary sub-domains	423:445	the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases	423:519	In this study, we examine the impact of O-glycosylation on the binding selectivity of a model Family 1 carbohydrate-binding module (CBM), which has been shown to be one of the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases.
34123172	2	2	theme	lignin	478:483	arg1	binding					485:491	non-productive lignin binding	463:491	non-productive lignin binding in multi-modular cellulases	463:519	In this study, we examine the impact of O-glycosylation on the binding selectivity of a model Family 1 carbohydrate-binding module (CBM), which has been shown to be one of the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases.
34123172	2	3	theme	carbohydrate-binding	354:373	arg1	module					375:380	a model Family 1 carbohydrate-binding module	337:380	a model Family 1 carbohydrate-binding module (CBM)	337:386	In this study, we examine the impact of O-glycosylation on the binding selectivity of a model Family 1 carbohydrate-binding module (CBM), which has been shown to be one of the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases.
34123172	2	3	theme	carbohydrate-binding	354:373	arg1	CBM					383:385	CBM	383:385	CBM	383:385	In this study, we examine the impact of O-glycosylation on the binding selectivity of a model Family 1 carbohydrate-binding module (CBM), which has been shown to be one of the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases.
34123172	5	4	theme	glycosylation	984:996	arg1	sites					998:1002	glycosylation sites	984:1002	glycosylation sites	984:1002	In addition, the large set of binding data collected allows us to examine the relationship between binding affinity and the correlation in motion between pairs of glycosylation sites.
34123172	3	5	theme	glycan	572:577	arg1	structure					579:587	glycan structure	572:587	glycan structure	572:587	Specifically, we examine the relationship between glycan structure and the binding specificity of the CBM to cellulose and lignin substrates.
34123172	6	6	theme	low	1164:1166	arg1	affinity					1168:1175	low affinity	1164:1175	low affinity	1164:1175	Our results suggest that glycoforms displaying highly correlated motion in their glycosylation sites tend to bind cellulose with high affinity and lignin with low affinity.
34123172	2	7	theme	O-glycosylation	291:305	arg1	impact					281:286	the impact	277:286	the impact of O-glycosylation on the binding selectivity of a model Family 1 carbohydrate-binding module (CBM), which has been shown to be one of the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases	277:519	In this study, we examine the impact of O-glycosylation on the binding selectivity of a model Family 1 carbohydrate-binding module (CBM), which has been shown to be one of the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases.
34123172	5	8	theme	binding	920:926	arg1	affinity					928:935	binding affinity	920:935	binding affinity	920:935	In addition, the large set of binding data collected allows us to examine the relationship between binding affinity and the correlation in motion between pairs of glycosylation sites.
34123172	7	9	theme	industrial	1313:1322	arg1	enzymes					1324:1330	industrial enzymes	1313:1330	industrial enzymes	1313:1330	Taken together, this work helps lay the groundwork for future exploitation of glycoengineering as a tool to improve the performance of industrial enzymes.
34123172	6	10	theme	glycosylation	1086:1098	arg1	sites					1100:1104	their glycosylation sites	1080:1104	their glycosylation sites	1080:1104	Our results suggest that glycoforms displaying highly correlated motion in their glycosylation sites tend to bind cellulose with high affinity and lignin with low affinity.
34123172	5	11	gly	glycosylation	984:996	arg2	sites					998:1002	glycosylation sites	984:1002	glycosylation sites	984:1002	In addition, the large set of binding data collected allows us to examine the relationship between binding affinity and the correlation in motion between pairs of glycosylation sites.
34123172	1	12	theme	Improved	96:103	arg1	understanding					105:117	Improved understanding	96:117	Improved understanding of the effect of protein glycosylation	96:156	Improved understanding of the effect of protein glycosylation is expected to provide the foundation for the design of protein glycoengineering strategies.
34123172	5	13	theme	binding	851:857	arg1	data					859:862	binding data	851:862	binding data collected	851:872	In addition, the large set of binding data collected allows us to examine the relationship between binding affinity and the correlation in motion between pairs of glycosylation sites.
34123172	0	14	theme	module	21:26	arg1	O-mannosylation					28:42	Carbohydrate-binding module O-mannosylation	0:42	Carbohydrate-binding module O-mannosylation	0:42	Carbohydrate-binding module O-mannosylation alters binding selectivity to cellulose and lignin.
34123172	4	15	theme	binding	749:755	arg1	affinity					757:764	the binding affinity	745:764	the binding affinity	745:764	We find that the glycosylation pattern of the CBM exhibits a strong influence on the binding affinity and the selectivity between both cellulose and lignin.
34123172	5	16	theme	data	859:862	arg1	set					844:846	the large set	834:846	the large set of binding data collected	834:872	In addition, the large set of binding data collected allows us to examine the relationship between binding affinity and the correlation in motion between pairs of glycosylation sites.
34123172	5	16	theme	data	859:862	arg1	data					859:862	binding data	851:862	binding data collected	851:872	In addition, the large set of binding data collected allows us to examine the relationship between binding affinity and the correlation in motion between pairs of glycosylation sites.
34123172	0	17	theme	Carbohydrate-binding	0:19	arg1	O-mannosylation					28:42	Carbohydrate-binding module O-mannosylation	0:42	Carbohydrate-binding module O-mannosylation	0:42	Carbohydrate-binding module O-mannosylation alters binding selectivity to cellulose and lignin.
34123172	7	18	theme	enzymes	1324:1330	arg1	performance					1298:1308	the performance	1294:1308	the performance of industrial enzymes	1294:1330	Taken together, this work helps lay the groundwork for future exploitation of glycoengineering as a tool to improve the performance of industrial enzymes.
34123172	4	19	from	influence	732:740	arg1	affinity					757:764	the binding affinity	745:764	the binding affinity	745:764	We find that the glycosylation pattern of the CBM exhibits a strong influence on the binding affinity and the selectivity between both cellulose and lignin.
34123172	4	19	from	influence	732:740	arg1	selectivity					774:784	the selectivity	770:784	the selectivity between both cellulose and lignin	770:818	We find that the glycosylation pattern of the CBM exhibits a strong influence on the binding affinity and the selectivity between both cellulose and lignin.
34123172	2	20	theme	binding	314:320	arg1	one					416:418	one	416:418	one	416:418	In this study, we examine the impact of O-glycosylation on the binding selectivity of a model Family 1 carbohydrate-binding module (CBM), which has been shown to be one of the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases.
34123172	2	20	theme	binding	314:320	arg1	selectivity					322:332	the binding selectivity	310:332	the binding selectivity	310:332	In this study, we examine the impact of O-glycosylation on the binding selectivity of a model Family 1 carbohydrate-binding module (CBM), which has been shown to be one of the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases.
34123172	2	20	theme	binding	314:320	arg1	sub-domains					435:445	the primary sub-domains	423:445	the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases	423:519	In this study, we examine the impact of O-glycosylation on the binding selectivity of a model Family 1 carbohydrate-binding module (CBM), which has been shown to be one of the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases.
34123172	6	21	theme	correlated	1059:1068	arg1	motion					1070:1075	highly correlated motion	1052:1075	highly correlated motion	1052:1075	Our results suggest that glycoforms displaying highly correlated motion in their glycosylation sites tend to bind cellulose with high affinity and lignin with low affinity.
34123172	2	22	from	binding	485:491	arg1	cellulases					510:519	multi-modular cellulases	496:519	multi-modular cellulases	496:519	In this study, we examine the impact of O-glycosylation on the binding selectivity of a model Family 1 carbohydrate-binding module (CBM), which has been shown to be one of the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases.
34123172	1	23	theme	effect	126:131	arg1	understanding					105:117	Improved understanding	96:117	Improved understanding of the effect of protein glycosylation	96:156	Improved understanding of the effect of protein glycosylation is expected to provide the foundation for the design of protein glycoengineering strategies.
34123172	1	24	theme	protein	214:220	arg1	strategies					239:248	protein glycoengineering strategies	214:248	protein glycoengineering strategies	214:248	Improved understanding of the effect of protein glycosylation is expected to provide the foundation for the design of protein glycoengineering strategies.
34123172	2	25	theme	non-productive	463:476	arg1	binding					485:491	non-productive lignin binding	463:491	non-productive lignin binding in multi-modular cellulases	463:519	In this study, we examine the impact of O-glycosylation on the binding selectivity of a model Family 1 carbohydrate-binding module (CBM), which has been shown to be one of the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases.
34123172	2	26	theme	Family	345:350	arg1	module					375:380	a model Family 1 carbohydrate-binding module	337:380	a model Family 1 carbohydrate-binding module (CBM)	337:386	In this study, we examine the impact of O-glycosylation on the binding selectivity of a model Family 1 carbohydrate-binding module (CBM), which has been shown to be one of the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases.
34123172	2	26	theme	Family	345:350	arg1	CBM					383:385	CBM	383:385	CBM	383:385	In this study, we examine the impact of O-glycosylation on the binding selectivity of a model Family 1 carbohydrate-binding module (CBM), which has been shown to be one of the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases.
34123172	5	27	theme	large	838:842	arg1	set					844:846	the large set	834:846	the large set of binding data collected	834:872	In addition, the large set of binding data collected allows us to examine the relationship between binding affinity and the correlation in motion between pairs of glycosylation sites.
34123172	5	27	theme	large	838:842	arg1	data					859:862	binding data	851:862	binding data collected	851:872	In addition, the large set of binding data collected allows us to examine the relationship between binding affinity and the correlation in motion between pairs of glycosylation sites.
34123172	1	28	theme	glycoengineering	222:237	arg1	strategies					239:248	protein glycoengineering strategies	214:248	protein glycoengineering strategies	214:248	Improved understanding of the effect of protein glycosylation is expected to provide the foundation for the design of protein glycoengineering strategies.
34123172	0	29	theme	binding	51:57	arg1	selectivity					59:69	binding selectivity	51:69	binding selectivity	51:69	Carbohydrate-binding module O-mannosylation alters binding selectivity to cellulose and lignin.
34123172	6	30	with	lignin	1152:1157	arg1	affinity					1168:1175	low affinity	1164:1175	low affinity	1164:1175	Our results suggest that glycoforms displaying highly correlated motion in their glycosylation sites tend to bind cellulose with high affinity and lignin with low affinity.
34123172	6	30	with	lignin	1152:1157	arg1	affinity					1139:1146	high affinity	1134:1146	high affinity	1134:1146	Our results suggest that glycoforms displaying highly correlated motion in their glycosylation sites tend to bind cellulose with high affinity and lignin with low affinity.
34123172	1	31	theme	strategies	239:248	arg1	design					204:209	the design	200:209	the design of protein glycoengineering strategies	200:248	Improved understanding of the effect of protein glycosylation is expected to provide the foundation for the design of protein glycoengineering strategies.
34123172	2	32	theme	responsible	447:457	arg1	sub-domains					435:445	the primary sub-domains	423:445	the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases	423:519	In this study, we examine the impact of O-glycosylation on the binding selectivity of a model Family 1 carbohydrate-binding module (CBM), which has been shown to be one of the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases.
34123172	4	33	gly	glycosylation	681:693	arg1	CBM					710:712	the CBM	706:712	the CBM	706:712	We find that the glycosylation pattern of the CBM exhibits a strong influence on the binding affinity and the selectivity between both cellulose and lignin.
34123172	3	34	theme	lignin	645:650	arg1	substrates					652:661	cellulose and lignin substrates	631:661	cellulose and lignin substrates	631:661	Specifically, we examine the relationship between glycan structure and the binding specificity of the CBM to cellulose and lignin substrates.
34123172	6	35	theme	high	1134:1137	arg1	affinity					1139:1146	high affinity	1134:1146	high affinity	1134:1146	Our results suggest that glycoforms displaying highly correlated motion in their glycosylation sites tend to bind cellulose with high affinity and lignin with low affinity.
34123172	2	36	theme	sub-domains	435:445	arg1	one					416:418	one	416:418	one	416:418	In this study, we examine the impact of O-glycosylation on the binding selectivity of a model Family 1 carbohydrate-binding module (CBM), which has been shown to be one of the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases.
34123172	2	36	theme	sub-domains	435:445	arg1	selectivity					322:332	the binding selectivity	310:332	the binding selectivity	310:332	In this study, we examine the impact of O-glycosylation on the binding selectivity of a model Family 1 carbohydrate-binding module (CBM), which has been shown to be one of the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases.
34123172	2	36	theme	sub-domains	435:445	arg1	sub-domains					435:445	the primary sub-domains	423:445	the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases	423:519	In this study, we examine the impact of O-glycosylation on the binding selectivity of a model Family 1 carbohydrate-binding module (CBM), which has been shown to be one of the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases.
34123172	6	37	gly	glycosylation	1086:1098	arg2	sites					1100:1104	their glycosylation sites	1080:1104	their glycosylation sites	1080:1104	Our results suggest that glycoforms displaying highly correlated motion in their glycosylation sites tend to bind cellulose with high affinity and lignin with low affinity.
34123172	3	38	theme	binding	597:603	arg1	specificity					605:615	the binding specificity	593:615	the binding specificity of the CBM to cellulose and lignin substrates	593:661	Specifically, we examine the relationship between glycan structure and the binding specificity of the CBM to cellulose and lignin substrates.
34123172	1	39	theme	protein	136:142	arg1	glycosylation					144:156	protein glycosylation	136:156	protein glycosylation	136:156	Improved understanding of the effect of protein glycosylation is expected to provide the foundation for the design of protein glycoengineering strategies.
34123172	4	40	theme	strong	725:730	arg1	influence					732:740	a strong influence	723:740	a strong influence on the binding affinity and the selectivity between both cellulose and lignin	723:818	We find that the glycosylation pattern of the CBM exhibits a strong influence on the binding affinity and the selectivity between both cellulose and lignin.
34123172	7	41	theme	glycoengineering	1256:1271	arg1	exploitation					1240:1251	future exploitation	1233:1251	future exploitation of glycoengineering	1233:1271	Taken together, this work helps lay the groundwork for future exploitation of glycoengineering as a tool to improve the performance of industrial enzymes.
34123172	1	42	theme	glycosylation	144:156	arg1	effect					126:131	the effect	122:131	the effect of protein glycosylation	122:156	Improved understanding of the effect of protein glycosylation is expected to provide the foundation for the design of protein glycoengineering strategies.
34123172	2	43	from	impact	281:286	arg1	one					416:418	one	416:418	one	416:418	In this study, we examine the impact of O-glycosylation on the binding selectivity of a model Family 1 carbohydrate-binding module (CBM), which has been shown to be one of the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases.
34123172	2	43	from	impact	281:286	arg1	selectivity					322:332	the binding selectivity	310:332	the binding selectivity	310:332	In this study, we examine the impact of O-glycosylation on the binding selectivity of a model Family 1 carbohydrate-binding module (CBM), which has been shown to be one of the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases.
34123172	2	43	from	impact	281:286	arg1	sub-domains					435:445	the primary sub-domains	423:445	the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases	423:519	In this study, we examine the impact of O-glycosylation on the binding selectivity of a model Family 1 carbohydrate-binding module (CBM), which has been shown to be one of the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases.
34123172	3	44	theme	cellulose	631:639	arg1	substrates					652:661	cellulose and lignin substrates	631:661	cellulose and lignin substrates	631:661	Specifically, we examine the relationship between glycan structure and the binding specificity of the CBM to cellulose and lignin substrates.
34123172	4	45	theme	glycosylation	681:693	arg1	pattern					695:701	the glycosylation pattern	677:701	the glycosylation pattern of the CBM	677:712	We find that the glycosylation pattern of the CBM exhibits a strong influence on the binding affinity and the selectivity between both cellulose and lignin.
34123172	5	46	from	affinity	928:935	arg1	motion					960:965	motion	960:965	motion	960:965	In addition, the large set of binding data collected allows us to examine the relationship between binding affinity and the correlation in motion between pairs of glycosylation sites.
34123172	2	47	theme	model	339:343	arg1	module					375:380	a model Family 1 carbohydrate-binding module	337:380	a model Family 1 carbohydrate-binding module (CBM)	337:386	In this study, we examine the impact of O-glycosylation on the binding selectivity of a model Family 1 carbohydrate-binding module (CBM), which has been shown to be one of the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases.
34123172	2	47	theme	model	339:343	arg1	CBM					383:385	CBM	383:385	CBM	383:385	In this study, we examine the impact of O-glycosylation on the binding selectivity of a model Family 1 carbohydrate-binding module (CBM), which has been shown to be one of the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases.
34123172	2	48	theme	multi-modular	496:508	arg1	cellulases					510:519	multi-modular cellulases	496:519	multi-modular cellulases	496:519	In this study, we examine the impact of O-glycosylation on the binding selectivity of a model Family 1 carbohydrate-binding module (CBM), which has been shown to be one of the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases.
34123172	5	49	theme	sites	998:1002	arg1	pairs					975:979	pairs	975:979	pairs of glycosylation sites	975:1002	In addition, the large set of binding data collected allows us to examine the relationship between binding affinity and the correlation in motion between pairs of glycosylation sites.
34123172	5	50	from	correlation	945:955	arg1	motion					960:965	motion	960:965	motion	960:965	In addition, the large set of binding data collected allows us to examine the relationship between binding affinity and the correlation in motion between pairs of glycosylation sites.
34123172	3	51	theme	CBM	624:626	arg1	specificity					605:615	the binding specificity	593:615	the binding specificity of the CBM to cellulose and lignin substrates	593:661	Specifically, we examine the relationship between glycan structure and the binding specificity of the CBM to cellulose and lignin substrates.
34123172	3	51	theme	CBM	624:626	arg1	structure					579:587	glycan structure	572:587	glycan structure	572:587	Specifically, we examine the relationship between glycan structure and the binding specificity of the CBM to cellulose and lignin substrates.
34123172	2	52	theme	primary	427:433	arg1	sub-domains					435:445	the primary sub-domains	423:445	the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases	423:519	In this study, we examine the impact of O-glycosylation on the binding selectivity of a model Family 1 carbohydrate-binding module (CBM), which has been shown to be one of the primary sub-domains responsible for non-productive lignin binding in multi-modular cellulases.
34123172	7	53	theme	future	1233:1238	arg1	exploitation					1240:1251	future exploitation	1233:1251	future exploitation of glycoengineering	1233:1271	Taken together, this work helps lay the groundwork for future exploitation of glycoengineering as a tool to improve the performance of industrial enzymes.
34123172	4	54	theme	CBM	710:712	arg1	pattern					695:701	the glycosylation pattern	677:701	the glycosylation pattern of the CBM	677:712	We find that the glycosylation pattern of the CBM exhibits a strong influence on the binding affinity and the selectivity between both cellulose and lignin.
32070732	5	0	theme	zeta	796:799	arg1	potential					801:809	zeta potential	796:809	zeta potential	796:809	The optimized formulations were found to have 208 ± 0.01 nm particle size, -32.56 ± 0.03 mV of zeta potential, 86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy.
32070732	6	1	theme	fluorescent	966:976	arg1	accumulation					991:1002	higher fluorescent nanoparticle accumulation	959:1002	higher fluorescent nanoparticle accumulation	959:1002	Florescence study indicates epithelial colon cells parade higher fluorescent nanoparticle accumulation after i.v. administration.
32070732	9	2	theme	cell	1459:1462	arg1	lines					1464:1468	CT26 colon carcinoma cell lines	1438:1468	CT26 colon carcinoma cell lines	1438:1468	From the MTT assay, it can be witnessed that encapsulated IMT-PNPs produces higher & controlled cytotoxicity in CT26 colon carcinoma cell lines.
32070732	1	3	theme	targeting	267:275	arg1	cancer					260:265	colorectal cancer targeting	249:275	colorectal cancer targeting	249:275	The main objective of this research was to develop chitosan based polymeric nanoparticles of Imatinib (IMT-PNPs) for colorectal cancer targeting.
32070732	0	4	theme	colorectal	91:100	arg1	cancer					102:107	colorectal cancer	91:107	colorectal cancer targeting application	91:129	Fabrication and characterization of chitosan-based polymeric nanoparticles of Imatinib for colorectal cancer targeting application.
32070732	4	5	theme	kinetic	523:529	arg1	study					563:567	in-vitro drug deposition study	538:567	in-vitro drug deposition study	538:567	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	4	5	theme	kinetic	523:529	arg1	study					531:535	release kinetic study	515:535	release kinetic study	515:535	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	4	5	theme	kinetic	523:529	arg1	study					588:592	histopathological study	570:592	histopathological study	570:592	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	4	5	theme	kinetic	523:529	arg1	study					674:678	in-vitro cytotoxicity study	652:678	in-vitro cytotoxicity study	652:678	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	4	5	theme	kinetic	523:529	arg1	study					615:619	colon tissue uptake study	595:619	colon tissue uptake study	595:619	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	7	6	theme	IMT-PNPs	1035:1042	arg1	formulations					1044:1055	The IMT-PNPs formulations	1031:1055	The IMT-PNPs formulations	1031:1055	The IMT-PNPs formulations show only 0.46% hemolysis, which indicates the formulation is safer for i.v. administration.
32070732	5	7	theme	208 ± 0.01 nm	747:759	arg1	size					770:773	208 ± 0.01 nm particle size	747:773	208 ± 0.01 nm particle size	747:773	The optimized formulations were found to have 208 ± 0.01 nm particle size, -32.56 ± 0.03 mV of zeta potential, 86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy.
32070732	5	7	theme	208 ± 0.01 nm	747:759	arg1	%					824:824	86.45 ± 0.05%	812:824	86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy	812:898	The optimized formulations were found to have 208 ± 0.01 nm particle size, -32.56 ± 0.03 mV of zeta potential, 86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy.
32070732	5	7	theme	208 ± 0.01 nm	747:759	arg1	-32.56 ± 0.03 mV					776:791	-32.56 ± 0.03 mV	776:791	-32.56 ± 0.03 mV of zeta potential	776:809	The optimized formulations were found to have 208 ± 0.01 nm particle size, -32.56 ± 0.03 mV of zeta potential, 86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy.
32070732	4	8	theme	release	515:521	arg1	study					563:567	in-vitro drug deposition study	538:567	in-vitro drug deposition study	538:567	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	4	8	theme	release	515:521	arg1	study					531:535	release kinetic study	515:535	release kinetic study	515:535	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	4	8	theme	release	515:521	arg1	study					588:592	histopathological study	570:592	histopathological study	570:592	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	4	8	theme	release	515:521	arg1	study					674:678	in-vitro cytotoxicity study	652:678	in-vitro cytotoxicity study	652:678	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	4	8	theme	release	515:521	arg1	study					615:619	colon tissue uptake study	595:619	colon tissue uptake study	595:619	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	9	9	from	cytotoxicity	1422:1433	arg1	lines					1464:1468	CT26 colon carcinoma cell lines	1438:1468	CT26 colon carcinoma cell lines	1438:1468	From the MTT assay, it can be witnessed that encapsulated IMT-PNPs produces higher & controlled cytotoxicity in CT26 colon carcinoma cell lines.
32070732	2	10	theme	central	311:317	arg1	design					329:334	central composite design	311:334	central composite design	311:334	The ionic gelation technique and central composite design was implemented to prepare IMT-PNPs.
32070732	4	11	theme	tissue	601:606	arg1	study					615:619	colon tissue uptake study	595:619	colon tissue uptake study	595:619	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	4	11	theme	tissue	601:606	arg1	study					531:535	release kinetic study	515:535	release kinetic study	515:535	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	5	12	theme	particle	761:768	arg1	size					770:773	208 ± 0.01 nm particle size	747:773	208 ± 0.01 nm particle size	747:773	The optimized formulations were found to have 208 ± 0.01 nm particle size, -32.56 ± 0.03 mV of zeta potential, 86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy.
32070732	5	12	theme	particle	761:768	arg1	%					824:824	86.45 ± 0.05%	812:824	86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy	812:898	The optimized formulations were found to have 208 ± 0.01 nm particle size, -32.56 ± 0.03 mV of zeta potential, 86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy.
32070732	5	12	theme	particle	761:768	arg1	-32.56 ± 0.03 mV					776:791	-32.56 ± 0.03 mV	776:791	-32.56 ± 0.03 mV of zeta potential	776:809	The optimized formulations were found to have 208 ± 0.01 nm particle size, -32.56 ± 0.03 mV of zeta potential, 86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy.
32070732	10	13	theme	research	1492:1499	arg1	outcomes					1475:1482	The outcomes	1471:1482	The outcomes of this research confined, IMT-PNPs	1471:1518	The outcomes of this research confined, IMT-PNPs could be an effective approach in colorectal cancer targeting using i.v. route.
32070732	10	13	theme	research	1492:1499	arg1	approach					1542:1549	an effective approach	1529:1549	an effective approach in colorectal cancer targeting using i.v. route	1529:1597	The outcomes of this research confined, IMT-PNPs could be an effective approach in colorectal cancer targeting using i.v. route.
32070732	7	14	dep	hemolysis	1073:1081	arg1	%					1071:1071	%	1071:1071	%	1071:1071	The IMT-PNPs formulations show only 0.46% hemolysis, which indicates the formulation is safer for i.v. administration.
32070732	4	15	theme	colon	595:599	arg1	study					615:619	colon tissue uptake study	595:619	colon tissue uptake study	595:619	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	4	15	theme	colon	595:599	arg1	study					531:535	release kinetic study	515:535	release kinetic study	515:535	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	5	16	theme	potential	801:809	arg1	size					770:773	208 ± 0.01 nm particle size	747:773	208 ± 0.01 nm particle size	747:773	The optimized formulations were found to have 208 ± 0.01 nm particle size, -32.56 ± 0.03 mV of zeta potential, 86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy.
32070732	5	16	theme	potential	801:809	arg1	-32.56 ± 0.03 mV					776:791	-32.56 ± 0.03 mV	776:791	-32.56 ± 0.03 mV of zeta potential	776:809	The optimized formulations were found to have 208 ± 0.01 nm particle size, -32.56 ± 0.03 mV of zeta potential, 86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy.
32070732	6	17	theme	colon	940:944	arg1	cells					946:950	epithelial colon cells	929:950	epithelial colon cells	929:950	Florescence study indicates epithelial colon cells parade higher fluorescent nanoparticle accumulation after i.v. administration.
32070732	5	18	dep	%	824:824	arg1	efficacy					891:898	in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy	826:898	86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy	812:898	The optimized formulations were found to have 208 ± 0.01 nm particle size, -32.56 ± 0.03 mV of zeta potential, 86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy.
32070732	5	19	theme	%	873:873	arg1	efficacy					891:898	in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy	826:898	86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy	812:898	The optimized formulations were found to have 208 ± 0.01 nm particle size, -32.56 ± 0.03 mV of zeta potential, 86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy.
32070732	8	20	theme	final	1261:1265	arg1	formulation					1267:1277	the final formulation	1257:1277	the final formulation	1257:1277	In histopathological evaluation, the final formulations show no sign of damage in tissues, which indicates the final formulation can be safely administered through i.v. route.
32070732	4	21	theme	&	680:680	arg1	studies					692:698	& stability studies	680:698	& stability studies	680:698	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	5	22	theme	in-vitro	826:833	arg1	efficacy					891:898	in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy	826:898	86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy	812:898	The optimized formulations were found to have 208 ± 0.01 nm particle size, -32.56 ± 0.03 mV of zeta potential, 86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy.
32070732	4	23	theme	drug	498:501	arg1	release					503:509	in-vitro drug release	489:509	in-vitro drug release	489:509	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	2	24	theme	gelation	288:295	arg1	technique					297:305	The ionic gelation technique	278:305	The ionic gelation technique	278:305	The ionic gelation technique and central composite design was implemented to prepare IMT-PNPs.
32070732	1	25	theme	based	192:196	arg1	nanoparticles					208:220	chitosan based polymeric nanoparticles	183:220	chitosan based polymeric nanoparticles of Imatinib (IMT-PNPs) for colorectal cancer targeting	183:275	The main objective of this research was to develop chitosan based polymeric nanoparticles of Imatinib (IMT-PNPs) for colorectal cancer targeting.
32070732	5	26	theme	cumulative	835:844	arg1	efficacy					891:898	in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy	826:898	86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy	812:898	The optimized formulations were found to have 208 ± 0.01 nm particle size, -32.56 ± 0.03 mV of zeta potential, 86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy.
32070732	4	27	theme	in-vitro	489:496	arg1	release					503:509	in-vitro drug release	489:509	in-vitro drug release	489:509	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	9	28	theme	encapsulated	1371:1382	arg1	IMT-PNPs					1384:1391	encapsulated IMT-PNPs	1371:1391	encapsulated IMT-PNPs	1371:1391	From the MTT assay, it can be witnessed that encapsulated IMT-PNPs produces higher & controlled cytotoxicity in CT26 colon carcinoma cell lines.
32070732	1	29	theme	polymeric	198:206	arg1	nanoparticles					208:220	chitosan based polymeric nanoparticles	183:220	chitosan based polymeric nanoparticles of Imatinib (IMT-PNPs) for colorectal cancer targeting	183:275	The main objective of this research was to develop chitosan based polymeric nanoparticles of Imatinib (IMT-PNPs) for colorectal cancer targeting.
32070732	5	30	theme	drug	846:849	arg1	efficacy					891:898	in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy	826:898	86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy	812:898	The optimized formulations were found to have 208 ± 0.01 nm particle size, -32.56 ± 0.03 mV of zeta potential, 86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy.
32070732	5	31	contain	have	742:745	arg1	formulations					715:726	The optimized formulations	701:726	The optimized formulations	701:726	The optimized formulations were found to have 208 ± 0.01 nm particle size, -32.56 ± 0.03 mV of zeta potential, 86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy.
32070732	5	31	contain	have	742:745	arg2	%					824:824	86.45 ± 0.05%	812:824	86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy	812:898	The optimized formulations were found to have 208 ± 0.01 nm particle size, -32.56 ± 0.03 mV of zeta potential, 86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy.
32070732	5	31	contain	have	742:745	arg2	-32.56 ± 0.03 mV					776:791	-32.56 ± 0.03 mV	776:791	-32.56 ± 0.03 mV of zeta potential	776:809	The optimized formulations were found to have 208 ± 0.01 nm particle size, -32.56 ± 0.03 mV of zeta potential, 86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy.
32070732	5	31	contain	have	742:745	arg2	size					770:773	208 ± 0.01 nm particle size	747:773	208 ± 0.01 nm particle size	747:773	The optimized formulations were found to have 208 ± 0.01 nm particle size, -32.56 ± 0.03 mV of zeta potential, 86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy.
32070732	8	32	theme	i.v.	1314:1317	arg1	route					1319:1323	i.v. route	1314:1323	i.v. route	1314:1323	In histopathological evaluation, the final formulations show no sign of damage in tissues, which indicates the final formulation can be safely administered through i.v. route.
32070732	9	33	theme	CT26	1438:1441	arg1	lines					1464:1468	CT26 colon carcinoma cell lines	1438:1468	CT26 colon carcinoma cell lines	1438:1468	From the MTT assay, it can be witnessed that encapsulated IMT-PNPs produces higher & controlled cytotoxicity in CT26 colon carcinoma cell lines.
32070732	10	34	theme	effective	1532:1540	arg1	outcomes					1475:1482	The outcomes	1471:1482	The outcomes of this research confined, IMT-PNPs	1471:1518	The outcomes of this research confined, IMT-PNPs could be an effective approach in colorectal cancer targeting using i.v. route.
32070732	10	34	theme	effective	1532:1540	arg1	approach					1542:1549	an effective approach	1529:1549	an effective approach in colorectal cancer targeting using i.v. route	1529:1597	The outcomes of this research confined, IMT-PNPs could be an effective approach in colorectal cancer targeting using i.v. route.
32070732	8	35	theme	damage	1222:1227	arg1	sign					1214:1217	no sign	1211:1217	no sign of damage	1211:1227	In histopathological evaluation, the final formulations show no sign of damage in tissues, which indicates the final formulation can be safely administered through i.v. route.
32070732	4	36	theme	stability	682:690	arg1	studies					692:698	& stability studies	680:698	& stability studies	680:698	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	5	37	theme	release	851:857	arg1	efficacy					891:898	in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy	826:898	86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy	812:898	The optimized formulations were found to have 208 ± 0.01 nm particle size, -32.56 ± 0.03 mV of zeta potential, 86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy.
32070732	4	38	theme	deposition	552:561	arg1	study					563:567	in-vitro drug deposition study	538:567	in-vitro drug deposition study	538:567	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	4	38	theme	deposition	552:561	arg1	study					531:535	release kinetic study	515:535	release kinetic study	515:535	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	8	39	theme	histopathological	1153:1169	arg1	evaluation					1171:1180	histopathological evaluation	1153:1180	histopathological evaluation	1153:1180	In histopathological evaluation, the final formulations show no sign of damage in tissues, which indicates the final formulation can be safely administered through i.v. route.
32070732	9	40	theme	colon	1443:1447	arg1	lines					1464:1468	CT26 colon carcinoma cell lines	1438:1468	CT26 colon carcinoma cell lines	1438:1468	From the MTT assay, it can be witnessed that encapsulated IMT-PNPs produces higher & controlled cytotoxicity in CT26 colon carcinoma cell lines.
32070732	5	41	theme	68.52 ± 0.01	861:872	arg1	efficacy					891:898	in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy	826:898	86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy	812:898	The optimized formulations were found to have 208 ± 0.01 nm particle size, -32.56 ± 0.03 mV of zeta potential, 86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy.
32070732	3	42	theme	F10	392:394	arg1	formulation					396:406	F10 formulation	392:406	F10 formulation	392:406	Out of 21 batches, F10 formulation was found to be optimized.
32070732	4	43	theme	histopathological	570:586	arg1	study					588:592	histopathological study	570:592	histopathological study	570:592	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	4	43	theme	histopathological	570:586	arg1	study					531:535	release kinetic study	515:535	release kinetic study	515:535	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	10	44	theme	colorectal	1554:1563	arg1	cancer					1565:1570	colorectal cancer targeting	1554:1580	colorectal cancer targeting using i.v. route	1554:1597	The outcomes of this research confined, IMT-PNPs could be an effective approach in colorectal cancer targeting using i.v. route.
32070732	2	45	theme	composite	319:327	arg1	design					329:334	central composite design	311:334	central composite design	311:334	The ionic gelation technique and central composite design was implemented to prepare IMT-PNPs.
32070732	1	46	theme	Imatinib	225:232	arg1	nanoparticles					208:220	chitosan based polymeric nanoparticles	183:220	chitosan based polymeric nanoparticles of Imatinib (IMT-PNPs) for colorectal cancer targeting	183:275	The main objective of this research was to develop chitosan based polymeric nanoparticles of Imatinib (IMT-PNPs) for colorectal cancer targeting.
32070732	0	47	theme	polymeric	51:59	arg1	nanoparticles					61:73	chitosan-based polymeric nanoparticles	36:73	chitosan-based polymeric nanoparticles of Imatinib	36:85	Fabrication and characterization of chitosan-based polymeric nanoparticles of Imatinib for colorectal cancer targeting application.
32070732	4	48	dep	morphology	477:486	arg1	studies					692:698	& stability studies	680:698	& stability studies	680:698	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	5	49	theme	drug	875:878	arg1	efficacy					891:898	in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy	826:898	86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy	812:898	The optimized formulations were found to have 208 ± 0.01 nm particle size, -32.56 ± 0.03 mV of zeta potential, 86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy.
32070732	7	50	dep	%	1071:1071	arg1	0.46					1067:1070	0.46	1067:1070	0.46	1067:1070	The IMT-PNPs formulations show only 0.46% hemolysis, which indicates the formulation is safer for i.v. administration.
32070732	4	51	theme	cytotoxicity	661:672	arg1	study					531:535	release kinetic study	515:535	release kinetic study	515:535	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	4	51	theme	cytotoxicity	661:672	arg1	study					674:678	in-vitro cytotoxicity study	652:678	in-vitro cytotoxicity study	652:678	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	0	52	theme	chitosan-based	36:49	arg1	nanoparticles					61:73	chitosan-based polymeric nanoparticles	36:73	chitosan-based polymeric nanoparticles of Imatinib	36:85	Fabrication and characterization of chitosan-based polymeric nanoparticles of Imatinib for colorectal cancer targeting application.
32070732	7	53	theme	i.v.	1129:1132	arg1	administration					1134:1147	i.v. administration	1129:1147	i.v. administration	1129:1147	The IMT-PNPs formulations show only 0.46% hemolysis, which indicates the formulation is safer for i.v. administration.
32070732	4	54	theme	surface	469:475	arg1	morphology					477:486	surface morphology	469:486	surface morphology	469:486	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	4	55	theme	fluorescence	627:638	arg1	microscopy					640:649	fluorescence microscopy	627:649	fluorescence microscopy	627:649	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	4	56	theme	in-vitro	652:659	arg1	study					531:535	release kinetic study	515:535	release kinetic study	515:535	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	4	56	theme	in-vitro	652:659	arg1	study					674:678	in-vitro cytotoxicity study	652:678	in-vitro cytotoxicity study	652:678	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	6	57	theme	i.v.	1010:1013	arg1	administration					1015:1028	i.v. administration	1010:1028	i.v. administration	1010:1028	Florescence study indicates epithelial colon cells parade higher fluorescent nanoparticle accumulation after i.v. administration.
32070732	8	58	theme	final	1187:1191	arg1	formulations					1193:1204	the final formulations	1183:1204	the final formulations	1183:1204	In histopathological evaluation, the final formulations show no sign of damage in tissues, which indicates the final formulation can be safely administered through i.v. route.
32070732	9	59	theme	higher	1402:1407	arg1	cytotoxicity					1422:1433	higher & controlled cytotoxicity	1402:1433	higher & controlled cytotoxicity in CT26 colon carcinoma cell lines	1402:1468	From the MTT assay, it can be witnessed that encapsulated IMT-PNPs produces higher & controlled cytotoxicity in CT26 colon carcinoma cell lines.
32070732	6	60	theme	epithelial	929:938	arg1	cells					946:950	epithelial colon cells	929:950	epithelial colon cells	929:950	Florescence study indicates epithelial colon cells parade higher fluorescent nanoparticle accumulation after i.v. administration.
32070732	1	61	theme	main	136:139	arg1	objective					141:149	The main objective	132:149	The main objective of this research	132:166	The main objective of this research was to develop chitosan based polymeric nanoparticles of Imatinib (IMT-PNPs) for colorectal cancer targeting.
32070732	0	62	theme	nanoparticles	61:73	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Fabrication and characterization of chitosan-based polymeric nanoparticles of Imatinib for colorectal cancer targeting application.
32070732	0	62	theme	nanoparticles	61:73	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication and characterization of chitosan-based polymeric nanoparticles of Imatinib for colorectal cancer targeting application.
32070732	4	63	theme	drug	547:550	arg1	study					563:567	in-vitro drug deposition study	538:567	in-vitro drug deposition study	538:567	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	4	63	theme	drug	547:550	arg1	study					531:535	release kinetic study	515:535	release kinetic study	515:535	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	10	64	theme	i.v.	1588:1591	arg1	route					1593:1597	i.v. route	1588:1597	i.v. route	1588:1597	The outcomes of this research confined, IMT-PNPs could be an effective approach in colorectal cancer targeting using i.v. route.
32070732	6	65	theme	higher	959:964	arg1	accumulation					991:1002	higher fluorescent nanoparticle accumulation	959:1002	higher fluorescent nanoparticle accumulation	959:1002	Florescence study indicates epithelial colon cells parade higher fluorescent nanoparticle accumulation after i.v. administration.
32070732	1	66	dep	based	192:196	arg1	chitosan					183:190	chitosan	183:190	chitosan	183:190	The main objective of this research was to develop chitosan based polymeric nanoparticles of Imatinib (IMT-PNPs) for colorectal cancer targeting.
32070732	4	67	theme	in-vitro	538:545	arg1	study					563:567	in-vitro drug deposition study	538:567	in-vitro drug deposition study	538:567	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	4	67	theme	in-vitro	538:545	arg1	study					531:535	release kinetic study	515:535	release kinetic study	515:535	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	5	68	theme	optimized	705:713	arg1	formulations					715:726	The optimized formulations	701:726	The optimized formulations	701:726	The optimized formulations were found to have 208 ± 0.01 nm particle size, -32.56 ± 0.03 mV of zeta potential, 86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy.
32070732	2	69	theme	ionic	282:286	arg1	technique					297:305	The ionic gelation technique	278:305	The ionic gelation technique	278:305	The ionic gelation technique and central composite design was implemented to prepare IMT-PNPs.
32070732	9	70	theme	&	1409:1409	arg1	cytotoxicity					1422:1433	higher & controlled cytotoxicity	1402:1433	higher & controlled cytotoxicity in CT26 colon carcinoma cell lines	1402:1468	From the MTT assay, it can be witnessed that encapsulated IMT-PNPs produces higher & controlled cytotoxicity in CT26 colon carcinoma cell lines.
32070732	9	71	theme	carcinoma	1449:1457	arg1	lines					1464:1468	CT26 colon carcinoma cell lines	1438:1468	CT26 colon carcinoma cell lines	1438:1468	From the MTT assay, it can be witnessed that encapsulated IMT-PNPs produces higher & controlled cytotoxicity in CT26 colon carcinoma cell lines.
32070732	10	72	theme	targeting	1572:1580	arg1	cancer					1565:1570	colorectal cancer targeting	1554:1580	colorectal cancer targeting using i.v. route	1554:1597	The outcomes of this research confined, IMT-PNPs could be an effective approach in colorectal cancer targeting using i.v. route.
32070732	9	73	theme	MTT	1335:1337	arg1	assay					1339:1343	the MTT assay	1331:1343	the MTT assay	1331:1343	From the MTT assay, it can be witnessed that encapsulated IMT-PNPs produces higher & controlled cytotoxicity in CT26 colon carcinoma cell lines.
32070732	4	74	theme	uptake	608:613	arg1	study					615:619	colon tissue uptake study	595:619	colon tissue uptake study	595:619	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	4	74	theme	uptake	608:613	arg1	study					531:535	release kinetic study	515:535	release kinetic study	515:535	The F10 was further evaluated for surface morphology, in-vitro drug release and release kinetic study, in-vitro drug deposition study, histopathological study, colon tissue uptake study using fluorescence microscopy, in-vitro cytotoxicity study & stability studies.
32070732	6	75	theme	Florescence	901:911	arg1	study					913:917	Florescence study	901:917	Florescence study	901:917	Florescence study indicates epithelial colon cells parade higher fluorescent nanoparticle accumulation after i.v. administration.
32070732	5	76	theme	entrapment	880:889	arg1	efficacy					891:898	in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy	826:898	86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy	812:898	The optimized formulations were found to have 208 ± 0.01 nm particle size, -32.56 ± 0.03 mV of zeta potential, 86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy.
32070732	10	77	from	approach	1542:1549	arg1	cancer					1565:1570	colorectal cancer targeting	1554:1580	colorectal cancer targeting using i.v. route	1554:1597	The outcomes of this research confined, IMT-PNPs could be an effective approach in colorectal cancer targeting using i.v. route.
32070732	1	78	theme	research	159:166	arg1	objective					141:149	The main objective	132:149	The main objective of this research	132:166	The main objective of this research was to develop chitosan based polymeric nanoparticles of Imatinib (IMT-PNPs) for colorectal cancer targeting.
32070732	9	79	theme	controlled	1411:1420	arg1	cytotoxicity					1422:1433	higher & controlled cytotoxicity	1402:1433	higher & controlled cytotoxicity in CT26 colon carcinoma cell lines	1402:1468	From the MTT assay, it can be witnessed that encapsulated IMT-PNPs produces higher & controlled cytotoxicity in CT26 colon carcinoma cell lines.
32070732	1	80	theme	colorectal	249:258	arg1	cancer					260:265	colorectal cancer targeting	249:275	colorectal cancer targeting	249:275	The main objective of this research was to develop chitosan based polymeric nanoparticles of Imatinib (IMT-PNPs) for colorectal cancer targeting.
32070732	0	81	theme	Imatinib	78:85	arg1	nanoparticles					61:73	chitosan-based polymeric nanoparticles	36:73	chitosan-based polymeric nanoparticles of Imatinib	36:85	Fabrication and characterization of chitosan-based polymeric nanoparticles of Imatinib for colorectal cancer targeting application.
32070732	6	82	theme	nanoparticle	978:989	arg1	accumulation					991:1002	higher fluorescent nanoparticle accumulation	959:1002	higher fluorescent nanoparticle accumulation	959:1002	Florescence study indicates epithelial colon cells parade higher fluorescent nanoparticle accumulation after i.v. administration.
32070732	5	83	theme	&	859:859	arg1	efficacy					891:898	in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy	826:898	86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy	812:898	The optimized formulations were found to have 208 ± 0.01 nm particle size, -32.56 ± 0.03 mV of zeta potential, 86.45 ± 0.05% in-vitro cumulative drug release & 68.52 ± 0.01% drug entrapment efficacy.
33755430	0	0	theme	Wood	85:88	arg1	Materials					109:117	Highly Anisotropic Wood Hydrogel Composite Materials	66:117	Highly Anisotropic Wood Hydrogel Composite Materials with Mechanical Matching and Buckling Buffering	66:165	Overall Structure Construction of an Intervertebral Disk Based on Highly Anisotropic Wood Hydrogel Composite Materials with Mechanical Matching and Buckling Buffering.
33755430	0	1	from	Construction	18:29	arg1	Materials					109:117	Highly Anisotropic Wood Hydrogel Composite Materials	66:117	Highly Anisotropic Wood Hydrogel Composite Materials with Mechanical Matching and Buckling Buffering	66:165	Overall Structure Construction of an Intervertebral Disk Based on Highly Anisotropic Wood Hydrogel Composite Materials with Mechanical Matching and Buckling Buffering.
33755430	4	2	theme	wood	897:900	arg1	AF					945:946	AF	945:946	AF	945:946	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	2	theme	wood	897:900	arg1	fibrosus					935:942	anisotropic wood cellulose hydrogel-based annulus fibrosus	885:942	anisotropic wood cellulose hydrogel-based annulus fibrosus (AF)	885:947	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	1	3	theme	Natural	168:174	arg1	IVDs					198:201	IVDs	198:201	IVDs	198:201	Natural intervertebral disks (IVDs) exhibit distinctive anisotropic mechanical support and dissipation performances due to their well-developed special microstructures.
33755430	1	3	theme	Natural	168:174	arg1	disks					191:195	Natural intervertebral disks	168:195	Natural intervertebral disks (IVDs)	168:202	Natural intervertebral disks (IVDs) exhibit distinctive anisotropic mechanical support and dissipation performances due to their well-developed special microstructures.
33755430	5	4	theme	good	1077:1080	arg1	biocompatibility					1082:1097	good biocompatibility	1077:1097	good biocompatibility	1077:1097	In addition to the imitation and construction of the natural structure, WF-IVD also achieves favorable mechanical matching and good biocompatibility and possesses unique mechanical buckling buffer characteristics owing to the aligned fiber bundles.
33755430	1	5	theme	due	284:286	arg1	support					247:253	distinctive anisotropic mechanical support	212:253	distinctive anisotropic mechanical support	212:253	Natural intervertebral disks (IVDs) exhibit distinctive anisotropic mechanical support and dissipation performances due to their well-developed special microstructures.
33755430	0	6	theme	Anisotropic	73:83	arg1	Materials					109:117	Highly Anisotropic Wood Hydrogel Composite Materials	66:117	Highly Anisotropic Wood Hydrogel Composite Materials with Mechanical Matching and Buckling Buffering	66:165	Overall Structure Construction of an Intervertebral Disk Based on Highly Anisotropic Wood Hydrogel Composite Materials with Mechanical Matching and Buckling Buffering.
33755430	5	7	theme	fiber	1184:1188	arg1	bundles					1190:1196	the aligned fiber bundles	1172:1196	the aligned fiber bundles	1172:1196	In addition to the imitation and construction of the natural structure, WF-IVD also achieves favorable mechanical matching and good biocompatibility and possesses unique mechanical buckling buffer characteristics owing to the aligned fiber bundles.
33755430	4	8	theme	anisotropic	885:895	arg1	AF					945:946	AF	945:946	AF	945:946	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	8	theme	anisotropic	885:895	arg1	fibrosus					935:942	anisotropic wood cellulose hydrogel-based annulus fibrosus	885:942	anisotropic wood cellulose hydrogel-based annulus fibrosus (AF)	885:947	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	1	9	theme	intervertebral	176:189	arg1	IVDs					198:201	IVDs	198:201	IVDs	198:201	Natural intervertebral disks (IVDs) exhibit distinctive anisotropic mechanical support and dissipation performances due to their well-developed special microstructures.
33755430	1	9	theme	intervertebral	176:189	arg1	disks					191:195	Natural intervertebral disks	168:195	Natural intervertebral disks (IVDs)	168:202	Natural intervertebral disks (IVDs) exhibit distinctive anisotropic mechanical support and dissipation performances due to their well-developed special microstructures.
33755430	0	10	theme	Composite	99:107	arg1	Materials					109:117	Highly Anisotropic Wood Hydrogel Composite Materials	66:117	Highly Anisotropic Wood Hydrogel Composite Materials with Mechanical Matching and Buckling Buffering	66:165	Overall Structure Construction of an Intervertebral Disk Based on Highly Anisotropic Wood Hydrogel Composite Materials with Mechanical Matching and Buckling Buffering.
33755430	4	11	theme	hydrogel-based	912:925	arg1	AF					945:946	AF	945:946	AF	945:946	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	11	theme	hydrogel-based	912:925	arg1	fibrosus					935:942	anisotropic wood cellulose hydrogel-based annulus fibrosus	885:942	anisotropic wood cellulose hydrogel-based annulus fibrosus (AF)	885:947	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	12	theme	wood	767:770	arg1	WF-IVD					787:792	WF-IVD	787:792	WF-IVD	787:792	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	12	theme	wood	767:770	arg1	IVD					782:784	a complete wood framework IVD	756:784	a complete wood framework IVD (WF-IVD)	756:793	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	0	13	theme	Hydrogel	90:97	arg1	Materials					109:117	Highly Anisotropic Wood Hydrogel Composite Materials	66:117	Highly Anisotropic Wood Hydrogel Composite Materials with Mechanical Matching and Buckling Buffering	66:165	Overall Structure Construction of an Intervertebral Disk Based on Highly Anisotropic Wood Hydrogel Composite Materials with Mechanical Matching and Buckling Buffering.
33755430	5	14	theme	buffer	1140:1145	arg1	characteristics					1147:1161	unique mechanical buckling buffer characteristics	1113:1161	unique mechanical buckling buffer characteristics owing to the aligned fiber bundles	1113:1196	In addition to the imitation and construction of the natural structure, WF-IVD also achieves favorable mechanical matching and good biocompatibility and possesses unique mechanical buckling buffer characteristics owing to the aligned fiber bundles.
33755430	4	15	theme	cellulose	902:910	arg1	AF					945:946	AF	945:946	AF	945:946	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	15	theme	cellulose	902:910	arg1	fibrosus					935:942	anisotropic wood cellulose hydrogel-based annulus fibrosus	885:942	anisotropic wood cellulose hydrogel-based annulus fibrosus (AF)	885:947	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	2	16	theme	function	390:397	arg1	absence					379:385	the absence	375:385	the absence of function	375:397	As the intact IVD structure degrades, the absence of function will lead to severe backache.
33755430	1	17	theme	well-developed	297:310	arg1	microstructures					320:334	their well-developed special microstructures	291:334	their well-developed special microstructures	291:334	Natural intervertebral disks (IVDs) exhibit distinctive anisotropic mechanical support and dissipation performances due to their well-developed special microstructures.
33755430	4	18	theme	overall	578:584	arg1	construction					586:597	overall construction	578:597	overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel)	578:753	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	6	19	dep	mimicking	1246:1254	arg1	the					1242:1244	the	1242:1244	the	1242:1244	This study offers a promising strategy for the mimicking and construction of complex native tissues.
33755430	6	20	theme	promising	1219:1227	arg1	strategy					1229:1236	a promising strategy	1217:1236	a promising strategy for the mimicking and construction of complex native tissues	1217:1297	This study offers a promising strategy for the mimicking and construction of complex native tissues.
33755430	4	21	theme	in	701:702	arg1	IVD					636:638	native IVD	629:638	native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel)	629:753	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	21	theme	in	701:702	arg1	polymerization					709:722	in situ polymerization	701:722	in situ polymerization of the nanocomposite hydrogel	701:752	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	22	theme	framework	687:695	arg1	IVD					636:638	native IVD	629:638	native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel)	629:753	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	22	theme	framework	687:695	arg1	polymerization					709:722	in situ polymerization	701:722	in situ polymerization of the nanocomposite hydrogel	701:752	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	22	theme	framework	687:695	arg1	extraction					641:650	extraction	641:650	extraction of the naturally aligned cellulose framework	641:695	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	23	theme	structure	616:624	arg1	construction					586:597	overall construction	578:597	overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel)	578:753	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	24	theme	two-phase	606:614	arg1	structure					616:624	the two-phase structure	602:624	the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel)	602:753	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	5	25	theme	natural	1003:1009	arg1	structure					1011:1019	the natural structure	999:1019	the natural structure	999:1019	In addition to the imitation and construction of the natural structure, WF-IVD also achieves favorable mechanical matching and good biocompatibility and possesses unique mechanical buckling buffer characteristics owing to the aligned fiber bundles.
33755430	3	26	theme	characteristic	470:483	arg1	structure					485:493	characteristic structure	470:493	characteristic structure	470:493	However, the complete simulation for the characteristic structure and function of native IVD is unattainable using current methods.
33755430	0	27	theme	Structure	8:16	arg1	Construction					18:29	Overall Structure Construction	0:29	Overall Structure Construction of an Intervertebral Disk Based on Highly Anisotropic Wood Hydrogel Composite Materials with Mechanical Matching and Buckling Buffering.	0:166	Overall Structure Construction of an Intervertebral Disk Based on Highly Anisotropic Wood Hydrogel Composite Materials with Mechanical Matching and Buckling Buffering.
33755430	0	28	with	Materials	109:117	arg1	Matching					135:142	Matching	135:142	Matching	135:142	Overall Structure Construction of an Intervertebral Disk Based on Highly Anisotropic Wood Hydrogel Composite Materials with Mechanical Matching and Buckling Buffering.
33755430	0	28	with	Materials	109:117	arg1	Buffering					157:165	Buckling Buffering	148:165	Buckling Buffering	148:165	Overall Structure Construction of an Intervertebral Disk Based on Highly Anisotropic Wood Hydrogel Composite Materials with Mechanical Matching and Buckling Buffering.
33755430	4	29	theme	complete	758:765	arg1	WF-IVD					787:792	WF-IVD	787:792	WF-IVD	787:792	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	29	theme	complete	758:765	arg1	IVD					782:784	a complete wood framework IVD	756:784	a complete wood framework IVD (WF-IVD)	756:793	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	2	30	theme	severe	412:417	arg1	backache					419:426	severe backache	412:426	severe backache	412:426	As the intact IVD structure degrades, the absence of function will lead to severe backache.
33755430	0	31	theme	Overall	0:6	arg1	Construction					18:29	Overall Structure Construction	0:29	Overall Structure Construction of an Intervertebral Disk Based on Highly Anisotropic Wood Hydrogel Composite Materials with Mechanical Matching and Buckling Buffering.	0:166	Overall Structure Construction of an Intervertebral Disk Based on Highly Anisotropic Wood Hydrogel Composite Materials with Mechanical Matching and Buckling Buffering.
33755430	1	32	theme	special	312:318	arg1	microstructures					320:334	their well-developed special microstructures	291:334	their well-developed special microstructures	291:334	Natural intervertebral disks (IVDs) exhibit distinctive anisotropic mechanical support and dissipation performances due to their well-developed special microstructures.
33755430	2	33	theme	IVD	351:353	arg1	structure					355:363	the intact IVD structure	340:363	the intact IVD structure	340:363	As the intact IVD structure degrades, the absence of function will lead to severe backache.
33755430	5	34	theme	unique	1113:1118	arg1	characteristics					1147:1161	unique mechanical buckling buffer characteristics	1113:1161	unique mechanical buckling buffer characteristics owing to the aligned fiber bundles	1113:1196	In addition to the imitation and construction of the natural structure, WF-IVD also achieves favorable mechanical matching and good biocompatibility and possesses unique mechanical buckling buffer characteristics owing to the aligned fiber bundles.
33755430	2	35	theme	intact	344:349	arg1	structure					355:363	the intact IVD structure	340:363	the intact IVD structure	340:363	As the intact IVD structure degrades, the absence of function will lead to severe backache.
33755430	5	36	contain	possesses	1103:1111	arg1	WF-IVD					1022:1027	WF-IVD	1022:1027	WF-IVD	1022:1027	In addition to the imitation and construction of the natural structure, WF-IVD also achieves favorable mechanical matching and good biocompatibility and possesses unique mechanical buckling buffer characteristics owing to the aligned fiber bundles.
33755430	5	36	contain	possesses	1103:1111	arg2	characteristics					1147:1161	unique mechanical buckling buffer characteristics	1113:1161	unique mechanical buckling buffer characteristics owing to the aligned fiber bundles	1113:1196	In addition to the imitation and construction of the natural structure, WF-IVD also achieves favorable mechanical matching and good biocompatibility and possesses unique mechanical buckling buffer characteristics owing to the aligned fiber bundles.
33755430	5	37	theme	aligned	1176:1182	arg1	bundles					1190:1196	the aligned fiber bundles	1172:1196	the aligned fiber bundles	1172:1196	In addition to the imitation and construction of the natural structure, WF-IVD also achieves favorable mechanical matching and good biocompatibility and possesses unique mechanical buckling buffer characteristics owing to the aligned fiber bundles.
33755430	3	38	theme	current	544:550	arg1	methods					552:558	current methods	544:558	current methods	544:558	However, the complete simulation for the characteristic structure and function of native IVD is unattainable using current methods.
33755430	1	39	theme	distinctive	212:222	arg1	support					247:253	distinctive anisotropic mechanical support	212:253	distinctive anisotropic mechanical support	212:253	Natural intervertebral disks (IVDs) exhibit distinctive anisotropic mechanical support and dissipation performances due to their well-developed special microstructures.
33755430	0	40	theme	Intervertebral	37:50	arg1	Based					57:61	an Intervertebral Disk Based	34:61	an Intervertebral Disk Based	34:61	Overall Structure Construction of an Intervertebral Disk Based on Highly Anisotropic Wood Hydrogel Composite Materials with Mechanical Matching and Buckling Buffering.
33755430	0	41	theme	Buckling	148:155	arg1	Buffering					157:165	Buckling Buffering	148:165	Buckling Buffering	148:165	Overall Structure Construction of an Intervertebral Disk Based on Highly Anisotropic Wood Hydrogel Composite Materials with Mechanical Matching and Buckling Buffering.
33755430	4	42	theme	aligned	669:675	arg1	framework					687:695	the naturally aligned cellulose framework	655:695	the naturally aligned cellulose framework	655:695	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	43	theme	hydrogel-based	844:857	arg1	NP					877:878	NP	877:878	NP	877:878	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	43	theme	hydrogel-based	844:857	arg1	pulposus					867:874	elastic nanocomposite hydrogel-based nucleus pulposus	822:874	elastic nanocomposite hydrogel-based nucleus pulposus (NP)	822:879	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	1	44	theme	anisotropic	224:234	arg1	support					247:253	distinctive anisotropic mechanical support	212:253	distinctive anisotropic mechanical support	212:253	Natural intervertebral disks (IVDs) exhibit distinctive anisotropic mechanical support and dissipation performances due to their well-developed special microstructures.
33755430	3	45	theme	complete	442:449	arg1	simulation					451:460	the complete simulation	438:460	the complete simulation for the characteristic structure and function of native IVD	438:520	However, the complete simulation for the characteristic structure and function of native IVD is unattainable using current methods.
33755430	3	45	theme	complete	442:449	arg1	unattainable					525:536	unattainable	525:536	unattainable	525:536	However, the complete simulation for the characteristic structure and function of native IVD is unattainable using current methods.
33755430	5	46	theme	mechanical	1120:1129	arg1	characteristics					1147:1161	unique mechanical buckling buffer characteristics	1113:1161	unique mechanical buckling buffer characteristics owing to the aligned fiber bundles	1113:1196	In addition to the imitation and construction of the natural structure, WF-IVD also achieves favorable mechanical matching and good biocompatibility and possesses unique mechanical buckling buffer characteristics owing to the aligned fiber bundles.
33755430	4	47	theme	nanocomposite	830:842	arg1	NP					877:878	NP	877:878	NP	877:878	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	47	theme	nanocomposite	830:842	arg1	pulposus					867:874	elastic nanocomposite hydrogel-based nucleus pulposus	822:874	elastic nanocomposite hydrogel-based nucleus pulposus (NP)	822:879	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	1	48	theme	mechanical	236:245	arg1	support					247:253	distinctive anisotropic mechanical support	212:253	distinctive anisotropic mechanical support	212:253	Natural intervertebral disks (IVDs) exhibit distinctive anisotropic mechanical support and dissipation performances due to their well-developed special microstructures.
33755430	0	49	theme	Based	57:61	arg1	Construction					18:29	Overall Structure Construction	0:29	Overall Structure Construction of an Intervertebral Disk Based on Highly Anisotropic Wood Hydrogel Composite Materials with Mechanical Matching and Buckling Buffering.	0:166	Overall Structure Construction of an Intervertebral Disk Based on Highly Anisotropic Wood Hydrogel Composite Materials with Mechanical Matching and Buckling Buffering.
33755430	5	50	theme	buckling	1131:1138	arg1	characteristics					1147:1161	unique mechanical buckling buffer characteristics	1113:1161	unique mechanical buckling buffer characteristics owing to the aligned fiber bundles	1113:1196	In addition to the imitation and construction of the natural structure, WF-IVD also achieves favorable mechanical matching and good biocompatibility and possesses unique mechanical buckling buffer characteristics owing to the aligned fiber bundles.
33755430	6	51	theme	complex	1276:1282	arg1	tissues					1291:1297	complex native tissues	1276:1297	complex native tissues	1276:1297	This study offers a promising strategy for the mimicking and construction of complex native tissues.
33755430	4	52	theme	hydrogel	745:752	arg1	IVD					636:638	native IVD	629:638	native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel)	629:753	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	52	theme	hydrogel	745:752	arg1	polymerization					709:722	in situ polymerization	701:722	in situ polymerization of the nanocomposite hydrogel	701:752	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	52	theme	hydrogel	745:752	arg1	extraction					641:650	extraction	641:650	extraction of the naturally aligned cellulose framework	641:695	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	0	53	theme	Disk	52:55	arg1	Based					57:61	an Intervertebral Disk Based	34:61	an Intervertebral Disk Based	34:61	Overall Structure Construction of an Intervertebral Disk Based on Highly Anisotropic Wood Hydrogel Composite Materials with Mechanical Matching and Buckling Buffering.
33755430	4	54	theme	annulus	927:933	arg1	AF					945:946	AF	945:946	AF	945:946	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	54	theme	annulus	927:933	arg1	fibrosus					935:942	anisotropic wood cellulose hydrogel-based annulus fibrosus	885:942	anisotropic wood cellulose hydrogel-based annulus fibrosus (AF)	885:947	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	55	theme	nucleus	859:865	arg1	NP					877:878	NP	877:878	NP	877:878	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	55	theme	nucleus	859:865	arg1	pulposus					867:874	elastic nanocomposite hydrogel-based nucleus pulposus	822:874	elastic nanocomposite hydrogel-based nucleus pulposus (NP)	822:879	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	56	theme	nanocomposite	731:743	arg1	hydrogel					745:752	the nanocomposite hydrogel	727:752	the nanocomposite hydrogel	727:752	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	57	dep	in	701:702	arg1	situ					704:707	situ	704:707	situ	704:707	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	3	58	dep	structure	485:493	arg1	the					466:468	the	466:468	the	466:468	However, the complete simulation for the characteristic structure and function of native IVD is unattainable using current methods.
33755430	6	59	theme	native	1284:1289	arg1	tissues					1291:1297	complex native tissues	1276:1297	complex native tissues	1276:1297	This study offers a promising strategy for the mimicking and construction of complex native tissues.
33755430	3	60	theme	native	511:516	arg1	IVD					518:520	native IVD	511:520	native IVD	511:520	However, the complete simulation for the characteristic structure and function of native IVD is unattainable using current methods.
33755430	4	61	theme	framework	772:780	arg1	WF-IVD					787:792	WF-IVD	787:792	WF-IVD	787:792	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	61	theme	framework	772:780	arg1	IVD					782:784	a complete wood framework IVD	756:784	a complete wood framework IVD (WF-IVD)	756:793	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	62	theme	IVD	636:638	arg1	structure					616:624	the two-phase structure	602:624	the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel)	602:753	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	63	dep	IVD	636:638	arg1	IVD					636:638	native IVD	629:638	native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel)	629:753	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	63	dep	IVD	636:638	arg1	polymerization					709:722	in situ polymerization	701:722	in situ polymerization of the nanocomposite hydrogel	701:752	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	63	dep	IVD	636:638	arg1	extraction					641:650	extraction	641:650	extraction of the naturally aligned cellulose framework	641:695	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	3	64	theme	IVD	518:520	arg1	function					499:506	function	499:506	function	499:506	However, the complete simulation for the characteristic structure and function of native IVD is unattainable using current methods.
33755430	3	64	theme	IVD	518:520	arg1	structure					485:493	characteristic structure	470:493	characteristic structure	470:493	However, the complete simulation for the characteristic structure and function of native IVD is unattainable using current methods.
33755430	4	65	theme	native	629:634	arg1	IVD					636:638	native IVD	629:638	native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel)	629:753	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	65	theme	native	629:634	arg1	polymerization					709:722	in situ polymerization	701:722	in situ polymerization of the nanocomposite hydrogel	701:752	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	65	theme	native	629:634	arg1	extraction					641:650	extraction	641:650	extraction of the naturally aligned cellulose framework	641:695	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	66	theme	elastic	822:828	arg1	NP					877:878	NP	877:878	NP	877:878	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	4	66	theme	elastic	822:828	arg1	pulposus					867:874	elastic nanocomposite hydrogel-based nucleus pulposus	822:874	elastic nanocomposite hydrogel-based nucleus pulposus (NP)	822:879	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	5	67	dep	imitation	969:977	arg1	the					965:967	the	965:967	the	965:967	In addition to the imitation and construction of the natural structure, WF-IVD also achieves favorable mechanical matching and good biocompatibility and possesses unique mechanical buckling buffer characteristics owing to the aligned fiber bundles.
33755430	5	67	dep	imitation	969:977	arg1	addition					953:960	addition	953:960	addition	953:960	In addition to the imitation and construction of the natural structure, WF-IVD also achieves favorable mechanical matching and good biocompatibility and possesses unique mechanical buckling buffer characteristics owing to the aligned fiber bundles.
33755430	4	68	theme	cellulose	677:685	arg1	framework					687:695	the naturally aligned cellulose framework	655:695	the naturally aligned cellulose framework	655:695	In this work, by overall construction of the two-phase structure of native IVD (extraction of the naturally aligned cellulose framework and in situ polymerization of the nanocomposite hydrogel), a complete wood framework IVD (WF-IVD) is manufactured containing elastic nanocomposite hydrogel-based nucleus pulposus (NP) and anisotropic wood cellulose hydrogel-based annulus fibrosus (AF).
33755430	1	69	theme	dissipation	259:269	arg1	performances					271:282	dissipation performances	259:282	dissipation performances	259:282	Natural intervertebral disks (IVDs) exhibit distinctive anisotropic mechanical support and dissipation performances due to their well-developed special microstructures.
33755430	5	70	theme	structure	1011:1019	arg1	imitation					969:977	imitation	969:977	imitation	969:977	In addition to the imitation and construction of the natural structure, WF-IVD also achieves favorable mechanical matching and good biocompatibility and possesses unique mechanical buckling buffer characteristics owing to the aligned fiber bundles.
33755430	5	70	theme	structure	1011:1019	arg1	construction					983:994	construction	983:994	construction	983:994	In addition to the imitation and construction of the natural structure, WF-IVD also achieves favorable mechanical matching and good biocompatibility and possesses unique mechanical buckling buffer characteristics owing to the aligned fiber bundles.
34147122	12	0	theme	plant	1914:1918	arg1	polysaccharides					1920:1934	non-toxic plant polysaccharides	1904:1934	non-toxic plant polysaccharides such as β-mannan	1904:1951	Furthermore, cells modified with non-toxic plant polysaccharides such as β-mannan offer a modular chassis to produce and encapsulate sensitive cargo such as therapeutic proteins.
34147122	12	0	theme	plant	1914:1918	arg1	β-mannan					1944:1951	β-mannan	1944:1951	β-mannan	1944:1951	Furthermore, cells modified with non-toxic plant polysaccharides such as β-mannan offer a modular chassis to produce and encapsulate sensitive cargo such as therapeutic proteins.
34147122	4	1	theme	optimized	623:631	arg1	platform					649:656	an optimized Pichia pastoris platform	620:656	an optimized Pichia pastoris platform to produce tunable heteromannan (HM) polysaccharides in yeast	620:718	RESULTS Here, we assembled cellulose synthase-like A (CSLA) enzymes using an optimized Pichia pastoris platform to produce tunable heteromannan (HM) polysaccharides in yeast.
34147122	7	2	theme	CSLA	1192:1195	arg1	mannan					1202:1207	CSLA pure mannan synthases	1192:1217	CSLA pure mannan synthases	1192:1217	However, an engineered glucomannan synthase as well as CSLA pure mannan synthases and a CSLC glucan synthase did not inhibit growth.
34147122	9	3	theme	CSLA	1481:1484	arg1	proteins					1486:1493	chimeric CSLA proteins	1472:1493	chimeric CSLA proteins	1472:1493	HM yield and glucose incorporation could be further increased by co-expressing chimeric CSLA proteins with a MANNAN-SYNTHESIS-RELATED (MSR) co-factor from Arabidopsis thaliana.
34147122	6	4	theme	Prolonged	953:961	arg1	expression					963:972	Prolonged expression	953:972	Prolonged expression of a glucomannan synthase from Amorphophallus konjac	953:1025	Prolonged expression of a glucomannan synthase from Amorphophallus konjac was toxic to yeast cells: reducing biomass accumulation and ultimately leading to compromised cell viability.
34147122	4	5	theme	pastoris	640:647	arg1	platform					649:656	an optimized Pichia pastoris platform	620:656	an optimized Pichia pastoris platform to produce tunable heteromannan (HM) polysaccharides in yeast	620:718	RESULTS Here, we assembled cellulose synthase-like A (CSLA) enzymes using an optimized Pichia pastoris platform to produce tunable heteromannan (HM) polysaccharides in yeast.
34147122	10	6	theme	polysaccharide-based	1637:1656	arg1	biomaterials					1658:1669	polysaccharide-based biomaterials	1637:1669	polysaccharide-based biomaterials that are needed for a sustainable bioeconomy	1637:1714	CONCLUSION The results provide novel routes for the engineering of polysaccharide-based biomaterials that are needed for a sustainable bioeconomy.
34147122	11	7	theme	enzymes	1774:1780	arg1	characterization					1721:1736	The characterization	1717:1736	The characterization of chimeric cellulose synthase-like enzymes in yeast	1717:1789	The characterization of chimeric cellulose synthase-like enzymes in yeast offers an exciting avenue to produce plant polysaccharides in a tunable manner.
34147122	9	8	theme	MANNAN-SYNTHESIS-RELATED	1502:1525	arg1	co-factor					1533:1541	a MANNAN-SYNTHESIS-RELATED (MSR) co-factor	1500:1541	a MANNAN-SYNTHESIS-RELATED (MSR) co-factor from Arabidopsis thaliana	1500:1567	HM yield and glucose incorporation could be further increased by co-expressing chimeric CSLA proteins with a MANNAN-SYNTHESIS-RELATED (MSR) co-factor from Arabidopsis thaliana.
34147122	7	9	theme	engineered	1149:1158	arg1	synthase					1172:1179	an engineered glucomannan synthase	1146:1179	an engineered glucomannan synthase as well as CSLA pure mannan synthases and a CSLC glucan synthase	1146:1244	However, an engineered glucomannan synthase as well as CSLA pure mannan synthases and a CSLC glucan synthase did not inhibit growth.
34147122	9	10	theme	MSR	1528:1530	arg1	co-factor					1533:1541	a MANNAN-SYNTHESIS-RELATED (MSR) co-factor	1500:1541	a MANNAN-SYNTHESIS-RELATED (MSR) co-factor from Arabidopsis thaliana	1500:1567	HM yield and glucose incorporation could be further increased by co-expressing chimeric CSLA proteins with a MANNAN-SYNTHESIS-RELATED (MSR) co-factor from Arabidopsis thaliana.
34147122	5	11	theme	plant	748:752	arg1	mannan					754:759	plant mannan and glucomannan synthases	748:785	mannan	754:759	By swapping the domains of plant mannan and glucomannan synthases, we engineered chimeric CSLA proteins that made β-1,4-linked mannan in quantities surpassing those of the native enzymes while minimizing the burden on yeast growth.
34147122	2	12	theme	polysaccharide-based	298:317	arg1	biomaterials					319:330	polysaccharide-based biomaterials	298:330	polysaccharide-based biomaterials	298:330	In the past 5 years, exciting possibilities have emerged in the engineering of polysaccharide-based biomaterials.
34147122	9	13	theme	Arabidopsis	1548:1558	arg1	thaliana					1560:1567	Arabidopsis thaliana	1548:1567	Arabidopsis thaliana	1548:1567	HM yield and glucose incorporation could be further increased by co-expressing chimeric CSLA proteins with a MANNAN-SYNTHESIS-RELATED (MSR) co-factor from Arabidopsis thaliana.
34147122	8	14	theme	Pichia	1285:1290	arg1	size					1297:1300	Pichia cell size	1285:1300	Pichia cell size	1285:1300	Interestingly, Pichia cell size could be increased or decreased depending on the composition of the CSLA protein sequence.
34147122	6	15	theme	glucomannan	979:989	arg1	synthase					991:998	a glucomannan synthase	977:998	a glucomannan synthase from Amorphophallus konjac	977:1025	Prolonged expression of a glucomannan synthase from Amorphophallus konjac was toxic to yeast cells: reducing biomass accumulation and ultimately leading to compromised cell viability.
34147122	8	16	theme	CSLA	1370:1373	arg1	sequence					1383:1390	the CSLA protein sequence	1366:1390	the CSLA protein sequence	1366:1390	Interestingly, Pichia cell size could be increased or decreased depending on the composition of the CSLA protein sequence.
34147122	5	17	theme	chimeric	802:809	arg1	proteins					816:823	chimeric CSLA proteins	802:823	chimeric CSLA proteins that made β-1,4-linked mannan in quantities surpassing those of the native enzymes while minimizing the burden on yeast growth	802:950	By swapping the domains of plant mannan and glucomannan synthases, we engineered chimeric CSLA proteins that made β-1,4-linked mannan in quantities surpassing those of the native enzymes while minimizing the burden on yeast growth.
34147122	2	18	theme	exciting	240:247	arg1	possibilities					249:261	exciting possibilities	240:261	exciting possibilities	240:261	In the past 5 years, exciting possibilities have emerged in the engineering of polysaccharide-based biomaterials.
34147122	5	19	theme	yeast	939:943	arg1	growth					945:950	yeast growth	939:950	yeast growth	939:950	By swapping the domains of plant mannan and glucomannan synthases, we engineered chimeric CSLA proteins that made β-1,4-linked mannan in quantities surpassing those of the native enzymes while minimizing the burden on yeast growth.
34147122	6	20	theme	biomass	1062:1068	arg1	accumulation					1070:1081	biomass accumulation	1062:1081	biomass accumulation	1062:1081	Prolonged expression of a glucomannan synthase from Amorphophallus konjac was toxic to yeast cells: reducing biomass accumulation and ultimately leading to compromised cell viability.
34147122	3	21	theme	impressive	341:350	arg1	advances					352:359	impressive advances	341:359	impressive advances on bacterial cellulose-based hydrogels	341:398	Despite impressive advances on bacterial cellulose-based hydrogels, comparatively little is known about how plant hemicelluloses can be reconstituted and modulated in cells suitable for biotechnological purposes.
34147122	1	22	dep	BACKGROUND	75:84	arg1	have					182:185	have	182:185	have valuable biotechnological uses	182:216	BACKGROUND The carbohydrate polymers that encapsulate plants cells have benefited humans for centuries and have valuable biotechnological uses.
34147122	1	22	dep	BACKGROUND	75:84	arg1	have					142:145	have	142:145	have benefited humans for centuries	142:176	BACKGROUND The carbohydrate polymers that encapsulate plants cells have benefited humans for centuries and have valuable biotechnological uses.
34147122	5	23	theme	synthases	777:785	arg1	domains					737:743	the domains	733:743	the domains of plant mannan and glucomannan synthases	733:785	By swapping the domains of plant mannan and glucomannan synthases, we engineered chimeric CSLA proteins that made β-1,4-linked mannan in quantities surpassing those of the native enzymes while minimizing the burden on yeast growth.
34147122	5	23	theme	synthases	777:785	arg1	mannan					754:759	plant mannan and glucomannan synthases	748:785	mannan	754:759	By swapping the domains of plant mannan and glucomannan synthases, we engineered chimeric CSLA proteins that made β-1,4-linked mannan in quantities surpassing those of the native enzymes while minimizing the burden on yeast growth.
34147122	5	23	theme	synthases	777:785	arg1	synthases					777:785	plant mannan and glucomannan synthases	748:785	synthases	777:785	By swapping the domains of plant mannan and glucomannan synthases, we engineered chimeric CSLA proteins that made β-1,4-linked mannan in quantities surpassing those of the native enzymes while minimizing the burden on yeast growth.
34147122	6	24	theme	yeast	1040:1044	arg1	cells					1046:1050	yeast cells	1040:1050	yeast cells: reducing biomass accumulation and ultimately leading to compromised cell viability	1040:1134	Prolonged expression of a glucomannan synthase from Amorphophallus konjac was toxic to yeast cells: reducing biomass accumulation and ultimately leading to compromised cell viability.
34147122	3	25	theme	cellulose-based	374:388	arg1	hydrogels					390:398	bacterial cellulose-based hydrogels	364:398	bacterial cellulose-based hydrogels	364:398	Despite impressive advances on bacterial cellulose-based hydrogels, comparatively little is known about how plant hemicelluloses can be reconstituted and modulated in cells suitable for biotechnological purposes.
34147122	10	26	dep	CONCLUSION	1570:1579	arg1	provide					1593:1599	provide	1593:1599	provide novel routes for the engineering of polysaccharide-based biomaterials that are needed for a sustainable bioeconomy	1593:1714	CONCLUSION The results provide novel routes for the engineering of polysaccharide-based biomaterials that are needed for a sustainable bioeconomy.
34147122	11	27	from	enzymes	1774:1780	arg1	yeast					1785:1789	yeast	1785:1789	yeast	1785:1789	The characterization of chimeric cellulose synthase-like enzymes in yeast offers an exciting avenue to produce plant polysaccharides in a tunable manner.
34147122	0	28	theme	Modular	0:6	arg1	biosynthesis					8:19	Modular biosynthesis	0:19	Modular biosynthesis of plant hemicellulose	0:42	Modular biosynthesis of plant hemicellulose and its impact on yeast cells.
34147122	4	29	dep	RESULTS	546:552	arg1	assembled					563:571	assembled	563:571	assembled cellulose synthase-like A (CSLA) enzymes using an optimized Pichia pastoris platform to produce tunable heteromannan (HM) polysaccharides in yeast	563:718	RESULTS Here, we assembled cellulose synthase-like A (CSLA) enzymes using an optimized Pichia pastoris platform to produce tunable heteromannan (HM) polysaccharides in yeast.
34147122	11	30	from	yeast	1785:1789	arg1	characterization					1721:1736	The characterization	1717:1736	The characterization of chimeric cellulose synthase-like enzymes in yeast	1717:1789	The characterization of chimeric cellulose synthase-like enzymes in yeast offers an exciting avenue to produce plant polysaccharides in a tunable manner.
34147122	3	31	theme	suitable	506:513	arg1	cells					500:504	cells	500:504	cells suitable for biotechnological purposes	500:543	Despite impressive advances on bacterial cellulose-based hydrogels, comparatively little is known about how plant hemicelluloses can be reconstituted and modulated in cells suitable for biotechnological purposes.
34147122	7	32	theme	CSLC	1225:1228	arg1	glucan					1230:1235	a CSLC glucan	1223:1235	a CSLC glucan	1223:1235	However, an engineered glucomannan synthase as well as CSLA pure mannan synthases and a CSLC glucan synthase did not inhibit growth.
34147122	6	33	theme	compromised	1109:1119	arg1	viability					1126:1134	compromised cell viability	1109:1134	compromised cell viability	1109:1134	Prolonged expression of a glucomannan synthase from Amorphophallus konjac was toxic to yeast cells: reducing biomass accumulation and ultimately leading to compromised cell viability.
34147122	1	34	contain	have	182:185	arg2	biotechnological					196:211	biotechnological	196:211	biotechnological	196:211	BACKGROUND The carbohydrate polymers that encapsulate plants cells have benefited humans for centuries and have valuable biotechnological uses.
34147122	1	34	contain	have	182:185	arg1	polymers					103:110	The carbohydrate polymers	86:110	The carbohydrate polymers that encapsulate plants cells	86:140	BACKGROUND The carbohydrate polymers that encapsulate plants cells have benefited humans for centuries and have valuable biotechnological uses.
34147122	0	35	theme	hemicellulose	30:42	arg1	biosynthesis					8:19	Modular biosynthesis	0:19	Modular biosynthesis of plant hemicellulose	0:42	Modular biosynthesis of plant hemicellulose and its impact on yeast cells.
34147122	0	35	theme	hemicellulose	30:42	arg1	impact					52:57	its impact	48:57	its impact on yeast cells	48:72	Modular biosynthesis of plant hemicellulose and its impact on yeast cells.
34147122	9	36	theme	glucose	1406:1412	arg1	incorporation					1414:1426	glucose incorporation	1406:1426	glucose incorporation	1406:1426	HM yield and glucose incorporation could be further increased by co-expressing chimeric CSLA proteins with a MANNAN-SYNTHESIS-RELATED (MSR) co-factor from Arabidopsis thaliana.
34147122	4	37	theme	cellulose	573:581	arg1	A					597:597	cellulose synthase-like A	573:597	cellulose synthase-like A (CSLA) enzymes	573:612	RESULTS Here, we assembled cellulose synthase-like A (CSLA) enzymes using an optimized Pichia pastoris platform to produce tunable heteromannan (HM) polysaccharides in yeast.
34147122	4	37	theme	cellulose	573:581	arg1	CSLA					600:603	CSLA	600:603	CSLA	600:603	RESULTS Here, we assembled cellulose synthase-like A (CSLA) enzymes using an optimized Pichia pastoris platform to produce tunable heteromannan (HM) polysaccharides in yeast.
34147122	11	38	theme	exciting	1801:1808	arg1	avenue					1810:1815	an exciting avenue	1798:1815	an exciting avenue to produce plant polysaccharides in a tunable manner	1798:1868	The characterization of chimeric cellulose synthase-like enzymes in yeast offers an exciting avenue to produce plant polysaccharides in a tunable manner.
34147122	7	39	theme	mannan	1202:1207	arg1	synthase					1237:1244	CSLA pure mannan synthases and a CSLC glucan synthase	1192:1244	an engineered glucomannan synthase as well as CSLA pure mannan synthases and a CSLC glucan synthase	1146:1244	However, an engineered glucomannan synthase as well as CSLA pure mannan synthases and a CSLC glucan synthase did not inhibit growth.
34147122	4	40	theme	A	597:597	arg1	enzymes					606:612	cellulose synthase-like A (CSLA) enzymes	573:612	cellulose synthase-like A (CSLA) enzymes	573:612	RESULTS Here, we assembled cellulose synthase-like A (CSLA) enzymes using an optimized Pichia pastoris platform to produce tunable heteromannan (HM) polysaccharides in yeast.
34147122	3	41	theme	biotechnological	519:534	arg1	purposes					536:543	biotechnological purposes	519:543	biotechnological purposes	519:543	Despite impressive advances on bacterial cellulose-based hydrogels, comparatively little is known about how plant hemicelluloses can be reconstituted and modulated in cells suitable for biotechnological purposes.
34147122	12	42	theme	non-toxic	1904:1912	arg1	polysaccharides					1920:1934	non-toxic plant polysaccharides	1904:1934	non-toxic plant polysaccharides such as β-mannan	1904:1951	Furthermore, cells modified with non-toxic plant polysaccharides such as β-mannan offer a modular chassis to produce and encapsulate sensitive cargo such as therapeutic proteins.
34147122	12	42	theme	non-toxic	1904:1912	arg1	β-mannan					1944:1951	β-mannan	1944:1951	β-mannan	1944:1951	Furthermore, cells modified with non-toxic plant polysaccharides such as β-mannan offer a modular chassis to produce and encapsulate sensitive cargo such as therapeutic proteins.
34147122	1	43	theme	benefited	147:155	arg1	humans					157:162	benefited humans	147:162	benefited humans for centuries	147:176	BACKGROUND The carbohydrate polymers that encapsulate plants cells have benefited humans for centuries and have valuable biotechnological uses.
34147122	6	44	from	expression	963:972	arg1	konjac					1020:1025	Amorphophallus konjac	1005:1025	Amorphophallus konjac	1005:1025	Prolonged expression of a glucomannan synthase from Amorphophallus konjac was toxic to yeast cells: reducing biomass accumulation and ultimately leading to compromised cell viability.
34147122	11	45	theme	plant	1828:1832	arg1	polysaccharides					1834:1848	plant polysaccharides	1828:1848	plant polysaccharides	1828:1848	The characterization of chimeric cellulose synthase-like enzymes in yeast offers an exciting avenue to produce plant polysaccharides in a tunable manner.
34147122	1	46	dep	biotechnological	196:211	arg1	uses					213:216	uses	213:216	uses	213:216	BACKGROUND The carbohydrate polymers that encapsulate plants cells have benefited humans for centuries and have valuable biotechnological uses.
34147122	5	47	theme	β-1,4-linked	835:846	arg1	mannan					848:853	β-1,4-linked mannan	835:853	β-1,4-linked mannan	835:853	By swapping the domains of plant mannan and glucomannan synthases, we engineered chimeric CSLA proteins that made β-1,4-linked mannan in quantities surpassing those of the native enzymes while minimizing the burden on yeast growth.
34147122	11	48	theme	synthase-like	1760:1772	arg1	enzymes					1774:1780	chimeric cellulose synthase-like enzymes	1741:1780	chimeric cellulose synthase-like enzymes in yeast	1741:1789	The characterization of chimeric cellulose synthase-like enzymes in yeast offers an exciting avenue to produce plant polysaccharides in a tunable manner.
34147122	9	49	theme	chimeric	1472:1479	arg1	proteins					1486:1493	chimeric CSLA proteins	1472:1493	chimeric CSLA proteins	1472:1493	HM yield and glucose incorporation could be further increased by co-expressing chimeric CSLA proteins with a MANNAN-SYNTHESIS-RELATED (MSR) co-factor from Arabidopsis thaliana.
34147122	11	50	theme	chimeric	1741:1748	arg1	enzymes					1774:1780	chimeric cellulose synthase-like enzymes	1741:1780	chimeric cellulose synthase-like enzymes in yeast	1741:1789	The characterization of chimeric cellulose synthase-like enzymes in yeast offers an exciting avenue to produce plant polysaccharides in a tunable manner.
34147122	8	51	theme	sequence	1383:1390	arg1	composition					1351:1361	the composition	1347:1361	the composition of the CSLA protein sequence	1347:1390	Interestingly, Pichia cell size could be increased or decreased depending on the composition of the CSLA protein sequence.
34147122	4	52	theme	Pichia	633:638	arg1	pastoris					640:647	Pichia pastoris	633:647	an optimized Pichia pastoris platform to produce tunable heteromannan (HM) polysaccharides in yeast	620:718	RESULTS Here, we assembled cellulose synthase-like A (CSLA) enzymes using an optimized Pichia pastoris platform to produce tunable heteromannan (HM) polysaccharides in yeast.
34147122	10	53	theme	biomaterials	1658:1669	arg1	engineering					1622:1632	the engineering	1618:1632	the engineering of polysaccharide-based biomaterials that are needed for a sustainable bioeconomy	1618:1714	CONCLUSION The results provide novel routes for the engineering of polysaccharide-based biomaterials that are needed for a sustainable bioeconomy.
34147122	7	54	theme	glucomannan	1160:1170	arg1	synthase					1172:1179	an engineered glucomannan synthase	1146:1179	an engineered glucomannan synthase as well as CSLA pure mannan synthases and a CSLC glucan synthase	1146:1244	However, an engineered glucomannan synthase as well as CSLA pure mannan synthases and a CSLC glucan synthase did not inhibit growth.
34147122	8	55	theme	cell	1292:1295	arg1	size					1297:1300	Pichia cell size	1285:1300	Pichia cell size	1285:1300	Interestingly, Pichia cell size could be increased or decreased depending on the composition of the CSLA protein sequence.
34147122	10	56	theme	sustainable	1693:1703	arg1	bioeconomy					1705:1714	a sustainable bioeconomy	1691:1714	a sustainable bioeconomy	1691:1714	CONCLUSION The results provide novel routes for the engineering of polysaccharide-based biomaterials that are needed for a sustainable bioeconomy.
34147122	1	57	contain	have	142:145	arg2	humans					157:162	benefited humans	147:162	benefited humans for centuries	147:176	BACKGROUND The carbohydrate polymers that encapsulate plants cells have benefited humans for centuries and have valuable biotechnological uses.
34147122	1	57	contain	have	142:145	arg1	polymers					103:110	The carbohydrate polymers	86:110	The carbohydrate polymers that encapsulate plants cells	86:140	BACKGROUND The carbohydrate polymers that encapsulate plants cells have benefited humans for centuries and have valuable biotechnological uses.
34147122	9	58	from	thaliana	1560:1567	arg1	co-factor					1533:1541	a MANNAN-SYNTHESIS-RELATED (MSR) co-factor	1500:1541	a MANNAN-SYNTHESIS-RELATED (MSR) co-factor from Arabidopsis thaliana	1500:1567	HM yield and glucose incorporation could be further increased by co-expressing chimeric CSLA proteins with a MANNAN-SYNTHESIS-RELATED (MSR) co-factor from Arabidopsis thaliana.
34147122	2	59	theme	biomaterials	319:330	arg1	engineering					283:293	the engineering	279:293	the engineering of polysaccharide-based biomaterials	279:330	In the past 5 years, exciting possibilities have emerged in the engineering of polysaccharide-based biomaterials.
34147122	4	60	theme	heteromannan	677:688	arg1	polysaccharides					695:709	tunable heteromannan (HM) polysaccharides	669:709	tunable heteromannan (HM) polysaccharides	669:709	RESULTS Here, we assembled cellulose synthase-like A (CSLA) enzymes using an optimized Pichia pastoris platform to produce tunable heteromannan (HM) polysaccharides in yeast.
34147122	5	61	theme	mannan	754:759	arg1	domains					737:743	the domains	733:743	the domains of plant mannan and glucomannan synthases	733:785	By swapping the domains of plant mannan and glucomannan synthases, we engineered chimeric CSLA proteins that made β-1,4-linked mannan in quantities surpassing those of the native enzymes while minimizing the burden on yeast growth.
34147122	5	61	theme	mannan	754:759	arg1	mannan					754:759	plant mannan and glucomannan synthases	748:785	mannan	754:759	By swapping the domains of plant mannan and glucomannan synthases, we engineered chimeric CSLA proteins that made β-1,4-linked mannan in quantities surpassing those of the native enzymes while minimizing the burden on yeast growth.
34147122	5	61	theme	mannan	754:759	arg1	synthases					777:785	plant mannan and glucomannan synthases	748:785	synthases	777:785	By swapping the domains of plant mannan and glucomannan synthases, we engineered chimeric CSLA proteins that made β-1,4-linked mannan in quantities surpassing those of the native enzymes while minimizing the burden on yeast growth.
34147122	5	62	link	β-1,4-linked	835:846	arg1	mannan					848:853	β-1,4-linked mannan	835:853	β-1,4-linked mannan	835:853	By swapping the domains of plant mannan and glucomannan synthases, we engineered chimeric CSLA proteins that made β-1,4-linked mannan in quantities surpassing those of the native enzymes while minimizing the burden on yeast growth.
34147122	11	63	from	characterization	1721:1736	arg1	yeast					1785:1789	yeast	1785:1789	yeast	1785:1789	The characterization of chimeric cellulose synthase-like enzymes in yeast offers an exciting avenue to produce plant polysaccharides in a tunable manner.
34147122	5	64	theme	native	893:898	arg1	enzymes					900:906	the native enzymes	889:906	the native enzymes	889:906	By swapping the domains of plant mannan and glucomannan synthases, we engineered chimeric CSLA proteins that made β-1,4-linked mannan in quantities surpassing those of the native enzymes while minimizing the burden on yeast growth.
34147122	6	65	theme	Amorphophallus	1005:1018	arg1	konjac					1020:1025	Amorphophallus konjac	1005:1025	Amorphophallus konjac	1005:1025	Prolonged expression of a glucomannan synthase from Amorphophallus konjac was toxic to yeast cells: reducing biomass accumulation and ultimately leading to compromised cell viability.
34147122	2	66	theme	past	226:229	arg1	5 years					231:237	the past 5 years	222:237	the past 5 years	222:237	In the past 5 years, exciting possibilities have emerged in the engineering of polysaccharide-based biomaterials.
34147122	6	67	theme	synthase	991:998	arg1	expression					963:972	Prolonged expression	953:972	Prolonged expression of a glucomannan synthase from Amorphophallus konjac	953:1025	Prolonged expression of a glucomannan synthase from Amorphophallus konjac was toxic to yeast cells: reducing biomass accumulation and ultimately leading to compromised cell viability.
34147122	3	68	from	advances	352:359	arg1	hydrogels					390:398	bacterial cellulose-based hydrogels	364:398	bacterial cellulose-based hydrogels	364:398	Despite impressive advances on bacterial cellulose-based hydrogels, comparatively little is known about how plant hemicelluloses can be reconstituted and modulated in cells suitable for biotechnological purposes.
34147122	1	69	theme	plants	129:134	arg1	cells					136:140	plants cells	129:140	plants cells	129:140	BACKGROUND The carbohydrate polymers that encapsulate plants cells have benefited humans for centuries and have valuable biotechnological uses.
34147122	0	70	theme	yeast	62:66	arg1	cells					68:72	yeast cells	62:72	yeast cells	62:72	Modular biosynthesis of plant hemicellulose and its impact on yeast cells.
34147122	5	71	from	burden	929:934	arg1	growth					945:950	yeast growth	939:950	yeast growth	939:950	By swapping the domains of plant mannan and glucomannan synthases, we engineered chimeric CSLA proteins that made β-1,4-linked mannan in quantities surpassing those of the native enzymes while minimizing the burden on yeast growth.
34147122	3	72	theme	bacterial	364:372	arg1	hydrogels					390:398	bacterial cellulose-based hydrogels	364:398	bacterial cellulose-based hydrogels	364:398	Despite impressive advances on bacterial cellulose-based hydrogels, comparatively little is known about how plant hemicelluloses can be reconstituted and modulated in cells suitable for biotechnological purposes.
34147122	6	73	dep	cells	1046:1050	arg1	leading					1098:1104	leading	1098:1104	ultimately leading to compromised cell viability	1087:1134	Prolonged expression of a glucomannan synthase from Amorphophallus konjac was toxic to yeast cells: reducing biomass accumulation and ultimately leading to compromised cell viability.
34147122	6	73	dep	cells	1046:1050	arg1	reducing					1053:1060	reducing	1053:1060	reducing biomass accumulation	1053:1081	Prolonged expression of a glucomannan synthase from Amorphophallus konjac was toxic to yeast cells: reducing biomass accumulation and ultimately leading to compromised cell viability.
34147122	11	74	theme	tunable	1855:1861	arg1	manner					1863:1868	a tunable manner	1853:1868	a tunable manner	1853:1868	The characterization of chimeric cellulose synthase-like enzymes in yeast offers an exciting avenue to produce plant polysaccharides in a tunable manner.
34147122	5	75	theme	CSLA	811:814	arg1	proteins					816:823	chimeric CSLA proteins	802:823	chimeric CSLA proteins that made β-1,4-linked mannan in quantities surpassing those of the native enzymes while minimizing the burden on yeast growth	802:950	By swapping the domains of plant mannan and glucomannan synthases, we engineered chimeric CSLA proteins that made β-1,4-linked mannan in quantities surpassing those of the native enzymes while minimizing the burden on yeast growth.
34147122	0	76	from	biosynthesis	8:19	arg1	cells					68:72	yeast cells	62:72	yeast cells	62:72	Modular biosynthesis of plant hemicellulose and its impact on yeast cells.
34147122	8	77	theme	protein	1375:1381	arg1	sequence					1383:1390	the CSLA protein sequence	1366:1390	the CSLA protein sequence	1366:1390	Interestingly, Pichia cell size could be increased or decreased depending on the composition of the CSLA protein sequence.
34147122	5	78	theme	glucomannan	765:775	arg1	synthases					777:785	plant mannan and glucomannan synthases	748:785	synthases	777:785	By swapping the domains of plant mannan and glucomannan synthases, we engineered chimeric CSLA proteins that made β-1,4-linked mannan in quantities surpassing those of the native enzymes while minimizing the burden on yeast growth.
34147122	0	79	from	impact	52:57	arg1	cells					68:72	yeast cells	62:72	yeast cells	62:72	Modular biosynthesis of plant hemicellulose and its impact on yeast cells.
34147122	7	80	dep	mannan	1202:1207	arg1	synthases					1209:1217	synthases	1209:1217	synthases	1209:1217	However, an engineered glucomannan synthase as well as CSLA pure mannan synthases and a CSLC glucan synthase did not inhibit growth.
34147122	7	81	theme	glucan	1230:1235	arg1	synthase					1237:1244	CSLA pure mannan synthases and a CSLC glucan synthase	1192:1244	an engineered glucomannan synthase as well as CSLA pure mannan synthases and a CSLC glucan synthase	1146:1244	However, an engineered glucomannan synthase as well as CSLA pure mannan synthases and a CSLC glucan synthase did not inhibit growth.
34147122	12	82	theme	therapeutic	2028:2038	arg1	proteins					2040:2047	therapeutic proteins	2028:2047	therapeutic proteins	2028:2047	Furthermore, cells modified with non-toxic plant polysaccharides such as β-mannan offer a modular chassis to produce and encapsulate sensitive cargo such as therapeutic proteins.
34147122	0	83	theme	plant	24:28	arg1	hemicellulose					30:42	plant hemicellulose	24:42	plant hemicellulose	24:42	Modular biosynthesis of plant hemicellulose and its impact on yeast cells.
34147122	9	84	theme	HM	1393:1394	arg1	yield					1396:1400	HM yield	1393:1400	HM yield	1393:1400	HM yield and glucose incorporation could be further increased by co-expressing chimeric CSLA proteins with a MANNAN-SYNTHESIS-RELATED (MSR) co-factor from Arabidopsis thaliana.
34147122	1	85	theme	carbohydrate	90:101	arg1	polymers					103:110	The carbohydrate polymers	86:110	The carbohydrate polymers that encapsulate plants cells	86:140	BACKGROUND The carbohydrate polymers that encapsulate plants cells have benefited humans for centuries and have valuable biotechnological uses.
34147122	6	86	theme	cell	1121:1124	arg1	viability					1126:1134	compromised cell viability	1109:1134	compromised cell viability	1109:1134	Prolonged expression of a glucomannan synthase from Amorphophallus konjac was toxic to yeast cells: reducing biomass accumulation and ultimately leading to compromised cell viability.
34147122	6	87	from	konjac	1020:1025	arg1	expression					963:972	Prolonged expression	953:972	Prolonged expression of a glucomannan synthase from Amorphophallus konjac	953:1025	Prolonged expression of a glucomannan synthase from Amorphophallus konjac was toxic to yeast cells: reducing biomass accumulation and ultimately leading to compromised cell viability.
34147122	6	87	from	konjac	1020:1025	arg1	synthase					991:998	a glucomannan synthase	977:998	a glucomannan synthase from Amorphophallus konjac	977:1025	Prolonged expression of a glucomannan synthase from Amorphophallus konjac was toxic to yeast cells: reducing biomass accumulation and ultimately leading to compromised cell viability.
34147122	12	88	theme	sensitive	2004:2012	arg1	cargo					2014:2018	sensitive cargo	2004:2018	sensitive cargo such as therapeutic proteins	2004:2047	Furthermore, cells modified with non-toxic plant polysaccharides such as β-mannan offer a modular chassis to produce and encapsulate sensitive cargo such as therapeutic proteins.
34147122	12	88	theme	sensitive	2004:2012	arg1	proteins					2040:2047	therapeutic proteins	2028:2047	therapeutic proteins	2028:2047	Furthermore, cells modified with non-toxic plant polysaccharides such as β-mannan offer a modular chassis to produce and encapsulate sensitive cargo such as therapeutic proteins.
34147122	4	89	theme	synthase-like	583:595	arg1	A					597:597	cellulose synthase-like A	573:597	cellulose synthase-like A (CSLA) enzymes	573:612	RESULTS Here, we assembled cellulose synthase-like A (CSLA) enzymes using an optimized Pichia pastoris platform to produce tunable heteromannan (HM) polysaccharides in yeast.
34147122	4	89	theme	synthase-like	583:595	arg1	CSLA					600:603	CSLA	600:603	CSLA	600:603	RESULTS Here, we assembled cellulose synthase-like A (CSLA) enzymes using an optimized Pichia pastoris platform to produce tunable heteromannan (HM) polysaccharides in yeast.
34147122	7	90	theme	pure	1197:1200	arg1	mannan					1202:1207	CSLA pure mannan synthases	1192:1217	CSLA pure mannan synthases	1192:1217	However, an engineered glucomannan synthase as well as CSLA pure mannan synthases and a CSLC glucan synthase did not inhibit growth.
34147122	12	91	theme	modular	1961:1967	arg1	chassis					1969:1975	a modular chassis	1959:1975	a modular chassis to produce and encapsulate sensitive cargo such as therapeutic proteins	1959:2047	Furthermore, cells modified with non-toxic plant polysaccharides such as β-mannan offer a modular chassis to produce and encapsulate sensitive cargo such as therapeutic proteins.
34147122	3	92	theme	plant	441:445	arg1	hemicelluloses					447:460	plant hemicelluloses	441:460	plant hemicelluloses	441:460	Despite impressive advances on bacterial cellulose-based hydrogels, comparatively little is known about how plant hemicelluloses can be reconstituted and modulated in cells suitable for biotechnological purposes.
34147122	10	93	theme	novel	1601:1605	arg1	routes					1607:1612	novel routes	1601:1612	novel routes for the engineering of polysaccharide-based biomaterials that are needed for a sustainable bioeconomy	1601:1714	CONCLUSION The results provide novel routes for the engineering of polysaccharide-based biomaterials that are needed for a sustainable bioeconomy.
34147122	4	94	theme	tunable	669:675	arg1	HM					691:692	HM	691:692	HM	691:692	RESULTS Here, we assembled cellulose synthase-like A (CSLA) enzymes using an optimized Pichia pastoris platform to produce tunable heteromannan (HM) polysaccharides in yeast.
34147122	4	94	theme	tunable	669:675	arg1	heteromannan					677:688	tunable heteromannan	669:688	tunable heteromannan (HM) polysaccharides	669:709	RESULTS Here, we assembled cellulose synthase-like A (CSLA) enzymes using an optimized Pichia pastoris platform to produce tunable heteromannan (HM) polysaccharides in yeast.
34147122	11	95	theme	cellulose	1750:1758	arg1	enzymes					1774:1780	chimeric cellulose synthase-like enzymes	1741:1780	chimeric cellulose synthase-like enzymes in yeast	1741:1789	The characterization of chimeric cellulose synthase-like enzymes in yeast offers an exciting avenue to produce plant polysaccharides in a tunable manner.
33712885	0	0	theme	Pathophysiological	74:91	arg1	Changes					0:6	Changes	0:6	Changes in the Brain Extracellular Matrix Composition in schizophrenia: A Pathophysiological Dysregulation and a Potential Therapeutic Target.	0:141	Changes in the Brain Extracellular Matrix Composition in schizophrenia: A Pathophysiological Dysregulation and a Potential Therapeutic Target.
33712885	0	0	theme	Pathophysiological	74:91	arg1	Dysregulation					93:105	A Pathophysiological Dysregulation	72:105	A Pathophysiological Dysregulation	72:105	Changes in the Brain Extracellular Matrix Composition in schizophrenia: A Pathophysiological Dysregulation and a Potential Therapeutic Target.
33712885	6	1	theme	proteins	930:937	arg1	distribution					900:911	the distribution	896:911	the distribution of the brain ECM proteins by confocal microscopy	896:960	Lastly, immunostaining of post-mortem human brain tissue was performed to analyse the distribution of the brain ECM proteins by confocal microscopy.
33712885	7	2	theme	brain	1113:1117	arg1	ECM					1119:1121	the brain ECM	1109:1121	the brain ECM	1109:1121	The analysis identified four proteins: fibronectin, lumican, nidogen-1, and secreted protein acidic and rich in cysteine (SPARC) as components of the brain ECM.
33712885	10	3	theme	proteins	1460:1467	arg1	dysregulation					1443:1455	a pathophysiological dysregulation	1422:1455	a pathophysiological dysregulation	1422:1455	Our study proposes a pathophysiological dysregulation of proteins of the brain ECM, whose abnormal composition leads to a progressive neuronal impairment and consequently to neurodegenerative processes due to lack of neurophysiological support and dysregulation of neuronal homeostasis.
33712885	3	4	theme	dynamic	523:529	arg1	SZ					534:535	its dynamic in SZ	519:535	its dynamic in SZ	519:535	The characterization of the brain ECM is critical to understand its dynamic in SZ.
33712885	7	5	from	rich	1067:1070	arg1	cysteine					1075:1082	cysteine	1075:1082	cysteine	1075:1082	The analysis identified four proteins: fibronectin, lumican, nidogen-1, and secreted protein acidic and rich in cysteine (SPARC) as components of the brain ECM.
33712885	5	6	theme	classical	783:791	arg1	method					806:811	the classical western blot method	779:811	the classical western blot method	779:811	Plasma of participants was analysed by label-free liquid chromatography-tandem mass spectrometry, and the results were validated using the classical western blot method.
33712885	6	7	theme	ECM	926:928	arg1	proteins					930:937	the brain ECM proteins	916:937	the brain ECM proteins	916:937	Lastly, immunostaining of post-mortem human brain tissue was performed to analyse the distribution of the brain ECM proteins by confocal microscopy.
33712885	7	8	theme	ECM	1119:1121	arg1	components					1095:1104	components	1095:1104	components of the brain ECM	1095:1121	The analysis identified four proteins: fibronectin, lumican, nidogen-1, and secreted protein acidic and rich in cysteine (SPARC) as components of the brain ECM.
33712885	7	8	theme	ECM	1119:1121	arg1	proteins					992:999	four proteins	987:999	four proteins: fibronectin, lumican, nidogen-1, and secreted protein acidic and rich in cysteine (SPARC)	987:1090	The analysis identified four proteins: fibronectin, lumican, nidogen-1, and secreted protein acidic and rich in cysteine (SPARC) as components of the brain ECM.
33712885	3	9	theme	in	531:532	arg1	SZ					534:535	its dynamic in SZ	519:535	its dynamic in SZ	519:535	The characterization of the brain ECM is critical to understand its dynamic in SZ.
33712885	10	10	theme	neuronal	1668:1675	arg1	homeostasis					1677:1687	neuronal homeostasis	1668:1687	neuronal homeostasis	1668:1687	Our study proposes a pathophysiological dysregulation of proteins of the brain ECM, whose abnormal composition leads to a progressive neuronal impairment and consequently to neurodegenerative processes due to lack of neurophysiological support and dysregulation of neuronal homeostasis.
33712885	1	11	theme	synaptic	245:252	arg1	plasticity					254:263	neuronal and synaptic plasticity	232:263	plasticity	254:263	The brain extracellular matrix (ECM) is involved in crucial processes of neural support, neuronal and synaptic plasticity, extrasynaptic transmission, and neurotransmission.
33712885	11	12	theme	pharmacological	1747:1761	arg1	ECM					1710:1712	the brain ECM	1700:1712	the brain ECM	1700:1712	Moreover, the brain ECM and its components are potential pharmacological targets to develop new therapeutic approaches to treat SZ.
33712885	11	12	theme	pharmacological	1747:1761	arg1	components					1722:1731	its components	1718:1731	its components	1718:1731	Moreover, the brain ECM and its components are potential pharmacological targets to develop new therapeutic approaches to treat SZ.
33712885	11	12	theme	pharmacological	1747:1761	arg1	targets					1763:1769	potential pharmacological targets	1737:1769	potential pharmacological targets	1737:1769	Moreover, the brain ECM and its components are potential pharmacological targets to develop new therapeutic approaches to treat SZ.
33712885	0	13	from	Changes	0:6	arg1	schizophrenia					57:69	schizophrenia	57:69	schizophrenia	57:69	Changes in the Brain Extracellular Matrix Composition in schizophrenia: A Pathophysiological Dysregulation and a Potential Therapeutic Target.
33712885	0	13	from	Changes	0:6	arg1	Composition					42:52	the Brain Extracellular Matrix Composition	11:52	the Brain Extracellular Matrix Composition	11:52	Changes in the Brain Extracellular Matrix Composition in schizophrenia: A Pathophysiological Dysregulation and a Potential Therapeutic Target.
33712885	5	14	theme	participants	654:665	arg1	Plasma					644:649	Plasma	644:649	Plasma of participants	644:665	Plasma of participants was analysed by label-free liquid chromatography-tandem mass spectrometry, and the results were validated using the classical western blot method.
33712885	0	15	dep	Potential	113:121	arg1	Therapeutic					123:133	Therapeutic	123:133	Therapeutic	123:133	Changes in the Brain Extracellular Matrix Composition in schizophrenia: A Pathophysiological Dysregulation and a Potential Therapeutic Target.
33712885	1	16	theme	plasticity	254:263	arg1	processes					203:211	crucial processes	195:211	crucial processes of neural support, neuronal and synaptic plasticity, extrasynaptic transmission, and neurotransmission	195:314	The brain extracellular matrix (ECM) is involved in crucial processes of neural support, neuronal and synaptic plasticity, extrasynaptic transmission, and neurotransmission.
33712885	8	17	theme	SZ	1285:1286	arg1	group					1288:1292	the SZ group	1281:1292	the SZ group	1281:1292	Statistical significance was found for fibronectin (P = 0.0166), SPARC (P = 0.0003), lumican (P = 0.0012), and nidogen-1 (P < 0.0001) that were decreased in the SZ group.
33712885	9	18	theme	prefrontal	1319:1328	arg1	PFC					1338:1340	PFC	1338:1340	PFC	1338:1340	Fluorescence imaging of prefrontal cortex (PFC) sections revealed a lower expression of ECM proteins in SZ.
33712885	9	18	theme	prefrontal	1319:1328	arg1	cortex					1330:1335	prefrontal cortex	1319:1335	prefrontal cortex (PFC) sections	1319:1350	Fluorescence imaging of prefrontal cortex (PFC) sections revealed a lower expression of ECM proteins in SZ.
33712885	11	19	theme	potential	1737:1745	arg1	ECM					1710:1712	the brain ECM	1700:1712	the brain ECM	1700:1712	Moreover, the brain ECM and its components are potential pharmacological targets to develop new therapeutic approaches to treat SZ.
33712885	11	19	theme	potential	1737:1745	arg1	components					1722:1731	its components	1718:1731	its components	1718:1731	Moreover, the brain ECM and its components are potential pharmacological targets to develop new therapeutic approaches to treat SZ.
33712885	11	19	theme	potential	1737:1745	arg1	targets					1763:1769	potential pharmacological targets	1737:1769	potential pharmacological targets	1737:1769	Moreover, the brain ECM and its components are potential pharmacological targets to develop new therapeutic approaches to treat SZ.
33712885	0	20	theme	Potential	113:121	arg1	Changes					0:6	Changes	0:6	Changes in the Brain Extracellular Matrix Composition in schizophrenia: A Pathophysiological Dysregulation and a Potential Therapeutic Target.	0:141	Changes in the Brain Extracellular Matrix Composition in schizophrenia: A Pathophysiological Dysregulation and a Potential Therapeutic Target.
33712885	0	20	theme	Potential	113:121	arg1	Target					135:140	a Potential Therapeutic Target	111:140	a Potential Therapeutic Target	111:140	Changes in the Brain Extracellular Matrix Composition in schizophrenia: A Pathophysiological Dysregulation and a Potential Therapeutic Target.
33712885	1	21	theme	crucial	195:201	arg1	processes					203:211	crucial processes	195:211	crucial processes of neural support, neuronal and synaptic plasticity, extrasynaptic transmission, and neurotransmission	195:314	The brain extracellular matrix (ECM) is involved in crucial processes of neural support, neuronal and synaptic plasticity, extrasynaptic transmission, and neurotransmission.
33712885	9	22	theme	ECM	1383:1385	arg1	proteins					1387:1394	ECM proteins	1383:1394	ECM proteins	1383:1394	Fluorescence imaging of prefrontal cortex (PFC) sections revealed a lower expression of ECM proteins in SZ.
33712885	9	23	theme	lower	1363:1367	arg1	expression					1369:1378	a lower expression	1361:1378	a lower expression of ECM proteins in SZ	1361:1400	Fluorescence imaging of prefrontal cortex (PFC) sections revealed a lower expression of ECM proteins in SZ.
33712885	5	24	theme	western	793:799	arg1	method					806:811	the classical western blot method	779:811	the classical western blot method	779:811	Plasma of participants was analysed by label-free liquid chromatography-tandem mass spectrometry, and the results were validated using the classical western blot method.
33712885	9	25	theme	cortex	1330:1335	arg1	sections					1343:1350	prefrontal cortex (PFC) sections	1319:1350	prefrontal cortex (PFC) sections	1319:1350	Fluorescence imaging of prefrontal cortex (PFC) sections revealed a lower expression of ECM proteins in SZ.
33712885	10	26	theme	ECM	1482:1484	arg1	proteins					1460:1467	proteins	1460:1467	proteins of the brain ECM	1460:1484	Our study proposes a pathophysiological dysregulation of proteins of the brain ECM, whose abnormal composition leads to a progressive neuronal impairment and consequently to neurodegenerative processes due to lack of neurophysiological support and dysregulation of neuronal homeostasis.
33712885	10	27	theme	abnormal	1493:1500	arg1	composition					1502:1512	composition	1502:1512	composition	1502:1512	Our study proposes a pathophysiological dysregulation of proteins of the brain ECM, whose abnormal composition leads to a progressive neuronal impairment and consequently to neurodegenerative processes due to lack of neurophysiological support and dysregulation of neuronal homeostasis.
33712885	1	28	theme	extrasynaptic	266:278	arg1	transmission					280:291	extrasynaptic transmission	266:291	extrasynaptic transmission	266:291	The brain extracellular matrix (ECM) is involved in crucial processes of neural support, neuronal and synaptic plasticity, extrasynaptic transmission, and neurotransmission.
33712885	11	29	theme	brain	1704:1708	arg1	ECM					1710:1712	the brain ECM	1700:1712	the brain ECM	1700:1712	Moreover, the brain ECM and its components are potential pharmacological targets to develop new therapeutic approaches to treat SZ.
33712885	11	29	theme	brain	1704:1708	arg1	components					1722:1731	its components	1718:1731	its components	1718:1731	Moreover, the brain ECM and its components are potential pharmacological targets to develop new therapeutic approaches to treat SZ.
33712885	11	29	theme	brain	1704:1708	arg1	targets					1763:1769	potential pharmacological targets	1737:1769	potential pharmacological targets	1737:1769	Moreover, the brain ECM and its components are potential pharmacological targets to develop new therapeutic approaches to treat SZ.
33712885	10	30	theme	due	1605:1607	arg1	processes					1595:1603	neurodegenerative processes	1577:1603	neurodegenerative processes due to lack of neurophysiological support and dysregulation of neuronal homeostasis	1577:1687	Our study proposes a pathophysiological dysregulation of proteins of the brain ECM, whose abnormal composition leads to a progressive neuronal impairment and consequently to neurodegenerative processes due to lack of neurophysiological support and dysregulation of neuronal homeostasis.
33712885	0	31	theme	Brain	15:19	arg1	Composition					42:52	the Brain Extracellular Matrix Composition	11:52	the Brain Extracellular Matrix Composition	11:52	Changes in the Brain Extracellular Matrix Composition in schizophrenia: A Pathophysiological Dysregulation and a Potential Therapeutic Target.
33712885	1	32	theme	transmission	280:291	arg1	processes					203:211	crucial processes	195:211	crucial processes of neural support, neuronal and synaptic plasticity, extrasynaptic transmission, and neurotransmission	195:314	The brain extracellular matrix (ECM) is involved in crucial processes of neural support, neuronal and synaptic plasticity, extrasynaptic transmission, and neurotransmission.
33712885	5	33	theme	chromatography-tandem	701:721	arg1	spectrometry					728:739	label-free liquid chromatography-tandem mass spectrometry	683:739	label-free liquid chromatography-tandem mass spectrometry	683:739	Plasma of participants was analysed by label-free liquid chromatography-tandem mass spectrometry, and the results were validated using the classical western blot method.
33712885	3	34	theme	ECM	489:491	arg1	characterization					459:474	The characterization	455:474	The characterization of the brain ECM	455:491	The characterization of the brain ECM is critical to understand its dynamic in SZ.
33712885	3	34	theme	ECM	489:491	arg1	critical					496:503	critical	496:503	critical	496:503	The characterization of the brain ECM is critical to understand its dynamic in SZ.
33712885	10	35	theme	progressive	1525:1535	arg1	impairment					1546:1555	a progressive neuronal impairment	1523:1555	a progressive neuronal impairment	1523:1555	Our study proposes a pathophysiological dysregulation of proteins of the brain ECM, whose abnormal composition leads to a progressive neuronal impairment and consequently to neurodegenerative processes due to lack of neurophysiological support and dysregulation of neuronal homeostasis.
33712885	9	36	theme	Fluorescence	1295:1306	arg1	imaging					1308:1314	Fluorescence imaging	1295:1314	Fluorescence imaging of prefrontal cortex (PFC) sections	1295:1350	Fluorescence imaging of prefrontal cortex (PFC) sections revealed a lower expression of ECM proteins in SZ.
33712885	5	37	theme	mass	723:726	arg1	spectrometry					728:739	label-free liquid chromatography-tandem mass spectrometry	683:739	label-free liquid chromatography-tandem mass spectrometry	683:739	Plasma of participants was analysed by label-free liquid chromatography-tandem mass spectrometry, and the results were validated using the classical western blot method.
33712885	2	38	theme	unique	431:436	arg1	characteristics					438:452	unique characteristics	431:452	it unique characteristics	428:452	ECM is a tridimensional fibrillary meshwork composed of macromolecules that determine its bioactivity and give it unique characteristics.
33712885	0	39	theme	Matrix	35:40	arg1	Composition					42:52	the Brain Extracellular Matrix Composition	11:52	the Brain Extracellular Matrix Composition	11:52	Changes in the Brain Extracellular Matrix Composition in schizophrenia: A Pathophysiological Dysregulation and a Potential Therapeutic Target.
33712885	10	40	theme	homeostasis	1677:1687	arg1	support					1639:1645	neurophysiological support	1620:1645	neurophysiological support	1620:1645	Our study proposes a pathophysiological dysregulation of proteins of the brain ECM, whose abnormal composition leads to a progressive neuronal impairment and consequently to neurodegenerative processes due to lack of neurophysiological support and dysregulation of neuronal homeostasis.
33712885	10	40	theme	homeostasis	1677:1687	arg1	dysregulation					1651:1663	dysregulation	1651:1663	dysregulation of neuronal homeostasis	1651:1687	Our study proposes a pathophysiological dysregulation of proteins of the brain ECM, whose abnormal composition leads to a progressive neuronal impairment and consequently to neurodegenerative processes due to lack of neurophysiological support and dysregulation of neuronal homeostasis.
33712885	10	41	theme	pathophysiological	1424:1441	arg1	dysregulation					1443:1455	a pathophysiological dysregulation	1422:1455	a pathophysiological dysregulation	1422:1455	Our study proposes a pathophysiological dysregulation of proteins of the brain ECM, whose abnormal composition leads to a progressive neuronal impairment and consequently to neurodegenerative processes due to lack of neurophysiological support and dysregulation of neuronal homeostasis.
33712885	2	42	dep	it	428:429	arg1	characteristics					438:452	unique characteristics	431:452	it unique characteristics	428:452	ECM is a tridimensional fibrillary meshwork composed of macromolecules that determine its bioactivity and give it unique characteristics.
33712885	10	43	theme	support	1639:1645	arg1	lack					1612:1615	lack	1612:1615	lack of neurophysiological support and dysregulation of neuronal homeostasis	1612:1687	Our study proposes a pathophysiological dysregulation of proteins of the brain ECM, whose abnormal composition leads to a progressive neuronal impairment and consequently to neurodegenerative processes due to lack of neurophysiological support and dysregulation of neuronal homeostasis.
33712885	0	44	theme	Extracellular	21:33	arg1	Composition					42:52	the Brain Extracellular Matrix Composition	11:52	the Brain Extracellular Matrix Composition	11:52	Changes in the Brain Extracellular Matrix Composition in schizophrenia: A Pathophysiological Dysregulation and a Potential Therapeutic Target.
33712885	1	45	theme	neurotransmission	298:314	arg1	processes					203:211	crucial processes	195:211	crucial processes of neural support, neuronal and synaptic plasticity, extrasynaptic transmission, and neurotransmission	195:314	The brain extracellular matrix (ECM) is involved in crucial processes of neural support, neuronal and synaptic plasticity, extrasynaptic transmission, and neurotransmission.
33712885	5	46	theme	blot	801:804	arg1	method					806:811	the classical western blot method	779:811	the classical western blot method	779:811	Plasma of participants was analysed by label-free liquid chromatography-tandem mass spectrometry, and the results were validated using the classical western blot method.
33712885	10	47	theme	neuronal	1537:1544	arg1	impairment					1546:1555	a progressive neuronal impairment	1523:1555	a progressive neuronal impairment	1523:1555	Our study proposes a pathophysiological dysregulation of proteins of the brain ECM, whose abnormal composition leads to a progressive neuronal impairment and consequently to neurodegenerative processes due to lack of neurophysiological support and dysregulation of neuronal homeostasis.
33712885	3	48	theme	brain	483:487	arg1	ECM					489:491	the brain ECM	479:491	the brain ECM	479:491	The characterization of the brain ECM is critical to understand its dynamic in SZ.
33712885	9	49	theme	proteins	1387:1394	arg1	expression					1369:1378	a lower expression	1361:1378	a lower expression of ECM proteins in SZ	1361:1400	Fluorescence imaging of prefrontal cortex (PFC) sections revealed a lower expression of ECM proteins in SZ.
33712885	10	50	theme	brain	1476:1480	arg1	ECM					1482:1484	the brain ECM	1472:1484	the brain ECM	1472:1484	Our study proposes a pathophysiological dysregulation of proteins of the brain ECM, whose abnormal composition leads to a progressive neuronal impairment and consequently to neurodegenerative processes due to lack of neurophysiological support and dysregulation of neuronal homeostasis.
33712885	11	51	theme	therapeutic	1786:1796	arg1	approaches					1798:1807	new therapeutic approaches	1782:1807	new therapeutic approaches	1782:1807	Moreover, the brain ECM and its components are potential pharmacological targets to develop new therapeutic approaches to treat SZ.
33712885	6	52	theme	brain	920:924	arg1	proteins					930:937	the brain ECM proteins	916:937	the brain ECM proteins	916:937	Lastly, immunostaining of post-mortem human brain tissue was performed to analyse the distribution of the brain ECM proteins by confocal microscopy.
33712885	4	53	theme	comparative	546:556	arg1	study					558:562	a comparative study	544:562	a comparative study	544:562	Thus, a comparative study was developed with 71 patients with schizophrenia (SZ) and 70 healthy controls.
33712885	9	54	from	expression	1369:1378	arg1	SZ					1399:1400	SZ	1399:1400	SZ	1399:1400	Fluorescence imaging of prefrontal cortex (PFC) sections revealed a lower expression of ECM proteins in SZ.
33712885	6	55	theme	tissue	864:869	arg1	immunostaining					822:835	immunostaining	822:835	immunostaining of post-mortem human brain tissue	822:869	Lastly, immunostaining of post-mortem human brain tissue was performed to analyse the distribution of the brain ECM proteins by confocal microscopy.
33712885	6	56	theme	brain	858:862	arg1	tissue					864:869	post-mortem human brain tissue	840:869	post-mortem human brain tissue	840:869	Lastly, immunostaining of post-mortem human brain tissue was performed to analyse the distribution of the brain ECM proteins by confocal microscopy.
33712885	2	57	theme	fibrillary	341:350	arg1	meshwork					352:359	a tridimensional fibrillary meshwork	324:359	a tridimensional fibrillary meshwork composed of macromolecules that determine its bioactivity and give it unique characteristics	324:452	ECM is a tridimensional fibrillary meshwork composed of macromolecules that determine its bioactivity and give it unique characteristics.
33712885	2	57	theme	fibrillary	341:350	arg1	ECM					317:319	ECM	317:319	ECM	317:319	ECM is a tridimensional fibrillary meshwork composed of macromolecules that determine its bioactivity and give it unique characteristics.
33712885	6	58	theme	confocal	942:949	arg1	microscopy					951:960	confocal microscopy	942:960	confocal microscopy	942:960	Lastly, immunostaining of post-mortem human brain tissue was performed to analyse the distribution of the brain ECM proteins by confocal microscopy.
33712885	5	59	theme	label-free	683:692	arg1	spectrometry					728:739	label-free liquid chromatography-tandem mass spectrometry	683:739	label-free liquid chromatography-tandem mass spectrometry	683:739	Plasma of participants was analysed by label-free liquid chromatography-tandem mass spectrometry, and the results were validated using the classical western blot method.
33712885	4	60	with	controls	634:641	arg1	SZ					615:616	SZ	615:616	SZ	615:616	Thus, a comparative study was developed with 71 patients with schizophrenia (SZ) and 70 healthy controls.
33712885	4	60	with	controls	634:641	arg1	schizophrenia					600:612	schizophrenia	600:612	schizophrenia (SZ)	600:617	Thus, a comparative study was developed with 71 patients with schizophrenia (SZ) and 70 healthy controls.
33712885	11	61	theme	new	1782:1784	arg1	approaches					1798:1807	new therapeutic approaches	1782:1807	new therapeutic approaches	1782:1807	Moreover, the brain ECM and its components are potential pharmacological targets to develop new therapeutic approaches to treat SZ.
33712885	2	62	theme	tridimensional	326:339	arg1	meshwork					352:359	a tridimensional fibrillary meshwork	324:359	a tridimensional fibrillary meshwork composed of macromolecules that determine its bioactivity and give it unique characteristics	324:452	ECM is a tridimensional fibrillary meshwork composed of macromolecules that determine its bioactivity and give it unique characteristics.
33712885	2	62	theme	tridimensional	326:339	arg1	ECM					317:319	ECM	317:319	ECM	317:319	ECM is a tridimensional fibrillary meshwork composed of macromolecules that determine its bioactivity and give it unique characteristics.
33712885	1	63	theme	neural	216:221	arg1	support					223:229	neural support	216:229	neural support	216:229	The brain extracellular matrix (ECM) is involved in crucial processes of neural support, neuronal and synaptic plasticity, extrasynaptic transmission, and neurotransmission.
33712885	5	64	theme	liquid	694:699	arg1	spectrometry					728:739	label-free liquid chromatography-tandem mass spectrometry	683:739	label-free liquid chromatography-tandem mass spectrometry	683:739	Plasma of participants was analysed by label-free liquid chromatography-tandem mass spectrometry, and the results were validated using the classical western blot method.
33712885	6	65	theme	human	852:856	arg1	tissue					864:869	post-mortem human brain tissue	840:869	post-mortem human brain tissue	840:869	Lastly, immunostaining of post-mortem human brain tissue was performed to analyse the distribution of the brain ECM proteins by confocal microscopy.
33712885	10	66	theme	neurophysiological	1620:1637	arg1	support					1639:1645	neurophysiological support	1620:1645	neurophysiological support	1620:1645	Our study proposes a pathophysiological dysregulation of proteins of the brain ECM, whose abnormal composition leads to a progressive neuronal impairment and consequently to neurodegenerative processes due to lack of neurophysiological support and dysregulation of neuronal homeostasis.
33712885	10	67	theme	dysregulation	1651:1663	arg1	lack					1612:1615	lack	1612:1615	lack of neurophysiological support and dysregulation of neuronal homeostasis	1612:1687	Our study proposes a pathophysiological dysregulation of proteins of the brain ECM, whose abnormal composition leads to a progressive neuronal impairment and consequently to neurodegenerative processes due to lack of neurophysiological support and dysregulation of neuronal homeostasis.
33712885	1	68	theme	support	223:229	arg1	processes					203:211	crucial processes	195:211	crucial processes of neural support, neuronal and synaptic plasticity, extrasynaptic transmission, and neurotransmission	195:314	The brain extracellular matrix (ECM) is involved in crucial processes of neural support, neuronal and synaptic plasticity, extrasynaptic transmission, and neurotransmission.
33712885	8	69	theme	Statistical	1124:1134	arg1	significance					1136:1147	Statistical significance	1124:1147	Statistical significance	1124:1147	Statistical significance was found for fibronectin (P = 0.0166), SPARC (P = 0.0003), lumican (P = 0.0012), and nidogen-1 (P < 0.0001) that were decreased in the SZ group.
33712885	6	70	theme	post-mortem	840:850	arg1	tissue					864:869	post-mortem human brain tissue	840:869	post-mortem human brain tissue	840:869	Lastly, immunostaining of post-mortem human brain tissue was performed to analyse the distribution of the brain ECM proteins by confocal microscopy.
33712885	4	71	with	patients	586:593	arg1	SZ					615:616	SZ	615:616	SZ	615:616	Thus, a comparative study was developed with 71 patients with schizophrenia (SZ) and 70 healthy controls.
33712885	4	71	with	patients	586:593	arg1	schizophrenia					600:612	schizophrenia	600:612	schizophrenia (SZ)	600:617	Thus, a comparative study was developed with 71 patients with schizophrenia (SZ) and 70 healthy controls.
33712885	4	72	theme	healthy	626:632	arg1	controls					634:641	70 healthy controls	623:641	70 healthy controls	623:641	Thus, a comparative study was developed with 71 patients with schizophrenia (SZ) and 70 healthy controls.
33712885	1	73	theme	brain	147:151	arg1	ECM					175:177	ECM	175:177	ECM	175:177	The brain extracellular matrix (ECM) is involved in crucial processes of neural support, neuronal and synaptic plasticity, extrasynaptic transmission, and neurotransmission.
33712885	1	73	theme	brain	147:151	arg1	matrix					167:172	The brain extracellular matrix	143:172	The brain extracellular matrix (ECM)	143:178	The brain extracellular matrix (ECM) is involved in crucial processes of neural support, neuronal and synaptic plasticity, extrasynaptic transmission, and neurotransmission.
33712885	7	74	dep	proteins	992:999	arg1	lumican					1015:1021	lumican	1015:1021	lumican	1015:1021	The analysis identified four proteins: fibronectin, lumican, nidogen-1, and secreted protein acidic and rich in cysteine (SPARC) as components of the brain ECM.
33712885	7	74	dep	proteins	992:999	arg1	acidic					1056:1061	acidic	1056:1061	acidic	1056:1061	The analysis identified four proteins: fibronectin, lumican, nidogen-1, and secreted protein acidic and rich in cysteine (SPARC) as components of the brain ECM.
33712885	7	74	dep	proteins	992:999	arg1	SPARC					1085:1089	SPARC	1085:1089	SPARC	1085:1089	The analysis identified four proteins: fibronectin, lumican, nidogen-1, and secreted protein acidic and rich in cysteine (SPARC) as components of the brain ECM.
33712885	7	74	dep	proteins	992:999	arg1	proteins					992:999	four proteins	987:999	four proteins: fibronectin, lumican, nidogen-1, and secreted protein acidic and rich in cysteine (SPARC)	987:1090	The analysis identified four proteins: fibronectin, lumican, nidogen-1, and secreted protein acidic and rich in cysteine (SPARC) as components of the brain ECM.
33712885	7	74	dep	proteins	992:999	arg1	nidogen-1					1024:1032	nidogen-1	1024:1032	nidogen-1	1024:1032	The analysis identified four proteins: fibronectin, lumican, nidogen-1, and secreted protein acidic and rich in cysteine (SPARC) as components of the brain ECM.
33712885	7	74	dep	proteins	992:999	arg1	fibronectin					1002:1012	fibronectin	1002:1012	fibronectin	1002:1012	The analysis identified four proteins: fibronectin, lumican, nidogen-1, and secreted protein acidic and rich in cysteine (SPARC) as components of the brain ECM.
33712885	9	75	theme	sections	1343:1350	arg1	imaging					1308:1314	Fluorescence imaging	1295:1314	Fluorescence imaging of prefrontal cortex (PFC) sections	1295:1350	Fluorescence imaging of prefrontal cortex (PFC) sections revealed a lower expression of ECM proteins in SZ.
33712885	7	76	from	cysteine	1075:1082	arg1	rich					1067:1070	rich	1067:1070	rich	1067:1070	The analysis identified four proteins: fibronectin, lumican, nidogen-1, and secreted protein acidic and rich in cysteine (SPARC) as components of the brain ECM.
33712885	0	77	dep	Changes	0:6	arg1	Changes					0:6	Changes	0:6	Changes in the Brain Extracellular Matrix Composition in schizophrenia: A Pathophysiological Dysregulation and a Potential Therapeutic Target.	0:141	Changes in the Brain Extracellular Matrix Composition in schizophrenia: A Pathophysiological Dysregulation and a Potential Therapeutic Target.
33712885	0	77	dep	Changes	0:6	arg1	Target					135:140	a Potential Therapeutic Target	111:140	a Potential Therapeutic Target	111:140	Changes in the Brain Extracellular Matrix Composition in schizophrenia: A Pathophysiological Dysregulation and a Potential Therapeutic Target.
33712885	0	77	dep	Changes	0:6	arg1	Dysregulation					93:105	A Pathophysiological Dysregulation	72:105	A Pathophysiological Dysregulation	72:105	Changes in the Brain Extracellular Matrix Composition in schizophrenia: A Pathophysiological Dysregulation and a Potential Therapeutic Target.
33712885	1	78	theme	extracellular	153:165	arg1	ECM					175:177	ECM	175:177	ECM	175:177	The brain extracellular matrix (ECM) is involved in crucial processes of neural support, neuronal and synaptic plasticity, extrasynaptic transmission, and neurotransmission.
33712885	1	78	theme	extracellular	153:165	arg1	matrix					167:172	The brain extracellular matrix	143:172	The brain extracellular matrix (ECM)	143:178	The brain extracellular matrix (ECM) is involved in crucial processes of neural support, neuronal and synaptic plasticity, extrasynaptic transmission, and neurotransmission.
33712885	10	79	theme	neurodegenerative	1577:1593	arg1	processes					1595:1603	neurodegenerative processes	1577:1603	neurodegenerative processes due to lack of neurophysiological support and dysregulation of neuronal homeostasis	1577:1687	Our study proposes a pathophysiological dysregulation of proteins of the brain ECM, whose abnormal composition leads to a progressive neuronal impairment and consequently to neurodegenerative processes due to lack of neurophysiological support and dysregulation of neuronal homeostasis.
33712885	1	80	theme	neuronal	232:239	arg1	plasticity					254:263	neuronal and synaptic plasticity	232:263	plasticity	254:263	The brain extracellular matrix (ECM) is involved in crucial processes of neural support, neuronal and synaptic plasticity, extrasynaptic transmission, and neurotransmission.
34126977	4	0	dep	mulberry	447:454	arg1	leaves					456:461	leaves	456:461	leaves	456:461	Since mulberry leaves are able to improve the diabetic conditions through reducing blood glucose level, it would be interesting to investigate whether they have any positive effects on intestinal microbiota and liver glycogen structure.
34126977	12	1	theme	ethanol	1747:1753	arg1	extract					1755:1761	ethanol extract	1747:1761	ethanol extract of mulberry leaves	1747:1780	In addition, liver glycogen was restored from fragile state to stable state through administration of ethanol extract of mulberry leaves.
34126977	11	2	theme	Intestinal	1495:1504	arg1	analysis					1517:1524	Intestinal microbiota analysis	1495:1524	Intestinal microbiota analysis	1495:1524	Intestinal microbiota analysis showed that ethanol extract of mulberry leaves could partially change intestinal microbiota back to normal conditions.
34126977	6	3	theme	animal	894:899	arg1	models					901:906	Murine animal models	887:906	Murine animal models	887:906	Murine animal models were divided into three groups, normal Sprague-Dawley (SD) rats, high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats, and HFD/STZ-induced rats administered with ethanol extract of mulberry leaves (200 mg/kg/day).
34126977	6	4	dep	mulberry	1110:1117	arg1	leaves					1119:1124	leaves	1119:1124	leaves	1119:1124	Murine animal models were divided into three groups, normal Sprague-Dawley (SD) rats, high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats, and HFD/STZ-induced rats administered with ethanol extract of mulberry leaves (200 mg/kg/day).
34126977	5	5	dep	mulberry	768:775	arg1	leaves					777:782	leaves	777:782	leaves via high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS)	777:884	METHODS In this study, we first determined the bioactive components of ethanol extract of mulberry leaves via high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS).
34126977	13	6	theme	diabetic	1953:1960	arg1	rats					1962:1965	diabetic rats	1953:1965	diabetic rats	1953:1965	CONCLUSIONS This study confirms that the ethanol extract of mulberry leaves (MLE) ameliorates intestinal microbiota dysbiosis and strengthens liver glycogen fragility in diabetic rats.
34126977	14	7	dep	the	2047:2049	arg1	help					2051:2054	help	2051:2054	help	2051:2054	These finding can be helpful in discovering the novel therapeutic targets with the help of further investigations.
34126977	9	8	theme	statistical	1396:1406	arg1	analysis					1408:1415	statistical analysis	1396:1415	statistical analysis	1396:1415	Both Student's t-test and Tukey's test were used for statistical analysis.
34126977	6	9	theme	mulberry	1110:1117	arg1	extract					1099:1105	ethanol extract	1091:1105	ethanol extract of mulberry leaves (200 mg/kg/day)	1091:1140	Murine animal models were divided into three groups, normal Sprague-Dawley (SD) rats, high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats, and HFD/STZ-induced rats administered with ethanol extract of mulberry leaves (200 mg/kg/day).
34126977	1	10	theme	Chinese	203:209	arg1	medicine					211:218	a traditional Chinese medicine	189:218	a traditional Chinese medicine	189:218	BACKGROUND Mulberry leaf as a traditional Chinese medicine is able to treat obesity, diabetes, and dyslipidemia.
34126977	14	11	with	targets	2034:2040	arg1	the					2047:2049	the	2047:2049	the	2047:2049	These finding can be helpful in discovering the novel therapeutic targets with the help of further investigations.
34126977	5	12	theme	chromatography/mass	845:863	arg1	LC/MS					879:883	LC/MS	879:883	LC/MS	879:883	METHODS In this study, we first determined the bioactive components of ethanol extract of mulberry leaves via high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS).
34126977	5	12	theme	chromatography/mass	845:863	arg1	spectrometry					865:876	liquid chromatography/mass spectrometry	838:876	liquid chromatography/mass spectrometry (LC/MS)	838:884	METHODS In this study, we first determined the bioactive components of ethanol extract of mulberry leaves via high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS).
34126977	6	13	theme	normal	940:945	arg1	SD					963:964	SD	963:964	SD	963:964	Murine animal models were divided into three groups, normal Sprague-Dawley (SD) rats, high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats, and HFD/STZ-induced rats administered with ethanol extract of mulberry leaves (200 mg/kg/day).
34126977	6	13	theme	normal	940:945	arg1	Sprague-Dawley					947:960	normal Sprague-Dawley	940:960	normal Sprague-Dawley (SD) rats	940:970	Murine animal models were divided into three groups, normal Sprague-Dawley (SD) rats, high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats, and HFD/STZ-induced rats administered with ethanol extract of mulberry leaves (200 mg/kg/day).
34126977	8	14	theme	glycogen	1261:1268	arg1	structure					1270:1278	Liver glycogen structure	1255:1278	Liver glycogen structure	1255:1278	Liver glycogen structure was characterized through size exclusion chromatography (SEC).
34126977	0	15	theme	diabetic	146:153	arg1	rats					155:158	type 2 diabetic rats	139:158	type 2 diabetic rats	139:158	Ethanol extract of mulberry leaves partially restores the composition of intestinal microbiota and strengthens liver glycogen fragility in type 2 diabetic rats.
34126977	12	16	theme	stable	1708:1713	arg1	state					1715:1719	stable state	1708:1719	stable state through administration of ethanol extract of mulberry leaves	1708:1780	In addition, liver glycogen was restored from fragile state to stable state through administration of ethanol extract of mulberry leaves.
34126977	13	17	theme	glycogen	1931:1938	arg1	fragility					1940:1948	liver glycogen fragility	1925:1948	liver glycogen fragility in diabetic rats	1925:1965	CONCLUSIONS This study confirms that the ethanol extract of mulberry leaves (MLE) ameliorates intestinal microbiota dysbiosis and strengthens liver glycogen fragility in diabetic rats.
34126977	5	18	theme	ethanol	749:755	arg1	extract					757:763	ethanol extract	749:763	ethanol extract of mulberry leaves via high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS)	749:884	METHODS In this study, we first determined the bioactive components of ethanol extract of mulberry leaves via high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS).
34126977	4	19	contain	have	597:600	arg2	effects					615:621	any positive effects	602:621	any positive effects on intestinal microbiota and liver glycogen structure	602:675	Since mulberry leaves are able to improve the diabetic conditions through reducing blood glucose level, it would be interesting to investigate whether they have any positive effects on intestinal microbiota and liver glycogen structure.
34126977	4	19	contain	have	597:600	arg1	they					592:595	they	592:595	they	592:595	Since mulberry leaves are able to improve the diabetic conditions through reducing blood glucose level, it would be interesting to investigate whether they have any positive effects on intestinal microbiota and liver glycogen structure.
34126977	8	20	theme	exclusion	1311:1319	arg1	SEC					1337:1339	SEC	1337:1339	SEC	1337:1339	Liver glycogen structure was characterized through size exclusion chromatography (SEC).
34126977	8	20	theme	exclusion	1311:1319	arg1	chromatography					1321:1334	size exclusion chromatography	1306:1334	size exclusion chromatography (SEC)	1306:1340	Liver glycogen structure was characterized through size exclusion chromatography (SEC).
34126977	13	21	theme	intestinal	1877:1886	arg1	dysbiosis					1899:1907	intestinal microbiota dysbiosis	1877:1907	intestinal microbiota dysbiosis	1877:1907	CONCLUSIONS This study confirms that the ethanol extract of mulberry leaves (MLE) ameliorates intestinal microbiota dysbiosis and strengthens liver glycogen fragility in diabetic rats.
34126977	0	22	theme	intestinal	73:82	arg1	microbiota					84:93	intestinal microbiota	73:93	intestinal microbiota	73:93	Ethanol extract of mulberry leaves partially restores the composition of intestinal microbiota and strengthens liver glycogen fragility in type 2 diabetic rats.
34126977	1	23	theme	BACKGROUND	161:170	arg1	leaf					181:184	BACKGROUND Mulberry leaf	161:184	BACKGROUND Mulberry leaf as a traditional Chinese medicine	161:218	BACKGROUND Mulberry leaf as a traditional Chinese medicine is able to treat obesity, diabetes, and dyslipidemia.
34126977	6	24	theme	diet	982:985	arg1	groups					932:937	three groups	926:937	three groups	926:937	Murine animal models were divided into three groups, normal Sprague-Dawley (SD) rats, high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats, and HFD/STZ-induced rats administered with ethanol extract of mulberry leaves (200 mg/kg/day).
34126977	6	24	theme	diet	982:985	arg1	rats					1042:1045	high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats	973:1045	high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats	973:1045	Murine animal models were divided into three groups, normal Sprague-Dawley (SD) rats, high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats, and HFD/STZ-induced rats administered with ethanol extract of mulberry leaves (200 mg/kg/day).
34126977	14	25	theme	therapeutic	2022:2032	arg1	targets					2034:2040	the novel therapeutic targets	2012:2040	the novel therapeutic targets with the help of further investigations	2012:2080	These finding can be helpful in discovering the novel therapeutic targets with the help of further investigations.
34126977	3	26	theme	diabetic	423:430	arg1	animals					432:438	diabetic animals	423:438	diabetic animals	423:438	It is also recently discovered that liver glycogen structure is impaired in diabetic animals.
34126977	7	27	theme	intestinal	1158:1167	arg1	microbiota					1169:1178	intestinal microbiota	1158:1178	intestinal microbiota	1158:1178	Composition of intestinal microbiota was analyzed via metagenomics by sequencing the V3-V4 region of 16S rDNAs.
34126977	12	28	theme	liver	1658:1662	arg1	glycogen					1664:1671	liver glycogen	1658:1671	liver glycogen	1658:1671	In addition, liver glycogen was restored from fragile state to stable state through administration of ethanol extract of mulberry leaves.
34126977	6	29	theme	diabetic	1033:1040	arg1	groups					932:937	three groups	926:937	three groups	926:937	Murine animal models were divided into three groups, normal Sprague-Dawley (SD) rats, high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats, and HFD/STZ-induced rats administered with ethanol extract of mulberry leaves (200 mg/kg/day).
34126977	6	29	theme	diabetic	1033:1040	arg1	rats					1042:1045	high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats	973:1045	high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats	973:1045	Murine animal models were divided into three groups, normal Sprague-Dawley (SD) rats, high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats, and HFD/STZ-induced rats administered with ethanol extract of mulberry leaves (200 mg/kg/day).
34126977	0	30	theme	Ethanol	0:6	arg1	extract					8:14	Ethanol extract	0:14	Ethanol extract of mulberry	0:26	Ethanol extract of mulberry leaves partially restores the composition of intestinal microbiota and strengthens liver glycogen fragility in type 2 diabetic rats.
34126977	6	31	theme	type	1026:1029	arg1	groups					932:937	three groups	926:937	three groups	926:937	Murine animal models were divided into three groups, normal Sprague-Dawley (SD) rats, high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats, and HFD/STZ-induced rats administered with ethanol extract of mulberry leaves (200 mg/kg/day).
34126977	6	31	theme	type	1026:1029	arg1	rats					1042:1045	high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats	973:1045	high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats	973:1045	Murine animal models were divided into three groups, normal Sprague-Dawley (SD) rats, high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats, and HFD/STZ-induced rats administered with ethanol extract of mulberry leaves (200 mg/kg/day).
34126977	10	32	theme	models	1457:1462	arg1	group					1428:1432	A group	1426:1432	RESULTS A group of type 2 diabetic rat models	1418:1462	RESULTS A group of type 2 diabetic rat models were successfully established.
34126977	10	32	theme	models	1457:1462	arg1	models					1457:1462	type 2 diabetic rat models	1437:1462	type 2 diabetic rat models	1437:1462	RESULTS A group of type 2 diabetic rat models were successfully established.
34126977	10	33	theme	diabetic	1444:1451	arg1	models					1457:1462	type 2 diabetic rat models	1437:1462	type 2 diabetic rat models	1437:1462	RESULTS A group of type 2 diabetic rat models were successfully established.
34126977	13	34	theme	mulberry	1843:1850	arg1	extract					1832:1838	the ethanol extract	1820:1838	the ethanol extract of mulberry leaves (MLE)	1820:1863	CONCLUSIONS This study confirms that the ethanol extract of mulberry leaves (MLE) ameliorates intestinal microbiota dysbiosis and strengthens liver glycogen fragility in diabetic rats.
34126977	4	35	theme	liver	652:656	arg1	structure					667:675	liver glycogen structure	652:675	liver glycogen structure	652:675	Since mulberry leaves are able to improve the diabetic conditions through reducing blood glucose level, it would be interesting to investigate whether they have any positive effects on intestinal microbiota and liver glycogen structure.
34126977	3	36	theme	liver	383:387	arg1	glycogen					389:396	liver glycogen	383:396	liver glycogen structure	383:406	It is also recently discovered that liver glycogen structure is impaired in diabetic animals.
34126977	2	37	theme	microbiota	325:334	arg1	dysbiosis					336:344	intestinal microbiota dysbiosis	314:344	intestinal microbiota dysbiosis	314:344	It is well known that diabetes leads to intestinal microbiota dysbiosis.
34126977	5	38	theme	high-performance	788:803	arg1	chromatography					812:825	high-performance liquid chromatography	788:825	high-performance liquid chromatography (HPLC)	788:832	METHODS In this study, we first determined the bioactive components of ethanol extract of mulberry leaves via high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS).
34126977	5	38	theme	high-performance	788:803	arg1	HPLC					828:831	HPLC	828:831	HPLC	828:831	METHODS In this study, we first determined the bioactive components of ethanol extract of mulberry leaves via high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS).
34126977	0	39	dep	leaves	28:33	arg1	strengthens					99:109	strengthens	99:109	strengthens liver glycogen fragility in type 2 diabetic rats	99:158	Ethanol extract of mulberry leaves partially restores the composition of intestinal microbiota and strengthens liver glycogen fragility in type 2 diabetic rats.
34126977	0	39	dep	leaves	28:33	arg1	restores					45:52	restores	45:52	partially restores the composition of intestinal microbiota	35:93	Ethanol extract of mulberry leaves partially restores the composition of intestinal microbiota and strengthens liver glycogen fragility in type 2 diabetic rats.
34126977	6	40	theme	ethanol	1091:1097	arg1	extract					1099:1105	ethanol extract	1091:1105	ethanol extract of mulberry leaves (200 mg/kg/day)	1091:1140	Murine animal models were divided into three groups, normal Sprague-Dawley (SD) rats, high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats, and HFD/STZ-induced rats administered with ethanol extract of mulberry leaves (200 mg/kg/day).
34126977	4	41	theme	glucose	530:536	arg1	level					538:542	blood glucose level	524:542	blood glucose level	524:542	Since mulberry leaves are able to improve the diabetic conditions through reducing blood glucose level, it would be interesting to investigate whether they have any positive effects on intestinal microbiota and liver glycogen structure.
34126977	7	42	theme	16S	1244:1246	arg1	rDNAs					1248:1252	16S rDNAs	1244:1252	16S rDNAs	1244:1252	Composition of intestinal microbiota was analyzed via metagenomics by sequencing the V3-V4 region of 16S rDNAs.
34126977	9	43	used	used	1387:1390	arg2	t-test					1358:1363	Student's t-test	1348:1363	Student's t-test	1348:1363	Both Student's t-test and Tukey's test were used for statistical analysis.
34126977	9	43	used	used	1387:1390	arg2	test					1377:1380	Tukey's test	1369:1380	Tukey's test	1369:1380	Both Student's t-test and Tukey's test were used for statistical analysis.
34126977	6	44	theme	HFD/STZ-induced	1052:1066	arg1	groups					932:937	three groups	926:937	three groups	926:937	Murine animal models were divided into three groups, normal Sprague-Dawley (SD) rats, high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats, and HFD/STZ-induced rats administered with ethanol extract of mulberry leaves (200 mg/kg/day).
34126977	6	44	theme	HFD/STZ-induced	1052:1066	arg1	rats					1068:1071	HFD/STZ-induced rats	1052:1071	HFD/STZ-induced rats	1052:1071	Murine animal models were divided into three groups, normal Sprague-Dawley (SD) rats, high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats, and HFD/STZ-induced rats administered with ethanol extract of mulberry leaves (200 mg/kg/day).
34126977	0	45	from	fragility	126:134	arg1	rats					155:158	type 2 diabetic rats	139:158	type 2 diabetic rats	139:158	Ethanol extract of mulberry leaves partially restores the composition of intestinal microbiota and strengthens liver glycogen fragility in type 2 diabetic rats.
34126977	5	46	theme	extract	757:763	arg1	components					735:744	the bioactive components	721:744	the bioactive components of ethanol extract of mulberry leaves via high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS)	721:884	METHODS In this study, we first determined the bioactive components of ethanol extract of mulberry leaves via high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS).
34126977	11	47	theme	intestinal	1596:1605	arg1	microbiota					1607:1616	intestinal microbiota	1596:1616	intestinal microbiota	1596:1616	Intestinal microbiota analysis showed that ethanol extract of mulberry leaves could partially change intestinal microbiota back to normal conditions.
34126977	12	48	theme	extract	1755:1761	arg1	administration					1729:1742	administration	1729:1742	administration of ethanol extract of mulberry leaves	1729:1780	In addition, liver glycogen was restored from fragile state to stable state through administration of ethanol extract of mulberry leaves.
34126977	11	49	theme	normal	1626:1631	arg1	conditions					1633:1642	normal conditions	1626:1642	normal conditions	1626:1642	Intestinal microbiota analysis showed that ethanol extract of mulberry leaves could partially change intestinal microbiota back to normal conditions.
34126977	12	50	theme	mulberry	1766:1773	arg1	extract					1755:1761	ethanol extract	1747:1761	ethanol extract of mulberry leaves	1747:1780	In addition, liver glycogen was restored from fragile state to stable state through administration of ethanol extract of mulberry leaves.
34126977	11	51	dep	mulberry	1557:1564	arg1	leaves					1566:1571	leaves	1566:1571	leaves	1566:1571	Intestinal microbiota analysis showed that ethanol extract of mulberry leaves could partially change intestinal microbiota back to normal conditions.
34126977	11	52	theme	microbiota	1506:1515	arg1	analysis					1517:1524	Intestinal microbiota analysis	1495:1524	Intestinal microbiota analysis	1495:1524	Intestinal microbiota analysis showed that ethanol extract of mulberry leaves could partially change intestinal microbiota back to normal conditions.
34126977	1	53	theme	Mulberry	172:179	arg1	leaf					181:184	BACKGROUND Mulberry leaf	161:184	BACKGROUND Mulberry leaf as a traditional Chinese medicine	161:218	BACKGROUND Mulberry leaf as a traditional Chinese medicine is able to treat obesity, diabetes, and dyslipidemia.
34126977	6	54	theme	Murine	887:892	arg1	models					901:906	Murine animal models	887:906	Murine animal models	887:906	Murine animal models were divided into three groups, normal Sprague-Dawley (SD) rats, high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats, and HFD/STZ-induced rats administered with ethanol extract of mulberry leaves (200 mg/kg/day).
34126977	4	55	theme	glycogen	658:665	arg1	structure					667:675	liver glycogen structure	652:675	liver glycogen structure	652:675	Since mulberry leaves are able to improve the diabetic conditions through reducing blood glucose level, it would be interesting to investigate whether they have any positive effects on intestinal microbiota and liver glycogen structure.
34126977	11	56	theme	ethanol	1538:1544	arg1	extract					1546:1552	ethanol extract	1538:1552	ethanol extract of mulberry leaves	1538:1571	Intestinal microbiota analysis showed that ethanol extract of mulberry leaves could partially change intestinal microbiota back to normal conditions.
34126977	1	57	theme	traditional	191:201	arg1	medicine					211:218	a traditional Chinese medicine	189:218	a traditional Chinese medicine	189:218	BACKGROUND Mulberry leaf as a traditional Chinese medicine is able to treat obesity, diabetes, and dyslipidemia.
34126977	4	58	from	effects	615:621	arg1	microbiota					637:646	intestinal microbiota	626:646	intestinal microbiota	626:646	Since mulberry leaves are able to improve the diabetic conditions through reducing blood glucose level, it would be interesting to investigate whether they have any positive effects on intestinal microbiota and liver glycogen structure.
34126977	4	58	from	effects	615:621	arg1	structure					667:675	liver glycogen structure	652:675	liver glycogen structure	652:675	Since mulberry leaves are able to improve the diabetic conditions through reducing blood glucose level, it would be interesting to investigate whether they have any positive effects on intestinal microbiota and liver glycogen structure.
34126977	5	59	theme	liquid	838:843	arg1	LC/MS					879:883	LC/MS	879:883	LC/MS	879:883	METHODS In this study, we first determined the bioactive components of ethanol extract of mulberry leaves via high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS).
34126977	5	59	theme	liquid	838:843	arg1	spectrometry					865:876	liquid chromatography/mass spectrometry	838:876	liquid chromatography/mass spectrometry (LC/MS)	838:884	METHODS In this study, we first determined the bioactive components of ethanol extract of mulberry leaves via high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS).
34126977	6	60	theme	Sprague-Dawley	947:960	arg1	groups					932:937	three groups	926:937	three groups	926:937	Murine animal models were divided into three groups, normal Sprague-Dawley (SD) rats, high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats, and HFD/STZ-induced rats administered with ethanol extract of mulberry leaves (200 mg/kg/day).
34126977	6	60	theme	Sprague-Dawley	947:960	arg1	rats					967:970	normal Sprague-Dawley (SD) rats	940:970	normal Sprague-Dawley (SD) rats	940:970	Murine animal models were divided into three groups, normal Sprague-Dawley (SD) rats, high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats, and HFD/STZ-induced rats administered with ethanol extract of mulberry leaves (200 mg/kg/day).
34126977	4	61	theme	intestinal	626:635	arg1	microbiota					637:646	intestinal microbiota	626:646	intestinal microbiota	626:646	Since mulberry leaves are able to improve the diabetic conditions through reducing blood glucose level, it would be interesting to investigate whether they have any positive effects on intestinal microbiota and liver glycogen structure.
34126977	8	62	theme	Liver	1255:1259	arg1	structure					1270:1278	Liver glycogen structure	1255:1278	Liver glycogen structure	1255:1278	Liver glycogen structure was characterized through size exclusion chromatography (SEC).
34126977	13	63	from	fragility	1940:1948	arg1	rats					1962:1965	diabetic rats	1953:1965	diabetic rats	1953:1965	CONCLUSIONS This study confirms that the ethanol extract of mulberry leaves (MLE) ameliorates intestinal microbiota dysbiosis and strengthens liver glycogen fragility in diabetic rats.
34126977	12	64	theme	fragile	1691:1697	arg1	state					1699:1703	fragile state	1691:1703	fragile state	1691:1703	In addition, liver glycogen was restored from fragile state to stable state through administration of ethanol extract of mulberry leaves.
34126977	6	65	theme	streptozotocin	997:1010	arg1	groups					932:937	three groups	926:937	three groups	926:937	Murine animal models were divided into three groups, normal Sprague-Dawley (SD) rats, high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats, and HFD/STZ-induced rats administered with ethanol extract of mulberry leaves (200 mg/kg/day).
34126977	6	65	theme	streptozotocin	997:1010	arg1	rats					1042:1045	high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats	973:1045	high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats	973:1045	Murine animal models were divided into three groups, normal Sprague-Dawley (SD) rats, high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats, and HFD/STZ-induced rats administered with ethanol extract of mulberry leaves (200 mg/kg/day).
34126977	13	66	theme	liver	1925:1929	arg1	fragility					1940:1948	liver glycogen fragility	1925:1948	liver glycogen fragility in diabetic rats	1925:1965	CONCLUSIONS This study confirms that the ethanol extract of mulberry leaves (MLE) ameliorates intestinal microbiota dysbiosis and strengthens liver glycogen fragility in diabetic rats.
34126977	4	67	theme	diabetic	487:494	arg1	conditions					496:505	the diabetic conditions	483:505	the diabetic conditions	483:505	Since mulberry leaves are able to improve the diabetic conditions through reducing blood glucose level, it would be interesting to investigate whether they have any positive effects on intestinal microbiota and liver glycogen structure.
34126977	12	68	dep	mulberry	1766:1773	arg1	leaves					1775:1780	leaves	1775:1780	leaves	1775:1780	In addition, liver glycogen was restored from fragile state to stable state through administration of ethanol extract of mulberry leaves.
34126977	0	69	theme	microbiota	84:93	arg1	composition					58:68	the composition	54:68	the composition of intestinal microbiota	54:93	Ethanol extract of mulberry leaves partially restores the composition of intestinal microbiota and strengthens liver glycogen fragility in type 2 diabetic rats.
34126977	8	70	theme	size	1306:1309	arg1	SEC					1337:1339	SEC	1337:1339	SEC	1337:1339	Liver glycogen structure was characterized through size exclusion chromatography (SEC).
34126977	8	70	theme	size	1306:1309	arg1	chromatography					1321:1334	size exclusion chromatography	1306:1334	size exclusion chromatography (SEC)	1306:1340	Liver glycogen structure was characterized through size exclusion chromatography (SEC).
34126977	13	71	theme	microbiota	1888:1897	arg1	dysbiosis					1899:1907	intestinal microbiota dysbiosis	1877:1907	intestinal microbiota dysbiosis	1877:1907	CONCLUSIONS This study confirms that the ethanol extract of mulberry leaves (MLE) ameliorates intestinal microbiota dysbiosis and strengthens liver glycogen fragility in diabetic rats.
34126977	7	72	theme	microbiota	1169:1178	arg1	Composition					1143:1153	Composition	1143:1153	Composition of intestinal microbiota	1143:1178	Composition of intestinal microbiota was analyzed via metagenomics by sequencing the V3-V4 region of 16S rDNAs.
34126977	6	73	theme	high-fat	973:980	arg1	diet					982:985	high-fat diet	973:985	high-fat diet (HFD)	973:991	Murine animal models were divided into three groups, normal Sprague-Dawley (SD) rats, high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats, and HFD/STZ-induced rats administered with ethanol extract of mulberry leaves (200 mg/kg/day).
34126977	6	73	theme	high-fat	973:980	arg1	HFD					988:990	HFD	988:990	HFD	988:990	Murine animal models were divided into three groups, normal Sprague-Dawley (SD) rats, high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats, and HFD/STZ-induced rats administered with ethanol extract of mulberry leaves (200 mg/kg/day).
34126977	0	74	theme	glycogen	117:124	arg1	fragility					126:134	liver glycogen fragility	111:134	liver glycogen fragility in type 2 diabetic rats	111:158	Ethanol extract of mulberry leaves partially restores the composition of intestinal microbiota and strengthens liver glycogen fragility in type 2 diabetic rats.
34126977	14	75	theme	novel	2016:2020	arg1	targets					2034:2040	the novel therapeutic targets	2012:2040	the novel therapeutic targets with the help of further investigations	2012:2080	These finding can be helpful in discovering the novel therapeutic targets with the help of further investigations.
34126977	5	76	dep	METHODS	678:684	arg1	determined					710:719	determined	710:719	determined the bioactive components of ethanol extract of mulberry leaves via high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS)	710:884	METHODS In this study, we first determined the bioactive components of ethanol extract of mulberry leaves via high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS).
34126977	5	77	theme	bioactive	725:733	arg1	components					735:744	the bioactive components	721:744	the bioactive components of ethanol extract of mulberry leaves via high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS)	721:884	METHODS In this study, we first determined the bioactive components of ethanol extract of mulberry leaves via high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS).
34126977	5	78	theme	liquid	805:810	arg1	chromatography					812:825	high-performance liquid chromatography	788:825	high-performance liquid chromatography (HPLC)	788:832	METHODS In this study, we first determined the bioactive components of ethanol extract of mulberry leaves via high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS).
34126977	5	78	theme	liquid	805:810	arg1	HPLC					828:831	HPLC	828:831	HPLC	828:831	METHODS In this study, we first determined the bioactive components of ethanol extract of mulberry leaves via high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS).
34126977	13	79	theme	ethanol	1824:1830	arg1	extract					1832:1838	the ethanol extract	1820:1838	the ethanol extract of mulberry leaves (MLE)	1820:1863	CONCLUSIONS This study confirms that the ethanol extract of mulberry leaves (MLE) ameliorates intestinal microbiota dysbiosis and strengthens liver glycogen fragility in diabetic rats.
34126977	0	80	theme	mulberry	19:26	arg1	extract					8:14	Ethanol extract	0:14	Ethanol extract of mulberry	0:26	Ethanol extract of mulberry leaves partially restores the composition of intestinal microbiota and strengthens liver glycogen fragility in type 2 diabetic rats.
34126977	6	81	theme	induced	1018:1024	arg1	groups					932:937	three groups	926:937	three groups	926:937	Murine animal models were divided into three groups, normal Sprague-Dawley (SD) rats, high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats, and HFD/STZ-induced rats administered with ethanol extract of mulberry leaves (200 mg/kg/day).
34126977	6	81	theme	induced	1018:1024	arg1	rats					1042:1045	high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats	973:1045	high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats	973:1045	Murine animal models were divided into three groups, normal Sprague-Dawley (SD) rats, high-fat diet (HFD) and streptozotocin (STZ) induced type 2 diabetic rats, and HFD/STZ-induced rats administered with ethanol extract of mulberry leaves (200 mg/kg/day).
34126977	10	82	theme	rat	1453:1455	arg1	models					1457:1462	type 2 diabetic rat models	1437:1462	type 2 diabetic rat models	1437:1462	RESULTS A group of type 2 diabetic rat models were successfully established.
34126977	5	83	theme	mulberry	768:775	arg1	extract					757:763	ethanol extract	749:763	ethanol extract of mulberry leaves via high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS)	749:884	METHODS In this study, we first determined the bioactive components of ethanol extract of mulberry leaves via high-performance liquid chromatography (HPLC) and liquid chromatography/mass spectrometry (LC/MS).
34126977	4	84	theme	blood	524:528	arg1	level					538:542	blood glucose level	524:542	blood glucose level	524:542	Since mulberry leaves are able to improve the diabetic conditions through reducing blood glucose level, it would be interesting to investigate whether they have any positive effects on intestinal microbiota and liver glycogen structure.
34126977	14	85	theme	further	2059:2065	arg1	investigations					2067:2080	further investigations	2059:2080	further investigations	2059:2080	These finding can be helpful in discovering the novel therapeutic targets with the help of further investigations.
34126977	0	86	theme	liver	111:115	arg1	fragility					126:134	liver glycogen fragility	111:134	liver glycogen fragility in type 2 diabetic rats	111:158	Ethanol extract of mulberry leaves partially restores the composition of intestinal microbiota and strengthens liver glycogen fragility in type 2 diabetic rats.
34126977	10	87	dep	RESULTS	1418:1424	arg1	group					1428:1432	A group	1426:1432	RESULTS A group of type 2 diabetic rat models	1418:1462	RESULTS A group of type 2 diabetic rat models were successfully established.
34126977	10	87	dep	RESULTS	1418:1424	arg1	models					1457:1462	type 2 diabetic rat models	1437:1462	type 2 diabetic rat models	1437:1462	RESULTS A group of type 2 diabetic rat models were successfully established.
34126977	13	88	dep	CONCLUSIONS	1783:1793	arg1	confirms					1806:1813	confirms	1806:1813	confirms that the ethanol extract of mulberry leaves (MLE) ameliorates intestinal microbiota dysbiosis and strengthens liver glycogen fragility in diabetic rats	1806:1965	CONCLUSIONS This study confirms that the ethanol extract of mulberry leaves (MLE) ameliorates intestinal microbiota dysbiosis and strengthens liver glycogen fragility in diabetic rats.
34126977	7	89	theme	rDNAs	1248:1252	arg1	region					1234:1239	the V3-V4 region	1224:1239	the V3-V4 region of 16S rDNAs	1224:1252	Composition of intestinal microbiota was analyzed via metagenomics by sequencing the V3-V4 region of 16S rDNAs.
34126977	3	90	theme	glycogen	389:396	arg1	structure					398:406	liver glycogen structure	383:406	liver glycogen structure	383:406	It is also recently discovered that liver glycogen structure is impaired in diabetic animals.
34126977	11	91	theme	mulberry	1557:1564	arg1	extract					1546:1552	ethanol extract	1538:1552	ethanol extract of mulberry leaves	1538:1571	Intestinal microbiota analysis showed that ethanol extract of mulberry leaves could partially change intestinal microbiota back to normal conditions.
34126977	2	92	theme	intestinal	314:323	arg1	dysbiosis					336:344	intestinal microbiota dysbiosis	314:344	intestinal microbiota dysbiosis	314:344	It is well known that diabetes leads to intestinal microbiota dysbiosis.
34126977	13	93	dep	mulberry	1843:1850	arg1	leaves					1852:1857	leaves	1852:1857	leaves	1852:1857	CONCLUSIONS This study confirms that the ethanol extract of mulberry leaves (MLE) ameliorates intestinal microbiota dysbiosis and strengthens liver glycogen fragility in diabetic rats.
34126977	7	94	theme	V3-V4	1228:1232	arg1	region					1234:1239	the V3-V4 region	1224:1239	the V3-V4 region of 16S rDNAs	1224:1252	Composition of intestinal microbiota was analyzed via metagenomics by sequencing the V3-V4 region of 16S rDNAs.
34126977	4	95	theme	positive	606:613	arg1	effects					615:621	any positive effects	602:621	any positive effects on intestinal microbiota and liver glycogen structure	602:675	Since mulberry leaves are able to improve the diabetic conditions through reducing blood glucose level, it would be interesting to investigate whether they have any positive effects on intestinal microbiota and liver glycogen structure.
32829821	0	0	theme	thin	72:75	arg1	film					77:80	chitosan/poly(vinyl alcohol)/ZIF-8 thin film	37:80	chitosan/poly(vinyl alcohol)/ZIF-8 thin film composite adsorptive membranes	37:111	Nanofiltration of dye solution using chitosan/poly(vinyl alcohol)/ZIF-8 thin film composite adsorptive membranes with PVDF membrane beneath as support.
32829821	1	1	theme	PVDF	324:327	arg1	support					338:344	PVDF membrane support	324:344	PVDF membrane support for an enhanced separation performance	324:383	Nanobiocomposite adsorptive membranes were engineered by integrating metal-organic frameworks (ZIF-8), into the chitosan/poly(vinyl alcohol) blend and coating the blend on PVDF membrane support for an enhanced separation performance.
32829821	5	2	theme	membrane	777:784	arg1	conditions					709:718	The optimal conditions	697:718	The optimal conditions	697:718	The optimal conditions were obtained as; 10 wt% PEG, 3 wt% chitosan, and 200 μm membrane thickness.
32829821	5	2	theme	membrane	777:784	arg1	thickness					786:794	200 μm membrane thickness	770:794	200 μm membrane thickness	770:794	The optimal conditions were obtained as; 10 wt% PEG, 3 wt% chitosan, and 200 μm membrane thickness.
32829821	0	3	theme	/ZIF-8	65:70	arg1	film					77:80	chitosan/poly(vinyl alcohol)/ZIF-8 thin film	37:80	chitosan/poly(vinyl alcohol)/ZIF-8 thin film composite adsorptive membranes	37:111	Nanofiltration of dye solution using chitosan/poly(vinyl alcohol)/ZIF-8 thin film composite adsorptive membranes with PVDF membrane beneath as support.
32829821	1	4	theme	membrane	329:336	arg1	support					338:344	PVDF membrane support	324:344	PVDF membrane support for an enhanced separation performance	324:383	Nanobiocomposite adsorptive membranes were engineered by integrating metal-organic frameworks (ZIF-8), into the chitosan/poly(vinyl alcohol) blend and coating the blend on PVDF membrane support for an enhanced separation performance.
32829821	1	5	from	blend	315:319	arg1	support					338:344	PVDF membrane support	324:344	PVDF membrane support for an enhanced separation performance	324:383	Nanobiocomposite adsorptive membranes were engineered by integrating metal-organic frameworks (ZIF-8), into the chitosan/poly(vinyl alcohol) blend and coating the blend on PVDF membrane support for an enhanced separation performance.
32829821	2	6	theme	tensile	455:461	arg1	stress					463:468	tensile stress	455:468	tensile stress	455:468	The membranes were characterized by SEM, AFM, BET, XRD, ATR-FTIR and tensile stress analyses.
32829821	1	7	theme	Nanobiocomposite	152:167	arg1	membranes					180:188	Nanobiocomposite adsorptive membranes	152:188	Nanobiocomposite adsorptive membranes	152:188	Nanobiocomposite adsorptive membranes were engineered by integrating metal-organic frameworks (ZIF-8), into the chitosan/poly(vinyl alcohol) blend and coating the blend on PVDF membrane support for an enhanced separation performance.
32829821	0	8	theme	composite	82:90	arg1	membranes					103:111	chitosan/poly(vinyl alcohol)/ZIF-8 thin film composite adsorptive membranes	37:111	chitosan/poly(vinyl alcohol)/ZIF-8 thin film composite adsorptive membranes	37:111	Nanofiltration of dye solution using chitosan/poly(vinyl alcohol)/ZIF-8 thin film composite adsorptive membranes with PVDF membrane beneath as support.
32829821	7	9	theme	solution	970:977	arg1	filtration					944:953	the filtration	940:953	the filtration of BSA protein solution	940:977	The antifouling of the membranes was also examined by the filtration of BSA protein solution.
32829821	5	10	theme	μm	774:775	arg1	conditions					709:718	The optimal conditions	697:718	The optimal conditions	697:718	The optimal conditions were obtained as; 10 wt% PEG, 3 wt% chitosan, and 200 μm membrane thickness.
32829821	5	10	theme	μm	774:775	arg1	thickness					786:794	200 μm membrane thickness	770:794	200 μm membrane thickness	770:794	The optimal conditions were obtained as; 10 wt% PEG, 3 wt% chitosan, and 200 μm membrane thickness.
32829821	1	11	theme	chitosan/poly	264:276	arg1	blend					293:297	the chitosan/poly(vinyl alcohol) blend	260:297	the chitosan/poly(vinyl alcohol) blend	260:297	Nanobiocomposite adsorptive membranes were engineered by integrating metal-organic frameworks (ZIF-8), into the chitosan/poly(vinyl alcohol) blend and coating the blend on PVDF membrane support for an enhanced separation performance.
32829821	0	12	theme	film	77:80	arg1	membranes					103:111	chitosan/poly(vinyl alcohol)/ZIF-8 thin film composite adsorptive membranes	37:111	chitosan/poly(vinyl alcohol)/ZIF-8 thin film composite adsorptive membranes	37:111	Nanofiltration of dye solution using chitosan/poly(vinyl alcohol)/ZIF-8 thin film composite adsorptive membranes with PVDF membrane beneath as support.
32829821	5	13	theme	optimal	701:707	arg1	chitosan					756:763	3 wt% chitosan	750:763	3 wt% chitosan	750:763	The optimal conditions were obtained as; 10 wt% PEG, 3 wt% chitosan, and 200 μm membrane thickness.
32829821	5	13	theme	optimal	701:707	arg1	conditions					709:718	The optimal conditions	697:718	The optimal conditions	697:718	The optimal conditions were obtained as; 10 wt% PEG, 3 wt% chitosan, and 200 μm membrane thickness.
32829821	5	13	theme	optimal	701:707	arg1	thickness					786:794	200 μm membrane thickness	770:794	200 μm membrane thickness	770:794	The optimal conditions were obtained as; 10 wt% PEG, 3 wt% chitosan, and 200 μm membrane thickness.
32829821	5	13	theme	optimal	701:707	arg1	PEG					745:747	10 wt% PEG	738:747	10 wt% PEG	738:747	The optimal conditions were obtained as; 10 wt% PEG, 3 wt% chitosan, and 200 μm membrane thickness.
32829821	8	14	theme	economic	1142:1149	arg1	favorability					1151:1162	the economic favorability	1138:1162	the economic favorability of the filtration process	1138:1188	The membranes showed comparable performance; however, extremely higher permeability (78.94 L m-2 h-1 bar -1) compared to similar TFC membranes that guarantee the economic favorability of the filtration process.
32829821	6	15	theme	optimized	801:809	arg1	flux					811:814	flux	811:814	flux	811:814	The optimized flux and rejection were obtained 78.94 L m-2 h-1 and 90.3 %, respectively.
32829821	8	16	theme	TFC	1109:1111	arg1	membranes					1113:1121	similar TFC membranes	1101:1121	similar TFC membranes that guarantee the economic favorability of the filtration process	1101:1188	The membranes showed comparable performance; however, extremely higher permeability (78.94 L m-2 h-1 bar -1) compared to similar TFC membranes that guarantee the economic favorability of the filtration process.
32829821	8	17	theme	78.94	1065:1069	arg1	permeability					1051:1062	extremely higher permeability	1034:1062	extremely higher permeability (78.94 L m-2 h-1 bar -1)	1034:1087	The membranes showed comparable performance; however, extremely higher permeability (78.94 L m-2 h-1 bar -1) compared to similar TFC membranes that guarantee the economic favorability of the filtration process.
32829821	8	17	theme	78.94	1065:1069	arg1	bar					1081:1083	78.94 L m-2 h-1 bar -1	1065:1086	78.94 L m-2 h-1 bar -1	1065:1086	The membranes showed comparable performance; however, extremely higher permeability (78.94 L m-2 h-1 bar -1) compared to similar TFC membranes that guarantee the economic favorability of the filtration process.
32829821	1	18	theme	adsorptive	169:178	arg1	membranes					180:188	Nanobiocomposite adsorptive membranes	152:188	Nanobiocomposite adsorptive membranes	152:188	Nanobiocomposite adsorptive membranes were engineered by integrating metal-organic frameworks (ZIF-8), into the chitosan/poly(vinyl alcohol) blend and coating the blend on PVDF membrane support for an enhanced separation performance.
32829821	0	19	theme	adsorptive	92:101	arg1	membranes					103:111	chitosan/poly(vinyl alcohol)/ZIF-8 thin film composite adsorptive membranes	37:111	chitosan/poly(vinyl alcohol)/ZIF-8 thin film composite adsorptive membranes	37:111	Nanofiltration of dye solution using chitosan/poly(vinyl alcohol)/ZIF-8 thin film composite adsorptive membranes with PVDF membrane beneath as support.
32829821	1	20	theme	enhanced	353:360	arg1	performance					373:383	an enhanced separation performance	350:383	an enhanced separation performance	350:383	Nanobiocomposite adsorptive membranes were engineered by integrating metal-organic frameworks (ZIF-8), into the chitosan/poly(vinyl alcohol) blend and coating the blend on PVDF membrane support for an enhanced separation performance.
32829821	2	21	theme	ATR-FTIR	442:449	arg1	analyses					470:477	SEM, AFM, BET, XRD, ATR-FTIR and tensile stress analyses	422:477	SEM, AFM, BET, XRD, ATR-FTIR and tensile stress analyses	422:477	The membranes were characterized by SEM, AFM, BET, XRD, ATR-FTIR and tensile stress analyses.
32829821	4	22	theme	thickness	606:614	arg1	effect					583:588	the effect	579:588	the effect of the membrane thickness, PEG (pore-former), and chitosan content on the flux and rejection	579:681	Using CCD, the effect of the membrane thickness, PEG (pore-former), and chitosan content on the flux and rejection were studied.
32829821	1	23	theme	separation	362:371	arg1	performance					373:383	an enhanced separation performance	350:383	an enhanced separation performance	350:383	Nanobiocomposite adsorptive membranes were engineered by integrating metal-organic frameworks (ZIF-8), into the chitosan/poly(vinyl alcohol) blend and coating the blend on PVDF membrane support for an enhanced separation performance.
32829821	3	24	theme	water	538:542	arg1	flux					544:547	water flux	538:547	water flux	538:547	The central composite design was utilized to optimize the water flux and dye rejection.
32829821	8	25	theme	similar	1101:1107	arg1	membranes					1113:1121	similar TFC membranes	1101:1121	similar TFC membranes that guarantee the economic favorability of the filtration process	1101:1188	The membranes showed comparable performance; however, extremely higher permeability (78.94 L m-2 h-1 bar -1) compared to similar TFC membranes that guarantee the economic favorability of the filtration process.
32829821	1	26	theme	vinyl	278:282	arg1	blend					293:297	the chitosan/poly(vinyl alcohol) blend	260:297	the chitosan/poly(vinyl alcohol) blend	260:297	Nanobiocomposite adsorptive membranes were engineered by integrating metal-organic frameworks (ZIF-8), into the chitosan/poly(vinyl alcohol) blend and coating the blend on PVDF membrane support for an enhanced separation performance.
32829821	7	27	theme	BSA	958:960	arg1	solution					970:977	BSA protein solution	958:977	BSA protein solution	958:977	The antifouling of the membranes was also examined by the filtration of BSA protein solution.
32829821	3	28	used	utilized	513:520	arg2	design					502:507	The central composite design	480:507	The central composite design	480:507	The central composite design was utilized to optimize the water flux and dye rejection.
32829821	0	29	theme	solution	22:29	arg1	Nanofiltration					0:13	Nanofiltration	0:13	Nanofiltration of dye solution using chitosan/poly(vinyl alcohol)/ZIF-8 thin film composite adsorptive membranes with PVDF membrane beneath as support.	0:150	Nanofiltration of dye solution using chitosan/poly(vinyl alcohol)/ZIF-8 thin film composite adsorptive membranes with PVDF membrane beneath as support.
32829821	1	30	theme	alcohol	284:290	arg1	blend					293:297	the chitosan/poly(vinyl alcohol) blend	260:297	the chitosan/poly(vinyl alcohol) blend	260:297	Nanobiocomposite adsorptive membranes were engineered by integrating metal-organic frameworks (ZIF-8), into the chitosan/poly(vinyl alcohol) blend and coating the blend on PVDF membrane support for an enhanced separation performance.
32829821	0	31	theme	PVDF	118:121	arg1	membrane					123:130	PVDF membrane beneath	118:138	PVDF membrane beneath as support	118:149	Nanofiltration of dye solution using chitosan/poly(vinyl alcohol)/ZIF-8 thin film composite adsorptive membranes with PVDF membrane beneath as support.
32829821	8	32	theme	L	1071:1071	arg1	permeability					1051:1062	extremely higher permeability	1034:1062	extremely higher permeability (78.94 L m-2 h-1 bar -1)	1034:1087	The membranes showed comparable performance; however, extremely higher permeability (78.94 L m-2 h-1 bar -1) compared to similar TFC membranes that guarantee the economic favorability of the filtration process.
32829821	8	32	theme	L	1071:1071	arg1	bar					1081:1083	78.94 L m-2 h-1 bar -1	1065:1086	78.94 L m-2 h-1 bar -1	1065:1086	The membranes showed comparable performance; however, extremely higher permeability (78.94 L m-2 h-1 bar -1) compared to similar TFC membranes that guarantee the economic favorability of the filtration process.
32829821	8	33	theme	h-1	1077:1079	arg1	permeability					1051:1062	extremely higher permeability	1034:1062	extremely higher permeability (78.94 L m-2 h-1 bar -1)	1034:1087	The membranes showed comparable performance; however, extremely higher permeability (78.94 L m-2 h-1 bar -1) compared to similar TFC membranes that guarantee the economic favorability of the filtration process.
32829821	8	33	theme	h-1	1077:1079	arg1	bar					1081:1083	78.94 L m-2 h-1 bar -1	1065:1086	78.94 L m-2 h-1 bar -1	1065:1086	The membranes showed comparable performance; however, extremely higher permeability (78.94 L m-2 h-1 bar -1) compared to similar TFC membranes that guarantee the economic favorability of the filtration process.
32829821	7	34	theme	protein	962:968	arg1	solution					970:977	BSA protein solution	958:977	BSA protein solution	958:977	The antifouling of the membranes was also examined by the filtration of BSA protein solution.
32829821	2	35	theme	SEM	422:424	arg1	analyses					470:477	SEM, AFM, BET, XRD, ATR-FTIR and tensile stress analyses	422:477	SEM, AFM, BET, XRD, ATR-FTIR and tensile stress analyses	422:477	The membranes were characterized by SEM, AFM, BET, XRD, ATR-FTIR and tensile stress analyses.
32829821	0	36	theme	dye	18:20	arg1	solution					22:29	dye solution	18:29	dye solution	18:29	Nanofiltration of dye solution using chitosan/poly(vinyl alcohol)/ZIF-8 thin film composite adsorptive membranes with PVDF membrane beneath as support.
32829821	6	37	theme	L	850:850	arg1	h-1					856:858	78.94 L m-2 h-1	844:858	78.94 L m-2 h-1	844:858	The optimized flux and rejection were obtained 78.94 L m-2 h-1 and 90.3 %, respectively.
32829821	4	38	from	effect	583:588	arg1	rejection					673:681	rejection	673:681	rejection	673:681	Using CCD, the effect of the membrane thickness, PEG (pore-former), and chitosan content on the flux and rejection were studied.
32829821	4	38	from	effect	583:588	arg1	flux					664:667	flux	664:667	flux	664:667	Using CCD, the effect of the membrane thickness, PEG (pore-former), and chitosan content on the flux and rejection were studied.
32829821	2	39	theme	XRD	437:439	arg1	analyses					470:477	SEM, AFM, BET, XRD, ATR-FTIR and tensile stress analyses	422:477	SEM, AFM, BET, XRD, ATR-FTIR and tensile stress analyses	422:477	The membranes were characterized by SEM, AFM, BET, XRD, ATR-FTIR and tensile stress analyses.
32829821	5	40	theme	200	770:772	arg1	μm					774:775	μm	774:775	μm	774:775	The optimal conditions were obtained as; 10 wt% PEG, 3 wt% chitosan, and 200 μm membrane thickness.
32829821	0	41	theme	chitosan/poly	37:49	arg1	film					77:80	chitosan/poly(vinyl alcohol)/ZIF-8 thin film	37:80	chitosan/poly(vinyl alcohol)/ZIF-8 thin film composite adsorptive membranes	37:111	Nanofiltration of dye solution using chitosan/poly(vinyl alcohol)/ZIF-8 thin film composite adsorptive membranes with PVDF membrane beneath as support.
32829821	6	42	theme	78.94	844:848	arg1	L					850:850	L	850:850	L	850:850	The optimized flux and rejection were obtained 78.94 L m-2 h-1 and 90.3 %, respectively.
32829821	4	43	theme	membrane	597:604	arg1	thickness					606:614	the membrane thickness	593:614	the membrane thickness	593:614	Using CCD, the effect of the membrane thickness, PEG (pore-former), and chitosan content on the flux and rejection were studied.
32829821	4	44	theme	PEG	617:619	arg1	effect					583:588	the effect	579:588	the effect of the membrane thickness, PEG (pore-former), and chitosan content on the flux and rejection	579:681	Using CCD, the effect of the membrane thickness, PEG (pore-former), and chitosan content on the flux and rejection were studied.
32829821	1	45	theme	metal-organic	221:233	arg1	ZIF-8					247:251	ZIF-8	247:251	ZIF-8	247:251	Nanobiocomposite adsorptive membranes were engineered by integrating metal-organic frameworks (ZIF-8), into the chitosan/poly(vinyl alcohol) blend and coating the blend on PVDF membrane support for an enhanced separation performance.
32829821	1	45	theme	metal-organic	221:233	arg1	frameworks					235:244	metal-organic frameworks	221:244	metal-organic frameworks (ZIF-8)	221:252	Nanobiocomposite adsorptive membranes were engineered by integrating metal-organic frameworks (ZIF-8), into the chitosan/poly(vinyl alcohol) blend and coating the blend on PVDF membrane support for an enhanced separation performance.
32829821	5	46	theme	%	754:754	arg1	chitosan					756:763	3 wt% chitosan	750:763	3 wt% chitosan	750:763	The optimal conditions were obtained as; 10 wt% PEG, 3 wt% chitosan, and 200 μm membrane thickness.
32829821	5	46	theme	%	754:754	arg1	conditions					709:718	The optimal conditions	697:718	The optimal conditions	697:718	The optimal conditions were obtained as; 10 wt% PEG, 3 wt% chitosan, and 200 μm membrane thickness.
32829821	8	47	theme	m-2	1073:1075	arg1	permeability					1051:1062	extremely higher permeability	1034:1062	extremely higher permeability (78.94 L m-2 h-1 bar -1)	1034:1087	The membranes showed comparable performance; however, extremely higher permeability (78.94 L m-2 h-1 bar -1) compared to similar TFC membranes that guarantee the economic favorability of the filtration process.
32829821	8	47	theme	m-2	1073:1075	arg1	bar					1081:1083	78.94 L m-2 h-1 bar -1	1065:1086	78.94 L m-2 h-1 bar -1	1065:1086	The membranes showed comparable performance; however, extremely higher permeability (78.94 L m-2 h-1 bar -1) compared to similar TFC membranes that guarantee the economic favorability of the filtration process.
32829821	2	48	theme	AFM	427:429	arg1	analyses					470:477	SEM, AFM, BET, XRD, ATR-FTIR and tensile stress analyses	422:477	SEM, AFM, BET, XRD, ATR-FTIR and tensile stress analyses	422:477	The membranes were characterized by SEM, AFM, BET, XRD, ATR-FTIR and tensile stress analyses.
32829821	5	49	theme	wt	741:742	arg1	PEG					745:747	10 wt% PEG	738:747	10 wt% PEG	738:747	The optimal conditions were obtained as; 10 wt% PEG, 3 wt% chitosan, and 200 μm membrane thickness.
32829821	5	49	theme	wt	741:742	arg1	conditions					709:718	The optimal conditions	697:718	The optimal conditions	697:718	The optimal conditions were obtained as; 10 wt% PEG, 3 wt% chitosan, and 200 μm membrane thickness.
32829821	0	50	theme	vinyl	51:55	arg1	film					77:80	chitosan/poly(vinyl alcohol)/ZIF-8 thin film	37:80	chitosan/poly(vinyl alcohol)/ZIF-8 thin film composite adsorptive membranes	37:111	Nanofiltration of dye solution using chitosan/poly(vinyl alcohol)/ZIF-8 thin film composite adsorptive membranes with PVDF membrane beneath as support.
32829821	7	51	theme	membranes	909:917	arg1	antifouling					890:900	The antifouling	886:900	The antifouling of the membranes	886:917	The antifouling of the membranes was also examined by the filtration of BSA protein solution.
32829821	2	52	theme	stress	463:468	arg1	analyses					470:477	SEM, AFM, BET, XRD, ATR-FTIR and tensile stress analyses	422:477	SEM, AFM, BET, XRD, ATR-FTIR and tensile stress analyses	422:477	The membranes were characterized by SEM, AFM, BET, XRD, ATR-FTIR and tensile stress analyses.
32829821	4	53	theme	content	649:655	arg1	effect					583:588	the effect	579:588	the effect of the membrane thickness, PEG (pore-former), and chitosan content on the flux and rejection	579:681	Using CCD, the effect of the membrane thickness, PEG (pore-former), and chitosan content on the flux and rejection were studied.
32829821	8	54	theme	process	1182:1188	arg1	favorability					1151:1162	the economic favorability	1138:1162	the economic favorability of the filtration process	1138:1188	The membranes showed comparable performance; however, extremely higher permeability (78.94 L m-2 h-1 bar -1) compared to similar TFC membranes that guarantee the economic favorability of the filtration process.
32829821	3	55	theme	dye	553:555	arg1	rejection					557:565	dye rejection	553:565	dye rejection	553:565	The central composite design was utilized to optimize the water flux and dye rejection.
32829821	4	56	theme	chitosan	640:647	arg1	content					649:655	chitosan content	640:655	chitosan content	640:655	Using CCD, the effect of the membrane thickness, PEG (pore-former), and chitosan content on the flux and rejection were studied.
32829821	8	57	theme	filtration	1171:1180	arg1	process					1182:1188	the filtration process	1167:1188	the filtration process	1167:1188	The membranes showed comparable performance; however, extremely higher permeability (78.94 L m-2 h-1 bar -1) compared to similar TFC membranes that guarantee the economic favorability of the filtration process.
32829821	4	58	dep	flux	664:667	arg1	the					660:662	the	660:662	the	660:662	Using CCD, the effect of the membrane thickness, PEG (pore-former), and chitosan content on the flux and rejection were studied.
32829821	6	59	theme	m-2	852:854	arg1	h-1					856:858	78.94 L m-2 h-1	844:858	78.94 L m-2 h-1	844:858	The optimized flux and rejection were obtained 78.94 L m-2 h-1 and 90.3 %, respectively.
32829821	8	60	theme	comparable	1001:1010	arg1	performance					1012:1022	comparable performance	1001:1022	comparable performance	1001:1022	The membranes showed comparable performance; however, extremely higher permeability (78.94 L m-2 h-1 bar -1) compared to similar TFC membranes that guarantee the economic favorability of the filtration process.
32829821	5	61	theme	wt	752:753	arg1	chitosan					756:763	3 wt% chitosan	750:763	3 wt% chitosan	750:763	The optimal conditions were obtained as; 10 wt% PEG, 3 wt% chitosan, and 200 μm membrane thickness.
32829821	5	61	theme	wt	752:753	arg1	conditions					709:718	The optimal conditions	697:718	The optimal conditions	697:718	The optimal conditions were obtained as; 10 wt% PEG, 3 wt% chitosan, and 200 μm membrane thickness.
32829821	8	62	theme	higher	1044:1049	arg1	permeability					1051:1062	extremely higher permeability	1034:1062	extremely higher permeability (78.94 L m-2 h-1 bar -1)	1034:1087	The membranes showed comparable performance; however, extremely higher permeability (78.94 L m-2 h-1 bar -1) compared to similar TFC membranes that guarantee the economic favorability of the filtration process.
32829821	8	62	theme	higher	1044:1049	arg1	bar					1081:1083	78.94 L m-2 h-1 bar -1	1065:1086	78.94 L m-2 h-1 bar -1	1065:1086	The membranes showed comparable performance; however, extremely higher permeability (78.94 L m-2 h-1 bar -1) compared to similar TFC membranes that guarantee the economic favorability of the filtration process.
32829821	3	63	theme	central	484:490	arg1	design					502:507	The central composite design	480:507	The central composite design	480:507	The central composite design was utilized to optimize the water flux and dye rejection.
32829821	0	64	theme	alcohol	57:63	arg1	film					77:80	chitosan/poly(vinyl alcohol)/ZIF-8 thin film	37:80	chitosan/poly(vinyl alcohol)/ZIF-8 thin film composite adsorptive membranes	37:111	Nanofiltration of dye solution using chitosan/poly(vinyl alcohol)/ZIF-8 thin film composite adsorptive membranes with PVDF membrane beneath as support.
32829821	5	65	theme	%	743:743	arg1	PEG					745:747	10 wt% PEG	738:747	10 wt% PEG	738:747	The optimal conditions were obtained as; 10 wt% PEG, 3 wt% chitosan, and 200 μm membrane thickness.
32829821	5	65	theme	%	743:743	arg1	conditions					709:718	The optimal conditions	697:718	The optimal conditions	697:718	The optimal conditions were obtained as; 10 wt% PEG, 3 wt% chitosan, and 200 μm membrane thickness.
32829821	3	66	theme	composite	492:500	arg1	design					502:507	The central composite design	480:507	The central composite design	480:507	The central composite design was utilized to optimize the water flux and dye rejection.
31931062	4	0	with	monomer	671:677	arg1	activity					701:708	no agglutination activity	684:708	no agglutination activity	684:708	Interestingly, under non-denaturing conditions, recombinant SHL1 (rSHL1) existed as a trimer and exhibited agglutination activity, whereas rHEL1 existed as a monomer with no agglutination activity.
31931062	1	1	theme	mushroom	149:156	arg1	erinaceus					167:175	The mushroom Hericium erinaceus	145:175	The mushroom Hericium erinaceus	145:175	The mushroom Hericium erinaceus contains isolectins, including the ricin B-like lectin HEL1 and the core 1 O-glycan-binding lectin HEL2.
31931062	1	2	theme	core	245:248	arg1	HEL2					276:279	the core 1 O-glycan-binding lectin HEL2	241:279	the core 1 O-glycan-binding lectin HEL2	241:279	The mushroom Hericium erinaceus contains isolectins, including the ricin B-like lectin HEL1 and the core 1 O-glycan-binding lectin HEL2.
31931062	6	3	theme	1,4	977:979	arg1	N-acetylglucosamine					982:1000	fucose-α(1,4) N-acetylglucosamine	968:1000	fucose-α(1,4) N-acetylglucosamine	968:1000	A glycan-array analysis revealed that the two recombinant lectins had different binding intensities toward fucosylated N-glycans harboring fucose-α(1,2) galactose or fucose-α(1,4) N-acetylglucosamine.
31931062	1	4	theme	Hericium	158:165	arg1	erinaceus					167:175	The mushroom Hericium erinaceus	145:175	The mushroom Hericium erinaceus	145:175	The mushroom Hericium erinaceus contains isolectins, including the ricin B-like lectin HEL1 and the core 1 O-glycan-binding lectin HEL2.
31931062	8	5	gly	fucosylated	1269:1279	arg1	N-glycans					1281:1289	highly fucosylated N-glycans	1262:1289	highly fucosylated N-glycans within glycoproteins on the surface of SW1116 human colorectal carcinoma cells	1262:1368	Finally, rSHL1 and rHEL1 were comparable in their ability to detect highly fucosylated N-glycans within glycoproteins on the surface of SW1116 human colorectal carcinoma cells.
31931062	4	6	theme	non-denaturing	534:547	arg1	conditions					549:558	non-denaturing conditions	534:558	non-denaturing conditions	534:558	Interestingly, under non-denaturing conditions, recombinant SHL1 (rSHL1) existed as a trimer and exhibited agglutination activity, whereas rHEL1 existed as a monomer with no agglutination activity.
31931062	1	7	theme	O-glycan-binding	252:267	arg1	HEL2					276:279	the core 1 O-glycan-binding lectin HEL2	241:279	the core 1 O-glycan-binding lectin HEL2	241:279	The mushroom Hericium erinaceus contains isolectins, including the ricin B-like lectin HEL1 and the core 1 O-glycan-binding lectin HEL2.
31931062	6	8	theme	binding	882:888	arg1	intensities					890:900	different binding intensities	872:900	different binding intensities	872:900	A glycan-array analysis revealed that the two recombinant lectins had different binding intensities toward fucosylated N-glycans harboring fucose-α(1,2) galactose or fucose-α(1,4) N-acetylglucosamine.
31931062	7	9	theme	fucosylated	1110:1120	arg1	transferrin					1095:1105	transferrin	1095:1105	transferrin	1095:1105	Isothermal calorimetry showed that compared with rHEL1, rSHL1 interacted more strongly with transferrin, a fucosylated glycoprotein, than with other fucosylated disaccharide glycoconjugates.
31931062	7	9	theme	fucosylated	1110:1120	arg1	glycoprotein					1122:1133	a fucosylated glycoprotein	1108:1133	a fucosylated glycoprotein	1108:1133	Isothermal calorimetry showed that compared with rHEL1, rSHL1 interacted more strongly with transferrin, a fucosylated glycoprotein, than with other fucosylated disaccharide glycoconjugates.
31931062	7	10	theme	other	1146:1150	arg1	glycoconjugates					1177:1191	other fucosylated disaccharide glycoconjugates	1146:1191	other fucosylated disaccharide glycoconjugates	1146:1191	Isothermal calorimetry showed that compared with rHEL1, rSHL1 interacted more strongly with transferrin, a fucosylated glycoprotein, than with other fucosylated disaccharide glycoconjugates.
31931062	2	11	theme	Recombinant	282:292	arg1	HEL2					294:297	Recombinant HEL2	282:297	Recombinant HEL2	282:297	Recombinant HEL2 reportedly binds O-linked glycans, but recombinant HEL1 (rHEL1) has not been characterized.
31931062	1	12	theme	lectin	269:274	arg1	HEL2					276:279	the core 1 O-glycan-binding lectin HEL2	241:279	the core 1 O-glycan-binding lectin HEL2	241:279	The mushroom Hericium erinaceus contains isolectins, including the ricin B-like lectin HEL1 and the core 1 O-glycan-binding lectin HEL2.
31931062	0	13	from	mushrooms	41:49	arg1	orthologues					20:30	Ricin B-like lectin orthologues	0:30	Ricin B-like lectin orthologues from two mushrooms, Hericium erinaceus and Stereum hirsutum,	0:91	Ricin B-like lectin orthologues from two mushrooms, Hericium erinaceus and Stereum hirsutum, enable recognition of highly fucosylated N-glycans.
31931062	7	14	gly	glycoprotein	1122:1133	arg1	transferrin					1095:1105	transferrin	1095:1105	transferrin	1095:1105	Isothermal calorimetry showed that compared with rHEL1, rSHL1 interacted more strongly with transferrin, a fucosylated glycoprotein, than with other fucosylated disaccharide glycoconjugates.
31931062	7	14	gly	glycoprotein	1122:1133	arg1	glycoprotein					1122:1133	a fucosylated glycoprotein	1108:1133	a fucosylated glycoprotein	1108:1133	Isothermal calorimetry showed that compared with rHEL1, rSHL1 interacted more strongly with transferrin, a fucosylated glycoprotein, than with other fucosylated disaccharide glycoconjugates.
31931062	8	15	theme	carcinoma	1354:1362	arg1	cells					1364:1368	SW1116 human colorectal carcinoma cells	1330:1368	SW1116 human colorectal carcinoma cells	1330:1368	Finally, rSHL1 and rHEL1 were comparable in their ability to detect highly fucosylated N-glycans within glycoproteins on the surface of SW1116 human colorectal carcinoma cells.
31931062	8	16	from	comparable	1224:1233	arg1	ability					1244:1250	their ability to detect highly fucosylated N-glycans within glycoproteins on the surface of SW1116 human colorectal carcinoma cells	1238:1368	their ability to detect highly fucosylated N-glycans within glycoproteins on the surface of SW1116 human colorectal carcinoma cells	1238:1368	Finally, rSHL1 and rHEL1 were comparable in their ability to detect highly fucosylated N-glycans within glycoproteins on the surface of SW1116 human colorectal carcinoma cells.
31931062	5	17	theme	rSHL1	744:748	arg1	activity					732:739	The hemagglutination activity	711:739	The hemagglutination activity of rSHL1	711:748	The hemagglutination activity of rSHL1 was inhibited by N-linked glycoprotein transferrin.
31931062	5	18	theme	glycoprotein	776:787	arg1	transferrin					789:799	N-linked glycoprotein transferrin	767:799	N-linked glycoprotein transferrin	767:799	The hemagglutination activity of rSHL1 was inhibited by N-linked glycoprotein transferrin.
31931062	9	19	theme	B-like	1394:1399	arg1	lectins					1401:1407	these ricin B-like lectins	1382:1407	these ricin B-like lectins	1382:1407	Therefore, these ricin B-like lectins might enable detection of highly fucosylated glycoepitopes on cancer cells for diagnostic applications.
31931062	0	20	theme	B-like	6:11	arg1	orthologues					20:30	Ricin B-like lectin orthologues	0:30	Ricin B-like lectin orthologues from two mushrooms, Hericium erinaceus and Stereum hirsutum,	0:91	Ricin B-like lectin orthologues from two mushrooms, Hericium erinaceus and Stereum hirsutum, enable recognition of highly fucosylated N-glycans.
31931062	3	21	dep	Stereum	400:406	arg1	hirsutum					408:415	hirsutum	408:415	hirsutum	408:415	HEL1 and Stereum hirsutum lectin (SHL1) orthologues, which contain the typical (QxW)3 ricin-B like motif, were evaluated.
31931062	4	22	theme	agglutination	687:699	arg1	activity					701:708	no agglutination activity	684:708	no agglutination activity	684:708	Interestingly, under non-denaturing conditions, recombinant SHL1 (rSHL1) existed as a trimer and exhibited agglutination activity, whereas rHEL1 existed as a monomer with no agglutination activity.
31931062	6	23	theme	different	872:880	arg1	intensities					890:900	different binding intensities	872:900	different binding intensities	872:900	A glycan-array analysis revealed that the two recombinant lectins had different binding intensities toward fucosylated N-glycans harboring fucose-α(1,2) galactose or fucose-α(1,4) N-acetylglucosamine.
31931062	3	24	theme	lectin	417:422	arg1	orthologues					431:441	HEL1 and Stereum hirsutum lectin (SHL1) orthologues	391:441	HEL1 and Stereum hirsutum lectin (SHL1) orthologues	391:441	HEL1 and Stereum hirsutum lectin (SHL1) orthologues, which contain the typical (QxW)3 ricin-B like motif, were evaluated.
31931062	0	25	theme	Ricin	0:4	arg1	orthologues					20:30	Ricin B-like lectin orthologues	0:30	Ricin B-like lectin orthologues from two mushrooms, Hericium erinaceus and Stereum hirsutum,	0:91	Ricin B-like lectin orthologues from two mushrooms, Hericium erinaceus and Stereum hirsutum, enable recognition of highly fucosylated N-glycans.
31931062	6	26	theme	fucose-α	968:975	arg1	N-acetylglucosamine					982:1000	fucose-α(1,4) N-acetylglucosamine	968:1000	fucose-α(1,4) N-acetylglucosamine	968:1000	A glycan-array analysis revealed that the two recombinant lectins had different binding intensities toward fucosylated N-glycans harboring fucose-α(1,2) galactose or fucose-α(1,4) N-acetylglucosamine.
31931062	9	27	theme	glycoepitopes	1454:1466	arg1	detection					1422:1430	detection	1422:1430	detection of highly fucosylated glycoepitopes on cancer cells	1422:1482	Therefore, these ricin B-like lectins might enable detection of highly fucosylated glycoepitopes on cancer cells for diagnostic applications.
31931062	2	28	theme	O-linked	316:323	arg1	glycans					325:331	O-linked glycans	316:331	O-linked glycans	316:331	Recombinant HEL2 reportedly binds O-linked glycans, but recombinant HEL1 (rHEL1) has not been characterized.
31931062	8	29	theme	fucosylated	1269:1279	arg1	N-glycans					1281:1289	highly fucosylated N-glycans	1262:1289	highly fucosylated N-glycans within glycoproteins on the surface of SW1116 human colorectal carcinoma cells	1262:1368	Finally, rSHL1 and rHEL1 were comparable in their ability to detect highly fucosylated N-glycans within glycoproteins on the surface of SW1116 human colorectal carcinoma cells.
31931062	4	30	theme	agglutination	620:632	arg1	trimer					599:604	a trimer	597:604	a trimer	597:604	Interestingly, under non-denaturing conditions, recombinant SHL1 (rSHL1) existed as a trimer and exhibited agglutination activity, whereas rHEL1 existed as a monomer with no agglutination activity.
31931062	4	30	theme	agglutination	620:632	arg1	activity					634:641	agglutination activity	620:641	agglutination activity	620:641	Interestingly, under non-denaturing conditions, recombinant SHL1 (rSHL1) existed as a trimer and exhibited agglutination activity, whereas rHEL1 existed as a monomer with no agglutination activity.
31931062	7	31	theme	disaccharide	1164:1175	arg1	glycoconjugates					1177:1191	other fucosylated disaccharide glycoconjugates	1146:1191	other fucosylated disaccharide glycoconjugates	1146:1191	Isothermal calorimetry showed that compared with rHEL1, rSHL1 interacted more strongly with transferrin, a fucosylated glycoprotein, than with other fucosylated disaccharide glycoconjugates.
31931062	9	32	theme	cancer	1471:1476	arg1	cells					1478:1482	cancer cells	1471:1482	cancer cells	1471:1482	Therefore, these ricin B-like lectins might enable detection of highly fucosylated glycoepitopes on cancer cells for diagnostic applications.
31931062	8	33	theme	colorectal	1343:1352	arg1	cells					1364:1368	SW1116 human colorectal carcinoma cells	1330:1368	SW1116 human colorectal carcinoma cells	1330:1368	Finally, rSHL1 and rHEL1 were comparable in their ability to detect highly fucosylated N-glycans within glycoproteins on the surface of SW1116 human colorectal carcinoma cells.
31931062	7	34	gly	fucosylated	1152:1162	arg1	glycoconjugates					1177:1191	other fucosylated disaccharide glycoconjugates	1146:1191	other fucosylated disaccharide glycoconjugates	1146:1191	Isothermal calorimetry showed that compared with rHEL1, rSHL1 interacted more strongly with transferrin, a fucosylated glycoprotein, than with other fucosylated disaccharide glycoconjugates.
31931062	0	35	theme	lectin	13:18	arg1	orthologues					20:30	Ricin B-like lectin orthologues	0:30	Ricin B-like lectin orthologues from two mushrooms, Hericium erinaceus and Stereum hirsutum,	0:91	Ricin B-like lectin orthologues from two mushrooms, Hericium erinaceus and Stereum hirsutum, enable recognition of highly fucosylated N-glycans.
31931062	9	36	theme	fucosylated	1442:1452	arg1	glycoepitopes					1454:1466	highly fucosylated glycoepitopes	1435:1466	highly fucosylated glycoepitopes	1435:1466	Therefore, these ricin B-like lectins might enable detection of highly fucosylated glycoepitopes on cancer cells for diagnostic applications.
31931062	6	37	theme	recombinant	848:858	arg1	lectins					860:866	the two recombinant lectins	840:866	the two recombinant lectins	840:866	A glycan-array analysis revealed that the two recombinant lectins had different binding intensities toward fucosylated N-glycans harboring fucose-α(1,2) galactose or fucose-α(1,4) N-acetylglucosamine.
31931062	3	38	theme	QxW	471:473	arg1	ricin-B					477:483	the typical (QxW)3 ricin-B	458:483	the typical (QxW)3 ricin-B like motif	458:494	HEL1 and Stereum hirsutum lectin (SHL1) orthologues, which contain the typical (QxW)3 ricin-B like motif, were evaluated.
31931062	6	39	theme	fucosylated	909:919	arg1	N-glycans					921:929	fucosylated N-glycans	909:929	fucosylated N-glycans harboring fucose-α(1,2) galactose or fucose-α(1,4) N-acetylglucosamine	909:1000	A glycan-array analysis revealed that the two recombinant lectins had different binding intensities toward fucosylated N-glycans harboring fucose-α(1,2) galactose or fucose-α(1,4) N-acetylglucosamine.
31931062	6	40	contain	had	868:870	arg1	lectins					860:866	the two recombinant lectins	840:866	the two recombinant lectins	840:866	A glycan-array analysis revealed that the two recombinant lectins had different binding intensities toward fucosylated N-glycans harboring fucose-α(1,2) galactose or fucose-α(1,4) N-acetylglucosamine.
31931062	6	40	contain	had	868:870	arg2	intensities					890:900	different binding intensities	872:900	different binding intensities	872:900	A glycan-array analysis revealed that the two recombinant lectins had different binding intensities toward fucosylated N-glycans harboring fucose-α(1,2) galactose or fucose-α(1,4) N-acetylglucosamine.
31931062	3	41	theme	Stereum	400:406	arg1	SHL1					425:428	SHL1	425:428	SHL1	425:428	HEL1 and Stereum hirsutum lectin (SHL1) orthologues, which contain the typical (QxW)3 ricin-B like motif, were evaluated.
31931062	3	41	theme	Stereum	400:406	arg1	lectin					417:422	Stereum hirsutum lectin	400:422	Stereum hirsutum lectin (SHL1)	400:429	HEL1 and Stereum hirsutum lectin (SHL1) orthologues, which contain the typical (QxW)3 ricin-B like motif, were evaluated.
31931062	2	42	theme	recombinant	338:348	arg1	rHEL1					356:360	rHEL1	356:360	rHEL1	356:360	Recombinant HEL2 reportedly binds O-linked glycans, but recombinant HEL1 (rHEL1) has not been characterized.
31931062	2	42	theme	recombinant	338:348	arg1	HEL1					350:353	recombinant HEL1	338:353	recombinant HEL1 (rHEL1)	338:361	Recombinant HEL2 reportedly binds O-linked glycans, but recombinant HEL1 (rHEL1) has not been characterized.
31931062	0	43	theme	N-glycans	134:142	arg1	recognition					100:110	recognition	100:110	recognition of highly fucosylated N-glycans	100:142	Ricin B-like lectin orthologues from two mushrooms, Hericium erinaceus and Stereum hirsutum, enable recognition of highly fucosylated N-glycans.
31931062	7	44	gly	fucosylated	1110:1120	arg1	transferrin					1095:1105	transferrin	1095:1105	transferrin	1095:1105	Isothermal calorimetry showed that compared with rHEL1, rSHL1 interacted more strongly with transferrin, a fucosylated glycoprotein, than with other fucosylated disaccharide glycoconjugates.
31931062	7	44	gly	fucosylated	1110:1120	arg1	glycoprotein					1122:1133	a fucosylated glycoprotein	1108:1133	a fucosylated glycoprotein	1108:1133	Isothermal calorimetry showed that compared with rHEL1, rSHL1 interacted more strongly with transferrin, a fucosylated glycoprotein, than with other fucosylated disaccharide glycoconjugates.
31931062	5	45	gly	glycoprotein	776:787	arg1	glycoprotein					776:787	N-linked glycoprotein transferrin	767:799	N-linked glycoprotein transferrin	767:799	The hemagglutination activity of rSHL1 was inhibited by N-linked glycoprotein transferrin.
31931062	9	46	theme	diagnostic	1488:1497	arg1	applications					1499:1510	diagnostic applications	1488:1510	diagnostic applications	1488:1510	Therefore, these ricin B-like lectins might enable detection of highly fucosylated glycoepitopes on cancer cells for diagnostic applications.
31931062	1	47	theme	ricin	212:216	arg1	HEL1					232:235	the ricin B-like lectin HEL1	208:235	the ricin B-like lectin HEL1	208:235	The mushroom Hericium erinaceus contains isolectins, including the ricin B-like lectin HEL1 and the core 1 O-glycan-binding lectin HEL2.
31931062	7	48	theme	fucosylated	1152:1162	arg1	glycoconjugates					1177:1191	other fucosylated disaccharide glycoconjugates	1146:1191	other fucosylated disaccharide glycoconjugates	1146:1191	Isothermal calorimetry showed that compared with rHEL1, rSHL1 interacted more strongly with transferrin, a fucosylated glycoprotein, than with other fucosylated disaccharide glycoconjugates.
31931062	6	49	theme	1,2	950:952	arg1	galactose					955:963	fucose-α(1,2) galactose	941:963	fucose-α(1,2) galactose	941:963	A glycan-array analysis revealed that the two recombinant lectins had different binding intensities toward fucosylated N-glycans harboring fucose-α(1,2) galactose or fucose-α(1,4) N-acetylglucosamine.
31931062	0	50	theme	fucosylated	122:132	arg1	N-glycans					134:142	highly fucosylated N-glycans	115:142	highly fucosylated N-glycans	115:142	Ricin B-like lectin orthologues from two mushrooms, Hericium erinaceus and Stereum hirsutum, enable recognition of highly fucosylated N-glycans.
31931062	1	51	contain	contains	177:184	arg2	HEL2					276:279	the core 1 O-glycan-binding lectin HEL2	241:279	the core 1 O-glycan-binding lectin HEL2	241:279	The mushroom Hericium erinaceus contains isolectins, including the ricin B-like lectin HEL1 and the core 1 O-glycan-binding lectin HEL2.
31931062	1	51	contain	contains	177:184	arg1	erinaceus					167:175	The mushroom Hericium erinaceus	145:175	The mushroom Hericium erinaceus	145:175	The mushroom Hericium erinaceus contains isolectins, including the ricin B-like lectin HEL1 and the core 1 O-glycan-binding lectin HEL2.
31931062	1	51	contain	contains	177:184	arg2	HEL1					232:235	the ricin B-like lectin HEL1	208:235	the ricin B-like lectin HEL1	208:235	The mushroom Hericium erinaceus contains isolectins, including the ricin B-like lectin HEL1 and the core 1 O-glycan-binding lectin HEL2.
31931062	1	51	contain	contains	177:184	arg2	isolectins					186:195	isolectins	186:195	isolectins	186:195	The mushroom Hericium erinaceus contains isolectins, including the ricin B-like lectin HEL1 and the core 1 O-glycan-binding lectin HEL2.
31931062	1	52	theme	B-like	218:223	arg1	HEL1					232:235	the ricin B-like lectin HEL1	208:235	the ricin B-like lectin HEL1	208:235	The mushroom Hericium erinaceus contains isolectins, including the ricin B-like lectin HEL1 and the core 1 O-glycan-binding lectin HEL2.
31931062	3	53	theme	HEL1	391:394	arg1	orthologues					431:441	HEL1 and Stereum hirsutum lectin (SHL1) orthologues	391:441	HEL1 and Stereum hirsutum lectin (SHL1) orthologues	391:441	HEL1 and Stereum hirsutum lectin (SHL1) orthologues, which contain the typical (QxW)3 ricin-B like motif, were evaluated.
31931062	9	54	theme	ricin	1388:1392	arg1	lectins					1401:1407	these ricin B-like lectins	1382:1407	these ricin B-like lectins	1382:1407	Therefore, these ricin B-like lectins might enable detection of highly fucosylated glycoepitopes on cancer cells for diagnostic applications.
31931062	9	55	from	detection	1422:1430	arg1	cells					1478:1482	cancer cells	1471:1482	cancer cells	1471:1482	Therefore, these ricin B-like lectins might enable detection of highly fucosylated glycoepitopes on cancer cells for diagnostic applications.
31931062	5	56	theme	hemagglutination	715:730	arg1	activity					732:739	The hemagglutination activity	711:739	The hemagglutination activity of rSHL1	711:748	The hemagglutination activity of rSHL1 was inhibited by N-linked glycoprotein transferrin.
31931062	6	57	theme	fucose-α	941:948	arg1	galactose					955:963	fucose-α(1,2) galactose	941:963	fucose-α(1,2) galactose	941:963	A glycan-array analysis revealed that the two recombinant lectins had different binding intensities toward fucosylated N-glycans harboring fucose-α(1,2) galactose or fucose-α(1,4) N-acetylglucosamine.
31931062	4	58	theme	recombinant	561:571	arg1	rSHL1					579:583	rSHL1	579:583	rSHL1	579:583	Interestingly, under non-denaturing conditions, recombinant SHL1 (rSHL1) existed as a trimer and exhibited agglutination activity, whereas rHEL1 existed as a monomer with no agglutination activity.
31931062	4	58	theme	recombinant	561:571	arg1	SHL1					573:576	recombinant SHL1	561:576	recombinant SHL1 (rSHL1)	561:584	Interestingly, under non-denaturing conditions, recombinant SHL1 (rSHL1) existed as a trimer and exhibited agglutination activity, whereas rHEL1 existed as a monomer with no agglutination activity.
31931062	3	59	contain	contain	450:456	arg2	ricin-B					477:483	the typical (QxW)3 ricin-B	458:483	the typical (QxW)3 ricin-B like motif	458:494	HEL1 and Stereum hirsutum lectin (SHL1) orthologues, which contain the typical (QxW)3 ricin-B like motif, were evaluated.
31931062	3	59	contain	contain	450:456	arg1	orthologues					431:441	HEL1 and Stereum hirsutum lectin (SHL1) orthologues	391:441	HEL1 and Stereum hirsutum lectin (SHL1) orthologues	391:441	HEL1 and Stereum hirsutum lectin (SHL1) orthologues, which contain the typical (QxW)3 ricin-B like motif, were evaluated.
31931062	2	60	link	O-linked	316:323	arg1	glycans					325:331	O-linked glycans	316:331	O-linked glycans	316:331	Recombinant HEL2 reportedly binds O-linked glycans, but recombinant HEL1 (rHEL1) has not been characterized.
31931062	9	61	gly	fucosylated	1442:1452	arg1	glycoepitopes					1454:1466	highly fucosylated glycoepitopes	1435:1466	highly fucosylated glycoepitopes	1435:1466	Therefore, these ricin B-like lectins might enable detection of highly fucosylated glycoepitopes on cancer cells for diagnostic applications.
31931062	7	62	theme	Isothermal	1003:1012	arg1	calorimetry					1014:1024	Isothermal calorimetry	1003:1024	Isothermal calorimetry	1003:1024	Isothermal calorimetry showed that compared with rHEL1, rSHL1 interacted more strongly with transferrin, a fucosylated glycoprotein, than with other fucosylated disaccharide glycoconjugates.
31931062	0	63	gly	fucosylated	122:132	arg1	N-glycans					134:142	highly fucosylated N-glycans	115:142	highly fucosylated N-glycans	115:142	Ricin B-like lectin orthologues from two mushrooms, Hericium erinaceus and Stereum hirsutum, enable recognition of highly fucosylated N-glycans.
31931062	1	64	theme	lectin	225:230	arg1	HEL1					232:235	the ricin B-like lectin HEL1	208:235	the ricin B-like lectin HEL1	208:235	The mushroom Hericium erinaceus contains isolectins, including the ricin B-like lectin HEL1 and the core 1 O-glycan-binding lectin HEL2.
31931062	8	65	from	ability	1244:1250	arg1	comparable					1224:1233	comparable	1224:1233	comparable	1224:1233	Finally, rSHL1 and rHEL1 were comparable in their ability to detect highly fucosylated N-glycans within glycoproteins on the surface of SW1116 human colorectal carcinoma cells.
31931062	6	66	theme	glycan-array	804:815	arg1	analysis					817:824	A glycan-array analysis	802:824	A glycan-array analysis	802:824	A glycan-array analysis revealed that the two recombinant lectins had different binding intensities toward fucosylated N-glycans harboring fucose-α(1,2) galactose or fucose-α(1,4) N-acetylglucosamine.
31931062	3	67	theme	typical	462:468	arg1	ricin-B					477:483	the typical (QxW)3 ricin-B	458:483	the typical (QxW)3 ricin-B like motif	458:494	HEL1 and Stereum hirsutum lectin (SHL1) orthologues, which contain the typical (QxW)3 ricin-B like motif, were evaluated.
31931062	8	68	theme	cells	1364:1368	arg1	surface					1319:1325	the surface	1315:1325	the surface of SW1116 human colorectal carcinoma cells	1315:1368	Finally, rSHL1 and rHEL1 were comparable in their ability to detect highly fucosylated N-glycans within glycoproteins on the surface of SW1116 human colorectal carcinoma cells.
31931062	8	69	gly	glycoproteins	1298:1310	arg1	glycoproteins					1298:1310	glycoproteins	1298:1310	glycoproteins	1298:1310	Finally, rSHL1 and rHEL1 were comparable in their ability to detect highly fucosylated N-glycans within glycoproteins on the surface of SW1116 human colorectal carcinoma cells.
31931062	5	70	link	N-linked	767:774	arg1	transferrin					789:799	N-linked glycoprotein transferrin	767:799	N-linked glycoprotein transferrin	767:799	The hemagglutination activity of rSHL1 was inhibited by N-linked glycoprotein transferrin.
31931062	8	71	theme	human	1337:1341	arg1	cells					1364:1368	SW1116 human colorectal carcinoma cells	1330:1368	SW1116 human colorectal carcinoma cells	1330:1368	Finally, rSHL1 and rHEL1 were comparable in their ability to detect highly fucosylated N-glycans within glycoproteins on the surface of SW1116 human colorectal carcinoma cells.
31931062	0	72	theme	Hericium	52:59	arg1	mushrooms					41:49	two mushrooms	37:49	two mushrooms	37:49	Ricin B-like lectin orthologues from two mushrooms, Hericium erinaceus and Stereum hirsutum, enable recognition of highly fucosylated N-glycans.
31931062	0	72	theme	Hericium	52:59	arg1	erinaceus					61:69	Hericium erinaceus	52:69	Hericium erinaceus	52:69	Ricin B-like lectin orthologues from two mushrooms, Hericium erinaceus and Stereum hirsutum, enable recognition of highly fucosylated N-glycans.
31931062	8	73	from	N-glycans	1281:1289	arg1	surface					1319:1325	the surface	1315:1325	the surface of SW1116 human colorectal carcinoma cells	1315:1368	Finally, rSHL1 and rHEL1 were comparable in their ability to detect highly fucosylated N-glycans within glycoproteins on the surface of SW1116 human colorectal carcinoma cells.
31931062	5	74	theme	N-linked	767:774	arg1	transferrin					789:799	N-linked glycoprotein transferrin	767:799	N-linked glycoprotein transferrin	767:799	The hemagglutination activity of rSHL1 was inhibited by N-linked glycoprotein transferrin.
31931062	6	75	gly	fucosylated	909:919	arg1	N-glycans					921:929	fucosylated N-glycans	909:929	fucosylated N-glycans harboring fucose-α(1,2) galactose or fucose-α(1,4) N-acetylglucosamine	909:1000	A glycan-array analysis revealed that the two recombinant lectins had different binding intensities toward fucosylated N-glycans harboring fucose-α(1,2) galactose or fucose-α(1,4) N-acetylglucosamine.
32240990	0	0	theme	fibrous	85:91	arg1	niche					93:97	aligned fibrous niche	77:97	aligned fibrous niche	77:97	Biofabrication of nerve fibers with mimetic myelin sheath-like structure and aligned fibrous niche.
32240990	2	1	theme	first	349:353	arg1	time					355:358	the first time	345:358	the first time	345:358	Here, we report, for the first time, a ready approach to fabricate biomimetic nerve fibers which are oriented and have a core-shell structure to spatially encapsulate two types of cells, neurons and Schwann cells.
32240990	9	2	theme	fibers	1512:1517	arg1	fabrication					1488:1498	the fabrication	1484:1498	the fabrication of oriented fibers with different types of cells separately encapsulated	1484:1571	Moreover, the present methodology on the fabrication of oriented fibers with different types of cells separately encapsulated should be applicable to biomimetic constructions of various tissues.
32240990	0	3	theme	aligned	77:83	arg1	niche					93:97	aligned fibrous niche	77:97	aligned fibrous niche	77:97	Biofabrication of nerve fibers with mimetic myelin sheath-like structure and aligned fibrous niche.
32240990	9	4	theme	various	1625:1631	arg1	tissues					1633:1639	various tissues	1625:1639	various tissues	1625:1639	Moreover, the present methodology on the fabrication of oriented fibers with different types of cells separately encapsulated should be applicable to biomimetic constructions of various tissues.
32240990	6	5	theme	fibers	1060:1065	arg1	core					1038:1041	the core	1034:1041	the core	1034:1041	Also, neurons and Schwann cells, which were respectively located in the core and shell of the fibers, displayed advanced biologic functions, including neurogenesis and myelinating maturation.
32240990	6	5	theme	fibers	1060:1065	arg1	shell					1047:1051	shell	1047:1051	shell	1047:1051	Also, neurons and Schwann cells, which were respectively located in the core and shell of the fibers, displayed advanced biologic functions, including neurogenesis and myelinating maturation.
32240990	5	6	theme	fibrin	879:884	arg1	compositions					835:846	the compositions	831:846	the compositions of methacrylate hyaluronan and fibrin	831:884	The orientation of the biomimetic nerve fibers was optimized by the control of the compositions of methacrylate hyaluronan and fibrin, together with the parameters of microfluidic shearing and external stretching.
32240990	1	7	contain	contain	114:120	arg2	fibers					151:156	hierarchically ordered nerve fibers	122:156	hierarchically ordered nerve fibers	122:156	Nerve tissues contain hierarchically ordered nerve fibers, while each of the nerve fibers has nano-oriented fibrous extracellular matrix and a core-shell structure of tubular myelin sheath with elongated axons encapsulated.
32240990	1	7	contain	contain	114:120	arg1	tissues					106:112	Nerve tissues	100:112	Nerve tissues	100:112	Nerve tissues contain hierarchically ordered nerve fibers, while each of the nerve fibers has nano-oriented fibrous extracellular matrix and a core-shell structure of tubular myelin sheath with elongated axons encapsulated.
32240990	1	8	theme	tubular	267:273	arg1	sheath					282:287	tubular myelin sheath	267:287	tubular myelin sheath	267:287	Nerve tissues contain hierarchically ordered nerve fibers, while each of the nerve fibers has nano-oriented fibrous extracellular matrix and a core-shell structure of tubular myelin sheath with elongated axons encapsulated.
32240990	2	9	theme	cells	504:508	arg1	types					495:499	two types	491:499	two types of cells, neurons and Schwann cells	491:535	Here, we report, for the first time, a ready approach to fabricate biomimetic nerve fibers which are oriented and have a core-shell structure to spatially encapsulate two types of cells, neurons and Schwann cells.
32240990	9	10	theme	oriented	1503:1510	arg1	fibers					1512:1517	oriented fibers	1503:1517	oriented fibers with different types of cells separately encapsulated	1503:1571	Moreover, the present methodology on the fabrication of oriented fibers with different types of cells separately encapsulated should be applicable to biomimetic constructions of various tissues.
32240990	6	11	from	shell	1047:1051	arg1	located					1023:1029	located	1023:1029	located	1023:1029	Also, neurons and Schwann cells, which were respectively located in the core and shell of the fibers, displayed advanced biologic functions, including neurogenesis and myelinating maturation.
32240990	5	12	theme	external	945:952	arg1	stretching					954:963	external stretching	945:963	external stretching	945:963	The orientation of the biomimetic nerve fibers was optimized by the control of the compositions of methacrylate hyaluronan and fibrin, together with the parameters of microfluidic shearing and external stretching.
32240990	1	13	theme	myelin	275:280	arg1	sheath					282:287	tubular myelin sheath	267:287	tubular myelin sheath	267:287	Nerve tissues contain hierarchically ordered nerve fibers, while each of the nerve fibers has nano-oriented fibrous extracellular matrix and a core-shell structure of tubular myelin sheath with elongated axons encapsulated.
32240990	9	14	with	fibers	1512:1517	arg1	types					1534:1538	different types	1524:1538	different types of cells separately encapsulated	1524:1571	Moreover, the present methodology on the fabrication of oriented fibers with different types of cells separately encapsulated should be applicable to biomimetic constructions of various tissues.
32240990	2	15	theme	nerve	402:406	arg1	fibers					408:413	biomimetic nerve fibers	391:413	biomimetic nerve fibers which are oriented and have a core-shell structure to spatially encapsulate two types of cells, neurons and Schwann cells	391:535	Here, we report, for the first time, a ready approach to fabricate biomimetic nerve fibers which are oriented and have a core-shell structure to spatially encapsulate two types of cells, neurons and Schwann cells.
32240990	5	16	theme	stretching	954:963	arg1	parameters					905:914	the parameters	901:914	the parameters of microfluidic shearing and external stretching	901:963	The orientation of the biomimetic nerve fibers was optimized by the control of the compositions of methacrylate hyaluronan and fibrin, together with the parameters of microfluidic shearing and external stretching.
32240990	2	17	theme	ready	363:367	arg1	approach					369:376	a ready approach	361:376	a ready approach to fabricate biomimetic nerve fibers which are oriented and have a core-shell structure to spatially encapsulate two types of cells, neurons and Schwann cells	361:535	Here, we report, for the first time, a ready approach to fabricate biomimetic nerve fibers which are oriented and have a core-shell structure to spatially encapsulate two types of cells, neurons and Schwann cells.
32240990	2	18	contain	have	438:441	arg2	structure					456:464	a core-shell structure	443:464	a core-shell structure to spatially encapsulate two types of cells, neurons and Schwann cells	443:535	Here, we report, for the first time, a ready approach to fabricate biomimetic nerve fibers which are oriented and have a core-shell structure to spatially encapsulate two types of cells, neurons and Schwann cells.
32240990	2	18	contain	have	438:441	arg1	fibers					408:413	biomimetic nerve fibers	391:413	biomimetic nerve fibers which are oriented and have a core-shell structure to spatially encapsulate two types of cells, neurons and Schwann cells	391:535	Here, we report, for the first time, a ready approach to fabricate biomimetic nerve fibers which are oriented and have a core-shell structure to spatially encapsulate two types of cells, neurons and Schwann cells.
32240990	6	19	theme	myelinating	1134:1144	arg1	maturation					1146:1155	myelinating maturation	1134:1155	myelinating maturation	1134:1155	Also, neurons and Schwann cells, which were respectively located in the core and shell of the fibers, displayed advanced biologic functions, including neurogenesis and myelinating maturation.
32240990	5	20	theme	biomimetic	775:784	arg1	fibers					792:797	the biomimetic nerve fibers	771:797	the biomimetic nerve fibers	771:797	The orientation of the biomimetic nerve fibers was optimized by the control of the compositions of methacrylate hyaluronan and fibrin, together with the parameters of microfluidic shearing and external stretching.
32240990	4	21	used	used	680:683	arg2	Alginate					667:674	Alginate	667:674	Alginate	667:674	Alginate was used first to assist the fabrication, which was washed away afterwards.
32240990	3	22	theme	microfluidic	540:551	arg1	system					553:558	A microfluidic system	538:558	A microfluidic system	538:558	A microfluidic system was designed and assembled, which contained a coaxial triple-channel chip and a stretching loading device.
32240990	8	23	from	potential	1388:1396	arg1	research					1414:1421	neuroscience research	1401:1421	neuroscience research	1401:1421	The present study brings insights to fabricate biomimetic nerve fibers for their potential in neuroscience research and nerve regeneration.
32240990	8	23	from	potential	1388:1396	arg1	regeneration					1433:1444	nerve regeneration	1427:1444	nerve regeneration	1427:1444	The present study brings insights to fabricate biomimetic nerve fibers for their potential in neuroscience research and nerve regeneration.
32240990	1	24	theme	nerve	177:181	arg1	fibers					183:188	the nerve fibers	173:188	the nerve fibers	173:188	Nerve tissues contain hierarchically ordered nerve fibers, while each of the nerve fibers has nano-oriented fibrous extracellular matrix and a core-shell structure of tubular myelin sheath with elongated axons encapsulated.
32240990	5	25	theme	nerve	786:790	arg1	fibers					792:797	the biomimetic nerve fibers	771:797	the biomimetic nerve fibers	771:797	The orientation of the biomimetic nerve fibers was optimized by the control of the compositions of methacrylate hyaluronan and fibrin, together with the parameters of microfluidic shearing and external stretching.
32240990	1	26	theme	sheath	282:287	arg1	matrix					230:235	nano-oriented fibrous extracellular matrix	194:235	nano-oriented fibrous extracellular matrix	194:235	Nerve tissues contain hierarchically ordered nerve fibers, while each of the nerve fibers has nano-oriented fibrous extracellular matrix and a core-shell structure of tubular myelin sheath with elongated axons encapsulated.
32240990	1	26	theme	sheath	282:287	arg1	structure					254:262	a core-shell structure	241:262	a core-shell structure of tubular myelin sheath with elongated axons encapsulated	241:321	Nerve tissues contain hierarchically ordered nerve fibers, while each of the nerve fibers has nano-oriented fibrous extracellular matrix and a core-shell structure of tubular myelin sheath with elongated axons encapsulated.
32240990	3	27	theme	stretching	640:649	arg1	device					659:664	a stretching loading device	638:664	a stretching loading device	638:664	A microfluidic system was designed and assembled, which contained a coaxial triple-channel chip and a stretching loading device.
32240990	8	28	theme	nerve	1365:1369	arg1	fibers					1371:1376	biomimetic nerve fibers	1354:1376	biomimetic nerve fibers	1354:1376	The present study brings insights to fabricate biomimetic nerve fibers for their potential in neuroscience research and nerve regeneration.
32240990	8	29	theme	biomimetic	1354:1363	arg1	fibers					1371:1376	biomimetic nerve fibers	1354:1376	biomimetic nerve fibers	1354:1376	The present study brings insights to fabricate biomimetic nerve fibers for their potential in neuroscience research and nerve regeneration.
32240990	9	30	theme	present	1461:1467	arg1	applicable					1583:1592	applicable	1583:1592	applicable	1583:1592	Moreover, the present methodology on the fabrication of oriented fibers with different types of cells separately encapsulated should be applicable to biomimetic constructions of various tissues.
32240990	9	30	theme	present	1461:1467	arg1	methodology					1469:1479	the present methodology	1457:1479	the present methodology on the fabrication of oriented fibers with different types of cells separately encapsulated	1457:1571	Moreover, the present methodology on the fabrication of oriented fibers with different types of cells separately encapsulated should be applicable to biomimetic constructions of various tissues.
32240990	0	31	theme	fibers	24:29	arg1	Biofabrication					0:13	Biofabrication	0:13	Biofabrication of nerve fibers with mimetic myelin sheath-like structure and aligned fibrous niche.	0:98	Biofabrication of nerve fibers with mimetic myelin sheath-like structure and aligned fibrous niche.
32240990	2	32	theme	cells	531:535	arg1	types					495:499	two types	491:499	two types of cells, neurons and Schwann cells	491:535	Here, we report, for the first time, a ready approach to fabricate biomimetic nerve fibers which are oriented and have a core-shell structure to spatially encapsulate two types of cells, neurons and Schwann cells.
32240990	6	33	from	located	1023:1029	arg1	core					1038:1041	the core	1034:1041	the core	1034:1041	Also, neurons and Schwann cells, which were respectively located in the core and shell of the fibers, displayed advanced biologic functions, including neurogenesis and myelinating maturation.
32240990	6	33	from	located	1023:1029	arg1	shell					1047:1051	shell	1047:1051	shell	1047:1051	Also, neurons and Schwann cells, which were respectively located in the core and shell of the fibers, displayed advanced biologic functions, including neurogenesis and myelinating maturation.
32240990	1	34	theme	Nerve	100:104	arg1	tissues					106:112	Nerve tissues	100:112	Nerve tissues	100:112	Nerve tissues contain hierarchically ordered nerve fibers, while each of the nerve fibers has nano-oriented fibrous extracellular matrix and a core-shell structure of tubular myelin sheath with elongated axons encapsulated.
32240990	6	35	from	core	1038:1041	arg1	located					1023:1029	located	1023:1029	located	1023:1029	Also, neurons and Schwann cells, which were respectively located in the core and shell of the fibers, displayed advanced biologic functions, including neurogenesis and myelinating maturation.
32240990	0	36	theme	nerve	18:22	arg1	fibers					24:29	nerve fibers	18:29	nerve fibers	18:29	Biofabrication of nerve fibers with mimetic myelin sheath-like structure and aligned fibrous niche.
32240990	1	37	theme	elongated	294:302	arg1	axons					304:308	elongated axons	294:308	elongated axons encapsulated	294:321	Nerve tissues contain hierarchically ordered nerve fibers, while each of the nerve fibers has nano-oriented fibrous extracellular matrix and a core-shell structure of tubular myelin sheath with elongated axons encapsulated.
32240990	2	38	theme	Schwann	523:529	arg1	cells					531:535	Schwann cells	523:535	Schwann cells	523:535	Here, we report, for the first time, a ready approach to fabricate biomimetic nerve fibers which are oriented and have a core-shell structure to spatially encapsulate two types of cells, neurons and Schwann cells.
32240990	6	39	theme	Schwann	984:990	arg1	cells					992:996	Schwann cells	984:996	Schwann cells	984:996	Also, neurons and Schwann cells, which were respectively located in the core and shell of the fibers, displayed advanced biologic functions, including neurogenesis and myelinating maturation.
32240990	5	40	theme	shearing	932:939	arg1	parameters					905:914	the parameters	901:914	the parameters of microfluidic shearing and external stretching	901:963	The orientation of the biomimetic nerve fibers was optimized by the control of the compositions of methacrylate hyaluronan and fibrin, together with the parameters of microfluidic shearing and external stretching.
32240990	9	41	theme	cells	1543:1547	arg1	types					1534:1538	different types	1524:1538	different types of cells separately encapsulated	1524:1571	Moreover, the present methodology on the fabrication of oriented fibers with different types of cells separately encapsulated should be applicable to biomimetic constructions of various tissues.
32240990	1	42	theme	nano-oriented	194:206	arg1	matrix					230:235	nano-oriented fibrous extracellular matrix	194:235	nano-oriented fibrous extracellular matrix	194:235	Nerve tissues contain hierarchically ordered nerve fibers, while each of the nerve fibers has nano-oriented fibrous extracellular matrix and a core-shell structure of tubular myelin sheath with elongated axons encapsulated.
32240990	0	43	theme	mimetic	36:42	arg1	structure					63:71	mimetic myelin sheath-like structure	36:71	mimetic myelin sheath-like structure	36:71	Biofabrication of nerve fibers with mimetic myelin sheath-like structure and aligned fibrous niche.
32240990	7	44	theme	single-layer	1281:1292	arg1	microfibers					1294:1304	single-layer microfibers	1281:1304	single-layer microfibers	1281:1304	We demonstrate that the neural performance is relatively good, compared to that resulted from individually encapsulated in single-layer microfibers.
32240990	9	45	theme	biomimetic	1597:1606	arg1	constructions					1608:1620	biomimetic constructions	1597:1620	biomimetic constructions of various tissues	1597:1639	Moreover, the present methodology on the fabrication of oriented fibers with different types of cells separately encapsulated should be applicable to biomimetic constructions of various tissues.
32240990	2	46	theme	biomimetic	391:400	arg1	fibers					408:413	biomimetic nerve fibers	391:413	biomimetic nerve fibers which are oriented and have a core-shell structure to spatially encapsulate two types of cells, neurons and Schwann cells	391:535	Here, we report, for the first time, a ready approach to fabricate biomimetic nerve fibers which are oriented and have a core-shell structure to spatially encapsulate two types of cells, neurons and Schwann cells.
32240990	8	47	theme	present	1311:1317	arg1	study					1319:1323	The present study	1307:1323	The present study	1307:1323	The present study brings insights to fabricate biomimetic nerve fibers for their potential in neuroscience research and nerve regeneration.
32240990	1	48	theme	fibrous	208:214	arg1	matrix					230:235	nano-oriented fibrous extracellular matrix	194:235	nano-oriented fibrous extracellular matrix	194:235	Nerve tissues contain hierarchically ordered nerve fibers, while each of the nerve fibers has nano-oriented fibrous extracellular matrix and a core-shell structure of tubular myelin sheath with elongated axons encapsulated.
32240990	2	49	theme	core-shell	445:454	arg1	structure					456:464	a core-shell structure	443:464	a core-shell structure to spatially encapsulate two types of cells, neurons and Schwann cells	443:535	Here, we report, for the first time, a ready approach to fabricate biomimetic nerve fibers which are oriented and have a core-shell structure to spatially encapsulate two types of cells, neurons and Schwann cells.
32240990	2	50	theme	neurons	511:517	arg1	types					495:499	two types	491:499	two types of cells, neurons and Schwann cells	491:535	Here, we report, for the first time, a ready approach to fabricate biomimetic nerve fibers which are oriented and have a core-shell structure to spatially encapsulate two types of cells, neurons and Schwann cells.
32240990	3	51	theme	coaxial	606:612	arg1	chip					629:632	a coaxial triple-channel chip	604:632	a coaxial triple-channel chip	604:632	A microfluidic system was designed and assembled, which contained a coaxial triple-channel chip and a stretching loading device.
32240990	5	52	theme	fibers	792:797	arg1	orientation					756:766	The orientation	752:766	The orientation of the biomimetic nerve fibers	752:797	The orientation of the biomimetic nerve fibers was optimized by the control of the compositions of methacrylate hyaluronan and fibrin, together with the parameters of microfluidic shearing and external stretching.
32240990	1	53	theme	extracellular	216:228	arg1	matrix					230:235	nano-oriented fibrous extracellular matrix	194:235	nano-oriented fibrous extracellular matrix	194:235	Nerve tissues contain hierarchically ordered nerve fibers, while each of the nerve fibers has nano-oriented fibrous extracellular matrix and a core-shell structure of tubular myelin sheath with elongated axons encapsulated.
32240990	0	54	theme	sheath-like	51:61	arg1	structure					63:71	mimetic myelin sheath-like structure	36:71	mimetic myelin sheath-like structure	36:71	Biofabrication of nerve fibers with mimetic myelin sheath-like structure and aligned fibrous niche.
32240990	5	55	theme	compositions	835:846	arg1	control					820:826	the control	816:826	the control of the compositions of methacrylate hyaluronan and fibrin	816:884	The orientation of the biomimetic nerve fibers was optimized by the control of the compositions of methacrylate hyaluronan and fibrin, together with the parameters of microfluidic shearing and external stretching.
32240990	3	56	theme	triple-channel	614:627	arg1	chip					629:632	a coaxial triple-channel chip	604:632	a coaxial triple-channel chip	604:632	A microfluidic system was designed and assembled, which contained a coaxial triple-channel chip and a stretching loading device.
32240990	9	57	theme	tissues	1633:1639	arg1	constructions					1608:1620	biomimetic constructions	1597:1620	biomimetic constructions of various tissues	1597:1639	Moreover, the present methodology on the fabrication of oriented fibers with different types of cells separately encapsulated should be applicable to biomimetic constructions of various tissues.
32240990	0	58	theme	myelin	44:49	arg1	structure					63:71	mimetic myelin sheath-like structure	36:71	mimetic myelin sheath-like structure	36:71	Biofabrication of nerve fibers with mimetic myelin sheath-like structure and aligned fibrous niche.
32240990	9	59	from	methodology	1469:1479	arg1	fabrication					1488:1498	the fabrication	1484:1498	the fabrication of oriented fibers with different types of cells separately encapsulated	1484:1571	Moreover, the present methodology on the fabrication of oriented fibers with different types of cells separately encapsulated should be applicable to biomimetic constructions of various tissues.
32240990	3	60	theme	loading	651:657	arg1	device					659:664	a stretching loading device	638:664	a stretching loading device	638:664	A microfluidic system was designed and assembled, which contained a coaxial triple-channel chip and a stretching loading device.
32240990	8	61	theme	neuroscience	1401:1412	arg1	research					1414:1421	neuroscience research	1401:1421	neuroscience research	1401:1421	The present study brings insights to fabricate biomimetic nerve fibers for their potential in neuroscience research and nerve regeneration.
32240990	1	62	with	structure	254:262	arg1	axons					304:308	elongated axons	294:308	elongated axons encapsulated	294:321	Nerve tissues contain hierarchically ordered nerve fibers, while each of the nerve fibers has nano-oriented fibrous extracellular matrix and a core-shell structure of tubular myelin sheath with elongated axons encapsulated.
32240990	6	63	theme	biologic	1087:1094	arg1	neurogenesis					1117:1128	neurogenesis	1117:1128	neurogenesis	1117:1128	Also, neurons and Schwann cells, which were respectively located in the core and shell of the fibers, displayed advanced biologic functions, including neurogenesis and myelinating maturation.
32240990	6	63	theme	biologic	1087:1094	arg1	maturation					1146:1155	myelinating maturation	1134:1155	myelinating maturation	1134:1155	Also, neurons and Schwann cells, which were respectively located in the core and shell of the fibers, displayed advanced biologic functions, including neurogenesis and myelinating maturation.
32240990	6	63	theme	biologic	1087:1094	arg1	functions					1096:1104	advanced biologic functions	1078:1104	advanced biologic functions	1078:1104	Also, neurons and Schwann cells, which were respectively located in the core and shell of the fibers, displayed advanced biologic functions, including neurogenesis and myelinating maturation.
32240990	8	64	theme	nerve	1427:1431	arg1	regeneration					1433:1444	nerve regeneration	1427:1444	nerve regeneration	1427:1444	The present study brings insights to fabricate biomimetic nerve fibers for their potential in neuroscience research and nerve regeneration.
32240990	0	65	with	Biofabrication	0:13	arg1	structure					63:71	mimetic myelin sheath-like structure	36:71	mimetic myelin sheath-like structure	36:71	Biofabrication of nerve fibers with mimetic myelin sheath-like structure and aligned fibrous niche.
32240990	0	65	with	Biofabrication	0:13	arg1	niche					93:97	aligned fibrous niche	77:97	aligned fibrous niche	77:97	Biofabrication of nerve fibers with mimetic myelin sheath-like structure and aligned fibrous niche.
32240990	1	66	theme	ordered	137:143	arg1	fibers					151:156	hierarchically ordered nerve fibers	122:156	hierarchically ordered nerve fibers	122:156	Nerve tissues contain hierarchically ordered nerve fibers, while each of the nerve fibers has nano-oriented fibrous extracellular matrix and a core-shell structure of tubular myelin sheath with elongated axons encapsulated.
32240990	5	67	theme	microfluidic	919:930	arg1	shearing					932:939	microfluidic shearing	919:939	microfluidic shearing	919:939	The orientation of the biomimetic nerve fibers was optimized by the control of the compositions of methacrylate hyaluronan and fibrin, together with the parameters of microfluidic shearing and external stretching.
32240990	7	68	theme	neural	1182:1187	arg1	performance					1189:1199	the neural performance	1178:1199	the neural performance	1178:1199	We demonstrate that the neural performance is relatively good, compared to that resulted from individually encapsulated in single-layer microfibers.
32240990	7	68	theme	neural	1182:1187	arg1	good					1215:1218	good	1215:1218	good	1215:1218	We demonstrate that the neural performance is relatively good, compared to that resulted from individually encapsulated in single-layer microfibers.
32240990	1	69	theme	nerve	145:149	arg1	fibers					151:156	hierarchically ordered nerve fibers	122:156	hierarchically ordered nerve fibers	122:156	Nerve tissues contain hierarchically ordered nerve fibers, while each of the nerve fibers has nano-oriented fibrous extracellular matrix and a core-shell structure of tubular myelin sheath with elongated axons encapsulated.
32240990	6	70	theme	advanced	1078:1085	arg1	neurogenesis					1117:1128	neurogenesis	1117:1128	neurogenesis	1117:1128	Also, neurons and Schwann cells, which were respectively located in the core and shell of the fibers, displayed advanced biologic functions, including neurogenesis and myelinating maturation.
32240990	6	70	theme	advanced	1078:1085	arg1	maturation					1146:1155	myelinating maturation	1134:1155	myelinating maturation	1134:1155	Also, neurons and Schwann cells, which were respectively located in the core and shell of the fibers, displayed advanced biologic functions, including neurogenesis and myelinating maturation.
32240990	6	70	theme	advanced	1078:1085	arg1	functions					1096:1104	advanced biologic functions	1078:1104	advanced biologic functions	1078:1104	Also, neurons and Schwann cells, which were respectively located in the core and shell of the fibers, displayed advanced biologic functions, including neurogenesis and myelinating maturation.
32240990	1	71	contain	has	190:192	arg2	matrix					230:235	nano-oriented fibrous extracellular matrix	194:235	nano-oriented fibrous extracellular matrix	194:235	Nerve tissues contain hierarchically ordered nerve fibers, while each of the nerve fibers has nano-oriented fibrous extracellular matrix and a core-shell structure of tubular myelin sheath with elongated axons encapsulated.
32240990	1	71	contain	has	190:192	arg1	fibers					183:188	the nerve fibers	173:188	the nerve fibers	173:188	Nerve tissues contain hierarchically ordered nerve fibers, while each of the nerve fibers has nano-oriented fibrous extracellular matrix and a core-shell structure of tubular myelin sheath with elongated axons encapsulated.
32240990	1	71	contain	has	190:192	arg2	structure					254:262	a core-shell structure	241:262	a core-shell structure of tubular myelin sheath with elongated axons encapsulated	241:321	Nerve tissues contain hierarchically ordered nerve fibers, while each of the nerve fibers has nano-oriented fibrous extracellular matrix and a core-shell structure of tubular myelin sheath with elongated axons encapsulated.
32240990	1	71	contain	has	190:192	arg1	each					165:168	each	165:168	each	165:168	Nerve tissues contain hierarchically ordered nerve fibers, while each of the nerve fibers has nano-oriented fibrous extracellular matrix and a core-shell structure of tubular myelin sheath with elongated axons encapsulated.
32240990	9	72	theme	different	1524:1532	arg1	types					1534:1538	different types	1524:1538	different types of cells separately encapsulated	1524:1571	Moreover, the present methodology on the fabrication of oriented fibers with different types of cells separately encapsulated should be applicable to biomimetic constructions of various tissues.
32240990	5	73	theme	methacrylate	851:862	arg1	hyaluronan					864:873	methacrylate hyaluronan	851:873	methacrylate hyaluronan	851:873	The orientation of the biomimetic nerve fibers was optimized by the control of the compositions of methacrylate hyaluronan and fibrin, together with the parameters of microfluidic shearing and external stretching.
32240990	6	74	located	located	1023:1029	arg1	core					1038:1041	the core	1034:1041	the core	1034:1041	Also, neurons and Schwann cells, which were respectively located in the core and shell of the fibers, displayed advanced biologic functions, including neurogenesis and myelinating maturation.
32240990	6	74	located	located	1023:1029	arg2	cells					992:996	Schwann cells	984:996	Schwann cells	984:996	Also, neurons and Schwann cells, which were respectively located in the core and shell of the fibers, displayed advanced biologic functions, including neurogenesis and myelinating maturation.
32240990	6	74	located	located	1023:1029	arg2	neurons					972:978	neurons	972:978	neurons	972:978	Also, neurons and Schwann cells, which were respectively located in the core and shell of the fibers, displayed advanced biologic functions, including neurogenesis and myelinating maturation.
32240990	6	74	located	located	1023:1029	arg1	shell					1047:1051	shell	1047:1051	shell	1047:1051	Also, neurons and Schwann cells, which were respectively located in the core and shell of the fibers, displayed advanced biologic functions, including neurogenesis and myelinating maturation.
32240990	3	75	contain	contained	594:602	arg2	device					659:664	a stretching loading device	638:664	a stretching loading device	638:664	A microfluidic system was designed and assembled, which contained a coaxial triple-channel chip and a stretching loading device.
32240990	3	75	contain	contained	594:602	arg1	which					588:592	which	588:592	which	588:592	A microfluidic system was designed and assembled, which contained a coaxial triple-channel chip and a stretching loading device.
32240990	3	75	contain	contained	594:602	arg2	chip					629:632	a coaxial triple-channel chip	604:632	a coaxial triple-channel chip	604:632	A microfluidic system was designed and assembled, which contained a coaxial triple-channel chip and a stretching loading device.
32240990	1	76	with	matrix	230:235	arg1	axons					304:308	elongated axons	294:308	elongated axons encapsulated	294:321	Nerve tissues contain hierarchically ordered nerve fibers, while each of the nerve fibers has nano-oriented fibrous extracellular matrix and a core-shell structure of tubular myelin sheath with elongated axons encapsulated.
32240990	5	77	theme	hyaluronan	864:873	arg1	compositions					835:846	the compositions	831:846	the compositions of methacrylate hyaluronan and fibrin	831:884	The orientation of the biomimetic nerve fibers was optimized by the control of the compositions of methacrylate hyaluronan and fibrin, together with the parameters of microfluidic shearing and external stretching.
32240990	1	78	theme	core-shell	243:252	arg1	structure					254:262	a core-shell structure	241:262	a core-shell structure of tubular myelin sheath with elongated axons encapsulated	241:321	Nerve tissues contain hierarchically ordered nerve fibers, while each of the nerve fibers has nano-oriented fibrous extracellular matrix and a core-shell structure of tubular myelin sheath with elongated axons encapsulated.
31535968	6	0	theme	2.0-3.0 	507:514	arg1	%					515:515	%	515:515	%	515:515	Optimal growth occurred at 28-30 °C, pH 7.0-7.5 and in the presence of 2.0-3.0 % (w/v) NaCl.
31535968	2	1	theme	aerobic	117:123	arg1	bacterium					147:155	A Gram-stain-negative, aerobic and curved-rod-shaped bacterium	94:155	A Gram-stain-negative, aerobic and curved-rod-shaped bacterium	94:155	A Gram-stain-negative, aerobic and curved-rod-shaped bacterium, designated QM202T, was isolated from red algae (Gracilaria blodgettii).
31535968	6	2	theme	Optimal	436:442	arg1	growth					444:449	Optimal growth	436:449	Optimal growth	436:449	Optimal growth occurred at 28-30 °C, pH 7.0-7.5 and in the presence of 2.0-3.0 % (w/v) NaCl.
31535968	13	3	theme	Marinomonas	1192:1202	arg1	sp					1216:1217	Marinomonas agarivorans sp	1192:1217	Marinomonas agarivorans sp	1192:1217	The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness indicated that strain QM202T can be considered to represent a novel species, Marinomonas agarivorans sp.
31535968	13	3	theme	Marinomonas	1192:1202	arg1	species					1183:1189	a novel species	1175:1189	a novel species	1175:1189	The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness indicated that strain QM202T can be considered to represent a novel species, Marinomonas agarivorans sp.
31535968	13	4	theme	novel	1177:1181	arg1	sp					1216:1217	Marinomonas agarivorans sp	1192:1217	Marinomonas agarivorans sp	1192:1217	The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness indicated that strain QM202T can be considered to represent a novel species, Marinomonas agarivorans sp.
31535968	13	4	theme	novel	1177:1181	arg1	species					1183:1189	a novel species	1175:1189	a novel species	1175:1189	The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness indicated that strain QM202T can be considered to represent a novel species, Marinomonas agarivorans sp.
31535968	11	5	theme	Phylogenetic	777:788	arg1	analysis					790:797	Phylogenetic analysis	777:797	Phylogenetic analysis based on 16S rRNA gene sequences	777:830	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain QM202T belonged to the genus Marinomonas.
31535968	13	6	theme	genetic	1091:1097	arg1	distinctiveness					1099:1113	the phylogenetic and genetic distinctiveness	1070:1113	the phylogenetic and genetic distinctiveness	1070:1113	The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness indicated that strain QM202T can be considered to represent a novel species, Marinomonas agarivorans sp.
31535968	12	7	from	neighbour	917:925	arg1	terms					930:934	terms	930:934	terms of 16S rRNA gene sequence identity	930:969	The closest described neighbour in terms of 16S rRNA gene sequence identity was Marinomonas blandensis MED121T (95.5 %).
31535968	10	8	theme	fatty	708:712	arg1	acids					714:718	The dominant fatty acids	695:718	The dominant fatty acids	695:718	The dominant fatty acids were C18:1ω7c, C16:0 and C16:1ω7c and/or iso-C15:0 2-OH.
31535968	10	8	theme	fatty	708:712	arg1	C16:0					735:739	C16:0	735:739	C16:0	735:739	The dominant fatty acids were C18:1ω7c, C16:0 and C16:1ω7c and/or iso-C15:0 2-OH.
31535968	2	9	theme	Gram-stain-negative	96:114	arg1	bacterium					147:155	A Gram-stain-negative, aerobic and curved-rod-shaped bacterium	94:155	A Gram-stain-negative, aerobic and curved-rod-shaped bacterium	94:155	A Gram-stain-negative, aerobic and curved-rod-shaped bacterium, designated QM202T, was isolated from red algae (Gracilaria blodgettii).
31535968	13	10	dep	together	1056:1063	arg1	with					1065:1068	with	1065:1068	with	1065:1068	The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness indicated that strain QM202T can be considered to represent a novel species, Marinomonas agarivorans sp.
31535968	7	11	theme	G+C	537:539	arg1	%					561:561	41.4 mol%	553:561	41.4 mol%	553:561	The DNA G+C content was 41.4 mol%.
31535968	7	11	theme	G+C	537:539	arg1	content					541:547	The DNA G+C content	529:547	The DNA G+C content	529:547	The DNA G+C content was 41.4 mol%.
31535968	5	12	theme	single	412:417	arg1	flagellum					425:433	a single polar flagellum	410:433	a single polar flagellum	410:433	It was motile by means of a single polar flagellum.
31535968	10	13	theme	dominant	699:706	arg1	acids					714:718	The dominant fatty acids	695:718	The dominant fatty acids	695:718	The dominant fatty acids were C18:1ω7c, C16:0 and C16:1ω7c and/or iso-C15:0 2-OH.
31535968	10	13	theme	dominant	699:706	arg1	C16:0					735:739	C16:0	735:739	C16:0	735:739	The dominant fatty acids were C18:1ω7c, C16:0 and C16:1ω7c and/or iso-C15:0 2-OH.
31535968	7	14	theme	mol	558:560	arg1	%					561:561	41.4 mol%	553:561	41.4 mol%	553:561	The DNA G+C content was 41.4 mol%.
31535968	7	14	theme	mol	558:560	arg1	content					541:547	The DNA G+C content	529:547	The DNA G+C content	529:547	The DNA G+C content was 41.4 mol%.
31535968	12	15	theme	described	907:915	arg1	neighbour					917:925	The closest described neighbour	895:925	The closest described neighbour in terms of 16S rRNA gene sequence identity	895:969	The closest described neighbour in terms of 16S rRNA gene sequence identity was Marinomonas blandensis MED121T (95.5 %).
31535968	12	15	theme	described	907:915	arg1	MED121T					998:1004	Marinomonas blandensis MED121T	975:1004	Marinomonas blandensis MED121T	975:1004	The closest described neighbour in terms of 16S rRNA gene sequence identity was Marinomonas blandensis MED121T (95.5 %).
31535968	11	16	theme	16S	808:810	arg1	sequences					822:830	16S rRNA gene sequences	808:830	16S rRNA gene sequences	808:830	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain QM202T belonged to the genus Marinomonas.
31535968	3	17	theme	strain	239:244	arg1	QM202T					246:251	strain QM202T	239:251	strain QM202T	239:251	Cells of strain QM202T were 0.2-0.3 µm wide and 1.0-2.5 µm long, catalase-negative and oxidase-positive.
31535968	12	18	theme	closest	899:905	arg1	neighbour					917:925	The closest described neighbour	895:925	The closest described neighbour in terms of 16S rRNA gene sequence identity	895:969	The closest described neighbour in terms of 16S rRNA gene sequence identity was Marinomonas blandensis MED121T (95.5 %).
31535968	12	18	theme	closest	899:905	arg1	MED121T					998:1004	Marinomonas blandensis MED121T	975:1004	Marinomonas blandensis MED121T	975:1004	The closest described neighbour in terms of 16S rRNA gene sequence identity was Marinomonas blandensis MED121T (95.5 %).
31535968	9	19	theme	predominant	668:678	arg1	Phophatidylethanolamine					610:632	Phophatidylethanolamine	610:632	Phophatidylethanolamine	610:632	Phophatidylethanolamine and phosphatidylglycerol were the predominant phospholipids.
31535968	9	19	theme	predominant	668:678	arg1	phospholipids					680:692	the predominant phospholipids	664:692	the predominant phospholipids	664:692	Phophatidylethanolamine and phosphatidylglycerol were the predominant phospholipids.
31535968	9	19	theme	predominant	668:678	arg1	phosphatidylglycerol					638:657	phosphatidylglycerol	638:657	phosphatidylglycerol	638:657	Phophatidylethanolamine and phosphatidylglycerol were the predominant phospholipids.
31535968	12	20	dep	MED121T	998:1004	arg1	%					1012:1012	95.5 %	1007:1012	95.5 %	1007:1012	The closest described neighbour in terms of 16S rRNA gene sequence identity was Marinomonas blandensis MED121T (95.5 %).
31535968	3	21	theme	QM202T	246:251	arg1	Cells					230:234	Cells	230:234	Cells of strain QM202T	230:251	Cells of strain QM202T were 0.2-0.3 µm wide and 1.0-2.5 µm long, catalase-negative and oxidase-positive.
31535968	4	22	theme	agar-degrading	359:372	arg1	activity					374:381	an agar-degrading activity	356:381	an agar-degrading activity	356:381	The strain exhibited an agar-degrading activity.
31535968	7	23	theme	DNA	533:535	arg1	%					561:561	41.4 mol%	553:561	41.4 mol%	553:561	The DNA G+C content was 41.4 mol%.
31535968	7	23	theme	DNA	533:535	arg1	content					541:547	The DNA G+C content	529:547	The DNA G+C content	529:547	The DNA G+C content was 41.4 mol%.
31535968	2	24	theme	curved-rod-shaped	129:145	arg1	bacterium					147:155	A Gram-stain-negative, aerobic and curved-rod-shaped bacterium	94:155	A Gram-stain-negative, aerobic and curved-rod-shaped bacterium	94:155	A Gram-stain-negative, aerobic and curved-rod-shaped bacterium, designated QM202T, was isolated from red algae (Gracilaria blodgettii).
31535968	1	25	theme	red	83:85	arg1	algae					87:91	red algae	83:91	red algae	83:91	nov., an agar-degrading marine bacterium isolated from red algae.
31535968	6	26	dep	%	515:515	arg1	w/v					518:520	w/v	518:520	w/v	518:520	Optimal growth occurred at 28-30 °C, pH 7.0-7.5 and in the presence of 2.0-3.0 % (w/v) NaCl.
31535968	13	27	theme	phylogenetic	1074:1085	arg1	distinctiveness					1099:1113	the phylogenetic and genetic distinctiveness	1070:1113	the phylogenetic and genetic distinctiveness	1070:1113	The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness indicated that strain QM202T can be considered to represent a novel species, Marinomonas agarivorans sp.
31535968	13	28	theme	differential	1020:1031	arg1	properties					1044:1053	The differential phenotypic properties	1016:1053	The differential phenotypic properties	1016:1053	The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness indicated that strain QM202T can be considered to represent a novel species, Marinomonas agarivorans sp.
31535968	10	29	theme	iso-C15:0	761:769	arg1	2-OH					771:774	iso-C15:0 2-OH	761:774	iso-C15:0 2-OH	761:774	The dominant fatty acids were C18:1ω7c, C16:0 and C16:1ω7c and/or iso-C15:0 2-OH.
31535968	11	30	theme	strain	846:851	arg1	QM202T					853:858	strain QM202T	846:858	strain QM202T	846:858	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain QM202T belonged to the genus Marinomonas.
31535968	2	31	attach	isolated	181:188	arg2	bacterium					147:155	A Gram-stain-negative, aerobic and curved-rod-shaped bacterium	94:155	A Gram-stain-negative, aerobic and curved-rod-shaped bacterium	94:155	A Gram-stain-negative, aerobic and curved-rod-shaped bacterium, designated QM202T, was isolated from red algae (Gracilaria blodgettii).
31535968	2	31	attach	isolated	181:188	arg1	algae					199:203	red algae	195:203	red algae (Gracilaria blodgettii)	195:227	A Gram-stain-negative, aerobic and curved-rod-shaped bacterium, designated QM202T, was isolated from red algae (Gracilaria blodgettii).
31535968	2	31	attach	isolated	181:188	arg1	blodgettii					217:226	Gracilaria blodgettii	206:226	Gracilaria blodgettii	206:226	A Gram-stain-negative, aerobic and curved-rod-shaped bacterium, designated QM202T, was isolated from red algae (Gracilaria blodgettii).
31535968	13	32	theme	phenotypic	1033:1042	arg1	properties					1044:1053	The differential phenotypic properties	1016:1053	The differential phenotypic properties	1016:1053	The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness indicated that strain QM202T can be considered to represent a novel species, Marinomonas agarivorans sp.
31535968	15	33	theme	52475T=MCCC	1258:1268	arg1	QM202T					1244:1249	QM202T	1244:1249	QM202T (=KCTC 52475T=MCCC 1H00145T)	1244:1278	The type strain is QM202T (=KCTC 52475T=MCCC 1H00145T).
31535968	15	33	theme	52475T=MCCC	1258:1268	arg1	1H00145T					1270:1277	=KCTC 52475T=MCCC 1H00145T	1252:1277	=KCTC 52475T=MCCC 1H00145T	1252:1277	The type strain is QM202T (=KCTC 52475T=MCCC 1H00145T).
31535968	6	34	theme	NaCl	523:526	arg1	presence					495:502	the presence	491:502	the presence of 2.0-3.0 % (w/v) NaCl	491:526	Optimal growth occurred at 28-30 °C, pH 7.0-7.5 and in the presence of 2.0-3.0 % (w/v) NaCl.
31535968	11	35	theme	rRNA	812:815	arg1	sequences					822:830	16S rRNA gene sequences	808:830	16S rRNA gene sequences	808:830	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain QM202T belonged to the genus Marinomonas.
31535968	15	36	theme	=KCTC	1252:1256	arg1	QM202T					1244:1249	QM202T	1244:1249	QM202T (=KCTC 52475T=MCCC 1H00145T)	1244:1278	The type strain is QM202T (=KCTC 52475T=MCCC 1H00145T).
31535968	15	36	theme	=KCTC	1252:1256	arg1	1H00145T					1270:1277	=KCTC 52475T=MCCC 1H00145T	1252:1277	=KCTC 52475T=MCCC 1H00145T	1252:1277	The type strain is QM202T (=KCTC 52475T=MCCC 1H00145T).
31535968	12	37	theme	rRNA	943:946	arg1	identity					962:969	16S rRNA gene sequence identity	939:969	16S rRNA gene sequence identity	939:969	The closest described neighbour in terms of 16S rRNA gene sequence identity was Marinomonas blandensis MED121T (95.5 %).
31535968	11	38	theme	gene	817:820	arg1	sequences					822:830	16S rRNA gene sequences	808:830	16S rRNA gene sequences	808:830	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain QM202T belonged to the genus Marinomonas.
31535968	12	39	theme	16S	939:941	arg1	rRNA					943:946	16S rRNA	939:946	16S rRNA gene sequence identity	939:969	The closest described neighbour in terms of 16S rRNA gene sequence identity was Marinomonas blandensis MED121T (95.5 %).
31535968	12	40	theme	identity	962:969	arg1	terms					930:934	terms	930:934	terms of 16S rRNA gene sequence identity	930:969	The closest described neighbour in terms of 16S rRNA gene sequence identity was Marinomonas blandensis MED121T (95.5 %).
31535968	2	41	theme	Gracilaria	206:215	arg1	algae					199:203	red algae	195:203	red algae (Gracilaria blodgettii)	195:227	A Gram-stain-negative, aerobic and curved-rod-shaped bacterium, designated QM202T, was isolated from red algae (Gracilaria blodgettii).
31535968	2	41	theme	Gracilaria	206:215	arg1	blodgettii					217:226	Gracilaria blodgettii	206:226	Gracilaria blodgettii	206:226	A Gram-stain-negative, aerobic and curved-rod-shaped bacterium, designated QM202T, was isolated from red algae (Gracilaria blodgettii).
31535968	12	42	theme	sequence	953:960	arg1	identity					962:969	16S rRNA gene sequence identity	939:969	16S rRNA gene sequence identity	939:969	The closest described neighbour in terms of 16S rRNA gene sequence identity was Marinomonas blandensis MED121T (95.5 %).
31535968	5	43	theme	polar	419:423	arg1	flagellum					425:433	a single polar flagellum	410:433	a single polar flagellum	410:433	It was motile by means of a single polar flagellum.
31535968	12	44	theme	blandensis	987:996	arg1	neighbour					917:925	The closest described neighbour	895:925	The closest described neighbour in terms of 16S rRNA gene sequence identity	895:969	The closest described neighbour in terms of 16S rRNA gene sequence identity was Marinomonas blandensis MED121T (95.5 %).
31535968	12	44	theme	blandensis	987:996	arg1	MED121T					998:1004	Marinomonas blandensis MED121T	975:1004	Marinomonas blandensis MED121T	975:1004	The closest described neighbour in terms of 16S rRNA gene sequence identity was Marinomonas blandensis MED121T (95.5 %).
31535968	15	45	theme	type	1229:1232	arg1	QM202T					1244:1249	QM202T	1244:1249	QM202T (=KCTC 52475T=MCCC 1H00145T)	1244:1278	The type strain is QM202T (=KCTC 52475T=MCCC 1H00145T).
31535968	15	45	theme	type	1229:1232	arg1	strain					1234:1239	The type strain	1225:1239	The type strain	1225:1239	The type strain is QM202T (=KCTC 52475T=MCCC 1H00145T).
31535968	12	46	theme	gene	948:951	arg1	identity					962:969	16S rRNA gene sequence identity	939:969	16S rRNA gene sequence identity	939:969	The closest described neighbour in terms of 16S rRNA gene sequence identity was Marinomonas blandensis MED121T (95.5 %).
31535968	11	47	theme	genus	876:880	arg1	Marinomonas					882:892	the genus Marinomonas	872:892	the genus Marinomonas	872:892	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain QM202T belonged to the genus Marinomonas.
31535968	12	48	theme	Marinomonas	975:985	arg1	neighbour					917:925	The closest described neighbour	895:925	The closest described neighbour in terms of 16S rRNA gene sequence identity	895:969	The closest described neighbour in terms of 16S rRNA gene sequence identity was Marinomonas blandensis MED121T (95.5 %).
31535968	12	48	theme	Marinomonas	975:985	arg1	MED121T					998:1004	Marinomonas blandensis MED121T	975:1004	Marinomonas blandensis MED121T	975:1004	The closest described neighbour in terms of 16S rRNA gene sequence identity was Marinomonas blandensis MED121T (95.5 %).
31535968	2	49	theme	red	195:197	arg1	algae					199:203	red algae	195:203	red algae (Gracilaria blodgettii)	195:227	A Gram-stain-negative, aerobic and curved-rod-shaped bacterium, designated QM202T, was isolated from red algae (Gracilaria blodgettii).
31535968	2	49	theme	red	195:197	arg1	blodgettii					217:226	Gracilaria blodgettii	206:226	Gracilaria blodgettii	206:226	A Gram-stain-negative, aerobic and curved-rod-shaped bacterium, designated QM202T, was isolated from red algae (Gracilaria blodgettii).
31535968	1	50	theme	marine	52:57	arg1	bacterium					59:67	an agar-degrading marine bacterium	34:67	an agar-degrading marine bacterium	34:67	nov., an agar-degrading marine bacterium isolated from red algae.
31535968	1	50	theme	marine	52:57	arg1	nov.					28:31	nov.	28:31	nov.	28:31	nov., an agar-degrading marine bacterium isolated from red algae.
31535968	13	51	theme	strain	1130:1135	arg1	QM202T					1137:1142	strain QM202T	1130:1142	strain QM202T	1130:1142	The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness indicated that strain QM202T can be considered to represent a novel species, Marinomonas agarivorans sp.
31535968	8	52	theme	isoprenoid	568:577	arg1	Q-8					605:607	Q-8	605:607	Q-8	605:607	The isoprenoid quinone was identified as Q-8.
31535968	8	52	theme	isoprenoid	568:577	arg1	quinone					579:585	The isoprenoid quinone	564:585	The isoprenoid quinone	564:585	The isoprenoid quinone was identified as Q-8.
31535968	6	53	theme	%	515:515	arg1	NaCl					523:526	2.0-3.0 % (w/v) NaCl	507:526	2.0-3.0 % (w/v) NaCl	507:526	Optimal growth occurred at 28-30 °C, pH 7.0-7.5 and in the presence of 2.0-3.0 % (w/v) NaCl.
31535968	13	54	theme	agarivorans	1204:1214	arg1	sp					1216:1217	Marinomonas agarivorans sp	1192:1217	Marinomonas agarivorans sp	1192:1217	The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness indicated that strain QM202T can be considered to represent a novel species, Marinomonas agarivorans sp.
31535968	13	54	theme	agarivorans	1204:1214	arg1	species					1183:1189	a novel species	1175:1189	a novel species	1175:1189	The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness indicated that strain QM202T can be considered to represent a novel species, Marinomonas agarivorans sp.
31535968	1	55	theme	agar-degrading	37:50	arg1	bacterium					59:67	an agar-degrading marine bacterium	34:67	an agar-degrading marine bacterium	34:67	nov., an agar-degrading marine bacterium isolated from red algae.
31535968	1	55	theme	agar-degrading	37:50	arg1	nov.					28:31	nov.	28:31	nov.	28:31	nov., an agar-degrading marine bacterium isolated from red algae.
34482786	8	0	theme	myricetin	1053:1061	arg1	3-O-β-D-galatopyranoside					1063:1086	myricetin 3-O-β-D-galatopyranoside	1053:1086	myricetin 3-O-β-D-galatopyranoside	1053:1086	High total phenolic and flavonoid contents were recorded in L. cercinense and myricitrin, myricetin, myricetin 3-O-β-D-galatopyranoside, luteolin 7-O-glucoside and rutin were the main phenolics in both species.
34482786	8	0	theme	myricetin	1053:1061	arg1	myricitrin					1030:1039	myricitrin	1030:1039	myricitrin	1030:1039	High total phenolic and flavonoid contents were recorded in L. cercinense and myricitrin, myricetin, myricetin 3-O-β-D-galatopyranoside, luteolin 7-O-glucoside and rutin were the main phenolics in both species.
34482786	8	0	theme	myricetin	1053:1061	arg1	phenolics					1136:1144	the main phenolics	1127:1144	the main phenolics in both species	1127:1160	High total phenolic and flavonoid contents were recorded in L. cercinense and myricitrin, myricetin, myricetin 3-O-β-D-galatopyranoside, luteolin 7-O-glucoside and rutin were the main phenolics in both species.
34482786	8	0	theme	myricetin	1053:1061	arg1	myricetin					1042:1050	myricetin	1042:1050	myricetin	1042:1050	High total phenolic and flavonoid contents were recorded in L. cercinense and myricitrin, myricetin, myricetin 3-O-β-D-galatopyranoside, luteolin 7-O-glucoside and rutin were the main phenolics in both species.
34482786	8	0	theme	myricetin	1053:1061	arg1	7-O-glucoside					1098:1110	luteolin 7-O-glucoside	1089:1110	luteolin 7-O-glucoside	1089:1110	High total phenolic and flavonoid contents were recorded in L. cercinense and myricitrin, myricetin, myricetin 3-O-β-D-galatopyranoside, luteolin 7-O-glucoside and rutin were the main phenolics in both species.
34482786	8	0	theme	myricetin	1053:1061	arg1	rutin					1116:1120	rutin	1116:1120	rutin	1116:1120	High total phenolic and flavonoid contents were recorded in L. cercinense and myricitrin, myricetin, myricetin 3-O-β-D-galatopyranoside, luteolin 7-O-glucoside and rutin were the main phenolics in both species.
34482786	8	1	theme	L.	1012:1013	arg1	cercinense					1015:1024	L. cercinense	1012:1024	L. cercinense	1012:1024	High total phenolic and flavonoid contents were recorded in L. cercinense and myricitrin, myricetin, myricetin 3-O-β-D-galatopyranoside, luteolin 7-O-glucoside and rutin were the main phenolics in both species.
34482786	2	2	theme	inhibitory	335:344	arg1	activities					346:355	the antioxidant, anti-inflammatory and enzyme inhibitory activities	289:355	the antioxidant, anti-inflammatory and enzyme inhibitory activities of two Tunisian endemic plants, L. boitardii and L. cercinense	289:418	This study investigates for the first time the antioxidant, anti-inflammatory and enzyme inhibitory activities of two Tunisian endemic plants, L. boitardii and L. cercinense.
34482786	0	3	theme	Erben	94:98	arg1	Bioactivities					0:12	Bioactivities	0:12	Bioactivities	0:12	Bioactivities and phenolic composition of Limonium boitardii Maire and L. cercinense Brullo & Erben (Plumbaginaceae): two Tunisian strict endemic plants.
34482786	0	3	theme	Erben	94:98	arg1	composition					27:37	phenolic composition	18:37	phenolic composition	18:37	Bioactivities and phenolic composition of Limonium boitardii Maire and L. cercinense Brullo & Erben (Plumbaginaceae): two Tunisian strict endemic plants.
34482786	8	4	theme	luteolin	1089:1096	arg1	3-O-β-D-galatopyranoside					1063:1086	myricetin 3-O-β-D-galatopyranoside	1053:1086	myricetin 3-O-β-D-galatopyranoside	1053:1086	High total phenolic and flavonoid contents were recorded in L. cercinense and myricitrin, myricetin, myricetin 3-O-β-D-galatopyranoside, luteolin 7-O-glucoside and rutin were the main phenolics in both species.
34482786	8	4	theme	luteolin	1089:1096	arg1	myricitrin					1030:1039	myricitrin	1030:1039	myricitrin	1030:1039	High total phenolic and flavonoid contents were recorded in L. cercinense and myricitrin, myricetin, myricetin 3-O-β-D-galatopyranoside, luteolin 7-O-glucoside and rutin were the main phenolics in both species.
34482786	8	4	theme	luteolin	1089:1096	arg1	phenolics					1136:1144	the main phenolics	1127:1144	the main phenolics in both species	1127:1160	High total phenolic and flavonoid contents were recorded in L. cercinense and myricitrin, myricetin, myricetin 3-O-β-D-galatopyranoside, luteolin 7-O-glucoside and rutin were the main phenolics in both species.
34482786	8	4	theme	luteolin	1089:1096	arg1	myricetin					1042:1050	myricetin	1042:1050	myricetin	1042:1050	High total phenolic and flavonoid contents were recorded in L. cercinense and myricitrin, myricetin, myricetin 3-O-β-D-galatopyranoside, luteolin 7-O-glucoside and rutin were the main phenolics in both species.
34482786	8	4	theme	luteolin	1089:1096	arg1	7-O-glucoside					1098:1110	luteolin 7-O-glucoside	1089:1110	luteolin 7-O-glucoside	1089:1110	High total phenolic and flavonoid contents were recorded in L. cercinense and myricitrin, myricetin, myricetin 3-O-β-D-galatopyranoside, luteolin 7-O-glucoside and rutin were the main phenolics in both species.
34482786	8	4	theme	luteolin	1089:1096	arg1	rutin					1116:1120	rutin	1116:1120	rutin	1116:1120	High total phenolic and flavonoid contents were recorded in L. cercinense and myricitrin, myricetin, myricetin 3-O-β-D-galatopyranoside, luteolin 7-O-glucoside and rutin were the main phenolics in both species.
34482786	9	5	theme	valuable	1225:1232	arg1	boitardii					1194:1202	L. boitardii	1191:1202	L. boitardii	1191:1202	The results obtained render L. boitardii and L. cercinense as valuable new natural sources for cosmetic and pharmacological applications.
34482786	9	5	theme	valuable	1225:1232	arg1	sources					1246:1252	valuable new natural sources	1225:1252	valuable new natural sources for cosmetic and pharmacological applications	1225:1298	The results obtained render L. boitardii and L. cercinense as valuable new natural sources for cosmetic and pharmacological applications.
34482786	9	5	theme	valuable	1225:1232	arg1	cercinense					1211:1220	L. cercinense	1208:1220	L. cercinense	1208:1220	The results obtained render L. boitardii and L. cercinense as valuable new natural sources for cosmetic and pharmacological applications.
34482786	4	6	theme	antioxidant	579:589	arg1	activities					591:600	total antioxidant activities	573:600	total antioxidant activities	573:600	Total phenolic and flavonoid contents, antioxidant activity (antiradical, reducing and total antioxidant activities), tyrosinase and α-glucosidase, collagenase inhibition activities were determined.
34482786	8	7	theme	High	952:955	arg1	total					957:961	High total phenolic	952:970	High total phenolic	952:970	High total phenolic and flavonoid contents were recorded in L. cercinense and myricitrin, myricetin, myricetin 3-O-β-D-galatopyranoside, luteolin 7-O-glucoside and rutin were the main phenolics in both species.
34482786	7	8	dep	cercinense	940:949	arg1	L.					937:938	L.	937:938	L.	937:938	In the tyrosinase and α-glucosidase inhibitor activity tests, the two species were highly active, especially L. cercinense.
34482786	4	9	theme	total	573:577	arg1	activities					591:600	total antioxidant activities	573:600	total antioxidant activities	573:600	Total phenolic and flavonoid contents, antioxidant activity (antiradical, reducing and total antioxidant activities), tyrosinase and α-glucosidase, collagenase inhibition activities were determined.
34482786	9	10	theme	natural	1238:1244	arg1	boitardii					1194:1202	L. boitardii	1191:1202	L. boitardii	1191:1202	The results obtained render L. boitardii and L. cercinense as valuable new natural sources for cosmetic and pharmacological applications.
34482786	9	10	theme	natural	1238:1244	arg1	sources					1246:1252	valuable new natural sources	1225:1252	valuable new natural sources for cosmetic and pharmacological applications	1225:1298	The results obtained render L. boitardii and L. cercinense as valuable new natural sources for cosmetic and pharmacological applications.
34482786	9	10	theme	natural	1238:1244	arg1	cercinense					1211:1220	L. cercinense	1208:1220	L. cercinense	1208:1220	The results obtained render L. boitardii and L. cercinense as valuable new natural sources for cosmetic and pharmacological applications.
34482786	7	11	from	active	918:923	arg1	tests					883:887	the tyrosinase and α-glucosidase inhibitor activity tests	831:887	the tyrosinase and α-glucosidase inhibitor activity tests	831:887	In the tyrosinase and α-glucosidase inhibitor activity tests, the two species were highly active, especially L. cercinense.
34482786	1	12	theme	Limonium	154:161	arg1	genus					163:167	Limonium genus	154:167	Limonium genus	154:167	Limonium genus is traditionally used in North Africa for disease treatment and in cosmetic.
34482786	8	13	from	phenolics	1136:1144	arg1	species					1154:1160	both species	1149:1160	both species	1149:1160	High total phenolic and flavonoid contents were recorded in L. cercinense and myricitrin, myricetin, myricetin 3-O-β-D-galatopyranoside, luteolin 7-O-glucoside and rutin were the main phenolics in both species.
34482786	2	14	theme	plants	381:386	arg1	activities					346:355	the antioxidant, anti-inflammatory and enzyme inhibitory activities	289:355	the antioxidant, anti-inflammatory and enzyme inhibitory activities of two Tunisian endemic plants, L. boitardii and L. cercinense	289:418	This study investigates for the first time the antioxidant, anti-inflammatory and enzyme inhibitory activities of two Tunisian endemic plants, L. boitardii and L. cercinense.
34482786	4	15	dep	activity	537:544	arg1	reducing					560:567	reducing	560:567	reducing	560:567	Total phenolic and flavonoid contents, antioxidant activity (antiradical, reducing and total antioxidant activities), tyrosinase and α-glucosidase, collagenase inhibition activities were determined.
34482786	4	15	dep	activity	537:544	arg1	antiradical					547:557	antiradical	547:557	antiradical	547:557	Total phenolic and flavonoid contents, antioxidant activity (antiradical, reducing and total antioxidant activities), tyrosinase and α-glucosidase, collagenase inhibition activities were determined.
34482786	4	15	dep	activity	537:544	arg1	activities					591:600	total antioxidant activities	573:600	total antioxidant activities	573:600	Total phenolic and flavonoid contents, antioxidant activity (antiradical, reducing and total antioxidant activities), tyrosinase and α-glucosidase, collagenase inhibition activities were determined.
34482786	2	16	theme	antioxidant	293:303	arg1	activities					346:355	the antioxidant, anti-inflammatory and enzyme inhibitory activities	289:355	the antioxidant, anti-inflammatory and enzyme inhibitory activities of two Tunisian endemic plants, L. boitardii and L. cercinense	289:418	This study investigates for the first time the antioxidant, anti-inflammatory and enzyme inhibitory activities of two Tunisian endemic plants, L. boitardii and L. cercinense.
34482786	8	17	theme	main	1131:1134	arg1	3-O-β-D-galatopyranoside					1063:1086	myricetin 3-O-β-D-galatopyranoside	1053:1086	myricetin 3-O-β-D-galatopyranoside	1053:1086	High total phenolic and flavonoid contents were recorded in L. cercinense and myricitrin, myricetin, myricetin 3-O-β-D-galatopyranoside, luteolin 7-O-glucoside and rutin were the main phenolics in both species.
34482786	8	17	theme	main	1131:1134	arg1	myricitrin					1030:1039	myricitrin	1030:1039	myricitrin	1030:1039	High total phenolic and flavonoid contents were recorded in L. cercinense and myricitrin, myricetin, myricetin 3-O-β-D-galatopyranoside, luteolin 7-O-glucoside and rutin were the main phenolics in both species.
34482786	8	17	theme	main	1131:1134	arg1	phenolics					1136:1144	the main phenolics	1127:1144	the main phenolics in both species	1127:1160	High total phenolic and flavonoid contents were recorded in L. cercinense and myricitrin, myricetin, myricetin 3-O-β-D-galatopyranoside, luteolin 7-O-glucoside and rutin were the main phenolics in both species.
34482786	8	17	theme	main	1131:1134	arg1	myricetin					1042:1050	myricetin	1042:1050	myricetin	1042:1050	High total phenolic and flavonoid contents were recorded in L. cercinense and myricitrin, myricetin, myricetin 3-O-β-D-galatopyranoside, luteolin 7-O-glucoside and rutin were the main phenolics in both species.
34482786	8	17	theme	main	1131:1134	arg1	7-O-glucoside					1098:1110	luteolin 7-O-glucoside	1089:1110	luteolin 7-O-glucoside	1089:1110	High total phenolic and flavonoid contents were recorded in L. cercinense and myricitrin, myricetin, myricetin 3-O-β-D-galatopyranoside, luteolin 7-O-glucoside and rutin were the main phenolics in both species.
34482786	8	17	theme	main	1131:1134	arg1	rutin					1116:1120	rutin	1116:1120	rutin	1116:1120	High total phenolic and flavonoid contents were recorded in L. cercinense and myricitrin, myricetin, myricetin 3-O-β-D-galatopyranoside, luteolin 7-O-glucoside and rutin were the main phenolics in both species.
34482786	2	18	theme	endemic	373:379	arg1	plants					381:386	two Tunisian endemic plants	360:386	two Tunisian endemic plants	360:386	This study investigates for the first time the antioxidant, anti-inflammatory and enzyme inhibitory activities of two Tunisian endemic plants, L. boitardii and L. cercinense.
34482786	2	18	theme	endemic	373:379	arg1	boitardii					392:400	L. boitardii	389:400	L. boitardii	389:400	This study investigates for the first time the antioxidant, anti-inflammatory and enzyme inhibitory activities of two Tunisian endemic plants, L. boitardii and L. cercinense.
34482786	2	18	theme	endemic	373:379	arg1	cercinense					409:418	L. cercinense	406:418	L. cercinense	406:418	This study investigates for the first time the antioxidant, anti-inflammatory and enzyme inhibitory activities of two Tunisian endemic plants, L. boitardii and L. cercinense.
34482786	5	19	theme	LPS-stimulated	713:726	arg1	macrophages					728:738	LPS-stimulated macrophages	713:738	LPS-stimulated macrophages	713:738	Inhibiting of NO release in LPS-stimulated macrophages was assessed.
34482786	7	20	theme	tyrosinase	835:844	arg1	tests					883:887	the tyrosinase and α-glucosidase inhibitor activity tests	831:887	the tyrosinase and α-glucosidase inhibitor activity tests	831:887	In the tyrosinase and α-glucosidase inhibitor activity tests, the two species were highly active, especially L. cercinense.
34482786	6	21	theme	anti-inflammatory	801:817	arg1	effects					819:825	strong antioxidant and anti-inflammatory effects	778:825	strong antioxidant and anti-inflammatory effects	778:825	L. cercinense exhibited strong antioxidant and anti-inflammatory effects.
34482786	7	22	theme	activity	874:881	arg1	tests					883:887	the tyrosinase and α-glucosidase inhibitor activity tests	831:887	the tyrosinase and α-glucosidase inhibitor activity tests	831:887	In the tyrosinase and α-glucosidase inhibitor activity tests, the two species were highly active, especially L. cercinense.
34482786	2	23	theme	Tunisian	364:371	arg1	plants					381:386	two Tunisian endemic plants	360:386	two Tunisian endemic plants	360:386	This study investigates for the first time the antioxidant, anti-inflammatory and enzyme inhibitory activities of two Tunisian endemic plants, L. boitardii and L. cercinense.
34482786	2	23	theme	Tunisian	364:371	arg1	boitardii					392:400	L. boitardii	389:400	L. boitardii	389:400	This study investigates for the first time the antioxidant, anti-inflammatory and enzyme inhibitory activities of two Tunisian endemic plants, L. boitardii and L. cercinense.
34482786	2	23	theme	Tunisian	364:371	arg1	cercinense					409:418	L. cercinense	406:418	L. cercinense	406:418	This study investigates for the first time the antioxidant, anti-inflammatory and enzyme inhibitory activities of two Tunisian endemic plants, L. boitardii and L. cercinense.
34482786	9	24	theme	new	1234:1236	arg1	boitardii					1194:1202	L. boitardii	1191:1202	L. boitardii	1191:1202	The results obtained render L. boitardii and L. cercinense as valuable new natural sources for cosmetic and pharmacological applications.
34482786	9	24	theme	new	1234:1236	arg1	sources					1246:1252	valuable new natural sources	1225:1252	valuable new natural sources for cosmetic and pharmacological applications	1225:1298	The results obtained render L. boitardii and L. cercinense as valuable new natural sources for cosmetic and pharmacological applications.
34482786	9	24	theme	new	1234:1236	arg1	cercinense					1211:1220	L. cercinense	1208:1220	L. cercinense	1208:1220	The results obtained render L. boitardii and L. cercinense as valuable new natural sources for cosmetic and pharmacological applications.
34482786	9	25	theme	cosmetic	1258:1265	arg1	applications					1287:1298	cosmetic and pharmacological applications	1258:1298	cosmetic and pharmacological applications	1258:1298	The results obtained render L. boitardii and L. cercinense as valuable new natural sources for cosmetic and pharmacological applications.
34482786	5	26	theme	release	702:708	arg1	Inhibiting					685:694	Inhibiting	685:694	Inhibiting of NO release in LPS-stimulated macrophages	685:738	Inhibiting of NO release in LPS-stimulated macrophages was assessed.
34482786	7	27	from	tests	883:887	arg1	active					918:923	active	918:923	active	918:923	In the tyrosinase and α-glucosidase inhibitor activity tests, the two species were highly active, especially L. cercinense.
34482786	7	27	from	tests	883:887	arg1	species					898:904	the two species	890:904	the two species	890:904	In the tyrosinase and α-glucosidase inhibitor activity tests, the two species were highly active, especially L. cercinense.
34482786	4	28	theme	α-glucosidase	619:631	arg1	activities					657:666	tyrosinase and α-glucosidase, collagenase inhibition activities	604:666	tyrosinase and α-glucosidase, collagenase inhibition activities	604:666	Total phenolic and flavonoid contents, antioxidant activity (antiradical, reducing and total antioxidant activities), tyrosinase and α-glucosidase, collagenase inhibition activities were determined.
34482786	4	28	theme	α-glucosidase	619:631	arg1	phenolic					492:499	phenolic	492:499	phenolic	492:499	Total phenolic and flavonoid contents, antioxidant activity (antiradical, reducing and total antioxidant activities), tyrosinase and α-glucosidase, collagenase inhibition activities were determined.
34482786	0	29	theme	phenolic	18:25	arg1	composition					27:37	phenolic composition	18:37	phenolic composition	18:37	Bioactivities and phenolic composition of Limonium boitardii Maire and L. cercinense Brullo & Erben (Plumbaginaceae): two Tunisian strict endemic plants.
34482786	9	30	theme	pharmacological	1271:1285	arg1	applications					1287:1298	cosmetic and pharmacological applications	1258:1298	cosmetic and pharmacological applications	1258:1298	The results obtained render L. boitardii and L. cercinense as valuable new natural sources for cosmetic and pharmacological applications.
34482786	2	31	theme	first	278:282	arg1	time					284:287	the first time	274:287	the first time	274:287	This study investigates for the first time the antioxidant, anti-inflammatory and enzyme inhibitory activities of two Tunisian endemic plants, L. boitardii and L. cercinense.
34482786	7	32	theme	α-glucosidase	850:862	arg1	activity					874:881	α-glucosidase inhibitor activity	850:881	α-glucosidase inhibitor activity	850:881	In the tyrosinase and α-glucosidase inhibitor activity tests, the two species were highly active, especially L. cercinense.
34482786	0	33	theme	Tunisian	122:129	arg1	plants					146:151	two Tunisian strict endemic plants	118:151	Bioactivities and phenolic composition of Limonium boitardii Maire and L. cercinense Brullo & Erben (Plumbaginaceae): two Tunisian strict endemic plants.	0:152	Bioactivities and phenolic composition of Limonium boitardii Maire and L. cercinense Brullo & Erben (Plumbaginaceae): two Tunisian strict endemic plants.
34482786	3	34	theme	phenolic	433:440	arg1	compounds					442:450	phenolic compounds	433:450	phenolic compounds	433:450	Analysis of phenolic compounds was carried out by using RP-HPLC.
34482786	7	35	theme	inhibitor	864:872	arg1	activity					874:881	α-glucosidase inhibitor activity	850:881	α-glucosidase inhibitor activity	850:881	In the tyrosinase and α-glucosidase inhibitor activity tests, the two species were highly active, especially L. cercinense.
34482786	4	36	theme	collagenase	634:644	arg1	α-glucosidase					619:631	α-glucosidase	619:631	α-glucosidase	619:631	Total phenolic and flavonoid contents, antioxidant activity (antiradical, reducing and total antioxidant activities), tyrosinase and α-glucosidase, collagenase inhibition activities were determined.
34482786	4	36	theme	collagenase	634:644	arg1	inhibition					646:655	collagenase inhibition	634:655	collagenase inhibition	634:655	Total phenolic and flavonoid contents, antioxidant activity (antiradical, reducing and total antioxidant activities), tyrosinase and α-glucosidase, collagenase inhibition activities were determined.
34482786	6	37	theme	antioxidant	785:795	arg1	effects					819:825	strong antioxidant and anti-inflammatory effects	778:825	strong antioxidant and anti-inflammatory effects	778:825	L. cercinense exhibited strong antioxidant and anti-inflammatory effects.
34482786	3	38	theme	compounds	442:450	arg1	Analysis					421:428	Analysis	421:428	Analysis of phenolic compounds	421:450	Analysis of phenolic compounds was carried out by using RP-HPLC.
34482786	0	39	theme	Maire	61:65	arg1	Bioactivities					0:12	Bioactivities	0:12	Bioactivities	0:12	Bioactivities and phenolic composition of Limonium boitardii Maire and L. cercinense Brullo & Erben (Plumbaginaceae): two Tunisian strict endemic plants.
34482786	0	39	theme	Maire	61:65	arg1	composition					27:37	phenolic composition	18:37	phenolic composition	18:37	Bioactivities and phenolic composition of Limonium boitardii Maire and L. cercinense Brullo & Erben (Plumbaginaceae): two Tunisian strict endemic plants.
34482786	4	40	theme	flavonoid	505:513	arg1	contents					515:522	Total phenolic and flavonoid contents	486:522	contents	515:522	Total phenolic and flavonoid contents, antioxidant activity (antiradical, reducing and total antioxidant activities), tyrosinase and α-glucosidase, collagenase inhibition activities were determined.
34482786	0	41	theme	endemic	138:144	arg1	plants					146:151	two Tunisian strict endemic plants	118:151	Bioactivities and phenolic composition of Limonium boitardii Maire and L. cercinense Brullo & Erben (Plumbaginaceae): two Tunisian strict endemic plants.	0:152	Bioactivities and phenolic composition of Limonium boitardii Maire and L. cercinense Brullo & Erben (Plumbaginaceae): two Tunisian strict endemic plants.
34482786	6	42	theme	strong	778:783	arg1	effects					819:825	strong antioxidant and anti-inflammatory effects	778:825	strong antioxidant and anti-inflammatory effects	778:825	L. cercinense exhibited strong antioxidant and anti-inflammatory effects.
34482786	4	43	theme	antioxidant	525:535	arg1	phenolic					492:499	phenolic	492:499	phenolic	492:499	Total phenolic and flavonoid contents, antioxidant activity (antiradical, reducing and total antioxidant activities), tyrosinase and α-glucosidase, collagenase inhibition activities were determined.
34482786	4	43	theme	antioxidant	525:535	arg1	activity					537:544	antioxidant activity	525:544	antioxidant activity (antiradical, reducing and total antioxidant activities)	525:601	Total phenolic and flavonoid contents, antioxidant activity (antiradical, reducing and total antioxidant activities), tyrosinase and α-glucosidase, collagenase inhibition activities were determined.
34482786	0	44	theme	strict	131:136	arg1	plants					146:151	two Tunisian strict endemic plants	118:151	Bioactivities and phenolic composition of Limonium boitardii Maire and L. cercinense Brullo & Erben (Plumbaginaceae): two Tunisian strict endemic plants.	0:152	Bioactivities and phenolic composition of Limonium boitardii Maire and L. cercinense Brullo & Erben (Plumbaginaceae): two Tunisian strict endemic plants.
34482786	1	45	used	used	186:189	arg2	genus					163:167	Limonium genus	154:167	Limonium genus	154:167	Limonium genus is traditionally used in North Africa for disease treatment and in cosmetic.
34482786	8	46	theme	phenolic	963:970	arg1	total					957:961	High total phenolic	952:970	High total phenolic	952:970	High total phenolic and flavonoid contents were recorded in L. cercinense and myricitrin, myricetin, myricetin 3-O-β-D-galatopyranoside, luteolin 7-O-glucoside and rutin were the main phenolics in both species.
34482786	2	47	theme	anti-inflammatory	306:322	arg1	activities					346:355	the antioxidant, anti-inflammatory and enzyme inhibitory activities	289:355	the antioxidant, anti-inflammatory and enzyme inhibitory activities of two Tunisian endemic plants, L. boitardii and L. cercinense	289:418	This study investigates for the first time the antioxidant, anti-inflammatory and enzyme inhibitory activities of two Tunisian endemic plants, L. boitardii and L. cercinense.
34482786	5	48	theme	NO	699:700	arg1	release					702:708	NO release	699:708	NO release	699:708	Inhibiting of NO release in LPS-stimulated macrophages was assessed.
34482786	6	49	theme	L.	754:755	arg1	cercinense					757:766	L. cercinense	754:766	L. cercinense	754:766	L. cercinense exhibited strong antioxidant and anti-inflammatory effects.
34482786	1	50	theme	disease	211:217	arg1	treatment					219:227	disease treatment	211:227	disease treatment	211:227	Limonium genus is traditionally used in North Africa for disease treatment and in cosmetic.
34482786	8	51	theme	flavonoid	976:984	arg1	contents					986:993	flavonoid contents	976:993	flavonoid contents	976:993	High total phenolic and flavonoid contents were recorded in L. cercinense and myricitrin, myricetin, myricetin 3-O-β-D-galatopyranoside, luteolin 7-O-glucoside and rutin were the main phenolics in both species.
34482786	4	52	theme	tyrosinase	604:613	arg1	activities					657:666	tyrosinase and α-glucosidase, collagenase inhibition activities	604:666	tyrosinase and α-glucosidase, collagenase inhibition activities	604:666	Total phenolic and flavonoid contents, antioxidant activity (antiradical, reducing and total antioxidant activities), tyrosinase and α-glucosidase, collagenase inhibition activities were determined.
34482786	4	52	theme	tyrosinase	604:613	arg1	phenolic					492:499	phenolic	492:499	phenolic	492:499	Total phenolic and flavonoid contents, antioxidant activity (antiradical, reducing and total antioxidant activities), tyrosinase and α-glucosidase, collagenase inhibition activities were determined.
34482786	0	53	dep	Bioactivities	0:12	arg1	plants					146:151	two Tunisian strict endemic plants	118:151	Bioactivities and phenolic composition of Limonium boitardii Maire and L. cercinense Brullo & Erben (Plumbaginaceae): two Tunisian strict endemic plants.	0:152	Bioactivities and phenolic composition of Limonium boitardii Maire and L. cercinense Brullo & Erben (Plumbaginaceae): two Tunisian strict endemic plants.
34482786	5	54	from	Inhibiting	685:694	arg1	macrophages					728:738	LPS-stimulated macrophages	713:738	LPS-stimulated macrophages	713:738	Inhibiting of NO release in LPS-stimulated macrophages was assessed.
32829136	7	0	theme	causes	1484:1489	arg1	one					1473:1475	one	1473:1475	one	1473:1475	The differences in production of amino acids by D75 and D76 strains grown together and separately were found and concluded to be a consequence and/or one of the causes of synergism and syntropy of the strains.
32829136	7	0	theme	causes	1484:1489	arg1	causes					1484:1489	the causes	1480:1489	the causes of synergism and syntropy of the strains	1480:1530	The differences in production of amino acids by D75 and D76 strains grown together and separately were found and concluded to be a consequence and/or one of the causes of synergism and syntropy of the strains.
32829136	4	1	theme	sodium	898:903	arg1	acetate					905:911	sodium acetate	898:911	sodium acetate	898:911	The effect of CDs, Cu2+, sodium acetate, β-CDs concentrations and pH of background electrolyte (BGE) on the electrophoretic mobilities of AAs was thoroughly investigated.
32829136	6	2	theme	developed	1183:1191	arg1	method					1193:1198	The developed method	1179:1198	The developed method	1179:1198	The developed method possessed high analysis-to-analysis and day-to-day repeatability of migration times (RSD ≤ 1.0% and ≤ 2.5%, respectively).
32829136	3	3	theme	derivates	754:762	arg1	application					687:697	proposed application	678:697	proposed application of β-cyclodextrin (β-CD) and its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD) as pseudo stationary phases	678:831	Native amino acids and lactic acid were separated and detected as complexes with Cu2+ ions, while proposed application of β-cyclodextrin (β-CD) and its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD) as pseudo stationary phases provided better separation selectivity.
32829136	6	4	theme	%	1294:1294	arg1	%					1305:1305	RSD ≤ 1.0% and ≤ 2.5%	1285:1305	RSD ≤ 1.0% and ≤ 2.5%	1285:1305	The developed method possessed high analysis-to-analysis and day-to-day repeatability of migration times (RSD ≤ 1.0% and ≤ 2.5%, respectively).
32829136	2	5	with	UV-detection	445:456	arg1	preparation					497:507	minimum sample preparation	482:507	minimum sample preparation	482:507	The method provided sensitive UV-detection of native analytes with minimum sample preparation and appeared to be extremely useful for the analysis of culture media.
32829136	4	6	theme	CDs	887:889	arg1	effect					877:882	The effect	873:882	The effect of CDs, Cu2+, sodium acetate, β-CDs concentrations and pH of background electrolyte (BGE) on the electrophoretic mobilities of AAs	873:1013	The effect of CDs, Cu2+, sodium acetate, β-CDs concentrations and pH of background electrolyte (BGE) on the electrophoretic mobilities of AAs was thoroughly investigated.
32829136	3	7	theme	Native	580:585	arg1	acids					593:597	Native amino acids	580:597	Native amino acids	580:597	Native amino acids and lactic acid were separated and detected as complexes with Cu2+ ions, while proposed application of β-cyclodextrin (β-CD) and its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD) as pseudo stationary phases provided better separation selectivity.
32829136	3	7	theme	Native	580:585	arg1	complexes					646:654	complexes	646:654	complexes with Cu2+ ions	646:669	Native amino acids and lactic acid were separated and detected as complexes with Cu2+ ions, while proposed application of β-cyclodextrin (β-CD) and its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD) as pseudo stationary phases provided better separation selectivity.
32829136	1	8	theme	selective	204:212	arg1	determination					227:239	selective simultaneous determination	204:239	selective simultaneous determination	204:239	A capillary electrophoresis method for selective simultaneous determination and quantitation of native amino acids and lactic acid during cultivation of Lactobacillus helveticus D75 and D76 strains on the MRS-1 and milk nutrient media was presented.
32829136	4	9	theme	Cu2+	892:895	arg1	effect					877:882	The effect	873:882	The effect of CDs, Cu2+, sodium acetate, β-CDs concentrations and pH of background electrolyte (BGE) on the electrophoretic mobilities of AAs	873:1013	The effect of CDs, Cu2+, sodium acetate, β-CDs concentrations and pH of background electrolyte (BGE) on the electrophoretic mobilities of AAs was thoroughly investigated.
32829136	6	10	theme	migration	1268:1276	arg1	times					1278:1282	migration times	1268:1282	migration times (RSD ≤ 1.0% and ≤ 2.5%, respectively)	1268:1320	The developed method possessed high analysis-to-analysis and day-to-day repeatability of migration times (RSD ≤ 1.0% and ≤ 2.5%, respectively).
32829136	4	11	theme	background	945:954	arg1	BGE					969:971	BGE	969:971	BGE	969:971	The effect of CDs, Cu2+, sodium acetate, β-CDs concentrations and pH of background electrolyte (BGE) on the electrophoretic mobilities of AAs was thoroughly investigated.
32829136	4	11	theme	background	945:954	arg1	electrolyte					956:966	background electrolyte	945:966	background electrolyte (BGE)	945:972	The effect of CDs, Cu2+, sodium acetate, β-CDs concentrations and pH of background electrolyte (BGE) on the electrophoretic mobilities of AAs was thoroughly investigated.
32829136	3	12	theme	sulfated	765:772	arg1	derivates					754:762	its charged and uncharged derivates	728:762	its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD)	728:803	Native amino acids and lactic acid were separated and detected as complexes with Cu2+ ions, while proposed application of β-cyclodextrin (β-CD) and its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD) as pseudo stationary phases provided better separation selectivity.
32829136	3	12	theme	sulfated	765:772	arg1	β-CD					774:777	sulfated β-CD	765:777	sulfated β-CD	765:777	Native amino acids and lactic acid were separated and detected as complexes with Cu2+ ions, while proposed application of β-cyclodextrin (β-CD) and its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD) as pseudo stationary phases provided better separation selectivity.
32829136	3	13	theme	lactic	603:608	arg1	acid					610:613	lactic acid	603:613	lactic acid	603:613	Native amino acids and lactic acid were separated and detected as complexes with Cu2+ ions, while proposed application of β-cyclodextrin (β-CD) and its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD) as pseudo stationary phases provided better separation selectivity.
32829136	3	14	theme	pseudo	808:813	arg1	phases					826:831	pseudo stationary phases	808:831	pseudo stationary phases	808:831	Native amino acids and lactic acid were separated and detected as complexes with Cu2+ ions, while proposed application of β-cyclodextrin (β-CD) and its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD) as pseudo stationary phases provided better separation selectivity.
32829136	4	15	from	effect	877:882	arg1	mobilities					997:1006	the electrophoretic mobilities	977:1006	the electrophoretic mobilities of AAs	977:1013	The effect of CDs, Cu2+, sodium acetate, β-CDs concentrations and pH of background electrolyte (BGE) on the electrophoretic mobilities of AAs was thoroughly investigated.
32829136	1	16	theme	native	261:266	arg1	acids					274:278	native amino acids	261:278	native amino acids	261:278	A capillary electrophoresis method for selective simultaneous determination and quantitation of native amino acids and lactic acid during cultivation of Lactobacillus helveticus D75 and D76 strains on the MRS-1 and milk nutrient media was presented.
32829136	4	17	theme	concentrations	920:933	arg1	effect					877:882	The effect	873:882	The effect of CDs, Cu2+, sodium acetate, β-CDs concentrations and pH of background electrolyte (BGE) on the electrophoretic mobilities of AAs	873:1013	The effect of CDs, Cu2+, sodium acetate, β-CDs concentrations and pH of background electrolyte (BGE) on the electrophoretic mobilities of AAs was thoroughly investigated.
32829136	6	18	theme	day-to-day	1240:1249	arg1	repeatability					1251:1263	day-to-day repeatability	1240:1263	day-to-day repeatability	1240:1263	The developed method possessed high analysis-to-analysis and day-to-day repeatability of migration times (RSD ≤ 1.0% and ≤ 2.5%, respectively).
32829136	5	19	theme	BGE	1067:1069	arg1	composition					1048:1058	The composition	1044:1058	The composition of the BGE	1044:1069	The composition of the BGE was found to be as follows: 20 mM acetate buffer solution, 50 mM CuSO4, 10 mM 2-hydroxypropyl-β-CD, pH 4.3.
32829136	5	20	theme	10 mM	1143:1147	arg1	follows					1090:1096	follows	1090:1096	follows	1090:1096	The composition of the BGE was found to be as follows: 20 mM acetate buffer solution, 50 mM CuSO4, 10 mM 2-hydroxypropyl-β-CD, pH 4.3.
32829136	5	20	theme	10 mM	1143:1147	arg1	2-hydroxypropyl-β-CD					1149:1168	10 mM 2-hydroxypropyl-β-CD	1143:1168	10 mM 2-hydroxypropyl-β-CD	1143:1168	The composition of the BGE was found to be as follows: 20 mM acetate buffer solution, 50 mM CuSO4, 10 mM 2-hydroxypropyl-β-CD, pH 4.3.
32829136	4	21	theme	pH	939:940	arg1	effect					877:882	The effect	873:882	The effect of CDs, Cu2+, sodium acetate, β-CDs concentrations and pH of background electrolyte (BGE) on the electrophoretic mobilities of AAs	873:1013	The effect of CDs, Cu2+, sodium acetate, β-CDs concentrations and pH of background electrolyte (BGE) on the electrophoretic mobilities of AAs was thoroughly investigated.
32829136	3	22	theme	better	842:847	arg1	selectivity					860:870	better separation selectivity	842:870	better separation selectivity	842:870	Native amino acids and lactic acid were separated and detected as complexes with Cu2+ ions, while proposed application of β-cyclodextrin (β-CD) and its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD) as pseudo stationary phases provided better separation selectivity.
32829136	7	23	theme	amino	1356:1360	arg1	acids					1362:1366	amino acids	1356:1366	amino acids	1356:1366	The differences in production of amino acids by D75 and D76 strains grown together and separately were found and concluded to be a consequence and/or one of the causes of synergism and syntropy of the strains.
32829136	0	24	theme	inLactobacillus	52:66	arg1	media					86:90	inLactobacillus helveticusculture media	52:90	inLactobacillus helveticusculture media	52:90	Determination of native amino acids and lactic acid inLactobacillus helveticusculture media by capillary electrophoresis using Cu2+and β-cyclodextrins as additives.
32829136	1	25	theme	capillary	167:175	arg1	electrophoresis					177:191	A capillary electrophoresis	165:191	A capillary electrophoresis method for selective simultaneous determination and quantitation of native amino acids and lactic acid during cultivation of Lactobacillus helveticus D75 and D76 strains on the MRS-1 and milk nutrient media	165:398	A capillary electrophoresis method for selective simultaneous determination and quantitation of native amino acids and lactic acid during cultivation of Lactobacillus helveticus D75 and D76 strains on the MRS-1 and milk nutrient media was presented.
32829136	4	26	theme	AAs	1011:1013	arg1	mobilities					997:1006	the electrophoretic mobilities	977:1006	the electrophoretic mobilities of AAs	977:1013	The effect of CDs, Cu2+, sodium acetate, β-CDs concentrations and pH of background electrolyte (BGE) on the electrophoretic mobilities of AAs was thoroughly investigated.
32829136	1	27	theme	milk	380:383	arg1	media					394:398	the MRS-1 and milk nutrient media	366:398	media	394:398	A capillary electrophoresis method for selective simultaneous determination and quantitation of native amino acids and lactic acid during cultivation of Lactobacillus helveticus D75 and D76 strains on the MRS-1 and milk nutrient media was presented.
32829136	2	28	theme	media	573:577	arg1	analysis					553:560	the analysis	549:560	the analysis of culture media	549:577	The method provided sensitive UV-detection of native analytes with minimum sample preparation and appeared to be extremely useful for the analysis of culture media.
32829136	7	29	theme	D75	1371:1373	arg1	strains					1383:1389	D75 and D76 strains	1371:1389	D75 and D76 strains grown together and separately	1371:1419	The differences in production of amino acids by D75 and D76 strains grown together and separately were found and concluded to be a consequence and/or one of the causes of synergism and syntropy of the strains.
32829136	0	30	theme	capillary	95:103	arg1	electrophoresis					105:119	capillary electrophoresis	95:119	capillary electrophoresis	95:119	Determination of native amino acids and lactic acid inLactobacillus helveticusculture media by capillary electrophoresis using Cu2+and β-cyclodextrins as additives.
32829136	5	31	theme	20 mM	1099:1103	arg1	acetate					1105:1111	acetate	1105:1111	acetate	1105:1111	The composition of the BGE was found to be as follows: 20 mM acetate buffer solution, 50 mM CuSO4, 10 mM 2-hydroxypropyl-β-CD, pH 4.3.
32829136	2	32	theme	sensitive	435:443	arg1	UV-detection					445:456	sensitive UV-detection	435:456	sensitive UV-detection of native analytes with minimum sample preparation	435:507	The method provided sensitive UV-detection of native analytes with minimum sample preparation and appeared to be extremely useful for the analysis of culture media.
32829136	5	33	theme	buffer	1113:1118	arg1	solution					1120:1127	20 mM acetate buffer solution	1099:1127	20 mM acetate buffer solution	1099:1127	The composition of the BGE was found to be as follows: 20 mM acetate buffer solution, 50 mM CuSO4, 10 mM 2-hydroxypropyl-β-CD, pH 4.3.
32829136	0	34	theme	helveticusculture	68:84	arg1	media					86:90	inLactobacillus helveticusculture media	52:90	inLactobacillus helveticusculture media	52:90	Determination of native amino acids and lactic acid inLactobacillus helveticusculture media by capillary electrophoresis using Cu2+and β-cyclodextrins as additives.
32829136	1	35	theme	Lactobacillus	318:330	arg1	helveticus					332:341	Lactobacillus helveticus D75	318:345	Lactobacillus helveticus D75	318:345	A capillary electrophoresis method for selective simultaneous determination and quantitation of native amino acids and lactic acid during cultivation of Lactobacillus helveticus D75 and D76 strains on the MRS-1 and milk nutrient media was presented.
32829136	2	36	theme	minimum	482:488	arg1	preparation					497:507	minimum sample preparation	482:507	minimum sample preparation	482:507	The method provided sensitive UV-detection of native analytes with minimum sample preparation and appeared to be extremely useful for the analysis of culture media.
32829136	2	37	theme	analytes	468:475	arg1	UV-detection					445:456	sensitive UV-detection	435:456	sensitive UV-detection of native analytes with minimum sample preparation	435:507	The method provided sensitive UV-detection of native analytes with minimum sample preparation and appeared to be extremely useful for the analysis of culture media.
32829136	0	38	theme	native	17:22	arg1	acids					30:34	native amino acids	17:34	native amino acids	17:34	Determination of native amino acids and lactic acid inLactobacillus helveticusculture media by capillary electrophoresis using Cu2+and β-cyclodextrins as additives.
32829136	7	39	theme	strains	1524:1530	arg1	syntropy					1508:1515	syntropy	1508:1515	syntropy of the strains	1508:1530	The differences in production of amino acids by D75 and D76 strains grown together and separately were found and concluded to be a consequence and/or one of the causes of synergism and syntropy of the strains.
32829136	7	39	theme	strains	1524:1530	arg1	synergism					1494:1502	synergism	1494:1502	synergism	1494:1502	The differences in production of amino acids by D75 and D76 strains grown together and separately were found and concluded to be a consequence and/or one of the causes of synergism and syntropy of the strains.
32829136	0	40	theme	acids	30:34	arg1	Determination					0:12	Determination	0:12	Determination of native amino acids and lactic acid inLactobacillus helveticusculture media by capillary electrophoresis	0:119	Determination of native amino acids and lactic acid inLactobacillus helveticusculture media by capillary electrophoresis using Cu2+and β-cyclodextrins as additives.
32829136	1	41	theme	D76	351:353	arg1	strains					355:361	D76 strains	351:361	D76 strains	351:361	A capillary electrophoresis method for selective simultaneous determination and quantitation of native amino acids and lactic acid during cultivation of Lactobacillus helveticus D75 and D76 strains on the MRS-1 and milk nutrient media was presented.
32829136	6	42	contain	possessed	1200:1208	arg1	method					1193:1198	The developed method	1179:1198	The developed method	1179:1198	The developed method possessed high analysis-to-analysis and day-to-day repeatability of migration times (RSD ≤ 1.0% and ≤ 2.5%, respectively).
32829136	6	42	contain	possessed	1200:1208	arg2	repeatability					1251:1263	day-to-day repeatability	1240:1263	day-to-day repeatability	1240:1263	The developed method possessed high analysis-to-analysis and day-to-day repeatability of migration times (RSD ≤ 1.0% and ≤ 2.5%, respectively).
32829136	6	42	contain	possessed	1200:1208	arg2	analysis-to-analysis					1215:1234	high analysis-to-analysis	1210:1234	high analysis-to-analysis	1210:1234	The developed method possessed high analysis-to-analysis and day-to-day repeatability of migration times (RSD ≤ 1.0% and ≤ 2.5%, respectively).
32829136	0	43	theme	lactic	40:45	arg1	acid					47:50	lactic acid	40:50	lactic acid	40:50	Determination of native amino acids and lactic acid inLactobacillus helveticusculture media by capillary electrophoresis using Cu2+and β-cyclodextrins as additives.
32829136	3	44	dep	derivates	754:762	arg1	2-hydroxypropyl-β-CD					783:802	2-hydroxypropyl-β-CD	783:802	2-hydroxypropyl-β-CD	783:802	Native amino acids and lactic acid were separated and detected as complexes with Cu2+ ions, while proposed application of β-cyclodextrin (β-CD) and its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD) as pseudo stationary phases provided better separation selectivity.
32829136	3	44	dep	derivates	754:762	arg1	derivates					754:762	its charged and uncharged derivates	728:762	its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD)	728:803	Native amino acids and lactic acid were separated and detected as complexes with Cu2+ ions, while proposed application of β-cyclodextrin (β-CD) and its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD) as pseudo stationary phases provided better separation selectivity.
32829136	3	44	dep	derivates	754:762	arg1	β-CD					774:777	sulfated β-CD	765:777	sulfated β-CD	765:777	Native amino acids and lactic acid were separated and detected as complexes with Cu2+ ions, while proposed application of β-cyclodextrin (β-CD) and its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD) as pseudo stationary phases provided better separation selectivity.
32829136	0	45	dep	acids	30:34	arg1	media					86:90	inLactobacillus helveticusculture media	52:90	inLactobacillus helveticusculture media	52:90	Determination of native amino acids and lactic acid inLactobacillus helveticusculture media by capillary electrophoresis using Cu2+and β-cyclodextrins as additives.
32829136	1	46	theme	amino	268:272	arg1	acids					274:278	native amino acids	261:278	native amino acids	261:278	A capillary electrophoresis method for selective simultaneous determination and quantitation of native amino acids and lactic acid during cultivation of Lactobacillus helveticus D75 and D76 strains on the MRS-1 and milk nutrient media was presented.
32829136	7	47	from	differences	1327:1337	arg1	production					1342:1351	production	1342:1351	production of amino acids by D75 and D76 strains grown together and separately	1342:1419	The differences in production of amino acids by D75 and D76 strains grown together and separately were found and concluded to be a consequence and/or one of the causes of synergism and syntropy of the strains.
32829136	3	48	theme	β-cyclodextrin	702:715	arg1	application					687:697	proposed application	678:697	proposed application of β-cyclodextrin (β-CD) and its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD) as pseudo stationary phases	678:831	Native amino acids and lactic acid were separated and detected as complexes with Cu2+ ions, while proposed application of β-cyclodextrin (β-CD) and its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD) as pseudo stationary phases provided better separation selectivity.
32829136	3	49	theme	charged	732:738	arg1	2-hydroxypropyl-β-CD					783:802	2-hydroxypropyl-β-CD	783:802	2-hydroxypropyl-β-CD	783:802	Native amino acids and lactic acid were separated and detected as complexes with Cu2+ ions, while proposed application of β-cyclodextrin (β-CD) and its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD) as pseudo stationary phases provided better separation selectivity.
32829136	3	49	theme	charged	732:738	arg1	derivates					754:762	its charged and uncharged derivates	728:762	its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD)	728:803	Native amino acids and lactic acid were separated and detected as complexes with Cu2+ ions, while proposed application of β-cyclodextrin (β-CD) and its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD) as pseudo stationary phases provided better separation selectivity.
32829136	3	49	theme	charged	732:738	arg1	β-CD					774:777	sulfated β-CD	765:777	sulfated β-CD	765:777	Native amino acids and lactic acid were separated and detected as complexes with Cu2+ ions, while proposed application of β-cyclodextrin (β-CD) and its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD) as pseudo stationary phases provided better separation selectivity.
32829136	1	50	theme	acid	291:294	arg1	strains					355:361	D76 strains	351:361	D76 strains	351:361	A capillary electrophoresis method for selective simultaneous determination and quantitation of native amino acids and lactic acid during cultivation of Lactobacillus helveticus D75 and D76 strains on the MRS-1 and milk nutrient media was presented.
32829136	1	50	theme	acid	291:294	arg1	determination					227:239	selective simultaneous determination	204:239	selective simultaneous determination	204:239	A capillary electrophoresis method for selective simultaneous determination and quantitation of native amino acids and lactic acid during cultivation of Lactobacillus helveticus D75 and D76 strains on the MRS-1 and milk nutrient media was presented.
32829136	1	50	theme	acid	291:294	arg1	quantitation					245:256	quantitation	245:256	quantitation	245:256	A capillary electrophoresis method for selective simultaneous determination and quantitation of native amino acids and lactic acid during cultivation of Lactobacillus helveticus D75 and D76 strains on the MRS-1 and milk nutrient media was presented.
32829136	8	51	theme	media	1606:1610	arg1	analysis					1586:1593	the analysis	1582:1593	the analysis of culture media	1582:1610	The developed method proved to be applicable for the analysis of culture media.
32829136	3	52	theme	amino	587:591	arg1	acids					593:597	Native amino acids	580:597	Native amino acids	580:597	Native amino acids and lactic acid were separated and detected as complexes with Cu2+ ions, while proposed application of β-cyclodextrin (β-CD) and its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD) as pseudo stationary phases provided better separation selectivity.
32829136	3	52	theme	amino	587:591	arg1	complexes					646:654	complexes	646:654	complexes with Cu2+ ions	646:669	Native amino acids and lactic acid were separated and detected as complexes with Cu2+ ions, while proposed application of β-cyclodextrin (β-CD) and its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD) as pseudo stationary phases provided better separation selectivity.
32829136	4	53	theme	acetate	905:911	arg1	effect					877:882	The effect	873:882	The effect of CDs, Cu2+, sodium acetate, β-CDs concentrations and pH of background electrolyte (BGE) on the electrophoretic mobilities of AAs	873:1013	The effect of CDs, Cu2+, sodium acetate, β-CDs concentrations and pH of background electrolyte (BGE) on the electrophoretic mobilities of AAs was thoroughly investigated.
32829136	3	54	theme	uncharged	744:752	arg1	2-hydroxypropyl-β-CD					783:802	2-hydroxypropyl-β-CD	783:802	2-hydroxypropyl-β-CD	783:802	Native amino acids and lactic acid were separated and detected as complexes with Cu2+ ions, while proposed application of β-cyclodextrin (β-CD) and its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD) as pseudo stationary phases provided better separation selectivity.
32829136	3	54	theme	uncharged	744:752	arg1	derivates					754:762	its charged and uncharged derivates	728:762	its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD)	728:803	Native amino acids and lactic acid were separated and detected as complexes with Cu2+ ions, while proposed application of β-cyclodextrin (β-CD) and its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD) as pseudo stationary phases provided better separation selectivity.
32829136	3	54	theme	uncharged	744:752	arg1	β-CD					774:777	sulfated β-CD	765:777	sulfated β-CD	765:777	Native amino acids and lactic acid were separated and detected as complexes with Cu2+ ions, while proposed application of β-cyclodextrin (β-CD) and its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD) as pseudo stationary phases provided better separation selectivity.
32829136	6	55	theme	times	1278:1282	arg1	analysis-to-analysis					1215:1234	high analysis-to-analysis	1210:1234	high analysis-to-analysis	1210:1234	The developed method possessed high analysis-to-analysis and day-to-day repeatability of migration times (RSD ≤ 1.0% and ≤ 2.5%, respectively).
32829136	6	55	theme	times	1278:1282	arg1	repeatability					1251:1263	day-to-day repeatability	1240:1263	day-to-day repeatability	1240:1263	The developed method possessed high analysis-to-analysis and day-to-day repeatability of migration times (RSD ≤ 1.0% and ≤ 2.5%, respectively).
32829136	1	56	theme	simultaneous	214:225	arg1	determination					227:239	selective simultaneous determination	204:239	selective simultaneous determination	204:239	A capillary electrophoresis method for selective simultaneous determination and quantitation of native amino acids and lactic acid during cultivation of Lactobacillus helveticus D75 and D76 strains on the MRS-1 and milk nutrient media was presented.
32829136	4	57	theme	electrolyte	956:966	arg1	Cu2+					892:895	Cu2+	892:895	Cu2+	892:895	The effect of CDs, Cu2+, sodium acetate, β-CDs concentrations and pH of background electrolyte (BGE) on the electrophoretic mobilities of AAs was thoroughly investigated.
32829136	4	57	theme	electrolyte	956:966	arg1	pH					939:940	pH	939:940	pH	939:940	The effect of CDs, Cu2+, sodium acetate, β-CDs concentrations and pH of background electrolyte (BGE) on the electrophoretic mobilities of AAs was thoroughly investigated.
32829136	4	57	theme	electrolyte	956:966	arg1	acetate					905:911	sodium acetate	898:911	sodium acetate	898:911	The effect of CDs, Cu2+, sodium acetate, β-CDs concentrations and pH of background electrolyte (BGE) on the electrophoretic mobilities of AAs was thoroughly investigated.
32829136	4	57	theme	electrolyte	956:966	arg1	CDs					887:889	CDs	887:889	CDs	887:889	The effect of CDs, Cu2+, sodium acetate, β-CDs concentrations and pH of background electrolyte (BGE) on the electrophoretic mobilities of AAs was thoroughly investigated.
32829136	4	57	theme	electrolyte	956:966	arg1	concentrations					920:933	β-CDs concentrations	914:933	β-CDs concentrations	914:933	The effect of CDs, Cu2+, sodium acetate, β-CDs concentrations and pH of background electrolyte (BGE) on the electrophoretic mobilities of AAs was thoroughly investigated.
32829136	3	58	theme	stationary	815:824	arg1	phases					826:831	pseudo stationary phases	808:831	pseudo stationary phases	808:831	Native amino acids and lactic acid were separated and detected as complexes with Cu2+ ions, while proposed application of β-cyclodextrin (β-CD) and its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD) as pseudo stationary phases provided better separation selectivity.
32829136	4	59	theme	β-CDs	914:918	arg1	concentrations					920:933	β-CDs concentrations	914:933	β-CDs concentrations	914:933	The effect of CDs, Cu2+, sodium acetate, β-CDs concentrations and pH of background electrolyte (BGE) on the electrophoretic mobilities of AAs was thoroughly investigated.
32829136	7	60	theme	D76	1379:1381	arg1	strains					1383:1389	D75 and D76 strains	1371:1389	D75 and D76 strains grown together and separately	1371:1419	The differences in production of amino acids by D75 and D76 strains grown together and separately were found and concluded to be a consequence and/or one of the causes of synergism and syntropy of the strains.
32829136	3	61	theme	Cu2+	661:664	arg1	ions					666:669	Cu2+ ions	661:669	Cu2+ ions	661:669	Native amino acids and lactic acid were separated and detected as complexes with Cu2+ ions, while proposed application of β-cyclodextrin (β-CD) and its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD) as pseudo stationary phases provided better separation selectivity.
32829136	6	62	theme	high	1210:1213	arg1	analysis-to-analysis					1215:1234	high analysis-to-analysis	1210:1234	high analysis-to-analysis	1210:1234	The developed method possessed high analysis-to-analysis and day-to-day repeatability of migration times (RSD ≤ 1.0% and ≤ 2.5%, respectively).
32829136	3	63	theme	separation	849:858	arg1	selectivity					860:870	better separation selectivity	842:870	better separation selectivity	842:870	Native amino acids and lactic acid were separated and detected as complexes with Cu2+ ions, while proposed application of β-cyclodextrin (β-CD) and its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD) as pseudo stationary phases provided better separation selectivity.
32829136	3	64	with	complexes	646:654	arg1	ions					666:669	Cu2+ ions	661:669	Cu2+ ions	661:669	Native amino acids and lactic acid were separated and detected as complexes with Cu2+ ions, while proposed application of β-cyclodextrin (β-CD) and its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD) as pseudo stationary phases provided better separation selectivity.
32829136	4	65	theme	electrophoretic	981:995	arg1	mobilities					997:1006	the electrophoretic mobilities	977:1006	the electrophoretic mobilities of AAs	977:1013	The effect of CDs, Cu2+, sodium acetate, β-CDs concentrations and pH of background electrolyte (BGE) on the electrophoretic mobilities of AAs was thoroughly investigated.
32829136	7	66	theme	acids	1362:1366	arg1	production					1342:1351	production	1342:1351	production of amino acids by D75 and D76 strains grown together and separately	1342:1419	The differences in production of amino acids by D75 and D76 strains grown together and separately were found and concluded to be a consequence and/or one of the causes of synergism and syntropy of the strains.
32829136	5	67	theme	50 mM	1130:1134	arg1	follows					1090:1096	follows	1090:1096	follows	1090:1096	The composition of the BGE was found to be as follows: 20 mM acetate buffer solution, 50 mM CuSO4, 10 mM 2-hydroxypropyl-β-CD, pH 4.3.
32829136	5	67	theme	50 mM	1130:1134	arg1	CuSO4					1136:1140	50 mM CuSO4	1130:1140	50 mM CuSO4	1130:1140	The composition of the BGE was found to be as follows: 20 mM acetate buffer solution, 50 mM CuSO4, 10 mM 2-hydroxypropyl-β-CD, pH 4.3.
32829136	1	68	theme	electrophoresis	177:191	arg1	method					193:198	A capillary electrophoresis method	165:198	A capillary electrophoresis method for selective simultaneous determination and quantitation of native amino acids and lactic acid during cultivation of Lactobacillus helveticus D75 and D76 strains on the MRS-1 and milk nutrient media	165:398	A capillary electrophoresis method for selective simultaneous determination and quantitation of native amino acids and lactic acid during cultivation of Lactobacillus helveticus D75 and D76 strains on the MRS-1 and milk nutrient media was presented.
32829136	1	69	theme	nutrient	385:392	arg1	media					394:398	the MRS-1 and milk nutrient media	366:398	media	394:398	A capillary electrophoresis method for selective simultaneous determination and quantitation of native amino acids and lactic acid during cultivation of Lactobacillus helveticus D75 and D76 strains on the MRS-1 and milk nutrient media was presented.
32829136	1	70	from	method	193:198	arg1	MRS-1					370:374	the MRS-1 and milk nutrient media	366:398	MRS-1	370:374	A capillary electrophoresis method for selective simultaneous determination and quantitation of native amino acids and lactic acid during cultivation of Lactobacillus helveticus D75 and D76 strains on the MRS-1 and milk nutrient media was presented.
32829136	1	70	from	method	193:198	arg1	media					394:398	the MRS-1 and milk nutrient media	366:398	media	394:398	A capillary electrophoresis method for selective simultaneous determination and quantitation of native amino acids and lactic acid during cultivation of Lactobacillus helveticus D75 and D76 strains on the MRS-1 and milk nutrient media was presented.
32829136	2	71	theme	culture	565:571	arg1	media					573:577	culture media	565:577	culture media	565:577	The method provided sensitive UV-detection of native analytes with minimum sample preparation and appeared to be extremely useful for the analysis of culture media.
32829136	0	72	theme	Cu2+and	127:133	arg1	additives					154:162	additives	154:162	additives	154:162	Determination of native amino acids and lactic acid inLactobacillus helveticusculture media by capillary electrophoresis using Cu2+and β-cyclodextrins as additives.
32829136	0	72	theme	Cu2+and	127:133	arg1	β-cyclodextrins					135:149	Cu2+and β-cyclodextrins	127:149	Cu2+and β-cyclodextrins	127:149	Determination of native amino acids and lactic acid inLactobacillus helveticusculture media by capillary electrophoresis using Cu2+and β-cyclodextrins as additives.
32829136	5	73	theme	acetate	1105:1111	arg1	solution					1120:1127	20 mM acetate buffer solution	1099:1127	20 mM acetate buffer solution	1099:1127	The composition of the BGE was found to be as follows: 20 mM acetate buffer solution, 50 mM CuSO4, 10 mM 2-hydroxypropyl-β-CD, pH 4.3.
32829136	2	74	theme	sample	490:495	arg1	preparation					497:507	minimum sample preparation	482:507	minimum sample preparation	482:507	The method provided sensitive UV-detection of native analytes with minimum sample preparation and appeared to be extremely useful for the analysis of culture media.
32829136	8	75	theme	developed	1537:1545	arg1	method					1547:1552	The developed method	1533:1552	The developed method	1533:1552	The developed method proved to be applicable for the analysis of culture media.
32829136	8	75	theme	developed	1537:1545	arg1	applicable					1567:1576	applicable	1567:1576	applicable	1567:1576	The developed method proved to be applicable for the analysis of culture media.
32829136	0	76	theme	amino	24:28	arg1	acids					30:34	native amino acids	17:34	native amino acids	17:34	Determination of native amino acids and lactic acid inLactobacillus helveticusculture media by capillary electrophoresis using Cu2+and β-cyclodextrins as additives.
32829136	1	77	theme	helveticus	332:341	arg1	cultivation					303:313	cultivation	303:313	cultivation of Lactobacillus helveticus D75	303:345	A capillary electrophoresis method for selective simultaneous determination and quantitation of native amino acids and lactic acid during cultivation of Lactobacillus helveticus D75 and D76 strains on the MRS-1 and milk nutrient media was presented.
32829136	3	78	theme	proposed	678:685	arg1	application					687:697	proposed application	678:697	proposed application of β-cyclodextrin (β-CD) and its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD) as pseudo stationary phases	678:831	Native amino acids and lactic acid were separated and detected as complexes with Cu2+ ions, while proposed application of β-cyclodextrin (β-CD) and its charged and uncharged derivates (sulfated β-CD and 2-hydroxypropyl-β-CD) as pseudo stationary phases provided better separation selectivity.
32829136	2	79	theme	native	461:466	arg1	analytes					468:475	native analytes	461:475	native analytes	461:475	The method provided sensitive UV-detection of native analytes with minimum sample preparation and appeared to be extremely useful for the analysis of culture media.
32829136	0	80	theme	acid	47:50	arg1	Determination					0:12	Determination	0:12	Determination of native amino acids and lactic acid inLactobacillus helveticusculture media by capillary electrophoresis	0:119	Determination of native amino acids and lactic acid inLactobacillus helveticusculture media by capillary electrophoresis using Cu2+and β-cyclodextrins as additives.
32829136	7	81	theme	syntropy	1508:1515	arg1	causes					1484:1489	the causes	1480:1489	the causes of synergism and syntropy of the strains	1480:1530	The differences in production of amino acids by D75 and D76 strains grown together and separately were found and concluded to be a consequence and/or one of the causes of synergism and syntropy of the strains.
32829136	5	82	dep	follows	1090:1096	arg1	solution					1120:1127	20 mM acetate buffer solution	1099:1127	20 mM acetate buffer solution	1099:1127	The composition of the BGE was found to be as follows: 20 mM acetate buffer solution, 50 mM CuSO4, 10 mM 2-hydroxypropyl-β-CD, pH 4.3.
32829136	1	83	theme	acids	274:278	arg1	strains					355:361	D76 strains	351:361	D76 strains	351:361	A capillary electrophoresis method for selective simultaneous determination and quantitation of native amino acids and lactic acid during cultivation of Lactobacillus helveticus D75 and D76 strains on the MRS-1 and milk nutrient media was presented.
32829136	1	83	theme	acids	274:278	arg1	determination					227:239	selective simultaneous determination	204:239	selective simultaneous determination	204:239	A capillary electrophoresis method for selective simultaneous determination and quantitation of native amino acids and lactic acid during cultivation of Lactobacillus helveticus D75 and D76 strains on the MRS-1 and milk nutrient media was presented.
32829136	1	83	theme	acids	274:278	arg1	quantitation					245:256	quantitation	245:256	quantitation	245:256	A capillary electrophoresis method for selective simultaneous determination and quantitation of native amino acids and lactic acid during cultivation of Lactobacillus helveticus D75 and D76 strains on the MRS-1 and milk nutrient media was presented.
32829136	6	84	dep	times	1278:1282	arg1	%					1305:1305	RSD ≤ 1.0% and ≤ 2.5%	1285:1305	RSD ≤ 1.0% and ≤ 2.5%	1285:1305	The developed method possessed high analysis-to-analysis and day-to-day repeatability of migration times (RSD ≤ 1.0% and ≤ 2.5%, respectively).
32829136	7	85	theme	synergism	1494:1502	arg1	causes					1484:1489	the causes	1480:1489	the causes of synergism and syntropy of the strains	1480:1530	The differences in production of amino acids by D75 and D76 strains grown together and separately were found and concluded to be a consequence and/or one of the causes of synergism and syntropy of the strains.
32829136	8	86	theme	culture	1598:1604	arg1	media					1606:1610	culture media	1598:1610	culture media	1598:1610	The developed method proved to be applicable for the analysis of culture media.
32829136	1	87	theme	lactic	284:289	arg1	acid					291:294	lactic acid	284:294	lactic acid	284:294	A capillary electrophoresis method for selective simultaneous determination and quantitation of native amino acids and lactic acid during cultivation of Lactobacillus helveticus D75 and D76 strains on the MRS-1 and milk nutrient media was presented.
34974401	7	0	theme	junction	820:827	arg1	genes					829:833	intestinal tight junction genes	803:833	intestinal tight junction genes	803:833	The expression levels of intestinal tight junction genes and inflammatory genes were determined by quantitative PCR.
34974401	5	1	theme	last	664:667	arg1	4 weeks					669:675	the last 4 weeks	660:675	the last 4 weeks	660:675	RVD1 was administered i.p. during the last 4 weeks.
34974401	3	2	theme	intestinal	493:502	arg1	function					512:519	intestinal barrier function	493:519	intestinal barrier function	493:519	The aims of this study were to explore the mechanisms of RVD1 on hepatic steatosis based on the gut-liver axis and intestinal barrier function.
34974401	9	3	theme	weight	1066:1071	arg1	loss					1073:1076	weight loss	1066:1076	weight loss	1066:1076	KEY RESULTS RVD1 prevented weight loss, histopathological changes, and elevated levels of inflammatory cytokines.
34974401	9	4	theme	inflammatory	1129:1140	arg1	cytokines					1142:1150	inflammatory cytokines	1129:1150	inflammatory cytokines	1129:1150	KEY RESULTS RVD1 prevented weight loss, histopathological changes, and elevated levels of inflammatory cytokines.
34974401	4	5	dep	APPROACH	535:542	arg1	established					547:557	established	547:557	established a DSS-induced chronic colitis model to evaluate hepatic steatosis	547:623	EXPERIMENTAL APPROACH We established a DSS-induced chronic colitis model to evaluate hepatic steatosis.
34974401	3	6	theme	barrier	504:510	arg1	function					512:519	intestinal barrier function	493:519	intestinal barrier function	493:519	The aims of this study were to explore the mechanisms of RVD1 on hepatic steatosis based on the gut-liver axis and intestinal barrier function.
34974401	12	7	theme	normal	1557:1562	arg1	mice					1578:1581	normal chow diet-fed mice	1557:1581	normal chow diet-fed mice	1557:1581	The RVD1-treated mice also showed a different gut microbiota composition compared with found in the mice belonging to the DSS group but similar to that in normal chow diet-fed mice.
34974401	0	8	theme	intestinal	93:102	arg1	integrity					112:120	the intestinal barrier integrity	89:120	the intestinal barrier integrity	89:120	Resolvin D1 ameliorates hepatic steatosis by remodeling the gut microbiota and restoring the intestinal barrier integrity in DSS-induced chronic colitis.
34974401	13	9	theme	gut	1686:1688	arg1	inflammation					1690:1701	gut inflammation	1686:1701	gut inflammation	1686:1701	CONCLUSIONS AND IMPLICATIONS RVD1 treatment ameliorates DSS-induced hepatic steatosis by ameliorating gut inflammation, improving intestinal barrier function and modulating intestinal dysbiosis.
34974401	12	10	theme	gut	1448:1450	arg1	composition					1463:1473	a different gut microbiota composition	1436:1473	a different gut microbiota composition	1436:1473	The RVD1-treated mice also showed a different gut microbiota composition compared with found in the mice belonging to the DSS group but similar to that in normal chow diet-fed mice.
34974401	1	11	dep	BACKGROUND	154:163	arg1	maintenance					181:191	The maintenance	177:191	The maintenance of intestinalmucosalbarrier function	177:228	BACKGROUND AND PURPOSE The maintenance of intestinalmucosalbarrier function plays an important role in hepatic steatosis.
34974401	4	12	theme	hepatic	607:613	arg1	steatosis					615:623	hepatic steatosis	607:623	hepatic steatosis	607:623	EXPERIMENTAL APPROACH We established a DSS-induced chronic colitis model to evaluate hepatic steatosis.
34974401	6	13	theme	histopathological	750:766	arg1	analysis					768:775	histopathological analysis	750:775	histopathological analysis	750:775	The colon and liver samples were stained with hematoxylin and eosin for histopathological analysis.
34974401	8	14	theme	triglycerides	937:949	arg1	levels					905:910	The serum levels	895:910	The serum levels of glucose, cholesterol, triglycerides and LPS	895:957	The serum levels of glucose, cholesterol, triglycerides and LPS were measured, and the gut microbiota was analyzed by 16S rRNA gene sequencing.
34974401	12	15	theme	different	1438:1446	arg1	composition					1463:1473	a different gut microbiota composition	1436:1473	a different gut microbiota composition	1436:1473	The RVD1-treated mice also showed a different gut microbiota composition compared with found in the mice belonging to the DSS group but similar to that in normal chow diet-fed mice.
34974401	2	16	theme	resolvin	311:318	arg1	RVD1					324:327	RVD1	324:327	RVD1	324:327	Increasing evidence has shown that resolvin D1 (RVD1) exerts a potential effect on hepatic steatosis.
34974401	2	16	theme	resolvin	311:318	arg1	D1					320:321	resolvin D1	311:321	resolvin D1 (RVD1)	311:328	Increasing evidence has shown that resolvin D1 (RVD1) exerts a potential effect on hepatic steatosis.
34974401	9	17	theme	histopathological	1079:1095	arg1	changes					1097:1103	histopathological changes	1079:1103	histopathological changes	1079:1103	KEY RESULTS RVD1 prevented weight loss, histopathological changes, and elevated levels of inflammatory cytokines.
34974401	12	18	theme	diet-fed	1569:1576	arg1	mice					1578:1581	normal chow diet-fed mice	1557:1581	normal chow diet-fed mice	1557:1581	The RVD1-treated mice also showed a different gut microbiota composition compared with found in the mice belonging to the DSS group but similar to that in normal chow diet-fed mice.
34974401	11	19	theme	molecules	1354:1362	arg1	levels					1329:1334	levels	1329:1334	levels of tight junction molecules	1329:1362	In addition, RVD1 improved intestinal barrier function by increasing levels of tight junction molecules and decreasing the plasma LPS levels.
34974401	10	20	theme	DSS-induced	1194:1204	arg1	steatosis					1214:1222	DSS-induced hepatic steatosis	1194:1222	DSS-induced hepatic steatosis	1194:1222	Moreover, RVD1 administration attenuated DSS-induced hepatic steatosis and inflammatory responses in mice.
34974401	8	21	theme	serum	899:903	arg1	levels					905:910	The serum levels	895:910	The serum levels of glucose, cholesterol, triglycerides and LPS	895:957	The serum levels of glucose, cholesterol, triglycerides and LPS were measured, and the gut microbiota was analyzed by 16S rRNA gene sequencing.
34974401	3	22	theme	RVD1	435:438	arg1	mechanisms					421:430	the mechanisms	417:430	the mechanisms of RVD1 on hepatic steatosis based on the gut-liver axis and intestinal barrier function	417:519	The aims of this study were to explore the mechanisms of RVD1 on hepatic steatosis based on the gut-liver axis and intestinal barrier function.
34974401	0	23	theme	barrier	104:110	arg1	integrity					112:120	the intestinal barrier integrity	89:120	the intestinal barrier integrity	89:120	Resolvin D1 ameliorates hepatic steatosis by remodeling the gut microbiota and restoring the intestinal barrier integrity in DSS-induced chronic colitis.
34974401	12	24	theme	chow	1564:1567	arg1	mice					1578:1581	normal chow diet-fed mice	1557:1581	normal chow diet-fed mice	1557:1581	The RVD1-treated mice also showed a different gut microbiota composition compared with found in the mice belonging to the DSS group but similar to that in normal chow diet-fed mice.
34974401	8	25	theme	rRNA	1017:1020	arg1	sequencing					1027:1036	rRNA gene sequencing	1017:1036	rRNA gene sequencing	1017:1036	The serum levels of glucose, cholesterol, triglycerides and LPS were measured, and the gut microbiota was analyzed by 16S rRNA gene sequencing.
34974401	0	26	theme	Resolvin	0:7	arg1	D1					9:10	Resolvin D1	0:10	Resolvin D1	0:10	Resolvin D1 ameliorates hepatic steatosis by remodeling the gut microbiota and restoring the intestinal barrier integrity in DSS-induced chronic colitis.
34974401	7	27	theme	quantitative	877:888	arg1	PCR					890:892	quantitative PCR	877:892	quantitative PCR	877:892	The expression levels of intestinal tight junction genes and inflammatory genes were determined by quantitative PCR.
34974401	13	28	theme	DSS-induced	1640:1650	arg1	steatosis					1660:1668	DSS-induced hepatic steatosis	1640:1668	DSS-induced hepatic steatosis	1640:1668	CONCLUSIONS AND IMPLICATIONS RVD1 treatment ameliorates DSS-induced hepatic steatosis by ameliorating gut inflammation, improving intestinal barrier function and modulating intestinal dysbiosis.
34974401	11	29	theme	plasma	1383:1388	arg1	levels					1394:1399	the plasma LPS levels	1379:1399	the plasma LPS levels	1379:1399	In addition, RVD1 improved intestinal barrier function by increasing levels of tight junction molecules and decreasing the plasma LPS levels.
34974401	7	30	theme	genes	829:833	arg1	levels					793:798	The expression levels	778:798	The expression levels of intestinal tight junction genes and inflammatory genes	778:856	The expression levels of intestinal tight junction genes and inflammatory genes were determined by quantitative PCR.
34974401	11	31	theme	tight	1339:1343	arg1	molecules					1354:1362	tight junction molecules	1339:1362	tight junction molecules	1339:1362	In addition, RVD1 improved intestinal barrier function by increasing levels of tight junction molecules and decreasing the plasma LPS levels.
34974401	13	32	theme	RVD1	1613:1616	arg1	treatment					1618:1626	CONCLUSIONS AND IMPLICATIONS RVD1 treatment	1584:1626	CONCLUSIONS AND IMPLICATIONS RVD1 treatment	1584:1626	CONCLUSIONS AND IMPLICATIONS RVD1 treatment ameliorates DSS-induced hepatic steatosis by ameliorating gut inflammation, improving intestinal barrier function and modulating intestinal dysbiosis.
34974401	10	33	from	responses	1241:1249	arg1	mice					1254:1257	mice	1254:1257	mice	1254:1257	Moreover, RVD1 administration attenuated DSS-induced hepatic steatosis and inflammatory responses in mice.
34974401	12	34	theme	microbiota	1452:1461	arg1	composition					1463:1473	a different gut microbiota composition	1436:1473	a different gut microbiota composition	1436:1473	The RVD1-treated mice also showed a different gut microbiota composition compared with found in the mice belonging to the DSS group but similar to that in normal chow diet-fed mice.
34974401	3	35	theme	study	395:399	arg1	aims					382:385	The aims	378:385	The aims of this study	378:399	The aims of this study were to explore the mechanisms of RVD1 on hepatic steatosis based on the gut-liver axis and intestinal barrier function.
34974401	4	36	theme	DSS-induced	561:571	arg1	model					589:593	a DSS-induced chronic colitis model	559:593	a DSS-induced chronic colitis model to evaluate hepatic steatosis	559:623	EXPERIMENTAL APPROACH We established a DSS-induced chronic colitis model to evaluate hepatic steatosis.
34974401	11	37	theme	junction	1345:1352	arg1	molecules					1354:1362	tight junction molecules	1339:1362	tight junction molecules	1339:1362	In addition, RVD1 improved intestinal barrier function by increasing levels of tight junction molecules and decreasing the plasma LPS levels.
34974401	11	38	theme	LPS	1390:1392	arg1	levels					1394:1399	the plasma LPS levels	1379:1399	the plasma LPS levels	1379:1399	In addition, RVD1 improved intestinal barrier function by increasing levels of tight junction molecules and decreasing the plasma LPS levels.
34974401	8	39	theme	cholesterol	924:934	arg1	levels					905:910	The serum levels	895:910	The serum levels of glucose, cholesterol, triglycerides and LPS	895:957	The serum levels of glucose, cholesterol, triglycerides and LPS were measured, and the gut microbiota was analyzed by 16S rRNA gene sequencing.
34974401	1	40	theme	intestinalmucosalbarrier	196:219	arg1	function					221:228	intestinalmucosalbarrier function	196:228	intestinalmucosalbarrier function	196:228	BACKGROUND AND PURPOSE The maintenance of intestinalmucosalbarrier function plays an important role in hepatic steatosis.
34974401	0	41	theme	hepatic	24:30	arg1	steatosis					32:40	hepatic steatosis	24:40	hepatic steatosis	24:40	Resolvin D1 ameliorates hepatic steatosis by remodeling the gut microbiota and restoring the intestinal barrier integrity in DSS-induced chronic colitis.
34974401	7	42	theme	inflammatory	839:850	arg1	genes					852:856	inflammatory genes	839:856	inflammatory genes	839:856	The expression levels of intestinal tight junction genes and inflammatory genes were determined by quantitative PCR.
34974401	0	43	theme	DSS-induced	125:135	arg1	colitis					145:151	DSS-induced chronic colitis	125:151	DSS-induced chronic colitis	125:151	Resolvin D1 ameliorates hepatic steatosis by remodeling the gut microbiota and restoring the intestinal barrier integrity in DSS-induced chronic colitis.
34974401	8	44	theme	gene	1022:1025	arg1	sequencing					1027:1036	rRNA gene sequencing	1017:1036	rRNA gene sequencing	1017:1036	The serum levels of glucose, cholesterol, triglycerides and LPS were measured, and the gut microbiota was analyzed by 16S rRNA gene sequencing.
34974401	6	45	theme	liver	692:696	arg1	samples					698:704	The colon and liver samples	678:704	The colon and liver samples	678:704	The colon and liver samples were stained with hematoxylin and eosin for histopathological analysis.
34974401	1	46	theme	function	221:228	arg1	maintenance					181:191	The maintenance	177:191	The maintenance of intestinalmucosalbarrier function	177:228	BACKGROUND AND PURPOSE The maintenance of intestinalmucosalbarrier function plays an important role in hepatic steatosis.
34974401	4	47	theme	colitis	581:587	arg1	model					589:593	a DSS-induced chronic colitis model	559:593	a DSS-induced chronic colitis model to evaluate hepatic steatosis	559:623	EXPERIMENTAL APPROACH We established a DSS-induced chronic colitis model to evaluate hepatic steatosis.
34974401	13	48	theme	barrier	1725:1731	arg1	function					1733:1740	intestinal barrier function	1714:1740	intestinal barrier function	1714:1740	CONCLUSIONS AND IMPLICATIONS RVD1 treatment ameliorates DSS-induced hepatic steatosis by ameliorating gut inflammation, improving intestinal barrier function and modulating intestinal dysbiosis.
34974401	2	49	theme	hepatic	359:365	arg1	steatosis					367:375	hepatic steatosis	359:375	hepatic steatosis	359:375	Increasing evidence has shown that resolvin D1 (RVD1) exerts a potential effect on hepatic steatosis.
34974401	7	50	theme	genes	852:856	arg1	levels					793:798	The expression levels	778:798	The expression levels of intestinal tight junction genes and inflammatory genes	778:856	The expression levels of intestinal tight junction genes and inflammatory genes were determined by quantitative PCR.
34974401	9	51	theme	elevated	1110:1117	arg1	levels					1119:1124	elevated levels	1110:1124	elevated levels of inflammatory cytokines	1110:1150	KEY RESULTS RVD1 prevented weight loss, histopathological changes, and elevated levels of inflammatory cytokines.
34974401	13	52	theme	intestinal	1714:1723	arg1	function					1733:1740	intestinal barrier function	1714:1740	intestinal barrier function	1714:1740	CONCLUSIONS AND IMPLICATIONS RVD1 treatment ameliorates DSS-induced hepatic steatosis by ameliorating gut inflammation, improving intestinal barrier function and modulating intestinal dysbiosis.
34974401	4	53	theme	chronic	573:579	arg1	model					589:593	a DSS-induced chronic colitis model	559:593	a DSS-induced chronic colitis model to evaluate hepatic steatosis	559:623	EXPERIMENTAL APPROACH We established a DSS-induced chronic colitis model to evaluate hepatic steatosis.
34974401	6	54	theme	colon	682:686	arg1	samples					698:704	The colon and liver samples	678:704	The colon and liver samples	678:704	The colon and liver samples were stained with hematoxylin and eosin for histopathological analysis.
34974401	3	55	theme	hepatic	443:449	arg1	steatosis					451:459	hepatic steatosis	443:459	hepatic steatosis based on the gut-liver axis and intestinal barrier function	443:519	The aims of this study were to explore the mechanisms of RVD1 on hepatic steatosis based on the gut-liver axis and intestinal barrier function.
34974401	7	56	theme	intestinal	803:812	arg1	genes					829:833	intestinal tight junction genes	803:833	intestinal tight junction genes	803:833	The expression levels of intestinal tight junction genes and inflammatory genes were determined by quantitative PCR.
34974401	0	57	theme	chronic	137:143	arg1	colitis					145:151	DSS-induced chronic colitis	125:151	DSS-induced chronic colitis	125:151	Resolvin D1 ameliorates hepatic steatosis by remodeling the gut microbiota and restoring the intestinal barrier integrity in DSS-induced chronic colitis.
34974401	13	58	theme	CONCLUSIONS	1584:1594	arg1	treatment					1618:1626	CONCLUSIONS AND IMPLICATIONS RVD1 treatment	1584:1626	CONCLUSIONS AND IMPLICATIONS RVD1 treatment	1584:1626	CONCLUSIONS AND IMPLICATIONS RVD1 treatment ameliorates DSS-induced hepatic steatosis by ameliorating gut inflammation, improving intestinal barrier function and modulating intestinal dysbiosis.
34974401	8	59	theme	LPS	955:957	arg1	levels					905:910	The serum levels	895:910	The serum levels of glucose, cholesterol, triglycerides and LPS	895:957	The serum levels of glucose, cholesterol, triglycerides and LPS were measured, and the gut microbiota was analyzed by 16S rRNA gene sequencing.
34974401	1	60	theme	important	239:247	arg1	role					249:252	an important role	236:252	an important role	236:252	BACKGROUND AND PURPOSE The maintenance of intestinalmucosalbarrier function plays an important role in hepatic steatosis.
34974401	4	61	theme	EXPERIMENTAL	522:533	arg1	APPROACH					535:542	EXPERIMENTAL APPROACH	522:542	EXPERIMENTAL APPROACH We established a DSS-induced chronic colitis model to evaluate hepatic steatosis.	522:624	EXPERIMENTAL APPROACH We established a DSS-induced chronic colitis model to evaluate hepatic steatosis.
34974401	11	62	theme	barrier	1298:1304	arg1	function					1306:1313	intestinal barrier function	1287:1313	intestinal barrier function	1287:1313	In addition, RVD1 improved intestinal barrier function by increasing levels of tight junction molecules and decreasing the plasma LPS levels.
34974401	10	63	theme	hepatic	1206:1212	arg1	steatosis					1214:1222	DSS-induced hepatic steatosis	1194:1222	DSS-induced hepatic steatosis	1194:1222	Moreover, RVD1 administration attenuated DSS-induced hepatic steatosis and inflammatory responses in mice.
34974401	12	64	theme	RVD1-treated	1406:1417	arg1	mice					1419:1422	The RVD1-treated mice	1402:1422	The RVD1-treated mice	1402:1422	The RVD1-treated mice also showed a different gut microbiota composition compared with found in the mice belonging to the DSS group but similar to that in normal chow diet-fed mice.
34974401	13	65	theme	intestinal	1757:1766	arg1	dysbiosis					1768:1776	intestinal dysbiosis	1757:1776	intestinal dysbiosis	1757:1776	CONCLUSIONS AND IMPLICATIONS RVD1 treatment ameliorates DSS-induced hepatic steatosis by ameliorating gut inflammation, improving intestinal barrier function and modulating intestinal dysbiosis.
34974401	10	66	from	steatosis	1214:1222	arg1	mice					1254:1257	mice	1254:1257	mice	1254:1257	Moreover, RVD1 administration attenuated DSS-induced hepatic steatosis and inflammatory responses in mice.
34974401	12	67	theme	DSS	1524:1526	arg1	group					1528:1532	the DSS group	1520:1532	the DSS group	1520:1532	The RVD1-treated mice also showed a different gut microbiota composition compared with found in the mice belonging to the DSS group but similar to that in normal chow diet-fed mice.
34974401	13	68	theme	IMPLICATIONS	1600:1611	arg1	treatment					1618:1626	CONCLUSIONS AND IMPLICATIONS RVD1 treatment	1584:1626	CONCLUSIONS AND IMPLICATIONS RVD1 treatment	1584:1626	CONCLUSIONS AND IMPLICATIONS RVD1 treatment ameliorates DSS-induced hepatic steatosis by ameliorating gut inflammation, improving intestinal barrier function and modulating intestinal dysbiosis.
34974401	10	69	theme	RVD1	1163:1166	arg1	administration					1168:1181	RVD1 administration	1163:1181	RVD1 administration	1163:1181	Moreover, RVD1 administration attenuated DSS-induced hepatic steatosis and inflammatory responses in mice.
34974401	2	70	theme	potential	339:347	arg1	effect					349:354	a potential effect	337:354	a potential effect	337:354	Increasing evidence has shown that resolvin D1 (RVD1) exerts a potential effect on hepatic steatosis.
34974401	9	71	theme	KEY	1039:1041	arg1	RESULTS					1043:1049	KEY RESULTS	1039:1049	KEY RESULTS	1039:1049	KEY RESULTS RVD1 prevented weight loss, histopathological changes, and elevated levels of inflammatory cytokines.
34974401	10	72	theme	inflammatory	1228:1239	arg1	responses					1241:1249	inflammatory responses	1228:1249	inflammatory responses in mice	1228:1257	Moreover, RVD1 administration attenuated DSS-induced hepatic steatosis and inflammatory responses in mice.
34974401	11	73	theme	intestinal	1287:1296	arg1	function					1306:1313	intestinal barrier function	1287:1313	intestinal barrier function	1287:1313	In addition, RVD1 improved intestinal barrier function by increasing levels of tight junction molecules and decreasing the plasma LPS levels.
34974401	9	74	theme	cytokines	1142:1150	arg1	levels					1119:1124	elevated levels	1110:1124	elevated levels of inflammatory cytokines	1110:1150	KEY RESULTS RVD1 prevented weight loss, histopathological changes, and elevated levels of inflammatory cytokines.
34974401	9	74	theme	cytokines	1142:1150	arg1	loss					1073:1076	weight loss	1066:1076	weight loss	1066:1076	KEY RESULTS RVD1 prevented weight loss, histopathological changes, and elevated levels of inflammatory cytokines.
34974401	9	74	theme	cytokines	1142:1150	arg1	changes					1097:1103	histopathological changes	1079:1103	histopathological changes	1079:1103	KEY RESULTS RVD1 prevented weight loss, histopathological changes, and elevated levels of inflammatory cytokines.
34974401	3	75	from	mechanisms	421:430	arg1	steatosis					451:459	hepatic steatosis	443:459	hepatic steatosis based on the gut-liver axis and intestinal barrier function	443:519	The aims of this study were to explore the mechanisms of RVD1 on hepatic steatosis based on the gut-liver axis and intestinal barrier function.
34974401	3	76	theme	gut-liver	474:482	arg1	axis					484:487	the gut-liver axis	470:487	the gut-liver axis	470:487	The aims of this study were to explore the mechanisms of RVD1 on hepatic steatosis based on the gut-liver axis and intestinal barrier function.
34974401	7	77	theme	expression	782:791	arg1	levels					793:798	The expression levels	778:798	The expression levels of intestinal tight junction genes and inflammatory genes	778:856	The expression levels of intestinal tight junction genes and inflammatory genes were determined by quantitative PCR.
34974401	2	78	theme	Increasing	276:285	arg1	evidence					287:294	Increasing evidence	276:294	Increasing evidence	276:294	Increasing evidence has shown that resolvin D1 (RVD1) exerts a potential effect on hepatic steatosis.
34974401	1	79	theme	hepatic	257:263	arg1	steatosis					265:273	hepatic steatosis	257:273	hepatic steatosis	257:273	BACKGROUND AND PURPOSE The maintenance of intestinalmucosalbarrier function plays an important role in hepatic steatosis.
34974401	0	80	theme	gut	60:62	arg1	microbiota					64:73	the gut microbiota	56:73	the gut microbiota	56:73	Resolvin D1 ameliorates hepatic steatosis by remodeling the gut microbiota and restoring the intestinal barrier integrity in DSS-induced chronic colitis.
34974401	13	81	theme	hepatic	1652:1658	arg1	steatosis					1660:1668	DSS-induced hepatic steatosis	1640:1668	DSS-induced hepatic steatosis	1640:1668	CONCLUSIONS AND IMPLICATIONS RVD1 treatment ameliorates DSS-induced hepatic steatosis by ameliorating gut inflammation, improving intestinal barrier function and modulating intestinal dysbiosis.
34974401	8	82	theme	glucose	915:921	arg1	levels					905:910	The serum levels	895:910	The serum levels of glucose, cholesterol, triglycerides and LPS	895:957	The serum levels of glucose, cholesterol, triglycerides and LPS were measured, and the gut microbiota was analyzed by 16S rRNA gene sequencing.
34974401	8	83	theme	gut	982:984	arg1	microbiota					986:995	the gut microbiota	978:995	the gut microbiota	978:995	The serum levels of glucose, cholesterol, triglycerides and LPS were measured, and the gut microbiota was analyzed by 16S rRNA gene sequencing.
34974401	7	84	theme	tight	814:818	arg1	genes					829:833	intestinal tight junction genes	803:833	intestinal tight junction genes	803:833	The expression levels of intestinal tight junction genes and inflammatory genes were determined by quantitative PCR.
33232699	4	0	dep	PDA	1267:1269	arg1	chelation					1278:1286	chelation	1278:1286	chelation	1278:1286	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	2	1	theme	fiber	554:558	arg1	surface					560:566	the composite fiber surface	540:566	the composite fiber surface to construct the adhesive polydopamine (PDA) film	540:616	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	4	2	theme	strong	1248:1253	arg1	adhesion					1255:1262	the strong adhesion	1244:1262	the strong adhesion of PDA and CS chelation	1244:1286	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	2	3	theme	acid	354:357	arg1	fiber					388:392	a polylactic acid (PLLA)/silver (Ag) composite fiber	341:392	a polylactic acid (PLLA)/silver (Ag) composite fiber	341:392	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	3	4	theme	antibacterial	1099:1111	arg1	effect					1113:1118	an excellent antibacterial effect	1086:1118	an excellent antibacterial effect against E. coli and S. aureus	1086:1148	The study results showed that the composite fiber has an excellent antibacterial effect against E. coli and S. aureus, and good osteoinductive and angiogenic properties.
33232699	2	5	theme	Ag-NPs	743:748	arg1	coating					750:756	a stable Ag-NPs coating	734:756	a stable Ag-NPs coating (CS/Ag)	734:764	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	2	5	theme	Ag-NPs	743:748	arg1	CS/Ag					759:763	CS/Ag	759:763	CS/Ag	759:763	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	4	6	theme	dual	1224:1227	arg1	regulations					1229:1239	the dual regulations	1220:1239	the dual regulations of the strong adhesion of PDA and CS chelation	1220:1286	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	3	7	theme	good	1155:1158	arg1	properties					1190:1199	good osteoinductive and angiogenic properties	1155:1199	good osteoinductive and angiogenic properties	1155:1199	The study results showed that the composite fiber has an excellent antibacterial effect against E. coli and S. aureus, and good osteoinductive and angiogenic properties.
33232699	2	8	theme	electrospinning	295:309	arg1	technique					311:319	the electrospinning technique	291:319	the electrospinning technique	291:319	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	1	9	theme	tissue	222:227	arg1	problem					267:273	a clinical problem	256:273	a clinical problem	256:273	Bone infections caused by bacteria during bone graft implantations can impair the ability of bone tissue repair, which is currently a clinical problem.
33232699	1	9	theme	tissue	222:227	arg1	repair					229:234	bone tissue repair	217:234	bone tissue repair	217:234	Bone infections caused by bacteria during bone graft implantations can impair the ability of bone tissue repair, which is currently a clinical problem.
33232699	2	10	theme	composite	911:919	arg1	fiber					921:925	a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber	860:925	a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability	860:1029	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	2	11	theme	/silver	365:371	arg1	fiber					388:392	a polylactic acid (PLLA)/silver (Ag) composite fiber	341:392	a polylactic acid (PLLA)/silver (Ag) composite fiber	341:392	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	2	12	theme	@	904:904	arg1	fiber					921:925	a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber	860:925	a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability	860:1029	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	0	13	theme	long-term	77:85	arg1	antibacterial					87:99	long-term antibacterial	77:99	long-term antibacterial	77:99	Inside-outside Ag nanoparticles-loaded polylactic acid electrospun fiber for long-term antibacterial and bone regeneration.
33232699	1	14	theme	graft	171:175	arg1	implantations					177:189	bone graft implantations	166:189	bone graft implantations	166:189	Bone infections caused by bacteria during bone graft implantations can impair the ability of bone tissue repair, which is currently a clinical problem.
33232699	4	15	theme	infection	1427:1435	arg1	ability					1448:1454	bone infection inhibition ability	1422:1454	bone infection inhibition ability	1422:1454	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	2	16	theme	inside-outside	863:876	arg1	fiber					921:925	a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber	860:925	a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability	860:1029	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	4	17	theme	inside-outside	1294:1307	arg1	fiber					1334:1338	the "inside-outside" Ag-NPs-loaded composite fiber	1289:1338	the "inside-outside" Ag-NPs-loaded composite fiber	1289:1338	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	4	17	theme	inside-outside	1294:1307	arg1	endowed					1344:1350	endowed	1344:1350	endowed	1344:1350	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	2	18	theme	PLLA	489:492	arg1	fibers					494:499	PLLA fibers	489:499	PLLA fibers	489:499	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	4	19	theme	Ag-NPs-loaded	1310:1322	arg1	fiber					1334:1338	the "inside-outside" Ag-NPs-loaded composite fiber	1289:1338	the "inside-outside" Ag-NPs-loaded composite fiber	1289:1338	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	4	19	theme	Ag-NPs-loaded	1310:1322	arg1	endowed					1344:1350	endowed	1344:1350	endowed	1344:1350	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	2	20	theme	@	900:900	arg1	fiber					921:925	a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber	860:925	a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability	860:1029	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	2	21	theme	adhesive	585:592	arg1	film					613:616	the adhesive polydopamine (PDA) film	581:616	the adhesive polydopamine (PDA) film	581:616	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	4	22	theme	long-term	1387:1395	arg1	effect					1411:1416	long-term antibacterial effect	1387:1416	long-term antibacterial effect	1387:1416	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	2	23	theme	Ag-NPs-loaded	879:891	arg1	fiber					921:925	a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber	860:925	a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability	860:1029	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	0	24	theme	Inside-outside	0:13	arg1	Ag					15:16	Inside-outside Ag	0:16	Inside-outside Ag	0:16	Inside-outside Ag nanoparticles-loaded polylactic acid electrospun fiber for long-term antibacterial and bone regeneration.
33232699	4	25	from	summary	1205:1211	arg1	fiber					1334:1338	the "inside-outside" Ag-NPs-loaded composite fiber	1289:1338	the "inside-outside" Ag-NPs-loaded composite fiber	1289:1338	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	4	25	from	summary	1205:1211	arg1	endowed					1344:1350	endowed	1344:1350	endowed	1344:1350	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	4	26	theme	good	1357:1360	arg1	stability					1376:1384	good physiological stability	1357:1384	good physiological stability	1357:1384	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	4	27	with	endowed	1344:1350	arg1	stability					1376:1384	good physiological stability	1357:1384	good physiological stability	1357:1384	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	4	27	with	endowed	1344:1350	arg1	ability					1448:1454	bone infection inhibition ability	1422:1454	bone infection inhibition ability	1422:1454	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	4	27	with	endowed	1344:1350	arg1	effect					1411:1416	long-term antibacterial effect	1387:1416	long-term antibacterial effect	1387:1416	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	0	28	theme	acid	50:53	arg1	fiber					67:71	polylactic acid electrospun fiber	39:71	polylactic acid electrospun fiber	39:71	Inside-outside Ag nanoparticles-loaded polylactic acid electrospun fiber for long-term antibacterial and bone regeneration.
33232699	2	29	theme	antibacterial	963:975	arg1	effect					977:982	long-lasting antibacterial effect	950:982	long-lasting antibacterial effect	950:982	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	2	30	theme	polylactic	343:352	arg1	fiber					388:392	a polylactic acid (PLLA)/silver (Ag) composite fiber	341:392	a polylactic acid (PLLA)/silver (Ag) composite fiber	341:392	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	2	31	theme	stable	736:741	arg1	coating					750:756	a stable Ag-NPs coating	734:756	a stable Ag-NPs coating (CS/Ag)	734:764	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	2	31	theme	stable	736:741	arg1	CS/Ag					759:763	CS/Ag	759:763	CS/Ag	759:763	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	2	32	theme	regeneration	1000:1011	arg1	ability					1023:1029	bone regeneration promotion ability	995:1029	bone regeneration promotion ability	995:1029	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	4	33	from	endowed	1344:1350	arg1	summary					1205:1211	summary	1205:1211	summary	1205:1211	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	4	34	theme	physiological	1362:1374	arg1	stability					1376:1384	good physiological stability	1357:1384	good physiological stability	1357:1384	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	4	35	theme	bone	1422:1425	arg1	ability					1448:1454	bone infection inhibition ability	1422:1454	bone infection inhibition ability	1422:1454	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	4	36	theme	inhibition	1437:1446	arg1	ability					1448:1454	bone infection inhibition ability	1422:1454	bone infection inhibition ability	1422:1454	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	1	37	theme	clinical	258:265	arg1	problem					267:273	a clinical problem	256:273	a clinical problem	256:273	Bone infections caused by bacteria during bone graft implantations can impair the ability of bone tissue repair, which is currently a clinical problem.
33232699	1	37	theme	clinical	258:265	arg1	repair					229:234	bone tissue repair	217:234	bone tissue repair	217:234	Bone infections caused by bacteria during bone graft implantations can impair the ability of bone tissue repair, which is currently a clinical problem.
33232699	3	38	theme	composite	1066:1074	arg1	fiber					1076:1080	the composite fiber	1062:1080	the composite fiber	1062:1080	The study results showed that the composite fiber has an excellent antibacterial effect against E. coli and S. aureus, and good osteoinductive and angiogenic properties.
33232699	2	39	theme	composite	544:552	arg1	surface					560:566	the composite fiber surface	540:566	the composite fiber surface to construct the adhesive polydopamine (PDA) film	540:616	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	2	40	theme	coating	750:756	arg1	construction					718:729	the construction	714:729	the construction	714:729	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	2	41	theme	slow	792:795	arg1	release					797:803	the steady and slow release	777:803	the steady and slow release of Ag-NPs	777:813	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	2	42	theme	pulse	677:681	arg1	deposition					699:708	pulse electrochemical deposition	677:708	pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability	677:1029	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	2	43	theme	steady	781:786	arg1	release					797:803	the steady and slow release	777:803	the steady and slow release of Ag-NPs	777:813	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	3	44	theme	excellent	1089:1097	arg1	effect					1113:1118	an excellent antibacterial effect	1086:1118	an excellent antibacterial effect against E. coli and S. aureus	1086:1148	The study results showed that the composite fiber has an excellent antibacterial effect against E. coli and S. aureus, and good osteoinductive and angiogenic properties.
33232699	3	45	theme	angiogenic	1179:1188	arg1	properties					1190:1199	good osteoinductive and angiogenic properties	1155:1199	good osteoinductive and angiogenic properties	1155:1199	The study results showed that the composite fiber has an excellent antibacterial effect against E. coli and S. aureus, and good osteoinductive and angiogenic properties.
33232699	4	46	theme	promising	1469:1477	arg1	material					1492:1499	a promising bone implant material	1467:1499	a promising bone implant material	1467:1499	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	2	47	dep	surface	560:566	arg1	construct					571:579	construct	571:579	to construct the adhesive polydopamine (PDA) film	568:616	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	3	48	theme	osteoinductive	1160:1173	arg1	properties					1190:1199	good osteoinductive and angiogenic properties	1155:1199	good osteoinductive and angiogenic properties	1155:1199	The study results showed that the composite fiber has an excellent antibacterial effect against E. coli and S. aureus, and good osteoinductive and angiogenic properties.
33232699	4	49	theme	CS	1275:1276	arg1	adhesion					1255:1262	the strong adhesion	1244:1262	the strong adhesion of PDA and CS chelation	1244:1286	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	4	50	theme	implant	1484:1490	arg1	material					1492:1499	a promising bone implant material	1467:1499	a promising bone implant material	1467:1499	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	1	51	theme	bone	217:220	arg1	problem					267:273	a clinical problem	256:273	a clinical problem	256:273	Bone infections caused by bacteria during bone graft implantations can impair the ability of bone tissue repair, which is currently a clinical problem.
33232699	1	51	theme	bone	217:220	arg1	repair					229:234	bone tissue repair	217:234	bone tissue repair	217:234	Bone infections caused by bacteria during bone graft implantations can impair the ability of bone tissue repair, which is currently a clinical problem.
33232699	2	52	theme	fiber	921:925	arg1	construction					844:855	the construction	840:855	the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability	840:1029	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	1	53	theme	repair	229:234	arg1	ability					206:212	the ability	202:212	the ability of bone tissue repair, which is currently a clinical problem	202:273	Bone infections caused by bacteria during bone graft implantations can impair the ability of bone tissue repair, which is currently a clinical problem.
33232699	2	54	theme	fibers	494:499	arg1	surface					478:484	the inner surface	468:484	the inner surface of PLLA fibers	468:499	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	2	55	theme	CS/Ag	905:909	arg1	fiber					921:925	a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber	860:925	a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability	860:1029	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	4	56	theme	adhesion	1255:1262	arg1	regulations					1229:1239	the dual regulations	1220:1239	the dual regulations of the strong adhesion of PDA and CS chelation	1220:1286	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	4	57	theme	PDA	1267:1269	arg1	adhesion					1255:1262	the strong adhesion	1244:1262	the strong adhesion of PDA and CS chelation	1244:1286	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	2	58	with	fiber	921:925	arg1	functions					937:945	dual functions	932:945	dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability	932:1029	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	2	59	theme	dual	932:935	arg1	functions					937:945	dual functions	932:945	dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability	932:1029	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	4	60	theme	composite	1324:1332	arg1	fiber					1334:1338	the "inside-outside" Ag-NPs-loaded composite fiber	1289:1338	the "inside-outside" Ag-NPs-loaded composite fiber	1289:1338	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	4	60	theme	composite	1324:1332	arg1	endowed					1344:1350	endowed	1344:1350	endowed	1344:1350	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	1	61	theme	bone	166:169	arg1	implantations					177:189	bone graft implantations	166:189	bone graft implantations	166:189	Bone infections caused by bacteria during bone graft implantations can impair the ability of bone tissue repair, which is currently a clinical problem.
33232699	2	62	theme	"	877:877	arg1	fiber					921:925	a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber	860:925	a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability	860:1029	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	3	63	contain	has	1082:1084	arg1	fiber					1076:1080	the composite fiber	1062:1080	the composite fiber	1062:1080	The study results showed that the composite fiber has an excellent antibacterial effect against E. coli and S. aureus, and good osteoinductive and angiogenic properties.
33232699	3	63	contain	has	1082:1084	arg2	effect					1113:1118	an excellent antibacterial effect	1086:1118	an excellent antibacterial effect against E. coli and S. aureus	1086:1148	The study results showed that the composite fiber has an excellent antibacterial effect against E. coli and S. aureus, and good osteoinductive and angiogenic properties.
33232699	0	64	theme	bone	105:108	arg1	regeneration					110:121	bone regeneration	105:121	bone regeneration	105:121	Inside-outside Ag nanoparticles-loaded polylactic acid electrospun fiber for long-term antibacterial and bone regeneration.
33232699	2	65	theme	PDA	608:610	arg1	film					613:616	the adhesive polydopamine (PDA) film	581:616	the adhesive polydopamine (PDA) film	581:616	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	4	66	theme	bone	1479:1482	arg1	material					1492:1499	a promising bone implant material	1467:1499	a promising bone implant material	1467:1499	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	2	67	theme	PDA	901:903	arg1	fiber					921:925	a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber	860:925	a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability	860:1029	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	2	68	theme	polydopamine	594:605	arg1	film					613:616	the adhesive polydopamine (PDA) film	581:616	the adhesive polydopamine (PDA) film	581:616	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	4	69	theme	"	1308:1308	arg1	fiber					1334:1338	the "inside-outside" Ag-NPs-loaded composite fiber	1289:1338	the "inside-outside" Ag-NPs-loaded composite fiber	1289:1338	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	4	69	theme	"	1308:1308	arg1	endowed					1344:1350	endowed	1344:1350	endowed	1344:1350	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	2	70	theme	inner	472:476	arg1	surface					478:484	the inner surface	468:484	the inner surface of PLLA fibers	468:499	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	2	71	theme	PLLA/Ag	893:899	arg1	fiber					921:925	a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber	860:925	a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability	860:1029	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	2	72	theme	promotion	1013:1021	arg1	ability					1023:1029	bone regeneration promotion ability	995:1029	bone regeneration promotion ability	995:1029	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	2	73	theme	Ag	374:375	arg1	fiber					388:392	a polylactic acid (PLLA)/silver (Ag) composite fiber	341:392	a polylactic acid (PLLA)/silver (Ag) composite fiber	341:392	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	2	74	theme	silver	408:413	arg1	Ag-NPs					430:435	Ag-NPs	430:435	Ag-NPs	430:435	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	2	74	theme	silver	408:413	arg1	nanoparticles					415:427	the silver nanoparticles	404:427	the silver nanoparticles (Ag-NPs)	404:436	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	4	75	theme	antibacterial	1397:1409	arg1	effect					1411:1416	long-term antibacterial effect	1387:1416	long-term antibacterial effect	1387:1416	In summary, under the dual regulations of the strong adhesion of PDA and CS chelation, the "inside-outside" Ag-NPs-loaded composite fiber was endowed with good physiological stability, long-term antibacterial effect and bone infection inhibition ability, making it a promising bone implant material.
33232699	0	76	theme	polylactic	39:48	arg1	fiber					67:71	polylactic acid electrospun fiber	39:71	polylactic acid electrospun fiber	39:71	Inside-outside Ag nanoparticles-loaded polylactic acid electrospun fiber for long-term antibacterial and bone regeneration.
33232699	1	77	theme	Bone	124:127	arg1	infections					129:138	Bone infections	124:138	Bone infections caused by bacteria during bone graft implantations	124:189	Bone infections caused by bacteria during bone graft implantations can impair the ability of bone tissue repair, which is currently a clinical problem.
33232699	2	78	theme	Ag-NPs	808:813	arg1	release					797:803	the steady and slow release	777:803	the steady and slow release of Ag-NPs	777:813	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	2	79	theme	electrochemical	683:697	arg1	deposition					699:708	pulse electrochemical deposition	677:708	pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability	677:1029	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	0	80	theme	electrospun	55:65	arg1	fiber					67:71	polylactic acid electrospun fiber	39:71	polylactic acid electrospun fiber	39:71	Inside-outside Ag nanoparticles-loaded polylactic acid electrospun fiber for long-term antibacterial and bone regeneration.
33232699	2	81	used	used	640:643	arg2	chitosan					622:629	chitosan	622:629	chitosan (CS)	622:634	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	2	81	used	used	640:643	arg2	CS					632:633	CS	632:633	CS	632:633	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	2	82	theme	effect	977:982	arg1	functions					937:945	dual functions	932:945	dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability	932:1029	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	2	83	theme	composite	378:386	arg1	fiber					388:392	a polylactic acid (PLLA)/silver (Ag) composite fiber	341:392	a polylactic acid (PLLA)/silver (Ag) composite fiber	341:392	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	2	84	theme	long-lasting	950:961	arg1	effect					977:982	long-lasting antibacterial effect	950:982	long-lasting antibacterial effect	950:982	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	3	85	theme	study	1036:1040	arg1	results					1042:1048	The study results	1032:1048	The study results	1032:1048	The study results showed that the composite fiber has an excellent antibacterial effect against E. coli and S. aureus, and good osteoinductive and angiogenic properties.
33232699	2	86	theme	bone	995:998	arg1	ability					1023:1029	bone regeneration promotion ability	995:1029	bone regeneration promotion ability	995:1029	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	2	87	used	used	325:328	arg2	technique					311:319	the electrospinning technique	291:319	the electrospinning technique	291:319	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
33232699	2	88	theme	PLLA	360:363	arg1	fiber					388:392	a polylactic acid (PLLA)/silver (Ag) composite fiber	341:392	a polylactic acid (PLLA)/silver (Ag) composite fiber	341:392	In this study, the electrospinning technique was used to prepare a polylactic acid (PLLA)/silver (Ag) composite fiber, in which the silver nanoparticles (Ag-NPs) were uniformly distributed on the inner surface of PLLA fibers; dopamine (DA) was self-polymerized on the composite fiber surface to construct the adhesive polydopamine (PDA) film and chitosan (CS) was used to regulate Ag+ in situ through pulse electrochemical deposition for the construction of a stable Ag-NPs coating (CS/Ag), achieving the steady and slow release of Ag-NPs, therefore accomplishing the construction of a "inside-outside" Ag-NPs-loaded PLLA/Ag@PDA@CS/Ag composite fiber with dual functions of long-lasting antibacterial effect as well as bone regeneration promotion ability.
34934001	4	0	link	monocyte-derived	541:556	arg1	MDMs					571:574	MDMs	571:574	MDMs	571:574	In the present study, we analyzed human monocyte-derived macrophages (MDMs) using RM, revealing that macrophage activation by lipopolysaccharides (LPS), interferons (IFN), or cytokines can be identified by lipid composition, which significantly differs in M0 (resting), M1 (IFN-γ/LPS), M2a (IL-4/IL-13), and M2c (IL-10) MDMs.
34934001	4	0	link	monocyte-derived	541:556	arg1	macrophages					558:568	human monocyte-derived macrophages	535:568	human monocyte-derived macrophages (MDMs)	535:575	In the present study, we analyzed human monocyte-derived macrophages (MDMs) using RM, revealing that macrophage activation by lipopolysaccharides (LPS), interferons (IFN), or cytokines can be identified by lipid composition, which significantly differs in M0 (resting), M1 (IFN-γ/LPS), M2a (IL-4/IL-13), and M2c (IL-10) MDMs.
34934001	6	1	from	changes	1057:1063	arg1	biochemistry					1072:1083	MDM biochemistry	1068:1083	MDM biochemistry	1068:1083	We detected surface topography-induced changes in MDM biochemistry and lipid composition that were not shown by less sensitive standard methods such as cytokine expression or surface antigen analysis.
34934001	6	1	from	changes	1057:1063	arg1	composition					1095:1105	lipid composition	1089:1105	lipid composition	1089:1105	We detected surface topography-induced changes in MDM biochemistry and lipid composition that were not shown by less sensitive standard methods such as cytokine expression or surface antigen analysis.
34934001	6	2	theme	topography-induced	1038:1055	arg1	changes					1057:1063	surface topography-induced changes	1030:1063	surface topography-induced changes in MDM biochemistry and lipid composition that were not shown by less sensitive standard methods such as cytokine expression or surface antigen analysis	1030:1216	We detected surface topography-induced changes in MDM biochemistry and lipid composition that were not shown by less sensitive standard methods such as cytokine expression or surface antigen analysis.
34934001	4	3	theme	human	535:539	arg1	MDMs					571:574	MDMs	571:574	MDMs	571:574	In the present study, we analyzed human monocyte-derived macrophages (MDMs) using RM, revealing that macrophage activation by lipopolysaccharides (LPS), interferons (IFN), or cytokines can be identified by lipid composition, which significantly differs in M0 (resting), M1 (IFN-γ/LPS), M2a (IL-4/IL-13), and M2c (IL-10) MDMs.
34934001	4	3	theme	human	535:539	arg1	macrophages					558:568	human monocyte-derived macrophages	535:568	human monocyte-derived macrophages (MDMs)	535:575	In the present study, we analyzed human monocyte-derived macrophages (MDMs) using RM, revealing that macrophage activation by lipopolysaccharides (LPS), interferons (IFN), or cytokines can be identified by lipid composition, which significantly differs in M0 (resting), M1 (IFN-γ/LPS), M2a (IL-4/IL-13), and M2c (IL-10) MDMs.
34934001	5	4	from	impact	843:848	arg1	polarization					888:899	polarization	888:899	polarization	888:899	To identify the impact of a biomaterial on MDM phenotype and polarization, we cultured macrophages on titanium disks with varying surface topographies and analyzed the adherent MDMs with RM.
34934001	5	4	from	impact	843:848	arg1	phenotype					874:882	MDM phenotype	870:882	MDM phenotype	870:882	To identify the impact of a biomaterial on MDM phenotype and polarization, we cultured macrophages on titanium disks with varying surface topographies and analyzed the adherent MDMs with RM.
34934001	4	5	theme	lipid	707:711	arg1	composition					713:723	lipid composition	707:723	lipid composition	707:723	In the present study, we analyzed human monocyte-derived macrophages (MDMs) using RM, revealing that macrophage activation by lipopolysaccharides (LPS), interferons (IFN), or cytokines can be identified by lipid composition, which significantly differs in M0 (resting), M1 (IFN-γ/LPS), M2a (IL-4/IL-13), and M2c (IL-10) MDMs.
34934001	0	6	theme	materials	114:122	arg1	topographies					90:101	surface topographies	82:101	surface topographies of implant materials	82:122	Lipidome profiling with Raman microspectroscopy identifies macrophage response to surface topographies of implant materials.
34934001	7	7	theme	interaction	1337:1347	arg1	classification					1270:1283	a more precise classification	1255:1283	a more precise classification of macrophage activation and biomaterial-macrophage interaction	1255:1347	Our data suggest that RM may enable a more precise classification of macrophage activation and biomaterial-macrophage interaction.
34934001	6	8	theme	sensitive	1135:1143	arg1	expression					1179:1188	cytokine expression	1170:1188	cytokine expression	1170:1188	We detected surface topography-induced changes in MDM biochemistry and lipid composition that were not shown by less sensitive standard methods such as cytokine expression or surface antigen analysis.
34934001	6	8	theme	sensitive	1135:1143	arg1	methods					1154:1160	less sensitive standard methods	1130:1160	less sensitive standard methods such as cytokine expression or surface antigen analysis	1130:1216	We detected surface topography-induced changes in MDM biochemistry and lipid composition that were not shown by less sensitive standard methods such as cytokine expression or surface antigen analysis.
34934001	6	8	theme	sensitive	1135:1143	arg1	analysis					1209:1216	surface antigen analysis	1193:1216	surface antigen analysis	1193:1216	We detected surface topography-induced changes in MDM biochemistry and lipid composition that were not shown by less sensitive standard methods such as cytokine expression or surface antigen analysis.
34934001	0	9	theme	implant	106:112	arg1	materials					114:122	implant materials	106:122	implant materials	106:122	Lipidome profiling with Raman microspectroscopy identifies macrophage response to surface topographies of implant materials.
34934001	5	10	theme	MDM	870:872	arg1	phenotype					874:882	MDM phenotype	870:882	MDM phenotype	870:882	To identify the impact of a biomaterial on MDM phenotype and polarization, we cultured macrophages on titanium disks with varying surface topographies and analyzed the adherent MDMs with RM.
34934001	7	11	theme	precise	1262:1268	arg1	classification					1270:1283	a more precise classification	1255:1283	a more precise classification of macrophage activation and biomaterial-macrophage interaction	1255:1347	Our data suggest that RM may enable a more precise classification of macrophage activation and biomaterial-macrophage interaction.
34934001	3	12	theme	living	443:448	arg1	cells					450:454	living cells	443:454	living cells	443:454	Raman microspectroscopy (RM) is a marker-independent, noninvasive technology that allows the analysis of living cells without the need for staining or processing.
34934001	6	13	theme	surface	1030:1036	arg1	changes					1057:1063	surface topography-induced changes	1030:1063	surface topography-induced changes in MDM biochemistry and lipid composition that were not shown by less sensitive standard methods such as cytokine expression or surface antigen analysis	1030:1216	We detected surface topography-induced changes in MDM biochemistry and lipid composition that were not shown by less sensitive standard methods such as cytokine expression or surface antigen analysis.
34934001	7	14	theme	macrophage	1288:1297	arg1	activation					1299:1308	macrophage activation	1288:1308	macrophage activation	1288:1308	Our data suggest that RM may enable a more precise classification of macrophage activation and biomaterial-macrophage interaction.
34934001	5	15	with	disks	938:942	arg1	topographies					965:976	varying surface topographies	949:976	varying surface topographies	949:976	To identify the impact of a biomaterial on MDM phenotype and polarization, we cultured macrophages on titanium disks with varying surface topographies and analyzed the adherent MDMs with RM.
34934001	0	16	theme	surface	82:88	arg1	topographies					90:101	surface topographies	82:101	surface topographies of implant materials	82:122	Lipidome profiling with Raman microspectroscopy identifies macrophage response to surface topographies of implant materials.
34934001	1	17	theme	macrophage	210:219	arg1	phenotypes					221:230	macrophage phenotypes	210:230	macrophage phenotypes	210:230	Biomaterial characteristics such as surface topographies have been shown to modulate macrophage phenotypes.
34934001	0	18	theme	Lipidome	0:7	arg1	profiling					9:17	Lipidome profiling	0:17	Lipidome profiling with Raman microspectroscopy	0:46	Lipidome profiling with Raman microspectroscopy identifies macrophage response to surface topographies of implant materials.
34934001	6	19	theme	antigen	1201:1207	arg1	analysis					1209:1216	surface antigen analysis	1193:1216	surface antigen analysis	1193:1216	We detected surface topography-induced changes in MDM biochemistry and lipid composition that were not shown by less sensitive standard methods such as cytokine expression or surface antigen analysis.
34934001	0	20	theme	Raman	24:28	arg1	microspectroscopy					30:46	Raman microspectroscopy	24:46	Raman microspectroscopy	24:46	Lipidome profiling with Raman microspectroscopy identifies macrophage response to surface topographies of implant materials.
34934001	6	21	theme	cytokine	1170:1177	arg1	expression					1179:1188	cytokine expression	1170:1188	cytokine expression	1170:1188	We detected surface topography-induced changes in MDM biochemistry and lipid composition that were not shown by less sensitive standard methods such as cytokine expression or surface antigen analysis.
34934001	6	22	theme	surface	1193:1199	arg1	analysis					1209:1216	surface antigen analysis	1193:1216	surface antigen analysis	1193:1216	We detected surface topography-induced changes in MDM biochemistry and lipid composition that were not shown by less sensitive standard methods such as cytokine expression or surface antigen analysis.
34934001	6	23	theme	MDM	1068:1070	arg1	biochemistry					1072:1083	MDM biochemistry	1068:1083	MDM biochemistry	1068:1083	We detected surface topography-induced changes in MDM biochemistry and lipid composition that were not shown by less sensitive standard methods such as cytokine expression or surface antigen analysis.
34934001	4	24	theme	macrophage	602:611	arg1	activation					613:622	macrophage activation	602:622	macrophage activation by lipopolysaccharides (LPS), interferons (IFN), or cytokines	602:684	In the present study, we analyzed human monocyte-derived macrophages (MDMs) using RM, revealing that macrophage activation by lipopolysaccharides (LPS), interferons (IFN), or cytokines can be identified by lipid composition, which significantly differs in M0 (resting), M1 (IFN-γ/LPS), M2a (IL-4/IL-13), and M2c (IL-10) MDMs.
34934001	3	25	theme	Raman	338:342	arg1	technology					404:413	a marker-independent, noninvasive technology	370:413	a marker-independent, noninvasive technology that allows the analysis of living cells without the need for staining or processing	370:498	Raman microspectroscopy (RM) is a marker-independent, noninvasive technology that allows the analysis of living cells without the need for staining or processing.
34934001	3	25	theme	Raman	338:342	arg1	RM					363:364	RM	363:364	RM	363:364	Raman microspectroscopy (RM) is a marker-independent, noninvasive technology that allows the analysis of living cells without the need for staining or processing.
34934001	3	25	theme	Raman	338:342	arg1	microspectroscopy					344:360	Raman microspectroscopy	338:360	Raman microspectroscopy (RM)	338:365	Raman microspectroscopy (RM) is a marker-independent, noninvasive technology that allows the analysis of living cells without the need for staining or processing.
34934001	1	26	theme	Biomaterial	125:135	arg1	characteristics					137:151	Biomaterial characteristics	125:151	Biomaterial characteristics such as surface topographies	125:180	Biomaterial characteristics such as surface topographies have been shown to modulate macrophage phenotypes.
34934001	1	26	theme	Biomaterial	125:135	arg1	topographies					169:180	surface topographies	161:180	surface topographies	161:180	Biomaterial characteristics such as surface topographies have been shown to modulate macrophage phenotypes.
34934001	3	27	dep	marker-independent	372:389	arg1	noninvasive					392:402	noninvasive	392:402	noninvasive	392:402	Raman microspectroscopy (RM) is a marker-independent, noninvasive technology that allows the analysis of living cells without the need for staining or processing.
34934001	5	28	theme	surface	957:963	arg1	topographies					965:976	varying surface topographies	949:976	varying surface topographies	949:976	To identify the impact of a biomaterial on MDM phenotype and polarization, we cultured macrophages on titanium disks with varying surface topographies and analyzed the adherent MDMs with RM.
34934001	4	29	theme	present	508:514	arg1	study					516:520	the present study	504:520	the present study	504:520	In the present study, we analyzed human monocyte-derived macrophages (MDMs) using RM, revealing that macrophage activation by lipopolysaccharides (LPS), interferons (IFN), or cytokines can be identified by lipid composition, which significantly differs in M0 (resting), M1 (IFN-γ/LPS), M2a (IL-4/IL-13), and M2c (IL-10) MDMs.
34934001	2	30	theme	macrophage	271:280	arg1	response					282:289	macrophage response	271:289	macrophage response to biomaterials	271:305	The standard methodologies to measure macrophage response to biomaterials are marker-based and invasive.
34934001	3	31	theme	cells	450:454	arg1	analysis					431:438	the analysis	427:438	the analysis of living cells	427:454	Raman microspectroscopy (RM) is a marker-independent, noninvasive technology that allows the analysis of living cells without the need for staining or processing.
34934001	6	32	theme	lipid	1089:1093	arg1	composition					1095:1105	lipid composition	1089:1105	lipid composition	1089:1105	We detected surface topography-induced changes in MDM biochemistry and lipid composition that were not shown by less sensitive standard methods such as cytokine expression or surface antigen analysis.
34934001	0	33	theme	macrophage	59:68	arg1	response					70:77	macrophage response	59:77	macrophage response to surface topographies of implant materials	59:122	Lipidome profiling with Raman microspectroscopy identifies macrophage response to surface topographies of implant materials.
34934001	5	34	theme	adherent	995:1002	arg1	MDMs					1004:1007	the adherent MDMs	991:1007	the adherent MDMs	991:1007	To identify the impact of a biomaterial on MDM phenotype and polarization, we cultured macrophages on titanium disks with varying surface topographies and analyzed the adherent MDMs with RM.
34934001	0	35	with	profiling	9:17	arg1	microspectroscopy					30:46	Raman microspectroscopy	24:46	Raman microspectroscopy	24:46	Lipidome profiling with Raman microspectroscopy identifies macrophage response to surface topographies of implant materials.
34934001	5	36	theme	biomaterial	855:865	arg1	impact					843:848	the impact	839:848	the impact of a biomaterial on MDM phenotype and polarization	839:899	To identify the impact of a biomaterial on MDM phenotype and polarization, we cultured macrophages on titanium disks with varying surface topographies and analyzed the adherent MDMs with RM.
34934001	6	37	theme	standard	1145:1152	arg1	expression					1179:1188	cytokine expression	1170:1188	cytokine expression	1170:1188	We detected surface topography-induced changes in MDM biochemistry and lipid composition that were not shown by less sensitive standard methods such as cytokine expression or surface antigen analysis.
34934001	6	37	theme	standard	1145:1152	arg1	methods					1154:1160	less sensitive standard methods	1130:1160	less sensitive standard methods such as cytokine expression or surface antigen analysis	1130:1216	We detected surface topography-induced changes in MDM biochemistry and lipid composition that were not shown by less sensitive standard methods such as cytokine expression or surface antigen analysis.
34934001	6	37	theme	standard	1145:1152	arg1	analysis					1209:1216	surface antigen analysis	1193:1216	surface antigen analysis	1193:1216	We detected surface topography-induced changes in MDM biochemistry and lipid composition that were not shown by less sensitive standard methods such as cytokine expression or surface antigen analysis.
34934001	2	38	theme	standard	237:244	arg1	marker-based					311:322	marker-based	311:322	marker-based	311:322	The standard methodologies to measure macrophage response to biomaterials are marker-based and invasive.
34934001	2	38	theme	standard	237:244	arg1	methodologies					246:258	The standard methodologies	233:258	The standard methodologies to measure macrophage response to biomaterials	233:305	The standard methodologies to measure macrophage response to biomaterials are marker-based and invasive.
34934001	5	39	theme	titanium	929:936	arg1	disks					938:942	titanium disks	929:942	titanium disks with varying surface topographies	929:976	To identify the impact of a biomaterial on MDM phenotype and polarization, we cultured macrophages on titanium disks with varying surface topographies and analyzed the adherent MDMs with RM.
34934001	4	40	dep	M0	757:758	arg1	MDMs					821:824	MDMs	821:824	MDMs	821:824	In the present study, we analyzed human monocyte-derived macrophages (MDMs) using RM, revealing that macrophage activation by lipopolysaccharides (LPS), interferons (IFN), or cytokines can be identified by lipid composition, which significantly differs in M0 (resting), M1 (IFN-γ/LPS), M2a (IL-4/IL-13), and M2c (IL-10) MDMs.
34934001	7	41	theme	biomaterial-macrophage	1314:1335	arg1	interaction					1337:1347	biomaterial-macrophage interaction	1314:1347	biomaterial-macrophage interaction	1314:1347	Our data suggest that RM may enable a more precise classification of macrophage activation and biomaterial-macrophage interaction.
34934001	1	42	theme	surface	161:167	arg1	topographies					169:180	surface topographies	161:180	surface topographies	161:180	Biomaterial characteristics such as surface topographies have been shown to modulate macrophage phenotypes.
34934001	3	43	theme	marker-independent	372:389	arg1	technology					404:413	a marker-independent, noninvasive technology	370:413	a marker-independent, noninvasive technology that allows the analysis of living cells without the need for staining or processing	370:498	Raman microspectroscopy (RM) is a marker-independent, noninvasive technology that allows the analysis of living cells without the need for staining or processing.
34934001	3	43	theme	marker-independent	372:389	arg1	microspectroscopy					344:360	Raman microspectroscopy	338:360	Raman microspectroscopy (RM)	338:365	Raman microspectroscopy (RM) is a marker-independent, noninvasive technology that allows the analysis of living cells without the need for staining or processing.
34934001	7	44	theme	activation	1299:1308	arg1	classification					1270:1283	a more precise classification	1255:1283	a more precise classification of macrophage activation and biomaterial-macrophage interaction	1255:1347	Our data suggest that RM may enable a more precise classification of macrophage activation and biomaterial-macrophage interaction.
34934001	2	45	dep	methodologies	246:258	arg1	measure					263:269	measure	263:269	to measure macrophage response to biomaterials	260:305	The standard methodologies to measure macrophage response to biomaterials are marker-based and invasive.
34934001	4	46	theme	monocyte-derived	541:556	arg1	MDMs					571:574	MDMs	571:574	MDMs	571:574	In the present study, we analyzed human monocyte-derived macrophages (MDMs) using RM, revealing that macrophage activation by lipopolysaccharides (LPS), interferons (IFN), or cytokines can be identified by lipid composition, which significantly differs in M0 (resting), M1 (IFN-γ/LPS), M2a (IL-4/IL-13), and M2c (IL-10) MDMs.
34934001	4	46	theme	monocyte-derived	541:556	arg1	macrophages					558:568	human monocyte-derived macrophages	535:568	human monocyte-derived macrophages (MDMs)	535:575	In the present study, we analyzed human monocyte-derived macrophages (MDMs) using RM, revealing that macrophage activation by lipopolysaccharides (LPS), interferons (IFN), or cytokines can be identified by lipid composition, which significantly differs in M0 (resting), M1 (IFN-γ/LPS), M2a (IL-4/IL-13), and M2c (IL-10) MDMs.
34934001	5	47	theme	varying	949:955	arg1	topographies					965:976	varying surface topographies	949:976	varying surface topographies	949:976	To identify the impact of a biomaterial on MDM phenotype and polarization, we cultured macrophages on titanium disks with varying surface topographies and analyzed the adherent MDMs with RM.
32648191	7	0	theme	advanced	1499:1506	arg1	OA					1508:1509	advanced OA	1499:1509	advanced OA	1499:1509	These findings suggest different mechanisms contribute to cartilage viscosity in low and high frequencies, and that the loss of superficial collagen pretension during early OA is due to lower tissue swelling (PG loss), while in advanced OA, both collagen disorganization and lower swelling modulate the collagen fibril pretension.
32648191	1	1	theme	functional	199:208	arg1	properties					210:219	constituent-specific functional properties	178:219	constituent-specific functional properties	178:219	Relationships between composition, structure and constituent-specific functional properties of human articular cartilage at different stages of osteoarthritis (OA) are poorly known.
32648191	7	2	theme	pretension	1420:1429	arg1	loss					1391:1394	the loss	1387:1394	the loss of superficial collagen pretension during early OA	1387:1445	These findings suggest different mechanisms contribute to cartilage viscosity in low and high frequencies, and that the loss of superficial collagen pretension during early OA is due to lower tissue swelling (PG loss), while in advanced OA, both collagen disorganization and lower swelling modulate the collagen fibril pretension.
32648191	7	2	theme	pretension	1420:1429	arg1	due					1450:1452	due	1450:1452	due	1450:1452	These findings suggest different mechanisms contribute to cartilage viscosity in low and high frequencies, and that the loss of superficial collagen pretension during early OA is due to lower tissue swelling (PG loss), while in advanced OA, both collagen disorganization and lower swelling modulate the collagen fibril pretension.
32648191	2	3	with	comparison	349:358	arg1	analysis					476:483	microscopic and spectroscopic analysis	446:483	analysis	476:483	We established these relationships by comparison of elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties with microscopic and spectroscopic analysis of structure and composition of healthy and osteoarthritic human tibial cartilage (n = 27).
32648191	0	4	theme	Experimental	87:98	arg1	Investigation					114:126	Experimental and Numerical Investigation	87:126	Experimental and Numerical Investigation	87:126	Structure-Function Relationships of Healthy and Osteoarthritic Human Tibial Cartilage: Experimental and Numerical Investigation.
32648191	5	5	theme	healthy	984:990	arg1	group					992:996	the healthy group	980:996	the healthy group	980:996	Proteoglycans were lost in the early and advanced OA groups compared to the healthy group, while the superficial collagen orientation angle was greater only in the advanced OA group compared to the healthy group.
32648191	2	6	theme	poroelastic	407:417	arg1	properties					430:439	elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties	363:439	elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties	363:439	We established these relationships by comparison of elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties with microscopic and spectroscopic analysis of structure and composition of healthy and osteoarthritic human tibial cartilage (n = 27).
32648191	7	7	theme	fibril	1583:1588	arg1	pretension					1590:1599	the collagen fibril pretension	1570:1599	the collagen fibril pretension	1570:1599	These findings suggest different mechanisms contribute to cartilage viscosity in low and high frequencies, and that the loss of superficial collagen pretension during early OA is due to lower tissue swelling (PG loss), while in advanced OA, both collagen disorganization and lower swelling modulate the collagen fibril pretension.
32648191	6	8	theme	fibril	1173:1178	arg1	pretension					1180:1189	fibril pretension	1173:1189	fibril pretension	1173:1189	Simultaneously, the initial fibril network modulus (fibril pretension) was smaller in the early and advanced OA groups compared to the healthy group.
32648191	6	8	theme	fibril	1173:1178	arg1	modulus					1164:1170	the initial fibril network modulus	1137:1170	the initial fibril network modulus (fibril pretension)	1137:1190	Simultaneously, the initial fibril network modulus (fibril pretension) was smaller in the early and advanced OA groups compared to the healthy group.
32648191	7	9	theme	early	1438:1442	arg1	OA					1444:1445	early OA	1438:1445	early OA	1438:1445	These findings suggest different mechanisms contribute to cartilage viscosity in low and high frequencies, and that the loss of superficial collagen pretension during early OA is due to lower tissue swelling (PG loss), while in advanced OA, both collagen disorganization and lower swelling modulate the collagen fibril pretension.
32648191	5	10	theme	healthy	1106:1112	arg1	group					1114:1118	the healthy group	1102:1118	the healthy group	1102:1118	Proteoglycans were lost in the early and advanced OA groups compared to the healthy group, while the superficial collagen orientation angle was greater only in the advanced OA group compared to the healthy group.
32648191	6	11	theme	early	1211:1215	arg1	groups					1233:1238	the early and advanced OA groups	1207:1238	the early and advanced OA groups	1207:1238	Simultaneously, the initial fibril network modulus (fibril pretension) was smaller in the early and advanced OA groups compared to the healthy group.
32648191	0	12	theme	Numerical	104:112	arg1	Investigation					114:126	Experimental and Numerical Investigation	87:126	Experimental and Numerical Investigation	87:126	Structure-Function Relationships of Healthy and Osteoarthritic Human Tibial Cartilage: Experimental and Numerical Investigation.
32648191	6	13	theme	advanced	1221:1228	arg1	groups					1233:1238	the early and advanced OA groups	1207:1238	the early and advanced OA groups	1207:1238	Simultaneously, the initial fibril network modulus (fibril pretension) was smaller in the early and advanced OA groups compared to the healthy group.
32648191	3	14	theme	proteoglycan	608:619	arg1	content					621:627	proteoglycan content	608:627	proteoglycan content	608:627	At a low frequency (0.005 Hz), proteoglycan content correlated negatively and collagen content correlated positively with the phase difference (i.e. tissue viscosity).
32648191	2	15	theme	mechanical	419:428	arg1	properties					430:439	elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties	363:439	elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties	363:439	We established these relationships by comparison of elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties with microscopic and spectroscopic analysis of structure and composition of healthy and osteoarthritic human tibial cartilage (n = 27).
32648191	4	16	theme	proteoglycan	785:796	arg1	content					798:804	proteoglycan content	785:804	proteoglycan content	785:804	At a high-frequency regime (> 0.05 Hz), proteoglycan content correlated negatively and collagen orientation angle correlated positively with the phase difference.
32648191	0	17	dep	Investigation	114:126	arg1	Relationships					19:31	Structure-Function Relationships	0:31	Structure-Function Relationships of Healthy and Osteoarthritic Human Tibial Cartilage	0:84	Structure-Function Relationships of Healthy and Osteoarthritic Human Tibial Cartilage: Experimental and Numerical Investigation.
32648191	4	18	theme	a	748:748	arg1	> 0.05 Hz					773:781	> 0.05 Hz	773:781	> 0.05 Hz	773:781	At a high-frequency regime (> 0.05 Hz), proteoglycan content correlated negatively and collagen orientation angle correlated positively with the phase difference.
32648191	4	18	theme	a	748:748	arg1	regime					765:770	a high-frequency regime	748:770	a high-frequency regime (> 0.05 Hz)	748:782	At a high-frequency regime (> 0.05 Hz), proteoglycan content correlated negatively and collagen orientation angle correlated positively with the phase difference.
32648191	5	19	theme	advanced	949:956	arg1	groups					961:966	the early and advanced OA groups	935:966	the early and advanced OA groups	935:966	Proteoglycans were lost in the early and advanced OA groups compared to the healthy group, while the superficial collagen orientation angle was greater only in the advanced OA group compared to the healthy group.
32648191	2	20	theme	human	544:548	arg1	n = 27					568:573	n = 27	568:573	n = 27	568:573	We established these relationships by comparison of elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties with microscopic and spectroscopic analysis of structure and composition of healthy and osteoarthritic human tibial cartilage (n = 27).
32648191	2	20	theme	human	544:548	arg1	cartilage					557:565	healthy and osteoarthritic human tibial cartilage	517:565	cartilage	557:565	We established these relationships by comparison of elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties with microscopic and spectroscopic analysis of structure and composition of healthy and osteoarthritic human tibial cartilage (n = 27).
32648191	5	21	theme	collagen	1021:1028	arg1	angle					1042:1046	the superficial collagen orientation angle	1005:1046	the superficial collagen orientation angle	1005:1046	Proteoglycans were lost in the early and advanced OA groups compared to the healthy group, while the superficial collagen orientation angle was greater only in the advanced OA group compared to the healthy group.
32648191	5	21	theme	collagen	1021:1028	arg1	greater					1052:1058	greater	1052:1058	greater	1052:1058	Proteoglycans were lost in the early and advanced OA groups compared to the healthy group, while the superficial collagen orientation angle was greater only in the advanced OA group compared to the healthy group.
32648191	1	22	theme	human	224:228	arg1	cartilage					240:248	human articular cartilage	224:248	human articular cartilage	224:248	Relationships between composition, structure and constituent-specific functional properties of human articular cartilage at different stages of osteoarthritis (OA) are poorly known.
32648191	2	23	theme	osteoarthritic	529:542	arg1	n = 27					568:573	n = 27	568:573	n = 27	568:573	We established these relationships by comparison of elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties with microscopic and spectroscopic analysis of structure and composition of healthy and osteoarthritic human tibial cartilage (n = 27).
32648191	2	23	theme	osteoarthritic	529:542	arg1	cartilage					557:565	healthy and osteoarthritic human tibial cartilage	517:565	cartilage	557:565	We established these relationships by comparison of elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties with microscopic and spectroscopic analysis of structure and composition of healthy and osteoarthritic human tibial cartilage (n = 27).
32648191	6	24	theme	OA	1230:1231	arg1	groups					1233:1238	the early and advanced OA groups	1207:1238	the early and advanced OA groups	1207:1238	Simultaneously, the initial fibril network modulus (fibril pretension) was smaller in the early and advanced OA groups compared to the healthy group.
32648191	2	25	theme	elastic	363:369	arg1	properties					430:439	elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties	363:439	elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties	363:439	We established these relationships by comparison of elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties with microscopic and spectroscopic analysis of structure and composition of healthy and osteoarthritic human tibial cartilage (n = 27).
32648191	7	26	theme	low	1352:1354	arg1	frequencies					1365:1375	low and high frequencies	1352:1375	low and high frequencies	1352:1375	These findings suggest different mechanisms contribute to cartilage viscosity in low and high frequencies, and that the loss of superficial collagen pretension during early OA is due to lower tissue swelling (PG loss), while in advanced OA, both collagen disorganization and lower swelling modulate the collagen fibril pretension.
32648191	1	27	theme	articular	230:238	arg1	cartilage					240:248	human articular cartilage	224:248	human articular cartilage	224:248	Relationships between composition, structure and constituent-specific functional properties of human articular cartilage at different stages of osteoarthritis (OA) are poorly known.
32648191	0	28	theme	Structure-Function	0:17	arg1	Relationships					19:31	Structure-Function Relationships	0:31	Structure-Function Relationships of Healthy and Osteoarthritic Human Tibial Cartilage	0:84	Structure-Function Relationships of Healthy and Osteoarthritic Human Tibial Cartilage: Experimental and Numerical Investigation.
32648191	5	29	theme	OA	958:959	arg1	groups					961:966	the early and advanced OA groups	935:966	the early and advanced OA groups	935:966	Proteoglycans were lost in the early and advanced OA groups compared to the healthy group, while the superficial collagen orientation angle was greater only in the advanced OA group compared to the healthy group.
32648191	1	30	theme	cartilage	240:248	arg1	structure					164:172	structure	164:172	structure	164:172	Relationships between composition, structure and constituent-specific functional properties of human articular cartilage at different stages of osteoarthritis (OA) are poorly known.
32648191	1	30	theme	cartilage	240:248	arg1	composition					151:161	composition	151:161	composition	151:161	Relationships between composition, structure and constituent-specific functional properties of human articular cartilage at different stages of osteoarthritis (OA) are poorly known.
32648191	1	30	theme	cartilage	240:248	arg1	properties					210:219	constituent-specific functional properties	178:219	constituent-specific functional properties	178:219	Relationships between composition, structure and constituent-specific functional properties of human articular cartilage at different stages of osteoarthritis (OA) are poorly known.
32648191	0	31	theme	Healthy	36:42	arg1	Cartilage					76:84	Healthy and Osteoarthritic Human Tibial Cartilage	36:84	Healthy and Osteoarthritic Human Tibial Cartilage	36:84	Structure-Function Relationships of Healthy and Osteoarthritic Human Tibial Cartilage: Experimental and Numerical Investigation.
32648191	6	32	theme	network	1156:1162	arg1	pretension					1180:1189	fibril pretension	1173:1189	fibril pretension	1173:1189	Simultaneously, the initial fibril network modulus (fibril pretension) was smaller in the early and advanced OA groups compared to the healthy group.
32648191	6	32	theme	network	1156:1162	arg1	smaller					1196:1202	smaller	1196:1202	smaller	1196:1202	Simultaneously, the initial fibril network modulus (fibril pretension) was smaller in the early and advanced OA groups compared to the healthy group.
32648191	6	32	theme	network	1156:1162	arg1	modulus					1164:1170	the initial fibril network modulus	1137:1170	the initial fibril network modulus (fibril pretension)	1137:1190	Simultaneously, the initial fibril network modulus (fibril pretension) was smaller in the early and advanced OA groups compared to the healthy group.
32648191	5	33	theme	superficial	1009:1019	arg1	angle					1042:1046	the superficial collagen orientation angle	1005:1046	the superficial collagen orientation angle	1005:1046	Proteoglycans were lost in the early and advanced OA groups compared to the healthy group, while the superficial collagen orientation angle was greater only in the advanced OA group compared to the healthy group.
32648191	5	33	theme	superficial	1009:1019	arg1	greater					1052:1058	greater	1052:1058	greater	1052:1058	Proteoglycans were lost in the early and advanced OA groups compared to the healthy group, while the superficial collagen orientation angle was greater only in the advanced OA group compared to the healthy group.
32648191	4	34	theme	collagen	832:839	arg1	angle					853:857	collagen orientation angle	832:857	collagen orientation angle	832:857	At a high-frequency regime (> 0.05 Hz), proteoglycan content correlated negatively and collagen orientation angle correlated positively with the phase difference.
32648191	2	35	theme	composition	502:512	arg1	analysis					476:483	microscopic and spectroscopic analysis	446:483	analysis	476:483	We established these relationships by comparison of elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties with microscopic and spectroscopic analysis of structure and composition of healthy and osteoarthritic human tibial cartilage (n = 27).
32648191	5	36	theme	orientation	1030:1040	arg1	angle					1042:1046	the superficial collagen orientation angle	1005:1046	the superficial collagen orientation angle	1005:1046	Proteoglycans were lost in the early and advanced OA groups compared to the healthy group, while the superficial collagen orientation angle was greater only in the advanced OA group compared to the healthy group.
32648191	5	36	theme	orientation	1030:1040	arg1	greater					1052:1058	greater	1052:1058	greater	1052:1058	Proteoglycans were lost in the early and advanced OA groups compared to the healthy group, while the superficial collagen orientation angle was greater only in the advanced OA group compared to the healthy group.
32648191	4	37	dep	regime	765:770	arg1	high-frequency					750:763	high-frequency	750:763	high-frequency	750:763	At a high-frequency regime (> 0.05 Hz), proteoglycan content correlated negatively and collagen orientation angle correlated positively with the phase difference.
32648191	3	38	theme	tissue	726:731	arg1	viscosity					733:741	tissue viscosity	726:741	tissue viscosity	726:741	At a low frequency (0.005 Hz), proteoglycan content correlated negatively and collagen content correlated positively with the phase difference (i.e. tissue viscosity).
32648191	3	39	theme	low	582:584	arg1	0.005 Hz					597:604	0.005 Hz	597:604	0.005 Hz	597:604	At a low frequency (0.005 Hz), proteoglycan content correlated negatively and collagen content correlated positively with the phase difference (i.e. tissue viscosity).
32648191	3	39	theme	low	582:584	arg1	frequency					586:594	a low frequency	580:594	a low frequency (0.005 Hz)	580:605	At a low frequency (0.005 Hz), proteoglycan content correlated negatively and collagen content correlated positively with the phase difference (i.e. tissue viscosity).
32648191	2	40	theme	tibial	550:555	arg1	n = 27					568:573	n = 27	568:573	n = 27	568:573	We established these relationships by comparison of elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties with microscopic and spectroscopic analysis of structure and composition of healthy and osteoarthritic human tibial cartilage (n = 27).
32648191	2	40	theme	tibial	550:555	arg1	cartilage					557:565	healthy and osteoarthritic human tibial cartilage	517:565	cartilage	557:565	We established these relationships by comparison of elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties with microscopic and spectroscopic analysis of structure and composition of healthy and osteoarthritic human tibial cartilage (n = 27).
32648191	1	41	theme	different	253:261	arg1	stages					263:268	different stages	253:268	different stages of osteoarthritis (OA)	253:291	Relationships between composition, structure and constituent-specific functional properties of human articular cartilage at different stages of osteoarthritis (OA) are poorly known.
32648191	0	42	theme	Osteoarthritic	48:61	arg1	Cartilage					76:84	Healthy and Osteoarthritic Human Tibial Cartilage	36:84	Healthy and Osteoarthritic Human Tibial Cartilage	36:84	Structure-Function Relationships of Healthy and Osteoarthritic Human Tibial Cartilage: Experimental and Numerical Investigation.
32648191	5	43	theme	early	939:943	arg1	groups					961:966	the early and advanced OA groups	935:966	the early and advanced OA groups	935:966	Proteoglycans were lost in the early and advanced OA groups compared to the healthy group, while the superficial collagen orientation angle was greater only in the advanced OA group compared to the healthy group.
32648191	2	44	theme	microscopic	446:456	arg1	analysis					476:483	microscopic and spectroscopic analysis	446:483	analysis	476:483	We established these relationships by comparison of elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties with microscopic and spectroscopic analysis of structure and composition of healthy and osteoarthritic human tibial cartilage (n = 27).
32648191	2	45	theme	structure	488:496	arg1	analysis					476:483	microscopic and spectroscopic analysis	446:483	analysis	476:483	We established these relationships by comparison of elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties with microscopic and spectroscopic analysis of structure and composition of healthy and osteoarthritic human tibial cartilage (n = 27).
32648191	2	46	theme	cartilage	557:565	arg1	structure					488:496	structure	488:496	structure	488:496	We established these relationships by comparison of elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties with microscopic and spectroscopic analysis of structure and composition of healthy and osteoarthritic human tibial cartilage (n = 27).
32648191	2	46	theme	cartilage	557:565	arg1	composition					502:512	composition	502:512	composition	502:512	We established these relationships by comparison of elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties with microscopic and spectroscopic analysis of structure and composition of healthy and osteoarthritic human tibial cartilage (n = 27).
32648191	4	47	theme	orientation	841:851	arg1	angle					853:857	collagen orientation angle	832:857	collagen orientation angle	832:857	At a high-frequency regime (> 0.05 Hz), proteoglycan content correlated negatively and collagen orientation angle correlated positively with the phase difference.
32648191	2	48	theme	healthy	517:523	arg1	n = 27					568:573	n = 27	568:573	n = 27	568:573	We established these relationships by comparison of elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties with microscopic and spectroscopic analysis of structure and composition of healthy and osteoarthritic human tibial cartilage (n = 27).
32648191	2	48	theme	healthy	517:523	arg1	cartilage					557:565	healthy and osteoarthritic human tibial cartilage	517:565	cartilage	557:565	We established these relationships by comparison of elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties with microscopic and spectroscopic analysis of structure and composition of healthy and osteoarthritic human tibial cartilage (n = 27).
32648191	1	49	from	stages	263:268	arg1	structure					164:172	structure	164:172	structure	164:172	Relationships between composition, structure and constituent-specific functional properties of human articular cartilage at different stages of osteoarthritis (OA) are poorly known.
32648191	1	49	from	stages	263:268	arg1	composition					151:161	composition	151:161	composition	151:161	Relationships between composition, structure and constituent-specific functional properties of human articular cartilage at different stages of osteoarthritis (OA) are poorly known.
32648191	1	49	from	stages	263:268	arg1	properties					210:219	constituent-specific functional properties	178:219	constituent-specific functional properties	178:219	Relationships between composition, structure and constituent-specific functional properties of human articular cartilage at different stages of osteoarthritis (OA) are poorly known.
32648191	6	50	theme	fibril	1149:1154	arg1	pretension					1180:1189	fibril pretension	1173:1189	fibril pretension	1173:1189	Simultaneously, the initial fibril network modulus (fibril pretension) was smaller in the early and advanced OA groups compared to the healthy group.
32648191	6	50	theme	fibril	1149:1154	arg1	smaller					1196:1202	smaller	1196:1202	smaller	1196:1202	Simultaneously, the initial fibril network modulus (fibril pretension) was smaller in the early and advanced OA groups compared to the healthy group.
32648191	6	50	theme	fibril	1149:1154	arg1	modulus					1164:1170	the initial fibril network modulus	1137:1170	the initial fibril network modulus (fibril pretension)	1137:1190	Simultaneously, the initial fibril network modulus (fibril pretension) was smaller in the early and advanced OA groups compared to the healthy group.
32648191	0	51	theme	Tibial	69:74	arg1	Cartilage					76:84	Healthy and Osteoarthritic Human Tibial Cartilage	36:84	Healthy and Osteoarthritic Human Tibial Cartilage	36:84	Structure-Function Relationships of Healthy and Osteoarthritic Human Tibial Cartilage: Experimental and Numerical Investigation.
32648191	7	52	theme	collagen	1574:1581	arg1	pretension					1590:1599	the collagen fibril pretension	1570:1599	the collagen fibril pretension	1570:1599	These findings suggest different mechanisms contribute to cartilage viscosity in low and high frequencies, and that the loss of superficial collagen pretension during early OA is due to lower tissue swelling (PG loss), while in advanced OA, both collagen disorganization and lower swelling modulate the collagen fibril pretension.
32648191	3	53	theme	collagen	655:662	arg1	content					664:670	collagen content	655:670	collagen content	655:670	At a low frequency (0.005 Hz), proteoglycan content correlated negatively and collagen content correlated positively with the phase difference (i.e. tissue viscosity).
32648191	2	54	theme	fibril-reinforced	389:405	arg1	properties					430:439	elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties	363:439	elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties	363:439	We established these relationships by comparison of elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties with microscopic and spectroscopic analysis of structure and composition of healthy and osteoarthritic human tibial cartilage (n = 27).
32648191	0	55	theme	Human	63:67	arg1	Cartilage					76:84	Healthy and Osteoarthritic Human Tibial Cartilage	36:84	Healthy and Osteoarthritic Human Tibial Cartilage	36:84	Structure-Function Relationships of Healthy and Osteoarthritic Human Tibial Cartilage: Experimental and Numerical Investigation.
32648191	6	56	theme	initial	1141:1147	arg1	pretension					1180:1189	fibril pretension	1173:1189	fibril pretension	1173:1189	Simultaneously, the initial fibril network modulus (fibril pretension) was smaller in the early and advanced OA groups compared to the healthy group.
32648191	6	56	theme	initial	1141:1147	arg1	smaller					1196:1202	smaller	1196:1202	smaller	1196:1202	Simultaneously, the initial fibril network modulus (fibril pretension) was smaller in the early and advanced OA groups compared to the healthy group.
32648191	6	56	theme	initial	1141:1147	arg1	modulus					1164:1170	the initial fibril network modulus	1137:1170	the initial fibril network modulus (fibril pretension)	1137:1190	Simultaneously, the initial fibril network modulus (fibril pretension) was smaller in the early and advanced OA groups compared to the healthy group.
32648191	7	57	theme	tissue	1463:1468	arg1	swelling					1470:1477	lower tissue swelling	1457:1477	lower tissue swelling (PG loss)	1457:1487	These findings suggest different mechanisms contribute to cartilage viscosity in low and high frequencies, and that the loss of superficial collagen pretension during early OA is due to lower tissue swelling (PG loss), while in advanced OA, both collagen disorganization and lower swelling modulate the collagen fibril pretension.
32648191	7	57	theme	tissue	1463:1468	arg1	loss					1483:1486	PG loss	1480:1486	PG loss	1480:1486	These findings suggest different mechanisms contribute to cartilage viscosity in low and high frequencies, and that the loss of superficial collagen pretension during early OA is due to lower tissue swelling (PG loss), while in advanced OA, both collagen disorganization and lower swelling modulate the collagen fibril pretension.
32648191	7	58	theme	superficial	1399:1409	arg1	pretension					1420:1429	superficial collagen pretension	1399:1429	superficial collagen pretension	1399:1429	These findings suggest different mechanisms contribute to cartilage viscosity in low and high frequencies, and that the loss of superficial collagen pretension during early OA is due to lower tissue swelling (PG loss), while in advanced OA, both collagen disorganization and lower swelling modulate the collagen fibril pretension.
32648191	7	59	theme	high	1360:1363	arg1	frequencies					1365:1375	low and high frequencies	1352:1375	low and high frequencies	1352:1375	These findings suggest different mechanisms contribute to cartilage viscosity in low and high frequencies, and that the loss of superficial collagen pretension during early OA is due to lower tissue swelling (PG loss), while in advanced OA, both collagen disorganization and lower swelling modulate the collagen fibril pretension.
32648191	6	60	theme	healthy	1256:1262	arg1	group					1264:1268	the healthy group	1252:1268	the healthy group	1252:1268	Simultaneously, the initial fibril network modulus (fibril pretension) was smaller in the early and advanced OA groups compared to the healthy group.
32648191	5	61	theme	advanced	1072:1079	arg1	group					1084:1088	the advanced OA group	1068:1088	the advanced OA group	1068:1088	Proteoglycans were lost in the early and advanced OA groups compared to the healthy group, while the superficial collagen orientation angle was greater only in the advanced OA group compared to the healthy group.
32648191	3	62	theme	phase	703:707	arg1	difference					709:718	the phase difference	699:718	the phase difference (i.e. tissue viscosity)	699:742	At a low frequency (0.005 Hz), proteoglycan content correlated negatively and collagen content correlated positively with the phase difference (i.e. tissue viscosity).
32648191	2	63	theme	viscoelastic	372:383	arg1	properties					430:439	elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties	363:439	elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties	363:439	We established these relationships by comparison of elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties with microscopic and spectroscopic analysis of structure and composition of healthy and osteoarthritic human tibial cartilage (n = 27).
32648191	7	64	theme	lower	1457:1461	arg1	swelling					1470:1477	lower tissue swelling	1457:1477	lower tissue swelling (PG loss)	1457:1487	These findings suggest different mechanisms contribute to cartilage viscosity in low and high frequencies, and that the loss of superficial collagen pretension during early OA is due to lower tissue swelling (PG loss), while in advanced OA, both collagen disorganization and lower swelling modulate the collagen fibril pretension.
32648191	7	64	theme	lower	1457:1461	arg1	loss					1483:1486	PG loss	1480:1486	PG loss	1480:1486	These findings suggest different mechanisms contribute to cartilage viscosity in low and high frequencies, and that the loss of superficial collagen pretension during early OA is due to lower tissue swelling (PG loss), while in advanced OA, both collagen disorganization and lower swelling modulate the collagen fibril pretension.
32648191	1	65	theme	osteoarthritis	273:286	arg1	stages					263:268	different stages	253:268	different stages of osteoarthritis (OA)	253:291	Relationships between composition, structure and constituent-specific functional properties of human articular cartilage at different stages of osteoarthritis (OA) are poorly known.
32648191	5	66	theme	OA	1081:1082	arg1	group					1084:1088	the advanced OA group	1068:1088	the advanced OA group	1068:1088	Proteoglycans were lost in the early and advanced OA groups compared to the healthy group, while the superficial collagen orientation angle was greater only in the advanced OA group compared to the healthy group.
32648191	1	67	theme	constituent-specific	178:197	arg1	properties					210:219	constituent-specific functional properties	178:219	constituent-specific functional properties	178:219	Relationships between composition, structure and constituent-specific functional properties of human articular cartilage at different stages of osteoarthritis (OA) are poorly known.
32648191	7	68	dep	suggest	1286:1292	arg1	contribute					1315:1324	contribute	1315:1324	contribute to cartilage viscosity in low and high frequencies	1315:1375	These findings suggest different mechanisms contribute to cartilage viscosity in low and high frequencies, and that the loss of superficial collagen pretension during early OA is due to lower tissue swelling (PG loss), while in advanced OA, both collagen disorganization and lower swelling modulate the collagen fibril pretension.
32648191	7	69	theme	different	1294:1302	arg1	mechanisms					1304:1313	different mechanisms	1294:1313	different mechanisms	1294:1313	These findings suggest different mechanisms contribute to cartilage viscosity in low and high frequencies, and that the loss of superficial collagen pretension during early OA is due to lower tissue swelling (PG loss), while in advanced OA, both collagen disorganization and lower swelling modulate the collagen fibril pretension.
32648191	7	70	theme	lower	1546:1550	arg1	swelling					1552:1559	lower swelling	1546:1559	lower swelling	1546:1559	These findings suggest different mechanisms contribute to cartilage viscosity in low and high frequencies, and that the loss of superficial collagen pretension during early OA is due to lower tissue swelling (PG loss), while in advanced OA, both collagen disorganization and lower swelling modulate the collagen fibril pretension.
32648191	7	71	theme	collagen	1411:1418	arg1	pretension					1420:1429	superficial collagen pretension	1399:1429	superficial collagen pretension	1399:1429	These findings suggest different mechanisms contribute to cartilage viscosity in low and high frequencies, and that the loss of superficial collagen pretension during early OA is due to lower tissue swelling (PG loss), while in advanced OA, both collagen disorganization and lower swelling modulate the collagen fibril pretension.
32648191	7	72	theme	cartilage	1329:1337	arg1	viscosity					1339:1347	cartilage viscosity	1329:1347	cartilage viscosity	1329:1347	These findings suggest different mechanisms contribute to cartilage viscosity in low and high frequencies, and that the loss of superficial collagen pretension during early OA is due to lower tissue swelling (PG loss), while in advanced OA, both collagen disorganization and lower swelling modulate the collagen fibril pretension.
32648191	2	73	theme	spectroscopic	462:474	arg1	analysis					476:483	microscopic and spectroscopic analysis	446:483	analysis	476:483	We established these relationships by comparison of elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties with microscopic and spectroscopic analysis of structure and composition of healthy and osteoarthritic human tibial cartilage (n = 27).
32648191	0	74	theme	Cartilage	76:84	arg1	Relationships					19:31	Structure-Function Relationships	0:31	Structure-Function Relationships of Healthy and Osteoarthritic Human Tibial Cartilage	0:84	Structure-Function Relationships of Healthy and Osteoarthritic Human Tibial Cartilage: Experimental and Numerical Investigation.
32648191	7	75	theme	collagen	1517:1524	arg1	disorganization					1526:1540	collagen disorganization	1517:1540	collagen disorganization	1517:1540	These findings suggest different mechanisms contribute to cartilage viscosity in low and high frequencies, and that the loss of superficial collagen pretension during early OA is due to lower tissue swelling (PG loss), while in advanced OA, both collagen disorganization and lower swelling modulate the collagen fibril pretension.
32648191	4	76	theme	phase	890:894	arg1	difference					896:905	the phase difference	886:905	the phase difference	886:905	At a high-frequency regime (> 0.05 Hz), proteoglycan content correlated negatively and collagen orientation angle correlated positively with the phase difference.
32648191	2	77	theme	properties	430:439	arg1	comparison					349:358	comparison	349:358	comparison of elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties with microscopic and spectroscopic analysis of structure and composition of healthy and osteoarthritic human tibial cartilage (n = 27)	349:574	We established these relationships by comparison of elastic, viscoelastic and fibril-reinforced poroelastic mechanical properties with microscopic and spectroscopic analysis of structure and composition of healthy and osteoarthritic human tibial cartilage (n = 27).
32648191	3	78	dep	viscosity	733:741	arg1	i.e.					721:724	i.e.	721:724	i.e.	721:724	At a low frequency (0.005 Hz), proteoglycan content correlated negatively and collagen content correlated positively with the phase difference (i.e. tissue viscosity).
33541642	4	0	theme	o/w	710:712	arg1	separation					714:723	SiO2-MPs dependent o/w separation	691:723	SiO2-MPs dependent o/w separation	691:723	The composite membrane exhibited SiO2-MPs dependent o/w separation with a high separation efficiency of >99.9 % and a high flux rate of ∼10,660 Lm-2 h-1 while applying a small negative pressure (0.3-0.5 bar).
33541642	5	1	with	membrane	871:878	arg1	content					895:901	different content	885:901	different content of SiO2-MPs	885:913	The membrane with different content of SiO2-MPs also showed the potential to separate oil-in-water emulsion with the highest oil rejection of 98.2 % and the highest flux rate of ∼1250 Lm-2 h-1 on an ultra-low pressure <0.1 bar.
33541642	2	2	theme	cellulose	371:379	arg1	membrane					442:449	bacterial cellulose (BC) based superhydrophilic/underwater superoleophobic (SUS) membrane	361:449	bacterial cellulose (BC) based superhydrophilic/underwater superoleophobic (SUS) membrane	361:449	In this study, we have developed bacterial cellulose (BC) based superhydrophilic/underwater superoleophobic (SUS) membrane for o/w separation.
33541642	2	3	theme	bacterial	361:369	arg1	membrane					442:449	bacterial cellulose (BC) based superhydrophilic/underwater superoleophobic (SUS) membrane	361:449	bacterial cellulose (BC) based superhydrophilic/underwater superoleophobic (SUS) membrane	361:449	In this study, we have developed bacterial cellulose (BC) based superhydrophilic/underwater superoleophobic (SUS) membrane for o/w separation.
33541642	3	4	theme	BC	533:534	arg1	nanofibers					536:545	BC nanofibers	533:545	BC nanofibers	533:545	The membrane was designed through a facile method by blending BC nanofibers with silica microparticles (SiO2-MPs), which was further modified by bio-inspired polydopamine (PDA) coatings.
33541642	4	5	theme	high	776:779	arg1	rate					786:789	a high flux rate	774:789	a high flux rate of ∼10,660 Lm-2 h-1	774:809	The composite membrane exhibited SiO2-MPs dependent o/w separation with a high separation efficiency of >99.9 % and a high flux rate of ∼10,660 Lm-2 h-1 while applying a small negative pressure (0.3-0.5 bar).
33541642	4	6	theme	%	768:768	arg1	rate					786:789	a high flux rate	774:789	a high flux rate of ∼10,660 Lm-2 h-1	774:809	The composite membrane exhibited SiO2-MPs dependent o/w separation with a high separation efficiency of >99.9 % and a high flux rate of ∼10,660 Lm-2 h-1 while applying a small negative pressure (0.3-0.5 bar).
33541642	4	6	theme	%	768:768	arg1	efficiency					748:757	a high separation efficiency	730:757	a high separation efficiency of >99.9 %	730:768	The composite membrane exhibited SiO2-MPs dependent o/w separation with a high separation efficiency of >99.9 % and a high flux rate of ∼10,660 Lm-2 h-1 while applying a small negative pressure (0.3-0.5 bar).
33541642	5	7	theme	separate	944:951	arg1	emulsion					966:973	separate oil-in-water emulsion	944:973	separate oil-in-water emulsion with the highest oil rejection of 98.2 %	944:1014	The membrane with different content of SiO2-MPs also showed the potential to separate oil-in-water emulsion with the highest oil rejection of 98.2 % and the highest flux rate of ∼1250 Lm-2 h-1 on an ultra-low pressure <0.1 bar.
33541642	5	8	theme	highest	984:990	arg1	rejection					996:1004	the highest oil rejection	980:1004	the highest oil rejection of 98.2 %	980:1014	The membrane with different content of SiO2-MPs also showed the potential to separate oil-in-water emulsion with the highest oil rejection of 98.2 % and the highest flux rate of ∼1250 Lm-2 h-1 on an ultra-low pressure <0.1 bar.
33541642	5	9	theme	SiO2-MPs	906:913	arg1	content					895:901	different content	885:901	different content of SiO2-MPs	885:913	The membrane with different content of SiO2-MPs also showed the potential to separate oil-in-water emulsion with the highest oil rejection of 98.2 % and the highest flux rate of ∼1250 Lm-2 h-1 on an ultra-low pressure <0.1 bar.
33541642	4	10	theme	separation	737:746	arg1	efficiency					748:757	a high separation efficiency	730:757	a high separation efficiency of >99.9 %	730:768	The composite membrane exhibited SiO2-MPs dependent o/w separation with a high separation efficiency of >99.9 % and a high flux rate of ∼10,660 Lm-2 h-1 while applying a small negative pressure (0.3-0.5 bar).
33541642	4	11	theme	high	732:735	arg1	efficiency					748:757	a high separation efficiency	730:757	a high separation efficiency of >99.9 %	730:768	The composite membrane exhibited SiO2-MPs dependent o/w separation with a high separation efficiency of >99.9 % and a high flux rate of ∼10,660 Lm-2 h-1 while applying a small negative pressure (0.3-0.5 bar).
33541642	5	12	theme	oil	992:994	arg1	rejection					996:1004	the highest oil rejection	980:1004	the highest oil rejection of 98.2 %	980:1014	The membrane with different content of SiO2-MPs also showed the potential to separate oil-in-water emulsion with the highest oil rejection of 98.2 % and the highest flux rate of ∼1250 Lm-2 h-1 on an ultra-low pressure <0.1 bar.
33541642	1	13	theme	increasing	186:195	arg1	contamination					203:215	the increasing water contamination	182:215	the increasing water contamination by oil spill accidents, and oil-containing wastewater produced by food, textile, and petrochemical industries	182:325	The oil/water (o/w) separation is a global challenge because of the increasing water contamination by oil spill accidents, and oil-containing wastewater produced by food, textile, and petrochemical industries.
33541642	3	14	theme	silica	552:557	arg1	microparticles					559:572	silica microparticles	552:572	silica microparticles (SiO2-MPs)	552:583	The membrane was designed through a facile method by blending BC nanofibers with silica microparticles (SiO2-MPs), which was further modified by bio-inspired polydopamine (PDA) coatings.
33541642	3	14	theme	silica	552:557	arg1	SiO2-MPs					575:582	SiO2-MPs	575:582	SiO2-MPs	575:582	The membrane was designed through a facile method by blending BC nanofibers with silica microparticles (SiO2-MPs), which was further modified by bio-inspired polydopamine (PDA) coatings.
33541642	1	15	theme	water	197:201	arg1	contamination					203:215	the increasing water contamination	182:215	the increasing water contamination by oil spill accidents, and oil-containing wastewater produced by food, textile, and petrochemical industries	182:325	The oil/water (o/w) separation is a global challenge because of the increasing water contamination by oil spill accidents, and oil-containing wastewater produced by food, textile, and petrochemical industries.
33541642	5	16	with	emulsion	966:973	arg1	rejection					996:1004	the highest oil rejection	980:1004	the highest oil rejection of 98.2 %	980:1014	The membrane with different content of SiO2-MPs also showed the potential to separate oil-in-water emulsion with the highest oil rejection of 98.2 % and the highest flux rate of ∼1250 Lm-2 h-1 on an ultra-low pressure <0.1 bar.
33541642	4	17	theme	composite	662:670	arg1	membrane					672:679	The composite membrane	658:679	The composite membrane	658:679	The composite membrane exhibited SiO2-MPs dependent o/w separation with a high separation efficiency of >99.9 % and a high flux rate of ∼10,660 Lm-2 h-1 while applying a small negative pressure (0.3-0.5 bar).
33541642	1	18	theme	oil/water	122:130	arg1	separation					138:147	The oil/water (o/w) separation	118:147	The oil/water (o/w) separation	118:147	The oil/water (o/w) separation is a global challenge because of the increasing water contamination by oil spill accidents, and oil-containing wastewater produced by food, textile, and petrochemical industries.
33541642	1	18	theme	oil/water	122:130	arg1	challenge					161:169	a global challenge	152:169	a global challenge	152:169	The oil/water (o/w) separation is a global challenge because of the increasing water contamination by oil spill accidents, and oil-containing wastewater produced by food, textile, and petrochemical industries.
33541642	2	19	theme	based	386:390	arg1	membrane					442:449	bacterial cellulose (BC) based superhydrophilic/underwater superoleophobic (SUS) membrane	361:449	bacterial cellulose (BC) based superhydrophilic/underwater superoleophobic (SUS) membrane	361:449	In this study, we have developed bacterial cellulose (BC) based superhydrophilic/underwater superoleophobic (SUS) membrane for o/w separation.
33541642	5	20	theme	oil-in-water	953:964	arg1	emulsion					966:973	separate oil-in-water emulsion	944:973	separate oil-in-water emulsion with the highest oil rejection of 98.2 %	944:1014	The membrane with different content of SiO2-MPs also showed the potential to separate oil-in-water emulsion with the highest oil rejection of 98.2 % and the highest flux rate of ∼1250 Lm-2 h-1 on an ultra-low pressure <0.1 bar.
33541642	5	21	theme	different	885:893	arg1	content					895:901	different content	885:901	different content of SiO2-MPs	885:913	The membrane with different content of SiO2-MPs also showed the potential to separate oil-in-water emulsion with the highest oil rejection of 98.2 % and the highest flux rate of ∼1250 Lm-2 h-1 on an ultra-low pressure <0.1 bar.
33541642	0	22	theme	cellulose-based	23:37	arg1	superhydrophilic/underwater					39:65	bacterial cellulose-based superhydrophilic/underwater	13:65	bacterial cellulose-based superhydrophilic/underwater	13:65	Designing of bacterial cellulose-based superhydrophilic/underwater superoleophobic membrane for oil/water separation.
33541642	5	23	theme	%	1014:1014	arg1	rejection					996:1004	the highest oil rejection	980:1004	the highest oil rejection of 98.2 %	980:1014	The membrane with different content of SiO2-MPs also showed the potential to separate oil-in-water emulsion with the highest oil rejection of 98.2 % and the highest flux rate of ∼1250 Lm-2 h-1 on an ultra-low pressure <0.1 bar.
33541642	1	24	theme	o/w	133:135	arg1	separation					138:147	The oil/water (o/w) separation	118:147	The oil/water (o/w) separation	118:147	The oil/water (o/w) separation is a global challenge because of the increasing water contamination by oil spill accidents, and oil-containing wastewater produced by food, textile, and petrochemical industries.
33541642	1	24	theme	o/w	133:135	arg1	challenge					161:169	a global challenge	152:169	a global challenge	152:169	The oil/water (o/w) separation is a global challenge because of the increasing water contamination by oil spill accidents, and oil-containing wastewater produced by food, textile, and petrochemical industries.
33541642	1	25	theme	oil	220:222	arg1	accidents					230:238	oil spill accidents	220:238	oil spill accidents	220:238	The oil/water (o/w) separation is a global challenge because of the increasing water contamination by oil spill accidents, and oil-containing wastewater produced by food, textile, and petrochemical industries.
33541642	0	26	theme	bacterial	13:21	arg1	superhydrophilic/underwater					39:65	bacterial cellulose-based superhydrophilic/underwater	13:65	bacterial cellulose-based superhydrophilic/underwater	13:65	Designing of bacterial cellulose-based superhydrophilic/underwater superoleophobic membrane for oil/water separation.
33541642	3	27	mod	modified	604:611	arg1	microparticles					559:572	silica microparticles	552:572	silica microparticles (SiO2-MPs)	552:583	The membrane was designed through a facile method by blending BC nanofibers with silica microparticles (SiO2-MPs), which was further modified by bio-inspired polydopamine (PDA) coatings.
33541642	3	27	mod	modified	604:611	arg3	coatings					648:655	bio-inspired polydopamine (PDA) coatings	616:655	bio-inspired polydopamine (PDA) coatings	616:655	The membrane was designed through a facile method by blending BC nanofibers with silica microparticles (SiO2-MPs), which was further modified by bio-inspired polydopamine (PDA) coatings.
33541642	3	27	mod	modified	604:611	arg1	SiO2-MPs					575:582	SiO2-MPs	575:582	SiO2-MPs	575:582	The membrane was designed through a facile method by blending BC nanofibers with silica microparticles (SiO2-MPs), which was further modified by bio-inspired polydopamine (PDA) coatings.
33541642	1	28	theme	spill	224:228	arg1	accidents					230:238	oil spill accidents	220:238	oil spill accidents	220:238	The oil/water (o/w) separation is a global challenge because of the increasing water contamination by oil spill accidents, and oil-containing wastewater produced by food, textile, and petrochemical industries.
33541642	5	29	theme	ultra-low	1066:1074	arg1	<0.1 bar					1085:1092	an ultra-low pressure <0.1 bar	1063:1092	an ultra-low pressure <0.1 bar	1063:1092	The membrane with different content of SiO2-MPs also showed the potential to separate oil-in-water emulsion with the highest oil rejection of 98.2 % and the highest flux rate of ∼1250 Lm-2 h-1 on an ultra-low pressure <0.1 bar.
33541642	4	30	theme	small	828:832	arg1	0.3-0.5 bar					853:863	0.3-0.5 bar	853:863	0.3-0.5 bar	853:863	The composite membrane exhibited SiO2-MPs dependent o/w separation with a high separation efficiency of >99.9 % and a high flux rate of ∼10,660 Lm-2 h-1 while applying a small negative pressure (0.3-0.5 bar).
33541642	4	30	theme	small	828:832	arg1	pressure					843:850	a small negative pressure	826:850	a small negative pressure (0.3-0.5 bar)	826:864	The composite membrane exhibited SiO2-MPs dependent o/w separation with a high separation efficiency of >99.9 % and a high flux rate of ∼10,660 Lm-2 h-1 while applying a small negative pressure (0.3-0.5 bar).
33541642	2	31	theme	SUS	437:439	arg1	membrane					442:449	bacterial cellulose (BC) based superhydrophilic/underwater superoleophobic (SUS) membrane	361:449	bacterial cellulose (BC) based superhydrophilic/underwater superoleophobic (SUS) membrane	361:449	In this study, we have developed bacterial cellulose (BC) based superhydrophilic/underwater superoleophobic (SUS) membrane for o/w separation.
33541642	2	32	theme	superoleophobic	420:434	arg1	membrane					442:449	bacterial cellulose (BC) based superhydrophilic/underwater superoleophobic (SUS) membrane	361:449	bacterial cellulose (BC) based superhydrophilic/underwater superoleophobic (SUS) membrane	361:449	In this study, we have developed bacterial cellulose (BC) based superhydrophilic/underwater superoleophobic (SUS) membrane for o/w separation.
33541642	0	33	theme	superhydrophilic/underwater	39:65	arg1	Designing					0:8	Designing	0:8	Designing	0:8	Designing of bacterial cellulose-based superhydrophilic/underwater superoleophobic membrane for oil/water separation.
33541642	5	34	theme	pressure	1076:1083	arg1	<0.1 bar					1085:1092	an ultra-low pressure <0.1 bar	1063:1092	an ultra-low pressure <0.1 bar	1063:1092	The membrane with different content of SiO2-MPs also showed the potential to separate oil-in-water emulsion with the highest oil rejection of 98.2 % and the highest flux rate of ∼1250 Lm-2 h-1 on an ultra-low pressure <0.1 bar.
33541642	2	35	theme	o/w	455:457	arg1	separation					459:468	o/w separation	455:468	o/w separation	455:468	In this study, we have developed bacterial cellulose (BC) based superhydrophilic/underwater superoleophobic (SUS) membrane for o/w separation.
33541642	5	36	theme	flux	1032:1035	arg1	rate					1037:1040	the highest flux rate	1020:1040	the highest flux rate of ∼1250 Lm-2 h-1	1020:1058	The membrane with different content of SiO2-MPs also showed the potential to separate oil-in-water emulsion with the highest oil rejection of 98.2 % and the highest flux rate of ∼1250 Lm-2 h-1 on an ultra-low pressure <0.1 bar.
33541642	3	37	theme	facile	507:512	arg1	method					514:519	a facile method	505:519	a facile method	505:519	The membrane was designed through a facile method by blending BC nanofibers with silica microparticles (SiO2-MPs), which was further modified by bio-inspired polydopamine (PDA) coatings.
33541642	6	38	theme	harsh	1181:1185	arg1	conditions					1187:1196	harsh conditions	1181:1196	harsh conditions	1181:1196	Moreover, the membrane showed antifouling properties, recyclability, and stability in harsh conditions.
33541642	4	39	theme	negative	834:841	arg1	0.3-0.5 bar					853:863	0.3-0.5 bar	853:863	0.3-0.5 bar	853:863	The composite membrane exhibited SiO2-MPs dependent o/w separation with a high separation efficiency of >99.9 % and a high flux rate of ∼10,660 Lm-2 h-1 while applying a small negative pressure (0.3-0.5 bar).
33541642	4	39	theme	negative	834:841	arg1	pressure					843:850	a small negative pressure	826:850	a small negative pressure (0.3-0.5 bar)	826:864	The composite membrane exhibited SiO2-MPs dependent o/w separation with a high separation efficiency of >99.9 % and a high flux rate of ∼10,660 Lm-2 h-1 while applying a small negative pressure (0.3-0.5 bar).
33541642	6	40	theme	antifouling	1125:1135	arg1	properties					1137:1146	antifouling properties	1125:1146	antifouling properties	1125:1146	Moreover, the membrane showed antifouling properties, recyclability, and stability in harsh conditions.
33541642	4	41	theme	∼10,660 Lm-2 h-1	794:809	arg1	rate					786:789	a high flux rate	774:789	a high flux rate of ∼10,660 Lm-2 h-1	774:809	The composite membrane exhibited SiO2-MPs dependent o/w separation with a high separation efficiency of >99.9 % and a high flux rate of ∼10,660 Lm-2 h-1 while applying a small negative pressure (0.3-0.5 bar).
33541642	4	41	theme	∼10,660 Lm-2 h-1	794:809	arg1	efficiency					748:757	a high separation efficiency	730:757	a high separation efficiency of >99.9 %	730:768	The composite membrane exhibited SiO2-MPs dependent o/w separation with a high separation efficiency of >99.9 % and a high flux rate of ∼10,660 Lm-2 h-1 while applying a small negative pressure (0.3-0.5 bar).
33541642	5	42	theme	highest	1024:1030	arg1	rate					1037:1040	the highest flux rate	1020:1040	the highest flux rate of ∼1250 Lm-2 h-1	1020:1058	The membrane with different content of SiO2-MPs also showed the potential to separate oil-in-water emulsion with the highest oil rejection of 98.2 % and the highest flux rate of ∼1250 Lm-2 h-1 on an ultra-low pressure <0.1 bar.
33541642	4	43	theme	flux	781:784	arg1	rate					786:789	a high flux rate	774:789	a high flux rate of ∼10,660 Lm-2 h-1	774:809	The composite membrane exhibited SiO2-MPs dependent o/w separation with a high separation efficiency of >99.9 % and a high flux rate of ∼10,660 Lm-2 h-1 while applying a small negative pressure (0.3-0.5 bar).
33541642	5	44	theme	Lm-2 h-1	1051:1058	arg1	potential					931:939	the potential	927:939	the potential to separate oil-in-water emulsion with the highest oil rejection of 98.2 %	927:1014	The membrane with different content of SiO2-MPs also showed the potential to separate oil-in-water emulsion with the highest oil rejection of 98.2 % and the highest flux rate of ∼1250 Lm-2 h-1 on an ultra-low pressure <0.1 bar.
33541642	5	44	theme	Lm-2 h-1	1051:1058	arg1	rate					1037:1040	the highest flux rate	1020:1040	the highest flux rate of ∼1250 Lm-2 h-1	1020:1058	The membrane with different content of SiO2-MPs also showed the potential to separate oil-in-water emulsion with the highest oil rejection of 98.2 % and the highest flux rate of ∼1250 Lm-2 h-1 on an ultra-low pressure <0.1 bar.
33541642	3	45	theme	PDA	643:645	arg1	coatings					648:655	bio-inspired polydopamine (PDA) coatings	616:655	bio-inspired polydopamine (PDA) coatings	616:655	The membrane was designed through a facile method by blending BC nanofibers with silica microparticles (SiO2-MPs), which was further modified by bio-inspired polydopamine (PDA) coatings.
33541642	1	46	theme	global	154:159	arg1	separation					138:147	The oil/water (o/w) separation	118:147	The oil/water (o/w) separation	118:147	The oil/water (o/w) separation is a global challenge because of the increasing water contamination by oil spill accidents, and oil-containing wastewater produced by food, textile, and petrochemical industries.
33541642	1	46	theme	global	154:159	arg1	challenge					161:169	a global challenge	152:169	a global challenge	152:169	The oil/water (o/w) separation is a global challenge because of the increasing water contamination by oil spill accidents, and oil-containing wastewater produced by food, textile, and petrochemical industries.
33541642	1	47	theme	oil-containing	245:258	arg1	wastewater					260:269	oil-containing wastewater	245:269	oil-containing wastewater	245:269	The oil/water (o/w) separation is a global challenge because of the increasing water contamination by oil spill accidents, and oil-containing wastewater produced by food, textile, and petrochemical industries.
33541642	1	48	dep	food	283:286	arg1	industries					316:325	industries	316:325	industries	316:325	The oil/water (o/w) separation is a global challenge because of the increasing water contamination by oil spill accidents, and oil-containing wastewater produced by food, textile, and petrochemical industries.
33541642	3	49	theme	bio-inspired	616:627	arg1	coatings					648:655	bio-inspired polydopamine (PDA) coatings	616:655	bio-inspired polydopamine (PDA) coatings	616:655	The membrane was designed through a facile method by blending BC nanofibers with silica microparticles (SiO2-MPs), which was further modified by bio-inspired polydopamine (PDA) coatings.
33541642	2	50	theme	BC	382:383	arg1	membrane					442:449	bacterial cellulose (BC) based superhydrophilic/underwater superoleophobic (SUS) membrane	361:449	bacterial cellulose (BC) based superhydrophilic/underwater superoleophobic (SUS) membrane	361:449	In this study, we have developed bacterial cellulose (BC) based superhydrophilic/underwater superoleophobic (SUS) membrane for o/w separation.
33541642	2	51	theme	superhydrophilic/underwater	392:418	arg1	membrane					442:449	bacterial cellulose (BC) based superhydrophilic/underwater superoleophobic (SUS) membrane	361:449	bacterial cellulose (BC) based superhydrophilic/underwater superoleophobic (SUS) membrane	361:449	In this study, we have developed bacterial cellulose (BC) based superhydrophilic/underwater superoleophobic (SUS) membrane for o/w separation.
33541642	0	52	theme	oil/water	96:104	arg1	separation					106:115	oil/water separation	96:115	oil/water separation	96:115	Designing of bacterial cellulose-based superhydrophilic/underwater superoleophobic membrane for oil/water separation.
33541642	3	53	theme	polydopamine	629:640	arg1	coatings					648:655	bio-inspired polydopamine (PDA) coatings	616:655	bio-inspired polydopamine (PDA) coatings	616:655	The membrane was designed through a facile method by blending BC nanofibers with silica microparticles (SiO2-MPs), which was further modified by bio-inspired polydopamine (PDA) coatings.
33541642	4	54	theme	dependent	700:708	arg1	separation					714:723	SiO2-MPs dependent o/w separation	691:723	SiO2-MPs dependent o/w separation	691:723	The composite membrane exhibited SiO2-MPs dependent o/w separation with a high separation efficiency of >99.9 % and a high flux rate of ∼10,660 Lm-2 h-1 while applying a small negative pressure (0.3-0.5 bar).
32737882	6	0	theme	production	1025:1034	arg1	halftime					998:1005	The halftime	994:1005	The halftime of asymptotic gas production	994:1034	The halftime of asymptotic gas production ranked as: KF < MCS = LC (P < 0.001).
32737882	11	1	from	times	2016:2020	arg1	related					1932:1938	related	1932:1938	related	1932:1938	In addition, we also observed that the abundances of certain specific bacteria (Anaerovibrio and Erysipelatoclostridium) were closely related to the SCFAs production (propionate and butyrate) at different fermentation times.
32737882	11	1	from	times	2016:2020	arg1	propionate					1965:1974	propionate	1965:1974	propionate	1965:1974	In addition, we also observed that the abundances of certain specific bacteria (Anaerovibrio and Erysipelatoclostridium) were closely related to the SCFAs production (propionate and butyrate) at different fermentation times.
32737882	11	1	from	times	2016:2020	arg1	butyrate					1980:1987	butyrate	1980:1987	butyrate	1980:1987	In addition, we also observed that the abundances of certain specific bacteria (Anaerovibrio and Erysipelatoclostridium) were closely related to the SCFAs production (propionate and butyrate) at different fermentation times.
32737882	11	1	from	times	2016:2020	arg1	abundances					1837:1846	the abundances	1833:1846	the abundances of certain specific bacteria (Anaerovibrio and Erysipelatoclostridium)	1833:1917	In addition, we also observed that the abundances of certain specific bacteria (Anaerovibrio and Erysipelatoclostridium) were closely related to the SCFAs production (propionate and butyrate) at different fermentation times.
32737882	1	2	theme	resource	257:264	arg1	utilization					266:276	feed resource utilization	252:276	feed resource utilization	252:276	BACKGROUND Efficient utilization of dietary fibers (DFs) is important for optimizing feed resource utilization and animal health.
32737882	7	3	theme	acid	1148:1151	arg1	concentrations					1123:1136	higher concentrations	1116:1136	higher concentrations of formic acid and lactate	1116:1163	At 36 h of fermentation, MCS group showed higher concentrations of formic acid and lactate than LC and KF groups, whereas KF group showed higher concentrations of propionate and butyrate than LC and MCS groups (P < 0.05).
32737882	4	4	theme	fatty	798:802	arg1	acids					804:808	short-chain fatty acids	786:808	short-chain fatty acids (SCFAs)	786:816	Gas production, short-chain fatty acids (SCFAs) profiles and microbial composition were monitored during the fermentation.
32737882	4	4	theme	fatty	798:802	arg1	production					774:783	Gas production	770:783	Gas production	770:783	Gas production, short-chain fatty acids (SCFAs) profiles and microbial composition were monitored during the fermentation.
32737882	4	4	theme	fatty	798:802	arg1	SCFAs					811:815	SCFAs	811:815	SCFAs	811:815	Gas production, short-chain fatty acids (SCFAs) profiles and microbial composition were monitored during the fermentation.
32737882	13	5	theme	SCFA	2348:2351	arg1	production					2353:2362	SCFA production	2348:2362	SCFA production	2348:2362	In addition, the fermentation of DFs with different physicochemical properties had divergent impacts on microbial composition and SCFA production.
32737882	15	6	theme	Chemical	2576:2583	arg1	Industry					2585:2592	Chemical Industry	2576:2592	Chemical Industry	2576:2592	© 2020 Society of Chemical Industry.
32737882	4	7	theme	microbial	831:839	arg1	composition					841:851	microbial composition	831:851	microbial composition	831:851	Gas production, short-chain fatty acids (SCFAs) profiles and microbial composition were monitored during the fermentation.
32737882	11	8	theme	specific	1859:1866	arg1	Anaerovibrio					1878:1889	Anaerovibrio	1878:1889	Anaerovibrio	1878:1889	In addition, we also observed that the abundances of certain specific bacteria (Anaerovibrio and Erysipelatoclostridium) were closely related to the SCFAs production (propionate and butyrate) at different fermentation times.
32737882	11	8	theme	specific	1859:1866	arg1	bacteria					1868:1875	certain specific bacteria	1851:1875	certain specific bacteria (Anaerovibrio and Erysipelatoclostridium)	1851:1917	In addition, we also observed that the abundances of certain specific bacteria (Anaerovibrio and Erysipelatoclostridium) were closely related to the SCFAs production (propionate and butyrate) at different fermentation times.
32737882	11	8	theme	specific	1859:1866	arg1	Erysipelatoclostridium					1895:1916	Erysipelatoclostridium	1895:1916	Erysipelatoclostridium	1895:1916	In addition, we also observed that the abundances of certain specific bacteria (Anaerovibrio and Erysipelatoclostridium) were closely related to the SCFAs production (propionate and butyrate) at different fermentation times.
32737882	1	9	theme	dietary	203:209	arg1	DFs					219:221	DFs	219:221	DFs	219:221	BACKGROUND Efficient utilization of dietary fibers (DFs) is important for optimizing feed resource utilization and animal health.
32737882	1	9	theme	dietary	203:209	arg1	fibers					211:216	dietary fibers	203:216	dietary fibers (DFs)	203:222	BACKGROUND Efficient utilization of dietary fibers (DFs) is important for optimizing feed resource utilization and animal health.
32737882	14	10	theme	new	2489:2491	arg1	ideas					2493:2497	new ideas	2489:2497	new ideas for the rational use of fiber resources in lactating sows	2489:2555	These findings deepen our understanding of the mechanisms of interaction between DFs and intestinal microbiota, and provide new ideas for the rational use of fiber resources in lactating sows.
32737882	10	11	theme	KF	1787:1788	arg1	groups					1790:1795	KF groups	1787:1795	KF groups	1787:1795	At 72 h of fermentation, the abundance of Fibrobacter in LC group was higher than that in MCS and KF groups.
32737882	11	12	theme	bacteria	1868:1875	arg1	related					1932:1938	related	1932:1938	related	1932:1938	In addition, we also observed that the abundances of certain specific bacteria (Anaerovibrio and Erysipelatoclostridium) were closely related to the SCFAs production (propionate and butyrate) at different fermentation times.
32737882	11	12	theme	bacteria	1868:1875	arg1	propionate					1965:1974	propionate	1965:1974	propionate	1965:1974	In addition, we also observed that the abundances of certain specific bacteria (Anaerovibrio and Erysipelatoclostridium) were closely related to the SCFAs production (propionate and butyrate) at different fermentation times.
32737882	11	12	theme	bacteria	1868:1875	arg1	butyrate					1980:1987	butyrate	1980:1987	butyrate	1980:1987	In addition, we also observed that the abundances of certain specific bacteria (Anaerovibrio and Erysipelatoclostridium) were closely related to the SCFAs production (propionate and butyrate) at different fermentation times.
32737882	11	12	theme	bacteria	1868:1875	arg1	abundances					1837:1846	the abundances	1833:1846	the abundances of certain specific bacteria (Anaerovibrio and Erysipelatoclostridium)	1833:1917	In addition, we also observed that the abundances of certain specific bacteria (Anaerovibrio and Erysipelatoclostridium) were closely related to the SCFAs production (propionate and butyrate) at different fermentation times.
32737882	7	13	theme	MCS	1273:1275	arg1	P < 0.05					1285:1292	P < 0.05	1285:1292	P < 0.05	1285:1292	At 36 h of fermentation, MCS group showed higher concentrations of formic acid and lactate than LC and KF groups, whereas KF group showed higher concentrations of propionate and butyrate than LC and MCS groups (P < 0.05).
32737882	7	13	theme	MCS	1273:1275	arg1	groups					1277:1282	MCS groups	1273:1282	MCS groups (P < 0.05)	1273:1293	At 36 h of fermentation, MCS group showed higher concentrations of formic acid and lactate than LC and KF groups, whereas KF group showed higher concentrations of propionate and butyrate than LC and MCS groups (P < 0.05).
32737882	3	14	theme	fermentable	720:730	arg1	DFs					612:614	three different DFs	596:614	three different DFs	596:614	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	14	theme	fermentable	720:730	arg1	lignocellulose					617:630	lignocellulose	617:630	lignocellulose (LC)	617:635	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	14	theme	fermentable	720:730	arg1	starch					655:660	modified cassava starch	638:660	modified cassava starch (MCS)	638:666	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	14	theme	fermentable	720:730	arg1	flour					679:683	konjac flour	672:683	konjac flour (KF)	672:688	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	14	theme	fermentable	720:730	arg1	fiber					732:736	fermentable fiber	720:736	fermentable fiber	720:736	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	0	15	theme	lactating	142:150	arg1	sows					152:155	lactating sows	142:155	lactating sows in vitro	142:164	Effects of dietary fibers with different physicochemical properties on fermentation kinetics and microbial composition by fecal inoculum from lactating sows in vitro.
32737882	8	16	theme	higher	1337:1342	arg1	concentrations					1344:1357	higher concentrations	1337:1357	higher concentrations of formic acid, lactate and propionate	1337:1396	At 72 h of fermentation, KF group showed higher concentrations of formic acid, lactate and propionate than LC and MCS groups, whereas MCS group showed higher concentrations of acetate and butyrate than LC and KF groups (P < 0.05).
32737882	7	17	theme	butyrate	1252:1259	arg1	concentrations					1219:1232	higher concentrations	1212:1232	higher concentrations of propionate and butyrate	1212:1259	At 36 h of fermentation, MCS group showed higher concentrations of formic acid and lactate than LC and KF groups, whereas KF group showed higher concentrations of propionate and butyrate than LC and MCS groups (P < 0.05).
32737882	13	18	theme	divergent	2301:2309	arg1	impacts					2311:2317	divergent impacts	2301:2317	divergent impacts	2301:2317	In addition, the fermentation of DFs with different physicochemical properties had divergent impacts on microbial composition and SCFA production.
32737882	2	19	theme	in	491:492	arg1	fermentation					500:511	in vitro fermentation	491:511	in vitro fermentation by fecal inoculum from lactating sow	491:548	The aim of the current study was to assess the effects of DFs with varying physicochemical properties (bulky, viscous, and fermentable) on fermentation kinetics and microbial composition during in vitro fermentation by fecal inoculum from lactating sow.
32737882	7	20	theme	propionate	1237:1246	arg1	concentrations					1219:1232	higher concentrations	1212:1232	higher concentrations of propionate and butyrate	1212:1259	At 36 h of fermentation, MCS group showed higher concentrations of formic acid and lactate than LC and KF groups, whereas KF group showed higher concentrations of propionate and butyrate than LC and MCS groups (P < 0.05).
32737882	7	21	theme	formic	1141:1146	arg1	acid					1148:1151	formic acid	1141:1151	formic acid	1141:1151	At 36 h of fermentation, MCS group showed higher concentrations of formic acid and lactate than LC and KF groups, whereas KF group showed higher concentrations of propionate and butyrate than LC and MCS groups (P < 0.05).
32737882	9	22	theme	fermentation	1538:1549	arg1	36 h					1530:1533	36 h	1530:1533	36 h of fermentation	1530:1549	At 36 h of fermentation, Anaerovibrio and Erysipelatoclostridium abundances were higher in KF group, whereas Proteiniclasticum abundance was higher in MCS group.
32737882	9	23	theme	KF	1618:1619	arg1	group					1621:1625	KF group	1618:1625	KF group	1618:1625	At 36 h of fermentation, Anaerovibrio and Erysipelatoclostridium abundances were higher in KF group, whereas Proteiniclasticum abundance was higher in MCS group.
32737882	8	24	theme	acid	1369:1372	arg1	concentrations					1344:1357	higher concentrations	1337:1357	higher concentrations of formic acid, lactate and propionate	1337:1396	At 72 h of fermentation, KF group showed higher concentrations of formic acid, lactate and propionate than LC and MCS groups, whereas MCS group showed higher concentrations of acetate and butyrate than LC and KF groups (P < 0.05).
32737882	11	25	theme	certain	1851:1857	arg1	Anaerovibrio					1878:1889	Anaerovibrio	1878:1889	Anaerovibrio	1878:1889	In addition, we also observed that the abundances of certain specific bacteria (Anaerovibrio and Erysipelatoclostridium) were closely related to the SCFAs production (propionate and butyrate) at different fermentation times.
32737882	11	25	theme	certain	1851:1857	arg1	bacteria					1868:1875	certain specific bacteria	1851:1875	certain specific bacteria (Anaerovibrio and Erysipelatoclostridium)	1851:1917	In addition, we also observed that the abundances of certain specific bacteria (Anaerovibrio and Erysipelatoclostridium) were closely related to the SCFAs production (propionate and butyrate) at different fermentation times.
32737882	11	25	theme	certain	1851:1857	arg1	Erysipelatoclostridium					1895:1916	Erysipelatoclostridium	1895:1916	Erysipelatoclostridium	1895:1916	In addition, we also observed that the abundances of certain specific bacteria (Anaerovibrio and Erysipelatoclostridium) were closely related to the SCFAs production (propionate and butyrate) at different fermentation times.
32737882	5	26	dep	RESULTS	893:899	arg1	Results					901:907	Results	901:907	RESULTS Results	893:907	RESULTS Results showed that the gas production in 72 h (GP72h ) ranked as: KF > MCS > LC (P < 0.05).
32737882	2	27	theme	fecal	516:520	arg1	inoculum					522:529	fecal inoculum	516:529	fecal inoculum from lactating sow	516:548	The aim of the current study was to assess the effects of DFs with varying physicochemical properties (bulky, viscous, and fermentable) on fermentation kinetics and microbial composition during in vitro fermentation by fecal inoculum from lactating sow.
32737882	8	28	theme	lactate	1375:1381	arg1	concentrations					1344:1357	higher concentrations	1337:1357	higher concentrations of formic acid, lactate and propionate	1337:1396	At 72 h of fermentation, KF group showed higher concentrations of formic acid, lactate and propionate than LC and MCS groups, whereas MCS group showed higher concentrations of acetate and butyrate than LC and KF groups (P < 0.05).
32737882	0	29	theme	microbial	97:105	arg1	composition					107:117	microbial composition	97:117	microbial composition by fecal inoculum from lactating sows in vitro	97:164	Effects of dietary fibers with different physicochemical properties on fermentation kinetics and microbial composition by fecal inoculum from lactating sows in vitro.
32737882	5	30	theme	gas	925:927	arg1	production					929:938	the gas production	921:938	the gas production in 72 h (GP72h )	921:955	RESULTS Results showed that the gas production in 72 h (GP72h ) ranked as: KF > MCS > LC (P < 0.05).
32737882	9	31	theme	Proteiniclasticum	1636:1652	arg1	abundance					1654:1662	Proteiniclasticum abundance	1636:1662	Proteiniclasticum abundance	1636:1662	At 36 h of fermentation, Anaerovibrio and Erysipelatoclostridium abundances were higher in KF group, whereas Proteiniclasticum abundance was higher in MCS group.
32737882	8	32	theme	propionate	1387:1396	arg1	concentrations					1344:1357	higher concentrations	1337:1357	higher concentrations of formic acid, lactate and propionate	1337:1396	At 72 h of fermentation, KF group showed higher concentrations of formic acid, lactate and propionate than LC and MCS groups, whereas MCS group showed higher concentrations of acetate and butyrate than LC and KF groups (P < 0.05).
32737882	7	33	theme	MCS	1099:1101	arg1	group					1103:1107	MCS group	1099:1107	MCS group	1099:1107	At 36 h of fermentation, MCS group showed higher concentrations of formic acid and lactate than LC and KF groups, whereas KF group showed higher concentrations of propionate and butyrate than LC and MCS groups (P < 0.05).
32737882	13	34	theme	different	2260:2268	arg1	properties					2286:2295	different physicochemical properties	2260:2295	different physicochemical properties	2260:2295	In addition, the fermentation of DFs with different physicochemical properties had divergent impacts on microbial composition and SCFA production.
32737882	12	35	theme	fast	2088:2091	arg1	fiber					2106:2110	a fast fermentation fiber	2086:2110	a fast fermentation fiber which could produce propionate and butyrate rapidly, whereas LC is difficult to be fermented by bacteria	2086:2215	CONCLUSION Collectively, the present study revealed that KF is a fast fermentation fiber which could produce propionate and butyrate rapidly, whereas LC is difficult to be fermented by bacteria.
32737882	12	35	theme	fast	2088:2091	arg1	KF					2080:2081	KF	2080:2081	KF	2080:2081	CONCLUSION Collectively, the present study revealed that KF is a fast fermentation fiber which could produce propionate and butyrate rapidly, whereas LC is difficult to be fermented by bacteria.
32737882	2	36	theme	varying	364:370	arg1	properties					388:397	varying physicochemical properties	364:397	varying physicochemical properties (bulky, viscous, and fermentable)	364:431	The aim of the current study was to assess the effects of DFs with varying physicochemical properties (bulky, viscous, and fermentable) on fermentation kinetics and microbial composition during in vitro fermentation by fecal inoculum from lactating sow.
32737882	7	37	theme	fermentation	1085:1096	arg1	36 h					1077:1080	36 h	1077:1080	36 h of fermentation	1077:1096	At 36 h of fermentation, MCS group showed higher concentrations of formic acid and lactate than LC and KF groups, whereas KF group showed higher concentrations of propionate and butyrate than LC and MCS groups (P < 0.05).
32737882	1	38	theme	animal	282:287	arg1	health					289:294	animal health	282:294	animal health	282:294	BACKGROUND Efficient utilization of dietary fibers (DFs) is important for optimizing feed resource utilization and animal health.
32737882	7	39	theme	higher	1212:1217	arg1	concentrations					1219:1232	higher concentrations	1212:1232	higher concentrations of propionate and butyrate	1212:1259	At 36 h of fermentation, MCS group showed higher concentrations of formic acid and lactate than LC and KF groups, whereas KF group showed higher concentrations of propionate and butyrate than LC and MCS groups (P < 0.05).
32737882	12	40	theme	present	2052:2058	arg1	study					2060:2064	the present study	2048:2064	the present study	2048:2064	CONCLUSION Collectively, the present study revealed that KF is a fast fermentation fiber which could produce propionate and butyrate rapidly, whereas LC is difficult to be fermented by bacteria.
32737882	0	41	theme	dietary	11:17	arg1	fibers					19:24	dietary fibers	11:24	dietary fibers with different physicochemical properties	11:66	Effects of dietary fibers with different physicochemical properties on fermentation kinetics and microbial composition by fecal inoculum from lactating sows in vitro.
32737882	4	42	theme	Gas	770:772	arg1	acids					804:808	short-chain fatty acids	786:808	short-chain fatty acids (SCFAs)	786:816	Gas production, short-chain fatty acids (SCFAs) profiles and microbial composition were monitored during the fermentation.
32737882	4	42	theme	Gas	770:772	arg1	production					774:783	Gas production	770:783	Gas production	770:783	Gas production, short-chain fatty acids (SCFAs) profiles and microbial composition were monitored during the fermentation.
32737882	8	43	dep	showed	1330:1335	arg1	whereas					1422:1428	whereas	1422:1428	whereas	1422:1428	At 72 h of fermentation, KF group showed higher concentrations of formic acid, lactate and propionate than LC and MCS groups, whereas MCS group showed higher concentrations of acetate and butyrate than LC and KF groups (P < 0.05).
32737882	14	44	theme	lactating	2542:2550	arg1	sows					2552:2555	lactating sows	2542:2555	lactating sows	2542:2555	These findings deepen our understanding of the mechanisms of interaction between DFs and intestinal microbiota, and provide new ideas for the rational use of fiber resources in lactating sows.
32737882	2	45	theme	fermentation	436:447	arg1	kinetics					449:456	fermentation kinetics	436:456	fermentation kinetics	436:456	The aim of the current study was to assess the effects of DFs with varying physicochemical properties (bulky, viscous, and fermentable) on fermentation kinetics and microbial composition during in vitro fermentation by fecal inoculum from lactating sow.
32737882	8	46	theme	MCS	1430:1432	arg1	group					1434:1438	MCS group	1430:1438	MCS group	1430:1438	At 72 h of fermentation, KF group showed higher concentrations of formic acid, lactate and propionate than LC and MCS groups, whereas MCS group showed higher concentrations of acetate and butyrate than LC and KF groups (P < 0.05).
32737882	0	47	theme	physicochemical	41:55	arg1	properties					57:66	different physicochemical properties	31:66	different physicochemical properties	31:66	Effects of dietary fibers with different physicochemical properties on fermentation kinetics and microbial composition by fecal inoculum from lactating sows in vitro.
32737882	6	48	theme	=	1056:1056	arg1	P < 0.001					1062:1070	P < 0.001	1062:1070	P < 0.001	1062:1070	The halftime of asymptotic gas production ranked as: KF < MCS = LC (P < 0.001).
32737882	6	48	theme	=	1056:1056	arg1	LC					1058:1059	= LC	1056:1059	KF < MCS = LC (P < 0.001)	1047:1071	The halftime of asymptotic gas production ranked as: KF < MCS = LC (P < 0.001).
32737882	10	49	theme	Fibrobacter	1731:1741	arg1	higher					1759:1764	higher	1759:1764	higher	1759:1764	At 72 h of fermentation, the abundance of Fibrobacter in LC group was higher than that in MCS and KF groups.
32737882	10	49	theme	Fibrobacter	1731:1741	arg1	abundance					1718:1726	the abundance	1714:1726	the abundance of Fibrobacter in LC group	1714:1753	At 72 h of fermentation, the abundance of Fibrobacter in LC group was higher than that in MCS and KF groups.
32737882	6	50	dep	KF < MCS	1047:1054	arg1	P < 0.001					1062:1070	P < 0.001	1062:1070	P < 0.001	1062:1070	The halftime of asymptotic gas production ranked as: KF < MCS = LC (P < 0.001).
32737882	6	50	dep	KF < MCS	1047:1054	arg1	LC					1058:1059	= LC	1056:1059	KF < MCS = LC (P < 0.001)	1047:1071	The halftime of asymptotic gas production ranked as: KF < MCS = LC (P < 0.001).
32737882	8	51	theme	KF	1505:1506	arg1	P < 0.05					1516:1523	P < 0.05	1516:1523	P < 0.05	1516:1523	At 72 h of fermentation, KF group showed higher concentrations of formic acid, lactate and propionate than LC and MCS groups, whereas MCS group showed higher concentrations of acetate and butyrate than LC and KF groups (P < 0.05).
32737882	8	51	theme	KF	1505:1506	arg1	groups					1508:1513	KF groups	1505:1513	KF groups (P < 0.05)	1505:1524	At 72 h of fermentation, KF group showed higher concentrations of formic acid, lactate and propionate than LC and MCS groups, whereas MCS group showed higher concentrations of acetate and butyrate than LC and KF groups (P < 0.05).
32737882	2	52	with	DFs	355:357	arg1	properties					388:397	varying physicochemical properties	364:397	varying physicochemical properties (bulky, viscous, and fermentable)	364:431	The aim of the current study was to assess the effects of DFs with varying physicochemical properties (bulky, viscous, and fermentable) on fermentation kinetics and microbial composition during in vitro fermentation by fecal inoculum from lactating sow.
32737882	11	53	dep	bacteria	1868:1875	arg1	Anaerovibrio					1878:1889	Anaerovibrio	1878:1889	Anaerovibrio	1878:1889	In addition, we also observed that the abundances of certain specific bacteria (Anaerovibrio and Erysipelatoclostridium) were closely related to the SCFAs production (propionate and butyrate) at different fermentation times.
32737882	11	53	dep	bacteria	1868:1875	arg1	bacteria					1868:1875	certain specific bacteria	1851:1875	certain specific bacteria (Anaerovibrio and Erysipelatoclostridium)	1851:1917	In addition, we also observed that the abundances of certain specific bacteria (Anaerovibrio and Erysipelatoclostridium) were closely related to the SCFAs production (propionate and butyrate) at different fermentation times.
32737882	11	53	dep	bacteria	1868:1875	arg1	Erysipelatoclostridium					1895:1916	Erysipelatoclostridium	1895:1916	Erysipelatoclostridium	1895:1916	In addition, we also observed that the abundances of certain specific bacteria (Anaerovibrio and Erysipelatoclostridium) were closely related to the SCFAs production (propionate and butyrate) at different fermentation times.
32737882	3	54	theme	cassava	647:653	arg1	fiber					750:754	viscous fiber	742:754	viscous fiber	742:754	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	54	theme	cassava	647:653	arg1	fiber					713:717	bulky fiber	707:717	bulky fiber	707:717	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	54	theme	cassava	647:653	arg1	MCS					663:665	MCS	663:665	MCS	663:665	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	54	theme	cassava	647:653	arg1	fiber					732:736	fermentable fiber	720:736	fermentable fiber	720:736	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	54	theme	cassava	647:653	arg1	DFs					612:614	three different DFs	596:614	three different DFs	596:614	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	54	theme	cassava	647:653	arg1	lignocellulose					617:630	lignocellulose	617:630	lignocellulose (LC)	617:635	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	54	theme	cassava	647:653	arg1	starch					655:660	modified cassava starch	638:660	modified cassava starch (MCS)	638:666	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	54	theme	cassava	647:653	arg1	flour					679:683	konjac flour	672:683	konjac flour (KF)	672:688	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	8	55	theme	fermentation	1307:1318	arg1	72 h					1299:1302	72 h	1299:1302	72 h of fermentation	1299:1318	At 72 h of fermentation, KF group showed higher concentrations of formic acid, lactate and propionate than LC and MCS groups, whereas MCS group showed higher concentrations of acetate and butyrate than LC and KF groups (P < 0.05).
32737882	2	56	theme	DFs	355:357	arg1	effects					344:350	the effects	340:350	the effects of DFs with varying physicochemical properties (bulky, viscous, and fermentable) on fermentation kinetics and microbial composition	340:482	The aim of the current study was to assess the effects of DFs with varying physicochemical properties (bulky, viscous, and fermentable) on fermentation kinetics and microbial composition during in vitro fermentation by fecal inoculum from lactating sow.
32737882	11	57	theme	fermentation	2003:2014	arg1	times					2016:2020	different fermentation times	1993:2020	different fermentation times	1993:2020	In addition, we also observed that the abundances of certain specific bacteria (Anaerovibrio and Erysipelatoclostridium) were closely related to the SCFAs production (propionate and butyrate) at different fermentation times.
32737882	14	58	theme	rational	2507:2514	arg1	use					2516:2518	the rational use	2503:2518	the rational use of fiber resources in lactating sows	2503:2555	These findings deepen our understanding of the mechanisms of interaction between DFs and intestinal microbiota, and provide new ideas for the rational use of fiber resources in lactating sows.
32737882	8	59	theme	acetate	1472:1478	arg1	concentrations					1454:1467	higher concentrations	1447:1467	higher concentrations of acetate and butyrate	1447:1491	At 72 h of fermentation, KF group showed higher concentrations of formic acid, lactate and propionate than LC and MCS groups, whereas MCS group showed higher concentrations of acetate and butyrate than LC and KF groups (P < 0.05).
32737882	3	60	theme	konjac	672:677	arg1	fiber					750:754	viscous fiber	742:754	viscous fiber	742:754	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	60	theme	konjac	672:677	arg1	KF					686:687	KF	686:687	KF	686:687	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	60	theme	konjac	672:677	arg1	fiber					713:717	bulky fiber	707:717	bulky fiber	707:717	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	60	theme	konjac	672:677	arg1	fiber					732:736	fermentable fiber	720:736	fermentable fiber	720:736	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	60	theme	konjac	672:677	arg1	DFs					612:614	three different DFs	596:614	three different DFs	596:614	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	60	theme	konjac	672:677	arg1	lignocellulose					617:630	lignocellulose	617:630	lignocellulose (LC)	617:635	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	60	theme	konjac	672:677	arg1	starch					655:660	modified cassava starch	638:660	modified cassava starch (MCS)	638:666	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	60	theme	konjac	672:677	arg1	flour					679:683	konjac flour	672:683	konjac flour (KF)	672:688	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	1	61	theme	feed	252:255	arg1	utilization					266:276	feed resource utilization	252:276	feed resource utilization	252:276	BACKGROUND Efficient utilization of dietary fibers (DFs) is important for optimizing feed resource utilization and animal health.
32737882	11	62	theme	SCFAs	1947:1951	arg1	production					1953:1962	the SCFAs production	1943:1962	the SCFAs production	1943:1962	In addition, we also observed that the abundances of certain specific bacteria (Anaerovibrio and Erysipelatoclostridium) were closely related to the SCFAs production (propionate and butyrate) at different fermentation times.
32737882	6	63	theme	gas	1021:1023	arg1	production					1025:1034	asymptotic gas production	1010:1034	asymptotic gas production	1010:1034	The halftime of asymptotic gas production ranked as: KF < MCS = LC (P < 0.001).
32737882	8	64	theme	butyrate	1484:1491	arg1	concentrations					1454:1467	higher concentrations	1447:1467	higher concentrations of acetate and butyrate	1447:1491	At 72 h of fermentation, KF group showed higher concentrations of formic acid, lactate and propionate than LC and MCS groups, whereas MCS group showed higher concentrations of acetate and butyrate than LC and KF groups (P < 0.05).
32737882	10	65	theme	LC	1746:1747	arg1	group					1749:1753	LC group	1746:1753	LC group	1746:1753	At 72 h of fermentation, the abundance of Fibrobacter in LC group was higher than that in MCS and KF groups.
32737882	10	66	from	abundance	1718:1726	arg1	group					1749:1753	LC group	1746:1753	LC group	1746:1753	At 72 h of fermentation, the abundance of Fibrobacter in LC group was higher than that in MCS and KF groups.
32737882	1	67	theme	Efficient	178:186	arg1	utilization					188:198	BACKGROUND Efficient utilization	167:198	BACKGROUND Efficient utilization of dietary fibers (DFs)	167:222	BACKGROUND Efficient utilization of dietary fibers (DFs) is important for optimizing feed resource utilization and animal health.
32737882	4	68	theme	short-chain	786:796	arg1	acids					804:808	short-chain fatty acids	786:808	short-chain fatty acids (SCFAs)	786:816	Gas production, short-chain fatty acids (SCFAs) profiles and microbial composition were monitored during the fermentation.
32737882	4	68	theme	short-chain	786:796	arg1	production					774:783	Gas production	770:783	Gas production	770:783	Gas production, short-chain fatty acids (SCFAs) profiles and microbial composition were monitored during the fermentation.
32737882	4	68	theme	short-chain	786:796	arg1	SCFAs					811:815	SCFAs	811:815	SCFAs	811:815	Gas production, short-chain fatty acids (SCFAs) profiles and microbial composition were monitored during the fermentation.
32737882	2	69	from	effects	344:350	arg1	kinetics					449:456	fermentation kinetics	436:456	fermentation kinetics	436:456	The aim of the current study was to assess the effects of DFs with varying physicochemical properties (bulky, viscous, and fermentable) on fermentation kinetics and microbial composition during in vitro fermentation by fecal inoculum from lactating sow.
32737882	2	69	from	effects	344:350	arg1	composition					472:482	microbial composition	462:482	microbial composition	462:482	The aim of the current study was to assess the effects of DFs with varying physicochemical properties (bulky, viscous, and fermentable) on fermentation kinetics and microbial composition during in vitro fermentation by fecal inoculum from lactating sow.
32737882	11	70	dep	related	1932:1938	arg1	butyrate					1980:1987	butyrate	1980:1987	butyrate	1980:1987	In addition, we also observed that the abundances of certain specific bacteria (Anaerovibrio and Erysipelatoclostridium) were closely related to the SCFAs production (propionate and butyrate) at different fermentation times.
32737882	11	70	dep	related	1932:1938	arg1	propionate					1965:1974	propionate	1965:1974	propionate	1965:1974	In addition, we also observed that the abundances of certain specific bacteria (Anaerovibrio and Erysipelatoclostridium) were closely related to the SCFAs production (propionate and butyrate) at different fermentation times.
32737882	11	70	dep	related	1932:1938	arg1	related					1932:1938	related	1932:1938	related	1932:1938	In addition, we also observed that the abundances of certain specific bacteria (Anaerovibrio and Erysipelatoclostridium) were closely related to the SCFAs production (propionate and butyrate) at different fermentation times.
32737882	11	70	dep	related	1932:1938	arg1	abundances					1837:1846	the abundances	1833:1846	the abundances of certain specific bacteria (Anaerovibrio and Erysipelatoclostridium)	1833:1917	In addition, we also observed that the abundances of certain specific bacteria (Anaerovibrio and Erysipelatoclostridium) were closely related to the SCFAs production (propionate and butyrate) at different fermentation times.
32737882	2	71	theme	current	312:318	arg1	study					320:324	the current study	308:324	the current study	308:324	The aim of the current study was to assess the effects of DFs with varying physicochemical properties (bulky, viscous, and fermentable) on fermentation kinetics and microbial composition during in vitro fermentation by fecal inoculum from lactating sow.
32737882	13	72	with	fermentation	2235:2246	arg1	properties					2286:2295	different physicochemical properties	2260:2295	different physicochemical properties	2260:2295	In addition, the fermentation of DFs with different physicochemical properties had divergent impacts on microbial composition and SCFA production.
32737882	3	73	theme	bulky	707:711	arg1	DFs					612:614	three different DFs	596:614	three different DFs	596:614	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	73	theme	bulky	707:711	arg1	starch					655:660	modified cassava starch	638:660	modified cassava starch (MCS)	638:666	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	73	theme	bulky	707:711	arg1	flour					679:683	konjac flour	672:683	konjac flour (KF)	672:688	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	73	theme	bulky	707:711	arg1	fiber					713:717	bulky fiber	707:717	bulky fiber	707:717	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	73	theme	bulky	707:711	arg1	lignocellulose					617:630	lignocellulose	617:630	lignocellulose (LC)	617:635	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	1	74	theme	fibers	211:216	arg1	utilization					188:198	BACKGROUND Efficient utilization	167:198	BACKGROUND Efficient utilization of dietary fibers (DFs)	167:222	BACKGROUND Efficient utilization of dietary fibers (DFs) is important for optimizing feed resource utilization and animal health.
32737882	2	75	theme	lactating	536:544	arg1	sow					546:548	lactating sow	536:548	lactating sow	536:548	The aim of the current study was to assess the effects of DFs with varying physicochemical properties (bulky, viscous, and fermentable) on fermentation kinetics and microbial composition during in vitro fermentation by fecal inoculum from lactating sow.
32737882	8	76	theme	KF	1321:1322	arg1	group					1324:1328	KF group	1321:1328	KF group	1321:1328	At 72 h of fermentation, KF group showed higher concentrations of formic acid, lactate and propionate than LC and MCS groups, whereas MCS group showed higher concentrations of acetate and butyrate than LC and KF groups (P < 0.05).
32737882	14	77	theme	resources	2529:2537	arg1	use					2516:2518	the rational use	2503:2518	the rational use of fiber resources in lactating sows	2503:2555	These findings deepen our understanding of the mechanisms of interaction between DFs and intestinal microbiota, and provide new ideas for the rational use of fiber resources in lactating sows.
32737882	13	78	theme	microbial	2322:2330	arg1	composition					2332:2342	microbial composition	2322:2342	microbial composition	2322:2342	In addition, the fermentation of DFs with different physicochemical properties had divergent impacts on microbial composition and SCFA production.
32737882	9	79	theme	Anaerovibrio	1552:1563	arg1	abundances					1592:1601	Anaerovibrio and Erysipelatoclostridium abundances	1552:1601	Anaerovibrio and Erysipelatoclostridium abundances	1552:1601	At 36 h of fermentation, Anaerovibrio and Erysipelatoclostridium abundances were higher in KF group, whereas Proteiniclasticum abundance was higher in MCS group.
32737882	13	80	theme	physicochemical	2270:2284	arg1	properties					2286:2295	different physicochemical properties	2260:2295	different physicochemical properties	2260:2295	In addition, the fermentation of DFs with different physicochemical properties had divergent impacts on microbial composition and SCFA production.
32737882	3	81	theme	physicochemical	568:582	arg1	properties					584:593	the physicochemical properties	564:593	the physicochemical properties	564:593	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	0	82	from	Effects	0:6	arg1	kinetics					84:91	fermentation kinetics	71:91	fermentation kinetics	71:91	Effects of dietary fibers with different physicochemical properties on fermentation kinetics and microbial composition by fecal inoculum from lactating sows in vitro.
32737882	0	82	from	Effects	0:6	arg1	composition					107:117	microbial composition	97:117	microbial composition by fecal inoculum from lactating sows in vitro	97:164	Effects of dietary fibers with different physicochemical properties on fermentation kinetics and microbial composition by fecal inoculum from lactating sows in vitro.
32737882	14	83	theme	intestinal	2454:2463	arg1	microbiota					2465:2474	intestinal microbiota	2454:2474	intestinal microbiota	2454:2474	These findings deepen our understanding of the mechanisms of interaction between DFs and intestinal microbiota, and provide new ideas for the rational use of fiber resources in lactating sows.
32737882	9	84	theme	Erysipelatoclostridium	1569:1590	arg1	abundances					1592:1601	Anaerovibrio and Erysipelatoclostridium abundances	1552:1601	Anaerovibrio and Erysipelatoclostridium abundances	1552:1601	At 36 h of fermentation, Anaerovibrio and Erysipelatoclostridium abundances were higher in KF group, whereas Proteiniclasticum abundance was higher in MCS group.
32737882	8	85	theme	formic	1362:1367	arg1	acid					1369:1372	formic acid	1362:1372	formic acid	1362:1372	At 72 h of fermentation, KF group showed higher concentrations of formic acid, lactate and propionate than LC and MCS groups, whereas MCS group showed higher concentrations of acetate and butyrate than LC and KF groups (P < 0.05).
32737882	0	86	theme	fermentation	71:82	arg1	kinetics					84:91	fermentation kinetics	71:91	fermentation kinetics	71:91	Effects of dietary fibers with different physicochemical properties on fermentation kinetics and microbial composition by fecal inoculum from lactating sows in vitro.
32737882	3	87	theme	different	602:610	arg1	fiber					750:754	viscous fiber	742:754	viscous fiber	742:754	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	87	theme	different	602:610	arg1	fiber					713:717	bulky fiber	707:717	bulky fiber	707:717	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	87	theme	different	602:610	arg1	fiber					732:736	fermentable fiber	720:736	fermentable fiber	720:736	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	87	theme	different	602:610	arg1	DFs					612:614	three different DFs	596:614	three different DFs	596:614	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	87	theme	different	602:610	arg1	lignocellulose					617:630	lignocellulose	617:630	lignocellulose (LC)	617:635	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	87	theme	different	602:610	arg1	starch					655:660	modified cassava starch	638:660	modified cassava starch (MCS)	638:666	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	87	theme	different	602:610	arg1	flour					679:683	konjac flour	672:683	konjac flour (KF)	672:688	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	12	88	dep	CONCLUSION	2023:2032	arg1	revealed					2066:2073	revealed	2066:2073	revealed that KF is a fast fermentation fiber which could produce propionate and butyrate rapidly, whereas LC is difficult to be fermented by bacteria	2066:2215	CONCLUSION Collectively, the present study revealed that KF is a fast fermentation fiber which could produce propionate and butyrate rapidly, whereas LC is difficult to be fermented by bacteria.
32737882	14	89	theme	interaction	2426:2436	arg1	mechanisms					2412:2421	the mechanisms	2408:2421	the mechanisms of interaction between DFs and intestinal microbiota	2408:2474	These findings deepen our understanding of the mechanisms of interaction between DFs and intestinal microbiota, and provide new ideas for the rational use of fiber resources in lactating sows.
32737882	3	90	theme	viscous	742:748	arg1	fiber					750:754	viscous fiber	742:754	viscous fiber	742:754	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	90	theme	viscous	742:748	arg1	DFs					612:614	three different DFs	596:614	three different DFs	596:614	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	90	theme	viscous	742:748	arg1	starch					655:660	modified cassava starch	638:660	modified cassava starch (MCS)	638:666	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	90	theme	viscous	742:748	arg1	flour					679:683	konjac flour	672:683	konjac flour (KF)	672:688	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	90	theme	viscous	742:748	arg1	lignocellulose					617:630	lignocellulose	617:630	lignocellulose (LC)	617:635	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	7	91	theme	higher	1116:1121	arg1	concentrations					1123:1136	higher concentrations	1116:1136	higher concentrations of formic acid and lactate	1116:1163	At 36 h of fermentation, MCS group showed higher concentrations of formic acid and lactate than LC and KF groups, whereas KF group showed higher concentrations of propionate and butyrate than LC and MCS groups (P < 0.05).
32737882	14	92	theme	mechanisms	2412:2421	arg1	understanding					2391:2403	our understanding	2387:2403	our understanding of the mechanisms of interaction between DFs and intestinal microbiota	2387:2474	These findings deepen our understanding of the mechanisms of interaction between DFs and intestinal microbiota, and provide new ideas for the rational use of fiber resources in lactating sows.
32737882	2	93	theme	microbial	462:470	arg1	composition					472:482	microbial composition	462:482	microbial composition	462:482	The aim of the current study was to assess the effects of DFs with varying physicochemical properties (bulky, viscous, and fermentable) on fermentation kinetics and microbial composition during in vitro fermentation by fecal inoculum from lactating sow.
32737882	14	94	from	use	2516:2518	arg1	sows					2552:2555	lactating sows	2542:2555	lactating sows	2542:2555	These findings deepen our understanding of the mechanisms of interaction between DFs and intestinal microbiota, and provide new ideas for the rational use of fiber resources in lactating sows.
32737882	13	95	theme	DFs	2251:2253	arg1	fermentation					2235:2246	the fermentation	2231:2246	the fermentation of DFs with different physicochemical properties	2231:2295	In addition, the fermentation of DFs with different physicochemical properties had divergent impacts on microbial composition and SCFA production.
32737882	0	96	theme	fecal	122:126	arg1	inoculum					128:135	fecal inoculum	122:135	fecal inoculum from lactating sows in vitro	122:164	Effects of dietary fibers with different physicochemical properties on fermentation kinetics and microbial composition by fecal inoculum from lactating sows in vitro.
32737882	6	97	theme	asymptotic	1010:1019	arg1	production					1025:1034	asymptotic gas production	1010:1034	asymptotic gas production	1010:1034	The halftime of asymptotic gas production ranked as: KF < MCS = LC (P < 0.001).
32737882	12	98	theme	fermentation	2093:2104	arg1	fiber					2106:2110	a fast fermentation fiber	2086:2110	a fast fermentation fiber which could produce propionate and butyrate rapidly, whereas LC is difficult to be fermented by bacteria	2086:2215	CONCLUSION Collectively, the present study revealed that KF is a fast fermentation fiber which could produce propionate and butyrate rapidly, whereas LC is difficult to be fermented by bacteria.
32737882	12	98	theme	fermentation	2093:2104	arg1	KF					2080:2081	KF	2080:2081	KF	2080:2081	CONCLUSION Collectively, the present study revealed that KF is a fast fermentation fiber which could produce propionate and butyrate rapidly, whereas LC is difficult to be fermented by bacteria.
32737882	2	99	theme	physicochemical	372:386	arg1	properties					388:397	varying physicochemical properties	364:397	varying physicochemical properties (bulky, viscous, and fermentable)	364:431	The aim of the current study was to assess the effects of DFs with varying physicochemical properties (bulky, viscous, and fermentable) on fermentation kinetics and microbial composition during in vitro fermentation by fecal inoculum from lactating sow.
32737882	11	100	theme	different	1993:2001	arg1	times					2016:2020	different fermentation times	1993:2020	different fermentation times	1993:2020	In addition, we also observed that the abundances of certain specific bacteria (Anaerovibrio and Erysipelatoclostridium) were closely related to the SCFAs production (propionate and butyrate) at different fermentation times.
32737882	0	101	theme	fibers	19:24	arg1	Effects					0:6	Effects	0:6	Effects of dietary fibers with different physicochemical properties on fermentation kinetics and microbial composition by fecal inoculum from lactating sows in vitro.	0:165	Effects of dietary fibers with different physicochemical properties on fermentation kinetics and microbial composition by fecal inoculum from lactating sows in vitro.
32737882	2	102	dep	properties	388:397	arg1	fermentable					420:430	fermentable	420:430	fermentable	420:430	The aim of the current study was to assess the effects of DFs with varying physicochemical properties (bulky, viscous, and fermentable) on fermentation kinetics and microbial composition during in vitro fermentation by fecal inoculum from lactating sow.
32737882	2	102	dep	properties	388:397	arg1	bulky					400:404	bulky	400:404	bulky	400:404	The aim of the current study was to assess the effects of DFs with varying physicochemical properties (bulky, viscous, and fermentable) on fermentation kinetics and microbial composition during in vitro fermentation by fecal inoculum from lactating sow.
32737882	2	102	dep	properties	388:397	arg1	viscous					407:413	viscous	407:413	viscous	407:413	The aim of the current study was to assess the effects of DFs with varying physicochemical properties (bulky, viscous, and fermentable) on fermentation kinetics and microbial composition during in vitro fermentation by fecal inoculum from lactating sow.
32737882	7	103	theme	KF	1196:1197	arg1	group					1199:1203	KF group	1196:1203	KF group	1196:1203	At 36 h of fermentation, MCS group showed higher concentrations of formic acid and lactate than LC and KF groups, whereas KF group showed higher concentrations of propionate and butyrate than LC and MCS groups (P < 0.05).
32737882	9	104	theme	MCS	1678:1680	arg1	group					1682:1686	MCS group	1678:1686	MCS group	1678:1686	At 36 h of fermentation, Anaerovibrio and Erysipelatoclostridium abundances were higher in KF group, whereas Proteiniclasticum abundance was higher in MCS group.
32737882	13	105	contain	had	2297:2299	arg1	fermentation					2235:2246	the fermentation	2231:2246	the fermentation of DFs with different physicochemical properties	2231:2295	In addition, the fermentation of DFs with different physicochemical properties had divergent impacts on microbial composition and SCFA production.
32737882	13	105	contain	had	2297:2299	arg2	impacts					2311:2317	divergent impacts	2301:2317	divergent impacts	2301:2317	In addition, the fermentation of DFs with different physicochemical properties had divergent impacts on microbial composition and SCFA production.
32737882	0	106	theme	different	31:39	arg1	properties					57:66	different physicochemical properties	31:66	different physicochemical properties	31:66	Effects of dietary fibers with different physicochemical properties on fermentation kinetics and microbial composition by fecal inoculum from lactating sows in vitro.
32737882	10	107	theme	fermentation	1700:1711	arg1	72 h					1692:1695	72 h	1692:1695	72 h of fermentation	1692:1711	At 72 h of fermentation, the abundance of Fibrobacter in LC group was higher than that in MCS and KF groups.
32737882	0	108	with	fibers	19:24	arg1	properties					57:66	different physicochemical properties	31:66	different physicochemical properties	31:66	Effects of dietary fibers with different physicochemical properties on fermentation kinetics and microbial composition by fecal inoculum from lactating sows in vitro.
32737882	7	109	theme	KF	1177:1178	arg1	groups					1180:1185	KF groups	1177:1185	KF groups	1177:1185	At 36 h of fermentation, MCS group showed higher concentrations of formic acid and lactate than LC and KF groups, whereas KF group showed higher concentrations of propionate and butyrate than LC and MCS groups (P < 0.05).
32737882	8	110	theme	higher	1447:1452	arg1	concentrations					1454:1467	higher concentrations	1447:1467	higher concentrations of acetate and butyrate	1447:1491	At 72 h of fermentation, KF group showed higher concentrations of formic acid, lactate and propionate than LC and MCS groups, whereas MCS group showed higher concentrations of acetate and butyrate than LC and KF groups (P < 0.05).
32737882	4	111	theme	production	774:783	arg1	profiles					818:825	Gas production, short-chain fatty acids (SCFAs) profiles	770:825	Gas production, short-chain fatty acids (SCFAs) profiles	770:825	Gas production, short-chain fatty acids (SCFAs) profiles and microbial composition were monitored during the fermentation.
32737882	2	112	dep	in	491:492	arg1	vitro					494:498	vitro	494:498	vitro	494:498	The aim of the current study was to assess the effects of DFs with varying physicochemical properties (bulky, viscous, and fermentable) on fermentation kinetics and microbial composition during in vitro fermentation by fecal inoculum from lactating sow.
32737882	14	113	theme	fiber	2523:2527	arg1	resources					2529:2537	fiber resources	2523:2537	fiber resources	2523:2537	These findings deepen our understanding of the mechanisms of interaction between DFs and intestinal microbiota, and provide new ideas for the rational use of fiber resources in lactating sows.
32737882	0	114	from	sows	152:155	arg1	vitro					160:164	vitro	160:164	vitro	160:164	Effects of dietary fibers with different physicochemical properties on fermentation kinetics and microbial composition by fecal inoculum from lactating sows in vitro.
32737882	0	114	from	sows	152:155	arg1	inoculum					128:135	fecal inoculum	122:135	fecal inoculum from lactating sows in vitro	122:164	Effects of dietary fibers with different physicochemical properties on fermentation kinetics and microbial composition by fecal inoculum from lactating sows in vitro.
32737882	7	115	dep	showed	1109:1114	arg1	whereas					1188:1194	whereas	1188:1194	whereas	1188:1194	At 36 h of fermentation, MCS group showed higher concentrations of formic acid and lactate than LC and KF groups, whereas KF group showed higher concentrations of propionate and butyrate than LC and MCS groups (P < 0.05).
32737882	3	116	theme	modified	638:645	arg1	fiber					750:754	viscous fiber	742:754	viscous fiber	742:754	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	116	theme	modified	638:645	arg1	fiber					713:717	bulky fiber	707:717	bulky fiber	707:717	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	116	theme	modified	638:645	arg1	MCS					663:665	MCS	663:665	MCS	663:665	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	116	theme	modified	638:645	arg1	fiber					732:736	fermentable fiber	720:736	fermentable fiber	720:736	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	116	theme	modified	638:645	arg1	DFs					612:614	three different DFs	596:614	three different DFs	596:614	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	116	theme	modified	638:645	arg1	lignocellulose					617:630	lignocellulose	617:630	lignocellulose (LC)	617:635	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	116	theme	modified	638:645	arg1	starch					655:660	modified cassava starch	638:660	modified cassava starch (MCS)	638:666	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	3	116	theme	modified	638:645	arg1	flour					679:683	konjac flour	672:683	konjac flour (KF)	672:688	According to the physicochemical properties, three different DFs, lignocellulose (LC), modified cassava starch (MCS) and konjac flour (KF) were selected as bulky fiber, fermentable fiber and viscous fiber respectively.
32737882	2	117	theme	study	320:324	arg1	aim					301:303	The aim	297:303	The aim of the current study	297:324	The aim of the current study was to assess the effects of DFs with varying physicochemical properties (bulky, viscous, and fermentable) on fermentation kinetics and microbial composition during in vitro fermentation by fecal inoculum from lactating sow.
32737882	8	118	dep	LC	1403:1404	arg1	groups					1414:1419	groups	1414:1419	groups	1414:1419	At 72 h of fermentation, KF group showed higher concentrations of formic acid, lactate and propionate than LC and MCS groups, whereas MCS group showed higher concentrations of acetate and butyrate than LC and KF groups (P < 0.05).
32737882	7	119	theme	lactate	1157:1163	arg1	concentrations					1123:1136	higher concentrations	1116:1136	higher concentrations of formic acid and lactate	1116:1163	At 36 h of fermentation, MCS group showed higher concentrations of formic acid and lactate than LC and KF groups, whereas KF group showed higher concentrations of propionate and butyrate than LC and MCS groups (P < 0.05).
32737882	2	120	from	sow	546:548	arg1	inoculum					522:529	fecal inoculum	516:529	fecal inoculum from lactating sow	516:548	The aim of the current study was to assess the effects of DFs with varying physicochemical properties (bulky, viscous, and fermentable) on fermentation kinetics and microbial composition during in vitro fermentation by fecal inoculum from lactating sow.
32737882	5	121	from	production	929:938	arg1	GP72h					949:953	GP72h	949:953	GP72h	949:953	RESULTS Results showed that the gas production in 72 h (GP72h ) ranked as: KF > MCS > LC (P < 0.05).
32737882	5	121	from	production	929:938	arg1	72 h					943:946	72 h	943:946	72 h (GP72h )	943:955	RESULTS Results showed that the gas production in 72 h (GP72h ) ranked as: KF > MCS > LC (P < 0.05).
32679332	6	0	theme	maximal	1161:1167	arg1	absorption					1169:1178	maximal absorption	1161:1178	maximal absorption (~99%)	1161:1185	The results identified the best performing variants and the optimal conditions for maximal absorption (~99%).
32679332	6	0	theme	maximal	1161:1167	arg1	%					1184:1184	~99%	1181:1184	~99%	1181:1184	The results identified the best performing variants and the optimal conditions for maximal absorption (~99%).
32679332	3	1	theme	X-Ray	601:605	arg1	XRD					620:622	XRD	620:622	XRD	620:622	Synthesis of NIONs was confirmed by Transmission electron microscopy (TEM), X-Ray Diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopic (XPS).
32679332	3	1	theme	X-Ray	601:605	arg1	Diffraction					607:617	X-Ray Diffraction	601:617	X-Ray Diffraction (XRD)	601:623	Synthesis of NIONs was confirmed by Transmission electron microscopy (TEM), X-Ray Diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopic (XPS).
32679332	3	1	theme	X-Ray	601:605	arg1	microscopy					583:592	Transmission electron microscopy	561:592	Transmission electron microscopy (TEM)	561:598	Synthesis of NIONs was confirmed by Transmission electron microscopy (TEM), X-Ray Diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopic (XPS).
32679332	10	2	theme	water	1661:1665	arg1	applications					1679:1690	water remediation applications	1661:1690	water remediation applications due to their high adsorptive performance, magnetic recoverability and recyclability	1661:1774	NIONs show promising commercial potential for water remediation applications due to their high adsorptive performance, magnetic recoverability and recyclability.
32679332	1	3	link	derived	298:304	arg1	nanocelluloses					306:319	rice husk and sugarcane bagasse derived nanocelluloses	266:319	rice husk and sugarcane bagasse derived nanocelluloses	266:319	Nanocellulose Iron Oxide Nanobiocomposites (NIONs) were synthesized from rice husk and sugarcane bagasse derived nanocelluloses for adsorptive removal of arsenic and associated contaminants present in groundwater samples.
32679332	7	4	theme	Network	1302:1308	arg1	model					1316:1320	a three-layer feed-forward Multilayer Perceptron (MLP) based Artificial Neural Network (ANN) model	1223:1320	a three-layer feed-forward Multilayer Perceptron (MLP) based Artificial Neural Network (ANN) model	1223:1320	These results were validated using a three-layer feed-forward Multilayer Perceptron (MLP) based Artificial Neural Network (ANN) model.
32679332	1	5	theme	adsorptive	325:334	arg1	removal					336:342	adsorptive removal	325:342	adsorptive removal of arsenic and associated contaminants present in groundwater samples	325:412	Nanocellulose Iron Oxide Nanobiocomposites (NIONs) were synthesized from rice husk and sugarcane bagasse derived nanocelluloses for adsorptive removal of arsenic and associated contaminants present in groundwater samples.
32679332	5	6	theme	central	961:967	arg1	CCD					987:989	CCD	987:989	CCD	987:989	The experimental conditions for 10 different variants were modelled using response surface methodology (RSM) based on central composite design (CCD), considering the parameters; adsorbate dosage, adsorbent dosage, pH and contact time.
32679332	5	6	theme	central	961:967	arg1	design					979:984	central composite design	961:984	central composite design (CCD)	961:990	The experimental conditions for 10 different variants were modelled using response surface methodology (RSM) based on central composite design (CCD), considering the parameters; adsorbate dosage, adsorbent dosage, pH and contact time.
32679332	1	7	attach	present	383:389	arg2	contaminants					370:381	arsenic and associated contaminants	347:381	arsenic and associated contaminants present in groundwater samples	347:412	Nanocellulose Iron Oxide Nanobiocomposites (NIONs) were synthesized from rice husk and sugarcane bagasse derived nanocelluloses for adsorptive removal of arsenic and associated contaminants present in groundwater samples.
32679332	1	7	attach	present	383:389	arg1	samples					406:412	groundwater samples	394:412	groundwater samples	394:412	Nanocellulose Iron Oxide Nanobiocomposites (NIONs) were synthesized from rice husk and sugarcane bagasse derived nanocelluloses for adsorptive removal of arsenic and associated contaminants present in groundwater samples.
32679332	9	8	theme	arsenic	1526:1532	arg1	samples					1559:1565	field-based arsenic contaminated groundwater samples	1514:1565	field-based arsenic contaminated groundwater samples	1514:1565	The standardized conditions were used to expand the study to field-based arsenic contaminated groundwater samples and their performance to commercial adsorbents.
32679332	3	9	theme	X-ray	677:681	arg1	spectroscopic					697:709	X-ray photoelectron spectroscopic	677:709	X-ray photoelectron spectroscopic (XPS)	677:715	Synthesis of NIONs was confirmed by Transmission electron microscopy (TEM), X-Ray Diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopic (XPS).
32679332	3	9	theme	X-ray	677:681	arg1	XPS					712:714	XPS	712:714	XPS	712:714	Synthesis of NIONs was confirmed by Transmission electron microscopy (TEM), X-Ray Diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopic (XPS).
32679332	9	10	theme	groundwater	1547:1557	arg1	samples					1559:1565	field-based arsenic contaminated groundwater samples	1514:1565	field-based arsenic contaminated groundwater samples	1514:1565	The standardized conditions were used to expand the study to field-based arsenic contaminated groundwater samples and their performance to commercial adsorbents.
32679332	4	11	theme	probable	792:799	arg1	mechanism					812:820	probable adsorption mechanism	792:820	probable adsorption mechanism of Arsenic by NIONs	792:840	FTIR and XPS data together with adsorption kinetics provide insights into probable adsorption mechanism of Arsenic by NIONs.
32679332	10	12	theme	high	1705:1708	arg1	performance					1721:1731	their high adsorptive performance	1699:1731	their high adsorptive performance	1699:1731	NIONs show promising commercial potential for water remediation applications due to their high adsorptive performance, magnetic recoverability and recyclability.
32679332	3	13	dep	Fourier	626:632	arg1	transform					634:642	transform	634:642	transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopic (XPS)	634:715	Synthesis of NIONs was confirmed by Transmission electron microscopy (TEM), X-Ray Diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopic (XPS).
32679332	7	14	theme	Neural	1295:1300	arg1	model					1316:1320	a three-layer feed-forward Multilayer Perceptron (MLP) based Artificial Neural Network (ANN) model	1223:1320	a three-layer feed-forward Multilayer Perceptron (MLP) based Artificial Neural Network (ANN) model	1223:1320	These results were validated using a three-layer feed-forward Multilayer Perceptron (MLP) based Artificial Neural Network (ANN) model.
32679332	1	15	theme	groundwater	394:404	arg1	samples					406:412	groundwater samples	394:412	groundwater samples	394:412	Nanocellulose Iron Oxide Nanobiocomposites (NIONs) were synthesized from rice husk and sugarcane bagasse derived nanocelluloses for adsorptive removal of arsenic and associated contaminants present in groundwater samples.
32679332	9	16	theme	commercial	1592:1601	arg1	adsorbents					1603:1612	commercial adsorbents	1592:1612	commercial adsorbents	1592:1612	The standardized conditions were used to expand the study to field-based arsenic contaminated groundwater samples and their performance to commercial adsorbents.
32679332	5	17	dep	parameters	1009:1018	arg1	dosage					1031:1036	adsorbate dosage	1021:1036	adsorbate dosage	1021:1036	The experimental conditions for 10 different variants were modelled using response surface methodology (RSM) based on central composite design (CCD), considering the parameters; adsorbate dosage, adsorbent dosage, pH and contact time.
32679332	5	17	dep	parameters	1009:1018	arg1	pH					1057:1058	pH	1057:1058	pH	1057:1058	The experimental conditions for 10 different variants were modelled using response surface methodology (RSM) based on central composite design (CCD), considering the parameters; adsorbate dosage, adsorbent dosage, pH and contact time.
32679332	5	17	dep	parameters	1009:1018	arg1	time					1072:1075	contact time	1064:1075	contact time	1064:1075	The experimental conditions for 10 different variants were modelled using response surface methodology (RSM) based on central composite design (CCD), considering the parameters; adsorbate dosage, adsorbent dosage, pH and contact time.
32679332	5	17	dep	parameters	1009:1018	arg1	dosage					1049:1054	adsorbent dosage	1039:1054	adsorbent dosage	1039:1054	The experimental conditions for 10 different variants were modelled using response surface methodology (RSM) based on central composite design (CCD), considering the parameters; adsorbate dosage, adsorbent dosage, pH and contact time.
32679332	5	17	dep	parameters	1009:1018	arg1	parameters					1009:1018	the parameters	1005:1018	the parameters; adsorbate dosage, adsorbent dosage, pH and contact time	1005:1075	The experimental conditions for 10 different variants were modelled using response surface methodology (RSM) based on central composite design (CCD), considering the parameters; adsorbate dosage, adsorbent dosage, pH and contact time.
32679332	0	18	theme	Nanocellulose	94:106	arg1	Nanobiocomposites					119:135	agrowaste-derived Nanocellulose Iron Oxide Nanobiocomposites	76:135	agrowaste-derived Nanocellulose Iron Oxide Nanobiocomposites	76:135	Modelling and optimization of factors influencing adsorptive performance of agrowaste-derived Nanocellulose Iron Oxide Nanobiocomposites during remediation of Arsenic contaminated groundwater.
32679332	1	19	theme	Oxide	212:216	arg1	NIONs					237:241	NIONs	237:241	NIONs	237:241	Nanocellulose Iron Oxide Nanobiocomposites (NIONs) were synthesized from rice husk and sugarcane bagasse derived nanocelluloses for adsorptive removal of arsenic and associated contaminants present in groundwater samples.
32679332	1	19	theme	Oxide	212:216	arg1	Nanobiocomposites					218:234	Nanocellulose Iron Oxide Nanobiocomposites	193:234	Nanocellulose Iron Oxide Nanobiocomposites (NIONs)	193:242	Nanocellulose Iron Oxide Nanobiocomposites (NIONs) were synthesized from rice husk and sugarcane bagasse derived nanocelluloses for adsorptive removal of arsenic and associated contaminants present in groundwater samples.
32679332	8	20	theme	optimized	1388:1396	arg1	conditions					1398:1407	optimized conditions	1388:1407	optimized conditions of highest adsorption by specific variants	1388:1450	Both RSM and ANN chemometric models were in close conformity for optimized conditions of highest adsorption by specific variants.
32679332	0	21	theme	Oxide	113:117	arg1	Nanobiocomposites					119:135	agrowaste-derived Nanocellulose Iron Oxide Nanobiocomposites	76:135	agrowaste-derived Nanocellulose Iron Oxide Nanobiocomposites	76:135	Modelling and optimization of factors influencing adsorptive performance of agrowaste-derived Nanocellulose Iron Oxide Nanobiocomposites during remediation of Arsenic contaminated groundwater.
32679332	8	22	dep	Both	1323:1326	arg1	RSM					1328:1330	RSM	1328:1330	RSM	1328:1330	Both RSM and ANN chemometric models were in close conformity for optimized conditions of highest adsorption by specific variants.
32679332	3	23	theme	NIONs	538:542	arg1	Synthesis					525:533	Synthesis	525:533	Synthesis of NIONs	525:542	Synthesis of NIONs was confirmed by Transmission electron microscopy (TEM), X-Ray Diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopic (XPS).
32679332	6	24	theme	optimal	1138:1144	arg1	conditions					1146:1155	the optimal conditions	1134:1155	the optimal conditions for maximal absorption (~99%)	1134:1185	The results identified the best performing variants and the optimal conditions for maximal absorption (~99%).
32679332	0	25	theme	agrowaste-derived	76:92	arg1	Nanobiocomposites					119:135	agrowaste-derived Nanocellulose Iron Oxide Nanobiocomposites	76:135	agrowaste-derived Nanocellulose Iron Oxide Nanobiocomposites	76:135	Modelling and optimization of factors influencing adsorptive performance of agrowaste-derived Nanocellulose Iron Oxide Nanobiocomposites during remediation of Arsenic contaminated groundwater.
32679332	4	26	theme	Arsenic	825:831	arg1	mechanism					812:820	probable adsorption mechanism	792:820	probable adsorption mechanism of Arsenic by NIONs	792:840	FTIR and XPS data together with adsorption kinetics provide insights into probable adsorption mechanism of Arsenic by NIONs.
32679332	7	27	theme	feed-forward	1237:1248	arg1	MLP					1273:1275	MLP	1273:1275	MLP	1273:1275	These results were validated using a three-layer feed-forward Multilayer Perceptron (MLP) based Artificial Neural Network (ANN) model.
32679332	7	27	theme	feed-forward	1237:1248	arg1	Perceptron					1261:1270	three-layer feed-forward Multilayer Perceptron	1225:1270	three-layer feed-forward Multilayer Perceptron (MLP)	1225:1276	These results were validated using a three-layer feed-forward Multilayer Perceptron (MLP) based Artificial Neural Network (ANN) model.
32679332	8	28	theme	specific	1434:1441	arg1	variants					1443:1450	specific variants	1434:1450	specific variants	1434:1450	Both RSM and ANN chemometric models were in close conformity for optimized conditions of highest adsorption by specific variants.
32679332	3	29	theme	Transmission	561:572	arg1	TEM					595:597	TEM	595:597	TEM	595:597	Synthesis of NIONs was confirmed by Transmission electron microscopy (TEM), X-Ray Diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopic (XPS).
32679332	3	29	theme	Transmission	561:572	arg1	microscopy					583:592	Transmission electron microscopy	561:592	Transmission electron microscopy (TEM)	561:598	Synthesis of NIONs was confirmed by Transmission electron microscopy (TEM), X-Ray Diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopic (XPS).
32679332	3	29	theme	Transmission	561:572	arg1	Fourier					626:632	Fourier	626:632	Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopic (XPS)	626:715	Synthesis of NIONs was confirmed by Transmission electron microscopy (TEM), X-Ray Diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopic (XPS).
32679332	3	29	theme	Transmission	561:572	arg1	Diffraction					607:617	X-Ray Diffraction	601:617	X-Ray Diffraction (XRD)	601:623	Synthesis of NIONs was confirmed by Transmission electron microscopy (TEM), X-Ray Diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopic (XPS).
32679332	5	30	theme	response	917:924	arg1	RSM					947:949	RSM	947:949	RSM	947:949	The experimental conditions for 10 different variants were modelled using response surface methodology (RSM) based on central composite design (CCD), considering the parameters; adsorbate dosage, adsorbent dosage, pH and contact time.
32679332	5	30	theme	response	917:924	arg1	methodology					934:944	response surface methodology	917:944	response surface methodology (RSM) based on central composite design (CCD)	917:990	The experimental conditions for 10 different variants were modelled using response surface methodology (RSM) based on central composite design (CCD), considering the parameters; adsorbate dosage, adsorbent dosage, pH and contact time.
32679332	1	31	theme	arsenic	347:353	arg1	contaminants					370:381	arsenic and associated contaminants	347:381	arsenic and associated contaminants present in groundwater samples	347:412	Nanocellulose Iron Oxide Nanobiocomposites (NIONs) were synthesized from rice husk and sugarcane bagasse derived nanocelluloses for adsorptive removal of arsenic and associated contaminants present in groundwater samples.
32679332	6	32	theme	performing	1110:1119	arg1	variants					1121:1128	the best performing variants	1101:1128	the best performing variants	1101:1128	The results identified the best performing variants and the optimal conditions for maximal absorption (~99%).
32679332	2	33	theme	promising	500:508	arg1	recyclability					510:522	promising recyclability	500:522	promising recyclability	500:522	These NIONSs were superparamagnetic, hence magnetically recoverable and demonstrated promising recyclability.
32679332	0	34	theme	factors	30:36	arg1	optimization					14:25	optimization	14:25	optimization	14:25	Modelling and optimization of factors influencing adsorptive performance of agrowaste-derived Nanocellulose Iron Oxide Nanobiocomposites during remediation of Arsenic contaminated groundwater.
32679332	0	34	theme	factors	30:36	arg1	Modelling					0:8	Modelling	0:8	Modelling	0:8	Modelling and optimization of factors influencing adsorptive performance of agrowaste-derived Nanocellulose Iron Oxide Nanobiocomposites during remediation of Arsenic contaminated groundwater.
32679332	1	35	theme	associated	359:368	arg1	contaminants					370:381	arsenic and associated contaminants	347:381	arsenic and associated contaminants present in groundwater samples	347:412	Nanocellulose Iron Oxide Nanobiocomposites (NIONs) were synthesized from rice husk and sugarcane bagasse derived nanocelluloses for adsorptive removal of arsenic and associated contaminants present in groundwater samples.
32679332	1	36	from	samples	406:412	arg1	present					383:389	present	383:389	present	383:389	Nanocellulose Iron Oxide Nanobiocomposites (NIONs) were synthesized from rice husk and sugarcane bagasse derived nanocelluloses for adsorptive removal of arsenic and associated contaminants present in groundwater samples.
32679332	1	37	theme	present	383:389	arg1	contaminants					370:381	arsenic and associated contaminants	347:381	arsenic and associated contaminants present in groundwater samples	347:412	Nanocellulose Iron Oxide Nanobiocomposites (NIONs) were synthesized from rice husk and sugarcane bagasse derived nanocelluloses for adsorptive removal of arsenic and associated contaminants present in groundwater samples.
32679332	0	38	link	agrowaste-derived	76:92	arg1	Nanobiocomposites					119:135	agrowaste-derived Nanocellulose Iron Oxide Nanobiocomposites	76:135	agrowaste-derived Nanocellulose Iron Oxide Nanobiocomposites	76:135	Modelling and optimization of factors influencing adsorptive performance of agrowaste-derived Nanocellulose Iron Oxide Nanobiocomposites during remediation of Arsenic contaminated groundwater.
32679332	1	39	theme	husk	271:274	arg1	nanocelluloses					306:319	rice husk and sugarcane bagasse derived nanocelluloses	266:319	rice husk and sugarcane bagasse derived nanocelluloses	266:319	Nanocellulose Iron Oxide Nanobiocomposites (NIONs) were synthesized from rice husk and sugarcane bagasse derived nanocelluloses for adsorptive removal of arsenic and associated contaminants present in groundwater samples.
32679332	1	40	theme	Nanocellulose	193:205	arg1	NIONs					237:241	NIONs	237:241	NIONs	237:241	Nanocellulose Iron Oxide Nanobiocomposites (NIONs) were synthesized from rice husk and sugarcane bagasse derived nanocelluloses for adsorptive removal of arsenic and associated contaminants present in groundwater samples.
32679332	1	40	theme	Nanocellulose	193:205	arg1	Nanobiocomposites					218:234	Nanocellulose Iron Oxide Nanobiocomposites	193:234	Nanocellulose Iron Oxide Nanobiocomposites (NIONs)	193:242	Nanocellulose Iron Oxide Nanobiocomposites (NIONs) were synthesized from rice husk and sugarcane bagasse derived nanocelluloses for adsorptive removal of arsenic and associated contaminants present in groundwater samples.
32679332	10	41	theme	commercial	1636:1645	arg1	potential					1647:1655	promising commercial potential	1626:1655	promising commercial potential for water remediation applications due to their high adsorptive performance, magnetic recoverability and recyclability	1626:1774	NIONs show promising commercial potential for water remediation applications due to their high adsorptive performance, magnetic recoverability and recyclability.
32679332	3	42	dep	transform	634:642	arg1	infrared					644:651	infrared	644:651	transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopic (XPS)	634:715	Synthesis of NIONs was confirmed by Transmission electron microscopy (TEM), X-Ray Diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopic (XPS).
32679332	1	43	theme	sugarcane	280:288	arg1	bagasse					290:296	sugarcane bagasse	280:296	sugarcane bagasse	280:296	Nanocellulose Iron Oxide Nanobiocomposites (NIONs) were synthesized from rice husk and sugarcane bagasse derived nanocelluloses for adsorptive removal of arsenic and associated contaminants present in groundwater samples.
32679332	9	44	used	used	1486:1489	arg2	conditions					1470:1479	The standardized conditions	1453:1479	The standardized conditions	1453:1479	The standardized conditions were used to expand the study to field-based arsenic contaminated groundwater samples and their performance to commercial adsorbents.
32679332	5	45	theme	experimental	847:858	arg1	conditions					860:869	The experimental conditions	843:869	The experimental conditions for 10 different variants	843:895	The experimental conditions for 10 different variants were modelled using response surface methodology (RSM) based on central composite design (CCD), considering the parameters; adsorbate dosage, adsorbent dosage, pH and contact time.
32679332	1	46	from	present	383:389	arg1	samples					406:412	groundwater samples	394:412	groundwater samples	394:412	Nanocellulose Iron Oxide Nanobiocomposites (NIONs) were synthesized from rice husk and sugarcane bagasse derived nanocelluloses for adsorptive removal of arsenic and associated contaminants present in groundwater samples.
32679332	1	47	theme	derived	298:304	arg1	nanocelluloses					306:319	rice husk and sugarcane bagasse derived nanocelluloses	266:319	rice husk and sugarcane bagasse derived nanocelluloses	266:319	Nanocellulose Iron Oxide Nanobiocomposites (NIONs) were synthesized from rice husk and sugarcane bagasse derived nanocelluloses for adsorptive removal of arsenic and associated contaminants present in groundwater samples.
32679332	10	48	theme	remediation	1667:1677	arg1	applications					1679:1690	water remediation applications	1661:1690	water remediation applications due to their high adsorptive performance, magnetic recoverability and recyclability	1661:1774	NIONs show promising commercial potential for water remediation applications due to their high adsorptive performance, magnetic recoverability and recyclability.
32679332	0	49	theme	adsorptive	50:59	arg1	performance					61:71	adsorptive performance	50:71	adsorptive performance of agrowaste-derived Nanocellulose Iron Oxide Nanobiocomposites	50:135	Modelling and optimization of factors influencing adsorptive performance of agrowaste-derived Nanocellulose Iron Oxide Nanobiocomposites during remediation of Arsenic contaminated groundwater.
32679332	4	50	theme	XPS	727:729	arg1	data					731:734	FTIR and XPS data	718:734	data	731:734	FTIR and XPS data together with adsorption kinetics provide insights into probable adsorption mechanism of Arsenic by NIONs.
32679332	7	51	theme	based	1278:1282	arg1	model					1316:1320	a three-layer feed-forward Multilayer Perceptron (MLP) based Artificial Neural Network (ANN) model	1223:1320	a three-layer feed-forward Multilayer Perceptron (MLP) based Artificial Neural Network (ANN) model	1223:1320	These results were validated using a three-layer feed-forward Multilayer Perceptron (MLP) based Artificial Neural Network (ANN) model.
32679332	10	52	theme	due	1692:1694	arg1	applications					1679:1690	water remediation applications	1661:1690	water remediation applications due to their high adsorptive performance, magnetic recoverability and recyclability	1661:1774	NIONs show promising commercial potential for water remediation applications due to their high adsorptive performance, magnetic recoverability and recyclability.
32679332	0	53	theme	Arsenic	159:165	arg1	remediation					144:154	remediation	144:154	remediation of Arsenic	144:165	Modelling and optimization of factors influencing adsorptive performance of agrowaste-derived Nanocellulose Iron Oxide Nanobiocomposites during remediation of Arsenic contaminated groundwater.
32679332	9	54	theme	field-based	1514:1524	arg1	samples					1559:1565	field-based arsenic contaminated groundwater samples	1514:1565	field-based arsenic contaminated groundwater samples	1514:1565	The standardized conditions were used to expand the study to field-based arsenic contaminated groundwater samples and their performance to commercial adsorbents.
32679332	9	55	theme	contaminated	1534:1545	arg1	samples					1559:1565	field-based arsenic contaminated groundwater samples	1514:1565	field-based arsenic contaminated groundwater samples	1514:1565	The standardized conditions were used to expand the study to field-based arsenic contaminated groundwater samples and their performance to commercial adsorbents.
32679332	4	56	theme	FTIR	718:721	arg1	data					731:734	FTIR and XPS data	718:734	data	731:734	FTIR and XPS data together with adsorption kinetics provide insights into probable adsorption mechanism of Arsenic by NIONs.
32679332	10	57	theme	adsorptive	1710:1719	arg1	performance					1721:1731	their high adsorptive performance	1699:1731	their high adsorptive performance	1699:1731	NIONs show promising commercial potential for water remediation applications due to their high adsorptive performance, magnetic recoverability and recyclability.
32679332	4	58	theme	adsorption	801:810	arg1	mechanism					812:820	probable adsorption mechanism	792:820	probable adsorption mechanism of Arsenic by NIONs	792:840	FTIR and XPS data together with adsorption kinetics provide insights into probable adsorption mechanism of Arsenic by NIONs.
32679332	7	59	theme	Artificial	1284:1293	arg1	model					1316:1320	a three-layer feed-forward Multilayer Perceptron (MLP) based Artificial Neural Network (ANN) model	1223:1320	a three-layer feed-forward Multilayer Perceptron (MLP) based Artificial Neural Network (ANN) model	1223:1320	These results were validated using a three-layer feed-forward Multilayer Perceptron (MLP) based Artificial Neural Network (ANN) model.
32679332	3	60	theme	photoelectron	683:695	arg1	spectroscopic					697:709	X-ray photoelectron spectroscopic	677:709	X-ray photoelectron spectroscopic (XPS)	677:715	Synthesis of NIONs was confirmed by Transmission electron microscopy (TEM), X-Ray Diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopic (XPS).
32679332	3	60	theme	photoelectron	683:695	arg1	XPS					712:714	XPS	712:714	XPS	712:714	Synthesis of NIONs was confirmed by Transmission electron microscopy (TEM), X-Ray Diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopic (XPS).
32679332	5	61	theme	surface	926:932	arg1	RSM					947:949	RSM	947:949	RSM	947:949	The experimental conditions for 10 different variants were modelled using response surface methodology (RSM) based on central composite design (CCD), considering the parameters; adsorbate dosage, adsorbent dosage, pH and contact time.
32679332	5	61	theme	surface	926:932	arg1	methodology					934:944	response surface methodology	917:944	response surface methodology (RSM) based on central composite design (CCD)	917:990	The experimental conditions for 10 different variants were modelled using response surface methodology (RSM) based on central composite design (CCD), considering the parameters; adsorbate dosage, adsorbent dosage, pH and contact time.
32679332	5	62	theme	composite	969:977	arg1	CCD					987:989	CCD	987:989	CCD	987:989	The experimental conditions for 10 different variants were modelled using response surface methodology (RSM) based on central composite design (CCD), considering the parameters; adsorbate dosage, adsorbent dosage, pH and contact time.
32679332	5	62	theme	composite	969:977	arg1	design					979:984	central composite design	961:984	central composite design (CCD)	961:990	The experimental conditions for 10 different variants were modelled using response surface methodology (RSM) based on central composite design (CCD), considering the parameters; adsorbate dosage, adsorbent dosage, pH and contact time.
32679332	4	63	theme	adsorption	750:759	arg1	kinetics					761:768	adsorption kinetics	750:768	adsorption kinetics	750:768	FTIR and XPS data together with adsorption kinetics provide insights into probable adsorption mechanism of Arsenic by NIONs.
32679332	0	64	theme	Iron	108:111	arg1	Nanobiocomposites					119:135	agrowaste-derived Nanocellulose Iron Oxide Nanobiocomposites	76:135	agrowaste-derived Nanocellulose Iron Oxide Nanobiocomposites	76:135	Modelling and optimization of factors influencing adsorptive performance of agrowaste-derived Nanocellulose Iron Oxide Nanobiocomposites during remediation of Arsenic contaminated groundwater.
32679332	1	65	theme	Iron	207:210	arg1	NIONs					237:241	NIONs	237:241	NIONs	237:241	Nanocellulose Iron Oxide Nanobiocomposites (NIONs) were synthesized from rice husk and sugarcane bagasse derived nanocelluloses for adsorptive removal of arsenic and associated contaminants present in groundwater samples.
32679332	1	65	theme	Iron	207:210	arg1	Nanobiocomposites					218:234	Nanocellulose Iron Oxide Nanobiocomposites	193:234	Nanocellulose Iron Oxide Nanobiocomposites (NIONs)	193:242	Nanocellulose Iron Oxide Nanobiocomposites (NIONs) were synthesized from rice husk and sugarcane bagasse derived nanocelluloses for adsorptive removal of arsenic and associated contaminants present in groundwater samples.
32679332	8	66	theme	close	1367:1371	arg1	conformity					1373:1382	close conformity	1367:1382	close conformity for optimized conditions of highest adsorption by specific variants	1367:1450	Both RSM and ANN chemometric models were in close conformity for optimized conditions of highest adsorption by specific variants.
32679332	0	67	theme	Nanobiocomposites	119:135	arg1	performance					61:71	adsorptive performance	50:71	adsorptive performance of agrowaste-derived Nanocellulose Iron Oxide Nanobiocomposites	50:135	Modelling and optimization of factors influencing adsorptive performance of agrowaste-derived Nanocellulose Iron Oxide Nanobiocomposites during remediation of Arsenic contaminated groundwater.
32679332	5	68	theme	adsorbent	1039:1047	arg1	parameters					1009:1018	the parameters	1005:1018	the parameters; adsorbate dosage, adsorbent dosage, pH and contact time	1005:1075	The experimental conditions for 10 different variants were modelled using response surface methodology (RSM) based on central composite design (CCD), considering the parameters; adsorbate dosage, adsorbent dosage, pH and contact time.
32679332	5	68	theme	adsorbent	1039:1047	arg1	dosage					1049:1054	adsorbent dosage	1039:1054	adsorbent dosage	1039:1054	The experimental conditions for 10 different variants were modelled using response surface methodology (RSM) based on central composite design (CCD), considering the parameters; adsorbate dosage, adsorbent dosage, pH and contact time.
32679332	7	69	theme	Multilayer	1250:1259	arg1	MLP					1273:1275	MLP	1273:1275	MLP	1273:1275	These results were validated using a three-layer feed-forward Multilayer Perceptron (MLP) based Artificial Neural Network (ANN) model.
32679332	7	69	theme	Multilayer	1250:1259	arg1	Perceptron					1261:1270	three-layer feed-forward Multilayer Perceptron	1225:1270	three-layer feed-forward Multilayer Perceptron (MLP)	1225:1276	These results were validated using a three-layer feed-forward Multilayer Perceptron (MLP) based Artificial Neural Network (ANN) model.
32679332	2	70	dep	superparamagnetic	433:449	arg1	recoverable					471:481	recoverable	471:481	recoverable	471:481	These NIONSs were superparamagnetic, hence magnetically recoverable and demonstrated promising recyclability.
32679332	7	71	theme	three-layer	1225:1235	arg1	MLP					1273:1275	MLP	1273:1275	MLP	1273:1275	These results were validated using a three-layer feed-forward Multilayer Perceptron (MLP) based Artificial Neural Network (ANN) model.
32679332	7	71	theme	three-layer	1225:1235	arg1	Perceptron					1261:1270	three-layer feed-forward Multilayer Perceptron	1225:1270	three-layer feed-forward Multilayer Perceptron (MLP)	1225:1276	These results were validated using a three-layer feed-forward Multilayer Perceptron (MLP) based Artificial Neural Network (ANN) model.
32679332	8	72	theme	highest	1412:1418	arg1	adsorption					1420:1429	highest adsorption	1412:1429	highest adsorption by specific variants	1412:1450	Both RSM and ANN chemometric models were in close conformity for optimized conditions of highest adsorption by specific variants.
32679332	10	73	theme	magnetic	1734:1741	arg1	recoverability					1743:1756	magnetic recoverability	1734:1756	magnetic recoverability	1734:1756	NIONs show promising commercial potential for water remediation applications due to their high adsorptive performance, magnetic recoverability and recyclability.
32679332	5	74	theme	adsorbate	1021:1029	arg1	parameters					1009:1018	the parameters	1005:1018	the parameters; adsorbate dosage, adsorbent dosage, pH and contact time	1005:1075	The experimental conditions for 10 different variants were modelled using response surface methodology (RSM) based on central composite design (CCD), considering the parameters; adsorbate dosage, adsorbent dosage, pH and contact time.
32679332	5	74	theme	adsorbate	1021:1029	arg1	dosage					1031:1036	adsorbate dosage	1021:1036	adsorbate dosage	1021:1036	The experimental conditions for 10 different variants were modelled using response surface methodology (RSM) based on central composite design (CCD), considering the parameters; adsorbate dosage, adsorbent dosage, pH and contact time.
32679332	8	75	theme	adsorption	1420:1429	arg1	conditions					1398:1407	optimized conditions	1388:1407	optimized conditions of highest adsorption by specific variants	1388:1450	Both RSM and ANN chemometric models were in close conformity for optimized conditions of highest adsorption by specific variants.
32679332	7	76	dep	based	1278:1282	arg1	MLP					1273:1275	MLP	1273:1275	MLP	1273:1275	These results were validated using a three-layer feed-forward Multilayer Perceptron (MLP) based Artificial Neural Network (ANN) model.
32679332	7	76	dep	based	1278:1282	arg1	Perceptron					1261:1270	three-layer feed-forward Multilayer Perceptron	1225:1270	three-layer feed-forward Multilayer Perceptron (MLP)	1225:1276	These results were validated using a three-layer feed-forward Multilayer Perceptron (MLP) based Artificial Neural Network (ANN) model.
32679332	3	77	theme	electron	574:581	arg1	TEM					595:597	TEM	595:597	TEM	595:597	Synthesis of NIONs was confirmed by Transmission electron microscopy (TEM), X-Ray Diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopic (XPS).
32679332	3	77	theme	electron	574:581	arg1	microscopy					583:592	Transmission electron microscopy	561:592	Transmission electron microscopy (TEM)	561:598	Synthesis of NIONs was confirmed by Transmission electron microscopy (TEM), X-Ray Diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopic (XPS).
32679332	3	77	theme	electron	574:581	arg1	Fourier					626:632	Fourier	626:632	Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopic (XPS)	626:715	Synthesis of NIONs was confirmed by Transmission electron microscopy (TEM), X-Ray Diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopic (XPS).
32679332	3	77	theme	electron	574:581	arg1	Diffraction					607:617	X-Ray Diffraction	601:617	X-Ray Diffraction (XRD)	601:623	Synthesis of NIONs was confirmed by Transmission electron microscopy (TEM), X-Ray Diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopic (XPS).
32679332	5	78	theme	different	878:886	arg1	variants					888:895	10 different variants	875:895	10 different variants	875:895	The experimental conditions for 10 different variants were modelled using response surface methodology (RSM) based on central composite design (CCD), considering the parameters; adsorbate dosage, adsorbent dosage, pH and contact time.
32679332	1	79	theme	contaminants	370:381	arg1	removal					336:342	adsorptive removal	325:342	adsorptive removal of arsenic and associated contaminants present in groundwater samples	325:412	Nanocellulose Iron Oxide Nanobiocomposites (NIONs) were synthesized from rice husk and sugarcane bagasse derived nanocelluloses for adsorptive removal of arsenic and associated contaminants present in groundwater samples.
32679332	8	80	dep	models	1352:1357	arg1	chemometric					1340:1350	chemometric	1340:1350	chemometric	1340:1350	Both RSM and ANN chemometric models were in close conformity for optimized conditions of highest adsorption by specific variants.
32679332	8	80	dep	models	1352:1357	arg1	Both					1323:1326	Both	1323:1326	Both	1323:1326	Both RSM and ANN chemometric models were in close conformity for optimized conditions of highest adsorption by specific variants.
32679332	1	81	theme	rice	266:269	arg1	husk					271:274	rice husk	266:274	rice husk	266:274	Nanocellulose Iron Oxide Nanobiocomposites (NIONs) were synthesized from rice husk and sugarcane bagasse derived nanocelluloses for adsorptive removal of arsenic and associated contaminants present in groundwater samples.
32679332	5	82	theme	contact	1064:1070	arg1	parameters					1009:1018	the parameters	1005:1018	the parameters; adsorbate dosage, adsorbent dosage, pH and contact time	1005:1075	The experimental conditions for 10 different variants were modelled using response surface methodology (RSM) based on central composite design (CCD), considering the parameters; adsorbate dosage, adsorbent dosage, pH and contact time.
32679332	5	82	theme	contact	1064:1070	arg1	time					1072:1075	contact time	1064:1075	contact time	1064:1075	The experimental conditions for 10 different variants were modelled using response surface methodology (RSM) based on central composite design (CCD), considering the parameters; adsorbate dosage, adsorbent dosage, pH and contact time.
32679332	7	83	theme	ANN	1311:1313	arg1	model					1316:1320	a three-layer feed-forward Multilayer Perceptron (MLP) based Artificial Neural Network (ANN) model	1223:1320	a three-layer feed-forward Multilayer Perceptron (MLP) based Artificial Neural Network (ANN) model	1223:1320	These results were validated using a three-layer feed-forward Multilayer Perceptron (MLP) based Artificial Neural Network (ANN) model.
32679332	10	84	theme	promising	1626:1634	arg1	potential					1647:1655	promising commercial potential	1626:1655	promising commercial potential for water remediation applications due to their high adsorptive performance, magnetic recoverability and recyclability	1626:1774	NIONs show promising commercial potential for water remediation applications due to their high adsorptive performance, magnetic recoverability and recyclability.
32679332	1	85	theme	bagasse	290:296	arg1	nanocelluloses					306:319	rice husk and sugarcane bagasse derived nanocelluloses	266:319	rice husk and sugarcane bagasse derived nanocelluloses	266:319	Nanocellulose Iron Oxide Nanobiocomposites (NIONs) were synthesized from rice husk and sugarcane bagasse derived nanocelluloses for adsorptive removal of arsenic and associated contaminants present in groundwater samples.
32679332	9	86	theme	standardized	1457:1468	arg1	conditions					1470:1479	The standardized conditions	1453:1479	The standardized conditions	1453:1479	The standardized conditions were used to expand the study to field-based arsenic contaminated groundwater samples and their performance to commercial adsorbents.
33082711	7	0	dep	number	904:909	arg1	the					900:902	the	900:902	the	900:902	Apigenin was unable to reduce the number or size of tumors when gut microbiota were depleted.
33082711	6	1	theme	Further	694:700	arg1	experiments					702:712	Further experiments	694:712	Further experiments in which gut microbiota were reduced	694:749	Further experiments in which gut microbiota were reduced and feces were transplanted provided further evidence of apigenin-modulated gut microbiota exerting antitumor effects.
33082711	4	2	theme	treatment	543:551	arg1	group					553:557	the apigenin treatment group	530:557	the apigenin treatment group	530:557	The size and quantity of tumors were reduced significantly in the apigenin treatment group.
33082711	0	3	theme	Gut	71:73	arg1	Microbiota					75:84	the Gut Microbiota	67:84	the Gut Microbiota	67:84	Inhibitory Effects of Apigenin on Tumor Carcinogenesis by Altering the Gut Microbiota.
33082711	0	4	from	Effects	11:17	arg1	Carcinogenesis					40:53	Tumor Carcinogenesis	34:53	Tumor Carcinogenesis	34:53	Inhibitory Effects of Apigenin on Tumor Carcinogenesis by Altering the Gut Microbiota.
33082711	6	5	theme	antitumor	851:859	arg1	effects					861:867	antitumor effects	851:867	antitumor effects	851:867	Further experiments in which gut microbiota were reduced and feces were transplanted provided further evidence of apigenin-modulated gut microbiota exerting antitumor effects.
33082711	4	6	theme	apigenin	534:541	arg1	group					553:557	the apigenin treatment group	530:557	the apigenin treatment group	530:557	The size and quantity of tumors were reduced significantly in the apigenin treatment group.
33082711	8	7	theme	feces	1042:1046	arg1	transplant					1028:1037	the transplant	1024:1037	the transplant of feces from mice treated with apigenin	1024:1078	Moreover, tumor inhibition effects were initiated following the transplant of feces from mice treated with apigenin.
33082711	5	8	theme	16S	566:568	arg1	sequencing					591:600	16S rRNA high-throughput sequencing	566:600	16S rRNA high-throughput sequencing of fecal samples	566:617	Using 16S rRNA high-throughput sequencing of fecal samples, the composition of gut microbiota was significantly affected by apigenin.
33082711	9	9	theme	microbiota	1156:1165	arg1	composition					1137:1147	the composition	1133:1147	the composition of gut microbiota	1133:1165	Our findings suggest that the effect of apigenin on the composition of gut microbiota can suppress tumors.
33082711	5	10	theme	rRNA	570:573	arg1	sequencing					591:600	16S rRNA high-throughput sequencing	566:600	16S rRNA high-throughput sequencing of fecal samples	566:617	Using 16S rRNA high-throughput sequencing of fecal samples, the composition of gut microbiota was significantly affected by apigenin.
33082711	5	11	theme	high-throughput	575:589	arg1	sequencing					591:600	16S rRNA high-throughput sequencing	566:600	16S rRNA high-throughput sequencing of fecal samples	566:617	Using 16S rRNA high-throughput sequencing of fecal samples, the composition of gut microbiota was significantly affected by apigenin.
33082711	9	12	from	effect	1111:1116	arg1	composition					1137:1147	the composition	1133:1147	the composition of gut microbiota	1133:1165	Our findings suggest that the effect of apigenin on the composition of gut microbiota can suppress tumors.
33082711	0	13	theme	Inhibitory	0:9	arg1	Effects					11:17	Inhibitory Effects	0:17	Inhibitory Effects of Apigenin on Tumor Carcinogenesis	0:53	Inhibitory Effects of Apigenin on Tumor Carcinogenesis by Altering the Gut Microbiota.
33082711	6	14	dep	transplanted	766:777	arg1	provided					779:786	provided	779:786	were transplanted provided further evidence of apigenin-modulated gut microbiota exerting antitumor effects	761:867	Further experiments in which gut microbiota were reduced and feces were transplanted provided further evidence of apigenin-modulated gut microbiota exerting antitumor effects.
33082711	3	15	theme	cancer	344:349	arg1	model					357:361	an azoxymethane (AOM)/dextran sodium sulfate- (DSS-) induced colon cancer mouse model	277:361	an azoxymethane (AOM)/dextran sodium sulfate- (DSS-) induced colon cancer mouse model to investigate apigenin's potential mechanism of action exerted through its effects upon gut microbiota	277:465	This study uses an azoxymethane (AOM)/dextran sodium sulfate- (DSS-) induced colon cancer mouse model to investigate apigenin's potential mechanism of action exerted through its effects upon gut microbiota.
33082711	5	16	theme	fecal	605:609	arg1	samples					611:617	fecal samples	605:617	fecal samples	605:617	Using 16S rRNA high-throughput sequencing of fecal samples, the composition of gut microbiota was significantly affected by apigenin.
33082711	7	17	theme	gut	934:936	arg1	microbiota					938:947	gut microbiota	934:947	gut microbiota	934:947	Apigenin was unable to reduce the number or size of tumors when gut microbiota were depleted.
33082711	3	18	theme	mouse	351:355	arg1	model					357:361	an azoxymethane (AOM)/dextran sodium sulfate- (DSS-) induced colon cancer mouse model	277:361	an azoxymethane (AOM)/dextran sodium sulfate- (DSS-) induced colon cancer mouse model to investigate apigenin's potential mechanism of action exerted through its effects upon gut microbiota	277:465	This study uses an azoxymethane (AOM)/dextran sodium sulfate- (DSS-) induced colon cancer mouse model to investigate apigenin's potential mechanism of action exerted through its effects upon gut microbiota.
33082711	0	19	theme	Apigenin	22:29	arg1	Effects					11:17	Inhibitory Effects	0:17	Inhibitory Effects of Apigenin on Tumor Carcinogenesis	0:53	Inhibitory Effects of Apigenin on Tumor Carcinogenesis by Altering the Gut Microbiota.
33082711	3	20	theme	potential	389:397	arg1	mechanism					399:407	apigenin's potential mechanism	378:407	apigenin's potential mechanism of action exerted through its effects upon gut microbiota	378:465	This study uses an azoxymethane (AOM)/dextran sodium sulfate- (DSS-) induced colon cancer mouse model to investigate apigenin's potential mechanism of action exerted through its effects upon gut microbiota.
33082711	3	21	theme	induced	330:336	arg1	model					357:361	an azoxymethane (AOM)/dextran sodium sulfate- (DSS-) induced colon cancer mouse model	277:361	an azoxymethane (AOM)/dextran sodium sulfate- (DSS-) induced colon cancer mouse model to investigate apigenin's potential mechanism of action exerted through its effects upon gut microbiota	277:465	This study uses an azoxymethane (AOM)/dextran sodium sulfate- (DSS-) induced colon cancer mouse model to investigate apigenin's potential mechanism of action exerted through its effects upon gut microbiota.
33082711	7	22	theme	tumors	922:927	arg1	number					904:909	number	904:909	number	904:909	Apigenin was unable to reduce the number or size of tumors when gut microbiota were depleted.
33082711	7	22	theme	tumors	922:927	arg1	size					914:917	size	914:917	size	914:917	Apigenin was unable to reduce the number or size of tumors when gut microbiota were depleted.
33082711	1	23	theme	flavonoid	91:99	arg1	common					113:118	common	113:118	common	113:118	The flavonoid apigenin is common to many plants.
33082711	1	23	theme	flavonoid	91:99	arg1	apigenin					101:108	The flavonoid apigenin	87:108	The flavonoid apigenin	87:108	The flavonoid apigenin is common to many plants.
33082711	3	24	theme	colon	338:342	arg1	model					357:361	an azoxymethane (AOM)/dextran sodium sulfate- (DSS-) induced colon cancer mouse model	277:361	an azoxymethane (AOM)/dextran sodium sulfate- (DSS-) induced colon cancer mouse model to investigate apigenin's potential mechanism of action exerted through its effects upon gut microbiota	277:465	This study uses an azoxymethane (AOM)/dextran sodium sulfate- (DSS-) induced colon cancer mouse model to investigate apigenin's potential mechanism of action exerted through its effects upon gut microbiota.
33082711	6	25	theme	further	788:794	arg1	evidence					796:803	further evidence	788:803	further evidence of apigenin-modulated gut microbiota exerting antitumor effects	788:867	Further experiments in which gut microbiota were reduced and feces were transplanted provided further evidence of apigenin-modulated gut microbiota exerting antitumor effects.
33082711	0	26	theme	Tumor	34:38	arg1	Carcinogenesis					40:53	Tumor Carcinogenesis	34:53	Tumor Carcinogenesis	34:53	Inhibitory Effects of Apigenin on Tumor Carcinogenesis by Altering the Gut Microbiota.
33082711	3	27	theme	action	412:417	arg1	mechanism					399:407	apigenin's potential mechanism	378:407	apigenin's potential mechanism of action exerted through its effects upon gut microbiota	378:465	This study uses an azoxymethane (AOM)/dextran sodium sulfate- (DSS-) induced colon cancer mouse model to investigate apigenin's potential mechanism of action exerted through its effects upon gut microbiota.
33082711	8	28	theme	inhibition	980:989	arg1	effects					991:997	tumor inhibition effects	974:997	tumor inhibition effects	974:997	Moreover, tumor inhibition effects were initiated following the transplant of feces from mice treated with apigenin.
33082711	2	29	theme	tumor	221:225	arg1	suppression					227:237	tumor suppression	221:237	tumor suppression	221:237	Although the responsible mechanisms have yet to be elucidated, apigenin demonstrates tumor suppression in vitro and in vivo.
33082711	4	30	dep	size	472:475	arg1	The					468:470	The	468:470	The	468:470	The size and quantity of tumors were reduced significantly in the apigenin treatment group.
33082711	5	31	theme	gut	639:641	arg1	microbiota					643:652	gut microbiota	639:652	gut microbiota	639:652	Using 16S rRNA high-throughput sequencing of fecal samples, the composition of gut microbiota was significantly affected by apigenin.
33082711	2	32	theme	responsible	149:159	arg1	mechanisms					161:170	the responsible mechanisms	145:170	the responsible mechanisms	145:170	Although the responsible mechanisms have yet to be elucidated, apigenin demonstrates tumor suppression in vitro and in vivo.
33082711	9	33	theme	apigenin	1121:1128	arg1	effect					1111:1116	the effect	1107:1116	the effect of apigenin on the composition of gut microbiota	1107:1165	Our findings suggest that the effect of apigenin on the composition of gut microbiota can suppress tumors.
33082711	3	34	theme	gut	452:454	arg1	microbiota					456:465	gut microbiota	452:465	gut microbiota	452:465	This study uses an azoxymethane (AOM)/dextran sodium sulfate- (DSS-) induced colon cancer mouse model to investigate apigenin's potential mechanism of action exerted through its effects upon gut microbiota.
33082711	8	35	from	mice	1053:1056	arg1	transplant					1028:1037	the transplant	1024:1037	the transplant of feces from mice treated with apigenin	1024:1078	Moreover, tumor inhibition effects were initiated following the transplant of feces from mice treated with apigenin.
33082711	5	36	theme	microbiota	643:652	arg1	composition					624:634	the composition	620:634	the composition of gut microbiota	620:652	Using 16S rRNA high-throughput sequencing of fecal samples, the composition of gut microbiota was significantly affected by apigenin.
33082711	5	37	theme	samples	611:617	arg1	sequencing					591:600	16S rRNA high-throughput sequencing	566:600	16S rRNA high-throughput sequencing of fecal samples	566:617	Using 16S rRNA high-throughput sequencing of fecal samples, the composition of gut microbiota was significantly affected by apigenin.
33082711	4	38	theme	tumors	493:498	arg1	quantity					481:488	quantity	481:488	quantity	481:488	The size and quantity of tumors were reduced significantly in the apigenin treatment group.
33082711	4	38	theme	tumors	493:498	arg1	size					472:475	size	472:475	size	472:475	The size and quantity of tumors were reduced significantly in the apigenin treatment group.
33082711	6	39	theme	microbiota	831:840	arg1	evidence					796:803	further evidence	788:803	further evidence of apigenin-modulated gut microbiota exerting antitumor effects	788:867	Further experiments in which gut microbiota were reduced and feces were transplanted provided further evidence of apigenin-modulated gut microbiota exerting antitumor effects.
33082711	6	40	theme	gut	723:725	arg1	microbiota					727:736	gut microbiota	723:736	gut microbiota	723:736	Further experiments in which gut microbiota were reduced and feces were transplanted provided further evidence of apigenin-modulated gut microbiota exerting antitumor effects.
33082711	8	41	theme	tumor	974:978	arg1	effects					991:997	tumor inhibition effects	974:997	tumor inhibition effects	974:997	Moreover, tumor inhibition effects were initiated following the transplant of feces from mice treated with apigenin.
33082711	6	42	theme	gut	827:829	arg1	microbiota					831:840	apigenin-modulated gut microbiota	808:840	apigenin-modulated gut microbiota exerting antitumor effects	808:867	Further experiments in which gut microbiota were reduced and feces were transplanted provided further evidence of apigenin-modulated gut microbiota exerting antitumor effects.
33082711	1	43	theme	many	123:126	arg1	plants					128:133	many plants	123:133	many plants	123:133	The flavonoid apigenin is common to many plants.
33082711	6	44	theme	apigenin-modulated	808:825	arg1	microbiota					831:840	apigenin-modulated gut microbiota	808:840	apigenin-modulated gut microbiota exerting antitumor effects	808:867	Further experiments in which gut microbiota were reduced and feces were transplanted provided further evidence of apigenin-modulated gut microbiota exerting antitumor effects.
33082711	9	45	theme	gut	1152:1154	arg1	microbiota					1156:1165	gut microbiota	1152:1165	gut microbiota	1152:1165	Our findings suggest that the effect of apigenin on the composition of gut microbiota can suppress tumors.
33818824	31	0	dep	Authors	5538:5544	arg1	2021					5529:5532	© 2021	5527:5532	© 2021	5527:5532	© 2021 The Authors.
33818824	9	1	theme	AOPs	1529:1532	arg1	lack					1501:1504	a lack	1499:1504	a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors)	1499:1795	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	15	2	theme	successful	2826:2835	arg1	Cohen					2846:2850	Cohen	2846:2850	Cohen	2846:2850	Because the procuticle mainly consists of chitin microfibrils embedded in a matrix of cuticular proteins supplemented by lipids and minerals in insects (Muthukrishnan et al. 2012) and crustaceans (Cribb et al. 2009; Nagasawa 2012), chitin is a determinant factor for the appropriate composition of the cuticle and successful molting (Cohen 2001).
33818824	15	2	theme	successful	2826:2835	arg1	molting					2837:2843	successful molting	2826:2843	successful molting (Cohen 2001)	2826:2856	Because the procuticle mainly consists of chitin microfibrils embedded in a matrix of cuticular proteins supplemented by lipids and minerals in insects (Muthukrishnan et al. 2012) and crustaceans (Cribb et al. 2009; Nagasawa 2012), chitin is a determinant factor for the appropriate composition of the cuticle and successful molting (Cohen 2001).
33818824	25	3	dep	molts	4556:4560	arg1	Arakawa					4563:4569	Arakawa	4563:4569	Arakawa	4563:4569	These effects are observed in arthropods following molting, which fail to survive subsequent molts (Arakawa et al. 2008; Chen et al. 2008) or animals being stuck in their exuviae (Wang et al. 2019) and ultimately dying as a result of insufficient food or oxygen intake (Camp et al. 2014; Song et al. 2017a).
33818824	5	4	dep	Ankley	984:989	arg1	2010					998:1001	2010	998:1001	Ankley et al. 2010	984:1001	The need for a more cost-efficient and mechanistic approach in risk assessment has been clearly evident and triggered the development of the adverse outcome pathway (AOP) framework (Ankley et al. 2010).
33818824	5	4	dep	Ankley	984:989	arg1	al.					994:996	Ankley et al.	984:996	Ankley et al. 2010	984:1001	The need for a more cost-efficient and mechanistic approach in risk assessment has been clearly evident and triggered the development of the adverse outcome pathway (AOP) framework (Ankley et al. 2010).
33818824	19	5	from	localized	3488:3496	arg1	membrane					3523:3530	the epithelial plasma membrane	3501:3530	the epithelial plasma membrane in insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006)	3501:3629	Cuticular chitin is polymerized from uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) by the transmembrane enzyme CHS-1, which is localized in the epithelial plasma membrane in insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006).
33818824	2	6	theme	process	302:308	arg1	process					270:276	The highly conserved chitin synthetic process	232:276	The highly conserved chitin synthetic process	232:276	The highly conserved chitin synthetic process and the absence of this process in vertebrates make it an exploitable target for pest management and veterinary medicines (Merzendorfer 2013; Junquera et al. 2019).
33818824	2	6	theme	process	302:308	arg1	absence					286:292	the absence	282:292	the absence of this process in vertebrates	282:323	The highly conserved chitin synthetic process and the absence of this process in vertebrates make it an exploitable target for pest management and veterinary medicines (Merzendorfer 2013; Junquera et al. 2019).
33818824	16	7	theme	Figure	2966:2971	arg1	Data					2960:2963	Supplemental Data	2947:2963	Supplemental Data	2947:2963	A detailed overview of the endocrine mechanisms regulating chitin synthesis is given in Supplemental Data, Figure S1.
33818824	16	7	theme	Figure	2966:2971	arg1	S1					2973:2974	Figure S1	2966:2974	Figure S1	2966:2974	A detailed overview of the endocrine mechanisms regulating chitin synthesis is given in Supplemental Data, Figure S1.
33818824	1	8	theme	Merzendorfer	212:223	arg1	cycles					204:209	their life cycles	193:209	their life cycles (Merzendorfer 2011)	193:229	Arthropods (including insects, crustaceans, and arachnids) rely on the synthesis of chitin to complete their life cycles (Merzendorfer 2011).
33818824	1	8	theme	Merzendorfer	212:223	arg1	2011					225:228	Merzendorfer 2011	212:228	Merzendorfer 2011	212:228	Arthropods (including insects, crustaceans, and arachnids) rely on the synthesis of chitin to complete their life cycles (Merzendorfer 2011).
33818824	9	9	theme	molting	1545:1551	arg1	mortality					1571:1579	molting defect-associated mortality	1545:1579	molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors)	1545:1795	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	7	10	theme	taxonomic	1337:1345	arg1	domains					1347:1353	wider chemical and taxonomic domains	1318:1353	wider chemical and taxonomic domains	1318:1353	The mechanistic understanding of the underlying toxicological processes leading to a regulation-relevant adverse outcome is necessary for the utilization of new approach methodologies (NAMs) and efficient coverage of wider chemical and taxonomic domains.
33818824	28	11	theme	small	5096:5100	arg1	number					5117:5122	the small but increasing number	5092:5122	the small but increasing number of invertebrate AOPs that have relevance to arthropods, the largest phylum within the animal kingdom (Bar-On et al. 2018)	5092:5244	It expands the small but increasing number of invertebrate AOPs that have relevance to arthropods, the largest phylum within the animal kingdom (Bar-On et al. 2018).
33818824	5	12	dep	framework	973:981	arg1	Ankley					984:989	Ankley	984:989	Ankley	984:989	The need for a more cost-efficient and mechanistic approach in risk assessment has been clearly evident and triggered the development of the adverse outcome pathway (AOP) framework (Ankley et al. 2010).
33818824	22	13	theme	inhibition	4132:4141	arg1	case					4107:4110	the case	4103:4110	the case of chitin synthesis inhibition	4103:4141	In the case of chitin synthesis inhibition, molting disruption can be referred to as "premature molting."
33818824	19	14	theme	Cuticular	3354:3362	arg1	chitin					3364:3369	Cuticular chitin	3354:3369	Cuticular chitin	3354:3369	Cuticular chitin is polymerized from uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) by the transmembrane enzyme CHS-1, which is localized in the epithelial plasma membrane in insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006).
33818824	22	15	theme	molting	4144:4150	arg1	disruption					4152:4161	molting disruption	4144:4161	molting disruption	4144:4161	In the case of chitin synthesis inhibition, molting disruption can be referred to as "premature molting."
33818824	22	15	theme	molting	4144:4150	arg1	molting					4196:4202	"premature molting	4185:4202	"premature molting	4185:4202	In the case of chitin synthesis inhibition, molting disruption can be referred to as "premature molting."
33818824	4	16	theme	nontarget	780:788	arg1	arthropods					790:799	nontarget arthropods	780:799	nontarget arthropods	780:799	Hence, it is important to properly identify, prioritize, and regulate relevant chemicals posing potential hazards to nontarget arthropods.
33818824	7	17	theme	chemical	1324:1331	arg1	domains					1347:1353	wider chemical and taxonomic domains	1318:1353	wider chemical and taxonomic domains	1318:1353	The mechanistic understanding of the underlying toxicological processes leading to a regulation-relevant adverse outcome is necessary for the utilization of new approach methodologies (NAMs) and efficient coverage of wider chemical and taxonomic domains.
33818824	25	18	dep	Wang	4643:4646	arg1	al.					4651:4653	Wang et al.	4643:4653	Wang et al. 2019	4643:4658	These effects are observed in arthropods following molting, which fail to survive subsequent molts (Arakawa et al. 2008; Chen et al. 2008) or animals being stuck in their exuviae (Wang et al. 2019) and ultimately dying as a result of insufficient food or oxygen intake (Camp et al. 2014; Song et al. 2017a).
33818824	25	18	dep	Wang	4643:4646	arg1	2019					4655:4658	2019	4655:4658	Wang et al. 2019	4643:4658	These effects are observed in arthropods following molting, which fail to survive subsequent molts (Arakawa et al. 2008; Chen et al. 2008) or animals being stuck in their exuviae (Wang et al. 2019) and ultimately dying as a result of insufficient food or oxygen intake (Camp et al. 2014; Song et al. 2017a).
33818824	27	19	theme	molting-associated	5005:5022	arg1	mortality					5024:5032	molting-associated mortality	5005:5032	molting-associated mortality	5005:5032	The present AOP describes molting-associated mortality through direct inhibition of the enzyme CHS-1.
33818824	23	20	theme	decreased	4248:4256	arg1	synthesis					4265:4273	decreased chitin synthesis	4248:4273	decreased chitin synthesis	4248:4273	If ecdysis cannot be completed because of decreased chitin synthesis, the organism may not successfully molt.
33818824	11	21	theme	new	1910:1912	arg1	exoskeleton					1914:1924	a new exoskeleton	1908:1924	a new exoskeleton	1908:1924	This process is comprised of the synthesis of a new exoskeleton, followed by the exuviation of the old exoskeleton (Reynolds 1987).
33818824	6	22	theme	key	1061:1063	arg1	KEs					1073:1075	KEs	1073:1075	KEs	1073:1075	An AOP links a molecular initiating event (MIE) through key events (KEs) to an adverse outcome.
33818824	6	22	theme	key	1061:1063	arg1	events					1065:1070	key events	1061:1070	key events (KEs)	1061:1076	An AOP links a molecular initiating event (MIE) through key events (KEs) to an adverse outcome.
33818824	26	23	theme	behavioral	4904:4913	arg1	level					4915:4919	a behavioral level	4902:4919	a behavioral level	4902:4919	The term "premature molting" is used to differentiate from the term "incomplete ecdysis," which describes inhibition of ecdysis on a behavioral level, namely through reduction of the EMP (Song et al. 2017a).
33818824	26	24	theme	premature	4781:4789	arg1	"					4798:4798	The term "premature molting"	4771:4798	The term "premature molting"	4771:4798	The term "premature molting" is used to differentiate from the term "incomplete ecdysis," which describes inhibition of ecdysis on a behavioral level, namely through reduction of the EMP (Song et al. 2017a).
33818824	15	25	theme	proteins	2608:2615	arg1	matrix					2588:2593	a matrix	2586:2593	a matrix of cuticular proteins supplemented by lipids and minerals in insects (Muthukrishnan et al. 2012) and crustaceans (Cribb et al. 2009; Nagasawa 2012)	2586:2741	Because the procuticle mainly consists of chitin microfibrils embedded in a matrix of cuticular proteins supplemented by lipids and minerals in insects (Muthukrishnan et al. 2012) and crustaceans (Cribb et al. 2009; Nagasawa 2012), chitin is a determinant factor for the appropriate composition of the cuticle and successful molting (Cohen 2001).
33818824	25	26	theme	subsequent	4545:4554	arg1	molts					4556:4560	subsequent molts	4545:4560	subsequent molts (Arakawa et al. 2008; Chen et al. 2008)	4545:4600	These effects are observed in arthropods following molting, which fail to survive subsequent molts (Arakawa et al. 2008; Chen et al. 2008) or animals being stuck in their exuviae (Wang et al. 2019) and ultimately dying as a result of insufficient food or oxygen intake (Camp et al. 2014; Song et al. 2017a).
33818824	16	27	theme	mechanisms	2896:2905	arg1	overview					2870:2877	A detailed overview	2859:2877	A detailed overview of the endocrine mechanisms regulating chitin synthesis	2859:2933	A detailed overview of the endocrine mechanisms regulating chitin synthesis is given in Supplemental Data, Figure S1.
33818824	11	28	dep	comprised	1878:1886	arg1	Reynolds					1978:1985	Reynolds	1978:1985	Reynolds	1978:1985	This process is comprised of the synthesis of a new exoskeleton, followed by the exuviation of the old exoskeleton (Reynolds 1987).
33818824	11	28	dep	comprised	1878:1886	arg1	followed					1927:1934	followed	1927:1934	followed by the exuviation of the old exoskeleton	1927:1975	This process is comprised of the synthesis of a new exoskeleton, followed by the exuviation of the old exoskeleton (Reynolds 1987).
33818824	21	29	theme	lethal	3999:4004	arg1	Arakawa					4026:4032	Arakawa	4026:4032	Arakawa	4026:4032	Disruption of either chitin synthesis or the upstream endocrine pathways can lead to lethal molting disruption (Arakawa et al. 2008; Merzendorfer et al. 2012; Song et al. 2017a, 2017b).
33818824	21	29	theme	lethal	3999:4004	arg1	disruption					4014:4023	lethal molting disruption	3999:4023	lethal molting disruption (Arakawa et al. 2008; Merzendorfer et al. 2012; Song et al. 2017a, 2017b)	3999:4097	Disruption of either chitin synthesis or the upstream endocrine pathways can lead to lethal molting disruption (Arakawa et al. 2008; Merzendorfer et al. 2012; Song et al. 2017a, 2017b).
33818824	11	30	theme	old	1961:1963	arg1	exoskeleton					1965:1975	the old exoskeleton	1957:1975	the old exoskeleton	1957:1975	This process is comprised of the synthesis of a new exoskeleton, followed by the exuviation of the old exoskeleton (Reynolds 1987).
33818824	19	31	theme	uridine	3391:3397	arg1	diphosphate-N-acetylglucosamine					3399:3429	uridine diphosphate-N-acetylglucosamine	3391:3429	uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) by the transmembrane enzyme CHS-1, which is localized in the epithelial plasma membrane in insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006)	3391:3629	Cuticular chitin is polymerized from uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) by the transmembrane enzyme CHS-1, which is localized in the epithelial plasma membrane in insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006).
33818824	19	31	theme	uridine	3391:3397	arg1	UDP-GlcNAc					3432:3441	UDP-GlcNAc	3432:3441	UDP-GlcNAc	3432:3441	Cuticular chitin is polymerized from uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) by the transmembrane enzyme CHS-1, which is localized in the epithelial plasma membrane in insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006).
33818824	7	32	theme	efficient	1296:1304	arg1	coverage					1306:1313	efficient coverage	1296:1313	efficient coverage of wider chemical and taxonomic domains	1296:1353	The mechanistic understanding of the underlying toxicological processes leading to a regulation-relevant adverse outcome is necessary for the utilization of new approach methodologies (NAMs) and efficient coverage of wider chemical and taxonomic domains.
33818824	13	33	dep	responsible	2222:2232	arg1	responsible					2222:2232	responsible	2222:2232	responsible	2222:2232	A single layer of epithelial cells is responsible for the synthesis and secretion of both cuticular layers (Neville 1975).
33818824	13	33	dep	responsible	2222:2232	arg1	Neville					2292:2298	Neville	2292:2298	Neville	2292:2298	A single layer of epithelial cells is responsible for the synthesis and secretion of both cuticular layers (Neville 1975).
33818824	13	33	dep	responsible	2222:2232	arg1	layer					2193:2197	A single layer	2184:2197	A single layer of epithelial cells	2184:2217	A single layer of epithelial cells is responsible for the synthesis and secretion of both cuticular layers (Neville 1975).
33818824	29	34	theme	CHS	5362:5364	arg1	inhibitors					5366:5375	CHS inhibitors	5362:5375	CHS inhibitors	5362:5375	The development of this AOP will be useful in further research and regulatory initiatives related to assessment of CHS inhibitors and identification of critical knowledge gaps and may suggest new strategies for ecotoxicity testing efforts.
33818824	26	35	theme	term	4775:4778	arg1	"					4798:4798	The term "premature molting"	4771:4798	The term "premature molting"	4771:4798	The term "premature molting" is used to differentiate from the term "incomplete ecdysis," which describes inhibition of ecdysis on a behavioral level, namely through reduction of the EMP (Song et al. 2017a).
33818824	17	36	from	exoskeleton	3001:3011	arg1	insects					3016:3022	insects	3016:3022	insects	3016:3022	The shedding of the old exoskeleton in insects is mediated by a sequence of distinct muscular contractions, the ecdysis motor program (EMP; Ayali 2009; Song et al. 2017a).
33818824	3	37	theme	aquatic	497:503	arg1	invertebrates					505:517	aquatic invertebrates	497:517	aquatic invertebrates	497:517	Susceptible, nontarget organisms, such as insects and aquatic invertebrates, exposed to chitin synthesis inhibitors may suffer population declines, which may have a negative impact on ecosystems and associated services.
33818824	16	38	theme	chitin	2918:2923	arg1	synthesis					2925:2933	chitin synthesis	2918:2933	chitin synthesis	2918:2933	A detailed overview of the endocrine mechanisms regulating chitin synthesis is given in Supplemental Data, Figure S1.
33818824	9	39	theme	relevant	1615:1622	arg1	enzymes					1624:1630	relevant enzymes	1615:1630	relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors)	1615:1695	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	18	40	dep	Gagou	3322:3326	arg1	al.					3331:3333	Gagou et al.	3322:3333	Antoniewski et al. 1993; Gagou et al. 2002; Ayali 2009	3297:3350	Like the expression of chitin synthase isoform 1 (CHS-1), the expression of peptide hormones regulating the EMP is also controlled by ecdysteroids (Antoniewski et al. 1993; Gagou et al. 2002; Ayali 2009).
33818824	18	40	dep	Gagou	3322:3326	arg1	2002					3335:3338	2002	3335:3338	2002	3335:3338	Like the expression of chitin synthase isoform 1 (CHS-1), the expression of peptide hormones regulating the EMP is also controlled by ecdysteroids (Antoniewski et al. 1993; Gagou et al. 2002; Ayali 2009).
33818824	15	41	theme	determinant	2756:2766	arg1	chitin					2744:2749	chitin	2744:2749	chitin	2744:2749	Because the procuticle mainly consists of chitin microfibrils embedded in a matrix of cuticular proteins supplemented by lipids and minerals in insects (Muthukrishnan et al. 2012) and crustaceans (Cribb et al. 2009; Nagasawa 2012), chitin is a determinant factor for the appropriate composition of the cuticle and successful molting (Cohen 2001).
33818824	15	41	theme	determinant	2756:2766	arg1	factor					2768:2773	a determinant factor	2754:2773	a determinant factor for the appropriate composition of the cuticle and successful molting (Cohen 2001)	2754:2856	Because the procuticle mainly consists of chitin microfibrils embedded in a matrix of cuticular proteins supplemented by lipids and minerals in insects (Muthukrishnan et al. 2012) and crustaceans (Cribb et al. 2009; Nagasawa 2012), chitin is a determinant factor for the appropriate composition of the cuticle and successful molting (Cohen 2001).
33818824	18	42	theme	hormones	3233:3240	arg1	expression					3211:3220	the expression	3207:3220	the expression of peptide hormones regulating the EMP	3207:3259	Like the expression of chitin synthase isoform 1 (CHS-1), the expression of peptide hormones regulating the EMP is also controlled by ecdysteroids (Antoniewski et al. 1993; Gagou et al. 2002; Ayali 2009).
33818824	19	43	dep	insects	3535:3541	arg1	insects					3535:3541	insects	3535:3541	insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006)	3535:3629	Cuticular chitin is polymerized from uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) by the transmembrane enzyme CHS-1, which is localized in the epithelial plasma membrane in insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006).
33818824	19	43	dep	insects	3535:3541	arg1	Huie					3554:3557	Huie	3554:3557	Huie	3554:3557	Cuticular chitin is polymerized from uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) by the transmembrane enzyme CHS-1, which is localized in the epithelial plasma membrane in insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006).
33818824	19	43	dep	insects	3535:3541	arg1	Locke					3544:3548	Locke	3544:3548	Locke	3544:3548	Cuticular chitin is polymerized from uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) by the transmembrane enzyme CHS-1, which is localized in the epithelial plasma membrane in insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006).
33818824	25	44	dep	Camp	4733:4736	arg1	2017a					4763:4767	2014; Song et al. 2017a	4745:4767	Camp et al. 2014; Song et al. 2017a	4733:4767	These effects are observed in arthropods following molting, which fail to survive subsequent molts (Arakawa et al. 2008; Chen et al. 2008) or animals being stuck in their exuviae (Wang et al. 2019) and ultimately dying as a result of insufficient food or oxygen intake (Camp et al. 2014; Song et al. 2017a).
33818824	23	45	theme	ecdysis	4209:4215	arg1	cannot					4217:4222	ecdysis cannot	4209:4222	ecdysis cannot	4209:4222	If ecdysis cannot be completed because of decreased chitin synthesis, the organism may not successfully molt.
33818824	3	46	theme	synthesis	538:546	arg1	inhibitors					548:557	chitin synthesis inhibitors	531:557	chitin synthesis inhibitors	531:557	Susceptible, nontarget organisms, such as insects and aquatic invertebrates, exposed to chitin synthesis inhibitors may suffer population declines, which may have a negative impact on ecosystems and associated services.
33818824	21	47	theme	upstream	3959:3966	arg1	pathways					3978:3985	the upstream endocrine pathways	3955:3985	the upstream endocrine pathways	3955:3985	Disruption of either chitin synthesis or the upstream endocrine pathways can lead to lethal molting disruption (Arakawa et al. 2008; Merzendorfer et al. 2012; Song et al. 2017a, 2017b).
33818824	3	48	dep	population	570:579	arg1	declines					581:588	declines	581:588	declines	581:588	Susceptible, nontarget organisms, such as insects and aquatic invertebrates, exposed to chitin synthesis inhibitors may suffer population declines, which may have a negative impact on ecosystems and associated services.
33818824	14	49	theme	muscular	2468:2475	arg1	function					2477:2484	muscular function	2468:2484	muscular function	2468:2484	The cuticle protects arthropods from predators and desiccation, acts as a physical barrier against pathogens, and allows for locomotion by providing support for muscular function (Vincent and Wegst 2004).
33818824	25	50	theme	insufficient	4697:4708	arg1	food					4710:4713	insufficient food or oxygen intake	4697:4730	food	4710:4713	These effects are observed in arthropods following molting, which fail to survive subsequent molts (Arakawa et al. 2008; Chen et al. 2008) or animals being stuck in their exuviae (Wang et al. 2019) and ultimately dying as a result of insufficient food or oxygen intake (Camp et al. 2014; Song et al. 2017a).
33818824	17	51	from	shedding	2981:2988	arg1	insects					3016:3022	insects	3016:3022	insects	3016:3022	The shedding of the old exoskeleton in insects is mediated by a sequence of distinct muscular contractions, the ecdysis motor program (EMP; Ayali 2009; Song et al. 2017a).
33818824	21	52	theme	pathways	3978:3985	arg1	Disruption					3914:3923	Disruption	3914:3923	Disruption of either chitin synthesis or the upstream endocrine pathways	3914:3985	Disruption of either chitin synthesis or the upstream endocrine pathways can lead to lethal molting disruption (Arakawa et al. 2008; Merzendorfer et al. 2012; Song et al. 2017a, 2017b).
33818824	23	53	theme	chitin	4258:4263	arg1	synthesis					4265:4273	decreased chitin synthesis	4248:4273	decreased chitin synthesis	4248:4273	If ecdysis cannot be completed because of decreased chitin synthesis, the organism may not successfully molt.
33818824	21	54	theme	molting	4006:4012	arg1	Arakawa					4026:4032	Arakawa	4026:4032	Arakawa	4026:4032	Disruption of either chitin synthesis or the upstream endocrine pathways can lead to lethal molting disruption (Arakawa et al. 2008; Merzendorfer et al. 2012; Song et al. 2017a, 2017b).
33818824	21	54	theme	molting	4006:4012	arg1	disruption					4014:4023	lethal molting disruption	3999:4023	lethal molting disruption (Arakawa et al. 2008; Merzendorfer et al. 2012; Song et al. 2017a, 2017b)	3999:4097	Disruption of either chitin synthesis or the upstream endocrine pathways can lead to lethal molting disruption (Arakawa et al. 2008; Merzendorfer et al. 2012; Song et al. 2017a, 2017b).
33818824	9	55	theme	chitin	1639:1644	arg1	pathway					1659:1665	the chitin biosynthetic pathway	1635:1665	the chitin biosynthetic pathway (chitin synthesis inhibitors)	1635:1695	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	9	55	theme	chitin	1639:1644	arg1	inhibitors					1685:1694	chitin synthesis inhibitors	1668:1694	chitin synthesis inhibitors	1668:1694	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	12	56	theme	chitinous	2162:2170	arg1	procuticle					2172:2181	the underlying chitinous procuticle	2147:2181	the underlying chitinous procuticle	2147:2181	The arthropod exoskeleton (cuticle) can be divided into 2 layers, the thin and nonchitinous epicuticle, which is the outermost layer of the cuticle, and the underlying chitinous procuticle.
33818824	7	57	theme	approach	1262:1269	arg1	NAMs					1286:1289	NAMs	1286:1289	NAMs	1286:1289	The mechanistic understanding of the underlying toxicological processes leading to a regulation-relevant adverse outcome is necessary for the utilization of new approach methodologies (NAMs) and efficient coverage of wider chemical and taxonomic domains.
33818824	7	57	theme	approach	1262:1269	arg1	methodologies					1271:1283	new approach methodologies	1258:1283	new approach methodologies (NAMs)	1258:1290	The mechanistic understanding of the underlying toxicological processes leading to a regulation-relevant adverse outcome is necessary for the utilization of new approach methodologies (NAMs) and efficient coverage of wider chemical and taxonomic domains.
33818824	29	58	from	useful	5283:5288	arg1	initiatives					5325:5335	regulatory initiatives	5314:5335	regulatory initiatives related to assessment of CHS inhibitors and identification of critical knowledge gaps	5314:5421	The development of this AOP will be useful in further research and regulatory initiatives related to assessment of CHS inhibitors and identification of critical knowledge gaps and may suggest new strategies for ecotoxicity testing efforts.
33818824	29	58	from	useful	5283:5288	arg1	research					5301:5308	further research	5293:5308	further research	5293:5308	The development of this AOP will be useful in further research and regulatory initiatives related to assessment of CHS inhibitors and identification of critical knowledge gaps and may suggest new strategies for ecotoxicity testing efforts.
33818824	27	59	theme	direct	5042:5047	arg1	inhibition					5049:5058	direct inhibition	5042:5058	direct inhibition of the enzyme CHS-1	5042:5078	The present AOP describes molting-associated mortality through direct inhibition of the enzyme CHS-1.
33818824	11	60	theme	exoskeleton	1914:1924	arg1	synthesis					1895:1903	the synthesis	1891:1903	the synthesis of a new exoskeleton	1891:1924	This process is comprised of the synthesis of a new exoskeleton, followed by the exuviation of the old exoskeleton (Reynolds 1987).
33818824	21	61	theme	chitin	3935:3940	arg1	synthesis					3942:3950	chitin synthesis	3935:3950	chitin synthesis	3935:3950	Disruption of either chitin synthesis or the upstream endocrine pathways can lead to lethal molting disruption (Arakawa et al. 2008; Merzendorfer et al. 2012; Song et al. 2017a, 2017b).
33818824	32	62	theme	Wiley	5599:5603	arg1	LLC					5617:5619	Wiley Periodicals LLC	5599:5619	Wiley Periodicals LLC	5599:5619	Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
33818824	25	63	dep	Song	4751:4754	arg1	al.					4759:4761	Song et al.	4751:4761	Camp et al. 2014; Song et al. 2017a	4733:4767	These effects are observed in arthropods following molting, which fail to survive subsequent molts (Arakawa et al. 2008; Chen et al. 2008) or animals being stuck in their exuviae (Wang et al. 2019) and ultimately dying as a result of insufficient food or oxygen intake (Camp et al. 2014; Song et al. 2017a).
33818824	17	64	dep	program	3103:3109	arg1	EMP					3112:3114	EMP	3112:3114	EMP; Ayali 2009; Song et al. 2017a	3112:3145	The shedding of the old exoskeleton in insects is mediated by a sequence of distinct muscular contractions, the ecdysis motor program (EMP; Ayali 2009; Song et al. 2017a).
33818824	14	65	dep	providing	2446:2454	arg1	Vincent					2487:2493	Vincent	2487:2493	Vincent	2487:2493	The cuticle protects arthropods from predators and desiccation, acts as a physical barrier against pathogens, and allows for locomotion by providing support for muscular function (Vincent and Wegst 2004).
33818824	14	65	dep	providing	2446:2454	arg1	Wegst					2499:2503	Wegst	2499:2503	Wegst	2499:2503	The cuticle protects arthropods from predators and desiccation, acts as a physical barrier against pathogens, and allows for locomotion by providing support for muscular function (Vincent and Wegst 2004).
33818824	29	66	from	initiatives	5325:5335	arg1	development					5251:5261	The development	5247:5261	The development of this AOP	5247:5273	The development of this AOP will be useful in further research and regulatory initiatives related to assessment of CHS inhibitors and identification of critical knowledge gaps and may suggest new strategies for ecotoxicity testing efforts.
33818824	29	66	from	initiatives	5325:5335	arg1	useful					5283:5288	useful	5283:5288	useful	5283:5288	The development of this AOP will be useful in further research and regulatory initiatives related to assessment of CHS inhibitors and identification of critical knowledge gaps and may suggest new strategies for ecotoxicity testing efforts.
33818824	19	67	theme	enzyme	3465:3470	arg1	CHS-1					3472:3476	the transmembrane enzyme CHS-1	3447:3476	the transmembrane enzyme CHS-1	3447:3476	Cuticular chitin is polymerized from uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) by the transmembrane enzyme CHS-1, which is localized in the epithelial plasma membrane in insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006).
33818824	19	67	theme	enzyme	3465:3470	arg1	localized					3488:3496	localized	3488:3496	localized	3488:3496	Cuticular chitin is polymerized from uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) by the transmembrane enzyme CHS-1, which is localized in the epithelial plasma membrane in insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006).
33818824	5	68	theme	cost-efficient	822:835	arg1	approach					853:860	a more cost-efficient and mechanistic approach	815:860	a more cost-efficient and mechanistic approach in risk assessment	815:879	The need for a more cost-efficient and mechanistic approach in risk assessment has been clearly evident and triggered the development of the adverse outcome pathway (AOP) framework (Ankley et al. 2010).
33818824	9	69	theme	chitin	1668:1673	arg1	pathway					1659:1665	the chitin biosynthetic pathway	1635:1665	the chitin biosynthetic pathway (chitin synthesis inhibitors)	1635:1695	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	9	69	theme	chitin	1668:1673	arg1	inhibitors					1685:1694	chitin synthesis inhibitors	1668:1694	chitin synthesis inhibitors	1668:1694	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	25	70	dep	Arakawa	4563:4569	arg1	al.					4574:4576	Arakawa et al.	4563:4576	Arakawa et al. 2008	4563:4581	These effects are observed in arthropods following molting, which fail to survive subsequent molts (Arakawa et al. 2008; Chen et al. 2008) or animals being stuck in their exuviae (Wang et al. 2019) and ultimately dying as a result of insufficient food or oxygen intake (Camp et al. 2014; Song et al. 2017a).
33818824	25	70	dep	Arakawa	4563:4569	arg1	Chen					4584:4587	Chen	4584:4587	Chen	4584:4587	These effects are observed in arthropods following molting, which fail to survive subsequent molts (Arakawa et al. 2008; Chen et al. 2008) or animals being stuck in their exuviae (Wang et al. 2019) and ultimately dying as a result of insufficient food or oxygen intake (Camp et al. 2014; Song et al. 2017a).
33818824	25	70	dep	Arakawa	4563:4569	arg1	2008					4578:4581	2008	4578:4581	2008	4578:4581	These effects are observed in arthropods following molting, which fail to survive subsequent molts (Arakawa et al. 2008; Chen et al. 2008) or animals being stuck in their exuviae (Wang et al. 2019) and ultimately dying as a result of insufficient food or oxygen intake (Camp et al. 2014; Song et al. 2017a).
33818824	25	71	theme	food	4710:4713	arg1	result					4687:4692	a result	4685:4692	a result of insufficient food or oxygen intake (Camp et al. 2014; Song et al. 2017a)	4685:4768	These effects are observed in arthropods following molting, which fail to survive subsequent molts (Arakawa et al. 2008; Chen et al. 2008) or animals being stuck in their exuviae (Wang et al. 2019) and ultimately dying as a result of insufficient food or oxygen intake (Camp et al. 2014; Song et al. 2017a).
33818824	20	72	theme	CHS	3792:3794	arg1	isoforms					3796:3803	different CHS isoforms	3782:3803	different CHS isoforms	3782:3803	Because crustaceans are also dependent on the synthesis of chitin, the underlying mechanisms are believed to be similar, although less is known about different CHS isoforms and their localization (Rocha et al. 2012; Qian et al. 2014; Uddowla et al. 2014; Harðardóttir et al. 2019).
33818824	29	73	theme	AOP	5271:5273	arg1	development					5251:5261	The development	5247:5261	The development of this AOP	5247:5273	The development of this AOP will be useful in further research and regulatory initiatives related to assessment of CHS inhibitors and identification of critical knowledge gaps and may suggest new strategies for ecotoxicity testing efforts.
33818824	29	73	theme	AOP	5271:5273	arg1	useful					5283:5288	useful	5283:5288	useful	5283:5288	The development of this AOP will be useful in further research and regulatory initiatives related to assessment of CHS inhibitors and identification of critical knowledge gaps and may suggest new strategies for ecotoxicity testing efforts.
33818824	0	74	theme	Increased	55:63	arg1	Mortality					65:73	Increased Mortality	55:73	Increased Mortality in Arthropods	55:87	AOP Report: Inhibition of Chitin Synthase 1 Leading to Increased Mortality in Arthropods.
33818824	5	75	theme	mechanistic	841:851	arg1	approach					853:860	a more cost-efficient and mechanistic approach	815:860	a more cost-efficient and mechanistic approach in risk assessment	815:879	The need for a more cost-efficient and mechanistic approach in risk assessment has been clearly evident and triggered the development of the adverse outcome pathway (AOP) framework (Ankley et al. 2010).
33818824	9	76	from	enzymes	1624:1630	arg1	pathway					1659:1665	the chitin biosynthetic pathway	1635:1665	the chitin biosynthetic pathway (chitin synthesis inhibitors)	1635:1695	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	9	76	from	enzymes	1624:1630	arg1	inhibitors					1685:1694	chitin synthesis inhibitors	1668:1694	chitin synthesis inhibitors	1668:1694	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	7	77	theme	methodologies	1271:1283	arg1	coverage					1306:1313	efficient coverage	1296:1313	efficient coverage of wider chemical and taxonomic domains	1296:1353	The mechanistic understanding of the underlying toxicological processes leading to a regulation-relevant adverse outcome is necessary for the utilization of new approach methodologies (NAMs) and efficient coverage of wider chemical and taxonomic domains.
33818824	7	77	theme	methodologies	1271:1283	arg1	utilization					1243:1253	the utilization	1239:1253	the utilization of new approach methodologies (NAMs)	1239:1290	The mechanistic understanding of the underlying toxicological processes leading to a regulation-relevant adverse outcome is necessary for the utilization of new approach methodologies (NAMs) and efficient coverage of wider chemical and taxonomic domains.
33818824	25	78	theme	oxygen	4718:4723	arg1	intake					4725:4730	insufficient food or oxygen intake	4697:4730	intake	4725:4730	These effects are observed in arthropods following molting, which fail to survive subsequent molts (Arakawa et al. 2008; Chen et al. 2008) or animals being stuck in their exuviae (Wang et al. 2019) and ultimately dying as a result of insufficient food or oxygen intake (Camp et al. 2014; Song et al. 2017a).
33818824	17	79	theme	old	2997:2999	arg1	exoskeleton					3001:3011	the old exoskeleton	2993:3011	the old exoskeleton in insects	2993:3022	The shedding of the old exoskeleton in insects is mediated by a sequence of distinct muscular contractions, the ecdysis motor program (EMP; Ayali 2009; Song et al. 2017a).
33818824	29	80	theme	inhibitors	5366:5375	arg1	identification					5381:5394	identification	5381:5394	identification of critical knowledge gaps	5381:5421	The development of this AOP will be useful in further research and regulatory initiatives related to assessment of CHS inhibitors and identification of critical knowledge gaps and may suggest new strategies for ecotoxicity testing efforts.
33818824	29	80	theme	inhibitors	5366:5375	arg1	assessment					5348:5357	assessment	5348:5357	assessment of CHS inhibitors	5348:5375	The development of this AOP will be useful in further research and regulatory initiatives related to assessment of CHS inhibitors and identification of critical knowledge gaps and may suggest new strategies for ecotoxicity testing efforts.
33818824	2	81	dep	Junquera	420:427	arg1	al.					432:434	Junquera et al.	420:434	Merzendorfer 2013; Junquera et al. 2019	401:439	The highly conserved chitin synthetic process and the absence of this process in vertebrates make it an exploitable target for pest management and veterinary medicines (Merzendorfer 2013; Junquera et al. 2019).
33818824	2	81	dep	Junquera	420:427	arg1	2019					436:439	2019	436:439	2019	436:439	The highly conserved chitin synthetic process and the absence of this process in vertebrates make it an exploitable target for pest management and veterinary medicines (Merzendorfer 2013; Junquera et al. 2019).
33818824	17	82	dep	EMP	3112:3114	arg1	Ayali					3117:3121	Ayali	3117:3121	Ayali	3117:3121	The shedding of the old exoskeleton in insects is mediated by a sequence of distinct muscular contractions, the ecdysis motor program (EMP; Ayali 2009; Song et al. 2017a).
33818824	17	82	dep	EMP	3112:3114	arg1	2017a					3141:3145	Song et al. 2017a	3129:3145	EMP; Ayali 2009; Song et al. 2017a	3112:3145	The shedding of the old exoskeleton in insects is mediated by a sequence of distinct muscular contractions, the ecdysis motor program (EMP; Ayali 2009; Song et al. 2017a).
33818824	12	83	theme	outermost	2111:2119	arg1	epicuticle					2086:2095	the thin and nonchitinous epicuticle	2060:2095	the thin and nonchitinous epicuticle	2060:2095	The arthropod exoskeleton (cuticle) can be divided into 2 layers, the thin and nonchitinous epicuticle, which is the outermost layer of the cuticle, and the underlying chitinous procuticle.
33818824	12	83	theme	outermost	2111:2119	arg1	layer					2121:2125	the outermost layer	2107:2125	the outermost layer of the cuticle	2107:2140	The arthropod exoskeleton (cuticle) can be divided into 2 layers, the thin and nonchitinous epicuticle, which is the outermost layer of the cuticle, and the underlying chitinous procuticle.
33818824	12	83	theme	outermost	2111:2119	arg1	layers					2052:2057	2 layers	2050:2057	2 layers	2050:2057	The arthropod exoskeleton (cuticle) can be divided into 2 layers, the thin and nonchitinous epicuticle, which is the outermost layer of the cuticle, and the underlying chitinous procuticle.
33818824	17	84	theme	muscular	3062:3069	arg1	contractions					3071:3082	distinct muscular contractions	3053:3082	distinct muscular contractions	3053:3082	The shedding of the old exoskeleton in insects is mediated by a sequence of distinct muscular contractions, the ecdysis motor program (EMP; Ayali 2009; Song et al. 2017a).
33818824	17	84	theme	muscular	3062:3069	arg1	program					3103:3109	the ecdysis motor program	3085:3109	the ecdysis motor program (EMP; Ayali 2009; Song et al. 2017a)	3085:3146	The shedding of the old exoskeleton in insects is mediated by a sequence of distinct muscular contractions, the ecdysis motor program (EMP; Ayali 2009; Song et al. 2017a).
33818824	20	85	dep	Harðardóttir	3887:3898	arg1	2019					3907:3910	2019	3907:3910	2019	3907:3910	Because crustaceans are also dependent on the synthesis of chitin, the underlying mechanisms are believed to be similar, although less is known about different CHS isoforms and their localization (Rocha et al. 2012; Qian et al. 2014; Uddowla et al. 2014; Harðardóttir et al. 2019).
33818824	20	85	dep	Harðardóttir	3887:3898	arg1	al.					3903:3905	Harðardóttir et al.	3887:3905	Rocha et al. 2012; Qian et al. 2014; Uddowla et al. 2014; Harðardóttir et al. 2019	3829:3910	Because crustaceans are also dependent on the synthesis of chitin, the underlying mechanisms are believed to be similar, although less is known about different CHS isoforms and their localization (Rocha et al. 2012; Qian et al. 2014; Uddowla et al. 2014; Harðardóttir et al. 2019).
33818824	21	86	dep	Merzendorfer	4047:4058	arg1	al.					4063:4065	Merzendorfer et al.	4047:4065	Arakawa et al. 2008; Merzendorfer et al. 2012	4026:4070	Disruption of either chitin synthesis or the upstream endocrine pathways can lead to lethal molting disruption (Arakawa et al. 2008; Merzendorfer et al. 2012; Song et al. 2017a, 2017b).
33818824	21	86	dep	Merzendorfer	4047:4058	arg1	2012					4067:4070	2012	4067:4070	2012	4067:4070	Disruption of either chitin synthesis or the upstream endocrine pathways can lead to lethal molting disruption (Arakawa et al. 2008; Merzendorfer et al. 2012; Song et al. 2017a, 2017b).
33818824	12	87	theme	nonchitinous	2073:2084	arg1	epicuticle					2086:2095	the thin and nonchitinous epicuticle	2060:2095	the thin and nonchitinous epicuticle	2060:2095	The arthropod exoskeleton (cuticle) can be divided into 2 layers, the thin and nonchitinous epicuticle, which is the outermost layer of the cuticle, and the underlying chitinous procuticle.
33818824	12	87	theme	nonchitinous	2073:2084	arg1	layer					2121:2125	the outermost layer	2107:2125	the outermost layer of the cuticle	2107:2140	The arthropod exoskeleton (cuticle) can be divided into 2 layers, the thin and nonchitinous epicuticle, which is the outermost layer of the cuticle, and the underlying chitinous procuticle.
33818824	12	87	theme	nonchitinous	2073:2084	arg1	layers					2052:2057	2 layers	2050:2057	2 layers	2050:2057	The arthropod exoskeleton (cuticle) can be divided into 2 layers, the thin and nonchitinous epicuticle, which is the outermost layer of the cuticle, and the underlying chitinous procuticle.
33818824	0	88	theme	AOP	0:2	arg1	Report					4:9	AOP Report	0:9	AOP Report	0:9	AOP Report: Inhibition of Chitin Synthase 1 Leading to Increased Mortality in Arthropods.
33818824	5	89	from	approach	853:860	arg1	assessment					870:879	risk assessment	865:879	risk assessment	865:879	The need for a more cost-efficient and mechanistic approach in risk assessment has been clearly evident and triggered the development of the adverse outcome pathway (AOP) framework (Ankley et al. 2010).
33818824	29	90	from	research	5301:5308	arg1	development					5251:5261	The development	5247:5261	The development of this AOP	5247:5273	The development of this AOP will be useful in further research and regulatory initiatives related to assessment of CHS inhibitors and identification of critical knowledge gaps and may suggest new strategies for ecotoxicity testing efforts.
33818824	29	90	from	research	5301:5308	arg1	useful					5283:5288	useful	5283:5288	useful	5283:5288	The development of this AOP will be useful in further research and regulatory initiatives related to assessment of CHS inhibitors and identification of critical knowledge gaps and may suggest new strategies for ecotoxicity testing efforts.
33818824	28	91	dep	have	5150:5153	arg1	Bar-On					5226:5231	Bar-On	5226:5231	Bar-On	5226:5231	It expands the small but increasing number of invertebrate AOPs that have relevance to arthropods, the largest phylum within the animal kingdom (Bar-On et al. 2018).
33818824	9	92	theme	endocrine	1729:1737	arg1	systems					1739:1745	associated endocrine systems	1718:1745	associated endocrine systems	1718:1745	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	28	93	theme	animal	5210:5215	arg1	kingdom					5217:5223	the animal kingdom	5206:5223	the animal kingdom	5206:5223	It expands the small but increasing number of invertebrate AOPs that have relevance to arthropods, the largest phylum within the animal kingdom (Bar-On et al. 2018).
33818824	17	94	theme	ecdysis	3089:3095	arg1	contractions					3071:3082	distinct muscular contractions	3053:3082	distinct muscular contractions	3053:3082	The shedding of the old exoskeleton in insects is mediated by a sequence of distinct muscular contractions, the ecdysis motor program (EMP; Ayali 2009; Song et al. 2017a).
33818824	17	94	theme	ecdysis	3089:3095	arg1	program					3103:3109	the ecdysis motor program	3085:3109	the ecdysis motor program (EMP; Ayali 2009; Song et al. 2017a)	3085:3146	The shedding of the old exoskeleton in insects is mediated by a sequence of distinct muscular contractions, the ecdysis motor program (EMP; Ayali 2009; Song et al. 2017a).
33818824	32	95	theme	Periodicals	5605:5615	arg1	LLC					5617:5619	Wiley Periodicals LLC	5599:5619	Wiley Periodicals LLC	5599:5619	Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
33818824	26	96	used	used	4803:4806	arg2	"					4798:4798	The term "premature molting"	4771:4798	The term "premature molting"	4771:4798	The term "premature molting" is used to differentiate from the term "incomplete ecdysis," which describes inhibition of ecdysis on a behavioral level, namely through reduction of the EMP (Song et al. 2017a).
33818824	13	97	theme	layers	2284:2289	arg1	synthesis					2242:2250	synthesis	2242:2250	synthesis	2242:2250	A single layer of epithelial cells is responsible for the synthesis and secretion of both cuticular layers (Neville 1975).
33818824	13	97	theme	layers	2284:2289	arg1	secretion					2256:2264	secretion	2256:2264	secretion	2256:2264	A single layer of epithelial cells is responsible for the synthesis and secretion of both cuticular layers (Neville 1975).
33818824	25	98	theme	Song	4751:4754	arg1	2017a					4763:4767	2014; Song et al. 2017a	4745:4767	Camp et al. 2014; Song et al. 2017a	4733:4767	These effects are observed in arthropods following molting, which fail to survive subsequent molts (Arakawa et al. 2008; Chen et al. 2008) or animals being stuck in their exuviae (Wang et al. 2019) and ultimately dying as a result of insufficient food or oxygen intake (Camp et al. 2014; Song et al. 2017a).
33818824	9	99	with	inhibition	1601:1610	arg1	systems					1739:1745	associated endocrine systems	1718:1745	associated endocrine systems	1718:1745	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	28	100	contain	have	5150:5153	arg1	AOPs					5140:5143	invertebrate AOPs	5127:5143	invertebrate AOPs that have relevance to arthropods, the largest phylum within the animal kingdom (Bar-On et al. 2018)	5127:5244	It expands the small but increasing number of invertebrate AOPs that have relevance to arthropods, the largest phylum within the animal kingdom (Bar-On et al. 2018).
33818824	28	100	contain	have	5150:5153	arg2	relevance					5155:5163	relevance	5155:5163	relevance to arthropods	5155:5177	It expands the small but increasing number of invertebrate AOPs that have relevance to arthropods, the largest phylum within the animal kingdom (Bar-On et al. 2018).
33818824	28	100	contain	have	5150:5153	arg2	phylum					5192:5197	the largest phylum	5180:5197	the largest phylum within the animal kingdom	5180:5223	It expands the small but increasing number of invertebrate AOPs that have relevance to arthropods, the largest phylum within the animal kingdom (Bar-On et al. 2018).
33818824	2	101	theme	pest	359:362	arg1	management					364:373	pest management	359:373	pest management	359:373	The highly conserved chitin synthetic process and the absence of this process in vertebrates make it an exploitable target for pest management and veterinary medicines (Merzendorfer 2013; Junquera et al. 2019).
33818824	15	102	theme	embedded	2574:2581	arg1	microfibrils					2561:2572	chitin microfibrils	2554:2572	chitin microfibrils embedded in a matrix of cuticular proteins supplemented by lipids and minerals in insects (Muthukrishnan et al. 2012) and crustaceans (Cribb et al. 2009; Nagasawa 2012)	2554:2741	Because the procuticle mainly consists of chitin microfibrils embedded in a matrix of cuticular proteins supplemented by lipids and minerals in insects (Muthukrishnan et al. 2012) and crustaceans (Cribb et al. 2009; Nagasawa 2012), chitin is a determinant factor for the appropriate composition of the cuticle and successful molting (Cohen 2001).
33818824	26	103	dep	Song	4959:4962	arg1	al.					4967:4969	Song et al.	4959:4969	Song et al. 2017a	4959:4975	The term "premature molting" is used to differentiate from the term "incomplete ecdysis," which describes inhibition of ecdysis on a behavioral level, namely through reduction of the EMP (Song et al. 2017a).
33818824	14	104	theme	physical	2381:2388	arg1	cuticle					2311:2317	The cuticle	2307:2317	The cuticle	2307:2317	The cuticle protects arthropods from predators and desiccation, acts as a physical barrier against pathogens, and allows for locomotion by providing support for muscular function (Vincent and Wegst 2004).
33818824	14	104	theme	physical	2381:2388	arg1	barrier					2390:2396	a physical barrier	2379:2396	a physical barrier against pathogens	2379:2414	The cuticle protects arthropods from predators and desiccation, acts as a physical barrier against pathogens, and allows for locomotion by providing support for muscular function (Vincent and Wegst 2004).
33818824	26	105	theme	molting	4791:4797	arg1	"					4798:4798	The term "premature molting"	4771:4798	The term "premature molting"	4771:4798	The term "premature molting" is used to differentiate from the term "incomplete ecdysis," which describes inhibition of ecdysis on a behavioral level, namely through reduction of the EMP (Song et al. 2017a).
33818824	7	106	theme	adverse	1206:1212	arg1	outcome					1214:1220	a regulation-relevant adverse outcome	1184:1220	a regulation-relevant adverse outcome	1184:1220	The mechanistic understanding of the underlying toxicological processes leading to a regulation-relevant adverse outcome is necessary for the utilization of new approach methodologies (NAMs) and efficient coverage of wider chemical and taxonomic domains.
33818824	8	107	theme	research	1457:1464	arg1	community					1466:1474	the (eco)toxicological research community	1434:1474	the (eco)toxicological research community	1434:1474	In the last decade, the AOP framework has gained traction and expanded within the (eco)toxicological research community.
33818824	12	108	theme	thin	2064:2067	arg1	epicuticle					2086:2095	the thin and nonchitinous epicuticle	2060:2095	the thin and nonchitinous epicuticle	2060:2095	The arthropod exoskeleton (cuticle) can be divided into 2 layers, the thin and nonchitinous epicuticle, which is the outermost layer of the cuticle, and the underlying chitinous procuticle.
33818824	12	108	theme	thin	2064:2067	arg1	layer					2121:2125	the outermost layer	2107:2125	the outermost layer of the cuticle	2107:2140	The arthropod exoskeleton (cuticle) can be divided into 2 layers, the thin and nonchitinous epicuticle, which is the outermost layer of the cuticle, and the underlying chitinous procuticle.
33818824	12	108	theme	thin	2064:2067	arg1	layers					2052:2057	2 layers	2050:2057	2 layers	2050:2057	The arthropod exoskeleton (cuticle) can be divided into 2 layers, the thin and nonchitinous epicuticle, which is the outermost layer of the cuticle, and the underlying chitinous procuticle.
33818824	0	109	theme	Synthase	33:40	arg1	Inhibition					12:21	Inhibition	12:21	Inhibition of Chitin Synthase 1 Leading to Increased Mortality in Arthropods	12:87	AOP Report: Inhibition of Chitin Synthase 1 Leading to Increased Mortality in Arthropods.
33818824	26	110	dep	differentiate	4811:4823	arg1	Song					4959:4962	Song	4959:4962	Song	4959:4962	The term "premature molting" is used to differentiate from the term "incomplete ecdysis," which describes inhibition of ecdysis on a behavioral level, namely through reduction of the EMP (Song et al. 2017a).
33818824	26	110	dep	differentiate	4811:4823	arg1	2017a					4971:4975	2017a	4971:4975	2017a	4971:4975	The term "premature molting" is used to differentiate from the term "incomplete ecdysis," which describes inhibition of ecdysis on a behavioral level, namely through reduction of the EMP (Song et al. 2017a).
33818824	24	111	theme	chitin	4366:4371	arg1	synthesis					4373:4381	chitin synthesis	4366:4381	chitin synthesis	4366:4381	Even if ecdysis can be completed on inhibition of chitin synthesis, the organism may not survive because of the poor integrity of the new cuticle.
33818824	9	112	theme	endocrine	1775:1783	arg1	chemicals					1764:1772	environmental chemicals	1750:1772	environmental chemicals (endocrine disruptors)	1750:1795	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	9	112	theme	endocrine	1775:1783	arg1	disruptors					1785:1794	endocrine disruptors	1775:1794	endocrine disruptors	1775:1794	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	27	113	theme	enzyme	5067:5072	arg1	CHS-1					5074:5078	the enzyme CHS-1	5063:5078	the enzyme CHS-1	5063:5078	The present AOP describes molting-associated mortality through direct inhibition of the enzyme CHS-1.
33818824	26	114	theme	ecdysis	4891:4897	arg1	inhibition					4877:4886	inhibition	4877:4886	inhibition of ecdysis	4877:4897	The term "premature molting" is used to differentiate from the term "incomplete ecdysis," which describes inhibition of ecdysis on a behavioral level, namely through reduction of the EMP (Song et al. 2017a).
33818824	24	115	theme	synthesis	4373:4381	arg1	inhibition					4352:4361	inhibition	4352:4361	inhibition of chitin synthesis	4352:4381	Even if ecdysis can be completed on inhibition of chitin synthesis, the organism may not survive because of the poor integrity of the new cuticle.
33818824	29	116	theme	testing	5470:5476	arg1	efforts					5478:5484	ecotoxicity testing efforts	5458:5484	ecotoxicity testing efforts	5458:5484	The development of this AOP will be useful in further research and regulatory initiatives related to assessment of CHS inhibitors and identification of critical knowledge gaps and may suggest new strategies for ecotoxicity testing efforts.
33818824	19	117	theme	transmembrane	3451:3463	arg1	CHS-1					3472:3476	the transmembrane enzyme CHS-1	3447:3476	the transmembrane enzyme CHS-1	3447:3476	Cuticular chitin is polymerized from uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) by the transmembrane enzyme CHS-1, which is localized in the epithelial plasma membrane in insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006).
33818824	19	117	theme	transmembrane	3451:3463	arg1	localized					3488:3496	localized	3488:3496	localized	3488:3496	Cuticular chitin is polymerized from uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) by the transmembrane enzyme CHS-1, which is localized in the epithelial plasma membrane in insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006).
33818824	9	118	from	pathway	1659:1665	arg1	inhibition					1601:1610	direct inhibition	1594:1610	direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors)	1594:1695	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	9	118	from	pathway	1659:1665	arg1	interference					1700:1711	interference	1700:1711	interference with associated endocrine systems by environmental chemicals (endocrine disruptors)	1700:1795	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	6	119	theme	molecular	1020:1028	arg1	MIE					1048:1050	MIE	1048:1050	MIE	1048:1050	An AOP links a molecular initiating event (MIE) through key events (KEs) to an adverse outcome.
33818824	6	119	theme	molecular	1020:1028	arg1	event					1041:1045	a molecular initiating event	1018:1045	a molecular initiating event (MIE)	1018:1051	An AOP links a molecular initiating event (MIE) through key events (KEs) to an adverse outcome.
33818824	28	120	theme	invertebrate	5127:5138	arg1	AOPs					5140:5143	invertebrate AOPs	5127:5143	invertebrate AOPs that have relevance to arthropods, the largest phylum within the animal kingdom (Bar-On et al. 2018)	5127:5244	It expands the small but increasing number of invertebrate AOPs that have relevance to arthropods, the largest phylum within the animal kingdom (Bar-On et al. 2018).
33818824	17	121	from	insects	3016:3022	arg1	shedding					2981:2988	The shedding	2977:2988	The shedding of the old exoskeleton in insects	2977:3022	The shedding of the old exoskeleton in insects is mediated by a sequence of distinct muscular contractions, the ecdysis motor program (EMP; Ayali 2009; Song et al. 2017a).
33818824	27	122	theme	present	4983:4989	arg1	AOP					4991:4993	The present AOP	4979:4993	The present AOP	4979:4993	The present AOP describes molting-associated mortality through direct inhibition of the enzyme CHS-1.
33818824	4	123	theme	relevant	733:740	arg1	chemicals					742:750	relevant chemicals	733:750	relevant chemicals posing potential hazards to nontarget arthropods	733:799	Hence, it is important to properly identify, prioritize, and regulate relevant chemicals posing potential hazards to nontarget arthropods.
33818824	20	124	dep	Rocha	3829:3833	arg1	al.					3838:3840	Rocha et al.	3829:3840	Rocha et al. 2012; Qian et al. 2014; Uddowla et al. 2014; Harðardóttir et al. 2019	3829:3910	Because crustaceans are also dependent on the synthesis of chitin, the underlying mechanisms are believed to be similar, although less is known about different CHS isoforms and their localization (Rocha et al. 2012; Qian et al. 2014; Uddowla et al. 2014; Harðardóttir et al. 2019).
33818824	20	124	dep	Rocha	3829:3833	arg1	Qian					3848:3851	Qian	3848:3851	Qian	3848:3851	Because crustaceans are also dependent on the synthesis of chitin, the underlying mechanisms are believed to be similar, although less is known about different CHS isoforms and their localization (Rocha et al. 2012; Qian et al. 2014; Uddowla et al. 2014; Harðardóttir et al. 2019).
33818824	20	124	dep	Rocha	3829:3833	arg1	2012					3842:3845	2012	3842:3845	2012	3842:3845	Because crustaceans are also dependent on the synthesis of chitin, the underlying mechanisms are believed to be similar, although less is known about different CHS isoforms and their localization (Rocha et al. 2012; Qian et al. 2014; Uddowla et al. 2014; Harðardóttir et al. 2019).
33818824	20	124	dep	Rocha	3829:3833	arg1	Uddowla					3866:3872	Uddowla	3866:3872	Uddowla	3866:3872	Because crustaceans are also dependent on the synthesis of chitin, the underlying mechanisms are believed to be similar, although less is known about different CHS isoforms and their localization (Rocha et al. 2012; Qian et al. 2014; Uddowla et al. 2014; Harðardóttir et al. 2019).
33818824	20	124	dep	Rocha	3829:3833	arg1	Harðardóttir					3887:3898	Harðardóttir	3887:3898	Harðardóttir	3887:3898	Because crustaceans are also dependent on the synthesis of chitin, the underlying mechanisms are believed to be similar, although less is known about different CHS isoforms and their localization (Rocha et al. 2012; Qian et al. 2014; Uddowla et al. 2014; Harðardóttir et al. 2019).
33818824	1	125	theme	life	199:202	arg1	cycles					204:209	their life cycles	193:209	their life cycles (Merzendorfer 2011)	193:229	Arthropods (including insects, crustaceans, and arachnids) rely on the synthesis of chitin to complete their life cycles (Merzendorfer 2011).
33818824	1	125	theme	life	199:202	arg1	2011					225:228	Merzendorfer 2011	212:228	Merzendorfer 2011	212:228	Arthropods (including insects, crustaceans, and arachnids) rely on the synthesis of chitin to complete their life cycles (Merzendorfer 2011).
33818824	9	126	theme	invertebrate	1516:1527	arg1	AOPs					1529:1532	mature invertebrate AOPs	1509:1532	mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors)	1509:1795	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	7	127	theme	toxicological	1149:1161	arg1	processes					1163:1171	the underlying toxicological processes	1134:1171	the underlying toxicological processes leading to a regulation-relevant adverse outcome	1134:1220	The mechanistic understanding of the underlying toxicological processes leading to a regulation-relevant adverse outcome is necessary for the utilization of new approach methodologies (NAMs) and efficient coverage of wider chemical and taxonomic domains.
33818824	28	128	theme	largest	5184:5190	arg1	relevance					5155:5163	relevance	5155:5163	relevance to arthropods	5155:5177	It expands the small but increasing number of invertebrate AOPs that have relevance to arthropods, the largest phylum within the animal kingdom (Bar-On et al. 2018).
33818824	28	128	theme	largest	5184:5190	arg1	phylum					5192:5197	the largest phylum	5180:5197	the largest phylum within the animal kingdom	5180:5223	It expands the small but increasing number of invertebrate AOPs that have relevance to arthropods, the largest phylum within the animal kingdom (Bar-On et al. 2018).
33818824	29	129	theme	gaps	5418:5421	arg1	identification					5381:5394	identification	5381:5394	identification of critical knowledge gaps	5381:5421	The development of this AOP will be useful in further research and regulatory initiatives related to assessment of CHS inhibitors and identification of critical knowledge gaps and may suggest new strategies for ecotoxicity testing efforts.
33818824	29	129	theme	gaps	5418:5421	arg1	assessment					5348:5357	assessment	5348:5357	assessment of CHS inhibitors	5348:5375	The development of this AOP will be useful in further research and regulatory initiatives related to assessment of CHS inhibitors and identification of critical knowledge gaps and may suggest new strategies for ecotoxicity testing efforts.
33818824	3	130	theme	associated	642:651	arg1	services					653:660	associated services	642:660	associated services	642:660	Susceptible, nontarget organisms, such as insects and aquatic invertebrates, exposed to chitin synthesis inhibitors may suffer population declines, which may have a negative impact on ecosystems and associated services.
33818824	3	131	theme	Susceptible	443:453	arg1	insects					485:491	insects	485:491	insects	485:491	Susceptible, nontarget organisms, such as insects and aquatic invertebrates, exposed to chitin synthesis inhibitors may suffer population declines, which may have a negative impact on ecosystems and associated services.
33818824	3	131	theme	Susceptible	443:453	arg1	invertebrates					505:517	aquatic invertebrates	497:517	aquatic invertebrates	497:517	Susceptible, nontarget organisms, such as insects and aquatic invertebrates, exposed to chitin synthesis inhibitors may suffer population declines, which may have a negative impact on ecosystems and associated services.
33818824	3	131	theme	Susceptible	443:453	arg1	organisms					466:474	Susceptible, nontarget organisms	443:474	Susceptible, nontarget organisms	443:474	Susceptible, nontarget organisms, such as insects and aquatic invertebrates, exposed to chitin synthesis inhibitors may suffer population declines, which may have a negative impact on ecosystems and associated services.
33818824	16	132	theme	Supplemental	2947:2958	arg1	Data					2960:2963	Supplemental Data	2947:2963	Supplemental Data	2947:2963	A detailed overview of the endocrine mechanisms regulating chitin synthesis is given in Supplemental Data, Figure S1.
33818824	16	132	theme	Supplemental	2947:2958	arg1	S1					2973:2974	Figure S1	2966:2974	Figure S1	2966:2974	A detailed overview of the endocrine mechanisms regulating chitin synthesis is given in Supplemental Data, Figure S1.
33818824	2	133	from	absence	286:292	arg1	vertebrates					313:323	vertebrates	313:323	vertebrates	313:323	The highly conserved chitin synthetic process and the absence of this process in vertebrates make it an exploitable target for pest management and veterinary medicines (Merzendorfer 2013; Junquera et al. 2019).
33818824	28	134	theme	AOPs	5140:5143	arg1	number					5117:5122	the small but increasing number	5092:5122	the small but increasing number of invertebrate AOPs that have relevance to arthropods, the largest phylum within the animal kingdom (Bar-On et al. 2018)	5092:5244	It expands the small but increasing number of invertebrate AOPs that have relevance to arthropods, the largest phylum within the animal kingdom (Bar-On et al. 2018).
33818824	15	135	dep	insects	2656:2662	arg1	Muthukrishnan					2665:2677	Muthukrishnan	2665:2677	Muthukrishnan	2665:2677	Because the procuticle mainly consists of chitin microfibrils embedded in a matrix of cuticular proteins supplemented by lipids and minerals in insects (Muthukrishnan et al. 2012) and crustaceans (Cribb et al. 2009; Nagasawa 2012), chitin is a determinant factor for the appropriate composition of the cuticle and successful molting (Cohen 2001).
33818824	19	136	theme	epithelial	3505:3514	arg1	membrane					3523:3530	the epithelial plasma membrane	3501:3530	the epithelial plasma membrane in insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006)	3501:3629	Cuticular chitin is polymerized from uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) by the transmembrane enzyme CHS-1, which is localized in the epithelial plasma membrane in insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006).
33818824	2	137	theme	conserved	243:251	arg1	process					270:276	The highly conserved chitin synthetic process	232:276	The highly conserved chitin synthetic process	232:276	The highly conserved chitin synthetic process and the absence of this process in vertebrates make it an exploitable target for pest management and veterinary medicines (Merzendorfer 2013; Junquera et al. 2019).
33818824	22	138	theme	premature	4186:4194	arg1	molting					4196:4202	"premature molting	4185:4202	"premature molting	4185:4202	In the case of chitin synthesis inhibition, molting disruption can be referred to as "premature molting."
33818824	22	138	theme	premature	4186:4194	arg1	disruption					4152:4161	molting disruption	4144:4161	molting disruption	4144:4161	In the case of chitin synthesis inhibition, molting disruption can be referred to as "premature molting."
33818824	25	139	located	observed	4481:4488	arg1	arthropods					4493:4502	arthropods	4493:4502	arthropods	4493:4502	These effects are observed in arthropods following molting, which fail to survive subsequent molts (Arakawa et al. 2008; Chen et al. 2008) or animals being stuck in their exuviae (Wang et al. 2019) and ultimately dying as a result of insufficient food or oxygen intake (Camp et al. 2014; Song et al. 2017a).
33818824	25	139	located	observed	4481:4488	arg2	effects					4469:4475	These effects	4463:4475	These effects	4463:4475	These effects are observed in arthropods following molting, which fail to survive subsequent molts (Arakawa et al. 2008; Chen et al. 2008) or animals being stuck in their exuviae (Wang et al. 2019) and ultimately dying as a result of insufficient food or oxygen intake (Camp et al. 2014; Song et al. 2017a).
33818824	28	140	dep	Bar-On	5226:5231	arg1	2018					5240:5243	2018	5240:5243	2018	5240:5243	It expands the small but increasing number of invertebrate AOPs that have relevance to arthropods, the largest phylum within the animal kingdom (Bar-On et al. 2018).
33818824	28	140	dep	Bar-On	5226:5231	arg1	al.					5236:5238	Bar-On et al.	5226:5238	Bar-On et al. 2018	5226:5243	It expands the small but increasing number of invertebrate AOPs that have relevance to arthropods, the largest phylum within the animal kingdom (Bar-On et al. 2018).
33818824	9	141	theme	defect-associated	1553:1569	arg1	mortality					1571:1579	molting defect-associated mortality	1545:1579	molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors)	1545:1795	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	7	142	theme	domains	1347:1353	arg1	coverage					1306:1313	efficient coverage	1296:1313	efficient coverage of wider chemical and taxonomic domains	1296:1353	The mechanistic understanding of the underlying toxicological processes leading to a regulation-relevant adverse outcome is necessary for the utilization of new approach methodologies (NAMs) and efficient coverage of wider chemical and taxonomic domains.
33818824	7	142	theme	domains	1347:1353	arg1	utilization					1243:1253	the utilization	1239:1253	the utilization of new approach methodologies (NAMs)	1239:1290	The mechanistic understanding of the underlying toxicological processes leading to a regulation-relevant adverse outcome is necessary for the utilization of new approach methodologies (NAMs) and efficient coverage of wider chemical and taxonomic domains.
33818824	18	143	theme	peptide	3225:3231	arg1	hormones					3233:3240	peptide hormones	3225:3240	peptide hormones regulating the EMP	3225:3259	Like the expression of chitin synthase isoform 1 (CHS-1), the expression of peptide hormones regulating the EMP is also controlled by ecdysteroids (Antoniewski et al. 1993; Gagou et al. 2002; Ayali 2009).
33818824	15	144	dep	Muthukrishnan	2665:2677	arg1	2012					2686:2689	2012	2686:2689	2012	2686:2689	Because the procuticle mainly consists of chitin microfibrils embedded in a matrix of cuticular proteins supplemented by lipids and minerals in insects (Muthukrishnan et al. 2012) and crustaceans (Cribb et al. 2009; Nagasawa 2012), chitin is a determinant factor for the appropriate composition of the cuticle and successful molting (Cohen 2001).
33818824	15	144	dep	Muthukrishnan	2665:2677	arg1	al.					2682:2684	Muthukrishnan et al.	2665:2684	Muthukrishnan et al. 2012	2665:2689	Because the procuticle mainly consists of chitin microfibrils embedded in a matrix of cuticular proteins supplemented by lipids and minerals in insects (Muthukrishnan et al. 2012) and crustaceans (Cribb et al. 2009; Nagasawa 2012), chitin is a determinant factor for the appropriate composition of the cuticle and successful molting (Cohen 2001).
33818824	26	145	theme	EMP	4954:4956	arg1	reduction					4937:4945	reduction	4937:4945	reduction of the EMP	4937:4956	The term "premature molting" is used to differentiate from the term "incomplete ecdysis," which describes inhibition of ecdysis on a behavioral level, namely through reduction of the EMP (Song et al. 2017a).
33818824	16	146	theme	detailed	2861:2868	arg1	overview					2870:2877	A detailed overview	2859:2877	A detailed overview of the endocrine mechanisms regulating chitin synthesis	2859:2933	A detailed overview of the endocrine mechanisms regulating chitin synthesis is given in Supplemental Data, Figure S1.
33818824	13	147	theme	single	2186:2191	arg1	responsible					2222:2232	responsible	2222:2232	responsible	2222:2232	A single layer of epithelial cells is responsible for the synthesis and secretion of both cuticular layers (Neville 1975).
33818824	13	147	theme	single	2186:2191	arg1	layer					2193:2197	A single layer	2184:2197	A single layer of epithelial cells	2184:2217	A single layer of epithelial cells is responsible for the synthesis and secretion of both cuticular layers (Neville 1975).
33818824	13	147	theme	single	2186:2191	arg1	Neville					2292:2298	Neville	2292:2298	Neville	2292:2298	A single layer of epithelial cells is responsible for the synthesis and secretion of both cuticular layers (Neville 1975).
33818824	15	148	theme	chitin	2554:2559	arg1	microfibrils					2561:2572	chitin microfibrils	2554:2572	chitin microfibrils embedded in a matrix of cuticular proteins supplemented by lipids and minerals in insects (Muthukrishnan et al. 2012) and crustaceans (Cribb et al. 2009; Nagasawa 2012)	2554:2741	Because the procuticle mainly consists of chitin microfibrils embedded in a matrix of cuticular proteins supplemented by lipids and minerals in insects (Muthukrishnan et al. 2012) and crustaceans (Cribb et al. 2009; Nagasawa 2012), chitin is a determinant factor for the appropriate composition of the cuticle and successful molting (Cohen 2001).
33818824	3	149	contain	have	601:604	arg2	impact					617:622	a negative impact	606:622	a negative impact	606:622	Susceptible, nontarget organisms, such as insects and aquatic invertebrates, exposed to chitin synthesis inhibitors may suffer population declines, which may have a negative impact on ecosystems and associated services.
33818824	3	149	contain	have	601:604	arg1	population					570:579	population declines	570:588	population declines	570:588	Susceptible, nontarget organisms, such as insects and aquatic invertebrates, exposed to chitin synthesis inhibitors may suffer population declines, which may have a negative impact on ecosystems and associated services.
33818824	18	150	theme	chitin	3172:3177	arg1	isoform					3188:3194	chitin synthase isoform 1	3172:3196	chitin synthase isoform 1 (CHS-1)	3172:3204	Like the expression of chitin synthase isoform 1 (CHS-1), the expression of peptide hormones regulating the EMP is also controlled by ecdysteroids (Antoniewski et al. 1993; Gagou et al. 2002; Ayali 2009).
33818824	18	150	theme	chitin	3172:3177	arg1	CHS-1					3199:3203	CHS-1	3199:3203	CHS-1	3199:3203	Like the expression of chitin synthase isoform 1 (CHS-1), the expression of peptide hormones regulating the EMP is also controlled by ecdysteroids (Antoniewski et al. 1993; Gagou et al. 2002; Ayali 2009).
33818824	15	151	theme	cuticular	2598:2606	arg1	proteins					2608:2615	cuticular proteins	2598:2615	cuticular proteins supplemented by lipids and minerals in insects (Muthukrishnan et al. 2012) and crustaceans (Cribb et al. 2009; Nagasawa 2012)	2598:2741	Because the procuticle mainly consists of chitin microfibrils embedded in a matrix of cuticular proteins supplemented by lipids and minerals in insects (Muthukrishnan et al. 2012) and crustaceans (Cribb et al. 2009; Nagasawa 2012), chitin is a determinant factor for the appropriate composition of the cuticle and successful molting (Cohen 2001).
33818824	24	152	theme	poor	4428:4431	arg1	integrity					4433:4441	the poor integrity	4424:4441	the poor integrity of the new cuticle	4424:4460	Even if ecdysis can be completed on inhibition of chitin synthesis, the organism may not survive because of the poor integrity of the new cuticle.
33818824	9	153	theme	direct	1594:1599	arg1	inhibition					1601:1610	direct inhibition	1594:1610	direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors)	1594:1695	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	13	154	dep	synthesis	2242:2250	arg1	the					2238:2240	the	2238:2240	the	2238:2240	A single layer of epithelial cells is responsible for the synthesis and secretion of both cuticular layers (Neville 1975).
33818824	2	155	theme	synthetic	260:268	arg1	process					270:276	The highly conserved chitin synthetic process	232:276	The highly conserved chitin synthetic process	232:276	The highly conserved chitin synthetic process and the absence of this process in vertebrates make it an exploitable target for pest management and veterinary medicines (Merzendorfer 2013; Junquera et al. 2019).
33818824	16	156	theme	endocrine	2886:2894	arg1	mechanisms					2896:2905	the endocrine mechanisms	2882:2905	the endocrine mechanisms regulating chitin synthesis	2882:2933	A detailed overview of the endocrine mechanisms regulating chitin synthesis is given in Supplemental Data, Figure S1.
33818824	29	157	theme	related	5337:5343	arg1	initiatives					5325:5335	regulatory initiatives	5314:5335	regulatory initiatives related to assessment of CHS inhibitors and identification of critical knowledge gaps	5314:5421	The development of this AOP will be useful in further research and regulatory initiatives related to assessment of CHS inhibitors and identification of critical knowledge gaps and may suggest new strategies for ecotoxicity testing efforts.
33818824	11	158	theme	exoskeleton	1965:1975	arg1	exuviation					1943:1952	the exuviation	1939:1952	the exuviation of the old exoskeleton	1939:1975	This process is comprised of the synthesis of a new exoskeleton, followed by the exuviation of the old exoskeleton (Reynolds 1987).
33818824	0	159	from	Mortality	65:73	arg1	Arthropods					78:87	Arthropods	78:87	Arthropods	78:87	AOP Report: Inhibition of Chitin Synthase 1 Leading to Increased Mortality in Arthropods.
33818824	5	160	dep	outcome	951:957	arg1	framework					973:981	pathway (AOP) framework	959:981	the adverse outcome pathway (AOP) framework (Ankley et al. 2010)	939:1002	The need for a more cost-efficient and mechanistic approach in risk assessment has been clearly evident and triggered the development of the adverse outcome pathway (AOP) framework (Ankley et al. 2010).
33818824	31	161	theme	©	5527:5527	arg1	2021					5529:5532	© 2021	5527:5532	© 2021	5527:5532	© 2021 The Authors.
33818824	3	162	theme	chitin	531:536	arg1	inhibitors					548:557	chitin synthesis inhibitors	531:557	chitin synthesis inhibitors	531:557	Susceptible, nontarget organisms, such as insects and aquatic invertebrates, exposed to chitin synthesis inhibitors may suffer population declines, which may have a negative impact on ecosystems and associated services.
33818824	13	163	theme	epithelial	2202:2211	arg1	cells					2213:2217	epithelial cells	2202:2217	epithelial cells	2202:2217	A single layer of epithelial cells is responsible for the synthesis and secretion of both cuticular layers (Neville 1975).
33818824	1	164	theme	chitin	174:179	arg1	synthesis					161:169	the synthesis	157:169	the synthesis of chitin	157:179	Arthropods (including insects, crustaceans, and arachnids) rely on the synthesis of chitin to complete their life cycles (Merzendorfer 2011).
33818824	21	165	dep	Arakawa	4026:4032	arg1	2017b					4092:4096	2017b	4092:4096	2017b	4092:4096	Disruption of either chitin synthesis or the upstream endocrine pathways can lead to lethal molting disruption (Arakawa et al. 2008; Merzendorfer et al. 2012; Song et al. 2017a, 2017b).
33818824	21	165	dep	Arakawa	4026:4032	arg1	Song					4073:4076	Song	4073:4076	Song	4073:4076	Disruption of either chitin synthesis or the upstream endocrine pathways can lead to lethal molting disruption (Arakawa et al. 2008; Merzendorfer et al. 2012; Song et al. 2017a, 2017b).
33818824	21	165	dep	Arakawa	4026:4032	arg1	Merzendorfer					4047:4058	Merzendorfer	4047:4058	Merzendorfer	4047:4058	Disruption of either chitin synthesis or the upstream endocrine pathways can lead to lethal molting disruption (Arakawa et al. 2008; Merzendorfer et al. 2012; Song et al. 2017a, 2017b).
33818824	20	166	dep	Qian	3848:3851	arg1	2014					3860:3863	2014	3860:3863	2014	3860:3863	Because crustaceans are also dependent on the synthesis of chitin, the underlying mechanisms are believed to be similar, although less is known about different CHS isoforms and their localization (Rocha et al. 2012; Qian et al. 2014; Uddowla et al. 2014; Harðardóttir et al. 2019).
33818824	20	166	dep	Qian	3848:3851	arg1	al.					3856:3858	Qian et al.	3848:3858	Rocha et al. 2012; Qian et al. 2014; Uddowla et al. 2014; Harðardóttir et al. 2019	3829:3910	Because crustaceans are also dependent on the synthesis of chitin, the underlying mechanisms are believed to be similar, although less is known about different CHS isoforms and their localization (Rocha et al. 2012; Qian et al. 2014; Uddowla et al. 2014; Harðardóttir et al. 2019).
33818824	9	167	theme	enzymes	1624:1630	arg1	inhibition					1601:1610	direct inhibition	1594:1610	direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors)	1594:1695	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	9	167	theme	enzymes	1624:1630	arg1	interference					1700:1711	interference	1700:1711	interference with associated endocrine systems by environmental chemicals (endocrine disruptors)	1700:1795	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	8	168	theme	last	1363:1366	arg1	decade					1368:1373	the last decade	1359:1373	the last decade	1359:1373	In the last decade, the AOP framework has gained traction and expanded within the (eco)toxicological research community.
33818824	12	169	theme	arthropod	1998:2006	arg1	cuticle					2021:2027	cuticle	2021:2027	cuticle	2021:2027	The arthropod exoskeleton (cuticle) can be divided into 2 layers, the thin and nonchitinous epicuticle, which is the outermost layer of the cuticle, and the underlying chitinous procuticle.
33818824	12	169	theme	arthropod	1998:2006	arg1	exoskeleton					2008:2018	The arthropod exoskeleton	1994:2018	The arthropod exoskeleton (cuticle)	1994:2028	The arthropod exoskeleton (cuticle) can be divided into 2 layers, the thin and nonchitinous epicuticle, which is the outermost layer of the cuticle, and the underlying chitinous procuticle.
33818824	21	170	theme	endocrine	3968:3976	arg1	pathways					3978:3985	the upstream endocrine pathways	3955:3985	the upstream endocrine pathways	3955:3985	Disruption of either chitin synthesis or the upstream endocrine pathways can lead to lethal molting disruption (Arakawa et al. 2008; Merzendorfer et al. 2012; Song et al. 2017a, 2017b).
33818824	7	171	dep	chemical	1324:1331	arg1	wider					1318:1322	wider	1318:1322	wider	1318:1322	The mechanistic understanding of the underlying toxicological processes leading to a regulation-relevant adverse outcome is necessary for the utilization of new approach methodologies (NAMs) and efficient coverage of wider chemical and taxonomic domains.
33818824	15	172	from	embedded	2574:2581	arg1	matrix					2588:2593	a matrix	2586:2593	a matrix of cuticular proteins supplemented by lipids and minerals in insects (Muthukrishnan et al. 2012) and crustaceans (Cribb et al. 2009; Nagasawa 2012)	2586:2741	Because the procuticle mainly consists of chitin microfibrils embedded in a matrix of cuticular proteins supplemented by lipids and minerals in insects (Muthukrishnan et al. 2012) and crustaceans (Cribb et al. 2009; Nagasawa 2012), chitin is a determinant factor for the appropriate composition of the cuticle and successful molting (Cohen 2001).
33818824	20	173	theme	chitin	3691:3696	arg1	synthesis					3678:3686	the synthesis	3674:3686	the synthesis of chitin	3674:3696	Because crustaceans are also dependent on the synthesis of chitin, the underlying mechanisms are believed to be similar, although less is known about different CHS isoforms and their localization (Rocha et al. 2012; Qian et al. 2014; Uddowla et al. 2014; Harðardóttir et al. 2019).
33818824	19	174	from	membrane	3523:3530	arg1	CHS-1					3472:3476	the transmembrane enzyme CHS-1	3447:3476	the transmembrane enzyme CHS-1	3447:3476	Cuticular chitin is polymerized from uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) by the transmembrane enzyme CHS-1, which is localized in the epithelial plasma membrane in insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006).
33818824	19	174	from	membrane	3523:3530	arg1	insects					3535:3541	insects	3535:3541	insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006)	3535:3629	Cuticular chitin is polymerized from uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) by the transmembrane enzyme CHS-1, which is localized in the epithelial plasma membrane in insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006).
33818824	19	174	from	membrane	3523:3530	arg1	localized					3488:3496	localized	3488:3496	localized	3488:3496	Cuticular chitin is polymerized from uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) by the transmembrane enzyme CHS-1, which is localized in the epithelial plasma membrane in insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006).
33818824	19	174	from	membrane	3523:3530	arg1	Huie					3554:3557	Huie	3554:3557	Huie	3554:3557	Cuticular chitin is polymerized from uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) by the transmembrane enzyme CHS-1, which is localized in the epithelial plasma membrane in insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006).
33818824	19	174	from	membrane	3523:3530	arg1	Locke					3544:3548	Locke	3544:3548	Locke	3544:3548	Cuticular chitin is polymerized from uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) by the transmembrane enzyme CHS-1, which is localized in the epithelial plasma membrane in insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006).
33818824	13	175	theme	cells	2213:2217	arg1	responsible					2222:2232	responsible	2222:2232	responsible	2222:2232	A single layer of epithelial cells is responsible for the synthesis and secretion of both cuticular layers (Neville 1975).
33818824	13	175	theme	cells	2213:2217	arg1	layer					2193:2197	A single layer	2184:2197	A single layer of epithelial cells	2184:2217	A single layer of epithelial cells is responsible for the synthesis and secretion of both cuticular layers (Neville 1975).
33818824	13	175	theme	cells	2213:2217	arg1	Neville					2292:2298	Neville	2292:2298	Neville	2292:2298	A single layer of epithelial cells is responsible for the synthesis and secretion of both cuticular layers (Neville 1975).
33818824	22	176	theme	chitin	4115:4120	arg1	inhibition					4132:4141	chitin synthesis inhibition	4115:4141	chitin synthesis inhibition	4115:4141	In the case of chitin synthesis inhibition, molting disruption can be referred to as "premature molting."
33818824	5	177	theme	risk	865:868	arg1	assessment					870:879	risk assessment	865:879	risk assessment	865:879	The need for a more cost-efficient and mechanistic approach in risk assessment has been clearly evident and triggered the development of the adverse outcome pathway (AOP) framework (Ankley et al. 2010).
33818824	18	178	theme	synthase	3179:3186	arg1	isoform					3188:3194	chitin synthase isoform 1	3172:3196	chitin synthase isoform 1 (CHS-1)	3172:3204	Like the expression of chitin synthase isoform 1 (CHS-1), the expression of peptide hormones regulating the EMP is also controlled by ecdysteroids (Antoniewski et al. 1993; Gagou et al. 2002; Ayali 2009).
33818824	18	178	theme	synthase	3179:3186	arg1	CHS-1					3199:3203	CHS-1	3199:3203	CHS-1	3199:3203	Like the expression of chitin synthase isoform 1 (CHS-1), the expression of peptide hormones regulating the EMP is also controlled by ecdysteroids (Antoniewski et al. 1993; Gagou et al. 2002; Ayali 2009).
33818824	9	179	theme	biosynthetic	1646:1657	arg1	pathway					1659:1665	the chitin biosynthetic pathway	1635:1665	the chitin biosynthetic pathway (chitin synthesis inhibitors)	1635:1695	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	9	179	theme	biosynthetic	1646:1657	arg1	inhibitors					1685:1694	chitin synthesis inhibitors	1668:1694	chitin synthesis inhibitors	1668:1694	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	8	180	theme	AOP	1380:1382	arg1	framework					1384:1392	the AOP framework	1376:1392	the AOP framework	1376:1392	In the last decade, the AOP framework has gained traction and expanded within the (eco)toxicological research community.
33818824	6	181	theme	adverse	1084:1090	arg1	outcome					1092:1098	an adverse outcome	1081:1098	an adverse outcome	1081:1098	An AOP links a molecular initiating event (MIE) through key events (KEs) to an adverse outcome.
33818824	20	182	theme	different	3782:3790	arg1	isoforms					3796:3803	different CHS isoforms	3782:3803	different CHS isoforms	3782:3803	Because crustaceans are also dependent on the synthesis of chitin, the underlying mechanisms are believed to be similar, although less is known about different CHS isoforms and their localization (Rocha et al. 2012; Qian et al. 2014; Uddowla et al. 2014; Harðardóttir et al. 2019).
33818824	20	183	dep	localization	3815:3826	arg1	Rocha					3829:3833	Rocha	3829:3833	Rocha	3829:3833	Because crustaceans are also dependent on the synthesis of chitin, the underlying mechanisms are believed to be similar, although less is known about different CHS isoforms and their localization (Rocha et al. 2012; Qian et al. 2014; Uddowla et al. 2014; Harðardóttir et al. 2019).
33818824	29	184	theme	further	5293:5299	arg1	research					5301:5308	further research	5293:5308	further research	5293:5308	The development of this AOP will be useful in further research and regulatory initiatives related to assessment of CHS inhibitors and identification of critical knowledge gaps and may suggest new strategies for ecotoxicity testing efforts.
33818824	7	185	theme	processes	1163:1171	arg1	necessary					1225:1233	necessary	1225:1233	necessary	1225:1233	The mechanistic understanding of the underlying toxicological processes leading to a regulation-relevant adverse outcome is necessary for the utilization of new approach methodologies (NAMs) and efficient coverage of wider chemical and taxonomic domains.
33818824	7	185	theme	processes	1163:1171	arg1	understanding					1117:1129	The mechanistic understanding	1101:1129	The mechanistic understanding of the underlying toxicological processes leading to a regulation-relevant adverse outcome	1101:1220	The mechanistic understanding of the underlying toxicological processes leading to a regulation-relevant adverse outcome is necessary for the utilization of new approach methodologies (NAMs) and efficient coverage of wider chemical and taxonomic domains.
33818824	7	186	theme	mechanistic	1105:1115	arg1	necessary					1225:1233	necessary	1225:1233	necessary	1225:1233	The mechanistic understanding of the underlying toxicological processes leading to a regulation-relevant adverse outcome is necessary for the utilization of new approach methodologies (NAMs) and efficient coverage of wider chemical and taxonomic domains.
33818824	7	186	theme	mechanistic	1105:1115	arg1	understanding					1117:1129	The mechanistic understanding	1101:1129	The mechanistic understanding of the underlying toxicological processes leading to a regulation-relevant adverse outcome	1101:1220	The mechanistic understanding of the underlying toxicological processes leading to a regulation-relevant adverse outcome is necessary for the utilization of new approach methodologies (NAMs) and efficient coverage of wider chemical and taxonomic domains.
33818824	19	187	theme	plasma	3516:3521	arg1	membrane					3523:3530	the epithelial plasma membrane	3501:3530	the epithelial plasma membrane in insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006)	3501:3629	Cuticular chitin is polymerized from uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) by the transmembrane enzyme CHS-1, which is localized in the epithelial plasma membrane in insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006).
33818824	21	188	theme	synthesis	3942:3950	arg1	Disruption					3914:3923	Disruption	3914:3923	Disruption of either chitin synthesis or the upstream endocrine pathways	3914:3985	Disruption of either chitin synthesis or the upstream endocrine pathways can lead to lethal molting disruption (Arakawa et al. 2008; Merzendorfer et al. 2012; Song et al. 2017a, 2017b).
33818824	15	189	dep	crustaceans	2696:2706	arg1	Cribb					2709:2713	Cribb	2709:2713	Cribb	2709:2713	Because the procuticle mainly consists of chitin microfibrils embedded in a matrix of cuticular proteins supplemented by lipids and minerals in insects (Muthukrishnan et al. 2012) and crustaceans (Cribb et al. 2009; Nagasawa 2012), chitin is a determinant factor for the appropriate composition of the cuticle and successful molting (Cohen 2001).
33818824	18	190	theme	isoform	3188:3194	arg1	expression					3158:3167	the expression	3154:3167	the expression of chitin synthase isoform 1 (CHS-1)	3154:3204	Like the expression of chitin synthase isoform 1 (CHS-1), the expression of peptide hormones regulating the EMP is also controlled by ecdysteroids (Antoniewski et al. 1993; Gagou et al. 2002; Ayali 2009).
33818824	7	191	theme	new	1258:1260	arg1	NAMs					1286:1289	NAMs	1286:1289	NAMs	1286:1289	The mechanistic understanding of the underlying toxicological processes leading to a regulation-relevant adverse outcome is necessary for the utilization of new approach methodologies (NAMs) and efficient coverage of wider chemical and taxonomic domains.
33818824	7	191	theme	new	1258:1260	arg1	methodologies					1271:1283	new approach methodologies	1258:1283	new approach methodologies (NAMs)	1258:1290	The mechanistic understanding of the underlying toxicological processes leading to a regulation-relevant adverse outcome is necessary for the utilization of new approach methodologies (NAMs) and efficient coverage of wider chemical and taxonomic domains.
33818824	21	192	dep	Song	4073:4076	arg1	al.					4081:4083	Song et al.	4073:4083	Song et al. 2017a	4073:4089	Disruption of either chitin synthesis or the upstream endocrine pathways can lead to lethal molting disruption (Arakawa et al. 2008; Merzendorfer et al. 2012; Song et al. 2017a, 2017b).
33818824	21	192	dep	Song	4073:4076	arg1	2017a					4085:4089	2017a	4085:4089	Song et al. 2017a	4073:4089	Disruption of either chitin synthesis or the upstream endocrine pathways can lead to lethal molting disruption (Arakawa et al. 2008; Merzendorfer et al. 2012; Song et al. 2017a, 2017b).
33818824	15	193	dep	Cribb	2709:2713	arg1	Nagasawa					2728:2735	Nagasawa	2728:2735	Nagasawa	2728:2735	Because the procuticle mainly consists of chitin microfibrils embedded in a matrix of cuticular proteins supplemented by lipids and minerals in insects (Muthukrishnan et al. 2012) and crustaceans (Cribb et al. 2009; Nagasawa 2012), chitin is a determinant factor for the appropriate composition of the cuticle and successful molting (Cohen 2001).
33818824	29	194	theme	new	5439:5441	arg1	strategies					5443:5452	new strategies	5439:5452	new strategies for ecotoxicity testing efforts	5439:5484	The development of this AOP will be useful in further research and regulatory initiatives related to assessment of CHS inhibitors and identification of critical knowledge gaps and may suggest new strategies for ecotoxicity testing efforts.
33818824	9	195	theme	synthesis	1675:1683	arg1	pathway					1659:1665	the chitin biosynthetic pathway	1635:1665	the chitin biosynthetic pathway (chitin synthesis inhibitors)	1635:1695	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	9	195	theme	synthesis	1675:1683	arg1	inhibitors					1685:1694	chitin synthesis inhibitors	1668:1694	chitin synthesis inhibitors	1668:1694	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	12	196	theme	underlying	2151:2160	arg1	procuticle					2172:2181	the underlying chitinous procuticle	2147:2181	the underlying chitinous procuticle	2147:2181	The arthropod exoskeleton (cuticle) can be divided into 2 layers, the thin and nonchitinous epicuticle, which is the outermost layer of the cuticle, and the underlying chitinous procuticle.
33818824	17	197	theme	Song	3129:3132	arg1	2017a					3141:3145	Song et al. 2017a	3129:3145	EMP; Ayali 2009; Song et al. 2017a	3112:3145	The shedding of the old exoskeleton in insects is mediated by a sequence of distinct muscular contractions, the ecdysis motor program (EMP; Ayali 2009; Song et al. 2017a).
33818824	26	198	theme	incomplete	4840:4849	arg1	"					4859:4859	"	4859:4859	" which describes inhibition of ecdysis on a behavioral level	4859:4919	The term "premature molting" is used to differentiate from the term "incomplete ecdysis," which describes inhibition of ecdysis on a behavioral level, namely through reduction of the EMP (Song et al. 2017a).
33818824	26	198	theme	incomplete	4840:4849	arg1	ecdysis					4851:4857	the term "incomplete ecdysis	4830:4857	the term "incomplete ecdysis	4830:4857	The term "premature molting" is used to differentiate from the term "incomplete ecdysis," which describes inhibition of ecdysis on a behavioral level, namely through reduction of the EMP (Song et al. 2017a).
33818824	20	199	dep	Uddowla	3866:3872	arg1	2014					3881:3884	2014	3881:3884	2014	3881:3884	Because crustaceans are also dependent on the synthesis of chitin, the underlying mechanisms are believed to be similar, although less is known about different CHS isoforms and their localization (Rocha et al. 2012; Qian et al. 2014; Uddowla et al. 2014; Harðardóttir et al. 2019).
33818824	20	199	dep	Uddowla	3866:3872	arg1	al.					3877:3879	Uddowla et al.	3866:3879	Rocha et al. 2012; Qian et al. 2014; Uddowla et al. 2014; Harðardóttir et al. 2019	3829:3910	Because crustaceans are also dependent on the synthesis of chitin, the underlying mechanisms are believed to be similar, although less is known about different CHS isoforms and their localization (Rocha et al. 2012; Qian et al. 2014; Uddowla et al. 2014; Harðardóttir et al. 2019).
33818824	2	200	from	process	270:276	arg1	vertebrates					313:323	vertebrates	313:323	vertebrates	313:323	The highly conserved chitin synthetic process and the absence of this process in vertebrates make it an exploitable target for pest management and veterinary medicines (Merzendorfer 2013; Junquera et al. 2019).
33818824	9	201	from	inhibition	1601:1610	arg1	pathway					1659:1665	the chitin biosynthetic pathway	1635:1665	the chitin biosynthetic pathway (chitin synthesis inhibitors)	1635:1695	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	9	201	from	inhibition	1601:1610	arg1	inhibitors					1685:1694	chitin synthesis inhibitors	1668:1694	chitin synthesis inhibitors	1668:1694	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	22	202	theme	synthesis	4122:4130	arg1	inhibition					4132:4141	chitin synthesis inhibition	4115:4141	chitin synthesis inhibition	4115:4141	In the case of chitin synthesis inhibition, molting disruption can be referred to as "premature molting."
33818824	25	203	theme	intake	4725:4730	arg1	result					4687:4692	a result	4685:4692	a result of insufficient food or oxygen intake (Camp et al. 2014; Song et al. 2017a)	4685:4768	These effects are observed in arthropods following molting, which fail to survive subsequent molts (Arakawa et al. 2008; Chen et al. 2008) or animals being stuck in their exuviae (Wang et al. 2019) and ultimately dying as a result of insufficient food or oxygen intake (Camp et al. 2014; Song et al. 2017a).
33818824	24	204	theme	cuticle	4454:4460	arg1	integrity					4433:4441	the poor integrity	4424:4441	the poor integrity of the new cuticle	4424:4460	Even if ecdysis can be completed on inhibition of chitin synthesis, the organism may not survive because of the poor integrity of the new cuticle.
33818824	17	205	theme	distinct	3053:3060	arg1	contractions					3071:3082	distinct muscular contractions	3053:3082	distinct muscular contractions	3053:3082	The shedding of the old exoskeleton in insects is mediated by a sequence of distinct muscular contractions, the ecdysis motor program (EMP; Ayali 2009; Song et al. 2017a).
33818824	17	205	theme	distinct	3053:3060	arg1	program					3103:3109	the ecdysis motor program	3085:3109	the ecdysis motor program (EMP; Ayali 2009; Song et al. 2017a)	3085:3146	The shedding of the old exoskeleton in insects is mediated by a sequence of distinct muscular contractions, the ecdysis motor program (EMP; Ayali 2009; Song et al. 2017a).
33818824	17	206	theme	exoskeleton	3001:3011	arg1	shedding					2981:2988	The shedding	2977:2988	The shedding of the old exoskeleton in insects	2977:3022	The shedding of the old exoskeleton in insects is mediated by a sequence of distinct muscular contractions, the ecdysis motor program (EMP; Ayali 2009; Song et al. 2017a).
33818824	2	207	dep	medicines	390:398	arg1	Merzendorfer					401:412	Merzendorfer	401:412	Merzendorfer	401:412	The highly conserved chitin synthetic process and the absence of this process in vertebrates make it an exploitable target for pest management and veterinary medicines (Merzendorfer 2013; Junquera et al. 2019).
33818824	18	208	dep	Antoniewski	3297:3307	arg1	Ayali					3341:3345	Ayali	3341:3345	Ayali	3341:3345	Like the expression of chitin synthase isoform 1 (CHS-1), the expression of peptide hormones regulating the EMP is also controlled by ecdysteroids (Antoniewski et al. 1993; Gagou et al. 2002; Ayali 2009).
33818824	18	208	dep	Antoniewski	3297:3307	arg1	Gagou					3322:3326	Gagou	3322:3326	Gagou	3322:3326	Like the expression of chitin synthase isoform 1 (CHS-1), the expression of peptide hormones regulating the EMP is also controlled by ecdysteroids (Antoniewski et al. 1993; Gagou et al. 2002; Ayali 2009).
33818824	18	208	dep	Antoniewski	3297:3307	arg1	al.					3312:3314	Antoniewski et al.	3297:3314	Antoniewski et al. 1993; Gagou et al. 2002; Ayali 2009	3297:3350	Like the expression of chitin synthase isoform 1 (CHS-1), the expression of peptide hormones regulating the EMP is also controlled by ecdysteroids (Antoniewski et al. 1993; Gagou et al. 2002; Ayali 2009).
33818824	18	208	dep	Antoniewski	3297:3307	arg1	1993					3316:3319	1993	3316:3319	1993	3316:3319	Like the expression of chitin synthase isoform 1 (CHS-1), the expression of peptide hormones regulating the EMP is also controlled by ecdysteroids (Antoniewski et al. 1993; Gagou et al. 2002; Ayali 2009).
33818824	15	209	theme	cuticle	2814:2820	arg1	Cohen					2846:2850	Cohen	2846:2850	Cohen	2846:2850	Because the procuticle mainly consists of chitin microfibrils embedded in a matrix of cuticular proteins supplemented by lipids and minerals in insects (Muthukrishnan et al. 2012) and crustaceans (Cribb et al. 2009; Nagasawa 2012), chitin is a determinant factor for the appropriate composition of the cuticle and successful molting (Cohen 2001).
33818824	15	209	theme	cuticle	2814:2820	arg1	composition					2795:2805	the appropriate composition	2779:2805	the appropriate composition of the cuticle	2779:2820	Because the procuticle mainly consists of chitin microfibrils embedded in a matrix of cuticular proteins supplemented by lipids and minerals in insects (Muthukrishnan et al. 2012) and crustaceans (Cribb et al. 2009; Nagasawa 2012), chitin is a determinant factor for the appropriate composition of the cuticle and successful molting (Cohen 2001).
33818824	15	209	theme	cuticle	2814:2820	arg1	molting					2837:2843	successful molting	2826:2843	successful molting (Cohen 2001)	2826:2856	Because the procuticle mainly consists of chitin microfibrils embedded in a matrix of cuticular proteins supplemented by lipids and minerals in insects (Muthukrishnan et al. 2012) and crustaceans (Cribb et al. 2009; Nagasawa 2012), chitin is a determinant factor for the appropriate composition of the cuticle and successful molting (Cohen 2001).
33818824	26	210	theme	term	4834:4837	arg1	"					4859:4859	"	4859:4859	" which describes inhibition of ecdysis on a behavioral level	4859:4919	The term "premature molting" is used to differentiate from the term "incomplete ecdysis," which describes inhibition of ecdysis on a behavioral level, namely through reduction of the EMP (Song et al. 2017a).
33818824	26	210	theme	term	4834:4837	arg1	ecdysis					4851:4857	the term "incomplete ecdysis	4830:4857	the term "incomplete ecdysis	4830:4857	The term "premature molting" is used to differentiate from the term "incomplete ecdysis," which describes inhibition of ecdysis on a behavioral level, namely through reduction of the EMP (Song et al. 2017a).
33818824	12	211	theme	cuticle	2134:2140	arg1	epicuticle					2086:2095	the thin and nonchitinous epicuticle	2060:2095	the thin and nonchitinous epicuticle	2060:2095	The arthropod exoskeleton (cuticle) can be divided into 2 layers, the thin and nonchitinous epicuticle, which is the outermost layer of the cuticle, and the underlying chitinous procuticle.
33818824	12	211	theme	cuticle	2134:2140	arg1	layer					2121:2125	the outermost layer	2107:2125	the outermost layer of the cuticle	2107:2140	The arthropod exoskeleton (cuticle) can be divided into 2 layers, the thin and nonchitinous epicuticle, which is the outermost layer of the cuticle, and the underlying chitinous procuticle.
33818824	12	211	theme	cuticle	2134:2140	arg1	layers					2052:2057	2 layers	2050:2057	2 layers	2050:2057	The arthropod exoskeleton (cuticle) can be divided into 2 layers, the thin and nonchitinous epicuticle, which is the outermost layer of the cuticle, and the underlying chitinous procuticle.
33818824	17	212	dep	Song	3129:3132	arg1	al.					3137:3139	Song et al.	3129:3139	EMP; Ayali 2009; Song et al. 2017a	3112:3145	The shedding of the old exoskeleton in insects is mediated by a sequence of distinct muscular contractions, the ecdysis motor program (EMP; Ayali 2009; Song et al. 2017a).
33818824	17	213	theme	contractions	3071:3082	arg1	sequence					3041:3048	a sequence	3039:3048	a sequence of distinct muscular contractions, the ecdysis motor program (EMP; Ayali 2009; Song et al. 2017a)	3039:3146	The shedding of the old exoskeleton in insects is mediated by a sequence of distinct muscular contractions, the ecdysis motor program (EMP; Ayali 2009; Song et al. 2017a).
33818824	2	214	theme	exploitable	336:346	arg1	target					348:353	an exploitable target	333:353	it an exploitable target for pest management and veterinary medicines (Merzendorfer 2013; Junquera et al. 2019)	330:440	The highly conserved chitin synthetic process and the absence of this process in vertebrates make it an exploitable target for pest management and veterinary medicines (Merzendorfer 2013; Junquera et al. 2019).
33818824	2	215	dep	Merzendorfer	401:412	arg1	Junquera					420:427	Junquera	420:427	Junquera	420:427	The highly conserved chitin synthetic process and the absence of this process in vertebrates make it an exploitable target for pest management and veterinary medicines (Merzendorfer 2013; Junquera et al. 2019).
33818824	2	215	dep	Merzendorfer	401:412	arg1	2013					414:417	2013	414:417	Merzendorfer 2013; Junquera et al. 2019	401:439	The highly conserved chitin synthetic process and the absence of this process in vertebrates make it an exploitable target for pest management and veterinary medicines (Merzendorfer 2013; Junquera et al. 2019).
33818824	29	216	theme	critical	5399:5406	arg1	gaps					5418:5421	critical knowledge gaps	5399:5421	critical knowledge gaps	5399:5421	The development of this AOP will be useful in further research and regulatory initiatives related to assessment of CHS inhibitors and identification of critical knowledge gaps and may suggest new strategies for ecotoxicity testing efforts.
33818824	3	217	dep	Susceptible	443:453	arg1	nontarget					456:464	nontarget	456:464	nontarget	456:464	Susceptible, nontarget organisms, such as insects and aquatic invertebrates, exposed to chitin synthesis inhibitors may suffer population declines, which may have a negative impact on ecosystems and associated services.
33818824	9	218	theme	associated	1718:1727	arg1	systems					1739:1745	associated endocrine systems	1718:1745	associated endocrine systems	1718:1745	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	8	219	theme	eco	1439:1441	arg1	community					1466:1474	the (eco)toxicological research community	1434:1474	the (eco)toxicological research community	1434:1474	In the last decade, the AOP framework has gained traction and expanded within the (eco)toxicological research community.
33818824	20	220	theme	underlying	3703:3712	arg1	similar					3744:3750	similar	3744:3750	similar	3744:3750	Because crustaceans are also dependent on the synthesis of chitin, the underlying mechanisms are believed to be similar, although less is known about different CHS isoforms and their localization (Rocha et al. 2012; Qian et al. 2014; Uddowla et al. 2014; Harðardóttir et al. 2019).
33818824	20	220	theme	underlying	3703:3712	arg1	mechanisms					3714:3723	the underlying mechanisms	3699:3723	the underlying mechanisms	3699:3723	Because crustaceans are also dependent on the synthesis of chitin, the underlying mechanisms are believed to be similar, although less is known about different CHS isoforms and their localization (Rocha et al. 2012; Qian et al. 2014; Uddowla et al. 2014; Harðardóttir et al. 2019).
33818824	9	221	theme	mature	1509:1514	arg1	AOPs					1529:1532	mature invertebrate AOPs	1509:1532	mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors)	1509:1795	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	18	222	dep	controlled	3269:3278	arg1	Antoniewski					3297:3307	Antoniewski	3297:3307	Antoniewski	3297:3307	Like the expression of chitin synthase isoform 1 (CHS-1), the expression of peptide hormones regulating the EMP is also controlled by ecdysteroids (Antoniewski et al. 1993; Gagou et al. 2002; Ayali 2009).
33818824	13	223	theme	cuticular	2274:2282	arg1	layers					2284:2289	both cuticular layers	2269:2289	both cuticular layers	2269:2289	A single layer of epithelial cells is responsible for the synthesis and secretion of both cuticular layers (Neville 1975).
33818824	22	224	dep	referred	4170:4177	arg1	"					4204:4204	"	4204:4204	"	4204:4204	In the case of chitin synthesis inhibition, molting disruption can be referred to as "premature molting."
33818824	15	225	theme	appropriate	2783:2793	arg1	composition					2795:2805	the appropriate composition	2779:2805	the appropriate composition of the cuticle	2779:2820	Because the procuticle mainly consists of chitin microfibrils embedded in a matrix of cuticular proteins supplemented by lipids and minerals in insects (Muthukrishnan et al. 2012) and crustaceans (Cribb et al. 2009; Nagasawa 2012), chitin is a determinant factor for the appropriate composition of the cuticle and successful molting (Cohen 2001).
33818824	2	226	theme	veterinary	379:388	arg1	medicines					390:398	veterinary medicines	379:398	veterinary medicines (Merzendorfer 2013; Junquera et al. 2019)	379:440	The highly conserved chitin synthetic process and the absence of this process in vertebrates make it an exploitable target for pest management and veterinary medicines (Merzendorfer 2013; Junquera et al. 2019).
33818824	8	227	theme	toxicological	1443:1455	arg1	community					1466:1474	the (eco)toxicological research community	1434:1474	the (eco)toxicological research community	1434:1474	In the last decade, the AOP framework has gained traction and expanded within the (eco)toxicological research community.
33818824	29	228	theme	ecotoxicity	5458:5468	arg1	efforts					5478:5484	ecotoxicity testing efforts	5458:5484	ecotoxicity testing efforts	5458:5484	The development of this AOP will be useful in further research and regulatory initiatives related to assessment of CHS inhibitors and identification of critical knowledge gaps and may suggest new strategies for ecotoxicity testing efforts.
33818824	6	229	attach	links	1012:1016	arg1	outcome					1092:1098	an adverse outcome	1081:1098	an adverse outcome	1081:1098	An AOP links a molecular initiating event (MIE) through key events (KEs) to an adverse outcome.
33818824	6	229	attach	links	1012:1016	arg2	AOP					1008:1010	An AOP	1005:1010	An AOP	1005:1010	An AOP links a molecular initiating event (MIE) through key events (KEs) to an adverse outcome.
33818824	24	230	theme	new	4450:4452	arg1	cuticle					4454:4460	the new cuticle	4446:4460	the new cuticle	4446:4460	Even if ecdysis can be completed on inhibition of chitin synthesis, the organism may not survive because of the poor integrity of the new cuticle.
33818824	17	231	theme	motor	3097:3101	arg1	contractions					3071:3082	distinct muscular contractions	3053:3082	distinct muscular contractions	3053:3082	The shedding of the old exoskeleton in insects is mediated by a sequence of distinct muscular contractions, the ecdysis motor program (EMP; Ayali 2009; Song et al. 2017a).
33818824	17	231	theme	motor	3097:3101	arg1	program					3103:3109	the ecdysis motor program	3085:3109	the ecdysis motor program (EMP; Ayali 2009; Song et al. 2017a)	3085:3146	The shedding of the old exoskeleton in insects is mediated by a sequence of distinct muscular contractions, the ecdysis motor program (EMP; Ayali 2009; Song et al. 2017a).
33818824	25	232	dep	food	4710:4713	arg1	Camp					4733:4736	Camp	4733:4736	Camp et al. 2014; Song et al. 2017a	4733:4767	These effects are observed in arthropods following molting, which fail to survive subsequent molts (Arakawa et al. 2008; Chen et al. 2008) or animals being stuck in their exuviae (Wang et al. 2019) and ultimately dying as a result of insufficient food or oxygen intake (Camp et al. 2014; Song et al. 2017a).
33818824	0	233	theme	Chitin	26:31	arg1	Synthase					33:40	Chitin Synthase 1	26:42	Chitin Synthase 1	26:42	AOP Report: Inhibition of Chitin Synthase 1 Leading to Increased Mortality in Arthropods.
33818824	9	234	from	interference	1700:1711	arg1	pathway					1659:1665	the chitin biosynthetic pathway	1635:1665	the chitin biosynthetic pathway (chitin synthesis inhibitors)	1635:1695	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	9	234	from	interference	1700:1711	arg1	inhibitors					1685:1694	chitin synthesis inhibitors	1668:1694	chitin synthesis inhibitors	1668:1694	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	5	235	theme	pathway	959:965	arg1	framework					973:981	pathway (AOP) framework	959:981	the adverse outcome pathway (AOP) framework (Ankley et al. 2010)	939:1002	The need for a more cost-efficient and mechanistic approach in risk assessment has been clearly evident and triggered the development of the adverse outcome pathway (AOP) framework (Ankley et al. 2010).
33818824	7	236	theme	regulation-relevant	1186:1204	arg1	outcome					1214:1220	a regulation-relevant adverse outcome	1184:1220	a regulation-relevant adverse outcome	1184:1220	The mechanistic understanding of the underlying toxicological processes leading to a regulation-relevant adverse outcome is necessary for the utilization of new approach methodologies (NAMs) and efficient coverage of wider chemical and taxonomic domains.
33818824	9	237	theme	environmental	1750:1762	arg1	chemicals					1764:1772	environmental chemicals	1750:1772	environmental chemicals (endocrine disruptors)	1750:1795	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	9	237	theme	environmental	1750:1762	arg1	disruptors					1785:1794	endocrine disruptors	1775:1794	endocrine disruptors	1775:1794	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	15	238	from	lipids	2633:2638	arg1	insects					2656:2662	insects	2656:2662	insects (Muthukrishnan et al. 2012)	2656:2690	Because the procuticle mainly consists of chitin microfibrils embedded in a matrix of cuticular proteins supplemented by lipids and minerals in insects (Muthukrishnan et al. 2012) and crustaceans (Cribb et al. 2009; Nagasawa 2012), chitin is a determinant factor for the appropriate composition of the cuticle and successful molting (Cohen 2001).
33818824	15	238	from	lipids	2633:2638	arg1	crustaceans					2696:2706	crustaceans	2696:2706	crustaceans (Cribb et al. 2009; Nagasawa 2012)	2696:2741	Because the procuticle mainly consists of chitin microfibrils embedded in a matrix of cuticular proteins supplemented by lipids and minerals in insects (Muthukrishnan et al. 2012) and crustaceans (Cribb et al. 2009; Nagasawa 2012), chitin is a determinant factor for the appropriate composition of the cuticle and successful molting (Cohen 2001).
33818824	29	239	theme	regulatory	5314:5323	arg1	initiatives					5325:5335	regulatory initiatives	5314:5335	regulatory initiatives related to assessment of CHS inhibitors and identification of critical knowledge gaps	5314:5421	The development of this AOP will be useful in further research and regulatory initiatives related to assessment of CHS inhibitors and identification of critical knowledge gaps and may suggest new strategies for ecotoxicity testing efforts.
33818824	29	240	theme	knowledge	5408:5416	arg1	gaps					5418:5421	critical knowledge gaps	5399:5421	critical knowledge gaps	5399:5421	The development of this AOP will be useful in further research and regulatory initiatives related to assessment of CHS inhibitors and identification of critical knowledge gaps and may suggest new strategies for ecotoxicity testing efforts.
33818824	5	241	theme	AOP	968:970	arg1	framework					973:981	pathway (AOP) framework	959:981	the adverse outcome pathway (AOP) framework (Ankley et al. 2010)	939:1002	The need for a more cost-efficient and mechanistic approach in risk assessment has been clearly evident and triggered the development of the adverse outcome pathway (AOP) framework (Ankley et al. 2010).
33818824	15	242	from	matrix	2588:2593	arg1	embedded					2574:2581	embedded	2574:2581	embedded	2574:2581	Because the procuticle mainly consists of chitin microfibrils embedded in a matrix of cuticular proteins supplemented by lipids and minerals in insects (Muthukrishnan et al. 2012) and crustaceans (Cribb et al. 2009; Nagasawa 2012), chitin is a determinant factor for the appropriate composition of the cuticle and successful molting (Cohen 2001).
33818824	25	243	dep	exuviae	4634:4640	arg1	Wang					4643:4646	Wang	4643:4646	Wang	4643:4646	These effects are observed in arthropods following molting, which fail to survive subsequent molts (Arakawa et al. 2008; Chen et al. 2008) or animals being stuck in their exuviae (Wang et al. 2019) and ultimately dying as a result of insufficient food or oxygen intake (Camp et al. 2014; Song et al. 2017a).
33818824	15	244	from	minerals	2644:2651	arg1	insects					2656:2662	insects	2656:2662	insects (Muthukrishnan et al. 2012)	2656:2690	Because the procuticle mainly consists of chitin microfibrils embedded in a matrix of cuticular proteins supplemented by lipids and minerals in insects (Muthukrishnan et al. 2012) and crustaceans (Cribb et al. 2009; Nagasawa 2012), chitin is a determinant factor for the appropriate composition of the cuticle and successful molting (Cohen 2001).
33818824	15	244	from	minerals	2644:2651	arg1	crustaceans					2696:2706	crustaceans	2696:2706	crustaceans (Cribb et al. 2009; Nagasawa 2012)	2696:2741	Because the procuticle mainly consists of chitin microfibrils embedded in a matrix of cuticular proteins supplemented by lipids and minerals in insects (Muthukrishnan et al. 2012) and crustaceans (Cribb et al. 2009; Nagasawa 2012), chitin is a determinant factor for the appropriate composition of the cuticle and successful molting (Cohen 2001).
33818824	3	245	theme	negative	608:615	arg1	impact					617:622	a negative impact	606:622	a negative impact	606:622	Susceptible, nontarget organisms, such as insects and aquatic invertebrates, exposed to chitin synthesis inhibitors may suffer population declines, which may have a negative impact on ecosystems and associated services.
33818824	4	246	theme	potential	759:767	arg1	hazards					769:775	potential hazards	759:775	potential hazards	759:775	Hence, it is important to properly identify, prioritize, and regulate relevant chemicals posing potential hazards to nontarget arthropods.
33818824	2	247	theme	chitin	253:258	arg1	process					270:276	The highly conserved chitin synthetic process	232:276	The highly conserved chitin synthetic process	232:276	The highly conserved chitin synthetic process and the absence of this process in vertebrates make it an exploitable target for pest management and veterinary medicines (Merzendorfer 2013; Junquera et al. 2019).
33818824	5	248	theme	outcome	951:957	arg1	development					924:934	the development	920:934	the development of the adverse outcome pathway (AOP) framework (Ankley et al. 2010)	920:1002	The need for a more cost-efficient and mechanistic approach in risk assessment has been clearly evident and triggered the development of the adverse outcome pathway (AOP) framework (Ankley et al. 2010).
33818824	25	249	dep	Chen	4584:4587	arg1	al.					4592:4594	Chen et al.	4584:4594	Chen et al. 2008	4584:4599	These effects are observed in arthropods following molting, which fail to survive subsequent molts (Arakawa et al. 2008; Chen et al. 2008) or animals being stuck in their exuviae (Wang et al. 2019) and ultimately dying as a result of insufficient food or oxygen intake (Camp et al. 2014; Song et al. 2017a).
33818824	25	249	dep	Chen	4584:4587	arg1	2008					4596:4599	2008	4596:4599	2008	4596:4599	These effects are observed in arthropods following molting, which fail to survive subsequent molts (Arakawa et al. 2008; Chen et al. 2008) or animals being stuck in their exuviae (Wang et al. 2019) and ultimately dying as a result of insufficient food or oxygen intake (Camp et al. 2014; Song et al. 2017a).
33818824	0	250	dep	Inhibition	12:21	arg1	Report					4:9	AOP Report	0:9	AOP Report	0:9	AOP Report: Inhibition of Chitin Synthase 1 Leading to Increased Mortality in Arthropods.
33818824	9	251	with	interference	1700:1711	arg1	systems					1739:1745	associated endocrine systems	1718:1745	associated endocrine systems	1718:1745	However, there exists a lack of mature invertebrate AOPs describing molting defect-associated mortality triggered by direct inhibition of relevant enzymes in the chitin biosynthetic pathway (chitin synthesis inhibitors) or interference with associated endocrine systems by environmental chemicals (endocrine disruptors).
33818824	6	252	theme	initiating	1030:1039	arg1	MIE					1048:1050	MIE	1048:1050	MIE	1048:1050	An AOP links a molecular initiating event (MIE) through key events (KEs) to an adverse outcome.
33818824	6	252	theme	initiating	1030:1039	arg1	event					1041:1045	a molecular initiating event	1018:1045	a molecular initiating event (MIE)	1018:1051	An AOP links a molecular initiating event (MIE) through key events (KEs) to an adverse outcome.
33818824	28	253	theme	increasing	5106:5115	arg1	number					5117:5122	the small but increasing number	5092:5122	the small but increasing number of invertebrate AOPs that have relevance to arthropods, the largest phylum within the animal kingdom (Bar-On et al. 2018)	5092:5244	It expands the small but increasing number of invertebrate AOPs that have relevance to arthropods, the largest phylum within the animal kingdom (Bar-On et al. 2018).
33818824	10	254	dep	undergo	1809:1815	arg1	Heming					1848:1853	Heming	1848:1853	Heming	1848:1853	Arthropods undergo molting to grow and reproduce (Heming 2018).
33818824	27	255	theme	CHS-1	5074:5078	arg1	inhibition					5049:5058	direct inhibition	5042:5058	direct inhibition of the enzyme CHS-1	5042:5078	The present AOP describes molting-associated mortality through direct inhibition of the enzyme CHS-1.
33818824	19	256	from	diphosphate-N-acetylglucosamine	3399:3429	arg1	polymerized					3374:3384	polymerized	3374:3384	polymerized	3374:3384	Cuticular chitin is polymerized from uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) by the transmembrane enzyme CHS-1, which is localized in the epithelial plasma membrane in insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006).
33818824	2	257	dep	it	330:331	arg1	target					348:353	an exploitable target	333:353	it an exploitable target for pest management and veterinary medicines (Merzendorfer 2013; Junquera et al. 2019)	330:440	The highly conserved chitin synthetic process and the absence of this process in vertebrates make it an exploitable target for pest management and veterinary medicines (Merzendorfer 2013; Junquera et al. 2019).
33818824	32	258	theme	Environmental	5547:5559	arg1	Toxicology					5561:5570	Environmental Toxicology	5547:5570	Environmental Toxicology	5547:5570	Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
33818824	5	259	theme	adverse	943:949	arg1	outcome					951:957	the adverse outcome pathway (AOP) framework (Ankley et al. 2010)	939:1002	the adverse outcome pathway (AOP) framework (Ankley et al. 2010)	939:1002	The need for a more cost-efficient and mechanistic approach in risk assessment has been clearly evident and triggered the development of the adverse outcome pathway (AOP) framework (Ankley et al. 2010).
33818824	7	260	theme	underlying	1138:1147	arg1	processes					1163:1171	the underlying toxicological processes	1134:1171	the underlying toxicological processes leading to a regulation-relevant adverse outcome	1134:1220	The mechanistic understanding of the underlying toxicological processes leading to a regulation-relevant adverse outcome is necessary for the utilization of new approach methodologies (NAMs) and efficient coverage of wider chemical and taxonomic domains.
33818824	19	261	dep	Huie	3554:3557	arg1	Merzendorfer					3612:3623	Merzendorfer	3612:3623	Merzendorfer	3612:3623	Cuticular chitin is polymerized from uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) by the transmembrane enzyme CHS-1, which is localized in the epithelial plasma membrane in insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006).
33818824	19	261	dep	Huie	3554:3557	arg1	2006					3625:3628	2006	3625:3628	Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006	3554:3628	Cuticular chitin is polymerized from uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) by the transmembrane enzyme CHS-1, which is localized in the epithelial plasma membrane in insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006).
33818824	19	261	dep	Huie	3554:3557	arg1	Zimoch					3599:3604	Zimoch	3599:3604	Zimoch	3599:3604	Cuticular chitin is polymerized from uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) by the transmembrane enzyme CHS-1, which is localized in the epithelial plasma membrane in insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006).
33818824	19	261	dep	Huie	3554:3557	arg1	Binnington					3565:3574	Binnington	3565:3574	Binnington	3565:3574	Cuticular chitin is polymerized from uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) by the transmembrane enzyme CHS-1, which is localized in the epithelial plasma membrane in insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006).
33818824	19	261	dep	Huie	3554:3557	arg1	Merzendorfer					3582:3593	Merzendorfer	3582:3593	Merzendorfer	3582:3593	Cuticular chitin is polymerized from uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) by the transmembrane enzyme CHS-1, which is localized in the epithelial plasma membrane in insects (Locke and Huie 1979; Binnington 1985; Merzendorfer and Zimoch 2003; Merzendorfer 2006).
34482828	8	0	theme	34-36 kDa	1571:1579	arg1	ranges					1543:1548	the ranges	1539:1548	the ranges of 40-45, 37-42, and 34-36 kDa, respectively	1539:1593	The molecular weight of rec-eCGβ/α, rec-eCGβ/αΔ56 and rec-eCG β-D/α were in the ranges of 40-45, 37-42, and 34-36 kDa, respectively.
34482828	0	1	theme	hormone	201:207	arg1	receptor					209:216	follicle-stimulating hormone receptor	180:216	follicle-stimulating hormone receptor	180:216	Roles of N-linked and O-linked glycosylation sites in the activity of equine chorionic gonadotropin in cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating hormone receptor.
34482828	9	2	with	Treatment	1596:1604	arg1	F					1631:1631	peptide-N-glycanase F	1611:1631	peptide-N-glycanase F	1611:1631	Treatment with peptide-N-glycanase F markedly decreased the molecular weight to approximately 5-10 kDa.
34482828	5	3	theme	CHO-S	1174:1178	arg1	medium					1193:1198	the CHO-S cell culture medium	1170:1198	the CHO-S cell culture medium	1170:1198	RESULTS Both rec-eCGβ/α and rec-eCGβ/αΔ56 were efficiently secreted into the CHO-S cell culture medium on day 1 post-transfection.
34482828	8	4	theme	molecular	1467:1475	arg1	weight					1477:1482	The molecular weight	1463:1482	The molecular weight of rec-eCGβ/α, rec-eCGβ/αΔ56 and rec-eCG β-D/α	1463:1529	The molecular weight of rec-eCGβ/α, rec-eCGβ/αΔ56 and rec-eCG β-D/α were in the ranges of 40-45, 37-42, and 34-36 kDa, respectively.
34482828	0	5	link	N-linked	9:16	arg1	sites					45:49	N-linked and O-linked glycosylation sites	9:49	N-linked and O-linked glycosylation sites	9:49	Roles of N-linked and O-linked glycosylation sites in the activity of equine chorionic gonadotropin in cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating hormone receptor.
34482828	4	6	theme	lutropin/CG	1036:1046	arg1	rLH/CGR					1058:1064	rLH/CGR	1058:1064	rLH/CGR	1058:1064	The FSH-like and LH-like activities of the mutants were examined using CHO-K1 cells expressing rat lutropin/CG receptor (rLH/CGR) and rat FSH receptor (rFSHR).
34482828	4	6	theme	lutropin/CG	1036:1046	arg1	receptor					1048:1055	rat lutropin/CG receptor	1032:1055	rat lutropin/CG receptor (rLH/CGR)	1032:1065	The FSH-like and LH-like activities of the mutants were examined using CHO-K1 cells expressing rat lutropin/CG receptor (rLH/CGR) and rat FSH receptor (rFSHR).
34482828	15	7	from	activity	2169:2176	arg1	cells					2181:2185	cells	2181:2185	cells expressing rLH/CGR and rFSHR	2181:2214	CONCLUSIONS Rec-eCGβ/α exhibits potent biological activity in cells expressing rLH/CGR and rFSHR.
34482828	16	8	theme	α-subunit	2369:2377	arg1	Asn56					2352:2356	Asn56	2352:2356	Asn56 of the eCG α-subunit	2352:2377	The findings of this study suggest that the LH-like and FSH-like activities of eCG are regulated by the N-linked glycosylation site at Asn56 of the eCG α-subunit and/or by the O-linked glycosylation sites of the eCG β-subunit.
34482828	10	9	theme	LH-like	1747:1753	arg1	activity					1755:1762	downregulated LH-like activity	1733:1762	downregulated LH-like activity	1733:1762	Rec-eCGβ/αΔ56 exhibited markedly downregulated LH-like activity.
34482828	1	10	gly	glycoprotein	354:365	arg1	glycoprotein					354:365	the glycoprotein hormone family	350:380	the glycoprotein hormone family	350:380	BACKGROUND Equine chorionic gonadotropin (eCG), which comprises highly glycosylated α-subunit and β-subunit, is a unique member of the glycoprotein hormone family as it elicits both follicle-stimulating hormone (FSH)-like and luteinizing hormone (LH)-like responses in non-equid species.
34482828	5	11	theme	culture	1185:1191	arg1	medium					1193:1198	the CHO-S cell culture medium	1170:1198	the CHO-S cell culture medium	1170:1198	RESULTS Both rec-eCGβ/α and rec-eCGβ/αΔ56 were efficiently secreted into the CHO-S cell culture medium on day 1 post-transfection.
34482828	7	12	theme	days	1432:1435	arg1	post-transfection					1444:1460	days 3 to 7 post-transfection	1432:1460	days 3 to 7 post-transfection	1432:1460	The expression levels of all mutants were similar (200-250 mIU/mL) from days 3 to 7 post-transfection.
34482828	7	13	theme	mutants	1389:1395	arg1	200-250 mIU/mL					1411:1424	200-250 mIU/mL	1411:1424	200-250 mIU/mL	1411:1424	The expression levels of all mutants were similar (200-250 mIU/mL) from days 3 to 7 post-transfection.
34482828	7	13	theme	mutants	1389:1395	arg1	similar					1402:1408	similar	1402:1408	similar	1402:1408	The expression levels of all mutants were similar (200-250 mIU/mL) from days 3 to 7 post-transfection.
34482828	7	13	theme	mutants	1389:1395	arg1	levels					1375:1380	The expression levels	1360:1380	The expression levels of all mutants	1360:1395	The expression levels of all mutants were similar (200-250 mIU/mL) from days 3 to 7 post-transfection.
34482828	2	14	theme	peptide	766:772	arg1	region					780:785	the carboxyl-terminal peptide (CTP) region	744:785	the carboxyl-terminal peptide (CTP) region of the β-subunit	744:802	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	2	15	from	deletion	697:704	arg1	region					780:785	the carboxyl-terminal peptide (CTP) region	744:785	the carboxyl-terminal peptide (CTP) region of the β-subunit	744:802	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	7	16	from	post-transfection	1444:1460	arg1	200-250 mIU/mL					1411:1424	200-250 mIU/mL	1411:1424	200-250 mIU/mL	1411:1424	The expression levels of all mutants were similar (200-250 mIU/mL) from days 3 to 7 post-transfection.
34482828	7	16	from	post-transfection	1444:1460	arg1	similar					1402:1408	similar	1402:1408	similar	1402:1408	The expression levels of all mutants were similar (200-250 mIU/mL) from days 3 to 7 post-transfection.
34482828	7	16	from	post-transfection	1444:1460	arg1	levels					1375:1380	The expression levels	1360:1380	The expression levels of all mutants	1360:1395	The expression levels of all mutants were similar (200-250 mIU/mL) from days 3 to 7 post-transfection.
34482828	1	17	theme	-like	469:473	arg1	responses					475:483	both follicle-stimulating hormone (FSH)-like and luteinizing hormone (LH)-like responses	396:483	both follicle-stimulating hormone (FSH)-like and luteinizing hormone (LH)-like responses	396:483	BACKGROUND Equine chorionic gonadotropin (eCG), which comprises highly glycosylated α-subunit and β-subunit, is a unique member of the glycoprotein hormone family as it elicits both follicle-stimulating hormone (FSH)-like and luteinizing hormone (LH)-like responses in non-equid species.
34482828	2	18	dep	eCGβ/αΔ56	631:639	arg1	eCGβ-D/α					687:694	eCGβ-D/α	687:694	eCGβ-D/α	687:694	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	2	18	dep	eCGβ/αΔ56	631:639	arg1	mutant					825:830	double mutant	818:830	double mutant	818:830	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	2	18	dep	eCGβ/αΔ56	631:639	arg1	deletion					697:704	deletion	697:704	deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit	697:802	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	2	18	dep	eCGβ/αΔ56	631:639	arg1	substitution					642:653	substitution	642:653	substitution of Asn56 of α-subunit with Gln	642:684	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	16	19	theme	O-linked	2393:2400	arg1	β-subunit					2433:2441	the eCG β-subunit	2425:2441	the eCG β-subunit	2425:2441	The findings of this study suggest that the LH-like and FSH-like activities of eCG are regulated by the N-linked glycosylation site at Asn56 of the eCG α-subunit and/or by the O-linked glycosylation sites of the eCG β-subunit.
34482828	16	19	theme	O-linked	2393:2400	arg1	sites					2416:2420	the O-linked glycosylation sites	2389:2420	the O-linked glycosylation sites of the eCG β-subunit	2389:2441	The findings of this study suggest that the LH-like and FSH-like activities of eCG are regulated by the N-linked glycosylation site at Asn56 of the eCG α-subunit and/or by the O-linked glycosylation sites of the eCG β-subunit.
34482828	1	20	theme	BACKGROUND	219:228	arg1	member					340:345	a unique member	331:345	a unique member of the glycoprotein hormone family	331:380	BACKGROUND Equine chorionic gonadotropin (eCG), which comprises highly glycosylated α-subunit and β-subunit, is a unique member of the glycoprotein hormone family as it elicits both follicle-stimulating hormone (FSH)-like and luteinizing hormone (LH)-like responses in non-equid species.
34482828	1	20	theme	BACKGROUND	219:228	arg1	eCG					261:263	eCG	261:263	eCG	261:263	BACKGROUND Equine chorionic gonadotropin (eCG), which comprises highly glycosylated α-subunit and β-subunit, is a unique member of the glycoprotein hormone family as it elicits both follicle-stimulating hormone (FSH)-like and luteinizing hormone (LH)-like responses in non-equid species.
34482828	1	20	theme	BACKGROUND	219:228	arg1	gonadotropin					247:258	BACKGROUND Equine chorionic gonadotropin	219:258	BACKGROUND Equine chorionic gonadotropin (eCG)	219:264	BACKGROUND Equine chorionic gonadotropin (eCG), which comprises highly glycosylated α-subunit and β-subunit, is a unique member of the glycoprotein hormone family as it elicits both follicle-stimulating hormone (FSH)-like and luteinizing hormone (LH)-like responses in non-equid species.
34482828	9	21	theme	molecular	1656:1664	arg1	weight					1666:1671	the molecular weight	1652:1671	the molecular weight to approximately 5-10 kDa	1652:1697	Treatment with peptide-N-glycanase F markedly decreased the molecular weight to approximately 5-10 kDa.
34482828	0	22	link	O-linked	22:29	arg1	sites					45:49	N-linked and O-linked glycosylation sites	9:49	N-linked and O-linked glycosylation sites	9:49	Roles of N-linked and O-linked glycosylation sites in the activity of equine chorionic gonadotropin in cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating hormone receptor.
34482828	7	23	theme	expression	1364:1373	arg1	200-250 mIU/mL					1411:1424	200-250 mIU/mL	1411:1424	200-250 mIU/mL	1411:1424	The expression levels of all mutants were similar (200-250 mIU/mL) from days 3 to 7 post-transfection.
34482828	7	23	theme	expression	1364:1373	arg1	similar					1402:1408	similar	1402:1408	similar	1402:1408	The expression levels of all mutants were similar (200-250 mIU/mL) from days 3 to 7 post-transfection.
34482828	7	23	theme	expression	1364:1373	arg1	levels					1375:1380	The expression levels	1360:1380	The expression levels of all mutants	1360:1395	The expression levels of all mutants were similar (200-250 mIU/mL) from days 3 to 7 post-transfection.
34482828	4	24	theme	rat	1071:1073	arg1	receptor					1079:1086	rat FSH receptor	1071:1086	rat FSH receptor (rFSHR)	1071:1094	The FSH-like and LH-like activities of the mutants were examined using CHO-K1 cells expressing rat lutropin/CG receptor (rLH/CGR) and rat FSH receptor (rFSHR).
34482828	4	24	theme	rat	1071:1073	arg1	rFSHR					1089:1093	rFSHR	1089:1093	rFSHR	1089:1093	The FSH-like and LH-like activities of the mutants were examined using CHO-K1 cells expressing rat lutropin/CG receptor (rLH/CGR) and rat FSH receptor (rFSHR).
34482828	1	25	theme	follicle-stimulating	401:420	arg1	FSH					431:433	FSH	431:433	FSH	431:433	BACKGROUND Equine chorionic gonadotropin (eCG), which comprises highly glycosylated α-subunit and β-subunit, is a unique member of the glycoprotein hormone family as it elicits both follicle-stimulating hormone (FSH)-like and luteinizing hormone (LH)-like responses in non-equid species.
34482828	1	25	theme	follicle-stimulating	401:420	arg1	hormone					422:428	follicle-stimulating hormone	401:428	follicle-stimulating hormone (FSH)	401:434	BACKGROUND Equine chorionic gonadotropin (eCG), which comprises highly glycosylated α-subunit and β-subunit, is a unique member of the glycoprotein hormone family as it elicits both follicle-stimulating hormone (FSH)-like and luteinizing hormone (LH)-like responses in non-equid species.
34482828	2	26	with	substitution	642:653	arg1	Gln					682:684	Gln	682:684	Gln	682:684	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	1	27	theme	chorionic	237:245	arg1	member					340:345	a unique member	331:345	a unique member of the glycoprotein hormone family	331:380	BACKGROUND Equine chorionic gonadotropin (eCG), which comprises highly glycosylated α-subunit and β-subunit, is a unique member of the glycoprotein hormone family as it elicits both follicle-stimulating hormone (FSH)-like and luteinizing hormone (LH)-like responses in non-equid species.
34482828	1	27	theme	chorionic	237:245	arg1	eCG					261:263	eCG	261:263	eCG	261:263	BACKGROUND Equine chorionic gonadotropin (eCG), which comprises highly glycosylated α-subunit and β-subunit, is a unique member of the glycoprotein hormone family as it elicits both follicle-stimulating hormone (FSH)-like and luteinizing hormone (LH)-like responses in non-equid species.
34482828	1	27	theme	chorionic	237:245	arg1	gonadotropin					247:258	BACKGROUND Equine chorionic gonadotropin	219:258	BACKGROUND Equine chorionic gonadotropin (eCG)	219:264	BACKGROUND Equine chorionic gonadotropin (eCG), which comprises highly glycosylated α-subunit and β-subunit, is a unique member of the glycoprotein hormone family as it elicits both follicle-stimulating hormone (FSH)-like and luteinizing hormone (LH)-like responses in non-equid species.
34482828	2	28	theme	sites	558:562	arg1	function					533:540	the biological function	518:540	the biological function of glycosylated sites in eCG	518:569	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	17	29	theme	eCG	2555:2557	arg1	activities					2541:2550	both LH-like and FSH-like activities	2515:2550	both LH-like and FSH-like activities of eCG	2515:2557	These findings improved our understanding of the mechanisms underlying both LH-like and FSH-like activities of eCG.
34482828	2	30	theme	CTP	775:777	arg1	region					780:785	the carboxyl-terminal peptide (CTP) region	744:785	the carboxyl-terminal peptide (CTP) region of the β-subunit	744:802	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	0	31	theme	luteinizing	124:134	arg1	receptor					167:174	rat luteinizing hormone/chorionic gonadotropin receptor	120:174	rat luteinizing hormone/chorionic gonadotropin receptor	120:174	Roles of N-linked and O-linked glycosylation sites in the activity of equine chorionic gonadotropin in cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating hormone receptor.
34482828	2	32	theme	glycosylation	586:598	arg1	mutants					605:611	the following glycosylation site mutants	572:611	the following glycosylation site mutants	572:611	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	0	33	theme	follicle-stimulating	180:199	arg1	receptor					209:216	follicle-stimulating hormone receptor	180:216	follicle-stimulating hormone receptor	180:216	Roles of N-linked and O-linked glycosylation sites in the activity of equine chorionic gonadotropin in cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating hormone receptor.
34482828	11	34	theme	mutants	1813:1819	arg1	activity					1789:1796	The signal transduction activity	1765:1796	The signal transduction activity of both double mutants	1765:1819	The signal transduction activity of both double mutants was completely impaired.
34482828	0	35	theme	gonadotropin	154:165	arg1	receptor					167:174	rat luteinizing hormone/chorionic gonadotropin receptor	120:174	rat luteinizing hormone/chorionic gonadotropin receptor	120:174	Roles of N-linked and O-linked glycosylation sites in the activity of equine chorionic gonadotropin in cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating hormone receptor.
34482828	2	36	gly	glycosylation	586:598	arg2	site					600:603	the following glycosylation site mutants	572:611	the following glycosylation site mutants	572:611	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	2	36	gly	glycosylation	586:598	arg2	mutants					605:611	the following glycosylation site mutants	572:611	the following glycosylation site mutants	572:611	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	3	37	theme	eCG	849:851	arg1	mutants					863:869	The recombinant eCG (rec-eCG) mutants	833:869	The recombinant eCG (rec-eCG) mutants	833:869	The recombinant eCG (rec-eCG) mutants were expressed in Chinese hamster ovary suspension (CHO-S) cells.
34482828	2	38	from	function	533:540	arg1	eCG					567:569	eCG	567:569	eCG	567:569	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	2	39	theme	site	736:739	arg1	mutant					825:830	double mutant	818:830	double mutant	818:830	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	2	39	theme	site	736:739	arg1	deletion					697:704	deletion	697:704	deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit	697:802	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	5	40	theme	cell	1180:1183	arg1	medium					1193:1198	the CHO-S cell culture medium	1170:1198	the CHO-S cell culture medium	1170:1198	RESULTS Both rec-eCGβ/α and rec-eCGβ/αΔ56 were efficiently secreted into the CHO-S cell culture medium on day 1 post-transfection.
34482828	16	41	link	N-linked	2321:2328	arg1	site					2344:2347	the N-linked glycosylation site	2317:2347	the N-linked glycosylation site	2317:2347	The findings of this study suggest that the LH-like and FSH-like activities of eCG are regulated by the N-linked glycosylation site at Asn56 of the eCG α-subunit and/or by the O-linked glycosylation sites of the eCG β-subunit.
34482828	2	42	theme	O-linked	713:720	arg1	site					736:739	the O-linked glycosylation site	709:739	the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit	709:802	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	16	43	theme	glycosylation	2330:2342	arg1	site					2344:2347	the N-linked glycosylation site	2317:2347	the N-linked glycosylation site	2317:2347	The findings of this study suggest that the LH-like and FSH-like activities of eCG are regulated by the N-linked glycosylation site at Asn56 of the eCG α-subunit and/or by the O-linked glycosylation sites of the eCG β-subunit.
34482828	11	44	theme	signal	1769:1774	arg1	activity					1789:1796	The signal transduction activity	1765:1796	The signal transduction activity of both double mutants	1765:1819	The signal transduction activity of both double mutants was completely impaired.
34482828	1	45	theme	hormone	367:373	arg1	family					375:380	the glycoprotein hormone family	350:380	the glycoprotein hormone family	350:380	BACKGROUND Equine chorionic gonadotropin (eCG), which comprises highly glycosylated α-subunit and β-subunit, is a unique member of the glycoprotein hormone family as it elicits both follicle-stimulating hormone (FSH)-like and luteinizing hormone (LH)-like responses in non-equid species.
34482828	9	46	theme	peptide-N-glycanase	1611:1629	arg1	F					1631:1631	peptide-N-glycanase F	1611:1631	peptide-N-glycanase F	1611:1631	Treatment with peptide-N-glycanase F markedly decreased the molecular weight to approximately 5-10 kDa.
34482828	3	47	theme	rec-eCG	854:860	arg1	mutants					863:869	The recombinant eCG (rec-eCG) mutants	833:869	The recombinant eCG (rec-eCG) mutants	833:869	The recombinant eCG (rec-eCG) mutants were expressed in Chinese hamster ovary suspension (CHO-S) cells.
34482828	0	48	from	Roles	0:4	arg1	activity					58:65	the activity	54:65	the activity of equine chorionic gonadotropin in cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating hormone receptor	54:216	Roles of N-linked and O-linked glycosylation sites in the activity of equine chorionic gonadotropin in cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating hormone receptor.
34482828	0	49	theme	chorionic	77:85	arg1	gonadotropin					87:98	equine chorionic gonadotropin	70:98	equine chorionic gonadotropin	70:98	Roles of N-linked and O-linked glycosylation sites in the activity of equine chorionic gonadotropin in cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating hormone receptor.
34482828	12	50	theme	pivotal	1923:1929	arg1	role					1931:1934	a pivotal role	1921:1934	a pivotal role	1921:1934	This indicated that the glycosylation site at Asn56 of the α-subunit plays a pivotal role in the LH-like activity of eCG.
34482828	3	51	theme	Chinese	889:895	arg1	cells					930:934	Chinese hamster ovary suspension (CHO-S) cells	889:934	Chinese hamster ovary suspension (CHO-S) cells	889:934	The recombinant eCG (rec-eCG) mutants were expressed in Chinese hamster ovary suspension (CHO-S) cells.
34482828	12	52	from	Asn56	1892:1896	arg1	site					1884:1887	the glycosylation site	1866:1887	the glycosylation site at Asn56 of the α-subunit	1866:1913	This indicated that the glycosylation site at Asn56 of the α-subunit plays a pivotal role in the LH-like activity of eCG.
34482828	2	53	theme	biological	522:531	arg1	function					533:540	the biological function	518:540	the biological function of glycosylated sites in eCG	518:569	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	12	54	gly	glycosylation	1870:1882	arg2	Asn56					1892:1896	Asn56	1892:1896	Asn56 of the α-subunit	1892:1913	This indicated that the glycosylation site at Asn56 of the α-subunit plays a pivotal role in the LH-like activity of eCG.
34482828	12	54	gly	glycosylation	1870:1882	arg2	site					1884:1887	the glycosylation site	1866:1887	the glycosylation site at Asn56 of the α-subunit	1866:1913	This indicated that the glycosylation site at Asn56 of the α-subunit plays a pivotal role in the LH-like activity of eCG.
34482828	6	55	theme	glycosylation	1317:1329	arg1	sites					1331:1335	approximately 12 O-linked glycosylation sites	1291:1335	approximately 12 O-linked glycosylation sites	1291:1335	However, the secretion of eCGβ-D/α and eCGβ-D/αΔ56, which lack approximately 12 O-linked glycosylation sites, was slightly delayed.
34482828	7	56	dep	post-transfection	1444:1460	arg1	to					1439:1440	to	1439:1440	to	1439:1440	The expression levels of all mutants were similar (200-250 mIU/mL) from days 3 to 7 post-transfection.
34482828	14	57	theme	FSH-like	2098:2105	arg1	activities					2107:2116	downregulated LH-like and FSH-like activities	2072:2116	downregulated LH-like and FSH-like activities	2072:2116	eCGβ-D/α exhibited markedly downregulated LH-like and FSH-like activities.
34482828	2	58	theme	β-subunit	794:802	arg1	region					780:785	the carboxyl-terminal peptide (CTP) region	744:785	the carboxyl-terminal peptide (CTP) region of the β-subunit	744:802	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	2	59	dep	deletion	697:704	arg1	eCGβ-D/αΔ56					805:815	eCGβ-D/αΔ56	805:815	eCGβ-D/αΔ56	805:815	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	14	60	theme	LH-like	2086:2092	arg1	activities					2107:2116	downregulated LH-like and FSH-like activities	2072:2116	downregulated LH-like and FSH-like activities	2072:2116	eCGβ-D/α exhibited markedly downregulated LH-like and FSH-like activities.
34482828	0	61	theme	N-linked	9:16	arg1	sites					45:49	N-linked and O-linked glycosylation sites	9:49	N-linked and O-linked glycosylation sites	9:49	Roles of N-linked and O-linked glycosylation sites in the activity of equine chorionic gonadotropin in cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating hormone receptor.
34482828	3	62	theme	ovary	905:909	arg1	cells					930:934	Chinese hamster ovary suspension (CHO-S) cells	889:934	Chinese hamster ovary suspension (CHO-S) cells	889:934	The recombinant eCG (rec-eCG) mutants were expressed in Chinese hamster ovary suspension (CHO-S) cells.
34482828	8	63	theme	β-D/α	1525:1529	arg1	weight					1477:1482	The molecular weight	1463:1482	The molecular weight of rec-eCGβ/α, rec-eCGβ/αΔ56 and rec-eCG β-D/α	1463:1529	The molecular weight of rec-eCGβ/α, rec-eCGβ/αΔ56 and rec-eCG β-D/α were in the ranges of 40-45, 37-42, and 34-36 kDa, respectively.
34482828	12	64	theme	LH-like	1943:1949	arg1	activity					1951:1958	the LH-like activity	1939:1958	the LH-like activity of eCG	1939:1965	This indicated that the glycosylation site at Asn56 of the α-subunit plays a pivotal role in the LH-like activity of eCG.
34482828	16	65	theme	eCG	2296:2298	arg1	activities					2282:2291	the LH-like and FSH-like activities	2257:2291	the LH-like and FSH-like activities of eCG	2257:2298	The findings of this study suggest that the LH-like and FSH-like activities of eCG are regulated by the N-linked glycosylation site at Asn56 of the eCG α-subunit and/or by the O-linked glycosylation sites of the eCG β-subunit.
34482828	0	66	theme	O-linked	22:29	arg1	sites					45:49	N-linked and O-linked glycosylation sites	9:49	N-linked and O-linked glycosylation sites	9:49	Roles of N-linked and O-linked glycosylation sites in the activity of equine chorionic gonadotropin in cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating hormone receptor.
34482828	15	67	theme	potent	2151:2156	arg1	activity					2169:2176	potent biological activity	2151:2176	potent biological activity in cells expressing rLH/CGR and rFSHR	2151:2214	CONCLUSIONS Rec-eCGβ/α exhibits potent biological activity in cells expressing rLH/CGR and rFSHR.
34482828	16	68	theme	glycosylation	2402:2414	arg1	β-subunit					2433:2441	the eCG β-subunit	2425:2441	the eCG β-subunit	2425:2441	The findings of this study suggest that the LH-like and FSH-like activities of eCG are regulated by the N-linked glycosylation site at Asn56 of the eCG α-subunit and/or by the O-linked glycosylation sites of the eCG β-subunit.
34482828	16	68	theme	glycosylation	2402:2414	arg1	sites					2416:2420	the O-linked glycosylation sites	2389:2420	the O-linked glycosylation sites of the eCG β-subunit	2389:2441	The findings of this study suggest that the LH-like and FSH-like activities of eCG are regulated by the N-linked glycosylation site at Asn56 of the eCG α-subunit and/or by the O-linked glycosylation sites of the eCG β-subunit.
34482828	0	69	theme	sites	45:49	arg1	Roles					0:4	Roles	0:4	Roles of N-linked and O-linked glycosylation sites in the activity of equine chorionic gonadotropin in cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating hormone receptor.	0:217	Roles of N-linked and O-linked glycosylation sites in the activity of equine chorionic gonadotropin in cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating hormone receptor.
34482828	16	70	gly	glycosylation	2330:2342	arg2	site					2344:2347	the N-linked glycosylation site	2317:2347	the N-linked glycosylation site	2317:2347	The findings of this study suggest that the LH-like and FSH-like activities of eCG are regulated by the N-linked glycosylation site at Asn56 of the eCG α-subunit and/or by the O-linked glycosylation sites of the eCG β-subunit.
34482828	0	71	gly	glycosylation	31:43	arg2	sites					45:49	N-linked and O-linked glycosylation sites	9:49	N-linked and O-linked glycosylation sites	9:49	Roles of N-linked and O-linked glycosylation sites in the activity of equine chorionic gonadotropin in cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating hormone receptor.
34482828	6	72	link	O-linked	1308:1315	arg1	sites					1331:1335	approximately 12 O-linked glycosylation sites	1291:1335	approximately 12 O-linked glycosylation sites	1291:1335	However, the secretion of eCGβ-D/α and eCGβ-D/αΔ56, which lack approximately 12 O-linked glycosylation sites, was slightly delayed.
34482828	8	73	theme	40-45	1553:1557	arg1	ranges					1543:1548	the ranges	1539:1548	the ranges of 40-45, 37-42, and 34-36 kDa, respectively	1539:1593	The molecular weight of rec-eCGβ/α, rec-eCGβ/αΔ56 and rec-eCG β-D/α were in the ranges of 40-45, 37-42, and 34-36 kDa, respectively.
34482828	16	74	theme	eCG	2429:2431	arg1	β-subunit					2433:2441	the eCG β-subunit	2425:2441	the eCG β-subunit	2425:2441	The findings of this study suggest that the LH-like and FSH-like activities of eCG are regulated by the N-linked glycosylation site at Asn56 of the eCG α-subunit and/or by the O-linked glycosylation sites of the eCG β-subunit.
34482828	12	75	theme	eCG	1963:1965	arg1	activity					1951:1958	the LH-like activity	1939:1958	the LH-like activity of eCG	1939:1965	This indicated that the glycosylation site at Asn56 of the α-subunit plays a pivotal role in the LH-like activity of eCG.
34482828	4	76	theme	rat	1032:1034	arg1	rLH/CGR					1058:1064	rLH/CGR	1058:1064	rLH/CGR	1058:1064	The FSH-like and LH-like activities of the mutants were examined using CHO-K1 cells expressing rat lutropin/CG receptor (rLH/CGR) and rat FSH receptor (rFSHR).
34482828	4	76	theme	rat	1032:1034	arg1	receptor					1048:1055	rat lutropin/CG receptor	1032:1055	rat lutropin/CG receptor (rLH/CGR)	1032:1065	The FSH-like and LH-like activities of the mutants were examined using CHO-K1 cells expressing rat lutropin/CG receptor (rLH/CGR) and rat FSH receptor (rFSHR).
34482828	8	77	theme	37-42	1560:1564	arg1	ranges					1543:1548	the ranges	1539:1548	the ranges of 40-45, 37-42, and 34-36 kDa, respectively	1539:1593	The molecular weight of rec-eCGβ/α, rec-eCGβ/αΔ56 and rec-eCG β-D/α were in the ranges of 40-45, 37-42, and 34-36 kDa, respectively.
34482828	6	78	theme	eCGβ-D/α	1254:1261	arg1	secretion					1241:1249	the secretion	1237:1249	the secretion	1237:1249	However, the secretion of eCGβ-D/α and eCGβ-D/αΔ56, which lack approximately 12 O-linked glycosylation sites, was slightly delayed.
34482828	2	79	theme	double	818:823	arg1	mutant					825:830	double mutant	818:830	double mutant	818:830	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	2	79	theme	double	818:823	arg1	deletion					697:704	deletion	697:704	deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit	697:802	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	16	80	theme	study	2238:2242	arg1	findings					2221:2228	The findings	2217:2228	The findings of this study	2217:2242	The findings of this study suggest that the LH-like and FSH-like activities of eCG are regulated by the N-linked glycosylation site at Asn56 of the eCG α-subunit and/or by the O-linked glycosylation sites of the eCG β-subunit.
34482828	15	81	theme	CONCLUSIONS	2119:2129	arg1	Rec-eCGβ/α					2131:2140	CONCLUSIONS Rec-eCGβ/α	2119:2140	CONCLUSIONS Rec-eCGβ/α	2119:2140	CONCLUSIONS Rec-eCGβ/α exhibits potent biological activity in cells expressing rLH/CGR and rFSHR.
34482828	1	82	theme	glycosylated	290:301	arg1	α-subunit					303:311	highly glycosylated α-subunit	283:311	highly glycosylated α-subunit	283:311	BACKGROUND Equine chorionic gonadotropin (eCG), which comprises highly glycosylated α-subunit and β-subunit, is a unique member of the glycoprotein hormone family as it elicits both follicle-stimulating hormone (FSH)-like and luteinizing hormone (LH)-like responses in non-equid species.
34482828	2	83	theme	site	600:603	arg1	mutants					605:611	the following glycosylation site mutants	572:611	the following glycosylation site mutants	572:611	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	16	84	theme	eCG	2365:2367	arg1	α-subunit					2369:2377	the eCG α-subunit	2361:2377	the eCG α-subunit	2361:2377	The findings of this study suggest that the LH-like and FSH-like activities of eCG are regulated by the N-linked glycosylation site at Asn56 of the eCG α-subunit and/or by the O-linked glycosylation sites of the eCG β-subunit.
34482828	10	85	theme	downregulated	1733:1745	arg1	activity					1755:1762	downregulated LH-like activity	1733:1762	downregulated LH-like activity	1733:1762	Rec-eCGβ/αΔ56 exhibited markedly downregulated LH-like activity.
34482828	2	86	theme	carboxyl-terminal	748:764	arg1	region					780:785	the carboxyl-terminal peptide (CTP) region	744:785	the carboxyl-terminal peptide (CTP) region of the β-subunit	744:802	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	16	87	gly	glycosylation	2402:2414	arg1	β-subunit					2433:2441	the eCG β-subunit	2425:2441	the eCG β-subunit	2425:2441	The findings of this study suggest that the LH-like and FSH-like activities of eCG are regulated by the N-linked glycosylation site at Asn56 of the eCG α-subunit and/or by the O-linked glycosylation sites of the eCG β-subunit.
34482828	16	87	gly	glycosylation	2402:2414	arg2	β-subunit					2433:2441	the eCG β-subunit	2425:2441	the eCG β-subunit	2425:2441	The findings of this study suggest that the LH-like and FSH-like activities of eCG are regulated by the N-linked glycosylation site at Asn56 of the eCG α-subunit and/or by the O-linked glycosylation sites of the eCG β-subunit.
34482828	16	87	gly	glycosylation	2402:2414	arg2	sites					2416:2420	the O-linked glycosylation sites	2389:2420	the O-linked glycosylation sites of the eCG β-subunit	2389:2441	The findings of this study suggest that the LH-like and FSH-like activities of eCG are regulated by the N-linked glycosylation site at Asn56 of the eCG α-subunit and/or by the O-linked glycosylation sites of the eCG β-subunit.
34482828	4	88	theme	FSH	1075:1077	arg1	receptor					1079:1086	rat FSH receptor	1071:1086	rat FSH receptor (rFSHR)	1071:1094	The FSH-like and LH-like activities of the mutants were examined using CHO-K1 cells expressing rat lutropin/CG receptor (rLH/CGR) and rat FSH receptor (rFSHR).
34482828	4	88	theme	FSH	1075:1077	arg1	rFSHR					1089:1093	rFSHR	1089:1093	rFSHR	1089:1093	The FSH-like and LH-like activities of the mutants were examined using CHO-K1 cells expressing rat lutropin/CG receptor (rLH/CGR) and rat FSH receptor (rFSHR).
34482828	11	89	theme	double	1806:1811	arg1	mutants					1813:1819	both double mutants	1801:1819	both double mutants	1801:1819	The signal transduction activity of both double mutants was completely impaired.
34482828	6	90	theme	O-linked	1308:1315	arg1	sites					1331:1335	approximately 12 O-linked glycosylation sites	1291:1335	approximately 12 O-linked glycosylation sites	1291:1335	However, the secretion of eCGβ-D/α and eCGβ-D/αΔ56, which lack approximately 12 O-linked glycosylation sites, was slightly delayed.
34482828	1	91	theme	non-equid	488:496	arg1	species					498:504	non-equid species	488:504	non-equid species	488:504	BACKGROUND Equine chorionic gonadotropin (eCG), which comprises highly glycosylated α-subunit and β-subunit, is a unique member of the glycoprotein hormone family as it elicits both follicle-stimulating hormone (FSH)-like and luteinizing hormone (LH)-like responses in non-equid species.
34482828	1	92	theme	Equine	230:235	arg1	member					340:345	a unique member	331:345	a unique member of the glycoprotein hormone family	331:380	BACKGROUND Equine chorionic gonadotropin (eCG), which comprises highly glycosylated α-subunit and β-subunit, is a unique member of the glycoprotein hormone family as it elicits both follicle-stimulating hormone (FSH)-like and luteinizing hormone (LH)-like responses in non-equid species.
34482828	1	92	theme	Equine	230:235	arg1	eCG					261:263	eCG	261:263	eCG	261:263	BACKGROUND Equine chorionic gonadotropin (eCG), which comprises highly glycosylated α-subunit and β-subunit, is a unique member of the glycoprotein hormone family as it elicits both follicle-stimulating hormone (FSH)-like and luteinizing hormone (LH)-like responses in non-equid species.
34482828	1	92	theme	Equine	230:235	arg1	gonadotropin					247:258	BACKGROUND Equine chorionic gonadotropin	219:258	BACKGROUND Equine chorionic gonadotropin (eCG)	219:264	BACKGROUND Equine chorionic gonadotropin (eCG), which comprises highly glycosylated α-subunit and β-subunit, is a unique member of the glycoprotein hormone family as it elicits both follicle-stimulating hormone (FSH)-like and luteinizing hormone (LH)-like responses in non-equid species.
34482828	17	93	theme	mechanisms	2493:2502	arg1	understanding					2472:2484	our understanding	2468:2484	our understanding of the mechanisms underlying both LH-like and FSH-like activities of eCG	2468:2557	These findings improved our understanding of the mechanisms underlying both LH-like and FSH-like activities of eCG.
34482828	16	94	link	O-linked	2393:2400	arg1	β-subunit					2433:2441	the eCG β-subunit	2425:2441	the eCG β-subunit	2425:2441	The findings of this study suggest that the LH-like and FSH-like activities of eCG are regulated by the N-linked glycosylation site at Asn56 of the eCG α-subunit and/or by the O-linked glycosylation sites of the eCG β-subunit.
34482828	16	94	link	O-linked	2393:2400	arg1	sites					2416:2420	the O-linked glycosylation sites	2389:2420	the O-linked glycosylation sites of the eCG β-subunit	2389:2441	The findings of this study suggest that the LH-like and FSH-like activities of eCG are regulated by the N-linked glycosylation site at Asn56 of the eCG α-subunit and/or by the O-linked glycosylation sites of the eCG β-subunit.
34482828	0	95	theme	hormone/chorionic	136:152	arg1	receptor					167:174	rat luteinizing hormone/chorionic gonadotropin receptor	120:174	rat luteinizing hormone/chorionic gonadotropin receptor	120:174	Roles of N-linked and O-linked glycosylation sites in the activity of equine chorionic gonadotropin in cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating hormone receptor.
34482828	15	96	theme	biological	2158:2167	arg1	activity					2169:2176	potent biological activity	2151:2176	potent biological activity in cells expressing rLH/CGR and rFSHR	2151:2214	CONCLUSIONS Rec-eCGβ/α exhibits potent biological activity in cells expressing rLH/CGR and rFSHR.
34482828	2	97	theme	glycosylated	545:556	arg1	sites					558:562	glycosylated sites	545:562	glycosylated sites	545:562	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	0	98	from	activity	58:65	arg1	cells					103:107	cells	103:107	cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating hormone receptor	103:216	Roles of N-linked and O-linked glycosylation sites in the activity of equine chorionic gonadotropin in cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating hormone receptor.
34482828	0	99	theme	rat	120:122	arg1	receptor					167:174	rat luteinizing hormone/chorionic gonadotropin receptor	120:174	rat luteinizing hormone/chorionic gonadotropin receptor	120:174	Roles of N-linked and O-linked glycosylation sites in the activity of equine chorionic gonadotropin in cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating hormone receptor.
34482828	2	100	theme	following	576:584	arg1	mutants					605:611	the following glycosylation site mutants	572:611	the following glycosylation site mutants	572:611	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	6	101	theme	eCGβ-D/αΔ56	1267:1277	arg1	secretion					1241:1249	the secretion	1237:1249	the secretion	1237:1249	However, the secretion of eCGβ-D/α and eCGβ-D/αΔ56, which lack approximately 12 O-linked glycosylation sites, was slightly delayed.
34482828	1	102	theme	-like	435:439	arg1	responses					475:483	both follicle-stimulating hormone (FSH)-like and luteinizing hormone (LH)-like responses	396:483	both follicle-stimulating hormone (FSH)-like and luteinizing hormone (LH)-like responses	396:483	BACKGROUND Equine chorionic gonadotropin (eCG), which comprises highly glycosylated α-subunit and β-subunit, is a unique member of the glycoprotein hormone family as it elicits both follicle-stimulating hormone (FSH)-like and luteinizing hormone (LH)-like responses in non-equid species.
34482828	7	103	dep	similar	1402:1408	arg1	200-250 mIU/mL					1411:1424	200-250 mIU/mL	1411:1424	200-250 mIU/mL	1411:1424	The expression levels of all mutants were similar (200-250 mIU/mL) from days 3 to 7 post-transfection.
34482828	7	103	dep	similar	1402:1408	arg1	similar					1402:1408	similar	1402:1408	similar	1402:1408	The expression levels of all mutants were similar (200-250 mIU/mL) from days 3 to 7 post-transfection.
34482828	7	103	dep	similar	1402:1408	arg1	levels					1375:1380	The expression levels	1360:1380	The expression levels of all mutants	1360:1395	The expression levels of all mutants were similar (200-250 mIU/mL) from days 3 to 7 post-transfection.
34482828	16	104	theme	N-linked	2321:2328	arg1	site					2344:2347	the N-linked glycosylation site	2317:2347	the N-linked glycosylation site	2317:2347	The findings of this study suggest that the LH-like and FSH-like activities of eCG are regulated by the N-linked glycosylation site at Asn56 of the eCG α-subunit and/or by the O-linked glycosylation sites of the eCG β-subunit.
34482828	1	105	theme	luteinizing	445:455	arg1	LH					466:467	LH	466:467	LH	466:467	BACKGROUND Equine chorionic gonadotropin (eCG), which comprises highly glycosylated α-subunit and β-subunit, is a unique member of the glycoprotein hormone family as it elicits both follicle-stimulating hormone (FSH)-like and luteinizing hormone (LH)-like responses in non-equid species.
34482828	1	105	theme	luteinizing	445:455	arg1	hormone					457:463	luteinizing hormone	445:463	luteinizing hormone (LH)	445:468	BACKGROUND Equine chorionic gonadotropin (eCG), which comprises highly glycosylated α-subunit and β-subunit, is a unique member of the glycoprotein hormone family as it elicits both follicle-stimulating hormone (FSH)-like and luteinizing hormone (LH)-like responses in non-equid species.
34482828	3	106	theme	recombinant	837:847	arg1	mutants					863:869	The recombinant eCG (rec-eCG) mutants	833:869	The recombinant eCG (rec-eCG) mutants	833:869	The recombinant eCG (rec-eCG) mutants were expressed in Chinese hamster ovary suspension (CHO-S) cells.
34482828	4	107	theme	FSH-like	941:948	arg1	activities					962:971	The FSH-like and LH-like activities	937:971	The FSH-like and LH-like activities of the mutants	937:986	The FSH-like and LH-like activities of the mutants were examined using CHO-K1 cells expressing rat lutropin/CG receptor (rLH/CGR) and rat FSH receptor (rFSHR).
34482828	2	108	theme	glycosylation	722:734	arg1	site					736:739	the O-linked glycosylation site	709:739	the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit	709:802	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	0	109	theme	equine	70:75	arg1	gonadotropin					87:98	equine chorionic gonadotropin	70:98	equine chorionic gonadotropin	70:98	Roles of N-linked and O-linked glycosylation sites in the activity of equine chorionic gonadotropin in cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating hormone receptor.
34482828	1	110	theme	glycoprotein	354:365	arg1	family					375:380	the glycoprotein hormone family	350:380	the glycoprotein hormone family	350:380	BACKGROUND Equine chorionic gonadotropin (eCG), which comprises highly glycosylated α-subunit and β-subunit, is a unique member of the glycoprotein hormone family as it elicits both follicle-stimulating hormone (FSH)-like and luteinizing hormone (LH)-like responses in non-equid species.
34482828	2	111	gly	glycosylated	545:556	arg1	sites					558:562	glycosylated sites	545:562	glycosylated sites	545:562	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	4	112	theme	LH-like	954:960	arg1	activities					962:971	The FSH-like and LH-like activities	937:971	The FSH-like and LH-like activities of the mutants	937:986	The FSH-like and LH-like activities of the mutants were examined using CHO-K1 cells expressing rat lutropin/CG receptor (rLH/CGR) and rat FSH receptor (rFSHR).
34482828	2	113	theme	α-subunit	667:675	arg1	Asn56					658:662	Asn56	658:662	Asn56 of α-subunit	658:675	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	11	114	theme	transduction	1776:1787	arg1	activity					1789:1796	The signal transduction activity	1765:1796	The signal transduction activity of both double mutants	1765:1819	The signal transduction activity of both double mutants was completely impaired.
34482828	0	115	theme	gonadotropin	87:98	arg1	activity					58:65	the activity	54:65	the activity of equine chorionic gonadotropin in cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating hormone receptor	54:216	Roles of N-linked and O-linked glycosylation sites in the activity of equine chorionic gonadotropin in cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating hormone receptor.
34482828	1	116	theme	family	375:380	arg1	gonadotropin					247:258	BACKGROUND Equine chorionic gonadotropin	219:258	BACKGROUND Equine chorionic gonadotropin (eCG)	219:264	BACKGROUND Equine chorionic gonadotropin (eCG), which comprises highly glycosylated α-subunit and β-subunit, is a unique member of the glycoprotein hormone family as it elicits both follicle-stimulating hormone (FSH)-like and luteinizing hormone (LH)-like responses in non-equid species.
34482828	1	116	theme	family	375:380	arg1	member					340:345	a unique member	331:345	a unique member of the glycoprotein hormone family	331:380	BACKGROUND Equine chorionic gonadotropin (eCG), which comprises highly glycosylated α-subunit and β-subunit, is a unique member of the glycoprotein hormone family as it elicits both follicle-stimulating hormone (FSH)-like and luteinizing hormone (LH)-like responses in non-equid species.
34482828	2	117	link	O-linked	713:720	arg1	site					736:739	the O-linked glycosylation site	709:739	the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit	709:802	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	8	118	theme	rec-eCGβ/α	1487:1496	arg1	weight					1477:1482	The molecular weight	1463:1482	The molecular weight of rec-eCGβ/α, rec-eCGβ/αΔ56 and rec-eCG β-D/α	1463:1529	The molecular weight of rec-eCGβ/α, rec-eCGβ/αΔ56 and rec-eCG β-D/α were in the ranges of 40-45, 37-42, and 34-36 kDa, respectively.
34482828	2	119	theme	Asn56	658:662	arg1	substitution					642:653	substitution	642:653	substitution of Asn56 of α-subunit with Gln	642:684	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	16	120	theme	LH-like	2261:2267	arg1	activities					2282:2291	the LH-like and FSH-like activities	2257:2291	the LH-like and FSH-like activities of eCG	2257:2298	The findings of this study suggest that the LH-like and FSH-like activities of eCG are regulated by the N-linked glycosylation site at Asn56 of the eCG α-subunit and/or by the O-linked glycosylation sites of the eCG β-subunit.
34482828	3	121	theme	hamster	897:903	arg1	cells					930:934	Chinese hamster ovary suspension (CHO-S) cells	889:934	Chinese hamster ovary suspension (CHO-S) cells	889:934	The recombinant eCG (rec-eCG) mutants were expressed in Chinese hamster ovary suspension (CHO-S) cells.
34482828	8	122	theme	rec-eCGβ/αΔ56	1499:1511	arg1	weight					1477:1482	The molecular weight	1463:1482	The molecular weight of rec-eCGβ/α, rec-eCGβ/αΔ56 and rec-eCG β-D/α	1463:1529	The molecular weight of rec-eCGβ/α, rec-eCGβ/αΔ56 and rec-eCG β-D/α were in the ranges of 40-45, 37-42, and 34-36 kDa, respectively.
34482828	16	123	theme	FSH-like	2273:2280	arg1	activities					2282:2291	the LH-like and FSH-like activities	2257:2291	the LH-like and FSH-like activities of eCG	2257:2298	The findings of this study suggest that the LH-like and FSH-like activities of eCG are regulated by the N-linked glycosylation site at Asn56 of the eCG α-subunit and/or by the O-linked glycosylation sites of the eCG β-subunit.
34482828	12	124	theme	α-subunit	1905:1913	arg1	Asn56					1892:1896	Asn56	1892:1896	Asn56 of the α-subunit	1892:1913	This indicated that the glycosylation site at Asn56 of the α-subunit plays a pivotal role in the LH-like activity of eCG.
34482828	8	125	theme	rec-eCG	1517:1523	arg1	β-D/α					1525:1529	rec-eCG β-D/α	1517:1529	rec-eCG β-D/α	1517:1529	The molecular weight of rec-eCGβ/α, rec-eCGβ/αΔ56 and rec-eCG β-D/α were in the ranges of 40-45, 37-42, and 34-36 kDa, respectively.
34482828	12	126	theme	glycosylation	1870:1882	arg1	site					1884:1887	the glycosylation site	1866:1887	the glycosylation site at Asn56 of the α-subunit	1866:1913	This indicated that the glycosylation site at Asn56 of the α-subunit plays a pivotal role in the LH-like activity of eCG.
34482828	1	127	gly	glycosylated	290:301	arg1	α-subunit					303:311	highly glycosylated α-subunit	283:311	highly glycosylated α-subunit	283:311	BACKGROUND Equine chorionic gonadotropin (eCG), which comprises highly glycosylated α-subunit and β-subunit, is a unique member of the glycoprotein hormone family as it elicits both follicle-stimulating hormone (FSH)-like and luteinizing hormone (LH)-like responses in non-equid species.
34482828	3	128	theme	CHO-S	923:927	arg1	cells					930:934	Chinese hamster ovary suspension (CHO-S) cells	889:934	Chinese hamster ovary suspension (CHO-S) cells	889:934	The recombinant eCG (rec-eCG) mutants were expressed in Chinese hamster ovary suspension (CHO-S) cells.
34482828	4	129	theme	CHO-K1	1008:1013	arg1	cells					1015:1019	CHO-K1 cells	1008:1019	CHO-K1 cells expressing rat lutropin/CG receptor (rLH/CGR) and rat FSH receptor (rFSHR)	1008:1094	The FSH-like and LH-like activities of the mutants were examined using CHO-K1 cells expressing rat lutropin/CG receptor (rLH/CGR) and rat FSH receptor (rFSHR).
34482828	14	130	theme	downregulated	2072:2084	arg1	activities					2107:2116	downregulated LH-like and FSH-like activities	2072:2116	downregulated LH-like and FSH-like activities	2072:2116	eCGβ-D/α exhibited markedly downregulated LH-like and FSH-like activities.
34482828	0	131	theme	glycosylation	31:43	arg1	sites					45:49	N-linked and O-linked glycosylation sites	9:49	N-linked and O-linked glycosylation sites	9:49	Roles of N-linked and O-linked glycosylation sites in the activity of equine chorionic gonadotropin in cells expressing rat luteinizing hormone/chorionic gonadotropin receptor and follicle-stimulating hormone receptor.
34482828	2	132	gly	glycosylation	722:734	arg2	site					736:739	the O-linked glycosylation site	709:739	the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit	709:802	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	2	132	gly	glycosylation	722:734	arg2	region					780:785	the carboxyl-terminal peptide (CTP) region	744:785	the carboxyl-terminal peptide (CTP) region of the β-subunit	744:802	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	3	133	theme	suspension	911:920	arg1	cells					930:934	Chinese hamster ovary suspension (CHO-S) cells	889:934	Chinese hamster ovary suspension (CHO-S) cells	889:934	The recombinant eCG (rec-eCG) mutants were expressed in Chinese hamster ovary suspension (CHO-S) cells.
34482828	1	134	theme	unique	333:338	arg1	gonadotropin					247:258	BACKGROUND Equine chorionic gonadotropin	219:258	BACKGROUND Equine chorionic gonadotropin (eCG)	219:264	BACKGROUND Equine chorionic gonadotropin (eCG), which comprises highly glycosylated α-subunit and β-subunit, is a unique member of the glycoprotein hormone family as it elicits both follicle-stimulating hormone (FSH)-like and luteinizing hormone (LH)-like responses in non-equid species.
34482828	1	134	theme	unique	333:338	arg1	member					340:345	a unique member	331:345	a unique member of the glycoprotein hormone family	331:380	BACKGROUND Equine chorionic gonadotropin (eCG), which comprises highly glycosylated α-subunit and β-subunit, is a unique member of the glycoprotein hormone family as it elicits both follicle-stimulating hormone (FSH)-like and luteinizing hormone (LH)-like responses in non-equid species.
34482828	6	135	gly	glycosylation	1317:1329	arg2	12					1305:1306	12	1305:1306	12	1305:1306	However, the secretion of eCGβ-D/α and eCGβ-D/αΔ56, which lack approximately 12 O-linked glycosylation sites, was slightly delayed.
34482828	6	135	gly	glycosylation	1317:1329	arg2	sites					1331:1335	approximately 12 O-linked glycosylation sites	1291:1335	approximately 12 O-linked glycosylation sites	1291:1335	However, the secretion of eCGβ-D/α and eCGβ-D/αΔ56, which lack approximately 12 O-linked glycosylation sites, was slightly delayed.
34482828	4	136	theme	mutants	980:986	arg1	activities					962:971	The FSH-like and LH-like activities	937:971	The FSH-like and LH-like activities of the mutants	937:986	The FSH-like and LH-like activities of the mutants were examined using CHO-K1 cells expressing rat lutropin/CG receptor (rLH/CGR) and rat FSH receptor (rFSHR).
34482828	13	137	theme	mutants	2008:2014	arg1	activity					1992:1999	the FSH-like activity	1979:1999	the FSH-like activity of the mutants	1979:2014	Similarly, the FSH-like activity of the mutants was markedly downregulated.
34482828	2	138	dep	examine	510:516	arg1	eCGβ/αΔ56					631:639	eCGβ/αΔ56	631:639	eCGβ/αΔ56	631:639	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	17	139	theme	LH-like	2520:2526	arg1	activities					2541:2550	both LH-like and FSH-like activities	2515:2550	both LH-like and FSH-like activities of eCG	2515:2557	These findings improved our understanding of the mechanisms underlying both LH-like and FSH-like activities of eCG.
34482828	16	140	theme	β-subunit	2433:2441	arg1	β-subunit					2433:2441	the eCG β-subunit	2425:2441	the eCG β-subunit	2425:2441	The findings of this study suggest that the LH-like and FSH-like activities of eCG are regulated by the N-linked glycosylation site at Asn56 of the eCG α-subunit and/or by the O-linked glycosylation sites of the eCG β-subunit.
34482828	16	140	theme	β-subunit	2433:2441	arg1	sites					2416:2420	the O-linked glycosylation sites	2389:2420	the O-linked glycosylation sites of the eCG β-subunit	2389:2441	The findings of this study suggest that the LH-like and FSH-like activities of eCG are regulated by the N-linked glycosylation site at Asn56 of the eCG α-subunit and/or by the O-linked glycosylation sites of the eCG β-subunit.
34482828	17	141	theme	FSH-like	2532:2539	arg1	activities					2541:2550	both LH-like and FSH-like activities	2515:2550	both LH-like and FSH-like activities of eCG	2515:2557	These findings improved our understanding of the mechanisms underlying both LH-like and FSH-like activities of eCG.
34482828	2	142	from	region	780:785	arg1	mutant					825:830	double mutant	818:830	double mutant	818:830	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	2	142	from	region	780:785	arg1	site					736:739	the O-linked glycosylation site	709:739	the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit	709:802	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	2	142	from	region	780:785	arg1	deletion					697:704	deletion	697:704	deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit	697:802	To examine the biological function of glycosylated sites in eCG, the following glycosylation site mutants were constructed: eCGβ/αΔ56, substitution of Asn56 of α-subunit with Gln; eCGβ-D/α, deletion of the O-linked glycosylation site at the carboxyl-terminal peptide (CTP) region of the β-subunit; eCGβ-D/αΔ56, double mutant.
34482828	13	143	theme	FSH-like	1983:1990	arg1	activity					1992:1999	the FSH-like activity	1979:1999	the FSH-like activity of the mutants	1979:2014	Similarly, the FSH-like activity of the mutants was markedly downregulated.
32663007	17	0	theme	valuable	2926:2933	arg1	information					2935:2945	valuable information	2926:2945	valuable information for understanding the impact of environmentally relevant-level CeO2 NP exposure on soil microbial communities and metabolites with or without ferrous, which is needed to understand the ecological risk posed by long-term CeO2 NP exposure in rice-planted soil with rich ferrous	2926:3221	These results provide valuable information for understanding the impact of environmentally relevant-level CeO2 NP exposure on soil microbial communities and metabolites with or without ferrous, which is needed to understand the ecological risk posed by long-term CeO2 NP exposure in rice-planted soil with rich ferrous.
32663007	8	1	theme	phylum	1416:1421	arg1	level					1423:1427	the phylum level	1412:1427	the phylum level	1412:1427	NP decreased Firmicutes and increased Chloroflexi, Rokubacteria, and Thaumarchaeota abundances at the phylum level, which contributed to reduce soil cellulose activity.
32663007	11	2	theme	urea	2016:2019	arg1	cycle					2021:2025	urea cycle	2016:2025	urea cycle	2016:2025	After adding CeO2 NP with ferrous or not, the main metabolic changes were concentrated on fluctuations in starch and sucrose metabolism, nitrogen metabolism, sulfur metabolism, propanoate metabolism, fatty acid metabolism, and urea cycle.
32663007	9	3	theme	soil	1539:1542	arg1	pH					1544:1545	soil pH	1539:1545	soil pH	1539:1545	Additionally, CeO2 NP positively or negatively affected soil pH, Ce accumulation in root, and rice physiological properties (root-POD, stem-POD).
32663007	16	4	contain	containing	2847:2856	arg1	species					2839:2845	the most genus species	2824:2845	the most genus species containing 13 genera affecting soil metabolite profiles	2824:2901	Phylum Proteobacteria had the most genus species containing 13 genera affecting soil metabolite profiles.
32663007	16	4	contain	containing	2847:2856	arg2	genera					2861:2866	13 genera	2858:2866	13 genera affecting soil metabolite profiles	2858:2901	Phylum Proteobacteria had the most genus species containing 13 genera affecting soil metabolite profiles.
32663007	16	5	theme	metabolite	2883:2892	arg1	profiles					2894:2901	soil metabolite profiles	2878:2901	soil metabolite profiles	2878:2901	Phylum Proteobacteria had the most genus species containing 13 genera affecting soil metabolite profiles.
32663007	7	6	theme	rhizosphere	1214:1224	arg1	activities					1238:1247	rhizosphere soil enzyme activities	1214:1247	rhizosphere soil enzyme activities	1214:1247	Among rhizosphere soil enzyme activities, cellulose activity was the most sensitive for CeO2 NP exposure.
32663007	5	7	theme	significant	847:857	arg1	phyla					869:873	Several functionally significant bacterial phyla	826:873	Several functionally significant bacterial phyla	826:873	Several functionally significant bacterial phyla containing Proteobacteria and Bacteroidetes abundances, which were associated with carbon and nitrogen cycling, were promoted after CeO2 NP exposure with ferrous amendment.
32663007	17	8	theme	rice-planted	3187:3198	arg1	soil					3200:3203	rice-planted soil	3187:3203	rice-planted soil with rich ferrous	3187:3221	These results provide valuable information for understanding the impact of environmentally relevant-level CeO2 NP exposure on soil microbial communities and metabolites with or without ferrous, which is needed to understand the ecological risk posed by long-term CeO2 NP exposure in rice-planted soil with rich ferrous.
32663007	17	9	theme	exposure	3018:3025	arg1	impact					2969:2974	the impact	2965:2974	the impact of environmentally relevant-level CeO2 NP exposure on soil microbial communities and metabolites with or without ferrous, which is needed to understand the ecological risk posed by long-term CeO2 NP exposure in rice-planted soil with rich ferrous	2965:3221	These results provide valuable information for understanding the impact of environmentally relevant-level CeO2 NP exposure on soil microbial communities and metabolites with or without ferrous, which is needed to understand the ecological risk posed by long-term CeO2 NP exposure in rice-planted soil with rich ferrous.
32663007	15	10	theme	NP	2582:2583	arg1	presence					2565:2572	The presence	2561:2572	The presence of CeO2 NP	2561:2583	The presence of CeO2 NP mainly promoted fatty acids (hexanoic acid, nonanoic acid) and amino acid (oxoproline) and amine (diethanolamine) concentrations, which could be from the increased Proteobacteria abundance after CeO2 NP exposure.
32663007	12	11	contain	containing	2058:2067	arg1	alkane					2132:2137	alkane	2132:2137	alkane	2132:2137	The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure.
32663007	12	11	contain	containing	2058:2067	arg1	metabolites					2046:2056	The eight changed metabolites	2028:2056	The eight changed metabolites containing glycerol monstearate	2028:2088	The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure.
32663007	12	11	contain	containing	2058:2067	arg1	acid					2176:2179	stearic acid	2168:2179	stearic acid	2168:2179	The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure.
32663007	12	11	contain	containing	2058:2067	arg1	monopalmitin					2103:2114	monopalmitin	2103:2114	monopalmitin	2103:2114	The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure.
32663007	12	11	contain	containing	2058:2067	arg1	ethanol					2140:2146	ethanol	2140:2146	ethanol	2140:2146	The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure.
32663007	12	11	contain	containing	2058:2067	arg1	dicarboximide					2149:2161	dicarboximide	2149:2161	dicarboximide	2149:2161	The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure.
32663007	12	11	contain	containing	2058:2067	arg1	acid					2097:2100	boric acid	2091:2100	boric acid	2091:2100	The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure.
32663007	12	11	contain	containing	2058:2067	arg1	acid					2126:2129	palmitic acid	2117:2129	palmitic acid	2117:2129	The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure.
32663007	12	11	contain	containing	2058:2067	arg2	monstearate					2078:2088	glycerol monstearate	2069:2088	glycerol monstearate	2069:2088	The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure.
32663007	9	12	from	pH	1544:1545	arg1	root					1567:1570	root	1567:1570	root	1567:1570	Additionally, CeO2 NP positively or negatively affected soil pH, Ce accumulation in root, and rice physiological properties (root-POD, stem-POD).
32663007	11	13	theme	CeO2	1802:1805	arg1	NP					1807:1808	CeO2 NP	1802:1808	CeO2 NP	1802:1808	After adding CeO2 NP with ferrous or not, the main metabolic changes were concentrated on fluctuations in starch and sucrose metabolism, nitrogen metabolism, sulfur metabolism, propanoate metabolism, fatty acid metabolism, and urea cycle.
32663007	0	14	theme	Rice-Planted	134:145	arg1	Soils					147:151	Rice-Planted Soils	134:151	Rice-Planted Soils	134:151	Environmentally Relevant-Level CeO2 NP with Ferrous Amendment Alters Soil Bacterial Community Compositions and Metabolite Profiles in Rice-Planted Soils.
32663007	17	15	theme	soil	3030:3033	arg1	communities					3045:3055	soil microbial communities	3030:3055	soil microbial communities	3030:3055	These results provide valuable information for understanding the impact of environmentally relevant-level CeO2 NP exposure on soil microbial communities and metabolites with or without ferrous, which is needed to understand the ecological risk posed by long-term CeO2 NP exposure in rice-planted soil with rich ferrous.
32663007	1	16	theme	CeO2	227:230	arg1	NP					246:247	NP	246:247	NP	246:247	The environmental risks and benefits associated with the introduction of CeO2 nanoparticle (NP) in agricultural soil must be carefully assessed.
32663007	1	16	theme	CeO2	227:230	arg1	nanoparticle					232:243	CeO2 nanoparticle	227:243	CeO2 nanoparticle (NP)	227:248	The environmental risks and benefits associated with the introduction of CeO2 nanoparticle (NP) in agricultural soil must be carefully assessed.
32663007	4	17	theme	NP	674:675	arg1	exposure					677:684	CeO2 NP exposure	669:684	CeO2 NP exposure	669:684	Results showed that CeO2 NP exposure changed soil bacterial community compositions and soil metabolomics, and the above changes were further shifted with the ferrous amendment.
32663007	12	18	theme	palmitic	2117:2124	arg1	metabolites					2046:2056	The eight changed metabolites	2028:2056	The eight changed metabolites containing glycerol monstearate	2028:2088	The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure.
32663007	12	18	theme	palmitic	2117:2124	arg1	acid					2126:2129	palmitic acid	2117:2129	palmitic acid	2117:2129	The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure.
32663007	15	19	theme	NP	2785:2786	arg1	exposure					2788:2795	CeO2 NP exposure	2780:2795	CeO2 NP exposure	2780:2795	The presence of CeO2 NP mainly promoted fatty acids (hexanoic acid, nonanoic acid) and amino acid (oxoproline) and amine (diethanolamine) concentrations, which could be from the increased Proteobacteria abundance after CeO2 NP exposure.
32663007	3	20	theme	soil	564:567	arg1	communities					579:589	soil bacterial communities	564:589	soil bacterial communities	564:589	The aim of this study was to investigate the effects of environmentally relevant-level CeO2 NP (25 mg·kg-1) in the absence or presence of ferrous (30 mg·kg-1) amendment on soil bacterial communities and soil metabolomics in rice-planted soil over 150 days.
32663007	5	21	theme	Several	826:832	arg1	phyla					869:873	Several functionally significant bacterial phyla	826:873	Several functionally significant bacterial phyla	826:873	Several functionally significant bacterial phyla containing Proteobacteria and Bacteroidetes abundances, which were associated with carbon and nitrogen cycling, were promoted after CeO2 NP exposure with ferrous amendment.
32663007	13	22	contain	containing	2378:2387	arg1	metabolites					2366:2376	dominant metabolites	2357:2376	dominant metabolites containing fatty acids, inorganic acid, and sugar	2357:2426	Activities of soil enzymes (urease, invertase, and cellulose), pH, and soil organic matter affected dominant metabolites containing fatty acids, inorganic acid, and sugar.
32663007	13	22	contain	containing	2378:2387	arg2	acid					2412:2415	inorganic acid	2402:2415	inorganic acid	2402:2415	Activities of soil enzymes (urease, invertase, and cellulose), pH, and soil organic matter affected dominant metabolites containing fatty acids, inorganic acid, and sugar.
32663007	13	22	contain	containing	2378:2387	arg2	acids					2395:2399	fatty acids	2389:2399	fatty acids	2389:2399	Activities of soil enzymes (urease, invertase, and cellulose), pH, and soil organic matter affected dominant metabolites containing fatty acids, inorganic acid, and sugar.
32663007	13	22	contain	containing	2378:2387	arg2	sugar					2422:2426	sugar	2422:2426	sugar	2422:2426	Activities of soil enzymes (urease, invertase, and cellulose), pH, and soil organic matter affected dominant metabolites containing fatty acids, inorganic acid, and sugar.
32663007	8	23	theme	Firmicutes	1327:1336	arg1	abundances					1398:1407	Firmicutes and increased Chloroflexi, Rokubacteria, and Thaumarchaeota abundances	1327:1407	Firmicutes and increased Chloroflexi, Rokubacteria, and Thaumarchaeota abundances	1327:1407	NP decreased Firmicutes and increased Chloroflexi, Rokubacteria, and Thaumarchaeota abundances at the phylum level, which contributed to reduce soil cellulose activity.
32663007	16	24	theme	most	2828:2831	arg1	species					2839:2845	the most genus species	2824:2845	the most genus species containing 13 genera affecting soil metabolite profiles	2824:2901	Phylum Proteobacteria had the most genus species containing 13 genera affecting soil metabolite profiles.
32663007	3	25	theme	amendment	551:559	arg1	presence					518:525	presence	518:525	presence	518:525	The aim of this study was to investigate the effects of environmentally relevant-level CeO2 NP (25 mg·kg-1) in the absence or presence of ferrous (30 mg·kg-1) amendment on soil bacterial communities and soil metabolomics in rice-planted soil over 150 days.
32663007	3	25	theme	amendment	551:559	arg1	absence					507:513	absence	507:513	absence	507:513	The aim of this study was to investigate the effects of environmentally relevant-level CeO2 NP (25 mg·kg-1) in the absence or presence of ferrous (30 mg·kg-1) amendment on soil bacterial communities and soil metabolomics in rice-planted soil over 150 days.
32663007	13	26	theme	soil	2271:2274	arg1	invertase					2293:2301	invertase	2293:2301	invertase	2293:2301	Activities of soil enzymes (urease, invertase, and cellulose), pH, and soil organic matter affected dominant metabolites containing fatty acids, inorganic acid, and sugar.
32663007	13	26	theme	soil	2271:2274	arg1	cellulose					2308:2316	cellulose	2308:2316	cellulose	2308:2316	Activities of soil enzymes (urease, invertase, and cellulose), pH, and soil organic matter affected dominant metabolites containing fatty acids, inorganic acid, and sugar.
32663007	13	26	theme	soil	2271:2274	arg1	urease					2285:2290	urease	2285:2290	urease	2285:2290	Activities of soil enzymes (urease, invertase, and cellulose), pH, and soil organic matter affected dominant metabolites containing fatty acids, inorganic acid, and sugar.
32663007	13	26	theme	soil	2271:2274	arg1	enzymes					2276:2282	soil enzymes	2271:2282	soil enzymes (urease, invertase, and cellulose)	2271:2317	Activities of soil enzymes (urease, invertase, and cellulose), pH, and soil organic matter affected dominant metabolites containing fatty acids, inorganic acid, and sugar.
32663007	8	27	theme	increased	1342:1350	arg1	Chloroflexi					1352:1362	increased Chloroflexi	1342:1362	increased Chloroflexi	1342:1362	NP decreased Firmicutes and increased Chloroflexi, Rokubacteria, and Thaumarchaeota abundances at the phylum level, which contributed to reduce soil cellulose activity.
32663007	15	28	theme	Proteobacteria	2749:2762	arg1	abundance					2764:2772	the increased Proteobacteria abundance	2735:2772	the increased Proteobacteria abundance	2735:2772	The presence of CeO2 NP mainly promoted fatty acids (hexanoic acid, nonanoic acid) and amino acid (oxoproline) and amine (diethanolamine) concentrations, which could be from the increased Proteobacteria abundance after CeO2 NP exposure.
32663007	10	29	theme	phylum	1775:1780	arg1	level					1782:1786	the phylum level	1771:1786	the phylum level	1771:1786	As a result, the above factors were related to the changes of Chloroflexi, Gemmatimonadetes, Rokubacteria, Thaumarchaeota, and Nitrospirae at the phylum level.
32663007	9	30	theme	Ce	1548:1549	arg1	accumulation					1551:1562	Ce accumulation	1548:1562	Ce accumulation in root	1548:1570	Additionally, CeO2 NP positively or negatively affected soil pH, Ce accumulation in root, and rice physiological properties (root-POD, stem-POD).
32663007	4	31	theme	soil	694:697	arg1	compositions					719:730	soil bacterial community compositions	694:730	soil bacterial community compositions	694:730	Results showed that CeO2 NP exposure changed soil bacterial community compositions and soil metabolomics, and the above changes were further shifted with the ferrous amendment.
32663007	5	32	dep	carbon	958:963	arg1	cycling					978:984	cycling	978:984	cycling	978:984	Several functionally significant bacterial phyla containing Proteobacteria and Bacteroidetes abundances, which were associated with carbon and nitrogen cycling, were promoted after CeO2 NP exposure with ferrous amendment.
32663007	0	33	theme	Soil	69:72	arg1	Compositions					94:105	Soil Bacterial Community Compositions	69:105	Soil Bacterial Community Compositions	69:105	Environmentally Relevant-Level CeO2 NP with Ferrous Amendment Alters Soil Bacterial Community Compositions and Metabolite Profiles in Rice-Planted Soils.
32663007	5	34	contain	containing	875:884	arg2	Bacteroidetes					905:917	Bacteroidetes	905:917	Bacteroidetes	905:917	Several functionally significant bacterial phyla containing Proteobacteria and Bacteroidetes abundances, which were associated with carbon and nitrogen cycling, were promoted after CeO2 NP exposure with ferrous amendment.
32663007	5	34	contain	containing	875:884	arg2	Proteobacteria					886:899	Proteobacteria	886:899	Proteobacteria	886:899	Several functionally significant bacterial phyla containing Proteobacteria and Bacteroidetes abundances, which were associated with carbon and nitrogen cycling, were promoted after CeO2 NP exposure with ferrous amendment.
32663007	5	34	contain	containing	875:884	arg1	phyla					869:873	Several functionally significant bacterial phyla	826:873	Several functionally significant bacterial phyla	826:873	Several functionally significant bacterial phyla containing Proteobacteria and Bacteroidetes abundances, which were associated with carbon and nitrogen cycling, were promoted after CeO2 NP exposure with ferrous amendment.
32663007	4	35	theme	CeO2	669:672	arg1	exposure					677:684	CeO2 NP exposure	669:684	CeO2 NP exposure	669:684	Results showed that CeO2 NP exposure changed soil bacterial community compositions and soil metabolomics, and the above changes were further shifted with the ferrous amendment.
32663007	17	36	theme	CeO2	3167:3170	arg1	exposure					3175:3182	long-term CeO2 NP exposure	3157:3182	long-term CeO2 NP exposure	3157:3182	These results provide valuable information for understanding the impact of environmentally relevant-level CeO2 NP exposure on soil microbial communities and metabolites with or without ferrous, which is needed to understand the ecological risk posed by long-term CeO2 NP exposure in rice-planted soil with rich ferrous.
32663007	0	37	theme	Community	84:92	arg1	Compositions					94:105	Soil Bacterial Community Compositions	69:105	Soil Bacterial Community Compositions	69:105	Environmentally Relevant-Level CeO2 NP with Ferrous Amendment Alters Soil Bacterial Community Compositions and Metabolite Profiles in Rice-Planted Soils.
32663007	3	38	theme	soil	595:598	arg1	metabolomics					600:611	soil metabolomics	595:611	soil metabolomics	595:611	The aim of this study was to investigate the effects of environmentally relevant-level CeO2 NP (25 mg·kg-1) in the absence or presence of ferrous (30 mg·kg-1) amendment on soil bacterial communities and soil metabolomics in rice-planted soil over 150 days.
32663007	8	39	theme	Thaumarchaeota	1383:1396	arg1	abundances					1398:1407	Firmicutes and increased Chloroflexi, Rokubacteria, and Thaumarchaeota abundances	1327:1407	Firmicutes and increased Chloroflexi, Rokubacteria, and Thaumarchaeota abundances	1327:1407	NP decreased Firmicutes and increased Chloroflexi, Rokubacteria, and Thaumarchaeota abundances at the phylum level, which contributed to reduce soil cellulose activity.
32663007	5	40	dep	Proteobacteria	886:899	arg1	abundances					919:928	abundances	919:928	abundances	919:928	Several functionally significant bacterial phyla containing Proteobacteria and Bacteroidetes abundances, which were associated with carbon and nitrogen cycling, were promoted after CeO2 NP exposure with ferrous amendment.
32663007	15	41	theme	nonanoic	2629:2636	arg1	acid					2623:2626	hexanoic acid	2614:2626	hexanoic acid	2614:2626	The presence of CeO2 NP mainly promoted fatty acids (hexanoic acid, nonanoic acid) and amino acid (oxoproline) and amine (diethanolamine) concentrations, which could be from the increased Proteobacteria abundance after CeO2 NP exposure.
32663007	15	41	theme	nonanoic	2629:2636	arg1	acid					2638:2641	nonanoic acid	2629:2641	nonanoic acid	2629:2641	The presence of CeO2 NP mainly promoted fatty acids (hexanoic acid, nonanoic acid) and amino acid (oxoproline) and amine (diethanolamine) concentrations, which could be from the increased Proteobacteria abundance after CeO2 NP exposure.
32663007	9	42	theme	rice	1577:1580	arg1	properties					1596:1605	rice physiological properties	1577:1605	rice physiological properties (root-POD, stem-POD)	1577:1626	Additionally, CeO2 NP positively or negatively affected soil pH, Ce accumulation in root, and rice physiological properties (root-POD, stem-POD).
32663007	12	43	theme	NP	2244:2245	arg1	exposure					2247:2254	CeO2 NP exposure	2239:2254	CeO2 NP exposure	2239:2254	The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure.
32663007	12	44	theme	CeO2	2239:2242	arg1	exposure					2247:2254	CeO2 NP exposure	2239:2254	CeO2 NP exposure	2239:2254	The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure.
32663007	11	45	theme	sucrose	1906:1912	arg1	metabolism					1914:1923	starch and sucrose metabolism	1895:1923	metabolism	1914:1923	After adding CeO2 NP with ferrous or not, the main metabolic changes were concentrated on fluctuations in starch and sucrose metabolism, nitrogen metabolism, sulfur metabolism, propanoate metabolism, fatty acid metabolism, and urea cycle.
32663007	7	46	theme	enzyme	1231:1236	arg1	activities					1238:1247	rhizosphere soil enzyme activities	1214:1247	rhizosphere soil enzyme activities	1214:1247	Among rhizosphere soil enzyme activities, cellulose activity was the most sensitive for CeO2 NP exposure.
32663007	6	47	theme	plant-growth-promoting	1075:1096	arg1	rhizobacteria					1098:1110	plant-growth-promoting rhizobacteria	1075:1110	plant-growth-promoting rhizobacteria containing genera Bacillus and Arthrobacter	1075:1154	However, CeO2 NP inhibited plant-growth-promoting rhizobacteria containing genera Bacillus and Arthrobacter irrespective of the presence or absence of ferrous.
32663007	4	48	theme	community	709:717	arg1	compositions					719:730	soil bacterial community compositions	694:730	soil bacterial community compositions	694:730	Results showed that CeO2 NP exposure changed soil bacterial community compositions and soil metabolomics, and the above changes were further shifted with the ferrous amendment.
32663007	11	49	theme	starch	1895:1900	arg1	metabolism					1914:1923	starch and sucrose metabolism	1895:1923	metabolism	1914:1923	After adding CeO2 NP with ferrous or not, the main metabolic changes were concentrated on fluctuations in starch and sucrose metabolism, nitrogen metabolism, sulfur metabolism, propanoate metabolism, fatty acid metabolism, and urea cycle.
32663007	0	50	theme	Environmentally	0:14	arg1	CeO2					31:34	Environmentally Relevant-Level CeO2 NP	0:37	Environmentally Relevant-Level CeO2 NP with Ferrous Amendment	0:60	Environmentally Relevant-Level CeO2 NP with Ferrous Amendment Alters Soil Bacterial Community Compositions and Metabolite Profiles in Rice-Planted Soils.
32663007	17	51	with	soil	3200:3203	arg1	ferrous					3215:3221	ferrous	3215:3221	ferrous	3215:3221	These results provide valuable information for understanding the impact of environmentally relevant-level CeO2 NP exposure on soil microbial communities and metabolites with or without ferrous, which is needed to understand the ecological risk posed by long-term CeO2 NP exposure in rice-planted soil with rich ferrous.
32663007	12	52	theme	stearic	2168:2174	arg1	acid					2176:2179	stearic acid	2168:2179	stearic acid	2168:2179	The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure.
32663007	12	52	theme	stearic	2168:2174	arg1	metabolites					2046:2056	The eight changed metabolites	2028:2056	The eight changed metabolites containing glycerol monstearate	2028:2088	The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure.
32663007	10	53	theme	Chloroflexi	1691:1701	arg1	changes					1680:1686	the changes	1676:1686	the changes of Chloroflexi, Gemmatimonadetes, Rokubacteria, Thaumarchaeota, and Nitrospirae	1676:1766	As a result, the above factors were related to the changes of Chloroflexi, Gemmatimonadetes, Rokubacteria, Thaumarchaeota, and Nitrospirae at the phylum level.
32663007	15	54	theme	amine	2676:2680	arg1	concentrations					2699:2712	amine (diethanolamine) concentrations	2676:2712	amine (diethanolamine) concentrations	2676:2712	The presence of CeO2 NP mainly promoted fatty acids (hexanoic acid, nonanoic acid) and amino acid (oxoproline) and amine (diethanolamine) concentrations, which could be from the increased Proteobacteria abundance after CeO2 NP exposure.
32663007	17	55	from	impact	2969:2974	arg1	metabolites					3061:3071	metabolites	3061:3071	metabolites	3061:3071	These results provide valuable information for understanding the impact of environmentally relevant-level CeO2 NP exposure on soil microbial communities and metabolites with or without ferrous, which is needed to understand the ecological risk posed by long-term CeO2 NP exposure in rice-planted soil with rich ferrous.
32663007	17	55	from	impact	2969:2974	arg1	communities					3045:3055	soil microbial communities	3030:3055	soil microbial communities	3030:3055	These results provide valuable information for understanding the impact of environmentally relevant-level CeO2 NP exposure on soil microbial communities and metabolites with or without ferrous, which is needed to understand the ecological risk posed by long-term CeO2 NP exposure in rice-planted soil with rich ferrous.
32663007	6	56	dep	genera	1123:1128	arg1	Bacillus					1130:1137	genera Bacillus	1123:1137	genera Bacillus	1123:1137	However, CeO2 NP inhibited plant-growth-promoting rhizobacteria containing genera Bacillus and Arthrobacter irrespective of the presence or absence of ferrous.
32663007	13	57	theme	matter	2341:2346	arg1	Activities					2257:2266	Activities	2257:2266	Activities of soil enzymes (urease, invertase, and cellulose), pH, and soil organic matter	2257:2346	Activities of soil enzymes (urease, invertase, and cellulose), pH, and soil organic matter affected dominant metabolites containing fatty acids, inorganic acid, and sugar.
32663007	15	58	theme	amino	2648:2652	arg1	oxoproline					2660:2669	oxoproline	2660:2669	oxoproline	2660:2669	The presence of CeO2 NP mainly promoted fatty acids (hexanoic acid, nonanoic acid) and amino acid (oxoproline) and amine (diethanolamine) concentrations, which could be from the increased Proteobacteria abundance after CeO2 NP exposure.
32663007	15	58	theme	amino	2648:2652	arg1	acid					2654:2657	amino acid	2648:2657	amino acid (oxoproline)	2648:2670	The presence of CeO2 NP mainly promoted fatty acids (hexanoic acid, nonanoic acid) and amino acid (oxoproline) and amine (diethanolamine) concentrations, which could be from the increased Proteobacteria abundance after CeO2 NP exposure.
32663007	0	59	with	CeO2	31:34	arg1	Amendment					52:60	Ferrous Amendment	44:60	Ferrous Amendment	44:60	Environmentally Relevant-Level CeO2 NP with Ferrous Amendment Alters Soil Bacterial Community Compositions and Metabolite Profiles in Rice-Planted Soils.
32663007	2	60	theme	rice	346:349	arg1	soil					338:341	rhizosphere soil	326:341	rhizosphere soil of rice due to the reduction states underground	326:389	The ferrous ion is rich in rhizosphere soil of rice due to the reduction states underground.
32663007	14	61	theme	bacterial	2480:2488	arg1	community					2490:2498	soil bacterial community	2475:2498	soil bacterial community	2475:2498	Network analysis showed that the influence of soil bacterial community on metabolites varied with metabolites and bacteria species.
32663007	13	62	theme	dominant	2357:2364	arg1	metabolites					2366:2376	dominant metabolites	2357:2376	dominant metabolites containing fatty acids, inorganic acid, and sugar	2357:2426	Activities of soil enzymes (urease, invertase, and cellulose), pH, and soil organic matter affected dominant metabolites containing fatty acids, inorganic acid, and sugar.
32663007	8	63	theme	cellulose	1463:1471	arg1	activity					1473:1480	soil cellulose activity	1458:1480	soil cellulose activity	1458:1480	NP decreased Firmicutes and increased Chloroflexi, Rokubacteria, and Thaumarchaeota abundances at the phylum level, which contributed to reduce soil cellulose activity.
32663007	3	64	theme	CeO2	479:482	arg1	NP					484:485	environmentally relevant-level CeO2 NP	448:485	environmentally relevant-level CeO2 NP (25 mg·kg-1) in the absence or presence of ferrous (30 mg·kg-1) amendment	448:559	The aim of this study was to investigate the effects of environmentally relevant-level CeO2 NP (25 mg·kg-1) in the absence or presence of ferrous (30 mg·kg-1) amendment on soil bacterial communities and soil metabolomics in rice-planted soil over 150 days.
32663007	3	64	theme	CeO2	479:482	arg1	mg·kg-1					491:497	25 mg·kg-1	488:497	25 mg·kg-1	488:497	The aim of this study was to investigate the effects of environmentally relevant-level CeO2 NP (25 mg·kg-1) in the absence or presence of ferrous (30 mg·kg-1) amendment on soil bacterial communities and soil metabolomics in rice-planted soil over 150 days.
32663007	17	65	theme	CeO2	3010:3013	arg1	exposure					3018:3025	environmentally relevant-level CeO2 NP exposure	2979:3025	environmentally relevant-level CeO2 NP exposure	2979:3025	These results provide valuable information for understanding the impact of environmentally relevant-level CeO2 NP exposure on soil microbial communities and metabolites with or without ferrous, which is needed to understand the ecological risk posed by long-term CeO2 NP exposure in rice-planted soil with rich ferrous.
32663007	12	66	theme	changed	2038:2044	arg1	alkane					2132:2137	alkane	2132:2137	alkane	2132:2137	The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure.
32663007	12	66	theme	changed	2038:2044	arg1	metabolites					2046:2056	The eight changed metabolites	2028:2056	The eight changed metabolites containing glycerol monstearate	2028:2088	The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure.
32663007	12	66	theme	changed	2038:2044	arg1	acid					2176:2179	stearic acid	2168:2179	stearic acid	2168:2179	The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure.
32663007	12	66	theme	changed	2038:2044	arg1	monopalmitin					2103:2114	monopalmitin	2103:2114	monopalmitin	2103:2114	The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure.
32663007	12	66	theme	changed	2038:2044	arg1	ethanol					2140:2146	ethanol	2140:2146	ethanol	2140:2146	The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure.
32663007	12	66	theme	changed	2038:2044	arg1	dicarboximide					2149:2161	dicarboximide	2149:2161	dicarboximide	2149:2161	The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure.
32663007	12	66	theme	changed	2038:2044	arg1	acid					2126:2129	palmitic acid	2117:2129	palmitic acid	2117:2129	The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure.
32663007	12	66	theme	changed	2038:2044	arg1	acid					2097:2100	boric acid	2091:2100	boric acid	2091:2100	The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure.
32663007	17	67	theme	ecological	3132:3141	arg1	risk					3143:3146	the ecological risk	3128:3146	the ecological risk posed by long-term CeO2 NP exposure in rice-planted soil with rich ferrous	3128:3221	These results provide valuable information for understanding the impact of environmentally relevant-level CeO2 NP exposure on soil microbial communities and metabolites with or without ferrous, which is needed to understand the ecological risk posed by long-term CeO2 NP exposure in rice-planted soil with rich ferrous.
32663007	2	68	theme	reduction	362:370	arg1	underground					379:389	the reduction states underground	358:389	the reduction states underground	358:389	The ferrous ion is rich in rhizosphere soil of rice due to the reduction states underground.
32663007	5	69	theme	NP	1012:1013	arg1	exposure					1015:1022	CeO2 NP exposure	1007:1022	CeO2 NP exposure with ferrous amendment	1007:1045	Several functionally significant bacterial phyla containing Proteobacteria and Bacteroidetes abundances, which were associated with carbon and nitrogen cycling, were promoted after CeO2 NP exposure with ferrous amendment.
32663007	7	70	theme	CeO2	1296:1299	arg1	exposure					1304:1311	CeO2 NP exposure	1296:1311	CeO2 NP exposure	1296:1311	Among rhizosphere soil enzyme activities, cellulose activity was the most sensitive for CeO2 NP exposure.
32663007	2	71	from	soil	338:341	arg1	rich					318:321	rich	318:321	rich	318:321	The ferrous ion is rich in rhizosphere soil of rice due to the reduction states underground.
32663007	2	71	from	soil	338:341	arg1	ion					311:313	The ferrous ion	299:313	The ferrous ion	299:313	The ferrous ion is rich in rhizosphere soil of rice due to the reduction states underground.
32663007	16	72	theme	soil	2878:2881	arg1	profiles					2894:2901	soil metabolite profiles	2878:2901	soil metabolite profiles	2878:2901	Phylum Proteobacteria had the most genus species containing 13 genera affecting soil metabolite profiles.
32663007	2	73	theme	ferrous	303:309	arg1	rich					318:321	rich	318:321	rich	318:321	The ferrous ion is rich in rhizosphere soil of rice due to the reduction states underground.
32663007	2	73	theme	ferrous	303:309	arg1	ion					311:313	The ferrous ion	299:313	The ferrous ion	299:313	The ferrous ion is rich in rhizosphere soil of rice due to the reduction states underground.
32663007	14	74	theme	Network	2429:2435	arg1	analysis					2437:2444	Network analysis	2429:2444	Network analysis	2429:2444	Network analysis showed that the influence of soil bacterial community on metabolites varied with metabolites and bacteria species.
32663007	11	75	theme	metabolic	1840:1848	arg1	changes					1850:1856	the main metabolic changes	1831:1856	the main metabolic changes	1831:1856	After adding CeO2 NP with ferrous or not, the main metabolic changes were concentrated on fluctuations in starch and sucrose metabolism, nitrogen metabolism, sulfur metabolism, propanoate metabolism, fatty acid metabolism, and urea cycle.
32663007	9	76	from	accumulation	1551:1562	arg1	root					1567:1570	root	1567:1570	root	1567:1570	Additionally, CeO2 NP positively or negatively affected soil pH, Ce accumulation in root, and rice physiological properties (root-POD, stem-POD).
32663007	12	77	theme	glycerol	2069:2076	arg1	monstearate					2078:2088	glycerol monstearate	2069:2088	glycerol monstearate	2069:2088	The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure.
32663007	5	78	with	exposure	1015:1022	arg1	amendment					1037:1045	ferrous amendment	1029:1045	ferrous amendment	1029:1045	Several functionally significant bacterial phyla containing Proteobacteria and Bacteroidetes abundances, which were associated with carbon and nitrogen cycling, were promoted after CeO2 NP exposure with ferrous amendment.
32663007	10	79	from	level	1782:1786	arg1	related					1665:1671	related	1665:1671	related	1665:1671	As a result, the above factors were related to the changes of Chloroflexi, Gemmatimonadetes, Rokubacteria, Thaumarchaeota, and Nitrospirae at the phylum level.
32663007	10	79	from	level	1782:1786	arg1	factors					1652:1658	the above factors	1642:1658	the above factors	1642:1658	As a result, the above factors were related to the changes of Chloroflexi, Gemmatimonadetes, Rokubacteria, Thaumarchaeota, and Nitrospirae at the phylum level.
32663007	17	80	theme	relevant-level	2995:3008	arg1	exposure					3018:3025	environmentally relevant-level CeO2 NP exposure	2979:3025	environmentally relevant-level CeO2 NP exposure	2979:3025	These results provide valuable information for understanding the impact of environmentally relevant-level CeO2 NP exposure on soil microbial communities and metabolites with or without ferrous, which is needed to understand the ecological risk posed by long-term CeO2 NP exposure in rice-planted soil with rich ferrous.
32663007	5	81	theme	bacterial	859:867	arg1	phyla					869:873	Several functionally significant bacterial phyla	826:873	Several functionally significant bacterial phyla	826:873	Several functionally significant bacterial phyla containing Proteobacteria and Bacteroidetes abundances, which were associated with carbon and nitrogen cycling, were promoted after CeO2 NP exposure with ferrous amendment.
32663007	4	82	theme	ferrous	807:813	arg1	amendment					815:823	the ferrous amendment	803:823	the ferrous amendment	803:823	Results showed that CeO2 NP exposure changed soil bacterial community compositions and soil metabolomics, and the above changes were further shifted with the ferrous amendment.
32663007	6	83	theme	ferrous	1199:1205	arg1	absence					1188:1194	absence	1188:1194	absence	1188:1194	However, CeO2 NP inhibited plant-growth-promoting rhizobacteria containing genera Bacillus and Arthrobacter irrespective of the presence or absence of ferrous.
32663007	6	83	theme	ferrous	1199:1205	arg1	presence					1176:1183	presence	1176:1183	presence	1176:1183	However, CeO2 NP inhibited plant-growth-promoting rhizobacteria containing genera Bacillus and Arthrobacter irrespective of the presence or absence of ferrous.
32663007	6	84	dep	presence	1176:1183	arg1	the					1172:1174	the	1172:1174	the	1172:1174	However, CeO2 NP inhibited plant-growth-promoting rhizobacteria containing genera Bacillus and Arthrobacter irrespective of the presence or absence of ferrous.
32663007	3	85	from	NP	484:485	arg1	presence					518:525	presence	518:525	presence	518:525	The aim of this study was to investigate the effects of environmentally relevant-level CeO2 NP (25 mg·kg-1) in the absence or presence of ferrous (30 mg·kg-1) amendment on soil bacterial communities and soil metabolomics in rice-planted soil over 150 days.
32663007	3	85	from	NP	484:485	arg1	absence					507:513	absence	507:513	absence	507:513	The aim of this study was to investigate the effects of environmentally relevant-level CeO2 NP (25 mg·kg-1) in the absence or presence of ferrous (30 mg·kg-1) amendment on soil bacterial communities and soil metabolomics in rice-planted soil over 150 days.
32663007	17	86	theme	NP	3015:3016	arg1	exposure					3018:3025	environmentally relevant-level CeO2 NP exposure	2979:3025	environmentally relevant-level CeO2 NP exposure	2979:3025	These results provide valuable information for understanding the impact of environmentally relevant-level CeO2 NP exposure on soil microbial communities and metabolites with or without ferrous, which is needed to understand the ecological risk posed by long-term CeO2 NP exposure in rice-planted soil with rich ferrous.
32663007	15	87	theme	fatty	2601:2605	arg1	acids					2607:2611	fatty acids	2601:2611	fatty acids (hexanoic acid, nonanoic acid)	2601:2642	The presence of CeO2 NP mainly promoted fatty acids (hexanoic acid, nonanoic acid) and amino acid (oxoproline) and amine (diethanolamine) concentrations, which could be from the increased Proteobacteria abundance after CeO2 NP exposure.
32663007	9	88	theme	CeO2	1497:1500	arg1	NP					1502:1503	CeO2 NP	1497:1503	CeO2 NP	1497:1503	Additionally, CeO2 NP positively or negatively affected soil pH, Ce accumulation in root, and rice physiological properties (root-POD, stem-POD).
32663007	16	89	contain	had	2820:2822	arg2	species					2839:2845	the most genus species	2824:2845	the most genus species containing 13 genera affecting soil metabolite profiles	2824:2901	Phylum Proteobacteria had the most genus species containing 13 genera affecting soil metabolite profiles.
32663007	16	89	contain	had	2820:2822	arg1	Proteobacteria					2805:2818	Phylum Proteobacteria	2798:2818	Phylum Proteobacteria	2798:2818	Phylum Proteobacteria had the most genus species containing 13 genera affecting soil metabolite profiles.
32663007	15	90	theme	diethanolamine	2683:2696	arg1	concentrations					2699:2712	amine (diethanolamine) concentrations	2676:2712	amine (diethanolamine) concentrations	2676:2712	The presence of CeO2 NP mainly promoted fatty acids (hexanoic acid, nonanoic acid) and amino acid (oxoproline) and amine (diethanolamine) concentrations, which could be from the increased Proteobacteria abundance after CeO2 NP exposure.
32663007	3	91	theme	ferrous	530:536	arg1	amendment					551:559	ferrous (30 mg·kg-1) amendment	530:559	ferrous (30 mg·kg-1) amendment	530:559	The aim of this study was to investigate the effects of environmentally relevant-level CeO2 NP (25 mg·kg-1) in the absence or presence of ferrous (30 mg·kg-1) amendment on soil bacterial communities and soil metabolomics in rice-planted soil over 150 days.
32663007	3	91	theme	ferrous	530:536	arg1	mg·kg-1					542:548	30 mg·kg-1	539:548	30 mg·kg-1	539:548	The aim of this study was to investigate the effects of environmentally relevant-level CeO2 NP (25 mg·kg-1) in the absence or presence of ferrous (30 mg·kg-1) amendment on soil bacterial communities and soil metabolomics in rice-planted soil over 150 days.
32663007	13	92	theme	inorganic	2402:2410	arg1	acid					2412:2415	inorganic acid	2402:2415	inorganic acid	2402:2415	Activities of soil enzymes (urease, invertase, and cellulose), pH, and soil organic matter affected dominant metabolites containing fatty acids, inorganic acid, and sugar.
32663007	10	93	theme	above	1646:1650	arg1	related					1665:1671	related	1665:1671	related	1665:1671	As a result, the above factors were related to the changes of Chloroflexi, Gemmatimonadetes, Rokubacteria, Thaumarchaeota, and Nitrospirae at the phylum level.
32663007	10	93	theme	above	1646:1650	arg1	factors					1652:1658	the above factors	1642:1658	the above factors	1642:1658	As a result, the above factors were related to the changes of Chloroflexi, Gemmatimonadetes, Rokubacteria, Thaumarchaeota, and Nitrospirae at the phylum level.
32663007	15	94	theme	CeO2	2780:2783	arg1	exposure					2788:2795	CeO2 NP exposure	2780:2795	CeO2 NP exposure	2780:2795	The presence of CeO2 NP mainly promoted fatty acids (hexanoic acid, nonanoic acid) and amino acid (oxoproline) and amine (diethanolamine) concentrations, which could be from the increased Proteobacteria abundance after CeO2 NP exposure.
32663007	15	95	theme	CeO2	2577:2580	arg1	NP					2582:2583	CeO2 NP	2577:2583	CeO2 NP	2577:2583	The presence of CeO2 NP mainly promoted fatty acids (hexanoic acid, nonanoic acid) and amino acid (oxoproline) and amine (diethanolamine) concentrations, which could be from the increased Proteobacteria abundance after CeO2 NP exposure.
32663007	1	96	theme	nanoparticle	232:243	arg1	introduction					211:222	the introduction	207:222	the introduction of CeO2 nanoparticle (NP) in agricultural soil	207:269	The environmental risks and benefits associated with the introduction of CeO2 nanoparticle (NP) in agricultural soil must be carefully assessed.
32663007	16	97	theme	genus	2833:2837	arg1	species					2839:2845	the most genus species	2824:2845	the most genus species containing 13 genera affecting soil metabolite profiles	2824:2901	Phylum Proteobacteria had the most genus species containing 13 genera affecting soil metabolite profiles.
32663007	11	98	theme	sulfur	1947:1952	arg1	metabolism					1954:1963	sulfur metabolism	1947:1963	sulfur metabolism	1947:1963	After adding CeO2 NP with ferrous or not, the main metabolic changes were concentrated on fluctuations in starch and sucrose metabolism, nitrogen metabolism, sulfur metabolism, propanoate metabolism, fatty acid metabolism, and urea cycle.
32663007	3	99	dep	absence	507:513	arg1	the					503:505	the	503:505	the	503:505	The aim of this study was to investigate the effects of environmentally relevant-level CeO2 NP (25 mg·kg-1) in the absence or presence of ferrous (30 mg·kg-1) amendment on soil bacterial communities and soil metabolomics in rice-planted soil over 150 days.
32663007	6	100	contain	containing	1112:1121	arg2	Arthrobacter					1143:1154	Arthrobacter	1143:1154	Arthrobacter	1143:1154	However, CeO2 NP inhibited plant-growth-promoting rhizobacteria containing genera Bacillus and Arthrobacter irrespective of the presence or absence of ferrous.
32663007	6	100	contain	containing	1112:1121	arg2	genera					1123:1128	genera Bacillus	1123:1137	genera Bacillus	1123:1137	However, CeO2 NP inhibited plant-growth-promoting rhizobacteria containing genera Bacillus and Arthrobacter irrespective of the presence or absence of ferrous.
32663007	6	100	contain	containing	1112:1121	arg1	rhizobacteria					1098:1110	plant-growth-promoting rhizobacteria	1075:1110	plant-growth-promoting rhizobacteria containing genera Bacillus and Arthrobacter	1075:1154	However, CeO2 NP inhibited plant-growth-promoting rhizobacteria containing genera Bacillus and Arthrobacter irrespective of the presence or absence of ferrous.
32663007	9	101	dep	properties	1596:1605	arg1	root-POD					1608:1615	root-POD	1608:1615	root-POD	1608:1615	Additionally, CeO2 NP positively or negatively affected soil pH, Ce accumulation in root, and rice physiological properties (root-POD, stem-POD).
32663007	9	101	dep	properties	1596:1605	arg1	stem-POD					1618:1625	stem-POD	1618:1625	stem-POD	1618:1625	Additionally, CeO2 NP positively or negatively affected soil pH, Ce accumulation in root, and rice physiological properties (root-POD, stem-POD).
32663007	15	102	theme	increased	2739:2747	arg1	abundance					2764:2772	the increased Proteobacteria abundance	2735:2772	the increased Proteobacteria abundance	2735:2772	The presence of CeO2 NP mainly promoted fatty acids (hexanoic acid, nonanoic acid) and amino acid (oxoproline) and amine (diethanolamine) concentrations, which could be from the increased Proteobacteria abundance after CeO2 NP exposure.
32663007	4	103	theme	above	763:767	arg1	changes					769:775	the above changes	759:775	the above changes	759:775	Results showed that CeO2 NP exposure changed soil bacterial community compositions and soil metabolomics, and the above changes were further shifted with the ferrous amendment.
32663007	11	104	theme	fatty	1989:1993	arg1	metabolism					2000:2009	fatty acid metabolism	1989:2009	fatty acid metabolism	1989:2009	After adding CeO2 NP with ferrous or not, the main metabolic changes were concentrated on fluctuations in starch and sucrose metabolism, nitrogen metabolism, sulfur metabolism, propanoate metabolism, fatty acid metabolism, and urea cycle.
32663007	13	105	theme	enzymes	2276:2282	arg1	Activities					2257:2266	Activities	2257:2266	Activities of soil enzymes (urease, invertase, and cellulose), pH, and soil organic matter	2257:2346	Activities of soil enzymes (urease, invertase, and cellulose), pH, and soil organic matter affected dominant metabolites containing fatty acids, inorganic acid, and sugar.
32663007	12	106	theme	treatments	2223:2232	arg1	separation					2199:2208	the separation	2195:2208	the separation of different treatments with CeO2 NP exposure	2195:2254	The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure.
32663007	12	107	theme	boric	2091:2095	arg1	metabolites					2046:2056	The eight changed metabolites	2028:2056	The eight changed metabolites containing glycerol monstearate	2028:2088	The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure.
32663007	12	107	theme	boric	2091:2095	arg1	acid					2097:2100	boric acid	2091:2100	boric acid	2091:2100	The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure.
32663007	12	108	with	treatments	2223:2232	arg1	exposure					2247:2254	CeO2 NP exposure	2239:2254	CeO2 NP exposure	2239:2254	The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure.
32663007	11	109	theme	main	1835:1838	arg1	changes					1850:1856	the main metabolic changes	1831:1856	the main metabolic changes	1831:1856	After adding CeO2 NP with ferrous or not, the main metabolic changes were concentrated on fluctuations in starch and sucrose metabolism, nitrogen metabolism, sulfur metabolism, propanoate metabolism, fatty acid metabolism, and urea cycle.
32663007	0	110	theme	Bacterial	74:82	arg1	Compositions					94:105	Soil Bacterial Community Compositions	69:105	Soil Bacterial Community Compositions	69:105	Environmentally Relevant-Level CeO2 NP with Ferrous Amendment Alters Soil Bacterial Community Compositions and Metabolite Profiles in Rice-Planted Soils.
32663007	3	111	theme	bacterial	569:577	arg1	communities					579:589	soil bacterial communities	564:589	soil bacterial communities	564:589	The aim of this study was to investigate the effects of environmentally relevant-level CeO2 NP (25 mg·kg-1) in the absence or presence of ferrous (30 mg·kg-1) amendment on soil bacterial communities and soil metabolomics in rice-planted soil over 150 days.
32663007	1	112	from	introduction	211:222	arg1	soil					266:269	agricultural soil	253:269	agricultural soil	253:269	The environmental risks and benefits associated with the introduction of CeO2 nanoparticle (NP) in agricultural soil must be carefully assessed.
32663007	1	113	theme	environmental	158:170	arg1	risks					172:176	risks	172:176	risks	172:176	The environmental risks and benefits associated with the introduction of CeO2 nanoparticle (NP) in agricultural soil must be carefully assessed.
32663007	8	114	theme	Chloroflexi	1352:1362	arg1	abundances					1398:1407	Firmicutes and increased Chloroflexi, Rokubacteria, and Thaumarchaeota abundances	1327:1407	Firmicutes and increased Chloroflexi, Rokubacteria, and Thaumarchaeota abundances	1327:1407	NP decreased Firmicutes and increased Chloroflexi, Rokubacteria, and Thaumarchaeota abundances at the phylum level, which contributed to reduce soil cellulose activity.
32663007	3	115	from	absence	507:513	arg1	effects					437:443	the effects	433:443	the effects of environmentally relevant-level CeO2 NP (25 mg·kg-1) in the absence or presence of ferrous (30 mg·kg-1) amendment on soil bacterial communities and soil metabolomics	433:611	The aim of this study was to investigate the effects of environmentally relevant-level CeO2 NP (25 mg·kg-1) in the absence or presence of ferrous (30 mg·kg-1) amendment on soil bacterial communities and soil metabolomics in rice-planted soil over 150 days.
32663007	17	116	theme	long-term	3157:3165	arg1	exposure					3175:3182	long-term CeO2 NP exposure	3157:3182	long-term CeO2 NP exposure	3157:3182	These results provide valuable information for understanding the impact of environmentally relevant-level CeO2 NP exposure on soil microbial communities and metabolites with or without ferrous, which is needed to understand the ecological risk posed by long-term CeO2 NP exposure in rice-planted soil with rich ferrous.
32663007	7	117	theme	cellulose	1250:1258	arg1	activity					1260:1267	cellulose activity	1250:1267	cellulose activity	1250:1267	Among rhizosphere soil enzyme activities, cellulose activity was the most sensitive for CeO2 NP exposure.
32663007	7	117	theme	cellulose	1250:1258	arg1	sensitive					1282:1290	sensitive	1282:1290	sensitive	1282:1290	Among rhizosphere soil enzyme activities, cellulose activity was the most sensitive for CeO2 NP exposure.
32663007	9	118	from	properties	1596:1605	arg1	root					1567:1570	root	1567:1570	root	1567:1570	Additionally, CeO2 NP positively or negatively affected soil pH, Ce accumulation in root, and rice physiological properties (root-POD, stem-POD).
32663007	16	119	theme	Phylum	2798:2803	arg1	Proteobacteria					2805:2818	Phylum Proteobacteria	2798:2818	Phylum Proteobacteria	2798:2818	Phylum Proteobacteria had the most genus species containing 13 genera affecting soil metabolite profiles.
32663007	8	120	theme	Rokubacteria	1365:1376	arg1	abundances					1398:1407	Firmicutes and increased Chloroflexi, Rokubacteria, and Thaumarchaeota abundances	1327:1407	Firmicutes and increased Chloroflexi, Rokubacteria, and Thaumarchaeota abundances	1327:1407	NP decreased Firmicutes and increased Chloroflexi, Rokubacteria, and Thaumarchaeota abundances at the phylum level, which contributed to reduce soil cellulose activity.
32663007	3	121	from	presence	518:525	arg1	effects					437:443	the effects	433:443	the effects of environmentally relevant-level CeO2 NP (25 mg·kg-1) in the absence or presence of ferrous (30 mg·kg-1) amendment on soil bacterial communities and soil metabolomics	433:611	The aim of this study was to investigate the effects of environmentally relevant-level CeO2 NP (25 mg·kg-1) in the absence or presence of ferrous (30 mg·kg-1) amendment on soil bacterial communities and soil metabolomics in rice-planted soil over 150 days.
32663007	17	122	theme	NP	3172:3173	arg1	exposure					3175:3182	long-term CeO2 NP exposure	3157:3182	long-term CeO2 NP exposure	3157:3182	These results provide valuable information for understanding the impact of environmentally relevant-level CeO2 NP exposure on soil microbial communities and metabolites with or without ferrous, which is needed to understand the ecological risk posed by long-term CeO2 NP exposure in rice-planted soil with rich ferrous.
32663007	0	123	theme	Metabolite	111:120	arg1	Profiles					122:129	Metabolite Profiles	111:129	Metabolite Profiles	111:129	Environmentally Relevant-Level CeO2 NP with Ferrous Amendment Alters Soil Bacterial Community Compositions and Metabolite Profiles in Rice-Planted Soils.
32663007	2	124	from	rich	318:321	arg1	soil					338:341	rhizosphere soil	326:341	rhizosphere soil of rice due to the reduction states underground	326:389	The ferrous ion is rich in rhizosphere soil of rice due to the reduction states underground.
32663007	12	125	theme	different	2213:2221	arg1	treatments					2223:2232	different treatments	2213:2232	different treatments with CeO2 NP exposure	2213:2254	The eight changed metabolites containing glycerol monstearate, boric acid, monopalmitin, palmitic acid, alkane, ethanol, dicarboximide, and stearic acid accounted for the separation of different treatments with CeO2 NP exposure.
32663007	11	126	from	fluctuations	1879:1890	arg1	metabolism					1935:1944	nitrogen metabolism	1926:1944	nitrogen metabolism	1926:1944	After adding CeO2 NP with ferrous or not, the main metabolic changes were concentrated on fluctuations in starch and sucrose metabolism, nitrogen metabolism, sulfur metabolism, propanoate metabolism, fatty acid metabolism, and urea cycle.
32663007	11	126	from	fluctuations	1879:1890	arg1	metabolism					1977:1986	propanoate metabolism	1966:1986	propanoate metabolism	1966:1986	After adding CeO2 NP with ferrous or not, the main metabolic changes were concentrated on fluctuations in starch and sucrose metabolism, nitrogen metabolism, sulfur metabolism, propanoate metabolism, fatty acid metabolism, and urea cycle.
32663007	11	126	from	fluctuations	1879:1890	arg1	cycle					2021:2025	urea cycle	2016:2025	urea cycle	2016:2025	After adding CeO2 NP with ferrous or not, the main metabolic changes were concentrated on fluctuations in starch and sucrose metabolism, nitrogen metabolism, sulfur metabolism, propanoate metabolism, fatty acid metabolism, and urea cycle.
32663007	11	126	from	fluctuations	1879:1890	arg1	metabolism					1914:1923	starch and sucrose metabolism	1895:1923	metabolism	1914:1923	After adding CeO2 NP with ferrous or not, the main metabolic changes were concentrated on fluctuations in starch and sucrose metabolism, nitrogen metabolism, sulfur metabolism, propanoate metabolism, fatty acid metabolism, and urea cycle.
32663007	11	126	from	fluctuations	1879:1890	arg1	metabolism					1954:1963	sulfur metabolism	1947:1963	sulfur metabolism	1947:1963	After adding CeO2 NP with ferrous or not, the main metabolic changes were concentrated on fluctuations in starch and sucrose metabolism, nitrogen metabolism, sulfur metabolism, propanoate metabolism, fatty acid metabolism, and urea cycle.
32663007	11	126	from	fluctuations	1879:1890	arg1	metabolism					2000:2009	fatty acid metabolism	1989:2009	fatty acid metabolism	1989:2009	After adding CeO2 NP with ferrous or not, the main metabolic changes were concentrated on fluctuations in starch and sucrose metabolism, nitrogen metabolism, sulfur metabolism, propanoate metabolism, fatty acid metabolism, and urea cycle.
32663007	4	127	theme	soil	736:739	arg1	metabolomics					741:752	soil metabolomics	736:752	soil metabolomics	736:752	Results showed that CeO2 NP exposure changed soil bacterial community compositions and soil metabolomics, and the above changes were further shifted with the ferrous amendment.
32663007	2	128	theme	rhizosphere	326:336	arg1	soil					338:341	rhizosphere soil	326:341	rhizosphere soil of rice due to the reduction states underground	326:389	The ferrous ion is rich in rhizosphere soil of rice due to the reduction states underground.
32663007	7	129	theme	soil	1226:1229	arg1	activities					1238:1247	rhizosphere soil enzyme activities	1214:1247	rhizosphere soil enzyme activities	1214:1247	Among rhizosphere soil enzyme activities, cellulose activity was the most sensitive for CeO2 NP exposure.
32663007	9	130	theme	physiological	1582:1594	arg1	properties					1596:1605	rice physiological properties	1577:1605	rice physiological properties (root-POD, stem-POD)	1577:1626	Additionally, CeO2 NP positively or negatively affected soil pH, Ce accumulation in root, and rice physiological properties (root-POD, stem-POD).
32663007	15	131	theme	hexanoic	2614:2621	arg1	acid					2623:2626	hexanoic acid	2614:2626	hexanoic acid	2614:2626	The presence of CeO2 NP mainly promoted fatty acids (hexanoic acid, nonanoic acid) and amino acid (oxoproline) and amine (diethanolamine) concentrations, which could be from the increased Proteobacteria abundance after CeO2 NP exposure.
32663007	15	131	theme	hexanoic	2614:2621	arg1	acid					2638:2641	nonanoic acid	2629:2641	nonanoic acid	2629:2641	The presence of CeO2 NP mainly promoted fatty acids (hexanoic acid, nonanoic acid) and amino acid (oxoproline) and amine (diethanolamine) concentrations, which could be from the increased Proteobacteria abundance after CeO2 NP exposure.
32663007	0	132	theme	Relevant-Level	16:29	arg1	CeO2					31:34	Environmentally Relevant-Level CeO2 NP	0:37	Environmentally Relevant-Level CeO2 NP with Ferrous Amendment	0:60	Environmentally Relevant-Level CeO2 NP with Ferrous Amendment Alters Soil Bacterial Community Compositions and Metabolite Profiles in Rice-Planted Soils.
32663007	4	133	theme	bacterial	699:707	arg1	compositions					719:730	soil bacterial community compositions	694:730	soil bacterial community compositions	694:730	Results showed that CeO2 NP exposure changed soil bacterial community compositions and soil metabolomics, and the above changes were further shifted with the ferrous amendment.
32663007	2	134	theme	states	372:377	arg1	underground					379:389	the reduction states underground	358:389	the reduction states underground	358:389	The ferrous ion is rich in rhizosphere soil of rice due to the reduction states underground.
32663007	13	135	theme	fatty	2389:2393	arg1	acids					2395:2399	fatty acids	2389:2399	fatty acids	2389:2399	Activities of soil enzymes (urease, invertase, and cellulose), pH, and soil organic matter affected dominant metabolites containing fatty acids, inorganic acid, and sugar.
32663007	13	136	dep	enzymes	2276:2282	arg1	invertase					2293:2301	invertase	2293:2301	invertase	2293:2301	Activities of soil enzymes (urease, invertase, and cellulose), pH, and soil organic matter affected dominant metabolites containing fatty acids, inorganic acid, and sugar.
32663007	13	136	dep	enzymes	2276:2282	arg1	cellulose					2308:2316	cellulose	2308:2316	cellulose	2308:2316	Activities of soil enzymes (urease, invertase, and cellulose), pH, and soil organic matter affected dominant metabolites containing fatty acids, inorganic acid, and sugar.
32663007	13	136	dep	enzymes	2276:2282	arg1	urease					2285:2290	urease	2285:2290	urease	2285:2290	Activities of soil enzymes (urease, invertase, and cellulose), pH, and soil organic matter affected dominant metabolites containing fatty acids, inorganic acid, and sugar.
32663007	13	136	dep	enzymes	2276:2282	arg1	enzymes					2276:2282	soil enzymes	2271:2282	soil enzymes (urease, invertase, and cellulose)	2271:2317	Activities of soil enzymes (urease, invertase, and cellulose), pH, and soil organic matter affected dominant metabolites containing fatty acids, inorganic acid, and sugar.
32663007	3	137	theme	rice-planted	616:627	arg1	soil					629:632	rice-planted soil	616:632	rice-planted soil over 150 days	616:646	The aim of this study was to investigate the effects of environmentally relevant-level CeO2 NP (25 mg·kg-1) in the absence or presence of ferrous (30 mg·kg-1) amendment on soil bacterial communities and soil metabolomics in rice-planted soil over 150 days.
32663007	1	138	dep	risks	172:176	arg1	The					154:156	The	154:156	The	154:156	The environmental risks and benefits associated with the introduction of CeO2 nanoparticle (NP) in agricultural soil must be carefully assessed.
32663007	3	139	theme	relevant-level	464:477	arg1	NP					484:485	environmentally relevant-level CeO2 NP	448:485	environmentally relevant-level CeO2 NP (25 mg·kg-1) in the absence or presence of ferrous (30 mg·kg-1) amendment	448:559	The aim of this study was to investigate the effects of environmentally relevant-level CeO2 NP (25 mg·kg-1) in the absence or presence of ferrous (30 mg·kg-1) amendment on soil bacterial communities and soil metabolomics in rice-planted soil over 150 days.
32663007	3	139	theme	relevant-level	464:477	arg1	mg·kg-1					491:497	25 mg·kg-1	488:497	25 mg·kg-1	488:497	The aim of this study was to investigate the effects of environmentally relevant-level CeO2 NP (25 mg·kg-1) in the absence or presence of ferrous (30 mg·kg-1) amendment on soil bacterial communities and soil metabolomics in rice-planted soil over 150 days.
32663007	0	140	theme	Ferrous	44:50	arg1	Amendment					52:60	Ferrous Amendment	44:60	Ferrous Amendment	44:60	Environmentally Relevant-Level CeO2 NP with Ferrous Amendment Alters Soil Bacterial Community Compositions and Metabolite Profiles in Rice-Planted Soils.
32663007	11	141	theme	acid	1995:1998	arg1	metabolism					2000:2009	fatty acid metabolism	1989:2009	fatty acid metabolism	1989:2009	After adding CeO2 NP with ferrous or not, the main metabolic changes were concentrated on fluctuations in starch and sucrose metabolism, nitrogen metabolism, sulfur metabolism, propanoate metabolism, fatty acid metabolism, and urea cycle.
32663007	13	142	theme	organic	2333:2339	arg1	matter					2341:2346	soil organic matter	2328:2346	soil organic matter	2328:2346	Activities of soil enzymes (urease, invertase, and cellulose), pH, and soil organic matter affected dominant metabolites containing fatty acids, inorganic acid, and sugar.
32663007	3	143	theme	study	408:412	arg1	aim					396:398	The aim	392:398	The aim of this study	392:412	The aim of this study was to investigate the effects of environmentally relevant-level CeO2 NP (25 mg·kg-1) in the absence or presence of ferrous (30 mg·kg-1) amendment on soil bacterial communities and soil metabolomics in rice-planted soil over 150 days.
32663007	3	144	from	effects	437:443	arg1	presence					518:525	presence	518:525	presence	518:525	The aim of this study was to investigate the effects of environmentally relevant-level CeO2 NP (25 mg·kg-1) in the absence or presence of ferrous (30 mg·kg-1) amendment on soil bacterial communities and soil metabolomics in rice-planted soil over 150 days.
32663007	3	144	from	effects	437:443	arg1	communities					579:589	soil bacterial communities	564:589	soil bacterial communities	564:589	The aim of this study was to investigate the effects of environmentally relevant-level CeO2 NP (25 mg·kg-1) in the absence or presence of ferrous (30 mg·kg-1) amendment on soil bacterial communities and soil metabolomics in rice-planted soil over 150 days.
32663007	3	144	from	effects	437:443	arg1	absence					507:513	absence	507:513	absence	507:513	The aim of this study was to investigate the effects of environmentally relevant-level CeO2 NP (25 mg·kg-1) in the absence or presence of ferrous (30 mg·kg-1) amendment on soil bacterial communities and soil metabolomics in rice-planted soil over 150 days.
32663007	3	144	from	effects	437:443	arg1	metabolomics					600:611	soil metabolomics	595:611	soil metabolomics	595:611	The aim of this study was to investigate the effects of environmentally relevant-level CeO2 NP (25 mg·kg-1) in the absence or presence of ferrous (30 mg·kg-1) amendment on soil bacterial communities and soil metabolomics in rice-planted soil over 150 days.
32663007	11	145	theme	nitrogen	1926:1933	arg1	metabolism					1935:1944	nitrogen metabolism	1926:1944	nitrogen metabolism	1926:1944	After adding CeO2 NP with ferrous or not, the main metabolic changes were concentrated on fluctuations in starch and sucrose metabolism, nitrogen metabolism, sulfur metabolism, propanoate metabolism, fatty acid metabolism, and urea cycle.
32663007	14	146	theme	community	2490:2498	arg1	influence					2462:2470	the influence	2458:2470	the influence of soil bacterial community on metabolites	2458:2513	Network analysis showed that the influence of soil bacterial community on metabolites varied with metabolites and bacteria species.
32663007	5	147	theme	ferrous	1029:1035	arg1	amendment					1037:1045	ferrous amendment	1029:1045	ferrous amendment	1029:1045	Several functionally significant bacterial phyla containing Proteobacteria and Bacteroidetes abundances, which were associated with carbon and nitrogen cycling, were promoted after CeO2 NP exposure with ferrous amendment.
32663007	14	148	from	influence	2462:2470	arg1	metabolites					2503:2513	metabolites	2503:2513	metabolites	2503:2513	Network analysis showed that the influence of soil bacterial community on metabolites varied with metabolites and bacteria species.
32663007	15	149	dep	acids	2607:2611	arg1	acid					2623:2626	hexanoic acid	2614:2626	hexanoic acid	2614:2626	The presence of CeO2 NP mainly promoted fatty acids (hexanoic acid, nonanoic acid) and amino acid (oxoproline) and amine (diethanolamine) concentrations, which could be from the increased Proteobacteria abundance after CeO2 NP exposure.
32663007	15	149	dep	acids	2607:2611	arg1	acid					2638:2641	nonanoic acid	2629:2641	nonanoic acid	2629:2641	The presence of CeO2 NP mainly promoted fatty acids (hexanoic acid, nonanoic acid) and amino acid (oxoproline) and amine (diethanolamine) concentrations, which could be from the increased Proteobacteria abundance after CeO2 NP exposure.
32663007	13	150	theme	soil	2328:2331	arg1	matter					2341:2346	soil organic matter	2328:2346	soil organic matter	2328:2346	Activities of soil enzymes (urease, invertase, and cellulose), pH, and soil organic matter affected dominant metabolites containing fatty acids, inorganic acid, and sugar.
32663007	14	151	theme	soil	2475:2478	arg1	community					2490:2498	soil bacterial community	2475:2498	soil bacterial community	2475:2498	Network analysis showed that the influence of soil bacterial community on metabolites varied with metabolites and bacteria species.
32663007	13	152	theme	pH	2320:2321	arg1	Activities					2257:2266	Activities	2257:2266	Activities of soil enzymes (urease, invertase, and cellulose), pH, and soil organic matter	2257:2346	Activities of soil enzymes (urease, invertase, and cellulose), pH, and soil organic matter affected dominant metabolites containing fatty acids, inorganic acid, and sugar.
32663007	11	153	theme	propanoate	1966:1975	arg1	metabolism					1977:1986	propanoate metabolism	1966:1986	propanoate metabolism	1966:1986	After adding CeO2 NP with ferrous or not, the main metabolic changes were concentrated on fluctuations in starch and sucrose metabolism, nitrogen metabolism, sulfur metabolism, propanoate metabolism, fatty acid metabolism, and urea cycle.
32663007	7	154	theme	NP	1301:1302	arg1	exposure					1304:1311	CeO2 NP exposure	1296:1311	CeO2 NP exposure	1296:1311	Among rhizosphere soil enzyme activities, cellulose activity was the most sensitive for CeO2 NP exposure.
32663007	14	155	dep	metabolites	2527:2537	arg1	species					2552:2558	species	2552:2558	species	2552:2558	Network analysis showed that the influence of soil bacterial community on metabolites varied with metabolites and bacteria species.
32663007	8	156	theme	soil	1458:1461	arg1	activity					1473:1480	soil cellulose activity	1458:1480	soil cellulose activity	1458:1480	NP decreased Firmicutes and increased Chloroflexi, Rokubacteria, and Thaumarchaeota abundances at the phylum level, which contributed to reduce soil cellulose activity.
32663007	3	157	theme	NP	484:485	arg1	effects					437:443	the effects	433:443	the effects of environmentally relevant-level CeO2 NP (25 mg·kg-1) in the absence or presence of ferrous (30 mg·kg-1) amendment on soil bacterial communities and soil metabolomics	433:611	The aim of this study was to investigate the effects of environmentally relevant-level CeO2 NP (25 mg·kg-1) in the absence or presence of ferrous (30 mg·kg-1) amendment on soil bacterial communities and soil metabolomics in rice-planted soil over 150 days.
32663007	5	158	theme	CeO2	1007:1010	arg1	exposure					1015:1022	CeO2 NP exposure	1007:1022	CeO2 NP exposure with ferrous amendment	1007:1045	Several functionally significant bacterial phyla containing Proteobacteria and Bacteroidetes abundances, which were associated with carbon and nitrogen cycling, were promoted after CeO2 NP exposure with ferrous amendment.
32663007	17	159	theme	microbial	3035:3043	arg1	communities					3045:3055	soil microbial communities	3030:3055	soil microbial communities	3030:3055	These results provide valuable information for understanding the impact of environmentally relevant-level CeO2 NP exposure on soil microbial communities and metabolites with or without ferrous, which is needed to understand the ecological risk posed by long-term CeO2 NP exposure in rice-planted soil with rich ferrous.
32663007	1	160	theme	agricultural	253:264	arg1	soil					266:269	agricultural soil	253:269	agricultural soil	253:269	The environmental risks and benefits associated with the introduction of CeO2 nanoparticle (NP) in agricultural soil must be carefully assessed.
33539901	10	0	theme	glycans	2227:2233	arg1	abundance					2205:2213	the high abundance	2196:2213	the high abundance of O-linked glycans with sialylated antennae	2196:2258	These findings strongly suggest that hCTP enhances the in vivo biological activity of recombinant Japanese eel Gths due to the high abundance of O-linked glycans with sialylated antennae.
33539901	3	1	theme	complex	889:895	arg1	types					897:901	complex types	889:901	complex types	889:901	Lectin blot analyses demonstrated that all these recombinant Gths contained N-glycans of the high mannose and complex types.
33539901	5	2	theme	N-glycans	1139:1147	arg1	sialylation					1124:1134	sialylation	1124:1134	sialylation of N-glycans	1124:1147	Further analyses of glycans by liquid chromatography-mass spectrometry suggested that the species, amount, and degree of sialylation of N-glycans were comparable among recombinant Fshs and recombinant Lhs, while the amount of O-glycans with sialic acids in rGth-hCTPs was higher than that in the corresponding rGth-eCTPs.
33539901	4	3	theme	O-linked	976:983	arg1	oligosaccharides					985:1000	highly sialylated O-linked oligosaccharides	958:1000	highly sialylated O-linked oligosaccharides	958:1000	In contrast, only rGth-hCTPs and rGth-eCTPs possessed highly sialylated O-linked oligosaccharides.
33539901	0	4	theme	293-F	198:202	arg1	lines					209:213	FreeStyle 293-F cell lines	188:213	FreeStyle 293-F cell lines	188:213	C-terminal peptide (hCTP) of human chorionic gonadotropin enhances in vivo biological activity of recombinant Japanese eel follicle-stimulating hormone and luteinizing hormone produced in FreeStyle 293-F cell lines.
33539901	8	5	theme	11KT	1810:1813	arg1	levels					1800:1805	the serum levels	1790:1805	the serum levels of 11KT	1790:1813	The long-term treatment of male eels with these recombinant Gths also revealed the superiority of rGth-hCTPs in assisted reproduction; thus, the serum levels of 11KT and gonadosomatic indices in eels treated with rGth-hCTPs were higher than those in eels treated with the corresponding rGths and rGth-eCTPs.
33539901	8	5	theme	11KT	1810:1813	arg1	higher					1878:1883	higher	1878:1883	higher	1878:1883	The long-term treatment of male eels with these recombinant Gths also revealed the superiority of rGth-hCTPs in assisted reproduction; thus, the serum levels of 11KT and gonadosomatic indices in eels treated with rGth-hCTPs were higher than those in eels treated with the corresponding rGths and rGth-eCTPs.
33539901	8	5	theme	11KT	1810:1813	arg1	indices					1833:1839	gonadosomatic indices	1819:1839	gonadosomatic indices in eels treated with rGth-hCTPs	1819:1871	The long-term treatment of male eels with these recombinant Gths also revealed the superiority of rGth-hCTPs in assisted reproduction; thus, the serum levels of 11KT and gonadosomatic indices in eels treated with rGth-hCTPs were higher than those in eels treated with the corresponding rGths and rGth-eCTPs.
33539901	8	6	theme	long-term	1653:1661	arg1	treatment					1663:1671	The long-term treatment	1649:1671	The long-term treatment of male eels with these recombinant Gths	1649:1712	The long-term treatment of male eels with these recombinant Gths also revealed the superiority of rGth-hCTPs in assisted reproduction; thus, the serum levels of 11KT and gonadosomatic indices in eels treated with rGth-hCTPs were higher than those in eels treated with the corresponding rGths and rGth-eCTPs.
33539901	2	7	theme	extra	557:561	arg1	site					579:582	an extra O-glycosylation site	554:582	an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin)	554:668	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	4	8	contain	possessed	948:956	arg1	rGth-eCTPs					937:946	rGth-eCTPs	937:946	rGth-eCTPs	937:946	In contrast, only rGth-hCTPs and rGth-eCTPs possessed highly sialylated O-linked oligosaccharides.
33539901	4	8	contain	possessed	948:956	arg2	oligosaccharides					985:1000	highly sialylated O-linked oligosaccharides	958:1000	highly sialylated O-linked oligosaccharides	958:1000	In contrast, only rGth-hCTPs and rGth-eCTPs possessed highly sialylated O-linked oligosaccharides.
33539901	4	8	contain	possessed	948:956	arg1	rGth-hCTPs					922:931	rGth-hCTPs	922:931	rGth-hCTPs	922:931	In contrast, only rGth-hCTPs and rGth-eCTPs possessed highly sialylated O-linked oligosaccharides.
33539901	2	9	theme	gonadotropin	656:667	arg1	peptide					605:611	a C-terminal peptide	592:611	a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin	592:667	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	8	10	theme	gonadosomatic	1819:1831	arg1	indices					1833:1839	gonadosomatic indices	1819:1839	gonadosomatic indices in eels treated with rGth-hCTPs	1819:1871	The long-term treatment of male eels with these recombinant Gths also revealed the superiority of rGth-hCTPs in assisted reproduction; thus, the serum levels of 11KT and gonadosomatic indices in eels treated with rGth-hCTPs were higher than those in eels treated with the corresponding rGths and rGth-eCTPs.
33539901	2	11	dep	site	579:582	arg1	peptide					605:611	a C-terminal peptide	592:611	a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin	592:667	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	8	12	from	superiority	1732:1742	arg1	reproduction					1770:1781	assisted reproduction	1761:1781	assisted reproduction	1761:1781	The long-term treatment of male eels with these recombinant Gths also revealed the superiority of rGth-hCTPs in assisted reproduction; thus, the serum levels of 11KT and gonadosomatic indices in eels treated with rGth-hCTPs were higher than those in eels treated with the corresponding rGths and rGth-eCTPs.
33539901	0	13	theme	Japanese	110:117	arg1	hormone					144:150	recombinant Japanese eel follicle-stimulating hormone	98:150	recombinant Japanese eel follicle-stimulating hormone	98:150	C-terminal peptide (hCTP) of human chorionic gonadotropin enhances in vivo biological activity of recombinant Japanese eel follicle-stimulating hormone and luteinizing hormone produced in FreeStyle 293-F cell lines.
33539901	8	14	theme	eels	1681:1684	arg1	treatment					1663:1671	The long-term treatment	1649:1671	The long-term treatment of male eels with these recombinant Gths	1649:1712	The long-term treatment of male eels with these recombinant Gths also revealed the superiority of rGth-hCTPs in assisted reproduction; thus, the serum levels of 11KT and gonadosomatic indices in eels treated with rGth-hCTPs were higher than those in eels treated with the corresponding rGths and rGth-eCTPs.
33539901	5	15	from	acids	1251:1255	arg1	rGth-hCTPs					1260:1269	rGth-hCTPs	1260:1269	rGth-hCTPs	1260:1269	Further analyses of glycans by liquid chromatography-mass spectrometry suggested that the species, amount, and degree of sialylation of N-glycans were comparable among recombinant Fshs and recombinant Lhs, while the amount of O-glycans with sialic acids in rGth-hCTPs was higher than that in the corresponding rGth-eCTPs.
33539901	0	16	theme	follicle-stimulating	123:142	arg1	hormone					144:150	recombinant Japanese eel follicle-stimulating hormone	98:150	recombinant Japanese eel follicle-stimulating hormone	98:150	C-terminal peptide (hCTP) of human chorionic gonadotropin enhances in vivo biological activity of recombinant Japanese eel follicle-stimulating hormone and luteinizing hormone produced in FreeStyle 293-F cell lines.
33539901	2	17	with	Gths	419:422	arg1	site					579:582	an extra O-glycosylation site	554:582	an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin)	554:668	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	4	18	gly	sialylated	965:974	arg1	oligosaccharides					985:1000	highly sialylated O-linked oligosaccharides	958:1000	highly sialylated O-linked oligosaccharides	958:1000	In contrast, only rGth-hCTPs and rGth-eCTPs possessed highly sialylated O-linked oligosaccharides.
33539901	0	19	theme	hormone	168:174	arg1	activity					86:93	in vivo biological activity	67:93	in vivo biological activity of recombinant Japanese eel follicle-stimulating hormone and luteinizing hormone produced in FreeStyle 293-F cell lines	67:213	C-terminal peptide (hCTP) of human chorionic gonadotropin enhances in vivo biological activity of recombinant Japanese eel follicle-stimulating hormone and luteinizing hormone produced in FreeStyle 293-F cell lines.
33539901	2	20	theme	recombinant	450:460	arg1	Gths					471:474	recombinant chimeric Gths	450:474	recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified	450:776	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	5	21	theme	O-glycans	1229:1237	arg1	amount					1219:1224	the amount	1215:1224	the amount of O-glycans with sialic acids in rGth-hCTPs	1215:1269	Further analyses of glycans by liquid chromatography-mass spectrometry suggested that the species, amount, and degree of sialylation of N-glycans were comparable among recombinant Fshs and recombinant Lhs, while the amount of O-glycans with sialic acids in rGth-hCTPs was higher than that in the corresponding rGth-eCTPs.
33539901	5	21	theme	O-glycans	1229:1237	arg1	higher					1275:1280	higher	1275:1280	higher	1275:1280	Further analyses of glycans by liquid chromatography-mass spectrometry suggested that the species, amount, and degree of sialylation of N-glycans were comparable among recombinant Fshs and recombinant Lhs, while the amount of O-glycans with sialic acids in rGth-hCTPs was higher than that in the corresponding rGth-eCTPs.
33539901	5	21	theme	O-glycans	1229:1237	arg1	O-glycans					1229:1237	O-glycans	1229:1237	O-glycans with sialic acids in rGth-hCTPs	1229:1269	Further analyses of glycans by liquid chromatography-mass spectrometry suggested that the species, amount, and degree of sialylation of N-glycans were comparable among recombinant Fshs and recombinant Lhs, while the amount of O-glycans with sialic acids in rGth-hCTPs was higher than that in the corresponding rGth-eCTPs.
33539901	0	22	theme	gonadotropin	45:56	arg1	hCTP					20:23	hCTP	20:23	hCTP	20:23	C-terminal peptide (hCTP) of human chorionic gonadotropin enhances in vivo biological activity of recombinant Japanese eel follicle-stimulating hormone and luteinizing hormone produced in FreeStyle 293-F cell lines.
33539901	0	22	theme	gonadotropin	45:56	arg1	peptide					11:17	C-terminal peptide	0:17	C-terminal peptide (hCTP) of human chorionic gonadotropin	0:56	C-terminal peptide (hCTP) of human chorionic gonadotropin enhances in vivo biological activity of recombinant Japanese eel follicle-stimulating hormone and luteinizing hormone produced in FreeStyle 293-F cell lines.
33539901	1	23	theme	central	308:314	arg1	roles					316:320	central roles	308:320	central roles	308:320	Gonadotropins (Gths), follicle-stimulating hormone (Fsh), and luteinizing hormone (Lh) play central roles in the reproductive biology of vertebrates.
33539901	3	24	theme	blot	786:789	arg1	analyses					791:798	Lectin blot analyses	779:798	Lectin blot analyses	779:798	Lectin blot analyses demonstrated that all these recombinant Gths contained N-glycans of the high mannose and complex types.
33539901	0	25	theme	in	67:68	arg1	activity					86:93	in vivo biological activity	67:93	in vivo biological activity of recombinant Japanese eel follicle-stimulating hormone and luteinizing hormone produced in FreeStyle 293-F cell lines	67:213	C-terminal peptide (hCTP) of human chorionic gonadotropin enhances in vivo biological activity of recombinant Japanese eel follicle-stimulating hormone and luteinizing hormone produced in FreeStyle 293-F cell lines.
33539901	8	26	theme	rGth-hCTPs	1747:1756	arg1	superiority					1732:1742	the superiority	1728:1742	the superiority of rGth-hCTPs in assisted reproduction	1728:1781	The long-term treatment of male eels with these recombinant Gths also revealed the superiority of rGth-hCTPs in assisted reproduction; thus, the serum levels of 11KT and gonadosomatic indices in eels treated with rGth-hCTPs were higher than those in eels treated with the corresponding rGths and rGth-eCTPs.
33539901	10	27	theme	eel	2180:2182	arg1	Gths					2184:2187	recombinant Japanese eel Gths	2159:2187	recombinant Japanese eel Gths	2159:2187	These findings strongly suggest that hCTP enhances the in vivo biological activity of recombinant Japanese eel Gths due to the high abundance of O-linked glycans with sialylated antennae.
33539901	9	28	located	observed	2036:2043	arg1	eels					2067:2070	rGth-hCTPs-treated eels	2048:2070	rGth-hCTPs-treated eels	2048:2070	The induction of the entire process of spermatogenesis was only histologically observed in rGth-hCTPs-treated eels.
33539901	9	28	located	observed	2036:2043	arg2	induction					1961:1969	The induction	1957:1969	The induction of the entire process of spermatogenesis	1957:2010	The induction of the entire process of spermatogenesis was only histologically observed in rGth-hCTPs-treated eels.
33539901	0	29	theme	biological	75:84	arg1	activity					86:93	in vivo biological activity	67:93	in vivo biological activity of recombinant Japanese eel follicle-stimulating hormone and luteinizing hormone produced in FreeStyle 293-F cell lines	67:213	C-terminal peptide (hCTP) of human chorionic gonadotropin enhances in vivo biological activity of recombinant Japanese eel follicle-stimulating hormone and luteinizing hormone produced in FreeStyle 293-F cell lines.
33539901	5	30	theme	glycans	1023:1029	arg1	analyses					1011:1018	Further analyses	1003:1018	Further analyses of glycans by liquid chromatography-mass spectrometry	1003:1072	Further analyses of glycans by liquid chromatography-mass spectrometry suggested that the species, amount, and degree of sialylation of N-glycans were comparable among recombinant Fshs and recombinant Lhs, while the amount of O-glycans with sialic acids in rGth-hCTPs was higher than that in the corresponding rGth-eCTPs.
33539901	8	31	theme	assisted	1761:1768	arg1	reproduction					1770:1781	assisted reproduction	1761:1781	assisted reproduction	1761:1781	The long-term treatment of male eels with these recombinant Gths also revealed the superiority of rGth-hCTPs in assisted reproduction; thus, the serum levels of 11KT and gonadosomatic indices in eels treated with rGth-hCTPs were higher than those in eels treated with the corresponding rGths and rGth-eCTPs.
33539901	10	32	dep	in	2128:2129	arg1	vivo					2131:2134	vivo	2131:2134	vivo	2131:2134	These findings strongly suggest that hCTP enhances the in vivo biological activity of recombinant Japanese eel Gths due to the high abundance of O-linked glycans with sialylated antennae.
33539901	10	33	theme	recombinant	2159:2169	arg1	Gths					2184:2187	recombinant Japanese eel Gths	2159:2187	recombinant Japanese eel Gths	2159:2187	These findings strongly suggest that hCTP enhances the in vivo biological activity of recombinant Japanese eel Gths due to the high abundance of O-linked glycans with sialylated antennae.
33539901	8	34	theme	corresponding	1921:1933	arg1	rGths					1935:1939	the corresponding rGths	1917:1939	the corresponding rGths	1917:1939	The long-term treatment of male eels with these recombinant Gths also revealed the superiority of rGth-hCTPs in assisted reproduction; thus, the serum levels of 11KT and gonadosomatic indices in eels treated with rGth-hCTPs were higher than those in eels treated with the corresponding rGths and rGth-eCTPs.
33539901	5	35	theme	liquid	1034:1039	arg1	spectrometry					1061:1072	liquid chromatography-mass spectrometry	1034:1072	liquid chromatography-mass spectrometry	1034:1072	Further analyses of glycans by liquid chromatography-mass spectrometry suggested that the species, amount, and degree of sialylation of N-glycans were comparable among recombinant Fshs and recombinant Lhs, while the amount of O-glycans with sialic acids in rGth-hCTPs was higher than that in the corresponding rGth-eCTPs.
33539901	2	36	with	Gths	471:474	arg1	site					579:582	an extra O-glycosylation site	554:582	an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin)	554:668	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	2	37	theme	human	616:620	arg1	peptide					605:611	a C-terminal peptide	592:611	a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin	592:667	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	0	38	theme	C-terminal	0:9	arg1	hCTP					20:23	hCTP	20:23	hCTP	20:23	C-terminal peptide (hCTP) of human chorionic gonadotropin enhances in vivo biological activity of recombinant Japanese eel follicle-stimulating hormone and luteinizing hormone produced in FreeStyle 293-F cell lines.
33539901	0	38	theme	C-terminal	0:9	arg1	peptide					11:17	C-terminal peptide	0:17	C-terminal peptide (hCTP) of human chorionic gonadotropin	0:56	C-terminal peptide (hCTP) of human chorionic gonadotropin enhances in vivo biological activity of recombinant Japanese eel follicle-stimulating hormone and luteinizing hormone produced in FreeStyle 293-F cell lines.
33539901	0	39	dep	in	67:68	arg1	vivo					70:73	vivo	70:73	vivo	70:73	C-terminal peptide (hCTP) of human chorionic gonadotropin enhances in vivo biological activity of recombinant Japanese eel follicle-stimulating hormone and luteinizing hormone produced in FreeStyle 293-F cell lines.
33539901	7	40	theme	serum	1599:1603	arg1	fluctuations					1605:1616	the serum fluctuations	1595:1616	the serum fluctuations of 11-ketotestosterone (11KT)	1595:1646	The levels of rGth-hCTPs tended to be higher than those of the corresponding rGths and rGth-eCTPs throughout the experimental period, coinciding with the serum fluctuations of 11-ketotestosterone (11KT).
33539901	8	41	from	levels	1800:1805	arg1	eels					1844:1847	eels	1844:1847	eels treated with rGth-hCTPs	1844:1871	The long-term treatment of male eels with these recombinant Gths also revealed the superiority of rGth-hCTPs in assisted reproduction; thus, the serum levels of 11KT and gonadosomatic indices in eels treated with rGth-hCTPs were higher than those in eels treated with the corresponding rGths and rGth-eCTPs.
33539901	9	42	theme	entire	1978:1983	arg1	process					1985:1991	the entire process	1974:1991	the entire process of spermatogenesis	1974:2010	The induction of the entire process of spermatogenesis was only histologically observed in rGth-hCTPs-treated eels.
33539901	5	43	theme	sialic	1244:1249	arg1	acids					1251:1255	sialic acids	1244:1255	sialic acids in rGth-hCTPs	1244:1269	Further analyses of glycans by liquid chromatography-mass spectrometry suggested that the species, amount, and degree of sialylation of N-glycans were comparable among recombinant Fshs and recombinant Lhs, while the amount of O-glycans with sialic acids in rGth-hCTPs was higher than that in the corresponding rGth-eCTPs.
33539901	2	44	theme	Japanese	406:413	arg1	Gths					419:422	recombinant single-chain Japanese eel Gths	381:422	recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh)	381:443	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	1	45	theme	luteinizing	278:288	arg1	Lh					299:300	Lh	299:300	Lh	299:300	Gonadotropins (Gths), follicle-stimulating hormone (Fsh), and luteinizing hormone (Lh) play central roles in the reproductive biology of vertebrates.
33539901	1	45	theme	luteinizing	278:288	arg1	hormone					290:296	luteinizing hormone	278:296	luteinizing hormone (Lh)	278:301	Gonadotropins (Gths), follicle-stimulating hormone (Fsh), and luteinizing hormone (Lh) play central roles in the reproductive biology of vertebrates.
33539901	3	46	theme	recombinant	828:838	arg1	Gths					840:843	all these recombinant Gths	818:843	all these recombinant Gths	818:843	Lectin blot analyses demonstrated that all these recombinant Gths contained N-glycans of the high mannose and complex types.
33539901	10	47	gly	sialylated	2240:2249	arg1	antennae					2251:2258	sialylated antennae	2240:2258	sialylated antennae	2240:2258	These findings strongly suggest that hCTP enhances the in vivo biological activity of recombinant Japanese eel Gths due to the high abundance of O-linked glycans with sialylated antennae.
33539901	2	48	theme	recombinant	381:391	arg1	Gths					419:422	recombinant single-chain Japanese eel Gths	381:422	recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh)	381:443	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	6	49	theme	recombinant	1345:1355	arg1	Gths					1357:1360	recombinant Gths	1345:1360	recombinant Gths	1345:1360	The serum levels of recombinant Gths in male eels significantly increased 12-24 h after a single injection of the Gths.
33539901	3	50	theme	high	872:875	arg1	mannose					877:883	the high mannose	868:883	the high mannose	868:883	Lectin blot analyses demonstrated that all these recombinant Gths contained N-glycans of the high mannose and complex types.
33539901	0	51	theme	human	29:33	arg1	gonadotropin					45:56	human chorionic gonadotropin	29:56	human chorionic gonadotropin	29:56	C-terminal peptide (hCTP) of human chorionic gonadotropin enhances in vivo biological activity of recombinant Japanese eel follicle-stimulating hormone and luteinizing hormone produced in FreeStyle 293-F cell lines.
33539901	2	52	theme	glycoprotein	715:726	arg1	half-life					702:710	the half-life	698:710	the half-life of glycoprotein	698:726	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	3	53	theme	types	897:901	arg1	N-glycans					855:863	N-glycans	855:863	N-glycans of the high mannose and complex types	855:901	Lectin blot analyses demonstrated that all these recombinant Gths contained N-glycans of the high mannose and complex types.
33539901	10	54	with	glycans	2227:2233	arg1	antennae					2251:2258	sialylated antennae	2240:2258	sialylated antennae	2240:2258	These findings strongly suggest that hCTP enhances the in vivo biological activity of recombinant Japanese eel Gths due to the high abundance of O-linked glycans with sialylated antennae.
33539901	6	55	theme	single	1415:1420	arg1	injection					1422:1430	a single injection	1413:1430	a single injection of the Gths	1413:1442	The serum levels of recombinant Gths in male eels significantly increased 12-24 h after a single injection of the Gths.
33539901	8	56	theme	serum	1794:1798	arg1	levels					1800:1805	the serum levels	1790:1805	the serum levels of 11KT	1790:1813	The long-term treatment of male eels with these recombinant Gths also revealed the superiority of rGth-hCTPs in assisted reproduction; thus, the serum levels of 11KT and gonadosomatic indices in eels treated with rGth-hCTPs were higher than those in eels treated with the corresponding rGths and rGth-eCTPs.
33539901	8	56	theme	serum	1794:1798	arg1	higher					1878:1883	higher	1878:1883	higher	1878:1883	The long-term treatment of male eels with these recombinant Gths also revealed the superiority of rGth-hCTPs in assisted reproduction; thus, the serum levels of 11KT and gonadosomatic indices in eels treated with rGth-hCTPs were higher than those in eels treated with the corresponding rGths and rGth-eCTPs.
33539901	4	57	theme	sialylated	965:974	arg1	oligosaccharides					985:1000	highly sialylated O-linked oligosaccharides	958:1000	highly sialylated O-linked oligosaccharides	958:1000	In contrast, only rGth-hCTPs and rGth-eCTPs possessed highly sialylated O-linked oligosaccharides.
33539901	0	58	theme	FreeStyle	188:196	arg1	lines					209:213	FreeStyle 293-F cell lines	188:213	FreeStyle 293-F cell lines	188:213	C-terminal peptide (hCTP) of human chorionic gonadotropin enhances in vivo biological activity of recombinant Japanese eel follicle-stimulating hormone and luteinizing hormone produced in FreeStyle 293-F cell lines.
33539901	5	59	theme	corresponding	1299:1311	arg1	rGth-eCTPs					1313:1322	the corresponding rGth-eCTPs	1295:1322	the corresponding rGth-eCTPs	1295:1322	Further analyses of glycans by liquid chromatography-mass spectrometry suggested that the species, amount, and degree of sialylation of N-glycans were comparable among recombinant Fshs and recombinant Lhs, while the amount of O-glycans with sialic acids in rGth-hCTPs was higher than that in the corresponding rGth-eCTPs.
33539901	10	60	theme	O-linked	2218:2225	arg1	glycans					2227:2233	O-linked glycans	2218:2233	O-linked glycans with sialylated antennae	2218:2258	These findings strongly suggest that hCTP enhances the in vivo biological activity of recombinant Japanese eel Gths due to the high abundance of O-linked glycans with sialylated antennae.
33539901	0	61	theme	cell	204:207	arg1	lines					209:213	FreeStyle 293-F cell lines	188:213	FreeStyle 293-F cell lines	188:213	C-terminal peptide (hCTP) of human chorionic gonadotropin enhances in vivo biological activity of recombinant Japanese eel follicle-stimulating hormone and luteinizing hormone produced in FreeStyle 293-F cell lines.
33539901	2	62	gly	O-glycosylation	563:577	arg2	site					579:582	an extra O-glycosylation site	554:582	an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin)	554:668	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	2	63	theme	O-glycosylation	563:577	arg1	site					579:582	an extra O-glycosylation site	554:582	an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin)	554:668	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	4	64	link	O-linked	976:983	arg1	oligosaccharides					985:1000	highly sialylated O-linked oligosaccharides	958:1000	highly sialylated O-linked oligosaccharides	958:1000	In contrast, only rGth-hCTPs and rGth-eCTPs possessed highly sialylated O-linked oligosaccharides.
33539901	0	65	theme	eel	119:121	arg1	hormone					144:150	recombinant Japanese eel follicle-stimulating hormone	98:150	recombinant Japanese eel follicle-stimulating hormone	98:150	C-terminal peptide (hCTP) of human chorionic gonadotropin enhances in vivo biological activity of recombinant Japanese eel follicle-stimulating hormone and luteinizing hormone produced in FreeStyle 293-F cell lines.
33539901	10	66	theme	Gths	2184:2187	arg1	activity					2147:2154	the in vivo biological activity	2124:2154	the in vivo biological activity of recombinant Japanese eel Gths	2124:2187	These findings strongly suggest that hCTP enhances the in vivo biological activity of recombinant Japanese eel Gths due to the high abundance of O-linked glycans with sialylated antennae.
33539901	7	67	theme	experimental	1558:1569	arg1	period					1571:1576	the experimental period	1554:1576	the experimental period	1554:1576	The levels of rGth-hCTPs tended to be higher than those of the corresponding rGths and rGth-eCTPs throughout the experimental period, coinciding with the serum fluctuations of 11-ketotestosterone (11KT).
33539901	0	68	theme	hormone	144:150	arg1	activity					86:93	in vivo biological activity	67:93	in vivo biological activity of recombinant Japanese eel follicle-stimulating hormone and luteinizing hormone produced in FreeStyle 293-F cell lines	67:213	C-terminal peptide (hCTP) of human chorionic gonadotropin enhances in vivo biological activity of recombinant Japanese eel follicle-stimulating hormone and luteinizing hormone produced in FreeStyle 293-F cell lines.
33539901	5	69	theme	Further	1003:1009	arg1	analyses					1011:1018	Further analyses	1003:1018	Further analyses of glycans by liquid chromatography-mass spectrometry	1003:1072	Further analyses of glycans by liquid chromatography-mass spectrometry suggested that the species, amount, and degree of sialylation of N-glycans were comparable among recombinant Fshs and recombinant Lhs, while the amount of O-glycans with sialic acids in rGth-hCTPs was higher than that in the corresponding rGth-eCTPs.
33539901	6	70	theme	Gths	1439:1442	arg1	injection					1422:1430	a single injection	1413:1430	a single injection of the Gths	1413:1442	The serum levels of recombinant Gths in male eels significantly increased 12-24 h after a single injection of the Gths.
33539901	3	71	contain	contained	845:853	arg2	N-glycans					855:863	N-glycans	855:863	N-glycans of the high mannose and complex types	855:901	Lectin blot analyses demonstrated that all these recombinant Gths contained N-glycans of the high mannose and complex types.
33539901	3	71	contain	contained	845:853	arg1	Gths					840:843	all these recombinant Gths	818:843	all these recombinant Gths	818:843	Lectin blot analyses demonstrated that all these recombinant Gths contained N-glycans of the high mannose and complex types.
33539901	2	72	theme	equine	632:637	arg1	eCTP					640:643	eCTP	640:643	eCTP	640:643	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	2	72	theme	equine	632:637	arg1	gonadotropin					656:667	human (hCTP) or equine (eCTP) chorionic gonadotropin	616:667	gonadotropin	656:667	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	0	73	theme	luteinizing	156:166	arg1	hormone					168:174	luteinizing hormone	156:174	luteinizing hormone	156:174	C-terminal peptide (hCTP) of human chorionic gonadotropin enhances in vivo biological activity of recombinant Japanese eel follicle-stimulating hormone and luteinizing hormone produced in FreeStyle 293-F cell lines.
33539901	5	74	gly	sialylation	1124:1134	arg1	N-glycans					1139:1147	N-glycans	1139:1147	N-glycans	1139:1147	Further analyses of glycans by liquid chromatography-mass spectrometry suggested that the species, amount, and degree of sialylation of N-glycans were comparable among recombinant Fshs and recombinant Lhs, while the amount of O-glycans with sialic acids in rGth-hCTPs was higher than that in the corresponding rGth-eCTPs.
33539901	6	75	from	levels	1335:1340	arg1	eels					1370:1373	male eels	1365:1373	male eels	1365:1373	The serum levels of recombinant Gths in male eels significantly increased 12-24 h after a single injection of the Gths.
33539901	8	76	theme	recombinant	1697:1707	arg1	Gths					1709:1712	these recombinant Gths	1691:1712	these recombinant Gths	1691:1712	The long-term treatment of male eels with these recombinant Gths also revealed the superiority of rGth-hCTPs in assisted reproduction; thus, the serum levels of 11KT and gonadosomatic indices in eels treated with rGth-hCTPs were higher than those in eels treated with the corresponding rGths and rGth-eCTPs.
33539901	2	77	dep	peptide	605:611	arg1	either					585:590	either	585:590	either	585:590	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	7	78	theme	corresponding	1508:1520	arg1	rGths					1522:1526	the corresponding rGths	1504:1526	the corresponding rGths	1504:1526	The levels of rGth-hCTPs tended to be higher than those of the corresponding rGths and rGth-eCTPs throughout the experimental period, coinciding with the serum fluctuations of 11-ketotestosterone (11KT).
33539901	2	79	theme	chorionic	646:654	arg1	eCTP					640:643	eCTP	640:643	eCTP	640:643	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	2	79	theme	chorionic	646:654	arg1	gonadotropin					656:667	human (hCTP) or equine (eCTP) chorionic gonadotropin	616:667	gonadotropin	656:667	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	6	80	theme	serum	1329:1333	arg1	levels					1335:1340	The serum levels	1325:1340	The serum levels of recombinant Gths in male eels	1325:1373	The serum levels of recombinant Gths in male eels significantly increased 12-24 h after a single injection of the Gths.
33539901	10	81	theme	biological	2136:2145	arg1	activity					2147:2154	the in vivo biological activity	2124:2154	the in vivo biological activity of recombinant Japanese eel Gths	2124:2187	These findings strongly suggest that hCTP enhances the in vivo biological activity of recombinant Japanese eel Gths due to the high abundance of O-linked glycans with sialylated antennae.
33539901	5	82	theme	recombinant	1192:1202	arg1	Lhs					1204:1206	recombinant Lhs	1192:1206	recombinant Lhs	1192:1206	Further analyses of glycans by liquid chromatography-mass spectrometry suggested that the species, amount, and degree of sialylation of N-glycans were comparable among recombinant Fshs and recombinant Lhs, while the amount of O-glycans with sialic acids in rGth-hCTPs was higher than that in the corresponding rGth-eCTPs.
33539901	1	83	theme	reproductive	329:340	arg1	biology					342:348	the reproductive biology	325:348	the reproductive biology of vertebrates	325:363	Gonadotropins (Gths), follicle-stimulating hormone (Fsh), and luteinizing hormone (Lh) play central roles in the reproductive biology of vertebrates.
33539901	2	84	theme	C-terminal	594:603	arg1	peptide					605:611	a C-terminal peptide	592:611	a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin	592:667	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	3	85	theme	Lectin	779:784	arg1	analyses					791:798	Lectin blot analyses	779:798	Lectin blot analyses	779:798	Lectin blot analyses demonstrated that all these recombinant Gths contained N-glycans of the high mannose and complex types.
33539901	10	86	theme	Japanese	2171:2178	arg1	Gths					2184:2187	recombinant Japanese eel Gths	2159:2187	recombinant Japanese eel Gths	2159:2187	These findings strongly suggest that hCTP enhances the in vivo biological activity of recombinant Japanese eel Gths due to the high abundance of O-linked glycans with sialylated antennae.
33539901	0	87	theme	recombinant	98:108	arg1	hormone					144:150	recombinant Japanese eel follicle-stimulating hormone	98:150	recombinant Japanese eel follicle-stimulating hormone	98:150	C-terminal peptide (hCTP) of human chorionic gonadotropin enhances in vivo biological activity of recombinant Japanese eel follicle-stimulating hormone and luteinizing hormone produced in FreeStyle 293-F cell lines.
33539901	5	88	with	O-glycans	1229:1237	arg1	acids					1251:1255	sialic acids	1244:1255	sialic acids in rGth-hCTPs	1244:1269	Further analyses of glycans by liquid chromatography-mass spectrometry suggested that the species, amount, and degree of sialylation of N-glycans were comparable among recombinant Fshs and recombinant Lhs, while the amount of O-glycans with sialic acids in rGth-hCTPs was higher than that in the corresponding rGth-eCTPs.
33539901	2	89	dep	Gths	419:422	arg1	rGth					425:428	rGth	425:428	rGth	425:428	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	2	89	dep	Gths	419:422	arg1	study					374:378	this study	369:378	this study	369:378	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	2	90	theme	chimeric	462:469	arg1	Gths					471:474	recombinant chimeric Gths	450:474	recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified	450:776	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	10	91	theme	high	2200:2203	arg1	abundance					2205:2213	the high abundance	2196:2213	the high abundance of O-linked glycans with sialylated antennae	2196:2258	These findings strongly suggest that hCTP enhances the in vivo biological activity of recombinant Japanese eel Gths due to the high abundance of O-linked glycans with sialylated antennae.
33539901	5	92	theme	chromatography-mass	1041:1059	arg1	spectrometry					1061:1072	liquid chromatography-mass spectrometry	1034:1072	liquid chromatography-mass spectrometry	1034:1072	Further analyses of glycans by liquid chromatography-mass spectrometry suggested that the species, amount, and degree of sialylation of N-glycans were comparable among recombinant Fshs and recombinant Lhs, while the amount of O-glycans with sialic acids in rGth-hCTPs was higher than that in the corresponding rGth-eCTPs.
33539901	2	93	dep	rGth	425:428	arg1	rFsh					431:434	rFsh	431:434	rFsh	431:434	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	2	93	dep	rGth	425:428	arg1	rLh					440:442	rLh	440:442	rLh	440:442	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	7	94	theme	11-ketotestosterone	1621:1639	arg1	fluctuations					1605:1616	the serum fluctuations	1595:1616	the serum fluctuations of 11-ketotestosterone (11KT)	1595:1646	The levels of rGth-hCTPs tended to be higher than those of the corresponding rGths and rGth-eCTPs throughout the experimental period, coinciding with the serum fluctuations of 11-ketotestosterone (11KT).
33539901	8	95	with	treatment	1663:1671	arg1	Gths					1709:1712	these recombinant Gths	1691:1712	these recombinant Gths	1691:1712	The long-term treatment of male eels with these recombinant Gths also revealed the superiority of rGth-hCTPs in assisted reproduction; thus, the serum levels of 11KT and gonadosomatic indices in eels treated with rGth-hCTPs were higher than those in eels treated with the corresponding rGths and rGth-eCTPs.
33539901	2	96	gly	glycoprotein	715:726	arg1	glycoprotein					715:726	glycoprotein	715:726	glycoprotein	715:726	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	6	97	theme	male	1365:1368	arg1	eels					1370:1373	male eels	1365:1373	male eels	1365:1373	The serum levels of recombinant Gths in male eels significantly increased 12-24 h after a single injection of the Gths.
33539901	2	98	dep	Gths	471:474	arg1	rGth-hCTPs					477:486	rGth-hCTPs	477:486	rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP	477:546	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	2	99	theme	eel	415:417	arg1	Gths					419:422	recombinant single-chain Japanese eel Gths	381:422	recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh)	381:443	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	9	100	theme	process	1985:1991	arg1	induction					1961:1969	The induction	1957:1969	The induction of the entire process of spermatogenesis	1957:2010	The induction of the entire process of spermatogenesis was only histologically observed in rGth-hCTPs-treated eels.
33539901	8	101	theme	male	1676:1679	arg1	eels					1681:1684	male eels	1676:1684	male eels	1676:1684	The long-term treatment of male eels with these recombinant Gths also revealed the superiority of rGth-hCTPs in assisted reproduction; thus, the serum levels of 11KT and gonadosomatic indices in eels treated with rGth-hCTPs were higher than those in eels treated with the corresponding rGths and rGth-eCTPs.
33539901	2	102	theme	single-chain	393:404	arg1	Gths					419:422	recombinant single-chain Japanese eel Gths	381:422	recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh)	381:443	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	6	103	theme	Gths	1357:1360	arg1	levels					1335:1340	The serum levels	1325:1340	The serum levels of recombinant Gths in male eels	1325:1373	The serum levels of recombinant Gths in male eels significantly increased 12-24 h after a single injection of the Gths.
33539901	5	104	theme	sialylation	1124:1134	arg1	amount					1102:1107	amount	1102:1107	amount	1102:1107	Further analyses of glycans by liquid chromatography-mass spectrometry suggested that the species, amount, and degree of sialylation of N-glycans were comparable among recombinant Fshs and recombinant Lhs, while the amount of O-glycans with sialic acids in rGth-hCTPs was higher than that in the corresponding rGth-eCTPs.
33539901	5	104	theme	sialylation	1124:1134	arg1	degree					1114:1119	degree	1114:1119	degree of sialylation of N-glycans	1114:1147	Further analyses of glycans by liquid chromatography-mass spectrometry suggested that the species, amount, and degree of sialylation of N-glycans were comparable among recombinant Fshs and recombinant Lhs, while the amount of O-glycans with sialic acids in rGth-hCTPs was higher than that in the corresponding rGth-eCTPs.
33539901	5	104	theme	sialylation	1124:1134	arg1	species					1093:1099	the species	1089:1099	the species	1089:1099	Further analyses of glycans by liquid chromatography-mass spectrometry suggested that the species, amount, and degree of sialylation of N-glycans were comparable among recombinant Fshs and recombinant Lhs, while the amount of O-glycans with sialic acids in rGth-hCTPs was higher than that in the corresponding rGth-eCTPs.
33539901	5	104	theme	sialylation	1124:1134	arg1	comparable					1154:1163	comparable	1154:1163	comparable	1154:1163	Further analyses of glycans by liquid chromatography-mass spectrometry suggested that the species, amount, and degree of sialylation of N-glycans were comparable among recombinant Fshs and recombinant Lhs, while the amount of O-glycans with sialic acids in rGth-hCTPs was higher than that in the corresponding rGth-eCTPs.
33539901	5	104	theme	sialylation	1124:1134	arg1	sialylation					1124:1134	sialylation	1124:1134	sialylation of N-glycans	1124:1147	Further analyses of glycans by liquid chromatography-mass spectrometry suggested that the species, amount, and degree of sialylation of N-glycans were comparable among recombinant Fshs and recombinant Lhs, while the amount of O-glycans with sialic acids in rGth-hCTPs was higher than that in the corresponding rGth-eCTPs.
33539901	2	105	dep	rGth-hCTPs	477:486	arg1	rGth-eCTPs					513:522	rGth-eCTPs	513:522	rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP	477:546	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	2	105	dep	rGth-hCTPs	477:486	arg1	rLh-hCTP					503:510	rLh-hCTP	503:510	rLh-hCTP	503:510	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	2	105	dep	rGth-hCTPs	477:486	arg1	rLh-eCTP					539:546	rLh-eCTP	539:546	rLh-eCTP	539:546	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	2	105	dep	rGth-hCTPs	477:486	arg1	rFsh-eCTP					525:533	rFsh-eCTP	525:533	rFsh-eCTP	525:533	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	2	105	dep	rGth-hCTPs	477:486	arg1	rFsh-hCTP					489:497	rFsh-hCTP	489:497	rFsh-hCTP	489:497	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	1	106	theme	vertebrates	353:363	arg1	biology					342:348	the reproductive biology	325:348	the reproductive biology of vertebrates	325:363	Gonadotropins (Gths), follicle-stimulating hormone (Fsh), and luteinizing hormone (Lh) play central roles in the reproductive biology of vertebrates.
33539901	8	107	from	indices	1833:1839	arg1	eels					1844:1847	eels	1844:1847	eels treated with rGth-hCTPs	1844:1871	The long-term treatment of male eels with these recombinant Gths also revealed the superiority of rGth-hCTPs in assisted reproduction; thus, the serum levels of 11KT and gonadosomatic indices in eels treated with rGth-hCTPs were higher than those in eels treated with the corresponding rGths and rGth-eCTPs.
33539901	9	108	theme	spermatogenesis	1996:2010	arg1	process					1985:1991	the entire process	1974:1991	the entire process of spermatogenesis	1974:2010	The induction of the entire process of spermatogenesis was only histologically observed in rGth-hCTPs-treated eels.
33539901	0	109	theme	chorionic	35:43	arg1	gonadotropin					45:56	human chorionic gonadotropin	29:56	human chorionic gonadotropin	29:56	C-terminal peptide (hCTP) of human chorionic gonadotropin enhances in vivo biological activity of recombinant Japanese eel follicle-stimulating hormone and luteinizing hormone produced in FreeStyle 293-F cell lines.
33539901	7	110	theme	rGth-hCTPs	1459:1468	arg1	levels					1449:1454	The levels	1445:1454	The levels of rGth-hCTPs	1445:1468	The levels of rGth-hCTPs tended to be higher than those of the corresponding rGths and rGth-eCTPs throughout the experimental period, coinciding with the serum fluctuations of 11-ketotestosterone (11KT).
33539901	7	110	theme	rGth-hCTPs	1459:1468	arg1	higher					1483:1488	higher	1483:1488	higher	1483:1488	The levels of rGth-hCTPs tended to be higher than those of the corresponding rGths and rGth-eCTPs throughout the experimental period, coinciding with the serum fluctuations of 11-ketotestosterone (11KT).
33539901	3	111	theme	mannose	877:883	arg1	N-glycans					855:863	N-glycans	855:863	N-glycans of the high mannose and complex types	855:901	Lectin blot analyses demonstrated that all these recombinant Gths contained N-glycans of the high mannose and complex types.
33539901	10	112	theme	sialylated	2240:2249	arg1	antennae					2251:2258	sialylated antennae	2240:2258	sialylated antennae	2240:2258	These findings strongly suggest that hCTP enhances the in vivo biological activity of recombinant Japanese eel Gths due to the high abundance of O-linked glycans with sialylated antennae.
33539901	10	113	theme	in	2128:2129	arg1	activity					2147:2154	the in vivo biological activity	2124:2154	the in vivo biological activity of recombinant Japanese eel Gths	2124:2187	These findings strongly suggest that hCTP enhances the in vivo biological activity of recombinant Japanese eel Gths due to the high abundance of O-linked glycans with sialylated antennae.
33539901	5	114	theme	recombinant	1171:1181	arg1	Fshs					1183:1186	recombinant Fshs	1171:1186	recombinant Fshs	1171:1186	Further analyses of glycans by liquid chromatography-mass spectrometry suggested that the species, amount, and degree of sialylation of N-glycans were comparable among recombinant Fshs and recombinant Lhs, while the amount of O-glycans with sialic acids in rGth-hCTPs was higher than that in the corresponding rGth-eCTPs.
33539901	2	115	theme	HEK293	745:750	arg1	cells					752:756	HEK293 cells	745:756	HEK293 cells	745:756	In this study, recombinant single-chain Japanese eel Gths (rGth: rFsh and rLh), and recombinant chimeric Gths (rGth-hCTPs: rFsh-hCTP and rLh-hCTP; rGth-eCTPs: rFsh-eCTP and rLh-eCTP) with an extra O-glycosylation site (either a C-terminal peptide of human (hCTP) or equine (eCTP) chorionic gonadotropin), which are known to prolong the half-life of glycoprotein were produced in HEK293 cells and highly purified.
33539901	10	116	link	O-linked	2218:2225	arg1	glycans					2227:2233	O-linked glycans	2218:2233	O-linked glycans with sialylated antennae	2218:2258	These findings strongly suggest that hCTP enhances the in vivo biological activity of recombinant Japanese eel Gths due to the high abundance of O-linked glycans with sialylated antennae.
33539901	9	117	theme	rGth-hCTPs-treated	2048:2065	arg1	eels					2067:2070	rGth-hCTPs-treated eels	2048:2070	rGth-hCTPs-treated eels	2048:2070	The induction of the entire process of spermatogenesis was only histologically observed in rGth-hCTPs-treated eels.
33539901	1	118	theme	follicle-stimulating	238:257	arg1	Fsh					268:270	Fsh	268:270	Fsh	268:270	Gonadotropins (Gths), follicle-stimulating hormone (Fsh), and luteinizing hormone (Lh) play central roles in the reproductive biology of vertebrates.
33539901	1	118	theme	follicle-stimulating	238:257	arg1	hormone					259:265	follicle-stimulating hormone	238:265	follicle-stimulating hormone (Fsh)	238:271	Gonadotropins (Gths), follicle-stimulating hormone (Fsh), and luteinizing hormone (Lh) play central roles in the reproductive biology of vertebrates.
34225711	2	0	theme	gut	462:464	arg1	microbes					466:473	the gut microbes	458:473	the gut microbes	458:473	To alleviate LI, previous studies have mainly focused on strengthening intestinal β-galactosidase activity while neglecting the inconspicuous drop in the colon pH caused by the fermentation of non-hydrolyzed lactose by the gut microbes.
34225711	4	1	theme	rescue	693:698	arg1	ability					700:706	pH rescue ability	690:706	pH rescue ability	690:706	RESULTS Here, we synthesized a tri-stable-switch circuit equipped with high β-galactosidase activity and pH rescue ability.
34225711	2	2	theme	β-galactosidase	321:335	arg1	activity					337:344	intestinal β-galactosidase activity	310:344	intestinal β-galactosidase activity	310:344	To alleviate LI, previous studies have mainly focused on strengthening intestinal β-galactosidase activity while neglecting the inconspicuous drop in the colon pH caused by the fermentation of non-hydrolyzed lactose by the gut microbes.
34225711	6	3	theme	pH	1012:1013	arg1	levels					1015:1020	pH levels	1012:1020	pH levels	1012:1020	We confirmed that the circuit functionality was efficient in bacterial cultures at a range of pH levels, and in preventing a drop in pH and β-galactosidase activity after lactose administration to mice.
34225711	2	4	theme	intestinal	310:319	arg1	activity					337:344	intestinal β-galactosidase activity	310:344	intestinal β-galactosidase activity	310:344	To alleviate LI, previous studies have mainly focused on strengthening intestinal β-galactosidase activity while neglecting the inconspicuous drop in the colon pH caused by the fermentation of non-hydrolyzed lactose by the gut microbes.
34225711	6	5	theme	lactose	1089:1095	arg1	administration					1097:1110	lactose administration	1089:1110	lactose administration to mice	1089:1118	We confirmed that the circuit functionality was efficient in bacterial cultures at a range of pH levels, and in preventing a drop in pH and β-galactosidase activity after lactose administration to mice.
34225711	3	6	from	drop	478:481	arg1	pH					492:493	colon pH	486:493	colon pH	486:493	A drop in colon pH will reduce the intestinal β-galactosidase activity and influence intestinal homeostasis.
34225711	8	7	theme	tri-stable-switch	1373:1389	arg1	prototype					1424:1432	a promising prototype	1412:1432	a promising prototype for the relief of lactose intolerance	1412:1470	CONCLUSIONS Due to its ability to flexibly adapt to environmental variation, in particular to stabilize colon pH and maintain β-galactosidase activity after lactose influx, the tri-stable-switch circuit can serve as a promising prototype for the relief of lactose intolerance.
34225711	8	7	theme	tri-stable-switch	1373:1389	arg1	CONCLUSIONS					1196:1206	CONCLUSIONS	1196:1206	CONCLUSIONS Due to its ability to flexibly adapt to environmental variation	1196:1270	CONCLUSIONS Due to its ability to flexibly adapt to environmental variation, in particular to stabilize colon pH and maintain β-galactosidase activity after lactose influx, the tri-stable-switch circuit can serve as a promising prototype for the relief of lactose intolerance.
34225711	8	7	theme	tri-stable-switch	1373:1389	arg1	circuit					1391:1397	the tri-stable-switch circuit	1369:1397	the tri-stable-switch circuit	1369:1397	CONCLUSIONS Due to its ability to flexibly adapt to environmental variation, in particular to stabilize colon pH and maintain β-galactosidase activity after lactose influx, the tri-stable-switch circuit can serve as a promising prototype for the relief of lactose intolerance.
34225711	7	8	theme	circuit	1138:1144	arg1	impact					1124:1129	An impact	1121:1129	An impact of the circuit on gut microbiota composition	1121:1174	An impact of the circuit on gut microbiota composition was also indicated.
34225711	1	9	theme	BACKGROUND	67:76	arg1	malabsorption					86:98	BACKGROUND Lactose malabsorption	67:98	BACKGROUND Lactose malabsorption	67:98	BACKGROUND Lactose malabsorption occurs in around 68% of the world's population, causing lactose intolerance (LI) symptoms, such as abdominal pain, bloating, and diarrhea.
34225711	4	10	theme	pH	690:691	arg1	ability					700:706	pH rescue ability	690:706	pH rescue ability	690:706	RESULTS Here, we synthesized a tri-stable-switch circuit equipped with high β-galactosidase activity and pH rescue ability.
34225711	6	11	from	range	1003:1007	arg1	efficient					966:974	efficient	966:974	efficient	966:974	We confirmed that the circuit functionality was efficient in bacterial cultures at a range of pH levels, and in preventing a drop in pH and β-galactosidase activity after lactose administration to mice.
34225711	6	11	from	range	1003:1007	arg1	functionality					948:960	the circuit functionality	936:960	the circuit functionality	936:960	We confirmed that the circuit functionality was efficient in bacterial cultures at a range of pH levels, and in preventing a drop in pH and β-galactosidase activity after lactose administration to mice.
34225711	5	12	theme	L-lactate	810:818	arg1	dehydrogenase					820:832	L-lactate dehydrogenase	810:832	L-lactate dehydrogenase	810:832	This circuit can switch in functionality between the expression of β-galactosidase and expression of L-lactate dehydrogenase in response to an intestinal lactose signal and intestinal pH signal, respectively.
34225711	1	13	theme	Lactose	78:84	arg1	malabsorption					86:98	BACKGROUND Lactose malabsorption	67:98	BACKGROUND Lactose malabsorption	67:98	BACKGROUND Lactose malabsorption occurs in around 68% of the world's population, causing lactose intolerance (LI) symptoms, such as abdominal pain, bloating, and diarrhea.
34225711	5	14	theme	intestinal	882:891	arg1	signal					896:901	intestinal pH signal	882:901	intestinal pH signal	882:901	This circuit can switch in functionality between the expression of β-galactosidase and expression of L-lactate dehydrogenase in response to an intestinal lactose signal and intestinal pH signal, respectively.
34225711	1	15	theme	lactose	156:162	arg1	LI					177:178	LI	177:178	LI	177:178	BACKGROUND Lactose malabsorption occurs in around 68% of the world's population, causing lactose intolerance (LI) symptoms, such as abdominal pain, bloating, and diarrhea.
34225711	1	15	theme	lactose	156:162	arg1	intolerance					164:174	lactose intolerance	156:174	lactose intolerance (LI) symptoms	156:188	BACKGROUND Lactose malabsorption occurs in around 68% of the world's population, causing lactose intolerance (LI) symptoms, such as abdominal pain, bloating, and diarrhea.
34225711	5	16	theme	β-galactosidase	776:790	arg1	expression					762:771	the expression	758:771	the expression of β-galactosidase and expression of L-lactate dehydrogenase in response to an intestinal lactose signal and intestinal pH signal	758:901	This circuit can switch in functionality between the expression of β-galactosidase and expression of L-lactate dehydrogenase in response to an intestinal lactose signal and intestinal pH signal, respectively.
34225711	8	17	theme	lactose	1353:1359	arg1	influx					1361:1366	lactose influx	1353:1366	lactose influx	1353:1366	CONCLUSIONS Due to its ability to flexibly adapt to environmental variation, in particular to stabilize colon pH and maintain β-galactosidase activity after lactose influx, the tri-stable-switch circuit can serve as a promising prototype for the relief of lactose intolerance.
34225711	1	18	theme	intolerance	164:174	arg1	pain					209:212	abdominal pain	199:212	abdominal pain	199:212	BACKGROUND Lactose malabsorption occurs in around 68% of the world's population, causing lactose intolerance (LI) symptoms, such as abdominal pain, bloating, and diarrhea.
34225711	1	18	theme	intolerance	164:174	arg1	symptoms					181:188	lactose intolerance (LI) symptoms	156:188	lactose intolerance (LI) symptoms	156:188	BACKGROUND Lactose malabsorption occurs in around 68% of the world's population, causing lactose intolerance (LI) symptoms, such as abdominal pain, bloating, and diarrhea.
34225711	1	18	theme	intolerance	164:174	arg1	bloating					215:222	bloating	215:222	bloating	215:222	BACKGROUND Lactose malabsorption occurs in around 68% of the world's population, causing lactose intolerance (LI) symptoms, such as abdominal pain, bloating, and diarrhea.
34225711	1	18	theme	intolerance	164:174	arg1	diarrhea					229:236	diarrhea	229:236	diarrhea	229:236	BACKGROUND Lactose malabsorption occurs in around 68% of the world's population, causing lactose intolerance (LI) symptoms, such as abdominal pain, bloating, and diarrhea.
34225711	0	19	theme	engineered	3:12	arg1	circuit					22:28	An engineered genetic circuit	0:28	An engineered genetic circuit for lactose intolerance alleviation.	0:65	An engineered genetic circuit for lactose intolerance alleviation.
34225711	6	20	from	preventing	1030:1039	arg1	efficient					966:974	efficient	966:974	efficient	966:974	We confirmed that the circuit functionality was efficient in bacterial cultures at a range of pH levels, and in preventing a drop in pH and β-galactosidase activity after lactose administration to mice.
34225711	6	20	from	preventing	1030:1039	arg1	functionality					948:960	the circuit functionality	936:960	the circuit functionality	936:960	We confirmed that the circuit functionality was efficient in bacterial cultures at a range of pH levels, and in preventing a drop in pH and β-galactosidase activity after lactose administration to mice.
34225711	4	21	theme	β-galactosidase	661:675	arg1	activity					677:684	high β-galactosidase activity	656:684	high β-galactosidase activity	656:684	RESULTS Here, we synthesized a tri-stable-switch circuit equipped with high β-galactosidase activity and pH rescue ability.
34225711	5	22	theme	dehydrogenase	820:832	arg1	expression					796:805	expression	796:805	expression	796:805	This circuit can switch in functionality between the expression of β-galactosidase and expression of L-lactate dehydrogenase in response to an intestinal lactose signal and intestinal pH signal, respectively.
34225711	5	22	theme	dehydrogenase	820:832	arg1	β-galactosidase					776:790	β-galactosidase	776:790	β-galactosidase	776:790	This circuit can switch in functionality between the expression of β-galactosidase and expression of L-lactate dehydrogenase in response to an intestinal lactose signal and intestinal pH signal, respectively.
34225711	8	23	theme	environmental	1248:1260	arg1	variation					1262:1270	environmental variation	1248:1270	environmental variation	1248:1270	CONCLUSIONS Due to its ability to flexibly adapt to environmental variation, in particular to stabilize colon pH and maintain β-galactosidase activity after lactose influx, the tri-stable-switch circuit can serve as a promising prototype for the relief of lactose intolerance.
34225711	4	24	theme	high	656:659	arg1	activity					677:684	high β-galactosidase activity	656:684	high β-galactosidase activity	656:684	RESULTS Here, we synthesized a tri-stable-switch circuit equipped with high β-galactosidase activity and pH rescue ability.
34225711	7	25	theme	gut	1149:1151	arg1	composition					1164:1174	gut microbiota composition	1149:1174	gut microbiota composition	1149:1174	An impact of the circuit on gut microbiota composition was also indicated.
34225711	6	26	theme	β-galactosidase	1058:1072	arg1	activity					1074:1081	β-galactosidase activity	1058:1081	β-galactosidase activity	1058:1081	We confirmed that the circuit functionality was efficient in bacterial cultures at a range of pH levels, and in preventing a drop in pH and β-galactosidase activity after lactose administration to mice.
34225711	8	27	theme	lactose	1452:1458	arg1	intolerance					1460:1470	lactose intolerance	1452:1470	lactose intolerance	1452:1470	CONCLUSIONS Due to its ability to flexibly adapt to environmental variation, in particular to stabilize colon pH and maintain β-galactosidase activity after lactose influx, the tri-stable-switch circuit can serve as a promising prototype for the relief of lactose intolerance.
34225711	6	28	from	drop	1043:1046	arg1	pH					1051:1052	pH	1051:1052	pH	1051:1052	We confirmed that the circuit functionality was efficient in bacterial cultures at a range of pH levels, and in preventing a drop in pH and β-galactosidase activity after lactose administration to mice.
34225711	6	28	from	drop	1043:1046	arg1	activity					1074:1081	β-galactosidase activity	1058:1081	β-galactosidase activity	1058:1081	We confirmed that the circuit functionality was efficient in bacterial cultures at a range of pH levels, and in preventing a drop in pH and β-galactosidase activity after lactose administration to mice.
34225711	2	29	theme	previous	256:263	arg1	studies					265:271	previous studies	256:271	previous studies	256:271	To alleviate LI, previous studies have mainly focused on strengthening intestinal β-galactosidase activity while neglecting the inconspicuous drop in the colon pH caused by the fermentation of non-hydrolyzed lactose by the gut microbes.
34225711	0	30	theme	genetic	14:20	arg1	circuit					22:28	An engineered genetic circuit	0:28	An engineered genetic circuit for lactose intolerance alleviation.	0:65	An engineered genetic circuit for lactose intolerance alleviation.
34225711	6	31	theme	bacterial	979:987	arg1	cultures					989:996	bacterial cultures	979:996	bacterial cultures	979:996	We confirmed that the circuit functionality was efficient in bacterial cultures at a range of pH levels, and in preventing a drop in pH and β-galactosidase activity after lactose administration to mice.
34225711	3	32	theme	β-galactosidase	522:536	arg1	activity					538:545	the intestinal β-galactosidase activity	507:545	the intestinal β-galactosidase activity	507:545	A drop in colon pH will reduce the intestinal β-galactosidase activity and influence intestinal homeostasis.
34225711	2	33	theme	colon	393:397	arg1	pH					399:400	the colon pH	389:400	the colon pH caused by the fermentation of non-hydrolyzed lactose by the gut microbes	389:473	To alleviate LI, previous studies have mainly focused on strengthening intestinal β-galactosidase activity while neglecting the inconspicuous drop in the colon pH caused by the fermentation of non-hydrolyzed lactose by the gut microbes.
34225711	0	34	theme	lactose	34:40	arg1	alleviation					54:64	lactose intolerance alleviation	34:64	lactose intolerance alleviation	34:64	An engineered genetic circuit for lactose intolerance alleviation.
34225711	5	35	theme	pH	893:894	arg1	signal					896:901	intestinal pH signal	882:901	intestinal pH signal	882:901	This circuit can switch in functionality between the expression of β-galactosidase and expression of L-lactate dehydrogenase in response to an intestinal lactose signal and intestinal pH signal, respectively.
34225711	5	36	theme	intestinal	852:861	arg1	signal					871:876	an intestinal lactose signal	849:876	an intestinal lactose signal	849:876	This circuit can switch in functionality between the expression of β-galactosidase and expression of L-lactate dehydrogenase in response to an intestinal lactose signal and intestinal pH signal, respectively.
34225711	2	37	theme	lactose	447:453	arg1	fermentation					416:427	the fermentation	412:427	the fermentation of non-hydrolyzed lactose	412:453	To alleviate LI, previous studies have mainly focused on strengthening intestinal β-galactosidase activity while neglecting the inconspicuous drop in the colon pH caused by the fermentation of non-hydrolyzed lactose by the gut microbes.
34225711	6	38	from	cultures	989:996	arg1	efficient					966:974	efficient	966:974	efficient	966:974	We confirmed that the circuit functionality was efficient in bacterial cultures at a range of pH levels, and in preventing a drop in pH and β-galactosidase activity after lactose administration to mice.
34225711	6	38	from	cultures	989:996	arg1	functionality					948:960	the circuit functionality	936:960	the circuit functionality	936:960	We confirmed that the circuit functionality was efficient in bacterial cultures at a range of pH levels, and in preventing a drop in pH and β-galactosidase activity after lactose administration to mice.
34225711	8	39	theme	intolerance	1460:1470	arg1	relief					1442:1447	the relief	1438:1447	the relief of lactose intolerance	1438:1470	CONCLUSIONS Due to its ability to flexibly adapt to environmental variation, in particular to stabilize colon pH and maintain β-galactosidase activity after lactose influx, the tri-stable-switch circuit can serve as a promising prototype for the relief of lactose intolerance.
34225711	5	40	theme	lactose	863:869	arg1	signal					871:876	an intestinal lactose signal	849:876	an intestinal lactose signal	849:876	This circuit can switch in functionality between the expression of β-galactosidase and expression of L-lactate dehydrogenase in response to an intestinal lactose signal and intestinal pH signal, respectively.
34225711	4	41	theme	tri-stable-switch	616:632	arg1	circuit					634:640	a tri-stable-switch circuit	614:640	a tri-stable-switch circuit equipped with high β-galactosidase activity and pH rescue ability	614:706	RESULTS Here, we synthesized a tri-stable-switch circuit equipped with high β-galactosidase activity and pH rescue ability.
34225711	2	42	theme	non-hydrolyzed	432:445	arg1	lactose					447:453	non-hydrolyzed lactose	432:453	non-hydrolyzed lactose	432:453	To alleviate LI, previous studies have mainly focused on strengthening intestinal β-galactosidase activity while neglecting the inconspicuous drop in the colon pH caused by the fermentation of non-hydrolyzed lactose by the gut microbes.
34225711	8	43	theme	Due	1208:1210	arg1	prototype					1424:1432	a promising prototype	1412:1432	a promising prototype for the relief of lactose intolerance	1412:1470	CONCLUSIONS Due to its ability to flexibly adapt to environmental variation, in particular to stabilize colon pH and maintain β-galactosidase activity after lactose influx, the tri-stable-switch circuit can serve as a promising prototype for the relief of lactose intolerance.
34225711	8	43	theme	Due	1208:1210	arg1	CONCLUSIONS					1196:1206	CONCLUSIONS	1196:1206	CONCLUSIONS Due to its ability to flexibly adapt to environmental variation	1196:1270	CONCLUSIONS Due to its ability to flexibly adapt to environmental variation, in particular to stabilize colon pH and maintain β-galactosidase activity after lactose influx, the tri-stable-switch circuit can serve as a promising prototype for the relief of lactose intolerance.
34225711	8	43	theme	Due	1208:1210	arg1	circuit					1391:1397	the tri-stable-switch circuit	1369:1397	the tri-stable-switch circuit	1369:1397	CONCLUSIONS Due to its ability to flexibly adapt to environmental variation, in particular to stabilize colon pH and maintain β-galactosidase activity after lactose influx, the tri-stable-switch circuit can serve as a promising prototype for the relief of lactose intolerance.
34225711	7	44	from	impact	1124:1129	arg1	composition					1164:1174	gut microbiota composition	1149:1174	gut microbiota composition	1149:1174	An impact of the circuit on gut microbiota composition was also indicated.
34225711	0	45	theme	intolerance	42:52	arg1	alleviation					54:64	lactose intolerance alleviation	34:64	lactose intolerance alleviation	34:64	An engineered genetic circuit for lactose intolerance alleviation.
34225711	5	46	from	expression	762:771	arg1	response					837:844	response	837:844	response to an intestinal lactose signal and intestinal pH signal	837:901	This circuit can switch in functionality between the expression of β-galactosidase and expression of L-lactate dehydrogenase in response to an intestinal lactose signal and intestinal pH signal, respectively.
34225711	3	47	theme	intestinal	561:570	arg1	homeostasis					572:582	intestinal homeostasis	561:582	intestinal homeostasis	561:582	A drop in colon pH will reduce the intestinal β-galactosidase activity and influence intestinal homeostasis.
34225711	2	48	from	drop	381:384	arg1	pH					399:400	the colon pH	389:400	the colon pH caused by the fermentation of non-hydrolyzed lactose by the gut microbes	389:473	To alleviate LI, previous studies have mainly focused on strengthening intestinal β-galactosidase activity while neglecting the inconspicuous drop in the colon pH caused by the fermentation of non-hydrolyzed lactose by the gut microbes.
34225711	6	49	theme	circuit	940:946	arg1	efficient					966:974	efficient	966:974	efficient	966:974	We confirmed that the circuit functionality was efficient in bacterial cultures at a range of pH levels, and in preventing a drop in pH and β-galactosidase activity after lactose administration to mice.
34225711	6	49	theme	circuit	940:946	arg1	functionality					948:960	the circuit functionality	936:960	the circuit functionality	936:960	We confirmed that the circuit functionality was efficient in bacterial cultures at a range of pH levels, and in preventing a drop in pH and β-galactosidase activity after lactose administration to mice.
34225711	6	50	from	efficient	966:974	arg1	cultures					989:996	bacterial cultures	979:996	bacterial cultures	979:996	We confirmed that the circuit functionality was efficient in bacterial cultures at a range of pH levels, and in preventing a drop in pH and β-galactosidase activity after lactose administration to mice.
34225711	8	51	theme	colon	1300:1304	arg1	pH					1306:1307	colon pH	1300:1307	colon pH	1300:1307	CONCLUSIONS Due to its ability to flexibly adapt to environmental variation, in particular to stabilize colon pH and maintain β-galactosidase activity after lactose influx, the tri-stable-switch circuit can serve as a promising prototype for the relief of lactose intolerance.
34225711	3	52	theme	intestinal	511:520	arg1	activity					538:545	the intestinal β-galactosidase activity	507:545	the intestinal β-galactosidase activity	507:545	A drop in colon pH will reduce the intestinal β-galactosidase activity and influence intestinal homeostasis.
34225711	8	53	theme	promising	1414:1422	arg1	prototype					1424:1432	a promising prototype	1412:1432	a promising prototype for the relief of lactose intolerance	1412:1470	CONCLUSIONS Due to its ability to flexibly adapt to environmental variation, in particular to stabilize colon pH and maintain β-galactosidase activity after lactose influx, the tri-stable-switch circuit can serve as a promising prototype for the relief of lactose intolerance.
34225711	8	53	theme	promising	1414:1422	arg1	CONCLUSIONS					1196:1206	CONCLUSIONS	1196:1206	CONCLUSIONS Due to its ability to flexibly adapt to environmental variation	1196:1270	CONCLUSIONS Due to its ability to flexibly adapt to environmental variation, in particular to stabilize colon pH and maintain β-galactosidase activity after lactose influx, the tri-stable-switch circuit can serve as a promising prototype for the relief of lactose intolerance.
34225711	8	53	theme	promising	1414:1422	arg1	circuit					1391:1397	the tri-stable-switch circuit	1369:1397	the tri-stable-switch circuit	1369:1397	CONCLUSIONS Due to its ability to flexibly adapt to environmental variation, in particular to stabilize colon pH and maintain β-galactosidase activity after lactose influx, the tri-stable-switch circuit can serve as a promising prototype for the relief of lactose intolerance.
34225711	1	54	theme	population	136:145	arg1	population					136:145	the world's population	124:145	the world's population	124:145	BACKGROUND Lactose malabsorption occurs in around 68% of the world's population, causing lactose intolerance (LI) symptoms, such as abdominal pain, bloating, and diarrhea.
34225711	1	54	theme	population	136:145	arg1	%					119:119	68%	117:119	68% of the world's population	117:145	BACKGROUND Lactose malabsorption occurs in around 68% of the world's population, causing lactose intolerance (LI) symptoms, such as abdominal pain, bloating, and diarrhea.
34225711	8	55	theme	β-galactosidase	1322:1336	arg1	activity					1338:1345	β-galactosidase activity	1322:1345	β-galactosidase activity	1322:1345	CONCLUSIONS Due to its ability to flexibly adapt to environmental variation, in particular to stabilize colon pH and maintain β-galactosidase activity after lactose influx, the tri-stable-switch circuit can serve as a promising prototype for the relief of lactose intolerance.
34225711	1	56	theme	abdominal	199:207	arg1	pain					209:212	abdominal pain	199:212	abdominal pain	199:212	BACKGROUND Lactose malabsorption occurs in around 68% of the world's population, causing lactose intolerance (LI) symptoms, such as abdominal pain, bloating, and diarrhea.
34225711	4	57	dep	RESULTS	585:591	arg1	synthesized					602:612	synthesized	602:612	synthesized a tri-stable-switch circuit equipped with high β-galactosidase activity and pH rescue ability	602:706	RESULTS Here, we synthesized a tri-stable-switch circuit equipped with high β-galactosidase activity and pH rescue ability.
34225711	2	58	theme	inconspicuous	367:379	arg1	drop					381:384	the inconspicuous drop	363:384	the inconspicuous drop in the colon pH caused by the fermentation of non-hydrolyzed lactose by the gut microbes	363:473	To alleviate LI, previous studies have mainly focused on strengthening intestinal β-galactosidase activity while neglecting the inconspicuous drop in the colon pH caused by the fermentation of non-hydrolyzed lactose by the gut microbes.
34225711	5	59	theme	expression	796:805	arg1	expression					762:771	the expression	758:771	the expression of β-galactosidase and expression of L-lactate dehydrogenase in response to an intestinal lactose signal and intestinal pH signal	758:901	This circuit can switch in functionality between the expression of β-galactosidase and expression of L-lactate dehydrogenase in response to an intestinal lactose signal and intestinal pH signal, respectively.
34225711	7	60	theme	microbiota	1153:1162	arg1	composition					1164:1174	gut microbiota composition	1149:1174	gut microbiota composition	1149:1174	An impact of the circuit on gut microbiota composition was also indicated.
34225711	3	61	theme	colon	486:490	arg1	pH					492:493	colon pH	486:493	colon pH	486:493	A drop in colon pH will reduce the intestinal β-galactosidase activity and influence intestinal homeostasis.
34225711	6	62	theme	levels	1015:1020	arg1	range					1003:1007	a range	1001:1007	a range of pH levels	1001:1020	We confirmed that the circuit functionality was efficient in bacterial cultures at a range of pH levels, and in preventing a drop in pH and β-galactosidase activity after lactose administration to mice.
34896151	7	0	from	level	1114:1118	arg1	group					1134:1138	the normal group	1123:1138	the normal group	1123:1138	Meanwhile, SCFAs produced by gut microbiota were restored by sCPA close to the level in the normal group.
34896151	8	1	theme	selenium	1224:1231	arg1	supplementation					1233:1247	a potential dietary selenium supplementation	1204:1247	a potential dietary selenium supplementation for the prevention and treatment of colitis	1204:1291	In conclusion, these findings indicated that the sCPA might be a potential dietary selenium supplementation for the prevention and treatment of colitis.
34896151	8	1	theme	selenium	1224:1231	arg1	sCPA					1190:1193	the sCPA	1186:1193	the sCPA	1186:1193	In conclusion, these findings indicated that the sCPA might be a potential dietary selenium supplementation for the prevention and treatment of colitis.
34896151	1	2	theme	colitis	254:260	arg1	treatment					262:270	colitis treatment	254:270	colitis treatment	254:270	The deficiency of selenium has been found in clinical IBD patients and supplementation selenium is recognized as beneficial for colitis treatment.
34896151	0	3	theme	sulfate	86:92	arg1	sodium					94:99	dextran sulfate sodium	78:99	dextran sulfate sodium induced colitis in vivo	78:123	Supplementary selenium in the form of selenylation α-D-1,6-glucan ameliorates dextran sulfate sodium induced colitis in vivo.
34896151	0	4	from	selenium	14:21	arg1	form					30:33	the form	26:33	the form of selenylation α-D-1,6-glucan	26:64	Supplementary selenium in the form of selenylation α-D-1,6-glucan ameliorates dextran sulfate sodium induced colitis in vivo.
34896151	3	5	theme	colon	494:498	arg1	shortening					507:516	colon length shortening	494:516	colon length shortening	494:516	The results suggested that sCPA prevented the weight loss, colon length shortening, and stool loose of colitis mice.
34896151	3	6	theme	length	500:505	arg1	shortening					507:516	colon length shortening	494:516	colon length shortening	494:516	The results suggested that sCPA prevented the weight loss, colon length shortening, and stool loose of colitis mice.
34896151	3	7	theme	stool	523:527	arg1	loose					529:533	stool loose	523:533	stool loose	523:533	The results suggested that sCPA prevented the weight loss, colon length shortening, and stool loose of colitis mice.
34896151	3	8	theme	colitis	538:544	arg1	mice					546:549	colitis mice	538:549	colitis mice	538:549	The results suggested that sCPA prevented the weight loss, colon length shortening, and stool loose of colitis mice.
34896151	4	9	theme	mucosal	571:577	arg1	barrier					579:585	colon mucosal barrier	565:585	colon mucosal barrier	565:585	It protected colon mucosal barrier by promoting tight junction protein ZO-1 and Occludin expression.
34896151	5	10	theme	MDA	716:718	arg1	levels					720:725	SOD and MDA levels	708:725	SOD and MDA levels	708:725	Moreover, sCPA reduced oxidative stress via regulating SOD and MDA levels, and decreased the contents of inflammatory proteins NF-κB and NLRP3 and adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines.
34896151	4	11	theme	junction	606:613	arg1	ZO-1					623:626	tight junction protein ZO-1	600:626	tight junction protein ZO-1	600:626	It protected colon mucosal barrier by promoting tight junction protein ZO-1 and Occludin expression.
34896151	4	12	theme	colon	565:569	arg1	barrier					579:585	colon mucosal barrier	565:585	colon mucosal barrier	565:585	It protected colon mucosal barrier by promoting tight junction protein ZO-1 and Occludin expression.
34896151	5	13	dep	proteins	771:778	arg1	proteins					771:778	inflammatory proteins NF-κB and NLRP3	758:794	inflammatory proteins NF-κB and NLRP3	758:794	Moreover, sCPA reduced oxidative stress via regulating SOD and MDA levels, and decreased the contents of inflammatory proteins NF-κB and NLRP3 and adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines.
34896151	5	13	dep	proteins	771:778	arg1	NLRP3					790:794	NLRP3	790:794	NLRP3	790:794	Moreover, sCPA reduced oxidative stress via regulating SOD and MDA levels, and decreased the contents of inflammatory proteins NF-κB and NLRP3 and adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines.
34896151	5	13	dep	proteins	771:778	arg1	NF-κB					780:784	NF-κB	780:784	NF-κB	780:784	Moreover, sCPA reduced oxidative stress via regulating SOD and MDA levels, and decreased the contents of inflammatory proteins NF-κB and NLRP3 and adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines.
34896151	2	14	theme	colitis	404:410	arg1	mice					412:415	DSS induced colitis mice	392:415	DSS induced colitis mice	392:415	In this study, an organic selenium compound-selenylation α-D-1,6-glucan (sCPA) was prepared, and the effect of sCPA on DSS induced colitis mice was investigated.
34896151	8	15	theme	potential	1206:1214	arg1	supplementation					1233:1247	a potential dietary selenium supplementation	1204:1247	a potential dietary selenium supplementation for the prevention and treatment of colitis	1204:1291	In conclusion, these findings indicated that the sCPA might be a potential dietary selenium supplementation for the prevention and treatment of colitis.
34896151	8	15	theme	potential	1206:1214	arg1	sCPA					1190:1193	the sCPA	1186:1193	the sCPA	1186:1193	In conclusion, these findings indicated that the sCPA might be a potential dietary selenium supplementation for the prevention and treatment of colitis.
34896151	5	16	theme	IL-1β	823:827	arg1	cytokines					853:861	adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines	800:861	adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines	800:861	Moreover, sCPA reduced oxidative stress via regulating SOD and MDA levels, and decreased the contents of inflammatory proteins NF-κB and NLRP3 and adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines.
34896151	1	17	located	found	162:166	arg1	patients					184:191	clinical IBD patients	171:191	clinical IBD patients	171:191	The deficiency of selenium has been found in clinical IBD patients and supplementation selenium is recognized as beneficial for colitis treatment.
34896151	1	17	located	found	162:166	arg2	deficiency					130:139	The deficiency	126:139	The deficiency of selenium	126:151	The deficiency of selenium has been found in clinical IBD patients and supplementation selenium is recognized as beneficial for colitis treatment.
34896151	3	18	dep	loss	488:491	arg1	the					477:479	the	477:479	the	477:479	The results suggested that sCPA prevented the weight loss, colon length shortening, and stool loose of colitis mice.
34896151	1	19	theme	clinical	171:178	arg1	patients					184:191	clinical IBD patients	171:191	clinical IBD patients	171:191	The deficiency of selenium has been found in clinical IBD patients and supplementation selenium is recognized as beneficial for colitis treatment.
34896151	7	20	theme	gut	1064:1066	arg1	microbiota					1068:1077	gut microbiota	1064:1077	gut microbiota	1064:1077	Meanwhile, SCFAs produced by gut microbiota were restored by sCPA close to the level in the normal group.
34896151	3	21	theme	mice	546:549	arg1	loose					529:533	stool loose	523:533	stool loose	523:533	The results suggested that sCPA prevented the weight loss, colon length shortening, and stool loose of colitis mice.
34896151	3	21	theme	mice	546:549	arg1	loss					488:491	weight loss	481:491	weight loss	481:491	The results suggested that sCPA prevented the weight loss, colon length shortening, and stool loose of colitis mice.
34896151	3	21	theme	mice	546:549	arg1	shortening					507:516	colon length shortening	494:516	colon length shortening	494:516	The results suggested that sCPA prevented the weight loss, colon length shortening, and stool loose of colitis mice.
34896151	1	22	theme	IBD	180:182	arg1	patients					184:191	clinical IBD patients	171:191	clinical IBD patients	171:191	The deficiency of selenium has been found in clinical IBD patients and supplementation selenium is recognized as beneficial for colitis treatment.
34896151	0	23	theme	Supplementary	0:12	arg1	selenium					14:21	Supplementary selenium	0:21	Supplementary selenium in the form of selenylation α-D-1,6-glucan	0:64	Supplementary selenium in the form of selenylation α-D-1,6-glucan ameliorates dextran sulfate sodium induced colitis in vivo.
34896151	2	24	theme	compound-selenylation	308:328	arg1	sCPA					346:349	sCPA	346:349	sCPA	346:349	In this study, an organic selenium compound-selenylation α-D-1,6-glucan (sCPA) was prepared, and the effect of sCPA on DSS induced colitis mice was investigated.
34896151	2	24	theme	compound-selenylation	308:328	arg1	α-D-1,6-glucan					330:343	an organic selenium compound-selenylation α-D-1,6-glucan	288:343	an organic selenium compound-selenylation α-D-1,6-glucan (sCPA)	288:350	In this study, an organic selenium compound-selenylation α-D-1,6-glucan (sCPA) was prepared, and the effect of sCPA on DSS induced colitis mice was investigated.
34896151	8	25	theme	dietary	1216:1222	arg1	supplementation					1233:1247	a potential dietary selenium supplementation	1204:1247	a potential dietary selenium supplementation for the prevention and treatment of colitis	1204:1291	In conclusion, these findings indicated that the sCPA might be a potential dietary selenium supplementation for the prevention and treatment of colitis.
34896151	8	25	theme	dietary	1216:1222	arg1	sCPA					1190:1193	the sCPA	1186:1193	the sCPA	1186:1193	In conclusion, these findings indicated that the sCPA might be a potential dietary selenium supplementation for the prevention and treatment of colitis.
34896151	5	26	theme	IL-10	834:838	arg1	cytokines					853:861	adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines	800:861	adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines	800:861	Moreover, sCPA reduced oxidative stress via regulating SOD and MDA levels, and decreased the contents of inflammatory proteins NF-κB and NLRP3 and adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines.
34896151	7	27	theme	normal	1127:1132	arg1	group					1134:1138	the normal group	1123:1138	the normal group	1123:1138	Meanwhile, SCFAs produced by gut microbiota were restored by sCPA close to the level in the normal group.
34896151	2	28	theme	selenium	299:306	arg1	sCPA					346:349	sCPA	346:349	sCPA	346:349	In this study, an organic selenium compound-selenylation α-D-1,6-glucan (sCPA) was prepared, and the effect of sCPA on DSS induced colitis mice was investigated.
34896151	2	28	theme	selenium	299:306	arg1	α-D-1,6-glucan					330:343	an organic selenium compound-selenylation α-D-1,6-glucan	288:343	an organic selenium compound-selenylation α-D-1,6-glucan (sCPA)	288:350	In this study, an organic selenium compound-selenylation α-D-1,6-glucan (sCPA) was prepared, and the effect of sCPA on DSS induced colitis mice was investigated.
34896151	4	29	theme	protein	615:621	arg1	ZO-1					623:626	tight junction protein ZO-1	600:626	tight junction protein ZO-1	600:626	It protected colon mucosal barrier by promoting tight junction protein ZO-1 and Occludin expression.
34896151	5	30	theme	adjusted	800:807	arg1	cytokines					853:861	adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines	800:861	adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines	800:861	Moreover, sCPA reduced oxidative stress via regulating SOD and MDA levels, and decreased the contents of inflammatory proteins NF-κB and NLRP3 and adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines.
34896151	2	31	theme	organic	291:297	arg1	sCPA					346:349	sCPA	346:349	sCPA	346:349	In this study, an organic selenium compound-selenylation α-D-1,6-glucan (sCPA) was prepared, and the effect of sCPA on DSS induced colitis mice was investigated.
34896151	2	31	theme	organic	291:297	arg1	α-D-1,6-glucan					330:343	an organic selenium compound-selenylation α-D-1,6-glucan	288:343	an organic selenium compound-selenylation α-D-1,6-glucan (sCPA)	288:350	In this study, an organic selenium compound-selenylation α-D-1,6-glucan (sCPA) was prepared, and the effect of sCPA on DSS induced colitis mice was investigated.
34896151	1	32	theme	supplementation	197:211	arg1	selenium					213:220	supplementation selenium	197:220	supplementation selenium	197:220	The deficiency of selenium has been found in clinical IBD patients and supplementation selenium is recognized as beneficial for colitis treatment.
34896151	5	33	theme	IFN-γ	816:820	arg1	cytokines					853:861	adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines	800:861	adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines	800:861	Moreover, sCPA reduced oxidative stress via regulating SOD and MDA levels, and decreased the contents of inflammatory proteins NF-κB and NLRP3 and adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines.
34896151	2	34	theme	induced	396:402	arg1	colitis					404:410	DSS induced colitis	392:410	DSS induced colitis mice	392:415	In this study, an organic selenium compound-selenylation α-D-1,6-glucan (sCPA) was prepared, and the effect of sCPA on DSS induced colitis mice was investigated.
34896151	5	35	theme	inflammatory	758:769	arg1	proteins					771:778	inflammatory proteins NF-κB and NLRP3	758:794	inflammatory proteins NF-κB and NLRP3	758:794	Moreover, sCPA reduced oxidative stress via regulating SOD and MDA levels, and decreased the contents of inflammatory proteins NF-κB and NLRP3 and adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines.
34896151	5	35	theme	inflammatory	758:769	arg1	NLRP3					790:794	NLRP3	790:794	NLRP3	790:794	Moreover, sCPA reduced oxidative stress via regulating SOD and MDA levels, and decreased the contents of inflammatory proteins NF-κB and NLRP3 and adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines.
34896151	5	35	theme	inflammatory	758:769	arg1	NF-κB					780:784	NF-κB	780:784	NF-κB	780:784	Moreover, sCPA reduced oxidative stress via regulating SOD and MDA levels, and decreased the contents of inflammatory proteins NF-κB and NLRP3 and adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines.
34896151	5	36	theme	SOD	708:710	arg1	levels					720:725	SOD and MDA levels	708:725	SOD and MDA levels	708:725	Moreover, sCPA reduced oxidative stress via regulating SOD and MDA levels, and decreased the contents of inflammatory proteins NF-κB and NLRP3 and adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines.
34896151	4	37	dep	ZO-1	623:626	arg1	expression					641:650	expression	641:650	expression	641:650	It protected colon mucosal barrier by promoting tight junction protein ZO-1 and Occludin expression.
34896151	2	38	theme	DSS	392:394	arg1	colitis					404:410	DSS induced colitis	392:410	DSS induced colitis mice	392:415	In this study, an organic selenium compound-selenylation α-D-1,6-glucan (sCPA) was prepared, and the effect of sCPA on DSS induced colitis mice was investigated.
34896151	0	39	theme	α-D-1,6-glucan	51:64	arg1	form					30:33	the form	26:33	the form of selenylation α-D-1,6-glucan	26:64	Supplementary selenium in the form of selenylation α-D-1,6-glucan ameliorates dextran sulfate sodium induced colitis in vivo.
34896151	5	40	theme	inflammatory	840:851	arg1	cytokines					853:861	adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines	800:861	adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines	800:861	Moreover, sCPA reduced oxidative stress via regulating SOD and MDA levels, and decreased the contents of inflammatory proteins NF-κB and NLRP3 and adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines.
34896151	2	41	from	effect	374:379	arg1	mice					412:415	DSS induced colitis mice	392:415	DSS induced colitis mice	392:415	In this study, an organic selenium compound-selenylation α-D-1,6-glucan (sCPA) was prepared, and the effect of sCPA on DSS induced colitis mice was investigated.
34896151	3	42	theme	weight	481:486	arg1	loss					488:491	weight loss	481:491	weight loss	481:491	The results suggested that sCPA prevented the weight loss, colon length shortening, and stool loose of colitis mice.
34896151	0	43	theme	selenylation	38:49	arg1	α-D-1,6-glucan					51:64	selenylation α-D-1,6-glucan	38:64	selenylation α-D-1,6-glucan	38:64	Supplementary selenium in the form of selenylation α-D-1,6-glucan ameliorates dextran sulfate sodium induced colitis in vivo.
34896151	6	44	dep	Firmicutes	951:960	arg1	Bacteroidetes					936:948	Bacteroidetes	936:948	Bacteroidetes	936:948	Furthermore, sCPA repaired intestinal microbiota composition especially Bacteroidetes, Firmicutes, Proteobacteria, and Actinobacteria that altered by DSS in colitis mice.
34896151	6	45	theme	microbiota	902:911	arg1	composition					913:923	intestinal microbiota composition	891:923	intestinal microbiota composition	891:923	Furthermore, sCPA repaired intestinal microbiota composition especially Bacteroidetes, Firmicutes, Proteobacteria, and Actinobacteria that altered by DSS in colitis mice.
34896151	5	46	theme	oxidative	676:684	arg1	stress					686:691	oxidative stress	676:691	oxidative stress	676:691	Moreover, sCPA reduced oxidative stress via regulating SOD and MDA levels, and decreased the contents of inflammatory proteins NF-κB and NLRP3 and adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines.
34896151	8	47	theme	colitis	1285:1291	arg1	treatment					1272:1280	treatment	1272:1280	treatment	1272:1280	In conclusion, these findings indicated that the sCPA might be a potential dietary selenium supplementation for the prevention and treatment of colitis.
34896151	8	47	theme	colitis	1285:1291	arg1	prevention					1257:1266	prevention	1257:1266	prevention	1257:1266	In conclusion, these findings indicated that the sCPA might be a potential dietary selenium supplementation for the prevention and treatment of colitis.
34896151	0	48	theme	dextran	78:84	arg1	sodium					94:99	dextran sulfate sodium	78:99	dextran sulfate sodium induced colitis in vivo	78:123	Supplementary selenium in the form of selenylation α-D-1,6-glucan ameliorates dextran sulfate sodium induced colitis in vivo.
34896151	6	49	theme	colitis	1021:1027	arg1	mice					1029:1032	colitis mice	1021:1032	colitis mice	1021:1032	Furthermore, sCPA repaired intestinal microbiota composition especially Bacteroidetes, Firmicutes, Proteobacteria, and Actinobacteria that altered by DSS in colitis mice.
34896151	8	50	dep	prevention	1257:1266	arg1	the					1253:1255	the	1253:1255	the	1253:1255	In conclusion, these findings indicated that the sCPA might be a potential dietary selenium supplementation for the prevention and treatment of colitis.
34896151	5	51	theme	TNF-α	809:813	arg1	cytokines					853:861	adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines	800:861	adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines	800:861	Moreover, sCPA reduced oxidative stress via regulating SOD and MDA levels, and decreased the contents of inflammatory proteins NF-κB and NLRP3 and adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines.
34896151	1	52	theme	selenium	144:151	arg1	deficiency					130:139	The deficiency	126:139	The deficiency of selenium	126:151	The deficiency of selenium has been found in clinical IBD patients and supplementation selenium is recognized as beneficial for colitis treatment.
34896151	2	53	theme	sCPA	384:387	arg1	effect					374:379	the effect	370:379	the effect of sCPA on DSS induced colitis mice	370:415	In this study, an organic selenium compound-selenylation α-D-1,6-glucan (sCPA) was prepared, and the effect of sCPA on DSS induced colitis mice was investigated.
34896151	4	54	theme	tight	600:604	arg1	ZO-1					623:626	tight junction protein ZO-1	600:626	tight junction protein ZO-1	600:626	It protected colon mucosal barrier by promoting tight junction protein ZO-1 and Occludin expression.
34896151	5	55	theme	proteins	771:778	arg1	cytokines					853:861	adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines	800:861	adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines	800:861	Moreover, sCPA reduced oxidative stress via regulating SOD and MDA levels, and decreased the contents of inflammatory proteins NF-κB and NLRP3 and adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines.
34896151	5	55	theme	proteins	771:778	arg1	contents					746:753	the contents	742:753	the contents of inflammatory proteins NF-κB and NLRP3	742:794	Moreover, sCPA reduced oxidative stress via regulating SOD and MDA levels, and decreased the contents of inflammatory proteins NF-κB and NLRP3 and adjusted TNF-α, IFN-γ, IL-1β, and IL-10 inflammatory cytokines.
34896151	6	56	theme	intestinal	891:900	arg1	composition					913:923	intestinal microbiota composition	891:923	intestinal microbiota composition	891:923	Furthermore, sCPA repaired intestinal microbiota composition especially Bacteroidetes, Firmicutes, Proteobacteria, and Actinobacteria that altered by DSS in colitis mice.
34688330	8	0	theme	high	1133:1136	arg1	rate					1152:1155	high encapsulation rate	1133:1155	high encapsulation rate of 81.26%	1133:1165	At a ratio of 2:1 chitosan and TGF-β1, the particle size was102.65±11.98 nm, which was smallest, with high encapsulation rate of 81.26%, and low potential of 1.46±1.71.
34688330	4	1	theme	MTT	622:624	arg1	assay					626:630	MTT assay	622:630	MTT assay	622:630	MTT assay assessed the toxicity of NPs to macrophages.
34688330	14	2	theme	macrophages	2081:2091	arg1	surface					2070:2076	the surface	2066:2076	the surface of macrophages	2066:2091	The composite NPs suppressed malignant characteristics of CC cells through down-regulation of miR-155 and activation of Tim-3 signal pathway on the surface of macrophages, promoting secretion of macrophage inflammatory factors.
34688330	11	3	theme	cell	1549:1552	arg1	accumulation					1554:1565	cell accumulation	1549:1565	cell accumulation	1549:1565	Treatment with TGF-β1-loaded chitosan NPs induced increased apoptosis rate of 9.13±2.15%, reduced migration (67.65±9.91) and invaded cells (19.98±3.41), causing cell accumulation in the S phase when compared to the blank and control groups (p < 0.05).
34688330	10	4	theme	TGF-β1	1363:1368	arg1	expression					1339:1348	positive expression	1330:1348	positive expression of CD163 and TGF-β1 (95%) (p < 0.05)	1330:1385	CC tissues had positive expression of CD163 and TGF-β1 (95%) (p < 0.05).
34688330	10	5	theme	CD163	1353:1357	arg1	expression					1339:1348	positive expression	1330:1348	positive expression of CD163 and TGF-β1 (95%) (p < 0.05)	1330:1385	CC tissues had positive expression of CD163 and TGF-β1 (95%) (p < 0.05).
34688330	2	6	theme	molecular	367:375	arg1	mechanism					377:385	the molecular mechanism	363:385	the molecular mechanism underlying chitosan NPs loaded with TGF-β1 participating in cervical cancer (CC) progression	363:478	This study aimed to explore the molecular mechanism underlying chitosan NPs loaded with TGF-β1 participating in cervical cancer (CC) progression.
34688330	12	7	theme	experimental	1649:1660	arg1	group					1662:1666	experimental group	1649:1666	experimental group	1649:1666	Besides, experimental group exhibited lower expression of miR-155 (0.39±0.59) and higher expression of Tim-3 (2.87± 0.51), which was higher than the blank group and control group.
34688330	12	8	theme	control	1805:1811	arg1	group					1813:1817	control group	1805:1817	control group	1805:1817	Besides, experimental group exhibited lower expression of miR-155 (0.39±0.59) and higher expression of Tim-3 (2.87± 0.51), which was higher than the blank group and control group.
34688330	11	9	theme	S	1574:1574	arg1	phase					1576:1580	the S phase	1570:1580	the S phase	1570:1580	Treatment with TGF-β1-loaded chitosan NPs induced increased apoptosis rate of 9.13±2.15%, reduced migration (67.65±9.91) and invaded cells (19.98±3.41), causing cell accumulation in the S phase when compared to the blank and control groups (p < 0.05).
34688330	14	10	theme	signal	2048:2053	arg1	pathway					2055:2061	Tim-3 signal pathway	2042:2061	Tim-3 signal pathway	2042:2061	The composite NPs suppressed malignant characteristics of CC cells through down-regulation of miR-155 and activation of Tim-3 signal pathway on the surface of macrophages, promoting secretion of macrophage inflammatory factors.
34688330	5	11	theme	CC	677:678	arg1	cells					680:684	CC cells	677:684	CC cells	677:684	CC cells were co-cultured with TGF-β1-loaded chitosan NPs (experimental group) or pure chitosan NPs (control group) and cells were cultured alone to produce control group.
34688330	11	12	theme	migration	1486:1494	arg1	rate					1458:1461	increased apoptosis rate	1438:1461	increased apoptosis rate of 9.13±2.15%, reduced migration (67.65±9.91) and invaded cells (19.98±3.41)	1438:1538	Treatment with TGF-β1-loaded chitosan NPs induced increased apoptosis rate of 9.13±2.15%, reduced migration (67.65±9.91) and invaded cells (19.98±3.41), causing cell accumulation in the S phase when compared to the blank and control groups (p < 0.05).
34688330	8	13	theme	low	1172:1174	arg1	potential					1176:1184	low potential	1172:1184	low potential of 1.46±1.71	1172:1197	At a ratio of 2:1 chitosan and TGF-β1, the particle size was102.65±11.98 nm, which was smallest, with high encapsulation rate of 81.26%, and low potential of 1.46±1.71.
34688330	8	14	dep	nm	1104:1105	arg1	ratio					1036:1040	a ratio	1034:1040	a ratio of 2:1 chitosan and TGF-β1	1034:1067	At a ratio of 2:1 chitosan and TGF-β1, the particle size was102.65±11.98 nm, which was smallest, with high encapsulation rate of 81.26%, and low potential of 1.46±1.71.
34688330	9	15	theme	NP	1200:1201	arg1	toxicity					1203:1210	NP toxicity	1200:1210	NP toxicity	1200:1210	NP toxicity increased as concentration rose and relative cell proliferation rate was >80%, indicated as non-toxic.
34688330	3	16	theme	NPs	601:603	arg1	distribution					540:551	particle size distribution	526:551	particle size distribution	526:551	TGF-β1-loaded-chitosan NPs were prepared and particle size distribution, zeta potential and encapsulation efficiency of NPs were determined.
34688330	3	16	theme	NPs	601:603	arg1	potential					559:567	zeta potential	554:567	zeta potential	554:567	TGF-β1-loaded-chitosan NPs were prepared and particle size distribution, zeta potential and encapsulation efficiency of NPs were determined.
34688330	3	16	theme	NPs	601:603	arg1	efficiency					587:596	encapsulation efficiency	573:596	encapsulation efficiency	573:596	TGF-β1-loaded-chitosan NPs were prepared and particle size distribution, zeta potential and encapsulation efficiency of NPs were determined.
34688330	0	17	theme	Pathway	147:153	arg1	Down-Regulation					91:105	Down-Regulation	91:105	Down-Regulation of MicroRNA-155	91:121	Chitosan Nanoparticles Loaded with TGF-β1 Inhibit Cervical Cancer Cell Progression Through Down-Regulation of MicroRNA-155 and Activation of Tim-3 Pathway.
34688330	0	17	theme	Pathway	147:153	arg1	Activation					127:136	Activation	127:136	Activation of Tim-3 Pathway	127:153	Chitosan Nanoparticles Loaded with TGF-β1 Inhibit Cervical Cancer Cell Progression Through Down-Regulation of MicroRNA-155 and Activation of Tim-3 Pathway.
34688330	5	18	theme	chitosan	722:729	arg1	NPs					731:733	TGF-β1-loaded chitosan NPs	708:733	TGF-β1-loaded chitosan NPs (experimental group)	708:754	CC cells were co-cultured with TGF-β1-loaded chitosan NPs (experimental group) or pure chitosan NPs (control group) and cells were cultured alone to produce control group.
34688330	5	18	theme	chitosan	722:729	arg1	group					749:753	experimental group	736:753	experimental group	736:753	CC cells were co-cultured with TGF-β1-loaded chitosan NPs (experimental group) or pure chitosan NPs (control group) and cells were cultured alone to produce control group.
34688330	9	19	theme	concentration	1225:1237	arg1	rose					1239:1242	concentration rose	1225:1242	concentration rose	1225:1242	NP toxicity increased as concentration rose and relative cell proliferation rate was >80%, indicated as non-toxic.
34688330	7	20	theme	Tim-3	998:1002	arg1	expression					1004:1013	miR-155 and Tim-3 expression	986:1013	miR-155 and Tim-3 expression	986:1013	Cancer cell migration was evaluated by Transwell assay, and miR-155 and Tim-3 expression was determined.
34688330	2	21	theme	cervical	447:454	arg1	CC					464:465	CC	464:465	CC	464:465	This study aimed to explore the molecular mechanism underlying chitosan NPs loaded with TGF-β1 participating in cervical cancer (CC) progression.
34688330	2	21	theme	cervical	447:454	arg1	cancer					456:461	cervical cancer	447:461	cervical cancer (CC) progression	447:478	This study aimed to explore the molecular mechanism underlying chitosan NPs loaded with TGF-β1 participating in cervical cancer (CC) progression.
34688330	11	22	theme	chitosan	1417:1424	arg1	NPs					1426:1428	TGF-β1-loaded chitosan NPs	1403:1428	TGF-β1-loaded chitosan NPs	1403:1428	Treatment with TGF-β1-loaded chitosan NPs induced increased apoptosis rate of 9.13±2.15%, reduced migration (67.65±9.91) and invaded cells (19.98±3.41), causing cell accumulation in the S phase when compared to the blank and control groups (p < 0.05).
34688330	0	23	theme	Cell	66:69	arg1	Progression					71:81	Cervical Cancer Cell Progression	50:81	Cervical Cancer Cell Progression	50:81	Chitosan Nanoparticles Loaded with TGF-β1 Inhibit Cervical Cancer Cell Progression Through Down-Regulation of MicroRNA-155 and Activation of Tim-3 Pathway.
34688330	9	24	theme	cell	1257:1260	arg1	rate					1276:1279	relative cell proliferation rate	1248:1279	relative cell proliferation rate	1248:1279	NP toxicity increased as concentration rose and relative cell proliferation rate was >80%, indicated as non-toxic.
34688330	7	25	theme	miR-155	986:992	arg1	expression					1004:1013	miR-155 and Tim-3 expression	986:1013	miR-155 and Tim-3 expression	986:1013	Cancer cell migration was evaluated by Transwell assay, and miR-155 and Tim-3 expression was determined.
34688330	1	26	theme	modified	167:174	arg1	NPs					200:202	NPs	200:202	NPs	200:202	Chemically modified chitosan nanoparticles (NPs) are capable of releasing their own substances to target cells or tissues, improving microenvironment and promoting wound healing.
34688330	1	26	theme	modified	167:174	arg1	nanoparticles					185:197	Chemically modified chitosan nanoparticles	156:197	Chemically modified chitosan nanoparticles (NPs)	156:203	Chemically modified chitosan nanoparticles (NPs) are capable of releasing their own substances to target cells or tissues, improving microenvironment and promoting wound healing.
34688330	10	27	theme	p	1377:1377	arg1	<					1379:1379	p < 0.05	1377:1384	p < 0.05	1377:1384	CC tissues had positive expression of CD163 and TGF-β1 (95%) (p < 0.05).
34688330	10	27	theme	p	1377:1377	arg1	CD163					1353:1357	CD163	1353:1357	CD163	1353:1357	CC tissues had positive expression of CD163 and TGF-β1 (95%) (p < 0.05).
34688330	13	28	theme	optimal	1824:1830	arg1	ratio					1846:1850	The optimal concentration ratio	1820:1850	The optimal concentration ratio for producing TGF-β1-loaded chitosan NPs	1820:1891	The optimal concentration ratio for producing TGF-β1-loaded chitosan NPs was 2:1, with less toxicity.
34688330	11	29	theme	apoptosis	1448:1456	arg1	rate					1458:1461	increased apoptosis rate	1438:1461	increased apoptosis rate of 9.13±2.15%, reduced migration (67.65±9.91) and invaded cells (19.98±3.41)	1438:1538	Treatment with TGF-β1-loaded chitosan NPs induced increased apoptosis rate of 9.13±2.15%, reduced migration (67.65±9.91) and invaded cells (19.98±3.41), causing cell accumulation in the S phase when compared to the blank and control groups (p < 0.05).
34688330	5	30	theme	chitosan	764:771	arg1	NPs					773:775	pure chitosan NPs	759:775	pure chitosan NPs (control group)	759:791	CC cells were co-cultured with TGF-β1-loaded chitosan NPs (experimental group) or pure chitosan NPs (control group) and cells were cultured alone to produce control group.
34688330	5	30	theme	chitosan	764:771	arg1	group					786:790	control group	778:790	control group	778:790	CC cells were co-cultured with TGF-β1-loaded chitosan NPs (experimental group) or pure chitosan NPs (control group) and cells were cultured alone to produce control group.
34688330	7	31	theme	Transwell	965:973	arg1	assay					975:979	Transwell assay	965:979	Transwell assay	965:979	Cancer cell migration was evaluated by Transwell assay, and miR-155 and Tim-3 expression was determined.
34688330	13	32	theme	less	1907:1910	arg1	toxicity					1912:1919	less toxicity	1907:1919	less toxicity	1907:1919	The optimal concentration ratio for producing TGF-β1-loaded chitosan NPs was 2:1, with less toxicity.
34688330	8	33	theme	chitosan	1049:1056	arg1	ratio					1036:1040	a ratio	1034:1040	a ratio of 2:1 chitosan and TGF-β1	1034:1067	At a ratio of 2:1 chitosan and TGF-β1, the particle size was102.65±11.98 nm, which was smallest, with high encapsulation rate of 81.26%, and low potential of 1.46±1.71.
34688330	14	34	theme	malignant	1951:1959	arg1	characteristics					1961:1975	malignant characteristics	1951:1975	malignant characteristics of CC cells	1951:1987	The composite NPs suppressed malignant characteristics of CC cells through down-regulation of miR-155 and activation of Tim-3 signal pathway on the surface of macrophages, promoting secretion of macrophage inflammatory factors.
34688330	12	35	dep	exhibited	1668:1676	arg1	higher					1773:1778	higher	1773:1778	higher	1773:1778	Besides, experimental group exhibited lower expression of miR-155 (0.39±0.59) and higher expression of Tim-3 (2.87± 0.51), which was higher than the blank group and control group.
34688330	0	36	theme	Chitosan	0:7	arg1	Nanoparticles					9:21	Chitosan Nanoparticles	0:21	Chitosan Nanoparticles Loaded with TGF-β1	0:40	Chitosan Nanoparticles Loaded with TGF-β1 Inhibit Cervical Cancer Cell Progression Through Down-Regulation of MicroRNA-155 and Activation of Tim-3 Pathway.
34688330	8	37	theme	TGF-β1	1062:1067	arg1	ratio					1036:1040	a ratio	1034:1040	a ratio of 2:1 chitosan and TGF-β1	1034:1067	At a ratio of 2:1 chitosan and TGF-β1, the particle size was102.65±11.98 nm, which was smallest, with high encapsulation rate of 81.26%, and low potential of 1.46±1.71.
34688330	13	38	theme	concentration	1832:1844	arg1	ratio					1846:1850	The optimal concentration ratio	1820:1850	The optimal concentration ratio for producing TGF-β1-loaded chitosan NPs	1820:1891	The optimal concentration ratio for producing TGF-β1-loaded chitosan NPs was 2:1, with less toxicity.
34688330	14	39	theme	inflammatory	2128:2139	arg1	factors					2141:2147	macrophage inflammatory factors	2117:2147	macrophage inflammatory factors	2117:2147	The composite NPs suppressed malignant characteristics of CC cells through down-regulation of miR-155 and activation of Tim-3 signal pathway on the surface of macrophages, promoting secretion of macrophage inflammatory factors.
34688330	3	40	theme	TGF-β1-loaded-chitosan	481:502	arg1	NPs					504:506	TGF-β1-loaded-chitosan NPs	481:506	TGF-β1-loaded-chitosan NPs	481:506	TGF-β1-loaded-chitosan NPs were prepared and particle size distribution, zeta potential and encapsulation efficiency of NPs were determined.
34688330	12	41	theme	Tim-3	1743:1747	arg1	expression					1684:1693	lower expression	1678:1693	lower expression of miR-155 (0.39±0.59)	1678:1716	Besides, experimental group exhibited lower expression of miR-155 (0.39±0.59) and higher expression of Tim-3 (2.87± 0.51), which was higher than the blank group and control group.
34688330	12	41	theme	Tim-3	1743:1747	arg1	expression					1729:1738	higher expression	1722:1738	higher expression of Tim-3 (2.87± 0.51)	1722:1760	Besides, experimental group exhibited lower expression of miR-155 (0.39±0.59) and higher expression of Tim-3 (2.87± 0.51), which was higher than the blank group and control group.
34688330	1	42	theme	wound	320:324	arg1	healing					326:332	wound healing	320:332	wound healing	320:332	Chemically modified chitosan nanoparticles (NPs) are capable of releasing their own substances to target cells or tissues, improving microenvironment and promoting wound healing.
34688330	8	43	theme	size	1083:1086	arg1	smallest					1118:1125	smallest	1118:1125	smallest	1118:1125	At a ratio of 2:1 chitosan and TGF-β1, the particle size was102.65±11.98 nm, which was smallest, with high encapsulation rate of 81.26%, and low potential of 1.46±1.71.
34688330	8	43	theme	size	1083:1086	arg1	nm					1104:1105	the particle size was102.65±11.98 nm	1070:1105	the particle size was102.65±11.98 nm	1070:1105	At a ratio of 2:1 chitosan and TGF-β1, the particle size was102.65±11.98 nm, which was smallest, with high encapsulation rate of 81.26%, and low potential of 1.46±1.71.
34688330	0	44	theme	Cervical	50:57	arg1	Cancer					59:64	Cervical Cancer	50:64	Cervical Cancer Cell Progression	50:81	Chitosan Nanoparticles Loaded with TGF-β1 Inhibit Cervical Cancer Cell Progression Through Down-Regulation of MicroRNA-155 and Activation of Tim-3 Pathway.
34688330	11	45	theme	cells	1521:1525	arg1	rate					1458:1461	increased apoptosis rate	1438:1461	increased apoptosis rate of 9.13±2.15%, reduced migration (67.65±9.91) and invaded cells (19.98±3.41)	1438:1538	Treatment with TGF-β1-loaded chitosan NPs induced increased apoptosis rate of 9.13±2.15%, reduced migration (67.65±9.91) and invaded cells (19.98±3.41), causing cell accumulation in the S phase when compared to the blank and control groups (p < 0.05).
34688330	11	46	theme	p	1629:1629	arg1	groups					1621:1626	the blank and control groups	1599:1626	groups	1621:1626	Treatment with TGF-β1-loaded chitosan NPs induced increased apoptosis rate of 9.13±2.15%, reduced migration (67.65±9.91) and invaded cells (19.98±3.41), causing cell accumulation in the S phase when compared to the blank and control groups (p < 0.05).
34688330	11	46	theme	p	1629:1629	arg1	<					1631:1631	p < 0.05	1629:1636	p < 0.05	1629:1636	Treatment with TGF-β1-loaded chitosan NPs induced increased apoptosis rate of 9.13±2.15%, reduced migration (67.65±9.91) and invaded cells (19.98±3.41), causing cell accumulation in the S phase when compared to the blank and control groups (p < 0.05).
34688330	11	47	theme	invaded	1513:1519	arg1	cells					1521:1525	invaded cells	1513:1525	invaded cells (19.98±3.41)	1513:1538	Treatment with TGF-β1-loaded chitosan NPs induced increased apoptosis rate of 9.13±2.15%, reduced migration (67.65±9.91) and invaded cells (19.98±3.41), causing cell accumulation in the S phase when compared to the blank and control groups (p < 0.05).
34688330	11	47	theme	invaded	1513:1519	arg1	19.98±3.41					1528:1537	19.98±3.41	1528:1537	19.98±3.41	1528:1537	Treatment with TGF-β1-loaded chitosan NPs induced increased apoptosis rate of 9.13±2.15%, reduced migration (67.65±9.91) and invaded cells (19.98±3.41), causing cell accumulation in the S phase when compared to the blank and control groups (p < 0.05).
34688330	7	48	theme	cell	933:936	arg1	migration					938:946	Cancer cell migration	926:946	Cancer cell migration	926:946	Cancer cell migration was evaluated by Transwell assay, and miR-155 and Tim-3 expression was determined.
34688330	1	49	theme	own	236:238	arg1	substances					240:249	their own substances	230:249	their own substances	230:249	Chemically modified chitosan nanoparticles (NPs) are capable of releasing their own substances to target cells or tissues, improving microenvironment and promoting wound healing.
34688330	10	50	theme	CC	1315:1316	arg1	tissues					1318:1324	CC tissues	1315:1324	CC tissues	1315:1324	CC tissues had positive expression of CD163 and TGF-β1 (95%) (p < 0.05).
34688330	12	51	theme	lower	1678:1682	arg1	expression					1684:1693	lower expression	1678:1693	lower expression of miR-155 (0.39±0.59)	1678:1716	Besides, experimental group exhibited lower expression of miR-155 (0.39±0.59) and higher expression of Tim-3 (2.87± 0.51), which was higher than the blank group and control group.
34688330	10	52	theme	positive	1330:1337	arg1	expression					1339:1348	positive expression	1330:1348	positive expression of CD163 and TGF-β1 (95%) (p < 0.05)	1330:1385	CC tissues had positive expression of CD163 and TGF-β1 (95%) (p < 0.05).
34688330	13	53	theme	TGF-β1-loaded	1866:1878	arg1	NPs					1889:1891	TGF-β1-loaded chitosan NPs	1866:1891	TGF-β1-loaded chitosan NPs	1866:1891	The optimal concentration ratio for producing TGF-β1-loaded chitosan NPs was 2:1, with less toxicity.
34688330	3	54	theme	size	535:538	arg1	distribution					540:551	particle size distribution	526:551	particle size distribution	526:551	TGF-β1-loaded-chitosan NPs were prepared and particle size distribution, zeta potential and encapsulation efficiency of NPs were determined.
34688330	14	55	from	down-regulation	1997:2011	arg1	surface					2070:2076	the surface	2066:2076	the surface of macrophages	2066:2091	The composite NPs suppressed malignant characteristics of CC cells through down-regulation of miR-155 and activation of Tim-3 signal pathway on the surface of macrophages, promoting secretion of macrophage inflammatory factors.
34688330	11	56	theme	reduced	1478:1484	arg1	67.65±9.91					1497:1506	67.65±9.91	1497:1506	67.65±9.91	1497:1506	Treatment with TGF-β1-loaded chitosan NPs induced increased apoptosis rate of 9.13±2.15%, reduced migration (67.65±9.91) and invaded cells (19.98±3.41), causing cell accumulation in the S phase when compared to the blank and control groups (p < 0.05).
34688330	11	56	theme	reduced	1478:1484	arg1	migration					1486:1494	reduced migration	1478:1494	reduced migration (67.65±9.91)	1478:1507	Treatment with TGF-β1-loaded chitosan NPs induced increased apoptosis rate of 9.13±2.15%, reduced migration (67.65±9.91) and invaded cells (19.98±3.41), causing cell accumulation in the S phase when compared to the blank and control groups (p < 0.05).
34688330	3	57	theme	encapsulation	573:585	arg1	efficiency					587:596	encapsulation efficiency	573:596	encapsulation efficiency	573:596	TGF-β1-loaded-chitosan NPs were prepared and particle size distribution, zeta potential and encapsulation efficiency of NPs were determined.
34688330	8	58	theme	encapsulation	1138:1150	arg1	rate					1152:1155	high encapsulation rate	1133:1155	high encapsulation rate of 81.26%	1133:1165	At a ratio of 2:1 chitosan and TGF-β1, the particle size was102.65±11.98 nm, which was smallest, with high encapsulation rate of 81.26%, and low potential of 1.46±1.71.
34688330	11	59	theme	%	1475:1475	arg1	rate					1458:1461	increased apoptosis rate	1438:1461	increased apoptosis rate of 9.13±2.15%, reduced migration (67.65±9.91) and invaded cells (19.98±3.41)	1438:1538	Treatment with TGF-β1-loaded chitosan NPs induced increased apoptosis rate of 9.13±2.15%, reduced migration (67.65±9.91) and invaded cells (19.98±3.41), causing cell accumulation in the S phase when compared to the blank and control groups (p < 0.05).
34688330	14	60	theme	pathway	2055:2061	arg1	activation					2028:2037	activation	2028:2037	activation of Tim-3 signal pathway	2028:2061	The composite NPs suppressed malignant characteristics of CC cells through down-regulation of miR-155 and activation of Tim-3 signal pathway on the surface of macrophages, promoting secretion of macrophage inflammatory factors.
34688330	14	60	theme	pathway	2055:2061	arg1	miR-155					2016:2022	miR-155	2016:2022	miR-155	2016:2022	The composite NPs suppressed malignant characteristics of CC cells through down-regulation of miR-155 and activation of Tim-3 signal pathway on the surface of macrophages, promoting secretion of macrophage inflammatory factors.
34688330	8	61	theme	%	1165:1165	arg1	rate					1152:1155	high encapsulation rate	1133:1155	high encapsulation rate of 81.26%	1133:1165	At a ratio of 2:1 chitosan and TGF-β1, the particle size was102.65±11.98 nm, which was smallest, with high encapsulation rate of 81.26%, and low potential of 1.46±1.71.
34688330	14	62	theme	Tim-3	2042:2046	arg1	pathway					2055:2061	Tim-3 signal pathway	2042:2061	Tim-3 signal pathway	2042:2061	The composite NPs suppressed malignant characteristics of CC cells through down-regulation of miR-155 and activation of Tim-3 signal pathway on the surface of macrophages, promoting secretion of macrophage inflammatory factors.
34688330	0	63	theme	Tim-3	141:145	arg1	Pathway					147:153	Tim-3 Pathway	141:153	Tim-3 Pathway	141:153	Chitosan Nanoparticles Loaded with TGF-β1 Inhibit Cervical Cancer Cell Progression Through Down-Regulation of MicroRNA-155 and Activation of Tim-3 Pathway.
34688330	14	64	theme	activation	2028:2037	arg1	down-regulation					1997:2011	down-regulation	1997:2011	down-regulation of miR-155 and activation of Tim-3 signal pathway on the surface of macrophages	1997:2091	The composite NPs suppressed malignant characteristics of CC cells through down-regulation of miR-155 and activation of Tim-3 signal pathway on the surface of macrophages, promoting secretion of macrophage inflammatory factors.
34688330	11	65	with	Treatment	1388:1396	arg1	NPs					1426:1428	TGF-β1-loaded chitosan NPs	1403:1428	TGF-β1-loaded chitosan NPs	1403:1428	Treatment with TGF-β1-loaded chitosan NPs induced increased apoptosis rate of 9.13±2.15%, reduced migration (67.65±9.91) and invaded cells (19.98±3.41), causing cell accumulation in the S phase when compared to the blank and control groups (p < 0.05).
34688330	14	66	theme	miR-155	2016:2022	arg1	down-regulation					1997:2011	down-regulation	1997:2011	down-regulation of miR-155 and activation of Tim-3 signal pathway on the surface of macrophages	1997:2091	The composite NPs suppressed malignant characteristics of CC cells through down-regulation of miR-155 and activation of Tim-3 signal pathway on the surface of macrophages, promoting secretion of macrophage inflammatory factors.
34688330	5	67	theme	pure	759:762	arg1	NPs					773:775	pure chitosan NPs	759:775	pure chitosan NPs (control group)	759:791	CC cells were co-cultured with TGF-β1-loaded chitosan NPs (experimental group) or pure chitosan NPs (control group) and cells were cultured alone to produce control group.
34688330	5	67	theme	pure	759:762	arg1	group					786:790	control group	778:790	control group	778:790	CC cells were co-cultured with TGF-β1-loaded chitosan NPs (experimental group) or pure chitosan NPs (control group) and cells were cultured alone to produce control group.
34688330	5	68	theme	TGF-β1-loaded	708:720	arg1	NPs					731:733	TGF-β1-loaded chitosan NPs	708:733	TGF-β1-loaded chitosan NPs (experimental group)	708:754	CC cells were co-cultured with TGF-β1-loaded chitosan NPs (experimental group) or pure chitosan NPs (control group) and cells were cultured alone to produce control group.
34688330	5	68	theme	TGF-β1-loaded	708:720	arg1	group					749:753	experimental group	736:753	experimental group	736:753	CC cells were co-cultured with TGF-β1-loaded chitosan NPs (experimental group) or pure chitosan NPs (control group) and cells were cultured alone to produce control group.
34688330	8	69	theme	1.46±1.71	1189:1197	arg1	smallest					1118:1125	smallest	1118:1125	smallest	1118:1125	At a ratio of 2:1 chitosan and TGF-β1, the particle size was102.65±11.98 nm, which was smallest, with high encapsulation rate of 81.26%, and low potential of 1.46±1.71.
34688330	8	69	theme	1.46±1.71	1189:1197	arg1	potential					1176:1184	low potential	1172:1184	low potential of 1.46±1.71	1172:1197	At a ratio of 2:1 chitosan and TGF-β1, the particle size was102.65±11.98 nm, which was smallest, with high encapsulation rate of 81.26%, and low potential of 1.46±1.71.
34688330	8	69	theme	1.46±1.71	1189:1197	arg1	nm					1104:1105	the particle size was102.65±11.98 nm	1070:1105	the particle size was102.65±11.98 nm	1070:1105	At a ratio of 2:1 chitosan and TGF-β1, the particle size was102.65±11.98 nm, which was smallest, with high encapsulation rate of 81.26%, and low potential of 1.46±1.71.
34688330	5	70	theme	experimental	736:747	arg1	NPs					731:733	TGF-β1-loaded chitosan NPs	708:733	TGF-β1-loaded chitosan NPs (experimental group)	708:754	CC cells were co-cultured with TGF-β1-loaded chitosan NPs (experimental group) or pure chitosan NPs (control group) and cells were cultured alone to produce control group.
34688330	5	70	theme	experimental	736:747	arg1	group					749:753	experimental group	736:753	experimental group	736:753	CC cells were co-cultured with TGF-β1-loaded chitosan NPs (experimental group) or pure chitosan NPs (control group) and cells were cultured alone to produce control group.
34688330	8	71	theme	2:1	1045:1047	arg1	chitosan					1049:1056	chitosan	1049:1056	chitosan	1049:1056	At a ratio of 2:1 chitosan and TGF-β1, the particle size was102.65±11.98 nm, which was smallest, with high encapsulation rate of 81.26%, and low potential of 1.46±1.71.
34688330	11	72	theme	TGF-β1-loaded	1403:1415	arg1	NPs					1426:1428	TGF-β1-loaded chitosan NPs	1403:1428	TGF-β1-loaded chitosan NPs	1403:1428	Treatment with TGF-β1-loaded chitosan NPs induced increased apoptosis rate of 9.13±2.15%, reduced migration (67.65±9.91) and invaded cells (19.98±3.41), causing cell accumulation in the S phase when compared to the blank and control groups (p < 0.05).
34688330	5	73	theme	control	778:784	arg1	NPs					773:775	pure chitosan NPs	759:775	pure chitosan NPs (control group)	759:791	CC cells were co-cultured with TGF-β1-loaded chitosan NPs (experimental group) or pure chitosan NPs (control group) and cells were cultured alone to produce control group.
34688330	5	73	theme	control	778:784	arg1	group					786:790	control group	778:790	control group	778:790	CC cells were co-cultured with TGF-β1-loaded chitosan NPs (experimental group) or pure chitosan NPs (control group) and cells were cultured alone to produce control group.
34688330	9	74	theme	relative	1248:1255	arg1	rate					1276:1279	relative cell proliferation rate	1248:1279	relative cell proliferation rate	1248:1279	NP toxicity increased as concentration rose and relative cell proliferation rate was >80%, indicated as non-toxic.
34688330	9	75	theme	proliferation	1262:1274	arg1	rate					1276:1279	relative cell proliferation rate	1248:1279	relative cell proliferation rate	1248:1279	NP toxicity increased as concentration rose and relative cell proliferation rate was >80%, indicated as non-toxic.
34688330	1	76	theme	chitosan	176:183	arg1	NPs					200:202	NPs	200:202	NPs	200:202	Chemically modified chitosan nanoparticles (NPs) are capable of releasing their own substances to target cells or tissues, improving microenvironment and promoting wound healing.
34688330	1	76	theme	chitosan	176:183	arg1	nanoparticles					185:197	Chemically modified chitosan nanoparticles	156:197	Chemically modified chitosan nanoparticles (NPs)	156:203	Chemically modified chitosan nanoparticles (NPs) are capable of releasing their own substances to target cells or tissues, improving microenvironment and promoting wound healing.
34688330	11	77	theme	increased	1438:1446	arg1	rate					1458:1461	increased apoptosis rate	1438:1461	increased apoptosis rate of 9.13±2.15%, reduced migration (67.65±9.91) and invaded cells (19.98±3.41)	1438:1538	Treatment with TGF-β1-loaded chitosan NPs induced increased apoptosis rate of 9.13±2.15%, reduced migration (67.65±9.91) and invaded cells (19.98±3.41), causing cell accumulation in the S phase when compared to the blank and control groups (p < 0.05).
34688330	0	78	theme	MicroRNA-155	110:121	arg1	Down-Regulation					91:105	Down-Regulation	91:105	Down-Regulation of MicroRNA-155	91:121	Chitosan Nanoparticles Loaded with TGF-β1 Inhibit Cervical Cancer Cell Progression Through Down-Regulation of MicroRNA-155 and Activation of Tim-3 Pathway.
34688330	0	78	theme	MicroRNA-155	110:121	arg1	Activation					127:136	Activation	127:136	Activation of Tim-3 Pathway	127:153	Chitosan Nanoparticles Loaded with TGF-β1 Inhibit Cervical Cancer Cell Progression Through Down-Regulation of MicroRNA-155 and Activation of Tim-3 Pathway.
34688330	12	79	theme	blank	1789:1793	arg1	group					1795:1799	the blank group	1785:1799	the blank group	1785:1799	Besides, experimental group exhibited lower expression of miR-155 (0.39±0.59) and higher expression of Tim-3 (2.87± 0.51), which was higher than the blank group and control group.
34688330	14	80	theme	CC	1980:1981	arg1	cells					1983:1987	CC cells	1980:1987	CC cells	1980:1987	The composite NPs suppressed malignant characteristics of CC cells through down-regulation of miR-155 and activation of Tim-3 signal pathway on the surface of macrophages, promoting secretion of macrophage inflammatory factors.
34688330	14	81	theme	factors	2141:2147	arg1	secretion					2104:2112	secretion	2104:2112	secretion of macrophage inflammatory factors	2104:2147	The composite NPs suppressed malignant characteristics of CC cells through down-regulation of miR-155 and activation of Tim-3 signal pathway on the surface of macrophages, promoting secretion of macrophage inflammatory factors.
34688330	2	82	theme	chitosan	398:405	arg1	NPs					407:409	chitosan NPs	398:409	chitosan NPs loaded with TGF-β1 participating in cervical cancer (CC) progression	398:478	This study aimed to explore the molecular mechanism underlying chitosan NPs loaded with TGF-β1 participating in cervical cancer (CC) progression.
34688330	5	83	theme	control	834:840	arg1	group					842:846	control group	834:846	control group	834:846	CC cells were co-cultured with TGF-β1-loaded chitosan NPs (experimental group) or pure chitosan NPs (control group) and cells were cultured alone to produce control group.
34688330	8	84	theme	particle	1074:1081	arg1	smallest					1118:1125	smallest	1118:1125	smallest	1118:1125	At a ratio of 2:1 chitosan and TGF-β1, the particle size was102.65±11.98 nm, which was smallest, with high encapsulation rate of 81.26%, and low potential of 1.46±1.71.
34688330	8	84	theme	particle	1074:1081	arg1	nm					1104:1105	the particle size was102.65±11.98 nm	1070:1105	the particle size was102.65±11.98 nm	1070:1105	At a ratio of 2:1 chitosan and TGF-β1, the particle size was102.65±11.98 nm, which was smallest, with high encapsulation rate of 81.26%, and low potential of 1.46±1.71.
34688330	2	85	theme	cancer	456:461	arg1	progression					468:478	cervical cancer (CC) progression	447:478	cervical cancer (CC) progression	447:478	This study aimed to explore the molecular mechanism underlying chitosan NPs loaded with TGF-β1 participating in cervical cancer (CC) progression.
34688330	14	86	theme	composite	1926:1934	arg1	NPs					1936:1938	The composite NPs	1922:1938	The composite NPs	1922:1938	The composite NPs suppressed malignant characteristics of CC cells through down-regulation of miR-155 and activation of Tim-3 signal pathway on the surface of macrophages, promoting secretion of macrophage inflammatory factors.
34688330	11	87	theme	blank	1603:1607	arg1	groups					1621:1626	the blank and control groups	1599:1626	groups	1621:1626	Treatment with TGF-β1-loaded chitosan NPs induced increased apoptosis rate of 9.13±2.15%, reduced migration (67.65±9.91) and invaded cells (19.98±3.41), causing cell accumulation in the S phase when compared to the blank and control groups (p < 0.05).
34688330	11	87	theme	blank	1603:1607	arg1	<					1631:1631	p < 0.05	1629:1636	p < 0.05	1629:1636	Treatment with TGF-β1-loaded chitosan NPs induced increased apoptosis rate of 9.13±2.15%, reduced migration (67.65±9.91) and invaded cells (19.98±3.41), causing cell accumulation in the S phase when compared to the blank and control groups (p < 0.05).
34688330	14	88	theme	macrophage	2117:2126	arg1	factors					2141:2147	macrophage inflammatory factors	2117:2147	macrophage inflammatory factors	2117:2147	The composite NPs suppressed malignant characteristics of CC cells through down-regulation of miR-155 and activation of Tim-3 signal pathway on the surface of macrophages, promoting secretion of macrophage inflammatory factors.
34688330	14	89	theme	cells	1983:1987	arg1	characteristics					1961:1975	malignant characteristics	1951:1975	malignant characteristics of CC cells	1951:1987	The composite NPs suppressed malignant characteristics of CC cells through down-regulation of miR-155 and activation of Tim-3 signal pathway on the surface of macrophages, promoting secretion of macrophage inflammatory factors.
34688330	13	90	theme	chitosan	1880:1887	arg1	NPs					1889:1891	TGF-β1-loaded chitosan NPs	1866:1891	TGF-β1-loaded chitosan NPs	1866:1891	The optimal concentration ratio for producing TGF-β1-loaded chitosan NPs was 2:1, with less toxicity.
34688330	0	91	theme	Cancer	59:64	arg1	Progression					71:81	Cervical Cancer Cell Progression	50:81	Cervical Cancer Cell Progression	50:81	Chitosan Nanoparticles Loaded with TGF-β1 Inhibit Cervical Cancer Cell Progression Through Down-Regulation of MicroRNA-155 and Activation of Tim-3 Pathway.
34688330	10	92	contain	had	1326:1328	arg2	expression					1339:1348	positive expression	1330:1348	positive expression of CD163 and TGF-β1 (95%) (p < 0.05)	1330:1385	CC tissues had positive expression of CD163 and TGF-β1 (95%) (p < 0.05).
34688330	10	92	contain	had	1326:1328	arg1	tissues					1318:1324	CC tissues	1315:1324	CC tissues	1315:1324	CC tissues had positive expression of CD163 and TGF-β1 (95%) (p < 0.05).
34688330	12	93	theme	higher	1722:1727	arg1	expression					1729:1738	higher expression	1722:1738	higher expression of Tim-3 (2.87± 0.51)	1722:1760	Besides, experimental group exhibited lower expression of miR-155 (0.39±0.59) and higher expression of Tim-3 (2.87± 0.51), which was higher than the blank group and control group.
34688330	6	94	theme	flow	866:869	arg1	cytometry					871:879	flow cytometry	866:879	flow cytometry	866:879	After treatment, flow cytometry was conducted to detect apoptosis and cycle.
34688330	12	95	theme	miR-155	1698:1704	arg1	expression					1684:1693	lower expression	1678:1693	lower expression of miR-155 (0.39±0.59)	1678:1716	Besides, experimental group exhibited lower expression of miR-155 (0.39±0.59) and higher expression of Tim-3 (2.87± 0.51), which was higher than the blank group and control group.
34688330	12	95	theme	miR-155	1698:1704	arg1	expression					1729:1738	higher expression	1722:1738	higher expression of Tim-3 (2.87± 0.51)	1722:1760	Besides, experimental group exhibited lower expression of miR-155 (0.39±0.59) and higher expression of Tim-3 (2.87± 0.51), which was higher than the blank group and control group.
34688330	3	96	theme	particle	526:533	arg1	distribution					540:551	particle size distribution	526:551	particle size distribution	526:551	TGF-β1-loaded-chitosan NPs were prepared and particle size distribution, zeta potential and encapsulation efficiency of NPs were determined.
34688330	7	97	theme	Cancer	926:931	arg1	migration					938:946	Cancer cell migration	926:946	Cancer cell migration	926:946	Cancer cell migration was evaluated by Transwell assay, and miR-155 and Tim-3 expression was determined.
34688330	11	98	theme	control	1613:1619	arg1	groups					1621:1626	the blank and control groups	1599:1626	groups	1621:1626	Treatment with TGF-β1-loaded chitosan NPs induced increased apoptosis rate of 9.13±2.15%, reduced migration (67.65±9.91) and invaded cells (19.98±3.41), causing cell accumulation in the S phase when compared to the blank and control groups (p < 0.05).
34688330	11	98	theme	control	1613:1619	arg1	<					1631:1631	p < 0.05	1629:1636	p < 0.05	1629:1636	Treatment with TGF-β1-loaded chitosan NPs induced increased apoptosis rate of 9.13±2.15%, reduced migration (67.65±9.91) and invaded cells (19.98±3.41), causing cell accumulation in the S phase when compared to the blank and control groups (p < 0.05).
34688330	4	99	theme	NPs	657:659	arg1	toxicity					645:652	the toxicity	641:652	the toxicity of NPs to macrophages	641:674	MTT assay assessed the toxicity of NPs to macrophages.
34688330	3	100	theme	zeta	554:557	arg1	potential					559:567	zeta potential	554:567	zeta potential	554:567	TGF-β1-loaded-chitosan NPs were prepared and particle size distribution, zeta potential and encapsulation efficiency of NPs were determined.
34688330	1	101	theme	target	254:259	arg1	cells					261:265	target cells	254:265	target cells	254:265	Chemically modified chitosan nanoparticles (NPs) are capable of releasing their own substances to target cells or tissues, improving microenvironment and promoting wound healing.
33454981	3	0	theme	dietary	1005:1011	arg1	SB					926:927	SB	926:927	SB fermented with L. casei TH14, cellulase and molasses (LCM-TMR)	926:990	The unfermented SB (SB-TMR), SB fermented with cellulase and molasses (CM-TMR), SB fermented with L. casei TH14 and molasses (LM-TMR), and SB fermented with L. casei TH14, cellulase and molasses (LCM-TMR) were used as dietary treatments.
33454981	3	0	theme	dietary	1005:1011	arg1	SB					867:868	SB	867:868	SB fermented with L. casei TH14 and molasses (LM-TMR)	867:919	The unfermented SB (SB-TMR), SB fermented with cellulase and molasses (CM-TMR), SB fermented with L. casei TH14 and molasses (LM-TMR), and SB fermented with L. casei TH14, cellulase and molasses (LCM-TMR) were used as dietary treatments.
33454981	3	0	theme	dietary	1005:1011	arg1	treatments					1013:1022	dietary treatments	1005:1022	dietary treatments	1005:1022	The unfermented SB (SB-TMR), SB fermented with cellulase and molasses (CM-TMR), SB fermented with L. casei TH14 and molasses (LM-TMR), and SB fermented with L. casei TH14, cellulase and molasses (LCM-TMR) were used as dietary treatments.
33454981	3	0	theme	dietary	1005:1011	arg1	SB					803:804	The unfermented SB	787:804	The unfermented SB (SB-TMR)	787:813	The unfermented SB (SB-TMR), SB fermented with cellulase and molasses (CM-TMR), SB fermented with L. casei TH14 and molasses (LM-TMR), and SB fermented with L. casei TH14, cellulase and molasses (LCM-TMR) were used as dietary treatments.
33454981	3	0	theme	dietary	1005:1011	arg1	SB					816:817	SB	816:817	SB fermented with cellulase and molasses (CM-TMR)	816:864	The unfermented SB (SB-TMR), SB fermented with cellulase and molasses (CM-TMR), SB fermented with L. casei TH14 and molasses (LM-TMR), and SB fermented with L. casei TH14, cellulase and molasses (LCM-TMR) were used as dietary treatments.
33454981	2	1	theme	multiparous	558:568	arg1	mid-lactation					570:582	multiparous mid-lactation	558:582	multiparous mid-lactation	558:582	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1 were assigned to a 4 × 4 Latin square design.
33454981	1	2	from	ration	413:418	arg1	effect					257:262	the effect	253:262	the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition	253:550	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	2	3	theme	Latin	766:770	arg1	design					779:784	a 4 × 4 Latin square design	758:784	a 4 × 4 Latin square design	758:784	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1 were assigned to a 4 × 4 Latin square design.
33454981	5	4	theme	cell	1695:1698	arg1	count					1700:1704	somatic cell count	1687:1704	somatic cell count	1687:1704	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	1	5	from	molasses	308:315	arg1	TMR					421:423	TMR	421:423	TMR	421:423	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	1	5	from	molasses	308:315	arg1	ration					413:418	the total mixed ration	397:418	the total mixed ration (TMR) for mid-lactation 75%	397:446	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	0	6	theme	mid-lactating	176:188	arg1	cows					208:211	mid-lactating Holstein Friesian cows	176:211	mid-lactating Holstein Friesian cows	176:211	Effect of sugarcane bagasse as industrial by-products treated with Lactobacillus casei TH14, cellulase and molasses on feed utilization, ruminal ecology and milk production of mid-lactating Holstein Friesian cows.
33454981	4	7	dep	RESULTS	1025:1031	arg1	LCM-TMR					1052:1058	LCM-TMR	1052:1058	LCM-TMR	1052:1058	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	7	dep	RESULTS	1025:1031	arg1	RESULTS					1025:1031	RESULTS CM-TMR, LM-TMR and LCM-TMR	1025:1058	RESULTS CM-TMR, LM-TMR and LCM-TMR	1025:1058	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	7	dep	RESULTS	1025:1031	arg1	LM-TMR					1041:1046	LM-TMR	1041:1046	LM-TMR	1041:1046	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	7	dep	RESULTS	1025:1031	arg1	CM-TMR					1033:1038	CM-TMR	1033:1038	CM-TMR	1033:1038	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	0	8	theme	Friesian	199:206	arg1	cows					208:211	mid-lactating Holstein Friesian cows	176:211	mid-lactating Holstein Friesian cows	176:211	Effect of sugarcane bagasse as industrial by-products treated with Lactobacillus casei TH14, cellulase and molasses on feed utilization, ruminal ecology and milk production of mid-lactating Holstein Friesian cows.
33454981	2	9	dep	crossbred	584:592	arg1	%					623:623	25%	621:623	25% Thai native breed	621:641	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1 were assigned to a 4 × 4 Latin square design.
33454981	2	9	dep	crossbred	584:592	arg1	%					597:597	75% Holstein Friesian	595:615	75% Holstein Friesian	595:615	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1 were assigned to a 4 × 4 Latin square design.
33454981	2	9	dep	crossbred	584:592	arg1	mid-lactation					570:582	multiparous mid-lactation	558:582	multiparous mid-lactation	558:582	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1 were assigned to a 4 × 4 Latin square design.
33454981	2	10	theme	crossbred	584:592	arg1	cows					650:653	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows	553:653	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1	553:739	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1 were assigned to a 4 × 4 Latin square design.
33454981	2	11	from	cows	650:653	arg1	milk					699:702	milk	699:702	milk	699:702	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1 were assigned to a 4 × 4 Latin square design.
33454981	2	12	theme	milk	716:719	arg1	yield					721:725	average milk yield 10 ± 2 kg d-1	708:739	average milk yield 10 ± 2 kg d-1	708:739	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1 were assigned to a 4 × 4 Latin square design.
33454981	5	13	theme	fatty	1562:1566	arg1	yield					1596:1600	milk yield	1591:1600	milk yield	1591:1600	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	5	13	theme	fatty	1562:1566	arg1	solids					1612:1617	total solids	1606:1617	total solids	1606:1617	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	5	13	theme	fatty	1562:1566	arg1	acids					1568:1572	volatile fatty acids	1553:1572	volatile fatty acids	1553:1572	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	5	13	theme	fatty	1562:1566	arg1	acid					1585:1588	propionic acid	1575:1588	propionic acid	1575:1588	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	0	14	from	casei	81:85	arg1	utilization					124:134	feed utilization	119:134	feed utilization	119:134	Effect of sugarcane bagasse as industrial by-products treated with Lactobacillus casei TH14, cellulase and molasses on feed utilization, ruminal ecology and milk production of mid-lactating Holstein Friesian cows.
33454981	0	14	from	casei	81:85	arg1	ecology					145:151	ruminal ecology	137:151	ruminal ecology	137:151	Effect of sugarcane bagasse as industrial by-products treated with Lactobacillus casei TH14, cellulase and molasses on feed utilization, ruminal ecology and milk production of mid-lactating Holstein Friesian cows.
33454981	0	14	from	casei	81:85	arg1	production					162:171	milk production	157:171	milk production	157:171	Effect of sugarcane bagasse as industrial by-products treated with Lactobacillus casei TH14, cellulase and molasses on feed utilization, ruminal ecology and milk production of mid-lactating Holstein Friesian cows.
33454981	2	15	from	weight	675:680	arg1	milk					699:702	milk	699:702	milk	699:702	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1 were assigned to a 4 × 4 Latin square design.
33454981	5	16	theme	SB	1421:1422	arg1	treatments					1424:1433	fermented SB treatments	1411:1433	fermented SB treatments	1411:1433	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	4	17	theme	total	1203:1207	arg1	acid					1252:1255	propionic acid	1242:1255	propionic acid	1242:1255	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	17	theme	total	1203:1207	arg1	yield					1266:1270	milk yield	1261:1270	milk yield	1261:1270	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	17	theme	total	1203:1207	arg1	P < 0.05					1231:1238	P < 0.05	1231:1238	P < 0.05	1231:1238	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	17	theme	total	1203:1207	arg1	acids					1224:1228	total volatile fatty acids	1203:1228	total volatile fatty acids (P < 0.05)	1203:1239	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	17	theme	total	1203:1207	arg1	digestibility					1116:1128	fiber digestibility	1110:1128	fiber digestibility	1110:1128	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	1	18	theme	roughage	378:385	arg1	source					387:392	an exclusive roughage source	365:392	an exclusive roughage source	365:392	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	4	19	dep	ammonia	1287:1293	arg1	P < 0.05					1356:1363	P < 0.05	1356:1363	P < 0.05	1356:1363	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	19	dep	ammonia	1287:1293	arg1	ratio					1326:1330	propionic ratio	1316:1330	propionic ratio	1316:1330	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	19	dep	ammonia	1287:1293	arg1	production					1344:1353	methane production	1336:1353	methane production	1336:1353	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	2	20	theme	dairy	644:648	arg1	cows					650:653	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows	553:653	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1	553:739	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1 were assigned to a 4 × 4 Latin square design.
33454981	4	21	theme	fatty	1218:1222	arg1	acid					1252:1255	propionic acid	1242:1255	propionic acid	1242:1255	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	21	theme	fatty	1218:1222	arg1	yield					1266:1270	milk yield	1261:1270	milk yield	1261:1270	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	21	theme	fatty	1218:1222	arg1	P < 0.05					1231:1238	P < 0.05	1231:1238	P < 0.05	1231:1238	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	21	theme	fatty	1218:1222	arg1	acids					1224:1228	total volatile fatty acids	1203:1228	total volatile fatty acids (P < 0.05)	1203:1239	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	21	theme	fatty	1218:1222	arg1	digestibility					1116:1128	fiber digestibility	1110:1128	fiber digestibility	1110:1128	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	0	22	theme	ruminal	137:143	arg1	ecology					145:151	ruminal ecology	137:151	ruminal ecology	137:151	Effect of sugarcane bagasse as industrial by-products treated with Lactobacillus casei TH14, cellulase and molasses on feed utilization, ruminal ecology and milk production of mid-lactating Holstein Friesian cows.
33454981	1	23	from	cellulase	293:301	arg1	TMR					421:423	TMR	421:423	TMR	421:423	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	1	23	from	cellulase	293:301	arg1	ration					413:418	the total mixed ration	397:418	the total mixed ration (TMR) for mid-lactation 75%	397:446	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	2	24	dep	%	623:623	arg1	breed					637:641	Thai native breed	625:641	25% Thai native breed	621:641	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1 were assigned to a 4 × 4 Latin square design.
33454981	1	25	theme	total	401:405	arg1	TMR					421:423	TMR	421:423	TMR	421:423	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	1	25	theme	total	401:405	arg1	ration					413:418	the total mixed ration	397:418	the total mixed ration (TMR) for mid-lactation 75%	397:446	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	5	26	theme	milk	1591:1594	arg1	yield					1596:1600	milk yield	1591:1600	milk yield	1591:1600	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	5	26	theme	milk	1591:1594	arg1	acids					1568:1572	volatile fatty acids	1553:1572	volatile fatty acids	1553:1572	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	2	27	theme	body	670:673	arg1	weight					675:680	439 ± 16 kg body weight	658:680	439 ± 16 kg body weight	658:680	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1 were assigned to a 4 × 4 Latin square design.
33454981	1	28	theme	combination	317:327	arg1	SB					358:359	SB	358:359	SB	358:359	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	1	28	theme	combination	317:327	arg1	bagasse					349:355	combination fermented sugarcane bagasse	317:355	molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75%	308:446	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	4	29	theme	propionic	1242:1250	arg1	acid					1252:1255	propionic acid	1242:1255	propionic acid	1242:1255	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	29	theme	propionic	1242:1250	arg1	acids					1224:1228	total volatile fatty acids	1203:1228	total volatile fatty acids (P < 0.05)	1203:1239	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	1	30	theme	sugarcane	339:347	arg1	SB					358:359	SB	358:359	SB	358:359	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	1	30	theme	sugarcane	339:347	arg1	bagasse					349:355	combination fermented sugarcane bagasse	317:355	molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75%	308:446	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	3	31	theme	L.	944:945	arg1	TH14					953:956	L. casei TH14	944:956	L. casei TH14	944:956	The unfermented SB (SB-TMR), SB fermented with cellulase and molasses (CM-TMR), SB fermented with L. casei TH14 and molasses (LM-TMR), and SB fermented with L. casei TH14, cellulase and molasses (LCM-TMR) were used as dietary treatments.
33454981	1	32	dep	molasses	308:315	arg1	SB					358:359	SB	358:359	SB	358:359	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	1	32	dep	molasses	308:315	arg1	bagasse					349:355	combination fermented sugarcane bagasse	317:355	molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75%	308:446	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	4	33	theme	dry	1095:1097	arg1	matter					1099:1104	dry matter	1095:1104	dry matter	1095:1104	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	1	34	theme	ruminal	503:509	arg1	ecology					511:517	ruminal ecology	503:517	ruminal ecology	503:517	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	2	35	theme	Thai	625:628	arg1	breed					637:641	Thai native breed	625:641	25% Thai native breed	621:641	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1 were assigned to a 4 × 4 Latin square design.
33454981	5	36	theme	blood	1493:1497	arg1	P < 0.01					1508:1515	P < 0.01	1508:1515	P < 0.01	1508:1515	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	5	36	theme	blood	1493:1497	arg1	glucose					1499:1505	greater blood glucose	1485:1505	greater blood glucose (P < 0.01)	1485:1516	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	1	37	theme	Lactobacillus	267:279	arg1	TH14					287:290	Lactobacillus casei TH14	267:290	Lactobacillus casei TH14	267:290	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	1	38	from	TH14	287:290	arg1	TMR					421:423	TMR	421:423	TMR	421:423	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	1	38	from	TH14	287:290	arg1	ration					413:418	the total mixed ration	397:418	the total mixed ration (TMR) for mid-lactation 75%	397:446	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	6	39	theme	additives	1786:1794	arg1	Combination					1753:1763	CONCLUSION Combination	1742:1763	CONCLUSION Combination of L. casei TH14 and additives (LCM-TMR)	1742:1804	CONCLUSION Combination of L. casei TH14 and additives (LCM-TMR) effectively enhanced feed use, rumen ecology and milk production of Holstein Friesian cows.
33454981	0	40	theme	sugarcane	10:18	arg1	Effect					0:5	Effect	0:5	Effect of sugarcane	0:18	Effect of sugarcane bagasse as industrial by-products treated with Lactobacillus casei TH14, cellulase and molasses on feed utilization, ruminal ecology and milk production of mid-lactating Holstein Friesian cows.
33454981	1	41	theme	TH14	287:290	arg1	effect					257:262	the effect	253:262	the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition	253:550	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	6	42	theme	Holstein	1874:1881	arg1	cows					1892:1895	Holstein Friesian cows	1874:1895	Holstein Friesian cows	1874:1895	CONCLUSION Combination of L. casei TH14 and additives (LCM-TMR) effectively enhanced feed use, rumen ecology and milk production of Holstein Friesian cows.
33454981	4	43	theme	gross	1131:1135	arg1	digestibility					1116:1128	fiber digestibility	1110:1128	fiber digestibility	1110:1128	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	43	theme	gross	1131:1135	arg1	energy					1137:1142	gross energy	1131:1142	gross energy	1131:1142	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	43	theme	gross	1131:1135	arg1	P < 0.05					1177:1184	P < 0.05	1177:1184	P < 0.05	1177:1184	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	2	44	from	215 ± 5 days	683:694	arg1	milk					699:702	milk	699:702	milk	699:702	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1 were assigned to a 4 × 4 Latin square design.
33454981	1	45	theme	milk	535:538	arg1	composition					540:550	milk composition	535:550	milk composition	535:550	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	4	46	theme	acetic	1296:1301	arg1	ammonia					1287:1293	ammonia	1287:1293	ammonia	1287:1293	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	46	theme	acetic	1296:1301	arg1	acid					1303:1306	acetic acid	1296:1306	acetic acid	1296:1306	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	1	47	theme	cellulase	293:301	arg1	effect					257:262	the effect	253:262	the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition	253:550	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	2	48	from	milk	699:702	arg1	cows					650:653	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows	553:653	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1	553:739	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1 were assigned to a 4 × 4 Latin square design.
33454981	1	49	theme	Holstein	458:465	arg1	cows					467:470	crossbred Holstein cows	448:470	crossbred Holstein cows	448:470	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	5	50	contain	had	1444:1446	arg2	glucose					1499:1505	greater blood glucose	1485:1505	greater blood glucose (P < 0.01)	1485:1516	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	5	50	contain	had	1444:1446	arg2	ammonia					1473:1479	lower (P < 0.05) ruminal ammonia	1448:1479	lower (P < 0.05) ruminal ammonia	1448:1479	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	5	50	contain	had	1444:1446	arg2	P < 0.01					1508:1515	P < 0.01	1508:1515	P < 0.01	1508:1515	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	5	50	contain	had	1444:1446	arg1	LCM-TMR					1436:1442	LCM-TMR	1436:1442	LCM-TMR	1436:1442	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	6	51	theme	L.	1768:1769	arg1	TH14					1777:1780	L. casei TH14	1768:1780	L. casei TH14	1768:1780	CONCLUSION Combination of L. casei TH14 and additives (LCM-TMR) effectively enhanced feed use, rumen ecology and milk production of Holstein Friesian cows.
33454981	7	52	theme	Chemical	1916:1923	arg1	Industry					1925:1932	Chemical Industry	1916:1932	Chemical Industry	1916:1932	© 2021 Society of Chemical Industry.
33454981	6	53	theme	rumen	1837:1841	arg1	ecology					1843:1849	rumen ecology	1837:1849	rumen ecology	1837:1849	CONCLUSION Combination of L. casei TH14 and additives (LCM-TMR) effectively enhanced feed use, rumen ecology and milk production of Holstein Friesian cows.
33454981	3	54	theme	L.	885:886	arg1	TH14					894:897	L. casei TH14	885:897	L. casei TH14	885:897	The unfermented SB (SB-TMR), SB fermented with cellulase and molasses (CM-TMR), SB fermented with L. casei TH14 and molasses (LM-TMR), and SB fermented with L. casei TH14, cellulase and molasses (LCM-TMR) were used as dietary treatments.
33454981	5	55	theme	propionic	1637:1645	arg1	P < 0.01					1654:1661	P < 0.01	1654:1661	P < 0.01	1654:1661	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	5	55	theme	propionic	1637:1645	arg1	ratio					1647:1651	propionic ratio	1637:1651	propionic ratio (P < 0.01)	1637:1662	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	2	56	from	yield	721:725	arg1	milk					699:702	milk	699:702	milk	699:702	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1 were assigned to a 4 × 4 Latin square design.
33454981	2	57	theme	square	772:777	arg1	design					779:784	a 4 × 4 Latin square design	758:784	a 4 × 4 Latin square design	758:784	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1 were assigned to a 4 × 4 Latin square design.
33454981	5	58	theme	somatic	1687:1693	arg1	count					1700:1704	somatic cell count	1687:1704	somatic cell count	1687:1704	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	4	59	theme	methane	1336:1342	arg1	production					1344:1353	methane production	1336:1353	methane production	1336:1353	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	5	60	dep	greater	1545:1551	arg1	yield					1596:1600	milk yield	1591:1600	milk yield	1591:1600	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	5	60	dep	greater	1545:1551	arg1	solids					1612:1617	total solids	1606:1617	total solids	1606:1617	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	5	60	dep	greater	1545:1551	arg1	acid					1585:1588	propionic acid	1575:1588	propionic acid	1575:1588	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	5	60	dep	greater	1545:1551	arg1	acids					1568:1572	volatile fatty acids	1553:1572	volatile fatty acids	1553:1572	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	5	60	dep	greater	1545:1551	arg1	P < 0.05					1535:1542	P < 0.05	1535:1542	P < 0.05	1535:1542	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	2	61	theme	4 × 4	760:764	arg1	design					779:784	a 4 × 4 Latin square design	758:784	a 4 × 4 Latin square design	758:784	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1 were assigned to a 4 × 4 Latin square design.
33454981	4	62	theme	blood	1188:1192	arg1	digestibility					1116:1128	fiber digestibility	1110:1128	fiber digestibility	1110:1128	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	62	theme	blood	1188:1192	arg1	glucose					1194:1200	blood glucose	1188:1200	blood glucose	1188:1200	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	0	63	theme	Holstein	190:197	arg1	cows					208:211	mid-lactating Holstein Friesian cows	176:211	mid-lactating Holstein Friesian cows	176:211	Effect of sugarcane bagasse as industrial by-products treated with Lactobacillus casei TH14, cellulase and molasses on feed utilization, ruminal ecology and milk production of mid-lactating Holstein Friesian cows.
33454981	6	64	theme	TH14	1777:1780	arg1	Combination					1753:1763	CONCLUSION Combination	1742:1763	CONCLUSION Combination of L. casei TH14 and additives (LCM-TMR)	1742:1804	CONCLUSION Combination of L. casei TH14 and additives (LCM-TMR) effectively enhanced feed use, rumen ecology and milk production of Holstein Friesian cows.
33454981	4	65	theme	energy	1162:1167	arg1	digestibility					1116:1128	fiber digestibility	1110:1128	fiber digestibility	1110:1128	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	65	theme	energy	1162:1167	arg1	intake					1169:1174	metabolizable energy intake	1148:1174	metabolizable energy intake	1148:1174	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	0	66	theme	cows	208:211	arg1	utilization					124:134	feed utilization	119:134	feed utilization	119:134	Effect of sugarcane bagasse as industrial by-products treated with Lactobacillus casei TH14, cellulase and molasses on feed utilization, ruminal ecology and milk production of mid-lactating Holstein Friesian cows.
33454981	0	66	theme	cows	208:211	arg1	ecology					145:151	ruminal ecology	137:151	ruminal ecology	137:151	Effect of sugarcane bagasse as industrial by-products treated with Lactobacillus casei TH14, cellulase and molasses on feed utilization, ruminal ecology and milk production of mid-lactating Holstein Friesian cows.
33454981	0	66	theme	cows	208:211	arg1	production					162:171	milk production	157:171	milk production	157:171	Effect of sugarcane bagasse as industrial by-products treated with Lactobacillus casei TH14, cellulase and molasses on feed utilization, ruminal ecology and milk production of mid-lactating Holstein Friesian cows.
33454981	5	67	theme	propionic	1575:1583	arg1	acids					1568:1572	volatile fatty acids	1553:1572	volatile fatty acids	1553:1572	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	5	67	theme	propionic	1575:1583	arg1	acid					1585:1588	propionic acid	1575:1588	propionic acid	1575:1588	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	6	68	theme	cows	1892:1895	arg1	use					1832:1834	feed use	1827:1834	feed use	1827:1834	CONCLUSION Combination of L. casei TH14 and additives (LCM-TMR) effectively enhanced feed use, rumen ecology and milk production of Holstein Friesian cows.
33454981	6	68	theme	cows	1892:1895	arg1	ecology					1843:1849	rumen ecology	1837:1849	rumen ecology	1837:1849	CONCLUSION Combination of L. casei TH14 and additives (LCM-TMR) effectively enhanced feed use, rumen ecology and milk production of Holstein Friesian cows.
33454981	6	68	theme	cows	1892:1895	arg1	production					1860:1869	milk production	1855:1869	milk production of Holstein Friesian cows	1855:1895	CONCLUSION Combination of L. casei TH14 and additives (LCM-TMR) effectively enhanced feed use, rumen ecology and milk production of Holstein Friesian cows.
33454981	2	69	theme	215 ± 5 days	683:694	arg1	cows					650:653	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows	553:653	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1	553:739	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1 were assigned to a 4 × 4 Latin square design.
33454981	1	70	theme	mid-lactation	430:442	arg1	%					446:446	mid-lactation 75%	430:446	mid-lactation 75%	430:446	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	2	71	theme	yield	721:725	arg1	cows					650:653	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows	553:653	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1	553:739	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1 were assigned to a 4 × 4 Latin square design.
33454981	5	72	theme	lower	1448:1452	arg1	ammonia					1473:1479	lower (P < 0.05) ruminal ammonia	1448:1479	lower (P < 0.05) ruminal ammonia	1448:1479	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	0	73	theme	feed	119:122	arg1	utilization					124:134	feed utilization	119:134	feed utilization	119:134	Effect of sugarcane bagasse as industrial by-products treated with Lactobacillus casei TH14, cellulase and molasses on feed utilization, ruminal ecology and milk production of mid-lactating Holstein Friesian cows.
33454981	5	74	theme	volatile	1553:1560	arg1	yield					1596:1600	milk yield	1591:1600	milk yield	1591:1600	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	5	74	theme	volatile	1553:1560	arg1	solids					1612:1617	total solids	1606:1617	total solids	1606:1617	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	5	74	theme	volatile	1553:1560	arg1	acids					1568:1572	volatile fatty acids	1553:1572	volatile fatty acids	1553:1572	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	5	74	theme	volatile	1553:1560	arg1	acid					1585:1588	propionic acid	1575:1588	propionic acid	1575:1588	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	2	75	theme	average	708:714	arg1	yield					721:725	average milk yield 10 ± 2 kg d-1	708:739	average milk yield 10 ± 2 kg d-1	708:739	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1 were assigned to a 4 × 4 Latin square design.
33454981	5	76	theme	fermented	1411:1419	arg1	treatments					1424:1433	fermented SB treatments	1411:1433	fermented SB treatments	1411:1433	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	1	77	theme	exclusive	368:376	arg1	source					387:392	an exclusive roughage source	365:392	an exclusive roughage source	365:392	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	5	78	theme	total	1606:1610	arg1	solids					1612:1617	total solids	1606:1617	total solids	1606:1617	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	5	78	theme	total	1606:1610	arg1	acids					1568:1572	volatile fatty acids	1553:1572	volatile fatty acids	1553:1572	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	1	79	from	effect	257:262	arg1	intake					480:485	feed intake	475:485	feed intake	475:485	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	1	79	from	effect	257:262	arg1	ration					413:418	the total mixed ration	397:418	the total mixed ration (TMR) for mid-lactation 75%	397:446	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	1	79	from	effect	257:262	arg1	digestibility					488:500	digestibility	488:500	digestibility	488:500	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	1	79	from	effect	257:262	arg1	TMR					421:423	TMR	421:423	TMR	421:423	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	1	79	from	effect	257:262	arg1	yield					525:529	milk yield	520:529	milk yield	520:529	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	1	79	from	effect	257:262	arg1	ecology					511:517	ruminal ecology	503:517	ruminal ecology	503:517	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	1	79	from	effect	257:262	arg1	composition					540:550	milk composition	535:550	milk composition	535:550	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	0	80	from	molasses	107:114	arg1	utilization					124:134	feed utilization	119:134	feed utilization	119:134	Effect of sugarcane bagasse as industrial by-products treated with Lactobacillus casei TH14, cellulase and molasses on feed utilization, ruminal ecology and milk production of mid-lactating Holstein Friesian cows.
33454981	0	80	from	molasses	107:114	arg1	ecology					145:151	ruminal ecology	137:151	ruminal ecology	137:151	Effect of sugarcane bagasse as industrial by-products treated with Lactobacillus casei TH14, cellulase and molasses on feed utilization, ruminal ecology and milk production of mid-lactating Holstein Friesian cows.
33454981	0	80	from	molasses	107:114	arg1	production					162:171	milk production	157:171	milk production	157:171	Effect of sugarcane bagasse as industrial by-products treated with Lactobacillus casei TH14, cellulase and molasses on feed utilization, ruminal ecology and milk production of mid-lactating Holstein Friesian cows.
33454981	4	81	theme	volatile	1209:1216	arg1	acid					1252:1255	propionic acid	1242:1255	propionic acid	1242:1255	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	81	theme	volatile	1209:1216	arg1	yield					1266:1270	milk yield	1261:1270	milk yield	1261:1270	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	81	theme	volatile	1209:1216	arg1	P < 0.05					1231:1238	P < 0.05	1231:1238	P < 0.05	1231:1238	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	81	theme	volatile	1209:1216	arg1	acids					1224:1228	total volatile fatty acids	1203:1228	total volatile fatty acids (P < 0.05)	1203:1239	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	81	theme	volatile	1209:1216	arg1	digestibility					1116:1128	fiber digestibility	1110:1128	fiber digestibility	1110:1128	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	0	82	theme	milk	157:160	arg1	production					162:171	milk production	157:171	milk production	157:171	Effect of sugarcane bagasse as industrial by-products treated with Lactobacillus casei TH14, cellulase and molasses on feed utilization, ruminal ecology and milk production of mid-lactating Holstein Friesian cows.
33454981	3	83	theme	unfermented	791:801	arg1	SB					926:927	SB	926:927	SB fermented with L. casei TH14, cellulase and molasses (LCM-TMR)	926:990	The unfermented SB (SB-TMR), SB fermented with cellulase and molasses (CM-TMR), SB fermented with L. casei TH14 and molasses (LM-TMR), and SB fermented with L. casei TH14, cellulase and molasses (LCM-TMR) were used as dietary treatments.
33454981	3	83	theme	unfermented	791:801	arg1	SB					816:817	SB	816:817	SB fermented with cellulase and molasses (CM-TMR)	816:864	The unfermented SB (SB-TMR), SB fermented with cellulase and molasses (CM-TMR), SB fermented with L. casei TH14 and molasses (LM-TMR), and SB fermented with L. casei TH14, cellulase and molasses (LCM-TMR) were used as dietary treatments.
33454981	3	83	theme	unfermented	791:801	arg1	treatments					1013:1022	dietary treatments	1005:1022	dietary treatments	1005:1022	The unfermented SB (SB-TMR), SB fermented with cellulase and molasses (CM-TMR), SB fermented with L. casei TH14 and molasses (LM-TMR), and SB fermented with L. casei TH14, cellulase and molasses (LCM-TMR) were used as dietary treatments.
33454981	3	83	theme	unfermented	791:801	arg1	SB					803:804	The unfermented SB	787:804	The unfermented SB (SB-TMR)	787:813	The unfermented SB (SB-TMR), SB fermented with cellulase and molasses (CM-TMR), SB fermented with L. casei TH14 and molasses (LM-TMR), and SB fermented with L. casei TH14, cellulase and molasses (LCM-TMR) were used as dietary treatments.
33454981	3	83	theme	unfermented	791:801	arg1	SB					867:868	SB	867:868	SB fermented with L. casei TH14 and molasses (LM-TMR)	867:919	The unfermented SB (SB-TMR), SB fermented with cellulase and molasses (CM-TMR), SB fermented with L. casei TH14 and molasses (LM-TMR), and SB fermented with L. casei TH14, cellulase and molasses (LCM-TMR) were used as dietary treatments.
33454981	3	83	theme	unfermented	791:801	arg1	SB-TMR					807:812	SB-TMR	807:812	SB-TMR	807:812	The unfermented SB (SB-TMR), SB fermented with cellulase and molasses (CM-TMR), SB fermented with L. casei TH14 and molasses (LM-TMR), and SB fermented with L. casei TH14, cellulase and molasses (LCM-TMR) were used as dietary treatments.
33454981	0	84	from	cellulase	93:101	arg1	utilization					124:134	feed utilization	119:134	feed utilization	119:134	Effect of sugarcane bagasse as industrial by-products treated with Lactobacillus casei TH14, cellulase and molasses on feed utilization, ruminal ecology and milk production of mid-lactating Holstein Friesian cows.
33454981	0	84	from	cellulase	93:101	arg1	ecology					145:151	ruminal ecology	137:151	ruminal ecology	137:151	Effect of sugarcane bagasse as industrial by-products treated with Lactobacillus casei TH14, cellulase and molasses on feed utilization, ruminal ecology and milk production of mid-lactating Holstein Friesian cows.
33454981	0	84	from	cellulase	93:101	arg1	production					162:171	milk production	157:171	milk production	157:171	Effect of sugarcane bagasse as industrial by-products treated with Lactobacillus casei TH14, cellulase and molasses on feed utilization, ruminal ecology and milk production of mid-lactating Holstein Friesian cows.
33454981	2	85	theme	weight	675:680	arg1	cows					650:653	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows	553:653	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1	553:739	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1 were assigned to a 4 × 4 Latin square design.
33454981	4	86	theme	milk	1261:1264	arg1	yield					1266:1270	milk yield	1261:1270	milk yield	1261:1270	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	86	theme	milk	1261:1264	arg1	acids					1224:1228	total volatile fatty acids	1203:1228	total volatile fatty acids (P < 0.05)	1203:1239	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	0	87	theme	Lactobacillus	67:79	arg1	casei					81:85	Lactobacillus casei TH14	67:90	Lactobacillus casei TH14	67:90	Effect of sugarcane bagasse as industrial by-products treated with Lactobacillus casei TH14, cellulase and molasses on feed utilization, ruminal ecology and milk production of mid-lactating Holstein Friesian cows.
33454981	1	88	theme	mixed	407:411	arg1	TMR					421:423	TMR	421:423	TMR	421:423	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	1	88	theme	mixed	407:411	arg1	ration					413:418	the total mixed ration	397:418	the total mixed ration (TMR) for mid-lactation 75%	397:446	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	1	89	dep	TH14	287:290	arg1	cows					467:470	crossbred Holstein cows	448:470	crossbred Holstein cows	448:470	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	2	90	theme	439 ± 16 kg	658:668	arg1	weight					675:680	439 ± 16 kg body weight	658:680	439 ± 16 kg body weight	658:680	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1 were assigned to a 4 × 4 Latin square design.
33454981	1	91	theme	fermented	329:337	arg1	SB					358:359	SB	358:359	SB	358:359	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	1	91	theme	fermented	329:337	arg1	bagasse					349:355	combination fermented sugarcane bagasse	317:355	molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75%	308:446	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	5	92	dep	lower	1448:1452	arg1	P < 0.05					1455:1462	P < 0.05	1455:1462	P < 0.05	1455:1462	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	2	93	dep	%	597:597	arg1	Friesian					608:615	Friesian	608:615	Friesian	608:615	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1 were assigned to a 4 × 4 Latin square design.
33454981	6	94	theme	feed	1827:1830	arg1	use					1832:1834	feed use	1827:1834	feed use	1827:1834	CONCLUSION Combination of L. casei TH14 and additives (LCM-TMR) effectively enhanced feed use, rumen ecology and milk production of Holstein Friesian cows.
33454981	2	95	theme	native	630:635	arg1	breed					637:641	Thai native breed	625:641	25% Thai native breed	621:641	Four multiparous mid-lactation crossbred (75% Holstein Friesian and 25% Thai native breed) dairy cows of 439 ± 16 kg body weight, 215 ± 5 days in milk and average milk yield 10 ± 2 kg d-1 were assigned to a 4 × 4 Latin square design.
33454981	5	96	theme	greater	1485:1491	arg1	P < 0.01					1508:1515	P < 0.01	1508:1515	P < 0.01	1508:1515	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	5	96	theme	greater	1485:1491	arg1	glucose					1499:1505	greater blood glucose	1485:1505	greater blood glucose (P < 0.01)	1485:1516	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	3	97	theme	casei	947:951	arg1	TH14					953:956	L. casei TH14	944:956	L. casei TH14	944:956	The unfermented SB (SB-TMR), SB fermented with cellulase and molasses (CM-TMR), SB fermented with L. casei TH14 and molasses (LM-TMR), and SB fermented with L. casei TH14, cellulase and molasses (LCM-TMR) were used as dietary treatments.
33454981	1	98	theme	milk	520:523	arg1	yield					525:529	milk yield	520:529	milk yield	520:529	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	1	99	theme	casei	281:285	arg1	TH14					287:290	Lactobacillus casei TH14	267:290	Lactobacillus casei TH14	267:290	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	6	100	theme	Friesian	1883:1890	arg1	cows					1892:1895	Holstein Friesian cows	1874:1895	Holstein Friesian cows	1874:1895	CONCLUSION Combination of L. casei TH14 and additives (LCM-TMR) effectively enhanced feed use, rumen ecology and milk production of Holstein Friesian cows.
33454981	0	101	theme	industrial	31:40	arg1	by-products					42:52	industrial by-products	31:52	industrial by-products treated with Lactobacillus casei TH14, cellulase and molasses on feed utilization, ruminal ecology and milk production of mid-lactating Holstein Friesian cows	31:211	Effect of sugarcane bagasse as industrial by-products treated with Lactobacillus casei TH14, cellulase and molasses on feed utilization, ruminal ecology and milk production of mid-lactating Holstein Friesian cows.
33454981	4	102	theme	metabolizable	1148:1160	arg1	digestibility					1116:1128	fiber digestibility	1110:1128	fiber digestibility	1110:1128	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	102	theme	metabolizable	1148:1160	arg1	intake					1169:1174	metabolizable energy intake	1148:1174	metabolizable energy intake	1148:1174	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	1	103	theme	crossbred	448:456	arg1	cows					467:470	crossbred Holstein cows	448:470	crossbred Holstein cows	448:470	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	6	104	theme	milk	1855:1858	arg1	production					1860:1869	milk production	1855:1869	milk production of Holstein Friesian cows	1855:1895	CONCLUSION Combination of L. casei TH14 and additives (LCM-TMR) effectively enhanced feed use, rumen ecology and milk production of Holstein Friesian cows.
33454981	3	105	used	used	997:1000	arg2	SB					803:804	The unfermented SB	787:804	The unfermented SB (SB-TMR)	787:813	The unfermented SB (SB-TMR), SB fermented with cellulase and molasses (CM-TMR), SB fermented with L. casei TH14 and molasses (LM-TMR), and SB fermented with L. casei TH14, cellulase and molasses (LCM-TMR) were used as dietary treatments.
33454981	3	105	used	used	997:1000	arg2	treatments					1013:1022	dietary treatments	1005:1022	dietary treatments	1005:1022	The unfermented SB (SB-TMR), SB fermented with cellulase and molasses (CM-TMR), SB fermented with L. casei TH14 and molasses (LM-TMR), and SB fermented with L. casei TH14, cellulase and molasses (LCM-TMR) were used as dietary treatments.
33454981	3	105	used	used	997:1000	arg2	SB-TMR					807:812	SB-TMR	807:812	SB-TMR	807:812	The unfermented SB (SB-TMR), SB fermented with cellulase and molasses (CM-TMR), SB fermented with L. casei TH14 and molasses (LM-TMR), and SB fermented with L. casei TH14, cellulase and molasses (LCM-TMR) were used as dietary treatments.
33454981	3	105	used	used	997:1000	arg2	SB					867:868	SB	867:868	SB fermented with L. casei TH14 and molasses (LM-TMR)	867:919	The unfermented SB (SB-TMR), SB fermented with cellulase and molasses (CM-TMR), SB fermented with L. casei TH14 and molasses (LM-TMR), and SB fermented with L. casei TH14, cellulase and molasses (LCM-TMR) were used as dietary treatments.
33454981	3	105	used	used	997:1000	arg2	SB					926:927	SB	926:927	SB fermented with L. casei TH14, cellulase and molasses (LCM-TMR)	926:990	The unfermented SB (SB-TMR), SB fermented with cellulase and molasses (CM-TMR), SB fermented with L. casei TH14 and molasses (LM-TMR), and SB fermented with L. casei TH14, cellulase and molasses (LCM-TMR) were used as dietary treatments.
33454981	3	105	used	used	997:1000	arg2	SB					816:817	SB	816:817	SB fermented with cellulase and molasses (CM-TMR)	816:864	The unfermented SB (SB-TMR), SB fermented with cellulase and molasses (CM-TMR), SB fermented with L. casei TH14 and molasses (LM-TMR), and SB fermented with L. casei TH14, cellulase and molasses (LCM-TMR) were used as dietary treatments.
33454981	4	106	theme	fiber	1110:1114	arg1	digestibility					1116:1128	fiber digestibility	1110:1128	fiber digestibility	1110:1128	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	106	theme	fiber	1110:1114	arg1	energy					1137:1142	gross energy	1131:1142	gross energy	1131:1142	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	106	theme	fiber	1110:1114	arg1	intake					1169:1174	metabolizable energy intake	1148:1174	metabolizable energy intake	1148:1174	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	106	theme	fiber	1110:1114	arg1	acids					1224:1228	total volatile fatty acids	1203:1228	total volatile fatty acids (P < 0.05)	1203:1239	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	106	theme	fiber	1110:1114	arg1	glucose					1194:1200	blood glucose	1188:1200	blood glucose	1188:1200	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	6	107	theme	casei	1771:1775	arg1	TH14					1777:1780	L. casei TH14	1768:1780	L. casei TH14	1768:1780	CONCLUSION Combination of L. casei TH14 and additives (LCM-TMR) effectively enhanced feed use, rumen ecology and milk production of Holstein Friesian cows.
33454981	1	108	theme	molasses	308:315	arg1	effect					257:262	the effect	253:262	the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition	253:550	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	5	109	theme	ruminal	1465:1471	arg1	ammonia					1473:1479	lower (P < 0.05) ruminal ammonia	1448:1479	lower (P < 0.05) ruminal ammonia	1448:1479	Compared with fermented SB treatments, LCM-TMR had lower (P < 0.05) ruminal ammonia and greater blood glucose (P < 0.01); LCM-TMR showed (P < 0.05) greater volatile fatty acids, propionic acid, milk yield and total solids, and lower acetic:propionic ratio (P < 0.01); methane, protozoa and somatic cell count were found to be lowest in LCM-TMR.
33454981	1	110	dep	BACKGROUND	214:223	arg1	aimed					235:239	aimed	235:239	aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition	235:550	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	1	111	theme	feed	475:478	arg1	intake					480:485	feed intake	475:485	feed intake	475:485	BACKGROUND The study aimed to evaluate the effect of Lactobacillus casei TH14, cellulase, and molasses combination fermented sugarcane bagasse (SB) as an exclusive roughage source in the total mixed ration (TMR) for mid-lactation 75% crossbred Holstein cows on feed intake, digestibility, ruminal ecology, milk yield and milk composition.
33454981	4	112	dep	significantly	1060:1072	arg1	P < 0.01					1075:1082	P < 0.01	1075:1082	P < 0.01	1075:1082	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	3	113	theme	casei	888:892	arg1	TH14					894:897	L. casei TH14	885:897	L. casei TH14	885:897	The unfermented SB (SB-TMR), SB fermented with cellulase and molasses (CM-TMR), SB fermented with L. casei TH14 and molasses (LM-TMR), and SB fermented with L. casei TH14, cellulase and molasses (LCM-TMR) were used as dietary treatments.
33454981	6	114	theme	CONCLUSION	1742:1751	arg1	Combination					1753:1763	CONCLUSION Combination	1742:1763	CONCLUSION Combination of L. casei TH14 and additives (LCM-TMR)	1742:1804	CONCLUSION Combination of L. casei TH14 and additives (LCM-TMR) effectively enhanced feed use, rumen ecology and milk production of Holstein Friesian cows.
33454981	4	115	theme	propionic	1316:1324	arg1	P < 0.05					1356:1363	P < 0.05	1356:1363	P < 0.05	1356:1363	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33454981	4	115	theme	propionic	1316:1324	arg1	ratio					1326:1330	propionic ratio	1316:1330	propionic ratio	1316:1330	RESULTS CM-TMR, LM-TMR and LCM-TMR significantly (P < 0.01) increased dry matter and fiber digestibility, gross energy and metabolizable energy intake (P < 0.05), blood glucose, total volatile fatty acids (P < 0.05), propionic acid and milk yield, but decreased ammonia, acetic acid, acetic:propionic ratio and methane production (P < 0.05) when compared with the SB-TMR.
33963244	7	0	theme	triterpenoid	1107:1118	arg1	biosynthesis					1128:1139	triterpenoid saponin biosynthesis	1107:1139	triterpenoid saponin biosynthesis between calli	1107:1153	Associating platycodin D and platycoside E content with the expression level of genes involved in triterpenoid saponin biosynthesis between calli and leaves, three contigs were screened as putative sequences of β-Glucosidase gene converting platycoside E to platycodin D. Besides, we inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus.
33963244	5	1	theme	total	833:837	arg1	content					854:860	total polysaccharide content	833:860	total polysaccharide content	833:860	Platycodin D and total polysaccharide content of calli was found higher than that of leaves.
33963244	9	2	from	biosynthesis	1743:1754	arg1	P. grandiflorus					1759:1773	P. grandiflorus	1759:1773	P. grandiflorus	1759:1773	This study will help uncover the molecular mechanism of triterpenoid saponins biosynthesis in P. grandiflorus.
33963244	3	3	theme	experimental	685:696	arg1	use					698:700	experimental use	685:700	experimental use	685:700	An L9(34) orthogonal experiment was performed to establish a protocol for calli induction as follows: the optimal explant is stems with nodes and the optimum medium formula is MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L to obtain callus for experimental use.
33963244	7	4	theme	saponin	1120:1126	arg1	biosynthesis					1128:1139	triterpenoid saponin biosynthesis	1107:1139	triterpenoid saponin biosynthesis between calli	1107:1153	Associating platycodin D and platycoside E content with the expression level of genes involved in triterpenoid saponin biosynthesis between calli and leaves, three contigs were screened as putative sequences of β-Glucosidase gene converting platycoside E to platycodin D. Besides, we inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus.
33963244	2	5	theme	platycodin	366:375	arg1	D					377:377	platycodin D	366:377	platycodin D	366:377	Studies have shown β-Glucosidase from bacteria can convert platycoside E to platycodin D, indicating the potential existence of similar enzymes in P. grandiflorus.
33963244	1	6	theme	glycosyl	260:267	arg1	groups					269:274	two glycosyl groups	256:274	two glycosyl groups	256:274	Platycodin D and platycoside E are two triterpenoid saponins in Platycodon grandiflorus, differing only by two glycosyl groups structurally.
33963244	3	7	with	stems	579:583	arg1	nodes					590:594	nodes	590:594	nodes	590:594	An L9(34) orthogonal experiment was performed to establish a protocol for calli induction as follows: the optimal explant is stems with nodes and the optimum medium formula is MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L to obtain callus for experimental use.
33963244	7	8	theme	key	1365:1367	arg1	enzymes					1369:1375	key enzymes	1365:1375	key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus	1365:1470	Associating platycodin D and platycoside E content with the expression level of genes involved in triterpenoid saponin biosynthesis between calli and leaves, three contigs were screened as putative sequences of β-Glucosidase gene converting platycoside E to platycodin D. Besides, we inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus.
33963244	0	9	dep	transcriptome	104:116	arg1	analysis					139:146	analysis	139:146	analysis	139:146	A candidate gene identified in converting platycoside E to platycodin D from Platycodon grandiflorus by transcriptome and main metabolites analysis.
33963244	4	10	theme	total	739:743	arg1	polysaccharides					745:759	total polysaccharides	739:759	total polysaccharides	739:759	The platycodin D, platycoside E and total polysaccharides content between callus and plant organs varied wildly.
33963244	7	11	theme	putative	1198:1205	arg1	sequences					1207:1215	putative sequences	1198:1215	putative sequences of β-Glucosidase gene converting platycoside E to platycodin D. Besides, we inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus	1198:1470	Associating platycodin D and platycoside E content with the expression level of genes involved in triterpenoid saponin biosynthesis between calli and leaves, three contigs were screened as putative sequences of β-Glucosidase gene converting platycoside E to platycodin D. Besides, we inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus.
33963244	7	11	theme	putative	1198:1205	arg1	contigs					1173:1179	three contigs	1167:1179	three contigs	1167:1179	Associating platycodin D and platycoside E content with the expression level of genes involved in triterpenoid saponin biosynthesis between calli and leaves, three contigs were screened as putative sequences of β-Glucosidase gene converting platycoside E to platycodin D. Besides, we inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus.
33963244	7	12	theme	grandiflorus	1459:1470	arg1	pathway					1445:1451	triterpernoid saponins and polysaccharides biosynthesis pathway	1389:1451	triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus	1389:1470	Associating platycodin D and platycoside E content with the expression level of genes involved in triterpenoid saponin biosynthesis between calli and leaves, three contigs were screened as putative sequences of β-Glucosidase gene converting platycoside E to platycodin D. Besides, we inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus.
33963244	7	13	theme	platycoside	1250:1260	arg1	E					1262:1262	platycoside E	1250:1262	platycoside E	1250:1262	Associating platycodin D and platycoside E content with the expression level of genes involved in triterpenoid saponin biosynthesis between calli and leaves, three contigs were screened as putative sequences of β-Glucosidase gene converting platycoside E to platycodin D. Besides, we inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus.
33963244	4	14	theme	platycoside	721:731	arg1	E					733:733	platycoside E	721:733	platycoside E	721:733	The platycodin D, platycoside E and total polysaccharides content between callus and plant organs varied wildly.
33963244	5	15	theme	calli	865:869	arg1	content					854:860	total polysaccharide content	833:860	total polysaccharide content	833:860	Platycodin D and total polysaccharide content of calli was found higher than that of leaves.
33963244	5	15	theme	calli	865:869	arg1	D					827:827	Platycodin D	816:827	Platycodin D	816:827	Platycodin D and total polysaccharide content of calli was found higher than that of leaves.
33963244	8	16	from	synthesis	1615:1623	arg1	grandiflorus					1631:1642	P. grandiflorus	1628:1642	P. grandiflorus	1628:1642	Totally, a candidate gene encoding enzyme involved in converting platycoside E to platycodin D, and putative genes involved in polysaccharide synthesis in P. grandiflorus had been identified.
33963244	7	17	theme	transcription	1312:1324	arg1	factors					1326:1332	some transcription factors	1307:1332	some transcription factors	1307:1332	Associating platycodin D and platycoside E content with the expression level of genes involved in triterpenoid saponin biosynthesis between calli and leaves, three contigs were screened as putative sequences of β-Glucosidase gene converting platycoside E to platycodin D. Besides, we inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus.
33963244	1	18	theme	platycoside	166:176	arg1	saponins					201:208	two triterpenoid saponins	184:208	two triterpenoid saponins in Platycodon grandiflorus	184:235	Platycodin D and platycoside E are two triterpenoid saponins in Platycodon grandiflorus, differing only by two glycosyl groups structurally.
33963244	1	18	theme	platycoside	166:176	arg1	D					160:160	Platycodin D	149:160	Platycodin D	149:160	Platycodin D and platycoside E are two triterpenoid saponins in Platycodon grandiflorus, differing only by two glycosyl groups structurally.
33963244	1	18	theme	platycoside	166:176	arg1	E					178:178	platycoside E	166:178	platycoside E	166:178	Platycodin D and platycoside E are two triterpenoid saponins in Platycodon grandiflorus, differing only by two glycosyl groups structurally.
33963244	7	19	dep	genes	1089:1093	arg1	involved					1095:1102	involved	1095:1102	involved in triterpenoid saponin biosynthesis between calli	1095:1153	Associating platycodin D and platycoside E content with the expression level of genes involved in triterpenoid saponin biosynthesis between calli and leaves, three contigs were screened as putative sequences of β-Glucosidase gene converting platycoside E to platycodin D. Besides, we inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus.
33963244	7	19	dep	genes	1089:1093	arg1	leaves					1159:1164	leaves	1159:1164	leaves	1159:1164	Associating platycodin D and platycoside E content with the expression level of genes involved in triterpenoid saponin biosynthesis between calli and leaves, three contigs were screened as putative sequences of β-Glucosidase gene converting platycoside E to platycodin D. Besides, we inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus.
33963244	7	20	theme	genes	1089:1093	arg1	level					1080:1084	the expression level	1065:1084	the expression level of genes involved in triterpenoid saponin biosynthesis between calli and leaves	1065:1164	Associating platycodin D and platycoside E content with the expression level of genes involved in triterpenoid saponin biosynthesis between calli and leaves, three contigs were screened as putative sequences of β-Glucosidase gene converting platycoside E to platycodin D. Besides, we inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus.
33963244	0	21	dep	Platycodon	77:86	arg1	grandiflorus					88:99	Platycodon grandiflorus	77:99	Platycodon grandiflorus	77:99	A candidate gene identified in converting platycoside E to platycodin D from Platycodon grandiflorus by transcriptome and main metabolites analysis.
33963244	7	22	dep	D.	1278:1279	arg1	inferred					1293:1300	inferred	1293:1300	inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus	1293:1470	Associating platycodin D and platycoside E content with the expression level of genes involved in triterpenoid saponin biosynthesis between calli and leaves, three contigs were screened as putative sequences of β-Glucosidase gene converting platycoside E to platycodin D. Besides, we inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus.
33963244	0	23	theme	candidate	2:10	arg1	gene					12:15	A candidate gene	0:15	A candidate gene	0:15	A candidate gene identified in converting platycoside E to platycodin D from Platycodon grandiflorus by transcriptome and main metabolites analysis.
33963244	8	24	theme	platycodin	1555:1564	arg1	D					1566:1566	platycodin D	1555:1566	platycodin D	1555:1566	Totally, a candidate gene encoding enzyme involved in converting platycoside E to platycodin D, and putative genes involved in polysaccharide synthesis in P. grandiflorus had been identified.
33963244	7	25	theme	expression	1069:1078	arg1	level					1080:1084	the expression level	1065:1084	the expression level of genes involved in triterpenoid saponin biosynthesis between calli and leaves	1065:1164	Associating platycodin D and platycoside E content with the expression level of genes involved in triterpenoid saponin biosynthesis between calli and leaves, three contigs were screened as putative sequences of β-Glucosidase gene converting platycoside E to platycodin D. Besides, we inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus.
33963244	7	26	theme	β-Glucosidase	1220:1232	arg1	gene					1234:1237	β-Glucosidase gene	1220:1237	β-Glucosidase gene converting platycoside E to platycodin D. Besides, we inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus	1220:1470	Associating platycodin D and platycoside E content with the expression level of genes involved in triterpenoid saponin biosynthesis between calli and leaves, three contigs were screened as putative sequences of β-Glucosidase gene converting platycoside E to platycodin D. Besides, we inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus.
33963244	7	27	theme	polysaccharides	1416:1430	arg1	pathway					1445:1451	triterpernoid saponins and polysaccharides biosynthesis pathway	1389:1451	triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus	1389:1470	Associating platycodin D and platycoside E content with the expression level of genes involved in triterpenoid saponin biosynthesis between calli and leaves, three contigs were screened as putative sequences of β-Glucosidase gene converting platycoside E to platycodin D. Besides, we inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus.
33963244	2	28	theme	potential	395:403	arg1	existence					405:413	the potential existence	391:413	the potential existence of similar enzymes in P. grandiflorus	391:451	Studies have shown β-Glucosidase from bacteria can convert platycoside E to platycodin D, indicating the potential existence of similar enzymes in P. grandiflorus.
33963244	5	29	theme	Platycodin	816:825	arg1	D					827:827	Platycodin D	816:827	Platycodin D	816:827	Platycodin D and total polysaccharide content of calli was found higher than that of leaves.
33963244	9	30	from	mechanism	1708:1716	arg1	P. grandiflorus					1759:1773	P. grandiflorus	1759:1773	P. grandiflorus	1759:1773	This study will help uncover the molecular mechanism of triterpenoid saponins biosynthesis in P. grandiflorus.
33963244	4	31	theme	E	733:733	arg1	content					761:767	The platycodin D, platycoside E and total polysaccharides content	703:767	The platycodin D, platycoside E and total polysaccharides content between callus and plant organs	703:799	The platycodin D, platycoside E and total polysaccharides content between callus and plant organs varied wildly.
33963244	6	32	theme	calli	966:970	arg1	E					928:928	platycoside E	916:928	platycoside E	916:928	While, platycoside E and total polysaccharide content of calli was found lower than that of leaves.
33963244	6	32	theme	calli	966:970	arg1	content					955:961	total polysaccharide content	934:961	total polysaccharide content	934:961	While, platycoside E and total polysaccharide content of calli was found lower than that of leaves.
33963244	0	33	theme	main	122:125	arg1	metabolites					127:137	main metabolites	122:137	main metabolites	122:137	A candidate gene identified in converting platycoside E to platycodin D from Platycodon grandiflorus by transcriptome and main metabolites analysis.
33963244	4	34	theme	polysaccharides	745:759	arg1	content					761:767	The platycodin D, platycoside E and total polysaccharides content	703:767	The platycodin D, platycoside E and total polysaccharides content between callus and plant organs	703:799	The platycodin D, platycoside E and total polysaccharides content between callus and plant organs varied wildly.
33963244	1	35	theme	triterpenoid	188:199	arg1	saponins					201:208	two triterpenoid saponins	184:208	two triterpenoid saponins in Platycodon grandiflorus	184:235	Platycodin D and platycoside E are two triterpenoid saponins in Platycodon grandiflorus, differing only by two glycosyl groups structurally.
33963244	1	35	theme	triterpenoid	188:199	arg1	D					160:160	Platycodin D	149:160	Platycodin D	149:160	Platycodin D and platycoside E are two triterpenoid saponins in Platycodon grandiflorus, differing only by two glycosyl groups structurally.
33963244	1	35	theme	triterpenoid	188:199	arg1	E					178:178	platycoside E	166:178	platycoside E	166:178	Platycodin D and platycoside E are two triterpenoid saponins in Platycodon grandiflorus, differing only by two glycosyl groups structurally.
33963244	7	36	theme	biosynthesis	1432:1443	arg1	pathway					1445:1451	triterpernoid saponins and polysaccharides biosynthesis pathway	1389:1451	triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus	1389:1470	Associating platycodin D and platycoside E content with the expression level of genes involved in triterpenoid saponin biosynthesis between calli and leaves, three contigs were screened as putative sequences of β-Glucosidase gene converting platycoside E to platycodin D. Besides, we inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus.
33963244	9	37	theme	saponins	1734:1741	arg1	biosynthesis					1743:1754	triterpenoid saponins biosynthesis	1721:1754	triterpenoid saponins biosynthesis in P. grandiflorus	1721:1773	This study will help uncover the molecular mechanism of triterpenoid saponins biosynthesis in P. grandiflorus.
33963244	3	38	theme	1.0 mg/L + 6-BA	639:653	arg1	0.5 mg/L					655:662	MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L	630:662	MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L to obtain callus for experimental use	630:700	An L9(34) orthogonal experiment was performed to establish a protocol for calli induction as follows: the optimal explant is stems with nodes and the optimum medium formula is MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L to obtain callus for experimental use.
33963244	3	38	theme	1.0 mg/L + 6-BA	639:653	arg1	formula					619:625	the optimum medium formula	600:625	the optimum medium formula	600:625	An L9(34) orthogonal experiment was performed to establish a protocol for calli induction as follows: the optimal explant is stems with nodes and the optimum medium formula is MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L to obtain callus for experimental use.
33963244	3	39	theme	optimal	560:566	arg1	stems					579:583	stems	579:583	stems with nodes	579:594	An L9(34) orthogonal experiment was performed to establish a protocol for calli induction as follows: the optimal explant is stems with nodes and the optimum medium formula is MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L to obtain callus for experimental use.
33963244	3	39	theme	optimal	560:566	arg1	explant					568:574	the optimal explant	556:574	the optimal explant	556:574	An L9(34) orthogonal experiment was performed to establish a protocol for calli induction as follows: the optimal explant is stems with nodes and the optimum medium formula is MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L to obtain callus for experimental use.
33963244	5	40	theme	polysaccharide	839:852	arg1	content					854:860	total polysaccharide content	833:860	total polysaccharide content	833:860	Platycodin D and total polysaccharide content of calli was found higher than that of leaves.
33963244	8	41	theme	putative	1573:1580	arg1	genes					1582:1586	putative genes	1573:1586	putative genes involved in polysaccharide synthesis in P. grandiflorus	1573:1642	Totally, a candidate gene encoding enzyme involved in converting platycoside E to platycodin D, and putative genes involved in polysaccharide synthesis in P. grandiflorus had been identified.
33963244	7	42	theme	E	1050:1050	arg1	content					1052:1058	platycoside E content	1038:1058	platycoside E content	1038:1058	Associating platycodin D and platycoside E content with the expression level of genes involved in triterpenoid saponin biosynthesis between calli and leaves, three contigs were screened as putative sequences of β-Glucosidase gene converting platycoside E to platycodin D. Besides, we inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus.
33963244	9	43	theme	molecular	1698:1706	arg1	mechanism					1708:1716	the molecular mechanism	1694:1716	the molecular mechanism of triterpenoid saponins biosynthesis in P. grandiflorus	1694:1773	This study will help uncover the molecular mechanism of triterpenoid saponins biosynthesis in P. grandiflorus.
33963244	3	44	with	0.5 mg/L	655:662	arg1	nodes					590:594	nodes	590:594	nodes	590:594	An L9(34) orthogonal experiment was performed to establish a protocol for calli induction as follows: the optimal explant is stems with nodes and the optimum medium formula is MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L to obtain callus for experimental use.
33963244	2	45	theme	enzymes	426:432	arg1	existence					405:413	the potential existence	391:413	the potential existence of similar enzymes in P. grandiflorus	391:451	Studies have shown β-Glucosidase from bacteria can convert platycoside E to platycodin D, indicating the potential existence of similar enzymes in P. grandiflorus.
33963244	3	46	theme	L9	457:458	arg1	experiment					475:484	An L9(34) orthogonal experiment	454:484	An L9(34) orthogonal experiment	454:484	An L9(34) orthogonal experiment was performed to establish a protocol for calli induction as follows: the optimal explant is stems with nodes and the optimum medium formula is MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L to obtain callus for experimental use.
33963244	6	47	theme	polysaccharide	940:953	arg1	content					955:961	total polysaccharide content	934:961	total polysaccharide content	934:961	While, platycoside E and total polysaccharide content of calli was found lower than that of leaves.
33963244	7	48	theme	triterpernoid	1389:1401	arg1	saponins					1403:1410	triterpernoid saponins	1389:1410	triterpernoid saponins	1389:1410	Associating platycodin D and platycoside E content with the expression level of genes involved in triterpenoid saponin biosynthesis between calli and leaves, three contigs were screened as putative sequences of β-Glucosidase gene converting platycoside E to platycodin D. Besides, we inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus.
33963244	2	49	theme	similar	418:424	arg1	enzymes					426:432	similar enzymes	418:432	similar enzymes in P. grandiflorus	418:451	Studies have shown β-Glucosidase from bacteria can convert platycoside E to platycodin D, indicating the potential existence of similar enzymes in P. grandiflorus.
33963244	6	50	theme	total	934:938	arg1	content					955:961	total polysaccharide content	934:961	total polysaccharide content	934:961	While, platycoside E and total polysaccharide content of calli was found lower than that of leaves.
33963244	3	51	theme	MS + NAA	630:637	arg1	0.5 mg/L					655:662	MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L	630:662	MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L to obtain callus for experimental use	630:700	An L9(34) orthogonal experiment was performed to establish a protocol for calli induction as follows: the optimal explant is stems with nodes and the optimum medium formula is MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L to obtain callus for experimental use.
33963244	3	51	theme	MS + NAA	630:637	arg1	formula					619:625	the optimum medium formula	600:625	the optimum medium formula	600:625	An L9(34) orthogonal experiment was performed to establish a protocol for calli induction as follows: the optimal explant is stems with nodes and the optimum medium formula is MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L to obtain callus for experimental use.
33963244	7	52	theme	enzymes	1369:1375	arg1	expression					1351:1360	the expression	1347:1360	the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus	1347:1470	Associating platycodin D and platycoside E content with the expression level of genes involved in triterpenoid saponin biosynthesis between calli and leaves, three contigs were screened as putative sequences of β-Glucosidase gene converting platycoside E to platycodin D. Besides, we inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus.
33963244	4	53	theme	D	718:718	arg1	content					761:767	The platycodin D, platycoside E and total polysaccharides content	703:767	The platycodin D, platycoside E and total polysaccharides content between callus and plant organs	703:799	The platycodin D, platycoside E and total polysaccharides content between callus and plant organs varied wildly.
33963244	0	54	theme	platycoside	42:52	arg1	E					54:54	platycoside E	42:54	platycoside E to platycodin D from Platycodon grandiflorus	42:99	A candidate gene identified in converting platycoside E to platycodin D from Platycodon grandiflorus by transcriptome and main metabolites analysis.
33963244	7	55	theme	platycoside	1038:1048	arg1	content					1052:1058	platycoside E content	1038:1058	platycoside E content	1038:1058	Associating platycodin D and platycoside E content with the expression level of genes involved in triterpenoid saponin biosynthesis between calli and leaves, three contigs were screened as putative sequences of β-Glucosidase gene converting platycoside E to platycodin D. Besides, we inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus.
33963244	9	56	theme	biosynthesis	1743:1754	arg1	mechanism					1708:1716	the molecular mechanism	1694:1716	the molecular mechanism of triterpenoid saponins biosynthesis in P. grandiflorus	1694:1773	This study will help uncover the molecular mechanism of triterpenoid saponins biosynthesis in P. grandiflorus.
33963244	4	57	theme	platycodin	707:716	arg1	D					718:718	platycodin D	707:718	platycodin D	707:718	The platycodin D, platycoside E and total polysaccharides content between callus and plant organs varied wildly.
33963244	8	58	theme	polysaccharide	1600:1613	arg1	synthesis					1615:1623	polysaccharide synthesis	1600:1623	polysaccharide synthesis in P. grandiflorus	1600:1642	Totally, a candidate gene encoding enzyme involved in converting platycoside E to platycodin D, and putative genes involved in polysaccharide synthesis in P. grandiflorus had been identified.
33963244	7	59	theme	platycodin	1021:1030	arg1	D					1032:1032	platycodin D	1021:1032	platycodin D	1021:1032	Associating platycodin D and platycoside E content with the expression level of genes involved in triterpenoid saponin biosynthesis between calli and leaves, three contigs were screened as putative sequences of β-Glucosidase gene converting platycoside E to platycodin D. Besides, we inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus.
33963244	8	60	theme	candidate	1484:1492	arg1	gene					1494:1497	a candidate gene	1482:1497	a candidate gene encoding enzyme involved in converting platycoside E to platycodin D	1482:1566	Totally, a candidate gene encoding enzyme involved in converting platycoside E to platycodin D, and putative genes involved in polysaccharide synthesis in P. grandiflorus had been identified.
33963244	3	61	theme	medium	612:617	arg1	0.5 mg/L					655:662	MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L	630:662	MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L to obtain callus for experimental use	630:700	An L9(34) orthogonal experiment was performed to establish a protocol for calli induction as follows: the optimal explant is stems with nodes and the optimum medium formula is MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L to obtain callus for experimental use.
33963244	3	61	theme	medium	612:617	arg1	formula					619:625	the optimum medium formula	600:625	the optimum medium formula	600:625	An L9(34) orthogonal experiment was performed to establish a protocol for calli induction as follows: the optimal explant is stems with nodes and the optimum medium formula is MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L to obtain callus for experimental use.
33963244	1	62	from	saponins	201:208	arg1	Platycodon grandiflorus					213:235	Platycodon grandiflorus	213:235	Platycodon grandiflorus	213:235	Platycodin D and platycoside E are two triterpenoid saponins in Platycodon grandiflorus, differing only by two glycosyl groups structurally.
33963244	2	63	theme	platycoside	349:359	arg1	E					361:361	platycoside E	349:361	platycoside E	349:361	Studies have shown β-Glucosidase from bacteria can convert platycoside E to platycodin D, indicating the potential existence of similar enzymes in P. grandiflorus.
33963244	7	64	theme	saponins	1403:1410	arg1	pathway					1445:1451	triterpernoid saponins and polysaccharides biosynthesis pathway	1389:1451	triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus	1389:1470	Associating platycodin D and platycoside E content with the expression level of genes involved in triterpenoid saponin biosynthesis between calli and leaves, three contigs were screened as putative sequences of β-Glucosidase gene converting platycoside E to platycodin D. Besides, we inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus.
33963244	2	65	from	existence	405:413	arg1	P. grandiflorus					437:451	P. grandiflorus	437:451	P. grandiflorus	437:451	Studies have shown β-Glucosidase from bacteria can convert platycoside E to platycodin D, indicating the potential existence of similar enzymes in P. grandiflorus.
33963244	4	66	theme	plant	788:792	arg1	organs					794:799	plant organs	788:799	plant organs	788:799	The platycodin D, platycoside E and total polysaccharides content between callus and plant organs varied wildly.
33963244	2	67	from	enzymes	426:432	arg1	P. grandiflorus					437:451	P. grandiflorus	437:451	P. grandiflorus	437:451	Studies have shown β-Glucosidase from bacteria can convert platycoside E to platycodin D, indicating the potential existence of similar enzymes in P. grandiflorus.
33963244	3	68	theme	calli	528:532	arg1	induction					534:542	calli induction	528:542	calli induction	528:542	An L9(34) orthogonal experiment was performed to establish a protocol for calli induction as follows: the optimal explant is stems with nodes and the optimum medium formula is MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L to obtain callus for experimental use.
33963244	3	69	dep	follows	547:553	arg1	stems					579:583	stems	579:583	stems with nodes	579:594	An L9(34) orthogonal experiment was performed to establish a protocol for calli induction as follows: the optimal explant is stems with nodes and the optimum medium formula is MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L to obtain callus for experimental use.
33963244	3	69	dep	follows	547:553	arg1	0.5 mg/L					655:662	MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L	630:662	MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L to obtain callus for experimental use	630:700	An L9(34) orthogonal experiment was performed to establish a protocol for calli induction as follows: the optimal explant is stems with nodes and the optimum medium formula is MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L to obtain callus for experimental use.
33963244	3	69	dep	follows	547:553	arg1	explant					568:574	the optimal explant	556:574	the optimal explant	556:574	An L9(34) orthogonal experiment was performed to establish a protocol for calli induction as follows: the optimal explant is stems with nodes and the optimum medium formula is MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L to obtain callus for experimental use.
33963244	3	69	dep	follows	547:553	arg1	formula					619:625	the optimum medium formula	600:625	the optimum medium formula	600:625	An L9(34) orthogonal experiment was performed to establish a protocol for calli induction as follows: the optimal explant is stems with nodes and the optimum medium formula is MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L to obtain callus for experimental use.
33963244	0	70	theme	platycodin	59:68	arg1	D					70:70	platycodin D	59:70	platycodin D	59:70	A candidate gene identified in converting platycoside E to platycodin D from Platycodon grandiflorus by transcriptome and main metabolites analysis.
33963244	0	71	from	Platycodon	77:86	arg1	E					54:54	platycoside E	42:54	platycoside E to platycodin D from Platycodon grandiflorus	42:99	A candidate gene identified in converting platycoside E to platycodin D from Platycodon grandiflorus by transcriptome and main metabolites analysis.
33963244	3	72	theme	orthogonal	464:473	arg1	experiment					475:484	An L9(34) orthogonal experiment	454:484	An L9(34) orthogonal experiment	454:484	An L9(34) orthogonal experiment was performed to establish a protocol for calli induction as follows: the optimal explant is stems with nodes and the optimum medium formula is MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L to obtain callus for experimental use.
33963244	9	73	theme	triterpenoid	1721:1732	arg1	biosynthesis					1743:1754	triterpenoid saponins biosynthesis	1721:1754	triterpenoid saponins biosynthesis in P. grandiflorus	1721:1773	This study will help uncover the molecular mechanism of triterpenoid saponins biosynthesis in P. grandiflorus.
33963244	9	74	from	P. grandiflorus	1759:1773	arg1	mechanism					1708:1716	the molecular mechanism	1694:1716	the molecular mechanism of triterpenoid saponins biosynthesis in P. grandiflorus	1694:1773	This study will help uncover the molecular mechanism of triterpenoid saponins biosynthesis in P. grandiflorus.
33963244	7	75	theme	gene	1234:1237	arg1	sequences					1207:1215	putative sequences	1198:1215	putative sequences of β-Glucosidase gene converting platycoside E to platycodin D. Besides, we inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus	1198:1470	Associating platycodin D and platycoside E content with the expression level of genes involved in triterpenoid saponin biosynthesis between calli and leaves, three contigs were screened as putative sequences of β-Glucosidase gene converting platycoside E to platycodin D. Besides, we inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus.
33963244	7	75	theme	gene	1234:1237	arg1	contigs					1173:1179	three contigs	1167:1179	three contigs	1167:1179	Associating platycodin D and platycoside E content with the expression level of genes involved in triterpenoid saponin biosynthesis between calli and leaves, three contigs were screened as putative sequences of β-Glucosidase gene converting platycoside E to platycodin D. Besides, we inferred that some transcription factors can regulate the expression of key enzymes involved in triterpernoid saponins and polysaccharides biosynthesis pathway of P. grandiflorus.
33963244	6	76	theme	platycoside	916:926	arg1	E					928:928	platycoside E	916:928	platycoside E	916:928	While, platycoside E and total polysaccharide content of calli was found lower than that of leaves.
33963244	3	77	theme	optimum	604:610	arg1	0.5 mg/L					655:662	MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L	630:662	MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L to obtain callus for experimental use	630:700	An L9(34) orthogonal experiment was performed to establish a protocol for calli induction as follows: the optimal explant is stems with nodes and the optimum medium formula is MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L to obtain callus for experimental use.
33963244	3	77	theme	optimum	604:610	arg1	formula					619:625	the optimum medium formula	600:625	the optimum medium formula	600:625	An L9(34) orthogonal experiment was performed to establish a protocol for calli induction as follows: the optimal explant is stems with nodes and the optimum medium formula is MS + NAA 1.0 mg/L + 6-BA 0.5 mg/L to obtain callus for experimental use.
33963244	2	78	from	P. grandiflorus	437:451	arg1	existence					405:413	the potential existence	391:413	the potential existence of similar enzymes in P. grandiflorus	391:451	Studies have shown β-Glucosidase from bacteria can convert platycoside E to platycodin D, indicating the potential existence of similar enzymes in P. grandiflorus.
33963244	1	79	theme	Platycodin	149:158	arg1	saponins					201:208	two triterpenoid saponins	184:208	two triterpenoid saponins in Platycodon grandiflorus	184:235	Platycodin D and platycoside E are two triterpenoid saponins in Platycodon grandiflorus, differing only by two glycosyl groups structurally.
33963244	1	79	theme	Platycodin	149:158	arg1	D					160:160	Platycodin D	149:160	Platycodin D	149:160	Platycodin D and platycoside E are two triterpenoid saponins in Platycodon grandiflorus, differing only by two glycosyl groups structurally.
33963244	1	79	theme	Platycodin	149:158	arg1	E					178:178	platycoside E	166:178	platycoside E	166:178	Platycodin D and platycoside E are two triterpenoid saponins in Platycodon grandiflorus, differing only by two glycosyl groups structurally.
33963244	8	80	theme	platycoside	1538:1548	arg1	E					1550:1550	platycoside E	1538:1550	platycoside E	1538:1550	Totally, a candidate gene encoding enzyme involved in converting platycoside E to platycodin D, and putative genes involved in polysaccharide synthesis in P. grandiflorus had been identified.
32247159	1	0	theme	essential	300:308	arg1	oils					310:313	essential oils	300:313	essential oils	300:313	The effect of chitosan (C), chitosan enriched with thyme (CT) or rosemary (CR) essential oils, and potassium sorbate (PS) against superficial fungal growth was investigated in fermented sausages during 3 months of storage at 4 °C. For control groups, distilled water (DW) and acetic acid (AA) were used.
32247159	0	1	theme	sausages	196:203	arg1	composition					107:117	volatile composition	98:117	volatile composition	98:117	Contribution of surface application of chitosan-thyme and chitosan-rosemary essential oils to the volatile composition, microbial profile, and physicochemical and sensory quality of dry-fermented sausages during storage.
32247159	0	1	theme	sausages	196:203	arg1	profile					130:136	microbial profile	120:136	microbial profile	120:136	Contribution of surface application of chitosan-thyme and chitosan-rosemary essential oils to the volatile composition, microbial profile, and physicochemical and sensory quality of dry-fermented sausages during storage.
32247159	0	1	theme	sausages	196:203	arg1	quality					171:177	physicochemical and sensory quality	143:177	physicochemical and sensory quality	143:177	Contribution of surface application of chitosan-thyme and chitosan-rosemary essential oils to the volatile composition, microbial profile, and physicochemical and sensory quality of dry-fermented sausages during storage.
32247159	2	2	theme	lower	611:615	arg1	Gram					617:620	lower Gram	611:620	lower Gram	611:620	PS, C, CT and CR treatments inhibited fungal growth on casings while they resulted in lower Gram(+) catalase(+) cocci, Enterobacteriaceae, mold and yeast counts in sausages.
32247159	5	3	dep	DW	953:954	arg1	groups					963:968	groups	963:968	groups	963:968	Sausages coated with C, CT or CR had acceptable sensory attributes at the end of storage; however, DW and AA groups were rejected in the second and third month (p < .05), respectively, due to intense fungal growth which resulted in sensory defects.
32247159	1	4	dep	thyme	272:276	arg1	oils					310:313	essential oils	300:313	essential oils	300:313	The effect of chitosan (C), chitosan enriched with thyme (CT) or rosemary (CR) essential oils, and potassium sorbate (PS) against superficial fungal growth was investigated in fermented sausages during 3 months of storage at 4 °C. For control groups, distilled water (DW) and acetic acid (AA) were used.
32247159	0	5	theme	dry-fermented	182:194	arg1	sausages					196:203	dry-fermented sausages	182:203	dry-fermented sausages	182:203	Contribution of surface application of chitosan-thyme and chitosan-rosemary essential oils to the volatile composition, microbial profile, and physicochemical and sensory quality of dry-fermented sausages during storage.
32247159	5	6	theme	acceptable	891:900	arg1	attributes					910:919	acceptable sensory attributes	891:919	acceptable sensory attributes	891:919	Sausages coated with C, CT or CR had acceptable sensory attributes at the end of storage; however, DW and AA groups were rejected in the second and third month (p < .05), respectively, due to intense fungal growth which resulted in sensory defects.
32247159	1	7	theme	chitosan	249:256	arg1	effect					225:230	The effect	221:230	The effect of chitosan (C), chitosan enriched with thyme (CT) or rosemary (CR) essential oils, and potassium sorbate (PS) against superficial fungal growth	221:375	The effect of chitosan (C), chitosan enriched with thyme (CT) or rosemary (CR) essential oils, and potassium sorbate (PS) against superficial fungal growth was investigated in fermented sausages during 3 months of storage at 4 °C. For control groups, distilled water (DW) and acetic acid (AA) were used.
32247159	1	8	theme	fermented	397:405	arg1	sausages					407:414	fermented sausages during 3 months of storage at 4 °C. For control groups, distilled water (DW) and acetic acid (AA) were used	397:522	fermented sausages during 3 months of storage at 4 °C. For control groups, distilled water (DW) and acetic acid (AA) were used	397:522	The effect of chitosan (C), chitosan enriched with thyme (CT) or rosemary (CR) essential oils, and potassium sorbate (PS) against superficial fungal growth was investigated in fermented sausages during 3 months of storage at 4 °C. For control groups, distilled water (DW) and acetic acid (AA) were used.
32247159	5	9	theme	second	991:996	arg1	month					1008:1012	the second and third month	987:1012	the second and third month (p < .05)	987:1022	Sausages coated with C, CT or CR had acceptable sensory attributes at the end of storage; however, DW and AA groups were rejected in the second and third month (p < .05), respectively, due to intense fungal growth which resulted in sensory defects.
32247159	5	9	theme	second	991:996	arg1	p < .05					1015:1021	p < .05	1015:1021	p < .05	1015:1021	Sausages coated with C, CT or CR had acceptable sensory attributes at the end of storage; however, DW and AA groups were rejected in the second and third month (p < .05), respectively, due to intense fungal growth which resulted in sensory defects.
32247159	0	10	theme	volatile	98:105	arg1	composition					107:117	volatile composition	98:117	volatile composition	98:117	Contribution of surface application of chitosan-thyme and chitosan-rosemary essential oils to the volatile composition, microbial profile, and physicochemical and sensory quality of dry-fermented sausages during storage.
32247159	5	11	theme	sensory	902:908	arg1	attributes					910:919	acceptable sensory attributes	891:919	acceptable sensory attributes	891:919	Sausages coated with C, CT or CR had acceptable sensory attributes at the end of storage; however, DW and AA groups were rejected in the second and third month (p < .05), respectively, due to intense fungal growth which resulted in sensory defects.
32247159	2	12	dep	PS	525:526	arg1	treatments					542:551	treatments	542:551	treatments	542:551	PS, C, CT and CR treatments inhibited fungal growth on casings while they resulted in lower Gram(+) catalase(+) cocci, Enterobacteriaceae, mold and yeast counts in sausages.
32247159	5	13	theme	sensory	1086:1092	arg1	defects					1094:1100	sensory defects	1086:1100	sensory defects	1086:1100	Sausages coated with C, CT or CR had acceptable sensory attributes at the end of storage; however, DW and AA groups were rejected in the second and third month (p < .05), respectively, due to intense fungal growth which resulted in sensory defects.
32247159	0	14	theme	microbial	120:128	arg1	profile					130:136	microbial profile	120:136	microbial profile	120:136	Contribution of surface application of chitosan-thyme and chitosan-rosemary essential oils to the volatile composition, microbial profile, and physicochemical and sensory quality of dry-fermented sausages during storage.
32247159	1	15	theme	potassium	320:328	arg1	PS					339:340	PS	339:340	PS	339:340	The effect of chitosan (C), chitosan enriched with thyme (CT) or rosemary (CR) essential oils, and potassium sorbate (PS) against superficial fungal growth was investigated in fermented sausages during 3 months of storage at 4 °C. For control groups, distilled water (DW) and acetic acid (AA) were used.
32247159	1	15	theme	potassium	320:328	arg1	sorbate					330:336	potassium sorbate	320:336	potassium sorbate (PS)	320:341	The effect of chitosan (C), chitosan enriched with thyme (CT) or rosemary (CR) essential oils, and potassium sorbate (PS) against superficial fungal growth was investigated in fermented sausages during 3 months of storage at 4 °C. For control groups, distilled water (DW) and acetic acid (AA) were used.
32247159	5	16	theme	intense	1046:1052	arg1	growth					1061:1066	intense fungal growth	1046:1066	intense fungal growth which resulted in sensory defects	1046:1100	Sausages coated with C, CT or CR had acceptable sensory attributes at the end of storage; however, DW and AA groups were rejected in the second and third month (p < .05), respectively, due to intense fungal growth which resulted in sensory defects.
32247159	1	17	theme	sorbate	330:336	arg1	effect					225:230	The effect	221:230	The effect of chitosan (C), chitosan enriched with thyme (CT) or rosemary (CR) essential oils, and potassium sorbate (PS) against superficial fungal growth	221:375	The effect of chitosan (C), chitosan enriched with thyme (CT) or rosemary (CR) essential oils, and potassium sorbate (PS) against superficial fungal growth was investigated in fermented sausages during 3 months of storage at 4 °C. For control groups, distilled water (DW) and acetic acid (AA) were used.
32247159	2	18	theme	catalase	625:632	arg1	cocci					637:641	lower Gram(+) catalase(+) cocci	611:641	lower Gram(+) catalase(+) cocci	611:641	PS, C, CT and CR treatments inhibited fungal growth on casings while they resulted in lower Gram(+) catalase(+) cocci, Enterobacteriaceae, mold and yeast counts in sausages.
32247159	2	18	theme	catalase	625:632	arg1	mold					664:667	mold	664:667	mold	664:667	PS, C, CT and CR treatments inhibited fungal growth on casings while they resulted in lower Gram(+) catalase(+) cocci, Enterobacteriaceae, mold and yeast counts in sausages.
32247159	2	18	theme	catalase	625:632	arg1	counts					679:684	yeast counts	673:684	yeast counts	673:684	PS, C, CT and CR treatments inhibited fungal growth on casings while they resulted in lower Gram(+) catalase(+) cocci, Enterobacteriaceae, mold and yeast counts in sausages.
32247159	2	18	theme	catalase	625:632	arg1	Enterobacteriaceae					644:661	Enterobacteriaceae	644:661	Enterobacteriaceae	644:661	PS, C, CT and CR treatments inhibited fungal growth on casings while they resulted in lower Gram(+) catalase(+) cocci, Enterobacteriaceae, mold and yeast counts in sausages.
32247159	4	19	theme	volatile	780:787	arg1	compounds					789:797	44 and 64 volatile compounds	770:797	44 and 64 volatile compounds	770:797	A total of 44 and 64 volatile compounds were identified in sausages and casings, respectively.
32247159	1	20	theme	acetic	497:502	arg1	AA					510:511	AA	510:511	AA	510:511	The effect of chitosan (C), chitosan enriched with thyme (CT) or rosemary (CR) essential oils, and potassium sorbate (PS) against superficial fungal growth was investigated in fermented sausages during 3 months of storage at 4 °C. For control groups, distilled water (DW) and acetic acid (AA) were used.
32247159	1	20	theme	acetic	497:502	arg1	acid					504:507	acetic acid	497:507	acetic acid (AA)	497:512	The effect of chitosan (C), chitosan enriched with thyme (CT) or rosemary (CR) essential oils, and potassium sorbate (PS) against superficial fungal growth was investigated in fermented sausages during 3 months of storage at 4 °C. For control groups, distilled water (DW) and acetic acid (AA) were used.
32247159	5	21	contain	had	887:889	arg1	Sausages					854:861	Sausages	854:861	Sausages coated with C, CT or CR	854:885	Sausages coated with C, CT or CR had acceptable sensory attributes at the end of storage; however, DW and AA groups were rejected in the second and third month (p < .05), respectively, due to intense fungal growth which resulted in sensory defects.
32247159	5	21	contain	had	887:889	arg2	attributes					910:919	acceptable sensory attributes	891:919	acceptable sensory attributes	891:919	Sausages coated with C, CT or CR had acceptable sensory attributes at the end of storage; however, DW and AA groups were rejected in the second and third month (p < .05), respectively, due to intense fungal growth which resulted in sensory defects.
32247159	1	22	used	used	519:522	arg2	DW					489:490	DW	489:490	DW	489:490	The effect of chitosan (C), chitosan enriched with thyme (CT) or rosemary (CR) essential oils, and potassium sorbate (PS) against superficial fungal growth was investigated in fermented sausages during 3 months of storage at 4 °C. For control groups, distilled water (DW) and acetic acid (AA) were used.
32247159	1	22	used	used	519:522	arg2	water					482:486	distilled water	472:486	distilled water (DW)	472:491	The effect of chitosan (C), chitosan enriched with thyme (CT) or rosemary (CR) essential oils, and potassium sorbate (PS) against superficial fungal growth was investigated in fermented sausages during 3 months of storage at 4 °C. For control groups, distilled water (DW) and acetic acid (AA) were used.
32247159	1	22	used	used	519:522	arg2	acid					504:507	acetic acid	497:507	acetic acid (AA)	497:512	The effect of chitosan (C), chitosan enriched with thyme (CT) or rosemary (CR) essential oils, and potassium sorbate (PS) against superficial fungal growth was investigated in fermented sausages during 3 months of storage at 4 °C. For control groups, distilled water (DW) and acetic acid (AA) were used.
32247159	1	22	used	used	519:522	arg2	AA					510:511	AA	510:511	AA	510:511	The effect of chitosan (C), chitosan enriched with thyme (CT) or rosemary (CR) essential oils, and potassium sorbate (PS) against superficial fungal growth was investigated in fermented sausages during 3 months of storage at 4 °C. For control groups, distilled water (DW) and acetic acid (AA) were used.
32247159	0	23	theme	application	24:34	arg1	Contribution					0:11	Contribution	0:11	Contribution of surface application of chitosan-thyme and chitosan-rosemary essential oils to the volatile composition, microbial profile, and physicochemical and sensory quality of dry-fermented sausages during storage.	0:219	Contribution of surface application of chitosan-thyme and chitosan-rosemary essential oils to the volatile composition, microbial profile, and physicochemical and sensory quality of dry-fermented sausages during storage.
32247159	1	24	theme	storage	435:441	arg1	3 months					423:430	3 months	423:430	3 months of storage at 4 °C. For control groups	423:469	The effect of chitosan (C), chitosan enriched with thyme (CT) or rosemary (CR) essential oils, and potassium sorbate (PS) against superficial fungal growth was investigated in fermented sausages during 3 months of storage at 4 °C. For control groups, distilled water (DW) and acetic acid (AA) were used.
32247159	2	25	theme	fungal	563:568	arg1	growth					570:575	fungal growth	563:575	fungal growth on casings	563:586	PS, C, CT and CR treatments inhibited fungal growth on casings while they resulted in lower Gram(+) catalase(+) cocci, Enterobacteriaceae, mold and yeast counts in sausages.
32247159	0	26	theme	surface	16:22	arg1	application					24:34	surface application	16:34	surface application of chitosan-thyme and chitosan-rosemary essential oils	16:89	Contribution of surface application of chitosan-thyme and chitosan-rosemary essential oils to the volatile composition, microbial profile, and physicochemical and sensory quality of dry-fermented sausages during storage.
32247159	0	27	theme	chitosan-thyme	39:52	arg1	oils					86:89	chitosan-thyme and chitosan-rosemary essential oils	39:89	chitosan-thyme and chitosan-rosemary essential oils	39:89	Contribution of surface application of chitosan-thyme and chitosan-rosemary essential oils to the volatile composition, microbial profile, and physicochemical and sensory quality of dry-fermented sausages during storage.
32247159	0	28	theme	physicochemical	143:157	arg1	quality					171:177	physicochemical and sensory quality	143:177	physicochemical and sensory quality	143:177	Contribution of surface application of chitosan-thyme and chitosan-rosemary essential oils to the volatile composition, microbial profile, and physicochemical and sensory quality of dry-fermented sausages during storage.
32247159	5	29	theme	fungal	1054:1059	arg1	growth					1061:1066	intense fungal growth	1046:1066	intense fungal growth which resulted in sensory defects	1046:1100	Sausages coated with C, CT or CR had acceptable sensory attributes at the end of storage; however, DW and AA groups were rejected in the second and third month (p < .05), respectively, due to intense fungal growth which resulted in sensory defects.
32247159	1	30	from	4 °C.	446:450	arg1	storage					435:441	storage	435:441	storage at 4 °C. For control groups	435:469	The effect of chitosan (C), chitosan enriched with thyme (CT) or rosemary (CR) essential oils, and potassium sorbate (PS) against superficial fungal growth was investigated in fermented sausages during 3 months of storage at 4 °C. For control groups, distilled water (DW) and acetic acid (AA) were used.
32247159	1	30	from	4 °C.	446:450	arg1	3 months					423:430	3 months	423:430	3 months of storage at 4 °C. For control groups	423:469	The effect of chitosan (C), chitosan enriched with thyme (CT) or rosemary (CR) essential oils, and potassium sorbate (PS) against superficial fungal growth was investigated in fermented sausages during 3 months of storage at 4 °C. For control groups, distilled water (DW) and acetic acid (AA) were used.
32247159	2	31	theme	yeast	673:677	arg1	cocci					637:641	lower Gram(+) catalase(+) cocci	611:641	lower Gram(+) catalase(+) cocci	611:641	PS, C, CT and CR treatments inhibited fungal growth on casings while they resulted in lower Gram(+) catalase(+) cocci, Enterobacteriaceae, mold and yeast counts in sausages.
32247159	2	31	theme	yeast	673:677	arg1	counts					679:684	yeast counts	673:684	yeast counts	673:684	PS, C, CT and CR treatments inhibited fungal growth on casings while they resulted in lower Gram(+) catalase(+) cocci, Enterobacteriaceae, mold and yeast counts in sausages.
32247159	0	32	theme	chitosan-rosemary	58:74	arg1	oils					86:89	chitosan-thyme and chitosan-rosemary essential oils	39:89	chitosan-thyme and chitosan-rosemary essential oils	39:89	Contribution of surface application of chitosan-thyme and chitosan-rosemary essential oils to the volatile composition, microbial profile, and physicochemical and sensory quality of dry-fermented sausages during storage.
32247159	0	33	theme	sensory	163:169	arg1	quality					171:177	physicochemical and sensory quality	143:177	physicochemical and sensory quality	143:177	Contribution of surface application of chitosan-thyme and chitosan-rosemary essential oils to the volatile composition, microbial profile, and physicochemical and sensory quality of dry-fermented sausages during storage.
32247159	1	34	theme	superficial	351:361	arg1	growth					370:375	superficial fungal growth	351:375	superficial fungal growth	351:375	The effect of chitosan (C), chitosan enriched with thyme (CT) or rosemary (CR) essential oils, and potassium sorbate (PS) against superficial fungal growth was investigated in fermented sausages during 3 months of storage at 4 °C. For control groups, distilled water (DW) and acetic acid (AA) were used.
32247159	5	35	theme	storage	935:941	arg1	end					928:930	the end	924:930	the end of storage	924:941	Sausages coated with C, CT or CR had acceptable sensory attributes at the end of storage; however, DW and AA groups were rejected in the second and third month (p < .05), respectively, due to intense fungal growth which resulted in sensory defects.
32247159	1	36	theme	fungal	363:368	arg1	growth					370:375	superficial fungal growth	351:375	superficial fungal growth	351:375	The effect of chitosan (C), chitosan enriched with thyme (CT) or rosemary (CR) essential oils, and potassium sorbate (PS) against superficial fungal growth was investigated in fermented sausages during 3 months of storage at 4 °C. For control groups, distilled water (DW) and acetic acid (AA) were used.
32247159	4	37	theme	compounds	789:797	arg1	total					761:765	A total	759:765	A total of 44 and 64 volatile compounds	759:797	A total of 44 and 64 volatile compounds were identified in sausages and casings, respectively.
32247159	1	38	theme	control	456:462	arg1	groups					464:469	control groups	456:469	control groups	456:469	The effect of chitosan (C), chitosan enriched with thyme (CT) or rosemary (CR) essential oils, and potassium sorbate (PS) against superficial fungal growth was investigated in fermented sausages during 3 months of storage at 4 °C. For control groups, distilled water (DW) and acetic acid (AA) were used.
32247159	1	39	dep	sausages	407:414	arg1	used					519:522	used	519:522	were used	514:522	The effect of chitosan (C), chitosan enriched with thyme (CT) or rosemary (CR) essential oils, and potassium sorbate (PS) against superficial fungal growth was investigated in fermented sausages during 3 months of storage at 4 °C. For control groups, distilled water (DW) and acetic acid (AA) were used.
32247159	1	40	from	3 months	423:430	arg1	4 °C.					446:450	4 °C.	446:450	4 °C. For control groups	446:469	The effect of chitosan (C), chitosan enriched with thyme (CT) or rosemary (CR) essential oils, and potassium sorbate (PS) against superficial fungal growth was investigated in fermented sausages during 3 months of storage at 4 °C. For control groups, distilled water (DW) and acetic acid (AA) were used.
32247159	1	41	theme	chitosan	235:242	arg1	effect					225:230	The effect	221:230	The effect of chitosan (C), chitosan enriched with thyme (CT) or rosemary (CR) essential oils, and potassium sorbate (PS) against superficial fungal growth	221:375	The effect of chitosan (C), chitosan enriched with thyme (CT) or rosemary (CR) essential oils, and potassium sorbate (PS) against superficial fungal growth was investigated in fermented sausages during 3 months of storage at 4 °C. For control groups, distilled water (DW) and acetic acid (AA) were used.
32247159	3	42	theme	Lower	699:703	arg1	values					711:716	Lower TBARS values	699:716	Lower TBARS values	699:716	Lower TBARS values were determined for CT and CR (p < .05).
32247159	0	43	theme	oils	86:89	arg1	application					24:34	surface application	16:34	surface application of chitosan-thyme and chitosan-rosemary essential oils	16:89	Contribution of surface application of chitosan-thyme and chitosan-rosemary essential oils to the volatile composition, microbial profile, and physicochemical and sensory quality of dry-fermented sausages during storage.
32247159	1	44	theme	distilled	472:480	arg1	DW					489:490	DW	489:490	DW	489:490	The effect of chitosan (C), chitosan enriched with thyme (CT) or rosemary (CR) essential oils, and potassium sorbate (PS) against superficial fungal growth was investigated in fermented sausages during 3 months of storage at 4 °C. For control groups, distilled water (DW) and acetic acid (AA) were used.
32247159	1	44	theme	distilled	472:480	arg1	water					482:486	distilled water	472:486	distilled water (DW)	472:491	The effect of chitosan (C), chitosan enriched with thyme (CT) or rosemary (CR) essential oils, and potassium sorbate (PS) against superficial fungal growth was investigated in fermented sausages during 3 months of storage at 4 °C. For control groups, distilled water (DW) and acetic acid (AA) were used.
32247159	2	45	from	growth	570:575	arg1	casings					580:586	casings	580:586	casings	580:586	PS, C, CT and CR treatments inhibited fungal growth on casings while they resulted in lower Gram(+) catalase(+) cocci, Enterobacteriaceae, mold and yeast counts in sausages.
32247159	3	46	theme	TBARS	705:709	arg1	values					711:716	Lower TBARS values	699:716	Lower TBARS values	699:716	Lower TBARS values were determined for CT and CR (p < .05).
32247159	0	47	theme	essential	76:84	arg1	oils					86:89	chitosan-thyme and chitosan-rosemary essential oils	39:89	chitosan-thyme and chitosan-rosemary essential oils	39:89	Contribution of surface application of chitosan-thyme and chitosan-rosemary essential oils to the volatile composition, microbial profile, and physicochemical and sensory quality of dry-fermented sausages during storage.
32247159	2	48	theme	Gram	617:620	arg1	cocci					637:641	lower Gram(+) catalase(+) cocci	611:641	lower Gram(+) catalase(+) cocci	611:641	PS, C, CT and CR treatments inhibited fungal growth on casings while they resulted in lower Gram(+) catalase(+) cocci, Enterobacteriaceae, mold and yeast counts in sausages.
32247159	2	48	theme	Gram	617:620	arg1	mold					664:667	mold	664:667	mold	664:667	PS, C, CT and CR treatments inhibited fungal growth on casings while they resulted in lower Gram(+) catalase(+) cocci, Enterobacteriaceae, mold and yeast counts in sausages.
32247159	2	48	theme	Gram	617:620	arg1	counts					679:684	yeast counts	673:684	yeast counts	673:684	PS, C, CT and CR treatments inhibited fungal growth on casings while they resulted in lower Gram(+) catalase(+) cocci, Enterobacteriaceae, mold and yeast counts in sausages.
32247159	2	48	theme	Gram	617:620	arg1	Enterobacteriaceae					644:661	Enterobacteriaceae	644:661	Enterobacteriaceae	644:661	PS, C, CT and CR treatments inhibited fungal growth on casings while they resulted in lower Gram(+) catalase(+) cocci, Enterobacteriaceae, mold and yeast counts in sausages.
32247159	5	49	theme	third	1002:1006	arg1	month					1008:1012	the second and third month	987:1012	the second and third month (p < .05)	987:1022	Sausages coated with C, CT or CR had acceptable sensory attributes at the end of storage; however, DW and AA groups were rejected in the second and third month (p < .05), respectively, due to intense fungal growth which resulted in sensory defects.
32247159	5	49	theme	third	1002:1006	arg1	p < .05					1015:1021	p < .05	1015:1021	p < .05	1015:1021	Sausages coated with C, CT or CR had acceptable sensory attributes at the end of storage; however, DW and AA groups were rejected in the second and third month (p < .05), respectively, due to intense fungal growth which resulted in sensory defects.
32648307	1	0	dep	meat	297:300	arg1	fat					328:330	fat	328:330	fat	328:330	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	0	1	theme	Bologna	67:73	arg1	sausage					75:81	Bologna sausage	67:81	Bologna sausage	67:81	Using inulin-based emulsion gels as fat substitute in salt reduced Bologna sausage.
32648307	1	2	theme	pork	303:306	arg1	meat					308:311	pork meat	303:311	pork meat	303:311	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	2	3	dep	°C.	410:412	arg1	RESULTS					414:420	RESULTS	414:420	RESULTS A reduction of 11 to 34%	414:445	Technological and microbiological properties were evaluated for 60 days at 4 °C. RESULTS A reduction of 11 to 34% and 35 to 45% of fat and sodium were obtained in reformulated products, respectively.
32648307	1	4	dep	BACKGROUND	84:93	arg1	applied					186:192	applied	186:192	was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat	182:330	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	6	5	theme	elastic	874:880	arg1	samples					906:912	Softer, more elastic, cohesive and resilient samples	861:912	Softer, more elastic, cohesive and resilient samples with a higher intensity of lipid oxidation (P < 0.05)	861:966	Softer, more elastic, cohesive and resilient samples with a higher intensity of lipid oxidation (P < 0.05) were observed in EG added sausages.
32648307	8	6	theme	EGs	1209:1211	arg1	incorporation					1188:1200	the incorporation	1184:1200	the incorporation of the EGs	1184:1211	Sensory evaluation showed that the incorporation of the EGs resulted in acceptable scores.
32648307	2	7	theme	reformulated	496:507	arg1	products					509:516	reformulated products	496:516	reformulated products	496:516	Technological and microbiological properties were evaluated for 60 days at 4 °C. RESULTS A reduction of 11 to 34% and 35 to 45% of fat and sodium were obtained in reformulated products, respectively.
32648307	4	8	theme	*	709:709	arg1	values					731:736	reduced a* (redness/greenness) values	700:736	reduced a* (redness/greenness) values comparing to control treatment	700:767	The addition of EG in Bologna increased L* (lightness) values and reduced a* (redness/greenness) values comparing to control treatment.
32648307	9	9	theme	fatty	1380:1384	arg1	acids					1386:1390	better fatty acids	1373:1390	better fatty acids composition and stable to chilled storage	1373:1432	CONCLUSION These results suggest that inulin-based EG is a potential fat substitute for developing healthier meat products, with better fatty acids composition and stable to chilled storage.
32648307	1	10	theme	high-fiber	97:106	arg1	EG					122:123	EG	122:123	EG	122:123	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	1	10	theme	high-fiber	97:106	arg1	gel					117:119	A high-fiber emulsion gel	95:119	A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil	95:180	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	1	10	theme	high-fiber	97:106	arg1	replacer					208:215	animal fat replacer	197:215	animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat	197:330	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	1	11	theme	reduced	220:226	arg1	salt					228:231	salt	228:231	salt	228:231	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	2	12	dep	45	457:458	arg1	to					454:455	to	454:455	to	454:455	Technological and microbiological properties were evaluated for 60 days at 4 °C. RESULTS A reduction of 11 to 34% and 35 to 45% of fat and sodium were obtained in reformulated products, respectively.
32648307	4	13	theme	control	751:757	arg1	treatment					759:767	control treatment	751:767	control treatment	751:767	The addition of EG in Bologna increased L* (lightness) values and reduced a* (redness/greenness) values comparing to control treatment.
32648307	5	14	theme	EG	857:858	arg1	presence					845:852	the presence	841:852	the presence of EG	841:858	Microstructural properties of sausages exhibited a denser network with the presence of EG.
32648307	9	15	theme	meat	1353:1356	arg1	products					1358:1365	healthier meat products	1343:1365	healthier meat products	1343:1365	CONCLUSION These results suggest that inulin-based EG is a potential fat substitute for developing healthier meat products, with better fatty acids composition and stable to chilled storage.
32648307	8	16	theme	acceptable	1225:1234	arg1	scores					1236:1241	acceptable scores	1225:1241	acceptable scores	1225:1241	Sensory evaluation showed that the incorporation of the EGs resulted in acceptable scores.
32648307	5	17	theme	sausages	800:807	arg1	properties					786:795	Microstructural properties	770:795	Microstructural properties of sausages	770:807	Microstructural properties of sausages exhibited a denser network with the presence of EG.
32648307	6	18	theme	higher	921:926	arg1	intensity					928:936	a higher intensity	919:936	a higher intensity of lipid oxidation (P < 0.05)	919:966	Softer, more elastic, cohesive and resilient samples with a higher intensity of lipid oxidation (P < 0.05) were observed in EG added sausages.
32648307	4	19	theme	*	675:675	arg1	values					689:694	L* (lightness) values	674:694	L* (lightness) values	674:694	The addition of EG in Bologna increased L* (lightness) values and reduced a* (redness/greenness) values comparing to control treatment.
32648307	7	20	theme	salt	1147:1150	arg1	reduction					1126:1134	reduction	1126:1134	reduction of fat and salt	1126:1150	The nuclear magnetic resonance (NMR) data shows that the presence of EG recovers the matrix that has been weakened due to reduction of fat and salt.
32648307	1	21	theme	soy	145:147	arg1	isolate					157:163	soy protein isolate	145:163	soy protein isolate	145:163	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	2	22	theme	%	445:445	arg1	reduction					424:432	A reduction	422:432	A reduction of 11 to 34%	422:445	Technological and microbiological properties were evaluated for 60 days at 4 °C. RESULTS A reduction of 11 to 34% and 35 to 45% of fat and sodium were obtained in reformulated products, respectively.
32648307	0	23	from	fat	36:38	arg1	salt					54:57	salt	54:57	salt	54:57	Using inulin-based emulsion gels as fat substitute in salt reduced Bologna sausage.
32648307	9	24	dep	acids	1386:1390	arg1	composition					1392:1402	composition	1392:1402	composition	1392:1402	CONCLUSION These results suggest that inulin-based EG is a potential fat substitute for developing healthier meat products, with better fatty acids composition and stable to chilled storage.
32648307	9	24	dep	acids	1386:1390	arg1	stable					1408:1413	stable	1408:1413	stable	1408:1413	CONCLUSION These results suggest that inulin-based EG is a potential fat substitute for developing healthier meat products, with better fatty acids composition and stable to chilled storage.
32648307	4	25	theme	L	674:674	arg1	lightness					678:686	lightness	678:686	lightness	678:686	The addition of EG in Bologna increased L* (lightness) values and reduced a* (redness/greenness) values comparing to control treatment.
32648307	4	25	theme	L	674:674	arg1	*					675:675	L*	674:675	L* (lightness) values	674:694	The addition of EG in Bologna increased L* (lightness) values and reduced a* (redness/greenness) values comparing to control treatment.
32648307	6	26	with	samples	906:912	arg1	intensity					928:936	a higher intensity	919:936	a higher intensity of lipid oxidation (P < 0.05)	919:966	Softer, more elastic, cohesive and resilient samples with a higher intensity of lipid oxidation (P < 0.05) were observed in EG added sausages.
32648307	1	27	theme	protein	149:155	arg1	isolate					157:163	soy protein isolate	145:163	soy protein isolate	145:163	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	0	28	theme	inulin-based	6:17	arg1	gels					28:31	inulin-based emulsion gels	6:31	inulin-based emulsion gels	6:31	Using inulin-based emulsion gels as fat substitute in salt reduced Bologna sausage.
32648307	3	29	from	increase	536:543	arg1	content					554:560	fiber content	548:560	fiber content	548:560	An increase in fiber content and polyunsaturated fatty acid was noticed in the formulations with EG.
32648307	3	29	from	increase	536:543	arg1	acid					588:591	polyunsaturated fatty acid	566:591	polyunsaturated fatty acid	566:591	An increase in fiber content and polyunsaturated fatty acid was noticed in the formulations with EG.
32648307	6	30	theme	lipid	941:945	arg1	P < 0.05					958:965	P < 0.05	958:965	P < 0.05	958:965	Softer, more elastic, cohesive and resilient samples with a higher intensity of lipid oxidation (P < 0.05) were observed in EG added sausages.
32648307	6	30	theme	lipid	941:945	arg1	oxidation					947:955	lipid oxidation	941:955	lipid oxidation (P < 0.05)	941:966	Softer, more elastic, cohesive and resilient samples with a higher intensity of lipid oxidation (P < 0.05) were observed in EG added sausages.
32648307	6	31	theme	Softer	861:866	arg1	samples					906:912	Softer, more elastic, cohesive and resilient samples	861:912	Softer, more elastic, cohesive and resilient samples with a higher intensity of lipid oxidation (P < 0.05)	861:966	Softer, more elastic, cohesive and resilient samples with a higher intensity of lipid oxidation (P < 0.05) were observed in EG added sausages.
32648307	3	32	with	formulations	612:623	arg1	EG					630:631	EG	630:631	EG	630:631	An increase in fiber content and polyunsaturated fatty acid was noticed in the formulations with EG.
32648307	6	33	theme	oxidation	947:955	arg1	intensity					928:936	a higher intensity	919:936	a higher intensity of lipid oxidation (P < 0.05)	919:966	Softer, more elastic, cohesive and resilient samples with a higher intensity of lipid oxidation (P < 0.05) were observed in EG added sausages.
32648307	2	34	theme	fat	464:466	arg1	sodium					472:477	sodium	472:477	sodium	472:477	Technological and microbiological properties were evaluated for 60 days at 4 °C. RESULTS A reduction of 11 to 34% and 35 to 45% of fat and sodium were obtained in reformulated products, respectively.
32648307	2	34	theme	fat	464:466	arg1	fat					464:466	fat	464:466	fat	464:466	Technological and microbiological properties were evaluated for 60 days at 4 °C. RESULTS A reduction of 11 to 34% and 35 to 45% of fat and sodium were obtained in reformulated products, respectively.
32648307	2	34	theme	fat	464:466	arg1	%					459:459	35 to 45%	451:459	35 to 45% of fat and sodium	451:477	Technological and microbiological properties were evaluated for 60 days at 4 °C. RESULTS A reduction of 11 to 34% and 35 to 45% of fat and sodium were obtained in reformulated products, respectively.
32648307	4	35	from	addition	638:645	arg1	Bologna					656:662	Bologna	656:662	Bologna	656:662	The addition of EG in Bologna increased L* (lightness) values and reduced a* (redness/greenness) values comparing to control treatment.
32648307	8	36	theme	Sensory	1153:1159	arg1	evaluation					1161:1170	Sensory evaluation	1153:1170	Sensory evaluation	1153:1170	Sensory evaluation showed that the incorporation of the EGs resulted in acceptable scores.
32648307	2	37	dep	34	443:444	arg1	to					440:441	to	440:441	to	440:441	Technological and microbiological properties were evaluated for 60 days at 4 °C. RESULTS A reduction of 11 to 34% and 35 to 45% of fat and sodium were obtained in reformulated products, respectively.
32648307	7	38	theme	fat	1139:1141	arg1	reduction					1126:1134	reduction	1126:1134	reduction of fat and salt	1126:1150	The nuclear magnetic resonance (NMR) data shows that the presence of EG recovers the matrix that has been weakened due to reduction of fat and salt.
32648307	0	39	theme	emulsion	19:26	arg1	gels					28:31	inulin-based emulsion gels	6:31	inulin-based emulsion gels	6:31	Using inulin-based emulsion gels as fat substitute in salt reduced Bologna sausage.
32648307	1	40	theme	Bologna	241:247	arg1	sausage					249:255	Bologna sausage	241:255	Bologna sausage	241:255	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	9	41	theme	healthier	1343:1351	arg1	products					1358:1365	healthier meat products	1343:1365	healthier meat products	1343:1365	CONCLUSION These results suggest that inulin-based EG is a potential fat substitute for developing healthier meat products, with better fatty acids composition and stable to chilled storage.
32648307	1	42	contain	containing	257:266	arg2	meat					297:300	mechanically deboned chicken meat	268:300	mechanically deboned chicken meat	268:300	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	1	42	contain	containing	257:266	arg2	pork					318:321	pork	318:321	pork	318:321	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	1	42	contain	containing	257:266	arg2	meat					308:311	pork meat	303:311	pork meat	303:311	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	1	42	contain	containing	257:266	arg1	salt					228:231	salt	228:231	salt	228:231	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	9	43	theme	better	1373:1378	arg1	acids					1386:1390	better fatty acids	1373:1390	better fatty acids composition and stable to chilled storage	1373:1432	CONCLUSION These results suggest that inulin-based EG is a potential fat substitute for developing healthier meat products, with better fatty acids composition and stable to chilled storage.
32648307	1	44	theme	soybean	170:176	arg1	oil					178:180	soybean oil	170:180	soybean oil	170:180	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	7	45	theme	nuclear	1008:1014	arg1	NMR					1036:1038	NMR	1036:1038	NMR	1036:1038	The nuclear magnetic resonance (NMR) data shows that the presence of EG recovers the matrix that has been weakened due to reduction of fat and salt.
32648307	7	45	theme	nuclear	1008:1014	arg1	resonance					1025:1033	nuclear magnetic resonance	1008:1033	The nuclear magnetic resonance (NMR) data	1004:1044	The nuclear magnetic resonance (NMR) data shows that the presence of EG recovers the matrix that has been weakened due to reduction of fat and salt.
32648307	1	46	from	replacer	208:215	arg1	fat					237:239	fat	237:239	fat	237:239	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	1	46	from	replacer	208:215	arg1	salt					228:231	salt	228:231	salt	228:231	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	6	47	located	observed	973:980	arg2	samples					906:912	Softer, more elastic, cohesive and resilient samples	861:912	Softer, more elastic, cohesive and resilient samples with a higher intensity of lipid oxidation (P < 0.05)	861:966	Softer, more elastic, cohesive and resilient samples with a higher intensity of lipid oxidation (P < 0.05) were observed in EG added sausages.
32648307	6	47	located	observed	973:980	arg1	sausages					994:1001	EG added sausages	985:1001	EG added sausages	985:1001	Softer, more elastic, cohesive and resilient samples with a higher intensity of lipid oxidation (P < 0.05) were observed in EG added sausages.
32648307	4	48	theme	reduced	700:706	arg1	values					731:736	reduced a* (redness/greenness) values	700:736	reduced a* (redness/greenness) values comparing to control treatment	700:767	The addition of EG in Bologna increased L* (lightness) values and reduced a* (redness/greenness) values comparing to control treatment.
32648307	7	49	theme	magnetic	1016:1023	arg1	NMR					1036:1038	NMR	1036:1038	NMR	1036:1038	The nuclear magnetic resonance (NMR) data shows that the presence of EG recovers the matrix that has been weakened due to reduction of fat and salt.
32648307	7	49	theme	magnetic	1016:1023	arg1	resonance					1025:1033	nuclear magnetic resonance	1008:1033	The nuclear magnetic resonance (NMR) data	1004:1044	The nuclear magnetic resonance (NMR) data shows that the presence of EG recovers the matrix that has been weakened due to reduction of fat and salt.
32648307	7	50	theme	resonance	1025:1033	arg1	data					1041:1044	The nuclear magnetic resonance (NMR) data	1004:1044	The nuclear magnetic resonance (NMR) data	1004:1044	The nuclear magnetic resonance (NMR) data shows that the presence of EG recovers the matrix that has been weakened due to reduction of fat and salt.
32648307	2	51	theme	microbiological	351:365	arg1	properties					367:376	Technological and microbiological properties	333:376	Technological and microbiological properties	333:376	Technological and microbiological properties were evaluated for 60 days at 4 °C. RESULTS A reduction of 11 to 34% and 35 to 45% of fat and sodium were obtained in reformulated products, respectively.
32648307	10	52	theme	Chemical	1453:1460	arg1	Industry					1462:1469	Chemical Industry	1453:1469	Chemical Industry	1453:1469	© 2020 Society of Chemical Industry.
32648307	9	53	theme	inulin-based	1282:1293	arg1	EG					1295:1296	inulin-based EG	1282:1296	inulin-based EG	1282:1296	CONCLUSION These results suggest that inulin-based EG is a potential fat substitute for developing healthier meat products, with better fatty acids composition and stable to chilled storage.
32648307	9	53	theme	inulin-based	1282:1293	arg1	fat					1313:1315	fat	1313:1315	fat	1313:1315	CONCLUSION These results suggest that inulin-based EG is a potential fat substitute for developing healthier meat products, with better fatty acids composition and stable to chilled storage.
32648307	3	54	theme	fiber	548:552	arg1	content					554:560	fiber content	548:560	fiber content	548:560	An increase in fiber content and polyunsaturated fatty acid was noticed in the formulations with EG.
32648307	0	55	dep	fat	36:38	arg1	substitute					40:49	substitute	40:49	substitute	40:49	Using inulin-based emulsion gels as fat substitute in salt reduced Bologna sausage.
32648307	1	56	contain	containing	126:135	arg2	oil					178:180	soybean oil	170:180	soybean oil	170:180	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	1	56	contain	containing	126:135	arg1	gel					117:119	A high-fiber emulsion gel	95:119	A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil	95:180	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	1	56	contain	containing	126:135	arg1	replacer					208:215	animal fat replacer	197:215	animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat	197:330	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	1	56	contain	containing	126:135	arg2	isolate					157:163	soy protein isolate	145:163	soy protein isolate	145:163	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	1	56	contain	containing	126:135	arg2	inulin					137:142	inulin	137:142	inulin	137:142	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	1	56	contain	containing	126:135	arg1	EG					122:123	EG	122:123	EG	122:123	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	1	57	theme	emulsion	108:115	arg1	EG					122:123	EG	122:123	EG	122:123	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	1	57	theme	emulsion	108:115	arg1	gel					117:119	A high-fiber emulsion gel	95:119	A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil	95:180	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	1	57	theme	emulsion	108:115	arg1	replacer					208:215	animal fat replacer	197:215	animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat	197:330	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	1	58	theme	deboned	281:287	arg1	meat					297:300	mechanically deboned chicken meat	268:300	mechanically deboned chicken meat	268:300	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	6	59	theme	added	988:992	arg1	sausages					994:1001	EG added sausages	985:1001	EG added sausages	985:1001	Softer, more elastic, cohesive and resilient samples with a higher intensity of lipid oxidation (P < 0.05) were observed in EG added sausages.
32648307	1	60	theme	chicken	289:295	arg1	meat					297:300	mechanically deboned chicken meat	268:300	mechanically deboned chicken meat	268:300	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	2	61	theme	sodium	472:477	arg1	sodium					472:477	sodium	472:477	sodium	472:477	Technological and microbiological properties were evaluated for 60 days at 4 °C. RESULTS A reduction of 11 to 34% and 35 to 45% of fat and sodium were obtained in reformulated products, respectively.
32648307	2	61	theme	sodium	472:477	arg1	fat					464:466	fat	464:466	fat	464:466	Technological and microbiological properties were evaluated for 60 days at 4 °C. RESULTS A reduction of 11 to 34% and 35 to 45% of fat and sodium were obtained in reformulated products, respectively.
32648307	2	61	theme	sodium	472:477	arg1	%					459:459	35 to 45%	451:459	35 to 45% of fat and sodium	451:477	Technological and microbiological properties were evaluated for 60 days at 4 °C. RESULTS A reduction of 11 to 34% and 35 to 45% of fat and sodium were obtained in reformulated products, respectively.
32648307	1	62	dep	salt	228:231	arg1	sausage					249:255	Bologna sausage	241:255	Bologna sausage	241:255	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	9	63	dep	fat	1313:1315	arg1	substitute					1317:1326	substitute	1317:1326	substitute for developing healthier meat products, with better fatty acids composition and stable to chilled storage	1317:1432	CONCLUSION These results suggest that inulin-based EG is a potential fat substitute for developing healthier meat products, with better fatty acids composition and stable to chilled storage.
32648307	6	64	theme	EG	985:986	arg1	sausages					994:1001	EG added sausages	985:1001	EG added sausages	985:1001	Softer, more elastic, cohesive and resilient samples with a higher intensity of lipid oxidation (P < 0.05) were observed in EG added sausages.
32648307	9	65	theme	chilled	1418:1424	arg1	storage					1426:1432	chilled storage	1418:1432	chilled storage	1418:1432	CONCLUSION These results suggest that inulin-based EG is a potential fat substitute for developing healthier meat products, with better fatty acids composition and stable to chilled storage.
32648307	3	66	theme	polyunsaturated	566:580	arg1	acid					588:591	polyunsaturated fatty acid	566:591	polyunsaturated fatty acid	566:591	An increase in fiber content and polyunsaturated fatty acid was noticed in the formulations with EG.
32648307	4	67	theme	EG	650:651	arg1	addition					638:645	The addition	634:645	The addition of EG in Bologna	634:662	The addition of EG in Bologna increased L* (lightness) values and reduced a* (redness/greenness) values comparing to control treatment.
32648307	5	68	theme	denser	821:826	arg1	network					828:834	a denser network	819:834	a denser network	819:834	Microstructural properties of sausages exhibited a denser network with the presence of EG.
32648307	6	69	theme	resilient	896:904	arg1	samples					906:912	Softer, more elastic, cohesive and resilient samples	861:912	Softer, more elastic, cohesive and resilient samples with a higher intensity of lipid oxidation (P < 0.05)	861:966	Softer, more elastic, cohesive and resilient samples with a higher intensity of lipid oxidation (P < 0.05) were observed in EG added sausages.
32648307	7	70	theme	EG	1073:1074	arg1	presence					1061:1068	the presence	1057:1068	the presence of EG	1057:1074	The nuclear magnetic resonance (NMR) data shows that the presence of EG recovers the matrix that has been weakened due to reduction of fat and salt.
32648307	3	71	theme	fatty	582:586	arg1	acid					588:591	polyunsaturated fatty acid	566:591	polyunsaturated fatty acid	566:591	An increase in fiber content and polyunsaturated fatty acid was noticed in the formulations with EG.
32648307	2	72	theme	Technological	333:345	arg1	properties					367:376	Technological and microbiological properties	333:376	Technological and microbiological properties	333:376	Technological and microbiological properties were evaluated for 60 days at 4 °C. RESULTS A reduction of 11 to 34% and 35 to 45% of fat and sodium were obtained in reformulated products, respectively.
32648307	1	73	theme	animal	197:202	arg1	gel					117:119	A high-fiber emulsion gel	95:119	A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil	95:180	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	1	73	theme	animal	197:202	arg1	replacer					208:215	animal fat replacer	197:215	animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat	197:330	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	9	74	dep	CONCLUSION	1244:1253	arg1	suggest					1269:1275	suggest	1269:1275	suggest that inulin-based EG is a potential fat substitute for developing healthier meat products, with better fatty acids composition and stable to chilled storage	1269:1432	CONCLUSION These results suggest that inulin-based EG is a potential fat substitute for developing healthier meat products, with better fatty acids composition and stable to chilled storage.
32648307	9	75	with	products	1358:1365	arg1	acids					1386:1390	better fatty acids	1373:1390	better fatty acids composition and stable to chilled storage	1373:1432	CONCLUSION These results suggest that inulin-based EG is a potential fat substitute for developing healthier meat products, with better fatty acids composition and stable to chilled storage.
32648307	6	76	theme	cohesive	883:890	arg1	samples					906:912	Softer, more elastic, cohesive and resilient samples	861:912	Softer, more elastic, cohesive and resilient samples with a higher intensity of lipid oxidation (P < 0.05)	861:966	Softer, more elastic, cohesive and resilient samples with a higher intensity of lipid oxidation (P < 0.05) were observed in EG added sausages.
32648307	5	77	theme	Microstructural	770:784	arg1	properties					786:795	Microstructural properties	770:795	Microstructural properties of sausages	770:807	Microstructural properties of sausages exhibited a denser network with the presence of EG.
32648307	1	78	theme	fat	204:206	arg1	gel					117:119	A high-fiber emulsion gel	95:119	A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil	95:180	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32648307	1	78	theme	fat	204:206	arg1	replacer					208:215	animal fat replacer	197:215	animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat	197:330	BACKGROUND A high-fiber emulsion gel (EG) containing inulin, soy protein isolate, and soybean oil was applied as animal fat replacer in reduced salt and fat Bologna sausage containing mechanically deboned chicken meat, pork meat, and pork back fat.
32799366	10	0	used	used	1479:1482	arg2	spectroscopy					1459:1470	Electron paramagnetic resonance spectroscopy	1427:1470	Electron paramagnetic resonance spectroscopy	1427:1470	Electron paramagnetic resonance spectroscopy can be used to analyze the irradiation history of dry products containing cellulose and sugar, such as the popular dog treat dried sweet potatoes, to ensure the products were irradiated within safe limits.
32799366	10	1	theme	resonance	1449:1457	arg1	spectroscopy					1459:1470	Electron paramagnetic resonance spectroscopy	1427:1470	Electron paramagnetic resonance spectroscopy	1427:1470	Electron paramagnetic resonance spectroscopy can be used to analyze the irradiation history of dry products containing cellulose and sugar, such as the popular dog treat dried sweet potatoes, to ensure the products were irradiated within safe limits.
32799366	6	2	theme	50	778:779	arg1	%					780:780	%	780:780	%	780:780	The sieving caused ca. 50% decrease in total signal intensity as compared to nonsieved samples due to loss of cellulose-based radicals.
32799366	3	3	contain	containing	406:415	arg2	sugars					431:436	sugars	431:436	sugars	431:436	These irradiation-specific radicals can be used to characterize the irradiation history of dry plant-based foods containing cellulose and sugars.
32799366	3	3	contain	containing	406:415	arg2	cellulose					417:425	cellulose	417:425	cellulose	417:425	These irradiation-specific radicals can be used to characterize the irradiation history of dry plant-based foods containing cellulose and sugars.
32799366	3	3	contain	containing	406:415	arg1	foods					400:404	dry plant-based foods	384:404	dry plant-based foods containing cellulose and sugars	384:436	These irradiation-specific radicals can be used to characterize the irradiation history of dry plant-based foods containing cellulose and sugars.
32799366	4	4	theme	preparation	580:590	arg1	method					592:597	sample preparation method	573:597	sample preparation method (grinding, sieving, and pelletizing)	573:634	The signal characteristics (intensity and peak shape) were evaluated at different sample locations (skin and flesh), as a function of sample preparation method (grinding, sieving, and pelletizing).
32799366	5	5	theme	signal	641:646	arg1	intensity					648:656	The signal intensity	637:656	The signal intensity	637:656	The signal intensity was quantified using a double integration method of the peaks based on the area under the curve.
32799366	7	6	theme	split	1022:1026	arg1	peak					1028:1031	split peak	1022:1031	split peak of dextrose radicals (337.4 mT)	1022:1063	The flesh of irradiated SP showed complex EPR spectra with multiple satellite peaks of cellulose radicals (333.5 and 338.8 mT) and split peak of dextrose radicals (337.4 mT); while skin spectra were distinctive of cellulose radicals.
32799366	1	7	theme	shelf	168:172	arg1	life					174:177	shelf life	168:177	shelf life	168:177	Dried sweet potatoes (SPs) are often irradiated for improved safety and shelf life.
32799366	8	8	theme	sample	1171:1176	arg1	composition					1178:1188	sample composition	1171:1188	sample composition	1171:1188	In this study, we demonstrated the effects of sample composition and preparation method on formation and analysis of irradiation-specific radicals based on EPR.
32799366	10	9	theme	irradiation	1499:1509	arg1	history					1511:1517	the irradiation history	1495:1517	the irradiation history of dry products containing cellulose and sugar, such as the popular dog	1495:1589	Electron paramagnetic resonance spectroscopy can be used to analyze the irradiation history of dry products containing cellulose and sugar, such as the popular dog treat dried sweet potatoes, to ensure the products were irradiated within safe limits.
32799366	5	10	theme	double	681:686	arg1	method					700:705	a double integration method	679:705	a double integration method of the peaks based on the area under the curve	679:752	The signal intensity was quantified using a double integration method of the peaks based on the area under the curve.
32799366	11	11	theme	radicals	1744:1751	arg1	formation					1710:1718	the formation	1706:1718	the formation of irradiation-specific radicals	1706:1751	This work demonstrates that the formation of irradiation-specific radicals is affected by the sample location (skin and flesh) and moisture content.
32799366	10	12	dep	analyze	1487:1493	arg1	treat					1591:1595	treat	1591:1595	treat	1591:1595	Electron paramagnetic resonance spectroscopy can be used to analyze the irradiation history of dry products containing cellulose and sugar, such as the popular dog treat dried sweet potatoes, to ensure the products were irradiated within safe limits.
32799366	7	13	theme	skin	1072:1075	arg1	spectra					1077:1083	skin spectra	1072:1083	skin spectra	1072:1083	The flesh of irradiated SP showed complex EPR spectra with multiple satellite peaks of cellulose radicals (333.5 and 338.8 mT) and split peak of dextrose radicals (337.4 mT); while skin spectra were distinctive of cellulose radicals.
32799366	8	14	from	effects	1160:1166	arg1	analysis					1230:1237	analysis	1230:1237	analysis of irradiation-specific radicals based on EPR	1230:1283	In this study, we demonstrated the effects of sample composition and preparation method on formation and analysis of irradiation-specific radicals based on EPR.
32799366	8	14	from	effects	1160:1166	arg1	formation					1216:1224	formation	1216:1224	formation	1216:1224	In this study, we demonstrated the effects of sample composition and preparation method on formation and analysis of irradiation-specific radicals based on EPR.
32799366	3	15	theme	irradiation-specific	299:318	arg1	radicals					320:327	These irradiation-specific radicals	293:327	These irradiation-specific radicals	293:327	These irradiation-specific radicals can be used to characterize the irradiation history of dry plant-based foods containing cellulose and sugars.
32799366	8	16	theme	method	1206:1211	arg1	effects					1160:1166	the effects	1156:1166	the effects of sample composition and preparation method on formation and analysis of irradiation-specific radicals based on EPR	1156:1283	In this study, we demonstrated the effects of sample composition and preparation method on formation and analysis of irradiation-specific radicals based on EPR.
32799366	10	17	theme	products	1526:1533	arg1	history					1511:1517	the irradiation history	1495:1517	the irradiation history of dry products containing cellulose and sugar, such as the popular dog	1495:1589	Electron paramagnetic resonance spectroscopy can be used to analyze the irradiation history of dry products containing cellulose and sugar, such as the popular dog treat dried sweet potatoes, to ensure the products were irradiated within safe limits.
32799366	2	18	theme	irradiation-derived	193:211	arg1	radicals					213:220	irradiation-derived radicals	193:220	irradiation-derived radicals	193:220	Formation of irradiation-derived radicals was analyzed using electron paramagnetic resonance (EPR) spectroscopy.
32799366	7	19	theme	multiple	950:957	arg1	peaks					969:973	multiple satellite peaks	950:973	multiple satellite peaks of cellulose radicals (333.5 and 338.8 mT) and split peak of dextrose radicals (337.4 mT)	950:1063	The flesh of irradiated SP showed complex EPR spectra with multiple satellite peaks of cellulose radicals (333.5 and 338.8 mT) and split peak of dextrose radicals (337.4 mT); while skin spectra were distinctive of cellulose radicals.
32799366	9	20	theme	irradiated	1397:1406	arg1	products					1417:1424	irradiated pet food products	1397:1424	irradiated pet food products	1397:1424	PRACTICAL APPLICATION: In the last decade or so, there have been health concerns related to the consumption of irradiated pet food products.
32799366	6	21	theme	radicals	881:888	arg1	loss					857:860	loss	857:860	loss of cellulose-based radicals	857:888	The sieving caused ca. 50% decrease in total signal intensity as compared to nonsieved samples due to loss of cellulose-based radicals.
32799366	7	22	theme	radicals	988:995	arg1	peaks					969:973	multiple satellite peaks	950:973	multiple satellite peaks of cellulose radicals (333.5 and 338.8 mT) and split peak of dextrose radicals (337.4 mT)	950:1063	The flesh of irradiated SP showed complex EPR spectra with multiple satellite peaks of cellulose radicals (333.5 and 338.8 mT) and split peak of dextrose radicals (337.4 mT); while skin spectra were distinctive of cellulose radicals.
32799366	2	23	theme	paramagnetic	250:261	arg1	EPR					274:276	EPR	274:276	EPR	274:276	Formation of irradiation-derived radicals was analyzed using electron paramagnetic resonance (EPR) spectroscopy.
32799366	2	23	theme	paramagnetic	250:261	arg1	resonance					263:271	electron paramagnetic resonance	241:271	electron paramagnetic resonance (EPR) spectroscopy	241:290	Formation of irradiation-derived radicals was analyzed using electron paramagnetic resonance (EPR) spectroscopy.
32799366	9	24	theme	food	1412:1415	arg1	products					1417:1424	irradiated pet food products	1397:1424	irradiated pet food products	1397:1424	PRACTICAL APPLICATION: In the last decade or so, there have been health concerns related to the consumption of irradiated pet food products.
32799366	4	25	theme	signal	443:448	arg1	intensity					467:475	intensity	467:475	intensity	467:475	The signal characteristics (intensity and peak shape) were evaluated at different sample locations (skin and flesh), as a function of sample preparation method (grinding, sieving, and pelletizing).
32799366	4	25	theme	signal	443:448	arg1	shape					486:490	peak shape	481:490	peak shape	481:490	The signal characteristics (intensity and peak shape) were evaluated at different sample locations (skin and flesh), as a function of sample preparation method (grinding, sieving, and pelletizing).
32799366	4	25	theme	signal	443:448	arg1	characteristics					450:464	The signal characteristics	439:464	The signal characteristics (intensity and peak shape)	439:491	The signal characteristics (intensity and peak shape) were evaluated at different sample locations (skin and flesh), as a function of sample preparation method (grinding, sieving, and pelletizing).
32799366	4	25	theme	signal	443:448	arg1	function					561:568	a function	559:568	a function of sample preparation method (grinding, sieving, and pelletizing)	559:634	The signal characteristics (intensity and peak shape) were evaluated at different sample locations (skin and flesh), as a function of sample preparation method (grinding, sieving, and pelletizing).
32799366	0	26	theme	sweet	57:61	arg1	potatoes					63:70	sweet potatoes	57:70	sweet potatoes	57:70	An analysis of cellulose- and dextrose-based radicals in sweet potatoes as irradiation markers.
32799366	7	27	theme	complex	925:931	arg1	spectra					937:943	complex EPR spectra	925:943	complex EPR spectra	925:943	The flesh of irradiated SP showed complex EPR spectra with multiple satellite peaks of cellulose radicals (333.5 and 338.8 mT) and split peak of dextrose radicals (337.4 mT); while skin spectra were distinctive of cellulose radicals.
32799366	8	28	theme	irradiation-specific	1242:1261	arg1	radicals					1263:1270	irradiation-specific radicals	1242:1270	irradiation-specific radicals based on EPR	1242:1283	In this study, we demonstrated the effects of sample composition and preparation method on formation and analysis of irradiation-specific radicals based on EPR.
32799366	7	29	theme	SP	915:916	arg1	flesh					895:899	The flesh	891:899	The flesh of irradiated SP	891:916	The flesh of irradiated SP showed complex EPR spectra with multiple satellite peaks of cellulose radicals (333.5 and 338.8 mT) and split peak of dextrose radicals (337.4 mT); while skin spectra were distinctive of cellulose radicals.
32799366	7	30	theme	radicals	1045:1052	arg1	peak					1028:1031	split peak	1022:1031	split peak of dextrose radicals (337.4 mT)	1022:1063	The flesh of irradiated SP showed complex EPR spectra with multiple satellite peaks of cellulose radicals (333.5 and 338.8 mT) and split peak of dextrose radicals (337.4 mT); while skin spectra were distinctive of cellulose radicals.
32799366	7	30	theme	radicals	1045:1052	arg1	mT					1014:1015	333.5 and 338.8 mT	998:1015	333.5 and 338.8 mT	998:1015	The flesh of irradiated SP showed complex EPR spectra with multiple satellite peaks of cellulose radicals (333.5 and 338.8 mT) and split peak of dextrose radicals (337.4 mT); while skin spectra were distinctive of cellulose radicals.
32799366	7	30	theme	radicals	1045:1052	arg1	radicals					988:995	cellulose radicals	978:995	cellulose radicals (333.5 and 338.8 mT)	978:1016	The flesh of irradiated SP showed complex EPR spectra with multiple satellite peaks of cellulose radicals (333.5 and 338.8 mT) and split peak of dextrose radicals (337.4 mT); while skin spectra were distinctive of cellulose radicals.
32799366	3	31	theme	irradiation	361:371	arg1	history					373:379	the irradiation history	357:379	the irradiation history of dry plant-based foods containing cellulose and sugars	357:436	These irradiation-specific radicals can be used to characterize the irradiation history of dry plant-based foods containing cellulose and sugars.
32799366	4	32	theme	sample	521:526	arg1	flesh					548:552	flesh	548:552	flesh	548:552	The signal characteristics (intensity and peak shape) were evaluated at different sample locations (skin and flesh), as a function of sample preparation method (grinding, sieving, and pelletizing).
32799366	4	32	theme	sample	521:526	arg1	skin					539:542	skin	539:542	skin	539:542	The signal characteristics (intensity and peak shape) were evaluated at different sample locations (skin and flesh), as a function of sample preparation method (grinding, sieving, and pelletizing).
32799366	4	32	theme	sample	521:526	arg1	locations					528:536	different sample locations	511:536	different sample locations (skin and flesh)	511:553	The signal characteristics (intensity and peak shape) were evaluated at different sample locations (skin and flesh), as a function of sample preparation method (grinding, sieving, and pelletizing).
32799366	3	33	used	used	336:339	arg2	radicals					320:327	These irradiation-specific radicals	293:327	These irradiation-specific radicals	293:327	These irradiation-specific radicals can be used to characterize the irradiation history of dry plant-based foods containing cellulose and sugars.
32799366	0	34	from	radicals	45:52	arg1	potatoes					63:70	sweet potatoes	57:70	sweet potatoes	57:70	An analysis of cellulose- and dextrose-based radicals in sweet potatoes as irradiation markers.
32799366	7	35	theme	cellulose	1105:1113	arg1	radicals					1115:1122	cellulose radicals	1105:1122	cellulose radicals	1105:1122	The flesh of irradiated SP showed complex EPR spectra with multiple satellite peaks of cellulose radicals (333.5 and 338.8 mT) and split peak of dextrose radicals (337.4 mT); while skin spectra were distinctive of cellulose radicals.
32799366	3	36	theme	plant-based	388:398	arg1	foods					400:404	dry plant-based foods	384:404	dry plant-based foods containing cellulose and sugars	384:436	These irradiation-specific radicals can be used to characterize the irradiation history of dry plant-based foods containing cellulose and sugars.
32799366	1	37	theme	sweet	102:106	arg1	SPs					118:120	SPs	118:120	SPs	118:120	Dried sweet potatoes (SPs) are often irradiated for improved safety and shelf life.
32799366	1	37	theme	sweet	102:106	arg1	potatoes					108:115	Dried sweet potatoes	96:115	Dried sweet potatoes (SPs)	96:121	Dried sweet potatoes (SPs) are often irradiated for improved safety and shelf life.
32799366	10	38	theme	popular	1579:1585	arg1	dog					1587:1589	the popular dog	1575:1589	the popular dog	1575:1589	Electron paramagnetic resonance spectroscopy can be used to analyze the irradiation history of dry products containing cellulose and sugar, such as the popular dog treat dried sweet potatoes, to ensure the products were irradiated within safe limits.
32799366	4	39	dep	locations	528:536	arg1	flesh					548:552	flesh	548:552	flesh	548:552	The signal characteristics (intensity and peak shape) were evaluated at different sample locations (skin and flesh), as a function of sample preparation method (grinding, sieving, and pelletizing).
32799366	4	39	dep	locations	528:536	arg1	skin					539:542	skin	539:542	skin	539:542	The signal characteristics (intensity and peak shape) were evaluated at different sample locations (skin and flesh), as a function of sample preparation method (grinding, sieving, and pelletizing).
32799366	4	39	dep	locations	528:536	arg1	locations					528:536	different sample locations	511:536	different sample locations (skin and flesh)	511:553	The signal characteristics (intensity and peak shape) were evaluated at different sample locations (skin and flesh), as a function of sample preparation method (grinding, sieving, and pelletizing).
32799366	10	40	theme	paramagnetic	1436:1447	arg1	resonance					1449:1457	Electron paramagnetic resonance	1427:1457	Electron paramagnetic resonance spectroscopy	1427:1470	Electron paramagnetic resonance spectroscopy can be used to analyze the irradiation history of dry products containing cellulose and sugar, such as the popular dog treat dried sweet potatoes, to ensure the products were irradiated within safe limits.
32799366	0	41	theme	radicals	45:52	arg1	analysis					3:10	An analysis	0:10	An analysis of cellulose- and dextrose-based radicals in sweet potatoes as irradiation markers.	0:94	An analysis of cellulose- and dextrose-based radicals in sweet potatoes as irradiation markers.
32799366	6	42	theme	total	794:798	arg1	intensity					807:815	total signal intensity	794:815	total signal intensity	794:815	The sieving caused ca. 50% decrease in total signal intensity as compared to nonsieved samples due to loss of cellulose-based radicals.
32799366	10	43	theme	sweet	1603:1607	arg1	potatoes					1609:1616	dried sweet potatoes	1597:1616	dried sweet potatoes	1597:1616	Electron paramagnetic resonance spectroscopy can be used to analyze the irradiation history of dry products containing cellulose and sugar, such as the popular dog treat dried sweet potatoes, to ensure the products were irradiated within safe limits.
32799366	4	44	dep	characteristics	450:464	arg1	intensity					467:475	intensity	467:475	intensity	467:475	The signal characteristics (intensity and peak shape) were evaluated at different sample locations (skin and flesh), as a function of sample preparation method (grinding, sieving, and pelletizing).
32799366	4	44	dep	characteristics	450:464	arg1	shape					486:490	peak shape	481:490	peak shape	481:490	The signal characteristics (intensity and peak shape) were evaluated at different sample locations (skin and flesh), as a function of sample preparation method (grinding, sieving, and pelletizing).
32799366	4	44	dep	characteristics	450:464	arg1	characteristics					450:464	The signal characteristics	439:464	The signal characteristics (intensity and peak shape)	439:491	The signal characteristics (intensity and peak shape) were evaluated at different sample locations (skin and flesh), as a function of sample preparation method (grinding, sieving, and pelletizing).
32799366	10	45	contain	containing	1535:1544	arg2	sugar					1560:1564	sugar	1560:1564	sugar	1560:1564	Electron paramagnetic resonance spectroscopy can be used to analyze the irradiation history of dry products containing cellulose and sugar, such as the popular dog treat dried sweet potatoes, to ensure the products were irradiated within safe limits.
32799366	10	45	contain	containing	1535:1544	arg2	cellulose					1546:1554	cellulose	1546:1554	cellulose	1546:1554	Electron paramagnetic resonance spectroscopy can be used to analyze the irradiation history of dry products containing cellulose and sugar, such as the popular dog treat dried sweet potatoes, to ensure the products were irradiated within safe limits.
32799366	10	45	contain	containing	1535:1544	arg1	dog					1587:1589	the popular dog	1575:1589	the popular dog	1575:1589	Electron paramagnetic resonance spectroscopy can be used to analyze the irradiation history of dry products containing cellulose and sugar, such as the popular dog treat dried sweet potatoes, to ensure the products were irradiated within safe limits.
32799366	10	45	contain	containing	1535:1544	arg1	products					1526:1533	dry products	1522:1533	dry products	1522:1533	Electron paramagnetic resonance spectroscopy can be used to analyze the irradiation history of dry products containing cellulose and sugar, such as the popular dog treat dried sweet potatoes, to ensure the products were irradiated within safe limits.
32799366	9	46	from	health	1351:1356	arg1	decade					1321:1326	the last decade	1312:1326	the last decade	1312:1326	PRACTICAL APPLICATION: In the last decade or so, there have been health concerns related to the consumption of irradiated pet food products.
32799366	4	47	theme	method	592:597	arg1	function					561:568	a function	559:568	a function of sample preparation method (grinding, sieving, and pelletizing)	559:634	The signal characteristics (intensity and peak shape) were evaluated at different sample locations (skin and flesh), as a function of sample preparation method (grinding, sieving, and pelletizing).
32799366	4	47	theme	method	592:597	arg1	characteristics					450:464	The signal characteristics	439:464	The signal characteristics (intensity and peak shape)	439:491	The signal characteristics (intensity and peak shape) were evaluated at different sample locations (skin and flesh), as a function of sample preparation method (grinding, sieving, and pelletizing).
32799366	9	48	theme	last	1316:1319	arg1	decade					1321:1326	the last decade	1312:1326	the last decade	1312:1326	PRACTICAL APPLICATION: In the last decade or so, there have been health concerns related to the consumption of irradiated pet food products.
32799366	6	49	theme	cellulose-based	865:879	arg1	radicals					881:888	cellulose-based radicals	865:888	cellulose-based radicals	865:888	The sieving caused ca. 50% decrease in total signal intensity as compared to nonsieved samples due to loss of cellulose-based radicals.
32799366	7	50	theme	dextrose	1036:1043	arg1	radicals					1045:1052	dextrose radicals	1036:1052	dextrose radicals (337.4 mT)	1036:1063	The flesh of irradiated SP showed complex EPR spectra with multiple satellite peaks of cellulose radicals (333.5 and 338.8 mT) and split peak of dextrose radicals (337.4 mT); while skin spectra were distinctive of cellulose radicals.
32799366	7	50	theme	dextrose	1036:1043	arg1	mT					1061:1062	337.4 mT	1055:1062	337.4 mT	1055:1062	The flesh of irradiated SP showed complex EPR spectra with multiple satellite peaks of cellulose radicals (333.5 and 338.8 mT) and split peak of dextrose radicals (337.4 mT); while skin spectra were distinctive of cellulose radicals.
32799366	10	51	theme	safe	1665:1668	arg1	limits					1670:1675	safe limits	1665:1675	safe limits	1665:1675	Electron paramagnetic resonance spectroscopy can be used to analyze the irradiation history of dry products containing cellulose and sugar, such as the popular dog treat dried sweet potatoes, to ensure the products were irradiated within safe limits.
32799366	4	52	theme	sample	573:578	arg1	method					592:597	sample preparation method	573:597	sample preparation method (grinding, sieving, and pelletizing)	573:634	The signal characteristics (intensity and peak shape) were evaluated at different sample locations (skin and flesh), as a function of sample preparation method (grinding, sieving, and pelletizing).
32799366	7	53	theme	peak	1028:1031	arg1	peaks					969:973	multiple satellite peaks	950:973	multiple satellite peaks of cellulose radicals (333.5 and 338.8 mT) and split peak of dextrose radicals (337.4 mT)	950:1063	The flesh of irradiated SP showed complex EPR spectra with multiple satellite peaks of cellulose radicals (333.5 and 338.8 mT) and split peak of dextrose radicals (337.4 mT); while skin spectra were distinctive of cellulose radicals.
32799366	1	54	theme	improved	148:155	arg1	safety					157:162	improved safety	148:162	improved safety	148:162	Dried sweet potatoes (SPs) are often irradiated for improved safety and shelf life.
32799366	6	55	theme	nonsieved	832:840	arg1	samples					842:848	nonsieved samples	832:848	nonsieved samples	832:848	The sieving caused ca. 50% decrease in total signal intensity as compared to nonsieved samples due to loss of cellulose-based radicals.
32799366	10	56	theme	dry	1522:1524	arg1	dog					1587:1589	the popular dog	1575:1589	the popular dog	1575:1589	Electron paramagnetic resonance spectroscopy can be used to analyze the irradiation history of dry products containing cellulose and sugar, such as the popular dog treat dried sweet potatoes, to ensure the products were irradiated within safe limits.
32799366	10	56	theme	dry	1522:1524	arg1	products					1526:1533	dry products	1522:1533	dry products	1522:1533	Electron paramagnetic resonance spectroscopy can be used to analyze the irradiation history of dry products containing cellulose and sugar, such as the popular dog treat dried sweet potatoes, to ensure the products were irradiated within safe limits.
32799366	6	57	from	decrease	782:789	arg1	intensity					807:815	total signal intensity	794:815	total signal intensity	794:815	The sieving caused ca. 50% decrease in total signal intensity as compared to nonsieved samples due to loss of cellulose-based radicals.
32799366	11	58	theme	sample	1772:1777	arg1	location					1779:1786	the sample location	1768:1786	the sample location (skin and flesh)	1768:1803	This work demonstrates that the formation of irradiation-specific radicals is affected by the sample location (skin and flesh) and moisture content.
32799366	5	59	theme	integration	688:698	arg1	method					700:705	a double integration method	679:705	a double integration method of the peaks based on the area under the curve	679:752	The signal intensity was quantified using a double integration method of the peaks based on the area under the curve.
32799366	0	60	from	potatoes	63:70	arg1	analysis					3:10	An analysis	0:10	An analysis of cellulose- and dextrose-based radicals in sweet potatoes as irradiation markers.	0:94	An analysis of cellulose- and dextrose-based radicals in sweet potatoes as irradiation markers.
32799366	8	61	theme	composition	1178:1188	arg1	effects					1160:1166	the effects	1156:1166	the effects of sample composition and preparation method on formation and analysis of irradiation-specific radicals based on EPR	1156:1283	In this study, we demonstrated the effects of sample composition and preparation method on formation and analysis of irradiation-specific radicals based on EPR.
32799366	2	62	theme	radicals	213:220	arg1	Formation					180:188	Formation	180:188	Formation of irradiation-derived radicals	180:220	Formation of irradiation-derived radicals was analyzed using electron paramagnetic resonance (EPR) spectroscopy.
32799366	8	63	theme	preparation	1194:1204	arg1	method					1206:1211	preparation method	1194:1211	preparation method	1194:1211	In this study, we demonstrated the effects of sample composition and preparation method on formation and analysis of irradiation-specific radicals based on EPR.
32799366	11	64	theme	irradiation-specific	1723:1742	arg1	radicals					1744:1751	irradiation-specific radicals	1723:1751	irradiation-specific radicals	1723:1751	This work demonstrates that the formation of irradiation-specific radicals is affected by the sample location (skin and flesh) and moisture content.
32799366	7	65	theme	cellulose	978:986	arg1	mT					1014:1015	333.5 and 338.8 mT	998:1015	333.5 and 338.8 mT	998:1015	The flesh of irradiated SP showed complex EPR spectra with multiple satellite peaks of cellulose radicals (333.5 and 338.8 mT) and split peak of dextrose radicals (337.4 mT); while skin spectra were distinctive of cellulose radicals.
32799366	7	65	theme	cellulose	978:986	arg1	radicals					988:995	cellulose radicals	978:995	cellulose radicals (333.5 and 338.8 mT)	978:1016	The flesh of irradiated SP showed complex EPR spectra with multiple satellite peaks of cellulose radicals (333.5 and 338.8 mT) and split peak of dextrose radicals (337.4 mT); while skin spectra were distinctive of cellulose radicals.
32799366	2	66	theme	resonance	263:271	arg1	spectroscopy					279:290	electron paramagnetic resonance (EPR) spectroscopy	241:290	electron paramagnetic resonance (EPR) spectroscopy	241:290	Formation of irradiation-derived radicals was analyzed using electron paramagnetic resonance (EPR) spectroscopy.
32799366	7	67	theme	satellite	959:967	arg1	peaks					969:973	multiple satellite peaks	950:973	multiple satellite peaks of cellulose radicals (333.5 and 338.8 mT) and split peak of dextrose radicals (337.4 mT)	950:1063	The flesh of irradiated SP showed complex EPR spectra with multiple satellite peaks of cellulose radicals (333.5 and 338.8 mT) and split peak of dextrose radicals (337.4 mT); while skin spectra were distinctive of cellulose radicals.
32799366	9	68	theme	pet	1408:1410	arg1	products					1417:1424	irradiated pet food products	1397:1424	irradiated pet food products	1397:1424	PRACTICAL APPLICATION: In the last decade or so, there have been health concerns related to the consumption of irradiated pet food products.
32799366	2	69	link	irradiation-derived	193:211	arg1	radicals					213:220	irradiation-derived radicals	193:220	irradiation-derived radicals	193:220	Formation of irradiation-derived radicals was analyzed using electron paramagnetic resonance (EPR) spectroscopy.
32799366	4	70	dep	method	592:597	arg1	sieving					610:616	sieving	610:616	sieving	610:616	The signal characteristics (intensity and peak shape) were evaluated at different sample locations (skin and flesh), as a function of sample preparation method (grinding, sieving, and pelletizing).
32799366	4	70	dep	method	592:597	arg1	pelletizing					623:633	pelletizing	623:633	pelletizing	623:633	The signal characteristics (intensity and peak shape) were evaluated at different sample locations (skin and flesh), as a function of sample preparation method (grinding, sieving, and pelletizing).
32799366	4	70	dep	method	592:597	arg1	grinding					600:607	grinding	600:607	grinding	600:607	The signal characteristics (intensity and peak shape) were evaluated at different sample locations (skin and flesh), as a function of sample preparation method (grinding, sieving, and pelletizing).
32799366	2	71	theme	electron	241:248	arg1	EPR					274:276	EPR	274:276	EPR	274:276	Formation of irradiation-derived radicals was analyzed using electron paramagnetic resonance (EPR) spectroscopy.
32799366	2	71	theme	electron	241:248	arg1	resonance					263:271	electron paramagnetic resonance	241:271	electron paramagnetic resonance (EPR) spectroscopy	241:290	Formation of irradiation-derived radicals was analyzed using electron paramagnetic resonance (EPR) spectroscopy.
32799366	9	72	theme	products	1417:1424	arg1	consumption					1382:1392	the consumption	1378:1392	the consumption of irradiated pet food products	1378:1424	PRACTICAL APPLICATION: In the last decade or so, there have been health concerns related to the consumption of irradiated pet food products.
32799366	4	73	theme	different	511:519	arg1	flesh					548:552	flesh	548:552	flesh	548:552	The signal characteristics (intensity and peak shape) were evaluated at different sample locations (skin and flesh), as a function of sample preparation method (grinding, sieving, and pelletizing).
32799366	4	73	theme	different	511:519	arg1	skin					539:542	skin	539:542	skin	539:542	The signal characteristics (intensity and peak shape) were evaluated at different sample locations (skin and flesh), as a function of sample preparation method (grinding, sieving, and pelletizing).
32799366	4	73	theme	different	511:519	arg1	locations					528:536	different sample locations	511:536	different sample locations (skin and flesh)	511:553	The signal characteristics (intensity and peak shape) were evaluated at different sample locations (skin and flesh), as a function of sample preparation method (grinding, sieving, and pelletizing).
32799366	0	74	theme	irradiation	75:85	arg1	markers					87:93	irradiation markers	75:93	irradiation markers	75:93	An analysis of cellulose- and dextrose-based radicals in sweet potatoes as irradiation markers.
32799366	7	75	theme	EPR	933:935	arg1	spectra					937:943	complex EPR spectra	925:943	complex EPR spectra	925:943	The flesh of irradiated SP showed complex EPR spectra with multiple satellite peaks of cellulose radicals (333.5 and 338.8 mT) and split peak of dextrose radicals (337.4 mT); while skin spectra were distinctive of cellulose radicals.
32799366	0	76	from	analysis	3:10	arg1	potatoes					63:70	sweet potatoes	57:70	sweet potatoes	57:70	An analysis of cellulose- and dextrose-based radicals in sweet potatoes as irradiation markers.
32799366	7	77	theme	radicals	1115:1122	arg1	distinctive					1090:1100	distinctive	1090:1100	distinctive	1090:1100	The flesh of irradiated SP showed complex EPR spectra with multiple satellite peaks of cellulose radicals (333.5 and 338.8 mT) and split peak of dextrose radicals (337.4 mT); while skin spectra were distinctive of cellulose radicals.
32799366	9	78	dep	APPLICATION	1296:1306	arg1	health					1351:1356	health	1351:1356	health	1351:1356	PRACTICAL APPLICATION: In the last decade or so, there have been health concerns related to the consumption of irradiated pet food products.
32799366	8	79	theme	radicals	1263:1270	arg1	analysis					1230:1237	analysis	1230:1237	analysis of irradiation-specific radicals based on EPR	1230:1283	In this study, we demonstrated the effects of sample composition and preparation method on formation and analysis of irradiation-specific radicals based on EPR.
32799366	8	79	theme	radicals	1263:1270	arg1	formation					1216:1224	formation	1216:1224	formation	1216:1224	In this study, we demonstrated the effects of sample composition and preparation method on formation and analysis of irradiation-specific radicals based on EPR.
32799366	5	80	theme	peaks	714:718	arg1	method					700:705	a double integration method	679:705	a double integration method of the peaks based on the area under the curve	679:752	The signal intensity was quantified using a double integration method of the peaks based on the area under the curve.
32799366	7	81	theme	irradiated	904:913	arg1	SP					915:916	irradiated SP	904:916	irradiated SP	904:916	The flesh of irradiated SP showed complex EPR spectra with multiple satellite peaks of cellulose radicals (333.5 and 338.8 mT) and split peak of dextrose radicals (337.4 mT); while skin spectra were distinctive of cellulose radicals.
32799366	11	82	theme	moisture	1809:1816	arg1	content					1818:1824	moisture content	1809:1824	moisture content	1809:1824	This work demonstrates that the formation of irradiation-specific radicals is affected by the sample location (skin and flesh) and moisture content.
32799366	11	83	dep	location	1779:1786	arg1	flesh					1798:1802	flesh	1798:1802	flesh	1798:1802	This work demonstrates that the formation of irradiation-specific radicals is affected by the sample location (skin and flesh) and moisture content.
32799366	11	83	dep	location	1779:1786	arg1	skin					1789:1792	skin	1789:1792	skin	1789:1792	This work demonstrates that the formation of irradiation-specific radicals is affected by the sample location (skin and flesh) and moisture content.
32799366	3	84	theme	foods	400:404	arg1	history					373:379	the irradiation history	357:379	the irradiation history of dry plant-based foods containing cellulose and sugars	357:436	These irradiation-specific radicals can be used to characterize the irradiation history of dry plant-based foods containing cellulose and sugars.
32799366	0	85	theme	cellulose-	15:24	arg1	radicals					45:52	cellulose- and dextrose-based radicals	15:52	cellulose- and dextrose-based radicals in sweet potatoes as irradiation markers	15:93	An analysis of cellulose- and dextrose-based radicals in sweet potatoes as irradiation markers.
32799366	1	86	theme	Dried	96:100	arg1	SPs					118:120	SPs	118:120	SPs	118:120	Dried sweet potatoes (SPs) are often irradiated for improved safety and shelf life.
32799366	1	86	theme	Dried	96:100	arg1	potatoes					108:115	Dried sweet potatoes	96:115	Dried sweet potatoes (SPs)	96:121	Dried sweet potatoes (SPs) are often irradiated for improved safety and shelf life.
32799366	3	87	theme	dry	384:386	arg1	foods					400:404	dry plant-based foods	384:404	dry plant-based foods containing cellulose and sugars	384:436	These irradiation-specific radicals can be used to characterize the irradiation history of dry plant-based foods containing cellulose and sugars.
32799366	0	88	theme	dextrose-based	30:43	arg1	radicals					45:52	cellulose- and dextrose-based radicals	15:52	cellulose- and dextrose-based radicals in sweet potatoes as irradiation markers	15:93	An analysis of cellulose- and dextrose-based radicals in sweet potatoes as irradiation markers.
32799366	9	89	theme	PRACTICAL	1286:1294	arg1	APPLICATION					1296:1306	PRACTICAL APPLICATION	1286:1306	PRACTICAL APPLICATION: In the last decade or so, there have been health	1286:1356	PRACTICAL APPLICATION: In the last decade or so, there have been health concerns related to the consumption of irradiated pet food products.
32799366	6	90	theme	signal	800:805	arg1	intensity					807:815	total signal intensity	794:815	total signal intensity	794:815	The sieving caused ca. 50% decrease in total signal intensity as compared to nonsieved samples due to loss of cellulose-based radicals.
32799366	10	91	theme	Electron	1427:1434	arg1	resonance					1449:1457	Electron paramagnetic resonance	1427:1457	Electron paramagnetic resonance spectroscopy	1427:1470	Electron paramagnetic resonance spectroscopy can be used to analyze the irradiation history of dry products containing cellulose and sugar, such as the popular dog treat dried sweet potatoes, to ensure the products were irradiated within safe limits.
32799366	4	92	theme	peak	481:484	arg1	shape					486:490	peak shape	481:490	peak shape	481:490	The signal characteristics (intensity and peak shape) were evaluated at different sample locations (skin and flesh), as a function of sample preparation method (grinding, sieving, and pelletizing).
32799366	4	92	theme	peak	481:484	arg1	characteristics					450:464	The signal characteristics	439:464	The signal characteristics (intensity and peak shape)	439:491	The signal characteristics (intensity and peak shape) were evaluated at different sample locations (skin and flesh), as a function of sample preparation method (grinding, sieving, and pelletizing).
32799366	10	93	theme	dried	1597:1601	arg1	potatoes					1609:1616	dried sweet potatoes	1597:1616	dried sweet potatoes	1597:1616	Electron paramagnetic resonance spectroscopy can be used to analyze the irradiation history of dry products containing cellulose and sugar, such as the popular dog treat dried sweet potatoes, to ensure the products were irradiated within safe limits.
32799366	6	94	theme	%	780:780	arg1	decrease					782:789	50% decrease	778:789	50% decrease in total signal intensity	778:815	The sieving caused ca. 50% decrease in total signal intensity as compared to nonsieved samples due to loss of cellulose-based radicals.
33352218	11	0	from	changes	1839:1845	arg1	mice					1888:1891	colitis-induced mice	1872:1891	colitis-induced mice	1872:1891	These results provide evidence supporting protective effects of αT and γT on intestinal barrier function and that γTmT caused favorable changes of the gut microbiota in colitis-induced mice.
33352218	6	1	theme	electrical	1077:1086	arg1	resistance					1088:1097	trans-epithelial electrical resistance	1060:1097	trans-epithelial electrical resistance	1060:1097	Consistently, αT and γT mitigated TNF-α/IFN-γ-induced impairment of trans-epithelial electrical resistance in human intestinal epithelial Caco-2 cell monolayer.
33352218	3	2	theme	γ-tocopherol-rich	474:490	arg1	γTmT					505:508	γTmT	505:508	γTmT	505:508	The objective of this study is to examine the impact of α-tocopherol (αT) and γ-tocopherol-rich tocopherols (γTmT) on gut inflammation, barrier integrity and microbiota in dextran sulfate sodium (DSS)-induced colitis in mice.
33352218	3	2	theme	γ-tocopherol-rich	474:490	arg1	tocopherols					492:502	γ-tocopherol-rich tocopherols	474:502	γ-tocopherol-rich tocopherols (γTmT)	474:509	The objective of this study is to examine the impact of α-tocopherol (αT) and γ-tocopherol-rich tocopherols (γTmT) on gut inflammation, barrier integrity and microbiota in dextran sulfate sodium (DSS)-induced colitis in mice.
33352218	3	3	theme	gut	514:516	arg1	inflammation					518:529	gut inflammation	514:529	gut inflammation	514:529	The objective of this study is to examine the impact of α-tocopherol (αT) and γ-tocopherol-rich tocopherols (γTmT) on gut inflammation, barrier integrity and microbiota in dextran sulfate sodium (DSS)-induced colitis in mice.
33352218	5	4	theme	colitis-caused	827:840	arg1	elevation					842:850	colitis-caused elevation	827:850	colitis-caused elevation of LPS-binding protein in the plasma, a surrogate marker of intestinal barrier dysfunction	827:941	These vitamin E forms inhibited colitis-induced loss of the tight junction protein occludin, and attenuated colitis-caused elevation of LPS-binding protein in the plasma, a surrogate marker of intestinal barrier dysfunction, suggesting protection of gut barrier integrity.
33352218	2	5	theme	vitamin	303:309	arg1	E					311:311	vitamin E	303:311	vitamin E	303:311	Specific forms of vitamin E have been shown to attenuate colitis, but the mechanisms are not fully understood.
33352218	5	6	theme	junction	785:792	arg1	occludin					802:809	the tight junction protein occludin	775:809	the tight junction protein occludin	775:809	These vitamin E forms inhibited colitis-induced loss of the tight junction protein occludin, and attenuated colitis-caused elevation of LPS-binding protein in the plasma, a surrogate marker of intestinal barrier dysfunction, suggesting protection of gut barrier integrity.
33352218	5	7	theme	occludin	802:809	arg1	loss					767:770	colitis-induced loss	751:770	colitis-induced loss of the tight junction protein occludin	751:809	These vitamin E forms inhibited colitis-induced loss of the tight junction protein occludin, and attenuated colitis-caused elevation of LPS-binding protein in the plasma, a surrogate marker of intestinal barrier dysfunction, suggesting protection of gut barrier integrity.
33352218	8	8	theme	producing	1474:1482	arg1	bacteria					1484:1491	butyrate producing bacteria	1465:1491	butyrate producing bacteria	1465:1491	In colitis-induced mice, γTmT but not αT separated gut microbial composition from controls, and attenuated DSS-caused depletion of Roseburia, which contains butyrate producing bacteria and is decreased in IBD patients.
33352218	11	9	theme	γT	1774:1775	arg1	effects					1756:1762	protective effects	1745:1762	protective effects of αT and γT on intestinal barrier function	1745:1806	These results provide evidence supporting protective effects of αT and γT on intestinal barrier function and that γTmT caused favorable changes of the gut microbiota in colitis-induced mice.
33352218	3	10	from	integrity	540:548	arg1	colitis					605:611	dextran sulfate sodium (DSS)-induced colitis	568:611	dextran sulfate sodium (DSS)-induced colitis in mice	568:619	The objective of this study is to examine the impact of α-tocopherol (αT) and γ-tocopherol-rich tocopherols (γTmT) on gut inflammation, barrier integrity and microbiota in dextran sulfate sodium (DSS)-induced colitis in mice.
33352218	11	11	theme	αT	1767:1768	arg1	effects					1756:1762	protective effects	1745:1762	protective effects of αT and γT on intestinal barrier function	1745:1806	These results provide evidence supporting protective effects of αT and γT on intestinal barrier function and that γTmT caused favorable changes of the gut microbiota in colitis-induced mice.
33352218	9	12	theme	Canonical	1527:1535	arg1	analysis					1552:1559	Canonical correspondence analysis	1527:1559	Canonical correspondence analysis	1527:1559	Canonical correspondence analysis also supports that γTmT favorably altered gut microbial community.
33352218	11	13	from	effects	1756:1762	arg1	function					1799:1806	intestinal barrier function	1780:1806	intestinal barrier function	1780:1806	These results provide evidence supporting protective effects of αT and γT on intestinal barrier function and that γTmT caused favorable changes of the gut microbiota in colitis-induced mice.
33352218	0	14	theme	barrier	75:81	arg1	function					83:90	intestinal barrier function	64:90	intestinal barrier function	64:90	Vitamin E alpha- and gamma-tocopherol mitigate colitis, protect intestinal barrier function and modulate the gut microbiota in mice.
33352218	5	15	theme	protein	867:873	arg1	elevation					842:850	colitis-caused elevation	827:850	colitis-caused elevation of LPS-binding protein in the plasma, a surrogate marker of intestinal barrier dysfunction	827:941	These vitamin E forms inhibited colitis-induced loss of the tight junction protein occludin, and attenuated colitis-caused elevation of LPS-binding protein in the plasma, a surrogate marker of intestinal barrier dysfunction, suggesting protection of gut barrier integrity.
33352218	8	16	theme	IBD	1513:1515	arg1	patients					1517:1524	IBD patients	1513:1524	IBD patients	1513:1524	In colitis-induced mice, γTmT but not αT separated gut microbial composition from controls, and attenuated DSS-caused depletion of Roseburia, which contains butyrate producing bacteria and is decreased in IBD patients.
33352218	4	17	theme	IL-6	713:716	arg1	bleeding					677:684	bleeding	677:684	bleeding	677:684	We observe that αT and γTmT mitigated DSS-caused fecal bleeding, diarrhea and elevation of IL-6.
33352218	11	18	theme	intestinal	1780:1789	arg1	function					1799:1806	intestinal barrier function	1780:1806	intestinal barrier function	1780:1806	These results provide evidence supporting protective effects of αT and γT on intestinal barrier function and that γTmT caused favorable changes of the gut microbiota in colitis-induced mice.
33352218	5	19	from	elevation	842:850	arg1	plasma					882:887	the plasma	878:887	the plasma	878:887	These vitamin E forms inhibited colitis-induced loss of the tight junction protein occludin, and attenuated colitis-caused elevation of LPS-binding protein in the plasma, a surrogate marker of intestinal barrier dysfunction, suggesting protection of gut barrier integrity.
33352218	5	20	theme	barrier	923:929	arg1	dysfunction					931:941	intestinal barrier dysfunction	912:941	intestinal barrier dysfunction	912:941	These vitamin E forms inhibited colitis-induced loss of the tight junction protein occludin, and attenuated colitis-caused elevation of LPS-binding protein in the plasma, a surrogate marker of intestinal barrier dysfunction, suggesting protection of gut barrier integrity.
33352218	7	21	theme	DNA	1193:1195	arg1	sequencing					1173:1182	16S rRNA gene sequencing	1159:1182	16S rRNA gene sequencing of fecal DNA	1159:1195	Using 16S rRNA gene sequencing of fecal DNA, we observe that DSS reduced gut microbial evenness and separated microbial composition from healthy controls.
33352218	5	22	from	plasma	882:887	arg1	elevation					842:850	colitis-caused elevation	827:850	colitis-caused elevation of LPS-binding protein in the plasma, a surrogate marker of intestinal barrier dysfunction	827:941	These vitamin E forms inhibited colitis-induced loss of the tight junction protein occludin, and attenuated colitis-caused elevation of LPS-binding protein in the plasma, a surrogate marker of intestinal barrier dysfunction, suggesting protection of gut barrier integrity.
33352218	11	23	theme	microbiota	1858:1867	arg1	changes					1839:1845	favorable changes	1829:1845	favorable changes of the gut microbiota in colitis-induced mice	1829:1891	These results provide evidence supporting protective effects of αT and γT on intestinal barrier function and that γTmT caused favorable changes of the gut microbiota in colitis-induced mice.
33352218	8	24	theme	microbial	1363:1371	arg1	composition					1373:1383	gut microbial composition	1359:1383	gut microbial composition from controls	1359:1397	In colitis-induced mice, γTmT but not αT separated gut microbial composition from controls, and attenuated DSS-caused depletion of Roseburia, which contains butyrate producing bacteria and is decreased in IBD patients.
33352218	0	25	theme	Vitamin	0:6	arg1	alpha-					10:15	Vitamin E alpha-	0:15	Vitamin E alpha-	0:15	Vitamin E alpha- and gamma-tocopherol mitigate colitis, protect intestinal barrier function and modulate the gut microbiota in mice.
33352218	5	26	theme	surrogate	892:900	arg1	marker					902:907	a surrogate marker	890:907	a surrogate marker of intestinal barrier dysfunction	890:941	These vitamin E forms inhibited colitis-induced loss of the tight junction protein occludin, and attenuated colitis-caused elevation of LPS-binding protein in the plasma, a surrogate marker of intestinal barrier dysfunction, suggesting protection of gut barrier integrity.
33352218	5	26	theme	surrogate	892:900	arg1	protein					867:873	LPS-binding protein	855:873	LPS-binding protein in the plasma	855:887	These vitamin E forms inhibited colitis-induced loss of the tight junction protein occludin, and attenuated colitis-caused elevation of LPS-binding protein in the plasma, a surrogate marker of intestinal barrier dysfunction, suggesting protection of gut barrier integrity.
33352218	3	27	from	microbiota	554:563	arg1	colitis					605:611	dextran sulfate sodium (DSS)-induced colitis	568:611	dextran sulfate sodium (DSS)-induced colitis in mice	568:619	The objective of this study is to examine the impact of α-tocopherol (αT) and γ-tocopherol-rich tocopherols (γTmT) on gut inflammation, barrier integrity and microbiota in dextran sulfate sodium (DSS)-induced colitis in mice.
33352218	7	28	theme	gene	1168:1171	arg1	sequencing					1173:1182	16S rRNA gene sequencing	1159:1182	16S rRNA gene sequencing of fecal DNA	1159:1195	Using 16S rRNA gene sequencing of fecal DNA, we observe that DSS reduced gut microbial evenness and separated microbial composition from healthy controls.
33352218	9	29	theme	microbial	1607:1615	arg1	community					1617:1625	gut microbial community	1603:1625	gut microbial community	1603:1625	Canonical correspondence analysis also supports that γTmT favorably altered gut microbial community.
33352218	10	30	theme	healthy	1686:1692	arg1	animals					1694:1700	healthy animals	1686:1700	healthy animals	1686:1700	In contrast, neither αT nor γTmT affected gut microbes in healthy animals.
33352218	5	31	theme	E	733:733	arg1	forms					735:739	These vitamin E forms	719:739	These vitamin E forms	719:739	These vitamin E forms inhibited colitis-induced loss of the tight junction protein occludin, and attenuated colitis-caused elevation of LPS-binding protein in the plasma, a surrogate marker of intestinal barrier dysfunction, suggesting protection of gut barrier integrity.
33352218	7	32	theme	16S	1159:1161	arg1	sequencing					1173:1182	16S rRNA gene sequencing	1159:1182	16S rRNA gene sequencing of fecal DNA	1159:1195	Using 16S rRNA gene sequencing of fecal DNA, we observe that DSS reduced gut microbial evenness and separated microbial composition from healthy controls.
33352218	5	33	theme	integrity	981:989	arg1	protection					955:964	protection	955:964	protection of gut barrier integrity	955:989	These vitamin E forms inhibited colitis-induced loss of the tight junction protein occludin, and attenuated colitis-caused elevation of LPS-binding protein in the plasma, a surrogate marker of intestinal barrier dysfunction, suggesting protection of gut barrier integrity.
33352218	7	34	theme	microbial	1230:1238	arg1	evenness					1240:1247	gut microbial evenness	1226:1247	gut microbial evenness	1226:1247	Using 16S rRNA gene sequencing of fecal DNA, we observe that DSS reduced gut microbial evenness and separated microbial composition from healthy controls.
33352218	3	35	theme	-induced	596:603	arg1	colitis					605:611	dextran sulfate sodium (DSS)-induced colitis	568:611	dextran sulfate sodium (DSS)-induced colitis in mice	568:619	The objective of this study is to examine the impact of α-tocopherol (αT) and γ-tocopherol-rich tocopherols (γTmT) on gut inflammation, barrier integrity and microbiota in dextran sulfate sodium (DSS)-induced colitis in mice.
33352218	11	36	theme	colitis-induced	1872:1886	arg1	mice					1888:1891	colitis-induced mice	1872:1891	colitis-induced mice	1872:1891	These results provide evidence supporting protective effects of αT and γT on intestinal barrier function and that γTmT caused favorable changes of the gut microbiota in colitis-induced mice.
33352218	6	37	theme	epithelial	1119:1128	arg1	monolayer					1142:1150	human intestinal epithelial Caco-2 cell monolayer	1102:1150	human intestinal epithelial Caco-2 cell monolayer	1102:1150	Consistently, αT and γT mitigated TNF-α/IFN-γ-induced impairment of trans-epithelial electrical resistance in human intestinal epithelial Caco-2 cell monolayer.
33352218	3	38	theme	α-tocopherol	452:463	arg1	impact					442:447	the impact	438:447	the impact of α-tocopherol (αT) and γ-tocopherol-rich tocopherols (γTmT) on gut inflammation, barrier integrity and microbiota in dextran sulfate sodium (DSS)-induced colitis in mice	438:619	The objective of this study is to examine the impact of α-tocopherol (αT) and γ-tocopherol-rich tocopherols (γTmT) on gut inflammation, barrier integrity and microbiota in dextran sulfate sodium (DSS)-induced colitis in mice.
33352218	6	39	theme	human	1102:1106	arg1	monolayer					1142:1150	human intestinal epithelial Caco-2 cell monolayer	1102:1150	human intestinal epithelial Caco-2 cell monolayer	1102:1150	Consistently, αT and γT mitigated TNF-α/IFN-γ-induced impairment of trans-epithelial electrical resistance in human intestinal epithelial Caco-2 cell monolayer.
33352218	11	40	theme	protective	1745:1754	arg1	effects					1756:1762	protective effects	1745:1762	protective effects of αT and γT on intestinal barrier function	1745:1806	These results provide evidence supporting protective effects of αT and γT on intestinal barrier function and that γTmT caused favorable changes of the gut microbiota in colitis-induced mice.
33352218	8	41	theme	Roseburia	1439:1447	arg1	depletion					1426:1434	DSS-caused depletion	1415:1434	DSS-caused depletion	1415:1434	In colitis-induced mice, γTmT but not αT separated gut microbial composition from controls, and attenuated DSS-caused depletion of Roseburia, which contains butyrate producing bacteria and is decreased in IBD patients.
33352218	3	42	theme	tocopherols	492:502	arg1	impact					442:447	the impact	438:447	the impact of α-tocopherol (αT) and γ-tocopherol-rich tocopherols (γTmT) on gut inflammation, barrier integrity and microbiota in dextran sulfate sodium (DSS)-induced colitis in mice	438:619	The objective of this study is to examine the impact of α-tocopherol (αT) and γ-tocopherol-rich tocopherols (γTmT) on gut inflammation, barrier integrity and microbiota in dextran sulfate sodium (DSS)-induced colitis in mice.
33352218	7	43	theme	fecal	1187:1191	arg1	DNA					1193:1195	fecal DNA	1187:1195	fecal DNA	1187:1195	Using 16S rRNA gene sequencing of fecal DNA, we observe that DSS reduced gut microbial evenness and separated microbial composition from healthy controls.
33352218	5	44	theme	gut	969:971	arg1	integrity					981:989	gut barrier integrity	969:989	gut barrier integrity	969:989	These vitamin E forms inhibited colitis-induced loss of the tight junction protein occludin, and attenuated colitis-caused elevation of LPS-binding protein in the plasma, a surrogate marker of intestinal barrier dysfunction, suggesting protection of gut barrier integrity.
33352218	6	45	theme	resistance	1088:1097	arg1	impairment					1046:1055	TNF-α/IFN-γ-induced impairment	1026:1055	TNF-α/IFN-γ-induced impairment of trans-epithelial electrical resistance in human intestinal epithelial Caco-2 cell monolayer	1026:1150	Consistently, αT and γT mitigated TNF-α/IFN-γ-induced impairment of trans-epithelial electrical resistance in human intestinal epithelial Caco-2 cell monolayer.
33352218	8	46	contain	contains	1456:1463	arg1	depletion					1426:1434	DSS-caused depletion	1415:1434	DSS-caused depletion	1415:1434	In colitis-induced mice, γTmT but not αT separated gut microbial composition from controls, and attenuated DSS-caused depletion of Roseburia, which contains butyrate producing bacteria and is decreased in IBD patients.
33352218	8	46	contain	contains	1456:1463	arg2	bacteria					1484:1491	butyrate producing bacteria	1465:1491	butyrate producing bacteria	1465:1491	In colitis-induced mice, γTmT but not αT separated gut microbial composition from controls, and attenuated DSS-caused depletion of Roseburia, which contains butyrate producing bacteria and is decreased in IBD patients.
33352218	0	47	from	microbiota	113:122	arg1	mice					127:130	mice	127:130	mice	127:130	Vitamin E alpha- and gamma-tocopherol mitigate colitis, protect intestinal barrier function and modulate the gut microbiota in mice.
33352218	1	48	theme	barrier	250:256	arg1	dysfunction					258:268	barrier dysfunction	250:268	barrier dysfunction	250:268	Inflammatory bowel diseases (IBDs) including colitis are intestinal disorders characterized by chronic inflammation, barrier dysfunction and dysbiosis.
33352218	6	49	theme	trans-epithelial	1060:1075	arg1	resistance					1088:1097	trans-epithelial electrical resistance	1060:1097	trans-epithelial electrical resistance	1060:1097	Consistently, αT and γT mitigated TNF-α/IFN-γ-induced impairment of trans-epithelial electrical resistance in human intestinal epithelial Caco-2 cell monolayer.
33352218	9	50	theme	correspondence	1537:1550	arg1	analysis					1552:1559	Canonical correspondence analysis	1527:1559	Canonical correspondence analysis	1527:1559	Canonical correspondence analysis also supports that γTmT favorably altered gut microbial community.
33352218	3	51	from	colitis	605:611	arg1	mice					616:619	mice	616:619	mice	616:619	The objective of this study is to examine the impact of α-tocopherol (αT) and γ-tocopherol-rich tocopherols (γTmT) on gut inflammation, barrier integrity and microbiota in dextran sulfate sodium (DSS)-induced colitis in mice.
33352218	6	52	from	impairment	1046:1055	arg1	monolayer					1142:1150	human intestinal epithelial Caco-2 cell monolayer	1102:1150	human intestinal epithelial Caco-2 cell monolayer	1102:1150	Consistently, αT and γT mitigated TNF-α/IFN-γ-induced impairment of trans-epithelial electrical resistance in human intestinal epithelial Caco-2 cell monolayer.
33352218	2	53	theme	Specific	285:292	arg1	forms					294:298	Specific forms	285:298	Specific forms of vitamin E	285:311	Specific forms of vitamin E have been shown to attenuate colitis, but the mechanisms are not fully understood.
33352218	3	54	theme	barrier	532:538	arg1	integrity					540:548	barrier integrity	532:548	barrier integrity	532:548	The objective of this study is to examine the impact of α-tocopherol (αT) and γ-tocopherol-rich tocopherols (γTmT) on gut inflammation, barrier integrity and microbiota in dextran sulfate sodium (DSS)-induced colitis in mice.
33352218	8	55	from	controls	1390:1397	arg1	composition					1373:1383	gut microbial composition	1359:1383	gut microbial composition from controls	1359:1397	In colitis-induced mice, γTmT but not αT separated gut microbial composition from controls, and attenuated DSS-caused depletion of Roseburia, which contains butyrate producing bacteria and is decreased in IBD patients.
33352218	11	56	theme	favorable	1829:1837	arg1	changes					1839:1845	favorable changes	1829:1845	favorable changes of the gut microbiota in colitis-induced mice	1829:1891	These results provide evidence supporting protective effects of αT and γT on intestinal barrier function and that γTmT caused favorable changes of the gut microbiota in colitis-induced mice.
33352218	2	57	theme	E	311:311	arg1	forms					294:298	Specific forms	285:298	Specific forms of vitamin E	285:311	Specific forms of vitamin E have been shown to attenuate colitis, but the mechanisms are not fully understood.
33352218	5	58	theme	tight	779:783	arg1	occludin					802:809	the tight junction protein occludin	775:809	the tight junction protein occludin	775:809	These vitamin E forms inhibited colitis-induced loss of the tight junction protein occludin, and attenuated colitis-caused elevation of LPS-binding protein in the plasma, a surrogate marker of intestinal barrier dysfunction, suggesting protection of gut barrier integrity.
33352218	5	59	theme	protein	794:800	arg1	occludin					802:809	the tight junction protein occludin	775:809	the tight junction protein occludin	775:809	These vitamin E forms inhibited colitis-induced loss of the tight junction protein occludin, and attenuated colitis-caused elevation of LPS-binding protein in the plasma, a surrogate marker of intestinal barrier dysfunction, suggesting protection of gut barrier integrity.
33352218	8	60	theme	butyrate	1465:1472	arg1	bacteria					1484:1491	butyrate producing bacteria	1465:1491	butyrate producing bacteria	1465:1491	In colitis-induced mice, γTmT but not αT separated gut microbial composition from controls, and attenuated DSS-caused depletion of Roseburia, which contains butyrate producing bacteria and is decreased in IBD patients.
33352218	11	61	theme	gut	1854:1856	arg1	microbiota					1858:1867	the gut microbiota	1850:1867	the gut microbiota	1850:1867	These results provide evidence supporting protective effects of αT and γT on intestinal barrier function and that γTmT caused favorable changes of the gut microbiota in colitis-induced mice.
33352218	3	62	from	inflammation	518:529	arg1	colitis					605:611	dextran sulfate sodium (DSS)-induced colitis	568:611	dextran sulfate sodium (DSS)-induced colitis in mice	568:619	The objective of this study is to examine the impact of α-tocopherol (αT) and γ-tocopherol-rich tocopherols (γTmT) on gut inflammation, barrier integrity and microbiota in dextran sulfate sodium (DSS)-induced colitis in mice.
33352218	6	63	theme	intestinal	1108:1117	arg1	monolayer					1142:1150	human intestinal epithelial Caco-2 cell monolayer	1102:1150	human intestinal epithelial Caco-2 cell monolayer	1102:1150	Consistently, αT and γT mitigated TNF-α/IFN-γ-induced impairment of trans-epithelial electrical resistance in human intestinal epithelial Caco-2 cell monolayer.
33352218	0	64	theme	intestinal	64:73	arg1	function					83:90	intestinal barrier function	64:90	intestinal barrier function	64:90	Vitamin E alpha- and gamma-tocopherol mitigate colitis, protect intestinal barrier function and modulate the gut microbiota in mice.
33352218	1	65	theme	Inflammatory	133:144	arg1	disorders					201:209	intestinal disorders	190:209	intestinal disorders characterized by chronic inflammation, barrier dysfunction and dysbiosis	190:282	Inflammatory bowel diseases (IBDs) including colitis are intestinal disorders characterized by chronic inflammation, barrier dysfunction and dysbiosis.
33352218	1	65	theme	Inflammatory	133:144	arg1	IBDs					162:165	IBDs	162:165	IBDs	162:165	Inflammatory bowel diseases (IBDs) including colitis are intestinal disorders characterized by chronic inflammation, barrier dysfunction and dysbiosis.
33352218	1	65	theme	Inflammatory	133:144	arg1	colitis					178:184	colitis	178:184	colitis	178:184	Inflammatory bowel diseases (IBDs) including colitis are intestinal disorders characterized by chronic inflammation, barrier dysfunction and dysbiosis.
33352218	1	65	theme	Inflammatory	133:144	arg1	diseases					152:159	Inflammatory bowel diseases	133:159	Inflammatory bowel diseases (IBDs) including colitis	133:184	Inflammatory bowel diseases (IBDs) including colitis are intestinal disorders characterized by chronic inflammation, barrier dysfunction and dysbiosis.
33352218	8	66	theme	colitis-induced	1311:1325	arg1	mice					1327:1330	colitis-induced mice	1311:1330	colitis-induced mice	1311:1330	In colitis-induced mice, γTmT but not αT separated gut microbial composition from controls, and attenuated DSS-caused depletion of Roseburia, which contains butyrate producing bacteria and is decreased in IBD patients.
33352218	5	67	theme	LPS-binding	855:865	arg1	marker					902:907	a surrogate marker	890:907	a surrogate marker of intestinal barrier dysfunction	890:941	These vitamin E forms inhibited colitis-induced loss of the tight junction protein occludin, and attenuated colitis-caused elevation of LPS-binding protein in the plasma, a surrogate marker of intestinal barrier dysfunction, suggesting protection of gut barrier integrity.
33352218	5	67	theme	LPS-binding	855:865	arg1	protein					867:873	LPS-binding protein	855:873	LPS-binding protein in the plasma	855:887	These vitamin E forms inhibited colitis-induced loss of the tight junction protein occludin, and attenuated colitis-caused elevation of LPS-binding protein in the plasma, a surrogate marker of intestinal barrier dysfunction, suggesting protection of gut barrier integrity.
33352218	5	68	from	protein	867:873	arg1	plasma					882:887	the plasma	878:887	the plasma	878:887	These vitamin E forms inhibited colitis-induced loss of the tight junction protein occludin, and attenuated colitis-caused elevation of LPS-binding protein in the plasma, a surrogate marker of intestinal barrier dysfunction, suggesting protection of gut barrier integrity.
33352218	5	69	theme	intestinal	912:921	arg1	dysfunction					931:941	intestinal barrier dysfunction	912:941	intestinal barrier dysfunction	912:941	These vitamin E forms inhibited colitis-induced loss of the tight junction protein occludin, and attenuated colitis-caused elevation of LPS-binding protein in the plasma, a surrogate marker of intestinal barrier dysfunction, suggesting protection of gut barrier integrity.
33352218	7	70	theme	microbial	1263:1271	arg1	composition					1273:1283	separated microbial composition	1253:1283	separated microbial composition	1253:1283	Using 16S rRNA gene sequencing of fecal DNA, we observe that DSS reduced gut microbial evenness and separated microbial composition from healthy controls.
33352218	0	71	theme	gut	109:111	arg1	microbiota					113:122	the gut microbiota	105:122	the gut microbiota in mice	105:130	Vitamin E alpha- and gamma-tocopherol mitigate colitis, protect intestinal barrier function and modulate the gut microbiota in mice.
33352218	1	72	theme	intestinal	190:199	arg1	diseases					152:159	Inflammatory bowel diseases	133:159	Inflammatory bowel diseases (IBDs) including colitis	133:184	Inflammatory bowel diseases (IBDs) including colitis are intestinal disorders characterized by chronic inflammation, barrier dysfunction and dysbiosis.
33352218	1	72	theme	intestinal	190:199	arg1	colitis					178:184	colitis	178:184	colitis	178:184	Inflammatory bowel diseases (IBDs) including colitis are intestinal disorders characterized by chronic inflammation, barrier dysfunction and dysbiosis.
33352218	1	72	theme	intestinal	190:199	arg1	disorders					201:209	intestinal disorders	190:209	intestinal disorders characterized by chronic inflammation, barrier dysfunction and dysbiosis	190:282	Inflammatory bowel diseases (IBDs) including colitis are intestinal disorders characterized by chronic inflammation, barrier dysfunction and dysbiosis.
33352218	5	73	theme	dysfunction	931:941	arg1	marker					902:907	a surrogate marker	890:907	a surrogate marker of intestinal barrier dysfunction	890:941	These vitamin E forms inhibited colitis-induced loss of the tight junction protein occludin, and attenuated colitis-caused elevation of LPS-binding protein in the plasma, a surrogate marker of intestinal barrier dysfunction, suggesting protection of gut barrier integrity.
33352218	5	73	theme	dysfunction	931:941	arg1	protein					867:873	LPS-binding protein	855:873	LPS-binding protein in the plasma	855:887	These vitamin E forms inhibited colitis-induced loss of the tight junction protein occludin, and attenuated colitis-caused elevation of LPS-binding protein in the plasma, a surrogate marker of intestinal barrier dysfunction, suggesting protection of gut barrier integrity.
33352218	11	74	theme	barrier	1791:1797	arg1	function					1799:1806	intestinal barrier function	1780:1806	intestinal barrier function	1780:1806	These results provide evidence supporting protective effects of αT and γT on intestinal barrier function and that γTmT caused favorable changes of the gut microbiota in colitis-induced mice.
33352218	10	75	theme	gut	1670:1672	arg1	microbes					1674:1681	gut microbes	1670:1681	gut microbes	1670:1681	In contrast, neither αT nor γTmT affected gut microbes in healthy animals.
33352218	5	76	theme	colitis-induced	751:765	arg1	loss					767:770	colitis-induced loss	751:770	colitis-induced loss of the tight junction protein occludin	751:809	These vitamin E forms inhibited colitis-induced loss of the tight junction protein occludin, and attenuated colitis-caused elevation of LPS-binding protein in the plasma, a surrogate marker of intestinal barrier dysfunction, suggesting protection of gut barrier integrity.
33352218	6	77	theme	TNF-α/IFN-γ-induced	1026:1044	arg1	impairment					1046:1055	TNF-α/IFN-γ-induced impairment	1026:1055	TNF-α/IFN-γ-induced impairment of trans-epithelial electrical resistance in human intestinal epithelial Caco-2 cell monolayer	1026:1150	Consistently, αT and γT mitigated TNF-α/IFN-γ-induced impairment of trans-epithelial electrical resistance in human intestinal epithelial Caco-2 cell monolayer.
33352218	8	78	theme	gut	1359:1361	arg1	composition					1373:1383	gut microbial composition	1359:1383	gut microbial composition from controls	1359:1397	In colitis-induced mice, γTmT but not αT separated gut microbial composition from controls, and attenuated DSS-caused depletion of Roseburia, which contains butyrate producing bacteria and is decreased in IBD patients.
33352218	3	79	theme	study	418:422	arg1	objective					400:408	The objective	396:408	The objective of this study	396:422	The objective of this study is to examine the impact of α-tocopherol (αT) and γ-tocopherol-rich tocopherols (γTmT) on gut inflammation, barrier integrity and microbiota in dextran sulfate sodium (DSS)-induced colitis in mice.
33352218	7	80	theme	rRNA	1163:1166	arg1	sequencing					1173:1182	16S rRNA gene sequencing	1159:1182	16S rRNA gene sequencing of fecal DNA	1159:1195	Using 16S rRNA gene sequencing of fecal DNA, we observe that DSS reduced gut microbial evenness and separated microbial composition from healthy controls.
33352218	9	81	theme	gut	1603:1605	arg1	community					1617:1625	gut microbial community	1603:1625	gut microbial community	1603:1625	Canonical correspondence analysis also supports that γTmT favorably altered gut microbial community.
33352218	5	82	theme	vitamin	725:731	arg1	forms					735:739	These vitamin E forms	719:739	These vitamin E forms	719:739	These vitamin E forms inhibited colitis-induced loss of the tight junction protein occludin, and attenuated colitis-caused elevation of LPS-binding protein in the plasma, a surrogate marker of intestinal barrier dysfunction, suggesting protection of gut barrier integrity.
33352218	5	83	theme	barrier	973:979	arg1	integrity					981:989	gut barrier integrity	969:989	gut barrier integrity	969:989	These vitamin E forms inhibited colitis-induced loss of the tight junction protein occludin, and attenuated colitis-caused elevation of LPS-binding protein in the plasma, a surrogate marker of intestinal barrier dysfunction, suggesting protection of gut barrier integrity.
33352218	7	84	theme	healthy	1290:1296	arg1	controls					1298:1305	healthy controls	1290:1305	healthy controls	1290:1305	Using 16S rRNA gene sequencing of fecal DNA, we observe that DSS reduced gut microbial evenness and separated microbial composition from healthy controls.
33352218	7	85	from	controls	1298:1305	arg1	evenness					1240:1247	gut microbial evenness	1226:1247	gut microbial evenness	1226:1247	Using 16S rRNA gene sequencing of fecal DNA, we observe that DSS reduced gut microbial evenness and separated microbial composition from healthy controls.
33352218	7	85	from	controls	1298:1305	arg1	composition					1273:1283	separated microbial composition	1253:1283	separated microbial composition	1253:1283	Using 16S rRNA gene sequencing of fecal DNA, we observe that DSS reduced gut microbial evenness and separated microbial composition from healthy controls.
33352218	6	86	theme	Caco-2 cell	1130:1140	arg1	monolayer					1142:1150	human intestinal epithelial Caco-2 cell monolayer	1102:1150	human intestinal epithelial Caco-2 cell monolayer	1102:1150	Consistently, αT and γT mitigated TNF-α/IFN-γ-induced impairment of trans-epithelial electrical resistance in human intestinal epithelial Caco-2 cell monolayer.
33352218	1	87	theme	bowel	146:150	arg1	disorders					201:209	intestinal disorders	190:209	intestinal disorders characterized by chronic inflammation, barrier dysfunction and dysbiosis	190:282	Inflammatory bowel diseases (IBDs) including colitis are intestinal disorders characterized by chronic inflammation, barrier dysfunction and dysbiosis.
33352218	1	87	theme	bowel	146:150	arg1	IBDs					162:165	IBDs	162:165	IBDs	162:165	Inflammatory bowel diseases (IBDs) including colitis are intestinal disorders characterized by chronic inflammation, barrier dysfunction and dysbiosis.
33352218	1	87	theme	bowel	146:150	arg1	colitis					178:184	colitis	178:184	colitis	178:184	Inflammatory bowel diseases (IBDs) including colitis are intestinal disorders characterized by chronic inflammation, barrier dysfunction and dysbiosis.
33352218	1	87	theme	bowel	146:150	arg1	diseases					152:159	Inflammatory bowel diseases	133:159	Inflammatory bowel diseases (IBDs) including colitis	133:184	Inflammatory bowel diseases (IBDs) including colitis are intestinal disorders characterized by chronic inflammation, barrier dysfunction and dysbiosis.
33352218	3	88	from	impact	442:447	arg1	microbiota					554:563	microbiota	554:563	microbiota	554:563	The objective of this study is to examine the impact of α-tocopherol (αT) and γ-tocopherol-rich tocopherols (γTmT) on gut inflammation, barrier integrity and microbiota in dextran sulfate sodium (DSS)-induced colitis in mice.
33352218	3	88	from	impact	442:447	arg1	inflammation					518:529	gut inflammation	514:529	gut inflammation	514:529	The objective of this study is to examine the impact of α-tocopherol (αT) and γ-tocopherol-rich tocopherols (γTmT) on gut inflammation, barrier integrity and microbiota in dextran sulfate sodium (DSS)-induced colitis in mice.
33352218	3	88	from	impact	442:447	arg1	integrity					540:548	barrier integrity	532:548	barrier integrity	532:548	The objective of this study is to examine the impact of α-tocopherol (αT) and γ-tocopherol-rich tocopherols (γTmT) on gut inflammation, barrier integrity and microbiota in dextran sulfate sodium (DSS)-induced colitis in mice.
33352218	7	89	theme	separated	1253:1261	arg1	composition					1273:1283	separated microbial composition	1253:1283	separated microbial composition	1253:1283	Using 16S rRNA gene sequencing of fecal DNA, we observe that DSS reduced gut microbial evenness and separated microbial composition from healthy controls.
33352218	8	90	theme	DSS-caused	1415:1424	arg1	depletion					1426:1434	DSS-caused depletion	1415:1434	DSS-caused depletion	1415:1434	In colitis-induced mice, γTmT but not αT separated gut microbial composition from controls, and attenuated DSS-caused depletion of Roseburia, which contains butyrate producing bacteria and is decreased in IBD patients.
33352218	1	91	theme	chronic	228:234	arg1	inflammation					236:247	chronic inflammation	228:247	chronic inflammation	228:247	Inflammatory bowel diseases (IBDs) including colitis are intestinal disorders characterized by chronic inflammation, barrier dysfunction and dysbiosis.
33352218	0	92	theme	E	8:8	arg1	alpha-					10:15	Vitamin E alpha-	0:15	Vitamin E alpha-	0:15	Vitamin E alpha- and gamma-tocopherol mitigate colitis, protect intestinal barrier function and modulate the gut microbiota in mice.
33352218	7	93	theme	gut	1226:1228	arg1	evenness					1240:1247	gut microbial evenness	1226:1247	gut microbial evenness	1226:1247	Using 16S rRNA gene sequencing of fecal DNA, we observe that DSS reduced gut microbial evenness and separated microbial composition from healthy controls.
34105571	0	0	theme	gut	87:89	arg1	microbiota					91:100	gut microbiota	87:100	gut microbiota	87:100	A polysaccharide from natural Cordyceps sinensis regulates the intestinal immunity and gut microbiota in mice with cyclophosphamide-induced intestinal injury.
34105571	4	1	theme	cells	643:647	arg1	number					626:631	the number	622:631	the number of CD4+ T cells	622:647	NCSP was found to increase the number of CD4+ T cells, stimulate the secretion of interleukins (IL)-17 and IL-21, and the expression of transcription factor (retinoic acid-related orphan receptor (ROR)-γt).
34105571	6	2	theme	mRNA	971:974	arg1	expression					976:985	the mRNA expression	967:985	the mRNA expression of toll	967:993	Moreover, NCSP upregulated the mRNA expression of toll like receptors (TLR-2, -6 and -9), while it downregulated the TLR-4 expression.
34105571	1	3	theme	Cordyceps	181:189	arg1	NCSP					201:204	NCSP	201:204	NCSP	201:204	A polysaccharide from Cordyceps sinensis (NCSP) was reported to attenuate intestinal injury and regulate the balance of T helper (Th)1/Th2 cells in immunosuppressed mice.
34105571	1	3	theme	Cordyceps	181:189	arg1	sinensis					191:198	Cordyceps sinensis	181:198	Cordyceps sinensis (NCSP)	181:205	A polysaccharide from Cordyceps sinensis (NCSP) was reported to attenuate intestinal injury and regulate the balance of T helper (Th)1/Th2 cells in immunosuppressed mice.
34105571	8	4	theme	intestinal	1223:1232	arg1	immunity					1234:1241	intestinal immunity	1223:1241	intestinal immunity	1223:1241	These findings indicated that NCSP may enhance intestinal immunity and have the potential to become a prebiotic to regulate intestinal microbiota.
34105571	5	5	theme	transcription	856:868	arg1	p-3					896:898	forkhead box (Fox)p-3	878:898	forkhead box (Fox)p-3	878:898	The levels of transforming growth factor (TGF)-β3 and transcription factor (forkhead box (Fox)p-3) were increased in NCSP-treated groups.
34105571	5	5	theme	transcription	856:868	arg1	factor					870:875	transcription factor	856:875	transcription factor (forkhead box (Fox)p-3)	856:899	The levels of transforming growth factor (TGF)-β3 and transcription factor (forkhead box (Fox)p-3) were increased in NCSP-treated groups.
34105571	1	6	theme	T	279:279	arg1	Th					289:290	Th	289:290	Th	289:290	A polysaccharide from Cordyceps sinensis (NCSP) was reported to attenuate intestinal injury and regulate the balance of T helper (Th)1/Th2 cells in immunosuppressed mice.
34105571	1	6	theme	T	279:279	arg1	helper					281:286	T helper	279:286	T helper (Th)1/Th2 cells	279:302	A polysaccharide from Cordyceps sinensis (NCSP) was reported to attenuate intestinal injury and regulate the balance of T helper (Th)1/Th2 cells in immunosuppressed mice.
34105571	4	7	theme	T	641:641	arg1	cells					643:647	CD4+ T cells	636:647	CD4+ T cells	636:647	NCSP was found to increase the number of CD4+ T cells, stimulate the secretion of interleukins (IL)-17 and IL-21, and the expression of transcription factor (retinoic acid-related orphan receptor (ROR)-γt).
34105571	4	8	theme	acid-related	762:773	arg1	ROR					792:794	ROR	792:794	ROR	792:794	NCSP was found to increase the number of CD4+ T cells, stimulate the secretion of interleukins (IL)-17 and IL-21, and the expression of transcription factor (retinoic acid-related orphan receptor (ROR)-γt).
34105571	4	8	theme	acid-related	762:773	arg1	receptor					782:789	retinoic acid-related orphan receptor	753:789	retinoic acid-related orphan receptor (ROR)-γt	753:798	NCSP was found to increase the number of CD4+ T cells, stimulate the secretion of interleukins (IL)-17 and IL-21, and the expression of transcription factor (retinoic acid-related orphan receptor (ROR)-γt).
34105571	5	9	theme	factor	870:875	arg1	levels					806:811	The levels	802:811	The levels of transforming growth factor (TGF)-β3 and transcription factor (forkhead box (Fox)p-3)	802:899	The levels of transforming growth factor (TGF)-β3 and transcription factor (forkhead box (Fox)p-3) were increased in NCSP-treated groups.
34105571	0	10	from	sinensis	40:47	arg1	polysaccharide					2:15	A polysaccharide	0:15	A polysaccharide from natural Cordyceps sinensis	0:47	A polysaccharide from natural Cordyceps sinensis regulates the intestinal immunity and gut microbiota in mice with cyclophosphamide-induced intestinal injury.
34105571	4	11	theme	retinoic	753:760	arg1	ROR					792:794	ROR	792:794	ROR	792:794	NCSP was found to increase the number of CD4+ T cells, stimulate the secretion of interleukins (IL)-17 and IL-21, and the expression of transcription factor (retinoic acid-related orphan receptor (ROR)-γt).
34105571	4	11	theme	retinoic	753:760	arg1	receptor					782:789	retinoic acid-related orphan receptor	753:789	retinoic acid-related orphan receptor (ROR)-γt	753:798	NCSP was found to increase the number of CD4+ T cells, stimulate the secretion of interleukins (IL)-17 and IL-21, and the expression of transcription factor (retinoic acid-related orphan receptor (ROR)-γt).
34105571	2	12	theme	T	381:381	arg1	cells					390:394	regulatory T (Treg) cells	370:394	regulatory T (Treg) cells	370:394	However, whether it influences Th17 and regulatory T (Treg) cells as well as gut ecology remains unknown.
34105571	4	13	theme	receptor	782:789	arg1	factor					745:750	transcription factor	731:750	transcription factor (retinoic acid-related orphan receptor (ROR)-γt)	731:799	NCSP was found to increase the number of CD4+ T cells, stimulate the secretion of interleukins (IL)-17 and IL-21, and the expression of transcription factor (retinoic acid-related orphan receptor (ROR)-γt).
34105571	4	13	theme	receptor	782:789	arg1	-γt					796:798	retinoic acid-related orphan receptor (ROR)-γt	753:798	retinoic acid-related orphan receptor (ROR)-γt	753:798	NCSP was found to increase the number of CD4+ T cells, stimulate the secretion of interleukins (IL)-17 and IL-21, and the expression of transcription factor (retinoic acid-related orphan receptor (ROR)-γt).
34105571	0	14	with	mice	105:108	arg1	injury					151:156	cyclophosphamide-induced intestinal injury	115:156	cyclophosphamide-induced intestinal injury	115:156	A polysaccharide from natural Cordyceps sinensis regulates the intestinal immunity and gut microbiota in mice with cyclophosphamide-induced intestinal injury.
34105571	4	15	theme	interleukins	677:688	arg1	expression					717:726	the expression	713:726	the expression of transcription factor (retinoic acid-related orphan receptor (ROR)-γt)	713:799	NCSP was found to increase the number of CD4+ T cells, stimulate the secretion of interleukins (IL)-17 and IL-21, and the expression of transcription factor (retinoic acid-related orphan receptor (ROR)-γt).
34105571	4	15	theme	interleukins	677:688	arg1	secretion					664:672	the secretion	660:672	the secretion of interleukins (IL)-17 and IL-21	660:706	NCSP was found to increase the number of CD4+ T cells, stimulate the secretion of interleukins (IL)-17 and IL-21, and the expression of transcription factor (retinoic acid-related orphan receptor (ROR)-γt).
34105571	7	16	theme	intestinal	1107:1116	arg1	composition					1129:1139	the intestinal microbiota composition	1103:1139	the intestinal microbiota composition	1103:1139	In addition, NCSP modulated the intestinal microbiota composition and increased the levels of SCFAs.
34105571	2	17	theme	regulatory	370:379	arg1	cells					390:394	regulatory T (Treg) cells	370:394	regulatory T (Treg) cells	370:394	However, whether it influences Th17 and regulatory T (Treg) cells as well as gut ecology remains unknown.
34105571	2	18	theme	gut	407:409	arg1	ecology					411:417	gut ecology	407:417	Th17 and regulatory T (Treg) cells as well as gut ecology	361:417	However, whether it influences Th17 and regulatory T (Treg) cells as well as gut ecology remains unknown.
34105571	1	19	theme	helper	281:286	arg1	cells					298:302	T helper (Th)1/Th2 cells	279:302	T helper (Th)1/Th2 cells	279:302	A polysaccharide from Cordyceps sinensis (NCSP) was reported to attenuate intestinal injury and regulate the balance of T helper (Th)1/Th2 cells in immunosuppressed mice.
34105571	4	20	theme	orphan	775:780	arg1	ROR					792:794	ROR	792:794	ROR	792:794	NCSP was found to increase the number of CD4+ T cells, stimulate the secretion of interleukins (IL)-17 and IL-21, and the expression of transcription factor (retinoic acid-related orphan receptor (ROR)-γt).
34105571	4	20	theme	orphan	775:780	arg1	receptor					782:789	retinoic acid-related orphan receptor	753:789	retinoic acid-related orphan receptor (ROR)-γt	753:798	NCSP was found to increase the number of CD4+ T cells, stimulate the secretion of interleukins (IL)-17 and IL-21, and the expression of transcription factor (retinoic acid-related orphan receptor (ROR)-γt).
34105571	7	21	theme	SCFAs	1169:1173	arg1	levels					1159:1164	the levels	1155:1164	the levels of SCFAs	1155:1173	In addition, NCSP modulated the intestinal microbiota composition and increased the levels of SCFAs.
34105571	0	22	theme	natural	22:28	arg1	sinensis					40:47	natural Cordyceps sinensis	22:47	natural Cordyceps sinensis	22:47	A polysaccharide from natural Cordyceps sinensis regulates the intestinal immunity and gut microbiota in mice with cyclophosphamide-induced intestinal injury.
34105571	6	23	dep	receptors	1000:1008	arg1	-9					1025:1026	-9	1025:1026	-9	1025:1026	Moreover, NCSP upregulated the mRNA expression of toll like receptors (TLR-2, -6 and -9), while it downregulated the TLR-4 expression.
34105571	6	23	dep	receptors	1000:1008	arg1	-6					1018:1019	-6	1018:1019	-6	1018:1019	Moreover, NCSP upregulated the mRNA expression of toll like receptors (TLR-2, -6 and -9), while it downregulated the TLR-4 expression.
34105571	6	23	dep	receptors	1000:1008	arg1	TLR-2					1011:1015	TLR-2	1011:1015	TLR-2	1011:1015	Moreover, NCSP upregulated the mRNA expression of toll like receptors (TLR-2, -6 and -9), while it downregulated the TLR-4 expression.
34105571	0	24	theme	intestinal	140:149	arg1	injury					151:156	cyclophosphamide-induced intestinal injury	115:156	cyclophosphamide-induced intestinal injury	115:156	A polysaccharide from natural Cordyceps sinensis regulates the intestinal immunity and gut microbiota in mice with cyclophosphamide-induced intestinal injury.
34105571	5	25	theme	factor	836:841	arg1	levels					806:811	The levels	802:811	The levels of transforming growth factor (TGF)-β3 and transcription factor (forkhead box (Fox)p-3)	802:899	The levels of transforming growth factor (TGF)-β3 and transcription factor (forkhead box (Fox)p-3) were increased in NCSP-treated groups.
34105571	0	26	dep	immunity	74:81	arg1	the					59:61	the	59:61	the	59:61	A polysaccharide from natural Cordyceps sinensis regulates the intestinal immunity and gut microbiota in mice with cyclophosphamide-induced intestinal injury.
34105571	0	27	theme	cyclophosphamide-induced	115:138	arg1	injury					151:156	cyclophosphamide-induced intestinal injury	115:156	cyclophosphamide-induced intestinal injury	115:156	A polysaccharide from natural Cordyceps sinensis regulates the intestinal immunity and gut microbiota in mice with cyclophosphamide-induced intestinal injury.
34105571	3	28	theme	intraperitoneal	516:530	arg1	injection					532:540	intraperitoneal injection	516:540	intraperitoneal injection of cyclophosphamide (Cy)	516:565	In the present study, the intestinal injury mouse model was also established by intraperitoneal injection of cyclophosphamide (Cy) for three consecutive days.
34105571	4	29	theme	CD4+	636:639	arg1	cells					643:647	CD4+ T cells	636:647	CD4+ T cells	636:647	NCSP was found to increase the number of CD4+ T cells, stimulate the secretion of interleukins (IL)-17 and IL-21, and the expression of transcription factor (retinoic acid-related orphan receptor (ROR)-γt).
34105571	4	30	theme	transcription	731:743	arg1	factor					745:750	transcription factor	731:750	transcription factor (retinoic acid-related orphan receptor (ROR)-γt)	731:799	NCSP was found to increase the number of CD4+ T cells, stimulate the secretion of interleukins (IL)-17 and IL-21, and the expression of transcription factor (retinoic acid-related orphan receptor (ROR)-γt).
34105571	4	30	theme	transcription	731:743	arg1	-γt					796:798	retinoic acid-related orphan receptor (ROR)-γt	753:798	retinoic acid-related orphan receptor (ROR)-γt	753:798	NCSP was found to increase the number of CD4+ T cells, stimulate the secretion of interleukins (IL)-17 and IL-21, and the expression of transcription factor (retinoic acid-related orphan receptor (ROR)-γt).
34105571	0	31	theme	Cordyceps	30:38	arg1	sinensis					40:47	natural Cordyceps sinensis	22:47	natural Cordyceps sinensis	22:47	A polysaccharide from natural Cordyceps sinensis regulates the intestinal immunity and gut microbiota in mice with cyclophosphamide-induced intestinal injury.
34105571	1	32	theme	1/Th2	292:296	arg1	cells					298:302	T helper (Th)1/Th2 cells	279:302	T helper (Th)1/Th2 cells	279:302	A polysaccharide from Cordyceps sinensis (NCSP) was reported to attenuate intestinal injury and regulate the balance of T helper (Th)1/Th2 cells in immunosuppressed mice.
34105571	1	33	from	balance	268:274	arg1	mice					324:327	immunosuppressed mice	307:327	immunosuppressed mice	307:327	A polysaccharide from Cordyceps sinensis (NCSP) was reported to attenuate intestinal injury and regulate the balance of T helper (Th)1/Th2 cells in immunosuppressed mice.
34105571	0	34	from	immunity	74:81	arg1	mice					105:108	mice	105:108	mice with cyclophosphamide-induced intestinal injury	105:156	A polysaccharide from natural Cordyceps sinensis regulates the intestinal immunity and gut microbiota in mice with cyclophosphamide-induced intestinal injury.
34105571	2	35	theme	Treg	384:387	arg1	cells					390:394	regulatory T (Treg) cells	370:394	regulatory T (Treg) cells	370:394	However, whether it influences Th17 and regulatory T (Treg) cells as well as gut ecology remains unknown.
34105571	1	36	theme	cells	298:302	arg1	balance					268:274	the balance	264:274	the balance of T helper (Th)1/Th2 cells in immunosuppressed mice	264:327	A polysaccharide from Cordyceps sinensis (NCSP) was reported to attenuate intestinal injury and regulate the balance of T helper (Th)1/Th2 cells in immunosuppressed mice.
34105571	8	37	theme	intestinal	1300:1309	arg1	microbiota					1311:1320	intestinal microbiota	1300:1320	intestinal microbiota	1300:1320	These findings indicated that NCSP may enhance intestinal immunity and have the potential to become a prebiotic to regulate intestinal microbiota.
34105571	3	38	theme	cyclophosphamide	545:560	arg1	injection					532:540	intraperitoneal injection	516:540	intraperitoneal injection of cyclophosphamide (Cy)	516:565	In the present study, the intestinal injury mouse model was also established by intraperitoneal injection of cyclophosphamide (Cy) for three consecutive days.
34105571	6	39	theme	toll	990:993	arg1	expression					976:985	the mRNA expression	967:985	the mRNA expression of toll	967:993	Moreover, NCSP upregulated the mRNA expression of toll like receptors (TLR-2, -6 and -9), while it downregulated the TLR-4 expression.
34105571	7	40	theme	microbiota	1118:1127	arg1	composition					1129:1139	the intestinal microbiota composition	1103:1139	the intestinal microbiota composition	1103:1139	In addition, NCSP modulated the intestinal microbiota composition and increased the levels of SCFAs.
34105571	8	41	contain	have	1247:1250	arg1	NCSP					1206:1209	NCSP	1206:1209	NCSP	1206:1209	These findings indicated that NCSP may enhance intestinal immunity and have the potential to become a prebiotic to regulate intestinal microbiota.
34105571	8	41	contain	have	1247:1250	arg2	potential					1256:1264	the potential to become a prebiotic to regulate intestinal microbiota	1252:1320	the potential to become a prebiotic to regulate intestinal microbiota	1252:1320	These findings indicated that NCSP may enhance intestinal immunity and have the potential to become a prebiotic to regulate intestinal microbiota.
34105571	4	42	theme	factor	745:750	arg1	expression					717:726	the expression	713:726	the expression of transcription factor (retinoic acid-related orphan receptor (ROR)-γt)	713:799	NCSP was found to increase the number of CD4+ T cells, stimulate the secretion of interleukins (IL)-17 and IL-21, and the expression of transcription factor (retinoic acid-related orphan receptor (ROR)-γt).
34105571	4	42	theme	factor	745:750	arg1	secretion					664:672	the secretion	660:672	the secretion of interleukins (IL)-17 and IL-21	660:706	NCSP was found to increase the number of CD4+ T cells, stimulate the secretion of interleukins (IL)-17 and IL-21, and the expression of transcription factor (retinoic acid-related orphan receptor (ROR)-γt).
34105571	3	43	theme	intestinal	462:471	arg1	model					486:490	the intestinal injury mouse model	458:490	the intestinal injury mouse model	458:490	In the present study, the intestinal injury mouse model was also established by intraperitoneal injection of cyclophosphamide (Cy) for three consecutive days.
34105571	5	44	theme	transforming	816:827	arg1	TGF					844:846	TGF	844:846	TGF	844:846	The levels of transforming growth factor (TGF)-β3 and transcription factor (forkhead box (Fox)p-3) were increased in NCSP-treated groups.
34105571	5	44	theme	transforming	816:827	arg1	factor					836:841	transforming growth factor (TGF)-β3	816:850	transforming growth factor (TGF)-β3	816:850	The levels of transforming growth factor (TGF)-β3 and transcription factor (forkhead box (Fox)p-3) were increased in NCSP-treated groups.
34105571	6	45	theme	TLR-4	1057:1061	arg1	expression					1063:1072	the TLR-4 expression	1053:1072	the TLR-4 expression	1053:1072	Moreover, NCSP upregulated the mRNA expression of toll like receptors (TLR-2, -6 and -9), while it downregulated the TLR-4 expression.
34105571	1	46	theme	immunosuppressed	307:322	arg1	mice					324:327	immunosuppressed mice	307:327	immunosuppressed mice	307:327	A polysaccharide from Cordyceps sinensis (NCSP) was reported to attenuate intestinal injury and regulate the balance of T helper (Th)1/Th2 cells in immunosuppressed mice.
34105571	5	47	theme	forkhead	878:885	arg1	p-3					896:898	forkhead box (Fox)p-3	878:898	forkhead box (Fox)p-3	878:898	The levels of transforming growth factor (TGF)-β3 and transcription factor (forkhead box (Fox)p-3) were increased in NCSP-treated groups.
34105571	5	47	theme	forkhead	878:885	arg1	factor					870:875	transcription factor	856:875	transcription factor (forkhead box (Fox)p-3)	856:899	The levels of transforming growth factor (TGF)-β3 and transcription factor (forkhead box (Fox)p-3) were increased in NCSP-treated groups.
34105571	3	48	theme	consecutive	577:587	arg1	days					589:592	three consecutive days	571:592	three consecutive days	571:592	In the present study, the intestinal injury mouse model was also established by intraperitoneal injection of cyclophosphamide (Cy) for three consecutive days.
34105571	4	49	dep	interleukins	677:688	arg1	-17					694:696	-17	694:696	-17	694:696	NCSP was found to increase the number of CD4+ T cells, stimulate the secretion of interleukins (IL)-17 and IL-21, and the expression of transcription factor (retinoic acid-related orphan receptor (ROR)-γt).
34105571	4	49	dep	interleukins	677:688	arg1	IL-21					702:706	IL-21	702:706	IL-21	702:706	NCSP was found to increase the number of CD4+ T cells, stimulate the secretion of interleukins (IL)-17 and IL-21, and the expression of transcription factor (retinoic acid-related orphan receptor (ROR)-γt).
34105571	1	50	theme	intestinal	233:242	arg1	injury					244:249	intestinal injury	233:249	intestinal injury	233:249	A polysaccharide from Cordyceps sinensis (NCSP) was reported to attenuate intestinal injury and regulate the balance of T helper (Th)1/Th2 cells in immunosuppressed mice.
34105571	5	51	theme	NCSP-treated	919:930	arg1	groups					932:937	NCSP-treated groups	919:937	NCSP-treated groups	919:937	The levels of transforming growth factor (TGF)-β3 and transcription factor (forkhead box (Fox)p-3) were increased in NCSP-treated groups.
34105571	5	52	theme	box	887:889	arg1	p-3					896:898	forkhead box (Fox)p-3	878:898	forkhead box (Fox)p-3	878:898	The levels of transforming growth factor (TGF)-β3 and transcription factor (forkhead box (Fox)p-3) were increased in NCSP-treated groups.
34105571	5	52	theme	box	887:889	arg1	factor					870:875	transcription factor	856:875	transcription factor (forkhead box (Fox)p-3)	856:899	The levels of transforming growth factor (TGF)-β3 and transcription factor (forkhead box (Fox)p-3) were increased in NCSP-treated groups.
34105571	3	53	theme	present	443:449	arg1	study					451:455	the present study	439:455	the present study	439:455	In the present study, the intestinal injury mouse model was also established by intraperitoneal injection of cyclophosphamide (Cy) for three consecutive days.
34105571	5	54	theme	growth	829:834	arg1	TGF					844:846	TGF	844:846	TGF	844:846	The levels of transforming growth factor (TGF)-β3 and transcription factor (forkhead box (Fox)p-3) were increased in NCSP-treated groups.
34105571	5	54	theme	growth	829:834	arg1	factor					836:841	transforming growth factor (TGF)-β3	816:850	transforming growth factor (TGF)-β3	816:850	The levels of transforming growth factor (TGF)-β3 and transcription factor (forkhead box (Fox)p-3) were increased in NCSP-treated groups.
34105571	0	55	from	microbiota	91:100	arg1	mice					105:108	mice	105:108	mice with cyclophosphamide-induced intestinal injury	105:156	A polysaccharide from natural Cordyceps sinensis regulates the intestinal immunity and gut microbiota in mice with cyclophosphamide-induced intestinal injury.
34105571	4	56	dep	increase	613:620	arg1	stimulate					650:658	stimulate	650:658	stimulate the secretion of interleukins (IL)-17 and IL-21, and the expression of transcription factor (retinoic acid-related orphan receptor (ROR)-γt)	650:799	NCSP was found to increase the number of CD4+ T cells, stimulate the secretion of interleukins (IL)-17 and IL-21, and the expression of transcription factor (retinoic acid-related orphan receptor (ROR)-γt).
34105571	3	57	theme	injury	473:478	arg1	model					486:490	the intestinal injury mouse model	458:490	the intestinal injury mouse model	458:490	In the present study, the intestinal injury mouse model was also established by intraperitoneal injection of cyclophosphamide (Cy) for three consecutive days.
34105571	0	58	theme	intestinal	63:72	arg1	immunity					74:81	intestinal immunity	63:81	intestinal immunity	63:81	A polysaccharide from natural Cordyceps sinensis regulates the intestinal immunity and gut microbiota in mice with cyclophosphamide-induced intestinal injury.
34105571	5	59	theme	Fox	892:894	arg1	p-3					896:898	forkhead box (Fox)p-3	878:898	forkhead box (Fox)p-3	878:898	The levels of transforming growth factor (TGF)-β3 and transcription factor (forkhead box (Fox)p-3) were increased in NCSP-treated groups.
34105571	5	59	theme	Fox	892:894	arg1	factor					870:875	transcription factor	856:875	transcription factor (forkhead box (Fox)p-3)	856:899	The levels of transforming growth factor (TGF)-β3 and transcription factor (forkhead box (Fox)p-3) were increased in NCSP-treated groups.
34105571	1	60	from	sinensis	191:198	arg1	polysaccharide					161:174	A polysaccharide	159:174	A polysaccharide from Cordyceps sinensis (NCSP)	159:205	A polysaccharide from Cordyceps sinensis (NCSP) was reported to attenuate intestinal injury and regulate the balance of T helper (Th)1/Th2 cells in immunosuppressed mice.
34105571	3	61	theme	mouse	480:484	arg1	model					486:490	the intestinal injury mouse model	458:490	the intestinal injury mouse model	458:490	In the present study, the intestinal injury mouse model was also established by intraperitoneal injection of cyclophosphamide (Cy) for three consecutive days.
33142500	7	0	theme	ATP	1249:1251	arg1	citrate-lyase					1253:1265	ATP citrate-lyase	1249:1265	ATP citrate-lyase	1249:1265	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	10	1	theme	acid	2017:2020	arg1	synthesis					2022:2030	liver fatty acid synthesis	2005:2030	liver fatty acid synthesis	2005:2030	Overall, broilers receiving diets containing a higher concentration of RS harbor less Firmicutes, which decreased liver fatty acid synthesis and suppress abdominal fat deposition of birds during the starter phase.
33142500	2	2	theme	dietary	372:378	arg1	supplementation					451:465	1 corn-soybean-based diet supplementation	425:465	1 corn-soybean-based diet supplementation with 20% corn starch (CS)	425:491	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	2	2	theme	dietary	372:378	arg1	treatments					380:389	5 dietary treatments	370:389	5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively)	370:637	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	2	2	theme	dietary	372:378	arg1	diet					419:422	1 normal corn-soybean (NC) diet	392:422	1 normal corn-soybean (NC) diet	392:422	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	7	3	from	synthase	1279:1286	arg1	liver					1319:1323	liver	1319:1323	liver	1319:1323	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	7	3	from	synthase	1279:1286	arg1	tissue					1388:1393	abdominal adipose tissue	1370:1393	abdominal adipose tissue (P < 0.05)	1370:1404	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	7	3	from	synthase	1279:1286	arg1	P < 0.05					1396:1403	P < 0.05	1396:1403	P < 0.05	1396:1403	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	7	4	theme	gamma	1242:1246	arg1	expressions					1184:1194	the relative mRNA expressions	1166:1194	the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05)	1166:1404	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	2	5	theme	corn	560:563	arg1	RS					583:584	RS	583:584	RS	583:584	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	2	5	theme	corn	560:563	arg1	starch					575:580	4, 8, and 12% corn resistant starch	546:580	4, 8, and 12% corn resistant starch (RS) (identified as 4%RS	546:605	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	7	6	theme	relative	1170:1177	arg1	expressions					1184:1194	the relative mRNA expressions	1166:1194	the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05)	1166:1404	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	8	7	theme	Microbiota	1407:1416	arg1	analysis					1418:1425	Microbiota analysis	1407:1425	Microbiota analysis	1407:1425	Microbiota analysis revealed that birds fed diets supplementation with 8 and 12% RS decreased the abundance of cecal Firmicutes by 23.08 and 20.47% and increased the proportion of Bacteroidetes by 24.33 and 21.92%, respectively, compared with the NC group (P < 0.05).
33142500	9	8	theme	abdominal	1866:1874	arg1	fat					1876:1878	the lower abdominal fat	1856:1878	the lower abdominal fat phenotype	1856:1888	In addition, correlation analysis revealed that many Firmicutes members had highly positive relationship with blood lipid levels and fat storage capacity, which might contribute to the lower abdominal fat phenotype.
33142500	6	9	theme	fatty	1044:1048	arg1	acid					1050:1053	nonestesterified fatty acid	1027:1053	nonestesterified fatty acid	1027:1053	The birds from 8%RS and 12%RS groups exhibited lower concentrations of blood triglyceride and nonestesterified fatty acid than those in the NC and CS groups (P < 0.05).
33142500	7	10	theme	proliferator-activated	1210:1231	arg1	gamma					1242:1246	peroxisome proliferator-activated receptor gamma	1199:1246	peroxisome proliferator-activated receptor gamma	1199:1246	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	10	11	theme	abdominal	2045:2053	arg1	deposition					2059:2068	abdominal fat deposition	2045:2068	abdominal fat deposition of birds during the starter phase	2045:2102	Overall, broilers receiving diets containing a higher concentration of RS harbor less Firmicutes, which decreased liver fatty acid synthesis and suppress abdominal fat deposition of birds during the starter phase.
33142500	6	12	theme	%	949:949	arg1	RS					950:951	8%RS	948:951	8%RS	948:951	The birds from 8%RS and 12%RS groups exhibited lower concentrations of blood triglyceride and nonestesterified fatty acid than those in the NC and CS groups (P < 0.05).
33142500	8	13	theme	%	1486:1486	arg1	RS					1488:1489	8 and 12% RS	1478:1489	8 and 12% RS	1478:1489	Microbiota analysis revealed that birds fed diets supplementation with 8 and 12% RS decreased the abundance of cecal Firmicutes by 23.08 and 20.47% and increased the proportion of Bacteroidetes by 24.33 and 21.92%, respectively, compared with the NC group (P < 0.05).
33142500	1	14	theme	lipid	192:196	arg1	metabolism					198:207	lipid metabolism	192:207	lipid metabolism of broilers	192:219	This study investigated the effects of dietary corn-resistant starch on lipid metabolism of broilers and its potential relationship with cecal microbiota modulation.
33142500	7	15	from	expressions	1184:1194	arg1	liver					1319:1323	liver	1319:1323	liver	1319:1323	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	7	15	from	expressions	1184:1194	arg1	tissue					1388:1393	abdominal adipose tissue	1370:1393	abdominal adipose tissue (P < 0.05)	1370:1404	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	7	15	from	expressions	1184:1194	arg1	P < 0.05					1396:1403	P < 0.05	1396:1403	P < 0.05	1396:1403	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	7	16	theme	%	1151:1151	arg1	RS					1153:1154	12% RS	1149:1154	12% RS	1149:1154	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	6	17	theme	blood	1004:1008	arg1	triglyceride					1010:1021	blood triglyceride	1004:1021	blood triglyceride	1004:1021	The birds from 8%RS and 12%RS groups exhibited lower concentrations of blood triglyceride and nonestesterified fatty acid than those in the NC and CS groups (P < 0.05).
33142500	2	18	theme	broilers	333:340	arg1	total					288:292	A total	286:292	A total of three hundred twenty 1-day-old male broilers	286:340	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	5	19	theme	abdominal	755:763	arg1	lower					785:789	lower	785:789	lower	785:789	The results showed that the abdominal fat percentage were lower in birds from 8%RS and 12%RS groups (0.75 and 0.58%, respectively) than those from NC and CS groups (1.20 and 1.28%, respectively; P < 0.05).
33142500	5	19	theme	abdominal	755:763	arg1	percentage					769:778	the abdominal fat percentage	751:778	the abdominal fat percentage	751:778	The results showed that the abdominal fat percentage were lower in birds from 8%RS and 12%RS groups (0.75 and 0.58%, respectively) than those from NC and CS groups (1.20 and 1.28%, respectively; P < 0.05).
33142500	10	20	theme	birds	2073:2077	arg1	deposition					2059:2068	abdominal fat deposition	2045:2068	abdominal fat deposition of birds during the starter phase	2045:2102	Overall, broilers receiving diets containing a higher concentration of RS harbor less Firmicutes, which decreased liver fatty acid synthesis and suppress abdominal fat deposition of birds during the starter phase.
33142500	11	21	theme	profound	2130:2137	arg1	understanding					2139:2151	a profound understanding	2128:2151	a profound understanding about the relationship between gut microbial composition and lipid metabolism in broilers	2128:2241	These findings provide a profound understanding about the relationship between gut microbial composition and lipid metabolism in broilers.
33142500	9	22	theme	correlation	1688:1698	arg1	analysis					1700:1707	correlation analysis	1688:1707	correlation analysis	1688:1707	In addition, correlation analysis revealed that many Firmicutes members had highly positive relationship with blood lipid levels and fat storage capacity, which might contribute to the lower abdominal fat phenotype.
33142500	5	23	theme	CS	881:882	arg1	%					905:905	1.20 and 1.28%	892:905	1.20 and 1.28%	892:905	The results showed that the abdominal fat percentage were lower in birds from 8%RS and 12%RS groups (0.75 and 0.58%, respectively) than those from NC and CS groups (1.20 and 1.28%, respectively; P < 0.05).
33142500	5	23	theme	CS	881:882	arg1	groups					884:889	NC and CS groups	874:889	NC and CS groups (1.20 and 1.28%, respectively; P < 0.05)	874:930	The results showed that the abdominal fat percentage were lower in birds from 8%RS and 12%RS groups (0.75 and 0.58%, respectively) than those from NC and CS groups (1.20 and 1.28%, respectively; P < 0.05).
33142500	2	24	theme	1-day-old	318:326	arg1	broilers					333:340	three hundred twenty 1-day-old male broilers	297:340	three hundred twenty 1-day-old male broilers	297:340	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	11	25	from	metabolism	2220:2229	arg1	broilers					2234:2241	broilers	2234:2241	broilers	2234:2241	These findings provide a profound understanding about the relationship between gut microbial composition and lipid metabolism in broilers.
33142500	10	26	theme	fatty	2011:2015	arg1	synthesis					2022:2030	liver fatty acid synthesis	2005:2030	liver fatty acid synthesis	2005:2030	Overall, broilers receiving diets containing a higher concentration of RS harbor less Firmicutes, which decreased liver fatty acid synthesis and suppress abdominal fat deposition of birds during the starter phase.
33142500	6	27	theme	CS	1080:1081	arg1	groups					1083:1088	the NC and CS groups	1069:1088	the NC and CS groups (P < 0.05)	1069:1099	The birds from 8%RS and 12%RS groups exhibited lower concentrations of blood triglyceride and nonestesterified fatty acid than those in the NC and CS groups (P < 0.05).
33142500	6	27	theme	CS	1080:1081	arg1	P < 0.05					1091:1098	P < 0.05	1091:1098	P < 0.05	1091:1098	The birds from 8%RS and 12%RS groups exhibited lower concentrations of blood triglyceride and nonestesterified fatty acid than those in the NC and CS groups (P < 0.05).
33142500	8	28	theme	cecal	1518:1522	arg1	Firmicutes					1524:1533	cecal Firmicutes	1518:1533	cecal Firmicutes	1518:1533	Microbiota analysis revealed that birds fed diets supplementation with 8 and 12% RS decreased the abundance of cecal Firmicutes by 23.08 and 20.47% and increased the proportion of Bacteroidetes by 24.33 and 21.92%, respectively, compared with the NC group (P < 0.05).
33142500	1	29	theme	potential	229:237	arg1	relationship					239:250	its potential relationship	225:250	its potential relationship with cecal microbiota modulation	225:283	This study investigated the effects of dietary corn-resistant starch on lipid metabolism of broilers and its potential relationship with cecal microbiota modulation.
33142500	2	30	dep	starch	575:580	arg1	%					558:558	%	558:558	%	558:558	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	6	31	theme	lower	980:984	arg1	concentrations					986:999	lower concentrations	980:999	lower concentrations of blood triglyceride and nonestesterified fatty acid	980:1053	The birds from 8%RS and 12%RS groups exhibited lower concentrations of blood triglyceride and nonestesterified fatty acid than those in the NC and CS groups (P < 0.05).
33142500	11	32	theme	gut	2184:2186	arg1	composition					2198:2208	gut microbial composition	2184:2208	gut microbial composition	2184:2208	These findings provide a profound understanding about the relationship between gut microbial composition and lipid metabolism in broilers.
33142500	5	33	dep	groups	820:825	arg1	%					841:841	0.75 and 0.58%	828:841	0.75 and 0.58%	828:841	The results showed that the abdominal fat percentage were lower in birds from 8%RS and 12%RS groups (0.75 and 0.58%, respectively) than those from NC and CS groups (1.20 and 1.28%, respectively; P < 0.05).
33142500	6	34	theme	NC	1073:1074	arg1	groups					1083:1088	the NC and CS groups	1069:1088	the NC and CS groups (P < 0.05)	1069:1099	The birds from 8%RS and 12%RS groups exhibited lower concentrations of blood triglyceride and nonestesterified fatty acid than those in the NC and CS groups (P < 0.05).
33142500	6	34	theme	NC	1073:1074	arg1	P < 0.05					1091:1098	P < 0.05	1091:1098	P < 0.05	1091:1098	The birds from 8%RS and 12%RS groups exhibited lower concentrations of blood triglyceride and nonestesterified fatty acid than those in the NC and CS groups (P < 0.05).
33142500	10	35	theme	liver	2005:2009	arg1	synthesis					2022:2030	liver fatty acid synthesis	2005:2030	liver fatty acid synthesis	2005:2030	Overall, broilers receiving diets containing a higher concentration of RS harbor less Firmicutes, which decreased liver fatty acid synthesis and suppress abdominal fat deposition of birds during the starter phase.
33142500	7	36	theme	adipose	1380:1386	arg1	tissue					1388:1393	abdominal adipose tissue	1370:1393	abdominal adipose tissue (P < 0.05)	1370:1404	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	7	36	theme	adipose	1380:1386	arg1	P < 0.05					1396:1403	P < 0.05	1396:1403	P < 0.05	1396:1403	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	8	37	theme	Firmicutes	1524:1533	arg1	abundance					1505:1513	the abundance	1501:1513	the abundance of cecal Firmicutes	1501:1533	Microbiota analysis revealed that birds fed diets supplementation with 8 and 12% RS decreased the abundance of cecal Firmicutes by 23.08 and 20.47% and increased the proportion of Bacteroidetes by 24.33 and 21.92%, respectively, compared with the NC group (P < 0.05).
33142500	5	38	theme	NC	874:875	arg1	%					905:905	1.20 and 1.28%	892:905	1.20 and 1.28%	892:905	The results showed that the abdominal fat percentage were lower in birds from 8%RS and 12%RS groups (0.75 and 0.58%, respectively) than those from NC and CS groups (1.20 and 1.28%, respectively; P < 0.05).
33142500	5	38	theme	NC	874:875	arg1	groups					884:889	NC and CS groups	874:889	NC and CS groups (1.20 and 1.28%, respectively; P < 0.05)	874:930	The results showed that the abdominal fat percentage were lower in birds from 8%RS and 12%RS groups (0.75 and 0.58%, respectively) than those from NC and CS groups (1.20 and 1.28%, respectively; P < 0.05).
33142500	5	39	theme	8	805:805	arg1	%					806:806	%	806:806	%	806:806	The results showed that the abdominal fat percentage were lower in birds from 8%RS and 12%RS groups (0.75 and 0.58%, respectively) than those from NC and CS groups (1.20 and 1.28%, respectively; P < 0.05).
33142500	6	40	theme	%	959:959	arg1	RS					960:961	12%RS	957:961	12%RS	957:961	The birds from 8%RS and 12%RS groups exhibited lower concentrations of blood triglyceride and nonestesterified fatty acid than those in the NC and CS groups (P < 0.05).
33142500	11	41	theme	lipid	2214:2218	arg1	metabolism					2220:2229	lipid metabolism	2214:2229	lipid metabolism	2214:2229	These findings provide a profound understanding about the relationship between gut microbial composition and lipid metabolism in broilers.
33142500	9	42	contain	had	1747:1749	arg1	members					1739:1745	many Firmicutes members	1723:1745	many Firmicutes members	1723:1745	In addition, correlation analysis revealed that many Firmicutes members had highly positive relationship with blood lipid levels and fat storage capacity, which might contribute to the lower abdominal fat phenotype.
33142500	9	42	contain	had	1747:1749	arg2	relationship					1767:1778	highly positive relationship	1751:1778	highly positive relationship	1751:1778	In addition, correlation analysis revealed that many Firmicutes members had highly positive relationship with blood lipid levels and fat storage capacity, which might contribute to the lower abdominal fat phenotype.
33142500	1	43	theme	dietary	159:165	arg1	starch					182:187	dietary corn-resistant starch	159:187	dietary corn-resistant starch	159:187	This study investigated the effects of dietary corn-resistant starch on lipid metabolism of broilers and its potential relationship with cecal microbiota modulation.
33142500	7	44	theme	glycerol-3-phosphate	1330:1349	arg1	acyltransferase					1351:1365	glycerol-3-phosphate acyltransferase	1330:1365	glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05)	1330:1404	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	2	45	theme	%	620:620	arg1	RS					621:622	12%RS	618:622	12%RS	618:622	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	2	46	with	diet	419:422	arg1	CS					489:490	CS	489:490	CS	489:490	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	2	46	with	diet	419:422	arg1	starch					481:486	20% corn starch	472:486	20% corn starch (CS)	472:491	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	1	47	theme	starch	182:187	arg1	relationship					239:250	its potential relationship	225:250	its potential relationship with cecal microbiota modulation	225:283	This study investigated the effects of dietary corn-resistant starch on lipid metabolism of broilers and its potential relationship with cecal microbiota modulation.
33142500	1	47	theme	starch	182:187	arg1	effects					148:154	the effects	144:154	the effects of dietary corn-resistant starch on lipid metabolism of broilers	144:219	This study investigated the effects of dietary corn-resistant starch on lipid metabolism of broilers and its potential relationship with cecal microbiota modulation.
33142500	10	48	theme	RS	1962:1963	arg1	concentration					1945:1957	a higher concentration	1936:1957	a higher concentration of RS	1936:1963	Overall, broilers receiving diets containing a higher concentration of RS harbor less Firmicutes, which decreased liver fatty acid synthesis and suppress abdominal fat deposition of birds during the starter phase.
33142500	2	49	dep	treatments	380:389	arg1	supplementation					451:465	1 corn-soybean-based diet supplementation	425:465	1 corn-soybean-based diet supplementation with 20% corn starch (CS)	425:491	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	2	49	dep	treatments	380:389	arg1	diet					419:422	1 normal corn-soybean (NC) diet	392:422	1 normal corn-soybean (NC) diet	392:422	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	2	49	dep	treatments	380:389	arg1	treatments					380:389	5 dietary treatments	370:389	5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively)	370:637	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	2	49	dep	treatments	380:389	arg1	diets					519:523	3 corn-soybean-based diets	498:523	3 corn-soybean-based diets	498:523	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	0	50	theme	cecal	102:106	arg1	Firmicutes					108:117	the reduced cecal Firmicutes	90:117	the reduced cecal Firmicutes	90:117	Dietary corn-resistant starch suppresses broiler abdominal fat deposition associated with the reduced cecal Firmicutes.
33142500	6	51	theme	RS	950:951	arg1	groups					963:968	8%RS and 12%RS groups	948:968	8%RS and 12%RS groups	948:968	The birds from 8%RS and 12%RS groups exhibited lower concentrations of blood triglyceride and nonestesterified fatty acid than those in the NC and CS groups (P < 0.05).
33142500	5	52	theme	RS	817:818	arg1	groups					820:825	8%RS and 12%RS groups	805:825	8%RS and 12%RS groups (0.75 and 0.58%, respectively)	805:856	The results showed that the abdominal fat percentage were lower in birds from 8%RS and 12%RS groups (0.75 and 0.58%, respectively) than those from NC and CS groups (1.20 and 1.28%, respectively; P < 0.05).
33142500	9	53	theme	positive	1758:1765	arg1	relationship					1767:1778	highly positive relationship	1751:1778	highly positive relationship	1751:1778	In addition, correlation analysis revealed that many Firmicutes members had highly positive relationship with blood lipid levels and fat storage capacity, which might contribute to the lower abdominal fat phenotype.
33142500	2	54	theme	NC	415:416	arg1	diet					419:422	1 normal corn-soybean (NC) diet	392:422	1 normal corn-soybean (NC) diet	392:422	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	2	54	theme	NC	415:416	arg1	treatments					380:389	5 dietary treatments	370:389	5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively)	370:637	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	7	55	with	supplementation	1128:1142	arg1	RS					1153:1154	12% RS	1149:1154	12% RS	1149:1154	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	1	56	with	effects	148:154	arg1	modulation					274:283	cecal microbiota modulation	257:283	cecal microbiota modulation	257:283	This study investigated the effects of dietary corn-resistant starch on lipid metabolism of broilers and its potential relationship with cecal microbiota modulation.
33142500	8	57	theme	Bacteroidetes	1587:1599	arg1	proportion					1573:1582	the proportion	1569:1582	the proportion of Bacteroidetes	1569:1599	Microbiota analysis revealed that birds fed diets supplementation with 8 and 12% RS decreased the abundance of cecal Firmicutes by 23.08 and 20.47% and increased the proportion of Bacteroidetes by 24.33 and 21.92%, respectively, compared with the NC group (P < 0.05).
33142500	2	58	theme	corn-soybean	401:412	arg1	diet					419:422	1 normal corn-soybean (NC) diet	392:422	1 normal corn-soybean (NC) diet	392:422	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	2	58	theme	corn-soybean	401:412	arg1	treatments					380:389	5 dietary treatments	370:389	5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively)	370:637	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	0	59	theme	Dietary	0:6	arg1	starch					23:28	Dietary corn-resistant starch	0:28	Dietary corn-resistant starch	0:28	Dietary corn-resistant starch suppresses broiler abdominal fat deposition associated with the reduced cecal Firmicutes.
33142500	2	60	with	diets	519:523	arg1	CS					489:490	CS	489:490	CS	489:490	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	2	60	with	diets	519:523	arg1	starch					481:486	20% corn starch	472:486	20% corn starch (CS)	472:491	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	5	61	theme	RS	807:808	arg1	groups					820:825	8%RS and 12%RS groups	805:825	8%RS and 12%RS groups (0.75 and 0.58%, respectively)	805:856	The results showed that the abdominal fat percentage were lower in birds from 8%RS and 12%RS groups (0.75 and 0.58%, respectively) than those from NC and CS groups (1.20 and 1.28%, respectively; P < 0.05).
33142500	2	62	theme	diet	446:449	arg1	supplementation					451:465	1 corn-soybean-based diet supplementation	425:465	1 corn-soybean-based diet supplementation with 20% corn starch (CS)	425:491	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	2	62	theme	diet	446:449	arg1	treatments					380:389	5 dietary treatments	370:389	5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively)	370:637	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	9	63	theme	lipid	1791:1795	arg1	levels					1797:1802	blood lipid levels	1785:1802	blood lipid levels	1785:1802	In addition, correlation analysis revealed that many Firmicutes members had highly positive relationship with blood lipid levels and fat storage capacity, which might contribute to the lower abdominal fat phenotype.
33142500	2	64	dep	diet	419:422	arg1	supplementation					525:539	supplementation	525:539	supplementation	525:539	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	0	65	theme	broiler	41:47	arg1	deposition					63:72	broiler abdominal fat deposition	41:72	broiler abdominal fat deposition associated with the reduced cecal Firmicutes	41:117	Dietary corn-resistant starch suppresses broiler abdominal fat deposition associated with the reduced cecal Firmicutes.
33142500	7	66	theme	synthase	1279:1286	arg1	expressions					1184:1194	the relative mRNA expressions	1166:1194	the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05)	1166:1404	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	2	67	theme	%	474:474	arg1	CS					489:490	CS	489:490	CS	489:490	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	2	67	theme	%	474:474	arg1	starch					481:486	20% corn starch	472:486	20% corn starch (CS)	472:491	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	9	68	theme	storage	1812:1818	arg1	capacity					1820:1827	fat storage capacity	1808:1827	fat storage capacity	1808:1827	In addition, correlation analysis revealed that many Firmicutes members had highly positive relationship with blood lipid levels and fat storage capacity, which might contribute to the lower abdominal fat phenotype.
33142500	0	69	theme	fat	59:61	arg1	deposition					63:72	broiler abdominal fat deposition	41:72	broiler abdominal fat deposition associated with the reduced cecal Firmicutes	41:117	Dietary corn-resistant starch suppresses broiler abdominal fat deposition associated with the reduced cecal Firmicutes.
33142500	2	70	with	treatments	380:389	arg1	RS					621:622	12%RS	618:622	12%RS	618:622	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	2	70	with	treatments	380:389	arg1	RS					610:611	8%RS	608:611	8%RS	608:611	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	2	70	with	treatments	380:389	arg1	starch					575:580	4, 8, and 12% corn resistant starch	546:580	4, 8, and 12% corn resistant starch (RS) (identified as 4%RS	546:605	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	2	70	with	treatments	380:389	arg1	RS					583:584	RS	583:584	RS	583:584	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	11	71	theme	microbial	2188:2196	arg1	composition					2198:2208	gut microbial composition	2184:2208	gut microbial composition	2184:2208	These findings provide a profound understanding about the relationship between gut microbial composition and lipid metabolism in broilers.
33142500	7	72	theme	citrate-lyase	1253:1265	arg1	expressions					1184:1194	the relative mRNA expressions	1166:1194	the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05)	1166:1404	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	9	73	dep	had	1747:1749	arg1	contribute					1842:1851	contribute	1842:1851	might contribute to the lower abdominal fat phenotype	1836:1888	In addition, correlation analysis revealed that many Firmicutes members had highly positive relationship with blood lipid levels and fat storage capacity, which might contribute to the lower abdominal fat phenotype.
33142500	1	74	theme	cecal	257:261	arg1	modulation					274:283	cecal microbiota modulation	257:283	cecal microbiota modulation	257:283	This study investigated the effects of dietary corn-resistant starch on lipid metabolism of broilers and its potential relationship with cecal microbiota modulation.
33142500	1	75	with	relationship	239:250	arg1	modulation					274:283	cecal microbiota modulation	257:283	cecal microbiota modulation	257:283	This study investigated the effects of dietary corn-resistant starch on lipid metabolism of broilers and its potential relationship with cecal microbiota modulation.
33142500	7	76	theme	receptor	1233:1240	arg1	gamma					1242:1246	peroxisome proliferator-activated receptor gamma	1199:1246	peroxisome proliferator-activated receptor gamma	1199:1246	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	2	77	theme	resistant	565:573	arg1	RS					583:584	RS	583:584	RS	583:584	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	2	77	theme	resistant	565:573	arg1	starch					575:580	4, 8, and 12% corn resistant starch	546:580	4, 8, and 12% corn resistant starch (RS) (identified as 4%RS	546:605	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	6	78	theme	acid	1050:1053	arg1	concentrations					986:999	lower concentrations	980:999	lower concentrations of blood triglyceride and nonestesterified fatty acid	980:1053	The birds from 8%RS and 12%RS groups exhibited lower concentrations of blood triglyceride and nonestesterified fatty acid than those in the NC and CS groups (P < 0.05).
33142500	9	79	theme	Firmicutes	1728:1737	arg1	members					1739:1745	many Firmicutes members	1723:1745	many Firmicutes members	1723:1745	In addition, correlation analysis revealed that many Firmicutes members had highly positive relationship with blood lipid levels and fat storage capacity, which might contribute to the lower abdominal fat phenotype.
33142500	7	80	theme	peroxisome	1199:1208	arg1	gamma					1242:1246	peroxisome proliferator-activated receptor gamma	1199:1246	peroxisome proliferator-activated receptor gamma	1199:1246	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	7	81	theme	mRNA	1179:1182	arg1	expressions					1184:1194	the relative mRNA expressions	1166:1194	the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05)	1166:1404	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	9	82	theme	fat	1876:1878	arg1	phenotype					1880:1888	the lower abdominal fat phenotype	1856:1888	the lower abdominal fat phenotype	1856:1888	In addition, correlation analysis revealed that many Firmicutes members had highly positive relationship with blood lipid levels and fat storage capacity, which might contribute to the lower abdominal fat phenotype.
33142500	6	83	theme	nonestesterified	1027:1042	arg1	acid					1050:1053	nonestesterified fatty acid	1027:1053	nonestesterified fatty acid	1027:1053	The birds from 8%RS and 12%RS groups exhibited lower concentrations of blood triglyceride and nonestesterified fatty acid than those in the NC and CS groups (P < 0.05).
33142500	6	84	theme	8	948:948	arg1	%					949:949	%	949:949	%	949:949	The birds from 8%RS and 12%RS groups exhibited lower concentrations of blood triglyceride and nonestesterified fatty acid than those in the NC and CS groups (P < 0.05).
33142500	6	85	theme	triglyceride	1010:1021	arg1	concentrations					986:999	lower concentrations	980:999	lower concentrations of blood triglyceride and nonestesterified fatty acid	980:1053	The birds from 8%RS and 12%RS groups exhibited lower concentrations of blood triglyceride and nonestesterified fatty acid than those in the NC and CS groups (P < 0.05).
33142500	7	86	dep	diets	1122:1126	arg1	decreased					1156:1164	decreased	1156:1164	decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05)	1156:1404	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	7	87	theme	fatty	1268:1272	arg1	synthase					1279:1286	fatty acid synthase	1268:1286	fatty acid synthase	1268:1286	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	8	88	theme	NC	1654:1655	arg1	group					1657:1661	the NC group	1650:1661	the NC group (P < 0.05)	1650:1672	Microbiota analysis revealed that birds fed diets supplementation with 8 and 12% RS decreased the abundance of cecal Firmicutes by 23.08 and 20.47% and increased the proportion of Bacteroidetes by 24.33 and 21.92%, respectively, compared with the NC group (P < 0.05).
33142500	8	88	theme	NC	1654:1655	arg1	P < 0.05					1664:1671	P < 0.05	1664:1671	P < 0.05	1664:1671	Microbiota analysis revealed that birds fed diets supplementation with 8 and 12% RS decreased the abundance of cecal Firmicutes by 23.08 and 20.47% and increased the proportion of Bacteroidetes by 24.33 and 21.92%, respectively, compared with the NC group (P < 0.05).
33142500	2	89	theme	male	328:331	arg1	broilers					333:340	three hundred twenty 1-day-old male broilers	297:340	three hundred twenty 1-day-old male broilers	297:340	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	1	90	theme	broilers	212:219	arg1	metabolism					198:207	lipid metabolism	192:207	lipid metabolism of broilers	192:219	This study investigated the effects of dietary corn-resistant starch on lipid metabolism of broilers and its potential relationship with cecal microbiota modulation.
33142500	7	91	theme	12	1149:1150	arg1	%					1151:1151	%	1151:1151	%	1151:1151	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	2	92	dep	%	558:558	arg1	8					549:549	8	549:549	8	549:549	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	2	92	dep	%	558:558	arg1	12					556:557	12	556:557	12	556:557	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	10	93	theme	fat	2055:2057	arg1	deposition					2059:2068	abdominal fat deposition	2045:2068	abdominal fat deposition of birds during the starter phase	2045:2102	Overall, broilers receiving diets containing a higher concentration of RS harbor less Firmicutes, which decreased liver fatty acid synthesis and suppress abdominal fat deposition of birds during the starter phase.
33142500	2	94	theme	12	618:619	arg1	%					620:620	%	620:620	%	620:620	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	10	95	contain	containing	1925:1934	arg2	concentration					1945:1957	a higher concentration	1936:1957	a higher concentration of RS	1936:1963	Overall, broilers receiving diets containing a higher concentration of RS harbor less Firmicutes, which decreased liver fatty acid synthesis and suppress abdominal fat deposition of birds during the starter phase.
33142500	10	95	contain	containing	1925:1934	arg1	diets					1919:1923	diets	1919:1923	diets containing a higher concentration of RS	1919:1963	Overall, broilers receiving diets containing a higher concentration of RS harbor less Firmicutes, which decreased liver fatty acid synthesis and suppress abdominal fat deposition of birds during the starter phase.
33142500	7	96	theme	abdominal	1370:1378	arg1	tissue					1388:1393	abdominal adipose tissue	1370:1393	abdominal adipose tissue (P < 0.05)	1370:1404	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	7	96	theme	abdominal	1370:1378	arg1	P < 0.05					1396:1403	P < 0.05	1396:1403	P < 0.05	1396:1403	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	10	97	theme	starter	2090:2096	arg1	phase					2098:2102	the starter phase	2086:2102	the starter phase	2086:2102	Overall, broilers receiving diets containing a higher concentration of RS harbor less Firmicutes, which decreased liver fatty acid synthesis and suppress abdominal fat deposition of birds during the starter phase.
33142500	8	98	dep	diets	1451:1455	arg1	decreased					1491:1499	decreased	1491:1499	decreased the abundance of cecal Firmicutes by 23.08 and 20.47%	1491:1553	Microbiota analysis revealed that birds fed diets supplementation with 8 and 12% RS decreased the abundance of cecal Firmicutes by 23.08 and 20.47% and increased the proportion of Bacteroidetes by 24.33 and 21.92%, respectively, compared with the NC group (P < 0.05).
33142500	8	98	dep	diets	1451:1455	arg1	increased					1559:1567	increased	1559:1567	increased the proportion of Bacteroidetes by 24.33 and 21.92%, respectively	1559:1633	Microbiota analysis revealed that birds fed diets supplementation with 8 and 12% RS decreased the abundance of cecal Firmicutes by 23.08 and 20.47% and increased the proportion of Bacteroidetes by 24.33 and 21.92%, respectively, compared with the NC group (P < 0.05).
33142500	1	99	from	relationship	239:250	arg1	metabolism					198:207	lipid metabolism	192:207	lipid metabolism of broilers	192:219	This study investigated the effects of dietary corn-resistant starch on lipid metabolism of broilers and its potential relationship with cecal microbiota modulation.
33142500	2	100	with	supplementation	451:465	arg1	CS					489:490	CS	489:490	CS	489:490	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	2	100	with	supplementation	451:465	arg1	starch					481:486	20% corn starch	472:486	20% corn starch (CS)	472:491	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	7	101	theme	acyltransferase	1351:1365	arg1	expressions					1184:1194	the relative mRNA expressions	1166:1194	the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05)	1166:1404	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	6	102	theme	RS	960:961	arg1	groups					963:968	8%RS and 12%RS groups	948:968	8%RS and 12%RS groups	948:968	The birds from 8%RS and 12%RS groups exhibited lower concentrations of blood triglyceride and nonestesterified fatty acid than those in the NC and CS groups (P < 0.05).
33142500	10	103	theme	higher	1938:1943	arg1	concentration					1945:1957	a higher concentration	1936:1957	a higher concentration of RS	1936:1963	Overall, broilers receiving diets containing a higher concentration of RS harbor less Firmicutes, which decreased liver fatty acid synthesis and suppress abdominal fat deposition of birds during the starter phase.
33142500	5	104	theme	fat	765:767	arg1	lower					785:789	lower	785:789	lower	785:789	The results showed that the abdominal fat percentage were lower in birds from 8%RS and 12%RS groups (0.75 and 0.58%, respectively) than those from NC and CS groups (1.20 and 1.28%, respectively; P < 0.05).
33142500	5	104	theme	fat	765:767	arg1	percentage					769:778	the abdominal fat percentage	751:778	the abdominal fat percentage	751:778	The results showed that the abdominal fat percentage were lower in birds from 8%RS and 12%RS groups (0.75 and 0.58%, respectively) than those from NC and CS groups (1.20 and 1.28%, respectively; P < 0.05).
33142500	6	105	theme	12	957:958	arg1	%					959:959	%	959:959	%	959:959	The birds from 8%RS and 12%RS groups exhibited lower concentrations of blood triglyceride and nonestesterified fatty acid than those in the NC and CS groups (P < 0.05).
33142500	9	106	theme	many	1723:1726	arg1	members					1739:1745	many Firmicutes members	1723:1745	many Firmicutes members	1723:1745	In addition, correlation analysis revealed that many Firmicutes members had highly positive relationship with blood lipid levels and fat storage capacity, which might contribute to the lower abdominal fat phenotype.
33142500	0	107	theme	reduced	94:100	arg1	Firmicutes					108:117	the reduced cecal Firmicutes	90:117	the reduced cecal Firmicutes	90:117	Dietary corn-resistant starch suppresses broiler abdominal fat deposition associated with the reduced cecal Firmicutes.
33142500	1	108	theme	corn-resistant	167:180	arg1	starch					182:187	dietary corn-resistant starch	159:187	dietary corn-resistant starch	159:187	This study investigated the effects of dietary corn-resistant starch on lipid metabolism of broilers and its potential relationship with cecal microbiota modulation.
33142500	2	109	theme	%	603:603	arg1	RS					604:605	4%RS	602:605	4%RS	602:605	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	6	110	from	groups	963:968	arg1	birds					937:941	The birds	933:941	The birds from 8%RS and 12%RS groups	933:968	The birds from 8%RS and 12%RS groups exhibited lower concentrations of blood triglyceride and nonestesterified fatty acid than those in the NC and CS groups (P < 0.05).
33142500	7	111	from	tissue	1388:1393	arg1	expressions					1184:1194	the relative mRNA expressions	1166:1194	the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05)	1166:1404	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	5	112	theme	%	816:816	arg1	groups					820:825	8%RS and 12%RS groups	805:825	8%RS and 12%RS groups (0.75 and 0.58%, respectively)	805:856	The results showed that the abdominal fat percentage were lower in birds from 8%RS and 12%RS groups (0.75 and 0.58%, respectively) than those from NC and CS groups (1.20 and 1.28%, respectively; P < 0.05).
33142500	2	113	theme	corn-soybean-based	500:517	arg1	diets					519:523	3 corn-soybean-based diets	498:523	3 corn-soybean-based diets	498:523	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	3	114	contain	had	651:653	arg2	8					655:655	8	655:655	8	655:655	Each group had 8 replicates with 8 broilers per replicate.
33142500	3	114	contain	had	651:653	arg1	group					645:649	Each group	640:649	Each group	640:649	Each group had 8 replicates with 8 broilers per replicate.
33142500	3	115	dep	8	655:655	arg1	replicates					657:666	replicates	657:666	replicates	657:666	Each group had 8 replicates with 8 broilers per replicate.
33142500	2	116	theme	%	609:609	arg1	RS					610:611	8%RS	608:611	8%RS	608:611	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	11	117	from	composition	2198:2208	arg1	broilers					2234:2241	broilers	2234:2241	broilers	2234:2241	These findings provide a profound understanding about the relationship between gut microbial composition and lipid metabolism in broilers.
33142500	0	118	theme	corn-resistant	8:21	arg1	starch					23:28	Dietary corn-resistant starch	0:28	Dietary corn-resistant starch	0:28	Dietary corn-resistant starch suppresses broiler abdominal fat deposition associated with the reduced cecal Firmicutes.
33142500	1	119	from	effects	148:154	arg1	metabolism					198:207	lipid metabolism	192:207	lipid metabolism of broilers	192:219	This study investigated the effects of dietary corn-resistant starch on lipid metabolism of broilers and its potential relationship with cecal microbiota modulation.
33142500	5	120	theme	%	806:806	arg1	RS					807:808	8%RS	805:808	8%RS	805:808	The results showed that the abdominal fat percentage were lower in birds from 8%RS and 12%RS groups (0.75 and 0.58%, respectively) than those from NC and CS groups (1.20 and 1.28%, respectively; P < 0.05).
33142500	9	121	theme	blood	1785:1789	arg1	levels					1797:1802	blood lipid levels	1785:1802	blood lipid levels	1785:1802	In addition, correlation analysis revealed that many Firmicutes members had highly positive relationship with blood lipid levels and fat storage capacity, which might contribute to the lower abdominal fat phenotype.
33142500	2	122	theme	normal	394:399	arg1	diet					419:422	1 normal corn-soybean (NC) diet	392:422	1 normal corn-soybean (NC) diet	392:422	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	2	122	theme	normal	394:399	arg1	treatments					380:389	5 dietary treatments	370:389	5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively)	370:637	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	7	123	from	carboxylase	1304:1314	arg1	liver					1319:1323	liver	1319:1323	liver	1319:1323	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	7	123	from	carboxylase	1304:1314	arg1	tissue					1388:1393	abdominal adipose tissue	1370:1393	abdominal adipose tissue (P < 0.05)	1370:1404	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	7	123	from	carboxylase	1304:1314	arg1	P < 0.05					1396:1403	P < 0.05	1396:1403	P < 0.05	1396:1403	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	2	124	theme	corn-soybean-based	427:444	arg1	supplementation					451:465	1 corn-soybean-based diet supplementation	425:465	1 corn-soybean-based diet supplementation with 20% corn starch (CS)	425:491	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	2	124	theme	corn-soybean-based	427:444	arg1	treatments					380:389	5 dietary treatments	370:389	5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively)	370:637	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	0	125	theme	abdominal	49:57	arg1	deposition					63:72	broiler abdominal fat deposition	41:72	broiler abdominal fat deposition associated with the reduced cecal Firmicutes	41:117	Dietary corn-resistant starch suppresses broiler abdominal fat deposition associated with the reduced cecal Firmicutes.
33142500	7	126	from	liver	1319:1323	arg1	expressions					1184:1194	the relative mRNA expressions	1166:1194	the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05)	1166:1404	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	7	127	theme	acetyl-CoA	1293:1302	arg1	carboxylase					1304:1314	acetyl-CoA carboxylase	1293:1314	acetyl-CoA carboxylase	1293:1314	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	2	128	theme	corn	476:479	arg1	CS					489:490	CS	489:490	CS	489:490	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	2	128	theme	corn	476:479	arg1	starch					481:486	20% corn starch	472:486	20% corn starch (CS)	472:491	A total of three hundred twenty 1-day-old male broilers were randomly assigned into 5 dietary treatments: 1 normal corn-soybean (NC) diet, 1 corn-soybean-based diet supplementation with 20% corn starch (CS), and 3 corn-soybean-based diets supplementation with 4, 8, and 12% corn resistant starch (RS) (identified as 4%RS, 8%RS, and 12%RS, respectively).
33142500	5	129	dep	%	905:905	arg1	P < 0.05					922:929	P < 0.05	922:929	P < 0.05	922:929	The results showed that the abdominal fat percentage were lower in birds from 8%RS and 12%RS groups (0.75 and 0.58%, respectively) than those from NC and CS groups (1.20 and 1.28%, respectively; P < 0.05).
33142500	7	130	from	gamma	1242:1246	arg1	liver					1319:1323	liver	1319:1323	liver	1319:1323	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	7	130	from	gamma	1242:1246	arg1	tissue					1388:1393	abdominal adipose tissue	1370:1393	abdominal adipose tissue (P < 0.05)	1370:1404	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	7	130	from	gamma	1242:1246	arg1	P < 0.05					1396:1403	P < 0.05	1396:1403	P < 0.05	1396:1403	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	9	131	theme	fat	1808:1810	arg1	capacity					1820:1827	fat storage capacity	1808:1827	fat storage capacity	1808:1827	In addition, correlation analysis revealed that many Firmicutes members had highly positive relationship with blood lipid levels and fat storage capacity, which might contribute to the lower abdominal fat phenotype.
33142500	8	132	with	supplementation	1457:1471	arg1	RS					1488:1489	8 and 12% RS	1478:1489	8 and 12% RS	1478:1489	Microbiota analysis revealed that birds fed diets supplementation with 8 and 12% RS decreased the abundance of cecal Firmicutes by 23.08 and 20.47% and increased the proportion of Bacteroidetes by 24.33 and 21.92%, respectively, compared with the NC group (P < 0.05).
33142500	7	133	from	acyltransferase	1351:1365	arg1	liver					1319:1323	liver	1319:1323	liver	1319:1323	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	7	133	from	acyltransferase	1351:1365	arg1	tissue					1388:1393	abdominal adipose tissue	1370:1393	abdominal adipose tissue (P < 0.05)	1370:1404	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	7	133	from	acyltransferase	1351:1365	arg1	P < 0.05					1396:1403	P < 0.05	1396:1403	P < 0.05	1396:1403	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	7	134	theme	acid	1274:1277	arg1	synthase					1279:1286	fatty acid synthase	1268:1286	fatty acid synthase	1268:1286	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	7	135	from	citrate-lyase	1253:1265	arg1	liver					1319:1323	liver	1319:1323	liver	1319:1323	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	7	135	from	citrate-lyase	1253:1265	arg1	tissue					1388:1393	abdominal adipose tissue	1370:1393	abdominal adipose tissue (P < 0.05)	1370:1404	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	7	135	from	citrate-lyase	1253:1265	arg1	P < 0.05					1396:1403	P < 0.05	1396:1403	P < 0.05	1396:1403	Moreover, birds fed diets supplementation with 12% RS decreased the relative mRNA expressions of peroxisome proliferator-activated receptor gamma, ATP citrate-lyase, fatty acid synthase, and acetyl-CoA carboxylase in liver, and glycerol-3-phosphate acyltransferase in abdominal adipose tissue (P < 0.05).
33142500	5	136	from	groups	820:825	arg1	birds					794:798	birds	794:798	birds from 8%RS and 12%RS groups (0.75 and 0.58%, respectively)	794:856	The results showed that the abdominal fat percentage were lower in birds from 8%RS and 12%RS groups (0.75 and 0.58%, respectively) than those from NC and CS groups (1.20 and 1.28%, respectively; P < 0.05).
33142500	1	137	theme	microbiota	263:272	arg1	modulation					274:283	cecal microbiota modulation	257:283	cecal microbiota modulation	257:283	This study investigated the effects of dietary corn-resistant starch on lipid metabolism of broilers and its potential relationship with cecal microbiota modulation.
32041195	6	0	from	mL	1014:1015	arg1	content					985:991	protein content	977:991	protein content at approximately 445 mL	977:1015	Protein content in the fourth quartile of volume was significantly lower than that in the first three, suggesting the existence of a volume threshold for protein content at approximately 445 mL.
32041195	1	1	theme	Most	119:122	arg1	studies					124:130	Most studies	119:130	Most studies assessing the macronutrient content of human milk	119:180	Most studies assessing the macronutrient content of human milk are published retrospectively using analyzers that fail to determine sodium content and do not take into account the role of volume in milk composition.
32041195	4	2	from	lactose	631:637	arg1	mothers					667:673	40 mothers	664:673	40 mothers	664:673	Contents of protein, fat, and lactose of 102 milk samples from 40 mothers were determined using a human milk analyzer and that of sodium with a flame spectrophotometer.
32041195	0	3	theme	Nutrient	53:60	arg1	Content					62:68	Nutrient Content	53:68	Nutrient Content of Human Milk	53:82	Role of Daily Milk Volume and Period of Lactation in Nutrient Content of Human Milk: Results from a Prospective Study.
32041195	8	4	theme	preterm	1415:1421	arg1	infants					1423:1429	feeding preterm infants	1407:1429	feeding preterm infants	1407:1429	In consideration of previous findings along with our data, we suggest that extra care should be taken with fortification for feeding preterm infants when the mother's milk volume is greater than 400-450 mL.
32041195	4	5	theme	lactose	631:637	arg1	Contents					601:608	Contents	601:608	Contents of protein, fat, and lactose of 102 milk samples from 40 mothers	601:673	Contents of protein, fat, and lactose of 102 milk samples from 40 mothers were determined using a human milk analyzer and that of sodium with a flame spectrophotometer.
32041195	8	6	theme	feeding	1407:1413	arg1	infants					1423:1429	feeding preterm infants	1407:1429	feeding preterm infants	1407:1429	In consideration of previous findings along with our data, we suggest that extra care should be taken with fortification for feeding preterm infants when the mother's milk volume is greater than 400-450 mL.
32041195	0	7	theme	Human	73:77	arg1	Milk					79:82	Human Milk	73:82	Human Milk	73:82	Role of Daily Milk Volume and Period of Lactation in Nutrient Content of Human Milk: Results from a Prospective Study.
32041195	1	8	theme	volume	307:312	arg1	role					299:302	the role	295:302	the role of volume in milk composition	295:332	Most studies assessing the macronutrient content of human milk are published retrospectively using analyzers that fail to determine sodium content and do not take into account the role of volume in milk composition.
32041195	5	9	dep	along	783:787	arg1	with					789:792	with	789:792	with	789:792	Milk volumes along with clinical data were recorded.
32041195	7	10	theme	lactation	1099:1107	arg1	period					1109:1114	lactation period	1099:1114	lactation period	1099:1114	After multivariate analysis, it was found that maternal age, average volume, and lactation period remained significantly associated with protein content, maternal age remained significantly associated with fat content, and only average volume with sodium content.
32041195	6	11	theme	Protein	823:829	arg1	content					831:837	Protein content	823:837	Protein content in the fourth quartile of volume	823:870	Protein content in the fourth quartile of volume was significantly lower than that in the first three, suggesting the existence of a volume threshold for protein content at approximately 445 mL.
32041195	7	12	theme	multivariate	1024:1035	arg1	analysis					1037:1044	multivariate analysis	1024:1044	multivariate analysis	1024:1044	After multivariate analysis, it was found that maternal age, average volume, and lactation period remained significantly associated with protein content, maternal age remained significantly associated with fat content, and only average volume with sodium content.
32041195	4	13	from	fat	622:624	arg1	mothers					667:673	40 mothers	664:673	40 mothers	664:673	Contents of protein, fat, and lactose of 102 milk samples from 40 mothers were determined using a human milk analyzer and that of sodium with a flame spectrophotometer.
32041195	0	14	from	Study	112:116	arg1	Results					85:91	Results	85:91	Role of Daily Milk Volume and Period of Lactation in Nutrient Content of Human Milk: Results from a Prospective Study.	0:117	Role of Daily Milk Volume and Period of Lactation in Nutrient Content of Human Milk: Results from a Prospective Study.
32041195	1	15	theme	milk	317:320	arg1	composition					322:332	milk composition	317:332	milk composition	317:332	Most studies assessing the macronutrient content of human milk are published retrospectively using analyzers that fail to determine sodium content and do not take into account the role of volume in milk composition.
32041195	6	16	theme	volume	956:961	arg1	threshold					963:971	a volume threshold	954:971	a volume threshold for protein content at approximately 445 mL	954:1015	Protein content in the fourth quartile of volume was significantly lower than that in the first three, suggesting the existence of a volume threshold for protein content at approximately 445 mL.
32041195	4	17	theme	milk	705:708	arg1	analyzer					710:717	a human milk analyzer	697:717	a human milk analyzer	697:717	Contents of protein, fat, and lactose of 102 milk samples from 40 mothers were determined using a human milk analyzer and that of sodium with a flame spectrophotometer.
32041195	3	18	dep	prospective	540:550	arg1	monocentric					567:577	monocentric	567:577	monocentric	567:577	A prospective, longitudinal, monocentric study was undertaken.
32041195	3	18	dep	prospective	540:550	arg1	longitudinal					553:564	longitudinal	553:564	longitudinal	553:564	A prospective, longitudinal, monocentric study was undertaken.
32041195	4	19	theme	protein	613:619	arg1	Contents					601:608	Contents	601:608	Contents of protein, fat, and lactose of 102 milk samples from 40 mothers	601:673	Contents of protein, fat, and lactose of 102 milk samples from 40 mothers were determined using a human milk analyzer and that of sodium with a flame spectrophotometer.
32041195	0	20	theme	Milk	79:82	arg1	Content					62:68	Nutrient Content	53:68	Nutrient Content of Human Milk	53:82	Role of Daily Milk Volume and Period of Lactation in Nutrient Content of Human Milk: Results from a Prospective Study.
32041195	4	21	theme	human	699:703	arg1	analyzer					710:717	a human milk analyzer	697:717	a human milk analyzer	697:717	Contents of protein, fat, and lactose of 102 milk samples from 40 mothers were determined using a human milk analyzer and that of sodium with a flame spectrophotometer.
32041195	4	22	from	protein	613:619	arg1	mothers					667:673	40 mothers	664:673	40 mothers	664:673	Contents of protein, fat, and lactose of 102 milk samples from 40 mothers were determined using a human milk analyzer and that of sodium with a flame spectrophotometer.
32041195	7	23	theme	average	1246:1252	arg1	volume					1254:1259	average volume	1246:1259	average volume	1246:1259	After multivariate analysis, it was found that maternal age, average volume, and lactation period remained significantly associated with protein content, maternal age remained significantly associated with fat content, and only average volume with sodium content.
32041195	8	24	theme	milk	1449:1452	arg1	volume					1454:1459	the mother's milk volume	1436:1459	the mother's milk volume	1436:1459	In consideration of previous findings along with our data, we suggest that extra care should be taken with fortification for feeding preterm infants when the mother's milk volume is greater than 400-450 mL.
32041195	6	25	theme	protein	977:983	arg1	content					985:991	protein content	977:991	protein content at approximately 445 mL	977:1015	Protein content in the fourth quartile of volume was significantly lower than that in the first three, suggesting the existence of a volume threshold for protein content at approximately 445 mL.
32041195	1	26	theme	macronutrient	146:158	arg1	content					160:166	the macronutrient content	142:166	the macronutrient content of human milk	142:180	Most studies assessing the macronutrient content of human milk are published retrospectively using analyzers that fail to determine sodium content and do not take into account the role of volume in milk composition.
32041195	2	27	theme	human	400:404	arg1	milk					406:409	human milk	400:409	human milk	400:409	We aimed to describe macronutrient content and sodium content in human milk over time, observe any associations between them, and determine the factors associated with the evolution of milk composition.
32041195	1	28	theme	sodium	251:256	arg1	content					258:264	sodium content	251:264	sodium content	251:264	Most studies assessing the macronutrient content of human milk are published retrospectively using analyzers that fail to determine sodium content and do not take into account the role of volume in milk composition.
32041195	4	29	from	mothers	667:673	arg1	lactose					631:637	lactose	631:637	lactose of 102 milk samples from 40 mothers	631:673	Contents of protein, fat, and lactose of 102 milk samples from 40 mothers were determined using a human milk analyzer and that of sodium with a flame spectrophotometer.
32041195	4	29	from	mothers	667:673	arg1	protein					613:619	protein	613:619	protein	613:619	Contents of protein, fat, and lactose of 102 milk samples from 40 mothers were determined using a human milk analyzer and that of sodium with a flame spectrophotometer.
32041195	4	29	from	mothers	667:673	arg1	samples					651:657	102 milk samples	642:657	102 milk samples from 40 mothers	642:673	Contents of protein, fat, and lactose of 102 milk samples from 40 mothers were determined using a human milk analyzer and that of sodium with a flame spectrophotometer.
32041195	4	29	from	mothers	667:673	arg1	fat					622:624	fat	622:624	fat	622:624	Contents of protein, fat, and lactose of 102 milk samples from 40 mothers were determined using a human milk analyzer and that of sodium with a flame spectrophotometer.
32041195	0	30	theme	Period	30:35	arg1	Role					0:3	Role	0:3	Role of Daily Milk Volume and Period of Lactation in Nutrient Content of Human Milk: Results from a Prospective Study.	0:117	Role of Daily Milk Volume and Period of Lactation in Nutrient Content of Human Milk: Results from a Prospective Study.
32041195	0	31	theme	Milk	14:17	arg1	Volume					19:24	Daily Milk Volume	8:24	Daily Milk Volume	8:24	Role of Daily Milk Volume and Period of Lactation in Nutrient Content of Human Milk: Results from a Prospective Study.
32041195	8	32	theme	extra	1357:1361	arg1	care					1363:1366	extra care	1357:1366	extra care	1357:1366	In consideration of previous findings along with our data, we suggest that extra care should be taken with fortification for feeding preterm infants when the mother's milk volume is greater than 400-450 mL.
32041195	8	33	theme	findings	1311:1318	arg1	consideration					1285:1297	consideration	1285:1297	consideration of previous findings along with our data	1285:1338	In consideration of previous findings along with our data, we suggest that extra care should be taken with fortification for feeding preterm infants when the mother's milk volume is greater than 400-450 mL.
32041195	2	34	theme	sodium	382:387	arg1	content					389:395	sodium content	382:395	sodium content	382:395	We aimed to describe macronutrient content and sodium content in human milk over time, observe any associations between them, and determine the factors associated with the evolution of milk composition.
32041195	0	35	theme	Daily	8:12	arg1	Volume					19:24	Daily Milk Volume	8:24	Daily Milk Volume	8:24	Role of Daily Milk Volume and Period of Lactation in Nutrient Content of Human Milk: Results from a Prospective Study.
32041195	7	36	theme	protein	1155:1161	arg1	content					1163:1169	protein content	1155:1169	protein content	1155:1169	After multivariate analysis, it was found that maternal age, average volume, and lactation period remained significantly associated with protein content, maternal age remained significantly associated with fat content, and only average volume with sodium content.
32041195	1	37	theme	human	171:175	arg1	milk					177:180	human milk	171:180	human milk	171:180	Most studies assessing the macronutrient content of human milk are published retrospectively using analyzers that fail to determine sodium content and do not take into account the role of volume in milk composition.
32041195	4	38	theme	fat	622:624	arg1	Contents					601:608	Contents	601:608	Contents of protein, fat, and lactose of 102 milk samples from 40 mothers	601:673	Contents of protein, fat, and lactose of 102 milk samples from 40 mothers were determined using a human milk analyzer and that of sodium with a flame spectrophotometer.
32041195	0	39	theme	Prospective	100:110	arg1	Study					112:116	a Prospective Study	98:116	a Prospective Study	98:116	Role of Daily Milk Volume and Period of Lactation in Nutrient Content of Human Milk: Results from a Prospective Study.
32041195	5	40	theme	Milk	770:773	arg1	volumes					775:781	Milk volumes	770:781	Milk volumes along with clinical data	770:806	Milk volumes along with clinical data were recorded.
32041195	3	41	theme	prospective	540:550	arg1	study					579:583	A prospective, longitudinal, monocentric study	538:583	A prospective, longitudinal, monocentric study	538:583	A prospective, longitudinal, monocentric study was undertaken.
32041195	1	42	theme	milk	177:180	arg1	content					160:166	the macronutrient content	142:166	the macronutrient content of human milk	142:180	Most studies assessing the macronutrient content of human milk are published retrospectively using analyzers that fail to determine sodium content and do not take into account the role of volume in milk composition.
32041195	0	43	theme	Volume	19:24	arg1	Role					0:3	Role	0:3	Role of Daily Milk Volume and Period of Lactation in Nutrient Content of Human Milk: Results from a Prospective Study.	0:117	Role of Daily Milk Volume and Period of Lactation in Nutrient Content of Human Milk: Results from a Prospective Study.
32041195	5	44	theme	clinical	794:801	arg1	data					803:806	clinical data	794:806	Milk volumes along with clinical data	770:806	Milk volumes along with clinical data were recorded.
32041195	7	45	theme	maternal	1065:1072	arg1	age					1074:1076	maternal age	1065:1076	maternal age	1065:1076	After multivariate analysis, it was found that maternal age, average volume, and lactation period remained significantly associated with protein content, maternal age remained significantly associated with fat content, and only average volume with sodium content.
32041195	2	46	theme	macronutrient	356:368	arg1	content					370:376	macronutrient content	356:376	macronutrient content	356:376	We aimed to describe macronutrient content and sodium content in human milk over time, observe any associations between them, and determine the factors associated with the evolution of milk composition.
32041195	6	47	theme	volume	865:870	arg1	quartile					853:860	the fourth quartile	842:860	the fourth quartile of volume	842:870	Protein content in the fourth quartile of volume was significantly lower than that in the first three, suggesting the existence of a volume threshold for protein content at approximately 445 mL.
32041195	2	48	theme	composition	525:535	arg1	evolution					507:515	the evolution	503:515	the evolution of milk composition	503:535	We aimed to describe macronutrient content and sodium content in human milk over time, observe any associations between them, and determine the factors associated with the evolution of milk composition.
32041195	6	49	from	content	831:837	arg1	quartile					853:860	the fourth quartile	842:860	the fourth quartile of volume	842:870	Protein content in the fourth quartile of volume was significantly lower than that in the first three, suggesting the existence of a volume threshold for protein content at approximately 445 mL.
32041195	4	50	theme	flame	745:749	arg1	spectrophotometer					751:767	a flame spectrophotometer	743:767	a flame spectrophotometer	743:767	Contents of protein, fat, and lactose of 102 milk samples from 40 mothers were determined using a human milk analyzer and that of sodium with a flame spectrophotometer.
32041195	4	51	theme	milk	646:649	arg1	samples					651:657	102 milk samples	642:657	102 milk samples from 40 mothers	642:673	Contents of protein, fat, and lactose of 102 milk samples from 40 mothers were determined using a human milk analyzer and that of sodium with a flame spectrophotometer.
32041195	1	52	from	role	299:302	arg1	composition					322:332	milk composition	317:332	milk composition	317:332	Most studies assessing the macronutrient content of human milk are published retrospectively using analyzers that fail to determine sodium content and do not take into account the role of volume in milk composition.
32041195	2	53	theme	milk	520:523	arg1	composition					525:535	milk composition	520:535	milk composition	520:535	We aimed to describe macronutrient content and sodium content in human milk over time, observe any associations between them, and determine the factors associated with the evolution of milk composition.
32041195	7	54	dep	content	1228:1234	arg1	volume					1254:1259	average volume	1246:1259	average volume	1246:1259	After multivariate analysis, it was found that maternal age, average volume, and lactation period remained significantly associated with protein content, maternal age remained significantly associated with fat content, and only average volume with sodium content.
32041195	8	55	theme	data	1335:1338	arg1	consideration					1285:1297	consideration	1285:1297	consideration of previous findings along with our data	1285:1338	In consideration of previous findings along with our data, we suggest that extra care should be taken with fortification for feeding preterm infants when the mother's milk volume is greater than 400-450 mL.
32041195	7	56	theme	sodium	1266:1271	arg1	content					1273:1279	sodium content	1266:1279	sodium content	1266:1279	After multivariate analysis, it was found that maternal age, average volume, and lactation period remained significantly associated with protein content, maternal age remained significantly associated with fat content, and only average volume with sodium content.
32041195	8	57	theme	previous	1302:1309	arg1	findings					1311:1318	previous findings	1302:1318	previous findings along with our data	1302:1338	In consideration of previous findings along with our data, we suggest that extra care should be taken with fortification for feeding preterm infants when the mother's milk volume is greater than 400-450 mL.
32041195	7	58	theme	maternal	1172:1179	arg1	age					1181:1183	maternal age	1172:1183	maternal age	1172:1183	After multivariate analysis, it was found that maternal age, average volume, and lactation period remained significantly associated with protein content, maternal age remained significantly associated with fat content, and only average volume with sodium content.
32041195	4	59	theme	samples	651:657	arg1	lactose					631:637	lactose	631:637	lactose of 102 milk samples from 40 mothers	631:673	Contents of protein, fat, and lactose of 102 milk samples from 40 mothers were determined using a human milk analyzer and that of sodium with a flame spectrophotometer.
32041195	4	59	theme	samples	651:657	arg1	protein					613:619	protein	613:619	protein	613:619	Contents of protein, fat, and lactose of 102 milk samples from 40 mothers were determined using a human milk analyzer and that of sodium with a flame spectrophotometer.
32041195	4	59	theme	samples	651:657	arg1	fat					622:624	fat	622:624	fat	622:624	Contents of protein, fat, and lactose of 102 milk samples from 40 mothers were determined using a human milk analyzer and that of sodium with a flame spectrophotometer.
32041195	6	60	theme	threshold	963:971	arg1	existence					941:949	the existence	937:949	the existence of a volume threshold for protein content at approximately 445 mL	937:1015	Protein content in the fourth quartile of volume was significantly lower than that in the first three, suggesting the existence of a volume threshold for protein content at approximately 445 mL.
32041195	7	61	theme	fat	1224:1226	arg1	content					1228:1234	fat content	1224:1234	fat content	1224:1234	After multivariate analysis, it was found that maternal age, average volume, and lactation period remained significantly associated with protein content, maternal age remained significantly associated with fat content, and only average volume with sodium content.
32041195	0	62	theme	Lactation	40:48	arg1	Period					30:35	Period	30:35	Period of Lactation	30:48	Role of Daily Milk Volume and Period of Lactation in Nutrient Content of Human Milk: Results from a Prospective Study.
32041195	0	62	theme	Lactation	40:48	arg1	Volume					19:24	Daily Milk Volume	8:24	Daily Milk Volume	8:24	Role of Daily Milk Volume and Period of Lactation in Nutrient Content of Human Milk: Results from a Prospective Study.
32041195	6	63	theme	fourth	846:851	arg1	quartile					853:860	the fourth quartile	842:860	the fourth quartile of volume	842:870	Protein content in the fourth quartile of volume was significantly lower than that in the first three, suggesting the existence of a volume threshold for protein content at approximately 445 mL.
32041195	0	64	dep	Role	0:3	arg1	Results					85:91	Results	85:91	Role of Daily Milk Volume and Period of Lactation in Nutrient Content of Human Milk: Results from a Prospective Study.	0:117	Role of Daily Milk Volume and Period of Lactation in Nutrient Content of Human Milk: Results from a Prospective Study.
32041195	0	65	from	Role	0:3	arg1	Content					62:68	Nutrient Content	53:68	Nutrient Content of Human Milk	53:82	Role of Daily Milk Volume and Period of Lactation in Nutrient Content of Human Milk: Results from a Prospective Study.
32041195	8	66	dep	along	1320:1324	arg1	with					1326:1329	with	1326:1329	with	1326:1329	In consideration of previous findings along with our data, we suggest that extra care should be taken with fortification for feeding preterm infants when the mother's milk volume is greater than 400-450 mL.
32041195	7	67	theme	average	1079:1085	arg1	volume					1087:1092	average volume	1079:1092	average volume	1079:1092	After multivariate analysis, it was found that maternal age, average volume, and lactation period remained significantly associated with protein content, maternal age remained significantly associated with fat content, and only average volume with sodium content.
35484870	4	0	theme	infusion	634:641	arg1	safety					617:622	safety	617:622	safety	617:622	Secondary objectives included exploration of the efficacy and safety of an UFH infusion through the evaluation of organ function and incidence of minor and major bleeding.
35484870	4	0	theme	infusion	634:641	arg1	efficacy					604:611	efficacy	604:611	efficacy	604:611	Secondary objectives included exploration of the efficacy and safety of an UFH infusion through the evaluation of organ function and incidence of minor and major bleeding.
35484870	8	1	theme	significant	1188:1198	arg1	increase					1200:1207	a significant increase	1186:1207	a significant increase in median D-dimer concentrations from day 1 to day 7 in the control group (475 ng/mL [291-999] vs. 10820 ng/mL [606-21033]	1186:1330	There was a significant increase in median D-dimer concentrations from day 1 to day 7 in the control group (475 ng/mL [291-999] vs. 10820 ng/mL [606-21033], P = 0.04).
35484870	3	2	with	patients	436:443	arg1	COVID-19					450:457	COVID-19	450:457	COVID-19	450:457	OBJECTIVES The primary objective was to compare the incidence of thromboembolic events in adult patients with COVID-19 treated with an unfractionated heparin (UFH) infusion versus prophylactic dose anticoagulation.
35484870	9	3	contain	had	1370:1372	arg1	Patients					1344:1351	Patients	1344:1351	Patients treated with UFH	1344:1368	Patients treated with UFH had a higher incidence of minor bleeding (35.7% vs. 0%, P < 0.005) and required more units of packed red blood cell transfusion (0.8 units ± 1.6 vs. 0 units, P = 0.01).
35484870	9	3	contain	had	1370:1372	arg2	incidence					1383:1391	a higher incidence	1374:1391	a higher incidence of minor bleeding (35.7% vs. 0%, P < 0.005)	1374:1435	Patients treated with UFH had a higher incidence of minor bleeding (35.7% vs. 0%, P < 0.005) and required more units of packed red blood cell transfusion (0.8 units ± 1.6 vs. 0 units, P = 0.01).
35484870	9	4	theme	bleeding	1402:1409	arg1	incidence					1383:1391	a higher incidence	1374:1391	a higher incidence of minor bleeding (35.7% vs. 0%, P < 0.005)	1374:1435	Patients treated with UFH had a higher incidence of minor bleeding (35.7% vs. 0%, P < 0.005) and required more units of packed red blood cell transfusion (0.8 units ± 1.6 vs. 0 units, P = 0.01).
35484870	1	5	theme	severe	191:196	arg1	hypercoagulability					198:215	severe hypercoagulability	191:215	severe hypercoagulability	191:215	INTRODUCTION The Coronavirus Disease 2019 (COVID-19) is associated with severe hypercoagulability.
35484870	8	6	theme	D-dimer	1219:1225	arg1	concentrations					1227:1240	median D-dimer concentrations	1212:1240	median D-dimer concentrations from day 1 to day 7	1212:1260	There was a significant increase in median D-dimer concentrations from day 1 to day 7 in the control group (475 ng/mL [291-999] vs. 10820 ng/mL [606-21033], P = 0.04).
35484870	8	7	from	day	1247:1249	arg1	concentrations					1227:1240	median D-dimer concentrations	1212:1240	median D-dimer concentrations from day 1 to day 7	1212:1260	There was a significant increase in median D-dimer concentrations from day 1 to day 7 in the control group (475 ng/mL [291-999] vs. 10820 ng/mL [606-21033], P = 0.04).
35484870	8	7	from	day	1247:1249	arg1	increase					1200:1207	a significant increase	1186:1207	a significant increase in median D-dimer concentrations from day 1 to day 7 in the control group (475 ng/mL [291-999] vs. 10820 ng/mL [606-21033]	1186:1330	There was a significant increase in median D-dimer concentrations from day 1 to day 7 in the control group (475 ng/mL [291-999] vs. 10820 ng/mL [606-21033], P = 0.04).
35484870	8	8	theme	control	1269:1275	arg1	group					1277:1281	the control group	1265:1281	the control group (475 ng/mL [291-999] vs. 10820 ng/mL [606-21033]	1265:1330	There was a significant increase in median D-dimer concentrations from day 1 to day 7 in the control group (475 ng/mL [291-999] vs. 10820 ng/mL [606-21033], P = 0.04).
35484870	2	9	theme	COVID-19	330:337	arg1	setting					319:325	the setting	315:325	the setting of COVID-19	315:337	There is currently limited evidence supporting the routine use of therapeutic anticoagulation in the setting of COVID-19.
35484870	4	10	theme	minor	701:705	arg1	exploration					585:595	exploration	585:595	exploration of the efficacy and safety of an UFH infusion through the evaluation of organ function	585:682	Secondary objectives included exploration of the efficacy and safety of an UFH infusion through the evaluation of organ function and incidence of minor and major bleeding.
35484870	4	10	theme	minor	701:705	arg1	incidence					688:696	incidence	688:696	incidence of minor and major bleeding	688:724	Secondary objectives included exploration of the efficacy and safety of an UFH infusion through the evaluation of organ function and incidence of minor and major bleeding.
35484870	11	11	theme	concentrations	1735:1748	arg1	stabilization					1710:1722	stabilization	1710:1722	stabilization of D-dimer concentrations	1710:1748	UFH was associated with stabilization of D-dimer concentrations and increased rates of minor bleeding and transfusions.
35484870	11	11	theme	concentrations	1735:1748	arg1	rates					1764:1768	increased rates	1754:1768	increased rates of minor bleeding and transfusions	1754:1803	UFH was associated with stabilization of D-dimer concentrations and increased rates of minor bleeding and transfusions.
35484870	3	12	from	incidence	392:400	arg1	patients					436:443	adult patients	430:443	adult patients with COVID-19 treated with an unfractionated heparin (UFH) infusion versus prophylactic dose anticoagulation	430:552	OBJECTIVES The primary objective was to compare the incidence of thromboembolic events in adult patients with COVID-19 treated with an unfractionated heparin (UFH) infusion versus prophylactic dose anticoagulation.
35484870	7	13	theme	catheter-related	1086:1101	arg1	thrombosis					1103:1112	catheter-related thrombosis	1086:1112	catheter-related thrombosis	1086:1112	There was no difference in the composite of thromboembolic events comprised of venous thromboembolism, arterial thrombosis, and catheter-related thrombosis between the UFH and control group (17.9% vs. 3.6%, P = 0.19).
35484870	5	14	theme	Retrospective	735:747	arg1	study					770:774	Retrospective observational cohort study	735:774	METHODS Retrospective observational cohort study with propensity score matching of COVID-19 patients who received an UFH infusion targeting an aPTT between 40 and 60 seconds.	727:900	METHODS Retrospective observational cohort study with propensity score matching of COVID-19 patients who received an UFH infusion targeting an aPTT between 40 and 60 seconds.
35484870	1	15	theme	Disease	148:154	arg1	2019					156:159	The Coronavirus Disease 2019	132:159	The Coronavirus Disease 2019 (COVID-19)	132:170	INTRODUCTION The Coronavirus Disease 2019 (COVID-19) is associated with severe hypercoagulability.
35484870	1	15	theme	Disease	148:154	arg1	COVID-19					162:169	COVID-19	162:169	COVID-19	162:169	INTRODUCTION The Coronavirus Disease 2019 (COVID-19) is associated with severe hypercoagulability.
35484870	8	16	theme	10820	1308:1312	arg1	ng/mL					1314:1318	10820 ng/mL [606-21033	1308:1329	10820 ng/mL [606-21033	1308:1329	There was a significant increase in median D-dimer concentrations from day 1 to day 7 in the control group (475 ng/mL [291-999] vs. 10820 ng/mL [606-21033], P = 0.04).
35484870	5	17	theme	cohort	763:768	arg1	study					770:774	Retrospective observational cohort study	735:774	METHODS Retrospective observational cohort study with propensity score matching of COVID-19 patients who received an UFH infusion targeting an aPTT between 40 and 60 seconds.	727:900	METHODS Retrospective observational cohort study with propensity score matching of COVID-19 patients who received an UFH infusion targeting an aPTT between 40 and 60 seconds.
35484870	2	18	theme	therapeutic	284:294	arg1	anticoagulation					296:310	therapeutic anticoagulation	284:310	therapeutic anticoagulation	284:310	There is currently limited evidence supporting the routine use of therapeutic anticoagulation in the setting of COVID-19.
35484870	7	19	theme	arterial	1061:1068	arg1	thrombosis					1070:1079	arterial thrombosis	1061:1079	arterial thrombosis	1061:1079	There was no difference in the composite of thromboembolic events comprised of venous thromboembolism, arterial thrombosis, and catheter-related thrombosis between the UFH and control group (17.9% vs. 3.6%, P = 0.19).
35484870	1	20	dep	INTRODUCTION	119:130	arg1	associated					175:184	associated	175:184	is associated with severe hypercoagulability	172:215	INTRODUCTION The Coronavirus Disease 2019 (COVID-19) is associated with severe hypercoagulability.
35484870	9	21	theme	more	1450:1453	arg1	units					1455:1459	more units	1450:1459	more units of packed red blood cell transfusion (0.8 units ± 1.6 vs. 0 units, P = 0.01)	1450:1536	Patients treated with UFH had a higher incidence of minor bleeding (35.7% vs. 0%, P < 0.005) and required more units of packed red blood cell transfusion (0.8 units ± 1.6 vs. 0 units, P = 0.01).
35484870	3	22	theme	primary	355:361	arg1	objective					363:371	The primary objective	351:371	The primary objective	351:371	OBJECTIVES The primary objective was to compare the incidence of thromboembolic events in adult patients with COVID-19 treated with an unfractionated heparin (UFH) infusion versus prophylactic dose anticoagulation.
35484870	9	23	dep	transfusion	1486:1496	arg1	=					1530:1530	=	1530:1530	=	1530:1530	Patients treated with UFH had a higher incidence of minor bleeding (35.7% vs. 0%, P < 0.005) and required more units of packed red blood cell transfusion (0.8 units ± 1.6 vs. 0 units, P = 0.01).
35484870	5	24	theme	UFH	844:846	arg1	infusion					848:855	an UFH infusion	841:855	an UFH infusion targeting an aPTT between 40 and 60 seconds	841:899	METHODS Retrospective observational cohort study with propensity score matching of COVID-19 patients who received an UFH infusion targeting an aPTT between 40 and 60 seconds.
35484870	7	25	theme	events	1017:1022	arg1	composite					989:997	the composite	985:997	the composite of thromboembolic events comprised of venous thromboembolism, arterial thrombosis, and catheter-related thrombosis	985:1112	There was no difference in the composite of thromboembolic events comprised of venous thromboembolism, arterial thrombosis, and catheter-related thrombosis between the UFH and control group (17.9% vs. 3.6%, P = 0.19).
35484870	7	25	theme	events	1017:1022	arg1	events					1017:1022	thromboembolic events	1002:1022	thromboembolic events comprised of venous thromboembolism, arterial thrombosis, and catheter-related thrombosis	1002:1112	There was no difference in the composite of thromboembolic events comprised of venous thromboembolism, arterial thrombosis, and catheter-related thrombosis between the UFH and control group (17.9% vs. 3.6%, P = 0.19).
35484870	9	26	theme	red	1471:1473	arg1	transfusion					1486:1496	packed red blood cell transfusion	1464:1496	packed red blood cell transfusion (0.8 units ± 1.6 vs. 0 units, P = 0.01)	1464:1536	Patients treated with UFH had a higher incidence of minor bleeding (35.7% vs. 0%, P < 0.005) and required more units of packed red blood cell transfusion (0.8 units ± 1.6 vs. 0 units, P = 0.01).
35484870	5	27	dep	METHODS	727:733	arg1	study					770:774	Retrospective observational cohort study	735:774	METHODS Retrospective observational cohort study with propensity score matching of COVID-19 patients who received an UFH infusion targeting an aPTT between 40 and 60 seconds.	727:900	METHODS Retrospective observational cohort study with propensity score matching of COVID-19 patients who received an UFH infusion targeting an aPTT between 40 and 60 seconds.
35484870	3	28	theme	unfractionated	475:488	arg1	UFH					499:501	UFH	499:501	UFH	499:501	OBJECTIVES The primary objective was to compare the incidence of thromboembolic events in adult patients with COVID-19 treated with an unfractionated heparin (UFH) infusion versus prophylactic dose anticoagulation.
35484870	3	28	theme	unfractionated	475:488	arg1	heparin					490:496	unfractionated heparin	475:496	an unfractionated heparin (UFH) infusion	472:511	OBJECTIVES The primary objective was to compare the incidence of thromboembolic events in adult patients with COVID-19 treated with an unfractionated heparin (UFH) infusion versus prophylactic dose anticoagulation.
35484870	5	29	theme	patients	819:826	arg1	score					792:796	propensity score matching	781:805	propensity score matching of COVID-19 patients who received an UFH infusion targeting an aPTT between 40 and 60 seconds	781:899	METHODS Retrospective observational cohort study with propensity score matching of COVID-19 patients who received an UFH infusion targeting an aPTT between 40 and 60 seconds.
35484870	9	30	theme	cell	1481:1484	arg1	transfusion					1486:1496	packed red blood cell transfusion	1464:1496	packed red blood cell transfusion (0.8 units ± 1.6 vs. 0 units, P = 0.01)	1464:1536	Patients treated with UFH had a higher incidence of minor bleeding (35.7% vs. 0%, P < 0.005) and required more units of packed red blood cell transfusion (0.8 units ± 1.6 vs. 0 units, P = 0.01).
35484870	5	31	theme	propensity	781:790	arg1	score					792:796	propensity score matching	781:805	propensity score matching of COVID-19 patients who received an UFH infusion targeting an aPTT between 40 and 60 seconds	781:899	METHODS Retrospective observational cohort study with propensity score matching of COVID-19 patients who received an UFH infusion targeting an aPTT between 40 and 60 seconds.
35484870	0	32	theme	Continuous	0:9	arg1	Heparin					44:50	Continuous Infusion Low-Dose Unfractionated Heparin	0:50	Continuous Infusion Low-Dose Unfractionated Heparin for the Management of Hypercoagulability	0:91	Continuous Infusion Low-Dose Unfractionated Heparin for the Management of Hypercoagulability Associated With COVID-19.
35484870	3	33	theme	thromboembolic	405:418	arg1	events					420:425	thromboembolic events	405:425	thromboembolic events	405:425	OBJECTIVES The primary objective was to compare the incidence of thromboembolic events in adult patients with COVID-19 treated with an unfractionated heparin (UFH) infusion versus prophylactic dose anticoagulation.
35484870	7	34	from	difference	971:980	arg1	composite					989:997	the composite	985:997	the composite of thromboembolic events comprised of venous thromboembolism, arterial thrombosis, and catheter-related thrombosis	985:1112	There was no difference in the composite of thromboembolic events comprised of venous thromboembolism, arterial thrombosis, and catheter-related thrombosis between the UFH and control group (17.9% vs. 3.6%, P = 0.19).
35484870	7	34	from	difference	971:980	arg1	events					1017:1022	thromboembolic events	1002:1022	thromboembolic events comprised of venous thromboembolism, arterial thrombosis, and catheter-related thrombosis	1002:1112	There was no difference in the composite of thromboembolic events comprised of venous thromboembolism, arterial thrombosis, and catheter-related thrombosis between the UFH and control group (17.9% vs. 3.6%, P = 0.19).
35484870	10	35	theme	UFH	1573:1575	arg1	infusion					1561:1568	CONCLUSION Continuous infusion	1539:1568	CONCLUSION Continuous infusion of UFH for patients with COVID-19 infection	1539:1612	CONCLUSION Continuous infusion of UFH for patients with COVID-19 infection did not decrease the overall incidence of thromboembolic complications.
35484870	0	36	theme	Low-Dose	20:27	arg1	Heparin					44:50	Continuous Infusion Low-Dose Unfractionated Heparin	0:50	Continuous Infusion Low-Dose Unfractionated Heparin for the Management of Hypercoagulability	0:91	Continuous Infusion Low-Dose Unfractionated Heparin for the Management of Hypercoagulability Associated With COVID-19.
35484870	10	37	with	patients	1581:1588	arg1	infection					1604:1612	COVID-19 infection	1595:1612	COVID-19 infection	1595:1612	CONCLUSION Continuous infusion of UFH for patients with COVID-19 infection did not decrease the overall incidence of thromboembolic complications.
35484870	9	38	theme	units	1503:1507	arg1	±					1509:1509	0.8 units ± 1.6 vs. 0 units	1499:1525	0.8 units ± 1.6 vs. 0 units	1499:1525	Patients treated with UFH had a higher incidence of minor bleeding (35.7% vs. 0%, P < 0.005) and required more units of packed red blood cell transfusion (0.8 units ± 1.6 vs. 0 units, P = 0.01).
35484870	6	39	dep	RESULTS	902:908	arg1	patients					920:927	Fifty-six patients	910:927	RESULTS Fifty-six patients	902:927	RESULTS Fifty-six patients were included in this study.
35484870	9	40	dep	%	1416:1416	arg1	0.005					1430:1434	0.005	1430:1434	0.005	1430:1434	Patients treated with UFH had a higher incidence of minor bleeding (35.7% vs. 0%, P < 0.005) and required more units of packed red blood cell transfusion (0.8 units ± 1.6 vs. 0 units, P = 0.01).
35484870	10	41	theme	COVID-19	1595:1602	arg1	infection					1604:1612	COVID-19 infection	1595:1612	COVID-19 infection	1595:1612	CONCLUSION Continuous infusion of UFH for patients with COVID-19 infection did not decrease the overall incidence of thromboembolic complications.
35484870	6	42	theme	Fifty-six	910:918	arg1	patients					920:927	Fifty-six patients	910:927	RESULTS Fifty-six patients	902:927	RESULTS Fifty-six patients were included in this study.
35484870	11	43	theme	increased	1754:1762	arg1	rates					1764:1768	increased rates	1754:1768	increased rates of minor bleeding and transfusions	1754:1803	UFH was associated with stabilization of D-dimer concentrations and increased rates of minor bleeding and transfusions.
35484870	11	44	theme	transfusions	1792:1803	arg1	stabilization					1710:1722	stabilization	1710:1722	stabilization of D-dimer concentrations	1710:1748	UFH was associated with stabilization of D-dimer concentrations and increased rates of minor bleeding and transfusions.
35484870	11	44	theme	transfusions	1792:1803	arg1	rates					1764:1768	increased rates	1754:1768	increased rates of minor bleeding and transfusions	1754:1803	UFH was associated with stabilization of D-dimer concentrations and increased rates of minor bleeding and transfusions.
35484870	4	45	theme	organ	669:673	arg1	function					675:682	organ function	669:682	organ function	669:682	Secondary objectives included exploration of the efficacy and safety of an UFH infusion through the evaluation of organ function and incidence of minor and major bleeding.
35484870	11	46	theme	bleeding	1779:1786	arg1	stabilization					1710:1722	stabilization	1710:1722	stabilization of D-dimer concentrations	1710:1748	UFH was associated with stabilization of D-dimer concentrations and increased rates of minor bleeding and transfusions.
35484870	11	46	theme	bleeding	1779:1786	arg1	rates					1764:1768	increased rates	1754:1768	increased rates of minor bleeding and transfusions	1754:1803	UFH was associated with stabilization of D-dimer concentrations and increased rates of minor bleeding and transfusions.
35484870	4	47	theme	UFH	630:632	arg1	infusion					634:641	an UFH infusion	627:641	an UFH infusion through the evaluation of organ function	627:682	Secondary objectives included exploration of the efficacy and safety of an UFH infusion through the evaluation of organ function and incidence of minor and major bleeding.
35484870	3	48	dep	OBJECTIVES	340:349	arg1	was					373:375	was	373:375	was to compare the incidence of thromboembolic events in adult patients with COVID-19 treated with an unfractionated heparin (UFH) infusion versus prophylactic dose anticoagulation	373:552	OBJECTIVES The primary objective was to compare the incidence of thromboembolic events in adult patients with COVID-19 treated with an unfractionated heparin (UFH) infusion versus prophylactic dose anticoagulation.
35484870	3	49	theme	dose	533:536	arg1	anticoagulation					538:552	prophylactic dose anticoagulation	520:552	prophylactic dose anticoagulation	520:552	OBJECTIVES The primary objective was to compare the incidence of thromboembolic events in adult patients with COVID-19 treated with an unfractionated heparin (UFH) infusion versus prophylactic dose anticoagulation.
35484870	10	50	theme	overall	1635:1641	arg1	incidence					1643:1651	the overall incidence	1631:1651	the overall incidence of thromboembolic complications	1631:1683	CONCLUSION Continuous infusion of UFH for patients with COVID-19 infection did not decrease the overall incidence of thromboembolic complications.
35484870	9	51	dep	±	1509:1509	arg1	units					1521:1525	1.6 vs. 0 units	1511:1525	0.8 units ± 1.6 vs. 0 units	1499:1525	Patients treated with UFH had a higher incidence of minor bleeding (35.7% vs. 0%, P < 0.005) and required more units of packed red blood cell transfusion (0.8 units ± 1.6 vs. 0 units, P = 0.01).
35484870	8	52	theme	median	1212:1217	arg1	concentrations					1227:1240	median D-dimer concentrations	1212:1240	median D-dimer concentrations from day 1 to day 7	1212:1260	There was a significant increase in median D-dimer concentrations from day 1 to day 7 in the control group (475 ng/mL [291-999] vs. 10820 ng/mL [606-21033], P = 0.04).
35484870	4	53	theme	bleeding	717:724	arg1	exploration					585:595	exploration	585:595	exploration of the efficacy and safety of an UFH infusion through the evaluation of organ function	585:682	Secondary objectives included exploration of the efficacy and safety of an UFH infusion through the evaluation of organ function and incidence of minor and major bleeding.
35484870	4	53	theme	bleeding	717:724	arg1	incidence					688:696	incidence	688:696	incidence of minor and major bleeding	688:724	Secondary objectives included exploration of the efficacy and safety of an UFH infusion through the evaluation of organ function and incidence of minor and major bleeding.
35484870	7	54	dep	=	1167:1167	arg1	%					1153:1153	17.9%	1149:1153	17.9%	1149:1153	There was no difference in the composite of thromboembolic events comprised of venous thromboembolism, arterial thrombosis, and catheter-related thrombosis between the UFH and control group (17.9% vs. 3.6%, P = 0.19).
35484870	7	54	dep	=	1167:1167	arg1	%					1162:1162	3.6%	1159:1162	3.6%	1159:1162	There was no difference in the composite of thromboembolic events comprised of venous thromboembolism, arterial thrombosis, and catheter-related thrombosis between the UFH and control group (17.9% vs. 3.6%, P = 0.19).
35484870	10	55	theme	complications	1671:1683	arg1	incidence					1643:1651	the overall incidence	1631:1651	the overall incidence of thromboembolic complications	1631:1683	CONCLUSION Continuous infusion of UFH for patients with COVID-19 infection did not decrease the overall incidence of thromboembolic complications.
35484870	9	56	dep	=	1530:1530	arg1	±					1509:1509	0.8 units ± 1.6 vs. 0 units	1499:1525	0.8 units ± 1.6 vs. 0 units	1499:1525	Patients treated with UFH had a higher incidence of minor bleeding (35.7% vs. 0%, P < 0.005) and required more units of packed red blood cell transfusion (0.8 units ± 1.6 vs. 0 units, P = 0.01).
35484870	7	57	theme	control	1134:1140	arg1	group					1142:1146	the UFH and control group	1122:1146	group	1142:1146	There was no difference in the composite of thromboembolic events comprised of venous thromboembolism, arterial thrombosis, and catheter-related thrombosis between the UFH and control group (17.9% vs. 3.6%, P = 0.19).
35484870	2	58	theme	routine	269:275	arg1	use					277:279	the routine use	265:279	the routine use of therapeutic anticoagulation in the setting of COVID-19	265:337	There is currently limited evidence supporting the routine use of therapeutic anticoagulation in the setting of COVID-19.
35484870	7	59	theme	UFH	1126:1128	arg1	group					1142:1146	the UFH and control group	1122:1146	group	1142:1146	There was no difference in the composite of thromboembolic events comprised of venous thromboembolism, arterial thrombosis, and catheter-related thrombosis between the UFH and control group (17.9% vs. 3.6%, P = 0.19).
35484870	1	60	theme	Coronavirus	136:146	arg1	2019					156:159	The Coronavirus Disease 2019	132:159	The Coronavirus Disease 2019 (COVID-19)	132:170	INTRODUCTION The Coronavirus Disease 2019 (COVID-19) is associated with severe hypercoagulability.
35484870	1	60	theme	Coronavirus	136:146	arg1	COVID-19					162:169	COVID-19	162:169	COVID-19	162:169	INTRODUCTION The Coronavirus Disease 2019 (COVID-19) is associated with severe hypercoagulability.
35484870	11	61	theme	D-dimer	1727:1733	arg1	concentrations					1735:1748	D-dimer concentrations	1727:1748	D-dimer concentrations	1727:1748	UFH was associated with stabilization of D-dimer concentrations and increased rates of minor bleeding and transfusions.
35484870	5	62	theme	observational	749:761	arg1	study					770:774	Retrospective observational cohort study	735:774	METHODS Retrospective observational cohort study with propensity score matching of COVID-19 patients who received an UFH infusion targeting an aPTT between 40 and 60 seconds.	727:900	METHODS Retrospective observational cohort study with propensity score matching of COVID-19 patients who received an UFH infusion targeting an aPTT between 40 and 60 seconds.
35484870	2	63	theme	anticoagulation	296:310	arg1	use					277:279	the routine use	265:279	the routine use of therapeutic anticoagulation in the setting of COVID-19	265:337	There is currently limited evidence supporting the routine use of therapeutic anticoagulation in the setting of COVID-19.
35484870	0	64	theme	Hypercoagulability	74:91	arg1	Management					60:69	the Management	56:69	the Management of Hypercoagulability	56:91	Continuous Infusion Low-Dose Unfractionated Heparin for the Management of Hypercoagulability Associated With COVID-19.
35484870	4	65	theme	Secondary	555:563	arg1	objectives					565:574	Secondary objectives	555:574	Secondary objectives	555:574	Secondary objectives included exploration of the efficacy and safety of an UFH infusion through the evaluation of organ function and incidence of minor and major bleeding.
35484870	5	66	theme	matching	798:805	arg1	score					792:796	propensity score matching	781:805	propensity score matching of COVID-19 patients who received an UFH infusion targeting an aPTT between 40 and 60 seconds	781:899	METHODS Retrospective observational cohort study with propensity score matching of COVID-19 patients who received an UFH infusion targeting an aPTT between 40 and 60 seconds.
35484870	7	67	theme	venous	1037:1042	arg1	thromboembolism					1044:1058	venous thromboembolism	1037:1058	venous thromboembolism	1037:1058	There was no difference in the composite of thromboembolic events comprised of venous thromboembolism, arterial thrombosis, and catheter-related thrombosis between the UFH and control group (17.9% vs. 3.6%, P = 0.19).
35484870	9	68	theme	packed	1464:1469	arg1	transfusion					1486:1496	packed red blood cell transfusion	1464:1496	packed red blood cell transfusion (0.8 units ± 1.6 vs. 0 units, P = 0.01)	1464:1536	Patients treated with UFH had a higher incidence of minor bleeding (35.7% vs. 0%, P < 0.005) and required more units of packed red blood cell transfusion (0.8 units ± 1.6 vs. 0 units, P = 0.01).
35484870	10	69	theme	thromboembolic	1656:1669	arg1	complications					1671:1683	thromboembolic complications	1656:1683	thromboembolic complications	1656:1683	CONCLUSION Continuous infusion of UFH for patients with COVID-19 infection did not decrease the overall incidence of thromboembolic complications.
35484870	9	70	theme	blood	1475:1479	arg1	transfusion					1486:1496	packed red blood cell transfusion	1464:1496	packed red blood cell transfusion (0.8 units ± 1.6 vs. 0 units, P = 0.01)	1464:1536	Patients treated with UFH had a higher incidence of minor bleeding (35.7% vs. 0%, P < 0.005) and required more units of packed red blood cell transfusion (0.8 units ± 1.6 vs. 0 units, P = 0.01).
35484870	3	71	theme	heparin	490:496	arg1	infusion					504:511	an unfractionated heparin (UFH) infusion	472:511	an unfractionated heparin (UFH) infusion	472:511	OBJECTIVES The primary objective was to compare the incidence of thromboembolic events in adult patients with COVID-19 treated with an unfractionated heparin (UFH) infusion versus prophylactic dose anticoagulation.
35484870	0	72	theme	Infusion	11:18	arg1	Heparin					44:50	Continuous Infusion Low-Dose Unfractionated Heparin	0:50	Continuous Infusion Low-Dose Unfractionated Heparin for the Management of Hypercoagulability	0:91	Continuous Infusion Low-Dose Unfractionated Heparin for the Management of Hypercoagulability Associated With COVID-19.
35484870	7	73	theme	thromboembolic	1002:1015	arg1	events					1017:1022	thromboembolic events	1002:1022	thromboembolic events comprised of venous thromboembolism, arterial thrombosis, and catheter-related thrombosis	1002:1112	There was no difference in the composite of thromboembolic events comprised of venous thromboembolism, arterial thrombosis, and catheter-related thrombosis between the UFH and control group (17.9% vs. 3.6%, P = 0.19).
35484870	9	74	theme	transfusion	1486:1496	arg1	units					1455:1459	more units	1450:1459	more units of packed red blood cell transfusion (0.8 units ± 1.6 vs. 0 units, P = 0.01)	1450:1536	Patients treated with UFH had a higher incidence of minor bleeding (35.7% vs. 0%, P < 0.005) and required more units of packed red blood cell transfusion (0.8 units ± 1.6 vs. 0 units, P = 0.01).
35484870	3	75	theme	adult	430:434	arg1	patients					436:443	adult patients	430:443	adult patients with COVID-19 treated with an unfractionated heparin (UFH) infusion versus prophylactic dose anticoagulation	430:552	OBJECTIVES The primary objective was to compare the incidence of thromboembolic events in adult patients with COVID-19 treated with an unfractionated heparin (UFH) infusion versus prophylactic dose anticoagulation.
35484870	4	76	theme	safety	617:622	arg1	exploration					585:595	exploration	585:595	exploration of the efficacy and safety of an UFH infusion through the evaluation of organ function	585:682	Secondary objectives included exploration of the efficacy and safety of an UFH infusion through the evaluation of organ function and incidence of minor and major bleeding.
35484870	4	76	theme	safety	617:622	arg1	incidence					688:696	incidence	688:696	incidence of minor and major bleeding	688:724	Secondary objectives included exploration of the efficacy and safety of an UFH infusion through the evaluation of organ function and incidence of minor and major bleeding.
35484870	0	77	theme	Unfractionated	29:42	arg1	Heparin					44:50	Continuous Infusion Low-Dose Unfractionated Heparin	0:50	Continuous Infusion Low-Dose Unfractionated Heparin for the Management of Hypercoagulability	0:91	Continuous Infusion Low-Dose Unfractionated Heparin for the Management of Hypercoagulability Associated With COVID-19.
35484870	2	78	from	use	277:279	arg1	setting					319:325	the setting	315:325	the setting of COVID-19	315:337	There is currently limited evidence supporting the routine use of therapeutic anticoagulation in the setting of COVID-19.
35484870	3	79	theme	events	420:425	arg1	incidence					392:400	the incidence	388:400	the incidence of thromboembolic events in adult patients with COVID-19 treated with an unfractionated heparin (UFH) infusion versus prophylactic dose anticoagulation	388:552	OBJECTIVES The primary objective was to compare the incidence of thromboembolic events in adult patients with COVID-19 treated with an unfractionated heparin (UFH) infusion versus prophylactic dose anticoagulation.
35484870	5	80	with	study	770:774	arg1	score					792:796	propensity score matching	781:805	propensity score matching of COVID-19 patients who received an UFH infusion targeting an aPTT between 40 and 60 seconds	781:899	METHODS Retrospective observational cohort study with propensity score matching of COVID-19 patients who received an UFH infusion targeting an aPTT between 40 and 60 seconds.
35484870	10	81	theme	CONCLUSION	1539:1548	arg1	infusion					1561:1568	CONCLUSION Continuous infusion	1539:1568	CONCLUSION Continuous infusion of UFH for patients with COVID-19 infection	1539:1612	CONCLUSION Continuous infusion of UFH for patients with COVID-19 infection did not decrease the overall incidence of thromboembolic complications.
35484870	10	82	theme	Continuous	1550:1559	arg1	infusion					1561:1568	CONCLUSION Continuous infusion	1539:1568	CONCLUSION Continuous infusion of UFH for patients with COVID-19 infection	1539:1612	CONCLUSION Continuous infusion of UFH for patients with COVID-19 infection did not decrease the overall incidence of thromboembolic complications.
35484870	4	83	theme	efficacy	604:611	arg1	exploration					585:595	exploration	585:595	exploration of the efficacy and safety of an UFH infusion through the evaluation of organ function	585:682	Secondary objectives included exploration of the efficacy and safety of an UFH infusion through the evaluation of organ function and incidence of minor and major bleeding.
35484870	4	83	theme	efficacy	604:611	arg1	incidence					688:696	incidence	688:696	incidence of minor and major bleeding	688:724	Secondary objectives included exploration of the efficacy and safety of an UFH infusion through the evaluation of organ function and incidence of minor and major bleeding.
35484870	8	84	from	increase	1200:1207	arg1	concentrations					1227:1240	median D-dimer concentrations	1212:1240	median D-dimer concentrations from day 1 to day 7	1212:1260	There was a significant increase in median D-dimer concentrations from day 1 to day 7 in the control group (475 ng/mL [291-999] vs. 10820 ng/mL [606-21033], P = 0.04).
35484870	8	84	from	increase	1200:1207	arg1	day					1247:1249	day 1	1247:1251	day 1 to day 7	1247:1260	There was a significant increase in median D-dimer concentrations from day 1 to day 7 in the control group (475 ng/mL [291-999] vs. 10820 ng/mL [606-21033], P = 0.04).
35484870	8	84	from	increase	1200:1207	arg1	group					1277:1281	the control group	1265:1281	the control group (475 ng/mL [291-999] vs. 10820 ng/mL [606-21033]	1265:1330	There was a significant increase in median D-dimer concentrations from day 1 to day 7 in the control group (475 ng/mL [291-999] vs. 10820 ng/mL [606-21033], P = 0.04).
35484870	8	85	dep	group	1277:1281	arg1	ng/mL					1314:1318	10820 ng/mL [606-21033	1308:1329	10820 ng/mL [606-21033	1308:1329	There was a significant increase in median D-dimer concentrations from day 1 to day 7 in the control group (475 ng/mL [291-999] vs. 10820 ng/mL [606-21033], P = 0.04).
35484870	8	85	dep	group	1277:1281	arg1	ng/mL					1288:1292	475 ng/mL [291-999]	1284:1302	475 ng/mL [291-999]	1284:1302	There was a significant increase in median D-dimer concentrations from day 1 to day 7 in the control group (475 ng/mL [291-999] vs. 10820 ng/mL [606-21033], P = 0.04).
35484870	3	86	theme	prophylactic	520:531	arg1	anticoagulation					538:552	prophylactic dose anticoagulation	520:552	prophylactic dose anticoagulation	520:552	OBJECTIVES The primary objective was to compare the incidence of thromboembolic events in adult patients with COVID-19 treated with an unfractionated heparin (UFH) infusion versus prophylactic dose anticoagulation.
35484870	4	87	theme	function	675:682	arg1	evaluation					655:664	the evaluation	651:664	the evaluation of organ function	651:682	Secondary objectives included exploration of the efficacy and safety of an UFH infusion through the evaluation of organ function and incidence of minor and major bleeding.
35484870	9	88	theme	higher	1376:1381	arg1	incidence					1383:1391	a higher incidence	1374:1391	a higher incidence of minor bleeding (35.7% vs. 0%, P < 0.005)	1374:1435	Patients treated with UFH had a higher incidence of minor bleeding (35.7% vs. 0%, P < 0.005) and required more units of packed red blood cell transfusion (0.8 units ± 1.6 vs. 0 units, P = 0.01).
34333003	0	0	theme	nanoparticles	109:121	arg1	hybrids					69:75	hybrids	69:75	hybrids of nanofibrillated chitosan/ZnO nanoparticles	69:121	Design and characterization of keratin/PVA-PLA nanofibers containing hybrids of nanofibrillated chitosan/ZnO nanoparticles.
34333003	5	1	theme	roughness	977:985	arg1	factor					987:992	the surface roughness factor	965:992	the surface roughness factor for these nanofibers	965:1013	AFM results for K/PVA-PLA, K/PVA/CS-PLA/CS and K/PVA/CSZ(2:1)-PLA/CSZ(2:1), K/PVA/CSZ(1:2)-PLA/CSZ(1:2) samples indicated that the surface roughness factor for these nanofibers was about 708, 277, 378 and 658 nm, respectively.
34333003	6	2	theme	1:2	1105:1107	arg1	structure					1110:1118	K/PVA/CSZ(1:2)-PLA/CSZ(1:2) structure	1082:1118	K/PVA/CSZ(1:2)-PLA/CSZ(1:2) structure	1082:1118	DSC analysis for K/PVA/CSZ(1:2)-PLA/CSZ(1:2) structure exhibited that the peaks related to the melting points of PLA and PVA shifted to higher temperatures.
34333003	1	3	theme	poly	193:196	arg1	ZnONPs					346:351	ZnONPs	346:351	ZnONPs	346:351	In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated.
34333003	1	3	theme	poly	193:196	arg1	CSZ					355:357	CSZ	355:357	CSZ	355:357	In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated.
34333003	1	3	theme	poly	193:196	arg1	nanoparticles					331:343	poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles	193:343	poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination	193:370	In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated.
34333003	3	4	theme	nanofibers	718:727	arg1	diameter					706:713	the diameter	702:713	the diameter of nanofibers	702:727	Addition of CHNF and ZnONPs amounts in CSZ combination resulted in reduction of the diameter of nanofibers.
34333003	0	5	theme	chitosan/ZnO	96:107	arg1	nanoparticles					109:121	nanofibrillated chitosan/ZnO nanoparticles	80:121	nanofibrillated chitosan/ZnO nanoparticles	80:121	Design and characterization of keratin/PVA-PLA nanofibers containing hybrids of nanofibrillated chitosan/ZnO nanoparticles.
34333003	1	6	theme	/ZnO	326:329	arg1	ZnONPs					346:351	ZnONPs	346:351	ZnONPs	346:351	In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated.
34333003	1	6	theme	/ZnO	326:329	arg1	CSZ					355:357	CSZ	355:357	CSZ	355:357	In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated.
34333003	1	6	theme	/ZnO	326:329	arg1	nanoparticles					331:343	poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles	193:343	poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination	193:370	In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated.
34333003	7	7	with	nanofiber	1260:1268	arg1	diameter					1275:1282	diameter	1275:1282	diameter	1275:1282	Overally, K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa is suggested as a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation.
34333003	1	8	theme	nanoparticles	331:343	arg1	combination					360:370	poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination	193:370	poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination	193:370	In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated.
34333003	6	9	theme	-PLA/CSZ	1096:1103	arg1	structure					1110:1118	K/PVA/CSZ(1:2)-PLA/CSZ(1:2) structure	1082:1118	K/PVA/CSZ(1:2)-PLA/CSZ(1:2) structure	1082:1118	DSC analysis for K/PVA/CSZ(1:2)-PLA/CSZ(1:2) structure exhibited that the peaks related to the melting points of PLA and PVA shifted to higher temperatures.
34333003	7	10	theme	K/PVA/CSZ	1232:1240	arg1	nanofiber					1260:1268	K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber	1232:1268	K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa	1232:1363	Overally, K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa is suggested as a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation.
34333003	7	10	theme	K/PVA/CSZ	1232:1240	arg1	scaffold					1404:1411	a proper wound healing scaffold	1381:1411	a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation	1381:1494	Overally, K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa is suggested as a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation.
34333003	3	11	theme	diameter	706:713	arg1	reduction					689:697	reduction	689:697	reduction of the diameter of nanofibers	689:727	Addition of CHNF and ZnONPs amounts in CSZ combination resulted in reduction of the diameter of nanofibers.
34333003	7	12	theme	-PLA/CSZ	1246:1253	arg1	nanofiber					1260:1268	K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber	1232:1268	K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa	1232:1363	Overally, K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa is suggested as a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation.
34333003	7	12	theme	-PLA/CSZ	1246:1253	arg1	scaffold					1404:1411	a proper wound healing scaffold	1381:1411	a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation	1381:1494	Overally, K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa is suggested as a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation.
34333003	4	13	theme	K/PVA/CS-PLA/CS	774:788	arg1	sample					790:795	K/PVA/CS-PLA/CS sample	774:795	K/PVA/CS-PLA/CS sample with the contact angle of about 43 ± 3°	774:835	The highest hydrophilicity was reported for K/PVA/CS-PLA/CS sample with the contact angle of about 43 ± 3°.
34333003	7	14	theme	proper	1383:1388	arg1	healing					1396:1402	proper wound healing	1383:1402	a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation	1381:1494	Overally, K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa is suggested as a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation.
34333003	5	15	dep	K/PVA/CS-PLA/CS	865:879	arg1	samples					942:948	-PLA/CSZ(1:2) samples	928:948	-PLA/CSZ(1:2) samples	928:948	AFM results for K/PVA-PLA, K/PVA/CS-PLA/CS and K/PVA/CSZ(2:1)-PLA/CSZ(2:1), K/PVA/CSZ(1:2)-PLA/CSZ(1:2) samples indicated that the surface roughness factor for these nanofibers was about 708, 277, 378 and 658 nm, respectively.
34333003	6	16	theme	melting	1160:1166	arg1	points					1168:1173	the melting points	1156:1173	the melting points of PLA and PVA	1156:1188	DSC analysis for K/PVA/CSZ(1:2)-PLA/CSZ(1:2) structure exhibited that the peaks related to the melting points of PLA and PVA shifted to higher temperatures.
34333003	4	17	theme	highest	734:740	arg1	hydrophilicity					742:755	The highest hydrophilicity	730:755	The highest hydrophilicity	730:755	The highest hydrophilicity was reported for K/PVA/CS-PLA/CS sample with the contact angle of about 43 ± 3°.
34333003	7	18	theme	cell	1477:1480	arg1	proliferation					1482:1494	cell proliferation	1477:1494	cell proliferation	1477:1494	Overally, K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa is suggested as a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation.
34333003	1	19	theme	lactic	199:204	arg1	poly					193:196	poly	193:196	poly (lactic acid) (PLA)	193:216	In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated.
34333003	1	19	theme	lactic	199:204	arg1	acid					206:209	lactic acid	199:209	lactic acid	199:209	In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated.
34333003	7	20	theme	tensile	1329:1335	arg1	strength					1337:1344	tensile strength	1329:1344	tensile strength of 0.96 ± 0.18 MPa	1329:1363	Overally, K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa is suggested as a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation.
34333003	7	20	theme	tensile	1329:1335	arg1	352.50 ± 31 nm					1287:1300	352.50 ± 31 nm	1287:1300	352.50 ± 31 nm	1287:1300	Overally, K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa is suggested as a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation.
34333003	1	21	contain	containing	182:191	arg1	ingredient					390:399	the nanofiller ingredient	375:399	the nanofiller ingredient	375:399	In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated.
34333003	1	21	contain	containing	182:191	arg1	nanofibers					171:180	electrospun composite nanofibers	149:180	electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination	149:370	In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated.
34333003	1	21	contain	containing	182:191	arg2	combination					360:370	poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination	193:370	poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination	193:370	In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated.
34333003	5	22	theme	surface	969:975	arg1	factor					987:992	the surface roughness factor	965:992	the surface roughness factor for these nanofibers	965:1013	AFM results for K/PVA-PLA, K/PVA/CS-PLA/CS and K/PVA/CSZ(2:1)-PLA/CSZ(2:1), K/PVA/CSZ(1:2)-PLA/CSZ(1:2) samples indicated that the surface roughness factor for these nanofibers was about 708, 277, 378 and 658 nm, respectively.
34333003	6	23	theme	PLA	1178:1180	arg1	points					1168:1173	the melting points	1156:1173	the melting points of PLA and PVA	1156:1188	DSC analysis for K/PVA/CSZ(1:2)-PLA/CSZ(1:2) structure exhibited that the peaks related to the melting points of PLA and PVA shifted to higher temperatures.
34333003	6	24	theme	K/PVA/CSZ	1082:1090	arg1	structure					1110:1118	K/PVA/CSZ(1:2)-PLA/CSZ(1:2) structure	1082:1118	K/PVA/CSZ(1:2)-PLA/CSZ(1:2) structure	1082:1118	DSC analysis for K/PVA/CSZ(1:2)-PLA/CSZ(1:2) structure exhibited that the peaks related to the melting points of PLA and PVA shifted to higher temperatures.
34333003	5	25	theme	AFM	838:840	arg1	results					842:848	AFM results	838:848	AFM results for K/PVA-PLA, K/PVA/CS-PLA/CS and K/PVA/CSZ(2:1)-PLA/CSZ(2:1), K/PVA/CSZ(1:2)-PLA/CSZ(1:2) samples	838:948	AFM results for K/PVA-PLA, K/PVA/CS-PLA/CS and K/PVA/CSZ(2:1)-PLA/CSZ(2:1), K/PVA/CSZ(1:2)-PLA/CSZ(1:2) samples indicated that the surface roughness factor for these nanofibers was about 708, 277, 378 and 658 nm, respectively.
34333003	7	26	theme	352.50 ± 31 nm	1287:1300	arg1	nanofiber					1260:1268	K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber	1232:1268	K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa	1232:1363	Overally, K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa is suggested as a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation.
34333003	7	26	theme	352.50 ± 31 nm	1287:1300	arg1	scaffold					1404:1411	a proper wound healing scaffold	1381:1411	a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation	1381:1494	Overally, K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa is suggested as a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation.
34333003	7	27	theme	2:1	1255:1257	arg1	nanofiber					1260:1268	K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber	1232:1268	K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa	1232:1363	Overally, K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa is suggested as a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation.
34333003	7	27	theme	2:1	1255:1257	arg1	scaffold					1404:1411	a proper wound healing scaffold	1381:1411	a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation	1381:1494	Overally, K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa is suggested as a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation.
34333003	3	28	theme	ZnONPs	643:648	arg1	Addition					622:629	Addition	622:629	Addition of CHNF and ZnONPs amounts in CSZ combination	622:675	Addition of CHNF and ZnONPs amounts in CSZ combination resulted in reduction of the diameter of nanofibers.
34333003	2	29	theme	produced	563:570	arg1	nanofibers					572:581	produced nanofibers	563:581	produced nanofibers	563:581	PLA solution from one syringe and K/PVA from another one with incorporation of CHNF (CS), CSZ (2:1), (1:1) and (1:2) were electrospun and produced nanofibers were formed on the rotating collector.
34333003	6	30	theme	1:2	1092:1094	arg1	structure					1110:1118	K/PVA/CSZ(1:2)-PLA/CSZ(1:2) structure	1082:1118	K/PVA/CSZ(1:2)-PLA/CSZ(1:2) structure	1082:1118	DSC analysis for K/PVA/CSZ(1:2)-PLA/CSZ(1:2) structure exhibited that the peaks related to the melting points of PLA and PVA shifted to higher temperatures.
34333003	7	31	theme	48 ± 3°	1320:1326	arg1	352.50 ± 31 nm					1287:1300	352.50 ± 31 nm	1287:1300	352.50 ± 31 nm	1287:1300	Overally, K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa is suggested as a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation.
34333003	7	31	theme	48 ± 3°	1320:1326	arg1	angle					1311:1315	contact angle	1303:1315	contact angle of 48 ± 3°	1303:1326	Overally, K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa is suggested as a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation.
34333003	3	32	from	Addition	622:629	arg1	combination					665:675	CSZ combination	661:675	CSZ combination	661:675	Addition of CHNF and ZnONPs amounts in CSZ combination resulted in reduction of the diameter of nanofibers.
34333003	1	33	theme	major	266:270	arg1	components					272:281	the major components	262:281	the major components	262:281	In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated.
34333003	3	34	theme	CHNF	634:637	arg1	Addition					622:629	Addition	622:629	Addition of CHNF and ZnONPs amounts in CSZ combination	622:675	Addition of CHNF and ZnONPs amounts in CSZ combination resulted in reduction of the diameter of nanofibers.
34333003	6	35	theme	related	1145:1151	arg1	peaks					1139:1143	the peaks	1135:1143	the peaks related to the melting points of PLA and PVA	1135:1188	DSC analysis for K/PVA/CSZ(1:2)-PLA/CSZ(1:2) structure exhibited that the peaks related to the melting points of PLA and PVA shifted to higher temperatures.
34333003	5	36	theme	-PLA/CSZ	928:935	arg1	samples					942:948	-PLA/CSZ(1:2) samples	928:948	-PLA/CSZ(1:2) samples	928:948	AFM results for K/PVA-PLA, K/PVA/CS-PLA/CS and K/PVA/CSZ(2:1)-PLA/CSZ(2:1), K/PVA/CSZ(1:2)-PLA/CSZ(1:2) samples indicated that the surface roughness factor for these nanofibers was about 708, 277, 378 and 658 nm, respectively.
34333003	7	37	contain	has	1418:1420	arg2	antibacterial					1430:1442	antibacterial	1430:1442	antibacterial	1430:1442	Overally, K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa is suggested as a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation.
34333003	7	37	contain	has	1418:1420	arg1	nanofiber					1260:1268	K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber	1232:1268	K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa	1232:1363	Overally, K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa is suggested as a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation.
34333003	7	37	contain	has	1418:1420	arg2	potential					1455:1463	potential	1455:1463	highest antibacterial as well as potential	1422:1463	Overally, K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa is suggested as a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation.
34333003	7	37	contain	has	1418:1420	arg1	scaffold					1404:1411	a proper wound healing scaffold	1381:1411	a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation	1381:1494	Overally, K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa is suggested as a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation.
34333003	0	38	theme	nanofibers	47:56	arg1	characterization					11:26	characterization	11:26	characterization	11:26	Design and characterization of keratin/PVA-PLA nanofibers containing hybrids of nanofibrillated chitosan/ZnO nanoparticles.
34333003	0	38	theme	nanofibers	47:56	arg1	Design					0:5	Design	0:5	Design	0:5	Design and characterization of keratin/PVA-PLA nanofibers containing hybrids of nanofibrillated chitosan/ZnO nanoparticles.
34333003	2	39	theme	CHNF	504:507	arg1	incorporation					487:499	incorporation	487:499	incorporation of CHNF (CS)	487:512	PLA solution from one syringe and K/PVA from another one with incorporation of CHNF (CS), CSZ (2:1), (1:1) and (1:2) were electrospun and produced nanofibers were formed on the rotating collector.
34333003	2	40	from	syringe	447:453	arg1	solution					429:436	PLA solution	425:436	PLA solution from one syringe and K/PVA from another one with incorporation of CHNF (CS), CSZ (2:1), (1:1) and (1:2)	425:540	PLA solution from one syringe and K/PVA from another one with incorporation of CHNF (CS), CSZ (2:1), (1:1) and (1:2) were electrospun and produced nanofibers were formed on the rotating collector.
34333003	0	41	theme	keratin/PVA-PLA	31:45	arg1	nanofibers					47:56	keratin/PVA-PLA nanofibers	31:56	keratin/PVA-PLA nanofibers	31:56	Design and characterization of keratin/PVA-PLA nanofibers containing hybrids of nanofibrillated chitosan/ZnO nanoparticles.
34333003	1	42	theme	natural	287:293	arg1	/ZnO					326:329	natural nanofibrillated chitosan (CHNF)/ZnO	287:329	natural nanofibrillated chitosan (CHNF)/ZnO	287:329	In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated.
34333003	4	43	theme	43 ± 3°	829:835	arg1	angle					814:818	the contact angle	802:818	the contact angle of about 43 ± 3°	802:835	The highest hydrophilicity was reported for K/PVA/CS-PLA/CS sample with the contact angle of about 43 ± 3°.
34333003	5	44	theme	1:2	937:939	arg1	samples					942:948	-PLA/CSZ(1:2) samples	928:948	-PLA/CSZ(1:2) samples	928:948	AFM results for K/PVA-PLA, K/PVA/CS-PLA/CS and K/PVA/CSZ(2:1)-PLA/CSZ(2:1), K/PVA/CSZ(1:2)-PLA/CSZ(1:2) samples indicated that the surface roughness factor for these nanofibers was about 708, 277, 378 and 658 nm, respectively.
34333003	6	45	theme	DSC	1065:1067	arg1	analysis					1069:1076	DSC analysis	1065:1076	DSC analysis for K/PVA/CSZ(1:2)-PLA/CSZ(1:2) structure	1065:1118	DSC analysis for K/PVA/CSZ(1:2)-PLA/CSZ(1:2) structure exhibited that the peaks related to the melting points of PLA and PVA shifted to higher temperatures.
34333003	2	46	theme	rotating	602:609	arg1	collector					611:619	the rotating collector	598:619	the rotating collector	598:619	PLA solution from one syringe and K/PVA from another one with incorporation of CHNF (CS), CSZ (2:1), (1:1) and (1:2) were electrospun and produced nanofibers were formed on the rotating collector.
34333003	1	47	theme	keratin/poly	222:233	arg1	ZnONPs					346:351	ZnONPs	346:351	ZnONPs	346:351	In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated.
34333003	1	47	theme	keratin/poly	222:233	arg1	CSZ					355:357	CSZ	355:357	CSZ	355:357	In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated.
34333003	1	47	theme	keratin/poly	222:233	arg1	nanoparticles					331:343	poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles	193:343	poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination	193:370	In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated.
34333003	6	48	theme	higher	1201:1206	arg1	temperatures					1208:1219	higher temperatures	1201:1219	higher temperatures	1201:1219	DSC analysis for K/PVA/CSZ(1:2)-PLA/CSZ(1:2) structure exhibited that the peaks related to the melting points of PLA and PVA shifted to higher temperatures.
34333003	1	49	theme	nanofibrillated	295:309	arg1	/ZnO					326:329	natural nanofibrillated chitosan (CHNF)/ZnO	287:329	natural nanofibrillated chitosan (CHNF)/ZnO	287:329	In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated.
34333003	2	50	from	K/PVA	459:463	arg1	solution					429:436	PLA solution	425:436	PLA solution from one syringe and K/PVA from another one with incorporation of CHNF (CS), CSZ (2:1), (1:1) and (1:2)	425:540	PLA solution from one syringe and K/PVA from another one with incorporation of CHNF (CS), CSZ (2:1), (1:1) and (1:2) were electrospun and produced nanofibers were formed on the rotating collector.
34333003	7	51	theme	2:1	1242:1244	arg1	nanofiber					1260:1268	K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber	1232:1268	K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa	1232:1363	Overally, K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa is suggested as a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation.
34333003	7	51	theme	2:1	1242:1244	arg1	scaffold					1404:1411	a proper wound healing scaffold	1381:1411	a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation	1381:1494	Overally, K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa is suggested as a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation.
34333003	2	52	theme	PLA	425:427	arg1	solution					429:436	PLA solution	425:436	PLA solution from one syringe and K/PVA from another one with incorporation of CHNF (CS), CSZ (2:1), (1:1) and (1:2)	425:540	PLA solution from one syringe and K/PVA from another one with incorporation of CHNF (CS), CSZ (2:1), (1:1) and (1:2) were electrospun and produced nanofibers were formed on the rotating collector.
34333003	1	53	theme	chitosan	311:318	arg1	/ZnO					326:329	natural nanofibrillated chitosan (CHNF)/ZnO	287:329	natural nanofibrillated chitosan (CHNF)/ZnO	287:329	In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated.
34333003	3	54	dep	CHNF	634:637	arg1	amounts					650:656	amounts	650:656	amounts	650:656	Addition of CHNF and ZnONPs amounts in CSZ combination resulted in reduction of the diameter of nanofibers.
34333003	2	55	from	one	478:480	arg1	syringe					447:453	one syringe	443:453	one syringe	443:453	PLA solution from one syringe and K/PVA from another one with incorporation of CHNF (CS), CSZ (2:1), (1:1) and (1:2) were electrospun and produced nanofibers were formed on the rotating collector.
34333003	2	55	from	one	478:480	arg1	1:1					527:529	1:1	527:529	1:1	527:529	PLA solution from one syringe and K/PVA from another one with incorporation of CHNF (CS), CSZ (2:1), (1:1) and (1:2) were electrospun and produced nanofibers were formed on the rotating collector.
34333003	2	56	from	1:2	537:539	arg1	solution					429:436	PLA solution	425:436	PLA solution from one syringe and K/PVA from another one with incorporation of CHNF (CS), CSZ (2:1), (1:1) and (1:2)	425:540	PLA solution from one syringe and K/PVA from another one with incorporation of CHNF (CS), CSZ (2:1), (1:1) and (1:2) were electrospun and produced nanofibers were formed on the rotating collector.
34333003	1	57	dep	investigated	411:422	arg1	designing					139:147	designing	139:147	designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient	139:399	In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated.
34333003	3	58	theme	CSZ	661:663	arg1	combination					665:675	CSZ combination	661:675	CSZ combination	661:675	Addition of CHNF and ZnONPs amounts in CSZ combination resulted in reduction of the diameter of nanofibers.
34333003	1	59	theme	nanofiller	379:388	arg1	ingredient					390:399	the nanofiller ingredient	375:399	the nanofiller ingredient	375:399	In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated.
34333003	1	59	theme	nanofiller	379:388	arg1	nanofibers					171:180	electrospun composite nanofibers	149:180	electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination	149:370	In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated.
34333003	1	60	theme	electrospun	149:159	arg1	ingredient					390:399	the nanofiller ingredient	375:399	the nanofiller ingredient	375:399	In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated.
34333003	1	60	theme	electrospun	149:159	arg1	nanofibers					171:180	electrospun composite nanofibers	149:180	electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination	149:370	In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated.
34333003	4	61	theme	contact	806:812	arg1	angle					814:818	the contact angle	802:818	the contact angle of about 43 ± 3°	802:835	The highest hydrophilicity was reported for K/PVA/CS-PLA/CS sample with the contact angle of about 43 ± 3°.
34333003	1	62	theme	composite	161:169	arg1	ingredient					390:399	the nanofiller ingredient	375:399	the nanofiller ingredient	375:399	In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated.
34333003	1	62	theme	composite	161:169	arg1	nanofibers					171:180	electrospun composite nanofibers	149:180	electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination	149:370	In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated.
34333003	1	63	theme	vinyl	236:240	arg1	alcohol					242:248	vinyl alcohol	236:248	vinyl alcohol	236:248	In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated.
34333003	1	63	theme	vinyl	236:240	arg1	keratin/poly					222:233	keratin/poly	222:233	keratin/poly (vinyl alcohol)	222:249	In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated.
34333003	0	64	theme	nanofibrillated	80:94	arg1	nanoparticles					109:121	nanofibrillated chitosan/ZnO nanoparticles	80:121	nanofibrillated chitosan/ZnO nanoparticles	80:121	Design and characterization of keratin/PVA-PLA nanofibers containing hybrids of nanofibrillated chitosan/ZnO nanoparticles.
34333003	4	65	with	sample	790:795	arg1	angle					814:818	the contact angle	802:818	the contact angle of about 43 ± 3°	802:835	The highest hydrophilicity was reported for K/PVA/CS-PLA/CS sample with the contact angle of about 43 ± 3°.
34333003	7	66	theme	0.96 ± 0.18 MPa	1349:1363	arg1	strength					1337:1344	tensile strength	1329:1344	tensile strength of 0.96 ± 0.18 MPa	1329:1363	Overally, K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa is suggested as a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation.
34333003	7	66	theme	0.96 ± 0.18 MPa	1349:1363	arg1	352.50 ± 31 nm					1287:1300	352.50 ± 31 nm	1287:1300	352.50 ± 31 nm	1287:1300	Overally, K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa is suggested as a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation.
34333003	6	67	theme	PVA	1186:1188	arg1	points					1168:1173	the melting points	1156:1173	the melting points of PLA and PVA	1156:1188	DSC analysis for K/PVA/CSZ(1:2)-PLA/CSZ(1:2) structure exhibited that the peaks related to the melting points of PLA and PVA shifted to higher temperatures.
34333003	1	68	theme	CHNF	321:324	arg1	/ZnO					326:329	natural nanofibrillated chitosan (CHNF)/ZnO	287:329	natural nanofibrillated chitosan (CHNF)/ZnO	287:329	In this paper, designing electrospun composite nanofibers containing poly (lactic acid) (PLA) and keratin/poly (vinyl alcohol) (K/PVA) as the major components and natural nanofibrillated chitosan (CHNF)/ZnO nanoparticles (ZnONPs) (CSZ) combination as the nanofiller ingredient, has been investigated.
34333003	7	69	theme	contact	1303:1309	arg1	352.50 ± 31 nm					1287:1300	352.50 ± 31 nm	1287:1300	352.50 ± 31 nm	1287:1300	Overally, K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa is suggested as a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation.
34333003	7	69	theme	contact	1303:1309	arg1	angle					1311:1315	contact angle	1303:1315	contact angle of 48 ± 3°	1303:1326	Overally, K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa is suggested as a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation.
34333003	7	70	theme	wound	1390:1394	arg1	healing					1396:1402	proper wound healing	1383:1402	a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation	1381:1494	Overally, K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa is suggested as a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation.
34333003	7	71	theme	healing	1396:1402	arg1	nanofiber					1260:1268	K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber	1232:1268	K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa	1232:1363	Overally, K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa is suggested as a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation.
34333003	7	71	theme	healing	1396:1402	arg1	scaffold					1404:1411	a proper wound healing scaffold	1381:1411	a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation	1381:1494	Overally, K/PVA/CSZ(2:1)-PLA/CSZ(2:1) nanofiber with diameter of 352.50 ± 31 nm, contact angle of 48 ± 3°, tensile strength of 0.96 ± 0.18 MPa is suggested as a proper wound healing scaffold that has highest antibacterial as well as potential to increase cell proliferation.
32800542	8	0	theme	main	1177:1180	arg1	Ti					1219:1220	Ti	1219:1220	Ti	1219:1220	The surface of NGF-CS/HA coating with the 65.4 ± 6.4 μm thickness presented a porous network, and the main components of NGF-CS/HA coating were Ti and HA, and maintained the activity and release of NGF.
32800542	8	0	theme	main	1177:1180	arg1	components					1182:1191	the main components	1173:1191	the main components of NGF-CS/HA coating	1173:1212	The surface of NGF-CS/HA coating with the 65.4 ± 6.4 μm thickness presented a porous network, and the main components of NGF-CS/HA coating were Ti and HA, and maintained the activity and release of NGF.
32800542	6	1	theme	red	918:920	arg1	staining					924:931	oil red O staining	914:931	oil red O staining	914:931	Besides, the identification of bone marrow mesenchymal stem cells (BMSCs) was conducted through alizarin red staining, oil red O staining and fluorescence detection.
32800542	5	2	dep	morphology	599:608	arg1	the					587:589	the	587:589	the	587:589	We also observed the surface morphology, thickness, surface adhesion and component analysis of NGF-CS/HA-coating composite titanium by scanning electron microscope, and the release of NGF was also identified via ELISA assay.
32800542	10	3	theme	promoting	1605:1613	arg1	effects					1615:1621	significant promoting effects	1593:1621	significant promoting effects	1593:1621	In conclusion, NGF-CS/HA-coating composite titanium has significant promoting effects on the differentiation of BMSCs into osteoblast and neural cells.
32800542	5	4	theme	NGF-CS/HA-coating	665:681	arg1	titanium					693:700	NGF-CS/HA-coating composite titanium	665:700	NGF-CS/HA-coating composite titanium	665:700	We also observed the surface morphology, thickness, surface adhesion and component analysis of NGF-CS/HA-coating composite titanium by scanning electron microscope, and the release of NGF was also identified via ELISA assay.
32800542	6	5	theme	marrow	831:836	arg1	identification					808:821	the identification	804:821	the identification of bone marrow mesenchymal stem cells (BMSCs)	804:867	Besides, the identification of bone marrow mesenchymal stem cells (BMSCs) was conducted through alizarin red staining, oil red O staining and fluorescence detection.
32800542	2	6	theme	implantology	331:342	arg1	study					317:321	the study	313:321	the study of oral implantology	313:342	Currently, the modification in titanium surface properties has become a hot topic in the study of oral implantology.
32800542	9	7	theme	osteogenesis	1442:1453	arg1	differentiation					1455:1469	the osteogenesis differentiation and neuronal differentiation-related genes and proteins	1438:1525	differentiation	1455:1469	Besides, we successfully obtained and identified BMSCs, and proved that NGF-CS/HA-coating composite titanium could notably upregulated the expression levels of the osteogenesis differentiation and neuronal differentiation-related genes and proteins in BMSCs.
32800542	10	8	theme	BMSCs	1649:1653	arg1	differentiation					1630:1644	the differentiation	1626:1644	the differentiation of BMSCs into osteoblast and neural cells	1626:1686	In conclusion, NGF-CS/HA-coating composite titanium has significant promoting effects on the differentiation of BMSCs into osteoblast and neural cells.
32800542	5	9	theme	titanium	693:700	arg1	thickness					611:619	thickness	611:619	thickness	611:619	We also observed the surface morphology, thickness, surface adhesion and component analysis of NGF-CS/HA-coating composite titanium by scanning electron microscope, and the release of NGF was also identified via ELISA assay.
32800542	5	9	theme	titanium	693:700	arg1	adhesion					630:637	surface adhesion	622:637	surface adhesion	622:637	We also observed the surface morphology, thickness, surface adhesion and component analysis of NGF-CS/HA-coating composite titanium by scanning electron microscope, and the release of NGF was also identified via ELISA assay.
32800542	5	9	theme	titanium	693:700	arg1	morphology					599:608	surface morphology	591:608	surface morphology	591:608	We also observed the surface morphology, thickness, surface adhesion and component analysis of NGF-CS/HA-coating composite titanium by scanning electron microscope, and the release of NGF was also identified via ELISA assay.
32800542	5	9	theme	titanium	693:700	arg1	analysis					653:660	component analysis	643:660	component analysis	643:660	We also observed the surface morphology, thickness, surface adhesion and component analysis of NGF-CS/HA-coating composite titanium by scanning electron microscope, and the release of NGF was also identified via ELISA assay.
32800542	8	10	theme	coating	1206:1212	arg1	Ti					1219:1220	Ti	1219:1220	Ti	1219:1220	The surface of NGF-CS/HA coating with the 65.4 ± 6.4 μm thickness presented a porous network, and the main components of NGF-CS/HA coating were Ti and HA, and maintained the activity and release of NGF.
32800542	8	10	theme	coating	1206:1212	arg1	components					1182:1191	the main components	1173:1191	the main components of NGF-CS/HA coating	1173:1212	The surface of NGF-CS/HA coating with the 65.4 ± 6.4 μm thickness presented a porous network, and the main components of NGF-CS/HA coating were Ti and HA, and maintained the activity and release of NGF.
32800542	6	11	theme	red	900:902	arg1	staining					904:911	alizarin red staining	891:911	alizarin red staining	891:911	Besides, the identification of bone marrow mesenchymal stem cells (BMSCs) was conducted through alizarin red staining, oil red O staining and fluorescence detection.
32800542	2	12	theme	surface	268:274	arg1	properties					276:285	titanium surface properties	259:285	titanium surface properties	259:285	Currently, the modification in titanium surface properties has become a hot topic in the study of oral implantology.
32800542	9	13	theme	differentiation-related	1484:1506	arg1	genes					1508:1512	differentiation-related genes	1484:1512	differentiation-related genes	1484:1512	Besides, we successfully obtained and identified BMSCs, and proved that NGF-CS/HA-coating composite titanium could notably upregulated the expression levels of the osteogenesis differentiation and neuronal differentiation-related genes and proteins in BMSCs.
32800542	10	14	theme	neural	1675:1680	arg1	cells					1682:1686	osteoblast and neural cells	1660:1686	cells	1682:1686	In conclusion, NGF-CS/HA-coating composite titanium has significant promoting effects on the differentiation of BMSCs into osteoblast and neural cells.
32800542	10	15	theme	osteoblast	1660:1669	arg1	cells					1682:1686	osteoblast and neural cells	1660:1686	cells	1682:1686	In conclusion, NGF-CS/HA-coating composite titanium has significant promoting effects on the differentiation of BMSCs into osteoblast and neural cells.
32800542	6	16	theme	fluorescence	937:948	arg1	detection					950:958	fluorescence detection	937:958	fluorescence detection	937:958	Besides, the identification of bone marrow mesenchymal stem cells (BMSCs) was conducted through alizarin red staining, oil red O staining and fluorescence detection.
32800542	5	17	theme	electron	714:721	arg1	microscope					723:732	scanning electron microscope	705:732	scanning electron microscope	705:732	We also observed the surface morphology, thickness, surface adhesion and component analysis of NGF-CS/HA-coating composite titanium by scanning electron microscope, and the release of NGF was also identified via ELISA assay.
32800542	5	18	theme	scanning	705:712	arg1	microscope					723:732	scanning electron microscope	705:732	scanning electron microscope	705:732	We also observed the surface morphology, thickness, surface adhesion and component analysis of NGF-CS/HA-coating composite titanium by scanning electron microscope, and the release of NGF was also identified via ELISA assay.
32800542	7	19	theme	neuronal	1002:1009	arg1	genes					1035:1039	neuronal differentiation-related genes	1002:1039	neuronal differentiation-related genes	1002:1039	and the osteogenesis differentiation and neuronal differentiation-related genes were determined by RT-qPCR assay.
32800542	0	20	theme	marrow	77:82	arg1	cells					101:105	bone marrow mesenchymal stem cells	72:105	bone marrow mesenchymal stem cells	72:105	NGF-CS/HA-coating composite titanium facilitates the differentiation of bone marrow mesenchymal stem cells into osteoblast and neural cells.
32800542	4	21	theme	NGF-CS/HA	501:509	arg1	titanium					530:537	the nerve growth factor-chondroitin sulfate/hydroxyapatite (NGF-CS/HA)-coating composite titanium	441:537	the nerve growth factor-chondroitin sulfate/hydroxyapatite (NGF-CS/HA)-coating composite titanium by modified biomimetic method	441:567	We prepared the nerve growth factor-chondroitin sulfate/hydroxyapatite (NGF-CS/HA)-coating composite titanium by modified biomimetic method.
32800542	0	22	theme	stem	96:99	arg1	cells					101:105	bone marrow mesenchymal stem cells	72:105	bone marrow mesenchymal stem cells	72:105	NGF-CS/HA-coating composite titanium facilitates the differentiation of bone marrow mesenchymal stem cells into osteoblast and neural cells.
32800542	1	23	theme	effective	168:176	arg1	way					178:180	the most effective way	159:180	the most effective way to repair the defect or absence of dentition	159:225	Dental implant is the most effective way to repair the defect or absence of dentition.
32800542	1	23	theme	effective	168:176	arg1	implant					148:154	Dental implant	141:154	Dental implant	141:154	Dental implant is the most effective way to repair the defect or absence of dentition.
32800542	4	24	theme	-coating	511:518	arg1	titanium					530:537	the nerve growth factor-chondroitin sulfate/hydroxyapatite (NGF-CS/HA)-coating composite titanium	441:537	the nerve growth factor-chondroitin sulfate/hydroxyapatite (NGF-CS/HA)-coating composite titanium by modified biomimetic method	441:567	We prepared the nerve growth factor-chondroitin sulfate/hydroxyapatite (NGF-CS/HA)-coating composite titanium by modified biomimetic method.
32800542	5	25	theme	NGF	754:756	arg1	release					743:749	the release	739:749	the release of NGF	739:756	We also observed the surface morphology, thickness, surface adhesion and component analysis of NGF-CS/HA-coating composite titanium by scanning electron microscope, and the release of NGF was also identified via ELISA assay.
32800542	4	26	theme	growth	451:456	arg1	titanium					530:537	the nerve growth factor-chondroitin sulfate/hydroxyapatite (NGF-CS/HA)-coating composite titanium	441:537	the nerve growth factor-chondroitin sulfate/hydroxyapatite (NGF-CS/HA)-coating composite titanium by modified biomimetic method	441:567	We prepared the nerve growth factor-chondroitin sulfate/hydroxyapatite (NGF-CS/HA)-coating composite titanium by modified biomimetic method.
32800542	3	27	theme	titanium	368:375	arg1	coating					385:391	more suitable titanium surface coating	354:391	more suitable titanium surface coating	354:391	However, more suitable titanium surface coating still needs to be further explored.
32800542	4	28	theme	sulfate/hydroxyapatite	477:498	arg1	titanium					530:537	the nerve growth factor-chondroitin sulfate/hydroxyapatite (NGF-CS/HA)-coating composite titanium	441:537	the nerve growth factor-chondroitin sulfate/hydroxyapatite (NGF-CS/HA)-coating composite titanium by modified biomimetic method	441:567	We prepared the nerve growth factor-chondroitin sulfate/hydroxyapatite (NGF-CS/HA)-coating composite titanium by modified biomimetic method.
32800542	0	29	theme	NGF-CS/HA-coating	0:16	arg1	titanium					28:35	NGF-CS/HA-coating composite titanium	0:35	NGF-CS/HA-coating composite titanium	0:35	NGF-CS/HA-coating composite titanium facilitates the differentiation of bone marrow mesenchymal stem cells into osteoblast and neural cells.
32800542	8	30	theme	NGF-CS/HA	1090:1098	arg1	coating					1100:1106	NGF-CS/HA coating	1090:1106	NGF-CS/HA coating with the 65.4 ± 6.4 μm thickness	1090:1139	The surface of NGF-CS/HA coating with the 65.4 ± 6.4 μm thickness presented a porous network, and the main components of NGF-CS/HA coating were Ti and HA, and maintained the activity and release of NGF.
32800542	5	31	theme	surface	591:597	arg1	morphology					599:608	surface morphology	591:608	surface morphology	591:608	We also observed the surface morphology, thickness, surface adhesion and component analysis of NGF-CS/HA-coating composite titanium by scanning electron microscope, and the release of NGF was also identified via ELISA assay.
32800542	2	32	from	modification	243:254	arg1	properties					276:285	titanium surface properties	259:285	titanium surface properties	259:285	Currently, the modification in titanium surface properties has become a hot topic in the study of oral implantology.
32800542	10	33	theme	NGF-CS/HA-coating	1552:1568	arg1	titanium					1580:1587	NGF-CS/HA-coating composite titanium	1552:1587	NGF-CS/HA-coating composite titanium	1552:1587	In conclusion, NGF-CS/HA-coating composite titanium has significant promoting effects on the differentiation of BMSCs into osteoblast and neural cells.
32800542	9	34	theme	NGF-CS/HA-coating	1350:1366	arg1	titanium					1378:1385	NGF-CS/HA-coating composite titanium	1350:1385	NGF-CS/HA-coating composite titanium	1350:1385	Besides, we successfully obtained and identified BMSCs, and proved that NGF-CS/HA-coating composite titanium could notably upregulated the expression levels of the osteogenesis differentiation and neuronal differentiation-related genes and proteins in BMSCs.
32800542	8	35	dep	activity	1249:1256	arg1	the					1245:1247	the	1245:1247	the	1245:1247	The surface of NGF-CS/HA coating with the 65.4 ± 6.4 μm thickness presented a porous network, and the main components of NGF-CS/HA coating were Ti and HA, and maintained the activity and release of NGF.
32800542	8	36	theme	65.4 ± 6.4 μm	1117:1129	arg1	thickness					1131:1139	the 65.4 ± 6.4 μm thickness	1113:1139	the 65.4 ± 6.4 μm thickness	1113:1139	The surface of NGF-CS/HA coating with the 65.4 ± 6.4 μm thickness presented a porous network, and the main components of NGF-CS/HA coating were Ti and HA, and maintained the activity and release of NGF.
32800542	10	37	theme	significant	1593:1603	arg1	effects					1615:1621	significant promoting effects	1593:1621	significant promoting effects	1593:1621	In conclusion, NGF-CS/HA-coating composite titanium has significant promoting effects on the differentiation of BMSCs into osteoblast and neural cells.
32800542	2	38	theme	oral	326:329	arg1	implantology					331:342	oral implantology	326:342	oral implantology	326:342	Currently, the modification in titanium surface properties has become a hot topic in the study of oral implantology.
32800542	4	39	theme	modified	542:549	arg1	method					562:567	modified biomimetic method	542:567	modified biomimetic method	542:567	We prepared the nerve growth factor-chondroitin sulfate/hydroxyapatite (NGF-CS/HA)-coating composite titanium by modified biomimetic method.
32800542	1	40	theme	dentition	217:225	arg1	absence					206:212	absence	206:212	absence	206:212	Dental implant is the most effective way to repair the defect or absence of dentition.
32800542	1	40	theme	dentition	217:225	arg1	defect					196:201	defect	196:201	defect	196:201	Dental implant is the most effective way to repair the defect or absence of dentition.
32800542	8	41	theme	porous	1153:1158	arg1	network					1160:1166	a porous network	1151:1166	a porous network	1151:1166	The surface of NGF-CS/HA coating with the 65.4 ± 6.4 μm thickness presented a porous network, and the main components of NGF-CS/HA coating were Ti and HA, and maintained the activity and release of NGF.
32800542	6	42	theme	stem	850:853	arg1	cells					855:859	mesenchymal stem cells	838:859	bone marrow mesenchymal stem cells (BMSCs)	826:867	Besides, the identification of bone marrow mesenchymal stem cells (BMSCs) was conducted through alizarin red staining, oil red O staining and fluorescence detection.
32800542	6	42	theme	stem	850:853	arg1	BMSCs					862:866	BMSCs	862:866	BMSCs	862:866	Besides, the identification of bone marrow mesenchymal stem cells (BMSCs) was conducted through alizarin red staining, oil red O staining and fluorescence detection.
32800542	1	43	dep	defect	196:201	arg1	the					192:194	the	192:194	the	192:194	Dental implant is the most effective way to repair the defect or absence of dentition.
32800542	6	44	theme	O	922:922	arg1	staining					924:931	oil red O staining	914:931	oil red O staining	914:931	Besides, the identification of bone marrow mesenchymal stem cells (BMSCs) was conducted through alizarin red staining, oil red O staining and fluorescence detection.
32800542	5	45	theme	ELISA	782:786	arg1	assay					788:792	ELISA assay	782:792	ELISA assay	782:792	We also observed the surface morphology, thickness, surface adhesion and component analysis of NGF-CS/HA-coating composite titanium by scanning electron microscope, and the release of NGF was also identified via ELISA assay.
32800542	6	46	theme	oil	914:916	arg1	staining					924:931	oil red O staining	914:931	oil red O staining	914:931	Besides, the identification of bone marrow mesenchymal stem cells (BMSCs) was conducted through alizarin red staining, oil red O staining and fluorescence detection.
32800542	5	47	theme	composite	683:691	arg1	titanium					693:700	NGF-CS/HA-coating composite titanium	665:700	NGF-CS/HA-coating composite titanium	665:700	We also observed the surface morphology, thickness, surface adhesion and component analysis of NGF-CS/HA-coating composite titanium by scanning electron microscope, and the release of NGF was also identified via ELISA assay.
32800542	6	48	theme	bone	826:829	arg1	marrow					831:836	bone marrow mesenchymal stem cells (BMSCs)	826:867	bone marrow mesenchymal stem cells (BMSCs)	826:867	Besides, the identification of bone marrow mesenchymal stem cells (BMSCs) was conducted through alizarin red staining, oil red O staining and fluorescence detection.
32800542	9	49	theme	differentiation	1455:1469	arg1	levels					1428:1433	the expression levels	1413:1433	the expression levels of the osteogenesis differentiation and neuronal differentiation-related genes and proteins in BMSCs	1413:1534	Besides, we successfully obtained and identified BMSCs, and proved that NGF-CS/HA-coating composite titanium could notably upregulated the expression levels of the osteogenesis differentiation and neuronal differentiation-related genes and proteins in BMSCs.
32800542	9	50	theme	neuronal	1475:1482	arg1	levels					1428:1433	the expression levels	1413:1433	the expression levels of the osteogenesis differentiation and neuronal differentiation-related genes and proteins in BMSCs	1413:1534	Besides, we successfully obtained and identified BMSCs, and proved that NGF-CS/HA-coating composite titanium could notably upregulated the expression levels of the osteogenesis differentiation and neuronal differentiation-related genes and proteins in BMSCs.
32800542	8	51	theme	NGF-CS/HA	1196:1204	arg1	coating					1206:1212	NGF-CS/HA coating	1196:1212	NGF-CS/HA coating	1196:1212	The surface of NGF-CS/HA coating with the 65.4 ± 6.4 μm thickness presented a porous network, and the main components of NGF-CS/HA coating were Ti and HA, and maintained the activity and release of NGF.
32800542	6	52	theme	alizarin	891:898	arg1	staining					904:911	alizarin red staining	891:911	alizarin red staining	891:911	Besides, the identification of bone marrow mesenchymal stem cells (BMSCs) was conducted through alizarin red staining, oil red O staining and fluorescence detection.
32800542	5	53	theme	component	643:651	arg1	analysis					653:660	component analysis	643:660	component analysis	643:660	We also observed the surface morphology, thickness, surface adhesion and component analysis of NGF-CS/HA-coating composite titanium by scanning electron microscope, and the release of NGF was also identified via ELISA assay.
32800542	8	54	with	coating	1100:1106	arg1	thickness					1131:1139	the 65.4 ± 6.4 μm thickness	1113:1139	the 65.4 ± 6.4 μm thickness	1113:1139	The surface of NGF-CS/HA coating with the 65.4 ± 6.4 μm thickness presented a porous network, and the main components of NGF-CS/HA coating were Ti and HA, and maintained the activity and release of NGF.
32800542	2	55	theme	hot	300:302	arg1	topic					304:308	a hot topic	298:308	a hot topic in the study of oral implantology	298:342	Currently, the modification in titanium surface properties has become a hot topic in the study of oral implantology.
32800542	9	56	theme	proteins	1518:1525	arg1	levels					1428:1433	the expression levels	1413:1433	the expression levels of the osteogenesis differentiation and neuronal differentiation-related genes and proteins in BMSCs	1413:1534	Besides, we successfully obtained and identified BMSCs, and proved that NGF-CS/HA-coating composite titanium could notably upregulated the expression levels of the osteogenesis differentiation and neuronal differentiation-related genes and proteins in BMSCs.
32800542	6	57	dep	marrow	831:836	arg1	cells					855:859	mesenchymal stem cells	838:859	bone marrow mesenchymal stem cells (BMSCs)	826:867	Besides, the identification of bone marrow mesenchymal stem cells (BMSCs) was conducted through alizarin red staining, oil red O staining and fluorescence detection.
32800542	6	57	dep	marrow	831:836	arg1	BMSCs					862:866	BMSCs	862:866	BMSCs	862:866	Besides, the identification of bone marrow mesenchymal stem cells (BMSCs) was conducted through alizarin red staining, oil red O staining and fluorescence detection.
32800542	8	58	theme	NGF	1273:1275	arg1	release					1262:1268	release	1262:1268	release	1262:1268	The surface of NGF-CS/HA coating with the 65.4 ± 6.4 μm thickness presented a porous network, and the main components of NGF-CS/HA coating were Ti and HA, and maintained the activity and release of NGF.
32800542	8	58	theme	NGF	1273:1275	arg1	activity					1249:1256	activity	1249:1256	activity	1249:1256	The surface of NGF-CS/HA coating with the 65.4 ± 6.4 μm thickness presented a porous network, and the main components of NGF-CS/HA coating were Ti and HA, and maintained the activity and release of NGF.
32800542	4	59	theme	nerve	445:449	arg1	titanium					530:537	the nerve growth factor-chondroitin sulfate/hydroxyapatite (NGF-CS/HA)-coating composite titanium	441:537	the nerve growth factor-chondroitin sulfate/hydroxyapatite (NGF-CS/HA)-coating composite titanium by modified biomimetic method	441:567	We prepared the nerve growth factor-chondroitin sulfate/hydroxyapatite (NGF-CS/HA)-coating composite titanium by modified biomimetic method.
32800542	9	60	dep	differentiation	1455:1469	arg1	genes					1508:1512	differentiation-related genes	1484:1512	differentiation-related genes	1484:1512	Besides, we successfully obtained and identified BMSCs, and proved that NGF-CS/HA-coating composite titanium could notably upregulated the expression levels of the osteogenesis differentiation and neuronal differentiation-related genes and proteins in BMSCs.
32800542	7	61	theme	RT-qPCR	1060:1066	arg1	assay					1068:1072	RT-qPCR assay	1060:1072	RT-qPCR assay	1060:1072	and the osteogenesis differentiation and neuronal differentiation-related genes were determined by RT-qPCR assay.
32800542	2	62	theme	titanium	259:266	arg1	properties					276:285	titanium surface properties	259:285	titanium surface properties	259:285	Currently, the modification in titanium surface properties has become a hot topic in the study of oral implantology.
32800542	1	63	theme	Dental	141:146	arg1	way					178:180	the most effective way	159:180	the most effective way to repair the defect or absence of dentition	159:225	Dental implant is the most effective way to repair the defect or absence of dentition.
32800542	1	63	theme	Dental	141:146	arg1	implant					148:154	Dental implant	141:154	Dental implant	141:154	Dental implant is the most effective way to repair the defect or absence of dentition.
32800542	0	64	theme	mesenchymal	84:94	arg1	cells					101:105	bone marrow mesenchymal stem cells	72:105	bone marrow mesenchymal stem cells	72:105	NGF-CS/HA-coating composite titanium facilitates the differentiation of bone marrow mesenchymal stem cells into osteoblast and neural cells.
32800542	7	65	theme	differentiation-related	1011:1033	arg1	genes					1035:1039	neuronal differentiation-related genes	1002:1039	neuronal differentiation-related genes	1002:1039	and the osteogenesis differentiation and neuronal differentiation-related genes were determined by RT-qPCR assay.
32800542	0	66	theme	cells	101:105	arg1	differentiation					53:67	the differentiation	49:67	the differentiation of bone marrow mesenchymal stem cells into osteoblast and neural cells	49:138	NGF-CS/HA-coating composite titanium facilitates the differentiation of bone marrow mesenchymal stem cells into osteoblast and neural cells.
32800542	10	67	contain	has	1589:1591	arg1	titanium					1580:1587	NGF-CS/HA-coating composite titanium	1552:1587	NGF-CS/HA-coating composite titanium	1552:1587	In conclusion, NGF-CS/HA-coating composite titanium has significant promoting effects on the differentiation of BMSCs into osteoblast and neural cells.
32800542	10	67	contain	has	1589:1591	arg2	effects					1615:1621	significant promoting effects	1593:1621	significant promoting effects	1593:1621	In conclusion, NGF-CS/HA-coating composite titanium has significant promoting effects on the differentiation of BMSCs into osteoblast and neural cells.
32800542	4	68	theme	composite	520:528	arg1	titanium					530:537	the nerve growth factor-chondroitin sulfate/hydroxyapatite (NGF-CS/HA)-coating composite titanium	441:537	the nerve growth factor-chondroitin sulfate/hydroxyapatite (NGF-CS/HA)-coating composite titanium by modified biomimetic method	441:567	We prepared the nerve growth factor-chondroitin sulfate/hydroxyapatite (NGF-CS/HA)-coating composite titanium by modified biomimetic method.
32800542	0	69	theme	osteoblast	112:121	arg1	cells					134:138	osteoblast and neural cells	112:138	cells	134:138	NGF-CS/HA-coating composite titanium facilitates the differentiation of bone marrow mesenchymal stem cells into osteoblast and neural cells.
32800542	7	70	theme	osteogenesis	969:980	arg1	differentiation					982:996	the osteogenesis differentiation	965:996	the osteogenesis differentiation	965:996	and the osteogenesis differentiation and neuronal differentiation-related genes were determined by RT-qPCR assay.
32800542	2	71	from	topic	304:308	arg1	study					317:321	the study	313:321	the study of oral implantology	313:342	Currently, the modification in titanium surface properties has become a hot topic in the study of oral implantology.
32800542	4	72	theme	factor-chondroitin	458:475	arg1	titanium					530:537	the nerve growth factor-chondroitin sulfate/hydroxyapatite (NGF-CS/HA)-coating composite titanium	441:537	the nerve growth factor-chondroitin sulfate/hydroxyapatite (NGF-CS/HA)-coating composite titanium by modified biomimetic method	441:567	We prepared the nerve growth factor-chondroitin sulfate/hydroxyapatite (NGF-CS/HA)-coating composite titanium by modified biomimetic method.
32800542	0	73	theme	neural	127:132	arg1	cells					134:138	osteoblast and neural cells	112:138	cells	134:138	NGF-CS/HA-coating composite titanium facilitates the differentiation of bone marrow mesenchymal stem cells into osteoblast and neural cells.
32800542	5	74	theme	surface	622:628	arg1	adhesion					630:637	surface adhesion	622:637	surface adhesion	622:637	We also observed the surface morphology, thickness, surface adhesion and component analysis of NGF-CS/HA-coating composite titanium by scanning electron microscope, and the release of NGF was also identified via ELISA assay.
32800542	0	75	theme	composite	18:26	arg1	titanium					28:35	NGF-CS/HA-coating composite titanium	0:35	NGF-CS/HA-coating composite titanium	0:35	NGF-CS/HA-coating composite titanium facilitates the differentiation of bone marrow mesenchymal stem cells into osteoblast and neural cells.
32800542	8	76	theme	coating	1100:1106	arg1	surface					1079:1085	The surface	1075:1085	The surface of NGF-CS/HA coating with the 65.4 ± 6.4 μm thickness	1075:1139	The surface of NGF-CS/HA coating with the 65.4 ± 6.4 μm thickness presented a porous network, and the main components of NGF-CS/HA coating were Ti and HA, and maintained the activity and release of NGF.
32800542	3	77	theme	surface	377:383	arg1	coating					385:391	more suitable titanium surface coating	354:391	more suitable titanium surface coating	354:391	However, more suitable titanium surface coating still needs to be further explored.
32800542	3	78	theme	suitable	359:366	arg1	coating					385:391	more suitable titanium surface coating	354:391	more suitable titanium surface coating	354:391	However, more suitable titanium surface coating still needs to be further explored.
32800542	9	79	theme	composite	1368:1376	arg1	titanium					1378:1385	NGF-CS/HA-coating composite titanium	1350:1385	NGF-CS/HA-coating composite titanium	1350:1385	Besides, we successfully obtained and identified BMSCs, and proved that NGF-CS/HA-coating composite titanium could notably upregulated the expression levels of the osteogenesis differentiation and neuronal differentiation-related genes and proteins in BMSCs.
32800542	0	80	theme	bone	72:75	arg1	cells					101:105	bone marrow mesenchymal stem cells	72:105	bone marrow mesenchymal stem cells	72:105	NGF-CS/HA-coating composite titanium facilitates the differentiation of bone marrow mesenchymal stem cells into osteoblast and neural cells.
32800542	9	81	from	levels	1428:1433	arg1	BMSCs					1530:1534	BMSCs	1530:1534	BMSCs	1530:1534	Besides, we successfully obtained and identified BMSCs, and proved that NGF-CS/HA-coating composite titanium could notably upregulated the expression levels of the osteogenesis differentiation and neuronal differentiation-related genes and proteins in BMSCs.
32800542	4	82	theme	biomimetic	551:560	arg1	method					562:567	modified biomimetic method	542:567	modified biomimetic method	542:567	We prepared the nerve growth factor-chondroitin sulfate/hydroxyapatite (NGF-CS/HA)-coating composite titanium by modified biomimetic method.
32800542	10	83	theme	composite	1570:1578	arg1	titanium					1580:1587	NGF-CS/HA-coating composite titanium	1552:1587	NGF-CS/HA-coating composite titanium	1552:1587	In conclusion, NGF-CS/HA-coating composite titanium has significant promoting effects on the differentiation of BMSCs into osteoblast and neural cells.
32800542	9	84	theme	expression	1417:1426	arg1	levels					1428:1433	the expression levels	1413:1433	the expression levels of the osteogenesis differentiation and neuronal differentiation-related genes and proteins in BMSCs	1413:1534	Besides, we successfully obtained and identified BMSCs, and proved that NGF-CS/HA-coating composite titanium could notably upregulated the expression levels of the osteogenesis differentiation and neuronal differentiation-related genes and proteins in BMSCs.
32800542	6	85	theme	mesenchymal	838:848	arg1	cells					855:859	mesenchymal stem cells	838:859	bone marrow mesenchymal stem cells (BMSCs)	826:867	Besides, the identification of bone marrow mesenchymal stem cells (BMSCs) was conducted through alizarin red staining, oil red O staining and fluorescence detection.
32800542	6	85	theme	mesenchymal	838:848	arg1	BMSCs					862:866	BMSCs	862:866	BMSCs	862:866	Besides, the identification of bone marrow mesenchymal stem cells (BMSCs) was conducted through alizarin red staining, oil red O staining and fluorescence detection.
34212398	0	0	from	expression	67:76	arg1	mice					119:122	mice	119:122	mice fed high-fat diets with distinct 18-carbon fatty acid composition	119:188	Effect of lipopolysaccharide on peripheral tissue and hypothalamic expression of metabolic and inflammatory markers in mice fed high-fat diets with distinct 18-carbon fatty acid composition.
34212398	8	1	theme	mRNA	1524:1527	arg1	expression					1529:1538	pro-inflammatory cytokine mRNA expression	1498:1538	pro-inflammatory cytokine mRNA expression	1498:1538	LPS challenge increased pro-inflammatory cytokine mRNA expression in the brain and peripheral tissues.
34212398	3	2	theme	muscle	731:736	arg1	tissues					738:744	muscle tissues	731:744	muscle tissues	731:744	We conducted this study to test whether diets with distinct 18-carbon fatty acid differentially affect inflammatory and metabolic response to LPS exposure in the hypothalamus, liver, and muscle tissues.
34212398	5	3	from	%	965:965	arg1	fat					944:946	5.6% kcal fat	934:946	5.6% kcal fat from lard and 4.4% kcal fat from soybean oil	934:991	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	1	4	theme	saturation	276:285	arg1	degree					266:271	their degree	260:271	their degree of saturation and chain length	260:302	Physiological and metabolic effects of fatty acids are determined by their degree of saturation and chain length.
34212398	4	5	theme	lipopolysaccharide	834:851	arg1	injection					859:867	a terminal 4-h lipopolysaccharide (LPS) injection	819:867	a terminal 4-h lipopolysaccharide (LPS) injection	819:867	Four experimental diets were fed for 4 weeks to male C57BL/6J mice, and a terminal 4-h lipopolysaccharide (LPS) injection was administered.
34212398	3	6	from	response	674:681	arg1	liver					720:724	liver	720:724	liver	720:724	We conducted this study to test whether diets with distinct 18-carbon fatty acid differentially affect inflammatory and metabolic response to LPS exposure in the hypothalamus, liver, and muscle tissues.
34212398	3	6	from	response	674:681	arg1	hypothalamus					706:717	hypothalamus	706:717	hypothalamus	706:717	We conducted this study to test whether diets with distinct 18-carbon fatty acid differentially affect inflammatory and metabolic response to LPS exposure in the hypothalamus, liver, and muscle tissues.
34212398	3	6	from	response	674:681	arg1	tissues					738:744	muscle tissues	731:744	muscle tissues	731:744	We conducted this study to test whether diets with distinct 18-carbon fatty acid differentially affect inflammatory and metabolic response to LPS exposure in the hypothalamus, liver, and muscle tissues.
34212398	5	7	from	butter	1093:1098	arg1	oil					1203:1205	soybean oil	1195:1205	soybean oil (SBO; polyunsaturated fatty acid-rich fat)	1195:1248	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	5	7	from	butter	1093:1098	arg1	oil					1144:1146	olive oil	1138:1146	olive oil (OLO; monounsaturated fatty acid-rich oil)	1138:1189	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	5	7	from	butter	1093:1098	arg1	SBO					1208:1210	SBO	1208:1210	SBO	1208:1210	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	5	7	from	butter	1093:1098	arg1	OLO					1149:1151	OLO	1149:1151	OLO; monounsaturated fatty acid-rich oil	1149:1188	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	5	7	from	butter	1093:1098	arg1	lard					1054:1057	lard	1054:1057	lard	1054:1057	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	5	7	from	butter	1093:1098	arg1	fat					1072:1074	20% kcal fat	1063:1074	20% kcal fat	1063:1074	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	2	8	theme	18-carbon	316:324	arg1	acids					332:336	18-carbon fatty acids	316:336	18-carbon fatty acids with various degrees of saturation	316:371	Effects of 18-carbon fatty acids with various degrees of saturation on inflammatory, oxidative, and neuropeptide gene transcription, especially in the hypothalamus, in response to LPS-induced acute inflammation have not been well studied.
34212398	8	9	theme	peripheral	1557:1566	arg1	tissues					1568:1574	peripheral tissues	1557:1574	peripheral tissues	1557:1574	LPS challenge increased pro-inflammatory cytokine mRNA expression in the brain and peripheral tissues.
34212398	7	10	contain	had	1345:1347	arg2	expression					1362:1371	lowest Cpt1b expression	1349:1371	lowest Cpt1b expression in the liver	1349:1384	The SBO group had lowest Cpt1b expression in the liver, and OLO group had the lowest Pomc and the highest Lepr expression in the hypothalamus.
34212398	7	10	contain	had	1345:1347	arg1	group					1339:1343	The SBO group	1331:1343	The SBO group	1331:1343	The SBO group had lowest Cpt1b expression in the liver, and OLO group had the lowest Pomc and the highest Lepr expression in the hypothalamus.
34212398	10	11	theme	18-carbon	1705:1713	arg1	acids					1721:1725	18-carbon fatty acids	1705:1725	18-carbon fatty acids	1705:1725	Therefore, saturation degree of 18-carbon fatty acids may not play a critical role in their effects on inflammatory and metabolic indicators in response to acute inflammation induced by LPS.
34212398	10	12	theme	acute	1829:1833	arg1	inflammation					1835:1846	acute inflammation	1829:1846	acute inflammation induced by LPS	1829:1861	Therefore, saturation degree of 18-carbon fatty acids may not play a critical role in their effects on inflammatory and metabolic indicators in response to acute inflammation induced by LPS.
34212398	3	13	theme	18-carbon	604:612	arg1	acid					620:623	distinct 18-carbon fatty acid	595:623	distinct 18-carbon fatty acid	595:623	We conducted this study to test whether diets with distinct 18-carbon fatty acid differentially affect inflammatory and metabolic response to LPS exposure in the hypothalamus, liver, and muscle tissues.
34212398	1	14	theme	fatty	230:234	arg1	acids					236:240	fatty acids	230:240	fatty acids	230:240	Physiological and metabolic effects of fatty acids are determined by their degree of saturation and chain length.
34212398	7	15	theme	highest	1429:1435	arg1	expression					1442:1451	Lepr expression	1437:1451	the highest Lepr expression in the hypothalamus	1425:1471	The SBO group had lowest Cpt1b expression in the liver, and OLO group had the lowest Pomc and the highest Lepr expression in the hypothalamus.
34212398	1	16	theme	chain	291:295	arg1	length					297:302	chain length	291:302	chain length	291:302	Physiological and metabolic effects of fatty acids are determined by their degree of saturation and chain length.
34212398	0	17	theme	distinct	148:155	arg1	composition					178:188	distinct 18-carbon fatty acid composition	148:188	distinct 18-carbon fatty acid composition	148:188	Effect of lipopolysaccharide on peripheral tissue and hypothalamic expression of metabolic and inflammatory markers in mice fed high-fat diets with distinct 18-carbon fatty acid composition.
34212398	10	18	dep	inflammation	1835:1846	arg1	response					1817:1824	response	1817:1824	response	1817:1824	Therefore, saturation degree of 18-carbon fatty acids may not play a critical role in their effects on inflammatory and metabolic indicators in response to acute inflammation induced by LPS.
34212398	0	19	with	diets	137:141	arg1	composition					178:188	distinct 18-carbon fatty acid composition	148:188	distinct 18-carbon fatty acid composition	148:188	Effect of lipopolysaccharide on peripheral tissue and hypothalamic expression of metabolic and inflammatory markers in mice fed high-fat diets with distinct 18-carbon fatty acid composition.
34212398	0	20	theme	fatty	167:171	arg1	composition					178:188	distinct 18-carbon fatty acid composition	148:188	distinct 18-carbon fatty acid composition	148:188	Effect of lipopolysaccharide on peripheral tissue and hypothalamic expression of metabolic and inflammatory markers in mice fed high-fat diets with distinct 18-carbon fatty acid composition.
34212398	5	21	dep	SHB	1101:1103	arg1	fat					1132:1134	saturated fatty acid-rich fat	1106:1134	saturated fatty acid-rich fat	1106:1134	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	5	22	theme	fatty	1170:1174	arg1	oil					1186:1188	monounsaturated fatty acid-rich oil	1154:1188	OLO; monounsaturated fatty acid-rich oil	1149:1188	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	8	23	theme	cytokine	1515:1522	arg1	expression					1529:1538	pro-inflammatory cytokine mRNA expression	1498:1538	pro-inflammatory cytokine mRNA expression	1498:1538	LPS challenge increased pro-inflammatory cytokine mRNA expression in the brain and peripheral tissues.
34212398	6	24	theme	HFD-fed	1268:1274	arg1	mice					1276:1279	HFD-fed mice	1268:1279	HFD-fed mice	1268:1279	Compared to CON, HFD-fed mice had higher weight gain and body fat accumulation.
34212398	2	25	theme	acute	497:501	arg1	inflammation					503:514	LPS-induced acute inflammation	485:514	LPS-induced acute inflammation	485:514	Effects of 18-carbon fatty acids with various degrees of saturation on inflammatory, oxidative, and neuropeptide gene transcription, especially in the hypothalamus, in response to LPS-induced acute inflammation have not been well studied.
34212398	1	26	theme	length	297:302	arg1	degree					266:271	their degree	260:271	their degree of saturation and chain length	260:302	Physiological and metabolic effects of fatty acids are determined by their degree of saturation and chain length.
34212398	5	27	theme	olive	1138:1142	arg1	oil					1144:1146	olive oil	1138:1146	olive oil (OLO; monounsaturated fatty acid-rich oil)	1138:1189	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	5	27	theme	olive	1138:1142	arg1	OLO					1149:1151	OLO	1149:1151	OLO; monounsaturated fatty acid-rich oil	1149:1188	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	0	28	theme	metabolic	81:89	arg1	markers					108:114	metabolic and inflammatory markers	81:114	metabolic and inflammatory markers in mice fed high-fat diets with distinct 18-carbon fatty acid composition	81:188	Effect of lipopolysaccharide on peripheral tissue and hypothalamic expression of metabolic and inflammatory markers in mice fed high-fat diets with distinct 18-carbon fatty acid composition.
34212398	7	29	theme	OLO	1391:1393	arg1	group					1395:1399	OLO group	1391:1399	OLO group	1391:1399	The SBO group had lowest Cpt1b expression in the liver, and OLO group had the lowest Pomc and the highest Lepr expression in the hypothalamus.
34212398	0	30	from	Effect	0:5	arg1	tissue					43:48	peripheral tissue	32:48	peripheral tissue	32:48	Effect of lipopolysaccharide on peripheral tissue and hypothalamic expression of metabolic and inflammatory markers in mice fed high-fat diets with distinct 18-carbon fatty acid composition.
34212398	0	30	from	Effect	0:5	arg1	expression					67:76	hypothalamic expression	54:76	hypothalamic expression	54:76	Effect of lipopolysaccharide on peripheral tissue and hypothalamic expression of metabolic and inflammatory markers in mice fed high-fat diets with distinct 18-carbon fatty acid composition.
34212398	0	31	theme	inflammatory	95:106	arg1	markers					108:114	metabolic and inflammatory markers	81:114	metabolic and inflammatory markers in mice fed high-fat diets with distinct 18-carbon fatty acid composition	81:188	Effect of lipopolysaccharide on peripheral tissue and hypothalamic expression of metabolic and inflammatory markers in mice fed high-fat diets with distinct 18-carbon fatty acid composition.
34212398	7	32	dep	Pomc	1416:1419	arg1	the					1405:1407	the	1405:1407	the	1405:1407	The SBO group had lowest Cpt1b expression in the liver, and OLO group had the lowest Pomc and the highest Lepr expression in the hypothalamus.
34212398	5	33	theme	soybean	1195:1201	arg1	SBO					1208:1210	SBO	1208:1210	SBO	1208:1210	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	5	33	theme	soybean	1195:1201	arg1	oil					1203:1205	soybean oil	1195:1205	soybean oil (SBO; polyunsaturated fatty acid-rich fat)	1195:1248	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	5	34	theme	saturated	1106:1114	arg1	fat					1132:1134	saturated fatty acid-rich fat	1106:1134	saturated fatty acid-rich fat	1106:1134	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	5	35	theme	%	1038:1038	arg1	fat					1045:1047	25% kcal fat	1036:1047	25% kcal fat	1036:1047	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	7	36	from	expression	1442:1451	arg1	hypothalamus					1460:1471	the hypothalamus	1456:1471	the hypothalamus	1456:1471	The SBO group had lowest Cpt1b expression in the liver, and OLO group had the lowest Pomc and the highest Lepr expression in the hypothalamus.
34212398	9	37	theme	LPS-induced	1637:1647	arg1	responses					1662:1670	LPS-induced inflammatory responses	1637:1670	LPS-induced inflammatory responses	1637:1670	However, the diets did not exert distinguishable effects on LPS-induced inflammatory responses.
34212398	0	38	theme	lipopolysaccharide	10:27	arg1	Effect					0:5	Effect	0:5	Effect of lipopolysaccharide on peripheral tissue and hypothalamic expression of metabolic and inflammatory markers in mice fed high-fat diets with distinct 18-carbon fatty acid composition.	0:189	Effect of lipopolysaccharide on peripheral tissue and hypothalamic expression of metabolic and inflammatory markers in mice fed high-fat diets with distinct 18-carbon fatty acid composition.
34212398	5	39	theme	shea	1088:1091	arg1	SHB					1101:1103	SHB	1101:1103	SHB	1101:1103	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	5	39	theme	shea	1088:1091	arg1	butter					1093:1098	either shea butter	1081:1098	either shea butter (SHB; saturated fatty acid-rich fat)	1081:1135	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	5	40	theme	%	937:937	arg1	fat					944:946	5.6% kcal fat	934:946	5.6% kcal fat from lard and 4.4% kcal fat from soybean oil	934:991	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	5	41	dep	SBO	1208:1210	arg1	fat					1245:1247	polyunsaturated fatty acid-rich fat	1213:1247	polyunsaturated fatty acid-rich fat	1213:1247	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	2	42	theme	gene	418:421	arg1	transcription					423:435	neuropeptide gene transcription	405:435	neuropeptide gene transcription	405:435	Effects of 18-carbon fatty acids with various degrees of saturation on inflammatory, oxidative, and neuropeptide gene transcription, especially in the hypothalamus, in response to LPS-induced acute inflammation have not been well studied.
34212398	0	43	theme	peripheral	32:41	arg1	tissue					43:48	peripheral tissue	32:48	peripheral tissue	32:48	Effect of lipopolysaccharide on peripheral tissue and hypothalamic expression of metabolic and inflammatory markers in mice fed high-fat diets with distinct 18-carbon fatty acid composition.
34212398	5	44	theme	20	1063:1064	arg1	%					1065:1065	%	1065:1065	%	1065:1065	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	7	45	theme	Cpt1b	1356:1360	arg1	expression					1362:1371	lowest Cpt1b expression	1349:1371	lowest Cpt1b expression in the liver	1349:1384	The SBO group had lowest Cpt1b expression in the liver, and OLO group had the lowest Pomc and the highest Lepr expression in the hypothalamus.
34212398	10	46	theme	saturation	1684:1693	arg1	degree					1695:1700	saturation degree	1684:1700	saturation degree of 18-carbon fatty acids	1684:1725	Therefore, saturation degree of 18-carbon fatty acids may not play a critical role in their effects on inflammatory and metabolic indicators in response to acute inflammation induced by LPS.
34212398	4	47	theme	terminal	821:828	arg1	injection					859:867	a terminal 4-h lipopolysaccharide (LPS) injection	819:867	a terminal 4-h lipopolysaccharide (LPS) injection	819:867	Four experimental diets were fed for 4 weeks to male C57BL/6J mice, and a terminal 4-h lipopolysaccharide (LPS) injection was administered.
34212398	5	48	theme	kcal	1067:1070	arg1	fat					1072:1074	20% kcal fat	1063:1074	20% kcal fat	1063:1074	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	6	49	contain	had	1281:1283	arg1	mice					1276:1279	HFD-fed mice	1268:1279	HFD-fed mice	1268:1279	Compared to CON, HFD-fed mice had higher weight gain and body fat accumulation.
34212398	6	49	contain	had	1281:1283	arg2	accumulation					1317:1328	body fat accumulation	1308:1328	body fat accumulation	1308:1328	Compared to CON, HFD-fed mice had higher weight gain and body fat accumulation.
34212398	6	49	contain	had	1281:1283	arg2	gain					1299:1302	higher weight gain	1285:1302	higher weight gain	1285:1302	Compared to CON, HFD-fed mice had higher weight gain and body fat accumulation.
34212398	4	50	theme	male	795:798	arg1	mice					809:812	male C57BL/6J mice	795:812	male C57BL/6J mice	795:812	Four experimental diets were fed for 4 weeks to male C57BL/6J mice, and a terminal 4-h lipopolysaccharide (LPS) injection was administered.
34212398	1	51	theme	acids	236:240	arg1	effects					219:225	Physiological and metabolic effects	191:225	Physiological and metabolic effects of fatty acids	191:240	Physiological and metabolic effects of fatty acids are determined by their degree of saturation and chain length.
34212398	2	52	dep	inflammation	503:514	arg1	response					473:480	response	473:480	response	473:480	Effects of 18-carbon fatty acids with various degrees of saturation on inflammatory, oxidative, and neuropeptide gene transcription, especially in the hypothalamus, in response to LPS-induced acute inflammation have not been well studied.
34212398	7	53	theme	SBO	1335:1337	arg1	group					1339:1343	The SBO group	1331:1343	The SBO group	1331:1343	The SBO group had lowest Cpt1b expression in the liver, and OLO group had the lowest Pomc and the highest Lepr expression in the hypothalamus.
34212398	9	54	theme	distinguishable	1610:1624	arg1	effects					1626:1632	distinguishable effects	1610:1632	distinguishable effects	1610:1632	However, the diets did not exert distinguishable effects on LPS-induced inflammatory responses.
34212398	3	55	theme	LPS	686:688	arg1	exposure					690:697	LPS exposure	686:697	LPS exposure	686:697	We conducted this study to test whether diets with distinct 18-carbon fatty acid differentially affect inflammatory and metabolic response to LPS exposure in the hypothalamus, liver, and muscle tissues.
34212398	6	56	theme	fat	1313:1315	arg1	accumulation					1317:1328	body fat accumulation	1308:1328	body fat accumulation	1308:1328	Compared to CON, HFD-fed mice had higher weight gain and body fat accumulation.
34212398	5	57	theme	kcal	967:970	arg1	fat					972:974	kcal fat	967:974	kcal fat	967:974	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	10	58	theme	critical	1742:1749	arg1	role					1751:1754	a critical role	1740:1754	a critical role	1740:1754	Therefore, saturation degree of 18-carbon fatty acids may not play a critical role in their effects on inflammatory and metabolic indicators in response to acute inflammation induced by LPS.
34212398	5	59	theme	fatty	1229:1233	arg1	fat					1245:1247	polyunsaturated fatty acid-rich fat	1213:1247	polyunsaturated fatty acid-rich fat	1213:1247	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	2	60	theme	saturation	362:371	arg1	degrees					351:357	various degrees	343:357	various degrees of saturation	343:371	Effects of 18-carbon fatty acids with various degrees of saturation on inflammatory, oxidative, and neuropeptide gene transcription, especially in the hypothalamus, in response to LPS-induced acute inflammation have not been well studied.
34212398	5	61	from	lard	953:956	arg1	fat					944:946	5.6% kcal fat	934:946	5.6% kcal fat from lard and 4.4% kcal fat from soybean oil	934:991	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	6	62	theme	weight	1292:1297	arg1	gain					1299:1302	higher weight gain	1285:1302	higher weight gain	1285:1302	Compared to CON, HFD-fed mice had higher weight gain and body fat accumulation.
34212398	7	63	from	expression	1362:1371	arg1	liver					1380:1384	the liver	1376:1384	the liver	1376:1384	The SBO group had lowest Cpt1b expression in the liver, and OLO group had the lowest Pomc and the highest Lepr expression in the hypothalamus.
34212398	5	64	dep	OLO	1149:1151	arg1	oil					1186:1188	monounsaturated fatty acid-rich oil	1154:1188	OLO; monounsaturated fatty acid-rich oil	1149:1188	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	4	65	theme	4-h	830:832	arg1	LPS					854:856	LPS	854:856	LPS	854:856	Four experimental diets were fed for 4 weeks to male C57BL/6J mice, and a terminal 4-h lipopolysaccharide (LPS) injection was administered.
34212398	4	65	theme	4-h	830:832	arg1	lipopolysaccharide					834:851	4-h lipopolysaccharide	830:851	a terminal 4-h lipopolysaccharide (LPS) injection	819:867	Four experimental diets were fed for 4 weeks to male C57BL/6J mice, and a terminal 4-h lipopolysaccharide (LPS) injection was administered.
34212398	2	66	theme	fatty	326:330	arg1	acids					332:336	18-carbon fatty acids	316:336	18-carbon fatty acids with various degrees of saturation	316:371	Effects of 18-carbon fatty acids with various degrees of saturation on inflammatory, oxidative, and neuropeptide gene transcription, especially in the hypothalamus, in response to LPS-induced acute inflammation have not been well studied.
34212398	1	67	theme	Physiological	191:203	arg1	effects					219:225	Physiological and metabolic effects	191:225	Physiological and metabolic effects of fatty acids	191:240	Physiological and metabolic effects of fatty acids are determined by their degree of saturation and chain length.
34212398	2	68	from	inflammatory	376:387	arg1	hypothalamus					456:467	the hypothalamus	452:467	the hypothalamus	452:467	Effects of 18-carbon fatty acids with various degrees of saturation on inflammatory, oxidative, and neuropeptide gene transcription, especially in the hypothalamus, in response to LPS-induced acute inflammation have not been well studied.
34212398	10	69	theme	metabolic	1793:1801	arg1	indicators					1803:1812	inflammatory and metabolic indicators	1776:1812	inflammatory and metabolic indicators	1776:1812	Therefore, saturation degree of 18-carbon fatty acids may not play a critical role in their effects on inflammatory and metabolic indicators in response to acute inflammation induced by LPS.
34212398	0	70	theme	high-fat	128:135	arg1	diets					137:141	high-fat diets	128:141	high-fat diets with distinct 18-carbon fatty acid composition	128:188	Effect of lipopolysaccharide on peripheral tissue and hypothalamic expression of metabolic and inflammatory markers in mice fed high-fat diets with distinct 18-carbon fatty acid composition.
34212398	5	71	from	oil	989:991	arg1	fat					944:946	5.6% kcal fat	934:946	5.6% kcal fat from lard and 4.4% kcal fat from soybean oil	934:991	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	1	72	theme	metabolic	209:217	arg1	effects					219:225	Physiological and metabolic effects	191:225	Physiological and metabolic effects of fatty acids	191:240	Physiological and metabolic effects of fatty acids are determined by their degree of saturation and chain length.
34212398	7	73	theme	Lepr	1437:1440	arg1	expression					1442:1451	Lepr expression	1437:1451	the highest Lepr expression in the hypothalamus	1425:1471	The SBO group had lowest Cpt1b expression in the liver, and OLO group had the lowest Pomc and the highest Lepr expression in the hypothalamus.
34212398	5	74	contain	containing	923:932	arg1	diet					912:915	a control diet	902:915	a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil	902:991	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	5	74	contain	containing	923:932	arg2	fat					944:946	5.6% kcal fat	934:946	5.6% kcal fat from lard and 4.4% kcal fat from soybean oil	934:991	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	5	74	contain	containing	923:932	arg1	HFD					1020:1022	HFD	1020:1022	HFD	1020:1022	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	5	74	contain	containing	923:932	arg1	CON					918:920	CON	918:920	CON	918:920	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	10	75	theme	inflammatory	1776:1787	arg1	indicators					1803:1812	inflammatory and metabolic indicators	1776:1812	inflammatory and metabolic indicators	1776:1812	Therefore, saturation degree of 18-carbon fatty acids may not play a critical role in their effects on inflammatory and metabolic indicators in response to acute inflammation induced by LPS.
34212398	3	76	theme	distinct	595:602	arg1	acid					620:623	distinct 18-carbon fatty acid	595:623	distinct 18-carbon fatty acid	595:623	We conducted this study to test whether diets with distinct 18-carbon fatty acid differentially affect inflammatory and metabolic response to LPS exposure in the hypothalamus, liver, and muscle tissues.
34212398	3	77	with	diets	584:588	arg1	acid					620:623	distinct 18-carbon fatty acid	595:623	distinct 18-carbon fatty acid	595:623	We conducted this study to test whether diets with distinct 18-carbon fatty acid differentially affect inflammatory and metabolic response to LPS exposure in the hypothalamus, liver, and muscle tissues.
34212398	0	78	theme	18-carbon	157:165	arg1	composition					178:188	distinct 18-carbon fatty acid composition	148:188	distinct 18-carbon fatty acid composition	148:188	Effect of lipopolysaccharide on peripheral tissue and hypothalamic expression of metabolic and inflammatory markers in mice fed high-fat diets with distinct 18-carbon fatty acid composition.
34212398	5	79	theme	control	904:910	arg1	diet					912:915	a control diet	902:915	a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil	902:991	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	5	79	theme	control	904:910	arg1	HFD					1020:1022	HFD	1020:1022	HFD	1020:1022	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	5	79	theme	control	904:910	arg1	CON					918:920	CON	918:920	CON	918:920	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	10	80	from	effects	1765:1771	arg1	indicators					1803:1812	inflammatory and metabolic indicators	1776:1812	inflammatory and metabolic indicators	1776:1812	Therefore, saturation degree of 18-carbon fatty acids may not play a critical role in their effects on inflammatory and metabolic indicators in response to acute inflammation induced by LPS.
34212398	0	81	from	mice	119:122	arg1	tissue					43:48	peripheral tissue	32:48	peripheral tissue	32:48	Effect of lipopolysaccharide on peripheral tissue and hypothalamic expression of metabolic and inflammatory markers in mice fed high-fat diets with distinct 18-carbon fatty acid composition.
34212398	0	81	from	mice	119:122	arg1	expression					67:76	hypothalamic expression	54:76	hypothalamic expression	54:76	Effect of lipopolysaccharide on peripheral tissue and hypothalamic expression of metabolic and inflammatory markers in mice fed high-fat diets with distinct 18-carbon fatty acid composition.
34212398	5	82	dep	lard	953:956	arg1	fat					972:974	kcal fat	967:974	kcal fat	967:974	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	0	83	theme	acid	173:176	arg1	composition					178:188	distinct 18-carbon fatty acid composition	148:188	distinct 18-carbon fatty acid composition	148:188	Effect of lipopolysaccharide on peripheral tissue and hypothalamic expression of metabolic and inflammatory markers in mice fed high-fat diets with distinct 18-carbon fatty acid composition.
34212398	0	84	from	markers	108:114	arg1	mice					119:122	mice	119:122	mice fed high-fat diets with distinct 18-carbon fatty acid composition	119:188	Effect of lipopolysaccharide on peripheral tissue and hypothalamic expression of metabolic and inflammatory markers in mice fed high-fat diets with distinct 18-carbon fatty acid composition.
34212398	5	85	theme	monounsaturated	1154:1168	arg1	oil					1186:1188	monounsaturated fatty acid-rich oil	1154:1188	OLO; monounsaturated fatty acid-rich oil	1149:1188	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	5	86	theme	high-fat	1004:1011	arg1	diets					1013:1017	three high-fat diets	998:1017	three high-fat diets	998:1017	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	3	87	theme	fatty	614:618	arg1	acid					620:623	distinct 18-carbon fatty acid	595:623	distinct 18-carbon fatty acid	595:623	We conducted this study to test whether diets with distinct 18-carbon fatty acid differentially affect inflammatory and metabolic response to LPS exposure in the hypothalamus, liver, and muscle tissues.
34212398	5	88	theme	fatty	1116:1120	arg1	fat					1132:1134	saturated fatty acid-rich fat	1106:1134	saturated fatty acid-rich fat	1106:1134	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	2	89	from	Effects	305:311	arg1	transcription					423:435	neuropeptide gene transcription	405:435	neuropeptide gene transcription	405:435	Effects of 18-carbon fatty acids with various degrees of saturation on inflammatory, oxidative, and neuropeptide gene transcription, especially in the hypothalamus, in response to LPS-induced acute inflammation have not been well studied.
34212398	2	89	from	Effects	305:311	arg1	oxidative					390:398	oxidative	390:398	oxidative	390:398	Effects of 18-carbon fatty acids with various degrees of saturation on inflammatory, oxidative, and neuropeptide gene transcription, especially in the hypothalamus, in response to LPS-induced acute inflammation have not been well studied.
34212398	2	89	from	Effects	305:311	arg1	inflammatory					376:387	inflammatory	376:387	inflammatory	376:387	Effects of 18-carbon fatty acids with various degrees of saturation on inflammatory, oxidative, and neuropeptide gene transcription, especially in the hypothalamus, in response to LPS-induced acute inflammation have not been well studied.
34212398	2	90	theme	LPS-induced	485:495	arg1	inflammation					503:514	LPS-induced acute inflammation	485:514	LPS-induced acute inflammation	485:514	Effects of 18-carbon fatty acids with various degrees of saturation on inflammatory, oxidative, and neuropeptide gene transcription, especially in the hypothalamus, in response to LPS-induced acute inflammation have not been well studied.
34212398	2	91	from	hypothalamus	456:467	arg1	inflammatory					376:387	inflammatory	376:387	inflammatory	376:387	Effects of 18-carbon fatty acids with various degrees of saturation on inflammatory, oxidative, and neuropeptide gene transcription, especially in the hypothalamus, in response to LPS-induced acute inflammation have not been well studied.
34212398	0	92	theme	markers	108:114	arg1	tissue					43:48	peripheral tissue	32:48	peripheral tissue	32:48	Effect of lipopolysaccharide on peripheral tissue and hypothalamic expression of metabolic and inflammatory markers in mice fed high-fat diets with distinct 18-carbon fatty acid composition.
34212398	0	92	theme	markers	108:114	arg1	expression					67:76	hypothalamic expression	54:76	hypothalamic expression	54:76	Effect of lipopolysaccharide on peripheral tissue and hypothalamic expression of metabolic and inflammatory markers in mice fed high-fat diets with distinct 18-carbon fatty acid composition.
34212398	5	93	theme	kcal	939:942	arg1	fat					944:946	5.6% kcal fat	934:946	5.6% kcal fat from lard and 4.4% kcal fat from soybean oil	934:991	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	5	94	theme	25	1036:1037	arg1	%					1038:1038	%	1038:1038	%	1038:1038	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	5	95	contain	containing	1025:1034	arg1	diet					912:915	a control diet	902:915	a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil	902:991	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	5	95	contain	containing	1025:1034	arg1	HFD					1020:1022	HFD	1020:1022	HFD	1020:1022	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	5	95	contain	containing	1025:1034	arg2	fat					1045:1047	25% kcal fat	1036:1047	25% kcal fat	1036:1047	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	5	95	contain	containing	1025:1034	arg1	CON					918:920	CON	918:920	CON	918:920	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	0	96	from	tissue	43:48	arg1	mice					119:122	mice	119:122	mice fed high-fat diets with distinct 18-carbon fatty acid composition	119:188	Effect of lipopolysaccharide on peripheral tissue and hypothalamic expression of metabolic and inflammatory markers in mice fed high-fat diets with distinct 18-carbon fatty acid composition.
34212398	8	97	dep	brain	1547:1551	arg1	the					1543:1545	the	1543:1545	the	1543:1545	LPS challenge increased pro-inflammatory cytokine mRNA expression in the brain and peripheral tissues.
34212398	6	98	theme	higher	1285:1290	arg1	gain					1299:1302	higher weight gain	1285:1302	higher weight gain	1285:1302	Compared to CON, HFD-fed mice had higher weight gain and body fat accumulation.
34212398	5	99	theme	kcal	1040:1043	arg1	fat					1045:1047	25% kcal fat	1036:1047	25% kcal fat	1036:1047	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	10	100	theme	fatty	1715:1719	arg1	acids					1721:1725	18-carbon fatty acids	1705:1725	18-carbon fatty acids	1705:1725	Therefore, saturation degree of 18-carbon fatty acids may not play a critical role in their effects on inflammatory and metabolic indicators in response to acute inflammation induced by LPS.
34212398	5	101	theme	acid-rich	1176:1184	arg1	oil					1186:1188	monounsaturated fatty acid-rich oil	1154:1188	OLO; monounsaturated fatty acid-rich oil	1149:1188	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	3	102	dep	hypothalamus	706:717	arg1	the					702:704	the	702:704	the	702:704	We conducted this study to test whether diets with distinct 18-carbon fatty acid differentially affect inflammatory and metabolic response to LPS exposure in the hypothalamus, liver, and muscle tissues.
34212398	5	103	theme	5.6	934:936	arg1	%					937:937	%	937:937	%	937:937	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	9	104	theme	inflammatory	1649:1660	arg1	responses					1662:1670	LPS-induced inflammatory responses	1637:1670	LPS-induced inflammatory responses	1637:1670	However, the diets did not exert distinguishable effects on LPS-induced inflammatory responses.
34212398	3	105	theme	metabolic	664:672	arg1	response					674:681	inflammatory and metabolic response	647:681	response	674:681	We conducted this study to test whether diets with distinct 18-carbon fatty acid differentially affect inflammatory and metabolic response to LPS exposure in the hypothalamus, liver, and muscle tissues.
34212398	4	106	theme	experimental	752:763	arg1	diets					765:769	Four experimental diets	747:769	Four experimental diets	747:769	Four experimental diets were fed for 4 weeks to male C57BL/6J mice, and a terminal 4-h lipopolysaccharide (LPS) injection was administered.
34212398	2	107	with	acids	332:336	arg1	degrees					351:357	various degrees	343:357	various degrees of saturation	343:371	Effects of 18-carbon fatty acids with various degrees of saturation on inflammatory, oxidative, and neuropeptide gene transcription, especially in the hypothalamus, in response to LPS-induced acute inflammation have not been well studied.
34212398	8	108	theme	LPS	1474:1476	arg1	challenge					1478:1486	LPS challenge	1474:1486	LPS challenge	1474:1486	LPS challenge increased pro-inflammatory cytokine mRNA expression in the brain and peripheral tissues.
34212398	3	109	theme	inflammatory	647:658	arg1	response					674:681	inflammatory and metabolic response	647:681	response	674:681	We conducted this study to test whether diets with distinct 18-carbon fatty acid differentially affect inflammatory and metabolic response to LPS exposure in the hypothalamus, liver, and muscle tissues.
34212398	5	110	theme	acid-rich	1235:1243	arg1	fat					1245:1247	polyunsaturated fatty acid-rich fat	1213:1247	polyunsaturated fatty acid-rich fat	1213:1247	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	2	111	theme	neuropeptide	405:416	arg1	transcription					423:435	neuropeptide gene transcription	405:435	neuropeptide gene transcription	405:435	Effects of 18-carbon fatty acids with various degrees of saturation on inflammatory, oxidative, and neuropeptide gene transcription, especially in the hypothalamus, in response to LPS-induced acute inflammation have not been well studied.
34212398	0	112	theme	hypothalamic	54:65	arg1	expression					67:76	hypothalamic expression	54:76	hypothalamic expression	54:76	Effect of lipopolysaccharide on peripheral tissue and hypothalamic expression of metabolic and inflammatory markers in mice fed high-fat diets with distinct 18-carbon fatty acid composition.
34212398	5	113	theme	%	1065:1065	arg1	fat					1072:1074	20% kcal fat	1063:1074	20% kcal fat	1063:1074	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	5	114	theme	soybean	981:987	arg1	oil					989:991	soybean oil	981:991	soybean oil	981:991	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	2	115	theme	various	343:349	arg1	degrees					351:357	various degrees	343:357	various degrees of saturation	343:371	Effects of 18-carbon fatty acids with various degrees of saturation on inflammatory, oxidative, and neuropeptide gene transcription, especially in the hypothalamus, in response to LPS-induced acute inflammation have not been well studied.
34212398	7	116	theme	lowest	1349:1354	arg1	expression					1362:1371	lowest Cpt1b expression	1349:1371	lowest Cpt1b expression in the liver	1349:1384	The SBO group had lowest Cpt1b expression in the liver, and OLO group had the lowest Pomc and the highest Lepr expression in the hypothalamus.
34212398	7	117	contain	had	1401:1403	arg2	Pomc					1416:1419	Pomc	1416:1419	Pomc	1416:1419	The SBO group had lowest Cpt1b expression in the liver, and OLO group had the lowest Pomc and the highest Lepr expression in the hypothalamus.
34212398	7	117	contain	had	1401:1403	arg2	expression					1442:1451	Lepr expression	1437:1451	the highest Lepr expression in the hypothalamus	1425:1471	The SBO group had lowest Cpt1b expression in the liver, and OLO group had the lowest Pomc and the highest Lepr expression in the hypothalamus.
34212398	7	117	contain	had	1401:1403	arg1	group					1395:1399	OLO group	1391:1399	OLO group	1391:1399	The SBO group had lowest Cpt1b expression in the liver, and OLO group had the lowest Pomc and the highest Lepr expression in the hypothalamus.
34212398	10	118	theme	acids	1721:1725	arg1	degree					1695:1700	saturation degree	1684:1700	saturation degree of 18-carbon fatty acids	1684:1725	Therefore, saturation degree of 18-carbon fatty acids may not play a critical role in their effects on inflammatory and metabolic indicators in response to acute inflammation induced by LPS.
34212398	2	119	theme	acids	332:336	arg1	Effects					305:311	Effects	305:311	Effects of 18-carbon fatty acids with various degrees of saturation on inflammatory, oxidative, and neuropeptide gene transcription, especially in the hypothalamus, in response to LPS-induced acute inflammation	305:514	Effects of 18-carbon fatty acids with various degrees of saturation on inflammatory, oxidative, and neuropeptide gene transcription, especially in the hypothalamus, in response to LPS-induced acute inflammation have not been well studied.
34212398	5	120	theme	acid-rich	1122:1130	arg1	fat					1132:1134	saturated fatty acid-rich fat	1106:1134	saturated fatty acid-rich fat	1106:1134	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	8	121	theme	pro-inflammatory	1498:1513	arg1	expression					1529:1538	pro-inflammatory cytokine mRNA expression	1498:1538	pro-inflammatory cytokine mRNA expression	1498:1538	LPS challenge increased pro-inflammatory cytokine mRNA expression in the brain and peripheral tissues.
34212398	4	122	theme	C57BL/6J	800:807	arg1	mice					809:812	male C57BL/6J mice	795:812	male C57BL/6J mice	795:812	Four experimental diets were fed for 4 weeks to male C57BL/6J mice, and a terminal 4-h lipopolysaccharide (LPS) injection was administered.
34212398	5	123	theme	polyunsaturated	1213:1227	arg1	fat					1245:1247	polyunsaturated fatty acid-rich fat	1213:1247	polyunsaturated fatty acid-rich fat	1213:1247	Diets included a control diet (CON) containing 5.6% kcal fat from lard and 4.4% kcal fat from soybean oil, and three high-fat diets (HFD) containing 25% kcal fat from lard and 20% kcal fat from either shea butter (SHB; saturated fatty acid-rich fat), olive oil (OLO; monounsaturated fatty acid-rich oil), or soybean oil (SBO; polyunsaturated fatty acid-rich fat).
34212398	6	124	theme	body	1308:1311	arg1	accumulation					1317:1328	body fat accumulation	1308:1328	body fat accumulation	1308:1328	Compared to CON, HFD-fed mice had higher weight gain and body fat accumulation.
33246611	11	0	dep	=	1740:1740	arg1	0.98					1751:1754	0.98	1751:1754	0.98	1751:1754	In all 3 species, Brix was highly correlated with IgG and protein concentrations (cows, r = 0.83 and 0.98; goats, r = 0.83 and 0.89; sheep, r = 0.75 and 0.87).
33246611	11	0	dep	=	1740:1740	arg1	0.83					1742:1745	0.83	1742:1745	0.83	1742:1745	In all 3 species, Brix was highly correlated with IgG and protein concentrations (cows, r = 0.83 and 0.98; goats, r = 0.83 and 0.89; sheep, r = 0.75 and 0.87).
33246611	7	1	from	mg/mL	1169:1173	arg1	does					1178:1181	does	1178:1181	does	1178:1181	Thresholds for classification of good colostrum quality (as determined by ELISA) were set at 50 mg IgG/mL in cows and 20 mg/mL in does and ewes.
33246611	7	1	from	mg/mL	1169:1173	arg1	cows					1157:1160	cows	1157:1160	cows	1157:1160	Thresholds for classification of good colostrum quality (as determined by ELISA) were set at 50 mg IgG/mL in cows and 20 mg/mL in does and ewes.
33246611	7	1	from	mg/mL	1169:1173	arg1	ewes					1187:1190	ewes	1187:1190	ewes	1187:1190	Thresholds for classification of good colostrum quality (as determined by ELISA) were set at 50 mg IgG/mL in cows and 20 mg/mL in does and ewes.
33246611	0	2	theme	Short	0:4	arg1	communication					6:18	Short communication	0:18	Short communication: Comparative estimation of colostrum quality by Brix refractometry in bovine, caprine, and ovine colostrum.	0:126	Short communication: Comparative estimation of colostrum quality by Brix refractometry in bovine, caprine, and ovine colostrum.
33246611	13	3	theme	colostrum	2220:2228	arg1	composition					2230:2240	colostrum composition	2220:2240	colostrum composition	2220:2240	In conclusion, Brix refractometry is an acceptable tool for on-farm estimations of colostrum quality in does and ewes despite distinct between-species variations in colostrum composition.
33246611	1	4	theme	timely	160:165	arg1	supply					167:172	the timely supply	156:172	the timely supply of colostrum containing sufficient immunoglobulins to obtain passive immunity against disease	156:266	Newborn ungulates depend on the timely supply of colostrum containing sufficient immunoglobulins to obtain passive immunity against disease.
33246611	12	5	with	Sp	2050:2051	arg1	%					2048:2048	91.3%	2044:2048	91.3%	2044:2048	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	12	5	with	Sp	2050:2051	arg1	%					2035:2035	75%	2033:2035	75% Se	2033:2038	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	12	6	theme	cutoff	1818:1823	arg1	points					1825:1830	Optimal cutoff points	1810:1830	Optimal cutoff points for greatest accuracy of Brix measurements	1810:1873	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	5	7	theme	between-species	649:663	arg1	variations					665:674	between-species variations	649:674	between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values	649:841	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	9	8	theme	other	1364:1368	arg1	species					1370:1376	the other species	1360:1376	the other species	1360:1376	Fat and protein content was higher in sheep colostrum compared with the other species, whereas the highest lactose concentrations were detected in goat colostrum.
33246611	6	9	theme	fat	961:963	arg1	contents					944:951	contents	944:951	contents	944:951	We measured the Brix value and contents of IgG, fat, protein, and lactose in 324 colostrum samples (108 cows, 116 does, and 100 ewes).
33246611	6	9	theme	fat	961:963	arg1	value					934:938	the Brix value	925:938	the Brix value	925:938	We measured the Brix value and contents of IgG, fat, protein, and lactose in 324 colostrum samples (108 cows, 116 does, and 100 ewes).
33246611	12	10	theme	Brix	2014:2017	arg1	%					2012:2012	26.5% Brix	2008:2017	26.5% Brix in ewes (with 75% Se and 91.3% Sp)	2008:2052	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	6	11	dep	116	1023:1025	arg1	does					1027:1030	does	1027:1030	does	1027:1030	We measured the Brix value and contents of IgG, fat, protein, and lactose in 324 colostrum samples (108 cows, 116 does, and 100 ewes).
33246611	2	12	theme	on-farm	304:310	arg1	estimation					312:321	a rapid on-farm estimation	296:321	a rapid on-farm estimation of colostrum quality	296:342	Brix refractometry enables a rapid on-farm estimation of colostrum quality and has been intensively studied in bovines.
33246611	12	13	from	%	1884:1884	arg1	ewes					2022:2025	ewes	2022:2025	ewes (with 75% Se and 91.3% Sp)	2022:2052	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	12	13	from	%	1884:1884	arg1	Sp					1999:2000	Sp	1999:2000	Sp	1999:2000	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	12	13	from	%	1884:1884	arg1	Sp					2050:2051	Sp	2050:2051	Sp	2050:2051	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	12	13	from	%	1884:1884	arg1	does					1970:1973	does	1970:1973	does	1970:1973	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	12	13	from	%	1884:1884	arg1	cows					1894:1897	cows	1894:1897	cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)]	1894:1953	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	6	14	theme	IgG	956:958	arg1	contents					944:951	contents	944:951	contents	944:951	We measured the Brix value and contents of IgG, fat, protein, and lactose in 324 colostrum samples (108 cows, 116 does, and 100 ewes).
33246611	6	14	theme	IgG	956:958	arg1	value					934:938	the Brix value	925:938	the Brix value	925:938	We measured the Brix value and contents of IgG, fat, protein, and lactose in 324 colostrum samples (108 cows, 116 does, and 100 ewes).
33246611	11	15	theme	=	1766:1766	arg1	r					1764:1764	r	1764:1764	r = 0.83 and 0.89	1764:1780	In all 3 species, Brix was highly correlated with IgG and protein concentrations (cows, r = 0.83 and 0.98; goats, r = 0.83 and 0.89; sheep, r = 0.75 and 0.87).
33246611	0	16	from	refractometry	73:85	arg1	colostrum					117:125	ovine colostrum	111:125	ovine colostrum	111:125	Short communication: Comparative estimation of colostrum quality by Brix refractometry in bovine, caprine, and ovine colostrum.
33246611	0	16	from	refractometry	73:85	arg1	caprine					98:104	caprine	98:104	caprine	98:104	Short communication: Comparative estimation of colostrum quality by Brix refractometry in bovine, caprine, and ovine colostrum.
33246611	0	16	from	refractometry	73:85	arg1	bovine					90:95	bovine	90:95	bovine	90:95	Short communication: Comparative estimation of colostrum quality by Brix refractometry in bovine, caprine, and ovine colostrum.
33246611	8	17	theme	ovine	1275:1279	arg1	colostrum					1281:1289	ovine colostrum	1275:1289	ovine colostrum	1275:1289	Bovine colostrum showed the greatest IgG concentrations compared with caprine and ovine colostrum.
33246611	8	18	theme	Bovine	1193:1198	arg1	colostrum					1200:1208	Bovine colostrum	1193:1208	Bovine colostrum	1193:1208	Bovine colostrum showed the greatest IgG concentrations compared with caprine and ovine colostrum.
33246611	5	19	theme	Brix	705:708	arg1	values					710:715	Brix values	705:715	Brix values	705:715	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	5	19	theme	Brix	705:708	arg1	protein					755:761	protein	755:761	protein	755:761	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	5	19	theme	Brix	705:708	arg1	lactose					768:774	lactose	768:774	lactose	768:774	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	5	19	theme	Brix	705:708	arg1	fat					750:752	fat	750:752	fat	750:752	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	5	19	theme	Brix	705:708	arg1	IgG					745:747	IgG	745:747	IgG	745:747	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	9	20	theme	lactose	1399:1405	arg1	concentrations					1407:1420	the highest lactose concentrations	1387:1420	the highest lactose concentrations	1387:1420	Fat and protein content was higher in sheep colostrum compared with the other species, whereas the highest lactose concentrations were detected in goat colostrum.
33246611	12	21	dep	%	1985:1985	arg1	Se					1987:1988	Se	1987:1988	53.5% Se	1981:1988	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	6	22	theme	colostrum	994:1002	arg1	samples					1004:1010	324 colostrum samples	990:1010	324 colostrum samples (108 cows, 116 does, and 100 ewes)	990:1045	We measured the Brix value and contents of IgG, fat, protein, and lactose in 324 colostrum samples (108 cows, 116 does, and 100 ewes).
33246611	13	23	from	variations	2206:2215	arg1	composition					2230:2240	colostrum composition	2220:2240	colostrum composition	2220:2240	In conclusion, Brix refractometry is an acceptable tool for on-farm estimations of colostrum quality in does and ewes despite distinct between-species variations in colostrum composition.
33246611	8	24	theme	greatest	1221:1228	arg1	concentrations					1234:1247	the greatest IgG concentrations	1217:1247	the greatest IgG concentrations	1217:1247	Bovine colostrum showed the greatest IgG concentrations compared with caprine and ovine colostrum.
33246611	10	25	theme	bovine	1598:1603	arg1	ovine					1606:1610	bovine, ovine, and caprine colostrum	1598:1633	ovine	1606:1610	Brix values ranged from 11.4 to 34.6% (22.1 ± 4.2%; mean ± standard deviation), 15.4 to 40.0% (28.5 ± 6.8%), and 8.8 to 39.8% (21.6 ± 5.3%) in bovine, ovine, and caprine colostrum, respectively.
33246611	5	26	theme	curve	897:901	arg1	analysis					903:910	characteristic curve analysis	882:910	characteristic curve analysis	882:910	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	12	27	from	%	1960:1960	arg1	ewes					2022:2025	ewes	2022:2025	ewes (with 75% Se and 91.3% Sp)	2022:2052	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	12	27	from	%	1960:1960	arg1	Sp					1999:2000	Sp	1999:2000	Sp	1999:2000	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	12	27	from	%	1960:1960	arg1	Sp					2050:2051	Sp	2050:2051	Sp	2050:2051	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	12	27	from	%	1960:1960	arg1	does					1970:1973	does	1970:1973	does	1970:1973	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	12	27	from	%	1960:1960	arg1	cows					1894:1897	cows	1894:1897	cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)]	1894:1953	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	7	28	theme	mg	1144:1145	arg1	IgG/mL					1147:1152	50 mg IgG/mL	1141:1152	50 mg IgG/mL in cows	1141:1160	Thresholds for classification of good colostrum quality (as determined by ELISA) were set at 50 mg IgG/mL in cows and 20 mg/mL in does and ewes.
33246611	13	29	theme	between-species	2190:2204	arg1	variations					2206:2215	distinct between-species variations	2181:2215	distinct between-species variations in colostrum composition	2181:2240	In conclusion, Brix refractometry is an acceptable tool for on-farm estimations of colostrum quality in does and ewes despite distinct between-species variations in colostrum composition.
33246611	12	30	dep	cows	1894:1897	arg1	[with					1899:1903	[with	1899:1903	[with	1899:1903	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	5	31	dep	values	710:715	arg1	values					710:715	Brix values	705:715	Brix values	705:715	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	5	31	dep	values	710:715	arg1	protein					755:761	protein	755:761	protein	755:761	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	5	31	dep	values	710:715	arg1	lactose					768:774	lactose	768:774	lactose	768:774	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	5	31	dep	values	710:715	arg1	fat					750:752	fat	750:752	fat	750:752	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	5	31	dep	values	710:715	arg1	IgG					745:747	IgG	745:747	IgG	745:747	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	4	32	theme	present	520:526	arg1	study					528:532	The present study	516:532	The present study	516:532	The present study compared bovine, caprine, and ovine colostrum quality estimation using an optical Brix refractometer.
33246611	6	33	theme	protein	966:972	arg1	contents					944:951	contents	944:951	contents	944:951	We measured the Brix value and contents of IgG, fat, protein, and lactose in 324 colostrum samples (108 cows, 116 does, and 100 ewes).
33246611	6	33	theme	protein	966:972	arg1	value					934:938	the Brix value	925:938	the Brix value	925:938	We measured the Brix value and contents of IgG, fat, protein, and lactose in 324 colostrum samples (108 cows, 116 does, and 100 ewes).
33246611	12	34	theme	%	1909:1909	arg1	Se					1924:1925	Se	1924:1925	Se	1924:1925	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	12	34	theme	%	1909:1909	arg1	sensitivity					1911:1921	87.1% sensitivity	1905:1921	87.1% sensitivity (Se)	1905:1926	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	3	35	theme	colostrum	451:459	arg1	quality					461:467	colostrum quality	451:467	colostrum quality in goats and ewes	451:485	However, the suitability of Brix refractometers for assessing colostrum quality in goats and ewes has been scarcely evaluated.
33246611	13	36	theme	colostrum	2138:2146	arg1	quality					2148:2154	colostrum quality	2138:2154	colostrum quality	2138:2154	In conclusion, Brix refractometry is an acceptable tool for on-farm estimations of colostrum quality in does and ewes despite distinct between-species variations in colostrum composition.
33246611	7	37	dep	quality	1096:1102	arg1	determined					1108:1117	determined	1108:1117	determined by ELISA	1108:1126	Thresholds for classification of good colostrum quality (as determined by ELISA) were set at 50 mg IgG/mL in cows and 20 mg/mL in does and ewes.
33246611	13	38	theme	quality	2148:2154	arg1	estimations					2123:2133	on-farm estimations	2115:2133	on-farm estimations of colostrum quality	2115:2154	In conclusion, Brix refractometry is an acceptable tool for on-farm estimations of colostrum quality in does and ewes despite distinct between-species variations in colostrum composition.
33246611	10	39	theme	standard	1514:1521	arg1	deviation					1523:1531	mean ± standard deviation	1507:1531	mean ± standard deviation	1507:1531	Brix values ranged from 11.4 to 34.6% (22.1 ± 4.2%; mean ± standard deviation), 15.4 to 40.0% (28.5 ± 6.8%), and 8.8 to 39.8% (21.6 ± 5.3%) in bovine, ovine, and caprine colostrum, respectively.
33246611	5	40	theme	refractometry	802:814	arg1	accuracy					785:792	the accuracy	781:792	the accuracy of Brix refractometry at different cutoff values	781:841	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	5	40	theme	refractometry	802:814	arg1	constituents					731:742	colostrum constituents	721:742	colostrum constituents	721:742	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	5	40	theme	refractometry	802:814	arg1	IgG					745:747	IgG	745:747	IgG	745:747	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	5	40	theme	refractometry	802:814	arg1	lactose					768:774	lactose	768:774	lactose	768:774	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	5	40	theme	refractometry	802:814	arg1	values					710:715	Brix values	705:715	Brix values	705:715	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	5	40	theme	refractometry	802:814	arg1	fat					750:752	fat	750:752	fat	750:752	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	5	40	theme	refractometry	802:814	arg1	protein					755:761	protein	755:761	protein	755:761	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	5	41	theme	different	819:827	arg1	values					836:841	different cutoff values	819:841	different cutoff values	819:841	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	1	42	theme	Newborn	128:134	arg1	ungulates					136:144	Newborn ungulates	128:144	Newborn ungulates	128:144	Newborn ungulates depend on the timely supply of colostrum containing sufficient immunoglobulins to obtain passive immunity against disease.
33246611	3	43	theme	Brix	417:420	arg1	refractometers					422:435	Brix refractometers	417:435	Brix refractometers	417:435	However, the suitability of Brix refractometers for assessing colostrum quality in goats and ewes has been scarcely evaluated.
33246611	4	44	theme	ovine	564:568	arg1	estimation					588:597	bovine, caprine, and ovine colostrum quality estimation	543:597	estimation	588:597	The present study compared bovine, caprine, and ovine colostrum quality estimation using an optical Brix refractometer.
33246611	12	45	theme	Brix	1857:1860	arg1	measurements					1862:1873	Brix measurements	1857:1873	Brix measurements	1857:1873	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	13	46	theme	Brix	2070:2073	arg1	refractometry					2075:2087	Brix refractometry	2070:2087	Brix refractometry	2070:2087	In conclusion, Brix refractometry is an acceptable tool for on-farm estimations of colostrum quality in does and ewes despite distinct between-species variations in colostrum composition.
33246611	13	46	theme	Brix	2070:2073	arg1	tool					2106:2109	an acceptable tool	2092:2109	an acceptable tool for on-farm estimations of colostrum quality in does and ewes	2092:2171	In conclusion, Brix refractometry is an acceptable tool for on-farm estimations of colostrum quality in does and ewes despite distinct between-species variations in colostrum composition.
33246611	10	47	theme	±	1555:1555	arg1	%					1560:1560	28.5 ± 6.8%	1550:1560	28.5 ± 6.8%	1550:1560	Brix values ranged from 11.4 to 34.6% (22.1 ± 4.2%; mean ± standard deviation), 15.4 to 40.0% (28.5 ± 6.8%), and 8.8 to 39.8% (21.6 ± 5.3%) in bovine, ovine, and caprine colostrum, respectively.
33246611	10	47	theme	±	1555:1555	arg1	%					1547:1547	15.4 to 40.0%	1535:1547	15.4 to 40.0% (28.5 ± 6.8%)	1535:1561	Brix values ranged from 11.4 to 34.6% (22.1 ± 4.2%; mean ± standard deviation), 15.4 to 40.0% (28.5 ± 6.8%), and 8.8 to 39.8% (21.6 ± 5.3%) in bovine, ovine, and caprine colostrum, respectively.
33246611	7	48	theme	colostrum	1086:1094	arg1	quality					1096:1102	good colostrum quality (as determined by ELISA)	1081:1127	good colostrum quality (as determined by ELISA)	1081:1127	Thresholds for classification of good colostrum quality (as determined by ELISA) were set at 50 mg IgG/mL in cows and 20 mg/mL in does and ewes.
33246611	5	49	from	variations	665:674	arg1	relationships					683:695	the relationships	679:695	the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values	679:841	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	4	50	theme	optical	608:614	arg1	refractometer					621:633	an optical Brix refractometer	605:633	an optical Brix refractometer	605:633	The present study compared bovine, caprine, and ovine colostrum quality estimation using an optical Brix refractometer.
33246611	11	51	dep	=	1766:1766	arg1	0.89					1777:1780	0.89	1777:1780	0.89	1777:1780	In all 3 species, Brix was highly correlated with IgG and protein concentrations (cows, r = 0.83 and 0.98; goats, r = 0.83 and 0.89; sheep, r = 0.75 and 0.87).
33246611	11	51	dep	=	1766:1766	arg1	0.83					1768:1771	0.83	1768:1771	0.83	1768:1771	In all 3 species, Brix was highly correlated with IgG and protein concentrations (cows, r = 0.83 and 0.98; goats, r = 0.83 and 0.89; sheep, r = 0.75 and 0.87).
33246611	1	52	theme	passive	235:241	arg1	immunity					243:250	passive immunity	235:250	passive immunity against disease	235:266	Newborn ungulates depend on the timely supply of colostrum containing sufficient immunoglobulins to obtain passive immunity against disease.
33246611	7	53	theme	good	1081:1084	arg1	quality					1096:1102	good colostrum quality (as determined by ELISA)	1081:1127	good colostrum quality (as determined by ELISA)	1081:1127	Thresholds for classification of good colostrum quality (as determined by ELISA) were set at 50 mg IgG/mL in cows and 20 mg/mL in does and ewes.
33246611	6	54	theme	Brix	929:932	arg1	value					934:938	the Brix value	925:938	the Brix value	925:938	We measured the Brix value and contents of IgG, fat, protein, and lactose in 324 colostrum samples (108 cows, 116 does, and 100 ewes).
33246611	11	55	dep	=	1792:1792	arg1	cows					1732:1735	cows	1732:1735	cows	1732:1735	In all 3 species, Brix was highly correlated with IgG and protein concentrations (cows, r = 0.83 and 0.98; goats, r = 0.83 and 0.89; sheep, r = 0.75 and 0.87).
33246611	11	55	dep	=	1792:1792	arg1	r					1738:1738	r	1738:1738	r = 0.83 and 0.98	1738:1754	In all 3 species, Brix was highly correlated with IgG and protein concentrations (cows, r = 0.83 and 0.98; goats, r = 0.83 and 0.89; sheep, r = 0.75 and 0.87).
33246611	11	55	dep	=	1792:1792	arg1	goats					1757:1761	goats	1757:1761	goats	1757:1761	In all 3 species, Brix was highly correlated with IgG and protein concentrations (cows, r = 0.83 and 0.98; goats, r = 0.83 and 0.89; sheep, r = 0.75 and 0.87).
33246611	11	55	dep	=	1792:1792	arg1	r					1764:1764	r	1764:1764	r = 0.83 and 0.89	1764:1780	In all 3 species, Brix was highly correlated with IgG and protein concentrations (cows, r = 0.83 and 0.98; goats, r = 0.83 and 0.89; sheep, r = 0.75 and 0.87).
33246611	11	55	dep	=	1792:1792	arg1	sheep					1783:1787	sheep	1783:1787	sheep	1783:1787	In all 3 species, Brix was highly correlated with IgG and protein concentrations (cows, r = 0.83 and 0.98; goats, r = 0.83 and 0.89; sheep, r = 0.75 and 0.87).
33246611	10	56	theme	Brix	1455:1458	arg1	values					1460:1465	Brix values	1455:1465	Brix values	1455:1465	Brix values ranged from 11.4 to 34.6% (22.1 ± 4.2%; mean ± standard deviation), 15.4 to 40.0% (28.5 ± 6.8%), and 8.8 to 39.8% (21.6 ± 5.3%) in bovine, ovine, and caprine colostrum, respectively.
33246611	10	57	dep	%	1504:1504	arg1	deviation					1523:1531	mean ± standard deviation	1507:1531	mean ± standard deviation	1507:1531	Brix values ranged from 11.4 to 34.6% (22.1 ± 4.2%; mean ± standard deviation), 15.4 to 40.0% (28.5 ± 6.8%), and 8.8 to 39.8% (21.6 ± 5.3%) in bovine, ovine, and caprine colostrum, respectively.
33246611	3	58	from	quality	461:467	arg1	goats					472:476	goats	472:476	goats	472:476	However, the suitability of Brix refractometers for assessing colostrum quality in goats and ewes has been scarcely evaluated.
33246611	3	58	from	quality	461:467	arg1	ewes					482:485	ewes	482:485	ewes	482:485	However, the suitability of Brix refractometers for assessing colostrum quality in goats and ewes has been scarcely evaluated.
33246611	4	59	theme	quality	580:586	arg1	estimation					588:597	bovine, caprine, and ovine colostrum quality estimation	543:597	estimation	588:597	The present study compared bovine, caprine, and ovine colostrum quality estimation using an optical Brix refractometer.
33246611	2	60	theme	rapid	298:302	arg1	estimation					312:321	a rapid on-farm estimation	296:321	a rapid on-farm estimation of colostrum quality	296:342	Brix refractometry enables a rapid on-farm estimation of colostrum quality and has been intensively studied in bovines.
33246611	9	61	theme	sheep	1330:1334	arg1	colostrum					1336:1344	sheep colostrum	1330:1344	sheep colostrum	1330:1344	Fat and protein content was higher in sheep colostrum compared with the other species, whereas the highest lactose concentrations were detected in goat colostrum.
33246611	2	62	theme	colostrum	326:334	arg1	quality					336:342	colostrum quality	326:342	colostrum quality	326:342	Brix refractometry enables a rapid on-farm estimation of colostrum quality and has been intensively studied in bovines.
33246611	13	63	from	tool	2106:2109	arg1	ewes					2168:2171	ewes	2168:2171	ewes	2168:2171	In conclusion, Brix refractometry is an acceptable tool for on-farm estimations of colostrum quality in does and ewes despite distinct between-species variations in colostrum composition.
33246611	13	63	from	tool	2106:2109	arg1	does					2159:2162	does	2159:2162	does	2159:2162	In conclusion, Brix refractometry is an acceptable tool for on-farm estimations of colostrum quality in does and ewes despite distinct between-species variations in colostrum composition.
33246611	13	63	from	tool	2106:2109	arg1	conclusion					2058:2067	conclusion	2058:2067	conclusion	2058:2067	In conclusion, Brix refractometry is an acceptable tool for on-farm estimations of colostrum quality in does and ewes despite distinct between-species variations in colostrum composition.
33246611	11	64	theme	=	1740:1740	arg1	r					1738:1738	r	1738:1738	r = 0.83 and 0.98	1738:1754	In all 3 species, Brix was highly correlated with IgG and protein concentrations (cows, r = 0.83 and 0.98; goats, r = 0.83 and 0.89; sheep, r = 0.75 and 0.87).
33246611	0	65	theme	colostrum	47:55	arg1	quality					57:63	colostrum quality	47:63	colostrum quality	47:63	Short communication: Comparative estimation of colostrum quality by Brix refractometry in bovine, caprine, and ovine colostrum.
33246611	10	66	theme	±	1499:1499	arg1	%					1504:1504	22.1 ± 4.2%	1494:1504	22.1 ± 4.2%	1494:1504	Brix values ranged from 11.4 to 34.6% (22.1 ± 4.2%; mean ± standard deviation), 15.4 to 40.0% (28.5 ± 6.8%), and 8.8 to 39.8% (21.6 ± 5.3%) in bovine, ovine, and caprine colostrum, respectively.
33246611	10	66	theme	±	1499:1499	arg1	%					1491:1491	11.4 to 34.6%	1479:1491	11.4 to 34.6% (22.1 ± 4.2%; mean ± standard deviation)	1479:1532	Brix values ranged from 11.4 to 34.6% (22.1 ± 4.2%; mean ± standard deviation), 15.4 to 40.0% (28.5 ± 6.8%), and 8.8 to 39.8% (21.6 ± 5.3%) in bovine, ovine, and caprine colostrum, respectively.
33246611	10	67	theme	±	1587:1587	arg1	%					1592:1592	21.6 ± 5.3%	1582:1592	21.6 ± 5.3%	1582:1592	Brix values ranged from 11.4 to 34.6% (22.1 ± 4.2%; mean ± standard deviation), 15.4 to 40.0% (28.5 ± 6.8%), and 8.8 to 39.8% (21.6 ± 5.3%) in bovine, ovine, and caprine colostrum, respectively.
33246611	10	67	theme	±	1587:1587	arg1	%					1579:1579	8.8 to 39.8%	1568:1579	8.8 to 39.8% (21.6 ± 5.3%)	1568:1593	Brix values ranged from 11.4 to 34.6% (22.1 ± 4.2%; mean ± standard deviation), 15.4 to 40.0% (28.5 ± 6.8%), and 8.8 to 39.8% (21.6 ± 5.3%) in bovine, ovine, and caprine colostrum, respectively.
33246611	12	68	theme	Optimal	1810:1816	arg1	points					1825:1830	Optimal cutoff points	1810:1830	Optimal cutoff points for greatest accuracy of Brix measurements	1810:1873	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	0	69	theme	ovine	111:115	arg1	colostrum					117:125	ovine colostrum	111:125	ovine colostrum	111:125	Short communication: Comparative estimation of colostrum quality by Brix refractometry in bovine, caprine, and ovine colostrum.
33246611	1	70	theme	colostrum	177:185	arg1	supply					167:172	the timely supply	156:172	the timely supply of colostrum containing sufficient immunoglobulins to obtain passive immunity against disease	156:266	Newborn ungulates depend on the timely supply of colostrum containing sufficient immunoglobulins to obtain passive immunity against disease.
33246611	10	71	theme	mean	1507:1510	arg1	deviation					1523:1531	mean ± standard deviation	1507:1531	mean ± standard deviation	1507:1531	Brix values ranged from 11.4 to 34.6% (22.1 ± 4.2%; mean ± standard deviation), 15.4 to 40.0% (28.5 ± 6.8%), and 8.8 to 39.8% (21.6 ± 5.3%) in bovine, ovine, and caprine colostrum, respectively.
33246611	10	72	dep	34.6	1487:1490	arg1	to					1484:1485	to	1484:1485	to	1484:1485	Brix values ranged from 11.4 to 34.6% (22.1 ± 4.2%; mean ± standard deviation), 15.4 to 40.0% (28.5 ± 6.8%), and 8.8 to 39.8% (21.6 ± 5.3%) in bovine, ovine, and caprine colostrum, respectively.
33246611	13	73	theme	acceptable	2095:2104	arg1	refractometry					2075:2087	Brix refractometry	2070:2087	Brix refractometry	2070:2087	In conclusion, Brix refractometry is an acceptable tool for on-farm estimations of colostrum quality in does and ewes despite distinct between-species variations in colostrum composition.
33246611	13	73	theme	acceptable	2095:2104	arg1	tool					2106:2109	an acceptable tool	2092:2109	an acceptable tool for on-farm estimations of colostrum quality in does and ewes	2092:2171	In conclusion, Brix refractometry is an acceptable tool for on-farm estimations of colostrum quality in does and ewes despite distinct between-species variations in colostrum composition.
33246611	11	74	theme	protein	1708:1714	arg1	concentrations					1716:1729	protein concentrations	1708:1729	protein concentrations	1708:1729	In all 3 species, Brix was highly correlated with IgG and protein concentrations (cows, r = 0.83 and 0.98; goats, r = 0.83 and 0.89; sheep, r = 0.75 and 0.87).
33246611	12	75	from	%	2012:2012	arg1	ewes					2022:2025	ewes	2022:2025	ewes (with 75% Se and 91.3% Sp)	2022:2052	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	12	75	from	%	2012:2012	arg1	Sp					1999:2000	Sp	1999:2000	Sp	1999:2000	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	12	75	from	%	2012:2012	arg1	Sp					2050:2051	Sp	2050:2051	Sp	2050:2051	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	12	75	from	%	2012:2012	arg1	does					1970:1973	does	1970:1973	does	1970:1973	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	12	75	from	%	2012:2012	arg1	cows					1894:1897	cows	1894:1897	cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)]	1894:1953	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	1	76	theme	sufficient	198:207	arg1	immunoglobulins					209:223	sufficient immunoglobulins	198:223	sufficient immunoglobulins	198:223	Newborn ungulates depend on the timely supply of colostrum containing sufficient immunoglobulins to obtain passive immunity against disease.
33246611	12	77	theme	Brix	1962:1965	arg1	%					1960:1960	20.7% Brix	1956:1965	20.7% Brix in does (with 53.5% Se and 100% Sp)	1956:2001	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	9	78	theme	highest	1391:1397	arg1	concentrations					1407:1420	the highest lactose concentrations	1387:1420	the highest lactose concentrations	1387:1420	Fat and protein content was higher in sheep colostrum compared with the other species, whereas the highest lactose concentrations were detected in goat colostrum.
33246611	12	79	theme	%	1935:1935	arg1	Sp					1950:1951	Sp	1950:1951	Sp	1950:1951	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	12	79	theme	%	1935:1935	arg1	specificity					1937:1947	100% specificity	1932:1947	100% specificity (Sp)	1932:1952	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	9	80	located	detected	1427:1434	arg2	concentrations					1407:1420	the highest lactose concentrations	1387:1420	the highest lactose concentrations	1387:1420	Fat and protein content was higher in sheep colostrum compared with the other species, whereas the highest lactose concentrations were detected in goat colostrum.
33246611	9	80	located	detected	1427:1434	arg1	colostrum					1444:1452	goat colostrum	1439:1452	goat colostrum	1439:1452	Fat and protein content was higher in sheep colostrum compared with the other species, whereas the highest lactose concentrations were detected in goat colostrum.
33246611	10	81	dep	%	1579:1579	arg1	to					1572:1573	to	1572:1573	to	1572:1573	Brix values ranged from 11.4 to 34.6% (22.1 ± 4.2%; mean ± standard deviation), 15.4 to 40.0% (28.5 ± 6.8%), and 8.8 to 39.8% (21.6 ± 5.3%) in bovine, ovine, and caprine colostrum, respectively.
33246611	2	82	theme	Brix	269:272	arg1	refractometry					274:286	Brix refractometry	269:286	Brix refractometry	269:286	Brix refractometry enables a rapid on-farm estimation of colostrum quality and has been intensively studied in bovines.
33246611	5	83	from	values	836:841	arg1	accuracy					785:792	the accuracy	781:792	the accuracy of Brix refractometry at different cutoff values	781:841	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	5	83	from	values	836:841	arg1	constituents					731:742	colostrum constituents	721:742	colostrum constituents	721:742	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	5	83	from	values	836:841	arg1	IgG					745:747	IgG	745:747	IgG	745:747	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	5	83	from	values	836:841	arg1	lactose					768:774	lactose	768:774	lactose	768:774	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	5	83	from	values	836:841	arg1	values					710:715	Brix values	705:715	Brix values	705:715	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	5	83	from	values	836:841	arg1	fat					750:752	fat	750:752	fat	750:752	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	5	83	from	values	836:841	arg1	protein					755:761	protein	755:761	protein	755:761	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	7	84	from	IgG/mL	1147:1152	arg1	does					1178:1181	does	1178:1181	does	1178:1181	Thresholds for classification of good colostrum quality (as determined by ELISA) were set at 50 mg IgG/mL in cows and 20 mg/mL in does and ewes.
33246611	7	84	from	IgG/mL	1147:1152	arg1	cows					1157:1160	cows	1157:1160	cows	1157:1160	Thresholds for classification of good colostrum quality (as determined by ELISA) were set at 50 mg IgG/mL in cows and 20 mg/mL in does and ewes.
33246611	7	84	from	IgG/mL	1147:1152	arg1	ewes					1187:1190	ewes	1187:1190	ewes	1187:1190	Thresholds for classification of good colostrum quality (as determined by ELISA) were set at 50 mg IgG/mL in cows and 20 mg/mL in does and ewes.
33246611	0	85	theme	Brix	68:71	arg1	refractometry					73:85	Brix refractometry	68:85	Brix refractometry in bovine, caprine, and ovine colostrum	68:125	Short communication: Comparative estimation of colostrum quality by Brix refractometry in bovine, caprine, and ovine colostrum.
33246611	5	86	theme	cutoff	829:834	arg1	values					836:841	different cutoff values	819:841	different cutoff values	819:841	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	5	87	theme	characteristic	882:895	arg1	analysis					903:910	characteristic curve analysis	882:910	characteristic curve analysis	882:910	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	6	88	theme	324	990:992	arg1	samples					1004:1010	324 colostrum samples	990:1010	324 colostrum samples (108 cows, 116 does, and 100 ewes)	990:1045	We measured the Brix value and contents of IgG, fat, protein, and lactose in 324 colostrum samples (108 cows, 116 does, and 100 ewes).
33246611	12	89	theme	100	1932:1934	arg1	%					1935:1935	%	1935:1935	%	1935:1935	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	5	90	theme	colostrum	721:729	arg1	constituents					731:742	colostrum constituents	721:742	colostrum constituents	721:742	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	7	91	theme	50	1141:1142	arg1	mg					1144:1145	mg	1144:1145	mg	1144:1145	Thresholds for classification of good colostrum quality (as determined by ELISA) were set at 50 mg IgG/mL in cows and 20 mg/mL in does and ewes.
33246611	9	92	theme	goat	1439:1442	arg1	colostrum					1444:1452	goat colostrum	1439:1452	goat colostrum	1439:1452	Fat and protein content was higher in sheep colostrum compared with the other species, whereas the highest lactose concentrations were detected in goat colostrum.
33246611	6	93	theme	lactose	979:985	arg1	contents					944:951	contents	944:951	contents	944:951	We measured the Brix value and contents of IgG, fat, protein, and lactose in 324 colostrum samples (108 cows, 116 does, and 100 ewes).
33246611	6	93	theme	lactose	979:985	arg1	value					934:938	the Brix value	925:938	the Brix value	925:938	We measured the Brix value and contents of IgG, fat, protein, and lactose in 324 colostrum samples (108 cows, 116 does, and 100 ewes).
33246611	7	94	dep	determined	1108:1117	arg1	as					1105:1106	as	1105:1106	as	1105:1106	Thresholds for classification of good colostrum quality (as determined by ELISA) were set at 50 mg IgG/mL in cows and 20 mg/mL in does and ewes.
33246611	8	95	theme	IgG	1230:1232	arg1	concentrations					1234:1247	the greatest IgG concentrations	1217:1247	the greatest IgG concentrations	1217:1247	Bovine colostrum showed the greatest IgG concentrations compared with caprine and ovine colostrum.
33246611	13	96	theme	on-farm	2115:2121	arg1	estimations					2123:2133	on-farm estimations	2115:2133	on-farm estimations of colostrum quality	2115:2154	In conclusion, Brix refractometry is an acceptable tool for on-farm estimations of colostrum quality in does and ewes despite distinct between-species variations in colostrum composition.
33246611	13	97	theme	distinct	2181:2188	arg1	variations					2206:2215	distinct between-species variations	2181:2215	distinct between-species variations in colostrum composition	2181:2240	In conclusion, Brix refractometry is an acceptable tool for on-farm estimations of colostrum quality in does and ewes despite distinct between-species variations in colostrum composition.
33246611	6	98	dep	samples	1004:1010	arg1	cows					1017:1020	108 cows, 116 does,	1013:1031	cows	1017:1020	We measured the Brix value and contents of IgG, fat, protein, and lactose in 324 colostrum samples (108 cows, 116 does, and 100 ewes).
33246611	6	98	dep	samples	1004:1010	arg1	ewes					1041:1044	100 ewes	1037:1044	100 ewes	1037:1044	We measured the Brix value and contents of IgG, fat, protein, and lactose in 324 colostrum samples (108 cows, 116 does, and 100 ewes).
33246611	12	99	with	Sp	1999:2000	arg1	%					1985:1985	53.5%	1981:1985	53.5% Se	1981:1988	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	12	99	with	Sp	1999:2000	arg1	%					1997:1997	100%	1994:1997	100%	1994:1997	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	6	100	dep	cows	1017:1020	arg1	116					1023:1025	116	1023:1025	116	1023:1025	We measured the Brix value and contents of IgG, fat, protein, and lactose in 324 colostrum samples (108 cows, 116 does, and 100 ewes).
33246611	5	101	theme	Brix	797:800	arg1	refractometry					802:814	Brix refractometry	797:814	Brix refractometry	797:814	In addition, between-species variations in the relationships between Brix values and colostrum constituents (IgG, fat, protein, and lactose) and the accuracy of Brix refractometry at different cutoff values were evaluated by a receiver operating characteristic curve analysis.
33246611	10	102	dep	%	1547:1547	arg1	to					1540:1541	to	1540:1541	to	1540:1541	Brix values ranged from 11.4 to 34.6% (22.1 ± 4.2%; mean ± standard deviation), 15.4 to 40.0% (28.5 ± 6.8%), and 8.8 to 39.8% (21.6 ± 5.3%) in bovine, ovine, and caprine colostrum, respectively.
33246611	10	103	theme	±	1512:1512	arg1	deviation					1523:1531	mean ± standard deviation	1507:1531	mean ± standard deviation	1507:1531	Brix values ranged from 11.4 to 34.6% (22.1 ± 4.2%; mean ± standard deviation), 15.4 to 40.0% (28.5 ± 6.8%), and 8.8 to 39.8% (21.6 ± 5.3%) in bovine, ovine, and caprine colostrum, respectively.
33246611	0	104	theme	Comparative	21:31	arg1	estimation					33:42	Comparative estimation	21:42	Short communication: Comparative estimation of colostrum quality by Brix refractometry in bovine, caprine, and ovine colostrum.	0:126	Short communication: Comparative estimation of colostrum quality by Brix refractometry in bovine, caprine, and ovine colostrum.
33246611	4	105	theme	colostrum	570:578	arg1	estimation					588:597	bovine, caprine, and ovine colostrum quality estimation	543:597	estimation	588:597	The present study compared bovine, caprine, and ovine colostrum quality estimation using an optical Brix refractometer.
33246611	12	106	theme	Brix	1886:1889	arg1	%					1884:1884	19.3% Brix	1880:1889	19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)]	1880:1953	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	7	107	theme	quality	1096:1102	arg1	classification					1063:1076	classification	1063:1076	classification of good colostrum quality (as determined by ELISA)	1063:1127	Thresholds for classification of good colostrum quality (as determined by ELISA) were set at 50 mg IgG/mL in cows and 20 mg/mL in does and ewes.
33246611	3	108	theme	refractometers	422:435	arg1	suitability					402:412	the suitability	398:412	the suitability of Brix refractometers for assessing colostrum quality in goats and ewes	398:485	However, the suitability of Brix refractometers for assessing colostrum quality in goats and ewes has been scarcely evaluated.
33246611	4	109	theme	bovine	543:548	arg1	caprine					551:557	bovine, caprine, and ovine colostrum quality estimation	543:597	caprine	551:557	The present study compared bovine, caprine, and ovine colostrum quality estimation using an optical Brix refractometer.
33246611	10	110	theme	caprine	1617:1623	arg1	colostrum					1625:1633	bovine, ovine, and caprine colostrum	1598:1633	colostrum	1625:1633	Brix values ranged from 11.4 to 34.6% (22.1 ± 4.2%; mean ± standard deviation), 15.4 to 40.0% (28.5 ± 6.8%), and 8.8 to 39.8% (21.6 ± 5.3%) in bovine, ovine, and caprine colostrum, respectively.
33246611	0	111	theme	quality	57:63	arg1	estimation					33:42	Comparative estimation	21:42	Short communication: Comparative estimation of colostrum quality by Brix refractometry in bovine, caprine, and ovine colostrum.	0:126	Short communication: Comparative estimation of colostrum quality by Brix refractometry in bovine, caprine, and ovine colostrum.
33246611	12	112	dep	%	2035:2035	arg1	Se					2037:2038	Se	2037:2038	75% Se	2033:2038	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	12	113	theme	measurements	1862:1873	arg1	accuracy					1845:1852	greatest accuracy	1836:1852	greatest accuracy of Brix measurements	1836:1873	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	4	114	theme	Brix	616:619	arg1	refractometer					621:633	an optical Brix refractometer	605:633	an optical Brix refractometer	605:633	The present study compared bovine, caprine, and ovine colostrum quality estimation using an optical Brix refractometer.
33246611	12	115	theme	87.1	1905:1908	arg1	%					1909:1909	%	1909:1909	%	1909:1909	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
33246611	1	116	contain	containing	187:196	arg2	immunoglobulins					209:223	sufficient immunoglobulins	198:223	sufficient immunoglobulins	198:223	Newborn ungulates depend on the timely supply of colostrum containing sufficient immunoglobulins to obtain passive immunity against disease.
33246611	1	116	contain	containing	187:196	arg1	colostrum					177:185	colostrum	177:185	colostrum containing sufficient immunoglobulins to obtain passive immunity against disease	177:266	Newborn ungulates depend on the timely supply of colostrum containing sufficient immunoglobulins to obtain passive immunity against disease.
33246611	2	117	theme	quality	336:342	arg1	estimation					312:321	a rapid on-farm estimation	296:321	a rapid on-farm estimation of colostrum quality	296:342	Brix refractometry enables a rapid on-farm estimation of colostrum quality and has been intensively studied in bovines.
33246611	9	118	theme	protein	1300:1306	arg1	content					1308:1314	protein content	1300:1314	protein content	1300:1314	Fat and protein content was higher in sheep colostrum compared with the other species, whereas the highest lactose concentrations were detected in goat colostrum.
33246611	0	119	dep	communication	6:18	arg1	estimation					33:42	Comparative estimation	21:42	Short communication: Comparative estimation of colostrum quality by Brix refractometry in bovine, caprine, and ovine colostrum.	0:126	Short communication: Comparative estimation of colostrum quality by Brix refractometry in bovine, caprine, and ovine colostrum.
33246611	12	120	theme	greatest	1836:1843	arg1	accuracy					1845:1852	greatest accuracy	1836:1852	greatest accuracy of Brix measurements	1836:1873	Optimal cutoff points for greatest accuracy of Brix measurements were 19.3% Brix in cows [with 87.1% sensitivity (Se) and 100% specificity (Sp)], 20.7% Brix in does (with 53.5% Se and 100% Sp), and 26.5% Brix in ewes (with 75% Se and 91.3% Sp).
32846509	5	0	theme	10-day-old	692:701	arg1	cultures					703:710	3- and 10-day-old cultures	685:710	3- and 10-day-old cultures that had been grown at 25 °C had D56-values of 1.99 ± 0.15 min and 5.31 ± 1.04 min, respectively	685:807	To this end, cultures were grown for 3, 5, 7 and 10 days at 25 °C or for 7 days at 15, 25 and 30 °C. Conidia of 3- and 10-day-old cultures that had been grown at 25 °C had D56-values of 1.99 ± 0.15 min and 5.31 ± 1.04 min, respectively.
32846509	11	1	theme	resistance	1685:1694	arg1	core					1672:1675	the core	1668:1675	the core of heat resistance of P. roqueforti conidia	1668:1719	Their encoded proteins as well as trehalose and arabitol may form the core of heat resistance of P. roqueforti conidia.
32846509	5	2	theme	cultures	703:710	arg1	Conidia					674:680	15, 25 and 30 °C. Conidia	656:680	15, 25 and 30 °C. Conidia of 3- and 10-day-old cultures that had been grown at 25 °C had D56-values of 1.99 ± 0.15 min and 5.31 ± 1.04 min, respectively	656:807	To this end, cultures were grown for 3, 5, 7 and 10 days at 25 °C or for 7 days at 15, 25 and 30 °C. Conidia of 3- and 10-day-old cultures that had been grown at 25 °C had D56-values of 1.99 ± 0.15 min and 5.31 ± 1.04 min, respectively.
32846509	4	3	theme	thermal	515:521	arg1	resistance					523:532	thermal resistance	515:532	thermal resistance of P. roqueforti conidia	515:557	Here, the effects of cultivation time and temperature on thermal resistance of P. roqueforti conidia were studied.
32846509	7	4	theme	cultivation	1077:1087	arg1	time					1089:1092	cultivation time	1077:1092	cultivation time	1077:1092	Notably, D56-values were not higher when increasing both cultivation time and temperature by growing for 10 days at 30 °C.
32846509	6	5	dep	roqueforti	879:888	arg1	conidia					890:896	P. roqueforti conidia	876:896	P. roqueforti conidia cultured for 7 days at 15 °C and 30 °C	876:935	The effect of cultivation temperature was most pronounced between P. roqueforti conidia cultured for 7 days at 15 °C and 30 °C, where D56-values of 1.12 ± 0.05 min and 4.19 ± 0.11 min were found, respectively.
32846509	0	6	theme	solute	99:104	arg1	composition					106:116	compatible solute composition	88:116	compatible solute composition	88:116	Impact of maturation and growth temperature on cell-size distribution, heat-resistance, compatible solute composition and transcription profiles of Penicillium roqueforti conidia.
32846509	0	7	from	Impact	0:5	arg1	heat-resistance					71:85	heat-resistance	71:85	heat-resistance	71:85	Impact of maturation and growth temperature on cell-size distribution, heat-resistance, compatible solute composition and transcription profiles of Penicillium roqueforti conidia.
32846509	0	7	from	Impact	0:5	arg1	distribution					57:68	cell-size distribution	47:68	cell-size distribution	47:68	Impact of maturation and growth temperature on cell-size distribution, heat-resistance, compatible solute composition and transcription profiles of Penicillium roqueforti conidia.
32846509	0	7	from	Impact	0:5	arg1	composition					106:116	compatible solute composition	88:116	compatible solute composition	88:116	Impact of maturation and growth temperature on cell-size distribution, heat-resistance, compatible solute composition and transcription profiles of Penicillium roqueforti conidia.
32846509	0	7	from	Impact	0:5	arg1	profiles					136:143	transcription profiles	122:143	transcription profiles	122:143	Impact of maturation and growth temperature on cell-size distribution, heat-resistance, compatible solute composition and transcription profiles of Penicillium roqueforti conidia.
32846509	2	8	theme	fungus	299:304	arg1	structures					280:289	the main dispersal structures	261:289	the main dispersal structures of this fungus	261:304	Its conidia are the main dispersal structures of this fungus and therefore the main cause of food contamination.
32846509	2	8	theme	fungus	299:304	arg1	conidia					249:255	Its conidia	245:255	Its conidia	245:255	Its conidia are the main dispersal structures of this fungus and therefore the main cause of food contamination.
32846509	2	8	theme	fungus	299:304	arg1	cause					329:333	therefore the main cause	310:333	therefore the main cause of food contamination	310:355	Its conidia are the main dispersal structures of this fungus and therefore the main cause of food contamination.
32846509	10	9	theme	increased	1572:1580	arg1	temperature					1589:1599	increased growth temperature	1572:1599	increased growth temperature	1572:1599	Only 33 genes were upregulated at both prolonged incubation time and increased growth temperature.
32846509	0	10	theme	compatible	88:97	arg1	composition					106:116	compatible solute composition	88:116	compatible solute composition	88:116	Impact of maturation and growth temperature on cell-size distribution, heat-resistance, compatible solute composition and transcription profiles of Penicillium roqueforti conidia.
32846509	6	11	theme	temperature	836:846	arg1	effect					814:819	The effect	810:819	The effect of cultivation temperature	810:846	The effect of cultivation temperature was most pronounced between P. roqueforti conidia cultured for 7 days at 15 °C and 30 °C, where D56-values of 1.12 ± 0.05 min and 4.19 ± 0.11 min were found, respectively.
32846509	6	11	theme	temperature	836:846	arg1	pronounced					857:866	pronounced	857:866	pronounced	857:866	The effect of cultivation temperature was most pronounced between P. roqueforti conidia cultured for 7 days at 15 °C and 30 °C, where D56-values of 1.12 ± 0.05 min and 4.19 ± 0.11 min were found, respectively.
32846509	9	12	from	conidia	1443:1449	arg1	distinct					1429:1436	distinct	1429:1436	distinct	1429:1436	RNA-sequencing showed that the expression profiles of conidia of 3- to 10-day-old cultures that had been grown at 25 °C were distinct from conidia that had been formed at 15 °C and 30 °C for 7 days.
32846509	9	12	from	conidia	1443:1449	arg1	profiles					1346:1353	the expression profiles	1331:1353	the expression profiles of conidia of 3- to 10-day-old cultures that had been grown at 25 °C	1331:1422	RNA-sequencing showed that the expression profiles of conidia of 3- to 10-day-old cultures that had been grown at 25 °C were distinct from conidia that had been formed at 15 °C and 30 °C for 7 days.
32846509	11	13	theme	heat	1680:1683	arg1	resistance					1685:1694	heat resistance	1680:1694	heat resistance of P. roqueforti conidia	1680:1719	Their encoded proteins as well as trehalose and arabitol may form the core of heat resistance of P. roqueforti conidia.
32846509	6	14	theme	cultivation	824:834	arg1	temperature					836:846	cultivation temperature	824:846	cultivation temperature	824:846	The effect of cultivation temperature was most pronounced between P. roqueforti conidia cultured for 7 days at 15 °C and 30 °C, where D56-values of 1.12 ± 0.05 min and 4.19 ± 0.11 min were found, respectively.
32846509	3	15	theme	heat	442:445	arg1	treatment					447:455	heat treatment	442:455	heat treatment	442:455	These stress resistant asexual spores can be killed by preservation methods such as heat treatment.
32846509	0	16	theme	temperature	32:42	arg1	Impact					0:5	Impact	0:5	Impact of maturation and growth temperature on cell-size distribution, heat-resistance, compatible solute composition and transcription profiles of Penicillium roqueforti conidia.	0:178	Impact of maturation and growth temperature on cell-size distribution, heat-resistance, compatible solute composition and transcription profiles of Penicillium roqueforti conidia.
32846509	10	17	theme	incubation	1552:1561	arg1	time					1563:1566	prolonged incubation time	1542:1566	prolonged incubation time	1542:1566	Only 33 genes were upregulated at both prolonged incubation time and increased growth temperature.
32846509	9	18	theme	10-day-old	1375:1384	arg1	cultures					1386:1393	3- to 10-day-old cultures	1369:1393	3- to 10-day-old cultures that had been grown at 25 °C	1369:1422	RNA-sequencing showed that the expression profiles of conidia of 3- to 10-day-old cultures that had been grown at 25 °C were distinct from conidia that had been formed at 15 °C and 30 °C for 7 days.
32846509	4	19	theme	cultivation	479:489	arg1	effects					468:474	the effects	464:474	the effects of cultivation time and temperature on thermal resistance of P. roqueforti conidia	464:557	Here, the effects of cultivation time and temperature on thermal resistance of P. roqueforti conidia were studied.
32846509	2	20	theme	main	324:327	arg1	cause					329:333	therefore the main cause	310:333	therefore the main cause of food contamination	310:355	Its conidia are the main dispersal structures of this fungus and therefore the main cause of food contamination.
32846509	9	21	theme	cultures	1386:1393	arg1	conidia					1358:1364	conidia	1358:1364	conidia of 3- to 10-day-old cultures that had been grown at 25 °C	1358:1422	RNA-sequencing showed that the expression profiles of conidia of 3- to 10-day-old cultures that had been grown at 25 °C were distinct from conidia that had been formed at 15 °C and 30 °C for 7 days.
32846509	5	22	theme	25 °C	735:739	arg1	D56-values					745:754	25 °C had D56-values	735:754	25 °C had D56-values of 1.99 ± 0.15 min and 5.31 ± 1.04 min, respectively	735:807	To this end, cultures were grown for 3, 5, 7 and 10 days at 25 °C or for 7 days at 15, 25 and 30 °C. Conidia of 3- and 10-day-old cultures that had been grown at 25 °C had D56-values of 1.99 ± 0.15 min and 5.31 ± 1.04 min, respectively.
32846509	11	23	theme	conidia	1713:1719	arg1	resistance					1685:1694	heat resistance	1680:1694	heat resistance of P. roqueforti conidia	1680:1719	Their encoded proteins as well as trehalose and arabitol may form the core of heat resistance of P. roqueforti conidia.
32846509	4	24	from	effects	468:474	arg1	resistance					523:532	thermal resistance	515:532	thermal resistance of P. roqueforti conidia	515:557	Here, the effects of cultivation time and temperature on thermal resistance of P. roqueforti conidia were studied.
32846509	1	25	theme	major	208:212	arg1	roqueforti					192:201	roqueforti	192:201	roqueforti	192:201	Penicillium roqueforti is a major cause of fungal food spoilage.
32846509	1	25	theme	major	208:212	arg1	cause					214:218	a major cause	206:218	a major cause of fungal food spoilage	206:242	Penicillium roqueforti is a major cause of fungal food spoilage.
32846509	3	26	theme	asexual	381:387	arg1	spores					389:394	These stress resistant asexual spores	358:394	These stress resistant asexual spores	358:394	These stress resistant asexual spores can be killed by preservation methods such as heat treatment.
32846509	4	27	theme	conidia	551:557	arg1	resistance					523:532	thermal resistance	515:532	thermal resistance of P. roqueforti conidia	515:557	Here, the effects of cultivation time and temperature on thermal resistance of P. roqueforti conidia were studied.
32846509	10	28	theme	growth	1582:1587	arg1	temperature					1589:1599	increased growth temperature	1572:1599	increased growth temperature	1572:1599	Only 33 genes were upregulated at both prolonged incubation time and increased growth temperature.
32846509	0	29	theme	maturation	10:19	arg1	Impact					0:5	Impact	0:5	Impact of maturation and growth temperature on cell-size distribution, heat-resistance, compatible solute composition and transcription profiles of Penicillium roqueforti conidia.	0:178	Impact of maturation and growth temperature on cell-size distribution, heat-resistance, compatible solute composition and transcription profiles of Penicillium roqueforti conidia.
32846509	11	30	theme	P.	1699:1700	arg1	conidia					1713:1719	P. roqueforti conidia	1699:1719	P. roqueforti conidia	1699:1719	Their encoded proteins as well as trehalose and arabitol may form the core of heat resistance of P. roqueforti conidia.
32846509	0	31	theme	transcription	122:134	arg1	profiles					136:143	transcription profiles	122:143	transcription profiles	122:143	Impact of maturation and growth temperature on cell-size distribution, heat-resistance, compatible solute composition and transcription profiles of Penicillium roqueforti conidia.
32846509	5	32	from	Conidia	674:680	arg1	25 °C					633:637	25 °C	633:637	25 °C	633:637	To this end, cultures were grown for 3, 5, 7 and 10 days at 25 °C or for 7 days at 15, 25 and 30 °C. Conidia of 3- and 10-day-old cultures that had been grown at 25 °C had D56-values of 1.99 ± 0.15 min and 5.31 ± 1.04 min, respectively.
32846509	5	33	theme	1.99 ± 0.15 min	759:773	arg1	D56-values					745:754	25 °C had D56-values	735:754	25 °C had D56-values of 1.99 ± 0.15 min and 5.31 ± 1.04 min, respectively	735:807	To this end, cultures were grown for 3, 5, 7 and 10 days at 25 °C or for 7 days at 15, 25 and 30 °C. Conidia of 3- and 10-day-old cultures that had been grown at 25 °C had D56-values of 1.99 ± 0.15 min and 5.31 ± 1.04 min, respectively.
32846509	3	34	theme	preservation	413:424	arg1	treatment					447:455	heat treatment	442:455	heat treatment	442:455	These stress resistant asexual spores can be killed by preservation methods such as heat treatment.
32846509	3	34	theme	preservation	413:424	arg1	methods					426:432	preservation methods	413:432	preservation methods such as heat treatment	413:455	These stress resistant asexual spores can be killed by preservation methods such as heat treatment.
32846509	8	35	theme	arabitol	1230:1237	arg1	resistance					1180:1189	heat resistance	1175:1189	heat resistance of conidia	1175:1200	A correlation was found between heat resistance of conidia and levels of trehalose and arabitol, while this was not found for glycerol, mannitol and erythritol.
32846509	8	35	theme	arabitol	1230:1237	arg1	levels					1206:1211	levels	1206:1211	levels of trehalose and arabitol	1206:1237	A correlation was found between heat resistance of conidia and levels of trehalose and arabitol, while this was not found for glycerol, mannitol and erythritol.
32846509	8	36	theme	conidia	1194:1200	arg1	resistance					1180:1189	heat resistance	1175:1189	heat resistance of conidia	1175:1200	A correlation was found between heat resistance of conidia and levels of trehalose and arabitol, while this was not found for glycerol, mannitol and erythritol.
32846509	8	36	theme	conidia	1194:1200	arg1	levels					1206:1211	levels	1206:1211	levels of trehalose and arabitol	1206:1237	A correlation was found between heat resistance of conidia and levels of trehalose and arabitol, while this was not found for glycerol, mannitol and erythritol.
32846509	1	37	theme	fungal	223:228	arg1	spoilage					235:242	fungal food spoilage	223:242	fungal food spoilage	223:242	Penicillium roqueforti is a major cause of fungal food spoilage.
32846509	0	38	theme	growth	25:30	arg1	temperature					32:42	growth temperature	25:42	growth temperature	25:42	Impact of maturation and growth temperature on cell-size distribution, heat-resistance, compatible solute composition and transcription profiles of Penicillium roqueforti conidia.
32846509	9	39	theme	conidia	1358:1364	arg1	distinct					1429:1436	distinct	1429:1436	distinct	1429:1436	RNA-sequencing showed that the expression profiles of conidia of 3- to 10-day-old cultures that had been grown at 25 °C were distinct from conidia that had been formed at 15 °C and 30 °C for 7 days.
32846509	9	39	theme	conidia	1358:1364	arg1	profiles					1346:1353	the expression profiles	1331:1353	the expression profiles of conidia of 3- to 10-day-old cultures that had been grown at 25 °C	1331:1422	RNA-sequencing showed that the expression profiles of conidia of 3- to 10-day-old cultures that had been grown at 25 °C were distinct from conidia that had been formed at 15 °C and 30 °C for 7 days.
32846509	11	40	theme	encoded	1608:1614	arg1	proteins					1616:1623	Their encoded proteins	1602:1623	Their encoded proteins as well as trehalose and arabitol	1602:1657	Their encoded proteins as well as trehalose and arabitol may form the core of heat resistance of P. roqueforti conidia.
32846509	5	41	theme	had	741:743	arg1	D56-values					745:754	25 °C had D56-values	735:754	25 °C had D56-values of 1.99 ± 0.15 min and 5.31 ± 1.04 min, respectively	735:807	To this end, cultures were grown for 3, 5, 7 and 10 days at 25 °C or for 7 days at 15, 25 and 30 °C. Conidia of 3- and 10-day-old cultures that had been grown at 25 °C had D56-values of 1.99 ± 0.15 min and 5.31 ± 1.04 min, respectively.
32846509	1	42	theme	food	230:233	arg1	spoilage					235:242	fungal food spoilage	223:242	fungal food spoilage	223:242	Penicillium roqueforti is a major cause of fungal food spoilage.
32846509	0	43	theme	cell-size	47:55	arg1	distribution					57:68	cell-size distribution	47:68	cell-size distribution	47:68	Impact of maturation and growth temperature on cell-size distribution, heat-resistance, compatible solute composition and transcription profiles of Penicillium roqueforti conidia.
32846509	3	44	theme	stress	364:369	arg1	spores					389:394	These stress resistant asexual spores	358:394	These stress resistant asexual spores	358:394	These stress resistant asexual spores can be killed by preservation methods such as heat treatment.
32846509	6	45	theme	4.19 ± 0.11 min	978:992	arg1	D56-values					944:953	D56-values	944:953	D56-values of 1.12 ± 0.05 min and 4.19 ± 0.11 min	944:992	The effect of cultivation temperature was most pronounced between P. roqueforti conidia cultured for 7 days at 15 °C and 30 °C, where D56-values of 1.12 ± 0.05 min and 4.19 ± 0.11 min were found, respectively.
32846509	4	46	dep	cultivation	479:489	arg1	time					491:494	time	491:494	time	491:494	Here, the effects of cultivation time and temperature on thermal resistance of P. roqueforti conidia were studied.
32846509	2	47	theme	contamination	343:355	arg1	structures					280:289	the main dispersal structures	261:289	the main dispersal structures of this fungus	261:304	Its conidia are the main dispersal structures of this fungus and therefore the main cause of food contamination.
32846509	2	47	theme	contamination	343:355	arg1	conidia					249:255	Its conidia	245:255	Its conidia	245:255	Its conidia are the main dispersal structures of this fungus and therefore the main cause of food contamination.
32846509	2	47	theme	contamination	343:355	arg1	cause					329:333	therefore the main cause	310:333	therefore the main cause of food contamination	310:355	Its conidia are the main dispersal structures of this fungus and therefore the main cause of food contamination.
32846509	4	48	theme	roqueforti	540:549	arg1	conidia					551:557	P. roqueforti conidia	537:557	P. roqueforti conidia	537:557	Here, the effects of cultivation time and temperature on thermal resistance of P. roqueforti conidia were studied.
32846509	0	49	theme	roqueforti	160:169	arg1	heat-resistance					71:85	heat-resistance	71:85	heat-resistance	71:85	Impact of maturation and growth temperature on cell-size distribution, heat-resistance, compatible solute composition and transcription profiles of Penicillium roqueforti conidia.
32846509	0	49	theme	roqueforti	160:169	arg1	distribution					57:68	cell-size distribution	47:68	cell-size distribution	47:68	Impact of maturation and growth temperature on cell-size distribution, heat-resistance, compatible solute composition and transcription profiles of Penicillium roqueforti conidia.
32846509	0	49	theme	roqueforti	160:169	arg1	composition					106:116	compatible solute composition	88:116	compatible solute composition	88:116	Impact of maturation and growth temperature on cell-size distribution, heat-resistance, compatible solute composition and transcription profiles of Penicillium roqueforti conidia.
32846509	0	49	theme	roqueforti	160:169	arg1	profiles					136:143	transcription profiles	122:143	transcription profiles	122:143	Impact of maturation and growth temperature on cell-size distribution, heat-resistance, compatible solute composition and transcription profiles of Penicillium roqueforti conidia.
32846509	9	50	dep	10-day-old	1375:1384	arg1	to					1372:1373	to	1372:1373	to	1372:1373	RNA-sequencing showed that the expression profiles of conidia of 3- to 10-day-old cultures that had been grown at 25 °C were distinct from conidia that had been formed at 15 °C and 30 °C for 7 days.
32846509	2	51	theme	food	338:341	arg1	contamination					343:355	food contamination	338:355	food contamination	338:355	Its conidia are the main dispersal structures of this fungus and therefore the main cause of food contamination.
32846509	4	52	theme	P.	537:538	arg1	conidia					551:557	P. roqueforti conidia	537:557	P. roqueforti conidia	537:557	Here, the effects of cultivation time and temperature on thermal resistance of P. roqueforti conidia were studied.
32846509	10	53	theme	prolonged	1542:1550	arg1	time					1563:1566	prolonged incubation time	1542:1566	prolonged incubation time	1542:1566	Only 33 genes were upregulated at both prolonged incubation time and increased growth temperature.
32846509	11	54	theme	roqueforti	1702:1711	arg1	conidia					1713:1719	P. roqueforti conidia	1699:1719	P. roqueforti conidia	1699:1719	Their encoded proteins as well as trehalose and arabitol may form the core of heat resistance of P. roqueforti conidia.
32846509	2	55	theme	dispersal	270:278	arg1	structures					280:289	the main dispersal structures	261:289	the main dispersal structures of this fungus	261:304	Its conidia are the main dispersal structures of this fungus and therefore the main cause of food contamination.
32846509	2	55	theme	dispersal	270:278	arg1	conidia					249:255	Its conidia	245:255	Its conidia	245:255	Its conidia are the main dispersal structures of this fungus and therefore the main cause of food contamination.
32846509	1	56	theme	spoilage	235:242	arg1	roqueforti					192:201	roqueforti	192:201	roqueforti	192:201	Penicillium roqueforti is a major cause of fungal food spoilage.
32846509	1	56	theme	spoilage	235:242	arg1	cause					214:218	a major cause	206:218	a major cause of fungal food spoilage	206:242	Penicillium roqueforti is a major cause of fungal food spoilage.
32846509	2	57	theme	main	265:268	arg1	structures					280:289	the main dispersal structures	261:289	the main dispersal structures of this fungus	261:304	Its conidia are the main dispersal structures of this fungus and therefore the main cause of food contamination.
32846509	2	57	theme	main	265:268	arg1	conidia					249:255	Its conidia	245:255	Its conidia	245:255	Its conidia are the main dispersal structures of this fungus and therefore the main cause of food contamination.
32846509	0	58	dep	roqueforti	160:169	arg1	conidia					171:177	Penicillium roqueforti conidia	148:177	Penicillium roqueforti conidia	148:177	Impact of maturation and growth temperature on cell-size distribution, heat-resistance, compatible solute composition and transcription profiles of Penicillium roqueforti conidia.
32846509	6	59	theme	1.12 ± 0.05 min	958:972	arg1	D56-values					944:953	D56-values	944:953	D56-values of 1.12 ± 0.05 min and 4.19 ± 0.11 min	944:992	The effect of cultivation temperature was most pronounced between P. roqueforti conidia cultured for 7 days at 15 °C and 30 °C, where D56-values of 1.12 ± 0.05 min and 4.19 ± 0.11 min were found, respectively.
32846509	5	60	theme	3-	685:686	arg1	cultures					703:710	3- and 10-day-old cultures	685:710	3- and 10-day-old cultures that had been grown at 25 °C had D56-values of 1.99 ± 0.15 min and 5.31 ± 1.04 min, respectively	685:807	To this end, cultures were grown for 3, 5, 7 and 10 days at 25 °C or for 7 days at 15, 25 and 30 °C. Conidia of 3- and 10-day-old cultures that had been grown at 25 °C had D56-values of 1.99 ± 0.15 min and 5.31 ± 1.04 min, respectively.
32846509	5	61	theme	5.31 ± 1.04 min	779:793	arg1	D56-values					745:754	25 °C had D56-values	735:754	25 °C had D56-values of 1.99 ± 0.15 min and 5.31 ± 1.04 min, respectively	735:807	To this end, cultures were grown for 3, 5, 7 and 10 days at 25 °C or for 7 days at 15, 25 and 30 °C. Conidia of 3- and 10-day-old cultures that had been grown at 25 °C had D56-values of 1.99 ± 0.15 min and 5.31 ± 1.04 min, respectively.
32846509	8	62	theme	trehalose	1216:1224	arg1	resistance					1180:1189	heat resistance	1175:1189	heat resistance of conidia	1175:1200	A correlation was found between heat resistance of conidia and levels of trehalose and arabitol, while this was not found for glycerol, mannitol and erythritol.
32846509	8	62	theme	trehalose	1216:1224	arg1	levels					1206:1211	levels	1206:1211	levels of trehalose and arabitol	1206:1237	A correlation was found between heat resistance of conidia and levels of trehalose and arabitol, while this was not found for glycerol, mannitol and erythritol.
32846509	9	63	theme	expression	1335:1344	arg1	distinct					1429:1436	distinct	1429:1436	distinct	1429:1436	RNA-sequencing showed that the expression profiles of conidia of 3- to 10-day-old cultures that had been grown at 25 °C were distinct from conidia that had been formed at 15 °C and 30 °C for 7 days.
32846509	9	63	theme	expression	1335:1344	arg1	profiles					1346:1353	the expression profiles	1331:1353	the expression profiles of conidia of 3- to 10-day-old cultures that had been grown at 25 °C	1331:1422	RNA-sequencing showed that the expression profiles of conidia of 3- to 10-day-old cultures that had been grown at 25 °C were distinct from conidia that had been formed at 15 °C and 30 °C for 7 days.
32846509	8	64	theme	heat	1175:1178	arg1	resistance					1180:1189	heat resistance	1175:1189	heat resistance of conidia	1175:1200	A correlation was found between heat resistance of conidia and levels of trehalose and arabitol, while this was not found for glycerol, mannitol and erythritol.
32846509	4	65	theme	temperature	500:510	arg1	effects					468:474	the effects	464:474	the effects of cultivation time and temperature on thermal resistance of P. roqueforti conidia	464:557	Here, the effects of cultivation time and temperature on thermal resistance of P. roqueforti conidia were studied.
32846509	3	66	theme	resistant	371:379	arg1	spores					389:394	These stress resistant asexual spores	358:394	These stress resistant asexual spores	358:394	These stress resistant asexual spores can be killed by preservation methods such as heat treatment.
32739510	2	0	theme	bactericidal	479:490	arg1	activity					492:499	their bacteriostatic and bactericidal activity	454:499	their bacteriostatic and bactericidal activity	454:499	PAC-CHT-GEN NPs were evaluated for their ability to agglutinate E. coli, S. aureus, and P. aeruginosa and their bacteriostatic and bactericidal activity.
32739510	6	1	dep	suggested	944:952	arg1	form					970:973	form	970:973	suggested PAC-CHT-GEN NPs form stable, round-shaped, and bioactive nanoparticles with the potential to be use in the treatment of bacterial infections	944:1093	Results suggested PAC-CHT-GEN NPs form stable, round-shaped, and bioactive nanoparticles with the potential to be use in the treatment of bacterial infections.
32739510	1	2	theme	Cranberry	88:96	arg1	nanoparticles					124:136	Cranberry proanthocyanidin-chitosan nanoparticles	88:136	Cranberry proanthocyanidin-chitosan nanoparticles (PAC-CHT NPs) loaded with antibiotic gentamicin (GEN) (PAC-CHT-GEN NPs)	88:208	Cranberry proanthocyanidin-chitosan nanoparticles (PAC-CHT NPs) loaded with antibiotic gentamicin (GEN) (PAC-CHT-GEN NPs) were formulated and characterized according to size, polydispersity (PDI), surface charge, morphology, and encapsulation efficiency (EE).
32739510	1	2	theme	Cranberry	88:96	arg1	NPs					147:149	PAC-CHT NPs	139:149	PAC-CHT NPs	139:149	Cranberry proanthocyanidin-chitosan nanoparticles (PAC-CHT NPs) loaded with antibiotic gentamicin (GEN) (PAC-CHT-GEN NPs) were formulated and characterized according to size, polydispersity (PDI), surface charge, morphology, and encapsulation efficiency (EE).
32739510	6	3	theme	infections	1084:1093	arg1	treatment					1061:1069	the treatment	1057:1069	the treatment of bacterial infections	1057:1093	Results suggested PAC-CHT-GEN NPs form stable, round-shaped, and bioactive nanoparticles with the potential to be use in the treatment of bacterial infections.
32739510	3	4	theme	zeta	687:690	arg1	potential					692:700	a zeta potential	685:700	a zeta potential from 34.5	685:710	Results indicate that the PAC-CHT-GEN NPs at 0.5:1.0, 1.0:1.0, and 2.0:1.0 weight ratios formed stable nanoparticles with sizes from 242.9 to 277.4 nm, a PDI from 0.344 to 0.391, and a zeta potential from 34.5 to 38.5 mV, and up to 94% EE.
32739510	5	5	theme	greater	890:896	arg1	activity					911:918	greater bactericidal activity	890:918	greater bactericidal activity	890:918	Furthermore, PAC-CHT-GEN NPs exhibited greater bactericidal activity than GEN alone.
32739510	1	6	theme	proanthocyanidin-chitosan	98:122	arg1	nanoparticles					124:136	Cranberry proanthocyanidin-chitosan nanoparticles	88:136	Cranberry proanthocyanidin-chitosan nanoparticles (PAC-CHT NPs) loaded with antibiotic gentamicin (GEN) (PAC-CHT-GEN NPs)	88:208	Cranberry proanthocyanidin-chitosan nanoparticles (PAC-CHT NPs) loaded with antibiotic gentamicin (GEN) (PAC-CHT-GEN NPs) were formulated and characterized according to size, polydispersity (PDI), surface charge, morphology, and encapsulation efficiency (EE).
32739510	1	6	theme	proanthocyanidin-chitosan	98:122	arg1	NPs					147:149	PAC-CHT NPs	139:149	PAC-CHT NPs	139:149	Cranberry proanthocyanidin-chitosan nanoparticles (PAC-CHT NPs) loaded with antibiotic gentamicin (GEN) (PAC-CHT-GEN NPs) were formulated and characterized according to size, polydispersity (PDI), surface charge, morphology, and encapsulation efficiency (EE).
32739510	1	7	theme	surface	285:291	arg1	charge					293:298	surface charge	285:298	surface charge	285:298	Cranberry proanthocyanidin-chitosan nanoparticles (PAC-CHT NPs) loaded with antibiotic gentamicin (GEN) (PAC-CHT-GEN NPs) were formulated and characterized according to size, polydispersity (PDI), surface charge, morphology, and encapsulation efficiency (EE).
32739510	3	8	theme	PAC-CHT-GEN	528:538	arg1	NPs					540:542	the PAC-CHT-GEN NPs	524:542	the PAC-CHT-GEN NPs at 0.5:1.0, 1.0:1.0, and 2.0:1.0 weight ratios	524:589	Results indicate that the PAC-CHT-GEN NPs at 0.5:1.0, 1.0:1.0, and 2.0:1.0 weight ratios formed stable nanoparticles with sizes from 242.9 to 277.4 nm, a PDI from 0.344 to 0.391, and a zeta potential from 34.5 to 38.5 mV, and up to 94% EE.
32739510	3	9	from	277.4 nm	644:651	arg1	sizes					624:628	sizes	624:628	sizes from 242.9 to 277.4 nm	624:651	Results indicate that the PAC-CHT-GEN NPs at 0.5:1.0, 1.0:1.0, and 2.0:1.0 weight ratios formed stable nanoparticles with sizes from 242.9 to 277.4 nm, a PDI from 0.344 to 0.391, and a zeta potential from 34.5 to 38.5 mV, and up to 94% EE.
32739510	3	10	from	34.5	707:710	arg1	nanoparticles					605:617	stable nanoparticles	598:617	stable nanoparticles with sizes from 242.9 to 277.4 nm	598:651	Results indicate that the PAC-CHT-GEN NPs at 0.5:1.0, 1.0:1.0, and 2.0:1.0 weight ratios formed stable nanoparticles with sizes from 242.9 to 277.4 nm, a PDI from 0.344 to 0.391, and a zeta potential from 34.5 to 38.5 mV, and up to 94% EE.
32739510	3	10	from	34.5	707:710	arg1	PDI					656:658	a PDI	654:658	a PDI from 0.344 to 0.391	654:678	Results indicate that the PAC-CHT-GEN NPs at 0.5:1.0, 1.0:1.0, and 2.0:1.0 weight ratios formed stable nanoparticles with sizes from 242.9 to 277.4 nm, a PDI from 0.344 to 0.391, and a zeta potential from 34.5 to 38.5 mV, and up to 94% EE.
32739510	3	10	from	34.5	707:710	arg1	potential					692:700	a zeta potential	685:700	a zeta potential from 34.5	685:710	Results indicate that the PAC-CHT-GEN NPs at 0.5:1.0, 1.0:1.0, and 2.0:1.0 weight ratios formed stable nanoparticles with sizes from 242.9 to 277.4 nm, a PDI from 0.344 to 0.391, and a zeta potential from 34.5 to 38.5 mV, and up to 94% EE.
32739510	3	11	with	PDI	656:658	arg1	sizes					624:628	sizes	624:628	sizes from 242.9 to 277.4 nm	624:651	Results indicate that the PAC-CHT-GEN NPs at 0.5:1.0, 1.0:1.0, and 2.0:1.0 weight ratios formed stable nanoparticles with sizes from 242.9 to 277.4 nm, a PDI from 0.344 to 0.391, and a zeta potential from 34.5 to 38.5 mV, and up to 94% EE.
32739510	6	12	theme	bioactive	1001:1009	arg1	nanoparticles					1011:1023	stable, round-shaped, and bioactive nanoparticles	975:1023	stable, round-shaped, and bioactive nanoparticles	975:1023	Results suggested PAC-CHT-GEN NPs form stable, round-shaped, and bioactive nanoparticles with the potential to be use in the treatment of bacterial infections.
32739510	0	13	theme	proanthocyanidin-chitosan	14:38	arg1	composite					40:48	Antimicrobial proanthocyanidin-chitosan composite	0:48	Antimicrobial proanthocyanidin-chitosan composite	0:48	Antimicrobial proanthocyanidin-chitosan composite nanoparticles loaded with gentamicin.
32739510	1	14	theme	PAC-CHT	139:145	arg1	nanoparticles					124:136	Cranberry proanthocyanidin-chitosan nanoparticles	88:136	Cranberry proanthocyanidin-chitosan nanoparticles (PAC-CHT NPs) loaded with antibiotic gentamicin (GEN) (PAC-CHT-GEN NPs)	88:208	Cranberry proanthocyanidin-chitosan nanoparticles (PAC-CHT NPs) loaded with antibiotic gentamicin (GEN) (PAC-CHT-GEN NPs) were formulated and characterized according to size, polydispersity (PDI), surface charge, morphology, and encapsulation efficiency (EE).
32739510	1	14	theme	PAC-CHT	139:145	arg1	NPs					147:149	PAC-CHT NPs	139:149	PAC-CHT NPs	139:149	Cranberry proanthocyanidin-chitosan nanoparticles (PAC-CHT NPs) loaded with antibiotic gentamicin (GEN) (PAC-CHT-GEN NPs) were formulated and characterized according to size, polydispersity (PDI), surface charge, morphology, and encapsulation efficiency (EE).
32739510	0	15	theme	Antimicrobial	0:12	arg1	composite					40:48	Antimicrobial proanthocyanidin-chitosan composite	0:48	Antimicrobial proanthocyanidin-chitosan composite	0:48	Antimicrobial proanthocyanidin-chitosan composite nanoparticles loaded with gentamicin.
32739510	6	16	theme	bacterial	1074:1082	arg1	infections					1084:1093	bacterial infections	1074:1093	bacterial infections	1074:1093	Results suggested PAC-CHT-GEN NPs form stable, round-shaped, and bioactive nanoparticles with the potential to be use in the treatment of bacterial infections.
32739510	5	17	theme	PAC-CHT-GEN	864:874	arg1	NPs					876:878	PAC-CHT-GEN NPs	864:878	PAC-CHT-GEN NPs	864:878	Furthermore, PAC-CHT-GEN NPs exhibited greater bactericidal activity than GEN alone.
32739510	6	18	theme	round-shaped	983:994	arg1	nanoparticles					1011:1023	stable, round-shaped, and bioactive nanoparticles	975:1023	stable, round-shaped, and bioactive nanoparticles	975:1023	Results suggested PAC-CHT-GEN NPs form stable, round-shaped, and bioactive nanoparticles with the potential to be use in the treatment of bacterial infections.
32739510	3	19	dep	0.391	674:678	arg1	to					671:672	to	671:672	to	671:672	Results indicate that the PAC-CHT-GEN NPs at 0.5:1.0, 1.0:1.0, and 2.0:1.0 weight ratios formed stable nanoparticles with sizes from 242.9 to 277.4 nm, a PDI from 0.344 to 0.391, and a zeta potential from 34.5 to 38.5 mV, and up to 94% EE.
32739510	5	20	theme	bactericidal	898:909	arg1	activity					911:918	greater bactericidal activity	890:918	greater bactericidal activity	890:918	Furthermore, PAC-CHT-GEN NPs exhibited greater bactericidal activity than GEN alone.
32739510	3	21	dep	94	734:735	arg1	to					731:732	to	731:732	to	731:732	Results indicate that the PAC-CHT-GEN NPs at 0.5:1.0, 1.0:1.0, and 2.0:1.0 weight ratios formed stable nanoparticles with sizes from 242.9 to 277.4 nm, a PDI from 0.344 to 0.391, and a zeta potential from 34.5 to 38.5 mV, and up to 94% EE.
32739510	3	22	from	ratios	584:589	arg1	NPs					540:542	the PAC-CHT-GEN NPs	524:542	the PAC-CHT-GEN NPs at 0.5:1.0, 1.0:1.0, and 2.0:1.0 weight ratios	524:589	Results indicate that the PAC-CHT-GEN NPs at 0.5:1.0, 1.0:1.0, and 2.0:1.0 weight ratios formed stable nanoparticles with sizes from 242.9 to 277.4 nm, a PDI from 0.344 to 0.391, and a zeta potential from 34.5 to 38.5 mV, and up to 94% EE.
32739510	2	23	theme	bacteriostatic	460:473	arg1	activity					492:499	their bacteriostatic and bactericidal activity	454:499	their bacteriostatic and bactericidal activity	454:499	PAC-CHT-GEN NPs were evaluated for their ability to agglutinate E. coli, S. aureus, and P. aeruginosa and their bacteriostatic and bactericidal activity.
32739510	3	24	dep	%	736:736	arg1	94					734:735	94	734:735	94	734:735	Results indicate that the PAC-CHT-GEN NPs at 0.5:1.0, 1.0:1.0, and 2.0:1.0 weight ratios formed stable nanoparticles with sizes from 242.9 to 277.4 nm, a PDI from 0.344 to 0.391, and a zeta potential from 34.5 to 38.5 mV, and up to 94% EE.
32739510	4	25	contain	have	780:783	arg1	NPs					776:778	PAC-CHT-GEN NPs	764:778	PAC-CHT-GEN NPs	764:778	Results indicate that PAC-CHT-GEN NPs have the ability to agglutinate E. coli, S. aureus, and P. aeruginosa.
32739510	4	25	contain	have	780:783	arg2	ability					789:795	the ability to agglutinate E. coli, S. aureus, and P. aeruginosa	785:848	the ability to agglutinate E. coli, S. aureus, and P. aeruginosa	785:848	Results indicate that PAC-CHT-GEN NPs have the ability to agglutinate E. coli, S. aureus, and P. aeruginosa.
32739510	6	26	theme	stable	975:980	arg1	nanoparticles					1011:1023	stable, round-shaped, and bioactive nanoparticles	975:1023	stable, round-shaped, and bioactive nanoparticles	975:1023	Results suggested PAC-CHT-GEN NPs form stable, round-shaped, and bioactive nanoparticles with the potential to be use in the treatment of bacterial infections.
32739510	3	27	dep	EE	738:739	arg1	%					736:736	%	736:736	%	736:736	Results indicate that the PAC-CHT-GEN NPs at 0.5:1.0, 1.0:1.0, and 2.0:1.0 weight ratios formed stable nanoparticles with sizes from 242.9 to 277.4 nm, a PDI from 0.344 to 0.391, and a zeta potential from 34.5 to 38.5 mV, and up to 94% EE.
32739510	6	28	from	use	1050:1052	arg1	treatment					1061:1069	the treatment	1057:1069	the treatment of bacterial infections	1057:1093	Results suggested PAC-CHT-GEN NPs form stable, round-shaped, and bioactive nanoparticles with the potential to be use in the treatment of bacterial infections.
32739510	3	29	from	0.391	674:678	arg1	nanoparticles					605:617	stable nanoparticles	598:617	stable nanoparticles with sizes from 242.9 to 277.4 nm	598:651	Results indicate that the PAC-CHT-GEN NPs at 0.5:1.0, 1.0:1.0, and 2.0:1.0 weight ratios formed stable nanoparticles with sizes from 242.9 to 277.4 nm, a PDI from 0.344 to 0.391, and a zeta potential from 34.5 to 38.5 mV, and up to 94% EE.
32739510	3	29	from	0.391	674:678	arg1	PDI					656:658	a PDI	654:658	a PDI from 0.344 to 0.391	654:678	Results indicate that the PAC-CHT-GEN NPs at 0.5:1.0, 1.0:1.0, and 2.0:1.0 weight ratios formed stable nanoparticles with sizes from 242.9 to 277.4 nm, a PDI from 0.344 to 0.391, and a zeta potential from 34.5 to 38.5 mV, and up to 94% EE.
32739510	3	29	from	0.391	674:678	arg1	potential					692:700	a zeta potential	685:700	a zeta potential from 34.5	685:710	Results indicate that the PAC-CHT-GEN NPs at 0.5:1.0, 1.0:1.0, and 2.0:1.0 weight ratios formed stable nanoparticles with sizes from 242.9 to 277.4 nm, a PDI from 0.344 to 0.391, and a zeta potential from 34.5 to 38.5 mV, and up to 94% EE.
32739510	1	30	theme	encapsulation	317:329	arg1	EE					343:344	EE	343:344	EE	343:344	Cranberry proanthocyanidin-chitosan nanoparticles (PAC-CHT NPs) loaded with antibiotic gentamicin (GEN) (PAC-CHT-GEN NPs) were formulated and characterized according to size, polydispersity (PDI), surface charge, morphology, and encapsulation efficiency (EE).
32739510	1	30	theme	encapsulation	317:329	arg1	efficiency					331:340	encapsulation efficiency	317:340	encapsulation efficiency (EE)	317:345	Cranberry proanthocyanidin-chitosan nanoparticles (PAC-CHT NPs) loaded with antibiotic gentamicin (GEN) (PAC-CHT-GEN NPs) were formulated and characterized according to size, polydispersity (PDI), surface charge, morphology, and encapsulation efficiency (EE).
32739510	4	31	theme	PAC-CHT-GEN	764:774	arg1	NPs					776:778	PAC-CHT-GEN NPs	764:778	PAC-CHT-GEN NPs	764:778	Results indicate that PAC-CHT-GEN NPs have the ability to agglutinate E. coli, S. aureus, and P. aeruginosa.
32739510	2	32	theme	PAC-CHT-GEN	348:358	arg1	NPs					360:362	PAC-CHT-GEN NPs	348:362	PAC-CHT-GEN NPs	348:362	PAC-CHT-GEN NPs were evaluated for their ability to agglutinate E. coli, S. aureus, and P. aeruginosa and their bacteriostatic and bactericidal activity.
32739510	3	33	theme	weight	577:582	arg1	ratios					584:589	0.5:1.0, 1.0:1.0, and 2.0:1.0 weight ratios	547:589	0.5:1.0, 1.0:1.0, and 2.0:1.0 weight ratios	547:589	Results indicate that the PAC-CHT-GEN NPs at 0.5:1.0, 1.0:1.0, and 2.0:1.0 weight ratios formed stable nanoparticles with sizes from 242.9 to 277.4 nm, a PDI from 0.344 to 0.391, and a zeta potential from 34.5 to 38.5 mV, and up to 94% EE.
32739510	1	34	theme	antibiotic	164:173	arg1	gentamicin					175:184	antibiotic gentamicin	164:184	antibiotic gentamicin (GEN) (PAC-CHT-GEN NPs)	164:208	Cranberry proanthocyanidin-chitosan nanoparticles (PAC-CHT NPs) loaded with antibiotic gentamicin (GEN) (PAC-CHT-GEN NPs) were formulated and characterized according to size, polydispersity (PDI), surface charge, morphology, and encapsulation efficiency (EE).
32739510	1	34	theme	antibiotic	164:173	arg1	NPs					205:207	PAC-CHT-GEN NPs	193:207	PAC-CHT-GEN NPs	193:207	Cranberry proanthocyanidin-chitosan nanoparticles (PAC-CHT NPs) loaded with antibiotic gentamicin (GEN) (PAC-CHT-GEN NPs) were formulated and characterized according to size, polydispersity (PDI), surface charge, morphology, and encapsulation efficiency (EE).
32739510	1	34	theme	antibiotic	164:173	arg1	GEN					187:189	GEN	187:189	GEN	187:189	Cranberry proanthocyanidin-chitosan nanoparticles (PAC-CHT NPs) loaded with antibiotic gentamicin (GEN) (PAC-CHT-GEN NPs) were formulated and characterized according to size, polydispersity (PDI), surface charge, morphology, and encapsulation efficiency (EE).
32739510	0	35	dep	nanoparticles	50:62	arg1	loaded					64:69	loaded	64:69	nanoparticles loaded with gentamicin	50:85	Antimicrobial proanthocyanidin-chitosan composite nanoparticles loaded with gentamicin.
32739510	6	36	theme	PAC-CHT-GEN	954:964	arg1	NPs					966:968	PAC-CHT-GEN NPs	954:968	PAC-CHT-GEN NPs	954:968	Results suggested PAC-CHT-GEN NPs form stable, round-shaped, and bioactive nanoparticles with the potential to be use in the treatment of bacterial infections.
32739510	3	37	theme	stable	598:603	arg1	nanoparticles					605:617	stable nanoparticles	598:617	stable nanoparticles with sizes from 242.9 to 277.4 nm	598:651	Results indicate that the PAC-CHT-GEN NPs at 0.5:1.0, 1.0:1.0, and 2.0:1.0 weight ratios formed stable nanoparticles with sizes from 242.9 to 277.4 nm, a PDI from 0.344 to 0.391, and a zeta potential from 34.5 to 38.5 mV, and up to 94% EE.
32739510	3	38	with	potential	692:700	arg1	sizes					624:628	sizes	624:628	sizes from 242.9 to 277.4 nm	624:651	Results indicate that the PAC-CHT-GEN NPs at 0.5:1.0, 1.0:1.0, and 2.0:1.0 weight ratios formed stable nanoparticles with sizes from 242.9 to 277.4 nm, a PDI from 0.344 to 0.391, and a zeta potential from 34.5 to 38.5 mV, and up to 94% EE.
32739510	3	39	with	nanoparticles	605:617	arg1	sizes					624:628	sizes	624:628	sizes from 242.9 to 277.4 nm	624:651	Results indicate that the PAC-CHT-GEN NPs at 0.5:1.0, 1.0:1.0, and 2.0:1.0 weight ratios formed stable nanoparticles with sizes from 242.9 to 277.4 nm, a PDI from 0.344 to 0.391, and a zeta potential from 34.5 to 38.5 mV, and up to 94% EE.
32739510	3	40	dep	277.4 nm	644:651	arg1	to					641:642	to	641:642	to	641:642	Results indicate that the PAC-CHT-GEN NPs at 0.5:1.0, 1.0:1.0, and 2.0:1.0 weight ratios formed stable nanoparticles with sizes from 242.9 to 277.4 nm, a PDI from 0.344 to 0.391, and a zeta potential from 34.5 to 38.5 mV, and up to 94% EE.
32669583	0	0	theme	cellulose	74:82	arg1	nanofibers					84:93	cellulose nanofibers	74:93	cellulose nanofibers	74:93	Valorization of Colombian fique (Furcraea bedinghausii) for production of cellulose nanofibers and its application in hydrogels.
32669583	3	1	theme	stability	647:655	arg1	analysis					657:664	thermal stability analysis	639:664	thermal stability analysis	639:664	The raw material (NAT-F), bleached fibers (B-F), hydrolyzed fibers and fibers treated with ultrasound (US-F) were characterized by infrared spectroscopy (FTIR) and thermal stability analysis; also, in order to have a comparison criterion, a commercial microcrystalline cellulose sample (CC) was analyzed, which demonstrated the extraction of fique cellulose.
32669583	3	2	dep	order	676:680	arg1	have					685:688	have	685:688	to have a comparison criterion	682:711	The raw material (NAT-F), bleached fibers (B-F), hydrolyzed fibers and fibers treated with ultrasound (US-F) were characterized by infrared spectroscopy (FTIR) and thermal stability analysis; also, in order to have a comparison criterion, a commercial microcrystalline cellulose sample (CC) was analyzed, which demonstrated the extraction of fique cellulose.
32669583	5	3	theme	strain	1044:1049	arg1	percent					1051:1057	the strain percent	1040:1057	the strain percent	1040:1057	In H y HRFN the strain percent and compression resistance (Rc) were measured.
32669583	8	4	theme	fique	1429:1433	arg1	matrix					1451:1456	fique lignocellulosic matrix	1429:1456	fique lignocellulosic matrix	1429:1456	The results indicate that fique lignocellulosic matrix has potential application for obtaining polymeric type composite materials.
32669583	6	5	theme	483.8 ± 283.2 nm	1184:1199	arg1	averages					1154:1161	diameter and length averages	1134:1161	diameter and length averages of 25.2 ± 6.2 nm and 483.8 ± 283.2 nm	1134:1199	The fique nanofibers showed diameter and length averages of 25.2 ± 6.2 nm and 483.8 ± 283.2 nm respectively.
32669583	7	6	theme	H	1382:1382	arg1	resistance					1368:1377	the resistance	1364:1377	the resistance of H (6.49 ± 2.48 kPa)	1364:1400	Maximum degradation temperature was 317 °C. HRFN presented higher compression resistance (16.39 ± 4.30 kPa) and this resistance was 2.5 greater than the resistance of H (6.49 ± 2.48 kPa).
32669583	1	7	theme	reinforced	243:252	arg1	hydrogels					262:270	reinforced acrylic hydrogels	243:270	reinforced acrylic hydrogels with fique nanofibres (HRFN)	243:299	Cellulose nanofibers were obtained from the Colombian fique (Furcraea bedinghausii) and Acrylic hydrogels (H) and reinforced acrylic hydrogels with fique nanofibres (HRFN) were synthesized, using the solution polymerization method.
32669583	0	8	theme	nanofibers	84:93	arg1	production					60:69	production	60:69	production of cellulose nanofibers	60:93	Valorization of Colombian fique (Furcraea bedinghausii) for production of cellulose nanofibers and its application in hydrogels.
32669583	0	8	theme	nanofibers	84:93	arg1	application					103:113	its application	99:113	its application in hydrogels	99:126	Valorization of Colombian fique (Furcraea bedinghausii) for production of cellulose nanofibers and its application in hydrogels.
32669583	1	9	theme	solution	329:336	arg1	method					353:358	the solution polymerization method	325:358	the solution polymerization method	325:358	Cellulose nanofibers were obtained from the Colombian fique (Furcraea bedinghausii) and Acrylic hydrogels (H) and reinforced acrylic hydrogels with fique nanofibres (HRFN) were synthesized, using the solution polymerization method.
32669583	4	10	theme	particle	945:952	arg1	size					954:957	the average particle size	933:957	the average particle size of the nanofibers	933:975	The surface morphology of the NAT-F and the B-F was determined by scanning electron microscopy and the average particle size of the nanofibers was made through transmission electron microscopy.
32669583	1	11	theme	acrylic	254:260	arg1	hydrogels					262:270	reinforced acrylic hydrogels	243:270	reinforced acrylic hydrogels with fique nanofibres (HRFN)	243:299	Cellulose nanofibers were obtained from the Colombian fique (Furcraea bedinghausii) and Acrylic hydrogels (H) and reinforced acrylic hydrogels with fique nanofibres (HRFN) were synthesized, using the solution polymerization method.
32669583	4	12	theme	B-F	878:880	arg1	morphology					846:855	The surface morphology	834:855	The surface morphology of the NAT-F and the B-F	834:880	The surface morphology of the NAT-F and the B-F was determined by scanning electron microscopy and the average particle size of the nanofibers was made through transmission electron microscopy.
32669583	1	13	theme	polymerization	338:351	arg1	method					353:358	the solution polymerization method	325:358	the solution polymerization method	325:358	Cellulose nanofibers were obtained from the Colombian fique (Furcraea bedinghausii) and Acrylic hydrogels (H) and reinforced acrylic hydrogels with fique nanofibres (HRFN) were synthesized, using the solution polymerization method.
32669583	2	14	dep	method	420:425	arg1	procedures					437:446	chemical procedures	428:446	chemical procedures	428:446	The extraction was carried out using a combined extraction method (chemical procedures and ultrasound radiation).
32669583	2	14	dep	method	420:425	arg1	radiation					463:471	ultrasound radiation	452:471	ultrasound radiation	452:471	The extraction was carried out using a combined extraction method (chemical procedures and ultrasound radiation).
32669583	7	15	theme	higher	1274:1279	arg1	16.39 ± 4.30 kPa					1305:1320	16.39 ± 4.30 kPa	1305:1320	16.39 ± 4.30 kPa	1305:1320	Maximum degradation temperature was 317 °C. HRFN presented higher compression resistance (16.39 ± 4.30 kPa) and this resistance was 2.5 greater than the resistance of H (6.49 ± 2.48 kPa).
32669583	7	15	theme	higher	1274:1279	arg1	resistance					1293:1302	higher compression resistance	1274:1302	higher compression resistance (16.39 ± 4.30 kPa)	1274:1321	Maximum degradation temperature was 317 °C. HRFN presented higher compression resistance (16.39 ± 4.30 kPa) and this resistance was 2.5 greater than the resistance of H (6.49 ± 2.48 kPa).
32669583	5	16	theme	H	1031:1031	arg1	HRFN					1035:1038	H y HRFN	1031:1038	H y HRFN	1031:1038	In H y HRFN the strain percent and compression resistance (Rc) were measured.
32669583	6	17	theme	25.2 ± 6.2 nm	1166:1178	arg1	averages					1154:1161	diameter and length averages	1134:1161	diameter and length averages of 25.2 ± 6.2 nm and 483.8 ± 283.2 nm	1134:1199	The fique nanofibers showed diameter and length averages of 25.2 ± 6.2 nm and 483.8 ± 283.2 nm respectively.
32669583	3	18	theme	bleached	501:508	arg1	fibers					510:515	bleached fibers	501:515	bleached fibers (B-F)	501:521	The raw material (NAT-F), bleached fibers (B-F), hydrolyzed fibers and fibers treated with ultrasound (US-F) were characterized by infrared spectroscopy (FTIR) and thermal stability analysis; also, in order to have a comparison criterion, a commercial microcrystalline cellulose sample (CC) was analyzed, which demonstrated the extraction of fique cellulose.
32669583	3	18	theme	bleached	501:508	arg1	B-F					518:520	B-F	518:520	B-F	518:520	The raw material (NAT-F), bleached fibers (B-F), hydrolyzed fibers and fibers treated with ultrasound (US-F) were characterized by infrared spectroscopy (FTIR) and thermal stability analysis; also, in order to have a comparison criterion, a commercial microcrystalline cellulose sample (CC) was analyzed, which demonstrated the extraction of fique cellulose.
32669583	3	19	theme	fique	817:821	arg1	cellulose					823:831	fique cellulose	817:831	fique cellulose	817:831	The raw material (NAT-F), bleached fibers (B-F), hydrolyzed fibers and fibers treated with ultrasound (US-F) were characterized by infrared spectroscopy (FTIR) and thermal stability analysis; also, in order to have a comparison criterion, a commercial microcrystalline cellulose sample (CC) was analyzed, which demonstrated the extraction of fique cellulose.
32669583	1	20	theme	Colombian	173:181	arg1	fique					183:187	the Colombian fique	169:187	the Colombian fique (Furcraea bedinghausii)	169:211	Cellulose nanofibers were obtained from the Colombian fique (Furcraea bedinghausii) and Acrylic hydrogels (H) and reinforced acrylic hydrogels with fique nanofibres (HRFN) were synthesized, using the solution polymerization method.
32669583	1	20	theme	Colombian	173:181	arg1	bedinghausii					199:210	Furcraea bedinghausii	190:210	Furcraea bedinghausii	190:210	Cellulose nanofibers were obtained from the Colombian fique (Furcraea bedinghausii) and Acrylic hydrogels (H) and reinforced acrylic hydrogels with fique nanofibres (HRFN) were synthesized, using the solution polymerization method.
32669583	2	21	theme	chemical	428:435	arg1	procedures					437:446	chemical procedures	428:446	chemical procedures	428:446	The extraction was carried out using a combined extraction method (chemical procedures and ultrasound radiation).
32669583	5	22	theme	y	1033:1033	arg1	HRFN					1035:1038	H y HRFN	1031:1038	H y HRFN	1031:1038	In H y HRFN the strain percent and compression resistance (Rc) were measured.
32669583	3	23	theme	cellulose	823:831	arg1	extraction					803:812	the extraction	799:812	the extraction of fique cellulose	799:831	The raw material (NAT-F), bleached fibers (B-F), hydrolyzed fibers and fibers treated with ultrasound (US-F) were characterized by infrared spectroscopy (FTIR) and thermal stability analysis; also, in order to have a comparison criterion, a commercial microcrystalline cellulose sample (CC) was analyzed, which demonstrated the extraction of fique cellulose.
32669583	4	24	theme	average	937:943	arg1	size					954:957	the average particle size	933:957	the average particle size of the nanofibers	933:975	The surface morphology of the NAT-F and the B-F was determined by scanning electron microscopy and the average particle size of the nanofibers was made through transmission electron microscopy.
32669583	8	25	theme	composite	1513:1521	arg1	materials					1523:1531	polymeric type composite materials	1498:1531	polymeric type composite materials	1498:1531	The results indicate that fique lignocellulosic matrix has potential application for obtaining polymeric type composite materials.
32669583	1	26	with	hydrogels	262:270	arg1	HRFN					295:298	HRFN	295:298	HRFN	295:298	Cellulose nanofibers were obtained from the Colombian fique (Furcraea bedinghausii) and Acrylic hydrogels (H) and reinforced acrylic hydrogels with fique nanofibres (HRFN) were synthesized, using the solution polymerization method.
32669583	1	26	with	hydrogels	262:270	arg1	nanofibres					283:292	fique nanofibres	277:292	fique nanofibres (HRFN)	277:299	Cellulose nanofibers were obtained from the Colombian fique (Furcraea bedinghausii) and Acrylic hydrogels (H) and reinforced acrylic hydrogels with fique nanofibres (HRFN) were synthesized, using the solution polymerization method.
32669583	3	27	theme	hydrolyzed	524:533	arg1	fibers					535:540	hydrolyzed fibers	524:540	hydrolyzed fibers	524:540	The raw material (NAT-F), bleached fibers (B-F), hydrolyzed fibers and fibers treated with ultrasound (US-F) were characterized by infrared spectroscopy (FTIR) and thermal stability analysis; also, in order to have a comparison criterion, a commercial microcrystalline cellulose sample (CC) was analyzed, which demonstrated the extraction of fique cellulose.
32669583	8	28	contain	has	1458:1460	arg1	matrix					1451:1456	fique lignocellulosic matrix	1429:1456	fique lignocellulosic matrix	1429:1456	The results indicate that fique lignocellulosic matrix has potential application for obtaining polymeric type composite materials.
32669583	8	28	contain	has	1458:1460	arg2	application					1472:1482	potential application	1462:1482	potential application for obtaining polymeric type composite materials	1462:1531	The results indicate that fique lignocellulosic matrix has potential application for obtaining polymeric type composite materials.
32669583	0	29	theme	fique	26:30	arg1	Valorization					0:11	Valorization	0:11	Valorization of Colombian fique (Furcraea bedinghausii) for production of cellulose nanofibers and its application in hydrogels.	0:127	Valorization of Colombian fique (Furcraea bedinghausii) for production of cellulose nanofibers and its application in hydrogels.
32669583	8	30	theme	type	1508:1511	arg1	materials					1523:1531	polymeric type composite materials	1498:1531	polymeric type composite materials	1498:1531	The results indicate that fique lignocellulosic matrix has potential application for obtaining polymeric type composite materials.
32669583	4	31	theme	scanning	900:907	arg1	microscopy					918:927	scanning electron microscopy	900:927	scanning electron microscopy	900:927	The surface morphology of the NAT-F and the B-F was determined by scanning electron microscopy and the average particle size of the nanofibers was made through transmission electron microscopy.
32669583	2	32	theme	combined	400:407	arg1	method					420:425	a combined extraction method	398:425	a combined extraction method (chemical procedures and ultrasound radiation)	398:472	The extraction was carried out using a combined extraction method (chemical procedures and ultrasound radiation).
32669583	2	33	theme	extraction	409:418	arg1	method					420:425	a combined extraction method	398:425	a combined extraction method (chemical procedures and ultrasound radiation)	398:472	The extraction was carried out using a combined extraction method (chemical procedures and ultrasound radiation).
32669583	1	34	theme	fique	277:281	arg1	HRFN					295:298	HRFN	295:298	HRFN	295:298	Cellulose nanofibers were obtained from the Colombian fique (Furcraea bedinghausii) and Acrylic hydrogels (H) and reinforced acrylic hydrogels with fique nanofibres (HRFN) were synthesized, using the solution polymerization method.
32669583	1	34	theme	fique	277:281	arg1	nanofibres					283:292	fique nanofibres	277:292	fique nanofibres (HRFN)	277:299	Cellulose nanofibers were obtained from the Colombian fique (Furcraea bedinghausii) and Acrylic hydrogels (H) and reinforced acrylic hydrogels with fique nanofibres (HRFN) were synthesized, using the solution polymerization method.
32669583	4	35	theme	surface	838:844	arg1	morphology					846:855	The surface morphology	834:855	The surface morphology of the NAT-F and the B-F	834:880	The surface morphology of the NAT-F and the B-F was determined by scanning electron microscopy and the average particle size of the nanofibers was made through transmission electron microscopy.
32669583	7	36	theme	317 °C.	1251:1257	arg1	HRFN					1259:1262	317 °C. HRFN	1251:1262	317 °C. HRFN	1251:1262	Maximum degradation temperature was 317 °C. HRFN presented higher compression resistance (16.39 ± 4.30 kPa) and this resistance was 2.5 greater than the resistance of H (6.49 ± 2.48 kPa).
32669583	0	37	theme	Furcraea	33:40	arg1	fique					26:30	Colombian fique	16:30	Colombian fique (Furcraea bedinghausii)	16:54	Valorization of Colombian fique (Furcraea bedinghausii) for production of cellulose nanofibers and its application in hydrogels.
32669583	0	37	theme	Furcraea	33:40	arg1	bedinghausii					42:53	Furcraea bedinghausii	33:53	Furcraea bedinghausii	33:53	Valorization of Colombian fique (Furcraea bedinghausii) for production of cellulose nanofibers and its application in hydrogels.
32669583	7	38	theme	Maximum	1215:1221	arg1	temperature					1235:1245	Maximum degradation temperature	1215:1245	Maximum degradation temperature	1215:1245	Maximum degradation temperature was 317 °C. HRFN presented higher compression resistance (16.39 ± 4.30 kPa) and this resistance was 2.5 greater than the resistance of H (6.49 ± 2.48 kPa).
32669583	4	39	theme	NAT-F	864:868	arg1	morphology					846:855	The surface morphology	834:855	The surface morphology of the NAT-F and the B-F	834:880	The surface morphology of the NAT-F and the B-F was determined by scanning electron microscopy and the average particle size of the nanofibers was made through transmission electron microscopy.
32669583	6	40	theme	length	1147:1152	arg1	averages					1154:1161	diameter and length averages	1134:1161	diameter and length averages of 25.2 ± 6.2 nm and 483.8 ± 283.2 nm	1134:1199	The fique nanofibers showed diameter and length averages of 25.2 ± 6.2 nm and 483.8 ± 283.2 nm respectively.
32669583	7	41	theme	degradation	1223:1233	arg1	temperature					1235:1245	Maximum degradation temperature	1215:1245	Maximum degradation temperature	1215:1245	Maximum degradation temperature was 317 °C. HRFN presented higher compression resistance (16.39 ± 4.30 kPa) and this resistance was 2.5 greater than the resistance of H (6.49 ± 2.48 kPa).
32669583	2	42	theme	ultrasound	452:461	arg1	radiation					463:471	ultrasound radiation	452:471	ultrasound radiation	452:471	The extraction was carried out using a combined extraction method (chemical procedures and ultrasound radiation).
32669583	4	43	theme	electron	909:916	arg1	microscopy					918:927	scanning electron microscopy	900:927	scanning electron microscopy	900:927	The surface morphology of the NAT-F and the B-F was determined by scanning electron microscopy and the average particle size of the nanofibers was made through transmission electron microscopy.
32669583	0	44	from	production	60:69	arg1	hydrogels					118:126	hydrogels	118:126	hydrogels	118:126	Valorization of Colombian fique (Furcraea bedinghausii) for production of cellulose nanofibers and its application in hydrogels.
32669583	7	45	dep	presented	1264:1272	arg1	HRFN					1259:1262	317 °C. HRFN	1251:1262	317 °C. HRFN	1251:1262	Maximum degradation temperature was 317 °C. HRFN presented higher compression resistance (16.39 ± 4.30 kPa) and this resistance was 2.5 greater than the resistance of H (6.49 ± 2.48 kPa).
32669583	5	46	theme	compression	1063:1073	arg1	resistance					1075:1084	compression resistance	1063:1084	compression resistance (Rc)	1063:1089	In H y HRFN the strain percent and compression resistance (Rc) were measured.
32669583	5	46	theme	compression	1063:1073	arg1	Rc					1087:1088	Rc	1087:1088	Rc	1087:1088	In H y HRFN the strain percent and compression resistance (Rc) were measured.
32669583	6	47	theme	diameter	1134:1141	arg1	averages					1154:1161	diameter and length averages	1134:1161	diameter and length averages of 25.2 ± 6.2 nm and 483.8 ± 283.2 nm	1134:1199	The fique nanofibers showed diameter and length averages of 25.2 ± 6.2 nm and 483.8 ± 283.2 nm respectively.
32669583	8	48	theme	polymeric	1498:1506	arg1	materials					1523:1531	polymeric type composite materials	1498:1531	polymeric type composite materials	1498:1531	The results indicate that fique lignocellulosic matrix has potential application for obtaining polymeric type composite materials.
32669583	0	49	from	application	103:113	arg1	hydrogels					118:126	hydrogels	118:126	hydrogels	118:126	Valorization of Colombian fique (Furcraea bedinghausii) for production of cellulose nanofibers and its application in hydrogels.
32669583	7	50	theme	compression	1281:1291	arg1	16.39 ± 4.30 kPa					1305:1320	16.39 ± 4.30 kPa	1305:1320	16.39 ± 4.30 kPa	1305:1320	Maximum degradation temperature was 317 °C. HRFN presented higher compression resistance (16.39 ± 4.30 kPa) and this resistance was 2.5 greater than the resistance of H (6.49 ± 2.48 kPa).
32669583	7	50	theme	compression	1281:1291	arg1	resistance					1293:1302	higher compression resistance	1274:1302	higher compression resistance (16.39 ± 4.30 kPa)	1274:1321	Maximum degradation temperature was 317 °C. HRFN presented higher compression resistance (16.39 ± 4.30 kPa) and this resistance was 2.5 greater than the resistance of H (6.49 ± 2.48 kPa).
32669583	3	51	theme	raw	479:481	arg1	NAT-F					493:497	NAT-F	493:497	NAT-F	493:497	The raw material (NAT-F), bleached fibers (B-F), hydrolyzed fibers and fibers treated with ultrasound (US-F) were characterized by infrared spectroscopy (FTIR) and thermal stability analysis; also, in order to have a comparison criterion, a commercial microcrystalline cellulose sample (CC) was analyzed, which demonstrated the extraction of fique cellulose.
32669583	3	51	theme	raw	479:481	arg1	material					483:490	The raw material	475:490	The raw material (NAT-F)	475:498	The raw material (NAT-F), bleached fibers (B-F), hydrolyzed fibers and fibers treated with ultrasound (US-F) were characterized by infrared spectroscopy (FTIR) and thermal stability analysis; also, in order to have a comparison criterion, a commercial microcrystalline cellulose sample (CC) was analyzed, which demonstrated the extraction of fique cellulose.
32669583	4	52	theme	transmission	994:1005	arg1	microscopy					1016:1025	transmission electron microscopy	994:1025	transmission electron microscopy	994:1025	The surface morphology of the NAT-F and the B-F was determined by scanning electron microscopy and the average particle size of the nanofibers was made through transmission electron microscopy.
32669583	3	53	theme	comparison	692:701	arg1	criterion					703:711	a comparison criterion	690:711	a comparison criterion	690:711	The raw material (NAT-F), bleached fibers (B-F), hydrolyzed fibers and fibers treated with ultrasound (US-F) were characterized by infrared spectroscopy (FTIR) and thermal stability analysis; also, in order to have a comparison criterion, a commercial microcrystalline cellulose sample (CC) was analyzed, which demonstrated the extraction of fique cellulose.
32669583	1	54	theme	Acrylic	217:223	arg1	H					236:236	H	236:236	H	236:236	Cellulose nanofibers were obtained from the Colombian fique (Furcraea bedinghausii) and Acrylic hydrogels (H) and reinforced acrylic hydrogels with fique nanofibres (HRFN) were synthesized, using the solution polymerization method.
32669583	1	54	theme	Acrylic	217:223	arg1	hydrogels					225:233	Acrylic hydrogels	217:233	Acrylic hydrogels (H)	217:237	Cellulose nanofibers were obtained from the Colombian fique (Furcraea bedinghausii) and Acrylic hydrogels (H) and reinforced acrylic hydrogels with fique nanofibres (HRFN) were synthesized, using the solution polymerization method.
32669583	3	55	theme	infrared	606:613	arg1	spectroscopy					615:626	infrared spectroscopy	606:626	infrared spectroscopy (FTIR)	606:633	The raw material (NAT-F), bleached fibers (B-F), hydrolyzed fibers and fibers treated with ultrasound (US-F) were characterized by infrared spectroscopy (FTIR) and thermal stability analysis; also, in order to have a comparison criterion, a commercial microcrystalline cellulose sample (CC) was analyzed, which demonstrated the extraction of fique cellulose.
32669583	3	55	theme	infrared	606:613	arg1	FTIR					629:632	FTIR	629:632	FTIR	629:632	The raw material (NAT-F), bleached fibers (B-F), hydrolyzed fibers and fibers treated with ultrasound (US-F) were characterized by infrared spectroscopy (FTIR) and thermal stability analysis; also, in order to have a comparison criterion, a commercial microcrystalline cellulose sample (CC) was analyzed, which demonstrated the extraction of fique cellulose.
32669583	6	56	theme	fique	1110:1114	arg1	nanofibers					1116:1125	The fique nanofibers	1106:1125	The fique nanofibers	1106:1125	The fique nanofibers showed diameter and length averages of 25.2 ± 6.2 nm and 483.8 ± 283.2 nm respectively.
32669583	8	57	theme	lignocellulosic	1435:1449	arg1	matrix					1451:1456	fique lignocellulosic matrix	1429:1456	fique lignocellulosic matrix	1429:1456	The results indicate that fique lignocellulosic matrix has potential application for obtaining polymeric type composite materials.
32669583	1	58	theme	Cellulose	129:137	arg1	nanofibers					139:148	Cellulose nanofibers	129:148	Cellulose nanofibers	129:148	Cellulose nanofibers were obtained from the Colombian fique (Furcraea bedinghausii) and Acrylic hydrogels (H) and reinforced acrylic hydrogels with fique nanofibres (HRFN) were synthesized, using the solution polymerization method.
32669583	3	59	theme	cellulose	744:752	arg1	CC					762:763	CC	762:763	CC	762:763	The raw material (NAT-F), bleached fibers (B-F), hydrolyzed fibers and fibers treated with ultrasound (US-F) were characterized by infrared spectroscopy (FTIR) and thermal stability analysis; also, in order to have a comparison criterion, a commercial microcrystalline cellulose sample (CC) was analyzed, which demonstrated the extraction of fique cellulose.
32669583	3	59	theme	cellulose	744:752	arg1	sample					754:759	a commercial microcrystalline cellulose sample	714:759	a commercial microcrystalline cellulose sample (CC)	714:764	The raw material (NAT-F), bleached fibers (B-F), hydrolyzed fibers and fibers treated with ultrasound (US-F) were characterized by infrared spectroscopy (FTIR) and thermal stability analysis; also, in order to have a comparison criterion, a commercial microcrystalline cellulose sample (CC) was analyzed, which demonstrated the extraction of fique cellulose.
32669583	8	60	theme	potential	1462:1470	arg1	application					1472:1482	potential application	1462:1482	potential application for obtaining polymeric type composite materials	1462:1531	The results indicate that fique lignocellulosic matrix has potential application for obtaining polymeric type composite materials.
32669583	1	61	theme	Furcraea	190:197	arg1	fique					183:187	the Colombian fique	169:187	the Colombian fique (Furcraea bedinghausii)	169:211	Cellulose nanofibers were obtained from the Colombian fique (Furcraea bedinghausii) and Acrylic hydrogels (H) and reinforced acrylic hydrogels with fique nanofibres (HRFN) were synthesized, using the solution polymerization method.
32669583	1	61	theme	Furcraea	190:197	arg1	bedinghausii					199:210	Furcraea bedinghausii	190:210	Furcraea bedinghausii	190:210	Cellulose nanofibers were obtained from the Colombian fique (Furcraea bedinghausii) and Acrylic hydrogels (H) and reinforced acrylic hydrogels with fique nanofibres (HRFN) were synthesized, using the solution polymerization method.
32669583	4	62	theme	electron	1007:1014	arg1	microscopy					1016:1025	transmission electron microscopy	994:1025	transmission electron microscopy	994:1025	The surface morphology of the NAT-F and the B-F was determined by scanning electron microscopy and the average particle size of the nanofibers was made through transmission electron microscopy.
32669583	3	63	theme	thermal	639:645	arg1	analysis					657:664	thermal stability analysis	639:664	thermal stability analysis	639:664	The raw material (NAT-F), bleached fibers (B-F), hydrolyzed fibers and fibers treated with ultrasound (US-F) were characterized by infrared spectroscopy (FTIR) and thermal stability analysis; also, in order to have a comparison criterion, a commercial microcrystalline cellulose sample (CC) was analyzed, which demonstrated the extraction of fique cellulose.
32669583	0	64	theme	Colombian	16:24	arg1	fique					26:30	Colombian fique	16:30	Colombian fique (Furcraea bedinghausii)	16:54	Valorization of Colombian fique (Furcraea bedinghausii) for production of cellulose nanofibers and its application in hydrogels.
32669583	0	64	theme	Colombian	16:24	arg1	bedinghausii					42:53	Furcraea bedinghausii	33:53	Furcraea bedinghausii	33:53	Valorization of Colombian fique (Furcraea bedinghausii) for production of cellulose nanofibers and its application in hydrogels.
32669583	3	65	theme	microcrystalline	727:742	arg1	cellulose					744:752	a commercial microcrystalline cellulose	714:752	a commercial microcrystalline cellulose sample (CC)	714:764	The raw material (NAT-F), bleached fibers (B-F), hydrolyzed fibers and fibers treated with ultrasound (US-F) were characterized by infrared spectroscopy (FTIR) and thermal stability analysis; also, in order to have a comparison criterion, a commercial microcrystalline cellulose sample (CC) was analyzed, which demonstrated the extraction of fique cellulose.
32669583	4	66	theme	nanofibers	966:975	arg1	size					954:957	the average particle size	933:957	the average particle size of the nanofibers	933:975	The surface morphology of the NAT-F and the B-F was determined by scanning electron microscopy and the average particle size of the nanofibers was made through transmission electron microscopy.
32669583	3	67	theme	commercial	716:725	arg1	cellulose					744:752	a commercial microcrystalline cellulose	714:752	a commercial microcrystalline cellulose sample (CC)	714:764	The raw material (NAT-F), bleached fibers (B-F), hydrolyzed fibers and fibers treated with ultrasound (US-F) were characterized by infrared spectroscopy (FTIR) and thermal stability analysis; also, in order to have a comparison criterion, a commercial microcrystalline cellulose sample (CC) was analyzed, which demonstrated the extraction of fique cellulose.
32989628	10	0	theme	mixed-breed	1283:1293	arg1	goats					1295:1299	mixed-breed goats	1283:1299	mixed-breed goats	1283:1299	In conclusion, moderate milk yield of mixed-breed goats on rangeland is feasible with parturitions in winter, and milk yield is not affected by reduced annual precipitation in rangelands of northern Mexico.
32989628	3	1	theme	Body	442:445	arg1	highest					484:490	highest	484:490	highest	484:490	Body weight loss during lactation was the highest (6.4 kg) for goats on rangeland with 441 mm of rainfall than goats on 221 mm (1.6 kg), or 334 mm (1.8 kg; P < 0.01).
32989628	3	1	theme	Body	442:445	arg1	loss					454:457	Body weight loss	442:457	Body weight loss during lactation	442:474	Body weight loss during lactation was the highest (6.4 kg) for goats on rangeland with 441 mm of rainfall than goats on 221 mm (1.6 kg), or 334 mm (1.8 kg; P < 0.01).
32989628	3	2	from	goats	553:557	arg1	1.6 kg					570:575	1.6 kg	570:575	1.6 kg	570:575	Body weight loss during lactation was the highest (6.4 kg) for goats on rangeland with 441 mm of rainfall than goats on 221 mm (1.6 kg), or 334 mm (1.8 kg; P < 0.01).
32989628	3	2	from	goats	553:557	arg1	221 mm					562:567	221 mm	562:567	221 mm (1.6 kg)	562:576	Body weight loss during lactation was the highest (6.4 kg) for goats on rangeland with 441 mm of rainfall than goats on 221 mm (1.6 kg), or 334 mm (1.8 kg; P < 0.01).
32989628	3	2	from	goats	553:557	arg1	334 mm					582:587	334 mm	582:587	334 mm (1.8 kg; P < 0.01)	582:606	Body weight loss during lactation was the highest (6.4 kg) for goats on rangeland with 441 mm of rainfall than goats on 221 mm (1.6 kg), or 334 mm (1.8 kg; P < 0.01).
32989628	7	3	theme	Milk	919:922	arg1	protein					924:930	Milk protein and lactose content	919:950	protein	924:930	Milk protein and lactose content were higher in goats on rangeland with 221 mm (P < 0.01).
32989628	5	4	theme	lactation	715:723	arg1	peak					707:710	an evident peak	696:710	an evident peak of lactation	696:723	There was not an evident peak of lactation and milk yield increased uninterruptedly at the end of lactation.
32989628	0	5	theme	different	107:115	arg1	rainfall					117:124	different rainfall	107:124	different rainfall	107:124	Milk yield and composition and body weight of offsprings of mixed-breed goats on semi-arid rangelands with different rainfall.
32989628	1	6	theme	rainfall	314:321	arg1	mm					301:302	221 (n = 15), 334 (n = 20), or 441 (n = 12) mm	257:302	221 (n = 15), 334 (n = 20), or 441 (n = 12) mm of annual rainfall in northern Mexico (22-25°N)	257:350	The objective was to characterize milk yield and composition from mixed-breed goats kidding in winter and grazing rangelands with 221 (n = 15), 334 (n = 20), or 441 (n = 12) mm of annual rainfall in northern Mexico (22-25°N).
32989628	1	7	theme	mixed-breed	193:203	arg1	goats					205:209	mixed-breed goats	193:209	mixed-breed goats kidding in winter and grazing rangelands with 221 (n = 15), 334 (n = 20), or 441 (n = 12) mm of annual rainfall in northern Mexico (22-25°N)	193:350	The objective was to characterize milk yield and composition from mixed-breed goats kidding in winter and grazing rangelands with 221 (n = 15), 334 (n = 20), or 441 (n = 12) mm of annual rainfall in northern Mexico (22-25°N).
32989628	1	8	theme	northern	326:333	arg1	22-25°N					343:349	22-25°N	343:349	22-25°N	343:349	The objective was to characterize milk yield and composition from mixed-breed goats kidding in winter and grazing rangelands with 221 (n = 15), 334 (n = 20), or 441 (n = 12) mm of annual rainfall in northern Mexico (22-25°N).
32989628	1	8	theme	northern	326:333	arg1	Mexico					335:340	northern Mexico	326:340	northern Mexico (22-25°N)	326:350	The objective was to characterize milk yield and composition from mixed-breed goats kidding in winter and grazing rangelands with 221 (n = 15), 334 (n = 20), or 441 (n = 12) mm of annual rainfall in northern Mexico (22-25°N).
32989628	1	9	from	goats	205:209	arg1	composition					176:186	composition	176:186	composition	176:186	The objective was to characterize milk yield and composition from mixed-breed goats kidding in winter and grazing rangelands with 221 (n = 15), 334 (n = 20), or 441 (n = 12) mm of annual rainfall in northern Mexico (22-25°N).
32989628	1	9	from	goats	205:209	arg1	yield					166:170	milk yield	161:170	milk yield	161:170	The objective was to characterize milk yield and composition from mixed-breed goats kidding in winter and grazing rangelands with 221 (n = 15), 334 (n = 20), or 441 (n = 12) mm of annual rainfall in northern Mexico (22-25°N).
32989628	0	10	from	offsprings	46:55	arg1	rangelands					91:100	semi-arid rangelands	81:100	semi-arid rangelands with different rainfall	81:124	Milk yield and composition and body weight of offsprings of mixed-breed goats on semi-arid rangelands with different rainfall.
32989628	10	11	theme	milk	1359:1362	arg1	yield					1364:1368	milk yield	1359:1368	milk yield	1359:1368	In conclusion, moderate milk yield of mixed-breed goats on rangeland is feasible with parturitions in winter, and milk yield is not affected by reduced annual precipitation in rangelands of northern Mexico.
32989628	0	12	from	rangelands	91:100	arg1	offsprings					46:55	offsprings	46:55	offsprings of mixed-breed goats on semi-arid rangelands with different rainfall	46:124	Milk yield and composition and body weight of offsprings of mixed-breed goats on semi-arid rangelands with different rainfall.
32989628	3	13	theme	rainfall	539:546	arg1	441 mm					529:534	441 mm	529:534	441 mm of rainfall than goats on 221 mm (1.6 kg), or 334 mm (1.8 kg; P < 0.01)	529:606	Body weight loss during lactation was the highest (6.4 kg) for goats on rangeland with 441 mm of rainfall than goats on 221 mm (1.6 kg), or 334 mm (1.8 kg; P < 0.01).
32989628	11	14	theme	maternal	1492:1499	arg1	yield					1506:1510	maternal milk yield	1492:1510	maternal milk yield	1492:1510	Offspring growth performance depends on maternal milk yield, but not milk composition.
32989628	0	15	theme	offsprings	46:55	arg1	composition					15:25	composition	15:25	composition	15:25	Milk yield and composition and body weight of offsprings of mixed-breed goats on semi-arid rangelands with different rainfall.
32989628	0	15	theme	offsprings	46:55	arg1	weight					36:41	body weight	31:41	body weight	31:41	Milk yield and composition and body weight of offsprings of mixed-breed goats on semi-arid rangelands with different rainfall.
32989628	5	16	theme	milk	729:732	arg1	yield					734:738	milk yield	729:738	milk yield	729:738	There was not an evident peak of lactation and milk yield increased uninterruptedly at the end of lactation.
32989628	10	17	from	conclusion	1248:1257	arg1	feasible					1317:1324	feasible	1317:1324	feasible	1317:1324	In conclusion, moderate milk yield of mixed-breed goats on rangeland is feasible with parturitions in winter, and milk yield is not affected by reduced annual precipitation in rangelands of northern Mexico.
32989628	2	18	theme	growth	413:418	arg1	performance					420:430	progeny growth performance	405:430	progeny growth performance	405:430	Weekly milk yield and composition were recorded and progeny growth performance assessed.
32989628	11	19	theme	milk	1521:1524	arg1	composition					1526:1536	milk composition	1521:1536	milk composition	1521:1536	Offspring growth performance depends on maternal milk yield, but not milk composition.
32989628	0	20	theme	mixed-breed	60:70	arg1	goats					72:76	mixed-breed goats	60:76	mixed-breed goats on semi-arid rangelands with different rainfall	60:124	Milk yield and composition and body weight of offsprings of mixed-breed goats on semi-arid rangelands with different rainfall.
32989628	4	21	from	goats	646:650	arg1	221 mm					655:660	221 mm	655:660	221 mm (498 mL; P < 0.01)	655:679	The highest daily milk yield was for goats on 221 mm (498 mL; P < 0.01).
32989628	7	22	from	goats	967:971	arg1	rangeland					976:984	rangeland	976:984	rangeland with 221 mm (P < 0.01)	976:1007	Milk protein and lactose content were higher in goats on rangeland with 221 mm (P < 0.01).
32989628	1	23	from	Mexico	335:340	arg1	mm					301:302	221 (n = 15), 334 (n = 20), or 441 (n = 12) mm	257:302	221 (n = 15), 334 (n = 20), or 441 (n = 12) mm of annual rainfall in northern Mexico (22-25°N)	257:350	The objective was to characterize milk yield and composition from mixed-breed goats kidding in winter and grazing rangelands with 221 (n = 15), 334 (n = 20), or 441 (n = 12) mm of annual rainfall in northern Mexico (22-25°N).
32989628	10	24	from	yield	1274:1278	arg1	rangeland					1304:1312	rangeland	1304:1312	rangeland	1304:1312	In conclusion, moderate milk yield of mixed-breed goats on rangeland is feasible with parturitions in winter, and milk yield is not affected by reduced annual precipitation in rangelands of northern Mexico.
32989628	6	25	contain	had	872:874	arg2	P < 0.001					907:915	P < 0.001	907:915	P < 0.001	907:915	Milk fat was negatively related to milk yield and goats on rangeland with 334 mm had the greatest milk fat content (P < 0.001).
32989628	6	25	contain	had	872:874	arg2	content					898:904	milk fat content	889:904	the greatest milk fat content (P < 0.001)	876:916	Milk fat was negatively related to milk yield and goats on rangeland with 334 mm had the greatest milk fat content (P < 0.001).
32989628	6	25	contain	had	872:874	arg1	goats					841:845	goats	841:845	goats on rangeland with 334 mm	841:870	Milk fat was negatively related to milk yield and goats on rangeland with 334 mm had the greatest milk fat content (P < 0.001).
32989628	10	26	theme	annual	1397:1402	arg1	precipitation					1404:1416	reduced annual precipitation	1389:1416	reduced annual precipitation in rangelands of northern Mexico	1389:1449	In conclusion, moderate milk yield of mixed-breed goats on rangeland is feasible with parturitions in winter, and milk yield is not affected by reduced annual precipitation in rangelands of northern Mexico.
32989628	3	27	with	rangeland	514:522	arg1	441 mm					529:534	441 mm	529:534	441 mm of rainfall than goats on 221 mm (1.6 kg), or 334 mm (1.8 kg; P < 0.01)	529:606	Body weight loss during lactation was the highest (6.4 kg) for goats on rangeland with 441 mm of rainfall than goats on 221 mm (1.6 kg), or 334 mm (1.8 kg; P < 0.01).
32989628	12	28	theme	adequate	1626:1633	arg1	weight					1642:1647	adequate market weight	1626:1647	adequate market weight	1626:1647	Nevertheless, slow growth to weaning of offspring had adverse effects on time to reach adequate market weight.
32989628	4	29	theme	highest	613:619	arg1	yield					632:636	The highest daily milk yield	609:636	The highest daily milk yield	609:636	The highest daily milk yield was for goats on 221 mm (498 mL; P < 0.01).
32989628	1	30	theme	n = 15	262:267	arg1	mm					301:302	221 (n = 15), 334 (n = 20), or 441 (n = 12) mm	257:302	221 (n = 15), 334 (n = 20), or 441 (n = 12) mm of annual rainfall in northern Mexico (22-25°N)	257:350	The objective was to characterize milk yield and composition from mixed-breed goats kidding in winter and grazing rangelands with 221 (n = 15), 334 (n = 20), or 441 (n = 12) mm of annual rainfall in northern Mexico (22-25°N).
32989628	5	31	theme	lactation	780:788	arg1	end					773:775	the end	769:775	the end of lactation	769:788	There was not an evident peak of lactation and milk yield increased uninterruptedly at the end of lactation.
32989628	6	32	theme	milk	889:892	arg1	P < 0.001					907:915	P < 0.001	907:915	P < 0.001	907:915	Milk fat was negatively related to milk yield and goats on rangeland with 334 mm had the greatest milk fat content (P < 0.001).
32989628	6	32	theme	milk	889:892	arg1	content					898:904	milk fat content	889:904	the greatest milk fat content (P < 0.001)	876:916	Milk fat was negatively related to milk yield and goats on rangeland with 334 mm had the greatest milk fat content (P < 0.001).
32989628	2	33	theme	milk	360:363	arg1	yield					365:369	Weekly milk yield	353:369	Weekly milk yield	353:369	Weekly milk yield and composition were recorded and progeny growth performance assessed.
32989628	4	34	theme	milk	627:630	arg1	yield					632:636	The highest daily milk yield	609:636	The highest daily milk yield	609:636	The highest daily milk yield was for goats on 221 mm (498 mL; P < 0.01).
32989628	1	35	from	mm	301:302	arg1	22-25°N					343:349	22-25°N	343:349	22-25°N	343:349	The objective was to characterize milk yield and composition from mixed-breed goats kidding in winter and grazing rangelands with 221 (n = 15), 334 (n = 20), or 441 (n = 12) mm of annual rainfall in northern Mexico (22-25°N).
32989628	1	35	from	mm	301:302	arg1	Mexico					335:340	northern Mexico	326:340	northern Mexico (22-25°N)	326:350	The objective was to characterize milk yield and composition from mixed-breed goats kidding in winter and grazing rangelands with 221 (n = 15), 334 (n = 20), or 441 (n = 12) mm of annual rainfall in northern Mexico (22-25°N).
32989628	11	36	theme	Offspring	1452:1460	arg1	performance					1469:1479	Offspring growth performance	1452:1479	Offspring growth performance	1452:1479	Offspring growth performance depends on maternal milk yield, but not milk composition.
32989628	1	37	theme	milk	161:164	arg1	yield					166:170	milk yield	161:170	milk yield	161:170	The objective was to characterize milk yield and composition from mixed-breed goats kidding in winter and grazing rangelands with 221 (n = 15), 334 (n = 20), or 441 (n = 12) mm of annual rainfall in northern Mexico (22-25°N).
32989628	12	38	theme	offspring	1579:1587	arg1	growth					1558:1563	slow growth	1553:1563	slow growth to weaning of offspring	1553:1587	Nevertheless, slow growth to weaning of offspring had adverse effects on time to reach adequate market weight.
32989628	10	39	from	feasible	1317:1324	arg1	conclusion					1248:1257	conclusion	1248:1257	conclusion	1248:1257	In conclusion, moderate milk yield of mixed-breed goats on rangeland is feasible with parturitions in winter, and milk yield is not affected by reduced annual precipitation in rangelands of northern Mexico.
32989628	1	40	with	rangelands	241:250	arg1	mm					301:302	221 (n = 15), 334 (n = 20), or 441 (n = 12) mm	257:302	221 (n = 15), 334 (n = 20), or 441 (n = 12) mm of annual rainfall in northern Mexico (22-25°N)	257:350	The objective was to characterize milk yield and composition from mixed-breed goats kidding in winter and grazing rangelands with 221 (n = 15), 334 (n = 20), or 441 (n = 12) mm of annual rainfall in northern Mexico (22-25°N).
32989628	10	41	theme	milk	1269:1272	arg1	yield					1274:1278	moderate milk yield	1260:1278	moderate milk yield of mixed-breed goats on rangeland	1260:1312	In conclusion, moderate milk yield of mixed-breed goats on rangeland is feasible with parturitions in winter, and milk yield is not affected by reduced annual precipitation in rangelands of northern Mexico.
32989628	10	42	theme	Mexico	1444:1449	arg1	rangelands					1421:1430	rangelands	1421:1430	rangelands of northern Mexico	1421:1449	In conclusion, moderate milk yield of mixed-breed goats on rangeland is feasible with parturitions in winter, and milk yield is not affected by reduced annual precipitation in rangelands of northern Mexico.
32989628	12	43	theme	adverse	1593:1599	arg1	effects					1601:1607	adverse effects	1593:1607	adverse effects	1593:1607	Nevertheless, slow growth to weaning of offspring had adverse effects on time to reach adequate market weight.
32989628	10	44	from	parturitions	1331:1342	arg1	winter					1347:1352	winter	1347:1352	winter	1347:1352	In conclusion, moderate milk yield of mixed-breed goats on rangeland is feasible with parturitions in winter, and milk yield is not affected by reduced annual precipitation in rangelands of northern Mexico.
32989628	3	45	theme	weight	447:452	arg1	highest					484:490	highest	484:490	highest	484:490	Body weight loss during lactation was the highest (6.4 kg) for goats on rangeland with 441 mm of rainfall than goats on 221 mm (1.6 kg), or 334 mm (1.8 kg; P < 0.01).
32989628	3	45	theme	weight	447:452	arg1	loss					454:457	Body weight loss	442:457	Body weight loss during lactation	442:474	Body weight loss during lactation was the highest (6.4 kg) for goats on rangeland with 441 mm of rainfall than goats on 221 mm (1.6 kg), or 334 mm (1.8 kg; P < 0.01).
32989628	8	46	dep	heavier	1075:1081	arg1	P < 0.001					1084:1092	P < 0.001	1084:1092	P < 0.001	1084:1092	Goat kids on rangelands with 221 and 441 mm grew faster and were heavier (P < 0.001) at weaning than kids reared in other rangelands.
32989628	12	47	theme	slow	1553:1556	arg1	growth					1558:1563	slow growth	1553:1563	slow growth to weaning of offspring	1553:1587	Nevertheless, slow growth to weaning of offspring had adverse effects on time to reach adequate market weight.
32989628	1	48	theme	n = 20	276:281	arg1	mm					301:302	221 (n = 15), 334 (n = 20), or 441 (n = 12) mm	257:302	221 (n = 15), 334 (n = 20), or 441 (n = 12) mm of annual rainfall in northern Mexico (22-25°N)	257:350	The objective was to characterize milk yield and composition from mixed-breed goats kidding in winter and grazing rangelands with 221 (n = 15), 334 (n = 20), or 441 (n = 12) mm of annual rainfall in northern Mexico (22-25°N).
32989628	10	49	theme	goats	1295:1299	arg1	yield					1274:1278	moderate milk yield	1260:1278	moderate milk yield of mixed-breed goats on rangeland	1260:1312	In conclusion, moderate milk yield of mixed-breed goats on rangeland is feasible with parturitions in winter, and milk yield is not affected by reduced annual precipitation in rangelands of northern Mexico.
32989628	7	50	theme	lactose	936:942	arg1	content					944:950	Milk protein and lactose content	919:950	content	944:950	Milk protein and lactose content were higher in goats on rangeland with 221 mm (P < 0.01).
32989628	5	51	theme	evident	699:705	arg1	peak					707:710	an evident peak	696:710	an evident peak of lactation	696:723	There was not an evident peak of lactation and milk yield increased uninterruptedly at the end of lactation.
32989628	8	52	from	kids	1015:1018	arg1	rangelands					1023:1032	rangelands	1023:1032	rangelands with 221 and 441 mm	1023:1052	Goat kids on rangelands with 221 and 441 mm grew faster and were heavier (P < 0.001) at weaning than kids reared in other rangelands.
32989628	10	53	with	feasible	1317:1324	arg1	parturitions					1331:1342	parturitions	1331:1342	parturitions in winter	1331:1352	In conclusion, moderate milk yield of mixed-breed goats on rangeland is feasible with parturitions in winter, and milk yield is not affected by reduced annual precipitation in rangelands of northern Mexico.
32989628	0	54	with	rangelands	91:100	arg1	rainfall					117:124	different rainfall	107:124	different rainfall	107:124	Milk yield and composition and body weight of offsprings of mixed-breed goats on semi-arid rangelands with different rainfall.
32989628	4	55	dep	221 mm	655:660	arg1	498 mL					663:668	498 mL	663:668	498 mL; P < 0.01	663:678	The highest daily milk yield was for goats on 221 mm (498 mL; P < 0.01).
32989628	7	56	with	rangeland	976:984	arg1	P < 0.01					999:1006	P < 0.01	999:1006	P < 0.01	999:1006	Milk protein and lactose content were higher in goats on rangeland with 221 mm (P < 0.01).
32989628	7	56	with	rangeland	976:984	arg1	221 mm					991:996	221 mm	991:996	221 mm (P < 0.01)	991:1007	Milk protein and lactose content were higher in goats on rangeland with 221 mm (P < 0.01).
32989628	0	57	from	goats	72:76	arg1	rangelands					91:100	semi-arid rangelands	81:100	semi-arid rangelands with different rainfall	81:124	Milk yield and composition and body weight of offsprings of mixed-breed goats on semi-arid rangelands with different rainfall.
32989628	9	58	theme	milk	1217:1220	arg1	P > 0.05					1234:1241	P > 0.05	1234:1241	P > 0.05	1234:1241	Weaning weight was positively related to milk yield (P < 0.001); but not milk components (P > 0.05).
32989628	9	58	theme	milk	1217:1220	arg1	components					1222:1231	milk components	1217:1231	milk yield (P < 0.001); but not milk components (P > 0.05)	1185:1242	Weaning weight was positively related to milk yield (P < 0.001); but not milk components (P > 0.05).
32989628	8	59	theme	Goat	1010:1013	arg1	kids					1015:1018	Goat kids	1010:1018	Goat kids on rangelands with 221 and 441 mm	1010:1052	Goat kids on rangelands with 221 and 441 mm grew faster and were heavier (P < 0.001) at weaning than kids reared in other rangelands.
32989628	6	60	theme	Milk	791:794	arg1	fat					796:798	Milk fat	791:798	Milk fat	791:798	Milk fat was negatively related to milk yield and goats on rangeland with 334 mm had the greatest milk fat content (P < 0.001).
32989628	6	61	with	goats	841:845	arg1	334 mm					865:870	334 mm	865:870	334 mm	865:870	Milk fat was negatively related to milk yield and goats on rangeland with 334 mm had the greatest milk fat content (P < 0.001).
32989628	8	62	with	rangelands	1023:1032	arg1	441 mm					1047:1052	441 mm	1047:1052	441 mm	1047:1052	Goat kids on rangelands with 221 and 441 mm grew faster and were heavier (P < 0.001) at weaning than kids reared in other rangelands.
32989628	8	62	with	rangelands	1023:1032	arg1	221					1039:1041	221	1039:1041	221	1039:1041	Goat kids on rangelands with 221 and 441 mm grew faster and were heavier (P < 0.001) at weaning than kids reared in other rangelands.
32989628	11	63	theme	milk	1501:1504	arg1	yield					1506:1510	maternal milk yield	1492:1510	maternal milk yield	1492:1510	Offspring growth performance depends on maternal milk yield, but not milk composition.
32989628	0	64	theme	goats	72:76	arg1	offsprings					46:55	offsprings	46:55	offsprings of mixed-breed goats on semi-arid rangelands with different rainfall	46:124	Milk yield and composition and body weight of offsprings of mixed-breed goats on semi-arid rangelands with different rainfall.
32989628	1	65	theme	441	288:290	arg1	mm					301:302	221 (n = 15), 334 (n = 20), or 441 (n = 12) mm	257:302	221 (n = 15), 334 (n = 20), or 441 (n = 12) mm of annual rainfall in northern Mexico (22-25°N)	257:350	The objective was to characterize milk yield and composition from mixed-breed goats kidding in winter and grazing rangelands with 221 (n = 15), 334 (n = 20), or 441 (n = 12) mm of annual rainfall in northern Mexico (22-25°N).
32989628	2	66	theme	progeny	405:411	arg1	performance					420:430	progeny growth performance	405:430	progeny growth performance	405:430	Weekly milk yield and composition were recorded and progeny growth performance assessed.
32989628	6	67	from	goats	841:845	arg1	rangeland					850:858	rangeland	850:858	rangeland	850:858	Milk fat was negatively related to milk yield and goats on rangeland with 334 mm had the greatest milk fat content (P < 0.001).
32989628	0	68	theme	semi-arid	81:89	arg1	rangelands					91:100	semi-arid rangelands	81:100	semi-arid rangelands with different rainfall	81:124	Milk yield and composition and body weight of offsprings of mixed-breed goats on semi-arid rangelands with different rainfall.
32989628	6	69	theme	milk	826:829	arg1	yield					831:835	milk yield	826:835	milk yield	826:835	Milk fat was negatively related to milk yield and goats on rangeland with 334 mm had the greatest milk fat content (P < 0.001).
32989628	12	70	contain	had	1589:1591	arg2	effects					1601:1607	adverse effects	1593:1607	adverse effects	1593:1607	Nevertheless, slow growth to weaning of offspring had adverse effects on time to reach adequate market weight.
32989628	12	70	contain	had	1589:1591	arg1	growth					1558:1563	slow growth	1553:1563	slow growth to weaning of offspring	1553:1587	Nevertheless, slow growth to weaning of offspring had adverse effects on time to reach adequate market weight.
32989628	1	71	from	rainfall	314:321	arg1	22-25°N					343:349	22-25°N	343:349	22-25°N	343:349	The objective was to characterize milk yield and composition from mixed-breed goats kidding in winter and grazing rangelands with 221 (n = 15), 334 (n = 20), or 441 (n = 12) mm of annual rainfall in northern Mexico (22-25°N).
32989628	1	71	from	rainfall	314:321	arg1	Mexico					335:340	northern Mexico	326:340	northern Mexico (22-25°N)	326:350	The objective was to characterize milk yield and composition from mixed-breed goats kidding in winter and grazing rangelands with 221 (n = 15), 334 (n = 20), or 441 (n = 12) mm of annual rainfall in northern Mexico (22-25°N).
32989628	12	72	theme	market	1635:1640	arg1	weight					1642:1647	adequate market weight	1626:1647	adequate market weight	1626:1647	Nevertheless, slow growth to weaning of offspring had adverse effects on time to reach adequate market weight.
32989628	8	73	theme	other	1126:1130	arg1	rangelands					1132:1141	other rangelands	1126:1141	other rangelands	1126:1141	Goat kids on rangelands with 221 and 441 mm grew faster and were heavier (P < 0.001) at weaning than kids reared in other rangelands.
32989628	10	74	theme	reduced	1389:1395	arg1	precipitation					1404:1416	reduced annual precipitation	1389:1416	reduced annual precipitation in rangelands of northern Mexico	1389:1449	In conclusion, moderate milk yield of mixed-breed goats on rangeland is feasible with parturitions in winter, and milk yield is not affected by reduced annual precipitation in rangelands of northern Mexico.
32989628	1	75	theme	annual	307:312	arg1	rainfall					314:321	annual rainfall	307:321	annual rainfall in northern Mexico (22-25°N)	307:350	The objective was to characterize milk yield and composition from mixed-breed goats kidding in winter and grazing rangelands with 221 (n = 15), 334 (n = 20), or 441 (n = 12) mm of annual rainfall in northern Mexico (22-25°N).
32989628	6	76	theme	fat	894:896	arg1	P < 0.001					907:915	P < 0.001	907:915	P < 0.001	907:915	Milk fat was negatively related to milk yield and goats on rangeland with 334 mm had the greatest milk fat content (P < 0.001).
32989628	6	76	theme	fat	894:896	arg1	content					898:904	milk fat content	889:904	the greatest milk fat content (P < 0.001)	876:916	Milk fat was negatively related to milk yield and goats on rangeland with 334 mm had the greatest milk fat content (P < 0.001).
32989628	3	77	dep	334 mm	582:587	arg1	1.8 kg					590:595	1.8 kg	590:595	1.8 kg; P < 0.01	590:605	Body weight loss during lactation was the highest (6.4 kg) for goats on rangeland with 441 mm of rainfall than goats on 221 mm (1.6 kg), or 334 mm (1.8 kg; P < 0.01).
32989628	6	78	theme	greatest	880:887	arg1	P < 0.001					907:915	P < 0.001	907:915	P < 0.001	907:915	Milk fat was negatively related to milk yield and goats on rangeland with 334 mm had the greatest milk fat content (P < 0.001).
32989628	6	78	theme	greatest	880:887	arg1	content					898:904	milk fat content	889:904	the greatest milk fat content (P < 0.001)	876:916	Milk fat was negatively related to milk yield and goats on rangeland with 334 mm had the greatest milk fat content (P < 0.001).
32989628	2	79	theme	Weekly	353:358	arg1	yield					365:369	Weekly milk yield	353:369	Weekly milk yield	353:369	Weekly milk yield and composition were recorded and progeny growth performance assessed.
32989628	4	80	theme	daily	621:625	arg1	yield					632:636	The highest daily milk yield	609:636	The highest daily milk yield	609:636	The highest daily milk yield was for goats on 221 mm (498 mL; P < 0.01).
32989628	0	81	theme	body	31:34	arg1	weight					36:41	body weight	31:41	body weight	31:41	Milk yield and composition and body weight of offsprings of mixed-breed goats on semi-arid rangelands with different rainfall.
32989628	3	82	dep	1.8 kg	590:595	arg1	P < 0.01					598:605	P < 0.01	598:605	1.8 kg; P < 0.01	590:605	Body weight loss during lactation was the highest (6.4 kg) for goats on rangeland with 441 mm of rainfall than goats on 221 mm (1.6 kg), or 334 mm (1.8 kg; P < 0.01).
32989628	9	83	theme	Weaning	1144:1150	arg1	weight					1152:1157	Weaning weight	1144:1157	Weaning weight	1144:1157	Weaning weight was positively related to milk yield (P < 0.001); but not milk components (P > 0.05).
32989628	4	84	dep	498 mL	663:668	arg1	P < 0.01					671:678	P < 0.01	671:678	498 mL; P < 0.01	663:678	The highest daily milk yield was for goats on 221 mm (498 mL; P < 0.01).
32989628	10	85	theme	moderate	1260:1267	arg1	yield					1274:1278	moderate milk yield	1260:1278	moderate milk yield of mixed-breed goats on rangeland	1260:1312	In conclusion, moderate milk yield of mixed-breed goats on rangeland is feasible with parturitions in winter, and milk yield is not affected by reduced annual precipitation in rangelands of northern Mexico.
32989628	11	86	theme	growth	1462:1467	arg1	performance					1469:1479	Offspring growth performance	1452:1479	Offspring growth performance	1452:1479	Offspring growth performance depends on maternal milk yield, but not milk composition.
32989628	10	87	theme	northern	1435:1442	arg1	Mexico					1444:1449	northern Mexico	1435:1449	northern Mexico	1435:1449	In conclusion, moderate milk yield of mixed-breed goats on rangeland is feasible with parturitions in winter, and milk yield is not affected by reduced annual precipitation in rangelands of northern Mexico.
32989628	1	88	theme	winter	222:227	arg1	rangelands					241:250	winter and grazing rangelands	222:250	winter and grazing rangelands with 221 (n = 15), 334 (n = 20), or 441 (n = 12) mm of annual rainfall in northern Mexico (22-25°N)	222:350	The objective was to characterize milk yield and composition from mixed-breed goats kidding in winter and grazing rangelands with 221 (n = 15), 334 (n = 20), or 441 (n = 12) mm of annual rainfall in northern Mexico (22-25°N).
32989628	10	89	from	precipitation	1404:1416	arg1	rangelands					1421:1430	rangelands	1421:1430	rangelands of northern Mexico	1421:1449	In conclusion, moderate milk yield of mixed-breed goats on rangeland is feasible with parturitions in winter, and milk yield is not affected by reduced annual precipitation in rangelands of northern Mexico.
32989628	1	90	theme	grazing	233:239	arg1	rangelands					241:250	winter and grazing rangelands	222:250	winter and grazing rangelands with 221 (n = 15), 334 (n = 20), or 441 (n = 12) mm of annual rainfall in northern Mexico (22-25°N)	222:350	The objective was to characterize milk yield and composition from mixed-breed goats kidding in winter and grazing rangelands with 221 (n = 15), 334 (n = 20), or 441 (n = 12) mm of annual rainfall in northern Mexico (22-25°N).
32989628	9	91	theme	milk	1185:1188	arg1	P < 0.001					1197:1205	P < 0.001	1197:1205	P < 0.001	1197:1205	Weaning weight was positively related to milk yield (P < 0.001); but not milk components (P > 0.05).
32989628	9	91	theme	milk	1185:1188	arg1	yield					1190:1194	milk yield	1185:1194	milk yield (P < 0.001); but not milk components (P > 0.05)	1185:1242	Weaning weight was positively related to milk yield (P < 0.001); but not milk components (P > 0.05).
33998072	2	0	from	differences	191:201	arg1	trainability					206:217	trainability	206:217	trainability	206:217	Do interindividual differences in trainability exist for morphological and molecular skeletal muscle responses to aerobic exercise training?
33998072	12	1	theme	within-subject	2414:2427	arg1	variability					2429:2439	within-subject variability	2414:2439	within-subject variability	2414:2439	Although positive SDIR values - indicating interindividual differences in trainability - were obtained for aerobic capacity outcomes, maximal enzyme activities, capillary density, all fibre-specific outcomes and BNIP3 protein content, the remaining outcomes produced negative SDIR values indicating a large degree of random measurement error and/or within-subject variability.
33998072	10	2	theme	dehydrogenase	1590:1602	arg1	activity					1604:1611	fibre-specific succinate dehydrogenase activity	1565:1611	fibre-specific succinate dehydrogenase activity	1565:1611	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	13	3	theme	individual	2653:2662	arg1	response					2680:2687	individual skeletal muscle response	2653:2687	individual skeletal muscle response to exercise training	2653:2708	Our findings question the interpretation of heterogeneity in observed responses as evidence of interindividual differences in trainability and highlight the importance of including control groups when analysing individual skeletal muscle response to exercise training.
33998072	6	4	theme	differences	871:881	arg1	evidence					843:850	evidence	843:850	evidence of interindividual differences in trainability	843:897	ABSTRACT Studies have interpreted a wide range of morphological and molecular changes in human skeletal muscle as evidence of interindividual differences in trainability.
33998072	11	5	theme	peak	1919:1922	arg1	rate					1929:1932	peak work rate	1919:1932	peak work rate	1919:1932	We also calculated SDIR values for , peak work rate and the onset of blood lactate accumulation for the REL group and a separate group that exercised at the negative talk test stage.
33998072	13	6	theme	groups	2631:2636	arg1	importance					2599:2608	the importance	2595:2608	the importance of including control groups	2595:2636	Our findings question the interpretation of heterogeneity in observed responses as evidence of interindividual differences in trainability and highlight the importance of including control groups when analysing individual skeletal muscle response to exercise training.
33998072	10	7	theme	intramuscular	1636:1648	arg1	triglycerides					1650:1662	intramuscular triglycerides	1636:1662	intramuscular triglycerides	1636:1662	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	5	8	theme	aerobic	702:708	arg1	training					719:726	aerobic exercise training	702:726	aerobic exercise training	702:726	Our findings suggest that it is inappropriate, and perhaps erroneous, to assume that variability in observed responses reflects interindividual differences in trainability in skeletal muscle responses to aerobic exercise training.
33998072	11	9	theme	negative	2039:2046	arg1	stage					2058:2062	the negative talk test stage	2035:2062	the negative talk test stage	2035:2062	We also calculated SDIR values for , peak work rate and the onset of blood lactate accumulation for the REL group and a separate group that exercised at the negative talk test stage.
33998072	8	10	theme	study	1052:1056	arg1	purpose					1029:1035	The purpose	1025:1035	The purpose of the present study	1025:1056	The purpose of the present study was to use the standard deviation of individual response (SDIR ) statistic to test the hypothesis that interindividual differences in trainability are present for some but not all skeletal muscle outcomes.
33998072	5	11	theme	trainability	657:668	arg1	responses					689:697	trainability in skeletal muscle responses	657:697	trainability in skeletal muscle responses to aerobic exercise training	657:726	Our findings suggest that it is inappropriate, and perhaps erroneous, to assume that variability in observed responses reflects interindividual differences in trainability in skeletal muscle responses to aerobic exercise training.
33998072	9	12	theme	Twenty-nine	1264:1274	arg1	age					1305:1307	age	1305:1307	age: 21 ± 2 years; BMI: 24 ± 3 kg/m2 ; ; 45 ± 7 ml/kg/min	1305:1361	Twenty-nine recreationally active males (age: 21 ± 2 years; BMI: 24 ± 3 kg/m2 ; ; 45 ± 7 ml/kg/min) completed 4 weeks of continuous training (REL; n = 14) or control (n = 15).
33998072	9	12	theme	Twenty-nine	1264:1274	arg1	males					1298:1302	Twenty-nine recreationally active males	1264:1302	Twenty-nine recreationally active males (age: 21 ± 2 years; BMI: 24 ± 3 kg/m2 ; ; 45 ± 7 ml/kg/min)	1264:1362	Twenty-nine recreationally active males (age: 21 ± 2 years; BMI: 24 ± 3 kg/m2 ; ; 45 ± 7 ml/kg/min) completed 4 weeks of continuous training (REL; n = 14) or control (n = 15).
33998072	2	13	theme	exercise	294:301	arg1	training					303:310	aerobic exercise training	286:310	aerobic exercise training	286:310	Do interindividual differences in trainability exist for morphological and molecular skeletal muscle responses to aerobic exercise training?
33998072	8	14	theme	muscle	1247:1252	arg1	outcomes					1254:1261	all skeletal muscle outcomes	1234:1261	some but not all skeletal muscle outcomes	1221:1261	The purpose of the present study was to use the standard deviation of individual response (SDIR ) statistic to test the hypothesis that interindividual differences in trainability are present for some but not all skeletal muscle outcomes.
33998072	12	15	theme	capacity	2180:2187	arg1	outcomes					2189:2196	aerobic capacity outcomes	2172:2196	aerobic capacity outcomes	2172:2196	Although positive SDIR values - indicating interindividual differences in trainability - were obtained for aerobic capacity outcomes, maximal enzyme activities, capillary density, all fibre-specific outcomes and BNIP3 protein content, the remaining outcomes produced negative SDIR values indicating a large degree of random measurement error and/or within-subject variability.
33998072	5	16	theme	skeletal	673:680	arg1	muscle					682:687	skeletal muscle	673:687	skeletal muscle	673:687	Our findings suggest that it is inappropriate, and perhaps erroneous, to assume that variability in observed responses reflects interindividual differences in trainability in skeletal muscle responses to aerobic exercise training.
33998072	10	17	theme	enzyme	1448:1453	arg1	activities					1455:1464	Maximal enzyme activities	1440:1464	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase)	1440:1519	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	17	theme	enzyme	1448:1453	arg1	synthase					1475:1482	citrate synthase	1467:1482	citrate synthase	1467:1482	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	17	theme	enzyme	1448:1453	arg1	dehydrogenase					1506:1518	β-hydroxyacyl-CoA dehydrogenase	1488:1518	β-hydroxyacyl-CoA dehydrogenase	1488:1518	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	11	18	theme	REL	1986:1988	arg1	group					1990:1994	the REL group	1982:1994	the REL group	1982:1994	We also calculated SDIR values for , peak work rate and the onset of blood lactate accumulation for the REL group and a separate group that exercised at the negative talk test stage.
33998072	10	19	theme	vastus	1812:1817	arg1	samples					1829:1835	vastus lateralis samples	1812:1835	vastus lateralis samples collected before and after the intervention	1812:1879	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	13	20	from	question	2455:2462	arg1	responses					2512:2520	observed responses	2503:2520	observed responses as evidence of interindividual differences in trainability	2503:2579	Our findings question the interpretation of heterogeneity in observed responses as evidence of interindividual differences in trainability and highlight the importance of including control groups when analysing individual skeletal muscle response to exercise training.
33998072	2	21	theme	molecular	247:255	arg1	responses					273:281	morphological and molecular skeletal muscle responses	229:281	morphological and molecular skeletal muscle responses to aerobic exercise training	229:310	Do interindividual differences in trainability exist for morphological and molecular skeletal muscle responses to aerobic exercise training?
33998072	12	22	from	differences	2124:2134	arg1	trainability					2139:2150	trainability	2139:2150	trainability	2139:2150	Although positive SDIR values - indicating interindividual differences in trainability - were obtained for aerobic capacity outcomes, maximal enzyme activities, capillary density, all fibre-specific outcomes and BNIP3 protein content, the remaining outcomes produced negative SDIR values indicating a large degree of random measurement error and/or within-subject variability.
33998072	3	23	theme	main	325:328	arg1	What					313:316	What	313:316	What	313:316	What is the main finding and its importance?
33998072	3	23	theme	main	325:328	arg1	finding					330:336	the main finding	321:336	the main finding	321:336	What is the main finding and its importance?
33998072	12	24	theme	measurement	2389:2399	arg1	error					2401:2405	random measurement error	2382:2405	random measurement error	2382:2405	Although positive SDIR values - indicating interindividual differences in trainability - were obtained for aerobic capacity outcomes, maximal enzyme activities, capillary density, all fibre-specific outcomes and BNIP3 protein content, the remaining outcomes produced negative SDIR values indicating a large degree of random measurement error and/or within-subject variability.
33998072	4	25	from	differences	374:384	arg1	trainability					389:400	trainability	389:400	trainability	389:400	Interindividual differences in trainability were present for some, but not all, morphological and molecular outcomes included in our study.
33998072	12	26	theme	indicating	2097:2106	arg1	differences					2124:2134	indicating interindividual differences	2097:2134	positive SDIR values - indicating interindividual differences in trainability -	2074:2152	Although positive SDIR values - indicating interindividual differences in trainability - were obtained for aerobic capacity outcomes, maximal enzyme activities, capillary density, all fibre-specific outcomes and BNIP3 protein content, the remaining outcomes produced negative SDIR values indicating a large degree of random measurement error and/or within-subject variability.
33998072	13	27	theme	interindividual	2537:2551	arg1	differences					2553:2563	interindividual differences	2537:2563	interindividual differences in trainability	2537:2579	Our findings question the interpretation of heterogeneity in observed responses as evidence of interindividual differences in trainability and highlight the importance of including control groups when analysing individual skeletal muscle response to exercise training.
33998072	2	28	theme	morphological	229:241	arg1	responses					273:281	morphological and molecular skeletal muscle responses	229:281	morphological and molecular skeletal muscle responses to aerobic exercise training	229:310	Do interindividual differences in trainability exist for morphological and molecular skeletal muscle responses to aerobic exercise training?
33998072	13	29	theme	heterogeneity	2486:2498	arg1	interpretation					2468:2481	the interpretation	2464:2481	the interpretation of heterogeneity	2464:2498	Our findings question the interpretation of heterogeneity in observed responses as evidence of interindividual differences in trainability and highlight the importance of including control groups when analysing individual skeletal muscle response to exercise training.
33998072	5	30	from	variability	583:593	arg1	responses					607:615	observed responses	598:615	observed responses	598:615	Our findings suggest that it is inappropriate, and perhaps erroneous, to assume that variability in observed responses reflects interindividual differences in trainability in skeletal muscle responses to aerobic exercise training.
33998072	12	31	theme	large	2366:2370	arg1	degree					2372:2377	a large degree	2364:2377	a large degree of random measurement error and/or within-subject variability	2364:2439	Although positive SDIR values - indicating interindividual differences in trainability - were obtained for aerobic capacity outcomes, maximal enzyme activities, capillary density, all fibre-specific outcomes and BNIP3 protein content, the remaining outcomes produced negative SDIR values indicating a large degree of random measurement error and/or within-subject variability.
33998072	12	32	theme	positive	2074:2081	arg1	values					2088:2093	positive SDIR values	2074:2093	positive SDIR values - indicating interindividual differences in trainability -	2074:2152	Although positive SDIR values - indicating interindividual differences in trainability - were obtained for aerobic capacity outcomes, maximal enzyme activities, capillary density, all fibre-specific outcomes and BNIP3 protein content, the remaining outcomes produced negative SDIR values indicating a large degree of random measurement error and/or within-subject variability.
33998072	10	33	dep	composition	1552:1562	arg1	glycogen					1668:1675	glycogen	1668:1675	glycogen	1668:1675	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	33	dep	composition	1552:1562	arg1	triglycerides					1650:1662	intramuscular triglycerides	1636:1662	intramuscular triglycerides	1636:1662	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	2	34	theme	muscle	266:271	arg1	responses					273:281	morphological and molecular skeletal muscle responses	229:281	morphological and molecular skeletal muscle responses to aerobic exercise training	229:310	Do interindividual differences in trainability exist for morphological and molecular skeletal muscle responses to aerobic exercise training?
33998072	5	35	from	differences	642:652	arg1	responses					689:697	trainability in skeletal muscle responses	657:697	trainability in skeletal muscle responses to aerobic exercise training	657:726	Our findings suggest that it is inappropriate, and perhaps erroneous, to assume that variability in observed responses reflects interindividual differences in trainability in skeletal muscle responses to aerobic exercise training.
33998072	0	36	from	differences	26:36	arg1	muscle					48:53	select muscle	41:53	select muscle	41:53	Examining interindividual differences in select muscle and whole-body adaptations to continuous endurance training.
33998072	0	36	from	differences	26:36	arg1	adaptations					70:80	whole-body adaptations	59:80	whole-body adaptations to continuous endurance training	59:113	Examining interindividual differences in select muscle and whole-body adaptations to continuous endurance training.
33998072	10	37	theme	β-hydroxyacyl-CoA	1488:1504	arg1	activities					1455:1464	Maximal enzyme activities	1440:1464	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase)	1440:1519	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	37	theme	β-hydroxyacyl-CoA	1488:1504	arg1	dehydrogenase					1506:1518	β-hydroxyacyl-CoA dehydrogenase	1488:1518	β-hydroxyacyl-CoA dehydrogenase	1488:1518	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	13	38	from	evidence	2525:2532	arg1	trainability					2568:2579	trainability	2568:2579	trainability	2568:2579	Our findings question the interpretation of heterogeneity in observed responses as evidence of interindividual differences in trainability and highlight the importance of including control groups when analysing individual skeletal muscle response to exercise training.
33998072	0	39	theme	continuous	85:94	arg1	training					106:113	continuous endurance training	85:113	continuous endurance training	85:113	Examining interindividual differences in select muscle and whole-body adaptations to continuous endurance training.
33998072	1	40	theme	study	165:169	arg1	question					149:156	the central question	137:156	the central question of the study	137:169	NEW FINDINGS What is the central question of the study?
33998072	1	40	theme	study	165:169	arg1	FINDINGS					120:127	NEW FINDINGS	116:127	NEW FINDINGS What is the central question of the study	116:169	NEW FINDINGS What is the central question of the study?
33998072	8	41	from	differences	1177:1187	arg1	trainability					1192:1203	trainability	1192:1203	trainability	1192:1203	The purpose of the present study was to use the standard deviation of individual response (SDIR ) statistic to test the hypothesis that interindividual differences in trainability are present for some but not all skeletal muscle outcomes.
33998072	12	42	theme	SDIR	2341:2344	arg1	values					2346:2351	negative SDIR values	2332:2351	negative SDIR values indicating a large degree of random measurement error and/or within-subject variability	2332:2439	Although positive SDIR values - indicating interindividual differences in trainability - were obtained for aerobic capacity outcomes, maximal enzyme activities, capillary density, all fibre-specific outcomes and BNIP3 protein content, the remaining outcomes produced negative SDIR values indicating a large degree of random measurement error and/or within-subject variability.
33998072	12	43	theme	BNIP3	2277:2281	arg1	content					2291:2297	BNIP3 protein content	2277:2297	BNIP3 protein content	2277:2297	Although positive SDIR values - indicating interindividual differences in trainability - were obtained for aerobic capacity outcomes, maximal enzyme activities, capillary density, all fibre-specific outcomes and BNIP3 protein content, the remaining outcomes produced negative SDIR values indicating a large degree of random measurement error and/or within-subject variability.
33998072	8	44	theme	individual	1095:1104	arg1	statistic					1123:1131	individual response (SDIR ) statistic	1095:1131	individual response (SDIR ) statistic	1095:1131	The purpose of the present study was to use the standard deviation of individual response (SDIR ) statistic to test the hypothesis that interindividual differences in trainability are present for some but not all skeletal muscle outcomes.
33998072	5	45	theme	interindividual	626:640	arg1	differences					642:652	interindividual differences	626:652	interindividual differences in trainability in skeletal muscle responses to aerobic exercise training	626:726	Our findings suggest that it is inappropriate, and perhaps erroneous, to assume that variability in observed responses reflects interindividual differences in trainability in skeletal muscle responses to aerobic exercise training.
33998072	1	46	theme	central	141:147	arg1	question					149:156	the central question	137:156	the central question of the study	137:169	NEW FINDINGS What is the central question of the study?
33998072	1	46	theme	central	141:147	arg1	FINDINGS					120:127	NEW FINDINGS	116:127	NEW FINDINGS What is the central question of the study	116:169	NEW FINDINGS What is the central question of the study?
33998072	10	47	theme	capillary	1522:1530	arg1	density					1532:1538	capillary density	1522:1538	capillary density	1522:1538	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	12	48	theme	variability	2429:2439	arg1	degree					2372:2377	a large degree	2364:2377	a large degree of random measurement error and/or within-subject variability	2364:2439	Although positive SDIR values - indicating interindividual differences in trainability - were obtained for aerobic capacity outcomes, maximal enzyme activities, capillary density, all fibre-specific outcomes and BNIP3 protein content, the remaining outcomes produced negative SDIR values indicating a large degree of random measurement error and/or within-subject variability.
33998072	7	49	theme	within-subject	997:1010	arg1	variability					1012:1022	within-subject variability	997:1022	within-subject variability	997:1022	However, these interpretations fail to account for the influence of random measurement error and within-subject variability.
33998072	6	50	from	evidence	843:850	arg1	trainability					886:897	trainability	886:897	trainability	886:897	ABSTRACT Studies have interpreted a wide range of morphological and molecular changes in human skeletal muscle as evidence of interindividual differences in trainability.
33998072	12	51	theme	remaining	2304:2312	arg1	outcomes					2314:2321	the remaining outcomes	2300:2321	the remaining outcomes	2300:2321	Although positive SDIR values - indicating interindividual differences in trainability - were obtained for aerobic capacity outcomes, maximal enzyme activities, capillary density, all fibre-specific outcomes and BNIP3 protein content, the remaining outcomes produced negative SDIR values indicating a large degree of random measurement error and/or within-subject variability.
33998072	10	52	theme	BCL2-interacting	1705:1720	arg1	protein					1722:1728	BCL2-interacting protein 3	1705:1730	BCL2-interacting protein 3 (BNIP3)	1705:1738	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	52	theme	BCL2-interacting	1705:1720	arg1	BNIP3					1733:1737	BNIP3	1733:1737	BNIP3	1733:1737	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	52	theme	BCL2-interacting	1705:1720	arg1	markers					1683:1689	markers	1683:1689	markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1)	1683:1793	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	9	53	theme	training	1396:1403	arg1	4 weeks					1374:1380	4 weeks	1374:1380	4 weeks of continuous training (REL; n = 14) or control (n = 15)	1374:1437	Twenty-nine recreationally active males (age: 21 ± 2 years; BMI: 24 ± 3 kg/m2 ; ; 45 ± 7 ml/kg/min) completed 4 weeks of continuous training (REL; n = 14) or control (n = 15).
33998072	7	54	theme	error	987:991	arg1	influence					955:963	the influence	951:963	the influence of random measurement error and within-subject variability	951:1022	However, these interpretations fail to account for the influence of random measurement error and within-subject variability.
33998072	10	55	theme	type	1547:1550	arg1	composition					1552:1562	fibre type composition	1541:1562	fibre type composition	1541:1562	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	11	56	theme	accumulation	1965:1976	arg1	values					1906:1911	SDIR values	1901:1911	SDIR values for	1901:1915	We also calculated SDIR values for , peak work rate and the onset of blood lactate accumulation for the REL group and a separate group that exercised at the negative talk test stage.
33998072	11	56	theme	accumulation	1965:1976	arg1	onset					1942:1946	the onset	1938:1946	the onset of blood lactate accumulation	1938:1976	We also calculated SDIR values for , peak work rate and the onset of blood lactate accumulation for the REL group and a separate group that exercised at the negative talk test stage.
33998072	11	56	theme	accumulation	1965:1976	arg1	rate					1929:1932	peak work rate	1919:1932	peak work rate	1919:1932	We also calculated SDIR values for , peak work rate and the onset of blood lactate accumulation for the REL group and a separate group that exercised at the negative talk test stage.
33998072	2	57	theme	interindividual	175:189	arg1	differences					191:201	interindividual differences	175:201	interindividual differences in trainability	175:217	Do interindividual differences in trainability exist for morphological and molecular skeletal muscle responses to aerobic exercise training?
33998072	11	58	theme	blood	1951:1955	arg1	accumulation					1965:1976	blood lactate accumulation	1951:1976	blood lactate accumulation	1951:1976	We also calculated SDIR values for , peak work rate and the onset of blood lactate accumulation for the REL group and a separate group that exercised at the negative talk test stage.
33998072	0	59	theme	select	41:46	arg1	muscle					48:53	select muscle	41:53	select muscle	41:53	Examining interindividual differences in select muscle and whole-body adaptations to continuous endurance training.
33998072	13	60	theme	muscle	2673:2678	arg1	response					2680:2687	individual skeletal muscle response	2653:2687	individual skeletal muscle response to exercise training	2653:2708	Our findings question the interpretation of heterogeneity in observed responses as evidence of interindividual differences in trainability and highlight the importance of including control groups when analysing individual skeletal muscle response to exercise training.
33998072	6	61	from	muscle	833:838	arg1	range					770:774	a wide range	763:774	a wide range of morphological and molecular changes in human skeletal muscle	763:838	ABSTRACT Studies have interpreted a wide range of morphological and molecular changes in human skeletal muscle as evidence of interindividual differences in trainability.
33998072	10	62	theme	succinate	1580:1588	arg1	dehydrogenase					1590:1602	fibre-specific succinate dehydrogenase	1565:1602	fibre-specific succinate dehydrogenase activity	1565:1611	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	6	63	theme	changes	807:813	arg1	range					770:774	a wide range	763:774	a wide range of morphological and molecular changes in human skeletal muscle	763:838	ABSTRACT Studies have interpreted a wide range of morphological and molecular changes in human skeletal muscle as evidence of interindividual differences in trainability.
33998072	12	64	theme	negative	2332:2339	arg1	values					2346:2351	negative SDIR values	2332:2351	negative SDIR values indicating a large degree of random measurement error and/or within-subject variability	2332:2439	Although positive SDIR values - indicating interindividual differences in trainability - were obtained for aerobic capacity outcomes, maximal enzyme activities, capillary density, all fibre-specific outcomes and BNIP3 protein content, the remaining outcomes produced negative SDIR values indicating a large degree of random measurement error and/or within-subject variability.
33998072	11	65	theme	separate	2002:2009	arg1	group					2011:2015	a separate group	2000:2015	a separate group that exercised at the negative talk test stage	2000:2062	We also calculated SDIR values for , peak work rate and the onset of blood lactate accumulation for the REL group and a separate group that exercised at the negative talk test stage.
33998072	7	66	theme	measurement	975:985	arg1	error					987:991	random measurement error	968:991	random measurement error	968:991	However, these interpretations fail to account for the influence of random measurement error and within-subject variability.
33998072	6	67	from	changes	807:813	arg1	muscle					833:838	human skeletal muscle	818:838	human skeletal muscle	818:838	ABSTRACT Studies have interpreted a wide range of morphological and molecular changes in human skeletal muscle as evidence of interindividual differences in trainability.
33998072	10	68	theme	substrate	1617:1625	arg1	storage					1627:1633	substrate storage	1617:1633	substrate storage	1617:1633	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	12	69	theme	enzyme	2207:2212	arg1	activities					2214:2223	maximal enzyme activities	2199:2223	maximal enzyme activities	2199:2223	Although positive SDIR values - indicating interindividual differences in trainability - were obtained for aerobic capacity outcomes, maximal enzyme activities, capillary density, all fibre-specific outcomes and BNIP3 protein content, the remaining outcomes produced negative SDIR values indicating a large degree of random measurement error and/or within-subject variability.
33998072	11	70	theme	work	1924:1927	arg1	rate					1929:1932	peak work rate	1919:1932	peak work rate	1919:1932	We also calculated SDIR values for , peak work rate and the onset of blood lactate accumulation for the REL group and a separate group that exercised at the negative talk test stage.
33998072	10	71	dep	activities	1455:1464	arg1	activities					1455:1464	Maximal enzyme activities	1440:1464	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase)	1440:1519	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	71	dep	activities	1455:1464	arg1	synthase					1475:1482	citrate synthase	1467:1482	citrate synthase	1467:1482	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	71	dep	activities	1455:1464	arg1	dehydrogenase					1506:1518	β-hydroxyacyl-CoA dehydrogenase	1488:1518	β-hydroxyacyl-CoA dehydrogenase	1488:1518	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	9	72	dep	age	1305:1307	arg1	45 ± 7 ml/kg/min					1346:1361	45 ± 7 ml/kg/min	1346:1361	age: 21 ± 2 years; BMI: 24 ± 3 kg/m2 ; ; 45 ± 7 ml/kg/min	1305:1361	Twenty-nine recreationally active males (age: 21 ± 2 years; BMI: 24 ± 3 kg/m2 ; ; 45 ± 7 ml/kg/min) completed 4 weeks of continuous training (REL; n = 14) or control (n = 15).
33998072	9	72	dep	age	1305:1307	arg1	BMI					1324:1326	BMI	1324:1326	BMI	1324:1326	Twenty-nine recreationally active males (age: 21 ± 2 years; BMI: 24 ± 3 kg/m2 ; ; 45 ± 7 ml/kg/min) completed 4 weeks of continuous training (REL; n = 14) or control (n = 15).
33998072	9	72	dep	age	1305:1307	arg1	21 ± 2 years					1310:1321	21 ± 2 years	1310:1321	age: 21 ± 2 years; BMI: 24 ± 3 kg/m2 ; ; 45 ± 7 ml/kg/min	1305:1361	Twenty-nine recreationally active males (age: 21 ± 2 years; BMI: 24 ± 3 kg/m2 ; ; 45 ± 7 ml/kg/min) completed 4 weeks of continuous training (REL; n = 14) or control (n = 15).
33998072	10	73	dep	markers	1683:1689	arg1	kinase					1785:1790	PTEN-induced kinase 1	1772:1792	PTEN-induced kinase 1	1772:1792	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	73	dep	markers	1683:1689	arg1	protein					1722:1728	BCL2-interacting protein 3	1705:1730	BCL2-interacting protein 3 (BNIP3)	1705:1738	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	73	dep	markers	1683:1689	arg1	protein					1752:1758	BNIP3-like protein	1741:1758	BNIP3-like protein	1741:1758	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	73	dep	markers	1683:1689	arg1	markers					1683:1689	markers	1683:1689	markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1)	1683:1793	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	73	dep	markers	1683:1689	arg1	parkin					1761:1766	parkin	1761:1766	parkin	1761:1766	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	73	dep	markers	1683:1689	arg1	BNIP3					1733:1737	BNIP3	1733:1737	BNIP3	1733:1737	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	6	74	theme	wide	765:768	arg1	range					770:774	a wide range	763:774	a wide range of morphological and molecular changes in human skeletal muscle	763:838	ABSTRACT Studies have interpreted a wide range of morphological and molecular changes in human skeletal muscle as evidence of interindividual differences in trainability.
33998072	10	75	theme	PTEN-induced	1772:1783	arg1	markers					1683:1689	markers	1683:1689	markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1)	1683:1793	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	75	theme	PTEN-induced	1772:1783	arg1	kinase					1785:1790	PTEN-induced kinase 1	1772:1792	PTEN-induced kinase 1	1772:1792	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	8	76	theme	present	1044:1050	arg1	study					1052:1056	the present study	1040:1056	the present study	1040:1056	The purpose of the present study was to use the standard deviation of individual response (SDIR ) statistic to test the hypothesis that interindividual differences in trainability are present for some but not all skeletal muscle outcomes.
33998072	6	77	theme	interindividual	855:869	arg1	differences					871:881	interindividual differences	855:881	interindividual differences in trainability	855:897	ABSTRACT Studies have interpreted a wide range of morphological and molecular changes in human skeletal muscle as evidence of interindividual differences in trainability.
33998072	9	78	dep	BMI	1324:1326	arg1	24 ± 3 kg/m2					1329:1340	24 ± 3 kg/m2	1329:1340	age: 21 ± 2 years; BMI: 24 ± 3 kg/m2 ; ; 45 ± 7 ml/kg/min	1305:1361	Twenty-nine recreationally active males (age: 21 ± 2 years; BMI: 24 ± 3 kg/m2 ; ; 45 ± 7 ml/kg/min) completed 4 weeks of continuous training (REL; n = 14) or control (n = 15).
33998072	13	79	theme	including	2613:2621	arg1	groups					2631:2636	including control groups	2613:2636	including control groups	2613:2636	Our findings question the interpretation of heterogeneity in observed responses as evidence of interindividual differences in trainability and highlight the importance of including control groups when analysing individual skeletal muscle response to exercise training.
33998072	8	80	theme	skeletal	1238:1245	arg1	outcomes					1254:1261	all skeletal muscle outcomes	1234:1261	some but not all skeletal muscle outcomes	1221:1261	The purpose of the present study was to use the standard deviation of individual response (SDIR ) statistic to test the hypothesis that interindividual differences in trainability are present for some but not all skeletal muscle outcomes.
33998072	13	81	theme	control	2623:2629	arg1	groups					2631:2636	including control groups	2613:2636	including control groups	2613:2636	Our findings question the interpretation of heterogeneity in observed responses as evidence of interindividual differences in trainability and highlight the importance of including control groups when analysing individual skeletal muscle response to exercise training.
33998072	8	82	theme	standard	1073:1080	arg1	deviation					1082:1090	the standard deviation	1069:1090	the standard deviation of individual response (SDIR ) statistic	1069:1131	The purpose of the present study was to use the standard deviation of individual response (SDIR ) statistic to test the hypothesis that interindividual differences in trainability are present for some but not all skeletal muscle outcomes.
33998072	12	83	theme	aerobic	2172:2178	arg1	outcomes					2189:2196	aerobic capacity outcomes	2172:2196	aerobic capacity outcomes	2172:2196	Although positive SDIR values - indicating interindividual differences in trainability - were obtained for aerobic capacity outcomes, maximal enzyme activities, capillary density, all fibre-specific outcomes and BNIP3 protein content, the remaining outcomes produced negative SDIR values indicating a large degree of random measurement error and/or within-subject variability.
33998072	6	84	theme	skeletal	824:831	arg1	muscle					833:838	human skeletal muscle	818:838	human skeletal muscle	818:838	ABSTRACT Studies have interpreted a wide range of morphological and molecular changes in human skeletal muscle as evidence of interindividual differences in trainability.
33998072	6	85	theme	human	818:822	arg1	muscle					833:838	human skeletal muscle	818:838	human skeletal muscle	818:838	ABSTRACT Studies have interpreted a wide range of morphological and molecular changes in human skeletal muscle as evidence of interindividual differences in trainability.
33998072	11	86	theme	talk	2048:2051	arg1	stage					2058:2062	the negative talk test stage	2035:2062	the negative talk test stage	2035:2062	We also calculated SDIR values for , peak work rate and the onset of blood lactate accumulation for the REL group and a separate group that exercised at the negative talk test stage.
33998072	12	87	theme	random	2382:2387	arg1	error					2401:2405	random measurement error	2382:2405	random measurement error	2382:2405	Although positive SDIR values - indicating interindividual differences in trainability - were obtained for aerobic capacity outcomes, maximal enzyme activities, capillary density, all fibre-specific outcomes and BNIP3 protein content, the remaining outcomes produced negative SDIR values indicating a large degree of random measurement error and/or within-subject variability.
33998072	5	88	dep	inappropriate	530:542	arg1	erroneous					557:565	erroneous	557:565	erroneous	557:565	Our findings suggest that it is inappropriate, and perhaps erroneous, to assume that variability in observed responses reflects interindividual differences in trainability in skeletal muscle responses to aerobic exercise training.
33998072	6	89	from	trainability	886:897	arg1	evidence					843:850	evidence	843:850	evidence of interindividual differences in trainability	843:897	ABSTRACT Studies have interpreted a wide range of morphological and molecular changes in human skeletal muscle as evidence of interindividual differences in trainability.
33998072	2	90	theme	skeletal	257:264	arg1	responses					273:281	morphological and molecular skeletal muscle responses	229:281	morphological and molecular skeletal muscle responses to aerobic exercise training	229:310	Do interindividual differences in trainability exist for morphological and molecular skeletal muscle responses to aerobic exercise training?
33998072	12	91	theme	error	2401:2405	arg1	degree					2372:2377	a large degree	2364:2377	a large degree of random measurement error and/or within-subject variability	2364:2439	Although positive SDIR values - indicating interindividual differences in trainability - were obtained for aerobic capacity outcomes, maximal enzyme activities, capillary density, all fibre-specific outcomes and BNIP3 protein content, the remaining outcomes produced negative SDIR values indicating a large degree of random measurement error and/or within-subject variability.
33998072	5	92	theme	exercise	710:717	arg1	training					719:726	aerobic exercise training	702:726	aerobic exercise training	702:726	Our findings suggest that it is inappropriate, and perhaps erroneous, to assume that variability in observed responses reflects interindividual differences in trainability in skeletal muscle responses to aerobic exercise training.
33998072	4	93	theme	morphological	438:450	arg1	some					419:422	some	419:422	some	419:422	Interindividual differences in trainability were present for some, but not all, morphological and molecular outcomes included in our study.
33998072	4	93	theme	morphological	438:450	arg1	outcomes					466:473	morphological and molecular outcomes	438:473	morphological and molecular outcomes included in our study	438:495	Interindividual differences in trainability were present for some, but not all, morphological and molecular outcomes included in our study.
33998072	10	94	dep	vastus	1812:1817	arg1	lateralis					1819:1827	lateralis	1819:1827	lateralis	1819:1827	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	95	theme	Maximal	1440:1446	arg1	activities					1455:1464	Maximal enzyme activities	1440:1464	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase)	1440:1519	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	95	theme	Maximal	1440:1446	arg1	synthase					1475:1482	citrate synthase	1467:1482	citrate synthase	1467:1482	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	95	theme	Maximal	1440:1446	arg1	dehydrogenase					1506:1518	β-hydroxyacyl-CoA dehydrogenase	1488:1518	β-hydroxyacyl-CoA dehydrogenase	1488:1518	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	13	96	theme	differences	2553:2563	arg1	evidence					2525:2532	evidence	2525:2532	evidence of interindividual differences in trainability	2525:2579	Our findings question the interpretation of heterogeneity in observed responses as evidence of interindividual differences in trainability and highlight the importance of including control groups when analysing individual skeletal muscle response to exercise training.
33998072	2	97	theme	aerobic	286:292	arg1	training					303:310	aerobic exercise training	286:310	aerobic exercise training	286:310	Do interindividual differences in trainability exist for morphological and molecular skeletal muscle responses to aerobic exercise training?
33998072	13	98	from	differences	2553:2563	arg1	trainability					2568:2579	trainability	2568:2579	trainability	2568:2579	Our findings question the interpretation of heterogeneity in observed responses as evidence of interindividual differences in trainability and highlight the importance of including control groups when analysing individual skeletal muscle response to exercise training.
33998072	4	99	theme	molecular	456:464	arg1	some					419:422	some	419:422	some	419:422	Interindividual differences in trainability were present for some, but not all, morphological and molecular outcomes included in our study.
33998072	4	99	theme	molecular	456:464	arg1	outcomes					466:473	morphological and molecular outcomes	438:473	morphological and molecular outcomes included in our study	438:495	Interindividual differences in trainability were present for some, but not all, morphological and molecular outcomes included in our study.
33998072	13	100	theme	observed	2503:2510	arg1	responses					2512:2520	observed responses	2503:2520	observed responses as evidence of interindividual differences in trainability	2503:2579	Our findings question the interpretation of heterogeneity in observed responses as evidence of interindividual differences in trainability and highlight the importance of including control groups when analysing individual skeletal muscle response to exercise training.
33998072	12	101	theme	interindividual	2108:2122	arg1	differences					2124:2134	indicating interindividual differences	2097:2134	positive SDIR values - indicating interindividual differences in trainability -	2074:2152	Although positive SDIR values - indicating interindividual differences in trainability - were obtained for aerobic capacity outcomes, maximal enzyme activities, capillary density, all fibre-specific outcomes and BNIP3 protein content, the remaining outcomes produced negative SDIR values indicating a large degree of random measurement error and/or within-subject variability.
33998072	9	102	dep	REL	1406:1408	arg1	n = 14					1411:1416	n = 14	1411:1416	n = 14	1411:1416	Twenty-nine recreationally active males (age: 21 ± 2 years; BMI: 24 ± 3 kg/m2 ; ; 45 ± 7 ml/kg/min) completed 4 weeks of continuous training (REL; n = 14) or control (n = 15).
33998072	0	103	theme	endurance	96:104	arg1	training					106:113	continuous endurance training	85:113	continuous endurance training	85:113	Examining interindividual differences in select muscle and whole-body adaptations to continuous endurance training.
33998072	9	104	dep	training	1396:1403	arg1	REL					1406:1408	REL	1406:1408	REL; n = 14	1406:1416	Twenty-nine recreationally active males (age: 21 ± 2 years; BMI: 24 ± 3 kg/m2 ; ; 45 ± 7 ml/kg/min) completed 4 weeks of continuous training (REL; n = 14) or control (n = 15).
33998072	12	105	theme	SDIR	2083:2086	arg1	values					2088:2093	positive SDIR values	2074:2093	positive SDIR values - indicating interindividual differences in trainability -	2074:2152	Although positive SDIR values - indicating interindividual differences in trainability - were obtained for aerobic capacity outcomes, maximal enzyme activities, capillary density, all fibre-specific outcomes and BNIP3 protein content, the remaining outcomes produced negative SDIR values indicating a large degree of random measurement error and/or within-subject variability.
33998072	8	106	theme	response	1106:1113	arg1	statistic					1123:1131	individual response (SDIR ) statistic	1095:1131	individual response (SDIR ) statistic	1095:1131	The purpose of the present study was to use the standard deviation of individual response (SDIR ) statistic to test the hypothesis that interindividual differences in trainability are present for some but not all skeletal muscle outcomes.
33998072	12	107	dep	values	2088:2093	arg1	differences					2124:2134	indicating interindividual differences	2097:2134	positive SDIR values - indicating interindividual differences in trainability -	2074:2152	Although positive SDIR values - indicating interindividual differences in trainability - were obtained for aerobic capacity outcomes, maximal enzyme activities, capillary density, all fibre-specific outcomes and BNIP3 protein content, the remaining outcomes produced negative SDIR values indicating a large degree of random measurement error and/or within-subject variability.
33998072	11	108	theme	test	2053:2056	arg1	stage					2058:2062	the negative talk test stage	2035:2062	the negative talk test stage	2035:2062	We also calculated SDIR values for , peak work rate and the onset of blood lactate accumulation for the REL group and a separate group that exercised at the negative talk test stage.
33998072	13	109	from	trainability	2568:2579	arg1	evidence					2525:2532	evidence	2525:2532	evidence of interindividual differences in trainability	2525:2579	Our findings question the interpretation of heterogeneity in observed responses as evidence of interindividual differences in trainability and highlight the importance of including control groups when analysing individual skeletal muscle response to exercise training.
33998072	10	110	theme	citrate	1467:1473	arg1	activities					1455:1464	Maximal enzyme activities	1440:1464	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase)	1440:1519	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	110	theme	citrate	1467:1473	arg1	synthase					1475:1482	citrate synthase	1467:1482	citrate synthase	1467:1482	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	11	111	theme	SDIR	1901:1904	arg1	values					1906:1911	SDIR values	1901:1911	SDIR values for	1901:1915	We also calculated SDIR values for , peak work rate and the onset of blood lactate accumulation for the REL group and a separate group that exercised at the negative talk test stage.
33998072	8	112	theme	SDIR	1116:1119	arg1	statistic					1123:1131	individual response (SDIR ) statistic	1095:1131	individual response (SDIR ) statistic	1095:1131	The purpose of the present study was to use the standard deviation of individual response (SDIR ) statistic to test the hypothesis that interindividual differences in trainability are present for some but not all skeletal muscle outcomes.
33998072	10	113	theme	mitophagy	1694:1702	arg1	kinase					1785:1790	PTEN-induced kinase 1	1772:1792	PTEN-induced kinase 1	1772:1792	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	113	theme	mitophagy	1694:1702	arg1	synthase					1475:1482	citrate synthase	1467:1482	citrate synthase	1467:1482	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	113	theme	mitophagy	1694:1702	arg1	storage					1627:1633	substrate storage	1617:1633	substrate storage	1617:1633	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	113	theme	mitophagy	1694:1702	arg1	protein					1722:1728	BCL2-interacting protein 3	1705:1730	BCL2-interacting protein 3 (BNIP3)	1705:1738	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	113	theme	mitophagy	1694:1702	arg1	density					1532:1538	capillary density	1522:1538	capillary density	1522:1538	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	113	theme	mitophagy	1694:1702	arg1	protein					1752:1758	BNIP3-like protein	1741:1758	BNIP3-like protein	1741:1758	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	113	theme	mitophagy	1694:1702	arg1	markers					1683:1689	markers	1683:1689	markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1)	1683:1793	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	113	theme	mitophagy	1694:1702	arg1	parkin					1761:1766	parkin	1761:1766	parkin	1761:1766	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	113	theme	mitophagy	1694:1702	arg1	activities					1455:1464	Maximal enzyme activities	1440:1464	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase)	1440:1519	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	113	theme	mitophagy	1694:1702	arg1	activity					1604:1611	fibre-specific succinate dehydrogenase activity	1565:1611	fibre-specific succinate dehydrogenase activity	1565:1611	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	113	theme	mitophagy	1694:1702	arg1	dehydrogenase					1506:1518	β-hydroxyacyl-CoA dehydrogenase	1488:1518	β-hydroxyacyl-CoA dehydrogenase	1488:1518	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	113	theme	mitophagy	1694:1702	arg1	composition					1552:1562	fibre type composition	1541:1562	fibre type composition	1541:1562	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	12	114	theme	protein	2283:2289	arg1	content					2291:2297	BNIP3 protein content	2277:2297	BNIP3 protein content	2277:2297	Although positive SDIR values - indicating interindividual differences in trainability - were obtained for aerobic capacity outcomes, maximal enzyme activities, capillary density, all fibre-specific outcomes and BNIP3 protein content, the remaining outcomes produced negative SDIR values indicating a large degree of random measurement error and/or within-subject variability.
33998072	0	115	theme	interindividual	10:24	arg1	differences					26:36	interindividual differences	10:36	interindividual differences in select muscle and whole-body adaptations to continuous endurance training	10:113	Examining interindividual differences in select muscle and whole-body adaptations to continuous endurance training.
33998072	9	116	theme	continuous	1385:1394	arg1	training					1396:1403	continuous training	1385:1403	continuous training (REL; n = 14)	1385:1417	Twenty-nine recreationally active males (age: 21 ± 2 years; BMI: 24 ± 3 kg/m2 ; ; 45 ± 7 ml/kg/min) completed 4 weeks of continuous training (REL; n = 14) or control (n = 15).
33998072	8	117	theme	statistic	1123:1131	arg1	deviation					1082:1090	the standard deviation	1069:1090	the standard deviation of individual response (SDIR ) statistic	1069:1131	The purpose of the present study was to use the standard deviation of individual response (SDIR ) statistic to test the hypothesis that interindividual differences in trainability are present for some but not all skeletal muscle outcomes.
33998072	7	118	theme	variability	1012:1022	arg1	influence					955:963	the influence	951:963	the influence of random measurement error and within-subject variability	951:1022	However, these interpretations fail to account for the influence of random measurement error and within-subject variability.
33998072	6	119	from	differences	871:881	arg1	trainability					886:897	trainability	886:897	trainability	886:897	ABSTRACT Studies have interpreted a wide range of morphological and molecular changes in human skeletal muscle as evidence of interindividual differences in trainability.
33998072	6	120	theme	ABSTRACT	729:736	arg1	Studies					738:744	ABSTRACT Studies	729:744	ABSTRACT Studies	729:744	ABSTRACT Studies have interpreted a wide range of morphological and molecular changes in human skeletal muscle as evidence of interindividual differences in trainability.
33998072	5	121	theme	observed	598:605	arg1	responses					607:615	observed responses	598:615	observed responses	598:615	Our findings suggest that it is inappropriate, and perhaps erroneous, to assume that variability in observed responses reflects interindividual differences in trainability in skeletal muscle responses to aerobic exercise training.
33998072	1	122	theme	NEW	116:118	arg1	question					149:156	the central question	137:156	the central question of the study	137:169	NEW FINDINGS What is the central question of the study?
33998072	1	122	theme	NEW	116:118	arg1	FINDINGS					120:127	NEW FINDINGS	116:127	NEW FINDINGS What is the central question of the study	116:169	NEW FINDINGS What is the central question of the study?
33998072	9	123	theme	active	1291:1296	arg1	age					1305:1307	age	1305:1307	age: 21 ± 2 years; BMI: 24 ± 3 kg/m2 ; ; 45 ± 7 ml/kg/min	1305:1361	Twenty-nine recreationally active males (age: 21 ± 2 years; BMI: 24 ± 3 kg/m2 ; ; 45 ± 7 ml/kg/min) completed 4 weeks of continuous training (REL; n = 14) or control (n = 15).
33998072	9	123	theme	active	1291:1296	arg1	males					1298:1302	Twenty-nine recreationally active males	1264:1302	Twenty-nine recreationally active males (age: 21 ± 2 years; BMI: 24 ± 3 kg/m2 ; ; 45 ± 7 ml/kg/min)	1264:1362	Twenty-nine recreationally active males (age: 21 ± 2 years; BMI: 24 ± 3 kg/m2 ; ; 45 ± 7 ml/kg/min) completed 4 weeks of continuous training (REL; n = 14) or control (n = 15).
33998072	10	124	theme	fibre	1541:1545	arg1	composition					1552:1562	fibre type composition	1541:1562	fibre type composition	1541:1562	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	12	125	theme	fibre-specific	2249:2262	arg1	outcomes					2264:2271	all fibre-specific outcomes	2245:2271	all fibre-specific outcomes	2245:2271	Although positive SDIR values - indicating interindividual differences in trainability - were obtained for aerobic capacity outcomes, maximal enzyme activities, capillary density, all fibre-specific outcomes and BNIP3 protein content, the remaining outcomes produced negative SDIR values indicating a large degree of random measurement error and/or within-subject variability.
33998072	13	126	theme	skeletal	2664:2671	arg1	response					2680:2687	individual skeletal muscle response	2653:2687	individual skeletal muscle response to exercise training	2653:2708	Our findings question the interpretation of heterogeneity in observed responses as evidence of interindividual differences in trainability and highlight the importance of including control groups when analysing individual skeletal muscle response to exercise training.
33998072	5	127	from	trainability	657:668	arg1	muscle					682:687	skeletal muscle	673:687	skeletal muscle	673:687	Our findings suggest that it is inappropriate, and perhaps erroneous, to assume that variability in observed responses reflects interindividual differences in trainability in skeletal muscle responses to aerobic exercise training.
33998072	11	128	theme	lactate	1957:1963	arg1	accumulation					1965:1976	blood lactate accumulation	1951:1976	blood lactate accumulation	1951:1976	We also calculated SDIR values for , peak work rate and the onset of blood lactate accumulation for the REL group and a separate group that exercised at the negative talk test stage.
33998072	0	129	theme	whole-body	59:68	arg1	adaptations					70:80	whole-body adaptations	59:80	whole-body adaptations to continuous endurance training	59:113	Examining interindividual differences in select muscle and whole-body adaptations to continuous endurance training.
33998072	8	130	theme	interindividual	1161:1175	arg1	differences					1177:1187	interindividual differences	1161:1187	interindividual differences in trainability	1161:1203	The purpose of the present study was to use the standard deviation of individual response (SDIR ) statistic to test the hypothesis that interindividual differences in trainability are present for some but not all skeletal muscle outcomes.
33998072	4	131	theme	Interindividual	358:372	arg1	differences					374:384	Interindividual differences	358:384	Interindividual differences in trainability	358:400	Interindividual differences in trainability were present for some, but not all, morphological and molecular outcomes included in our study.
33998072	10	132	theme	fibre-specific	1565:1578	arg1	dehydrogenase					1590:1602	fibre-specific succinate dehydrogenase	1565:1602	fibre-specific succinate dehydrogenase activity	1565:1611	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	12	133	theme	capillary	2226:2234	arg1	density					2236:2242	capillary density	2226:2242	capillary density	2226:2242	Although positive SDIR values - indicating interindividual differences in trainability - were obtained for aerobic capacity outcomes, maximal enzyme activities, capillary density, all fibre-specific outcomes and BNIP3 protein content, the remaining outcomes produced negative SDIR values indicating a large degree of random measurement error and/or within-subject variability.
33998072	6	134	theme	molecular	797:805	arg1	changes					807:813	morphological and molecular changes	779:813	morphological and molecular changes in human skeletal muscle	779:838	ABSTRACT Studies have interpreted a wide range of morphological and molecular changes in human skeletal muscle as evidence of interindividual differences in trainability.
33998072	7	135	theme	random	968:973	arg1	error					987:991	random measurement error	968:991	random measurement error	968:991	However, these interpretations fail to account for the influence of random measurement error and within-subject variability.
33998072	9	136	theme	control	1422:1428	arg1	4 weeks					1374:1380	4 weeks	1374:1380	4 weeks of continuous training (REL; n = 14) or control (n = 15)	1374:1437	Twenty-nine recreationally active males (age: 21 ± 2 years; BMI: 24 ± 3 kg/m2 ; ; 45 ± 7 ml/kg/min) completed 4 weeks of continuous training (REL; n = 14) or control (n = 15).
33998072	6	137	theme	morphological	779:791	arg1	changes					807:813	morphological and molecular changes	779:813	morphological and molecular changes in human skeletal muscle	779:838	ABSTRACT Studies have interpreted a wide range of morphological and molecular changes in human skeletal muscle as evidence of interindividual differences in trainability.
33998072	10	138	theme	BNIP3-like	1741:1750	arg1	markers					1683:1689	markers	1683:1689	markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1)	1683:1793	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	10	138	theme	BNIP3-like	1741:1750	arg1	protein					1752:1758	BNIP3-like protein	1741:1758	BNIP3-like protein	1741:1758	Maximal enzyme activities (citrate synthase and β-hydroxyacyl-CoA dehydrogenase), capillary density, fibre type composition, fibre-specific succinate dehydrogenase activity and substrate storage (intramuscular triglycerides and glycogen), and markers of mitophagy (BCL2-interacting protein 3 (BNIP3), BNIP3-like protein, parkin and PTEN-induced kinase 1) were measured in vastus lateralis samples collected before and after the intervention.
33998072	6	139	from	range	770:774	arg1	muscle					833:838	human skeletal muscle	818:838	human skeletal muscle	818:838	ABSTRACT Studies have interpreted a wide range of morphological and molecular changes in human skeletal muscle as evidence of interindividual differences in trainability.
33998072	12	140	theme	maximal	2199:2205	arg1	activities					2214:2223	maximal enzyme activities	2199:2223	maximal enzyme activities	2199:2223	Although positive SDIR values - indicating interindividual differences in trainability - were obtained for aerobic capacity outcomes, maximal enzyme activities, capillary density, all fibre-specific outcomes and BNIP3 protein content, the remaining outcomes produced negative SDIR values indicating a large degree of random measurement error and/or within-subject variability.
33998072	13	141	theme	exercise	2692:2699	arg1	training					2701:2708	exercise training	2692:2708	exercise training	2692:2708	Our findings question the interpretation of heterogeneity in observed responses as evidence of interindividual differences in trainability and highlight the importance of including control groups when analysing individual skeletal muscle response to exercise training.
34544271	9	0	theme	nonsterile	1857:1866	arg1	injury					1880:1885	nonsterile and sterile injury	1857:1885	nonsterile and sterile injury	1857:1885	These data identify an important vascular glycocalyx component that impacts hepatic failure during nonsterile and sterile injury.
34544271	9	1	theme	sterile	1872:1878	arg1	injury					1880:1885	nonsterile and sterile injury	1857:1885	nonsterile and sterile injury	1857:1885	These data identify an important vascular glycocalyx component that impacts hepatic failure during nonsterile and sterile injury.
34544271	2	2	theme	proteome	296:303	arg1	remodeling					305:314	rapid proteome remodeling	290:314	rapid proteome remodeling of the vascular surfaces	290:339	Using a chemical tagging workflow and high-resolution mass spectrometry, we demonstrate that rapid proteome remodeling of the vascular surfaces precedes hepatic damage in a murine model of Staphylococcus aureus sepsis.
34544271	2	3	theme	aureus	401:406	arg1	sepsis					408:413	Staphylococcus aureus sepsis	386:413	Staphylococcus aureus sepsis	386:413	Using a chemical tagging workflow and high-resolution mass spectrometry, we demonstrate that rapid proteome remodeling of the vascular surfaces precedes hepatic damage in a murine model of Staphylococcus aureus sepsis.
34544271	3	4	theme	factors	582:588	arg1	levels					540:545	altered levels	532:545	altered levels of heparin/heparan sulfate-binding factors	532:588	These early changes include vascular deposition of neutrophil-derived proteins, shedding of vascular receptors, and altered levels of heparin/heparan sulfate-binding factors.
34544271	3	4	theme	factors	582:588	arg1	shedding					496:503	shedding	496:503	shedding of vascular receptors	496:525	These early changes include vascular deposition of neutrophil-derived proteins, shedding of vascular receptors, and altered levels of heparin/heparan sulfate-binding factors.
34544271	3	4	theme	factors	582:588	arg1	deposition					453:462	vascular deposition	444:462	vascular deposition of neutrophil-derived proteins	444:493	These early changes include vascular deposition of neutrophil-derived proteins, shedding of vascular receptors, and altered levels of heparin/heparan sulfate-binding factors.
34544271	1	5	theme	septic	180:185	arg1	patients					187:194	septic patients	180:194	septic patients	180:194	Hepatic failure is an important risk factor for poor outcome in septic patients.
34544271	7	6	theme	liver	1295:1299	arg1	glycocalyx					1310:1319	the liver vascular glycocalyx	1291:1319	the liver vascular glycocalyx	1291:1319	This article shows that during Staphylococcus aureus sepsis, the liver vascular glycocalyx undergoes dramatic changes in protein composition associated with neutrophilic activity and heparin/heparan sulfate binding, all before organ damage is detectable by standard circulating liver damage markers or histology.
34544271	2	7	theme	vascular	323:330	arg1	surfaces					332:339	the vascular surfaces	319:339	the vascular surfaces	319:339	Using a chemical tagging workflow and high-resolution mass spectrometry, we demonstrate that rapid proteome remodeling of the vascular surfaces precedes hepatic damage in a murine model of Staphylococcus aureus sepsis.
34544271	6	8	theme	Vascular	1075:1082	arg1	remodeling					1095:1104	IMPORTANCE Vascular glycocalyx remodeling	1064:1104	IMPORTANCE Vascular glycocalyx remodeling	1064:1104	IMPORTANCE Vascular glycocalyx remodeling is critical to sepsis pathology, but the glycocalyx components that contribute to this process remain poorly characterized.
34544271	8	9	theme	sepsis-induced	1616:1629	arg1	hepatotoxicity					1631:1644	S. aureus sepsis-induced hepatotoxicity	1606:1644	S. aureus sepsis-induced hepatotoxicity	1606:1644	Targeted manipulation of endothelial heparan sulfate modulates S. aureus sepsis-induced hepatotoxicity by controlling the magnitude of neutrophilic infiltration into the liver in both nonsterile and sterile injury.
34544271	2	10	theme	high-resolution	235:249	arg1	spectrometry					256:267	high-resolution mass spectrometry	235:267	high-resolution mass spectrometry	235:267	Using a chemical tagging workflow and high-resolution mass spectrometry, we demonstrate that rapid proteome remodeling of the vascular surfaces precedes hepatic damage in a murine model of Staphylococcus aureus sepsis.
34544271	7	11	from	changes	1340:1346	arg1	composition					1359:1369	protein composition	1351:1369	protein composition	1351:1369	This article shows that during Staphylococcus aureus sepsis, the liver vascular glycocalyx undergoes dramatic changes in protein composition associated with neutrophilic activity and heparin/heparan sulfate binding, all before organ damage is detectable by standard circulating liver damage markers or histology.
34544271	8	12	dep	S.	1606:1607	arg1	aureus					1609:1614	aureus	1609:1614	aureus	1609:1614	Targeted manipulation of endothelial heparan sulfate modulates S. aureus sepsis-induced hepatotoxicity by controlling the magnitude of neutrophilic infiltration into the liver in both nonsterile and sterile injury.
34544271	7	13	theme	damage	1514:1519	arg1	markers					1521:1527	standard circulating liver damage markers	1487:1527	standard circulating liver damage markers	1487:1527	This article shows that during Staphylococcus aureus sepsis, the liver vascular glycocalyx undergoes dramatic changes in protein composition associated with neutrophilic activity and heparin/heparan sulfate binding, all before organ damage is detectable by standard circulating liver damage markers or histology.
34544271	1	14	theme	risk	148:151	arg1	factor					153:158	an important risk factor	135:158	an important risk factor for poor outcome in septic patients	135:194	Hepatic failure is an important risk factor for poor outcome in septic patients.
34544271	1	14	theme	risk	148:151	arg1	failure					124:130	Hepatic failure	116:130	Hepatic failure	116:130	Hepatic failure is an important risk factor for poor outcome in septic patients.
34544271	1	15	theme	important	138:146	arg1	factor					153:158	an important risk factor	135:158	an important risk factor for poor outcome in septic patients	135:194	Hepatic failure is an important risk factor for poor outcome in septic patients.
34544271	1	15	theme	important	138:146	arg1	failure					124:130	Hepatic failure	116:130	Hepatic failure	116:130	Hepatic failure is an important risk factor for poor outcome in septic patients.
34544271	7	16	theme	circulating	1496:1506	arg1	markers					1521:1527	standard circulating liver damage markers	1487:1527	standard circulating liver damage markers	1487:1527	This article shows that during Staphylococcus aureus sepsis, the liver vascular glycocalyx undergoes dramatic changes in protein composition associated with neutrophilic activity and heparin/heparan sulfate binding, all before organ damage is detectable by standard circulating liver damage markers or histology.
34544271	4	17	theme	hepatic	741:747	arg1	coagulopathy					749:760	hepatic coagulopathy	741:760	hepatic coagulopathy	741:760	Modification of endothelial heparan sulfate, a major component of the vascular glycocalyx, diminishes neutrophil trafficking to the liver and reduces hepatic coagulopathy and organ damage during the systemic inflammatory response to infection.
34544271	8	18	from	infiltration	1691:1702	arg1	injury					1750:1755	both nonsterile and sterile injury	1722:1755	both nonsterile and sterile injury	1722:1755	Targeted manipulation of endothelial heparan sulfate modulates S. aureus sepsis-induced hepatotoxicity by controlling the magnitude of neutrophilic infiltration into the liver in both nonsterile and sterile injury.
34544271	5	19	theme	heparan	974:980	arg1	sulfate					982:988	heparan sulfate	974:988	heparan sulfate contribution	974:1001	Modifying endothelial heparan sulfate likewise reduces neutrophil trafficking in sterile hepatic injury, reflecting a more general role of heparan sulfate contribution to the modulation of leukocyte behavior during inflammation.
34544271	6	20	theme	sepsis	1121:1126	arg1	pathology					1128:1136	sepsis pathology	1121:1136	sepsis pathology	1121:1136	IMPORTANCE Vascular glycocalyx remodeling is critical to sepsis pathology, but the glycocalyx components that contribute to this process remain poorly characterized.
34544271	8	21	theme	infiltration	1691:1702	arg1	magnitude					1665:1673	the magnitude	1661:1673	the magnitude of neutrophilic infiltration into the liver in both nonsterile and sterile injury	1661:1755	Targeted manipulation of endothelial heparan sulfate modulates S. aureus sepsis-induced hepatotoxicity by controlling the magnitude of neutrophilic infiltration into the liver in both nonsterile and sterile injury.
34544271	0	22	theme	Staphylococcus	86:99	arg1	Sepsis					108:113	Staphylococcus aureus Sepsis	86:113	Staphylococcus aureus Sepsis	86:113	Endothelial Heparan Sulfate Mediates Hepatic Neutrophil Trafficking and Injury during Staphylococcus aureus Sepsis.
34544271	5	23	theme	contribution	990:1001	arg1	role					966:969	a more general role	951:969	a more general role of heparan sulfate contribution to the modulation of leukocyte behavior during inflammation	951:1061	Modifying endothelial heparan sulfate likewise reduces neutrophil trafficking in sterile hepatic injury, reflecting a more general role of heparan sulfate contribution to the modulation of leukocyte behavior during inflammation.
34544271	5	24	theme	heparan	857:863	arg1	sulfate					865:871	endothelial heparan sulfate	845:871	endothelial heparan sulfate	845:871	Modifying endothelial heparan sulfate likewise reduces neutrophil trafficking in sterile hepatic injury, reflecting a more general role of heparan sulfate contribution to the modulation of leukocyte behavior during inflammation.
34544271	7	25	theme	standard	1487:1494	arg1	markers					1521:1527	standard circulating liver damage markers	1487:1527	standard circulating liver damage markers	1487:1527	This article shows that during Staphylococcus aureus sepsis, the liver vascular glycocalyx undergoes dramatic changes in protein composition associated with neutrophilic activity and heparin/heparan sulfate binding, all before organ damage is detectable by standard circulating liver damage markers or histology.
34544271	8	26	theme	nonsterile	1727:1736	arg1	injury					1750:1755	both nonsterile and sterile injury	1722:1755	both nonsterile and sterile injury	1722:1755	Targeted manipulation of endothelial heparan sulfate modulates S. aureus sepsis-induced hepatotoxicity by controlling the magnitude of neutrophilic infiltration into the liver in both nonsterile and sterile injury.
34544271	4	27	theme	inflammatory	799:810	arg1	response					812:819	the systemic inflammatory response	786:819	the systemic inflammatory response to infection	786:832	Modification of endothelial heparan sulfate, a major component of the vascular glycocalyx, diminishes neutrophil trafficking to the liver and reduces hepatic coagulopathy and organ damage during the systemic inflammatory response to infection.
34544271	3	28	theme	vascular	444:451	arg1	deposition					453:462	vascular deposition	444:462	vascular deposition of neutrophil-derived proteins	444:493	These early changes include vascular deposition of neutrophil-derived proteins, shedding of vascular receptors, and altered levels of heparin/heparan sulfate-binding factors.
34544271	0	29	theme	Endothelial	0:10	arg1	Sulfate					20:26	Endothelial Heparan Sulfate	0:26	Endothelial Heparan Sulfate	0:26	Endothelial Heparan Sulfate Mediates Hepatic Neutrophil Trafficking and Injury during Staphylococcus aureus Sepsis.
34544271	5	30	theme	behavior	1034:1041	arg1	modulation					1010:1019	the modulation	1006:1019	the modulation of leukocyte behavior during inflammation	1006:1061	Modifying endothelial heparan sulfate likewise reduces neutrophil trafficking in sterile hepatic injury, reflecting a more general role of heparan sulfate contribution to the modulation of leukocyte behavior during inflammation.
34544271	8	31	theme	sterile	1742:1748	arg1	injury					1750:1755	both nonsterile and sterile injury	1722:1755	both nonsterile and sterile injury	1722:1755	Targeted manipulation of endothelial heparan sulfate modulates S. aureus sepsis-induced hepatotoxicity by controlling the magnitude of neutrophilic infiltration into the liver in both nonsterile and sterile injury.
34544271	8	32	theme	Targeted	1543:1550	arg1	manipulation					1552:1563	Targeted manipulation	1543:1563	Targeted manipulation of endothelial heparan sulfate	1543:1594	Targeted manipulation of endothelial heparan sulfate modulates S. aureus sepsis-induced hepatotoxicity by controlling the magnitude of neutrophilic infiltration into the liver in both nonsterile and sterile injury.
34544271	5	33	theme	sterile	916:922	arg1	injury					932:937	sterile hepatic injury	916:937	sterile hepatic injury	916:937	Modifying endothelial heparan sulfate likewise reduces neutrophil trafficking in sterile hepatic injury, reflecting a more general role of heparan sulfate contribution to the modulation of leukocyte behavior during inflammation.
34544271	3	34	theme	sulfate-binding	566:580	arg1	factors					582:588	heparin/heparan sulfate-binding factors	550:588	heparin/heparan sulfate-binding factors	550:588	These early changes include vascular deposition of neutrophil-derived proteins, shedding of vascular receptors, and altered levels of heparin/heparan sulfate-binding factors.
34544271	9	35	theme	important	1781:1789	arg1	component					1811:1819	an important vascular glycocalyx component	1778:1819	an important vascular glycocalyx component that impacts hepatic failure during nonsterile and sterile injury	1778:1885	These data identify an important vascular glycocalyx component that impacts hepatic failure during nonsterile and sterile injury.
34544271	4	36	theme	heparan	619:625	arg1	component					644:652	a major component	636:652	a major component of the vascular glycocalyx	636:679	Modification of endothelial heparan sulfate, a major component of the vascular glycocalyx, diminishes neutrophil trafficking to the liver and reduces hepatic coagulopathy and organ damage during the systemic inflammatory response to infection.
34544271	4	36	theme	heparan	619:625	arg1	sulfate					627:633	endothelial heparan sulfate	607:633	endothelial heparan sulfate	607:633	Modification of endothelial heparan sulfate, a major component of the vascular glycocalyx, diminishes neutrophil trafficking to the liver and reduces hepatic coagulopathy and organ damage during the systemic inflammatory response to infection.
34544271	7	37	theme	vascular	1301:1308	arg1	glycocalyx					1310:1319	the liver vascular glycocalyx	1291:1319	the liver vascular glycocalyx	1291:1319	This article shows that during Staphylococcus aureus sepsis, the liver vascular glycocalyx undergoes dramatic changes in protein composition associated with neutrophilic activity and heparin/heparan sulfate binding, all before organ damage is detectable by standard circulating liver damage markers or histology.
34544271	3	38	theme	proteins	486:493	arg1	levels					540:545	altered levels	532:545	altered levels of heparin/heparan sulfate-binding factors	532:588	These early changes include vascular deposition of neutrophil-derived proteins, shedding of vascular receptors, and altered levels of heparin/heparan sulfate-binding factors.
34544271	3	38	theme	proteins	486:493	arg1	shedding					496:503	shedding	496:503	shedding of vascular receptors	496:525	These early changes include vascular deposition of neutrophil-derived proteins, shedding of vascular receptors, and altered levels of heparin/heparan sulfate-binding factors.
34544271	3	38	theme	proteins	486:493	arg1	deposition					453:462	vascular deposition	444:462	vascular deposition of neutrophil-derived proteins	444:493	These early changes include vascular deposition of neutrophil-derived proteins, shedding of vascular receptors, and altered levels of heparin/heparan sulfate-binding factors.
34544271	0	39	theme	Hepatic	37:43	arg1	Trafficking					56:66	Hepatic Neutrophil Trafficking	37:66	Hepatic Neutrophil Trafficking	37:66	Endothelial Heparan Sulfate Mediates Hepatic Neutrophil Trafficking and Injury during Staphylococcus aureus Sepsis.
34544271	9	40	theme	glycocalyx	1800:1809	arg1	component					1811:1819	an important vascular glycocalyx component	1778:1819	an important vascular glycocalyx component that impacts hepatic failure during nonsterile and sterile injury	1778:1885	These data identify an important vascular glycocalyx component that impacts hepatic failure during nonsterile and sterile injury.
34544271	8	41	theme	heparan	1580:1586	arg1	sulfate					1588:1594	endothelial heparan sulfate	1568:1594	endothelial heparan sulfate	1568:1594	Targeted manipulation of endothelial heparan sulfate modulates S. aureus sepsis-induced hepatotoxicity by controlling the magnitude of neutrophilic infiltration into the liver in both nonsterile and sterile injury.
34544271	2	42	theme	chemical	205:212	arg1	tagging					214:220	a chemical tagging workflow	203:229	a chemical tagging workflow	203:229	Using a chemical tagging workflow and high-resolution mass spectrometry, we demonstrate that rapid proteome remodeling of the vascular surfaces precedes hepatic damage in a murine model of Staphylococcus aureus sepsis.
34544271	3	43	theme	vascular	508:515	arg1	receptors					517:525	vascular receptors	508:525	vascular receptors	508:525	These early changes include vascular deposition of neutrophil-derived proteins, shedding of vascular receptors, and altered levels of heparin/heparan sulfate-binding factors.
34544271	9	44	theme	hepatic	1834:1840	arg1	failure					1842:1848	hepatic failure	1834:1848	hepatic failure	1834:1848	These data identify an important vascular glycocalyx component that impacts hepatic failure during nonsterile and sterile injury.
34544271	7	45	theme	sulfate	1429:1435	arg1	binding					1437:1443	heparin/heparan sulfate binding	1413:1443	heparin/heparan sulfate binding	1413:1443	This article shows that during Staphylococcus aureus sepsis, the liver vascular glycocalyx undergoes dramatic changes in protein composition associated with neutrophilic activity and heparin/heparan sulfate binding, all before organ damage is detectable by standard circulating liver damage markers or histology.
34544271	4	46	theme	vascular	661:668	arg1	glycocalyx					670:679	the vascular glycocalyx	657:679	the vascular glycocalyx	657:679	Modification of endothelial heparan sulfate, a major component of the vascular glycocalyx, diminishes neutrophil trafficking to the liver and reduces hepatic coagulopathy and organ damage during the systemic inflammatory response to infection.
34544271	2	47	theme	murine	370:375	arg1	model					377:381	a murine model	368:381	a murine model of Staphylococcus aureus sepsis	368:413	Using a chemical tagging workflow and high-resolution mass spectrometry, we demonstrate that rapid proteome remodeling of the vascular surfaces precedes hepatic damage in a murine model of Staphylococcus aureus sepsis.
34544271	7	48	theme	neutrophilic	1387:1398	arg1	activity					1400:1407	neutrophilic activity	1387:1407	neutrophilic activity	1387:1407	This article shows that during Staphylococcus aureus sepsis, the liver vascular glycocalyx undergoes dramatic changes in protein composition associated with neutrophilic activity and heparin/heparan sulfate binding, all before organ damage is detectable by standard circulating liver damage markers or histology.
34544271	8	49	from	injury	1750:1755	arg1	magnitude					1665:1673	the magnitude	1661:1673	the magnitude of neutrophilic infiltration into the liver in both nonsterile and sterile injury	1661:1755	Targeted manipulation of endothelial heparan sulfate modulates S. aureus sepsis-induced hepatotoxicity by controlling the magnitude of neutrophilic infiltration into the liver in both nonsterile and sterile injury.
34544271	5	50	theme	sulfate	982:988	arg1	contribution					990:1001	heparan sulfate contribution	974:1001	heparan sulfate contribution	974:1001	Modifying endothelial heparan sulfate likewise reduces neutrophil trafficking in sterile hepatic injury, reflecting a more general role of heparan sulfate contribution to the modulation of leukocyte behavior during inflammation.
34544271	4	51	theme	major	638:642	arg1	component					644:652	a major component	636:652	a major component of the vascular glycocalyx	636:679	Modification of endothelial heparan sulfate, a major component of the vascular glycocalyx, diminishes neutrophil trafficking to the liver and reduces hepatic coagulopathy and organ damage during the systemic inflammatory response to infection.
34544271	4	51	theme	major	638:642	arg1	sulfate					627:633	endothelial heparan sulfate	607:633	endothelial heparan sulfate	607:633	Modification of endothelial heparan sulfate, a major component of the vascular glycocalyx, diminishes neutrophil trafficking to the liver and reduces hepatic coagulopathy and organ damage during the systemic inflammatory response to infection.
34544271	2	52	theme	rapid	290:294	arg1	remodeling					305:314	rapid proteome remodeling	290:314	rapid proteome remodeling of the vascular surfaces	290:339	Using a chemical tagging workflow and high-resolution mass spectrometry, we demonstrate that rapid proteome remodeling of the vascular surfaces precedes hepatic damage in a murine model of Staphylococcus aureus sepsis.
34544271	5	53	theme	endothelial	845:855	arg1	sulfate					865:871	endothelial heparan sulfate	845:871	endothelial heparan sulfate	845:871	Modifying endothelial heparan sulfate likewise reduces neutrophil trafficking in sterile hepatic injury, reflecting a more general role of heparan sulfate contribution to the modulation of leukocyte behavior during inflammation.
34544271	7	54	theme	protein	1351:1357	arg1	composition					1359:1369	protein composition	1351:1369	protein composition	1351:1369	This article shows that during Staphylococcus aureus sepsis, the liver vascular glycocalyx undergoes dramatic changes in protein composition associated with neutrophilic activity and heparin/heparan sulfate binding, all before organ damage is detectable by standard circulating liver damage markers or histology.
34544271	2	55	theme	surfaces	332:339	arg1	remodeling					305:314	rapid proteome remodeling	290:314	rapid proteome remodeling of the vascular surfaces	290:339	Using a chemical tagging workflow and high-resolution mass spectrometry, we demonstrate that rapid proteome remodeling of the vascular surfaces precedes hepatic damage in a murine model of Staphylococcus aureus sepsis.
34544271	5	56	theme	general	958:964	arg1	role					966:969	a more general role	951:969	a more general role of heparan sulfate contribution to the modulation of leukocyte behavior during inflammation	951:1061	Modifying endothelial heparan sulfate likewise reduces neutrophil trafficking in sterile hepatic injury, reflecting a more general role of heparan sulfate contribution to the modulation of leukocyte behavior during inflammation.
34544271	6	57	theme	glycocalyx	1084:1093	arg1	remodeling					1095:1104	IMPORTANCE Vascular glycocalyx remodeling	1064:1104	IMPORTANCE Vascular glycocalyx remodeling	1064:1104	IMPORTANCE Vascular glycocalyx remodeling is critical to sepsis pathology, but the glycocalyx components that contribute to this process remain poorly characterized.
34544271	3	58	link	neutrophil-derived	467:484	arg1	proteins					486:493	neutrophil-derived proteins	467:493	neutrophil-derived proteins	467:493	These early changes include vascular deposition of neutrophil-derived proteins, shedding of vascular receptors, and altered levels of heparin/heparan sulfate-binding factors.
34544271	5	59	theme	hepatic	924:930	arg1	injury					932:937	sterile hepatic injury	916:937	sterile hepatic injury	916:937	Modifying endothelial heparan sulfate likewise reduces neutrophil trafficking in sterile hepatic injury, reflecting a more general role of heparan sulfate contribution to the modulation of leukocyte behavior during inflammation.
34544271	6	60	theme	IMPORTANCE	1064:1073	arg1	remodeling					1095:1104	IMPORTANCE Vascular glycocalyx remodeling	1064:1104	IMPORTANCE Vascular glycocalyx remodeling	1064:1104	IMPORTANCE Vascular glycocalyx remodeling is critical to sepsis pathology, but the glycocalyx components that contribute to this process remain poorly characterized.
34544271	3	61	theme	altered	532:538	arg1	levels					540:545	altered levels	532:545	altered levels of heparin/heparan sulfate-binding factors	532:588	These early changes include vascular deposition of neutrophil-derived proteins, shedding of vascular receptors, and altered levels of heparin/heparan sulfate-binding factors.
34544271	2	62	theme	mass	251:254	arg1	spectrometry					256:267	high-resolution mass spectrometry	235:267	high-resolution mass spectrometry	235:267	Using a chemical tagging workflow and high-resolution mass spectrometry, we demonstrate that rapid proteome remodeling of the vascular surfaces precedes hepatic damage in a murine model of Staphylococcus aureus sepsis.
34544271	4	63	theme	neutrophil	693:702	arg1	trafficking					704:714	neutrophil trafficking	693:714	neutrophil trafficking to the liver	693:727	Modification of endothelial heparan sulfate, a major component of the vascular glycocalyx, diminishes neutrophil trafficking to the liver and reduces hepatic coagulopathy and organ damage during the systemic inflammatory response to infection.
34544271	4	64	theme	organ	766:770	arg1	damage					772:777	organ damage	766:777	organ damage	766:777	Modification of endothelial heparan sulfate, a major component of the vascular glycocalyx, diminishes neutrophil trafficking to the liver and reduces hepatic coagulopathy and organ damage during the systemic inflammatory response to infection.
34544271	3	65	theme	heparin/heparan	550:564	arg1	factors					582:588	heparin/heparan sulfate-binding factors	550:588	heparin/heparan sulfate-binding factors	550:588	These early changes include vascular deposition of neutrophil-derived proteins, shedding of vascular receptors, and altered levels of heparin/heparan sulfate-binding factors.
34544271	0	66	theme	Neutrophil	45:54	arg1	Trafficking					56:66	Hepatic Neutrophil Trafficking	37:66	Hepatic Neutrophil Trafficking	37:66	Endothelial Heparan Sulfate Mediates Hepatic Neutrophil Trafficking and Injury during Staphylococcus aureus Sepsis.
34544271	8	67	theme	neutrophilic	1678:1689	arg1	infiltration					1691:1702	neutrophilic infiltration	1678:1702	neutrophilic infiltration into the liver in both nonsterile and sterile injury	1678:1755	Targeted manipulation of endothelial heparan sulfate modulates S. aureus sepsis-induced hepatotoxicity by controlling the magnitude of neutrophilic infiltration into the liver in both nonsterile and sterile injury.
34544271	2	68	theme	hepatic	350:356	arg1	damage					358:363	hepatic damage	350:363	hepatic damage	350:363	Using a chemical tagging workflow and high-resolution mass spectrometry, we demonstrate that rapid proteome remodeling of the vascular surfaces precedes hepatic damage in a murine model of Staphylococcus aureus sepsis.
34544271	7	69	theme	liver	1508:1512	arg1	markers					1521:1527	standard circulating liver damage markers	1487:1527	standard circulating liver damage markers	1487:1527	This article shows that during Staphylococcus aureus sepsis, the liver vascular glycocalyx undergoes dramatic changes in protein composition associated with neutrophilic activity and heparin/heparan sulfate binding, all before organ damage is detectable by standard circulating liver damage markers or histology.
34544271	0	70	theme	aureus	101:106	arg1	Sepsis					108:113	Staphylococcus aureus Sepsis	86:113	Staphylococcus aureus Sepsis	86:113	Endothelial Heparan Sulfate Mediates Hepatic Neutrophil Trafficking and Injury during Staphylococcus aureus Sepsis.
34544271	1	71	theme	poor	164:167	arg1	outcome					169:175	poor outcome	164:175	poor outcome in septic patients	164:194	Hepatic failure is an important risk factor for poor outcome in septic patients.
34544271	7	72	theme	dramatic	1331:1338	arg1	changes					1340:1346	dramatic changes	1331:1346	dramatic changes in protein composition associated with neutrophilic activity and heparin/heparan sulfate binding	1331:1443	This article shows that during Staphylococcus aureus sepsis, the liver vascular glycocalyx undergoes dramatic changes in protein composition associated with neutrophilic activity and heparin/heparan sulfate binding, all before organ damage is detectable by standard circulating liver damage markers or histology.
34544271	1	73	from	outcome	169:175	arg1	patients					187:194	septic patients	180:194	septic patients	180:194	Hepatic failure is an important risk factor for poor outcome in septic patients.
34544271	8	74	from	magnitude	1665:1673	arg1	injury					1750:1755	both nonsterile and sterile injury	1722:1755	both nonsterile and sterile injury	1722:1755	Targeted manipulation of endothelial heparan sulfate modulates S. aureus sepsis-induced hepatotoxicity by controlling the magnitude of neutrophilic infiltration into the liver in both nonsterile and sterile injury.
34544271	2	75	theme	sepsis	408:413	arg1	model					377:381	a murine model	368:381	a murine model of Staphylococcus aureus sepsis	368:413	Using a chemical tagging workflow and high-resolution mass spectrometry, we demonstrate that rapid proteome remodeling of the vascular surfaces precedes hepatic damage in a murine model of Staphylococcus aureus sepsis.
34544271	5	76	theme	neutrophil	890:899	arg1	trafficking					901:911	neutrophil trafficking	890:911	neutrophil trafficking in sterile hepatic injury	890:937	Modifying endothelial heparan sulfate likewise reduces neutrophil trafficking in sterile hepatic injury, reflecting a more general role of heparan sulfate contribution to the modulation of leukocyte behavior during inflammation.
34544271	5	77	from	trafficking	901:911	arg1	injury					932:937	sterile hepatic injury	916:937	sterile hepatic injury	916:937	Modifying endothelial heparan sulfate likewise reduces neutrophil trafficking in sterile hepatic injury, reflecting a more general role of heparan sulfate contribution to the modulation of leukocyte behavior during inflammation.
34544271	5	78	theme	leukocyte	1024:1032	arg1	behavior					1034:1041	leukocyte behavior	1024:1041	leukocyte behavior	1024:1041	Modifying endothelial heparan sulfate likewise reduces neutrophil trafficking in sterile hepatic injury, reflecting a more general role of heparan sulfate contribution to the modulation of leukocyte behavior during inflammation.
34544271	4	79	theme	endothelial	607:617	arg1	component					644:652	a major component	636:652	a major component of the vascular glycocalyx	636:679	Modification of endothelial heparan sulfate, a major component of the vascular glycocalyx, diminishes neutrophil trafficking to the liver and reduces hepatic coagulopathy and organ damage during the systemic inflammatory response to infection.
34544271	4	79	theme	endothelial	607:617	arg1	sulfate					627:633	endothelial heparan sulfate	607:633	endothelial heparan sulfate	607:633	Modification of endothelial heparan sulfate, a major component of the vascular glycocalyx, diminishes neutrophil trafficking to the liver and reduces hepatic coagulopathy and organ damage during the systemic inflammatory response to infection.
34544271	4	80	theme	systemic	790:797	arg1	response					812:819	the systemic inflammatory response	786:819	the systemic inflammatory response to infection	786:832	Modification of endothelial heparan sulfate, a major component of the vascular glycocalyx, diminishes neutrophil trafficking to the liver and reduces hepatic coagulopathy and organ damage during the systemic inflammatory response to infection.
34544271	2	81	theme	Staphylococcus	386:399	arg1	sepsis					408:413	Staphylococcus aureus sepsis	386:413	Staphylococcus aureus sepsis	386:413	Using a chemical tagging workflow and high-resolution mass spectrometry, we demonstrate that rapid proteome remodeling of the vascular surfaces precedes hepatic damage in a murine model of Staphylococcus aureus sepsis.
34544271	1	82	theme	Hepatic	116:122	arg1	factor					153:158	an important risk factor	135:158	an important risk factor for poor outcome in septic patients	135:194	Hepatic failure is an important risk factor for poor outcome in septic patients.
34544271	1	82	theme	Hepatic	116:122	arg1	failure					124:130	Hepatic failure	116:130	Hepatic failure	116:130	Hepatic failure is an important risk factor for poor outcome in septic patients.
34544271	7	83	theme	organ	1457:1461	arg1	damage					1463:1468	organ damage	1457:1468	organ damage	1457:1468	This article shows that during Staphylococcus aureus sepsis, the liver vascular glycocalyx undergoes dramatic changes in protein composition associated with neutrophilic activity and heparin/heparan sulfate binding, all before organ damage is detectable by standard circulating liver damage markers or histology.
34544271	4	84	theme	sulfate	627:633	arg1	Modification					591:602	Modification	591:602	Modification of endothelial heparan sulfate, a major component of the vascular glycocalyx,	591:680	Modification of endothelial heparan sulfate, a major component of the vascular glycocalyx, diminishes neutrophil trafficking to the liver and reduces hepatic coagulopathy and organ damage during the systemic inflammatory response to infection.
34544271	7	85	theme	aureus	1276:1281	arg1	sepsis					1283:1288	Staphylococcus aureus sepsis	1261:1288	Staphylococcus aureus sepsis	1261:1288	This article shows that during Staphylococcus aureus sepsis, the liver vascular glycocalyx undergoes dramatic changes in protein composition associated with neutrophilic activity and heparin/heparan sulfate binding, all before organ damage is detectable by standard circulating liver damage markers or histology.
34544271	9	86	theme	vascular	1791:1798	arg1	component					1811:1819	an important vascular glycocalyx component	1778:1819	an important vascular glycocalyx component that impacts hepatic failure during nonsterile and sterile injury	1778:1885	These data identify an important vascular glycocalyx component that impacts hepatic failure during nonsterile and sterile injury.
34544271	2	87	dep	tagging	214:220	arg1	workflow					222:229	workflow	222:229	workflow	222:229	Using a chemical tagging workflow and high-resolution mass spectrometry, we demonstrate that rapid proteome remodeling of the vascular surfaces precedes hepatic damage in a murine model of Staphylococcus aureus sepsis.
34544271	3	88	theme	early	422:426	arg1	changes					428:434	These early changes	416:434	These early changes	416:434	These early changes include vascular deposition of neutrophil-derived proteins, shedding of vascular receptors, and altered levels of heparin/heparan sulfate-binding factors.
34544271	8	89	theme	endothelial	1568:1578	arg1	sulfate					1588:1594	endothelial heparan sulfate	1568:1594	endothelial heparan sulfate	1568:1594	Targeted manipulation of endothelial heparan sulfate modulates S. aureus sepsis-induced hepatotoxicity by controlling the magnitude of neutrophilic infiltration into the liver in both nonsterile and sterile injury.
34544271	7	90	theme	heparin/heparan	1413:1427	arg1	sulfate					1429:1435	heparin/heparan sulfate	1413:1435	heparin/heparan sulfate binding	1413:1443	This article shows that during Staphylococcus aureus sepsis, the liver vascular glycocalyx undergoes dramatic changes in protein composition associated with neutrophilic activity and heparin/heparan sulfate binding, all before organ damage is detectable by standard circulating liver damage markers or histology.
34544271	8	91	theme	sulfate	1588:1594	arg1	manipulation					1552:1563	Targeted manipulation	1543:1563	Targeted manipulation of endothelial heparan sulfate	1543:1594	Targeted manipulation of endothelial heparan sulfate modulates S. aureus sepsis-induced hepatotoxicity by controlling the magnitude of neutrophilic infiltration into the liver in both nonsterile and sterile injury.
34544271	3	92	theme	receptors	517:525	arg1	levels					540:545	altered levels	532:545	altered levels of heparin/heparan sulfate-binding factors	532:588	These early changes include vascular deposition of neutrophil-derived proteins, shedding of vascular receptors, and altered levels of heparin/heparan sulfate-binding factors.
34544271	3	92	theme	receptors	517:525	arg1	shedding					496:503	shedding	496:503	shedding of vascular receptors	496:525	These early changes include vascular deposition of neutrophil-derived proteins, shedding of vascular receptors, and altered levels of heparin/heparan sulfate-binding factors.
34544271	3	92	theme	receptors	517:525	arg1	deposition					453:462	vascular deposition	444:462	vascular deposition of neutrophil-derived proteins	444:493	These early changes include vascular deposition of neutrophil-derived proteins, shedding of vascular receptors, and altered levels of heparin/heparan sulfate-binding factors.
34544271	3	93	theme	neutrophil-derived	467:484	arg1	proteins					486:493	neutrophil-derived proteins	467:493	neutrophil-derived proteins	467:493	These early changes include vascular deposition of neutrophil-derived proteins, shedding of vascular receptors, and altered levels of heparin/heparan sulfate-binding factors.
34544271	4	94	theme	glycocalyx	670:679	arg1	component					644:652	a major component	636:652	a major component of the vascular glycocalyx	636:679	Modification of endothelial heparan sulfate, a major component of the vascular glycocalyx, diminishes neutrophil trafficking to the liver and reduces hepatic coagulopathy and organ damage during the systemic inflammatory response to infection.
34544271	4	94	theme	glycocalyx	670:679	arg1	sulfate					627:633	endothelial heparan sulfate	607:633	endothelial heparan sulfate	607:633	Modification of endothelial heparan sulfate, a major component of the vascular glycocalyx, diminishes neutrophil trafficking to the liver and reduces hepatic coagulopathy and organ damage during the systemic inflammatory response to infection.
34544271	8	95	theme	S.	1606:1607	arg1	hepatotoxicity					1631:1644	S. aureus sepsis-induced hepatotoxicity	1606:1644	S. aureus sepsis-induced hepatotoxicity	1606:1644	Targeted manipulation of endothelial heparan sulfate modulates S. aureus sepsis-induced hepatotoxicity by controlling the magnitude of neutrophilic infiltration into the liver in both nonsterile and sterile injury.
34544271	0	96	theme	Heparan	12:18	arg1	Sulfate					20:26	Endothelial Heparan Sulfate	0:26	Endothelial Heparan Sulfate	0:26	Endothelial Heparan Sulfate Mediates Hepatic Neutrophil Trafficking and Injury during Staphylococcus aureus Sepsis.
33466460	3	0	from	dispersions	394:404	arg1	aerogels					373:380	aerogels	373:380	aerogels from gelled dispersions of CNFs and xyloglucan (XG)	373:432	Here, we prepared aerogels from gelled dispersions of CNFs and xyloglucan (XG) at different ratios by using a freeze-casting procedure in unidirectional (UD) and non-directional (ND) manners.
33466460	5	1	from	impact	647:652	arg1	properties					753:762	the water absorption properties	732:762	the water absorption properties	732:762	We investigated the impact of the freezing procedure and the gel's composition on the microstructure and the water absorption properties.
33466460	5	1	from	impact	647:652	arg1	microstructure					713:726	the microstructure	709:726	the microstructure	709:726	We investigated the impact of the freezing procedure and the gel's composition on the microstructure and the water absorption properties.
33466460	1	2	theme	inherent	167:174	arg1	properties					185:194	the inherent physical properties	163:194	the inherent physical properties of CNF	163:201	Bio-based aerogels containing cellulose nanofibrils (CNFs) are promising materials due to the inherent physical properties of CNF.
33466460	6	3	theme	XG	785:786	arg1	introduction					769:780	The introduction	765:780	The introduction of XG	765:786	The introduction of XG greatly affects the microstructure of the aerogel from lamellar to cellular morphology.
33466460	3	4	from	procedure	480:488	arg1	manners					538:544	unidirectional (UD) and non-directional (ND) manners	493:544	unidirectional (UD) and non-directional (ND) manners	493:544	Here, we prepared aerogels from gelled dispersions of CNFs and xyloglucan (XG) at different ratios by using a freeze-casting procedure in unidirectional (UD) and non-directional (ND) manners.
33466460	1	5	theme	physical	176:183	arg1	properties					185:194	the inherent physical properties	163:194	the inherent physical properties of CNF	163:201	Bio-based aerogels containing cellulose nanofibrils (CNFs) are promising materials due to the inherent physical properties of CNF.
33466460	6	6	theme	cellular	855:862	arg1	morphology					864:873	cellular morphology	855:873	cellular morphology	855:873	The introduction of XG greatly affects the microstructure of the aerogel from lamellar to cellular morphology.
33466460	7	7	theme	shape	938:942	arg1	recovery					944:951	shape recovery	938:951	shape recovery after compression	938:969	Bio-based aerogels showed high water absorption capacity with shape recovery after compression.
33466460	1	8	theme	Bio-based	73:81	arg1	materials					146:154	promising materials	136:154	promising materials due to the inherent physical properties of CNF	136:201	Bio-based aerogels containing cellulose nanofibrils (CNFs) are promising materials due to the inherent physical properties of CNF.
33466460	1	8	theme	Bio-based	73:81	arg1	aerogels					83:90	Bio-based aerogels	73:90	Bio-based aerogels containing cellulose nanofibrils (CNFs)	73:130	Bio-based aerogels containing cellulose nanofibrils (CNFs) are promising materials due to the inherent physical properties of CNF.
33466460	7	9	theme	absorption	913:922	arg1	capacity					924:931	high water absorption capacity	902:931	high water absorption capacity	902:931	Bio-based aerogels showed high water absorption capacity with shape recovery after compression.
33466460	3	10	theme	non-directional	517:531	arg1	manners					538:544	unidirectional (UD) and non-directional (ND) manners	493:544	unidirectional (UD) and non-directional (ND) manners	493:544	Here, we prepared aerogels from gelled dispersions of CNFs and xyloglucan (XG) at different ratios by using a freeze-casting procedure in unidirectional (UD) and non-directional (ND) manners.
33466460	8	11	dep	morphology	993:1002	arg1	compositions					1016:1027	compositions	1016:1027	compositions	1016:1027	The relation between morphology and aerogel compositions is discussed.
33466460	3	12	dep	non-directional	517:531	arg1	ND					534:535	ND	534:535	ND	534:535	Here, we prepared aerogels from gelled dispersions of CNFs and xyloglucan (XG) at different ratios by using a freeze-casting procedure in unidirectional (UD) and non-directional (ND) manners.
33466460	4	13	theme	true	616:619	arg1	gels					621:624	true gels	616:624	true gels	616:624	As showed by rheology analysis, CNF and CNF/XG dispersions behave as true gels.
33466460	6	14	from	lamellar	843:850	arg1	microstructure					808:821	the microstructure	804:821	the microstructure of the aerogel from lamellar to cellular morphology	804:873	The introduction of XG greatly affects the microstructure of the aerogel from lamellar to cellular morphology.
33466460	6	14	from	lamellar	843:850	arg1	aerogel					830:836	the aerogel	826:836	the aerogel from lamellar to cellular morphology	826:873	The introduction of XG greatly affects the microstructure of the aerogel from lamellar to cellular morphology.
33466460	7	15	theme	high	902:905	arg1	capacity					924:931	high water absorption capacity	902:931	high water absorption capacity	902:931	Bio-based aerogels showed high water absorption capacity with shape recovery after compression.
33466460	1	16	theme	cellulose	103:111	arg1	CNFs					126:129	CNFs	126:129	CNFs	126:129	Bio-based aerogels containing cellulose nanofibrils (CNFs) are promising materials due to the inherent physical properties of CNF.
33466460	1	16	theme	cellulose	103:111	arg1	nanofibrils					113:123	cellulose nanofibrils	103:123	cellulose nanofibrils (CNFs)	103:130	Bio-based aerogels containing cellulose nanofibrils (CNFs) are promising materials due to the inherent physical properties of CNF.
33466460	6	17	from	microstructure	808:821	arg1	lamellar					843:850	lamellar	843:850	lamellar	843:850	The introduction of XG greatly affects the microstructure of the aerogel from lamellar to cellular morphology.
33466460	6	18	theme	aerogel	830:836	arg1	microstructure					808:821	the microstructure	804:821	the microstructure of the aerogel from lamellar to cellular morphology	804:873	The introduction of XG greatly affects the microstructure of the aerogel from lamellar to cellular morphology.
33466460	7	19	theme	water	907:911	arg1	capacity					924:931	high water absorption capacity	902:931	high water absorption capacity	902:931	Bio-based aerogels showed high water absorption capacity with shape recovery after compression.
33466460	1	20	theme	CNF	199:201	arg1	properties					185:194	the inherent physical properties	163:194	the inherent physical properties of CNF	163:201	Bio-based aerogels containing cellulose nanofibrils (CNFs) are promising materials due to the inherent physical properties of CNF.
33466460	3	21	theme	xyloglucan	418:427	arg1	dispersions					394:404	gelled dispersions	387:404	gelled dispersions of CNFs and xyloglucan (XG)	387:432	Here, we prepared aerogels from gelled dispersions of CNFs and xyloglucan (XG) at different ratios by using a freeze-casting procedure in unidirectional (UD) and non-directional (ND) manners.
33466460	1	22	contain	containing	92:101	arg1	materials					146:154	promising materials	136:154	promising materials due to the inherent physical properties of CNF	136:201	Bio-based aerogels containing cellulose nanofibrils (CNFs) are promising materials due to the inherent physical properties of CNF.
33466460	1	22	contain	containing	92:101	arg2	CNFs					126:129	CNFs	126:129	CNFs	126:129	Bio-based aerogels containing cellulose nanofibrils (CNFs) are promising materials due to the inherent physical properties of CNF.
33466460	1	22	contain	containing	92:101	arg1	aerogels					83:90	Bio-based aerogels	73:90	Bio-based aerogels containing cellulose nanofibrils (CNFs)	73:130	Bio-based aerogels containing cellulose nanofibrils (CNFs) are promising materials due to the inherent physical properties of CNF.
33466460	1	22	contain	containing	92:101	arg2	nanofibrils					113:123	cellulose nanofibrils	103:123	cellulose nanofibrils (CNFs)	103:130	Bio-based aerogels containing cellulose nanofibrils (CNFs) are promising materials due to the inherent physical properties of CNF.
33466460	3	23	dep	unidirectional	493:506	arg1	UD					509:510	UD	509:510	UD	509:510	Here, we prepared aerogels from gelled dispersions of CNFs and xyloglucan (XG) at different ratios by using a freeze-casting procedure in unidirectional (UD) and non-directional (ND) manners.
33466460	3	24	theme	different	437:445	arg1	ratios					447:452	different ratios	437:452	different ratios	437:452	Here, we prepared aerogels from gelled dispersions of CNFs and xyloglucan (XG) at different ratios by using a freeze-casting procedure in unidirectional (UD) and non-directional (ND) manners.
33466460	2	25	with	biomaterials	321:332	arg1	properties					343:352	new properties	339:352	new properties	339:352	The high affinity of cellulose to plant hemicelluloses (xyloglucan, xylan, pectin) is also an opportunity to develop biomaterials with new properties.
33466460	0	26	theme	Shape	57:61	arg1	Recovery					63:70	Shape Recovery	57:70	Shape Recovery	57:70	Cellulose Nanofibrils/Xyloglucan Bio-Based Aerogels with Shape Recovery.
33466460	3	27	theme	freeze-casting	465:478	arg1	procedure					480:488	a freeze-casting procedure	463:488	a freeze-casting procedure in unidirectional (UD) and non-directional (ND) manners	463:544	Here, we prepared aerogels from gelled dispersions of CNFs and xyloglucan (XG) at different ratios by using a freeze-casting procedure in unidirectional (UD) and non-directional (ND) manners.
33466460	5	28	theme	freezing	661:668	arg1	procedure					670:678	the freezing procedure	657:678	the freezing procedure	657:678	We investigated the impact of the freezing procedure and the gel's composition on the microstructure and the water absorption properties.
33466460	2	29	dep	hemicelluloses	244:257	arg1	xyloglucan					260:269	xyloglucan	260:269	xyloglucan	260:269	The high affinity of cellulose to plant hemicelluloses (xyloglucan, xylan, pectin) is also an opportunity to develop biomaterials with new properties.
33466460	2	29	dep	hemicelluloses	244:257	arg1	pectin					279:284	pectin	279:284	pectin	279:284	The high affinity of cellulose to plant hemicelluloses (xyloglucan, xylan, pectin) is also an opportunity to develop biomaterials with new properties.
33466460	2	29	dep	hemicelluloses	244:257	arg1	xylan					272:276	xylan	272:276	xylan	272:276	The high affinity of cellulose to plant hemicelluloses (xyloglucan, xylan, pectin) is also an opportunity to develop biomaterials with new properties.
33466460	3	30	theme	CNFs	409:412	arg1	dispersions					394:404	gelled dispersions	387:404	gelled dispersions of CNFs and xyloglucan (XG)	387:432	Here, we prepared aerogels from gelled dispersions of CNFs and xyloglucan (XG) at different ratios by using a freeze-casting procedure in unidirectional (UD) and non-directional (ND) manners.
33466460	5	31	theme	procedure	670:678	arg1	impact					647:652	the impact	643:652	the impact of the freezing procedure and the gel's composition on the microstructure and the water absorption properties	643:762	We investigated the impact of the freezing procedure and the gel's composition on the microstructure and the water absorption properties.
33466460	2	32	theme	plant	238:242	arg1	hemicelluloses					244:257	plant hemicelluloses	238:257	plant hemicelluloses (xyloglucan, xylan, pectin)	238:285	The high affinity of cellulose to plant hemicelluloses (xyloglucan, xylan, pectin) is also an opportunity to develop biomaterials with new properties.
33466460	3	33	theme	gelled	387:392	arg1	dispersions					394:404	gelled dispersions	387:404	gelled dispersions of CNFs and xyloglucan (XG)	387:432	Here, we prepared aerogels from gelled dispersions of CNFs and xyloglucan (XG) at different ratios by using a freeze-casting procedure in unidirectional (UD) and non-directional (ND) manners.
33466460	2	34	theme	new	339:341	arg1	properties					343:352	new properties	339:352	new properties	339:352	The high affinity of cellulose to plant hemicelluloses (xyloglucan, xylan, pectin) is also an opportunity to develop biomaterials with new properties.
33466460	5	35	theme	absorption	742:751	arg1	properties					753:762	the water absorption properties	732:762	the water absorption properties	732:762	We investigated the impact of the freezing procedure and the gel's composition on the microstructure and the water absorption properties.
33466460	2	36	theme	cellulose	225:233	arg1	opportunity					298:308	an opportunity	295:308	an opportunity to develop biomaterials with new properties	295:352	The high affinity of cellulose to plant hemicelluloses (xyloglucan, xylan, pectin) is also an opportunity to develop biomaterials with new properties.
33466460	2	36	theme	cellulose	225:233	arg1	affinity					213:220	The high affinity	204:220	The high affinity of cellulose to plant hemicelluloses (xyloglucan, xylan, pectin)	204:285	The high affinity of cellulose to plant hemicelluloses (xyloglucan, xylan, pectin) is also an opportunity to develop biomaterials with new properties.
33466460	1	37	theme	promising	136:144	arg1	materials					146:154	promising materials	136:154	promising materials due to the inherent physical properties of CNF	136:201	Bio-based aerogels containing cellulose nanofibrils (CNFs) are promising materials due to the inherent physical properties of CNF.
33466460	1	37	theme	promising	136:144	arg1	aerogels					83:90	Bio-based aerogels	73:90	Bio-based aerogels containing cellulose nanofibrils (CNFs)	73:130	Bio-based aerogels containing cellulose nanofibrils (CNFs) are promising materials due to the inherent physical properties of CNF.
33466460	7	38	theme	Bio-based	876:884	arg1	aerogels					886:893	Bio-based aerogels	876:893	Bio-based aerogels	876:893	Bio-based aerogels showed high water absorption capacity with shape recovery after compression.
33466460	2	39	theme	high	208:211	arg1	opportunity					298:308	an opportunity	295:308	an opportunity to develop biomaterials with new properties	295:352	The high affinity of cellulose to plant hemicelluloses (xyloglucan, xylan, pectin) is also an opportunity to develop biomaterials with new properties.
33466460	2	39	theme	high	208:211	arg1	affinity					213:220	The high affinity	204:220	The high affinity of cellulose to plant hemicelluloses (xyloglucan, xylan, pectin)	204:285	The high affinity of cellulose to plant hemicelluloses (xyloglucan, xylan, pectin) is also an opportunity to develop biomaterials with new properties.
33466460	1	40	theme	due	156:158	arg1	materials					146:154	promising materials	136:154	promising materials due to the inherent physical properties of CNF	136:201	Bio-based aerogels containing cellulose nanofibrils (CNFs) are promising materials due to the inherent physical properties of CNF.
33466460	1	40	theme	due	156:158	arg1	aerogels					83:90	Bio-based aerogels	73:90	Bio-based aerogels containing cellulose nanofibrils (CNFs)	73:130	Bio-based aerogels containing cellulose nanofibrils (CNFs) are promising materials due to the inherent physical properties of CNF.
33466460	4	41	theme	rheology	560:567	arg1	analysis					569:576	rheology analysis	560:576	rheology analysis	560:576	As showed by rheology analysis, CNF and CNF/XG dispersions behave as true gels.
33466460	5	42	theme	composition	694:704	arg1	impact					647:652	the impact	643:652	the impact of the freezing procedure and the gel's composition on the microstructure and the water absorption properties	643:762	We investigated the impact of the freezing procedure and the gel's composition on the microstructure and the water absorption properties.
33466460	4	43	theme	CNF/XG	587:592	arg1	dispersions					594:604	CNF and CNF/XG dispersions	579:604	CNF and CNF/XG dispersions	579:604	As showed by rheology analysis, CNF and CNF/XG dispersions behave as true gels.
33466460	3	44	theme	unidirectional	493:506	arg1	manners					538:544	unidirectional (UD) and non-directional (ND) manners	493:544	unidirectional (UD) and non-directional (ND) manners	493:544	Here, we prepared aerogels from gelled dispersions of CNFs and xyloglucan (XG) at different ratios by using a freeze-casting procedure in unidirectional (UD) and non-directional (ND) manners.
33466460	4	45	theme	CNF	579:581	arg1	dispersions					594:604	CNF and CNF/XG dispersions	579:604	CNF and CNF/XG dispersions	579:604	As showed by rheology analysis, CNF and CNF/XG dispersions behave as true gels.
33466460	5	46	theme	water	736:740	arg1	properties					753:762	the water absorption properties	732:762	the water absorption properties	732:762	We investigated the impact of the freezing procedure and the gel's composition on the microstructure and the water absorption properties.
32714354	9	0	theme	cell	1577:1580	arg1	walls					1582:1586	cell walls	1577:1586	cell walls	1577:1586	Side chain analyses and molecular weight elution profiles of polyuronides and xyloglucans of cell wall extracts revealed fundamental differences between the genotypes, pointing towards a weakening of the structural integrity of cell walls in the fast softening 'AC-F' genotype even at the firm, unripe stage.
32714354	11	1	from	differences	2014:2024	arg1	structure					2043:2051	the cell wall structure	2029:2051	the cell wall structure	2029:2051	Together these results suggest that the different rates of softening between 'AC-F' and 'AC-S' are not due to changes in enzyme activities alone, but that fundamental differences in the cell wall structure are likely to influence the rates of softening through differential modification and accessibility of specific cell wall polysaccharides during ripening.
32714354	1	2	theme	wall-associated	238:252	arg1	enzymes					254:260	cell wall-associated enzymes	233:260	cell wall-associated enzymes	233:260	Fruit softening is controlled by hormonal and developmental cues, causing an upregulation of cell wall-associated enzymes that break down the complex sugar matrices in the cell wall.
32714354	11	3	theme	polysaccharides	2174:2188	arg1	accessibility					2138:2150	accessibility	2138:2150	accessibility	2138:2150	Together these results suggest that the different rates of softening between 'AC-F' and 'AC-S' are not due to changes in enzyme activities alone, but that fundamental differences in the cell wall structure are likely to influence the rates of softening through differential modification and accessibility of specific cell wall polysaccharides during ripening.
32714354	11	3	theme	polysaccharides	2174:2188	arg1	modification					2121:2132	differential modification	2108:2132	differential modification	2108:2132	Together these results suggest that the different rates of softening between 'AC-F' and 'AC-S' are not due to changes in enzyme activities alone, but that fundamental differences in the cell wall structure are likely to influence the rates of softening through differential modification and accessibility of specific cell wall polysaccharides during ripening.
32714354	1	4	from	matrices	296:303	arg1	wall					317:320	the cell wall	308:320	the cell wall	308:320	Fruit softening is controlled by hormonal and developmental cues, causing an upregulation of cell wall-associated enzymes that break down the complex sugar matrices in the cell wall.
32714354	9	5	theme	fast	1595:1598	arg1	genotype					1617:1624	the fast softening 'AC-F' genotype	1591:1624	the fast softening 'AC-F' genotype	1591:1624	Side chain analyses and molecular weight elution profiles of polyuronides and xyloglucans of cell wall extracts revealed fundamental differences between the genotypes, pointing towards a weakening of the structural integrity of cell walls in the fast softening 'AC-F' genotype even at the firm, unripe stage.
32714354	11	6	theme	softening	2090:2098	arg1	rates					2081:2085	the rates	2077:2085	the rates of softening through differential modification and accessibility of specific cell wall polysaccharides during ripening	2077:2204	Together these results suggest that the different rates of softening between 'AC-F' and 'AC-S' are not due to changes in enzyme activities alone, but that fundamental differences in the cell wall structure are likely to influence the rates of softening through differential modification and accessibility of specific cell wall polysaccharides during ripening.
32714354	9	7	theme	chain	1354:1358	arg1	analyses					1360:1367	Side chain analyses	1349:1367	Side chain analyses	1349:1367	Side chain analyses and molecular weight elution profiles of polyuronides and xyloglucans of cell wall extracts revealed fundamental differences between the genotypes, pointing towards a weakening of the structural integrity of cell walls in the fast softening 'AC-F' genotype even at the firm, unripe stage.
32714354	4	8	theme	Actinidia	635:643	arg1	var					655:657	two Actinidia chinensis var	631:657	two Actinidia chinensis var	631:657	To address this question, the softening of two Actinidia chinensis var.
32714354	1	9	theme	hormonal	173:180	arg1	cues					200:203	hormonal and developmental cues	173:203	hormonal and developmental cues	173:203	Fruit softening is controlled by hormonal and developmental cues, causing an upregulation of cell wall-associated enzymes that break down the complex sugar matrices in the cell wall.
32714354	8	10	theme	pectin	1191:1196	arg1	domain					1198:1203	the pectin domain	1187:1203	the pectin domain	1187:1203	In the pectin domain, differences in pectin solubilization and location of methyl-esterified homogalacturonan in the cell wall between 'AC-S' and 'AC-F' were shown.
32714354	7	11	theme	xyloglucanase	995:1007	arg1	activity					1016:1023	xyloglucanase enzyme activity	995:1023	xyloglucanase enzyme activity	995:1023	In the hemicellulose domain, xyloglucanase enzyme activity was higher in 'AC-F' at the firm unripe stage, a finding supported by differential expression of xyloglucan transglycosylase/hydrolase genes during softening.
32714354	3	12	theme	wall	540:543	arg1	structure					545:553	cell wall structure	535:553	cell wall structure	535:553	Currently, little is known about the relationship between cell wall structure and the rate of fruit softening.
32714354	1	13	theme	developmental	186:198	arg1	cues					200:203	hormonal and developmental cues	173:203	hormonal and developmental cues	173:203	Fruit softening is controlled by hormonal and developmental cues, causing an upregulation of cell wall-associated enzymes that break down the complex sugar matrices in the cell wall.
32714354	6	14	theme	wall	872:875	arg1	structure					877:885	the cell wall structure	863:885	the cell wall structure of the two genotypes	863:906	Throughout softening, the cell wall structure of the two genotypes was fundamentally different at identical firmness stages.
32714354	6	14	theme	wall	872:875	arg1	different					926:934	different	926:934	different	926:934	Throughout softening, the cell wall structure of the two genotypes was fundamentally different at identical firmness stages.
32714354	11	15	theme	softening	1906:1914	arg1	due					1950:1952	due	1950:1952	due	1950:1952	Together these results suggest that the different rates of softening between 'AC-F' and 'AC-S' are not due to changes in enzyme activities alone, but that fundamental differences in the cell wall structure are likely to influence the rates of softening through differential modification and accessibility of specific cell wall polysaccharides during ripening.
32714354	11	15	theme	softening	1906:1914	arg1	rates					1897:1901	the different rates	1883:1901	the different rates of softening between 'AC-F' and 'AC-S'	1883:1940	Together these results suggest that the different rates of softening between 'AC-F' and 'AC-S' are not due to changes in enzyme activities alone, but that fundamental differences in the cell wall structure are likely to influence the rates of softening through differential modification and accessibility of specific cell wall polysaccharides during ripening.
32714354	0	16	from	Differences	73:83	arg1	Domains					114:120	the Xyloglucan and Pectin Domains	88:120	Domains	114:120	Fruit From Two Kiwifruit Genotypes With Contrasting Softening Rates Show Differences in the Xyloglucan and Pectin Domains of the Cell Wall.
32714354	0	16	from	Differences	73:83	arg1	Wall					134:137	the Cell Wall	125:137	the Cell Wall	125:137	Fruit From Two Kiwifruit Genotypes With Contrasting Softening Rates Show Differences in the Xyloglucan and Pectin Domains of the Cell Wall.
32714354	11	17	theme	differential	2108:2119	arg1	modification					2121:2132	differential modification	2108:2132	differential modification	2108:2132	Together these results suggest that the different rates of softening between 'AC-F' and 'AC-S' are not due to changes in enzyme activities alone, but that fundamental differences in the cell wall structure are likely to influence the rates of softening through differential modification and accessibility of specific cell wall polysaccharides during ripening.
32714354	3	18	theme	fruit	571:575	arg1	softening					577:585	fruit softening	571:585	fruit softening	571:585	Currently, little is known about the relationship between cell wall structure and the rate of fruit softening.
32714354	10	19	from	AC-S	1809:1812	arg1	easier					1731:1736	easier	1731:1736	easier	1731:1736	As a consequence, the polysaccharides in the cell walls of 'AC-F' may be easier to access and hence more susceptible to enzymatic degradation than in 'AC-S', resulting in faster softening.
32714354	10	19	from	AC-S	1809:1812	arg1	polysaccharides					1680:1694	the polysaccharides	1676:1694	the polysaccharides in the cell walls of 'AC-F'	1676:1722	As a consequence, the polysaccharides in the cell walls of 'AC-F' may be easier to access and hence more susceptible to enzymatic degradation than in 'AC-S', resulting in faster softening.
32714354	0	20	theme	Wall	134:137	arg1	Domains					114:120	the Xyloglucan and Pectin Domains	88:120	Domains	114:120	Fruit From Two Kiwifruit Genotypes With Contrasting Softening Rates Show Differences in the Xyloglucan and Pectin Domains of the Cell Wall.
32714354	0	20	theme	Wall	134:137	arg1	Wall					134:137	the Cell Wall	125:137	the Cell Wall	125:137	Fruit From Two Kiwifruit Genotypes With Contrasting Softening Rates Show Differences in the Xyloglucan and Pectin Domains of the Cell Wall.
32714354	4	21	dep	address	591:597	arg1	softening					618:626	the softening	614:626	the softening of two Actinidia chinensis var	614:657	To address this question, the softening of two Actinidia chinensis var.
32714354	5	22	theme	techniques	829:838	arg1	range					766:770	a range	764:770	a range of compositional, biochemical, structural, and molecular techniques	764:838	chinensis (kiwifruit) genotypes (a fast 'AC-F' and a slow 'AC-S' softening genotype) was examined using a range of compositional, biochemical, structural, and molecular techniques.
32714354	5	23	theme	chinensis	660:668	arg1	genotype					735:742	a slow 'AC-S' softening genotype	711:742	a slow 'AC-S' softening genotype	711:742	chinensis (kiwifruit) genotypes (a fast 'AC-F' and a slow 'AC-S' softening genotype) was examined using a range of compositional, biochemical, structural, and molecular techniques.
32714354	5	23	theme	chinensis	660:668	arg1	AC-F					701:704	a fast 'AC-F'	693:705	a fast 'AC-F'	693:705	chinensis (kiwifruit) genotypes (a fast 'AC-F' and a slow 'AC-S' softening genotype) was examined using a range of compositional, biochemical, structural, and molecular techniques.
32714354	5	23	theme	chinensis	660:668	arg1	genotypes					682:690	chinensis (kiwifruit) genotypes	660:690	chinensis (kiwifruit) genotypes (a fast 'AC-F' and a slow 'AC-S' softening genotype)	660:743	chinensis (kiwifruit) genotypes (a fast 'AC-F' and a slow 'AC-S' softening genotype) was examined using a range of compositional, biochemical, structural, and molecular techniques.
32714354	10	24	theme	AC-F	1718:1721	arg1	walls					1708:1712	the cell walls	1699:1712	the cell walls of 'AC-F'	1699:1722	As a consequence, the polysaccharides in the cell walls of 'AC-F' may be easier to access and hence more susceptible to enzymatic degradation than in 'AC-S', resulting in faster softening.
32714354	9	25	theme	xyloglucans	1427:1437	arg1	analyses					1360:1367	Side chain analyses	1349:1367	Side chain analyses	1349:1367	Side chain analyses and molecular weight elution profiles of polyuronides and xyloglucans of cell wall extracts revealed fundamental differences between the genotypes, pointing towards a weakening of the structural integrity of cell walls in the fast softening 'AC-F' genotype even at the firm, unripe stage.
32714354	9	25	theme	xyloglucans	1427:1437	arg1	profiles					1398:1405	molecular weight elution profiles	1373:1405	molecular weight elution profiles	1373:1405	Side chain analyses and molecular weight elution profiles of polyuronides and xyloglucans of cell wall extracts revealed fundamental differences between the genotypes, pointing towards a weakening of the structural integrity of cell walls in the fast softening 'AC-F' genotype even at the firm, unripe stage.
32714354	6	26	theme	identical	939:947	arg1	stages					958:963	identical firmness stages	939:963	identical firmness stages	939:963	Throughout softening, the cell wall structure of the two genotypes was fundamentally different at identical firmness stages.
32714354	11	27	theme	wall	2038:2041	arg1	structure					2043:2051	the cell wall structure	2029:2051	the cell wall structure	2029:2051	Together these results suggest that the different rates of softening between 'AC-F' and 'AC-S' are not due to changes in enzyme activities alone, but that fundamental differences in the cell wall structure are likely to influence the rates of softening through differential modification and accessibility of specific cell wall polysaccharides during ripening.
32714354	4	28	theme	var	655:657	arg1	softening					618:626	the softening	614:626	the softening of two Actinidia chinensis var	614:657	To address this question, the softening of two Actinidia chinensis var.
32714354	7	29	theme	genes	1160:1164	arg1	expression					1108:1117	differential expression	1095:1117	differential expression of xyloglucan transglycosylase/hydrolase genes during softening	1095:1181	In the hemicellulose domain, xyloglucanase enzyme activity was higher in 'AC-F' at the firm unripe stage, a finding supported by differential expression of xyloglucan transglycosylase/hydrolase genes during softening.
32714354	1	30	theme	cell	312:315	arg1	wall					317:320	the cell wall	308:320	the cell wall	308:320	Fruit softening is controlled by hormonal and developmental cues, causing an upregulation of cell wall-associated enzymes that break down the complex sugar matrices in the cell wall.
32714354	9	31	theme	cell	1442:1445	arg1	extracts					1452:1459	cell wall extracts	1442:1459	cell wall extracts	1442:1459	Side chain analyses and molecular weight elution profiles of polyuronides and xyloglucans of cell wall extracts revealed fundamental differences between the genotypes, pointing towards a weakening of the structural integrity of cell walls in the fast softening 'AC-F' genotype even at the firm, unripe stage.
32714354	8	32	theme	methyl-esterified	1259:1275	arg1	homogalacturonan					1277:1292	methyl-esterified homogalacturonan	1259:1292	methyl-esterified homogalacturonan	1259:1292	In the pectin domain, differences in pectin solubilization and location of methyl-esterified homogalacturonan in the cell wall between 'AC-S' and 'AC-F' were shown.
32714354	7	33	theme	xyloglucan	1122:1131	arg1	genes					1160:1164	xyloglucan transglycosylase/hydrolase genes	1122:1164	xyloglucan transglycosylase/hydrolase genes	1122:1164	In the hemicellulose domain, xyloglucanase enzyme activity was higher in 'AC-F' at the firm unripe stage, a finding supported by differential expression of xyloglucan transglycosylase/hydrolase genes during softening.
32714354	9	34	theme	extracts	1452:1459	arg1	xyloglucans					1427:1437	xyloglucans	1427:1437	xyloglucans	1427:1437	Side chain analyses and molecular weight elution profiles of polyuronides and xyloglucans of cell wall extracts revealed fundamental differences between the genotypes, pointing towards a weakening of the structural integrity of cell walls in the fast softening 'AC-F' genotype even at the firm, unripe stage.
32714354	9	34	theme	extracts	1452:1459	arg1	polyuronides					1410:1421	polyuronides	1410:1421	polyuronides	1410:1421	Side chain analyses and molecular weight elution profiles of polyuronides and xyloglucans of cell wall extracts revealed fundamental differences between the genotypes, pointing towards a weakening of the structural integrity of cell walls in the fast softening 'AC-F' genotype even at the firm, unripe stage.
32714354	10	35	from	polysaccharides	1680:1694	arg1	walls					1708:1712	the cell walls	1699:1712	the cell walls of 'AC-F'	1699:1722	As a consequence, the polysaccharides in the cell walls of 'AC-F' may be easier to access and hence more susceptible to enzymatic degradation than in 'AC-S', resulting in faster softening.
32714354	9	36	theme	fundamental	1470:1480	arg1	differences					1482:1492	fundamental differences	1470:1492	fundamental differences between the genotypes	1470:1514	Side chain analyses and molecular weight elution profiles of polyuronides and xyloglucans of cell wall extracts revealed fundamental differences between the genotypes, pointing towards a weakening of the structural integrity of cell walls in the fast softening 'AC-F' genotype even at the firm, unripe stage.
32714354	9	37	from	integrity	1564:1572	arg1	genotype					1617:1624	the fast softening 'AC-F' genotype	1591:1624	the fast softening 'AC-F' genotype	1591:1624	Side chain analyses and molecular weight elution profiles of polyuronides and xyloglucans of cell wall extracts revealed fundamental differences between the genotypes, pointing towards a weakening of the structural integrity of cell walls in the fast softening 'AC-F' genotype even at the firm, unripe stage.
32714354	9	38	from	genotype	1617:1624	arg1	weakening					1536:1544	a weakening	1534:1544	a weakening of the structural integrity of cell walls in the fast softening 'AC-F' genotype	1534:1624	Side chain analyses and molecular weight elution profiles of polyuronides and xyloglucans of cell wall extracts revealed fundamental differences between the genotypes, pointing towards a weakening of the structural integrity of cell walls in the fast softening 'AC-F' genotype even at the firm, unripe stage.
32714354	8	39	theme	cell	1301:1304	arg1	wall					1306:1309	the cell wall	1297:1309	the cell wall	1297:1309	In the pectin domain, differences in pectin solubilization and location of methyl-esterified homogalacturonan in the cell wall between 'AC-S' and 'AC-F' were shown.
32714354	9	40	dep	firm	1638:1641	arg1	unripe					1644:1649	unripe	1644:1649	unripe	1644:1649	Side chain analyses and molecular weight elution profiles of polyuronides and xyloglucans of cell wall extracts revealed fundamental differences between the genotypes, pointing towards a weakening of the structural integrity of cell walls in the fast softening 'AC-F' genotype even at the firm, unripe stage.
32714354	5	41	theme	slow	713:716	arg1	genotype					735:742	a slow 'AC-S' softening genotype	711:742	a slow 'AC-S' softening genotype	711:742	chinensis (kiwifruit) genotypes (a fast 'AC-F' and a slow 'AC-S' softening genotype) was examined using a range of compositional, biochemical, structural, and molecular techniques.
32714354	5	41	theme	slow	713:716	arg1	genotypes					682:690	chinensis (kiwifruit) genotypes	660:690	chinensis (kiwifruit) genotypes (a fast 'AC-F' and a slow 'AC-S' softening genotype)	660:743	chinensis (kiwifruit) genotypes (a fast 'AC-F' and a slow 'AC-S' softening genotype) was examined using a range of compositional, biochemical, structural, and molecular techniques.
32714354	0	42	from	Genotypes	25:33	arg1	Fruit					0:4	Fruit	0:4	Fruit From Two Kiwifruit Genotypes With Contrasting Softening Rates	0:66	Fruit From Two Kiwifruit Genotypes With Contrasting Softening Rates Show Differences in the Xyloglucan and Pectin Domains of the Cell Wall.
32714354	8	43	from	location	1247:1254	arg1	solubilization					1228:1241	pectin solubilization	1221:1241	pectin solubilization	1221:1241	In the pectin domain, differences in pectin solubilization and location of methyl-esterified homogalacturonan in the cell wall between 'AC-S' and 'AC-F' were shown.
32714354	8	43	from	location	1247:1254	arg1	wall					1306:1309	the cell wall	1297:1309	the cell wall	1297:1309	In the pectin domain, differences in pectin solubilization and location of methyl-esterified homogalacturonan in the cell wall between 'AC-S' and 'AC-F' were shown.
32714354	9	44	from	weakening	1536:1544	arg1	genotype					1617:1624	the fast softening 'AC-F' genotype	1591:1624	the fast softening 'AC-F' genotype	1591:1624	Side chain analyses and molecular weight elution profiles of polyuronides and xyloglucans of cell wall extracts revealed fundamental differences between the genotypes, pointing towards a weakening of the structural integrity of cell walls in the fast softening 'AC-F' genotype even at the firm, unripe stage.
32714354	2	45	theme	softening	421:429	arg1	patterns					431:438	quite different softening patterns	405:438	quite different softening patterns	405:438	The regulation of this process is complex, with different genotypes demonstrating quite different softening patterns, even when they are closely related.
32714354	7	46	theme	unripe	1058:1063	arg1	stage					1065:1069	the firm unripe stage	1049:1069	the firm unripe stage	1049:1069	In the hemicellulose domain, xyloglucanase enzyme activity was higher in 'AC-F' at the firm unripe stage, a finding supported by differential expression of xyloglucan transglycosylase/hydrolase genes during softening.
32714354	7	46	theme	unripe	1058:1063	arg1	finding					1074:1080	a finding	1072:1080	a finding supported by differential expression of xyloglucan transglycosylase/hydrolase genes during softening	1072:1181	In the hemicellulose domain, xyloglucanase enzyme activity was higher in 'AC-F' at the firm unripe stage, a finding supported by differential expression of xyloglucan transglycosylase/hydrolase genes during softening.
32714354	1	47	theme	sugar	290:294	arg1	matrices					296:303	the complex sugar matrices	278:303	the complex sugar matrices in the cell wall	278:320	Fruit softening is controlled by hormonal and developmental cues, causing an upregulation of cell wall-associated enzymes that break down the complex sugar matrices in the cell wall.
32714354	0	48	theme	Contrasting	40:50	arg1	Rates					62:66	Contrasting Softening Rates	40:66	Contrasting Softening Rates	40:66	Fruit From Two Kiwifruit Genotypes With Contrasting Softening Rates Show Differences in the Xyloglucan and Pectin Domains of the Cell Wall.
32714354	11	49	theme	enzyme	1968:1973	arg1	activities					1975:1984	enzyme activities	1968:1984	enzyme activities alone	1968:1990	Together these results suggest that the different rates of softening between 'AC-F' and 'AC-S' are not due to changes in enzyme activities alone, but that fundamental differences in the cell wall structure are likely to influence the rates of softening through differential modification and accessibility of specific cell wall polysaccharides during ripening.
32714354	11	50	theme	specific	2155:2162	arg1	polysaccharides					2174:2188	specific cell wall polysaccharides	2155:2188	specific cell wall polysaccharides	2155:2188	Together these results suggest that the different rates of softening between 'AC-F' and 'AC-S' are not due to changes in enzyme activities alone, but that fundamental differences in the cell wall structure are likely to influence the rates of softening through differential modification and accessibility of specific cell wall polysaccharides during ripening.
32714354	9	51	theme	structural	1553:1562	arg1	integrity					1564:1572	the structural integrity	1549:1572	the structural integrity of cell walls in the fast softening 'AC-F' genotype	1549:1624	Side chain analyses and molecular weight elution profiles of polyuronides and xyloglucans of cell wall extracts revealed fundamental differences between the genotypes, pointing towards a weakening of the structural integrity of cell walls in the fast softening 'AC-F' genotype even at the firm, unripe stage.
32714354	5	52	theme	structural	803:812	arg1	techniques					829:838	compositional, biochemical, structural, and molecular techniques	775:838	compositional, biochemical, structural, and molecular techniques	775:838	chinensis (kiwifruit) genotypes (a fast 'AC-F' and a slow 'AC-S' softening genotype) was examined using a range of compositional, biochemical, structural, and molecular techniques.
32714354	2	53	theme	process	346:352	arg1	complex					357:363	complex	357:363	complex	357:363	The regulation of this process is complex, with different genotypes demonstrating quite different softening patterns, even when they are closely related.
32714354	2	53	theme	process	346:352	arg1	regulation					327:336	The regulation	323:336	The regulation of this process	323:352	The regulation of this process is complex, with different genotypes demonstrating quite different softening patterns, even when they are closely related.
32714354	5	54	theme	compositional	775:787	arg1	techniques					829:838	compositional, biochemical, structural, and molecular techniques	775:838	compositional, biochemical, structural, and molecular techniques	775:838	chinensis (kiwifruit) genotypes (a fast 'AC-F' and a slow 'AC-S' softening genotype) was examined using a range of compositional, biochemical, structural, and molecular techniques.
32714354	0	55	theme	Softening	52:60	arg1	Rates					62:66	Contrasting Softening Rates	40:66	Contrasting Softening Rates	40:66	Fruit From Two Kiwifruit Genotypes With Contrasting Softening Rates Show Differences in the Xyloglucan and Pectin Domains of the Cell Wall.
32714354	2	56	with	complex	357:363	arg1	genotypes					381:389	different genotypes	371:389	different genotypes demonstrating quite different softening patterns	371:438	The regulation of this process is complex, with different genotypes demonstrating quite different softening patterns, even when they are closely related.
32714354	10	57	theme	enzymatic	1778:1786	arg1	degradation					1788:1798	enzymatic degradation	1778:1798	enzymatic degradation	1778:1798	As a consequence, the polysaccharides in the cell walls of 'AC-F' may be easier to access and hence more susceptible to enzymatic degradation than in 'AC-S', resulting in faster softening.
32714354	0	58	with	Genotypes	25:33	arg1	Rates					62:66	Contrasting Softening Rates	40:66	Contrasting Softening Rates	40:66	Fruit From Two Kiwifruit Genotypes With Contrasting Softening Rates Show Differences in the Xyloglucan and Pectin Domains of the Cell Wall.
32714354	1	59	theme	cell	233:236	arg1	enzymes					254:260	cell wall-associated enzymes	233:260	cell wall-associated enzymes	233:260	Fruit softening is controlled by hormonal and developmental cues, causing an upregulation of cell wall-associated enzymes that break down the complex sugar matrices in the cell wall.
32714354	10	60	from	easier	1731:1736	arg1	AC-S					1809:1812	AC-S	1809:1812	AC-S	1809:1812	As a consequence, the polysaccharides in the cell walls of 'AC-F' may be easier to access and hence more susceptible to enzymatic degradation than in 'AC-S', resulting in faster softening.
32714354	9	61	theme	walls	1582:1586	arg1	integrity					1564:1572	the structural integrity	1549:1572	the structural integrity of cell walls in the fast softening 'AC-F' genotype	1549:1624	Side chain analyses and molecular weight elution profiles of polyuronides and xyloglucans of cell wall extracts revealed fundamental differences between the genotypes, pointing towards a weakening of the structural integrity of cell walls in the fast softening 'AC-F' genotype even at the firm, unripe stage.
32714354	11	62	theme	wall	2169:2172	arg1	polysaccharides					2174:2188	specific cell wall polysaccharides	2155:2188	specific cell wall polysaccharides	2155:2188	Together these results suggest that the different rates of softening between 'AC-F' and 'AC-S' are not due to changes in enzyme activities alone, but that fundamental differences in the cell wall structure are likely to influence the rates of softening through differential modification and accessibility of specific cell wall polysaccharides during ripening.
32714354	1	63	theme	enzymes	254:260	arg1	upregulation					217:228	an upregulation	214:228	an upregulation of cell wall-associated enzymes that break down the complex sugar matrices in the cell wall	214:320	Fruit softening is controlled by hormonal and developmental cues, causing an upregulation of cell wall-associated enzymes that break down the complex sugar matrices in the cell wall.
32714354	9	64	theme	wall	1447:1450	arg1	extracts					1452:1459	cell wall extracts	1442:1459	cell wall extracts	1442:1459	Side chain analyses and molecular weight elution profiles of polyuronides and xyloglucans of cell wall extracts revealed fundamental differences between the genotypes, pointing towards a weakening of the structural integrity of cell walls in the fast softening 'AC-F' genotype even at the firm, unripe stage.
32714354	9	65	theme	Side	1349:1352	arg1	chain					1354:1358	Side chain	1349:1358	Side chain analyses	1349:1367	Side chain analyses and molecular weight elution profiles of polyuronides and xyloglucans of cell wall extracts revealed fundamental differences between the genotypes, pointing towards a weakening of the structural integrity of cell walls in the fast softening 'AC-F' genotype even at the firm, unripe stage.
32714354	9	66	theme	softening	1600:1608	arg1	genotype					1617:1624	the fast softening 'AC-F' genotype	1591:1624	the fast softening 'AC-F' genotype	1591:1624	Side chain analyses and molecular weight elution profiles of polyuronides and xyloglucans of cell wall extracts revealed fundamental differences between the genotypes, pointing towards a weakening of the structural integrity of cell walls in the fast softening 'AC-F' genotype even at the firm, unripe stage.
32714354	7	67	theme	enzyme	1009:1014	arg1	activity					1016:1023	xyloglucanase enzyme activity	995:1023	xyloglucanase enzyme activity	995:1023	In the hemicellulose domain, xyloglucanase enzyme activity was higher in 'AC-F' at the firm unripe stage, a finding supported by differential expression of xyloglucan transglycosylase/hydrolase genes during softening.
32714354	6	68	from	stages	958:963	arg1	structure					877:885	the cell wall structure	863:885	the cell wall structure of the two genotypes	863:906	Throughout softening, the cell wall structure of the two genotypes was fundamentally different at identical firmness stages.
32714354	6	68	from	stages	958:963	arg1	different					926:934	different	926:934	different	926:934	Throughout softening, the cell wall structure of the two genotypes was fundamentally different at identical firmness stages.
32714354	9	69	theme	AC-F	1611:1614	arg1	genotype					1617:1624	the fast softening 'AC-F' genotype	1591:1624	the fast softening 'AC-F' genotype	1591:1624	Side chain analyses and molecular weight elution profiles of polyuronides and xyloglucans of cell wall extracts revealed fundamental differences between the genotypes, pointing towards a weakening of the structural integrity of cell walls in the fast softening 'AC-F' genotype even at the firm, unripe stage.
32714354	11	70	from	changes	1957:1963	arg1	activities					1975:1984	enzyme activities	1968:1984	enzyme activities alone	1968:1990	Together these results suggest that the different rates of softening between 'AC-F' and 'AC-S' are not due to changes in enzyme activities alone, but that fundamental differences in the cell wall structure are likely to influence the rates of softening through differential modification and accessibility of specific cell wall polysaccharides during ripening.
32714354	3	71	theme	cell	535:538	arg1	structure					545:553	cell wall structure	535:553	cell wall structure	535:553	Currently, little is known about the relationship between cell wall structure and the rate of fruit softening.
32714354	9	72	theme	weight	1383:1388	arg1	profiles					1398:1405	molecular weight elution profiles	1373:1405	molecular weight elution profiles	1373:1405	Side chain analyses and molecular weight elution profiles of polyuronides and xyloglucans of cell wall extracts revealed fundamental differences between the genotypes, pointing towards a weakening of the structural integrity of cell walls in the fast softening 'AC-F' genotype even at the firm, unripe stage.
32714354	7	73	theme	hemicellulose	973:985	arg1	domain					987:992	the hemicellulose domain	969:992	the hemicellulose domain	969:992	In the hemicellulose domain, xyloglucanase enzyme activity was higher in 'AC-F' at the firm unripe stage, a finding supported by differential expression of xyloglucan transglycosylase/hydrolase genes during softening.
32714354	5	74	theme	biochemical	790:800	arg1	techniques					829:838	compositional, biochemical, structural, and molecular techniques	775:838	compositional, biochemical, structural, and molecular techniques	775:838	chinensis (kiwifruit) genotypes (a fast 'AC-F' and a slow 'AC-S' softening genotype) was examined using a range of compositional, biochemical, structural, and molecular techniques.
32714354	9	75	theme	elution	1390:1396	arg1	profiles					1398:1405	molecular weight elution profiles	1373:1405	molecular weight elution profiles	1373:1405	Side chain analyses and molecular weight elution profiles of polyuronides and xyloglucans of cell wall extracts revealed fundamental differences between the genotypes, pointing towards a weakening of the structural integrity of cell walls in the fast softening 'AC-F' genotype even at the firm, unripe stage.
32714354	6	76	theme	cell	867:870	arg1	structure					877:885	the cell wall structure	863:885	the cell wall structure of the two genotypes	863:906	Throughout softening, the cell wall structure of the two genotypes was fundamentally different at identical firmness stages.
32714354	6	76	theme	cell	867:870	arg1	different					926:934	different	926:934	different	926:934	Throughout softening, the cell wall structure of the two genotypes was fundamentally different at identical firmness stages.
32714354	7	77	theme	differential	1095:1106	arg1	expression					1108:1117	differential expression	1095:1117	differential expression of xyloglucan transglycosylase/hydrolase genes during softening	1095:1181	In the hemicellulose domain, xyloglucanase enzyme activity was higher in 'AC-F' at the firm unripe stage, a finding supported by differential expression of xyloglucan transglycosylase/hydrolase genes during softening.
32714354	5	78	theme	molecular	819:827	arg1	techniques					829:838	compositional, biochemical, structural, and molecular techniques	775:838	compositional, biochemical, structural, and molecular techniques	775:838	chinensis (kiwifruit) genotypes (a fast 'AC-F' and a slow 'AC-S' softening genotype) was examined using a range of compositional, biochemical, structural, and molecular techniques.
32714354	9	79	theme	firm	1638:1641	arg1	stage					1651:1655	the firm, unripe stage	1634:1655	the firm, unripe stage	1634:1655	Side chain analyses and molecular weight elution profiles of polyuronides and xyloglucans of cell wall extracts revealed fundamental differences between the genotypes, pointing towards a weakening of the structural integrity of cell walls in the fast softening 'AC-F' genotype even at the firm, unripe stage.
32714354	10	80	theme	faster	1829:1834	arg1	softening					1836:1844	faster softening	1829:1844	faster softening	1829:1844	As a consequence, the polysaccharides in the cell walls of 'AC-F' may be easier to access and hence more susceptible to enzymatic degradation than in 'AC-S', resulting in faster softening.
32714354	6	81	theme	firmness	949:956	arg1	stages					958:963	identical firmness stages	939:963	identical firmness stages	939:963	Throughout softening, the cell wall structure of the two genotypes was fundamentally different at identical firmness stages.
32714354	8	82	theme	pectin	1221:1226	arg1	solubilization					1228:1241	pectin solubilization	1221:1241	pectin solubilization	1221:1241	In the pectin domain, differences in pectin solubilization and location of methyl-esterified homogalacturonan in the cell wall between 'AC-S' and 'AC-F' were shown.
32714354	7	83	theme	transglycosylase/hydrolase	1133:1158	arg1	genes					1160:1164	xyloglucan transglycosylase/hydrolase genes	1122:1164	xyloglucan transglycosylase/hydrolase genes	1122:1164	In the hemicellulose domain, xyloglucanase enzyme activity was higher in 'AC-F' at the firm unripe stage, a finding supported by differential expression of xyloglucan transglycosylase/hydrolase genes during softening.
32714354	3	84	theme	softening	577:585	arg1	relationship					514:525	the relationship	510:525	the relationship between cell wall structure	510:553	Currently, little is known about the relationship between cell wall structure and the rate of fruit softening.
32714354	3	84	theme	softening	577:585	arg1	rate					563:566	the rate	559:566	the rate of fruit softening	559:585	Currently, little is known about the relationship between cell wall structure and the rate of fruit softening.
32714354	9	85	theme	molecular	1373:1381	arg1	profiles					1398:1405	molecular weight elution profiles	1373:1405	molecular weight elution profiles	1373:1405	Side chain analyses and molecular weight elution profiles of polyuronides and xyloglucans of cell wall extracts revealed fundamental differences between the genotypes, pointing towards a weakening of the structural integrity of cell walls in the fast softening 'AC-F' genotype even at the firm, unripe stage.
32714354	5	86	dep	genotypes	682:690	arg1	genotype					735:742	a slow 'AC-S' softening genotype	711:742	a slow 'AC-S' softening genotype	711:742	chinensis (kiwifruit) genotypes (a fast 'AC-F' and a slow 'AC-S' softening genotype) was examined using a range of compositional, biochemical, structural, and molecular techniques.
32714354	5	86	dep	genotypes	682:690	arg1	AC-F					701:704	a fast 'AC-F'	693:705	a fast 'AC-F'	693:705	chinensis (kiwifruit) genotypes (a fast 'AC-F' and a slow 'AC-S' softening genotype) was examined using a range of compositional, biochemical, structural, and molecular techniques.
32714354	5	86	dep	genotypes	682:690	arg1	genotypes					682:690	chinensis (kiwifruit) genotypes	660:690	chinensis (kiwifruit) genotypes (a fast 'AC-F' and a slow 'AC-S' softening genotype)	660:743	chinensis (kiwifruit) genotypes (a fast 'AC-F' and a slow 'AC-S' softening genotype) was examined using a range of compositional, biochemical, structural, and molecular techniques.
32714354	4	87	theme	chinensis	645:653	arg1	var					655:657	two Actinidia chinensis var	631:657	two Actinidia chinensis var	631:657	To address this question, the softening of two Actinidia chinensis var.
32714354	1	88	theme	Fruit	140:144	arg1	softening					146:154	Fruit softening	140:154	Fruit softening	140:154	Fruit softening is controlled by hormonal and developmental cues, causing an upregulation of cell wall-associated enzymes that break down the complex sugar matrices in the cell wall.
32714354	11	89	theme	cell	2033:2036	arg1	structure					2043:2051	the cell wall structure	2029:2051	the cell wall structure	2029:2051	Together these results suggest that the different rates of softening between 'AC-F' and 'AC-S' are not due to changes in enzyme activities alone, but that fundamental differences in the cell wall structure are likely to influence the rates of softening through differential modification and accessibility of specific cell wall polysaccharides during ripening.
32714354	0	90	theme	Xyloglucan	92:101	arg1	Domains					114:120	the Xyloglucan and Pectin Domains	88:120	Domains	114:120	Fruit From Two Kiwifruit Genotypes With Contrasting Softening Rates Show Differences in the Xyloglucan and Pectin Domains of the Cell Wall.
32714354	0	90	theme	Xyloglucan	92:101	arg1	Wall					134:137	the Cell Wall	125:137	the Cell Wall	125:137	Fruit From Two Kiwifruit Genotypes With Contrasting Softening Rates Show Differences in the Xyloglucan and Pectin Domains of the Cell Wall.
32714354	11	91	theme	different	1887:1895	arg1	due					1950:1952	due	1950:1952	due	1950:1952	Together these results suggest that the different rates of softening between 'AC-F' and 'AC-S' are not due to changes in enzyme activities alone, but that fundamental differences in the cell wall structure are likely to influence the rates of softening through differential modification and accessibility of specific cell wall polysaccharides during ripening.
32714354	11	91	theme	different	1887:1895	arg1	rates					1897:1901	the different rates	1883:1901	the different rates of softening between 'AC-F' and 'AC-S'	1883:1940	Together these results suggest that the different rates of softening between 'AC-F' and 'AC-S' are not due to changes in enzyme activities alone, but that fundamental differences in the cell wall structure are likely to influence the rates of softening through differential modification and accessibility of specific cell wall polysaccharides during ripening.
32714354	6	92	theme	genotypes	898:906	arg1	structure					877:885	the cell wall structure	863:885	the cell wall structure of the two genotypes	863:906	Throughout softening, the cell wall structure of the two genotypes was fundamentally different at identical firmness stages.
32714354	6	92	theme	genotypes	898:906	arg1	different					926:934	different	926:934	different	926:934	Throughout softening, the cell wall structure of the two genotypes was fundamentally different at identical firmness stages.
32714354	8	93	theme	homogalacturonan	1277:1292	arg1	location					1247:1254	location	1247:1254	location of methyl-esterified homogalacturonan	1247:1292	In the pectin domain, differences in pectin solubilization and location of methyl-esterified homogalacturonan in the cell wall between 'AC-S' and 'AC-F' were shown.
32714354	8	93	theme	homogalacturonan	1277:1292	arg1	differences					1206:1216	differences	1206:1216	differences in pectin solubilization	1206:1241	In the pectin domain, differences in pectin solubilization and location of methyl-esterified homogalacturonan in the cell wall between 'AC-S' and 'AC-F' were shown.
32714354	0	94	theme	Pectin	107:112	arg1	Domains					114:120	the Xyloglucan and Pectin Domains	88:120	Domains	114:120	Fruit From Two Kiwifruit Genotypes With Contrasting Softening Rates Show Differences in the Xyloglucan and Pectin Domains of the Cell Wall.
32714354	0	94	theme	Pectin	107:112	arg1	Wall					134:137	the Cell Wall	125:137	the Cell Wall	125:137	Fruit From Two Kiwifruit Genotypes With Contrasting Softening Rates Show Differences in the Xyloglucan and Pectin Domains of the Cell Wall.
32714354	8	95	from	differences	1206:1216	arg1	solubilization					1228:1241	pectin solubilization	1221:1241	pectin solubilization	1221:1241	In the pectin domain, differences in pectin solubilization and location of methyl-esterified homogalacturonan in the cell wall between 'AC-S' and 'AC-F' were shown.
32714354	8	95	from	differences	1206:1216	arg1	wall					1306:1309	the cell wall	1297:1309	the cell wall	1297:1309	In the pectin domain, differences in pectin solubilization and location of methyl-esterified homogalacturonan in the cell wall between 'AC-S' and 'AC-F' were shown.
32714354	9	96	theme	polyuronides	1410:1421	arg1	analyses					1360:1367	Side chain analyses	1349:1367	Side chain analyses	1349:1367	Side chain analyses and molecular weight elution profiles of polyuronides and xyloglucans of cell wall extracts revealed fundamental differences between the genotypes, pointing towards a weakening of the structural integrity of cell walls in the fast softening 'AC-F' genotype even at the firm, unripe stage.
32714354	9	96	theme	polyuronides	1410:1421	arg1	profiles					1398:1405	molecular weight elution profiles	1373:1405	molecular weight elution profiles	1373:1405	Side chain analyses and molecular weight elution profiles of polyuronides and xyloglucans of cell wall extracts revealed fundamental differences between the genotypes, pointing towards a weakening of the structural integrity of cell walls in the fast softening 'AC-F' genotype even at the firm, unripe stage.
32714354	5	97	theme	fast	695:698	arg1	AC-F					701:704	a fast 'AC-F'	693:705	a fast 'AC-F'	693:705	chinensis (kiwifruit) genotypes (a fast 'AC-F' and a slow 'AC-S' softening genotype) was examined using a range of compositional, biochemical, structural, and molecular techniques.
32714354	5	97	theme	fast	695:698	arg1	genotypes					682:690	chinensis (kiwifruit) genotypes	660:690	chinensis (kiwifruit) genotypes (a fast 'AC-F' and a slow 'AC-S' softening genotype)	660:743	chinensis (kiwifruit) genotypes (a fast 'AC-F' and a slow 'AC-S' softening genotype) was examined using a range of compositional, biochemical, structural, and molecular techniques.
32714354	5	98	theme	softening	725:733	arg1	genotype					735:742	a slow 'AC-S' softening genotype	711:742	a slow 'AC-S' softening genotype	711:742	chinensis (kiwifruit) genotypes (a fast 'AC-F' and a slow 'AC-S' softening genotype) was examined using a range of compositional, biochemical, structural, and molecular techniques.
32714354	5	98	theme	softening	725:733	arg1	genotypes					682:690	chinensis (kiwifruit) genotypes	660:690	chinensis (kiwifruit) genotypes (a fast 'AC-F' and a slow 'AC-S' softening genotype)	660:743	chinensis (kiwifruit) genotypes (a fast 'AC-F' and a slow 'AC-S' softening genotype) was examined using a range of compositional, biochemical, structural, and molecular techniques.
32714354	0	99	theme	Cell	129:132	arg1	Wall					134:137	the Cell Wall	125:137	the Cell Wall	125:137	Fruit From Two Kiwifruit Genotypes With Contrasting Softening Rates Show Differences in the Xyloglucan and Pectin Domains of the Cell Wall.
32714354	0	100	theme	Kiwifruit	15:23	arg1	Genotypes					25:33	Two Kiwifruit Genotypes	11:33	Two Kiwifruit Genotypes With Contrasting Softening Rates	11:66	Fruit From Two Kiwifruit Genotypes With Contrasting Softening Rates Show Differences in the Xyloglucan and Pectin Domains of the Cell Wall.
32714354	2	101	theme	different	411:419	arg1	patterns					431:438	quite different softening patterns	405:438	quite different softening patterns	405:438	The regulation of this process is complex, with different genotypes demonstrating quite different softening patterns, even when they are closely related.
32714354	7	102	theme	firm	1053:1056	arg1	stage					1065:1069	the firm unripe stage	1049:1069	the firm unripe stage	1049:1069	In the hemicellulose domain, xyloglucanase enzyme activity was higher in 'AC-F' at the firm unripe stage, a finding supported by differential expression of xyloglucan transglycosylase/hydrolase genes during softening.
32714354	7	102	theme	firm	1053:1056	arg1	finding					1074:1080	a finding	1072:1080	a finding supported by differential expression of xyloglucan transglycosylase/hydrolase genes during softening	1072:1181	In the hemicellulose domain, xyloglucanase enzyme activity was higher in 'AC-F' at the firm unripe stage, a finding supported by differential expression of xyloglucan transglycosylase/hydrolase genes during softening.
32714354	1	103	theme	complex	282:288	arg1	matrices					296:303	the complex sugar matrices	278:303	the complex sugar matrices in the cell wall	278:320	Fruit softening is controlled by hormonal and developmental cues, causing an upregulation of cell wall-associated enzymes that break down the complex sugar matrices in the cell wall.
32714354	2	104	theme	different	371:379	arg1	genotypes					381:389	different genotypes	371:389	different genotypes demonstrating quite different softening patterns	371:438	The regulation of this process is complex, with different genotypes demonstrating quite different softening patterns, even when they are closely related.
32714354	5	105	theme	AC-S	719:722	arg1	genotype					735:742	a slow 'AC-S' softening genotype	711:742	a slow 'AC-S' softening genotype	711:742	chinensis (kiwifruit) genotypes (a fast 'AC-F' and a slow 'AC-S' softening genotype) was examined using a range of compositional, biochemical, structural, and molecular techniques.
32714354	5	105	theme	AC-S	719:722	arg1	genotypes					682:690	chinensis (kiwifruit) genotypes	660:690	chinensis (kiwifruit) genotypes (a fast 'AC-F' and a slow 'AC-S' softening genotype)	660:743	chinensis (kiwifruit) genotypes (a fast 'AC-F' and a slow 'AC-S' softening genotype) was examined using a range of compositional, biochemical, structural, and molecular techniques.
32714354	11	106	theme	fundamental	2002:2012	arg1	differences					2014:2024	fundamental differences	2002:2024	fundamental differences in the cell wall structure	2002:2051	Together these results suggest that the different rates of softening between 'AC-F' and 'AC-S' are not due to changes in enzyme activities alone, but that fundamental differences in the cell wall structure are likely to influence the rates of softening through differential modification and accessibility of specific cell wall polysaccharides during ripening.
32714354	10	107	theme	cell	1703:1706	arg1	walls					1708:1712	the cell walls	1699:1712	the cell walls of 'AC-F'	1699:1722	As a consequence, the polysaccharides in the cell walls of 'AC-F' may be easier to access and hence more susceptible to enzymatic degradation than in 'AC-S', resulting in faster softening.
32714354	11	108	theme	cell	2164:2167	arg1	polysaccharides					2174:2188	specific cell wall polysaccharides	2155:2188	specific cell wall polysaccharides	2155:2188	Together these results suggest that the different rates of softening between 'AC-F' and 'AC-S' are not due to changes in enzyme activities alone, but that fundamental differences in the cell wall structure are likely to influence the rates of softening through differential modification and accessibility of specific cell wall polysaccharides during ripening.
32714354	9	109	theme	integrity	1564:1572	arg1	weakening					1536:1544	a weakening	1534:1544	a weakening of the structural integrity of cell walls in the fast softening 'AC-F' genotype	1534:1624	Side chain analyses and molecular weight elution profiles of polyuronides and xyloglucans of cell wall extracts revealed fundamental differences between the genotypes, pointing towards a weakening of the structural integrity of cell walls in the fast softening 'AC-F' genotype even at the firm, unripe stage.
34721434	4	0	theme	derived	752:758	arg1	LPS					760:762	coli- derived LPS	746:762	coli- derived LPS (LPS, ANT+LPS and LA+LPS groups)	746:795	After 42 days of feeding, three groups were intraperitoneally injected with 0.5 mg/kg Escherichia coli- derived LPS (LPS, ANT+LPS and LA+LPS groups) for three consecutive days, and the control (CON) group was injected with the same volume of saline.
34721434	7	1	theme	immune	1077:1082	arg1	functions					1084:1092	immune functions	1077:1092	immune functions	1077:1092	LA enhanced immune functions and inhibited inflammatory responses by upregulating the concentrations of immunoglobulins (IgA, IgM, and IgY), decreasing IL-6 and increasing IL-4 and IL-10.
34721434	13	2	from	challenge	2162:2170	arg1	production					2182:2191	animal production	2175:2191	animal production	2175:2191	The results highlight the potential of LA as a dietary supplement to combat bacterial LPS challenge in animal production and to promote food safety.
34721434	2	3	from	effects	285:291	arg1	barrier					317:323	the intestinal barrier	302:323	the intestinal barrier	302:323	This study aimed to investigate the effects of LA on the intestinal barrier, immune functions, serum metabolism, and gut microbiota of broilers under lipopolysaccharide (LPS) challenge.
34721434	6	4	theme	mucosal	1023:1029	arg1	injuries					1031:1038	intestinal mucosal injuries	1012:1038	intestinal mucosal injuries	1012:1038	Results showed that LA pretreatment significantly alleviated the weight loss and intestinal mucosal injuries caused by LPS challenge.
34721434	4	5	theme	saline	890:895	arg1	volume					880:885	the same volume	871:885	the same volume of saline	871:895	After 42 days of feeding, three groups were intraperitoneally injected with 0.5 mg/kg Escherichia coli- derived LPS (LPS, ANT+LPS and LA+LPS groups) for three consecutive days, and the control (CON) group was injected with the same volume of saline.
34721434	12	6	theme	serum	2026:2030	arg1	metabolites					2032:2042	serum metabolites	2026:2042	serum metabolites	2026:2042	Pearson's correlation assays showed that alterations in serum metabolism and gut microbiota were strongly correlated to the immune factors; there were also strong correlations between serum metabolites and microbiota composition.
34721434	1	7	theme	crucial	154:160	arg1	acid					139:142	Lauric acid	132:142	Lauric acid (LA)	132:147	Lauric acid (LA) is a crucial medium-chain fatty acid (MCFA) that has many beneficial effects on humans and animals.
34721434	1	7	theme	crucial	154:160	arg1	MCFA					187:190	MCFA	187:190	MCFA	187:190	Lauric acid (LA) is a crucial medium-chain fatty acid (MCFA) that has many beneficial effects on humans and animals.
34721434	1	7	theme	crucial	154:160	arg1	acid					181:184	a crucial medium-chain fatty acid	152:184	a crucial medium-chain fatty acid (MCFA) that has many beneficial effects on humans and animals	152:246	Lauric acid (LA) is a crucial medium-chain fatty acid (MCFA) that has many beneficial effects on humans and animals.
34721434	12	8	from	alterations	1883:1893	arg1	metabolism					1904:1913	serum metabolism	1898:1913	serum metabolism	1898:1913	Pearson's correlation assays showed that alterations in serum metabolism and gut microbiota were strongly correlated to the immune factors; there were also strong correlations between serum metabolites and microbiota composition.
34721434	12	8	from	alterations	1883:1893	arg1	microbiota					1923:1932	gut microbiota	1919:1932	gut microbiota	1919:1932	Pearson's correlation assays showed that alterations in serum metabolism and gut microbiota were strongly correlated to the immune factors; there were also strong correlations between serum metabolites and microbiota composition.
34721434	6	9	theme	weight	996:1001	arg1	loss					1003:1006	weight loss	996:1006	weight loss	996:1006	Results showed that LA pretreatment significantly alleviated the weight loss and intestinal mucosal injuries caused by LPS challenge.
34721434	3	10	theme	mg/kg	638:642	arg1	LA					644:645	1000 mg/kg LA	633:645	1000 mg/kg LA	633:645	A total of 384 one-day-old broilers were randomly divided into four groups, and fed with a basal diet, or a basal diet supplemented with 75 mg/kg antibiotic (ANT), or a basal diet supplemented with 1000 mg/kg LA.
34721434	1	11	theme	fatty	175:179	arg1	acid					139:142	Lauric acid	132:142	Lauric acid (LA)	132:147	Lauric acid (LA) is a crucial medium-chain fatty acid (MCFA) that has many beneficial effects on humans and animals.
34721434	1	11	theme	fatty	175:179	arg1	MCFA					187:190	MCFA	187:190	MCFA	187:190	Lauric acid (LA) is a crucial medium-chain fatty acid (MCFA) that has many beneficial effects on humans and animals.
34721434	1	11	theme	fatty	175:179	arg1	acid					181:184	a crucial medium-chain fatty acid	152:184	a crucial medium-chain fatty acid (MCFA) that has many beneficial effects on humans and animals	152:246	Lauric acid (LA) is a crucial medium-chain fatty acid (MCFA) that has many beneficial effects on humans and animals.
34721434	13	12	theme	food	2208:2211	arg1	safety					2213:2218	food safety	2208:2218	food safety	2208:2218	The results highlight the potential of LA as a dietary supplement to combat bacterial LPS challenge in animal production and to promote food safety.
34721434	3	13	theme	basal	526:530	arg1	diet					532:535	a basal diet	524:535	a basal diet	524:535	A total of 384 one-day-old broilers were randomly divided into four groups, and fed with a basal diet, or a basal diet supplemented with 75 mg/kg antibiotic (ANT), or a basal diet supplemented with 1000 mg/kg LA.
34721434	11	14	theme	Moreover,16S	1556:1567	arg1	analysis					1574:1581	Moreover,16S rRNA analysis	1556:1581	Moreover,16S rRNA analysis	1556:1581	Moreover,16S rRNA analysis showed that LA inhibited LPS-induced gut dysbiosis by altering cecal microbial composition (reducing Escherichia-Shigella, Barnesiella and Alistipes, and increasing Lactobacillus and Bacteroides), and modulating the production of volatile fatty acids (VFAs).
34721434	8	15	theme	serum	1312:1316	arg1	metabolites					1318:1328	serum metabolites	1312:1328	serum metabolites	1312:1328	Metabolomics analysis revealed a significant difference of serum metabolites by LA pretreatment.
34721434	6	16	theme	LPS	1050:1052	arg1	challenge					1054:1062	LPS challenge	1050:1062	LPS challenge	1050:1062	Results showed that LA pretreatment significantly alleviated the weight loss and intestinal mucosal injuries caused by LPS challenge.
34721434	13	17	theme	bacterial	2148:2156	arg1	challenge					2162:2170	bacterial LPS challenge	2148:2170	bacterial LPS challenge in animal production	2148:2191	The results highlight the potential of LA as a dietary supplement to combat bacterial LPS challenge in animal production and to promote food safety.
34721434	9	18	theme	serum	1363:1367	arg1	biomarkers					1379:1388	Twenty-seven serum metabolic biomarkers	1350:1388	Twenty-seven serum metabolic biomarkers	1350:1388	Twenty-seven serum metabolic biomarkers were identified and mostly belong to lipids.
34721434	11	19	theme	gut	1620:1622	arg1	dysbiosis					1624:1632	LPS-induced gut dysbiosis	1608:1632	LPS-induced gut dysbiosis	1608:1632	Moreover,16S rRNA analysis showed that LA inhibited LPS-induced gut dysbiosis by altering cecal microbial composition (reducing Escherichia-Shigella, Barnesiella and Alistipes, and increasing Lactobacillus and Bacteroides), and modulating the production of volatile fatty acids (VFAs).
34721434	13	20	theme	LPS	2158:2160	arg1	challenge					2162:2170	bacterial LPS challenge	2148:2170	bacterial LPS challenge in animal production	2148:2191	The results highlight the potential of LA as a dietary supplement to combat bacterial LPS challenge in animal production and to promote food safety.
34721434	2	21	from	microbiota	370:379	arg1	barrier					317:323	the intestinal barrier	302:323	the intestinal barrier	302:323	This study aimed to investigate the effects of LA on the intestinal barrier, immune functions, serum metabolism, and gut microbiota of broilers under lipopolysaccharide (LPS) challenge.
34721434	4	22	theme	consecutive	807:817	arg1	days					819:822	three consecutive days	801:822	three consecutive days	801:822	After 42 days of feeding, three groups were intraperitoneally injected with 0.5 mg/kg Escherichia coli- derived LPS (LPS, ANT+LPS and LA+LPS groups) for three consecutive days, and the control (CON) group was injected with the same volume of saline.
34721434	13	23	dep	supplement	2127:2136	arg1	promote					2200:2206	promote	2200:2206	to promote food safety	2197:2218	The results highlight the potential of LA as a dietary supplement to combat bacterial LPS challenge in animal production and to promote food safety.
34721434	13	23	dep	supplement	2127:2136	arg1	combat					2141:2146	combat	2141:2146	to combat bacterial LPS challenge in animal production	2138:2191	The results highlight the potential of LA as a dietary supplement to combat bacterial LPS challenge in animal production and to promote food safety.
34721434	11	24	theme	cecal	1646:1650	arg1	composition					1662:1672	cecal microbial composition	1646:1672	cecal microbial composition (reducing Escherichia-Shigella, Barnesiella and Alistipes, and increasing Lactobacillus and Bacteroides)	1646:1777	Moreover,16S rRNA analysis showed that LA inhibited LPS-induced gut dysbiosis by altering cecal microbial composition (reducing Escherichia-Shigella, Barnesiella and Alistipes, and increasing Lactobacillus and Bacteroides), and modulating the production of volatile fatty acids (VFAs).
34721434	0	25	theme	Acid	83:86	arg1	Benefits					64:71	the Benefits	60:71	the Benefits of Lauric Acid on Lipopolysaccharide- Challenged Broilers	60:129	Integrating Serum Metabolome and Gut Microbiome to Evaluate the Benefits of Lauric Acid on Lipopolysaccharide- Challenged Broilers.
34721434	2	26	theme	serum	344:348	arg1	metabolism					350:359	serum metabolism	344:359	serum metabolism	344:359	This study aimed to investigate the effects of LA on the intestinal barrier, immune functions, serum metabolism, and gut microbiota of broilers under lipopolysaccharide (LPS) challenge.
34721434	13	27	theme	dietary	2119:2125	arg1	supplement					2127:2136	a dietary supplement	2117:2136	a dietary supplement to combat bacterial LPS challenge in animal production and to promote food safety	2117:2218	The results highlight the potential of LA as a dietary supplement to combat bacterial LPS challenge in animal production and to promote food safety.
34721434	13	27	theme	dietary	2119:2125	arg1	potential					2098:2106	the potential	2094:2106	the potential of LA	2094:2112	The results highlight the potential of LA as a dietary supplement to combat bacterial LPS challenge in animal production and to promote food safety.
34721434	4	28	theme	same	875:878	arg1	volume					880:885	the same volume	871:885	the same volume of saline	871:895	After 42 days of feeding, three groups were intraperitoneally injected with 0.5 mg/kg Escherichia coli- derived LPS (LPS, ANT+LPS and LA+LPS groups) for three consecutive days, and the control (CON) group was injected with the same volume of saline.
34721434	2	29	theme	gut	366:368	arg1	microbiota					370:379	gut microbiota	366:379	gut microbiota	366:379	This study aimed to investigate the effects of LA on the intestinal barrier, immune functions, serum metabolism, and gut microbiota of broilers under lipopolysaccharide (LPS) challenge.
34721434	7	30	theme	immunoglobulins	1169:1183	arg1	concentrations					1151:1164	the concentrations	1147:1164	the concentrations of immunoglobulins (IgA, IgM, and IgY)	1147:1203	LA enhanced immune functions and inhibited inflammatory responses by upregulating the concentrations of immunoglobulins (IgA, IgM, and IgY), decreasing IL-6 and increasing IL-4 and IL-10.
34721434	10	31	theme	lipid	1538:1542	arg1	metabolism					1544:1553	lipid metabolism	1538:1553	lipid metabolism	1538:1553	LA also markedly modulated the pathway for sphingolipid metabolism, suggesting its ability to regulate lipid metabolism.
34721434	11	32	theme	fatty	1822:1826	arg1	acids					1828:1832	volatile fatty acids	1813:1832	volatile fatty acids (VFAs)	1813:1839	Moreover,16S rRNA analysis showed that LA inhibited LPS-induced gut dysbiosis by altering cecal microbial composition (reducing Escherichia-Shigella, Barnesiella and Alistipes, and increasing Lactobacillus and Bacteroides), and modulating the production of volatile fatty acids (VFAs).
34721434	11	32	theme	fatty	1822:1826	arg1	VFAs					1835:1838	VFAs	1835:1838	VFAs	1835:1838	Moreover,16S rRNA analysis showed that LA inhibited LPS-induced gut dysbiosis by altering cecal microbial composition (reducing Escherichia-Shigella, Barnesiella and Alistipes, and increasing Lactobacillus and Bacteroides), and modulating the production of volatile fatty acids (VFAs).
34721434	0	33	theme	Gut	33:35	arg1	Microbiome					37:46	Gut Microbiome	33:46	Gut Microbiome	33:46	Integrating Serum Metabolome and Gut Microbiome to Evaluate the Benefits of Lauric Acid on Lipopolysaccharide- Challenged Broilers.
34721434	4	34	dep	LPS	760:762	arg1	ANT+LPS					770:776	ANT+LPS	770:776	ANT+LPS	770:776	After 42 days of feeding, three groups were intraperitoneally injected with 0.5 mg/kg Escherichia coli- derived LPS (LPS, ANT+LPS and LA+LPS groups) for three consecutive days, and the control (CON) group was injected with the same volume of saline.
34721434	4	34	dep	LPS	760:762	arg1	groups					789:794	LA+LPS groups	782:794	LA+LPS groups	782:794	After 42 days of feeding, three groups were intraperitoneally injected with 0.5 mg/kg Escherichia coli- derived LPS (LPS, ANT+LPS and LA+LPS groups) for three consecutive days, and the control (CON) group was injected with the same volume of saline.
34721434	4	34	dep	LPS	760:762	arg1	LPS					765:767	LPS	765:767	LPS	765:767	After 42 days of feeding, three groups were intraperitoneally injected with 0.5 mg/kg Escherichia coli- derived LPS (LPS, ANT+LPS and LA+LPS groups) for three consecutive days, and the control (CON) group was injected with the same volume of saline.
34721434	4	34	dep	LPS	760:762	arg1	Escherichia					734:744	Escherichia	734:744	Escherichia	734:744	After 42 days of feeding, three groups were intraperitoneally injected with 0.5 mg/kg Escherichia coli- derived LPS (LPS, ANT+LPS and LA+LPS groups) for three consecutive days, and the control (CON) group was injected with the same volume of saline.
34721434	2	35	from	functions	333:341	arg1	barrier					317:323	the intestinal barrier	302:323	the intestinal barrier	302:323	This study aimed to investigate the effects of LA on the intestinal barrier, immune functions, serum metabolism, and gut microbiota of broilers under lipopolysaccharide (LPS) challenge.
34721434	11	36	theme	rRNA	1569:1572	arg1	analysis					1574:1581	Moreover,16S rRNA analysis	1556:1581	Moreover,16S rRNA analysis	1556:1581	Moreover,16S rRNA analysis showed that LA inhibited LPS-induced gut dysbiosis by altering cecal microbial composition (reducing Escherichia-Shigella, Barnesiella and Alistipes, and increasing Lactobacillus and Bacteroides), and modulating the production of volatile fatty acids (VFAs).
34721434	6	37	theme	LA	951:952	arg1	pretreatment					954:965	LA pretreatment	951:965	LA pretreatment	951:965	Results showed that LA pretreatment significantly alleviated the weight loss and intestinal mucosal injuries caused by LPS challenge.
34721434	11	38	theme	LPS-induced	1608:1618	arg1	dysbiosis					1624:1632	LPS-induced gut dysbiosis	1608:1632	LPS-induced gut dysbiosis	1608:1632	Moreover,16S rRNA analysis showed that LA inhibited LPS-induced gut dysbiosis by altering cecal microbial composition (reducing Escherichia-Shigella, Barnesiella and Alistipes, and increasing Lactobacillus and Bacteroides), and modulating the production of volatile fatty acids (VFAs).
34721434	8	39	theme	Metabolomics	1253:1264	arg1	analysis					1266:1273	Metabolomics analysis	1253:1273	Metabolomics analysis	1253:1273	Metabolomics analysis revealed a significant difference of serum metabolites by LA pretreatment.
34721434	3	40	theme	one-day-old	450:460	arg1	broilers					462:469	384 one-day-old broilers	446:469	384 one-day-old broilers	446:469	A total of 384 one-day-old broilers were randomly divided into four groups, and fed with a basal diet, or a basal diet supplemented with 75 mg/kg antibiotic (ANT), or a basal diet supplemented with 1000 mg/kg LA.
34721434	1	41	theme	many	202:205	arg1	effects					218:224	many beneficial effects	202:224	many beneficial effects	202:224	Lauric acid (LA) is a crucial medium-chain fatty acid (MCFA) that has many beneficial effects on humans and animals.
34721434	7	42	dep	immunoglobulins	1169:1183	arg1	IgY					1200:1202	IgY	1200:1202	IgY	1200:1202	LA enhanced immune functions and inhibited inflammatory responses by upregulating the concentrations of immunoglobulins (IgA, IgM, and IgY), decreasing IL-6 and increasing IL-4 and IL-10.
34721434	7	42	dep	immunoglobulins	1169:1183	arg1	IgA					1186:1188	IgA	1186:1188	IgA	1186:1188	LA enhanced immune functions and inhibited inflammatory responses by upregulating the concentrations of immunoglobulins (IgA, IgM, and IgY), decreasing IL-6 and increasing IL-4 and IL-10.
34721434	7	42	dep	immunoglobulins	1169:1183	arg1	immunoglobulins					1169:1183	immunoglobulins	1169:1183	immunoglobulins (IgA, IgM, and IgY)	1169:1203	LA enhanced immune functions and inhibited inflammatory responses by upregulating the concentrations of immunoglobulins (IgA, IgM, and IgY), decreasing IL-6 and increasing IL-4 and IL-10.
34721434	7	42	dep	immunoglobulins	1169:1183	arg1	IgM					1191:1193	IgM	1191:1193	IgM	1191:1193	LA enhanced immune functions and inhibited inflammatory responses by upregulating the concentrations of immunoglobulins (IgA, IgM, and IgY), decreasing IL-6 and increasing IL-4 and IL-10.
34721434	2	43	theme	intestinal	306:315	arg1	barrier					317:323	the intestinal barrier	302:323	the intestinal barrier	302:323	This study aimed to investigate the effects of LA on the intestinal barrier, immune functions, serum metabolism, and gut microbiota of broilers under lipopolysaccharide (LPS) challenge.
34721434	4	44	theme	coli-	746:750	arg1	LPS					760:762	coli- derived LPS	746:762	coli- derived LPS (LPS, ANT+LPS and LA+LPS groups)	746:795	After 42 days of feeding, three groups were intraperitoneally injected with 0.5 mg/kg Escherichia coli- derived LPS (LPS, ANT+LPS and LA+LPS groups) for three consecutive days, and the control (CON) group was injected with the same volume of saline.
34721434	6	45	theme	intestinal	1012:1021	arg1	injuries					1031:1038	intestinal mucosal injuries	1012:1038	intestinal mucosal injuries	1012:1038	Results showed that LA pretreatment significantly alleviated the weight loss and intestinal mucosal injuries caused by LPS challenge.
34721434	12	46	theme	serum	1898:1902	arg1	metabolism					1904:1913	serum metabolism	1898:1913	serum metabolism	1898:1913	Pearson's correlation assays showed that alterations in serum metabolism and gut microbiota were strongly correlated to the immune factors; there were also strong correlations between serum metabolites and microbiota composition.
34721434	3	47	theme	basal	604:608	arg1	diet					610:613	a basal diet	602:613	a basal diet supplemented with 1000 mg/kg LA	602:645	A total of 384 one-day-old broilers were randomly divided into four groups, and fed with a basal diet, or a basal diet supplemented with 75 mg/kg antibiotic (ANT), or a basal diet supplemented with 1000 mg/kg LA.
34721434	1	48	theme	medium-chain	162:173	arg1	acid					139:142	Lauric acid	132:142	Lauric acid (LA)	132:147	Lauric acid (LA) is a crucial medium-chain fatty acid (MCFA) that has many beneficial effects on humans and animals.
34721434	1	48	theme	medium-chain	162:173	arg1	MCFA					187:190	MCFA	187:190	MCFA	187:190	Lauric acid (LA) is a crucial medium-chain fatty acid (MCFA) that has many beneficial effects on humans and animals.
34721434	1	48	theme	medium-chain	162:173	arg1	acid					181:184	a crucial medium-chain fatty acid	152:184	a crucial medium-chain fatty acid (MCFA) that has many beneficial effects on humans and animals	152:246	Lauric acid (LA) is a crucial medium-chain fatty acid (MCFA) that has many beneficial effects on humans and animals.
34721434	12	49	theme	correlation	1852:1862	arg1	assays					1864:1869	Pearson's correlation assays	1842:1869	Pearson's correlation assays	1842:1869	Pearson's correlation assays showed that alterations in serum metabolism and gut microbiota were strongly correlated to the immune factors; there were also strong correlations between serum metabolites and microbiota composition.
34721434	4	50	link	derived	752:758	arg1	LPS					760:762	coli- derived LPS	746:762	coli- derived LPS (LPS, ANT+LPS and LA+LPS groups)	746:795	After 42 days of feeding, three groups were intraperitoneally injected with 0.5 mg/kg Escherichia coli- derived LPS (LPS, ANT+LPS and LA+LPS groups) for three consecutive days, and the control (CON) group was injected with the same volume of saline.
34721434	4	51	theme	LA+LPS	782:787	arg1	groups					789:794	LA+LPS groups	782:794	LA+LPS groups	782:794	After 42 days of feeding, three groups were intraperitoneally injected with 0.5 mg/kg Escherichia coli- derived LPS (LPS, ANT+LPS and LA+LPS groups) for three consecutive days, and the control (CON) group was injected with the same volume of saline.
34721434	8	52	theme	significant	1286:1296	arg1	difference					1298:1307	a significant difference	1284:1307	a significant difference of serum metabolites by LA pretreatment	1284:1347	Metabolomics analysis revealed a significant difference of serum metabolites by LA pretreatment.
34721434	6	53	dep	loss	1003:1006	arg1	the					992:994	the	992:994	the	992:994	Results showed that LA pretreatment significantly alleviated the weight loss and intestinal mucosal injuries caused by LPS challenge.
34721434	3	54	theme	1000	633:636	arg1	mg/kg					638:642	mg/kg	638:642	mg/kg	638:642	A total of 384 one-day-old broilers were randomly divided into four groups, and fed with a basal diet, or a basal diet supplemented with 75 mg/kg antibiotic (ANT), or a basal diet supplemented with 1000 mg/kg LA.
34721434	0	55	from	Benefits	64:71	arg1	Broilers					122:129	Lipopolysaccharide- Challenged Broilers	91:129	Lipopolysaccharide- Challenged Broilers	91:129	Integrating Serum Metabolome and Gut Microbiome to Evaluate the Benefits of Lauric Acid on Lipopolysaccharide- Challenged Broilers.
34721434	12	56	theme	strong	1998:2003	arg1	correlations					2005:2016	strong correlations	1998:2016	strong correlations between serum metabolites and microbiota composition	1998:2069	Pearson's correlation assays showed that alterations in serum metabolism and gut microbiota were strongly correlated to the immune factors; there were also strong correlations between serum metabolites and microbiota composition.
34721434	10	57	theme	sphingolipid	1478:1489	arg1	metabolism					1491:1500	sphingolipid metabolism	1478:1500	sphingolipid metabolism	1478:1500	LA also markedly modulated the pathway for sphingolipid metabolism, suggesting its ability to regulate lipid metabolism.
34721434	1	58	contain	has	198:200	arg1	acid					139:142	Lauric acid	132:142	Lauric acid (LA)	132:147	Lauric acid (LA) is a crucial medium-chain fatty acid (MCFA) that has many beneficial effects on humans and animals.
34721434	1	58	contain	has	198:200	arg1	MCFA					187:190	MCFA	187:190	MCFA	187:190	Lauric acid (LA) is a crucial medium-chain fatty acid (MCFA) that has many beneficial effects on humans and animals.
34721434	1	58	contain	has	198:200	arg2	effects					218:224	many beneficial effects	202:224	many beneficial effects	202:224	Lauric acid (LA) is a crucial medium-chain fatty acid (MCFA) that has many beneficial effects on humans and animals.
34721434	1	58	contain	has	198:200	arg1	acid					181:184	a crucial medium-chain fatty acid	152:184	a crucial medium-chain fatty acid (MCFA) that has many beneficial effects on humans and animals	152:246	Lauric acid (LA) is a crucial medium-chain fatty acid (MCFA) that has many beneficial effects on humans and animals.
34721434	13	59	theme	animal	2175:2180	arg1	production					2182:2191	animal production	2175:2191	animal production	2175:2191	The results highlight the potential of LA as a dietary supplement to combat bacterial LPS challenge in animal production and to promote food safety.
34721434	8	60	theme	metabolites	1318:1328	arg1	difference					1298:1307	a significant difference	1284:1307	a significant difference of serum metabolites by LA pretreatment	1284:1347	Metabolomics analysis revealed a significant difference of serum metabolites by LA pretreatment.
34721434	12	61	theme	immune	1966:1971	arg1	factors					1973:1979	the immune factors	1962:1979	the immune factors	1962:1979	Pearson's correlation assays showed that alterations in serum metabolism and gut microbiota were strongly correlated to the immune factors; there were also strong correlations between serum metabolites and microbiota composition.
34721434	4	62	theme	feeding	665:671	arg1	days					657:660	42 days	654:660	42 days of feeding	654:671	After 42 days of feeding, three groups were intraperitoneally injected with 0.5 mg/kg Escherichia coli- derived LPS (LPS, ANT+LPS and LA+LPS groups) for three consecutive days, and the control (CON) group was injected with the same volume of saline.
34721434	1	63	theme	Lauric	132:137	arg1	LA					145:146	LA	145:146	LA	145:146	Lauric acid (LA) is a crucial medium-chain fatty acid (MCFA) that has many beneficial effects on humans and animals.
34721434	1	63	theme	Lauric	132:137	arg1	acid					139:142	Lauric acid	132:142	Lauric acid (LA)	132:147	Lauric acid (LA) is a crucial medium-chain fatty acid (MCFA) that has many beneficial effects on humans and animals.
34721434	1	63	theme	Lauric	132:137	arg1	acid					181:184	a crucial medium-chain fatty acid	152:184	a crucial medium-chain fatty acid (MCFA) that has many beneficial effects on humans and animals	152:246	Lauric acid (LA) is a crucial medium-chain fatty acid (MCFA) that has many beneficial effects on humans and animals.
34721434	8	64	theme	LA	1333:1334	arg1	pretreatment					1336:1347	LA pretreatment	1333:1347	LA pretreatment	1333:1347	Metabolomics analysis revealed a significant difference of serum metabolites by LA pretreatment.
34721434	9	65	theme	metabolic	1369:1377	arg1	biomarkers					1379:1388	Twenty-seven serum metabolic biomarkers	1350:1388	Twenty-seven serum metabolic biomarkers	1350:1388	Twenty-seven serum metabolic biomarkers were identified and mostly belong to lipids.
34721434	0	66	theme	Lauric	76:81	arg1	Acid					83:86	Lauric Acid	76:86	Lauric Acid	76:86	Integrating Serum Metabolome and Gut Microbiome to Evaluate the Benefits of Lauric Acid on Lipopolysaccharide- Challenged Broilers.
34721434	4	67	theme	control	833:839	arg1	group					847:851	the control (CON) group	829:851	the control (CON) group	829:851	After 42 days of feeding, three groups were intraperitoneally injected with 0.5 mg/kg Escherichia coli- derived LPS (LPS, ANT+LPS and LA+LPS groups) for three consecutive days, and the control (CON) group was injected with the same volume of saline.
34721434	0	68	dep	Challenged	111:120	arg1	Lipopolysaccharide-					91:109	Lipopolysaccharide-	91:109	Lipopolysaccharide-	91:109	Integrating Serum Metabolome and Gut Microbiome to Evaluate the Benefits of Lauric Acid on Lipopolysaccharide- Challenged Broilers.
34721434	11	69	theme	microbial	1652:1660	arg1	composition					1662:1672	cecal microbial composition	1646:1672	cecal microbial composition (reducing Escherichia-Shigella, Barnesiella and Alistipes, and increasing Lactobacillus and Bacteroides)	1646:1777	Moreover,16S rRNA analysis showed that LA inhibited LPS-induced gut dysbiosis by altering cecal microbial composition (reducing Escherichia-Shigella, Barnesiella and Alistipes, and increasing Lactobacillus and Bacteroides), and modulating the production of volatile fatty acids (VFAs).
34721434	0	70	theme	Challenged	111:120	arg1	Broilers					122:129	Lipopolysaccharide- Challenged Broilers	91:129	Lipopolysaccharide- Challenged Broilers	91:129	Integrating Serum Metabolome and Gut Microbiome to Evaluate the Benefits of Lauric Acid on Lipopolysaccharide- Challenged Broilers.
34721434	11	71	theme	volatile	1813:1820	arg1	acids					1828:1832	volatile fatty acids	1813:1832	volatile fatty acids (VFAs)	1813:1839	Moreover,16S rRNA analysis showed that LA inhibited LPS-induced gut dysbiosis by altering cecal microbial composition (reducing Escherichia-Shigella, Barnesiella and Alistipes, and increasing Lactobacillus and Bacteroides), and modulating the production of volatile fatty acids (VFAs).
34721434	11	71	theme	volatile	1813:1820	arg1	VFAs					1835:1838	VFAs	1835:1838	VFAs	1835:1838	Moreover,16S rRNA analysis showed that LA inhibited LPS-induced gut dysbiosis by altering cecal microbial composition (reducing Escherichia-Shigella, Barnesiella and Alistipes, and increasing Lactobacillus and Bacteroides), and modulating the production of volatile fatty acids (VFAs).
34721434	0	72	theme	Serum	12:16	arg1	Metabolome					18:27	Serum Metabolome	12:27	Serum Metabolome	12:27	Integrating Serum Metabolome and Gut Microbiome to Evaluate the Benefits of Lauric Acid on Lipopolysaccharide- Challenged Broilers.
34721434	2	73	theme	broilers	384:391	arg1	effects					285:291	the effects	281:291	the effects of LA on the intestinal barrier	281:323	This study aimed to investigate the effects of LA on the intestinal barrier, immune functions, serum metabolism, and gut microbiota of broilers under lipopolysaccharide (LPS) challenge.
34721434	2	73	theme	broilers	384:391	arg1	microbiota					370:379	gut microbiota	366:379	gut microbiota	366:379	This study aimed to investigate the effects of LA on the intestinal barrier, immune functions, serum metabolism, and gut microbiota of broilers under lipopolysaccharide (LPS) challenge.
34721434	2	73	theme	broilers	384:391	arg1	metabolism					350:359	serum metabolism	344:359	serum metabolism	344:359	This study aimed to investigate the effects of LA on the intestinal barrier, immune functions, serum metabolism, and gut microbiota of broilers under lipopolysaccharide (LPS) challenge.
34721434	2	73	theme	broilers	384:391	arg1	functions					333:341	immune functions	326:341	immune functions	326:341	This study aimed to investigate the effects of LA on the intestinal barrier, immune functions, serum metabolism, and gut microbiota of broilers under lipopolysaccharide (LPS) challenge.
34721434	2	74	theme	immune	326:331	arg1	functions					333:341	immune functions	326:341	immune functions	326:341	This study aimed to investigate the effects of LA on the intestinal barrier, immune functions, serum metabolism, and gut microbiota of broilers under lipopolysaccharide (LPS) challenge.
34721434	4	75	theme	CON	842:844	arg1	group					847:851	the control (CON) group	829:851	the control (CON) group	829:851	After 42 days of feeding, three groups were intraperitoneally injected with 0.5 mg/kg Escherichia coli- derived LPS (LPS, ANT+LPS and LA+LPS groups) for three consecutive days, and the control (CON) group was injected with the same volume of saline.
34721434	3	76	theme	basal	543:547	arg1	diet					549:552	a basal diet	541:552	a basal diet supplemented with 75 mg/kg antibiotic (ANT)	541:596	A total of 384 one-day-old broilers were randomly divided into four groups, and fed with a basal diet, or a basal diet supplemented with 75 mg/kg antibiotic (ANT), or a basal diet supplemented with 1000 mg/kg LA.
34721434	11	77	theme	acids	1828:1832	arg1	production					1799:1808	the production	1795:1808	the production of volatile fatty acids (VFAs)	1795:1839	Moreover,16S rRNA analysis showed that LA inhibited LPS-induced gut dysbiosis by altering cecal microbial composition (reducing Escherichia-Shigella, Barnesiella and Alistipes, and increasing Lactobacillus and Bacteroides), and modulating the production of volatile fatty acids (VFAs).
34721434	13	78	theme	LA	2111:2112	arg1	supplement					2127:2136	a dietary supplement	2117:2136	a dietary supplement to combat bacterial LPS challenge in animal production and to promote food safety	2117:2218	The results highlight the potential of LA as a dietary supplement to combat bacterial LPS challenge in animal production and to promote food safety.
34721434	13	78	theme	LA	2111:2112	arg1	potential					2098:2106	the potential	2094:2106	the potential of LA	2094:2112	The results highlight the potential of LA as a dietary supplement to combat bacterial LPS challenge in animal production and to promote food safety.
34721434	2	79	theme	lipopolysaccharide	399:416	arg1	challenge					424:432	lipopolysaccharide (LPS) challenge	399:432	lipopolysaccharide (LPS) challenge	399:432	This study aimed to investigate the effects of LA on the intestinal barrier, immune functions, serum metabolism, and gut microbiota of broilers under lipopolysaccharide (LPS) challenge.
34721434	3	80	theme	antibiotic	581:590	arg1	mg/kg					575:579	75 mg/kg antibiotic	572:590	75 mg/kg antibiotic (ANT)	572:596	A total of 384 one-day-old broilers were randomly divided into four groups, and fed with a basal diet, or a basal diet supplemented with 75 mg/kg antibiotic (ANT), or a basal diet supplemented with 1000 mg/kg LA.
34721434	3	80	theme	antibiotic	581:590	arg1	ANT					593:595	ANT	593:595	ANT	593:595	A total of 384 one-day-old broilers were randomly divided into four groups, and fed with a basal diet, or a basal diet supplemented with 75 mg/kg antibiotic (ANT), or a basal diet supplemented with 1000 mg/kg LA.
34721434	2	81	theme	LA	296:297	arg1	effects					285:291	the effects	281:291	the effects of LA on the intestinal barrier	281:323	This study aimed to investigate the effects of LA on the intestinal barrier, immune functions, serum metabolism, and gut microbiota of broilers under lipopolysaccharide (LPS) challenge.
34721434	2	81	theme	LA	296:297	arg1	microbiota					370:379	gut microbiota	366:379	gut microbiota	366:379	This study aimed to investigate the effects of LA on the intestinal barrier, immune functions, serum metabolism, and gut microbiota of broilers under lipopolysaccharide (LPS) challenge.
34721434	2	81	theme	LA	296:297	arg1	metabolism					350:359	serum metabolism	344:359	serum metabolism	344:359	This study aimed to investigate the effects of LA on the intestinal barrier, immune functions, serum metabolism, and gut microbiota of broilers under lipopolysaccharide (LPS) challenge.
34721434	2	81	theme	LA	296:297	arg1	functions					333:341	immune functions	326:341	immune functions	326:341	This study aimed to investigate the effects of LA on the intestinal barrier, immune functions, serum metabolism, and gut microbiota of broilers under lipopolysaccharide (LPS) challenge.
34721434	7	82	theme	inflammatory	1108:1119	arg1	responses					1121:1129	inflammatory responses	1108:1129	inflammatory responses	1108:1129	LA enhanced immune functions and inhibited inflammatory responses by upregulating the concentrations of immunoglobulins (IgA, IgM, and IgY), decreasing IL-6 and increasing IL-4 and IL-10.
34721434	12	83	theme	microbiota	2048:2057	arg1	composition					2059:2069	microbiota composition	2048:2069	microbiota composition	2048:2069	Pearson's correlation assays showed that alterations in serum metabolism and gut microbiota were strongly correlated to the immune factors; there were also strong correlations between serum metabolites and microbiota composition.
34721434	12	84	theme	gut	1919:1921	arg1	microbiota					1923:1932	gut microbiota	1919:1932	gut microbiota	1919:1932	Pearson's correlation assays showed that alterations in serum metabolism and gut microbiota were strongly correlated to the immune factors; there were also strong correlations between serum metabolites and microbiota composition.
34721434	2	85	from	metabolism	350:359	arg1	barrier					317:323	the intestinal barrier	302:323	the intestinal barrier	302:323	This study aimed to investigate the effects of LA on the intestinal barrier, immune functions, serum metabolism, and gut microbiota of broilers under lipopolysaccharide (LPS) challenge.
34721434	3	86	theme	broilers	462:469	arg1	total					437:441	A total	435:441	A total of 384 one-day-old broilers	435:469	A total of 384 one-day-old broilers were randomly divided into four groups, and fed with a basal diet, or a basal diet supplemented with 75 mg/kg antibiotic (ANT), or a basal diet supplemented with 1000 mg/kg LA.
34721434	1	87	theme	beneficial	207:216	arg1	effects					218:224	many beneficial effects	202:224	many beneficial effects	202:224	Lauric acid (LA) is a crucial medium-chain fatty acid (MCFA) that has many beneficial effects on humans and animals.
33711371	0	0	theme	intestinal	86:95	arg1	microbiota					97:106	intestinal microbiota	86:106	intestinal microbiota	86:106	Potato resistant starch inhibits diet-induced obesity by modifying the composition of intestinal microbiota and their metabolites in obese mice.
33711371	4	1	theme	Firmicutes/Bacteroidetes	757:780	arg1	rate					788:791	the Firmicutes/Bacteroidetes (F/B) rate	753:791	the Firmicutes/Bacteroidetes (F/B) rate	753:791	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	1	2	theme	starch	162:167	arg1	PRS					177:179	PRS	177:179	PRS	177:179	Potato resistant starch type 3 (PRS) is helpful for weight-loss.
33711371	1	2	theme	starch	162:167	arg1	type					169:172	Potato resistant starch type 3	145:174	Potato resistant starch type 3 (PRS)	145:180	Potato resistant starch type 3 (PRS) is helpful for weight-loss.
33711371	3	3	theme	microbiota	417:426	arg1	composition					428:438	intestinal microbiota composition	406:438	intestinal microbiota composition	406:438	Metabolic syndrome related to obesity, intestinal microbiota composition and its metabolites as well as the relationship among them were studied.
33711371	4	4	theme	metabolites	960:970	arg1	production					936:945	the production	932:945	the production of microbial metabolites, such as propionic acid and acetic acid	932:1010	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	6	5	theme	PRS	1332:1334	arg1	potential					1319:1327	the anti-obesity potential	1302:1327	the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction	1302:1402	It could be concluded that propionic acid and acetic acid were the two dominant metabolites of Bifidobacterium, Ruminococcus, Bacteroides, and Coprococcus, which contributed to the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction.
33711371	4	6	theme	microbiota	840:849	arg1	abundance					816:824	the relative abundance	803:824	the relative abundance of intestinal microbiota	803:849	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	4	7	theme	F/B	783:785	arg1	rate					788:791	the Firmicutes/Bacteroidetes (F/B) rate	753:791	the Firmicutes/Bacteroidetes (F/B) rate	753:791	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	4	8	theme	junction	681:688	arg1	Occludin					708:715	Occludin	708:715	Occludin	708:715	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	4	8	theme	junction	681:688	arg1	occludens					728:736	zonula occludens	721:736	zonula occludens	721:736	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	4	8	theme	junction	681:688	arg1	proteins					690:697	tight junction proteins	675:697	tight junction proteins	675:697	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	4	9	theme	expression	661:670	arg1	proliferation					623:635	the proliferation	619:635	the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus	619:917	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	0	10	theme	microbiota	97:106	arg1	composition					71:81	the composition	67:81	the composition of intestinal microbiota and their metabolites in obese mice	67:142	Potato resistant starch inhibits diet-induced obesity by modifying the composition of intestinal microbiota and their metabolites in obese mice.
33711371	4	11	theme	tight	675:679	arg1	Occludin					708:715	Occludin	708:715	Occludin	708:715	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	4	11	theme	tight	675:679	arg1	occludens					728:736	zonula occludens	721:736	zonula occludens	721:736	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	4	11	theme	tight	675:679	arg1	proteins					690:697	tight junction proteins	675:697	tight junction proteins	675:697	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	6	12	theme	acetic	1171:1176	arg1	metabolites					1205:1215	the two dominant metabolites	1188:1215	the two dominant metabolites	1188:1215	It could be concluded that propionic acid and acetic acid were the two dominant metabolites of Bifidobacterium, Ruminococcus, Bacteroides, and Coprococcus, which contributed to the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction.
33711371	6	12	theme	acetic	1171:1176	arg1	acid					1178:1181	acetic acid	1171:1181	acetic acid	1171:1181	It could be concluded that propionic acid and acetic acid were the two dominant metabolites of Bifidobacterium, Ruminococcus, Bacteroides, and Coprococcus, which contributed to the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction.
33711371	6	12	theme	acetic	1171:1176	arg1	acid					1162:1165	propionic acid	1152:1165	propionic acid	1152:1165	It could be concluded that propionic acid and acetic acid were the two dominant metabolites of Bifidobacterium, Ruminococcus, Bacteroides, and Coprococcus, which contributed to the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction.
33711371	2	13	dep	PRS	314:316	arg1	%					325:325	15%	323:325	15%	323:325	To investigate the regulatory effects of PRS on high-fat diet (HFD)-induced obesity, different doses of PRS (5%, 15% and 25%) were fed to mice for 12 weeks.
33711371	2	13	dep	PRS	314:316	arg1	%					333:333	25%	331:333	25%	331:333	To investigate the regulatory effects of PRS on high-fat diet (HFD)-induced obesity, different doses of PRS (5%, 15% and 25%) were fed to mice for 12 weeks.
33711371	2	13	dep	PRS	314:316	arg1	%					320:320	5%	319:320	5%	319:320	To investigate the regulatory effects of PRS on high-fat diet (HFD)-induced obesity, different doses of PRS (5%, 15% and 25%) were fed to mice for 12 weeks.
33711371	4	14	dep	cells	651:655	arg1	Ruminococcus					877:888	intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus	640:917	Ruminococcus	877:888	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	0	15	theme	metabolites	118:128	arg1	composition					71:81	the composition	67:81	the composition of intestinal microbiota and their metabolites in obese mice	67:142	Potato resistant starch inhibits diet-induced obesity by modifying the composition of intestinal microbiota and their metabolites in obese mice.
33711371	2	16	theme	high-fat	258:265	arg1	diet					267:270	high-fat diet	258:270	high-fat diet (HFD)	258:276	To investigate the regulatory effects of PRS on high-fat diet (HFD)-induced obesity, different doses of PRS (5%, 15% and 25%) were fed to mice for 12 weeks.
33711371	2	16	theme	high-fat	258:265	arg1	HFD					273:275	HFD	273:275	HFD	273:275	To investigate the regulatory effects of PRS on high-fat diet (HFD)-induced obesity, different doses of PRS (5%, 15% and 25%) were fed to mice for 12 weeks.
33711371	6	17	theme	dysfunction	1392:1402	arg1	potential					1319:1327	the anti-obesity potential	1302:1327	the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction	1302:1402	It could be concluded that propionic acid and acetic acid were the two dominant metabolites of Bifidobacterium, Ruminococcus, Bacteroides, and Coprococcus, which contributed to the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction.
33711371	6	18	theme	Bacteroides	1251:1261	arg1	metabolites					1205:1215	the two dominant metabolites	1188:1215	the two dominant metabolites	1188:1215	It could be concluded that propionic acid and acetic acid were the two dominant metabolites of Bifidobacterium, Ruminococcus, Bacteroides, and Coprococcus, which contributed to the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction.
33711371	6	18	theme	Bacteroides	1251:1261	arg1	acid					1162:1165	propionic acid	1152:1165	propionic acid	1152:1165	It could be concluded that propionic acid and acetic acid were the two dominant metabolites of Bifidobacterium, Ruminococcus, Bacteroides, and Coprococcus, which contributed to the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction.
33711371	6	18	theme	Bacteroides	1251:1261	arg1	acid					1178:1181	acetic acid	1171:1181	acetic acid	1171:1181	It could be concluded that propionic acid and acetic acid were the two dominant metabolites of Bifidobacterium, Ruminococcus, Bacteroides, and Coprococcus, which contributed to the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction.
33711371	4	19	theme	proteins	690:697	arg1	expression					661:670	expression	661:670	expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota,	661:850	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	4	19	theme	proteins	690:697	arg1	Bifidobacterium					860:874	Bifidobacterium	860:874	Bifidobacterium	860:874	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	4	19	theme	proteins	690:697	arg1	cells					651:655	intestinal cells	640:655	intestinal cells	640:655	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	4	19	theme	proteins	690:697	arg1	Coprococcus					907:917	Coprococcus	907:917	Coprococcus	907:917	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	4	19	theme	proteins	690:697	arg1	Bacteroides					891:901	Bacteroides	891:901	Bacteroides	891:901	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	4	20	dep	Occludin	708:715	arg1	reduce					746:751	reduce	746:751	reduce the Firmicutes/Bacteroidetes (F/B) rate	746:791	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	4	20	dep	Occludin	708:715	arg1	regulate					794:801	regulate	794:801	regulate the relative abundance of intestinal microbiota	794:849	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	0	21	theme	resistant	7:15	arg1	starch					17:22	Potato resistant starch	0:22	Potato resistant starch	0:22	Potato resistant starch inhibits diet-induced obesity by modifying the composition of intestinal microbiota and their metabolites in obese mice.
33711371	6	22	theme	propionic	1152:1160	arg1	acid					1162:1165	propionic acid	1152:1165	propionic acid	1152:1165	It could be concluded that propionic acid and acetic acid were the two dominant metabolites of Bifidobacterium, Ruminococcus, Bacteroides, and Coprococcus, which contributed to the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction.
33711371	6	22	theme	propionic	1152:1160	arg1	metabolites					1205:1215	the two dominant metabolites	1188:1215	the two dominant metabolites	1188:1215	It could be concluded that propionic acid and acetic acid were the two dominant metabolites of Bifidobacterium, Ruminococcus, Bacteroides, and Coprococcus, which contributed to the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction.
33711371	6	22	theme	propionic	1152:1160	arg1	acid					1178:1181	acetic acid	1171:1181	acetic acid	1171:1181	It could be concluded that propionic acid and acetic acid were the two dominant metabolites of Bifidobacterium, Ruminococcus, Bacteroides, and Coprococcus, which contributed to the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction.
33711371	4	23	theme	dose	588:591	arg1	manner					603:608	a dose dependent manner	586:608	a dose dependent manner	586:608	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	0	24	theme	Potato	0:5	arg1	starch					17:22	Potato resistant starch	0:22	Potato resistant starch	0:22	Potato resistant starch inhibits diet-induced obesity by modifying the composition of intestinal microbiota and their metabolites in obese mice.
33711371	5	25	theme	microbiota	1055:1064	arg1	composition					1066:1076	the intestinal microbiota composition	1040:1076	the intestinal microbiota composition	1040:1076	Besides, the alteration in the intestinal microbiota composition and metabolites were significantly correlated.
33711371	2	26	theme	-induced	277:284	arg1	obesity					286:292	high-fat diet (HFD)-induced obesity	258:292	high-fat diet (HFD)-induced obesity	258:292	To investigate the regulatory effects of PRS on high-fat diet (HFD)-induced obesity, different doses of PRS (5%, 15% and 25%) were fed to mice for 12 weeks.
33711371	2	27	from	effects	240:246	arg1	obesity					286:292	high-fat diet (HFD)-induced obesity	258:292	high-fat diet (HFD)-induced obesity	258:292	To investigate the regulatory effects of PRS on high-fat diet (HFD)-induced obesity, different doses of PRS (5%, 15% and 25%) were fed to mice for 12 weeks.
33711371	0	28	theme	obese	133:137	arg1	mice					139:142	obese mice	133:142	obese mice	133:142	Potato resistant starch inhibits diet-induced obesity by modifying the composition of intestinal microbiota and their metabolites in obese mice.
33711371	3	29	theme	Metabolic	367:375	arg1	syndrome					377:384	Metabolic syndrome	367:384	Metabolic syndrome related to obesity	367:403	Metabolic syndrome related to obesity, intestinal microbiota composition and its metabolites as well as the relationship among them were studied.
33711371	4	30	theme	cells	651:655	arg1	proliferation					623:635	the proliferation	619:635	the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus	619:917	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	6	31	theme	Ruminococcus	1237:1248	arg1	metabolites					1205:1215	the two dominant metabolites	1188:1215	the two dominant metabolites	1188:1215	It could be concluded that propionic acid and acetic acid were the two dominant metabolites of Bifidobacterium, Ruminococcus, Bacteroides, and Coprococcus, which contributed to the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction.
33711371	6	31	theme	Ruminococcus	1237:1248	arg1	acid					1162:1165	propionic acid	1152:1165	propionic acid	1152:1165	It could be concluded that propionic acid and acetic acid were the two dominant metabolites of Bifidobacterium, Ruminococcus, Bacteroides, and Coprococcus, which contributed to the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction.
33711371	6	31	theme	Ruminococcus	1237:1248	arg1	acid					1178:1181	acetic acid	1171:1181	acetic acid	1171:1181	It could be concluded that propionic acid and acetic acid were the two dominant metabolites of Bifidobacterium, Ruminococcus, Bacteroides, and Coprococcus, which contributed to the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction.
33711371	2	32	theme	PRS	314:316	arg1	doses					305:309	different doses	295:309	different doses of PRS (5%, 15% and 25%)	295:334	To investigate the regulatory effects of PRS on high-fat diet (HFD)-induced obesity, different doses of PRS (5%, 15% and 25%) were fed to mice for 12 weeks.
33711371	3	33	theme	intestinal	406:415	arg1	composition					428:438	intestinal microbiota composition	406:438	intestinal microbiota composition	406:438	Metabolic syndrome related to obesity, intestinal microbiota composition and its metabolites as well as the relationship among them were studied.
33711371	6	34	theme	alleviation	1356:1366	arg1	potential					1319:1327	the anti-obesity potential	1302:1327	the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction	1302:1402	It could be concluded that propionic acid and acetic acid were the two dominant metabolites of Bifidobacterium, Ruminococcus, Bacteroides, and Coprococcus, which contributed to the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction.
33711371	6	35	theme	syndrome	1347:1354	arg1	alleviation					1356:1366	metabolic syndrome alleviation	1337:1366	metabolic syndrome alleviation	1337:1366	It could be concluded that propionic acid and acetic acid were the two dominant metabolites of Bifidobacterium, Ruminococcus, Bacteroides, and Coprococcus, which contributed to the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction.
33711371	4	36	theme	acetic	1000:1005	arg1	acid					1007:1010	acetic acid	1000:1010	acetic acid	1000:1010	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	4	37	theme	metabolic	564:572	arg1	syndrome					574:581	HFD-induced metabolic syndrome	552:581	HFD-induced metabolic syndrome	552:581	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	0	38	theme	diet-induced	33:44	arg1	obesity					46:52	diet-induced obesity	33:52	diet-induced obesity	33:52	Potato resistant starch inhibits diet-induced obesity by modifying the composition of intestinal microbiota and their metabolites in obese mice.
33711371	6	39	theme	Bifidobacterium	1220:1234	arg1	metabolites					1205:1215	the two dominant metabolites	1188:1215	the two dominant metabolites	1188:1215	It could be concluded that propionic acid and acetic acid were the two dominant metabolites of Bifidobacterium, Ruminococcus, Bacteroides, and Coprococcus, which contributed to the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction.
33711371	6	39	theme	Bifidobacterium	1220:1234	arg1	acid					1162:1165	propionic acid	1152:1165	propionic acid	1152:1165	It could be concluded that propionic acid and acetic acid were the two dominant metabolites of Bifidobacterium, Ruminococcus, Bacteroides, and Coprococcus, which contributed to the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction.
33711371	6	39	theme	Bifidobacterium	1220:1234	arg1	acid					1178:1181	acetic acid	1171:1181	acetic acid	1171:1181	It could be concluded that propionic acid and acetic acid were the two dominant metabolites of Bifidobacterium, Ruminococcus, Bacteroides, and Coprococcus, which contributed to the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction.
33711371	0	40	from	composition	71:81	arg1	mice					139:142	obese mice	133:142	obese mice	133:142	Potato resistant starch inhibits diet-induced obesity by modifying the composition of intestinal microbiota and their metabolites in obese mice.
33711371	4	41	theme	zonula	721:726	arg1	occludens					728:736	zonula occludens	721:736	zonula occludens	721:736	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	4	42	theme	HFD-induced	552:562	arg1	syndrome					574:581	HFD-induced metabolic syndrome	552:581	HFD-induced metabolic syndrome	552:581	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	6	43	theme	anti-obesity	1306:1317	arg1	potential					1319:1327	the anti-obesity potential	1302:1327	the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction	1302:1402	It could be concluded that propionic acid and acetic acid were the two dominant metabolites of Bifidobacterium, Ruminococcus, Bacteroides, and Coprococcus, which contributed to the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction.
33711371	6	44	theme	Coprococcus	1268:1278	arg1	metabolites					1205:1215	the two dominant metabolites	1188:1215	the two dominant metabolites	1188:1215	It could be concluded that propionic acid and acetic acid were the two dominant metabolites of Bifidobacterium, Ruminococcus, Bacteroides, and Coprococcus, which contributed to the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction.
33711371	6	44	theme	Coprococcus	1268:1278	arg1	acid					1162:1165	propionic acid	1152:1165	propionic acid	1152:1165	It could be concluded that propionic acid and acetic acid were the two dominant metabolites of Bifidobacterium, Ruminococcus, Bacteroides, and Coprococcus, which contributed to the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction.
33711371	6	44	theme	Coprococcus	1268:1278	arg1	acid					1178:1181	acetic acid	1171:1181	acetic acid	1171:1181	It could be concluded that propionic acid and acetic acid were the two dominant metabolites of Bifidobacterium, Ruminococcus, Bacteroides, and Coprococcus, which contributed to the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction.
33711371	6	45	theme	metabolic	1337:1345	arg1	syndrome					1347:1354	metabolic syndrome	1337:1354	metabolic syndrome alleviation	1337:1366	It could be concluded that propionic acid and acetic acid were the two dominant metabolites of Bifidobacterium, Ruminococcus, Bacteroides, and Coprococcus, which contributed to the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction.
33711371	4	46	theme	relative	807:814	arg1	abundance					816:824	the relative abundance	803:824	the relative abundance of intestinal microbiota	803:849	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	4	47	theme	intestinal	640:649	arg1	Bifidobacterium					860:874	Bifidobacterium	860:874	Bifidobacterium	860:874	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	4	47	theme	intestinal	640:649	arg1	cells					651:655	intestinal cells	640:655	intestinal cells	640:655	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	4	47	theme	intestinal	640:649	arg1	Coprococcus					907:917	Coprococcus	907:917	Coprococcus	907:917	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	4	47	theme	intestinal	640:649	arg1	Bacteroides					891:901	Bacteroides	891:901	Bacteroides	891:901	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	5	48	theme	intestinal	1044:1053	arg1	composition					1066:1076	the intestinal microbiota composition	1040:1076	the intestinal microbiota composition	1040:1076	Besides, the alteration in the intestinal microbiota composition and metabolites were significantly correlated.
33711371	6	49	theme	dominant	1196:1203	arg1	metabolites					1205:1215	the two dominant metabolites	1188:1215	the two dominant metabolites	1188:1215	It could be concluded that propionic acid and acetic acid were the two dominant metabolites of Bifidobacterium, Ruminococcus, Bacteroides, and Coprococcus, which contributed to the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction.
33711371	6	49	theme	dominant	1196:1203	arg1	acid					1162:1165	propionic acid	1152:1165	propionic acid	1152:1165	It could be concluded that propionic acid and acetic acid were the two dominant metabolites of Bifidobacterium, Ruminococcus, Bacteroides, and Coprococcus, which contributed to the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction.
33711371	6	49	theme	dominant	1196:1203	arg1	acid					1178:1181	acetic acid	1171:1181	acetic acid	1171:1181	It could be concluded that propionic acid and acetic acid were the two dominant metabolites of Bifidobacterium, Ruminococcus, Bacteroides, and Coprococcus, which contributed to the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction.
33711371	2	50	theme	PRS	251:253	arg1	effects					240:246	the regulatory effects	225:246	the regulatory effects of PRS on high-fat diet (HFD)-induced obesity	225:292	To investigate the regulatory effects of PRS on high-fat diet (HFD)-induced obesity, different doses of PRS (5%, 15% and 25%) were fed to mice for 12 weeks.
33711371	2	51	theme	different	295:303	arg1	doses					305:309	different doses	295:309	different doses of PRS (5%, 15% and 25%)	295:334	To investigate the regulatory effects of PRS on high-fat diet (HFD)-induced obesity, different doses of PRS (5%, 15% and 25%) were fed to mice for 12 weeks.
33711371	4	52	theme	dependent	593:601	arg1	manner					603:608	a dose dependent manner	586:608	a dose dependent manner	586:608	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	4	53	theme	propionic	981:989	arg1	acid					991:994	propionic acid	981:994	propionic acid	981:994	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	3	54	theme	related	386:392	arg1	syndrome					377:384	Metabolic syndrome	367:384	Metabolic syndrome related to obesity	367:403	Metabolic syndrome related to obesity, intestinal microbiota composition and its metabolites as well as the relationship among them were studied.
33711371	4	55	theme	microbial	950:958	arg1	acid					991:994	propionic acid	981:994	propionic acid	981:994	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	4	55	theme	microbial	950:958	arg1	metabolites					960:970	microbial metabolites	950:970	microbial metabolites	950:970	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	4	55	theme	microbial	950:958	arg1	acid					1007:1010	acetic acid	1000:1010	acetic acid	1000:1010	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
33711371	6	56	theme	intestinal	1373:1382	arg1	dysfunction					1392:1402	intestinal barrier dysfunction	1373:1402	intestinal barrier dysfunction	1373:1402	It could be concluded that propionic acid and acetic acid were the two dominant metabolites of Bifidobacterium, Ruminococcus, Bacteroides, and Coprococcus, which contributed to the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction.
33711371	1	57	theme	Potato	145:150	arg1	PRS					177:179	PRS	177:179	PRS	177:179	Potato resistant starch type 3 (PRS) is helpful for weight-loss.
33711371	1	57	theme	Potato	145:150	arg1	type					169:172	Potato resistant starch type 3	145:174	Potato resistant starch type 3 (PRS)	145:180	Potato resistant starch type 3 (PRS) is helpful for weight-loss.
33711371	6	58	theme	barrier	1384:1390	arg1	dysfunction					1392:1402	intestinal barrier dysfunction	1373:1402	intestinal barrier dysfunction	1373:1402	It could be concluded that propionic acid and acetic acid were the two dominant metabolites of Bifidobacterium, Ruminococcus, Bacteroides, and Coprococcus, which contributed to the anti-obesity potential of PRS, metabolic syndrome alleviation, and intestinal barrier dysfunction.
33711371	2	59	theme	regulatory	229:238	arg1	effects					240:246	the regulatory effects	225:246	the regulatory effects of PRS on high-fat diet (HFD)-induced obesity	225:292	To investigate the regulatory effects of PRS on high-fat diet (HFD)-induced obesity, different doses of PRS (5%, 15% and 25%) were fed to mice for 12 weeks.
33711371	1	60	theme	resistant	152:160	arg1	PRS					177:179	PRS	177:179	PRS	177:179	Potato resistant starch type 3 (PRS) is helpful for weight-loss.
33711371	1	60	theme	resistant	152:160	arg1	type					169:172	Potato resistant starch type 3	145:174	Potato resistant starch type 3 (PRS)	145:180	Potato resistant starch type 3 (PRS) is helpful for weight-loss.
33711371	5	61	from	alteration	1026:1035	arg1	composition					1066:1076	the intestinal microbiota composition	1040:1076	the intestinal microbiota composition	1040:1076	Besides, the alteration in the intestinal microbiota composition and metabolites were significantly correlated.
33711371	5	61	from	alteration	1026:1035	arg1	metabolites					1082:1092	metabolites	1082:1092	metabolites	1082:1092	Besides, the alteration in the intestinal microbiota composition and metabolites were significantly correlated.
33711371	4	62	theme	intestinal	829:838	arg1	microbiota					840:849	intestinal microbiota	829:849	intestinal microbiota	829:849	Results showed that PRS could regulate HFD-induced metabolic syndrome in a dose dependent manner; promote the proliferation of intestinal cells and expression of tight junction proteins, such as Occludin and zonula occludens (ZO)-1; reduce the Firmicutes/Bacteroidetes (F/B) rate; regulate the relative abundance of intestinal microbiota, such as Bifidobacterium, Ruminococcus, Bacteroides and Coprococcus; and promote the production of microbial metabolites, such as propionic acid and acetic acid.
32298636	1	0	theme	target	395:400	arg1	cell					402:405	a target cell	393:405	a target cell	393:405	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32298636	7	1	with	membranes	1452:1460	arg1	compositions					1489:1500	different cholesterol compositions	1467:1500	different cholesterol compositions	1467:1500	We also find that the single-particle kinetics of influenza lipid mixing to target membranes with different cholesterol compositions is independent of receptor binding, suggesting that cholesterol-mediated spatial clustering of viral receptors within the target membrane does not significantly affect IAV hemifusion.
32298636	7	2	theme	target	1445:1450	arg1	membranes					1452:1460	target membranes	1445:1460	target membranes with different cholesterol compositions	1445:1500	We also find that the single-particle kinetics of influenza lipid mixing to target membranes with different cholesterol compositions is independent of receptor binding, suggesting that cholesterol-mediated spatial clustering of viral receptors within the target membrane does not significantly affect IAV hemifusion.
32298636	4	3	theme	viral	834:838	arg1	receptors					840:848	surrogate viral receptors	824:848	surrogate viral receptors	824:848	Here, we develop a fusion assay that uses synthetic DNA-lipid conjugates as surrogate viral receptors to tether virions to target vesicles.
32298636	7	4	theme	single-particle	1391:1405	arg1	independent					1505:1515	independent	1505:1515	independent	1505:1515	We also find that the single-particle kinetics of influenza lipid mixing to target membranes with different cholesterol compositions is independent of receptor binding, suggesting that cholesterol-mediated spatial clustering of viral receptors within the target membrane does not significantly affect IAV hemifusion.
32298636	7	4	theme	single-particle	1391:1405	arg1	kinetics					1407:1414	the single-particle kinetics	1387:1414	the single-particle kinetics of influenza lipid mixing to target membranes with different cholesterol compositions	1387:1500	We also find that the single-particle kinetics of influenza lipid mixing to target membranes with different cholesterol compositions is independent of receptor binding, suggesting that cholesterol-mediated spatial clustering of viral receptors within the target membrane does not significantly affect IAV hemifusion.
32298636	7	5	theme	mixing	1435:1440	arg1	independent					1505:1515	independent	1505:1515	independent	1505:1515	We also find that the single-particle kinetics of influenza lipid mixing to target membranes with different cholesterol compositions is independent of receptor binding, suggesting that cholesterol-mediated spatial clustering of viral receptors within the target membrane does not significantly affect IAV hemifusion.
32298636	7	5	theme	mixing	1435:1440	arg1	kinetics					1407:1414	the single-particle kinetics	1387:1414	the single-particle kinetics of influenza lipid mixing to target membranes with different cholesterol compositions	1387:1500	We also find that the single-particle kinetics of influenza lipid mixing to target membranes with different cholesterol compositions is independent of receptor binding, suggesting that cholesterol-mediated spatial clustering of viral receptors within the target membrane does not significantly affect IAV hemifusion.
32298636	6	6	theme	target	1216:1221	arg1	membrane					1223:1230	the target membrane	1212:1230	the target membrane	1212:1230	Through this approach, we find that cholesterol in the target membrane enhances the efficiency of single-particle IAV lipid mixing, whereas the rate of lipid mixing is independent of cholesterol composition.
32298636	7	7	theme	influenza	1419:1427	arg1	mixing					1435:1440	influenza lipid mixing	1419:1440	influenza lipid mixing	1419:1440	We also find that the single-particle kinetics of influenza lipid mixing to target membranes with different cholesterol compositions is independent of receptor binding, suggesting that cholesterol-mediated spatial clustering of viral receptors within the target membrane does not significantly affect IAV hemifusion.
32298636	4	8	theme	DNA-lipid	800:808	arg1	conjugates					810:819	synthetic DNA-lipid conjugates	790:819	synthetic DNA-lipid conjugates as surrogate viral receptors	790:848	Here, we develop a fusion assay that uses synthetic DNA-lipid conjugates as surrogate viral receptors to tether virions to target vesicles.
32298636	3	9	theme	IAV	706:708	arg1	fusion					710:715	IAV fusion	706:715	IAV fusion	706:715	These properties affect both IAV binding and fusion, which makes it difficult to isolate the role of cholesterol in IAV fusion from receptor binding effects.
32298636	8	10	theme	membrane	1747:1754	arg1	cholesterol					1756:1766	target membrane cholesterol	1740:1766	target membrane cholesterol	1740:1766	These results are consistent with the hypothesis that target membrane cholesterol increases lipid mixing efficiency by altering host membrane curvature.
32298636	1	11	theme	process	185:191	arg1	formation					315:323	the formation	311:323	the formation of a fusion pore through which the viral genome is transferred into a target cell	311:405	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32298636	1	11	theme	process	185:191	arg1	fusion					250:255	fusion	250:255	fusion between the viral envelope and the host membrane	250:304	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32298636	1	11	theme	process	185:191	arg1	stages					141:146	many stages	136:146	many stages	136:146	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32298636	1	11	theme	process	185:191	arg1	binding					212:218	initial binding	204:218	initial binding of IAV to sialylated glycans	204:247	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32298636	4	12	theme	target	871:876	arg1	vesicles					878:885	target vesicles	871:885	target vesicles	871:885	Here, we develop a fusion assay that uses synthetic DNA-lipid conjugates as surrogate viral receptors to tether virions to target vesicles.
32298636	3	13	theme	cholesterol	691:701	arg1	role					683:686	the role	679:686	the role of cholesterol in IAV fusion	679:715	These properties affect both IAV binding and fusion, which makes it difficult to isolate the role of cholesterol in IAV fusion from receptor binding effects.
32298636	3	14	theme	binding	731:737	arg1	effects					739:745	receptor binding effects	722:745	receptor binding effects	722:745	These properties affect both IAV binding and fusion, which makes it difficult to isolate the role of cholesterol in IAV fusion from receptor binding effects.
32298636	1	15	theme	viral	360:364	arg1	genome					366:371	the viral genome	356:371	the viral genome	356:371	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32298636	8	16	theme	mixing	1784:1789	arg1	efficiency					1791:1800	lipid mixing efficiency	1778:1800	lipid mixing efficiency	1778:1800	These results are consistent with the hypothesis that target membrane cholesterol increases lipid mixing efficiency by altering host membrane curvature.
32298636	7	17	theme	target	1624:1629	arg1	membrane					1631:1638	the target membrane	1620:1638	the target membrane	1620:1638	We also find that the single-particle kinetics of influenza lipid mixing to target membranes with different cholesterol compositions is independent of receptor binding, suggesting that cholesterol-mediated spatial clustering of viral receptors within the target membrane does not significantly affect IAV hemifusion.
32298636	5	18	theme	fusion	1146:1151	arg1	events					1153:1158	individual, pH-triggered IAV membrane fusion events	1108:1158	individual, pH-triggered IAV membrane fusion events	1108:1158	To avoid the possibly perturbative effect of adding a self-quenched concentration of dye-labeled lipids to the viral membrane, we tether virions to lipid-labeled target vesicles and use fluorescence microscopy to detect individual, pH-triggered IAV membrane fusion events.
32298636	6	19	theme	lipid	1279:1283	arg1	mixing					1285:1290	single-particle IAV lipid mixing	1259:1290	single-particle IAV lipid mixing	1259:1290	Through this approach, we find that cholesterol in the target membrane enhances the efficiency of single-particle IAV lipid mixing, whereas the rate of lipid mixing is independent of cholesterol composition.
32298636	5	20	theme	viral	999:1003	arg1	membrane					1005:1012	the viral membrane	995:1012	the viral membrane	995:1012	To avoid the possibly perturbative effect of adding a self-quenched concentration of dye-labeled lipids to the viral membrane, we tether virions to lipid-labeled target vesicles and use fluorescence microscopy to detect individual, pH-triggered IAV membrane fusion events.
32298636	6	21	theme	single-particle	1259:1273	arg1	mixing					1285:1290	single-particle IAV lipid mixing	1259:1290	single-particle IAV lipid mixing	1259:1290	Through this approach, we find that cholesterol in the target membrane enhances the efficiency of single-particle IAV lipid mixing, whereas the rate of lipid mixing is independent of cholesterol composition.
32298636	8	22	theme	host	1814:1817	arg1	curvature					1828:1836	host membrane curvature	1814:1836	host membrane curvature	1814:1836	These results are consistent with the hypothesis that target membrane cholesterol increases lipid mixing efficiency by altering host membrane curvature.
32298636	7	23	theme	viral	1597:1601	arg1	receptors					1603:1611	viral receptors	1597:1611	viral receptors	1597:1611	We also find that the single-particle kinetics of influenza lipid mixing to target membranes with different cholesterol compositions is independent of receptor binding, suggesting that cholesterol-mediated spatial clustering of viral receptors within the target membrane does not significantly affect IAV hemifusion.
32298636	6	24	theme	cholesterol	1344:1354	arg1	composition					1356:1366	cholesterol composition	1344:1366	cholesterol composition	1344:1366	Through this approach, we find that cholesterol in the target membrane enhances the efficiency of single-particle IAV lipid mixing, whereas the rate of lipid mixing is independent of cholesterol composition.
32298636	5	25	theme	fluorescence	1074:1085	arg1	microscopy					1087:1096	fluorescence microscopy	1074:1096	fluorescence microscopy	1074:1096	To avoid the possibly perturbative effect of adding a self-quenched concentration of dye-labeled lipids to the viral membrane, we tether virions to lipid-labeled target vesicles and use fluorescence microscopy to detect individual, pH-triggered IAV membrane fusion events.
32298636	1	26	theme	A	165:165	arg1	IAV					174:176	IAV	174:176	IAV	174:176	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32298636	1	26	theme	A	165:165	arg1	virus					167:171	the influenza A virus	151:171	the influenza A virus (IAV) entry process	151:191	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32298636	2	27	theme	membrane	552:559	arg1	properties					534:543	physical properties	525:543	physical properties of the membrane like fluidity and curvature	525:587	In particular, target membrane cholesterol has been shown to preferentially associate with virus receptors and alter physical properties of the membrane like fluidity and curvature.
32298636	7	28	theme	cholesterol-mediated	1554:1573	arg1	clustering					1583:1592	cholesterol-mediated spatial clustering	1554:1592	cholesterol-mediated spatial clustering of viral receptors within the target membrane	1554:1638	We also find that the single-particle kinetics of influenza lipid mixing to target membranes with different cholesterol compositions is independent of receptor binding, suggesting that cholesterol-mediated spatial clustering of viral receptors within the target membrane does not significantly affect IAV hemifusion.
32298636	6	29	theme	mixing	1319:1324	arg1	independent					1329:1339	independent	1329:1339	independent	1329:1339	Through this approach, we find that cholesterol in the target membrane enhances the efficiency of single-particle IAV lipid mixing, whereas the rate of lipid mixing is independent of cholesterol composition.
32298636	6	29	theme	mixing	1319:1324	arg1	rate					1305:1308	the rate	1301:1308	the rate of lipid mixing	1301:1324	Through this approach, we find that cholesterol in the target membrane enhances the efficiency of single-particle IAV lipid mixing, whereas the rate of lipid mixing is independent of cholesterol composition.
32298636	5	30	theme	target	1050:1055	arg1	vesicles					1057:1064	lipid-labeled target vesicles	1036:1064	lipid-labeled target vesicles	1036:1064	To avoid the possibly perturbative effect of adding a self-quenched concentration of dye-labeled lipids to the viral membrane, we tether virions to lipid-labeled target vesicles and use fluorescence microscopy to detect individual, pH-triggered IAV membrane fusion events.
32298636	1	31	gly	sialylated	230:239	arg1	glycans					241:247	sialylated glycans	230:247	sialylated glycans	230:247	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32298636	1	32	theme	pore	337:340	arg1	formation					315:323	the formation	311:323	the formation of a fusion pore through which the viral genome is transferred into a target cell	311:405	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32298636	1	32	theme	pore	337:340	arg1	fusion					250:255	fusion	250:255	fusion between the viral envelope and the host membrane	250:304	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32298636	1	32	theme	pore	337:340	arg1	binding					212:218	initial binding	204:218	initial binding of IAV to sialylated glycans	204:247	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32298636	0	33	theme	Efficiency	77:86	arg1	but Not Rate					88:99	Single Influenza Virus Membrane Fusion Efficiency but Not Rate	38:99	Single Influenza Virus Membrane Fusion Efficiency but Not Rate	38:99	Target Membrane Cholesterol Modulates Single Influenza Virus Membrane Fusion Efficiency but Not Rate.
32298636	0	34	theme	Target	0:5	arg1	Cholesterol					16:26	Target Membrane Cholesterol	0:26	Target Membrane Cholesterol	0:26	Target Membrane Cholesterol Modulates Single Influenza Virus Membrane Fusion Efficiency but Not Rate.
32298636	5	35	theme	membrane	1137:1144	arg1	events					1153:1158	individual, pH-triggered IAV membrane fusion events	1108:1158	individual, pH-triggered IAV membrane fusion events	1108:1158	To avoid the possibly perturbative effect of adding a self-quenched concentration of dye-labeled lipids to the viral membrane, we tether virions to lipid-labeled target vesicles and use fluorescence microscopy to detect individual, pH-triggered IAV membrane fusion events.
32298636	1	36	theme	lipid	107:111	arg1	composition					113:123	Host lipid composition	102:123	Host lipid composition	102:123	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32298636	7	37	theme	receptor	1520:1527	arg1	binding					1529:1535	receptor binding	1520:1535	receptor binding	1520:1535	We also find that the single-particle kinetics of influenza lipid mixing to target membranes with different cholesterol compositions is independent of receptor binding, suggesting that cholesterol-mediated spatial clustering of viral receptors within the target membrane does not significantly affect IAV hemifusion.
32298636	2	38	theme	membrane	430:437	arg1	cholesterol					439:449	target membrane cholesterol	423:449	target membrane cholesterol	423:449	In particular, target membrane cholesterol has been shown to preferentially associate with virus receptors and alter physical properties of the membrane like fluidity and curvature.
32298636	0	39	theme	Single	38:43	arg1	but Not Rate					88:99	Single Influenza Virus Membrane Fusion Efficiency but Not Rate	38:99	Single Influenza Virus Membrane Fusion Efficiency but Not Rate	38:99	Target Membrane Cholesterol Modulates Single Influenza Virus Membrane Fusion Efficiency but Not Rate.
32298636	5	40	theme	individual	1108:1117	arg1	events					1153:1158	individual, pH-triggered IAV membrane fusion events	1108:1158	individual, pH-triggered IAV membrane fusion events	1108:1158	To avoid the possibly perturbative effect of adding a self-quenched concentration of dye-labeled lipids to the viral membrane, we tether virions to lipid-labeled target vesicles and use fluorescence microscopy to detect individual, pH-triggered IAV membrane fusion events.
32298636	5	41	theme	lipids	985:990	arg1	concentration					956:968	a self-quenched concentration	940:968	a self-quenched concentration of dye-labeled lipids to the viral membrane	940:1012	To avoid the possibly perturbative effect of adding a self-quenched concentration of dye-labeled lipids to the viral membrane, we tether virions to lipid-labeled target vesicles and use fluorescence microscopy to detect individual, pH-triggered IAV membrane fusion events.
32298636	8	42	with	consistent	1704:1713	arg1	hypothesis					1724:1733	the hypothesis that target membrane cholesterol increases lipid mixing efficiency by altering host membrane curvature	1720:1836	the hypothesis that target membrane cholesterol increases lipid mixing efficiency by altering host membrane curvature	1720:1836	These results are consistent with the hypothesis that target membrane cholesterol increases lipid mixing efficiency by altering host membrane curvature.
32298636	0	43	theme	Fusion	70:75	arg1	but Not Rate					88:99	Single Influenza Virus Membrane Fusion Efficiency but Not Rate	38:99	Single Influenza Virus Membrane Fusion Efficiency but Not Rate	38:99	Target Membrane Cholesterol Modulates Single Influenza Virus Membrane Fusion Efficiency but Not Rate.
32298636	0	44	theme	Virus	55:59	arg1	but Not Rate					88:99	Single Influenza Virus Membrane Fusion Efficiency but Not Rate	38:99	Single Influenza Virus Membrane Fusion Efficiency but Not Rate	38:99	Target Membrane Cholesterol Modulates Single Influenza Virus Membrane Fusion Efficiency but Not Rate.
32298636	7	45	theme	different	1467:1475	arg1	compositions					1489:1500	different cholesterol compositions	1467:1500	different cholesterol compositions	1467:1500	We also find that the single-particle kinetics of influenza lipid mixing to target membranes with different cholesterol compositions is independent of receptor binding, suggesting that cholesterol-mediated spatial clustering of viral receptors within the target membrane does not significantly affect IAV hemifusion.
32298636	5	46	theme	self-quenched	942:954	arg1	concentration					956:968	a self-quenched concentration	940:968	a self-quenched concentration of dye-labeled lipids to the viral membrane	940:1012	To avoid the possibly perturbative effect of adding a self-quenched concentration of dye-labeled lipids to the viral membrane, we tether virions to lipid-labeled target vesicles and use fluorescence microscopy to detect individual, pH-triggered IAV membrane fusion events.
32298636	7	47	theme	lipid	1429:1433	arg1	mixing					1435:1440	influenza lipid mixing	1419:1440	influenza lipid mixing	1419:1440	We also find that the single-particle kinetics of influenza lipid mixing to target membranes with different cholesterol compositions is independent of receptor binding, suggesting that cholesterol-mediated spatial clustering of viral receptors within the target membrane does not significantly affect IAV hemifusion.
32298636	4	48	theme	surrogate	824:832	arg1	receptors					840:848	surrogate viral receptors	824:848	surrogate viral receptors	824:848	Here, we develop a fusion assay that uses synthetic DNA-lipid conjugates as surrogate viral receptors to tether virions to target vesicles.
32298636	1	49	theme	entry	179:183	arg1	process					185:191	the influenza A virus (IAV) entry process	151:191	the influenza A virus (IAV) entry process	151:191	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32298636	4	50	theme	synthetic	790:798	arg1	conjugates					810:819	synthetic DNA-lipid conjugates	790:819	synthetic DNA-lipid conjugates as surrogate viral receptors	790:848	Here, we develop a fusion assay that uses synthetic DNA-lipid conjugates as surrogate viral receptors to tether virions to target vesicles.
32298636	3	51	from	role	683:686	arg1	fusion					710:715	IAV fusion	706:715	IAV fusion	706:715	These properties affect both IAV binding and fusion, which makes it difficult to isolate the role of cholesterol in IAV fusion from receptor binding effects.
32298636	8	52	theme	target	1740:1745	arg1	cholesterol					1756:1766	target membrane cholesterol	1740:1766	target membrane cholesterol	1740:1766	These results are consistent with the hypothesis that target membrane cholesterol increases lipid mixing efficiency by altering host membrane curvature.
32298636	1	53	theme	initial	204:210	arg1	binding					212:218	initial binding	204:218	initial binding of IAV to sialylated glycans	204:247	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32298636	2	54	theme	virus	499:503	arg1	receptors					505:513	virus receptors	499:513	virus receptors	499:513	In particular, target membrane cholesterol has been shown to preferentially associate with virus receptors and alter physical properties of the membrane like fluidity and curvature.
32298636	6	55	theme	mixing	1285:1290	arg1	efficiency					1245:1254	the efficiency	1241:1254	the efficiency of single-particle IAV lipid mixing	1241:1290	Through this approach, we find that cholesterol in the target membrane enhances the efficiency of single-particle IAV lipid mixing, whereas the rate of lipid mixing is independent of cholesterol composition.
32298636	5	56	theme	lipid-labeled	1036:1048	arg1	vesicles					1057:1064	lipid-labeled target vesicles	1036:1064	lipid-labeled target vesicles	1036:1064	To avoid the possibly perturbative effect of adding a self-quenched concentration of dye-labeled lipids to the viral membrane, we tether virions to lipid-labeled target vesicles and use fluorescence microscopy to detect individual, pH-triggered IAV membrane fusion events.
32298636	8	57	theme	lipid	1778:1782	arg1	efficiency					1791:1800	lipid mixing efficiency	1778:1800	lipid mixing efficiency	1778:1800	These results are consistent with the hypothesis that target membrane cholesterol increases lipid mixing efficiency by altering host membrane curvature.
32298636	1	58	theme	many	136:139	arg1	formation					315:323	the formation	311:323	the formation of a fusion pore through which the viral genome is transferred into a target cell	311:405	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32298636	1	58	theme	many	136:139	arg1	fusion					250:255	fusion	250:255	fusion between the viral envelope and the host membrane	250:304	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32298636	1	58	theme	many	136:139	arg1	stages					141:146	many stages	136:146	many stages	136:146	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32298636	1	58	theme	many	136:139	arg1	binding					212:218	initial binding	204:218	initial binding of IAV to sialylated glycans	204:247	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32298636	7	59	theme	receptors	1603:1611	arg1	clustering					1583:1592	cholesterol-mediated spatial clustering	1554:1592	cholesterol-mediated spatial clustering of viral receptors within the target membrane	1554:1638	We also find that the single-particle kinetics of influenza lipid mixing to target membranes with different cholesterol compositions is independent of receptor binding, suggesting that cholesterol-mediated spatial clustering of viral receptors within the target membrane does not significantly affect IAV hemifusion.
32298636	3	60	theme	receptor	722:729	arg1	effects					739:745	receptor binding effects	722:745	receptor binding effects	722:745	These properties affect both IAV binding and fusion, which makes it difficult to isolate the role of cholesterol in IAV fusion from receptor binding effects.
32298636	6	61	theme	IAV	1275:1277	arg1	mixing					1285:1290	single-particle IAV lipid mixing	1259:1290	single-particle IAV lipid mixing	1259:1290	Through this approach, we find that cholesterol in the target membrane enhances the efficiency of single-particle IAV lipid mixing, whereas the rate of lipid mixing is independent of cholesterol composition.
32298636	2	62	theme	physical	525:532	arg1	properties					534:543	physical properties	525:543	physical properties of the membrane like fluidity and curvature	525:587	In particular, target membrane cholesterol has been shown to preferentially associate with virus receptors and alter physical properties of the membrane like fluidity and curvature.
32298636	6	63	from	cholesterol	1197:1207	arg1	membrane					1223:1230	the target membrane	1212:1230	the target membrane	1212:1230	Through this approach, we find that cholesterol in the target membrane enhances the efficiency of single-particle IAV lipid mixing, whereas the rate of lipid mixing is independent of cholesterol composition.
32298636	6	64	theme	composition	1356:1366	arg1	independent					1329:1339	independent	1329:1339	independent	1329:1339	Through this approach, we find that cholesterol in the target membrane enhances the efficiency of single-particle IAV lipid mixing, whereas the rate of lipid mixing is independent of cholesterol composition.
32298636	6	64	theme	composition	1356:1366	arg1	rate					1305:1308	the rate	1301:1308	the rate of lipid mixing	1301:1324	Through this approach, we find that cholesterol in the target membrane enhances the efficiency of single-particle IAV lipid mixing, whereas the rate of lipid mixing is independent of cholesterol composition.
32298636	1	65	theme	influenza	155:163	arg1	IAV					174:176	IAV	174:176	IAV	174:176	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32298636	1	65	theme	influenza	155:163	arg1	virus					167:171	the influenza A virus	151:171	the influenza A virus (IAV) entry process	151:191	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32298636	7	66	theme	spatial	1575:1581	arg1	clustering					1583:1592	cholesterol-mediated spatial clustering	1554:1592	cholesterol-mediated spatial clustering of viral receptors within the target membrane	1554:1638	We also find that the single-particle kinetics of influenza lipid mixing to target membranes with different cholesterol compositions is independent of receptor binding, suggesting that cholesterol-mediated spatial clustering of viral receptors within the target membrane does not significantly affect IAV hemifusion.
32298636	8	67	theme	membrane	1819:1826	arg1	curvature					1828:1836	host membrane curvature	1814:1836	host membrane curvature	1814:1836	These results are consistent with the hypothesis that target membrane cholesterol increases lipid mixing efficiency by altering host membrane curvature.
32298636	1	68	theme	virus	167:171	arg1	process					185:191	the influenza A virus (IAV) entry process	151:191	the influenza A virus (IAV) entry process	151:191	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32298636	5	69	theme	perturbative	910:921	arg1	effect					923:928	the possibly perturbative effect	897:928	the possibly perturbative effect of adding a self-quenched concentration of dye-labeled lipids to the viral membrane	897:1012	To avoid the possibly perturbative effect of adding a self-quenched concentration of dye-labeled lipids to the viral membrane, we tether virions to lipid-labeled target vesicles and use fluorescence microscopy to detect individual, pH-triggered IAV membrane fusion events.
32298636	6	70	theme	lipid	1313:1317	arg1	mixing					1319:1324	lipid mixing	1313:1324	lipid mixing	1313:1324	Through this approach, we find that cholesterol in the target membrane enhances the efficiency of single-particle IAV lipid mixing, whereas the rate of lipid mixing is independent of cholesterol composition.
32298636	1	71	theme	fusion	330:335	arg1	pore					337:340	a fusion pore	328:340	a fusion pore through which the viral genome is transferred into a target cell	328:405	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32298636	5	72	dep	individual	1108:1117	arg1	pH-triggered					1120:1131	pH-triggered	1120:1131	pH-triggered	1120:1131	To avoid the possibly perturbative effect of adding a self-quenched concentration of dye-labeled lipids to the viral membrane, we tether virions to lipid-labeled target vesicles and use fluorescence microscopy to detect individual, pH-triggered IAV membrane fusion events.
32298636	0	73	theme	Membrane	7:14	arg1	Cholesterol					16:26	Target Membrane Cholesterol	0:26	Target Membrane Cholesterol	0:26	Target Membrane Cholesterol Modulates Single Influenza Virus Membrane Fusion Efficiency but Not Rate.
32298636	1	74	theme	Host	102:105	arg1	composition					113:123	Host lipid composition	102:123	Host lipid composition	102:123	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32298636	1	75	theme	viral	269:273	arg1	envelope					275:282	the viral envelope	265:282	the viral envelope	265:282	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32298636	3	76	theme	IAV	619:621	arg1	binding					623:629	IAV binding	619:629	IAV binding	619:629	These properties affect both IAV binding and fusion, which makes it difficult to isolate the role of cholesterol in IAV fusion from receptor binding effects.
32298636	0	77	theme	Influenza	45:53	arg1	but Not Rate					88:99	Single Influenza Virus Membrane Fusion Efficiency but Not Rate	38:99	Single Influenza Virus Membrane Fusion Efficiency but Not Rate	38:99	Target Membrane Cholesterol Modulates Single Influenza Virus Membrane Fusion Efficiency but Not Rate.
32298636	7	78	theme	binding	1529:1535	arg1	independent					1505:1515	independent	1505:1515	independent	1505:1515	We also find that the single-particle kinetics of influenza lipid mixing to target membranes with different cholesterol compositions is independent of receptor binding, suggesting that cholesterol-mediated spatial clustering of viral receptors within the target membrane does not significantly affect IAV hemifusion.
32298636	7	78	theme	binding	1529:1535	arg1	kinetics					1407:1414	the single-particle kinetics	1387:1414	the single-particle kinetics of influenza lipid mixing to target membranes with different cholesterol compositions	1387:1500	We also find that the single-particle kinetics of influenza lipid mixing to target membranes with different cholesterol compositions is independent of receptor binding, suggesting that cholesterol-mediated spatial clustering of viral receptors within the target membrane does not significantly affect IAV hemifusion.
32298636	5	79	theme	dye-labeled	973:983	arg1	lipids					985:990	dye-labeled lipids	973:990	dye-labeled lipids	973:990	To avoid the possibly perturbative effect of adding a self-quenched concentration of dye-labeled lipids to the viral membrane, we tether virions to lipid-labeled target vesicles and use fluorescence microscopy to detect individual, pH-triggered IAV membrane fusion events.
32298636	7	80	theme	IAV	1670:1672	arg1	hemifusion					1674:1683	IAV hemifusion	1670:1683	IAV hemifusion	1670:1683	We also find that the single-particle kinetics of influenza lipid mixing to target membranes with different cholesterol compositions is independent of receptor binding, suggesting that cholesterol-mediated spatial clustering of viral receptors within the target membrane does not significantly affect IAV hemifusion.
32298636	2	81	theme	target	423:428	arg1	cholesterol					439:449	target membrane cholesterol	423:449	target membrane cholesterol	423:449	In particular, target membrane cholesterol has been shown to preferentially associate with virus receptors and alter physical properties of the membrane like fluidity and curvature.
32298636	0	82	theme	Membrane	61:68	arg1	but Not Rate					88:99	Single Influenza Virus Membrane Fusion Efficiency but Not Rate	38:99	Single Influenza Virus Membrane Fusion Efficiency but Not Rate	38:99	Target Membrane Cholesterol Modulates Single Influenza Virus Membrane Fusion Efficiency but Not Rate.
32298636	1	83	theme	host	292:295	arg1	membrane					297:304	the host membrane	288:304	the host membrane	288:304	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32298636	4	84	theme	fusion	767:772	arg1	assay					774:778	a fusion assay	765:778	a fusion assay that uses synthetic DNA-lipid conjugates as surrogate viral receptors to tether virions to target vesicles	765:885	Here, we develop a fusion assay that uses synthetic DNA-lipid conjugates as surrogate viral receptors to tether virions to target vesicles.
32298636	5	85	theme	IAV	1133:1135	arg1	events					1153:1158	individual, pH-triggered IAV membrane fusion events	1108:1158	individual, pH-triggered IAV membrane fusion events	1108:1158	To avoid the possibly perturbative effect of adding a self-quenched concentration of dye-labeled lipids to the viral membrane, we tether virions to lipid-labeled target vesicles and use fluorescence microscopy to detect individual, pH-triggered IAV membrane fusion events.
32298636	1	86	theme	IAV	223:225	arg1	formation					315:323	the formation	311:323	the formation of a fusion pore through which the viral genome is transferred into a target cell	311:405	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32298636	1	86	theme	IAV	223:225	arg1	fusion					250:255	fusion	250:255	fusion between the viral envelope and the host membrane	250:304	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32298636	1	86	theme	IAV	223:225	arg1	binding					212:218	initial binding	204:218	initial binding of IAV to sialylated glycans	204:247	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32298636	7	87	theme	cholesterol	1477:1487	arg1	compositions					1489:1500	different cholesterol compositions	1467:1500	different cholesterol compositions	1467:1500	We also find that the single-particle kinetics of influenza lipid mixing to target membranes with different cholesterol compositions is independent of receptor binding, suggesting that cholesterol-mediated spatial clustering of viral receptors within the target membrane does not significantly affect IAV hemifusion.
32298636	1	88	theme	sialylated	230:239	arg1	glycans					241:247	sialylated glycans	230:247	sialylated glycans	230:247	Host lipid composition influences many stages of the influenza A virus (IAV) entry process, including initial binding of IAV to sialylated glycans, fusion between the viral envelope and the host membrane, and the formation of a fusion pore through which the viral genome is transferred into a target cell.
32220841	6	0	theme	ruminant	919:926	arg1	milks					928:932	ruminant milks	919:932	ruminant milks	919:932	Formula milks often contain galacto- and/or fructo-oligosaccharides (GOS and FOS, respectively) as additives to augment the concentration of oligosaccharides in ruminant milks, but the ability of B. longum subsp.
32220841	19	1	theme	various	2541:2547	arg1	species					2565:2571	various bifidobacterial species	2541:2571	various bifidobacterial species	2541:2571	However, studies of the ability of various bifidobacterial species to grow together in the presence of these oligosaccharides have not been conducted.
32220841	11	2	dep	DP	1501:1502	arg1	to					1506:1507	to	1506:1507	to	1506:1507	infantis had the ability to utilize both short- and longer-chain GOS and FOS (DP 2 to 6).
32220841	17	3	theme	well-known	2261:2270	arg1	species					2282:2288	a well-known bacterial species	2259:2288	a well-known bacterial species	2259:2288	Bifidobacterium longum subspecies infantis is a well-known bacterial species, but under modern child-rearing conditions it may be disadvantaged in the gut.
32220841	17	3	theme	well-known	2261:2270	arg1	longum					2229:2234	Bifidobacterium longum	2213:2234	Bifidobacterium longum	2213:2234	Bifidobacterium longum subspecies infantis is a well-known bacterial species, but under modern child-rearing conditions it may be disadvantaged in the gut.
32220841	6	4	theme	oligosaccharides	899:914	arg1	concentration					882:894	the concentration	878:894	the concentration of oligosaccharides in ruminant milks	878:932	Formula milks often contain galacto- and/or fructo-oligosaccharides (GOS and FOS, respectively) as additives to augment the concentration of oligosaccharides in ruminant milks, but the ability of B. longum subsp.
32220841	15	5	theme	substrate	1727:1735	arg1	utilization					1737:1747	substrate utilization	1727:1747	substrate utilization	1727:1747	infantis, indicating that the kinetics of substrate utilization is an important ecological factor in the assemblage of gut communities.IMPORTANCE The kinds of bacteria that form the collection of microbes (the microbiota) in the gut of human infants may influence health and well-being.
32220841	5	6	theme	infant	664:669	arg1	diet					671:674	the infant diet	660:674	the infant diet	660:674	Supplementation of the infant diet or replacement of breast milk using formula feeds is common in Western countries.
32220841	11	7	theme	longer-chain	1475:1486	arg1	GOS					1488:1490	both short- and longer-chain GOS	1459:1490	both short- and longer-chain GOS	1459:1490	infantis had the ability to utilize both short- and longer-chain GOS and FOS (DP 2 to 6).
32220841	1	8	theme	diet	263:266	arg1	milk					230:233	milk	230:233	milk	230:233	Bifidobacterial species are common inhabitants of the gut of human infants during the period when milk is a major component of the diet.
32220841	1	8	theme	diet	263:266	arg1	component					246:254	a major component	238:254	a major component of the diet	238:266	Bifidobacterial species are common inhabitants of the gut of human infants during the period when milk is a major component of the diet.
32220841	20	9	theme	microbiota	2813:2822	arg1	development					2790:2800	the development	2786:2800	the development of the gut microbiota	2786:2822	These kinds of studies are essential for developing concepts of microbial ecology related to the influence of human nutrition on the development of the gut microbiota.
32220841	6	10	dep	milks	766:770	arg1	contain					778:784	contain	778:784	milks often contain galacto- and/or fructo-oligosaccharides (GOS and FOS, respectively) as additives to augment the concentration of oligosaccharides in ruminant milks, but the ability of B. longum subsp	766:968	Formula milks often contain galacto- and/or fructo-oligosaccharides (GOS and FOS, respectively) as additives to augment the concentration of oligosaccharides in ruminant milks, but the ability of B. longum subsp.
32220841	6	11	from	concentration	882:894	arg1	milks					928:932	ruminant milks	919:932	ruminant milks	919:932	Formula milks often contain galacto- and/or fructo-oligosaccharides (GOS and FOS, respectively) as additives to augment the concentration of oligosaccharides in ruminant milks, but the ability of B. longum subsp.
32220841	14	12	theme	GOS	1653:1655	arg1	use					1646:1648	the slower use	1635:1648	the slower use of GOS of DP 3 by B. longum subsp	1635:1682	This was probably related to the slower use of GOS of DP 3 by B. longum subsp.
32220841	3	13	theme	westernized	528:538	arg1	countries					540:548	westernized countries	528:548	westernized countries	528:548	infantis may be disadvantaged numerically in the gut of infants in westernized countries.
32220841	5	14	from	countries	747:755	arg1	common					729:734	common	729:734	common	729:734	Supplementation of the infant diet or replacement of breast milk using formula feeds is common in Western countries.
32220841	20	15	from	influence	2754:2762	arg1	development					2790:2800	the development	2786:2800	the development of the gut microbiota	2786:2822	These kinds of studies are essential for developing concepts of microbial ecology related to the influence of human nutrition on the development of the gut microbiota.
32220841	15	16	theme	communities.IMPORTANCE	1808:1829	arg1	assemblage					1790:1799	the assemblage	1786:1799	the assemblage of gut communities.IMPORTANCE The kinds of bacteria that form the collection of microbes (the microbiota) in the gut of human infants may influence health and well-being	1786:1969	infantis, indicating that the kinetics of substrate utilization is an important ecological factor in the assemblage of gut communities.IMPORTANCE The kinds of bacteria that form the collection of microbes (the microbiota) in the gut of human infants may influence health and well-being.
32220841	1	17	theme	infants	199:205	arg1	gut					186:188	the gut	182:188	the gut of human infants during the period	182:223	Bifidobacterial species are common inhabitants of the gut of human infants during the period when milk is a major component of the diet.
32220841	8	18	theme	pure	1201:1204	arg1	culture					1206:1212	pure culture	1201:1212	pure culture	1201:1212	We compared the growth and oligosaccharide utilization of GOS and FOS by bifidobacterial species in pure culture and coculture.
32220841	4	19	theme	different	620:628	arg1	countries					630:638	different countries	620:638	different countries	620:638	This may be due to the different durations of breast milk feeding in different countries.
32220841	16	20	theme	diet	2019:2022	arg1	composition					1993:2003	the composition	1989:2003	the composition of the infant diet	1989:2022	Knowledge of how the composition of the infant diet influences the assemblage of the bacterial collection is therefore important because dietary interventions may offer opportunities to alter the microbiota with the aim of improving health.
32220841	7	21	theme	bifidobacterial	1065:1079	arg1	species					1081:1087	other bifidobacterial species	1059:1087	other bifidobacterial species	1059:1087	infantis to utilize these potential growth substrates when they are in competition with other bifidobacterial species is unknown.
32220841	9	22	theme	Short-chain	1229:1239	arg1	GOS					1241:1243	Short-chain GOS	1229:1243	Short-chain GOS	1229:1243	Short-chain GOS and FOS (degrees of polymerization [DP] 2 and 3) were favored growth substrates for strains of B. bifidum and B. longum subsp.
32220841	7	23	theme	growth	1007:1012	arg1	substrates					1014:1023	these potential growth substrates	991:1023	these potential growth substrates	991:1023	infantis to utilize these potential growth substrates when they are in competition with other bifidobacterial species is unknown.
32220841	18	24	theme	oligosaccharide	2415:2429	arg1	additives					2431:2439	particular oligosaccharide additives	2404:2439	particular oligosaccharide additives that are generally considered to support bifidobacterial growth	2404:2503	Modern formula milks often contain particular oligosaccharide additives that are generally considered to support bifidobacterial growth.
32220841	2	25	dep	bifidum	308:314	arg1	infantis					384:391	subspecies infantis	373:391	subspecies infantis	373:391	Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum subspecies longum, and B. longum subspecies infantis have been detected frequently in infant feces, but B. longum subsp.
32220841	20	26	theme	microbial	2721:2729	arg1	ecology					2731:2737	microbial ecology	2721:2737	microbial ecology	2721:2737	These kinds of studies are essential for developing concepts of microbial ecology related to the influence of human nutrition on the development of the gut microbiota.
32220841	1	27	theme	Bifidobacterial	132:146	arg1	species					148:154	Bifidobacterial species	132:154	Bifidobacterial species	132:154	Bifidobacterial species are common inhabitants of the gut of human infants during the period when milk is a major component of the diet.
32220841	1	27	theme	Bifidobacterial	132:146	arg1	inhabitants					167:177	common inhabitants	160:177	common inhabitants of the gut of human infants during the period	160:223	Bifidobacterial species are common inhabitants of the gut of human infants during the period when milk is a major component of the diet.
32220841	5	28	dep	feeds	720:724	arg1	common					729:734	common	729:734	common	729:734	Supplementation of the infant diet or replacement of breast milk using formula feeds is common in Western countries.
32220841	2	29	located	detected	403:410	arg2	breve					285:289	Bifidobacterium breve	269:289	Bifidobacterium breve	269:289	Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum subspecies longum, and B. longum subspecies infantis have been detected frequently in infant feces, but B. longum subsp.
32220841	2	29	located	detected	403:410	arg2	bifidum					308:314	Bifidobacterium bifidum	292:314	Bifidobacterium bifidum	292:314	Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum subspecies longum, and B. longum subspecies infantis have been detected frequently in infant feces, but B. longum subsp.
32220841	2	29	located	detected	403:410	arg2	longum					351:356	Bifidobacterium longum subspecies longum	317:356	Bifidobacterium longum subspecies longum	317:356	Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum subspecies longum, and B. longum subspecies infantis have been detected frequently in infant feces, but B. longum subsp.
32220841	2	29	located	detected	403:410	arg1	subsp					454:458	B. longum subsp	444:458	B. longum subsp	444:458	Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum subspecies longum, and B. longum subspecies infantis have been detected frequently in infant feces, but B. longum subsp.
32220841	2	29	located	detected	403:410	arg1	feces					433:437	infant feces	426:437	infant feces	426:437	Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum subspecies longum, and B. longum subspecies infantis have been detected frequently in infant feces, but B. longum subsp.
32220841	2	29	located	detected	403:410	arg2	longum					366:371	B. longum	363:371	B. longum	363:371	Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum subspecies longum, and B. longum subspecies infantis have been detected frequently in infant feces, but B. longum subsp.
32220841	18	30	contain	contain	2396:2402	arg2	additives					2431:2439	particular oligosaccharide additives	2404:2439	particular oligosaccharide additives that are generally considered to support bifidobacterial growth	2404:2503	Modern formula milks often contain particular oligosaccharide additives that are generally considered to support bifidobacterial growth.
32220841	18	30	contain	contain	2396:2402	arg1	milks					2384:2388	Modern formula milks	2369:2388	Modern formula milks	2369:2388	Modern formula milks often contain particular oligosaccharide additives that are generally considered to support bifidobacterial growth.
32220841	20	31	theme	related	2739:2745	arg1	concepts					2709:2716	concepts	2709:2716	concepts of microbial ecology related to the influence of human nutrition on the development of the gut microbiota	2709:2822	These kinds of studies are essential for developing concepts of microbial ecology related to the influence of human nutrition on the development of the gut microbiota.
32220841	9	32	theme	[DP	1280:1282	arg1	degrees					1254:1260	degrees	1254:1260	degrees of polymerization [DP	1254:1282	Short-chain GOS and FOS (degrees of polymerization [DP] 2 and 3) were favored growth substrates for strains of B. bifidum and B. longum subsp.
32220841	10	33	theme	B.	1406:1407	arg1	subsp					1416:1420	B. longum subsp	1406:1420	B. longum subsp	1406:1420	longum, whereas both B. breve and B. longum subsp.
32220841	0	34	theme	Species	90:96	arg1	Cocultures					60:69	Cocultures	60:69	Cocultures of Bifidobacterial Species Characteristic of the Infant Gut	60:129	Galacto- and Fructo-oligosaccharides Utilized for Growth by Cocultures of Bifidobacterial Species Characteristic of the Infant Gut.
32220841	19	35	theme	bifidobacterial	2549:2563	arg1	species					2565:2571	various bifidobacterial species	2541:2571	various bifidobacterial species	2541:2571	However, studies of the ability of various bifidobacterial species to grow together in the presence of these oligosaccharides have not been conducted.
32220841	0	36	theme	Infant	120:125	arg1	Gut					127:129	the Infant Gut	116:129	the Infant Gut	116:129	Galacto- and Fructo-oligosaccharides Utilized for Growth by Cocultures of Bifidobacterial Species Characteristic of the Infant Gut.
32220841	0	37	theme	Bifidobacterial	74:88	arg1	Species					90:96	Bifidobacterial Species	74:96	Bifidobacterial Species Characteristic of the Infant Gut	74:129	Galacto- and Fructo-oligosaccharides Utilized for Growth by Cocultures of Bifidobacterial Species Characteristic of the Infant Gut.
32220841	9	38	theme	growth	1307:1312	arg1	substrates					1314:1323	favored growth substrates	1299:1323	favored growth substrates for strains of B. bifidum and B. longum subsp	1299:1369	Short-chain GOS and FOS (degrees of polymerization [DP] 2 and 3) were favored growth substrates for strains of B. bifidum and B. longum subsp.
32220841	2	39	dep	B.	444:445	arg1	longum					447:452	longum	447:452	longum	447:452	Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum subspecies longum, and B. longum subspecies infantis have been detected frequently in infant feces, but B. longum subsp.
32220841	15	40	theme	infants	1927:1933	arg1	gut					1914:1916	the gut	1910:1916	the gut of human infants	1910:1933	infantis, indicating that the kinetics of substrate utilization is an important ecological factor in the assemblage of gut communities.IMPORTANCE The kinds of bacteria that form the collection of microbes (the microbiota) in the gut of human infants may influence health and well-being.
32220841	7	41	dep	infantis	971:978	arg1	utilize					983:989	utilize	983:989	to utilize these potential growth substrates when they are in competition with other bifidobacterial species	980:1087	infantis to utilize these potential growth substrates when they are in competition with other bifidobacterial species is unknown.
32220841	17	42	theme	child-rearing	2308:2320	arg1	conditions					2322:2331	modern child-rearing conditions	2301:2331	modern child-rearing conditions	2301:2331	Bifidobacterium longum subspecies infantis is a well-known bacterial species, but under modern child-rearing conditions it may be disadvantaged in the gut.
32220841	18	43	theme	bifidobacterial	2482:2496	arg1	growth					2498:2503	bifidobacterial growth	2482:2503	bifidobacterial growth	2482:2503	Modern formula milks often contain particular oligosaccharide additives that are generally considered to support bifidobacterial growth.
32220841	16	44	theme	dietary	2109:2115	arg1	interventions					2117:2129	dietary interventions	2109:2129	dietary interventions	2109:2129	Knowledge of how the composition of the infant diet influences the assemblage of the bacterial collection is therefore important because dietary interventions may offer opportunities to alter the microbiota with the aim of improving health.
32220841	12	45	dep	B.	1565:1566	arg1	longum					1568:1573	longum	1568:1573	longum	1568:1573	B. breve was nevertheless numerically dominant over B. longum subsp.
32220841	9	46	theme	bifidum	1343:1349	arg1	subsp					1365:1369	B. bifidum and B. longum subsp	1340:1369	B. bifidum and B. longum subsp	1340:1369	Short-chain GOS and FOS (degrees of polymerization [DP] 2 and 3) were favored growth substrates for strains of B. bifidum and B. longum subsp.
32220841	20	47	theme	ecology	2731:2737	arg1	concepts					2709:2716	concepts	2709:2716	concepts of microbial ecology related to the influence of human nutrition on the development of the gut microbiota	2709:2822	These kinds of studies are essential for developing concepts of microbial ecology related to the influence of human nutrition on the development of the gut microbiota.
32220841	18	48	theme	Modern	2369:2374	arg1	milks					2384:2388	Modern formula milks	2369:2388	Modern formula milks	2369:2388	Modern formula milks often contain particular oligosaccharide additives that are generally considered to support bifidobacterial growth.
32220841	9	49	dep	FOS	1249:1251	arg1	degrees					1254:1260	degrees	1254:1260	degrees of polymerization [DP	1254:1282	Short-chain GOS and FOS (degrees of polymerization [DP] 2 and 3) were favored growth substrates for strains of B. bifidum and B. longum subsp.
32220841	16	50	theme	collection	2067:2076	arg1	assemblage					2039:2048	the assemblage	2035:2048	the assemblage of the bacterial collection	2035:2076	Knowledge of how the composition of the infant diet influences the assemblage of the bacterial collection is therefore important because dietary interventions may offer opportunities to alter the microbiota with the aim of improving health.
32220841	14	51	theme	DP	1660:1661	arg1	GOS					1653:1655	GOS	1653:1655	GOS of DP 3	1653:1663	This was probably related to the slower use of GOS of DP 3 by B. longum subsp.
32220841	16	52	theme	infant	2012:2017	arg1	diet					2019:2022	the infant diet	2008:2022	the infant diet	2008:2022	Knowledge of how the composition of the infant diet influences the assemblage of the bacterial collection is therefore important because dietary interventions may offer opportunities to alter the microbiota with the aim of improving health.
32220841	6	53	from	milks	928:932	arg1	concentration					882:894	the concentration	878:894	the concentration of oligosaccharides in ruminant milks	878:932	Formula milks often contain galacto- and/or fructo-oligosaccharides (GOS and FOS, respectively) as additives to augment the concentration of oligosaccharides in ruminant milks, but the ability of B. longum subsp.
32220841	4	54	theme	milk	604:607	arg1	durations					584:592	the different durations	570:592	the different durations of breast milk feeding in different countries	570:638	This may be due to the different durations of breast milk feeding in different countries.
32220841	15	55	theme	utilization	1737:1747	arg1	kinetics					1715:1722	the kinetics	1711:1722	the kinetics of substrate utilization	1711:1747	infantis, indicating that the kinetics of substrate utilization is an important ecological factor in the assemblage of gut communities.IMPORTANCE The kinds of bacteria that form the collection of microbes (the microbiota) in the gut of human infants may influence health and well-being.
32220841	15	55	theme	utilization	1737:1747	arg1	factor					1776:1781	an important ecological factor	1752:1781	an important ecological factor in the assemblage of gut communities.IMPORTANCE The kinds of bacteria that form the collection of microbes (the microbiota) in the gut of human infants may influence health and well-being	1752:1969	infantis, indicating that the kinetics of substrate utilization is an important ecological factor in the assemblage of gut communities.IMPORTANCE The kinds of bacteria that form the collection of microbes (the microbiota) in the gut of human infants may influence health and well-being.
32220841	15	56	from	factor	1776:1781	arg1	assemblage					1790:1799	the assemblage	1786:1799	the assemblage of gut communities.IMPORTANCE The kinds of bacteria that form the collection of microbes (the microbiota) in the gut of human infants may influence health and well-being	1786:1969	infantis, indicating that the kinetics of substrate utilization is an important ecological factor in the assemblage of gut communities.IMPORTANCE The kinds of bacteria that form the collection of microbes (the microbiota) in the gut of human infants may influence health and well-being.
32220841	15	57	dep	assemblage	1790:1799	arg1	influence					1939:1947	influence	1939:1947	may influence health and well-being	1935:1969	infantis, indicating that the kinetics of substrate utilization is an important ecological factor in the assemblage of gut communities.IMPORTANCE The kinds of bacteria that form the collection of microbes (the microbiota) in the gut of human infants may influence health and well-being.
32220841	13	58	from	infantis	1582:1589	arg1	cocultures					1594:1603	cocultures	1594:1603	cocultures	1594:1603	infantis in cocultures.
32220841	8	59	from	species	1190:1196	arg1	coculture					1218:1226	coculture	1218:1226	coculture	1218:1226	We compared the growth and oligosaccharide utilization of GOS and FOS by bifidobacterial species in pure culture and coculture.
32220841	8	59	from	species	1190:1196	arg1	culture					1206:1212	pure culture	1201:1212	pure culture	1201:1212	We compared the growth and oligosaccharide utilization of GOS and FOS by bifidobacterial species in pure culture and coculture.
32220841	17	60	theme	bacterial	2272:2280	arg1	species					2282:2288	a well-known bacterial species	2259:2288	a well-known bacterial species	2259:2288	Bifidobacterium longum subspecies infantis is a well-known bacterial species, but under modern child-rearing conditions it may be disadvantaged in the gut.
32220841	17	60	theme	bacterial	2272:2280	arg1	longum					2229:2234	Bifidobacterium longum	2213:2234	Bifidobacterium longum	2213:2234	Bifidobacterium longum subspecies infantis is a well-known bacterial species, but under modern child-rearing conditions it may be disadvantaged in the gut.
32220841	3	61	from	gut	510:512	arg1	countries					540:548	westernized countries	528:548	westernized countries	528:548	infantis may be disadvantaged numerically in the gut of infants in westernized countries.
32220841	8	62	theme	GOS	1159:1161	arg1	utilization					1144:1154	the growth and oligosaccharide utilization	1113:1154	the growth and oligosaccharide utilization of GOS and FOS by bifidobacterial species in pure culture and coculture	1113:1226	We compared the growth and oligosaccharide utilization of GOS and FOS by bifidobacterial species in pure culture and coculture.
32220841	4	63	theme	different	574:582	arg1	durations					584:592	the different durations	570:592	the different durations of breast milk feeding in different countries	570:638	This may be due to the different durations of breast milk feeding in different countries.
32220841	20	64	theme	studies	2672:2678	arg1	kinds					2663:2667	These kinds	2657:2667	These kinds of studies	2657:2678	These kinds of studies are essential for developing concepts of microbial ecology related to the influence of human nutrition on the development of the gut microbiota.
32220841	2	65	theme	Bifidobacterium	269:283	arg1	breve					285:289	Bifidobacterium breve	269:289	Bifidobacterium breve	269:289	Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum subspecies longum, and B. longum subspecies infantis have been detected frequently in infant feces, but B. longum subsp.
32220841	2	65	theme	Bifidobacterium	269:283	arg1	bifidum					308:314	Bifidobacterium bifidum	292:314	Bifidobacterium bifidum	292:314	Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum subspecies longum, and B. longum subspecies infantis have been detected frequently in infant feces, but B. longum subsp.
32220841	2	65	theme	Bifidobacterium	269:283	arg1	longum					366:371	B. longum	363:371	B. longum	363:371	Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum subspecies longum, and B. longum subspecies infantis have been detected frequently in infant feces, but B. longum subsp.
32220841	2	65	theme	Bifidobacterium	269:283	arg1	longum					351:356	Bifidobacterium longum subspecies longum	317:356	Bifidobacterium longum subspecies longum	317:356	Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum subspecies longum, and B. longum subspecies infantis have been detected frequently in infant feces, but B. longum subsp.
32220841	1	66	theme	gut	186:188	arg1	species					148:154	Bifidobacterial species	132:154	Bifidobacterial species	132:154	Bifidobacterial species are common inhabitants of the gut of human infants during the period when milk is a major component of the diet.
32220841	1	66	theme	gut	186:188	arg1	inhabitants					167:177	common inhabitants	160:177	common inhabitants of the gut of human infants during the period	160:223	Bifidobacterial species are common inhabitants of the gut of human infants during the period when milk is a major component of the diet.
32220841	8	67	theme	FOS	1167:1169	arg1	utilization					1144:1154	the growth and oligosaccharide utilization	1113:1154	the growth and oligosaccharide utilization of GOS and FOS by bifidobacterial species in pure culture and coculture	1113:1226	We compared the growth and oligosaccharide utilization of GOS and FOS by bifidobacterial species in pure culture and coculture.
32220841	20	68	theme	gut	2809:2811	arg1	microbiota					2813:2822	the gut microbiota	2805:2822	the gut microbiota	2805:2822	These kinds of studies are essential for developing concepts of microbial ecology related to the influence of human nutrition on the development of the gut microbiota.
32220841	15	69	theme	gut	1804:1806	arg1	communities.IMPORTANCE					1808:1829	gut communities.IMPORTANCE	1804:1829	gut communities.IMPORTANCE	1804:1829	infantis, indicating that the kinetics of substrate utilization is an important ecological factor in the assemblage of gut communities.IMPORTANCE The kinds of bacteria that form the collection of microbes (the microbiota) in the gut of human infants may influence health and well-being.
32220841	1	70	theme	human	193:197	arg1	infants					199:205	human infants	193:205	human infants	193:205	Bifidobacterial species are common inhabitants of the gut of human infants during the period when milk is a major component of the diet.
32220841	8	71	theme	bifidobacterial	1174:1188	arg1	species					1190:1196	bifidobacterial species	1174:1196	bifidobacterial species in pure culture and coculture	1174:1226	We compared the growth and oligosaccharide utilization of GOS and FOS by bifidobacterial species in pure culture and coculture.
32220841	6	72	theme	subsp	964:968	arg1	ability					943:949	the ability	939:949	the ability of B. longum subsp	939:968	Formula milks often contain galacto- and/or fructo-oligosaccharides (GOS and FOS, respectively) as additives to augment the concentration of oligosaccharides in ruminant milks, but the ability of B. longum subsp.
32220841	16	73	theme	bacterial	2057:2065	arg1	collection					2067:2076	the bacterial collection	2053:2076	the bacterial collection	2053:2076	Knowledge of how the composition of the infant diet influences the assemblage of the bacterial collection is therefore important because dietary interventions may offer opportunities to alter the microbiota with the aim of improving health.
32220841	20	74	theme	nutrition	2773:2781	arg1	influence					2754:2762	the influence	2750:2762	the influence of human nutrition on the development of the gut microbiota	2750:2822	These kinds of studies are essential for developing concepts of microbial ecology related to the influence of human nutrition on the development of the gut microbiota.
32220841	6	75	dep	fructo-oligosaccharides	802:824	arg1	FOS					835:837	FOS	835:837	FOS	835:837	Formula milks often contain galacto- and/or fructo-oligosaccharides (GOS and FOS, respectively) as additives to augment the concentration of oligosaccharides in ruminant milks, but the ability of B. longum subsp.
32220841	6	75	dep	fructo-oligosaccharides	802:824	arg1	GOS					827:829	GOS	827:829	GOS	827:829	Formula milks often contain galacto- and/or fructo-oligosaccharides (GOS and FOS, respectively) as additives to augment the concentration of oligosaccharides in ruminant milks, but the ability of B. longum subsp.
32220841	14	76	dep	B.	1668:1669	arg1	longum					1671:1676	longum	1671:1676	longum	1671:1676	This was probably related to the slower use of GOS of DP 3 by B. longum subsp.
32220841	5	77	theme	breast	694:699	arg1	milk					701:704	breast milk	694:704	breast milk	694:704	Supplementation of the infant diet or replacement of breast milk using formula feeds is common in Western countries.
32220841	6	78	theme	B.	954:955	arg1	subsp					964:968	B. longum subsp	954:968	B. longum subsp	954:968	Formula milks often contain galacto- and/or fructo-oligosaccharides (GOS and FOS, respectively) as additives to augment the concentration of oligosaccharides in ruminant milks, but the ability of B. longum subsp.
32220841	20	79	theme	human	2767:2771	arg1	nutrition					2773:2781	human nutrition	2767:2781	human nutrition	2767:2781	These kinds of studies are essential for developing concepts of microbial ecology related to the influence of human nutrition on the development of the gut microbiota.
32220841	15	80	theme	ecological	1765:1774	arg1	factor					1776:1781	an important ecological factor	1752:1781	an important ecological factor in the assemblage of gut communities.IMPORTANCE The kinds of bacteria that form the collection of microbes (the microbiota) in the gut of human infants may influence health and well-being	1752:1969	infantis, indicating that the kinetics of substrate utilization is an important ecological factor in the assemblage of gut communities.IMPORTANCE The kinds of bacteria that form the collection of microbes (the microbiota) in the gut of human infants may influence health and well-being.
32220841	15	80	theme	ecological	1765:1774	arg1	kinetics					1715:1722	the kinetics	1711:1722	the kinetics of substrate utilization	1711:1747	infantis, indicating that the kinetics of substrate utilization is an important ecological factor in the assemblage of gut communities.IMPORTANCE The kinds of bacteria that form the collection of microbes (the microbiota) in the gut of human infants may influence health and well-being.
32220841	5	81	theme	diet	671:674	arg1	Supplementation					641:655	Supplementation	641:655	Supplementation of the infant diet or replacement of breast milk using formula	641:718	Supplementation of the infant diet or replacement of breast milk using formula feeds is common in Western countries.
32220841	12	82	theme	B.	1513:1514	arg1	breve					1516:1520	B. breve	1513:1520	B. breve	1513:1520	B. breve was nevertheless numerically dominant over B. longum subsp.
32220841	7	83	theme	other	1059:1063	arg1	species					1081:1087	other bifidobacterial species	1059:1087	other bifidobacterial species	1059:1087	infantis to utilize these potential growth substrates when they are in competition with other bifidobacterial species is unknown.
32220841	6	84	dep	B.	954:955	arg1	longum					957:962	longum	957:962	longum	957:962	Formula milks often contain galacto- and/or fructo-oligosaccharides (GOS and FOS, respectively) as additives to augment the concentration of oligosaccharides in ruminant milks, but the ability of B. longum subsp.
32220841	5	85	theme	replacement	679:689	arg1	Supplementation					641:655	Supplementation	641:655	Supplementation of the infant diet or replacement of breast milk using formula	641:718	Supplementation of the infant diet or replacement of breast milk using formula feeds is common in Western countries.
32220841	7	86	theme	potential	997:1005	arg1	substrates					1014:1023	these potential growth substrates	991:1023	these potential growth substrates	991:1023	infantis to utilize these potential growth substrates when they are in competition with other bifidobacterial species is unknown.
32220841	5	87	theme	Western	739:745	arg1	countries					747:755	Western countries	739:755	Western countries	739:755	Supplementation of the infant diet or replacement of breast milk using formula feeds is common in Western countries.
32220841	9	88	theme	polymerization	1265:1278	arg1	[DP					1280:1282	polymerization [DP	1265:1282	polymerization [DP	1265:1282	Short-chain GOS and FOS (degrees of polymerization [DP] 2 and 3) were favored growth substrates for strains of B. bifidum and B. longum subsp.
32220841	1	89	theme	common	160:165	arg1	species					148:154	Bifidobacterial species	132:154	Bifidobacterial species	132:154	Bifidobacterial species are common inhabitants of the gut of human infants during the period when milk is a major component of the diet.
32220841	1	89	theme	common	160:165	arg1	inhabitants					167:177	common inhabitants	160:177	common inhabitants of the gut of human infants during the period	160:223	Bifidobacterial species are common inhabitants of the gut of human infants during the period when milk is a major component of the diet.
32220841	11	90	theme	short-	1464:1469	arg1	GOS					1488:1490	both short- and longer-chain GOS	1459:1490	both short- and longer-chain GOS	1459:1490	infantis had the ability to utilize both short- and longer-chain GOS and FOS (DP 2 to 6).
32220841	0	91	theme	Characteristic	98:111	arg1	Species					90:96	Bifidobacterial Species	74:96	Bifidobacterial Species Characteristic of the Infant Gut	74:129	Galacto- and Fructo-oligosaccharides Utilized for Growth by Cocultures of Bifidobacterial Species Characteristic of the Infant Gut.
32220841	19	92	theme	ability	2530:2536	arg1	studies					2515:2521	studies	2515:2521	studies of the ability of various bifidobacterial species to grow together in the presence of these oligosaccharides	2515:2630	However, studies of the ability of various bifidobacterial species to grow together in the presence of these oligosaccharides have not been conducted.
32220841	5	93	theme	milk	701:704	arg1	replacement					679:689	replacement	679:689	replacement of breast milk using formula	679:718	Supplementation of the infant diet or replacement of breast milk using formula feeds is common in Western countries.
32220841	5	93	theme	milk	701:704	arg1	diet					671:674	the infant diet	660:674	the infant diet	660:674	Supplementation of the infant diet or replacement of breast milk using formula feeds is common in Western countries.
32220841	18	94	theme	particular	2404:2413	arg1	additives					2431:2439	particular oligosaccharide additives	2404:2439	particular oligosaccharide additives that are generally considered to support bifidobacterial growth	2404:2503	Modern formula milks often contain particular oligosaccharide additives that are generally considered to support bifidobacterial growth.
32220841	5	95	from	common	729:734	arg1	countries					747:755	Western countries	739:755	Western countries	739:755	Supplementation of the infant diet or replacement of breast milk using formula feeds is common in Western countries.
32220841	10	96	theme	B.	1393:1394	arg1	breve					1396:1400	B. breve	1393:1400	B. breve	1393:1400	longum, whereas both B. breve and B. longum subsp.
32220841	0	97	theme	Gut	127:129	arg1	Characteristic					98:111	Characteristic	98:111	Characteristic	98:111	Galacto- and Fructo-oligosaccharides Utilized for Growth by Cocultures of Bifidobacterial Species Characteristic of the Infant Gut.
32220841	19	98	theme	species	2565:2571	arg1	ability					2530:2536	the ability	2526:2536	the ability of various bifidobacterial species to grow together in the presence of these oligosaccharides	2526:2630	However, studies of the ability of various bifidobacterial species to grow together in the presence of these oligosaccharides have not been conducted.
32220841	15	99	theme	bacteria	1844:1851	arg1	kinds					1835:1839	The kinds	1831:1839	The kinds of bacteria that form the collection of microbes (the microbiota) in the gut of human infants	1831:1933	infantis, indicating that the kinetics of substrate utilization is an important ecological factor in the assemblage of gut communities.IMPORTANCE The kinds of bacteria that form the collection of microbes (the microbiota) in the gut of human infants may influence health and well-being.
32220841	9	100	theme	favored	1299:1305	arg1	substrates					1314:1323	favored growth substrates	1299:1323	favored growth substrates for strains of B. bifidum and B. longum subsp	1299:1369	Short-chain GOS and FOS (degrees of polymerization [DP] 2 and 3) were favored growth substrates for strains of B. bifidum and B. longum subsp.
32220841	15	101	theme	human	1921:1925	arg1	infants					1927:1933	human infants	1921:1933	human infants	1921:1933	infantis, indicating that the kinetics of substrate utilization is an important ecological factor in the assemblage of gut communities.IMPORTANCE The kinds of bacteria that form the collection of microbes (the microbiota) in the gut of human infants may influence health and well-being.
32220841	2	102	theme	B.	444:445	arg1	subsp					454:458	B. longum subsp	444:458	B. longum subsp	444:458	Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum subspecies longum, and B. longum subspecies infantis have been detected frequently in infant feces, but B. longum subsp.
32220841	7	103	with	competition	1042:1052	arg1	species					1081:1087	other bifidobacterial species	1059:1087	other bifidobacterial species	1059:1087	infantis to utilize these potential growth substrates when they are in competition with other bifidobacterial species is unknown.
32220841	17	104	theme	modern	2301:2306	arg1	conditions					2322:2331	modern child-rearing conditions	2301:2331	modern child-rearing conditions	2301:2331	Bifidobacterium longum subspecies infantis is a well-known bacterial species, but under modern child-rearing conditions it may be disadvantaged in the gut.
32220841	2	105	theme	infant	426:431	arg1	feces					433:437	infant feces	426:437	infant feces	426:437	Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium longum subspecies longum, and B. longum subspecies infantis have been detected frequently in infant feces, but B. longum subsp.
32220841	10	106	dep	breve	1396:1400	arg1	longum					1372:1377	longum	1372:1377	longum	1372:1377	longum, whereas both B. breve and B. longum subsp.
32220841	12	107	theme	B.	1565:1566	arg1	subsp					1575:1579	B. longum subsp	1565:1579	B. longum subsp	1565:1579	B. breve was nevertheless numerically dominant over B. longum subsp.
32220841	14	108	theme	slower	1639:1644	arg1	use					1646:1648	the slower use	1635:1648	the slower use of GOS of DP 3 by B. longum subsp	1635:1682	This was probably related to the slower use of GOS of DP 3 by B. longum subsp.
32220841	9	109	theme	subsp	1365:1369	arg1	strains					1329:1335	strains	1329:1335	strains of B. bifidum and B. longum subsp	1329:1369	Short-chain GOS and FOS (degrees of polymerization [DP] 2 and 3) were favored growth substrates for strains of B. bifidum and B. longum subsp.
32220841	10	110	dep	B.	1406:1407	arg1	longum					1409:1414	longum	1409:1414	longum	1409:1414	longum, whereas both B. breve and B. longum subsp.
32220841	15	111	theme	important	1755:1763	arg1	factor					1776:1781	an important ecological factor	1752:1781	an important ecological factor in the assemblage of gut communities.IMPORTANCE The kinds of bacteria that form the collection of microbes (the microbiota) in the gut of human infants may influence health and well-being	1752:1969	infantis, indicating that the kinetics of substrate utilization is an important ecological factor in the assemblage of gut communities.IMPORTANCE The kinds of bacteria that form the collection of microbes (the microbiota) in the gut of human infants may influence health and well-being.
32220841	15	111	theme	important	1755:1763	arg1	kinetics					1715:1722	the kinetics	1711:1722	the kinetics of substrate utilization	1711:1747	infantis, indicating that the kinetics of substrate utilization is an important ecological factor in the assemblage of gut communities.IMPORTANCE The kinds of bacteria that form the collection of microbes (the microbiota) in the gut of human infants may influence health and well-being.
32220841	19	112	theme	oligosaccharides	2615:2630	arg1	presence					2597:2604	the presence	2593:2604	the presence of these oligosaccharides	2593:2630	However, studies of the ability of various bifidobacterial species to grow together in the presence of these oligosaccharides have not been conducted.
32220841	18	113	theme	formula	2376:2382	arg1	milks					2384:2388	Modern formula milks	2369:2388	Modern formula milks	2369:2388	Modern formula milks often contain particular oligosaccharide additives that are generally considered to support bifidobacterial growth.
32220841	14	114	theme	B.	1668:1669	arg1	subsp					1678:1682	B. longum subsp	1668:1682	B. longum subsp	1668:1682	This was probably related to the slower use of GOS of DP 3 by B. longum subsp.
32220841	8	115	theme	growth	1117:1122	arg1	utilization					1144:1154	the growth and oligosaccharide utilization	1113:1154	the growth and oligosaccharide utilization of GOS and FOS by bifidobacterial species in pure culture and coculture	1113:1226	We compared the growth and oligosaccharide utilization of GOS and FOS by bifidobacterial species in pure culture and coculture.
32220841	11	116	contain	had	1432:1434	arg2	ability					1440:1446	the ability to utilize both short- and longer-chain GOS and FOS (DP 2 to 6)	1436:1510	the ability to utilize both short- and longer-chain GOS and FOS (DP 2 to 6)	1436:1510	infantis had the ability to utilize both short- and longer-chain GOS and FOS (DP 2 to 6).
32220841	11	116	contain	had	1432:1434	arg1	infantis					1423:1430	infantis	1423:1430	infantis	1423:1430	infantis had the ability to utilize both short- and longer-chain GOS and FOS (DP 2 to 6).
32220841	4	117	theme	breast	597:602	arg1	milk					604:607	breast milk	597:607	breast milk feeding in different countries	597:638	This may be due to the different durations of breast milk feeding in different countries.
32220841	15	118	theme	microbes	1881:1888	arg1	collection					1867:1876	the collection	1863:1876	the collection of microbes (the microbiota)	1863:1905	infantis, indicating that the kinetics of substrate utilization is an important ecological factor in the assemblage of gut communities.IMPORTANCE The kinds of bacteria that form the collection of microbes (the microbiota) in the gut of human infants may influence health and well-being.
32220841	9	119	theme	longum	1358:1363	arg1	subsp					1365:1369	B. bifidum and B. longum subsp	1340:1369	B. bifidum and B. longum subsp	1340:1369	Short-chain GOS and FOS (degrees of polymerization [DP] 2 and 3) were favored growth substrates for strains of B. bifidum and B. longum subsp.
32220841	8	120	theme	oligosaccharide	1128:1142	arg1	utilization					1144:1154	the growth and oligosaccharide utilization	1113:1154	the growth and oligosaccharide utilization of GOS and FOS by bifidobacterial species in pure culture and coculture	1113:1226	We compared the growth and oligosaccharide utilization of GOS and FOS by bifidobacterial species in pure culture and coculture.
32220841	6	121	from	oligosaccharides	899:914	arg1	milks					928:932	ruminant milks	919:932	ruminant milks	919:932	Formula milks often contain galacto- and/or fructo-oligosaccharides (GOS and FOS, respectively) as additives to augment the concentration of oligosaccharides in ruminant milks, but the ability of B. longum subsp.
32220841	3	122	theme	infants	517:523	arg1	gut					510:512	the gut	506:512	the gut of infants in westernized countries	506:548	infantis may be disadvantaged numerically in the gut of infants in westernized countries.
32220841	1	123	theme	major	240:244	arg1	milk					230:233	milk	230:233	milk	230:233	Bifidobacterial species are common inhabitants of the gut of human infants during the period when milk is a major component of the diet.
32220841	1	123	theme	major	240:244	arg1	component					246:254	a major component	238:254	a major component of the diet	238:266	Bifidobacterial species are common inhabitants of the gut of human infants during the period when milk is a major component of the diet.
32565298	0	0	theme	TiO2	93:96	arg1	nanoparticles					98:110	matrix containing TiO2 nanoparticles	75:110	matrix containing TiO2 nanoparticles	75:110	Anionic dye uptake via composite using chitosan-polyacrylamide hydrogel as matrix containing TiO2 nanoparticles; comprehensive adsorption studies.
32565298	0	0	theme	TiO2	93:96	arg1	hydrogel					63:70	chitosan-polyacrylamide hydrogel	39:70	chitosan-polyacrylamide hydrogel	39:70	Anionic dye uptake via composite using chitosan-polyacrylamide hydrogel as matrix containing TiO2 nanoparticles; comprehensive adsorption studies.
32565298	1	1	theme	K-CF	339:342	arg1	dye					344:346	Sirius yellow K-CF dye	325:346	Sirius yellow K-CF dye	325:346	In the present study, TiO2 nanoparticles dispersed in chitosan grafted polyacrylamide matrix (TiO2-PAM-CS) was synthesized using in situ technique, and applied for the uptake of Sirius yellow K-CF dye from aqueous solution.
32565298	3	2	from	time	515:518	arg1	experiments					556:566	batch adsorption experiments	539:566	batch adsorption experiments	539:566	The effect of significant parameters such as pH, dose, time and temperature in batch adsorption experiments were investigated.
32565298	0	3	theme	containing	82:91	arg1	nanoparticles					98:110	matrix containing TiO2 nanoparticles	75:110	matrix containing TiO2 nanoparticles	75:110	Anionic dye uptake via composite using chitosan-polyacrylamide hydrogel as matrix containing TiO2 nanoparticles; comprehensive adsorption studies.
32565298	0	3	theme	containing	82:91	arg1	hydrogel					63:70	chitosan-polyacrylamide hydrogel	39:70	chitosan-polyacrylamide hydrogel	39:70	Anionic dye uptake via composite using chitosan-polyacrylamide hydrogel as matrix containing TiO2 nanoparticles; comprehensive adsorption studies.
32565298	1	4	theme	dye	344:346	arg1	uptake					315:320	the uptake	311:320	the uptake of Sirius yellow K-CF dye from aqueous solution	311:368	In the present study, TiO2 nanoparticles dispersed in chitosan grafted polyacrylamide matrix (TiO2-PAM-CS) was synthesized using in situ technique, and applied for the uptake of Sirius yellow K-CF dye from aqueous solution.
32565298	7	5	theme	Kinetic	937:943	arg1	study					945:949	Kinetic study	937:949	Kinetic study	937:949	Kinetic study also verifies that pseudo second order kinetic model is the predominant model.
32565298	0	6	dep	studies	138:144	arg1	uptake					12:17	Anionic dye uptake	0:17	Anionic dye uptake via composite using chitosan-polyacrylamide hydrogel as matrix containing TiO2 nanoparticles	0:110	Anionic dye uptake via composite using chitosan-polyacrylamide hydrogel as matrix containing TiO2 nanoparticles; comprehensive adsorption studies.
32565298	9	7	theme	HCl	1458:1460	arg1	solution					1462:1469	HCl solution	1458:1469	HCl solution	1458:1469	The reusability test showe that TiO2-PAM-CS composite can be renewed easily with HCl solution as an efficient adsorbent for practical wastewater treatment.
32565298	1	8	theme	in	276:277	arg1	technique					284:292	in situ technique	276:292	in situ technique	276:292	In the present study, TiO2 nanoparticles dispersed in chitosan grafted polyacrylamide matrix (TiO2-PAM-CS) was synthesized using in situ technique, and applied for the uptake of Sirius yellow K-CF dye from aqueous solution.
32565298	8	9	theme	molecules	1133:1141	arg1	molecules					1133:1141	molecules	1133:1141	molecules of anionic dye via hydrogen bond formation (Dye-NH3+)	1133:1195	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	8	9	theme	molecules	1133:1141	arg1	NH3+					1124:1127	NH3+	1124:1127	NH3+	1124:1127	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	8	9	theme	molecules	1133:1141	arg1	interactions					1217:1228	electrostatic interactions	1203:1228	electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model)	1203:1342	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	8	9	theme	molecules	1133:1141	arg1	groups					1060:1065	amin groups	1055:1065	amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model)	1055:1342	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	3	10	from	pH	505:506	arg1	experiments					556:566	batch adsorption experiments	539:566	batch adsorption experiments	539:566	The effect of significant parameters such as pH, dose, time and temperature in batch adsorption experiments were investigated.
32565298	8	11	theme	1.66 kJ/mol	1305:1315	arg1	energy					1295:1300	free energy	1290:1300	free energy of 1.66 kJ/mol calculated from D-R model	1290:1341	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	3	12	theme	batch	539:543	arg1	experiments					556:566	batch adsorption experiments	539:566	batch adsorption experiments	539:566	The effect of significant parameters such as pH, dose, time and temperature in batch adsorption experiments were investigated.
32565298	1	13	theme	aqueous	353:359	arg1	solution					361:368	aqueous solution	353:368	aqueous solution	353:368	In the present study, TiO2 nanoparticles dispersed in chitosan grafted polyacrylamide matrix (TiO2-PAM-CS) was synthesized using in situ technique, and applied for the uptake of Sirius yellow K-CF dye from aqueous solution.
32565298	8	14	theme	PAM-CS	1095:1100	arg1	composite					1103:1111	polyacrylamide-chitosan (PAM-CS) composite	1070:1111	polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model)	1070:1342	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	0	15	theme	adsorption	127:136	arg1	studies					138:144	comprehensive adsorption studies	113:144	comprehensive adsorption studies	113:144	Anionic dye uptake via composite using chitosan-polyacrylamide hydrogel as matrix containing TiO2 nanoparticles; comprehensive adsorption studies.
32565298	4	16	theme	dye	682:684	arg1	removal					686:692	2 with 96.81% dye removal	668:692	2 with 96.81% dye removal	668:692	The adsorption process was pH dependent and the optimum value of pH was obtained 2 with 96.81% dye removal at 40 °C.
32565298	3	17	from	dose	509:512	arg1	experiments					556:566	batch adsorption experiments	539:566	batch adsorption experiments	539:566	The effect of significant parameters such as pH, dose, time and temperature in batch adsorption experiments were investigated.
32565298	8	18	from	available	1243:1251	arg1	composite					1263:1271	composite	1263:1271	composite	1263:1271	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	8	18	from	available	1243:1251	arg1	dye					1285:1287	PAM-CS composite and anionic dye	1256:1287	dye	1285:1287	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	0	19	theme	comprehensive	113:125	arg1	studies					138:144	comprehensive adsorption studies	113:144	comprehensive adsorption studies	113:144	Anionic dye uptake via composite using chitosan-polyacrylamide hydrogel as matrix containing TiO2 nanoparticles; comprehensive adsorption studies.
32565298	6	20	theme	thermodynamic	847:859	arg1	studies					861:867	The Dubinin-Radushkevich (D-R) isotherm and thermodynamic studies	803:867	The Dubinin-Radushkevich (D-R) isotherm and thermodynamic studies	803:867	The Dubinin-Radushkevich (D-R) isotherm and thermodynamic studies prove that the adsorption is physical, endothermic and spontaneous.
32565298	5	21	theme	qm	780:781	arg1	value					783:787	qm value	780:787	qm value of 1000 mg/g	780:800	The equilibrium data were compatible well with the Langmuir isotherm having qm value of 1000 mg/g.
32565298	8	22	theme	anionic	1146:1152	arg1	dye					1154:1156	anionic dye	1146:1156	anionic dye via hydrogen bond formation (Dye-NH3+)	1146:1195	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	0	23	theme	dye	8:10	arg1	uptake					12:17	Anionic dye uptake	0:17	Anionic dye uptake via composite using chitosan-polyacrylamide hydrogel as matrix containing TiO2 nanoparticles	0:110	Anionic dye uptake via composite using chitosan-polyacrylamide hydrogel as matrix containing TiO2 nanoparticles; comprehensive adsorption studies.
32565298	3	24	theme	adsorption	545:554	arg1	experiments					556:566	batch adsorption experiments	539:566	batch adsorption experiments	539:566	The effect of significant parameters such as pH, dose, time and temperature in batch adsorption experiments were investigated.
32565298	8	25	theme	interactions	1217:1228	arg1	molecules					1133:1141	molecules	1133:1141	molecules of anionic dye via hydrogen bond formation (Dye-NH3+)	1133:1195	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	8	25	theme	interactions	1217:1228	arg1	NH3+					1124:1127	NH3+	1124:1127	NH3+	1124:1127	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	8	25	theme	interactions	1217:1228	arg1	interactions					1217:1228	electrostatic interactions	1203:1228	electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model)	1203:1342	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	8	25	theme	interactions	1217:1228	arg1	groups					1060:1065	amin groups	1055:1065	amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model)	1055:1342	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	0	26	theme	Anionic	0:6	arg1	uptake					12:17	Anionic dye uptake	0:17	Anionic dye uptake via composite using chitosan-polyacrylamide hydrogel as matrix containing TiO2 nanoparticles	0:110	Anionic dye uptake via composite using chitosan-polyacrylamide hydrogel as matrix containing TiO2 nanoparticles; comprehensive adsorption studies.
32565298	8	27	dep	in	1113:1114	arg1	from					1116:1119	from	1116:1119	from	1116:1119	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	8	28	theme	NH3+	1124:1127	arg1	molecules					1133:1141	molecules	1133:1141	molecules of anionic dye via hydrogen bond formation (Dye-NH3+)	1133:1195	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	8	28	theme	NH3+	1124:1127	arg1	NH3+					1124:1127	NH3+	1124:1127	NH3+	1124:1127	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	8	28	theme	NH3+	1124:1127	arg1	interactions					1217:1228	electrostatic interactions	1203:1228	electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model)	1203:1342	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	8	28	theme	NH3+	1124:1127	arg1	groups					1060:1065	amin groups	1055:1065	amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model)	1055:1342	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	4	29	dep	removal	686:692	arg1	%					680:680	%	680:680	%	680:680	The adsorption process was pH dependent and the optimum value of pH was obtained 2 with 96.81% dye removal at 40 °C.
32565298	4	30	dep	%	680:680	arg1	96.81					675:679	96.81	675:679	96.81	675:679	The adsorption process was pH dependent and the optimum value of pH was obtained 2 with 96.81% dye removal at 40 °C.
32565298	1	31	theme	chitosan	201:208	arg1	TiO2-PAM-CS					241:251	TiO2-PAM-CS	241:251	TiO2-PAM-CS	241:251	In the present study, TiO2 nanoparticles dispersed in chitosan grafted polyacrylamide matrix (TiO2-PAM-CS) was synthesized using in situ technique, and applied for the uptake of Sirius yellow K-CF dye from aqueous solution.
32565298	1	31	theme	chitosan	201:208	arg1	matrix					233:238	chitosan grafted polyacrylamide matrix	201:238	chitosan grafted polyacrylamide matrix (TiO2-PAM-CS)	201:252	In the present study, TiO2 nanoparticles dispersed in chitosan grafted polyacrylamide matrix (TiO2-PAM-CS) was synthesized using in situ technique, and applied for the uptake of Sirius yellow K-CF dye from aqueous solution.
32565298	8	32	theme	bond	1171:1174	arg1	Dye-NH3+					1187:1194	Dye-NH3+	1187:1194	Dye-NH3+	1187:1194	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	8	32	theme	bond	1171:1174	arg1	formation					1176:1184	hydrogen bond formation	1162:1184	hydrogen bond formation (Dye-NH3+)	1162:1195	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	8	33	from	composite	1263:1271	arg1	available					1243:1251	available	1243:1251	available	1243:1251	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	8	34	from	dye	1285:1287	arg1	available					1243:1251	available	1243:1251	available	1243:1251	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	7	35	theme	pseudo	970:975	arg1	model					1023:1027	the predominant model	1007:1027	the predominant model	1007:1027	Kinetic study also verifies that pseudo second order kinetic model is the predominant model.
32565298	7	35	theme	pseudo	970:975	arg1	model					998:1002	pseudo second order kinetic model	970:1002	pseudo second order kinetic model	970:1002	Kinetic study also verifies that pseudo second order kinetic model is the predominant model.
32565298	1	36	theme	grafted	210:216	arg1	TiO2-PAM-CS					241:251	TiO2-PAM-CS	241:251	TiO2-PAM-CS	241:251	In the present study, TiO2 nanoparticles dispersed in chitosan grafted polyacrylamide matrix (TiO2-PAM-CS) was synthesized using in situ technique, and applied for the uptake of Sirius yellow K-CF dye from aqueous solution.
32565298	1	36	theme	grafted	210:216	arg1	matrix					233:238	chitosan grafted polyacrylamide matrix	201:238	chitosan grafted polyacrylamide matrix (TiO2-PAM-CS)	201:252	In the present study, TiO2 nanoparticles dispersed in chitosan grafted polyacrylamide matrix (TiO2-PAM-CS) was synthesized using in situ technique, and applied for the uptake of Sirius yellow K-CF dye from aqueous solution.
32565298	1	37	from	solution	361:368	arg1	uptake					315:320	the uptake	311:320	the uptake of Sirius yellow K-CF dye from aqueous solution	311:368	In the present study, TiO2 nanoparticles dispersed in chitosan grafted polyacrylamide matrix (TiO2-PAM-CS) was synthesized using in situ technique, and applied for the uptake of Sirius yellow K-CF dye from aqueous solution.
32565298	8	38	theme	amin	1055:1058	arg1	groups					1060:1065	amin groups	1055:1065	amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model)	1055:1342	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	8	38	theme	amin	1055:1058	arg1	composite					1103:1111	polyacrylamide-chitosan (PAM-CS) composite	1070:1111	polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model)	1070:1342	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	6	39	theme	isotherm	834:841	arg1	studies					861:867	The Dubinin-Radushkevich (D-R) isotherm and thermodynamic studies	803:867	The Dubinin-Radushkevich (D-R) isotherm and thermodynamic studies	803:867	The Dubinin-Radushkevich (D-R) isotherm and thermodynamic studies prove that the adsorption is physical, endothermic and spontaneous.
32565298	1	40	theme	TiO2	169:172	arg1	nanoparticles					174:186	TiO2 nanoparticles	169:186	TiO2 nanoparticles dispersed in chitosan grafted polyacrylamide matrix (TiO2-PAM-CS)	169:252	In the present study, TiO2 nanoparticles dispersed in chitosan grafted polyacrylamide matrix (TiO2-PAM-CS) was synthesized using in situ technique, and applied for the uptake of Sirius yellow K-CF dye from aqueous solution.
32565298	9	41	theme	practical	1501:1509	arg1	treatment					1522:1530	practical wastewater treatment	1501:1530	practical wastewater treatment	1501:1530	The reusability test showe that TiO2-PAM-CS composite can be renewed easily with HCl solution as an efficient adsorbent for practical wastewater treatment.
32565298	1	42	theme	polyacrylamide	218:231	arg1	TiO2-PAM-CS					241:251	TiO2-PAM-CS	241:251	TiO2-PAM-CS	241:251	In the present study, TiO2 nanoparticles dispersed in chitosan grafted polyacrylamide matrix (TiO2-PAM-CS) was synthesized using in situ technique, and applied for the uptake of Sirius yellow K-CF dye from aqueous solution.
32565298	1	42	theme	polyacrylamide	218:231	arg1	matrix					233:238	chitosan grafted polyacrylamide matrix	201:238	chitosan grafted polyacrylamide matrix (TiO2-PAM-CS)	201:252	In the present study, TiO2 nanoparticles dispersed in chitosan grafted polyacrylamide matrix (TiO2-PAM-CS) was synthesized using in situ technique, and applied for the uptake of Sirius yellow K-CF dye from aqueous solution.
32565298	8	43	theme	free	1290:1293	arg1	energy					1295:1300	free energy	1290:1300	free energy of 1.66 kJ/mol calculated from D-R model	1290:1341	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	8	44	theme	polyacrylamide-chitosan	1070:1092	arg1	composite					1103:1111	polyacrylamide-chitosan (PAM-CS) composite	1070:1111	polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model)	1070:1342	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	8	45	theme	adsorption	1355:1364	arg1	mechanism					1366:1374	the adsorption mechanism	1351:1374	the adsorption mechanism	1351:1374	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	2	46	theme	synthesized	375:385	arg1	nano-composite					387:400	The synthesized nano-composite	371:400	The synthesized nano-composite	371:400	The synthesized nano-composite was characterized by FE-SEM, TEM, XRD and FT-IR analysis.
32565298	4	47	theme	optimum	635:641	arg1	value					643:647	the optimum value	631:647	the optimum value of pH	631:653	The adsorption process was pH dependent and the optimum value of pH was obtained 2 with 96.81% dye removal at 40 °C.
32565298	9	48	theme	reusability	1381:1391	arg1	test					1393:1396	The reusability test	1377:1396	The reusability test	1377:1396	The reusability test showe that TiO2-PAM-CS composite can be renewed easily with HCl solution as an efficient adsorbent for practical wastewater treatment.
32565298	6	49	theme	D-R	829:831	arg1	isotherm					834:841	The Dubinin-Radushkevich (D-R) isotherm	803:841	The Dubinin-Radushkevich (D-R) isotherm	803:841	The Dubinin-Radushkevich (D-R) isotherm and thermodynamic studies prove that the adsorption is physical, endothermic and spontaneous.
32565298	7	50	theme	kinetic	990:996	arg1	model					1023:1027	the predominant model	1007:1027	the predominant model	1007:1027	Kinetic study also verifies that pseudo second order kinetic model is the predominant model.
32565298	7	50	theme	kinetic	990:996	arg1	model					998:1002	pseudo second order kinetic model	970:1002	pseudo second order kinetic model	970:1002	Kinetic study also verifies that pseudo second order kinetic model is the predominant model.
32565298	5	51	theme	equilibrium	708:718	arg1	compatible					730:739	compatible	730:739	compatible	730:739	The equilibrium data were compatible well with the Langmuir isotherm having qm value of 1000 mg/g.
32565298	5	51	theme	equilibrium	708:718	arg1	data					720:723	The equilibrium data	704:723	The equilibrium data	704:723	The equilibrium data were compatible well with the Langmuir isotherm having qm value of 1000 mg/g.
32565298	3	52	from	temperature	524:534	arg1	experiments					556:566	batch adsorption experiments	539:566	batch adsorption experiments	539:566	The effect of significant parameters such as pH, dose, time and temperature in batch adsorption experiments were investigated.
32565298	7	53	theme	predominant	1011:1021	arg1	model					1023:1027	the predominant model	1007:1027	the predominant model	1007:1027	Kinetic study also verifies that pseudo second order kinetic model is the predominant model.
32565298	7	53	theme	predominant	1011:1021	arg1	model					998:1002	pseudo second order kinetic model	970:1002	pseudo second order kinetic model	970:1002	Kinetic study also verifies that pseudo second order kinetic model is the predominant model.
32565298	5	54	contain	having	773:778	arg1	isotherm					764:771	the Langmuir isotherm	751:771	the Langmuir isotherm having qm value of 1000 mg/g	751:800	The equilibrium data were compatible well with the Langmuir isotherm having qm value of 1000 mg/g.
32565298	5	54	contain	having	773:778	arg2	value					783:787	qm value	780:787	qm value of 1000 mg/g	780:800	The equilibrium data were compatible well with the Langmuir isotherm having qm value of 1000 mg/g.
32565298	8	55	theme	electrostatic	1203:1215	arg1	interactions					1217:1228	electrostatic interactions	1203:1228	electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model)	1203:1342	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	8	56	theme	D-R	1333:1335	arg1	model					1337:1341	D-R model	1333:1341	D-R model	1333:1341	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	3	57	theme	parameters	486:495	arg1	effect					464:469	The effect	460:469	The effect of significant parameters such as pH, dose, time and temperature in batch adsorption experiments	460:566	The effect of significant parameters such as pH, dose, time and temperature in batch adsorption experiments were investigated.
32565298	0	58	theme	chitosan-polyacrylamide	39:61	arg1	nanoparticles					98:110	matrix containing TiO2 nanoparticles	75:110	matrix containing TiO2 nanoparticles	75:110	Anionic dye uptake via composite using chitosan-polyacrylamide hydrogel as matrix containing TiO2 nanoparticles; comprehensive adsorption studies.
32565298	0	58	theme	chitosan-polyacrylamide	39:61	arg1	hydrogel					63:70	chitosan-polyacrylamide hydrogel	39:70	chitosan-polyacrylamide hydrogel	39:70	Anionic dye uptake via composite using chitosan-polyacrylamide hydrogel as matrix containing TiO2 nanoparticles; comprehensive adsorption studies.
32565298	6	59	theme	Dubinin-Radushkevich	807:826	arg1	isotherm					834:841	The Dubinin-Radushkevich (D-R) isotherm	803:841	The Dubinin-Radushkevich (D-R) isotherm	803:841	The Dubinin-Radushkevich (D-R) isotherm and thermodynamic studies prove that the adsorption is physical, endothermic and spontaneous.
32565298	7	60	theme	second	977:982	arg1	model					1023:1027	the predominant model	1007:1027	the predominant model	1007:1027	Kinetic study also verifies that pseudo second order kinetic model is the predominant model.
32565298	7	60	theme	second	977:982	arg1	model					998:1002	pseudo second order kinetic model	970:1002	pseudo second order kinetic model	970:1002	Kinetic study also verifies that pseudo second order kinetic model is the predominant model.
32565298	4	61	theme	pH	652:653	arg1	value					643:647	the optimum value	631:647	the optimum value of pH	631:653	The adsorption process was pH dependent and the optimum value of pH was obtained 2 with 96.81% dye removal at 40 °C.
32565298	1	62	theme	Sirius	325:330	arg1	dye					344:346	Sirius yellow K-CF dye	325:346	Sirius yellow K-CF dye	325:346	In the present study, TiO2 nanoparticles dispersed in chitosan grafted polyacrylamide matrix (TiO2-PAM-CS) was synthesized using in situ technique, and applied for the uptake of Sirius yellow K-CF dye from aqueous solution.
32565298	8	63	theme	composite	1103:1111	arg1	groups					1060:1065	amin groups	1055:1065	amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model)	1055:1342	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	8	63	theme	composite	1103:1111	arg1	composite					1103:1111	polyacrylamide-chitosan (PAM-CS) composite	1070:1111	polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model)	1070:1342	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	5	64	with	compatible	730:739	arg1	isotherm					764:771	the Langmuir isotherm	751:771	the Langmuir isotherm having qm value of 1000 mg/g	751:800	The equilibrium data were compatible well with the Langmuir isotherm having qm value of 1000 mg/g.
32565298	7	65	theme	order	984:988	arg1	model					1023:1027	the predominant model	1007:1027	the predominant model	1007:1027	Kinetic study also verifies that pseudo second order kinetic model is the predominant model.
32565298	7	65	theme	order	984:988	arg1	model					998:1002	pseudo second order kinetic model	970:1002	pseudo second order kinetic model	970:1002	Kinetic study also verifies that pseudo second order kinetic model is the predominant model.
32565298	8	66	theme	anionic	1277:1283	arg1	dye					1285:1287	PAM-CS composite and anionic dye	1256:1287	dye	1285:1287	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	8	67	theme	available	1243:1251	arg1	Ti+4					1238:1241	Ti+4	1238:1241	Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model)	1238:1342	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	9	68	theme	wastewater	1511:1520	arg1	treatment					1522:1530	practical wastewater treatment	1501:1530	practical wastewater treatment	1501:1530	The reusability test showe that TiO2-PAM-CS composite can be renewed easily with HCl solution as an efficient adsorbent for practical wastewater treatment.
32565298	8	69	theme	dye	1154:1156	arg1	molecules					1133:1141	molecules	1133:1141	molecules of anionic dye via hydrogen bond formation (Dye-NH3+)	1133:1195	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	8	69	theme	dye	1154:1156	arg1	NH3+					1124:1127	NH3+	1124:1127	NH3+	1124:1127	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	8	69	theme	dye	1154:1156	arg1	interactions					1217:1228	electrostatic interactions	1203:1228	electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model)	1203:1342	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	3	70	theme	significant	474:484	arg1	parameters					486:495	significant parameters	474:495	significant parameters such as pH, dose, time and temperature in batch adsorption experiments	474:566	The effect of significant parameters such as pH, dose, time and temperature in batch adsorption experiments were investigated.
32565298	3	70	theme	significant	474:484	arg1	pH					505:506	pH	505:506	pH	505:506	The effect of significant parameters such as pH, dose, time and temperature in batch adsorption experiments were investigated.
32565298	3	70	theme	significant	474:484	arg1	temperature					524:534	temperature	524:534	temperature	524:534	The effect of significant parameters such as pH, dose, time and temperature in batch adsorption experiments were investigated.
32565298	3	70	theme	significant	474:484	arg1	time					515:518	time	515:518	time	515:518	The effect of significant parameters such as pH, dose, time and temperature in batch adsorption experiments were investigated.
32565298	3	70	theme	significant	474:484	arg1	dose					509:512	dose	509:512	dose	509:512	The effect of significant parameters such as pH, dose, time and temperature in batch adsorption experiments were investigated.
32565298	4	71	theme	adsorption	591:600	arg1	process					602:608	The adsorption process	587:608	The adsorption process	587:608	The adsorption process was pH dependent and the optimum value of pH was obtained 2 with 96.81% dye removal at 40 °C.
32565298	4	71	theme	adsorption	591:600	arg1	dependent					617:625	dependent	617:625	dependent	617:625	The adsorption process was pH dependent and the optimum value of pH was obtained 2 with 96.81% dye removal at 40 °C.
32565298	0	72	theme	matrix	75:80	arg1	nanoparticles					98:110	matrix containing TiO2 nanoparticles	75:110	matrix containing TiO2 nanoparticles	75:110	Anionic dye uptake via composite using chitosan-polyacrylamide hydrogel as matrix containing TiO2 nanoparticles; comprehensive adsorption studies.
32565298	0	72	theme	matrix	75:80	arg1	hydrogel					63:70	chitosan-polyacrylamide hydrogel	39:70	chitosan-polyacrylamide hydrogel	39:70	Anionic dye uptake via composite using chitosan-polyacrylamide hydrogel as matrix containing TiO2 nanoparticles; comprehensive adsorption studies.
32565298	1	73	dep	in	276:277	arg1	situ					279:282	situ	279:282	situ	279:282	In the present study, TiO2 nanoparticles dispersed in chitosan grafted polyacrylamide matrix (TiO2-PAM-CS) was synthesized using in situ technique, and applied for the uptake of Sirius yellow K-CF dye from aqueous solution.
32565298	8	74	dep	composite	1263:1271	arg1	energy					1295:1300	free energy	1290:1300	free energy of 1.66 kJ/mol calculated from D-R model	1290:1341	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	5	75	theme	1000 mg/g	792:800	arg1	value					783:787	qm value	780:787	qm value of 1000 mg/g	780:800	The equilibrium data were compatible well with the Langmuir isotherm having qm value of 1000 mg/g.
32565298	1	76	theme	present	154:160	arg1	study					162:166	the present study	150:166	the present study	150:166	In the present study, TiO2 nanoparticles dispersed in chitosan grafted polyacrylamide matrix (TiO2-PAM-CS) was synthesized using in situ technique, and applied for the uptake of Sirius yellow K-CF dye from aqueous solution.
32565298	9	77	theme	TiO2-PAM-CS	1409:1419	arg1	composite					1421:1429	TiO2-PAM-CS composite	1409:1429	TiO2-PAM-CS composite	1409:1429	The reusability test showe that TiO2-PAM-CS composite can be renewed easily with HCl solution as an efficient adsorbent for practical wastewater treatment.
32565298	9	77	theme	TiO2-PAM-CS	1409:1419	arg1	adsorbent					1487:1495	adsorbent	1487:1495	adsorbent	1487:1495	The reusability test showe that TiO2-PAM-CS composite can be renewed easily with HCl solution as an efficient adsorbent for practical wastewater treatment.
32565298	5	78	theme	Langmuir	755:762	arg1	isotherm					764:771	the Langmuir isotherm	751:771	the Langmuir isotherm having qm value of 1000 mg/g	751:800	The equilibrium data were compatible well with the Langmuir isotherm having qm value of 1000 mg/g.
32565298	1	79	theme	yellow	332:337	arg1	dye					344:346	Sirius yellow K-CF dye	325:346	Sirius yellow K-CF dye	325:346	In the present study, TiO2 nanoparticles dispersed in chitosan grafted polyacrylamide matrix (TiO2-PAM-CS) was synthesized using in situ technique, and applied for the uptake of Sirius yellow K-CF dye from aqueous solution.
32565298	8	80	theme	hydrogen	1162:1169	arg1	Dye-NH3+					1187:1194	Dye-NH3+	1187:1194	Dye-NH3+	1187:1194	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	8	80	theme	hydrogen	1162:1169	arg1	formation					1176:1184	hydrogen bond formation	1162:1184	hydrogen bond formation (Dye-NH3+)	1162:1195	The interactions between amin groups of polyacrylamide-chitosan (PAM-CS) composite in from of NH3+ and molecules of anionic dye via hydrogen bond formation (Dye-NH3+), also electrostatic interactions between Ti+4 available in PAM-CS composite and anionic dye (free energy of 1.66 kJ/mol calculated from D-R model) govern the adsorption mechanism.
32565298	2	81	theme	FT-IR	444:448	arg1	analysis					450:457	FT-IR analysis	444:457	FT-IR analysis	444:457	The synthesized nano-composite was characterized by FE-SEM, TEM, XRD and FT-IR analysis.
33248610	11	0	theme	P	1747:1747	arg1	interactions					1733:1744	their interactions	1727:1744	their interactions (P > 0.05)	1727:1755	Antibody titer against Newcastle disease (ND) was not affected by probiotics or prebiotics or their interactions (P > 0.05).
33248610	11	0	theme	P	1747:1747	arg1	>					1749:1749	P > 0.05	1747:1754	P > 0.05	1747:1754	Antibody titer against Newcastle disease (ND) was not affected by probiotics or prebiotics or their interactions (P > 0.05).
33248610	12	1	theme	prebiotics	1804:1813	arg1	supplementation					1785:1799	supplementation	1785:1799	supplementation of prebiotics or probiotics	1785:1827	It could be concluded that supplementation of prebiotics or probiotics can improve the growth performance of broilers.
33248610	8	2	theme	=	1114:1114	arg1	P					1112:1112	P = 0.01	1112:1119	P = 0.01	1112:1119	Dietary supplementation of MOS improved weight gain (P = 0.01) and feed conversion ratio (FCR) (P = 0.03) during the overall period, but during starter and finisher periods, weight gain and FCR were not affected by prebiotics.
33248610	8	2	theme	=	1114:1114	arg1	gain					1106:1109	weight gain	1099:1109	weight gain (P = 0.01)	1099:1120	Dietary supplementation of MOS improved weight gain (P = 0.01) and feed conversion ratio (FCR) (P = 0.03) during the overall period, but during starter and finisher periods, weight gain and FCR were not affected by prebiotics.
33248610	3	3	theme	broiler	436:442	arg1	0-21 d					453:458	Nine broiler starter (0-21 d) and finisher (21-35 d) diets	431:488	0-21 d	453:458	Nine broiler starter (0-21 d) and finisher (21-35 d) diets were formulated by using 3 levels of probiotics (0, 1, and 2 g/kg of feed) and 3 levels of MOS (0, 1, and 1.5 g/kg of feed) and were randomly allotted to 9 groups.
33248610	2	4	theme	factorial	408:416	arg1	arrangement					418:428	a 3 × 3 factorial arrangement	400:428	a 3 × 3 factorial arrangement	400:428	A total number of 360-day-old Ross broiler chicks were randomly divided into 9 groups in a 3 × 3 factorial arrangement.
33248610	0	5	from	effect	8:13	arg1	carcass					68:74	carcass	68:74	carcass	68:74	Dietary effect of probiotics and prebiotics on broiler performance, carcass, and immunity.
33248610	0	5	from	effect	8:13	arg1	immunity					81:88	immunity	81:88	immunity	81:88	Dietary effect of probiotics and prebiotics on broiler performance, carcass, and immunity.
33248610	0	5	from	effect	8:13	arg1	performance					55:65	broiler performance	47:65	broiler performance	47:65	Dietary effect of probiotics and prebiotics on broiler performance, carcass, and immunity.
33248610	10	6	theme	Antibody	1458:1465	arg1	titer					1467:1471	Antibody titer	1458:1471	Antibody titer for infectious bursal disease (IBD)	1458:1507	Antibody titer for infectious bursal disease (IBD) was improved (P = 0.026) by the interaction effect between probiotics and prebiotics, when compared with the control group.
33248610	3	7	theme	starter	444:450	arg1	0-21 d					453:458	Nine broiler starter (0-21 d) and finisher (21-35 d) diets	431:488	0-21 d	453:458	Nine broiler starter (0-21 d) and finisher (21-35 d) diets were formulated by using 3 levels of probiotics (0, 1, and 2 g/kg of feed) and 3 levels of MOS (0, 1, and 1.5 g/kg of feed) and were randomly allotted to 9 groups.
33248610	8	8	theme	weight	1233:1238	arg1	gain					1240:1243	weight gain	1233:1243	weight gain	1233:1243	Dietary supplementation of MOS improved weight gain (P = 0.01) and feed conversion ratio (FCR) (P = 0.03) during the overall period, but during starter and finisher periods, weight gain and FCR were not affected by prebiotics.
33248610	2	9	from	groups	390:395	arg1	arrangement					418:428	a 3 × 3 factorial arrangement	400:428	a 3 × 3 factorial arrangement	400:428	A total number of 360-day-old Ross broiler chicks were randomly divided into 9 groups in a 3 × 3 factorial arrangement.
33248610	11	10	theme	Newcastle	1656:1664	arg1	ND					1675:1676	ND	1675:1676	ND	1675:1676	Antibody titer against Newcastle disease (ND) was not affected by probiotics or prebiotics or their interactions (P > 0.05).
33248610	11	10	theme	Newcastle	1656:1664	arg1	disease					1666:1672	Newcastle disease	1656:1672	Newcastle disease (ND)	1656:1677	Antibody titer against Newcastle disease (ND) was not affected by probiotics or prebiotics or their interactions (P > 0.05).
33248610	1	11	from	effects	139:145	arg1	carcasses					268:276	carcasses	268:276	carcasses	268:276	This experiment was carried out to evaluate the effects of dietary addition of probiotics (Protexin) and prebiotics (active MOS, mannan oligosaccharides) on growth performance, carcasses, and antibody titer in broilers.
33248610	1	11	from	effects	139:145	arg1	performance					255:265	growth performance	248:265	growth performance	248:265	This experiment was carried out to evaluate the effects of dietary addition of probiotics (Protexin) and prebiotics (active MOS, mannan oligosaccharides) on growth performance, carcasses, and antibody titer in broilers.
33248610	1	11	from	effects	139:145	arg1	titer					292:296	antibody titer	283:296	antibody titer	283:296	This experiment was carried out to evaluate the effects of dietary addition of probiotics (Protexin) and prebiotics (active MOS, mannan oligosaccharides) on growth performance, carcasses, and antibody titer in broilers.
33248610	3	12	theme	finisher	465:472	arg1	diets					484:488	Nine broiler starter (0-21 d) and finisher (21-35 d) diets	431:488	diets	484:488	Nine broiler starter (0-21 d) and finisher (21-35 d) diets were formulated by using 3 levels of probiotics (0, 1, and 2 g/kg of feed) and 3 levels of MOS (0, 1, and 1.5 g/kg of feed) and were randomly allotted to 9 groups.
33248610	10	13	theme	control	1618:1624	arg1	group					1626:1630	the control group	1614:1630	the control group	1614:1630	Antibody titer for infectious bursal disease (IBD) was improved (P = 0.026) by the interaction effect between probiotics and prebiotics, when compared with the control group.
33248610	1	14	theme	mannan	220:225	arg1	MOS					215:217	active MOS	208:217	active MOS	208:217	This experiment was carried out to evaluate the effects of dietary addition of probiotics (Protexin) and prebiotics (active MOS, mannan oligosaccharides) on growth performance, carcasses, and antibody titer in broilers.
33248610	1	14	theme	mannan	220:225	arg1	oligosaccharides					227:242	mannan oligosaccharides	220:242	mannan oligosaccharides	220:242	This experiment was carried out to evaluate the effects of dietary addition of probiotics (Protexin) and prebiotics (active MOS, mannan oligosaccharides) on growth performance, carcasses, and antibody titer in broilers.
33248610	7	15	theme	starter	955:961	arg1	phases					976:981	the starter and finisher phases	951:981	phases	976:981	While, during the starter and finisher phases, weight gain was increased by probiotics (P = 0.028 or 0.04, respectively).
33248610	1	16	from	carcasses	268:276	arg1	broilers					301:308	broilers	301:308	broilers	301:308	This experiment was carried out to evaluate the effects of dietary addition of probiotics (Protexin) and prebiotics (active MOS, mannan oligosaccharides) on growth performance, carcasses, and antibody titer in broilers.
33248610	6	17	theme	finisher	907:914	arg1	phases					929:934	the starter, finisher, and overall phases	894:934	the starter, finisher, and overall phases	894:934	No interaction (P > 0.05) was observed for weight gain in the starter, finisher, and overall phases.
33248610	6	18	from	gain	886:889	arg1	phases					929:934	the starter, finisher, and overall phases	894:934	the starter, finisher, and overall phases	894:934	No interaction (P > 0.05) was observed for weight gain in the starter, finisher, and overall phases.
33248610	13	19	theme	antibiotic-free	1960:1974	arg1	diets					1976:1980	antibiotic-free diets	1960:1980	antibiotic-free diets	1960:1980	It may also be helpful in improving the antibody titer against IBD in broilers fed antibiotic-free diets.
33248610	2	20	theme	360-day-old	329:339	arg1	chicks					354:359	360-day-old Ross broiler chicks	329:359	360-day-old Ross broiler chicks	329:359	A total number of 360-day-old Ross broiler chicks were randomly divided into 9 groups in a 3 × 3 factorial arrangement.
33248610	2	21	theme	broiler	346:352	arg1	chicks					354:359	360-day-old Ross broiler chicks	329:359	360-day-old Ross broiler chicks	329:359	A total number of 360-day-old Ross broiler chicks were randomly divided into 9 groups in a 3 × 3 factorial arrangement.
33248610	1	22	from	performance	255:265	arg1	broilers					301:308	broilers	301:308	broilers	301:308	This experiment was carried out to evaluate the effects of dietary addition of probiotics (Protexin) and prebiotics (active MOS, mannan oligosaccharides) on growth performance, carcasses, and antibody titer in broilers.
33248610	10	23	theme	interaction	1541:1551	arg1	effect					1553:1558	the interaction effect	1537:1558	the interaction effect between probiotics and prebiotics	1537:1592	Antibody titer for infectious bursal disease (IBD) was improved (P = 0.026) by the interaction effect between probiotics and prebiotics, when compared with the control group.
33248610	6	24	theme	weight	879:884	arg1	gain					886:889	weight gain	879:889	weight gain in the starter, finisher, and overall phases	879:934	No interaction (P > 0.05) was observed for weight gain in the starter, finisher, and overall phases.
33248610	2	25	theme	Ross	341:344	arg1	chicks					354:359	360-day-old Ross broiler chicks	329:359	360-day-old Ross broiler chicks	329:359	A total number of 360-day-old Ross broiler chicks were randomly divided into 9 groups in a 3 × 3 factorial arrangement.
33248610	3	26	theme	MOS	581:583	arg1	levels					571:576	3 levels	569:576	3 levels of MOS (0, 1, and 1.5 g/kg of feed)	569:612	Nine broiler starter (0-21 d) and finisher (21-35 d) diets were formulated by using 3 levels of probiotics (0, 1, and 2 g/kg of feed) and 3 levels of MOS (0, 1, and 1.5 g/kg of feed) and were randomly allotted to 9 groups.
33248610	3	26	theme	MOS	581:583	arg1	levels					517:522	3 levels	515:522	3 levels of probiotics (0, 1, and 2 g/kg of feed)	515:563	Nine broiler starter (0-21 d) and finisher (21-35 d) diets were formulated by using 3 levels of probiotics (0, 1, and 2 g/kg of feed) and 3 levels of MOS (0, 1, and 1.5 g/kg of feed) and were randomly allotted to 9 groups.
33248610	1	27	theme	addition	158:165	arg1	effects					139:145	the effects	135:145	the effects of dietary addition of probiotics (Protexin) and prebiotics (active MOS, mannan oligosaccharides) on growth performance, carcasses, and antibody titer in broilers	135:308	This experiment was carried out to evaluate the effects of dietary addition of probiotics (Protexin) and prebiotics (active MOS, mannan oligosaccharides) on growth performance, carcasses, and antibody titer in broilers.
33248610	0	28	theme	Dietary	0:6	arg1	effect					8:13	Dietary effect	0:13	Dietary effect of probiotics and prebiotics on broiler performance, carcass, and immunity	0:88	Dietary effect of probiotics and prebiotics on broiler performance, carcass, and immunity.
33248610	8	29	theme	feed	1126:1129	arg1	FCR					1149:1151	FCR	1149:1151	FCR	1149:1151	Dietary supplementation of MOS improved weight gain (P = 0.01) and feed conversion ratio (FCR) (P = 0.03) during the overall period, but during starter and finisher periods, weight gain and FCR were not affected by prebiotics.
33248610	8	29	theme	feed	1126:1129	arg1	P =					1155:1157	P = 0.03	1155:1162	P = 0.03	1155:1162	Dietary supplementation of MOS improved weight gain (P = 0.01) and feed conversion ratio (FCR) (P = 0.03) during the overall period, but during starter and finisher periods, weight gain and FCR were not affected by prebiotics.
33248610	8	29	theme	feed	1126:1129	arg1	ratio					1142:1146	feed conversion ratio	1126:1146	feed conversion ratio (FCR) (P = 0.03)	1126:1163	Dietary supplementation of MOS improved weight gain (P = 0.01) and feed conversion ratio (FCR) (P = 0.03) during the overall period, but during starter and finisher periods, weight gain and FCR were not affected by prebiotics.
33248610	0	30	theme	probiotics	18:27	arg1	effect					8:13	Dietary effect	0:13	Dietary effect of probiotics and prebiotics on broiler performance, carcass, and immunity	0:88	Dietary effect of probiotics and prebiotics on broiler performance, carcass, and immunity.
33248610	9	31	theme	prebiotics	1372:1381	arg1	effect					1347:1352	no interaction or individual effect	1318:1352	effect	1347:1352	Apart from dressing percentage, no interaction or individual effect of probiotics and prebiotics was observed for carcass, breast, thigh, heart, liver, and gizzard weight.
33248610	4	32	theme	P	731:731	arg1	phases					723:728	all phases	719:728	all phases (P > 0.05)	719:739	Feed intake was not affected by interaction of treatments during all phases (P > 0.05).
33248610	4	32	theme	P	731:731	arg1	>					733:733	P > 0.05	731:738	P > 0.05	731:738	Feed intake was not affected by interaction of treatments during all phases (P > 0.05).
33248610	5	33	theme	=	806:806	arg1	P					804:804	P = 0.0001	804:813	P = 0.0001	804:813	Feed intake was improved due to the main effect of probiotic (P = 0.0001) or MOS (P = 0.005).
33248610	5	33	theme	=	806:806	arg1	probiotic					793:801	probiotic	793:801	probiotic	793:801	Feed intake was improved due to the main effect of probiotic (P = 0.0001) or MOS (P = 0.005).
33248610	3	34	theme	21-35 d	475:481	arg1	diets					484:488	Nine broiler starter (0-21 d) and finisher (21-35 d) diets	431:488	diets	484:488	Nine broiler starter (0-21 d) and finisher (21-35 d) diets were formulated by using 3 levels of probiotics (0, 1, and 2 g/kg of feed) and 3 levels of MOS (0, 1, and 1.5 g/kg of feed) and were randomly allotted to 9 groups.
33248610	8	35	theme	Dietary	1059:1065	arg1	supplementation					1067:1081	Dietary supplementation	1059:1081	Dietary supplementation of MOS	1059:1088	Dietary supplementation of MOS improved weight gain (P = 0.01) and feed conversion ratio (FCR) (P = 0.03) during the overall period, but during starter and finisher periods, weight gain and FCR were not affected by prebiotics.
33248610	1	36	theme	probiotics	170:179	arg1	addition					158:165	dietary addition	150:165	dietary addition of probiotics (Protexin) and prebiotics (active MOS, mannan oligosaccharides)	150:243	This experiment was carried out to evaluate the effects of dietary addition of probiotics (Protexin) and prebiotics (active MOS, mannan oligosaccharides) on growth performance, carcasses, and antibody titer in broilers.
33248610	1	37	theme	growth	248:253	arg1	performance					255:265	growth performance	248:265	growth performance	248:265	This experiment was carried out to evaluate the effects of dietary addition of probiotics (Protexin) and prebiotics (active MOS, mannan oligosaccharides) on growth performance, carcasses, and antibody titer in broilers.
33248610	12	38	theme	growth	1845:1850	arg1	performance					1852:1862	the growth performance	1841:1862	the growth performance of broilers	1841:1874	It could be concluded that supplementation of prebiotics or probiotics can improve the growth performance of broilers.
33248610	12	39	theme	broilers	1867:1874	arg1	performance					1852:1862	the growth performance	1841:1862	the growth performance of broilers	1841:1874	It could be concluded that supplementation of prebiotics or probiotics can improve the growth performance of broilers.
33248610	0	40	theme	prebiotics	33:42	arg1	effect					8:13	Dietary effect	0:13	Dietary effect of probiotics and prebiotics on broiler performance, carcass, and immunity	0:88	Dietary effect of probiotics and prebiotics on broiler performance, carcass, and immunity.
33248610	7	41	theme	weight	984:989	arg1	gain					991:994	weight gain	984:994	weight gain	984:994	While, during the starter and finisher phases, weight gain was increased by probiotics (P = 0.028 or 0.04, respectively).
33248610	5	42	theme	probiotic	793:801	arg1	effect					783:788	the main effect	774:788	the main effect of probiotic (P = 0.0001) or MOS (P = 0.005)	774:833	Feed intake was improved due to the main effect of probiotic (P = 0.0001) or MOS (P = 0.005).
33248610	3	43	dep	probiotics	527:536	arg1	1					542:542	1	542:542	1	542:542	Nine broiler starter (0-21 d) and finisher (21-35 d) diets were formulated by using 3 levels of probiotics (0, 1, and 2 g/kg of feed) and 3 levels of MOS (0, 1, and 1.5 g/kg of feed) and were randomly allotted to 9 groups.
33248610	3	43	dep	probiotics	527:536	arg1	2 g/kg					549:554	2 g/kg	549:554	2 g/kg	549:554	Nine broiler starter (0-21 d) and finisher (21-35 d) diets were formulated by using 3 levels of probiotics (0, 1, and 2 g/kg of feed) and 3 levels of MOS (0, 1, and 1.5 g/kg of feed) and were randomly allotted to 9 groups.
33248610	3	43	dep	probiotics	527:536	arg1	0					539:539	0	539:539	0	539:539	Nine broiler starter (0-21 d) and finisher (21-35 d) diets were formulated by using 3 levels of probiotics (0, 1, and 2 g/kg of feed) and 3 levels of MOS (0, 1, and 1.5 g/kg of feed) and were randomly allotted to 9 groups.
33248610	3	43	dep	probiotics	527:536	arg1	feed					559:562	feed	559:562	feed	559:562	Nine broiler starter (0-21 d) and finisher (21-35 d) diets were formulated by using 3 levels of probiotics (0, 1, and 2 g/kg of feed) and 3 levels of MOS (0, 1, and 1.5 g/kg of feed) and were randomly allotted to 9 groups.
33248610	2	44	theme	×	404:404	arg1	arrangement					418:428	a 3 × 3 factorial arrangement	400:428	a 3 × 3 factorial arrangement	400:428	A total number of 360-day-old Ross broiler chicks were randomly divided into 9 groups in a 3 × 3 factorial arrangement.
33248610	3	45	theme	feed	608:611	arg1	feed					608:611	feed	608:611	feed	608:611	Nine broiler starter (0-21 d) and finisher (21-35 d) diets were formulated by using 3 levels of probiotics (0, 1, and 2 g/kg of feed) and 3 levels of MOS (0, 1, and 1.5 g/kg of feed) and were randomly allotted to 9 groups.
33248610	3	45	theme	feed	608:611	arg1	0					586:586	0	586:586	0	586:586	Nine broiler starter (0-21 d) and finisher (21-35 d) diets were formulated by using 3 levels of probiotics (0, 1, and 2 g/kg of feed) and 3 levels of MOS (0, 1, and 1.5 g/kg of feed) and were randomly allotted to 9 groups.
33248610	5	46	theme	=	826:826	arg1	P					824:824	P = 0.005	824:832	P = 0.005	824:832	Feed intake was improved due to the main effect of probiotic (P = 0.0001) or MOS (P = 0.005).
33248610	5	46	theme	=	826:826	arg1	MOS					819:821	MOS	819:821	MOS (P = 0.005)	819:833	Feed intake was improved due to the main effect of probiotic (P = 0.0001) or MOS (P = 0.005).
33248610	13	47	from	titer	1926:1930	arg1	broilers					1947:1954	broilers	1947:1954	broilers fed antibiotic-free diets	1947:1980	It may also be helpful in improving the antibody titer against IBD in broilers fed antibiotic-free diets.
33248610	2	48	theme	chicks	354:359	arg1	number					319:324	A total number	311:324	A total number of 360-day-old Ross broiler chicks	311:359	A total number of 360-day-old Ross broiler chicks were randomly divided into 9 groups in a 3 × 3 factorial arrangement.
33248610	0	49	theme	broiler	47:53	arg1	performance					55:65	broiler performance	47:65	broiler performance	47:65	Dietary effect of probiotics and prebiotics on broiler performance, carcass, and immunity.
33248610	3	50	dep	MOS	581:583	arg1	feed					608:611	feed	608:611	feed	608:611	Nine broiler starter (0-21 d) and finisher (21-35 d) diets were formulated by using 3 levels of probiotics (0, 1, and 2 g/kg of feed) and 3 levels of MOS (0, 1, and 1.5 g/kg of feed) and were randomly allotted to 9 groups.
33248610	3	50	dep	MOS	581:583	arg1	1					589:589	1	589:589	1	589:589	Nine broiler starter (0-21 d) and finisher (21-35 d) diets were formulated by using 3 levels of probiotics (0, 1, and 2 g/kg of feed) and 3 levels of MOS (0, 1, and 1.5 g/kg of feed) and were randomly allotted to 9 groups.
33248610	3	50	dep	MOS	581:583	arg1	1.5 g/kg					596:603	1.5 g/kg	596:603	1.5 g/kg	596:603	Nine broiler starter (0-21 d) and finisher (21-35 d) diets were formulated by using 3 levels of probiotics (0, 1, and 2 g/kg of feed) and 3 levels of MOS (0, 1, and 1.5 g/kg of feed) and were randomly allotted to 9 groups.
33248610	3	50	dep	MOS	581:583	arg1	0					586:586	0	586:586	0	586:586	Nine broiler starter (0-21 d) and finisher (21-35 d) diets were formulated by using 3 levels of probiotics (0, 1, and 2 g/kg of feed) and 3 levels of MOS (0, 1, and 1.5 g/kg of feed) and were randomly allotted to 9 groups.
33248610	9	51	theme	interaction	1321:1331	arg1	effect					1347:1352	no interaction or individual effect	1318:1352	effect	1347:1352	Apart from dressing percentage, no interaction or individual effect of probiotics and prebiotics was observed for carcass, breast, thigh, heart, liver, and gizzard weight.
33248610	1	52	dep	prebiotics	196:205	arg1	MOS					215:217	active MOS	208:217	active MOS	208:217	This experiment was carried out to evaluate the effects of dietary addition of probiotics (Protexin) and prebiotics (active MOS, mannan oligosaccharides) on growth performance, carcasses, and antibody titer in broilers.
33248610	1	52	dep	prebiotics	196:205	arg1	oligosaccharides					227:242	mannan oligosaccharides	220:242	mannan oligosaccharides	220:242	This experiment was carried out to evaluate the effects of dietary addition of probiotics (Protexin) and prebiotics (active MOS, mannan oligosaccharides) on growth performance, carcasses, and antibody titer in broilers.
33248610	6	53	theme	overall	921:927	arg1	phases					929:934	the starter, finisher, and overall phases	894:934	the starter, finisher, and overall phases	894:934	No interaction (P > 0.05) was observed for weight gain in the starter, finisher, and overall phases.
33248610	7	54	theme	finisher	967:974	arg1	phases					976:981	the starter and finisher phases	951:981	phases	976:981	While, during the starter and finisher phases, weight gain was increased by probiotics (P = 0.028 or 0.04, respectively).
33248610	1	55	from	titer	292:296	arg1	broilers					301:308	broilers	301:308	broilers	301:308	This experiment was carried out to evaluate the effects of dietary addition of probiotics (Protexin) and prebiotics (active MOS, mannan oligosaccharides) on growth performance, carcasses, and antibody titer in broilers.
33248610	8	56	theme	starter	1203:1209	arg1	periods					1224:1230	starter and finisher periods	1203:1230	periods	1224:1230	Dietary supplementation of MOS improved weight gain (P = 0.01) and feed conversion ratio (FCR) (P = 0.03) during the overall period, but during starter and finisher periods, weight gain and FCR were not affected by prebiotics.
33248610	4	57	theme	treatments	701:710	arg1	interaction					686:696	interaction	686:696	interaction of treatments	686:710	Feed intake was not affected by interaction of treatments during all phases (P > 0.05).
33248610	8	58	theme	overall	1176:1182	arg1	period					1184:1189	the overall period	1172:1189	the overall period	1172:1189	Dietary supplementation of MOS improved weight gain (P = 0.01) and feed conversion ratio (FCR) (P = 0.03) during the overall period, but during starter and finisher periods, weight gain and FCR were not affected by prebiotics.
33248610	4	59	theme	Feed	654:657	arg1	intake					659:664	Feed intake	654:664	Feed intake	654:664	Feed intake was not affected by interaction of treatments during all phases (P > 0.05).
33248610	10	60	theme	infectious	1477:1486	arg1	disease					1495:1501	infectious bursal disease	1477:1501	infectious bursal disease (IBD)	1477:1507	Antibody titer for infectious bursal disease (IBD) was improved (P = 0.026) by the interaction effect between probiotics and prebiotics, when compared with the control group.
33248610	10	60	theme	infectious	1477:1486	arg1	IBD					1504:1506	IBD	1504:1506	IBD	1504:1506	Antibody titer for infectious bursal disease (IBD) was improved (P = 0.026) by the interaction effect between probiotics and prebiotics, when compared with the control group.
33248610	10	61	theme	bursal	1488:1493	arg1	disease					1495:1501	infectious bursal disease	1477:1501	infectious bursal disease (IBD)	1477:1507	Antibody titer for infectious bursal disease (IBD) was improved (P = 0.026) by the interaction effect between probiotics and prebiotics, when compared with the control group.
33248610	10	61	theme	bursal	1488:1493	arg1	IBD					1504:1506	IBD	1504:1506	IBD	1504:1506	Antibody titer for infectious bursal disease (IBD) was improved (P = 0.026) by the interaction effect between probiotics and prebiotics, when compared with the control group.
33248610	8	62	theme	weight	1099:1104	arg1	P					1112:1112	P = 0.01	1112:1119	P = 0.01	1112:1119	Dietary supplementation of MOS improved weight gain (P = 0.01) and feed conversion ratio (FCR) (P = 0.03) during the overall period, but during starter and finisher periods, weight gain and FCR were not affected by prebiotics.
33248610	8	62	theme	weight	1099:1104	arg1	gain					1106:1109	weight gain	1099:1109	weight gain (P = 0.01)	1099:1120	Dietary supplementation of MOS improved weight gain (P = 0.01) and feed conversion ratio (FCR) (P = 0.03) during the overall period, but during starter and finisher periods, weight gain and FCR were not affected by prebiotics.
33248610	9	63	theme	individual	1336:1345	arg1	effect					1347:1352	no interaction or individual effect	1318:1352	effect	1347:1352	Apart from dressing percentage, no interaction or individual effect of probiotics and prebiotics was observed for carcass, breast, thigh, heart, liver, and gizzard weight.
33248610	3	64	theme	feed	559:562	arg1	0					539:539	0	539:539	0	539:539	Nine broiler starter (0-21 d) and finisher (21-35 d) diets were formulated by using 3 levels of probiotics (0, 1, and 2 g/kg of feed) and 3 levels of MOS (0, 1, and 1.5 g/kg of feed) and were randomly allotted to 9 groups.
33248610	3	64	theme	feed	559:562	arg1	feed					559:562	feed	559:562	feed	559:562	Nine broiler starter (0-21 d) and finisher (21-35 d) diets were formulated by using 3 levels of probiotics (0, 1, and 2 g/kg of feed) and 3 levels of MOS (0, 1, and 1.5 g/kg of feed) and were randomly allotted to 9 groups.
33248610	3	65	theme	probiotics	527:536	arg1	levels					571:576	3 levels	569:576	3 levels of MOS (0, 1, and 1.5 g/kg of feed)	569:612	Nine broiler starter (0-21 d) and finisher (21-35 d) diets were formulated by using 3 levels of probiotics (0, 1, and 2 g/kg of feed) and 3 levels of MOS (0, 1, and 1.5 g/kg of feed) and were randomly allotted to 9 groups.
33248610	3	65	theme	probiotics	527:536	arg1	levels					517:522	3 levels	515:522	3 levels of probiotics (0, 1, and 2 g/kg of feed)	515:563	Nine broiler starter (0-21 d) and finisher (21-35 d) diets were formulated by using 3 levels of probiotics (0, 1, and 2 g/kg of feed) and 3 levels of MOS (0, 1, and 1.5 g/kg of feed) and were randomly allotted to 9 groups.
33248610	6	66	theme	P	852:852	arg1	interaction					839:849	No interaction	836:849	No interaction (P > 0.05)	836:860	No interaction (P > 0.05) was observed for weight gain in the starter, finisher, and overall phases.
33248610	6	66	theme	P	852:852	arg1	>					854:854	P > 0.05	852:859	P > 0.05	852:859	No interaction (P > 0.05) was observed for weight gain in the starter, finisher, and overall phases.
33248610	5	67	theme	Feed	742:745	arg1	intake					747:752	Feed intake	742:752	Feed intake	742:752	Feed intake was improved due to the main effect of probiotic (P = 0.0001) or MOS (P = 0.005).
33248610	8	68	theme	finisher	1215:1222	arg1	periods					1224:1230	starter and finisher periods	1203:1230	periods	1224:1230	Dietary supplementation of MOS improved weight gain (P = 0.01) and feed conversion ratio (FCR) (P = 0.03) during the overall period, but during starter and finisher periods, weight gain and FCR were not affected by prebiotics.
33248610	11	69	theme	Antibody	1633:1640	arg1	titer					1642:1646	Antibody titer	1633:1646	Antibody titer against Newcastle disease (ND)	1633:1677	Antibody titer against Newcastle disease (ND) was not affected by probiotics or prebiotics or their interactions (P > 0.05).
33248610	8	70	theme	conversion	1131:1140	arg1	FCR					1149:1151	FCR	1149:1151	FCR	1149:1151	Dietary supplementation of MOS improved weight gain (P = 0.01) and feed conversion ratio (FCR) (P = 0.03) during the overall period, but during starter and finisher periods, weight gain and FCR were not affected by prebiotics.
33248610	8	70	theme	conversion	1131:1140	arg1	P =					1155:1157	P = 0.03	1155:1162	P = 0.03	1155:1162	Dietary supplementation of MOS improved weight gain (P = 0.01) and feed conversion ratio (FCR) (P = 0.03) during the overall period, but during starter and finisher periods, weight gain and FCR were not affected by prebiotics.
33248610	8	70	theme	conversion	1131:1140	arg1	ratio					1142:1146	feed conversion ratio	1126:1146	feed conversion ratio (FCR) (P = 0.03)	1126:1163	Dietary supplementation of MOS improved weight gain (P = 0.01) and feed conversion ratio (FCR) (P = 0.03) during the overall period, but during starter and finisher periods, weight gain and FCR were not affected by prebiotics.
33248610	5	71	theme	main	778:781	arg1	effect					783:788	the main effect	774:788	the main effect of probiotic (P = 0.0001) or MOS (P = 0.005)	774:833	Feed intake was improved due to the main effect of probiotic (P = 0.0001) or MOS (P = 0.005).
33248610	13	72	theme	antibody	1917:1924	arg1	titer					1926:1930	the antibody titer	1913:1930	the antibody titer against IBD in broilers fed antibiotic-free diets	1913:1980	It may also be helpful in improving the antibody titer against IBD in broilers fed antibiotic-free diets.
33248610	9	73	theme	gizzard	1442:1448	arg1	weight					1450:1455	gizzard weight	1442:1455	gizzard weight	1442:1455	Apart from dressing percentage, no interaction or individual effect of probiotics and prebiotics was observed for carcass, breast, thigh, heart, liver, and gizzard weight.
33248610	1	74	theme	prebiotics	196:205	arg1	addition					158:165	dietary addition	150:165	dietary addition of probiotics (Protexin) and prebiotics (active MOS, mannan oligosaccharides)	150:243	This experiment was carried out to evaluate the effects of dietary addition of probiotics (Protexin) and prebiotics (active MOS, mannan oligosaccharides) on growth performance, carcasses, and antibody titer in broilers.
33248610	8	75	theme	MOS	1086:1088	arg1	supplementation					1067:1081	Dietary supplementation	1059:1081	Dietary supplementation of MOS	1059:1088	Dietary supplementation of MOS improved weight gain (P = 0.01) and feed conversion ratio (FCR) (P = 0.03) during the overall period, but during starter and finisher periods, weight gain and FCR were not affected by prebiotics.
33248610	5	76	theme	MOS	819:821	arg1	effect					783:788	the main effect	774:788	the main effect of probiotic (P = 0.0001) or MOS (P = 0.005)	774:833	Feed intake was improved due to the main effect of probiotic (P = 0.0001) or MOS (P = 0.005).
33248610	9	77	theme	dressing	1297:1304	arg1	percentage					1306:1315	dressing percentage	1297:1315	dressing percentage	1297:1315	Apart from dressing percentage, no interaction or individual effect of probiotics and prebiotics was observed for carcass, breast, thigh, heart, liver, and gizzard weight.
33248610	1	78	theme	dietary	150:156	arg1	addition					158:165	dietary addition	150:165	dietary addition of probiotics (Protexin) and prebiotics (active MOS, mannan oligosaccharides)	150:243	This experiment was carried out to evaluate the effects of dietary addition of probiotics (Protexin) and prebiotics (active MOS, mannan oligosaccharides) on growth performance, carcasses, and antibody titer in broilers.
33248610	12	79	theme	probiotics	1818:1827	arg1	supplementation					1785:1799	supplementation	1785:1799	supplementation of prebiotics or probiotics	1785:1827	It could be concluded that supplementation of prebiotics or probiotics can improve the growth performance of broilers.
33248610	9	80	theme	probiotics	1357:1366	arg1	effect					1347:1352	no interaction or individual effect	1318:1352	effect	1347:1352	Apart from dressing percentage, no interaction or individual effect of probiotics and prebiotics was observed for carcass, breast, thigh, heart, liver, and gizzard weight.
33248610	1	81	theme	antibody	283:290	arg1	titer					292:296	antibody titer	283:296	antibody titer	283:296	This experiment was carried out to evaluate the effects of dietary addition of probiotics (Protexin) and prebiotics (active MOS, mannan oligosaccharides) on growth performance, carcasses, and antibody titer in broilers.
33248610	6	82	theme	starter	898:904	arg1	phases					929:934	the starter, finisher, and overall phases	894:934	the starter, finisher, and overall phases	894:934	No interaction (P > 0.05) was observed for weight gain in the starter, finisher, and overall phases.
33248610	2	83	theme	total	313:317	arg1	number					319:324	A total number	311:324	A total number of 360-day-old Ross broiler chicks	311:359	A total number of 360-day-old Ross broiler chicks were randomly divided into 9 groups in a 3 × 3 factorial arrangement.
33248610	1	84	theme	active	208:213	arg1	MOS					215:217	active MOS	208:217	active MOS	208:217	This experiment was carried out to evaluate the effects of dietary addition of probiotics (Protexin) and prebiotics (active MOS, mannan oligosaccharides) on growth performance, carcasses, and antibody titer in broilers.
33248610	1	84	theme	active	208:213	arg1	oligosaccharides					227:242	mannan oligosaccharides	220:242	mannan oligosaccharides	220:242	This experiment was carried out to evaluate the effects of dietary addition of probiotics (Protexin) and prebiotics (active MOS, mannan oligosaccharides) on growth performance, carcasses, and antibody titer in broilers.
33309655	0	0	theme	pressure-free	88:100	arg1	design					102:107	a pressure-free design	86:107	a pressure-free design	86:107	Chitosan functionalized AgNPs for efficient removal of Imidacloprid pesticide through a pressure-free design.
33309655	8	1	theme	initial	1151:1157	arg1	concentration					1159:1171	its initial concentration	1147:1171	its initial concentration	1147:1171	Moreover, the amount of removed Imidacloprid was proportional with the amount of its initial concentration indicating the successful removal of Imidacloprid by AgNPs @chitosan membrane even at higher pesticide concentrations.
33309655	9	2	theme	membranes	1359:1367	arg1	use					1336:1338	the promising use	1322:1338	the promising use of AgNPs @chitosan membranes for removal of Imidacloprid pesticide from contaminated water	1322:1429	The obtained results indicate the promising use of AgNPs @chitosan membranes for removal of Imidacloprid pesticide from contaminated water depending on the pressure-free design that lacks external energy support.
33309655	9	3	theme	AgNPs	1343:1347	arg1	membranes					1359:1367	AgNPs @chitosan membranes	1343:1367	AgNPs @chitosan membranes	1343:1367	The obtained results indicate the promising use of AgNPs @chitosan membranes for removal of Imidacloprid pesticide from contaminated water depending on the pressure-free design that lacks external energy support.
33309655	8	4	theme	concentration	1159:1171	arg1	concentration					1159:1171	its initial concentration	1147:1171	its initial concentration	1147:1171	Moreover, the amount of removed Imidacloprid was proportional with the amount of its initial concentration indicating the successful removal of Imidacloprid by AgNPs @chitosan membrane even at higher pesticide concentrations.
33309655	8	4	theme	concentration	1159:1171	arg1	amount					1137:1142	the amount	1133:1142	the amount of its initial concentration indicating the successful removal of Imidacloprid by AgNPs @chitosan membrane even at higher pesticide concentrations	1133:1289	Moreover, the amount of removed Imidacloprid was proportional with the amount of its initial concentration indicating the successful removal of Imidacloprid by AgNPs @chitosan membrane even at higher pesticide concentrations.
33309655	5	5	theme	SEM	706:708	arg1	analysis					723:730	SEM, TEM and PSA analysis	706:730	SEM, TEM and PSA analysis	706:730	SEM, TEM and PSA analysis revealed the biosynthesis of AgNPs in the range of 25-50 nm.
33309655	7	6	theme	40	1002:1003	arg1	Imidacloprid					1016:1027	Imidacloprid	1016:1027	Imidacloprid	1016:1027	Both of chitosan and AgNPs @chitosan membranes succeeded to remove 40 and 85% of Imidacloprid at slightly acidic pH, respectively.
33309655	7	6	theme	40	1002:1003	arg1	%					1011:1011	40 and 85%	1002:1011	%	1011:1011	Both of chitosan and AgNPs @chitosan membranes succeeded to remove 40 and 85% of Imidacloprid at slightly acidic pH, respectively.
33309655	8	7	theme	successful	1188:1197	arg1	removal					1199:1205	the successful removal	1184:1205	the successful removal of Imidacloprid by AgNPs @chitosan membrane	1184:1249	Moreover, the amount of removed Imidacloprid was proportional with the amount of its initial concentration indicating the successful removal of Imidacloprid by AgNPs @chitosan membrane even at higher pesticide concentrations.
33309655	6	8	theme	proper	837:842	arg1	formation					844:852	the proper formation	833:852	the proper formation of chitosan membrane	833:873	However, SEM and FTIR analysis revealed the proper formation of chitosan membrane and its proper functionalization with silver nanoparticles.
33309655	3	9	theme	current	383:389	arg1	study					391:395	The current study	379:395	The current study	379:395	The current study is concerning by removal of Imidacloprid pesticide from water using pressure-free passage through polymeric membrane integrated design.
33309655	4	10	theme	adsorbent	671:679	arg1	chitosan					541:548	chitosan	541:548	chitosan	541:548	Both of chitosan and chitosan functionalized silver nanoparticles (AgNPs @chitosan) membranes were prepared, characterized and applied as adsorbent matrix for Imidacloprid.
33309655	4	10	theme	adsorbent	671:679	arg1	Both					533:536	Both	533:536	Both	533:536	Both of chitosan and chitosan functionalized silver nanoparticles (AgNPs @chitosan) membranes were prepared, characterized and applied as adsorbent matrix for Imidacloprid.
33309655	4	10	theme	adsorbent	671:679	arg1	matrix					681:686	adsorbent matrix	671:686	adsorbent matrix for Imidacloprid	671:703	Both of chitosan and chitosan functionalized silver nanoparticles (AgNPs @chitosan) membranes were prepared, characterized and applied as adsorbent matrix for Imidacloprid.
33309655	4	10	theme	adsorbent	671:679	arg1	chitosan					554:561	chitosan	554:561	chitosan	554:561	Both of chitosan and chitosan functionalized silver nanoparticles (AgNPs @chitosan) membranes were prepared, characterized and applied as adsorbent matrix for Imidacloprid.
33309655	5	11	theme	TEM	711:713	arg1	analysis					723:730	SEM, TEM and PSA analysis	706:730	SEM, TEM and PSA analysis	706:730	SEM, TEM and PSA analysis revealed the biosynthesis of AgNPs in the range of 25-50 nm.
33309655	5	12	from	biosynthesis	745:756	arg1	range					774:778	the range	770:778	the range of 25-50 nm	770:790	SEM, TEM and PSA analysis revealed the biosynthesis of AgNPs in the range of 25-50 nm.
33309655	9	13	theme	@	1349:1349	arg1	membranes					1359:1367	AgNPs @chitosan membranes	1343:1367	AgNPs @chitosan membranes	1343:1367	The obtained results indicate the promising use of AgNPs @chitosan membranes for removal of Imidacloprid pesticide from contaminated water depending on the pressure-free design that lacks external energy support.
33309655	3	14	theme	membrane	505:512	arg1	design					525:530	polymeric membrane integrated design	495:530	polymeric membrane integrated design	495:530	The current study is concerning by removal of Imidacloprid pesticide from water using pressure-free passage through polymeric membrane integrated design.
33309655	4	15	dep	chitosan	554:561	arg1	nanoparticles					585:597	functionalized silver nanoparticles	563:597	functionalized silver nanoparticles (AgNPs @chitosan)	563:615	Both of chitosan and chitosan functionalized silver nanoparticles (AgNPs @chitosan) membranes were prepared, characterized and applied as adsorbent matrix for Imidacloprid.
33309655	4	15	dep	chitosan	554:561	arg1	chitosan					607:614	AgNPs @chitosan	600:614	AgNPs @chitosan	600:614	Both of chitosan and chitosan functionalized silver nanoparticles (AgNPs @chitosan) membranes were prepared, characterized and applied as adsorbent matrix for Imidacloprid.
33309655	8	16	theme	AgNPs	1226:1230	arg1	membrane					1242:1249	AgNPs @chitosan membrane	1226:1249	AgNPs @chitosan membrane	1226:1249	Moreover, the amount of removed Imidacloprid was proportional with the amount of its initial concentration indicating the successful removal of Imidacloprid by AgNPs @chitosan membrane even at higher pesticide concentrations.
33309655	8	17	theme	pesticide	1266:1274	arg1	concentrations					1276:1289	higher pesticide concentrations	1259:1289	higher pesticide concentrations	1259:1289	Moreover, the amount of removed Imidacloprid was proportional with the amount of its initial concentration indicating the successful removal of Imidacloprid by AgNPs @chitosan membrane even at higher pesticide concentrations.
33309655	9	18	theme	external	1480:1487	arg1	support					1496:1502	external energy support	1480:1502	external energy support	1480:1502	The obtained results indicate the promising use of AgNPs @chitosan membranes for removal of Imidacloprid pesticide from contaminated water depending on the pressure-free design that lacks external energy support.
33309655	9	19	theme	chitosan	1350:1357	arg1	membranes					1359:1367	AgNPs @chitosan membranes	1343:1367	AgNPs @chitosan membranes	1343:1367	The obtained results indicate the promising use of AgNPs @chitosan membranes for removal of Imidacloprid pesticide from contaminated water depending on the pressure-free design that lacks external energy support.
33309655	9	20	theme	contaminated	1412:1423	arg1	water					1425:1429	contaminated water	1412:1429	contaminated water	1412:1429	The obtained results indicate the promising use of AgNPs @chitosan membranes for removal of Imidacloprid pesticide from contaminated water depending on the pressure-free design that lacks external energy support.
33309655	3	21	theme	pressure-free	465:477	arg1	passage					479:485	pressure-free passage	465:485	pressure-free passage through polymeric membrane integrated design	465:530	The current study is concerning by removal of Imidacloprid pesticide from water using pressure-free passage through polymeric membrane integrated design.
33309655	6	22	theme	silver	913:918	arg1	nanoparticles					920:932	silver nanoparticles	913:932	silver nanoparticles	913:932	However, SEM and FTIR analysis revealed the proper formation of chitosan membrane and its proper functionalization with silver nanoparticles.
33309655	6	23	theme	FTIR	810:813	arg1	analysis					815:822	SEM and FTIR analysis	802:822	analysis	815:822	However, SEM and FTIR analysis revealed the proper formation of chitosan membrane and its proper functionalization with silver nanoparticles.
33309655	1	24	theme	high	195:198	arg1	production					200:209	high production	195:209	high production of un-infected crops but higher environmental pollution	195:265	Wide dissemination of pesticides for protecting plants against pests has resulted in high production of un-infected crops but higher environmental pollution.
33309655	8	25	theme	Imidacloprid	1210:1221	arg1	removal					1199:1205	the successful removal	1184:1205	the successful removal of Imidacloprid by AgNPs @chitosan membrane	1184:1249	Moreover, the amount of removed Imidacloprid was proportional with the amount of its initial concentration indicating the successful removal of Imidacloprid by AgNPs @chitosan membrane even at higher pesticide concentrations.
33309655	4	26	theme	@	606:606	arg1	nanoparticles					585:597	functionalized silver nanoparticles	563:597	functionalized silver nanoparticles (AgNPs @chitosan)	563:615	Both of chitosan and chitosan functionalized silver nanoparticles (AgNPs @chitosan) membranes were prepared, characterized and applied as adsorbent matrix for Imidacloprid.
33309655	4	26	theme	@	606:606	arg1	chitosan					607:614	AgNPs @chitosan	600:614	AgNPs @chitosan	600:614	Both of chitosan and chitosan functionalized silver nanoparticles (AgNPs @chitosan) membranes were prepared, characterized and applied as adsorbent matrix for Imidacloprid.
33309655	7	27	theme	@	962:962	arg1	membranes					972:980	AgNPs @chitosan membranes	956:980	AgNPs @chitosan membranes	956:980	Both of chitosan and AgNPs @chitosan membranes succeeded to remove 40 and 85% of Imidacloprid at slightly acidic pH, respectively.
33309655	6	28	with	functionalization	890:906	arg1	nanoparticles					920:932	silver nanoparticles	913:932	silver nanoparticles	913:932	However, SEM and FTIR analysis revealed the proper formation of chitosan membrane and its proper functionalization with silver nanoparticles.
33309655	6	29	with	formation	844:852	arg1	nanoparticles					920:932	silver nanoparticles	913:932	silver nanoparticles	913:932	However, SEM and FTIR analysis revealed the proper formation of chitosan membrane and its proper functionalization with silver nanoparticles.
33309655	4	30	theme	AgNPs	600:604	arg1	nanoparticles					585:597	functionalized silver nanoparticles	563:597	functionalized silver nanoparticles (AgNPs @chitosan)	563:615	Both of chitosan and chitosan functionalized silver nanoparticles (AgNPs @chitosan) membranes were prepared, characterized and applied as adsorbent matrix for Imidacloprid.
33309655	4	30	theme	AgNPs	600:604	arg1	chitosan					607:614	AgNPs @chitosan	600:614	AgNPs @chitosan	600:614	Both of chitosan and chitosan functionalized silver nanoparticles (AgNPs @chitosan) membranes were prepared, characterized and applied as adsorbent matrix for Imidacloprid.
33309655	8	31	theme	Imidacloprid	1098:1109	arg1	proportional					1115:1126	proportional	1115:1126	proportional	1115:1126	Moreover, the amount of removed Imidacloprid was proportional with the amount of its initial concentration indicating the successful removal of Imidacloprid by AgNPs @chitosan membrane even at higher pesticide concentrations.
33309655	8	31	theme	Imidacloprid	1098:1109	arg1	amount					1080:1085	the amount	1076:1085	the amount of removed Imidacloprid	1076:1109	Moreover, the amount of removed Imidacloprid was proportional with the amount of its initial concentration indicating the successful removal of Imidacloprid by AgNPs @chitosan membrane even at higher pesticide concentrations.
33309655	8	31	theme	Imidacloprid	1098:1109	arg1	Imidacloprid					1098:1109	removed Imidacloprid	1090:1109	removed Imidacloprid	1090:1109	Moreover, the amount of removed Imidacloprid was proportional with the amount of its initial concentration indicating the successful removal of Imidacloprid by AgNPs @chitosan membrane even at higher pesticide concentrations.
33309655	6	32	theme	SEM	802:804	arg1	analysis					815:822	SEM and FTIR analysis	802:822	analysis	815:822	However, SEM and FTIR analysis revealed the proper formation of chitosan membrane and its proper functionalization with silver nanoparticles.
33309655	1	33	theme	Wide	110:113	arg1	dissemination					115:127	Wide dissemination	110:127	Wide dissemination of pesticides for protecting plants against pests	110:177	Wide dissemination of pesticides for protecting plants against pests has resulted in high production of un-infected crops but higher environmental pollution.
33309655	3	34	theme	polymeric	495:503	arg1	design					525:530	polymeric membrane integrated design	495:530	polymeric membrane integrated design	495:530	The current study is concerning by removal of Imidacloprid pesticide from water using pressure-free passage through polymeric membrane integrated design.
33309655	7	35	theme	Imidacloprid	1016:1027	arg1	Imidacloprid					1016:1027	Imidacloprid	1016:1027	Imidacloprid	1016:1027	Both of chitosan and AgNPs @chitosan membranes succeeded to remove 40 and 85% of Imidacloprid at slightly acidic pH, respectively.
33309655	7	35	theme	Imidacloprid	1016:1027	arg1	%					1011:1011	40 and 85%	1002:1011	%	1011:1011	Both of chitosan and AgNPs @chitosan membranes succeeded to remove 40 and 85% of Imidacloprid at slightly acidic pH, respectively.
33309655	8	36	theme	removed	1090:1096	arg1	Imidacloprid					1098:1109	removed Imidacloprid	1090:1109	removed Imidacloprid	1090:1109	Moreover, the amount of removed Imidacloprid was proportional with the amount of its initial concentration indicating the successful removal of Imidacloprid by AgNPs @chitosan membrane even at higher pesticide concentrations.
33309655	1	37	theme	un-infected	214:224	arg1	crops					226:230	un-infected crops	214:230	un-infected crops	214:230	Wide dissemination of pesticides for protecting plants against pests has resulted in high production of un-infected crops but higher environmental pollution.
33309655	3	38	theme	Imidacloprid	425:436	arg1	pesticide					438:446	Imidacloprid pesticide	425:446	Imidacloprid pesticide	425:446	The current study is concerning by removal of Imidacloprid pesticide from water using pressure-free passage through polymeric membrane integrated design.
33309655	9	39	theme	Imidacloprid	1384:1395	arg1	pesticide					1397:1405	Imidacloprid pesticide	1384:1405	Imidacloprid pesticide	1384:1405	The obtained results indicate the promising use of AgNPs @chitosan membranes for removal of Imidacloprid pesticide from contaminated water depending on the pressure-free design that lacks external energy support.
33309655	1	40	theme	crops	226:230	arg1	production					200:209	high production	195:209	high production of un-infected crops but higher environmental pollution	195:265	Wide dissemination of pesticides for protecting plants against pests has resulted in high production of un-infected crops but higher environmental pollution.
33309655	0	41	theme	efficient	34:42	arg1	removal					44:50	efficient removal	34:50	efficient removal of Imidacloprid pesticide	34:76	Chitosan functionalized AgNPs for efficient removal of Imidacloprid pesticide through a pressure-free design.
33309655	4	42	theme	silver	578:583	arg1	nanoparticles					585:597	functionalized silver nanoparticles	563:597	functionalized silver nanoparticles (AgNPs @chitosan)	563:615	Both of chitosan and chitosan functionalized silver nanoparticles (AgNPs @chitosan) membranes were prepared, characterized and applied as adsorbent matrix for Imidacloprid.
33309655	4	42	theme	silver	578:583	arg1	chitosan					607:614	AgNPs @chitosan	600:614	AgNPs @chitosan	600:614	Both of chitosan and chitosan functionalized silver nanoparticles (AgNPs @chitosan) membranes were prepared, characterized and applied as adsorbent matrix for Imidacloprid.
33309655	3	43	theme	pesticide	438:446	arg1	removal					414:420	removal	414:420	removal of Imidacloprid pesticide from water using pressure-free passage through polymeric membrane integrated design	414:530	The current study is concerning by removal of Imidacloprid pesticide from water using pressure-free passage through polymeric membrane integrated design.
33309655	6	44	theme	proper	883:888	arg1	functionalization					890:906	its proper functionalization	879:906	its proper functionalization with silver nanoparticles	879:932	However, SEM and FTIR analysis revealed the proper formation of chitosan membrane and its proper functionalization with silver nanoparticles.
33309655	8	45	theme	@	1232:1232	arg1	membrane					1242:1249	AgNPs @chitosan membrane	1226:1249	AgNPs @chitosan membrane	1226:1249	Moreover, the amount of removed Imidacloprid was proportional with the amount of its initial concentration indicating the successful removal of Imidacloprid by AgNPs @chitosan membrane even at higher pesticide concentrations.
33309655	7	46	theme	acidic	1041:1046	arg1	pH					1048:1049	slightly acidic pH	1032:1049	slightly acidic pH	1032:1049	Both of chitosan and AgNPs @chitosan membranes succeeded to remove 40 and 85% of Imidacloprid at slightly acidic pH, respectively.
33309655	0	47	theme	Imidacloprid	55:66	arg1	pesticide					68:76	Imidacloprid pesticide	55:76	Imidacloprid pesticide	55:76	Chitosan functionalized AgNPs for efficient removal of Imidacloprid pesticide through a pressure-free design.
33309655	4	48	theme	functionalized	563:576	arg1	nanoparticles					585:597	functionalized silver nanoparticles	563:597	functionalized silver nanoparticles (AgNPs @chitosan)	563:615	Both of chitosan and chitosan functionalized silver nanoparticles (AgNPs @chitosan) membranes were prepared, characterized and applied as adsorbent matrix for Imidacloprid.
33309655	4	48	theme	functionalized	563:576	arg1	chitosan					607:614	AgNPs @chitosan	600:614	AgNPs @chitosan	600:614	Both of chitosan and chitosan functionalized silver nanoparticles (AgNPs @chitosan) membranes were prepared, characterized and applied as adsorbent matrix for Imidacloprid.
33309655	8	49	theme	chitosan	1233:1240	arg1	membrane					1242:1249	AgNPs @chitosan membrane	1226:1249	AgNPs @chitosan membrane	1226:1249	Moreover, the amount of removed Imidacloprid was proportional with the amount of its initial concentration indicating the successful removal of Imidacloprid by AgNPs @chitosan membrane even at higher pesticide concentrations.
33309655	9	50	from	water	1425:1429	arg1	removal					1373:1379	removal	1373:1379	removal of Imidacloprid pesticide from contaminated water	1373:1429	The obtained results indicate the promising use of AgNPs @chitosan membranes for removal of Imidacloprid pesticide from contaminated water depending on the pressure-free design that lacks external energy support.
33309655	9	51	theme	energy	1489:1494	arg1	support					1496:1502	external energy support	1480:1502	external energy support	1480:1502	The obtained results indicate the promising use of AgNPs @chitosan membranes for removal of Imidacloprid pesticide from contaminated water depending on the pressure-free design that lacks external energy support.
33309655	2	52	theme	final	360:364	arg1	water					347:351	water	347:351	water	347:351	High percentages of pesticides are released to the environment and finally use water as the final destination.
33309655	2	52	theme	final	360:364	arg1	destination					366:376	the final destination	356:376	the final destination	356:376	High percentages of pesticides are released to the environment and finally use water as the final destination.
33309655	5	53	theme	AgNPs	761:765	arg1	biosynthesis					745:756	the biosynthesis	741:756	the biosynthesis of AgNPs in the range of 25-50 nm	741:790	SEM, TEM and PSA analysis revealed the biosynthesis of AgNPs in the range of 25-50 nm.
33309655	4	54	dep	chitosan	541:548	arg1	membranes					617:625	membranes	617:625	membranes	617:625	Both of chitosan and chitosan functionalized silver nanoparticles (AgNPs @chitosan) membranes were prepared, characterized and applied as adsorbent matrix for Imidacloprid.
33309655	5	55	theme	PSA	719:721	arg1	analysis					723:730	SEM, TEM and PSA analysis	706:730	SEM, TEM and PSA analysis	706:730	SEM, TEM and PSA analysis revealed the biosynthesis of AgNPs in the range of 25-50 nm.
33309655	9	56	theme	obtained	1296:1303	arg1	results					1305:1311	The obtained results	1292:1311	The obtained results	1292:1311	The obtained results indicate the promising use of AgNPs @chitosan membranes for removal of Imidacloprid pesticide from contaminated water depending on the pressure-free design that lacks external energy support.
33309655	9	57	theme	promising	1326:1334	arg1	use					1336:1338	the promising use	1322:1338	the promising use of AgNPs @chitosan membranes for removal of Imidacloprid pesticide from contaminated water	1322:1429	The obtained results indicate the promising use of AgNPs @chitosan membranes for removal of Imidacloprid pesticide from contaminated water depending on the pressure-free design that lacks external energy support.
33309655	1	58	theme	pesticides	132:141	arg1	dissemination					115:127	Wide dissemination	110:127	Wide dissemination of pesticides for protecting plants against pests	110:177	Wide dissemination of pesticides for protecting plants against pests has resulted in high production of un-infected crops but higher environmental pollution.
33309655	8	59	with	proportional	1115:1126	arg1	concentration					1159:1171	its initial concentration	1147:1171	its initial concentration	1147:1171	Moreover, the amount of removed Imidacloprid was proportional with the amount of its initial concentration indicating the successful removal of Imidacloprid by AgNPs @chitosan membrane even at higher pesticide concentrations.
33309655	8	59	with	proportional	1115:1126	arg1	amount					1137:1142	the amount	1133:1142	the amount of its initial concentration indicating the successful removal of Imidacloprid by AgNPs @chitosan membrane even at higher pesticide concentrations	1133:1289	Moreover, the amount of removed Imidacloprid was proportional with the amount of its initial concentration indicating the successful removal of Imidacloprid by AgNPs @chitosan membrane even at higher pesticide concentrations.
33309655	7	60	theme	chitosan	963:970	arg1	membranes					972:980	AgNPs @chitosan membranes	956:980	AgNPs @chitosan membranes	956:980	Both of chitosan and AgNPs @chitosan membranes succeeded to remove 40 and 85% of Imidacloprid at slightly acidic pH, respectively.
33309655	6	61	theme	membrane	866:873	arg1	functionalization					890:906	its proper functionalization	879:906	its proper functionalization with silver nanoparticles	879:932	However, SEM and FTIR analysis revealed the proper formation of chitosan membrane and its proper functionalization with silver nanoparticles.
33309655	6	61	theme	membrane	866:873	arg1	formation					844:852	the proper formation	833:852	the proper formation of chitosan membrane	833:873	However, SEM and FTIR analysis revealed the proper formation of chitosan membrane and its proper functionalization with silver nanoparticles.
33309655	2	62	theme	pesticides	288:297	arg1	percentages					273:283	High percentages	268:283	High percentages of pesticides	268:297	High percentages of pesticides are released to the environment and finally use water as the final destination.
33309655	3	63	theme	integrated	514:523	arg1	design					525:530	polymeric membrane integrated design	495:530	polymeric membrane integrated design	495:530	The current study is concerning by removal of Imidacloprid pesticide from water using pressure-free passage through polymeric membrane integrated design.
33309655	7	64	theme	AgNPs	956:960	arg1	membranes					972:980	AgNPs @chitosan membranes	956:980	AgNPs @chitosan membranes	956:980	Both of chitosan and AgNPs @chitosan membranes succeeded to remove 40 and 85% of Imidacloprid at slightly acidic pH, respectively.
33309655	1	65	theme	higher	236:241	arg1	pollution					257:265	higher environmental pollution	236:265	higher environmental pollution	236:265	Wide dissemination of pesticides for protecting plants against pests has resulted in high production of un-infected crops but higher environmental pollution.
33309655	6	66	theme	chitosan	857:864	arg1	membrane					866:873	chitosan membrane	857:873	chitosan membrane	857:873	However, SEM and FTIR analysis revealed the proper formation of chitosan membrane and its proper functionalization with silver nanoparticles.
33309655	9	67	theme	pesticide	1397:1405	arg1	removal					1373:1379	removal	1373:1379	removal of Imidacloprid pesticide from contaminated water	1373:1429	The obtained results indicate the promising use of AgNPs @chitosan membranes for removal of Imidacloprid pesticide from contaminated water depending on the pressure-free design that lacks external energy support.
33309655	1	68	theme	environmental	243:255	arg1	pollution					257:265	higher environmental pollution	236:265	higher environmental pollution	236:265	Wide dissemination of pesticides for protecting plants against pests has resulted in high production of un-infected crops but higher environmental pollution.
33309655	5	69	theme	25-50 nm	783:790	arg1	range					774:778	the range	770:778	the range of 25-50 nm	770:790	SEM, TEM and PSA analysis revealed the biosynthesis of AgNPs in the range of 25-50 nm.
33309655	3	70	from	water	453:457	arg1	removal					414:420	removal	414:420	removal of Imidacloprid pesticide from water using pressure-free passage through polymeric membrane integrated design	414:530	The current study is concerning by removal of Imidacloprid pesticide from water using pressure-free passage through polymeric membrane integrated design.
33309655	8	71	theme	higher	1259:1264	arg1	concentrations					1276:1289	higher pesticide concentrations	1259:1289	higher pesticide concentrations	1259:1289	Moreover, the amount of removed Imidacloprid was proportional with the amount of its initial concentration indicating the successful removal of Imidacloprid by AgNPs @chitosan membrane even at higher pesticide concentrations.
33309655	1	72	theme	pollution	257:265	arg1	production					200:209	high production	195:209	high production of un-infected crops but higher environmental pollution	195:265	Wide dissemination of pesticides for protecting plants against pests has resulted in high production of un-infected crops but higher environmental pollution.
33309655	0	73	theme	pesticide	68:76	arg1	removal					44:50	efficient removal	34:50	efficient removal of Imidacloprid pesticide	34:76	Chitosan functionalized AgNPs for efficient removal of Imidacloprid pesticide through a pressure-free design.
33309655	2	74	theme	High	268:271	arg1	percentages					273:283	High percentages	268:283	High percentages of pesticides	268:297	High percentages of pesticides are released to the environment and finally use water as the final destination.
33309655	7	75	theme	85	1009:1010	arg1	Imidacloprid					1016:1027	Imidacloprid	1016:1027	Imidacloprid	1016:1027	Both of chitosan and AgNPs @chitosan membranes succeeded to remove 40 and 85% of Imidacloprid at slightly acidic pH, respectively.
33309655	7	75	theme	85	1009:1010	arg1	%					1011:1011	40 and 85%	1002:1011	%	1011:1011	Both of chitosan and AgNPs @chitosan membranes succeeded to remove 40 and 85% of Imidacloprid at slightly acidic pH, respectively.
33309655	9	76	theme	pressure-free	1448:1460	arg1	design					1462:1467	the pressure-free design	1444:1467	the pressure-free design that lacks external energy support	1444:1502	The obtained results indicate the promising use of AgNPs @chitosan membranes for removal of Imidacloprid pesticide from contaminated water depending on the pressure-free design that lacks external energy support.
33249594	5	0	theme	Ng	645:646	arg1	present					648:654	Ng present	645:654	Ng present in CSF	645:661	To obtain an overview of the different molecular forms of Ng present in CSF, we show by size exclusion chromatography (SEC), immunoblotting, immunoprecipitation, and MS that Ng is present in CSF as several molecular forms.
33249594	6	1	theme	N-terminal	932:941	arg1	fragments					943:951	C-terminal- and previously not identified N-terminal fragments	890:951	C-terminal- and previously not identified N-terminal fragments	890:951	Besides monomeric full-length Ng, also higher molecular weight forms of Ng, and C-terminal- and previously not identified N-terminal fragments were observed.
33249594	10	2	from	presence	1442:1449	arg1	CSF					1487:1489	CSF	1487:1489	CSF	1487:1489	Taken together, this study highlights the presence of several molecular forms of Ng in CSF, comprising monomeric full-length Ng, and N- and C-terminal truncations of Ng, as well as larger forms of still unknown composition.
33249594	9	3	theme	monomeric	1244:1252	arg1	Ng					1254:1255	monomeric Ng	1244:1255	monomeric Ng	1244:1255	In addition, we found that monomeric Ng and its C-terminal fragments bind to heparin via a heparin-binding motif, which might be of relevance for their export mechanism from neurons.
33249594	5	4	theme	present	648:654	arg1	forms					636:640	the different molecular forms	612:640	the different molecular forms of Ng present in CSF	612:661	To obtain an overview of the different molecular forms of Ng present in CSF, we show by size exclusion chromatography (SEC), immunoblotting, immunoprecipitation, and MS that Ng is present in CSF as several molecular forms.
33249594	0	5	from	forms	10:14	arg1	fluid					48:52	cerebrospinal fluid	34:52	cerebrospinal fluid	34:52	Molecular forms of neurogranin in cerebrospinal fluid.
33249594	6	6	theme	identified	921:930	arg1	fragments					943:951	C-terminal- and previously not identified N-terminal fragments	890:951	C-terminal- and previously not identified N-terminal fragments	890:951	Besides monomeric full-length Ng, also higher molecular weight forms of Ng, and C-terminal- and previously not identified N-terminal fragments were observed.
33249594	8	7	theme	fragment/total-Ng	1148:1164	arg1	ratios					1166:1171	the overall C-terminal fragment/total-Ng ratios	1125:1171	the overall C-terminal fragment/total-Ng ratios	1125:1171	There were no differences in the overall C-terminal fragment/total-Ng ratios between samples from AD and control groups.
33249594	5	8	attach	present	767:773	arg1	CSF					778:780	CSF	778:780	CSF	778:780	To obtain an overview of the different molecular forms of Ng present in CSF, we show by size exclusion chromatography (SEC), immunoblotting, immunoprecipitation, and MS that Ng is present in CSF as several molecular forms.
33249594	5	8	attach	present	767:773	arg2	Ng					761:762	Ng	761:762	Ng	761:762	To obtain an overview of the different molecular forms of Ng present in CSF, we show by size exclusion chromatography (SEC), immunoblotting, immunoprecipitation, and MS that Ng is present in CSF as several molecular forms.
33249594	7	9	from	samples	1087:1093	arg1	%					1049:1049	~50%	1046:1049	~50% of the total-Ng ELISA signal in CSF samples	1046:1093	We found by immunodepletion that C-terminal peptides contribute on average to ~50% of the total-Ng ELISA signal in CSF samples.
33249594	7	9	from	samples	1087:1093	arg1	signal					1073:1078	the total-Ng ELISA signal	1054:1078	the total-Ng ELISA signal in CSF samples	1054:1093	We found by immunodepletion that C-terminal peptides contribute on average to ~50% of the total-Ng ELISA signal in CSF samples.
33249594	5	10	theme	molecular	793:801	arg1	forms					803:807	several molecular forms	785:807	several molecular forms	785:807	To obtain an overview of the different molecular forms of Ng present in CSF, we show by size exclusion chromatography (SEC), immunoblotting, immunoprecipitation, and MS that Ng is present in CSF as several molecular forms.
33249594	5	11	from	CSF	659:661	arg1	forms					636:640	the different molecular forms	612:640	the different molecular forms of Ng present in CSF	612:661	To obtain an overview of the different molecular forms of Ng present in CSF, we show by size exclusion chromatography (SEC), immunoblotting, immunoprecipitation, and MS that Ng is present in CSF as several molecular forms.
33249594	3	12	theme	important	306:314	arg1	domain					316:321	this functionally important domain	288:321	this functionally important domain	288:321	Ng is cleaved within this functionally important domain, yielding the majority of fragments identified in cerebrospinal fluid (CSF), suggesting that cleavage of Ng may be a mechanism to regulate its function.
33249594	4	13	dep	now	482:484	arg1	Up					476:477	Up	476:477	Up	476:477	Up to now, Ng has been shown to be present in CSF as both C-terminal fragments as well as full-length protein.
33249594	10	14	theme	Ng	1566:1567	arg1	Ng					1525:1526	monomeric full-length Ng	1503:1526	monomeric full-length Ng	1503:1526	Taken together, this study highlights the presence of several molecular forms of Ng in CSF, comprising monomeric full-length Ng, and N- and C-terminal truncations of Ng, as well as larger forms of still unknown composition.
33249594	10	14	theme	Ng	1566:1567	arg1	N-					1533:1534	N-	1533:1534	N-	1533:1534	Taken together, this study highlights the presence of several molecular forms of Ng in CSF, comprising monomeric full-length Ng, and N- and C-terminal truncations of Ng, as well as larger forms of still unknown composition.
33249594	10	14	theme	Ng	1566:1567	arg1	truncations					1551:1561	C-terminal truncations	1540:1561	C-terminal truncations	1540:1561	Taken together, this study highlights the presence of several molecular forms of Ng in CSF, comprising monomeric full-length Ng, and N- and C-terminal truncations of Ng, as well as larger forms of still unknown composition.
33249594	7	15	theme	CSF	1083:1085	arg1	samples					1087:1093	CSF samples	1083:1093	CSF samples	1083:1093	We found by immunodepletion that C-terminal peptides contribute on average to ~50% of the total-Ng ELISA signal in CSF samples.
33249594	9	16	theme	export	1369:1374	arg1	mechanism					1376:1384	their export mechanism	1363:1384	their export mechanism from neurons	1363:1397	In addition, we found that monomeric Ng and its C-terminal fragments bind to heparin via a heparin-binding motif, which might be of relevance for their export mechanism from neurons.
33249594	6	17	theme	full-length	828:838	arg1	Ng					840:841	monomeric full-length Ng	818:841	monomeric full-length Ng	818:841	Besides monomeric full-length Ng, also higher molecular weight forms of Ng, and C-terminal- and previously not identified N-terminal fragments were observed.
33249594	5	18	theme	different	616:624	arg1	forms					636:640	the different molecular forms	612:640	the different molecular forms of Ng present in CSF	612:661	To obtain an overview of the different molecular forms of Ng present in CSF, we show by size exclusion chromatography (SEC), immunoblotting, immunoprecipitation, and MS that Ng is present in CSF as several molecular forms.
33249594	0	19	from	fluid	48:52	arg1	forms					10:14	Molecular forms	0:14	Molecular forms of neurogranin in cerebrospinal fluid	0:52	Molecular forms of neurogranin in cerebrospinal fluid.
33249594	8	20	theme	control	1201:1207	arg1	groups					1209:1214	control groups	1201:1214	control groups	1201:1214	There were no differences in the overall C-terminal fragment/total-Ng ratios between samples from AD and control groups.
33249594	8	21	from	AD	1194:1195	arg1	samples					1181:1187	samples	1181:1187	samples from AD and control groups	1181:1214	There were no differences in the overall C-terminal fragment/total-Ng ratios between samples from AD and control groups.
33249594	9	22	theme	C-terminal	1265:1274	arg1	fragments					1276:1284	its C-terminal fragments	1261:1284	its C-terminal fragments	1261:1284	In addition, we found that monomeric Ng and its C-terminal fragments bind to heparin via a heparin-binding motif, which might be of relevance for their export mechanism from neurons.
33249594	5	23	theme	molecular	626:634	arg1	forms					636:640	the different molecular forms	612:640	the different molecular forms of Ng present in CSF	612:661	To obtain an overview of the different molecular forms of Ng present in CSF, we show by size exclusion chromatography (SEC), immunoblotting, immunoprecipitation, and MS that Ng is present in CSF as several molecular forms.
33249594	10	24	theme	full-length	1513:1523	arg1	Ng					1525:1526	monomeric full-length Ng	1503:1526	monomeric full-length Ng	1503:1526	Taken together, this study highlights the presence of several molecular forms of Ng in CSF, comprising monomeric full-length Ng, and N- and C-terminal truncations of Ng, as well as larger forms of still unknown composition.
33249594	10	24	theme	full-length	1513:1523	arg1	N-					1533:1534	N-	1533:1534	N-	1533:1534	Taken together, this study highlights the presence of several molecular forms of Ng in CSF, comprising monomeric full-length Ng, and N- and C-terminal truncations of Ng, as well as larger forms of still unknown composition.
33249594	10	24	theme	full-length	1513:1523	arg1	truncations					1551:1561	C-terminal truncations	1540:1561	C-terminal truncations	1540:1561	Taken together, this study highlights the presence of several molecular forms of Ng in CSF, comprising monomeric full-length Ng, and N- and C-terminal truncations of Ng, as well as larger forms of still unknown composition.
33249594	0	25	theme	cerebrospinal	34:46	arg1	fluid					48:52	cerebrospinal fluid	34:52	cerebrospinal fluid	34:52	Molecular forms of neurogranin in cerebrospinal fluid.
33249594	5	26	from	present	767:773	arg1	CSF					778:780	CSF	778:780	CSF	778:780	To obtain an overview of the different molecular forms of Ng present in CSF, we show by size exclusion chromatography (SEC), immunoblotting, immunoprecipitation, and MS that Ng is present in CSF as several molecular forms.
33249594	2	27	theme	phosphatidic	212:223	arg1	acid					225:228	phosphatidic acid	212:228	phosphatidic acid	212:228	Ng has been suggested to bind to calmodulin and phosphatidic acid via its centrally located IQ domain.
33249594	4	28	theme	C-terminal	534:543	arg1	fragments					545:553	both C-terminal fragments	529:553	both C-terminal fragments as well as full-length protein	529:584	Up to now, Ng has been shown to be present in CSF as both C-terminal fragments as well as full-length protein.
33249594	10	29	attach	presence	1442:1449	arg1	CSF					1487:1489	CSF	1487:1489	CSF	1487:1489	Taken together, this study highlights the presence of several molecular forms of Ng in CSF, comprising monomeric full-length Ng, and N- and C-terminal truncations of Ng, as well as larger forms of still unknown composition.
33249594	10	29	attach	presence	1442:1449	arg2	forms					1472:1476	several molecular forms	1454:1476	several molecular forms of Ng	1454:1482	Taken together, this study highlights the presence of several molecular forms of Ng in CSF, comprising monomeric full-length Ng, and N- and C-terminal truncations of Ng, as well as larger forms of still unknown composition.
33249594	7	30	theme	total-Ng	1058:1065	arg1	signal					1073:1078	the total-Ng ELISA signal	1054:1078	the total-Ng ELISA signal in CSF samples	1054:1093	We found by immunodepletion that C-terminal peptides contribute on average to ~50% of the total-Ng ELISA signal in CSF samples.
33249594	5	31	theme	forms	636:640	arg1	overview					600:607	an overview	597:607	an overview of the different molecular forms of Ng present in CSF	597:661	To obtain an overview of the different molecular forms of Ng present in CSF, we show by size exclusion chromatography (SEC), immunoblotting, immunoprecipitation, and MS that Ng is present in CSF as several molecular forms.
33249594	10	32	theme	larger	1581:1586	arg1	forms					1588:1592	larger forms	1581:1592	larger forms of still unknown composition	1581:1621	Taken together, this study highlights the presence of several molecular forms of Ng in CSF, comprising monomeric full-length Ng, and N- and C-terminal truncations of Ng, as well as larger forms of still unknown composition.
33249594	10	33	theme	forms	1472:1476	arg1	presence					1442:1449	the presence	1438:1449	the presence of several molecular forms of Ng in CSF	1438:1489	Taken together, this study highlights the presence of several molecular forms of Ng in CSF, comprising monomeric full-length Ng, and N- and C-terminal truncations of Ng, as well as larger forms of still unknown composition.
33249594	0	34	theme	Molecular	0:8	arg1	forms					10:14	Molecular forms	0:14	Molecular forms of neurogranin in cerebrospinal fluid	0:52	Molecular forms of neurogranin in cerebrospinal fluid.
33249594	6	35	theme	monomeric	818:826	arg1	Ng					840:841	monomeric full-length Ng	818:841	monomeric full-length Ng	818:841	Besides monomeric full-length Ng, also higher molecular weight forms of Ng, and C-terminal- and previously not identified N-terminal fragments were observed.
33249594	10	36	theme	monomeric	1503:1511	arg1	Ng					1525:1526	monomeric full-length Ng	1503:1526	monomeric full-length Ng	1503:1526	Taken together, this study highlights the presence of several molecular forms of Ng in CSF, comprising monomeric full-length Ng, and N- and C-terminal truncations of Ng, as well as larger forms of still unknown composition.
33249594	10	36	theme	monomeric	1503:1511	arg1	N-					1533:1534	N-	1533:1534	N-	1533:1534	Taken together, this study highlights the presence of several molecular forms of Ng in CSF, comprising monomeric full-length Ng, and N- and C-terminal truncations of Ng, as well as larger forms of still unknown composition.
33249594	10	36	theme	monomeric	1503:1511	arg1	truncations					1551:1561	C-terminal truncations	1540:1561	C-terminal truncations	1540:1561	Taken together, this study highlights the presence of several molecular forms of Ng in CSF, comprising monomeric full-length Ng, and N- and C-terminal truncations of Ng, as well as larger forms of still unknown composition.
33249594	2	37	theme	IQ	256:257	arg1	domain					259:264	its centrally located IQ domain	234:264	its centrally located IQ domain	234:264	Ng has been suggested to bind to calmodulin and phosphatidic acid via its centrally located IQ domain.
33249594	3	38	theme	cerebrospinal	373:385	arg1	fluid					387:391	cerebrospinal fluid	373:391	cerebrospinal fluid (CSF)	373:397	Ng is cleaved within this functionally important domain, yielding the majority of fragments identified in cerebrospinal fluid (CSF), suggesting that cleavage of Ng may be a mechanism to regulate its function.
33249594	3	38	theme	cerebrospinal	373:385	arg1	CSF					394:396	CSF	394:396	CSF	394:396	Ng is cleaved within this functionally important domain, yielding the majority of fragments identified in cerebrospinal fluid (CSF), suggesting that cleavage of Ng may be a mechanism to regulate its function.
33249594	0	39	theme	neurogranin	19:29	arg1	forms					10:14	Molecular forms	0:14	Molecular forms of neurogranin in cerebrospinal fluid	0:52	Molecular forms of neurogranin in cerebrospinal fluid.
33249594	9	40	from	neurons	1391:1397	arg1	mechanism					1376:1384	their export mechanism	1363:1384	their export mechanism from neurons	1363:1397	In addition, we found that monomeric Ng and its C-terminal fragments bind to heparin via a heparin-binding motif, which might be of relevance for their export mechanism from neurons.
33249594	4	41	attach	present	511:517	arg1	CSF					522:524	CSF	522:524	CSF	522:524	Up to now, Ng has been shown to be present in CSF as both C-terminal fragments as well as full-length protein.
33249594	4	41	attach	present	511:517	arg2	Ng					487:488	Ng	487:488	Ng	487:488	Up to now, Ng has been shown to be present in CSF as both C-terminal fragments as well as full-length protein.
33249594	2	42	theme	located	248:254	arg1	domain					259:264	its centrally located IQ domain	234:264	its centrally located IQ domain	234:264	Ng has been suggested to bind to calmodulin and phosphatidic acid via its centrally located IQ domain.
33249594	5	43	theme	exclusion	680:688	arg1	SEC					706:708	SEC	706:708	SEC	706:708	To obtain an overview of the different molecular forms of Ng present in CSF, we show by size exclusion chromatography (SEC), immunoblotting, immunoprecipitation, and MS that Ng is present in CSF as several molecular forms.
33249594	5	43	theme	exclusion	680:688	arg1	chromatography					690:703	size exclusion chromatography	675:703	size exclusion chromatography (SEC)	675:709	To obtain an overview of the different molecular forms of Ng present in CSF, we show by size exclusion chromatography (SEC), immunoblotting, immunoprecipitation, and MS that Ng is present in CSF as several molecular forms.
33249594	1	44	theme	amino	80:84	arg1	protein					100:106	a 78 amino acid neuronal protein	75:106	a 78 amino acid neuronal protein	75:106	Neurogranin (Ng) is a 78 amino acid neuronal protein and a biomarker candidate for Alzheimer's disease (AD).
33249594	1	44	theme	amino	80:84	arg1	Neurogranin					55:65	Neurogranin	55:65	Neurogranin (Ng)	55:70	Neurogranin (Ng) is a 78 amino acid neuronal protein and a biomarker candidate for Alzheimer's disease (AD).
33249594	4	45	from	CSF	522:524	arg1	present					511:517	present	511:517	present	511:517	Up to now, Ng has been shown to be present in CSF as both C-terminal fragments as well as full-length protein.
33249594	10	46	theme	C-terminal	1540:1549	arg1	Ng					1525:1526	monomeric full-length Ng	1503:1526	monomeric full-length Ng	1503:1526	Taken together, this study highlights the presence of several molecular forms of Ng in CSF, comprising monomeric full-length Ng, and N- and C-terminal truncations of Ng, as well as larger forms of still unknown composition.
33249594	10	46	theme	C-terminal	1540:1549	arg1	truncations					1551:1561	C-terminal truncations	1540:1561	C-terminal truncations	1540:1561	Taken together, this study highlights the presence of several molecular forms of Ng in CSF, comprising monomeric full-length Ng, and N- and C-terminal truncations of Ng, as well as larger forms of still unknown composition.
33249594	3	47	theme	fragments	349:357	arg1	majority					337:344	the majority	333:344	the majority of fragments identified in cerebrospinal fluid (CSF)	333:397	Ng is cleaved within this functionally important domain, yielding the majority of fragments identified in cerebrospinal fluid (CSF), suggesting that cleavage of Ng may be a mechanism to regulate its function.
33249594	3	48	theme	Ng	428:429	arg1	cleavage					416:423	cleavage	416:423	cleavage of Ng	416:429	Ng is cleaved within this functionally important domain, yielding the majority of fragments identified in cerebrospinal fluid (CSF), suggesting that cleavage of Ng may be a mechanism to regulate its function.
33249594	3	48	theme	Ng	428:429	arg1	mechanism					440:448	a mechanism	438:448	a mechanism to regulate its function	438:473	Ng is cleaved within this functionally important domain, yielding the majority of fragments identified in cerebrospinal fluid (CSF), suggesting that cleavage of Ng may be a mechanism to regulate its function.
33249594	8	49	from	groups	1209:1214	arg1	samples					1181:1187	samples	1181:1187	samples from AD and control groups	1181:1214	There were no differences in the overall C-terminal fragment/total-Ng ratios between samples from AD and control groups.
33249594	10	50	theme	unknown	1603:1609	arg1	composition					1611:1621	still unknown composition	1597:1621	still unknown composition	1597:1621	Taken together, this study highlights the presence of several molecular forms of Ng in CSF, comprising monomeric full-length Ng, and N- and C-terminal truncations of Ng, as well as larger forms of still unknown composition.
33249594	6	51	theme	C-terminal-	890:900	arg1	fragments					943:951	C-terminal- and previously not identified N-terminal fragments	890:951	C-terminal- and previously not identified N-terminal fragments	890:951	Besides monomeric full-length Ng, also higher molecular weight forms of Ng, and C-terminal- and previously not identified N-terminal fragments were observed.
33249594	6	52	theme	Ng	882:883	arg1	fragments					943:951	C-terminal- and previously not identified N-terminal fragments	890:951	C-terminal- and previously not identified N-terminal fragments	890:951	Besides monomeric full-length Ng, also higher molecular weight forms of Ng, and C-terminal- and previously not identified N-terminal fragments were observed.
33249594	6	52	theme	Ng	882:883	arg1	forms					873:877	higher molecular weight forms	849:877	higher molecular weight forms of Ng	849:883	Besides monomeric full-length Ng, also higher molecular weight forms of Ng, and C-terminal- and previously not identified N-terminal fragments were observed.
33249594	7	53	theme	C-terminal	1001:1010	arg1	peptides					1012:1019	C-terminal peptides	1001:1019	C-terminal peptides	1001:1019	We found by immunodepletion that C-terminal peptides contribute on average to ~50% of the total-Ng ELISA signal in CSF samples.
33249594	10	54	theme	Ng	1481:1482	arg1	forms					1472:1476	several molecular forms	1454:1476	several molecular forms of Ng	1454:1482	Taken together, this study highlights the presence of several molecular forms of Ng in CSF, comprising monomeric full-length Ng, and N- and C-terminal truncations of Ng, as well as larger forms of still unknown composition.
33249594	7	55	from	%	1049:1049	arg1	samples					1087:1093	CSF samples	1083:1093	CSF samples	1083:1093	We found by immunodepletion that C-terminal peptides contribute on average to ~50% of the total-Ng ELISA signal in CSF samples.
33249594	5	56	theme	size	675:678	arg1	SEC					706:708	SEC	706:708	SEC	706:708	To obtain an overview of the different molecular forms of Ng present in CSF, we show by size exclusion chromatography (SEC), immunoblotting, immunoprecipitation, and MS that Ng is present in CSF as several molecular forms.
33249594	5	56	theme	size	675:678	arg1	chromatography					690:703	size exclusion chromatography	675:703	size exclusion chromatography (SEC)	675:709	To obtain an overview of the different molecular forms of Ng present in CSF, we show by size exclusion chromatography (SEC), immunoblotting, immunoprecipitation, and MS that Ng is present in CSF as several molecular forms.
33249594	9	57	theme	heparin-binding	1308:1322	arg1	motif					1324:1328	a heparin-binding motif	1306:1328	a heparin-binding motif	1306:1328	In addition, we found that monomeric Ng and its C-terminal fragments bind to heparin via a heparin-binding motif, which might be of relevance for their export mechanism from neurons.
33249594	10	58	theme	molecular	1462:1470	arg1	forms					1472:1476	several molecular forms	1454:1476	several molecular forms of Ng	1454:1482	Taken together, this study highlights the presence of several molecular forms of Ng in CSF, comprising monomeric full-length Ng, and N- and C-terminal truncations of Ng, as well as larger forms of still unknown composition.
33249594	1	59	theme	acid	86:89	arg1	protein					100:106	a 78 amino acid neuronal protein	75:106	a 78 amino acid neuronal protein	75:106	Neurogranin (Ng) is a 78 amino acid neuronal protein and a biomarker candidate for Alzheimer's disease (AD).
33249594	1	59	theme	acid	86:89	arg1	Neurogranin					55:65	Neurogranin	55:65	Neurogranin (Ng)	55:70	Neurogranin (Ng) is a 78 amino acid neuronal protein and a biomarker candidate for Alzheimer's disease (AD).
33249594	5	60	located	present	767:773	arg1	CSF					778:780	CSF	778:780	CSF	778:780	To obtain an overview of the different molecular forms of Ng present in CSF, we show by size exclusion chromatography (SEC), immunoblotting, immunoprecipitation, and MS that Ng is present in CSF as several molecular forms.
33249594	5	60	located	present	767:773	arg2	Ng					761:762	Ng	761:762	Ng	761:762	To obtain an overview of the different molecular forms of Ng present in CSF, we show by size exclusion chromatography (SEC), immunoblotting, immunoprecipitation, and MS that Ng is present in CSF as several molecular forms.
33249594	7	61	theme	ELISA	1067:1071	arg1	signal					1073:1078	the total-Ng ELISA signal	1054:1078	the total-Ng ELISA signal in CSF samples	1054:1093	We found by immunodepletion that C-terminal peptides contribute on average to ~50% of the total-Ng ELISA signal in CSF samples.
33249594	4	62	located	present	511:517	arg1	CSF					522:524	CSF	522:524	CSF	522:524	Up to now, Ng has been shown to be present in CSF as both C-terminal fragments as well as full-length protein.
33249594	4	62	located	present	511:517	arg2	Ng					487:488	Ng	487:488	Ng	487:488	Up to now, Ng has been shown to be present in CSF as both C-terminal fragments as well as full-length protein.
33249594	1	63	theme	neuronal	91:98	arg1	protein					100:106	a 78 amino acid neuronal protein	75:106	a 78 amino acid neuronal protein	75:106	Neurogranin (Ng) is a 78 amino acid neuronal protein and a biomarker candidate for Alzheimer's disease (AD).
33249594	1	63	theme	neuronal	91:98	arg1	Neurogranin					55:65	Neurogranin	55:65	Neurogranin (Ng)	55:70	Neurogranin (Ng) is a 78 amino acid neuronal protein and a biomarker candidate for Alzheimer's disease (AD).
33249594	10	64	theme	several	1454:1460	arg1	forms					1472:1476	several molecular forms	1454:1476	several molecular forms of Ng	1454:1482	Taken together, this study highlights the presence of several molecular forms of Ng in CSF, comprising monomeric full-length Ng, and N- and C-terminal truncations of Ng, as well as larger forms of still unknown composition.
33249594	5	65	from	present	648:654	arg1	CSF					659:661	CSF	659:661	CSF	659:661	To obtain an overview of the different molecular forms of Ng present in CSF, we show by size exclusion chromatography (SEC), immunoblotting, immunoprecipitation, and MS that Ng is present in CSF as several molecular forms.
33249594	8	66	theme	C-terminal	1137:1146	arg1	ratios					1166:1171	the overall C-terminal fragment/total-Ng ratios	1125:1171	the overall C-terminal fragment/total-Ng ratios	1125:1171	There were no differences in the overall C-terminal fragment/total-Ng ratios between samples from AD and control groups.
33249594	5	67	theme	several	785:791	arg1	forms					803:807	several molecular forms	785:807	several molecular forms	785:807	To obtain an overview of the different molecular forms of Ng present in CSF, we show by size exclusion chromatography (SEC), immunoblotting, immunoprecipitation, and MS that Ng is present in CSF as several molecular forms.
33249594	5	68	from	forms	636:640	arg1	CSF					659:661	CSF	659:661	CSF	659:661	To obtain an overview of the different molecular forms of Ng present in CSF, we show by size exclusion chromatography (SEC), immunoblotting, immunoprecipitation, and MS that Ng is present in CSF as several molecular forms.
33249594	8	69	from	differences	1110:1120	arg1	ratios					1166:1171	the overall C-terminal fragment/total-Ng ratios	1125:1171	the overall C-terminal fragment/total-Ng ratios	1125:1171	There were no differences in the overall C-terminal fragment/total-Ng ratios between samples from AD and control groups.
33249594	8	70	theme	overall	1129:1135	arg1	ratios					1166:1171	the overall C-terminal fragment/total-Ng ratios	1125:1171	the overall C-terminal fragment/total-Ng ratios	1125:1171	There were no differences in the overall C-terminal fragment/total-Ng ratios between samples from AD and control groups.
33249594	5	71	from	CSF	778:780	arg1	present					767:773	present	767:773	present	767:773	To obtain an overview of the different molecular forms of Ng present in CSF, we show by size exclusion chromatography (SEC), immunoblotting, immunoprecipitation, and MS that Ng is present in CSF as several molecular forms.
33249594	6	72	theme	weight	866:871	arg1	forms					873:877	higher molecular weight forms	849:877	higher molecular weight forms of Ng	849:883	Besides monomeric full-length Ng, also higher molecular weight forms of Ng, and C-terminal- and previously not identified N-terminal fragments were observed.
33249594	4	73	from	present	511:517	arg1	CSF					522:524	CSF	522:524	CSF	522:524	Up to now, Ng has been shown to be present in CSF as both C-terminal fragments as well as full-length protein.
33249594	7	74	from	signal	1073:1078	arg1	samples					1087:1093	CSF samples	1083:1093	CSF samples	1083:1093	We found by immunodepletion that C-terminal peptides contribute on average to ~50% of the total-Ng ELISA signal in CSF samples.
33249594	4	75	theme	full-length	566:576	arg1	protein					578:584	full-length protein	566:584	both C-terminal fragments as well as full-length protein	529:584	Up to now, Ng has been shown to be present in CSF as both C-terminal fragments as well as full-length protein.
33249594	6	76	theme	molecular	856:864	arg1	forms					873:877	higher molecular weight forms	849:877	higher molecular weight forms of Ng	849:883	Besides monomeric full-length Ng, also higher molecular weight forms of Ng, and C-terminal- and previously not identified N-terminal fragments were observed.
33249594	7	77	theme	signal	1073:1078	arg1	%					1049:1049	~50%	1046:1049	~50% of the total-Ng ELISA signal in CSF samples	1046:1093	We found by immunodepletion that C-terminal peptides contribute on average to ~50% of the total-Ng ELISA signal in CSF samples.
33249594	7	77	theme	signal	1073:1078	arg1	signal					1073:1078	the total-Ng ELISA signal	1054:1078	the total-Ng ELISA signal in CSF samples	1054:1093	We found by immunodepletion that C-terminal peptides contribute on average to ~50% of the total-Ng ELISA signal in CSF samples.
33249594	0	78	from	neurogranin	19:29	arg1	fluid					48:52	cerebrospinal fluid	34:52	cerebrospinal fluid	34:52	Molecular forms of neurogranin in cerebrospinal fluid.
33249594	10	79	theme	composition	1611:1621	arg1	Ng					1525:1526	monomeric full-length Ng	1503:1526	monomeric full-length Ng	1503:1526	Taken together, this study highlights the presence of several molecular forms of Ng in CSF, comprising monomeric full-length Ng, and N- and C-terminal truncations of Ng, as well as larger forms of still unknown composition.
33249594	10	79	theme	composition	1611:1621	arg1	N-					1533:1534	N-	1533:1534	N-	1533:1534	Taken together, this study highlights the presence of several molecular forms of Ng in CSF, comprising monomeric full-length Ng, and N- and C-terminal truncations of Ng, as well as larger forms of still unknown composition.
33249594	10	79	theme	composition	1611:1621	arg1	forms					1588:1592	larger forms	1581:1592	larger forms of still unknown composition	1581:1621	Taken together, this study highlights the presence of several molecular forms of Ng in CSF, comprising monomeric full-length Ng, and N- and C-terminal truncations of Ng, as well as larger forms of still unknown composition.
33249594	10	79	theme	composition	1611:1621	arg1	truncations					1551:1561	C-terminal truncations	1540:1561	C-terminal truncations	1540:1561	Taken together, this study highlights the presence of several molecular forms of Ng in CSF, comprising monomeric full-length Ng, and N- and C-terminal truncations of Ng, as well as larger forms of still unknown composition.
33249594	1	80	theme	biomarker	114:122	arg1	candidate					124:132	a biomarker candidate	112:132	a biomarker candidate for Alzheimer's disease (AD)	112:161	Neurogranin (Ng) is a 78 amino acid neuronal protein and a biomarker candidate for Alzheimer's disease (AD).
33249594	6	81	theme	higher	849:854	arg1	forms					873:877	higher molecular weight forms	849:877	higher molecular weight forms of Ng	849:883	Besides monomeric full-length Ng, also higher molecular weight forms of Ng, and C-terminal- and previously not identified N-terminal fragments were observed.
33441935	0	0	theme	fibers	95:100	arg1	structure					43:51	structure	43:51	structure	43:51	Hydrogen peroxide modification affects the structure and physicochemical properties of dietary fibers from white turnip (Brassica Rapa L.).
33441935	0	0	theme	fibers	95:100	arg1	properties					73:82	physicochemical properties	57:82	physicochemical properties	57:82	Hydrogen peroxide modification affects the structure and physicochemical properties of dietary fibers from white turnip (Brassica Rapa L.).
33441935	2	1	theme	modified	429:436	arg1	IDF					438:440	modified IDF	429:440	modified IDF (MIDF)	429:447	Soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) were obtained from white turnips, and the IDF was modified with alkaline hydrogen peroxide to obtain modified IDF (MIDF) and modified SDF (MSDF).
33441935	2	1	theme	modified	429:436	arg1	MIDF					443:446	MIDF	443:446	MIDF	443:446	Soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) were obtained from white turnips, and the IDF was modified with alkaline hydrogen peroxide to obtain modified IDF (MIDF) and modified SDF (MSDF).
33441935	4	2	theme	scanning	760:767	arg1	SEM					790:792	SEM	790:792	SEM	790:792	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	4	2	theme	scanning	760:767	arg1	microscopy					778:787	scanning electron microscopy	760:787	scanning electron microscopy (SEM)	760:793	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	8	3	theme	oil	1477:1479	arg1	OAC					1502:1504	OAC	1502:1504	OAC	1502:1504	The water holding capacity (WHC), oil adsorption capacity (OAC) and water swelling capacity (WSC) showed positive correlations with each other.
33441935	8	3	theme	oil	1477:1479	arg1	capacity					1492:1499	oil adsorption capacity	1477:1499	oil adsorption capacity (OAC)	1477:1505	The water holding capacity (WHC), oil adsorption capacity (OAC) and water swelling capacity (WSC) showed positive correlations with each other.
33441935	7	4	theme	particle	1378:1385	arg1	size					1387:1390	the particle size	1374:1390	the particle size	1374:1390	Pearson correlation analysis showed that the particle size was positively correlated with the pectin content.
33441935	0	5	theme	dietary	87:93	arg1	fibers					95:100	dietary fibers	87:100	dietary fibers from white turnip (Brassica Rapa L.)	87:137	Hydrogen peroxide modification affects the structure and physicochemical properties of dietary fibers from white turnip (Brassica Rapa L.).
33441935	7	6	theme	correlation	1341:1351	arg1	analysis					1353:1360	Pearson correlation analysis	1333:1360	Pearson correlation analysis	1333:1360	Pearson correlation analysis showed that the particle size was positively correlated with the pectin content.
33441935	6	7	theme	MDFs	1273:1276	arg1	properties					1255:1264	the physical and functional properties	1227:1264	the physical and functional properties of the MDFs	1227:1276	Therefore, due to structural changes, the physical and functional properties of the MDFs were improved compared to those of the unmodified DFs.
33441935	9	8	theme	new	1646:1648	arg1	turnip					1618:1623	white turnip	1612:1623	white turnip	1612:1623	This work indicated that white turnip could be a potential new source of DFs, which presented desirable functional properties after modification.
33441935	9	8	theme	new	1646:1648	arg1	source					1650:1655	a potential new source	1634:1655	a potential new source	1634:1655	This work indicated that white turnip could be a potential new source of DFs, which presented desirable functional properties after modification.
33441935	0	9	theme	white	107:111	arg1	L					135:135	Brassica Rapa L.	121:136	Brassica Rapa L.	121:136	Hydrogen peroxide modification affects the structure and physicochemical properties of dietary fibers from white turnip (Brassica Rapa L.).
33441935	0	9	theme	white	107:111	arg1	turnip					113:118	white turnip	107:118	white turnip (Brassica Rapa L.)	107:137	Hydrogen peroxide modification affects the structure and physicochemical properties of dietary fibers from white turnip (Brassica Rapa L.).
33441935	4	10	theme	microscopy	778:787	arg1	results					749:755	The results	745:755	The results of scanning electron microscopy (SEM)	745:793	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	3	11	theme	structural	493:502	arg1	properties					520:529	The compositional, structural, and functional properties	474:529	The compositional, structural, and functional properties of the four samples	474:549	The compositional, structural, and functional properties of the four samples were investigated.
33441935	2	12	theme	Soluble	266:272	arg1	fiber					282:286	Soluble dietary fiber	266:286	Soluble dietary fiber (SDF)	266:292	Soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) were obtained from white turnips, and the IDF was modified with alkaline hydrogen peroxide to obtain modified IDF (MIDF) and modified SDF (MSDF).
33441935	2	12	theme	Soluble	266:272	arg1	SDF					289:291	SDF	289:291	SDF	289:291	Soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) were obtained from white turnips, and the IDF was modified with alkaline hydrogen peroxide to obtain modified IDF (MIDF) and modified SDF (MSDF).
33441935	1	13	theme	high	238:241	arg1	content					257:263	high dietary fiber content	238:263	high dietary fiber content	238:263	Turnip (Brassica rapa L.) is widely consumed as a vegetable and traditional Chinese medicine with high dietary fiber content.
33441935	4	14	theme	FT-IR	826:830	arg1	spectroscopy					833:844	infrared (FT-IR) spectroscopy	816:844	infrared (FT-IR) spectroscopy	816:844	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	4	15	theme	electron	769:776	arg1	SEM					790:792	SEM	790:792	SEM	790:792	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	4	15	theme	electron	769:776	arg1	microscopy					778:787	scanning electron microscopy	760:787	scanning electron microscopy (SEM)	760:793	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	8	16	theme	holding	1453:1459	arg1	WHC					1471:1473	WHC	1471:1473	WHC	1471:1473	The water holding capacity (WHC), oil adsorption capacity (OAC) and water swelling capacity (WSC) showed positive correlations with each other.
33441935	8	16	theme	holding	1453:1459	arg1	capacity					1461:1468	The water holding capacity	1443:1468	The water holding capacity (WHC)	1443:1474	The water holding capacity (WHC), oil adsorption capacity (OAC) and water swelling capacity (WSC) showed positive correlations with each other.
33441935	1	17	theme	dietary	243:249	arg1	content					257:263	high dietary fiber content	238:263	high dietary fiber content	238:263	Turnip (Brassica rapa L.) is widely consumed as a vegetable and traditional Chinese medicine with high dietary fiber content.
33441935	9	18	theme	functional	1691:1700	arg1	properties					1702:1711	desirable functional properties	1681:1711	desirable functional properties	1681:1711	This work indicated that white turnip could be a potential new source of DFs, which presented desirable functional properties after modification.
33441935	5	19	contain	had	1072:1074	arg1	MDFs					1067:1070	the MDFs	1063:1070	the MDFs	1063:1070	Analysis of the microrheological behaviors showed that the MDFs had higher viscosity than that of the DFs, with a looser structure for the MSDF and a stable structure for the MIDF.
33441935	5	19	contain	had	1072:1074	arg2	viscosity					1083:1091	higher viscosity	1076:1091	higher viscosity than that of the DFs	1076:1112	Analysis of the microrheological behaviors showed that the MDFs had higher viscosity than that of the DFs, with a looser structure for the MSDF and a stable structure for the MIDF.
33441935	9	20	theme	DFs	1660:1662	arg1	turnip					1618:1623	white turnip	1612:1623	white turnip	1612:1623	This work indicated that white turnip could be a potential new source of DFs, which presented desirable functional properties after modification.
33441935	9	20	theme	DFs	1660:1662	arg1	source					1650:1655	a potential new source	1634:1655	a potential new source	1634:1655	This work indicated that white turnip could be a potential new source of DFs, which presented desirable functional properties after modification.
33441935	5	21	theme	stable	1158:1163	arg1	structure					1165:1173	a stable structure	1156:1173	a stable structure for the MIDF	1156:1186	Analysis of the microrheological behaviors showed that the MDFs had higher viscosity than that of the DFs, with a looser structure for the MSDF and a stable structure for the MIDF.
33441935	1	22	theme	Brassica	148:155	arg1	L.					162:163	Brassica rapa L.	148:163	Brassica rapa L.	148:163	Turnip (Brassica rapa L.) is widely consumed as a vegetable and traditional Chinese medicine with high dietary fiber content.
33441935	1	22	theme	Brassica	148:155	arg1	Turnip					140:145	Turnip	140:145	Turnip (Brassica rapa L.)	140:164	Turnip (Brassica rapa L.) is widely consumed as a vegetable and traditional Chinese medicine with high dietary fiber content.
33441935	4	23	theme	differential	875:886	arg1	DSC					910:912	DSC	910:912	DSC	910:912	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	4	23	theme	differential	875:886	arg1	calorimetry					897:907	differential scanning calorimetry	875:907	differential scanning calorimetry (DSC)	875:913	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	0	24	from	structure	43:51	arg1	L					135:135	Brassica Rapa L.	121:136	Brassica Rapa L.	121:136	Hydrogen peroxide modification affects the structure and physicochemical properties of dietary fibers from white turnip (Brassica Rapa L.).
33441935	0	24	from	structure	43:51	arg1	turnip					113:118	white turnip	107:118	white turnip (Brassica Rapa L.)	107:137	Hydrogen peroxide modification affects the structure and physicochemical properties of dietary fibers from white turnip (Brassica Rapa L.).
33441935	8	25	theme	water	1447:1451	arg1	WHC					1471:1473	WHC	1471:1473	WHC	1471:1473	The water holding capacity (WHC), oil adsorption capacity (OAC) and water swelling capacity (WSC) showed positive correlations with each other.
33441935	8	25	theme	water	1447:1451	arg1	capacity					1461:1468	The water holding capacity	1443:1468	The water holding capacity (WHC)	1443:1474	The water holding capacity (WHC), oil adsorption capacity (OAC) and water swelling capacity (WSC) showed positive correlations with each other.
33441935	4	26	theme	pectin	677:682	arg1	sizes					649:653	smaller particle sizes	632:653	smaller particle sizes	632:653	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	4	26	theme	pectin	677:682	arg1	contents					665:672	lower contents	659:672	lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs)	659:743	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	4	27	theme	hydroxyl	991:998	arg1	groups					1000:1005	more exposed hydroxyl groups	978:1005	more exposed hydroxyl groups	978:1005	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	1	28	theme	rapa	157:160	arg1	L.					162:163	Brassica rapa L.	148:163	Brassica rapa L.	148:163	Turnip (Brassica rapa L.) is widely consumed as a vegetable and traditional Chinese medicine with high dietary fiber content.
33441935	1	28	theme	rapa	157:160	arg1	Turnip					140:145	Turnip	140:145	Turnip (Brassica rapa L.)	140:164	Turnip (Brassica rapa L.) is widely consumed as a vegetable and traditional Chinese medicine with high dietary fiber content.
33441935	0	29	theme	peroxide	9:16	arg1	modification					18:29	Hydrogen peroxide modification	0:29	Hydrogen peroxide modification	0:29	Hydrogen peroxide modification affects the structure and physicochemical properties of dietary fibers from white turnip (Brassica Rapa L.).
33441935	5	30	theme	behaviors	1041:1049	arg1	Analysis					1008:1015	Analysis	1008:1015	Analysis of the microrheological behaviors	1008:1049	Analysis of the microrheological behaviors showed that the MDFs had higher viscosity than that of the DFs, with a looser structure for the MSDF and a stable structure for the MIDF.
33441935	3	31	theme	compositional	478:490	arg1	properties					520:529	The compositional, structural, and functional properties	474:529	The compositional, structural, and functional properties of the four samples	474:549	The compositional, structural, and functional properties of the four samples were investigated.
33441935	4	32	theme	exposed	983:989	arg1	groups					1000:1005	more exposed hydroxyl groups	978:1005	more exposed hydroxyl groups	978:1005	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	0	33	theme	Hydrogen	0:7	arg1	peroxide					9:16	Hydrogen peroxide	0:16	Hydrogen peroxide modification	0:29	Hydrogen peroxide modification affects the structure and physicochemical properties of dietary fibers from white turnip (Brassica Rapa L.).
33441935	1	34	theme	fiber	251:255	arg1	content					257:263	high dietary fiber content	238:263	high dietary fiber content	238:263	Turnip (Brassica rapa L.) is widely consumed as a vegetable and traditional Chinese medicine with high dietary fiber content.
33441935	4	35	dep	sizes	649:653	arg1	results					749:755	The results	745:755	The results of scanning electron microscopy (SEM)	745:793	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	4	36	theme	scanning	888:895	arg1	DSC					910:912	DSC	910:912	DSC	910:912	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	4	36	theme	scanning	888:895	arg1	calorimetry					897:907	differential scanning calorimetry	875:907	differential scanning calorimetry (DSC)	875:913	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	4	37	theme	particle	640:647	arg1	sizes					649:653	smaller particle sizes	632:653	smaller particle sizes	632:653	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	9	38	theme	white	1612:1616	arg1	turnip					1618:1623	white turnip	1612:1623	white turnip	1612:1623	This work indicated that white turnip could be a potential new source of DFs, which presented desirable functional properties after modification.
33441935	9	38	theme	white	1612:1616	arg1	source					1650:1655	a potential new source	1634:1655	a potential new source	1634:1655	This work indicated that white turnip could be a potential new source of DFs, which presented desirable functional properties after modification.
33441935	6	39	theme	functional	1244:1253	arg1	properties					1255:1264	the physical and functional properties	1227:1264	the physical and functional properties of the MDFs	1227:1276	Therefore, due to structural changes, the physical and functional properties of the MDFs were improved compared to those of the unmodified DFs.
33441935	5	40	theme	microrheological	1024:1039	arg1	behaviors					1041:1049	the microrheological behaviors	1020:1049	the microrheological behaviors	1020:1049	Analysis of the microrheological behaviors showed that the MDFs had higher viscosity than that of the DFs, with a looser structure for the MSDF and a stable structure for the MIDF.
33441935	0	41	theme	Rapa	130:133	arg1	L					135:135	Brassica Rapa L.	121:136	Brassica Rapa L.	121:136	Hydrogen peroxide modification affects the structure and physicochemical properties of dietary fibers from white turnip (Brassica Rapa L.).
33441935	0	41	theme	Rapa	130:133	arg1	turnip					113:118	white turnip	107:118	white turnip (Brassica Rapa L.)	107:137	Hydrogen peroxide modification affects the structure and physicochemical properties of dietary fibers from white turnip (Brassica Rapa L.).
33441935	4	42	theme	lower	659:663	arg1	contents					665:672	lower contents	659:672	lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs)	659:743	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	8	43	theme	swelling	1517:1524	arg1	capacity					1526:1533	water swelling capacity	1511:1533	water swelling capacity (WSC)	1511:1539	The water holding capacity (WHC), oil adsorption capacity (OAC) and water swelling capacity (WSC) showed positive correlations with each other.
33441935	8	43	theme	swelling	1517:1524	arg1	WSC					1536:1538	WSC	1536:1538	WSC	1536:1538	The water holding capacity (WHC), oil adsorption capacity (OAC) and water swelling capacity (WSC) showed positive correlations with each other.
33441935	2	44	mod	modified	378:385	arg1	IDF					370:372	the IDF	366:372	the IDF	366:372	Soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) were obtained from white turnips, and the IDF was modified with alkaline hydrogen peroxide to obtain modified IDF (MIDF) and modified SDF (MSDF).
33441935	2	44	mod	modified	378:385	arg3	peroxide					410:417	alkaline hydrogen peroxide	392:417	alkaline hydrogen peroxide to obtain modified IDF (MIDF) and modified SDF (MSDF)	392:471	Soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) were obtained from white turnips, and the IDF was modified with alkaline hydrogen peroxide to obtain modified IDF (MIDF) and modified SDF (MSDF).
33441935	0	45	theme	Brassica	121:128	arg1	L					135:135	Brassica Rapa L.	121:136	Brassica Rapa L.	121:136	Hydrogen peroxide modification affects the structure and physicochemical properties of dietary fibers from white turnip (Brassica Rapa L.).
33441935	0	45	theme	Brassica	121:128	arg1	turnip					113:118	white turnip	107:118	white turnip (Brassica Rapa L.)	107:137	Hydrogen peroxide modification affects the structure and physicochemical properties of dietary fibers from white turnip (Brassica Rapa L.).
33441935	2	46	theme	dietary	274:280	arg1	fiber					282:286	Soluble dietary fiber	266:286	Soluble dietary fiber (SDF)	266:292	Soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) were obtained from white turnips, and the IDF was modified with alkaline hydrogen peroxide to obtain modified IDF (MIDF) and modified SDF (MSDF).
33441935	2	46	theme	dietary	274:280	arg1	SDF					289:291	SDF	289:291	SDF	289:291	Soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) were obtained from white turnips, and the IDF was modified with alkaline hydrogen peroxide to obtain modified IDF (MIDF) and modified SDF (MSDF).
33441935	6	47	theme	unmodified	1317:1326	arg1	DFs					1328:1330	the unmodified DFs	1313:1330	the unmodified DFs	1313:1330	Therefore, due to structural changes, the physical and functional properties of the MDFs were improved compared to those of the unmodified DFs.
33441935	9	48	theme	desirable	1681:1689	arg1	properties					1702:1711	desirable functional properties	1681:1711	desirable functional properties	1681:1711	This work indicated that white turnip could be a potential new source of DFs, which presented desirable functional properties after modification.
33441935	6	49	theme	physical	1231:1238	arg1	properties					1255:1264	the physical and functional properties	1227:1264	the physical and functional properties of the MDFs	1227:1276	Therefore, due to structural changes, the physical and functional properties of the MDFs were improved compared to those of the unmodified DFs.
33441935	8	50	theme	water	1511:1515	arg1	capacity					1526:1533	water swelling capacity	1511:1533	water swelling capacity (WSC)	1511:1539	The water holding capacity (WHC), oil adsorption capacity (OAC) and water swelling capacity (WSC) showed positive correlations with each other.
33441935	8	50	theme	water	1511:1515	arg1	WSC					1536:1538	WSC	1536:1538	WSC	1536:1538	The water holding capacity (WHC), oil adsorption capacity (OAC) and water swelling capacity (WSC) showed positive correlations with each other.
33441935	4	51	theme	dietary	724:730	arg1	DFs					740:742	DFs	740:742	DFs	740:742	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	4	51	theme	dietary	724:730	arg1	fibers					732:737	unmodified dietary fibers	713:737	unmodified dietary fibers (DFs)	713:743	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	2	52	theme	white	347:351	arg1	turnips					353:359	white turnips	347:359	white turnips	347:359	Soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) were obtained from white turnips, and the IDF was modified with alkaline hydrogen peroxide to obtain modified IDF (MIDF) and modified SDF (MSDF).
33441935	4	53	theme	infrared	816:823	arg1	spectroscopy					833:844	infrared (FT-IR) spectroscopy	816:844	infrared (FT-IR) spectroscopy	816:844	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	3	54	theme	samples	543:549	arg1	properties					520:529	The compositional, structural, and functional properties	474:529	The compositional, structural, and functional properties of the four samples	474:549	The compositional, structural, and functional properties of the four samples were investigated.
33441935	3	55	theme	functional	509:518	arg1	properties					520:529	The compositional, structural, and functional properties	474:529	The compositional, structural, and functional properties of the four samples	474:549	The compositional, structural, and functional properties of the four samples were investigated.
33441935	0	56	theme	physicochemical	57:71	arg1	properties					73:82	physicochemical properties	57:82	physicochemical properties	57:82	Hydrogen peroxide modification affects the structure and physicochemical properties of dietary fibers from white turnip (Brassica Rapa L.).
33441935	2	57	theme	modified	453:460	arg1	MSDF					467:470	MSDF	467:470	MSDF	467:470	Soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) were obtained from white turnips, and the IDF was modified with alkaline hydrogen peroxide to obtain modified IDF (MIDF) and modified SDF (MSDF).
33441935	2	57	theme	modified	453:460	arg1	SDF					462:464	modified SDF	453:464	modified SDF (MSDF)	453:471	Soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) were obtained from white turnips, and the IDF was modified with alkaline hydrogen peroxide to obtain modified IDF (MIDF) and modified SDF (MSDF).
33441935	0	58	from	properties	73:82	arg1	L					135:135	Brassica Rapa L.	121:136	Brassica Rapa L.	121:136	Hydrogen peroxide modification affects the structure and physicochemical properties of dietary fibers from white turnip (Brassica Rapa L.).
33441935	0	58	from	properties	73:82	arg1	turnip					113:118	white turnip	107:118	white turnip (Brassica Rapa L.)	107:137	Hydrogen peroxide modification affects the structure and physicochemical properties of dietary fibers from white turnip (Brassica Rapa L.).
33441935	0	59	dep	structure	43:51	arg1	the					39:41	the	39:41	the	39:41	Hydrogen peroxide modification affects the structure and physicochemical properties of dietary fibers from white turnip (Brassica Rapa L.).
33441935	2	60	theme	dietary	308:314	arg1	IDF					323:325	IDF	323:325	IDF	323:325	Soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) were obtained from white turnips, and the IDF was modified with alkaline hydrogen peroxide to obtain modified IDF (MIDF) and modified SDF (MSDF).
33441935	2	60	theme	dietary	308:314	arg1	fiber					316:320	insoluble dietary fiber	298:320	insoluble dietary fiber (IDF)	298:326	Soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) were obtained from white turnips, and the IDF was modified with alkaline hydrogen peroxide to obtain modified IDF (MIDF) and modified SDF (MSDF).
33441935	2	61	theme	alkaline	392:399	arg1	peroxide					410:417	alkaline hydrogen peroxide	392:417	alkaline hydrogen peroxide to obtain modified IDF (MIDF) and modified SDF (MSDF)	392:471	Soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) were obtained from white turnips, and the IDF was modified with alkaline hydrogen peroxide to obtain modified IDF (MIDF) and modified SDF (MSDF).
33441935	4	62	theme	X-ray	847:851	arg1	diffraction					853:863	X-ray diffraction	847:863	X-ray diffraction (XRD)	847:869	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	4	62	theme	X-ray	847:851	arg1	XRD					866:868	XRD	866:868	XRD	866:868	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	1	63	with	medicine	224:231	arg1	content					257:263	high dietary fiber content	238:263	high dietary fiber content	238:263	Turnip (Brassica rapa L.) is widely consumed as a vegetable and traditional Chinese medicine with high dietary fiber content.
33441935	2	64	theme	insoluble	298:306	arg1	IDF					323:325	IDF	323:325	IDF	323:325	Soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) were obtained from white turnips, and the IDF was modified with alkaline hydrogen peroxide to obtain modified IDF (MIDF) and modified SDF (MSDF).
33441935	2	64	theme	insoluble	298:306	arg1	fiber					316:320	insoluble dietary fiber	298:320	insoluble dietary fiber (IDF)	298:326	Soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) were obtained from white turnips, and the IDF was modified with alkaline hydrogen peroxide to obtain modified IDF (MIDF) and modified SDF (MSDF).
33441935	7	65	theme	pectin	1427:1432	arg1	content					1434:1440	the pectin content	1423:1440	the pectin content	1423:1440	Pearson correlation analysis showed that the particle size was positively correlated with the pectin content.
33441935	4	66	contain	had	974:976	arg1	smaller					962:968	smaller	962:968	smaller	962:968	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	4	66	contain	had	974:976	arg2	groups					1000:1005	more exposed hydroxyl groups	978:1005	more exposed hydroxyl groups	978:1005	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	4	66	contain	had	974:976	arg1	MDFs					952:955	the MDFs	948:955	the MDFs	948:955	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	0	67	from	turnip	113:118	arg1	structure					43:51	structure	43:51	structure	43:51	Hydrogen peroxide modification affects the structure and physicochemical properties of dietary fibers from white turnip (Brassica Rapa L.).
33441935	0	67	from	turnip	113:118	arg1	properties					73:82	physicochemical properties	57:82	physicochemical properties	57:82	Hydrogen peroxide modification affects the structure and physicochemical properties of dietary fibers from white turnip (Brassica Rapa L.).
33441935	0	67	from	turnip	113:118	arg1	fibers					95:100	dietary fibers	87:100	dietary fibers from white turnip (Brassica Rapa L.)	87:137	Hydrogen peroxide modification affects the structure and physicochemical properties of dietary fibers from white turnip (Brassica Rapa L.).
33441935	4	68	theme	unmodified	713:722	arg1	DFs					740:742	DFs	740:742	DFs	740:742	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	4	68	theme	unmodified	713:722	arg1	fibers					732:737	unmodified dietary fibers	713:737	unmodified dietary fibers (DFs)	713:743	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	4	69	theme	smaller	632:638	arg1	sizes					649:653	smaller particle sizes	632:653	smaller particle sizes	632:653	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	9	70	theme	potential	1636:1644	arg1	turnip					1618:1623	white turnip	1612:1623	white turnip	1612:1623	This work indicated that white turnip could be a potential new source of DFs, which presented desirable functional properties after modification.
33441935	9	70	theme	potential	1636:1644	arg1	source					1650:1655	a potential new source	1634:1655	a potential new source	1634:1655	This work indicated that white turnip could be a potential new source of DFs, which presented desirable functional properties after modification.
33441935	5	71	theme	higher	1076:1081	arg1	viscosity					1083:1091	higher viscosity	1076:1091	higher viscosity than that of the DFs	1076:1112	Analysis of the microrheological behaviors showed that the MDFs had higher viscosity than that of the DFs, with a looser structure for the MSDF and a stable structure for the MIDF.
33441935	8	72	theme	positive	1548:1555	arg1	correlations					1557:1568	positive correlations	1548:1568	positive correlations with each other	1548:1584	The water holding capacity (WHC), oil adsorption capacity (OAC) and water swelling capacity (WSC) showed positive correlations with each other.
33441935	4	73	theme	polyphenol	688:697	arg1	sizes					649:653	smaller particle sizes	632:653	smaller particle sizes	632:653	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	4	73	theme	polyphenol	688:697	arg1	contents					665:672	lower contents	659:672	lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs)	659:743	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	5	74	theme	looser	1122:1127	arg1	structure					1129:1137	a looser structure	1120:1137	a looser structure for the MSDF	1120:1150	Analysis of the microrheological behaviors showed that the MDFs had higher viscosity than that of the DFs, with a looser structure for the MSDF and a stable structure for the MIDF.
33441935	1	75	theme	vegetable	190:198	arg1	Turnip					140:145	Turnip	140:145	Turnip (Brassica rapa L.)	140:164	Turnip (Brassica rapa L.) is widely consumed as a vegetable and traditional Chinese medicine with high dietary fiber content.
33441935	1	75	theme	vegetable	190:198	arg1	medicine					224:231	a vegetable and traditional Chinese medicine	188:231	a vegetable and traditional Chinese medicine with high dietary fiber content	188:263	Turnip (Brassica rapa L.) is widely consumed as a vegetable and traditional Chinese medicine with high dietary fiber content.
33441935	6	76	theme	structural	1207:1216	arg1	changes					1218:1224	structural changes	1207:1224	structural changes	1207:1224	Therefore, due to structural changes, the physical and functional properties of the MDFs were improved compared to those of the unmodified DFs.
33441935	4	77	theme	dietary	603:609	arg1	fibers					611:616	the modified dietary fibers	590:616	the modified dietary fibers (MDFs)	590:623	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	4	77	theme	dietary	603:609	arg1	MDFs					619:622	MDFs	619:622	MDFs	619:622	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	1	78	theme	and traditional	200:214	arg1	Turnip					140:145	Turnip	140:145	Turnip (Brassica rapa L.)	140:164	Turnip (Brassica rapa L.) is widely consumed as a vegetable and traditional Chinese medicine with high dietary fiber content.
33441935	1	78	theme	and traditional	200:214	arg1	medicine					224:231	a vegetable and traditional Chinese medicine	188:231	a vegetable and traditional Chinese medicine with high dietary fiber content	188:263	Turnip (Brassica rapa L.) is widely consumed as a vegetable and traditional Chinese medicine with high dietary fiber content.
33441935	2	79	theme	hydrogen	401:408	arg1	peroxide					410:417	alkaline hydrogen peroxide	392:417	alkaline hydrogen peroxide to obtain modified IDF (MIDF) and modified SDF (MSDF)	392:471	Soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) were obtained from white turnips, and the IDF was modified with alkaline hydrogen peroxide to obtain modified IDF (MIDF) and modified SDF (MSDF).
33441935	8	80	with	correlations	1557:1568	arg1	other					1580:1584	other	1580:1584	other	1580:1584	The water holding capacity (WHC), oil adsorption capacity (OAC) and water swelling capacity (WSC) showed positive correlations with each other.
33441935	7	81	theme	Pearson	1333:1339	arg1	analysis					1353:1360	Pearson correlation analysis	1333:1360	Pearson correlation analysis	1333:1360	Pearson correlation analysis showed that the particle size was positively correlated with the pectin content.
33441935	4	82	theme	modified	594:601	arg1	fibers					611:616	the modified dietary fibers	590:616	the modified dietary fibers (MDFs)	590:623	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	4	82	theme	modified	594:601	arg1	MDFs					619:622	MDFs	619:622	MDFs	619:622	After modification, the modified dietary fibers (MDFs) showed smaller particle sizes and lower contents of pectin and polyphenol than those of unmodified dietary fibers (DFs) The results of scanning electron microscopy (SEM), Fourier transformed infrared (FT-IR) spectroscopy, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that compared to the DFs, the MDFs were smaller and had more exposed hydroxyl groups.
33441935	8	83	theme	adsorption	1481:1490	arg1	OAC					1502:1504	OAC	1502:1504	OAC	1502:1504	The water holding capacity (WHC), oil adsorption capacity (OAC) and water swelling capacity (WSC) showed positive correlations with each other.
33441935	8	83	theme	adsorption	1481:1490	arg1	capacity					1492:1499	oil adsorption capacity	1477:1499	oil adsorption capacity (OAC)	1477:1505	The water holding capacity (WHC), oil adsorption capacity (OAC) and water swelling capacity (WSC) showed positive correlations with each other.
33441935	1	84	theme	Chinese	216:222	arg1	Turnip					140:145	Turnip	140:145	Turnip (Brassica rapa L.)	140:164	Turnip (Brassica rapa L.) is widely consumed as a vegetable and traditional Chinese medicine with high dietary fiber content.
33441935	1	84	theme	Chinese	216:222	arg1	medicine					224:231	a vegetable and traditional Chinese medicine	188:231	a vegetable and traditional Chinese medicine with high dietary fiber content	188:263	Turnip (Brassica rapa L.) is widely consumed as a vegetable and traditional Chinese medicine with high dietary fiber content.
34707140	4	0	theme	main	507:510	arg1	objective					512:520	The main objective	503:520	The main objective of this project	503:536	The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye.
34707140	11	1	theme	study	1371:1375	arg1	results					1356:1362	The results	1352:1362	The results of the study	1352:1375	The results of the study indicate a stronger response of the white strain to HMD compared to the wild-type strain.
34707140	3	2	theme	other	466:470	arg1	molecules					492:500	other biologically active molecules	466:500	other biologically active molecules	466:500	It has been shown to have a number of essential functions, such as in the production of other biologically active molecules.
34707140	4	3	theme	high	574:577	arg1	HMD					600:602	HMD	600:602	HMD	600:602	The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye.
34707140	4	3	theme	high	574:577	arg1	diet					594:597	a high monosaccharide diet	572:597	a high monosaccharide diet (HMD)	572:603	The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye.
34707140	4	4	theme	diet	594:597	arg1	effects					561:567	the effects	557:567	the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye	557:807	The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye.
34707140	4	5	with	strain	678:683	arg1	synthesis					714:722	impaired visual pigment synthesis	690:722	impaired visual pigment synthesis	690:722	The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye.
34707140	0	6	theme	cricket	121:127	arg1	domesticus					137:146	house cricket (Acheta domesticus)	115:147	house cricket (Acheta domesticus)	115:147	The effects of high-monosaccharide diets on development and biochemical composition of white-eyed mutant strain of house cricket (Acheta domesticus).
34707140	3	7	theme	active	485:490	arg1	molecules					492:500	other biologically active molecules	466:500	other biologically active molecules	466:500	It has been shown to have a number of essential functions, such as in the production of other biologically active molecules.
34707140	0	8	theme	Acheta	130:135	arg1	domesticus					137:146	house cricket (Acheta domesticus)	115:147	house cricket (Acheta domesticus)	115:147	The effects of high-monosaccharide diets on development and biochemical composition of white-eyed mutant strain of house cricket (Acheta domesticus).
34707140	1	9	theme	amino	191:195	arg1	acids					197:201	the essential amino acids	177:201	the essential amino acids in the animal body	177:220	Tryptophan (TRP) is one of the essential amino acids in the animal body.
34707140	3	10	contain	have	399:402	arg2	number					406:411	a number	404:411	a number of essential functions	404:434	It has been shown to have a number of essential functions, such as in the production of other biologically active molecules.
34707140	3	10	contain	have	399:402	arg1	It					378:379	It	378:379	It	378:379	It has been shown to have a number of essential functions, such as in the production of other biologically active molecules.
34707140	4	11	theme	hemimetabolic	610:622	arg1	domesticus					653:662	Acheta domesticus	646:662	Acheta domesticus	646:662	The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye.
34707140	4	11	theme	hemimetabolic	610:622	arg1	cricket					637:643	a hemimetabolic insect-house cricket	608:643	a hemimetabolic insect-house cricket (Acheta domesticus)	608:663	The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye.
34707140	4	12	theme	-white	798:803	arg1	eye					805:807	-white eye	798:807	-white eye	798:807	The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye.
34707140	8	13	theme	glycogen	1194:1201	arg1	content					1203:1209	glycogen content	1194:1209	glycogen content	1194:1209	Biochemical analyses demonstrated the effects of HMD mainly on fat and glycogen content.
34707140	7	14	theme	KYN/TRP	1108:1114	arg1	ratio					1116:1120	an elevated KYN/TRP ratio	1096:1120	an elevated KYN/TRP ratio	1096:1120	ELISA assays indicated dysfunction of the TRP-KYN pathway in white strain insects and an elevated KYN/TRP ratio.
34707140	10	15	theme	imago	1301:1305	arg1	weight					1312:1317	imago body weight	1301:1317	imago body weight	1301:1317	However, no changes in imago body weight and water content were observed.
34707140	12	16	theme	fructose	1518:1525	arg1	apparent					1547:1554	apparent	1547:1554	apparent	1547:1554	At the same time, a stronger detrimental effect of fructose than of glucose was apparent.
34707140	12	16	theme	fructose	1518:1525	arg1	effect					1508:1513	a stronger detrimental effect	1485:1513	a stronger detrimental effect of fructose than of glucose	1485:1541	At the same time, a stronger detrimental effect of fructose than of glucose was apparent.
34707140	7	17	from	dysfunction	1033:1043	arg1	ratio					1116:1120	an elevated KYN/TRP ratio	1096:1120	an elevated KYN/TRP ratio	1096:1120	ELISA assays indicated dysfunction of the TRP-KYN pathway in white strain insects and an elevated KYN/TRP ratio.
34707140	7	17	from	dysfunction	1033:1043	arg1	insects					1084:1090	white strain insects	1071:1090	white strain insects	1071:1090	ELISA assays indicated dysfunction of the TRP-KYN pathway in white strain insects and an elevated KYN/TRP ratio.
34707140	0	18	theme	mutant	98:103	arg1	strain					105:110	white-eyed mutant strain	87:110	white-eyed mutant strain of house cricket (Acheta domesticus)	87:147	The effects of high-monosaccharide diets on development and biochemical composition of white-eyed mutant strain of house cricket (Acheta domesticus).
34707140	10	19	theme	water	1323:1327	arg1	content					1329:1335	water content	1323:1335	water content	1323:1335	However, no changes in imago body weight and water content were observed.
34707140	7	20	theme	white	1071:1075	arg1	insects					1084:1090	white strain insects	1071:1090	white strain insects	1071:1090	ELISA assays indicated dysfunction of the TRP-KYN pathway in white strain insects and an elevated KYN/TRP ratio.
34707140	5	21	theme	glucose	861:867	arg1	effects					850:856	the effects	846:856	the effects of glucose and fructose on cricket development and biochemical composition	846:931	This study was aimed at determining the effects of glucose and fructose on cricket development and biochemical composition.
34707140	12	22	theme	stronger	1487:1494	arg1	apparent					1547:1554	apparent	1547:1554	apparent	1547:1554	At the same time, a stronger detrimental effect of fructose than of glucose was apparent.
34707140	12	22	theme	stronger	1487:1494	arg1	effect					1508:1513	a stronger detrimental effect	1485:1513	a stronger detrimental effect of fructose than of glucose	1485:1541	At the same time, a stronger detrimental effect of fructose than of glucose was apparent.
34707140	4	23	theme	Acheta	646:651	arg1	domesticus					653:662	Acheta domesticus	646:662	Acheta domesticus	646:662	The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye.
34707140	4	23	theme	Acheta	646:651	arg1	cricket					637:643	a hemimetabolic insect-house cricket	608:643	a hemimetabolic insect-house cricket (Acheta domesticus)	608:663	The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye.
34707140	7	24	theme	pathway	1060:1066	arg1	dysfunction					1033:1043	dysfunction	1033:1043	dysfunction of the TRP-KYN pathway in white strain insects and an elevated KYN/TRP ratio	1033:1120	ELISA assays indicated dysfunction of the TRP-KYN pathway in white strain insects and an elevated KYN/TRP ratio.
34707140	9	25	from	decrease	1214:1221	arg1	intake					1231:1236	food intake	1226:1236	food intake	1226:1236	A decrease in food intake was also observed in the groups on HMD.
34707140	12	26	theme	same	1474:1477	arg1	time					1479:1482	the same time	1470:1482	the same time	1470:1482	At the same time, a stronger detrimental effect of fructose than of glucose was apparent.
34707140	6	27	theme	parallel	936:943	arg1	goal					945:948	A parallel goal	934:948	A parallel goal	934:948	A parallel goal was to compare the response of both cricket strains to HMD.
34707140	0	28	theme	diets	35:39	arg1	effects					4:10	The effects	0:10	The effects of high-monosaccharide diets on development and biochemical composition of white-eyed mutant strain of house cricket (Acheta domesticus).	0:148	The effects of high-monosaccharide diets on development and biochemical composition of white-eyed mutant strain of house cricket (Acheta domesticus).
34707140	0	29	theme	biochemical	60:70	arg1	composition					72:82	biochemical composition	60:82	biochemical composition	60:82	The effects of high-monosaccharide diets on development and biochemical composition of white-eyed mutant strain of house cricket (Acheta domesticus).
34707140	4	30	theme	related	733:739	arg1	strain					678:683	a mutant strain	669:683	a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)	669:797	The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye.
34707140	3	31	theme	essential	416:424	arg1	functions					426:434	essential functions	416:434	essential functions	416:434	It has been shown to have a number of essential functions, such as in the production of other biologically active molecules.
34707140	4	32	theme	impaired	690:697	arg1	synthesis					714:722	impaired visual pigment synthesis	690:722	impaired visual pigment synthesis	690:722	The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye.
34707140	1	33	theme	acids	197:201	arg1	acids					197:201	the essential amino acids	177:201	the essential amino acids in the animal body	177:220	Tryptophan (TRP) is one of the essential amino acids in the animal body.
34707140	1	33	theme	acids	197:201	arg1	one					170:172	one	170:172	one	170:172	Tryptophan (TRP) is one of the essential amino acids in the animal body.
34707140	5	34	theme	fructose	873:880	arg1	effects					850:856	the effects	846:856	the effects of glucose and fructose on cricket development and biochemical composition	846:931	This study was aimed at determining the effects of glucose and fructose on cricket development and biochemical composition.
34707140	6	35	theme	strains	994:1000	arg1	response					969:976	the response	965:976	the response of both cricket strains to HMD	965:1007	A parallel goal was to compare the response of both cricket strains to HMD.
34707140	4	36	theme	pigment	706:712	arg1	synthesis					714:722	impaired visual pigment synthesis	690:722	impaired visual pigment synthesis	690:722	The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye.
34707140	2	37	theme	molecules	320:328	arg1	one					294:296	one	294:296	one	294:296	Its exogenicity and low concentrations mean that it can be regarded as one of the key regulatory molecules at the cellular as well as physiological level.
34707140	2	37	theme	molecules	320:328	arg1	it					272:273	it	272:273	it	272:273	Its exogenicity and low concentrations mean that it can be regarded as one of the key regulatory molecules at the cellular as well as physiological level.
34707140	2	37	theme	molecules	320:328	arg1	molecules					320:328	the key regulatory molecules	301:328	the key regulatory molecules at the cellular as well as physiological level	301:375	Its exogenicity and low concentrations mean that it can be regarded as one of the key regulatory molecules at the cellular as well as physiological level.
34707140	5	38	theme	cricket	885:891	arg1	development					893:903	cricket development	885:903	cricket development	885:903	This study was aimed at determining the effects of glucose and fructose on cricket development and biochemical composition.
34707140	2	39	from	level	371:375	arg1	molecules					320:328	the key regulatory molecules	301:328	the key regulatory molecules at the cellular as well as physiological level	301:375	Its exogenicity and low concentrations mean that it can be regarded as one of the key regulatory molecules at the cellular as well as physiological level.
34707140	2	40	theme	key	305:307	arg1	molecules					320:328	the key regulatory molecules	301:328	the key regulatory molecules at the cellular as well as physiological level	301:375	Its exogenicity and low concentrations mean that it can be regarded as one of the key regulatory molecules at the cellular as well as physiological level.
34707140	4	41	theme	kynurenine	763:772	arg1	KYN					775:777	KYN	775:777	KYN	775:777	The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye.
34707140	4	41	theme	kynurenine	763:772	arg1	pathway					790:796	the tryptophan and kynurenine (KYN) metabolic pathway	744:796	pathway	790:796	The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye.
34707140	13	42	theme	modulating	1579:1588	arg1	Sex					1557:1559	Sex	1557:1559	Sex	1557:1559	Sex was found to be a modulating factor in the response to HMD.
34707140	13	42	theme	modulating	1579:1588	arg1	factor					1590:1595	a modulating factor	1577:1595	a modulating factor	1577:1595	Sex was found to be a modulating factor in the response to HMD.
34707140	11	43	theme	stronger	1388:1395	arg1	response					1397:1404	a stronger response	1386:1404	a stronger response of the white strain to HMD compared to the wild-type strain	1386:1464	The results of the study indicate a stronger response of the white strain to HMD compared to the wild-type strain.
34707140	5	44	from	effects	850:856	arg1	development					893:903	cricket development	885:903	cricket development	885:903	This study was aimed at determining the effects of glucose and fructose on cricket development and biochemical composition.
34707140	5	44	from	effects	850:856	arg1	composition					921:931	biochemical composition	909:931	biochemical composition	909:931	This study was aimed at determining the effects of glucose and fructose on cricket development and biochemical composition.
34707140	2	45	theme	low	243:245	arg1	concentrations					247:260	low concentrations	243:260	low concentrations	243:260	Its exogenicity and low concentrations mean that it can be regarded as one of the key regulatory molecules at the cellular as well as physiological level.
34707140	12	46	from	time	1479:1482	arg1	apparent					1547:1554	apparent	1547:1554	apparent	1547:1554	At the same time, a stronger detrimental effect of fructose than of glucose was apparent.
34707140	12	46	from	time	1479:1482	arg1	effect					1508:1513	a stronger detrimental effect	1485:1513	a stronger detrimental effect of fructose than of glucose	1485:1541	At the same time, a stronger detrimental effect of fructose than of glucose was apparent.
34707140	11	47	theme	white	1413:1417	arg1	strain					1419:1424	the white strain	1409:1424	the white strain	1409:1424	The results of the study indicate a stronger response of the white strain to HMD compared to the wild-type strain.
34707140	3	48	theme	molecules	492:500	arg1	production					452:461	the production	448:461	the production of other biologically active molecules	448:500	It has been shown to have a number of essential functions, such as in the production of other biologically active molecules.
34707140	10	49	from	changes	1290:1296	arg1	weight					1312:1317	imago body weight	1301:1317	imago body weight	1301:1317	However, no changes in imago body weight and water content were observed.
34707140	10	49	from	changes	1290:1296	arg1	content					1329:1335	water content	1323:1335	water content	1323:1335	However, no changes in imago body weight and water content were observed.
34707140	4	50	theme	monosaccharide	579:592	arg1	HMD					600:602	HMD	600:602	HMD	600:602	The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye.
34707140	4	50	theme	monosaccharide	579:592	arg1	diet					594:597	a high monosaccharide diet	572:597	a high monosaccharide diet (HMD)	572:603	The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye.
34707140	4	51	from	effects	561:567	arg1	domesticus					653:662	Acheta domesticus	646:662	Acheta domesticus	646:662	The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye.
34707140	4	51	from	effects	561:567	arg1	cricket					637:643	a hemimetabolic insect-house cricket	608:643	a hemimetabolic insect-house cricket (Acheta domesticus)	608:663	The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye.
34707140	4	51	from	effects	561:567	arg1	strain					678:683	a mutant strain	669:683	a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)	669:797	The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye.
34707140	0	52	theme	domesticus	137:146	arg1	strain					105:110	white-eyed mutant strain	87:110	white-eyed mutant strain of house cricket (Acheta domesticus)	87:147	The effects of high-monosaccharide diets on development and biochemical composition of white-eyed mutant strain of house cricket (Acheta domesticus).
34707140	8	53	theme	Biochemical	1123:1133	arg1	analyses					1135:1142	Biochemical analyses	1123:1142	Biochemical analyses	1123:1142	Biochemical analyses demonstrated the effects of HMD mainly on fat and glycogen content.
34707140	1	54	theme	essential	181:189	arg1	acids					197:201	the essential amino acids	177:201	the essential amino acids in the animal body	177:220	Tryptophan (TRP) is one of the essential amino acids in the animal body.
34707140	7	55	theme	strain	1077:1082	arg1	insects					1084:1090	white strain insects	1071:1090	white strain insects	1071:1090	ELISA assays indicated dysfunction of the TRP-KYN pathway in white strain insects and an elevated KYN/TRP ratio.
34707140	13	56	from	factor	1590:1595	arg1	response					1604:1611	the response	1600:1611	the response to HMD	1600:1618	Sex was found to be a modulating factor in the response to HMD.
34707140	4	57	theme	metabolic	780:788	arg1	KYN					775:777	KYN	775:777	KYN	775:777	The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye.
34707140	4	57	theme	metabolic	780:788	arg1	pathway					790:796	the tryptophan and kynurenine (KYN) metabolic pathway	744:796	pathway	790:796	The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye.
34707140	8	58	theme	HMD	1172:1174	arg1	effects					1161:1167	the effects	1157:1167	the effects of HMD mainly on fat and glycogen content	1157:1209	Biochemical analyses demonstrated the effects of HMD mainly on fat and glycogen content.
34707140	1	59	from	acids	197:201	arg1	body					217:220	the animal body	206:220	the animal body	206:220	Tryptophan (TRP) is one of the essential amino acids in the animal body.
34707140	4	60	theme	insect-house	624:635	arg1	domesticus					653:662	Acheta domesticus	646:662	Acheta domesticus	646:662	The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye.
34707140	4	60	theme	insect-house	624:635	arg1	cricket					637:643	a hemimetabolic insect-house cricket	608:643	a hemimetabolic insect-house cricket (Acheta domesticus)	608:663	The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye.
34707140	7	61	theme	elevated	1099:1106	arg1	ratio					1116:1120	an elevated KYN/TRP ratio	1096:1120	an elevated KYN/TRP ratio	1096:1120	ELISA assays indicated dysfunction of the TRP-KYN pathway in white strain insects and an elevated KYN/TRP ratio.
34707140	8	62	from	effects	1161:1167	arg1	fat					1186:1188	fat	1186:1188	fat	1186:1188	Biochemical analyses demonstrated the effects of HMD mainly on fat and glycogen content.
34707140	8	62	from	effects	1161:1167	arg1	content					1203:1209	glycogen content	1194:1209	glycogen content	1194:1209	Biochemical analyses demonstrated the effects of HMD mainly on fat and glycogen content.
34707140	12	63	theme	glucose	1535:1541	arg1	apparent					1547:1554	apparent	1547:1554	apparent	1547:1554	At the same time, a stronger detrimental effect of fructose than of glucose was apparent.
34707140	12	63	theme	glucose	1535:1541	arg1	effect					1508:1513	a stronger detrimental effect	1485:1513	a stronger detrimental effect of fructose than of glucose	1485:1541	At the same time, a stronger detrimental effect of fructose than of glucose was apparent.
34707140	9	64	located	observed	1247:1254	arg1	groups					1263:1268	the groups	1259:1268	the groups on HMD	1259:1275	A decrease in food intake was also observed in the groups on HMD.
34707140	9	64	located	observed	1247:1254	arg2	decrease					1214:1221	A decrease	1212:1221	A decrease in food intake	1212:1236	A decrease in food intake was also observed in the groups on HMD.
34707140	0	65	theme	white-eyed	87:96	arg1	strain					105:110	white-eyed mutant strain	87:110	white-eyed mutant strain of house cricket (Acheta domesticus)	87:147	The effects of high-monosaccharide diets on development and biochemical composition of white-eyed mutant strain of house cricket (Acheta domesticus).
34707140	12	66	theme	detrimental	1496:1506	arg1	apparent					1547:1554	apparent	1547:1554	apparent	1547:1554	At the same time, a stronger detrimental effect of fructose than of glucose was apparent.
34707140	12	66	theme	detrimental	1496:1506	arg1	effect					1508:1513	a stronger detrimental effect	1485:1513	a stronger detrimental effect of fructose than of glucose	1485:1541	At the same time, a stronger detrimental effect of fructose than of glucose was apparent.
34707140	0	67	from	effects	4:10	arg1	development					44:54	development	44:54	development	44:54	The effects of high-monosaccharide diets on development and biochemical composition of white-eyed mutant strain of house cricket (Acheta domesticus).
34707140	0	67	from	effects	4:10	arg1	composition					72:82	biochemical composition	60:82	biochemical composition	60:82	The effects of high-monosaccharide diets on development and biochemical composition of white-eyed mutant strain of house cricket (Acheta domesticus).
34707140	0	68	theme	strain	105:110	arg1	development					44:54	development	44:54	development	44:54	The effects of high-monosaccharide diets on development and biochemical composition of white-eyed mutant strain of house cricket (Acheta domesticus).
34707140	0	68	theme	strain	105:110	arg1	composition					72:82	biochemical composition	60:82	biochemical composition	60:82	The effects of high-monosaccharide diets on development and biochemical composition of white-eyed mutant strain of house cricket (Acheta domesticus).
34707140	0	69	theme	house	115:119	arg1	domesticus					137:146	house cricket (Acheta domesticus)	115:147	house cricket (Acheta domesticus)	115:147	The effects of high-monosaccharide diets on development and biochemical composition of white-eyed mutant strain of house cricket (Acheta domesticus).
34707140	4	70	theme	mutant	671:676	arg1	strain					678:683	a mutant strain	669:683	a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)	669:797	The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye.
34707140	2	71	theme	cellular	337:344	arg1	level					371:375	the cellular as well as physiological level	333:375	the cellular as well as physiological level	333:375	Its exogenicity and low concentrations mean that it can be regarded as one of the key regulatory molecules at the cellular as well as physiological level.
34707140	7	72	theme	TRP-KYN	1052:1058	arg1	pathway					1060:1066	the TRP-KYN pathway	1048:1066	the TRP-KYN pathway	1048:1066	ELISA assays indicated dysfunction of the TRP-KYN pathway in white strain insects and an elevated KYN/TRP ratio.
34707140	4	73	dep	cricket	637:643	arg1	eye					805:807	-white eye	798:807	-white eye	798:807	The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye.
34707140	10	74	theme	body	1307:1310	arg1	weight					1312:1317	imago body weight	1301:1317	imago body weight	1301:1317	However, no changes in imago body weight and water content were observed.
34707140	0	75	theme	high-monosaccharide	15:33	arg1	diets					35:39	high-monosaccharide diets	15:39	high-monosaccharide diets	15:39	The effects of high-monosaccharide diets on development and biochemical composition of white-eyed mutant strain of house cricket (Acheta domesticus).
34707140	2	76	theme	physiological	357:369	arg1	level					371:375	the cellular as well as physiological level	333:375	the cellular as well as physiological level	333:375	Its exogenicity and low concentrations mean that it can be regarded as one of the key regulatory molecules at the cellular as well as physiological level.
34707140	9	77	theme	food	1226:1229	arg1	intake					1231:1236	food intake	1226:1236	food intake	1226:1236	A decrease in food intake was also observed in the groups on HMD.
34707140	7	78	theme	ELISA	1010:1014	arg1	assays					1016:1021	ELISA assays	1010:1021	ELISA assays	1010:1021	ELISA assays indicated dysfunction of the TRP-KYN pathway in white strain insects and an elevated KYN/TRP ratio.
34707140	9	79	from	groups	1263:1268	arg1	HMD					1273:1275	HMD	1273:1275	HMD	1273:1275	A decrease in food intake was also observed in the groups on HMD.
34707140	5	80	theme	biochemical	909:919	arg1	composition					921:931	biochemical composition	909:931	biochemical composition	909:931	This study was aimed at determining the effects of glucose and fructose on cricket development and biochemical composition.
34707140	11	81	theme	strain	1419:1424	arg1	response					1397:1404	a stronger response	1386:1404	a stronger response of the white strain to HMD compared to the wild-type strain	1386:1464	The results of the study indicate a stronger response of the white strain to HMD compared to the wild-type strain.
34707140	3	82	theme	functions	426:434	arg1	number					406:411	a number	404:411	a number of essential functions	404:434	It has been shown to have a number of essential functions, such as in the production of other biologically active molecules.
34707140	4	83	theme	project	530:536	arg1	objective					512:520	The main objective	503:520	The main objective of this project	503:536	The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye.
34707140	11	84	theme	wild-type	1449:1457	arg1	strain					1459:1464	the wild-type strain	1445:1464	the wild-type strain	1445:1464	The results of the study indicate a stronger response of the white strain to HMD compared to the wild-type strain.
34707140	6	85	theme	cricket	986:992	arg1	strains					994:1000	both cricket strains	981:1000	both cricket strains	981:1000	A parallel goal was to compare the response of both cricket strains to HMD.
34707140	4	86	with	cricket	637:643	arg1	synthesis					714:722	impaired visual pigment synthesis	690:722	impaired visual pigment synthesis	690:722	The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye.
34707140	4	87	theme	visual	699:704	arg1	synthesis					714:722	impaired visual pigment synthesis	690:722	impaired visual pigment synthesis	690:722	The main objective of this project was to investigate the effects of a high monosaccharide diet (HMD) on a hemimetabolic insect-house cricket (Acheta domesticus) and a mutant strain with impaired visual pigment synthesis (closely related to the tryptophan and kynurenine (KYN) metabolic pathway)-white eye.
34707140	2	88	theme	regulatory	309:318	arg1	molecules					320:328	the key regulatory molecules	301:328	the key regulatory molecules at the cellular as well as physiological level	301:375	Its exogenicity and low concentrations mean that it can be regarded as one of the key regulatory molecules at the cellular as well as physiological level.
34707140	1	89	theme	animal	210:215	arg1	body					217:220	the animal body	206:220	the animal body	206:220	Tryptophan (TRP) is one of the essential amino acids in the animal body.
32504711	0	0	theme	bovine	85:90	arg1	bones					103:107	New Zealand bovine cancellous bones	73:107	New Zealand bovine cancellous bones for bone regeneration	73:129	Effect of chitosan infiltration on hydroxyapatite scaffolds derived from New Zealand bovine cancellous bones for bone regeneration.
32504711	1	1	theme	human	331:335	arg1	bone					337:340	human bone	331:340	human bone	331:340	Hydroxyapatite (HA) derived from bovine bones garnered wider interest as a bone substitute due to their abundant availability as meat wastes and similarities in morphology and mineral composition to human bone.
32504711	1	2	attach	derived	152:158	arg1	bones					172:176	bovine bones	165:176	bovine bones garnered wider interest as a bone	165:210	Hydroxyapatite (HA) derived from bovine bones garnered wider interest as a bone substitute due to their abundant availability as meat wastes and similarities in morphology and mineral composition to human bone.
32504711	1	2	attach	derived	152:158	arg2	HA					148:149	HA	148:149	HA	148:149	Hydroxyapatite (HA) derived from bovine bones garnered wider interest as a bone substitute due to their abundant availability as meat wastes and similarities in morphology and mineral composition to human bone.
32504711	1	2	attach	derived	152:158	arg2	Hydroxyapatite					132:145	Hydroxyapatite	132:145	Hydroxyapatite (HA) derived from bovine bones garnered wider interest as a bone	132:210	Hydroxyapatite (HA) derived from bovine bones garnered wider interest as a bone substitute due to their abundant availability as meat wastes and similarities in morphology and mineral composition to human bone.
32504711	5	3	theme	BHA	847:849	arg1	scaffolds					851:859	the BHA scaffolds	843:859	the BHA scaffolds	843:859	The presence of characteristic functional groups of HA and CS as detected by infrared spectroscopy confirmed the infiltration of CS into the BHA scaffolds.
32504711	1	4	theme	abundant	236:243	arg1	availability					245:256	their abundant availability	230:256	their abundant availability as meat wastes and similarities in morphology and mineral composition to human bone	230:340	Hydroxyapatite (HA) derived from bovine bones garnered wider interest as a bone substitute due to their abundant availability as meat wastes and similarities in morphology and mineral composition to human bone.
32504711	2	5	theme	cancellous	459:468	arg1	BHA					477:479	BHA	477:479	BHA	477:479	In our previous work, we developed an easy and reproducible method to prepare xenograft HA scaffolds from NZ bovine cancellous bones (BHA).
32504711	2	5	theme	cancellous	459:468	arg1	bones					470:474	NZ bovine cancellous bones	449:474	NZ bovine cancellous bones (BHA)	449:480	In our previous work, we developed an easy and reproducible method to prepare xenograft HA scaffolds from NZ bovine cancellous bones (BHA).
32504711	5	6	theme	functional	737:746	arg1	CS					765:766	CS	765:766	CS	765:766	The presence of characteristic functional groups of HA and CS as detected by infrared spectroscopy confirmed the infiltration of CS into the BHA scaffolds.
32504711	5	6	theme	functional	737:746	arg1	HA					758:759	HA	758:759	HA	758:759	The presence of characteristic functional groups of HA and CS as detected by infrared spectroscopy confirmed the infiltration of CS into the BHA scaffolds.
32504711	5	6	theme	functional	737:746	arg1	groups					748:753	characteristic functional groups	722:753	characteristic functional groups of HA and CS as detected by infrared spectroscopy	722:803	The presence of characteristic functional groups of HA and CS as detected by infrared spectroscopy confirmed the infiltration of CS into the BHA scaffolds.
32504711	5	7	theme	infrared	783:790	arg1	spectroscopy					792:803	infrared spectroscopy	783:803	infrared spectroscopy	783:803	The presence of characteristic functional groups of HA and CS as detected by infrared spectroscopy confirmed the infiltration of CS into the BHA scaffolds.
32504711	8	8	theme	CSHA	1130:1133	arg1	scaffolds					1135:1143	CSHA scaffolds	1130:1143	CSHA scaffolds	1130:1143	CSHA scaffolds presented good thermal, chemical and structural stability while demonstrating sustained biodegradability in simulated body fluid.
32504711	0	9	from	Effect	0:5	arg1	scaffolds					50:58	hydroxyapatite scaffolds	35:58	hydroxyapatite scaffolds derived from New Zealand bovine cancellous bones for bone regeneration	35:129	Effect of chitosan infiltration on hydroxyapatite scaffolds derived from New Zealand bovine cancellous bones for bone regeneration.
32504711	7	10	theme	porous	1066:1071	arg1	architecture					1073:1084	an interconnected porous architecture	1048:1084	an interconnected porous architecture	1048:1084	SEM and μCT analyses showed the CSHA scaffolds presented adequate porosity and an interconnected porous architecture required for cell migration and attachment.
32504711	10	11	theme	osteoblast	1413:1422	arg1	cells					1424:1428	Saos-2 osteoblast cells	1406:1428	Saos-2 osteoblast cells	1406:1428	CSHA scaffolds were biocompatible with Saos-2 osteoblast cells and supported cell proliferation significantly better than the BHA scaffolds indicating their potential in bone tissue engineering.
32504711	10	12	theme	CSHA	1367:1370	arg1	scaffolds					1372:1380	CSHA scaffolds	1367:1380	CSHA scaffolds	1367:1380	CSHA scaffolds were biocompatible with Saos-2 osteoblast cells and supported cell proliferation significantly better than the BHA scaffolds indicating their potential in bone tissue engineering.
32504711	6	13	theme	BHA	945:947	arg1	scaffolds					958:966	both BHA and CSHA scaffolds	940:966	both BHA and CSHA scaffolds	940:966	X-ray Diffraction study confirmed the presence of the hydroxyapatite phase in both BHA and CSHA scaffolds.
32504711	0	14	theme	cancellous	92:101	arg1	bones					103:107	New Zealand bovine cancellous bones	73:107	New Zealand bovine cancellous bones for bone regeneration	73:129	Effect of chitosan infiltration on hydroxyapatite scaffolds derived from New Zealand bovine cancellous bones for bone regeneration.
32504711	10	15	theme	Saos-2	1406:1411	arg1	cells					1424:1428	Saos-2 osteoblast cells	1406:1428	Saos-2 osteoblast cells	1406:1428	CSHA scaffolds were biocompatible with Saos-2 osteoblast cells and supported cell proliferation significantly better than the BHA scaffolds indicating their potential in bone tissue engineering.
32504711	10	16	theme	bone	1537:1540	arg1	engineering					1549:1559	bone tissue engineering	1537:1559	bone tissue engineering	1537:1559	CSHA scaffolds were biocompatible with Saos-2 osteoblast cells and supported cell proliferation significantly better than the BHA scaffolds indicating their potential in bone tissue engineering.
32504711	1	17	theme	meat	261:264	arg1	wastes					266:271	meat wastes	261:271	meat wastes	261:271	Hydroxyapatite (HA) derived from bovine bones garnered wider interest as a bone substitute due to their abundant availability as meat wastes and similarities in morphology and mineral composition to human bone.
32504711	0	18	theme	bone	113:116	arg1	regeneration					118:129	bone regeneration	113:129	bone regeneration	113:129	Effect of chitosan infiltration on hydroxyapatite scaffolds derived from New Zealand bovine cancellous bones for bone regeneration.
32504711	1	19	from	similarities	277:288	arg1	morphology					293:302	morphology	293:302	morphology	293:302	Hydroxyapatite (HA) derived from bovine bones garnered wider interest as a bone substitute due to their abundant availability as meat wastes and similarities in morphology and mineral composition to human bone.
32504711	1	19	from	similarities	277:288	arg1	composition					316:326	mineral composition	308:326	mineral composition	308:326	Hydroxyapatite (HA) derived from bovine bones garnered wider interest as a bone substitute due to their abundant availability as meat wastes and similarities in morphology and mineral composition to human bone.
32504711	4	20	theme	chitosan	611:618	arg1	infiltration					595:606	the infiltration	591:606	the infiltration of chitosan (CS) into the bovine HA scaffolds (CSHA)	591:659	The present study investigated the infiltration of chitosan (CS) into the bovine HA scaffolds (CSHA) to improve the mechanical properties of BHA.
32504711	9	21	theme	higher	1336:1341	arg1	properties					1311:1320	mechanical properties	1300:1320	mechanical properties significantly higher	1300:1341	CSHA scaffolds presented mechanical properties significantly higher than the BHA scaffolds.
32504711	1	22	theme	bovine	165:170	arg1	bones					172:176	bovine bones	165:176	bovine bones garnered wider interest as a bone	165:210	Hydroxyapatite (HA) derived from bovine bones garnered wider interest as a bone substitute due to their abundant availability as meat wastes and similarities in morphology and mineral composition to human bone.
32504711	4	23	theme	present	564:570	arg1	study					572:576	The present study	560:576	The present study	560:576	The present study investigated the infiltration of chitosan (CS) into the bovine HA scaffolds (CSHA) to improve the mechanical properties of BHA.
32504711	9	24	theme	mechanical	1300:1309	arg1	properties					1311:1320	mechanical properties	1300:1320	mechanical properties significantly higher	1300:1341	CSHA scaffolds presented mechanical properties significantly higher than the BHA scaffolds.
32504711	2	25	theme	bovine	452:457	arg1	BHA					477:479	BHA	477:479	BHA	477:479	In our previous work, we developed an easy and reproducible method to prepare xenograft HA scaffolds from NZ bovine cancellous bones (BHA).
32504711	2	25	theme	bovine	452:457	arg1	bones					470:474	NZ bovine cancellous bones	449:474	NZ bovine cancellous bones (BHA)	449:480	In our previous work, we developed an easy and reproducible method to prepare xenograft HA scaffolds from NZ bovine cancellous bones (BHA).
32504711	3	26	theme	processing	496:505	arg1	methodology					507:517	the processing methodology	492:517	the processing methodology	492:517	However, the processing methodology rendered the material mechanically weak.
32504711	7	27	dep	showed	990:995	arg1	presented					1016:1024	presented	1016:1024	showed the CSHA scaffolds presented adequate porosity and an interconnected porous architecture required for cell migration and attachment	990:1127	SEM and μCT analyses showed the CSHA scaffolds presented adequate porosity and an interconnected porous architecture required for cell migration and attachment.
32504711	2	28	theme	HA	431:432	arg1	scaffolds					434:442	xenograft HA scaffolds	421:442	xenograft HA scaffolds from NZ bovine cancellous bones (BHA)	421:480	In our previous work, we developed an easy and reproducible method to prepare xenograft HA scaffolds from NZ bovine cancellous bones (BHA).
32504711	0	29	theme	Zealand	77:83	arg1	bones					103:107	New Zealand bovine cancellous bones	73:107	New Zealand bovine cancellous bones for bone regeneration	73:129	Effect of chitosan infiltration on hydroxyapatite scaffolds derived from New Zealand bovine cancellous bones for bone regeneration.
32504711	1	30	from	wastes	266:271	arg1	morphology					293:302	morphology	293:302	morphology	293:302	Hydroxyapatite (HA) derived from bovine bones garnered wider interest as a bone substitute due to their abundant availability as meat wastes and similarities in morphology and mineral composition to human bone.
32504711	1	30	from	wastes	266:271	arg1	composition					316:326	mineral composition	308:326	mineral composition	308:326	Hydroxyapatite (HA) derived from bovine bones garnered wider interest as a bone substitute due to their abundant availability as meat wastes and similarities in morphology and mineral composition to human bone.
32504711	10	31	theme	cell	1444:1447	arg1	proliferation					1449:1461	cell proliferation	1444:1461	cell proliferation significantly better than the BHA scaffolds indicating their potential in bone tissue engineering	1444:1559	CSHA scaffolds were biocompatible with Saos-2 osteoblast cells and supported cell proliferation significantly better than the BHA scaffolds indicating their potential in bone tissue engineering.
32504711	4	32	theme	HA	641:642	arg1	CSHA					655:658	CSHA	655:658	CSHA	655:658	The present study investigated the infiltration of chitosan (CS) into the bovine HA scaffolds (CSHA) to improve the mechanical properties of BHA.
32504711	4	32	theme	HA	641:642	arg1	scaffolds					644:652	the bovine HA scaffolds	630:652	the bovine HA scaffolds (CSHA)	630:659	The present study investigated the infiltration of chitosan (CS) into the bovine HA scaffolds (CSHA) to improve the mechanical properties of BHA.
32504711	2	33	theme	xenograft	421:429	arg1	scaffolds					434:442	xenograft HA scaffolds	421:442	xenograft HA scaffolds from NZ bovine cancellous bones (BHA)	421:480	In our previous work, we developed an easy and reproducible method to prepare xenograft HA scaffolds from NZ bovine cancellous bones (BHA).
32504711	8	34	theme	sustained	1223:1231	arg1	biodegradability					1233:1248	sustained biodegradability	1223:1248	sustained biodegradability in simulated body fluid	1223:1272	CSHA scaffolds presented good thermal, chemical and structural stability while demonstrating sustained biodegradability in simulated body fluid.
32504711	3	35	theme	weak	554:557	arg1	material					532:539	the material	528:539	the material mechanically weak	528:557	However, the processing methodology rendered the material mechanically weak.
32504711	6	36	theme	CSHA	953:956	arg1	scaffolds					958:966	both BHA and CSHA scaffolds	940:966	both BHA and CSHA scaffolds	940:966	X-ray Diffraction study confirmed the presence of the hydroxyapatite phase in both BHA and CSHA scaffolds.
32504711	1	37	theme	wider	187:191	arg1	interest					193:200	wider interest	187:200	wider interest	187:200	Hydroxyapatite (HA) derived from bovine bones garnered wider interest as a bone substitute due to their abundant availability as meat wastes and similarities in morphology and mineral composition to human bone.
32504711	1	37	theme	wider	187:191	arg1	bone					207:210	a bone	205:210	a bone	205:210	Hydroxyapatite (HA) derived from bovine bones garnered wider interest as a bone substitute due to their abundant availability as meat wastes and similarities in morphology and mineral composition to human bone.
32504711	0	38	theme	infiltration	19:30	arg1	Effect					0:5	Effect	0:5	Effect of chitosan infiltration on hydroxyapatite scaffolds derived from New Zealand bovine cancellous bones for bone regeneration.	0:130	Effect of chitosan infiltration on hydroxyapatite scaffolds derived from New Zealand bovine cancellous bones for bone regeneration.
32504711	5	39	theme	CS	765:766	arg1	CS					765:766	CS	765:766	CS	765:766	The presence of characteristic functional groups of HA and CS as detected by infrared spectroscopy confirmed the infiltration of CS into the BHA scaffolds.
32504711	5	39	theme	CS	765:766	arg1	HA					758:759	HA	758:759	HA	758:759	The presence of characteristic functional groups of HA and CS as detected by infrared spectroscopy confirmed the infiltration of CS into the BHA scaffolds.
32504711	5	39	theme	CS	765:766	arg1	groups					748:753	characteristic functional groups	722:753	characteristic functional groups of HA and CS as detected by infrared spectroscopy	722:803	The presence of characteristic functional groups of HA and CS as detected by infrared spectroscopy confirmed the infiltration of CS into the BHA scaffolds.
32504711	10	40	theme	tissue	1542:1547	arg1	engineering					1549:1559	bone tissue engineering	1537:1559	bone tissue engineering	1537:1559	CSHA scaffolds were biocompatible with Saos-2 osteoblast cells and supported cell proliferation significantly better than the BHA scaffolds indicating their potential in bone tissue engineering.
32504711	0	41	attach	derived	60:66	arg1	bones					103:107	New Zealand bovine cancellous bones	73:107	New Zealand bovine cancellous bones for bone regeneration	73:129	Effect of chitosan infiltration on hydroxyapatite scaffolds derived from New Zealand bovine cancellous bones for bone regeneration.
32504711	0	41	attach	derived	60:66	arg2	scaffolds					50:58	hydroxyapatite scaffolds	35:58	hydroxyapatite scaffolds derived from New Zealand bovine cancellous bones for bone regeneration	35:129	Effect of chitosan infiltration on hydroxyapatite scaffolds derived from New Zealand bovine cancellous bones for bone regeneration.
32504711	8	42	theme	structural	1182:1191	arg1	stability					1193:1201	good thermal, chemical and structural stability	1155:1201	good thermal, chemical and structural stability	1155:1201	CSHA scaffolds presented good thermal, chemical and structural stability while demonstrating sustained biodegradability in simulated body fluid.
32504711	7	43	theme	interconnected	1051:1064	arg1	architecture					1073:1084	an interconnected porous architecture	1048:1084	an interconnected porous architecture	1048:1084	SEM and μCT analyses showed the CSHA scaffolds presented adequate porosity and an interconnected porous architecture required for cell migration and attachment.
32504711	8	44	theme	body	1263:1266	arg1	fluid					1268:1272	simulated body fluid	1253:1272	simulated body fluid	1253:1272	CSHA scaffolds presented good thermal, chemical and structural stability while demonstrating sustained biodegradability in simulated body fluid.
32504711	0	45	theme	chitosan	10:17	arg1	infiltration					19:30	chitosan infiltration	10:30	chitosan infiltration	10:30	Effect of chitosan infiltration on hydroxyapatite scaffolds derived from New Zealand bovine cancellous bones for bone regeneration.
32504711	6	46	theme	hydroxyapatite	916:929	arg1	phase					931:935	the hydroxyapatite phase	912:935	the hydroxyapatite phase	912:935	X-ray Diffraction study confirmed the presence of the hydroxyapatite phase in both BHA and CSHA scaffolds.
32504711	7	47	theme	adequate	1026:1033	arg1	porosity					1035:1042	adequate porosity	1026:1042	adequate porosity	1026:1042	SEM and μCT analyses showed the CSHA scaffolds presented adequate porosity and an interconnected porous architecture required for cell migration and attachment.
32504711	2	48	from	bones	470:474	arg1	scaffolds					434:442	xenograft HA scaffolds	421:442	xenograft HA scaffolds from NZ bovine cancellous bones (BHA)	421:480	In our previous work, we developed an easy and reproducible method to prepare xenograft HA scaffolds from NZ bovine cancellous bones (BHA).
32504711	4	49	theme	BHA	701:703	arg1	properties					687:696	the mechanical properties	672:696	the mechanical properties of BHA	672:703	The present study investigated the infiltration of chitosan (CS) into the bovine HA scaffolds (CSHA) to improve the mechanical properties of BHA.
32504711	6	50	theme	Diffraction	868:878	arg1	study					880:884	X-ray Diffraction study	862:884	X-ray Diffraction study	862:884	X-ray Diffraction study confirmed the presence of the hydroxyapatite phase in both BHA and CSHA scaffolds.
32504711	2	51	theme	reproducible	390:401	arg1	method					403:408	an easy and reproducible method	378:408	an easy and reproducible method to prepare xenograft HA scaffolds from NZ bovine cancellous bones (BHA)	378:480	In our previous work, we developed an easy and reproducible method to prepare xenograft HA scaffolds from NZ bovine cancellous bones (BHA).
32504711	0	52	theme	hydroxyapatite	35:48	arg1	scaffolds					50:58	hydroxyapatite scaffolds	35:58	hydroxyapatite scaffolds derived from New Zealand bovine cancellous bones for bone regeneration	35:129	Effect of chitosan infiltration on hydroxyapatite scaffolds derived from New Zealand bovine cancellous bones for bone regeneration.
32504711	7	53	theme	CSHA	1001:1004	arg1	scaffolds					1006:1014	the CSHA scaffolds	997:1014	the CSHA scaffolds	997:1014	SEM and μCT analyses showed the CSHA scaffolds presented adequate porosity and an interconnected porous architecture required for cell migration and attachment.
32504711	6	54	attach	presence	900:907	arg2	phase					931:935	the hydroxyapatite phase	912:935	the hydroxyapatite phase	912:935	X-ray Diffraction study confirmed the presence of the hydroxyapatite phase in both BHA and CSHA scaffolds.
32504711	6	54	attach	presence	900:907	arg1	scaffolds					958:966	both BHA and CSHA scaffolds	940:966	both BHA and CSHA scaffolds	940:966	X-ray Diffraction study confirmed the presence of the hydroxyapatite phase in both BHA and CSHA scaffolds.
32504711	4	55	theme	bovine	634:639	arg1	CSHA					655:658	CSHA	655:658	CSHA	655:658	The present study investigated the infiltration of chitosan (CS) into the bovine HA scaffolds (CSHA) to improve the mechanical properties of BHA.
32504711	4	55	theme	bovine	634:639	arg1	scaffolds					644:652	the bovine HA scaffolds	630:652	the bovine HA scaffolds (CSHA)	630:659	The present study investigated the infiltration of chitosan (CS) into the bovine HA scaffolds (CSHA) to improve the mechanical properties of BHA.
32504711	9	56	theme	BHA	1352:1354	arg1	scaffolds					1356:1364	the BHA scaffolds	1348:1364	the BHA scaffolds	1348:1364	CSHA scaffolds presented mechanical properties significantly higher than the BHA scaffolds.
32504711	2	57	theme	NZ	449:450	arg1	BHA					477:479	BHA	477:479	BHA	477:479	In our previous work, we developed an easy and reproducible method to prepare xenograft HA scaffolds from NZ bovine cancellous bones (BHA).
32504711	2	57	theme	NZ	449:450	arg1	bones					470:474	NZ bovine cancellous bones	449:474	NZ bovine cancellous bones (BHA)	449:480	In our previous work, we developed an easy and reproducible method to prepare xenograft HA scaffolds from NZ bovine cancellous bones (BHA).
32504711	5	58	theme	groups	748:753	arg1	presence					710:717	The presence	706:717	The presence of characteristic functional groups of HA and CS as detected by infrared spectroscopy	706:803	The presence of characteristic functional groups of HA and CS as detected by infrared spectroscopy confirmed the infiltration of CS into the BHA scaffolds.
32504711	8	59	theme	chemical	1169:1176	arg1	stability					1193:1201	good thermal, chemical and structural stability	1155:1201	good thermal, chemical and structural stability	1155:1201	CSHA scaffolds presented good thermal, chemical and structural stability while demonstrating sustained biodegradability in simulated body fluid.
32504711	7	60	theme	SEM	969:971	arg1	analyses					981:988	SEM and μCT analyses	969:988	SEM and μCT analyses	969:988	SEM and μCT analyses showed the CSHA scaffolds presented adequate porosity and an interconnected porous architecture required for cell migration and attachment.
32504711	2	61	theme	easy	381:384	arg1	method					403:408	an easy and reproducible method	378:408	an easy and reproducible method to prepare xenograft HA scaffolds from NZ bovine cancellous bones (BHA)	378:480	In our previous work, we developed an easy and reproducible method to prepare xenograft HA scaffolds from NZ bovine cancellous bones (BHA).
32504711	7	62	theme	cell	1099:1102	arg1	migration					1104:1112	cell migration	1099:1112	cell migration	1099:1112	SEM and μCT analyses showed the CSHA scaffolds presented adequate porosity and an interconnected porous architecture required for cell migration and attachment.
32504711	6	63	theme	phase	931:935	arg1	presence					900:907	the presence	896:907	the presence of the hydroxyapatite phase in both BHA and CSHA scaffolds	896:966	X-ray Diffraction study confirmed the presence of the hydroxyapatite phase in both BHA and CSHA scaffolds.
32504711	8	64	from	biodegradability	1233:1248	arg1	fluid					1268:1272	simulated body fluid	1253:1272	simulated body fluid	1253:1272	CSHA scaffolds presented good thermal, chemical and structural stability while demonstrating sustained biodegradability in simulated body fluid.
32504711	5	65	theme	HA	758:759	arg1	CS					765:766	CS	765:766	CS	765:766	The presence of characteristic functional groups of HA and CS as detected by infrared spectroscopy confirmed the infiltration of CS into the BHA scaffolds.
32504711	5	65	theme	HA	758:759	arg1	HA					758:759	HA	758:759	HA	758:759	The presence of characteristic functional groups of HA and CS as detected by infrared spectroscopy confirmed the infiltration of CS into the BHA scaffolds.
32504711	5	65	theme	HA	758:759	arg1	groups					748:753	characteristic functional groups	722:753	characteristic functional groups of HA and CS as detected by infrared spectroscopy	722:803	The presence of characteristic functional groups of HA and CS as detected by infrared spectroscopy confirmed the infiltration of CS into the BHA scaffolds.
32504711	1	66	theme	mineral	308:314	arg1	composition					316:326	mineral composition	308:326	mineral composition	308:326	Hydroxyapatite (HA) derived from bovine bones garnered wider interest as a bone substitute due to their abundant availability as meat wastes and similarities in morphology and mineral composition to human bone.
32504711	6	67	theme	X-ray	862:866	arg1	Diffraction					868:878	X-ray Diffraction	862:878	X-ray Diffraction study	862:884	X-ray Diffraction study confirmed the presence of the hydroxyapatite phase in both BHA and CSHA scaffolds.
32504711	5	68	dep	HA	758:759	arg1	detected					771:778	detected	771:778	detected by infrared spectroscopy	771:803	The presence of characteristic functional groups of HA and CS as detected by infrared spectroscopy confirmed the infiltration of CS into the BHA scaffolds.
32504711	10	69	theme	better	1477:1482	arg1	proliferation					1449:1461	cell proliferation	1444:1461	cell proliferation significantly better than the BHA scaffolds indicating their potential in bone tissue engineering	1444:1559	CSHA scaffolds were biocompatible with Saos-2 osteoblast cells and supported cell proliferation significantly better than the BHA scaffolds indicating their potential in bone tissue engineering.
32504711	8	70	theme	thermal	1160:1166	arg1	stability					1193:1201	good thermal, chemical and structural stability	1155:1201	good thermal, chemical and structural stability	1155:1201	CSHA scaffolds presented good thermal, chemical and structural stability while demonstrating sustained biodegradability in simulated body fluid.
32504711	7	71	theme	μCT	977:979	arg1	analyses					981:988	SEM and μCT analyses	969:988	SEM and μCT analyses	969:988	SEM and μCT analyses showed the CSHA scaffolds presented adequate porosity and an interconnected porous architecture required for cell migration and attachment.
32504711	4	72	theme	mechanical	676:685	arg1	properties					687:696	the mechanical properties	672:696	the mechanical properties of BHA	672:703	The present study investigated the infiltration of chitosan (CS) into the bovine HA scaffolds (CSHA) to improve the mechanical properties of BHA.
32504711	5	73	theme	CS	835:836	arg1	infiltration					819:830	the infiltration	815:830	the infiltration of CS into the BHA scaffolds	815:859	The presence of characteristic functional groups of HA and CS as detected by infrared spectroscopy confirmed the infiltration of CS into the BHA scaffolds.
32504711	6	74	from	presence	900:907	arg1	scaffolds					958:966	both BHA and CSHA scaffolds	940:966	both BHA and CSHA scaffolds	940:966	X-ray Diffraction study confirmed the presence of the hydroxyapatite phase in both BHA and CSHA scaffolds.
32504711	8	75	theme	good	1155:1158	arg1	stability					1193:1201	good thermal, chemical and structural stability	1155:1201	good thermal, chemical and structural stability	1155:1201	CSHA scaffolds presented good thermal, chemical and structural stability while demonstrating sustained biodegradability in simulated body fluid.
32504711	5	76	theme	characteristic	722:735	arg1	CS					765:766	CS	765:766	CS	765:766	The presence of characteristic functional groups of HA and CS as detected by infrared spectroscopy confirmed the infiltration of CS into the BHA scaffolds.
32504711	5	76	theme	characteristic	722:735	arg1	HA					758:759	HA	758:759	HA	758:759	The presence of characteristic functional groups of HA and CS as detected by infrared spectroscopy confirmed the infiltration of CS into the BHA scaffolds.
32504711	5	76	theme	characteristic	722:735	arg1	groups					748:753	characteristic functional groups	722:753	characteristic functional groups of HA and CS as detected by infrared spectroscopy	722:803	The presence of characteristic functional groups of HA and CS as detected by infrared spectroscopy confirmed the infiltration of CS into the BHA scaffolds.
32504711	10	77	from	potential	1524:1532	arg1	engineering					1549:1559	bone tissue engineering	1537:1559	bone tissue engineering	1537:1559	CSHA scaffolds were biocompatible with Saos-2 osteoblast cells and supported cell proliferation significantly better than the BHA scaffolds indicating their potential in bone tissue engineering.
32504711	0	78	theme	New	73:75	arg1	bones					103:107	New Zealand bovine cancellous bones	73:107	New Zealand bovine cancellous bones for bone regeneration	73:129	Effect of chitosan infiltration on hydroxyapatite scaffolds derived from New Zealand bovine cancellous bones for bone regeneration.
32504711	10	79	theme	BHA	1493:1495	arg1	scaffolds					1497:1505	the BHA scaffolds	1489:1505	the BHA scaffolds indicating their potential in bone tissue engineering	1489:1559	CSHA scaffolds were biocompatible with Saos-2 osteoblast cells and supported cell proliferation significantly better than the BHA scaffolds indicating their potential in bone tissue engineering.
32504711	8	80	theme	simulated	1253:1261	arg1	fluid					1268:1272	simulated body fluid	1253:1272	simulated body fluid	1253:1272	CSHA scaffolds presented good thermal, chemical and structural stability while demonstrating sustained biodegradability in simulated body fluid.
32504711	10	81	with	biocompatible	1387:1399	arg1	cells					1424:1428	Saos-2 osteoblast cells	1406:1428	Saos-2 osteoblast cells	1406:1428	CSHA scaffolds were biocompatible with Saos-2 osteoblast cells and supported cell proliferation significantly better than the BHA scaffolds indicating their potential in bone tissue engineering.
32504711	9	82	theme	CSHA	1275:1278	arg1	scaffolds					1280:1288	CSHA scaffolds	1275:1288	CSHA scaffolds	1275:1288	CSHA scaffolds presented mechanical properties significantly higher than the BHA scaffolds.
32504711	2	83	theme	previous	350:357	arg1	work					359:362	our previous work	346:362	our previous work	346:362	In our previous work, we developed an easy and reproducible method to prepare xenograft HA scaffolds from NZ bovine cancellous bones (BHA).
32238096	3	0	theme	Fourier-Transform	933:949	arg1	Spectroscopy					960:971	Fourier-Transform Infrared Spectroscopy	933:971	Fourier-Transform Infrared Spectroscopy (FTIR)	933:978	The proposed approach is composed of 4 steps: (1) Collecting data about consumed medical devices to be categorized into the residues Groups (A and E); (2) Identifying the polymeric composition with information provided by suppliers; (3) Characterizing the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR) and (4) Determining the polymer melting point by Differential Scanning Calorimetry (DSC).
32238096	3	0	theme	Fourier-Transform	933:949	arg1	FTIR					974:977	FTIR	974:977	FTIR	974:977	The proposed approach is composed of 4 steps: (1) Collecting data about consumed medical devices to be categorized into the residues Groups (A and E); (2) Identifying the polymeric composition with information provided by suppliers; (3) Characterizing the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR) and (4) Determining the polymer melting point by Differential Scanning Calorimetry (DSC).
32238096	1	1	theme	university	279:288	arg1	hospital					290:297	a Brazilian university hospital	267:297	a Brazilian university hospital	267:297	This work presents the health-care waste (HCW) management and an approach to assess and identify polymers in a General Surgery Unit - Internment Service (GSU) of a Brazilian university hospital, to estimate the main polymers presenting in medical devices that are consumed during a year, discarded either as infecting (Group A) or as scarifying residue (Group E).
32238096	2	2	theme	medical	503:509	arg1	devices					511:517	the medical devices	499:517	the medical devices	499:517	Among the waste produced from the medical devices, 3.14 ton (98.79%) were composed of polymers (63.06% of plastics and 35.73% elastomers) while around 0.03 ton (1.21%) by metals.
32238096	3	3	dep	categorized	751:761	arg1	A					789:789	A	789:789	A	789:789	The proposed approach is composed of 4 steps: (1) Collecting data about consumed medical devices to be categorized into the residues Groups (A and E); (2) Identifying the polymeric composition with information provided by suppliers; (3) Characterizing the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR) and (4) Determining the polymer melting point by Differential Scanning Calorimetry (DSC).
32238096	3	3	dep	categorized	751:761	arg1	E					795:795	E	795:795	E	795:795	The proposed approach is composed of 4 steps: (1) Collecting data about consumed medical devices to be categorized into the residues Groups (A and E); (2) Identifying the polymeric composition with information provided by suppliers; (3) Characterizing the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR) and (4) Determining the polymer melting point by Differential Scanning Calorimetry (DSC).
32238096	1	4	theme	waste	140:144	arg1	management					152:161	the health-care waste (HCW) management	124:161	the health-care waste (HCW) management	124:161	This work presents the health-care waste (HCW) management and an approach to assess and identify polymers in a General Surgery Unit - Internment Service (GSU) of a Brazilian university hospital, to estimate the main polymers presenting in medical devices that are consumed during a year, discarded either as infecting (Group A) or as scarifying residue (Group E).
32238096	3	5	theme	Collecting	698:707	arg1	data					709:712	(1) Collecting data	694:712	(1) Collecting data	694:712	The proposed approach is composed of 4 steps: (1) Collecting data about consumed medical devices to be categorized into the residues Groups (A and E); (2) Identifying the polymeric composition with information provided by suppliers; (3) Characterizing the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR) and (4) Determining the polymer melting point by Differential Scanning Calorimetry (DSC).
32238096	4	6	theme	high-density	1160:1171	arg1	polyethylene					1173:1184	high-density polyethylene	1160:1184	high-density polyethylene (5.28%)	1160:1192	According to the results, the analyzed HCW was composed mainly of polypropylene (80.88%), high-density polyethylene (5.28%), polystyrene (4.51%), and cellulose (3.58%), from a total of 11 different polymers.
32238096	4	6	theme	high-density	1160:1171	arg1	%					1191:1191	5.28%	1187:1191	5.28%	1187:1191	According to the results, the analyzed HCW was composed mainly of polypropylene (80.88%), high-density polyethylene (5.28%), polystyrene (4.51%), and cellulose (3.58%), from a total of 11 different polymers.
32238096	3	7	theme	Infrared	951:958	arg1	Spectroscopy					960:971	Fourier-Transform Infrared Spectroscopy	933:971	Fourier-Transform Infrared Spectroscopy (FTIR)	933:978	The proposed approach is composed of 4 steps: (1) Collecting data about consumed medical devices to be categorized into the residues Groups (A and E); (2) Identifying the polymeric composition with information provided by suppliers; (3) Characterizing the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR) and (4) Determining the polymer melting point by Differential Scanning Calorimetry (DSC).
32238096	3	7	theme	Infrared	951:958	arg1	FTIR					974:977	FTIR	974:977	FTIR	974:977	The proposed approach is composed of 4 steps: (1) Collecting data about consumed medical devices to be categorized into the residues Groups (A and E); (2) Identifying the polymeric composition with information provided by suppliers; (3) Characterizing the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR) and (4) Determining the polymer melting point by Differential Scanning Calorimetry (DSC).
32238096	1	8	theme	hospital	290:297	arg1	Unit					232:235	a General Surgery Unit	214:235	a General Surgery Unit	214:235	This work presents the health-care waste (HCW) management and an approach to assess and identify polymers in a General Surgery Unit - Internment Service (GSU) of a Brazilian university hospital, to estimate the main polymers presenting in medical devices that are consumed during a year, discarded either as infecting (Group A) or as scarifying residue (Group E).
32238096	1	9	theme	General	216:222	arg1	Unit					232:235	a General Surgery Unit	214:235	a General Surgery Unit	214:235	This work presents the health-care waste (HCW) management and an approach to assess and identify polymers in a General Surgery Unit - Internment Service (GSU) of a Brazilian university hospital, to estimate the main polymers presenting in medical devices that are consumed during a year, discarded either as infecting (Group A) or as scarifying residue (Group E).
32238096	0	10	theme	Brazilian	74:82	arg1	hospital					95:102	a Brazilian university hospital	72:102	a Brazilian university hospital	72:102	An approach to assess and identify polymers in the health-care waste of a Brazilian university hospital.
32238096	3	11	dep	Determining	988:998	arg1	4					985:985	4	985:985	4	985:985	The proposed approach is composed of 4 steps: (1) Collecting data about consumed medical devices to be categorized into the residues Groups (A and E); (2) Identifying the polymeric composition with information provided by suppliers; (3) Characterizing the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR) and (4) Determining the polymer melting point by Differential Scanning Calorimetry (DSC).
32238096	1	12	theme	Surgery	224:230	arg1	Unit					232:235	a General Surgery Unit	214:235	a General Surgery Unit	214:235	This work presents the health-care waste (HCW) management and an approach to assess and identify polymers in a General Surgery Unit - Internment Service (GSU) of a Brazilian university hospital, to estimate the main polymers presenting in medical devices that are consumed during a year, discarded either as infecting (Group A) or as scarifying residue (Group E).
32238096	0	13	theme	hospital	95:102	arg1	waste					63:67	the health-care waste	47:67	the health-care waste of a Brazilian university hospital	47:102	An approach to assess and identify polymers in the health-care waste of a Brazilian university hospital.
32238096	1	14	theme	Group	459:463	arg1	E					465:465	Group E	459:465	Group E	459:465	This work presents the health-care waste (HCW) management and an approach to assess and identify polymers in a General Surgery Unit - Internment Service (GSU) of a Brazilian university hospital, to estimate the main polymers presenting in medical devices that are consumed during a year, discarded either as infecting (Group A) or as scarifying residue (Group E).
32238096	1	14	theme	Group	459:463	arg1	residue					450:456	scarifying residue	439:456	scarifying residue (Group E)	439:466	This work presents the health-care waste (HCW) management and an approach to assess and identify polymers in a General Surgery Unit - Internment Service (GSU) of a Brazilian university hospital, to estimate the main polymers presenting in medical devices that are consumed during a year, discarded either as infecting (Group A) or as scarifying residue (Group E).
32238096	1	15	theme	HCW	147:149	arg1	management					152:161	the health-care waste (HCW) management	124:161	the health-care waste (HCW) management	124:161	This work presents the health-care waste (HCW) management and an approach to assess and identify polymers in a General Surgery Unit - Internment Service (GSU) of a Brazilian university hospital, to estimate the main polymers presenting in medical devices that are consumed during a year, discarded either as infecting (Group A) or as scarifying residue (Group E).
32238096	0	16	theme	university	84:93	arg1	hospital					95:102	a Brazilian university hospital	72:102	a Brazilian university hospital	72:102	An approach to assess and identify polymers in the health-care waste of a Brazilian university hospital.
32238096	1	17	theme	main	316:319	arg1	polymers					321:328	the main polymers	312:328	the main polymers presenting in medical devices that are consumed during a year, discarded either as infecting (Group A) or as scarifying residue (Group E)	312:466	This work presents the health-care waste (HCW) management and an approach to assess and identify polymers in a General Surgery Unit - Internment Service (GSU) of a Brazilian university hospital, to estimate the main polymers presenting in medical devices that are consumed during a year, discarded either as infecting (Group A) or as scarifying residue (Group E).
32238096	3	18	theme	polymeric	819:827	arg1	composition					829:839	the polymeric composition	815:839	the polymeric composition with information provided by suppliers	815:878	The proposed approach is composed of 4 steps: (1) Collecting data about consumed medical devices to be categorized into the residues Groups (A and E); (2) Identifying the polymeric composition with information provided by suppliers; (3) Characterizing the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR) and (4) Determining the polymer melting point by Differential Scanning Calorimetry (DSC).
32238096	3	19	theme	Differential	1029:1040	arg1	Calorimetry					1051:1061	Differential Scanning Calorimetry	1029:1061	Differential Scanning Calorimetry (DSC)	1029:1067	The proposed approach is composed of 4 steps: (1) Collecting data about consumed medical devices to be categorized into the residues Groups (A and E); (2) Identifying the polymeric composition with information provided by suppliers; (3) Characterizing the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR) and (4) Determining the polymer melting point by Differential Scanning Calorimetry (DSC).
32238096	3	19	theme	Differential	1029:1040	arg1	DSC					1064:1066	DSC	1064:1066	DSC	1064:1066	The proposed approach is composed of 4 steps: (1) Collecting data about consumed medical devices to be categorized into the residues Groups (A and E); (2) Identifying the polymeric composition with information provided by suppliers; (3) Characterizing the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR) and (4) Determining the polymer melting point by Differential Scanning Calorimetry (DSC).
32238096	3	20	dep	data	709:712	arg1	1					695:695	1	695:695	1	695:695	The proposed approach is composed of 4 steps: (1) Collecting data about consumed medical devices to be categorized into the residues Groups (A and E); (2) Identifying the polymeric composition with information provided by suppliers; (3) Characterizing the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR) and (4) Determining the polymer melting point by Differential Scanning Calorimetry (DSC).
32238096	2	21	dep	%	570:570	arg1	elastomers					595:604	elastomers	595:604	elastomers	595:604	Among the waste produced from the medical devices, 3.14 ton (98.79%) were composed of polymers (63.06% of plastics and 35.73% elastomers) while around 0.03 ton (1.21%) by metals.
32238096	3	22	theme	Scanning	1042:1049	arg1	Calorimetry					1051:1061	Differential Scanning Calorimetry	1029:1061	Differential Scanning Calorimetry (DSC)	1029:1067	The proposed approach is composed of 4 steps: (1) Collecting data about consumed medical devices to be categorized into the residues Groups (A and E); (2) Identifying the polymeric composition with information provided by suppliers; (3) Characterizing the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR) and (4) Determining the polymer melting point by Differential Scanning Calorimetry (DSC).
32238096	3	22	theme	Scanning	1042:1049	arg1	DSC					1064:1066	DSC	1064:1066	DSC	1064:1066	The proposed approach is composed of 4 steps: (1) Collecting data about consumed medical devices to be categorized into the residues Groups (A and E); (2) Identifying the polymeric composition with information provided by suppliers; (3) Characterizing the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR) and (4) Determining the polymer melting point by Differential Scanning Calorimetry (DSC).
32238096	1	23	theme	Internment	239:248	arg1	Service					250:256	Internment Service	239:256	Internment Service (GSU)	239:262	This work presents the health-care waste (HCW) management and an approach to assess and identify polymers in a General Surgery Unit - Internment Service (GSU) of a Brazilian university hospital, to estimate the main polymers presenting in medical devices that are consumed during a year, discarded either as infecting (Group A) or as scarifying residue (Group E).
32238096	1	23	theme	Internment	239:248	arg1	GSU					259:261	GSU	259:261	GSU	259:261	This work presents the health-care waste (HCW) management and an approach to assess and identify polymers in a General Surgery Unit - Internment Service (GSU) of a Brazilian university hospital, to estimate the main polymers presenting in medical devices that are consumed during a year, discarded either as infecting (Group A) or as scarifying residue (Group E).
32238096	4	24	theme	polymers	1268:1275	arg1	total					1246:1250	a total	1244:1250	a total of 11 different polymers	1244:1275	According to the results, the analyzed HCW was composed mainly of polypropylene (80.88%), high-density polyethylene (5.28%), polystyrene (4.51%), and cellulose (3.58%), from a total of 11 different polymers.
32238096	4	25	theme	different	1258:1266	arg1	polymers					1268:1275	11 different polymers	1255:1275	11 different polymers	1255:1275	According to the results, the analyzed HCW was composed mainly of polypropylene (80.88%), high-density polyethylene (5.28%), polystyrene (4.51%), and cellulose (3.58%), from a total of 11 different polymers.
32238096	3	26	theme	medical	729:735	arg1	devices					737:743	medical devices	729:743	medical devices	729:743	The proposed approach is composed of 4 steps: (1) Collecting data about consumed medical devices to be categorized into the residues Groups (A and E); (2) Identifying the polymeric composition with information provided by suppliers; (3) Characterizing the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR) and (4) Determining the polymer melting point by Differential Scanning Calorimetry (DSC).
32238096	2	27	theme	plastics	575:582	arg1	plastics					575:582	plastics	575:582	plastics	575:582	Among the waste produced from the medical devices, 3.14 ton (98.79%) were composed of polymers (63.06% of plastics and 35.73% elastomers) while around 0.03 ton (1.21%) by metals.
32238096	2	27	theme	plastics	575:582	arg1	%					593:593	35.73%	588:593	35.73%	588:593	Among the waste produced from the medical devices, 3.14 ton (98.79%) were composed of polymers (63.06% of plastics and 35.73% elastomers) while around 0.03 ton (1.21%) by metals.
32238096	2	27	theme	plastics	575:582	arg1	%					570:570	63.06%	565:570	63.06% of plastics	565:582	Among the waste produced from the medical devices, 3.14 ton (98.79%) were composed of polymers (63.06% of plastics and 35.73% elastomers) while around 0.03 ton (1.21%) by metals.
32238096	3	28	theme	polymer	1004:1010	arg1	point					1020:1024	the polymer melting point	1000:1024	the polymer melting point	1000:1024	The proposed approach is composed of 4 steps: (1) Collecting data about consumed medical devices to be categorized into the residues Groups (A and E); (2) Identifying the polymeric composition with information provided by suppliers; (3) Characterizing the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR) and (4) Determining the polymer melting point by Differential Scanning Calorimetry (DSC).
32238096	1	29	theme	medical	344:350	arg1	devices					352:358	medical devices	344:358	medical devices that are consumed during a year, discarded either as infecting (Group A) or as scarifying residue (Group E)	344:466	This work presents the health-care waste (HCW) management and an approach to assess and identify polymers in a General Surgery Unit - Internment Service (GSU) of a Brazilian university hospital, to estimate the main polymers presenting in medical devices that are consumed during a year, discarded either as infecting (Group A) or as scarifying residue (Group E).
32238096	1	30	theme	Group	424:428	arg1	A					430:430	Group A	424:430	Group A	424:430	This work presents the health-care waste (HCW) management and an approach to assess and identify polymers in a General Surgery Unit - Internment Service (GSU) of a Brazilian university hospital, to estimate the main polymers presenting in medical devices that are consumed during a year, discarded either as infecting (Group A) or as scarifying residue (Group E).
32238096	1	30	theme	Group	424:428	arg1	infecting					413:421	infecting	413:421	infecting	413:421	This work presents the health-care waste (HCW) management and an approach to assess and identify polymers in a General Surgery Unit - Internment Service (GSU) of a Brazilian university hospital, to estimate the main polymers presenting in medical devices that are consumed during a year, discarded either as infecting (Group A) or as scarifying residue (Group E).
32238096	3	31	theme	melting	1012:1018	arg1	point					1020:1024	the polymer melting point	1000:1024	the polymer melting point	1000:1024	The proposed approach is composed of 4 steps: (1) Collecting data about consumed medical devices to be categorized into the residues Groups (A and E); (2) Identifying the polymeric composition with information provided by suppliers; (3) Characterizing the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR) and (4) Determining the polymer melting point by Differential Scanning Calorimetry (DSC).
32238096	4	32	theme	analyzed	1100:1107	arg1	HCW					1109:1111	the analyzed HCW	1096:1111	the analyzed HCW	1096:1111	According to the results, the analyzed HCW was composed mainly of polypropylene (80.88%), high-density polyethylene (5.28%), polystyrene (4.51%), and cellulose (3.58%), from a total of 11 different polymers.
32238096	3	33	dep	consumed	720:727	arg1	Characterizing					885:898	Characterizing	885:898	Characterizing the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR)	885:978	The proposed approach is composed of 4 steps: (1) Collecting data about consumed medical devices to be categorized into the residues Groups (A and E); (2) Identifying the polymeric composition with information provided by suppliers; (3) Characterizing the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR) and (4) Determining the polymer melting point by Differential Scanning Calorimetry (DSC).
32238096	3	33	dep	consumed	720:727	arg1	Determining					988:998	Determining	988:998	(4) Determining the polymer melting point by Differential Scanning Calorimetry (DSC)	984:1067	The proposed approach is composed of 4 steps: (1) Collecting data about consumed medical devices to be categorized into the residues Groups (A and E); (2) Identifying the polymeric composition with information provided by suppliers; (3) Characterizing the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR) and (4) Determining the polymer melting point by Differential Scanning Calorimetry (DSC).
32238096	3	33	dep	consumed	720:727	arg1	3					882:882	3	882:882	3	882:882	The proposed approach is composed of 4 steps: (1) Collecting data about consumed medical devices to be categorized into the residues Groups (A and E); (2) Identifying the polymeric composition with information provided by suppliers; (3) Characterizing the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR) and (4) Determining the polymer melting point by Differential Scanning Calorimetry (DSC).
32238096	3	33	dep	consumed	720:727	arg1	2					800:800	2	800:800	2	800:800	The proposed approach is composed of 4 steps: (1) Collecting data about consumed medical devices to be categorized into the residues Groups (A and E); (2) Identifying the polymeric composition with information provided by suppliers; (3) Characterizing the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR) and (4) Determining the polymer melting point by Differential Scanning Calorimetry (DSC).
32238096	3	33	dep	consumed	720:727	arg1	Identifying					803:813	Identifying	803:813	Identifying the polymeric composition with information provided by suppliers	803:878	The proposed approach is composed of 4 steps: (1) Collecting data about consumed medical devices to be categorized into the residues Groups (A and E); (2) Identifying the polymeric composition with information provided by suppliers; (3) Characterizing the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR) and (4) Determining the polymer melting point by Differential Scanning Calorimetry (DSC).
32238096	3	34	theme	polymer	904:910	arg1	groups					923:928	the polymer functional groups	900:928	the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR)	900:978	The proposed approach is composed of 4 steps: (1) Collecting data about consumed medical devices to be categorized into the residues Groups (A and E); (2) Identifying the polymeric composition with information provided by suppliers; (3) Characterizing the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR) and (4) Determining the polymer melting point by Differential Scanning Calorimetry (DSC).
32238096	3	35	theme	functional	912:921	arg1	groups					923:928	the polymer functional groups	900:928	the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR)	900:978	The proposed approach is composed of 4 steps: (1) Collecting data about consumed medical devices to be categorized into the residues Groups (A and E); (2) Identifying the polymeric composition with information provided by suppliers; (3) Characterizing the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR) and (4) Determining the polymer melting point by Differential Scanning Calorimetry (DSC).
32238096	3	36	theme	residues	772:779	arg1	Groups					781:786	the residues Groups	768:786	the residues Groups	768:786	The proposed approach is composed of 4 steps: (1) Collecting data about consumed medical devices to be categorized into the residues Groups (A and E); (2) Identifying the polymeric composition with information provided by suppliers; (3) Characterizing the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR) and (4) Determining the polymer melting point by Differential Scanning Calorimetry (DSC).
32238096	2	37	dep	while	607:611	arg1	around					613:618	around	613:618	around	613:618	Among the waste produced from the medical devices, 3.14 ton (98.79%) were composed of polymers (63.06% of plastics and 35.73% elastomers) while around 0.03 ton (1.21%) by metals.
32238096	0	38	theme	health-care	51:61	arg1	waste					63:67	the health-care waste	47:67	the health-care waste of a Brazilian university hospital	47:102	An approach to assess and identify polymers in the health-care waste of a Brazilian university hospital.
32238096	3	39	with	composition	829:839	arg1	information					846:856	information	846:856	information provided by suppliers	846:878	The proposed approach is composed of 4 steps: (1) Collecting data about consumed medical devices to be categorized into the residues Groups (A and E); (2) Identifying the polymeric composition with information provided by suppliers; (3) Characterizing the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR) and (4) Determining the polymer melting point by Differential Scanning Calorimetry (DSC).
32238096	2	40	dep	polymers	555:562	arg1	plastics					575:582	plastics	575:582	plastics	575:582	Among the waste produced from the medical devices, 3.14 ton (98.79%) were composed of polymers (63.06% of plastics and 35.73% elastomers) while around 0.03 ton (1.21%) by metals.
32238096	2	40	dep	polymers	555:562	arg1	%					593:593	35.73%	588:593	35.73%	588:593	Among the waste produced from the medical devices, 3.14 ton (98.79%) were composed of polymers (63.06% of plastics and 35.73% elastomers) while around 0.03 ton (1.21%) by metals.
32238096	2	40	dep	polymers	555:562	arg1	%					570:570	63.06%	565:570	63.06% of plastics	565:582	Among the waste produced from the medical devices, 3.14 ton (98.79%) were composed of polymers (63.06% of plastics and 35.73% elastomers) while around 0.03 ton (1.21%) by metals.
32238096	3	41	theme	proposed	652:659	arg1	approach					661:668	The proposed approach	648:668	The proposed approach	648:668	The proposed approach is composed of 4 steps: (1) Collecting data about consumed medical devices to be categorized into the residues Groups (A and E); (2) Identifying the polymeric composition with information provided by suppliers; (3) Characterizing the polymer functional groups by Fourier-Transform Infrared Spectroscopy (FTIR) and (4) Determining the polymer melting point by Differential Scanning Calorimetry (DSC).
32238096	1	42	theme	Brazilian	269:277	arg1	hospital					290:297	a Brazilian university hospital	267:297	a Brazilian university hospital	267:297	This work presents the health-care waste (HCW) management and an approach to assess and identify polymers in a General Surgery Unit - Internment Service (GSU) of a Brazilian university hospital, to estimate the main polymers presenting in medical devices that are consumed during a year, discarded either as infecting (Group A) or as scarifying residue (Group E).
32238096	1	43	theme	scarifying	439:448	arg1	E					465:465	Group E	459:465	Group E	459:465	This work presents the health-care waste (HCW) management and an approach to assess and identify polymers in a General Surgery Unit - Internment Service (GSU) of a Brazilian university hospital, to estimate the main polymers presenting in medical devices that are consumed during a year, discarded either as infecting (Group A) or as scarifying residue (Group E).
32238096	1	43	theme	scarifying	439:448	arg1	residue					450:456	scarifying residue	439:456	scarifying residue (Group E)	439:466	This work presents the health-care waste (HCW) management and an approach to assess and identify polymers in a General Surgery Unit - Internment Service (GSU) of a Brazilian university hospital, to estimate the main polymers presenting in medical devices that are consumed during a year, discarded either as infecting (Group A) or as scarifying residue (Group E).
32238096	1	44	theme	health-care	128:138	arg1	management					152:161	the health-care waste (HCW) management	124:161	the health-care waste (HCW) management	124:161	This work presents the health-care waste (HCW) management and an approach to assess and identify polymers in a General Surgery Unit - Internment Service (GSU) of a Brazilian university hospital, to estimate the main polymers presenting in medical devices that are consumed during a year, discarded either as infecting (Group A) or as scarifying residue (Group E).
31876413	4	0	from	processes	669:677	arg1	ubiquity					617:624	the ubiquity	613:624	the ubiquity of bactoprenyl phosphate in these critical processes	613:677	Because of the ubiquity of bactoprenyl phosphate in these critical processes, molecular probes appended to this lipid carrier simplify identification of enzymatic roles during polysaccharide bioassembly.
31876413	4	1	theme	enzymatic	755:763	arg1	roles					765:769	enzymatic roles	755:769	enzymatic roles during polysaccharide bioassembly	755:803	Because of the ubiquity of bactoprenyl phosphate in these critical processes, molecular probes appended to this lipid carrier simplify identification of enzymatic roles during polysaccharide bioassembly.
31876413	4	2	theme	phosphate	641:649	arg1	ubiquity					617:624	the ubiquity	613:624	the ubiquity of bactoprenyl phosphate in these critical processes	613:677	Because of the ubiquity of bactoprenyl phosphate in these critical processes, molecular probes appended to this lipid carrier simplify identification of enzymatic roles during polysaccharide bioassembly.
31876413	8	3	dep	probes	1262:1267	arg1	utilizing					1269:1277	utilizing	1269:1277	utilizing four well-characterized initiating phosphoglycosyltransferases: CPS2E (Streptococcus pneumoniae), WbaP (Salmonella enterica), WecA (Escherichia coli), and WecP (Aeromonas hydrophilia)	1269:1461	We then assess the enzyme promiscuity of these two probes utilizing four well-characterized initiating phosphoglycosyltransferases: CPS2E (Streptococcus pneumoniae), WbaP (Salmonella enterica), WecA (Escherichia coli), and WecP (Aeromonas hydrophilia).
31876413	2	4	theme	tremendous	382:391	arg1	diversity					393:401	the tremendous diversity	378:401	the tremendous diversity in glycan composition among bacterial species	378:447	A major barrier to our fundamental understanding of these complex surface polysaccharides lies in the tremendous diversity in glycan composition among bacterial species.
31876413	10	5	theme	solubility	1651:1660	arg1	requirements					1662:1673	unique solubility requirements	1644:1673	unique solubility requirements for the nitrobenzoxadizol moiety for efficient enzymatic utilization that was not observed for the 2-nitrileaniline	1644:1789	Interestingly, we have also identified unique solubility requirements for the nitrobenzoxadizol moiety for efficient enzymatic utilization that was not observed for the 2-nitrileaniline.
31876413	4	6	theme	bactoprenyl	629:639	arg1	phosphate					641:649	bactoprenyl phosphate	629:649	bactoprenyl phosphate in these critical processes	629:677	Because of the ubiquity of bactoprenyl phosphate in these critical processes, molecular probes appended to this lipid carrier simplify identification of enzymatic roles during polysaccharide bioassembly.
31876413	5	7	theme	limited	808:814	arg1	number					816:821	A limited number	806:821	A limited number of these probes	806:837	A limited number of these probes exist in the literature or have been assessed with such pathways, and the limits of their use are not currently known.
31876413	10	8	theme	unique	1644:1649	arg1	requirements					1662:1673	unique solubility requirements	1644:1673	unique solubility requirements for the nitrobenzoxadizol moiety for efficient enzymatic utilization that was not observed for the 2-nitrileaniline	1644:1789	Interestingly, we have also identified unique solubility requirements for the nitrobenzoxadizol moiety for efficient enzymatic utilization that was not observed for the 2-nitrileaniline.
31876413	10	9	theme	enzymatic	1722:1730	arg1	utilization					1732:1742	efficient enzymatic utilization	1712:1742	efficient enzymatic utilization that was not observed for the 2-nitrileaniline	1712:1789	Interestingly, we have also identified unique solubility requirements for the nitrobenzoxadizol moiety for efficient enzymatic utilization that was not observed for the 2-nitrileaniline.
31876413	8	10	dep	WbaP	1377:1380	arg1	enterica					1394:1401	Salmonella enterica	1383:1401	Salmonella enterica	1383:1401	We then assess the enzyme promiscuity of these two probes utilizing four well-characterized initiating phosphoglycosyltransferases: CPS2E (Streptococcus pneumoniae), WbaP (Salmonella enterica), WecA (Escherichia coli), and WecP (Aeromonas hydrophilia).
31876413	9	11	theme	pathways	1595:1602	arg1	range					1562:1566	a wide range	1555:1566	a wide range of bacterial glycoassembly pathways	1555:1602	Both probes serve as substrates for these enzymes and could be readily used to investigate a wide range of bacterial glycoassembly pathways.
31876413	2	12	theme	fundamental	303:313	arg1	understanding					315:327	our fundamental understanding	299:327	our fundamental understanding of these complex surface polysaccharides	299:368	A major barrier to our fundamental understanding of these complex surface polysaccharides lies in the tremendous diversity in glycan composition among bacterial species.
31876413	3	13	theme	lipid	535:539	arg1	carrier					541:547	an essential lipid carrier	522:547	an essential lipid carrier necessary for early stages of glycopolymer assembly	522:599	The polyisoprenoid bactoprenyl phosphate (or undecaprenyl phosphate) is an essential lipid carrier necessary for early stages of glycopolymer assembly.
31876413	3	13	theme	lipid	535:539	arg1	phosphate					481:489	The polyisoprenoid bactoprenyl phosphate	450:489	The polyisoprenoid bactoprenyl phosphate (or undecaprenyl phosphate)	450:517	The polyisoprenoid bactoprenyl phosphate (or undecaprenyl phosphate) is an essential lipid carrier necessary for early stages of glycopolymer assembly.
31876413	4	14	theme	roles	765:769	arg1	identification					737:750	identification	737:750	identification of enzymatic roles during polysaccharide bioassembly	737:803	Because of the ubiquity of bactoprenyl phosphate in these critical processes, molecular probes appended to this lipid carrier simplify identification of enzymatic roles during polysaccharide bioassembly.
31876413	7	15	theme	first	1199:1203	arg1	time					1205:1208	the first time	1195:1208	the first time	1195:1208	We further expand our previous efforts utilizing 2-nitrileaniline and additionally prepare nitrobenzoxadizol-tagged bactoprenyl phosphate for the first time.
31876413	3	16	theme	glycopolymer	579:590	arg1	assembly					592:599	glycopolymer assembly	579:599	glycopolymer assembly	579:599	The polyisoprenoid bactoprenyl phosphate (or undecaprenyl phosphate) is an essential lipid carrier necessary for early stages of glycopolymer assembly.
31876413	8	17	theme	probes	1262:1267	arg1	promiscuity					1237:1247	the enzyme promiscuity	1226:1247	the enzyme promiscuity of these two probes utilizing four well-characterized initiating phosphoglycosyltransferases: CPS2E (Streptococcus pneumoniae), WbaP (Salmonella enterica), WecA (Escherichia coli), and WecP (Aeromonas hydrophilia)	1226:1461	We then assess the enzyme promiscuity of these two probes utilizing four well-characterized initiating phosphoglycosyltransferases: CPS2E (Streptococcus pneumoniae), WbaP (Salmonella enterica), WecA (Escherichia coli), and WecP (Aeromonas hydrophilia).
31876413	2	18	from	diversity	393:401	arg1	composition					413:423	glycan composition	406:423	glycan composition	406:423	A major barrier to our fundamental understanding of these complex surface polysaccharides lies in the tremendous diversity in glycan composition among bacterial species.
31876413	1	19	theme	protective	105:114	arg1	surfaces					116:123	The protective surfaces	101:123	The protective surfaces of bacteria	101:135	The protective surfaces of bacteria are comprised of polysaccharides and are involved in host invasion and colonization, host immune system evasion, and antibacterial resistance.
31876413	2	20	theme	bacterial	431:439	arg1	species					441:447	bacterial species	431:447	bacterial species	431:447	A major barrier to our fundamental understanding of these complex surface polysaccharides lies in the tremendous diversity in glycan composition among bacterial species.
31876413	8	21	theme	well-characterized	1284:1301	arg1	phosphoglycosyltransferases					1314:1340	four well-characterized initiating phosphoglycosyltransferases	1279:1340	four well-characterized initiating phosphoglycosyltransferases	1279:1340	We then assess the enzyme promiscuity of these two probes utilizing four well-characterized initiating phosphoglycosyltransferases: CPS2E (Streptococcus pneumoniae), WbaP (Salmonella enterica), WecA (Escherichia coli), and WecP (Aeromonas hydrophilia).
31876413	4	22	theme	lipid	714:718	arg1	carrier					720:726	this lipid carrier	709:726	this lipid carrier	709:726	Because of the ubiquity of bactoprenyl phosphate in these critical processes, molecular probes appended to this lipid carrier simplify identification of enzymatic roles during polysaccharide bioassembly.
31876413	2	23	theme	surface	346:352	arg1	polysaccharides					354:368	these complex surface polysaccharides	332:368	these complex surface polysaccharides	332:368	A major barrier to our fundamental understanding of these complex surface polysaccharides lies in the tremendous diversity in glycan composition among bacterial species.
31876413	5	24	theme	use	929:931	arg1	limits					913:918	the limits	909:918	the limits of their use	909:931	A limited number of these probes exist in the literature or have been assessed with such pathways, and the limits of their use are not currently known.
31876413	0	25	theme	General	0:6	arg1	Utilization					8:18	General Utilization	0:18	General Utilization of Fluorescent Polyisoprenoids with Sugar Selective Phosphoglycosyltransferases.	0:99	General Utilization of Fluorescent Polyisoprenoids with Sugar Selective Phosphoglycosyltransferases.
31876413	6	26	theme	bactoprenyl	1033:1043	arg1	probes					1045:1050	fluorescently modified bactoprenyl probes	1010:1050	fluorescently modified bactoprenyl probes	1010:1050	Herein, we devise an efficient method for producing fluorescently modified bactoprenyl probes.
31876413	2	27	theme	complex	338:344	arg1	polysaccharides					354:368	these complex surface polysaccharides	332:368	these complex surface polysaccharides	332:368	A major barrier to our fundamental understanding of these complex surface polysaccharides lies in the tremendous diversity in glycan composition among bacterial species.
31876413	0	28	theme	Fluorescent	23:33	arg1	Polyisoprenoids					35:49	Fluorescent Polyisoprenoids	23:49	Fluorescent Polyisoprenoids	23:49	General Utilization of Fluorescent Polyisoprenoids with Sugar Selective Phosphoglycosyltransferases.
31876413	4	29	from	ubiquity	617:624	arg1	processes					669:677	these critical processes	654:677	these critical processes	654:677	Because of the ubiquity of bactoprenyl phosphate in these critical processes, molecular probes appended to this lipid carrier simplify identification of enzymatic roles during polysaccharide bioassembly.
31876413	7	30	theme	bactoprenyl	1169:1179	arg1	phosphate					1181:1189	nitrobenzoxadizol-tagged bactoprenyl phosphate	1144:1189	nitrobenzoxadizol-tagged bactoprenyl phosphate	1144:1189	We further expand our previous efforts utilizing 2-nitrileaniline and additionally prepare nitrobenzoxadizol-tagged bactoprenyl phosphate for the first time.
31876413	1	31	theme	bacteria	128:135	arg1	surfaces					116:123	The protective surfaces	101:123	The protective surfaces of bacteria	101:135	The protective surfaces of bacteria are comprised of polysaccharides and are involved in host invasion and colonization, host immune system evasion, and antibacterial resistance.
31876413	9	32	used	used	1535:1538	arg2	probes					1469:1474	Both probes	1464:1474	Both probes	1464:1474	Both probes serve as substrates for these enzymes and could be readily used to investigate a wide range of bacterial glycoassembly pathways.
31876413	9	32	used	used	1535:1538	arg2	substrates					1485:1494	substrates	1485:1494	substrates for these enzymes	1485:1512	Both probes serve as substrates for these enzymes and could be readily used to investigate a wide range of bacterial glycoassembly pathways.
31876413	1	33	theme	host	222:225	arg1	evasion					241:247	host immune system evasion	222:247	host immune system evasion	222:247	The protective surfaces of bacteria are comprised of polysaccharides and are involved in host invasion and colonization, host immune system evasion, and antibacterial resistance.
31876413	8	34	theme	enzyme	1230:1235	arg1	promiscuity					1237:1247	the enzyme promiscuity	1226:1247	the enzyme promiscuity of these two probes utilizing four well-characterized initiating phosphoglycosyltransferases: CPS2E (Streptococcus pneumoniae), WbaP (Salmonella enterica), WecA (Escherichia coli), and WecP (Aeromonas hydrophilia)	1226:1461	We then assess the enzyme promiscuity of these two probes utilizing four well-characterized initiating phosphoglycosyltransferases: CPS2E (Streptococcus pneumoniae), WbaP (Salmonella enterica), WecA (Escherichia coli), and WecP (Aeromonas hydrophilia).
31876413	9	35	theme	glycoassembly	1581:1593	arg1	pathways					1595:1602	bacterial glycoassembly pathways	1571:1602	bacterial glycoassembly pathways	1571:1602	Both probes serve as substrates for these enzymes and could be readily used to investigate a wide range of bacterial glycoassembly pathways.
31876413	1	36	theme	host	190:193	arg1	invasion					195:202	host invasion	190:202	host invasion	190:202	The protective surfaces of bacteria are comprised of polysaccharides and are involved in host invasion and colonization, host immune system evasion, and antibacterial resistance.
31876413	1	37	theme	immune	227:232	arg1	evasion					241:247	host immune system evasion	222:247	host immune system evasion	222:247	The protective surfaces of bacteria are comprised of polysaccharides and are involved in host invasion and colonization, host immune system evasion, and antibacterial resistance.
31876413	3	38	theme	polyisoprenoid	454:467	arg1	carrier					541:547	an essential lipid carrier	522:547	an essential lipid carrier necessary for early stages of glycopolymer assembly	522:599	The polyisoprenoid bactoprenyl phosphate (or undecaprenyl phosphate) is an essential lipid carrier necessary for early stages of glycopolymer assembly.
31876413	3	38	theme	polyisoprenoid	454:467	arg1	phosphate					481:489	The polyisoprenoid bactoprenyl phosphate	450:489	The polyisoprenoid bactoprenyl phosphate (or undecaprenyl phosphate)	450:517	The polyisoprenoid bactoprenyl phosphate (or undecaprenyl phosphate) is an essential lipid carrier necessary for early stages of glycopolymer assembly.
31876413	3	38	theme	polyisoprenoid	454:467	arg1	phosphate					508:516	undecaprenyl phosphate	495:516	undecaprenyl phosphate	495:516	The polyisoprenoid bactoprenyl phosphate (or undecaprenyl phosphate) is an essential lipid carrier necessary for early stages of glycopolymer assembly.
31876413	4	39	theme	polysaccharide	778:791	arg1	bioassembly					793:803	polysaccharide bioassembly	778:803	polysaccharide bioassembly	778:803	Because of the ubiquity of bactoprenyl phosphate in these critical processes, molecular probes appended to this lipid carrier simplify identification of enzymatic roles during polysaccharide bioassembly.
31876413	8	40	dep	CPS2E	1343:1347	arg1	pneumoniae					1364:1373	Streptococcus pneumoniae	1350:1373	Streptococcus pneumoniae	1350:1373	We then assess the enzyme promiscuity of these two probes utilizing four well-characterized initiating phosphoglycosyltransferases: CPS2E (Streptococcus pneumoniae), WbaP (Salmonella enterica), WecA (Escherichia coli), and WecP (Aeromonas hydrophilia).
31876413	9	41	theme	wide	1557:1560	arg1	range					1562:1566	a wide range	1555:1566	a wide range of bacterial glycoassembly pathways	1555:1602	Both probes serve as substrates for these enzymes and could be readily used to investigate a wide range of bacterial glycoassembly pathways.
31876413	1	42	theme	system	234:239	arg1	evasion					241:247	host immune system evasion	222:247	host immune system evasion	222:247	The protective surfaces of bacteria are comprised of polysaccharides and are involved in host invasion and colonization, host immune system evasion, and antibacterial resistance.
31876413	0	43	theme	Polyisoprenoids	35:49	arg1	Utilization					8:18	General Utilization	0:18	General Utilization of Fluorescent Polyisoprenoids with Sugar Selective Phosphoglycosyltransferases.	0:99	General Utilization of Fluorescent Polyisoprenoids with Sugar Selective Phosphoglycosyltransferases.
31876413	3	44	theme	bactoprenyl	469:479	arg1	carrier					541:547	an essential lipid carrier	522:547	an essential lipid carrier necessary for early stages of glycopolymer assembly	522:599	The polyisoprenoid bactoprenyl phosphate (or undecaprenyl phosphate) is an essential lipid carrier necessary for early stages of glycopolymer assembly.
31876413	3	44	theme	bactoprenyl	469:479	arg1	phosphate					481:489	The polyisoprenoid bactoprenyl phosphate	450:489	The polyisoprenoid bactoprenyl phosphate (or undecaprenyl phosphate)	450:517	The polyisoprenoid bactoprenyl phosphate (or undecaprenyl phosphate) is an essential lipid carrier necessary for early stages of glycopolymer assembly.
31876413	3	44	theme	bactoprenyl	469:479	arg1	phosphate					508:516	undecaprenyl phosphate	495:516	undecaprenyl phosphate	495:516	The polyisoprenoid bactoprenyl phosphate (or undecaprenyl phosphate) is an essential lipid carrier necessary for early stages of glycopolymer assembly.
31876413	9	45	theme	bacterial	1571:1579	arg1	pathways					1595:1602	bacterial glycoassembly pathways	1571:1602	bacterial glycoassembly pathways	1571:1602	Both probes serve as substrates for these enzymes and could be readily used to investigate a wide range of bacterial glycoassembly pathways.
31876413	4	46	theme	molecular	680:688	arg1	probes					690:695	molecular probes	680:695	molecular probes appended to this lipid carrier	680:726	Because of the ubiquity of bactoprenyl phosphate in these critical processes, molecular probes appended to this lipid carrier simplify identification of enzymatic roles during polysaccharide bioassembly.
31876413	7	47	theme	previous	1075:1082	arg1	efforts					1084:1090	our previous efforts	1071:1090	our previous efforts utilizing 2-nitrileaniline	1071:1117	We further expand our previous efforts utilizing 2-nitrileaniline and additionally prepare nitrobenzoxadizol-tagged bactoprenyl phosphate for the first time.
31876413	5	48	theme	such	890:893	arg1	pathways					895:902	such pathways	890:902	such pathways	890:902	A limited number of these probes exist in the literature or have been assessed with such pathways, and the limits of their use are not currently known.
31876413	0	49	theme	Selective	62:70	arg1	Phosphoglycosyltransferases					72:98	Sugar Selective Phosphoglycosyltransferases	56:98	Sugar Selective Phosphoglycosyltransferases	56:98	General Utilization of Fluorescent Polyisoprenoids with Sugar Selective Phosphoglycosyltransferases.
31876413	6	50	theme	modified	1024:1031	arg1	probes					1045:1050	fluorescently modified bactoprenyl probes	1010:1050	fluorescently modified bactoprenyl probes	1010:1050	Herein, we devise an efficient method for producing fluorescently modified bactoprenyl probes.
31876413	7	51	theme	nitrobenzoxadizol-tagged	1144:1167	arg1	phosphate					1181:1189	nitrobenzoxadizol-tagged bactoprenyl phosphate	1144:1189	nitrobenzoxadizol-tagged bactoprenyl phosphate	1144:1189	We further expand our previous efforts utilizing 2-nitrileaniline and additionally prepare nitrobenzoxadizol-tagged bactoprenyl phosphate for the first time.
31876413	0	52	theme	Sugar	56:60	arg1	Phosphoglycosyltransferases					72:98	Sugar Selective Phosphoglycosyltransferases	56:98	Sugar Selective Phosphoglycosyltransferases	56:98	General Utilization of Fluorescent Polyisoprenoids with Sugar Selective Phosphoglycosyltransferases.
31876413	6	53	theme	efficient	979:987	arg1	method					989:994	an efficient method	976:994	an efficient method for producing fluorescently modified bactoprenyl probes	976:1050	Herein, we devise an efficient method for producing fluorescently modified bactoprenyl probes.
31876413	0	54	with	Utilization	8:18	arg1	Phosphoglycosyltransferases					72:98	Sugar Selective Phosphoglycosyltransferases	56:98	Sugar Selective Phosphoglycosyltransferases	56:98	General Utilization of Fluorescent Polyisoprenoids with Sugar Selective Phosphoglycosyltransferases.
31876413	3	55	theme	necessary	549:557	arg1	carrier					541:547	an essential lipid carrier	522:547	an essential lipid carrier necessary for early stages of glycopolymer assembly	522:599	The polyisoprenoid bactoprenyl phosphate (or undecaprenyl phosphate) is an essential lipid carrier necessary for early stages of glycopolymer assembly.
31876413	3	55	theme	necessary	549:557	arg1	phosphate					481:489	The polyisoprenoid bactoprenyl phosphate	450:489	The polyisoprenoid bactoprenyl phosphate (or undecaprenyl phosphate)	450:517	The polyisoprenoid bactoprenyl phosphate (or undecaprenyl phosphate) is an essential lipid carrier necessary for early stages of glycopolymer assembly.
31876413	3	56	theme	early	563:567	arg1	stages					569:574	early stages	563:574	early stages of glycopolymer assembly	563:599	The polyisoprenoid bactoprenyl phosphate (or undecaprenyl phosphate) is an essential lipid carrier necessary for early stages of glycopolymer assembly.
31876413	3	57	theme	essential	525:533	arg1	carrier					541:547	an essential lipid carrier	522:547	an essential lipid carrier necessary for early stages of glycopolymer assembly	522:599	The polyisoprenoid bactoprenyl phosphate (or undecaprenyl phosphate) is an essential lipid carrier necessary for early stages of glycopolymer assembly.
31876413	3	57	theme	essential	525:533	arg1	phosphate					481:489	The polyisoprenoid bactoprenyl phosphate	450:489	The polyisoprenoid bactoprenyl phosphate (or undecaprenyl phosphate)	450:517	The polyisoprenoid bactoprenyl phosphate (or undecaprenyl phosphate) is an essential lipid carrier necessary for early stages of glycopolymer assembly.
31876413	4	58	from	phosphate	641:649	arg1	processes					669:677	these critical processes	654:677	these critical processes	654:677	Because of the ubiquity of bactoprenyl phosphate in these critical processes, molecular probes appended to this lipid carrier simplify identification of enzymatic roles during polysaccharide bioassembly.
31876413	3	59	theme	assembly	592:599	arg1	stages					569:574	early stages	563:574	early stages of glycopolymer assembly	563:599	The polyisoprenoid bactoprenyl phosphate (or undecaprenyl phosphate) is an essential lipid carrier necessary for early stages of glycopolymer assembly.
31876413	2	60	theme	major	282:286	arg1	barrier					288:294	A major barrier	280:294	A major barrier to our fundamental understanding of these complex surface polysaccharides	280:368	A major barrier to our fundamental understanding of these complex surface polysaccharides lies in the tremendous diversity in glycan composition among bacterial species.
31876413	2	61	theme	polysaccharides	354:368	arg1	understanding					315:327	our fundamental understanding	299:327	our fundamental understanding of these complex surface polysaccharides	299:368	A major barrier to our fundamental understanding of these complex surface polysaccharides lies in the tremendous diversity in glycan composition among bacterial species.
31876413	4	62	theme	critical	660:667	arg1	processes					669:677	these critical processes	654:677	these critical processes	654:677	Because of the ubiquity of bactoprenyl phosphate in these critical processes, molecular probes appended to this lipid carrier simplify identification of enzymatic roles during polysaccharide bioassembly.
31876413	5	63	theme	probes	832:837	arg1	number					816:821	A limited number	806:821	A limited number of these probes	806:837	A limited number of these probes exist in the literature or have been assessed with such pathways, and the limits of their use are not currently known.
31876413	8	64	dep	WecP	1434:1437	arg1	hydrophilia					1450:1460	Aeromonas hydrophilia	1440:1460	Aeromonas hydrophilia	1440:1460	We then assess the enzyme promiscuity of these two probes utilizing four well-characterized initiating phosphoglycosyltransferases: CPS2E (Streptococcus pneumoniae), WbaP (Salmonella enterica), WecA (Escherichia coli), and WecP (Aeromonas hydrophilia).
31876413	8	65	dep	WecA	1405:1408	arg1	coli					1423:1426	Escherichia coli	1411:1426	Escherichia coli	1411:1426	We then assess the enzyme promiscuity of these two probes utilizing four well-characterized initiating phosphoglycosyltransferases: CPS2E (Streptococcus pneumoniae), WbaP (Salmonella enterica), WecA (Escherichia coli), and WecP (Aeromonas hydrophilia).
31876413	2	66	theme	glycan	406:411	arg1	composition					413:423	glycan composition	406:423	glycan composition	406:423	A major barrier to our fundamental understanding of these complex surface polysaccharides lies in the tremendous diversity in glycan composition among bacterial species.
31876413	3	67	theme	undecaprenyl	495:506	arg1	phosphate					481:489	The polyisoprenoid bactoprenyl phosphate	450:489	The polyisoprenoid bactoprenyl phosphate (or undecaprenyl phosphate)	450:517	The polyisoprenoid bactoprenyl phosphate (or undecaprenyl phosphate) is an essential lipid carrier necessary for early stages of glycopolymer assembly.
31876413	3	67	theme	undecaprenyl	495:506	arg1	phosphate					508:516	undecaprenyl phosphate	495:516	undecaprenyl phosphate	495:516	The polyisoprenoid bactoprenyl phosphate (or undecaprenyl phosphate) is an essential lipid carrier necessary for early stages of glycopolymer assembly.
31876413	1	68	theme	antibacterial	254:266	arg1	resistance					268:277	antibacterial resistance	254:277	antibacterial resistance	254:277	The protective surfaces of bacteria are comprised of polysaccharides and are involved in host invasion and colonization, host immune system evasion, and antibacterial resistance.
31876413	8	69	theme	initiating	1303:1312	arg1	phosphoglycosyltransferases					1314:1340	four well-characterized initiating phosphoglycosyltransferases	1279:1340	four well-characterized initiating phosphoglycosyltransferases	1279:1340	We then assess the enzyme promiscuity of these two probes utilizing four well-characterized initiating phosphoglycosyltransferases: CPS2E (Streptococcus pneumoniae), WbaP (Salmonella enterica), WecA (Escherichia coli), and WecP (Aeromonas hydrophilia).
31876413	10	70	theme	nitrobenzoxadizol	1683:1699	arg1	moiety					1701:1706	the nitrobenzoxadizol moiety	1679:1706	the nitrobenzoxadizol moiety	1679:1706	Interestingly, we have also identified unique solubility requirements for the nitrobenzoxadizol moiety for efficient enzymatic utilization that was not observed for the 2-nitrileaniline.
31876413	10	71	theme	efficient	1712:1720	arg1	utilization					1732:1742	efficient enzymatic utilization	1712:1742	efficient enzymatic utilization that was not observed for the 2-nitrileaniline	1712:1789	Interestingly, we have also identified unique solubility requirements for the nitrobenzoxadizol moiety for efficient enzymatic utilization that was not observed for the 2-nitrileaniline.
33691970	4	0	theme	novel	474:478	arg1	alleles					480:486	five novel alleles	469:486	five novel alleles for the Wx-A1 gene	469:505	Within these materials, five novel alleles for the Wx-A1 gene were detected.
33691970	7	1	theme	Class	912:916	arg1	transposon					921:930	a Class II transposon	910:930	a Class II transposon of the Mutator superfamily, which had not been described previously, and has been named Baetica	910:1026	This insert exhibited the characteristics of a Class II transposon of the Mutator superfamily, which had not been described previously, and has been named Baetica.
33691970	9	2	theme	landraces	1249:1257	arg1	evaluation					1225:1234	the evaluation	1221:1234	the evaluation of old wheat landraces	1221:1257	In conclusion, the evaluation of old wheat landraces showed that, in addition to their use as alternative crops, these materials could be a useful source of interesting genes in wheat quality improvement.
33691970	0	3	from	characterization	10:25	arg1	wheat					65:69	common wheat	58:69	common wheat	58:69	Molecular characterization of five novel Wx-A1 alleles in common wheat including one silent allele by transposon insertion.
33691970	6	4	theme	protein	733:739	arg1	loss					715:718	loss	715:718	loss of the Wx-A1 protein	715:739	However, one of them (Wx-A1t) exhibited loss of the Wx-A1 protein, and its base sequence contained one large insert (1,073 bp) in the tenth exon, that interrupted the ORF of the Wx-A1 gene.
33691970	9	5	theme	wheat	1243:1247	arg1	landraces					1249:1257	old wheat landraces	1239:1257	old wheat landraces	1239:1257	In conclusion, the evaluation of old wheat landraces showed that, in addition to their use as alternative crops, these materials could be a useful source of interesting genes in wheat quality improvement.
33691970	3	6	theme	protein	341:347	arg1	composition					349:359	The waxy protein composition	332:359	The waxy protein composition of 45 Spanish common wheat landraces from Andalusia (southern Spain)	332:428	The waxy protein composition of 45 Spanish common wheat landraces from Andalusia (southern Spain) was evaluated.
33691970	3	7	from	Andalusia	403:411	arg1	landraces					388:396	45 Spanish common wheat landraces	364:396	45 Spanish common wheat landraces from Andalusia (southern Spain)	364:428	The waxy protein composition of 45 Spanish common wheat landraces from Andalusia (southern Spain) was evaluated.
33691970	3	7	from	Andalusia	403:411	arg1	composition					349:359	The waxy protein composition	332:359	The waxy protein composition of 45 Spanish common wheat landraces from Andalusia (southern Spain)	332:428	The waxy protein composition of 45 Spanish common wheat landraces from Andalusia (southern Spain) was evaluated.
33691970	6	8	theme	Wx-A1	727:731	arg1	protein					733:739	the Wx-A1 protein	723:739	the Wx-A1 protein	723:739	However, one of them (Wx-A1t) exhibited loss of the Wx-A1 protein, and its base sequence contained one large insert (1,073 bp) in the tenth exon, that interrupted the ORF of the Wx-A1 gene.
33691970	2	9	theme	waxy	277:280	arg1	protein					282:288	only the waxy protein	268:288	only the waxy protein	268:288	Although several starch synthases are responsible for its synthesis, only the waxy protein is associated with the amylose synthesis.
33691970	9	10	theme	quality	1390:1396	arg1	improvement					1398:1408	wheat quality improvement	1384:1408	wheat quality improvement	1384:1408	In conclusion, the evaluation of old wheat landraces showed that, in addition to their use as alternative crops, these materials could be a useful source of interesting genes in wheat quality improvement.
33691970	6	11	theme	gene	859:862	arg1	ORF					842:844	the ORF	838:844	the ORF of the Wx-A1 gene	838:862	However, one of them (Wx-A1t) exhibited loss of the Wx-A1 protein, and its base sequence contained one large insert (1,073 bp) in the tenth exon, that interrupted the ORF of the Wx-A1 gene.
33691970	0	12	theme	silent	85:90	arg1	allele					92:97	one silent allele	81:97	one silent allele by transposon insertion	81:121	Molecular characterization of five novel Wx-A1 alleles in common wheat including one silent allele by transposon insertion.
33691970	5	13	theme	amino	613:617	arg1	changes					624:630	some amino acid changes	608:630	some amino acid changes	608:630	Four of them showed functional proteins (Wx-A1p, Wx-A1q, Wx-A1r and Wx-A1s), although some amino acid changes were found in the mature protein sequence.
33691970	6	14	theme	Wx-A1	853:857	arg1	gene					859:862	the Wx-A1 gene	849:862	the Wx-A1 gene	849:862	However, one of them (Wx-A1t) exhibited loss of the Wx-A1 protein, and its base sequence contained one large insert (1,073 bp) in the tenth exon, that interrupted the ORF of the Wx-A1 gene.
33691970	0	15	theme	transposon	102:111	arg1	insertion					113:121	transposon insertion	102:121	transposon insertion	102:121	Molecular characterization of five novel Wx-A1 alleles in common wheat including one silent allele by transposon insertion.
33691970	8	16	theme	technological	1183:1195	arg1	quality					1197:1203	technological quality	1183:1203	technological quality	1183:1203	The conservation of such inserts could be related to their low effect on vital properties of the plants, as occurs with most of the genes associated with technological quality.
33691970	1	17	theme	glucose	156:162	arg1	amylopectin					186:196	amylopectin	186:196	amylopectin	186:196	Wheat starch is composed of two glucose polymers, amylose and amylopectin.
33691970	1	17	theme	glucose	156:162	arg1	amylose					174:180	amylose	174:180	amylose	174:180	Wheat starch is composed of two glucose polymers, amylose and amylopectin.
33691970	1	17	theme	glucose	156:162	arg1	polymers					164:171	two glucose polymers	152:171	two glucose polymers	152:171	Wheat starch is composed of two glucose polymers, amylose and amylopectin.
33691970	3	18	theme	waxy	336:339	arg1	composition					349:359	The waxy protein composition	332:359	The waxy protein composition of 45 Spanish common wheat landraces from Andalusia (southern Spain)	332:428	The waxy protein composition of 45 Spanish common wheat landraces from Andalusia (southern Spain) was evaluated.
33691970	9	19	theme	old	1239:1241	arg1	landraces					1249:1257	old wheat landraces	1239:1257	old wheat landraces	1239:1257	In conclusion, the evaluation of old wheat landraces showed that, in addition to their use as alternative crops, these materials could be a useful source of interesting genes in wheat quality improvement.
33691970	0	20	theme	Molecular	0:8	arg1	characterization					10:25	Molecular characterization	0:25	Molecular characterization of five novel Wx-A1 alleles in common wheat	0:69	Molecular characterization of five novel Wx-A1 alleles in common wheat including one silent allele by transposon insertion.
33691970	3	21	theme	Spanish	367:373	arg1	landraces					388:396	45 Spanish common wheat landraces	364:396	45 Spanish common wheat landraces from Andalusia (southern Spain)	364:428	The waxy protein composition of 45 Spanish common wheat landraces from Andalusia (southern Spain) was evaluated.
33691970	7	22	theme	superfamily	947:957	arg1	transposon					921:930	a Class II transposon	910:930	a Class II transposon of the Mutator superfamily, which had not been described previously, and has been named Baetica	910:1026	This insert exhibited the characteristics of a Class II transposon of the Mutator superfamily, which had not been described previously, and has been named Baetica.
33691970	5	23	theme	functional	542:551	arg1	proteins					553:560	functional proteins	542:560	functional proteins (Wx-A1p, Wx-A1q, Wx-A1r and Wx-A1s)	542:596	Four of them showed functional proteins (Wx-A1p, Wx-A1q, Wx-A1r and Wx-A1s), although some amino acid changes were found in the mature protein sequence.
33691970	5	23	theme	functional	542:551	arg1	Wx-A1s					590:595	Wx-A1s	590:595	Wx-A1s	590:595	Four of them showed functional proteins (Wx-A1p, Wx-A1q, Wx-A1r and Wx-A1s), although some amino acid changes were found in the mature protein sequence.
33691970	5	23	theme	functional	542:551	arg1	Wx-A1r					579:584	Wx-A1r	579:584	Wx-A1r	579:584	Four of them showed functional proteins (Wx-A1p, Wx-A1q, Wx-A1r and Wx-A1s), although some amino acid changes were found in the mature protein sequence.
33691970	5	23	theme	functional	542:551	arg1	Wx-A1q					571:576	Wx-A1q	571:576	Wx-A1q	571:576	Four of them showed functional proteins (Wx-A1p, Wx-A1q, Wx-A1r and Wx-A1s), although some amino acid changes were found in the mature protein sequence.
33691970	5	23	theme	functional	542:551	arg1	Wx-A1p					563:568	Wx-A1p	563:568	Wx-A1p	563:568	Four of them showed functional proteins (Wx-A1p, Wx-A1q, Wx-A1r and Wx-A1s), although some amino acid changes were found in the mature protein sequence.
33691970	9	24	theme	genes	1375:1379	arg1	source					1353:1358	a useful source	1344:1358	a useful source of interesting genes in wheat quality improvement	1344:1408	In conclusion, the evaluation of old wheat landraces showed that, in addition to their use as alternative crops, these materials could be a useful source of interesting genes in wheat quality improvement.
33691970	9	24	theme	genes	1375:1379	arg1	materials					1325:1333	these materials	1319:1333	these materials	1319:1333	In conclusion, the evaluation of old wheat landraces showed that, in addition to their use as alternative crops, these materials could be a useful source of interesting genes in wheat quality improvement.
33691970	3	25	theme	common	375:380	arg1	landraces					388:396	45 Spanish common wheat landraces	364:396	45 Spanish common wheat landraces from Andalusia (southern Spain)	364:428	The waxy protein composition of 45 Spanish common wheat landraces from Andalusia (southern Spain) was evaluated.
33691970	8	26	theme	low	1088:1090	arg1	effect					1092:1097	their low effect	1082:1097	their low effect on vital properties of the plants	1082:1131	The conservation of such inserts could be related to their low effect on vital properties of the plants, as occurs with most of the genes associated with technological quality.
33691970	0	27	theme	alleles	47:53	arg1	characterization					10:25	Molecular characterization	0:25	Molecular characterization of five novel Wx-A1 alleles in common wheat	0:69	Molecular characterization of five novel Wx-A1 alleles in common wheat including one silent allele by transposon insertion.
33691970	3	28	theme	southern	414:421	arg1	Spain					423:427	southern Spain	414:427	southern Spain	414:427	The waxy protein composition of 45 Spanish common wheat landraces from Andalusia (southern Spain) was evaluated.
33691970	3	28	theme	southern	414:421	arg1	Andalusia					403:411	Andalusia	403:411	Andalusia (southern Spain)	403:428	The waxy protein composition of 45 Spanish common wheat landraces from Andalusia (southern Spain) was evaluated.
33691970	8	29	theme	inserts	1054:1060	arg1	conservation					1033:1044	The conservation	1029:1044	The conservation of such inserts	1029:1060	The conservation of such inserts could be related to their low effect on vital properties of the plants, as occurs with most of the genes associated with technological quality.
33691970	8	29	theme	inserts	1054:1060	arg1	related					1071:1077	related	1071:1077	related	1071:1077	The conservation of such inserts could be related to their low effect on vital properties of the plants, as occurs with most of the genes associated with technological quality.
33691970	9	30	theme	wheat	1384:1388	arg1	improvement					1398:1408	wheat quality improvement	1384:1408	wheat quality improvement	1384:1408	In conclusion, the evaluation of old wheat landraces showed that, in addition to their use as alternative crops, these materials could be a useful source of interesting genes in wheat quality improvement.
33691970	8	31	theme	such	1049:1052	arg1	inserts					1054:1060	such inserts	1049:1060	such inserts	1049:1060	The conservation of such inserts could be related to their low effect on vital properties of the plants, as occurs with most of the genes associated with technological quality.
33691970	7	32	theme	transposon	921:930	arg1	characteristics					891:905	the characteristics	887:905	the characteristics of a Class II transposon of the Mutator superfamily, which had not been described previously, and has been named Baetica	887:1026	This insert exhibited the characteristics of a Class II transposon of the Mutator superfamily, which had not been described previously, and has been named Baetica.
33691970	7	33	theme	Mutator	939:945	arg1	superfamily					947:957	the Mutator superfamily	935:957	the Mutator superfamily	935:957	This insert exhibited the characteristics of a Class II transposon of the Mutator superfamily, which had not been described previously, and has been named Baetica.
33691970	6	34	theme	large	778:782	arg1	insert					784:789	one large insert	774:789	one large insert (1,073 bp) in the tenth exon, that interrupted the ORF of the Wx-A1 gene	774:862	However, one of them (Wx-A1t) exhibited loss of the Wx-A1 protein, and its base sequence contained one large insert (1,073 bp) in the tenth exon, that interrupted the ORF of the Wx-A1 gene.
33691970	6	34	theme	large	778:782	arg1	bp					798:799	1,073 bp	792:799	1,073 bp	792:799	However, one of them (Wx-A1t) exhibited loss of the Wx-A1 protein, and its base sequence contained one large insert (1,073 bp) in the tenth exon, that interrupted the ORF of the Wx-A1 gene.
33691970	4	35	located	detected	512:519	arg2	alleles					480:486	five novel alleles	469:486	five novel alleles for the Wx-A1 gene	469:505	Within these materials, five novel alleles for the Wx-A1 gene were detected.
33691970	4	35	located	detected	512:519	arg1	materials					458:466	these materials	452:466	these materials	452:466	Within these materials, five novel alleles for the Wx-A1 gene were detected.
33691970	5	36	located	found	637:641	arg1	sequence					665:672	the mature protein sequence	646:672	the mature protein sequence	646:672	Four of them showed functional proteins (Wx-A1p, Wx-A1q, Wx-A1r and Wx-A1s), although some amino acid changes were found in the mature protein sequence.
33691970	5	36	located	found	637:641	arg2	changes					624:630	some amino acid changes	608:630	some amino acid changes	608:630	Four of them showed functional proteins (Wx-A1p, Wx-A1q, Wx-A1r and Wx-A1s), although some amino acid changes were found in the mature protein sequence.
33691970	8	37	theme	vital	1102:1106	arg1	properties					1108:1117	vital properties	1102:1117	vital properties of the plants	1102:1131	The conservation of such inserts could be related to their low effect on vital properties of the plants, as occurs with most of the genes associated with technological quality.
33691970	0	38	theme	Wx-A1	41:45	arg1	alleles					47:53	five novel Wx-A1 alleles	30:53	five novel Wx-A1 alleles	30:53	Molecular characterization of five novel Wx-A1 alleles in common wheat including one silent allele by transposon insertion.
33691970	8	39	theme	plants	1126:1131	arg1	properties					1108:1117	vital properties	1102:1117	vital properties of the plants	1102:1131	The conservation of such inserts could be related to their low effect on vital properties of the plants, as occurs with most of the genes associated with technological quality.
33691970	5	40	theme	mature	650:655	arg1	sequence					665:672	the mature protein sequence	646:672	the mature protein sequence	646:672	Four of them showed functional proteins (Wx-A1p, Wx-A1q, Wx-A1r and Wx-A1s), although some amino acid changes were found in the mature protein sequence.
33691970	0	41	theme	novel	35:39	arg1	alleles					47:53	five novel Wx-A1 alleles	30:53	five novel Wx-A1 alleles	30:53	Molecular characterization of five novel Wx-A1 alleles in common wheat including one silent allele by transposon insertion.
33691970	8	42	from	effect	1092:1097	arg1	properties					1108:1117	vital properties	1102:1117	vital properties of the plants	1102:1131	The conservation of such inserts could be related to their low effect on vital properties of the plants, as occurs with most of the genes associated with technological quality.
33691970	5	43	theme	protein	657:663	arg1	sequence					665:672	the mature protein sequence	646:672	the mature protein sequence	646:672	Four of them showed functional proteins (Wx-A1p, Wx-A1q, Wx-A1r and Wx-A1s), although some amino acid changes were found in the mature protein sequence.
33691970	3	44	theme	wheat	382:386	arg1	landraces					388:396	45 Spanish common wheat landraces	364:396	45 Spanish common wheat landraces from Andalusia (southern Spain)	364:428	The waxy protein composition of 45 Spanish common wheat landraces from Andalusia (southern Spain) was evaluated.
33691970	4	45	theme	Wx-A1	496:500	arg1	gene					502:505	the Wx-A1 gene	492:505	the Wx-A1 gene	492:505	Within these materials, five novel alleles for the Wx-A1 gene were detected.
33691970	5	46	theme	acid	619:622	arg1	changes					624:630	some amino acid changes	608:630	some amino acid changes	608:630	Four of them showed functional proteins (Wx-A1p, Wx-A1q, Wx-A1r and Wx-A1s), although some amino acid changes were found in the mature protein sequence.
33691970	3	47	from	composition	349:359	arg1	Spain					423:427	southern Spain	414:427	southern Spain	414:427	The waxy protein composition of 45 Spanish common wheat landraces from Andalusia (southern Spain) was evaluated.
33691970	3	47	from	composition	349:359	arg1	Andalusia					403:411	Andalusia	403:411	Andalusia (southern Spain)	403:428	The waxy protein composition of 45 Spanish common wheat landraces from Andalusia (southern Spain) was evaluated.
33691970	2	48	theme	starch	216:221	arg1	synthases					223:231	several starch synthases	208:231	several starch synthases	208:231	Although several starch synthases are responsible for its synthesis, only the waxy protein is associated with the amylose synthesis.
33691970	6	49	theme	them	691:694	arg1	Wx-A1t					697:702	Wx-A1t	697:702	Wx-A1t	697:702	However, one of them (Wx-A1t) exhibited loss of the Wx-A1 protein, and its base sequence contained one large insert (1,073 bp) in the tenth exon, that interrupted the ORF of the Wx-A1 gene.
33691970	6	49	theme	them	691:694	arg1	them					691:694	them	691:694	them	691:694	However, one of them (Wx-A1t) exhibited loss of the Wx-A1 protein, and its base sequence contained one large insert (1,073 bp) in the tenth exon, that interrupted the ORF of the Wx-A1 gene.
33691970	6	49	theme	them	691:694	arg1	one					684:686	one	684:686	one	684:686	However, one of them (Wx-A1t) exhibited loss of the Wx-A1 protein, and its base sequence contained one large insert (1,073 bp) in the tenth exon, that interrupted the ORF of the Wx-A1 gene.
33691970	9	50	theme	alternative	1300:1310	arg1	crops					1312:1316	alternative crops	1300:1316	alternative crops	1300:1316	In conclusion, the evaluation of old wheat landraces showed that, in addition to their use as alternative crops, these materials could be a useful source of interesting genes in wheat quality improvement.
33691970	3	51	theme	landraces	388:396	arg1	composition					349:359	The waxy protein composition	332:359	The waxy protein composition of 45 Spanish common wheat landraces from Andalusia (southern Spain)	332:428	The waxy protein composition of 45 Spanish common wheat landraces from Andalusia (southern Spain) was evaluated.
33691970	6	52	theme	base	750:753	arg1	sequence					755:762	its base sequence	746:762	its base sequence	746:762	However, one of them (Wx-A1t) exhibited loss of the Wx-A1 protein, and its base sequence contained one large insert (1,073 bp) in the tenth exon, that interrupted the ORF of the Wx-A1 gene.
33691970	2	53	theme	several	208:214	arg1	synthases					223:231	several starch synthases	208:231	several starch synthases	208:231	Although several starch synthases are responsible for its synthesis, only the waxy protein is associated with the amylose synthesis.
33691970	6	54	contain	contained	764:772	arg2	insert					784:789	one large insert	774:789	one large insert (1,073 bp) in the tenth exon, that interrupted the ORF of the Wx-A1 gene	774:862	However, one of them (Wx-A1t) exhibited loss of the Wx-A1 protein, and its base sequence contained one large insert (1,073 bp) in the tenth exon, that interrupted the ORF of the Wx-A1 gene.
33691970	6	54	contain	contained	764:772	arg2	bp					798:799	1,073 bp	792:799	1,073 bp	792:799	However, one of them (Wx-A1t) exhibited loss of the Wx-A1 protein, and its base sequence contained one large insert (1,073 bp) in the tenth exon, that interrupted the ORF of the Wx-A1 gene.
33691970	6	54	contain	contained	764:772	arg1	sequence					755:762	its base sequence	746:762	its base sequence	746:762	However, one of them (Wx-A1t) exhibited loss of the Wx-A1 protein, and its base sequence contained one large insert (1,073 bp) in the tenth exon, that interrupted the ORF of the Wx-A1 gene.
33691970	9	55	dep	use	1293:1295	arg1	addition					1275:1282	addition	1275:1282	addition	1275:1282	In conclusion, the evaluation of old wheat landraces showed that, in addition to their use as alternative crops, these materials could be a useful source of interesting genes in wheat quality improvement.
33691970	5	56	theme	them	530:533	arg1	Four					522:525	Four	522:525	Four	522:525	Four of them showed functional proteins (Wx-A1p, Wx-A1q, Wx-A1r and Wx-A1s), although some amino acid changes were found in the mature protein sequence.
33691970	5	56	theme	them	530:533	arg1	them					530:533	them	530:533	them	530:533	Four of them showed functional proteins (Wx-A1p, Wx-A1q, Wx-A1r and Wx-A1s), although some amino acid changes were found in the mature protein sequence.
33691970	1	57	theme	Wheat	124:128	arg1	starch					130:135	Wheat starch	124:135	Wheat starch	124:135	Wheat starch is composed of two glucose polymers, amylose and amylopectin.
33691970	9	58	theme	interesting	1363:1373	arg1	genes					1375:1379	interesting genes	1363:1379	interesting genes	1363:1379	In conclusion, the evaluation of old wheat landraces showed that, in addition to their use as alternative crops, these materials could be a useful source of interesting genes in wheat quality improvement.
33691970	9	59	theme	useful	1346:1351	arg1	source					1353:1358	a useful source	1344:1358	a useful source of interesting genes in wheat quality improvement	1344:1408	In conclusion, the evaluation of old wheat landraces showed that, in addition to their use as alternative crops, these materials could be a useful source of interesting genes in wheat quality improvement.
33691970	9	59	theme	useful	1346:1351	arg1	materials					1325:1333	these materials	1319:1333	these materials	1319:1333	In conclusion, the evaluation of old wheat landraces showed that, in addition to their use as alternative crops, these materials could be a useful source of interesting genes in wheat quality improvement.
33691970	2	60	theme	amylose	313:319	arg1	synthesis					321:329	the amylose synthesis	309:329	the amylose synthesis	309:329	Although several starch synthases are responsible for its synthesis, only the waxy protein is associated with the amylose synthesis.
33691970	9	61	from	source	1353:1358	arg1	improvement					1398:1408	wheat quality improvement	1384:1408	wheat quality improvement	1384:1408	In conclusion, the evaluation of old wheat landraces showed that, in addition to their use as alternative crops, these materials could be a useful source of interesting genes in wheat quality improvement.
33691970	0	62	theme	common	58:63	arg1	wheat					65:69	common wheat	58:69	common wheat	58:69	Molecular characterization of five novel Wx-A1 alleles in common wheat including one silent allele by transposon insertion.
33691970	6	63	theme	tenth	809:813	arg1	exon					815:818	the tenth exon	805:818	the tenth exon	805:818	However, one of them (Wx-A1t) exhibited loss of the Wx-A1 protein, and its base sequence contained one large insert (1,073 bp) in the tenth exon, that interrupted the ORF of the Wx-A1 gene.
33691970	5	64	dep	proteins	553:560	arg1	Wx-A1s					590:595	Wx-A1s	590:595	Wx-A1s	590:595	Four of them showed functional proteins (Wx-A1p, Wx-A1q, Wx-A1r and Wx-A1s), although some amino acid changes were found in the mature protein sequence.
33691970	5	64	dep	proteins	553:560	arg1	proteins					553:560	functional proteins	542:560	functional proteins (Wx-A1p, Wx-A1q, Wx-A1r and Wx-A1s)	542:596	Four of them showed functional proteins (Wx-A1p, Wx-A1q, Wx-A1r and Wx-A1s), although some amino acid changes were found in the mature protein sequence.
33691970	5	64	dep	proteins	553:560	arg1	Wx-A1r					579:584	Wx-A1r	579:584	Wx-A1r	579:584	Four of them showed functional proteins (Wx-A1p, Wx-A1q, Wx-A1r and Wx-A1s), although some amino acid changes were found in the mature protein sequence.
33691970	5	64	dep	proteins	553:560	arg1	Wx-A1q					571:576	Wx-A1q	571:576	Wx-A1q	571:576	Four of them showed functional proteins (Wx-A1p, Wx-A1q, Wx-A1r and Wx-A1s), although some amino acid changes were found in the mature protein sequence.
33691970	5	64	dep	proteins	553:560	arg1	Wx-A1p					563:568	Wx-A1p	563:568	Wx-A1p	563:568	Four of them showed functional proteins (Wx-A1p, Wx-A1q, Wx-A1r and Wx-A1s), although some amino acid changes were found in the mature protein sequence.
33691970	6	65	from	insert	784:789	arg1	exon					815:818	the tenth exon	805:818	the tenth exon	805:818	However, one of them (Wx-A1t) exhibited loss of the Wx-A1 protein, and its base sequence contained one large insert (1,073 bp) in the tenth exon, that interrupted the ORF of the Wx-A1 gene.
32409054	5	0	theme	drug	1002:1005	arg1	lenses					887:892	three-dimensional chitosan lenses	860:892	three-dimensional chitosan lenses obtained from an insect's (Tabanus bovinus) compound eyes, with the original shape intact,	860:983	In this study, for the first time, three-dimensional chitosan lenses obtained from an insect's (Tabanus bovinus) compound eyes, with the original shape intact, were tested as a drug carrier.
32409054	5	0	theme	drug	1002:1005	arg1	carrier					1007:1013	a drug carrier	1000:1013	a drug carrier	1000:1013	In this study, for the first time, three-dimensional chitosan lenses obtained from an insect's (Tabanus bovinus) compound eyes, with the original shape intact, were tested as a drug carrier.
32409054	3	1	theme	desired	671:677	arg1	shape					679:683	the desired shape	667:683	the desired shape	667:683	Using the produced chitosan gel after dissolving it in weak acids has advantages, such as ease of processing for loading the required amount of active substance and making the desired shape and size.
32409054	10	2	theme	lateral	1556:1562	arg1	assay					1569:1573	lateral flow assay	1556:1573	lateral flow assay	1556:1573	Both chitin and chitosan membranes will be improved for lateral flow assay, and these membranes can be tested for other bioengineering applications in further studies.
32409054	9	3	theme	carrier	1482:1488	arg1	material					1490:1497	a drug carrier material	1475:1497	a drug carrier material	1475:1497	It was observed that chitosan membrane could be used as a drug carrier material.
32409054	9	3	theme	carrier	1482:1488	arg1	membrane					1449:1456	chitosan membrane	1440:1456	chitosan membrane	1440:1456	It was observed that chitosan membrane could be used as a drug carrier material.
32409054	8	4	theme	elemental	1300:1308	arg1	analysis					1310:1317	elemental analysis	1300:1317	elemental analysis	1300:1317	Chitin and chitosan membranes obtained from insect corneal lenses were characterized by using FTIR, TGA, elemental analysis, and surface wettability analysis as well as stereo, binocular, and scanning electron microscopies.
32409054	1	5	theme	non-toxic	212:220	arg1	nature					287:292	its non-toxic, biodegradable, biocompatible, antimicrobial, and anti-oxidative nature	208:292	its non-toxic, biodegradable, biocompatible, antimicrobial, and anti-oxidative nature	208:292	Chitosan is an indispensable biopolymer for use as a drug carrier thanks to its non-toxic, biodegradable, biocompatible, antimicrobial, and anti-oxidative nature.
32409054	3	6	theme	required	620:627	arg1	amount					629:634	the required amount	616:634	the required amount of active substance	616:654	Using the produced chitosan gel after dissolving it in weak acids has advantages, such as ease of processing for loading the required amount of active substance and making the desired shape and size.
32409054	3	6	theme	required	620:627	arg1	substance					646:654	active substance	639:654	active substance	639:654	Using the produced chitosan gel after dissolving it in weak acids has advantages, such as ease of processing for loading the required amount of active substance and making the desired shape and size.
32409054	8	7	theme	Chitin	1195:1200	arg1	membranes					1215:1223	Chitin and chitosan membranes	1195:1223	Chitin and chitosan membranes obtained from insect corneal lenses	1195:1259	Chitin and chitosan membranes obtained from insect corneal lenses were characterized by using FTIR, TGA, elemental analysis, and surface wettability analysis as well as stereo, binocular, and scanning electron microscopies.
32409054	5	8	theme	three-dimensional	860:876	arg1	carrier					1007:1013	a drug carrier	1000:1013	a drug carrier	1000:1013	In this study, for the first time, three-dimensional chitosan lenses obtained from an insect's (Tabanus bovinus) compound eyes, with the original shape intact, were tested as a drug carrier.
32409054	5	8	theme	three-dimensional	860:876	arg1	lenses					887:892	three-dimensional chitosan lenses	860:892	three-dimensional chitosan lenses obtained from an insect's (Tabanus bovinus) compound eyes, with the original shape intact,	860:983	In this study, for the first time, three-dimensional chitosan lenses obtained from an insect's (Tabanus bovinus) compound eyes, with the original shape intact, were tested as a drug carrier.
32409054	8	9	theme	electron	1396:1403	arg1	microscopies					1405:1416	scanning electron microscopies	1387:1416	scanning electron microscopies	1387:1416	Chitin and chitosan membranes obtained from insect corneal lenses were characterized by using FTIR, TGA, elemental analysis, and surface wettability analysis as well as stereo, binocular, and scanning electron microscopies.
32409054	0	10	theme	drug	76:79	arg1	carrier					81:87	a drug carrier	74:87	a drug carrier	74:87	Usage of natural chitosan membrane obtained from insect corneal lenses as a drug carrier and its potential for point of care tests.
32409054	5	11	theme	chitosan	878:885	arg1	carrier					1007:1013	a drug carrier	1000:1013	a drug carrier	1000:1013	In this study, for the first time, three-dimensional chitosan lenses obtained from an insect's (Tabanus bovinus) compound eyes, with the original shape intact, were tested as a drug carrier.
32409054	5	11	theme	chitosan	878:885	arg1	lenses					887:892	three-dimensional chitosan lenses	860:892	three-dimensional chitosan lenses obtained from an insect's (Tabanus bovinus) compound eyes, with the original shape intact,	860:983	In this study, for the first time, three-dimensional chitosan lenses obtained from an insect's (Tabanus bovinus) compound eyes, with the original shape intact, were tested as a drug carrier.
32409054	1	12	theme	biodegradable	223:235	arg1	nature					287:292	its non-toxic, biodegradable, biocompatible, antimicrobial, and anti-oxidative nature	208:292	its non-toxic, biodegradable, biocompatible, antimicrobial, and anti-oxidative nature	208:292	Chitosan is an indispensable biopolymer for use as a drug carrier thanks to its non-toxic, biodegradable, biocompatible, antimicrobial, and anti-oxidative nature.
32409054	9	13	theme	chitosan	1440:1447	arg1	membrane					1449:1456	chitosan membrane	1440:1456	chitosan membrane	1440:1456	It was observed that chitosan membrane could be used as a drug carrier material.
32409054	9	13	theme	chitosan	1440:1447	arg1	material					1490:1497	a drug carrier material	1475:1497	a drug carrier material	1475:1497	It was observed that chitosan membrane could be used as a drug carrier material.
32409054	8	14	theme	chitosan	1206:1213	arg1	membranes					1215:1223	Chitin and chitosan membranes	1195:1223	Chitin and chitosan membranes obtained from insect corneal lenses	1195:1259	Chitin and chitosan membranes obtained from insect corneal lenses were characterized by using FTIR, TGA, elemental analysis, and surface wettability analysis as well as stereo, binocular, and scanning electron microscopies.
32409054	5	15	dep	eyes	947:950	arg1	bovinus					929:935	Tabanus bovinus	921:935	Tabanus bovinus	921:935	In this study, for the first time, three-dimensional chitosan lenses obtained from an insect's (Tabanus bovinus) compound eyes, with the original shape intact, were tested as a drug carrier.
32409054	3	16	theme	active	639:644	arg1	substance					646:654	active substance	639:654	active substance	639:654	Using the produced chitosan gel after dissolving it in weak acids has advantages, such as ease of processing for loading the required amount of active substance and making the desired shape and size.
32409054	10	17	from	applications	1635:1646	arg1	studies					1659:1665	further studies	1651:1665	further studies	1651:1665	Both chitin and chitosan membranes will be improved for lateral flow assay, and these membranes can be tested for other bioengineering applications in further studies.
32409054	2	18	theme	weak	350:353	arg1	acids					355:359	weak acids	350:359	weak acids	350:359	In previous studies, chitosan was first dissolved into weak acids and formed into gel, then used for carrying pharmaceutically active compounds such as nanoparticles, capsules, composites, and films.
32409054	3	19	theme	substance	646:654	arg1	amount					629:634	the required amount	616:634	the required amount of active substance	616:654	Using the produced chitosan gel after dissolving it in weak acids has advantages, such as ease of processing for loading the required amount of active substance and making the desired shape and size.
32409054	3	19	theme	substance	646:654	arg1	substance					646:654	active substance	639:654	active substance	639:654	Using the produced chitosan gel after dissolving it in weak acids has advantages, such as ease of processing for loading the required amount of active substance and making the desired shape and size.
32409054	3	20	theme	chitosan	514:521	arg1	gel					523:525	the produced chitosan gel	501:525	the produced chitosan gel	501:525	Using the produced chitosan gel after dissolving it in weak acids has advantages, such as ease of processing for loading the required amount of active substance and making the desired shape and size.
32409054	4	21	theme	thermal	806:812	arg1	stability					814:822	thermal stability	806:822	thermal stability	806:822	However, dissolved chitosan loses some of its natural properties such as fibrous structure, crystallinity, and thermal stability.
32409054	1	22	theme	indispensable	147:159	arg1	biopolymer					161:170	an indispensable biopolymer	144:170	an indispensable biopolymer for use as a drug carrier thanks to its non-toxic, biodegradable, biocompatible, antimicrobial, and anti-oxidative nature	144:292	Chitosan is an indispensable biopolymer for use as a drug carrier thanks to its non-toxic, biodegradable, biocompatible, antimicrobial, and anti-oxidative nature.
32409054	1	22	theme	indispensable	147:159	arg1	Chitosan					132:139	Chitosan	132:139	Chitosan	132:139	Chitosan is an indispensable biopolymer for use as a drug carrier thanks to its non-toxic, biodegradable, biocompatible, antimicrobial, and anti-oxidative nature.
32409054	6	23	theme	chitosan	1057:1064	arg1	membrane					1066:1073	chitosan membrane	1057:1073	chitosan membrane	1057:1073	A model drug, quercetin, was loaded into chitosan membrane, and its release profile was examined.
32409054	8	24	theme	scanning	1387:1394	arg1	microscopies					1405:1416	scanning electron microscopies	1387:1416	scanning electron microscopies	1387:1416	Chitin and chitosan membranes obtained from insect corneal lenses were characterized by using FTIR, TGA, elemental analysis, and surface wettability analysis as well as stereo, binocular, and scanning electron microscopies.
32409054	4	25	theme	fibrous	768:774	arg1	structure					776:784	fibrous structure	768:784	fibrous structure	768:784	However, dissolved chitosan loses some of its natural properties such as fibrous structure, crystallinity, and thermal stability.
32409054	5	26	theme	first	848:852	arg1	time					854:857	the first time	844:857	the first time	844:857	In this study, for the first time, three-dimensional chitosan lenses obtained from an insect's (Tabanus bovinus) compound eyes, with the original shape intact, were tested as a drug carrier.
32409054	0	27	theme	chitosan	17:24	arg1	membrane					26:33	natural chitosan membrane	9:33	natural chitosan membrane	9:33	Usage of natural chitosan membrane obtained from insect corneal lenses as a drug carrier and its potential for point of care tests.
32409054	1	28	theme	biocompatible	238:250	arg1	nature					287:292	its non-toxic, biodegradable, biocompatible, antimicrobial, and anti-oxidative nature	208:292	its non-toxic, biodegradable, biocompatible, antimicrobial, and anti-oxidative nature	208:292	Chitosan is an indispensable biopolymer for use as a drug carrier thanks to its non-toxic, biodegradable, biocompatible, antimicrobial, and anti-oxidative nature.
32409054	10	29	theme	other	1614:1618	arg1	applications					1635:1646	other bioengineering applications	1614:1646	other bioengineering applications in further studies	1614:1665	Both chitin and chitosan membranes will be improved for lateral flow assay, and these membranes can be tested for other bioengineering applications in further studies.
32409054	6	30	theme	model	1018:1022	arg1	drug					1024:1027	A model drug	1016:1027	A model drug	1016:1027	A model drug, quercetin, was loaded into chitosan membrane, and its release profile was examined.
32409054	6	30	theme	model	1018:1022	arg1	quercetin					1030:1038	quercetin	1030:1038	quercetin	1030:1038	A model drug, quercetin, was loaded into chitosan membrane, and its release profile was examined.
32409054	10	31	dep	chitin	1505:1510	arg1	membranes					1525:1533	membranes	1525:1533	membranes	1525:1533	Both chitin and chitosan membranes will be improved for lateral flow assay, and these membranes can be tested for other bioengineering applications in further studies.
32409054	0	32	theme	natural	9:15	arg1	membrane					26:33	natural chitosan membrane	9:33	natural chitosan membrane	9:33	Usage of natural chitosan membrane obtained from insect corneal lenses as a drug carrier and its potential for point of care tests.
32409054	8	33	theme	surface	1324:1330	arg1	analysis					1344:1351	surface wettability analysis	1324:1351	surface wettability analysis	1324:1351	Chitin and chitosan membranes obtained from insect corneal lenses were characterized by using FTIR, TGA, elemental analysis, and surface wettability analysis as well as stereo, binocular, and scanning electron microscopies.
32409054	3	34	theme	produced	505:512	arg1	gel					523:525	the produced chitosan gel	501:525	the produced chitosan gel	501:525	Using the produced chitosan gel after dissolving it in weak acids has advantages, such as ease of processing for loading the required amount of active substance and making the desired shape and size.
32409054	1	35	theme	antimicrobial	253:265	arg1	nature					287:292	its non-toxic, biodegradable, biocompatible, antimicrobial, and anti-oxidative nature	208:292	its non-toxic, biodegradable, biocompatible, antimicrobial, and anti-oxidative nature	208:292	Chitosan is an indispensable biopolymer for use as a drug carrier thanks to its non-toxic, biodegradable, biocompatible, antimicrobial, and anti-oxidative nature.
32409054	5	36	theme	original	962:969	arg1	shape					971:975	the original shape intact	958:982	the original shape intact	958:982	In this study, for the first time, three-dimensional chitosan lenses obtained from an insect's (Tabanus bovinus) compound eyes, with the original shape intact, were tested as a drug carrier.
32409054	7	37	dep	chitin	1164:1169	arg1	membranes					1184:1192	membranes	1184:1192	membranes	1184:1192	Also, a point-of-care test was conducted for both chitin and chitosan membranes.
32409054	9	38	theme	drug	1477:1480	arg1	material					1490:1497	a drug carrier material	1475:1497	a drug carrier material	1475:1497	It was observed that chitosan membrane could be used as a drug carrier material.
32409054	9	38	theme	drug	1477:1480	arg1	membrane					1449:1456	chitosan membrane	1440:1456	chitosan membrane	1440:1456	It was observed that chitosan membrane could be used as a drug carrier material.
32409054	2	39	theme	active	422:427	arg1	nanoparticles					447:459	nanoparticles	447:459	nanoparticles	447:459	In previous studies, chitosan was first dissolved into weak acids and formed into gel, then used for carrying pharmaceutically active compounds such as nanoparticles, capsules, composites, and films.
32409054	2	39	theme	active	422:427	arg1	composites					472:481	composites	472:481	composites	472:481	In previous studies, chitosan was first dissolved into weak acids and formed into gel, then used for carrying pharmaceutically active compounds such as nanoparticles, capsules, composites, and films.
32409054	2	39	theme	active	422:427	arg1	films					488:492	films	488:492	films	488:492	In previous studies, chitosan was first dissolved into weak acids and formed into gel, then used for carrying pharmaceutically active compounds such as nanoparticles, capsules, composites, and films.
32409054	2	39	theme	active	422:427	arg1	capsules					462:469	capsules	462:469	capsules	462:469	In previous studies, chitosan was first dissolved into weak acids and formed into gel, then used for carrying pharmaceutically active compounds such as nanoparticles, capsules, composites, and films.
32409054	2	39	theme	active	422:427	arg1	compounds					429:437	pharmaceutically active compounds	405:437	pharmaceutically active compounds such as nanoparticles, capsules, composites, and films	405:492	In previous studies, chitosan was first dissolved into weak acids and formed into gel, then used for carrying pharmaceutically active compounds such as nanoparticles, capsules, composites, and films.
32409054	0	40	theme	membrane	26:33	arg1	Usage					0:4	Usage	0:4	Usage of natural chitosan membrane	0:33	Usage of natural chitosan membrane obtained from insect corneal lenses as a drug carrier and its potential for point of care tests.
32409054	6	41	theme	release	1084:1090	arg1	profile					1092:1098	its release profile	1080:1098	its release profile	1080:1098	A model drug, quercetin, was loaded into chitosan membrane, and its release profile was examined.
32409054	5	42	with	eyes	947:950	arg1	shape					971:975	the original shape intact	958:982	the original shape intact	958:982	In this study, for the first time, three-dimensional chitosan lenses obtained from an insect's (Tabanus bovinus) compound eyes, with the original shape intact, were tested as a drug carrier.
32409054	0	43	theme	insect	49:54	arg1	lenses					64:69	insect corneal lenses	49:69	insect corneal lenses	49:69	Usage of natural chitosan membrane obtained from insect corneal lenses as a drug carrier and its potential for point of care tests.
32409054	0	44	theme	tests	125:129	arg1	point					111:115	point	111:115	point of care tests	111:129	Usage of natural chitosan membrane obtained from insect corneal lenses as a drug carrier and its potential for point of care tests.
32409054	5	45	theme	intact	977:982	arg1	shape					971:975	the original shape intact	958:982	the original shape intact	958:982	In this study, for the first time, three-dimensional chitosan lenses obtained from an insect's (Tabanus bovinus) compound eyes, with the original shape intact, were tested as a drug carrier.
32409054	10	46	theme	bioengineering	1620:1633	arg1	applications					1635:1646	other bioengineering applications	1614:1646	other bioengineering applications in further studies	1614:1665	Both chitin and chitosan membranes will be improved for lateral flow assay, and these membranes can be tested for other bioengineering applications in further studies.
32409054	7	47	theme	point-of-care	1122:1134	arg1	test					1136:1139	a point-of-care test	1120:1139	a point-of-care test	1120:1139	Also, a point-of-care test was conducted for both chitin and chitosan membranes.
32409054	1	48	theme	anti-oxidative	272:285	arg1	nature					287:292	its non-toxic, biodegradable, biocompatible, antimicrobial, and anti-oxidative nature	208:292	its non-toxic, biodegradable, biocompatible, antimicrobial, and anti-oxidative nature	208:292	Chitosan is an indispensable biopolymer for use as a drug carrier thanks to its non-toxic, biodegradable, biocompatible, antimicrobial, and anti-oxidative nature.
32409054	0	49	theme	care	120:123	arg1	tests					125:129	care tests	120:129	care tests	120:129	Usage of natural chitosan membrane obtained from insect corneal lenses as a drug carrier and its potential for point of care tests.
32409054	5	50	theme	compound	938:945	arg1	eyes					947:950	an insect's (Tabanus bovinus) compound eyes	908:950	an insect's (Tabanus bovinus) compound eyes	908:950	In this study, for the first time, three-dimensional chitosan lenses obtained from an insect's (Tabanus bovinus) compound eyes, with the original shape intact, were tested as a drug carrier.
32409054	4	51	theme	natural	741:747	arg1	crystallinity					787:799	crystallinity	787:799	crystallinity	787:799	However, dissolved chitosan loses some of its natural properties such as fibrous structure, crystallinity, and thermal stability.
32409054	4	51	theme	natural	741:747	arg1	structure					776:784	fibrous structure	768:784	fibrous structure	768:784	However, dissolved chitosan loses some of its natural properties such as fibrous structure, crystallinity, and thermal stability.
32409054	4	51	theme	natural	741:747	arg1	stability					814:822	thermal stability	806:822	thermal stability	806:822	However, dissolved chitosan loses some of its natural properties such as fibrous structure, crystallinity, and thermal stability.
32409054	4	51	theme	natural	741:747	arg1	properties					749:758	its natural properties	737:758	its natural properties such as fibrous structure, crystallinity, and thermal stability	737:822	However, dissolved chitosan loses some of its natural properties such as fibrous structure, crystallinity, and thermal stability.
32409054	9	52	used	used	1467:1470	arg2	material					1490:1497	a drug carrier material	1475:1497	a drug carrier material	1475:1497	It was observed that chitosan membrane could be used as a drug carrier material.
32409054	9	52	used	used	1467:1470	arg2	membrane					1449:1456	chitosan membrane	1440:1456	chitosan membrane	1440:1456	It was observed that chitosan membrane could be used as a drug carrier material.
32409054	2	53	theme	previous	298:305	arg1	studies					307:313	previous studies	298:313	previous studies	298:313	In previous studies, chitosan was first dissolved into weak acids and formed into gel, then used for carrying pharmaceutically active compounds such as nanoparticles, capsules, composites, and films.
32409054	8	54	theme	corneal	1246:1252	arg1	lenses					1254:1259	insect corneal lenses	1239:1259	insect corneal lenses	1239:1259	Chitin and chitosan membranes obtained from insect corneal lenses were characterized by using FTIR, TGA, elemental analysis, and surface wettability analysis as well as stereo, binocular, and scanning electron microscopies.
32409054	8	55	theme	insect	1239:1244	arg1	lenses					1254:1259	insect corneal lenses	1239:1259	insect corneal lenses	1239:1259	Chitin and chitosan membranes obtained from insect corneal lenses were characterized by using FTIR, TGA, elemental analysis, and surface wettability analysis as well as stereo, binocular, and scanning electron microscopies.
32409054	1	56	theme	drug	185:188	arg1	carrier					190:196	a drug carrier	183:196	a drug carrier thanks to its non-toxic, biodegradable, biocompatible, antimicrobial, and anti-oxidative nature	183:292	Chitosan is an indispensable biopolymer for use as a drug carrier thanks to its non-toxic, biodegradable, biocompatible, antimicrobial, and anti-oxidative nature.
32409054	8	57	theme	wettability	1332:1342	arg1	analysis					1344:1351	surface wettability analysis	1324:1351	surface wettability analysis	1324:1351	Chitin and chitosan membranes obtained from insect corneal lenses were characterized by using FTIR, TGA, elemental analysis, and surface wettability analysis as well as stereo, binocular, and scanning electron microscopies.
32409054	4	58	theme	dissolved	704:712	arg1	chitosan					714:721	dissolved chitosan	704:721	dissolved chitosan	704:721	However, dissolved chitosan loses some of its natural properties such as fibrous structure, crystallinity, and thermal stability.
32409054	3	59	theme	processing	593:602	arg1	ease					585:588	ease	585:588	ease of processing for loading the required amount of active substance and making the desired shape and size	585:692	Using the produced chitosan gel after dissolving it in weak acids has advantages, such as ease of processing for loading the required amount of active substance and making the desired shape and size.
32409054	3	60	theme	weak	550:553	arg1	acids					555:559	weak acids	550:559	weak acids	550:559	Using the produced chitosan gel after dissolving it in weak acids has advantages, such as ease of processing for loading the required amount of active substance and making the desired shape and size.
32409054	1	61	theme	thanks	198:203	arg1	carrier					190:196	a drug carrier	183:196	a drug carrier thanks to its non-toxic, biodegradable, biocompatible, antimicrobial, and anti-oxidative nature	183:292	Chitosan is an indispensable biopolymer for use as a drug carrier thanks to its non-toxic, biodegradable, biocompatible, antimicrobial, and anti-oxidative nature.
32409054	0	62	theme	corneal	56:62	arg1	lenses					64:69	insect corneal lenses	49:69	insect corneal lenses	49:69	Usage of natural chitosan membrane obtained from insect corneal lenses as a drug carrier and its potential for point of care tests.
32409054	10	63	theme	further	1651:1657	arg1	studies					1659:1665	further studies	1651:1665	further studies	1651:1665	Both chitin and chitosan membranes will be improved for lateral flow assay, and these membranes can be tested for other bioengineering applications in further studies.
32409054	10	64	theme	flow	1564:1567	arg1	assay					1569:1573	lateral flow assay	1556:1573	lateral flow assay	1556:1573	Both chitin and chitosan membranes will be improved for lateral flow assay, and these membranes can be tested for other bioengineering applications in further studies.
31981704	3	0	theme	dried	762:766	arg1	agglomerate					768:778	the final dried agglomerate	752:778	the final dried agglomerate	752:778	The percentage soluble fraction of the components in the inlet feed had the largest influence on the morphology of the final dried agglomerate.
31981704	4	1	theme	components	941:950	arg1	agglomerates					912:923	agglomerates	912:923	agglomerates of highly mixed components	912:950	Low excipient soluble fraction resulted in an increase in paracetamol surface coating and high excipient soluble fraction produced agglomerates of highly mixed components.
31981704	0	2	theme	agglomerates	79:90	arg1	manufacturing					47:59	manufacturing	47:59	manufacturing of drug/excipient agglomerates	47:90	Crystallo-co-spray drying as a new approach to manufacturing of drug/excipient agglomerates: Impact of processing on the properties of paracetamol and lactose mixtures.
31981704	3	3	theme	largest	713:719	arg1	influence					721:729	the largest influence	709:729	the largest influence	709:729	The percentage soluble fraction of the components in the inlet feed had the largest influence on the morphology of the final dried agglomerate.
31981704	2	4	theme	monohydrate	562:572	arg1	groups					601:606	the amide and hydroxyl groups	578:606	groups	601:606	A crystalline agglomerate was obtained with no change to the physical structure of either component throughout both investigations and with possible interactions between the hydroxyl groups of the α-lactose monohydrate and the amide and hydroxyl groups of the paracetamol detected.
31981704	2	4	theme	monohydrate	562:572	arg1	paracetamol					615:625	the paracetamol	611:625	the paracetamol detected	611:634	A crystalline agglomerate was obtained with no change to the physical structure of either component throughout both investigations and with possible interactions between the hydroxyl groups of the α-lactose monohydrate and the amide and hydroxyl groups of the paracetamol detected.
31981704	2	4	theme	monohydrate	562:572	arg1	groups					538:543	the hydroxyl groups	525:543	the hydroxyl groups of the α-lactose monohydrate	525:572	A crystalline agglomerate was obtained with no change to the physical structure of either component throughout both investigations and with possible interactions between the hydroxyl groups of the α-lactose monohydrate and the amide and hydroxyl groups of the paracetamol detected.
31981704	2	4	theme	monohydrate	562:572	arg1	monohydrate					562:572	the α-lactose monohydrate	548:572	the α-lactose monohydrate	548:572	A crystalline agglomerate was obtained with no change to the physical structure of either component throughout both investigations and with possible interactions between the hydroxyl groups of the α-lactose monohydrate and the amide and hydroxyl groups of the paracetamol detected.
31981704	4	5	theme	soluble	795:801	arg1	fraction					803:810	Low excipient soluble fraction	781:810	Low excipient soluble fraction	781:810	Low excipient soluble fraction resulted in an increase in paracetamol surface coating and high excipient soluble fraction produced agglomerates of highly mixed components.
31981704	3	6	from	feed	700:703	arg1	fraction					660:667	The percentage soluble fraction	637:667	The percentage soluble fraction of the components in the inlet feed	637:703	The percentage soluble fraction of the components in the inlet feed had the largest influence on the morphology of the final dried agglomerate.
31981704	2	7	theme	α-lactose	552:560	arg1	monohydrate					562:572	the α-lactose monohydrate	548:572	the α-lactose monohydrate	548:572	A crystalline agglomerate was obtained with no change to the physical structure of either component throughout both investigations and with possible interactions between the hydroxyl groups of the α-lactose monohydrate and the amide and hydroxyl groups of the paracetamol detected.
31981704	2	8	theme	paracetamol	615:625	arg1	groups					601:606	the amide and hydroxyl groups	578:606	groups	601:606	A crystalline agglomerate was obtained with no change to the physical structure of either component throughout both investigations and with possible interactions between the hydroxyl groups of the α-lactose monohydrate and the amide and hydroxyl groups of the paracetamol detected.
31981704	2	8	theme	paracetamol	615:625	arg1	paracetamol					615:625	the paracetamol	611:625	the paracetamol detected	611:634	A crystalline agglomerate was obtained with no change to the physical structure of either component throughout both investigations and with possible interactions between the hydroxyl groups of the α-lactose monohydrate and the amide and hydroxyl groups of the paracetamol detected.
31981704	2	8	theme	paracetamol	615:625	arg1	groups					538:543	the hydroxyl groups	525:543	the hydroxyl groups of the α-lactose monohydrate	525:572	A crystalline agglomerate was obtained with no change to the physical structure of either component throughout both investigations and with possible interactions between the hydroxyl groups of the α-lactose monohydrate and the amide and hydroxyl groups of the paracetamol detected.
31981704	2	8	theme	paracetamol	615:625	arg1	monohydrate					562:572	the α-lactose monohydrate	548:572	the α-lactose monohydrate	548:572	A crystalline agglomerate was obtained with no change to the physical structure of either component throughout both investigations and with possible interactions between the hydroxyl groups of the α-lactose monohydrate and the amide and hydroxyl groups of the paracetamol detected.
31981704	4	9	theme	excipient	785:793	arg1	fraction					803:810	Low excipient soluble fraction	781:810	Low excipient soluble fraction	781:810	Low excipient soluble fraction resulted in an increase in paracetamol surface coating and high excipient soluble fraction produced agglomerates of highly mixed components.
31981704	3	10	theme	final	756:760	arg1	agglomerate					768:778	the final dried agglomerate	752:778	the final dried agglomerate	752:778	The percentage soluble fraction of the components in the inlet feed had the largest influence on the morphology of the final dried agglomerate.
31981704	4	11	theme	excipient	876:884	arg1	fraction					894:901	high excipient soluble fraction	871:901	high excipient soluble fraction	871:901	Low excipient soluble fraction resulted in an increase in paracetamol surface coating and high excipient soluble fraction produced agglomerates of highly mixed components.
31981704	0	12	theme	processing	103:112	arg1	Impact					93:98	Impact	93:98	Crystallo-co-spray drying as a new approach to manufacturing of drug/excipient agglomerates: Impact of processing on the properties of paracetamol and lactose mixtures.	0:167	Crystallo-co-spray drying as a new approach to manufacturing of drug/excipient agglomerates: Impact of processing on the properties of paracetamol and lactose mixtures.
31981704	5	13	theme	process	1172:1178	arg1	drying					1219:1224	drying	1219:1224	drying	1219:1224	The use of crystallo-co-spray drying can serve as a method of producing an agglomerated blend of crystalline co-spray dried components leading to possible opportunities for process intensification with the reduction of process steps such as blending, agglomeration, drying and milling into one single stage.
31981704	5	13	theme	process	1172:1178	arg1	agglomeration					1204:1216	agglomeration	1204:1216	agglomeration	1204:1216	The use of crystallo-co-spray drying can serve as a method of producing an agglomerated blend of crystalline co-spray dried components leading to possible opportunities for process intensification with the reduction of process steps such as blending, agglomeration, drying and milling into one single stage.
31981704	5	13	theme	process	1172:1178	arg1	steps					1180:1184	process steps	1172:1184	process steps such as blending, agglomeration, drying and milling into one single stage	1172:1258	The use of crystallo-co-spray drying can serve as a method of producing an agglomerated blend of crystalline co-spray dried components leading to possible opportunities for process intensification with the reduction of process steps such as blending, agglomeration, drying and milling into one single stage.
31981704	5	13	theme	process	1172:1178	arg1	blending					1194:1201	blending	1194:1201	blending	1194:1201	The use of crystallo-co-spray drying can serve as a method of producing an agglomerated blend of crystalline co-spray dried components leading to possible opportunities for process intensification with the reduction of process steps such as blending, agglomeration, drying and milling into one single stage.
31981704	5	13	theme	process	1172:1178	arg1	milling					1230:1236	milling	1230:1236	milling	1230:1236	The use of crystallo-co-spray drying can serve as a method of producing an agglomerated blend of crystalline co-spray dried components leading to possible opportunities for process intensification with the reduction of process steps such as blending, agglomeration, drying and milling into one single stage.
31981704	4	14	theme	high	871:874	arg1	fraction					894:901	high excipient soluble fraction	871:901	high excipient soluble fraction	871:901	Low excipient soluble fraction resulted in an increase in paracetamol surface coating and high excipient soluble fraction produced agglomerates of highly mixed components.
31981704	3	15	from	fraction	660:667	arg1	feed					700:703	the inlet feed	690:703	the inlet feed	690:703	The percentage soluble fraction of the components in the inlet feed had the largest influence on the morphology of the final dried agglomerate.
31981704	1	16	theme	novel	173:177	arg1	process					179:185	The novel process	169:185	The novel process of crystallo-co-spray drying of paracetamol and α-lactose monohydrate	169:255	The novel process of crystallo-co-spray drying of paracetamol and α-lactose monohydrate was investigated by varying the spray dryer inlet temperature and inlet feed solvent composition.
31981704	5	17	theme	steps	1180:1184	arg1	reduction					1159:1167	the reduction	1155:1167	the reduction of process steps such as blending, agglomeration, drying and milling into one single stage	1155:1258	The use of crystallo-co-spray drying can serve as a method of producing an agglomerated blend of crystalline co-spray dried components leading to possible opportunities for process intensification with the reduction of process steps such as blending, agglomeration, drying and milling into one single stage.
31981704	0	18	theme	Crystallo-co-spray	0:17	arg1	drying					19:24	Crystallo-co-spray drying	0:24	Crystallo-co-spray drying as a new approach to manufacturing of drug/excipient agglomerates: Impact of processing on the properties of paracetamol and lactose mixtures.	0:167	Crystallo-co-spray drying as a new approach to manufacturing of drug/excipient agglomerates: Impact of processing on the properties of paracetamol and lactose mixtures.
31981704	1	19	theme	spray	289:293	arg1	temperature					307:317	the spray dryer inlet temperature	285:317	the spray dryer inlet temperature	285:317	The novel process of crystallo-co-spray drying of paracetamol and α-lactose monohydrate was investigated by varying the spray dryer inlet temperature and inlet feed solvent composition.
31981704	4	20	theme	soluble	886:892	arg1	fraction					894:901	high excipient soluble fraction	871:901	high excipient soluble fraction	871:901	Low excipient soluble fraction resulted in an increase in paracetamol surface coating and high excipient soluble fraction produced agglomerates of highly mixed components.
31981704	3	21	contain	had	705:707	arg1	fraction					660:667	The percentage soluble fraction	637:667	The percentage soluble fraction of the components in the inlet feed	637:703	The percentage soluble fraction of the components in the inlet feed had the largest influence on the morphology of the final dried agglomerate.
31981704	3	21	contain	had	705:707	arg2	influence					721:729	the largest influence	709:729	the largest influence	709:729	The percentage soluble fraction of the components in the inlet feed had the largest influence on the morphology of the final dried agglomerate.
31981704	1	22	theme	dryer	295:299	arg1	temperature					307:317	the spray dryer inlet temperature	285:317	the spray dryer inlet temperature	285:317	The novel process of crystallo-co-spray drying of paracetamol and α-lactose monohydrate was investigated by varying the spray dryer inlet temperature and inlet feed solvent composition.
31981704	4	23	theme	Low	781:783	arg1	fraction					803:810	Low excipient soluble fraction	781:810	Low excipient soluble fraction	781:810	Low excipient soluble fraction resulted in an increase in paracetamol surface coating and high excipient soluble fraction produced agglomerates of highly mixed components.
31981704	5	24	theme	co-spray	1062:1069	arg1	components					1077:1086	crystalline co-spray dried components	1050:1086	crystalline co-spray dried components leading to possible opportunities for process intensification with the reduction of process steps such as blending, agglomeration, drying and milling into one single stage	1050:1258	The use of crystallo-co-spray drying can serve as a method of producing an agglomerated blend of crystalline co-spray dried components leading to possible opportunities for process intensification with the reduction of process steps such as blending, agglomeration, drying and milling into one single stage.
31981704	1	25	theme	crystallo-co-spray	190:207	arg1	drying					209:214	crystallo-co-spray drying	190:214	crystallo-co-spray drying of paracetamol and α-lactose monohydrate	190:255	The novel process of crystallo-co-spray drying of paracetamol and α-lactose monohydrate was investigated by varying the spray dryer inlet temperature and inlet feed solvent composition.
31981704	2	26	theme	component	445:453	arg1	structure					425:433	the physical structure	412:433	the physical structure of either component	412:453	A crystalline agglomerate was obtained with no change to the physical structure of either component throughout both investigations and with possible interactions between the hydroxyl groups of the α-lactose monohydrate and the amide and hydroxyl groups of the paracetamol detected.
31981704	1	27	theme	inlet	301:305	arg1	temperature					307:317	the spray dryer inlet temperature	285:317	the spray dryer inlet temperature	285:317	The novel process of crystallo-co-spray drying of paracetamol and α-lactose monohydrate was investigated by varying the spray dryer inlet temperature and inlet feed solvent composition.
31981704	2	28	theme	amide	582:586	arg1	groups					601:606	the amide and hydroxyl groups	578:606	groups	601:606	A crystalline agglomerate was obtained with no change to the physical structure of either component throughout both investigations and with possible interactions between the hydroxyl groups of the α-lactose monohydrate and the amide and hydroxyl groups of the paracetamol detected.
31981704	2	28	theme	amide	582:586	arg1	paracetamol					615:625	the paracetamol	611:625	the paracetamol detected	611:634	A crystalline agglomerate was obtained with no change to the physical structure of either component throughout both investigations and with possible interactions between the hydroxyl groups of the α-lactose monohydrate and the amide and hydroxyl groups of the paracetamol detected.
31981704	2	28	theme	amide	582:586	arg1	monohydrate					562:572	the α-lactose monohydrate	548:572	the α-lactose monohydrate	548:572	A crystalline agglomerate was obtained with no change to the physical structure of either component throughout both investigations and with possible interactions between the hydroxyl groups of the α-lactose monohydrate and the amide and hydroxyl groups of the paracetamol detected.
31981704	5	29	theme	process	1126:1132	arg1	intensification					1134:1148	process intensification	1126:1148	process intensification with the reduction of process steps such as blending, agglomeration, drying and milling into one single stage	1126:1258	The use of crystallo-co-spray drying can serve as a method of producing an agglomerated blend of crystalline co-spray dried components leading to possible opportunities for process intensification with the reduction of process steps such as blending, agglomeration, drying and milling into one single stage.
31981704	1	30	theme	drying	209:214	arg1	process					179:185	The novel process	169:185	The novel process of crystallo-co-spray drying of paracetamol and α-lactose monohydrate	169:255	The novel process of crystallo-co-spray drying of paracetamol and α-lactose monohydrate was investigated by varying the spray dryer inlet temperature and inlet feed solvent composition.
31981704	5	31	theme	single	1247:1252	arg1	stage					1254:1258	one single stage	1243:1258	one single stage	1243:1258	The use of crystallo-co-spray drying can serve as a method of producing an agglomerated blend of crystalline co-spray dried components leading to possible opportunities for process intensification with the reduction of process steps such as blending, agglomeration, drying and milling into one single stage.
31981704	3	32	theme	agglomerate	768:778	arg1	morphology					738:747	the morphology	734:747	the morphology of the final dried agglomerate	734:778	The percentage soluble fraction of the components in the inlet feed had the largest influence on the morphology of the final dried agglomerate.
31981704	0	33	theme	paracetamol	135:145	arg1	properties					121:130	the properties	117:130	the properties of paracetamol and lactose mixtures	117:166	Crystallo-co-spray drying as a new approach to manufacturing of drug/excipient agglomerates: Impact of processing on the properties of paracetamol and lactose mixtures.
31981704	4	34	theme	surface	851:857	arg1	coating					859:865	paracetamol surface coating	839:865	paracetamol surface coating	839:865	Low excipient soluble fraction resulted in an increase in paracetamol surface coating and high excipient soluble fraction produced agglomerates of highly mixed components.
31981704	5	35	with	intensification	1134:1148	arg1	reduction					1159:1167	the reduction	1155:1167	the reduction of process steps such as blending, agglomeration, drying and milling into one single stage	1155:1258	The use of crystallo-co-spray drying can serve as a method of producing an agglomerated blend of crystalline co-spray dried components leading to possible opportunities for process intensification with the reduction of process steps such as blending, agglomeration, drying and milling into one single stage.
31981704	5	36	theme	crystalline	1050:1060	arg1	components					1077:1086	crystalline co-spray dried components	1050:1086	crystalline co-spray dried components leading to possible opportunities for process intensification with the reduction of process steps such as blending, agglomeration, drying and milling into one single stage	1050:1258	The use of crystallo-co-spray drying can serve as a method of producing an agglomerated blend of crystalline co-spray dried components leading to possible opportunities for process intensification with the reduction of process steps such as blending, agglomeration, drying and milling into one single stage.
31981704	0	37	theme	new	31:33	arg1	approach					35:42	a new approach	29:42	a new approach to manufacturing of drug/excipient agglomerates	29:90	Crystallo-co-spray drying as a new approach to manufacturing of drug/excipient agglomerates: Impact of processing on the properties of paracetamol and lactose mixtures.
31981704	2	38	theme	possible	495:502	arg1	interactions					504:515	possible interactions	495:515	possible interactions between the hydroxyl groups of the α-lactose monohydrate and the amide and hydroxyl groups of the paracetamol detected	495:634	A crystalline agglomerate was obtained with no change to the physical structure of either component throughout both investigations and with possible interactions between the hydroxyl groups of the α-lactose monohydrate and the amide and hydroxyl groups of the paracetamol detected.
31981704	4	39	theme	paracetamol	839:849	arg1	coating					859:865	paracetamol surface coating	839:865	paracetamol surface coating	839:865	Low excipient soluble fraction resulted in an increase in paracetamol surface coating and high excipient soluble fraction produced agglomerates of highly mixed components.
31981704	2	40	theme	physical	416:423	arg1	structure					425:433	the physical structure	412:433	the physical structure of either component	412:453	A crystalline agglomerate was obtained with no change to the physical structure of either component throughout both investigations and with possible interactions between the hydroxyl groups of the α-lactose monohydrate and the amide and hydroxyl groups of the paracetamol detected.
31981704	1	41	theme	paracetamol	219:229	arg1	drying					209:214	crystallo-co-spray drying	190:214	crystallo-co-spray drying of paracetamol and α-lactose monohydrate	190:255	The novel process of crystallo-co-spray drying of paracetamol and α-lactose monohydrate was investigated by varying the spray dryer inlet temperature and inlet feed solvent composition.
31981704	1	42	theme	inlet	323:327	arg1	composition					342:352	inlet feed solvent composition	323:352	inlet feed solvent composition	323:352	The novel process of crystallo-co-spray drying of paracetamol and α-lactose monohydrate was investigated by varying the spray dryer inlet temperature and inlet feed solvent composition.
31981704	0	43	theme	lactose	151:157	arg1	properties					121:130	the properties	117:130	the properties of paracetamol and lactose mixtures	117:166	Crystallo-co-spray drying as a new approach to manufacturing of drug/excipient agglomerates: Impact of processing on the properties of paracetamol and lactose mixtures.
31981704	3	44	theme	components	676:685	arg1	fraction					660:667	The percentage soluble fraction	637:667	The percentage soluble fraction of the components in the inlet feed	637:703	The percentage soluble fraction of the components in the inlet feed had the largest influence on the morphology of the final dried agglomerate.
31981704	5	45	theme	components	1077:1086	arg1	blend					1041:1045	an agglomerated blend	1025:1045	an agglomerated blend of crystalline co-spray dried components leading to possible opportunities for process intensification with the reduction of process steps such as blending, agglomeration, drying and milling into one single stage	1025:1258	The use of crystallo-co-spray drying can serve as a method of producing an agglomerated blend of crystalline co-spray dried components leading to possible opportunities for process intensification with the reduction of process steps such as blending, agglomeration, drying and milling into one single stage.
31981704	2	46	theme	crystalline	357:367	arg1	agglomerate					369:379	A crystalline agglomerate	355:379	A crystalline agglomerate	355:379	A crystalline agglomerate was obtained with no change to the physical structure of either component throughout both investigations and with possible interactions between the hydroxyl groups of the α-lactose monohydrate and the amide and hydroxyl groups of the paracetamol detected.
31981704	1	47	theme	feed	329:332	arg1	composition					342:352	inlet feed solvent composition	323:352	inlet feed solvent composition	323:352	The novel process of crystallo-co-spray drying of paracetamol and α-lactose monohydrate was investigated by varying the spray dryer inlet temperature and inlet feed solvent composition.
31981704	4	48	theme	mixed	935:939	arg1	components					941:950	highly mixed components	928:950	highly mixed components	928:950	Low excipient soluble fraction resulted in an increase in paracetamol surface coating and high excipient soluble fraction produced agglomerates of highly mixed components.
31981704	1	49	theme	solvent	334:340	arg1	composition					342:352	inlet feed solvent composition	323:352	inlet feed solvent composition	323:352	The novel process of crystallo-co-spray drying of paracetamol and α-lactose monohydrate was investigated by varying the spray dryer inlet temperature and inlet feed solvent composition.
31981704	5	50	theme	possible	1099:1106	arg1	opportunities					1108:1120	possible opportunities	1099:1120	possible opportunities for process intensification with the reduction of process steps such as blending, agglomeration, drying and milling into one single stage	1099:1258	The use of crystallo-co-spray drying can serve as a method of producing an agglomerated blend of crystalline co-spray dried components leading to possible opportunities for process intensification with the reduction of process steps such as blending, agglomeration, drying and milling into one single stage.
31981704	5	51	theme	agglomerated	1028:1039	arg1	blend					1041:1045	an agglomerated blend	1025:1045	an agglomerated blend of crystalline co-spray dried components leading to possible opportunities for process intensification with the reduction of process steps such as blending, agglomeration, drying and milling into one single stage	1025:1258	The use of crystallo-co-spray drying can serve as a method of producing an agglomerated blend of crystalline co-spray dried components leading to possible opportunities for process intensification with the reduction of process steps such as blending, agglomeration, drying and milling into one single stage.
31981704	5	52	theme	dried	1071:1075	arg1	components					1077:1086	crystalline co-spray dried components	1050:1086	crystalline co-spray dried components leading to possible opportunities for process intensification with the reduction of process steps such as blending, agglomeration, drying and milling into one single stage	1050:1258	The use of crystallo-co-spray drying can serve as a method of producing an agglomerated blend of crystalline co-spray dried components leading to possible opportunities for process intensification with the reduction of process steps such as blending, agglomeration, drying and milling into one single stage.
31981704	5	53	theme	crystallo-co-spray	964:981	arg1	drying					983:988	crystallo-co-spray drying	964:988	crystallo-co-spray drying	964:988	The use of crystallo-co-spray drying can serve as a method of producing an agglomerated blend of crystalline co-spray dried components leading to possible opportunities for process intensification with the reduction of process steps such as blending, agglomeration, drying and milling into one single stage.
31981704	3	54	theme	percentage	641:650	arg1	fraction					660:667	The percentage soluble fraction	637:667	The percentage soluble fraction of the components in the inlet feed	637:703	The percentage soluble fraction of the components in the inlet feed had the largest influence on the morphology of the final dried agglomerate.
31981704	2	55	theme	hydroxyl	592:599	arg1	groups					601:606	the amide and hydroxyl groups	578:606	groups	601:606	A crystalline agglomerate was obtained with no change to the physical structure of either component throughout both investigations and with possible interactions between the hydroxyl groups of the α-lactose monohydrate and the amide and hydroxyl groups of the paracetamol detected.
31981704	2	55	theme	hydroxyl	592:599	arg1	paracetamol					615:625	the paracetamol	611:625	the paracetamol detected	611:634	A crystalline agglomerate was obtained with no change to the physical structure of either component throughout both investigations and with possible interactions between the hydroxyl groups of the α-lactose monohydrate and the amide and hydroxyl groups of the paracetamol detected.
31981704	2	55	theme	hydroxyl	592:599	arg1	monohydrate					562:572	the α-lactose monohydrate	548:572	the α-lactose monohydrate	548:572	A crystalline agglomerate was obtained with no change to the physical structure of either component throughout both investigations and with possible interactions between the hydroxyl groups of the α-lactose monohydrate and the amide and hydroxyl groups of the paracetamol detected.
31981704	3	56	theme	inlet	694:698	arg1	feed					700:703	the inlet feed	690:703	the inlet feed	690:703	The percentage soluble fraction of the components in the inlet feed had the largest influence on the morphology of the final dried agglomerate.
31981704	5	57	theme	drying	983:988	arg1	method					1005:1010	a method	1003:1010	a method of producing an agglomerated blend of crystalline co-spray dried components leading to possible opportunities for process intensification with the reduction of process steps such as blending, agglomeration, drying and milling into one single stage	1003:1258	The use of crystallo-co-spray drying can serve as a method of producing an agglomerated blend of crystalline co-spray dried components leading to possible opportunities for process intensification with the reduction of process steps such as blending, agglomeration, drying and milling into one single stage.
31981704	5	57	theme	drying	983:988	arg1	use					957:959	The use	953:959	The use of crystallo-co-spray drying	953:988	The use of crystallo-co-spray drying can serve as a method of producing an agglomerated blend of crystalline co-spray dried components leading to possible opportunities for process intensification with the reduction of process steps such as blending, agglomeration, drying and milling into one single stage.
31981704	1	58	theme	α-lactose	235:243	arg1	monohydrate					245:255	α-lactose monohydrate	235:255	α-lactose monohydrate	235:255	The novel process of crystallo-co-spray drying of paracetamol and α-lactose monohydrate was investigated by varying the spray dryer inlet temperature and inlet feed solvent composition.
31981704	0	59	theme	drug/excipient	64:77	arg1	agglomerates					79:90	drug/excipient agglomerates	64:90	drug/excipient agglomerates	64:90	Crystallo-co-spray drying as a new approach to manufacturing of drug/excipient agglomerates: Impact of processing on the properties of paracetamol and lactose mixtures.
31981704	0	60	from	Impact	93:98	arg1	properties					121:130	the properties	117:130	the properties of paracetamol and lactose mixtures	117:166	Crystallo-co-spray drying as a new approach to manufacturing of drug/excipient agglomerates: Impact of processing on the properties of paracetamol and lactose mixtures.
31981704	0	61	dep	drying	19:24	arg1	Impact					93:98	Impact	93:98	Crystallo-co-spray drying as a new approach to manufacturing of drug/excipient agglomerates: Impact of processing on the properties of paracetamol and lactose mixtures.	0:167	Crystallo-co-spray drying as a new approach to manufacturing of drug/excipient agglomerates: Impact of processing on the properties of paracetamol and lactose mixtures.
31981704	4	62	from	increase	827:834	arg1	coating					859:865	paracetamol surface coating	839:865	paracetamol surface coating	839:865	Low excipient soluble fraction resulted in an increase in paracetamol surface coating and high excipient soluble fraction produced agglomerates of highly mixed components.
31981704	0	63	dep	paracetamol	135:145	arg1	mixtures					159:166	mixtures	159:166	mixtures	159:166	Crystallo-co-spray drying as a new approach to manufacturing of drug/excipient agglomerates: Impact of processing on the properties of paracetamol and lactose mixtures.
31981704	1	64	theme	monohydrate	245:255	arg1	drying					209:214	crystallo-co-spray drying	190:214	crystallo-co-spray drying of paracetamol and α-lactose monohydrate	190:255	The novel process of crystallo-co-spray drying of paracetamol and α-lactose monohydrate was investigated by varying the spray dryer inlet temperature and inlet feed solvent composition.
31981704	2	65	theme	hydroxyl	529:536	arg1	paracetamol					615:625	the paracetamol	611:625	the paracetamol detected	611:634	A crystalline agglomerate was obtained with no change to the physical structure of either component throughout both investigations and with possible interactions between the hydroxyl groups of the α-lactose monohydrate and the amide and hydroxyl groups of the paracetamol detected.
31981704	2	65	theme	hydroxyl	529:536	arg1	groups					538:543	the hydroxyl groups	525:543	the hydroxyl groups of the α-lactose monohydrate	525:572	A crystalline agglomerate was obtained with no change to the physical structure of either component throughout both investigations and with possible interactions between the hydroxyl groups of the α-lactose monohydrate and the amide and hydroxyl groups of the paracetamol detected.
31981704	2	65	theme	hydroxyl	529:536	arg1	monohydrate					562:572	the α-lactose monohydrate	548:572	the α-lactose monohydrate	548:572	A crystalline agglomerate was obtained with no change to the physical structure of either component throughout both investigations and with possible interactions between the hydroxyl groups of the α-lactose monohydrate and the amide and hydroxyl groups of the paracetamol detected.
31981704	3	66	theme	soluble	652:658	arg1	fraction					660:667	The percentage soluble fraction	637:667	The percentage soluble fraction of the components in the inlet feed	637:703	The percentage soluble fraction of the components in the inlet feed had the largest influence on the morphology of the final dried agglomerate.
31981704	3	67	from	components	676:685	arg1	feed					700:703	the inlet feed	690:703	the inlet feed	690:703	The percentage soluble fraction of the components in the inlet feed had the largest influence on the morphology of the final dried agglomerate.
33115649	4	0	dep	concentrations	763:776	arg1	25					783:784	25	783:784	25	783:784	Its favorable rheological behaviors were evident on relatively higher concentrations (20, 25, and 30 mg/mL) and moderately lower pH levels (3 and 5) of HOS.
33115649	4	0	dep	concentrations	763:776	arg1	30 mg/mL					791:798	30 mg/mL	791:798	30 mg/mL	791:798	Its favorable rheological behaviors were evident on relatively higher concentrations (20, 25, and 30 mg/mL) and moderately lower pH levels (3 and 5) of HOS.
33115649	4	0	dep	concentrations	763:776	arg1	20					779:780	20	779:780	20	779:780	Its favorable rheological behaviors were evident on relatively higher concentrations (20, 25, and 30 mg/mL) and moderately lower pH levels (3 and 5) of HOS.
33115649	5	1	theme	feed	935:938	arg1	dose					912:915	a high dose	905:915	a high dose of HOS (450 mg/kg feed)	905:939	The anti-fatigue experiments in vivo demonstrated that a high dose of HOS (450 mg/kg feed) prolonged the exhaustive swimming time of mice, significantly induced an increase in blood glucose and glycogen, and decreased lactic acid and serum urea nitrogen levels.
33115649	6	2	dep	conducive	1145:1153	arg1	compared					1252:1259	compared	1252:1259	compared with the conventional herbal supplement Panax quinquefolium	1252:1319	HOS digestion in vivo was fairly conducive to the improvement of energy storage capacity and renal function for physically induced fatigue, compared with the conventional herbal supplement Panax quinquefolium.
33115649	1	3	theme	tropical	223:230	arg1	regions					248:254	tropical and subtropical regions	223:254	tropical and subtropical regions	223:254	Okra, Abelmoschus esculentus (L.) Moench, an annual herbaceous plant, is widely distributed in tropical and subtropical regions.
33115649	0	4	theme	okra	84:87	arg1	Composition					0:10	Composition	0:10	Composition	0:10	Composition, physicochemical properties, and anti-fatigue activity of water-soluble okra (Abelmoschus esculentus) stem pectins.
33115649	0	4	theme	okra	84:87	arg1	properties					29:38	physicochemical properties	13:38	physicochemical properties	13:38	Composition, physicochemical properties, and anti-fatigue activity of water-soluble okra (Abelmoschus esculentus) stem pectins.
33115649	0	4	theme	okra	84:87	arg1	pectins					119:125	stem pectins	114:125	stem pectins	114:125	Composition, physicochemical properties, and anti-fatigue activity of water-soluble okra (Abelmoschus esculentus) stem pectins.
33115649	0	4	theme	okra	84:87	arg1	activity					58:65	anti-fatigue activity	45:65	anti-fatigue activity	45:65	Composition, physicochemical properties, and anti-fatigue activity of water-soluble okra (Abelmoschus esculentus) stem pectins.
33115649	3	5	contain	contained	524:532	arg2	monosaccharides					659:673	other monosaccharides	653:673	other monosaccharides (6.1%)	653:680	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	3	5	contain	contained	524:532	arg2	acid					547:550	galacturonic acid	534:550	galacturonic acid (34%)	534:556	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	3	5	contain	contained	524:532	arg2	acid					621:624	glucuronic acid	610:624	glucuronic acid (2.5%)	610:631	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	3	5	contain	contained	524:532	arg2	xylose					634:639	xylose	634:639	xylose (1.2%)	634:646	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	3	5	contain	contained	524:532	arg2	%					630:630	2.5%	627:630	2.5%	627:630	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	3	5	contain	contained	524:532	arg2	%					606:606	4.2%	603:606	4.2%	603:606	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	3	5	contain	contained	524:532	arg2	%					645:645	1.2%	642:645	1.2%	642:645	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	3	5	contain	contained	524:532	arg1	composition					391:401	The sugar composition	381:401	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02	381:522	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	3	5	contain	contained	524:532	arg2	%					679:679	6.1%	676:679	6.1%	676:679	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	3	5	contain	contained	524:532	arg2	%					555:555	34%	553:555	34%	553:555	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	3	5	contain	contained	524:532	arg2	%					572:572	31%	570:572	31%	570:572	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	3	5	contain	contained	524:532	arg2	galactose					559:567	galactose	559:567	galactose (31%)	559:573	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	3	5	contain	contained	524:532	arg2	%					588:588	21%	586:588	21%	586:588	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	3	5	contain	contained	524:532	arg2	rhamnose					576:583	rhamnose	576:583	rhamnose (21%)	576:589	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	3	5	contain	contained	524:532	arg2	arabinose					592:600	arabinose	592:600	arabinose (4.2%)	592:607	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	1	6	theme	L.	158:159	arg1	plant					191:195	an annual herbaceous plant	170:195	an annual herbaceous plant	170:195	Okra, Abelmoschus esculentus (L.) Moench, an annual herbaceous plant, is widely distributed in tropical and subtropical regions.
33115649	1	6	theme	L.	158:159	arg1	Moench					162:167	Okra, Abelmoschus esculentus (L.) Moench	128:167	Moench	162:167	Okra, Abelmoschus esculentus (L.) Moench, an annual herbaceous plant, is widely distributed in tropical and subtropical regions.
33115649	1	6	theme	L.	158:159	arg1	Abelmoschus					134:144	Abelmoschus	134:144	Abelmoschus	134:144	Okra, Abelmoschus esculentus (L.) Moench, an annual herbaceous plant, is widely distributed in tropical and subtropical regions.
33115649	7	7	theme	stem	1384:1387	arg1	waste					1389:1393	okra stem waste	1379:1393	okra stem waste	1379:1393	Accordingly, HOS exhibits potential for reutilization of okra stem waste.
33115649	3	8	theme	glucuronic	610:619	arg1	acid					621:624	glucuronic acid	610:624	glucuronic acid (2.5%)	610:631	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	3	8	theme	glucuronic	610:619	arg1	%					630:630	2.5%	627:630	2.5%	627:630	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	3	9	theme	1.02 ± 0.02	512:522	arg1	weight					456:461	an weight-average molecular weight	428:461	an weight-average molecular weight of 178.4 ± 2.1 kDa	428:480	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	3	9	theme	1.02 ± 0.02	512:522	arg1	index					503:507	a polydispersity index	486:507	a polydispersity index of 1.02 ± 0.02	486:522	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	2	10	from	stems	302:306	arg1	hydrocolloids					278:290	Water-soluble pectic hydrocolloids	257:290	Water-soluble pectic hydrocolloids from okra stems (HOS)	257:312	Water-soluble pectic hydrocolloids from okra stems (HOS) were extracted and purified using polydivinylbenzene HP-20 resins.
33115649	1	11	theme	subtropical	236:246	arg1	regions					248:254	tropical and subtropical regions	223:254	tropical and subtropical regions	223:254	Okra, Abelmoschus esculentus (L.) Moench, an annual herbaceous plant, is widely distributed in tropical and subtropical regions.
33115649	2	12	theme	pectic	271:276	arg1	hydrocolloids					278:290	Water-soluble pectic hydrocolloids	257:290	Water-soluble pectic hydrocolloids from okra stems (HOS)	257:312	Water-soluble pectic hydrocolloids from okra stems (HOS) were extracted and purified using polydivinylbenzene HP-20 resins.
33115649	0	13	theme	stem	114:117	arg1	pectins					119:125	stem pectins	114:125	stem pectins	114:125	Composition, physicochemical properties, and anti-fatigue activity of water-soluble okra (Abelmoschus esculentus) stem pectins.
33115649	0	13	theme	stem	114:117	arg1	Composition					0:10	Composition	0:10	Composition	0:10	Composition, physicochemical properties, and anti-fatigue activity of water-soluble okra (Abelmoschus esculentus) stem pectins.
33115649	0	13	theme	stem	114:117	arg1	activity					58:65	anti-fatigue activity	45:65	anti-fatigue activity	45:65	Composition, physicochemical properties, and anti-fatigue activity of water-soluble okra (Abelmoschus esculentus) stem pectins.
33115649	4	14	theme	favorable	697:705	arg1	behaviors					719:727	Its favorable rheological behaviors	693:727	Its favorable rheological behaviors	693:727	Its favorable rheological behaviors were evident on relatively higher concentrations (20, 25, and 30 mg/mL) and moderately lower pH levels (3 and 5) of HOS.
33115649	2	15	theme	Water-soluble	257:269	arg1	hydrocolloids					278:290	Water-soluble pectic hydrocolloids	257:290	Water-soluble pectic hydrocolloids from okra stems (HOS)	257:312	Water-soluble pectic hydrocolloids from okra stems (HOS) were extracted and purified using polydivinylbenzene HP-20 resins.
33115649	6	16	theme	herbal	1283:1288	arg1	quinquefolium					1307:1319	the conventional herbal supplement Panax quinquefolium	1266:1319	the conventional herbal supplement Panax quinquefolium	1266:1319	HOS digestion in vivo was fairly conducive to the improvement of energy storage capacity and renal function for physically induced fatigue, compared with the conventional herbal supplement Panax quinquefolium.
33115649	6	17	theme	capacity	1192:1199	arg1	improvement					1162:1172	the improvement	1158:1172	the improvement of energy storage capacity and renal function for physically induced fatigue	1158:1249	HOS digestion in vivo was fairly conducive to the improvement of energy storage capacity and renal function for physically induced fatigue, compared with the conventional herbal supplement Panax quinquefolium.
33115649	5	18	theme	HOS	920:922	arg1	feed					935:938	HOS (450 mg/kg feed)	920:939	HOS (450 mg/kg feed)	920:939	The anti-fatigue experiments in vivo demonstrated that a high dose of HOS (450 mg/kg feed) prolonged the exhaustive swimming time of mice, significantly induced an increase in blood glucose and glycogen, and decreased lactic acid and serum urea nitrogen levels.
33115649	5	19	dep	in	879:880	arg1	vivo					882:885	vivo	882:885	vivo	882:885	The anti-fatigue experiments in vivo demonstrated that a high dose of HOS (450 mg/kg feed) prolonged the exhaustive swimming time of mice, significantly induced an increase in blood glucose and glycogen, and decreased lactic acid and serum urea nitrogen levels.
33115649	7	20	theme	okra	1379:1382	arg1	waste					1389:1393	okra stem waste	1379:1393	okra stem waste	1379:1393	Accordingly, HOS exhibits potential for reutilization of okra stem waste.
33115649	4	21	theme	HOS	845:847	arg1	levels					825:830	moderately lower pH levels	805:830	moderately lower pH levels (3 and 5)	805:840	Its favorable rheological behaviors were evident on relatively higher concentrations (20, 25, and 30 mg/mL) and moderately lower pH levels (3 and 5) of HOS.
33115649	4	21	theme	HOS	845:847	arg1	concentrations					763:776	relatively higher concentrations	745:776	relatively higher concentrations (20, 25, and 30 mg/mL)	745:799	Its favorable rheological behaviors were evident on relatively higher concentrations (20, 25, and 30 mg/mL) and moderately lower pH levels (3 and 5) of HOS.
33115649	4	22	theme	pH	822:823	arg1	levels					825:830	moderately lower pH levels	805:830	moderately lower pH levels (3 and 5)	805:840	Its favorable rheological behaviors were evident on relatively higher concentrations (20, 25, and 30 mg/mL) and moderately lower pH levels (3 and 5) of HOS.
33115649	4	23	theme	higher	756:761	arg1	concentrations					763:776	relatively higher concentrations	745:776	relatively higher concentrations (20, 25, and 30 mg/mL)	745:799	Its favorable rheological behaviors were evident on relatively higher concentrations (20, 25, and 30 mg/mL) and moderately lower pH levels (3 and 5) of HOS.
33115649	3	24	theme	purified	410:417	arg1	HOS					419:421	the purified HOS	406:421	the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02	406:522	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	6	25	theme	induced	1235:1241	arg1	fatigue					1243:1249	physically induced fatigue	1224:1249	physically induced fatigue	1224:1249	HOS digestion in vivo was fairly conducive to the improvement of energy storage capacity and renal function for physically induced fatigue, compared with the conventional herbal supplement Panax quinquefolium.
33115649	4	26	theme	rheological	707:717	arg1	behaviors					719:727	Its favorable rheological behaviors	693:727	Its favorable rheological behaviors	693:727	Its favorable rheological behaviors were evident on relatively higher concentrations (20, 25, and 30 mg/mL) and moderately lower pH levels (3 and 5) of HOS.
33115649	0	27	dep	okra	84:87	arg1	esculentus					102:111	Abelmoschus esculentus	90:111	Abelmoschus esculentus	90:111	Composition, physicochemical properties, and anti-fatigue activity of water-soluble okra (Abelmoschus esculentus) stem pectins.
33115649	5	28	theme	nitrogen	1095:1102	arg1	levels					1104:1109	lactic acid and serum urea nitrogen levels	1068:1109	lactic acid and serum urea nitrogen levels	1068:1109	The anti-fatigue experiments in vivo demonstrated that a high dose of HOS (450 mg/kg feed) prolonged the exhaustive swimming time of mice, significantly induced an increase in blood glucose and glycogen, and decreased lactic acid and serum urea nitrogen levels.
33115649	2	29	theme	HP-20	367:371	arg1	resins					373:378	polydivinylbenzene HP-20 resins	348:378	polydivinylbenzene HP-20 resins	348:378	Water-soluble pectic hydrocolloids from okra stems (HOS) were extracted and purified using polydivinylbenzene HP-20 resins.
33115649	5	30	theme	mice	983:986	arg1	time					975:978	the exhaustive swimming time	951:978	the exhaustive swimming time of mice	951:986	The anti-fatigue experiments in vivo demonstrated that a high dose of HOS (450 mg/kg feed) prolonged the exhaustive swimming time of mice, significantly induced an increase in blood glucose and glycogen, and decreased lactic acid and serum urea nitrogen levels.
33115649	5	31	from	increase	1014:1021	arg1	glucose					1032:1038	blood glucose	1026:1038	blood glucose	1026:1038	The anti-fatigue experiments in vivo demonstrated that a high dose of HOS (450 mg/kg feed) prolonged the exhaustive swimming time of mice, significantly induced an increase in blood glucose and glycogen, and decreased lactic acid and serum urea nitrogen levels.
33115649	5	31	from	increase	1014:1021	arg1	glycogen					1044:1051	glycogen	1044:1051	glycogen	1044:1051	The anti-fatigue experiments in vivo demonstrated that a high dose of HOS (450 mg/kg feed) prolonged the exhaustive swimming time of mice, significantly induced an increase in blood glucose and glycogen, and decreased lactic acid and serum urea nitrogen levels.
33115649	2	32	theme	okra	297:300	arg1	HOS					309:311	HOS	309:311	HOS	309:311	Water-soluble pectic hydrocolloids from okra stems (HOS) were extracted and purified using polydivinylbenzene HP-20 resins.
33115649	2	32	theme	okra	297:300	arg1	stems					302:306	okra stems	297:306	okra stems (HOS)	297:312	Water-soluble pectic hydrocolloids from okra stems (HOS) were extracted and purified using polydivinylbenzene HP-20 resins.
33115649	1	33	theme	annual	173:178	arg1	plant					191:195	an annual herbaceous plant	170:195	an annual herbaceous plant	170:195	Okra, Abelmoschus esculentus (L.) Moench, an annual herbaceous plant, is widely distributed in tropical and subtropical regions.
33115649	1	33	theme	annual	173:178	arg1	Moench					162:167	Okra, Abelmoschus esculentus (L.) Moench	128:167	Moench	162:167	Okra, Abelmoschus esculentus (L.) Moench, an annual herbaceous plant, is widely distributed in tropical and subtropical regions.
33115649	0	34	theme	physicochemical	13:27	arg1	properties					29:38	physicochemical properties	13:38	physicochemical properties	13:38	Composition, physicochemical properties, and anti-fatigue activity of water-soluble okra (Abelmoschus esculentus) stem pectins.
33115649	3	35	theme	galacturonic	534:545	arg1	acid					547:550	galacturonic acid	534:550	galacturonic acid (34%)	534:556	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	3	35	theme	galacturonic	534:545	arg1	%					555:555	34%	553:555	34%	553:555	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	0	36	dep	pectins	119:125	arg1	Composition					0:10	Composition	0:10	Composition	0:10	Composition, physicochemical properties, and anti-fatigue activity of water-soluble okra (Abelmoschus esculentus) stem pectins.
33115649	0	36	dep	pectins	119:125	arg1	properties					29:38	physicochemical properties	13:38	physicochemical properties	13:38	Composition, physicochemical properties, and anti-fatigue activity of water-soluble okra (Abelmoschus esculentus) stem pectins.
33115649	0	36	dep	pectins	119:125	arg1	pectins					119:125	stem pectins	114:125	stem pectins	114:125	Composition, physicochemical properties, and anti-fatigue activity of water-soluble okra (Abelmoschus esculentus) stem pectins.
33115649	0	36	dep	pectins	119:125	arg1	activity					58:65	anti-fatigue activity	45:65	anti-fatigue activity	45:65	Composition, physicochemical properties, and anti-fatigue activity of water-soluble okra (Abelmoschus esculentus) stem pectins.
33115649	1	37	theme	herbaceous	180:189	arg1	plant					191:195	an annual herbaceous plant	170:195	an annual herbaceous plant	170:195	Okra, Abelmoschus esculentus (L.) Moench, an annual herbaceous plant, is widely distributed in tropical and subtropical regions.
33115649	1	37	theme	herbaceous	180:189	arg1	Moench					162:167	Okra, Abelmoschus esculentus (L.) Moench	128:167	Moench	162:167	Okra, Abelmoschus esculentus (L.) Moench, an annual herbaceous plant, is widely distributed in tropical and subtropical regions.
33115649	6	38	theme	function	1211:1218	arg1	improvement					1162:1172	the improvement	1158:1172	the improvement of energy storage capacity and renal function for physically induced fatigue	1158:1249	HOS digestion in vivo was fairly conducive to the improvement of energy storage capacity and renal function for physically induced fatigue, compared with the conventional herbal supplement Panax quinquefolium.
33115649	3	39	theme	HOS	419:421	arg1	composition					391:401	The sugar composition	381:401	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02	381:522	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	3	40	theme	weight-average	431:444	arg1	weight					456:461	an weight-average molecular weight	428:461	an weight-average molecular weight of 178.4 ± 2.1 kDa	428:480	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	6	41	theme	Panax	1301:1305	arg1	quinquefolium					1307:1319	the conventional herbal supplement Panax quinquefolium	1266:1319	the conventional herbal supplement Panax quinquefolium	1266:1319	HOS digestion in vivo was fairly conducive to the improvement of energy storage capacity and renal function for physically induced fatigue, compared with the conventional herbal supplement Panax quinquefolium.
33115649	6	42	theme	energy	1177:1182	arg1	capacity					1192:1199	energy storage capacity	1177:1199	energy storage capacity	1177:1199	HOS digestion in vivo was fairly conducive to the improvement of energy storage capacity and renal function for physically induced fatigue, compared with the conventional herbal supplement Panax quinquefolium.
33115649	6	43	theme	renal	1205:1209	arg1	function					1211:1218	renal function	1205:1218	renal function	1205:1218	HOS digestion in vivo was fairly conducive to the improvement of energy storage capacity and renal function for physically induced fatigue, compared with the conventional herbal supplement Panax quinquefolium.
33115649	3	44	theme	molecular	446:454	arg1	weight					456:461	an weight-average molecular weight	428:461	an weight-average molecular weight of 178.4 ± 2.1 kDa	428:480	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	3	45	theme	sugar	385:389	arg1	composition					391:401	The sugar composition	381:401	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02	381:522	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	3	46	theme	other	653:657	arg1	monosaccharides					659:673	other monosaccharides	653:673	other monosaccharides (6.1%)	653:680	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	3	46	theme	other	653:657	arg1	%					679:679	6.1%	676:679	6.1%	676:679	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	6	47	theme	HOS	1112:1114	arg1	digestion					1116:1124	HOS digestion	1112:1124	HOS digestion in vivo	1112:1132	HOS digestion in vivo was fairly conducive to the improvement of energy storage capacity and renal function for physically induced fatigue, compared with the conventional herbal supplement Panax quinquefolium.
33115649	5	48	theme	high	907:910	arg1	dose					912:915	a high dose	905:915	a high dose of HOS (450 mg/kg feed)	905:939	The anti-fatigue experiments in vivo demonstrated that a high dose of HOS (450 mg/kg feed) prolonged the exhaustive swimming time of mice, significantly induced an increase in blood glucose and glycogen, and decreased lactic acid and serum urea nitrogen levels.
33115649	0	49	theme	anti-fatigue	45:56	arg1	pectins					119:125	stem pectins	114:125	stem pectins	114:125	Composition, physicochemical properties, and anti-fatigue activity of water-soluble okra (Abelmoschus esculentus) stem pectins.
33115649	0	49	theme	anti-fatigue	45:56	arg1	activity					58:65	anti-fatigue activity	45:65	anti-fatigue activity	45:65	Composition, physicochemical properties, and anti-fatigue activity of water-soluble okra (Abelmoschus esculentus) stem pectins.
33115649	5	50	theme	swimming	966:973	arg1	time					975:978	the exhaustive swimming time	951:978	the exhaustive swimming time of mice	951:986	The anti-fatigue experiments in vivo demonstrated that a high dose of HOS (450 mg/kg feed) prolonged the exhaustive swimming time of mice, significantly induced an increase in blood glucose and glycogen, and decreased lactic acid and serum urea nitrogen levels.
33115649	3	51	with	HOS	419:421	arg1	weight					456:461	an weight-average molecular weight	428:461	an weight-average molecular weight of 178.4 ± 2.1 kDa	428:480	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	3	51	with	HOS	419:421	arg1	index					503:507	a polydispersity index	486:507	a polydispersity index of 1.02 ± 0.02	486:522	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	5	52	theme	anti-fatigue	854:865	arg1	experiments					867:877	The anti-fatigue experiments	850:877	The anti-fatigue experiments in vivo	850:885	The anti-fatigue experiments in vivo demonstrated that a high dose of HOS (450 mg/kg feed) prolonged the exhaustive swimming time of mice, significantly induced an increase in blood glucose and glycogen, and decreased lactic acid and serum urea nitrogen levels.
33115649	6	53	theme	conventional	1270:1281	arg1	quinquefolium					1307:1319	the conventional herbal supplement Panax quinquefolium	1266:1319	the conventional herbal supplement Panax quinquefolium	1266:1319	HOS digestion in vivo was fairly conducive to the improvement of energy storage capacity and renal function for physically induced fatigue, compared with the conventional herbal supplement Panax quinquefolium.
33115649	3	54	theme	polydispersity	488:501	arg1	index					503:507	a polydispersity index	486:507	a polydispersity index of 1.02 ± 0.02	486:522	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	5	55	theme	acid	1075:1078	arg1	levels					1104:1109	lactic acid and serum urea nitrogen levels	1068:1109	lactic acid and serum urea nitrogen levels	1068:1109	The anti-fatigue experiments in vivo demonstrated that a high dose of HOS (450 mg/kg feed) prolonged the exhaustive swimming time of mice, significantly induced an increase in blood glucose and glycogen, and decreased lactic acid and serum urea nitrogen levels.
33115649	1	56	theme	Okra	128:131	arg1	plant					191:195	an annual herbaceous plant	170:195	an annual herbaceous plant	170:195	Okra, Abelmoschus esculentus (L.) Moench, an annual herbaceous plant, is widely distributed in tropical and subtropical regions.
33115649	1	56	theme	Okra	128:131	arg1	Moench					162:167	Okra, Abelmoschus esculentus (L.) Moench	128:167	Moench	162:167	Okra, Abelmoschus esculentus (L.) Moench, an annual herbaceous plant, is widely distributed in tropical and subtropical regions.
33115649	1	56	theme	Okra	128:131	arg1	Abelmoschus					134:144	Abelmoschus	134:144	Abelmoschus	134:144	Okra, Abelmoschus esculentus (L.) Moench, an annual herbaceous plant, is widely distributed in tropical and subtropical regions.
33115649	6	57	theme	supplement	1290:1299	arg1	quinquefolium					1307:1319	the conventional herbal supplement Panax quinquefolium	1266:1319	the conventional herbal supplement Panax quinquefolium	1266:1319	HOS digestion in vivo was fairly conducive to the improvement of energy storage capacity and renal function for physically induced fatigue, compared with the conventional herbal supplement Panax quinquefolium.
33115649	5	58	theme	blood	1026:1030	arg1	glucose					1032:1038	blood glucose	1026:1038	blood glucose	1026:1038	The anti-fatigue experiments in vivo demonstrated that a high dose of HOS (450 mg/kg feed) prolonged the exhaustive swimming time of mice, significantly induced an increase in blood glucose and glycogen, and decreased lactic acid and serum urea nitrogen levels.
33115649	5	59	theme	in	879:880	arg1	experiments					867:877	The anti-fatigue experiments	850:877	The anti-fatigue experiments in vivo	850:885	The anti-fatigue experiments in vivo demonstrated that a high dose of HOS (450 mg/kg feed) prolonged the exhaustive swimming time of mice, significantly induced an increase in blood glucose and glycogen, and decreased lactic acid and serum urea nitrogen levels.
33115649	6	60	theme	storage	1184:1190	arg1	capacity					1192:1199	energy storage capacity	1177:1199	energy storage capacity	1177:1199	HOS digestion in vivo was fairly conducive to the improvement of energy storage capacity and renal function for physically induced fatigue, compared with the conventional herbal supplement Panax quinquefolium.
33115649	4	61	dep	levels	825:830	arg1	5					839:839	5	839:839	5	839:839	Its favorable rheological behaviors were evident on relatively higher concentrations (20, 25, and 30 mg/mL) and moderately lower pH levels (3 and 5) of HOS.
33115649	4	61	dep	levels	825:830	arg1	3					833:833	3	833:833	3	833:833	Its favorable rheological behaviors were evident on relatively higher concentrations (20, 25, and 30 mg/mL) and moderately lower pH levels (3 and 5) of HOS.
33115649	5	62	theme	serum	1084:1088	arg1	nitrogen					1095:1102	serum urea nitrogen	1084:1102	serum urea nitrogen	1084:1102	The anti-fatigue experiments in vivo demonstrated that a high dose of HOS (450 mg/kg feed) prolonged the exhaustive swimming time of mice, significantly induced an increase in blood glucose and glycogen, and decreased lactic acid and serum urea nitrogen levels.
33115649	3	63	theme	178.4 ± 2.1 kDa	466:480	arg1	weight					456:461	an weight-average molecular weight	428:461	an weight-average molecular weight of 178.4 ± 2.1 kDa	428:480	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	3	63	theme	178.4 ± 2.1 kDa	466:480	arg1	index					503:507	a polydispersity index	486:507	a polydispersity index of 1.02 ± 0.02	486:522	The sugar composition of the purified HOS with an weight-average molecular weight of 178.4 ± 2.1 kDa and a polydispersity index of 1.02 ± 0.02 contained galacturonic acid (34%), galactose (31%), rhamnose (21%), arabinose (4.2%), glucuronic acid (2.5%), xylose (1.2%), and other monosaccharides (6.1%) by weight.
33115649	0	64	theme	water-soluble	70:82	arg1	okra					84:87	water-soluble okra	70:87	water-soluble okra (Abelmoschus esculentus)	70:112	Composition, physicochemical properties, and anti-fatigue activity of water-soluble okra (Abelmoschus esculentus) stem pectins.
33115649	5	65	theme	lactic	1068:1073	arg1	acid					1075:1078	lactic acid	1068:1078	lactic acid	1068:1078	The anti-fatigue experiments in vivo demonstrated that a high dose of HOS (450 mg/kg feed) prolonged the exhaustive swimming time of mice, significantly induced an increase in blood glucose and glycogen, and decreased lactic acid and serum urea nitrogen levels.
33115649	2	66	theme	polydivinylbenzene	348:365	arg1	resins					373:378	polydivinylbenzene HP-20 resins	348:378	polydivinylbenzene HP-20 resins	348:378	Water-soluble pectic hydrocolloids from okra stems (HOS) were extracted and purified using polydivinylbenzene HP-20 resins.
33115649	5	67	theme	urea	1090:1093	arg1	nitrogen					1095:1102	serum urea nitrogen	1084:1102	serum urea nitrogen	1084:1102	The anti-fatigue experiments in vivo demonstrated that a high dose of HOS (450 mg/kg feed) prolonged the exhaustive swimming time of mice, significantly induced an increase in blood glucose and glycogen, and decreased lactic acid and serum urea nitrogen levels.
33115649	7	68	theme	waste	1389:1393	arg1	reutilization					1362:1374	reutilization	1362:1374	reutilization of okra stem waste	1362:1393	Accordingly, HOS exhibits potential for reutilization of okra stem waste.
33115649	4	69	theme	lower	816:820	arg1	levels					825:830	moderately lower pH levels	805:830	moderately lower pH levels (3 and 5)	805:840	Its favorable rheological behaviors were evident on relatively higher concentrations (20, 25, and 30 mg/mL) and moderately lower pH levels (3 and 5) of HOS.
33115649	5	70	theme	450 mg/kg	925:933	arg1	feed					935:938	HOS (450 mg/kg feed)	920:939	HOS (450 mg/kg feed)	920:939	The anti-fatigue experiments in vivo demonstrated that a high dose of HOS (450 mg/kg feed) prolonged the exhaustive swimming time of mice, significantly induced an increase in blood glucose and glycogen, and decreased lactic acid and serum urea nitrogen levels.
33115649	1	71	theme	esculentus	146:155	arg1	plant					191:195	an annual herbaceous plant	170:195	an annual herbaceous plant	170:195	Okra, Abelmoschus esculentus (L.) Moench, an annual herbaceous plant, is widely distributed in tropical and subtropical regions.
33115649	1	71	theme	esculentus	146:155	arg1	Moench					162:167	Okra, Abelmoschus esculentus (L.) Moench	128:167	Moench	162:167	Okra, Abelmoschus esculentus (L.) Moench, an annual herbaceous plant, is widely distributed in tropical and subtropical regions.
33115649	1	71	theme	esculentus	146:155	arg1	Abelmoschus					134:144	Abelmoschus	134:144	Abelmoschus	134:144	Okra, Abelmoschus esculentus (L.) Moench, an annual herbaceous plant, is widely distributed in tropical and subtropical regions.
33115649	5	72	theme	exhaustive	955:964	arg1	time					975:978	the exhaustive swimming time	951:978	the exhaustive swimming time of mice	951:986	The anti-fatigue experiments in vivo demonstrated that a high dose of HOS (450 mg/kg feed) prolonged the exhaustive swimming time of mice, significantly induced an increase in blood glucose and glycogen, and decreased lactic acid and serum urea nitrogen levels.
33178355	8	0	theme	synthesis	1100:1108	arg1	models					1110:1115	glycan synthesis models	1093:1115	glycan synthesis models	1093:1115	With a clear guide for generating reaction rule descriptions, glycan synthesis models will be more interoperable and reproducible thereby moving glycoinformatics closer to compliance with FAIR standards.
33178355	1	1	with	interactions	199:210	arg1	glycoconjugates					217:231	glycoconjugates	217:231	glycoconjugates	217:231	Systems glycobiology aims to provide models and analysis tools that account for the biosynthesis, regulation, and interactions with glycoconjugates.
33178355	1	2	theme	analysis	133:140	arg1	tools					142:146	analysis tools	133:146	analysis tools	133:146	Systems glycobiology aims to provide models and analysis tools that account for the biosynthesis, regulation, and interactions with glycoconjugates.
33178355	8	3	theme	rule	1074:1077	arg1	descriptions					1079:1090	reaction rule descriptions	1065:1090	reaction rule descriptions	1065:1090	With a clear guide for generating reaction rule descriptions, glycan synthesis models will be more interoperable and reproducible thereby moving glycoinformatics closer to compliance with FAIR standards.
33178355	9	4	dep	present	1244:1250	arg1	Code					1259:1262	Code	1259:1262	present Linear Code for Reaction Rules (LiCoRR), version 1.0, an unambiguous representation for describing glycosylation reactions in both literature and code	1244:1401	Here, we present Linear Code for Reaction Rules (LiCoRR), version 1.0, an unambiguous representation for describing glycosylation reactions in both literature and code.
33178355	9	5	theme	Reaction	1268:1275	arg1	representation					1321:1334	an unambiguous representation	1306:1334	an unambiguous representation for describing glycosylation reactions in both literature and code	1306:1401	Here, we present Linear Code for Reaction Rules (LiCoRR), version 1.0, an unambiguous representation for describing glycosylation reactions in both literature and code.
33178355	9	5	theme	Reaction	1268:1275	arg1	version					1293:1299	version 1.0	1293:1303	version 1.0	1293:1303	Here, we present Linear Code for Reaction Rules (LiCoRR), version 1.0, an unambiguous representation for describing glycosylation reactions in both literature and code.
33178355	9	5	theme	Reaction	1268:1275	arg1	Rules					1277:1281	Reaction Rules	1268:1281	Reaction Rules (LiCoRR)	1268:1290	Here, we present Linear Code for Reaction Rules (LiCoRR), version 1.0, an unambiguous representation for describing glycosylation reactions in both literature and code.
33178355	9	5	theme	Reaction	1268:1275	arg1	LiCoRR					1284:1289	LiCoRR	1284:1289	LiCoRR	1284:1289	Here, we present Linear Code for Reaction Rules (LiCoRR), version 1.0, an unambiguous representation for describing glycosylation reactions in both literature and code.
33178355	7	6	theme	reaction	926:933	arg1	rules					935:939	reaction rules	926:939	reaction rules	926:939	Through this extension and specification of Linear Code to reaction rules, we aim to minimize inconsistent symbology thereby making glycan database queries easier.
33178355	7	7	theme	Linear	911:916	arg1	specification					894:906	specification	894:906	specification	894:906	Through this extension and specification of Linear Code to reaction rules, we aim to minimize inconsistent symbology thereby making glycan database queries easier.
33178355	7	7	theme	Linear	911:916	arg1	extension					880:888	extension	880:888	extension	880:888	Through this extension and specification of Linear Code to reaction rules, we aim to minimize inconsistent symbology thereby making glycan database queries easier.
33178355	3	8	dep	Linear	354:359	arg1	Code					361:364	Code	361:364	Code	361:364	Linear Code, a linearized and readily parsable glycan structure representation, is such a language.
33178355	7	9	dep	Linear	911:916	arg1	Code					918:921	Code	918:921	Code	918:921	Through this extension and specification of Linear Code to reaction rules, we aim to minimize inconsistent symbology thereby making glycan database queries easier.
33178355	6	10	theme	consensus-based	762:776	arg1	extension					778:786	a consensus-based extension	760:786	a consensus-based extension of Linear Code that can be used for reaction rule specification going forward	760:864	We recommend options for a consensus-based extension of Linear Code that can be used for reaction rule specification going forward.
33178355	7	11	dep	extension	880:888	arg1	this					875:878	this	875:878	this	875:878	Through this extension and specification of Linear Code to reaction rules, we aim to minimize inconsistent symbology thereby making glycan database queries easier.
33178355	3	12	theme	glycan	401:406	arg1	Linear					354:359	Linear	354:359	Linear	354:359	Linear Code, a linearized and readily parsable glycan structure representation, is such a language.
33178355	3	12	theme	glycan	401:406	arg1	representation					418:431	a linearized and readily parsable glycan structure representation	367:431	a linearized and readily parsable glycan structure representation	367:431	Linear Code, a linearized and readily parsable glycan structure representation, is such a language.
33178355	3	13	theme	structure	408:416	arg1	Linear					354:359	Linear	354:359	Linear	354:359	Linear Code, a linearized and readily parsable glycan structure representation, is such a language.
33178355	3	13	theme	structure	408:416	arg1	representation					418:431	a linearized and readily parsable glycan structure representation	367:431	a linearized and readily parsable glycan structure representation	367:431	Linear Code, a linearized and readily parsable glycan structure representation, is such a language.
33178355	0	14	theme	consensus-based	2:16	arg1	extension					31:39	A consensus-based and readable extension	0:39	A consensus-based and readable extension of Linear	0:49	A consensus-based and readable extension of Linear Code for Reaction Rules (LiCoRR).
33178355	6	15	theme	rule	833:836	arg1	specification					838:850	reaction rule specification	824:850	reaction rule specification going forward	824:864	We recommend options for a consensus-based extension of Linear Code that can be used for reaction rule specification going forward.
33178355	5	16	dep	consensuses	661:671	arg1	the					657:659	the	657:659	the	657:659	Here, we delineate the consensuses and inconsistencies that have arisen through this adaptation.
33178355	4	17	theme	reaction	508:515	arg1	rules					517:521	reaction rules	508:521	reaction rules	508:521	For this reason, Linear Code was adapted to represent reaction rules, but the syntax has drifted from its original description to accommodate new and originally unforeseen challenges.
33178355	4	18	theme	unforeseen	615:624	arg1	challenges					626:635	new and originally unforeseen challenges	596:635	new and originally unforeseen challenges	596:635	For this reason, Linear Code was adapted to represent reaction rules, but the syntax has drifted from its original description to accommodate new and originally unforeseen challenges.
33178355	0	19	theme	readable	22:29	arg1	extension					31:39	A consensus-based and readable extension	0:39	A consensus-based and readable extension of Linear	0:49	A consensus-based and readable extension of Linear Code for Reaction Rules (LiCoRR).
33178355	6	20	dep	Linear	791:796	arg1	Code					798:801	Code	798:801	Code	798:801	We recommend options for a consensus-based extension of Linear Code that can be used for reaction rule specification going forward.
33178355	1	21	with	regulation	183:192	arg1	glycoconjugates					217:231	glycoconjugates	217:231	glycoconjugates	217:231	Systems glycobiology aims to provide models and analysis tools that account for the biosynthesis, regulation, and interactions with glycoconjugates.
33178355	8	22	theme	clear	1038:1042	arg1	guide					1044:1048	a clear guide	1036:1048	a clear guide for generating reaction rule descriptions	1036:1090	With a clear guide for generating reaction rule descriptions, glycan synthesis models will be more interoperable and reproducible thereby moving glycoinformatics closer to compliance with FAIR standards.
33178355	6	23	theme	reaction	824:831	arg1	specification					838:850	reaction rule specification	824:850	reaction rule specification going forward	824:864	We recommend options for a consensus-based extension of Linear Code that can be used for reaction rule specification going forward.
33178355	1	24	theme	Systems	85:91	arg1	glycobiology					93:104	Systems glycobiology	85:104	Systems glycobiology	85:104	Systems glycobiology aims to provide models and analysis tools that account for the biosynthesis, regulation, and interactions with glycoconjugates.
33178355	3	25	theme	linearized	369:378	arg1	Linear					354:359	Linear	354:359	Linear	354:359	Linear Code, a linearized and readily parsable glycan structure representation, is such a language.
33178355	3	25	theme	linearized	369:378	arg1	representation					418:431	a linearized and readily parsable glycan structure representation	367:431	a linearized and readily parsable glycan structure representation	367:431	Linear Code, a linearized and readily parsable glycan structure representation, is such a language.
33178355	1	26	with	biosynthesis	169:180	arg1	glycoconjugates					217:231	glycoconjugates	217:231	glycoconjugates	217:231	Systems glycobiology aims to provide models and analysis tools that account for the biosynthesis, regulation, and interactions with glycoconjugates.
33178355	3	27	theme	parsable	392:399	arg1	Linear					354:359	Linear	354:359	Linear	354:359	Linear Code, a linearized and readily parsable glycan structure representation, is such a language.
33178355	3	27	theme	parsable	392:399	arg1	representation					418:431	a linearized and readily parsable glycan structure representation	367:431	a linearized and readily parsable glycan structure representation	367:431	Linear Code, a linearized and readily parsable glycan structure representation, is such a language.
33178355	4	28	theme	new	596:598	arg1	challenges					626:635	new and originally unforeseen challenges	596:635	new and originally unforeseen challenges	596:635	For this reason, Linear Code was adapted to represent reaction rules, but the syntax has drifted from its original description to accommodate new and originally unforeseen challenges.
33178355	6	29	used	used	815:818	arg2	Linear					791:796	Linear	791:796	Linear	791:796	We recommend options for a consensus-based extension of Linear Code that can be used for reaction rule specification going forward.
33178355	4	30	dep	Linear	471:476	arg1	Code					478:481	Code	478:481	Code	478:481	For this reason, Linear Code was adapted to represent reaction rules, but the syntax has drifted from its original description to accommodate new and originally unforeseen challenges.
33178355	9	31	theme	unambiguous	1309:1319	arg1	representation					1321:1334	an unambiguous representation	1306:1334	an unambiguous representation for describing glycosylation reactions in both literature and code	1306:1401	Here, we present Linear Code for Reaction Rules (LiCoRR), version 1.0, an unambiguous representation for describing glycosylation reactions in both literature and code.
33178355	9	31	theme	unambiguous	1309:1319	arg1	Rules					1277:1281	Reaction Rules	1268:1281	Reaction Rules (LiCoRR)	1268:1290	Here, we present Linear Code for Reaction Rules (LiCoRR), version 1.0, an unambiguous representation for describing glycosylation reactions in both literature and code.
33178355	7	32	theme	database	1006:1013	arg1	queries					1015:1021	glycan database queries	999:1021	glycan database queries	999:1021	Through this extension and specification of Linear Code to reaction rules, we aim to minimize inconsistent symbology thereby making glycan database queries easier.
33178355	2	33	theme	accessible	313:322	arg1	representation					298:311	a clear glycan representation	283:311	a clear glycan representation accessible to both computers and humans	283:351	To facilitate these methods, there is a need for a clear glycan representation accessible to both computers and humans.
33178355	0	34	theme	Linear	44:49	arg1	extension					31:39	A consensus-based and readable extension	0:39	A consensus-based and readable extension of Linear	0:49	A consensus-based and readable extension of Linear Code for Reaction Rules (LiCoRR).
33178355	8	35	theme	reaction	1065:1072	arg1	descriptions					1079:1090	reaction rule descriptions	1065:1090	reaction rule descriptions	1065:1090	With a clear guide for generating reaction rule descriptions, glycan synthesis models will be more interoperable and reproducible thereby moving glycoinformatics closer to compliance with FAIR standards.
33178355	7	36	theme	inconsistent	961:972	arg1	symbology					974:982	inconsistent symbology	961:982	inconsistent symbology	961:982	Through this extension and specification of Linear Code to reaction rules, we aim to minimize inconsistent symbology thereby making glycan database queries easier.
33178355	2	37	theme	glycan	291:296	arg1	representation					298:311	a clear glycan representation	283:311	a clear glycan representation accessible to both computers and humans	283:351	To facilitate these methods, there is a need for a clear glycan representation accessible to both computers and humans.
33178355	9	38	theme	glycosylation	1351:1363	arg1	reactions					1365:1373	glycosylation reactions	1351:1373	glycosylation reactions	1351:1373	Here, we present Linear Code for Reaction Rules (LiCoRR), version 1.0, an unambiguous representation for describing glycosylation reactions in both literature and code.
33178355	7	39	theme	glycan	999:1004	arg1	queries					1015:1021	glycan database queries	999:1021	glycan database queries	999:1021	Through this extension and specification of Linear Code to reaction rules, we aim to minimize inconsistent symbology thereby making glycan database queries easier.
33178355	2	40	theme	clear	285:289	arg1	representation					298:311	a clear glycan representation	283:311	a clear glycan representation accessible to both computers and humans	283:351	To facilitate these methods, there is a need for a clear glycan representation accessible to both computers and humans.
33178355	8	41	with	interoperable	1130:1142	arg1	guide					1044:1048	a clear guide	1036:1048	a clear guide for generating reaction rule descriptions	1036:1090	With a clear guide for generating reaction rule descriptions, glycan synthesis models will be more interoperable and reproducible thereby moving glycoinformatics closer to compliance with FAIR standards.
33178355	0	42	theme	Reaction	60:67	arg1	Rules					69:73	Reaction Rules	60:73	Reaction Rules (LiCoRR)	60:82	A consensus-based and readable extension of Linear Code for Reaction Rules (LiCoRR).
33178355	0	42	theme	Reaction	60:67	arg1	LiCoRR					76:81	LiCoRR	76:81	LiCoRR	76:81	A consensus-based and readable extension of Linear Code for Reaction Rules (LiCoRR).
33178355	6	43	theme	Linear	791:796	arg1	extension					778:786	a consensus-based extension	760:786	a consensus-based extension of Linear Code that can be used for reaction rule specification going forward	760:864	We recommend options for a consensus-based extension of Linear Code that can be used for reaction rule specification going forward.
33178355	8	44	with	compliance	1203:1212	arg1	standards					1224:1232	FAIR standards	1219:1232	FAIR standards	1219:1232	With a clear guide for generating reaction rule descriptions, glycan synthesis models will be more interoperable and reproducible thereby moving glycoinformatics closer to compliance with FAIR standards.
33178355	8	45	theme	FAIR	1219:1222	arg1	standards					1224:1232	FAIR standards	1219:1232	FAIR standards	1219:1232	With a clear guide for generating reaction rule descriptions, glycan synthesis models will be more interoperable and reproducible thereby moving glycoinformatics closer to compliance with FAIR standards.
33178355	4	46	theme	original	560:567	arg1	description					569:579	its original description to accommodate new and originally unforeseen challenges	556:635	its original description to accommodate new and originally unforeseen challenges	556:635	For this reason, Linear Code was adapted to represent reaction rules, but the syntax has drifted from its original description to accommodate new and originally unforeseen challenges.
33178355	8	47	theme	glycan	1093:1098	arg1	models					1110:1115	glycan synthesis models	1093:1115	glycan synthesis models	1093:1115	With a clear guide for generating reaction rule descriptions, glycan synthesis models will be more interoperable and reproducible thereby moving glycoinformatics closer to compliance with FAIR standards.
33524653	4	0	theme	it	1111:1112	arg1	application					1114:1124	it application	1111:1124	it application for early-stage diagnostics, diseases monitoring, and optimization of therapy	1111:1202	Our fabricated electrochemical AbsCA19-9/CS-MWCNT-Fe3O4 immunosensor performed CA19-9 sensing in physiological range and at a very level which suggest it application for early-stage diagnostics, diseases monitoring, and optimization of therapy.
33524653	7	1	theme	affordable	1642:1651	arg1	detection					1687:1695	affordable, rapid, label-free, and sensitive detection	1642:1695	affordable, rapid, label-free, and sensitive detection of a targeted biomarker	1642:1719	We believe that our fabricated sensor can be the plane of a disease's management program due to affordable, rapid, label-free, and sensitive detection of a targeted biomarker.
33524653	6	2	theme	CA19-9	1447:1452	arg1	immunosensor					1454:1465	AbsCA19-9/CS-MWCNT-Fe3O4 electrochemical CA19-9 immunosensor	1406:1465	AbsCA19-9/CS-MWCNT-Fe3O4 electrochemical CA19-9 immunosensor as a potential and affordable alternate of conventional techniques like ELISA	1406:1543	The results of the studies projected AbsCA19-9/CS-MWCNT-Fe3O4 electrochemical CA19-9 immunosensor as a potential and affordable alternate of conventional techniques like ELISA.
33524653	5	3	theme	clinical	1218:1225	arg1	application					1227:1237	the clinical application	1214:1237	the clinical application	1214:1237	To claim the clinical application, our sensor was tested using real samples and sensing performance was validated using enzyme-linked immune-sorbent assay (ELISA).
33524653	3	4	theme	detection	911:919	arg1	sensitivity					839:849	a high sensitivity	832:849	a high sensitivity as (2.55 µA pg-1 cm-1)	832:872	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19-9 and exhibited a high sensitivity as (2.55 µA pg-1 cm-1), a detection limit of 0.163 pg mL-1, detection range from 1.0 pg mL-1 to 100 ng mL-1.
33524653	3	4	theme	detection	911:919	arg1	range					921:925	detection range	911:925	detection range	911:925	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19-9 and exhibited a high sensitivity as (2.55 µA pg-1 cm-1), a detection limit of 0.163 pg mL-1, detection range from 1.0 pg mL-1 to 100 ng mL-1.
33524653	0	5	theme	diagnostics	103:113	arg1	application					115:125	endometriosis diagnostics application	89:125	endometriosis diagnostics application	89:125	Bio-nanocomposite based highly sensitive and label-free electrochemical immunosensor for endometriosis diagnostics application.
33524653	7	6	theme	program	1627:1633	arg1	sensor					1577:1582	our fabricated sensor	1562:1582	our fabricated sensor	1562:1582	We believe that our fabricated sensor can be the plane of a disease's management program due to affordable, rapid, label-free, and sensitive detection of a targeted biomarker.
33524653	7	6	theme	program	1627:1633	arg1	plane					1595:1599	the plane	1591:1599	the plane of a disease's management program due to affordable, rapid, label-free, and sensitive detection of a targeted biomarker	1591:1719	We believe that our fabricated sensor can be the plane of a disease's management program due to affordable, rapid, label-free, and sensitive detection of a targeted biomarker.
33524653	6	7	theme	electrochemical	1431:1445	arg1	immunosensor					1454:1465	AbsCA19-9/CS-MWCNT-Fe3O4 electrochemical CA19-9 immunosensor	1406:1465	AbsCA19-9/CS-MWCNT-Fe3O4 electrochemical CA19-9 immunosensor as a potential and affordable alternate of conventional techniques like ELISA	1406:1543	The results of the studies projected AbsCA19-9/CS-MWCNT-Fe3O4 electrochemical CA19-9 immunosensor as a potential and affordable alternate of conventional techniques like ELISA.
33524653	4	8	theme	therapy	1196:1202	arg1	diseases					1155:1162	diseases	1155:1162	diseases monitoring	1155:1173	Our fabricated electrochemical AbsCA19-9/CS-MWCNT-Fe3O4 immunosensor performed CA19-9 sensing in physiological range and at a very level which suggest it application for early-stage diagnostics, diseases monitoring, and optimization of therapy.
33524653	4	8	theme	therapy	1196:1202	arg1	diagnostics					1142:1152	early-stage diagnostics	1130:1152	early-stage diagnostics	1130:1152	Our fabricated electrochemical AbsCA19-9/CS-MWCNT-Fe3O4 immunosensor performed CA19-9 sensing in physiological range and at a very level which suggest it application for early-stage diagnostics, diseases monitoring, and optimization of therapy.
33524653	4	8	theme	therapy	1196:1202	arg1	optimization					1180:1191	optimization	1180:1191	optimization of therapy	1180:1202	Our fabricated electrochemical AbsCA19-9/CS-MWCNT-Fe3O4 immunosensor performed CA19-9 sensing in physiological range and at a very level which suggest it application for early-stage diagnostics, diseases monitoring, and optimization of therapy.
33524653	1	9	theme	endometriosis	282:294	arg1	application					308:318	endometriosis diagnostics application	282:318	endometriosis diagnostics application	282:318	In this research, for the first time, a bio-nanocomposites based highly sensitive and label-free electrochemical immunosensor is reported with the aim of endometriosis diagnostics application.
33524653	2	10	theme	antigen	604:610	arg1	CA19-9					618:623	CA19-9	618:623	CA19-9	618:623	Multiwalled carbon nanotube and magnetite nanoparticle (MWCNT-Fe3O4) was dispersed in chitosan (CS) to fabricate a bio-nanocomposite to immobilize very monoclonal specific antibody (via cross-linking using glutaraldehyde) for selective electrochemical immuno-sensing of carbohydrate antigen 19-9 (CA19-9), a potential biomarker for endometriosis diagnostics.
33524653	2	10	theme	antigen	604:610	arg1	19-9					612:615	carbohydrate antigen 19-9	591:615	carbohydrate antigen 19-9 (CA19-9)	591:624	Multiwalled carbon nanotube and magnetite nanoparticle (MWCNT-Fe3O4) was dispersed in chitosan (CS) to fabricate a bio-nanocomposite to immobilize very monoclonal specific antibody (via cross-linking using glutaraldehyde) for selective electrochemical immuno-sensing of carbohydrate antigen 19-9 (CA19-9), a potential biomarker for endometriosis diagnostics.
33524653	2	10	theme	antigen	604:610	arg1	biomarker					639:647	a potential biomarker	627:647	a potential biomarker for endometriosis diagnostics	627:677	Multiwalled carbon nanotube and magnetite nanoparticle (MWCNT-Fe3O4) was dispersed in chitosan (CS) to fabricate a bio-nanocomposite to immobilize very monoclonal specific antibody (via cross-linking using glutaraldehyde) for selective electrochemical immuno-sensing of carbohydrate antigen 19-9 (CA19-9), a potential biomarker for endometriosis diagnostics.
33524653	3	11	theme	CA	810:811	arg1	19-9					813:816	CA 19-9	810:816	CA 19-9	810:816	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19-9 and exhibited a high sensitivity as (2.55 µA pg-1 cm-1), a detection limit of 0.163 pg mL-1, detection range from 1.0 pg mL-1 to 100 ng mL-1.
33524653	2	12	theme	endometriosis	653:665	arg1	diagnostics					667:677	endometriosis diagnostics	653:677	endometriosis diagnostics	653:677	Multiwalled carbon nanotube and magnetite nanoparticle (MWCNT-Fe3O4) was dispersed in chitosan (CS) to fabricate a bio-nanocomposite to immobilize very monoclonal specific antibody (via cross-linking using glutaraldehyde) for selective electrochemical immuno-sensing of carbohydrate antigen 19-9 (CA19-9), a potential biomarker for endometriosis diagnostics.
33524653	1	13	theme	diagnostics	296:306	arg1	application					308:318	endometriosis diagnostics application	282:318	endometriosis diagnostics application	282:318	In this research, for the first time, a bio-nanocomposites based highly sensitive and label-free electrochemical immunosensor is reported with the aim of endometriosis diagnostics application.
33524653	2	14	theme	carbohydrate	591:602	arg1	CA19-9					618:623	CA19-9	618:623	CA19-9	618:623	Multiwalled carbon nanotube and magnetite nanoparticle (MWCNT-Fe3O4) was dispersed in chitosan (CS) to fabricate a bio-nanocomposite to immobilize very monoclonal specific antibody (via cross-linking using glutaraldehyde) for selective electrochemical immuno-sensing of carbohydrate antigen 19-9 (CA19-9), a potential biomarker for endometriosis diagnostics.
33524653	2	14	theme	carbohydrate	591:602	arg1	19-9					612:615	carbohydrate antigen 19-9	591:615	carbohydrate antigen 19-9 (CA19-9)	591:624	Multiwalled carbon nanotube and magnetite nanoparticle (MWCNT-Fe3O4) was dispersed in chitosan (CS) to fabricate a bio-nanocomposite to immobilize very monoclonal specific antibody (via cross-linking using glutaraldehyde) for selective electrochemical immuno-sensing of carbohydrate antigen 19-9 (CA19-9), a potential biomarker for endometriosis diagnostics.
33524653	2	14	theme	carbohydrate	591:602	arg1	biomarker					639:647	a potential biomarker	627:647	a potential biomarker for endometriosis diagnostics	627:677	Multiwalled carbon nanotube and magnetite nanoparticle (MWCNT-Fe3O4) was dispersed in chitosan (CS) to fabricate a bio-nanocomposite to immobilize very monoclonal specific antibody (via cross-linking using glutaraldehyde) for selective electrochemical immuno-sensing of carbohydrate antigen 19-9 (CA19-9), a potential biomarker for endometriosis diagnostics.
33524653	3	15	theme	high	834:837	arg1	limit					887:891	a detection limit	875:891	a detection limit of 0.163 pg mL-1	875:908	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19-9 and exhibited a high sensitivity as (2.55 µA pg-1 cm-1), a detection limit of 0.163 pg mL-1, detection range from 1.0 pg mL-1 to 100 ng mL-1.
33524653	3	15	theme	high	834:837	arg1	cm-1					868:871	2.55 µA pg-1 cm-1	855:871	2.55 µA pg-1 cm-1	855:871	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19-9 and exhibited a high sensitivity as (2.55 µA pg-1 cm-1), a detection limit of 0.163 pg mL-1, detection range from 1.0 pg mL-1 to 100 ng mL-1.
33524653	3	15	theme	high	834:837	arg1	range					921:925	detection range	911:925	detection range	911:925	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19-9 and exhibited a high sensitivity as (2.55 µA pg-1 cm-1), a detection limit of 0.163 pg mL-1, detection range from 1.0 pg mL-1 to 100 ng mL-1.
33524653	3	15	theme	high	834:837	arg1	sensitivity					839:849	a high sensitivity	832:849	a high sensitivity as (2.55 µA pg-1 cm-1)	832:872	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19-9 and exhibited a high sensitivity as (2.55 µA pg-1 cm-1), a detection limit of 0.163 pg mL-1, detection range from 1.0 pg mL-1 to 100 ng mL-1.
33524653	4	16	theme	fabricated	964:973	arg1	immunosensor					1016:1027	Our fabricated electrochemical AbsCA19-9/CS-MWCNT-Fe3O4 immunosensor	960:1027	Our fabricated electrochemical AbsCA19-9/CS-MWCNT-Fe3O4 immunosensor	960:1027	Our fabricated electrochemical AbsCA19-9/CS-MWCNT-Fe3O4 immunosensor performed CA19-9 sensing in physiological range and at a very level which suggest it application for early-stage diagnostics, diseases monitoring, and optimization of therapy.
33524653	5	17	link	enzyme-linked	1325:1337	arg1	assay					1354:1358	enzyme-linked immune-sorbent assay	1325:1358	enzyme-linked immune-sorbent assay (ELISA)	1325:1366	To claim the clinical application, our sensor was tested using real samples and sensing performance was validated using enzyme-linked immune-sorbent assay (ELISA).
33524653	5	17	link	enzyme-linked	1325:1337	arg1	ELISA					1361:1365	ELISA	1361:1365	ELISA	1361:1365	To claim the clinical application, our sensor was tested using real samples and sensing performance was validated using enzyme-linked immune-sorbent assay (ELISA).
33524653	2	18	theme	carbon	333:338	arg1	nanotube					340:347	Multiwalled carbon nanotube	321:347	Multiwalled carbon nanotube	321:347	Multiwalled carbon nanotube and magnetite nanoparticle (MWCNT-Fe3O4) was dispersed in chitosan (CS) to fabricate a bio-nanocomposite to immobilize very monoclonal specific antibody (via cross-linking using glutaraldehyde) for selective electrochemical immuno-sensing of carbohydrate antigen 19-9 (CA19-9), a potential biomarker for endometriosis diagnostics.
33524653	1	19	theme	application	308:318	arg1	aim					275:277	the aim	271:277	the aim of endometriosis diagnostics application	271:318	In this research, for the first time, a bio-nanocomposites based highly sensitive and label-free electrochemical immunosensor is reported with the aim of endometriosis diagnostics application.
33524653	6	20	theme	techniques	1523:1532	arg1	immunosensor					1454:1465	AbsCA19-9/CS-MWCNT-Fe3O4 electrochemical CA19-9 immunosensor	1406:1465	AbsCA19-9/CS-MWCNT-Fe3O4 electrochemical CA19-9 immunosensor as a potential and affordable alternate of conventional techniques like ELISA	1406:1543	The results of the studies projected AbsCA19-9/CS-MWCNT-Fe3O4 electrochemical CA19-9 immunosensor as a potential and affordable alternate of conventional techniques like ELISA.
33524653	3	21	dep	cm-1	868:871	arg1	as					851:852	as	851:852	as	851:852	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19-9 and exhibited a high sensitivity as (2.55 µA pg-1 cm-1), a detection limit of 0.163 pg mL-1, detection range from 1.0 pg mL-1 to 100 ng mL-1.
33524653	6	22	theme	studies	1388:1394	arg1	results					1373:1379	The results	1369:1379	The results of the studies	1369:1394	The results of the studies projected AbsCA19-9/CS-MWCNT-Fe3O4 electrochemical CA19-9 immunosensor as a potential and affordable alternate of conventional techniques like ELISA.
33524653	2	23	theme	Multiwalled	321:331	arg1	nanotube					340:347	Multiwalled carbon nanotube	321:347	Multiwalled carbon nanotube	321:347	Multiwalled carbon nanotube and magnetite nanoparticle (MWCNT-Fe3O4) was dispersed in chitosan (CS) to fabricate a bio-nanocomposite to immobilize very monoclonal specific antibody (via cross-linking using glutaraldehyde) for selective electrochemical immuno-sensing of carbohydrate antigen 19-9 (CA19-9), a potential biomarker for endometriosis diagnostics.
33524653	1	24	theme	based	187:191	arg1	immunosensor					241:252	a bio-nanocomposites based highly sensitive and label-free electrochemical immunosensor	166:252	a bio-nanocomposites based highly sensitive and label-free electrochemical immunosensor	166:252	In this research, for the first time, a bio-nanocomposites based highly sensitive and label-free electrochemical immunosensor is reported with the aim of endometriosis diagnostics application.
33524653	4	25	theme	early-stage	1130:1140	arg1	diagnostics					1142:1152	early-stage diagnostics	1130:1152	early-stage diagnostics	1130:1152	Our fabricated electrochemical AbsCA19-9/CS-MWCNT-Fe3O4 immunosensor performed CA19-9 sensing in physiological range and at a very level which suggest it application for early-stage diagnostics, diseases monitoring, and optimization of therapy.
33524653	5	26	theme	enzyme-linked	1325:1337	arg1	assay					1354:1358	enzyme-linked immune-sorbent assay	1325:1358	enzyme-linked immune-sorbent assay (ELISA)	1325:1366	To claim the clinical application, our sensor was tested using real samples and sensing performance was validated using enzyme-linked immune-sorbent assay (ELISA).
33524653	5	26	theme	enzyme-linked	1325:1337	arg1	ELISA					1361:1365	ELISA	1361:1365	ELISA	1361:1365	To claim the clinical application, our sensor was tested using real samples and sensing performance was validated using enzyme-linked immune-sorbent assay (ELISA).
33524653	7	27	theme	targeted	1702:1709	arg1	biomarker					1711:1719	a targeted biomarker	1700:1719	a targeted biomarker	1700:1719	We believe that our fabricated sensor can be the plane of a disease's management program due to affordable, rapid, label-free, and sensitive detection of a targeted biomarker.
33524653	2	28	theme	electrochemical	557:571	arg1	immuno-sensing					573:586	selective electrochemical immuno-sensing	547:586	selective electrochemical immuno-sensing of carbohydrate antigen 19-9 (CA19-9), a potential biomarker for endometriosis diagnostics	547:677	Multiwalled carbon nanotube and magnetite nanoparticle (MWCNT-Fe3O4) was dispersed in chitosan (CS) to fabricate a bio-nanocomposite to immobilize very monoclonal specific antibody (via cross-linking using glutaraldehyde) for selective electrochemical immuno-sensing of carbohydrate antigen 19-9 (CA19-9), a potential biomarker for endometriosis diagnostics.
33524653	4	29	theme	AbsCA19-9/CS-MWCNT-Fe3O4	991:1014	arg1	immunosensor					1016:1027	Our fabricated electrochemical AbsCA19-9/CS-MWCNT-Fe3O4 immunosensor	960:1027	Our fabricated electrochemical AbsCA19-9/CS-MWCNT-Fe3O4 immunosensor	960:1027	Our fabricated electrochemical AbsCA19-9/CS-MWCNT-Fe3O4 immunosensor performed CA19-9 sensing in physiological range and at a very level which suggest it application for early-stage diagnostics, diseases monitoring, and optimization of therapy.
33524653	7	30	theme	management	1616:1625	arg1	program					1627:1633	a disease's management program	1604:1633	a disease's management program	1604:1633	We believe that our fabricated sensor can be the plane of a disease's management program due to affordable, rapid, label-free, and sensitive detection of a targeted biomarker.
33524653	3	31	from	1.0 pg mL-1	932:942	arg1	limit					887:891	a detection limit	875:891	a detection limit of 0.163 pg mL-1	875:908	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19-9 and exhibited a high sensitivity as (2.55 µA pg-1 cm-1), a detection limit of 0.163 pg mL-1, detection range from 1.0 pg mL-1 to 100 ng mL-1.
33524653	3	31	from	1.0 pg mL-1	932:942	arg1	cm-1					868:871	2.55 µA pg-1 cm-1	855:871	2.55 µA pg-1 cm-1	855:871	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19-9 and exhibited a high sensitivity as (2.55 µA pg-1 cm-1), a detection limit of 0.163 pg mL-1, detection range from 1.0 pg mL-1 to 100 ng mL-1.
33524653	3	31	from	1.0 pg mL-1	932:942	arg1	range					921:925	detection range	911:925	detection range	911:925	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19-9 and exhibited a high sensitivity as (2.55 µA pg-1 cm-1), a detection limit of 0.163 pg mL-1, detection range from 1.0 pg mL-1 to 100 ng mL-1.
33524653	3	31	from	1.0 pg mL-1	932:942	arg1	sensitivity					839:849	a high sensitivity	832:849	a high sensitivity as (2.55 µA pg-1 cm-1)	832:872	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19-9 and exhibited a high sensitivity as (2.55 µA pg-1 cm-1), a detection limit of 0.163 pg mL-1, detection range from 1.0 pg mL-1 to 100 ng mL-1.
33524653	0	32	theme	sensitive	31:39	arg1	immunosensor					72:83	highly sensitive and label-free electrochemical immunosensor	24:83	highly sensitive and label-free electrochemical immunosensor for endometriosis diagnostics application	24:125	Bio-nanocomposite based highly sensitive and label-free electrochemical immunosensor for endometriosis diagnostics application.
33524653	3	33	theme	µA	860:861	arg1	cm-1					868:871	2.55 µA pg-1 cm-1	855:871	2.55 µA pg-1 cm-1	855:871	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19-9 and exhibited a high sensitivity as (2.55 µA pg-1 cm-1), a detection limit of 0.163 pg mL-1, detection range from 1.0 pg mL-1 to 100 ng mL-1.
33524653	3	33	theme	µA	860:861	arg1	sensitivity					839:849	a high sensitivity	832:849	a high sensitivity as (2.55 µA pg-1 cm-1)	832:872	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19-9 and exhibited a high sensitivity as (2.55 µA pg-1 cm-1), a detection limit of 0.163 pg mL-1, detection range from 1.0 pg mL-1 to 100 ng mL-1.
33524653	2	34	theme	magnetite	353:361	arg1	MWCNT-Fe3O4					377:387	MWCNT-Fe3O4	377:387	MWCNT-Fe3O4	377:387	Multiwalled carbon nanotube and magnetite nanoparticle (MWCNT-Fe3O4) was dispersed in chitosan (CS) to fabricate a bio-nanocomposite to immobilize very monoclonal specific antibody (via cross-linking using glutaraldehyde) for selective electrochemical immuno-sensing of carbohydrate antigen 19-9 (CA19-9), a potential biomarker for endometriosis diagnostics.
33524653	2	34	theme	magnetite	353:361	arg1	nanoparticle					363:374	magnetite nanoparticle	353:374	magnetite nanoparticle (MWCNT-Fe3O4)	353:388	Multiwalled carbon nanotube and magnetite nanoparticle (MWCNT-Fe3O4) was dispersed in chitosan (CS) to fabricate a bio-nanocomposite to immobilize very monoclonal specific antibody (via cross-linking using glutaraldehyde) for selective electrochemical immuno-sensing of carbohydrate antigen 19-9 (CA19-9), a potential biomarker for endometriosis diagnostics.
33524653	3	35	theme	0.163 pg mL-1	896:908	arg1	limit					887:891	a detection limit	875:891	a detection limit of 0.163 pg mL-1	875:908	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19-9 and exhibited a high sensitivity as (2.55 µA pg-1 cm-1), a detection limit of 0.163 pg mL-1, detection range from 1.0 pg mL-1 to 100 ng mL-1.
33524653	3	35	theme	0.163 pg mL-1	896:908	arg1	sensitivity					839:849	a high sensitivity	832:849	a high sensitivity as (2.55 µA pg-1 cm-1)	832:872	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19-9 and exhibited a high sensitivity as (2.55 µA pg-1 cm-1), a detection limit of 0.163 pg mL-1, detection range from 1.0 pg mL-1 to 100 ng mL-1.
33524653	2	36	theme	monoclonal	473:482	arg1	antibody					493:500	very monoclonal specific antibody	468:500	very monoclonal specific antibody (via cross-linking using glutaraldehyde) for selective electrochemical immuno-sensing of carbohydrate antigen 19-9 (CA19-9), a potential biomarker for endometriosis diagnostics	468:677	Multiwalled carbon nanotube and magnetite nanoparticle (MWCNT-Fe3O4) was dispersed in chitosan (CS) to fabricate a bio-nanocomposite to immobilize very monoclonal specific antibody (via cross-linking using glutaraldehyde) for selective electrochemical immuno-sensing of carbohydrate antigen 19-9 (CA19-9), a potential biomarker for endometriosis diagnostics.
33524653	7	37	theme	biomarker	1711:1719	arg1	detection					1687:1695	affordable, rapid, label-free, and sensitive detection	1642:1695	affordable, rapid, label-free, and sensitive detection of a targeted biomarker	1642:1719	We believe that our fabricated sensor can be the plane of a disease's management program due to affordable, rapid, label-free, and sensitive detection of a targeted biomarker.
33524653	3	38	theme	carbon	767:772	arg1	GCE					785:787	GCE	785:787	GCE	785:787	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19-9 and exhibited a high sensitivity as (2.55 µA pg-1 cm-1), a detection limit of 0.163 pg mL-1, detection range from 1.0 pg mL-1 to 100 ng mL-1.
33524653	3	38	theme	carbon	767:772	arg1	electrode					774:782	glassy carbon electrode	760:782	glassy carbon electrode (GCE)	760:788	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19-9 and exhibited a high sensitivity as (2.55 µA pg-1 cm-1), a detection limit of 0.163 pg mL-1, detection range from 1.0 pg mL-1 to 100 ng mL-1.
33524653	7	39	theme	fabricated	1566:1575	arg1	sensor					1577:1582	our fabricated sensor	1562:1582	our fabricated sensor	1562:1582	We believe that our fabricated sensor can be the plane of a disease's management program due to affordable, rapid, label-free, and sensitive detection of a targeted biomarker.
33524653	7	39	theme	fabricated	1566:1575	arg1	plane					1595:1599	the plane	1591:1599	the plane of a disease's management program due to affordable, rapid, label-free, and sensitive detection of a targeted biomarker	1591:1719	We believe that our fabricated sensor can be the plane of a disease's management program due to affordable, rapid, label-free, and sensitive detection of a targeted biomarker.
33524653	1	40	theme	electrochemical	225:239	arg1	immunosensor					241:252	a bio-nanocomposites based highly sensitive and label-free electrochemical immunosensor	166:252	a bio-nanocomposites based highly sensitive and label-free electrochemical immunosensor	166:252	In this research, for the first time, a bio-nanocomposites based highly sensitive and label-free electrochemical immunosensor is reported with the aim of endometriosis diagnostics application.
33524653	0	41	theme	label-free	45:54	arg1	immunosensor					72:83	highly sensitive and label-free electrochemical immunosensor	24:83	highly sensitive and label-free electrochemical immunosensor for endometriosis diagnostics application	24:125	Bio-nanocomposite based highly sensitive and label-free electrochemical immunosensor for endometriosis diagnostics application.
33524653	7	42	theme	sensitive	1677:1685	arg1	detection					1687:1695	affordable, rapid, label-free, and sensitive detection	1642:1695	affordable, rapid, label-free, and sensitive detection of a targeted biomarker	1642:1719	We believe that our fabricated sensor can be the plane of a disease's management program due to affordable, rapid, label-free, and sensitive detection of a targeted biomarker.
33524653	4	43	theme	physiological	1057:1069	arg1	range					1071:1075	physiological range	1057:1075	physiological range	1057:1075	Our fabricated electrochemical AbsCA19-9/CS-MWCNT-Fe3O4 immunosensor performed CA19-9 sensing in physiological range and at a very level which suggest it application for early-stage diagnostics, diseases monitoring, and optimization of therapy.
33524653	5	44	theme	immune-sorbent	1339:1352	arg1	assay					1354:1358	enzyme-linked immune-sorbent assay	1325:1358	enzyme-linked immune-sorbent assay (ELISA)	1325:1366	To claim the clinical application, our sensor was tested using real samples and sensing performance was validated using enzyme-linked immune-sorbent assay (ELISA).
33524653	5	44	theme	immune-sorbent	1339:1352	arg1	ELISA					1361:1365	ELISA	1361:1365	ELISA	1361:1365	To claim the clinical application, our sensor was tested using real samples and sensing performance was validated using enzyme-linked immune-sorbent assay (ELISA).
33524653	5	45	theme	real	1268:1271	arg1	samples					1273:1279	real samples	1268:1279	real samples	1268:1279	To claim the clinical application, our sensor was tested using real samples and sensing performance was validated using enzyme-linked immune-sorbent assay (ELISA).
33524653	2	46	theme	19-9	612:615	arg1	immuno-sensing					573:586	selective electrochemical immuno-sensing	547:586	selective electrochemical immuno-sensing of carbohydrate antigen 19-9 (CA19-9), a potential biomarker for endometriosis diagnostics	547:677	Multiwalled carbon nanotube and magnetite nanoparticle (MWCNT-Fe3O4) was dispersed in chitosan (CS) to fabricate a bio-nanocomposite to immobilize very monoclonal specific antibody (via cross-linking using glutaraldehyde) for selective electrochemical immuno-sensing of carbohydrate antigen 19-9 (CA19-9), a potential biomarker for endometriosis diagnostics.
33524653	2	47	theme	specific	484:491	arg1	antibody					493:500	very monoclonal specific antibody	468:500	very monoclonal specific antibody (via cross-linking using glutaraldehyde) for selective electrochemical immuno-sensing of carbohydrate antigen 19-9 (CA19-9), a potential biomarker for endometriosis diagnostics	468:677	Multiwalled carbon nanotube and magnetite nanoparticle (MWCNT-Fe3O4) was dispersed in chitosan (CS) to fabricate a bio-nanocomposite to immobilize very monoclonal specific antibody (via cross-linking using glutaraldehyde) for selective electrochemical immuno-sensing of carbohydrate antigen 19-9 (CA19-9), a potential biomarker for endometriosis diagnostics.
33524653	6	48	theme	conventional	1510:1521	arg1	techniques					1523:1532	conventional techniques	1510:1532	conventional techniques like ELISA	1510:1543	The results of the studies projected AbsCA19-9/CS-MWCNT-Fe3O4 electrochemical CA19-9 immunosensor as a potential and affordable alternate of conventional techniques like ELISA.
33524653	3	49	theme	glassy	760:765	arg1	GCE					785:787	GCE	785:787	GCE	785:787	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19-9 and exhibited a high sensitivity as (2.55 µA pg-1 cm-1), a detection limit of 0.163 pg mL-1, detection range from 1.0 pg mL-1 to 100 ng mL-1.
33524653	3	49	theme	glassy	760:765	arg1	electrode					774:782	glassy carbon electrode	760:782	glassy carbon electrode (GCE)	760:788	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19-9 and exhibited a high sensitivity as (2.55 µA pg-1 cm-1), a detection limit of 0.163 pg mL-1, detection range from 1.0 pg mL-1 to 100 ng mL-1.
33524653	1	50	dep	based	187:191	arg1	sensitive					200:208	sensitive	200:208	sensitive	200:208	In this research, for the first time, a bio-nanocomposites based highly sensitive and label-free electrochemical immunosensor is reported with the aim of endometriosis diagnostics application.
33524653	1	50	dep	based	187:191	arg1	label-free					214:223	label-free	214:223	label-free	214:223	In this research, for the first time, a bio-nanocomposites based highly sensitive and label-free electrochemical immunosensor is reported with the aim of endometriosis diagnostics application.
33524653	0	51	theme	electrochemical	56:70	arg1	immunosensor					72:83	highly sensitive and label-free electrochemical immunosensor	24:83	highly sensitive and label-free electrochemical immunosensor for endometriosis diagnostics application	24:125	Bio-nanocomposite based highly sensitive and label-free electrochemical immunosensor for endometriosis diagnostics application.
33524653	3	52	theme	pg-1	863:866	arg1	cm-1					868:871	2.55 µA pg-1 cm-1	855:871	2.55 µA pg-1 cm-1	855:871	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19-9 and exhibited a high sensitivity as (2.55 µA pg-1 cm-1), a detection limit of 0.163 pg mL-1, detection range from 1.0 pg mL-1 to 100 ng mL-1.
33524653	3	52	theme	pg-1	863:866	arg1	sensitivity					839:849	a high sensitivity	832:849	a high sensitivity as (2.55 µA pg-1 cm-1)	832:872	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19-9 and exhibited a high sensitivity as (2.55 µA pg-1 cm-1), a detection limit of 0.163 pg mL-1, detection range from 1.0 pg mL-1 to 100 ng mL-1.
33524653	2	53	theme	potential	629:637	arg1	19-9					612:615	carbohydrate antigen 19-9	591:615	carbohydrate antigen 19-9 (CA19-9)	591:624	Multiwalled carbon nanotube and magnetite nanoparticle (MWCNT-Fe3O4) was dispersed in chitosan (CS) to fabricate a bio-nanocomposite to immobilize very monoclonal specific antibody (via cross-linking using glutaraldehyde) for selective electrochemical immuno-sensing of carbohydrate antigen 19-9 (CA19-9), a potential biomarker for endometriosis diagnostics.
33524653	2	53	theme	potential	629:637	arg1	biomarker					639:647	a potential biomarker	627:647	a potential biomarker for endometriosis diagnostics	627:677	Multiwalled carbon nanotube and magnetite nanoparticle (MWCNT-Fe3O4) was dispersed in chitosan (CS) to fabricate a bio-nanocomposite to immobilize very monoclonal specific antibody (via cross-linking using glutaraldehyde) for selective electrochemical immuno-sensing of carbohydrate antigen 19-9 (CA19-9), a potential biomarker for endometriosis diagnostics.
33524653	4	54	theme	electrochemical	975:989	arg1	immunosensor					1016:1027	Our fabricated electrochemical AbsCA19-9/CS-MWCNT-Fe3O4 immunosensor	960:1027	Our fabricated electrochemical AbsCA19-9/CS-MWCNT-Fe3O4 immunosensor	960:1027	Our fabricated electrochemical AbsCA19-9/CS-MWCNT-Fe3O4 immunosensor performed CA19-9 sensing in physiological range and at a very level which suggest it application for early-stage diagnostics, diseases monitoring, and optimization of therapy.
33524653	5	55	theme	sensing	1285:1291	arg1	performance					1293:1303	sensing performance	1285:1303	sensing performance	1285:1303	To claim the clinical application, our sensor was tested using real samples and sensing performance was validated using enzyme-linked immune-sorbent assay (ELISA).
33524653	4	56	theme	CA19-9	1039:1044	arg1	sensing					1046:1052	CA19-9 sensing	1039:1052	CA19-9 sensing	1039:1052	Our fabricated electrochemical AbsCA19-9/CS-MWCNT-Fe3O4 immunosensor performed CA19-9 sensing in physiological range and at a very level which suggest it application for early-stage diagnostics, diseases monitoring, and optimization of therapy.
33524653	7	57	theme	label-free	1661:1670	arg1	detection					1687:1695	affordable, rapid, label-free, and sensitive detection	1642:1695	affordable, rapid, label-free, and sensitive detection of a targeted biomarker	1642:1719	We believe that our fabricated sensor can be the plane of a disease's management program due to affordable, rapid, label-free, and sensitive detection of a targeted biomarker.
33524653	1	58	theme	first	154:158	arg1	time					160:163	the first time	150:163	the first time	150:163	In this research, for the first time, a bio-nanocomposites based highly sensitive and label-free electrochemical immunosensor is reported with the aim of endometriosis diagnostics application.
33524653	0	59	theme	endometriosis	89:101	arg1	application					115:125	endometriosis diagnostics application	89:125	endometriosis diagnostics application	89:125	Bio-nanocomposite based highly sensitive and label-free electrochemical immunosensor for endometriosis diagnostics application.
33524653	7	60	theme	rapid	1654:1658	arg1	detection					1687:1695	affordable, rapid, label-free, and sensitive detection	1642:1695	affordable, rapid, label-free, and sensitive detection of a targeted biomarker	1642:1719	We believe that our fabricated sensor can be the plane of a disease's management program due to affordable, rapid, label-free, and sensitive detection of a targeted biomarker.
33524653	3	61	theme	Well-characterized	680:697	arg1	immune-electrode					729:744	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode	680:744	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE)	680:788	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19-9 and exhibited a high sensitivity as (2.55 µA pg-1 cm-1), a detection limit of 0.163 pg mL-1, detection range from 1.0 pg mL-1 to 100 ng mL-1.
33524653	2	62	theme	selective	547:555	arg1	immuno-sensing					573:586	selective electrochemical immuno-sensing	547:586	selective electrochemical immuno-sensing of carbohydrate antigen 19-9 (CA19-9), a potential biomarker for endometriosis diagnostics	547:677	Multiwalled carbon nanotube and magnetite nanoparticle (MWCNT-Fe3O4) was dispersed in chitosan (CS) to fabricate a bio-nanocomposite to immobilize very monoclonal specific antibody (via cross-linking using glutaraldehyde) for selective electrochemical immuno-sensing of carbohydrate antigen 19-9 (CA19-9), a potential biomarker for endometriosis diagnostics.
33524653	3	63	theme	Anti-AbsCA19-9/CS-MWCNT-Fe3O4	699:727	arg1	immune-electrode					729:744	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode	680:744	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE)	680:788	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19-9 and exhibited a high sensitivity as (2.55 µA pg-1 cm-1), a detection limit of 0.163 pg mL-1, detection range from 1.0 pg mL-1 to 100 ng mL-1.
33524653	6	64	theme	AbsCA19-9/CS-MWCNT-Fe3O4	1406:1429	arg1	immunosensor					1454:1465	AbsCA19-9/CS-MWCNT-Fe3O4 electrochemical CA19-9 immunosensor	1406:1465	AbsCA19-9/CS-MWCNT-Fe3O4 electrochemical CA19-9 immunosensor as a potential and affordable alternate of conventional techniques like ELISA	1406:1543	The results of the studies projected AbsCA19-9/CS-MWCNT-Fe3O4 electrochemical CA19-9 immunosensor as a potential and affordable alternate of conventional techniques like ELISA.
33524653	3	65	theme	detection	877:885	arg1	limit					887:891	a detection limit	875:891	a detection limit of 0.163 pg mL-1	875:908	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19-9 and exhibited a high sensitivity as (2.55 µA pg-1 cm-1), a detection limit of 0.163 pg mL-1, detection range from 1.0 pg mL-1 to 100 ng mL-1.
33524653	3	65	theme	detection	877:885	arg1	sensitivity					839:849	a high sensitivity	832:849	a high sensitivity as (2.55 µA pg-1 cm-1)	832:872	Well-characterized Anti-AbsCA19-9/CS-MWCNT-Fe3O4 immune-electrode fabricated on glassy carbon electrode (GCE) successfully detect CA 19-9 and exhibited a high sensitivity as (2.55 µA pg-1 cm-1), a detection limit of 0.163 pg mL-1, detection range from 1.0 pg mL-1 to 100 ng mL-1.
32456103	9	0	theme	such	1231:1234	arg1	laminates					1247:1255	such whey-based laminates	1231:1255	such whey-based laminates	1231:1255	Furthermore, such whey-based laminates still show excellent barrier properties, good antimicrobial activity and a remarkable antioxidative activity.
32456103	4	1	theme	layer	702:706	arg1	deposition					713:722	layer wise deposition	702:722	layer wise deposition	702:722	Additionally, a whey-based laminate was functionalised: i) chitosan macromolecular solution was applied as first layer and ii) cinnamon or rosemary extracts encapsulated in CSNPs were applied as upper layer (layer wise deposition).
32456103	4	1	theme	layer	702:706	arg1	layer					695:699	upper layer	689:699	upper layer (layer wise deposition)	689:723	Additionally, a whey-based laminate was functionalised: i) chitosan macromolecular solution was applied as first layer and ii) cinnamon or rosemary extracts encapsulated in CSNPs were applied as upper layer (layer wise deposition).
32456103	5	2	dep	whey-based	746:755	arg1	laminate					757:764	laminate	757:764	laminate	757:764	Such functionalised whey-based laminate was physicochemically characterized in terms of elemental surface composition, wettability, morphology and oxygen permeability.
32456103	4	3	theme	first	601:605	arg1	layer					607:611	first layer	601:611	first layer	601:611	Additionally, a whey-based laminate was functionalised: i) chitosan macromolecular solution was applied as first layer and ii) cinnamon or rosemary extracts encapsulated in CSNPs were applied as upper layer (layer wise deposition).
32456103	4	3	theme	first	601:605	arg1	solution					577:584	i) chitosan macromolecular solution	550:584	i) chitosan macromolecular solution	550:584	Additionally, a whey-based laminate was functionalised: i) chitosan macromolecular solution was applied as first layer and ii) cinnamon or rosemary extracts encapsulated in CSNPs were applied as upper layer (layer wise deposition).
32456103	10	4	theme	structured	1518:1527	arg1	films					1529:1533	such structured films	1513:1533	such structured films	1513:1533	In addition to the improved biodegradability, this type of lamination could also have a positive effect on the shelf-life of products packaged in such structured films.
32456103	9	5	theme	whey-based	1236:1245	arg1	laminates					1247:1255	such whey-based laminates	1231:1255	such whey-based laminates	1231:1255	Furthermore, such whey-based laminates still show excellent barrier properties, good antimicrobial activity and a remarkable antioxidative activity.
32456103	5	6	theme	elemental	814:822	arg1	composition					832:842	elemental surface composition	814:842	elemental surface composition	814:842	Such functionalised whey-based laminate was physicochemically characterized in terms of elemental surface composition, wettability, morphology and oxygen permeability.
32456103	10	7	theme	products	1492:1499	arg1	shelf-life					1478:1487	the shelf-life	1474:1487	the shelf-life of products packaged in such structured films	1474:1533	In addition to the improved biodegradability, this type of lamination could also have a positive effect on the shelf-life of products packaged in such structured films.
32456103	10	8	theme	such	1513:1516	arg1	films					1529:1533	such structured films	1513:1533	such structured films	1513:1533	In addition to the improved biodegradability, this type of lamination could also have a positive effect on the shelf-life of products packaged in such structured films.
32456103	8	9	theme	above-mentioned	1162:1176	arg1	strategy					1178:1185	the above-mentioned strategy	1158:1185	the above-mentioned strategy	1158:1185	It could be shown that after functionalization of the films with the above-mentioned strategy, the wettability was improved.
32456103	1	10	used	used	188:191	arg2	extracts					174:181	antimicrobial polysaccharide chitosan and natural extracts	124:181	antimicrobial polysaccharide chitosan and natural extracts	124:181	In this research, antimicrobial polysaccharide chitosan and natural extracts were used as surface coating of a plastic laminate with an integrated whey layer on the inside.
32456103	1	10	used	used	188:191	arg2	coating					204:210	surface coating	196:210	surface coating of a plastic laminate with an integrated whey layer on the inside	196:276	In this research, antimicrobial polysaccharide chitosan and natural extracts were used as surface coating of a plastic laminate with an integrated whey layer on the inside.
32456103	2	11	theme	laminates	354:362	arg1	concept					333:339	the biodegradable and active concept	304:339	the biodegradable and active concept of packaging laminates	304:362	The aim was to establish the biodegradable and active concept of packaging laminates.
32456103	10	12	theme	positive	1455:1462	arg1	effect					1464:1469	a positive effect	1453:1469	a positive effect	1453:1469	In addition to the improved biodegradability, this type of lamination could also have a positive effect on the shelf-life of products packaged in such structured films.
32456103	1	13	theme	surface	196:202	arg1	coating					204:210	surface coating	196:210	surface coating of a plastic laminate with an integrated whey layer on the inside	196:276	In this research, antimicrobial polysaccharide chitosan and natural extracts were used as surface coating of a plastic laminate with an integrated whey layer on the inside.
32456103	1	13	theme	surface	196:202	arg1	extracts					174:181	antimicrobial polysaccharide chitosan and natural extracts	124:181	antimicrobial polysaccharide chitosan and natural extracts	124:181	In this research, antimicrobial polysaccharide chitosan and natural extracts were used as surface coating of a plastic laminate with an integrated whey layer on the inside.
32456103	9	14	theme	excellent	1268:1276	arg1	properties					1286:1295	excellent barrier properties	1268:1295	excellent barrier properties	1268:1295	Furthermore, such whey-based laminates still show excellent barrier properties, good antimicrobial activity and a remarkable antioxidative activity.
32456103	10	15	contain	have	1448:1451	arg1	type					1418:1421	this type	1413:1421	this type of lamination	1413:1435	In addition to the improved biodegradability, this type of lamination could also have a positive effect on the shelf-life of products packaged in such structured films.
32456103	10	15	contain	have	1448:1451	arg2	effect					1464:1469	a positive effect	1453:1469	a positive effect	1453:1469	In addition to the improved biodegradability, this type of lamination could also have a positive effect on the shelf-life of products packaged in such structured films.
32456103	5	16	theme	composition	832:842	arg1	terms					805:809	terms	805:809	terms of elemental surface composition, wettability, morphology and oxygen permeability	805:891	Such functionalised whey-based laminate was physicochemically characterized in terms of elemental surface composition, wettability, morphology and oxygen permeability.
32456103	9	17	theme	barrier	1278:1284	arg1	properties					1286:1295	excellent barrier properties	1268:1295	excellent barrier properties	1268:1295	Furthermore, such whey-based laminates still show excellent barrier properties, good antimicrobial activity and a remarkable antioxidative activity.
32456103	2	18	theme	active	326:331	arg1	concept					333:339	the biodegradable and active concept	304:339	the biodegradable and active concept of packaging laminates	304:362	The aim was to establish the biodegradable and active concept of packaging laminates.
32456103	8	19	with	functionalization	1122:1138	arg1	strategy					1178:1185	the above-mentioned strategy	1158:1185	the above-mentioned strategy	1158:1185	It could be shown that after functionalization of the films with the above-mentioned strategy, the wettability was improved.
32456103	9	20	theme	antimicrobial	1303:1315	arg1	activity					1317:1324	good antimicrobial activity	1298:1324	good antimicrobial activity	1298:1324	Furthermore, such whey-based laminates still show excellent barrier properties, good antimicrobial activity and a remarkable antioxidative activity.
32456103	0	21	theme	Whey-Based	29:38	arg1	Development					0:10	Development	0:10	Development of Biodegradable Whey-Based	0:38	Development of Biodegradable Whey-Based Laminate Functionalised by Chitosan-Natural Extract Formulations.
32456103	4	22	theme	macromolecular	562:575	arg1	layer					607:611	first layer	601:611	first layer	601:611	Additionally, a whey-based laminate was functionalised: i) chitosan macromolecular solution was applied as first layer and ii) cinnamon or rosemary extracts encapsulated in CSNPs were applied as upper layer (layer wise deposition).
32456103	4	22	theme	macromolecular	562:575	arg1	solution					577:584	i) chitosan macromolecular solution	550:584	i) chitosan macromolecular solution	550:584	Additionally, a whey-based laminate was functionalised: i) chitosan macromolecular solution was applied as first layer and ii) cinnamon or rosemary extracts encapsulated in CSNPs were applied as upper layer (layer wise deposition).
32456103	4	22	theme	macromolecular	562:575	arg1	cinnamon					621:628	ii) cinnamon	617:628	ii) cinnamon	617:628	Additionally, a whey-based laminate was functionalised: i) chitosan macromolecular solution was applied as first layer and ii) cinnamon or rosemary extracts encapsulated in CSNPs were applied as upper layer (layer wise deposition).
32456103	2	23	theme	biodegradable	308:320	arg1	concept					333:339	the biodegradable and active concept	304:339	the biodegradable and active concept of packaging laminates	304:362	The aim was to establish the biodegradable and active concept of packaging laminates.
32456103	1	24	theme	plastic	217:223	arg1	coating					204:210	surface coating	196:210	surface coating of a plastic laminate with an integrated whey layer on the inside	196:276	In this research, antimicrobial polysaccharide chitosan and natural extracts were used as surface coating of a plastic laminate with an integrated whey layer on the inside.
32456103	1	24	theme	plastic	217:223	arg1	extracts					174:181	antimicrobial polysaccharide chitosan and natural extracts	124:181	antimicrobial polysaccharide chitosan and natural extracts	124:181	In this research, antimicrobial polysaccharide chitosan and natural extracts were used as surface coating of a plastic laminate with an integrated whey layer on the inside.
32456103	3	25	theme	chitosan	383:390	arg1	nanoparticles					392:404	chitosan nanoparticles	383:404	chitosan nanoparticles (CSNPs) with embedded rosemary or cinnamon extracts	383:456	For this purpose, chitosan nanoparticles (CSNPs) with embedded rosemary or cinnamon extracts were synthesised and characterised.
32456103	3	25	theme	chitosan	383:390	arg1	CSNPs					407:411	CSNPs	407:411	CSNPs	407:411	For this purpose, chitosan nanoparticles (CSNPs) with embedded rosemary or cinnamon extracts were synthesised and characterised.
32456103	9	26	theme	good	1298:1301	arg1	activity					1317:1324	good antimicrobial activity	1298:1324	good antimicrobial activity	1298:1324	Furthermore, such whey-based laminates still show excellent barrier properties, good antimicrobial activity and a remarkable antioxidative activity.
32456103	1	27	theme	antimicrobial	124:136	arg1	chitosan					153:160	antimicrobial polysaccharide chitosan	124:160	antimicrobial polysaccharide chitosan	124:160	In this research, antimicrobial polysaccharide chitosan and natural extracts were used as surface coating of a plastic laminate with an integrated whey layer on the inside.
32456103	10	28	theme	improved	1386:1393	arg1	biodegradability					1395:1410	the improved biodegradability	1382:1410	the improved biodegradability	1382:1410	In addition to the improved biodegradability, this type of lamination could also have a positive effect on the shelf-life of products packaged in such structured films.
32456103	4	29	theme	rosemary	633:640	arg1	extracts					642:649	rosemary extracts	633:649	rosemary extracts encapsulated in CSNPs	633:671	Additionally, a whey-based laminate was functionalised: i) chitosan macromolecular solution was applied as first layer and ii) cinnamon or rosemary extracts encapsulated in CSNPs were applied as upper layer (layer wise deposition).
32456103	4	29	theme	rosemary	633:640	arg1	layer					695:699	upper layer	689:699	upper layer (layer wise deposition)	689:723	Additionally, a whey-based laminate was functionalised: i) chitosan macromolecular solution was applied as first layer and ii) cinnamon or rosemary extracts encapsulated in CSNPs were applied as upper layer (layer wise deposition).
32456103	2	30	theme	packaging	344:352	arg1	laminates					354:362	packaging laminates	344:362	packaging laminates	344:362	The aim was to establish the biodegradable and active concept of packaging laminates.
32456103	6	31	theme	antimicrobial	898:910	arg1	activity					912:919	The antimicrobial activity	894:919	The antimicrobial activity	894:919	The antimicrobial activity was tested against Staphylococcus aureus, Escherichia coli, Aspergillus flavus and Penicillium verrucosum.
32456103	5	32	theme	oxygen	873:878	arg1	permeability					880:891	oxygen permeability	873:891	oxygen permeability	873:891	Such functionalised whey-based laminate was physicochemically characterized in terms of elemental surface composition, wettability, morphology and oxygen permeability.
32456103	3	33	theme	cinnamon	440:447	arg1	extracts					449:456	cinnamon extracts	440:456	cinnamon extracts	440:456	For this purpose, chitosan nanoparticles (CSNPs) with embedded rosemary or cinnamon extracts were synthesised and characterised.
32456103	3	34	theme	embedded	419:426	arg1	rosemary					428:435	embedded rosemary	419:435	embedded rosemary	419:435	For this purpose, chitosan nanoparticles (CSNPs) with embedded rosemary or cinnamon extracts were synthesised and characterised.
32456103	5	35	theme	wettability	845:855	arg1	terms					805:809	terms	805:809	terms of elemental surface composition, wettability, morphology and oxygen permeability	805:891	Such functionalised whey-based laminate was physicochemically characterized in terms of elemental surface composition, wettability, morphology and oxygen permeability.
32456103	7	36	theme	ABTS	1081:1084	arg1	assay					1086:1090	the ABTS assay	1077:1090	the ABTS assay	1077:1090	The antioxidant properties were determined using the ABTS assay.
32456103	5	37	theme	permeability	880:891	arg1	terms					805:809	terms	805:809	terms of elemental surface composition, wettability, morphology and oxygen permeability	805:891	Such functionalised whey-based laminate was physicochemically characterized in terms of elemental surface composition, wettability, morphology and oxygen permeability.
32456103	0	38	theme	Chitosan-Natural	67:82	arg1	Formulations					92:103	Chitosan-Natural Extract Formulations	67:103	Chitosan-Natural Extract Formulations	67:103	Development of Biodegradable Whey-Based Laminate Functionalised by Chitosan-Natural Extract Formulations.
32456103	1	39	from	layer	258:262	arg1	inside					271:276	the inside	267:276	the inside	267:276	In this research, antimicrobial polysaccharide chitosan and natural extracts were used as surface coating of a plastic laminate with an integrated whey layer on the inside.
32456103	1	40	theme	polysaccharide	138:151	arg1	chitosan					153:160	antimicrobial polysaccharide chitosan	124:160	antimicrobial polysaccharide chitosan	124:160	In this research, antimicrobial polysaccharide chitosan and natural extracts were used as surface coating of a plastic laminate with an integrated whey layer on the inside.
32456103	5	41	theme	surface	824:830	arg1	composition					832:842	elemental surface composition	814:842	elemental surface composition	814:842	Such functionalised whey-based laminate was physicochemically characterized in terms of elemental surface composition, wettability, morphology and oxygen permeability.
32456103	4	42	theme	chitosan	553:560	arg1	layer					607:611	first layer	601:611	first layer	601:611	Additionally, a whey-based laminate was functionalised: i) chitosan macromolecular solution was applied as first layer and ii) cinnamon or rosemary extracts encapsulated in CSNPs were applied as upper layer (layer wise deposition).
32456103	4	42	theme	chitosan	553:560	arg1	solution					577:584	i) chitosan macromolecular solution	550:584	i) chitosan macromolecular solution	550:584	Additionally, a whey-based laminate was functionalised: i) chitosan macromolecular solution was applied as first layer and ii) cinnamon or rosemary extracts encapsulated in CSNPs were applied as upper layer (layer wise deposition).
32456103	4	42	theme	chitosan	553:560	arg1	cinnamon					621:628	ii) cinnamon	617:628	ii) cinnamon	617:628	Additionally, a whey-based laminate was functionalised: i) chitosan macromolecular solution was applied as first layer and ii) cinnamon or rosemary extracts encapsulated in CSNPs were applied as upper layer (layer wise deposition).
32456103	0	43	dep	Laminate	40:47	arg1	Functionalised					49:62	Functionalised	49:62	Laminate Functionalised by Chitosan-Natural Extract Formulations	40:103	Development of Biodegradable Whey-Based Laminate Functionalised by Chitosan-Natural Extract Formulations.
32456103	1	44	theme	chitosan	153:160	arg1	coating					204:210	surface coating	196:210	surface coating of a plastic laminate with an integrated whey layer on the inside	196:276	In this research, antimicrobial polysaccharide chitosan and natural extracts were used as surface coating of a plastic laminate with an integrated whey layer on the inside.
32456103	1	44	theme	chitosan	153:160	arg1	extracts					174:181	antimicrobial polysaccharide chitosan and natural extracts	124:181	antimicrobial polysaccharide chitosan and natural extracts	124:181	In this research, antimicrobial polysaccharide chitosan and natural extracts were used as surface coating of a plastic laminate with an integrated whey layer on the inside.
32456103	4	45	dep	whey-based	510:519	arg1	laminate					521:528	laminate	521:528	laminate	521:528	Additionally, a whey-based laminate was functionalised: i) chitosan macromolecular solution was applied as first layer and ii) cinnamon or rosemary extracts encapsulated in CSNPs were applied as upper layer (layer wise deposition).
32456103	4	46	dep	cinnamon	621:628	arg1	ii					617:618	ii	617:618	ii	617:618	Additionally, a whey-based laminate was functionalised: i) chitosan macromolecular solution was applied as first layer and ii) cinnamon or rosemary extracts encapsulated in CSNPs were applied as upper layer (layer wise deposition).
32456103	10	47	dep	biodegradability	1395:1410	arg1	addition					1370:1377	addition	1370:1377	addition	1370:1377	In addition to the improved biodegradability, this type of lamination could also have a positive effect on the shelf-life of products packaged in such structured films.
32456103	3	48	with	nanoparticles	392:404	arg1	rosemary					428:435	embedded rosemary	419:435	embedded rosemary	419:435	For this purpose, chitosan nanoparticles (CSNPs) with embedded rosemary or cinnamon extracts were synthesised and characterised.
32456103	3	48	with	nanoparticles	392:404	arg1	extracts					449:456	cinnamon extracts	440:456	cinnamon extracts	440:456	For this purpose, chitosan nanoparticles (CSNPs) with embedded rosemary or cinnamon extracts were synthesised and characterised.
32456103	1	49	theme	integrated	242:251	arg1	layer					258:262	an integrated whey layer	239:262	an integrated whey layer on the inside	239:276	In this research, antimicrobial polysaccharide chitosan and natural extracts were used as surface coating of a plastic laminate with an integrated whey layer on the inside.
32456103	9	50	theme	antioxidative	1343:1355	arg1	activity					1357:1364	a remarkable antioxidative activity	1330:1364	a remarkable antioxidative activity	1330:1364	Furthermore, such whey-based laminates still show excellent barrier properties, good antimicrobial activity and a remarkable antioxidative activity.
32456103	10	51	theme	lamination	1426:1435	arg1	type					1418:1421	this type	1413:1421	this type of lamination	1413:1435	In addition to the improved biodegradability, this type of lamination could also have a positive effect on the shelf-life of products packaged in such structured films.
32456103	1	52	theme	natural	166:172	arg1	coating					204:210	surface coating	196:210	surface coating of a plastic laminate with an integrated whey layer on the inside	196:276	In this research, antimicrobial polysaccharide chitosan and natural extracts were used as surface coating of a plastic laminate with an integrated whey layer on the inside.
32456103	1	52	theme	natural	166:172	arg1	extracts					174:181	antimicrobial polysaccharide chitosan and natural extracts	124:181	antimicrobial polysaccharide chitosan and natural extracts	124:181	In this research, antimicrobial polysaccharide chitosan and natural extracts were used as surface coating of a plastic laminate with an integrated whey layer on the inside.
32456103	1	53	theme	whey	253:256	arg1	layer					258:262	an integrated whey layer	239:262	an integrated whey layer on the inside	239:276	In this research, antimicrobial polysaccharide chitosan and natural extracts were used as surface coating of a plastic laminate with an integrated whey layer on the inside.
32456103	1	54	dep	plastic	217:223	arg1	laminate					225:232	laminate	225:232	laminate with an integrated whey layer on the inside	225:276	In this research, antimicrobial polysaccharide chitosan and natural extracts were used as surface coating of a plastic laminate with an integrated whey layer on the inside.
32456103	5	55	theme	morphology	858:867	arg1	terms					805:809	terms	805:809	terms of elemental surface composition, wettability, morphology and oxygen permeability	805:891	Such functionalised whey-based laminate was physicochemically characterized in terms of elemental surface composition, wettability, morphology and oxygen permeability.
32456103	9	56	theme	remarkable	1332:1341	arg1	activity					1357:1364	a remarkable antioxidative activity	1330:1364	a remarkable antioxidative activity	1330:1364	Furthermore, such whey-based laminates still show excellent barrier properties, good antimicrobial activity and a remarkable antioxidative activity.
32456103	0	57	theme	Extract	84:90	arg1	Formulations					92:103	Chitosan-Natural Extract Formulations	67:103	Chitosan-Natural Extract Formulations	67:103	Development of Biodegradable Whey-Based Laminate Functionalised by Chitosan-Natural Extract Formulations.
32456103	0	58	theme	Biodegradable	15:27	arg1	Whey-Based					29:38	Biodegradable Whey-Based	15:38	Biodegradable Whey-Based	15:38	Development of Biodegradable Whey-Based Laminate Functionalised by Chitosan-Natural Extract Formulations.
32456103	7	59	theme	antioxidant	1032:1042	arg1	properties					1044:1053	The antioxidant properties	1028:1053	The antioxidant properties	1028:1053	The antioxidant properties were determined using the ABTS assay.
32456103	4	60	theme	upper	689:693	arg1	deposition					713:722	layer wise deposition	702:722	layer wise deposition	702:722	Additionally, a whey-based laminate was functionalised: i) chitosan macromolecular solution was applied as first layer and ii) cinnamon or rosemary extracts encapsulated in CSNPs were applied as upper layer (layer wise deposition).
32456103	4	60	theme	upper	689:693	arg1	layer					695:699	upper layer	689:699	upper layer (layer wise deposition)	689:723	Additionally, a whey-based laminate was functionalised: i) chitosan macromolecular solution was applied as first layer and ii) cinnamon or rosemary extracts encapsulated in CSNPs were applied as upper layer (layer wise deposition).
32456103	4	60	theme	upper	689:693	arg1	extracts					642:649	rosemary extracts	633:649	rosemary extracts encapsulated in CSNPs	633:671	Additionally, a whey-based laminate was functionalised: i) chitosan macromolecular solution was applied as first layer and ii) cinnamon or rosemary extracts encapsulated in CSNPs were applied as upper layer (layer wise deposition).
32456103	8	61	theme	films	1147:1151	arg1	functionalization					1122:1138	functionalization	1122:1138	functionalization of the films with the above-mentioned strategy	1122:1185	It could be shown that after functionalization of the films with the above-mentioned strategy, the wettability was improved.
32456103	4	62	theme	wise	708:711	arg1	deposition					713:722	layer wise deposition	702:722	layer wise deposition	702:722	Additionally, a whey-based laminate was functionalised: i) chitosan macromolecular solution was applied as first layer and ii) cinnamon or rosemary extracts encapsulated in CSNPs were applied as upper layer (layer wise deposition).
32456103	4	62	theme	wise	708:711	arg1	layer					695:699	upper layer	689:699	upper layer (layer wise deposition)	689:723	Additionally, a whey-based laminate was functionalised: i) chitosan macromolecular solution was applied as first layer and ii) cinnamon or rosemary extracts encapsulated in CSNPs were applied as upper layer (layer wise deposition).
33766383	2	0	contain	possessing	398:407	arg1	compound					389:396	a plant-derived active compound	366:396	a plant-derived active compound possessing several bioactivities	366:429	Chitosan-silica hybrid nanocomposite scaffolds loaded with mangiferin, a plant-derived active compound possessing several bioactivities, were fabricated using the sol-gel synthesis and the freeze-drying processes.
33766383	2	0	contain	possessing	398:407	arg1	mangiferin					354:363	mangiferin	354:363	mangiferin	354:363	Chitosan-silica hybrid nanocomposite scaffolds loaded with mangiferin, a plant-derived active compound possessing several bioactivities, were fabricated using the sol-gel synthesis and the freeze-drying processes.
33766383	2	0	contain	possessing	398:407	arg2	bioactivities					417:429	several bioactivities	409:429	several bioactivities	409:429	Chitosan-silica hybrid nanocomposite scaffolds loaded with mangiferin, a plant-derived active compound possessing several bioactivities, were fabricated using the sol-gel synthesis and the freeze-drying processes.
33766383	1	1	theme	co-network	195:204	arg1	structure					164:172	molecular structure	154:172	molecular structure of organic-inorganic co-network	154:204	Development of hybrid materials with molecular structure of organic-inorganic co-network is a promising method to enhance the stability and mechanical properties of biopolymers.
33766383	0	2	theme	mangiferin	15:24	arg1	scaffolds					56:64	mangiferin loaded chitosan-silica hybrid scaffolds	15:64	mangiferin loaded chitosan-silica hybrid scaffolds	15:64	Development of mangiferin loaded chitosan-silica hybrid scaffolds: Physicochemical and bioactivity characterization.
33766383	5	3	theme	nanocomposite	1059:1071	arg1	scaffolds					1073:1081	the hybrid nanocomposite scaffolds	1048:1081	the hybrid nanocomposite scaffolds in guided bone regeneration application	1048:1121	Investigation on the biomineralization and cell viability indicated that the inclusion of bioactive mangiferin further promote potential use of the hybrid nanocomposite scaffolds in guided bone regeneration application.
33766383	5	4	from	Investigation	904:916	arg1	viability					952:960	the biomineralization and cell viability	921:960	viability	952:960	Investigation on the biomineralization and cell viability indicated that the inclusion of bioactive mangiferin further promote potential use of the hybrid nanocomposite scaffolds in guided bone regeneration application.
33766383	3	5	theme	ZnO	732:734	arg1	nanoparticles					736:748	ZnO nanoparticles	732:748	ZnO nanoparticles	732:748	Investigation on the physicochemical and mechanical properties of the fabricated scaffolds showed that their properties can be improved and tailored by the formation of 3-dimensional crosslinked network and the addition of ZnO nanoparticles.
33766383	4	6	theme	engineering	879:889	arg1	application					891:901	bone tissue engineering application	867:901	bone tissue engineering application	867:901	The scaffolds possessed porosity, fluid uptake, morphology, thermal properties and mechanical strength suitable for bone tissue engineering application.
33766383	0	7	dep	Physicochemical	67:81	arg1	characterization					99:114	characterization	99:114	characterization	99:114	Development of mangiferin loaded chitosan-silica hybrid scaffolds: Physicochemical and bioactivity characterization.
33766383	4	8	theme	suitable	854:861	arg1	porosity					775:782	porosity	775:782	porosity	775:782	The scaffolds possessed porosity, fluid uptake, morphology, thermal properties and mechanical strength suitable for bone tissue engineering application.
33766383	4	9	theme	tissue	872:877	arg1	application					891:901	bone tissue engineering application	867:901	bone tissue engineering application	867:901	The scaffolds possessed porosity, fluid uptake, morphology, thermal properties and mechanical strength suitable for bone tissue engineering application.
33766383	3	10	theme	scaffolds	590:598	arg1	properties					561:570	the physicochemical and mechanical properties	526:570	the physicochemical and mechanical properties of the fabricated scaffolds	526:598	Investigation on the physicochemical and mechanical properties of the fabricated scaffolds showed that their properties can be improved and tailored by the formation of 3-dimensional crosslinked network and the addition of ZnO nanoparticles.
33766383	5	11	from	use	1041:1043	arg1	application					1111:1121	guided bone regeneration application	1086:1121	guided bone regeneration application	1086:1121	Investigation on the biomineralization and cell viability indicated that the inclusion of bioactive mangiferin further promote potential use of the hybrid nanocomposite scaffolds in guided bone regeneration application.
33766383	4	12	theme	fluid	785:789	arg1	uptake					791:796	fluid uptake	785:796	fluid uptake	785:796	The scaffolds possessed porosity, fluid uptake, morphology, thermal properties and mechanical strength suitable for bone tissue engineering application.
33766383	5	13	theme	bioactive	994:1002	arg1	mangiferin					1004:1013	bioactive mangiferin	994:1013	bioactive mangiferin	994:1013	Investigation on the biomineralization and cell viability indicated that the inclusion of bioactive mangiferin further promote potential use of the hybrid nanocomposite scaffolds in guided bone regeneration application.
33766383	5	14	theme	biomineralization	925:941	arg1	viability					952:960	the biomineralization and cell viability	921:960	viability	952:960	Investigation on the biomineralization and cell viability indicated that the inclusion of bioactive mangiferin further promote potential use of the hybrid nanocomposite scaffolds in guided bone regeneration application.
33766383	5	15	from	scaffolds	1073:1081	arg1	application					1111:1121	guided bone regeneration application	1086:1121	guided bone regeneration application	1086:1121	Investigation on the biomineralization and cell viability indicated that the inclusion of bioactive mangiferin further promote potential use of the hybrid nanocomposite scaffolds in guided bone regeneration application.
33766383	5	16	theme	guided	1086:1091	arg1	application					1111:1121	guided bone regeneration application	1086:1121	guided bone regeneration application	1086:1121	Investigation on the biomineralization and cell viability indicated that the inclusion of bioactive mangiferin further promote potential use of the hybrid nanocomposite scaffolds in guided bone regeneration application.
33766383	1	17	theme	promising	211:219	arg1	method					221:226	a promising method	209:226	a promising method to enhance the stability and mechanical properties of biopolymers	209:292	Development of hybrid materials with molecular structure of organic-inorganic co-network is a promising method to enhance the stability and mechanical properties of biopolymers.
33766383	1	17	theme	promising	211:219	arg1	Development					117:127	Development	117:127	Development of hybrid materials with molecular structure of organic-inorganic co-network	117:204	Development of hybrid materials with molecular structure of organic-inorganic co-network is a promising method to enhance the stability and mechanical properties of biopolymers.
33766383	5	18	from	application	1111:1121	arg1	use					1041:1043	potential use	1031:1043	potential use of the hybrid nanocomposite scaffolds in guided bone regeneration application	1031:1121	Investigation on the biomineralization and cell viability indicated that the inclusion of bioactive mangiferin further promote potential use of the hybrid nanocomposite scaffolds in guided bone regeneration application.
33766383	2	19	theme	active	382:387	arg1	compound					389:396	a plant-derived active compound	366:396	a plant-derived active compound possessing several bioactivities	366:429	Chitosan-silica hybrid nanocomposite scaffolds loaded with mangiferin, a plant-derived active compound possessing several bioactivities, were fabricated using the sol-gel synthesis and the freeze-drying processes.
33766383	2	19	theme	active	382:387	arg1	mangiferin					354:363	mangiferin	354:363	mangiferin	354:363	Chitosan-silica hybrid nanocomposite scaffolds loaded with mangiferin, a plant-derived active compound possessing several bioactivities, were fabricated using the sol-gel synthesis and the freeze-drying processes.
33766383	2	20	theme	sol-gel	458:464	arg1	synthesis					466:474	the sol-gel synthesis	454:474	the sol-gel synthesis	454:474	Chitosan-silica hybrid nanocomposite scaffolds loaded with mangiferin, a plant-derived active compound possessing several bioactivities, were fabricated using the sol-gel synthesis and the freeze-drying processes.
33766383	5	21	theme	cell	947:950	arg1	viability					952:960	the biomineralization and cell viability	921:960	viability	952:960	Investigation on the biomineralization and cell viability indicated that the inclusion of bioactive mangiferin further promote potential use of the hybrid nanocomposite scaffolds in guided bone regeneration application.
33766383	2	22	theme	plant-derived	368:380	arg1	compound					389:396	a plant-derived active compound	366:396	a plant-derived active compound possessing several bioactivities	366:429	Chitosan-silica hybrid nanocomposite scaffolds loaded with mangiferin, a plant-derived active compound possessing several bioactivities, were fabricated using the sol-gel synthesis and the freeze-drying processes.
33766383	2	22	theme	plant-derived	368:380	arg1	mangiferin					354:363	mangiferin	354:363	mangiferin	354:363	Chitosan-silica hybrid nanocomposite scaffolds loaded with mangiferin, a plant-derived active compound possessing several bioactivities, were fabricated using the sol-gel synthesis and the freeze-drying processes.
33766383	0	23	theme	loaded	26:31	arg1	scaffolds					56:64	mangiferin loaded chitosan-silica hybrid scaffolds	15:64	mangiferin loaded chitosan-silica hybrid scaffolds	15:64	Development of mangiferin loaded chitosan-silica hybrid scaffolds: Physicochemical and bioactivity characterization.
33766383	5	24	theme	hybrid	1052:1057	arg1	scaffolds					1073:1081	the hybrid nanocomposite scaffolds	1048:1081	the hybrid nanocomposite scaffolds in guided bone regeneration application	1048:1121	Investigation on the biomineralization and cell viability indicated that the inclusion of bioactive mangiferin further promote potential use of the hybrid nanocomposite scaffolds in guided bone regeneration application.
33766383	4	25	theme	bone	867:870	arg1	application					891:901	bone tissue engineering application	867:901	bone tissue engineering application	867:901	The scaffolds possessed porosity, fluid uptake, morphology, thermal properties and mechanical strength suitable for bone tissue engineering application.
33766383	1	26	with	Development	117:127	arg1	structure					164:172	molecular structure	154:172	molecular structure of organic-inorganic co-network	154:204	Development of hybrid materials with molecular structure of organic-inorganic co-network is a promising method to enhance the stability and mechanical properties of biopolymers.
33766383	5	27	theme	bone	1093:1096	arg1	application					1111:1121	guided bone regeneration application	1086:1121	guided bone regeneration application	1086:1121	Investigation on the biomineralization and cell viability indicated that the inclusion of bioactive mangiferin further promote potential use of the hybrid nanocomposite scaffolds in guided bone regeneration application.
33766383	1	28	theme	hybrid	132:137	arg1	materials					139:147	hybrid materials	132:147	hybrid materials	132:147	Development of hybrid materials with molecular structure of organic-inorganic co-network is a promising method to enhance the stability and mechanical properties of biopolymers.
33766383	5	29	theme	mangiferin	1004:1013	arg1	inclusion					981:989	the inclusion	977:989	the inclusion of bioactive mangiferin	977:1013	Investigation on the biomineralization and cell viability indicated that the inclusion of bioactive mangiferin further promote potential use of the hybrid nanocomposite scaffolds in guided bone regeneration application.
33766383	0	30	theme	hybrid	49:54	arg1	scaffolds					56:64	mangiferin loaded chitosan-silica hybrid scaffolds	15:64	mangiferin loaded chitosan-silica hybrid scaffolds	15:64	Development of mangiferin loaded chitosan-silica hybrid scaffolds: Physicochemical and bioactivity characterization.
33766383	4	31	theme	thermal	811:817	arg1	properties					819:828	thermal properties	811:828	thermal properties	811:828	The scaffolds possessed porosity, fluid uptake, morphology, thermal properties and mechanical strength suitable for bone tissue engineering application.
33766383	3	32	theme	network	704:710	arg1	addition					720:727	the addition	716:727	the addition of ZnO nanoparticles	716:748	Investigation on the physicochemical and mechanical properties of the fabricated scaffolds showed that their properties can be improved and tailored by the formation of 3-dimensional crosslinked network and the addition of ZnO nanoparticles.
33766383	3	32	theme	network	704:710	arg1	formation					665:673	the formation	661:673	the formation of 3-dimensional crosslinked network	661:710	Investigation on the physicochemical and mechanical properties of the fabricated scaffolds showed that their properties can be improved and tailored by the formation of 3-dimensional crosslinked network and the addition of ZnO nanoparticles.
33766383	2	33	theme	freeze-drying	484:496	arg1	processes					498:506	the freeze-drying processes	480:506	the freeze-drying processes	480:506	Chitosan-silica hybrid nanocomposite scaffolds loaded with mangiferin, a plant-derived active compound possessing several bioactivities, were fabricated using the sol-gel synthesis and the freeze-drying processes.
33766383	0	34	theme	chitosan-silica	33:47	arg1	scaffolds					56:64	mangiferin loaded chitosan-silica hybrid scaffolds	15:64	mangiferin loaded chitosan-silica hybrid scaffolds	15:64	Development of mangiferin loaded chitosan-silica hybrid scaffolds: Physicochemical and bioactivity characterization.
33766383	3	35	link	crosslinked	692:702	arg1	network					704:710	3-dimensional crosslinked network	678:710	3-dimensional crosslinked network	678:710	Investigation on the physicochemical and mechanical properties of the fabricated scaffolds showed that their properties can be improved and tailored by the formation of 3-dimensional crosslinked network and the addition of ZnO nanoparticles.
33766383	3	36	theme	nanoparticles	736:748	arg1	addition					720:727	the addition	716:727	the addition of ZnO nanoparticles	716:748	Investigation on the physicochemical and mechanical properties of the fabricated scaffolds showed that their properties can be improved and tailored by the formation of 3-dimensional crosslinked network and the addition of ZnO nanoparticles.
33766383	3	36	theme	nanoparticles	736:748	arg1	formation					665:673	the formation	661:673	the formation of 3-dimensional crosslinked network	661:710	Investigation on the physicochemical and mechanical properties of the fabricated scaffolds showed that their properties can be improved and tailored by the formation of 3-dimensional crosslinked network and the addition of ZnO nanoparticles.
33766383	3	37	theme	mechanical	550:559	arg1	properties					561:570	the physicochemical and mechanical properties	526:570	the physicochemical and mechanical properties of the fabricated scaffolds	526:598	Investigation on the physicochemical and mechanical properties of the fabricated scaffolds showed that their properties can be improved and tailored by the formation of 3-dimensional crosslinked network and the addition of ZnO nanoparticles.
33766383	3	38	theme	3-dimensional	678:690	arg1	network					704:710	3-dimensional crosslinked network	678:710	3-dimensional crosslinked network	678:710	Investigation on the physicochemical and mechanical properties of the fabricated scaffolds showed that their properties can be improved and tailored by the formation of 3-dimensional crosslinked network and the addition of ZnO nanoparticles.
33766383	1	39	theme	materials	139:147	arg1	method					221:226	a promising method	209:226	a promising method to enhance the stability and mechanical properties of biopolymers	209:292	Development of hybrid materials with molecular structure of organic-inorganic co-network is a promising method to enhance the stability and mechanical properties of biopolymers.
33766383	1	39	theme	materials	139:147	arg1	Development					117:127	Development	117:127	Development of hybrid materials with molecular structure of organic-inorganic co-network	117:204	Development of hybrid materials with molecular structure of organic-inorganic co-network is a promising method to enhance the stability and mechanical properties of biopolymers.
33766383	0	40	theme	scaffolds	56:64	arg1	Development					0:10	Development	0:10	Development of mangiferin loaded chitosan-silica hybrid scaffolds: Physicochemical and bioactivity characterization.	0:115	Development of mangiferin loaded chitosan-silica hybrid scaffolds: Physicochemical and bioactivity characterization.
33766383	3	41	theme	crosslinked	692:702	arg1	network					704:710	3-dimensional crosslinked network	678:710	3-dimensional crosslinked network	678:710	Investigation on the physicochemical and mechanical properties of the fabricated scaffolds showed that their properties can be improved and tailored by the formation of 3-dimensional crosslinked network and the addition of ZnO nanoparticles.
33766383	2	42	theme	several	409:415	arg1	bioactivities					417:429	several bioactivities	409:429	several bioactivities	409:429	Chitosan-silica hybrid nanocomposite scaffolds loaded with mangiferin, a plant-derived active compound possessing several bioactivities, were fabricated using the sol-gel synthesis and the freeze-drying processes.
33766383	2	43	link	plant-derived	368:380	arg1	compound					389:396	a plant-derived active compound	366:396	a plant-derived active compound possessing several bioactivities	366:429	Chitosan-silica hybrid nanocomposite scaffolds loaded with mangiferin, a plant-derived active compound possessing several bioactivities, were fabricated using the sol-gel synthesis and the freeze-drying processes.
33766383	2	43	link	plant-derived	368:380	arg1	mangiferin					354:363	mangiferin	354:363	mangiferin	354:363	Chitosan-silica hybrid nanocomposite scaffolds loaded with mangiferin, a plant-derived active compound possessing several bioactivities, were fabricated using the sol-gel synthesis and the freeze-drying processes.
33766383	2	44	theme	nanocomposite	318:330	arg1	scaffolds					332:340	Chitosan-silica hybrid nanocomposite scaffolds	295:340	Chitosan-silica hybrid nanocomposite scaffolds loaded with mangiferin, a plant-derived active compound possessing several bioactivities,	295:430	Chitosan-silica hybrid nanocomposite scaffolds loaded with mangiferin, a plant-derived active compound possessing several bioactivities, were fabricated using the sol-gel synthesis and the freeze-drying processes.
33766383	1	45	dep	stability	243:251	arg1	the					239:241	the	239:241	the	239:241	Development of hybrid materials with molecular structure of organic-inorganic co-network is a promising method to enhance the stability and mechanical properties of biopolymers.
33766383	1	46	theme	molecular	154:162	arg1	structure					164:172	molecular structure	154:172	molecular structure of organic-inorganic co-network	154:204	Development of hybrid materials with molecular structure of organic-inorganic co-network is a promising method to enhance the stability and mechanical properties of biopolymers.
33766383	5	47	theme	regeneration	1098:1109	arg1	application					1111:1121	guided bone regeneration application	1086:1121	guided bone regeneration application	1086:1121	Investigation on the biomineralization and cell viability indicated that the inclusion of bioactive mangiferin further promote potential use of the hybrid nanocomposite scaffolds in guided bone regeneration application.
33766383	1	48	theme	mechanical	257:266	arg1	properties					268:277	mechanical properties	257:277	mechanical properties	257:277	Development of hybrid materials with molecular structure of organic-inorganic co-network is a promising method to enhance the stability and mechanical properties of biopolymers.
33766383	5	49	theme	scaffolds	1073:1081	arg1	use					1041:1043	potential use	1031:1043	potential use of the hybrid nanocomposite scaffolds in guided bone regeneration application	1031:1121	Investigation on the biomineralization and cell viability indicated that the inclusion of bioactive mangiferin further promote potential use of the hybrid nanocomposite scaffolds in guided bone regeneration application.
33766383	4	50	contain	possessed	765:773	arg2	uptake					791:796	fluid uptake	785:796	fluid uptake	785:796	The scaffolds possessed porosity, fluid uptake, morphology, thermal properties and mechanical strength suitable for bone tissue engineering application.
33766383	4	50	contain	possessed	765:773	arg2	porosity					775:782	porosity	775:782	porosity	775:782	The scaffolds possessed porosity, fluid uptake, morphology, thermal properties and mechanical strength suitable for bone tissue engineering application.
33766383	4	50	contain	possessed	765:773	arg2	morphology					799:808	morphology	799:808	morphology	799:808	The scaffolds possessed porosity, fluid uptake, morphology, thermal properties and mechanical strength suitable for bone tissue engineering application.
33766383	4	50	contain	possessed	765:773	arg2	strength					845:852	mechanical strength	834:852	mechanical strength	834:852	The scaffolds possessed porosity, fluid uptake, morphology, thermal properties and mechanical strength suitable for bone tissue engineering application.
33766383	4	50	contain	possessed	765:773	arg2	properties					819:828	thermal properties	811:828	thermal properties	811:828	The scaffolds possessed porosity, fluid uptake, morphology, thermal properties and mechanical strength suitable for bone tissue engineering application.
33766383	4	50	contain	possessed	765:773	arg1	scaffolds					755:763	The scaffolds	751:763	The scaffolds	751:763	The scaffolds possessed porosity, fluid uptake, morphology, thermal properties and mechanical strength suitable for bone tissue engineering application.
33766383	2	51	theme	hybrid	311:316	arg1	scaffolds					332:340	Chitosan-silica hybrid nanocomposite scaffolds	295:340	Chitosan-silica hybrid nanocomposite scaffolds loaded with mangiferin, a plant-derived active compound possessing several bioactivities,	295:430	Chitosan-silica hybrid nanocomposite scaffolds loaded with mangiferin, a plant-derived active compound possessing several bioactivities, were fabricated using the sol-gel synthesis and the freeze-drying processes.
33766383	3	52	from	Investigation	509:521	arg1	properties					561:570	the physicochemical and mechanical properties	526:570	the physicochemical and mechanical properties of the fabricated scaffolds	526:598	Investigation on the physicochemical and mechanical properties of the fabricated scaffolds showed that their properties can be improved and tailored by the formation of 3-dimensional crosslinked network and the addition of ZnO nanoparticles.
33766383	3	53	theme	fabricated	579:588	arg1	scaffolds					590:598	the fabricated scaffolds	575:598	the fabricated scaffolds	575:598	Investigation on the physicochemical and mechanical properties of the fabricated scaffolds showed that their properties can be improved and tailored by the formation of 3-dimensional crosslinked network and the addition of ZnO nanoparticles.
33766383	4	54	theme	mechanical	834:843	arg1	strength					845:852	mechanical strength	834:852	mechanical strength	834:852	The scaffolds possessed porosity, fluid uptake, morphology, thermal properties and mechanical strength suitable for bone tissue engineering application.
33766383	2	55	theme	Chitosan-silica	295:309	arg1	scaffolds					332:340	Chitosan-silica hybrid nanocomposite scaffolds	295:340	Chitosan-silica hybrid nanocomposite scaffolds loaded with mangiferin, a plant-derived active compound possessing several bioactivities,	295:430	Chitosan-silica hybrid nanocomposite scaffolds loaded with mangiferin, a plant-derived active compound possessing several bioactivities, were fabricated using the sol-gel synthesis and the freeze-drying processes.
33766383	5	56	theme	potential	1031:1039	arg1	use					1041:1043	potential use	1031:1043	potential use of the hybrid nanocomposite scaffolds in guided bone regeneration application	1031:1121	Investigation on the biomineralization and cell viability indicated that the inclusion of bioactive mangiferin further promote potential use of the hybrid nanocomposite scaffolds in guided bone regeneration application.
33766383	3	57	theme	physicochemical	530:544	arg1	properties					561:570	the physicochemical and mechanical properties	526:570	the physicochemical and mechanical properties of the fabricated scaffolds	526:598	Investigation on the physicochemical and mechanical properties of the fabricated scaffolds showed that their properties can be improved and tailored by the formation of 3-dimensional crosslinked network and the addition of ZnO nanoparticles.
33766383	0	58	dep	Development	0:10	arg1	bioactivity					87:97	bioactivity	87:97	bioactivity	87:97	Development of mangiferin loaded chitosan-silica hybrid scaffolds: Physicochemical and bioactivity characterization.
33766383	0	58	dep	Development	0:10	arg1	Physicochemical					67:81	Physicochemical	67:81	Physicochemical	67:81	Development of mangiferin loaded chitosan-silica hybrid scaffolds: Physicochemical and bioactivity characterization.
33766383	1	59	theme	organic-inorganic	177:193	arg1	co-network					195:204	organic-inorganic co-network	177:204	organic-inorganic co-network	177:204	Development of hybrid materials with molecular structure of organic-inorganic co-network is a promising method to enhance the stability and mechanical properties of biopolymers.
33766383	1	60	theme	biopolymers	282:292	arg1	stability					243:251	stability	243:251	stability	243:251	Development of hybrid materials with molecular structure of organic-inorganic co-network is a promising method to enhance the stability and mechanical properties of biopolymers.
33766383	1	60	theme	biopolymers	282:292	arg1	properties					268:277	mechanical properties	257:277	mechanical properties	257:277	Development of hybrid materials with molecular structure of organic-inorganic co-network is a promising method to enhance the stability and mechanical properties of biopolymers.
33533202	0	0	theme	possible	96:103	arg1	applications					105:116	possible applications	96:116	possible applications in cartilage regeneration	96:142	Preparation and characterization of a soluble eggshell membrane/agarose composite scaffold with possible applications in cartilage regeneration.
33533202	10	1	theme	excellent	1366:1374	arg1	biocompatibility					1376:1391	excellent biocompatibility	1366:1391	excellent biocompatibility	1366:1391	Agarose is capable of controlling mechanical properties, has excellent biocompatibility, and is suitable for forming a hydrogel having a three-dimensional porosity.
33533202	1	2	theme	hydrated	189:196	arg1	tissue					216:221	an extremely hydrated, not vascularized tissue	176:221	an extremely hydrated, not vascularized tissue with a low-cell density	176:245	Articular hyaline cartilage is an extremely hydrated, not vascularized tissue with a low-cell density.
33533202	1	2	theme	hydrated	189:196	arg1	cartilage					163:171	Articular hyaline cartilage	145:171	Articular hyaline cartilage	145:171	Articular hyaline cartilage is an extremely hydrated, not vascularized tissue with a low-cell density.
33533202	12	3	theme	messenger	1653:1661	arg1	expression					1667:1676	messenger RNA expression	1653:1676	messenger RNA expression	1653:1676	In in vitro experiment, cytotoxicity, cell proliferation, and messenger RNA expression were investigated.
33533202	8	4	theme	poor	1092:1095	arg1	properties					1108:1117	poor mechanical properties	1092:1117	poor mechanical properties	1092:1117	However, scaffolds made of collagen only has poor mechanical properties.
33533202	10	5	theme	mechanical	1339:1348	arg1	properties					1350:1359	mechanical properties	1339:1359	mechanical properties	1339:1359	Agarose is capable of controlling mechanical properties, has excellent biocompatibility, and is suitable for forming a hydrogel having a three-dimensional porosity.
33533202	2	6	theme	gradual	302:308	arg1	osteoarthritis					328:341	osteoarthritis	328:341	osteoarthritis	328:341	The damage of this tissue can occur after injuries or gradual stress and tears (osteoarthritis), minor damages can be self-healed in several weeks, but major injuries may eventually require surgery.
33533202	2	6	theme	gradual	302:308	arg1	stress					310:315	gradual stress	302:315	gradual stress	302:315	The damage of this tissue can occur after injuries or gradual stress and tears (osteoarthritis), minor damages can be self-healed in several weeks, but major injuries may eventually require surgery.
33533202	11	7	theme	mechanical	1528:1537	arg1	properties					1539:1548	mechanical properties	1528:1548	mechanical properties	1528:1548	The scaffold was examined for Fourier-transform infrared, mechanical properties, biodegradability, and biocompatibility.
33533202	3	8	from	fact	450:453	arg1	difficult					554:562	difficult	554:562	difficult	554:562	In fact, in this case, because of nature of the cartilage (the absence of cells and vascularization) it is difficult to expect its natural regeneration in a reasonable amount of time.
33533202	3	9	dep	cartilage	495:503	arg1	absence					510:516	the absence	506:516	the absence of cells and vascularization	506:545	In fact, in this case, because of nature of the cartilage (the absence of cells and vascularization) it is difficult to expect its natural regeneration in a reasonable amount of time.
33533202	7	10	theme	eggshell	876:883	arg1	membrane					885:892	The eggshell membrane	872:892	The eggshell membrane	872:892	The eggshell membrane is structurally similar to the extracellular matrix and nontoxic due to its many collagen components and has good biocompatibility and biodegradability.
33533202	7	10	theme	eggshell	876:883	arg1	similar					910:916	similar	910:916	similar	910:916	The eggshell membrane is structurally similar to the extracellular matrix and nontoxic due to its many collagen components and has good biocompatibility and biodegradability.
33533202	3	11	theme	cartilage	495:503	arg1	nature					481:486	nature	481:486	nature of the cartilage (the absence of cells and vascularization)	481:546	In fact, in this case, because of nature of the cartilage (the absence of cells and vascularization) it is difficult to expect its natural regeneration in a reasonable amount of time.
33533202	0	12	theme	cartilage	121:129	arg1	regeneration					131:142	cartilage regeneration	121:142	cartilage regeneration	121:142	Preparation and characterization of a soluble eggshell membrane/agarose composite scaffold with possible applications in cartilage regeneration.
33533202	10	13	contain	having	1433:1438	arg2	porosity					1460:1467	a three-dimensional porosity	1440:1467	a three-dimensional porosity	1440:1467	Agarose is capable of controlling mechanical properties, has excellent biocompatibility, and is suitable for forming a hydrogel having a three-dimensional porosity.
33533202	10	13	contain	having	1433:1438	arg1	hydrogel					1424:1431	a hydrogel	1422:1431	a hydrogel having a three-dimensional porosity	1422:1467	Agarose is capable of controlling mechanical properties, has excellent biocompatibility, and is suitable for forming a hydrogel having a three-dimensional porosity.
33533202	12	14	theme	cell	1629:1632	arg1	proliferation					1634:1646	cell proliferation	1629:1646	cell proliferation	1629:1646	In in vitro experiment, cytotoxicity, cell proliferation, and messenger RNA expression were investigated.
33533202	10	15	contain	has	1362:1364	arg2	biocompatibility					1376:1391	excellent biocompatibility	1366:1391	excellent biocompatibility	1366:1391	Agarose is capable of controlling mechanical properties, has excellent biocompatibility, and is suitable for forming a hydrogel having a three-dimensional porosity.
33533202	10	15	contain	has	1362:1364	arg1	Agarose					1305:1311	Agarose	1305:1311	Agarose	1305:1311	Agarose is capable of controlling mechanical properties, has excellent biocompatibility, and is suitable for forming a hydrogel having a three-dimensional porosity.
33533202	7	16	theme	good	1003:1006	arg1	biocompatibility					1008:1023	good biocompatibility	1003:1023	good biocompatibility	1003:1023	The eggshell membrane is structurally similar to the extracellular matrix and nontoxic due to its many collagen components and has good biocompatibility and biodegradability.
33533202	3	17	from	case	464:467	arg1	difficult					554:562	difficult	554:562	difficult	554:562	In fact, in this case, because of nature of the cartilage (the absence of cells and vascularization) it is difficult to expect its natural regeneration in a reasonable amount of time.
33533202	7	18	theme	many	970:973	arg1	components					984:993	its many collagen components	966:993	its many collagen components	966:993	The eggshell membrane is structurally similar to the extracellular matrix and nontoxic due to its many collagen components and has good biocompatibility and biodegradability.
33533202	2	19	theme	several	381:387	arg1	weeks					389:393	several weeks	381:393	several weeks	381:393	The damage of this tissue can occur after injuries or gradual stress and tears (osteoarthritis), minor damages can be self-healed in several weeks, but major injuries may eventually require surgery.
33533202	2	20	from	self-healed	366:376	arg1	weeks					389:393	several weeks	381:393	several weeks	381:393	The damage of this tissue can occur after injuries or gradual stress and tears (osteoarthritis), minor damages can be self-healed in several weeks, but major injuries may eventually require surgery.
33533202	1	21	dep	hydrated	189:196	arg1	vascularized					203:214	vascularized	203:214	vascularized	203:214	Articular hyaline cartilage is an extremely hydrated, not vascularized tissue with a low-cell density.
33533202	12	22	theme	in	1594:1595	arg1	experiment					1603:1612	in vitro experiment	1594:1612	in vitro experiment	1594:1612	In in vitro experiment, cytotoxicity, cell proliferation, and messenger RNA expression were investigated.
33533202	3	23	theme	reasonable	604:613	arg1	time					625:628	time	625:628	time	625:628	In fact, in this case, because of nature of the cartilage (the absence of cells and vascularization) it is difficult to expect its natural regeneration in a reasonable amount of time.
33533202	3	23	theme	reasonable	604:613	arg1	amount					615:620	a reasonable amount	602:620	a reasonable amount of time	602:628	In fact, in this case, because of nature of the cartilage (the absence of cells and vascularization) it is difficult to expect its natural regeneration in a reasonable amount of time.
33533202	13	24	theme	agarose/eggshell	1729:1744	arg1	scaffold					1755:1762	the agarose/eggshell membrane scaffold	1725:1762	the agarose/eggshell membrane scaffold	1725:1762	The study demonstrated that the agarose/eggshell membrane scaffold can be used for chondrocyte transplantation.
33533202	9	25	theme	disulfide	1141:1149	arg1	bond					1151:1154	the disulfide bond	1137:1154	the disulfide bond of collagen extracted from the insoluble eggshell membrane	1137:1213	For this reason, the disulfide bond of collagen extracted from the insoluble eggshell membrane was cut, converted into water-soluble, and then mixed with agarose to prepare a scaffold.
33533202	9	26	theme	collagen	1159:1166	arg1	bond					1151:1154	the disulfide bond	1137:1154	the disulfide bond of collagen extracted from the insoluble eggshell membrane	1137:1213	For this reason, the disulfide bond of collagen extracted from the insoluble eggshell membrane was cut, converted into water-soluble, and then mixed with agarose to prepare a scaffold.
33533202	3	27	theme	vascularization	531:545	arg1	absence					510:516	the absence	506:516	the absence of cells and vascularization	506:545	In fact, in this case, because of nature of the cartilage (the absence of cells and vascularization) it is difficult to expect its natural regeneration in a reasonable amount of time.
33533202	7	28	theme	extracellular	925:937	arg1	matrix					939:944	the extracellular matrix	921:944	the extracellular matrix	921:944	The eggshell membrane is structurally similar to the extracellular matrix and nontoxic due to its many collagen components and has good biocompatibility and biodegradability.
33533202	13	29	used	used	1771:1774	arg2	scaffold					1755:1762	the agarose/eggshell membrane scaffold	1725:1762	the agarose/eggshell membrane scaffold	1725:1762	The study demonstrated that the agarose/eggshell membrane scaffold can be used for chondrocyte transplantation.
33533202	3	30	theme	cells	521:525	arg1	absence					510:516	the absence	506:516	the absence of cells and vascularization	506:545	In fact, in this case, because of nature of the cartilage (the absence of cells and vascularization) it is difficult to expect its natural regeneration in a reasonable amount of time.
33533202	7	31	contain	has	999:1001	arg2	biodegradability					1029:1044	biodegradability	1029:1044	biodegradability	1029:1044	The eggshell membrane is structurally similar to the extracellular matrix and nontoxic due to its many collagen components and has good biocompatibility and biodegradability.
33533202	7	31	contain	has	999:1001	arg1	membrane					885:892	The eggshell membrane	872:892	The eggshell membrane	872:892	The eggshell membrane is structurally similar to the extracellular matrix and nontoxic due to its many collagen components and has good biocompatibility and biodegradability.
33533202	7	31	contain	has	999:1001	arg1	similar					910:916	similar	910:916	similar	910:916	The eggshell membrane is structurally similar to the extracellular matrix and nontoxic due to its many collagen components and has good biocompatibility and biodegradability.
33533202	7	31	contain	has	999:1001	arg2	biocompatibility					1008:1023	good biocompatibility	1003:1023	good biocompatibility	1003:1023	The eggshell membrane is structurally similar to the extracellular matrix and nontoxic due to its many collagen components and has good biocompatibility and biodegradability.
33533202	5	32	theme	implanting	759:768	arg1	chondrocytes					770:781	implanting chondrocytes	759:781	implanting chondrocytes	759:781	In this study, a scaffold for implanting chondrocytes was prepared.
33533202	0	33	theme	soluble	38:44	arg1	scaffold					82:89	a soluble eggshell membrane/agarose composite scaffold	36:89	a soluble eggshell membrane/agarose composite scaffold	36:89	Preparation and characterization of a soluble eggshell membrane/agarose composite scaffold with possible applications in cartilage regeneration.
33533202	4	34	theme	cell	648:651	arg1	therapy					653:659	cell therapy	648:659	cell therapy	648:659	In recent years, cell therapy, in which cells are directly transplanted, has attracted attention.
33533202	7	35	theme	collagen	975:982	arg1	components					984:993	its many collagen components	966:993	its many collagen components	966:993	The eggshell membrane is structurally similar to the extracellular matrix and nontoxic due to its many collagen components and has good biocompatibility and biodegradability.
33533202	1	36	theme	low-cell	230:237	arg1	density					239:245	a low-cell density	228:245	a low-cell density	228:245	Articular hyaline cartilage is an extremely hydrated, not vascularized tissue with a low-cell density.
33533202	3	37	from	difficult	554:562	arg1	fact					450:453	fact	450:453	fact	450:453	In fact, in this case, because of nature of the cartilage (the absence of cells and vascularization) it is difficult to expect its natural regeneration in a reasonable amount of time.
33533202	3	37	from	difficult	554:562	arg1	case					464:467	this case	459:467	this case	459:467	In fact, in this case, because of nature of the cartilage (the absence of cells and vascularization) it is difficult to expect its natural regeneration in a reasonable amount of time.
33533202	2	38	theme	major	400:404	arg1	injuries					406:413	major injuries	400:413	major injuries	400:413	The damage of this tissue can occur after injuries or gradual stress and tears (osteoarthritis), minor damages can be self-healed in several weeks, but major injuries may eventually require surgery.
33533202	8	39	theme	mechanical	1097:1106	arg1	properties					1108:1117	poor mechanical properties	1092:1117	poor mechanical properties	1092:1117	However, scaffolds made of collagen only has poor mechanical properties.
33533202	0	40	theme	membrane/agarose	55:70	arg1	scaffold					82:89	a soluble eggshell membrane/agarose composite scaffold	36:89	a soluble eggshell membrane/agarose composite scaffold	36:89	Preparation and characterization of a soluble eggshell membrane/agarose composite scaffold with possible applications in cartilage regeneration.
33533202	13	41	theme	chondrocyte	1780:1790	arg1	transplantation					1792:1806	chondrocyte transplantation	1780:1806	chondrocyte transplantation	1780:1806	The study demonstrated that the agarose/eggshell membrane scaffold can be used for chondrocyte transplantation.
33533202	0	42	with	characterization	16:31	arg1	applications					105:116	possible applications	96:116	possible applications in cartilage regeneration	96:142	Preparation and characterization of a soluble eggshell membrane/agarose composite scaffold with possible applications in cartilage regeneration.
33533202	2	43	theme	minor	345:349	arg1	damages					351:357	minor damages	345:357	minor damages	345:357	The damage of this tissue can occur after injuries or gradual stress and tears (osteoarthritis), minor damages can be self-healed in several weeks, but major injuries may eventually require surgery.
33533202	0	44	theme	eggshell	46:53	arg1	scaffold					82:89	a soluble eggshell membrane/agarose composite scaffold	36:89	a soluble eggshell membrane/agarose composite scaffold	36:89	Preparation and characterization of a soluble eggshell membrane/agarose composite scaffold with possible applications in cartilage regeneration.
33533202	1	45	with	tissue	216:221	arg1	density					239:245	a low-cell density	228:245	a low-cell density	228:245	Articular hyaline cartilage is an extremely hydrated, not vascularized tissue with a low-cell density.
33533202	3	46	theme	time	625:628	arg1	time					625:628	time	625:628	time	625:628	In fact, in this case, because of nature of the cartilage (the absence of cells and vascularization) it is difficult to expect its natural regeneration in a reasonable amount of time.
33533202	3	46	theme	time	625:628	arg1	amount					615:620	a reasonable amount	602:620	a reasonable amount of time	602:628	In fact, in this case, because of nature of the cartilage (the absence of cells and vascularization) it is difficult to expect its natural regeneration in a reasonable amount of time.
33533202	3	47	theme	nature	481:486	arg1	difficult					554:562	difficult	554:562	difficult	554:562	In fact, in this case, because of nature of the cartilage (the absence of cells and vascularization) it is difficult to expect its natural regeneration in a reasonable amount of time.
33533202	1	48	theme	Articular	145:153	arg1	cartilage					163:171	Articular hyaline cartilage	145:171	Articular hyaline cartilage	145:171	Articular hyaline cartilage is an extremely hydrated, not vascularized tissue with a low-cell density.
33533202	1	48	theme	Articular	145:153	arg1	tissue					216:221	an extremely hydrated, not vascularized tissue	176:221	an extremely hydrated, not vascularized tissue with a low-cell density	176:245	Articular hyaline cartilage is an extremely hydrated, not vascularized tissue with a low-cell density.
33533202	8	49	contain	has	1088:1090	arg2	properties					1108:1117	poor mechanical properties	1092:1117	poor mechanical properties	1092:1117	However, scaffolds made of collagen only has poor mechanical properties.
33533202	8	49	contain	has	1088:1090	arg1	scaffolds					1056:1064	scaffolds	1056:1064	scaffolds made of collagen	1056:1081	However, scaffolds made of collagen only has poor mechanical properties.
33533202	9	50	theme	insoluble	1187:1195	arg1	membrane					1206:1213	the insoluble eggshell membrane	1183:1213	the insoluble eggshell membrane	1183:1213	For this reason, the disulfide bond of collagen extracted from the insoluble eggshell membrane was cut, converted into water-soluble, and then mixed with agarose to prepare a scaffold.
33533202	0	51	with	Preparation	0:10	arg1	applications					105:116	possible applications	96:116	possible applications in cartilage regeneration	96:142	Preparation and characterization of a soluble eggshell membrane/agarose composite scaffold with possible applications in cartilage regeneration.
33533202	1	52	theme	hyaline	155:161	arg1	cartilage					163:171	Articular hyaline cartilage	145:171	Articular hyaline cartilage	145:171	Articular hyaline cartilage is an extremely hydrated, not vascularized tissue with a low-cell density.
33533202	1	52	theme	hyaline	155:161	arg1	tissue					216:221	an extremely hydrated, not vascularized tissue	176:221	an extremely hydrated, not vascularized tissue with a low-cell density	176:245	Articular hyaline cartilage is an extremely hydrated, not vascularized tissue with a low-cell density.
33533202	12	53	theme	RNA	1663:1665	arg1	expression					1667:1676	messenger RNA expression	1653:1676	messenger RNA expression	1653:1676	In in vitro experiment, cytotoxicity, cell proliferation, and messenger RNA expression were investigated.
33533202	2	54	from	weeks	389:393	arg1	self-healed					366:376	self-healed	366:376	self-healed	366:376	The damage of this tissue can occur after injuries or gradual stress and tears (osteoarthritis), minor damages can be self-healed in several weeks, but major injuries may eventually require surgery.
33533202	9	55	theme	eggshell	1197:1204	arg1	membrane					1206:1213	the insoluble eggshell membrane	1183:1213	the insoluble eggshell membrane	1183:1213	For this reason, the disulfide bond of collagen extracted from the insoluble eggshell membrane was cut, converted into water-soluble, and then mixed with agarose to prepare a scaffold.
33533202	3	56	theme	natural	578:584	arg1	regeneration					586:597	its natural regeneration	574:597	its natural regeneration	574:597	In fact, in this case, because of nature of the cartilage (the absence of cells and vascularization) it is difficult to expect its natural regeneration in a reasonable amount of time.
33533202	12	57	dep	in	1594:1595	arg1	vitro					1597:1601	vitro	1597:1601	vitro	1597:1601	In in vitro experiment, cytotoxicity, cell proliferation, and messenger RNA expression were investigated.
33533202	0	58	theme	scaffold	82:89	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of a soluble eggshell membrane/agarose composite scaffold with possible applications in cartilage regeneration.
33533202	0	58	theme	scaffold	82:89	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of a soluble eggshell membrane/agarose composite scaffold with possible applications in cartilage regeneration.
33533202	0	59	from	applications	105:116	arg1	regeneration					131:142	cartilage regeneration	121:142	cartilage regeneration	121:142	Preparation and characterization of a soluble eggshell membrane/agarose composite scaffold with possible applications in cartilage regeneration.
33533202	2	60	theme	tissue	267:272	arg1	damage					252:257	The damage	248:257	The damage of this tissue	248:272	The damage of this tissue can occur after injuries or gradual stress and tears (osteoarthritis), minor damages can be self-healed in several weeks, but major injuries may eventually require surgery.
33533202	4	61	theme	recent	634:639	arg1	years					641:645	recent years	634:645	recent years	634:645	In recent years, cell therapy, in which cells are directly transplanted, has attracted attention.
33533202	10	62	theme	three-dimensional	1442:1458	arg1	porosity					1460:1467	a three-dimensional porosity	1440:1467	a three-dimensional porosity	1440:1467	Agarose is capable of controlling mechanical properties, has excellent biocompatibility, and is suitable for forming a hydrogel having a three-dimensional porosity.
33533202	0	63	theme	composite	72:80	arg1	scaffold					82:89	a soluble eggshell membrane/agarose composite scaffold	36:89	a soluble eggshell membrane/agarose composite scaffold	36:89	Preparation and characterization of a soluble eggshell membrane/agarose composite scaffold with possible applications in cartilage regeneration.
33533202	6	64	theme	eggshell	841:848	arg1	membrane					850:857	the eggshell membrane	837:857	the eggshell membrane	837:857	The scaffold was made as a sponge using the eggshell membrane and agarose.
33533202	13	65	theme	membrane	1746:1753	arg1	scaffold					1755:1762	the agarose/eggshell membrane scaffold	1725:1762	the agarose/eggshell membrane scaffold	1725:1762	The study demonstrated that the agarose/eggshell membrane scaffold can be used for chondrocyte transplantation.
32861701	5	0	theme	initial	1042:1048	arg1	activity					1050:1057	its initial activity	1038:1057	its initial activity	1038:1057	The entrapped SiO2-lipase retained 76.5 % of its initial activity after 30 days of storage at 4 °C while adsorbed and free lipase retained only 43.4 % and 13.7 %, respectively.
32861701	3	1	theme	PVA/Alg	890:896	arg1	hydrogel					898:905	a PVA/Alg hydrogel	888:905	a PVA/Alg hydrogel	888:905	The lipase adsorbed under optimal conditions (SiO2-lipase) was entrapped in a PVA/Alg hydrogel, successfully.
32861701	5	2	theme	activity	1050:1057	arg1	%					1033:1033	76.5 %	1028:1033	76.5 % of its initial activity	1028:1057	The entrapped SiO2-lipase retained 76.5 % of its initial activity after 30 days of storage at 4 °C while adsorbed and free lipase retained only 43.4 % and 13.7 %, respectively.
32861701	5	2	theme	activity	1050:1057	arg1	activity					1050:1057	its initial activity	1038:1057	its initial activity	1038:1057	The entrapped SiO2-lipase retained 76.5 % of its initial activity after 30 days of storage at 4 °C while adsorbed and free lipase retained only 43.4 % and 13.7 %, respectively.
32861701	2	3	theme	20,000	767:772	arg1	activity					725:732	a specific activity	714:732	a specific activity	714:732	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	3	theme	20,000	767:772	arg1	efficiency					753:762	immobilization efficiency	738:762	immobilization efficiency	738:762	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	1	4	theme	SiO2	275:278	arg1	30 mg/mL					313:320	8, 19 and 30 mg/mL	303:320	30 mg/mL	313:320	In this study, the factors affecting lipase adsorption onto SiO2 nanoparticles including SiO2 nanoparticles amounts (8, 19 and 30 mg/mL), lipase concentrations (30, 90 and 150 μg/mL), adsorption temperatures (5, 20 and 35 °C) and adsorption times (1, 12.5 and 24 h) were optimized using central composite design.
32861701	1	4	theme	SiO2	275:278	arg1	8					303:303	8	303:303	8	303:303	In this study, the factors affecting lipase adsorption onto SiO2 nanoparticles including SiO2 nanoparticles amounts (8, 19 and 30 mg/mL), lipase concentrations (30, 90 and 150 μg/mL), adsorption temperatures (5, 20 and 35 °C) and adsorption times (1, 12.5 and 24 h) were optimized using central composite design.
32861701	1	4	theme	SiO2	275:278	arg1	amounts					294:300	SiO2 nanoparticles amounts	275:300	SiO2 nanoparticles amounts (8, 19 and 30 mg/mL)	275:321	In this study, the factors affecting lipase adsorption onto SiO2 nanoparticles including SiO2 nanoparticles amounts (8, 19 and 30 mg/mL), lipase concentrations (30, 90 and 150 μg/mL), adsorption temperatures (5, 20 and 35 °C) and adsorption times (1, 12.5 and 24 h) were optimized using central composite design.
32861701	0	5	theme	silicon	99:105	arg1	nanoparticles					115:127	silicon dioxide nanoparticles	99:127	silicon dioxide nanoparticles	99:127	Improvement of lipase biochemical properties via a two-step immobilization method: Adsorption onto silicon dioxide nanoparticles and entrapment in a polyvinyl alcohol/alginate hydrogel.
32861701	6	6	theme	SiO2-lipase	1170:1180	arg1	activity					1182:1189	SiO2-lipase activity	1170:1189	SiO2-lipase activity	1170:1189	SiO2-lipase activity decreased to 34.43 % after 10 cycles of use, while the entrapped SiO2-lipase retained about 64.59 % of its initial activity.
32861701	2	7	theme	lipase	588:593	arg1	concentration					595:607	a lipase concentration	586:607	a lipase concentration of 106-116 μg/mL	586:624	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	6	8	theme	activity	1306:1313	arg1	%					1289:1289	about 64.59 %	1277:1289	about 64.59 % of its initial activity	1277:1313	SiO2-lipase activity decreased to 34.43 % after 10 cycles of use, while the entrapped SiO2-lipase retained about 64.59 % of its initial activity.
32861701	6	8	theme	activity	1306:1313	arg1	activity					1306:1313	its initial activity	1294:1313	its initial activity	1294:1313	SiO2-lipase activity decreased to 34.43 % after 10 cycles of use, while the entrapped SiO2-lipase retained about 64.59 % of its initial activity.
32861701	1	9	dep	times	427:431	arg1	24 h					446:449	24 h	446:449	24 h	446:449	In this study, the factors affecting lipase adsorption onto SiO2 nanoparticles including SiO2 nanoparticles amounts (8, 19 and 30 mg/mL), lipase concentrations (30, 90 and 150 μg/mL), adsorption temperatures (5, 20 and 35 °C) and adsorption times (1, 12.5 and 24 h) were optimized using central composite design.
32861701	1	9	dep	times	427:431	arg1	12.5					437:440	12.5	437:440	12.5	437:440	In this study, the factors affecting lipase adsorption onto SiO2 nanoparticles including SiO2 nanoparticles amounts (8, 19 and 30 mg/mL), lipase concentrations (30, 90 and 150 μg/mL), adsorption temperatures (5, 20 and 35 °C) and adsorption times (1, 12.5 and 24 h) were optimized using central composite design.
32861701	1	10	theme	nanoparticles	280:292	arg1	30 mg/mL					313:320	8, 19 and 30 mg/mL	303:320	30 mg/mL	313:320	In this study, the factors affecting lipase adsorption onto SiO2 nanoparticles including SiO2 nanoparticles amounts (8, 19 and 30 mg/mL), lipase concentrations (30, 90 and 150 μg/mL), adsorption temperatures (5, 20 and 35 °C) and adsorption times (1, 12.5 and 24 h) were optimized using central composite design.
32861701	1	10	theme	nanoparticles	280:292	arg1	8					303:303	8	303:303	8	303:303	In this study, the factors affecting lipase adsorption onto SiO2 nanoparticles including SiO2 nanoparticles amounts (8, 19 and 30 mg/mL), lipase concentrations (30, 90 and 150 μg/mL), adsorption temperatures (5, 20 and 35 °C) and adsorption times (1, 12.5 and 24 h) were optimized using central composite design.
32861701	1	10	theme	nanoparticles	280:292	arg1	amounts					294:300	SiO2 nanoparticles amounts	275:300	SiO2 nanoparticles amounts (8, 19 and 30 mg/mL)	275:321	In this study, the factors affecting lipase adsorption onto SiO2 nanoparticles including SiO2 nanoparticles amounts (8, 19 and 30 mg/mL), lipase concentrations (30, 90 and 150 μg/mL), adsorption temperatures (5, 20 and 35 °C) and adsorption times (1, 12.5 and 24 h) were optimized using central composite design.
32861701	5	11	from	days	1068:1071	arg1	4 °C					1087:1090	4 °C	1087:1090	4 °C	1087:1090	The entrapped SiO2-lipase retained 76.5 % of its initial activity after 30 days of storage at 4 °C while adsorbed and free lipase retained only 43.4 % and 13.7 %, respectively.
32861701	1	12	theme	central	473:479	arg1	design					491:496	central composite design	473:496	central composite design	473:496	In this study, the factors affecting lipase adsorption onto SiO2 nanoparticles including SiO2 nanoparticles amounts (8, 19 and 30 mg/mL), lipase concentrations (30, 90 and 150 μg/mL), adsorption temperatures (5, 20 and 35 °C) and adsorption times (1, 12.5 and 24 h) were optimized using central composite design.
32861701	2	13	theme	%	795:795	arg1	activity					725:732	a specific activity	714:732	a specific activity	714:732	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	13	theme	%	795:795	arg1	efficiency					753:762	immobilization efficiency	738:762	immobilization efficiency	738:762	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	14	theme	8.5-14 mg/ml	572:583	arg1	time					680:683	an adsorption time	666:683	an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively	666:809	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	14	theme	8.5-14 mg/ml	572:583	arg1	concentration					595:607	a lipase concentration	586:607	a lipase concentration of 106-116 μg/mL	586:624	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	14	theme	8.5-14 mg/ml	572:583	arg1	temperature					641:651	an adsorption temperature	627:651	an adsorption temperature of 20 °C	627:660	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	14	theme	8.5-14 mg/ml	572:583	arg1	amount					562:567	amount	562:567	amount of 8.5-14 mg/ml	562:583	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	14	theme	8.5-14 mg/ml	572:583	arg1	20 °C					656:660	20 °C	656:660	20 °C	656:660	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	14	theme	8.5-14 mg/ml	572:583	arg1	12.5 h					688:693	12.5 h	688:693	12.5 h	688:693	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	14	theme	8.5-14 mg/ml	572:583	arg1	8.5-14 mg/ml					572:583	8.5-14 mg/ml	572:583	8.5-14 mg/ml	572:583	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	14	theme	8.5-14 mg/ml	572:583	arg1	106-116 μg/mL					612:624	106-116 μg/mL	612:624	106-116 μg/mL	612:624	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	1	15	theme	composite	481:489	arg1	design					491:496	central composite design	473:496	central composite design	473:496	In this study, the factors affecting lipase adsorption onto SiO2 nanoparticles including SiO2 nanoparticles amounts (8, 19 and 30 mg/mL), lipase concentrations (30, 90 and 150 μg/mL), adsorption temperatures (5, 20 and 35 °C) and adsorption times (1, 12.5 and 24 h) were optimized using central composite design.
32861701	0	16	theme	dioxide	107:113	arg1	nanoparticles					115:127	silicon dioxide nanoparticles	99:127	silicon dioxide nanoparticles	99:127	Improvement of lipase biochemical properties via a two-step immobilization method: Adsorption onto silicon dioxide nanoparticles and entrapment in a polyvinyl alcohol/alginate hydrogel.
32861701	7	17	theme	free	1328:1331	arg1	lipase					1333:1338	free lipase	1328:1338	free lipase	1328:1338	Compared to free lipase, the Km values increased and decreased for SiO2-lipase and entrapped SiO2-lipase, respectively.
32861701	1	18	theme	adsorption	416:425	arg1	times					427:431	adsorption times	416:431	adsorption times (1, 12.5 and 24 h)	416:450	In this study, the factors affecting lipase adsorption onto SiO2 nanoparticles including SiO2 nanoparticles amounts (8, 19 and 30 mg/mL), lipase concentrations (30, 90 and 150 μg/mL), adsorption temperatures (5, 20 and 35 °C) and adsorption times (1, 12.5 and 24 h) were optimized using central composite design.
32861701	2	19	theme	12.5 h	688:693	arg1	time					680:683	an adsorption time	666:683	an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively	666:809	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	19	theme	12.5 h	688:693	arg1	concentration					595:607	a lipase concentration	586:607	a lipase concentration of 106-116 μg/mL	586:624	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	19	theme	12.5 h	688:693	arg1	temperature					641:651	an adsorption temperature	627:651	an adsorption temperature of 20 °C	627:660	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	19	theme	12.5 h	688:693	arg1	amount					562:567	amount	562:567	amount of 8.5-14 mg/ml	562:583	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	19	theme	12.5 h	688:693	arg1	20 °C					656:660	20 °C	656:660	20 °C	656:660	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	19	theme	12.5 h	688:693	arg1	12.5 h					688:693	12.5 h	688:693	12.5 h	688:693	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	19	theme	12.5 h	688:693	arg1	8.5-14 mg/ml					572:583	8.5-14 mg/ml	572:583	8.5-14 mg/ml	572:583	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	19	theme	12.5 h	688:693	arg1	106-116 μg/mL					612:624	106-116 μg/mL	612:624	106-116 μg/mL	612:624	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	7	20	theme	Km	1345:1346	arg1	values					1348:1353	the Km values	1341:1353	the Km values	1341:1353	Compared to free lipase, the Km values increased and decreased for SiO2-lipase and entrapped SiO2-lipase, respectively.
32861701	8	21	theme	Vmax	1436:1439	arg1	value					1441:1445	Vmax value	1436:1445	Vmax value	1436:1445	Vmax value increased for both SiO2-lipase and entrapped SiO2-lipase.
32861701	5	22	from	4 °C	1087:1090	arg1	storage					1076:1082	storage	1076:1082	storage at 4 °C	1076:1090	The entrapped SiO2-lipase retained 76.5 % of its initial activity after 30 days of storage at 4 °C while adsorbed and free lipase retained only 43.4 % and 13.7 %, respectively.
32861701	5	22	from	4 °C	1087:1090	arg1	days					1068:1071	30 days	1065:1071	30 days of storage at 4 °C	1065:1090	The entrapped SiO2-lipase retained 76.5 % of its initial activity after 30 days of storage at 4 °C while adsorbed and free lipase retained only 43.4 % and 13.7 %, respectively.
32861701	2	23	theme	106-116 μg/mL	612:624	arg1	time					680:683	an adsorption time	666:683	an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively	666:809	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	23	theme	106-116 μg/mL	612:624	arg1	concentration					595:607	a lipase concentration	586:607	a lipase concentration of 106-116 μg/mL	586:624	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	23	theme	106-116 μg/mL	612:624	arg1	temperature					641:651	an adsorption temperature	627:651	an adsorption temperature of 20 °C	627:660	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	23	theme	106-116 μg/mL	612:624	arg1	amount					562:567	amount	562:567	amount of 8.5-14 mg/ml	562:583	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	23	theme	106-116 μg/mL	612:624	arg1	20 °C					656:660	20 °C	656:660	20 °C	656:660	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	23	theme	106-116 μg/mL	612:624	arg1	12.5 h					688:693	12.5 h	688:693	12.5 h	688:693	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	23	theme	106-116 μg/mL	612:624	arg1	8.5-14 mg/ml					572:583	8.5-14 mg/ml	572:583	8.5-14 mg/ml	572:583	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	23	theme	106-116 μg/mL	612:624	arg1	106-116 μg/mL					612:624	106-116 μg/mL	612:624	106-116 μg/mL	612:624	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	24	theme	specific	716:723	arg1	activity					725:732	a specific activity	714:732	a specific activity	714:732	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	8	25	dep	SiO2-lipase	1466:1476	arg1	SiO2-lipase					1492:1502	SiO2-lipase	1492:1502	SiO2-lipase	1492:1502	Vmax value increased for both SiO2-lipase and entrapped SiO2-lipase.
32861701	0	26	theme	biochemical	22:32	arg1	properties					34:43	lipase biochemical properties	15:43	lipase biochemical properties	15:43	Improvement of lipase biochemical properties via a two-step immobilization method: Adsorption onto silicon dioxide nanoparticles and entrapment in a polyvinyl alcohol/alginate hydrogel.
32861701	6	27	theme	use	1231:1233	arg1	cycles					1221:1226	10 cycles	1218:1226	10 cycles of use	1218:1233	SiO2-lipase activity decreased to 34.43 % after 10 cycles of use, while the entrapped SiO2-lipase retained about 64.59 % of its initial activity.
32861701	7	28	dep	SiO2-lipase	1383:1393	arg1	SiO2-lipase					1409:1419	SiO2-lipase	1409:1419	SiO2-lipase	1409:1419	Compared to free lipase, the Km values increased and decreased for SiO2-lipase and entrapped SiO2-lipase, respectively.
32861701	0	29	theme	lipase	15:20	arg1	properties					34:43	lipase biochemical properties	15:43	lipase biochemical properties	15:43	Improvement of lipase biochemical properties via a two-step immobilization method: Adsorption onto silicon dioxide nanoparticles and entrapment in a polyvinyl alcohol/alginate hydrogel.
32861701	0	30	from	Adsorption	83:92	arg1	hydrogel					176:183	a polyvinyl alcohol/alginate hydrogel	147:183	a polyvinyl alcohol/alginate hydrogel	147:183	Improvement of lipase biochemical properties via a two-step immobilization method: Adsorption onto silicon dioxide nanoparticles and entrapment in a polyvinyl alcohol/alginate hydrogel.
32861701	3	31	theme	optimal	838:844	arg1	conditions					846:855	optimal conditions	838:855	optimal conditions (SiO2-lipase)	838:869	The lipase adsorbed under optimal conditions (SiO2-lipase) was entrapped in a PVA/Alg hydrogel, successfully.
32861701	3	31	theme	optimal	838:844	arg1	SiO2-lipase					858:868	SiO2-lipase	858:868	SiO2-lipase	858:868	The lipase adsorbed under optimal conditions (SiO2-lipase) was entrapped in a PVA/Alg hydrogel, successfully.
32861701	6	32	theme	initial	1298:1304	arg1	activity					1306:1313	its initial activity	1294:1313	its initial activity	1294:1313	SiO2-lipase activity decreased to 34.43 % after 10 cycles of use, while the entrapped SiO2-lipase retained about 64.59 % of its initial activity.
32861701	5	33	theme	adsorbed	1098:1105	arg1	lipase					1116:1121	adsorbed and free lipase	1098:1121	adsorbed and free lipase	1098:1121	The entrapped SiO2-lipase retained 76.5 % of its initial activity after 30 days of storage at 4 °C while adsorbed and free lipase retained only 43.4 % and 13.7 %, respectively.
32861701	4	34	theme	immobilization	977:990	arg1	method					960:965	the two-step method	947:965	the two-step method of lipase immobilization	947:990	FESEM and FTIR confirmed the two-step method of lipase immobilization.
32861701	4	35	theme	two-step	951:958	arg1	method					960:965	the two-step method	947:965	the two-step method of lipase immobilization	947:990	FESEM and FTIR confirmed the two-step method of lipase immobilization.
32861701	0	36	theme	properties	34:43	arg1	Improvement					0:10	Improvement	0:10	Improvement of lipase biochemical properties via a two-step immobilization method: Adsorption onto silicon dioxide nanoparticles and entrapment in a polyvinyl alcohol/alginate hydrogel.	0:184	Improvement of lipase biochemical properties via a two-step immobilization method: Adsorption onto silicon dioxide nanoparticles and entrapment in a polyvinyl alcohol/alginate hydrogel.
32861701	5	37	theme	free	1111:1114	arg1	lipase					1116:1121	adsorbed and free lipase	1098:1121	adsorbed and free lipase	1098:1121	The entrapped SiO2-lipase retained 76.5 % of its initial activity after 30 days of storage at 4 °C while adsorbed and free lipase retained only 43.4 % and 13.7 %, respectively.
32861701	1	38	theme	adsorption	370:379	arg1	temperatures					381:392	adsorption temperatures	370:392	adsorption temperatures (5, 20 and 35 °C)	370:410	In this study, the factors affecting lipase adsorption onto SiO2 nanoparticles including SiO2 nanoparticles amounts (8, 19 and 30 mg/mL), lipase concentrations (30, 90 and 150 μg/mL), adsorption temperatures (5, 20 and 35 °C) and adsorption times (1, 12.5 and 24 h) were optimized using central composite design.
32861701	0	39	from	entrapment	133:142	arg1	hydrogel					176:183	a polyvinyl alcohol/alginate hydrogel	147:183	a polyvinyl alcohol/alginate hydrogel	147:183	Improvement of lipase biochemical properties via a two-step immobilization method: Adsorption onto silicon dioxide nanoparticles and entrapment in a polyvinyl alcohol/alginate hydrogel.
32861701	2	40	theme	20 °C	656:660	arg1	time					680:683	an adsorption time	666:683	an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively	666:809	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	40	theme	20 °C	656:660	arg1	concentration					595:607	a lipase concentration	586:607	a lipase concentration of 106-116 μg/mL	586:624	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	40	theme	20 °C	656:660	arg1	temperature					641:651	an adsorption temperature	627:651	an adsorption temperature of 20 °C	627:660	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	40	theme	20 °C	656:660	arg1	amount					562:567	amount	562:567	amount of 8.5-14 mg/ml	562:583	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	40	theme	20 °C	656:660	arg1	20 °C					656:660	20 °C	656:660	20 °C	656:660	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	40	theme	20 °C	656:660	arg1	12.5 h					688:693	12.5 h	688:693	12.5 h	688:693	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	40	theme	20 °C	656:660	arg1	8.5-14 mg/ml					572:583	8.5-14 mg/ml	572:583	8.5-14 mg/ml	572:583	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	40	theme	20 °C	656:660	arg1	106-116 μg/mL					612:624	106-116 μg/mL	612:624	106-116 μg/mL	612:624	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	1	41	theme	lipase	223:228	arg1	adsorption					230:239	lipase adsorption	223:239	lipase adsorption onto SiO2 nanoparticles including SiO2 nanoparticles amounts (8, 19 and 30 mg/mL), lipase concentrations (30, 90 and 150 μg/mL), adsorption temperatures (5, 20 and 35 °C) and adsorption times (1, 12.5 and 24 h)	223:450	In this study, the factors affecting lipase adsorption onto SiO2 nanoparticles including SiO2 nanoparticles amounts (8, 19 and 30 mg/mL), lipase concentrations (30, 90 and 150 μg/mL), adsorption temperatures (5, 20 and 35 °C) and adsorption times (1, 12.5 and 24 h) were optimized using central composite design.
32861701	0	42	theme	two-step	51:58	arg1	method					75:80	a two-step immobilization method	49:80	a two-step immobilization method	49:80	Improvement of lipase biochemical properties via a two-step immobilization method: Adsorption onto silicon dioxide nanoparticles and entrapment in a polyvinyl alcohol/alginate hydrogel.
32861701	6	43	theme	entrapped	1246:1254	arg1	SiO2-lipase					1256:1266	the entrapped SiO2-lipase	1242:1266	the entrapped SiO2-lipase	1242:1266	SiO2-lipase activity decreased to 34.43 % after 10 cycles of use, while the entrapped SiO2-lipase retained about 64.59 % of its initial activity.
32861701	0	44	theme	alcohol/alginate	159:174	arg1	hydrogel					176:183	a polyvinyl alcohol/alginate hydrogel	147:183	a polyvinyl alcohol/alginate hydrogel	147:183	Improvement of lipase biochemical properties via a two-step immobilization method: Adsorption onto silicon dioxide nanoparticles and entrapment in a polyvinyl alcohol/alginate hydrogel.
32861701	0	45	theme	polyvinyl	149:157	arg1	hydrogel					176:183	a polyvinyl alcohol/alginate hydrogel	147:183	a polyvinyl alcohol/alginate hydrogel	147:183	Improvement of lipase biochemical properties via a two-step immobilization method: Adsorption onto silicon dioxide nanoparticles and entrapment in a polyvinyl alcohol/alginate hydrogel.
32861701	5	46	theme	entrapped	997:1005	arg1	SiO2-lipase					1007:1017	The entrapped SiO2-lipase	993:1017	The entrapped SiO2-lipase	993:1017	The entrapped SiO2-lipase retained 76.5 % of its initial activity after 30 days of storage at 4 °C while adsorbed and free lipase retained only 43.4 % and 13.7 %, respectively.
32861701	5	47	theme	storage	1076:1082	arg1	days					1068:1071	30 days	1065:1071	30 days of storage at 4 °C	1065:1090	The entrapped SiO2-lipase retained 76.5 % of its initial activity after 30 days of storage at 4 °C while adsorbed and free lipase retained only 43.4 % and 13.7 %, respectively.
32861701	2	48	theme	optimal	503:509	arg1	conditions					511:520	The optimal conditions	499:520	The optimal conditions	499:520	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	49	theme	adsorption	630:639	arg1	temperature					641:651	an adsorption temperature	627:651	an adsorption temperature of 20 °C	627:660	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	50	theme	immobilization	738:751	arg1	efficiency					753:762	immobilization efficiency	738:762	immobilization efficiency	738:762	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	1	51	dep	temperatures	381:392	arg1	35 °C					405:409	35 °C	405:409	35 °C	405:409	In this study, the factors affecting lipase adsorption onto SiO2 nanoparticles including SiO2 nanoparticles amounts (8, 19 and 30 mg/mL), lipase concentrations (30, 90 and 150 μg/mL), adsorption temperatures (5, 20 and 35 °C) and adsorption times (1, 12.5 and 24 h) were optimized using central composite design.
32861701	1	51	dep	temperatures	381:392	arg1	20					398:399	20	398:399	20	398:399	In this study, the factors affecting lipase adsorption onto SiO2 nanoparticles including SiO2 nanoparticles amounts (8, 19 and 30 mg/mL), lipase concentrations (30, 90 and 150 μg/mL), adsorption temperatures (5, 20 and 35 °C) and adsorption times (1, 12.5 and 24 h) were optimized using central composite design.
32861701	4	52	theme	lipase	970:975	arg1	immobilization					977:990	lipase immobilization	970:990	lipase immobilization	970:990	FESEM and FTIR confirmed the two-step method of lipase immobilization.
32861701	2	53	theme	adsorption	669:678	arg1	time					680:683	an adsorption time	666:683	an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively	666:809	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	1	54	theme	SiO2	246:249	arg1	times					427:431	adsorption times	416:431	adsorption times (1, 12.5 and 24 h)	416:450	In this study, the factors affecting lipase adsorption onto SiO2 nanoparticles including SiO2 nanoparticles amounts (8, 19 and 30 mg/mL), lipase concentrations (30, 90 and 150 μg/mL), adsorption temperatures (5, 20 and 35 °C) and adsorption times (1, 12.5 and 24 h) were optimized using central composite design.
32861701	1	54	theme	SiO2	246:249	arg1	nanoparticles					251:263	SiO2 nanoparticles	246:263	SiO2 nanoparticles including SiO2 nanoparticles amounts (8, 19 and 30 mg/mL), lipase concentrations (30, 90 and 150 μg/mL), adsorption temperatures (5, 20 and 35 °C) and adsorption times (1, 12.5 and 24 h)	246:450	In this study, the factors affecting lipase adsorption onto SiO2 nanoparticles including SiO2 nanoparticles amounts (8, 19 and 30 mg/mL), lipase concentrations (30, 90 and 150 μg/mL), adsorption temperatures (5, 20 and 35 °C) and adsorption times (1, 12.5 and 24 h) were optimized using central composite design.
32861701	1	54	theme	SiO2	246:249	arg1	temperatures					381:392	adsorption temperatures	370:392	adsorption temperatures (5, 20 and 35 °C)	370:410	In this study, the factors affecting lipase adsorption onto SiO2 nanoparticles including SiO2 nanoparticles amounts (8, 19 and 30 mg/mL), lipase concentrations (30, 90 and 150 μg/mL), adsorption temperatures (5, 20 and 35 °C) and adsorption times (1, 12.5 and 24 h) were optimized using central composite design.
32861701	1	54	theme	SiO2	246:249	arg1	concentrations					331:344	lipase concentrations	324:344	lipase concentrations (30, 90 and 150 μg/mL)	324:367	In this study, the factors affecting lipase adsorption onto SiO2 nanoparticles including SiO2 nanoparticles amounts (8, 19 and 30 mg/mL), lipase concentrations (30, 90 and 150 μg/mL), adsorption temperatures (5, 20 and 35 °C) and adsorption times (1, 12.5 and 24 h) were optimized using central composite design.
32861701	1	54	theme	SiO2	246:249	arg1	amounts					294:300	SiO2 nanoparticles amounts	275:300	SiO2 nanoparticles amounts (8, 19 and 30 mg/mL)	275:321	In this study, the factors affecting lipase adsorption onto SiO2 nanoparticles including SiO2 nanoparticles amounts (8, 19 and 30 mg/mL), lipase concentrations (30, 90 and 150 μg/mL), adsorption temperatures (5, 20 and 35 °C) and adsorption times (1, 12.5 and 24 h) were optimized using central composite design.
32861701	0	55	theme	immobilization	60:73	arg1	method					75:80	a two-step immobilization method	49:80	a two-step immobilization method	49:80	Improvement of lipase biochemical properties via a two-step immobilization method: Adsorption onto silicon dioxide nanoparticles and entrapment in a polyvinyl alcohol/alginate hydrogel.
32861701	1	56	theme	lipase	324:329	arg1	30					347:348	30	347:348	30	347:348	In this study, the factors affecting lipase adsorption onto SiO2 nanoparticles including SiO2 nanoparticles amounts (8, 19 and 30 mg/mL), lipase concentrations (30, 90 and 150 μg/mL), adsorption temperatures (5, 20 and 35 °C) and adsorption times (1, 12.5 and 24 h) were optimized using central composite design.
32861701	1	56	theme	lipase	324:329	arg1	concentrations					331:344	lipase concentrations	324:344	lipase concentrations (30, 90 and 150 μg/mL)	324:367	In this study, the factors affecting lipase adsorption onto SiO2 nanoparticles including SiO2 nanoparticles amounts (8, 19 and 30 mg/mL), lipase concentrations (30, 90 and 150 μg/mL), adsorption temperatures (5, 20 and 35 °C) and adsorption times (1, 12.5 and 24 h) were optimized using central composite design.
32861701	1	56	theme	lipase	324:329	arg1	150 μg/mL					358:366	30, 90 and 150 μg/mL	347:366	150 μg/mL	358:366	In this study, the factors affecting lipase adsorption onto SiO2 nanoparticles including SiO2 nanoparticles amounts (8, 19 and 30 mg/mL), lipase concentrations (30, 90 and 150 μg/mL), adsorption temperatures (5, 20 and 35 °C) and adsorption times (1, 12.5 and 24 h) were optimized using central composite design.
32861701	0	57	dep	Improvement	0:10	arg1	Adsorption					83:92	Adsorption	83:92	Adsorption onto silicon dioxide nanoparticles	83:127	Improvement of lipase biochemical properties via a two-step immobilization method: Adsorption onto silicon dioxide nanoparticles and entrapment in a polyvinyl alcohol/alginate hydrogel.
32861701	0	57	dep	Improvement	0:10	arg1	entrapment					133:142	entrapment	133:142	entrapment in a polyvinyl alcohol/alginate hydrogel	133:183	Improvement of lipase biochemical properties via a two-step immobilization method: Adsorption onto silicon dioxide nanoparticles and entrapment in a polyvinyl alcohol/alginate hydrogel.
32861701	2	58	theme	U/g	775:777	arg1	20,000					767:772	20,000	767:772	20,000	767:772	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
32861701	2	58	theme	U/g	775:777	arg1	protein					779:785	U/g protein	775:785	U/g protein	775:785	The optimal conditions were determined as a SiO2 nanoparticles amount of 8.5-14 mg/ml, a lipase concentration of 106-116 μg/mL, an adsorption temperature of 20 °C and an adsorption time of 12.5 h, which resulted in a specific activity and immobilization efficiency of 20,000 (U/g protein) and 60 %, respectively.
34830249	4	0	theme	components	543:552	arg1	one					526:528	one	526:528	one	526:528	Albumin is one of the major components in synovial fluid.
34830249	4	0	theme	components	543:552	arg1	components					543:552	the major components	533:552	the major components in synovial fluid	533:570	Albumin is one of the major components in synovial fluid.
34830249	6	1	theme	fluid	745:749	arg1	context					725:731	the context	721:731	the context of synovial fluid	721:749	In the context of synovial fluid, it is relevant to describe the possible interactions between albumin and hyaluronate, taking into account solution composition effects.
34830249	10	2	dep	exhibit	1410:1416	arg1	direct					1479:1484	direct	1479:1484	direct	1479:1484	The indicated moieties exhibit a locally positive charge which enables hyaluronate binding (direct and water mediated).
34830249	10	2	dep	exhibit	1410:1416	arg1	mediated					1496:1503	mediated	1496:1503	mediated	1496:1503	The indicated moieties exhibit a locally positive charge which enables hyaluronate binding (direct and water mediated).
34830249	7	3	theme	serum	954:958	arg1	interactions					979:990	human serum albumin-hyaluronan interactions	948:990	human serum albumin-hyaluronan interactions	948:990	In this study, the influence of Na+, Mg2+, and Ca2+ ions on human serum albumin-hyaluronan interactions were examined using molecular dynamics tools.
34830249	8	4	theme	affinity	1159:1166	arg1	increase					1143:1150	the increase	1139:1150	the increase of the affinity between hyaluronan and albumin, which is associated with charge compensation in negatively charged hyaluronan and albumin	1139:1288	It was established that the presence of divalent cations, and especially Ca2+, contributes mostly to the increase of the affinity between hyaluronan and albumin, which is associated with charge compensation in negatively charged hyaluronan and albumin.
34830249	4	5	theme	synovial	557:564	arg1	fluid					566:570	synovial fluid	557:570	synovial fluid	557:570	Albumin is one of the major components in synovial fluid.
34830249	7	6	theme	albumin-hyaluronan	960:977	arg1	interactions					979:990	human serum albumin-hyaluronan interactions	948:990	human serum albumin-hyaluronan interactions	948:990	In this study, the influence of Na+, Mg2+, and Ca2+ ions on human serum albumin-hyaluronan interactions were examined using molecular dynamics tools.
34830249	6	7	from	context	725:731	arg1	relevant					758:765	relevant	758:765	relevant	758:765	In the context of synovial fluid, it is relevant to describe the possible interactions between albumin and hyaluronate, taking into account solution composition effects.
34830249	9	8	theme	most	1308:1311	arg1	sites					1330:1334	the most probable binding sites	1304:1334	the most probable binding sites	1304:1334	Furthermore, the most probable binding sites were structurally and energetically characterized.
34830249	3	9	theme	important	398:406	arg1	tool					408:411	An important tool	395:411	An important tool for characterizing the intermolecular forces and their structural consequences	395:490	An important tool for characterizing the intermolecular forces and their structural consequences is molecular dynamics.
34830249	3	9	theme	important	398:406	arg1	dynamics					505:512	molecular dynamics	495:512	molecular dynamics	495:512	An important tool for characterizing the intermolecular forces and their structural consequences is molecular dynamics.
34830249	9	10	theme	probable	1313:1320	arg1	sites					1330:1334	the most probable binding sites	1304:1334	the most probable binding sites	1304:1334	Furthermore, the most probable binding sites were structurally and energetically characterized.
34830249	4	11	from	components	543:552	arg1	fluid					566:570	synovial fluid	557:570	synovial fluid	557:570	Albumin is one of the major components in synovial fluid.
34830249	2	12	theme	inorganic	312:320	arg1	ions					322:325	simple inorganic ions	305:325	simple inorganic ions	305:325	Nevertheless, intermolecular interactions between various neutral and ionic species including large macromolecular systems and simple inorganic ions are the key to understanding the excellent lubrication performance.
34830249	6	13	theme	synovial	736:743	arg1	fluid					745:749	synovial fluid	736:749	synovial fluid	736:749	In the context of synovial fluid, it is relevant to describe the possible interactions between albumin and hyaluronate, taking into account solution composition effects.
34830249	2	14	theme	lubrication	370:380	arg1	performance					382:392	the excellent lubrication performance	356:392	the excellent lubrication performance	356:392	Nevertheless, intermolecular interactions between various neutral and ionic species including large macromolecular systems and simple inorganic ions are the key to understanding the excellent lubrication performance.
34830249	7	15	theme	human	948:952	arg1	interactions					979:990	human serum albumin-hyaluronan interactions	948:990	human serum albumin-hyaluronan interactions	948:990	In this study, the influence of Na+, Mg2+, and Ca2+ ions on human serum albumin-hyaluronan interactions were examined using molecular dynamics tools.
34830249	2	16	theme	simple	305:310	arg1	ions					322:325	simple inorganic ions	305:325	simple inorganic ions	305:325	Nevertheless, intermolecular interactions between various neutral and ionic species including large macromolecular systems and simple inorganic ions are the key to understanding the excellent lubrication performance.
34830249	8	17	theme	charged	1259:1265	arg1	hyaluronan					1267:1276	hyaluronan	1267:1276	hyaluronan	1267:1276	It was established that the presence of divalent cations, and especially Ca2+, contributes mostly to the increase of the affinity between hyaluronan and albumin, which is associated with charge compensation in negatively charged hyaluronan and albumin.
34830249	4	18	theme	major	537:541	arg1	components					543:552	the major components	533:552	the major components in synovial fluid	533:570	Albumin is one of the major components in synovial fluid.
34830249	7	19	theme	Ca2+	935:938	arg1	ions					940:943	Na+, Mg2+, and Ca2+ ions	920:943	Na+, Mg2+, and Ca2+ ions	920:943	In this study, the influence of Na+, Mg2+, and Ca2+ ions on human serum albumin-hyaluronan interactions were examined using molecular dynamics tools.
34830249	7	20	from	influence	907:915	arg1	interactions					979:990	human serum albumin-hyaluronan interactions	948:990	human serum albumin-hyaluronan interactions	948:990	In this study, the influence of Na+, Mg2+, and Ca2+ ions on human serum albumin-hyaluronan interactions were examined using molecular dynamics tools.
34830249	3	21	theme	molecular	495:503	arg1	tool					408:411	An important tool	395:411	An important tool for characterizing the intermolecular forces and their structural consequences	395:490	An important tool for characterizing the intermolecular forces and their structural consequences is molecular dynamics.
34830249	3	21	theme	molecular	495:503	arg1	dynamics					505:512	molecular dynamics	495:512	molecular dynamics	495:512	An important tool for characterizing the intermolecular forces and their structural consequences is molecular dynamics.
34830249	10	22	theme	hyaluronate	1458:1468	arg1	binding					1470:1476	hyaluronate binding	1458:1476	hyaluronate binding	1458:1476	The indicated moieties exhibit a locally positive charge which enables hyaluronate binding (direct and water mediated).
34830249	8	23	theme	divalent	1078:1085	arg1	cations					1087:1093	divalent cations	1078:1093	divalent cations	1078:1093	It was established that the presence of divalent cations, and especially Ca2+, contributes mostly to the increase of the affinity between hyaluronan and albumin, which is associated with charge compensation in negatively charged hyaluronan and albumin.
34830249	7	24	theme	ions	940:943	arg1	influence					907:915	the influence	903:915	the influence of Na+, Mg2+, and Ca2+ ions on human serum albumin-hyaluronan interactions	903:990	In this study, the influence of Na+, Mg2+, and Ca2+ ions on human serum albumin-hyaluronan interactions were examined using molecular dynamics tools.
34830249	9	25	theme	binding	1322:1328	arg1	sites					1330:1334	the most probable binding sites	1304:1334	the most probable binding sites	1304:1334	Furthermore, the most probable binding sites were structurally and energetically characterized.
34830249	3	26	theme	structural	468:477	arg1	consequences					479:490	their structural consequences	462:490	their structural consequences	462:490	An important tool for characterizing the intermolecular forces and their structural consequences is molecular dynamics.
34830249	0	27	theme	Albumin-Hyaluronan	0:17	arg1	Interactions					19:30	Albumin-Hyaluronan Interactions	0:30	Albumin-Hyaluronan Interactions	0:30	Albumin-Hyaluronan Interactions: Influence of Ionic Composition Probed by Molecular Dynamics.
34830249	6	28	dep	account	850:856	arg1	effects					879:885	solution composition effects	858:885	account solution composition effects	850:885	In the context of synovial fluid, it is relevant to describe the possible interactions between albumin and hyaluronate, taking into account solution composition effects.
34830249	5	29	theme	molecular	633:641	arg1	complexes					643:651	molecular complexes	633:651	molecular complexes	633:651	Its electrostatic properties, including the ability to form molecular complexes, are closely related to pH, solvation, and the presence of ions.
34830249	1	30	theme	lubrication	98:108	arg1	mechanism					110:118	The lubrication mechanism	94:118	The lubrication mechanism in synovial fluid and joints	94:147	The lubrication mechanism in synovial fluid and joints is not yet fully understood.
34830249	6	31	theme	possible	783:790	arg1	interactions					792:803	the possible interactions	779:803	the possible interactions between albumin and hyaluronate	779:835	In the context of synovial fluid, it is relevant to describe the possible interactions between albumin and hyaluronate, taking into account solution composition effects.
34830249	10	32	theme	positive	1428:1435	arg1	charge					1437:1442	a locally positive charge	1418:1442	a locally positive charge which enables hyaluronate binding	1418:1476	The indicated moieties exhibit a locally positive charge which enables hyaluronate binding (direct and water mediated).
34830249	6	33	theme	composition	867:877	arg1	effects					879:885	solution composition effects	858:885	account solution composition effects	850:885	In the context of synovial fluid, it is relevant to describe the possible interactions between albumin and hyaluronate, taking into account solution composition effects.
34830249	8	34	theme	cations	1087:1093	arg1	presence					1066:1073	the presence	1062:1073	the presence of divalent cations	1062:1093	It was established that the presence of divalent cations, and especially Ca2+, contributes mostly to the increase of the affinity between hyaluronan and albumin, which is associated with charge compensation in negatively charged hyaluronan and albumin.
34830249	0	35	theme	Ionic	46:50	arg1	Composition					52:62	Ionic Composition	46:62	Ionic Composition	46:62	Albumin-Hyaluronan Interactions: Influence of Ionic Composition Probed by Molecular Dynamics.
34830249	6	36	from	relevant	758:765	arg1	context					725:731	the context	721:731	the context of synovial fluid	721:749	In the context of synovial fluid, it is relevant to describe the possible interactions between albumin and hyaluronate, taking into account solution composition effects.
34830249	2	37	theme	ionic	248:252	arg1	ions					322:325	simple inorganic ions	305:325	simple inorganic ions	305:325	Nevertheless, intermolecular interactions between various neutral and ionic species including large macromolecular systems and simple inorganic ions are the key to understanding the excellent lubrication performance.
34830249	2	37	theme	ionic	248:252	arg1	species					254:260	various neutral and ionic species	228:260	various neutral and ionic species including large macromolecular systems and simple inorganic ions	228:325	Nevertheless, intermolecular interactions between various neutral and ionic species including large macromolecular systems and simple inorganic ions are the key to understanding the excellent lubrication performance.
34830249	2	37	theme	ionic	248:252	arg1	systems					293:299	large macromolecular systems	272:299	large macromolecular systems	272:299	Nevertheless, intermolecular interactions between various neutral and ionic species including large macromolecular systems and simple inorganic ions are the key to understanding the excellent lubrication performance.
34830249	3	38	theme	intermolecular	436:449	arg1	forces					451:456	the intermolecular forces	432:456	the intermolecular forces	432:456	An important tool for characterizing the intermolecular forces and their structural consequences is molecular dynamics.
34830249	1	39	theme	synovial	123:130	arg1	fluid					132:136	synovial fluid	123:136	synovial fluid	123:136	The lubrication mechanism in synovial fluid and joints is not yet fully understood.
34830249	0	40	theme	Composition	52:62	arg1	Influence					33:41	Influence	33:41	Influence of Ionic Composition Probed by Molecular Dynamics	33:91	Albumin-Hyaluronan Interactions: Influence of Ionic Composition Probed by Molecular Dynamics.
34830249	5	41	theme	ions	712:715	arg1	solvation					681:689	solvation	681:689	solvation	681:689	Its electrostatic properties, including the ability to form molecular complexes, are closely related to pH, solvation, and the presence of ions.
34830249	5	41	theme	ions	712:715	arg1	pH					677:678	pH	677:678	pH	677:678	Its electrostatic properties, including the ability to form molecular complexes, are closely related to pH, solvation, and the presence of ions.
34830249	5	41	theme	ions	712:715	arg1	presence					700:707	the presence	696:707	the presence of ions	696:715	Its electrostatic properties, including the ability to form molecular complexes, are closely related to pH, solvation, and the presence of ions.
34830249	2	42	theme	various	228:234	arg1	ions					322:325	simple inorganic ions	305:325	simple inorganic ions	305:325	Nevertheless, intermolecular interactions between various neutral and ionic species including large macromolecular systems and simple inorganic ions are the key to understanding the excellent lubrication performance.
34830249	2	42	theme	various	228:234	arg1	species					254:260	various neutral and ionic species	228:260	various neutral and ionic species including large macromolecular systems and simple inorganic ions	228:325	Nevertheless, intermolecular interactions between various neutral and ionic species including large macromolecular systems and simple inorganic ions are the key to understanding the excellent lubrication performance.
34830249	2	42	theme	various	228:234	arg1	systems					293:299	large macromolecular systems	272:299	large macromolecular systems	272:299	Nevertheless, intermolecular interactions between various neutral and ionic species including large macromolecular systems and simple inorganic ions are the key to understanding the excellent lubrication performance.
34830249	2	43	theme	neutral	236:242	arg1	ions					322:325	simple inorganic ions	305:325	simple inorganic ions	305:325	Nevertheless, intermolecular interactions between various neutral and ionic species including large macromolecular systems and simple inorganic ions are the key to understanding the excellent lubrication performance.
34830249	2	43	theme	neutral	236:242	arg1	species					254:260	various neutral and ionic species	228:260	various neutral and ionic species including large macromolecular systems and simple inorganic ions	228:325	Nevertheless, intermolecular interactions between various neutral and ionic species including large macromolecular systems and simple inorganic ions are the key to understanding the excellent lubrication performance.
34830249	2	43	theme	neutral	236:242	arg1	systems					293:299	large macromolecular systems	272:299	large macromolecular systems	272:299	Nevertheless, intermolecular interactions between various neutral and ionic species including large macromolecular systems and simple inorganic ions are the key to understanding the excellent lubrication performance.
34830249	7	44	theme	molecular	1012:1020	arg1	tools					1031:1035	molecular dynamics tools	1012:1035	molecular dynamics tools	1012:1035	In this study, the influence of Na+, Mg2+, and Ca2+ ions on human serum albumin-hyaluronan interactions were examined using molecular dynamics tools.
34830249	2	45	theme	excellent	360:368	arg1	performance					382:392	the excellent lubrication performance	356:392	the excellent lubrication performance	356:392	Nevertheless, intermolecular interactions between various neutral and ionic species including large macromolecular systems and simple inorganic ions are the key to understanding the excellent lubrication performance.
34830249	1	46	from	mechanism	110:118	arg1	joints					142:147	joints	142:147	joints	142:147	The lubrication mechanism in synovial fluid and joints is not yet fully understood.
34830249	1	46	from	mechanism	110:118	arg1	fluid					132:136	synovial fluid	123:136	synovial fluid	123:136	The lubrication mechanism in synovial fluid and joints is not yet fully understood.
34830249	6	47	theme	solution	858:865	arg1	effects					879:885	solution composition effects	858:885	account solution composition effects	850:885	In the context of synovial fluid, it is relevant to describe the possible interactions between albumin and hyaluronate, taking into account solution composition effects.
34830249	0	48	dep	Influence	33:41	arg1	Interactions					19:30	Albumin-Hyaluronan Interactions	0:30	Albumin-Hyaluronan Interactions	0:30	Albumin-Hyaluronan Interactions: Influence of Ionic Composition Probed by Molecular Dynamics.
34830249	7	49	theme	dynamics	1022:1029	arg1	tools					1031:1035	molecular dynamics tools	1012:1035	molecular dynamics tools	1012:1035	In this study, the influence of Na+, Mg2+, and Ca2+ ions on human serum albumin-hyaluronan interactions were examined using molecular dynamics tools.
34830249	7	50	theme	Mg2+	925:928	arg1	ions					940:943	Na+, Mg2+, and Ca2+ ions	920:943	Na+, Mg2+, and Ca2+ ions	920:943	In this study, the influence of Na+, Mg2+, and Ca2+ ions on human serum albumin-hyaluronan interactions were examined using molecular dynamics tools.
34830249	8	51	from	compensation	1232:1243	arg1	albumin					1282:1288	albumin	1282:1288	albumin	1282:1288	It was established that the presence of divalent cations, and especially Ca2+, contributes mostly to the increase of the affinity between hyaluronan and albumin, which is associated with charge compensation in negatively charged hyaluronan and albumin.
34830249	8	51	from	compensation	1232:1243	arg1	hyaluronan					1267:1276	hyaluronan	1267:1276	hyaluronan	1267:1276	It was established that the presence of divalent cations, and especially Ca2+, contributes mostly to the increase of the affinity between hyaluronan and albumin, which is associated with charge compensation in negatively charged hyaluronan and albumin.
34830249	2	52	theme	macromolecular	278:291	arg1	systems					293:299	large macromolecular systems	272:299	large macromolecular systems	272:299	Nevertheless, intermolecular interactions between various neutral and ionic species including large macromolecular systems and simple inorganic ions are the key to understanding the excellent lubrication performance.
34830249	0	53	theme	Molecular	74:82	arg1	Dynamics					84:91	Molecular Dynamics	74:91	Molecular Dynamics	74:91	Albumin-Hyaluronan Interactions: Influence of Ionic Composition Probed by Molecular Dynamics.
34830249	2	54	theme	intermolecular	192:205	arg1	interactions					207:218	intermolecular interactions	192:218	intermolecular interactions between various neutral and ionic species including large macromolecular systems and simple inorganic ions	192:325	Nevertheless, intermolecular interactions between various neutral and ionic species including large macromolecular systems and simple inorganic ions are the key to understanding the excellent lubrication performance.
34830249	2	54	theme	intermolecular	192:205	arg1	key					335:337	key	335:337	key	335:337	Nevertheless, intermolecular interactions between various neutral and ionic species including large macromolecular systems and simple inorganic ions are the key to understanding the excellent lubrication performance.
34830249	2	55	theme	large	272:276	arg1	systems					293:299	large macromolecular systems	272:299	large macromolecular systems	272:299	Nevertheless, intermolecular interactions between various neutral and ionic species including large macromolecular systems and simple inorganic ions are the key to understanding the excellent lubrication performance.
34830249	10	56	theme	indicated	1391:1399	arg1	moieties					1401:1408	The indicated moieties	1387:1408	The indicated moieties	1387:1408	The indicated moieties exhibit a locally positive charge which enables hyaluronate binding (direct and water mediated).
34830249	7	57	theme	Na+	920:922	arg1	ions					940:943	Na+, Mg2+, and Ca2+ ions	920:943	Na+, Mg2+, and Ca2+ ions	920:943	In this study, the influence of Na+, Mg2+, and Ca2+ ions on human serum albumin-hyaluronan interactions were examined using molecular dynamics tools.
34830249	8	58	theme	charge	1225:1230	arg1	compensation					1232:1243	charge compensation	1225:1243	charge compensation in negatively charged hyaluronan and albumin	1225:1288	It was established that the presence of divalent cations, and especially Ca2+, contributes mostly to the increase of the affinity between hyaluronan and albumin, which is associated with charge compensation in negatively charged hyaluronan and albumin.
34830249	5	59	theme	electrostatic	577:589	arg1	properties					591:600	Its electrostatic properties	573:600	Its electrostatic properties	573:600	Its electrostatic properties, including the ability to form molecular complexes, are closely related to pH, solvation, and the presence of ions.
34830249	5	59	theme	electrostatic	577:589	arg1	ability					617:623	the ability to form molecular complexes	613:651	the ability to form molecular complexes	613:651	Its electrostatic properties, including the ability to form molecular complexes, are closely related to pH, solvation, and the presence of ions.
33811973	8	0	from	metabolism	1123:1132	arg1	gut					1219:1221	gut	1219:1221	gut	1219:1221	M10 had stronger activity than Myricetin in the improvement of biosynthesis and degradation activities, resulting to increasing metabolism of sulfur, pyruvate, steroid biosynthesis and unsaturated fatty acid biosynthesis in gut.
33811973	5	1	theme	16S	576:578	arg1	sequencing					585:594	16S rDNA sequencing	576:594	16S rDNA sequencing assay	576:600	We performed 16S rDNA sequencing assay to analyze the composition of gut microbiota isolated from ileocecum.
33811973	10	2	from	improvements	1340:1351	arg1	UC					1356:1357	UC	1356:1357	UC	1356:1357	It suggests that the improvements in UC are the consequence of the effect of M10 that leads to the changed intestinal microbiota.
33811973	3	3	theme	UC	447:448	arg1	model					438:442	Mice model	433:442	Mice model of UC	433:448	Mice model of UC was induced by dextran sulfate sodium (DSS) treatment.
33811973	1	4	theme	ameliorating	236:247	arg1	UC					269:270	UC	269:270	UC	269:270	Our previous studies found that M10, a myricetin-3-O-β-d-lactose sodium salt, possessed higher effects of ameliorating ulcerative colitis (UC) than Myricetin in mice.
33811973	1	4	theme	ameliorating	236:247	arg1	colitis					260:266	ameliorating ulcerative colitis	236:266	ameliorating ulcerative colitis (UC)	236:271	Our previous studies found that M10, a myricetin-3-O-β-d-lactose sodium salt, possessed higher effects of ameliorating ulcerative colitis (UC) than Myricetin in mice.
33811973	7	5	theme	genus	779:783	arg1	level					785:789	genus level	779:789	genus level	779:789	At genus level, the effects of M10 and Myricetin on colitis were associated to the increase of probiotics, such as Akkermansia, and the inhibition of pathogenic microorganisms, such as Ruminococcus and Parabacteroides.
33811973	5	6	theme	rDNA	580:583	arg1	sequencing					585:594	16S rDNA sequencing	576:594	16S rDNA sequencing assay	576:600	We performed 16S rDNA sequencing assay to analyze the composition of gut microbiota isolated from ileocecum.
33811973	6	7	theme	healthy	758:764	arg1	mice					766:769	healthy mice	758:769	healthy mice had	758:773	Both M10 and Myricetin normalized the composition of Firmicutes and Actinobacteria as healthy mice had.
33811973	6	7	theme	healthy	758:764	arg1	composition					710:720	the composition	706:720	the composition of Firmicutes and Actinobacteria	706:753	Both M10 and Myricetin normalized the composition of Firmicutes and Actinobacteria as healthy mice had.
33811973	8	8	contain	had	999:1001	arg1	M10					995:997	M10	995:997	M10	995:997	M10 had stronger activity than Myricetin in the improvement of biosynthesis and degradation activities, resulting to increasing metabolism of sulfur, pyruvate, steroid biosynthesis and unsaturated fatty acid biosynthesis in gut.
33811973	8	8	contain	had	999:1001	arg2	activity					1012:1019	stronger activity	1003:1019	stronger activity	1003:1019	M10 had stronger activity than Myricetin in the improvement of biosynthesis and degradation activities, resulting to increasing metabolism of sulfur, pyruvate, steroid biosynthesis and unsaturated fatty acid biosynthesis in gut.
33811973	1	9	theme	ulcerative	249:258	arg1	UC					269:270	UC	269:270	UC	269:270	Our previous studies found that M10, a myricetin-3-O-β-d-lactose sodium salt, possessed higher effects of ameliorating ulcerative colitis (UC) than Myricetin in mice.
33811973	1	9	theme	ulcerative	249:258	arg1	colitis					260:266	ameliorating ulcerative colitis	236:266	ameliorating ulcerative colitis (UC)	236:271	Our previous studies found that M10, a myricetin-3-O-β-d-lactose sodium salt, possessed higher effects of ameliorating ulcerative colitis (UC) than Myricetin in mice.
33811973	0	10	theme	microbiota	86:95	arg1	composition					67:77	composition	67:77	composition of gut microbiota in mice with ulcerative colitis	67:127	Myricetin and M10, a myricetin-3-O-β-d-lactose sodium salt, modify composition of gut microbiota in mice with ulcerative colitis.
33811973	5	11	theme	sequencing	585:594	arg1	assay					596:600	16S rDNA sequencing assay	576:600	16S rDNA sequencing assay	576:600	We performed 16S rDNA sequencing assay to analyze the composition of gut microbiota isolated from ileocecum.
33811973	3	12	theme	sodium	481:486	arg1	treatment					494:502	dextran sulfate sodium (DSS) treatment	465:502	dextran sulfate sodium (DSS) treatment	465:502	Mice model of UC was induced by dextran sulfate sodium (DSS) treatment.
33811973	11	13	theme	microbiota	1545:1554	arg1	modification					1514:1525	modification	1514:1525	modification of the intestinal microbiota	1514:1554	Conclusion: M10 contributed the pharmacological effects on UC by modification of the intestinal microbiota.
33811973	1	14	theme	colitis	260:266	arg1	effects					225:231	higher effects	218:231	higher effects of ameliorating ulcerative colitis (UC)	218:271	Our previous studies found that M10, a myricetin-3-O-β-d-lactose sodium salt, possessed higher effects of ameliorating ulcerative colitis (UC) than Myricetin in mice.
33811973	0	15	theme	gut	82:84	arg1	microbiota					86:95	gut microbiota	82:95	gut microbiota	82:95	Myricetin and M10, a myricetin-3-O-β-d-lactose sodium salt, modify composition of gut microbiota in mice with ulcerative colitis.
33811973	6	16	theme	Actinobacteria	740:753	arg1	mice					766:769	healthy mice	758:769	healthy mice had	758:773	Both M10 and Myricetin normalized the composition of Firmicutes and Actinobacteria as healthy mice had.
33811973	6	16	theme	Actinobacteria	740:753	arg1	composition					710:720	the composition	706:720	the composition of Firmicutes and Actinobacteria	706:753	Both M10 and Myricetin normalized the composition of Firmicutes and Actinobacteria as healthy mice had.
33811973	9	17	theme	gut	1303:1305	arg1	microbiota					1307:1316	gut microbiota	1303:1316	gut microbiota	1303:1316	Furthermore, M10 normalized the proportion of Firmicutes and Actinobacteria in gut microbiota.
33811973	8	18	theme	acid	1198:1201	arg1	biosynthesis					1203:1214	unsaturated fatty acid biosynthesis	1180:1214	unsaturated fatty acid biosynthesis in gut	1180:1221	M10 had stronger activity than Myricetin in the improvement of biosynthesis and degradation activities, resulting to increasing metabolism of sulfur, pyruvate, steroid biosynthesis and unsaturated fatty acid biosynthesis in gut.
33811973	8	19	from	pyruvate	1145:1152	arg1	gut					1219:1221	gut	1219:1221	gut	1219:1221	M10 had stronger activity than Myricetin in the improvement of biosynthesis and degradation activities, resulting to increasing metabolism of sulfur, pyruvate, steroid biosynthesis and unsaturated fatty acid biosynthesis in gut.
33811973	7	20	theme	probiotics	871:880	arg1	inhibition					912:921	the inhibition	908:921	the inhibition of pathogenic microorganisms, such as Ruminococcus and Parabacteroides	908:992	At genus level, the effects of M10 and Myricetin on colitis were associated to the increase of probiotics, such as Akkermansia, and the inhibition of pathogenic microorganisms, such as Ruminococcus and Parabacteroides.
33811973	7	20	theme	probiotics	871:880	arg1	increase					859:866	the increase	855:866	the increase of probiotics, such as Akkermansia,	855:902	At genus level, the effects of M10 and Myricetin on colitis were associated to the increase of probiotics, such as Akkermansia, and the inhibition of pathogenic microorganisms, such as Ruminococcus and Parabacteroides.
33811973	3	21	theme	dextran	465:471	arg1	sodium					481:486	dextran sulfate sodium	465:486	dextran sulfate sodium (DSS) treatment	465:502	Mice model of UC was induced by dextran sulfate sodium (DSS) treatment.
33811973	3	21	theme	dextran	465:471	arg1	DSS					489:491	DSS	489:491	DSS	489:491	Mice model of UC was induced by dextran sulfate sodium (DSS) treatment.
33811973	8	22	theme	unsaturated	1180:1190	arg1	biosynthesis					1203:1214	unsaturated fatty acid biosynthesis	1180:1214	unsaturated fatty acid biosynthesis in gut	1180:1221	M10 had stronger activity than Myricetin in the improvement of biosynthesis and degradation activities, resulting to increasing metabolism of sulfur, pyruvate, steroid biosynthesis and unsaturated fatty acid biosynthesis in gut.
33811973	1	23	from	Myricetin	278:286	arg1	mice					291:294	mice	291:294	mice	291:294	Our previous studies found that M10, a myricetin-3-O-β-d-lactose sodium salt, possessed higher effects of ameliorating ulcerative colitis (UC) than Myricetin in mice.
33811973	0	24	with	mice	100:103	arg1	colitis					121:127	ulcerative colitis	110:127	ulcerative colitis	110:127	Myricetin and M10, a myricetin-3-O-β-d-lactose sodium salt, modify composition of gut microbiota in mice with ulcerative colitis.
33811973	3	25	theme	sulfate	473:479	arg1	sodium					481:486	dextran sulfate sodium	465:486	dextran sulfate sodium (DSS) treatment	465:502	Mice model of UC was induced by dextran sulfate sodium (DSS) treatment.
33811973	3	25	theme	sulfate	473:479	arg1	DSS					489:491	DSS	489:491	DSS	489:491	Mice model of UC was induced by dextran sulfate sodium (DSS) treatment.
33811973	5	26	attach	isolated	647:654	arg2	microbiota					636:645	gut microbiota	632:645	gut microbiota isolated from ileocecum	632:669	We performed 16S rDNA sequencing assay to analyze the composition of gut microbiota isolated from ileocecum.
33811973	5	26	attach	isolated	647:654	arg1	ileocecum					661:669	ileocecum	661:669	ileocecum	661:669	We performed 16S rDNA sequencing assay to analyze the composition of gut microbiota isolated from ileocecum.
33811973	8	27	theme	steroid	1155:1161	arg1	biosynthesis					1163:1174	steroid biosynthesis	1155:1174	steroid biosynthesis	1155:1174	M10 had stronger activity than Myricetin in the improvement of biosynthesis and degradation activities, resulting to increasing metabolism of sulfur, pyruvate, steroid biosynthesis and unsaturated fatty acid biosynthesis in gut.
33811973	8	28	theme	biosynthesis	1058:1069	arg1	activities					1087:1096	biosynthesis and degradation activities	1058:1096	biosynthesis and degradation activities	1058:1096	M10 had stronger activity than Myricetin in the improvement of biosynthesis and degradation activities, resulting to increasing metabolism of sulfur, pyruvate, steroid biosynthesis and unsaturated fatty acid biosynthesis in gut.
33811973	2	29	theme	UC	351:352	arg1	consequence					361:371	the consequence	357:371	the consequence of the effects of M10 that leads to the changed microbiota	357:430	Here, we aim to investigate whether the inhibition of UC is the consequence of the effects of M10 that leads to the changed microbiota.
33811973	2	29	theme	UC	351:352	arg1	inhibition					337:346	the inhibition	333:346	the inhibition of UC	333:352	Here, we aim to investigate whether the inhibition of UC is the consequence of the effects of M10 that leads to the changed microbiota.
33811973	9	30	from	proportion	1256:1265	arg1	microbiota					1307:1316	gut microbiota	1303:1316	gut microbiota	1303:1316	Furthermore, M10 normalized the proportion of Firmicutes and Actinobacteria in gut microbiota.
33811973	10	31	theme	effect	1386:1391	arg1	consequence					1367:1377	the consequence	1363:1377	the consequence of the effect of M10 that leads to the changed intestinal microbiota	1363:1446	It suggests that the improvements in UC are the consequence of the effect of M10 that leads to the changed intestinal microbiota.
33811973	10	31	theme	effect	1386:1391	arg1	improvements					1340:1351	the improvements	1336:1351	the improvements in UC	1336:1357	It suggests that the improvements in UC are the consequence of the effect of M10 that leads to the changed intestinal microbiota.
33811973	11	32	theme	pharmacological	1481:1495	arg1	effects					1497:1503	the pharmacological effects	1477:1503	the pharmacological effects on UC	1477:1509	Conclusion: M10 contributed the pharmacological effects on UC by modification of the intestinal microbiota.
33811973	8	33	theme	fatty	1192:1196	arg1	biosynthesis					1203:1214	unsaturated fatty acid biosynthesis	1180:1214	unsaturated fatty acid biosynthesis in gut	1180:1221	M10 had stronger activity than Myricetin in the improvement of biosynthesis and degradation activities, resulting to increasing metabolism of sulfur, pyruvate, steroid biosynthesis and unsaturated fatty acid biosynthesis in gut.
33811973	2	34	theme	M10	391:393	arg1	effects					380:386	the effects	376:386	the effects of M10	376:393	Here, we aim to investigate whether the inhibition of UC is the consequence of the effects of M10 that leads to the changed microbiota.
33811973	9	35	theme	Firmicutes	1270:1279	arg1	proportion					1256:1265	the proportion	1252:1265	the proportion of Firmicutes and Actinobacteria in gut microbiota	1252:1316	Furthermore, M10 normalized the proportion of Firmicutes and Actinobacteria in gut microbiota.
33811973	0	36	theme	ulcerative	110:119	arg1	colitis					121:127	ulcerative colitis	110:127	ulcerative colitis	110:127	Myricetin and M10, a myricetin-3-O-β-d-lactose sodium salt, modify composition of gut microbiota in mice with ulcerative colitis.
33811973	2	37	theme	effects	380:386	arg1	consequence					361:371	the consequence	357:371	the consequence of the effects of M10 that leads to the changed microbiota	357:430	Here, we aim to investigate whether the inhibition of UC is the consequence of the effects of M10 that leads to the changed microbiota.
33811973	2	37	theme	effects	380:386	arg1	inhibition					337:346	the inhibition	333:346	the inhibition of UC	333:352	Here, we aim to investigate whether the inhibition of UC is the consequence of the effects of M10 that leads to the changed microbiota.
33811973	8	38	from	biosynthesis	1203:1214	arg1	gut					1219:1221	gut	1219:1221	gut	1219:1221	M10 had stronger activity than Myricetin in the improvement of biosynthesis and degradation activities, resulting to increasing metabolism of sulfur, pyruvate, steroid biosynthesis and unsaturated fatty acid biosynthesis in gut.
33811973	5	39	theme	gut	632:634	arg1	microbiota					636:645	gut microbiota	632:645	gut microbiota isolated from ileocecum	632:669	We performed 16S rDNA sequencing assay to analyze the composition of gut microbiota isolated from ileocecum.
33811973	8	40	theme	stronger	1003:1010	arg1	activity					1012:1019	stronger activity	1003:1019	stronger activity	1003:1019	M10 had stronger activity than Myricetin in the improvement of biosynthesis and degradation activities, resulting to increasing metabolism of sulfur, pyruvate, steroid biosynthesis and unsaturated fatty acid biosynthesis in gut.
33811973	1	41	theme	sodium	195:200	arg1	salt					202:205	a myricetin-3-O-β-d-lactose sodium salt	167:205	a myricetin-3-O-β-d-lactose sodium salt	167:205	Our previous studies found that M10, a myricetin-3-O-β-d-lactose sodium salt, possessed higher effects of ameliorating ulcerative colitis (UC) than Myricetin in mice.
33811973	1	41	theme	sodium	195:200	arg1	M10					162:164	M10	162:164	M10	162:164	Our previous studies found that M10, a myricetin-3-O-β-d-lactose sodium salt, possessed higher effects of ameliorating ulcerative colitis (UC) than Myricetin in mice.
33811973	0	42	theme	myricetin-3-O-β-d-lactose	21:45	arg1	Myricetin					0:8	Myricetin	0:8	Myricetin	0:8	Myricetin and M10, a myricetin-3-O-β-d-lactose sodium salt, modify composition of gut microbiota in mice with ulcerative colitis.
33811973	0	42	theme	myricetin-3-O-β-d-lactose	21:45	arg1	salt					54:57	a myricetin-3-O-β-d-lactose sodium salt	19:57	a myricetin-3-O-β-d-lactose sodium salt	19:57	Myricetin and M10, a myricetin-3-O-β-d-lactose sodium salt, modify composition of gut microbiota in mice with ulcerative colitis.
33811973	9	43	theme	Actinobacteria	1285:1298	arg1	proportion					1256:1265	the proportion	1252:1265	the proportion of Firmicutes and Actinobacteria in gut microbiota	1252:1316	Furthermore, M10 normalized the proportion of Firmicutes and Actinobacteria in gut microbiota.
33811973	5	44	theme	microbiota	636:645	arg1	composition					617:627	the composition	613:627	the composition of gut microbiota isolated from ileocecum	613:669	We performed 16S rDNA sequencing assay to analyze the composition of gut microbiota isolated from ileocecum.
33811973	0	45	from	composition	67:77	arg1	mice					100:103	mice	100:103	mice with ulcerative colitis	100:127	Myricetin and M10, a myricetin-3-O-β-d-lactose sodium salt, modify composition of gut microbiota in mice with ulcerative colitis.
33811973	2	46	theme	changed	413:419	arg1	microbiota					421:430	the changed microbiota	409:430	the changed microbiota	409:430	Here, we aim to investigate whether the inhibition of UC is the consequence of the effects of M10 that leads to the changed microbiota.
33811973	8	47	theme	sulfur	1137:1142	arg1	metabolism					1123:1132	metabolism	1123:1132	metabolism of sulfur	1123:1142	M10 had stronger activity than Myricetin in the improvement of biosynthesis and degradation activities, resulting to increasing metabolism of sulfur, pyruvate, steroid biosynthesis and unsaturated fatty acid biosynthesis in gut.
33811973	8	47	theme	sulfur	1137:1142	arg1	pyruvate					1145:1152	pyruvate	1145:1152	pyruvate	1145:1152	M10 had stronger activity than Myricetin in the improvement of biosynthesis and degradation activities, resulting to increasing metabolism of sulfur, pyruvate, steroid biosynthesis and unsaturated fatty acid biosynthesis in gut.
33811973	8	47	theme	sulfur	1137:1142	arg1	biosynthesis					1203:1214	unsaturated fatty acid biosynthesis	1180:1214	unsaturated fatty acid biosynthesis in gut	1180:1221	M10 had stronger activity than Myricetin in the improvement of biosynthesis and degradation activities, resulting to increasing metabolism of sulfur, pyruvate, steroid biosynthesis and unsaturated fatty acid biosynthesis in gut.
33811973	8	47	theme	sulfur	1137:1142	arg1	biosynthesis					1163:1174	steroid biosynthesis	1155:1174	steroid biosynthesis	1155:1174	M10 had stronger activity than Myricetin in the improvement of biosynthesis and degradation activities, resulting to increasing metabolism of sulfur, pyruvate, steroid biosynthesis and unsaturated fatty acid biosynthesis in gut.
33811973	10	48	theme	M10	1396:1398	arg1	effect					1386:1391	the effect	1382:1391	the effect of M10	1382:1398	It suggests that the improvements in UC are the consequence of the effect of M10 that leads to the changed intestinal microbiota.
33811973	8	49	theme	activities	1087:1096	arg1	improvement					1043:1053	the improvement	1039:1053	the improvement of biosynthesis and degradation activities	1039:1096	M10 had stronger activity than Myricetin in the improvement of biosynthesis and degradation activities, resulting to increasing metabolism of sulfur, pyruvate, steroid biosynthesis and unsaturated fatty acid biosynthesis in gut.
33811973	7	50	theme	pathogenic	926:935	arg1	microorganisms					937:950	pathogenic microorganisms	926:950	pathogenic microorganisms	926:950	At genus level, the effects of M10 and Myricetin on colitis were associated to the increase of probiotics, such as Akkermansia, and the inhibition of pathogenic microorganisms, such as Ruminococcus and Parabacteroides.
33811973	7	50	theme	pathogenic	926:935	arg1	Parabacteroides					978:992	Parabacteroides	978:992	Parabacteroides	978:992	At genus level, the effects of M10 and Myricetin on colitis were associated to the increase of probiotics, such as Akkermansia, and the inhibition of pathogenic microorganisms, such as Ruminococcus and Parabacteroides.
33811973	7	50	theme	pathogenic	926:935	arg1	Ruminococcus					961:972	Ruminococcus	961:972	Ruminococcus	961:972	At genus level, the effects of M10 and Myricetin on colitis were associated to the increase of probiotics, such as Akkermansia, and the inhibition of pathogenic microorganisms, such as Ruminococcus and Parabacteroides.
33811973	0	51	theme	sodium	47:52	arg1	Myricetin					0:8	Myricetin	0:8	Myricetin	0:8	Myricetin and M10, a myricetin-3-O-β-d-lactose sodium salt, modify composition of gut microbiota in mice with ulcerative colitis.
33811973	0	51	theme	sodium	47:52	arg1	salt					54:57	a myricetin-3-O-β-d-lactose sodium salt	19:57	a myricetin-3-O-β-d-lactose sodium salt	19:57	Myricetin and M10, a myricetin-3-O-β-d-lactose sodium salt, modify composition of gut microbiota in mice with ulcerative colitis.
33811973	10	52	theme	changed	1418:1424	arg1	microbiota					1437:1446	the changed intestinal microbiota	1414:1446	the changed intestinal microbiota	1414:1446	It suggests that the improvements in UC are the consequence of the effect of M10 that leads to the changed intestinal microbiota.
33811973	1	53	contain	possessed	208:216	arg1	salt					202:205	a myricetin-3-O-β-d-lactose sodium salt	167:205	a myricetin-3-O-β-d-lactose sodium salt	167:205	Our previous studies found that M10, a myricetin-3-O-β-d-lactose sodium salt, possessed higher effects of ameliorating ulcerative colitis (UC) than Myricetin in mice.
33811973	1	53	contain	possessed	208:216	arg1	M10					162:164	M10	162:164	M10	162:164	Our previous studies found that M10, a myricetin-3-O-β-d-lactose sodium salt, possessed higher effects of ameliorating ulcerative colitis (UC) than Myricetin in mice.
33811973	1	53	contain	possessed	208:216	arg2	effects					225:231	higher effects	218:231	higher effects of ameliorating ulcerative colitis (UC)	218:271	Our previous studies found that M10, a myricetin-3-O-β-d-lactose sodium salt, possessed higher effects of ameliorating ulcerative colitis (UC) than Myricetin in mice.
33811973	8	54	from	biosynthesis	1163:1174	arg1	gut					1219:1221	gut	1219:1221	gut	1219:1221	M10 had stronger activity than Myricetin in the improvement of biosynthesis and degradation activities, resulting to increasing metabolism of sulfur, pyruvate, steroid biosynthesis and unsaturated fatty acid biosynthesis in gut.
33811973	11	55	from	effects	1497:1503	arg1	UC					1508:1509	UC	1508:1509	UC	1508:1509	Conclusion: M10 contributed the pharmacological effects on UC by modification of the intestinal microbiota.
33811973	7	56	theme	microorganisms	937:950	arg1	inhibition					912:921	the inhibition	908:921	the inhibition of pathogenic microorganisms, such as Ruminococcus and Parabacteroides	908:992	At genus level, the effects of M10 and Myricetin on colitis were associated to the increase of probiotics, such as Akkermansia, and the inhibition of pathogenic microorganisms, such as Ruminococcus and Parabacteroides.
33811973	7	56	theme	microorganisms	937:950	arg1	increase					859:866	the increase	855:866	the increase of probiotics, such as Akkermansia,	855:902	At genus level, the effects of M10 and Myricetin on colitis were associated to the increase of probiotics, such as Akkermansia, and the inhibition of pathogenic microorganisms, such as Ruminococcus and Parabacteroides.
33811973	6	57	theme	Firmicutes	725:734	arg1	mice					766:769	healthy mice	758:769	healthy mice had	758:773	Both M10 and Myricetin normalized the composition of Firmicutes and Actinobacteria as healthy mice had.
33811973	6	57	theme	Firmicutes	725:734	arg1	composition					710:720	the composition	706:720	the composition of Firmicutes and Actinobacteria	706:753	Both M10 and Myricetin normalized the composition of Firmicutes and Actinobacteria as healthy mice had.
33811973	10	58	theme	intestinal	1426:1435	arg1	microbiota					1437:1446	the changed intestinal microbiota	1414:1446	the changed intestinal microbiota	1414:1446	It suggests that the improvements in UC are the consequence of the effect of M10 that leads to the changed intestinal microbiota.
33811973	7	59	from	effects	796:802	arg1	colitis					828:834	colitis	828:834	colitis	828:834	At genus level, the effects of M10 and Myricetin on colitis were associated to the increase of probiotics, such as Akkermansia, and the inhibition of pathogenic microorganisms, such as Ruminococcus and Parabacteroides.
33811973	3	60	theme	Mice	433:436	arg1	model					438:442	Mice model	433:442	Mice model of UC	433:448	Mice model of UC was induced by dextran sulfate sodium (DSS) treatment.
33811973	7	61	theme	Myricetin	815:823	arg1	effects					796:802	the effects	792:802	the effects of M10 and Myricetin on colitis	792:834	At genus level, the effects of M10 and Myricetin on colitis were associated to the increase of probiotics, such as Akkermansia, and the inhibition of pathogenic microorganisms, such as Ruminococcus and Parabacteroides.
33811973	1	62	theme	higher	218:223	arg1	effects					225:231	higher effects	218:231	higher effects of ameliorating ulcerative colitis (UC)	218:271	Our previous studies found that M10, a myricetin-3-O-β-d-lactose sodium salt, possessed higher effects of ameliorating ulcerative colitis (UC) than Myricetin in mice.
33811973	1	63	theme	myricetin-3-O-β-d-lactose	169:193	arg1	salt					202:205	a myricetin-3-O-β-d-lactose sodium salt	167:205	a myricetin-3-O-β-d-lactose sodium salt	167:205	Our previous studies found that M10, a myricetin-3-O-β-d-lactose sodium salt, possessed higher effects of ameliorating ulcerative colitis (UC) than Myricetin in mice.
33811973	1	63	theme	myricetin-3-O-β-d-lactose	169:193	arg1	M10					162:164	M10	162:164	M10	162:164	Our previous studies found that M10, a myricetin-3-O-β-d-lactose sodium salt, possessed higher effects of ameliorating ulcerative colitis (UC) than Myricetin in mice.
33811973	8	64	from	Myricetin	1026:1034	arg1	improvement					1043:1053	the improvement	1039:1053	the improvement of biosynthesis and degradation activities	1039:1096	M10 had stronger activity than Myricetin in the improvement of biosynthesis and degradation activities, resulting to increasing metabolism of sulfur, pyruvate, steroid biosynthesis and unsaturated fatty acid biosynthesis in gut.
33811973	11	65	theme	intestinal	1534:1543	arg1	microbiota					1545:1554	the intestinal microbiota	1530:1554	the intestinal microbiota	1530:1554	Conclusion: M10 contributed the pharmacological effects on UC by modification of the intestinal microbiota.
33811973	1	66	theme	previous	134:141	arg1	studies					143:149	Our previous studies	130:149	Our previous studies	130:149	Our previous studies found that M10, a myricetin-3-O-β-d-lactose sodium salt, possessed higher effects of ameliorating ulcerative colitis (UC) than Myricetin in mice.
33811973	7	67	theme	M10	807:809	arg1	effects					796:802	the effects	792:802	the effects of M10 and Myricetin on colitis	792:834	At genus level, the effects of M10 and Myricetin on colitis were associated to the increase of probiotics, such as Akkermansia, and the inhibition of pathogenic microorganisms, such as Ruminococcus and Parabacteroides.
33811973	8	68	theme	degradation	1075:1085	arg1	activities					1087:1096	biosynthesis and degradation activities	1058:1096	biosynthesis and degradation activities	1058:1096	M10 had stronger activity than Myricetin in the improvement of biosynthesis and degradation activities, resulting to increasing metabolism of sulfur, pyruvate, steroid biosynthesis and unsaturated fatty acid biosynthesis in gut.
33909078	6	0	theme	High-performance	986:1001	arg1	HPLC					1026:1029	HPLC	1026:1029	HPLC	1026:1029	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	6	0	theme	High-performance	986:1001	arg1	chromatography					1010:1023	High-performance liquid chromatography	986:1023	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides	986:1084	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	7	1	theme	GT	1339:1340	arg1	Gmh1p					1381:1385	Gmh1p	1381:1385	Gmh1p	1381:1385	Moreover, 1H-NMR of N-glycans revealed that three GT family 34 α1,2-galactosyltransferases (Gmh1p, Gmh2p and Gmh3p) are required for the galactosylation of N-linked oligosaccharides.
33909078	7	1	theme	GT	1339:1340	arg1	α1,2-galactosyltransferases					1352:1378	three GT family 34 α1,2-galactosyltransferases	1333:1378	three GT family 34 α1,2-galactosyltransferases (Gmh1p, Gmh2p and Gmh3p)	1333:1403	Moreover, 1H-NMR of N-glycans revealed that three GT family 34 α1,2-galactosyltransferases (Gmh1p, Gmh2p and Gmh3p) are required for the galactosylation of N-linked oligosaccharides.
33909078	7	1	theme	GT	1339:1340	arg1	Gmh3p					1398:1402	Gmh3p	1398:1402	Gmh3p	1398:1402	Moreover, 1H-NMR of N-glycans revealed that three GT family 34 α1,2-galactosyltransferases (Gmh1p, Gmh2p and Gmh3p) are required for the galactosylation of N-linked oligosaccharides.
33909078	7	1	theme	GT	1339:1340	arg1	Gmh2p					1388:1392	Gmh2p	1388:1392	Gmh2p	1388:1392	Moreover, 1H-NMR of N-glycans revealed that three GT family 34 α1,2-galactosyltransferases (Gmh1p, Gmh2p and Gmh3p) are required for the galactosylation of N-linked oligosaccharides.
33909078	9	2	with	activity	1798:1805	arg1	specificities					1844:1856	different substrate specificities	1824:1856	different substrate specificities	1824:1856	In conclusion, eight galactosyltransferases have been shown to have activity in S. pombe with different substrate specificities.
33909078	8	3	from	activity	1699:1706	arg1	pombe					1723:1727	S. pombe	1720:1727	S. pombe	1720:1727	Furthermore, HPLC and lectin-blot analysis revealed that Otg1p showed α1,3-galactosyltransferase activity under conditions of co-expression with Gmh6p, indicating that α-1,2-linked galactose is required for the galactosylation activity of Otg1p in S. pombe.
33909078	1	4	link	O-linked	258:265	arg1	oligosaccharides					267:282	O-linked oligosaccharides	258:282	O-linked oligosaccharides	258:282	In the fission yeast Schizosaccharomyces pombe, α1,2- and α1,3-linked D-galactose (Gal) residues are transferred to N- and O-linked oligosaccharides of glycoproteins by galactosyltransferases.
33909078	3	5	theme	GT	621:622	arg1	family					625:630	glycosyltransferase (GT) family 34	600:633	glycosyltransferase (GT) family 34	600:633	Schizosaccharomyces pombe has 10 galactosyltransferase-related genes: seven belonging to glycosyltransferase (GT) family 34 and three belonging GT family 8.
33909078	1	6	theme	glycoproteins	287:299	arg1	N-					251:252	N-	251:252	N-	251:252	In the fission yeast Schizosaccharomyces pombe, α1,2- and α1,3-linked D-galactose (Gal) residues are transferred to N- and O-linked oligosaccharides of glycoproteins by galactosyltransferases.
33909078	1	6	theme	glycoproteins	287:299	arg1	oligosaccharides					267:282	O-linked oligosaccharides	258:282	O-linked oligosaccharides	258:282	In the fission yeast Schizosaccharomyces pombe, α1,2- and α1,3-linked D-galactose (Gal) residues are transferred to N- and O-linked oligosaccharides of glycoproteins by galactosyltransferases.
33909078	5	7	dep	function	825:832	arg1	the					821:823	the	821:823	the	821:823	Here, we have investigated the function and substrate specificities of galactosyltransferases in S pombe by using strains expressing single α-galactosyltransferases in the Δ10GalT background.
33909078	2	8	from	communication	384:396	arg1	pombe					404:408	S. pombe	401:408	S. pombe	401:408	Although the galactomannans are important for cell-cell communication in S. pombe (e.g., in nonsexual aggregation), the mechanisms underlying galactosylation in cells remain unclear.
33909078	6	9	theme	O-linked	1263:1270	arg1	oligosaccharide					1272:1286	O-linked oligosaccharide	1263:1286	O-linked oligosaccharide	1263:1286	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	3	10	theme	glycosyltransferase	600:618	arg1	family					625:630	glycosyltransferase (GT) family 34	600:633	glycosyltransferase (GT) family 34	600:633	Schizosaccharomyces pombe has 10 galactosyltransferase-related genes: seven belonging to glycosyltransferase (GT) family 34 and three belonging GT family 8.
33909078	1	11	theme	α1,3-linked	193:203	arg1	residues					223:230	α1,3-linked D-galactose (Gal) residues	193:230	α1,3-linked D-galactose (Gal) residues	193:230	In the fission yeast Schizosaccharomyces pombe, α1,2- and α1,3-linked D-galactose (Gal) residues are transferred to N- and O-linked oligosaccharides of glycoproteins by galactosyltransferases.
33909078	9	12	theme	different	1824:1832	arg1	specificities					1844:1856	different substrate specificities	1824:1856	different substrate specificities	1824:1856	In conclusion, eight galactosyltransferases have been shown to have activity in S. pombe with different substrate specificities.
33909078	10	13	theme	N-	1943:1944	arg1	galactosylation					1919:1933	the galactosylation	1915:1933	the galactosylation of both N- and O-glycans in fission yeast	1915:1975	These findings will be useful for genetically tailoring the galactosylation of both N- and O-glycans in fission yeast.
33909078	5	14	theme	single	927:932	arg1	α-galactosyltransferases					934:957	single α-galactosyltransferases	927:957	single α-galactosyltransferases	927:957	Here, we have investigated the function and substrate specificities of galactosyltransferases in S pombe by using strains expressing single α-galactosyltransferases in the Δ10GalT background.
33909078	6	15	theme	pyridylaminated	1044:1058	arg1	oligosaccharides					1069:1084	pyridylaminated O-linked oligosaccharides	1044:1084	pyridylaminated O-linked oligosaccharides	1044:1084	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	7	16	theme	family	1342:1347	arg1	Gmh1p					1381:1385	Gmh1p	1381:1385	Gmh1p	1381:1385	Moreover, 1H-NMR of N-glycans revealed that three GT family 34 α1,2-galactosyltransferases (Gmh1p, Gmh2p and Gmh3p) are required for the galactosylation of N-linked oligosaccharides.
33909078	7	16	theme	family	1342:1347	arg1	α1,2-galactosyltransferases					1352:1378	three GT family 34 α1,2-galactosyltransferases	1333:1378	three GT family 34 α1,2-galactosyltransferases (Gmh1p, Gmh2p and Gmh3p)	1333:1403	Moreover, 1H-NMR of N-glycans revealed that three GT family 34 α1,2-galactosyltransferases (Gmh1p, Gmh2p and Gmh3p) are required for the galactosylation of N-linked oligosaccharides.
33909078	7	16	theme	family	1342:1347	arg1	Gmh3p					1398:1402	Gmh3p	1398:1402	Gmh3p	1398:1402	Moreover, 1H-NMR of N-glycans revealed that three GT family 34 α1,2-galactosyltransferases (Gmh1p, Gmh2p and Gmh3p) are required for the galactosylation of N-linked oligosaccharides.
33909078	7	16	theme	family	1342:1347	arg1	Gmh2p					1388:1392	Gmh2p	1388:1392	Gmh2p	1388:1392	Moreover, 1H-NMR of N-glycans revealed that three GT family 34 α1,2-galactosyltransferases (Gmh1p, Gmh2p and Gmh3p) are required for the galactosylation of N-linked oligosaccharides.
33909078	10	17	theme	fission	1963:1969	arg1	yeast					1971:1975	fission yeast	1963:1975	fission yeast	1963:1975	These findings will be useful for genetically tailoring the galactosylation of both N- and O-glycans in fission yeast.
33909078	9	18	from	activity	1798:1805	arg1	pombe					1813:1817	S. pombe	1810:1817	S. pombe	1810:1817	In conclusion, eight galactosyltransferases have been shown to have activity in S. pombe with different substrate specificities.
33909078	10	19	theme	O-glycans	1950:1958	arg1	galactosylation					1919:1933	the galactosylation	1915:1933	the galactosylation of both N- and O-glycans in fission yeast	1915:1975	These findings will be useful for genetically tailoring the galactosylation of both N- and O-glycans in fission yeast.
33909078	6	20	theme	chromatography	1010:1023	arg1	analysis					1032:1039	High-performance liquid chromatography (HPLC) analysis	986:1039	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides	986:1084	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	0	21	from	pombe	128:132	arg1	specificities					10:22	Substrate specificities	0:22	Substrate specificities of α1,2- and α1,3-galactosyltransferases and characterization of Gmh1p and Otg1p in Schizosaccharomyces pombe.	0:133	Substrate specificities of α1,2- and α1,3-galactosyltransferases and characterization of Gmh1p and Otg1p in Schizosaccharomyces pombe.
33909078	7	22	theme	oligosaccharides	1454:1469	arg1	galactosylation					1426:1440	the galactosylation	1422:1440	the galactosylation of N-linked oligosaccharides	1422:1469	Moreover, 1H-NMR of N-glycans revealed that three GT family 34 α1,2-galactosyltransferases (Gmh1p, Gmh2p and Gmh3p) are required for the galactosylation of N-linked oligosaccharides.
33909078	2	23	dep	important	360:368	arg1	galactomannans					341:354	the galactomannans	337:354	the galactomannans	337:354	Although the galactomannans are important for cell-cell communication in S. pombe (e.g., in nonsexual aggregation), the mechanisms underlying galactosylation in cells remain unclear.
33909078	2	23	dep	important	360:368	arg1	important					360:368	important	360:368	important	360:368	Although the galactomannans are important for cell-cell communication in S. pombe (e.g., in nonsexual aggregation), the mechanisms underlying galactosylation in cells remain unclear.
33909078	2	23	dep	important	360:368	arg1	e.g.					411:414	e.g.	411:414	e.g.	411:414	Although the galactomannans are important for cell-cell communication in S. pombe (e.g., in nonsexual aggregation), the mechanisms underlying galactosylation in cells remain unclear.
33909078	6	24	link	O-linked	1263:1270	arg1	oligosaccharide					1272:1286	O-linked oligosaccharide	1263:1286	O-linked oligosaccharide	1263:1286	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	10	25	from	galactosylation	1919:1933	arg1	yeast					1971:1975	fission yeast	1963:1975	fission yeast	1963:1975	These findings will be useful for genetically tailoring the galactosylation of both N- and O-glycans in fission yeast.
33909078	6	26	theme	34	1112:1113	arg1	Gmh6p					1155:1159	Gmh6p	1155:1159	Gmh6p	1155:1159	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	6	26	theme	34	1112:1113	arg1	Gma12p					1144:1149	Gma12p	1144:1149	Gma12p	1144:1149	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	6	26	theme	34	1112:1113	arg1	α1,2-galactosyltransferases					1115:1141	two GT family 34 α1,2-galactosyltransferases	1098:1141	two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p)	1098:1160	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	5	27	from	function	825:832	arg1	S					891:891	S	891:891	S	891:891	Here, we have investigated the function and substrate specificities of galactosyltransferases in S pombe by using strains expressing single α-galactosyltransferases in the Δ10GalT background.
33909078	6	28	theme	GT	1102:1103	arg1	Gmh6p					1155:1159	Gmh6p	1155:1159	Gmh6p	1155:1159	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	6	28	theme	GT	1102:1103	arg1	Gma12p					1144:1149	Gma12p	1144:1149	Gma12p	1144:1149	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	6	28	theme	GT	1102:1103	arg1	α1,2-galactosyltransferases					1115:1141	two GT family 34 α1,2-galactosyltransferases	1098:1141	two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p)	1098:1160	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	5	29	theme	Δ10GalT	966:972	arg1	background					974:983	the Δ10GalT background	962:983	the Δ10GalT background	962:983	Here, we have investigated the function and substrate specificities of galactosyltransferases in S pombe by using strains expressing single α-galactosyltransferases in the Δ10GalT background.
33909078	4	30	theme	residues	784:791	arg1	lack					770:773	a complete lack	759:773	a complete lack of α-Gal residues	759:791	Disruption of all 10 α-galactosyltransferases (strain Δ10GalT) has been shown to result in a complete lack of α-Gal residues.
33909078	6	31	theme	oligosaccharides	1069:1084	arg1	analysis					1032:1039	High-performance liquid chromatography (HPLC) analysis	986:1039	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides	986:1084	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	4	32	theme	α-Gal	778:782	arg1	residues					784:791	α-Gal residues	778:791	α-Gal residues	778:791	Disruption of all 10 α-galactosyltransferases (strain Δ10GalT) has been shown to result in a complete lack of α-Gal residues.
33909078	0	33	theme	Substrate	0:8	arg1	specificities					10:22	Substrate specificities	0:22	Substrate specificities of α1,2- and α1,3-galactosyltransferases and characterization of Gmh1p and Otg1p in Schizosaccharomyces pombe.	0:133	Substrate specificities of α1,2- and α1,3-galactosyltransferases and characterization of Gmh1p and Otg1p in Schizosaccharomyces pombe.
33909078	1	34	gly	glycoproteins	287:299	arg1	glycoproteins					287:299	glycoproteins	287:299	glycoproteins	287:299	In the fission yeast Schizosaccharomyces pombe, α1,2- and α1,3-linked D-galactose (Gal) residues are transferred to N- and O-linked oligosaccharides of glycoproteins by galactosyltransferases.
33909078	5	35	theme	galactosyltransferases	865:886	arg1	function					825:832	function	825:832	function	825:832	Here, we have investigated the function and substrate specificities of galactosyltransferases in S pombe by using strains expressing single α-galactosyltransferases in the Δ10GalT background.
33909078	5	35	theme	galactosyltransferases	865:886	arg1	specificities					848:860	substrate specificities	838:860	substrate specificities	838:860	Here, we have investigated the function and substrate specificities of galactosyltransferases in S pombe by using strains expressing single α-galactosyltransferases in the Δ10GalT background.
33909078	7	36	dep	α1,2-galactosyltransferases	1352:1378	arg1	Gmh1p					1381:1385	Gmh1p	1381:1385	Gmh1p	1381:1385	Moreover, 1H-NMR of N-glycans revealed that three GT family 34 α1,2-galactosyltransferases (Gmh1p, Gmh2p and Gmh3p) are required for the galactosylation of N-linked oligosaccharides.
33909078	7	36	dep	α1,2-galactosyltransferases	1352:1378	arg1	α1,2-galactosyltransferases					1352:1378	three GT family 34 α1,2-galactosyltransferases	1333:1378	three GT family 34 α1,2-galactosyltransferases (Gmh1p, Gmh2p and Gmh3p)	1333:1403	Moreover, 1H-NMR of N-glycans revealed that three GT family 34 α1,2-galactosyltransferases (Gmh1p, Gmh2p and Gmh3p) are required for the galactosylation of N-linked oligosaccharides.
33909078	7	36	dep	α1,2-galactosyltransferases	1352:1378	arg1	Gmh3p					1398:1402	Gmh3p	1398:1402	Gmh3p	1398:1402	Moreover, 1H-NMR of N-glycans revealed that three GT family 34 α1,2-galactosyltransferases (Gmh1p, Gmh2p and Gmh3p) are required for the galactosylation of N-linked oligosaccharides.
33909078	7	36	dep	α1,2-galactosyltransferases	1352:1378	arg1	Gmh2p					1388:1392	Gmh2p	1388:1392	Gmh2p	1388:1392	Moreover, 1H-NMR of N-glycans revealed that three GT family 34 α1,2-galactosyltransferases (Gmh1p, Gmh2p and Gmh3p) are required for the galactosylation of N-linked oligosaccharides.
33909078	8	37	theme	Otg1p	1711:1715	arg1	activity					1699:1706	the galactosylation activity	1679:1706	the galactosylation activity of Otg1p in S. pombe	1679:1727	Furthermore, HPLC and lectin-blot analysis revealed that Otg1p showed α1,3-galactosyltransferase activity under conditions of co-expression with Gmh6p, indicating that α-1,2-linked galactose is required for the galactosylation activity of Otg1p in S. pombe.
33909078	8	38	with	co-expression	1598:1610	arg1	Gmh6p					1617:1621	Gmh6p	1617:1621	Gmh6p	1617:1621	Furthermore, HPLC and lectin-blot analysis revealed that Otg1p showed α1,3-galactosyltransferase activity under conditions of co-expression with Gmh6p, indicating that α-1,2-linked galactose is required for the galactosylation activity of Otg1p in S. pombe.
33909078	8	39	theme	galactosylation	1683:1697	arg1	activity					1699:1706	the galactosylation activity	1679:1706	the galactosylation activity of Otg1p in S. pombe	1679:1727	Furthermore, HPLC and lectin-blot analysis revealed that Otg1p showed α1,3-galactosyltransferase activity under conditions of co-expression with Gmh6p, indicating that α-1,2-linked galactose is required for the galactosylation activity of Otg1p in S. pombe.
33909078	0	40	from	α1,2-	27:31	arg1	pombe					128:132	Schizosaccharomyces pombe	108:132	Schizosaccharomyces pombe	108:132	Substrate specificities of α1,2- and α1,3-galactosyltransferases and characterization of Gmh1p and Otg1p in Schizosaccharomyces pombe.
33909078	2	41	theme	cell-cell	374:382	arg1	communication					384:396	cell-cell communication	374:396	cell-cell communication in S. pombe	374:408	Although the galactomannans are important for cell-cell communication in S. pombe (e.g., in nonsexual aggregation), the mechanisms underlying galactosylation in cells remain unclear.
33909078	6	42	theme	liquid	1003:1008	arg1	HPLC					1026:1029	HPLC	1026:1029	HPLC	1026:1029	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	6	42	theme	liquid	1003:1008	arg1	chromatography					1010:1023	High-performance liquid chromatography	986:1023	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides	986:1084	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	7	43	theme	N-glycans	1309:1317	arg1	1H-NMR					1299:1304	1H-NMR	1299:1304	1H-NMR of N-glycans	1299:1317	Moreover, 1H-NMR of N-glycans revealed that three GT family 34 α1,2-galactosyltransferases (Gmh1p, Gmh2p and Gmh3p) are required for the galactosylation of N-linked oligosaccharides.
33909078	1	44	theme	O-linked	258:265	arg1	oligosaccharides					267:282	O-linked oligosaccharides	258:282	O-linked oligosaccharides	258:282	In the fission yeast Schizosaccharomyces pombe, α1,2- and α1,3-linked D-galactose (Gal) residues are transferred to N- and O-linked oligosaccharides of glycoproteins by galactosyltransferases.
33909078	6	45	theme	family	1173:1178	arg1	α1,3-galactosyltransferases					1182:1208	two GT family 8 α1,3-galactosyltransferases	1166:1208	two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p)	1166:1226	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	6	45	theme	family	1173:1178	arg1	Otg3p					1221:1225	Otg3p	1221:1225	Otg3p	1221:1225	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	6	45	theme	family	1173:1178	arg1	Otg2p					1211:1215	Otg2p	1211:1215	Otg2p	1211:1215	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	6	46	theme	oligosaccharide	1272:1286	arg1	galactosylation					1244:1258	galactosylation	1244:1258	galactosylation of O-linked oligosaccharide	1244:1286	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	4	47	theme	strain	715:720	arg1	α-galactosyltransferases					689:712	all 10 α-galactosyltransferases	682:712	all 10 α-galactosyltransferases (strain Δ10GalT)	682:729	Disruption of all 10 α-galactosyltransferases (strain Δ10GalT) has been shown to result in a complete lack of α-Gal residues.
33909078	4	47	theme	strain	715:720	arg1	Δ10GalT					722:728	strain Δ10GalT	715:728	strain Δ10GalT	715:728	Disruption of all 10 α-galactosyltransferases (strain Δ10GalT) has been shown to result in a complete lack of α-Gal residues.
33909078	1	48	theme	D-galactose	205:215	arg1	residues					223:230	α1,3-linked D-galactose (Gal) residues	193:230	α1,3-linked D-galactose (Gal) residues	193:230	In the fission yeast Schizosaccharomyces pombe, α1,2- and α1,3-linked D-galactose (Gal) residues are transferred to N- and O-linked oligosaccharides of glycoproteins by galactosyltransferases.
33909078	6	49	theme	O-linked	1060:1067	arg1	oligosaccharides					1069:1084	pyridylaminated O-linked oligosaccharides	1044:1084	pyridylaminated O-linked oligosaccharides	1044:1084	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	9	50	theme	substrate	1834:1842	arg1	specificities					1844:1856	different substrate specificities	1824:1856	different substrate specificities	1824:1856	In conclusion, eight galactosyltransferases have been shown to have activity in S. pombe with different substrate specificities.
33909078	1	51	theme	Gal	218:220	arg1	residues					223:230	α1,3-linked D-galactose (Gal) residues	193:230	α1,3-linked D-galactose (Gal) residues	193:230	In the fission yeast Schizosaccharomyces pombe, α1,2- and α1,3-linked D-galactose (Gal) residues are transferred to N- and O-linked oligosaccharides of glycoproteins by galactosyltransferases.
33909078	3	52	theme	GT	655:656	arg1	family					658:663	GT family 8	655:665	GT family 8	655:665	Schizosaccharomyces pombe has 10 galactosyltransferase-related genes: seven belonging to glycosyltransferase (GT) family 34 and three belonging GT family 8.
33909078	8	53	theme	co-expression	1598:1610	arg1	conditions					1584:1593	conditions	1584:1593	conditions of co-expression with Gmh6p	1584:1621	Furthermore, HPLC and lectin-blot analysis revealed that Otg1p showed α1,3-galactosyltransferase activity under conditions of co-expression with Gmh6p, indicating that α-1,2-linked galactose is required for the galactosylation activity of Otg1p in S. pombe.
33909078	4	54	theme	α-galactosyltransferases	689:712	arg1	Disruption					668:677	Disruption	668:677	Disruption of all 10 α-galactosyltransferases (strain Δ10GalT)	668:729	Disruption of all 10 α-galactosyltransferases (strain Δ10GalT) has been shown to result in a complete lack of α-Gal residues.
33909078	6	55	link	O-linked	1060:1067	arg1	oligosaccharides					1069:1084	pyridylaminated O-linked oligosaccharides	1044:1084	pyridylaminated O-linked oligosaccharides	1044:1084	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	1	56	theme	fission	142:148	arg1	yeast					150:154	fission yeast	142:154	the fission yeast Schizosaccharomyces pombe	138:180	In the fission yeast Schizosaccharomyces pombe, α1,2- and α1,3-linked D-galactose (Gal) residues are transferred to N- and O-linked oligosaccharides of glycoproteins by galactosyltransferases.
33909078	4	57	theme	complete	761:768	arg1	lack					770:773	a complete lack	759:773	a complete lack of α-Gal residues	759:791	Disruption of all 10 α-galactosyltransferases (strain Δ10GalT) has been shown to result in a complete lack of α-Gal residues.
33909078	0	58	theme	Gmh1p	89:93	arg1	α1,3-galactosyltransferases					37:63	α1,3-galactosyltransferases	37:63	α1,3-galactosyltransferases	37:63	Substrate specificities of α1,2- and α1,3-galactosyltransferases and characterization of Gmh1p and Otg1p in Schizosaccharomyces pombe.
33909078	0	58	theme	Gmh1p	89:93	arg1	α1,2-					27:31	α1,2-	27:31	α1,2-	27:31	Substrate specificities of α1,2- and α1,3-galactosyltransferases and characterization of Gmh1p and Otg1p in Schizosaccharomyces pombe.
33909078	0	58	theme	Gmh1p	89:93	arg1	characterization					69:84	characterization	69:84	characterization	69:84	Substrate specificities of α1,2- and α1,3-galactosyltransferases and characterization of Gmh1p and Otg1p in Schizosaccharomyces pombe.
33909078	6	59	theme	family	1105:1110	arg1	Gmh6p					1155:1159	Gmh6p	1155:1159	Gmh6p	1155:1159	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	6	59	theme	family	1105:1110	arg1	Gma12p					1144:1149	Gma12p	1144:1149	Gma12p	1144:1149	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	6	59	theme	family	1105:1110	arg1	α1,2-galactosyltransferases					1115:1141	two GT family 34 α1,2-galactosyltransferases	1098:1141	two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p)	1098:1160	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	8	60	theme	α-1,2-linked	1640:1651	arg1	galactose					1653:1661	α-1,2-linked galactose	1640:1661	α-1,2-linked galactose	1640:1661	Furthermore, HPLC and lectin-blot analysis revealed that Otg1p showed α1,3-galactosyltransferase activity under conditions of co-expression with Gmh6p, indicating that α-1,2-linked galactose is required for the galactosylation activity of Otg1p in S. pombe.
33909078	7	61	theme	N-linked	1445:1452	arg1	oligosaccharides					1454:1469	N-linked oligosaccharides	1445:1469	N-linked oligosaccharides	1445:1469	Moreover, 1H-NMR of N-glycans revealed that three GT family 34 α1,2-galactosyltransferases (Gmh1p, Gmh2p and Gmh3p) are required for the galactosylation of N-linked oligosaccharides.
33909078	0	62	theme	Otg1p	99:103	arg1	α1,3-galactosyltransferases					37:63	α1,3-galactosyltransferases	37:63	α1,3-galactosyltransferases	37:63	Substrate specificities of α1,2- and α1,3-galactosyltransferases and characterization of Gmh1p and Otg1p in Schizosaccharomyces pombe.
33909078	0	62	theme	Otg1p	99:103	arg1	α1,2-					27:31	α1,2-	27:31	α1,2-	27:31	Substrate specificities of α1,2- and α1,3-galactosyltransferases and characterization of Gmh1p and Otg1p in Schizosaccharomyces pombe.
33909078	0	62	theme	Otg1p	99:103	arg1	characterization					69:84	characterization	69:84	characterization	69:84	Substrate specificities of α1,2- and α1,3-galactosyltransferases and characterization of Gmh1p and Otg1p in Schizosaccharomyces pombe.
33909078	0	63	from	characterization	69:84	arg1	pombe					128:132	Schizosaccharomyces pombe	108:132	Schizosaccharomyces pombe	108:132	Substrate specificities of α1,2- and α1,3-galactosyltransferases and characterization of Gmh1p and Otg1p in Schizosaccharomyces pombe.
33909078	0	64	from	specificities	10:22	arg1	pombe					128:132	Schizosaccharomyces pombe	108:132	Schizosaccharomyces pombe	108:132	Substrate specificities of α1,2- and α1,3-galactosyltransferases and characterization of Gmh1p and Otg1p in Schizosaccharomyces pombe.
33909078	3	65	contain	has	537:539	arg2	three					639:643	three	639:643	three	639:643	Schizosaccharomyces pombe has 10 galactosyltransferase-related genes: seven belonging to glycosyltransferase (GT) family 34 and three belonging GT family 8.
33909078	3	65	contain	has	537:539	arg1	pombe					531:535	Schizosaccharomyces pombe	511:535	Schizosaccharomyces pombe	511:535	Schizosaccharomyces pombe has 10 galactosyltransferase-related genes: seven belonging to glycosyltransferase (GT) family 34 and three belonging GT family 8.
33909078	3	65	contain	has	537:539	arg2	seven					581:585	seven	581:585	seven	581:585	Schizosaccharomyces pombe has 10 galactosyltransferase-related genes: seven belonging to glycosyltransferase (GT) family 34 and three belonging GT family 8.
33909078	3	65	contain	has	537:539	arg2	genes					574:578	10 galactosyltransferase-related genes	541:578	10 galactosyltransferase-related genes: seven belonging to glycosyltransferase (GT) family 34 and three belonging GT family 8	541:665	Schizosaccharomyces pombe has 10 galactosyltransferase-related genes: seven belonging to glycosyltransferase (GT) family 34 and three belonging GT family 8.
33909078	1	66	dep	yeast	150:154	arg1	pombe					176:180	Schizosaccharomyces pombe	156:180	the fission yeast Schizosaccharomyces pombe	138:180	In the fission yeast Schizosaccharomyces pombe, α1,2- and α1,3-linked D-galactose (Gal) residues are transferred to N- and O-linked oligosaccharides of glycoproteins by galactosyltransferases.
33909078	6	67	theme	GT	1170:1171	arg1	α1,3-galactosyltransferases					1182:1208	two GT family 8 α1,3-galactosyltransferases	1166:1208	two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p)	1166:1226	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	6	67	theme	GT	1170:1171	arg1	Otg3p					1221:1225	Otg3p	1221:1225	Otg3p	1221:1225	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	6	67	theme	GT	1170:1171	arg1	Otg2p					1211:1215	Otg2p	1211:1215	Otg2p	1211:1215	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	0	68	from	α1,3-galactosyltransferases	37:63	arg1	pombe					128:132	Schizosaccharomyces pombe	108:132	Schizosaccharomyces pombe	108:132	Substrate specificities of α1,2- and α1,3-galactosyltransferases and characterization of Gmh1p and Otg1p in Schizosaccharomyces pombe.
33909078	2	69	theme	nonsexual	420:428	arg1	aggregation					430:440	nonsexual aggregation	420:440	nonsexual aggregation	420:440	Although the galactomannans are important for cell-cell communication in S. pombe (e.g., in nonsexual aggregation), the mechanisms underlying galactosylation in cells remain unclear.
33909078	5	70	theme	substrate	838:846	arg1	specificities					848:860	substrate specificities	838:860	substrate specificities	838:860	Here, we have investigated the function and substrate specificities of galactosyltransferases in S pombe by using strains expressing single α-galactosyltransferases in the Δ10GalT background.
33909078	5	71	from	specificities	848:860	arg1	S					891:891	S	891:891	S	891:891	Here, we have investigated the function and substrate specificities of galactosyltransferases in S pombe by using strains expressing single α-galactosyltransferases in the Δ10GalT background.
33909078	3	72	dep	genes	574:578	arg1	three					639:643	three	639:643	three	639:643	Schizosaccharomyces pombe has 10 galactosyltransferase-related genes: seven belonging to glycosyltransferase (GT) family 34 and three belonging GT family 8.
33909078	3	72	dep	genes	574:578	arg1	seven					581:585	seven	581:585	seven	581:585	Schizosaccharomyces pombe has 10 galactosyltransferase-related genes: seven belonging to glycosyltransferase (GT) family 34 and three belonging GT family 8.
33909078	3	72	dep	genes	574:578	arg1	genes					574:578	10 galactosyltransferase-related genes	541:578	10 galactosyltransferase-related genes: seven belonging to glycosyltransferase (GT) family 34 and three belonging GT family 8	541:665	Schizosaccharomyces pombe has 10 galactosyltransferase-related genes: seven belonging to glycosyltransferase (GT) family 34 and three belonging GT family 8.
33909078	7	73	link	N-linked	1445:1452	arg1	oligosaccharides					1454:1469	N-linked oligosaccharides	1445:1469	N-linked oligosaccharides	1445:1469	Moreover, 1H-NMR of N-glycans revealed that three GT family 34 α1,2-galactosyltransferases (Gmh1p, Gmh2p and Gmh3p) are required for the galactosylation of N-linked oligosaccharides.
33909078	1	74	link	α1,3-linked	193:203	arg1	residues					223:230	α1,3-linked D-galactose (Gal) residues	193:230	α1,3-linked D-galactose (Gal) residues	193:230	In the fission yeast Schizosaccharomyces pombe, α1,2- and α1,3-linked D-galactose (Gal) residues are transferred to N- and O-linked oligosaccharides of glycoproteins by galactosyltransferases.
33909078	9	75	contain	have	1793:1796	arg1	galactosyltransferases					1751:1772	eight galactosyltransferases	1745:1772	eight galactosyltransferases	1745:1772	In conclusion, eight galactosyltransferases have been shown to have activity in S. pombe with different substrate specificities.
33909078	9	75	contain	have	1793:1796	arg2	activity					1798:1805	activity	1798:1805	activity in S. pombe with different substrate specificities	1798:1856	In conclusion, eight galactosyltransferases have been shown to have activity in S. pombe with different substrate specificities.
33909078	0	76	theme	α1,2-	27:31	arg1	specificities					10:22	Substrate specificities	0:22	Substrate specificities of α1,2- and α1,3-galactosyltransferases and characterization of Gmh1p and Otg1p in Schizosaccharomyces pombe.	0:133	Substrate specificities of α1,2- and α1,3-galactosyltransferases and characterization of Gmh1p and Otg1p in Schizosaccharomyces pombe.
33909078	8	77	theme	HPLC	1485:1488	arg1	analysis					1506:1513	HPLC and lectin-blot analysis	1485:1513	analysis	1506:1513	Furthermore, HPLC and lectin-blot analysis revealed that Otg1p showed α1,3-galactosyltransferase activity under conditions of co-expression with Gmh6p, indicating that α-1,2-linked galactose is required for the galactosylation activity of Otg1p in S. pombe.
33909078	0	78	theme	α1,3-galactosyltransferases	37:63	arg1	specificities					10:22	Substrate specificities	0:22	Substrate specificities of α1,2- and α1,3-galactosyltransferases and characterization of Gmh1p and Otg1p in Schizosaccharomyces pombe.	0:133	Substrate specificities of α1,2- and α1,3-galactosyltransferases and characterization of Gmh1p and Otg1p in Schizosaccharomyces pombe.
33909078	8	79	theme	lectin-blot	1494:1504	arg1	analysis					1506:1513	HPLC and lectin-blot analysis	1485:1513	analysis	1506:1513	Furthermore, HPLC and lectin-blot analysis revealed that Otg1p showed α1,3-galactosyltransferase activity under conditions of co-expression with Gmh6p, indicating that α-1,2-linked galactose is required for the galactosylation activity of Otg1p in S. pombe.
33909078	6	80	dep	α1,2-galactosyltransferases	1115:1141	arg1	Gmh6p					1155:1159	Gmh6p	1155:1159	Gmh6p	1155:1159	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	6	80	dep	α1,2-galactosyltransferases	1115:1141	arg1	Gma12p					1144:1149	Gma12p	1144:1149	Gma12p	1144:1149	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	6	80	dep	α1,2-galactosyltransferases	1115:1141	arg1	α1,2-galactosyltransferases					1115:1141	two GT family 34 α1,2-galactosyltransferases	1098:1141	two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p)	1098:1160	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	6	81	dep	α1,3-galactosyltransferases	1182:1208	arg1	α1,3-galactosyltransferases					1182:1208	two GT family 8 α1,3-galactosyltransferases	1166:1208	two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p)	1166:1226	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	6	81	dep	α1,3-galactosyltransferases	1182:1208	arg1	Otg3p					1221:1225	Otg3p	1221:1225	Otg3p	1221:1225	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	6	81	dep	α1,3-galactosyltransferases	1182:1208	arg1	Otg2p					1211:1215	Otg2p	1211:1215	Otg2p	1211:1215	High-performance liquid chromatography (HPLC) analysis of pyridylaminated O-linked oligosaccharides showed that two GT family 34 α1,2-galactosyltransferases (Gma12p and Gmh6p) and two GT family 8 α1,3-galactosyltransferases (Otg2p and Otg3p) are involved in galactosylation of O-linked oligosaccharide.
33909078	3	82	theme	galactosyltransferase-related	544:572	arg1	three					639:643	three	639:643	three	639:643	Schizosaccharomyces pombe has 10 galactosyltransferase-related genes: seven belonging to glycosyltransferase (GT) family 34 and three belonging GT family 8.
33909078	3	82	theme	galactosyltransferase-related	544:572	arg1	seven					581:585	seven	581:585	seven	581:585	Schizosaccharomyces pombe has 10 galactosyltransferase-related genes: seven belonging to glycosyltransferase (GT) family 34 and three belonging GT family 8.
33909078	3	82	theme	galactosyltransferase-related	544:572	arg1	genes					574:578	10 galactosyltransferase-related genes	541:578	10 galactosyltransferase-related genes: seven belonging to glycosyltransferase (GT) family 34 and three belonging GT family 8	541:665	Schizosaccharomyces pombe has 10 galactosyltransferase-related genes: seven belonging to glycosyltransferase (GT) family 34 and three belonging GT family 8.
33909078	5	83	dep	S	891:891	arg1	pombe					893:897	S pombe	891:897	S pombe	891:897	Here, we have investigated the function and substrate specificities of galactosyltransferases in S pombe by using strains expressing single α-galactosyltransferases in the Δ10GalT background.
33909078	8	84	link	α-1,2-linked	1640:1651	arg1	galactose					1653:1661	α-1,2-linked galactose	1640:1661	α-1,2-linked galactose	1640:1661	Furthermore, HPLC and lectin-blot analysis revealed that Otg1p showed α1,3-galactosyltransferase activity under conditions of co-expression with Gmh6p, indicating that α-1,2-linked galactose is required for the galactosylation activity of Otg1p in S. pombe.
33909078	7	85	theme	34	1349:1350	arg1	Gmh1p					1381:1385	Gmh1p	1381:1385	Gmh1p	1381:1385	Moreover, 1H-NMR of N-glycans revealed that three GT family 34 α1,2-galactosyltransferases (Gmh1p, Gmh2p and Gmh3p) are required for the galactosylation of N-linked oligosaccharides.
33909078	7	85	theme	34	1349:1350	arg1	α1,2-galactosyltransferases					1352:1378	three GT family 34 α1,2-galactosyltransferases	1333:1378	three GT family 34 α1,2-galactosyltransferases (Gmh1p, Gmh2p and Gmh3p)	1333:1403	Moreover, 1H-NMR of N-glycans revealed that three GT family 34 α1,2-galactosyltransferases (Gmh1p, Gmh2p and Gmh3p) are required for the galactosylation of N-linked oligosaccharides.
33909078	7	85	theme	34	1349:1350	arg1	Gmh3p					1398:1402	Gmh3p	1398:1402	Gmh3p	1398:1402	Moreover, 1H-NMR of N-glycans revealed that three GT family 34 α1,2-galactosyltransferases (Gmh1p, Gmh2p and Gmh3p) are required for the galactosylation of N-linked oligosaccharides.
33909078	7	85	theme	34	1349:1350	arg1	Gmh2p					1388:1392	Gmh2p	1388:1392	Gmh2p	1388:1392	Moreover, 1H-NMR of N-glycans revealed that three GT family 34 α1,2-galactosyltransferases (Gmh1p, Gmh2p and Gmh3p) are required for the galactosylation of N-linked oligosaccharides.
33909078	8	86	theme	α1,3-galactosyltransferase	1542:1567	arg1	activity					1569:1576	α1,3-galactosyltransferase activity	1542:1576	α1,3-galactosyltransferase activity	1542:1576	Furthermore, HPLC and lectin-blot analysis revealed that Otg1p showed α1,3-galactosyltransferase activity under conditions of co-expression with Gmh6p, indicating that α-1,2-linked galactose is required for the galactosylation activity of Otg1p in S. pombe.
32706435	8	0	theme	outer	1418:1422	arg1	surface					1424:1430	the outer surface	1414:1430	the outer surface of the cell using hexose-DolPs as sugar donors	1414:1477	Our results also suggested that the synthesis of the N- and O-linked glycans onto target protein occurs on the outer surface of the cell using hexose-DolPs as sugar donors.
32706435	9	1	theme	defined	1562:1568	arg1	medium					1570:1575	the defined medium	1558:1575	the defined medium mimicking the natural habitat of H. hispanica	1558:1621	Deletion mutant showed that N- and O-glycosylation are required for growth in the defined medium mimicking the natural habitat of H. hispanica.
32706435	0	2	link	lipid-linked	69:80	arg1	intermediates					82:94	the lipid-linked intermediates	65:94	the lipid-linked intermediates	65:94	Agl22 and Agl23 are involved in the synthesis and utilization of the lipid-linked intermediates in the glycosylation pathways of the halophilic archaeaon Haloarcula hispanica.
32706435	3	3	theme	Haloarcula	496:505	arg1	hispanica					507:515	Haloarcula hispanica	496:515	Haloarcula hispanica	496:515	To explore commonalities and variations of these two versions of glycosylation, we used Haloarcula hispanica as a model.
32706435	3	3	theme	Haloarcula	496:505	arg1	model					522:526	a model	520:526	a model	520:526	To explore commonalities and variations of these two versions of glycosylation, we used Haloarcula hispanica as a model.
32706435	4	4	dep	H.	559:560	arg1	hispanica					562:570	H. hispanica	559:570	H. hispanica S-layer glycoproteins	559:592	Our previous work showed that H. hispanica S-layer glycoproteins are modified by an N-linked glucose-α-(1, 2)-[sulfoquinovosamine-β-(1, 6)-]galactose trisaccharide and an O-linked glucose-α-(1, 4)-galactose disaccharide.
32706435	4	5	theme	S-layer	572:578	arg1	glycoproteins					580:592	H. hispanica S-layer glycoproteins	559:592	H. hispanica S-layer glycoproteins	559:592	Our previous work showed that H. hispanica S-layer glycoproteins are modified by an N-linked glucose-α-(1, 2)-[sulfoquinovosamine-β-(1, 6)-]galactose trisaccharide and an O-linked glucose-α-(1, 4)-galactose disaccharide.
32706435	1	6	link	O-linked	253:260	arg1	glycans					262:268	O-linked glycans	253:268	O-linked glycans	253:268	Like both eukaryotes and bacteria, archaea can decorate proteins with N- and O-linked glycans.
32706435	2	7	theme	model	346:350	arg1	organisms					361:369	several model archaeal organisms	338:369	several model archaeal organisms	338:369	Whereas pathways and roles of N-glycosylation have been studied in several model archaeal organisms, little is known of O-glycosylation.
32706435	6	8	theme	phenotype	918:926	arg1	analysis					928:935	phenotype analysis	918:935	phenotype analysis	918:935	As revealed by bioinformatics, gene deletion and phenotype analysis, gene HAH_1571, renamed agl22, encodes a predicted glucosyltransferase that transfers glucose from glucose-DolP onto galactose-DolP to form the glucose-α-(1, 4)-galactose-DolP precursor of the N-glycosylation.
32706435	0	9	theme	intermediates	82:94	arg1	utilization					50:60	utilization	50:60	utilization	50:60	Agl22 and Agl23 are involved in the synthesis and utilization of the lipid-linked intermediates in the glycosylation pathways of the halophilic archaeaon Haloarcula hispanica.
32706435	0	9	theme	intermediates	82:94	arg1	synthesis					36:44	synthesis	36:44	synthesis	36:44	Agl22 and Agl23 are involved in the synthesis and utilization of the lipid-linked intermediates in the glycosylation pathways of the halophilic archaeaon Haloarcula hispanica.
32706435	6	10	theme	-galactose-DolP	1097:1111	arg1	precursor					1113:1121	the glucose-α-(1, 4)-galactose-DolP precursor	1077:1121	precursor	1113:1121	As revealed by bioinformatics, gene deletion and phenotype analysis, gene HAH_1571, renamed agl22, encodes a predicted glucosyltransferase that transfers glucose from glucose-DolP onto galactose-DolP to form the glucose-α-(1, 4)-galactose-DolP precursor of the N-glycosylation.
32706435	1	11	theme	O-linked	253:260	arg1	glycans					262:268	O-linked glycans	253:268	O-linked glycans	253:268	Like both eukaryotes and bacteria, archaea can decorate proteins with N- and O-linked glycans.
32706435	0	12	theme	lipid-linked	69:80	arg1	intermediates					82:94	the lipid-linked intermediates	65:94	the lipid-linked intermediates	65:94	Agl22 and Agl23 are involved in the synthesis and utilization of the lipid-linked intermediates in the glycosylation pathways of the halophilic archaeaon Haloarcula hispanica.
32706435	0	13	from	synthesis	36:44	arg1	pathways					117:124	the glycosylation pathways	99:124	the glycosylation pathways of the halophilic archaeaon Haloarcula hispanica	99:173	Agl22 and Agl23 are involved in the synthesis and utilization of the lipid-linked intermediates in the glycosylation pathways of the halophilic archaeaon Haloarcula hispanica.
32706435	2	14	theme	archaeal	352:359	arg1	organisms					361:369	several model archaeal organisms	338:369	several model archaeal organisms	338:369	Whereas pathways and roles of N-glycosylation have been studied in several model archaeal organisms, little is known of O-glycosylation.
32706435	7	15	theme	putative	1187:1194	arg1	Gene					1147:1150	Gene HAH_2016	1147:1159	Gene HAH_2016	1147:1159	Gene HAH_2016, renamed agl23, encodes a putative flippase-associated protein responsible for flipping of hexose-DolPs across the membrane to face the exterior.
32706435	7	15	theme	putative	1187:1194	arg1	protein					1216:1222	a putative flippase-associated protein	1185:1222	a putative flippase-associated protein responsible for flipping of hexose-DolPs across the membrane to face the exterior	1185:1304	Gene HAH_2016, renamed agl23, encodes a putative flippase-associated protein responsible for flipping of hexose-DolPs across the membrane to face the exterior.
32706435	4	16	mod	modified	598:605	arg1	glycoproteins					580:592	H. hispanica S-layer glycoproteins	559:592	H. hispanica S-layer glycoproteins	559:592	Our previous work showed that H. hispanica S-layer glycoproteins are modified by an N-linked glucose-α-(1, 2)-[sulfoquinovosamine-β-(1, 6)-]galactose trisaccharide and an O-linked glucose-α-(1, 4)-galactose disaccharide.
32706435	4	16	mod	modified	598:605	arg3	trisaccharide					679:691	an N-linked glucose-α-(1, 2)-[sulfoquinovosamine-β-(1, 6)-]galactose trisaccharide	610:691	an N-linked glucose-α-(1, 2)-[sulfoquinovosamine-β-(1, 6)-]galactose trisaccharide	610:691	Our previous work showed that H. hispanica S-layer glycoproteins are modified by an N-linked glucose-α-(1, 2)-[sulfoquinovosamine-β-(1, 6)-]galactose trisaccharide and an O-linked glucose-α-(1, 4)-galactose disaccharide.
32706435	4	16	mod	modified	598:605	arg3	disaccharide					736:747	an O-linked glucose-α-(1, 4)-galactose disaccharide	697:747	disaccharide	736:747	Our previous work showed that H. hispanica S-layer glycoproteins are modified by an N-linked glucose-α-(1, 2)-[sulfoquinovosamine-β-(1, 6)-]galactose trisaccharide and an O-linked glucose-α-(1, 4)-galactose disaccharide.
32706435	7	17	theme	flippase-associated	1196:1214	arg1	Gene					1147:1150	Gene HAH_2016	1147:1159	Gene HAH_2016	1147:1159	Gene HAH_2016, renamed agl23, encodes a putative flippase-associated protein responsible for flipping of hexose-DolPs across the membrane to face the exterior.
32706435	7	17	theme	flippase-associated	1196:1214	arg1	protein					1216:1222	a putative flippase-associated protein	1185:1222	a putative flippase-associated protein responsible for flipping of hexose-DolPs across the membrane to face the exterior	1185:1304	Gene HAH_2016, renamed agl23, encodes a putative flippase-associated protein responsible for flipping of hexose-DolPs across the membrane to face the exterior.
32706435	9	18	theme	natural	1591:1597	arg1	habitat					1599:1605	the natural habitat	1587:1605	the natural habitat of H. hispanica	1587:1621	Deletion mutant showed that N- and O-glycosylation are required for growth in the defined medium mimicking the natural habitat of H. hispanica.
32706435	5	19	contain	contains	792:799	arg2	phosphate					814:822	C60 dolichol phosphate	801:822	C60 dolichol phosphate (DolP)	801:829	Here, we found that H. hispanica membrane contains C60 dolichol phosphate (DolP) as a lipid carrier for glycosylation.
32706435	5	19	contain	contains	792:799	arg1	membrane					783:790	H. hispanica membrane	770:790	H. hispanica membrane	770:790	Here, we found that H. hispanica membrane contains C60 dolichol phosphate (DolP) as a lipid carrier for glycosylation.
32706435	5	19	contain	contains	792:799	arg2	DolP					825:828	DolP	825:828	DolP	825:828	Here, we found that H. hispanica membrane contains C60 dolichol phosphate (DolP) as a lipid carrier for glycosylation.
32706435	5	19	contain	contains	792:799	arg2	carrier					842:848	a lipid carrier	834:848	a lipid carrier for glycosylation	834:866	Here, we found that H. hispanica membrane contains C60 dolichol phosphate (DolP) as a lipid carrier for glycosylation.
32706435	8	20	theme	target	1389:1394	arg1	protein					1396:1402	target protein	1389:1402	target protein	1389:1402	Our results also suggested that the synthesis of the N- and O-linked glycans onto target protein occurs on the outer surface of the cell using hexose-DolPs as sugar donors.
32706435	6	21	theme	predicted	978:986	arg1	gene					938:941	gene HAH_1571	938:950	gene HAH_1571	938:950	As revealed by bioinformatics, gene deletion and phenotype analysis, gene HAH_1571, renamed agl22, encodes a predicted glucosyltransferase that transfers glucose from glucose-DolP onto galactose-DolP to form the glucose-α-(1, 4)-galactose-DolP precursor of the N-glycosylation.
32706435	6	21	theme	predicted	978:986	arg1	glucosyltransferase					988:1006	a predicted glucosyltransferase	976:1006	a predicted glucosyltransferase that transfers glucose from glucose-DolP onto galactose-DolP to form the glucose-α-(1, 4)-galactose-DolP precursor of the N-glycosylation	976:1144	As revealed by bioinformatics, gene deletion and phenotype analysis, gene HAH_1571, renamed agl22, encodes a predicted glucosyltransferase that transfers glucose from glucose-DolP onto galactose-DolP to form the glucose-α-(1, 4)-galactose-DolP precursor of the N-glycosylation.
32706435	9	22	from	growth	1548:1553	arg1	medium					1570:1575	the defined medium	1558:1575	the defined medium mimicking the natural habitat of H. hispanica	1558:1621	Deletion mutant showed that N- and O-glycosylation are required for growth in the defined medium mimicking the natural habitat of H. hispanica.
32706435	9	23	theme	Deletion	1480:1487	arg1	mutant					1489:1494	Deletion mutant	1480:1494	Deletion mutant	1480:1494	Deletion mutant showed that N- and O-glycosylation are required for growth in the defined medium mimicking the natural habitat of H. hispanica.
32706435	8	24	theme	cell	1439:1442	arg1	surface					1424:1430	the outer surface	1414:1430	the outer surface of the cell using hexose-DolPs as sugar donors	1414:1477	Our results also suggested that the synthesis of the N- and O-linked glycans onto target protein occurs on the outer surface of the cell using hexose-DolPs as sugar donors.
32706435	5	25	theme	dolichol	805:812	arg1	carrier					842:848	a lipid carrier	834:848	a lipid carrier for glycosylation	834:866	Here, we found that H. hispanica membrane contains C60 dolichol phosphate (DolP) as a lipid carrier for glycosylation.
32706435	5	25	theme	dolichol	805:812	arg1	DolP					825:828	DolP	825:828	DolP	825:828	Here, we found that H. hispanica membrane contains C60 dolichol phosphate (DolP) as a lipid carrier for glycosylation.
32706435	5	25	theme	dolichol	805:812	arg1	phosphate					814:822	C60 dolichol phosphate	801:822	C60 dolichol phosphate (DolP)	801:829	Here, we found that H. hispanica membrane contains C60 dolichol phosphate (DolP) as a lipid carrier for glycosylation.
32706435	6	26	theme	gene	900:903	arg1	deletion					905:912	gene deletion	900:912	gene deletion	900:912	As revealed by bioinformatics, gene deletion and phenotype analysis, gene HAH_1571, renamed agl22, encodes a predicted glucosyltransferase that transfers glucose from glucose-DolP onto galactose-DolP to form the glucose-α-(1, 4)-galactose-DolP precursor of the N-glycosylation.
32706435	9	27	theme	H.	1610:1611	arg1	hispanica					1613:1621	H. hispanica	1610:1621	H. hispanica	1610:1621	Deletion mutant showed that N- and O-glycosylation are required for growth in the defined medium mimicking the natural habitat of H. hispanica.
32706435	4	28	gly	glycoproteins	580:592	arg1	glycoproteins					580:592	H. hispanica S-layer glycoproteins	559:592	H. hispanica S-layer glycoproteins	559:592	Our previous work showed that H. hispanica S-layer glycoproteins are modified by an N-linked glucose-α-(1, 2)-[sulfoquinovosamine-β-(1, 6)-]galactose trisaccharide and an O-linked glucose-α-(1, 4)-galactose disaccharide.
32706435	2	29	theme	N-glycosylation	301:315	arg1	pathways					279:286	pathways	279:286	pathways	279:286	Whereas pathways and roles of N-glycosylation have been studied in several model archaeal organisms, little is known of O-glycosylation.
32706435	2	29	theme	N-glycosylation	301:315	arg1	roles					292:296	roles	292:296	roles	292:296	Whereas pathways and roles of N-glycosylation have been studied in several model archaeal organisms, little is known of O-glycosylation.
32706435	8	30	link	O-linked	1367:1374	arg1	glycans					1376:1382	O-linked glycans	1367:1382	O-linked glycans	1367:1382	Our results also suggested that the synthesis of the N- and O-linked glycans onto target protein occurs on the outer surface of the cell using hexose-DolPs as sugar donors.
32706435	7	31	dep	face	1288:1291	arg1	exterior					1297:1304	the exterior	1293:1304	face the exterior	1288:1304	Gene HAH_2016, renamed agl23, encodes a putative flippase-associated protein responsible for flipping of hexose-DolPs across the membrane to face the exterior.
32706435	9	32	theme	hispanica	1613:1621	arg1	habitat					1599:1605	the natural habitat	1587:1605	the natural habitat of H. hispanica	1587:1621	Deletion mutant showed that N- and O-glycosylation are required for growth in the defined medium mimicking the natural habitat of H. hispanica.
32706435	4	33	theme	glucose-α-	709:718	arg1	disaccharide					736:747	an O-linked glucose-α-(1, 4)-galactose disaccharide	697:747	disaccharide	736:747	Our previous work showed that H. hispanica S-layer glycoproteins are modified by an N-linked glucose-α-(1, 2)-[sulfoquinovosamine-β-(1, 6)-]galactose trisaccharide and an O-linked glucose-α-(1, 4)-galactose disaccharide.
32706435	3	34	theme	versions	461:468	arg1	variations					437:446	variations	437:446	variations	437:446	To explore commonalities and variations of these two versions of glycosylation, we used Haloarcula hispanica as a model.
32706435	3	34	theme	versions	461:468	arg1	commonalities					419:431	commonalities	419:431	commonalities	419:431	To explore commonalities and variations of these two versions of glycosylation, we used Haloarcula hispanica as a model.
32706435	4	35	dep	galactose	669:677	arg1	-[sulfoquinovosamine-β-					638:660	1, 2)-[sulfoquinovosamine-β-	633:660	-[sulfoquinovosamine-β-	638:660	Our previous work showed that H. hispanica S-layer glycoproteins are modified by an N-linked glucose-α-(1, 2)-[sulfoquinovosamine-β-(1, 6)-]galactose trisaccharide and an O-linked glucose-α-(1, 4)-galactose disaccharide.
32706435	4	36	theme	H.	559:560	arg1	glycoproteins					580:592	H. hispanica S-layer glycoproteins	559:592	H. hispanica S-layer glycoproteins	559:592	Our previous work showed that H. hispanica S-layer glycoproteins are modified by an N-linked glucose-α-(1, 2)-[sulfoquinovosamine-β-(1, 6)-]galactose trisaccharide and an O-linked glucose-α-(1, 4)-galactose disaccharide.
32706435	0	37	theme	glycosylation	103:115	arg1	pathways					117:124	the glycosylation pathways	99:124	the glycosylation pathways of the halophilic archaeaon Haloarcula hispanica	99:173	Agl22 and Agl23 are involved in the synthesis and utilization of the lipid-linked intermediates in the glycosylation pathways of the halophilic archaeaon Haloarcula hispanica.
32706435	4	38	link	N-linked	613:620	arg1	trisaccharide					679:691	an N-linked glucose-α-(1, 2)-[sulfoquinovosamine-β-(1, 6)-]galactose trisaccharide	610:691	an N-linked glucose-α-(1, 2)-[sulfoquinovosamine-β-(1, 6)-]galactose trisaccharide	610:691	Our previous work showed that H. hispanica S-layer glycoproteins are modified by an N-linked glucose-α-(1, 2)-[sulfoquinovosamine-β-(1, 6)-]galactose trisaccharide and an O-linked glucose-α-(1, 4)-galactose disaccharide.
32706435	4	39	theme	O-linked	700:707	arg1	disaccharide					736:747	an O-linked glucose-α-(1, 4)-galactose disaccharide	697:747	disaccharide	736:747	Our previous work showed that H. hispanica S-layer glycoproteins are modified by an N-linked glucose-α-(1, 2)-[sulfoquinovosamine-β-(1, 6)-]galactose trisaccharide and an O-linked glucose-α-(1, 4)-galactose disaccharide.
32706435	5	40	theme	H.	770:771	arg1	membrane					783:790	H. hispanica membrane	770:790	H. hispanica membrane	770:790	Here, we found that H. hispanica membrane contains C60 dolichol phosphate (DolP) as a lipid carrier for glycosylation.
32706435	8	41	dep	N-	1360:1361	arg1	the					1356:1358	the	1356:1358	the	1356:1358	Our results also suggested that the synthesis of the N- and O-linked glycans onto target protein occurs on the outer surface of the cell using hexose-DolPs as sugar donors.
32706435	7	42	theme	responsible	1224:1234	arg1	Gene					1147:1150	Gene HAH_2016	1147:1159	Gene HAH_2016	1147:1159	Gene HAH_2016, renamed agl23, encodes a putative flippase-associated protein responsible for flipping of hexose-DolPs across the membrane to face the exterior.
32706435	7	42	theme	responsible	1224:1234	arg1	protein					1216:1222	a putative flippase-associated protein	1185:1222	a putative flippase-associated protein responsible for flipping of hexose-DolPs across the membrane to face the exterior	1185:1304	Gene HAH_2016, renamed agl23, encodes a putative flippase-associated protein responsible for flipping of hexose-DolPs across the membrane to face the exterior.
32706435	0	43	dep	synthesis	36:44	arg1	the					32:34	the	32:34	the	32:34	Agl22 and Agl23 are involved in the synthesis and utilization of the lipid-linked intermediates in the glycosylation pathways of the halophilic archaeaon Haloarcula hispanica.
32706435	5	44	theme	hispanica	773:781	arg1	membrane					783:790	H. hispanica membrane	770:790	H. hispanica membrane	770:790	Here, we found that H. hispanica membrane contains C60 dolichol phosphate (DolP) as a lipid carrier for glycosylation.
32706435	0	45	from	utilization	50:60	arg1	pathways					117:124	the glycosylation pathways	99:124	the glycosylation pathways of the halophilic archaeaon Haloarcula hispanica	99:173	Agl22 and Agl23 are involved in the synthesis and utilization of the lipid-linked intermediates in the glycosylation pathways of the halophilic archaeaon Haloarcula hispanica.
32706435	0	46	theme	archaeaon	144:152	arg1	hispanica					165:173	the halophilic archaeaon Haloarcula hispanica	129:173	the halophilic archaeaon Haloarcula hispanica	129:173	Agl22 and Agl23 are involved in the synthesis and utilization of the lipid-linked intermediates in the glycosylation pathways of the halophilic archaeaon Haloarcula hispanica.
32706435	8	47	theme	sugar	1466:1470	arg1	hexose-DolPs					1450:1461	hexose-DolPs	1450:1461	hexose-DolPs	1450:1461	Our results also suggested that the synthesis of the N- and O-linked glycans onto target protein occurs on the outer surface of the cell using hexose-DolPs as sugar donors.
32706435	8	47	theme	sugar	1466:1470	arg1	donors					1472:1477	sugar donors	1466:1477	sugar donors	1466:1477	Our results also suggested that the synthesis of the N- and O-linked glycans onto target protein occurs on the outer surface of the cell using hexose-DolPs as sugar donors.
32706435	3	48	used	used	491:494	arg2	we					488:489	we	488:489	we	488:489	To explore commonalities and variations of these two versions of glycosylation, we used Haloarcula hispanica as a model.
32706435	2	49	theme	several	338:344	arg1	organisms					361:369	several model archaeal organisms	338:369	several model archaeal organisms	338:369	Whereas pathways and roles of N-glycosylation have been studied in several model archaeal organisms, little is known of O-glycosylation.
32706435	4	50	theme	previous	533:540	arg1	work					542:545	Our previous work	529:545	Our previous work	529:545	Our previous work showed that H. hispanica S-layer glycoproteins are modified by an N-linked glucose-α-(1, 2)-[sulfoquinovosamine-β-(1, 6)-]galactose trisaccharide and an O-linked glucose-α-(1, 4)-galactose disaccharide.
32706435	0	51	theme	halophilic	133:142	arg1	hispanica					165:173	the halophilic archaeaon Haloarcula hispanica	129:173	the halophilic archaeaon Haloarcula hispanica	129:173	Agl22 and Agl23 are involved in the synthesis and utilization of the lipid-linked intermediates in the glycosylation pathways of the halophilic archaeaon Haloarcula hispanica.
32706435	5	52	theme	lipid	836:840	arg1	carrier					842:848	a lipid carrier	834:848	a lipid carrier for glycosylation	834:866	Here, we found that H. hispanica membrane contains C60 dolichol phosphate (DolP) as a lipid carrier for glycosylation.
32706435	5	52	theme	lipid	836:840	arg1	phosphate					814:822	C60 dolichol phosphate	801:822	C60 dolichol phosphate (DolP)	801:829	Here, we found that H. hispanica membrane contains C60 dolichol phosphate (DolP) as a lipid carrier for glycosylation.
32706435	8	53	theme	O-linked	1367:1374	arg1	glycans					1376:1382	O-linked glycans	1367:1382	O-linked glycans	1367:1382	Our results also suggested that the synthesis of the N- and O-linked glycans onto target protein occurs on the outer surface of the cell using hexose-DolPs as sugar donors.
32706435	0	54	theme	hispanica	165:173	arg1	pathways					117:124	the glycosylation pathways	99:124	the glycosylation pathways of the halophilic archaeaon Haloarcula hispanica	99:173	Agl22 and Agl23 are involved in the synthesis and utilization of the lipid-linked intermediates in the glycosylation pathways of the halophilic archaeaon Haloarcula hispanica.
32706435	6	55	theme	N-glycosylation	1130:1144	arg1	precursor					1113:1121	the glucose-α-(1, 4)-galactose-DolP precursor	1077:1121	precursor	1113:1121	As revealed by bioinformatics, gene deletion and phenotype analysis, gene HAH_1571, renamed agl22, encodes a predicted glucosyltransferase that transfers glucose from glucose-DolP onto galactose-DolP to form the glucose-α-(1, 4)-galactose-DolP precursor of the N-glycosylation.
32706435	4	56	theme	-galactose	725:734	arg1	disaccharide					736:747	an O-linked glucose-α-(1, 4)-galactose disaccharide	697:747	disaccharide	736:747	Our previous work showed that H. hispanica S-layer glycoproteins are modified by an N-linked glucose-α-(1, 2)-[sulfoquinovosamine-β-(1, 6)-]galactose trisaccharide and an O-linked glucose-α-(1, 4)-galactose disaccharide.
32706435	3	57	theme	glycosylation	473:485	arg1	versions					461:468	these two versions	451:468	these two versions of glycosylation	451:485	To explore commonalities and variations of these two versions of glycosylation, we used Haloarcula hispanica as a model.
32706435	6	58	theme	glucose-α-	1081:1090	arg1	precursor					1113:1121	the glucose-α-(1, 4)-galactose-DolP precursor	1077:1121	precursor	1113:1121	As revealed by bioinformatics, gene deletion and phenotype analysis, gene HAH_1571, renamed agl22, encodes a predicted glucosyltransferase that transfers glucose from glucose-DolP onto galactose-DolP to form the glucose-α-(1, 4)-galactose-DolP precursor of the N-glycosylation.
32706435	0	59	theme	Haloarcula	154:163	arg1	hispanica					165:173	the halophilic archaeaon Haloarcula hispanica	129:173	the halophilic archaeaon Haloarcula hispanica	129:173	Agl22 and Agl23 are involved in the synthesis and utilization of the lipid-linked intermediates in the glycosylation pathways of the halophilic archaeaon Haloarcula hispanica.
32706435	8	60	theme	glycans	1376:1382	arg1	synthesis					1343:1351	the synthesis	1339:1351	the synthesis of the N- and O-linked glycans onto target protein	1339:1402	Our results also suggested that the synthesis of the N- and O-linked glycans onto target protein occurs on the outer surface of the cell using hexose-DolPs as sugar donors.
32706435	4	61	dep	-[sulfoquinovosamine-β-	638:660	arg1	6					665:665	6	665:665	6	665:665	Our previous work showed that H. hispanica S-layer glycoproteins are modified by an N-linked glucose-α-(1, 2)-[sulfoquinovosamine-β-(1, 6)-]galactose trisaccharide and an O-linked glucose-α-(1, 4)-galactose disaccharide.
32706435	1	62	with	proteins	232:239	arg1	N-					246:247	N-	246:247	N-	246:247	Like both eukaryotes and bacteria, archaea can decorate proteins with N- and O-linked glycans.
32706435	1	62	with	proteins	232:239	arg1	glycans					262:268	O-linked glycans	253:268	O-linked glycans	253:268	Like both eukaryotes and bacteria, archaea can decorate proteins with N- and O-linked glycans.
32706435	4	63	theme	galactose	669:677	arg1	trisaccharide					679:691	an N-linked glucose-α-(1, 2)-[sulfoquinovosamine-β-(1, 6)-]galactose trisaccharide	610:691	an N-linked glucose-α-(1, 2)-[sulfoquinovosamine-β-(1, 6)-]galactose trisaccharide	610:691	Our previous work showed that H. hispanica S-layer glycoproteins are modified by an N-linked glucose-α-(1, 2)-[sulfoquinovosamine-β-(1, 6)-]galactose trisaccharide and an O-linked glucose-α-(1, 4)-galactose disaccharide.
32706435	7	64	theme	hexose-DolPs	1252:1263	arg1	flipping					1240:1247	flipping	1240:1247	flipping of hexose-DolPs across the membrane to face the exterior	1240:1304	Gene HAH_2016, renamed agl23, encodes a putative flippase-associated protein responsible for flipping of hexose-DolPs across the membrane to face the exterior.
32706435	4	65	theme	N-linked	613:620	arg1	trisaccharide					679:691	an N-linked glucose-α-(1, 2)-[sulfoquinovosamine-β-(1, 6)-]galactose trisaccharide	610:691	an N-linked glucose-α-(1, 2)-[sulfoquinovosamine-β-(1, 6)-]galactose trisaccharide	610:691	Our previous work showed that H. hispanica S-layer glycoproteins are modified by an N-linked glucose-α-(1, 2)-[sulfoquinovosamine-β-(1, 6)-]galactose trisaccharide and an O-linked glucose-α-(1, 4)-galactose disaccharide.
32706435	8	66	theme	N-	1360:1361	arg1	synthesis					1343:1351	the synthesis	1339:1351	the synthesis of the N- and O-linked glycans onto target protein	1339:1402	Our results also suggested that the synthesis of the N- and O-linked glycans onto target protein occurs on the outer surface of the cell using hexose-DolPs as sugar donors.
32706435	6	67	from	glucose-DolP	1036:1047	arg1	glucose					1023:1029	glucose	1023:1029	glucose from glucose-DolP onto galactose-DolP	1023:1067	As revealed by bioinformatics, gene deletion and phenotype analysis, gene HAH_1571, renamed agl22, encodes a predicted glucosyltransferase that transfers glucose from glucose-DolP onto galactose-DolP to form the glucose-α-(1, 4)-galactose-DolP precursor of the N-glycosylation.
32706435	4	68	link	O-linked	700:707	arg1	disaccharide					736:747	an O-linked glucose-α-(1, 4)-galactose disaccharide	697:747	disaccharide	736:747	Our previous work showed that H. hispanica S-layer glycoproteins are modified by an N-linked glucose-α-(1, 2)-[sulfoquinovosamine-β-(1, 6)-]galactose trisaccharide and an O-linked glucose-α-(1, 4)-galactose disaccharide.
32603728	3	0	theme	irradiation	687:697	arg1	~25 kGy					704:710	irradiation dose ~25 kGy	687:710	irradiation dose ~25 kGy	687:710	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	3	0	theme	irradiation	687:697	arg1	conditions					584:593	the optimized conditions	570:593	the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy)	570:711	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	1	1	theme	carbon	297:302	arg1	composite					329:337	a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite	255:337	a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite	255:337	A simple and efficient separation method of carrier-free 47Sc from neutron irradiated titanium target using a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite was established.
32603728	3	2	from	efficiency	481:490	arg1	CS					507:508	CS	507:508	CS	507:508	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	2	3	theme	CS-AA/MWCNTs	377:388	arg1	composite					390:398	the CS-AA/MWCNTs composite	373:398	the CS-AA/MWCNTs composite	373:398	The synthesis of the CS-AA/MWCNTs composite was achieved using gamma radiation-induced template polymerization.
32603728	1	4	from	method	181:186	arg1	target					242:247	neutron irradiated titanium target	214:247	neutron irradiated titanium target	214:247	A simple and efficient separation method of carrier-free 47Sc from neutron irradiated titanium target using a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite was established.
32603728	3	5	theme	maximum	549:555	arg1	%					562:562	a maximum of~84%	547:562	a maximum of~84%	547:562	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	1	6	theme	nanotubes	304:312	arg1	composite					329:337	a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite	255:337	a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite	255:337	A simple and efficient separation method of carrier-free 47Sc from neutron irradiated titanium target using a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite was established.
32603728	0	7	theme	irradiated	119:128	arg1	target					139:144	neutron irradiated titanium target	111:144	neutron irradiated titanium target	111:144	Synthesis of chitosan-acrylic acid/multiwalled carbon nanotubes composite for theranostic 47Sc separation from neutron irradiated titanium target.
32603728	7	8	theme	quality	1233:1239	arg1	tests					1249:1253	The quality control tests	1229:1253	The quality control tests (radionuclidic, radiochemical, and chemical purities) for the eluted 47Sc(III)	1229:1332	The quality control tests (radionuclidic, radiochemical, and chemical purities) for the eluted 47Sc(III) clarified its high purity and validity for cancer theranostics.
32603728	7	8	theme	quality	1233:1239	arg1	purities					1299:1306	radionuclidic, radiochemical, and chemical purities	1256:1306	radionuclidic, radiochemical, and chemical purities	1256:1306	The quality control tests (radionuclidic, radiochemical, and chemical purities) for the eluted 47Sc(III) clarified its high purity and validity for cancer theranostics.
32603728	4	9	theme	new	847:849	arg1	composite					863:871	the new synthesized composite	843:871	the new synthesized composite	843:871	Different analyses (FT-IR, SEM, TGA and DTA) were examined for confirming the structural morphology and mechanical properties of the new synthesized composite.
32603728	7	10	theme	radiochemical	1271:1283	arg1	purities					1299:1306	radionuclidic, radiochemical, and chemical purities	1256:1306	radionuclidic, radiochemical, and chemical purities	1256:1306	The quality control tests (radionuclidic, radiochemical, and chemical purities) for the eluted 47Sc(III) clarified its high purity and validity for cancer theranostics.
32603728	7	10	theme	radiochemical	1271:1283	arg1	tests					1249:1253	The quality control tests	1229:1253	The quality control tests (radionuclidic, radiochemical, and chemical purities) for the eluted 47Sc(III)	1229:1332	The quality control tests (radionuclidic, radiochemical, and chemical purities) for the eluted 47Sc(III) clarified its high purity and validity for cancer theranostics.
32603728	3	11	theme	optimized	574:582	arg1	~25 kGy					704:710	irradiation dose ~25 kGy	687:710	irradiation dose ~25 kGy	687:710	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	3	11	theme	optimized	574:582	arg1	AA					615:616	1.0 wt% AA	607:616	1.0 wt% AA	607:616	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	3	11	theme	optimized	574:582	arg1	 N					646:647	>0.2 wt% N	638:647	>0.2 wt% N	638:647	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	3	11	theme	optimized	574:582	arg1	CS					603:604	30 wt% CS	596:604	30 wt% CS	596:604	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	3	11	theme	optimized	574:582	arg1	f-MWCNTs					628:635	0.15 wt% f-MWCNTs	619:635	0.15 wt% f-MWCNTs	619:635	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	3	11	theme	optimized	574:582	arg1	N'-Methylenebisacrylamide					649:673	N'-Methylenebisacrylamide	649:673	N'-Methylenebisacrylamide (NMBA)	649:680	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	3	11	theme	optimized	574:582	arg1	conditions					584:593	the optimized conditions	570:593	the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy)	570:711	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	0	12	theme	neutron	111:117	arg1	target					139:144	neutron irradiated titanium target	111:144	neutron irradiated titanium target	111:144	Synthesis of chitosan-acrylic acid/multiwalled carbon nanotubes composite for theranostic 47Sc separation from neutron irradiated titanium target.
32603728	6	13	theme	47Sc	1085:1088	arg1	separation					1049:1058	The ionic exchange separation	1030:1058	The ionic exchange separation of no-carrier-added (NCA)47Sc(III) from irradiated TiO2 target on CS-AA/MWCNTs composite packed column	1030:1161	The ionic exchange separation of no-carrier-added (NCA)47Sc(III) from irradiated TiO2 target on CS-AA/MWCNTs composite packed column efficiently eluted 47Sc(III) by 91 ± 0.8% using 1 M HCl solution.
32603728	6	14	theme	TiO2	1111:1114	arg1	target					1116:1121	irradiated TiO2 target	1100:1121	irradiated TiO2 target	1100:1121	The ionic exchange separation of no-carrier-added (NCA)47Sc(III) from irradiated TiO2 target on CS-AA/MWCNTs composite packed column efficiently eluted 47Sc(III) by 91 ± 0.8% using 1 M HCl solution.
32603728	5	15	theme	Sc	951:952	arg1	ions					978:981	Sc(III) rather than Ti(IV) ions	951:981	Sc(III) rather than Ti(IV) ions	951:981	Interestingly, the CS-AA/MWCNTs composite depicted a selective adsorption of Sc(III) rather than Ti(IV) ions at pH 5 with adsorption efficiency of ~93.93%.
32603728	1	16	theme	CS-AA/MWCNTs	315:326	arg1	composite					329:337	a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite	255:337	a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite	255:337	A simple and efficient separation method of carrier-free 47Sc from neutron irradiated titanium target using a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite was established.
32603728	7	17	theme	high	1348:1351	arg1	purity					1353:1358	its high purity	1344:1358	its high purity	1344:1358	The quality control tests (radionuclidic, radiochemical, and chemical purities) for the eluted 47Sc(III) clarified its high purity and validity for cancer theranostics.
32603728	7	18	theme	eluted	1317:1322	arg1	47Sc					1324:1327	the eluted 47Sc	1313:1327	the eluted 47Sc	1313:1327	The quality control tests (radionuclidic, radiochemical, and chemical purities) for the eluted 47Sc(III) clarified its high purity and validity for cancer theranostics.
32603728	5	19	theme	CS-AA/MWCNTs	893:904	arg1	composite					906:914	the CS-AA/MWCNTs composite	889:914	the CS-AA/MWCNTs composite	889:914	Interestingly, the CS-AA/MWCNTs composite depicted a selective adsorption of Sc(III) rather than Ti(IV) ions at pH 5 with adsorption efficiency of ~93.93%.
32603728	1	20	theme	carrier-free	191:202	arg1	47Sc					204:207	carrier-free 47Sc	191:207	carrier-free 47Sc from neutron irradiated titanium target	191:247	A simple and efficient separation method of carrier-free 47Sc from neutron irradiated titanium target using a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite was established.
32603728	0	21	theme	titanium	130:137	arg1	target					139:144	neutron irradiated titanium target	111:144	neutron irradiated titanium target	111:144	Synthesis of chitosan-acrylic acid/multiwalled carbon nanotubes composite for theranostic 47Sc separation from neutron irradiated titanium target.
32603728	3	22	theme	dose	699:702	arg1	~25 kGy					704:710	irradiation dose ~25 kGy	687:710	irradiation dose ~25 kGy	687:710	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	3	22	theme	dose	699:702	arg1	conditions					584:593	the optimized conditions	570:593	the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy)	570:711	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	4	23	theme	structural	792:801	arg1	morphology					803:812	structural morphology	792:812	structural morphology	792:812	Different analyses (FT-IR, SEM, TGA and DTA) were examined for confirming the structural morphology and mechanical properties of the new synthesized composite.
32603728	1	24	theme	47Sc	204:207	arg1	method					181:186	A simple and efficient separation method	147:186	A simple and efficient separation method of carrier-free 47Sc from neutron irradiated titanium target using a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite	147:337	A simple and efficient separation method of carrier-free 47Sc from neutron irradiated titanium target using a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite was established.
32603728	6	25	from	separation	1049:1058	arg1	column					1156:1161	CS-AA/MWCNTs composite packed column	1126:1161	CS-AA/MWCNTs composite packed column	1126:1161	The ionic exchange separation of no-carrier-added (NCA)47Sc(III) from irradiated TiO2 target on CS-AA/MWCNTs composite packed column efficiently eluted 47Sc(III) by 91 ± 0.8% using 1 M HCl solution.
32603728	6	25	from	separation	1049:1058	arg1	target					1116:1121	irradiated TiO2 target	1100:1121	irradiated TiO2 target	1100:1121	The ionic exchange separation of no-carrier-added (NCA)47Sc(III) from irradiated TiO2 target on CS-AA/MWCNTs composite packed column efficiently eluted 47Sc(III) by 91 ± 0.8% using 1 M HCl solution.
32603728	5	26	from	pH 5	986:989	arg1	adsorption					937:946	a selective adsorption	925:946	a selective adsorption of Sc(III) rather than Ti(IV) ions at pH 5	925:989	Interestingly, the CS-AA/MWCNTs composite depicted a selective adsorption of Sc(III) rather than Ti(IV) ions at pH 5 with adsorption efficiency of ~93.93%.
32603728	4	27	theme	mechanical	818:827	arg1	properties					829:838	mechanical properties	818:838	mechanical properties	818:838	Different analyses (FT-IR, SEM, TGA and DTA) were examined for confirming the structural morphology and mechanical properties of the new synthesized composite.
32603728	3	28	theme	%	601:601	arg1	conditions					584:593	the optimized conditions	570:593	the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy)	570:711	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	3	28	theme	%	601:601	arg1	CS					603:604	30 wt% CS	596:604	30 wt% CS	596:604	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	6	29	theme	HCl	1215:1217	arg1	solution					1219:1226	1 M HCl solution	1211:1226	1 M HCl solution	1211:1226	The ionic exchange separation of no-carrier-added (NCA)47Sc(III) from irradiated TiO2 target on CS-AA/MWCNTs composite packed column efficiently eluted 47Sc(III) by 91 ± 0.8% using 1 M HCl solution.
32603728	4	30	dep	analyses	724:731	arg1	SEM					741:743	SEM	741:743	SEM	741:743	Different analyses (FT-IR, SEM, TGA and DTA) were examined for confirming the structural morphology and mechanical properties of the new synthesized composite.
32603728	4	30	dep	analyses	724:731	arg1	FT-IR					734:738	FT-IR	734:738	FT-IR	734:738	Different analyses (FT-IR, SEM, TGA and DTA) were examined for confirming the structural morphology and mechanical properties of the new synthesized composite.
32603728	4	30	dep	analyses	724:731	arg1	analyses					724:731	Different analyses	714:731	Different analyses (FT-IR, SEM, TGA and DTA)	714:757	Different analyses (FT-IR, SEM, TGA and DTA) were examined for confirming the structural morphology and mechanical properties of the new synthesized composite.
32603728	4	30	dep	analyses	724:731	arg1	DTA					754:756	DTA	754:756	DTA	754:756	Different analyses (FT-IR, SEM, TGA and DTA) were examined for confirming the structural morphology and mechanical properties of the new synthesized composite.
32603728	4	30	dep	analyses	724:731	arg1	TGA					746:748	TGA	746:748	TGA	746:748	Different analyses (FT-IR, SEM, TGA and DTA) were examined for confirming the structural morphology and mechanical properties of the new synthesized composite.
32603728	4	31	theme	Different	714:722	arg1	DTA					754:756	DTA	754:756	DTA	754:756	Different analyses (FT-IR, SEM, TGA and DTA) were examined for confirming the structural morphology and mechanical properties of the new synthesized composite.
32603728	4	31	theme	Different	714:722	arg1	SEM					741:743	SEM	741:743	SEM	741:743	Different analyses (FT-IR, SEM, TGA and DTA) were examined for confirming the structural morphology and mechanical properties of the new synthesized composite.
32603728	4	31	theme	Different	714:722	arg1	FT-IR					734:738	FT-IR	734:738	FT-IR	734:738	Different analyses (FT-IR, SEM, TGA and DTA) were examined for confirming the structural morphology and mechanical properties of the new synthesized composite.
32603728	4	31	theme	Different	714:722	arg1	analyses					724:731	Different analyses	714:731	Different analyses (FT-IR, SEM, TGA and DTA)	714:757	Different analyses (FT-IR, SEM, TGA and DTA) were examined for confirming the structural morphology and mechanical properties of the new synthesized composite.
32603728	4	31	theme	Different	714:722	arg1	TGA					746:748	TGA	746:748	TGA	746:748	Different analyses (FT-IR, SEM, TGA and DTA) were examined for confirming the structural morphology and mechanical properties of the new synthesized composite.
32603728	1	32	theme	neutron	214:220	arg1	target					242:247	neutron irradiated titanium target	214:247	neutron irradiated titanium target	214:247	A simple and efficient separation method of carrier-free 47Sc from neutron irradiated titanium target using a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite was established.
32603728	0	33	theme	acid/multiwalled	30:45	arg1	carbon					47:52	chitosan-acrylic acid/multiwalled carbon	13:52	chitosan-acrylic acid/multiwalled carbon	13:52	Synthesis of chitosan-acrylic acid/multiwalled carbon nanotubes composite for theranostic 47Sc separation from neutron irradiated titanium target.
32603728	5	34	theme	%	1027:1027	arg1	efficiency					1007:1016	adsorption efficiency	996:1016	adsorption efficiency of ~93.93%	996:1027	Interestingly, the CS-AA/MWCNTs composite depicted a selective adsorption of Sc(III) rather than Ti(IV) ions at pH 5 with adsorption efficiency of ~93.93%.
32603728	5	35	theme	ions	978:981	arg1	adsorption					937:946	a selective adsorption	925:946	a selective adsorption of Sc(III) rather than Ti(IV) ions at pH 5	925:989	Interestingly, the CS-AA/MWCNTs composite depicted a selective adsorption of Sc(III) rather than Ti(IV) ions at pH 5 with adsorption efficiency of ~93.93%.
32603728	5	36	theme	selective	927:935	arg1	adsorption					937:946	a selective adsorption	925:946	a selective adsorption of Sc(III) rather than Ti(IV) ions at pH 5	925:989	Interestingly, the CS-AA/MWCNTs composite depicted a selective adsorption of Sc(III) rather than Ti(IV) ions at pH 5 with adsorption efficiency of ~93.93%.
32603728	1	37	theme	irradiated	222:231	arg1	target					242:247	neutron irradiated titanium target	214:247	neutron irradiated titanium target	214:247	A simple and efficient separation method of carrier-free 47Sc from neutron irradiated titanium target using a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite was established.
32603728	0	38	theme	chitosan-acrylic	13:28	arg1	carbon					47:52	chitosan-acrylic acid/multiwalled carbon	13:52	chitosan-acrylic acid/multiwalled carbon	13:52	Synthesis of chitosan-acrylic acid/multiwalled carbon nanotubes composite for theranostic 47Sc separation from neutron irradiated titanium target.
32603728	6	39	theme	ionic	1034:1038	arg1	separation					1049:1058	The ionic exchange separation	1030:1058	The ionic exchange separation of no-carrier-added (NCA)47Sc(III) from irradiated TiO2 target on CS-AA/MWCNTs composite packed column	1030:1161	The ionic exchange separation of no-carrier-added (NCA)47Sc(III) from irradiated TiO2 target on CS-AA/MWCNTs composite packed column efficiently eluted 47Sc(III) by 91 ± 0.8% using 1 M HCl solution.
32603728	2	40	theme	radiation-induced	425:441	arg1	polymerization					452:465	gamma radiation-induced template polymerization	419:465	gamma radiation-induced template polymerization	419:465	The synthesis of the CS-AA/MWCNTs composite was achieved using gamma radiation-induced template polymerization.
32603728	3	41	theme	%	645:645	arg1	conditions					584:593	the optimized conditions	570:593	the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy)	570:711	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	3	41	theme	%	645:645	arg1	 N					646:647	>0.2 wt% N	638:647	>0.2 wt% N	638:647	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	3	42	theme	30 wt	596:600	arg1	conditions					584:593	the optimized conditions	570:593	the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy)	570:711	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	3	42	theme	30 wt	596:600	arg1	CS					603:604	30 wt% CS	596:604	30 wt% CS	596:604	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	1	43	theme	titanium	233:240	arg1	target					242:247	neutron irradiated titanium target	214:247	neutron irradiated titanium target	214:247	A simple and efficient separation method of carrier-free 47Sc from neutron irradiated titanium target using a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite was established.
32603728	6	44	theme	no-carrier-added	1063:1078	arg1	47Sc					1085:1088	no-carrier-added (NCA)47Sc(III)	1063:1093	no-carrier-added (NCA)47Sc(III) from irradiated TiO2 target	1063:1121	The ionic exchange separation of no-carrier-added (NCA)47Sc(III) from irradiated TiO2 target on CS-AA/MWCNTs composite packed column efficiently eluted 47Sc(III) by 91 ± 0.8% using 1 M HCl solution.
32603728	6	44	theme	no-carrier-added	1063:1078	arg1	NCA					1081:1083	NCA	1081:1083	NCA	1081:1083	The ionic exchange separation of no-carrier-added (NCA)47Sc(III) from irradiated TiO2 target on CS-AA/MWCNTs composite packed column efficiently eluted 47Sc(III) by 91 ± 0.8% using 1 M HCl solution.
32603728	3	45	theme	%	613:613	arg1	conditions					584:593	the optimized conditions	570:593	the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy)	570:711	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	3	45	theme	%	613:613	arg1	AA					615:616	1.0 wt% AA	607:616	1.0 wt% AA	607:616	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	2	46	theme	template	443:450	arg1	polymerization					452:465	gamma radiation-induced template polymerization	419:465	gamma radiation-induced template polymerization	419:465	The synthesis of the CS-AA/MWCNTs composite was achieved using gamma radiation-induced template polymerization.
32603728	0	47	theme	carbon	47:52	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of chitosan-acrylic acid/multiwalled carbon	0:52	Synthesis of chitosan-acrylic acid/multiwalled carbon nanotubes composite for theranostic 47Sc separation from neutron irradiated titanium target.
32603728	3	48	theme	GE	493:494	arg1	efficiency					481:490	The grafting efficiency	468:490	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs	468:537	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	3	48	theme	GE	493:494	arg1	%					495:495	GE%	493:495	GE%	493:495	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	4	49	dep	morphology	803:812	arg1	the					788:790	the	788:790	the	788:790	Different analyses (FT-IR, SEM, TGA and DTA) were examined for confirming the structural morphology and mechanical properties of the new synthesized composite.
32603728	1	50	from	target	242:247	arg1	method					181:186	A simple and efficient separation method	147:186	A simple and efficient separation method of carrier-free 47Sc from neutron irradiated titanium target using a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite	147:337	A simple and efficient separation method of carrier-free 47Sc from neutron irradiated titanium target using a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite was established.
32603728	1	50	from	target	242:247	arg1	47Sc					204:207	carrier-free 47Sc	191:207	carrier-free 47Sc from neutron irradiated titanium target	191:247	A simple and efficient separation method of carrier-free 47Sc from neutron irradiated titanium target using a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite was established.
32603728	3	51	theme	grafting	472:479	arg1	efficiency					481:490	The grafting efficiency	468:490	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs	468:537	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	3	51	theme	grafting	472:479	arg1	%					495:495	GE%	493:495	GE%	493:495	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	4	52	theme	composite	863:871	arg1	morphology					803:812	structural morphology	792:812	structural morphology	792:812	Different analyses (FT-IR, SEM, TGA and DTA) were examined for confirming the structural morphology and mechanical properties of the new synthesized composite.
32603728	4	52	theme	composite	863:871	arg1	properties					829:838	mechanical properties	818:838	mechanical properties	818:838	Different analyses (FT-IR, SEM, TGA and DTA) were examined for confirming the structural morphology and mechanical properties of the new synthesized composite.
32603728	3	53	theme	>0.2 wt	638:644	arg1	conditions					584:593	the optimized conditions	570:593	the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy)	570:711	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	3	53	theme	>0.2 wt	638:644	arg1	 N					646:647	>0.2 wt% N	638:647	>0.2 wt% N	638:647	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	6	54	theme	packed	1149:1154	arg1	column					1156:1161	CS-AA/MWCNTs composite packed column	1126:1161	CS-AA/MWCNTs composite packed column	1126:1161	The ionic exchange separation of no-carrier-added (NCA)47Sc(III) from irradiated TiO2 target on CS-AA/MWCNTs composite packed column efficiently eluted 47Sc(III) by 91 ± 0.8% using 1 M HCl solution.
32603728	6	55	theme	exchange	1040:1047	arg1	separation					1049:1058	The ionic exchange separation	1030:1058	The ionic exchange separation of no-carrier-added (NCA)47Sc(III) from irradiated TiO2 target on CS-AA/MWCNTs composite packed column	1030:1161	The ionic exchange separation of no-carrier-added (NCA)47Sc(III) from irradiated TiO2 target on CS-AA/MWCNTs composite packed column efficiently eluted 47Sc(III) by 91 ± 0.8% using 1 M HCl solution.
32603728	3	56	theme	f-MWCNTs	530:537	arg1	surface					519:525	the surface	515:525	the surface of f-MWCNTs	515:537	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	3	57	dep	conditions	584:593	arg1	~25 kGy					704:710	irradiation dose ~25 kGy	687:710	irradiation dose ~25 kGy	687:710	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	3	57	dep	conditions	584:593	arg1	AA					615:616	1.0 wt% AA	607:616	1.0 wt% AA	607:616	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	3	57	dep	conditions	584:593	arg1	 N					646:647	>0.2 wt% N	638:647	>0.2 wt% N	638:647	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	3	57	dep	conditions	584:593	arg1	CS					603:604	30 wt% CS	596:604	30 wt% CS	596:604	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	3	57	dep	conditions	584:593	arg1	f-MWCNTs					628:635	0.15 wt% f-MWCNTs	619:635	0.15 wt% f-MWCNTs	619:635	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	3	57	dep	conditions	584:593	arg1	N'-Methylenebisacrylamide					649:673	N'-Methylenebisacrylamide	649:673	N'-Methylenebisacrylamide (NMBA)	649:680	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	3	57	dep	conditions	584:593	arg1	conditions					584:593	the optimized conditions	570:593	the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy)	570:711	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	3	57	dep	conditions	584:593	arg1	NMBA					676:679	NMBA	676:679	NMBA	676:679	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	4	58	theme	synthesized	851:861	arg1	composite					863:871	the new synthesized composite	843:871	the new synthesized composite	843:871	Different analyses (FT-IR, SEM, TGA and DTA) were examined for confirming the structural morphology and mechanical properties of the new synthesized composite.
32603728	0	59	from	target	139:144	arg1	separation					95:104	theranostic 47Sc separation	78:104	theranostic 47Sc separation from neutron irradiated titanium target	78:144	Synthesis of chitosan-acrylic acid/multiwalled carbon nanotubes composite for theranostic 47Sc separation from neutron irradiated titanium target.
32603728	3	60	theme	1.0 wt	607:612	arg1	conditions					584:593	the optimized conditions	570:593	the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy)	570:711	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	3	60	theme	1.0 wt	607:612	arg1	AA					615:616	1.0 wt% AA	607:616	1.0 wt% AA	607:616	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	7	61	theme	control	1241:1247	arg1	tests					1249:1253	The quality control tests	1229:1253	The quality control tests (radionuclidic, radiochemical, and chemical purities) for the eluted 47Sc(III)	1229:1332	The quality control tests (radionuclidic, radiochemical, and chemical purities) for the eluted 47Sc(III) clarified its high purity and validity for cancer theranostics.
32603728	7	61	theme	control	1241:1247	arg1	purities					1299:1306	radionuclidic, radiochemical, and chemical purities	1256:1306	radionuclidic, radiochemical, and chemical purities	1256:1306	The quality control tests (radionuclidic, radiochemical, and chemical purities) for the eluted 47Sc(III) clarified its high purity and validity for cancer theranostics.
32603728	2	62	theme	composite	390:398	arg1	synthesis					360:368	The synthesis	356:368	The synthesis of the CS-AA/MWCNTs composite	356:398	The synthesis of the CS-AA/MWCNTs composite was achieved using gamma radiation-induced template polymerization.
32603728	6	63	theme	composite	1139:1147	arg1	column					1156:1161	CS-AA/MWCNTs composite packed column	1126:1161	CS-AA/MWCNTs composite packed column	1126:1161	The ionic exchange separation of no-carrier-added (NCA)47Sc(III) from irradiated TiO2 target on CS-AA/MWCNTs composite packed column efficiently eluted 47Sc(III) by 91 ± 0.8% using 1 M HCl solution.
32603728	5	64	theme	Ti	971:972	arg1	ions					978:981	Sc(III) rather than Ti(IV) ions	951:981	Sc(III) rather than Ti(IV) ions	951:981	Interestingly, the CS-AA/MWCNTs composite depicted a selective adsorption of Sc(III) rather than Ti(IV) ions at pH 5 with adsorption efficiency of ~93.93%.
32603728	6	65	theme	1 M	1211:1213	arg1	solution					1219:1226	1 M HCl solution	1211:1226	1 M HCl solution	1211:1226	The ionic exchange separation of no-carrier-added (NCA)47Sc(III) from irradiated TiO2 target on CS-AA/MWCNTs composite packed column efficiently eluted 47Sc(III) by 91 ± 0.8% using 1 M HCl solution.
32603728	6	66	theme	CS-AA/MWCNTs	1126:1137	arg1	column					1156:1161	CS-AA/MWCNTs composite packed column	1126:1161	CS-AA/MWCNTs composite packed column	1126:1161	The ionic exchange separation of no-carrier-added (NCA)47Sc(III) from irradiated TiO2 target on CS-AA/MWCNTs composite packed column efficiently eluted 47Sc(III) by 91 ± 0.8% using 1 M HCl solution.
32603728	3	67	theme	%	626:626	arg1	conditions					584:593	the optimized conditions	570:593	the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy)	570:711	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	3	67	theme	%	626:626	arg1	f-MWCNTs					628:635	0.15 wt% f-MWCNTs	619:635	0.15 wt% f-MWCNTs	619:635	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	7	68	theme	chemical	1290:1297	arg1	purities					1299:1306	radionuclidic, radiochemical, and chemical purities	1256:1306	radionuclidic, radiochemical, and chemical purities	1256:1306	The quality control tests (radionuclidic, radiochemical, and chemical purities) for the eluted 47Sc(III) clarified its high purity and validity for cancer theranostics.
32603728	7	68	theme	chemical	1290:1297	arg1	tests					1249:1253	The quality control tests	1229:1253	The quality control tests (radionuclidic, radiochemical, and chemical purities) for the eluted 47Sc(III)	1229:1332	The quality control tests (radionuclidic, radiochemical, and chemical purities) for the eluted 47Sc(III) clarified its high purity and validity for cancer theranostics.
32603728	1	69	theme	simple	149:154	arg1	method					181:186	A simple and efficient separation method	147:186	A simple and efficient separation method of carrier-free 47Sc from neutron irradiated titanium target using a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite	147:337	A simple and efficient separation method of carrier-free 47Sc from neutron irradiated titanium target using a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite was established.
32603728	7	70	theme	cancer	1377:1382	arg1	theranostics					1384:1395	cancer theranostics	1377:1395	cancer theranostics	1377:1395	The quality control tests (radionuclidic, radiochemical, and chemical purities) for the eluted 47Sc(III) clarified its high purity and validity for cancer theranostics.
32603728	6	71	theme	irradiated	1100:1109	arg1	target					1116:1121	irradiated TiO2 target	1100:1121	irradiated TiO2 target	1100:1121	The ionic exchange separation of no-carrier-added (NCA)47Sc(III) from irradiated TiO2 target on CS-AA/MWCNTs composite packed column efficiently eluted 47Sc(III) by 91 ± 0.8% using 1 M HCl solution.
32603728	2	72	theme	gamma	419:423	arg1	polymerization					452:465	gamma radiation-induced template polymerization	419:465	gamma radiation-induced template polymerization	419:465	The synthesis of the CS-AA/MWCNTs composite was achieved using gamma radiation-induced template polymerization.
32603728	1	73	theme	novel	257:261	arg1	composite					329:337	a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite	255:337	a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite	255:337	A simple and efficient separation method of carrier-free 47Sc from neutron irradiated titanium target using a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite was established.
32603728	0	74	theme	47Sc	90:93	arg1	separation					95:104	theranostic 47Sc separation	78:104	theranostic 47Sc separation from neutron irradiated titanium target	78:144	Synthesis of chitosan-acrylic acid/multiwalled carbon nanotubes composite for theranostic 47Sc separation from neutron irradiated titanium target.
32603728	5	75	theme	adsorption	996:1005	arg1	efficiency					1007:1016	adsorption efficiency	996:1016	adsorption efficiency of ~93.93%	996:1027	Interestingly, the CS-AA/MWCNTs composite depicted a selective adsorption of Sc(III) rather than Ti(IV) ions at pH 5 with adsorption efficiency of ~93.93%.
32603728	1	76	theme	efficient	160:168	arg1	method					181:186	A simple and efficient separation method	147:186	A simple and efficient separation method of carrier-free 47Sc from neutron irradiated titanium target using a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite	147:337	A simple and efficient separation method of carrier-free 47Sc from neutron irradiated titanium target using a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite was established.
32603728	3	77	theme	AA	501:502	arg1	efficiency					481:490	The grafting efficiency	468:490	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs	468:537	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	3	77	theme	AA	501:502	arg1	%					495:495	GE%	493:495	GE%	493:495	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	1	78	theme	chitosan-acrylic	263:278	arg1	composite					329:337	a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite	255:337	a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite	255:337	A simple and efficient separation method of carrier-free 47Sc from neutron irradiated titanium target using a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite was established.
32603728	0	79	theme	theranostic	78:88	arg1	separation					95:104	theranostic 47Sc separation	78:104	theranostic 47Sc separation from neutron irradiated titanium target	78:144	Synthesis of chitosan-acrylic acid/multiwalled carbon nanotubes composite for theranostic 47Sc separation from neutron irradiated titanium target.
32603728	7	80	theme	radionuclidic	1256:1268	arg1	purities					1299:1306	radionuclidic, radiochemical, and chemical purities	1256:1306	radionuclidic, radiochemical, and chemical purities	1256:1306	The quality control tests (radionuclidic, radiochemical, and chemical purities) for the eluted 47Sc(III) clarified its high purity and validity for cancer theranostics.
32603728	7	80	theme	radionuclidic	1256:1268	arg1	tests					1249:1253	The quality control tests	1229:1253	The quality control tests (radionuclidic, radiochemical, and chemical purities) for the eluted 47Sc(III)	1229:1332	The quality control tests (radionuclidic, radiochemical, and chemical purities) for the eluted 47Sc(III) clarified its high purity and validity for cancer theranostics.
32603728	3	81	theme	0.15 wt	619:625	arg1	conditions					584:593	the optimized conditions	570:593	the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy)	570:711	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	3	81	theme	0.15 wt	619:625	arg1	f-MWCNTs					628:635	0.15 wt% f-MWCNTs	619:635	0.15 wt% f-MWCNTs	619:635	The grafting efficiency (GE%) of AA on CS onto the surface of f-MWCNTs reached a maximum of~84% under the optimized conditions (30 wt% CS, 1.0 wt% AA, 0.15 wt% f-MWCNTs, >0.2 wt% N,N'-Methylenebisacrylamide (NMBA), and irradiation dose ~25 kGy).
32603728	1	82	theme	separation	170:179	arg1	method					181:186	A simple and efficient separation method	147:186	A simple and efficient separation method of carrier-free 47Sc from neutron irradiated titanium target using a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite	147:337	A simple and efficient separation method of carrier-free 47Sc from neutron irradiated titanium target using a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite was established.
32603728	6	83	from	target	1116:1121	arg1	47Sc					1085:1088	no-carrier-added (NCA)47Sc(III)	1063:1093	no-carrier-added (NCA)47Sc(III) from irradiated TiO2 target	1063:1121	The ionic exchange separation of no-carrier-added (NCA)47Sc(III) from irradiated TiO2 target on CS-AA/MWCNTs composite packed column efficiently eluted 47Sc(III) by 91 ± 0.8% using 1 M HCl solution.
32603728	6	83	from	target	1116:1121	arg1	NCA					1081:1083	NCA	1081:1083	NCA	1081:1083	The ionic exchange separation of no-carrier-added (NCA)47Sc(III) from irradiated TiO2 target on CS-AA/MWCNTs composite packed column efficiently eluted 47Sc(III) by 91 ± 0.8% using 1 M HCl solution.
32603728	6	83	from	target	1116:1121	arg1	separation					1049:1058	The ionic exchange separation	1030:1058	The ionic exchange separation of no-carrier-added (NCA)47Sc(III) from irradiated TiO2 target on CS-AA/MWCNTs composite packed column	1030:1161	The ionic exchange separation of no-carrier-added (NCA)47Sc(III) from irradiated TiO2 target on CS-AA/MWCNTs composite packed column efficiently eluted 47Sc(III) by 91 ± 0.8% using 1 M HCl solution.
32603728	1	84	theme	acid/multiwalled	280:295	arg1	composite					329:337	a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite	255:337	a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite	255:337	A simple and efficient separation method of carrier-free 47Sc from neutron irradiated titanium target using a novel chitosan-acrylic acid/multiwalled carbon nanotubes (CS-AA/MWCNTs) composite was established.
33278440	0	0	theme	mucosa	99:104	arg1	cells					111:115	encapsulated human olfactory mucosa stem cells	70:115	the encapsulated human olfactory mucosa stem cells bioactivity for potential nerve regeneration application	66:172	Alginate-magnetic short nanofibers 3D composite hydrogel enhances the encapsulated human olfactory mucosa stem cells bioactivity for potential nerve regeneration application.
33278440	10	1	theme	OE-MSCs	1539:1545	arg1	differentiation					1520:1534	neural-like differentiation	1508:1534	neural-like differentiation of OE-MSCs rather than the sample without SPIONs	1508:1583	The presence of SPIONs in the short nanofibers can accelerate neural-like differentiation of OE-MSCs rather than the sample without SPIONs.
33278440	5	2	from	hydrogels	956:964	arg1	embedded					935:942	embedded	935:942	embedded	935:942	Wet-electrospun gelatin and superparamagnetic iron oxide nanoparticles (SPIONs) nanocomposite nanofibers were chopped using sonication under optimized conditions and subsequently embedded in alginate hydrogels.
33278440	6	3	theme	storage	971:977	arg1	modulus					979:985	The storage modulus	967:985	The storage modulus of hydrogel without M.SNFs as well as with 1 and 5 mg/mL of M.SNFs	967:1052	The storage modulus of hydrogel without M.SNFs as well as with 1 and 5 mg/mL of M.SNFs were in the range of nerve tissue.
33278440	6	4	theme	hydrogel	990:997	arg1	modulus					979:985	The storage modulus	967:985	The storage modulus of hydrogel without M.SNFs as well as with 1 and 5 mg/mL of M.SNFs	967:1052	The storage modulus of hydrogel without M.SNFs as well as with 1 and 5 mg/mL of M.SNFs were in the range of nerve tissue.
33278440	0	5	theme	olfactory	89:97	arg1	cells					111:115	encapsulated human olfactory mucosa stem cells	70:115	the encapsulated human olfactory mucosa stem cells bioactivity for potential nerve regeneration application	66:172	Alginate-magnetic short nanofibers 3D composite hydrogel enhances the encapsulated human olfactory mucosa stem cells bioactivity for potential nerve regeneration application.
33278440	4	6	theme	alginate	603:610	arg1	hydrogels					612:620	alginate hydrogels	603:620	alginate hydrogels incorporating different magnetic short nanofibers (M.SNFs) content for olfactory ecto-mesenchymal stem cells (OE-MSCs) encapsulation	603:753	In this paper, we fabricated alginate hydrogels incorporating different magnetic short nanofibers (M.SNFs) content for olfactory ecto-mesenchymal stem cells (OE-MSCs) encapsulation.
33278440	0	7	theme	cells	111:115	arg1	bioactivity					117:127	the encapsulated human olfactory mucosa stem cells bioactivity	66:127	the encapsulated human olfactory mucosa stem cells bioactivity for potential nerve regeneration application	66:172	Alginate-magnetic short nanofibers 3D composite hydrogel enhances the encapsulated human olfactory mucosa stem cells bioactivity for potential nerve regeneration application.
33278440	9	8	theme	proliferation	1364:1376	arg1	rate					1378:1381	the proliferation rate	1360:1381	the proliferation rate	1360:1381	Moreover, the proliferation rate was enhanced in M.SNF/hydrogels compared to alginate hydrogel.
33278440	7	9	used	used	1126:1129	arg2	new					1136:1138	new	1136:1138	new	1136:1138	For cell encapsulation, OE-MSCs were used as a new hope for neuronal regeneration due to their neural crest origin.
33278440	7	9	used	used	1126:1129	arg2	OE-MSCs					1113:1119	OE-MSCs	1113:1119	OE-MSCs	1113:1119	For cell encapsulation, OE-MSCs were used as a new hope for neuronal regeneration due to their neural crest origin.
33278440	4	10	theme	ecto-mesenchymal	703:718	arg1	OE-MSCs					732:738	OE-MSCs	732:738	OE-MSCs	732:738	In this paper, we fabricated alginate hydrogels incorporating different magnetic short nanofibers (M.SNFs) content for olfactory ecto-mesenchymal stem cells (OE-MSCs) encapsulation.
33278440	4	10	theme	ecto-mesenchymal	703:718	arg1	cells					725:729	olfactory ecto-mesenchymal stem cells	693:729	olfactory ecto-mesenchymal stem cells (OE-MSCs) encapsulation	693:753	In this paper, we fabricated alginate hydrogels incorporating different magnetic short nanofibers (M.SNFs) content for olfactory ecto-mesenchymal stem cells (OE-MSCs) encapsulation.
33278440	1	11	theme	interest	274:281	arg1	design					179:184	The design	175:184	The design of 3D hydrogel constructs to elicit highly controlled cell response	175:252	The design of 3D hydrogel constructs to elicit highly controlled cell response is a major field of interest in developing tissue engineering.
33278440	1	11	theme	interest	274:281	arg1	field					265:269	a major field	257:269	a major field of interest in developing tissue engineering	257:314	The design of 3D hydrogel constructs to elicit highly controlled cell response is a major field of interest in developing tissue engineering.
33278440	0	12	theme	stem	106:109	arg1	cells					111:115	encapsulated human olfactory mucosa stem cells	70:115	the encapsulated human olfactory mucosa stem cells bioactivity for potential nerve regeneration application	66:172	Alginate-magnetic short nanofibers 3D composite hydrogel enhances the encapsulated human olfactory mucosa stem cells bioactivity for potential nerve regeneration application.
33278440	5	13	theme	Wet-electrospun	756:770	arg1	gelatin					772:778	Wet-electrospun gelatin	756:778	Wet-electrospun gelatin	756:778	Wet-electrospun gelatin and superparamagnetic iron oxide nanoparticles (SPIONs) nanocomposite nanofibers were chopped using sonication under optimized conditions and subsequently embedded in alginate hydrogels.
33278440	10	14	theme	neural-like	1508:1518	arg1	differentiation					1520:1534	neural-like differentiation	1508:1534	neural-like differentiation of OE-MSCs rather than the sample without SPIONs	1508:1583	The presence of SPIONs in the short nanofibers can accelerate neural-like differentiation of OE-MSCs rather than the sample without SPIONs.
33278440	10	15	theme	SPIONs	1462:1467	arg1	presence					1450:1457	The presence	1446:1457	The presence of SPIONs in the short nanofibers	1446:1491	The presence of SPIONs in the short nanofibers can accelerate neural-like differentiation of OE-MSCs rather than the sample without SPIONs.
33278440	5	16	theme	alginate	947:954	arg1	hydrogels					956:964	alginate hydrogels	947:964	alginate hydrogels	947:964	Wet-electrospun gelatin and superparamagnetic iron oxide nanoparticles (SPIONs) nanocomposite nanofibers were chopped using sonication under optimized conditions and subsequently embedded in alginate hydrogels.
33278440	5	17	theme	oxide	807:811	arg1	SPIONs					828:833	SPIONs	828:833	SPIONs	828:833	Wet-electrospun gelatin and superparamagnetic iron oxide nanoparticles (SPIONs) nanocomposite nanofibers were chopped using sonication under optimized conditions and subsequently embedded in alginate hydrogels.
33278440	5	17	theme	oxide	807:811	arg1	nanoparticles					813:825	superparamagnetic iron oxide nanoparticles	784:825	superparamagnetic iron oxide nanoparticles (SPIONs)	784:834	Wet-electrospun gelatin and superparamagnetic iron oxide nanoparticles (SPIONs) nanocomposite nanofibers were chopped using sonication under optimized conditions and subsequently embedded in alginate hydrogels.
33278440	3	18	theme	cell	512:515	arg1	environment					526:536	a cell friendly environment	510:536	a cell friendly environment	510:536	Combining short nanofibers within a hydrogel serves as a promising method to develop a cell friendly environment mimicking the extracellular matrix.
33278440	8	19	theme	LIVE/DEAD	1228:1236	arg1	staining					1238:1245	LIVE/DEAD staining	1228:1245	LIVE/DEAD staining	1228:1245	Resazurin analyses and LIVE/DEAD staining confirmed that the composite hydrogels containing M.SNFs can preserve the cell viability after 7 days.
33278440	2	20	theme	relatively	403:412	arg1	inertness					414:422	its relatively inertness	399:422	its relatively inertness	399:422	The bioactivity of encapsulated cells inside pure alginate hydrogel is limited by its relatively inertness.
33278440	1	21	theme	3D	189:190	arg1	constructs					201:210	3D hydrogel constructs	189:210	3D hydrogel constructs	189:210	The design of 3D hydrogel constructs to elicit highly controlled cell response is a major field of interest in developing tissue engineering.
33278440	7	22	theme	crest	1191:1195	arg1	origin					1197:1202	their neural crest origin	1178:1202	their neural crest origin	1178:1202	For cell encapsulation, OE-MSCs were used as a new hope for neuronal regeneration due to their neural crest origin.
33278440	7	23	theme	neuronal	1149:1156	arg1	regeneration					1158:1169	neuronal regeneration	1149:1169	neuronal regeneration due to their neural crest origin	1149:1202	For cell encapsulation, OE-MSCs were used as a new hope for neuronal regeneration due to their neural crest origin.
33278440	1	24	theme	hydrogel	192:199	arg1	constructs					201:210	3D hydrogel constructs	189:210	3D hydrogel constructs	189:210	The design of 3D hydrogel constructs to elicit highly controlled cell response is a major field of interest in developing tissue engineering.
33278440	0	25	theme	short	18:22	arg1	hydrogel					48:55	Alginate-magnetic short nanofibers 3D composite hydrogel	0:55	Alginate-magnetic short nanofibers 3D composite hydrogel	0:55	Alginate-magnetic short nanofibers 3D composite hydrogel enhances the encapsulated human olfactory mucosa stem cells bioactivity for potential nerve regeneration application.
33278440	5	26	theme	nanocomposite	836:848	arg1	nanofibers					850:859	nanocomposite nanofibers	836:859	nanocomposite nanofibers	836:859	Wet-electrospun gelatin and superparamagnetic iron oxide nanoparticles (SPIONs) nanocomposite nanofibers were chopped using sonication under optimized conditions and subsequently embedded in alginate hydrogels.
33278440	5	27	dep	gelatin	772:778	arg1	nanofibers					850:859	nanocomposite nanofibers	836:859	nanocomposite nanofibers	836:859	Wet-electrospun gelatin and superparamagnetic iron oxide nanoparticles (SPIONs) nanocomposite nanofibers were chopped using sonication under optimized conditions and subsequently embedded in alginate hydrogels.
33278440	2	28	theme	encapsulated	336:347	arg1	cells					349:353	encapsulated cells	336:353	encapsulated cells inside pure alginate hydrogel	336:383	The bioactivity of encapsulated cells inside pure alginate hydrogel is limited by its relatively inertness.
33278440	1	29	theme	constructs	201:210	arg1	design					179:184	The design	175:184	The design of 3D hydrogel constructs to elicit highly controlled cell response	175:252	The design of 3D hydrogel constructs to elicit highly controlled cell response is a major field of interest in developing tissue engineering.
33278440	1	29	theme	constructs	201:210	arg1	field					265:269	a major field	257:269	a major field of interest in developing tissue engineering	257:314	The design of 3D hydrogel constructs to elicit highly controlled cell response is a major field of interest in developing tissue engineering.
33278440	0	30	theme	Alginate-magnetic	0:16	arg1	hydrogel					48:55	Alginate-magnetic short nanofibers 3D composite hydrogel	0:55	Alginate-magnetic short nanofibers 3D composite hydrogel	0:55	Alginate-magnetic short nanofibers 3D composite hydrogel enhances the encapsulated human olfactory mucosa stem cells bioactivity for potential nerve regeneration application.
33278440	10	31	theme	sample	1563:1568	arg1	differentiation					1520:1534	neural-like differentiation	1508:1534	neural-like differentiation of OE-MSCs rather than the sample without SPIONs	1508:1583	The presence of SPIONs in the short nanofibers can accelerate neural-like differentiation of OE-MSCs rather than the sample without SPIONs.
33278440	5	32	theme	superparamagnetic	784:800	arg1	SPIONs					828:833	SPIONs	828:833	SPIONs	828:833	Wet-electrospun gelatin and superparamagnetic iron oxide nanoparticles (SPIONs) nanocomposite nanofibers were chopped using sonication under optimized conditions and subsequently embedded in alginate hydrogels.
33278440	5	32	theme	superparamagnetic	784:800	arg1	nanoparticles					813:825	superparamagnetic iron oxide nanoparticles	784:825	superparamagnetic iron oxide nanoparticles (SPIONs)	784:834	Wet-electrospun gelatin and superparamagnetic iron oxide nanoparticles (SPIONs) nanocomposite nanofibers were chopped using sonication under optimized conditions and subsequently embedded in alginate hydrogels.
33278440	4	33	theme	stem	720:723	arg1	OE-MSCs					732:738	OE-MSCs	732:738	OE-MSCs	732:738	In this paper, we fabricated alginate hydrogels incorporating different magnetic short nanofibers (M.SNFs) content for olfactory ecto-mesenchymal stem cells (OE-MSCs) encapsulation.
33278440	4	33	theme	stem	720:723	arg1	cells					725:729	olfactory ecto-mesenchymal stem cells	693:729	olfactory ecto-mesenchymal stem cells (OE-MSCs) encapsulation	693:753	In this paper, we fabricated alginate hydrogels incorporating different magnetic short nanofibers (M.SNFs) content for olfactory ecto-mesenchymal stem cells (OE-MSCs) encapsulation.
33278440	0	34	theme	3D	35:36	arg1	hydrogel					48:55	Alginate-magnetic short nanofibers 3D composite hydrogel	0:55	Alginate-magnetic short nanofibers 3D composite hydrogel	0:55	Alginate-magnetic short nanofibers 3D composite hydrogel enhances the encapsulated human olfactory mucosa stem cells bioactivity for potential nerve regeneration application.
33278440	1	35	theme	tissue	297:302	arg1	engineering					304:314	tissue engineering	297:314	tissue engineering	297:314	The design of 3D hydrogel constructs to elicit highly controlled cell response is a major field of interest in developing tissue engineering.
33278440	0	36	theme	nerve	143:147	arg1	application					162:172	potential nerve regeneration application	133:172	potential nerve regeneration application	133:172	Alginate-magnetic short nanofibers 3D composite hydrogel enhances the encapsulated human olfactory mucosa stem cells bioactivity for potential nerve regeneration application.
33278440	10	37	theme	short	1476:1480	arg1	nanofibers					1482:1491	the short nanofibers	1472:1491	the short nanofibers	1472:1491	The presence of SPIONs in the short nanofibers can accelerate neural-like differentiation of OE-MSCs rather than the sample without SPIONs.
33278440	6	38	theme	nerve	1075:1079	arg1	tissue					1081:1086	nerve tissue	1075:1086	nerve tissue	1075:1086	The storage modulus of hydrogel without M.SNFs as well as with 1 and 5 mg/mL of M.SNFs were in the range of nerve tissue.
33278440	0	39	theme	nanofibers	24:33	arg1	hydrogel					48:55	Alginate-magnetic short nanofibers 3D composite hydrogel	0:55	Alginate-magnetic short nanofibers 3D composite hydrogel	0:55	Alginate-magnetic short nanofibers 3D composite hydrogel enhances the encapsulated human olfactory mucosa stem cells bioactivity for potential nerve regeneration application.
33278440	0	40	theme	potential	133:141	arg1	application					162:172	potential nerve regeneration application	133:172	potential nerve regeneration application	133:172	Alginate-magnetic short nanofibers 3D composite hydrogel enhances the encapsulated human olfactory mucosa stem cells bioactivity for potential nerve regeneration application.
33278440	7	41	dep	new	1136:1138	arg1	hope					1140:1143	hope	1140:1143	hope	1140:1143	For cell encapsulation, OE-MSCs were used as a new hope for neuronal regeneration due to their neural crest origin.
33278440	3	42	theme	friendly	517:524	arg1	environment					526:536	a cell friendly environment	510:536	a cell friendly environment	510:536	Combining short nanofibers within a hydrogel serves as a promising method to develop a cell friendly environment mimicking the extracellular matrix.
33278440	10	43	from	presence	1450:1457	arg1	nanofibers					1482:1491	the short nanofibers	1472:1491	the short nanofibers	1472:1491	The presence of SPIONs in the short nanofibers can accelerate neural-like differentiation of OE-MSCs rather than the sample without SPIONs.
33278440	8	44	contain	containing	1286:1295	arg2	M.SNFs					1297:1302	M.SNFs	1297:1302	M.SNFs	1297:1302	Resazurin analyses and LIVE/DEAD staining confirmed that the composite hydrogels containing M.SNFs can preserve the cell viability after 7 days.
33278440	8	44	contain	containing	1286:1295	arg1	hydrogels					1276:1284	the composite hydrogels	1262:1284	the composite hydrogels containing M.SNFs	1262:1302	Resazurin analyses and LIVE/DEAD staining confirmed that the composite hydrogels containing M.SNFs can preserve the cell viability after 7 days.
33278440	1	45	theme	controlled	229:238	arg1	response					245:252	highly controlled cell response	222:252	highly controlled cell response	222:252	The design of 3D hydrogel constructs to elicit highly controlled cell response is a major field of interest in developing tissue engineering.
33278440	0	46	theme	composite	38:46	arg1	hydrogel					48:55	Alginate-magnetic short nanofibers 3D composite hydrogel	0:55	Alginate-magnetic short nanofibers 3D composite hydrogel	0:55	Alginate-magnetic short nanofibers 3D composite hydrogel enhances the encapsulated human olfactory mucosa stem cells bioactivity for potential nerve regeneration application.
33278440	0	47	theme	regeneration	149:160	arg1	application					162:172	potential nerve regeneration application	133:172	potential nerve regeneration application	133:172	Alginate-magnetic short nanofibers 3D composite hydrogel enhances the encapsulated human olfactory mucosa stem cells bioactivity for potential nerve regeneration application.
33278440	5	48	theme	iron	802:805	arg1	SPIONs					828:833	SPIONs	828:833	SPIONs	828:833	Wet-electrospun gelatin and superparamagnetic iron oxide nanoparticles (SPIONs) nanocomposite nanofibers were chopped using sonication under optimized conditions and subsequently embedded in alginate hydrogels.
33278440	5	48	theme	iron	802:805	arg1	nanoparticles					813:825	superparamagnetic iron oxide nanoparticles	784:825	superparamagnetic iron oxide nanoparticles (SPIONs)	784:834	Wet-electrospun gelatin and superparamagnetic iron oxide nanoparticles (SPIONs) nanocomposite nanofibers were chopped using sonication under optimized conditions and subsequently embedded in alginate hydrogels.
33278440	5	49	theme	optimized	897:905	arg1	conditions					907:916	optimized conditions	897:916	optimized conditions	897:916	Wet-electrospun gelatin and superparamagnetic iron oxide nanoparticles (SPIONs) nanocomposite nanofibers were chopped using sonication under optimized conditions and subsequently embedded in alginate hydrogels.
33278440	5	50	from	embedded	935:942	arg1	hydrogels					956:964	alginate hydrogels	947:964	alginate hydrogels	947:964	Wet-electrospun gelatin and superparamagnetic iron oxide nanoparticles (SPIONs) nanocomposite nanofibers were chopped using sonication under optimized conditions and subsequently embedded in alginate hydrogels.
33278440	1	51	theme	cell	240:243	arg1	response					245:252	highly controlled cell response	222:252	highly controlled cell response	222:252	The design of 3D hydrogel constructs to elicit highly controlled cell response is a major field of interest in developing tissue engineering.
33278440	7	52	theme	neural	1184:1189	arg1	origin					1197:1202	their neural crest origin	1178:1202	their neural crest origin	1178:1202	For cell encapsulation, OE-MSCs were used as a new hope for neuronal regeneration due to their neural crest origin.
33278440	4	53	theme	cells	725:729	arg1	encapsulation					741:753	olfactory ecto-mesenchymal stem cells (OE-MSCs) encapsulation	693:753	olfactory ecto-mesenchymal stem cells (OE-MSCs) encapsulation	693:753	In this paper, we fabricated alginate hydrogels incorporating different magnetic short nanofibers (M.SNFs) content for olfactory ecto-mesenchymal stem cells (OE-MSCs) encapsulation.
33278440	8	54	theme	composite	1266:1274	arg1	hydrogels					1276:1284	the composite hydrogels	1262:1284	the composite hydrogels containing M.SNFs	1262:1302	Resazurin analyses and LIVE/DEAD staining confirmed that the composite hydrogels containing M.SNFs can preserve the cell viability after 7 days.
33278440	6	55	theme	M.SNFs	1047:1052	arg1	1					1030:1030	1	1030:1030	1	1030:1030	The storage modulus of hydrogel without M.SNFs as well as with 1 and 5 mg/mL of M.SNFs were in the range of nerve tissue.
33278440	6	55	theme	M.SNFs	1047:1052	arg1	M.SNFs					1047:1052	M.SNFs	1047:1052	M.SNFs	1047:1052	The storage modulus of hydrogel without M.SNFs as well as with 1 and 5 mg/mL of M.SNFs were in the range of nerve tissue.
33278440	2	56	theme	alginate	367:374	arg1	hydrogel					376:383	pure alginate hydrogel	362:383	pure alginate hydrogel	362:383	The bioactivity of encapsulated cells inside pure alginate hydrogel is limited by its relatively inertness.
33278440	3	57	theme	short	435:439	arg1	nanofibers					441:450	short nanofibers	435:450	short nanofibers	435:450	Combining short nanofibers within a hydrogel serves as a promising method to develop a cell friendly environment mimicking the extracellular matrix.
33278440	10	58	attach	presence	1450:1457	arg1	nanofibers					1482:1491	the short nanofibers	1472:1491	the short nanofibers	1472:1491	The presence of SPIONs in the short nanofibers can accelerate neural-like differentiation of OE-MSCs rather than the sample without SPIONs.
33278440	10	58	attach	presence	1450:1457	arg2	SPIONs					1462:1467	SPIONs	1462:1467	SPIONs	1462:1467	The presence of SPIONs in the short nanofibers can accelerate neural-like differentiation of OE-MSCs rather than the sample without SPIONs.
33278440	4	59	theme	different	636:644	arg1	content					681:687	different magnetic short nanofibers (M.SNFs) content	636:687	different magnetic short nanofibers (M.SNFs) content for olfactory ecto-mesenchymal stem cells (OE-MSCs) encapsulation	636:753	In this paper, we fabricated alginate hydrogels incorporating different magnetic short nanofibers (M.SNFs) content for olfactory ecto-mesenchymal stem cells (OE-MSCs) encapsulation.
33278440	2	60	theme	pure	362:365	arg1	hydrogel					376:383	pure alginate hydrogel	362:383	pure alginate hydrogel	362:383	The bioactivity of encapsulated cells inside pure alginate hydrogel is limited by its relatively inertness.
33278440	7	61	theme	cell	1093:1096	arg1	encapsulation					1098:1110	cell encapsulation	1093:1110	cell encapsulation	1093:1110	For cell encapsulation, OE-MSCs were used as a new hope for neuronal regeneration due to their neural crest origin.
33278440	8	62	theme	Resazurin	1205:1213	arg1	analyses					1215:1222	Resazurin analyses	1205:1222	Resazurin analyses	1205:1222	Resazurin analyses and LIVE/DEAD staining confirmed that the composite hydrogels containing M.SNFs can preserve the cell viability after 7 days.
33278440	9	63	theme	alginate	1427:1434	arg1	hydrogel					1436:1443	alginate hydrogel	1427:1443	alginate hydrogel	1427:1443	Moreover, the proliferation rate was enhanced in M.SNF/hydrogels compared to alginate hydrogel.
33278440	8	64	theme	cell	1321:1324	arg1	viability					1326:1334	the cell viability	1317:1334	the cell viability	1317:1334	Resazurin analyses and LIVE/DEAD staining confirmed that the composite hydrogels containing M.SNFs can preserve the cell viability after 7 days.
33278440	4	65	theme	short	655:659	arg1	M.SNFs					673:678	M.SNFs	673:678	M.SNFs	673:678	In this paper, we fabricated alginate hydrogels incorporating different magnetic short nanofibers (M.SNFs) content for olfactory ecto-mesenchymal stem cells (OE-MSCs) encapsulation.
33278440	4	65	theme	short	655:659	arg1	nanofibers					661:670	magnetic short nanofibers	646:670	different magnetic short nanofibers (M.SNFs) content for olfactory ecto-mesenchymal stem cells (OE-MSCs) encapsulation	636:753	In this paper, we fabricated alginate hydrogels incorporating different magnetic short nanofibers (M.SNFs) content for olfactory ecto-mesenchymal stem cells (OE-MSCs) encapsulation.
33278440	2	66	theme	cells	349:353	arg1	bioactivity					321:331	The bioactivity	317:331	The bioactivity of encapsulated cells inside pure alginate hydrogel	317:383	The bioactivity of encapsulated cells inside pure alginate hydrogel is limited by its relatively inertness.
33278440	4	67	theme	magnetic	646:653	arg1	M.SNFs					673:678	M.SNFs	673:678	M.SNFs	673:678	In this paper, we fabricated alginate hydrogels incorporating different magnetic short nanofibers (M.SNFs) content for olfactory ecto-mesenchymal stem cells (OE-MSCs) encapsulation.
33278440	4	67	theme	magnetic	646:653	arg1	nanofibers					661:670	magnetic short nanofibers	646:670	different magnetic short nanofibers (M.SNFs) content for olfactory ecto-mesenchymal stem cells (OE-MSCs) encapsulation	636:753	In this paper, we fabricated alginate hydrogels incorporating different magnetic short nanofibers (M.SNFs) content for olfactory ecto-mesenchymal stem cells (OE-MSCs) encapsulation.
33278440	0	68	theme	human	83:87	arg1	cells					111:115	encapsulated human olfactory mucosa stem cells	70:115	the encapsulated human olfactory mucosa stem cells bioactivity for potential nerve regeneration application	66:172	Alginate-magnetic short nanofibers 3D composite hydrogel enhances the encapsulated human olfactory mucosa stem cells bioactivity for potential nerve regeneration application.
33278440	3	69	theme	promising	482:490	arg1	method					492:497	a promising method	480:497	a promising method to develop a cell friendly environment	480:536	Combining short nanofibers within a hydrogel serves as a promising method to develop a cell friendly environment mimicking the extracellular matrix.
33278440	6	70	with	modulus	979:985	arg1	1					1030:1030	1	1030:1030	1	1030:1030	The storage modulus of hydrogel without M.SNFs as well as with 1 and 5 mg/mL of M.SNFs were in the range of nerve tissue.
33278440	6	70	with	modulus	979:985	arg1	M.SNFs					1047:1052	M.SNFs	1047:1052	M.SNFs	1047:1052	The storage modulus of hydrogel without M.SNFs as well as with 1 and 5 mg/mL of M.SNFs were in the range of nerve tissue.
33278440	6	70	with	modulus	979:985	arg1	5 mg/mL					1036:1042	5 mg/mL	1036:1042	5 mg/mL	1036:1042	The storage modulus of hydrogel without M.SNFs as well as with 1 and 5 mg/mL of M.SNFs were in the range of nerve tissue.
33278440	4	71	theme	olfactory	693:701	arg1	OE-MSCs					732:738	OE-MSCs	732:738	OE-MSCs	732:738	In this paper, we fabricated alginate hydrogels incorporating different magnetic short nanofibers (M.SNFs) content for olfactory ecto-mesenchymal stem cells (OE-MSCs) encapsulation.
33278440	4	71	theme	olfactory	693:701	arg1	cells					725:729	olfactory ecto-mesenchymal stem cells	693:729	olfactory ecto-mesenchymal stem cells (OE-MSCs) encapsulation	693:753	In this paper, we fabricated alginate hydrogels incorporating different magnetic short nanofibers (M.SNFs) content for olfactory ecto-mesenchymal stem cells (OE-MSCs) encapsulation.
33278440	6	72	theme	tissue	1081:1086	arg1	range					1066:1070	the range	1062:1070	the range of nerve tissue	1062:1086	The storage modulus of hydrogel without M.SNFs as well as with 1 and 5 mg/mL of M.SNFs were in the range of nerve tissue.
33278440	0	73	theme	encapsulated	70:81	arg1	cells					111:115	encapsulated human olfactory mucosa stem cells	70:115	the encapsulated human olfactory mucosa stem cells bioactivity for potential nerve regeneration application	66:172	Alginate-magnetic short nanofibers 3D composite hydrogel enhances the encapsulated human olfactory mucosa stem cells bioactivity for potential nerve regeneration application.
33278440	4	74	theme	nanofibers	661:670	arg1	content					681:687	different magnetic short nanofibers (M.SNFs) content	636:687	different magnetic short nanofibers (M.SNFs) content for olfactory ecto-mesenchymal stem cells (OE-MSCs) encapsulation	636:753	In this paper, we fabricated alginate hydrogels incorporating different magnetic short nanofibers (M.SNFs) content for olfactory ecto-mesenchymal stem cells (OE-MSCs) encapsulation.
33278440	3	75	theme	extracellular	552:564	arg1	matrix					566:571	the extracellular matrix	548:571	the extracellular matrix	548:571	Combining short nanofibers within a hydrogel serves as a promising method to develop a cell friendly environment mimicking the extracellular matrix.
33278440	1	76	theme	major	259:263	arg1	design					179:184	The design	175:184	The design of 3D hydrogel constructs to elicit highly controlled cell response	175:252	The design of 3D hydrogel constructs to elicit highly controlled cell response is a major field of interest in developing tissue engineering.
33278440	1	76	theme	major	259:263	arg1	field					265:269	a major field	257:269	a major field of interest in developing tissue engineering	257:314	The design of 3D hydrogel constructs to elicit highly controlled cell response is a major field of interest in developing tissue engineering.
32694535	5	0	theme	Such	666:669	arg1	mice					671:674	Such mice	666:674	Such mice	666:674	Such mice were then bred to each other to generate F1 PepT1-/- and WT littermates, which were then bred within their genotype to generate F2, F3, and F4, offspring.
32694535	8	1	dep	observed	1126:1133	arg1	returned					1195:1202	returned	1195:1202	returned gradually over subsequent generations such that, relative to their F4 WT controls, F4 PepT1-/- displayed microbiota composition and colitis-resistant phenotype nearly identical to the founder PepT1-/- mice	1195:1408	Such differences were not observed when comparing F1 PepT1-/- to F1 WT littermates but rather, returned gradually over subsequent generations such that, relative to their F4 WT controls, F4 PepT1-/- displayed microbiota composition and colitis-resistant phenotype nearly identical to the founder PepT1-/- mice.
32694535	8	2	theme	F4	1271:1272	arg1	controls					1277:1284	their F4 WT controls	1265:1284	their F4 WT controls	1265:1284	Such differences were not observed when comparing F1 PepT1-/- to F1 WT littermates but rather, returned gradually over subsequent generations such that, relative to their F4 WT controls, F4 PepT1-/- displayed microbiota composition and colitis-resistant phenotype nearly identical to the founder PepT1-/- mice.
32694535	4	3	theme	PepT1-/-	595:602	arg1	mice					619:622	PepT1-/- and WT founder mice	595:622	PepT1-/- and WT founder mice bred separately for multiple generations	595:663	In this study, we used PepT1-/- and WT founder mice bred separately for multiple generations.
32694535	0	4	theme	multiple	72:79	arg1	generations					81:91	multiple generations	72:91	multiple generations	72:91	Impact of PepT1 deletion on microbiota composition and colitis requires multiple generations.
32694535	11	5	theme	alteration	1772:1781	arg1	impact					1745:1750	impact	1745:1750	impact of a single genetic alteration on microbiota and host phenotype	1745:1814	Rather, impact of a single genetic alteration on microbiota and host phenotype may take generations to manifest.
32694535	0	6	from	Impact	0:5	arg1	colitis					55:61	colitis	55:61	colitis	55:61	Impact of PepT1 deletion on microbiota composition and colitis requires multiple generations.
32694535	0	6	from	Impact	0:5	arg1	composition					39:49	microbiota composition	28:49	microbiota composition	28:49	Impact of PepT1 deletion on microbiota composition and colitis requires multiple generations.
32694535	4	7	theme	WT	608:609	arg1	mice					619:622	PepT1-/- and WT founder mice	595:622	PepT1-/- and WT founder mice bred separately for multiple generations	595:663	In this study, we used PepT1-/- and WT founder mice bred separately for multiple generations.
32694535	5	8	theme	WT	733:734	arg1	littermates					736:746	F1 PepT1-/- and WT littermates	717:746	F1 PepT1-/- and WT littermates	717:746	Such mice were then bred to each other to generate F1 PepT1-/- and WT littermates, which were then bred within their genotype to generate F2, F3, and F4, offspring.
32694535	6	9	theme	PepT1-/-	859:866	arg1	mice					868:871	founder PepT1-/- mice	851:871	founder PepT1-/- mice	851:871	Here we report that founder PepT1-/- mice were, relative to their WT counterparts, resistant to DSS colitis.
32694535	8	10	theme	WT	1274:1275	arg1	controls					1277:1284	their F4 WT controls	1265:1284	their F4 WT controls	1265:1284	Such differences were not observed when comparing F1 PepT1-/- to F1 WT littermates but rather, returned gradually over subsequent generations such that, relative to their F4 WT controls, F4 PepT1-/- displayed microbiota composition and colitis-resistant phenotype nearly identical to the founder PepT1-/- mice.
32694535	11	11	theme	single	1757:1762	arg1	alteration					1772:1781	a single genetic alteration	1755:1781	a single genetic alteration	1755:1781	Rather, impact of a single genetic alteration on microbiota and host phenotype may take generations to manifest.
32694535	1	12	theme	knockout	114:121	arg1	mice					123:126	knockout mice	114:126	knockout mice	114:126	Numerous studies of knockout mice find impacts on microbiota composition that influence host phenotype.
32694535	2	13	theme	WT	273:274	arg1	littermates					276:286	WT littermates	273:286	WT littermates	273:286	However, such differences can vanish when KO mice are compared directly to WT littermates, suggesting these differences do not reflect the genetic deletion per se but microbiota composition drifting over generations.
32694535	10	14	theme	microbiota-dependent	1642:1661	arg1	phenotypes					1663:1672	microbiota-dependent phenotypes	1642:1672	microbiota-dependent phenotypes	1642:1672	Overall, our study indicates that littermate-controlled experiments can be insufficient for assessing microbiota-dependent phenotypes and prevent a full comprehension of genotype-driven phenomena.
32694535	11	15	theme	genetic	1764:1770	arg1	alteration					1772:1781	a single genetic alteration	1755:1781	a single genetic alteration	1755:1781	Rather, impact of a single genetic alteration on microbiota and host phenotype may take generations to manifest.
32694535	1	16	theme	host	182:185	arg1	phenotype					187:195	host phenotype	182:195	host phenotype	182:195	Numerous studies of knockout mice find impacts on microbiota composition that influence host phenotype.
32694535	11	17	theme	microbiota	1786:1795	arg1	phenotype					1806:1814	microbiota and host phenotype	1786:1814	phenotype	1806:1814	Rather, impact of a single genetic alteration on microbiota and host phenotype may take generations to manifest.
32694535	1	18	from	impacts	133:139	arg1	composition					155:165	microbiota composition	144:165	microbiota composition	144:165	Numerous studies of knockout mice find impacts on microbiota composition that influence host phenotype.
32694535	6	19	theme	founder	851:857	arg1	mice					868:871	founder PepT1-/- mice	851:871	founder PepT1-/- mice	851:871	Here we report that founder PepT1-/- mice were, relative to their WT counterparts, resistant to DSS colitis.
32694535	7	20	dep	sufficient	1054:1063	arg1	alterations					976:986	alterations	976:986	alterations	976:986	Such resistance was associated with alterations in gut microbiota, which, when transplanted to germfree mice, was sufficient to transfer resistance to colitis.
32694535	6	21	theme	DSS	927:929	arg1	colitis					931:937	DSS colitis	927:937	DSS colitis	927:937	Here we report that founder PepT1-/- mice were, relative to their WT counterparts, resistant to DSS colitis.
32694535	7	22	theme	germfree	1035:1042	arg1	mice					1044:1047	germfree mice	1035:1047	germfree mice	1035:1047	Such resistance was associated with alterations in gut microbiota, which, when transplanted to germfree mice, was sufficient to transfer resistance to colitis.
32694535	1	23	theme	Numerous	94:101	arg1	studies					103:109	Numerous studies	94:109	Numerous studies of knockout mice	94:126	Numerous studies of knockout mice find impacts on microbiota composition that influence host phenotype.
32694535	2	24	theme	microbiota	365:374	arg1	composition					376:386	microbiota composition	365:386	microbiota composition	365:386	However, such differences can vanish when KO mice are compared directly to WT littermates, suggesting these differences do not reflect the genetic deletion per se but microbiota composition drifting over generations.
32694535	0	25	theme	deletion	16:23	arg1	Impact					0:5	Impact	0:5	Impact of PepT1 deletion on microbiota composition and colitis	0:61	Impact of PepT1 deletion on microbiota composition and colitis requires multiple generations.
32694535	11	26	theme	host	1801:1804	arg1	phenotype					1806:1814	microbiota and host phenotype	1786:1814	phenotype	1806:1814	Rather, impact of a single genetic alteration on microbiota and host phenotype may take generations to manifest.
32694535	10	27	theme	phenomena	1726:1734	arg1	comprehension					1693:1705	a full comprehension	1686:1705	a full comprehension of genotype-driven phenomena	1686:1734	Overall, our study indicates that littermate-controlled experiments can be insufficient for assessing microbiota-dependent phenotypes and prevent a full comprehension of genotype-driven phenomena.
32694535	8	28	theme	colitis-resistant	1336:1352	arg1	phenotype					1354:1362	colitis-resistant phenotype	1336:1362	colitis-resistant phenotype	1336:1362	Such differences were not observed when comparing F1 PepT1-/- to F1 WT littermates but rather, returned gradually over subsequent generations such that, relative to their F4 WT controls, F4 PepT1-/- displayed microbiota composition and colitis-resistant phenotype nearly identical to the founder PepT1-/- mice.
32694535	3	29	theme	transporter	468:478	arg1	PepT1					480:484	di/tri-peptide transporter PepT1	453:484	di/tri-peptide transporter PepT1	453:484	Hence, our hypothesis that absence of di/tri-peptide transporter PepT1 altered microbiota composition resulting in resistance to colitis compelled scrutiny.
32694535	0	30	theme	PepT1	10:14	arg1	deletion					16:23	PepT1 deletion	10:23	PepT1 deletion	10:23	Impact of PepT1 deletion on microbiota composition and colitis requires multiple generations.
32694535	8	31	theme	microbiota	1309:1318	arg1	composition					1320:1330	microbiota composition	1309:1330	microbiota composition	1309:1330	Such differences were not observed when comparing F1 PepT1-/- to F1 WT littermates but rather, returned gradually over subsequent generations such that, relative to their F4 WT controls, F4 PepT1-/- displayed microbiota composition and colitis-resistant phenotype nearly identical to the founder PepT1-/- mice.
32694535	3	32	theme	PepT1	480:484	arg1	absence					442:448	absence	442:448	absence of di/tri-peptide transporter PepT1	442:484	Hence, our hypothesis that absence of di/tri-peptide transporter PepT1 altered microbiota composition resulting in resistance to colitis compelled scrutiny.
32694535	0	33	theme	microbiota	28:37	arg1	composition					39:49	microbiota composition	28:49	microbiota composition	28:49	Impact of PepT1 deletion on microbiota composition and colitis requires multiple generations.
32694535	10	34	theme	full	1688:1691	arg1	comprehension					1693:1705	a full comprehension	1686:1705	a full comprehension of genotype-driven phenomena	1686:1734	Overall, our study indicates that littermate-controlled experiments can be insufficient for assessing microbiota-dependent phenotypes and prevent a full comprehension of genotype-driven phenomena.
32694535	5	35	theme	F1	717:718	arg1	littermates					736:746	F1 PepT1-/- and WT littermates	717:746	F1 PepT1-/- and WT littermates	717:746	Such mice were then bred to each other to generate F1 PepT1-/- and WT littermates, which were then bred within their genotype to generate F2, F3, and F4, offspring.
32694535	8	36	theme	PepT1-/-	1396:1403	arg1	mice					1405:1408	the founder PepT1-/- mice	1384:1408	the founder PepT1-/- mice	1384:1408	Such differences were not observed when comparing F1 PepT1-/- to F1 WT littermates but rather, returned gradually over subsequent generations such that, relative to their F4 WT controls, F4 PepT1-/- displayed microbiota composition and colitis-resistant phenotype nearly identical to the founder PepT1-/- mice.
32694535	2	37	theme	KO	240:241	arg1	mice					243:246	KO mice	240:246	KO mice	240:246	However, such differences can vanish when KO mice are compared directly to WT littermates, suggesting these differences do not reflect the genetic deletion per se but microbiota composition drifting over generations.
32694535	8	38	theme	subsequent	1219:1228	arg1	generations					1230:1240	subsequent generations	1219:1240	subsequent generations such that, relative to their F4 WT controls, F4 PepT1-/- displayed microbiota composition and colitis-resistant phenotype nearly identical to the founder PepT1-/- mice	1219:1408	Such differences were not observed when comparing F1 PepT1-/- to F1 WT littermates but rather, returned gradually over subsequent generations such that, relative to their F4 WT controls, F4 PepT1-/- displayed microbiota composition and colitis-resistant phenotype nearly identical to the founder PepT1-/- mice.
32694535	10	39	theme	littermate-controlled	1574:1594	arg1	experiments					1596:1606	littermate-controlled experiments	1574:1606	littermate-controlled experiments	1574:1606	Overall, our study indicates that littermate-controlled experiments can be insufficient for assessing microbiota-dependent phenotypes and prevent a full comprehension of genotype-driven phenomena.
32694535	5	40	theme	PepT1-/-	720:727	arg1	littermates					736:746	F1 PepT1-/- and WT littermates	717:746	F1 PepT1-/- and WT littermates	717:746	Such mice were then bred to each other to generate F1 PepT1-/- and WT littermates, which were then bred within their genotype to generate F2, F3, and F4, offspring.
32694535	8	41	theme	F1	1150:1151	arg1	PepT1-/-					1153:1160	F1 PepT1-/-	1150:1160	F1 PepT1-/-	1150:1160	Such differences were not observed when comparing F1 PepT1-/- to F1 WT littermates but rather, returned gradually over subsequent generations such that, relative to their F4 WT controls, F4 PepT1-/- displayed microbiota composition and colitis-resistant phenotype nearly identical to the founder PepT1-/- mice.
32694535	3	42	theme	di/tri-peptide	453:466	arg1	PepT1					480:484	di/tri-peptide transporter PepT1	453:484	di/tri-peptide transporter PepT1	453:484	Hence, our hypothesis that absence of di/tri-peptide transporter PepT1 altered microbiota composition resulting in resistance to colitis compelled scrutiny.
32694535	8	43	theme	WT	1168:1169	arg1	littermates					1171:1181	F1 WT littermates	1165:1181	F1 WT littermates	1165:1181	Such differences were not observed when comparing F1 PepT1-/- to F1 WT littermates but rather, returned gradually over subsequent generations such that, relative to their F4 WT controls, F4 PepT1-/- displayed microbiota composition and colitis-resistant phenotype nearly identical to the founder PepT1-/- mice.
32694535	6	44	theme	WT	897:898	arg1	counterparts					900:911	their WT counterparts	891:911	their WT counterparts	891:911	Here we report that founder PepT1-/- mice were, relative to their WT counterparts, resistant to DSS colitis.
32694535	11	45	from	impact	1745:1750	arg1	phenotype					1806:1814	microbiota and host phenotype	1786:1814	phenotype	1806:1814	Rather, impact of a single genetic alteration on microbiota and host phenotype may take generations to manifest.
32694535	2	46	theme	genetic	337:343	arg1	deletion					345:352	the genetic deletion	333:352	the genetic deletion	333:352	However, such differences can vanish when KO mice are compared directly to WT littermates, suggesting these differences do not reflect the genetic deletion per se but microbiota composition drifting over generations.
32694535	8	47	theme	F1	1165:1166	arg1	littermates					1171:1181	F1 WT littermates	1165:1181	F1 WT littermates	1165:1181	Such differences were not observed when comparing F1 PepT1-/- to F1 WT littermates but rather, returned gradually over subsequent generations such that, relative to their F4 WT controls, F4 PepT1-/- displayed microbiota composition and colitis-resistant phenotype nearly identical to the founder PepT1-/- mice.
32694535	1	48	theme	mice	123:126	arg1	studies					103:109	Numerous studies	94:109	Numerous studies of knockout mice	94:126	Numerous studies of knockout mice find impacts on microbiota composition that influence host phenotype.
32694535	4	49	used	used	590:593	arg2	we					587:588	we	587:588	we	587:588	In this study, we used PepT1-/- and WT founder mice bred separately for multiple generations.
32694535	2	50	theme	such	207:210	arg1	differences					212:222	such differences	207:222	such differences	207:222	However, such differences can vanish when KO mice are compared directly to WT littermates, suggesting these differences do not reflect the genetic deletion per se but microbiota composition drifting over generations.
32694535	10	51	theme	genotype-driven	1710:1724	arg1	phenomena					1726:1734	genotype-driven phenomena	1710:1734	genotype-driven phenomena	1710:1734	Overall, our study indicates that littermate-controlled experiments can be insufficient for assessing microbiota-dependent phenotypes and prevent a full comprehension of genotype-driven phenomena.
32694535	7	52	theme	gut	991:993	arg1	microbiota					995:1004	gut microbiota	991:1004	gut microbiota	991:1004	Such resistance was associated with alterations in gut microbiota, which, when transplanted to germfree mice, was sufficient to transfer resistance to colitis.
32694535	8	53	theme	identical	1371:1379	arg1	composition					1320:1330	microbiota composition	1309:1330	microbiota composition	1309:1330	Such differences were not observed when comparing F1 PepT1-/- to F1 WT littermates but rather, returned gradually over subsequent generations such that, relative to their F4 WT controls, F4 PepT1-/- displayed microbiota composition and colitis-resistant phenotype nearly identical to the founder PepT1-/- mice.
32694535	7	54	theme	Such	940:943	arg1	resistance					945:954	Such resistance	940:954	Such resistance	940:954	Such resistance was associated with alterations in gut microbiota, which, when transplanted to germfree mice, was sufficient to transfer resistance to colitis.
32694535	3	55	theme	microbiota	494:503	arg1	composition					505:515	microbiota composition	494:515	microbiota composition resulting in resistance to colitis	494:550	Hence, our hypothesis that absence of di/tri-peptide transporter PepT1 altered microbiota composition resulting in resistance to colitis compelled scrutiny.
32694535	4	56	theme	founder	611:617	arg1	mice					619:622	PepT1-/- and WT founder mice	595:622	PepT1-/- and WT founder mice bred separately for multiple generations	595:663	In this study, we used PepT1-/- and WT founder mice bred separately for multiple generations.
32694535	7	57	from	alterations	976:986	arg1	microbiota					995:1004	gut microbiota	991:1004	gut microbiota	991:1004	Such resistance was associated with alterations in gut microbiota, which, when transplanted to germfree mice, was sufficient to transfer resistance to colitis.
32694535	8	58	theme	founder	1388:1394	arg1	mice					1405:1408	the founder PepT1-/- mice	1384:1408	the founder PepT1-/- mice	1384:1408	Such differences were not observed when comparing F1 PepT1-/- to F1 WT littermates but rather, returned gradually over subsequent generations such that, relative to their F4 WT controls, F4 PepT1-/- displayed microbiota composition and colitis-resistant phenotype nearly identical to the founder PepT1-/- mice.
32694535	4	59	theme	multiple	644:651	arg1	generations					653:663	multiple generations	644:663	multiple generations	644:663	In this study, we used PepT1-/- and WT founder mice bred separately for multiple generations.
32694535	9	60	theme	microbiota	1465:1474	arg1	composition					1476:1486	microbiota composition	1465:1486	microbiota composition	1465:1486	Our findings indicate a role for PepT1 in influencing microbiota composition and, consequently, proneness to colitis and cancer.
32694535	1	61	theme	microbiota	144:153	arg1	composition					155:165	microbiota composition	144:165	microbiota composition	144:165	Numerous studies of knockout mice find impacts on microbiota composition that influence host phenotype.
32694535	8	62	theme	Such	1100:1103	arg1	differences					1105:1115	Such differences	1100:1115	Such differences	1100:1115	Such differences were not observed when comparing F1 PepT1-/- to F1 WT littermates but rather, returned gradually over subsequent generations such that, relative to their F4 WT controls, F4 PepT1-/- displayed microbiota composition and colitis-resistant phenotype nearly identical to the founder PepT1-/- mice.
34502243	2	0	theme	in	317:318	arg1	synthesis					325:333	in situ synthesis	317:333	in situ synthesis	317:333	This study aimed to produce scaffolds based on polyvinyl alcohol (PVA) and hyaluronic acid (HA) with hydroxyapatite (HAp) incorporated in two different ways, by in situ synthesis and physical mixing of pre-prepared HAp.
34502243	2	1	dep	in	317:318	arg1	situ					320:323	situ	320:323	situ	320:323	This study aimed to produce scaffolds based on polyvinyl alcohol (PVA) and hyaluronic acid (HA) with hydroxyapatite (HAp) incorporated in two different ways, by in situ synthesis and physical mixing of pre-prepared HAp.
34502243	4	2	theme	cells	505:509	arg1	proliferation					466:478	The proliferation	462:478	The proliferation of human osteoblast-like cells MG-63	462:515	The proliferation of human osteoblast-like cells MG-63 proved to be better in the scaffolds with in situ synthesized HAp compared to those with physically mixed pre-prepared HAp.
34502243	1	3	theme	natural	122:128	arg1	behavior					130:137	natural behavior	122:137	natural behavior of hard tissues	122:153	Bone tissue engineering tries to simulate natural behavior of hard tissues.
34502243	1	4	theme	Bone	80:83	arg1	engineering					92:102	Bone tissue engineering	80:102	Bone tissue engineering	80:102	Bone tissue engineering tries to simulate natural behavior of hard tissues.
34502243	5	5	theme	p	798:798	arg1	days					792:795	the following days	778:795	the following days (p < 0.001)	778:807	For scaffolds with PVA/HA/HAp ratio 3:1:2, there was significantly higher initial adhesion (p = 0.0440), as well as the proliferation in the following days (p < 0.001).
34502243	5	5	theme	p	798:798	arg1	<					800:800	p < 0.001	798:806	p < 0.001	798:806	For scaffolds with PVA/HA/HAp ratio 3:1:2, there was significantly higher initial adhesion (p = 0.0440), as well as the proliferation in the following days (p < 0.001).
34502243	4	6	theme	mixed	617:621	arg1	HAp					636:638	physically mixed pre-prepared HAp	606:638	physically mixed pre-prepared HAp	606:638	The proliferation of human osteoblast-like cells MG-63 proved to be better in the scaffolds with in situ synthesized HAp compared to those with physically mixed pre-prepared HAp.
34502243	1	7	theme	tissue	85:90	arg1	engineering					92:102	Bone tissue engineering	80:102	Bone tissue engineering	80:102	Bone tissue engineering tries to simulate natural behavior of hard tissues.
34502243	5	8	dep	higher	708:713	arg1	adhesion					723:730	initial adhesion	715:730	initial adhesion	715:730	For scaffolds with PVA/HA/HAp ratio 3:1:2, there was significantly higher initial adhesion (p = 0.0440), as well as the proliferation in the following days (p < 0.001).
34502243	5	8	dep	higher	708:713	arg1	=					735:735	=	735:735	=	735:735	For scaffolds with PVA/HA/HAp ratio 3:1:2, there was significantly higher initial adhesion (p = 0.0440), as well as the proliferation in the following days (p < 0.001).
34502243	2	9	theme	physical	339:346	arg1	mixing					348:353	physical mixing	339:353	physical mixing of pre-prepared HAp	339:373	This study aimed to produce scaffolds based on polyvinyl alcohol (PVA) and hyaluronic acid (HA) with hydroxyapatite (HAp) incorporated in two different ways, by in situ synthesis and physical mixing of pre-prepared HAp.
34502243	0	10	theme	In	0:1	arg1	Synthesis					23:31	In Situ Hydroxyapatite Synthesis	0:31	In Situ Hydroxyapatite Synthesis	0:31	In Situ Hydroxyapatite Synthesis Enhances Biocompatibility of PVA/HA Hydrogels.
34502243	3	11	dep	In	376:377	arg1	situ					379:382	situ	379:382	situ	379:382	In situ synthesis resulted in calcium deficient form of HAp with lower crystallinity.
34502243	4	12	theme	pre-prepared	623:634	arg1	HAp					636:638	physically mixed pre-prepared HAp	606:638	physically mixed pre-prepared HAp	606:638	The proliferation of human osteoblast-like cells MG-63 proved to be better in the scaffolds with in situ synthesized HAp compared to those with physically mixed pre-prepared HAp.
34502243	5	13	theme	PVA/HA/HAp	660:669	arg1	3:1:2					677:681	PVA/HA/HAp ratio 3:1:2	660:681	PVA/HA/HAp ratio 3:1:2	660:681	For scaffolds with PVA/HA/HAp ratio 3:1:2, there was significantly higher initial adhesion (p = 0.0440), as well as the proliferation in the following days (p < 0.001).
34502243	0	14	theme	Hydroxyapatite	8:21	arg1	Synthesis					23:31	In Situ Hydroxyapatite Synthesis	0:31	In Situ Hydroxyapatite Synthesis	0:31	In Situ Hydroxyapatite Synthesis Enhances Biocompatibility of PVA/HA Hydrogels.
34502243	6	15	theme	scaffold	869:876	arg1	properties					851:860	the properties	847:860	the properties of the scaffold	847:876	It seemed to be advantageous improve the properties of the scaffold by in situ synthesizing of HAp directly in the organic matrix.
34502243	5	16	theme	ratio	671:675	arg1	3:1:2					677:681	PVA/HA/HAp ratio 3:1:2	660:681	PVA/HA/HAp ratio 3:1:2	660:681	For scaffolds with PVA/HA/HAp ratio 3:1:2, there was significantly higher initial adhesion (p = 0.0440), as well as the proliferation in the following days (p < 0.001).
34502243	3	17	with	form	424:427	arg1	crystallinity					447:459	lower crystallinity	441:459	lower crystallinity	441:459	In situ synthesis resulted in calcium deficient form of HAp with lower crystallinity.
34502243	2	18	theme	HAp	371:373	arg1	mixing					348:353	physical mixing	339:353	physical mixing of pre-prepared HAp	339:373	This study aimed to produce scaffolds based on polyvinyl alcohol (PVA) and hyaluronic acid (HA) with hydroxyapatite (HAp) incorporated in two different ways, by in situ synthesis and physical mixing of pre-prepared HAp.
34502243	2	18	theme	HAp	371:373	arg1	synthesis					325:333	in situ synthesis	317:333	in situ synthesis	317:333	This study aimed to produce scaffolds based on polyvinyl alcohol (PVA) and hyaluronic acid (HA) with hydroxyapatite (HAp) incorporated in two different ways, by in situ synthesis and physical mixing of pre-prepared HAp.
34502243	3	19	theme	In	376:377	arg1	synthesis					384:392	In situ synthesis	376:392	In situ synthesis	376:392	In situ synthesis resulted in calcium deficient form of HAp with lower crystallinity.
34502243	4	20	theme	osteoblast-like	489:503	arg1	cells					505:509	human osteoblast-like cells	483:509	human osteoblast-like cells MG-63	483:515	The proliferation of human osteoblast-like cells MG-63 proved to be better in the scaffolds with in situ synthesized HAp compared to those with physically mixed pre-prepared HAp.
34502243	5	21	theme	following	782:790	arg1	days					792:795	the following days	778:795	the following days (p < 0.001)	778:807	For scaffolds with PVA/HA/HAp ratio 3:1:2, there was significantly higher initial adhesion (p = 0.0440), as well as the proliferation in the following days (p < 0.001).
34502243	5	21	theme	following	782:790	arg1	<					800:800	p < 0.001	798:806	p < 0.001	798:806	For scaffolds with PVA/HA/HAp ratio 3:1:2, there was significantly higher initial adhesion (p = 0.0440), as well as the proliferation in the following days (p < 0.001).
34502243	3	22	theme	deficient	414:422	arg1	form					424:427	calcium deficient form	406:427	calcium deficient form of HAp with lower crystallinity	406:459	In situ synthesis resulted in calcium deficient form of HAp with lower crystallinity.
34502243	0	23	theme	PVA/HA	62:67	arg1	Hydrogels					69:77	PVA/HA Hydrogels	62:77	PVA/HA Hydrogels	62:77	In Situ Hydroxyapatite Synthesis Enhances Biocompatibility of PVA/HA Hydrogels.
34502243	4	24	theme	human	483:487	arg1	cells					505:509	human osteoblast-like cells	483:509	human osteoblast-like cells MG-63	483:515	The proliferation of human osteoblast-like cells MG-63 proved to be better in the scaffolds with in situ synthesized HAp compared to those with physically mixed pre-prepared HAp.
34502243	3	25	theme	lower	441:445	arg1	crystallinity					447:459	lower crystallinity	441:459	lower crystallinity	441:459	In situ synthesis resulted in calcium deficient form of HAp with lower crystallinity.
34502243	3	26	theme	HAp	432:434	arg1	form					424:427	calcium deficient form	406:427	calcium deficient form of HAp with lower crystallinity	406:459	In situ synthesis resulted in calcium deficient form of HAp with lower crystallinity.
34502243	5	27	with	scaffolds	645:653	arg1	3:1:2					677:681	PVA/HA/HAp ratio 3:1:2	660:681	PVA/HA/HAp ratio 3:1:2	660:681	For scaffolds with PVA/HA/HAp ratio 3:1:2, there was significantly higher initial adhesion (p = 0.0440), as well as the proliferation in the following days (p < 0.001).
34502243	5	28	from	proliferation	761:773	arg1	days					792:795	the following days	778:795	the following days (p < 0.001)	778:807	For scaffolds with PVA/HA/HAp ratio 3:1:2, there was significantly higher initial adhesion (p = 0.0440), as well as the proliferation in the following days (p < 0.001).
34502243	5	28	from	proliferation	761:773	arg1	<					800:800	p < 0.001	798:806	p < 0.001	798:806	For scaffolds with PVA/HA/HAp ratio 3:1:2, there was significantly higher initial adhesion (p = 0.0440), as well as the proliferation in the following days (p < 0.001).
34502243	0	29	dep	In	0:1	arg1	Situ					3:6	Situ	3:6	Situ	3:6	In Situ Hydroxyapatite Synthesis Enhances Biocompatibility of PVA/HA Hydrogels.
34502243	2	30	theme	polyvinyl	203:211	arg1	PVA					222:224	PVA	222:224	PVA	222:224	This study aimed to produce scaffolds based on polyvinyl alcohol (PVA) and hyaluronic acid (HA) with hydroxyapatite (HAp) incorporated in two different ways, by in situ synthesis and physical mixing of pre-prepared HAp.
34502243	2	30	theme	polyvinyl	203:211	arg1	alcohol					213:219	polyvinyl alcohol	203:219	polyvinyl alcohol (PVA)	203:225	This study aimed to produce scaffolds based on polyvinyl alcohol (PVA) and hyaluronic acid (HA) with hydroxyapatite (HAp) incorporated in two different ways, by in situ synthesis and physical mixing of pre-prepared HAp.
34502243	2	31	theme	different	298:306	arg1	ways					308:311	two different ways	294:311	two different ways	294:311	This study aimed to produce scaffolds based on polyvinyl alcohol (PVA) and hyaluronic acid (HA) with hydroxyapatite (HAp) incorporated in two different ways, by in situ synthesis and physical mixing of pre-prepared HAp.
34502243	6	32	theme	organic	925:931	arg1	matrix					933:938	the organic matrix	921:938	the organic matrix	921:938	It seemed to be advantageous improve the properties of the scaffold by in situ synthesizing of HAp directly in the organic matrix.
34502243	4	33	with	scaffolds	544:552	arg1	HAp					579:581	in situ synthesized HAp	559:581	in situ synthesized HAp	559:581	The proliferation of human osteoblast-like cells MG-63 proved to be better in the scaffolds with in situ synthesized HAp compared to those with physically mixed pre-prepared HAp.
34502243	1	34	theme	hard	142:145	arg1	tissues					147:153	hard tissues	142:153	hard tissues	142:153	Bone tissue engineering tries to simulate natural behavior of hard tissues.
34502243	2	35	with	scaffolds	184:192	arg1	hydroxyapatite					257:270	hydroxyapatite	257:270	hydroxyapatite (HAp) incorporated in two different ways	257:311	This study aimed to produce scaffolds based on polyvinyl alcohol (PVA) and hyaluronic acid (HA) with hydroxyapatite (HAp) incorporated in two different ways, by in situ synthesis and physical mixing of pre-prepared HAp.
34502243	2	35	with	scaffolds	184:192	arg1	HAp					273:275	HAp	273:275	HAp	273:275	This study aimed to produce scaffolds based on polyvinyl alcohol (PVA) and hyaluronic acid (HA) with hydroxyapatite (HAp) incorporated in two different ways, by in situ synthesis and physical mixing of pre-prepared HAp.
34502243	5	36	theme	initial	715:721	arg1	adhesion					723:730	initial adhesion	715:730	initial adhesion	715:730	For scaffolds with PVA/HA/HAp ratio 3:1:2, there was significantly higher initial adhesion (p = 0.0440), as well as the proliferation in the following days (p < 0.001).
34502243	2	37	theme	pre-prepared	358:369	arg1	HAp					371:373	pre-prepared HAp	358:373	pre-prepared HAp	358:373	This study aimed to produce scaffolds based on polyvinyl alcohol (PVA) and hyaluronic acid (HA) with hydroxyapatite (HAp) incorporated in two different ways, by in situ synthesis and physical mixing of pre-prepared HAp.
34502243	1	38	theme	tissues	147:153	arg1	behavior					130:137	natural behavior	122:137	natural behavior of hard tissues	122:153	Bone tissue engineering tries to simulate natural behavior of hard tissues.
34502243	0	39	theme	Hydrogels	69:77	arg1	Biocompatibility					42:57	Biocompatibility	42:57	Biocompatibility of PVA/HA Hydrogels	42:77	In Situ Hydroxyapatite Synthesis Enhances Biocompatibility of PVA/HA Hydrogels.
34502243	2	40	theme	hyaluronic	231:240	arg1	acid					242:245	hyaluronic acid	231:245	hyaluronic acid (HA)	231:250	This study aimed to produce scaffolds based on polyvinyl alcohol (PVA) and hyaluronic acid (HA) with hydroxyapatite (HAp) incorporated in two different ways, by in situ synthesis and physical mixing of pre-prepared HAp.
34502243	2	40	theme	hyaluronic	231:240	arg1	HA					248:249	HA	248:249	HA	248:249	This study aimed to produce scaffolds based on polyvinyl alcohol (PVA) and hyaluronic acid (HA) with hydroxyapatite (HAp) incorporated in two different ways, by in situ synthesis and physical mixing of pre-prepared HAp.
34502243	4	41	theme	synthesized	567:577	arg1	HAp					579:581	in situ synthesized HAp	559:581	in situ synthesized HAp	559:581	The proliferation of human osteoblast-like cells MG-63 proved to be better in the scaffolds with in situ synthesized HAp compared to those with physically mixed pre-prepared HAp.
32113757	17	0	theme	limited	2153:2159	arg1	numbers					2168:2174	the limited animal numbers	2149:2174	the limited animal numbers in the present experiment	2149:2200	Despite the limited animal numbers in the present experiment, there was a significant decrease in the total number of health events (mainly respiratory) requiring therapeutic intervention and in the total number of therapeutic interventions in calves fed high-fat CMR.
32113757	18	1	from	difference	2525:2534	arg1	content					2546:2552	energy content	2539:2552	energy content of the CMR	2539:2563	Calves appeared to consume CMR based on energy content, with a difference in ad libitum intake proportional to the difference in energy content of the CMR, maintaining equal body weight gain and solid feed intake.
32113757	12	2	theme	dietary	1568:1574	arg1	components					1576:1585	all dietary components	1564:1585	all dietary components	1564:1585	Measurements included the intake of all dietary components, body weight gain, and a selection of blood traits.
32113757	3	3	from	lactose	458:464	arg1	CMR					477:479	CMR	477:479	CMR	477:479	This study was designed to determine the effect of exchanging lactose for fat in CMR on voluntary feed intake and growth performance.
32113757	2	4	theme	young	307:311	arg1	calves					313:318	young calves	307:318	young calves provided with ad libitum milk and solid feeds	307:364	Additionally, feed intake regulation in young calves provided with ad libitum milk and solid feeds is insufficiently understood.
32113757	9	5	theme	ad	1189:1190	arg1	access					1200:1205	ad libitum access	1189:1205	ad libitum access to their assigned CMR treatments, starter feed, chopped wheat straw, and water	1189:1284	In period 2, calves were group-housed with 8 calves per pen and received ad libitum access to their assigned CMR treatments, starter feed, chopped wheat straw, and water.
32113757	3	6	theme	feed	494:497	arg1	intake					499:504	voluntary feed intake	484:504	voluntary feed intake	484:504	This study was designed to determine the effect of exchanging lactose for fat in CMR on voluntary feed intake and growth performance.
32113757	1	7	theme	milk	233:236	arg1	CMR					248:250	CMR	248:250	CMR	248:250	The recent trend in the dairy industry toward ad libitum feeding of young calves merits reconsideration of calf milk replacer (CMR) formulations.
32113757	1	7	theme	milk	233:236	arg1	replacer					238:245	calf milk replacer	228:245	calf milk replacer (CMR) formulations	228:264	The recent trend in the dairy industry toward ad libitum feeding of young calves merits reconsideration of calf milk replacer (CMR) formulations.
32113757	8	8	theme	CMR	1001:1003	arg1	treatments					1005:1014	their assigned CMR treatments	986:1014	their assigned CMR treatments	986:1014	In period 1, until 14 ± 1.7 d of age, calves were individually housed, restricted-fed their assigned CMR treatments at 2.5 to 3 L twice daily, and provided with unlimited access to water, chopped straw, and starter.
32113757	2	9	from	regulation	293:302	arg1	calves					313:318	young calves	307:318	young calves provided with ad libitum milk and solid feeds	307:364	Additionally, feed intake regulation in young calves provided with ad libitum milk and solid feeds is insufficiently understood.
32113757	2	10	theme	libitum	337:343	arg1	milk					345:348	ad libitum milk	334:348	ad libitum milk	334:348	Additionally, feed intake regulation in young calves provided with ad libitum milk and solid feeds is insufficiently understood.
32113757	4	11	theme	different	599:607	arg1	contents					616:623	different energy contents	599:623	different energy contents per kilogram of CMR	599:643	Lactose was exchanged for fat on a weight/weight basis, resulting in different energy contents per kilogram of CMR.
32113757	16	12	theme	plasma	2106:2111	arg1	glucose					2113:2119	plasma glucose	2106:2119	plasma glucose	2106:2119	High-fat CMR increased plasma phosphate, nonesterified fatty acids, triglycerides, and bilirubin, whereas plasma glucose remained unchanged.
32113757	17	13	theme	therapeutic	2356:2366	arg1	interventions					2368:2380	therapeutic interventions	2356:2380	therapeutic interventions in calves fed high-fat CMR	2356:2407	Despite the limited animal numbers in the present experiment, there was a significant decrease in the total number of health events (mainly respiratory) requiring therapeutic intervention and in the total number of therapeutic interventions in calves fed high-fat CMR.
32113757	10	14	theme	age	1326:1328	arg1	d					1321:1321	63 d	1318:1321	63 d of age	1318:1328	During period 3, from 43 until 63 d of age, calves were weaned by restricting CMR allowance in 2 steps, maintaining access to all other feeds.
32113757	9	15	theme	CMR	1225:1227	arg1	treatments					1229:1238	their assigned CMR treatments	1210:1238	their assigned CMR treatments	1210:1238	In period 2, calves were group-housed with 8 calves per pen and received ad libitum access to their assigned CMR treatments, starter feed, chopped wheat straw, and water.
32113757	12	16	theme	traits	1631:1636	arg1	intake					1554:1559	the intake	1550:1559	the intake of all dietary components	1550:1585	Measurements included the intake of all dietary components, body weight gain, and a selection of blood traits.
32113757	12	16	theme	traits	1631:1636	arg1	gain					1600:1603	body weight gain	1588:1603	body weight gain	1588:1603	Measurements included the intake of all dietary components, body weight gain, and a selection of blood traits.
32113757	12	16	theme	traits	1631:1636	arg1	selection					1612:1620	a selection	1610:1620	a selection of blood traits	1610:1636	Measurements included the intake of all dietary components, body weight gain, and a selection of blood traits.
32113757	17	17	from	interventions	2368:2380	arg1	calves					2385:2390	calves	2385:2390	calves fed high-fat CMR	2385:2407	Despite the limited animal numbers in the present experiment, there was a significant decrease in the total number of health events (mainly respiratory) requiring therapeutic intervention and in the total number of therapeutic interventions in calves fed high-fat CMR.
32113757	17	18	theme	events	2266:2271	arg1	number					2249:2254	the total number	2239:2254	the total number of health events (mainly respiratory) requiring therapeutic intervention	2239:2327	Despite the limited animal numbers in the present experiment, there was a significant decrease in the total number of health events (mainly respiratory) requiring therapeutic intervention and in the total number of therapeutic interventions in calves fed high-fat CMR.
32113757	18	19	from	difference	2473:2482	arg1	intake					2498:2503	ad libitum intake	2487:2503	ad libitum intake proportional to the difference in energy content of the CMR	2487:2563	Calves appeared to consume CMR based on energy content, with a difference in ad libitum intake proportional to the difference in energy content of the CMR, maintaining equal body weight gain and solid feed intake.
32113757	13	20	theme	energy	1756:1761	arg1	intake					1763:1768	total calculated metabolizable energy intake	1725:1768	total calculated metabolizable energy intake	1725:1768	Increasing fat content at the expense of lactose decreased CMR intake by 10%, whereas total calculated metabolizable energy intake and growth remained equal between treatments.
32113757	1	21	theme	ad	167:168	arg1	feeding					178:184	ad libitum feeding	167:184	ad libitum feeding of young calves	167:200	The recent trend in the dairy industry toward ad libitum feeding of young calves merits reconsideration of calf milk replacer (CMR) formulations.
32113757	0	22	theme	dairy	107:111	arg1	calves					113:118	dairy calves	107:118	dairy calves	107:118	Effects of exchanging lactose for fat in milk replacer on ad libitum feed intake and growth performance in dairy calves.
32113757	5	23	theme	weight	714:719	arg1	kg					703:704	47.6 ± 0.83 kg	691:704	47.6 ± 0.83 kg of body weight	691:719	Thirty-two male calves (1.7 ± 0.12 d of age, 47.6 ± 0.83 kg of body weight) were assigned to 1 of 16 blocks based on arrival date.
32113757	5	23	theme	weight	714:719	arg1	d					681:681	1.7 ± 0.12 d	670:681	1.7 ± 0.12 d of age	670:688	Thirty-two male calves (1.7 ± 0.12 d of age, 47.6 ± 0.83 kg of body weight) were assigned to 1 of 16 blocks based on arrival date.
32113757	0	24	from	replacer	46:53	arg1	Effects					0:6	Effects	0:6	Effects of exchanging lactose for fat in milk replacer on ad libitum feed intake and growth performance in dairy calves.	0:119	Effects of exchanging lactose for fat in milk replacer on ad libitum feed intake and growth performance in dairy calves.
32113757	8	25	theme	chopped	1088:1094	arg1	straw					1096:1100	chopped straw	1088:1100	chopped straw	1088:1100	In period 1, until 14 ± 1.7 d of age, calves were individually housed, restricted-fed their assigned CMR treatments at 2.5 to 3 L twice daily, and provided with unlimited access to water, chopped straw, and starter.
32113757	11	26	theme	77	1504:1505	arg1	d					1507:1507	77 d	1504:1507	77 d of age (period 4)	1504:1525	All calves were completely weaned at d 64 of age and were monitored until 77 d of age (period 4).
32113757	11	26	theme	77	1504:1505	arg1	period					1517:1522	period 4	1517:1524	period 4	1517:1524	All calves were completely weaned at d 64 of age and were monitored until 77 d of age (period 4).
32113757	15	27	theme	energy	1984:1989	arg1	content					1991:1997	energy content	1984:1997	energy content	1984:1997	These data indicate that calves fed ad libitum regulate their CMR intake based on energy content.
32113757	9	28	theme	chopped	1255:1261	arg1	straw					1269:1273	chopped wheat straw	1255:1273	chopped wheat straw	1255:1273	In period 2, calves were group-housed with 8 calves per pen and received ad libitum access to their assigned CMR treatments, starter feed, chopped wheat straw, and water.
32113757	17	29	theme	therapeutic	2304:2314	arg1	intervention					2316:2327	therapeutic intervention	2304:2327	therapeutic intervention	2304:2327	Despite the limited animal numbers in the present experiment, there was a significant decrease in the total number of health events (mainly respiratory) requiring therapeutic intervention and in the total number of therapeutic interventions in calves fed high-fat CMR.
32113757	13	30	theme	calculated	1731:1740	arg1	intake					1763:1768	total calculated metabolizable energy intake	1725:1768	total calculated metabolizable energy intake	1725:1768	Increasing fat content at the expense of lactose decreased CMR intake by 10%, whereas total calculated metabolizable energy intake and growth remained equal between treatments.
32113757	1	31	theme	calf	228:231	arg1	CMR					248:250	CMR	248:250	CMR	248:250	The recent trend in the dairy industry toward ad libitum feeding of young calves merits reconsideration of calf milk replacer (CMR) formulations.
32113757	1	31	theme	calf	228:231	arg1	replacer					238:245	calf milk replacer	228:245	calf milk replacer (CMR) formulations	228:264	The recent trend in the dairy industry toward ad libitum feeding of young calves merits reconsideration of calf milk replacer (CMR) formulations.
32113757	5	32	theme	arrival	763:769	arg1	date					771:774	arrival date	763:774	arrival date	763:774	Thirty-two male calves (1.7 ± 0.12 d of age, 47.6 ± 0.83 kg of body weight) were assigned to 1 of 16 blocks based on arrival date.
32113757	17	33	theme	health	2259:2264	arg1	events					2266:2271	health events	2259:2271	health events (mainly respiratory) requiring therapeutic intervention	2259:2327	Despite the limited animal numbers in the present experiment, there was a significant decrease in the total number of health events (mainly respiratory) requiring therapeutic intervention and in the total number of therapeutic interventions in calves fed high-fat CMR.
32113757	4	34	theme	CMR	641:643	arg1	kilogram					629:636	kilogram	629:636	kilogram of CMR	629:643	Lactose was exchanged for fat on a weight/weight basis, resulting in different energy contents per kilogram of CMR.
32113757	0	35	theme	ad	58:59	arg1	intake					74:79	ad libitum feed intake	58:79	ad libitum feed intake	58:79	Effects of exchanging lactose for fat in milk replacer on ad libitum feed intake and growth performance in dairy calves.
32113757	12	36	theme	components	1576:1585	arg1	intake					1554:1559	the intake	1550:1559	the intake of all dietary components	1550:1585	Measurements included the intake of all dietary components, body weight gain, and a selection of blood traits.
32113757	12	36	theme	components	1576:1585	arg1	gain					1600:1603	body weight gain	1588:1603	body weight gain	1588:1603	Measurements included the intake of all dietary components, body weight gain, and a selection of blood traits.
32113757	12	36	theme	components	1576:1585	arg1	selection					1612:1620	a selection	1610:1620	a selection of blood traits	1610:1636	Measurements included the intake of all dietary components, body weight gain, and a selection of blood traits.
32113757	14	37	dep	consumption	1853:1863	arg1	straw					1846:1850	straw	1846:1850	straw	1846:1850	Total solid feed (starter and straw) consumption was not affected by CMR composition.
32113757	14	37	dep	consumption	1853:1863	arg1	starter					1834:1840	starter	1834:1840	starter	1834:1840	Total solid feed (starter and straw) consumption was not affected by CMR composition.
32113757	0	38	from	Effects	0:6	arg1	intake					74:79	ad libitum feed intake	58:79	ad libitum feed intake	58:79	Effects of exchanging lactose for fat in milk replacer on ad libitum feed intake and growth performance in dairy calves.
32113757	0	38	from	Effects	0:6	arg1	replacer					46:53	milk replacer	41:53	milk replacer	41:53	Effects of exchanging lactose for fat in milk replacer on ad libitum feed intake and growth performance in dairy calves.
32113757	0	38	from	Effects	0:6	arg1	performance					92:102	growth performance	85:102	growth performance	85:102	Effects of exchanging lactose for fat in milk replacer on ad libitum feed intake and growth performance in dairy calves.
32113757	18	39	theme	body	2584:2587	arg1	gain					2596:2599	equal body weight gain	2578:2599	equal body weight gain	2578:2599	Calves appeared to consume CMR based on energy content, with a difference in ad libitum intake proportional to the difference in energy content of the CMR, maintaining equal body weight gain and solid feed intake.
32113757	0	40	theme	feed	69:72	arg1	intake					74:79	ad libitum feed intake	58:79	ad libitum feed intake	58:79	Effects of exchanging lactose for fat in milk replacer on ad libitum feed intake and growth performance in dairy calves.
32113757	13	41	theme	lactose	1680:1686	arg1	expense					1669:1675	the expense	1665:1675	the expense of lactose	1665:1686	Increasing fat content at the expense of lactose decreased CMR intake by 10%, whereas total calculated metabolizable energy intake and growth remained equal between treatments.
32113757	9	42	from	group-housed	1141:1152	arg1	period					1119:1124	period 2	1119:1126	period 2	1119:1126	In period 2, calves were group-housed with 8 calves per pen and received ad libitum access to their assigned CMR treatments, starter feed, chopped wheat straw, and water.
32113757	6	43	theme	treatments	836:845	arg1	1					829:829	1	829:829	1	829:829	Within each block, calves were randomly assigned to 1 of 2 treatments.
32113757	6	43	theme	treatments	836:845	arg1	treatments					836:845	2 treatments	834:845	2 treatments	834:845	Within each block, calves were randomly assigned to 1 of 2 treatments.
32113757	5	44	theme	0.12	676:679	arg1	±					674:674	±	674:674	±	674:674	Thirty-two male calves (1.7 ± 0.12 d of age, 47.6 ± 0.83 kg of body weight) were assigned to 1 of 16 blocks based on arrival date.
32113757	13	45	theme	CMR	1698:1700	arg1	intake					1702:1707	CMR intake	1698:1707	CMR intake	1698:1707	Increasing fat content at the expense of lactose decreased CMR intake by 10%, whereas total calculated metabolizable energy intake and growth remained equal between treatments.
32113757	10	46	theme	CMR	1365:1367	arg1	allowance					1369:1377	CMR allowance	1365:1377	CMR allowance	1365:1377	During period 3, from 43 until 63 d of age, calves were weaned by restricting CMR allowance in 2 steps, maintaining access to all other feeds.
32113757	5	47	theme	body	709:712	arg1	weight					714:719	body weight	709:719	body weight	709:719	Thirty-two male calves (1.7 ± 0.12 d of age, 47.6 ± 0.83 kg of body weight) were assigned to 1 of 16 blocks based on arrival date.
32113757	1	48	theme	replacer	238:245	arg1	formulations					253:264	calf milk replacer (CMR) formulations	228:264	calf milk replacer (CMR) formulations	228:264	The recent trend in the dairy industry toward ad libitum feeding of young calves merits reconsideration of calf milk replacer (CMR) formulations.
32113757	18	49	theme	energy	2539:2544	arg1	content					2546:2552	energy content	2539:2552	energy content of the CMR	2539:2563	Calves appeared to consume CMR based on energy content, with a difference in ad libitum intake proportional to the difference in energy content of the CMR, maintaining equal body weight gain and solid feed intake.
32113757	10	50	dep	other	1417:1421	arg1	feeds					1423:1427	feeds	1423:1427	feeds	1423:1427	During period 3, from 43 until 63 d of age, calves were weaned by restricting CMR allowance in 2 steps, maintaining access to all other feeds.
32113757	14	51	theme	CMR	1885:1887	arg1	composition					1889:1899	CMR composition	1885:1899	CMR composition	1885:1899	Total solid feed (starter and straw) consumption was not affected by CMR composition.
32113757	0	52	theme	exchanging	11:20	arg1	lactose					22:28	exchanging lactose	11:28	exchanging lactose for fat in milk replacer	11:53	Effects of exchanging lactose for fat in milk replacer on ad libitum feed intake and growth performance in dairy calves.
32113757	18	53	theme	feed	2611:2614	arg1	intake					2616:2621	solid feed intake	2605:2621	solid feed intake	2605:2621	Calves appeared to consume CMR based on energy content, with a difference in ad libitum intake proportional to the difference in energy content of the CMR, maintaining equal body weight gain and solid feed intake.
32113757	1	54	theme	formulations	253:264	arg1	reconsideration					209:223	reconsideration	209:223	reconsideration of calf milk replacer (CMR) formulations	209:264	The recent trend in the dairy industry toward ad libitum feeding of young calves merits reconsideration of calf milk replacer (CMR) formulations.
32113757	17	55	from	decrease	2227:2234	arg1	number					2249:2254	the total number	2239:2254	the total number of health events (mainly respiratory) requiring therapeutic intervention	2239:2327	Despite the limited animal numbers in the present experiment, there was a significant decrease in the total number of health events (mainly respiratory) requiring therapeutic intervention and in the total number of therapeutic interventions in calves fed high-fat CMR.
32113757	17	55	from	decrease	2227:2234	arg1	number					2346:2351	the total number	2336:2351	the total number of therapeutic interventions in calves fed high-fat CMR	2336:2407	Despite the limited animal numbers in the present experiment, there was a significant decrease in the total number of health events (mainly respiratory) requiring therapeutic intervention and in the total number of therapeutic interventions in calves fed high-fat CMR.
32113757	2	56	theme	intake	286:291	arg1	regulation					293:302	feed intake regulation	281:302	feed intake regulation in young calves provided with ad libitum milk and solid feeds	281:364	Additionally, feed intake regulation in young calves provided with ad libitum milk and solid feeds is insufficiently understood.
32113757	18	57	theme	equal	2578:2582	arg1	gain					2596:2599	equal body weight gain	2578:2599	equal body weight gain	2578:2599	Calves appeared to consume CMR based on energy content, with a difference in ad libitum intake proportional to the difference in energy content of the CMR, maintaining equal body weight gain and solid feed intake.
32113757	16	58	theme	nonesterified	2041:2053	arg1	acids					2061:2065	nonesterified fatty acids	2041:2065	nonesterified fatty acids	2041:2065	High-fat CMR increased plasma phosphate, nonesterified fatty acids, triglycerides, and bilirubin, whereas plasma glucose remained unchanged.
32113757	18	59	theme	weight	2589:2594	arg1	gain					2596:2599	equal body weight gain	2578:2599	equal body weight gain	2578:2599	Calves appeared to consume CMR based on energy content, with a difference in ad libitum intake proportional to the difference in energy content of the CMR, maintaining equal body weight gain and solid feed intake.
32113757	1	60	theme	calves	195:200	arg1	feeding					178:184	ad libitum feeding	167:184	ad libitum feeding of young calves	167:200	The recent trend in the dairy industry toward ad libitum feeding of young calves merits reconsideration of calf milk replacer (CMR) formulations.
32113757	5	61	theme	0.83	698:701	arg1	±					696:696	±	696:696	±	696:696	Thirty-two male calves (1.7 ± 0.12 d of age, 47.6 ± 0.83 kg of body weight) were assigned to 1 of 16 blocks based on arrival date.
32113757	0	62	from	performance	92:102	arg1	calves					113:118	dairy calves	107:118	dairy calves	107:118	Effects of exchanging lactose for fat in milk replacer on ad libitum feed intake and growth performance in dairy calves.
32113757	17	63	dep	events	2266:2271	arg1	respiratory					2281:2291	respiratory	2281:2291	respiratory	2281:2291	Despite the limited animal numbers in the present experiment, there was a significant decrease in the total number of health events (mainly respiratory) requiring therapeutic intervention and in the total number of therapeutic interventions in calves fed high-fat CMR.
32113757	5	64	theme	age	686:688	arg1	kg					703:704	47.6 ± 0.83 kg	691:704	47.6 ± 0.83 kg of body weight	691:719	Thirty-two male calves (1.7 ± 0.12 d of age, 47.6 ± 0.83 kg of body weight) were assigned to 1 of 16 blocks based on arrival date.
32113757	5	64	theme	age	686:688	arg1	d					681:681	1.7 ± 0.12 d	670:681	1.7 ± 0.12 d of age	670:688	Thirty-two male calves (1.7 ± 0.12 d of age, 47.6 ± 0.83 kg of body weight) were assigned to 1 of 16 blocks based on arrival date.
32113757	17	65	from	calves	2385:2390	arg1	number					2346:2351	the total number	2336:2351	the total number of therapeutic interventions in calves fed high-fat CMR	2336:2407	Despite the limited animal numbers in the present experiment, there was a significant decrease in the total number of health events (mainly respiratory) requiring therapeutic intervention and in the total number of therapeutic interventions in calves fed high-fat CMR.
32113757	3	66	theme	lactose	458:464	arg1	effect					437:442	the effect	433:442	the effect of exchanging lactose for fat in CMR on voluntary feed intake and growth performance	433:527	This study was designed to determine the effect of exchanging lactose for fat in CMR on voluntary feed intake and growth performance.
32113757	17	67	theme	animal	2161:2166	arg1	numbers					2168:2174	the limited animal numbers	2149:2174	the limited animal numbers in the present experiment	2149:2200	Despite the limited animal numbers in the present experiment, there was a significant decrease in the total number of health events (mainly respiratory) requiring therapeutic intervention and in the total number of therapeutic interventions in calves fed high-fat CMR.
32113757	3	68	theme	voluntary	484:492	arg1	intake					499:504	voluntary feed intake	484:504	voluntary feed intake	484:504	This study was designed to determine the effect of exchanging lactose for fat in CMR on voluntary feed intake and growth performance.
32113757	9	69	theme	libitum	1192:1198	arg1	access					1200:1205	ad libitum access	1189:1205	ad libitum access to their assigned CMR treatments, starter feed, chopped wheat straw, and water	1189:1284	In period 2, calves were group-housed with 8 calves per pen and received ad libitum access to their assigned CMR treatments, starter feed, chopped wheat straw, and water.
32113757	17	70	theme	total	2340:2344	arg1	number					2346:2351	the total number	2336:2351	the total number of therapeutic interventions in calves fed high-fat CMR	2336:2407	Despite the limited animal numbers in the present experiment, there was a significant decrease in the total number of health events (mainly respiratory) requiring therapeutic intervention and in the total number of therapeutic interventions in calves fed high-fat CMR.
32113757	17	71	from	numbers	2168:2174	arg1	experiment					2191:2200	the present experiment	2179:2200	the present experiment	2179:2200	Despite the limited animal numbers in the present experiment, there was a significant decrease in the total number of health events (mainly respiratory) requiring therapeutic intervention and in the total number of therapeutic interventions in calves fed high-fat CMR.
32113757	9	72	with	group-housed	1141:1152	arg1	calves					1161:1166	8 calves	1159:1166	8 calves per pen	1159:1174	In period 2, calves were group-housed with 8 calves per pen and received ad libitum access to their assigned CMR treatments, starter feed, chopped wheat straw, and water.
32113757	9	73	theme	assigned	1216:1223	arg1	treatments					1229:1238	their assigned CMR treatments	1210:1238	their assigned CMR treatments	1210:1238	In period 2, calves were group-housed with 8 calves per pen and received ad libitum access to their assigned CMR treatments, starter feed, chopped wheat straw, and water.
32113757	8	74	theme	assigned	992:999	arg1	treatments					1005:1014	their assigned CMR treatments	986:1014	their assigned CMR treatments	986:1014	In period 1, until 14 ± 1.7 d of age, calves were individually housed, restricted-fed their assigned CMR treatments at 2.5 to 3 L twice daily, and provided with unlimited access to water, chopped straw, and starter.
32113757	8	75	from	L	1028:1028	arg1	restricted-fed					971:984	restricted-fed	971:984	restricted-fed	971:984	In period 1, until 14 ± 1.7 d of age, calves were individually housed, restricted-fed their assigned CMR treatments at 2.5 to 3 L twice daily, and provided with unlimited access to water, chopped straw, and starter.
32113757	14	76	theme	solid	1822:1826	arg1	consumption					1853:1863	Total solid feed (starter and straw) consumption	1816:1863	Total solid feed (starter and straw) consumption	1816:1863	Total solid feed (starter and straw) consumption was not affected by CMR composition.
32113757	14	77	theme	feed	1828:1831	arg1	consumption					1853:1863	Total solid feed (starter and straw) consumption	1816:1863	Total solid feed (starter and straw) consumption	1816:1863	Total solid feed (starter and straw) consumption was not affected by CMR composition.
32113757	3	78	from	effect	437:442	arg1	CMR					477:479	CMR	477:479	CMR	477:479	This study was designed to determine the effect of exchanging lactose for fat in CMR on voluntary feed intake and growth performance.
32113757	3	78	from	effect	437:442	arg1	intake					499:504	voluntary feed intake	484:504	voluntary feed intake	484:504	This study was designed to determine the effect of exchanging lactose for fat in CMR on voluntary feed intake and growth performance.
32113757	3	78	from	effect	437:442	arg1	performance					517:527	growth performance	510:527	growth performance	510:527	This study was designed to determine the effect of exchanging lactose for fat in CMR on voluntary feed intake and growth performance.
32113757	3	79	theme	growth	510:515	arg1	performance					517:527	growth performance	510:527	growth performance	510:527	This study was designed to determine the effect of exchanging lactose for fat in CMR on voluntary feed intake and growth performance.
32113757	1	80	theme	dairy	145:149	arg1	industry					151:158	the dairy industry	141:158	the dairy industry	141:158	The recent trend in the dairy industry toward ad libitum feeding of young calves merits reconsideration of calf milk replacer (CMR) formulations.
32113757	17	81	theme	interventions	2368:2380	arg1	number					2346:2351	the total number	2336:2351	the total number of therapeutic interventions in calves fed high-fat CMR	2336:2407	Despite the limited animal numbers in the present experiment, there was a significant decrease in the total number of health events (mainly respiratory) requiring therapeutic intervention and in the total number of therapeutic interventions in calves fed high-fat CMR.
32113757	4	82	theme	energy	609:614	arg1	contents					616:623	different energy contents	599:623	different energy contents per kilogram of CMR	599:643	Lactose was exchanged for fat on a weight/weight basis, resulting in different energy contents per kilogram of CMR.
32113757	14	83	theme	Total	1816:1820	arg1	consumption					1853:1863	Total solid feed (starter and straw) consumption	1816:1863	Total solid feed (starter and straw) consumption	1816:1863	Total solid feed (starter and straw) consumption was not affected by CMR composition.
32113757	16	84	theme	High-fat	2000:2007	arg1	CMR					2009:2011	High-fat CMR	2000:2011	High-fat CMR	2000:2011	High-fat CMR increased plasma phosphate, nonesterified fatty acids, triglycerides, and bilirubin, whereas plasma glucose remained unchanged.
32113757	12	85	theme	blood	1625:1629	arg1	traits					1631:1636	blood traits	1625:1636	blood traits	1625:1636	Measurements included the intake of all dietary components, body weight gain, and a selection of blood traits.
32113757	17	86	from	number	2346:2351	arg1	calves					2385:2390	calves	2385:2390	calves fed high-fat CMR	2385:2407	Despite the limited animal numbers in the present experiment, there was a significant decrease in the total number of health events (mainly respiratory) requiring therapeutic intervention and in the total number of therapeutic interventions in calves fed high-fat CMR.
32113757	4	87	theme	weight/weight	565:577	arg1	basis					579:583	a weight/weight basis	563:583	a weight/weight basis	563:583	Lactose was exchanged for fat on a weight/weight basis, resulting in different energy contents per kilogram of CMR.
32113757	5	88	theme	male	657:660	arg1	calves					662:667	Thirty-two male calves	646:667	Thirty-two male calves (1.7 ± 0.12 d of age, 47.6 ± 0.83 kg of body weight)	646:720	Thirty-two male calves (1.7 ± 0.12 d of age, 47.6 ± 0.83 kg of body weight) were assigned to 1 of 16 blocks based on arrival date.
32113757	9	89	theme	starter	1241:1247	arg1	feed					1249:1252	starter feed	1241:1252	starter feed	1241:1252	In period 2, calves were group-housed with 8 calves per pen and received ad libitum access to their assigned CMR treatments, starter feed, chopped wheat straw, and water.
32113757	17	90	theme	significant	2215:2225	arg1	decrease					2227:2234	a significant decrease	2213:2234	a significant decrease in the total number of health events (mainly respiratory) requiring therapeutic intervention and in the total number of therapeutic interventions in calves fed high-fat CMR	2213:2407	Despite the limited animal numbers in the present experiment, there was a significant decrease in the total number of health events (mainly respiratory) requiring therapeutic intervention and in the total number of therapeutic interventions in calves fed high-fat CMR.
32113757	11	91	theme	age	1512:1514	arg1	d					1507:1507	77 d	1504:1507	77 d of age (period 4)	1504:1525	All calves were completely weaned at d 64 of age and were monitored until 77 d of age (period 4).
32113757	11	91	theme	age	1512:1514	arg1	period					1517:1522	period 4	1517:1524	period 4	1517:1524	All calves were completely weaned at d 64 of age and were monitored until 77 d of age (period 4).
32113757	13	92	theme	total	1725:1729	arg1	intake					1763:1768	total calculated metabolizable energy intake	1725:1768	total calculated metabolizable energy intake	1725:1768	Increasing fat content at the expense of lactose decreased CMR intake by 10%, whereas total calculated metabolizable energy intake and growth remained equal between treatments.
32113757	9	93	theme	wheat	1263:1267	arg1	straw					1269:1273	chopped wheat straw	1255:1273	chopped wheat straw	1255:1273	In period 2, calves were group-housed with 8 calves per pen and received ad libitum access to their assigned CMR treatments, starter feed, chopped wheat straw, and water.
32113757	0	94	from	intake	74:79	arg1	calves					113:118	dairy calves	107:118	dairy calves	107:118	Effects of exchanging lactose for fat in milk replacer on ad libitum feed intake and growth performance in dairy calves.
32113757	17	95	theme	total	2243:2247	arg1	number					2249:2254	the total number	2239:2254	the total number of health events (mainly respiratory) requiring therapeutic intervention	2239:2327	Despite the limited animal numbers in the present experiment, there was a significant decrease in the total number of health events (mainly respiratory) requiring therapeutic intervention and in the total number of therapeutic interventions in calves fed high-fat CMR.
32113757	13	96	theme	metabolizable	1742:1754	arg1	intake					1763:1768	total calculated metabolizable energy intake	1725:1768	total calculated metabolizable energy intake	1725:1768	Increasing fat content at the expense of lactose decreased CMR intake by 10%, whereas total calculated metabolizable energy intake and growth remained equal between treatments.
32113757	7	97	theme	experimental	852:863	arg1	period					865:870	The experimental period	848:870	The experimental period	848:870	The experimental period was divided into 4 periods.
32113757	5	98	dep	calves	662:667	arg1	kg					703:704	47.6 ± 0.83 kg	691:704	47.6 ± 0.83 kg of body weight	691:719	Thirty-two male calves (1.7 ± 0.12 d of age, 47.6 ± 0.83 kg of body weight) were assigned to 1 of 16 blocks based on arrival date.
32113757	5	98	dep	calves	662:667	arg1	d					681:681	1.7 ± 0.12 d	670:681	1.7 ± 0.12 d of age	670:688	Thirty-two male calves (1.7 ± 0.12 d of age, 47.6 ± 0.83 kg of body weight) were assigned to 1 of 16 blocks based on arrival date.
32113757	0	99	from	lactose	22:28	arg1	replacer					46:53	milk replacer	41:53	milk replacer	41:53	Effects of exchanging lactose for fat in milk replacer on ad libitum feed intake and growth performance in dairy calves.
32113757	15	100	theme	ad	1938:1939	arg1	libitum					1941:1947	ad libitum	1938:1947	ad libitum	1938:1947	These data indicate that calves fed ad libitum regulate their CMR intake based on energy content.
32113757	3	101	from	CMR	477:479	arg1	effect					437:442	the effect	433:442	the effect of exchanging lactose for fat in CMR on voluntary feed intake and growth performance	433:527	This study was designed to determine the effect of exchanging lactose for fat in CMR on voluntary feed intake and growth performance.
32113757	5	102	theme	1.7	670:672	arg1	±					674:674	±	674:674	±	674:674	Thirty-two male calves (1.7 ± 0.12 d of age, 47.6 ± 0.83 kg of body weight) were assigned to 1 of 16 blocks based on arrival date.
32113757	0	103	theme	libitum	61:67	arg1	intake					74:79	ad libitum feed intake	58:79	ad libitum feed intake	58:79	Effects of exchanging lactose for fat in milk replacer on ad libitum feed intake and growth performance in dairy calves.
32113757	16	104	dep	increased	2013:2021	arg1	whereas					2098:2104	whereas	2098:2104	whereas	2098:2104	High-fat CMR increased plasma phosphate, nonesterified fatty acids, triglycerides, and bilirubin, whereas plasma glucose remained unchanged.
32113757	1	105	theme	recent	125:130	arg1	trend					132:136	The recent trend	121:136	The recent trend in the dairy industry toward ad libitum feeding of young calves	121:200	The recent trend in the dairy industry toward ad libitum feeding of young calves merits reconsideration of calf milk replacer (CMR) formulations.
32113757	18	106	theme	proportional	2505:2516	arg1	intake					2498:2503	ad libitum intake	2487:2503	ad libitum intake proportional to the difference in energy content of the CMR	2487:2563	Calves appeared to consume CMR based on energy content, with a difference in ad libitum intake proportional to the difference in energy content of the CMR, maintaining equal body weight gain and solid feed intake.
32113757	12	107	theme	body	1588:1591	arg1	gain					1600:1603	body weight gain	1588:1603	body weight gain	1588:1603	Measurements included the intake of all dietary components, body weight gain, and a selection of blood traits.
32113757	11	108	theme	age	1475:1477	arg1	d					1467:1467	d 64	1467:1470	d 64 of age	1467:1477	All calves were completely weaned at d 64 of age and were monitored until 77 d of age (period 4).
32113757	12	109	theme	weight	1593:1598	arg1	gain					1600:1603	body weight gain	1588:1603	body weight gain	1588:1603	Measurements included the intake of all dietary components, body weight gain, and a selection of blood traits.
32113757	1	110	from	trend	132:136	arg1	industry					151:158	the dairy industry	141:158	the dairy industry	141:158	The recent trend in the dairy industry toward ad libitum feeding of young calves merits reconsideration of calf milk replacer (CMR) formulations.
32113757	17	111	theme	present	2183:2189	arg1	experiment					2191:2200	the present experiment	2179:2200	the present experiment	2179:2200	Despite the limited animal numbers in the present experiment, there was a significant decrease in the total number of health events (mainly respiratory) requiring therapeutic intervention and in the total number of therapeutic interventions in calves fed high-fat CMR.
32113757	0	112	theme	growth	85:90	arg1	performance					92:102	growth performance	85:102	growth performance	85:102	Effects of exchanging lactose for fat in milk replacer on ad libitum feed intake and growth performance in dairy calves.
32113757	8	113	theme	unlimited	1061:1069	arg1	access					1071:1076	unlimited access	1061:1076	unlimited access to water, chopped straw, and starter	1061:1113	In period 1, until 14 ± 1.7 d of age, calves were individually housed, restricted-fed their assigned CMR treatments at 2.5 to 3 L twice daily, and provided with unlimited access to water, chopped straw, and starter.
32113757	15	114	theme	CMR	1964:1966	arg1	intake					1968:1973	their CMR intake	1958:1973	their CMR intake	1958:1973	These data indicate that calves fed ad libitum regulate their CMR intake based on energy content.
32113757	18	115	theme	ad	2487:2488	arg1	intake					2498:2503	ad libitum intake	2487:2503	ad libitum intake proportional to the difference in energy content of the CMR	2487:2563	Calves appeared to consume CMR based on energy content, with a difference in ad libitum intake proportional to the difference in energy content of the CMR, maintaining equal body weight gain and solid feed intake.
32113757	9	116	from	period	1119:1124	arg1	group-housed					1141:1152	group-housed	1141:1152	group-housed	1141:1152	In period 2, calves were group-housed with 8 calves per pen and received ad libitum access to their assigned CMR treatments, starter feed, chopped wheat straw, and water.
32113757	2	117	theme	ad	334:335	arg1	libitum					337:343	ad libitum	334:343	ad libitum milk	334:348	Additionally, feed intake regulation in young calves provided with ad libitum milk and solid feeds is insufficiently understood.
32113757	0	118	theme	lactose	22:28	arg1	Effects					0:6	Effects	0:6	Effects of exchanging lactose for fat in milk replacer on ad libitum feed intake and growth performance in dairy calves.	0:119	Effects of exchanging lactose for fat in milk replacer on ad libitum feed intake and growth performance in dairy calves.
32113757	8	119	theme	±	922:922	arg1	d					928:928	14 ± 1.7 d	919:928	14 ± 1.7 d of age	919:935	In period 1, until 14 ± 1.7 d of age, calves were individually housed, restricted-fed their assigned CMR treatments at 2.5 to 3 L twice daily, and provided with unlimited access to water, chopped straw, and starter.
32113757	17	120	theme	high-fat	2396:2403	arg1	CMR					2405:2407	high-fat CMR	2396:2407	high-fat CMR	2396:2407	Despite the limited animal numbers in the present experiment, there was a significant decrease in the total number of health events (mainly respiratory) requiring therapeutic intervention and in the total number of therapeutic interventions in calves fed high-fat CMR.
32113757	5	121	theme	47.6	691:694	arg1	±					696:696	±	696:696	±	696:696	Thirty-two male calves (1.7 ± 0.12 d of age, 47.6 ± 0.83 kg of body weight) were assigned to 1 of 16 blocks based on arrival date.
32113757	18	122	theme	solid	2605:2609	arg1	intake					2616:2621	solid feed intake	2605:2621	solid feed intake	2605:2621	Calves appeared to consume CMR based on energy content, with a difference in ad libitum intake proportional to the difference in energy content of the CMR, maintaining equal body weight gain and solid feed intake.
32113757	1	123	theme	libitum	170:176	arg1	feeding					178:184	ad libitum feeding	167:184	ad libitum feeding of young calves	167:200	The recent trend in the dairy industry toward ad libitum feeding of young calves merits reconsideration of calf milk replacer (CMR) formulations.
32113757	8	124	dep	3	1026:1026	arg1	to					1023:1024	to	1023:1024	to	1023:1024	In period 1, until 14 ± 1.7 d of age, calves were individually housed, restricted-fed their assigned CMR treatments at 2.5 to 3 L twice daily, and provided with unlimited access to water, chopped straw, and starter.
32113757	2	125	dep	solid	354:358	arg1	feeds					360:364	feeds	360:364	feeds	360:364	Additionally, feed intake regulation in young calves provided with ad libitum milk and solid feeds is insufficiently understood.
32113757	16	126	theme	plasma	2023:2028	arg1	phosphate					2030:2038	plasma phosphate	2023:2038	plasma phosphate	2023:2038	High-fat CMR increased plasma phosphate, nonesterified fatty acids, triglycerides, and bilirubin, whereas plasma glucose remained unchanged.
32113757	0	127	theme	milk	41:44	arg1	replacer					46:53	milk replacer	41:53	milk replacer	41:53	Effects of exchanging lactose for fat in milk replacer on ad libitum feed intake and growth performance in dairy calves.
32113757	8	128	theme	age	933:935	arg1	d					928:928	14 ± 1.7 d	919:928	14 ± 1.7 d of age	919:935	In period 1, until 14 ± 1.7 d of age, calves were individually housed, restricted-fed their assigned CMR treatments at 2.5 to 3 L twice daily, and provided with unlimited access to water, chopped straw, and starter.
32113757	18	129	theme	libitum	2490:2496	arg1	intake					2498:2503	ad libitum intake	2487:2503	ad libitum intake proportional to the difference in energy content of the CMR	2487:2563	Calves appeared to consume CMR based on energy content, with a difference in ad libitum intake proportional to the difference in energy content of the CMR, maintaining equal body weight gain and solid feed intake.
32113757	3	130	theme	exchanging	447:456	arg1	lactose					458:464	exchanging lactose	447:464	exchanging lactose for fat in CMR	447:479	This study was designed to determine the effect of exchanging lactose for fat in CMR on voluntary feed intake and growth performance.
32113757	18	131	theme	energy	2450:2455	arg1	content					2457:2463	energy content	2450:2463	energy content	2450:2463	Calves appeared to consume CMR based on energy content, with a difference in ad libitum intake proportional to the difference in energy content of the CMR, maintaining equal body weight gain and solid feed intake.
32113757	16	132	theme	fatty	2055:2059	arg1	acids					2061:2065	nonesterified fatty acids	2041:2065	nonesterified fatty acids	2041:2065	High-fat CMR increased plasma phosphate, nonesterified fatty acids, triglycerides, and bilirubin, whereas plasma glucose remained unchanged.
32113757	2	133	theme	feed	281:284	arg1	regulation					293:302	feed intake regulation	281:302	feed intake regulation in young calves provided with ad libitum milk and solid feeds	281:364	Additionally, feed intake regulation in young calves provided with ad libitum milk and solid feeds is insufficiently understood.
32113757	1	134	theme	young	189:193	arg1	calves					195:200	young calves	189:200	young calves	189:200	The recent trend in the dairy industry toward ad libitum feeding of young calves merits reconsideration of calf milk replacer (CMR) formulations.
32113757	13	135	theme	fat	1650:1652	arg1	content					1654:1660	fat content	1650:1660	fat content	1650:1660	Increasing fat content at the expense of lactose decreased CMR intake by 10%, whereas total calculated metabolizable energy intake and growth remained equal between treatments.
32113757	18	136	theme	CMR	2561:2563	arg1	content					2546:2552	energy content	2539:2552	energy content of the CMR	2539:2563	Calves appeared to consume CMR based on energy content, with a difference in ad libitum intake proportional to the difference in energy content of the CMR, maintaining equal body weight gain and solid feed intake.
32751784	7	0	theme	metabolome	1205:1214	arg1	Analysis					1170:1177	Analysis	1170:1177	Analysis of 16S rDNA sequences and metabolome	1170:1214	Analysis of 16S rDNA sequences and metabolome demonstrates that L. reuteri I5007 significantly alters colonic microbiota and metabolic structural and functional composition.
32751784	6	1	theme	length	1041:1046	arg1	shortening					1048:1057	colon length shortening	1035:1057	colon length shortening	1035:1057	L. reuteri I5007 improved DSS-induced colitis, which was confirmed by reduced weight loss, colon length shortening, and histopathological damage, restored the mucus layer, as well as reduced pro-inflammatory cytokines levels.
32751784	3	2	dep	co-	598:600	arg1	simultaneous					603:614	simultaneous	603:614	simultaneous	603:614	In vitro, HT-29 cells were cultured with L. reuteri I5007 or lipopolysaccharide treatment under three different conditions, i.e., pre-, co- (simultaneous), and posttreatment.
32751784	5	3	theme	L.	782:783	arg1	I5007					793:797	L. reuteri I5007	782:797	L. reuteri I5007 by gavage	782:807	In vivo, mice were given L. reuteri I5007 by gavage throughout the study, starting one week prior to dextran sulfate sodium (DSS) treatment for one week followed by two days without DSS.
32751784	1	4	theme	immunity	274:281	arg1	regulation					260:269	the regulation	256:269	the regulation of immunity and intestinal microbiota	256:307	Lactobacillus reuteri, a commensal intestinal bacteria, has various health benefits including the regulation of immunity and intestinal microbiota.
32751784	0	5	theme	Metabolic	91:99	arg1	Disorders					101:109	Metabolic Disorders	91:109	Metabolic Disorders	91:109	Lactobacillus reuteri Ameliorates Intestinal Inflammation and Modulates Gut Microbiota and Metabolic Disorders in Dextran Sulfate Sodium-Induced Colitis in Mice.
32751784	8	6	theme	structure	1542:1550	arg1	composition					1512:1522	the composition	1508:1522	the composition of gut microbiota structure and function	1508:1563	Overall, the results demonstrate that L. reuteri I5007 pretreatment could effectively alleviate intestinal inflammation by regulating immune responses and altering the composition of gut microbiota structure and function, as well as improving metabolic disorders in mice with colitis.
32751784	6	7	dep	L.	944:945	arg1	reuteri					947:953	reuteri	947:953	reuteri	947:953	L. reuteri I5007 improved DSS-induced colitis, which was confirmed by reduced weight loss, colon length shortening, and histopathological damage, restored the mucus layer, as well as reduced pro-inflammatory cytokines levels.
32751784	0	8	theme	Dextran	114:120	arg1	Sulfate					122:128	Dextran Sulfate	114:128	Dextran Sulfate Sodium-Induced Colitis in Mice	114:159	Lactobacillus reuteri Ameliorates Intestinal Inflammation and Modulates Gut Microbiota and Metabolic Disorders in Dextran Sulfate Sodium-Induced Colitis in Mice.
32751784	6	9	theme	colon	1035:1039	arg1	shortening					1048:1057	colon length shortening	1035:1057	colon length shortening	1035:1057	L. reuteri I5007 improved DSS-induced colitis, which was confirmed by reduced weight loss, colon length shortening, and histopathological damage, restored the mucus layer, as well as reduced pro-inflammatory cytokines levels.
32751784	1	10	theme	intestinal	287:296	arg1	microbiota					298:307	intestinal microbiota	287:307	intestinal microbiota	287:307	Lactobacillus reuteri, a commensal intestinal bacteria, has various health benefits including the regulation of immunity and intestinal microbiota.
32751784	7	11	theme	rDNA	1186:1189	arg1	sequences					1191:1199	16S rDNA sequences	1182:1199	16S rDNA sequences	1182:1199	Analysis of 16S rDNA sequences and metabolome demonstrates that L. reuteri I5007 significantly alters colonic microbiota and metabolic structural and functional composition.
32751784	4	12	theme	L.	655:656	arg1	I5007					666:670	L. reuteri I5007	655:670	L. reuteri I5007	655:670	Pretreatment with L. reuteri I5007 effectively relieves inflammation in HT-29 cells challenged with lipopolysaccharide.
32751784	8	13	from	disorders	1597:1605	arg1	mice					1610:1613	mice	1610:1613	mice with colitis	1610:1626	Overall, the results demonstrate that L. reuteri I5007 pretreatment could effectively alleviate intestinal inflammation by regulating immune responses and altering the composition of gut microbiota structure and function, as well as improving metabolic disorders in mice with colitis.
32751784	1	14	theme	microbiota	298:307	arg1	regulation					260:269	the regulation	256:269	the regulation of immunity and intestinal microbiota	256:307	Lactobacillus reuteri, a commensal intestinal bacteria, has various health benefits including the regulation of immunity and intestinal microbiota.
32751784	0	15	theme	Sodium-Induced	130:143	arg1	Colitis					145:151	Dextran Sulfate Sodium-Induced Colitis	114:151	Dextran Sulfate Sodium-Induced Colitis in Mice	114:159	Lactobacillus reuteri Ameliorates Intestinal Inflammation and Modulates Gut Microbiota and Metabolic Disorders in Dextran Sulfate Sodium-Induced Colitis in Mice.
32751784	5	16	theme	dextran	858:864	arg1	DSS					882:884	DSS	882:884	DSS	882:884	In vivo, mice were given L. reuteri I5007 by gavage throughout the study, starting one week prior to dextran sulfate sodium (DSS) treatment for one week followed by two days without DSS.
32751784	5	16	theme	dextran	858:864	arg1	sodium					874:879	dextran sulfate sodium	858:879	dextran sulfate sodium (DSS) treatment for one week followed by two days without DSS	858:941	In vivo, mice were given L. reuteri I5007 by gavage throughout the study, starting one week prior to dextran sulfate sodium (DSS) treatment for one week followed by two days without DSS.
32751784	3	17	theme	L.	503:504	arg1	I5007					514:518	L. reuteri I5007	503:518	L. reuteri I5007	503:518	In vitro, HT-29 cells were cultured with L. reuteri I5007 or lipopolysaccharide treatment under three different conditions, i.e., pre-, co- (simultaneous), and posttreatment.
32751784	8	18	theme	L.	1382:1383	arg1	pretreatment					1399:1410	L. reuteri I5007 pretreatment	1382:1410	L. reuteri I5007 pretreatment	1382:1410	Overall, the results demonstrate that L. reuteri I5007 pretreatment could effectively alleviate intestinal inflammation by regulating immune responses and altering the composition of gut microbiota structure and function, as well as improving metabolic disorders in mice with colitis.
32751784	1	19	theme	commensal	187:195	arg1	bacteria					208:215	a commensal intestinal bacteria	185:215	a commensal intestinal bacteria	185:215	Lactobacillus reuteri, a commensal intestinal bacteria, has various health benefits including the regulation of immunity and intestinal microbiota.
32751784	0	20	theme	Sulfate	122:128	arg1	Colitis					145:151	Dextran Sulfate Sodium-Induced Colitis	114:151	Dextran Sulfate Sodium-Induced Colitis in Mice	114:159	Lactobacillus reuteri Ameliorates Intestinal Inflammation and Modulates Gut Microbiota and Metabolic Disorders in Dextran Sulfate Sodium-Induced Colitis in Mice.
32751784	8	21	theme	metabolic	1587:1595	arg1	disorders					1597:1605	metabolic disorders	1587:1605	metabolic disorders in mice with colitis	1587:1626	Overall, the results demonstrate that L. reuteri I5007 pretreatment could effectively alleviate intestinal inflammation by regulating immune responses and altering the composition of gut microbiota structure and function, as well as improving metabolic disorders in mice with colitis.
32751784	1	22	theme	intestinal	197:206	arg1	bacteria					208:215	a commensal intestinal bacteria	185:215	a commensal intestinal bacteria	185:215	Lactobacillus reuteri, a commensal intestinal bacteria, has various health benefits including the regulation of immunity and intestinal microbiota.
32751784	7	23	theme	structural	1305:1314	arg1	composition					1331:1341	metabolic structural and functional composition	1295:1341	metabolic structural and functional composition	1295:1341	Analysis of 16S rDNA sequences and metabolome demonstrates that L. reuteri I5007 significantly alters colonic microbiota and metabolic structural and functional composition.
32751784	6	24	theme	cytokines	1152:1160	arg1	levels					1162:1167	pro-inflammatory cytokines levels	1135:1167	pro-inflammatory cytokines levels	1135:1167	L. reuteri I5007 improved DSS-induced colitis, which was confirmed by reduced weight loss, colon length shortening, and histopathological damage, restored the mucus layer, as well as reduced pro-inflammatory cytokines levels.
32751784	4	25	theme	HT-29	709:713	arg1	cells					715:719	HT-29 cells	709:719	HT-29 cells challenged with lipopolysaccharide	709:754	Pretreatment with L. reuteri I5007 effectively relieves inflammation in HT-29 cells challenged with lipopolysaccharide.
32751784	8	26	theme	microbiota	1531:1540	arg1	structure					1542:1550	gut microbiota structure	1527:1550	gut microbiota structure	1527:1550	Overall, the results demonstrate that L. reuteri I5007 pretreatment could effectively alleviate intestinal inflammation by regulating immune responses and altering the composition of gut microbiota structure and function, as well as improving metabolic disorders in mice with colitis.
32751784	6	27	theme	pro-inflammatory	1135:1150	arg1	levels					1162:1167	pro-inflammatory cytokines levels	1135:1167	pro-inflammatory cytokines levels	1135:1167	L. reuteri I5007 improved DSS-induced colitis, which was confirmed by reduced weight loss, colon length shortening, and histopathological damage, restored the mucus layer, as well as reduced pro-inflammatory cytokines levels.
32751784	2	28	theme	modulating	412:421	arg1	microbiota					423:432	modulating microbiota	412:432	modulating microbiota	412:432	We examined whether L. reuteri I5007 could protect mice against colitis in ameliorating inflammation, modulating microbiota, and metabolic composition.
32751784	0	29	theme	Intestinal	34:43	arg1	Inflammation					45:56	Intestinal Inflammation	34:56	Intestinal Inflammation	34:56	Lactobacillus reuteri Ameliorates Intestinal Inflammation and Modulates Gut Microbiota and Metabolic Disorders in Dextran Sulfate Sodium-Induced Colitis in Mice.
32751784	6	30	theme	DSS-induced	970:980	arg1	colitis					982:988	DSS-induced colitis	970:988	DSS-induced colitis	970:988	L. reuteri I5007 improved DSS-induced colitis, which was confirmed by reduced weight loss, colon length shortening, and histopathological damage, restored the mucus layer, as well as reduced pro-inflammatory cytokines levels.
32751784	8	31	theme	intestinal	1440:1449	arg1	inflammation					1451:1462	intestinal inflammation	1440:1462	intestinal inflammation	1440:1462	Overall, the results demonstrate that L. reuteri I5007 pretreatment could effectively alleviate intestinal inflammation by regulating immune responses and altering the composition of gut microbiota structure and function, as well as improving metabolic disorders in mice with colitis.
32751784	1	32	dep	Lactobacillus	162:174	arg1	bacteria					208:215	a commensal intestinal bacteria	185:215	a commensal intestinal bacteria	185:215	Lactobacillus reuteri, a commensal intestinal bacteria, has various health benefits including the regulation of immunity and intestinal microbiota.
32751784	1	32	dep	Lactobacillus	162:174	arg1	reuteri					176:182	Lactobacillus reuteri, a commensal intestinal bacteria,	162:216	reuteri	176:182	Lactobacillus reuteri, a commensal intestinal bacteria, has various health benefits including the regulation of immunity and intestinal microbiota.
32751784	8	33	theme	function	1556:1563	arg1	composition					1512:1522	the composition	1508:1522	the composition of gut microbiota structure and function	1508:1563	Overall, the results demonstrate that L. reuteri I5007 pretreatment could effectively alleviate intestinal inflammation by regulating immune responses and altering the composition of gut microbiota structure and function, as well as improving metabolic disorders in mice with colitis.
32751784	7	34	theme	functional	1320:1329	arg1	composition					1331:1341	metabolic structural and functional composition	1295:1341	metabolic structural and functional composition	1295:1341	Analysis of 16S rDNA sequences and metabolome demonstrates that L. reuteri I5007 significantly alters colonic microbiota and metabolic structural and functional composition.
32751784	5	35	theme	prior	849:853	arg1	week					844:847	one week	840:847	one week prior to dextran sulfate sodium (DSS) treatment for one week followed by two days without DSS	840:941	In vivo, mice were given L. reuteri I5007 by gavage throughout the study, starting one week prior to dextran sulfate sodium (DSS) treatment for one week followed by two days without DSS.
32751784	0	36	from	Disorders	101:109	arg1	Colitis					145:151	Dextran Sulfate Sodium-Induced Colitis	114:151	Dextran Sulfate Sodium-Induced Colitis in Mice	114:159	Lactobacillus reuteri Ameliorates Intestinal Inflammation and Modulates Gut Microbiota and Metabolic Disorders in Dextran Sulfate Sodium-Induced Colitis in Mice.
32751784	2	37	theme	L.	330:331	arg1	I5007					341:345	L. reuteri I5007	330:345	L. reuteri I5007	330:345	We examined whether L. reuteri I5007 could protect mice against colitis in ameliorating inflammation, modulating microbiota, and metabolic composition.
32751784	1	38	theme	various	222:228	arg1	regulation					260:269	the regulation	256:269	the regulation of immunity and intestinal microbiota	256:307	Lactobacillus reuteri, a commensal intestinal bacteria, has various health benefits including the regulation of immunity and intestinal microbiota.
32751784	1	38	theme	various	222:228	arg1	benefits					237:244	various health benefits	222:244	various health benefits including the regulation of immunity and intestinal microbiota	222:307	Lactobacillus reuteri, a commensal intestinal bacteria, has various health benefits including the regulation of immunity and intestinal microbiota.
32751784	7	39	theme	sequences	1191:1199	arg1	Analysis					1170:1177	Analysis	1170:1177	Analysis of 16S rDNA sequences and metabolome	1170:1214	Analysis of 16S rDNA sequences and metabolome demonstrates that L. reuteri I5007 significantly alters colonic microbiota and metabolic structural and functional composition.
32751784	3	40	theme	lipopolysaccharide	523:540	arg1	treatment					542:550	lipopolysaccharide treatment	523:550	lipopolysaccharide treatment	523:550	In vitro, HT-29 cells were cultured with L. reuteri I5007 or lipopolysaccharide treatment under three different conditions, i.e., pre-, co- (simultaneous), and posttreatment.
32751784	5	41	theme	sulfate	866:872	arg1	DSS					882:884	DSS	882:884	DSS	882:884	In vivo, mice were given L. reuteri I5007 by gavage throughout the study, starting one week prior to dextran sulfate sodium (DSS) treatment for one week followed by two days without DSS.
32751784	5	41	theme	sulfate	866:872	arg1	sodium					874:879	dextran sulfate sodium	858:879	dextran sulfate sodium (DSS) treatment for one week followed by two days without DSS	858:941	In vivo, mice were given L. reuteri I5007 by gavage throughout the study, starting one week prior to dextran sulfate sodium (DSS) treatment for one week followed by two days without DSS.
32751784	1	42	theme	health	230:235	arg1	regulation					260:269	the regulation	256:269	the regulation of immunity and intestinal microbiota	256:307	Lactobacillus reuteri, a commensal intestinal bacteria, has various health benefits including the regulation of immunity and intestinal microbiota.
32751784	1	42	theme	health	230:235	arg1	benefits					237:244	various health benefits	222:244	various health benefits including the regulation of immunity and intestinal microbiota	222:307	Lactobacillus reuteri, a commensal intestinal bacteria, has various health benefits including the regulation of immunity and intestinal microbiota.
32751784	3	43	theme	HT-29	472:476	arg1	cells					478:482	HT-29 cells	472:482	HT-29 cells	472:482	In vitro, HT-29 cells were cultured with L. reuteri I5007 or lipopolysaccharide treatment under three different conditions, i.e., pre-, co- (simultaneous), and posttreatment.
32751784	7	44	dep	L.	1234:1235	arg1	reuteri					1237:1243	reuteri	1237:1243	reuteri	1237:1243	Analysis of 16S rDNA sequences and metabolome demonstrates that L. reuteri I5007 significantly alters colonic microbiota and metabolic structural and functional composition.
32751784	4	45	with	Pretreatment	637:648	arg1	I5007					666:670	L. reuteri I5007	655:670	L. reuteri I5007	655:670	Pretreatment with L. reuteri I5007 effectively relieves inflammation in HT-29 cells challenged with lipopolysaccharide.
32751784	5	46	theme	sodium	874:879	arg1	treatment					887:895	dextran sulfate sodium (DSS) treatment	858:895	dextran sulfate sodium (DSS) treatment for one week followed by two days without DSS	858:941	In vivo, mice were given L. reuteri I5007 by gavage throughout the study, starting one week prior to dextran sulfate sodium (DSS) treatment for one week followed by two days without DSS.
32751784	2	47	theme	metabolic	439:447	arg1	composition					449:459	metabolic composition	439:459	metabolic composition	439:459	We examined whether L. reuteri I5007 could protect mice against colitis in ameliorating inflammation, modulating microbiota, and metabolic composition.
32751784	0	48	theme	Gut	72:74	arg1	Microbiota					76:85	Gut Microbiota	72:85	Gut Microbiota	72:85	Lactobacillus reuteri Ameliorates Intestinal Inflammation and Modulates Gut Microbiota and Metabolic Disorders in Dextran Sulfate Sodium-Induced Colitis in Mice.
32751784	6	49	theme	L.	944:945	arg1	I5007					955:959	L. reuteri I5007	944:959	L. reuteri I5007	944:959	L. reuteri I5007 improved DSS-induced colitis, which was confirmed by reduced weight loss, colon length shortening, and histopathological damage, restored the mucus layer, as well as reduced pro-inflammatory cytokines levels.
32751784	8	50	theme	I5007	1393:1397	arg1	pretreatment					1399:1410	L. reuteri I5007 pretreatment	1382:1410	L. reuteri I5007 pretreatment	1382:1410	Overall, the results demonstrate that L. reuteri I5007 pretreatment could effectively alleviate intestinal inflammation by regulating immune responses and altering the composition of gut microbiota structure and function, as well as improving metabolic disorders in mice with colitis.
32751784	0	51	from	Microbiota	76:85	arg1	Colitis					145:151	Dextran Sulfate Sodium-Induced Colitis	114:151	Dextran Sulfate Sodium-Induced Colitis in Mice	114:159	Lactobacillus reuteri Ameliorates Intestinal Inflammation and Modulates Gut Microbiota and Metabolic Disorders in Dextran Sulfate Sodium-Induced Colitis in Mice.
32751784	3	52	dep	conditions	574:583	arg1	i.e.					586:589	three different conditions, i.e., pre-, co- (simultaneous), and posttreatment	558:634	i.e.	586:589	In vitro, HT-29 cells were cultured with L. reuteri I5007 or lipopolysaccharide treatment under three different conditions, i.e., pre-, co- (simultaneous), and posttreatment.
32751784	5	53	dep	L.	782:783	arg1	reuteri					785:791	reuteri	785:791	reuteri	785:791	In vivo, mice were given L. reuteri I5007 by gavage throughout the study, starting one week prior to dextran sulfate sodium (DSS) treatment for one week followed by two days without DSS.
32751784	8	54	theme	gut	1527:1529	arg1	structure					1542:1550	gut microbiota structure	1527:1550	gut microbiota structure	1527:1550	Overall, the results demonstrate that L. reuteri I5007 pretreatment could effectively alleviate intestinal inflammation by regulating immune responses and altering the composition of gut microbiota structure and function, as well as improving metabolic disorders in mice with colitis.
32751784	4	55	dep	L.	655:656	arg1	reuteri					658:664	reuteri	658:664	reuteri	658:664	Pretreatment with L. reuteri I5007 effectively relieves inflammation in HT-29 cells challenged with lipopolysaccharide.
32751784	2	56	dep	L.	330:331	arg1	reuteri					333:339	reuteri	333:339	reuteri	333:339	We examined whether L. reuteri I5007 could protect mice against colitis in ameliorating inflammation, modulating microbiota, and metabolic composition.
32751784	8	57	theme	immune	1478:1483	arg1	responses					1485:1493	immune responses	1478:1493	immune responses	1478:1493	Overall, the results demonstrate that L. reuteri I5007 pretreatment could effectively alleviate intestinal inflammation by regulating immune responses and altering the composition of gut microbiota structure and function, as well as improving metabolic disorders in mice with colitis.
32751784	8	58	with	mice	1610:1613	arg1	colitis					1620:1626	colitis	1620:1626	colitis	1620:1626	Overall, the results demonstrate that L. reuteri I5007 pretreatment could effectively alleviate intestinal inflammation by regulating immune responses and altering the composition of gut microbiota structure and function, as well as improving metabolic disorders in mice with colitis.
32751784	7	59	theme	colonic	1272:1278	arg1	microbiota					1280:1289	colonic microbiota	1272:1289	colonic microbiota	1272:1289	Analysis of 16S rDNA sequences and metabolome demonstrates that L. reuteri I5007 significantly alters colonic microbiota and metabolic structural and functional composition.
32751784	7	60	theme	metabolic	1295:1303	arg1	composition					1331:1341	metabolic structural and functional composition	1295:1341	metabolic structural and functional composition	1295:1341	Analysis of 16S rDNA sequences and metabolome demonstrates that L. reuteri I5007 significantly alters colonic microbiota and metabolic structural and functional composition.
32751784	6	61	theme	weight	1022:1027	arg1	loss					1029:1032	reduced weight loss	1014:1032	reduced weight loss	1014:1032	L. reuteri I5007 improved DSS-induced colitis, which was confirmed by reduced weight loss, colon length shortening, and histopathological damage, restored the mucus layer, as well as reduced pro-inflammatory cytokines levels.
32751784	1	62	contain	has	218:220	arg2	benefits					237:244	various health benefits	222:244	various health benefits including the regulation of immunity and intestinal microbiota	222:307	Lactobacillus reuteri, a commensal intestinal bacteria, has various health benefits including the regulation of immunity and intestinal microbiota.
32751784	1	62	contain	has	218:220	arg1	Lactobacillus					162:174	Lactobacillus	162:174	Lactobacillus	162:174	Lactobacillus reuteri, a commensal intestinal bacteria, has various health benefits including the regulation of immunity and intestinal microbiota.
32751784	1	62	contain	has	218:220	arg2	regulation					260:269	the regulation	256:269	the regulation of immunity and intestinal microbiota	256:307	Lactobacillus reuteri, a commensal intestinal bacteria, has various health benefits including the regulation of immunity and intestinal microbiota.
32751784	8	63	dep	L.	1382:1383	arg1	reuteri					1385:1391	reuteri	1385:1391	reuteri	1385:1391	Overall, the results demonstrate that L. reuteri I5007 pretreatment could effectively alleviate intestinal inflammation by regulating immune responses and altering the composition of gut microbiota structure and function, as well as improving metabolic disorders in mice with colitis.
32751784	6	64	theme	mucus	1103:1107	arg1	layer					1109:1113	the mucus layer	1099:1113	the mucus layer	1099:1113	L. reuteri I5007 improved DSS-induced colitis, which was confirmed by reduced weight loss, colon length shortening, and histopathological damage, restored the mucus layer, as well as reduced pro-inflammatory cytokines levels.
32751784	6	65	theme	histopathological	1064:1080	arg1	damage					1082:1087	histopathological damage	1064:1087	histopathological damage	1064:1087	L. reuteri I5007 improved DSS-induced colitis, which was confirmed by reduced weight loss, colon length shortening, and histopathological damage, restored the mucus layer, as well as reduced pro-inflammatory cytokines levels.
32751784	6	66	theme	reduced	1014:1020	arg1	loss					1029:1032	reduced weight loss	1014:1032	reduced weight loss	1014:1032	L. reuteri I5007 improved DSS-induced colitis, which was confirmed by reduced weight loss, colon length shortening, and histopathological damage, restored the mucus layer, as well as reduced pro-inflammatory cytokines levels.
32751784	7	67	theme	L.	1234:1235	arg1	I5007					1245:1249	L. reuteri I5007	1234:1249	L. reuteri I5007	1234:1249	Analysis of 16S rDNA sequences and metabolome demonstrates that L. reuteri I5007 significantly alters colonic microbiota and metabolic structural and functional composition.
32751784	7	68	theme	16S	1182:1184	arg1	sequences					1191:1199	16S rDNA sequences	1182:1199	16S rDNA sequences	1182:1199	Analysis of 16S rDNA sequences and metabolome demonstrates that L. reuteri I5007 significantly alters colonic microbiota and metabolic structural and functional composition.
32751784	3	69	dep	L.	503:504	arg1	reuteri					506:512	reuteri	506:512	reuteri	506:512	In vitro, HT-29 cells were cultured with L. reuteri I5007 or lipopolysaccharide treatment under three different conditions, i.e., pre-, co- (simultaneous), and posttreatment.
32751784	3	70	theme	different	564:572	arg1	conditions					574:583	three different conditions	558:583	three different conditions	558:583	In vitro, HT-29 cells were cultured with L. reuteri I5007 or lipopolysaccharide treatment under three different conditions, i.e., pre-, co- (simultaneous), and posttreatment.
32751784	3	70	theme	different	564:572	arg1	co-					598:600	co-	598:600	co-	598:600	In vitro, HT-29 cells were cultured with L. reuteri I5007 or lipopolysaccharide treatment under three different conditions, i.e., pre-, co- (simultaneous), and posttreatment.
32751784	3	70	theme	different	564:572	arg1	posttreatment					622:634	posttreatment	622:634	posttreatment	622:634	In vitro, HT-29 cells were cultured with L. reuteri I5007 or lipopolysaccharide treatment under three different conditions, i.e., pre-, co- (simultaneous), and posttreatment.
32751784	3	70	theme	different	564:572	arg1	pre-					592:595	pre-	592:595	pre-	592:595	In vitro, HT-29 cells were cultured with L. reuteri I5007 or lipopolysaccharide treatment under three different conditions, i.e., pre-, co- (simultaneous), and posttreatment.
32751784	0	71	from	Colitis	145:151	arg1	Mice					156:159	Mice	156:159	Mice	156:159	Lactobacillus reuteri Ameliorates Intestinal Inflammation and Modulates Gut Microbiota and Metabolic Disorders in Dextran Sulfate Sodium-Induced Colitis in Mice.
33932519	6	0	theme	positive	1045:1052	arg1	control					1054:1060	positive control	1045:1060	positive control	1045:1060	MATERIALS AND METHODS The animals were divided into different experimental groups: normal hyperglycemic (negative control); MIA (treatment of Maytenus ilicifolia extract from access 116); MIB (treatment with Maytenus ilicifolia extract from access 122; and glipizide (positive control).
33932519	6	0	theme	positive	1045:1052	arg1	glipizide					1034:1042	glipizide	1034:1042	glipizide (positive control)	1034:1061	MATERIALS AND METHODS The animals were divided into different experimental groups: normal hyperglycemic (negative control); MIA (treatment of Maytenus ilicifolia extract from access 116); MIB (treatment with Maytenus ilicifolia extract from access 122; and glipizide (positive control).
33932519	12	1	from	differences	2113:2123	arg1	composition					2137:2147	chemical composition	2128:2147	chemical composition	2128:2147	CONCLUSIONS Our data suggest that Maytenus ilicifolia extracts from different locations showed differences in chemical composition which did not reflect significant differences in the results of biological tests.
33932519	3	2	theme	ex	228:229	arg1	"					293:293	"espinheira-santa"	276:293	"espinheira-santa"	276:293	ex Reissek, Celastraceae, is popularly known as "espinheira-santa" and used to treat pathologies related to the stomach.
33932519	3	2	theme	ex	228:229	arg1	Celastraceae					240:251	Celastraceae	240:251	Celastraceae	240:251	ex Reissek, Celastraceae, is popularly known as "espinheira-santa" and used to treat pathologies related to the stomach.
33932519	3	2	theme	ex	228:229	arg1	Reissek					231:237	ex Reissek	228:237	ex Reissek	228:237	ex Reissek, Celastraceae, is popularly known as "espinheira-santa" and used to treat pathologies related to the stomach.
33932519	5	3	dep	AIM	557:559	arg1	aims					584:587	aims	584:587	aims to investigate the anti-hyperglycemic potential of ethanolic extracts obtained from leaves from two different accessions of Maytenus ilicifolia (MIA and MIB) in normal hyperglycemic rats	584:774	AIM OF THE STUDY This work aims to investigate the anti-hyperglycemic potential of ethanolic extracts obtained from leaves from two different accessions of Maytenus ilicifolia (MIA and MIB) in normal hyperglycemic rats.
33932519	6	4	dep	MIA	901:903	arg1	treatment					906:914	treatment	906:914	treatment of Maytenus ilicifolia extract from access 116	906:961	MATERIALS AND METHODS The animals were divided into different experimental groups: normal hyperglycemic (negative control); MIA (treatment of Maytenus ilicifolia extract from access 116); MIB (treatment with Maytenus ilicifolia extract from access 122; and glipizide (positive control).
33932519	5	5	theme	extracts	650:657	arg1	potential					627:635	the anti-hyperglycemic potential	604:635	the anti-hyperglycemic potential of ethanolic extracts obtained from leaves from two different accessions of Maytenus ilicifolia (MIA and MIB) in normal hyperglycemic rats	604:774	AIM OF THE STUDY This work aims to investigate the anti-hyperglycemic potential of ethanolic extracts obtained from leaves from two different accessions of Maytenus ilicifolia (MIA and MIB) in normal hyperglycemic rats.
33932519	9	6	theme	blood	1585:1589	arg1	levels					1599:1604	blood glucose levels	1585:1604	blood glucose levels	1585:1604	RESULTS Chromatographic analysis identified oleic and palmitic acid as the most common constituents, and both extracts of Maytenus ilicifolia caused a reduction in blood glucose levels within 60 min after administration of glucose overload when compared to the normal hyperglycemic group.
33932519	8	7	theme	Blood	1137:1141	arg1	collection					1143:1152	Blood collection	1137:1152	Blood collection	1137:1152	Blood collection occurred at different periods for the assessment of blood glucose (0, 60, 90 and 210 min after treatment) and at the end of the experiment blood was collected through cardiac puncture and the liver, muscle, pancreas and intestine were dissected for further analysis.
33932519	6	8	theme	normal	860:865	arg1	hyperglycemic					867:879	normal hyperglycemic	860:879	normal hyperglycemic (negative control)	860:898	MATERIALS AND METHODS The animals were divided into different experimental groups: normal hyperglycemic (negative control); MIA (treatment of Maytenus ilicifolia extract from access 116); MIB (treatment with Maytenus ilicifolia extract from access 122; and glipizide (positive control).
33932519	6	8	theme	normal	860:865	arg1	control					891:897	negative control	882:897	negative control	882:897	MATERIALS AND METHODS The animals were divided into different experimental groups: normal hyperglycemic (negative control); MIA (treatment of Maytenus ilicifolia extract from access 116); MIB (treatment with Maytenus ilicifolia extract from access 122; and glipizide (positive control).
33932519	8	9	theme	blood	1206:1210	arg1	glucose					1212:1218	blood glucose	1206:1218	blood glucose (0, 60, 90 and 210 min after treatment)	1206:1258	Blood collection occurred at different periods for the assessment of blood glucose (0, 60, 90 and 210 min after treatment) and at the end of the experiment blood was collected through cardiac puncture and the liver, muscle, pancreas and intestine were dissected for further analysis.
33932519	5	10	theme	anti-hyperglycemic	608:625	arg1	potential					627:635	the anti-hyperglycemic potential	604:635	the anti-hyperglycemic potential of ethanolic extracts obtained from leaves from two different accessions of Maytenus ilicifolia (MIA and MIB) in normal hyperglycemic rats	604:774	AIM OF THE STUDY This work aims to investigate the anti-hyperglycemic potential of ethanolic extracts obtained from leaves from two different accessions of Maytenus ilicifolia (MIA and MIB) in normal hyperglycemic rats.
33932519	6	11	from	access	1018:1023	arg1	extract					1005:1011	Maytenus ilicifolia extract	985:1011	Maytenus ilicifolia extract from access 122	985:1027	MATERIALS AND METHODS The animals were divided into different experimental groups: normal hyperglycemic (negative control); MIA (treatment of Maytenus ilicifolia extract from access 116); MIB (treatment with Maytenus ilicifolia extract from access 122; and glipizide (positive control).
33932519	11	12	theme	triglyceride	1977:1988	arg1	levels					2010:2015	triglyceride and HDL cholesterol levels	1977:2015	triglyceride and HDL cholesterol levels	1977:2015	However, hyperglycemic rats treated with the extracts showed a marked increase in triglyceride and HDL cholesterol levels.
33932519	3	13	theme	espinheira-santa	277:292	arg1	"					293:293	"espinheira-santa"	276:293	"espinheira-santa"	276:293	ex Reissek, Celastraceae, is popularly known as "espinheira-santa" and used to treat pathologies related to the stomach.
33932519	3	13	theme	espinheira-santa	277:292	arg1	Reissek					231:237	ex Reissek	228:237	ex Reissek	228:237	ex Reissek, Celastraceae, is popularly known as "espinheira-santa" and used to treat pathologies related to the stomach.
33932519	6	14	dep	MATERIALS	777:785	arg1	animals					803:809	The animals	799:809	The animals	799:809	MATERIALS AND METHODS The animals were divided into different experimental groups: normal hyperglycemic (negative control); MIA (treatment of Maytenus ilicifolia extract from access 116); MIB (treatment with Maytenus ilicifolia extract from access 122; and glipizide (positive control).
33932519	5	15	theme	different	689:697	arg1	accessions					699:708	two different accessions	685:708	two different accessions of Maytenus ilicifolia (MIA and MIB)	685:745	AIM OF THE STUDY This work aims to investigate the anti-hyperglycemic potential of ethanolic extracts obtained from leaves from two different accessions of Maytenus ilicifolia (MIA and MIB) in normal hyperglycemic rats.
33932519	8	16	theme	different	1166:1174	arg1	periods					1176:1182	different periods	1166:1182	different periods for the assessment of blood glucose (0, 60, 90 and 210 min after treatment)	1166:1258	Blood collection occurred at different periods for the assessment of blood glucose (0, 60, 90 and 210 min after treatment) and at the end of the experiment blood was collected through cardiac puncture and the liver, muscle, pancreas and intestine were dissected for further analysis.
33932519	6	17	theme	ilicifolia	928:937	arg1	extract					939:945	Maytenus ilicifolia extract	919:945	Maytenus ilicifolia extract from access 116	919:961	MATERIALS AND METHODS The animals were divided into different experimental groups: normal hyperglycemic (negative control); MIA (treatment of Maytenus ilicifolia extract from access 116); MIB (treatment with Maytenus ilicifolia extract from access 122; and glipizide (positive control).
33932519	4	18	theme	other	419:423	arg1	disorders					425:433	other disorders	419:433	other disorders such as diabetes, but without scientific evidence	419:483	However, in popular culture, this species has also been used to treat other disorders such as diabetes, but without scientific evidence, requiring more phytochemical and pharmacological studies on the plant.
33932519	6	19	with	treatment	970:978	arg1	extract					1005:1011	Maytenus ilicifolia extract	985:1011	Maytenus ilicifolia extract from access 122	985:1027	MATERIALS AND METHODS The animals were divided into different experimental groups: normal hyperglycemic (negative control); MIA (treatment of Maytenus ilicifolia extract from access 116); MIB (treatment with Maytenus ilicifolia extract from access 122; and glipizide (positive control).
33932519	12	20	theme	ilicifolia	2061:2070	arg1	extracts					2072:2079	Maytenus ilicifolia extracts	2052:2079	Maytenus ilicifolia extracts from different locations	2052:2104	CONCLUSIONS Our data suggest that Maytenus ilicifolia extracts from different locations showed differences in chemical composition which did not reflect significant differences in the results of biological tests.
33932519	13	21	theme	new	2454:2456	arg1	studies					2458:2464	new studies	2454:2464	new studies using different technologies in order	2454:2502	In addition, it was possible to conclude that the treatment with Maytenus ilicifolia had a discreet anti-hyperglycemic effect; however, it was not possible to identify the responsible mechanism, being necessary, therefore, new studies using different technologies in order to determine the possible mechanisms of action of the extract.
33932519	9	22	theme	glucose	1644:1650	arg1	overload					1652:1659	glucose overload	1644:1659	glucose overload	1644:1659	RESULTS Chromatographic analysis identified oleic and palmitic acid as the most common constituents, and both extracts of Maytenus ilicifolia caused a reduction in blood glucose levels within 60 min after administration of glucose overload when compared to the normal hyperglycemic group.
33932519	10	23	theme	muscular	1762:1769	arg1	levels					1780:1785	muscular glycogen levels	1762:1785	muscular glycogen levels	1762:1785	No significant changes were observed in hepatic and muscular glycogen levels, plasma insulin concentration and disaccharidases activity with none of the extracts in the model employed.
33932519	1	24	theme	glucose	122:128	arg1	metabolism					130:139	glucose metabolism	122:139	glucose metabolism in normal hyperglycemic rats	122:168	ex Reissek on glucose metabolism in normal hyperglycemic rats.
33932519	7	25	theme	glucose	1112:1118	arg1	overload					1120:1127	glucose overload	1112:1127	glucose overload	1112:1127	At 30 min after treatment, all animals received glucose overload orally.
33932519	9	26	theme	Chromatographic	1429:1443	arg1	analysis					1445:1452	RESULTS Chromatographic analysis	1421:1452	RESULTS Chromatographic analysis	1421:1452	RESULTS Chromatographic analysis identified oleic and palmitic acid as the most common constituents, and both extracts of Maytenus ilicifolia caused a reduction in blood glucose levels within 60 min after administration of glucose overload when compared to the normal hyperglycemic group.
33932519	9	27	theme	normal	1682:1687	arg1	group					1703:1707	the normal hyperglycemic group	1678:1707	the normal hyperglycemic group	1678:1707	RESULTS Chromatographic analysis identified oleic and palmitic acid as the most common constituents, and both extracts of Maytenus ilicifolia caused a reduction in blood glucose levels within 60 min after administration of glucose overload when compared to the normal hyperglycemic group.
33932519	4	28	theme	scientific	465:474	arg1	evidence					476:483	scientific evidence	465:483	scientific evidence	465:483	However, in popular culture, this species has also been used to treat other disorders such as diabetes, but without scientific evidence, requiring more phytochemical and pharmacological studies on the plant.
33932519	0	29	theme	extracts	70:77	arg1	Characterization					0:15	Characterization	0:15	Characterization of the chemical profile	0:39	Characterization of the chemical profile and the effects of ethanolic extracts of Maytenus ilicifolia Mart.
33932519	0	29	theme	extracts	70:77	arg1	effects					49:55	the effects	45:55	the effects of ethanolic extracts of Maytenus ilicifolia Mart	45:105	Characterization of the chemical profile and the effects of ethanolic extracts of Maytenus ilicifolia Mart.
33932519	5	30	theme	STUDY	568:572	arg1	AIM					557:559	AIM	557:559	AIM OF THE STUDY This work aims to investigate the anti-hyperglycemic potential of ethanolic extracts obtained from leaves from two different accessions of Maytenus ilicifolia (MIA and MIB) in normal hyperglycemic rats.	557:775	AIM OF THE STUDY This work aims to investigate the anti-hyperglycemic potential of ethanolic extracts obtained from leaves from two different accessions of Maytenus ilicifolia (MIA and MIB) in normal hyperglycemic rats.
33932519	1	31	theme	hyperglycemic	151:163	arg1	rats					165:168	normal hyperglycemic rats	144:168	normal hyperglycemic rats	144:168	ex Reissek on glucose metabolism in normal hyperglycemic rats.
33932519	5	32	theme	ilicifolia	722:731	arg1	accessions					699:708	two different accessions	685:708	two different accessions of Maytenus ilicifolia (MIA and MIB)	685:745	AIM OF THE STUDY This work aims to investigate the anti-hyperglycemic potential of ethanolic extracts obtained from leaves from two different accessions of Maytenus ilicifolia (MIA and MIB) in normal hyperglycemic rats.
33932519	1	33	from	metabolism	130:139	arg1	rats					165:168	normal hyperglycemic rats	144:168	normal hyperglycemic rats	144:168	ex Reissek on glucose metabolism in normal hyperglycemic rats.
33932519	12	34	theme	tests	2224:2228	arg1	results					2202:2208	the results	2198:2208	the results of biological tests	2198:2228	CONCLUSIONS Our data suggest that Maytenus ilicifolia extracts from different locations showed differences in chemical composition which did not reflect significant differences in the results of biological tests.
33932519	12	35	theme	biological	2213:2222	arg1	tests					2224:2228	biological tests	2213:2228	biological tests	2213:2228	CONCLUSIONS Our data suggest that Maytenus ilicifolia extracts from different locations showed differences in chemical composition which did not reflect significant differences in the results of biological tests.
33932519	0	36	theme	Mart	102:105	arg1	extracts					70:77	ethanolic extracts	60:77	ethanolic extracts of Maytenus ilicifolia Mart	60:105	Characterization of the chemical profile and the effects of ethanolic extracts of Maytenus ilicifolia Mart.
33932519	10	37	located	observed	1738:1745	arg2	changes					1725:1731	No significant changes	1710:1731	No significant changes	1710:1731	No significant changes were observed in hepatic and muscular glycogen levels, plasma insulin concentration and disaccharidases activity with none of the extracts in the model employed.
33932519	10	37	located	observed	1738:1745	arg1	levels					1780:1785	muscular glycogen levels	1762:1785	muscular glycogen levels	1762:1785	No significant changes were observed in hepatic and muscular glycogen levels, plasma insulin concentration and disaccharidases activity with none of the extracts in the model employed.
33932519	10	37	located	observed	1738:1745	arg1	hepatic					1750:1756	hepatic	1750:1756	hepatic	1750:1756	No significant changes were observed in hepatic and muscular glycogen levels, plasma insulin concentration and disaccharidases activity with none of the extracts in the model employed.
33932519	10	37	located	observed	1738:1745	arg1	model					1879:1883	the model	1875:1883	the model employed	1875:1892	No significant changes were observed in hepatic and muscular glycogen levels, plasma insulin concentration and disaccharidases activity with none of the extracts in the model employed.
33932519	8	38	dep	glucose	1212:1218	arg1	210 min					1235:1241	0, 60, 90 and 210 min	1221:1241	0, 60, 90 and 210 min	1221:1241	Blood collection occurred at different periods for the assessment of blood glucose (0, 60, 90 and 210 min after treatment) and at the end of the experiment blood was collected through cardiac puncture and the liver, muscle, pancreas and intestine were dissected for further analysis.
33932519	12	39	theme	significant	2171:2181	arg1	differences					2183:2193	significant differences	2171:2193	significant differences in the results of biological tests	2171:2228	CONCLUSIONS Our data suggest that Maytenus ilicifolia extracts from different locations showed differences in chemical composition which did not reflect significant differences in the results of biological tests.
33932519	10	40	theme	plasma	1788:1793	arg1	concentration					1803:1815	plasma insulin concentration	1788:1815	plasma insulin concentration	1788:1815	No significant changes were observed in hepatic and muscular glycogen levels, plasma insulin concentration and disaccharidases activity with none of the extracts in the model employed.
33932519	8	41	theme	blood	1293:1297	arg1	end					1271:1273	the end	1267:1273	the end of the experiment blood	1267:1297	Blood collection occurred at different periods for the assessment of blood glucose (0, 60, 90 and 210 min after treatment) and at the end of the experiment blood was collected through cardiac puncture and the liver, muscle, pancreas and intestine were dissected for further analysis.
33932519	4	42	from	studies	535:541	arg1	plant					550:554	the plant	546:554	the plant	546:554	However, in popular culture, this species has also been used to treat other disorders such as diabetes, but without scientific evidence, requiring more phytochemical and pharmacological studies on the plant.
33932519	9	43	theme	common	1501:1506	arg1	constituents					1508:1519	the most common constituents	1492:1519	the most common constituents	1492:1519	RESULTS Chromatographic analysis identified oleic and palmitic acid as the most common constituents, and both extracts of Maytenus ilicifolia caused a reduction in blood glucose levels within 60 min after administration of glucose overload when compared to the normal hyperglycemic group.
33932519	9	43	theme	common	1501:1506	arg1	acid					1484:1487	oleic and palmitic acid	1465:1487	oleic and palmitic acid	1465:1487	RESULTS Chromatographic analysis identified oleic and palmitic acid as the most common constituents, and both extracts of Maytenus ilicifolia caused a reduction in blood glucose levels within 60 min after administration of glucose overload when compared to the normal hyperglycemic group.
33932519	6	44	from	access	952:957	arg1	extract					939:945	Maytenus ilicifolia extract	919:945	Maytenus ilicifolia extract from access 116	919:961	MATERIALS AND METHODS The animals were divided into different experimental groups: normal hyperglycemic (negative control); MIA (treatment of Maytenus ilicifolia extract from access 116); MIB (treatment with Maytenus ilicifolia extract from access 122; and glipizide (positive control).
33932519	6	44	from	access	952:957	arg1	treatment					906:914	treatment	906:914	treatment of Maytenus ilicifolia extract from access 116	906:961	MATERIALS AND METHODS The animals were divided into different experimental groups: normal hyperglycemic (negative control); MIA (treatment of Maytenus ilicifolia extract from access 116); MIB (treatment with Maytenus ilicifolia extract from access 122; and glipizide (positive control).
33932519	2	45	theme	ETHNOPHARMACOLOGICAL	171:190	arg1	RELEVANCE					192:200	ETHNOPHARMACOLOGICAL RELEVANCE	171:200	ETHNOPHARMACOLOGICAL RELEVANCE	171:200	ETHNOPHARMACOLOGICAL RELEVANCE Maytenus ilicifolia Mart.
33932519	0	46	theme	profile	33:39	arg1	Characterization					0:15	Characterization	0:15	Characterization of the chemical profile	0:39	Characterization of the chemical profile and the effects of ethanolic extracts of Maytenus ilicifolia Mart.
33932519	0	46	theme	profile	33:39	arg1	effects					49:55	the effects	45:55	the effects of ethanolic extracts of Maytenus ilicifolia Mart	45:105	Characterization of the chemical profile and the effects of ethanolic extracts of Maytenus ilicifolia Mart.
33932519	5	47	theme	hyperglycemic	757:769	arg1	rats					771:774	normal hyperglycemic rats	750:774	normal hyperglycemic rats	750:774	AIM OF THE STUDY This work aims to investigate the anti-hyperglycemic potential of ethanolic extracts obtained from leaves from two different accessions of Maytenus ilicifolia (MIA and MIB) in normal hyperglycemic rats.
33932519	6	48	theme	experimental	839:850	arg1	groups					852:857	different experimental groups	829:857	different experimental groups	829:857	MATERIALS AND METHODS The animals were divided into different experimental groups: normal hyperglycemic (negative control); MIA (treatment of Maytenus ilicifolia extract from access 116); MIB (treatment with Maytenus ilicifolia extract from access 122; and glipizide (positive control).
33932519	8	49	dep	occurred	1154:1161	arg1	collected					1303:1311	collected	1303:1311	was collected through cardiac puncture	1299:1336	Blood collection occurred at different periods for the assessment of blood glucose (0, 60, 90 and 210 min after treatment) and at the end of the experiment blood was collected through cardiac puncture and the liver, muscle, pancreas and intestine were dissected for further analysis.
33932519	8	50	theme	cardiac	1321:1327	arg1	puncture					1329:1336	cardiac puncture	1321:1336	cardiac puncture	1321:1336	Blood collection occurred at different periods for the assessment of blood glucose (0, 60, 90 and 210 min after treatment) and at the end of the experiment blood was collected through cardiac puncture and the liver, muscle, pancreas and intestine were dissected for further analysis.
33932519	5	51	from	potential	627:635	arg1	rats					771:774	normal hyperglycemic rats	750:774	normal hyperglycemic rats	750:774	AIM OF THE STUDY This work aims to investigate the anti-hyperglycemic potential of ethanolic extracts obtained from leaves from two different accessions of Maytenus ilicifolia (MIA and MIB) in normal hyperglycemic rats.
33932519	6	52	with	glipizide	1034:1042	arg1	extract					1005:1011	Maytenus ilicifolia extract	985:1011	Maytenus ilicifolia extract from access 122	985:1027	MATERIALS AND METHODS The animals were divided into different experimental groups: normal hyperglycemic (negative control); MIA (treatment of Maytenus ilicifolia extract from access 116); MIB (treatment with Maytenus ilicifolia extract from access 122; and glipizide (positive control).
33932519	12	53	theme	chemical	2128:2135	arg1	composition					2137:2147	chemical composition	2128:2147	chemical composition	2128:2147	CONCLUSIONS Our data suggest that Maytenus ilicifolia extracts from different locations showed differences in chemical composition which did not reflect significant differences in the results of biological tests.
33932519	9	54	theme	ilicifolia	1552:1561	arg1	extracts					1531:1538	both extracts	1526:1538	both extracts of Maytenus ilicifolia	1526:1561	RESULTS Chromatographic analysis identified oleic and palmitic acid as the most common constituents, and both extracts of Maytenus ilicifolia caused a reduction in blood glucose levels within 60 min after administration of glucose overload when compared to the normal hyperglycemic group.
33932519	2	55	dep	Mart	222:225	arg1	RELEVANCE					192:200	ETHNOPHARMACOLOGICAL RELEVANCE	171:200	ETHNOPHARMACOLOGICAL RELEVANCE	171:200	ETHNOPHARMACOLOGICAL RELEVANCE Maytenus ilicifolia Mart.
33932519	6	56	from	treatment	906:914	arg1	access					952:957	access 116	952:961	access 116	952:961	MATERIALS AND METHODS The animals were divided into different experimental groups: normal hyperglycemic (negative control); MIA (treatment of Maytenus ilicifolia extract from access 116); MIB (treatment with Maytenus ilicifolia extract from access 122; and glipizide (positive control).
33932519	10	57	dep	levels	1780:1785	arg1	activity					1837:1844	activity	1837:1844	activity	1837:1844	No significant changes were observed in hepatic and muscular glycogen levels, plasma insulin concentration and disaccharidases activity with none of the extracts in the model employed.
33932519	5	58	theme	ethanolic	640:648	arg1	extracts					650:657	ethanolic extracts	640:657	ethanolic extracts obtained from leaves from two different accessions of Maytenus ilicifolia (MIA and MIB)	640:745	AIM OF THE STUDY This work aims to investigate the anti-hyperglycemic potential of ethanolic extracts obtained from leaves from two different accessions of Maytenus ilicifolia (MIA and MIB) in normal hyperglycemic rats.
33932519	13	59	from	addition	2234:2241	arg1	possible					2251:2258	possible	2251:2258	possible	2251:2258	In addition, it was possible to conclude that the treatment with Maytenus ilicifolia had a discreet anti-hyperglycemic effect; however, it was not possible to identify the responsible mechanism, being necessary, therefore, new studies using different technologies in order to determine the possible mechanisms of action of the extract.
33932519	6	60	theme	negative	882:889	arg1	hyperglycemic					867:879	normal hyperglycemic	860:879	normal hyperglycemic (negative control)	860:898	MATERIALS AND METHODS The animals were divided into different experimental groups: normal hyperglycemic (negative control); MIA (treatment of Maytenus ilicifolia extract from access 116); MIB (treatment with Maytenus ilicifolia extract from access 122; and glipizide (positive control).
33932519	6	60	theme	negative	882:889	arg1	control					891:897	negative control	882:897	negative control	882:897	MATERIALS AND METHODS The animals were divided into different experimental groups: normal hyperglycemic (negative control); MIA (treatment of Maytenus ilicifolia extract from access 116); MIB (treatment with Maytenus ilicifolia extract from access 122; and glipizide (positive control).
33932519	13	61	contain	had	2316:2318	arg2	effect					2350:2355	a discreet anti-hyperglycemic effect	2320:2355	a discreet anti-hyperglycemic effect	2320:2355	In addition, it was possible to conclude that the treatment with Maytenus ilicifolia had a discreet anti-hyperglycemic effect; however, it was not possible to identify the responsible mechanism, being necessary, therefore, new studies using different technologies in order to determine the possible mechanisms of action of the extract.
33932519	13	61	contain	had	2316:2318	arg1	treatment					2281:2289	the treatment	2277:2289	the treatment with Maytenus ilicifolia	2277:2314	In addition, it was possible to conclude that the treatment with Maytenus ilicifolia had a discreet anti-hyperglycemic effect; however, it was not possible to identify the responsible mechanism, being necessary, therefore, new studies using different technologies in order to determine the possible mechanisms of action of the extract.
33932519	9	62	theme	glucose	1591:1597	arg1	levels					1599:1604	blood glucose levels	1585:1604	blood glucose levels	1585:1604	RESULTS Chromatographic analysis identified oleic and palmitic acid as the most common constituents, and both extracts of Maytenus ilicifolia caused a reduction in blood glucose levels within 60 min after administration of glucose overload when compared to the normal hyperglycemic group.
33932519	4	63	theme	popular	361:367	arg1	culture					369:375	popular culture	361:375	popular culture	361:375	However, in popular culture, this species has also been used to treat other disorders such as diabetes, but without scientific evidence, requiring more phytochemical and pharmacological studies on the plant.
33932519	3	64	theme	related	325:331	arg1	pathologies					313:323	pathologies	313:323	pathologies related to the stomach	313:346	ex Reissek, Celastraceae, is popularly known as "espinheira-santa" and used to treat pathologies related to the stomach.
33932519	13	65	theme	different	2472:2480	arg1	technologies					2482:2493	different technologies	2472:2493	different technologies	2472:2493	In addition, it was possible to conclude that the treatment with Maytenus ilicifolia had a discreet anti-hyperglycemic effect; however, it was not possible to identify the responsible mechanism, being necessary, therefore, new studies using different technologies in order to determine the possible mechanisms of action of the extract.
33932519	13	66	from	possible	2251:2258	arg1	addition					2234:2241	addition	2234:2241	addition	2234:2241	In addition, it was possible to conclude that the treatment with Maytenus ilicifolia had a discreet anti-hyperglycemic effect; however, it was not possible to identify the responsible mechanism, being necessary, therefore, new studies using different technologies in order to determine the possible mechanisms of action of the extract.
33932519	10	67	theme	significant	1713:1723	arg1	changes					1725:1731	No significant changes	1710:1731	No significant changes	1710:1731	No significant changes were observed in hepatic and muscular glycogen levels, plasma insulin concentration and disaccharidases activity with none of the extracts in the model employed.
33932519	13	68	theme	Maytenus	2296:2303	arg1	ilicifolia					2305:2314	Maytenus ilicifolia	2296:2314	Maytenus ilicifolia	2296:2314	In addition, it was possible to conclude that the treatment with Maytenus ilicifolia had a discreet anti-hyperglycemic effect; however, it was not possible to identify the responsible mechanism, being necessary, therefore, new studies using different technologies in order to determine the possible mechanisms of action of the extract.
33932519	12	69	theme	different	2086:2094	arg1	locations					2096:2104	different locations	2086:2104	different locations	2086:2104	CONCLUSIONS Our data suggest that Maytenus ilicifolia extracts from different locations showed differences in chemical composition which did not reflect significant differences in the results of biological tests.
33932519	11	70	theme	marked	1958:1963	arg1	increase					1965:1972	a marked increase	1956:1972	a marked increase in triglyceride and HDL cholesterol levels	1956:2015	However, hyperglycemic rats treated with the extracts showed a marked increase in triglyceride and HDL cholesterol levels.
33932519	12	71	from	locations	2096:2104	arg1	extracts					2072:2079	Maytenus ilicifolia extracts	2052:2079	Maytenus ilicifolia extracts from different locations	2052:2104	CONCLUSIONS Our data suggest that Maytenus ilicifolia extracts from different locations showed differences in chemical composition which did not reflect significant differences in the results of biological tests.
33932519	6	72	theme	extract	939:945	arg1	treatment					906:914	treatment	906:914	treatment of Maytenus ilicifolia extract from access 116	906:961	MATERIALS AND METHODS The animals were divided into different experimental groups: normal hyperglycemic (negative control); MIA (treatment of Maytenus ilicifolia extract from access 116); MIB (treatment with Maytenus ilicifolia extract from access 122; and glipizide (positive control).
33932519	8	73	theme	glucose	1212:1218	arg1	assessment					1192:1201	the assessment	1188:1201	the assessment of blood glucose (0, 60, 90 and 210 min after treatment)	1188:1258	Blood collection occurred at different periods for the assessment of blood glucose (0, 60, 90 and 210 min after treatment) and at the end of the experiment blood was collected through cardiac puncture and the liver, muscle, pancreas and intestine were dissected for further analysis.
33932519	9	74	from	reduction	1572:1580	arg1	levels					1599:1604	blood glucose levels	1585:1604	blood glucose levels	1585:1604	RESULTS Chromatographic analysis identified oleic and palmitic acid as the most common constituents, and both extracts of Maytenus ilicifolia caused a reduction in blood glucose levels within 60 min after administration of glucose overload when compared to the normal hyperglycemic group.
33932519	11	75	theme	HDL	1994:1996	arg1	cholesterol					1998:2008	HDL cholesterol	1994:2008	HDL cholesterol	1994:2008	However, hyperglycemic rats treated with the extracts showed a marked increase in triglyceride and HDL cholesterol levels.
33932519	12	76	theme	Maytenus	2052:2059	arg1	extracts					2072:2079	Maytenus ilicifolia extracts	2052:2079	Maytenus ilicifolia extracts from different locations	2052:2104	CONCLUSIONS Our data suggest that Maytenus ilicifolia extracts from different locations showed differences in chemical composition which did not reflect significant differences in the results of biological tests.
33932519	9	77	theme	overload	1652:1659	arg1	administration					1626:1639	administration	1626:1639	administration of glucose overload	1626:1659	RESULTS Chromatographic analysis identified oleic and palmitic acid as the most common constituents, and both extracts of Maytenus ilicifolia caused a reduction in blood glucose levels within 60 min after administration of glucose overload when compared to the normal hyperglycemic group.
33932519	10	78	theme	glycogen	1771:1778	arg1	levels					1780:1785	muscular glycogen levels	1762:1785	muscular glycogen levels	1762:1785	No significant changes were observed in hepatic and muscular glycogen levels, plasma insulin concentration and disaccharidases activity with none of the extracts in the model employed.
33932519	6	79	theme	Maytenus	985:992	arg1	extract					1005:1011	Maytenus ilicifolia extract	985:1011	Maytenus ilicifolia extract from access 122	985:1027	MATERIALS AND METHODS The animals were divided into different experimental groups: normal hyperglycemic (negative control); MIA (treatment of Maytenus ilicifolia extract from access 116); MIB (treatment with Maytenus ilicifolia extract from access 122; and glipizide (positive control).
33932519	5	80	dep	ilicifolia	722:731	arg1	MIB					742:744	MIB	742:744	MIB	742:744	AIM OF THE STUDY This work aims to investigate the anti-hyperglycemic potential of ethanolic extracts obtained from leaves from two different accessions of Maytenus ilicifolia (MIA and MIB) in normal hyperglycemic rats.
33932519	5	80	dep	ilicifolia	722:731	arg1	MIA					734:736	MIA	734:736	MIA	734:736	AIM OF THE STUDY This work aims to investigate the anti-hyperglycemic potential of ethanolic extracts obtained from leaves from two different accessions of Maytenus ilicifolia (MIA and MIB) in normal hyperglycemic rats.
33932519	12	81	from	differences	2183:2193	arg1	results					2202:2208	the results	2198:2208	the results of biological tests	2198:2228	CONCLUSIONS Our data suggest that Maytenus ilicifolia extracts from different locations showed differences in chemical composition which did not reflect significant differences in the results of biological tests.
33932519	0	82	theme	ethanolic	60:68	arg1	extracts					70:77	ethanolic extracts	60:77	ethanolic extracts of Maytenus ilicifolia Mart	60:105	Characterization of the chemical profile and the effects of ethanolic extracts of Maytenus ilicifolia Mart.
33932519	11	83	theme	cholesterol	1998:2008	arg1	levels					2010:2015	triglyceride and HDL cholesterol levels	1977:2015	triglyceride and HDL cholesterol levels	1977:2015	However, hyperglycemic rats treated with the extracts showed a marked increase in triglyceride and HDL cholesterol levels.
33932519	11	84	theme	hyperglycemic	1904:1916	arg1	rats					1918:1921	hyperglycemic rats	1904:1921	hyperglycemic rats treated with the extracts	1904:1947	However, hyperglycemic rats treated with the extracts showed a marked increase in triglyceride and HDL cholesterol levels.
33932519	13	85	theme	extract	2558:2564	arg1	action					2544:2549	action	2544:2549	action of the extract	2544:2564	In addition, it was possible to conclude that the treatment with Maytenus ilicifolia had a discreet anti-hyperglycemic effect; however, it was not possible to identify the responsible mechanism, being necessary, therefore, new studies using different technologies in order to determine the possible mechanisms of action of the extract.
33932519	9	86	theme	RESULTS	1421:1427	arg1	analysis					1445:1452	RESULTS Chromatographic analysis	1421:1452	RESULTS Chromatographic analysis	1421:1452	RESULTS Chromatographic analysis identified oleic and palmitic acid as the most common constituents, and both extracts of Maytenus ilicifolia caused a reduction in blood glucose levels within 60 min after administration of glucose overload when compared to the normal hyperglycemic group.
33932519	8	87	theme	further	1403:1409	arg1	analysis					1411:1418	further analysis	1403:1418	further analysis	1403:1418	Blood collection occurred at different periods for the assessment of blood glucose (0, 60, 90 and 210 min after treatment) and at the end of the experiment blood was collected through cardiac puncture and the liver, muscle, pancreas and intestine were dissected for further analysis.
33932519	1	88	theme	normal	144:149	arg1	rats					165:168	normal hyperglycemic rats	144:168	normal hyperglycemic rats	144:168	ex Reissek on glucose metabolism in normal hyperglycemic rats.
33932519	6	89	theme	MIB	965:967	arg1	treatment					970:978	MIB (treatment	965:978	MIB (treatment with Maytenus ilicifolia extract from access 122	965:1027	MATERIALS AND METHODS The animals were divided into different experimental groups: normal hyperglycemic (negative control); MIA (treatment of Maytenus ilicifolia extract from access 116); MIB (treatment with Maytenus ilicifolia extract from access 122; and glipizide (positive control).
33932519	9	90	theme	hyperglycemic	1689:1701	arg1	group					1703:1707	the normal hyperglycemic group	1678:1707	the normal hyperglycemic group	1678:1707	RESULTS Chromatographic analysis identified oleic and palmitic acid as the most common constituents, and both extracts of Maytenus ilicifolia caused a reduction in blood glucose levels within 60 min after administration of glucose overload when compared to the normal hyperglycemic group.
33932519	9	91	theme	oleic	1465:1469	arg1	constituents					1508:1519	the most common constituents	1492:1519	the most common constituents	1492:1519	RESULTS Chromatographic analysis identified oleic and palmitic acid as the most common constituents, and both extracts of Maytenus ilicifolia caused a reduction in blood glucose levels within 60 min after administration of glucose overload when compared to the normal hyperglycemic group.
33932519	9	91	theme	oleic	1465:1469	arg1	acid					1484:1487	oleic and palmitic acid	1465:1487	oleic and palmitic acid	1465:1487	RESULTS Chromatographic analysis identified oleic and palmitic acid as the most common constituents, and both extracts of Maytenus ilicifolia caused a reduction in blood glucose levels within 60 min after administration of glucose overload when compared to the normal hyperglycemic group.
33932519	13	92	dep	necessary	2432:2440	arg1	determine					2507:2515	determine	2507:2515	to determine the possible mechanisms of action of the extract	2504:2564	In addition, it was possible to conclude that the treatment with Maytenus ilicifolia had a discreet anti-hyperglycemic effect; however, it was not possible to identify the responsible mechanism, being necessary, therefore, new studies using different technologies in order to determine the possible mechanisms of action of the extract.
33932519	9	93	theme	palmitic	1475:1482	arg1	constituents					1508:1519	the most common constituents	1492:1519	the most common constituents	1492:1519	RESULTS Chromatographic analysis identified oleic and palmitic acid as the most common constituents, and both extracts of Maytenus ilicifolia caused a reduction in blood glucose levels within 60 min after administration of glucose overload when compared to the normal hyperglycemic group.
33932519	9	93	theme	palmitic	1475:1482	arg1	acid					1484:1487	oleic and palmitic acid	1465:1487	oleic and palmitic acid	1465:1487	RESULTS Chromatographic analysis identified oleic and palmitic acid as the most common constituents, and both extracts of Maytenus ilicifolia caused a reduction in blood glucose levels within 60 min after administration of glucose overload when compared to the normal hyperglycemic group.
33932519	6	94	dep	Maytenus	985:992	arg1	ilicifolia					994:1003	ilicifolia	994:1003	ilicifolia	994:1003	MATERIALS AND METHODS The animals were divided into different experimental groups: normal hyperglycemic (negative control); MIA (treatment of Maytenus ilicifolia extract from access 116); MIB (treatment with Maytenus ilicifolia extract from access 122; and glipizide (positive control).
33932519	3	95	used	used	299:302	arg2	"					293:293	"espinheira-santa"	276:293	"espinheira-santa"	276:293	ex Reissek, Celastraceae, is popularly known as "espinheira-santa" and used to treat pathologies related to the stomach.
33932519	3	95	used	used	299:302	arg2	Celastraceae					240:251	Celastraceae	240:251	Celastraceae	240:251	ex Reissek, Celastraceae, is popularly known as "espinheira-santa" and used to treat pathologies related to the stomach.
33932519	3	95	used	used	299:302	arg2	Reissek					231:237	ex Reissek	228:237	ex Reissek	228:237	ex Reissek, Celastraceae, is popularly known as "espinheira-santa" and used to treat pathologies related to the stomach.
33932519	1	96	dep	Reissek	111:117	arg1	ex					108:109	ex Reissek on glucose metabolism in normal hyperglycemic rats	108:168	ex Reissek on glucose metabolism in normal hyperglycemic rats	108:168	ex Reissek on glucose metabolism in normal hyperglycemic rats.
33932519	13	97	theme	action	2544:2549	arg1	mechanisms					2530:2539	the possible mechanisms	2517:2539	the possible mechanisms of action of the extract	2517:2564	In addition, it was possible to conclude that the treatment with Maytenus ilicifolia had a discreet anti-hyperglycemic effect; however, it was not possible to identify the responsible mechanism, being necessary, therefore, new studies using different technologies in order to determine the possible mechanisms of action of the extract.
33932519	13	98	theme	possible	2521:2528	arg1	mechanisms					2530:2539	the possible mechanisms	2517:2539	the possible mechanisms of action of the extract	2517:2564	In addition, it was possible to conclude that the treatment with Maytenus ilicifolia had a discreet anti-hyperglycemic effect; however, it was not possible to identify the responsible mechanism, being necessary, therefore, new studies using different technologies in order to determine the possible mechanisms of action of the extract.
33932519	0	99	theme	chemical	24:31	arg1	profile					33:39	the chemical profile	20:39	the chemical profile	20:39	Characterization of the chemical profile and the effects of ethanolic extracts of Maytenus ilicifolia Mart.
33932519	13	100	theme	discreet	2322:2329	arg1	effect					2350:2355	a discreet anti-hyperglycemic effect	2320:2355	a discreet anti-hyperglycemic effect	2320:2355	In addition, it was possible to conclude that the treatment with Maytenus ilicifolia had a discreet anti-hyperglycemic effect; however, it was not possible to identify the responsible mechanism, being necessary, therefore, new studies using different technologies in order to determine the possible mechanisms of action of the extract.
33932519	12	101	dep	CONCLUSIONS	2018:2028	arg1	suggest					2039:2045	suggest	2039:2045	suggest that Maytenus ilicifolia extracts from different locations showed differences in chemical composition which did not reflect significant differences in the results of biological tests	2039:2228	CONCLUSIONS Our data suggest that Maytenus ilicifolia extracts from different locations showed differences in chemical composition which did not reflect significant differences in the results of biological tests.
33932519	10	102	theme	insulin	1795:1801	arg1	concentration					1803:1815	plasma insulin concentration	1788:1815	plasma insulin concentration	1788:1815	No significant changes were observed in hepatic and muscular glycogen levels, plasma insulin concentration and disaccharidases activity with none of the extracts in the model employed.
33932519	8	103	theme	experiment	1282:1291	arg1	blood					1293:1297	the experiment blood	1278:1297	the experiment blood	1278:1297	Blood collection occurred at different periods for the assessment of blood glucose (0, 60, 90 and 210 min after treatment) and at the end of the experiment blood was collected through cardiac puncture and the liver, muscle, pancreas and intestine were dissected for further analysis.
33932519	5	104	theme	normal	750:755	arg1	rats					771:774	normal hyperglycemic rats	750:774	normal hyperglycemic rats	750:774	AIM OF THE STUDY This work aims to investigate the anti-hyperglycemic potential of ethanolic extracts obtained from leaves from two different accessions of Maytenus ilicifolia (MIA and MIB) in normal hyperglycemic rats.
33932519	4	105	theme	phytochemical	501:513	arg1	studies					535:541	more phytochemical and pharmacological studies	496:541	more phytochemical and pharmacological studies on the plant	496:554	However, in popular culture, this species has also been used to treat other disorders such as diabetes, but without scientific evidence, requiring more phytochemical and pharmacological studies on the plant.
33932519	4	106	used	used	405:408	arg2	species					383:389	this species	378:389	this species	378:389	However, in popular culture, this species has also been used to treat other disorders such as diabetes, but without scientific evidence, requiring more phytochemical and pharmacological studies on the plant.
33932519	6	107	theme	Maytenus	919:926	arg1	extract					939:945	Maytenus ilicifolia extract	919:945	Maytenus ilicifolia extract from access 116	919:961	MATERIALS AND METHODS The animals were divided into different experimental groups: normal hyperglycemic (negative control); MIA (treatment of Maytenus ilicifolia extract from access 116); MIB (treatment with Maytenus ilicifolia extract from access 122; and glipizide (positive control).
33932519	6	108	theme	different	829:837	arg1	groups					852:857	different experimental groups	829:857	different experimental groups	829:857	MATERIALS AND METHODS The animals were divided into different experimental groups: normal hyperglycemic (negative control); MIA (treatment of Maytenus ilicifolia extract from access 116); MIB (treatment with Maytenus ilicifolia extract from access 122; and glipizide (positive control).
33932519	4	109	theme	pharmacological	519:533	arg1	studies					535:541	more phytochemical and pharmacological studies	496:541	more phytochemical and pharmacological studies on the plant	496:554	However, in popular culture, this species has also been used to treat other disorders such as diabetes, but without scientific evidence, requiring more phytochemical and pharmacological studies on the plant.
33932519	13	110	theme	anti-hyperglycemic	2331:2348	arg1	effect					2350:2355	a discreet anti-hyperglycemic effect	2320:2355	a discreet anti-hyperglycemic effect	2320:2355	In addition, it was possible to conclude that the treatment with Maytenus ilicifolia had a discreet anti-hyperglycemic effect; however, it was not possible to identify the responsible mechanism, being necessary, therefore, new studies using different technologies in order to determine the possible mechanisms of action of the extract.
33932519	13	111	with	treatment	2281:2289	arg1	ilicifolia					2305:2314	Maytenus ilicifolia	2296:2314	Maytenus ilicifolia	2296:2314	In addition, it was possible to conclude that the treatment with Maytenus ilicifolia had a discreet anti-hyperglycemic effect; however, it was not possible to identify the responsible mechanism, being necessary, therefore, new studies using different technologies in order to determine the possible mechanisms of action of the extract.
33932519	6	112	with	MIA	901:903	arg1	extract					1005:1011	Maytenus ilicifolia extract	985:1011	Maytenus ilicifolia extract from access 122	985:1027	MATERIALS AND METHODS The animals were divided into different experimental groups: normal hyperglycemic (negative control); MIA (treatment of Maytenus ilicifolia extract from access 116); MIB (treatment with Maytenus ilicifolia extract from access 122; and glipizide (positive control).
33932519	11	113	from	increase	1965:1972	arg1	levels					2010:2015	triglyceride and HDL cholesterol levels	1977:2015	triglyceride and HDL cholesterol levels	1977:2015	However, hyperglycemic rats treated with the extracts showed a marked increase in triglyceride and HDL cholesterol levels.
33932519	13	114	theme	responsible	2403:2413	arg1	mechanism					2415:2423	the responsible mechanism	2399:2423	the responsible mechanism	2399:2423	In addition, it was possible to conclude that the treatment with Maytenus ilicifolia had a discreet anti-hyperglycemic effect; however, it was not possible to identify the responsible mechanism, being necessary, therefore, new studies using different technologies in order to determine the possible mechanisms of action of the extract.
33932519	9	115	theme	Maytenus	1543:1550	arg1	ilicifolia					1552:1561	Maytenus ilicifolia	1543:1561	Maytenus ilicifolia	1543:1561	RESULTS Chromatographic analysis identified oleic and palmitic acid as the most common constituents, and both extracts of Maytenus ilicifolia caused a reduction in blood glucose levels within 60 min after administration of glucose overload when compared to the normal hyperglycemic group.
33932519	6	116	with	hyperglycemic	867:879	arg1	extract					1005:1011	Maytenus ilicifolia extract	985:1011	Maytenus ilicifolia extract from access 122	985:1027	MATERIALS AND METHODS The animals were divided into different experimental groups: normal hyperglycemic (negative control); MIA (treatment of Maytenus ilicifolia extract from access 116); MIB (treatment with Maytenus ilicifolia extract from access 122; and glipizide (positive control).
34648799	8	0	theme	electron	974:981	arg1	microscopy					983:992	Scanning electron microscopy	965:992	Scanning electron microscopy	965:992	Compact surfaces of the GPE infused CH films were unveiled through Scanning electron microscopy.
34648799	0	1	theme	peel	74:77	arg1	extract					79:85	waste Garlic peel extract	61:85	waste Garlic peel extract	61:85	Development of multifunctional food packaging films based on waste Garlic peel extract and Chitosan.
34648799	4	2	from	CH	521:522	arg1	blends					539:544	the blends	535:544	the blends that improved the polymers' compatibility	535:586	Fourier transform infrared showed the interactions through hydrogen bonding between CH and GPE in the blends that improved the polymers' compatibility.
34648799	1	3	theme	waste	172:176	arg1	extract					190:196	waste garlic peel extract	172:196	waste garlic peel extract (GPE)	172:202	A sustainable multifunctional food packaging composite film containing waste garlic peel extract (GPE) and Chitosan (CH) was prepared.
34648799	1	3	theme	waste	172:176	arg1	GPE					199:201	GPE	199:201	GPE	199:201	A sustainable multifunctional food packaging composite film containing waste garlic peel extract (GPE) and Chitosan (CH) was prepared.
34648799	5	4	theme	X-ray	602:606	arg1	analysis					620:627	X-ray diffraction analysis	602:627	X-ray diffraction analysis	602:627	Furthermore, X-ray diffraction analysis validated the compatibility between GPE and CH.
34648799	4	5	dep	transform	445:453	arg1	infrared					455:462	infrared	455:462	transform infrared	445:462	Fourier transform infrared showed the interactions through hydrogen bonding between CH and GPE in the blends that improved the polymers' compatibility.
34648799	3	6	theme	physical	366:373	arg1	properties					391:400	morphological, physical, and functional properties	351:400	morphological, physical, and functional properties	351:400	GPE/CH films' morphological, physical, and functional properties were compared to those of CH film.
34648799	5	7	theme	diffraction	608:618	arg1	analysis					620:627	X-ray diffraction analysis	602:627	X-ray diffraction analysis	602:627	Furthermore, X-ray diffraction analysis validated the compatibility between GPE and CH.
34648799	8	8	theme	infused	926:932	arg1	films					937:941	the GPE infused CH films	918:941	the GPE infused CH films	918:941	Compact surfaces of the GPE infused CH films were unveiled through Scanning electron microscopy.
34648799	4	9	dep	Fourier	437:443	arg1	transform					445:453	transform	445:453	transform infrared	445:462	Fourier transform infrared showed the interactions through hydrogen bonding between CH and GPE in the blends that improved the polymers' compatibility.
34648799	1	10	contain	containing	161:170	arg2	Chitosan					208:215	Chitosan	208:215	Chitosan (CH)	208:220	A sustainable multifunctional food packaging composite film containing waste garlic peel extract (GPE) and Chitosan (CH) was prepared.
34648799	1	10	contain	containing	161:170	arg1	film					156:159	A sustainable multifunctional food packaging composite film	101:159	A sustainable multifunctional food packaging composite film containing waste garlic peel extract (GPE) and Chitosan (CH)	101:220	A sustainable multifunctional food packaging composite film containing waste garlic peel extract (GPE) and Chitosan (CH) was prepared.
34648799	1	10	contain	containing	161:170	arg2	GPE					199:201	GPE	199:201	GPE	199:201	A sustainable multifunctional food packaging composite film containing waste garlic peel extract (GPE) and Chitosan (CH) was prepared.
34648799	1	10	contain	containing	161:170	arg2	CH					218:219	CH	218:219	CH	218:219	A sustainable multifunctional food packaging composite film containing waste garlic peel extract (GPE) and Chitosan (CH) was prepared.
34648799	1	10	contain	containing	161:170	arg2	extract					190:196	waste garlic peel extract	172:196	waste garlic peel extract (GPE)	172:202	A sustainable multifunctional food packaging composite film containing waste garlic peel extract (GPE) and Chitosan (CH) was prepared.
34648799	11	11	theme	petrochemical	1362:1374	arg1	plastics					1376:1383	the commonly used petrochemical plastics	1344:1383	the commonly used petrochemical plastics	1344:1383	The results suggest that GPE/CH films can find widespread commercial applications like food packaging materials, replacing the commonly used petrochemical plastics.
34648799	2	12	theme	antimicrobial	256:268	arg1	potential					270:278	antimicrobial potential	256:278	antimicrobial potential towards Staphylococcus aureus and Klebsiella pneumoniae	256:334	This film exhibited antimicrobial potential towards Staphylococcus aureus and Klebsiella pneumoniae.
34648799	6	13	theme	moisture	721:728	arg1	content					730:736	moisture content	721:736	moisture content	721:736	GPE/CH films exhibited higher thickness and moisture content than the CH film.
34648799	1	14	theme	garlic	178:183	arg1	extract					190:196	waste garlic peel extract	172:196	waste garlic peel extract (GPE)	172:202	A sustainable multifunctional food packaging composite film containing waste garlic peel extract (GPE) and Chitosan (CH) was prepared.
34648799	1	14	theme	garlic	178:183	arg1	GPE					199:201	GPE	199:201	GPE	199:201	A sustainable multifunctional food packaging composite film containing waste garlic peel extract (GPE) and Chitosan (CH) was prepared.
34648799	3	15	theme	morphological	351:363	arg1	properties					391:400	morphological, physical, and functional properties	351:400	morphological, physical, and functional properties	351:400	GPE/CH films' morphological, physical, and functional properties were compared to those of CH film.
34648799	4	16	theme	hydrogen	496:503	arg1	bonding					505:511	hydrogen bonding	496:511	hydrogen bonding	496:511	Fourier transform infrared showed the interactions through hydrogen bonding between CH and GPE in the blends that improved the polymers' compatibility.
34648799	9	17	theme	improved	1014:1021	arg1	stability					1031:1039	improved thermal stability	1014:1039	improved thermal stability	1014:1039	GPE/CH film showed improved thermal stability after the addition of GPE.
34648799	7	18	theme	mechanical	864:873	arg1	strength					875:882	mechanical strength	864:882	mechanical strength	864:882	Remarkably, GPE/CH films showed lower water vapor barrier properties and higher ultra-violet protection and mechanical strength than CH film.
34648799	1	19	theme	peel	185:188	arg1	extract					190:196	waste garlic peel extract	172:196	waste garlic peel extract (GPE)	172:202	A sustainable multifunctional food packaging composite film containing waste garlic peel extract (GPE) and Chitosan (CH) was prepared.
34648799	1	19	theme	peel	185:188	arg1	GPE					199:201	GPE	199:201	GPE	199:201	A sustainable multifunctional food packaging composite film containing waste garlic peel extract (GPE) and Chitosan (CH) was prepared.
34648799	3	20	theme	functional	380:389	arg1	properties					391:400	morphological, physical, and functional properties	351:400	morphological, physical, and functional properties	351:400	GPE/CH films' morphological, physical, and functional properties were compared to those of CH film.
34648799	0	21	theme	Garlic	67:72	arg1	extract					79:85	waste Garlic peel extract	61:85	waste Garlic peel extract	61:85	Development of multifunctional food packaging films based on waste Garlic peel extract and Chitosan.
34648799	8	22	theme	GPE	922:924	arg1	films					937:941	the GPE infused CH films	918:941	the GPE infused CH films	918:941	Compact surfaces of the GPE infused CH films were unveiled through Scanning electron microscopy.
34648799	8	23	theme	Compact	898:904	arg1	surfaces					906:913	Compact surfaces	898:913	Compact surfaces of the GPE infused CH films	898:941	Compact surfaces of the GPE infused CH films were unveiled through Scanning electron microscopy.
34648799	9	24	theme	GPE/CH	995:1000	arg1	film					1002:1005	GPE/CH film	995:1005	GPE/CH film	995:1005	GPE/CH film showed improved thermal stability after the addition of GPE.
34648799	11	25	theme	used	1357:1360	arg1	plastics					1376:1383	the commonly used petrochemical plastics	1344:1383	the commonly used petrochemical plastics	1344:1383	The results suggest that GPE/CH films can find widespread commercial applications like food packaging materials, replacing the commonly used petrochemical plastics.
34648799	10	26	dep	study	1094:1098	arg1	manifested					1100:1109	manifested	1100:1109	study manifested that the GPE/CH films are antioxidant and non-cytotoxic, implicating their biocompatibility and non-toxicity	1094:1218	MTT method's cytotoxicity study manifested that the GPE/CH films are antioxidant and non-cytotoxic, implicating their biocompatibility and non-toxicity.
34648799	6	27	theme	higher	700:705	arg1	thickness					707:715	higher thickness	700:715	higher thickness	700:715	GPE/CH films exhibited higher thickness and moisture content than the CH film.
34648799	0	28	theme	food	31:34	arg1	films					46:50	multifunctional food packaging films	15:50	multifunctional food packaging films	15:50	Development of multifunctional food packaging films based on waste Garlic peel extract and Chitosan.
34648799	11	29	theme	widespread	1268:1277	arg1	applications					1290:1301	widespread commercial applications	1268:1301	widespread commercial applications	1268:1301	The results suggest that GPE/CH films can find widespread commercial applications like food packaging materials, replacing the commonly used petrochemical plastics.
34648799	0	30	theme	multifunctional	15:29	arg1	films					46:50	multifunctional food packaging films	15:50	multifunctional food packaging films	15:50	Development of multifunctional food packaging films based on waste Garlic peel extract and Chitosan.
34648799	7	31	theme	vapor	800:804	arg1	barrier					806:812	lower water vapor barrier	788:812	lower water vapor barrier properties	788:823	Remarkably, GPE/CH films showed lower water vapor barrier properties and higher ultra-violet protection and mechanical strength than CH film.
34648799	11	32	theme	commercial	1279:1288	arg1	applications					1290:1301	widespread commercial applications	1268:1301	widespread commercial applications	1268:1301	The results suggest that GPE/CH films can find widespread commercial applications like food packaging materials, replacing the commonly used petrochemical plastics.
34648799	7	33	theme	ultra-violet	836:847	arg1	protection					849:858	higher ultra-violet protection	829:858	higher ultra-violet protection	829:858	Remarkably, GPE/CH films showed lower water vapor barrier properties and higher ultra-violet protection and mechanical strength than CH film.
34648799	7	34	theme	higher	829:834	arg1	protection					849:858	higher ultra-violet protection	829:858	higher ultra-violet protection	829:858	Remarkably, GPE/CH films showed lower water vapor barrier properties and higher ultra-violet protection and mechanical strength than CH film.
34648799	0	35	theme	films	46:50	arg1	Development					0:10	Development	0:10	Development of multifunctional food packaging films	0:50	Development of multifunctional food packaging films based on waste Garlic peel extract and Chitosan.
34648799	7	36	theme	barrier	806:812	arg1	properties					814:823	lower water vapor barrier properties	788:823	lower water vapor barrier properties	788:823	Remarkably, GPE/CH films showed lower water vapor barrier properties and higher ultra-violet protection and mechanical strength than CH film.
34648799	6	37	theme	GPE/CH	677:682	arg1	films					684:688	GPE/CH films	677:688	GPE/CH films	677:688	GPE/CH films exhibited higher thickness and moisture content than the CH film.
34648799	0	38	theme	packaging	36:44	arg1	films					46:50	multifunctional food packaging films	15:50	multifunctional food packaging films	15:50	Development of multifunctional food packaging films based on waste Garlic peel extract and Chitosan.
34648799	7	39	theme	lower	788:792	arg1	barrier					806:812	lower water vapor barrier	788:812	lower water vapor barrier properties	788:823	Remarkably, GPE/CH films showed lower water vapor barrier properties and higher ultra-violet protection and mechanical strength than CH film.
34648799	9	40	theme	thermal	1023:1029	arg1	stability					1031:1039	improved thermal stability	1014:1039	improved thermal stability	1014:1039	GPE/CH film showed improved thermal stability after the addition of GPE.
34648799	8	41	theme	films	937:941	arg1	surfaces					906:913	Compact surfaces	898:913	Compact surfaces of the GPE infused CH films	898:941	Compact surfaces of the GPE infused CH films were unveiled through Scanning electron microscopy.
34648799	7	42	theme	water	794:798	arg1	barrier					806:812	lower water vapor barrier	788:812	lower water vapor barrier properties	788:823	Remarkably, GPE/CH films showed lower water vapor barrier properties and higher ultra-violet protection and mechanical strength than CH film.
34648799	11	43	theme	food	1308:1311	arg1	materials					1323:1331	food packaging materials	1308:1331	food packaging materials	1308:1331	The results suggest that GPE/CH films can find widespread commercial applications like food packaging materials, replacing the commonly used petrochemical plastics.
34648799	10	44	theme	GPE/CH	1120:1125	arg1	antioxidant					1137:1147	antioxidant	1137:1147	antioxidant	1137:1147	MTT method's cytotoxicity study manifested that the GPE/CH films are antioxidant and non-cytotoxic, implicating their biocompatibility and non-toxicity.
34648799	10	44	theme	GPE/CH	1120:1125	arg1	films					1127:1131	the GPE/CH films	1116:1131	the GPE/CH films	1116:1131	MTT method's cytotoxicity study manifested that the GPE/CH films are antioxidant and non-cytotoxic, implicating their biocompatibility and non-toxicity.
34648799	8	45	theme	CH	934:935	arg1	films					937:941	the GPE infused CH films	918:941	the GPE infused CH films	918:941	Compact surfaces of the GPE infused CH films were unveiled through Scanning electron microscopy.
34648799	1	46	theme	sustainable	103:113	arg1	film					156:159	A sustainable multifunctional food packaging composite film	101:159	A sustainable multifunctional food packaging composite film containing waste garlic peel extract (GPE) and Chitosan (CH)	101:220	A sustainable multifunctional food packaging composite film containing waste garlic peel extract (GPE) and Chitosan (CH) was prepared.
34648799	4	47	from	GPE	528:530	arg1	blends					539:544	the blends	535:544	the blends that improved the polymers' compatibility	535:586	Fourier transform infrared showed the interactions through hydrogen bonding between CH and GPE in the blends that improved the polymers' compatibility.
34648799	11	48	theme	packaging	1313:1321	arg1	materials					1323:1331	food packaging materials	1308:1331	food packaging materials	1308:1331	The results suggest that GPE/CH films can find widespread commercial applications like food packaging materials, replacing the commonly used petrochemical plastics.
34648799	11	49	theme	GPE/CH	1246:1251	arg1	films					1253:1257	GPE/CH films	1246:1257	GPE/CH films	1246:1257	The results suggest that GPE/CH films can find widespread commercial applications like food packaging materials, replacing the commonly used petrochemical plastics.
34648799	1	50	theme	multifunctional	115:129	arg1	film					156:159	A sustainable multifunctional food packaging composite film	101:159	A sustainable multifunctional food packaging composite film containing waste garlic peel extract (GPE) and Chitosan (CH)	101:220	A sustainable multifunctional food packaging composite film containing waste garlic peel extract (GPE) and Chitosan (CH) was prepared.
34648799	1	51	theme	food	131:134	arg1	film					156:159	A sustainable multifunctional food packaging composite film	101:159	A sustainable multifunctional food packaging composite film containing waste garlic peel extract (GPE) and Chitosan (CH)	101:220	A sustainable multifunctional food packaging composite film containing waste garlic peel extract (GPE) and Chitosan (CH) was prepared.
34648799	10	52	theme	MTT	1068:1070	arg1	method					1072:1077	MTT method's	1068:1079	MTT method's cytotoxicity	1068:1092	MTT method's cytotoxicity study manifested that the GPE/CH films are antioxidant and non-cytotoxic, implicating their biocompatibility and non-toxicity.
34648799	7	53	theme	CH	889:890	arg1	film					892:895	CH film	889:895	CH film	889:895	Remarkably, GPE/CH films showed lower water vapor barrier properties and higher ultra-violet protection and mechanical strength than CH film.
34648799	8	54	theme	Scanning	965:972	arg1	microscopy					983:992	Scanning electron microscopy	965:992	Scanning electron microscopy	965:992	Compact surfaces of the GPE infused CH films were unveiled through Scanning electron microscopy.
34648799	1	55	theme	packaging	136:144	arg1	film					156:159	A sustainable multifunctional food packaging composite film	101:159	A sustainable multifunctional food packaging composite film containing waste garlic peel extract (GPE) and Chitosan (CH)	101:220	A sustainable multifunctional food packaging composite film containing waste garlic peel extract (GPE) and Chitosan (CH) was prepared.
34648799	7	56	theme	GPE/CH	768:773	arg1	films					775:779	GPE/CH films	768:779	GPE/CH films	768:779	Remarkably, GPE/CH films showed lower water vapor barrier properties and higher ultra-violet protection and mechanical strength than CH film.
34648799	3	57	theme	GPE/CH	337:342	arg1	films					344:348	GPE/CH films	337:348	GPE/CH films	337:348	GPE/CH films' morphological, physical, and functional properties were compared to those of CH film.
34648799	6	58	theme	CH	747:748	arg1	film					750:753	the CH film	743:753	the CH film	743:753	GPE/CH films exhibited higher thickness and moisture content than the CH film.
34648799	1	59	theme	composite	146:154	arg1	film					156:159	A sustainable multifunctional food packaging composite film	101:159	A sustainable multifunctional food packaging composite film containing waste garlic peel extract (GPE) and Chitosan (CH)	101:220	A sustainable multifunctional food packaging composite film containing waste garlic peel extract (GPE) and Chitosan (CH) was prepared.
34648799	9	60	theme	GPE	1063:1065	arg1	addition					1051:1058	the addition	1047:1058	the addition of GPE	1047:1065	GPE/CH film showed improved thermal stability after the addition of GPE.
34648799	0	61	theme	waste	61:65	arg1	extract					79:85	waste Garlic peel extract	61:85	waste Garlic peel extract	61:85	Development of multifunctional food packaging films based on waste Garlic peel extract and Chitosan.
34576227	2	0	theme	complete	578:585	arg1	loss					587:590	complete loss	578:590	complete loss of the limbal niche tissue structure	578:627	A scaffold with niche-specific structure and extracellular matrix (ECM) composition might have the advantage to improve long-term clinical outcomes, in particular for patients with severe damage or complete loss of the limbal niche tissue structure.
34576227	6	1	theme	cultured	1077:1084	arg1	LEPCs					1086:1090	cultured LEPCs	1077:1090	cultured LEPCs	1077:1090	The recellularization of these scaffolds was studied by plating cultured LEPCs and limbal melanocytes (LMs) or by allowing cells to migrate from the host tissue following a lamellar transplantation ex vivo.
34576227	4	2	theme	deoxyribonuclease	818:834	arg1	I					836:836	deoxyribonuclease I	818:836	deoxyribonuclease I	818:836	Corneoscleral tissue was decellularized by sodium deoxycholate and deoxyribonuclease I in the presence or absence of dextran.
34576227	1	3	theme	damaged	257:263	arg1	surface					273:279	the damaged corneal surface	253:279	the damaged corneal surface	253:279	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	2	4	theme	limbal	599:604	arg1	structure					619:627	the limbal niche tissue structure	595:627	the limbal niche tissue structure	595:627	A scaffold with niche-specific structure and extracellular matrix (ECM) composition might have the advantage to improve long-term clinical outcomes, in particular for patients with severe damage or complete loss of the limbal niche tissue structure.
34576227	2	5	with	scaffold	382:389	arg1	composition					452:462	extracellular matrix (ECM) composition	425:462	extracellular matrix (ECM) composition	425:462	A scaffold with niche-specific structure and extracellular matrix (ECM) composition might have the advantage to improve long-term clinical outcomes, in particular for patients with severe damage or complete loss of the limbal niche tissue structure.
34576227	2	5	with	scaffold	382:389	arg1	structure					411:419	niche-specific structure	396:419	niche-specific structure	396:419	A scaffold with niche-specific structure and extracellular matrix (ECM) composition might have the advantage to improve long-term clinical outcomes, in particular for patients with severe damage or complete loss of the limbal niche tissue structure.
34576227	1	6	with	patients	284:291	arg1	LSCD					327:330	LSCD	327:330	LSCD	327:330	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	1	6	with	patients	284:291	arg1	deficiency					315:324	limbal stem cell deficiency	298:324	limbal stem cell deficiency (LSCD)	298:331	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	8	7	dep	In	1358:1359	arg1	vitro					1361:1365	vitro	1361:1365	vitro	1361:1365	In vitro recellularization by LEPCs and LMs demonstrated the good biocompatibility of the DHL and intrastromal invasion of LEPCs.
34576227	1	8	theme	success	352:358	arg1	restricted					368:377	restricted	368:377	restricted	368:377	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	1	8	theme	success	352:358	arg1	rate					360:363	the long-term success rate	338:363	the long-term success rate	338:363	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	1	9	theme	fibrin	189:194	arg1	gel					196:198	amniotic membrane or fibrin gel	168:198	gel	196:198	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	6	10	theme	ex	1211:1212	arg1	transplantation					1195:1209	a lamellar transplantation	1184:1209	a lamellar transplantation ex vivo	1184:1217	The recellularization of these scaffolds was studied by plating cultured LEPCs and limbal melanocytes (LMs) or by allowing cells to migrate from the host tissue following a lamellar transplantation ex vivo.
34576227	4	11	theme	dextran	868:874	arg1	presence					845:852	presence	845:852	presence	845:852	Corneoscleral tissue was decellularized by sodium deoxycholate and deoxyribonuclease I in the presence or absence of dextran.
34576227	4	11	theme	dextran	868:874	arg1	absence					857:863	absence	857:863	absence	857:863	Corneoscleral tissue was decellularized by sodium deoxycholate and deoxyribonuclease I in the presence or absence of dextran.
34576227	6	12	theme	lamellar	1186:1193	arg1	transplantation					1195:1209	a lamellar transplantation	1184:1209	a lamellar transplantation ex vivo	1184:1217	The recellularization of these scaffolds was studied by plating cultured LEPCs and limbal melanocytes (LMs) or by allowing cells to migrate from the host tissue following a lamellar transplantation ex vivo.
34576227	5	13	dep	ultrastructure	949:962	arg1	the					945:947	the	945:947	the	945:947	We evaluated the efficiency of decellularization and its effects on the ultrastructure and ECM composition of the human corneal limbus.
34576227	1	14	theme	expanded	113:120	arg1	cells					151:155	limbal epithelial progenitor cells	122:155	ex vivo expanded limbal epithelial progenitor cells (LEPCs)	105:163	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	1	14	theme	expanded	113:120	arg1	LEPCs					158:162	LEPCs	158:162	LEPCs	158:162	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	8	15	theme	invasion	1469:1476	arg1	biocompatibility					1424:1439	the good biocompatibility	1415:1439	the good biocompatibility of the DHL and intrastromal invasion of LEPCs	1415:1485	In vitro recellularization by LEPCs and LMs demonstrated the good biocompatibility of the DHL and intrastromal invasion of LEPCs.
34576227	1	16	theme	epithelial	129:138	arg1	cells					151:155	limbal epithelial progenitor cells	122:155	ex vivo expanded limbal epithelial progenitor cells (LEPCs)	105:163	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	1	16	theme	epithelial	129:138	arg1	LEPCs					158:162	LEPCs	158:162	LEPCs	158:162	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	8	17	theme	LEPCs	1481:1485	arg1	DHL					1448:1450	DHL	1448:1450	DHL	1448:1450	In vitro recellularization by LEPCs and LMs demonstrated the good biocompatibility of the DHL and intrastromal invasion of LEPCs.
34576227	8	17	theme	LEPCs	1481:1485	arg1	invasion					1469:1476	intrastromal invasion	1456:1476	intrastromal invasion	1456:1476	In vitro recellularization by LEPCs and LMs demonstrated the good biocompatibility of the DHL and intrastromal invasion of LEPCs.
34576227	7	18	theme	native	1334:1339	arg1	composition					1345:1355	the native ECM composition	1330:1355	the native ECM composition	1330:1355	Our decellularization protocol rapidly and effectively removed cellular and nuclear material while preserving the native ECM composition.
34576227	0	19	theme	Cell	55:58	arg1	Reconstruction					66:79	Limbal Stem Cell Niche Reconstruction	43:79	Limbal Stem Cell Niche Reconstruction	43:79	A Decellularized Human Limbal Scaffold for Limbal Stem Cell Niche Reconstruction.
34576227	1	20	theme	cells	151:155	arg1	transplantation					86:100	The transplantation	82:100	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel	82:198	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	1	20	theme	cells	151:155	arg1	strategy					230:237	an established therapeutic strategy	203:237	an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD)	203:331	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	6	21	dep	ex	1211:1212	arg1	vivo					1214:1217	vivo	1214:1217	vivo	1214:1217	The recellularization of these scaffolds was studied by plating cultured LEPCs and limbal melanocytes (LMs) or by allowing cells to migrate from the host tissue following a lamellar transplantation ex vivo.
34576227	2	22	theme	long-term	500:508	arg1	outcomes					519:526	long-term clinical outcomes	500:526	long-term clinical outcomes	500:526	A scaffold with niche-specific structure and extracellular matrix (ECM) composition might have the advantage to improve long-term clinical outcomes, in particular for patients with severe damage or complete loss of the limbal niche tissue structure.
34576227	2	23	theme	structure	619:627	arg1	damage					568:573	severe damage	561:573	severe damage	561:573	A scaffold with niche-specific structure and extracellular matrix (ECM) composition might have the advantage to improve long-term clinical outcomes, in particular for patients with severe damage or complete loss of the limbal niche tissue structure.
34576227	2	23	theme	structure	619:627	arg1	loss					587:590	complete loss	578:590	complete loss of the limbal niche tissue structure	578:627	A scaffold with niche-specific structure and extracellular matrix (ECM) composition might have the advantage to improve long-term clinical outcomes, in particular for patients with severe damage or complete loss of the limbal niche tissue structure.
34576227	2	24	theme	niche	606:610	arg1	structure					619:627	the limbal niche tissue structure	595:627	the limbal niche tissue structure	595:627	A scaffold with niche-specific structure and extracellular matrix (ECM) composition might have the advantage to improve long-term clinical outcomes, in particular for patients with severe damage or complete loss of the limbal niche tissue structure.
34576227	9	25	dep	Ex	1488:1489	arg1	vivo					1491:1494	vivo	1491:1494	vivo	1491:1494	Ex vivo transplantation of DHL revealed complete epithelialization as well as melanocytic and stromal repopulation from the host tissue.
34576227	5	26	from	efficiency	894:903	arg1	ultrastructure					949:962	ultrastructure	949:962	ultrastructure	949:962	We evaluated the efficiency of decellularization and its effects on the ultrastructure and ECM composition of the human corneal limbus.
34576227	5	26	from	efficiency	894:903	arg1	composition					972:982	ECM composition	968:982	ECM composition	968:982	We evaluated the efficiency of decellularization and its effects on the ultrastructure and ECM composition of the human corneal limbus.
34576227	9	27	theme	melanocytic	1566:1576	arg1	repopulation					1590:1601	melanocytic and stromal repopulation	1566:1601	complete epithelialization as well as melanocytic and stromal repopulation from the host tissue	1528:1622	Ex vivo transplantation of DHL revealed complete epithelialization as well as melanocytic and stromal repopulation from the host tissue.
34576227	2	28	theme	extracellular	425:437	arg1	ECM					447:449	ECM	447:449	ECM	447:449	A scaffold with niche-specific structure and extracellular matrix (ECM) composition might have the advantage to improve long-term clinical outcomes, in particular for patients with severe damage or complete loss of the limbal niche tissue structure.
34576227	2	28	theme	extracellular	425:437	arg1	matrix					439:444	extracellular matrix	425:444	extracellular matrix (ECM) composition	425:462	A scaffold with niche-specific structure and extracellular matrix (ECM) composition might have the advantage to improve long-term clinical outcomes, in particular for patients with severe damage or complete loss of the limbal niche tissue structure.
34576227	1	29	theme	corneal	265:271	arg1	surface					273:279	the damaged corneal surface	253:279	the damaged corneal surface	253:279	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	9	30	theme	stromal	1582:1588	arg1	repopulation					1590:1601	melanocytic and stromal repopulation	1566:1601	complete epithelialization as well as melanocytic and stromal repopulation from the host tissue	1528:1622	Ex vivo transplantation of DHL revealed complete epithelialization as well as melanocytic and stromal repopulation from the host tissue.
34576227	9	31	from	tissue	1617:1622	arg1	repopulation					1590:1601	melanocytic and stromal repopulation	1566:1601	complete epithelialization as well as melanocytic and stromal repopulation from the host tissue	1528:1622	Ex vivo transplantation of DHL revealed complete epithelialization as well as melanocytic and stromal repopulation from the host tissue.
34576227	9	31	from	tissue	1617:1622	arg1	epithelialization					1537:1553	complete epithelialization	1528:1553	complete epithelialization as well as melanocytic and stromal repopulation from the host tissue	1528:1622	Ex vivo transplantation of DHL revealed complete epithelialization as well as melanocytic and stromal repopulation from the host tissue.
34576227	6	32	theme	scaffolds	1044:1052	arg1	recellularization					1017:1033	The recellularization	1013:1033	The recellularization of these scaffolds	1013:1052	The recellularization of these scaffolds was studied by plating cultured LEPCs and limbal melanocytes (LMs) or by allowing cells to migrate from the host tissue following a lamellar transplantation ex vivo.
34576227	6	33	theme	host	1162:1165	arg1	tissue					1167:1172	the host tissue	1158:1172	the host tissue	1158:1172	The recellularization of these scaffolds was studied by plating cultured LEPCs and limbal melanocytes (LMs) or by allowing cells to migrate from the host tissue following a lamellar transplantation ex vivo.
34576227	0	34	theme	Human	17:21	arg1	Scaffold					30:37	A Decellularized Human Limbal Scaffold	0:37	A Decellularized Human Limbal Scaffold for Limbal Stem Cell Niche Reconstruction.	0:80	A Decellularized Human Limbal Scaffold for Limbal Stem Cell Niche Reconstruction.
34576227	7	35	theme	nuclear	1296:1302	arg1	material					1304:1311	cellular and nuclear material	1283:1311	cellular and nuclear material	1283:1311	Our decellularization protocol rapidly and effectively removed cellular and nuclear material while preserving the native ECM composition.
34576227	10	36	theme	promising	1669:1677	arg1	scaffold					1649:1656	the generated DHL scaffold	1631:1656	the generated DHL scaffold	1631:1656	Thus, the generated DHL scaffold could be a promising biological material as a carrier for the transplantation of LEPCs to treat LSCD.
34576227	10	36	theme	promising	1669:1677	arg1	material					1690:1697	a promising biological material	1667:1697	a promising biological material	1667:1697	Thus, the generated DHL scaffold could be a promising biological material as a carrier for the transplantation of LEPCs to treat LSCD.
34576227	9	37	theme	host	1612:1615	arg1	tissue					1617:1622	the host tissue	1608:1622	the host tissue	1608:1622	Ex vivo transplantation of DHL revealed complete epithelialization as well as melanocytic and stromal repopulation from the host tissue.
34576227	7	38	theme	cellular	1283:1290	arg1	material					1304:1311	cellular and nuclear material	1283:1311	cellular and nuclear material	1283:1311	Our decellularization protocol rapidly and effectively removed cellular and nuclear material while preserving the native ECM composition.
34576227	0	39	theme	Limbal	43:48	arg1	Reconstruction					66:79	Limbal Stem Cell Niche Reconstruction	43:79	Limbal Stem Cell Niche Reconstruction	43:79	A Decellularized Human Limbal Scaffold for Limbal Stem Cell Niche Reconstruction.
34576227	8	40	dep	DHL	1448:1450	arg1	the					1444:1446	the	1444:1446	the	1444:1446	In vitro recellularization by LEPCs and LMs demonstrated the good biocompatibility of the DHL and intrastromal invasion of LEPCs.
34576227	8	41	theme	good	1419:1422	arg1	biocompatibility					1424:1439	the good biocompatibility	1415:1439	the good biocompatibility of the DHL and intrastromal invasion of LEPCs	1415:1485	In vitro recellularization by LEPCs and LMs demonstrated the good biocompatibility of the DHL and intrastromal invasion of LEPCs.
34576227	5	42	theme	corneal	997:1003	arg1	limbus					1005:1010	the human corneal limbus	987:1010	the human corneal limbus	987:1010	We evaluated the efficiency of decellularization and its effects on the ultrastructure and ECM composition of the human corneal limbus.
34576227	5	43	from	effects	934:940	arg1	ultrastructure					949:962	ultrastructure	949:962	ultrastructure	949:962	We evaluated the efficiency of decellularization and its effects on the ultrastructure and ECM composition of the human corneal limbus.
34576227	5	43	from	effects	934:940	arg1	composition					972:982	ECM composition	968:982	ECM composition	968:982	We evaluated the efficiency of decellularization and its effects on the ultrastructure and ECM composition of the human corneal limbus.
34576227	6	44	theme	limbal	1096:1101	arg1	melanocytes					1103:1113	limbal melanocytes	1096:1113	limbal melanocytes (LMs)	1096:1119	The recellularization of these scaffolds was studied by plating cultured LEPCs and limbal melanocytes (LMs) or by allowing cells to migrate from the host tissue following a lamellar transplantation ex vivo.
34576227	6	44	theme	limbal	1096:1101	arg1	LMs					1116:1118	LMs	1116:1118	LMs	1116:1118	The recellularization of these scaffolds was studied by plating cultured LEPCs and limbal melanocytes (LMs) or by allowing cells to migrate from the host tissue following a lamellar transplantation ex vivo.
34576227	10	45	theme	LEPCs	1739:1743	arg1	transplantation					1720:1734	the transplantation	1716:1734	the transplantation of LEPCs to treat LSCD	1716:1757	Thus, the generated DHL scaffold could be a promising biological material as a carrier for the transplantation of LEPCs to treat LSCD.
34576227	1	46	from	transplantation	86:100	arg1	membrane					177:184	amniotic membrane or fibrin gel	168:198	membrane	177:184	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	1	46	from	transplantation	86:100	arg1	gel					196:198	amniotic membrane or fibrin gel	168:198	gel	196:198	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	1	47	theme	therapeutic	218:228	arg1	transplantation					86:100	The transplantation	82:100	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel	82:198	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	1	47	theme	therapeutic	218:228	arg1	strategy					230:237	an established therapeutic strategy	203:237	an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD)	203:331	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	4	48	dep	presence	845:852	arg1	the					841:843	the	841:843	the	841:843	Corneoscleral tissue was decellularized by sodium deoxycholate and deoxyribonuclease I in the presence or absence of dextran.
34576227	7	49	theme	decellularization	1224:1240	arg1	protocol					1242:1249	Our decellularization protocol	1220:1249	Our decellularization protocol	1220:1249	Our decellularization protocol rapidly and effectively removed cellular and nuclear material while preserving the native ECM composition.
34576227	3	50	theme	decellularized	658:671	arg1	scaffold					708:715	a biomimetic scaffold	695:715	a biomimetic scaffold for the transplantation of LEPCs	695:748	Therefore, we evaluated the decellularized human limbus (DHL) as a biomimetic scaffold for the transplantation of LEPCs.
34576227	3	50	theme	decellularized	658:671	arg1	DHL					687:689	DHL	687:689	DHL	687:689	Therefore, we evaluated the decellularized human limbus (DHL) as a biomimetic scaffold for the transplantation of LEPCs.
34576227	3	50	theme	decellularized	658:671	arg1	limbus					679:684	the decellularized human limbus	654:684	the decellularized human limbus (DHL)	654:690	Therefore, we evaluated the decellularized human limbus (DHL) as a biomimetic scaffold for the transplantation of LEPCs.
34576227	2	51	with	patients	547:554	arg1	damage					568:573	severe damage	561:573	severe damage	561:573	A scaffold with niche-specific structure and extracellular matrix (ECM) composition might have the advantage to improve long-term clinical outcomes, in particular for patients with severe damage or complete loss of the limbal niche tissue structure.
34576227	2	51	with	patients	547:554	arg1	loss					587:590	complete loss	578:590	complete loss of the limbal niche tissue structure	578:627	A scaffold with niche-specific structure and extracellular matrix (ECM) composition might have the advantage to improve long-term clinical outcomes, in particular for patients with severe damage or complete loss of the limbal niche tissue structure.
34576227	2	52	theme	severe	561:566	arg1	damage					568:573	severe damage	561:573	severe damage	561:573	A scaffold with niche-specific structure and extracellular matrix (ECM) composition might have the advantage to improve long-term clinical outcomes, in particular for patients with severe damage or complete loss of the limbal niche tissue structure.
34576227	1	53	theme	stem	305:308	arg1	cell					310:313	limbal stem cell	298:313	limbal stem cell deficiency (LSCD)	298:331	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	1	54	theme	amniotic	168:175	arg1	membrane					177:184	amniotic membrane or fibrin gel	168:198	membrane	177:184	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	8	55	theme	DHL	1448:1450	arg1	biocompatibility					1424:1439	the good biocompatibility	1415:1439	the good biocompatibility of the DHL and intrastromal invasion of LEPCs	1415:1485	In vitro recellularization by LEPCs and LMs demonstrated the good biocompatibility of the DHL and intrastromal invasion of LEPCs.
34576227	1	56	theme	progenitor	140:149	arg1	cells					151:155	limbal epithelial progenitor cells	122:155	ex vivo expanded limbal epithelial progenitor cells (LEPCs)	105:163	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	1	56	theme	progenitor	140:149	arg1	LEPCs					158:162	LEPCs	158:162	LEPCs	158:162	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	10	57	theme	generated	1635:1643	arg1	scaffold					1649:1656	the generated DHL scaffold	1631:1656	the generated DHL scaffold	1631:1656	Thus, the generated DHL scaffold could be a promising biological material as a carrier for the transplantation of LEPCs to treat LSCD.
34576227	10	57	theme	generated	1635:1643	arg1	material					1690:1697	a promising biological material	1667:1697	a promising biological material	1667:1697	Thus, the generated DHL scaffold could be a promising biological material as a carrier for the transplantation of LEPCs to treat LSCD.
34576227	3	58	theme	biomimetic	697:706	arg1	scaffold					708:715	a biomimetic scaffold	695:715	a biomimetic scaffold for the transplantation of LEPCs	695:748	Therefore, we evaluated the decellularized human limbus (DHL) as a biomimetic scaffold for the transplantation of LEPCs.
34576227	3	58	theme	biomimetic	697:706	arg1	limbus					679:684	the decellularized human limbus	654:684	the decellularized human limbus (DHL)	654:690	Therefore, we evaluated the decellularized human limbus (DHL) as a biomimetic scaffold for the transplantation of LEPCs.
34576227	2	59	theme	clinical	510:517	arg1	outcomes					519:526	long-term clinical outcomes	500:526	long-term clinical outcomes	500:526	A scaffold with niche-specific structure and extracellular matrix (ECM) composition might have the advantage to improve long-term clinical outcomes, in particular for patients with severe damage or complete loss of the limbal niche tissue structure.
34576227	8	60	theme	intrastromal	1456:1467	arg1	invasion					1469:1476	intrastromal invasion	1456:1476	intrastromal invasion	1456:1476	In vitro recellularization by LEPCs and LMs demonstrated the good biocompatibility of the DHL and intrastromal invasion of LEPCs.
34576227	3	61	theme	LEPCs	744:748	arg1	transplantation					725:739	the transplantation	721:739	the transplantation of LEPCs	721:748	Therefore, we evaluated the decellularized human limbus (DHL) as a biomimetic scaffold for the transplantation of LEPCs.
34576227	9	62	theme	Ex	1488:1489	arg1	transplantation					1496:1510	Ex vivo transplantation	1488:1510	Ex vivo transplantation of DHL	1488:1517	Ex vivo transplantation of DHL revealed complete epithelialization as well as melanocytic and stromal repopulation from the host tissue.
34576227	4	63	theme	Corneoscleral	751:763	arg1	tissue					765:770	Corneoscleral tissue	751:770	Corneoscleral tissue	751:770	Corneoscleral tissue was decellularized by sodium deoxycholate and deoxyribonuclease I in the presence or absence of dextran.
34576227	1	64	theme	limbal	122:127	arg1	cells					151:155	limbal epithelial progenitor cells	122:155	ex vivo expanded limbal epithelial progenitor cells (LEPCs)	105:163	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	1	64	theme	limbal	122:127	arg1	LEPCs					158:162	LEPCs	158:162	LEPCs	158:162	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	0	65	theme	Niche	60:64	arg1	Reconstruction					66:79	Limbal Stem Cell Niche Reconstruction	43:79	Limbal Stem Cell Niche Reconstruction	43:79	A Decellularized Human Limbal Scaffold for Limbal Stem Cell Niche Reconstruction.
34576227	9	66	theme	DHL	1515:1517	arg1	transplantation					1496:1510	Ex vivo transplantation	1488:1510	Ex vivo transplantation of DHL	1488:1517	Ex vivo transplantation of DHL revealed complete epithelialization as well as melanocytic and stromal repopulation from the host tissue.
34576227	7	67	theme	ECM	1341:1343	arg1	composition					1345:1355	the native ECM composition	1330:1355	the native ECM composition	1330:1355	Our decellularization protocol rapidly and effectively removed cellular and nuclear material while preserving the native ECM composition.
34576227	9	68	theme	complete	1528:1535	arg1	epithelialization					1537:1553	complete epithelialization	1528:1553	complete epithelialization as well as melanocytic and stromal repopulation from the host tissue	1528:1622	Ex vivo transplantation of DHL revealed complete epithelialization as well as melanocytic and stromal repopulation from the host tissue.
34576227	2	69	theme	tissue	612:617	arg1	structure					619:627	the limbal niche tissue structure	595:627	the limbal niche tissue structure	595:627	A scaffold with niche-specific structure and extracellular matrix (ECM) composition might have the advantage to improve long-term clinical outcomes, in particular for patients with severe damage or complete loss of the limbal niche tissue structure.
34576227	0	70	theme	Decellularized	2:15	arg1	Scaffold					30:37	A Decellularized Human Limbal Scaffold	0:37	A Decellularized Human Limbal Scaffold for Limbal Stem Cell Niche Reconstruction.	0:80	A Decellularized Human Limbal Scaffold for Limbal Stem Cell Niche Reconstruction.
34576227	1	71	theme	long-term	342:350	arg1	restricted					368:377	restricted	368:377	restricted	368:377	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	1	71	theme	long-term	342:350	arg1	rate					360:363	the long-term success rate	338:363	the long-term success rate	338:363	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	5	72	theme	decellularization	908:924	arg1	effects					934:940	its effects	930:940	its effects on the ultrastructure and ECM composition of the human corneal limbus	930:1010	We evaluated the efficiency of decellularization and its effects on the ultrastructure and ECM composition of the human corneal limbus.
34576227	5	72	theme	decellularization	908:924	arg1	efficiency					894:903	the efficiency	890:903	the efficiency of decellularization	890:924	We evaluated the efficiency of decellularization and its effects on the ultrastructure and ECM composition of the human corneal limbus.
34576227	8	73	theme	In	1358:1359	arg1	recellularization					1367:1383	In vitro recellularization	1358:1383	In vitro recellularization by LEPCs and LMs	1358:1400	In vitro recellularization by LEPCs and LMs demonstrated the good biocompatibility of the DHL and intrastromal invasion of LEPCs.
34576227	10	74	theme	DHL	1645:1647	arg1	scaffold					1649:1656	the generated DHL scaffold	1631:1656	the generated DHL scaffold	1631:1656	Thus, the generated DHL scaffold could be a promising biological material as a carrier for the transplantation of LEPCs to treat LSCD.
34576227	10	74	theme	DHL	1645:1647	arg1	material					1690:1697	a promising biological material	1667:1697	a promising biological material	1667:1697	Thus, the generated DHL scaffold could be a promising biological material as a carrier for the transplantation of LEPCs to treat LSCD.
34576227	0	75	theme	Limbal	23:28	arg1	Scaffold					30:37	A Decellularized Human Limbal Scaffold	0:37	A Decellularized Human Limbal Scaffold for Limbal Stem Cell Niche Reconstruction.	0:80	A Decellularized Human Limbal Scaffold for Limbal Stem Cell Niche Reconstruction.
34576227	2	76	theme	niche-specific	396:409	arg1	structure					411:419	niche-specific structure	396:419	niche-specific structure	396:419	A scaffold with niche-specific structure and extracellular matrix (ECM) composition might have the advantage to improve long-term clinical outcomes, in particular for patients with severe damage or complete loss of the limbal niche tissue structure.
34576227	6	77	dep	studied	1058:1064	arg1	plating					1069:1075	plating	1069:1075	plating cultured LEPCs and limbal melanocytes (LMs)	1069:1119	The recellularization of these scaffolds was studied by plating cultured LEPCs and limbal melanocytes (LMs) or by allowing cells to migrate from the host tissue following a lamellar transplantation ex vivo.
34576227	6	77	dep	studied	1058:1064	arg1	allowing					1127:1134	allowing	1127:1134	allowing cells to migrate from the host tissue following a lamellar transplantation ex vivo	1127:1217	The recellularization of these scaffolds was studied by plating cultured LEPCs and limbal melanocytes (LMs) or by allowing cells to migrate from the host tissue following a lamellar transplantation ex vivo.
34576227	10	78	theme	biological	1679:1688	arg1	scaffold					1649:1656	the generated DHL scaffold	1631:1656	the generated DHL scaffold	1631:1656	Thus, the generated DHL scaffold could be a promising biological material as a carrier for the transplantation of LEPCs to treat LSCD.
34576227	10	78	theme	biological	1679:1688	arg1	material					1690:1697	a promising biological material	1667:1697	a promising biological material	1667:1697	Thus, the generated DHL scaffold could be a promising biological material as a carrier for the transplantation of LEPCs to treat LSCD.
34576227	5	79	theme	human	991:995	arg1	limbus					1005:1010	the human corneal limbus	987:1010	the human corneal limbus	987:1010	We evaluated the efficiency of decellularization and its effects on the ultrastructure and ECM composition of the human corneal limbus.
34576227	0	80	theme	Stem	50:53	arg1	Reconstruction					66:79	Limbal Stem Cell Niche Reconstruction	43:79	Limbal Stem Cell Niche Reconstruction	43:79	A Decellularized Human Limbal Scaffold for Limbal Stem Cell Niche Reconstruction.
34576227	1	81	theme	limbal	298:303	arg1	cell					310:313	limbal stem cell	298:313	limbal stem cell deficiency (LSCD)	298:331	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	5	82	theme	limbus	1005:1010	arg1	ultrastructure					949:962	ultrastructure	949:962	ultrastructure	949:962	We evaluated the efficiency of decellularization and its effects on the ultrastructure and ECM composition of the human corneal limbus.
34576227	5	82	theme	limbus	1005:1010	arg1	composition					972:982	ECM composition	968:982	ECM composition	968:982	We evaluated the efficiency of decellularization and its effects on the ultrastructure and ECM composition of the human corneal limbus.
34576227	2	83	theme	matrix	439:444	arg1	composition					452:462	extracellular matrix (ECM) composition	425:462	extracellular matrix (ECM) composition	425:462	A scaffold with niche-specific structure and extracellular matrix (ECM) composition might have the advantage to improve long-term clinical outcomes, in particular for patients with severe damage or complete loss of the limbal niche tissue structure.
34576227	4	84	theme	sodium	794:799	arg1	deoxycholate					801:812	sodium deoxycholate	794:812	sodium deoxycholate	794:812	Corneoscleral tissue was decellularized by sodium deoxycholate and deoxyribonuclease I in the presence or absence of dextran.
34576227	2	85	contain	have	470:473	arg1	scaffold					382:389	A scaffold	380:389	A scaffold with niche-specific structure and extracellular matrix (ECM) composition	380:462	A scaffold with niche-specific structure and extracellular matrix (ECM) composition might have the advantage to improve long-term clinical outcomes, in particular for patients with severe damage or complete loss of the limbal niche tissue structure.
34576227	2	85	contain	have	470:473	arg2	advantage					479:487	the advantage to improve long-term clinical outcomes	475:526	the advantage to improve long-term clinical outcomes	475:526	A scaffold with niche-specific structure and extracellular matrix (ECM) composition might have the advantage to improve long-term clinical outcomes, in particular for patients with severe damage or complete loss of the limbal niche tissue structure.
34576227	1	86	theme	cell	310:313	arg1	LSCD					327:330	LSCD	327:330	LSCD	327:330	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	1	86	theme	cell	310:313	arg1	deficiency					315:324	limbal stem cell deficiency	298:324	limbal stem cell deficiency (LSCD)	298:331	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	5	87	theme	ECM	968:970	arg1	composition					972:982	ECM composition	968:982	ECM composition	968:982	We evaluated the efficiency of decellularization and its effects on the ultrastructure and ECM composition of the human corneal limbus.
34576227	1	88	theme	established	206:216	arg1	transplantation					86:100	The transplantation	82:100	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel	82:198	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	1	88	theme	established	206:216	arg1	strategy					230:237	an established therapeutic strategy	203:237	an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD)	203:331	The transplantation of ex vivo expanded limbal epithelial progenitor cells (LEPCs) on amniotic membrane or fibrin gel is an established therapeutic strategy to regenerate the damaged corneal surface in patients with limbal stem cell deficiency (LSCD), but the long-term success rate is restricted.
34576227	3	89	theme	human	673:677	arg1	scaffold					708:715	a biomimetic scaffold	695:715	a biomimetic scaffold for the transplantation of LEPCs	695:748	Therefore, we evaluated the decellularized human limbus (DHL) as a biomimetic scaffold for the transplantation of LEPCs.
34576227	3	89	theme	human	673:677	arg1	DHL					687:689	DHL	687:689	DHL	687:689	Therefore, we evaluated the decellularized human limbus (DHL) as a biomimetic scaffold for the transplantation of LEPCs.
34576227	3	89	theme	human	673:677	arg1	limbus					679:684	the decellularized human limbus	654:684	the decellularized human limbus (DHL)	654:690	Therefore, we evaluated the decellularized human limbus (DHL) as a biomimetic scaffold for the transplantation of LEPCs.
34509521	6	0	theme	TGA	809:811	arg1	results					813:819	The SEM, FTIR, XRD and TGA results	786:819	The SEM, FTIR, XRD and TGA results	786:819	The SEM, FTIR, XRD and TGA results further confirmed the successful preparation of ZCCA.
34509521	5	1	theme	cellulose	680:688	arg1	aerogel					690:696	cellulose aerogel	680:696	cellulose aerogel	680:696	The Zeolitic imidazolate framework-8 (ZIF-8) was uniformly loaded onto the surface of cellulose aerogel by the in situ growth, and ZIF-8 Cattail Cellulose Aerogel (ZCCA) was finally prepared.
34509521	1	2	theme	extract	211:217	arg1	cellulose					219:227	extract cellulose	211:227	extract cellulose	211:227	In this study, cattail was researched as a natural cellulose source to extract cellulose.
34509521	5	3	theme	in	705:706	arg1	growth					713:718	the in situ growth	701:718	the in situ growth	701:718	The Zeolitic imidazolate framework-8 (ZIF-8) was uniformly loaded onto the surface of cellulose aerogel by the in situ growth, and ZIF-8 Cattail Cellulose Aerogel (ZCCA) was finally prepared.
34509521	8	4	theme	kinetic	1164:1170	arg1	model					1172:1176	the pseudo-second-order kinetic model	1140:1176	the pseudo-second-order kinetic model	1140:1176	The adsorption process followed the pseudo-second-order kinetic model and the Langmuir isotherm model.
34509521	7	5	theme	adsorption	1018:1027	arg1	capacity					1029:1036	the maximum adsorption capacity	1006:1036	the maximum adsorption capacity of enrofloxacin	1006:1052	Additionally, the results of the adsorption experiment showed that ZCCA had excellent adsorption performance for enrofloxacin, and the maximum adsorption capacity of enrofloxacin reached 172.09 mg·g-1 while showing good reusability.
34509521	9	6	theme	bonds	1386:1390	arg1	interaction					1362:1372	the interaction	1358:1372	the interaction of hydrogen bonds	1358:1390	Thermodynamic studies showed that the adsorption of enrofloxacin was a spontaneous endothermic reaction and that the adsorption mechanism involves the interaction of hydrogen bonds, electrostatic and π-π stacking.
34509521	9	6	theme	bonds	1386:1390	arg1	stacking					1415:1422	electrostatic and π-π stacking	1393:1422	electrostatic and π-π stacking	1393:1422	Thermodynamic studies showed that the adsorption of enrofloxacin was a spontaneous endothermic reaction and that the adsorption mechanism involves the interaction of hydrogen bonds, electrostatic and π-π stacking.
34509521	0	7	theme	aerogel	75:81	arg1	type					57:60	a novel type	49:60	a novel type of composite aerogel adsorbent for the removal of enrofloxacin in wastewater	49:137	Cattail fibers as source of cellulose to prepare a novel type of composite aerogel adsorbent for the removal of enrofloxacin in wastewater.
34509521	6	8	theme	XRD	801:803	arg1	results					813:819	The SEM, FTIR, XRD and TGA results	786:819	The SEM, FTIR, XRD and TGA results	786:819	The SEM, FTIR, XRD and TGA results further confirmed the successful preparation of ZCCA.
34509521	5	9	theme	Zeolitic	598:605	arg1	ZIF-8					632:636	ZIF-8	632:636	ZIF-8	632:636	The Zeolitic imidazolate framework-8 (ZIF-8) was uniformly loaded onto the surface of cellulose aerogel by the in situ growth, and ZIF-8 Cattail Cellulose Aerogel (ZCCA) was finally prepared.
34509521	5	9	theme	Zeolitic	598:605	arg1	framework-8					619:629	The Zeolitic imidazolate framework-8	594:629	The Zeolitic imidazolate framework-8 (ZIF-8)	594:637	The Zeolitic imidazolate framework-8 (ZIF-8) was uniformly loaded onto the surface of cellulose aerogel by the in situ growth, and ZIF-8 Cattail Cellulose Aerogel (ZCCA) was finally prepared.
34509521	3	10	attach	removed	407:413	arg2	lignin					382:387	lignin	382:387	lignin	382:387	The FTIR, SEM and XRD results indicated that hemicellulose and lignin were successfully removed from the CFs, and the content of cattail cellulose increased from 41.66 ± 1.11% to 89.72 ± 1.07%.
34509521	3	10	attach	removed	407:413	arg1	CFs					424:426	the CFs	420:426	the CFs	420:426	The FTIR, SEM and XRD results indicated that hemicellulose and lignin were successfully removed from the CFs, and the content of cattail cellulose increased from 41.66 ± 1.11% to 89.72 ± 1.07%.
34509521	3	10	attach	removed	407:413	arg2	hemicellulose					364:376	hemicellulose	364:376	hemicellulose	364:376	The FTIR, SEM and XRD results indicated that hemicellulose and lignin were successfully removed from the CFs, and the content of cattail cellulose increased from 41.66 ± 1.11% to 89.72 ± 1.07%.
34509521	2	11	theme	bleaching	251:259	arg1	treatments					261:270	Dewaxing, alkali and bleaching treatments	230:270	Dewaxing, alkali and bleaching treatments	230:270	Dewaxing, alkali and bleaching treatments were carried out for the cattail fibers (CFs).
34509521	9	12	theme	hydrogen	1377:1384	arg1	bonds					1386:1390	hydrogen bonds	1377:1390	hydrogen bonds	1377:1390	Thermodynamic studies showed that the adsorption of enrofloxacin was a spontaneous endothermic reaction and that the adsorption mechanism involves the interaction of hydrogen bonds, electrostatic and π-π stacking.
34509521	4	13	theme	cellulose	527:535	arg1	aerogel					537:543	cellulose aerogel	527:543	cellulose aerogel	527:543	Subsequently, cellulose aerogel was prepared by the extracted cattail cellulose.
34509521	0	14	theme	composite	65:73	arg1	aerogel					75:81	composite aerogel	65:81	composite aerogel	65:81	Cattail fibers as source of cellulose to prepare a novel type of composite aerogel adsorbent for the removal of enrofloxacin in wastewater.
34509521	5	15	theme	imidazolate	607:617	arg1	ZIF-8					632:636	ZIF-8	632:636	ZIF-8	632:636	The Zeolitic imidazolate framework-8 (ZIF-8) was uniformly loaded onto the surface of cellulose aerogel by the in situ growth, and ZIF-8 Cattail Cellulose Aerogel (ZCCA) was finally prepared.
34509521	5	15	theme	imidazolate	607:617	arg1	framework-8					619:629	The Zeolitic imidazolate framework-8	594:629	The Zeolitic imidazolate framework-8 (ZIF-8)	594:637	The Zeolitic imidazolate framework-8 (ZIF-8) was uniformly loaded onto the surface of cellulose aerogel by the in situ growth, and ZIF-8 Cattail Cellulose Aerogel (ZCCA) was finally prepared.
34509521	3	16	theme	41.66 ± 1.11	481:492	arg1	%					493:493	%	493:493	%	493:493	The FTIR, SEM and XRD results indicated that hemicellulose and lignin were successfully removed from the CFs, and the content of cattail cellulose increased from 41.66 ± 1.11% to 89.72 ± 1.07%.
34509521	8	17	theme	adsorption	1112:1121	arg1	process					1123:1129	The adsorption process	1108:1129	The adsorption process	1108:1129	The adsorption process followed the pseudo-second-order kinetic model and the Langmuir isotherm model.
34509521	6	18	theme	FTIR	795:798	arg1	results					813:819	The SEM, FTIR, XRD and TGA results	786:819	The SEM, FTIR, XRD and TGA results	786:819	The SEM, FTIR, XRD and TGA results further confirmed the successful preparation of ZCCA.
34509521	2	19	theme	alkali	240:245	arg1	treatments					261:270	Dewaxing, alkali and bleaching treatments	230:270	Dewaxing, alkali and bleaching treatments	230:270	Dewaxing, alkali and bleaching treatments were carried out for the cattail fibers (CFs).
34509521	0	20	attach	removal	101:107	arg2	enrofloxacin					112:123	enrofloxacin	112:123	enrofloxacin	112:123	Cattail fibers as source of cellulose to prepare a novel type of composite aerogel adsorbent for the removal of enrofloxacin in wastewater.
34509521	0	20	attach	removal	101:107	arg3	wastewater					128:137	wastewater	128:137	wastewater	128:137	Cattail fibers as source of cellulose to prepare a novel type of composite aerogel adsorbent for the removal of enrofloxacin in wastewater.
34509521	0	21	theme	adsorbent	83:91	arg1	type					57:60	a novel type	49:60	a novel type of composite aerogel adsorbent for the removal of enrofloxacin in wastewater	49:137	Cattail fibers as source of cellulose to prepare a novel type of composite aerogel adsorbent for the removal of enrofloxacin in wastewater.
34509521	8	22	theme	pseudo-second-order	1144:1162	arg1	model					1172:1176	the pseudo-second-order kinetic model	1140:1176	the pseudo-second-order kinetic model	1140:1176	The adsorption process followed the pseudo-second-order kinetic model and the Langmuir isotherm model.
34509521	0	23	theme	Cattail	0:6	arg1	fibers					8:13	Cattail fibers	0:13	Cattail fibers as source of cellulose	0:36	Cattail fibers as source of cellulose to prepare a novel type of composite aerogel adsorbent for the removal of enrofloxacin in wastewater.
34509521	7	24	contain	had	947:949	arg1	ZCCA					942:945	ZCCA	942:945	ZCCA	942:945	Additionally, the results of the adsorption experiment showed that ZCCA had excellent adsorption performance for enrofloxacin, and the maximum adsorption capacity of enrofloxacin reached 172.09 mg·g-1 while showing good reusability.
34509521	7	24	contain	had	947:949	arg2	performance					972:982	excellent adsorption performance	951:982	excellent adsorption performance for enrofloxacin	951:999	Additionally, the results of the adsorption experiment showed that ZCCA had excellent adsorption performance for enrofloxacin, and the maximum adsorption capacity of enrofloxacin reached 172.09 mg·g-1 while showing good reusability.
34509521	9	25	theme	π-π	1411:1413	arg1	stacking					1415:1422	electrostatic and π-π stacking	1393:1422	electrostatic and π-π stacking	1393:1422	Thermodynamic studies showed that the adsorption of enrofloxacin was a spontaneous endothermic reaction and that the adsorption mechanism involves the interaction of hydrogen bonds, electrostatic and π-π stacking.
34509521	9	25	theme	π-π	1411:1413	arg1	interaction					1362:1372	the interaction	1358:1372	the interaction of hydrogen bonds	1358:1390	Thermodynamic studies showed that the adsorption of enrofloxacin was a spontaneous endothermic reaction and that the adsorption mechanism involves the interaction of hydrogen bonds, electrostatic and π-π stacking.
34509521	5	26	theme	ZIF-8	725:729	arg1	ZCCA					758:761	ZCCA	758:761	ZCCA	758:761	The Zeolitic imidazolate framework-8 (ZIF-8) was uniformly loaded onto the surface of cellulose aerogel by the in situ growth, and ZIF-8 Cattail Cellulose Aerogel (ZCCA) was finally prepared.
34509521	5	26	theme	ZIF-8	725:729	arg1	Aerogel					749:755	ZIF-8 Cattail Cellulose Aerogel	725:755	ZIF-8 Cattail Cellulose Aerogel (ZCCA)	725:762	The Zeolitic imidazolate framework-8 (ZIF-8) was uniformly loaded onto the surface of cellulose aerogel by the in situ growth, and ZIF-8 Cattail Cellulose Aerogel (ZCCA) was finally prepared.
34509521	6	27	theme	SEM	790:792	arg1	results					813:819	The SEM, FTIR, XRD and TGA results	786:819	The SEM, FTIR, XRD and TGA results	786:819	The SEM, FTIR, XRD and TGA results further confirmed the successful preparation of ZCCA.
34509521	5	28	theme	aerogel	690:696	arg1	surface					669:675	the surface	665:675	the surface of cellulose aerogel	665:696	The Zeolitic imidazolate framework-8 (ZIF-8) was uniformly loaded onto the surface of cellulose aerogel by the in situ growth, and ZIF-8 Cattail Cellulose Aerogel (ZCCA) was finally prepared.
34509521	3	29	theme	89.72 ± 1.07	498:509	arg1	%					493:493	%	493:493	%	493:493	The FTIR, SEM and XRD results indicated that hemicellulose and lignin were successfully removed from the CFs, and the content of cattail cellulose increased from 41.66 ± 1.11% to 89.72 ± 1.07%.
34509521	9	30	theme	enrofloxacin	1263:1274	arg1	reaction					1306:1313	a spontaneous endothermic reaction	1280:1313	a spontaneous endothermic reaction	1280:1313	Thermodynamic studies showed that the adsorption of enrofloxacin was a spontaneous endothermic reaction and that the adsorption mechanism involves the interaction of hydrogen bonds, electrostatic and π-π stacking.
34509521	9	30	theme	enrofloxacin	1263:1274	arg1	adsorption					1249:1258	the adsorption	1245:1258	the adsorption of enrofloxacin	1245:1274	Thermodynamic studies showed that the adsorption of enrofloxacin was a spontaneous endothermic reaction and that the adsorption mechanism involves the interaction of hydrogen bonds, electrostatic and π-π stacking.
34509521	8	31	theme	isotherm	1195:1202	arg1	model					1204:1208	the Langmuir isotherm model	1182:1208	the Langmuir isotherm model	1182:1208	The adsorption process followed the pseudo-second-order kinetic model and the Langmuir isotherm model.
34509521	3	32	theme	SEM	329:331	arg1	results					341:347	The FTIR, SEM and XRD results	319:347	The FTIR, SEM and XRD results	319:347	The FTIR, SEM and XRD results indicated that hemicellulose and lignin were successfully removed from the CFs, and the content of cattail cellulose increased from 41.66 ± 1.11% to 89.72 ± 1.07%.
34509521	9	33	theme	spontaneous	1282:1292	arg1	reaction					1306:1313	a spontaneous endothermic reaction	1280:1313	a spontaneous endothermic reaction	1280:1313	Thermodynamic studies showed that the adsorption of enrofloxacin was a spontaneous endothermic reaction and that the adsorption mechanism involves the interaction of hydrogen bonds, electrostatic and π-π stacking.
34509521	9	33	theme	spontaneous	1282:1292	arg1	adsorption					1249:1258	the adsorption	1245:1258	the adsorption of enrofloxacin	1245:1274	Thermodynamic studies showed that the adsorption of enrofloxacin was a spontaneous endothermic reaction and that the adsorption mechanism involves the interaction of hydrogen bonds, electrostatic and π-π stacking.
34509521	8	34	theme	Langmuir	1186:1193	arg1	model					1204:1208	the Langmuir isotherm model	1182:1208	the Langmuir isotherm model	1182:1208	The adsorption process followed the pseudo-second-order kinetic model and the Langmuir isotherm model.
34509521	5	35	theme	Cellulose	739:747	arg1	ZCCA					758:761	ZCCA	758:761	ZCCA	758:761	The Zeolitic imidazolate framework-8 (ZIF-8) was uniformly loaded onto the surface of cellulose aerogel by the in situ growth, and ZIF-8 Cattail Cellulose Aerogel (ZCCA) was finally prepared.
34509521	5	35	theme	Cellulose	739:747	arg1	Aerogel					749:755	ZIF-8 Cattail Cellulose Aerogel	725:755	ZIF-8 Cattail Cellulose Aerogel (ZCCA)	725:762	The Zeolitic imidazolate framework-8 (ZIF-8) was uniformly loaded onto the surface of cellulose aerogel by the in situ growth, and ZIF-8 Cattail Cellulose Aerogel (ZCCA) was finally prepared.
34509521	5	36	theme	Cattail	731:737	arg1	ZCCA					758:761	ZCCA	758:761	ZCCA	758:761	The Zeolitic imidazolate framework-8 (ZIF-8) was uniformly loaded onto the surface of cellulose aerogel by the in situ growth, and ZIF-8 Cattail Cellulose Aerogel (ZCCA) was finally prepared.
34509521	5	36	theme	Cattail	731:737	arg1	Aerogel					749:755	ZIF-8 Cattail Cellulose Aerogel	725:755	ZIF-8 Cattail Cellulose Aerogel (ZCCA)	725:762	The Zeolitic imidazolate framework-8 (ZIF-8) was uniformly loaded onto the surface of cellulose aerogel by the in situ growth, and ZIF-8 Cattail Cellulose Aerogel (ZCCA) was finally prepared.
34509521	4	37	theme	extracted	565:573	arg1	cellulose					583:591	the extracted cattail cellulose	561:591	the extracted cattail cellulose	561:591	Subsequently, cellulose aerogel was prepared by the extracted cattail cellulose.
34509521	3	38	theme	FTIR	323:326	arg1	results					341:347	The FTIR, SEM and XRD results	319:347	The FTIR, SEM and XRD results	319:347	The FTIR, SEM and XRD results indicated that hemicellulose and lignin were successfully removed from the CFs, and the content of cattail cellulose increased from 41.66 ± 1.11% to 89.72 ± 1.07%.
34509521	7	39	theme	excellent	951:959	arg1	performance					972:982	excellent adsorption performance	951:982	excellent adsorption performance for enrofloxacin	951:999	Additionally, the results of the adsorption experiment showed that ZCCA had excellent adsorption performance for enrofloxacin, and the maximum adsorption capacity of enrofloxacin reached 172.09 mg·g-1 while showing good reusability.
34509521	3	40	theme	cellulose	456:464	arg1	content					437:443	the content	433:443	the content of cattail cellulose	433:464	The FTIR, SEM and XRD results indicated that hemicellulose and lignin were successfully removed from the CFs, and the content of cattail cellulose increased from 41.66 ± 1.11% to 89.72 ± 1.07%.
34509521	9	41	theme	electrostatic	1393:1405	arg1	stacking					1415:1422	electrostatic and π-π stacking	1393:1422	electrostatic and π-π stacking	1393:1422	Thermodynamic studies showed that the adsorption of enrofloxacin was a spontaneous endothermic reaction and that the adsorption mechanism involves the interaction of hydrogen bonds, electrostatic and π-π stacking.
34509521	9	41	theme	electrostatic	1393:1405	arg1	interaction					1362:1372	the interaction	1358:1372	the interaction of hydrogen bonds	1358:1390	Thermodynamic studies showed that the adsorption of enrofloxacin was a spontaneous endothermic reaction and that the adsorption mechanism involves the interaction of hydrogen bonds, electrostatic and π-π stacking.
34509521	0	42	theme	cellulose	28:36	arg1	source					18:23	source	18:23	source of cellulose	18:36	Cattail fibers as source of cellulose to prepare a novel type of composite aerogel adsorbent for the removal of enrofloxacin in wastewater.
34509521	5	43	dep	in	705:706	arg1	situ					708:711	situ	708:711	situ	708:711	The Zeolitic imidazolate framework-8 (ZIF-8) was uniformly loaded onto the surface of cellulose aerogel by the in situ growth, and ZIF-8 Cattail Cellulose Aerogel (ZCCA) was finally prepared.
34509521	0	44	theme	enrofloxacin	112:123	arg1	removal					101:107	the removal	97:107	the removal of enrofloxacin in wastewater	97:137	Cattail fibers as source of cellulose to prepare a novel type of composite aerogel adsorbent for the removal of enrofloxacin in wastewater.
34509521	6	45	theme	successful	843:852	arg1	preparation					854:864	the successful preparation	839:864	the successful preparation of ZCCA	839:872	The SEM, FTIR, XRD and TGA results further confirmed the successful preparation of ZCCA.
34509521	7	46	theme	adsorption	961:970	arg1	performance					972:982	excellent adsorption performance	951:982	excellent adsorption performance for enrofloxacin	951:999	Additionally, the results of the adsorption experiment showed that ZCCA had excellent adsorption performance for enrofloxacin, and the maximum adsorption capacity of enrofloxacin reached 172.09 mg·g-1 while showing good reusability.
34509521	9	47	theme	endothermic	1294:1304	arg1	reaction					1306:1313	a spontaneous endothermic reaction	1280:1313	a spontaneous endothermic reaction	1280:1313	Thermodynamic studies showed that the adsorption of enrofloxacin was a spontaneous endothermic reaction and that the adsorption mechanism involves the interaction of hydrogen bonds, electrostatic and π-π stacking.
34509521	9	47	theme	endothermic	1294:1304	arg1	adsorption					1249:1258	the adsorption	1245:1258	the adsorption of enrofloxacin	1245:1274	Thermodynamic studies showed that the adsorption of enrofloxacin was a spontaneous endothermic reaction and that the adsorption mechanism involves the interaction of hydrogen bonds, electrostatic and π-π stacking.
34509521	7	48	theme	adsorption	908:917	arg1	experiment					919:928	the adsorption experiment	904:928	the adsorption experiment	904:928	Additionally, the results of the adsorption experiment showed that ZCCA had excellent adsorption performance for enrofloxacin, and the maximum adsorption capacity of enrofloxacin reached 172.09 mg·g-1 while showing good reusability.
34509521	4	49	theme	cattail	575:581	arg1	cellulose					583:591	the extracted cattail cellulose	561:591	the extracted cattail cellulose	561:591	Subsequently, cellulose aerogel was prepared by the extracted cattail cellulose.
34509521	7	50	theme	enrofloxacin	1041:1052	arg1	capacity					1029:1036	the maximum adsorption capacity	1006:1036	the maximum adsorption capacity of enrofloxacin	1006:1052	Additionally, the results of the adsorption experiment showed that ZCCA had excellent adsorption performance for enrofloxacin, and the maximum adsorption capacity of enrofloxacin reached 172.09 mg·g-1 while showing good reusability.
34509521	6	51	theme	ZCCA	869:872	arg1	preparation					854:864	the successful preparation	839:864	the successful preparation of ZCCA	839:872	The SEM, FTIR, XRD and TGA results further confirmed the successful preparation of ZCCA.
34509521	7	52	theme	experiment	919:928	arg1	results					893:899	the results	889:899	the results of the adsorption experiment	889:928	Additionally, the results of the adsorption experiment showed that ZCCA had excellent adsorption performance for enrofloxacin, and the maximum adsorption capacity of enrofloxacin reached 172.09 mg·g-1 while showing good reusability.
34509521	3	53	theme	XRD	337:339	arg1	results					341:347	The FTIR, SEM and XRD results	319:347	The FTIR, SEM and XRD results	319:347	The FTIR, SEM and XRD results indicated that hemicellulose and lignin were successfully removed from the CFs, and the content of cattail cellulose increased from 41.66 ± 1.11% to 89.72 ± 1.07%.
34509521	9	54	theme	adsorption	1328:1337	arg1	mechanism					1339:1347	the adsorption mechanism	1324:1347	the adsorption mechanism	1324:1347	Thermodynamic studies showed that the adsorption of enrofloxacin was a spontaneous endothermic reaction and that the adsorption mechanism involves the interaction of hydrogen bonds, electrostatic and π-π stacking.
34509521	2	55	theme	cattail	297:303	arg1	CFs					313:315	CFs	313:315	CFs	313:315	Dewaxing, alkali and bleaching treatments were carried out for the cattail fibers (CFs).
34509521	2	55	theme	cattail	297:303	arg1	fibers					305:310	the cattail fibers	293:310	the cattail fibers (CFs)	293:316	Dewaxing, alkali and bleaching treatments were carried out for the cattail fibers (CFs).
34509521	2	56	theme	Dewaxing	230:237	arg1	treatments					261:270	Dewaxing, alkali and bleaching treatments	230:270	Dewaxing, alkali and bleaching treatments	230:270	Dewaxing, alkali and bleaching treatments were carried out for the cattail fibers (CFs).
34509521	1	57	theme	natural	183:189	arg1	source					201:206	a natural cellulose source	181:206	a natural cellulose source to extract cellulose	181:227	In this study, cattail was researched as a natural cellulose source to extract cellulose.
34509521	1	57	theme	natural	183:189	arg1	cattail					155:161	cattail	155:161	cattail	155:161	In this study, cattail was researched as a natural cellulose source to extract cellulose.
34509521	9	58	theme	Thermodynamic	1211:1223	arg1	studies					1225:1231	Thermodynamic studies	1211:1231	Thermodynamic studies	1211:1231	Thermodynamic studies showed that the adsorption of enrofloxacin was a spontaneous endothermic reaction and that the adsorption mechanism involves the interaction of hydrogen bonds, electrostatic and π-π stacking.
34509521	7	59	theme	maximum	1010:1016	arg1	capacity					1029:1036	the maximum adsorption capacity	1006:1036	the maximum adsorption capacity of enrofloxacin	1006:1052	Additionally, the results of the adsorption experiment showed that ZCCA had excellent adsorption performance for enrofloxacin, and the maximum adsorption capacity of enrofloxacin reached 172.09 mg·g-1 while showing good reusability.
34509521	1	60	theme	cellulose	191:199	arg1	source					201:206	a natural cellulose source	181:206	a natural cellulose source to extract cellulose	181:227	In this study, cattail was researched as a natural cellulose source to extract cellulose.
34509521	1	60	theme	cellulose	191:199	arg1	cattail					155:161	cattail	155:161	cattail	155:161	In this study, cattail was researched as a natural cellulose source to extract cellulose.
34509521	0	61	theme	novel	51:55	arg1	type					57:60	a novel type	49:60	a novel type of composite aerogel adsorbent for the removal of enrofloxacin in wastewater	49:137	Cattail fibers as source of cellulose to prepare a novel type of composite aerogel adsorbent for the removal of enrofloxacin in wastewater.
34509521	3	62	dep	%	493:493	arg1	to					495:496	to	495:496	to	495:496	The FTIR, SEM and XRD results indicated that hemicellulose and lignin were successfully removed from the CFs, and the content of cattail cellulose increased from 41.66 ± 1.11% to 89.72 ± 1.07%.
34509521	0	63	from	removal	101:107	arg1	wastewater					128:137	wastewater	128:137	wastewater	128:137	Cattail fibers as source of cellulose to prepare a novel type of composite aerogel adsorbent for the removal of enrofloxacin in wastewater.
34509521	7	64	theme	good	1090:1093	arg1	reusability					1095:1105	good reusability	1090:1105	good reusability	1090:1105	Additionally, the results of the adsorption experiment showed that ZCCA had excellent adsorption performance for enrofloxacin, and the maximum adsorption capacity of enrofloxacin reached 172.09 mg·g-1 while showing good reusability.
34509521	3	65	theme	cattail	448:454	arg1	cellulose					456:464	cattail cellulose	448:464	cattail cellulose	448:464	The FTIR, SEM and XRD results indicated that hemicellulose and lignin were successfully removed from the CFs, and the content of cattail cellulose increased from 41.66 ± 1.11% to 89.72 ± 1.07%.
32423679	0	0	theme	high	89:92	arg1	activity					105:112	very high volumetric activity	84:112	very high volumetric activity using octyl-agarose beads	84:138	Use of polyethylenimine to produce immobilized lipase multilayers biocatalysts with very high volumetric activity using octyl-agarose beads: Avoiding enzyme release during multilayer production.
32423679	5	1	theme	enzyme	1092:1097	arg1	activity					1099:1106	enzyme activity	1092:1106	enzyme activity	1092:1106	The enzyme modifications produced more active preparations in some cases while in other cases, the effect of the modifications was negative for enzyme activity.
32423679	1	2	dep	antarctica	312:321	arg1	CALB					333:336	CALB	333:336	CALB	333:336	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	1	2	dep	antarctica	312:321	arg1	CALA					324:327	CALA	324:327	CALA	324:327	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	3	3	theme	large	802:806	arg1	percentage					808:817	a large percentage	800:817	a large percentage of the PEI-immobilized enzyme	800:847	Except when using LEU, when the two-layer biocatalysts, a large percentage of the PEI-immobilized enzyme was released when a new batch of PEI was added.
32423679	8	4	theme	enzyme	1449:1454	arg1	layers					1456:1461	new enzyme layers	1445:1461	new enzyme layers	1445:1461	However, as the substrate diffusion problems increased when new enzyme layers were added, even a decrease in enzyme activity with some substrates was found after increasing the number of enzyme layers.
32423679	2	5	theme	enzyme	616:621	arg1	layer					623:627	a new enzyme layer	610:627	a new enzyme layer	610:627	The enzymes were initially immobilized via interfacial activation on octyl-agarose beads, treated with polyethylenimine (PEI) and a new enzyme layer was immobilized on the octyl-enzyme-PEI composite by ion exchange, producing octyl-enzyme-PEI-enzyme biocatalysts.
32423679	8	6	theme	enzyme	1572:1577	arg1	layers					1579:1584	enzyme layers	1572:1584	enzyme layers	1572:1584	However, as the substrate diffusion problems increased when new enzyme layers were added, even a decrease in enzyme activity with some substrates was found after increasing the number of enzyme layers.
32423679	7	7	from	active	1299:1304	arg1	cases					1257:1261	all cases	1253:1261	all cases	1253:1261	In all cases, the 3-layer biocatalysts were more active than the single- or bi-layer biocatalysts with some of the assayed substrates.
32423679	2	8	theme	new	612:614	arg1	layer					623:627	a new enzyme layer	610:627	a new enzyme layer	610:627	The enzymes were initially immobilized via interfacial activation on octyl-agarose beads, treated with polyethylenimine (PEI) and a new enzyme layer was immobilized on the octyl-enzyme-PEI composite by ion exchange, producing octyl-enzyme-PEI-enzyme biocatalysts.
32423679	2	9	theme	ion	682:684	arg1	exchange					686:693	ion exchange	682:693	ion exchange	682:693	The enzymes were initially immobilized via interfacial activation on octyl-agarose beads, treated with polyethylenimine (PEI) and a new enzyme layer was immobilized on the octyl-enzyme-PEI composite by ion exchange, producing octyl-enzyme-PEI-enzyme biocatalysts.
32423679	0	10	theme	volumetric	94:103	arg1	activity					105:112	very high volumetric activity	84:112	very high volumetric activity using octyl-agarose beads	84:138	Use of polyethylenimine to produce immobilized lipase multilayers biocatalysts with very high volumetric activity using octyl-agarose beads: Avoiding enzyme release during multilayer production.
32423679	1	11	from	antarctica	312:321	arg1	lipases					344:350	the lipases	340:350	the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL)	340:413	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	1	11	from	antarctica	312:321	arg1	A					291:291	A	291:291	A	291:291	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	1	11	from	antarctica	312:321	arg1	Ultra					467:471	the artificial chimeric phospholipase Lecitase Ultra	420:471	the artificial chimeric phospholipase Lecitase Ultra (LEU)	420:477	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	1	11	from	antarctica	312:321	arg1	B					297:297	B	297:297	B	297:297	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	1	11	from	antarctica	312:321	arg1	LEU					474:476	LEU	474:476	LEU	474:476	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	1	11	from	antarctica	312:321	arg1	lipases					283:289	lipases A and B	283:297	lipases A and B from Candida antarctica (CALA and CALB)	283:337	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	1	12	from	miehei	368:373	arg1	lipases					344:350	the lipases	340:350	the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL)	340:413	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	1	12	from	miehei	368:373	arg1	A					291:291	A	291:291	A	291:291	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	1	12	from	miehei	368:373	arg1	Ultra					467:471	the artificial chimeric phospholipase Lecitase Ultra	420:471	the artificial chimeric phospholipase Lecitase Ultra (LEU)	420:477	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	1	12	from	miehei	368:373	arg1	B					297:297	B	297:297	B	297:297	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	1	12	from	miehei	368:373	arg1	LEU					474:476	LEU	474:476	LEU	474:476	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	1	12	from	miehei	368:373	arg1	lipases					283:289	lipases A and B	283:297	lipases A and B from Candida antarctica (CALA and CALB)	283:337	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	5	13	theme	modifications	1061:1073	arg1	negative					1079:1086	negative	1079:1086	negative	1079:1086	The enzyme modifications produced more active preparations in some cases while in other cases, the effect of the modifications was negative for enzyme activity.
32423679	5	13	theme	modifications	1061:1073	arg1	effect					1047:1052	the effect	1043:1052	the effect of the modifications	1043:1073	The enzyme modifications produced more active preparations in some cases while in other cases, the effect of the modifications was negative for enzyme activity.
32423679	6	14	theme	ion	1236:1238	arg1	exchange					1240:1247	ion exchange	1236:1247	ion exchange	1236:1247	These effects of the enzymes modifications were also different when the enzyme was immobilized by interfacial activation or by ion exchange.
32423679	0	15	with	biocatalysts	66:77	arg1	activity					105:112	very high volumetric activity	84:112	very high volumetric activity using octyl-agarose beads	84:138	Use of polyethylenimine to produce immobilized lipase multilayers biocatalysts with very high volumetric activity using octyl-agarose beads: Avoiding enzyme release during multilayer production.
32423679	6	16	theme	modifications	1138:1150	arg1	effects					1115:1121	These effects	1109:1121	These effects of the enzymes modifications	1109:1150	These effects of the enzymes modifications were also different when the enzyme was immobilized by interfacial activation or by ion exchange.
32423679	3	17	theme	PEI	882:884	arg1	batch					873:877	a new batch	867:877	a new batch of PEI	867:884	Except when using LEU, when the two-layer biocatalysts, a large percentage of the PEI-immobilized enzyme was released when a new batch of PEI was added.
32423679	8	18	theme	layers	1579:1584	arg1	number					1562:1567	the number	1558:1567	the number of enzyme layers	1558:1584	However, as the substrate diffusion problems increased when new enzyme layers were added, even a decrease in enzyme activity with some substrates was found after increasing the number of enzyme layers.
32423679	5	19	theme	enzyme	952:957	arg1	modifications					959:971	The enzyme modifications	948:971	The enzyme modifications	948:971	The enzyme modifications produced more active preparations in some cases while in other cases, the effect of the modifications was negative for enzyme activity.
32423679	3	20	theme	new	869:871	arg1	batch					873:877	a new batch	867:877	a new batch of PEI	867:884	Except when using LEU, when the two-layer biocatalysts, a large percentage of the PEI-immobilized enzyme was released when a new batch of PEI was added.
32423679	0	21	theme	octyl-agarose	120:132	arg1	beads					134:138	octyl-agarose beads	120:138	octyl-agarose beads	120:138	Use of polyethylenimine to produce immobilized lipase multilayers biocatalysts with very high volumetric activity using octyl-agarose beads: Avoiding enzyme release during multilayer production.
32423679	1	22	theme	Rhizomucor	357:366	arg1	RML					376:378	RML	376:378	RML	376:378	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	1	22	theme	Rhizomucor	357:366	arg1	miehei					368:373	Rhizomucor miehei	357:373	Rhizomucor miehei (RML)	357:379	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	1	23	theme	artificial	424:433	arg1	LEU					474:476	LEU	474:476	LEU	474:476	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	1	23	theme	artificial	424:433	arg1	Ultra					467:471	the artificial chimeric phospholipase Lecitase Ultra	420:471	the artificial chimeric phospholipase Lecitase Ultra (LEU)	420:477	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	7	24	theme	assayed	1365:1371	arg1	substrates					1373:1382	the assayed substrates	1361:1382	the assayed substrates	1361:1382	In all cases, the 3-layer biocatalysts were more active than the single- or bi-layer biocatalysts with some of the assayed substrates.
32423679	2	25	theme	octyl-enzyme-PEI-enzyme	706:728	arg1	biocatalysts					730:741	octyl-enzyme-PEI-enzyme biocatalysts	706:741	octyl-enzyme-PEI-enzyme biocatalysts	706:741	The enzymes were initially immobilized via interfacial activation on octyl-agarose beads, treated with polyethylenimine (PEI) and a new enzyme layer was immobilized on the octyl-enzyme-PEI composite by ion exchange, producing octyl-enzyme-PEI-enzyme biocatalysts.
32423679	5	26	from	negative	1079:1086	arg1	cases					1036:1040	other cases	1030:1040	other cases	1030:1040	The enzyme modifications produced more active preparations in some cases while in other cases, the effect of the modifications was negative for enzyme activity.
32423679	1	27	theme	chimeric	435:442	arg1	LEU					474:476	LEU	474:476	LEU	474:476	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	1	27	theme	chimeric	435:442	arg1	Ultra					467:471	the artificial chimeric phospholipase Lecitase Ultra	420:471	the artificial chimeric phospholipase Lecitase Ultra (LEU)	420:477	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	8	28	theme	enzyme	1494:1499	arg1	activity					1501:1508	enzyme activity	1494:1508	enzyme activity	1494:1508	However, as the substrate diffusion problems increased when new enzyme layers were added, even a decrease in enzyme activity with some substrates was found after increasing the number of enzyme layers.
32423679	1	29	theme	phospholipase	444:456	arg1	LEU					474:476	LEU	474:476	LEU	474:476	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	1	29	theme	phospholipase	444:456	arg1	Ultra					467:471	the artificial chimeric phospholipase Lecitase Ultra	420:471	the artificial chimeric phospholipase Lecitase Ultra (LEU)	420:477	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	6	30	theme	interfacial	1207:1217	arg1	activation					1219:1228	interfacial activation	1207:1228	interfacial activation	1207:1228	These effects of the enzymes modifications were also different when the enzyme was immobilized by interfacial activation or by ion exchange.
32423679	0	31	theme	polyethylenimine	7:22	arg1	Use					0:2	Use	0:2	Use of polyethylenimine	0:22	Use of polyethylenimine to produce immobilized lipase multilayers biocatalysts with very high volumetric activity using octyl-agarose beads: Avoiding enzyme release during multilayer production.
32423679	8	32	theme	substrate	1401:1409	arg1	problems					1421:1428	the substrate diffusion problems	1397:1428	the substrate diffusion problems	1397:1428	However, as the substrate diffusion problems increased when new enzyme layers were added, even a decrease in enzyme activity with some substrates was found after increasing the number of enzyme layers.
32423679	7	33	theme	3-layer	1268:1274	arg1	biocatalysts					1276:1287	the 3-layer biocatalysts	1264:1287	the 3-layer biocatalysts	1264:1287	In all cases, the 3-layer biocatalysts were more active than the single- or bi-layer biocatalysts with some of the assayed substrates.
32423679	7	33	theme	3-layer	1268:1274	arg1	active					1299:1304	active	1299:1304	active	1299:1304	In all cases, the 3-layer biocatalysts were more active than the single- or bi-layer biocatalysts with some of the assayed substrates.
32423679	0	34	theme	immobilized	35:45	arg1	biocatalysts					66:77	immobilized lipase multilayers biocatalysts	35:77	immobilized lipase multilayers biocatalysts with very high volumetric activity using octyl-agarose beads	35:138	Use of polyethylenimine to produce immobilized lipase multilayers biocatalysts with very high volumetric activity using octyl-agarose beads: Avoiding enzyme release during multilayer production.
32423679	0	35	theme	enzyme	150:155	arg1	release					157:163	Avoiding enzyme release	141:163	Avoiding enzyme release during multilayer production	141:192	Use of polyethylenimine to produce immobilized lipase multilayers biocatalysts with very high volumetric activity using octyl-agarose beads: Avoiding enzyme release during multilayer production.
32423679	1	36	dep	strategy	197:204	arg1	obtain					209:214	obtain	209:214	to obtain biocatalysts formed by three enzyme layers	206:257	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	8	37	from	decrease	1482:1489	arg1	activity					1501:1508	enzyme activity	1494:1508	enzyme activity	1494:1508	However, as the substrate diffusion problems increased when new enzyme layers were added, even a decrease in enzyme activity with some substrates was found after increasing the number of enzyme layers.
32423679	1	38	theme	Candida	304:310	arg1	antarctica					312:321	Candida antarctica	304:321	Candida antarctica (CALA and CALB)	304:337	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	0	39	theme	Avoiding	141:148	arg1	release					157:163	Avoiding enzyme release	141:163	Avoiding enzyme release during multilayer production	141:192	Use of polyethylenimine to produce immobilized lipase multilayers biocatalysts with very high volumetric activity using octyl-agarose beads: Avoiding enzyme release during multilayer production.
32423679	1	40	theme	Lecitase	458:465	arg1	LEU					474:476	LEU	474:476	LEU	474:476	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	1	40	theme	Lecitase	458:465	arg1	Ultra					467:471	the artificial chimeric phospholipase Lecitase Ultra	420:471	the artificial chimeric phospholipase Lecitase Ultra (LEU)	420:477	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	0	41	theme	multilayers	54:64	arg1	biocatalysts					66:77	immobilized lipase multilayers biocatalysts	35:77	immobilized lipase multilayers biocatalysts with very high volumetric activity using octyl-agarose beads	35:138	Use of polyethylenimine to produce immobilized lipase multilayers biocatalysts with very high volumetric activity using octyl-agarose beads: Avoiding enzyme release during multilayer production.
32423679	2	42	theme	octyl-agarose	549:561	arg1	beads					563:567	octyl-agarose beads	549:567	octyl-agarose beads	549:567	The enzymes were initially immobilized via interfacial activation on octyl-agarose beads, treated with polyethylenimine (PEI) and a new enzyme layer was immobilized on the octyl-enzyme-PEI composite by ion exchange, producing octyl-enzyme-PEI-enzyme biocatalysts.
32423679	8	43	theme	diffusion	1411:1419	arg1	problems					1421:1428	the substrate diffusion problems	1397:1428	the substrate diffusion problems	1397:1428	However, as the substrate diffusion problems increased when new enzyme layers were added, even a decrease in enzyme activity with some substrates was found after increasing the number of enzyme layers.
32423679	7	44	theme	bi-layer	1326:1333	arg1	biocatalysts					1335:1346	the single- or bi-layer biocatalysts	1311:1346	the single- or bi-layer biocatalysts with some of the assayed substrates	1311:1382	In all cases, the 3-layer biocatalysts were more active than the single- or bi-layer biocatalysts with some of the assayed substrates.
32423679	0	45	theme	lipase	47:52	arg1	biocatalysts					66:77	immobilized lipase multilayers biocatalysts	35:77	immobilized lipase multilayers biocatalysts with very high volumetric activity using octyl-agarose beads	35:138	Use of polyethylenimine to produce immobilized lipase multilayers biocatalysts with very high volumetric activity using octyl-agarose beads: Avoiding enzyme release during multilayer production.
32423679	2	46	theme	interfacial	523:533	arg1	activation					535:544	interfacial activation	523:544	interfacial activation	523:544	The enzymes were initially immobilized via interfacial activation on octyl-agarose beads, treated with polyethylenimine (PEI) and a new enzyme layer was immobilized on the octyl-enzyme-PEI composite by ion exchange, producing octyl-enzyme-PEI-enzyme biocatalysts.
32423679	0	47	dep	produce	27:33	arg1	release					157:163	Avoiding enzyme release	141:163	Avoiding enzyme release during multilayer production	141:192	Use of polyethylenimine to produce immobilized lipase multilayers biocatalysts with very high volumetric activity using octyl-agarose beads: Avoiding enzyme release during multilayer production.
32423679	2	48	theme	octyl-enzyme-PEI	652:667	arg1	composite					669:677	the octyl-enzyme-PEI composite	648:677	the octyl-enzyme-PEI composite	648:677	The enzymes were initially immobilized via interfacial activation on octyl-agarose beads, treated with polyethylenimine (PEI) and a new enzyme layer was immobilized on the octyl-enzyme-PEI composite by ion exchange, producing octyl-enzyme-PEI-enzyme biocatalysts.
32423679	3	49	theme	PEI-immobilized	826:840	arg1	enzyme					842:847	the PEI-immobilized enzyme	822:847	the PEI-immobilized enzyme	822:847	Except when using LEU, when the two-layer biocatalysts, a large percentage of the PEI-immobilized enzyme was released when a new batch of PEI was added.
32423679	1	50	theme	Thermomyces	385:395	arg1	TLL					410:412	TLL	410:412	TLL	410:412	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	1	50	theme	Thermomyces	385:395	arg1	lanuginosus					397:407	Thermomyces lanuginosus	385:407	Thermomyces lanuginosus (TLL)	385:413	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	7	51	theme	single-	1315:1321	arg1	biocatalysts					1335:1346	the single- or bi-layer biocatalysts	1311:1346	the single- or bi-layer biocatalysts with some of the assayed substrates	1311:1382	In all cases, the 3-layer biocatalysts were more active than the single- or bi-layer biocatalysts with some of the assayed substrates.
32423679	8	52	with	decrease	1482:1489	arg1	substrates					1520:1529	some substrates	1515:1529	some substrates	1515:1529	However, as the substrate diffusion problems increased when new enzyme layers were added, even a decrease in enzyme activity with some substrates was found after increasing the number of enzyme layers.
32423679	3	53	theme	enzyme	842:847	arg1	percentage					808:817	a large percentage	800:817	a large percentage of the PEI-immobilized enzyme	800:847	Except when using LEU, when the two-layer biocatalysts, a large percentage of the PEI-immobilized enzyme was released when a new batch of PEI was added.
32423679	3	54	theme	two-layer	776:784	arg1	biocatalysts					786:797	the two-layer biocatalysts	772:797	the two-layer biocatalysts	772:797	Except when using LEU, when the two-layer biocatalysts, a large percentage of the PEI-immobilized enzyme was released when a new batch of PEI was added.
32423679	0	55	theme	multilayer	172:181	arg1	production					183:192	multilayer production	172:192	multilayer production	172:192	Use of polyethylenimine to produce immobilized lipase multilayers biocatalysts with very high volumetric activity using octyl-agarose beads: Avoiding enzyme release during multilayer production.
32423679	5	56	theme	other	1030:1034	arg1	cases					1036:1040	other cases	1030:1040	other cases	1030:1040	The enzyme modifications produced more active preparations in some cases while in other cases, the effect of the modifications was negative for enzyme activity.
32423679	7	57	from	cases	1257:1261	arg1	biocatalysts					1276:1287	the 3-layer biocatalysts	1264:1287	the 3-layer biocatalysts	1264:1287	In all cases, the 3-layer biocatalysts were more active than the single- or bi-layer biocatalysts with some of the assayed substrates.
32423679	7	57	from	cases	1257:1261	arg1	active					1299:1304	active	1299:1304	active	1299:1304	In all cases, the 3-layer biocatalysts were more active than the single- or bi-layer biocatalysts with some of the assayed substrates.
32423679	1	58	dep	lipases	283:289	arg1	B					297:297	B	297:297	B	297:297	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	1	58	dep	lipases	283:289	arg1	A					291:291	A	291:291	A	291:291	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	1	58	dep	lipases	283:289	arg1	lipases					283:289	lipases A and B	283:297	lipases A and B from Candida antarctica (CALA and CALB)	283:337	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	7	59	with	biocatalysts	1335:1346	arg1	substrates					1373:1382	the assayed substrates	1361:1382	the assayed substrates	1361:1382	In all cases, the 3-layer biocatalysts were more active than the single- or bi-layer biocatalysts with some of the assayed substrates.
32423679	7	59	with	biocatalysts	1335:1346	arg1	some					1353:1356	some	1353:1356	some	1353:1356	In all cases, the 3-layer biocatalysts were more active than the single- or bi-layer biocatalysts with some of the assayed substrates.
32423679	1	60	theme	enzyme	245:250	arg1	layers					252:257	three enzyme layers	239:257	three enzyme layers	239:257	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	1	61	from	lanuginosus	397:407	arg1	lipases					344:350	the lipases	340:350	the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL)	340:413	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	1	61	from	lanuginosus	397:407	arg1	A					291:291	A	291:291	A	291:291	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	1	61	from	lanuginosus	397:407	arg1	Ultra					467:471	the artificial chimeric phospholipase Lecitase Ultra	420:471	the artificial chimeric phospholipase Lecitase Ultra (LEU)	420:477	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	1	61	from	lanuginosus	397:407	arg1	B					297:297	B	297:297	B	297:297	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	1	61	from	lanuginosus	397:407	arg1	LEU					474:476	LEU	474:476	LEU	474:476	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	1	61	from	lanuginosus	397:407	arg1	lipases					283:289	lipases A and B	283:297	lipases A and B from Candida antarctica (CALA and CALB)	283:337	A strategy to obtain biocatalysts formed by three enzyme layers has been designed using lipases A and B from Candida antarctica (CALA and CALB), the lipases from Rhizomucor miehei (RML) and Thermomyces lanuginosus (TLL), and the artificial chimeric phospholipase Lecitase Ultra (LEU).
32423679	8	62	theme	new	1445:1447	arg1	layers					1456:1461	new enzyme layers	1445:1461	new enzyme layers	1445:1461	However, as the substrate diffusion problems increased when new enzyme layers were added, even a decrease in enzyme activity with some substrates was found after increasing the number of enzyme layers.
32423679	5	63	theme	more	982:985	arg1	preparations					994:1005	more active preparations	982:1005	more active preparations	982:1005	The enzyme modifications produced more active preparations in some cases while in other cases, the effect of the modifications was negative for enzyme activity.
32423679	6	64	theme	enzymes	1130:1136	arg1	modifications					1138:1150	the enzymes modifications	1126:1150	the enzymes modifications	1126:1150	These effects of the enzymes modifications were also different when the enzyme was immobilized by interfacial activation or by ion exchange.
32423679	5	65	from	cases	1036:1040	arg1	negative					1079:1086	negative	1079:1086	negative	1079:1086	The enzyme modifications produced more active preparations in some cases while in other cases, the effect of the modifications was negative for enzyme activity.
32423679	5	65	from	cases	1036:1040	arg1	effect					1047:1052	the effect	1043:1052	the effect of the modifications	1043:1073	The enzyme modifications produced more active preparations in some cases while in other cases, the effect of the modifications was negative for enzyme activity.
32423679	5	66	theme	active	987:992	arg1	preparations					994:1005	more active preparations	982:1005	more active preparations	982:1005	The enzyme modifications produced more active preparations in some cases while in other cases, the effect of the modifications was negative for enzyme activity.
32423679	4	67	theme	glutaraldehyde	919:932	arg1	crosslinking					934:945	glutaraldehyde crosslinking	919:945	glutaraldehyde crosslinking	919:945	This was prevented by glutaraldehyde crosslinking.
34340308	5	0	theme	sensor	965:970	arg1	scaffold					972:979	the sensor scaffold	961:979	the sensor scaffold	961:979	The superhydrophilicity and the capillarity of the synthesized titanium dioxide nanotubes, when incorporated into the alginate matrix, facilitate the rapid transfer of the artificial sweat components throughout the sensor scaffold, decreasing the detection times.
34340308	6	1	theme	paper	1224:1228	arg1	substrate					1230:1238	the paper substrate	1220:1238	the paper substrate	1220:1238	Moreover, the scaffold was integrated on a cellulose paper to demonstrate the adaptability of the material to other matrixes, obtaining fast and homogeneous colorimetric detection of lactate and glucose in the paper substrate when image analysis was performed.
34340308	1	2	theme	enzyme-immobilized	166:183	arg1	biosensors					193:202	enzyme-immobilized optical biosensors	166:202	enzyme-immobilized optical biosensors	166:202	Versatile sensing matrixes are essential for the development of enzyme-immobilized optical biosensors.
34340308	7	3	from	avenues	1324:1330	arg1	development					1339:1349	the development	1335:1349	the development of paper-based sensor devices	1335:1379	The properties of this new composite provide new avenues in the development of paper-based sensor devices.
34340308	1	4	theme	biosensors	193:202	arg1	development					151:161	the development	147:161	the development of enzyme-immobilized optical biosensors	147:202	Versatile sensing matrixes are essential for the development of enzyme-immobilized optical biosensors.
34340308	5	5	theme	detection	997:1005	arg1	times					1007:1011	the detection times	993:1011	the detection times	993:1011	The superhydrophilicity and the capillarity of the synthesized titanium dioxide nanotubes, when incorporated into the alginate matrix, facilitate the rapid transfer of the artificial sweat components throughout the sensor scaffold, decreasing the detection times.
34340308	2	6	theme	biomarkers	324:333	arg1	detection					305:313	the detection	301:313	the detection of sweat biomarkers, lactate, and glucose in artificial sweat	301:375	A novel three-dimensional titanium dioxide nanotubes/alginate hydrogel scaffold is proposed for the detection of sweat biomarkers, lactate, and glucose in artificial sweat.
34340308	5	7	theme	superhydrophilicity	754:772	arg1	nanotubes					830:838	The superhydrophilicity and the capillarity of the synthesized titanium dioxide nanotubes	750:838	The superhydrophilicity and the capillarity of the synthesized titanium dioxide nanotubes	750:838	The superhydrophilicity and the capillarity of the synthesized titanium dioxide nanotubes, when incorporated into the alginate matrix, facilitate the rapid transfer of the artificial sweat components throughout the sensor scaffold, decreasing the detection times.
34340308	5	8	theme	alginate	868:875	arg1	matrix					877:882	the alginate matrix	864:882	the alginate matrix	864:882	The superhydrophilicity and the capillarity of the synthesized titanium dioxide nanotubes, when incorporated into the alginate matrix, facilitate the rapid transfer of the artificial sweat components throughout the sensor scaffold, decreasing the detection times.
34340308	5	9	theme	rapid	900:904	arg1	transfer					906:913	the rapid transfer	896:913	the rapid transfer of the artificial sweat components	896:948	The superhydrophilicity and the capillarity of the synthesized titanium dioxide nanotubes, when incorporated into the alginate matrix, facilitate the rapid transfer of the artificial sweat components throughout the sensor scaffold, decreasing the detection times.
34340308	8	10	theme	titanium	1529:1536	arg1	dioxide					1538:1544	the titanium dioxide	1525:1544	the titanium dioxide	1525:1544	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds provide a prospective opportunity for integration into wearable devices.
34340308	4	11	dep	lactate	669:675	arg1	biomarkers					689:698	biomarkers	689:698	biomarkers	689:698	Rapid colorimetric detection (blue color optical signal) was carried out for both lactate and glucose biomarkers in artificial sweat at 4 and 6 min, respectively.
34340308	2	12	theme	titanium	231:238	arg1	dioxide					240:246	A novel three-dimensional titanium dioxide	205:246	A novel three-dimensional titanium dioxide nanotubes/alginate hydrogel scaffold	205:283	A novel three-dimensional titanium dioxide nanotubes/alginate hydrogel scaffold is proposed for the detection of sweat biomarkers, lactate, and glucose in artificial sweat.
34340308	1	13	theme	sensing	112:118	arg1	matrixes					120:127	Versatile sensing matrixes	102:127	Versatile sensing matrixes	102:127	Versatile sensing matrixes are essential for the development of enzyme-immobilized optical biosensors.
34340308	2	14	from	detection	305:313	arg1	sweat					371:375	artificial sweat	360:375	artificial sweat	360:375	A novel three-dimensional titanium dioxide nanotubes/alginate hydrogel scaffold is proposed for the detection of sweat biomarkers, lactate, and glucose in artificial sweat.
34340308	8	15	theme	nanotubes/alginate	1546:1563	arg1	scaffolds					1574:1582	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds	1382:1582	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds	1382:1582	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds provide a prospective opportunity for integration into wearable devices.
34340308	5	16	theme	synthesized	801:811	arg1	dioxide					822:828	the synthesized titanium dioxide	797:828	the synthesized titanium dioxide	797:828	The superhydrophilicity and the capillarity of the synthesized titanium dioxide nanotubes, when incorporated into the alginate matrix, facilitate the rapid transfer of the artificial sweat components throughout the sensor scaffold, decreasing the detection times.
34340308	5	17	theme	components	939:948	arg1	transfer					906:913	the rapid transfer	896:913	the rapid transfer of the artificial sweat components	896:948	The superhydrophilicity and the capillarity of the synthesized titanium dioxide nanotubes, when incorporated into the alginate matrix, facilitate the rapid transfer of the artificial sweat components throughout the sensor scaffold, decreasing the detection times.
34340308	6	18	theme	cellulose	1057:1065	arg1	paper					1067:1071	a cellulose paper	1055:1071	a cellulose paper to demonstrate the adaptability of the material to other matrixes	1055:1137	Moreover, the scaffold was integrated on a cellulose paper to demonstrate the adaptability of the material to other matrixes, obtaining fast and homogeneous colorimetric detection of lactate and glucose in the paper substrate when image analysis was performed.
34340308	8	19	theme	biocompatibility	1386:1401	arg1	scaffolds					1574:1582	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds	1382:1582	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds	1382:1582	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds provide a prospective opportunity for integration into wearable devices.
34340308	0	20	theme	Biomarkers	69:78	arg1	Sensing					52:58	Rapid Sensing	46:58	Rapid Sensing of Sweat Biomarkers: Lactate and Glucose	46:99	TiO2 Nanotubes Alginate Hydrogel Scaffold for Rapid Sensing of Sweat Biomarkers: Lactate and Glucose.
34340308	3	21	dep	introduced	437:446	arg1	followed					482:489	followed	482:489	followed by cross-linking nanocomposite with dicationic calcium ions to fabricate the scaffold platform	482:584	Hydrothermally synthesized titanium dioxide nanotubes were introduced to the alginate polymeric matrix, followed by cross-linking nanocomposite with dicationic calcium ions to fabricate the scaffold platform.
34340308	8	22	theme	immobilization	1418:1431	arg1	scaffolds					1574:1582	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds	1382:1582	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds	1382:1582	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds provide a prospective opportunity for integration into wearable devices.
34340308	6	23	theme	other	1124:1128	arg1	matrixes					1130:1137	other matrixes	1124:1137	other matrixes	1124:1137	Moreover, the scaffold was integrated on a cellulose paper to demonstrate the adaptability of the material to other matrixes, obtaining fast and homogeneous colorimetric detection of lactate and glucose in the paper substrate when image analysis was performed.
34340308	8	24	theme	wearable	1639:1646	arg1	devices					1648:1654	wearable devices	1639:1654	wearable devices	1639:1654	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds provide a prospective opportunity for integration into wearable devices.
34340308	8	25	theme	biological	1436:1445	arg1	assays					1468:1473	biological enzymes/colorimetric assays	1436:1473	biological enzymes/colorimetric assays	1436:1473	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds provide a prospective opportunity for integration into wearable devices.
34340308	3	26	theme	polymeric	464:472	arg1	matrix					474:479	the alginate polymeric matrix	451:479	the alginate polymeric matrix	451:479	Hydrothermally synthesized titanium dioxide nanotubes were introduced to the alginate polymeric matrix, followed by cross-linking nanocomposite with dicationic calcium ions to fabricate the scaffold platform.
34340308	0	27	dep	Biomarkers	69:78	arg1	Biomarkers					69:78	Sweat Biomarkers	63:78	Sweat Biomarkers: Lactate and Glucose	63:99	TiO2 Nanotubes Alginate Hydrogel Scaffold for Rapid Sensing of Sweat Biomarkers: Lactate and Glucose.
34340308	0	27	dep	Biomarkers	69:78	arg1	Glucose					93:99	Glucose	93:99	Glucose	93:99	TiO2 Nanotubes Alginate Hydrogel Scaffold for Rapid Sensing of Sweat Biomarkers: Lactate and Glucose.
34340308	0	27	dep	Biomarkers	69:78	arg1	Lactate					81:87	Lactate	81:87	Lactate	81:87	TiO2 Nanotubes Alginate Hydrogel Scaffold for Rapid Sensing of Sweat Biomarkers: Lactate and Glucose.
34340308	6	28	theme	material	1112:1119	arg1	adaptability					1092:1103	the adaptability	1088:1103	the adaptability of the material to other matrixes	1088:1137	Moreover, the scaffold was integrated on a cellulose paper to demonstrate the adaptability of the material to other matrixes, obtaining fast and homogeneous colorimetric detection of lactate and glucose in the paper substrate when image analysis was performed.
34340308	3	29	theme	titanium	405:412	arg1	dioxide					414:420	titanium dioxide	405:420	Hydrothermally synthesized titanium dioxide nanotubes	378:430	Hydrothermally synthesized titanium dioxide nanotubes were introduced to the alginate polymeric matrix, followed by cross-linking nanocomposite with dicationic calcium ions to fabricate the scaffold platform.
34340308	8	30	theme	assays	1468:1473	arg1	behavior					1513:1520	the quick optical signal readout behavior	1480:1520	the quick optical signal readout behavior of the titanium dioxide	1480:1544	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds provide a prospective opportunity for integration into wearable devices.
34340308	8	30	theme	assays	1468:1473	arg1	immobilization					1418:1431	the efficient immobilization	1404:1431	the efficient immobilization of biological enzymes/colorimetric assays	1404:1473	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds provide a prospective opportunity for integration into wearable devices.
34340308	8	30	theme	assays	1468:1473	arg1	biocompatibility					1386:1401	biocompatibility	1386:1401	biocompatibility	1386:1401	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds provide a prospective opportunity for integration into wearable devices.
34340308	0	31	theme	Alginate	15:22	arg1	Scaffold					33:40	Alginate Hydrogel Scaffold	15:40	Alginate Hydrogel Scaffold for Rapid Sensing of Sweat Biomarkers: Lactate and Glucose	15:99	TiO2 Nanotubes Alginate Hydrogel Scaffold for Rapid Sensing of Sweat Biomarkers: Lactate and Glucose.
34340308	7	32	theme	sensor	1366:1371	arg1	devices					1373:1379	paper-based sensor devices	1354:1379	paper-based sensor devices	1354:1379	The properties of this new composite provide new avenues in the development of paper-based sensor devices.
34340308	4	33	theme	color	622:626	arg1	signal					636:641	blue color optical signal	617:641	blue color optical signal	617:641	Rapid colorimetric detection (blue color optical signal) was carried out for both lactate and glucose biomarkers in artificial sweat at 4 and 6 min, respectively.
34340308	4	33	theme	color	622:626	arg1	detection					606:614	Rapid colorimetric detection	587:614	Rapid colorimetric detection (blue color optical signal)	587:642	Rapid colorimetric detection (blue color optical signal) was carried out for both lactate and glucose biomarkers in artificial sweat at 4 and 6 min, respectively.
34340308	8	34	theme	quick	1484:1488	arg1	behavior					1513:1520	the quick optical signal readout behavior	1480:1520	the quick optical signal readout behavior of the titanium dioxide	1480:1544	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds provide a prospective opportunity for integration into wearable devices.
34340308	2	35	theme	artificial	360:369	arg1	sweat					371:375	artificial sweat	360:375	artificial sweat	360:375	A novel three-dimensional titanium dioxide nanotubes/alginate hydrogel scaffold is proposed for the detection of sweat biomarkers, lactate, and glucose in artificial sweat.
34340308	0	36	theme	Rapid	46:50	arg1	Sensing					52:58	Rapid Sensing	46:58	Rapid Sensing of Sweat Biomarkers: Lactate and Glucose	46:99	TiO2 Nanotubes Alginate Hydrogel Scaffold for Rapid Sensing of Sweat Biomarkers: Lactate and Glucose.
34340308	4	37	theme	colorimetric	593:604	arg1	signal					636:641	blue color optical signal	617:641	blue color optical signal	617:641	Rapid colorimetric detection (blue color optical signal) was carried out for both lactate and glucose biomarkers in artificial sweat at 4 and 6 min, respectively.
34340308	4	37	theme	colorimetric	593:604	arg1	detection					606:614	Rapid colorimetric detection	587:614	Rapid colorimetric detection (blue color optical signal)	587:642	Rapid colorimetric detection (blue color optical signal) was carried out for both lactate and glucose biomarkers in artificial sweat at 4 and 6 min, respectively.
34340308	2	38	theme	novel	207:211	arg1	dioxide					240:246	A novel three-dimensional titanium dioxide	205:246	A novel three-dimensional titanium dioxide nanotubes/alginate hydrogel scaffold	205:283	A novel three-dimensional titanium dioxide nanotubes/alginate hydrogel scaffold is proposed for the detection of sweat biomarkers, lactate, and glucose in artificial sweat.
34340308	8	39	theme	signal	1498:1503	arg1	behavior					1513:1520	the quick optical signal readout behavior	1480:1520	the quick optical signal readout behavior of the titanium dioxide	1480:1544	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds provide a prospective opportunity for integration into wearable devices.
34340308	6	40	from	detection	1184:1192	arg1	substrate					1230:1238	the paper substrate	1220:1238	the paper substrate	1220:1238	Moreover, the scaffold was integrated on a cellulose paper to demonstrate the adaptability of the material to other matrixes, obtaining fast and homogeneous colorimetric detection of lactate and glucose in the paper substrate when image analysis was performed.
34340308	3	41	theme	dicationic	527:536	arg1	ions					546:549	dicationic calcium ions	527:549	dicationic calcium ions	527:549	Hydrothermally synthesized titanium dioxide nanotubes were introduced to the alginate polymeric matrix, followed by cross-linking nanocomposite with dicationic calcium ions to fabricate the scaffold platform.
34340308	6	42	theme	homogeneous	1159:1169	arg1	detection					1184:1192	fast and homogeneous colorimetric detection	1150:1192	fast and homogeneous colorimetric detection of lactate and glucose in the paper substrate	1150:1238	Moreover, the scaffold was integrated on a cellulose paper to demonstrate the adaptability of the material to other matrixes, obtaining fast and homogeneous colorimetric detection of lactate and glucose in the paper substrate when image analysis was performed.
34340308	8	43	theme	behavior	1513:1520	arg1	scaffolds					1574:1582	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds	1382:1582	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds	1382:1582	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds provide a prospective opportunity for integration into wearable devices.
34340308	3	44	theme	scaffold	568:575	arg1	platform					577:584	the scaffold platform	564:584	the scaffold platform	564:584	Hydrothermally synthesized titanium dioxide nanotubes were introduced to the alginate polymeric matrix, followed by cross-linking nanocomposite with dicationic calcium ions to fabricate the scaffold platform.
34340308	6	45	theme	fast	1150:1153	arg1	detection					1184:1192	fast and homogeneous colorimetric detection	1150:1192	fast and homogeneous colorimetric detection of lactate and glucose in the paper substrate	1150:1238	Moreover, the scaffold was integrated on a cellulose paper to demonstrate the adaptability of the material to other matrixes, obtaining fast and homogeneous colorimetric detection of lactate and glucose in the paper substrate when image analysis was performed.
34340308	4	46	from	min	731:733	arg1	sweat					714:718	artificial sweat	703:718	artificial sweat at 4 and 6 min	703:733	Rapid colorimetric detection (blue color optical signal) was carried out for both lactate and glucose biomarkers in artificial sweat at 4 and 6 min, respectively.
34340308	7	47	theme	new	1298:1300	arg1	composite					1302:1310	this new composite	1293:1310	this new composite	1293:1310	The properties of this new composite provide new avenues in the development of paper-based sensor devices.
34340308	2	48	theme	sweat	318:322	arg1	lactate					336:342	lactate	336:342	lactate	336:342	A novel three-dimensional titanium dioxide nanotubes/alginate hydrogel scaffold is proposed for the detection of sweat biomarkers, lactate, and glucose in artificial sweat.
34340308	2	48	theme	sweat	318:322	arg1	glucose					349:355	glucose	349:355	glucose	349:355	A novel three-dimensional titanium dioxide nanotubes/alginate hydrogel scaffold is proposed for the detection of sweat biomarkers, lactate, and glucose in artificial sweat.
34340308	2	48	theme	sweat	318:322	arg1	biomarkers					324:333	sweat biomarkers	318:333	sweat biomarkers	318:333	A novel three-dimensional titanium dioxide nanotubes/alginate hydrogel scaffold is proposed for the detection of sweat biomarkers, lactate, and glucose in artificial sweat.
34340308	4	49	theme	artificial	703:712	arg1	sweat					714:718	artificial sweat	703:718	artificial sweat at 4 and 6 min	703:733	Rapid colorimetric detection (blue color optical signal) was carried out for both lactate and glucose biomarkers in artificial sweat at 4 and 6 min, respectively.
34340308	5	50	theme	dioxide	822:828	arg1	capillarity					782:792	the capillarity	778:792	the capillarity of the synthesized titanium dioxide	778:828	The superhydrophilicity and the capillarity of the synthesized titanium dioxide nanotubes, when incorporated into the alginate matrix, facilitate the rapid transfer of the artificial sweat components throughout the sensor scaffold, decreasing the detection times.
34340308	5	50	theme	dioxide	822:828	arg1	superhydrophilicity					754:772	superhydrophilicity	754:772	superhydrophilicity	754:772	The superhydrophilicity and the capillarity of the synthesized titanium dioxide nanotubes, when incorporated into the alginate matrix, facilitate the rapid transfer of the artificial sweat components throughout the sensor scaffold, decreasing the detection times.
34340308	1	51	theme	optical	185:191	arg1	biosensors					193:202	enzyme-immobilized optical biosensors	166:202	enzyme-immobilized optical biosensors	166:202	Versatile sensing matrixes are essential for the development of enzyme-immobilized optical biosensors.
34340308	6	52	theme	glucose	1209:1215	arg1	detection					1184:1192	fast and homogeneous colorimetric detection	1150:1192	fast and homogeneous colorimetric detection of lactate and glucose in the paper substrate	1150:1238	Moreover, the scaffold was integrated on a cellulose paper to demonstrate the adaptability of the material to other matrixes, obtaining fast and homogeneous colorimetric detection of lactate and glucose in the paper substrate when image analysis was performed.
34340308	6	53	theme	lactate	1197:1203	arg1	detection					1184:1192	fast and homogeneous colorimetric detection	1150:1192	fast and homogeneous colorimetric detection of lactate and glucose in the paper substrate	1150:1238	Moreover, the scaffold was integrated on a cellulose paper to demonstrate the adaptability of the material to other matrixes, obtaining fast and homogeneous colorimetric detection of lactate and glucose in the paper substrate when image analysis was performed.
34340308	3	54	with	nanocomposite	508:520	arg1	ions					546:549	dicationic calcium ions	527:549	dicationic calcium ions	527:549	Hydrothermally synthesized titanium dioxide nanotubes were introduced to the alginate polymeric matrix, followed by cross-linking nanocomposite with dicationic calcium ions to fabricate the scaffold platform.
34340308	2	55	theme	hydrogel	267:274	arg1	scaffold					276:283	A novel three-dimensional titanium dioxide nanotubes/alginate hydrogel scaffold	205:283	A novel three-dimensional titanium dioxide nanotubes/alginate hydrogel scaffold	205:283	A novel three-dimensional titanium dioxide nanotubes/alginate hydrogel scaffold is proposed for the detection of sweat biomarkers, lactate, and glucose in artificial sweat.
34340308	5	56	theme	artificial	922:931	arg1	components					939:948	the artificial sweat components	918:948	the artificial sweat components	918:948	The superhydrophilicity and the capillarity of the synthesized titanium dioxide nanotubes, when incorporated into the alginate matrix, facilitate the rapid transfer of the artificial sweat components throughout the sensor scaffold, decreasing the detection times.
34340308	8	57	theme	dioxide	1538:1544	arg1	behavior					1513:1520	the quick optical signal readout behavior	1480:1520	the quick optical signal readout behavior of the titanium dioxide	1480:1544	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds provide a prospective opportunity for integration into wearable devices.
34340308	8	57	theme	dioxide	1538:1544	arg1	immobilization					1418:1431	the efficient immobilization	1404:1431	the efficient immobilization of biological enzymes/colorimetric assays	1404:1473	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds provide a prospective opportunity for integration into wearable devices.
34340308	8	57	theme	dioxide	1538:1544	arg1	biocompatibility					1386:1401	biocompatibility	1386:1401	biocompatibility	1386:1401	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds provide a prospective opportunity for integration into wearable devices.
34340308	2	58	theme	dioxide	240:246	arg1	scaffold					276:283	A novel three-dimensional titanium dioxide nanotubes/alginate hydrogel scaffold	205:283	A novel three-dimensional titanium dioxide nanotubes/alginate hydrogel scaffold	205:283	A novel three-dimensional titanium dioxide nanotubes/alginate hydrogel scaffold is proposed for the detection of sweat biomarkers, lactate, and glucose in artificial sweat.
34340308	1	59	theme	Versatile	102:110	arg1	matrixes					120:127	Versatile sensing matrixes	102:127	Versatile sensing matrixes	102:127	Versatile sensing matrixes are essential for the development of enzyme-immobilized optical biosensors.
34340308	8	60	theme	hydrogel	1565:1572	arg1	scaffolds					1574:1582	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds	1382:1582	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds	1382:1582	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds provide a prospective opportunity for integration into wearable devices.
34340308	6	61	theme	colorimetric	1171:1182	arg1	detection					1184:1192	fast and homogeneous colorimetric detection	1150:1192	fast and homogeneous colorimetric detection of lactate and glucose in the paper substrate	1150:1238	Moreover, the scaffold was integrated on a cellulose paper to demonstrate the adaptability of the material to other matrixes, obtaining fast and homogeneous colorimetric detection of lactate and glucose in the paper substrate when image analysis was performed.
34340308	0	62	theme	Sweat	63:67	arg1	Biomarkers					69:78	Sweat Biomarkers	63:78	Sweat Biomarkers: Lactate and Glucose	63:99	TiO2 Nanotubes Alginate Hydrogel Scaffold for Rapid Sensing of Sweat Biomarkers: Lactate and Glucose.
34340308	0	62	theme	Sweat	63:67	arg1	Glucose					93:99	Glucose	93:99	Glucose	93:99	TiO2 Nanotubes Alginate Hydrogel Scaffold for Rapid Sensing of Sweat Biomarkers: Lactate and Glucose.
34340308	0	62	theme	Sweat	63:67	arg1	Lactate					81:87	Lactate	81:87	Lactate	81:87	TiO2 Nanotubes Alginate Hydrogel Scaffold for Rapid Sensing of Sweat Biomarkers: Lactate and Glucose.
34340308	5	63	theme	sweat	933:937	arg1	components					939:948	the artificial sweat components	918:948	the artificial sweat components	918:948	The superhydrophilicity and the capillarity of the synthesized titanium dioxide nanotubes, when incorporated into the alginate matrix, facilitate the rapid transfer of the artificial sweat components throughout the sensor scaffold, decreasing the detection times.
34340308	8	64	theme	prospective	1594:1604	arg1	opportunity					1606:1616	a prospective opportunity	1592:1616	a prospective opportunity for integration into wearable devices	1592:1654	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds provide a prospective opportunity for integration into wearable devices.
34340308	6	65	theme	image	1245:1249	arg1	analysis					1251:1258	image analysis	1245:1258	image analysis	1245:1258	Moreover, the scaffold was integrated on a cellulose paper to demonstrate the adaptability of the material to other matrixes, obtaining fast and homogeneous colorimetric detection of lactate and glucose in the paper substrate when image analysis was performed.
34340308	5	66	theme	titanium	813:820	arg1	dioxide					822:828	the synthesized titanium dioxide	797:828	the synthesized titanium dioxide	797:828	The superhydrophilicity and the capillarity of the synthesized titanium dioxide nanotubes, when incorporated into the alginate matrix, facilitate the rapid transfer of the artificial sweat components throughout the sensor scaffold, decreasing the detection times.
34340308	3	67	theme	synthesized	393:403	arg1	nanotubes					422:430	Hydrothermally synthesized titanium dioxide nanotubes	378:430	Hydrothermally synthesized titanium dioxide nanotubes	378:430	Hydrothermally synthesized titanium dioxide nanotubes were introduced to the alginate polymeric matrix, followed by cross-linking nanocomposite with dicationic calcium ions to fabricate the scaffold platform.
34340308	5	68	theme	capillarity	782:792	arg1	nanotubes					830:838	The superhydrophilicity and the capillarity of the synthesized titanium dioxide nanotubes	750:838	The superhydrophilicity and the capillarity of the synthesized titanium dioxide nanotubes	750:838	The superhydrophilicity and the capillarity of the synthesized titanium dioxide nanotubes, when incorporated into the alginate matrix, facilitate the rapid transfer of the artificial sweat components throughout the sensor scaffold, decreasing the detection times.
34340308	8	69	theme	efficient	1408:1416	arg1	immobilization					1418:1431	the efficient immobilization	1404:1431	the efficient immobilization of biological enzymes/colorimetric assays	1404:1473	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds provide a prospective opportunity for integration into wearable devices.
34340308	7	70	theme	devices	1373:1379	arg1	development					1339:1349	the development	1335:1349	the development of paper-based sensor devices	1335:1379	The properties of this new composite provide new avenues in the development of paper-based sensor devices.
34340308	4	71	theme	blue	617:620	arg1	signal					636:641	blue color optical signal	617:641	blue color optical signal	617:641	Rapid colorimetric detection (blue color optical signal) was carried out for both lactate and glucose biomarkers in artificial sweat at 4 and 6 min, respectively.
34340308	4	71	theme	blue	617:620	arg1	detection					606:614	Rapid colorimetric detection	587:614	Rapid colorimetric detection (blue color optical signal)	587:642	Rapid colorimetric detection (blue color optical signal) was carried out for both lactate and glucose biomarkers in artificial sweat at 4 and 6 min, respectively.
34340308	0	72	theme	Hydrogel	24:31	arg1	Scaffold					33:40	Alginate Hydrogel Scaffold	15:40	Alginate Hydrogel Scaffold for Rapid Sensing of Sweat Biomarkers: Lactate and Glucose	15:99	TiO2 Nanotubes Alginate Hydrogel Scaffold for Rapid Sensing of Sweat Biomarkers: Lactate and Glucose.
34340308	7	73	theme	paper-based	1354:1364	arg1	devices					1373:1379	paper-based sensor devices	1354:1379	paper-based sensor devices	1354:1379	The properties of this new composite provide new avenues in the development of paper-based sensor devices.
34340308	3	74	theme	dioxide	414:420	arg1	nanotubes					422:430	Hydrothermally synthesized titanium dioxide nanotubes	378:430	Hydrothermally synthesized titanium dioxide nanotubes	378:430	Hydrothermally synthesized titanium dioxide nanotubes were introduced to the alginate polymeric matrix, followed by cross-linking nanocomposite with dicationic calcium ions to fabricate the scaffold platform.
34340308	4	75	theme	optical	628:634	arg1	signal					636:641	blue color optical signal	617:641	blue color optical signal	617:641	Rapid colorimetric detection (blue color optical signal) was carried out for both lactate and glucose biomarkers in artificial sweat at 4 and 6 min, respectively.
34340308	4	75	theme	optical	628:634	arg1	detection					606:614	Rapid colorimetric detection	587:614	Rapid colorimetric detection (blue color optical signal)	587:642	Rapid colorimetric detection (blue color optical signal) was carried out for both lactate and glucose biomarkers in artificial sweat at 4 and 6 min, respectively.
34340308	8	76	theme	enzymes/colorimetric	1447:1466	arg1	assays					1468:1473	biological enzymes/colorimetric assays	1436:1473	biological enzymes/colorimetric assays	1436:1473	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds provide a prospective opportunity for integration into wearable devices.
34340308	3	77	theme	alginate	455:462	arg1	matrix					474:479	the alginate polymeric matrix	451:479	the alginate polymeric matrix	451:479	Hydrothermally synthesized titanium dioxide nanotubes were introduced to the alginate polymeric matrix, followed by cross-linking nanocomposite with dicationic calcium ions to fabricate the scaffold platform.
34340308	4	78	theme	Rapid	587:591	arg1	signal					636:641	blue color optical signal	617:641	blue color optical signal	617:641	Rapid colorimetric detection (blue color optical signal) was carried out for both lactate and glucose biomarkers in artificial sweat at 4 and 6 min, respectively.
34340308	4	78	theme	Rapid	587:591	arg1	detection					606:614	Rapid colorimetric detection	587:614	Rapid colorimetric detection (blue color optical signal)	587:642	Rapid colorimetric detection (blue color optical signal) was carried out for both lactate and glucose biomarkers in artificial sweat at 4 and 6 min, respectively.
34340308	2	79	theme	three-dimensional	213:229	arg1	dioxide					240:246	A novel three-dimensional titanium dioxide	205:246	A novel three-dimensional titanium dioxide nanotubes/alginate hydrogel scaffold	205:283	A novel three-dimensional titanium dioxide nanotubes/alginate hydrogel scaffold is proposed for the detection of sweat biomarkers, lactate, and glucose in artificial sweat.
34340308	2	80	theme	nanotubes/alginate	248:265	arg1	scaffold					276:283	A novel three-dimensional titanium dioxide nanotubes/alginate hydrogel scaffold	205:283	A novel three-dimensional titanium dioxide nanotubes/alginate hydrogel scaffold	205:283	A novel three-dimensional titanium dioxide nanotubes/alginate hydrogel scaffold is proposed for the detection of sweat biomarkers, lactate, and glucose in artificial sweat.
34340308	7	81	theme	new	1320:1322	arg1	avenues					1324:1330	new avenues	1320:1330	new avenues in the development of paper-based sensor devices	1320:1379	The properties of this new composite provide new avenues in the development of paper-based sensor devices.
34340308	8	82	theme	optical	1490:1496	arg1	behavior					1513:1520	the quick optical signal readout behavior	1480:1520	the quick optical signal readout behavior of the titanium dioxide	1480:1544	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds provide a prospective opportunity for integration into wearable devices.
34340308	3	83	theme	calcium	538:544	arg1	ions					546:549	dicationic calcium ions	527:549	dicationic calcium ions	527:549	Hydrothermally synthesized titanium dioxide nanotubes were introduced to the alginate polymeric matrix, followed by cross-linking nanocomposite with dicationic calcium ions to fabricate the scaffold platform.
34340308	7	84	theme	composite	1302:1310	arg1	properties					1279:1288	The properties	1275:1288	The properties of this new composite	1275:1310	The properties of this new composite provide new avenues in the development of paper-based sensor devices.
34340308	8	85	theme	readout	1505:1511	arg1	behavior					1513:1520	the quick optical signal readout behavior	1480:1520	the quick optical signal readout behavior of the titanium dioxide	1480:1544	The biocompatibility, the efficient immobilization of biological enzymes/colorimetric assays, and the quick optical signal readout behavior of the titanium dioxide nanotubes/alginate hydrogel scaffolds provide a prospective opportunity for integration into wearable devices.
32282427	13	0	theme	U.S.	2315:2318	arg1	hospitals					2320:2328	U.S. hospitals	2315:2328	U.S. hospitals	2315:2328	CONCLUSIONS Among a generalizable cohort of adult patients undergoing inpatient surgery at U.S. hospitals, the use of sugammadex was associated with a clinically and statistically significant lower incidence of major pulmonary complications.
32282427	3	1	theme	multicenter	557:567	arg1	study					598:602	a multicenter observational matched-cohort study	555:602	a multicenter observational matched-cohort study of surgical cases between January 2014 and August 2018	555:657	METHODS Twelve U.S. Multicenter Perioperative Outcomes Group hospitals were included in a multicenter observational matched-cohort study of surgical cases between January 2014 and August 2018.
32282427	5	2	dep	age	924:926	arg1	type					972:975	type	972:975	type	972:975	Exact matching criteria included institution, sex, age, comorbidities, obesity, surgical procedure type, and neuromuscular blockade agent (rocuronium vs. vecuronium).
32282427	4	3	theme	Adult	660:664	arg1	patients					666:673	Adult patients	660:673	Adult patients undergoing elective inpatient noncardiac surgical procedures with general anesthesia and endotracheal intubation receiving a nondepolarizing neuromuscular blockade agent and reversal	660:856	Adult patients undergoing elective inpatient noncardiac surgical procedures with general anesthesia and endotracheal intubation receiving a nondepolarizing neuromuscular blockade agent and reversal were included.
32282427	10	4	theme	%	1703:1703	arg1	sugammadex					1705:1714	3.5% sugammadex	1700:1714	3.5% sugammadex	1700:1714	Out of 45,712 patients studied, 1,892 (4.1%) were diagnosed with the composite primary outcome (3.5% sugammadex vs. 4.8% neostigmine).
32282427	11	5	theme	patients	1761:1768	arg1	total					1741:1745	A total	1739:1745	A total of 796 (1.7%) patients	1739:1768	A total of 796 (1.7%) patients had pneumonia (1.3% vs. 2.2%), and 582 (1.3%) respiratory failure (0.8% vs. 1.7%).
32282427	3	6	theme	cases	616:620	arg1	study					598:602	a multicenter observational matched-cohort study	555:602	a multicenter observational matched-cohort study of surgical cases between January 2014 and August 2018	555:657	METHODS Twelve U.S. Multicenter Perioperative Outcomes Group hospitals were included in a multicenter observational matched-cohort study of surgical cases between January 2014 and August 2018.
32282427	4	7	theme	endotracheal	764:775	arg1	intubation					777:786	endotracheal intubation	764:786	endotracheal intubation	764:786	Adult patients undergoing elective inpatient noncardiac surgical procedures with general anesthesia and endotracheal intubation receiving a nondepolarizing neuromuscular blockade agent and reversal were included.
32282427	10	8	theme	%	1723:1723	arg1	neostigmine					1725:1735	4.8% neostigmine	1720:1735	4.8% neostigmine	1720:1735	Out of 45,712 patients studied, 1,892 (4.1%) were diagnosed with the composite primary outcome (3.5% sugammadex vs. 4.8% neostigmine).
32282427	2	9	theme	blockade	345:352	arg1	reversal					354:361	neuromuscular blockade reversal	331:361	neuromuscular blockade reversal (neostigmine vs. sugammadex)	331:390	The authors hypothesized that the choice of neuromuscular blockade reversal (neostigmine vs. sugammadex) may be associated with a lower incidence of major pulmonary complications.
32282427	1	10	theme	patients	195:202	arg1	percent					178:184	BACKGROUND Five percent	162:184	BACKGROUND Five percent of adult patients undergoing noncardiac inpatient surgery	162:242	BACKGROUND Five percent of adult patients undergoing noncardiac inpatient surgery experience a major pulmonary complication.
32282427	4	11	theme	surgical	716:723	arg1	procedures					725:734	elective inpatient noncardiac surgical procedures	686:734	elective inpatient noncardiac surgical procedures	686:734	Adult patients undergoing elective inpatient noncardiac surgical procedures with general anesthesia and endotracheal intubation receiving a nondepolarizing neuromuscular blockade agent and reversal were included.
32282427	7	12	theme	pulmonary	1202:1210	arg1	complications					1212:1224	major postoperative pulmonary complications	1182:1224	major postoperative pulmonary complications	1182:1224	The composite primary outcome was major postoperative pulmonary complications, defined as pneumonia, respiratory failure, or other pulmonary complications (including pneumonitis; pulmonary congestion; iatrogenic pulmonary embolism, infarction, or pneumothorax).
32282427	7	12	theme	pulmonary	1202:1210	arg1	outcome					1170:1176	The composite primary outcome	1148:1176	The composite primary outcome	1148:1176	The composite primary outcome was major postoperative pulmonary complications, defined as pneumonia, respiratory failure, or other pulmonary complications (including pneumonitis; pulmonary congestion; iatrogenic pulmonary embolism, infarction, or pneumothorax).
32282427	1	13	theme	noncardiac	215:224	arg1	surgery					236:242	noncardiac inpatient surgery	215:242	noncardiac inpatient surgery	215:242	BACKGROUND Five percent of adult patients undergoing noncardiac inpatient surgery experience a major pulmonary complication.
32282427	12	14	theme	complications	1958:1970	arg1	risk					1940:1943	risk	1940:1943	risk of pulmonary complications	1940:1970	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	7	15	theme	major	1182:1186	arg1	complications					1212:1224	major postoperative pulmonary complications	1182:1224	major postoperative pulmonary complications	1182:1224	The composite primary outcome was major postoperative pulmonary complications, defined as pneumonia, respiratory failure, or other pulmonary complications (including pneumonitis; pulmonary congestion; iatrogenic pulmonary embolism, infarction, or pneumothorax).
32282427	7	15	theme	major	1182:1186	arg1	outcome					1170:1176	The composite primary outcome	1148:1176	The composite primary outcome	1148:1176	The composite primary outcome was major postoperative pulmonary complications, defined as pneumonia, respiratory failure, or other pulmonary complications (including pneumonitis; pulmonary congestion; iatrogenic pulmonary embolism, infarction, or pneumothorax).
32282427	12	16	theme	adjusted	1973:1980	arg1	ratio					1987:1991	adjusted odds ratio	1973:1991	adjusted odds ratio	1973:1991	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	6	17	theme	Other	1040:1044	arg1	factors					1078:1084	Other preoperative and intraoperative factors	1040:1084	Other preoperative and intraoperative factors	1040:1084	Other preoperative and intraoperative factors were compared and adjusted in the case of residual imbalance.
32282427	2	18	dep	reversal	354:361	arg1	sugammadex					380:389	sugammadex	380:389	sugammadex	380:389	The authors hypothesized that the choice of neuromuscular blockade reversal (neostigmine vs. sugammadex) may be associated with a lower incidence of major pulmonary complications.
32282427	2	18	dep	reversal	354:361	arg1	neostigmine					364:374	neostigmine	364:374	neostigmine	364:374	The authors hypothesized that the choice of neuromuscular blockade reversal (neostigmine vs. sugammadex) may be associated with a lower incidence of major pulmonary complications.
32282427	13	19	theme	adult	2268:2272	arg1	patients					2274:2281	adult patients	2268:2281	adult patients undergoing inpatient surgery at U.S. hospitals	2268:2328	CONCLUSIONS Among a generalizable cohort of adult patients undergoing inpatient surgery at U.S. hospitals, the use of sugammadex was associated with a clinically and statistically significant lower incidence of major pulmonary complications.
32282427	11	20	theme	582	1805:1807	arg1	failure					1828:1834	582 (1.3%) respiratory failure	1805:1834	582 (1.3%) respiratory failure (0.8% vs. 1.7%)	1805:1850	A total of 796 (1.7%) patients had pneumonia (1.3% vs. 2.2%), and 582 (1.3%) respiratory failure (0.8% vs. 1.7%).
32282427	7	21	theme	composite	1152:1160	arg1	complications					1212:1224	major postoperative pulmonary complications	1182:1224	major postoperative pulmonary complications	1182:1224	The composite primary outcome was major postoperative pulmonary complications, defined as pneumonia, respiratory failure, or other pulmonary complications (including pneumonitis; pulmonary congestion; iatrogenic pulmonary embolism, infarction, or pneumothorax).
32282427	7	21	theme	composite	1152:1160	arg1	outcome					1170:1176	The composite primary outcome	1148:1176	The composite primary outcome	1148:1176	The composite primary outcome was major postoperative pulmonary complications, defined as pneumonia, respiratory failure, or other pulmonary complications (including pneumonitis; pulmonary congestion; iatrogenic pulmonary embolism, infarction, or pneumothorax).
32282427	6	22	theme	imbalance	1137:1145	arg1	case					1120:1123	the case	1116:1123	the case of residual imbalance	1116:1145	Other preoperative and intraoperative factors were compared and adjusted in the case of residual imbalance.
32282427	8	23	theme	pneumonia	1458:1466	arg1	components					1444:1453	the components	1440:1453	the components of pneumonia and respiratory failure	1440:1490	Secondary outcomes focused on the components of pneumonia and respiratory failure.
32282427	12	24	dep	0.62	2097:2100	arg1	to					2094:2095	to	2094:2095	to	2094:2095	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	5	25	theme	matching	879:886	arg1	criteria					888:895	Exact matching criteria	873:895	Exact matching criteria	873:895	Exact matching criteria included institution, sex, age, comorbidities, obesity, surgical procedure type, and neuromuscular blockade agent (rocuronium vs. vecuronium).
32282427	11	26	dep	failure	1828:1834	arg1	%					1840:1840	0.8%	1837:1840	0.8%	1837:1840	A total of 796 (1.7%) patients had pneumonia (1.3% vs. 2.2%), and 582 (1.3%) respiratory failure (0.8% vs. 1.7%).
32282427	11	26	dep	failure	1828:1834	arg1	%					1849:1849	1.7%	1846:1849	1.7%	1846:1849	A total of 796 (1.7%) patients had pneumonia (1.3% vs. 2.2%), and 582 (1.3%) respiratory failure (0.8% vs. 1.7%).
32282427	4	27	theme	nondepolarizing	800:814	arg1	blockade					830:837	a nondepolarizing neuromuscular blockade agent and reversal	798:856	a nondepolarizing neuromuscular blockade agent and reversal	798:856	Adult patients undergoing elective inpatient noncardiac surgical procedures with general anesthesia and endotracheal intubation receiving a nondepolarizing neuromuscular blockade agent and reversal were included.
32282427	0	28	theme	Pulmonary	87:95	arg1	Complications					97:109	Postoperative Pulmonary Complications	73:109	Postoperative Pulmonary Complications (STRONGER)	73:120	Sugammadex versus Neostigmine for Reversal of Neuromuscular Blockade and Postoperative Pulmonary Complications (STRONGER): A Multicenter Matched Cohort Analysis.
32282427	12	29	dep	risk	2120:2123	arg1	0.56					2192:2195	0.56	2192:2195	0.56	2192:2195	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	12	29	dep	risk	2120:2123	arg1	%					2178:2178	0.45; 95% CI	2170:2181	0.45; 95% CI	2170:2181	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	12	29	dep	risk	2120:2123	arg1	ratio					2163:2167	adjusted odds ratio	2149:2167	adjusted odds ratio	2149:2167	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	13	30	theme	inpatient	2294:2302	arg1	surgery					2304:2310	inpatient surgery	2294:2310	inpatient surgery	2294:2310	CONCLUSIONS Among a generalizable cohort of adult patients undergoing inpatient surgery at U.S. hospitals, the use of sugammadex was associated with a clinically and statistically significant lower incidence of major pulmonary complications.
32282427	3	31	theme	Group	522:526	arg1	hospitals					528:536	Group hospitals	522:536	Group hospitals	522:536	METHODS Twelve U.S. Multicenter Perioperative Outcomes Group hospitals were included in a multicenter observational matched-cohort study of surgical cases between January 2014 and August 2018.
32282427	12	32	theme	failure	2140:2146	arg1	risk					2120:2123	risk	2120:2123	risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56)	2120:2196	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	7	33	theme	pulmonary	1327:1335	arg1	congestion					1337:1346	pulmonary congestion	1327:1346	pulmonary congestion	1327:1346	The composite primary outcome was major postoperative pulmonary complications, defined as pneumonia, respiratory failure, or other pulmonary complications (including pneumonitis; pulmonary congestion; iatrogenic pulmonary embolism, infarction, or pneumothorax).
32282427	11	34	contain	had	1770:1772	arg2	pneumonia					1774:1782	pneumonia	1774:1782	pneumonia (1.3% vs. 2.2%)	1774:1798	A total of 796 (1.7%) patients had pneumonia (1.3% vs. 2.2%), and 582 (1.3%) respiratory failure (0.8% vs. 1.7%).
32282427	11	34	contain	had	1770:1772	arg2	failure					1828:1834	582 (1.3%) respiratory failure	1805:1834	582 (1.3%) respiratory failure (0.8% vs. 1.7%)	1805:1850	A total of 796 (1.7%) patients had pneumonia (1.3% vs. 2.2%), and 582 (1.3%) respiratory failure (0.8% vs. 1.7%).
32282427	11	34	contain	had	1770:1772	arg1	total					1741:1745	A total	1739:1745	A total of 796 (1.7%) patients	1739:1768	A total of 796 (1.7%) patients had pneumonia (1.3% vs. 2.2%), and 582 (1.3%) respiratory failure (0.8% vs. 1.7%).
32282427	13	35	dep	significant	2404:2414	arg1	lower					2416:2420	lower	2416:2420	lower	2416:2420	CONCLUSIONS Among a generalizable cohort of adult patients undergoing inpatient surgery at U.S. hospitals, the use of sugammadex was associated with a clinically and statistically significant lower incidence of major pulmonary complications.
32282427	12	36	theme	odds	2158:2161	arg1	0.56					2192:2195	0.56	2192:2195	0.56	2192:2195	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	12	36	theme	odds	2158:2161	arg1	%					2178:2178	0.45; 95% CI	2170:2181	0.45; 95% CI	2170:2181	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	12	36	theme	odds	2158:2161	arg1	ratio					2163:2167	adjusted odds ratio	2149:2167	adjusted odds ratio	2149:2167	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	12	37	dep	ratio	1987:1991	arg1	%					2002:2002	95% CI	2000:2005	95% CI	2000:2005	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	12	37	dep	ratio	1987:1991	arg1	0.77					2016:2019	0.77	2016:2019	0.77	2016:2019	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	12	37	dep	ratio	1987:1991	arg1	0.70					1994:1997	0.70	1994:1997	0.70	1994:1997	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	0	38	theme	Postoperative	73:85	arg1	Complications					97:109	Postoperative Pulmonary Complications	73:109	Postoperative Pulmonary Complications (STRONGER)	73:120	Sugammadex versus Neostigmine for Reversal of Neuromuscular Blockade and Postoperative Pulmonary Complications (STRONGER): A Multicenter Matched Cohort Analysis.
32282427	2	39	theme	complications	452:464	arg1	incidence					423:431	a lower incidence	415:431	a lower incidence of major pulmonary complications	415:464	The authors hypothesized that the choice of neuromuscular blockade reversal (neostigmine vs. sugammadex) may be associated with a lower incidence of major pulmonary complications.
32282427	13	40	theme	patients	2274:2281	arg1	cohort					2258:2263	a generalizable cohort	2242:2263	a generalizable cohort of adult patients undergoing inpatient surgery at U.S. hospitals	2242:2328	CONCLUSIONS Among a generalizable cohort of adult patients undergoing inpatient surgery at U.S. hospitals, the use of sugammadex was associated with a clinically and statistically significant lower incidence of major pulmonary complications.
32282427	13	40	theme	patients	2274:2281	arg1	use					2335:2337	the use	2331:2337	the use of sugammadex	2331:2351	CONCLUSIONS Among a generalizable cohort of adult patients undergoing inpatient surgery at U.S. hospitals, the use of sugammadex was associated with a clinically and statistically significant lower incidence of major pulmonary complications.
32282427	3	41	theme	Multicenter	487:497	arg1	Outcomes					513:520	Twelve U.S. Multicenter Perioperative Outcomes	475:520	METHODS Twelve U.S. Multicenter Perioperative Outcomes Group hospitals were included in a multicenter observational matched-cohort study of surgical cases between January 2014 and August 2018.	467:658	METHODS Twelve U.S. Multicenter Perioperative Outcomes Group hospitals were included in a multicenter observational matched-cohort study of surgical cases between January 2014 and August 2018.
32282427	2	42	theme	major	436:440	arg1	complications					452:464	major pulmonary complications	436:464	major pulmonary complications	436:464	The authors hypothesized that the choice of neuromuscular blockade reversal (neostigmine vs. sugammadex) may be associated with a lower incidence of major pulmonary complications.
32282427	7	43	theme	pulmonary	1279:1287	arg1	congestion					1337:1346	pulmonary congestion	1327:1346	pulmonary congestion	1327:1346	The composite primary outcome was major postoperative pulmonary complications, defined as pneumonia, respiratory failure, or other pulmonary complications (including pneumonitis; pulmonary congestion; iatrogenic pulmonary embolism, infarction, or pneumothorax).
32282427	7	43	theme	pulmonary	1279:1287	arg1	infarction					1380:1389	infarction	1380:1389	infarction	1380:1389	The composite primary outcome was major postoperative pulmonary complications, defined as pneumonia, respiratory failure, or other pulmonary complications (including pneumonitis; pulmonary congestion; iatrogenic pulmonary embolism, infarction, or pneumothorax).
32282427	7	43	theme	pulmonary	1279:1287	arg1	complications					1289:1301	other pulmonary complications	1273:1301	other pulmonary complications (including pneumonitis; pulmonary congestion; iatrogenic pulmonary embolism, infarction, or pneumothorax)	1273:1407	The composite primary outcome was major postoperative pulmonary complications, defined as pneumonia, respiratory failure, or other pulmonary complications (including pneumonitis; pulmonary congestion; iatrogenic pulmonary embolism, infarction, or pneumothorax).
32282427	7	43	theme	pulmonary	1279:1287	arg1	pneumothorax					1395:1406	pneumothorax	1395:1406	pneumothorax	1395:1406	The composite primary outcome was major postoperative pulmonary complications, defined as pneumonia, respiratory failure, or other pulmonary complications (including pneumonitis; pulmonary congestion; iatrogenic pulmonary embolism, infarction, or pneumothorax).
32282427	7	43	theme	pulmonary	1279:1287	arg1	pneumonitis					1314:1324	pneumonitis	1314:1324	pneumonitis	1314:1324	The composite primary outcome was major postoperative pulmonary complications, defined as pneumonia, respiratory failure, or other pulmonary complications (including pneumonitis; pulmonary congestion; iatrogenic pulmonary embolism, infarction, or pneumothorax).
32282427	7	43	theme	pulmonary	1279:1287	arg1	embolism					1370:1377	iatrogenic pulmonary embolism	1349:1377	iatrogenic pulmonary embolism	1349:1377	The composite primary outcome was major postoperative pulmonary complications, defined as pneumonia, respiratory failure, or other pulmonary complications (including pneumonitis; pulmonary congestion; iatrogenic pulmonary embolism, infarction, or pneumothorax).
32282427	3	44	dep	METHODS	467:473	arg1	included					543:550	included	543:550	were included in a multicenter observational matched-cohort study of surgical cases between January 2014 and August 2018	538:657	METHODS Twelve U.S. Multicenter Perioperative Outcomes Group hospitals were included in a multicenter observational matched-cohort study of surgical cases between January 2014 and August 2018.
32282427	3	44	dep	METHODS	467:473	arg1	Outcomes					513:520	Twelve U.S. Multicenter Perioperative Outcomes	475:520	METHODS Twelve U.S. Multicenter Perioperative Outcomes Group hospitals were included in a multicenter observational matched-cohort study of surgical cases between January 2014 and August 2018.	467:658	METHODS Twelve U.S. Multicenter Perioperative Outcomes Group hospitals were included in a multicenter observational matched-cohort study of surgical cases between January 2014 and August 2018.
32282427	12	45	dep	0.77	2016:2019	arg1	to					2013:2014	to	2013:2014	to	2013:2014	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	0	46	theme	Neuromuscular	46:58	arg1	Blockade					60:67	Neuromuscular Blockade	46:67	Neuromuscular Blockade	46:67	Sugammadex versus Neostigmine for Reversal of Neuromuscular Blockade and Postoperative Pulmonary Complications (STRONGER): A Multicenter Matched Cohort Analysis.
32282427	13	47	theme	generalizable	2244:2256	arg1	cohort					2258:2263	a generalizable cohort	2242:2263	a generalizable cohort of adult patients undergoing inpatient surgery at U.S. hospitals	2242:2328	CONCLUSIONS Among a generalizable cohort of adult patients undergoing inpatient surgery at U.S. hospitals, the use of sugammadex was associated with a clinically and statistically significant lower incidence of major pulmonary complications.
32282427	13	47	theme	generalizable	2244:2256	arg1	use					2335:2337	the use	2331:2337	the use of sugammadex	2331:2351	CONCLUSIONS Among a generalizable cohort of adult patients undergoing inpatient surgery at U.S. hospitals, the use of sugammadex was associated with a clinically and statistically significant lower incidence of major pulmonary complications.
32282427	11	48	dep	pneumonia	1774:1782	arg1	%					1788:1788	1.3%	1785:1788	1.3%	1785:1788	A total of 796 (1.7%) patients had pneumonia (1.3% vs. 2.2%), and 582 (1.3%) respiratory failure (0.8% vs. 1.7%).
32282427	11	48	dep	pneumonia	1774:1782	arg1	%					1797:1797	2.2%	1794:1797	2.2%	1794:1797	A total of 796 (1.7%) patients had pneumonia (1.3% vs. 2.2%), and 582 (1.3%) respiratory failure (0.8% vs. 1.7%).
32282427	12	49	theme	multivariable	1856:1868	arg1	analysis					1870:1877	multivariable analysis	1856:1877	multivariable analysis	1856:1877	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	3	50	theme	observational	569:581	arg1	study					598:602	a multicenter observational matched-cohort study	555:602	a multicenter observational matched-cohort study of surgical cases between January 2014 and August 2018	555:657	METHODS Twelve U.S. Multicenter Perioperative Outcomes Group hospitals were included in a multicenter observational matched-cohort study of surgical cases between January 2014 and August 2018.
32282427	1	51	theme	major	257:261	arg1	complication					273:284	a major pulmonary complication	255:284	a major pulmonary complication	255:284	BACKGROUND Five percent of adult patients undergoing noncardiac inpatient surgery experience a major pulmonary complication.
32282427	13	52	theme	major	2435:2439	arg1	complications					2451:2463	major pulmonary complications	2435:2463	major pulmonary complications	2435:2463	CONCLUSIONS Among a generalizable cohort of adult patients undergoing inpatient surgery at U.S. hospitals, the use of sugammadex was associated with a clinically and statistically significant lower incidence of major pulmonary complications.
32282427	0	53	dep	Sugammadex	0:9	arg1	Analysis					152:159	A Multicenter Matched Cohort Analysis	123:159	Sugammadex versus Neostigmine for Reversal of Neuromuscular Blockade and Postoperative Pulmonary Complications (STRONGER): A Multicenter Matched Cohort Analysis.	0:160	Sugammadex versus Neostigmine for Reversal of Neuromuscular Blockade and Postoperative Pulmonary Complications (STRONGER): A Multicenter Matched Cohort Analysis.
32282427	4	54	theme	elective	686:693	arg1	procedures					725:734	elective inpatient noncardiac surgical procedures	686:734	elective inpatient noncardiac surgical procedures	686:734	Adult patients undergoing elective inpatient noncardiac surgical procedures with general anesthesia and endotracheal intubation receiving a nondepolarizing neuromuscular blockade agent and reversal were included.
32282427	10	55	theme	composite	1673:1681	arg1	outcome					1691:1697	the composite primary outcome	1669:1697	the composite primary outcome (3.5% sugammadex vs. 4.8% neostigmine)	1669:1736	Out of 45,712 patients studied, 1,892 (4.1%) were diagnosed with the composite primary outcome (3.5% sugammadex vs. 4.8% neostigmine).
32282427	7	56	theme	respiratory	1249:1259	arg1	failure					1261:1267	respiratory failure	1249:1267	respiratory failure	1249:1267	The composite primary outcome was major postoperative pulmonary complications, defined as pneumonia, respiratory failure, or other pulmonary complications (including pneumonitis; pulmonary congestion; iatrogenic pulmonary embolism, infarction, or pneumothorax).
32282427	13	57	theme	significant	2404:2414	arg1	incidence					2422:2430	a clinically and statistically significant lower incidence	2373:2430	a clinically and statistically significant lower incidence of major pulmonary complications	2373:2463	CONCLUSIONS Among a generalizable cohort of adult patients undergoing inpatient surgery at U.S. hospitals, the use of sugammadex was associated with a clinically and statistically significant lower incidence of major pulmonary complications.
32282427	3	58	theme	surgical	607:614	arg1	cases					616:620	surgical cases	607:620	surgical cases between January 2014 and August 2018	607:657	METHODS Twelve U.S. Multicenter Perioperative Outcomes Group hospitals were included in a multicenter observational matched-cohort study of surgical cases between January 2014 and August 2018.
32282427	12	59	dep	0.56	2192:2195	arg1	to					2189:2190	to	2189:2190	to	2189:2190	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	8	60	theme	respiratory	1472:1482	arg1	failure					1484:1490	respiratory failure	1472:1490	respiratory failure	1472:1490	Secondary outcomes focused on the components of pneumonia and respiratory failure.
32282427	10	61	theme	3.5	1700:1702	arg1	%					1703:1703	%	1703:1703	%	1703:1703	Out of 45,712 patients studied, 1,892 (4.1%) were diagnosed with the composite primary outcome (3.5% sugammadex vs. 4.8% neostigmine).
32282427	13	62	theme	pulmonary	2441:2449	arg1	complications					2451:2463	major pulmonary complications	2435:2463	major pulmonary complications	2435:2463	CONCLUSIONS Among a generalizable cohort of adult patients undergoing inpatient surgery at U.S. hospitals, the use of sugammadex was associated with a clinically and statistically significant lower incidence of major pulmonary complications.
32282427	4	63	theme	general	741:747	arg1	anesthesia					749:758	general anesthesia	741:758	general anesthesia	741:758	Adult patients undergoing elective inpatient noncardiac surgical procedures with general anesthesia and endotracheal intubation receiving a nondepolarizing neuromuscular blockade agent and reversal were included.
32282427	7	64	theme	pulmonary	1360:1368	arg1	embolism					1370:1377	iatrogenic pulmonary embolism	1349:1377	iatrogenic pulmonary embolism	1349:1377	The composite primary outcome was major postoperative pulmonary complications, defined as pneumonia, respiratory failure, or other pulmonary complications (including pneumonitis; pulmonary congestion; iatrogenic pulmonary embolism, infarction, or pneumothorax).
32282427	2	65	theme	reversal	354:361	arg1	choice					321:326	the choice	317:326	the choice of neuromuscular blockade reversal (neostigmine vs. sugammadex)	317:390	The authors hypothesized that the choice of neuromuscular blockade reversal (neostigmine vs. sugammadex) may be associated with a lower incidence of major pulmonary complications.
32282427	1	66	theme	adult	189:193	arg1	patients					195:202	adult patients	189:202	adult patients undergoing noncardiac inpatient surgery	189:242	BACKGROUND Five percent of adult patients undergoing noncardiac inpatient surgery experience a major pulmonary complication.
32282427	12	67	theme	odds	1982:1985	arg1	ratio					1987:1991	adjusted odds ratio	1973:1991	adjusted odds ratio	1973:1991	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	10	68	theme	4.8	1720:1722	arg1	%					1723:1723	%	1723:1723	%	1723:1723	Out of 45,712 patients studied, 1,892 (4.1%) were diagnosed with the composite primary outcome (3.5% sugammadex vs. 4.8% neostigmine).
32282427	7	69	theme	postoperative	1188:1200	arg1	complications					1212:1224	major postoperative pulmonary complications	1182:1224	major postoperative pulmonary complications	1182:1224	The composite primary outcome was major postoperative pulmonary complications, defined as pneumonia, respiratory failure, or other pulmonary complications (including pneumonitis; pulmonary congestion; iatrogenic pulmonary embolism, infarction, or pneumothorax).
32282427	7	69	theme	postoperative	1188:1200	arg1	outcome					1170:1176	The composite primary outcome	1148:1176	The composite primary outcome	1148:1176	The composite primary outcome was major postoperative pulmonary complications, defined as pneumonia, respiratory failure, or other pulmonary complications (including pneumonitis; pulmonary congestion; iatrogenic pulmonary embolism, infarction, or pneumothorax).
32282427	2	70	theme	neuromuscular	331:343	arg1	reversal					354:361	neuromuscular blockade reversal	331:361	neuromuscular blockade reversal (neostigmine vs. sugammadex)	331:390	The authors hypothesized that the choice of neuromuscular blockade reversal (neostigmine vs. sugammadex) may be associated with a lower incidence of major pulmonary complications.
32282427	4	71	theme	noncardiac	705:714	arg1	procedures					725:734	elective inpatient noncardiac surgical procedures	686:734	elective inpatient noncardiac surgical procedures	686:734	Adult patients undergoing elective inpatient noncardiac surgical procedures with general anesthesia and endotracheal intubation receiving a nondepolarizing neuromuscular blockade agent and reversal were included.
32282427	0	72	theme	Cohort	145:150	arg1	Analysis					152:159	A Multicenter Matched Cohort Analysis	123:159	Sugammadex versus Neostigmine for Reversal of Neuromuscular Blockade and Postoperative Pulmonary Complications (STRONGER): A Multicenter Matched Cohort Analysis.	0:160	Sugammadex versus Neostigmine for Reversal of Neuromuscular Blockade and Postoperative Pulmonary Complications (STRONGER): A Multicenter Matched Cohort Analysis.
32282427	5	73	theme	neuromuscular	982:994	arg1	blockade					996:1003	neuromuscular blockade	982:1003	neuromuscular blockade agent (rocuronium vs. vecuronium)	982:1037	Exact matching criteria included institution, sex, age, comorbidities, obesity, surgical procedure type, and neuromuscular blockade agent (rocuronium vs. vecuronium).
32282427	5	74	theme	surgical	953:960	arg1	procedure					962:970	surgical procedure	953:970	surgical procedure	953:970	Exact matching criteria included institution, sex, age, comorbidities, obesity, surgical procedure type, and neuromuscular blockade agent (rocuronium vs. vecuronium).
32282427	5	74	theme	surgical	953:960	arg1	age					924:926	age	924:926	age	924:926	Exact matching criteria included institution, sex, age, comorbidities, obesity, surgical procedure type, and neuromuscular blockade agent (rocuronium vs. vecuronium).
32282427	12	75	theme	pulmonary	1948:1956	arg1	complications					1958:1970	pulmonary complications	1948:1970	pulmonary complications	1948:1970	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	6	76	theme	intraoperative	1063:1076	arg1	factors					1078:1084	Other preoperative and intraoperative factors	1040:1084	Other preoperative and intraoperative factors	1040:1084	Other preoperative and intraoperative factors were compared and adjusted in the case of residual imbalance.
32282427	12	77	theme	odds	2063:2066	arg1	ratio					2068:2072	adjusted odds ratio	2054:2072	adjusted odds ratio	2054:2072	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	12	77	theme	odds	2063:2066	arg1	pneumonia					2043:2051	pneumonia	2043:2051	pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62)	2043:2101	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	12	78	dep	reduced	1932:1938	arg1	ratio					1987:1991	adjusted odds ratio	1973:1991	adjusted odds ratio	1973:1991	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	7	79	theme	primary	1162:1168	arg1	complications					1212:1224	major postoperative pulmonary complications	1182:1224	major postoperative pulmonary complications	1182:1224	The composite primary outcome was major postoperative pulmonary complications, defined as pneumonia, respiratory failure, or other pulmonary complications (including pneumonitis; pulmonary congestion; iatrogenic pulmonary embolism, infarction, or pneumothorax).
32282427	7	79	theme	primary	1162:1168	arg1	outcome					1170:1176	The composite primary outcome	1148:1176	The composite primary outcome	1148:1176	The composite primary outcome was major postoperative pulmonary complications, defined as pneumonia, respiratory failure, or other pulmonary complications (including pneumonitis; pulmonary congestion; iatrogenic pulmonary embolism, infarction, or pneumothorax).
32282427	6	80	theme	preoperative	1046:1057	arg1	factors					1078:1084	Other preoperative and intraoperative factors	1040:1084	Other preoperative and intraoperative factors	1040:1084	Other preoperative and intraoperative factors were compared and adjusted in the case of residual imbalance.
32282427	4	81	theme	neuromuscular	816:828	arg1	blockade					830:837	a nondepolarizing neuromuscular blockade agent and reversal	798:856	a nondepolarizing neuromuscular blockade agent and reversal	798:856	Adult patients undergoing elective inpatient noncardiac surgical procedures with general anesthesia and endotracheal intubation receiving a nondepolarizing neuromuscular blockade agent and reversal were included.
32282427	10	82	dep	outcome	1691:1697	arg1	neostigmine					1725:1735	4.8% neostigmine	1720:1735	4.8% neostigmine	1720:1735	Out of 45,712 patients studied, 1,892 (4.1%) were diagnosed with the composite primary outcome (3.5% sugammadex vs. 4.8% neostigmine).
32282427	10	82	dep	outcome	1691:1697	arg1	sugammadex					1705:1714	3.5% sugammadex	1700:1714	3.5% sugammadex	1700:1714	Out of 45,712 patients studied, 1,892 (4.1%) were diagnosed with the composite primary outcome (3.5% sugammadex vs. 4.8% neostigmine).
32282427	5	83	theme	Exact	873:877	arg1	criteria					888:895	Exact matching criteria	873:895	Exact matching criteria	873:895	Exact matching criteria included institution, sex, age, comorbidities, obesity, surgical procedure type, and neuromuscular blockade agent (rocuronium vs. vecuronium).
32282427	12	84	theme	adjusted	2054:2061	arg1	ratio					2068:2072	adjusted odds ratio	2054:2072	adjusted odds ratio	2054:2072	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	12	84	theme	adjusted	2054:2061	arg1	pneumonia					2043:2051	pneumonia	2043:2051	pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62)	2043:2101	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	5	85	theme	blockade	996:1003	arg1	agent					1005:1009	neuromuscular blockade agent	982:1009	neuromuscular blockade agent (rocuronium vs. vecuronium)	982:1037	Exact matching criteria included institution, sex, age, comorbidities, obesity, surgical procedure type, and neuromuscular blockade agent (rocuronium vs. vecuronium).
32282427	6	86	theme	residual	1128:1135	arg1	imbalance					1137:1145	residual imbalance	1128:1145	residual imbalance	1128:1145	Other preoperative and intraoperative factors were compared and adjusted in the case of residual imbalance.
32282427	7	87	theme	iatrogenic	1349:1358	arg1	embolism					1370:1377	iatrogenic pulmonary embolism	1349:1377	iatrogenic pulmonary embolism	1349:1377	The composite primary outcome was major postoperative pulmonary complications, defined as pneumonia, respiratory failure, or other pulmonary complications (including pneumonitis; pulmonary congestion; iatrogenic pulmonary embolism, infarction, or pneumothorax).
32282427	1	88	theme	BACKGROUND	162:171	arg1	percent					178:184	BACKGROUND Five percent	162:184	BACKGROUND Five percent of adult patients undergoing noncardiac inpatient surgery	162:242	BACKGROUND Five percent of adult patients undergoing noncardiac inpatient surgery experience a major pulmonary complication.
32282427	12	89	theme	pneumonia	2043:2051	arg1	risk					2035:2038	risk	2035:2038	risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62)	2035:2101	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	11	90	theme	respiratory	1816:1826	arg1	failure					1828:1834	582 (1.3%) respiratory failure	1805:1834	582 (1.3%) respiratory failure (0.8% vs. 1.7%)	1805:1850	A total of 796 (1.7%) patients had pneumonia (1.3% vs. 2.2%), and 582 (1.3%) respiratory failure (0.8% vs. 1.7%).
32282427	12	91	theme	adjusted	2149:2156	arg1	0.56					2192:2195	0.56	2192:2195	0.56	2192:2195	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	12	91	theme	adjusted	2149:2156	arg1	%					2178:2178	0.45; 95% CI	2170:2181	0.45; 95% CI	2170:2181	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	12	91	theme	adjusted	2149:2156	arg1	ratio					2163:2167	adjusted odds ratio	2149:2167	adjusted odds ratio	2149:2167	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	8	92	theme	failure	1484:1490	arg1	components					1444:1453	the components	1440:1453	the components of pneumonia and respiratory failure	1440:1490	Secondary outcomes focused on the components of pneumonia and respiratory failure.
32282427	12	93	theme	respiratory	2128:2138	arg1	failure					2140:2146	respiratory failure	2128:2146	respiratory failure	2128:2146	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	2	94	theme	lower	417:421	arg1	incidence					423:431	a lower incidence	415:431	a lower incidence of major pulmonary complications	415:464	The authors hypothesized that the choice of neuromuscular blockade reversal (neostigmine vs. sugammadex) may be associated with a lower incidence of major pulmonary complications.
32282427	13	95	theme	complications	2451:2463	arg1	incidence					2422:2430	a clinically and statistically significant lower incidence	2373:2430	a clinically and statistically significant lower incidence of major pulmonary complications	2373:2463	CONCLUSIONS Among a generalizable cohort of adult patients undergoing inpatient surgery at U.S. hospitals, the use of sugammadex was associated with a clinically and statistically significant lower incidence of major pulmonary complications.
32282427	0	96	dep	Complications	97:109	arg1	STRONGER					112:119	STRONGER	112:119	STRONGER	112:119	Sugammadex versus Neostigmine for Reversal of Neuromuscular Blockade and Postoperative Pulmonary Complications (STRONGER): A Multicenter Matched Cohort Analysis.
32282427	13	97	theme	sugammadex	2342:2351	arg1	cohort					2258:2263	a generalizable cohort	2242:2263	a generalizable cohort of adult patients undergoing inpatient surgery at U.S. hospitals	2242:2328	CONCLUSIONS Among a generalizable cohort of adult patients undergoing inpatient surgery at U.S. hospitals, the use of sugammadex was associated with a clinically and statistically significant lower incidence of major pulmonary complications.
32282427	13	97	theme	sugammadex	2342:2351	arg1	use					2335:2337	the use	2331:2337	the use of sugammadex	2331:2351	CONCLUSIONS Among a generalizable cohort of adult patients undergoing inpatient surgery at U.S. hospitals, the use of sugammadex was associated with a clinically and statistically significant lower incidence of major pulmonary complications.
32282427	12	98	theme	sugammadex	1880:1889	arg1	administration					1891:1904	sugammadex administration	1880:1904	sugammadex administration	1880:1904	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	4	99	dep	blockade	830:837	arg1	reversal					849:856	reversal	849:856	reversal	849:856	Adult patients undergoing elective inpatient noncardiac surgical procedures with general anesthesia and endotracheal intubation receiving a nondepolarizing neuromuscular blockade agent and reversal were included.
32282427	4	99	dep	blockade	830:837	arg1	agent					839:843	agent	839:843	agent	839:843	Adult patients undergoing elective inpatient noncardiac surgical procedures with general anesthesia and endotracheal intubation receiving a nondepolarizing neuromuscular blockade agent and reversal were included.
32282427	5	100	dep	agent	1005:1009	arg1	vecuronium					1027:1036	vecuronium	1027:1036	vecuronium	1027:1036	Exact matching criteria included institution, sex, age, comorbidities, obesity, surgical procedure type, and neuromuscular blockade agent (rocuronium vs. vecuronium).
32282427	5	100	dep	agent	1005:1009	arg1	rocuronium					1012:1021	rocuronium	1012:1021	rocuronium	1012:1021	Exact matching criteria included institution, sex, age, comorbidities, obesity, surgical procedure type, and neuromuscular blockade agent (rocuronium vs. vecuronium).
32282427	2	101	theme	pulmonary	442:450	arg1	complications					452:464	major pulmonary complications	436:464	major pulmonary complications	436:464	The authors hypothesized that the choice of neuromuscular blockade reversal (neostigmine vs. sugammadex) may be associated with a lower incidence of major pulmonary complications.
32282427	7	102	theme	other	1273:1277	arg1	congestion					1337:1346	pulmonary congestion	1327:1346	pulmonary congestion	1327:1346	The composite primary outcome was major postoperative pulmonary complications, defined as pneumonia, respiratory failure, or other pulmonary complications (including pneumonitis; pulmonary congestion; iatrogenic pulmonary embolism, infarction, or pneumothorax).
32282427	7	102	theme	other	1273:1277	arg1	infarction					1380:1389	infarction	1380:1389	infarction	1380:1389	The composite primary outcome was major postoperative pulmonary complications, defined as pneumonia, respiratory failure, or other pulmonary complications (including pneumonitis; pulmonary congestion; iatrogenic pulmonary embolism, infarction, or pneumothorax).
32282427	7	102	theme	other	1273:1277	arg1	complications					1289:1301	other pulmonary complications	1273:1301	other pulmonary complications (including pneumonitis; pulmonary congestion; iatrogenic pulmonary embolism, infarction, or pneumothorax)	1273:1407	The composite primary outcome was major postoperative pulmonary complications, defined as pneumonia, respiratory failure, or other pulmonary complications (including pneumonitis; pulmonary congestion; iatrogenic pulmonary embolism, infarction, or pneumothorax).
32282427	7	102	theme	other	1273:1277	arg1	pneumothorax					1395:1406	pneumothorax	1395:1406	pneumothorax	1395:1406	The composite primary outcome was major postoperative pulmonary complications, defined as pneumonia, respiratory failure, or other pulmonary complications (including pneumonitis; pulmonary congestion; iatrogenic pulmonary embolism, infarction, or pneumothorax).
32282427	7	102	theme	other	1273:1277	arg1	pneumonitis					1314:1324	pneumonitis	1314:1324	pneumonitis	1314:1324	The composite primary outcome was major postoperative pulmonary complications, defined as pneumonia, respiratory failure, or other pulmonary complications (including pneumonitis; pulmonary congestion; iatrogenic pulmonary embolism, infarction, or pneumothorax).
32282427	7	102	theme	other	1273:1277	arg1	embolism					1370:1377	iatrogenic pulmonary embolism	1349:1377	iatrogenic pulmonary embolism	1349:1377	The composite primary outcome was major postoperative pulmonary complications, defined as pneumonia, respiratory failure, or other pulmonary complications (including pneumonitis; pulmonary congestion; iatrogenic pulmonary embolism, infarction, or pneumothorax).
32282427	1	103	theme	inpatient	226:234	arg1	surgery					236:242	noncardiac inpatient surgery	215:242	noncardiac inpatient surgery	215:242	BACKGROUND Five percent of adult patients undergoing noncardiac inpatient surgery experience a major pulmonary complication.
32282427	12	104	dep	ratio	2068:2072	arg1	0.62					2097:2100	0.62	2097:2100	0.62	2097:2100	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	12	104	dep	ratio	2068:2072	arg1	%					2083:2083	95% CI	2081:2086	95% CI	2081:2086	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	12	104	dep	ratio	2068:2072	arg1	0.53					2075:2078	0.53	2075:2078	0.53	2075:2078	In multivariable analysis, sugammadex administration was associated with a 30% reduced risk of pulmonary complications (adjusted odds ratio, 0.70; 95% CI, 0.63 to 0.77), 47% reduced risk of pneumonia (adjusted odds ratio, 0.53; 95% CI, 0.44 to 0.62), and 55% reduced risk of respiratory failure (adjusted odds ratio, 0.45; 95% CI, 0.37 to 0.56), compared to neostigmine.
32282427	3	105	theme	Perioperative	499:511	arg1	Outcomes					513:520	Twelve U.S. Multicenter Perioperative Outcomes	475:520	METHODS Twelve U.S. Multicenter Perioperative Outcomes Group hospitals were included in a multicenter observational matched-cohort study of surgical cases between January 2014 and August 2018.	467:658	METHODS Twelve U.S. Multicenter Perioperative Outcomes Group hospitals were included in a multicenter observational matched-cohort study of surgical cases between January 2014 and August 2018.
32282427	0	106	theme	Blockade	60:67	arg1	Complications					97:109	Postoperative Pulmonary Complications	73:109	Postoperative Pulmonary Complications (STRONGER)	73:120	Sugammadex versus Neostigmine for Reversal of Neuromuscular Blockade and Postoperative Pulmonary Complications (STRONGER): A Multicenter Matched Cohort Analysis.
32282427	0	106	theme	Blockade	60:67	arg1	Reversal					34:41	Reversal	34:41	Reversal of Neuromuscular Blockade	34:67	Sugammadex versus Neostigmine for Reversal of Neuromuscular Blockade and Postoperative Pulmonary Complications (STRONGER): A Multicenter Matched Cohort Analysis.
32282427	9	107	theme	patients	1511:1518	arg1	RESULTS					1493:1499	RESULTS	1493:1499	RESULTS Of 30,026 patients receiving sugammadex, 22,856	1493:1547	RESULTS Of 30,026 patients receiving sugammadex, 22,856 were matched to 22,856 patients receiving neostigmine.
32282427	0	108	theme	Matched	137:143	arg1	Analysis					152:159	A Multicenter Matched Cohort Analysis	123:159	Sugammadex versus Neostigmine for Reversal of Neuromuscular Blockade and Postoperative Pulmonary Complications (STRONGER): A Multicenter Matched Cohort Analysis.	0:160	Sugammadex versus Neostigmine for Reversal of Neuromuscular Blockade and Postoperative Pulmonary Complications (STRONGER): A Multicenter Matched Cohort Analysis.
32282427	8	109	theme	Secondary	1410:1418	arg1	outcomes					1420:1427	Secondary outcomes	1410:1427	Secondary outcomes	1410:1427	Secondary outcomes focused on the components of pneumonia and respiratory failure.
32282427	3	110	theme	U.S.	482:485	arg1	Outcomes					513:520	Twelve U.S. Multicenter Perioperative Outcomes	475:520	METHODS Twelve U.S. Multicenter Perioperative Outcomes Group hospitals were included in a multicenter observational matched-cohort study of surgical cases between January 2014 and August 2018.	467:658	METHODS Twelve U.S. Multicenter Perioperative Outcomes Group hospitals were included in a multicenter observational matched-cohort study of surgical cases between January 2014 and August 2018.
32282427	4	111	theme	inpatient	695:703	arg1	procedures					725:734	elective inpatient noncardiac surgical procedures	686:734	elective inpatient noncardiac surgical procedures	686:734	Adult patients undergoing elective inpatient noncardiac surgical procedures with general anesthesia and endotracheal intubation receiving a nondepolarizing neuromuscular blockade agent and reversal were included.
32282427	10	112	theme	primary	1683:1689	arg1	outcome					1691:1697	the composite primary outcome	1669:1697	the composite primary outcome (3.5% sugammadex vs. 4.8% neostigmine)	1669:1736	Out of 45,712 patients studied, 1,892 (4.1%) were diagnosed with the composite primary outcome (3.5% sugammadex vs. 4.8% neostigmine).
32282427	3	113	theme	matched-cohort	583:596	arg1	study					598:602	a multicenter observational matched-cohort study	555:602	a multicenter observational matched-cohort study of surgical cases between January 2014 and August 2018	555:657	METHODS Twelve U.S. Multicenter Perioperative Outcomes Group hospitals were included in a multicenter observational matched-cohort study of surgical cases between January 2014 and August 2018.
32282427	1	114	theme	pulmonary	263:271	arg1	complication					273:284	a major pulmonary complication	255:284	a major pulmonary complication	255:284	BACKGROUND Five percent of adult patients undergoing noncardiac inpatient surgery experience a major pulmonary complication.
33041026	0	0	theme	high	63:66	arg1	treatment					73:81	high heat treatment	63:81	high heat treatment of Bactrian camel milk	63:104	The gut microbiota and its metabolites in mice are affected by high heat treatment of Bactrian camel milk.
33041026	0	1	from	microbiota	8:17	arg1	mice					42:45	mice	42:45	mice	42:45	The gut microbiota and its metabolites in mice are affected by high heat treatment of Bactrian camel milk.
33041026	4	2	theme	amino	632:636	arg1	acids					638:642	the amino acids	628:642	the amino acids content	628:650	Ultra-high-temperature treatment of samples significantly reduced levels of camel milk proteins, vitamin C, and lactose, but did not significantly alter the amino acids content.
33041026	6	3	contain	had	1078:1080	arg2	effects					1092:1098	different effects	1082:1098	different effects	1082:1098	High temperature/short time treatment had similar effects to UHT treatment on microbial diversity of camel milk; however, the low temperature/long time treatment had different effects.
33041026	6	3	contain	had	1078:1080	arg1	treatment					1068:1076	the low temperature/long time treatment	1038:1076	the low temperature/long time treatment	1038:1076	High temperature/short time treatment had similar effects to UHT treatment on microbial diversity of camel milk; however, the low temperature/long time treatment had different effects.
33041026	5	4	theme	acids	855:859	arg1	production					823:832	the production	819:832	the production of short-chain fatty acids as determined by gas chromatography-mass spectrometry	819:913	Analysis of 16S rRNA amplicon sequences demonstrated that the composition of the intestinal microbiota of mice fed different heat-treated camel milks changed, as did the production of short-chain fatty acids as determined by gas chromatography-mass spectrometry.
33041026	6	5	theme	High	916:919	arg1	treatment					944:952	High temperature/short time treatment	916:952	High temperature/short time treatment	916:952	High temperature/short time treatment had similar effects to UHT treatment on microbial diversity of camel milk; however, the low temperature/long time treatment had different effects.
33041026	9	6	theme	industrial	1416:1425	arg1	application					1427:1437	the potential industrial application	1402:1437	the potential industrial application of camel milk processing technologies	1402:1475	Our results could provide the basis for the potential industrial application of camel milk processing technologies.
33041026	5	7	theme	short-chain	837:847	arg1	acids					855:859	short-chain fatty acids	837:859	short-chain fatty acids	837:859	Analysis of 16S rRNA amplicon sequences demonstrated that the composition of the intestinal microbiota of mice fed different heat-treated camel milks changed, as did the production of short-chain fatty acids as determined by gas chromatography-mass spectrometry.
33041026	9	8	theme	processing	1453:1462	arg1	technologies					1464:1475	camel milk processing technologies	1442:1475	camel milk processing technologies	1442:1475	Our results could provide the basis for the potential industrial application of camel milk processing technologies.
33041026	6	9	theme	similar	958:964	arg1	effects					966:972	similar effects	958:972	similar effects to UHT treatment on microbial diversity of camel milk	958:1026	High temperature/short time treatment had similar effects to UHT treatment on microbial diversity of camel milk; however, the low temperature/long time treatment had different effects.
33041026	6	10	theme	different	1082:1090	arg1	effects					1092:1098	different effects	1082:1098	different effects	1082:1098	High temperature/short time treatment had similar effects to UHT treatment on microbial diversity of camel milk; however, the low temperature/long time treatment had different effects.
33041026	6	11	theme	UHT	977:979	arg1	treatment					981:989	UHT treatment	977:989	UHT treatment	977:989	High temperature/short time treatment had similar effects to UHT treatment on microbial diversity of camel milk; however, the low temperature/long time treatment had different effects.
33041026	4	12	theme	camel	551:555	arg1	proteins					562:569	camel milk proteins	551:569	camel milk proteins	551:569	Ultra-high-temperature treatment of samples significantly reduced levels of camel milk proteins, vitamin C, and lactose, but did not significantly alter the amino acids content.
33041026	0	13	theme	heat	68:71	arg1	treatment					73:81	high heat treatment	63:81	high heat treatment of Bactrian camel milk	63:104	The gut microbiota and its metabolites in mice are affected by high heat treatment of Bactrian camel milk.
33041026	6	14	theme	low	1042:1044	arg1	treatment					1068:1076	the low temperature/long time treatment	1038:1076	the low temperature/long time treatment	1038:1076	High temperature/short time treatment had similar effects to UHT treatment on microbial diversity of camel milk; however, the low temperature/long time treatment had different effects.
33041026	8	15	theme	different	1234:1242	arg1	treatments					1249:1258	different heat treatments	1234:1258	different heat treatments	1234:1258	These results demonstrated that different heat treatments not only resulted in some nutrient loss, but also changed the proliferation of some probiotic genera.
33041026	0	16	theme	Bactrian	86:93	arg1	milk					101:104	Bactrian camel milk	86:104	Bactrian camel milk	86:104	The gut microbiota and its metabolites in mice are affected by high heat treatment of Bactrian camel milk.
33041026	5	17	theme	fatty	849:853	arg1	acids					855:859	short-chain fatty acids	837:859	short-chain fatty acids	837:859	Analysis of 16S rRNA amplicon sequences demonstrated that the composition of the intestinal microbiota of mice fed different heat-treated camel milks changed, as did the production of short-chain fatty acids as determined by gas chromatography-mass spectrometry.
33041026	5	18	theme	heat-treated	778:789	arg1	milks					797:801	different heat-treated camel milks	768:801	different heat-treated camel milks	768:801	Analysis of 16S rRNA amplicon sequences demonstrated that the composition of the intestinal microbiota of mice fed different heat-treated camel milks changed, as did the production of short-chain fatty acids as determined by gas chromatography-mass spectrometry.
33041026	4	19	theme	C	580:580	arg1	levels					541:546	levels	541:546	levels of camel milk proteins, vitamin C, and lactose	541:593	Ultra-high-temperature treatment of samples significantly reduced levels of camel milk proteins, vitamin C, and lactose, but did not significantly alter the amino acids content.
33041026	3	20	theme	heat	459:462	arg1	treatment					464:472	heat treatment	459:472	heat treatment	459:472	The results showed that the nutrient composition of camel milk could be influenced by heat treatment.
33041026	9	21	theme	potential	1406:1414	arg1	application					1427:1437	the potential industrial application	1402:1437	the potential industrial application of camel milk processing technologies	1402:1475	Our results could provide the basis for the potential industrial application of camel milk processing technologies.
33041026	1	22	theme	common	134:139	arg1	method					141:146	the most common method	125:146	the most common method used to make milk safe	125:169	Heat treatment is the most common method used to make milk safe; however, it leads to changes in the organoleptic and nutritional properties of milk.
33041026	1	22	theme	common	134:139	arg1	treatment					112:120	Heat treatment	107:120	Heat treatment	107:120	Heat treatment is the most common method used to make milk safe; however, it leads to changes in the organoleptic and nutritional properties of milk.
33041026	5	23	theme	amplicon	674:681	arg1	sequences					683:691	16S rRNA amplicon sequences	665:691	16S rRNA amplicon sequences	665:691	Analysis of 16S rRNA amplicon sequences demonstrated that the composition of the intestinal microbiota of mice fed different heat-treated camel milks changed, as did the production of short-chain fatty acids as determined by gas chromatography-mass spectrometry.
33041026	0	24	from	metabolites	27:37	arg1	mice					42:45	mice	42:45	mice	42:45	The gut microbiota and its metabolites in mice are affected by high heat treatment of Bactrian camel milk.
33041026	2	25	from	effects	293:299	arg1	microbiota					347:356	microbiota	347:356	microbiota	347:356	This study aimed to investigate the effects of different heat treatments on nutrients and microbiota of camel milk.
33041026	2	25	from	effects	293:299	arg1	nutrients					333:341	nutrients	333:341	nutrients	333:341	This study aimed to investigate the effects of different heat treatments on nutrients and microbiota of camel milk.
33041026	9	26	theme	technologies	1464:1475	arg1	application					1427:1437	the potential industrial application	1402:1437	the potential industrial application of camel milk processing technologies	1402:1475	Our results could provide the basis for the potential industrial application of camel milk processing technologies.
33041026	5	27	theme	rRNA	669:672	arg1	sequences					683:691	16S rRNA amplicon sequences	665:691	16S rRNA amplicon sequences	665:691	Analysis of 16S rRNA amplicon sequences demonstrated that the composition of the intestinal microbiota of mice fed different heat-treated camel milks changed, as did the production of short-chain fatty acids as determined by gas chromatography-mass spectrometry.
33041026	0	28	theme	gut	4:6	arg1	microbiota					8:17	The gut microbiota	0:17	The gut microbiota	0:17	The gut microbiota and its metabolites in mice are affected by high heat treatment of Bactrian camel milk.
33041026	4	29	theme	samples	511:517	arg1	treatment					498:506	Ultra-high-temperature treatment	475:506	Ultra-high-temperature treatment of samples	475:517	Ultra-high-temperature treatment of samples significantly reduced levels of camel milk proteins, vitamin C, and lactose, but did not significantly alter the amino acids content.
33041026	6	30	from	effects	966:972	arg1	diversity					1004:1012	microbial diversity	994:1012	microbial diversity of camel milk	994:1026	High temperature/short time treatment had similar effects to UHT treatment on microbial diversity of camel milk; however, the low temperature/long time treatment had different effects.
33041026	6	31	theme	time	939:942	arg1	treatment					944:952	High temperature/short time treatment	916:952	High temperature/short time treatment	916:952	High temperature/short time treatment had similar effects to UHT treatment on microbial diversity of camel milk; however, the low temperature/long time treatment had different effects.
33041026	2	32	theme	treatments	319:328	arg1	effects					293:299	the effects	289:299	the effects of different heat treatments on nutrients and microbiota of camel milk	289:370	This study aimed to investigate the effects of different heat treatments on nutrients and microbiota of camel milk.
33041026	1	33	theme	organoleptic	208:219	arg1	properties					237:246	the organoleptic and nutritional properties	204:246	the organoleptic and nutritional properties of milk	204:254	Heat treatment is the most common method used to make milk safe; however, it leads to changes in the organoleptic and nutritional properties of milk.
33041026	7	34	from	level	1195:1199	arg1	abundance					1156:1164	the abundance	1152:1164	the abundance of key bacteria at the genus level	1152:1199	In addition, higher-temperature treatments changed the abundance of key bacteria at the genus level.
33041026	5	35	theme	gas	878:880	arg1	spectrometry					902:913	gas chromatography-mass spectrometry	878:913	gas chromatography-mass spectrometry	878:913	Analysis of 16S rRNA amplicon sequences demonstrated that the composition of the intestinal microbiota of mice fed different heat-treated camel milks changed, as did the production of short-chain fatty acids as determined by gas chromatography-mass spectrometry.
33041026	2	36	theme	heat	314:317	arg1	treatments					319:328	different heat treatments	304:328	different heat treatments	304:328	This study aimed to investigate the effects of different heat treatments on nutrients and microbiota of camel milk.
33041026	5	37	theme	mice	759:762	arg1	microbiota					745:754	the intestinal microbiota	730:754	the intestinal microbiota of mice fed different heat-treated camel milks	730:801	Analysis of 16S rRNA amplicon sequences demonstrated that the composition of the intestinal microbiota of mice fed different heat-treated camel milks changed, as did the production of short-chain fatty acids as determined by gas chromatography-mass spectrometry.
33041026	6	38	theme	temperature/short	921:937	arg1	treatment					944:952	High temperature/short time treatment	916:952	High temperature/short time treatment	916:952	High temperature/short time treatment had similar effects to UHT treatment on microbial diversity of camel milk; however, the low temperature/long time treatment had different effects.
33041026	0	39	theme	milk	101:104	arg1	treatment					73:81	high heat treatment	63:81	high heat treatment of Bactrian camel milk	63:104	The gut microbiota and its metabolites in mice are affected by high heat treatment of Bactrian camel milk.
33041026	5	40	theme	chromatography-mass	882:900	arg1	spectrometry					902:913	gas chromatography-mass spectrometry	878:913	gas chromatography-mass spectrometry	878:913	Analysis of 16S rRNA amplicon sequences demonstrated that the composition of the intestinal microbiota of mice fed different heat-treated camel milks changed, as did the production of short-chain fatty acids as determined by gas chromatography-mass spectrometry.
33041026	2	41	theme	camel	361:365	arg1	milk					367:370	camel milk	361:370	camel milk	361:370	This study aimed to investigate the effects of different heat treatments on nutrients and microbiota of camel milk.
33041026	2	42	theme	milk	367:370	arg1	microbiota					347:356	microbiota	347:356	microbiota	347:356	This study aimed to investigate the effects of different heat treatments on nutrients and microbiota of camel milk.
33041026	2	42	theme	milk	367:370	arg1	nutrients					333:341	nutrients	333:341	nutrients	333:341	This study aimed to investigate the effects of different heat treatments on nutrients and microbiota of camel milk.
33041026	1	43	theme	nutritional	225:235	arg1	properties					237:246	the organoleptic and nutritional properties	204:246	the organoleptic and nutritional properties of milk	204:254	Heat treatment is the most common method used to make milk safe; however, it leads to changes in the organoleptic and nutritional properties of milk.
33041026	0	44	theme	camel	95:99	arg1	milk					101:104	Bactrian camel milk	86:104	Bactrian camel milk	86:104	The gut microbiota and its metabolites in mice are affected by high heat treatment of Bactrian camel milk.
33041026	5	45	dep	production	823:832	arg1	determined					864:873	determined	864:873	determined by gas chromatography-mass spectrometry	864:913	Analysis of 16S rRNA amplicon sequences demonstrated that the composition of the intestinal microbiota of mice fed different heat-treated camel milks changed, as did the production of short-chain fatty acids as determined by gas chromatography-mass spectrometry.
33041026	3	46	theme	nutrient	401:408	arg1	composition					410:420	the nutrient composition	397:420	the nutrient composition of camel milk	397:434	The results showed that the nutrient composition of camel milk could be influenced by heat treatment.
33041026	7	47	theme	key	1169:1171	arg1	bacteria					1173:1180	key bacteria	1169:1180	key bacteria	1169:1180	In addition, higher-temperature treatments changed the abundance of key bacteria at the genus level.
33041026	4	48	theme	acids	638:642	arg1	content					644:650	the amino acids content	628:650	the amino acids content	628:650	Ultra-high-temperature treatment of samples significantly reduced levels of camel milk proteins, vitamin C, and lactose, but did not significantly alter the amino acids content.
33041026	5	49	theme	camel	791:795	arg1	milks					797:801	different heat-treated camel milks	768:801	different heat-treated camel milks	768:801	Analysis of 16S rRNA amplicon sequences demonstrated that the composition of the intestinal microbiota of mice fed different heat-treated camel milks changed, as did the production of short-chain fatty acids as determined by gas chromatography-mass spectrometry.
33041026	8	50	theme	nutrient	1286:1293	arg1	loss					1295:1298	some nutrient loss	1281:1298	some nutrient loss	1281:1298	These results demonstrated that different heat treatments not only resulted in some nutrient loss, but also changed the proliferation of some probiotic genera.
33041026	6	51	theme	milk	1023:1026	arg1	diversity					1004:1012	microbial diversity	994:1012	microbial diversity of camel milk	994:1026	High temperature/short time treatment had similar effects to UHT treatment on microbial diversity of camel milk; however, the low temperature/long time treatment had different effects.
33041026	4	52	theme	lactose	587:593	arg1	levels					541:546	levels	541:546	levels of camel milk proteins, vitamin C, and lactose	541:593	Ultra-high-temperature treatment of samples significantly reduced levels of camel milk proteins, vitamin C, and lactose, but did not significantly alter the amino acids content.
33041026	1	53	from	changes	193:199	arg1	properties					237:246	the organoleptic and nutritional properties	204:246	the organoleptic and nutritional properties of milk	204:254	Heat treatment is the most common method used to make milk safe; however, it leads to changes in the organoleptic and nutritional properties of milk.
33041026	9	54	theme	milk	1448:1451	arg1	technologies					1464:1475	camel milk processing technologies	1442:1475	camel milk processing technologies	1442:1475	Our results could provide the basis for the potential industrial application of camel milk processing technologies.
33041026	5	55	dep	did	815:817	arg1	as					812:813	as	812:813	as	812:813	Analysis of 16S rRNA amplicon sequences demonstrated that the composition of the intestinal microbiota of mice fed different heat-treated camel milks changed, as did the production of short-chain fatty acids as determined by gas chromatography-mass spectrometry.
33041026	6	56	theme	microbial	994:1002	arg1	diversity					1004:1012	microbial diversity	994:1012	microbial diversity of camel milk	994:1026	High temperature/short time treatment had similar effects to UHT treatment on microbial diversity of camel milk; however, the low temperature/long time treatment had different effects.
33041026	1	57	theme	milk	251:254	arg1	properties					237:246	the organoleptic and nutritional properties	204:246	the organoleptic and nutritional properties of milk	204:254	Heat treatment is the most common method used to make milk safe; however, it leads to changes in the organoleptic and nutritional properties of milk.
33041026	9	58	theme	camel	1442:1446	arg1	technologies					1464:1475	camel milk processing technologies	1442:1475	camel milk processing technologies	1442:1475	Our results could provide the basis for the potential industrial application of camel milk processing technologies.
33041026	6	59	contain	had	954:956	arg1	treatment					944:952	High temperature/short time treatment	916:952	High temperature/short time treatment	916:952	High temperature/short time treatment had similar effects to UHT treatment on microbial diversity of camel milk; however, the low temperature/long time treatment had different effects.
33041026	6	59	contain	had	954:956	arg2	effects					966:972	similar effects	958:972	similar effects to UHT treatment on microbial diversity of camel milk	958:1026	High temperature/short time treatment had similar effects to UHT treatment on microbial diversity of camel milk; however, the low temperature/long time treatment had different effects.
33041026	3	60	theme	camel	425:429	arg1	milk					431:434	camel milk	425:434	camel milk	425:434	The results showed that the nutrient composition of camel milk could be influenced by heat treatment.
33041026	5	61	theme	intestinal	734:743	arg1	microbiota					745:754	the intestinal microbiota	730:754	the intestinal microbiota of mice fed different heat-treated camel milks	730:801	Analysis of 16S rRNA amplicon sequences demonstrated that the composition of the intestinal microbiota of mice fed different heat-treated camel milks changed, as did the production of short-chain fatty acids as determined by gas chromatography-mass spectrometry.
33041026	4	62	theme	Ultra-high-temperature	475:496	arg1	treatment					498:506	Ultra-high-temperature treatment	475:506	Ultra-high-temperature treatment of samples	475:517	Ultra-high-temperature treatment of samples significantly reduced levels of camel milk proteins, vitamin C, and lactose, but did not significantly alter the amino acids content.
33041026	7	63	theme	genus	1189:1193	arg1	level					1195:1199	the genus level	1185:1199	the genus level	1185:1199	In addition, higher-temperature treatments changed the abundance of key bacteria at the genus level.
33041026	7	64	theme	bacteria	1173:1180	arg1	abundance					1156:1164	the abundance	1152:1164	the abundance of key bacteria at the genus level	1152:1199	In addition, higher-temperature treatments changed the abundance of key bacteria at the genus level.
33041026	3	65	theme	milk	431:434	arg1	composition					410:420	the nutrient composition	397:420	the nutrient composition of camel milk	397:434	The results showed that the nutrient composition of camel milk could be influenced by heat treatment.
33041026	7	66	theme	higher-temperature	1114:1131	arg1	treatments					1133:1142	higher-temperature treatments	1114:1142	higher-temperature treatments	1114:1142	In addition, higher-temperature treatments changed the abundance of key bacteria at the genus level.
33041026	8	67	theme	genera	1354:1359	arg1	proliferation					1322:1334	the proliferation	1318:1334	the proliferation of some probiotic genera	1318:1359	These results demonstrated that different heat treatments not only resulted in some nutrient loss, but also changed the proliferation of some probiotic genera.
33041026	8	68	theme	probiotic	1344:1352	arg1	genera					1354:1359	some probiotic genera	1339:1359	some probiotic genera	1339:1359	These results demonstrated that different heat treatments not only resulted in some nutrient loss, but also changed the proliferation of some probiotic genera.
33041026	4	69	theme	proteins	562:569	arg1	levels					541:546	levels	541:546	levels of camel milk proteins, vitamin C, and lactose	541:593	Ultra-high-temperature treatment of samples significantly reduced levels of camel milk proteins, vitamin C, and lactose, but did not significantly alter the amino acids content.
33041026	6	70	theme	camel	1017:1021	arg1	milk					1023:1026	camel milk	1017:1026	camel milk	1017:1026	High temperature/short time treatment had similar effects to UHT treatment on microbial diversity of camel milk; however, the low temperature/long time treatment had different effects.
33041026	1	71	theme	Heat	107:110	arg1	method					141:146	the most common method	125:146	the most common method used to make milk safe	125:169	Heat treatment is the most common method used to make milk safe; however, it leads to changes in the organoleptic and nutritional properties of milk.
33041026	1	71	theme	Heat	107:110	arg1	treatment					112:120	Heat treatment	107:120	Heat treatment	107:120	Heat treatment is the most common method used to make milk safe; however, it leads to changes in the organoleptic and nutritional properties of milk.
33041026	4	72	theme	milk	557:560	arg1	proteins					562:569	camel milk proteins	551:569	camel milk proteins	551:569	Ultra-high-temperature treatment of samples significantly reduced levels of camel milk proteins, vitamin C, and lactose, but did not significantly alter the amino acids content.
33041026	5	73	theme	sequences	683:691	arg1	Analysis					653:660	Analysis	653:660	Analysis of 16S rRNA amplicon sequences	653:691	Analysis of 16S rRNA amplicon sequences demonstrated that the composition of the intestinal microbiota of mice fed different heat-treated camel milks changed, as did the production of short-chain fatty acids as determined by gas chromatography-mass spectrometry.
33041026	6	74	theme	temperature/long	1046:1061	arg1	treatment					1068:1076	the low temperature/long time treatment	1038:1076	the low temperature/long time treatment	1038:1076	High temperature/short time treatment had similar effects to UHT treatment on microbial diversity of camel milk; however, the low temperature/long time treatment had different effects.
33041026	8	75	theme	heat	1244:1247	arg1	treatments					1249:1258	different heat treatments	1234:1258	different heat treatments	1234:1258	These results demonstrated that different heat treatments not only resulted in some nutrient loss, but also changed the proliferation of some probiotic genera.
33041026	5	76	theme	different	768:776	arg1	milks					797:801	different heat-treated camel milks	768:801	different heat-treated camel milks	768:801	Analysis of 16S rRNA amplicon sequences demonstrated that the composition of the intestinal microbiota of mice fed different heat-treated camel milks changed, as did the production of short-chain fatty acids as determined by gas chromatography-mass spectrometry.
33041026	2	77	theme	different	304:312	arg1	treatments					319:328	different heat treatments	304:328	different heat treatments	304:328	This study aimed to investigate the effects of different heat treatments on nutrients and microbiota of camel milk.
33041026	4	78	theme	vitamin	572:578	arg1	C					580:580	vitamin C	572:580	vitamin C	572:580	Ultra-high-temperature treatment of samples significantly reduced levels of camel milk proteins, vitamin C, and lactose, but did not significantly alter the amino acids content.
33041026	6	79	theme	time	1063:1066	arg1	treatment					1068:1076	the low temperature/long time treatment	1038:1076	the low temperature/long time treatment	1038:1076	High temperature/short time treatment had similar effects to UHT treatment on microbial diversity of camel milk; however, the low temperature/long time treatment had different effects.
33041026	5	80	theme	microbiota	745:754	arg1	composition					715:725	the composition	711:725	the composition of the intestinal microbiota of mice fed different heat-treated camel milks	711:801	Analysis of 16S rRNA amplicon sequences demonstrated that the composition of the intestinal microbiota of mice fed different heat-treated camel milks changed, as did the production of short-chain fatty acids as determined by gas chromatography-mass spectrometry.
33041026	5	81	theme	16S	665:667	arg1	sequences					683:691	16S rRNA amplicon sequences	665:691	16S rRNA amplicon sequences	665:691	Analysis of 16S rRNA amplicon sequences demonstrated that the composition of the intestinal microbiota of mice fed different heat-treated camel milks changed, as did the production of short-chain fatty acids as determined by gas chromatography-mass spectrometry.
32569972	0	0	theme	L	95:95	arg1	petals					119:124	Clitoria ternatea L. (butterfly pea) blue petals	77:124	Clitoria ternatea L. (butterfly pea) blue petals	77:124	Phenolic composition by UHPLC-Q-TOF-MS/MS and stability of anthocyanins from Clitoria ternatea L. (butterfly pea) blue petals.
32569972	1	1	theme	blue	286:289	arg1	petals					291:296	C. ternatea blue petals	274:296	C. ternatea blue petals	274:296	The aim of the present study was to evaluate the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light in the presence and absence of fructooligosaccharides.
32569972	6	2	theme	blue	1103:1106	arg1	petals					1108:1113	C. ternatea blue petals	1091:1113	C. ternatea blue petals	1091:1113	The data show the technological potential of aqueous extract of C. ternatea blue petals as a natural colourant in a functional beverage model system.
32569972	0	3	theme	ternatea	86:93	arg1	L					95:95	Clitoria ternatea L	77:95	Clitoria ternatea L. (butterfly pea) blue petals	77:124	Phenolic composition by UHPLC-Q-TOF-MS/MS and stability of anthocyanins from Clitoria ternatea L. (butterfly pea) blue petals.
32569972	0	3	theme	ternatea	86:93	arg1	pea					109:111	butterfly pea	99:111	butterfly pea	99:111	Phenolic composition by UHPLC-Q-TOF-MS/MS and stability of anthocyanins from Clitoria ternatea L. (butterfly pea) blue petals.
32569972	1	4	theme	petals	291:296	arg1	extracts					262:269	partially purified (PPE) extracts	237:269	partially purified (PPE) extracts	237:269	The aim of the present study was to evaluate the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light in the presence and absence of fructooligosaccharides.
32569972	1	4	theme	petals	291:296	arg1	PPE					257:259	PPE	257:259	PPE	257:259	The aim of the present study was to evaluate the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light in the presence and absence of fructooligosaccharides.
32569972	1	4	theme	petals	291:296	arg1	extracts					218:225	crude lyophilized extracts	200:225	crude lyophilized extracts (CLE)	200:231	The aim of the present study was to evaluate the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light in the presence and absence of fructooligosaccharides.
32569972	1	4	theme	petals	291:296	arg1	CLE					228:230	CLE	228:230	CLE	228:230	The aim of the present study was to evaluate the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light in the presence and absence of fructooligosaccharides.
32569972	4	5	theme	higher	855:860	arg1	energy					848:853	activation energy	837:853	activation energy higher than 99 kJ/mol	837:875	The aqueous extracts at pH 3.6 and 5.4 exhibited thermal stability with the presence and absence of fructooligosaccharides with activation energy higher than 99 kJ/mol.
32569972	5	6	theme	fructooligosaccharides	894:915	arg1	addition					882:889	The addition	878:889	The addition of fructooligosaccharides in the extracts at pH 5.4 exposed to light	878:958	The addition of fructooligosaccharides in the extracts at pH 5.4 exposed to light provided a protective effect against anthocyanin photodegradation.
32569972	2	7	theme	CLE	493:495	arg1	extracts					505:512	CLE and PPE extracts	493:512	CLE and PPE extracts	493:512	Twelve compounds were tentatively identified by UHPLC-Q-TOF-MS/MS in CLE and PPE extracts.
32569972	4	8	from	5.4	744:746	arg1	extracts					721:728	The aqueous extracts	709:728	The aqueous extracts at pH 3.6 and 5.4	709:746	The aqueous extracts at pH 3.6 and 5.4 exhibited thermal stability with the presence and absence of fructooligosaccharides with activation energy higher than 99 kJ/mol.
32569972	4	9	with	fructooligosaccharides	809:830	arg1	energy					848:853	activation energy	837:853	activation energy higher than 99 kJ/mol	837:875	The aqueous extracts at pH 3.6 and 5.4 exhibited thermal stability with the presence and absence of fructooligosaccharides with activation energy higher than 99 kJ/mol.
32569972	6	10	theme	model	1163:1167	arg1	system					1169:1174	a functional beverage model system	1141:1174	a functional beverage model system	1141:1174	The data show the technological potential of aqueous extract of C. ternatea blue petals as a natural colourant in a functional beverage model system.
32569972	3	11	theme	antioxidant	662:672	arg1	activity					674:681	antioxidant activity	662:681	antioxidant activity against the DPPH radical	662:706	In direct/reverse spectrophotometric titration, anthocyanins showed colour changes between pH 2.25 to 10.20, and colour reversibility, maintaining antioxidant activity against the DPPH radical.
32569972	5	12	from	addition	882:889	arg1	extracts					924:931	the extracts	920:931	the extracts at pH 5.4 exposed to light	920:958	The addition of fructooligosaccharides in the extracts at pH 5.4 exposed to light provided a protective effect against anthocyanin photodegradation.
32569972	1	13	theme	present	142:148	arg1	study					150:154	the present study	138:154	the present study	138:154	The aim of the present study was to evaluate the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light in the presence and absence of fructooligosaccharides.
32569972	0	14	theme	butterfly	99:107	arg1	L					95:95	Clitoria ternatea L	77:95	Clitoria ternatea L. (butterfly pea) blue petals	77:124	Phenolic composition by UHPLC-Q-TOF-MS/MS and stability of anthocyanins from Clitoria ternatea L. (butterfly pea) blue petals.
32569972	0	14	theme	butterfly	99:107	arg1	pea					109:111	butterfly pea	99:111	butterfly pea	99:111	Phenolic composition by UHPLC-Q-TOF-MS/MS and stability of anthocyanins from Clitoria ternatea L. (butterfly pea) blue petals.
32569972	3	15	theme	DPPH	695:698	arg1	radical					700:706	the DPPH radical	691:706	the DPPH radical	691:706	In direct/reverse spectrophotometric titration, anthocyanins showed colour changes between pH 2.25 to 10.20, and colour reversibility, maintaining antioxidant activity against the DPPH radical.
32569972	4	16	theme	fructooligosaccharides	809:830	arg1	absence					798:804	absence	798:804	absence	798:804	The aqueous extracts at pH 3.6 and 5.4 exhibited thermal stability with the presence and absence of fructooligosaccharides with activation energy higher than 99 kJ/mol.
32569972	4	16	theme	fructooligosaccharides	809:830	arg1	presence					785:792	presence	785:792	presence	785:792	The aqueous extracts at pH 3.6 and 5.4 exhibited thermal stability with the presence and absence of fructooligosaccharides with activation energy higher than 99 kJ/mol.
32569972	4	17	from	pH	733:734	arg1	extracts					721:728	The aqueous extracts	709:728	The aqueous extracts at pH 3.6 and 5.4	709:746	The aqueous extracts at pH 3.6 and 5.4 exhibited thermal stability with the presence and absence of fructooligosaccharides with activation energy higher than 99 kJ/mol.
32569972	1	18	theme	crude	200:204	arg1	CLE					228:230	CLE	228:230	CLE	228:230	The aim of the present study was to evaluate the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light in the presence and absence of fructooligosaccharides.
32569972	1	18	theme	crude	200:204	arg1	extracts					218:225	crude lyophilized extracts	200:225	crude lyophilized extracts (CLE)	200:231	The aim of the present study was to evaluate the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light in the presence and absence of fructooligosaccharides.
32569972	6	19	theme	ternatea	1094:1101	arg1	petals					1108:1113	C. ternatea blue petals	1091:1113	C. ternatea blue petals	1091:1113	The data show the technological potential of aqueous extract of C. ternatea blue petals as a natural colourant in a functional beverage model system.
32569972	1	20	theme	study	150:154	arg1	aim					131:133	The aim	127:133	The aim of the present study	127:154	The aim of the present study was to evaluate the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light in the presence and absence of fructooligosaccharides.
32569972	1	21	theme	anthocyanin	313:323	arg1	stability					325:333	the anthocyanin stability	309:333	the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light	172:367	The aim of the present study was to evaluate the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light in the presence and absence of fructooligosaccharides.
32569972	1	22	theme	fructooligosaccharides	400:421	arg1	absence					389:395	absence	389:395	absence	389:395	The aim of the present study was to evaluate the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light in the presence and absence of fructooligosaccharides.
32569972	1	22	theme	fructooligosaccharides	400:421	arg1	presence					376:383	presence	376:383	presence	376:383	The aim of the present study was to evaluate the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light in the presence and absence of fructooligosaccharides.
32569972	3	23	theme	spectrophotometric	533:550	arg1	titration					552:560	direct/reverse spectrophotometric titration	518:560	direct/reverse spectrophotometric titration	518:560	In direct/reverse spectrophotometric titration, anthocyanins showed colour changes between pH 2.25 to 10.20, and colour reversibility, maintaining antioxidant activity against the DPPH radical.
32569972	6	24	theme	extract	1080:1086	arg1	potential					1059:1067	the technological potential	1041:1067	the technological potential of aqueous extract of C. ternatea blue petals as a natural colourant in a functional beverage model system	1041:1174	The data show the technological potential of aqueous extract of C. ternatea blue petals as a natural colourant in a functional beverage model system.
32569972	0	25	theme	Phenolic	0:7	arg1	composition					9:19	Phenolic composition	0:19	Phenolic composition by UHPLC-Q-TOF-MS/MS and stability of anthocyanins from Clitoria ternatea L. (butterfly pea) blue petals.	0:125	Phenolic composition by UHPLC-Q-TOF-MS/MS and stability of anthocyanins from Clitoria ternatea L. (butterfly pea) blue petals.
32569972	4	26	theme	activation	837:846	arg1	energy					848:853	activation energy	837:853	activation energy higher than 99 kJ/mol	837:875	The aqueous extracts at pH 3.6 and 5.4 exhibited thermal stability with the presence and absence of fructooligosaccharides with activation energy higher than 99 kJ/mol.
32569972	4	27	theme	aqueous	713:719	arg1	extracts					721:728	The aqueous extracts	709:728	The aqueous extracts at pH 3.6 and 5.4	709:746	The aqueous extracts at pH 3.6 and 5.4 exhibited thermal stability with the presence and absence of fructooligosaccharides with activation energy higher than 99 kJ/mol.
32569972	6	28	theme	aqueous	1072:1078	arg1	extract					1080:1086	aqueous extract	1072:1086	aqueous extract of C. ternatea blue petals	1072:1113	The data show the technological potential of aqueous extract of C. ternatea blue petals as a natural colourant in a functional beverage model system.
32569972	1	29	theme	purified	247:254	arg1	extracts					262:269	partially purified (PPE) extracts	237:269	partially purified (PPE) extracts	237:269	The aim of the present study was to evaluate the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light in the presence and absence of fructooligosaccharides.
32569972	1	29	theme	purified	247:254	arg1	PPE					257:259	PPE	257:259	PPE	257:259	The aim of the present study was to evaluate the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light in the presence and absence of fructooligosaccharides.
32569972	0	30	theme	blue	114:117	arg1	petals					119:124	Clitoria ternatea L. (butterfly pea) blue petals	77:124	Clitoria ternatea L. (butterfly pea) blue petals	77:124	Phenolic composition by UHPLC-Q-TOF-MS/MS and stability of anthocyanins from Clitoria ternatea L. (butterfly pea) blue petals.
32569972	6	31	theme	technological	1045:1057	arg1	potential					1059:1067	the technological potential	1041:1067	the technological potential of aqueous extract of C. ternatea blue petals as a natural colourant in a functional beverage model system	1041:1174	The data show the technological potential of aqueous extract of C. ternatea blue petals as a natural colourant in a functional beverage model system.
32569972	6	32	theme	beverage	1154:1161	arg1	system					1169:1174	a functional beverage model system	1141:1174	a functional beverage model system	1141:1174	The data show the technological potential of aqueous extract of C. ternatea blue petals as a natural colourant in a functional beverage model system.
32569972	3	33	theme	direct/reverse	518:531	arg1	titration					552:560	direct/reverse spectrophotometric titration	518:560	direct/reverse spectrophotometric titration	518:560	In direct/reverse spectrophotometric titration, anthocyanins showed colour changes between pH 2.25 to 10.20, and colour reversibility, maintaining antioxidant activity against the DPPH radical.
32569972	2	34	theme	PPE	501:503	arg1	extracts					505:512	CLE and PPE extracts	493:512	CLE and PPE extracts	493:512	Twelve compounds were tentatively identified by UHPLC-Q-TOF-MS/MS in CLE and PPE extracts.
32569972	3	35	dep	pH	606:607	arg1	to					614:615	to	614:615	to	614:615	In direct/reverse spectrophotometric titration, anthocyanins showed colour changes between pH 2.25 to 10.20, and colour reversibility, maintaining antioxidant activity against the DPPH radical.
32569972	1	36	theme	phenolic	176:183	arg1	composition					185:195	the phenolic composition	172:195	the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light	172:367	The aim of the present study was to evaluate the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light in the presence and absence of fructooligosaccharides.
32569972	6	37	theme	C.	1091:1092	arg1	petals					1108:1113	C. ternatea blue petals	1091:1113	C. ternatea blue petals	1091:1113	The data show the technological potential of aqueous extract of C. ternatea blue petals as a natural colourant in a functional beverage model system.
32569972	3	38	theme	colour	583:588	arg1	changes					590:596	colour changes	583:596	colour changes between pH 2.25 to 10.20	583:621	In direct/reverse spectrophotometric titration, anthocyanins showed colour changes between pH 2.25 to 10.20, and colour reversibility, maintaining antioxidant activity against the DPPH radical.
32569972	4	39	dep	presence	785:792	arg1	the					781:783	the	781:783	the	781:783	The aqueous extracts at pH 3.6 and 5.4 exhibited thermal stability with the presence and absence of fructooligosaccharides with activation energy higher than 99 kJ/mol.
32569972	0	40	theme	anthocyanins	59:70	arg1	stability					46:54	stability	46:54	stability	46:54	Phenolic composition by UHPLC-Q-TOF-MS/MS and stability of anthocyanins from Clitoria ternatea L. (butterfly pea) blue petals.
32569972	0	40	theme	anthocyanins	59:70	arg1	UHPLC-Q-TOF-MS/MS					24:40	UHPLC-Q-TOF-MS/MS	24:40	UHPLC-Q-TOF-MS/MS	24:40	Phenolic composition by UHPLC-Q-TOF-MS/MS and stability of anthocyanins from Clitoria ternatea L. (butterfly pea) blue petals.
32569972	1	41	theme	extracts	262:269	arg1	stability					325:333	the anthocyanin stability	309:333	the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light	172:367	The aim of the present study was to evaluate the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light in the presence and absence of fructooligosaccharides.
32569972	1	41	theme	extracts	262:269	arg1	composition					185:195	the phenolic composition	172:195	the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light	172:367	The aim of the present study was to evaluate the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light in the presence and absence of fructooligosaccharides.
32569972	6	42	from	colourant	1128:1136	arg1	system					1169:1174	a functional beverage model system	1141:1174	a functional beverage model system	1141:1174	The data show the technological potential of aqueous extract of C. ternatea blue petals as a natural colourant in a functional beverage model system.
32569972	1	43	theme	C.	274:275	arg1	petals					291:296	C. ternatea blue petals	274:296	C. ternatea blue petals	274:296	The aim of the present study was to evaluate the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light in the presence and absence of fructooligosaccharides.
32569972	5	44	from	extracts	924:931	arg1	addition					882:889	The addition	878:889	The addition of fructooligosaccharides in the extracts at pH 5.4 exposed to light	878:958	The addition of fructooligosaccharides in the extracts at pH 5.4 exposed to light provided a protective effect against anthocyanin photodegradation.
32569972	5	45	from	fructooligosaccharides	894:915	arg1	pH					936:937	pH 5.4	936:941	pH 5.4	936:941	The addition of fructooligosaccharides in the extracts at pH 5.4 exposed to light provided a protective effect against anthocyanin photodegradation.
32569972	5	45	from	fructooligosaccharides	894:915	arg1	extracts					924:931	the extracts	920:931	the extracts at pH 5.4 exposed to light	920:958	The addition of fructooligosaccharides in the extracts at pH 5.4 exposed to light provided a protective effect against anthocyanin photodegradation.
32569972	6	46	theme	natural	1120:1126	arg1	colourant					1128:1136	a natural colourant	1118:1136	a natural colourant in a functional beverage model system	1118:1174	The data show the technological potential of aqueous extract of C. ternatea blue petals as a natural colourant in a functional beverage model system.
32569972	6	47	theme	functional	1143:1152	arg1	system					1169:1174	a functional beverage model system	1141:1174	a functional beverage model system	1141:1174	The data show the technological potential of aqueous extract of C. ternatea blue petals as a natural colourant in a functional beverage model system.
32569972	5	48	theme	protective	971:980	arg1	effect					982:987	a protective effect	969:987	a protective effect against anthocyanin photodegradation	969:1024	The addition of fructooligosaccharides in the extracts at pH 5.4 exposed to light provided a protective effect against anthocyanin photodegradation.
32569972	3	49	theme	colour	628:633	arg1	reversibility					635:647	colour reversibility	628:647	colour reversibility	628:647	In direct/reverse spectrophotometric titration, anthocyanins showed colour changes between pH 2.25 to 10.20, and colour reversibility, maintaining antioxidant activity against the DPPH radical.
32569972	4	50	theme	thermal	758:764	arg1	stability					766:774	thermal stability	758:774	thermal stability	758:774	The aqueous extracts at pH 3.6 and 5.4 exhibited thermal stability with the presence and absence of fructooligosaccharides with activation energy higher than 99 kJ/mol.
32569972	5	51	from	pH	936:937	arg1	fructooligosaccharides					894:915	fructooligosaccharides	894:915	fructooligosaccharides in the extracts at pH 5.4 exposed to light	894:958	The addition of fructooligosaccharides in the extracts at pH 5.4 exposed to light provided a protective effect against anthocyanin photodegradation.
32569972	5	51	from	pH	936:937	arg1	extracts					924:931	the extracts	920:931	the extracts at pH 5.4 exposed to light	920:958	The addition of fructooligosaccharides in the extracts at pH 5.4 exposed to light provided a protective effect against anthocyanin photodegradation.
32569972	0	52	from	petals	119:124	arg1	stability					46:54	stability	46:54	stability	46:54	Phenolic composition by UHPLC-Q-TOF-MS/MS and stability of anthocyanins from Clitoria ternatea L. (butterfly pea) blue petals.
32569972	0	52	from	petals	119:124	arg1	UHPLC-Q-TOF-MS/MS					24:40	UHPLC-Q-TOF-MS/MS	24:40	UHPLC-Q-TOF-MS/MS	24:40	Phenolic composition by UHPLC-Q-TOF-MS/MS and stability of anthocyanins from Clitoria ternatea L. (butterfly pea) blue petals.
32569972	0	53	theme	Clitoria	77:84	arg1	L					95:95	Clitoria ternatea L	77:95	Clitoria ternatea L. (butterfly pea) blue petals	77:124	Phenolic composition by UHPLC-Q-TOF-MS/MS and stability of anthocyanins from Clitoria ternatea L. (butterfly pea) blue petals.
32569972	0	53	theme	Clitoria	77:84	arg1	pea					109:111	butterfly pea	99:111	butterfly pea	99:111	Phenolic composition by UHPLC-Q-TOF-MS/MS and stability of anthocyanins from Clitoria ternatea L. (butterfly pea) blue petals.
32569972	5	54	theme	anthocyanin	997:1007	arg1	photodegradation					1009:1024	anthocyanin photodegradation	997:1024	anthocyanin photodegradation	997:1024	The addition of fructooligosaccharides in the extracts at pH 5.4 exposed to light provided a protective effect against anthocyanin photodegradation.
32569972	1	55	dep	presence	376:383	arg1	the					372:374	the	372:374	the	372:374	The aim of the present study was to evaluate the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light in the presence and absence of fructooligosaccharides.
32569972	1	56	theme	lyophilized	206:216	arg1	CLE					228:230	CLE	228:230	CLE	228:230	The aim of the present study was to evaluate the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light in the presence and absence of fructooligosaccharides.
32569972	1	56	theme	lyophilized	206:216	arg1	extracts					218:225	crude lyophilized extracts	200:225	crude lyophilized extracts (CLE)	200:231	The aim of the present study was to evaluate the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light in the presence and absence of fructooligosaccharides.
32569972	1	57	theme	ternatea	277:284	arg1	petals					291:296	C. ternatea blue petals	274:296	C. ternatea blue petals	274:296	The aim of the present study was to evaluate the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light in the presence and absence of fructooligosaccharides.
32569972	6	58	theme	petals	1108:1113	arg1	extract					1080:1086	aqueous extract	1072:1086	aqueous extract of C. ternatea blue petals	1072:1113	The data show the technological potential of aqueous extract of C. ternatea blue petals as a natural colourant in a functional beverage model system.
32569972	1	59	theme	extracts	218:225	arg1	stability					325:333	the anthocyanin stability	309:333	the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light	172:367	The aim of the present study was to evaluate the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light in the presence and absence of fructooligosaccharides.
32569972	1	59	theme	extracts	218:225	arg1	composition					185:195	the phenolic composition	172:195	the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light	172:367	The aim of the present study was to evaluate the phenolic composition of crude lyophilized extracts (CLE) and partially purified (PPE) extracts of C. ternatea blue petals as well as the anthocyanin stability against pH, temperature and light in the presence and absence of fructooligosaccharides.
32493884	3	0	theme	pathogenetic	501:512	arg1	phenotype					514:522	the pathogenetic phenotype	497:522	the pathogenetic phenotype of steatohepatitis in female DKO mice	497:560	However, the pathogenetic phenotype of steatohepatitis in female DKO mice remains unknown.
32493884	10	1	from	features	1529:1536	arg1	mice					1567:1570	female mice	1560:1570	female mice	1560:1570	Low-grade inflammatory hit in the liver under in vivo conditions of high estradiol may be attributable to the milder pathological features of steatohepatitis in female mice.
32493884	4	2	theme	steatohepatitis	601:615	arg1	changes					590:596	Phenotypic changes	579:596	Phenotypic changes of steatohepatitis in DKO mice	579:627	Phenotypic changes of steatohepatitis in DKO mice were compared in terms of gender differences.
32493884	3	3	from	phenotype	514:522	arg1	mice					557:560	female DKO mice	546:560	female DKO mice	546:560	However, the pathogenetic phenotype of steatohepatitis in female DKO mice remains unknown.
32493884	3	4	theme	DKO	553:555	arg1	mice					557:560	female DKO mice	546:560	female DKO mice	546:560	However, the pathogenetic phenotype of steatohepatitis in female DKO mice remains unknown.
32493884	10	5	from	steatohepatitis	1541:1555	arg1	mice					1567:1570	female mice	1560:1570	female mice	1560:1570	Low-grade inflammatory hit in the liver under in vivo conditions of high estradiol may be attributable to the milder pathological features of steatohepatitis in female mice.
32493884	4	6	theme	differences	662:672	arg1	terms					646:650	terms	646:650	terms of gender differences	646:672	Phenotypic changes of steatohepatitis in DKO mice were compared in terms of gender differences.
32493884	9	7	theme	steatohepatitis	1370:1384	arg1	development					1355:1365	the development	1351:1365	the development of steatohepatitis in DKO mice	1351:1396	Gender differences were observed for the development of steatohepatitis in DKO mice.
32493884	6	8	theme	Serum	862:866	arg1	estradiol					868:876	Serum estradiol	862:876	Serum estradiol	862:876	Serum estradiol was higher in female than male mice, with levels increasing up to 30 weeks of age before decreasing until 50 weeks of age (corresponding to the post-menopausal period).
32493884	2	9	from	obesity	362:368	arg1	cells					481:485	Kupffer cells	473:485	Kupffer cells	473:485	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells.
32493884	9	10	theme	DKO	1389:1391	arg1	mice					1393:1396	DKO mice	1389:1396	DKO mice	1389:1396	Gender differences were observed for the development of steatohepatitis in DKO mice.
32493884	2	11	from	overload	371:378	arg1	cells					481:485	Kupffer cells	473:485	Kupffer cells	473:485	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells.
32493884	10	12	theme	milder	1509:1514	arg1	features					1529:1536	the milder pathological features	1505:1536	the milder pathological features of steatohepatitis in female mice	1505:1570	Low-grade inflammatory hit in the liver under in vivo conditions of high estradiol may be attributable to the milder pathological features of steatohepatitis in female mice.
32493884	5	13	theme	DKO	704:706	arg1	mice					715:718	DKO female mice	704:718	DKO female mice	704:718	Compared with DKO male mice, DKO female mice exhibited later onset of steatohepatitis with obesity after 30 weeks of age, as well as milder severity of hepatic inflammation and fibrosis.
32493884	7	14	from	signaling	1126:1134	arg1	liver					1143:1147	the liver	1139:1147	the liver	1139:1147	Fecal and serum LPS were lower in female mice than male mice, and inflammatory signaling in the liver was attenuated in female compared with male mice.
32493884	10	15	theme	female	1560:1565	arg1	mice					1567:1570	female mice	1560:1570	female mice	1560:1570	Low-grade inflammatory hit in the liver under in vivo conditions of high estradiol may be attributable to the milder pathological features of steatohepatitis in female mice.
32493884	2	16	theme	E2-related	239:248	arg1	p62					260:262	p62 and Nrf2	260:271	p62	260:262	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells.
32493884	2	16	theme	E2-related	239:248	arg1	factor-2					250:257	nuclear factor E2-related factor-2	224:257	nuclear factor E2-related factor-2 (p62 and Nrf2)	224:272	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells.
32493884	2	16	theme	E2-related	239:248	arg1	Nrf2					268:271	p62 and Nrf2	260:271	Nrf2	268:271	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells.
32493884	5	17	theme	hepatic	827:833	arg1	inflammation					835:846	hepatic inflammation	827:846	hepatic inflammation	827:846	Compared with DKO male mice, DKO female mice exhibited later onset of steatohepatitis with obesity after 30 weeks of age, as well as milder severity of hepatic inflammation and fibrosis.
32493884	10	18	theme	steatohepatitis	1541:1555	arg1	features					1529:1536	the milder pathological features	1505:1536	the milder pathological features of steatohepatitis in female mice	1505:1570	Low-grade inflammatory hit in the liver under in vivo conditions of high estradiol may be attributable to the milder pathological features of steatohepatitis in female mice.
32493884	5	19	theme	DKO	689:691	arg1	mice					698:701	DKO male mice	689:701	DKO male mice	689:701	Compared with DKO male mice, DKO female mice exhibited later onset of steatohepatitis with obesity after 30 weeks of age, as well as milder severity of hepatic inflammation and fibrosis.
32493884	2	20	theme	nuclear	224:230	arg1	p62					260:262	p62 and Nrf2	260:271	p62	260:262	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells.
32493884	2	20	theme	nuclear	224:230	arg1	factor-2					250:257	nuclear factor E2-related factor-2	224:257	nuclear factor E2-related factor-2 (p62 and Nrf2)	224:272	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells.
32493884	2	20	theme	nuclear	224:230	arg1	Nrf2					268:271	p62 and Nrf2	260:271	Nrf2	268:271	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells.
32493884	3	21	theme	female	546:551	arg1	mice					557:560	female DKO mice	546:560	female DKO mice	546:560	However, the pathogenetic phenotype of steatohepatitis in female DKO mice remains unknown.
32493884	10	22	from	hit	1422:1424	arg1	liver					1433:1437	the liver	1429:1437	the liver	1429:1437	Low-grade inflammatory hit in the liver under in vivo conditions of high estradiol may be attributable to the milder pathological features of steatohepatitis in female mice.
32493884	5	23	with	steatohepatitis	745:759	arg1	obesity					766:772	obesity	766:772	obesity	766:772	Compared with DKO male mice, DKO female mice exhibited later onset of steatohepatitis with obesity after 30 weeks of age, as well as milder severity of hepatic inflammation and fibrosis.
32493884	8	24	theme	microbiota	1258:1267	arg1	different					1288:1296	different	1288:1296	different	1288:1296	Correlating with LPS levels, the composition of intestinal microbiota in female mice was different from male mice.
32493884	8	24	theme	microbiota	1258:1267	arg1	composition					1232:1242	the composition	1228:1242	the composition of intestinal microbiota in female mice	1228:1282	Correlating with LPS levels, the composition of intestinal microbiota in female mice was different from male mice.
32493884	0	25	theme	double-knockout	75:89	arg1	mice					91:94	p62/Sqstm1 and Nrf2 double-knockout mice	55:94	p62/Sqstm1 and Nrf2 double-knockout mice	55:94	Gender difference in development of steatohepatitis in p62/Sqstm1 and Nrf2 double-knockout mice.
32493884	1	26	theme	nonalcoholic	167:178	arg1	NASH					197:200	NASH	197:200	NASH	197:200	Gender and menopause influence the severity and development manner of nonalcoholic steatohepatitis (NASH).
32493884	1	26	theme	nonalcoholic	167:178	arg1	steatohepatitis					180:194	nonalcoholic steatohepatitis	167:194	nonalcoholic steatohepatitis (NASH)	167:201	Gender and menopause influence the severity and development manner of nonalcoholic steatohepatitis (NASH).
32493884	6	27	theme	age	956:958	arg1	weeks					947:951	up to 30 weeks	938:951	up to 30 weeks of age	938:958	Serum estradiol was higher in female than male mice, with levels increasing up to 30 weeks of age before decreasing until 50 weeks of age (corresponding to the post-menopausal period).
32493884	1	28	dep	severity	132:139	arg1	manner					157:162	manner	157:162	manner	157:162	Gender and menopause influence the severity and development manner of nonalcoholic steatohepatitis (NASH).
32493884	1	28	dep	severity	132:139	arg1	the					128:130	the	128:130	the	128:130	Gender and menopause influence the severity and development manner of nonalcoholic steatohepatitis (NASH).
32493884	5	29	theme	later	730:734	arg1	onset					736:740	later onset	730:740	later onset of steatohepatitis with obesity after 30 weeks of age	730:794	Compared with DKO male mice, DKO female mice exhibited later onset of steatohepatitis with obesity after 30 weeks of age, as well as milder severity of hepatic inflammation and fibrosis.
32493884	10	30	theme	inflammatory	1409:1420	arg1	hit					1422:1424	Low-grade inflammatory hit	1399:1424	Low-grade inflammatory hit in the liver under in vivo conditions of high estradiol	1399:1480	Low-grade inflammatory hit in the liver under in vivo conditions of high estradiol may be attributable to the milder pathological features of steatohepatitis in female mice.
32493884	6	31	theme	post-menopausal	1022:1036	arg1	period					1038:1043	the post-menopausal period	1018:1043	the post-menopausal period	1018:1043	Serum estradiol was higher in female than male mice, with levels increasing up to 30 weeks of age before decreasing until 50 weeks of age (corresponding to the post-menopausal period).
32493884	0	32	theme	Nrf2	70:73	arg1	mice					91:94	p62/Sqstm1 and Nrf2 double-knockout mice	55:94	p62/Sqstm1 and Nrf2 double-knockout mice	55:94	Gender difference in development of steatohepatitis in p62/Sqstm1 and Nrf2 double-knockout mice.
32493884	0	33	theme	Gender	0:5	arg1	difference					7:16	Gender difference	0:16	Gender difference in development of steatohepatitis in p62/Sqstm1 and Nrf2 double-knockout mice	0:94	Gender difference in development of steatohepatitis in p62/Sqstm1 and Nrf2 double-knockout mice.
32493884	9	34	from	development	1355:1365	arg1	mice					1393:1396	DKO mice	1389:1396	DKO mice	1389:1396	Gender differences were observed for the development of steatohepatitis in DKO mice.
32493884	2	35	theme	Kupffer	473:479	arg1	cells					481:485	Kupffer cells	473:485	Kupffer cells	473:485	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells.
32493884	1	36	theme	steatohepatitis	180:194	arg1	severity					132:139	severity	132:139	severity	132:139	Gender and menopause influence the severity and development manner of nonalcoholic steatohepatitis (NASH).
32493884	1	36	theme	steatohepatitis	180:194	arg1	development					145:155	development	145:155	development	145:155	Gender and menopause influence the severity and development manner of nonalcoholic steatohepatitis (NASH).
32493884	2	37	theme	response	461:468	arg1	overload					371:378	overload	371:378	overload of lipopolysaccharide (LPS) into the liver	371:421	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells.
32493884	2	37	theme	response	461:468	arg1	potentiation					428:439	potentiation	428:439	potentiation of the inflammatory response in Kupffer cells	428:485	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells.
32493884	2	37	theme	response	461:468	arg1	obesity					362:368	hyperphagia-induced obesity	342:368	hyperphagia-induced obesity	342:368	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells.
32493884	2	38	theme	hyperphagia-induced	342:360	arg1	obesity					362:368	hyperphagia-induced obesity	342:368	hyperphagia-induced obesity	342:368	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells.
32493884	5	39	theme	age	792:794	arg1	weeks					783:787	30 weeks	780:787	30 weeks of age	780:794	Compared with DKO male mice, DKO female mice exhibited later onset of steatohepatitis with obesity after 30 weeks of age, as well as milder severity of hepatic inflammation and fibrosis.
32493884	10	40	theme	in	1445:1446	arg1	conditions					1453:1462	in vivo conditions	1445:1462	in vivo conditions of high estradiol	1445:1480	Low-grade inflammatory hit in the liver under in vivo conditions of high estradiol may be attributable to the milder pathological features of steatohepatitis in female mice.
32493884	2	41	theme	p62/Sqstm1	209:218	arg1	mice					296:299	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice	204:299	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice	204:299	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells.
32493884	6	42	theme	male	904:907	arg1	mice					909:912	male mice	904:912	male mice	904:912	Serum estradiol was higher in female than male mice, with levels increasing up to 30 weeks of age before decreasing until 50 weeks of age (corresponding to the post-menopausal period).
32493884	7	43	theme	male	1188:1191	arg1	mice					1193:1196	male mice	1188:1196	male mice	1188:1196	Fecal and serum LPS were lower in female mice than male mice, and inflammatory signaling in the liver was attenuated in female compared with male mice.
32493884	10	44	theme	high	1467:1470	arg1	estradiol					1472:1480	high estradiol	1467:1480	high estradiol	1467:1480	Low-grade inflammatory hit in the liver under in vivo conditions of high estradiol may be attributable to the milder pathological features of steatohepatitis in female mice.
32493884	4	45	theme	Phenotypic	579:588	arg1	changes					590:596	Phenotypic changes	579:596	Phenotypic changes of steatohepatitis in DKO mice	579:627	Phenotypic changes of steatohepatitis in DKO mice were compared in terms of gender differences.
32493884	7	46	theme	serum	1057:1061	arg1	LPS					1063:1065	Fecal and serum LPS	1047:1065	LPS	1063:1065	Fecal and serum LPS were lower in female mice than male mice, and inflammatory signaling in the liver was attenuated in female compared with male mice.
32493884	2	47	theme	DKO	291:293	arg1	mice					296:299	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice	204:299	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice	204:299	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells.
32493884	7	48	theme	Fecal	1047:1051	arg1	LPS					1063:1065	Fecal and serum LPS	1047:1065	LPS	1063:1065	Fecal and serum LPS were lower in female mice than male mice, and inflammatory signaling in the liver was attenuated in female compared with male mice.
32493884	2	49	theme	double-knockout	274:288	arg1	mice					296:299	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice	204:299	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice	204:299	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells.
32493884	5	50	theme	inflammation	835:846	arg1	onset					736:740	later onset	730:740	later onset of steatohepatitis with obesity after 30 weeks of age	730:794	Compared with DKO male mice, DKO female mice exhibited later onset of steatohepatitis with obesity after 30 weeks of age, as well as milder severity of hepatic inflammation and fibrosis.
32493884	5	50	theme	inflammation	835:846	arg1	severity					815:822	milder severity	808:822	milder severity of hepatic inflammation and fibrosis	808:859	Compared with DKO male mice, DKO female mice exhibited later onset of steatohepatitis with obesity after 30 weeks of age, as well as milder severity of hepatic inflammation and fibrosis.
32493884	7	51	theme	female	1081:1086	arg1	mice					1088:1091	female mice	1081:1091	female mice	1081:1091	Fecal and serum LPS were lower in female mice than male mice, and inflammatory signaling in the liver was attenuated in female compared with male mice.
32493884	10	52	from	mice	1567:1570	arg1	features					1529:1536	the milder pathological features	1505:1536	the milder pathological features of steatohepatitis in female mice	1505:1570	Low-grade inflammatory hit in the liver under in vivo conditions of high estradiol may be attributable to the milder pathological features of steatohepatitis in female mice.
32493884	4	53	theme	gender	655:660	arg1	differences					662:672	gender differences	655:672	gender differences	655:672	Phenotypic changes of steatohepatitis in DKO mice were compared in terms of gender differences.
32493884	2	54	theme	severe	309:314	arg1	steatohepatitis					316:330	severe steatohepatitis	309:330	severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells	309:485	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells.
32493884	5	55	theme	fibrosis	852:859	arg1	onset					736:740	later onset	730:740	later onset of steatohepatitis with obesity after 30 weeks of age	730:794	Compared with DKO male mice, DKO female mice exhibited later onset of steatohepatitis with obesity after 30 weeks of age, as well as milder severity of hepatic inflammation and fibrosis.
32493884	5	55	theme	fibrosis	852:859	arg1	severity					815:822	milder severity	808:822	milder severity of hepatic inflammation and fibrosis	808:859	Compared with DKO male mice, DKO female mice exhibited later onset of steatohepatitis with obesity after 30 weeks of age, as well as milder severity of hepatic inflammation and fibrosis.
32493884	10	56	theme	pathological	1516:1527	arg1	features					1529:1536	the milder pathological features	1505:1536	the milder pathological features of steatohepatitis in female mice	1505:1570	Low-grade inflammatory hit in the liver under in vivo conditions of high estradiol may be attributable to the milder pathological features of steatohepatitis in female mice.
32493884	10	57	dep	in	1445:1446	arg1	vivo					1448:1451	vivo	1448:1451	vivo	1448:1451	Low-grade inflammatory hit in the liver under in vivo conditions of high estradiol may be attributable to the milder pathological features of steatohepatitis in female mice.
32493884	5	58	theme	milder	808:813	arg1	severity					815:822	milder severity	808:822	milder severity of hepatic inflammation and fibrosis	808:859	Compared with DKO male mice, DKO female mice exhibited later onset of steatohepatitis with obesity after 30 weeks of age, as well as milder severity of hepatic inflammation and fibrosis.
32493884	5	59	theme	female	708:713	arg1	mice					715:718	DKO female mice	704:718	DKO female mice	704:718	Compared with DKO male mice, DKO female mice exhibited later onset of steatohepatitis with obesity after 30 weeks of age, as well as milder severity of hepatic inflammation and fibrosis.
32493884	2	60	theme	factor-2	250:257	arg1	mice					296:299	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice	204:299	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice	204:299	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells.
32493884	2	61	theme	factor	232:237	arg1	p62					260:262	p62 and Nrf2	260:271	p62	260:262	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells.
32493884	2	61	theme	factor	232:237	arg1	factor-2					250:257	nuclear factor E2-related factor-2	224:257	nuclear factor E2-related factor-2 (p62 and Nrf2)	224:272	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells.
32493884	2	61	theme	factor	232:237	arg1	Nrf2					268:271	p62 and Nrf2	260:271	Nrf2	268:271	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells.
32493884	8	62	theme	LPS	1216:1218	arg1	levels					1220:1225	LPS levels	1216:1225	LPS levels	1216:1225	Correlating with LPS levels, the composition of intestinal microbiota in female mice was different from male mice.
32493884	5	63	theme	male	693:696	arg1	mice					698:701	DKO male mice	689:701	DKO male mice	689:701	Compared with DKO male mice, DKO female mice exhibited later onset of steatohepatitis with obesity after 30 weeks of age, as well as milder severity of hepatic inflammation and fibrosis.
32493884	6	64	dep	30	944:945	arg1	to					941:942	to	941:942	to	941:942	Serum estradiol was higher in female than male mice, with levels increasing up to 30 weeks of age before decreasing until 50 weeks of age (corresponding to the post-menopausal period).
32493884	8	65	theme	female	1272:1277	arg1	mice					1279:1282	female mice	1272:1282	female mice	1272:1282	Correlating with LPS levels, the composition of intestinal microbiota in female mice was different from male mice.
32493884	5	66	theme	steatohepatitis	745:759	arg1	onset					736:740	later onset	730:740	later onset of steatohepatitis with obesity after 30 weeks of age	730:794	Compared with DKO male mice, DKO female mice exhibited later onset of steatohepatitis with obesity after 30 weeks of age, as well as milder severity of hepatic inflammation and fibrosis.
32493884	5	66	theme	steatohepatitis	745:759	arg1	severity					815:822	milder severity	808:822	milder severity of hepatic inflammation and fibrosis	808:859	Compared with DKO male mice, DKO female mice exhibited later onset of steatohepatitis with obesity after 30 weeks of age, as well as milder severity of hepatic inflammation and fibrosis.
32493884	2	67	from	potentiation	428:439	arg1	cells					481:485	Kupffer cells	473:485	Kupffer cells	473:485	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells.
32493884	0	68	from	difference	7:16	arg1	development					21:31	development	21:31	development of steatohepatitis	21:50	Gender difference in development of steatohepatitis in p62/Sqstm1 and Nrf2 double-knockout mice.
32493884	0	68	from	difference	7:16	arg1	mice					91:94	p62/Sqstm1 and Nrf2 double-knockout mice	55:94	p62/Sqstm1 and Nrf2 double-knockout mice	55:94	Gender difference in development of steatohepatitis in p62/Sqstm1 and Nrf2 double-knockout mice.
32493884	8	69	theme	intestinal	1247:1256	arg1	microbiota					1258:1267	intestinal microbiota	1247:1267	intestinal microbiota	1247:1267	Correlating with LPS levels, the composition of intestinal microbiota in female mice was different from male mice.
32493884	7	70	theme	inflammatory	1113:1124	arg1	signaling					1126:1134	inflammatory signaling	1113:1134	inflammatory signaling in the liver	1113:1147	Fecal and serum LPS were lower in female mice than male mice, and inflammatory signaling in the liver was attenuated in female compared with male mice.
32493884	10	71	theme	Low-grade	1399:1407	arg1	hit					1422:1424	Low-grade inflammatory hit	1399:1424	Low-grade inflammatory hit in the liver under in vivo conditions of high estradiol	1399:1480	Low-grade inflammatory hit in the liver under in vivo conditions of high estradiol may be attributable to the milder pathological features of steatohepatitis in female mice.
32493884	4	72	from	changes	590:596	arg1	mice					624:627	DKO mice	620:627	DKO mice	620:627	Phenotypic changes of steatohepatitis in DKO mice were compared in terms of gender differences.
32493884	3	73	theme	steatohepatitis	527:541	arg1	phenotype					514:522	the pathogenetic phenotype	497:522	the pathogenetic phenotype of steatohepatitis in female DKO mice	497:560	However, the pathogenetic phenotype of steatohepatitis in female DKO mice remains unknown.
32493884	2	74	theme	lipopolysaccharide	383:400	arg1	overload					371:378	overload	371:378	overload of lipopolysaccharide (LPS) into the liver	371:421	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells.
32493884	2	74	theme	lipopolysaccharide	383:400	arg1	potentiation					428:439	potentiation	428:439	potentiation of the inflammatory response in Kupffer cells	428:485	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells.
32493884	2	74	theme	lipopolysaccharide	383:400	arg1	obesity					362:368	hyperphagia-induced obesity	342:368	hyperphagia-induced obesity	342:368	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells.
32493884	7	75	theme	male	1098:1101	arg1	mice					1103:1106	male mice	1098:1106	male mice	1098:1106	Fecal and serum LPS were lower in female mice than male mice, and inflammatory signaling in the liver was attenuated in female compared with male mice.
32493884	8	76	from	composition	1232:1242	arg1	mice					1279:1282	female mice	1272:1282	female mice	1272:1282	Correlating with LPS levels, the composition of intestinal microbiota in female mice was different from male mice.
32493884	8	77	theme	male	1303:1306	arg1	mice					1308:1311	male mice	1303:1311	male mice	1303:1311	Correlating with LPS levels, the composition of intestinal microbiota in female mice was different from male mice.
32493884	0	78	theme	steatohepatitis	36:50	arg1	development					21:31	development	21:31	development of steatohepatitis	21:50	Gender difference in development of steatohepatitis in p62/Sqstm1 and Nrf2 double-knockout mice.
32493884	6	79	theme	age	996:998	arg1	weeks					987:991	50 weeks	984:991	50 weeks of age (corresponding to the post-menopausal period)	984:1044	Serum estradiol was higher in female than male mice, with levels increasing up to 30 weeks of age before decreasing until 50 weeks of age (corresponding to the post-menopausal period).
32493884	0	80	theme	p62/Sqstm1	55:64	arg1	mice					91:94	p62/Sqstm1 and Nrf2 double-knockout mice	55:94	p62/Sqstm1 and Nrf2 double-knockout mice	55:94	Gender difference in development of steatohepatitis in p62/Sqstm1 and Nrf2 double-knockout mice.
32493884	2	81	theme	inflammatory	448:459	arg1	response					461:468	the inflammatory response	444:468	the inflammatory response	444:468	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells.
32493884	4	82	theme	DKO	620:622	arg1	mice					624:627	DKO mice	620:627	DKO mice	620:627	Phenotypic changes of steatohepatitis in DKO mice were compared in terms of gender differences.
32493884	2	83	theme	Male	204:207	arg1	p62/Sqstm1					209:218	Male p62/Sqstm1	204:218	Male p62/Sqstm1	204:218	Male p62/Sqstm1 and nuclear factor E2-related factor-2 (p62 and Nrf2) double-knockout (DKO) mice exhibit severe steatohepatitis caused by hyperphagia-induced obesity, overload of lipopolysaccharide (LPS) into the liver, and potentiation of the inflammatory response in Kupffer cells.
32493884	9	84	theme	Gender	1314:1319	arg1	differences					1321:1331	Gender differences	1314:1331	Gender differences	1314:1331	Gender differences were observed for the development of steatohepatitis in DKO mice.
32493884	10	85	theme	estradiol	1472:1480	arg1	conditions					1453:1462	in vivo conditions	1445:1462	in vivo conditions of high estradiol	1445:1480	Low-grade inflammatory hit in the liver under in vivo conditions of high estradiol may be attributable to the milder pathological features of steatohepatitis in female mice.
32493884	8	86	from	mice	1308:1311	arg1	composition					1232:1242	the composition	1228:1242	the composition of intestinal microbiota in female mice	1228:1282	Correlating with LPS levels, the composition of intestinal microbiota in female mice was different from male mice.
32493884	8	86	from	mice	1308:1311	arg1	different					1288:1296	different	1288:1296	different	1288:1296	Correlating with LPS levels, the composition of intestinal microbiota in female mice was different from male mice.
34174305	4	0	theme	hydrogels	701:709	arg1	behaviors					679:687	the swelling and rheological behaviors	650:687	the swelling and rheological behaviors of the HECS hydrogels	650:709	The amount of crosslinker had great effects on the swelling and rheological behaviors of the HECS hydrogels.
34174305	1	1	theme	matrix	194:199	arg1	characteristics					161:175	the characteristics	157:175	the characteristics of extracellular matrix (ECM) in breast tumors in terms of their structure and composition	157:266	Polysaccharide hydrogels are promising candidate matrices for recapitulating the characteristics of extracellular matrix (ECM) in breast tumors in terms of their structure and composition.
34174305	6	2	theme	MMP-2	980:984	arg1	levels					970:975	expression levels	959:975	expression levels of MMP-2, TGF-β and VEGF in comparison to two-dimension cultured cells	959:1046	Importantly, the cells displayed enhanced migrative capability and upregulated expression levels of MMP-2, TGF-β and VEGF in comparison to two-dimension cultured cells.
34174305	1	3	from	matrix	194:199	arg1	tumors					217:222	breast tumors	210:222	breast tumors in terms of their structure and composition	210:266	Polysaccharide hydrogels are promising candidate matrices for recapitulating the characteristics of extracellular matrix (ECM) in breast tumors in terms of their structure and composition.
34174305	5	4	theme	biological	746:755	arg1	assessments					757:767	in vitro biological assessments	737:767	in vitro biological assessments	737:767	Preliminary results from in vitro biological assessments confirmed that MCF-7 cells incubated within HECS hydrogels preferred to grow into three-dimensional spheroids.
34174305	4	5	theme	great	633:637	arg1	effects					639:645	great effects	633:645	great effects	633:645	The amount of crosslinker had great effects on the swelling and rheological behaviors of the HECS hydrogels.
34174305	6	6	theme	expression	959:968	arg1	levels					970:975	expression levels	959:975	expression levels of MMP-2, TGF-β and VEGF in comparison to two-dimension cultured cells	959:1046	Importantly, the cells displayed enhanced migrative capability and upregulated expression levels of MMP-2, TGF-β and VEGF in comparison to two-dimension cultured cells.
34174305	2	7	theme	ECM-mimetic	290:300	arg1	matrix					302:307	an ECM-mimetic matrix	287:307	an ECM-mimetic matrix	287:307	Herein, to obtain an ECM-mimetic matrix, hydroxyethyl chitosan (HECS) hydrogels were prepared through Schiff-base crosslinking reaction using dialdehyde hyaluronic acid as crosslinker.
34174305	1	8	theme	Polysaccharide	80:93	arg1	hydrogels					95:103	Polysaccharide hydrogels	80:103	Polysaccharide hydrogels	80:103	Polysaccharide hydrogels are promising candidate matrices for recapitulating the characteristics of extracellular matrix (ECM) in breast tumors in terms of their structure and composition.
34174305	1	8	theme	Polysaccharide	80:93	arg1	matrices					129:136	promising candidate matrices	109:136	promising candidate matrices for recapitulating the characteristics of extracellular matrix (ECM) in breast tumors in terms of their structure and composition	109:266	Polysaccharide hydrogels are promising candidate matrices for recapitulating the characteristics of extracellular matrix (ECM) in breast tumors in terms of their structure and composition.
34174305	2	9	theme	hyaluronic	422:431	arg1	acid					433:436	dialdehyde hyaluronic acid	411:436	dialdehyde hyaluronic acid	411:436	Herein, to obtain an ECM-mimetic matrix, hydroxyethyl chitosan (HECS) hydrogels were prepared through Schiff-base crosslinking reaction using dialdehyde hyaluronic acid as crosslinker.
34174305	4	10	theme	crosslinker	617:627	arg1	amount					607:612	The amount	603:612	The amount of crosslinker	603:627	The amount of crosslinker had great effects on the swelling and rheological behaviors of the HECS hydrogels.
34174305	4	10	theme	crosslinker	617:627	arg1	crosslinker					617:627	crosslinker	617:627	crosslinker	617:627	The amount of crosslinker had great effects on the swelling and rheological behaviors of the HECS hydrogels.
34174305	7	11	theme	tumor	1137:1141	arg1	models					1143:1148	breast tumor models	1130:1148	breast tumor models	1130:1148	Hence, the HECS hydrogels show great promise as a biomimetic ECM in constructing breast tumor models.
34174305	2	12	theme	dialdehyde	411:420	arg1	acid					433:436	dialdehyde hyaluronic acid	411:436	dialdehyde hyaluronic acid	411:436	Herein, to obtain an ECM-mimetic matrix, hydroxyethyl chitosan (HECS) hydrogels were prepared through Schiff-base crosslinking reaction using dialdehyde hyaluronic acid as crosslinker.
34174305	7	13	theme	breast	1130:1135	arg1	models					1143:1148	breast tumor models	1130:1148	breast tumor models	1130:1148	Hence, the HECS hydrogels show great promise as a biomimetic ECM in constructing breast tumor models.
34174305	1	14	from	characteristics	161:175	arg1	tumors					217:222	breast tumors	210:222	breast tumors in terms of their structure and composition	210:266	Polysaccharide hydrogels are promising candidate matrices for recapitulating the characteristics of extracellular matrix (ECM) in breast tumors in terms of their structure and composition.
34174305	3	15	theme	water-absorption	579:594	arg1	speed					596:600	a fast water-absorption speed	572:600	a fast water-absorption speed	572:600	The obtained HECS hydrogels displayed a highly porous structure, a stiffness comparable to that of breast tissue, and a fast water-absorption speed.
34174305	0	16	theme	Hydroxyethyl	0:11	arg1	chitosan					13:20	Hydroxyethyl chitosan	0:20	Hydroxyethyl chitosan	0:20	Hydroxyethyl chitosan hydrogels for enhancing breast cancer cell tumorigenesis.
34174305	6	17	theme	cultured	1033:1040	arg1	cells					1042:1046	two-dimension cultured cells	1019:1046	two-dimension cultured cells	1019:1046	Importantly, the cells displayed enhanced migrative capability and upregulated expression levels of MMP-2, TGF-β and VEGF in comparison to two-dimension cultured cells.
34174305	5	18	dep	hydrogels	818:826	arg1	preferred					828:836	preferred	828:836	hydrogels preferred to grow into three-dimensional spheroids	818:877	Preliminary results from in vitro biological assessments confirmed that MCF-7 cells incubated within HECS hydrogels preferred to grow into three-dimensional spheroids.
34174305	2	19	theme	chitosan	323:330	arg1	hydrogels					339:347	hydroxyethyl chitosan (HECS) hydrogels	310:347	hydroxyethyl chitosan (HECS) hydrogels	310:347	Herein, to obtain an ECM-mimetic matrix, hydroxyethyl chitosan (HECS) hydrogels were prepared through Schiff-base crosslinking reaction using dialdehyde hyaluronic acid as crosslinker.
34174305	1	20	theme	breast	210:215	arg1	tumors					217:222	breast tumors	210:222	breast tumors in terms of their structure and composition	210:266	Polysaccharide hydrogels are promising candidate matrices for recapitulating the characteristics of extracellular matrix (ECM) in breast tumors in terms of their structure and composition.
34174305	5	21	theme	three-dimensional	851:867	arg1	spheroids					869:877	three-dimensional spheroids	851:877	three-dimensional spheroids	851:877	Preliminary results from in vitro biological assessments confirmed that MCF-7 cells incubated within HECS hydrogels preferred to grow into three-dimensional spheroids.
34174305	6	22	theme	two-dimension	1019:1031	arg1	cells					1042:1046	two-dimension cultured cells	1019:1046	two-dimension cultured cells	1019:1046	Importantly, the cells displayed enhanced migrative capability and upregulated expression levels of MMP-2, TGF-β and VEGF in comparison to two-dimension cultured cells.
34174305	1	23	theme	candidate	119:127	arg1	hydrogels					95:103	Polysaccharide hydrogels	80:103	Polysaccharide hydrogels	80:103	Polysaccharide hydrogels are promising candidate matrices for recapitulating the characteristics of extracellular matrix (ECM) in breast tumors in terms of their structure and composition.
34174305	1	23	theme	candidate	119:127	arg1	matrices					129:136	promising candidate matrices	109:136	promising candidate matrices for recapitulating the characteristics of extracellular matrix (ECM) in breast tumors in terms of their structure and composition	109:266	Polysaccharide hydrogels are promising candidate matrices for recapitulating the characteristics of extracellular matrix (ECM) in breast tumors in terms of their structure and composition.
34174305	1	24	theme	promising	109:117	arg1	hydrogels					95:103	Polysaccharide hydrogels	80:103	Polysaccharide hydrogels	80:103	Polysaccharide hydrogels are promising candidate matrices for recapitulating the characteristics of extracellular matrix (ECM) in breast tumors in terms of their structure and composition.
34174305	1	24	theme	promising	109:117	arg1	matrices					129:136	promising candidate matrices	109:136	promising candidate matrices for recapitulating the characteristics of extracellular matrix (ECM) in breast tumors in terms of their structure and composition	109:266	Polysaccharide hydrogels are promising candidate matrices for recapitulating the characteristics of extracellular matrix (ECM) in breast tumors in terms of their structure and composition.
34174305	7	25	theme	biomimetic	1099:1108	arg1	ECM					1110:1112	a biomimetic ECM	1097:1112	a biomimetic ECM in constructing breast tumor models	1097:1148	Hence, the HECS hydrogels show great promise as a biomimetic ECM in constructing breast tumor models.
34174305	7	25	theme	biomimetic	1099:1108	arg1	promise					1086:1092	great promise	1080:1092	great promise	1080:1092	Hence, the HECS hydrogels show great promise as a biomimetic ECM in constructing breast tumor models.
34174305	3	26	theme	breast	553:558	arg1	tissue					560:565	breast tissue	553:565	breast tissue	553:565	The obtained HECS hydrogels displayed a highly porous structure, a stiffness comparable to that of breast tissue, and a fast water-absorption speed.
34174305	6	27	theme	migrative	922:930	arg1	capability					932:941	enhanced migrative capability	913:941	enhanced migrative capability	913:941	Importantly, the cells displayed enhanced migrative capability and upregulated expression levels of MMP-2, TGF-β and VEGF in comparison to two-dimension cultured cells.
34174305	3	28	theme	fast	574:577	arg1	speed					596:600	a fast water-absorption speed	572:600	a fast water-absorption speed	572:600	The obtained HECS hydrogels displayed a highly porous structure, a stiffness comparable to that of breast tissue, and a fast water-absorption speed.
34174305	3	29	theme	porous	501:506	arg1	structure					508:516	a highly porous structure	492:516	a highly porous structure	492:516	The obtained HECS hydrogels displayed a highly porous structure, a stiffness comparable to that of breast tissue, and a fast water-absorption speed.
34174305	0	30	theme	breast	46:51	arg1	tumorigenesis					65:77	breast cancer cell tumorigenesis	46:77	breast cancer cell tumorigenesis	46:77	Hydroxyethyl chitosan hydrogels for enhancing breast cancer cell tumorigenesis.
34174305	5	31	theme	in	737:738	arg1	assessments					757:767	in vitro biological assessments	737:767	in vitro biological assessments	737:767	Preliminary results from in vitro biological assessments confirmed that MCF-7 cells incubated within HECS hydrogels preferred to grow into three-dimensional spheroids.
34174305	6	32	theme	enhanced	913:920	arg1	capability					932:941	enhanced migrative capability	913:941	enhanced migrative capability	913:941	Importantly, the cells displayed enhanced migrative capability and upregulated expression levels of MMP-2, TGF-β and VEGF in comparison to two-dimension cultured cells.
34174305	3	33	theme	obtained	458:465	arg1	hydrogels					472:480	The obtained HECS hydrogels	454:480	The obtained HECS hydrogels	454:480	The obtained HECS hydrogels displayed a highly porous structure, a stiffness comparable to that of breast tissue, and a fast water-absorption speed.
34174305	3	34	theme	HECS	467:470	arg1	hydrogels					472:480	The obtained HECS hydrogels	454:480	The obtained HECS hydrogels	454:480	The obtained HECS hydrogels displayed a highly porous structure, a stiffness comparable to that of breast tissue, and a fast water-absorption speed.
34174305	4	35	theme	swelling	654:661	arg1	behaviors					679:687	the swelling and rheological behaviors	650:687	the swelling and rheological behaviors of the HECS hydrogels	650:709	The amount of crosslinker had great effects on the swelling and rheological behaviors of the HECS hydrogels.
34174305	0	36	theme	cell	60:63	arg1	tumorigenesis					65:77	breast cancer cell tumorigenesis	46:77	breast cancer cell tumorigenesis	46:77	Hydroxyethyl chitosan hydrogels for enhancing breast cancer cell tumorigenesis.
34174305	3	37	theme	comparable	531:540	arg1	stiffness					521:529	a stiffness	519:529	a stiffness comparable to that of breast tissue	519:565	The obtained HECS hydrogels displayed a highly porous structure, a stiffness comparable to that of breast tissue, and a fast water-absorption speed.
34174305	5	38	from	assessments	757:767	arg1	results					724:730	Preliminary results	712:730	Preliminary results from in vitro biological assessments	712:767	Preliminary results from in vitro biological assessments confirmed that MCF-7 cells incubated within HECS hydrogels preferred to grow into three-dimensional spheroids.
34174305	0	39	theme	cancer	53:58	arg1	tumorigenesis					65:77	breast cancer cell tumorigenesis	46:77	breast cancer cell tumorigenesis	46:77	Hydroxyethyl chitosan hydrogels for enhancing breast cancer cell tumorigenesis.
34174305	1	40	from	tumors	217:222	arg1	characteristics					161:175	the characteristics	157:175	the characteristics of extracellular matrix (ECM) in breast tumors in terms of their structure and composition	157:266	Polysaccharide hydrogels are promising candidate matrices for recapitulating the characteristics of extracellular matrix (ECM) in breast tumors in terms of their structure and composition.
34174305	1	40	from	tumors	217:222	arg1	terms					227:231	terms	227:231	terms of their structure and composition	227:266	Polysaccharide hydrogels are promising candidate matrices for recapitulating the characteristics of extracellular matrix (ECM) in breast tumors in terms of their structure and composition.
34174305	6	41	theme	TGF-β	987:991	arg1	levels					970:975	expression levels	959:975	expression levels of MMP-2, TGF-β and VEGF in comparison to two-dimension cultured cells	959:1046	Importantly, the cells displayed enhanced migrative capability and upregulated expression levels of MMP-2, TGF-β and VEGF in comparison to two-dimension cultured cells.
34174305	7	42	theme	great	1080:1084	arg1	ECM					1110:1112	a biomimetic ECM	1097:1112	a biomimetic ECM in constructing breast tumor models	1097:1148	Hence, the HECS hydrogels show great promise as a biomimetic ECM in constructing breast tumor models.
34174305	7	42	theme	great	1080:1084	arg1	promise					1086:1092	great promise	1080:1092	great promise	1080:1092	Hence, the HECS hydrogels show great promise as a biomimetic ECM in constructing breast tumor models.
34174305	5	43	dep	in	737:738	arg1	vitro					740:744	vitro	740:744	vitro	740:744	Preliminary results from in vitro biological assessments confirmed that MCF-7 cells incubated within HECS hydrogels preferred to grow into three-dimensional spheroids.
34174305	4	44	theme	HECS	696:699	arg1	hydrogels					701:709	the HECS hydrogels	692:709	the HECS hydrogels	692:709	The amount of crosslinker had great effects on the swelling and rheological behaviors of the HECS hydrogels.
34174305	2	45	theme	hydroxyethyl	310:321	arg1	HECS					333:336	HECS	333:336	HECS	333:336	Herein, to obtain an ECM-mimetic matrix, hydroxyethyl chitosan (HECS) hydrogels were prepared through Schiff-base crosslinking reaction using dialdehyde hyaluronic acid as crosslinker.
34174305	2	45	theme	hydroxyethyl	310:321	arg1	chitosan					323:330	hydroxyethyl chitosan	310:330	hydroxyethyl chitosan (HECS) hydrogels	310:347	Herein, to obtain an ECM-mimetic matrix, hydroxyethyl chitosan (HECS) hydrogels were prepared through Schiff-base crosslinking reaction using dialdehyde hyaluronic acid as crosslinker.
34174305	5	46	theme	Preliminary	712:722	arg1	results					724:730	Preliminary results	712:730	Preliminary results from in vitro biological assessments	712:767	Preliminary results from in vitro biological assessments confirmed that MCF-7 cells incubated within HECS hydrogels preferred to grow into three-dimensional spheroids.
34174305	1	47	theme	extracellular	180:192	arg1	ECM					202:204	ECM	202:204	ECM	202:204	Polysaccharide hydrogels are promising candidate matrices for recapitulating the characteristics of extracellular matrix (ECM) in breast tumors in terms of their structure and composition.
34174305	1	47	theme	extracellular	180:192	arg1	matrix					194:199	extracellular matrix	180:199	extracellular matrix (ECM) in breast tumors in terms of their structure and composition	180:266	Polysaccharide hydrogels are promising candidate matrices for recapitulating the characteristics of extracellular matrix (ECM) in breast tumors in terms of their structure and composition.
34174305	1	48	theme	structure	242:250	arg1	terms					227:231	terms	227:231	terms of their structure and composition	227:266	Polysaccharide hydrogels are promising candidate matrices for recapitulating the characteristics of extracellular matrix (ECM) in breast tumors in terms of their structure and composition.
34174305	5	49	theme	MCF-7	784:788	arg1	cells					790:794	MCF-7 cells	784:794	MCF-7 cells incubated within HECS	784:816	Preliminary results from in vitro biological assessments confirmed that MCF-7 cells incubated within HECS hydrogels preferred to grow into three-dimensional spheroids.
34174305	7	50	theme	HECS	1060:1063	arg1	hydrogels					1065:1073	the HECS hydrogels	1056:1073	the HECS hydrogels	1056:1073	Hence, the HECS hydrogels show great promise as a biomimetic ECM in constructing breast tumor models.
34174305	2	51	theme	crosslinking	383:394	arg1	reaction					396:403	Schiff-base crosslinking reaction	371:403	Schiff-base crosslinking reaction using dialdehyde hyaluronic acid as crosslinker	371:451	Herein, to obtain an ECM-mimetic matrix, hydroxyethyl chitosan (HECS) hydrogels were prepared through Schiff-base crosslinking reaction using dialdehyde hyaluronic acid as crosslinker.
34174305	4	52	theme	rheological	667:677	arg1	behaviors					679:687	the swelling and rheological behaviors	650:687	the swelling and rheological behaviors of the HECS hydrogels	650:709	The amount of crosslinker had great effects on the swelling and rheological behaviors of the HECS hydrogels.
34174305	6	53	from	levels	970:975	arg1	comparison					1005:1014	comparison	1005:1014	comparison to two-dimension cultured cells	1005:1046	Importantly, the cells displayed enhanced migrative capability and upregulated expression levels of MMP-2, TGF-β and VEGF in comparison to two-dimension cultured cells.
34174305	4	54	contain	had	629:631	arg1	amount					607:612	The amount	603:612	The amount of crosslinker	603:627	The amount of crosslinker had great effects on the swelling and rheological behaviors of the HECS hydrogels.
34174305	4	54	contain	had	629:631	arg2	effects					639:645	great effects	633:645	great effects	633:645	The amount of crosslinker had great effects on the swelling and rheological behaviors of the HECS hydrogels.
34174305	4	54	contain	had	629:631	arg1	crosslinker					617:627	crosslinker	617:627	crosslinker	617:627	The amount of crosslinker had great effects on the swelling and rheological behaviors of the HECS hydrogels.
34174305	2	55	theme	Schiff-base	371:381	arg1	reaction					396:403	Schiff-base crosslinking reaction	371:403	Schiff-base crosslinking reaction using dialdehyde hyaluronic acid as crosslinker	371:451	Herein, to obtain an ECM-mimetic matrix, hydroxyethyl chitosan (HECS) hydrogels were prepared through Schiff-base crosslinking reaction using dialdehyde hyaluronic acid as crosslinker.
34174305	6	56	theme	VEGF	997:1000	arg1	levels					970:975	expression levels	959:975	expression levels of MMP-2, TGF-β and VEGF in comparison to two-dimension cultured cells	959:1046	Importantly, the cells displayed enhanced migrative capability and upregulated expression levels of MMP-2, TGF-β and VEGF in comparison to two-dimension cultured cells.
34174305	1	57	theme	composition	256:266	arg1	terms					227:231	terms	227:231	terms of their structure and composition	227:266	Polysaccharide hydrogels are promising candidate matrices for recapitulating the characteristics of extracellular matrix (ECM) in breast tumors in terms of their structure and composition.
32507184	5	0	from	interaction	721:731	arg1	EC					802:803	EC	802:803	EC	802:803	It is worth noting that the FTIR analysis of the film shows that there may be an interaction between the phenolic hydroxyl group in Cap and the hydroxyl group in EC, which means that Cap has successfully participated in the film formation system.
32507184	8	1	dep	shown	1254:1258	arg1	makes					1284:1288	makes	1284:1288	makes it possible to apply these films to food packaging	1284:1339	The practical application of EC-Cap films in the protection of bell peppers has shown positive results, which makes it possible to apply these films to food packaging.
32507184	7	2	theme	pure	1082:1085	arg1	membrane					1090:1097	pure EC membrane	1082:1097	pure EC membrane	1082:1097	Compared with pure EC membrane, EC-Cap membrane has greater antibacterial activity than pure EC membrane.
32507184	7	3	contain	has	1116:1118	arg1	membrane					1107:1114	EC-Cap membrane	1100:1114	EC-Cap membrane	1100:1114	Compared with pure EC membrane, EC-Cap membrane has greater antibacterial activity than pure EC membrane.
32507184	7	3	contain	has	1116:1118	arg2	activity					1142:1149	greater antibacterial activity	1120:1149	greater antibacterial activity	1120:1149	Compared with pure EC membrane, EC-Cap membrane has greater antibacterial activity than pure EC membrane.
32507184	3	4	theme	stretchable	452:462	arg1	EC-Cap					510:515	EC-Cap	510:515	EC-Cap	510:515	Importantly, the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane (EC-Cap) is generally easy to produce and is environmentally friendly.
32507184	3	4	theme	stretchable	452:462	arg1	membrane					500:507	the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane	425:507	the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane (EC-Cap)	425:516	Importantly, the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane (EC-Cap) is generally easy to produce and is environmentally friendly.
32507184	3	4	theme	stretchable	452:462	arg1	easy					531:534	easy	531:534	easy	531:534	Importantly, the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane (EC-Cap) is generally easy to produce and is environmentally friendly.
32507184	8	5	theme	bell	1237:1240	arg1	peppers					1242:1248	bell peppers	1237:1248	bell peppers	1237:1248	The practical application of EC-Cap films in the protection of bell peppers has shown positive results, which makes it possible to apply these films to food packaging.
32507184	6	6	theme	cap-containing	902:915	arg1	film					917:920	the cap-containing film	898:920	the cap-containing film	898:920	Therefore, the cap-containing film not only exhibits a low water absorption, when the cap is appropriate, the elongation at break of the film reaches a maximum of 61.34 % ± 1.37 %.
32507184	5	7	theme	FTIR	668:671	arg1	analysis					673:680	the FTIR analysis	664:680	the FTIR analysis of the film	664:692	It is worth noting that the FTIR analysis of the film shows that there may be an interaction between the phenolic hydroxyl group in Cap and the hydroxyl group in EC, which means that Cap has successfully participated in the film formation system.
32507184	8	8	from	application	1188:1198	arg1	protection					1223:1232	the protection	1219:1232	the protection of bell peppers	1219:1248	The practical application of EC-Cap films in the protection of bell peppers has shown positive results, which makes it possible to apply these films to food packaging.
32507184	2	9	dep	properties	310:319	arg1	the					306:308	the	306:308	the	306:308	The main purpose of this work is to study the properties and specific performance of the film formed by adding capsaicin to ethyl cellulose system.
32507184	5	10	theme	film	864:867	arg1	formation					869:877	film formation	864:877	the film formation system	860:884	It is worth noting that the FTIR analysis of the film shows that there may be an interaction between the phenolic hydroxyl group in Cap and the hydroxyl group in EC, which means that Cap has successfully participated in the film formation system.
32507184	7	11	theme	greater	1120:1126	arg1	activity					1142:1149	greater antibacterial activity	1120:1149	greater antibacterial activity	1120:1149	Compared with pure EC membrane, EC-Cap membrane has greater antibacterial activity than pure EC membrane.
32507184	8	12	theme	EC-Cap	1203:1208	arg1	films					1210:1214	EC-Cap films	1203:1214	EC-Cap films in the protection of bell peppers	1203:1248	The practical application of EC-Cap films in the protection of bell peppers has shown positive results, which makes it possible to apply these films to food packaging.
32507184	7	13	theme	EC-Cap	1100:1105	arg1	membrane					1107:1114	EC-Cap membrane	1100:1114	EC-Cap membrane	1100:1114	Compared with pure EC membrane, EC-Cap membrane has greater antibacterial activity than pure EC membrane.
32507184	6	14	theme	%	1056:1056	arg1	%					1065:1065	61.34 % ± 1.37 %	1050:1065	61.34 % ± 1.37 %	1050:1065	Therefore, the cap-containing film not only exhibits a low water absorption, when the cap is appropriate, the elongation at break of the film reaches a maximum of 61.34 % ± 1.37 %.
32507184	7	15	theme	antibacterial	1128:1140	arg1	activity					1142:1149	greater antibacterial activity	1120:1149	greater antibacterial activity	1120:1149	Compared with pure EC membrane, EC-Cap membrane has greater antibacterial activity than pure EC membrane.
32507184	3	16	theme	composite	490:498	arg1	EC-Cap					510:515	EC-Cap	510:515	EC-Cap	510:515	Importantly, the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane (EC-Cap) is generally easy to produce and is environmentally friendly.
32507184	3	16	theme	composite	490:498	arg1	membrane					500:507	the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane	425:507	the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane (EC-Cap)	425:516	Importantly, the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane (EC-Cap) is generally easy to produce and is environmentally friendly.
32507184	3	16	theme	composite	490:498	arg1	easy					531:534	easy	531:534	easy	531:534	Importantly, the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane (EC-Cap) is generally easy to produce and is environmentally friendly.
32507184	2	17	theme	film	353:356	arg1	properties					310:319	properties	310:319	properties	310:319	The main purpose of this work is to study the properties and specific performance of the film formed by adding capsaicin to ethyl cellulose system.
32507184	2	17	theme	film	353:356	arg1	performance					334:344	specific performance	325:344	specific performance	325:344	The main purpose of this work is to study the properties and specific performance of the film formed by adding capsaicin to ethyl cellulose system.
32507184	1	18	theme	Pure	82:85	arg1	cannot					108:113	Pure ethyl cellulose film cannot	82:113	Pure ethyl cellulose film cannot	82:113	Pure ethyl cellulose film cannot extend the shelf life of food, and adding capsaicin as an antibacterial agent can inhibit the activity of microorganisms on the surface of the film.
32507184	5	19	theme	hydroxyl	784:791	arg1	group					793:797	the hydroxyl group	780:797	the hydroxyl group	780:797	It is worth noting that the FTIR analysis of the film shows that there may be an interaction between the phenolic hydroxyl group in Cap and the hydroxyl group in EC, which means that Cap has successfully participated in the film formation system.
32507184	7	20	theme	pure	1156:1159	arg1	membrane					1164:1171	pure EC membrane	1156:1171	pure EC membrane	1156:1171	Compared with pure EC membrane, EC-Cap membrane has greater antibacterial activity than pure EC membrane.
32507184	7	21	theme	EC	1087:1088	arg1	membrane					1090:1097	pure EC membrane	1082:1097	pure EC membrane	1082:1097	Compared with pure EC membrane, EC-Cap membrane has greater antibacterial activity than pure EC membrane.
32507184	1	22	theme	ethyl	87:91	arg1	cannot					108:113	Pure ethyl cellulose film cannot	82:113	Pure ethyl cellulose film cannot	82:113	Pure ethyl cellulose film cannot extend the shelf life of food, and adding capsaicin as an antibacterial agent can inhibit the activity of microorganisms on the surface of the film.
32507184	4	23	theme	casting	624:630	arg1	method					632:637	a casting method	622:637	a casting method	622:637	It is the first successful preparation by a casting method.
32507184	2	24	theme	cellulose	394:402	arg1	system					404:409	ethyl cellulose system	388:409	ethyl cellulose system	388:409	The main purpose of this work is to study the properties and specific performance of the film formed by adding capsaicin to ethyl cellulose system.
32507184	8	25	theme	food	1326:1329	arg1	packaging					1331:1339	food packaging	1326:1339	food packaging	1326:1339	The practical application of EC-Cap films in the protection of bell peppers has shown positive results, which makes it possible to apply these films to food packaging.
32507184	1	26	theme	cellulose	93:101	arg1	cannot					108:113	Pure ethyl cellulose film cannot	82:113	Pure ethyl cellulose film cannot	82:113	Pure ethyl cellulose film cannot extend the shelf life of food, and adding capsaicin as an antibacterial agent can inhibit the activity of microorganisms on the surface of the film.
32507184	2	27	theme	ethyl	388:392	arg1	system					404:409	ethyl cellulose system	388:409	ethyl cellulose system	388:409	The main purpose of this work is to study the properties and specific performance of the film formed by adding capsaicin to ethyl cellulose system.
32507184	1	28	theme	film	258:261	arg1	surface					243:249	the surface	239:249	the surface of the film	239:261	Pure ethyl cellulose film cannot extend the shelf life of food, and adding capsaicin as an antibacterial agent can inhibit the activity of microorganisms on the surface of the film.
32507184	8	29	theme	positive	1260:1267	arg1	results					1269:1275	positive results	1260:1275	positive results	1260:1275	The practical application of EC-Cap films in the protection of bell peppers has shown positive results, which makes it possible to apply these films to food packaging.
32507184	1	30	theme	film	103:106	arg1	cannot					108:113	Pure ethyl cellulose film cannot	82:113	Pure ethyl cellulose film cannot	82:113	Pure ethyl cellulose film cannot extend the shelf life of food, and adding capsaicin as an antibacterial agent can inhibit the activity of microorganisms on the surface of the film.
32507184	1	31	theme	antibacterial	173:185	arg1	agent					187:191	an antibacterial agent	170:191	an antibacterial agent	170:191	Pure ethyl cellulose film cannot extend the shelf life of food, and adding capsaicin as an antibacterial agent can inhibit the activity of microorganisms on the surface of the film.
32507184	1	31	theme	antibacterial	173:185	arg1	capsaicin					157:165	capsaicin	157:165	capsaicin	157:165	Pure ethyl cellulose film cannot extend the shelf life of food, and adding capsaicin as an antibacterial agent can inhibit the activity of microorganisms on the surface of the film.
32507184	5	32	theme	hydroxyl	754:761	arg1	group					763:767	the phenolic hydroxyl group	741:767	the phenolic hydroxyl group in Cap	741:774	It is worth noting that the FTIR analysis of the film shows that there may be an interaction between the phenolic hydroxyl group in Cap and the hydroxyl group in EC, which means that Cap has successfully participated in the film formation system.
32507184	6	33	theme	±	1058:1058	arg1	%					1065:1065	61.34 % ± 1.37 %	1050:1065	61.34 % ± 1.37 %	1050:1065	Therefore, the cap-containing film not only exhibits a low water absorption, when the cap is appropriate, the elongation at break of the film reaches a maximum of 61.34 % ± 1.37 %.
32507184	2	34	theme	main	268:271	arg1	purpose					273:279	The main purpose	264:279	The main purpose of this work	264:292	The main purpose of this work is to study the properties and specific performance of the film formed by adding capsaicin to ethyl cellulose system.
32507184	2	35	theme	specific	325:332	arg1	performance					334:344	specific performance	325:344	specific performance	325:344	The main purpose of this work is to study the properties and specific performance of the film formed by adding capsaicin to ethyl cellulose system.
32507184	0	36	theme	cellulose	42:50	arg1	film					52:55	ethyl cellulose film	36:55	ethyl cellulose film	36:55	Preparation and characterization of ethyl cellulose film modified with capsaicin.
32507184	7	37	theme	EC	1161:1162	arg1	membrane					1164:1171	pure EC membrane	1156:1171	pure EC membrane	1156:1171	Compared with pure EC membrane, EC-Cap membrane has greater antibacterial activity than pure EC membrane.
32507184	8	38	from	protection	1223:1232	arg1	application					1188:1198	The practical application	1174:1198	The practical application of EC-Cap films in the protection of bell peppers	1174:1248	The practical application of EC-Cap films in the protection of bell peppers has shown positive results, which makes it possible to apply these films to food packaging.
32507184	6	39	theme	water	946:950	arg1	absorption					952:961	a low water absorption	940:961	a low water absorption	940:961	Therefore, the cap-containing film not only exhibits a low water absorption, when the cap is appropriate, the elongation at break of the film reaches a maximum of 61.34 % ± 1.37 %.
32507184	8	40	theme	practical	1178:1186	arg1	application					1188:1198	The practical application	1174:1198	The practical application of EC-Cap films in the protection of bell peppers	1174:1248	The practical application of EC-Cap films in the protection of bell peppers has shown positive results, which makes it possible to apply these films to food packaging.
32507184	4	41	theme	successful	596:605	arg1	preparation					607:617	the first successful preparation	586:617	the first successful preparation by a casting method	586:637	It is the first successful preparation by a casting method.
32507184	4	41	theme	successful	596:605	arg1	It					580:581	It	580:581	It	580:581	It is the first successful preparation by a casting method.
32507184	0	42	theme	ethyl	36:40	arg1	film					52:55	ethyl cellulose film	36:55	ethyl cellulose film	36:55	Preparation and characterization of ethyl cellulose film modified with capsaicin.
32507184	5	43	theme	phenolic	745:752	arg1	group					763:767	the phenolic hydroxyl group	741:767	the phenolic hydroxyl group in Cap	741:774	It is worth noting that the FTIR analysis of the film shows that there may be an interaction between the phenolic hydroxyl group in Cap and the hydroxyl group in EC, which means that Cap has successfully participated in the film formation system.
32507184	6	44	from	break	1011:1015	arg1	elongation					997:1006	the elongation	993:1006	the elongation at break of the film	993:1027	Therefore, the cap-containing film not only exhibits a low water absorption, when the cap is appropriate, the elongation at break of the film reaches a maximum of 61.34 % ± 1.37 %.
32507184	4	45	theme	first	590:594	arg1	preparation					607:617	the first successful preparation	586:617	the first successful preparation by a casting method	586:637	It is the first successful preparation by a casting method.
32507184	4	45	theme	first	590:594	arg1	It					580:581	It	580:581	It	580:581	It is the first successful preparation by a casting method.
32507184	8	46	theme	films	1210:1214	arg1	application					1188:1198	The practical application	1174:1198	The practical application of EC-Cap films in the protection of bell peppers	1174:1248	The practical application of EC-Cap films in the protection of bell peppers has shown positive results, which makes it possible to apply these films to food packaging.
32507184	3	47	theme	soft	442:445	arg1	EC-Cap					510:515	EC-Cap	510:515	EC-Cap	510:515	Importantly, the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane (EC-Cap) is generally easy to produce and is environmentally friendly.
32507184	3	47	theme	soft	442:445	arg1	membrane					500:507	the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane	425:507	the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane (EC-Cap)	425:516	Importantly, the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane (EC-Cap) is generally easy to produce and is environmentally friendly.
32507184	3	47	theme	soft	442:445	arg1	easy					531:534	easy	531:534	easy	531:534	Importantly, the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane (EC-Cap) is generally easy to produce and is environmentally friendly.
32507184	0	48	theme	film	52:55	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of ethyl cellulose film modified with capsaicin.
32507184	0	48	theme	film	52:55	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of ethyl cellulose film modified with capsaicin.
32507184	1	49	theme	food	140:143	arg1	life					132:135	the shelf life	122:135	the shelf life of food	122:143	Pure ethyl cellulose film cannot extend the shelf life of food, and adding capsaicin as an antibacterial agent can inhibit the activity of microorganisms on the surface of the film.
32507184	5	50	from	group	763:767	arg1	Cap					772:774	Cap	772:774	Cap	772:774	It is worth noting that the FTIR analysis of the film shows that there may be an interaction between the phenolic hydroxyl group in Cap and the hydroxyl group in EC, which means that Cap has successfully participated in the film formation system.
32507184	1	51	theme	shelf	126:130	arg1	life					132:135	the shelf life	122:135	the shelf life of food	122:143	Pure ethyl cellulose film cannot extend the shelf life of food, and adding capsaicin as an antibacterial agent can inhibit the activity of microorganisms on the surface of the film.
32507184	8	52	theme	peppers	1242:1248	arg1	protection					1223:1232	the protection	1219:1232	the protection of bell peppers	1219:1248	The practical application of EC-Cap films in the protection of bell peppers has shown positive results, which makes it possible to apply these films to food packaging.
32507184	1	53	from	activity	209:216	arg1	surface					243:249	the surface	239:249	the surface of the film	239:261	Pure ethyl cellulose film cannot extend the shelf life of food, and adding capsaicin as an antibacterial agent can inhibit the activity of microorganisms on the surface of the film.
32507184	6	54	dep	Therefore	887:895	arg1	only					926:929	only	926:929	only	926:929	Therefore, the cap-containing film not only exhibits a low water absorption, when the cap is appropriate, the elongation at break of the film reaches a maximum of 61.34 % ± 1.37 %.
32507184	5	55	theme	formation	869:877	arg1	system					879:884	the film formation system	860:884	the film formation system	860:884	It is worth noting that the FTIR analysis of the film shows that there may be an interaction between the phenolic hydroxyl group in Cap and the hydroxyl group in EC, which means that Cap has successfully participated in the film formation system.
32507184	5	56	from	group	793:797	arg1	Cap					772:774	Cap	772:774	Cap	772:774	It is worth noting that the FTIR analysis of the film shows that there may be an interaction between the phenolic hydroxyl group in Cap and the hydroxyl group in EC, which means that Cap has successfully participated in the film formation system.
32507184	2	57	theme	work	289:292	arg1	purpose					273:279	The main purpose	264:279	The main purpose of this work	264:292	The main purpose of this work is to study the properties and specific performance of the film formed by adding capsaicin to ethyl cellulose system.
32507184	8	58	from	films	1210:1214	arg1	protection					1223:1232	the protection	1219:1232	the protection of bell peppers	1219:1248	The practical application of EC-Cap films in the protection of bell peppers has shown positive results, which makes it possible to apply these films to food packaging.
32507184	3	59	theme	transparent	429:439	arg1	EC-Cap					510:515	EC-Cap	510:515	EC-Cap	510:515	Importantly, the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane (EC-Cap) is generally easy to produce and is environmentally friendly.
32507184	3	59	theme	transparent	429:439	arg1	membrane					500:507	the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane	425:507	the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane (EC-Cap)	425:516	Importantly, the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane (EC-Cap) is generally easy to produce and is environmentally friendly.
32507184	3	59	theme	transparent	429:439	arg1	easy					531:534	easy	531:534	easy	531:534	Importantly, the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane (EC-Cap) is generally easy to produce and is environmentally friendly.
32507184	5	60	theme	film	689:692	arg1	analysis					673:680	the FTIR analysis	664:680	the FTIR analysis of the film	664:692	It is worth noting that the FTIR analysis of the film shows that there may be an interaction between the phenolic hydroxyl group in Cap and the hydroxyl group in EC, which means that Cap has successfully participated in the film formation system.
32507184	6	61	theme	low	942:944	arg1	absorption					952:961	a low water absorption	940:961	a low water absorption	940:961	Therefore, the cap-containing film not only exhibits a low water absorption, when the cap is appropriate, the elongation at break of the film reaches a maximum of 61.34 % ± 1.37 %.
32507184	6	62	theme	%	1065:1065	arg1	maximum					1039:1045	a maximum	1037:1045	a maximum of 61.34 % ± 1.37 %	1037:1065	Therefore, the cap-containing film not only exhibits a low water absorption, when the cap is appropriate, the elongation at break of the film reaches a maximum of 61.34 % ± 1.37 %.
32507184	3	63	theme	ethyl	464:468	arg1	EC-Cap					510:515	EC-Cap	510:515	EC-Cap	510:515	Importantly, the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane (EC-Cap) is generally easy to produce and is environmentally friendly.
32507184	3	63	theme	ethyl	464:468	arg1	membrane					500:507	the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane	425:507	the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane (EC-Cap)	425:516	Importantly, the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane (EC-Cap) is generally easy to produce and is environmentally friendly.
32507184	3	63	theme	ethyl	464:468	arg1	easy					531:534	easy	531:534	easy	531:534	Importantly, the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane (EC-Cap) is generally easy to produce and is environmentally friendly.
32507184	6	64	theme	film	1024:1027	arg1	elongation					997:1006	the elongation	993:1006	the elongation at break of the film	993:1027	Therefore, the cap-containing film not only exhibits a low water absorption, when the cap is appropriate, the elongation at break of the film reaches a maximum of 61.34 % ± 1.37 %.
32507184	3	65	theme	cellulose-capsaicin	470:488	arg1	EC-Cap					510:515	EC-Cap	510:515	EC-Cap	510:515	Importantly, the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane (EC-Cap) is generally easy to produce and is environmentally friendly.
32507184	3	65	theme	cellulose-capsaicin	470:488	arg1	membrane					500:507	the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane	425:507	the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane (EC-Cap)	425:516	Importantly, the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane (EC-Cap) is generally easy to produce and is environmentally friendly.
32507184	3	65	theme	cellulose-capsaicin	470:488	arg1	easy					531:534	easy	531:534	easy	531:534	Importantly, the transparent, soft, and stretchable ethyl cellulose-capsaicin composite membrane (EC-Cap) is generally easy to produce and is environmentally friendly.
32507184	1	66	theme	microorganisms	221:234	arg1	activity					209:216	the activity	205:216	the activity of microorganisms on the surface of the film	205:261	Pure ethyl cellulose film cannot extend the shelf life of food, and adding capsaicin as an antibacterial agent can inhibit the activity of microorganisms on the surface of the film.
34249773	10	0	theme	WT	1521:1522	arg1	CRC					1531:1533	WT female CRC and Nrf2 KO male CRC groups	1521:1561	CRC	1531:1533	However, the abundance of L. murinus was lower in WT female CRC and Nrf2 KO male CRC groups than in its controls.
34249773	14	1	theme	IL-1β	1963:1967	arg1	levels					1969:1974	IL-1β levels	1963:1974	IL-1β levels	1963:1974	In addition, B. vulgatus showed positive correlation with inflammatory markers (i.e. myeloperoxidase and IL-1β levels), tumor numbers, and high-grade adenoma, especially, developed mucosal and submucosal invasive adenocarcinoma at the distal part of the colon.
34249773	10	2	theme	male	1547:1550	arg1	groups					1556:1561	WT female CRC and Nrf2 KO male CRC groups	1521:1561	groups	1556:1561	However, the abundance of L. murinus was lower in WT female CRC and Nrf2 KO male CRC groups than in its controls.
34249773	14	3	theme	colon	2112:2116	arg1	part					2100:2103	the distal part	2089:2103	the distal part of the colon	2089:2116	In addition, B. vulgatus showed positive correlation with inflammatory markers (i.e. myeloperoxidase and IL-1β levels), tumor numbers, and high-grade adenoma, especially, developed mucosal and submucosal invasive adenocarcinoma at the distal part of the colon.
34249773	5	4	theme	alpha	764:768	arg1	diversity					770:778	higher alpha diversity	757:778	higher alpha diversity	757:778	In terms of sex differences, our results showed that the wild-type (WT) male control mice had higher alpha diversity (i.e. Chao1, Shannon, and Simpson) than the WT female control mice.
34249773	1	5	theme	carcinogenesis	164:177	arg1	frequency					97:105	The frequency	93:105	The frequency of azoxymethane/dextran sulfate sodium (AOM/DSS)-induced carcinogenesis in male mice	93:190	The frequency of azoxymethane/dextran sulfate sodium (AOM/DSS)-induced carcinogenesis in male mice is higher than that in female mice.
34249773	1	5	theme	carcinogenesis	164:177	arg1	higher					195:200	higher	195:200	higher	195:200	The frequency of azoxymethane/dextran sulfate sodium (AOM/DSS)-induced carcinogenesis in male mice is higher than that in female mice.
34249773	12	6	theme	Nrf2	1731:1734	arg1	KO					1736:1737	Nrf2 KO	1731:1737	Nrf2 KO	1731:1737	In contrast, the abundances of L. murinus and B. vulgatus were changed differently by Nrf2 KO depending on sex and CRC.
34249773	1	7	theme	male	182:185	arg1	mice					187:190	male mice	182:190	male mice	182:190	The frequency of azoxymethane/dextran sulfate sodium (AOM/DSS)-induced carcinogenesis in male mice is higher than that in female mice.
34249773	14	8	theme	inflammatory	1916:1927	arg1	markers					1929:1935	inflammatory markers	1916:1935	inflammatory markers (i.e. myeloperoxidase and IL-1β levels)	1916:1975	In addition, B. vulgatus showed positive correlation with inflammatory markers (i.e. myeloperoxidase and IL-1β levels), tumor numbers, and high-grade adenoma, especially, developed mucosal and submucosal invasive adenocarcinoma at the distal part of the colon.
34249773	5	9	theme	differences	679:689	arg1	terms					666:670	terms	666:670	terms of sex differences	666:689	In terms of sex differences, our results showed that the wild-type (WT) male control mice had higher alpha diversity (i.e. Chao1, Shannon, and Simpson) than the WT female control mice.
34249773	6	10	theme	size	888:891	arg1	results					901:907	The linear discriminant analysis effect size (LEfSe) results	848:907	The linear discriminant analysis effect size (LEfSe) results	848:907	The linear discriminant analysis effect size (LEfSe) results revealed that the abundances of Akkermansia muciniphila and Lactobacillus murinus were higher in WT male control mice than in WT female controls.
34249773	5	11	dep	wild-type	720:728	arg1	WT					731:732	WT	731:732	WT	731:732	In terms of sex differences, our results showed that the wild-type (WT) male control mice had higher alpha diversity (i.e. Chao1, Shannon, and Simpson) than the WT female control mice.
34249773	8	12	theme	CRC	1301:1303	arg1	groups					1305:1310	male CRC groups	1296:1310	male CRC groups	1296:1310	Furthermore, the abundance of A. muciniphila was higher in male CRC groups than in male controls in both WT and Nrf2 KO.
34249773	11	13	theme	muciniphila	1605:1615	arg1	abundance					1589:1597	The abundance	1585:1597	The abundance of A. muciniphila	1585:1615	The abundance of A. muciniphila was not altered by Nrf2 KO.
34249773	7	14	theme	tumorigenesis	1073:1085	arg1	terms					1058:1062	terms	1058:1062	terms of colon tumorigenesis	1058:1085	In terms of colon tumorigenesis, the alpha diversity of the male CRC group was lower than that of the male controls in both WT and Nrf2 KO, but did not show such changes in females.
34249773	6	15	theme	analysis	872:879	arg1	LEfSe					894:898	LEfSe	894:898	LEfSe	894:898	The linear discriminant analysis effect size (LEfSe) results revealed that the abundances of Akkermansia muciniphila and Lactobacillus murinus were higher in WT male control mice than in WT female controls.
34249773	6	15	theme	analysis	872:879	arg1	size					888:891	The linear discriminant analysis effect size	848:891	The linear discriminant analysis effect size (LEfSe) results	848:907	The linear discriminant analysis effect size (LEfSe) results revealed that the abundances of Akkermansia muciniphila and Lactobacillus murinus were higher in WT male control mice than in WT female controls.
34249773	4	16	dep	sequencing	593:602	arg1	samples					610:616	fecal samples	604:616	16S rRNA gene sequencing fecal samples obtained at week 16 post-AOM administration	579:660	The gut microbiome composition was determined by 16S rRNA gene sequencing fecal samples obtained at week 16 post-AOM administration.
34249773	6	17	theme	linear	852:857	arg1	analysis					872:879	The linear discriminant analysis	848:879	The linear discriminant analysis effect size (LEfSe) results	848:907	The linear discriminant analysis effect size (LEfSe) results revealed that the abundances of Akkermansia muciniphila and Lactobacillus murinus were higher in WT male control mice than in WT female controls.
34249773	8	18	theme	male	1320:1323	arg1	controls					1325:1332	male controls	1320:1332	male controls	1320:1332	Furthermore, the abundance of A. muciniphila was higher in male CRC groups than in male controls in both WT and Nrf2 KO.
34249773	10	19	theme	CRC	1552:1554	arg1	groups					1556:1561	WT female CRC and Nrf2 KO male CRC groups	1521:1561	groups	1556:1561	However, the abundance of L. murinus was lower in WT female CRC and Nrf2 KO male CRC groups than in its controls.
34249773	15	20	dep	sex	2205:2207	arg1	induction					2217:2225	induction	2217:2225	induction	2217:2225	In conclusion, Nrf2 differentially alters the gut microbiota composition depending on sex and CRC induction.
34249773	7	21	theme	such	1212:1215	arg1	changes					1217:1223	such changes	1212:1223	such changes in females	1212:1234	In terms of colon tumorigenesis, the alpha diversity of the male CRC group was lower than that of the male controls in both WT and Nrf2 KO, but did not show such changes in females.
34249773	14	22	theme	submucosal	2051:2060	arg1	adenocarcinoma					2071:2084	mucosal and submucosal invasive adenocarcinoma	2039:2084	mucosal and submucosal invasive adenocarcinoma	2039:2084	In addition, B. vulgatus showed positive correlation with inflammatory markers (i.e. myeloperoxidase and IL-1β levels), tumor numbers, and high-grade adenoma, especially, developed mucosal and submucosal invasive adenocarcinoma at the distal part of the colon.
34249773	15	23	theme	gut	2165:2167	arg1	composition					2180:2190	the gut microbiota composition	2161:2190	the gut microbiota composition	2161:2190	In conclusion, Nrf2 differentially alters the gut microbiota composition depending on sex and CRC induction.
34249773	5	24	theme	female	827:832	arg1	mice					842:845	the WT female control mice	820:845	the WT female control mice	820:845	In terms of sex differences, our results showed that the wild-type (WT) male control mice had higher alpha diversity (i.e. Chao1, Shannon, and Simpson) than the WT female control mice.
34249773	0	25	from	Changes	0:6	arg1	Composition					31:41	Microbial Community Composition	11:41	Microbial Community Composition	11:41	Changes in Microbial Community Composition Related to Sex and Colon Cancer by Nrf2 Knockout.
34249773	14	26	theme	mucosal	2039:2045	arg1	adenocarcinoma					2071:2084	mucosal and submucosal invasive adenocarcinoma	2039:2084	mucosal and submucosal invasive adenocarcinoma	2039:2084	In addition, B. vulgatus showed positive correlation with inflammatory markers (i.e. myeloperoxidase and IL-1β levels), tumor numbers, and high-grade adenoma, especially, developed mucosal and submucosal invasive adenocarcinoma at the distal part of the colon.
34249773	7	27	theme	CRC	1120:1122	arg1	group					1124:1128	the male CRC group	1111:1128	the male CRC group	1111:1128	In terms of colon tumorigenesis, the alpha diversity of the male CRC group was lower than that of the male controls in both WT and Nrf2 KO, but did not show such changes in females.
34249773	9	28	theme	vulgatus	1387:1394	arg1	higher					1400:1405	higher	1400:1405	higher	1400:1405	The abundance of Bacteroides vulgatus was higher in WT CRC groups than in WT controls in both males and females.
34249773	9	28	theme	vulgatus	1387:1394	arg1	abundance					1362:1370	The abundance	1358:1370	The abundance of Bacteroides vulgatus	1358:1394	The abundance of Bacteroides vulgatus was higher in WT CRC groups than in WT controls in both males and females.
34249773	3	29	theme	mouse	423:427	arg1	composition					444:454	mouse gut microbiome composition	423:454	mouse gut microbiome composition	423:454	This study aimed to investigate the changes in mouse gut microbiome composition based on sex, AOM/DSS-induced colorectal cancer (CRC), and Nrf2 genotype.
34249773	13	30	theme	negative	1798:1805	arg1	correlation					1807:1817	negative correlation	1798:1817	negative correlation with tumor numbers	1798:1836	Interestingly, L. murinus showed negative correlation with tumor numbers in the whole colon.
34249773	7	31	dep	WT	1179:1180	arg1	KO					1191:1192	KO	1191:1192	KO	1191:1192	In terms of colon tumorigenesis, the alpha diversity of the male CRC group was lower than that of the male controls in both WT and Nrf2 KO, but did not show such changes in females.
34249773	6	32	theme	WT	1006:1007	arg1	mice					1022:1025	WT male control mice	1006:1025	WT male control mice	1006:1025	The linear discriminant analysis effect size (LEfSe) results revealed that the abundances of Akkermansia muciniphila and Lactobacillus murinus were higher in WT male control mice than in WT female controls.
34249773	0	33	theme	Colon	62:66	arg1	Cancer					68:73	Colon Cancer	62:73	Colon Cancer	62:73	Changes in Microbial Community Composition Related to Sex and Colon Cancer by Nrf2 Knockout.
34249773	7	34	theme	alpha	1092:1096	arg1	diversity					1098:1106	the alpha diversity	1088:1106	the alpha diversity of the male CRC group	1088:1128	In terms of colon tumorigenesis, the alpha diversity of the male CRC group was lower than that of the male controls in both WT and Nrf2 KO, but did not show such changes in females.
34249773	7	34	theme	alpha	1092:1096	arg1	lower					1134:1138	lower	1134:1138	lower	1134:1138	In terms of colon tumorigenesis, the alpha diversity of the male CRC group was lower than that of the male controls in both WT and Nrf2 KO, but did not show such changes in females.
34249773	1	35	from	frequency	97:105	arg1	mice					187:190	male mice	182:190	male mice	182:190	The frequency of azoxymethane/dextran sulfate sodium (AOM/DSS)-induced carcinogenesis in male mice is higher than that in female mice.
34249773	9	36	theme	WT	1410:1411	arg1	groups					1417:1422	WT CRC groups	1410:1422	WT CRC groups	1410:1422	The abundance of Bacteroides vulgatus was higher in WT CRC groups than in WT controls in both males and females.
34249773	14	37	theme	high-grade	1997:2006	arg1	adenoma					2008:2014	high-grade adenoma	1997:2014	high-grade adenoma	1997:2014	In addition, B. vulgatus showed positive correlation with inflammatory markers (i.e. myeloperoxidase and IL-1β levels), tumor numbers, and high-grade adenoma, especially, developed mucosal and submucosal invasive adenocarcinoma at the distal part of the colon.
34249773	0	38	theme	Microbial	11:19	arg1	Composition					31:41	Microbial Community Composition	11:41	Microbial Community Composition	11:41	Changes in Microbial Community Composition Related to Sex and Colon Cancer by Nrf2 Knockout.
34249773	6	39	theme	murinus	983:989	arg1	higher					996:1001	higher	996:1001	higher	996:1001	The linear discriminant analysis effect size (LEfSe) results revealed that the abundances of Akkermansia muciniphila and Lactobacillus murinus were higher in WT male control mice than in WT female controls.
34249773	6	39	theme	murinus	983:989	arg1	abundances					927:936	the abundances	923:936	the abundances of Akkermansia muciniphila and Lactobacillus murinus	923:989	The linear discriminant analysis effect size (LEfSe) results revealed that the abundances of Akkermansia muciniphila and Lactobacillus murinus were higher in WT male control mice than in WT female controls.
34249773	4	40	theme	gut	534:536	arg1	composition					549:559	The gut microbiome composition	530:559	The gut microbiome composition	530:559	The gut microbiome composition was determined by 16S rRNA gene sequencing fecal samples obtained at week 16 post-AOM administration.
34249773	14	41	with	correlation	1899:1909	arg1	markers					1929:1935	inflammatory markers	1916:1935	inflammatory markers (i.e. myeloperoxidase and IL-1β levels)	1916:1975	In addition, B. vulgatus showed positive correlation with inflammatory markers (i.e. myeloperoxidase and IL-1β levels), tumor numbers, and high-grade adenoma, especially, developed mucosal and submucosal invasive adenocarcinoma at the distal part of the colon.
34249773	14	41	with	correlation	1899:1909	arg1	adenoma					2008:2014	high-grade adenoma	1997:2014	high-grade adenoma	1997:2014	In addition, B. vulgatus showed positive correlation with inflammatory markers (i.e. myeloperoxidase and IL-1β levels), tumor numbers, and high-grade adenoma, especially, developed mucosal and submucosal invasive adenocarcinoma at the distal part of the colon.
34249773	14	41	with	correlation	1899:1909	arg1	numbers					1984:1990	tumor numbers	1978:1990	tumor numbers	1978:1990	In addition, B. vulgatus showed positive correlation with inflammatory markers (i.e. myeloperoxidase and IL-1β levels), tumor numbers, and high-grade adenoma, especially, developed mucosal and submucosal invasive adenocarcinoma at the distal part of the colon.
34249773	3	42	theme	microbiome	433:442	arg1	composition					444:454	mouse gut microbiome composition	423:454	mouse gut microbiome composition	423:454	This study aimed to investigate the changes in mouse gut microbiome composition based on sex, AOM/DSS-induced colorectal cancer (CRC), and Nrf2 genotype.
34249773	7	43	theme	colon	1067:1071	arg1	tumorigenesis					1073:1085	colon tumorigenesis	1067:1085	colon tumorigenesis	1067:1085	In terms of colon tumorigenesis, the alpha diversity of the male CRC group was lower than that of the male controls in both WT and Nrf2 KO, but did not show such changes in females.
34249773	5	44	theme	control	740:746	arg1	mice					748:751	the wild-type (WT) male control mice	716:751	the wild-type (WT) male control mice	716:751	In terms of sex differences, our results showed that the wild-type (WT) male control mice had higher alpha diversity (i.e. Chao1, Shannon, and Simpson) than the WT female control mice.
34249773	8	45	theme	muciniphila	1270:1280	arg1	higher					1286:1291	higher	1286:1291	higher	1286:1291	Furthermore, the abundance of A. muciniphila was higher in male CRC groups than in male controls in both WT and Nrf2 KO.
34249773	8	45	theme	muciniphila	1270:1280	arg1	abundance					1254:1262	the abundance	1250:1262	the abundance of A. muciniphila	1250:1280	Furthermore, the abundance of A. muciniphila was higher in male CRC groups than in male controls in both WT and Nrf2 KO.
34249773	3	46	theme	AOM/DSS-induced	470:484	arg1	CRC					505:507	CRC	505:507	CRC	505:507	This study aimed to investigate the changes in mouse gut microbiome composition based on sex, AOM/DSS-induced colorectal cancer (CRC), and Nrf2 genotype.
34249773	3	46	theme	AOM/DSS-induced	470:484	arg1	cancer					497:502	AOM/DSS-induced colorectal cancer	470:502	AOM/DSS-induced colorectal cancer (CRC)	470:508	This study aimed to investigate the changes in mouse gut microbiome composition based on sex, AOM/DSS-induced colorectal cancer (CRC), and Nrf2 genotype.
34249773	2	47	theme	nuclear	324:330	arg1	factor					359:364	nuclear factor-erythroid 2-related factor 2	324:366	nuclear factor-erythroid 2-related factor 2 (Nrf2)	324:373	Previous studies have reported that 17β-estradiol inhibits tumorigenesis in males by modulating nuclear factor-erythroid 2-related factor 2 (Nrf2).
34249773	2	47	theme	nuclear	324:330	arg1	Nrf2					369:372	Nrf2	369:372	Nrf2	369:372	Previous studies have reported that 17β-estradiol inhibits tumorigenesis in males by modulating nuclear factor-erythroid 2-related factor 2 (Nrf2).
34249773	10	48	theme	Nrf2	1539:1542	arg1	groups					1556:1561	WT female CRC and Nrf2 KO male CRC groups	1521:1561	groups	1556:1561	However, the abundance of L. murinus was lower in WT female CRC and Nrf2 KO male CRC groups than in its controls.
34249773	6	49	theme	WT	1035:1036	arg1	controls					1045:1052	WT female controls	1035:1052	WT female controls	1035:1052	The linear discriminant analysis effect size (LEfSe) results revealed that the abundances of Akkermansia muciniphila and Lactobacillus murinus were higher in WT male control mice than in WT female controls.
34249773	4	50	theme	16S	579:581	arg1	sequencing					593:602	16S rRNA gene sequencing	579:602	16S rRNA gene sequencing fecal samples obtained at week 16 post-AOM administration	579:660	The gut microbiome composition was determined by 16S rRNA gene sequencing fecal samples obtained at week 16 post-AOM administration.
34249773	8	51	theme	male	1296:1299	arg1	groups					1305:1310	male CRC groups	1296:1310	male CRC groups	1296:1310	Furthermore, the abundance of A. muciniphila was higher in male CRC groups than in male controls in both WT and Nrf2 KO.
34249773	1	52	theme	female	215:220	arg1	mice					222:225	female mice	215:225	female mice	215:225	The frequency of azoxymethane/dextran sulfate sodium (AOM/DSS)-induced carcinogenesis in male mice is higher than that in female mice.
34249773	4	53	dep	week	630:633	arg1	administration					647:660	post-AOM administration	638:660	week 16 post-AOM administration	630:660	The gut microbiome composition was determined by 16S rRNA gene sequencing fecal samples obtained at week 16 post-AOM administration.
34249773	7	54	theme	male	1157:1160	arg1	controls					1162:1169	the male controls	1153:1169	the male controls	1153:1169	In terms of colon tumorigenesis, the alpha diversity of the male CRC group was lower than that of the male controls in both WT and Nrf2 KO, but did not show such changes in females.
34249773	4	55	theme	gene	588:591	arg1	sequencing					593:602	16S rRNA gene sequencing	579:602	16S rRNA gene sequencing fecal samples obtained at week 16 post-AOM administration	579:660	The gut microbiome composition was determined by 16S rRNA gene sequencing fecal samples obtained at week 16 post-AOM administration.
34249773	10	56	theme	female	1524:1529	arg1	CRC					1531:1533	WT female CRC and Nrf2 KO male CRC groups	1521:1561	CRC	1531:1533	However, the abundance of L. murinus was lower in WT female CRC and Nrf2 KO male CRC groups than in its controls.
34249773	6	57	theme	control	1014:1020	arg1	mice					1022:1025	WT male control mice	1006:1025	WT male control mice	1006:1025	The linear discriminant analysis effect size (LEfSe) results revealed that the abundances of Akkermansia muciniphila and Lactobacillus murinus were higher in WT male control mice than in WT female controls.
34249773	3	58	theme	Nrf2	515:518	arg1	genotype					520:527	Nrf2 genotype	515:527	Nrf2 genotype	515:527	This study aimed to investigate the changes in mouse gut microbiome composition based on sex, AOM/DSS-induced colorectal cancer (CRC), and Nrf2 genotype.
34249773	2	59	theme	2-related	349:357	arg1	factor					359:364	nuclear factor-erythroid 2-related factor 2	324:366	nuclear factor-erythroid 2-related factor 2 (Nrf2)	324:373	Previous studies have reported that 17β-estradiol inhibits tumorigenesis in males by modulating nuclear factor-erythroid 2-related factor 2 (Nrf2).
34249773	2	59	theme	2-related	349:357	arg1	Nrf2					369:372	Nrf2	369:372	Nrf2	369:372	Previous studies have reported that 17β-estradiol inhibits tumorigenesis in males by modulating nuclear factor-erythroid 2-related factor 2 (Nrf2).
34249773	10	60	theme	KO	1544:1545	arg1	groups					1556:1561	WT female CRC and Nrf2 KO male CRC groups	1521:1561	groups	1556:1561	However, the abundance of L. murinus was lower in WT female CRC and Nrf2 KO male CRC groups than in its controls.
34249773	1	61	theme	-induced	155:162	arg1	carcinogenesis					164:177	azoxymethane/dextran sulfate sodium (AOM/DSS)-induced carcinogenesis	110:177	azoxymethane/dextran sulfate sodium (AOM/DSS)-induced carcinogenesis	110:177	The frequency of azoxymethane/dextran sulfate sodium (AOM/DSS)-induced carcinogenesis in male mice is higher than that in female mice.
34249773	5	62	dep	had	753:755	arg1	Shannon					793:799	Shannon	793:799	Shannon	793:799	In terms of sex differences, our results showed that the wild-type (WT) male control mice had higher alpha diversity (i.e. Chao1, Shannon, and Simpson) than the WT female control mice.
34249773	5	62	dep	had	753:755	arg1	Simpson					806:812	Simpson	806:812	Simpson	806:812	In terms of sex differences, our results showed that the wild-type (WT) male control mice had higher alpha diversity (i.e. Chao1, Shannon, and Simpson) than the WT female control mice.
34249773	5	62	dep	had	753:755	arg1	Chao1					786:790	Chao1	786:790	Chao1	786:790	In terms of sex differences, our results showed that the wild-type (WT) male control mice had higher alpha diversity (i.e. Chao1, Shannon, and Simpson) than the WT female control mice.
34249773	4	63	theme	post-AOM	638:645	arg1	administration					647:660	post-AOM administration	638:660	week 16 post-AOM administration	630:660	The gut microbiome composition was determined by 16S rRNA gene sequencing fecal samples obtained at week 16 post-AOM administration.
34249773	5	64	theme	sex	675:677	arg1	differences					679:689	sex differences	675:689	sex differences	675:689	In terms of sex differences, our results showed that the wild-type (WT) male control mice had higher alpha diversity (i.e. Chao1, Shannon, and Simpson) than the WT female control mice.
34249773	4	65	theme	fecal	604:608	arg1	samples					610:616	fecal samples	604:616	16S rRNA gene sequencing fecal samples obtained at week 16 post-AOM administration	579:660	The gut microbiome composition was determined by 16S rRNA gene sequencing fecal samples obtained at week 16 post-AOM administration.
34249773	2	66	theme	factor-erythroid	332:347	arg1	factor					359:364	nuclear factor-erythroid 2-related factor 2	324:366	nuclear factor-erythroid 2-related factor 2 (Nrf2)	324:373	Previous studies have reported that 17β-estradiol inhibits tumorigenesis in males by modulating nuclear factor-erythroid 2-related factor 2 (Nrf2).
34249773	2	66	theme	factor-erythroid	332:347	arg1	Nrf2					369:372	Nrf2	369:372	Nrf2	369:372	Previous studies have reported that 17β-estradiol inhibits tumorigenesis in males by modulating nuclear factor-erythroid 2-related factor 2 (Nrf2).
34249773	12	67	theme	vulgatus	1694:1701	arg1	abundances					1662:1671	the abundances	1658:1671	the abundances of L. murinus and B. vulgatus	1658:1701	In contrast, the abundances of L. murinus and B. vulgatus were changed differently by Nrf2 KO depending on sex and CRC.
34249773	6	68	theme	effect	881:886	arg1	LEfSe					894:898	LEfSe	894:898	LEfSe	894:898	The linear discriminant analysis effect size (LEfSe) results revealed that the abundances of Akkermansia muciniphila and Lactobacillus murinus were higher in WT male control mice than in WT female controls.
34249773	6	68	theme	effect	881:886	arg1	size					888:891	The linear discriminant analysis effect size	848:891	The linear discriminant analysis effect size (LEfSe) results	848:907	The linear discriminant analysis effect size (LEfSe) results revealed that the abundances of Akkermansia muciniphila and Lactobacillus murinus were higher in WT male control mice than in WT female controls.
34249773	14	69	theme	positive	1890:1897	arg1	correlation					1899:1909	positive correlation	1890:1909	positive correlation with inflammatory markers (i.e. myeloperoxidase and IL-1β levels), tumor numbers, and high-grade adenoma, especially,	1890:2027	In addition, B. vulgatus showed positive correlation with inflammatory markers (i.e. myeloperoxidase and IL-1β levels), tumor numbers, and high-grade adenoma, especially, developed mucosal and submucosal invasive adenocarcinoma at the distal part of the colon.
34249773	7	70	theme	the male	1111:1118	arg1	group					1124:1128	the male CRC group	1111:1128	the male CRC group	1111:1128	In terms of colon tumorigenesis, the alpha diversity of the male CRC group was lower than that of the male controls in both WT and Nrf2 KO, but did not show such changes in females.
34249773	5	71	theme	control	834:840	arg1	mice					842:845	the WT female control mice	820:845	the WT female control mice	820:845	In terms of sex differences, our results showed that the wild-type (WT) male control mice had higher alpha diversity (i.e. Chao1, Shannon, and Simpson) than the WT female control mice.
34249773	6	72	theme	discriminant	859:870	arg1	analysis					872:879	The linear discriminant analysis	848:879	The linear discriminant analysis effect size (LEfSe) results	848:907	The linear discriminant analysis effect size (LEfSe) results revealed that the abundances of Akkermansia muciniphila and Lactobacillus murinus were higher in WT male control mice than in WT female controls.
34249773	5	73	contain	had	753:755	arg1	mice					748:751	the wild-type (WT) male control mice	716:751	the wild-type (WT) male control mice	716:751	In terms of sex differences, our results showed that the wild-type (WT) male control mice had higher alpha diversity (i.e. Chao1, Shannon, and Simpson) than the WT female control mice.
34249773	5	73	contain	had	753:755	arg2	diversity					770:778	higher alpha diversity	757:778	higher alpha diversity	757:778	In terms of sex differences, our results showed that the wild-type (WT) male control mice had higher alpha diversity (i.e. Chao1, Shannon, and Simpson) than the WT female control mice.
34249773	14	74	theme	distal	2093:2098	arg1	part					2100:2103	the distal part	2089:2103	the distal part of the colon	2089:2116	In addition, B. vulgatus showed positive correlation with inflammatory markers (i.e. myeloperoxidase and IL-1β levels), tumor numbers, and high-grade adenoma, especially, developed mucosal and submucosal invasive adenocarcinoma at the distal part of the colon.
34249773	13	75	theme	whole	1845:1849	arg1	colon					1851:1855	the whole colon	1841:1855	the whole colon	1841:1855	Interestingly, L. murinus showed negative correlation with tumor numbers in the whole colon.
34249773	13	76	with	correlation	1807:1817	arg1	numbers					1830:1836	tumor numbers	1824:1836	tumor numbers	1824:1836	Interestingly, L. murinus showed negative correlation with tumor numbers in the whole colon.
34249773	6	77	theme	muciniphila	953:963	arg1	higher					996:1001	higher	996:1001	higher	996:1001	The linear discriminant analysis effect size (LEfSe) results revealed that the abundances of Akkermansia muciniphila and Lactobacillus murinus were higher in WT male control mice than in WT female controls.
34249773	6	77	theme	muciniphila	953:963	arg1	abundances					927:936	the abundances	923:936	the abundances of Akkermansia muciniphila and Lactobacillus murinus	923:989	The linear discriminant analysis effect size (LEfSe) results revealed that the abundances of Akkermansia muciniphila and Lactobacillus murinus were higher in WT male control mice than in WT female controls.
34249773	5	78	theme	wild-type	720:728	arg1	mice					748:751	the wild-type (WT) male control mice	716:751	the wild-type (WT) male control mice	716:751	In terms of sex differences, our results showed that the wild-type (WT) male control mice had higher alpha diversity (i.e. Chao1, Shannon, and Simpson) than the WT female control mice.
34249773	2	79	theme	Previous	228:235	arg1	studies					237:243	Previous studies	228:243	Previous studies	228:243	Previous studies have reported that 17β-estradiol inhibits tumorigenesis in males by modulating nuclear factor-erythroid 2-related factor 2 (Nrf2).
34249773	14	80	dep	myeloperoxidase	1943:1957	arg1	i.e.					1938:1941	i.e.	1938:1941	i.e.	1938:1941	In addition, B. vulgatus showed positive correlation with inflammatory markers (i.e. myeloperoxidase and IL-1β levels), tumor numbers, and high-grade adenoma, especially, developed mucosal and submucosal invasive adenocarcinoma at the distal part of the colon.
34249773	14	81	theme	invasive	2062:2069	arg1	adenocarcinoma					2071:2084	mucosal and submucosal invasive adenocarcinoma	2039:2084	mucosal and submucosal invasive adenocarcinoma	2039:2084	In addition, B. vulgatus showed positive correlation with inflammatory markers (i.e. myeloperoxidase and IL-1β levels), tumor numbers, and high-grade adenoma, especially, developed mucosal and submucosal invasive adenocarcinoma at the distal part of the colon.
34249773	7	82	theme	group	1124:1128	arg1	diversity					1098:1106	the alpha diversity	1088:1106	the alpha diversity of the male CRC group	1088:1128	In terms of colon tumorigenesis, the alpha diversity of the male CRC group was lower than that of the male controls in both WT and Nrf2 KO, but did not show such changes in females.
34249773	7	82	theme	group	1124:1128	arg1	lower					1134:1138	lower	1134:1138	lower	1134:1138	In terms of colon tumorigenesis, the alpha diversity of the male CRC group was lower than that of the male controls in both WT and Nrf2 KO, but did not show such changes in females.
34249773	13	83	theme	tumor	1824:1828	arg1	numbers					1830:1836	tumor numbers	1824:1836	tumor numbers	1824:1836	Interestingly, L. murinus showed negative correlation with tumor numbers in the whole colon.
34249773	12	84	theme	murinus	1679:1685	arg1	abundances					1662:1671	the abundances	1658:1671	the abundances of L. murinus and B. vulgatus	1658:1701	In contrast, the abundances of L. murinus and B. vulgatus were changed differently by Nrf2 KO depending on sex and CRC.
34249773	5	85	theme	WT	824:825	arg1	mice					842:845	the WT female control mice	820:845	the WT female control mice	820:845	In terms of sex differences, our results showed that the wild-type (WT) male control mice had higher alpha diversity (i.e. Chao1, Shannon, and Simpson) than the WT female control mice.
34249773	2	86	from	tumorigenesis	287:299	arg1	males					304:308	males	304:308	males	304:308	Previous studies have reported that 17β-estradiol inhibits tumorigenesis in males by modulating nuclear factor-erythroid 2-related factor 2 (Nrf2).
34249773	0	87	theme	Knockout	83:90	arg1	Nrf2					78:81	Nrf2 Knockout	78:90	Nrf2 Knockout	78:90	Changes in Microbial Community Composition Related to Sex and Colon Cancer by Nrf2 Knockout.
34249773	15	88	theme	microbiota	2169:2178	arg1	composition					2180:2190	the gut microbiota composition	2161:2190	the gut microbiota composition	2161:2190	In conclusion, Nrf2 differentially alters the gut microbiota composition depending on sex and CRC induction.
34249773	9	89	theme	Bacteroides	1375:1385	arg1	vulgatus					1387:1394	Bacteroides vulgatus	1375:1394	Bacteroides vulgatus	1375:1394	The abundance of Bacteroides vulgatus was higher in WT CRC groups than in WT controls in both males and females.
34249773	7	90	from	changes	1217:1223	arg1	females					1228:1234	females	1228:1234	females	1228:1234	In terms of colon tumorigenesis, the alpha diversity of the male CRC group was lower than that of the male controls in both WT and Nrf2 KO, but did not show such changes in females.
34249773	6	91	theme	male	1009:1012	arg1	mice					1022:1025	WT male control mice	1006:1025	WT male control mice	1006:1025	The linear discriminant analysis effect size (LEfSe) results revealed that the abundances of Akkermansia muciniphila and Lactobacillus murinus were higher in WT male control mice than in WT female controls.
34249773	3	92	theme	gut	429:431	arg1	composition					444:454	mouse gut microbiome composition	423:454	mouse gut microbiome composition	423:454	This study aimed to investigate the changes in mouse gut microbiome composition based on sex, AOM/DSS-induced colorectal cancer (CRC), and Nrf2 genotype.
34249773	5	93	theme	higher	757:762	arg1	diversity					770:778	higher alpha diversity	757:778	higher alpha diversity	757:778	In terms of sex differences, our results showed that the wild-type (WT) male control mice had higher alpha diversity (i.e. Chao1, Shannon, and Simpson) than the WT female control mice.
34249773	14	94	dep	showed	1883:1888	arg1	developed					2029:2037	developed	2029:2037	showed positive correlation with inflammatory markers (i.e. myeloperoxidase and IL-1β levels), tumor numbers, and high-grade adenoma, especially, developed mucosal and submucosal invasive adenocarcinoma at the distal part of the colon	1883:2116	In addition, B. vulgatus showed positive correlation with inflammatory markers (i.e. myeloperoxidase and IL-1β levels), tumor numbers, and high-grade adenoma, especially, developed mucosal and submucosal invasive adenocarcinoma at the distal part of the colon.
34249773	0	95	theme	Community	21:29	arg1	Composition					31:41	Microbial Community Composition	11:41	Microbial Community Composition	11:41	Changes in Microbial Community Composition Related to Sex and Colon Cancer by Nrf2 Knockout.
34249773	9	96	theme	CRC	1413:1415	arg1	groups					1417:1422	WT CRC groups	1410:1422	WT CRC groups	1410:1422	The abundance of Bacteroides vulgatus was higher in WT CRC groups than in WT controls in both males and females.
34249773	4	97	theme	microbiome	538:547	arg1	composition					549:559	The gut microbiome composition	530:559	The gut microbiome composition	530:559	The gut microbiome composition was determined by 16S rRNA gene sequencing fecal samples obtained at week 16 post-AOM administration.
34249773	5	98	theme	male	735:738	arg1	mice					748:751	the wild-type (WT) male control mice	716:751	the wild-type (WT) male control mice	716:751	In terms of sex differences, our results showed that the wild-type (WT) male control mice had higher alpha diversity (i.e. Chao1, Shannon, and Simpson) than the WT female control mice.
34249773	9	99	theme	WT	1432:1433	arg1	controls					1435:1442	WT controls	1432:1442	WT controls	1432:1442	The abundance of Bacteroides vulgatus was higher in WT CRC groups than in WT controls in both males and females.
34249773	3	100	theme	colorectal	486:495	arg1	CRC					505:507	CRC	505:507	CRC	505:507	This study aimed to investigate the changes in mouse gut microbiome composition based on sex, AOM/DSS-induced colorectal cancer (CRC), and Nrf2 genotype.
34249773	3	100	theme	colorectal	486:495	arg1	cancer					497:502	AOM/DSS-induced colorectal cancer	470:502	AOM/DSS-induced colorectal cancer (CRC)	470:508	This study aimed to investigate the changes in mouse gut microbiome composition based on sex, AOM/DSS-induced colorectal cancer (CRC), and Nrf2 genotype.
34249773	6	101	theme	female	1038:1043	arg1	controls					1045:1052	WT female controls	1035:1052	WT female controls	1035:1052	The linear discriminant analysis effect size (LEfSe) results revealed that the abundances of Akkermansia muciniphila and Lactobacillus murinus were higher in WT male control mice than in WT female controls.
34249773	4	102	theme	rRNA	583:586	arg1	sequencing					593:602	16S rRNA gene sequencing	579:602	16S rRNA gene sequencing fecal samples obtained at week 16 post-AOM administration	579:660	The gut microbiome composition was determined by 16S rRNA gene sequencing fecal samples obtained at week 16 post-AOM administration.
34249773	3	103	from	changes	412:418	arg1	composition					444:454	mouse gut microbiome composition	423:454	mouse gut microbiome composition	423:454	This study aimed to investigate the changes in mouse gut microbiome composition based on sex, AOM/DSS-induced colorectal cancer (CRC), and Nrf2 genotype.
34249773	10	104	theme	murinus	1500:1506	arg1	lower					1512:1516	lower	1512:1516	lower	1512:1516	However, the abundance of L. murinus was lower in WT female CRC and Nrf2 KO male CRC groups than in its controls.
34249773	10	104	theme	murinus	1500:1506	arg1	abundance					1484:1492	the abundance	1480:1492	the abundance of L. murinus	1480:1506	However, the abundance of L. murinus was lower in WT female CRC and Nrf2 KO male CRC groups than in its controls.
34249773	14	105	theme	tumor	1978:1982	arg1	numbers					1984:1990	tumor numbers	1978:1990	tumor numbers	1978:1990	In addition, B. vulgatus showed positive correlation with inflammatory markers (i.e. myeloperoxidase and IL-1β levels), tumor numbers, and high-grade adenoma, especially, developed mucosal and submucosal invasive adenocarcinoma at the distal part of the colon.
34249773	5	106	dep	Chao1	786:790	arg1	i.e.					781:784	i.e.	781:784	i.e.	781:784	In terms of sex differences, our results showed that the wild-type (WT) male control mice had higher alpha diversity (i.e. Chao1, Shannon, and Simpson) than the WT female control mice.
34096615	0	0	theme	tree	59:62	arg1	roots					64:68	tree roots	59:68	tree roots	59:68	The size and the age of the metabolically active carbon in tree roots.
34096615	7	1	theme	girdled	1088:1094	arg1	roots					1096:1100	girdled roots	1088:1100	girdled roots	1088:1100	We estimate that over 3 months NSC in girdled roots must be replaced 5-7 times by reserves remobilized from root-external sources.
34096615	5	2	theme	root	881:884	arg1	excision					886:893	root excision	881:893	root excision	881:893	While freshly excised roots (mostly <2.9 mm in diameter) respired CO2 fixed <1 year previously, the age increased to 1.6-2.9 year within a week after root excision.
34096615	4	3	theme	bomb	603:606	arg1	radiocarbon					608:618	bomb radiocarbon	603:618	bomb radiocarbon	603:618	We used bomb radiocarbon to estimate the time elapsed since C fixation for respired CO2 , water-soluble NSC and structural α-cellulose.
34096615	8	4	theme	mixing	1189:1194	arg1	model					1196:1200	a mixing model	1187:1200	a mixing model	1187:1200	Using a mixing model and observed correlations between Δ14 C of water-soluble C and α-cellulose, we estimate ~30% of C is "active" (~5 mg C g-1 ).
34096615	0	5	from	size	4:7	arg1	roots					64:68	tree roots	59:68	tree roots	59:68	The size and the age of the metabolically active carbon in tree roots.
34096615	5	6	theme	CO2	797:799	arg1	<1 year					807:813	CO2 fixed <1 year	797:813	CO2 fixed <1 year	797:813	While freshly excised roots (mostly <2.9 mm in diameter) respired CO2 fixed <1 year previously, the age increased to 1.6-2.9 year within a week after root excision.
34096615	1	7	theme	C	116:116	arg1	age					109:111	age	109:111	age of C	109:116	Little is known about the sources and age of C respired by tree roots.
34096615	1	7	theme	C	116:116	arg1	sources					97:103	the sources	93:103	the sources	93:103	Little is known about the sources and age of C respired by tree roots.
34096615	8	8	theme	C	1259:1259	arg1	C					1240:1240	Δ14 C	1236:1240	Δ14 C of water-soluble C and α-cellulose	1236:1275	Using a mixing model and observed correlations between Δ14 C of water-soluble C and α-cellulose, we estimate ~30% of C is "active" (~5 mg C g-1 ).
34096615	6	9	theme	similar	988:994	arg1	rates					967:971	respiration rates	955:971	respiration rates	955:971	Freshly excised roots from trees girdled ~3 months ago had respiration rates and NSC stocks similar to un-girdled trees but respired older C (~1.2 year).
34096615	5	10	dep	roots	753:757	arg1	<2.9 mm					767:773	<2.9 mm	767:773	<2.9 mm	767:773	While freshly excised roots (mostly <2.9 mm in diameter) respired CO2 fixed <1 year previously, the age increased to 1.6-2.9 year within a week after root excision.
34096615	5	11	from	<2.9 mm	767:773	arg1	diameter					778:785	diameter	778:785	diameter	778:785	While freshly excised roots (mostly <2.9 mm in diameter) respired CO2 fixed <1 year previously, the age increased to 1.6-2.9 year within a week after root excision.
34096615	3	12	theme	Populus	480:486	arg1	hybrids					496:502	Populus tremula hybrids	480:502	Populus tremula hybrids	480:502	We compared the C isotope composition of water-soluble NSC and respired CO2 for aspen roots (Populus tremula hybrids) cut off from fresh C supply after stem-girdling or prolonged incubation of excised roots.
34096615	3	12	theme	Populus	480:486	arg1	roots					473:477	aspen roots	467:477	aspen roots (Populus tremula hybrids) cut off from fresh C supply after stem-girdling or prolonged incubation of excised roots	467:592	We compared the C isotope composition of water-soluble NSC and respired CO2 for aspen roots (Populus tremula hybrids) cut off from fresh C supply after stem-girdling or prolonged incubation of excised roots.
34096615	3	13	theme	aspen	467:471	arg1	hybrids					496:502	Populus tremula hybrids	480:502	Populus tremula hybrids	480:502	We compared the C isotope composition of water-soluble NSC and respired CO2 for aspen roots (Populus tremula hybrids) cut off from fresh C supply after stem-girdling or prolonged incubation of excised roots.
34096615	3	13	theme	aspen	467:471	arg1	roots					473:477	aspen roots	467:477	aspen roots (Populus tremula hybrids) cut off from fresh C supply after stem-girdling or prolonged incubation of excised roots	467:592	We compared the C isotope composition of water-soluble NSC and respired CO2 for aspen roots (Populus tremula hybrids) cut off from fresh C supply after stem-girdling or prolonged incubation of excised roots.
34096615	6	14	theme	older	1029:1033	arg1	~1.2 year					1038:1046	~1.2 year	1038:1046	~1.2 year	1038:1046	Freshly excised roots from trees girdled ~3 months ago had respiration rates and NSC stocks similar to un-girdled trees but respired older C (~1.2 year).
34096615	6	14	theme	older	1029:1033	arg1	C					1035:1035	older C	1029:1035	older C (~1.2 year)	1029:1047	Freshly excised roots from trees girdled ~3 months ago had respiration rates and NSC stocks similar to un-girdled trees but respired older C (~1.2 year).
34096615	2	15	theme	stored	334:339	arg1	pool					342:345	a "stored" pool	331:345	a "stored" pool used when fresh C supplies are limited	331:384	Previous research in stems identified two functional pools of non-structural carbohydrates (NSC): an "active" pool supplied directly from canopy photo-assimilates supporting metabolism and a "stored" pool used when fresh C supplies are limited.
34096615	2	16	from	research	151:158	arg1	stems					163:167	stems	163:167	stems	163:167	Previous research in stems identified two functional pools of non-structural carbohydrates (NSC): an "active" pool supplied directly from canopy photo-assimilates supporting metabolism and a "stored" pool used when fresh C supplies are limited.
34096615	5	17	theme	fixed	801:805	arg1	<1 year					807:813	CO2 fixed <1 year	797:813	CO2 fixed <1 year	797:813	While freshly excised roots (mostly <2.9 mm in diameter) respired CO2 fixed <1 year previously, the age increased to 1.6-2.9 year within a week after root excision.
34096615	4	18	used	used	598:601	arg2	We					595:596	We	595:596	We	595:596	We used bomb radiocarbon to estimate the time elapsed since C fixation for respired CO2 , water-soluble NSC and structural α-cellulose.
34096615	4	19	theme	respired	670:677	arg1	CO2					679:681	CO2	679:681	CO2	679:681	We used bomb radiocarbon to estimate the time elapsed since C fixation for respired CO2 , water-soluble NSC and structural α-cellulose.
34096615	0	20	from	age	17:19	arg1	roots					64:68	tree roots	59:68	tree roots	59:68	The size and the age of the metabolically active carbon in tree roots.
34096615	2	21	theme	"	250:250	arg1	pool					252:255	an "active" pool	240:255	an "active" pool supplied directly from canopy photo-assimilates supporting metabolism	240:325	Previous research in stems identified two functional pools of non-structural carbohydrates (NSC): an "active" pool supplied directly from canopy photo-assimilates supporting metabolism and a "stored" pool used when fresh C supplies are limited.
34096615	1	22	theme	tree	130:133	arg1	roots					135:139	tree roots	130:139	tree roots	130:139	Little is known about the sources and age of C respired by tree roots.
34096615	8	23	theme	C	1319:1319	arg1	"					1310:1310	"active"	1303:1310	"active" (~5 mg C g-1 )	1303:1325	Using a mixing model and observed correlations between Δ14 C of water-soluble C and α-cellulose, we estimate ~30% of C is "active" (~5 mg C g-1 ).
34096615	8	23	theme	C	1319:1319	arg1	g-1					1321:1323	~5 mg C g-1	1313:1323	~5 mg C g-1	1313:1323	Using a mixing model and observed correlations between Δ14 C of water-soluble C and α-cellulose, we estimate ~30% of C is "active" (~5 mg C g-1 ).
34096615	2	24	theme	active	244:249	arg1	pool					252:255	an "active" pool	240:255	an "active" pool supplied directly from canopy photo-assimilates supporting metabolism	240:325	Previous research in stems identified two functional pools of non-structural carbohydrates (NSC): an "active" pool supplied directly from canopy photo-assimilates supporting metabolism and a "stored" pool used when fresh C supplies are limited.
34096615	6	25	theme	respiration	955:965	arg1	rates					967:971	respiration rates	955:971	respiration rates	955:971	Freshly excised roots from trees girdled ~3 months ago had respiration rates and NSC stocks similar to un-girdled trees but respired older C (~1.2 year).
34096615	2	26	theme	C	363:363	arg1	supplies					365:372	fresh C supplies	357:372	fresh C supplies	357:372	Previous research in stems identified two functional pools of non-structural carbohydrates (NSC): an "active" pool supplied directly from canopy photo-assimilates supporting metabolism and a "stored" pool used when fresh C supplies are limited.
34096615	8	27	theme	active	1304:1309	arg1	"					1310:1310	"active"	1303:1310	"active" (~5 mg C g-1 )	1303:1325	Using a mixing model and observed correlations between Δ14 C of water-soluble C and α-cellulose, we estimate ~30% of C is "active" (~5 mg C g-1 ).
34096615	8	27	theme	active	1304:1309	arg1	g-1					1321:1323	~5 mg C g-1	1313:1323	~5 mg C g-1	1313:1323	Using a mixing model and observed correlations between Δ14 C of water-soluble C and α-cellulose, we estimate ~30% of C is "active" (~5 mg C g-1 ).
34096615	4	28	theme	C	655:655	arg1	fixation					657:664	C fixation	655:664	C fixation	655:664	We used bomb radiocarbon to estimate the time elapsed since C fixation for respired CO2 , water-soluble NSC and structural α-cellulose.
34096615	2	29	dep	identified	169:178	arg1	pool					342:345	a "stored" pool	331:345	a "stored" pool used when fresh C supplies are limited	331:384	Previous research in stems identified two functional pools of non-structural carbohydrates (NSC): an "active" pool supplied directly from canopy photo-assimilates supporting metabolism and a "stored" pool used when fresh C supplies are limited.
34096615	2	29	dep	identified	169:178	arg1	pool					252:255	an "active" pool	240:255	an "active" pool supplied directly from canopy photo-assimilates supporting metabolism	240:325	Previous research in stems identified two functional pools of non-structural carbohydrates (NSC): an "active" pool supplied directly from canopy photo-assimilates supporting metabolism and a "stored" pool used when fresh C supplies are limited.
34096615	2	30	theme	fresh	357:361	arg1	supplies					365:372	fresh C supplies	357:372	fresh C supplies	357:372	Previous research in stems identified two functional pools of non-structural carbohydrates (NSC): an "active" pool supplied directly from canopy photo-assimilates supporting metabolism and a "stored" pool used when fresh C supplies are limited.
34096615	2	31	theme	canopy	280:285	arg1	photo-assimilates					287:303	canopy photo-assimilates	280:303	canopy photo-assimilates supporting metabolism	280:325	Previous research in stems identified two functional pools of non-structural carbohydrates (NSC): an "active" pool supplied directly from canopy photo-assimilates supporting metabolism and a "stored" pool used when fresh C supplies are limited.
34096615	5	32	theme	excised	745:751	arg1	roots					753:757	freshly excised roots	737:757	freshly excised roots (mostly <2.9 mm in diameter)	737:786	While freshly excised roots (mostly <2.9 mm in diameter) respired CO2 fixed <1 year previously, the age increased to 1.6-2.9 year within a week after root excision.
34096615	3	33	theme	prolonged	556:564	arg1	incubation					566:575	stem-girdling or prolonged incubation	539:575	stem-girdling or prolonged incubation of excised roots	539:592	We compared the C isotope composition of water-soluble NSC and respired CO2 for aspen roots (Populus tremula hybrids) cut off from fresh C supply after stem-girdling or prolonged incubation of excised roots.
34096615	2	34	theme	functional	184:193	arg1	pools					195:199	two functional pools	180:199	two functional pools of non-structural carbohydrates (NSC)	180:237	Previous research in stems identified two functional pools of non-structural carbohydrates (NSC): an "active" pool supplied directly from canopy photo-assimilates supporting metabolism and a "stored" pool used when fresh C supplies are limited.
34096615	6	35	from	~3 months	937:945	arg1	roots					912:916	Freshly excised roots	896:916	Freshly excised roots from trees girdled ~3 months	896:945	Freshly excised roots from trees girdled ~3 months ago had respiration rates and NSC stocks similar to un-girdled trees but respired older C (~1.2 year).
34096615	6	36	theme	NSC	977:979	arg1	stocks					981:986	NSC stocks	977:986	NSC stocks	977:986	Freshly excised roots from trees girdled ~3 months ago had respiration rates and NSC stocks similar to un-girdled trees but respired older C (~1.2 year).
34096615	7	37	from	NSC	1081:1083	arg1	roots					1096:1100	girdled roots	1088:1100	girdled roots	1088:1100	We estimate that over 3 months NSC in girdled roots must be replaced 5-7 times by reserves remobilized from root-external sources.
34096615	4	38	theme	structural	707:716	arg1	α-cellulose					718:728	structural α-cellulose	707:728	structural α-cellulose	707:728	We used bomb radiocarbon to estimate the time elapsed since C fixation for respired CO2 , water-soluble NSC and structural α-cellulose.
34096615	2	39	theme	"	340:340	arg1	pool					342:345	a "stored" pool	331:345	a "stored" pool used when fresh C supplies are limited	331:384	Previous research in stems identified two functional pools of non-structural carbohydrates (NSC): an "active" pool supplied directly from canopy photo-assimilates supporting metabolism and a "stored" pool used when fresh C supplies are limited.
34096615	8	40	theme	~5 mg	1313:1317	arg1	"					1310:1310	"active"	1303:1310	"active" (~5 mg C g-1 )	1303:1325	Using a mixing model and observed correlations between Δ14 C of water-soluble C and α-cellulose, we estimate ~30% of C is "active" (~5 mg C g-1 ).
34096615	8	40	theme	~5 mg	1313:1317	arg1	g-1					1321:1323	~5 mg C g-1	1313:1323	~5 mg C g-1	1313:1323	Using a mixing model and observed correlations between Δ14 C of water-soluble C and α-cellulose, we estimate ~30% of C is "active" (~5 mg C g-1 ).
34096615	6	41	contain	had	951:953	arg1	roots					912:916	Freshly excised roots	896:916	Freshly excised roots from trees girdled ~3 months	896:945	Freshly excised roots from trees girdled ~3 months ago had respiration rates and NSC stocks similar to un-girdled trees but respired older C (~1.2 year).
34096615	6	41	contain	had	951:953	arg2	rates					967:971	respiration rates	955:971	respiration rates	955:971	Freshly excised roots from trees girdled ~3 months ago had respiration rates and NSC stocks similar to un-girdled trees but respired older C (~1.2 year).
34096615	6	41	contain	had	951:953	arg2	stocks					981:986	NSC stocks	977:986	NSC stocks	977:986	Freshly excised roots from trees girdled ~3 months ago had respiration rates and NSC stocks similar to un-girdled trees but respired older C (~1.2 year).
34096615	8	42	theme	water-soluble	1245:1257	arg1	C					1259:1259	water-soluble C	1245:1259	water-soluble C	1245:1259	Using a mixing model and observed correlations between Δ14 C of water-soluble C and α-cellulose, we estimate ~30% of C is "active" (~5 mg C g-1 ).
34096615	3	43	theme	stem-girdling	539:551	arg1	incubation					566:575	stem-girdling or prolonged incubation	539:575	stem-girdling or prolonged incubation of excised roots	539:592	We compared the C isotope composition of water-soluble NSC and respired CO2 for aspen roots (Populus tremula hybrids) cut off from fresh C supply after stem-girdling or prolonged incubation of excised roots.
34096615	6	44	theme	un-girdled	999:1008	arg1	trees					1010:1014	un-girdled trees	999:1014	un-girdled trees	999:1014	Freshly excised roots from trees girdled ~3 months ago had respiration rates and NSC stocks similar to un-girdled trees but respired older C (~1.2 year).
34096615	3	45	theme	isotope	405:411	arg1	composition					413:423	the C isotope composition	399:423	the C isotope composition of water-soluble NSC	399:444	We compared the C isotope composition of water-soluble NSC and respired CO2 for aspen roots (Populus tremula hybrids) cut off from fresh C supply after stem-girdling or prolonged incubation of excised roots.
34096615	3	46	theme	excised	580:586	arg1	roots					588:592	excised roots	580:592	excised roots	580:592	We compared the C isotope composition of water-soluble NSC and respired CO2 for aspen roots (Populus tremula hybrids) cut off from fresh C supply after stem-girdling or prolonged incubation of excised roots.
34096615	3	47	theme	roots	588:592	arg1	incubation					566:575	stem-girdling or prolonged incubation	539:575	stem-girdling or prolonged incubation of excised roots	539:592	We compared the C isotope composition of water-soluble NSC and respired CO2 for aspen roots (Populus tremula hybrids) cut off from fresh C supply after stem-girdling or prolonged incubation of excised roots.
34096615	3	48	theme	water-soluble	428:440	arg1	NSC					442:444	water-soluble NSC	428:444	water-soluble NSC	428:444	We compared the C isotope composition of water-soluble NSC and respired CO2 for aspen roots (Populus tremula hybrids) cut off from fresh C supply after stem-girdling or prolonged incubation of excised roots.
34096615	7	49	theme	root-external	1158:1170	arg1	sources					1172:1178	root-external sources	1158:1178	root-external sources	1158:1178	We estimate that over 3 months NSC in girdled roots must be replaced 5-7 times by reserves remobilized from root-external sources.
34096615	2	50	theme	carbohydrates	219:231	arg1	pools					195:199	two functional pools	180:199	two functional pools of non-structural carbohydrates (NSC)	180:237	Previous research in stems identified two functional pools of non-structural carbohydrates (NSC): an "active" pool supplied directly from canopy photo-assimilates supporting metabolism and a "stored" pool used when fresh C supplies are limited.
34096615	3	51	theme	C	524:524	arg1	supply					526:531	fresh C supply	518:531	fresh C supply	518:531	We compared the C isotope composition of water-soluble NSC and respired CO2 for aspen roots (Populus tremula hybrids) cut off from fresh C supply after stem-girdling or prolonged incubation of excised roots.
34096615	8	52	theme	α-cellulose	1265:1275	arg1	C					1240:1240	Δ14 C	1236:1240	Δ14 C of water-soluble C and α-cellulose	1236:1275	Using a mixing model and observed correlations between Δ14 C of water-soluble C and α-cellulose, we estimate ~30% of C is "active" (~5 mg C g-1 ).
34096615	6	53	theme	trees	923:927	arg1	~3 months					937:945	trees girdled ~3 months	923:945	trees girdled ~3 months	923:945	Freshly excised roots from trees girdled ~3 months ago had respiration rates and NSC stocks similar to un-girdled trees but respired older C (~1.2 year).
34096615	8	54	theme	Δ14	1236:1238	arg1	C					1240:1240	Δ14 C	1236:1240	Δ14 C of water-soluble C and α-cellulose	1236:1275	Using a mixing model and observed correlations between Δ14 C of water-soluble C and α-cellulose, we estimate ~30% of C is "active" (~5 mg C g-1 ).
34096615	2	55	theme	Previous	142:149	arg1	research					151:158	Previous research	142:158	Previous research in stems	142:167	Previous research in stems identified two functional pools of non-structural carbohydrates (NSC): an "active" pool supplied directly from canopy photo-assimilates supporting metabolism and a "stored" pool used when fresh C supplies are limited.
34096615	2	56	theme	non-structural	204:217	arg1	NSC					234:236	NSC	234:236	NSC	234:236	Previous research in stems identified two functional pools of non-structural carbohydrates (NSC): an "active" pool supplied directly from canopy photo-assimilates supporting metabolism and a "stored" pool used when fresh C supplies are limited.
34096615	2	56	theme	non-structural	204:217	arg1	carbohydrates					219:231	non-structural carbohydrates	204:231	non-structural carbohydrates (NSC)	204:237	Previous research in stems identified two functional pools of non-structural carbohydrates (NSC): an "active" pool supplied directly from canopy photo-assimilates supporting metabolism and a "stored" pool used when fresh C supplies are limited.
34096615	3	57	theme	C	403:403	arg1	composition					413:423	the C isotope composition	399:423	the C isotope composition of water-soluble NSC	399:444	We compared the C isotope composition of water-soluble NSC and respired CO2 for aspen roots (Populus tremula hybrids) cut off from fresh C supply after stem-girdling or prolonged incubation of excised roots.
34096615	8	58	theme	observed	1206:1213	arg1	correlations					1215:1226	observed correlations	1206:1226	observed correlations between Δ14 C of water-soluble C and α-cellulose	1206:1275	Using a mixing model and observed correlations between Δ14 C of water-soluble C and α-cellulose, we estimate ~30% of C is "active" (~5 mg C g-1 ).
34096615	4	59	theme	water-soluble	685:697	arg1	NSC					699:701	water-soluble NSC	685:701	water-soluble NSC	685:701	We used bomb radiocarbon to estimate the time elapsed since C fixation for respired CO2 , water-soluble NSC and structural α-cellulose.
34096615	0	60	theme	carbon	49:54	arg1	size					4:7	The size	0:7	The size	0:7	The size and the age of the metabolically active carbon in tree roots.
34096615	0	60	theme	carbon	49:54	arg1	age					17:19	the age	13:19	the age of the metabolically active carbon in tree roots	13:68	The size and the age of the metabolically active carbon in tree roots.
34096615	6	61	theme	girdled	929:935	arg1	~3 months					937:945	trees girdled ~3 months	923:945	trees girdled ~3 months	923:945	Freshly excised roots from trees girdled ~3 months ago had respiration rates and NSC stocks similar to un-girdled trees but respired older C (~1.2 year).
34096615	8	62	theme	C	1298:1298	arg1	%					1293:1293	~30%	1290:1293	~30% of C	1290:1298	Using a mixing model and observed correlations between Δ14 C of water-soluble C and α-cellulose, we estimate ~30% of C is "active" (~5 mg C g-1 ).
34096615	8	62	theme	C	1298:1298	arg1	C					1298:1298	C	1298:1298	C	1298:1298	Using a mixing model and observed correlations between Δ14 C of water-soluble C and α-cellulose, we estimate ~30% of C is "active" (~5 mg C g-1 ).
34096615	5	63	from	diameter	778:785	arg1	<2.9 mm					767:773	<2.9 mm	767:773	<2.9 mm	767:773	While freshly excised roots (mostly <2.9 mm in diameter) respired CO2 fixed <1 year previously, the age increased to 1.6-2.9 year within a week after root excision.
34096615	3	64	theme	NSC	442:444	arg1	composition					413:423	the C isotope composition	399:423	the C isotope composition of water-soluble NSC	399:444	We compared the C isotope composition of water-soluble NSC and respired CO2 for aspen roots (Populus tremula hybrids) cut off from fresh C supply after stem-girdling or prolonged incubation of excised roots.
34096615	0	65	theme	active	42:47	arg1	carbon					49:54	the metabolically active carbon	24:54	the metabolically active carbon	24:54	The size and the age of the metabolically active carbon in tree roots.
34096615	3	66	theme	fresh	518:522	arg1	supply					526:531	fresh C supply	518:531	fresh C supply	518:531	We compared the C isotope composition of water-soluble NSC and respired CO2 for aspen roots (Populus tremula hybrids) cut off from fresh C supply after stem-girdling or prolonged incubation of excised roots.
34096615	3	67	theme	tremula	488:494	arg1	hybrids					496:502	Populus tremula hybrids	480:502	Populus tremula hybrids	480:502	We compared the C isotope composition of water-soluble NSC and respired CO2 for aspen roots (Populus tremula hybrids) cut off from fresh C supply after stem-girdling or prolonged incubation of excised roots.
34096615	3	67	theme	tremula	488:494	arg1	roots					473:477	aspen roots	467:477	aspen roots (Populus tremula hybrids) cut off from fresh C supply after stem-girdling or prolonged incubation of excised roots	467:592	We compared the C isotope composition of water-soluble NSC and respired CO2 for aspen roots (Populus tremula hybrids) cut off from fresh C supply after stem-girdling or prolonged incubation of excised roots.
34096615	6	68	theme	excised	904:910	arg1	roots					912:916	Freshly excised roots	896:916	Freshly excised roots from trees girdled ~3 months	896:945	Freshly excised roots from trees girdled ~3 months ago had respiration rates and NSC stocks similar to un-girdled trees but respired older C (~1.2 year).
32530045	8	0	theme	greater	1326:1332	arg1	body					1334:1337	their greater body	1320:1337	their greater body	1320:1337	Furthermore, LEGCHIT wines were appreciated by panelists for their greater body and reduced astringency perception.
32530045	4	1	theme	wines	757:761	arg1	composition					700:710	volatile composition	691:710	volatile composition	691:710	The clarification efficiency, total polyphenols, and total proteins were determined in the grape must before and after flotation, as well as the volatile composition and sensory characteristics of the resulting wines.
32530045	4	1	theme	wines	757:761	arg1	characteristics					724:738	sensory characteristics	716:738	sensory characteristics	716:738	The clarification efficiency, total polyphenols, and total proteins were determined in the grape must before and after flotation, as well as the volatile composition and sensory characteristics of the resulting wines.
32530045	4	1	theme	wines	757:761	arg1	flotation					665:673	flotation	665:673	flotation	665:673	The clarification efficiency, total polyphenols, and total proteins were determined in the grape must before and after flotation, as well as the volatile composition and sensory characteristics of the resulting wines.
32530045	0	2	dep	vinifera	107:114	arg1	L.					116:117	Vitis vinifera L.	101:117	Vitis vinifera L.	101:117	Effect of flotation and vegetal fining agents on the aromatic characteristics of Malvasia del Lazio (Vitis vinifera L.) wine.
32530045	7	3	theme	volatile	1082:1089	arg1	VOCs					1110:1113	VOCs	1110:1113	VOCs	1110:1113	The vegetal protein treatments caused a decrease in the concentration of volatile organic compounds (VOCs) with respect to gelatin, which resulted in a reduction of aroma intensity, particularly for fruity and floral notes, but also for green notes.
32530045	7	3	theme	volatile	1082:1089	arg1	compounds					1099:1107	volatile organic compounds	1082:1107	volatile organic compounds (VOCs) with respect to gelatin, which resulted in a reduction of aroma intensity, particularly for fruity and floral notes, but also for green notes	1082:1256	The vegetal protein treatments caused a decrease in the concentration of volatile organic compounds (VOCs) with respect to gelatin, which resulted in a reduction of aroma intensity, particularly for fruity and floral notes, but also for green notes.
32530045	8	4	theme	reduced	1343:1349	arg1	perception					1363:1372	reduced astringency perception	1343:1372	reduced astringency perception	1343:1372	Furthermore, LEGCHIT wines were appreciated by panelists for their greater body and reduced astringency perception.
32530045	4	5	theme	resulting	747:755	arg1	wines					757:761	the resulting wines	743:761	the resulting wines	743:761	The clarification efficiency, total polyphenols, and total proteins were determined in the grape must before and after flotation, as well as the volatile composition and sensory characteristics of the resulting wines.
32530045	6	6	theme	total	986:990	arg1	content					1000:1006	the highest total protein content	974:1006	LEGYEAST but also the highest total protein content	956:1006	The GEL trial was the slowest, showing grape must turbidity values similar to LEGYEAST but also the highest total protein content.
32530045	0	7	theme	del	90:92	arg1	Lazio					94:98	Malvasia del Lazio	81:98	Malvasia del Lazio (Vitis vinifera L.) wine	81:123	Effect of flotation and vegetal fining agents on the aromatic characteristics of Malvasia del Lazio (Vitis vinifera L.) wine.
32530045	0	7	theme	del	90:92	arg1	vinifera					107:114	Vitis vinifera L.	101:117	Vitis vinifera L.	101:117	Effect of flotation and vegetal fining agents on the aromatic characteristics of Malvasia del Lazio (Vitis vinifera L.) wine.
32530045	0	8	from	Effect	0:5	arg1	characteristics					62:76	the aromatic characteristics	49:76	the aromatic characteristics of Malvasia del Lazio (Vitis vinifera L.) wine	49:123	Effect of flotation and vegetal fining agents on the aromatic characteristics of Malvasia del Lazio (Vitis vinifera L.) wine.
32530045	3	9	theme	legume	458:463	arg1	protein					465:471	legume protein	458:471	legume protein	458:471	Malvasia del Lazio juice (Vitis vinifera L.) was treated before flotation with animal gelatin (GEL), legume protein plus chitin (LEGCHIT), and legume protein plus yeast extract (LEGYEAST).
32530045	3	9	theme	legume	458:463	arg1	LEGCHIT					486:492	LEGCHIT	486:492	LEGCHIT	486:492	Malvasia del Lazio juice (Vitis vinifera L.) was treated before flotation with animal gelatin (GEL), legume protein plus chitin (LEGCHIT), and legume protein plus yeast extract (LEGYEAST).
32530045	10	10	theme	higher	1618:1623	arg1	loss					1625:1628	a higher loss	1616:1628	a higher loss (38% and 27% respectively for LEGYEAST and LEGCHIT) of VOCs	1616:1688	Furthermore, the wines obtained showed high perceived global quality, even though a higher loss (38% and 27% respectively for LEGYEAST and LEGCHIT) of VOCs occurred when compared with gelatin.
32530045	0	11	theme	Malvasia	81:88	arg1	Lazio					94:98	Malvasia del Lazio	81:98	Malvasia del Lazio (Vitis vinifera L.) wine	81:123	Effect of flotation and vegetal fining agents on the aromatic characteristics of Malvasia del Lazio (Vitis vinifera L.) wine.
32530045	0	11	theme	Malvasia	81:88	arg1	vinifera					107:114	Vitis vinifera L.	101:117	Vitis vinifera L.	101:117	Effect of flotation and vegetal fining agents on the aromatic characteristics of Malvasia del Lazio (Vitis vinifera L.) wine.
32530045	10	12	theme	perceived	1578:1586	arg1	quality					1595:1601	high perceived global quality	1573:1601	high perceived global quality	1573:1601	Furthermore, the wines obtained showed high perceived global quality, even though a higher loss (38% and 27% respectively for LEGYEAST and LEGCHIT) of VOCs occurred when compared with gelatin.
32530045	9	13	theme	clarification	1493:1505	arg1	efficiency					1507:1516	clarification efficiency	1493:1516	clarification efficiency	1493:1516	CONCLUSION The use of legume protein combined with chitin as a fining agent for flotation is advantageous in terms of clarification efficiency for grape must.
32530045	1	14	theme	BACKGROUND	126:135	arg1	process					152:158	a process	150:158	a process to reduce the must turbidity after grape pressing	150:208	BACKGROUND Flotation is a process to reduce the must turbidity after grape pressing.
32530045	1	14	theme	BACKGROUND	126:135	arg1	Flotation					137:145	BACKGROUND Flotation	126:145	BACKGROUND Flotation	126:145	BACKGROUND Flotation is a process to reduce the must turbidity after grape pressing.
32530045	6	15	theme	protein	992:998	arg1	content					1000:1006	the highest total protein content	974:1006	LEGYEAST but also the highest total protein content	956:1006	The GEL trial was the slowest, showing grape must turbidity values similar to LEGYEAST but also the highest total protein content.
32530045	9	16	theme	efficiency	1507:1516	arg1	terms					1484:1488	terms	1484:1488	terms of clarification efficiency	1484:1516	CONCLUSION The use of legume protein combined with chitin as a fining agent for flotation is advantageous in terms of clarification efficiency for grape must.
32530045	6	17	theme	similar	945:951	arg1	values					938:943	turbidity values	928:943	turbidity values similar to LEGYEAST but also the highest total protein content	928:1006	The GEL trial was the slowest, showing grape must turbidity values similar to LEGYEAST but also the highest total protein content.
32530045	6	18	dep	grape	917:921	arg1	must					923:926	must	923:926	must	923:926	The GEL trial was the slowest, showing grape must turbidity values similar to LEGYEAST but also the highest total protein content.
32530045	6	19	theme	turbidity	928:936	arg1	values					938:943	turbidity values	928:943	turbidity values similar to LEGYEAST but also the highest total protein content	928:1006	The GEL trial was the slowest, showing grape must turbidity values similar to LEGYEAST but also the highest total protein content.
32530045	4	20	theme	clarification	550:562	arg1	efficiency					564:573	The clarification efficiency	546:573	The clarification efficiency	546:573	The clarification efficiency, total polyphenols, and total proteins were determined in the grape must before and after flotation, as well as the volatile composition and sensory characteristics of the resulting wines.
32530045	0	21	theme	Lazio	94:98	arg1	wine					120:123	Malvasia del Lazio (Vitis vinifera L.) wine	81:123	Malvasia del Lazio (Vitis vinifera L.) wine	81:123	Effect of flotation and vegetal fining agents on the aromatic characteristics of Malvasia del Lazio (Vitis vinifera L.) wine.
32530045	3	22	theme	Malvasia	357:364	arg1	juice					376:380	Malvasia del Lazio juice	357:380	Malvasia del Lazio juice (Vitis vinifera L.)	357:400	Malvasia del Lazio juice (Vitis vinifera L.) was treated before flotation with animal gelatin (GEL), legume protein plus chitin (LEGCHIT), and legume protein plus yeast extract (LEGYEAST).
32530045	3	22	theme	Malvasia	357:364	arg1	vinifera					389:396	Vitis vinifera L.	383:399	Vitis vinifera L.	383:399	Malvasia del Lazio juice (Vitis vinifera L.) was treated before flotation with animal gelatin (GEL), legume protein plus chitin (LEGCHIT), and legume protein plus yeast extract (LEGYEAST).
32530045	9	23	from	advantageous	1468:1479	arg1	terms					1484:1488	terms	1484:1488	terms of clarification efficiency	1484:1516	CONCLUSION The use of legume protein combined with chitin as a fining agent for flotation is advantageous in terms of clarification efficiency for grape must.
32530045	2	24	theme	agents	229:234	arg1	use					215:217	The use	211:217	The use of fining agents to reduce the polyphenol content	211:267	The use of fining agents to reduce the polyphenol content is essential for white wine, but their impact on volatile compounds must be considered.
32530045	2	24	theme	agents	229:234	arg1	essential					272:280	essential	272:280	essential	272:280	The use of fining agents to reduce the polyphenol content is essential for white wine, but their impact on volatile compounds must be considered.
32530045	9	25	dep	CONCLUSION	1375:1384	arg1	use					1390:1392	The use	1386:1392	The use of legume protein combined with chitin as a fining agent for flotation	1386:1463	CONCLUSION The use of legume protein combined with chitin as a fining agent for flotation is advantageous in terms of clarification efficiency for grape must.
32530045	9	25	dep	CONCLUSION	1375:1384	arg1	advantageous					1468:1479	advantageous	1468:1479	advantageous	1468:1479	CONCLUSION The use of legume protein combined with chitin as a fining agent for flotation is advantageous in terms of clarification efficiency for grape must.
32530045	3	26	theme	Vitis	383:387	arg1	juice					376:380	Malvasia del Lazio juice	357:380	Malvasia del Lazio juice (Vitis vinifera L.)	357:400	Malvasia del Lazio juice (Vitis vinifera L.) was treated before flotation with animal gelatin (GEL), legume protein plus chitin (LEGCHIT), and legume protein plus yeast extract (LEGYEAST).
32530045	3	26	theme	Vitis	383:387	arg1	vinifera					389:396	Vitis vinifera L.	383:399	Vitis vinifera L.	383:399	Malvasia del Lazio juice (Vitis vinifera L.) was treated before flotation with animal gelatin (GEL), legume protein plus chitin (LEGCHIT), and legume protein plus yeast extract (LEGYEAST).
32530045	10	27	theme	VOCs	1685:1688	arg1	loss					1625:1628	a higher loss	1616:1628	a higher loss (38% and 27% respectively for LEGYEAST and LEGCHIT) of VOCs	1616:1688	Furthermore, the wines obtained showed high perceived global quality, even though a higher loss (38% and 27% respectively for LEGYEAST and LEGCHIT) of VOCs occurred when compared with gelatin.
32530045	10	28	theme	global	1588:1593	arg1	quality					1595:1601	high perceived global quality	1573:1601	high perceived global quality	1573:1601	Furthermore, the wines obtained showed high perceived global quality, even though a higher loss (38% and 27% respectively for LEGYEAST and LEGCHIT) of VOCs occurred when compared with gelatin.
32530045	7	29	theme	organic	1091:1097	arg1	VOCs					1110:1113	VOCs	1110:1113	VOCs	1110:1113	The vegetal protein treatments caused a decrease in the concentration of volatile organic compounds (VOCs) with respect to gelatin, which resulted in a reduction of aroma intensity, particularly for fruity and floral notes, but also for green notes.
32530045	7	29	theme	organic	1091:1097	arg1	compounds					1099:1107	volatile organic compounds	1082:1107	volatile organic compounds (VOCs) with respect to gelatin, which resulted in a reduction of aroma intensity, particularly for fruity and floral notes, but also for green notes	1082:1256	The vegetal protein treatments caused a decrease in the concentration of volatile organic compounds (VOCs) with respect to gelatin, which resulted in a reduction of aroma intensity, particularly for fruity and floral notes, but also for green notes.
32530045	9	30	theme	protein	1404:1410	arg1	use					1390:1392	The use	1386:1392	The use of legume protein combined with chitin as a fining agent for flotation	1386:1463	CONCLUSION The use of legume protein combined with chitin as a fining agent for flotation is advantageous in terms of clarification efficiency for grape must.
32530045	9	30	theme	protein	1404:1410	arg1	advantageous					1468:1479	advantageous	1468:1479	advantageous	1468:1479	CONCLUSION The use of legume protein combined with chitin as a fining agent for flotation is advantageous in terms of clarification efficiency for grape must.
32530045	8	31	theme	astringency	1351:1361	arg1	perception					1363:1372	reduced astringency perception	1343:1372	reduced astringency perception	1343:1372	Furthermore, LEGCHIT wines were appreciated by panelists for their greater body and reduced astringency perception.
32530045	3	32	theme	Lazio	370:374	arg1	juice					376:380	Malvasia del Lazio juice	357:380	Malvasia del Lazio juice (Vitis vinifera L.)	357:400	Malvasia del Lazio juice (Vitis vinifera L.) was treated before flotation with animal gelatin (GEL), legume protein plus chitin (LEGCHIT), and legume protein plus yeast extract (LEGYEAST).
32530045	3	32	theme	Lazio	370:374	arg1	vinifera					389:396	Vitis vinifera L.	383:399	Vitis vinifera L.	383:399	Malvasia del Lazio juice (Vitis vinifera L.) was treated before flotation with animal gelatin (GEL), legume protein plus chitin (LEGCHIT), and legume protein plus yeast extract (LEGYEAST).
32530045	7	33	theme	vegetal	1013:1019	arg1	treatments					1029:1038	The vegetal protein treatments	1009:1038	The vegetal protein treatments	1009:1038	The vegetal protein treatments caused a decrease in the concentration of volatile organic compounds (VOCs) with respect to gelatin, which resulted in a reduction of aroma intensity, particularly for fruity and floral notes, but also for green notes.
32530045	7	34	theme	green	1246:1250	arg1	notes					1252:1256	green notes	1246:1256	green notes	1246:1256	The vegetal protein treatments caused a decrease in the concentration of volatile organic compounds (VOCs) with respect to gelatin, which resulted in a reduction of aroma intensity, particularly for fruity and floral notes, but also for green notes.
32530045	2	35	theme	volatile	318:325	arg1	compounds					327:335	volatile compounds	318:335	volatile compounds	318:335	The use of fining agents to reduce the polyphenol content is essential for white wine, but their impact on volatile compounds must be considered.
32530045	7	36	theme	protein	1021:1027	arg1	treatments					1029:1038	The vegetal protein treatments	1009:1038	The vegetal protein treatments	1009:1038	The vegetal protein treatments caused a decrease in the concentration of volatile organic compounds (VOCs) with respect to gelatin, which resulted in a reduction of aroma intensity, particularly for fruity and floral notes, but also for green notes.
32530045	11	37	theme	Chemical	1745:1752	arg1	Industry					1754:1761	Chemical Industry	1745:1761	Chemical Industry	1745:1761	© 2020 Society of Chemical Industry.
32530045	2	38	from	impact	308:313	arg1	compounds					327:335	volatile compounds	318:335	volatile compounds	318:335	The use of fining agents to reduce the polyphenol content is essential for white wine, but their impact on volatile compounds must be considered.
32530045	0	39	theme	flotation	10:18	arg1	Effect					0:5	Effect	0:5	Effect of flotation and vegetal fining agents on the aromatic characteristics of Malvasia del Lazio (Vitis vinifera L.) wine.	0:124	Effect of flotation and vegetal fining agents on the aromatic characteristics of Malvasia del Lazio (Vitis vinifera L.) wine.
32530045	0	40	theme	Vitis	101:105	arg1	Lazio					94:98	Malvasia del Lazio	81:98	Malvasia del Lazio (Vitis vinifera L.) wine	81:123	Effect of flotation and vegetal fining agents on the aromatic characteristics of Malvasia del Lazio (Vitis vinifera L.) wine.
32530045	0	40	theme	Vitis	101:105	arg1	vinifera					107:114	Vitis vinifera L.	101:117	Vitis vinifera L.	101:117	Effect of flotation and vegetal fining agents on the aromatic characteristics of Malvasia del Lazio (Vitis vinifera L.) wine.
32530045	7	41	from	decrease	1049:1056	arg1	concentration					1065:1077	the concentration	1061:1077	the concentration of volatile organic compounds (VOCs) with respect to gelatin, which resulted in a reduction of aroma intensity, particularly for fruity and floral notes, but also for green notes	1061:1256	The vegetal protein treatments caused a decrease in the concentration of volatile organic compounds (VOCs) with respect to gelatin, which resulted in a reduction of aroma intensity, particularly for fruity and floral notes, but also for green notes.
32530045	2	42	theme	polyphenol	250:259	arg1	content					261:267	the polyphenol content	246:267	the polyphenol content	246:267	The use of fining agents to reduce the polyphenol content is essential for white wine, but their impact on volatile compounds must be considered.
32530045	4	43	dep	composition	700:710	arg1	the					687:689	the	687:689	the	687:689	The clarification efficiency, total polyphenols, and total proteins were determined in the grape must before and after flotation, as well as the volatile composition and sensory characteristics of the resulting wines.
32530045	0	44	theme	fining	32:37	arg1	agents					39:44	vegetal fining agents	24:44	vegetal fining agents	24:44	Effect of flotation and vegetal fining agents on the aromatic characteristics of Malvasia del Lazio (Vitis vinifera L.) wine.
32530045	4	45	dep	grape	637:641	arg1	must					643:646	must	643:646	must	643:646	The clarification efficiency, total polyphenols, and total proteins were determined in the grape must before and after flotation, as well as the volatile composition and sensory characteristics of the resulting wines.
32530045	8	46	theme	LEGCHIT	1272:1278	arg1	wines					1280:1284	LEGCHIT wines	1272:1284	LEGCHIT wines	1272:1284	Furthermore, LEGCHIT wines were appreciated by panelists for their greater body and reduced astringency perception.
32530045	4	47	theme	volatile	691:698	arg1	composition					700:710	volatile composition	691:710	volatile composition	691:710	The clarification efficiency, total polyphenols, and total proteins were determined in the grape must before and after flotation, as well as the volatile composition and sensory characteristics of the resulting wines.
32530045	0	48	theme	vegetal	24:30	arg1	agents					39:44	vegetal fining agents	24:44	vegetal fining agents	24:44	Effect of flotation and vegetal fining agents on the aromatic characteristics of Malvasia del Lazio (Vitis vinifera L.) wine.
32530045	3	49	theme	del	366:368	arg1	juice					376:380	Malvasia del Lazio juice	357:380	Malvasia del Lazio juice (Vitis vinifera L.)	357:400	Malvasia del Lazio juice (Vitis vinifera L.) was treated before flotation with animal gelatin (GEL), legume protein plus chitin (LEGCHIT), and legume protein plus yeast extract (LEGYEAST).
32530045	3	49	theme	del	366:368	arg1	vinifera					389:396	Vitis vinifera L.	383:399	Vitis vinifera L.	383:399	Malvasia del Lazio juice (Vitis vinifera L.) was treated before flotation with animal gelatin (GEL), legume protein plus chitin (LEGCHIT), and legume protein plus yeast extract (LEGYEAST).
32530045	7	50	theme	aroma	1174:1178	arg1	intensity					1180:1188	aroma intensity	1174:1188	aroma intensity	1174:1188	The vegetal protein treatments caused a decrease in the concentration of volatile organic compounds (VOCs) with respect to gelatin, which resulted in a reduction of aroma intensity, particularly for fruity and floral notes, but also for green notes.
32530045	3	51	theme	legume	500:505	arg1	LEGYEAST					535:542	LEGYEAST	535:542	LEGYEAST	535:542	Malvasia del Lazio juice (Vitis vinifera L.) was treated before flotation with animal gelatin (GEL), legume protein plus chitin (LEGCHIT), and legume protein plus yeast extract (LEGYEAST).
32530045	3	51	theme	legume	500:505	arg1	protein					507:513	legume protein	500:513	legume protein	500:513	Malvasia del Lazio juice (Vitis vinifera L.) was treated before flotation with animal gelatin (GEL), legume protein plus chitin (LEGCHIT), and legume protein plus yeast extract (LEGYEAST).
32530045	7	52	theme	floral	1219:1224	arg1	notes					1226:1230	floral notes	1219:1230	floral notes	1219:1230	The vegetal protein treatments caused a decrease in the concentration of volatile organic compounds (VOCs) with respect to gelatin, which resulted in a reduction of aroma intensity, particularly for fruity and floral notes, but also for green notes.
32530045	3	53	theme	animal	436:441	arg1	gelatin					443:449	animal gelatin	436:449	animal gelatin (GEL)	436:455	Malvasia del Lazio juice (Vitis vinifera L.) was treated before flotation with animal gelatin (GEL), legume protein plus chitin (LEGCHIT), and legume protein plus yeast extract (LEGYEAST).
32530045	3	53	theme	animal	436:441	arg1	GEL					452:454	GEL	452:454	GEL	452:454	Malvasia del Lazio juice (Vitis vinifera L.) was treated before flotation with animal gelatin (GEL), legume protein plus chitin (LEGCHIT), and legume protein plus yeast extract (LEGYEAST).
32530045	10	54	dep	loss	1625:1628	arg1	%					1633:1633	38%	1631:1633	38%	1631:1633	Furthermore, the wines obtained showed high perceived global quality, even though a higher loss (38% and 27% respectively for LEGYEAST and LEGCHIT) of VOCs occurred when compared with gelatin.
32530045	10	54	dep	loss	1625:1628	arg1	%					1641:1641	27%	1639:1641	27%	1639:1641	Furthermore, the wines obtained showed high perceived global quality, even though a higher loss (38% and 27% respectively for LEGYEAST and LEGCHIT) of VOCs occurred when compared with gelatin.
32530045	7	55	theme	compounds	1099:1107	arg1	concentration					1065:1077	the concentration	1061:1077	the concentration of volatile organic compounds (VOCs) with respect to gelatin, which resulted in a reduction of aroma intensity, particularly for fruity and floral notes, but also for green notes	1061:1256	The vegetal protein treatments caused a decrease in the concentration of volatile organic compounds (VOCs) with respect to gelatin, which resulted in a reduction of aroma intensity, particularly for fruity and floral notes, but also for green notes.
32530045	9	56	theme	fining	1438:1443	arg1	agent					1445:1449	a fining agent	1436:1449	a fining agent for flotation	1436:1463	CONCLUSION The use of legume protein combined with chitin as a fining agent for flotation is advantageous in terms of clarification efficiency for grape must.
32530045	6	57	theme	highest	978:984	arg1	content					1000:1006	the highest total protein content	974:1006	LEGYEAST but also the highest total protein content	956:1006	The GEL trial was the slowest, showing grape must turbidity values similar to LEGYEAST but also the highest total protein content.
32530045	3	58	dep	vinifera	389:396	arg1	L.					398:399	L.	398:399	L.	398:399	Malvasia del Lazio juice (Vitis vinifera L.) was treated before flotation with animal gelatin (GEL), legume protein plus chitin (LEGCHIT), and legume protein plus yeast extract (LEGYEAST).
32530045	0	59	theme	agents	39:44	arg1	Effect					0:5	Effect	0:5	Effect of flotation and vegetal fining agents on the aromatic characteristics of Malvasia del Lazio (Vitis vinifera L.) wine.	0:124	Effect of flotation and vegetal fining agents on the aromatic characteristics of Malvasia del Lazio (Vitis vinifera L.) wine.
32530045	0	60	theme	wine	120:123	arg1	characteristics					62:76	the aromatic characteristics	49:76	the aromatic characteristics of Malvasia del Lazio (Vitis vinifera L.) wine	49:123	Effect of flotation and vegetal fining agents on the aromatic characteristics of Malvasia del Lazio (Vitis vinifera L.) wine.
32530045	4	61	theme	sensory	716:722	arg1	characteristics					724:738	sensory characteristics	716:738	sensory characteristics	716:738	The clarification efficiency, total polyphenols, and total proteins were determined in the grape must before and after flotation, as well as the volatile composition and sensory characteristics of the resulting wines.
32530045	4	62	theme	total	599:603	arg1	proteins					605:612	total proteins	599:612	total proteins	599:612	The clarification efficiency, total polyphenols, and total proteins were determined in the grape must before and after flotation, as well as the volatile composition and sensory characteristics of the resulting wines.
32530045	9	63	from	terms	1484:1488	arg1	use					1390:1392	The use	1386:1392	The use of legume protein combined with chitin as a fining agent for flotation	1386:1463	CONCLUSION The use of legume protein combined with chitin as a fining agent for flotation is advantageous in terms of clarification efficiency for grape must.
32530045	9	63	from	terms	1484:1488	arg1	advantageous					1468:1479	advantageous	1468:1479	advantageous	1468:1479	CONCLUSION The use of legume protein combined with chitin as a fining agent for flotation is advantageous in terms of clarification efficiency for grape must.
32530045	3	64	theme	yeast	520:524	arg1	extract					526:532	yeast extract	520:532	yeast extract	520:532	Malvasia del Lazio juice (Vitis vinifera L.) was treated before flotation with animal gelatin (GEL), legume protein plus chitin (LEGCHIT), and legume protein plus yeast extract (LEGYEAST).
32530045	2	65	theme	fining	222:227	arg1	agents					229:234	fining agents	222:234	fining agents	222:234	The use of fining agents to reduce the polyphenol content is essential for white wine, but their impact on volatile compounds must be considered.
32530045	5	66	dep	RESULTS	764:770	arg1	trial					784:788	The LEGCHIT trial	772:788	The LEGCHIT trial	772:788	RESULTS The LEGCHIT trial was the most efficient, it being the fastest and achieving the lowest turbidity values.
32530045	5	66	dep	RESULTS	764:770	arg1	achieving					839:847	achieving	839:847	achieving the lowest turbidity values	839:875	RESULTS The LEGCHIT trial was the most efficient, it being the fastest and achieving the lowest turbidity values.
32530045	5	66	dep	RESULTS	764:770	arg1	fastest					827:833	fastest	827:833	fastest	827:833	RESULTS The LEGCHIT trial was the most efficient, it being the fastest and achieving the lowest turbidity values.
32530045	5	66	dep	RESULTS	764:770	arg1	efficient					803:811	efficient	803:811	efficient	803:811	RESULTS The LEGCHIT trial was the most efficient, it being the fastest and achieving the lowest turbidity values.
32530045	2	67	theme	white	286:290	arg1	wine					292:295	white wine	286:295	white wine	286:295	The use of fining agents to reduce the polyphenol content is essential for white wine, but their impact on volatile compounds must be considered.
32530045	5	68	theme	lowest	853:858	arg1	values					870:875	the lowest turbidity values	849:875	the lowest turbidity values	849:875	RESULTS The LEGCHIT trial was the most efficient, it being the fastest and achieving the lowest turbidity values.
32530045	9	69	theme	legume	1397:1402	arg1	protein					1404:1410	legume protein	1397:1410	legume protein	1397:1410	CONCLUSION The use of legume protein combined with chitin as a fining agent for flotation is advantageous in terms of clarification efficiency for grape must.
32530045	5	70	dep	efficient	803:811	arg1	trial					784:788	The LEGCHIT trial	772:788	The LEGCHIT trial	772:788	RESULTS The LEGCHIT trial was the most efficient, it being the fastest and achieving the lowest turbidity values.
32530045	5	70	dep	efficient	803:811	arg1	achieving					839:847	achieving	839:847	achieving the lowest turbidity values	839:875	RESULTS The LEGCHIT trial was the most efficient, it being the fastest and achieving the lowest turbidity values.
32530045	5	70	dep	efficient	803:811	arg1	it					814:815	it	814:815	it	814:815	RESULTS The LEGCHIT trial was the most efficient, it being the fastest and achieving the lowest turbidity values.
32530045	5	70	dep	efficient	803:811	arg1	fastest					827:833	fastest	827:833	fastest	827:833	RESULTS The LEGCHIT trial was the most efficient, it being the fastest and achieving the lowest turbidity values.
32530045	5	70	dep	efficient	803:811	arg1	efficient					803:811	efficient	803:811	efficient	803:811	RESULTS The LEGCHIT trial was the most efficient, it being the fastest and achieving the lowest turbidity values.
32530045	5	71	theme	LEGCHIT	776:782	arg1	trial					784:788	The LEGCHIT trial	772:788	The LEGCHIT trial	772:788	RESULTS The LEGCHIT trial was the most efficient, it being the fastest and achieving the lowest turbidity values.
32530045	5	71	theme	LEGCHIT	776:782	arg1	achieving					839:847	achieving	839:847	achieving the lowest turbidity values	839:875	RESULTS The LEGCHIT trial was the most efficient, it being the fastest and achieving the lowest turbidity values.
32530045	5	71	theme	LEGCHIT	776:782	arg1	it					814:815	it	814:815	it	814:815	RESULTS The LEGCHIT trial was the most efficient, it being the fastest and achieving the lowest turbidity values.
32530045	5	71	theme	LEGCHIT	776:782	arg1	fastest					827:833	fastest	827:833	fastest	827:833	RESULTS The LEGCHIT trial was the most efficient, it being the fastest and achieving the lowest turbidity values.
32530045	5	71	theme	LEGCHIT	776:782	arg1	efficient					803:811	efficient	803:811	efficient	803:811	RESULTS The LEGCHIT trial was the most efficient, it being the fastest and achieving the lowest turbidity values.
32530045	1	72	theme	grape	195:199	arg1	pressing					201:208	grape pressing	195:208	grape pressing	195:208	BACKGROUND Flotation is a process to reduce the must turbidity after grape pressing.
32530045	0	73	theme	aromatic	53:60	arg1	characteristics					62:76	the aromatic characteristics	49:76	the aromatic characteristics of Malvasia del Lazio (Vitis vinifera L.) wine	49:123	Effect of flotation and vegetal fining agents on the aromatic characteristics of Malvasia del Lazio (Vitis vinifera L.) wine.
32530045	10	74	theme	high	1573:1576	arg1	quality					1595:1601	high perceived global quality	1573:1601	high perceived global quality	1573:1601	Furthermore, the wines obtained showed high perceived global quality, even though a higher loss (38% and 27% respectively for LEGYEAST and LEGCHIT) of VOCs occurred when compared with gelatin.
32530045	7	75	theme	intensity	1180:1188	arg1	reduction					1161:1169	a reduction	1159:1169	a reduction of aroma intensity	1159:1188	The vegetal protein treatments caused a decrease in the concentration of volatile organic compounds (VOCs) with respect to gelatin, which resulted in a reduction of aroma intensity, particularly for fruity and floral notes, but also for green notes.
32530045	5	76	theme	turbidity	860:868	arg1	values					870:875	the lowest turbidity values	849:875	the lowest turbidity values	849:875	RESULTS The LEGCHIT trial was the most efficient, it being the fastest and achieving the lowest turbidity values.
32530045	4	77	theme	total	576:580	arg1	polyphenols					582:592	total polyphenols	576:592	total polyphenols	576:592	The clarification efficiency, total polyphenols, and total proteins were determined in the grape must before and after flotation, as well as the volatile composition and sensory characteristics of the resulting wines.
32530045	6	78	theme	GEL	882:884	arg1	trial					886:890	The GEL trial	878:890	The GEL trial	878:890	The GEL trial was the slowest, showing grape must turbidity values similar to LEGYEAST but also the highest total protein content.
32530045	6	78	theme	GEL	882:884	arg1	slowest					900:906	slowest	900:906	slowest	900:906	The GEL trial was the slowest, showing grape must turbidity values similar to LEGYEAST but also the highest total protein content.
34560940	8	0	theme	low-cost	1109:1116	arg1	formulation					1123:1133	a low-cost drug formulation	1107:1133	a low-cost drug formulation for colorectal cancer treatments	1107:1166	This easily synthesized MC/5-FU indicated the potential in application as a low-cost drug formulation for colorectal cancer treatments.
34560940	4	1	theme	Transmission	510:521	arg1	microscopy					545:554	Transmission and scanning electron microscopy	510:554	microscopy	545:554	Transmission and scanning electron microscopy study demonstrated that Fe3O4 nanofillers covered the cellulose matrix.
34560940	8	2	theme	cancer	1150:1155	arg1	treatments					1157:1166	colorectal cancer treatments	1139:1166	colorectal cancer treatments	1139:1166	This easily synthesized MC/5-FU indicated the potential in application as a low-cost drug formulation for colorectal cancer treatments.
34560940	5	3	from	MC/5-FU	650:656	arg1	release					637:643	The drug release	628:643	The drug release from MC/5-FU	628:656	The drug release from MC/5-FU was evaluated under various pH and temperature conditions, showing the maximum release at pH 7.4 and 44.2 °C.
34560940	2	4	theme	colorectal	392:401	arg1	treatments					410:419	potential colorectal cancer treatments	382:419	potential colorectal cancer treatments	382:419	Herein, Fe3O4-nanoparticles were supported on rice straw cellulose for 5-fluorouracil carrier abbreviated as MC/5-FU for potential colorectal cancer treatments.
34560940	3	5	theme	multifunctional	453:467	arg1	properties					469:478	the multifunctional properties	449:478	the multifunctional properties of MC/5-FU bionanocomposites	449:507	Several analyses indicated the multifunctional properties of MC/5-FU bionanocomposites.
34560940	4	6	theme	cellulose	610:618	arg1	matrix					620:625	the cellulose matrix	606:625	the cellulose matrix	606:625	Transmission and scanning electron microscopy study demonstrated that Fe3O4 nanofillers covered the cellulose matrix.
34560940	6	7	theme	spheroid	895:902	arg1	models					904:909	3D tumour spheroid models	885:909	3D tumour spheroid models	885:909	In in vitro anticancer assay, MC/5-FU exhibited enhanced selectivity and anticancer actions against 2D monolayer and 3D tumour spheroid models colorectal cancer cells.
34560940	4	8	theme	scanning	527:534	arg1	microscopy					545:554	Transmission and scanning electron microscopy	510:554	microscopy	545:554	Transmission and scanning electron microscopy study demonstrated that Fe3O4 nanofillers covered the cellulose matrix.
34560940	6	9	theme	anticancer	841:850	arg1	actions					852:858	anticancer actions	841:858	anticancer actions	841:858	In in vitro anticancer assay, MC/5-FU exhibited enhanced selectivity and anticancer actions against 2D monolayer and 3D tumour spheroid models colorectal cancer cells.
34560940	1	10	theme	assorted	176:183	arg1	actions					200:206	assorted physiochemical actions	176:206	assorted physiochemical actions for applications	176:223	Magnetic polymer nanocomposites are inherently multifunctional and harbor assorted physiochemical actions for applications thereof as novel drug nanocarriers.
34560940	1	10	theme	assorted	176:183	arg1	nanocarriers					247:258	novel drug nanocarriers	236:258	novel drug nanocarriers	236:258	Magnetic polymer nanocomposites are inherently multifunctional and harbor assorted physiochemical actions for applications thereof as novel drug nanocarriers.
34560940	6	11	theme	3D	885:886	arg1	models					904:909	3D tumour spheroid models	885:909	3D tumour spheroid models	885:909	In in vitro anticancer assay, MC/5-FU exhibited enhanced selectivity and anticancer actions against 2D monolayer and 3D tumour spheroid models colorectal cancer cells.
34560940	8	12	theme	drug	1118:1121	arg1	formulation					1123:1133	a low-cost drug formulation	1107:1133	a low-cost drug formulation for colorectal cancer treatments	1107:1166	This easily synthesized MC/5-FU indicated the potential in application as a low-cost drug formulation for colorectal cancer treatments.
34560940	5	13	theme	drug	632:635	arg1	release					637:643	The drug release	628:643	The drug release from MC/5-FU	628:656	The drug release from MC/5-FU was evaluated under various pH and temperature conditions, showing the maximum release at pH 7.4 and 44.2 °C.
34560940	1	14	theme	Magnetic	102:109	arg1	nanocomposites					119:132	Magnetic polymer nanocomposites	102:132	Magnetic polymer nanocomposites	102:132	Magnetic polymer nanocomposites are inherently multifunctional and harbor assorted physiochemical actions for applications thereof as novel drug nanocarriers.
34560940	3	15	theme	MC/5-FU	483:489	arg1	bionanocomposites					491:507	MC/5-FU bionanocomposites	483:507	MC/5-FU bionanocomposites	483:507	Several analyses indicated the multifunctional properties of MC/5-FU bionanocomposites.
34560940	7	16	theme	anticancer	940:949	arg1	effects					951:957	The anticancer effects	936:957	The anticancer effects of MC/5-FU with magnetic targeting and heat induction	936:1011	The anticancer effects of MC/5-FU with magnetic targeting and heat induction were also examined.
34560940	1	17	theme	polymer	111:117	arg1	nanocomposites					119:132	Magnetic polymer nanocomposites	102:132	Magnetic polymer nanocomposites	102:132	Magnetic polymer nanocomposites are inherently multifunctional and harbor assorted physiochemical actions for applications thereof as novel drug nanocarriers.
34560940	7	18	theme	heat	998:1001	arg1	induction					1003:1011	heat induction	998:1011	heat induction	998:1011	The anticancer effects of MC/5-FU with magnetic targeting and heat induction were also examined.
34560940	1	19	theme	novel	236:240	arg1	actions					200:206	assorted physiochemical actions	176:206	assorted physiochemical actions for applications	176:223	Magnetic polymer nanocomposites are inherently multifunctional and harbor assorted physiochemical actions for applications thereof as novel drug nanocarriers.
34560940	1	19	theme	novel	236:240	arg1	nanocarriers					247:258	novel drug nanocarriers	236:258	novel drug nanocarriers	236:258	Magnetic polymer nanocomposites are inherently multifunctional and harbor assorted physiochemical actions for applications thereof as novel drug nanocarriers.
34560940	3	20	theme	bionanocomposites	491:507	arg1	properties					469:478	the multifunctional properties	449:478	the multifunctional properties of MC/5-FU bionanocomposites	449:507	Several analyses indicated the multifunctional properties of MC/5-FU bionanocomposites.
34560940	6	21	theme	cancer	922:927	arg1	cells					929:933	colorectal cancer cells	911:933	colorectal cancer cells	911:933	In in vitro anticancer assay, MC/5-FU exhibited enhanced selectivity and anticancer actions against 2D monolayer and 3D tumour spheroid models colorectal cancer cells.
34560940	2	22	theme	potential	382:390	arg1	treatments					410:419	potential colorectal cancer treatments	382:419	potential colorectal cancer treatments	382:419	Herein, Fe3O4-nanoparticles were supported on rice straw cellulose for 5-fluorouracil carrier abbreviated as MC/5-FU for potential colorectal cancer treatments.
34560940	6	23	theme	enhanced	816:823	arg1	selectivity					825:835	enhanced selectivity	816:835	enhanced selectivity	816:835	In in vitro anticancer assay, MC/5-FU exhibited enhanced selectivity and anticancer actions against 2D monolayer and 3D tumour spheroid models colorectal cancer cells.
34560940	1	24	theme	drug	242:245	arg1	actions					200:206	assorted physiochemical actions	176:206	assorted physiochemical actions for applications	176:223	Magnetic polymer nanocomposites are inherently multifunctional and harbor assorted physiochemical actions for applications thereof as novel drug nanocarriers.
34560940	1	24	theme	drug	242:245	arg1	nanocarriers					247:258	novel drug nanocarriers	236:258	novel drug nanocarriers	236:258	Magnetic polymer nanocomposites are inherently multifunctional and harbor assorted physiochemical actions for applications thereof as novel drug nanocarriers.
34560940	0	25	theme	cellulose	31:39	arg1	bionanocomposites					41:57	magnetic cellulose bionanocomposites	22:57	magnetic cellulose bionanocomposites	22:57	5-Fluorouracil loaded magnetic cellulose bionanocomposites for potential colorectal cancer treatment.
34560940	7	26	with	effects	951:957	arg1	targeting					984:992	magnetic targeting	975:992	magnetic targeting	975:992	The anticancer effects of MC/5-FU with magnetic targeting and heat induction were also examined.
34560940	7	26	with	effects	951:957	arg1	induction					1003:1011	heat induction	998:1011	heat induction	998:1011	The anticancer effects of MC/5-FU with magnetic targeting and heat induction were also examined.
34560940	6	27	theme	colorectal	911:920	arg1	cells					929:933	colorectal cancer cells	911:933	colorectal cancer cells	911:933	In in vitro anticancer assay, MC/5-FU exhibited enhanced selectivity and anticancer actions against 2D monolayer and 3D tumour spheroid models colorectal cancer cells.
34560940	5	28	theme	maximum	729:735	arg1	release					737:743	the maximum release	725:743	the maximum release at pH 7.4 and 44.2 °C	725:765	The drug release from MC/5-FU was evaluated under various pH and temperature conditions, showing the maximum release at pH 7.4 and 44.2 °C.
34560940	0	29	theme	magnetic	22:29	arg1	bionanocomposites					41:57	magnetic cellulose bionanocomposites	22:57	magnetic cellulose bionanocomposites	22:57	5-Fluorouracil loaded magnetic cellulose bionanocomposites for potential colorectal cancer treatment.
34560940	4	30	theme	Fe3O4	580:584	arg1	nanofillers					586:596	Fe3O4 nanofillers	580:596	Fe3O4 nanofillers	580:596	Transmission and scanning electron microscopy study demonstrated that Fe3O4 nanofillers covered the cellulose matrix.
34560940	7	31	theme	MC/5-FU	962:968	arg1	effects					951:957	The anticancer effects	936:957	The anticancer effects of MC/5-FU with magnetic targeting and heat induction	936:1011	The anticancer effects of MC/5-FU with magnetic targeting and heat induction were also examined.
34560940	7	32	theme	magnetic	975:982	arg1	targeting					984:992	magnetic targeting	975:992	magnetic targeting	975:992	The anticancer effects of MC/5-FU with magnetic targeting and heat induction were also examined.
34560940	5	33	from	44.2 °C	759:765	arg1	release					737:743	the maximum release	725:743	the maximum release at pH 7.4 and 44.2 °C	725:765	The drug release from MC/5-FU was evaluated under various pH and temperature conditions, showing the maximum release at pH 7.4 and 44.2 °C.
34560940	6	34	dep	monolayer	871:879	arg1	cells					929:933	colorectal cancer cells	911:933	colorectal cancer cells	911:933	In in vitro anticancer assay, MC/5-FU exhibited enhanced selectivity and anticancer actions against 2D monolayer and 3D tumour spheroid models colorectal cancer cells.
34560940	5	35	theme	various	678:684	arg1	conditions					705:714	various pH and temperature conditions	678:714	various pH and temperature conditions	678:714	The drug release from MC/5-FU was evaluated under various pH and temperature conditions, showing the maximum release at pH 7.4 and 44.2 °C.
34560940	8	36	from	potential	1079:1087	arg1	application					1092:1102	application	1092:1102	application	1092:1102	This easily synthesized MC/5-FU indicated the potential in application as a low-cost drug formulation for colorectal cancer treatments.
34560940	0	37	theme	colorectal	73:82	arg1	treatment					91:99	potential colorectal cancer treatment	63:99	potential colorectal cancer treatment	63:99	5-Fluorouracil loaded magnetic cellulose bionanocomposites for potential colorectal cancer treatment.
34560940	0	38	theme	cancer	84:89	arg1	treatment					91:99	potential colorectal cancer treatment	63:99	potential colorectal cancer treatment	63:99	5-Fluorouracil loaded magnetic cellulose bionanocomposites for potential colorectal cancer treatment.
34560940	5	39	theme	pH	686:687	arg1	conditions					705:714	various pH and temperature conditions	678:714	various pH and temperature conditions	678:714	The drug release from MC/5-FU was evaluated under various pH and temperature conditions, showing the maximum release at pH 7.4 and 44.2 °C.
34560940	0	40	theme	potential	63:71	arg1	treatment					91:99	potential colorectal cancer treatment	63:99	potential colorectal cancer treatment	63:99	5-Fluorouracil loaded magnetic cellulose bionanocomposites for potential colorectal cancer treatment.
34560940	8	41	theme	synthesized	1045:1055	arg1	MC/5-FU					1057:1063	This easily synthesized MC/5-FU	1033:1063	This easily synthesized MC/5-FU	1033:1063	This easily synthesized MC/5-FU indicated the potential in application as a low-cost drug formulation for colorectal cancer treatments.
34560940	6	42	theme	tumour	888:893	arg1	models					904:909	3D tumour spheroid models	885:909	3D tumour spheroid models	885:909	In in vitro anticancer assay, MC/5-FU exhibited enhanced selectivity and anticancer actions against 2D monolayer and 3D tumour spheroid models colorectal cancer cells.
34560940	4	43	theme	electron	536:543	arg1	microscopy					545:554	Transmission and scanning electron microscopy	510:554	microscopy	545:554	Transmission and scanning electron microscopy study demonstrated that Fe3O4 nanofillers covered the cellulose matrix.
34560940	5	44	theme	temperature	693:703	arg1	conditions					705:714	various pH and temperature conditions	678:714	various pH and temperature conditions	678:714	The drug release from MC/5-FU was evaluated under various pH and temperature conditions, showing the maximum release at pH 7.4 and 44.2 °C.
34560940	8	45	theme	colorectal	1139:1148	arg1	treatments					1157:1166	colorectal cancer treatments	1139:1166	colorectal cancer treatments	1139:1166	This easily synthesized MC/5-FU indicated the potential in application as a low-cost drug formulation for colorectal cancer treatments.
34560940	6	46	theme	anticancer	780:789	arg1	assay					791:795	in vitro anticancer assay	771:795	in vitro anticancer assay	771:795	In in vitro anticancer assay, MC/5-FU exhibited enhanced selectivity and anticancer actions against 2D monolayer and 3D tumour spheroid models colorectal cancer cells.
34560940	2	47	theme	5-fluorouracil	332:345	arg1	carrier					347:353	5-fluorouracil carrier	332:353	5-fluorouracil carrier abbreviated as MC/5-FU for potential colorectal cancer treatments	332:419	Herein, Fe3O4-nanoparticles were supported on rice straw cellulose for 5-fluorouracil carrier abbreviated as MC/5-FU for potential colorectal cancer treatments.
34560940	2	48	theme	cancer	403:408	arg1	treatments					410:419	potential colorectal cancer treatments	382:419	potential colorectal cancer treatments	382:419	Herein, Fe3O4-nanoparticles were supported on rice straw cellulose for 5-fluorouracil carrier abbreviated as MC/5-FU for potential colorectal cancer treatments.
34560940	4	49	dep	study	556:560	arg1	demonstrated					562:573	demonstrated	562:573	study demonstrated that Fe3O4 nanofillers covered the cellulose matrix	556:625	Transmission and scanning electron microscopy study demonstrated that Fe3O4 nanofillers covered the cellulose matrix.
34560940	2	50	theme	straw	312:316	arg1	cellulose					318:326	rice straw cellulose	307:326	rice straw cellulose for 5-fluorouracil carrier abbreviated as MC/5-FU for potential colorectal cancer treatments	307:419	Herein, Fe3O4-nanoparticles were supported on rice straw cellulose for 5-fluorouracil carrier abbreviated as MC/5-FU for potential colorectal cancer treatments.
34560940	6	51	dep	in	771:772	arg1	vitro					774:778	vitro	774:778	vitro	774:778	In in vitro anticancer assay, MC/5-FU exhibited enhanced selectivity and anticancer actions against 2D monolayer and 3D tumour spheroid models colorectal cancer cells.
34560940	1	52	theme	physiochemical	185:198	arg1	actions					200:206	assorted physiochemical actions	176:206	assorted physiochemical actions for applications	176:223	Magnetic polymer nanocomposites are inherently multifunctional and harbor assorted physiochemical actions for applications thereof as novel drug nanocarriers.
34560940	1	52	theme	physiochemical	185:198	arg1	nanocarriers					247:258	novel drug nanocarriers	236:258	novel drug nanocarriers	236:258	Magnetic polymer nanocomposites are inherently multifunctional and harbor assorted physiochemical actions for applications thereof as novel drug nanocarriers.
34560940	6	53	theme	in	771:772	arg1	assay					791:795	in vitro anticancer assay	771:795	in vitro anticancer assay	771:795	In in vitro anticancer assay, MC/5-FU exhibited enhanced selectivity and anticancer actions against 2D monolayer and 3D tumour spheroid models colorectal cancer cells.
34560940	3	54	theme	Several	422:428	arg1	analyses					430:437	Several analyses	422:437	Several analyses	422:437	Several analyses indicated the multifunctional properties of MC/5-FU bionanocomposites.
34560940	2	55	theme	rice	307:310	arg1	cellulose					318:326	rice straw cellulose	307:326	rice straw cellulose for 5-fluorouracil carrier abbreviated as MC/5-FU for potential colorectal cancer treatments	307:419	Herein, Fe3O4-nanoparticles were supported on rice straw cellulose for 5-fluorouracil carrier abbreviated as MC/5-FU for potential colorectal cancer treatments.
34560940	6	56	theme	2D	868:869	arg1	monolayer					871:879	2D monolayer	868:879	2D monolayer	868:879	In in vitro anticancer assay, MC/5-FU exhibited enhanced selectivity and anticancer actions against 2D monolayer and 3D tumour spheroid models colorectal cancer cells.
34560940	5	57	from	pH 7.4	748:753	arg1	release					737:743	the maximum release	725:743	the maximum release at pH 7.4 and 44.2 °C	725:765	The drug release from MC/5-FU was evaluated under various pH and temperature conditions, showing the maximum release at pH 7.4 and 44.2 °C.
32123275	9	0	theme	further	1805:1811	arg1	development					1813:1823	further development	1805:1823	further development in lignocellulose deconstruction applications	1805:1869	These characteristics suggest that Xy110E may be a promising candidate for further development in lignocellulose deconstruction applications.
32123275	1	1	theme	higher	265:270	arg1	fulviceps					333:341	fulviceps	333:341	fulviceps	333:341	In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus.
32123275	1	1	theme	higher	265:270	arg1	aquilinus					360:368	aquilinus	360:368	aquilinus	360:368	In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus.
32123275	1	1	theme	higher	265:270	arg1	species					280:286	higher termite species	265:286	higher termite species with different feeding habits	265:316	In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus.
32123275	6	2	theme	predicted	1244:1252	arg1	categories					1254:1263	the predicted categories	1240:1263	the predicted categories of the enzymes present in the gut microbiomes of each species	1240:1325	These findings suggest an association between the primary food source and the predicted categories of the enzymes present in the gut microbiomes of each species.
32123275	1	3	theme	microbial	161:169	arg1	potential					181:189	the microbial metabolic potential	157:189	the microbial metabolic potential for lignocellulose transformation	157:223	In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus.
32123275	4	4	theme	N.	898:899	arg1	aquilinus					901:909	N. aquilinus	898:909	N. aquilinus (a wood-feeder) gut microbiome samples	898:948	However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation.
32123275	4	4	theme	N.	898:899	arg1	wood-feeder					914:924	a wood-feeder	912:924	a wood-feeder	912:924	However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation.
32123275	6	5	attach	present	1280:1286	arg1	microbiomes					1299:1309	the gut microbiomes	1291:1309	the gut microbiomes of each species	1291:1325	These findings suggest an association between the primary food source and the predicted categories of the enzymes present in the gut microbiomes of each species.
32123275	6	5	attach	present	1280:1286	arg2	enzymes					1272:1278	the enzymes	1268:1278	the enzymes present in the gut microbiomes of each species	1268:1325	These findings suggest an association between the primary food source and the predicted categories of the enzymes present in the gut microbiomes of each species.
32123275	1	6	dep	Argentine	255:263	arg1	fulviceps					333:341	fulviceps	333:341	fulviceps	333:341	In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus.
32123275	1	6	dep	Argentine	255:263	arg1	aquilinus					360:368	aquilinus	360:368	aquilinus	360:368	In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus.
32123275	1	6	dep	Argentine	255:263	arg1	species					280:286	higher termite species	265:286	higher termite species with different feeding habits	265:316	In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus.
32123275	1	7	with	species	280:286	arg1	habits					311:316	different feeding habits	293:316	different feeding habits	293:316	In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus.
32123275	6	8	theme	food	1224:1227	arg1	source					1229:1234	the primary food source	1212:1234	the primary food source	1212:1234	These findings suggest an association between the primary food source and the predicted categories of the enzymes present in the gut microbiomes of each species.
32123275	5	9	theme	debranching-	1114:1125	arg1	enzymes					1157:1163	debranching- and oligosaccharide-degrading enzymes	1114:1163	debranching- and oligosaccharide-degrading enzymes	1114:1163	The C. fulviceps gut microbiome was enriched specifically in genes coding for debranching- and oligosaccharide-degrading enzymes.
32123275	3	10	theme	bacterial	588:596	arg1	phyla					598:602	the same five dominant bacterial phyla	565:602	the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances	565:722	Individuals from both termite species contained the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances.
32123275	1	11	theme	lignocellulose	195:208	arg1	transformation					210:223	lignocellulose transformation	195:223	lignocellulose transformation	195:223	In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus.
32123275	8	12	theme	Functional	1529:1538	arg1	analysis					1540:1547	Functional analysis	1529:1547	Functional analysis of the recombinant metagenome-derived enzyme	1529:1592	Functional analysis of the recombinant metagenome-derived enzyme showed high specificity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50 °C and a pH-activity range from 5 to 10.
32123275	5	13	theme	oligosaccharide-degrading	1131:1155	arg1	enzymes					1157:1163	debranching- and oligosaccharide-degrading enzymes	1114:1163	debranching- and oligosaccharide-degrading enzymes	1114:1163	The C. fulviceps gut microbiome was enriched specifically in genes coding for debranching- and oligosaccharide-degrading enzymes.
32123275	4	14	theme	gut	927:929	arg1	samples					942:948	N. aquilinus (a wood-feeder) gut microbiome samples	898:948	N. aquilinus (a wood-feeder) gut microbiome samples	898:948	However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation.
32123275	6	15	theme	gut	1295:1297	arg1	microbiomes					1299:1309	the gut microbiomes	1291:1309	the gut microbiomes of each species	1291:1325	These findings suggest an association between the primary food source and the predicted categories of the enzymes present in the gut microbiomes of each species.
32123275	8	16	theme	recombinant	1556:1566	arg1	enzyme					1587:1592	the recombinant metagenome-derived enzyme	1552:1592	the recombinant metagenome-derived enzyme	1552:1592	Functional analysis of the recombinant metagenome-derived enzyme showed high specificity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50 °C and a pH-activity range from 5 to 10.
32123275	7	17	theme	putative	1440:1447	arg1	Xyl10E					1475:1480	Xyl10E	1475:1480	Xyl10E	1475:1480	To further investigate the termite microbiomes as sources of biotechnologically relevant glycosyl hydrolases, a putative GH10 endo-β-1,4-xylanase, Xyl10E, was cloned and expressed in Escherichia coli.
32123275	7	17	theme	putative	1440:1447	arg1	endo-β-1,4-xylanase					1454:1472	a putative GH10 endo-β-1,4-xylanase	1438:1472	a putative GH10 endo-β-1,4-xylanase	1438:1472	To further investigate the termite microbiomes as sources of biotechnologically relevant glycosyl hydrolases, a putative GH10 endo-β-1,4-xylanase, Xyl10E, was cloned and expressed in Escherichia coli.
32123275	1	18	theme	shotgun	110:116	arg1	sequencing					130:139	shotgun metagenomic sequencing	110:139	shotgun metagenomic sequencing	110:139	In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus.
32123275	4	19	theme	cellulose	1013:1021	arg1	degradation					1023:1033	cellulose degradation	1013:1033	cellulose degradation	1013:1033	However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation.
32123275	8	20	theme	enzyme	1587:1592	arg1	analysis					1540:1547	Functional analysis	1529:1547	Functional analysis of the recombinant metagenome-derived enzyme	1529:1592	Functional analysis of the recombinant metagenome-derived enzyme showed high specificity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50 °C and a pH-activity range from 5 to 10.
32123275	6	21	theme	enzymes	1272:1278	arg1	source					1229:1234	the primary food source	1212:1234	the primary food source	1212:1234	These findings suggest an association between the primary food source and the predicted categories of the enzymes present in the gut microbiomes of each species.
32123275	6	21	theme	enzymes	1272:1278	arg1	categories					1254:1263	the predicted categories	1240:1263	the predicted categories of the enzymes present in the gut microbiomes of each species	1240:1325	These findings suggest an association between the primary food source and the predicted categories of the enzymes present in the gut microbiomes of each species.
32123275	6	22	from	microbiomes	1299:1309	arg1	present					1280:1286	present	1280:1286	present	1280:1286	These findings suggest an association between the primary food source and the predicted categories of the enzymes present in the gut microbiomes of each species.
32123275	0	23	theme	cell	58:61	arg1	enzymes					78:84	novel plant cell wall degrading enzymes	46:84	novel plant cell wall degrading enzymes	46:84	Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes.
32123275	8	24	dep	10	1726:1727	arg1	to					1723:1724	to	1723:1724	to	1723:1724	Functional analysis of the recombinant metagenome-derived enzyme showed high specificity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50 °C and a pH-activity range from 5 to 10.
32123275	9	25	from	development	1813:1823	arg1	applications					1858:1869	lignocellulose deconstruction applications	1828:1869	lignocellulose deconstruction applications	1828:1869	These characteristics suggest that Xy110E may be a promising candidate for further development in lignocellulose deconstruction applications.
32123275	8	26	theme	high	1601:1604	arg1	specificity					1606:1616	high specificity	1601:1616	high specificity towards beechwood xylan (288.1 IU/mg)	1601:1654	Functional analysis of the recombinant metagenome-derived enzyme showed high specificity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50 °C and a pH-activity range from 5 to 10.
32123275	9	27	theme	lignocellulose	1828:1841	arg1	applications					1858:1869	lignocellulose deconstruction applications	1828:1869	lignocellulose deconstruction applications	1828:1869	These characteristics suggest that Xy110E may be a promising candidate for further development in lignocellulose deconstruction applications.
32123275	4	28	theme	functional	743:752	arg1	capacity					754:761	detected functional capacity	734:761	detected functional capacity	734:761	However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation.
32123275	0	29	theme	degrading	68:76	arg1	enzymes					78:84	novel plant cell wall degrading enzymes	46:84	novel plant cell wall degrading enzymes	46:84	Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes.
32123275	7	30	theme	GH10	1449:1452	arg1	Xyl10E					1475:1480	Xyl10E	1475:1480	Xyl10E	1475:1480	To further investigate the termite microbiomes as sources of biotechnologically relevant glycosyl hydrolases, a putative GH10 endo-β-1,4-xylanase, Xyl10E, was cloned and expressed in Escherichia coli.
32123275	7	30	theme	GH10	1449:1452	arg1	endo-β-1,4-xylanase					1454:1472	a putative GH10 endo-β-1,4-xylanase	1438:1472	a putative GH10 endo-β-1,4-xylanase	1438:1472	To further investigate the termite microbiomes as sources of biotechnologically relevant glycosyl hydrolases, a putative GH10 endo-β-1,4-xylanase, Xyl10E, was cloned and expressed in Escherichia coli.
32123275	6	31	from	present	1280:1286	arg1	microbiomes					1299:1309	the gut microbiomes	1291:1309	the gut microbiomes of each species	1291:1325	These findings suggest an association between the primary food source and the predicted categories of the enzymes present in the gut microbiomes of each species.
32123275	4	32	theme	microbiome	815:824	arg1	samples					826:832	C. fulviceps (a grass-wood-feeder) gut microbiome samples	776:832	C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism	776:887	However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation.
32123275	2	33	theme	microbial	398:406	arg1	compositions					418:429	the microbial community compositions	394:429	the microbial community compositions	394:429	Our goal was to assess the microbial community compositions and metabolic capacity, and to identify genes involved in lignocellulose degradation.
32123275	7	34	theme	hydrolases	1426:1435	arg1	sources					1378:1384	sources	1378:1384	sources of biotechnologically relevant glycosyl hydrolases	1378:1435	To further investigate the termite microbiomes as sources of biotechnologically relevant glycosyl hydrolases, a putative GH10 endo-β-1,4-xylanase, Xyl10E, was cloned and expressed in Escherichia coli.
32123275	8	35	theme	288.1	1643:1647	arg1	xylan					1636:1640	beechwood xylan	1626:1640	beechwood xylan (288.1 IU/mg)	1626:1654	Functional analysis of the recombinant metagenome-derived enzyme showed high specificity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50 °C and a pH-activity range from 5 to 10.
32123275	8	35	theme	288.1	1643:1647	arg1	IU/mg					1649:1653	288.1 IU/mg	1643:1653	288.1 IU/mg	1643:1653	Functional analysis of the recombinant metagenome-derived enzyme showed high specificity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50 °C and a pH-activity range from 5 to 10.
32123275	9	36	theme	deconstruction	1843:1856	arg1	applications					1858:1869	lignocellulose deconstruction applications	1828:1869	lignocellulose deconstruction applications	1828:1869	These characteristics suggest that Xy110E may be a promising candidate for further development in lignocellulose deconstruction applications.
32123275	4	37	theme	fulviceps	779:787	arg1	samples					826:832	C. fulviceps (a grass-wood-feeder) gut microbiome samples	776:832	C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism	776:887	However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation.
32123275	1	38	theme	termite	272:278	arg1	fulviceps					333:341	fulviceps	333:341	fulviceps	333:341	In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus.
32123275	1	38	theme	termite	272:278	arg1	aquilinus					360:368	aquilinus	360:368	aquilinus	360:368	In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus.
32123275	1	38	theme	termite	272:278	arg1	species					280:286	higher termite species	265:286	higher termite species with different feeding habits	265:316	In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus.
32123275	7	39	theme	relevant	1408:1415	arg1	hydrolases					1426:1435	biotechnologically relevant glycosyl hydrolases	1389:1435	biotechnologically relevant glycosyl hydrolases	1389:1435	To further investigate the termite microbiomes as sources of biotechnologically relevant glycosyl hydrolases, a putative GH10 endo-β-1,4-xylanase, Xyl10E, was cloned and expressed in Escherichia coli.
32123275	2	40	theme	metabolic	435:443	arg1	capacity					445:452	metabolic capacity	435:452	metabolic capacity	435:452	Our goal was to assess the microbial community compositions and metabolic capacity, and to identify genes involved in lignocellulose degradation.
32123275	1	41	theme	feeding	303:309	arg1	habits					311:316	different feeding habits	293:316	different feeding habits	293:316	In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus.
32123275	8	42	theme	optimum	1666:1672	arg1	activity					1674:1681	the optimum activity	1662:1681	the optimum activity at 50 °C	1662:1690	Functional analysis of the recombinant metagenome-derived enzyme showed high specificity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50 °C and a pH-activity range from 5 to 10.
32123275	4	43	theme	acid	873:876	arg1	metabolism					878:887	amino acid metabolism	867:887	amino acid metabolism	867:887	However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation.
32123275	0	44	theme	novel	46:50	arg1	enzymes					78:84	novel plant cell wall degrading enzymes	46:84	novel plant cell wall degrading enzymes	46:84	Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes.
32123275	7	45	theme	termite	1355:1361	arg1	microbiomes					1363:1373	the termite microbiomes	1351:1373	the termite microbiomes as sources of biotechnologically relevant glycosyl hydrolases	1351:1435	To further investigate the termite microbiomes as sources of biotechnologically relevant glycosyl hydrolases, a putative GH10 endo-β-1,4-xylanase, Xyl10E, was cloned and expressed in Escherichia coli.
32123275	5	46	theme	C.	1040:1041	arg1	microbiome					1057:1066	The C. fulviceps gut microbiome	1036:1066	The C. fulviceps gut microbiome	1036:1066	The C. fulviceps gut microbiome was enriched specifically in genes coding for debranching- and oligosaccharide-degrading enzymes.
32123275	3	47	theme	relative	704:711	arg1	abundances					713:722	different relative abundances	694:722	the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances	565:722	Individuals from both termite species contained the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances.
32123275	3	48	theme	termite	539:545	arg1	species					547:553	both termite species	534:553	both termite species	534:553	Individuals from both termite species contained the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances.
32123275	3	49	dep	although	680:687	arg1	with					689:692	with	689:692	with	689:692	Individuals from both termite species contained the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances.
32123275	5	50	theme	gut	1053:1055	arg1	microbiome					1057:1066	The C. fulviceps gut microbiome	1036:1066	The C. fulviceps gut microbiome	1036:1066	The C. fulviceps gut microbiome was enriched specifically in genes coding for debranching- and oligosaccharide-degrading enzymes.
32123275	8	51	theme	pH-activity	1698:1708	arg1	range					1710:1714	a pH-activity range	1696:1714	a pH-activity range from 5 to 10	1696:1727	Functional analysis of the recombinant metagenome-derived enzyme showed high specificity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50 °C and a pH-activity range from 5 to 10.
32123275	1	52	theme	colonies	243:250	arg1	gut					232:234	the gut	228:234	the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus	228:368	In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus.
32123275	4	53	theme	aquilinus	901:909	arg1	samples					942:948	N. aquilinus (a wood-feeder) gut microbiome samples	898:948	N. aquilinus (a wood-feeder) gut microbiome samples	898:948	However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation.
32123275	3	54	theme	same	569:572	arg1	phyla					598:602	the same five dominant bacterial phyla	565:602	the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances	565:722	Individuals from both termite species contained the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances.
32123275	1	55	theme	Argentine	255:263	arg1	colonies					243:250	two colonies	239:250	two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus	239:368	In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus.
32123275	8	56	from	50 °C	1686:1690	arg1	activity					1674:1681	the optimum activity	1662:1681	the optimum activity at 50 °C	1662:1690	Functional analysis of the recombinant metagenome-derived enzyme showed high specificity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50 °C and a pH-activity range from 5 to 10.
32123275	8	56	from	50 °C	1686:1690	arg1	range					1710:1714	a pH-activity range	1696:1714	a pH-activity range from 5 to 10	1696:1727	Functional analysis of the recombinant metagenome-derived enzyme showed high specificity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50 °C and a pH-activity range from 5 to 10.
32123275	3	57	from	species	547:553	arg1	Individuals					517:527	Individuals	517:527	Individuals from both termite species	517:553	Individuals from both termite species contained the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances.
32123275	6	58	theme	species	1319:1325	arg1	microbiomes					1299:1309	the gut microbiomes	1291:1309	the gut microbiomes of each species	1291:1325	These findings suggest an association between the primary food source and the predicted categories of the enzymes present in the gut microbiomes of each species.
32123275	1	59	theme	metabolic	171:179	arg1	potential					181:189	the microbial metabolic potential	157:189	the microbial metabolic potential for lignocellulose transformation	157:223	In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus.
32123275	1	60	used	used	105:108	arg2	we					102:103	we	102:103	we	102:103	In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus.
32123275	3	61	theme	dominant	579:586	arg1	phyla					598:602	the same five dominant bacterial phyla	565:602	the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances	565:722	Individuals from both termite species contained the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances.
32123275	9	62	theme	promising	1781:1789	arg1	candidate					1791:1799	a promising candidate	1779:1799	a promising candidate for further development in lignocellulose deconstruction applications	1779:1869	These characteristics suggest that Xy110E may be a promising candidate for further development in lignocellulose deconstruction applications.
32123275	9	62	theme	promising	1781:1789	arg1	Xy110E					1765:1770	Xy110E	1765:1770	Xy110E	1765:1770	These characteristics suggest that Xy110E may be a promising candidate for further development in lignocellulose deconstruction applications.
32123275	3	63	contain	contained	555:563	arg1	Individuals					517:527	Individuals	517:527	Individuals from both termite species	517:553	Individuals from both termite species contained the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances.
32123275	3	63	contain	contained	555:563	arg2	abundances					713:722	different relative abundances	694:722	the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances	565:722	Individuals from both termite species contained the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances.
32123275	3	63	contain	contained	555:563	arg2	phyla					598:602	the same five dominant bacterial phyla	565:602	the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances	565:722	Individuals from both termite species contained the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances.
32123275	6	64	theme	primary	1216:1222	arg1	source					1229:1234	the primary food source	1212:1234	the primary food source	1212:1234	These findings suggest an association between the primary food source and the predicted categories of the enzymes present in the gut microbiomes of each species.
32123275	8	65	from	10	1726:1727	arg1	activity					1674:1681	the optimum activity	1662:1681	the optimum activity at 50 °C	1662:1690	Functional analysis of the recombinant metagenome-derived enzyme showed high specificity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50 °C and a pH-activity range from 5 to 10.
32123275	8	65	from	10	1726:1727	arg1	range					1710:1714	a pH-activity range	1696:1714	a pH-activity range from 5 to 10	1696:1727	Functional analysis of the recombinant metagenome-derived enzyme showed high specificity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50 °C and a pH-activity range from 5 to 10.
32123275	8	66	theme	metagenome-derived	1568:1585	arg1	enzyme					1587:1592	the recombinant metagenome-derived enzyme	1552:1592	the recombinant metagenome-derived enzyme	1552:1592	Functional analysis of the recombinant metagenome-derived enzyme showed high specificity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50 °C and a pH-activity range from 5 to 10.
32123275	4	67	theme	microbiome	931:940	arg1	samples					942:948	N. aquilinus (a wood-feeder) gut microbiome samples	898:948	N. aquilinus (a wood-feeder) gut microbiome samples	898:948	However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation.
32123275	6	68	theme	present	1280:1286	arg1	enzymes					1272:1278	the enzymes	1268:1278	the enzymes present in the gut microbiomes of each species	1268:1325	These findings suggest an association between the primary food source and the predicted categories of the enzymes present in the gut microbiomes of each species.
32123275	0	69	theme	wall	63:66	arg1	enzymes					78:84	novel plant cell wall degrading enzymes	46:84	novel plant cell wall degrading enzymes	46:84	Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes.
32123275	1	70	theme	metagenomic	118:128	arg1	sequencing					130:139	shotgun metagenomic sequencing	110:139	shotgun metagenomic sequencing	110:139	In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus.
32123275	4	71	contain	containing	834:843	arg1	samples					826:832	C. fulviceps (a grass-wood-feeder) gut microbiome samples	776:832	C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism	776:887	However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation.
32123275	4	71	contain	containing	834:843	arg2	genes					850:854	more genes	845:854	more genes related to amino acid metabolism	845:887	However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation.
32123275	0	72	theme	enzymes	78:84	arg1	sources					35:41	sources	35:41	sources of novel plant cell wall degrading enzymes	35:84	Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes.
32123275	3	73	dep	phyla	598:602	arg1	Firmicutes					619:628	Firmicutes	619:628	Firmicutes	619:628	Individuals from both termite species contained the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances.
32123275	3	73	dep	phyla	598:602	arg1	Spirochaetes					605:616	Spirochaetes	605:616	Spirochaetes	605:616	Individuals from both termite species contained the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances.
32123275	3	73	dep	phyla	598:602	arg1	Fibrobacteres					647:659	Fibrobacteres	647:659	Fibrobacteres	647:659	Individuals from both termite species contained the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances.
32123275	3	73	dep	phyla	598:602	arg1	Bacteroidetes					665:677	Bacteroidetes	665:677	Bacteroidetes	665:677	Individuals from both termite species contained the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances.
32123275	3	73	dep	phyla	598:602	arg1	Proteobacteria					631:644	Proteobacteria	631:644	Proteobacteria	631:644	Individuals from both termite species contained the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances.
32123275	2	74	theme	lignocellulose	489:502	arg1	degradation					504:514	lignocellulose degradation	489:514	lignocellulose degradation	489:514	Our goal was to assess the microbial community compositions and metabolic capacity, and to identify genes involved in lignocellulose degradation.
32123275	8	75	theme	beechwood	1626:1634	arg1	xylan					1636:1640	beechwood xylan	1626:1640	beechwood xylan (288.1 IU/mg)	1626:1654	Functional analysis of the recombinant metagenome-derived enzyme showed high specificity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50 °C and a pH-activity range from 5 to 10.
32123275	8	75	theme	beechwood	1626:1634	arg1	IU/mg					1649:1653	288.1 IU/mg	1643:1653	288.1 IU/mg	1643:1653	Functional analysis of the recombinant metagenome-derived enzyme showed high specificity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50 °C and a pH-activity range from 5 to 10.
32123275	4	76	theme	detected	734:741	arg1	capacity					754:761	detected functional capacity	734:761	detected functional capacity	734:761	However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation.
32123275	7	77	theme	glycosyl	1417:1424	arg1	hydrolases					1426:1435	biotechnologically relevant glycosyl hydrolases	1389:1435	biotechnologically relevant glycosyl hydrolases	1389:1435	To further investigate the termite microbiomes as sources of biotechnologically relevant glycosyl hydrolases, a putative GH10 endo-β-1,4-xylanase, Xyl10E, was cloned and expressed in Escherichia coli.
32123275	4	78	theme	carbohydrate	985:996	arg1	metabolism					998:1007	carbohydrate metabolism	985:1007	carbohydrate metabolism	985:1007	However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation.
32123275	4	79	theme	gut	811:813	arg1	samples					826:832	C. fulviceps (a grass-wood-feeder) gut microbiome samples	776:832	C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism	776:887	However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation.
32123275	4	80	theme	C.	776:777	arg1	fulviceps					779:787	C. fulviceps	776:787	C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism	776:887	However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation.
32123275	4	80	theme	C.	776:777	arg1	grass-wood-feeder					792:808	a grass-wood-feeder	790:808	a grass-wood-feeder	790:808	However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation.
32123275	1	81	theme	different	293:301	arg1	habits					311:316	different feeding habits	293:316	different feeding habits	293:316	In this study, we used shotgun metagenomic sequencing to characterise the microbial metabolic potential for lignocellulose transformation in the gut of two colonies of Argentine higher termite species with different feeding habits, Cortaritermes fulviceps and Nasutitermes aquilinus.
32123275	8	82	link	metagenome-derived	1568:1585	arg1	enzyme					1587:1592	the recombinant metagenome-derived enzyme	1552:1592	the recombinant metagenome-derived enzyme	1552:1592	Functional analysis of the recombinant metagenome-derived enzyme showed high specificity towards beechwood xylan (288.1 IU/mg), with the optimum activity at 50 °C and a pH-activity range from 5 to 10.
32123275	4	83	theme	related	856:862	arg1	genes					850:854	more genes	845:854	more genes related to amino acid metabolism	845:887	However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation.
32123275	2	84	theme	community	408:416	arg1	compositions					418:429	the microbial community compositions	394:429	the microbial community compositions	394:429	Our goal was to assess the microbial community compositions and metabolic capacity, and to identify genes involved in lignocellulose degradation.
32123275	0	85	theme	plant	52:56	arg1	enzymes					78:84	novel plant cell wall degrading enzymes	46:84	novel plant cell wall degrading enzymes	46:84	Neotropical termite microbiomes as sources of novel plant cell wall degrading enzymes.
32123275	4	86	theme	amino	867:871	arg1	metabolism					878:887	amino acid metabolism	867:887	amino acid metabolism	867:887	However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation.
32123275	3	87	theme	different	694:702	arg1	abundances					713:722	different relative abundances	694:722	the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances	565:722	Individuals from both termite species contained the same five dominant bacterial phyla (Spirochaetes, Firmicutes, Proteobacteria, Fibrobacteres and Bacteroidetes) although with different relative abundances.
32123275	5	88	theme	fulviceps	1043:1051	arg1	microbiome					1057:1066	The C. fulviceps gut microbiome	1036:1066	The C. fulviceps gut microbiome	1036:1066	The C. fulviceps gut microbiome was enriched specifically in genes coding for debranching- and oligosaccharide-degrading enzymes.
32123275	4	89	theme	more	845:848	arg1	genes					850:854	more genes	845:854	more genes related to amino acid metabolism	845:887	However, detected functional capacity varied, with C. fulviceps (a grass-wood-feeder) gut microbiome samples containing more genes related to amino acid metabolism, whereas N. aquilinus (a wood-feeder) gut microbiome samples were enriched in genes involved in carbohydrate metabolism and cellulose degradation.
34369649	0	0	theme	rodentium-Induced	92:108	arg1	Colitis					110:116	Citrobacter rodentium-Induced Colitis	80:116	Citrobacter rodentium-Induced Colitis	80:116	Pectins that Structurally Differ in the Distribution of Methyl-Esters Attenuate Citrobacter rodentium-Induced Colitis.
34369649	3	1	dep	model	708:712	arg1	induced					741:747	induced	741:747	induced colitis	741:755	Therefore, this study tests pectins that structurally differ in DB in a mouse model with Citrobacter rodentium induced colitis and studies the impact on the intestinal microbiota composition and associated attenuation of inflammation.
34369649	3	1	dep	model	708:712	arg1	studies					761:767	studies	761:767	studies the impact on the intestinal microbiota composition and associated attenuation of inflammation	761:862	Therefore, this study tests pectins that structurally differ in DB in a mouse model with Citrobacter rodentium induced colitis and studies the impact on the intestinal microbiota composition and associated attenuation of inflammation.
34369649	4	2	theme	high	898:901	arg1	pectins					906:912	Both low and high DB pectins	885:912	METHODS AND RESULTS Both low and high DB pectins	865:912	METHODS AND RESULTS Both low and high DB pectins induce a more rich and diverse microbiota composition.
34369649	7	3	theme	rodentium	1377:1385	arg1	inflammation					1403:1414	C. rodentium induced colonic inflammation	1374:1414	C. rodentium induced colonic inflammation	1374:1414	CONCLUSION Pectins prevent C. rodentium induced colonic inflammation by lowering the C. rodentium load in the caecum independently of the DB.
34369649	5	4	from	load	1008:1011	arg1	digesta					1038:1044	cecal digesta	1032:1044	cecal digesta	1032:1044	These pectins also lower the bacterial load of C. rodentium in cecal digesta.
34369649	0	5	theme	Citrobacter	80:90	arg1	Colitis					110:116	Citrobacter rodentium-Induced Colitis	80:116	Citrobacter rodentium-Induced Colitis	80:116	Pectins that Structurally Differ in the Distribution of Methyl-Esters Attenuate Citrobacter rodentium-Induced Colitis.
34369649	6	6	theme	induced	1252:1258	arg1	colitis					1260:1266	C. rodentium induced colitis	1239:1266	C. rodentium induced colitis	1239:1266	Through these effects, both low and high DB pectins attenuate C. rodentium induced colitis resulting in reduced intestinal damage, reduced numbers of Th1-cells, which are increased in case of C. rodentium induced colitis, and reduced levels of GATA3+ Tregs, which are related to tissue inflammation.
34369649	1	7	theme	direct	205:210	arg1	interactions					212:223	direct interactions	205:223	direct interactions on Toll-like receptors (TLRs) in the small intestine	205:276	INTRODUCTION Pectins have anti-inflammatory properties on intestinal immunity through direct interactions on Toll-like receptors (TLRs) in the small intestine or via stimulating microbiota-dependent effects in the large intestine.
34369649	1	8	theme	stimulating	285:295	arg1	effects					318:324	stimulating microbiota-dependent effects	285:324	stimulating microbiota-dependent effects in the large intestine	285:347	INTRODUCTION Pectins have anti-inflammatory properties on intestinal immunity through direct interactions on Toll-like receptors (TLRs) in the small intestine or via stimulating microbiota-dependent effects in the large intestine.
34369649	1	9	contain	have	140:143	arg2	properties					163:172	anti-inflammatory properties	145:172	anti-inflammatory properties	145:172	INTRODUCTION Pectins have anti-inflammatory properties on intestinal immunity through direct interactions on Toll-like receptors (TLRs) in the small intestine or via stimulating microbiota-dependent effects in the large intestine.
34369649	1	9	contain	have	140:143	arg1	Pectins					132:138	INTRODUCTION Pectins	119:138	INTRODUCTION Pectins	119:138	INTRODUCTION Pectins have anti-inflammatory properties on intestinal immunity through direct interactions on Toll-like receptors (TLRs) in the small intestine or via stimulating microbiota-dependent effects in the large intestine.
34369649	6	10	theme	reduced	1273:1279	arg1	levels					1281:1286	reduced levels	1273:1286	reduced levels of GATA3+ Tregs, which are related to tissue inflammation	1273:1344	Through these effects, both low and high DB pectins attenuate C. rodentium induced colitis resulting in reduced intestinal damage, reduced numbers of Th1-cells, which are increased in case of C. rodentium induced colitis, and reduced levels of GATA3+ Tregs, which are related to tissue inflammation.
34369649	6	11	theme	Th1-cells	1197:1205	arg1	damage					1170:1175	reduced intestinal damage	1151:1175	reduced intestinal damage	1151:1175	Through these effects, both low and high DB pectins attenuate C. rodentium induced colitis resulting in reduced intestinal damage, reduced numbers of Th1-cells, which are increased in case of C. rodentium induced colitis, and reduced levels of GATA3+ Tregs, which are related to tissue inflammation.
34369649	6	11	theme	Th1-cells	1197:1205	arg1	numbers					1186:1192	reduced numbers	1178:1192	reduced numbers of Th1-cells, which are increased in case of C. rodentium induced colitis	1178:1266	Through these effects, both low and high DB pectins attenuate C. rodentium induced colitis resulting in reduced intestinal damage, reduced numbers of Th1-cells, which are increased in case of C. rodentium induced colitis, and reduced levels of GATA3+ Tregs, which are related to tissue inflammation.
34369649	6	11	theme	Th1-cells	1197:1205	arg1	levels					1281:1286	reduced levels	1273:1286	reduced levels of GATA3+ Tregs, which are related to tissue inflammation	1273:1344	Through these effects, both low and high DB pectins attenuate C. rodentium induced colitis resulting in reduced intestinal damage, reduced numbers of Th1-cells, which are increased in case of C. rodentium induced colitis, and reduced levels of GATA3+ Tregs, which are related to tissue inflammation.
34369649	7	12	from	load	1445:1448	arg1	caecum					1457:1462	the caecum	1453:1462	the caecum independently of the DB	1453:1486	CONCLUSION Pectins prevent C. rodentium induced colonic inflammation by lowering the C. rodentium load in the caecum independently of the DB.
34369649	1	13	theme	microbiota-dependent	297:316	arg1	effects					318:324	stimulating microbiota-dependent effects	285:324	stimulating microbiota-dependent effects in the large intestine	285:347	INTRODUCTION Pectins have anti-inflammatory properties on intestinal immunity through direct interactions on Toll-like receptors (TLRs) in the small intestine or via stimulating microbiota-dependent effects in the large intestine.
34369649	4	14	theme	DB	903:904	arg1	pectins					906:912	Both low and high DB pectins	885:912	METHODS AND RESULTS Both low and high DB pectins	865:912	METHODS AND RESULTS Both low and high DB pectins induce a more rich and diverse microbiota composition.
34369649	7	15	theme	C.	1432:1433	arg1	load					1445:1448	the C. rodentium load	1428:1448	the C. rodentium load in the caecum independently of the DB	1428:1486	CONCLUSION Pectins prevent C. rodentium induced colonic inflammation by lowering the C. rodentium load in the caecum independently of the DB.
34369649	3	16	theme	inflammation	851:862	arg1	composition					809:819	intestinal microbiota composition	787:819	intestinal microbiota composition	787:819	Therefore, this study tests pectins that structurally differ in DB in a mouse model with Citrobacter rodentium induced colitis and studies the impact on the intestinal microbiota composition and associated attenuation of inflammation.
34369649	3	16	theme	inflammation	851:862	arg1	attenuation					836:846	associated attenuation	825:846	associated attenuation	825:846	Therefore, this study tests pectins that structurally differ in DB in a mouse model with Citrobacter rodentium induced colitis and studies the impact on the intestinal microbiota composition and associated attenuation of inflammation.
34369649	6	17	theme	C.	1239:1240	arg1	colitis					1260:1266	C. rodentium induced colitis	1239:1266	C. rodentium induced colitis	1239:1266	Through these effects, both low and high DB pectins attenuate C. rodentium induced colitis resulting in reduced intestinal damage, reduced numbers of Th1-cells, which are increased in case of C. rodentium induced colitis, and reduced levels of GATA3+ Tregs, which are related to tissue inflammation.
34369649	6	18	theme	Tregs	1298:1302	arg1	damage					1170:1175	reduced intestinal damage	1151:1175	reduced intestinal damage	1151:1175	Through these effects, both low and high DB pectins attenuate C. rodentium induced colitis resulting in reduced intestinal damage, reduced numbers of Th1-cells, which are increased in case of C. rodentium induced colitis, and reduced levels of GATA3+ Tregs, which are related to tissue inflammation.
34369649	6	18	theme	Tregs	1298:1302	arg1	numbers					1186:1192	reduced numbers	1178:1192	reduced numbers of Th1-cells, which are increased in case of C. rodentium induced colitis	1178:1266	Through these effects, both low and high DB pectins attenuate C. rodentium induced colitis resulting in reduced intestinal damage, reduced numbers of Th1-cells, which are increased in case of C. rodentium induced colitis, and reduced levels of GATA3+ Tregs, which are related to tissue inflammation.
34369649	6	18	theme	Tregs	1298:1302	arg1	levels					1281:1286	reduced levels	1273:1286	reduced levels of GATA3+ Tregs, which are related to tissue inflammation	1273:1344	Through these effects, both low and high DB pectins attenuate C. rodentium induced colitis resulting in reduced intestinal damage, reduced numbers of Th1-cells, which are increased in case of C. rodentium induced colitis, and reduced levels of GATA3+ Tregs, which are related to tissue inflammation.
34369649	5	19	theme	bacterial	998:1006	arg1	load					1008:1011	the bacterial load	994:1011	the bacterial load of C. rodentium in cecal digesta	994:1044	These pectins also lower the bacterial load of C. rodentium in cecal digesta.
34369649	2	20	theme	methyl-esters	420:432	arg1	distribution					404:415	the distribution	400:415	the distribution of methyl-esters (degree of blockiness; DB) of pectins	400:470	Both the degree of methyl-esterification (DM) and the distribution of methyl-esters (degree of blockiness; DB) of pectins contribute to this influence on immunity, but whether and how the DB impacts immunity through microbiota-dependent effects in the large intestine is unknown.
34369649	2	20	theme	methyl-esters	420:432	arg1	degree					359:364	the degree	355:364	the degree of methyl-esterification (DM)	355:394	Both the degree of methyl-esterification (DM) and the distribution of methyl-esters (degree of blockiness; DB) of pectins contribute to this influence on immunity, but whether and how the DB impacts immunity through microbiota-dependent effects in the large intestine is unknown.
34369649	6	21	theme	DB	1088:1089	arg1	pectins					1091:1097	both low and high DB pectins	1070:1097	both low and high DB pectins	1070:1097	Through these effects, both low and high DB pectins attenuate C. rodentium induced colitis resulting in reduced intestinal damage, reduced numbers of Th1-cells, which are increased in case of C. rodentium induced colitis, and reduced levels of GATA3+ Tregs, which are related to tissue inflammation.
34369649	3	22	theme	Citrobacter	719:729	arg1	rodentium					731:739	Citrobacter rodentium	719:739	Citrobacter rodentium	719:739	Therefore, this study tests pectins that structurally differ in DB in a mouse model with Citrobacter rodentium induced colitis and studies the impact on the intestinal microbiota composition and associated attenuation of inflammation.
34369649	6	23	theme	reduced	1178:1184	arg1	numbers					1186:1192	reduced numbers	1178:1192	reduced numbers of Th1-cells, which are increased in case of C. rodentium induced colitis	1178:1266	Through these effects, both low and high DB pectins attenuate C. rodentium induced colitis resulting in reduced intestinal damage, reduced numbers of Th1-cells, which are increased in case of C. rodentium induced colitis, and reduced levels of GATA3+ Tregs, which are related to tissue inflammation.
34369649	6	24	theme	high	1083:1086	arg1	pectins					1091:1097	both low and high DB pectins	1070:1097	both low and high DB pectins	1070:1097	Through these effects, both low and high DB pectins attenuate C. rodentium induced colitis resulting in reduced intestinal damage, reduced numbers of Th1-cells, which are increased in case of C. rodentium induced colitis, and reduced levels of GATA3+ Tregs, which are related to tissue inflammation.
34369649	2	25	from	influence	491:499	arg1	immunity					504:511	immunity	504:511	immunity	504:511	Both the degree of methyl-esterification (DM) and the distribution of methyl-esters (degree of blockiness; DB) of pectins contribute to this influence on immunity, but whether and how the DB impacts immunity through microbiota-dependent effects in the large intestine is unknown.
34369649	4	26	theme	low	890:892	arg1	pectins					906:912	Both low and high DB pectins	885:912	METHODS AND RESULTS Both low and high DB pectins	865:912	METHODS AND RESULTS Both low and high DB pectins induce a more rich and diverse microbiota composition.
34369649	1	27	theme	Toll-like	228:236	arg1	TLRs					249:252	TLRs	249:252	TLRs	249:252	INTRODUCTION Pectins have anti-inflammatory properties on intestinal immunity through direct interactions on Toll-like receptors (TLRs) in the small intestine or via stimulating microbiota-dependent effects in the large intestine.
34369649	1	27	theme	Toll-like	228:236	arg1	receptors					238:246	Toll-like receptors	228:246	Toll-like receptors (TLRs) in the small intestine	228:276	INTRODUCTION Pectins have anti-inflammatory properties on intestinal immunity through direct interactions on Toll-like receptors (TLRs) in the small intestine or via stimulating microbiota-dependent effects in the large intestine.
34369649	2	28	theme	pectins	464:470	arg1	degree					435:440	degree	435:440	degree of blockiness	435:454	Both the degree of methyl-esterification (DM) and the distribution of methyl-esters (degree of blockiness; DB) of pectins contribute to this influence on immunity, but whether and how the DB impacts immunity through microbiota-dependent effects in the large intestine is unknown.
34369649	2	28	theme	pectins	464:470	arg1	methyl-esters					420:432	methyl-esters	420:432	methyl-esters (degree of blockiness; DB) of pectins	420:470	Both the degree of methyl-esterification (DM) and the distribution of methyl-esters (degree of blockiness; DB) of pectins contribute to this influence on immunity, but whether and how the DB impacts immunity through microbiota-dependent effects in the large intestine is unknown.
34369649	6	29	theme	tissue	1326:1331	arg1	inflammation					1333:1344	tissue inflammation	1326:1344	tissue inflammation	1326:1344	Through these effects, both low and high DB pectins attenuate C. rodentium induced colitis resulting in reduced intestinal damage, reduced numbers of Th1-cells, which are increased in case of C. rodentium induced colitis, and reduced levels of GATA3+ Tregs, which are related to tissue inflammation.
34369649	3	30	from	impact	773:778	arg1	composition					809:819	intestinal microbiota composition	787:819	intestinal microbiota composition	787:819	Therefore, this study tests pectins that structurally differ in DB in a mouse model with Citrobacter rodentium induced colitis and studies the impact on the intestinal microbiota composition and associated attenuation of inflammation.
34369649	3	30	from	impact	773:778	arg1	attenuation					836:846	associated attenuation	825:846	associated attenuation	825:846	Therefore, this study tests pectins that structurally differ in DB in a mouse model with Citrobacter rodentium induced colitis and studies the impact on the intestinal microbiota composition and associated attenuation of inflammation.
34369649	6	31	theme	low	1075:1077	arg1	pectins					1091:1097	both low and high DB pectins	1070:1097	both low and high DB pectins	1070:1097	Through these effects, both low and high DB pectins attenuate C. rodentium induced colitis resulting in reduced intestinal damage, reduced numbers of Th1-cells, which are increased in case of C. rodentium induced colitis, and reduced levels of GATA3+ Tregs, which are related to tissue inflammation.
34369649	7	32	theme	colonic	1395:1401	arg1	inflammation					1403:1414	C. rodentium induced colonic inflammation	1374:1414	C. rodentium induced colonic inflammation	1374:1414	CONCLUSION Pectins prevent C. rodentium induced colonic inflammation by lowering the C. rodentium load in the caecum independently of the DB.
34369649	5	33	theme	rodentium	1019:1027	arg1	load					1008:1011	the bacterial load	994:1011	the bacterial load of C. rodentium in cecal digesta	994:1044	These pectins also lower the bacterial load of C. rodentium in cecal digesta.
34369649	2	34	theme	microbiota-dependent	566:585	arg1	effects					587:593	microbiota-dependent effects	566:593	microbiota-dependent effects in the large intestine	566:616	Both the degree of methyl-esterification (DM) and the distribution of methyl-esters (degree of blockiness; DB) of pectins contribute to this influence on immunity, but whether and how the DB impacts immunity through microbiota-dependent effects in the large intestine is unknown.
34369649	1	35	theme	INTRODUCTION	119:130	arg1	Pectins					132:138	INTRODUCTION Pectins	119:138	INTRODUCTION Pectins	119:138	INTRODUCTION Pectins have anti-inflammatory properties on intestinal immunity through direct interactions on Toll-like receptors (TLRs) in the small intestine or via stimulating microbiota-dependent effects in the large intestine.
34369649	5	36	theme	cecal	1032:1036	arg1	digesta					1038:1044	cecal digesta	1032:1044	cecal digesta	1032:1044	These pectins also lower the bacterial load of C. rodentium in cecal digesta.
34369649	4	37	dep	METHODS	865:871	arg1	pectins					906:912	Both low and high DB pectins	885:912	METHODS AND RESULTS Both low and high DB pectins	865:912	METHODS AND RESULTS Both low and high DB pectins induce a more rich and diverse microbiota composition.
34369649	1	38	theme	large	333:337	arg1	intestine					339:347	the large intestine	329:347	the large intestine	329:347	INTRODUCTION Pectins have anti-inflammatory properties on intestinal immunity through direct interactions on Toll-like receptors (TLRs) in the small intestine or via stimulating microbiota-dependent effects in the large intestine.
34369649	1	39	from	effects	318:324	arg1	intestine					339:347	the large intestine	329:347	the large intestine	329:347	INTRODUCTION Pectins have anti-inflammatory properties on intestinal immunity through direct interactions on Toll-like receptors (TLRs) in the small intestine or via stimulating microbiota-dependent effects in the large intestine.
34369649	3	40	dep	composition	809:819	arg1	the					783:785	the	783:785	the	783:785	Therefore, this study tests pectins that structurally differ in DB in a mouse model with Citrobacter rodentium induced colitis and studies the impact on the intestinal microbiota composition and associated attenuation of inflammation.
34369649	6	41	theme	reduced	1151:1157	arg1	damage					1170:1175	reduced intestinal damage	1151:1175	reduced intestinal damage	1151:1175	Through these effects, both low and high DB pectins attenuate C. rodentium induced colitis resulting in reduced intestinal damage, reduced numbers of Th1-cells, which are increased in case of C. rodentium induced colitis, and reduced levels of GATA3+ Tregs, which are related to tissue inflammation.
34369649	5	42	dep	load	1008:1011	arg1	lower					988:992	lower	988:992	lower	988:992	These pectins also lower the bacterial load of C. rodentium in cecal digesta.
34369649	4	43	theme	microbiota	945:954	arg1	composition					956:966	a more rich and diverse microbiota composition	921:966	a more rich and diverse microbiota composition	921:966	METHODS AND RESULTS Both low and high DB pectins induce a more rich and diverse microbiota composition.
34369649	2	44	theme	blockiness	445:454	arg1	degree					435:440	degree	435:440	degree of blockiness	435:454	Both the degree of methyl-esterification (DM) and the distribution of methyl-esters (degree of blockiness; DB) of pectins contribute to this influence on immunity, but whether and how the DB impacts immunity through microbiota-dependent effects in the large intestine is unknown.
34369649	2	44	theme	blockiness	445:454	arg1	methyl-esters					420:432	methyl-esters	420:432	methyl-esters (degree of blockiness; DB) of pectins	420:470	Both the degree of methyl-esterification (DM) and the distribution of methyl-esters (degree of blockiness; DB) of pectins contribute to this influence on immunity, but whether and how the DB impacts immunity through microbiota-dependent effects in the large intestine is unknown.
34369649	3	45	theme	intestinal	787:796	arg1	composition					809:819	intestinal microbiota composition	787:819	intestinal microbiota composition	787:819	Therefore, this study tests pectins that structurally differ in DB in a mouse model with Citrobacter rodentium induced colitis and studies the impact on the intestinal microbiota composition and associated attenuation of inflammation.
34369649	2	46	from	effects	587:593	arg1	intestine					608:616	the large intestine	598:616	the large intestine	598:616	Both the degree of methyl-esterification (DM) and the distribution of methyl-esters (degree of blockiness; DB) of pectins contribute to this influence on immunity, but whether and how the DB impacts immunity through microbiota-dependent effects in the large intestine is unknown.
34369649	7	47	theme	induced	1387:1393	arg1	inflammation					1403:1414	C. rodentium induced colonic inflammation	1374:1414	C. rodentium induced colonic inflammation	1374:1414	CONCLUSION Pectins prevent C. rodentium induced colonic inflammation by lowering the C. rodentium load in the caecum independently of the DB.
34369649	3	48	theme	microbiota	798:807	arg1	composition					809:819	intestinal microbiota composition	787:819	intestinal microbiota composition	787:819	Therefore, this study tests pectins that structurally differ in DB in a mouse model with Citrobacter rodentium induced colitis and studies the impact on the intestinal microbiota composition and associated attenuation of inflammation.
34369649	2	49	theme	large	602:606	arg1	intestine					608:616	the large intestine	598:616	the large intestine	598:616	Both the degree of methyl-esterification (DM) and the distribution of methyl-esters (degree of blockiness; DB) of pectins contribute to this influence on immunity, but whether and how the DB impacts immunity through microbiota-dependent effects in the large intestine is unknown.
34369649	4	50	theme	rich	928:931	arg1	composition					956:966	a more rich and diverse microbiota composition	921:966	a more rich and diverse microbiota composition	921:966	METHODS AND RESULTS Both low and high DB pectins induce a more rich and diverse microbiota composition.
34369649	3	51	theme	mouse	702:706	arg1	model					708:712	a mouse model	700:712	a mouse model with Citrobacter rodentium induced colitis and studies the impact on the intestinal microbiota composition and associated attenuation of inflammation	700:862	Therefore, this study tests pectins that structurally differ in DB in a mouse model with Citrobacter rodentium induced colitis and studies the impact on the intestinal microbiota composition and associated attenuation of inflammation.
34369649	4	52	theme	diverse	937:943	arg1	composition					956:966	a more rich and diverse microbiota composition	921:966	a more rich and diverse microbiota composition	921:966	METHODS AND RESULTS Both low and high DB pectins induce a more rich and diverse microbiota composition.
34369649	7	53	theme	C.	1374:1375	arg1	rodentium					1377:1385	C. rodentium	1374:1385	C. rodentium induced colonic inflammation	1374:1414	CONCLUSION Pectins prevent C. rodentium induced colonic inflammation by lowering the C. rodentium load in the caecum independently of the DB.
34369649	1	54	theme	anti-inflammatory	145:161	arg1	properties					163:172	anti-inflammatory properties	145:172	anti-inflammatory properties	145:172	INTRODUCTION Pectins have anti-inflammatory properties on intestinal immunity through direct interactions on Toll-like receptors (TLRs) in the small intestine or via stimulating microbiota-dependent effects in the large intestine.
34369649	7	55	theme	rodentium	1435:1443	arg1	load					1445:1448	the C. rodentium load	1428:1448	the C. rodentium load in the caecum independently of the DB	1428:1486	CONCLUSION Pectins prevent C. rodentium induced colonic inflammation by lowering the C. rodentium load in the caecum independently of the DB.
34369649	2	56	dep	whether	518:524	arg1	impacts					541:547	impacts	541:547	impacts immunity through microbiota-dependent effects in the large intestine	541:616	Both the degree of methyl-esterification (DM) and the distribution of methyl-esters (degree of blockiness; DB) of pectins contribute to this influence on immunity, but whether and how the DB impacts immunity through microbiota-dependent effects in the large intestine is unknown.
34369649	7	57	theme	CONCLUSION	1347:1356	arg1	Pectins					1358:1364	CONCLUSION Pectins	1347:1364	CONCLUSION Pectins	1347:1364	CONCLUSION Pectins prevent C. rodentium induced colonic inflammation by lowering the C. rodentium load in the caecum independently of the DB.
34369649	2	58	theme	methyl-esterification	369:389	arg1	distribution					404:415	the distribution	400:415	the distribution of methyl-esters (degree of blockiness; DB) of pectins	400:470	Both the degree of methyl-esterification (DM) and the distribution of methyl-esters (degree of blockiness; DB) of pectins contribute to this influence on immunity, but whether and how the DB impacts immunity through microbiota-dependent effects in the large intestine is unknown.
34369649	2	58	theme	methyl-esterification	369:389	arg1	degree					359:364	the degree	355:364	the degree of methyl-esterification (DM)	355:394	Both the degree of methyl-esterification (DM) and the distribution of methyl-esters (degree of blockiness; DB) of pectins contribute to this influence on immunity, but whether and how the DB impacts immunity through microbiota-dependent effects in the large intestine is unknown.
34369649	6	59	theme	intestinal	1159:1168	arg1	damage					1170:1175	reduced intestinal damage	1151:1175	reduced intestinal damage	1151:1175	Through these effects, both low and high DB pectins attenuate C. rodentium induced colitis resulting in reduced intestinal damage, reduced numbers of Th1-cells, which are increased in case of C. rodentium induced colitis, and reduced levels of GATA3+ Tregs, which are related to tissue inflammation.
34369649	3	60	theme	associated	825:834	arg1	attenuation					836:846	associated attenuation	825:846	associated attenuation	825:846	Therefore, this study tests pectins that structurally differ in DB in a mouse model with Citrobacter rodentium induced colitis and studies the impact on the intestinal microbiota composition and associated attenuation of inflammation.
34369649	6	61	theme	GATA3+	1291:1296	arg1	Tregs					1298:1302	GATA3+ Tregs	1291:1302	GATA3+ Tregs	1291:1302	Through these effects, both low and high DB pectins attenuate C. rodentium induced colitis resulting in reduced intestinal damage, reduced numbers of Th1-cells, which are increased in case of C. rodentium induced colitis, and reduced levels of GATA3+ Tregs, which are related to tissue inflammation.
34369649	5	62	theme	C.	1016:1017	arg1	rodentium					1019:1027	C. rodentium	1016:1027	C. rodentium	1016:1027	These pectins also lower the bacterial load of C. rodentium in cecal digesta.
34369649	1	63	theme	small	262:266	arg1	intestine					268:276	the small intestine	258:276	the small intestine	258:276	INTRODUCTION Pectins have anti-inflammatory properties on intestinal immunity through direct interactions on Toll-like receptors (TLRs) in the small intestine or via stimulating microbiota-dependent effects in the large intestine.
34369649	2	64	dep	degree	435:440	arg1	DB					457:458	DB	457:458	DB	457:458	Both the degree of methyl-esterification (DM) and the distribution of methyl-esters (degree of blockiness; DB) of pectins contribute to this influence on immunity, but whether and how the DB impacts immunity through microbiota-dependent effects in the large intestine is unknown.
34369649	1	65	from	receptors	238:246	arg1	intestine					268:276	the small intestine	258:276	the small intestine	258:276	INTRODUCTION Pectins have anti-inflammatory properties on intestinal immunity through direct interactions on Toll-like receptors (TLRs) in the small intestine or via stimulating microbiota-dependent effects in the large intestine.
34369649	1	66	theme	intestinal	177:186	arg1	immunity					188:195	intestinal immunity	177:195	intestinal immunity	177:195	INTRODUCTION Pectins have anti-inflammatory properties on intestinal immunity through direct interactions on Toll-like receptors (TLRs) in the small intestine or via stimulating microbiota-dependent effects in the large intestine.
34369649	0	67	theme	Methyl-Esters	56:68	arg1	Distribution					40:51	the Distribution	36:51	the Distribution of Methyl-Esters	36:68	Pectins that Structurally Differ in the Distribution of Methyl-Esters Attenuate Citrobacter rodentium-Induced Colitis.
34369649	7	68	theme	DB	1485:1486	arg1	caecum					1457:1462	the caecum	1453:1462	the caecum independently of the DB	1453:1486	CONCLUSION Pectins prevent C. rodentium induced colonic inflammation by lowering the C. rodentium load in the caecum independently of the DB.
34369649	6	69	theme	rodentium	1242:1250	arg1	colitis					1260:1266	C. rodentium induced colitis	1239:1266	C. rodentium induced colitis	1239:1266	Through these effects, both low and high DB pectins attenuate C. rodentium induced colitis resulting in reduced intestinal damage, reduced numbers of Th1-cells, which are increased in case of C. rodentium induced colitis, and reduced levels of GATA3+ Tregs, which are related to tissue inflammation.
34369649	1	70	from	interactions	212:223	arg1	TLRs					249:252	TLRs	249:252	TLRs	249:252	INTRODUCTION Pectins have anti-inflammatory properties on intestinal immunity through direct interactions on Toll-like receptors (TLRs) in the small intestine or via stimulating microbiota-dependent effects in the large intestine.
34369649	1	70	from	interactions	212:223	arg1	receptors					238:246	Toll-like receptors	228:246	Toll-like receptors (TLRs) in the small intestine	228:276	INTRODUCTION Pectins have anti-inflammatory properties on intestinal immunity through direct interactions on Toll-like receptors (TLRs) in the small intestine or via stimulating microbiota-dependent effects in the large intestine.
34369649	6	71	theme	C.	1109:1110	arg1	rodentium					1112:1120	C. rodentium	1109:1120	C. rodentium induced colitis resulting in reduced intestinal damage, reduced numbers of Th1-cells, which are increased in case of C. rodentium induced colitis, and reduced levels of GATA3+ Tregs, which are related to tissue inflammation	1109:1344	Through these effects, both low and high DB pectins attenuate C. rodentium induced colitis resulting in reduced intestinal damage, reduced numbers of Th1-cells, which are increased in case of C. rodentium induced colitis, and reduced levels of GATA3+ Tregs, which are related to tissue inflammation.
32858903	4	0	theme	specific	792:799	arg1	method					801:806	a specific method	790:806	a specific method that simultaneously assessed the level of taste preferences and the sensitivity threshold for a given taste	790:914	The evaluation of sweet taste perception was carried out using a specific method that simultaneously assessed the level of taste preferences and the sensitivity threshold for a given taste.
32858903	8	1	theme	correlation	1509:1519	arg1	existence					1478:1486	the existence	1474:1486	the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries	1474:1825	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	3	2	theme	teeth	635:639	arg1	dmft					648:651	dmft	648:651	dmft	648:651	The study consisted of collecting questionnaire data and assessment of dental status using the decayed, missing, filled in primary teeth index (dmft) and the International Caries Detection and Assessment System (ICDAS II).
32858903	3	2	theme	teeth	635:639	arg1	index					641:645	primary teeth index (dmft) and the International	627:674	index	641:645	The study consisted of collecting questionnaire data and assessment of dental status using the decayed, missing, filled in primary teeth index (dmft) and the International Caries Detection and Assessment System (ICDAS II).
32858903	1	3	theme	sweets	378:383	arg1	consumption					385:395	sweets consumption	378:395	sweets consumption in children	378:407	The aim of the study was to verify the hypothesis about differences in sweet taste perception in the group of preschool children with and without caries, and to determine its relationship with cariogenic microbiota and the frequency of sweets consumption in children.
32858903	7	4	theme	similar	1327:1333	arg1	relationship					1335:1346	A similar relationship	1325:1346	A similar relationship	1325:1346	A similar relationship was also observed for frequent snacking between meals (p = 0.0038, chi-square test).
32858903	9	5	theme	mutans	1942:1947	arg1	presence					1949:1956	S. mutans presence	1939:1956	S. mutans presence	1939:1956	Additionally, children with an elevated sucrose taste threshold were more than 10-times more likely to develop S. mutans presence (OR = 10.21; 95% CI 3.11-33.44).
32858903	6	6	theme	solution	1201:1208	arg1	concentration					1210:1222	the preferred sucrose solution concentration	1179:1222	the preferred sucrose solution concentration was >4 g/L	1179:1233	The sweet taste perception of children with caries was characterized by a lower susceptibility to sucrose (the preferred sucrose solution concentration was >4 g/L) compared to children without caries (in the range ≤ 4 g/L, p = 0.0015, chi-square test).
32858903	8	7	theme	cariogenic	1599:1608	arg1	process					1610:1616	the cariogenic process	1595:1616	the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II)	1595:1665	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	9	8	dep	develop	1931:1937	arg1	CI					1975:1976	= 10.21; 95% CI 3.11-33.44	1962:1987	= 10.21; 95% CI 3.11-33.44	1962:1987	Additionally, children with an elevated sucrose taste threshold were more than 10-times more likely to develop S. mutans presence (OR = 10.21; 95% CI 3.11-33.44).
32858903	10	9	theme	taste	2043:2047	arg1	preferences					2049:2059	taste preferences	2043:2059	taste preferences	2043:2059	The results of this study suggest the future use of taste preferences in children as a diagnostic tool for the early detection of increased susceptibility to caries through microbial dysbiosis towards specific species of microorganisms.
32858903	6	10	theme	children	1102:1109	arg1	perception					1088:1097	The sweet taste perception	1072:1097	The sweet taste perception of children with caries	1072:1121	The sweet taste perception of children with caries was characterized by a lower susceptibility to sucrose (the preferred sucrose solution concentration was >4 g/L) compared to children without caries (in the range ≤ 4 g/L, p = 0.0015, chi-square test).
32858903	8	11	theme	repeated	1741:1748	arg1	consumption					1750:1760	frequent and repeated consumption	1728:1760	frequent and repeated consumption of sweets during the day	1728:1785	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	9	12	with	children	1842:1849	arg1	threshold					1882:1890	an elevated sucrose taste threshold	1856:1890	an elevated sucrose taste threshold	1856:1890	Additionally, children with an elevated sucrose taste threshold were more than 10-times more likely to develop S. mutans presence (OR = 10.21; 95% CI 3.11-33.44).
32858903	8	13	theme	p	1714:1714	arg1	sweets					1706:1711	consuming sweets	1696:1711	consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries	1696:1825	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	8	13	theme	p	1714:1714	arg1	≤					1716:1716	p ≤ 0.001	1714:1722	p ≤ 0.001 for frequent and repeated consumption of sweets during the day	1714:1785	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	4	14	theme	taste	850:854	arg1	preferences					856:866	taste preferences	850:866	taste preferences	850:866	The evaluation of sweet taste perception was carried out using a specific method that simultaneously assessed the level of taste preferences and the sensitivity threshold for a given taste.
32858903	3	15	theme	Caries	676:681	arg1	Detection					683:691	the decayed, missing, filled in primary teeth index (dmft) and the International Caries Detection and Assessment System	595:713	Detection	683:691	The study consisted of collecting questionnaire data and assessment of dental status using the decayed, missing, filled in primary teeth index (dmft) and the International Caries Detection and Assessment System (ICDAS II).
32858903	3	15	theme	Caries	676:681	arg1	II					722:723	ICDAS II	716:723	ICDAS II	716:723	The study consisted of collecting questionnaire data and assessment of dental status using the decayed, missing, filled in primary teeth index (dmft) and the International Caries Detection and Assessment System (ICDAS II).
32858903	5	16	theme	compositions	1010:1021	arg1	assessment					963:972	the assessment	959:972	the assessment of the quantitative and qualitative compositions of the oral microbiota of the examined children	959:1069	The microbiological analysis consisted of the assessment of the quantitative and qualitative compositions of the oral microbiota of the examined children.
32858903	3	17	theme	ICDAS	716:720	arg1	Detection					683:691	the decayed, missing, filled in primary teeth index (dmft) and the International Caries Detection and Assessment System	595:713	Detection	683:691	The study consisted of collecting questionnaire data and assessment of dental status using the decayed, missing, filled in primary teeth index (dmft) and the International Caries Detection and Assessment System (ICDAS II).
32858903	3	17	theme	ICDAS	716:720	arg1	II					722:723	ICDAS II	716:723	ICDAS II	716:723	The study consisted of collecting questionnaire data and assessment of dental status using the decayed, missing, filled in primary teeth index (dmft) and the International Caries Detection and Assessment System (ICDAS II).
32858903	8	18	theme	frequent	1728:1735	arg1	consumption					1750:1760	frequent and repeated consumption	1728:1760	frequent and repeated consumption of sweets during the day	1728:1785	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	10	19	theme	early	2102:2106	arg1	detection					2108:2116	the early detection	2098:2116	the early detection of increased susceptibility to caries through microbial dysbiosis towards specific species of microorganisms	2098:2225	The results of this study suggest the future use of taste preferences in children as a diagnostic tool for the early detection of increased susceptibility to caries through microbial dysbiosis towards specific species of microorganisms.
32858903	1	20	from	perception	225:234	arg1	group					243:247	the group	239:247	the group of preschool children with and without caries	239:293	The aim of the study was to verify the hypothesis about differences in sweet taste perception in the group of preschool children with and without caries, and to determine its relationship with cariogenic microbiota and the frequency of sweets consumption in children.
32858903	1	20	from	perception	225:234	arg1	children					262:269	preschool children	252:269	preschool children with and without caries	252:293	The aim of the study was to verify the hypothesis about differences in sweet taste perception in the group of preschool children with and without caries, and to determine its relationship with cariogenic microbiota and the frequency of sweets consumption in children.
32858903	1	21	theme	consumption	385:395	arg1	microbiota					346:355	cariogenic microbiota	335:355	cariogenic microbiota	335:355	The aim of the study was to verify the hypothesis about differences in sweet taste perception in the group of preschool children with and without caries, and to determine its relationship with cariogenic microbiota and the frequency of sweets consumption in children.
32858903	1	21	theme	consumption	385:395	arg1	frequency					365:373	the frequency	361:373	the frequency of sweets consumption in children	361:407	The aim of the study was to verify the hypothesis about differences in sweet taste perception in the group of preschool children with and without caries, and to determine its relationship with cariogenic microbiota and the frequency of sweets consumption in children.
32858903	4	22	theme	given	904:908	arg1	taste					910:914	a given taste	902:914	a given taste	902:914	The evaluation of sweet taste perception was carried out using a specific method that simultaneously assessed the level of taste preferences and the sensitivity threshold for a given taste.
32858903	6	23	dep	sucrose	1170:1176	arg1	concentration					1210:1222	the preferred sucrose solution concentration	1179:1222	the preferred sucrose solution concentration was >4 g/L	1179:1233	The sweet taste perception of children with caries was characterized by a lower susceptibility to sucrose (the preferred sucrose solution concentration was >4 g/L) compared to children without caries (in the range ≤ 4 g/L, p = 0.0015, chi-square test).
32858903	0	24	with	Children	120:127	arg1	Caries					134:139	Caries	134:139	Caries	134:139	Differences in Sweet Taste Perception and Its Association with the Streptococcus mutans Cariogenic Profile in Preschool Children with Caries.
32858903	6	25	theme	=	1297:1297	arg1	caries					1265:1270	caries	1265:1270	caries (in the range ≤ 4 g/L, p = 0.0015, chi-square test)	1265:1322	The sweet taste perception of children with caries was characterized by a lower susceptibility to sucrose (the preferred sucrose solution concentration was >4 g/L) compared to children without caries (in the range ≤ 4 g/L, p = 0.0015, chi-square test).
32858903	6	25	theme	=	1297:1297	arg1	0.0015					1299:1304	p = 0.0015	1295:1304	p = 0.0015	1295:1304	The sweet taste perception of children with caries was characterized by a lower susceptibility to sucrose (the preferred sucrose solution concentration was >4 g/L) compared to children without caries (in the range ≤ 4 g/L, p = 0.0015, chi-square test).
32858903	5	26	theme	children	1062:1069	arg1	microbiota					1035:1044	the oral microbiota	1026:1044	the oral microbiota of the examined children	1026:1069	The microbiological analysis consisted of the assessment of the quantitative and qualitative compositions of the oral microbiota of the examined children.
32858903	3	27	theme	dental	575:580	arg1	status					582:587	dental status	575:587	dental status	575:587	The study consisted of collecting questionnaire data and assessment of dental status using the decayed, missing, filled in primary teeth index (dmft) and the International Caries Detection and Assessment System (ICDAS II).
32858903	10	28	from	use	2036:2038	arg1	children					2064:2071	children	2064:2071	children	2064:2071	The results of this study suggest the future use of taste preferences in children as a diagnostic tool for the early detection of increased susceptibility to caries through microbial dysbiosis towards specific species of microorganisms.
32858903	0	29	theme	Streptococcus	67:79	arg1	Profile					99:105	the Streptococcus mutans Cariogenic Profile	63:105	the Streptococcus mutans Cariogenic Profile in Preschool Children with Caries	63:139	Differences in Sweet Taste Perception and Its Association with the Streptococcus mutans Cariogenic Profile in Preschool Children with Caries.
32858903	6	30	from	0.0015	1299:1304	arg1	g/L					1290:1292	the range ≤ 4 g/L	1276:1292	the range ≤ 4 g/L	1276:1292	The sweet taste perception of children with caries was characterized by a lower susceptibility to sucrose (the preferred sucrose solution concentration was >4 g/L) compared to children without caries (in the range ≤ 4 g/L, p = 0.0015, chi-square test).
32858903	4	31	theme	sensitivity	876:886	arg1	threshold					888:896	the sensitivity threshold	872:896	the sensitivity threshold for a given taste	872:914	The evaluation of sweet taste perception was carried out using a specific method that simultaneously assessed the level of taste preferences and the sensitivity threshold for a given taste.
32858903	0	32	from	Differences	0:10	arg1	Perception					27:36	Sweet Taste Perception	15:36	Sweet Taste Perception	15:36	Differences in Sweet Taste Perception and Its Association with the Streptococcus mutans Cariogenic Profile in Preschool Children with Caries.
32858903	0	32	from	Differences	0:10	arg1	Association					46:56	Its Association	42:56	Its Association with the Streptococcus mutans Cariogenic Profile in Preschool Children with Caries	42:139	Differences in Sweet Taste Perception and Its Association with the Streptococcus mutans Cariogenic Profile in Preschool Children with Caries.
32858903	0	33	theme	Cariogenic	88:97	arg1	Profile					99:105	the Streptococcus mutans Cariogenic Profile	63:105	the Streptococcus mutans Cariogenic Profile in Preschool Children with Caries	63:139	Differences in Sweet Taste Perception and Its Association with the Streptococcus mutans Cariogenic Profile in Preschool Children with Caries.
32858903	5	34	theme	microbiota	1035:1044	arg1	compositions					1010:1021	the quantitative and qualitative compositions	977:1021	the quantitative and qualitative compositions of the oral microbiota of the examined children	977:1069	The microbiological analysis consisted of the assessment of the quantitative and qualitative compositions of the oral microbiota of the examined children.
32858903	8	35	dep	process	1610:1616	arg1	p					1643:1643	p = 0.012	1643:1651	p = 0.012 for ICDAS II	1643:1664	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	8	35	dep	process	1610:1616	arg1	p					1619:1619	p	1619:1619	p = 0.007 for dmft	1619:1636	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	1	36	from	frequency	365:373	arg1	children					400:407	children	400:407	children	400:407	The aim of the study was to verify the hypothesis about differences in sweet taste perception in the group of preschool children with and without caries, and to determine its relationship with cariogenic microbiota and the frequency of sweets consumption in children.
32858903	8	37	dep	≤	1716:1716	arg1	test					1797:1800	Spearman test	1788:1800	Spearman test	1788:1800	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	1	38	theme	preschool	252:260	arg1	children					262:269	preschool children	252:269	preschool children with and without caries	252:293	The aim of the study was to verify the hypothesis about differences in sweet taste perception in the group of preschool children with and without caries, and to determine its relationship with cariogenic microbiota and the frequency of sweets consumption in children.
32858903	0	39	dep	Streptococcus	67:79	arg1	mutans					81:86	mutans	81:86	mutans	81:86	Differences in Sweet Taste Perception and Its Association with the Streptococcus mutans Cariogenic Profile in Preschool Children with Caries.
32858903	2	40	theme	study	414:418	arg1	group					420:424	The study group	410:424	The study group	410:424	The study group included of 63 children aged 2-6 years: 32 with caries and 31 without caries.
32858903	0	41	theme	Sweet	15:19	arg1	Perception					27:36	Sweet Taste Perception	15:36	Sweet Taste Perception	15:36	Differences in Sweet Taste Perception and Its Association with the Streptococcus mutans Cariogenic Profile in Preschool Children with Caries.
32858903	2	42	theme	aged	450:453	arg1	children					441:448	63 children	438:448	63 children aged 2-6 years	438:463	The study group included of 63 children aged 2-6 years: 32 with caries and 31 without caries.
32858903	9	43	theme	elevated	1859:1866	arg1	threshold					1882:1890	an elevated sucrose taste threshold	1856:1890	an elevated sucrose taste threshold	1856:1890	Additionally, children with an elevated sucrose taste threshold were more than 10-times more likely to develop S. mutans presence (OR = 10.21; 95% CI 3.11-33.44).
32858903	4	44	theme	sweet	745:749	arg1	perception					757:766	sweet taste perception	745:766	sweet taste perception	745:766	The evaluation of sweet taste perception was carried out using a specific method that simultaneously assessed the level of taste preferences and the sensitivity threshold for a given taste.
32858903	10	45	theme	microorganisms	2212:2225	arg1	species					2201:2207	specific species	2192:2207	specific species of microorganisms	2192:2225	The results of this study suggest the future use of taste preferences in children as a diagnostic tool for the early detection of increased susceptibility to caries through microbial dysbiosis towards specific species of microorganisms.
32858903	8	46	theme	studied	1449:1455	arg1	variables					1457:1465	studied variables	1449:1465	studied variables	1449:1465	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	3	47	theme	decayed	599:605	arg1	Detection					683:691	the decayed, missing, filled in primary teeth index (dmft) and the International Caries Detection and Assessment System	595:713	Detection	683:691	The study consisted of collecting questionnaire data and assessment of dental status using the decayed, missing, filled in primary teeth index (dmft) and the International Caries Detection and Assessment System (ICDAS II).
32858903	3	47	theme	decayed	599:605	arg1	II					722:723	ICDAS II	716:723	ICDAS II	716:723	The study consisted of collecting questionnaire data and assessment of dental status using the decayed, missing, filled in primary teeth index (dmft) and the International Caries Detection and Assessment System (ICDAS II).
32858903	4	48	theme	perception	757:766	arg1	evaluation					731:740	The evaluation	727:740	The evaluation of sweet taste perception	727:766	The evaluation of sweet taste perception was carried out using a specific method that simultaneously assessed the level of taste preferences and the sensitivity threshold for a given taste.
32858903	8	49	theme	sweets	1706:1711	arg1	frequency					1683:1691	the frequency	1679:1691	the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries	1679:1825	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	8	49	theme	sweets	1706:1711	arg1	process					1610:1616	the cariogenic process	1595:1616	the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II)	1595:1665	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	8	50	from	sweets	1706:1711	arg1	children					1806:1813	children	1806:1813	children with caries	1806:1825	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	3	51	theme	primary	627:633	arg1	dmft					648:651	dmft	648:651	dmft	648:651	The study consisted of collecting questionnaire data and assessment of dental status using the decayed, missing, filled in primary teeth index (dmft) and the International Caries Detection and Assessment System (ICDAS II).
32858903	3	51	theme	primary	627:633	arg1	index					641:645	primary teeth index (dmft) and the International	627:674	index	641:645	The study consisted of collecting questionnaire data and assessment of dental status using the decayed, missing, filled in primary teeth index (dmft) and the International Caries Detection and Assessment System (ICDAS II).
32858903	8	52	with	children	1806:1813	arg1	caries					1820:1825	caries	1820:1825	caries	1820:1825	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	5	53	theme	microbiological	921:935	arg1	analysis					937:944	The microbiological analysis	917:944	The microbiological analysis	917:944	The microbiological analysis consisted of the assessment of the quantitative and qualitative compositions of the oral microbiota of the examined children.
32858903	8	54	theme	sweet	1547:1551	arg1	taste					1553:1557	sweet taste	1547:1557	sweet taste	1547:1557	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	1	55	theme	taste	219:223	arg1	perception					225:234	sweet taste perception	213:234	sweet taste perception in the group of preschool children with and without caries	213:293	The aim of the study was to verify the hypothesis about differences in sweet taste perception in the group of preschool children with and without caries, and to determine its relationship with cariogenic microbiota and the frequency of sweets consumption in children.
32858903	9	56	dep	=	1962:1962	arg1	%					1973:1973	10.21; 95%	1964:1973	10.21; 95%	1964:1973	Additionally, children with an elevated sucrose taste threshold were more than 10-times more likely to develop S. mutans presence (OR = 10.21; 95% CI 3.11-33.44).
32858903	6	57	theme	p	1295:1295	arg1	caries					1265:1270	caries	1265:1270	caries (in the range ≤ 4 g/L, p = 0.0015, chi-square test)	1265:1322	The sweet taste perception of children with caries was characterized by a lower susceptibility to sucrose (the preferred sucrose solution concentration was >4 g/L) compared to children without caries (in the range ≤ 4 g/L, p = 0.0015, chi-square test).
32858903	6	57	theme	p	1295:1295	arg1	0.0015					1299:1304	p = 0.0015	1295:1304	p = 0.0015	1295:1304	The sweet taste perception of children with caries was characterized by a lower susceptibility to sucrose (the preferred sucrose solution concentration was >4 g/L) compared to children without caries (in the range ≤ 4 g/L, p = 0.0015, chi-square test).
32858903	8	58	theme	frequency	1683:1691	arg1	correlation					1509:1519	a strong positive correlation	1491:1519	a strong positive correlation between the perception of sweet taste and the occurrence	1491:1576	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	8	58	theme	frequency	1683:1691	arg1	intensity					1582:1590	intensity	1582:1590	intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries	1582:1825	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	0	59	with	Perception	27:36	arg1	Profile					99:105	the Streptococcus mutans Cariogenic Profile	63:105	the Streptococcus mutans Cariogenic Profile in Preschool Children with Caries	63:139	Differences in Sweet Taste Perception and Its Association with the Streptococcus mutans Cariogenic Profile in Preschool Children with Caries.
32858903	7	60	theme	frequent	1370:1377	arg1	snacking					1379:1386	frequent snacking	1370:1386	frequent snacking between meals (p = 0.0038, chi-square test)	1370:1430	A similar relationship was also observed for frequent snacking between meals (p = 0.0038, chi-square test).
32858903	10	61	theme	future	2029:2034	arg1	use					2036:2038	the future use	2025:2038	the future use of taste preferences in children	2025:2071	The results of this study suggest the future use of taste preferences in children as a diagnostic tool for the early detection of increased susceptibility to caries through microbial dysbiosis towards specific species of microorganisms.
32858903	10	61	theme	future	2029:2034	arg1	tool					2089:2092	a diagnostic tool	2076:2092	a diagnostic tool for the early detection of increased susceptibility to caries through microbial dysbiosis towards specific species of microorganisms	2076:2225	The results of this study suggest the future use of taste preferences in children as a diagnostic tool for the early detection of increased susceptibility to caries through microbial dysbiosis towards specific species of microorganisms.
32858903	9	62	theme	taste	1876:1880	arg1	threshold					1882:1890	an elevated sucrose taste threshold	1856:1890	an elevated sucrose taste threshold	1856:1890	Additionally, children with an elevated sucrose taste threshold were more than 10-times more likely to develop S. mutans presence (OR = 10.21; 95% CI 3.11-33.44).
32858903	8	63	theme	positive	1500:1507	arg1	correlation					1509:1519	a strong positive correlation	1491:1519	a strong positive correlation between the perception of sweet taste and the occurrence	1491:1576	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	8	64	theme	consuming	1696:1704	arg1	sweets					1706:1711	consuming sweets	1696:1711	consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries	1696:1825	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	8	64	theme	consuming	1696:1704	arg1	≤					1716:1716	p ≤ 0.001	1714:1722	p ≤ 0.001 for frequent and repeated consumption of sweets during the day	1714:1785	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	8	65	theme	intensity	1582:1590	arg1	existence					1478:1486	the existence	1474:1486	the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries	1474:1825	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	1	66	theme	study	157:161	arg1	aim					146:148	The aim	142:148	The aim of the study	142:161	The aim of the study was to verify the hypothesis about differences in sweet taste perception in the group of preschool children with and without caries, and to determine its relationship with cariogenic microbiota and the frequency of sweets consumption in children.
32858903	10	67	theme	preferences	2049:2059	arg1	use					2036:2038	the future use	2025:2038	the future use of taste preferences in children	2025:2071	The results of this study suggest the future use of taste preferences in children as a diagnostic tool for the early detection of increased susceptibility to caries through microbial dysbiosis towards specific species of microorganisms.
32858903	10	67	theme	preferences	2049:2059	arg1	tool					2089:2092	a diagnostic tool	2076:2092	a diagnostic tool for the early detection of increased susceptibility to caries through microbial dysbiosis towards specific species of microorganisms	2076:2225	The results of this study suggest the future use of taste preferences in children as a diagnostic tool for the early detection of increased susceptibility to caries through microbial dysbiosis towards specific species of microorganisms.
32858903	6	68	theme	sucrose	1193:1199	arg1	concentration					1210:1222	the preferred sucrose solution concentration	1179:1222	the preferred sucrose solution concentration was >4 g/L	1179:1233	The sweet taste perception of children with caries was characterized by a lower susceptibility to sucrose (the preferred sucrose solution concentration was >4 g/L) compared to children without caries (in the range ≤ 4 g/L, p = 0.0015, chi-square test).
32858903	6	69	theme	preferred	1183:1191	arg1	concentration					1210:1222	the preferred sucrose solution concentration	1179:1222	the preferred sucrose solution concentration was >4 g/L	1179:1233	The sweet taste perception of children with caries was characterized by a lower susceptibility to sucrose (the preferred sucrose solution concentration was >4 g/L) compared to children without caries (in the range ≤ 4 g/L, p = 0.0015, chi-square test).
32858903	3	70	theme	Assessment	697:706	arg1	System					708:713	the decayed, missing, filled in primary teeth index (dmft) and the International Caries Detection and Assessment System	595:713	System	708:713	The study consisted of collecting questionnaire data and assessment of dental status using the decayed, missing, filled in primary teeth index (dmft) and the International Caries Detection and Assessment System (ICDAS II).
32858903	8	71	theme	process	1610:1616	arg1	correlation					1509:1519	a strong positive correlation	1491:1519	a strong positive correlation between the perception of sweet taste and the occurrence	1491:1576	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	8	71	theme	process	1610:1616	arg1	intensity					1582:1590	intensity	1582:1590	intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries	1582:1825	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	6	72	theme	taste	1082:1086	arg1	perception					1088:1097	The sweet taste perception	1072:1097	The sweet taste perception of children with caries	1072:1121	The sweet taste perception of children with caries was characterized by a lower susceptibility to sucrose (the preferred sucrose solution concentration was >4 g/L) compared to children without caries (in the range ≤ 4 g/L, p = 0.0015, chi-square test).
32858903	4	73	theme	preferences	856:866	arg1	threshold					888:896	the sensitivity threshold	872:896	the sensitivity threshold for a given taste	872:914	The evaluation of sweet taste perception was carried out using a specific method that simultaneously assessed the level of taste preferences and the sensitivity threshold for a given taste.
32858903	4	73	theme	preferences	856:866	arg1	level					841:845	the level	837:845	the level of taste preferences	837:866	The evaluation of sweet taste perception was carried out using a specific method that simultaneously assessed the level of taste preferences and the sensitivity threshold for a given taste.
32858903	10	74	theme	susceptibility	2131:2144	arg1	detection					2108:2116	the early detection	2098:2116	the early detection of increased susceptibility to caries through microbial dysbiosis towards specific species of microorganisms	2098:2225	The results of this study suggest the future use of taste preferences in children as a diagnostic tool for the early detection of increased susceptibility to caries through microbial dysbiosis towards specific species of microorganisms.
32858903	5	75	theme	oral	1030:1033	arg1	microbiota					1035:1044	the oral microbiota	1026:1044	the oral microbiota of the examined children	1026:1069	The microbiological analysis consisted of the assessment of the quantitative and qualitative compositions of the oral microbiota of the examined children.
32858903	3	76	theme	questionnaire	538:550	arg1	data					552:555	questionnaire data	538:555	questionnaire data	538:555	The study consisted of collecting questionnaire data and assessment of dental status using the decayed, missing, filled in primary teeth index (dmft) and the International Caries Detection and Assessment System (ICDAS II).
32858903	1	77	from	differences	198:208	arg1	perception					225:234	sweet taste perception	213:234	sweet taste perception in the group of preschool children with and without caries	213:293	The aim of the study was to verify the hypothesis about differences in sweet taste perception in the group of preschool children with and without caries, and to determine its relationship with cariogenic microbiota and the frequency of sweets consumption in children.
32858903	8	78	from	children	1806:1813	arg1	frequency					1683:1691	the frequency	1679:1691	the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries	1679:1825	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	8	78	from	children	1806:1813	arg1	process					1610:1616	the cariogenic process	1595:1616	the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II)	1595:1665	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	6	79	with	children	1102:1109	arg1	caries					1116:1121	caries	1116:1121	caries	1116:1121	The sweet taste perception of children with caries was characterized by a lower susceptibility to sucrose (the preferred sucrose solution concentration was >4 g/L) compared to children without caries (in the range ≤ 4 g/L, p = 0.0015, chi-square test).
32858903	10	80	theme	diagnostic	2078:2087	arg1	use					2036:2038	the future use	2025:2038	the future use of taste preferences in children	2025:2071	The results of this study suggest the future use of taste preferences in children as a diagnostic tool for the early detection of increased susceptibility to caries through microbial dysbiosis towards specific species of microorganisms.
32858903	10	80	theme	diagnostic	2078:2087	arg1	tool					2089:2092	a diagnostic tool	2076:2092	a diagnostic tool for the early detection of increased susceptibility to caries through microbial dysbiosis towards specific species of microorganisms	2076:2225	The results of this study suggest the future use of taste preferences in children as a diagnostic tool for the early detection of increased susceptibility to caries through microbial dysbiosis towards specific species of microorganisms.
32858903	8	81	theme	sweets	1765:1770	arg1	consumption					1750:1760	frequent and repeated consumption	1728:1760	frequent and repeated consumption of sweets during the day	1728:1785	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	5	82	theme	quantitative	981:992	arg1	compositions					1010:1021	the quantitative and qualitative compositions	977:1021	the quantitative and qualitative compositions of the oral microbiota of the examined children	977:1069	The microbiological analysis consisted of the assessment of the quantitative and qualitative compositions of the oral microbiota of the examined children.
32858903	3	83	theme	status	582:587	arg1	assessment					561:570	assessment	561:570	assessment of dental status	561:587	The study consisted of collecting questionnaire data and assessment of dental status using the decayed, missing, filled in primary teeth index (dmft) and the International Caries Detection and Assessment System (ICDAS II).
32858903	3	83	theme	status	582:587	arg1	data					552:555	questionnaire data	538:555	questionnaire data	538:555	The study consisted of collecting questionnaire data and assessment of dental status using the decayed, missing, filled in primary teeth index (dmft) and the International Caries Detection and Assessment System (ICDAS II).
32858903	0	84	from	Profile	99:105	arg1	Children					120:127	Preschool Children	110:127	Preschool Children with Caries	110:139	Differences in Sweet Taste Perception and Its Association with the Streptococcus mutans Cariogenic Profile in Preschool Children with Caries.
32858903	6	85	theme	lower	1146:1150	arg1	susceptibility					1152:1165	a lower susceptibility	1144:1165	a lower susceptibility to sucrose (the preferred sucrose solution concentration was >4 g/L) compared to children without caries (in the range ≤ 4 g/L, p = 0.0015, chi-square test)	1144:1322	The sweet taste perception of children with caries was characterized by a lower susceptibility to sucrose (the preferred sucrose solution concentration was >4 g/L) compared to children without caries (in the range ≤ 4 g/L, p = 0.0015, chi-square test).
32858903	1	86	from	children	400:407	arg1	microbiota					346:355	cariogenic microbiota	335:355	cariogenic microbiota	335:355	The aim of the study was to verify the hypothesis about differences in sweet taste perception in the group of preschool children with and without caries, and to determine its relationship with cariogenic microbiota and the frequency of sweets consumption in children.
32858903	1	86	from	children	400:407	arg1	frequency					365:373	the frequency	361:373	the frequency of sweets consumption in children	361:407	The aim of the study was to verify the hypothesis about differences in sweet taste perception in the group of preschool children with and without caries, and to determine its relationship with cariogenic microbiota and the frequency of sweets consumption in children.
32858903	5	87	theme	qualitative	998:1008	arg1	compositions					1010:1021	the quantitative and qualitative compositions	977:1021	the quantitative and qualitative compositions of the oral microbiota of the examined children	977:1069	The microbiological analysis consisted of the assessment of the quantitative and qualitative compositions of the oral microbiota of the examined children.
32858903	10	88	theme	increased	2121:2129	arg1	susceptibility					2131:2144	increased susceptibility	2121:2144	increased susceptibility to caries	2121:2154	The results of this study suggest the future use of taste preferences in children as a diagnostic tool for the early detection of increased susceptibility to caries through microbial dysbiosis towards specific species of microorganisms.
32858903	8	89	theme	Spearman	1788:1795	arg1	test					1797:1800	Spearman test	1788:1800	Spearman test	1788:1800	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	1	90	from	consumption	385:395	arg1	children					400:407	children	400:407	children	400:407	The aim of the study was to verify the hypothesis about differences in sweet taste perception in the group of preschool children with and without caries, and to determine its relationship with cariogenic microbiota and the frequency of sweets consumption in children.
32858903	9	91	theme	=	1962:1962	arg1	CI					1975:1976	= 10.21; 95% CI 3.11-33.44	1962:1987	= 10.21; 95% CI 3.11-33.44	1962:1987	Additionally, children with an elevated sucrose taste threshold were more than 10-times more likely to develop S. mutans presence (OR = 10.21; 95% CI 3.11-33.44).
32858903	8	92	theme	=	1645:1645	arg1	p					1643:1643	p = 0.012	1643:1651	p = 0.012 for ICDAS II	1643:1664	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	1	93	with	relationship	317:328	arg1	microbiota					346:355	cariogenic microbiota	335:355	cariogenic microbiota	335:355	The aim of the study was to verify the hypothesis about differences in sweet taste perception in the group of preschool children with and without caries, and to determine its relationship with cariogenic microbiota and the frequency of sweets consumption in children.
32858903	1	93	with	relationship	317:328	arg1	frequency					365:373	the frequency	361:373	the frequency of sweets consumption in children	361:407	The aim of the study was to verify the hypothesis about differences in sweet taste perception in the group of preschool children with and without caries, and to determine its relationship with cariogenic microbiota and the frequency of sweets consumption in children.
32858903	0	94	theme	Preschool	110:118	arg1	Children					120:127	Preschool Children	110:127	Preschool Children with Caries	110:139	Differences in Sweet Taste Perception and Its Association with the Streptococcus mutans Cariogenic Profile in Preschool Children with Caries.
32858903	1	95	theme	cariogenic	335:344	arg1	microbiota					346:355	cariogenic microbiota	335:355	cariogenic microbiota	335:355	The aim of the study was to verify the hypothesis about differences in sweet taste perception in the group of preschool children with and without caries, and to determine its relationship with cariogenic microbiota and the frequency of sweets consumption in children.
32858903	10	96	theme	study	2011:2015	arg1	results					1995:2001	The results	1991:2001	The results of this study	1991:2015	The results of this study suggest the future use of taste preferences in children as a diagnostic tool for the early detection of increased susceptibility to caries through microbial dysbiosis towards specific species of microorganisms.
32858903	1	97	theme	sweet	213:217	arg1	perception					225:234	sweet taste perception	213:234	sweet taste perception in the group of preschool children with and without caries	213:293	The aim of the study was to verify the hypothesis about differences in sweet taste perception in the group of preschool children with and without caries, and to determine its relationship with cariogenic microbiota and the frequency of sweets consumption in children.
32858903	6	98	theme	≤	1286:1286	arg1	g/L					1290:1292	the range ≤ 4 g/L	1276:1292	the range ≤ 4 g/L	1276:1292	The sweet taste perception of children with caries was characterized by a lower susceptibility to sucrose (the preferred sucrose solution concentration was >4 g/L) compared to children without caries (in the range ≤ 4 g/L, p = 0.0015, chi-square test).
32858903	1	99	from	microbiota	346:355	arg1	children					400:407	children	400:407	children	400:407	The aim of the study was to verify the hypothesis about differences in sweet taste perception in the group of preschool children with and without caries, and to determine its relationship with cariogenic microbiota and the frequency of sweets consumption in children.
32858903	5	100	theme	examined	1053:1060	arg1	children					1062:1069	the examined children	1049:1069	the examined children	1049:1069	The microbiological analysis consisted of the assessment of the quantitative and qualitative compositions of the oral microbiota of the examined children.
32858903	6	101	dep	0.0015	1299:1304	arg1	test					1318:1321	chi-square test	1307:1321	chi-square test	1307:1321	The sweet taste perception of children with caries was characterized by a lower susceptibility to sucrose (the preferred sucrose solution concentration was >4 g/L) compared to children without caries (in the range ≤ 4 g/L, p = 0.0015, chi-square test).
32858903	10	102	theme	specific	2192:2199	arg1	species					2201:2207	specific species	2192:2207	specific species of microorganisms	2192:2225	The results of this study suggest the future use of taste preferences in children as a diagnostic tool for the early detection of increased susceptibility to caries through microbial dysbiosis towards specific species of microorganisms.
32858903	0	103	theme	Taste	21:25	arg1	Perception					27:36	Sweet Taste Perception	15:36	Sweet Taste Perception	15:36	Differences in Sweet Taste Perception and Its Association with the Streptococcus mutans Cariogenic Profile in Preschool Children with Caries.
32858903	1	104	theme	children	262:269	arg1	group					243:247	the group	239:247	the group of preschool children with and without caries	239:293	The aim of the study was to verify the hypothesis about differences in sweet taste perception in the group of preschool children with and without caries, and to determine its relationship with cariogenic microbiota and the frequency of sweets consumption in children.
32858903	1	104	theme	children	262:269	arg1	children					262:269	preschool children	252:269	preschool children with and without caries	252:293	The aim of the study was to verify the hypothesis about differences in sweet taste perception in the group of preschool children with and without caries, and to determine its relationship with cariogenic microbiota and the frequency of sweets consumption in children.
32858903	10	105	theme	microbial	2164:2172	arg1	dysbiosis					2174:2182	microbial dysbiosis	2164:2182	microbial dysbiosis towards specific species of microorganisms	2164:2225	The results of this study suggest the future use of taste preferences in children as a diagnostic tool for the early detection of increased susceptibility to caries through microbial dysbiosis towards specific species of microorganisms.
32858903	8	106	from	frequency	1683:1691	arg1	children					1806:1813	children	1806:1813	children with caries	1806:1825	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	7	107	dep	meals	1396:1400	arg1	test					1426:1429	chi-square test	1415:1429	chi-square test	1415:1429	A similar relationship was also observed for frequent snacking between meals (p = 0.0038, chi-square test).
32858903	7	107	dep	meals	1396:1400	arg1	p					1403:1403	p	1403:1403	p = 0.0038	1403:1412	A similar relationship was also observed for frequent snacking between meals (p = 0.0038, chi-square test).
32858903	8	108	theme	variables	1457:1465	arg1	analysis					1437:1444	The analysis	1433:1444	The analysis of studied variables	1433:1465	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	9	109	theme	sucrose	1868:1874	arg1	threshold					1882:1890	an elevated sucrose taste threshold	1856:1890	an elevated sucrose taste threshold	1856:1890	Additionally, children with an elevated sucrose taste threshold were more than 10-times more likely to develop S. mutans presence (OR = 10.21; 95% CI 3.11-33.44).
32858903	6	110	theme	range	1280:1284	arg1	g/L					1290:1292	the range ≤ 4 g/L	1276:1292	the range ≤ 4 g/L	1276:1292	The sweet taste perception of children with caries was characterized by a lower susceptibility to sucrose (the preferred sucrose solution concentration was >4 g/L) compared to children without caries (in the range ≤ 4 g/L, p = 0.0015, chi-square test).
32858903	6	111	theme	chi-square	1307:1316	arg1	test					1318:1321	chi-square test	1307:1321	chi-square test	1307:1321	The sweet taste perception of children with caries was characterized by a lower susceptibility to sucrose (the preferred sucrose solution concentration was >4 g/L) compared to children without caries (in the range ≤ 4 g/L, p = 0.0015, chi-square test).
32858903	7	112	theme	chi-square	1415:1424	arg1	test					1426:1429	chi-square test	1415:1429	chi-square test	1415:1429	A similar relationship was also observed for frequent snacking between meals (p = 0.0038, chi-square test).
32858903	7	112	theme	chi-square	1415:1424	arg1	p					1403:1403	p	1403:1403	p = 0.0038	1403:1412	A similar relationship was also observed for frequent snacking between meals (p = 0.0038, chi-square test).
32858903	6	113	theme	sweet	1076:1080	arg1	perception					1088:1097	The sweet taste perception	1072:1097	The sweet taste perception of children with caries	1072:1121	The sweet taste perception of children with caries was characterized by a lower susceptibility to sucrose (the preferred sucrose solution concentration was >4 g/L) compared to children without caries (in the range ≤ 4 g/L, p = 0.0015, chi-square test).
32858903	4	114	theme	taste	751:755	arg1	perception					757:766	sweet taste perception	745:766	sweet taste perception	745:766	The evaluation of sweet taste perception was carried out using a specific method that simultaneously assessed the level of taste preferences and the sensitivity threshold for a given taste.
32858903	0	115	with	Association	46:56	arg1	Profile					99:105	the Streptococcus mutans Cariogenic Profile	63:105	the Streptococcus mutans Cariogenic Profile in Preschool Children with Caries	63:139	Differences in Sweet Taste Perception and Its Association with the Streptococcus mutans Cariogenic Profile in Preschool Children with Caries.
32858903	8	116	theme	taste	1553:1557	arg1	perception					1533:1542	the perception	1529:1542	the perception of sweet taste	1529:1557	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	8	116	theme	taste	1553:1557	arg1	occurrence					1567:1576	the occurrence	1563:1576	the occurrence	1563:1576	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	3	117	dep	decayed	599:605	arg1	filled					617:622	filled	617:622	filled	617:622	The study consisted of collecting questionnaire data and assessment of dental status using the decayed, missing, filled in primary teeth index (dmft) and the International Caries Detection and Assessment System (ICDAS II).
32858903	3	117	dep	decayed	599:605	arg1	missing					608:614	missing	608:614	missing	608:614	The study consisted of collecting questionnaire data and assessment of dental status using the decayed, missing, filled in primary teeth index (dmft) and the International Caries Detection and Assessment System (ICDAS II).
32858903	8	118	theme	strong	1493:1498	arg1	correlation					1509:1519	a strong positive correlation	1491:1519	a strong positive correlation between the perception of sweet taste and the occurrence	1491:1576	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32858903	8	119	from	process	1610:1616	arg1	children					1806:1813	children	1806:1813	children with caries	1806:1825	The analysis of studied variables showed the existence of a strong positive correlation between the perception of sweet taste and the occurrence and intensity of the cariogenic process (p = 0.007 for dmft; and p = 0.012 for ICDAS II), as well as the frequency of consuming sweets (p ≤ 0.001 for frequent and repeated consumption of sweets during the day, Spearman test) in children with caries.
32394442	2	0	theme	biological	434:443	arg1	research					445:452	relevant biological research	425:452	relevant biological research on the anti-inflammatory capacity of COO	425:493	However, there has been a lack of relevant biological research on the anti-inflammatory capacity of COO, and the specific bioactive lipid phytochemicals contributing to the anti-inflammatory effect need further research.
32394442	6	1	theme	suppressive	983:993	arg1	effect					995:1000	a strong suppressive effect	974:1000	a strong suppressive effect on the expression of proinflammatory cytokines such as Tnf-α and Il-6	974:1070	The enzyme immunoassay confirmed that COO also exhibited a strong suppressive effect on the expression of proinflammatory cytokines such as Tnf-α and Il-6.
32394442	2	2	theme	anti-inflammatory	564:580	arg1	effect					582:587	the anti-inflammatory effect	560:587	the anti-inflammatory effect	560:587	However, there has been a lack of relevant biological research on the anti-inflammatory capacity of COO, and the specific bioactive lipid phytochemicals contributing to the anti-inflammatory effect need further research.
32394442	2	3	from	lack	417:420	arg1	capacity					479:486	the anti-inflammatory capacity	457:486	the anti-inflammatory capacity of COO	457:493	However, there has been a lack of relevant biological research on the anti-inflammatory capacity of COO, and the specific bioactive lipid phytochemicals contributing to the anti-inflammatory effect need further research.
32394442	8	4	theme	COO	1321:1323	arg1	extracts					1309:1316	the polyphenol extracts	1294:1316	the polyphenol extracts of COO	1294:1323	It was interestingly demonstrated that the polyphenol extracts of COO play a vital role in its anti-inflammatory properties.
32394442	7	5	from	phytochemicals	1160:1173	arg1	COO					1178:1180	COO	1178:1180	COO	1178:1180	To further explore the correlation between the anti-inflammatory effects and the lipid phytochemicals in COO, 10 samples were collected and screened for their chemical compositions.
32394442	2	6	theme	relevant	425:432	arg1	research					445:452	relevant biological research	425:452	relevant biological research on the anti-inflammatory capacity of COO	425:493	However, there has been a lack of relevant biological research on the anti-inflammatory capacity of COO, and the specific bioactive lipid phytochemicals contributing to the anti-inflammatory effect need further research.
32394442	3	7	theme	macrophages	641:651	arg1	model					653:657	the RAW 264.7 macrophages model	627:657	the RAW 264.7 macrophages model	627:657	In this study, the RAW 264.7 macrophages model was used to investigate the anti-inflammatory capacity of COO.
32394442	4	8	theme	nitric	838:843	arg1	NO					852:853	NO.	852:854	NO.	852:854	Our data showed that 33-200 μg/mL COO markedly inhibited the lipopolysaccharide lipopolysaccharide (LPS)-stimulated nitric oxide (NO.)
32394442	4	8	theme	nitric	838:843	arg1	oxide					845:849	the lipopolysaccharide lipopolysaccharide (LPS)-stimulated nitric oxide	779:849	the lipopolysaccharide lipopolysaccharide (LPS)-stimulated nitric oxide (NO.)	779:855	Our data showed that 33-200 μg/mL COO markedly inhibited the lipopolysaccharide lipopolysaccharide (LPS)-stimulated nitric oxide (NO.)
32394442	1	9	theme	Camellia	176:183	arg1	oil					232:234	a common edible oil	216:234	not only a common edible oil but also a traditional remedy widely applied to ameliorate a variety of illnesses associated with inflammation, such as mouth ulcers, thrush, eczema, etc	207:388	In China, Camellia oleifera oil (COO) is not only a common edible oil but also a traditional remedy widely applied to ameliorate a variety of illnesses associated with inflammation, such as mouth ulcers, thrush, eczema, etc.
32394442	1	9	theme	Camellia	176:183	arg1	oil					194:196	Camellia oleifera oil	176:196	Camellia oleifera oil (COO)	176:202	In China, Camellia oleifera oil (COO) is not only a common edible oil but also a traditional remedy widely applied to ameliorate a variety of illnesses associated with inflammation, such as mouth ulcers, thrush, eczema, etc.
32394442	1	9	theme	Camellia	176:183	arg1	COO					199:201	COO	199:201	COO	199:201	In China, Camellia oleifera oil (COO) is not only a common edible oil but also a traditional remedy widely applied to ameliorate a variety of illnesses associated with inflammation, such as mouth ulcers, thrush, eczema, etc.
32394442	0	10	theme	Effect	103:108	arg1	Analysis					0:7	Analysis	0:7	Analysis of Phytochemical Composition of Camellia oleifera Oil	0:61	Analysis of Phytochemical Composition of Camellia oleifera Oil and Evaluation of its Anti-Inflammatory Effect in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages.
32394442	0	10	theme	Effect	103:108	arg1	Evaluation					67:76	Evaluation	67:76	Evaluation of its Anti-Inflammatory Effect in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages	67:163	Analysis of Phytochemical Composition of Camellia oleifera Oil and Evaluation of its Anti-Inflammatory Effect in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages.
32394442	0	11	from	Analysis	0:7	arg1	Macrophages					153:163	Lipopolysaccharide-Stimulated RAW 264.7 Macrophages	113:163	Lipopolysaccharide-Stimulated RAW 264.7 Macrophages	113:163	Analysis of Phytochemical Composition of Camellia oleifera Oil and Evaluation of its Anti-Inflammatory Effect in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages.
32394442	11	12	dep	utilization	1747:1757	arg1	the					1729:1731	the	1729:1731	the	1729:1731	This may provide a scientific basis for the comprehensive utilization and development of COO and related functional foods.
32394442	0	13	theme	Anti-Inflammatory	85:101	arg1	Effect					103:108	its Anti-Inflammatory Effect	81:108	its Anti-Inflammatory Effect	81:108	Analysis of Phytochemical Composition of Camellia oleifera Oil and Evaluation of its Anti-Inflammatory Effect in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages.
32394442	6	14	from	effect	995:1000	arg1	expression					1009:1018	the expression	1005:1018	the expression of proinflammatory cytokines such as Tnf-α and Il-6	1005:1070	The enzyme immunoassay confirmed that COO also exhibited a strong suppressive effect on the expression of proinflammatory cytokines such as Tnf-α and Il-6.
32394442	7	15	theme	anti-inflammatory	1120:1136	arg1	effects					1138:1144	the anti-inflammatory effects	1116:1144	the anti-inflammatory effects	1116:1144	To further explore the correlation between the anti-inflammatory effects and the lipid phytochemicals in COO, 10 samples were collected and screened for their chemical compositions.
32394442	1	16	theme	traditional	247:257	arg1	remedy					259:264	a traditional remedy	245:264	not only a common edible oil but also a traditional remedy widely applied to ameliorate a variety of illnesses associated with inflammation, such as mouth ulcers, thrush, eczema, etc	207:388	In China, Camellia oleifera oil (COO) is not only a common edible oil but also a traditional remedy widely applied to ameliorate a variety of illnesses associated with inflammation, such as mouth ulcers, thrush, eczema, etc.
32394442	0	17	theme	Lipopolysaccharide-Stimulated	113:141	arg1	Macrophages					153:163	Lipopolysaccharide-Stimulated RAW 264.7 Macrophages	113:163	Lipopolysaccharide-Stimulated RAW 264.7 Macrophages	113:163	Analysis of Phytochemical Composition of Camellia oleifera Oil and Evaluation of its Anti-Inflammatory Effect in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages.
32394442	3	18	theme	anti-inflammatory	687:703	arg1	capacity					705:712	the anti-inflammatory capacity	683:712	the anti-inflammatory capacity of COO	683:719	In this study, the RAW 264.7 macrophages model was used to investigate the anti-inflammatory capacity of COO.
32394442	7	19	from	effects	1138:1144	arg1	COO					1178:1180	COO	1178:1180	COO	1178:1180	To further explore the correlation between the anti-inflammatory effects and the lipid phytochemicals in COO, 10 samples were collected and screened for their chemical compositions.
32394442	1	20	theme	oleifera	185:192	arg1	oil					232:234	a common edible oil	216:234	not only a common edible oil but also a traditional remedy widely applied to ameliorate a variety of illnesses associated with inflammation, such as mouth ulcers, thrush, eczema, etc	207:388	In China, Camellia oleifera oil (COO) is not only a common edible oil but also a traditional remedy widely applied to ameliorate a variety of illnesses associated with inflammation, such as mouth ulcers, thrush, eczema, etc.
32394442	1	20	theme	oleifera	185:192	arg1	oil					194:196	Camellia oleifera oil	176:196	Camellia oleifera oil (COO)	176:202	In China, Camellia oleifera oil (COO) is not only a common edible oil but also a traditional remedy widely applied to ameliorate a variety of illnesses associated with inflammation, such as mouth ulcers, thrush, eczema, etc.
32394442	1	20	theme	oleifera	185:192	arg1	COO					199:201	COO	199:201	COO	199:201	In China, Camellia oleifera oil (COO) is not only a common edible oil but also a traditional remedy widely applied to ameliorate a variety of illnesses associated with inflammation, such as mouth ulcers, thrush, eczema, etc.
32394442	10	21	theme	anti-inflammatory	1579:1595	arg1	potential					1597:1605	the anti-inflammatory potential	1575:1605	the anti-inflammatory potential of COO	1575:1612	Our research confirms the anti-inflammatory potential of COO and highlights that the main functional ingredient is polyphenol extracts.
32394442	2	22	theme	further	594:600	arg1	research					602:609	further research	594:609	further research	594:609	However, there has been a lack of relevant biological research on the anti-inflammatory capacity of COO, and the specific bioactive lipid phytochemicals contributing to the anti-inflammatory effect need further research.
32394442	8	23	theme	vital	1332:1336	arg1	role					1338:1341	a vital role	1330:1341	a vital role	1330:1341	It was interestingly demonstrated that the polyphenol extracts of COO play a vital role in its anti-inflammatory properties.
32394442	2	24	theme	anti-inflammatory	461:477	arg1	capacity					479:486	the anti-inflammatory capacity	457:486	the anti-inflammatory capacity of COO	457:493	However, there has been a lack of relevant biological research on the anti-inflammatory capacity of COO, and the specific bioactive lipid phytochemicals contributing to the anti-inflammatory effect need further research.
32394442	7	25	theme	lipid	1154:1158	arg1	phytochemicals					1160:1173	the lipid phytochemicals	1150:1173	the lipid phytochemicals in COO	1150:1180	To further explore the correlation between the anti-inflammatory effects and the lipid phytochemicals in COO, 10 samples were collected and screened for their chemical compositions.
32394442	1	26	theme	mouth	356:360	arg1	thrush					370:375	thrush	370:375	thrush	370:375	In China, Camellia oleifera oil (COO) is not only a common edible oil but also a traditional remedy widely applied to ameliorate a variety of illnesses associated with inflammation, such as mouth ulcers, thrush, eczema, etc.
32394442	1	26	theme	mouth	356:360	arg1	ulcers					362:367	mouth ulcers	356:367	mouth ulcers	356:367	In China, Camellia oleifera oil (COO) is not only a common edible oil but also a traditional remedy widely applied to ameliorate a variety of illnesses associated with inflammation, such as mouth ulcers, thrush, eczema, etc.
32394442	1	26	theme	mouth	356:360	arg1	etc					386:388	etc	386:388	etc	386:388	In China, Camellia oleifera oil (COO) is not only a common edible oil but also a traditional remedy widely applied to ameliorate a variety of illnesses associated with inflammation, such as mouth ulcers, thrush, eczema, etc.
32394442	1	26	theme	mouth	356:360	arg1	eczema					378:383	eczema	378:383	eczema	378:383	In China, Camellia oleifera oil (COO) is not only a common edible oil but also a traditional remedy widely applied to ameliorate a variety of illnesses associated with inflammation, such as mouth ulcers, thrush, eczema, etc.
32394442	1	27	from	remedy	259:264	arg1	China					169:173	China	169:173	China	169:173	In China, Camellia oleifera oil (COO) is not only a common edible oil but also a traditional remedy widely applied to ameliorate a variety of illnesses associated with inflammation, such as mouth ulcers, thrush, eczema, etc.
32394442	11	28	theme	related	1786:1792	arg1	foods					1805:1809	COO and related functional foods	1778:1809	COO and related functional foods	1778:1809	This may provide a scientific basis for the comprehensive utilization and development of COO and related functional foods.
32394442	5	29	theme	Cox-2	899:903	arg1	expression					905:914	Cox-2 expression	899:914	Cox-2 expression	899:914	secretion via the suppression of Nos2 and Cox-2 expression.
32394442	1	30	from	oil	232:234	arg1	China					169:173	China	169:173	China	169:173	In China, Camellia oleifera oil (COO) is not only a common edible oil but also a traditional remedy widely applied to ameliorate a variety of illnesses associated with inflammation, such as mouth ulcers, thrush, eczema, etc.
32394442	3	31	theme	COO	717:719	arg1	capacity					705:712	the anti-inflammatory capacity	683:712	the anti-inflammatory capacity of COO	683:719	In this study, the RAW 264.7 macrophages model was used to investigate the anti-inflammatory capacity of COO.
32394442	4	32	theme	-stimulated	826:836	arg1	NO					852:853	NO.	852:854	NO.	852:854	Our data showed that 33-200 μg/mL COO markedly inhibited the lipopolysaccharide lipopolysaccharide (LPS)-stimulated nitric oxide (NO.)
32394442	4	32	theme	-stimulated	826:836	arg1	oxide					845:849	the lipopolysaccharide lipopolysaccharide (LPS)-stimulated nitric oxide	779:849	the lipopolysaccharide lipopolysaccharide (LPS)-stimulated nitric oxide (NO.)	779:855	Our data showed that 33-200 μg/mL COO markedly inhibited the lipopolysaccharide lipopolysaccharide (LPS)-stimulated nitric oxide (NO.)
32394442	10	33	theme	polyphenol	1668:1677	arg1	ingredient					1654:1663	the main functional ingredient	1634:1663	the main functional ingredient	1634:1663	Our research confirms the anti-inflammatory potential of COO and highlights that the main functional ingredient is polyphenol extracts.
32394442	10	33	theme	polyphenol	1668:1677	arg1	extracts					1679:1686	polyphenol extracts	1668:1686	polyphenol extracts	1668:1686	Our research confirms the anti-inflammatory potential of COO and highlights that the main functional ingredient is polyphenol extracts.
32394442	5	34	theme	expression	905:914	arg1	suppression					875:885	the suppression	871:885	the suppression of Nos2 and Cox-2 expression	871:914	secretion via the suppression of Nos2 and Cox-2 expression.
32394442	6	35	theme	cytokines	1039:1047	arg1	expression					1009:1018	the expression	1005:1018	the expression of proinflammatory cytokines such as Tnf-α and Il-6	1005:1070	The enzyme immunoassay confirmed that COO also exhibited a strong suppressive effect on the expression of proinflammatory cytokines such as Tnf-α and Il-6.
32394442	0	36	theme	Composition	26:36	arg1	Analysis					0:7	Analysis	0:7	Analysis of Phytochemical Composition of Camellia oleifera Oil	0:61	Analysis of Phytochemical Composition of Camellia oleifera Oil and Evaluation of its Anti-Inflammatory Effect in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages.
32394442	0	36	theme	Composition	26:36	arg1	Evaluation					67:76	Evaluation	67:76	Evaluation of its Anti-Inflammatory Effect in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages	67:163	Analysis of Phytochemical Composition of Camellia oleifera Oil and Evaluation of its Anti-Inflammatory Effect in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages.
32394442	11	37	theme	functional	1794:1803	arg1	foods					1805:1809	COO and related functional foods	1778:1809	COO and related functional foods	1778:1809	This may provide a scientific basis for the comprehensive utilization and development of COO and related functional foods.
32394442	6	38	theme	proinflammatory	1023:1037	arg1	Il-6					1067:1070	Il-6	1067:1070	Il-6	1067:1070	The enzyme immunoassay confirmed that COO also exhibited a strong suppressive effect on the expression of proinflammatory cytokines such as Tnf-α and Il-6.
32394442	6	38	theme	proinflammatory	1023:1037	arg1	cytokines					1039:1047	proinflammatory cytokines	1023:1047	proinflammatory cytokines such as Tnf-α and Il-6	1023:1070	The enzyme immunoassay confirmed that COO also exhibited a strong suppressive effect on the expression of proinflammatory cytokines such as Tnf-α and Il-6.
32394442	6	38	theme	proinflammatory	1023:1037	arg1	Tnf-α					1057:1061	Tnf-α	1057:1061	Tnf-α	1057:1061	The enzyme immunoassay confirmed that COO also exhibited a strong suppressive effect on the expression of proinflammatory cytokines such as Tnf-α and Il-6.
32394442	0	39	theme	Phytochemical	12:24	arg1	Composition					26:36	Phytochemical Composition	12:36	Phytochemical Composition of Camellia oleifera Oil	12:61	Analysis of Phytochemical Composition of Camellia oleifera Oil and Evaluation of its Anti-Inflammatory Effect in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages.
32394442	11	40	theme	COO	1778:1780	arg1	foods					1805:1809	COO and related functional foods	1778:1809	COO and related functional foods	1778:1809	This may provide a scientific basis for the comprehensive utilization and development of COO and related functional foods.
32394442	0	41	theme	RAW	143:145	arg1	Macrophages					153:163	Lipopolysaccharide-Stimulated RAW 264.7 Macrophages	113:163	Lipopolysaccharide-Stimulated RAW 264.7 Macrophages	113:163	Analysis of Phytochemical Composition of Camellia oleifera Oil and Evaluation of its Anti-Inflammatory Effect in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages.
32394442	8	42	theme	anti-inflammatory	1350:1366	arg1	properties					1368:1377	its anti-inflammatory properties	1346:1377	its anti-inflammatory properties	1346:1377	It was interestingly demonstrated that the polyphenol extracts of COO play a vital role in its anti-inflammatory properties.
32394442	0	43	theme	Camellia	41:48	arg1	Oil					59:61	Camellia oleifera Oil	41:61	Camellia oleifera Oil	41:61	Analysis of Phytochemical Composition of Camellia oleifera Oil and Evaluation of its Anti-Inflammatory Effect in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages.
32394442	6	44	theme	enzyme	921:926	arg1	immunoassay					928:938	The enzyme immunoassay	917:938	The enzyme immunoassay	917:938	The enzyme immunoassay confirmed that COO also exhibited a strong suppressive effect on the expression of proinflammatory cytokines such as Tnf-α and Il-6.
32394442	10	45	theme	functional	1643:1652	arg1	ingredient					1654:1663	the main functional ingredient	1634:1663	the main functional ingredient	1634:1663	Our research confirms the anti-inflammatory potential of COO and highlights that the main functional ingredient is polyphenol extracts.
32394442	10	45	theme	functional	1643:1652	arg1	extracts					1679:1686	polyphenol extracts	1668:1686	polyphenol extracts	1668:1686	Our research confirms the anti-inflammatory potential of COO and highlights that the main functional ingredient is polyphenol extracts.
32394442	2	46	theme	COO	491:493	arg1	capacity					479:486	the anti-inflammatory capacity	457:486	the anti-inflammatory capacity of COO	457:493	However, there has been a lack of relevant biological research on the anti-inflammatory capacity of COO, and the specific bioactive lipid phytochemicals contributing to the anti-inflammatory effect need further research.
32394442	0	47	from	Evaluation	67:76	arg1	Macrophages					153:163	Lipopolysaccharide-Stimulated RAW 264.7 Macrophages	113:163	Lipopolysaccharide-Stimulated RAW 264.7 Macrophages	113:163	Analysis of Phytochemical Composition of Camellia oleifera Oil and Evaluation of its Anti-Inflammatory Effect in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages.
32394442	3	48	used	used	663:666	arg2	model					653:657	the RAW 264.7 macrophages model	627:657	the RAW 264.7 macrophages model	627:657	In this study, the RAW 264.7 macrophages model was used to investigate the anti-inflammatory capacity of COO.
32394442	11	49	theme	comprehensive	1733:1745	arg1	utilization					1747:1757	comprehensive utilization	1733:1757	comprehensive utilization	1733:1757	This may provide a scientific basis for the comprehensive utilization and development of COO and related functional foods.
32394442	10	50	theme	main	1638:1641	arg1	ingredient					1654:1663	the main functional ingredient	1634:1663	the main functional ingredient	1634:1663	Our research confirms the anti-inflammatory potential of COO and highlights that the main functional ingredient is polyphenol extracts.
32394442	10	50	theme	main	1638:1641	arg1	extracts					1679:1686	polyphenol extracts	1668:1686	polyphenol extracts	1668:1686	Our research confirms the anti-inflammatory potential of COO and highlights that the main functional ingredient is polyphenol extracts.
32394442	0	51	theme	Oil	59:61	arg1	Composition					26:36	Phytochemical Composition	12:36	Phytochemical Composition of Camellia oleifera Oil	12:61	Analysis of Phytochemical Composition of Camellia oleifera Oil and Evaluation of its Anti-Inflammatory Effect in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages.
32394442	9	52	theme	system	1531:1536	arg1	feasibility					1511:1521	the feasibility	1507:1521	the feasibility of this system	1507:1536	In addition, an oil-in-water (O/W) emulsion-based system was also developed to deliver the liposoluble COO into the cells, and the feasibility of this system was confirmed.
32394442	2	53	from	research	445:452	arg1	capacity					479:486	the anti-inflammatory capacity	457:486	the anti-inflammatory capacity of COO	457:493	However, there has been a lack of relevant biological research on the anti-inflammatory capacity of COO, and the specific bioactive lipid phytochemicals contributing to the anti-inflammatory effect need further research.
32394442	0	54	theme	oleifera	50:57	arg1	Oil					59:61	Camellia oleifera Oil	41:61	Camellia oleifera Oil	41:61	Analysis of Phytochemical Composition of Camellia oleifera Oil and Evaluation of its Anti-Inflammatory Effect in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages.
32394442	11	55	theme	foods	1805:1809	arg1	development					1763:1773	development	1763:1773	development	1763:1773	This may provide a scientific basis for the comprehensive utilization and development of COO and related functional foods.
32394442	11	55	theme	foods	1805:1809	arg1	utilization					1747:1757	comprehensive utilization	1733:1757	comprehensive utilization	1733:1757	This may provide a scientific basis for the comprehensive utilization and development of COO and related functional foods.
32394442	2	56	theme	lipid	523:527	arg1	phytochemicals					529:542	the specific bioactive lipid phytochemicals	500:542	the specific bioactive lipid phytochemicals contributing to the anti-inflammatory effect	500:587	However, there has been a lack of relevant biological research on the anti-inflammatory capacity of COO, and the specific bioactive lipid phytochemicals contributing to the anti-inflammatory effect need further research.
32394442	3	57	theme	RAW	631:633	arg1	macrophages					641:651	RAW 264.7 macrophages	631:651	the RAW 264.7 macrophages model	627:657	In this study, the RAW 264.7 macrophages model was used to investigate the anti-inflammatory capacity of COO.
32394442	9	58	theme	liposoluble	1471:1481	arg1	COO					1483:1485	the liposoluble COO	1467:1485	the liposoluble COO into the cells	1467:1500	In addition, an oil-in-water (O/W) emulsion-based system was also developed to deliver the liposoluble COO into the cells, and the feasibility of this system was confirmed.
32394442	10	59	theme	COO	1610:1612	arg1	potential					1597:1605	the anti-inflammatory potential	1575:1605	the anti-inflammatory potential of COO	1575:1612	Our research confirms the anti-inflammatory potential of COO and highlights that the main functional ingredient is polyphenol extracts.
32394442	2	60	theme	bioactive	513:521	arg1	phytochemicals					529:542	the specific bioactive lipid phytochemicals	500:542	the specific bioactive lipid phytochemicals contributing to the anti-inflammatory effect	500:587	However, there has been a lack of relevant biological research on the anti-inflammatory capacity of COO, and the specific bioactive lipid phytochemicals contributing to the anti-inflammatory effect need further research.
32394442	11	61	theme	scientific	1708:1717	arg1	basis					1719:1723	a scientific basis	1706:1723	a scientific basis for the comprehensive utilization and development of COO and related functional foods	1706:1809	This may provide a scientific basis for the comprehensive utilization and development of COO and related functional foods.
32394442	5	62	theme	Nos2	890:893	arg1	suppression					875:885	the suppression	871:885	the suppression of Nos2 and Cox-2 expression	871:914	secretion via the suppression of Nos2 and Cox-2 expression.
32394442	2	63	theme	specific	504:511	arg1	phytochemicals					529:542	the specific bioactive lipid phytochemicals	500:542	the specific bioactive lipid phytochemicals contributing to the anti-inflammatory effect	500:587	However, there has been a lack of relevant biological research on the anti-inflammatory capacity of COO, and the specific bioactive lipid phytochemicals contributing to the anti-inflammatory effect need further research.
32394442	4	64	theme	33-200 μg/mL	743:754	arg1	COO					756:758	33-200 μg/mL COO	743:758	33-200 μg/mL COO	743:758	Our data showed that 33-200 μg/mL COO markedly inhibited the lipopolysaccharide lipopolysaccharide (LPS)-stimulated nitric oxide (NO.)
32394442	8	65	theme	polyphenol	1298:1307	arg1	extracts					1309:1316	the polyphenol extracts	1294:1316	the polyphenol extracts of COO	1294:1323	It was interestingly demonstrated that the polyphenol extracts of COO play a vital role in its anti-inflammatory properties.
32394442	1	66	theme	common	218:223	arg1	oil					232:234	a common edible oil	216:234	not only a common edible oil but also a traditional remedy widely applied to ameliorate a variety of illnesses associated with inflammation, such as mouth ulcers, thrush, eczema, etc	207:388	In China, Camellia oleifera oil (COO) is not only a common edible oil but also a traditional remedy widely applied to ameliorate a variety of illnesses associated with inflammation, such as mouth ulcers, thrush, eczema, etc.
32394442	1	66	theme	common	218:223	arg1	oil					194:196	Camellia oleifera oil	176:196	Camellia oleifera oil (COO)	176:202	In China, Camellia oleifera oil (COO) is not only a common edible oil but also a traditional remedy widely applied to ameliorate a variety of illnesses associated with inflammation, such as mouth ulcers, thrush, eczema, etc.
32394442	2	67	from	capacity	479:486	arg1	lack					417:420	a lack	415:420	a lack of relevant biological research on the anti-inflammatory capacity of COO	415:493	However, there has been a lack of relevant biological research on the anti-inflammatory capacity of COO, and the specific bioactive lipid phytochemicals contributing to the anti-inflammatory effect need further research.
32394442	9	68	theme	emulsion-based	1415:1428	arg1	system					1430:1435	an oil-in-water (O/W) emulsion-based system	1393:1435	an oil-in-water (O/W) emulsion-based system	1393:1435	In addition, an oil-in-water (O/W) emulsion-based system was also developed to deliver the liposoluble COO into the cells, and the feasibility of this system was confirmed.
32394442	1	69	theme	edible	225:230	arg1	oil					232:234	a common edible oil	216:234	not only a common edible oil but also a traditional remedy widely applied to ameliorate a variety of illnesses associated with inflammation, such as mouth ulcers, thrush, eczema, etc	207:388	In China, Camellia oleifera oil (COO) is not only a common edible oil but also a traditional remedy widely applied to ameliorate a variety of illnesses associated with inflammation, such as mouth ulcers, thrush, eczema, etc.
32394442	1	69	theme	edible	225:230	arg1	oil					194:196	Camellia oleifera oil	176:196	Camellia oleifera oil (COO)	176:202	In China, Camellia oleifera oil (COO) is not only a common edible oil but also a traditional remedy widely applied to ameliorate a variety of illnesses associated with inflammation, such as mouth ulcers, thrush, eczema, etc.
32394442	2	70	theme	research	445:452	arg1	lack					417:420	a lack	415:420	a lack of relevant biological research on the anti-inflammatory capacity of COO	415:493	However, there has been a lack of relevant biological research on the anti-inflammatory capacity of COO, and the specific bioactive lipid phytochemicals contributing to the anti-inflammatory effect need further research.
32394442	1	71	theme	illnesses	308:316	arg1	illnesses					308:316	illnesses	308:316	illnesses	308:316	In China, Camellia oleifera oil (COO) is not only a common edible oil but also a traditional remedy widely applied to ameliorate a variety of illnesses associated with inflammation, such as mouth ulcers, thrush, eczema, etc.
32394442	1	71	theme	illnesses	308:316	arg1	variety					297:303	a variety	295:303	a variety of illnesses associated with inflammation, such as mouth ulcers, thrush, eczema, etc	295:388	In China, Camellia oleifera oil (COO) is not only a common edible oil but also a traditional remedy widely applied to ameliorate a variety of illnesses associated with inflammation, such as mouth ulcers, thrush, eczema, etc.
32394442	1	71	theme	illnesses	308:316	arg1	ulcers					362:367	mouth ulcers	356:367	mouth ulcers	356:367	In China, Camellia oleifera oil (COO) is not only a common edible oil but also a traditional remedy widely applied to ameliorate a variety of illnesses associated with inflammation, such as mouth ulcers, thrush, eczema, etc.
32394442	7	72	theme	chemical	1232:1239	arg1	compositions					1241:1252	their chemical compositions	1226:1252	their chemical compositions	1226:1252	To further explore the correlation between the anti-inflammatory effects and the lipid phytochemicals in COO, 10 samples were collected and screened for their chemical compositions.
32394442	6	73	theme	strong	976:981	arg1	effect					995:1000	a strong suppressive effect	974:1000	a strong suppressive effect on the expression of proinflammatory cytokines such as Tnf-α and Il-6	974:1070	The enzyme immunoassay confirmed that COO also exhibited a strong suppressive effect on the expression of proinflammatory cytokines such as Tnf-α and Il-6.
31971478	8	0	theme	surface	1031:1037	arg1	compression					1039:1049	the surface compression	1027:1049	the surface compression of the film	1027:1061	The results of SEM showed that the PPE particles were uniformly distributed in the film matrix and the MOE slightly reduced the surface compression of the film.
31971478	4	1	theme	loss	645:648	arg1	amount					626:631	the amount	622:631	the amount of moisture loss	622:648	The results showed that the addition of PPE significantly reduced the amount of moisture loss.
31971478	4	1	theme	loss	645:648	arg1	loss					645:648	moisture loss	636:648	moisture loss	636:648	The results showed that the addition of PPE significantly reduced the amount of moisture loss.
31971478	7	2	theme	microbial	807:815	arg1	properties					817:826	microbial properties	807:826	microbial properties	807:826	In terms of microbial properties, the antimicrobial effect of MOE on B. cereus was greater than on E. coli.
31971478	8	3	theme	SEM	918:920	arg1	results					907:913	The results	903:913	The results of SEM	903:920	The results of SEM showed that the PPE particles were uniformly distributed in the film matrix and the MOE slightly reduced the surface compression of the film.
31971478	4	4	theme	moisture	636:643	arg1	loss					645:648	moisture loss	636:648	moisture loss	636:648	The results showed that the addition of PPE significantly reduced the amount of moisture loss.
31971478	12	5	theme	naked	1506:1510	arg1	eye					1512:1514	the naked eye	1502:1514	the naked eye	1502:1514	The colour change of the film was visible with the naked eye and can be used to estimate the expiry date of the cheese.
31971478	0	6	theme	cheeses	100:106	arg1	spoilage					108:115	cream cheeses spoilage	94:115	cream cheeses spoilage	94:115	Smart film based on chitosan/Melissa officinalis essences/ pomegranate peel extract to detect cream cheeses spoilage.
31971478	10	7	theme	cheese	1287:1292	arg1	pH					1271:1272	the pH	1267:1272	the pH of the cream cheese	1267:1292	It was observed that with increasing storage time and storage temperature, with regard to the change in the pH of the cream cheese, samples become acidic.
31971478	11	8	theme	pH	1378:1379	arg1	changes					1381:1387	pH changes	1378:1387	pH changes	1378:1387	The PPE contains anthocyanin pigments that are sensitive to pH changes so the film colour was changed (from blue to red) during storage.
31971478	11	9	dep	changed	1412:1418	arg1	red					1434:1436	red	1434:1436	red	1434:1436	The PPE contains anthocyanin pigments that are sensitive to pH changes so the film colour was changed (from blue to red) during storage.
31971478	7	10	from	effect	847:852	arg1	cereus					867:872	B. cereus	864:872	B. cereus	864:872	In terms of microbial properties, the antimicrobial effect of MOE on B. cereus was greater than on E. coli.
31971478	0	11	theme	cream	94:98	arg1	spoilage					108:115	cream cheeses spoilage	94:115	cream cheeses spoilage	94:115	Smart film based on chitosan/Melissa officinalis essences/ pomegranate peel extract to detect cream cheeses spoilage.
31971478	10	12	theme	cream	1281:1285	arg1	cheese					1287:1292	the cream cheese	1277:1292	the cream cheese	1277:1292	It was observed that with increasing storage time and storage temperature, with regard to the change in the pH of the cream cheese, samples become acidic.
31971478	9	13	theme	CS/PPE/MOE	1068:1077	arg1	film					1079:1082	The CS/PPE/MOE film	1064:1082	The CS/PPE/MOE film	1064:1082	The CS/PPE/MOE film was used for smart packaging of cream cheese and detection of cheese spoilage.
31971478	3	14	theme	microbial	490:498	arg1	properties					500:509	microbial properties	490:509	microbial properties	490:509	The physicochemical characteristics of the film, including moisture, water vapour permeability (WVP), antioxidant activity, microbial properties, FT-IR/SEM/XRED spectrum, etc. were studied.
31971478	3	14	theme	microbial	490:498	arg1	moisture					425:432	moisture	425:432	moisture	425:432	The physicochemical characteristics of the film, including moisture, water vapour permeability (WVP), antioxidant activity, microbial properties, FT-IR/SEM/XRED spectrum, etc. were studied.
31971478	10	15	from	change	1257:1262	arg1	pH					1271:1272	the pH	1267:1272	the pH of the cream cheese	1267:1292	It was observed that with increasing storage time and storage temperature, with regard to the change in the pH of the cream cheese, samples become acidic.
31971478	8	16	theme	film	1058:1061	arg1	compression					1039:1049	the surface compression	1027:1049	the surface compression of the film	1027:1061	The results of SEM showed that the PPE particles were uniformly distributed in the film matrix and the MOE slightly reduced the surface compression of the film.
31971478	3	17	theme	vapour	441:446	arg1	WVP					462:464	WVP	462:464	WVP	462:464	The physicochemical characteristics of the film, including moisture, water vapour permeability (WVP), antioxidant activity, microbial properties, FT-IR/SEM/XRED spectrum, etc. were studied.
31971478	3	17	theme	vapour	441:446	arg1	permeability					448:459	water vapour permeability	435:459	water vapour permeability (WVP)	435:465	The physicochemical characteristics of the film, including moisture, water vapour permeability (WVP), antioxidant activity, microbial properties, FT-IR/SEM/XRED spectrum, etc. were studied.
31971478	3	17	theme	vapour	441:446	arg1	moisture					425:432	moisture	425:432	moisture	425:432	The physicochemical characteristics of the film, including moisture, water vapour permeability (WVP), antioxidant activity, microbial properties, FT-IR/SEM/XRED spectrum, etc. were studied.
31971478	3	18	theme	antioxidant	468:478	arg1	activity					480:487	antioxidant activity	468:487	antioxidant activity	468:487	The physicochemical characteristics of the film, including moisture, water vapour permeability (WVP), antioxidant activity, microbial properties, FT-IR/SEM/XRED spectrum, etc. were studied.
31971478	3	18	theme	antioxidant	468:478	arg1	moisture					425:432	moisture	425:432	moisture	425:432	The physicochemical characteristics of the film, including moisture, water vapour permeability (WVP), antioxidant activity, microbial properties, FT-IR/SEM/XRED spectrum, etc. were studied.
31971478	7	19	theme	properties	817:826	arg1	terms					798:802	terms	798:802	terms of microbial properties	798:826	In terms of microbial properties, the antimicrobial effect of MOE on B. cereus was greater than on E. coli.
31971478	1	20	theme	/pomegranate	173:184	arg1	extract					191:197	Chitosan (CS)/pomegranate peel extract	160:197	Chitosan (CS)/pomegranate peel extract (PPE)	160:203	Antibacterial/biodegradable film based on Chitosan (CS)/pomegranate peel extract (PPE) and Melissa officinalis Essences (MOE) was prepared.
31971478	1	20	theme	/pomegranate	173:184	arg1	PPE					200:202	PPE	200:202	PPE	200:202	Antibacterial/biodegradable film based on Chitosan (CS)/pomegranate peel extract (PPE) and Melissa officinalis Essences (MOE) was prepared.
31971478	5	21	theme	PPE	729:731	arg1	addition					717:724	the addition	713:724	the addition of PPE	713:731	The WVP increased with the addition of MOE and decreased with the addition of PPE.
31971478	8	22	theme	PPE	938:940	arg1	particles					942:950	the PPE particles	934:950	the PPE particles	934:950	The results of SEM showed that the PPE particles were uniformly distributed in the film matrix and the MOE slightly reduced the surface compression of the film.
31971478	7	23	theme	MOE	857:859	arg1	effect					847:852	the antimicrobial effect	829:852	the antimicrobial effect of MOE on B. cereus	829:872	In terms of microbial properties, the antimicrobial effect of MOE on B. cereus was greater than on E. coli.
31971478	7	23	theme	MOE	857:859	arg1	greater					878:884	greater	878:884	greater	878:884	In terms of microbial properties, the antimicrobial effect of MOE on B. cereus was greater than on E. coli.
31971478	1	24	theme	peel	186:189	arg1	extract					191:197	Chitosan (CS)/pomegranate peel extract	160:197	Chitosan (CS)/pomegranate peel extract (PPE)	160:203	Antibacterial/biodegradable film based on Chitosan (CS)/pomegranate peel extract (PPE) and Melissa officinalis Essences (MOE) was prepared.
31971478	1	24	theme	peel	186:189	arg1	PPE					200:202	PPE	200:202	PPE	200:202	Antibacterial/biodegradable film based on Chitosan (CS)/pomegranate peel extract (PPE) and Melissa officinalis Essences (MOE) was prepared.
31971478	2	25	theme	MOE	290:292	arg1	effects					271:277	the effects	267:277	the effects of PPE and MOE on the film characteristics	267:320	To study the effects of PPE and MOE on the film characteristics, a central composite design (CCD) was used.
31971478	12	26	theme	film	1480:1483	arg1	visible					1489:1495	visible	1489:1495	visible	1489:1495	The colour change of the film was visible with the naked eye and can be used to estimate the expiry date of the cheese.
31971478	12	26	theme	film	1480:1483	arg1	change					1466:1471	The colour change	1455:1471	The colour change of the film	1455:1483	The colour change of the film was visible with the naked eye and can be used to estimate the expiry date of the cheese.
31971478	3	27	theme	physicochemical	370:384	arg1	characteristics					386:400	The physicochemical characteristics	366:400	The physicochemical characteristics	366:400	The physicochemical characteristics of the film, including moisture, water vapour permeability (WVP), antioxidant activity, microbial properties, FT-IR/SEM/XRED spectrum, etc. were studied.
31971478	3	27	theme	physicochemical	370:384	arg1	moisture					425:432	moisture	425:432	moisture	425:432	The physicochemical characteristics of the film, including moisture, water vapour permeability (WVP), antioxidant activity, microbial properties, FT-IR/SEM/XRED spectrum, etc. were studied.
31971478	0	28	theme	Smart	0:4	arg1	film					6:9	Smart film	0:9	Smart film	0:9	Smart film based on chitosan/Melissa officinalis essences/ pomegranate peel extract to detect cream cheeses spoilage.
31971478	11	29	theme	film	1396:1399	arg1	colour					1401:1406	the film colour	1392:1406	the film colour	1392:1406	The PPE contains anthocyanin pigments that are sensitive to pH changes so the film colour was changed (from blue to red) during storage.
31971478	11	30	dep	red	1434:1436	arg1	to					1431:1432	to	1431:1432	to	1431:1432	The PPE contains anthocyanin pigments that are sensitive to pH changes so the film colour was changed (from blue to red) during storage.
31971478	9	31	theme	smart	1097:1101	arg1	packaging					1103:1111	smart packaging	1097:1111	smart packaging of cream cheese and detection of cheese spoilage	1097:1160	The CS/PPE/MOE film was used for smart packaging of cream cheese and detection of cheese spoilage.
31971478	2	32	theme	PPE	282:284	arg1	effects					271:277	the effects	267:277	the effects of PPE and MOE on the film characteristics	267:320	To study the effects of PPE and MOE on the film characteristics, a central composite design (CCD) was used.
31971478	9	33	used	used	1088:1091	arg2	film					1079:1082	The CS/PPE/MOE film	1064:1082	The CS/PPE/MOE film	1064:1082	The CS/PPE/MOE film was used for smart packaging of cream cheese and detection of cheese spoilage.
31971478	12	34	with	visible	1489:1495	arg1	eye					1512:1514	the naked eye	1502:1514	the naked eye	1502:1514	The colour change of the film was visible with the naked eye and can be used to estimate the expiry date of the cheese.
31971478	12	35	used	used	1527:1530	arg2	visible					1489:1495	visible	1489:1495	visible	1489:1495	The colour change of the film was visible with the naked eye and can be used to estimate the expiry date of the cheese.
31971478	12	35	used	used	1527:1530	arg2	change					1466:1471	The colour change	1455:1471	The colour change of the film	1455:1483	The colour change of the film was visible with the naked eye and can be used to estimate the expiry date of the cheese.
31971478	1	36	theme	Chitosan	160:167	arg1	extract					191:197	Chitosan (CS)/pomegranate peel extract	160:197	Chitosan (CS)/pomegranate peel extract (PPE)	160:203	Antibacterial/biodegradable film based on Chitosan (CS)/pomegranate peel extract (PPE) and Melissa officinalis Essences (MOE) was prepared.
31971478	1	36	theme	Chitosan	160:167	arg1	PPE					200:202	PPE	200:202	PPE	200:202	Antibacterial/biodegradable film based on Chitosan (CS)/pomegranate peel extract (PPE) and Melissa officinalis Essences (MOE) was prepared.
31971478	0	37	theme	officinalis	37:47	arg1	extract					76:82	chitosan/Melissa officinalis essences/ pomegranate peel extract	20:82	chitosan/Melissa officinalis essences/ pomegranate peel extract	20:82	Smart film based on chitosan/Melissa officinalis essences/ pomegranate peel extract to detect cream cheeses spoilage.
31971478	8	38	theme	film	986:989	arg1	matrix					991:996	the film matrix	982:996	the film matrix	982:996	The results of SEM showed that the PPE particles were uniformly distributed in the film matrix and the MOE slightly reduced the surface compression of the film.
31971478	6	39	theme	films	788:792	arg1	activity					772:779	the antioxidant activity	756:779	the antioxidant activity of the films	756:792	MOE and PPE increased the antioxidant activity of the films.
31971478	12	40	theme	colour	1459:1464	arg1	visible					1489:1495	visible	1489:1495	visible	1489:1495	The colour change of the film was visible with the naked eye and can be used to estimate the expiry date of the cheese.
31971478	12	40	theme	colour	1459:1464	arg1	change					1466:1471	The colour change	1455:1471	The colour change of the film	1455:1483	The colour change of the film was visible with the naked eye and can be used to estimate the expiry date of the cheese.
31971478	3	41	theme	film	409:412	arg1	characteristics					386:400	The physicochemical characteristics	366:400	The physicochemical characteristics	366:400	The physicochemical characteristics of the film, including moisture, water vapour permeability (WVP), antioxidant activity, microbial properties, FT-IR/SEM/XRED spectrum, etc. were studied.
31971478	3	41	theme	film	409:412	arg1	moisture					425:432	moisture	425:432	moisture	425:432	The physicochemical characteristics of the film, including moisture, water vapour permeability (WVP), antioxidant activity, microbial properties, FT-IR/SEM/XRED spectrum, etc. were studied.
31971478	0	42	theme	chitosan/Melissa	20:35	arg1	extract					76:82	chitosan/Melissa officinalis essences/ pomegranate peel extract	20:82	chitosan/Melissa officinalis essences/ pomegranate peel extract	20:82	Smart film based on chitosan/Melissa officinalis essences/ pomegranate peel extract to detect cream cheeses spoilage.
31971478	9	43	theme	cream	1116:1120	arg1	packaging					1103:1111	smart packaging	1097:1111	smart packaging of cream cheese and detection of cheese spoilage	1097:1160	The CS/PPE/MOE film was used for smart packaging of cream cheese and detection of cheese spoilage.
31971478	9	44	dep	cream	1116:1120	arg1	cheese					1122:1127	cheese	1122:1127	cheese	1122:1127	The CS/PPE/MOE film was used for smart packaging of cream cheese and detection of cheese spoilage.
31971478	10	45	theme	storage	1217:1223	arg1	temperature					1225:1235	storage temperature	1217:1235	storage temperature	1217:1235	It was observed that with increasing storage time and storage temperature, with regard to the change in the pH of the cream cheese, samples become acidic.
31971478	1	46	theme	Melissa	209:215	arg1	MOE					239:241	MOE	239:241	MOE	239:241	Antibacterial/biodegradable film based on Chitosan (CS)/pomegranate peel extract (PPE) and Melissa officinalis Essences (MOE) was prepared.
31971478	1	46	theme	Melissa	209:215	arg1	Essences					229:236	Melissa officinalis Essences	209:236	Melissa officinalis Essences (MOE)	209:242	Antibacterial/biodegradable film based on Chitosan (CS)/pomegranate peel extract (PPE) and Melissa officinalis Essences (MOE) was prepared.
31971478	0	47	theme	pomegranate	59:69	arg1	extract					76:82	chitosan/Melissa officinalis essences/ pomegranate peel extract	20:82	chitosan/Melissa officinalis essences/ pomegranate peel extract	20:82	Smart film based on chitosan/Melissa officinalis essences/ pomegranate peel extract to detect cream cheeses spoilage.
31971478	2	48	used	used	360:363	arg2	design					343:348	a central composite design	323:348	a central composite design (CCD)	323:354	To study the effects of PPE and MOE on the film characteristics, a central composite design (CCD) was used.
31971478	2	48	used	used	360:363	arg2	CCD					351:353	CCD	351:353	CCD	351:353	To study the effects of PPE and MOE on the film characteristics, a central composite design (CCD) was used.
31971478	1	49	theme	Antibacterial/biodegradable	118:144	arg1	film					146:149	Antibacterial/biodegradable film	118:149	Antibacterial/biodegradable film based on Chitosan (CS)/pomegranate peel extract (PPE) and Melissa officinalis Essences (MOE)	118:242	Antibacterial/biodegradable film based on Chitosan (CS)/pomegranate peel extract (PPE) and Melissa officinalis Essences (MOE) was prepared.
31971478	0	50	theme	essences/	49:57	arg1	extract					76:82	chitosan/Melissa officinalis essences/ pomegranate peel extract	20:82	chitosan/Melissa officinalis essences/ pomegranate peel extract	20:82	Smart film based on chitosan/Melissa officinalis essences/ pomegranate peel extract to detect cream cheeses spoilage.
31971478	11	51	contain	contains	1326:1333	arg1	PPE					1322:1324	The PPE	1318:1324	The PPE	1318:1324	The PPE contains anthocyanin pigments that are sensitive to pH changes so the film colour was changed (from blue to red) during storage.
31971478	11	51	contain	contains	1326:1333	arg2	pigments					1347:1354	anthocyanin pigments	1335:1354	anthocyanin pigments that are sensitive to pH changes so the film colour was changed (from blue to red) during storage	1335:1452	The PPE contains anthocyanin pigments that are sensitive to pH changes so the film colour was changed (from blue to red) during storage.
31971478	9	52	theme	detection	1133:1141	arg1	packaging					1103:1111	smart packaging	1097:1111	smart packaging of cream cheese and detection of cheese spoilage	1097:1160	The CS/PPE/MOE film was used for smart packaging of cream cheese and detection of cheese spoilage.
31971478	2	53	theme	film	301:304	arg1	characteristics					306:320	the film characteristics	297:320	the film characteristics	297:320	To study the effects of PPE and MOE on the film characteristics, a central composite design (CCD) was used.
31971478	11	54	theme	anthocyanin	1335:1345	arg1	pigments					1347:1354	anthocyanin pigments	1335:1354	anthocyanin pigments that are sensitive to pH changes so the film colour was changed (from blue to red) during storage	1335:1452	The PPE contains anthocyanin pigments that are sensitive to pH changes so the film colour was changed (from blue to red) during storage.
31971478	3	55	theme	FT-IR/SEM/XRED	512:525	arg1	moisture					425:432	moisture	425:432	moisture	425:432	The physicochemical characteristics of the film, including moisture, water vapour permeability (WVP), antioxidant activity, microbial properties, FT-IR/SEM/XRED spectrum, etc. were studied.
31971478	3	55	theme	FT-IR/SEM/XRED	512:525	arg1	spectrum					527:534	FT-IR/SEM/XRED spectrum	512:534	FT-IR/SEM/XRED spectrum	512:534	The physicochemical characteristics of the film, including moisture, water vapour permeability (WVP), antioxidant activity, microbial properties, FT-IR/SEM/XRED spectrum, etc. were studied.
31971478	6	56	theme	antioxidant	760:770	arg1	activity					772:779	the antioxidant activity	756:779	the antioxidant activity of the films	756:792	MOE and PPE increased the antioxidant activity of the films.
31971478	10	57	theme	storage	1200:1206	arg1	time					1208:1211	storage time	1200:1211	storage time	1200:1211	It was observed that with increasing storage time and storage temperature, with regard to the change in the pH of the cream cheese, samples become acidic.
31971478	7	58	theme	antimicrobial	833:845	arg1	effect					847:852	the antimicrobial effect	829:852	the antimicrobial effect of MOE on B. cereus	829:872	In terms of microbial properties, the antimicrobial effect of MOE on B. cereus was greater than on E. coli.
31971478	7	58	theme	antimicrobial	833:845	arg1	greater					878:884	greater	878:884	greater	878:884	In terms of microbial properties, the antimicrobial effect of MOE on B. cereus was greater than on E. coli.
31971478	1	59	theme	officinalis	217:227	arg1	MOE					239:241	MOE	239:241	MOE	239:241	Antibacterial/biodegradable film based on Chitosan (CS)/pomegranate peel extract (PPE) and Melissa officinalis Essences (MOE) was prepared.
31971478	1	59	theme	officinalis	217:227	arg1	Essences					229:236	Melissa officinalis Essences	209:236	Melissa officinalis Essences (MOE)	209:242	Antibacterial/biodegradable film based on Chitosan (CS)/pomegranate peel extract (PPE) and Melissa officinalis Essences (MOE) was prepared.
31971478	3	60	theme	water	435:439	arg1	WVP					462:464	WVP	462:464	WVP	462:464	The physicochemical characteristics of the film, including moisture, water vapour permeability (WVP), antioxidant activity, microbial properties, FT-IR/SEM/XRED spectrum, etc. were studied.
31971478	3	60	theme	water	435:439	arg1	permeability					448:459	water vapour permeability	435:459	water vapour permeability (WVP)	435:465	The physicochemical characteristics of the film, including moisture, water vapour permeability (WVP), antioxidant activity, microbial properties, FT-IR/SEM/XRED spectrum, etc. were studied.
31971478	3	60	theme	water	435:439	arg1	moisture					425:432	moisture	425:432	moisture	425:432	The physicochemical characteristics of the film, including moisture, water vapour permeability (WVP), antioxidant activity, microbial properties, FT-IR/SEM/XRED spectrum, etc. were studied.
31971478	2	61	from	effects	271:277	arg1	characteristics					306:320	the film characteristics	297:320	the film characteristics	297:320	To study the effects of PPE and MOE on the film characteristics, a central composite design (CCD) was used.
31971478	5	62	theme	MOE	690:692	arg1	addition					678:685	the addition	674:685	the addition of MOE	674:692	The WVP increased with the addition of MOE and decreased with the addition of PPE.
31971478	9	63	theme	cheese	1146:1151	arg1	spoilage					1153:1160	cheese spoilage	1146:1160	cheese spoilage	1146:1160	The CS/PPE/MOE film was used for smart packaging of cream cheese and detection of cheese spoilage.
31971478	12	64	theme	expiry	1548:1553	arg1	date					1555:1558	the expiry date	1544:1558	the expiry date of the cheese	1544:1572	The colour change of the film was visible with the naked eye and can be used to estimate the expiry date of the cheese.
31971478	12	65	theme	cheese	1567:1572	arg1	date					1555:1558	the expiry date	1544:1558	the expiry date of the cheese	1544:1572	The colour change of the film was visible with the naked eye and can be used to estimate the expiry date of the cheese.
31971478	2	66	theme	composite	333:341	arg1	CCD					351:353	CCD	351:353	CCD	351:353	To study the effects of PPE and MOE on the film characteristics, a central composite design (CCD) was used.
31971478	2	66	theme	composite	333:341	arg1	design					343:348	a central composite design	323:348	a central composite design (CCD)	323:354	To study the effects of PPE and MOE on the film characteristics, a central composite design (CCD) was used.
31971478	4	67	theme	PPE	596:598	arg1	addition					584:591	the addition	580:591	the addition of PPE	580:598	The results showed that the addition of PPE significantly reduced the amount of moisture loss.
31971478	0	68	theme	peel	71:74	arg1	extract					76:82	chitosan/Melissa officinalis essences/ pomegranate peel extract	20:82	chitosan/Melissa officinalis essences/ pomegranate peel extract	20:82	Smart film based on chitosan/Melissa officinalis essences/ pomegranate peel extract to detect cream cheeses spoilage.
31971478	9	69	theme	spoilage	1153:1160	arg1	cream					1116:1120	cream cheese and detection	1116:1141	cream	1116:1120	The CS/PPE/MOE film was used for smart packaging of cream cheese and detection of cheese spoilage.
31971478	9	69	theme	spoilage	1153:1160	arg1	detection					1133:1141	detection	1133:1141	detection	1133:1141	The CS/PPE/MOE film was used for smart packaging of cream cheese and detection of cheese spoilage.
31971478	2	70	theme	central	325:331	arg1	CCD					351:353	CCD	351:353	CCD	351:353	To study the effects of PPE and MOE on the film characteristics, a central composite design (CCD) was used.
31971478	2	70	theme	central	325:331	arg1	design					343:348	a central composite design	323:348	a central composite design (CCD)	323:354	To study the effects of PPE and MOE on the film characteristics, a central composite design (CCD) was used.
34553599	6	0	theme	pulse	1284:1288	arg1	pressure					1272:1279	a subtle pressure	1263:1279	a subtle pressure of pulse	1263:1288	With the high sensitivity in a wide range, the sensor is capable of detecting multiple human and animal activities in real time, ranging from the large pressure of joint activities to a subtle pressure of pulse.
34553599	3	1	theme	sensor	525:530	arg1	layer					512:516	the force-sensitive layer	492:516	the force-sensitive layer of the sensor	492:530	In the force-sensitive layer of the sensor, MXene and CS are fully attached to the PU sponge to ensure that the composite sponge has remarkable conductivity and washability.
34553599	4	2	from	spacer	713:718	arg1	Benefiting					663:672	Benefiting	663:672	Benefiting from the highly resistive PVA-nanowire spacer, the initial current of the sensor	663:753	Benefiting from the highly resistive PVA-nanowire spacer, the initial current of the sensor is reduced significantly so that the sensor exhibits extremely high sensitivity (84.9 kPa-1 for the less than 5 kPa region and 140.6 kPa-1 for the 5-22 kPa region).
34553599	1	3	theme	nice	240:243	arg1	interaction					259:269	a nice human-machine interaction	238:269	a nice human-machine interaction	238:269	Wearable pressure sensors are highly desirable for monitoring human health and realizing a nice human-machine interaction.
34553599	6	4	theme	joint	1243:1247	arg1	activities					1249:1258	joint activities	1243:1258	joint activities	1243:1258	With the high sensitivity in a wide range, the sensor is capable of detecting multiple human and animal activities in real time, ranging from the large pressure of joint activities to a subtle pressure of pulse.
34553599	1	5	theme	Wearable	149:156	arg1	sensors					167:173	Wearable pressure sensors	149:173	Wearable pressure sensors	149:173	Wearable pressure sensors are highly desirable for monitoring human health and realizing a nice human-machine interaction.
34553599	8	6	theme	pressure	1427:1434	arg1	sensor					1436:1441	such a multifunctional pressure sensor	1404:1441	such a multifunctional pressure sensor	1404:1441	Overall, such a multifunctional pressure sensor can supply a new platform for the design and development of wearable health-monitoring equipment and an efficient human-machine interface.
34553599	1	7	theme	human-machine	245:257	arg1	interaction					259:269	a nice human-machine interaction	238:269	a nice human-machine interaction	238:269	Wearable pressure sensors are highly desirable for monitoring human health and realizing a nice human-machine interaction.
34553599	0	8	theme	3D	83:84	arg1	Network					93:99	a 3D Sponge Network	81:99	a 3D Sponge Network for Real-Time Monitoring Human Body Activities	81:146	Wearable, Washable, and Highly Sensitive Piezoresistive Pressure Sensor Based on a 3D Sponge Network for Real-Time Monitoring Human Body Activities.
34553599	5	9	theme	response	963:970	arg1	time					972:975	an excellent fast response time	945:975	an excellent fast response time of 200 ms	945:985	Moreover, the sensor has an excellent fast response time of 200 ms and a short recovery time of 30 ms, as well as non-attenuating durability over 5000 cycles.
34553599	6	10	theme	real	1197:1200	arg1	time					1202:1205	real time	1197:1205	real time	1197:1205	With the high sensitivity in a wide range, the sensor is capable of detecting multiple human and animal activities in real time, ranging from the large pressure of joint activities to a subtle pressure of pulse.
34553599	2	11	theme	chitosan/MXene/polyurethane-sponge/polyvinyl	282:325	arg1	sensor					378:383	a chitosan/MXene/polyurethane-sponge/polyvinyl alcohol (CS/MXene/PU sponge/PVA)-based 3D pressure sensor	280:383	a chitosan/MXene/polyurethane-sponge/polyvinyl alcohol (CS/MXene/PU sponge/PVA)-based 3D pressure sensor	280:383	Herein, a chitosan/MXene/polyurethane-sponge/polyvinyl alcohol (CS/MXene/PU sponge/PVA)-based 3D pressure sensor is developed to simultaneously achieve wearability, washability, and high sensitivity in a wide region.
34553599	3	12	theme	PU	572:573	arg1	sponge					575:580	the PU sponge	568:580	the PU sponge	568:580	In the force-sensitive layer of the sensor, MXene and CS are fully attached to the PU sponge to ensure that the composite sponge has remarkable conductivity and washability.
34553599	7	13	theme	pressure	1372:1379	arg1	distribution					1381:1392	pressure distribution	1372:1392	pressure distribution	1372:1392	Furthermore, the sensor also demonstrates the potential application in measuring pressure distribution.
34553599	8	14	theme	interface	1571:1579	arg1	development					1488:1498	development	1488:1498	development	1488:1498	Overall, such a multifunctional pressure sensor can supply a new platform for the design and development of wearable health-monitoring equipment and an efficient human-machine interface.
34553599	8	14	theme	interface	1571:1579	arg1	design					1477:1482	design	1477:1482	design	1477:1482	Overall, such a multifunctional pressure sensor can supply a new platform for the design and development of wearable health-monitoring equipment and an efficient human-machine interface.
34553599	6	15	theme	wide	1110:1113	arg1	range					1115:1119	a wide range	1108:1119	a wide range	1108:1119	With the high sensitivity in a wide range, the sensor is capable of detecting multiple human and animal activities in real time, ranging from the large pressure of joint activities to a subtle pressure of pulse.
34553599	6	16	theme	activities	1249:1258	arg1	pressure					1231:1238	the large pressure	1221:1238	the large pressure of joint activities	1221:1258	With the high sensitivity in a wide range, the sensor is capable of detecting multiple human and animal activities in real time, ranging from the large pressure of joint activities to a subtle pressure of pulse.
34553599	4	17	theme	initial	725:731	arg1	spacer					713:718	the highly resistive PVA-nanowire spacer	679:718	the highly resistive PVA-nanowire spacer	679:718	Benefiting from the highly resistive PVA-nanowire spacer, the initial current of the sensor is reduced significantly so that the sensor exhibits extremely high sensitivity (84.9 kPa-1 for the less than 5 kPa region and 140.6 kPa-1 for the 5-22 kPa region).
34553599	4	17	theme	initial	725:731	arg1	current					733:739	the initial current	721:739	the initial current of the sensor	721:753	Benefiting from the highly resistive PVA-nanowire spacer, the initial current of the sensor is reduced significantly so that the sensor exhibits extremely high sensitivity (84.9 kPa-1 for the less than 5 kPa region and 140.6 kPa-1 for the 5-22 kPa region).
34553599	4	18	theme	sensor	748:753	arg1	spacer					713:718	the highly resistive PVA-nanowire spacer	679:718	the highly resistive PVA-nanowire spacer	679:718	Benefiting from the highly resistive PVA-nanowire spacer, the initial current of the sensor is reduced significantly so that the sensor exhibits extremely high sensitivity (84.9 kPa-1 for the less than 5 kPa region and 140.6 kPa-1 for the 5-22 kPa region).
34553599	4	18	theme	sensor	748:753	arg1	current					733:739	the initial current	721:739	the initial current of the sensor	721:753	Benefiting from the highly resistive PVA-nanowire spacer, the initial current of the sensor is reduced significantly so that the sensor exhibits extremely high sensitivity (84.9 kPa-1 for the less than 5 kPa region and 140.6 kPa-1 for the 5-22 kPa region).
34553599	1	19	theme	pressure	158:165	arg1	sensors					167:173	Wearable pressure sensors	149:173	Wearable pressure sensors	149:173	Wearable pressure sensors are highly desirable for monitoring human health and realizing a nice human-machine interaction.
34553599	0	20	theme	Sponge	86:91	arg1	Network					93:99	a 3D Sponge Network	81:99	a 3D Sponge Network for Real-Time Monitoring Human Body Activities	81:146	Wearable, Washable, and Highly Sensitive Piezoresistive Pressure Sensor Based on a 3D Sponge Network for Real-Time Monitoring Human Body Activities.
34553599	5	21	theme	non-attenuating	1034:1048	arg1	durability					1050:1059	non-attenuating durability	1034:1059	non-attenuating durability	1034:1059	Moreover, the sensor has an excellent fast response time of 200 ms and a short recovery time of 30 ms, as well as non-attenuating durability over 5000 cycles.
34553599	6	22	theme	animal	1176:1181	arg1	activities					1183:1192	multiple human and animal activities	1157:1192	multiple human and animal activities	1157:1192	With the high sensitivity in a wide range, the sensor is capable of detecting multiple human and animal activities in real time, ranging from the large pressure of joint activities to a subtle pressure of pulse.
34553599	4	23	dep	sensitivity	823:833	arg1	kPa-1					888:892	140.6 kPa-1	882:892	140.6 kPa-1 for the 5-22 kPa region	882:916	Benefiting from the highly resistive PVA-nanowire spacer, the initial current of the sensor is reduced significantly so that the sensor exhibits extremely high sensitivity (84.9 kPa-1 for the less than 5 kPa region and 140.6 kPa-1 for the 5-22 kPa region).
34553599	4	23	dep	sensitivity	823:833	arg1	kPa-1					841:845	84.9 kPa-1	836:845	84.9 kPa-1 for the less than 5 kPa region	836:876	Benefiting from the highly resistive PVA-nanowire spacer, the initial current of the sensor is reduced significantly so that the sensor exhibits extremely high sensitivity (84.9 kPa-1 for the less than 5 kPa region and 140.6 kPa-1 for the 5-22 kPa region).
34553599	5	24	theme	excellent	948:956	arg1	time					972:975	an excellent fast response time	945:975	an excellent fast response time of 200 ms	945:985	Moreover, the sensor has an excellent fast response time of 200 ms and a short recovery time of 30 ms, as well as non-attenuating durability over 5000 cycles.
34553599	5	25	contain	has	941:943	arg2	time					972:975	an excellent fast response time	945:975	an excellent fast response time of 200 ms	945:985	Moreover, the sensor has an excellent fast response time of 200 ms and a short recovery time of 30 ms, as well as non-attenuating durability over 5000 cycles.
34553599	5	25	contain	has	941:943	arg2	durability					1050:1059	non-attenuating durability	1034:1059	non-attenuating durability	1034:1059	Moreover, the sensor has an excellent fast response time of 200 ms and a short recovery time of 30 ms, as well as non-attenuating durability over 5000 cycles.
34553599	5	25	contain	has	941:943	arg1	sensor					934:939	the sensor	930:939	the sensor	930:939	Moreover, the sensor has an excellent fast response time of 200 ms and a short recovery time of 30 ms, as well as non-attenuating durability over 5000 cycles.
34553599	5	25	contain	has	941:943	arg2	time					1008:1011	a short recovery time	991:1011	a short recovery time of 30 ms	991:1020	Moreover, the sensor has an excellent fast response time of 200 ms and a short recovery time of 30 ms, as well as non-attenuating durability over 5000 cycles.
34553599	2	26	theme	wide	476:479	arg1	region					481:486	a wide region	474:486	a wide region	474:486	Herein, a chitosan/MXene/polyurethane-sponge/polyvinyl alcohol (CS/MXene/PU sponge/PVA)-based 3D pressure sensor is developed to simultaneously achieve wearability, washability, and high sensitivity in a wide region.
34553599	2	27	theme	sponge/PVA	348:357	arg1	sensor					378:383	a chitosan/MXene/polyurethane-sponge/polyvinyl alcohol (CS/MXene/PU sponge/PVA)-based 3D pressure sensor	280:383	a chitosan/MXene/polyurethane-sponge/polyvinyl alcohol (CS/MXene/PU sponge/PVA)-based 3D pressure sensor	280:383	Herein, a chitosan/MXene/polyurethane-sponge/polyvinyl alcohol (CS/MXene/PU sponge/PVA)-based 3D pressure sensor is developed to simultaneously achieve wearability, washability, and high sensitivity in a wide region.
34553599	0	28	theme	Wearable	0:7	arg1	Sensor					65:70	Wearable, Washable, and Highly Sensitive Piezoresistive Pressure Sensor	0:70	Wearable, Washable, and Highly Sensitive Piezoresistive Pressure Sensor	0:70	Wearable, Washable, and Highly Sensitive Piezoresistive Pressure Sensor Based on a 3D Sponge Network for Real-Time Monitoring Human Body Activities.
34553599	7	29	theme	potential	1337:1345	arg1	application					1347:1357	the potential application	1333:1357	the potential application	1333:1357	Furthermore, the sensor also demonstrates the potential application in measuring pressure distribution.
34553599	6	30	theme	human	1166:1170	arg1	activities					1183:1192	multiple human and animal activities	1157:1192	multiple human and animal activities	1157:1192	With the high sensitivity in a wide range, the sensor is capable of detecting multiple human and animal activities in real time, ranging from the large pressure of joint activities to a subtle pressure of pulse.
34553599	2	31	theme	CS/MXene/PU	336:346	arg1	sensor					378:383	a chitosan/MXene/polyurethane-sponge/polyvinyl alcohol (CS/MXene/PU sponge/PVA)-based 3D pressure sensor	280:383	a chitosan/MXene/polyurethane-sponge/polyvinyl alcohol (CS/MXene/PU sponge/PVA)-based 3D pressure sensor	280:383	Herein, a chitosan/MXene/polyurethane-sponge/polyvinyl alcohol (CS/MXene/PU sponge/PVA)-based 3D pressure sensor is developed to simultaneously achieve wearability, washability, and high sensitivity in a wide region.
34553599	3	32	attach	attached	556:563	arg2	CS					543:544	CS	543:544	CS	543:544	In the force-sensitive layer of the sensor, MXene and CS are fully attached to the PU sponge to ensure that the composite sponge has remarkable conductivity and washability.
34553599	3	32	attach	attached	556:563	arg2	MXene					533:537	MXene	533:537	MXene	533:537	In the force-sensitive layer of the sensor, MXene and CS are fully attached to the PU sponge to ensure that the composite sponge has remarkable conductivity and washability.
34553599	3	32	attach	attached	556:563	arg3	layer					512:516	the force-sensitive layer	492:516	the force-sensitive layer of the sensor	492:530	In the force-sensitive layer of the sensor, MXene and CS are fully attached to the PU sponge to ensure that the composite sponge has remarkable conductivity and washability.
34553599	3	32	attach	attached	556:563	arg1	sponge					575:580	the PU sponge	568:580	the PU sponge	568:580	In the force-sensitive layer of the sensor, MXene and CS are fully attached to the PU sponge to ensure that the composite sponge has remarkable conductivity and washability.
34553599	8	33	theme	human-machine	1557:1569	arg1	interface					1571:1579	an efficient human-machine interface	1544:1579	an efficient human-machine interface	1544:1579	Overall, such a multifunctional pressure sensor can supply a new platform for the design and development of wearable health-monitoring equipment and an efficient human-machine interface.
34553599	4	34	theme	high	818:821	arg1	sensitivity					823:833	extremely high sensitivity	808:833	extremely high sensitivity (84.9 kPa-1 for the less than 5 kPa region and 140.6 kPa-1 for the 5-22 kPa region)	808:917	Benefiting from the highly resistive PVA-nanowire spacer, the initial current of the sensor is reduced significantly so that the sensor exhibits extremely high sensitivity (84.9 kPa-1 for the less than 5 kPa region and 140.6 kPa-1 for the 5-22 kPa region).
34553599	0	35	theme	Real-Time	105:113	arg1	Monitoring					115:124	Real-Time Monitoring	105:124	Real-Time Monitoring Human Body Activities	105:146	Wearable, Washable, and Highly Sensitive Piezoresistive Pressure Sensor Based on a 3D Sponge Network for Real-Time Monitoring Human Body Activities.
34553599	2	36	theme	high	454:457	arg1	sensitivity					459:469	high sensitivity	454:469	high sensitivity	454:469	Herein, a chitosan/MXene/polyurethane-sponge/polyvinyl alcohol (CS/MXene/PU sponge/PVA)-based 3D pressure sensor is developed to simultaneously achieve wearability, washability, and high sensitivity in a wide region.
34553599	0	37	theme	Washable	10:17	arg1	Sensor					65:70	Wearable, Washable, and Highly Sensitive Piezoresistive Pressure Sensor	0:70	Wearable, Washable, and Highly Sensitive Piezoresistive Pressure Sensor	0:70	Wearable, Washable, and Highly Sensitive Piezoresistive Pressure Sensor Based on a 3D Sponge Network for Real-Time Monitoring Human Body Activities.
34553599	4	38	theme	5-22	902:905	arg1	region					911:916	the 5-22 kPa region	898:916	the 5-22 kPa region	898:916	Benefiting from the highly resistive PVA-nanowire spacer, the initial current of the sensor is reduced significantly so that the sensor exhibits extremely high sensitivity (84.9 kPa-1 for the less than 5 kPa region and 140.6 kPa-1 for the 5-22 kPa region).
34553599	2	39	theme	alcohol	327:333	arg1	sensor					378:383	a chitosan/MXene/polyurethane-sponge/polyvinyl alcohol (CS/MXene/PU sponge/PVA)-based 3D pressure sensor	280:383	a chitosan/MXene/polyurethane-sponge/polyvinyl alcohol (CS/MXene/PU sponge/PVA)-based 3D pressure sensor	280:383	Herein, a chitosan/MXene/polyurethane-sponge/polyvinyl alcohol (CS/MXene/PU sponge/PVA)-based 3D pressure sensor is developed to simultaneously achieve wearability, washability, and high sensitivity in a wide region.
34553599	6	40	from	sensitivity	1093:1103	arg1	range					1115:1119	a wide range	1108:1119	a wide range	1108:1119	With the high sensitivity in a wide range, the sensor is capable of detecting multiple human and animal activities in real time, ranging from the large pressure of joint activities to a subtle pressure of pulse.
34553599	6	41	theme	high	1088:1091	arg1	sensitivity					1093:1103	the high sensitivity	1084:1103	the high sensitivity in a wide range	1084:1119	With the high sensitivity in a wide range, the sensor is capable of detecting multiple human and animal activities in real time, ranging from the large pressure of joint activities to a subtle pressure of pulse.
34553599	6	42	with	capable	1136:1142	arg1	sensitivity					1093:1103	the high sensitivity	1084:1103	the high sensitivity in a wide range	1084:1119	With the high sensitivity in a wide range, the sensor is capable of detecting multiple human and animal activities in real time, ranging from the large pressure of joint activities to a subtle pressure of pulse.
34553599	0	43	theme	Human	126:130	arg1	Activities					137:146	Real-Time Monitoring Human Body Activities	105:146	Real-Time Monitoring Human Body Activities	105:146	Wearable, Washable, and Highly Sensitive Piezoresistive Pressure Sensor Based on a 3D Sponge Network for Real-Time Monitoring Human Body Activities.
34553599	5	44	theme	ms	1019:1020	arg1	time					972:975	an excellent fast response time	945:975	an excellent fast response time of 200 ms	945:985	Moreover, the sensor has an excellent fast response time of 200 ms and a short recovery time of 30 ms, as well as non-attenuating durability over 5000 cycles.
34553599	5	44	theme	ms	1019:1020	arg1	time					1008:1011	a short recovery time	991:1011	a short recovery time of 30 ms	991:1020	Moreover, the sensor has an excellent fast response time of 200 ms and a short recovery time of 30 ms, as well as non-attenuating durability over 5000 cycles.
34553599	5	44	theme	ms	1019:1020	arg1	durability					1050:1059	non-attenuating durability	1034:1059	non-attenuating durability	1034:1059	Moreover, the sensor has an excellent fast response time of 200 ms and a short recovery time of 30 ms, as well as non-attenuating durability over 5000 cycles.
34553599	6	45	theme	multiple	1157:1164	arg1	activities					1183:1192	multiple human and animal activities	1157:1192	multiple human and animal activities	1157:1192	With the high sensitivity in a wide range, the sensor is capable of detecting multiple human and animal activities in real time, ranging from the large pressure of joint activities to a subtle pressure of pulse.
34553599	4	46	dep	region	871:876	arg1	kPa					867:869	kPa	867:869	kPa	867:869	Benefiting from the highly resistive PVA-nanowire spacer, the initial current of the sensor is reduced significantly so that the sensor exhibits extremely high sensitivity (84.9 kPa-1 for the less than 5 kPa region and 140.6 kPa-1 for the 5-22 kPa region).
34553599	0	47	theme	Monitoring	115:124	arg1	Activities					137:146	Real-Time Monitoring Human Body Activities	105:146	Real-Time Monitoring Human Body Activities	105:146	Wearable, Washable, and Highly Sensitive Piezoresistive Pressure Sensor Based on a 3D Sponge Network for Real-Time Monitoring Human Body Activities.
34553599	5	48	theme	ms	984:985	arg1	time					972:975	an excellent fast response time	945:975	an excellent fast response time of 200 ms	945:985	Moreover, the sensor has an excellent fast response time of 200 ms and a short recovery time of 30 ms, as well as non-attenuating durability over 5000 cycles.
34553599	5	48	theme	ms	984:985	arg1	time					1008:1011	a short recovery time	991:1011	a short recovery time of 30 ms	991:1020	Moreover, the sensor has an excellent fast response time of 200 ms and a short recovery time of 30 ms, as well as non-attenuating durability over 5000 cycles.
34553599	5	48	theme	ms	984:985	arg1	durability					1050:1059	non-attenuating durability	1034:1059	non-attenuating durability	1034:1059	Moreover, the sensor has an excellent fast response time of 200 ms and a short recovery time of 30 ms, as well as non-attenuating durability over 5000 cycles.
34553599	1	49	theme	human	211:215	arg1	health					217:222	human health	211:222	human health	211:222	Wearable pressure sensors are highly desirable for monitoring human health and realizing a nice human-machine interaction.
34553599	0	50	theme	Piezoresistive	41:54	arg1	Sensor					65:70	Wearable, Washable, and Highly Sensitive Piezoresistive Pressure Sensor	0:70	Wearable, Washable, and Highly Sensitive Piezoresistive Pressure Sensor	0:70	Wearable, Washable, and Highly Sensitive Piezoresistive Pressure Sensor Based on a 3D Sponge Network for Real-Time Monitoring Human Body Activities.
34553599	6	51	theme	large	1225:1229	arg1	pressure					1231:1238	the large pressure	1221:1238	the large pressure of joint activities	1221:1258	With the high sensitivity in a wide range, the sensor is capable of detecting multiple human and animal activities in real time, ranging from the large pressure of joint activities to a subtle pressure of pulse.
34553599	8	52	theme	new	1456:1458	arg1	platform					1460:1467	a new platform	1454:1467	a new platform for the design and development of wearable health-monitoring equipment and an efficient human-machine interface	1454:1579	Overall, such a multifunctional pressure sensor can supply a new platform for the design and development of wearable health-monitoring equipment and an efficient human-machine interface.
34553599	2	53	theme	pressure	369:376	arg1	sensor					378:383	a chitosan/MXene/polyurethane-sponge/polyvinyl alcohol (CS/MXene/PU sponge/PVA)-based 3D pressure sensor	280:383	a chitosan/MXene/polyurethane-sponge/polyvinyl alcohol (CS/MXene/PU sponge/PVA)-based 3D pressure sensor	280:383	Herein, a chitosan/MXene/polyurethane-sponge/polyvinyl alcohol (CS/MXene/PU sponge/PVA)-based 3D pressure sensor is developed to simultaneously achieve wearability, washability, and high sensitivity in a wide region.
34553599	0	54	theme	Sensitive	31:39	arg1	Sensor					65:70	Wearable, Washable, and Highly Sensitive Piezoresistive Pressure Sensor	0:70	Wearable, Washable, and Highly Sensitive Piezoresistive Pressure Sensor	0:70	Wearable, Washable, and Highly Sensitive Piezoresistive Pressure Sensor Based on a 3D Sponge Network for Real-Time Monitoring Human Body Activities.
34553599	8	55	theme	wearable	1503:1510	arg1	equipment					1530:1538	wearable health-monitoring equipment	1503:1538	wearable health-monitoring equipment	1503:1538	Overall, such a multifunctional pressure sensor can supply a new platform for the design and development of wearable health-monitoring equipment and an efficient human-machine interface.
34553599	0	56	theme	Body	132:135	arg1	Activities					137:146	Real-Time Monitoring Human Body Activities	105:146	Real-Time Monitoring Human Body Activities	105:146	Wearable, Washable, and Highly Sensitive Piezoresistive Pressure Sensor Based on a 3D Sponge Network for Real-Time Monitoring Human Body Activities.
34553599	5	57	theme	short	993:997	arg1	time					1008:1011	a short recovery time	991:1011	a short recovery time of 30 ms	991:1020	Moreover, the sensor has an excellent fast response time of 200 ms and a short recovery time of 30 ms, as well as non-attenuating durability over 5000 cycles.
34553599	2	58	theme	3D	366:367	arg1	sensor					378:383	a chitosan/MXene/polyurethane-sponge/polyvinyl alcohol (CS/MXene/PU sponge/PVA)-based 3D pressure sensor	280:383	a chitosan/MXene/polyurethane-sponge/polyvinyl alcohol (CS/MXene/PU sponge/PVA)-based 3D pressure sensor	280:383	Herein, a chitosan/MXene/polyurethane-sponge/polyvinyl alcohol (CS/MXene/PU sponge/PVA)-based 3D pressure sensor is developed to simultaneously achieve wearability, washability, and high sensitivity in a wide region.
34553599	8	59	theme	efficient	1547:1555	arg1	interface					1571:1579	an efficient human-machine interface	1544:1579	an efficient human-machine interface	1544:1579	Overall, such a multifunctional pressure sensor can supply a new platform for the design and development of wearable health-monitoring equipment and an efficient human-machine interface.
34553599	5	60	theme	recovery	999:1006	arg1	time					1008:1011	a short recovery time	991:1011	a short recovery time of 30 ms	991:1020	Moreover, the sensor has an excellent fast response time of 200 ms and a short recovery time of 30 ms, as well as non-attenuating durability over 5000 cycles.
34553599	5	61	theme	fast	958:961	arg1	time					972:975	an excellent fast response time	945:975	an excellent fast response time of 200 ms	945:985	Moreover, the sensor has an excellent fast response time of 200 ms and a short recovery time of 30 ms, as well as non-attenuating durability over 5000 cycles.
34553599	2	62	theme	-based	359:364	arg1	sensor					378:383	a chitosan/MXene/polyurethane-sponge/polyvinyl alcohol (CS/MXene/PU sponge/PVA)-based 3D pressure sensor	280:383	a chitosan/MXene/polyurethane-sponge/polyvinyl alcohol (CS/MXene/PU sponge/PVA)-based 3D pressure sensor	280:383	Herein, a chitosan/MXene/polyurethane-sponge/polyvinyl alcohol (CS/MXene/PU sponge/PVA)-based 3D pressure sensor is developed to simultaneously achieve wearability, washability, and high sensitivity in a wide region.
34553599	8	63	theme	equipment	1530:1538	arg1	development					1488:1498	development	1488:1498	development	1488:1498	Overall, such a multifunctional pressure sensor can supply a new platform for the design and development of wearable health-monitoring equipment and an efficient human-machine interface.
34553599	8	63	theme	equipment	1530:1538	arg1	design					1477:1482	design	1477:1482	design	1477:1482	Overall, such a multifunctional pressure sensor can supply a new platform for the design and development of wearable health-monitoring equipment and an efficient human-machine interface.
34553599	8	64	dep	design	1477:1482	arg1	the					1473:1475	the	1473:1475	the	1473:1475	Overall, such a multifunctional pressure sensor can supply a new platform for the design and development of wearable health-monitoring equipment and an efficient human-machine interface.
34553599	3	65	theme	remarkable	622:631	arg1	conductivity					633:644	remarkable conductivity	622:644	remarkable conductivity	622:644	In the force-sensitive layer of the sensor, MXene and CS are fully attached to the PU sponge to ensure that the composite sponge has remarkable conductivity and washability.
34553599	6	66	theme	subtle	1265:1270	arg1	pressure					1272:1279	a subtle pressure	1263:1279	a subtle pressure of pulse	1263:1288	With the high sensitivity in a wide range, the sensor is capable of detecting multiple human and animal activities in real time, ranging from the large pressure of joint activities to a subtle pressure of pulse.
34553599	3	67	theme	force-sensitive	496:510	arg1	layer					512:516	the force-sensitive layer	492:516	the force-sensitive layer of the sensor	492:530	In the force-sensitive layer of the sensor, MXene and CS are fully attached to the PU sponge to ensure that the composite sponge has remarkable conductivity and washability.
34553599	4	68	theme	kPa	907:909	arg1	region					911:916	the 5-22 kPa region	898:916	the 5-22 kPa region	898:916	Benefiting from the highly resistive PVA-nanowire spacer, the initial current of the sensor is reduced significantly so that the sensor exhibits extremely high sensitivity (84.9 kPa-1 for the less than 5 kPa region and 140.6 kPa-1 for the 5-22 kPa region).
34553599	3	69	theme	composite	601:609	arg1	sponge					611:616	the composite sponge	597:616	the composite sponge	597:616	In the force-sensitive layer of the sensor, MXene and CS are fully attached to the PU sponge to ensure that the composite sponge has remarkable conductivity and washability.
34553599	4	70	theme	PVA-nanowire	700:711	arg1	spacer					713:718	the highly resistive PVA-nanowire spacer	679:718	the highly resistive PVA-nanowire spacer	679:718	Benefiting from the highly resistive PVA-nanowire spacer, the initial current of the sensor is reduced significantly so that the sensor exhibits extremely high sensitivity (84.9 kPa-1 for the less than 5 kPa region and 140.6 kPa-1 for the 5-22 kPa region).
34553599	4	70	theme	PVA-nanowire	700:711	arg1	current					733:739	the initial current	721:739	the initial current of the sensor	721:753	Benefiting from the highly resistive PVA-nanowire spacer, the initial current of the sensor is reduced significantly so that the sensor exhibits extremely high sensitivity (84.9 kPa-1 for the less than 5 kPa region and 140.6 kPa-1 for the 5-22 kPa region).
34553599	0	71	theme	Pressure	56:63	arg1	Sensor					65:70	Wearable, Washable, and Highly Sensitive Piezoresistive Pressure Sensor	0:70	Wearable, Washable, and Highly Sensitive Piezoresistive Pressure Sensor	0:70	Wearable, Washable, and Highly Sensitive Piezoresistive Pressure Sensor Based on a 3D Sponge Network for Real-Time Monitoring Human Body Activities.
34553599	8	72	theme	multifunctional	1411:1425	arg1	sensor					1436:1441	such a multifunctional pressure sensor	1404:1441	such a multifunctional pressure sensor	1404:1441	Overall, such a multifunctional pressure sensor can supply a new platform for the design and development of wearable health-monitoring equipment and an efficient human-machine interface.
34553599	8	73	theme	health-monitoring	1512:1528	arg1	equipment					1530:1538	wearable health-monitoring equipment	1503:1538	wearable health-monitoring equipment	1503:1538	Overall, such a multifunctional pressure sensor can supply a new platform for the design and development of wearable health-monitoring equipment and an efficient human-machine interface.
34553599	4	74	dep	kPa	867:869	arg1	5					865:865	5	865:865	5	865:865	Benefiting from the highly resistive PVA-nanowire spacer, the initial current of the sensor is reduced significantly so that the sensor exhibits extremely high sensitivity (84.9 kPa-1 for the less than 5 kPa region and 140.6 kPa-1 for the 5-22 kPa region).
34553599	4	75	theme	resistive	690:698	arg1	spacer					713:718	the highly resistive PVA-nanowire spacer	679:718	the highly resistive PVA-nanowire spacer	679:718	Benefiting from the highly resistive PVA-nanowire spacer, the initial current of the sensor is reduced significantly so that the sensor exhibits extremely high sensitivity (84.9 kPa-1 for the less than 5 kPa region and 140.6 kPa-1 for the 5-22 kPa region).
34553599	4	75	theme	resistive	690:698	arg1	current					733:739	the initial current	721:739	the initial current of the sensor	721:753	Benefiting from the highly resistive PVA-nanowire spacer, the initial current of the sensor is reduced significantly so that the sensor exhibits extremely high sensitivity (84.9 kPa-1 for the less than 5 kPa region and 140.6 kPa-1 for the 5-22 kPa region).
34553599	3	76	contain	has	618:620	arg1	sponge					611:616	the composite sponge	597:616	the composite sponge	597:616	In the force-sensitive layer of the sensor, MXene and CS are fully attached to the PU sponge to ensure that the composite sponge has remarkable conductivity and washability.
34553599	3	76	contain	has	618:620	arg2	washability					650:660	washability	650:660	washability	650:660	In the force-sensitive layer of the sensor, MXene and CS are fully attached to the PU sponge to ensure that the composite sponge has remarkable conductivity and washability.
34553599	3	76	contain	has	618:620	arg2	conductivity					633:644	remarkable conductivity	622:644	remarkable conductivity	622:644	In the force-sensitive layer of the sensor, MXene and CS are fully attached to the PU sponge to ensure that the composite sponge has remarkable conductivity and washability.
32758600	4	0	theme	structure	704:712	arg1	features					714:721	the surface structure features	692:721	the surface structure features	692:721	The addition of AOB had not induced notable change on the surface structure features.
32758600	3	1	theme	solutions	508:516	arg1	viscosity					482:490	the viscosity	478:490	the viscosity of film forming solutions	478:516	The physical properties, including the viscosity of film forming solutions, the water vapor permeability, mechanical properties, thermodynamics and transparence of CMC-Gel films were determined.
32758600	1	2	theme	CMC-Gel	262:268	arg1	mixture					271:277	carboxymethyl cellulose-gelatin (CMC-Gel) mixture	229:277	carboxymethyl cellulose-gelatin (CMC-Gel) mixture using casting method	229:298	In this study functional film enrichment with antioxidant of bamboo leaves (AOB) was prepared based on carboxymethyl cellulose-gelatin (CMC-Gel) mixture using casting method.
32758600	7	3	theme	AOB	1222:1224	arg1	concentration					1205:1217	the concentration	1201:1217	the concentration of AOB	1201:1224	The water vapor permeation resistance and elongation at break of the films significantly decreased when the concentration of AOB exceeded 0.2%.
32758600	6	4	dep	779.2 ± 26.2 mPa·s	999:1016	arg1	higher					1057:1062	higher	1057:1062	higher	1057:1062	Viscosity and glass transition temperature of the film with 0.2% AOB were 779.2 ± 26.2 mPa·s and 83.24 °C, respectively, which were higher than those of all other samples.
32758600	4	5	theme	surface	696:702	arg1	features					714:721	the surface structure features	692:721	the surface structure features	692:721	The addition of AOB had not induced notable change on the surface structure features.
32758600	9	6	theme	mechanical	1478:1487	arg1	force					1489:1493	mechanical force	1478:1493	mechanical force	1478:1493	Consequently, the antioxidant films utilized for food packaging could protect food quality from the destruction of heat, mechanical force, light, and oxidative free radicals.
32758600	7	7	theme	permeation	1113:1122	arg1	resistance					1124:1133	permeation resistance	1113:1133	permeation resistance	1113:1133	The water vapor permeation resistance and elongation at break of the films significantly decreased when the concentration of AOB exceeded 0.2%.
32758600	7	8	from	break	1153:1157	arg1	vapor					1107:1111	The water vapor permeation resistance and elongation	1097:1148	vapor	1107:1111	The water vapor permeation resistance and elongation at break of the films significantly decreased when the concentration of AOB exceeded 0.2%.
32758600	7	8	from	break	1153:1157	arg1	elongation					1139:1148	elongation	1139:1148	elongation	1139:1148	The water vapor permeation resistance and elongation at break of the films significantly decreased when the concentration of AOB exceeded 0.2%.
32758600	1	9	with	enrichment	156:165	arg1	antioxidant					172:182	antioxidant	172:182	antioxidant	172:182	In this study functional film enrichment with antioxidant of bamboo leaves (AOB) was prepared based on carboxymethyl cellulose-gelatin (CMC-Gel) mixture using casting method.
32758600	6	10	with	film	975:978	arg1	AOB					990:992	0.2% AOB	985:992	0.2% AOB	985:992	Viscosity and glass transition temperature of the film with 0.2% AOB were 779.2 ± 26.2 mPa·s and 83.24 °C, respectively, which were higher than those of all other samples.
32758600	9	11	theme	food	1406:1409	arg1	packaging					1411:1419	food packaging	1406:1419	food packaging	1406:1419	Consequently, the antioxidant films utilized for food packaging could protect food quality from the destruction of heat, mechanical force, light, and oxidative free radicals.
32758600	0	12	theme	bamboo	111:116	arg1	antioxidant					96:106	antioxidant	96:106	antioxidant of bamboo leaves	96:123	Structural and physical properties of carboxymethyl cellulose/gelatin films functionalized with antioxidant of bamboo leaves.
32758600	9	13	theme	light	1496:1500	arg1	destruction					1457:1467	the destruction	1453:1467	the destruction of heat, mechanical force, light, and oxidative free radicals	1453:1529	Consequently, the antioxidant films utilized for food packaging could protect food quality from the destruction of heat, mechanical force, light, and oxidative free radicals.
32758600	8	14	theme	AOB	1344:1346	arg1	content					1348:1354	AOB content	1344:1354	AOB content	1344:1354	The light barrier properties and scavenging rate of DPPH radicals were enhanced with the increasing of AOB content.
32758600	3	15	theme	mechanical	549:558	arg1	viscosity					482:490	the viscosity	478:490	the viscosity of film forming solutions	478:516	The physical properties, including the viscosity of film forming solutions, the water vapor permeability, mechanical properties, thermodynamics and transparence of CMC-Gel films were determined.
32758600	3	15	theme	mechanical	549:558	arg1	properties					456:465	The physical properties	443:465	The physical properties	443:465	The physical properties, including the viscosity of film forming solutions, the water vapor permeability, mechanical properties, thermodynamics and transparence of CMC-Gel films were determined.
32758600	3	15	theme	mechanical	549:558	arg1	properties					560:569	mechanical properties	549:569	mechanical properties	549:569	The physical properties, including the viscosity of film forming solutions, the water vapor permeability, mechanical properties, thermodynamics and transparence of CMC-Gel films were determined.
32758600	5	16	theme	composite	860:868	arg1	film					870:873	CMC-Gel composite film	852:873	CMC-Gel composite film	852:873	The good compatibility among AOB, CMC and gelatin was illustrated by XRD for that the crystallization disappeared of gelatin in CMC-Gel composite film and no diffraction peaks in functionalized films.
32758600	9	17	theme	heat	1472:1475	arg1	destruction					1457:1467	the destruction	1453:1467	the destruction of heat, mechanical force, light, and oxidative free radicals	1453:1529	Consequently, the antioxidant films utilized for food packaging could protect food quality from the destruction of heat, mechanical force, light, and oxidative free radicals.
32758600	3	18	theme	film	495:498	arg1	solutions					508:516	film forming solutions	495:516	film forming solutions	495:516	The physical properties, including the viscosity of film forming solutions, the water vapor permeability, mechanical properties, thermodynamics and transparence of CMC-Gel films were determined.
32758600	5	19	from	peaks	894:898	arg1	films					918:922	functionalized films	903:922	functionalized films	903:922	The good compatibility among AOB, CMC and gelatin was illustrated by XRD for that the crystallization disappeared of gelatin in CMC-Gel composite film and no diffraction peaks in functionalized films.
32758600	2	20	dep	transform	381:389	arg1	infrared					391:398	infrared	391:398	transform infrared spectrometer	381:411	Microstructures of the films were performed on atomic force microscope, Fourier transform infrared spectrometer, and X-ray diffraction (XRD).
32758600	1	21	theme	bamboo	187:192	arg1	enrichment					156:165	functional film enrichment	140:165	functional film enrichment with antioxidant of bamboo leaves (AOB)	140:205	In this study functional film enrichment with antioxidant of bamboo leaves (AOB) was prepared based on carboxymethyl cellulose-gelatin (CMC-Gel) mixture using casting method.
32758600	6	22	theme	%	988:988	arg1	AOB					990:992	0.2% AOB	985:992	0.2% AOB	985:992	Viscosity and glass transition temperature of the film with 0.2% AOB were 779.2 ± 26.2 mPa·s and 83.24 °C, respectively, which were higher than those of all other samples.
32758600	7	23	dep	vapor	1107:1111	arg1	resistance					1124:1133	permeation resistance	1113:1133	permeation resistance	1113:1133	The water vapor permeation resistance and elongation at break of the films significantly decreased when the concentration of AOB exceeded 0.2%.
32758600	0	24	dep	bamboo	111:116	arg1	leaves					118:123	leaves	118:123	leaves	118:123	Structural and physical properties of carboxymethyl cellulose/gelatin films functionalized with antioxidant of bamboo leaves.
32758600	6	25	theme	other	1082:1086	arg1	samples					1088:1094	all other samples	1078:1094	all other samples	1078:1094	Viscosity and glass transition temperature of the film with 0.2% AOB were 779.2 ± 26.2 mPa·s and 83.24 °C, respectively, which were higher than those of all other samples.
32758600	3	26	theme	physical	447:454	arg1	properties					560:569	mechanical properties	549:569	mechanical properties	549:569	The physical properties, including the viscosity of film forming solutions, the water vapor permeability, mechanical properties, thermodynamics and transparence of CMC-Gel films were determined.
32758600	3	26	theme	physical	447:454	arg1	viscosity					482:490	the viscosity	478:490	the viscosity of film forming solutions	478:516	The physical properties, including the viscosity of film forming solutions, the water vapor permeability, mechanical properties, thermodynamics and transparence of CMC-Gel films were determined.
32758600	3	26	theme	physical	447:454	arg1	thermodynamics					572:585	thermodynamics	572:585	thermodynamics	572:585	The physical properties, including the viscosity of film forming solutions, the water vapor permeability, mechanical properties, thermodynamics and transparence of CMC-Gel films were determined.
32758600	3	26	theme	physical	447:454	arg1	permeability					535:546	the water vapor permeability	519:546	the water vapor permeability	519:546	The physical properties, including the viscosity of film forming solutions, the water vapor permeability, mechanical properties, thermodynamics and transparence of CMC-Gel films were determined.
32758600	3	26	theme	physical	447:454	arg1	properties					456:465	The physical properties	443:465	The physical properties	443:465	The physical properties, including the viscosity of film forming solutions, the water vapor permeability, mechanical properties, thermodynamics and transparence of CMC-Gel films were determined.
32758600	3	26	theme	physical	447:454	arg1	transparence					591:602	transparence	591:602	transparence	591:602	The physical properties, including the viscosity of film forming solutions, the water vapor permeability, mechanical properties, thermodynamics and transparence of CMC-Gel films were determined.
32758600	7	27	theme	films	1166:1170	arg1	vapor					1107:1111	The water vapor permeation resistance and elongation	1097:1148	vapor	1107:1111	The water vapor permeation resistance and elongation at break of the films significantly decreased when the concentration of AOB exceeded 0.2%.
32758600	7	27	theme	films	1166:1170	arg1	elongation					1139:1148	elongation	1139:1148	elongation	1139:1148	The water vapor permeation resistance and elongation at break of the films significantly decreased when the concentration of AOB exceeded 0.2%.
32758600	9	28	theme	oxidative	1507:1515	arg1	radicals					1522:1529	oxidative free radicals	1507:1529	oxidative free radicals	1507:1529	Consequently, the antioxidant films utilized for food packaging could protect food quality from the destruction of heat, mechanical force, light, and oxidative free radicals.
32758600	6	29	theme	0.2	985:987	arg1	%					988:988	%	988:988	%	988:988	Viscosity and glass transition temperature of the film with 0.2% AOB were 779.2 ± 26.2 mPa·s and 83.24 °C, respectively, which were higher than those of all other samples.
32758600	8	30	theme	light	1245:1249	arg1	properties					1259:1268	The light barrier properties	1241:1268	The light barrier properties	1241:1268	The light barrier properties and scavenging rate of DPPH radicals were enhanced with the increasing of AOB content.
32758600	0	31	theme	Structural	0:9	arg1	properties					24:33	Structural and physical properties	0:33	Structural and physical properties of carboxymethyl cellulose/gelatin films	0:74	Structural and physical properties of carboxymethyl cellulose/gelatin films functionalized with antioxidant of bamboo leaves.
32758600	1	32	theme	casting	285:291	arg1	method					293:298	casting method	285:298	casting method	285:298	In this study functional film enrichment with antioxidant of bamboo leaves (AOB) was prepared based on carboxymethyl cellulose-gelatin (CMC-Gel) mixture using casting method.
32758600	2	33	theme	films	324:328	arg1	Microstructures					301:315	Microstructures	301:315	Microstructures of the films	301:328	Microstructures of the films were performed on atomic force microscope, Fourier transform infrared spectrometer, and X-ray diffraction (XRD).
32758600	4	34	theme	notable	674:680	arg1	change					682:687	not induced notable change	662:687	not induced notable change	662:687	The addition of AOB had not induced notable change on the surface structure features.
32758600	8	35	theme	radicals	1298:1305	arg1	properties					1259:1268	The light barrier properties	1241:1268	The light barrier properties	1241:1268	The light barrier properties and scavenging rate of DPPH radicals were enhanced with the increasing of AOB content.
32758600	8	35	theme	radicals	1298:1305	arg1	rate					1285:1288	scavenging rate	1274:1288	scavenging rate of DPPH radicals	1274:1305	The light barrier properties and scavenging rate of DPPH radicals were enhanced with the increasing of AOB content.
32758600	8	36	theme	DPPH	1293:1296	arg1	radicals					1298:1305	DPPH radicals	1293:1305	DPPH radicals	1293:1305	The light barrier properties and scavenging rate of DPPH radicals were enhanced with the increasing of AOB content.
32758600	9	37	theme	food	1435:1438	arg1	quality					1440:1446	food quality	1435:1446	food quality	1435:1446	Consequently, the antioxidant films utilized for food packaging could protect food quality from the destruction of heat, mechanical force, light, and oxidative free radicals.
32758600	0	38	theme	physical	15:22	arg1	properties					24:33	Structural and physical properties	0:33	Structural and physical properties of carboxymethyl cellulose/gelatin films	0:74	Structural and physical properties of carboxymethyl cellulose/gelatin films functionalized with antioxidant of bamboo leaves.
32758600	6	39	theme	film	975:978	arg1	temperature					956:966	glass transition temperature	939:966	glass transition temperature	939:966	Viscosity and glass transition temperature of the film with 0.2% AOB were 779.2 ± 26.2 mPa·s and 83.24 °C, respectively, which were higher than those of all other samples.
32758600	6	39	theme	film	975:978	arg1	Viscosity					925:933	Viscosity	925:933	Viscosity	925:933	Viscosity and glass transition temperature of the film with 0.2% AOB were 779.2 ± 26.2 mPa·s and 83.24 °C, respectively, which were higher than those of all other samples.
32758600	3	40	theme	vapor	529:533	arg1	viscosity					482:490	the viscosity	478:490	the viscosity of film forming solutions	478:516	The physical properties, including the viscosity of film forming solutions, the water vapor permeability, mechanical properties, thermodynamics and transparence of CMC-Gel films were determined.
32758600	3	40	theme	vapor	529:533	arg1	permeability					535:546	the water vapor permeability	519:546	the water vapor permeability	519:546	The physical properties, including the viscosity of film forming solutions, the water vapor permeability, mechanical properties, thermodynamics and transparence of CMC-Gel films were determined.
32758600	3	40	theme	vapor	529:533	arg1	properties					456:465	The physical properties	443:465	The physical properties	443:465	The physical properties, including the viscosity of film forming solutions, the water vapor permeability, mechanical properties, thermodynamics and transparence of CMC-Gel films were determined.
32758600	5	41	theme	functionalized	903:916	arg1	films					918:922	functionalized films	903:922	functionalized films	903:922	The good compatibility among AOB, CMC and gelatin was illustrated by XRD for that the crystallization disappeared of gelatin in CMC-Gel composite film and no diffraction peaks in functionalized films.
32758600	9	42	theme	free	1517:1520	arg1	radicals					1522:1529	oxidative free radicals	1507:1529	oxidative free radicals	1507:1529	Consequently, the antioxidant films utilized for food packaging could protect food quality from the destruction of heat, mechanical force, light, and oxidative free radicals.
32758600	8	43	theme	barrier	1251:1257	arg1	properties					1259:1268	The light barrier properties	1241:1268	The light barrier properties	1241:1268	The light barrier properties and scavenging rate of DPPH radicals were enhanced with the increasing of AOB content.
32758600	0	44	theme	carboxymethyl	38:50	arg1	films					70:74	carboxymethyl cellulose/gelatin films	38:74	carboxymethyl cellulose/gelatin films	38:74	Structural and physical properties of carboxymethyl cellulose/gelatin films functionalized with antioxidant of bamboo leaves.
32758600	3	45	theme	forming	500:506	arg1	solutions					508:516	film forming solutions	495:516	film forming solutions	495:516	The physical properties, including the viscosity of film forming solutions, the water vapor permeability, mechanical properties, thermodynamics and transparence of CMC-Gel films were determined.
32758600	9	46	theme	force	1489:1493	arg1	destruction					1457:1467	the destruction	1453:1467	the destruction of heat, mechanical force, light, and oxidative free radicals	1453:1529	Consequently, the antioxidant films utilized for food packaging could protect food quality from the destruction of heat, mechanical force, light, and oxidative free radicals.
32758600	4	47	contain	had	658:660	arg2	change					682:687	not induced notable change	662:687	not induced notable change	662:687	The addition of AOB had not induced notable change on the surface structure features.
32758600	4	47	contain	had	658:660	arg1	addition					642:649	The addition	638:649	The addition of AOB	638:656	The addition of AOB had not induced notable change on the surface structure features.
32758600	3	48	theme	CMC-Gel	607:613	arg1	films					615:619	CMC-Gel films	607:619	CMC-Gel films	607:619	The physical properties, including the viscosity of film forming solutions, the water vapor permeability, mechanical properties, thermodynamics and transparence of CMC-Gel films were determined.
32758600	5	49	theme	no	879:880	arg1	diffraction					882:892	no diffraction	879:892	no diffraction	879:892	The good compatibility among AOB, CMC and gelatin was illustrated by XRD for that the crystallization disappeared of gelatin in CMC-Gel composite film and no diffraction peaks in functionalized films.
32758600	8	50	theme	scavenging	1274:1283	arg1	rate					1285:1288	scavenging rate	1274:1288	scavenging rate of DPPH radicals	1274:1305	The light barrier properties and scavenging rate of DPPH radicals were enhanced with the increasing of AOB content.
32758600	4	51	theme	induced	666:672	arg1	change					682:687	not induced notable change	662:687	not induced notable change	662:687	The addition of AOB had not induced notable change on the surface structure features.
32758600	5	52	theme	good	728:731	arg1	compatibility					733:745	The good compatibility	724:745	The good compatibility among AOB, CMC and gelatin	724:772	The good compatibility among AOB, CMC and gelatin was illustrated by XRD for that the crystallization disappeared of gelatin in CMC-Gel composite film and no diffraction peaks in functionalized films.
32758600	3	53	theme	films	615:619	arg1	properties					560:569	mechanical properties	549:569	mechanical properties	549:569	The physical properties, including the viscosity of film forming solutions, the water vapor permeability, mechanical properties, thermodynamics and transparence of CMC-Gel films were determined.
32758600	3	53	theme	films	615:619	arg1	viscosity					482:490	the viscosity	478:490	the viscosity of film forming solutions	478:516	The physical properties, including the viscosity of film forming solutions, the water vapor permeability, mechanical properties, thermodynamics and transparence of CMC-Gel films were determined.
32758600	3	53	theme	films	615:619	arg1	thermodynamics					572:585	thermodynamics	572:585	thermodynamics	572:585	The physical properties, including the viscosity of film forming solutions, the water vapor permeability, mechanical properties, thermodynamics and transparence of CMC-Gel films were determined.
32758600	3	53	theme	films	615:619	arg1	permeability					535:546	the water vapor permeability	519:546	the water vapor permeability	519:546	The physical properties, including the viscosity of film forming solutions, the water vapor permeability, mechanical properties, thermodynamics and transparence of CMC-Gel films were determined.
32758600	3	53	theme	films	615:619	arg1	properties					456:465	The physical properties	443:465	The physical properties	443:465	The physical properties, including the viscosity of film forming solutions, the water vapor permeability, mechanical properties, thermodynamics and transparence of CMC-Gel films were determined.
32758600	3	53	theme	films	615:619	arg1	transparence					591:602	transparence	591:602	transparence	591:602	The physical properties, including the viscosity of film forming solutions, the water vapor permeability, mechanical properties, thermodynamics and transparence of CMC-Gel films were determined.
32758600	5	54	theme	CMC-Gel	852:858	arg1	film					870:873	CMC-Gel composite film	852:873	CMC-Gel composite film	852:873	The good compatibility among AOB, CMC and gelatin was illustrated by XRD for that the crystallization disappeared of gelatin in CMC-Gel composite film and no diffraction peaks in functionalized films.
32758600	2	55	theme	X-ray	418:422	arg1	diffraction					424:434	X-ray diffraction	418:434	X-ray diffraction (XRD)	418:440	Microstructures of the films were performed on atomic force microscope, Fourier transform infrared spectrometer, and X-ray diffraction (XRD).
32758600	2	55	theme	X-ray	418:422	arg1	XRD					437:439	XRD	437:439	XRD	437:439	Microstructures of the films were performed on atomic force microscope, Fourier transform infrared spectrometer, and X-ray diffraction (XRD).
32758600	0	56	theme	films	70:74	arg1	properties					24:33	Structural and physical properties	0:33	Structural and physical properties of carboxymethyl cellulose/gelatin films	0:74	Structural and physical properties of carboxymethyl cellulose/gelatin films functionalized with antioxidant of bamboo leaves.
32758600	1	57	dep	bamboo	187:192	arg1	leaves					194:199	leaves	194:199	leaves	194:199	In this study functional film enrichment with antioxidant of bamboo leaves (AOB) was prepared based on carboxymethyl cellulose-gelatin (CMC-Gel) mixture using casting method.
32758600	2	58	theme	force	355:359	arg1	microscope					361:370	atomic force microscope	348:370	atomic force microscope	348:370	Microstructures of the films were performed on atomic force microscope, Fourier transform infrared spectrometer, and X-ray diffraction (XRD).
32758600	0	59	theme	cellulose/gelatin	52:68	arg1	films					70:74	carboxymethyl cellulose/gelatin films	38:74	carboxymethyl cellulose/gelatin films	38:74	Structural and physical properties of carboxymethyl cellulose/gelatin films functionalized with antioxidant of bamboo leaves.
32758600	6	60	theme	transition	945:954	arg1	temperature					956:966	glass transition temperature	939:966	glass transition temperature	939:966	Viscosity and glass transition temperature of the film with 0.2% AOB were 779.2 ± 26.2 mPa·s and 83.24 °C, respectively, which were higher than those of all other samples.
32758600	2	61	theme	atomic	348:353	arg1	microscope					361:370	atomic force microscope	348:370	atomic force microscope	348:370	Microstructures of the films were performed on atomic force microscope, Fourier transform infrared spectrometer, and X-ray diffraction (XRD).
32758600	6	62	theme	glass	939:943	arg1	temperature					956:966	glass transition temperature	939:966	glass transition temperature	939:966	Viscosity and glass transition temperature of the film with 0.2% AOB were 779.2 ± 26.2 mPa·s and 83.24 °C, respectively, which were higher than those of all other samples.
32758600	5	63	from	gelatin	841:847	arg1	peaks					894:898	CMC-Gel composite film and no diffraction peaks	852:898	CMC-Gel composite film and no diffraction peaks in functionalized films	852:922	The good compatibility among AOB, CMC and gelatin was illustrated by XRD for that the crystallization disappeared of gelatin in CMC-Gel composite film and no diffraction peaks in functionalized films.
32758600	1	64	theme	functional	140:149	arg1	enrichment					156:165	functional film enrichment	140:165	functional film enrichment with antioxidant of bamboo leaves (AOB)	140:205	In this study functional film enrichment with antioxidant of bamboo leaves (AOB) was prepared based on carboxymethyl cellulose-gelatin (CMC-Gel) mixture using casting method.
32758600	9	65	theme	antioxidant	1375:1385	arg1	films					1387:1391	the antioxidant films	1371:1391	the antioxidant films utilized for food packaging	1371:1419	Consequently, the antioxidant films utilized for food packaging could protect food quality from the destruction of heat, mechanical force, light, and oxidative free radicals.
32758600	2	66	dep	Fourier	373:379	arg1	transform					381:389	transform	381:389	transform infrared spectrometer	381:411	Microstructures of the films were performed on atomic force microscope, Fourier transform infrared spectrometer, and X-ray diffraction (XRD).
32758600	9	67	theme	radicals	1522:1529	arg1	destruction					1457:1467	the destruction	1453:1467	the destruction of heat, mechanical force, light, and oxidative free radicals	1453:1529	Consequently, the antioxidant films utilized for food packaging could protect food quality from the destruction of heat, mechanical force, light, and oxidative free radicals.
32758600	7	68	theme	water	1101:1105	arg1	vapor					1107:1111	The water vapor permeation resistance and elongation	1097:1148	vapor	1107:1111	The water vapor permeation resistance and elongation at break of the films significantly decreased when the concentration of AOB exceeded 0.2%.
32758600	1	69	theme	film	151:154	arg1	enrichment					156:165	functional film enrichment	140:165	functional film enrichment with antioxidant of bamboo leaves (AOB)	140:205	In this study functional film enrichment with antioxidant of bamboo leaves (AOB) was prepared based on carboxymethyl cellulose-gelatin (CMC-Gel) mixture using casting method.
32758600	1	70	theme	carboxymethyl	229:241	arg1	mixture					271:277	carboxymethyl cellulose-gelatin (CMC-Gel) mixture	229:277	carboxymethyl cellulose-gelatin (CMC-Gel) mixture using casting method	229:298	In this study functional film enrichment with antioxidant of bamboo leaves (AOB) was prepared based on carboxymethyl cellulose-gelatin (CMC-Gel) mixture using casting method.
32758600	4	71	theme	AOB	654:656	arg1	addition					642:649	The addition	638:649	The addition of AOB	638:656	The addition of AOB had not induced notable change on the surface structure features.
32758600	5	72	theme	diffraction	882:892	arg1	peaks					894:898	CMC-Gel composite film and no diffraction peaks	852:898	CMC-Gel composite film and no diffraction peaks in functionalized films	852:922	The good compatibility among AOB, CMC and gelatin was illustrated by XRD for that the crystallization disappeared of gelatin in CMC-Gel composite film and no diffraction peaks in functionalized films.
32758600	5	73	theme	film	870:873	arg1	peaks					894:898	CMC-Gel composite film and no diffraction peaks	852:898	CMC-Gel composite film and no diffraction peaks in functionalized films	852:922	The good compatibility among AOB, CMC and gelatin was illustrated by XRD for that the crystallization disappeared of gelatin in CMC-Gel composite film and no diffraction peaks in functionalized films.
32758600	1	74	theme	cellulose-gelatin	243:259	arg1	mixture					271:277	carboxymethyl cellulose-gelatin (CMC-Gel) mixture	229:277	carboxymethyl cellulose-gelatin (CMC-Gel) mixture using casting method	229:298	In this study functional film enrichment with antioxidant of bamboo leaves (AOB) was prepared based on carboxymethyl cellulose-gelatin (CMC-Gel) mixture using casting method.
32758600	3	75	theme	water	523:527	arg1	viscosity					482:490	the viscosity	478:490	the viscosity of film forming solutions	478:516	The physical properties, including the viscosity of film forming solutions, the water vapor permeability, mechanical properties, thermodynamics and transparence of CMC-Gel films were determined.
32758600	3	75	theme	water	523:527	arg1	permeability					535:546	the water vapor permeability	519:546	the water vapor permeability	519:546	The physical properties, including the viscosity of film forming solutions, the water vapor permeability, mechanical properties, thermodynamics and transparence of CMC-Gel films were determined.
32758600	3	75	theme	water	523:527	arg1	properties					456:465	The physical properties	443:465	The physical properties	443:465	The physical properties, including the viscosity of film forming solutions, the water vapor permeability, mechanical properties, thermodynamics and transparence of CMC-Gel films were determined.
33231591	2	0	theme	low	573:575	arg1	DF					585:586	low or high DF	573:586	low or high DF	573:586	After 20-weeks of high-fat fructose-rich diet feeding for the development of obesity, forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight) were allocated to one of the four diets with low or high DF and protein contents in a two by two factorial design and digesta were collected from the intestinal segments of minipigs after 8 weeks at libitum feeding.
33231591	0	1	theme	obese	169:173	arg1	model					193:197	obese Göttingen Minipig model	169:197	obese Göttingen Minipig model	169:197	Effects of dietary fibre and protein content on intestinal fibre degradation, short-chain fatty acid and microbiota composition in a high-fat fructose-rich diet induced obese Göttingen Minipig model.
33231591	8	2	from	vein	1652:1655	arg1	concentrations					1610:1623	The butyrate concentrations	1597:1623	The butyrate concentrations in plasma from the jugular vein	1597:1655	The butyrate concentrations in plasma from the jugular vein were increased with high DF diets (P = 0.031), whereas the propionate concentrations were increased (P < 0.001) and succinate were decreased (P = 0.001) with high protein diets compared with low protein diets.
33231591	8	2	from	vein	1652:1655	arg1	plasma					1628:1633	plasma	1628:1633	plasma from the jugular vein	1628:1655	The butyrate concentrations in plasma from the jugular vein were increased with high DF diets (P = 0.031), whereas the propionate concentrations were increased (P < 0.001) and succinate were decreased (P = 0.001) with high protein diets compared with low protein diets.
33231591	1	3	theme	microbiome	260:269	arg1	dysbiosis					271:279	gut microbiome dysbiosis	256:279	gut microbiome dysbiosis	256:279	Obesity-related metabolic syndrome has been linked with gut microbiome dysbiosis while dietary fibre (DF) and protein can modify the gut microbial ecosystem and metabolism.
33231591	2	4	theme	diets	562:566	arg1	one					546:548	one	546:548	one	546:548	After 20-weeks of high-fat fructose-rich diet feeding for the development of obesity, forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight) were allocated to one of the four diets with low or high DF and protein contents in a two by two factorial design and digesta were collected from the intestinal segments of minipigs after 8 weeks at libitum feeding.
33231591	2	4	theme	diets	562:566	arg1	diets					562:566	the four diets	553:566	the four diets with low or high DF and protein contents in a two	553:616	After 20-weeks of high-fat fructose-rich diet feeding for the development of obesity, forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight) were allocated to one of the four diets with low or high DF and protein contents in a two by two factorial design and digesta were collected from the intestinal segments of minipigs after 8 weeks at libitum feeding.
33231591	5	5	from	Peptococcus	1132:1142	arg1	caecum					1151:1156	the caecum	1147:1156	the caecum	1147:1156	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	5	5	from	Peptococcus	1132:1142	arg1	faeces					1177:1182	faeces	1177:1182	faeces	1177:1182	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	5	5	from	Peptococcus	1132:1142	arg1	colon					1167:1171	the mid colon	1159:1171	the mid colon	1159:1171	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	5	6	from	abundance	1089:1097	arg1	caecum					1151:1156	the caecum	1147:1156	the caecum	1147:1156	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	5	6	from	abundance	1089:1097	arg1	faeces					1177:1182	faeces	1177:1182	faeces	1177:1182	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	5	6	from	abundance	1089:1097	arg1	colon					1167:1171	the mid colon	1159:1171	the mid colon	1159:1171	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	0	7	theme	Minipig	185:191	arg1	model					193:197	obese Göttingen Minipig model	169:197	obese Göttingen Minipig model	169:197	Effects of dietary fibre and protein content on intestinal fibre degradation, short-chain fatty acid and microbiota composition in a high-fat fructose-rich diet induced obese Göttingen Minipig model.
33231591	2	8	with	diets	562:566	arg1	DF					585:586	low or high DF	573:586	low or high DF	573:586	After 20-weeks of high-fat fructose-rich diet feeding for the development of obesity, forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight) were allocated to one of the four diets with low or high DF and protein contents in a two by two factorial design and digesta were collected from the intestinal segments of minipigs after 8 weeks at libitum feeding.
33231591	2	8	with	diets	562:566	arg1	contents					600:607	protein contents	592:607	protein contents in a two	592:616	After 20-weeks of high-fat fructose-rich diet feeding for the development of obesity, forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight) were allocated to one of the four diets with low or high DF and protein contents in a two by two factorial design and digesta were collected from the intestinal segments of minipigs after 8 weeks at libitum feeding.
33231591	8	9	theme	protein	1820:1826	arg1	diets					1828:1832	high protein diets	1815:1832	high protein diets compared with low protein diets	1815:1864	The butyrate concentrations in plasma from the jugular vein were increased with high DF diets (P = 0.031), whereas the propionate concentrations were increased (P < 0.001) and succinate were decreased (P = 0.001) with high protein diets compared with low protein diets.
33231591	2	10	theme	minipigs	701:708	arg1	segments					689:696	the intestinal segments	674:696	the intestinal segments of minipigs	674:708	After 20-weeks of high-fat fructose-rich diet feeding for the development of obesity, forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight) were allocated to one of the four diets with low or high DF and protein contents in a two by two factorial design and digesta were collected from the intestinal segments of minipigs after 8 weeks at libitum feeding.
33231591	5	11	theme	total	1226:1230	arg1	0.020					1268:1272	P = 0.020	1264:1272	P = 0.020	1264:1272	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	5	11	theme	total	1226:1230	arg1	SCFA					1257:1260	SCFA	1257:1260	SCFA	1257:1260	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	5	11	theme	total	1226:1230	arg1	acids					1250:1254	total short-chain fatty acids	1226:1254	total short-chain fatty acids (SCFA) (P = 0.020)	1226:1273	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	9	12	theme	improved	2019:2026	arg1	production					2037:2046	slightly improved butyrate production	2010:2046	slightly improved butyrate production	2010:2046	In conclusion, AX in the high DF diets was continuously degraded up to the mid-colon, associated with enriched butyrate-producing bacteria and slightly improved butyrate production, while protein fermentation was attenuated by high DF and high protein did not show prebiotic effects in this obese minipig model.
33231591	2	13	theme	high	580:583	arg1	DF					585:586	low or high DF	573:586	low or high DF	573:586	After 20-weeks of high-fat fructose-rich diet feeding for the development of obesity, forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight) were allocated to one of the four diets with low or high DF and protein contents in a two by two factorial design and digesta were collected from the intestinal segments of minipigs after 8 weeks at libitum feeding.
33231591	7	14	theme	High	1489:1492	arg1	DF					1494:1495	High DF	1489:1495	High DF	1489:1495	High DF induced a lower increase in the BCFA concentration and proportion throughout the colon (P < 0.001).
33231591	5	15	theme	fatty	1244:1248	arg1	0.020					1268:1272	P = 0.020	1264:1272	P = 0.020	1264:1272	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	5	15	theme	fatty	1244:1248	arg1	SCFA					1257:1260	SCFA	1257:1260	SCFA	1257:1260	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	5	15	theme	fatty	1244:1248	arg1	acids					1250:1254	total short-chain fatty acids	1226:1254	total short-chain fatty acids (SCFA) (P = 0.020)	1226:1273	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	8	16	dep	increased	1747:1755	arg1	<					1760:1760	P < 0.001	1758:1766	P < 0.001	1758:1766	The butyrate concentrations in plasma from the jugular vein were increased with high DF diets (P = 0.031), whereas the propionate concentrations were increased (P < 0.001) and succinate were decreased (P = 0.001) with high protein diets compared with low protein diets.
33231591	8	17	theme	low	1848:1850	arg1	diets					1860:1864	low protein diets	1848:1864	low protein diets	1848:1864	The butyrate concentrations in plasma from the jugular vein were increased with high DF diets (P = 0.031), whereas the propionate concentrations were increased (P < 0.001) and succinate were decreased (P = 0.001) with high protein diets compared with low protein diets.
33231591	0	18	theme	microbiota	105:114	arg1	composition					116:126	microbiota composition	105:126	microbiota composition	105:126	Effects of dietary fibre and protein content on intestinal fibre degradation, short-chain fatty acid and microbiota composition in a high-fat fructose-rich diet induced obese Göttingen Minipig model.
33231591	9	19	theme	butyrate-producing	1978:1995	arg1	bacteria					1997:2004	enriched butyrate-producing bacteria	1969:2004	enriched butyrate-producing bacteria	1969:2004	In conclusion, AX in the high DF diets was continuously degraded up to the mid-colon, associated with enriched butyrate-producing bacteria and slightly improved butyrate production, while protein fermentation was attenuated by high DF and high protein did not show prebiotic effects in this obese minipig model.
33231591	1	20	theme	Obesity-related	200:214	arg1	syndrome					226:233	Obesity-related metabolic syndrome	200:233	Obesity-related metabolic syndrome	200:233	Obesity-related metabolic syndrome has been linked with gut microbiome dysbiosis while dietary fibre (DF) and protein can modify the gut microbial ecosystem and metabolism.
33231591	0	21	from	degradation	65:75	arg1	diet					156:159	a high-fat fructose-rich diet	131:159	a high-fat fructose-rich diet	131:159	Effects of dietary fibre and protein content on intestinal fibre degradation, short-chain fatty acid and microbiota composition in a high-fat fructose-rich diet induced obese Göttingen Minipig model.
33231591	6	22	dep	=	1426:1426	arg1	0.026					1428:1432	0.026	1428:1432	0.026	1428:1432	High protein increased the SCFA (P = 0.026) and propionate (P = 0.044) concentrations in the gut.
33231591	6	23	from	gut	1484:1486	arg1	SCFA					1418:1421	SCFA	1418:1421	SCFA	1418:1421	High protein increased the SCFA (P = 0.026) and propionate (P = 0.044) concentrations in the gut.
33231591	6	24	theme	=	1426:1426	arg1	P					1424:1424	P	1424:1424	P = 0.026	1424:1432	High protein increased the SCFA (P = 0.026) and propionate (P = 0.044) concentrations in the gut.
33231591	3	25	from	content	835:841	arg1	segments					897:904	all intestinal segments	882:904	all intestinal segments	882:904	High DF content increased (P < 0.001) while high protein content decreased (P = 0.004) the content of non-starch polysaccharides (NSP) in all intestinal segments.
33231591	2	26	from	contents	600:607	arg1	two					614:616	two	614:616	two	614:616	After 20-weeks of high-fat fructose-rich diet feeding for the development of obesity, forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight) were allocated to one of the four diets with low or high DF and protein contents in a two by two factorial design and digesta were collected from the intestinal segments of minipigs after 8 weeks at libitum feeding.
33231591	0	27	theme	high-fat	133:140	arg1	diet					156:159	a high-fat fructose-rich diet	131:159	a high-fat fructose-rich diet	131:159	Effects of dietary fibre and protein content on intestinal fibre degradation, short-chain fatty acid and microbiota composition in a high-fat fructose-rich diet induced obese Göttingen Minipig model.
33231591	0	28	from	acid	96:99	arg1	diet					156:159	a high-fat fructose-rich diet	131:159	a high-fat fructose-rich diet	131:159	Effects of dietary fibre and protein content on intestinal fibre degradation, short-chain fatty acid and microbiota composition in a high-fat fructose-rich diet induced obese Göttingen Minipig model.
33231591	7	29	theme	lower	1507:1511	arg1	increase					1513:1520	a lower increase	1505:1520	a lower increase in the BCFA concentration	1505:1546	High DF induced a lower increase in the BCFA concentration and proportion throughout the colon (P < 0.001).
33231591	3	30	theme	High	744:747	arg1	content					752:758	High DF content	744:758	High DF content	744:758	High DF content increased (P < 0.001) while high protein content decreased (P = 0.004) the content of non-starch polysaccharides (NSP) in all intestinal segments.
33231591	9	31	theme	high	2094:2097	arg1	DF					2099:2100	high DF	2094:2100	high DF	2094:2100	In conclusion, AX in the high DF diets was continuously degraded up to the mid-colon, associated with enriched butyrate-producing bacteria and slightly improved butyrate production, while protein fermentation was attenuated by high DF and high protein did not show prebiotic effects in this obese minipig model.
33231591	9	32	from	DF	1897:1898	arg1	AX					1882:1883	AX	1882:1883	AX	1882:1883	In conclusion, AX in the high DF diets was continuously degraded up to the mid-colon, associated with enriched butyrate-producing bacteria and slightly improved butyrate production, while protein fermentation was attenuated by high DF and high protein did not show prebiotic effects in this obese minipig model.
33231591	4	33	theme	distal	1005:1010	arg1	intestine					1018:1026	the distal small intestine	1001:1026	the distal small intestine	1001:1026	Arabinoxylan (AX) as proportion of NSP was higher with high DF (P < 0.001) but decreased from the distal small intestine to the mid colon (P < 0.001).
33231591	0	34	theme	intestinal	48:57	arg1	degradation					65:75	intestinal fibre degradation	48:75	intestinal fibre degradation	48:75	Effects of dietary fibre and protein content on intestinal fibre degradation, short-chain fatty acid and microbiota composition in a high-fat fructose-rich diet induced obese Göttingen Minipig model.
33231591	5	35	theme	relative	1080:1087	arg1	abundance					1089:1097	the relative abundance	1076:1097	the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces	1076:1182	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	1	36	theme	gut	333:335	arg1	ecosystem					347:355	the gut microbial ecosystem	329:355	the gut microbial ecosystem	329:355	Obesity-related metabolic syndrome has been linked with gut microbiome dysbiosis while dietary fibre (DF) and protein can modify the gut microbial ecosystem and metabolism.
33231591	7	37	theme	BCFA	1529:1532	arg1	concentration					1534:1546	the BCFA concentration	1525:1546	the BCFA concentration	1525:1546	High DF induced a lower increase in the BCFA concentration and proportion throughout the colon (P < 0.001).
33231591	3	38	theme	high	788:791	arg1	content					801:807	high protein content	788:807	high protein content	788:807	High DF content increased (P < 0.001) while high protein content decreased (P = 0.004) the content of non-starch polysaccharides (NSP) in all intestinal segments.
33231591	2	39	theme	body	515:518	arg1	weight					520:525	31 ± 4.0 kg body weight	503:525	31 ± 4.0 kg body weight	503:525	After 20-weeks of high-fat fructose-rich diet feeding for the development of obesity, forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight) were allocated to one of the four diets with low or high DF and protein contents in a two by two factorial design and digesta were collected from the intestinal segments of minipigs after 8 weeks at libitum feeding.
33231591	2	39	theme	body	515:518	arg1	Minipigs					493:500	forty-three 30-week old Göttingen Minipigs	459:500	forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight)	459:526	After 20-weeks of high-fat fructose-rich diet feeding for the development of obesity, forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight) were allocated to one of the four diets with low or high DF and protein contents in a two by two factorial design and digesta were collected from the intestinal segments of minipigs after 8 weeks at libitum feeding.
33231591	0	40	from	Effects	0:6	arg1	degradation					65:75	intestinal fibre degradation	48:75	intestinal fibre degradation	48:75	Effects of dietary fibre and protein content on intestinal fibre degradation, short-chain fatty acid and microbiota composition in a high-fat fructose-rich diet induced obese Göttingen Minipig model.
33231591	0	40	from	Effects	0:6	arg1	acid					96:99	short-chain fatty acid	78:99	short-chain fatty acid	78:99	Effects of dietary fibre and protein content on intestinal fibre degradation, short-chain fatty acid and microbiota composition in a high-fat fructose-rich diet induced obese Göttingen Minipig model.
33231591	0	40	from	Effects	0:6	arg1	composition					116:126	microbiota composition	105:126	microbiota composition	105:126	Effects of dietary fibre and protein content on intestinal fibre degradation, short-chain fatty acid and microbiota composition in a high-fat fructose-rich diet induced obese Göttingen Minipig model.
33231591	9	41	theme	AX	1882:1883	arg1	diets					1900:1904	AX in the high DF diets	1882:1904	AX in the high DF diets	1882:1904	In conclusion, AX in the high DF diets was continuously degraded up to the mid-colon, associated with enriched butyrate-producing bacteria and slightly improved butyrate production, while protein fermentation was attenuated by high DF and high protein did not show prebiotic effects in this obese minipig model.
33231591	0	42	theme	short-chain	78:88	arg1	acid					96:99	short-chain fatty acid	78:99	short-chain fatty acid	78:99	Effects of dietary fibre and protein content on intestinal fibre degradation, short-chain fatty acid and microbiota composition in a high-fat fructose-rich diet induced obese Göttingen Minipig model.
33231591	6	43	theme	High	1391:1394	arg1	protein					1396:1402	High protein	1391:1402	High protein	1391:1402	High protein increased the SCFA (P = 0.026) and propionate (P = 0.044) concentrations in the gut.
33231591	3	44	theme	=	822:822	arg1	0.004					824:828	P = 0.004	820:828	P = 0.004	820:828	High DF content increased (P < 0.001) while high protein content decreased (P = 0.004) the content of non-starch polysaccharides (NSP) in all intestinal segments.
33231591	6	45	dep	concentrations	1462:1475	arg1	P					1451:1451	P	1451:1451	P = 0.044	1451:1459	High protein increased the SCFA (P = 0.026) and propionate (P = 0.044) concentrations in the gut.
33231591	5	46	theme	P	1264:1264	arg1	0.020					1268:1272	P = 0.020	1264:1272	P = 0.020	1264:1272	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	5	46	theme	P	1264:1264	arg1	acids					1250:1254	total short-chain fatty acids	1226:1254	total short-chain fatty acids (SCFA) (P = 0.020)	1226:1273	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	2	47	theme	protein	592:598	arg1	contents					600:607	protein contents	592:607	protein contents in a two	592:616	After 20-weeks of high-fat fructose-rich diet feeding for the development of obesity, forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight) were allocated to one of the four diets with low or high DF and protein contents in a two by two factorial design and digesta were collected from the intestinal segments of minipigs after 8 weeks at libitum feeding.
33231591	2	48	theme	high-fat	391:398	arg1	feeding					419:425	high-fat fructose-rich diet feeding	391:425	high-fat fructose-rich diet feeding	391:425	After 20-weeks of high-fat fructose-rich diet feeding for the development of obesity, forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight) were allocated to one of the four diets with low or high DF and protein contents in a two by two factorial design and digesta were collected from the intestinal segments of minipigs after 8 weeks at libitum feeding.
33231591	6	49	from	SCFA	1418:1421	arg1	gut					1484:1486	the gut	1480:1486	the gut	1480:1486	High protein increased the SCFA (P = 0.026) and propionate (P = 0.044) concentrations in the gut.
33231591	2	50	theme	obesity	450:456	arg1	development					435:445	the development	431:445	the development of obesity	431:456	After 20-weeks of high-fat fructose-rich diet feeding for the development of obesity, forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight) were allocated to one of the four diets with low or high DF and protein contents in a two by two factorial design and digesta were collected from the intestinal segments of minipigs after 8 weeks at libitum feeding.
33231591	6	51	theme	propionate	1439:1448	arg1	concentrations					1462:1475	the SCFA (P = 0.026) and propionate (P = 0.044) concentrations	1414:1475	concentrations	1462:1475	High protein increased the SCFA (P = 0.026) and propionate (P = 0.044) concentrations in the gut.
33231591	5	52	from	faeces	1177:1182	arg1	abundance					1089:1097	the relative abundance	1076:1097	the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces	1076:1182	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	5	52	from	faeces	1177:1182	arg1	Peptococcus					1132:1142	Peptococcus	1132:1142	Peptococcus	1132:1142	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	9	53	theme	obese	2158:2162	arg1	model					2172:2176	this obese minipig model	2153:2176	this obese minipig model	2153:2176	In conclusion, AX in the high DF diets was continuously degraded up to the mid-colon, associated with enriched butyrate-producing bacteria and slightly improved butyrate production, while protein fermentation was attenuated by high DF and high protein did not show prebiotic effects in this obese minipig model.
33231591	8	54	theme	P	1758:1758	arg1	<					1760:1760	P < 0.001	1758:1766	P < 0.001	1758:1766	The butyrate concentrations in plasma from the jugular vein were increased with high DF diets (P = 0.031), whereas the propionate concentrations were increased (P < 0.001) and succinate were decreased (P = 0.001) with high protein diets compared with low protein diets.
33231591	2	55	theme	old	479:481	arg1	weight					520:525	31 ± 4.0 kg body weight	503:525	31 ± 4.0 kg body weight	503:525	After 20-weeks of high-fat fructose-rich diet feeding for the development of obesity, forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight) were allocated to one of the four diets with low or high DF and protein contents in a two by two factorial design and digesta were collected from the intestinal segments of minipigs after 8 weeks at libitum feeding.
33231591	2	55	theme	old	479:481	arg1	Minipigs					493:500	forty-three 30-week old Göttingen Minipigs	459:500	forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight)	459:526	After 20-weeks of high-fat fructose-rich diet feeding for the development of obesity, forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight) were allocated to one of the four diets with low or high DF and protein contents in a two by two factorial design and digesta were collected from the intestinal segments of minipigs after 8 weeks at libitum feeding.
33231591	5	56	theme	butyrate	1322:1329	arg1	pools					1331:1335	butyrate pools	1322:1335	butyrate pools	1322:1335	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	2	57	theme	diet	414:417	arg1	feeding					419:425	high-fat fructose-rich diet feeding	391:425	high-fat fructose-rich diet feeding	391:425	After 20-weeks of high-fat fructose-rich diet feeding for the development of obesity, forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight) were allocated to one of the four diets with low or high DF and protein contents in a two by two factorial design and digesta were collected from the intestinal segments of minipigs after 8 weeks at libitum feeding.
33231591	2	58	theme	forty-three	459:469	arg1	weight					520:525	31 ± 4.0 kg body weight	503:525	31 ± 4.0 kg body weight	503:525	After 20-weeks of high-fat fructose-rich diet feeding for the development of obesity, forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight) were allocated to one of the four diets with low or high DF and protein contents in a two by two factorial design and digesta were collected from the intestinal segments of minipigs after 8 weeks at libitum feeding.
33231591	2	58	theme	forty-three	459:469	arg1	Minipigs					493:500	forty-three 30-week old Göttingen Minipigs	459:500	forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight)	459:526	After 20-weeks of high-fat fructose-rich diet feeding for the development of obesity, forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight) were allocated to one of the four diets with low or high DF and protein contents in a two by two factorial design and digesta were collected from the intestinal segments of minipigs after 8 weeks at libitum feeding.
33231591	5	59	theme	=	1290:1290	arg1	acetate					1279:1285	acetate	1279:1285	acetate (P = 0.011)	1279:1297	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	5	59	theme	=	1290:1290	arg1	0.011					1292:1296	P = 0.011	1288:1296	P = 0.011	1288:1296	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	5	60	theme	intestinal	1197:1206	arg1	concentrations					1208:1221	the intestinal concentrations	1193:1221	the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011)	1193:1297	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	0	61	theme	content	37:43	arg1	Effects					0:6	Effects	0:6	Effects of dietary fibre and protein content on intestinal fibre degradation, short-chain fatty acid and microbiota composition in a high-fat fructose-rich diet	0:159	Effects of dietary fibre and protein content on intestinal fibre degradation, short-chain fatty acid and microbiota composition in a high-fat fructose-rich diet induced obese Göttingen Minipig model.
33231591	7	62	theme	P	1585:1585	arg1	colon					1578:1582	the colon	1574:1582	the colon (P < 0.001)	1574:1594	High DF induced a lower increase in the BCFA concentration and proportion throughout the colon (P < 0.001).
33231591	7	62	theme	P	1585:1585	arg1	<					1587:1587	P < 0.001	1585:1593	P < 0.001	1585:1593	High DF induced a lower increase in the BCFA concentration and proportion throughout the colon (P < 0.001).
33231591	3	63	theme	non-starch	846:855	arg1	NSP					874:876	NSP	874:876	NSP	874:876	High DF content increased (P < 0.001) while high protein content decreased (P = 0.004) the content of non-starch polysaccharides (NSP) in all intestinal segments.
33231591	3	63	theme	non-starch	846:855	arg1	polysaccharides					857:871	non-starch polysaccharides	846:871	non-starch polysaccharides (NSP)	846:877	High DF content increased (P < 0.001) while high protein content decreased (P = 0.004) the content of non-starch polysaccharides (NSP) in all intestinal segments.
33231591	4	64	theme	high	962:965	arg1	P					971:971	P < 0.001	971:979	P < 0.001	971:979	Arabinoxylan (AX) as proportion of NSP was higher with high DF (P < 0.001) but decreased from the distal small intestine to the mid colon (P < 0.001).
33231591	4	64	theme	high	962:965	arg1	DF					967:968	high DF	962:968	high DF (P < 0.001)	962:980	Arabinoxylan (AX) as proportion of NSP was higher with high DF (P < 0.001) but decreased from the distal small intestine to the mid colon (P < 0.001).
33231591	2	65	from	DF	585:586	arg1	two					614:616	two	614:616	two	614:616	After 20-weeks of high-fat fructose-rich diet feeding for the development of obesity, forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight) were allocated to one of the four diets with low or high DF and protein contents in a two by two factorial design and digesta were collected from the intestinal segments of minipigs after 8 weeks at libitum feeding.
33231591	8	66	from	concentrations	1610:1623	arg1	vein					1652:1655	the jugular vein	1640:1655	the jugular vein	1640:1655	The butyrate concentrations in plasma from the jugular vein were increased with high DF diets (P = 0.031), whereas the propionate concentrations were increased (P < 0.001) and succinate were decreased (P = 0.001) with high protein diets compared with low protein diets.
33231591	8	66	from	concentrations	1610:1623	arg1	plasma					1628:1633	plasma	1628:1633	plasma from the jugular vein	1628:1655	The butyrate concentrations in plasma from the jugular vein were increased with high DF diets (P = 0.031), whereas the propionate concentrations were increased (P < 0.001) and succinate were decreased (P = 0.001) with high protein diets compared with low protein diets.
33231591	4	67	theme	NSP	942:944	arg1	proportion					928:937	Arabinoxylan (AX) as proportion	907:937	Arabinoxylan (AX) as proportion of NSP	907:944	Arabinoxylan (AX) as proportion of NSP was higher with high DF (P < 0.001) but decreased from the distal small intestine to the mid colon (P < 0.001).
33231591	9	68	theme	enriched	1969:1976	arg1	bacteria					1997:2004	enriched butyrate-producing bacteria	1969:2004	enriched butyrate-producing bacteria	1969:2004	In conclusion, AX in the high DF diets was continuously degraded up to the mid-colon, associated with enriched butyrate-producing bacteria and slightly improved butyrate production, while protein fermentation was attenuated by high DF and high protein did not show prebiotic effects in this obese minipig model.
33231591	1	69	theme	gut	256:258	arg1	dysbiosis					271:279	gut microbiome dysbiosis	256:279	gut microbiome dysbiosis	256:279	Obesity-related metabolic syndrome has been linked with gut microbiome dysbiosis while dietary fibre (DF) and protein can modify the gut microbial ecosystem and metabolism.
33231591	5	70	theme	large	1344:1348	arg1	P≤					1361:1362	P≤	1361:1362	P≤	1361:1362	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	5	70	theme	large	1344:1348	arg1	intestine					1350:1358	the large intestine	1340:1358	the large intestine (P≤ 0.050) compared to low DF	1340:1388	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	4	71	dep	intestine	1018:1026	arg1	P					1046:1046	P < 0.001	1046:1054	P < 0.001	1046:1054	Arabinoxylan (AX) as proportion of NSP was higher with high DF (P < 0.001) but decreased from the distal small intestine to the mid colon (P < 0.001).
33231591	8	72	dep	increased	1662:1670	arg1	whereas					1704:1710	whereas	1704:1710	whereas	1704:1710	The butyrate concentrations in plasma from the jugular vein were increased with high DF diets (P = 0.031), whereas the propionate concentrations were increased (P < 0.001) and succinate were decreased (P = 0.001) with high protein diets compared with low protein diets.
33231591	8	73	theme	high	1815:1818	arg1	diets					1828:1832	high protein diets	1815:1832	high protein diets compared with low protein diets	1815:1864	The butyrate concentrations in plasma from the jugular vein were increased with high DF diets (P = 0.031), whereas the propionate concentrations were increased (P < 0.001) and succinate were decreased (P = 0.001) with high protein diets compared with low protein diets.
33231591	5	74	theme	short-chain	1232:1242	arg1	0.020					1268:1272	P = 0.020	1264:1272	P = 0.020	1264:1272	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	5	74	theme	short-chain	1232:1242	arg1	SCFA					1257:1260	SCFA	1257:1260	SCFA	1257:1260	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	5	74	theme	short-chain	1232:1242	arg1	acids					1250:1254	total short-chain fatty acids	1226:1254	total short-chain fatty acids (SCFA) (P = 0.020)	1226:1273	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	9	75	theme	butyrate	2028:2035	arg1	production					2037:2046	slightly improved butyrate production	2010:2046	slightly improved butyrate production	2010:2046	In conclusion, AX in the high DF diets was continuously degraded up to the mid-colon, associated with enriched butyrate-producing bacteria and slightly improved butyrate production, while protein fermentation was attenuated by high DF and high protein did not show prebiotic effects in this obese minipig model.
33231591	6	76	dep	SCFA	1418:1421	arg1	P					1424:1424	P	1424:1424	P = 0.026	1424:1432	High protein increased the SCFA (P = 0.026) and propionate (P = 0.044) concentrations in the gut.
33231591	2	77	theme	libitum	727:733	arg1	feeding					735:741	libitum feeding	727:741	libitum feeding	727:741	After 20-weeks of high-fat fructose-rich diet feeding for the development of obesity, forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight) were allocated to one of the four diets with low or high DF and protein contents in a two by two factorial design and digesta were collected from the intestinal segments of minipigs after 8 weeks at libitum feeding.
33231591	8	78	theme	butyrate	1601:1608	arg1	concentrations					1610:1623	The butyrate concentrations	1597:1623	The butyrate concentrations in plasma from the jugular vein	1597:1655	The butyrate concentrations in plasma from the jugular vein were increased with high DF diets (P = 0.031), whereas the propionate concentrations were increased (P < 0.001) and succinate were decreased (P = 0.001) with high protein diets compared with low protein diets.
33231591	5	79	theme	acids	1250:1254	arg1	concentrations					1208:1221	the intestinal concentrations	1193:1221	the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011)	1193:1297	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	4	80	theme	<	973:973	arg1	P					971:971	P < 0.001	971:979	P < 0.001	971:979	Arabinoxylan (AX) as proportion of NSP was higher with high DF (P < 0.001) but decreased from the distal small intestine to the mid colon (P < 0.001).
33231591	4	80	theme	<	973:973	arg1	DF					967:968	high DF	962:968	high DF (P < 0.001)	962:980	Arabinoxylan (AX) as proportion of NSP was higher with high DF (P < 0.001) but decreased from the distal small intestine to the mid colon (P < 0.001).
33231591	9	81	theme	protein	2055:2061	arg1	fermentation					2063:2074	protein fermentation	2055:2074	protein fermentation	2055:2074	In conclusion, AX in the high DF diets was continuously degraded up to the mid-colon, associated with enriched butyrate-producing bacteria and slightly improved butyrate production, while protein fermentation was attenuated by high DF and high protein did not show prebiotic effects in this obese minipig model.
33231591	1	82	theme	metabolic	216:224	arg1	syndrome					226:233	Obesity-related metabolic syndrome	200:233	Obesity-related metabolic syndrome	200:233	Obesity-related metabolic syndrome has been linked with gut microbiome dysbiosis while dietary fibre (DF) and protein can modify the gut microbial ecosystem and metabolism.
33231591	5	83	from	caecum	1151:1156	arg1	abundance					1089:1097	the relative abundance	1076:1097	the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces	1076:1182	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	5	83	from	caecum	1151:1156	arg1	Peptococcus					1132:1142	Peptococcus	1132:1142	Peptococcus	1132:1142	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	4	84	theme	mid	1035:1037	arg1	colon					1039:1043	the mid colon	1031:1043	the mid colon	1031:1043	Arabinoxylan (AX) as proportion of NSP was higher with high DF (P < 0.001) but decreased from the distal small intestine to the mid colon (P < 0.001).
33231591	5	85	theme	low	1383:1385	arg1	DF					1387:1388	low DF	1383:1388	low DF	1383:1388	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	8	86	theme	DF	1682:1683	arg1	P					1692:1692	P = 0.031	1692:1700	P = 0.031	1692:1700	The butyrate concentrations in plasma from the jugular vein were increased with high DF diets (P = 0.031), whereas the propionate concentrations were increased (P < 0.001) and succinate were decreased (P = 0.001) with high protein diets compared with low protein diets.
33231591	8	86	theme	DF	1682:1683	arg1	diets					1685:1689	high DF diets	1677:1689	high DF diets (P = 0.031)	1677:1701	The butyrate concentrations in plasma from the jugular vein were increased with high DF diets (P = 0.031), whereas the propionate concentrations were increased (P < 0.001) and succinate were decreased (P = 0.001) with high protein diets compared with low protein diets.
33231591	0	87	theme	fructose-rich	142:154	arg1	diet					156:159	a high-fat fructose-rich diet	131:159	a high-fat fructose-rich diet	131:159	Effects of dietary fibre and protein content on intestinal fibre degradation, short-chain fatty acid and microbiota composition in a high-fat fructose-rich diet induced obese Göttingen Minipig model.
33231591	9	88	from	AX	1882:1883	arg1	DF					1897:1898	the high DF	1888:1898	the high DF	1888:1898	In conclusion, AX in the high DF diets was continuously degraded up to the mid-colon, associated with enriched butyrate-producing bacteria and slightly improved butyrate production, while protein fermentation was attenuated by high DF and high protein did not show prebiotic effects in this obese minipig model.
33231591	8	89	theme	jugular	1644:1650	arg1	vein					1652:1655	the jugular vein	1640:1655	the jugular vein	1640:1655	The butyrate concentrations in plasma from the jugular vein were increased with high DF diets (P = 0.031), whereas the propionate concentrations were increased (P < 0.001) and succinate were decreased (P = 0.001) with high protein diets compared with low protein diets.
33231591	3	90	dep	increased	760:768	arg1	<					773:773	P < 0.001	771:779	P < 0.001	771:779	High DF content increased (P < 0.001) while high protein content decreased (P = 0.004) the content of non-starch polysaccharides (NSP) in all intestinal segments.
33231591	5	91	from	colon	1167:1171	arg1	abundance					1089:1097	the relative abundance	1076:1097	the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces	1076:1182	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	5	91	from	colon	1167:1171	arg1	Peptococcus					1132:1142	Peptococcus	1132:1142	Peptococcus	1132:1142	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	4	92	theme	small	1012:1016	arg1	intestine					1018:1026	the distal small intestine	1001:1026	the distal small intestine	1001:1026	Arabinoxylan (AX) as proportion of NSP was higher with high DF (P < 0.001) but decreased from the distal small intestine to the mid colon (P < 0.001).
33231591	5	93	theme	Faecalibacterium	1111:1126	arg1	abundance					1089:1097	the relative abundance	1076:1097	the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces	1076:1182	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	3	94	theme	DF	749:750	arg1	content					752:758	High DF content	744:758	High DF content	744:758	High DF content increased (P < 0.001) while high protein content decreased (P = 0.004) the content of non-starch polysaccharides (NSP) in all intestinal segments.
33231591	0	95	theme	fibre	59:63	arg1	degradation					65:75	intestinal fibre degradation	48:75	intestinal fibre degradation	48:75	Effects of dietary fibre and protein content on intestinal fibre degradation, short-chain fatty acid and microbiota composition in a high-fat fructose-rich diet induced obese Göttingen Minipig model.
33231591	6	96	dep	=	1453:1453	arg1	0.044					1455:1459	0.044	1455:1459	0.044	1455:1459	High protein increased the SCFA (P = 0.026) and propionate (P = 0.044) concentrations in the gut.
33231591	8	97	theme	high	1677:1680	arg1	P					1692:1692	P = 0.031	1692:1700	P = 0.031	1692:1700	The butyrate concentrations in plasma from the jugular vein were increased with high DF diets (P = 0.031), whereas the propionate concentrations were increased (P < 0.001) and succinate were decreased (P = 0.001) with high protein diets compared with low protein diets.
33231591	8	97	theme	high	1677:1680	arg1	diets					1685:1689	high DF diets	1677:1689	high DF diets (P = 0.031)	1677:1701	The butyrate concentrations in plasma from the jugular vein were increased with high DF diets (P = 0.031), whereas the propionate concentrations were increased (P < 0.001) and succinate were decreased (P = 0.001) with high protein diets compared with low protein diets.
33231591	9	98	theme	high	2106:2109	arg1	protein					2111:2117	high protein	2106:2117	high protein	2106:2117	In conclusion, AX in the high DF diets was continuously degraded up to the mid-colon, associated with enriched butyrate-producing bacteria and slightly improved butyrate production, while protein fermentation was attenuated by high DF and high protein did not show prebiotic effects in this obese minipig model.
33231591	2	99	theme	intestinal	678:687	arg1	segments					689:696	the intestinal segments	674:696	the intestinal segments of minipigs	674:708	After 20-weeks of high-fat fructose-rich diet feeding for the development of obesity, forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight) were allocated to one of the four diets with low or high DF and protein contents in a two by two factorial design and digesta were collected from the intestinal segments of minipigs after 8 weeks at libitum feeding.
33231591	2	100	theme	Göttingen	483:491	arg1	weight					520:525	31 ± 4.0 kg body weight	503:525	31 ± 4.0 kg body weight	503:525	After 20-weeks of high-fat fructose-rich diet feeding for the development of obesity, forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight) were allocated to one of the four diets with low or high DF and protein contents in a two by two factorial design and digesta were collected from the intestinal segments of minipigs after 8 weeks at libitum feeding.
33231591	2	100	theme	Göttingen	483:491	arg1	Minipigs					493:500	forty-three 30-week old Göttingen Minipigs	459:500	forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight)	459:526	After 20-weeks of high-fat fructose-rich diet feeding for the development of obesity, forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight) were allocated to one of the four diets with low or high DF and protein contents in a two by two factorial design and digesta were collected from the intestinal segments of minipigs after 8 weeks at libitum feeding.
33231591	5	101	theme	acetate	1279:1285	arg1	concentrations					1208:1221	the intestinal concentrations	1193:1221	the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011)	1193:1297	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	3	102	theme	P	771:771	arg1	<					773:773	P < 0.001	771:779	P < 0.001	771:779	High DF content increased (P < 0.001) while high protein content decreased (P = 0.004) the content of non-starch polysaccharides (NSP) in all intestinal segments.
33231591	0	103	theme	fatty	90:94	arg1	acid					96:99	short-chain fatty acid	78:99	short-chain fatty acid	78:99	Effects of dietary fibre and protein content on intestinal fibre degradation, short-chain fatty acid and microbiota composition in a high-fat fructose-rich diet induced obese Göttingen Minipig model.
33231591	1	104	theme	microbial	337:345	arg1	ecosystem					347:355	the gut microbial ecosystem	329:355	the gut microbial ecosystem	329:355	Obesity-related metabolic syndrome has been linked with gut microbiome dysbiosis while dietary fibre (DF) and protein can modify the gut microbial ecosystem and metabolism.
33231591	3	105	theme	protein	793:799	arg1	content					801:807	high protein content	788:807	high protein content	788:807	High DF content increased (P < 0.001) while high protein content decreased (P = 0.004) the content of non-starch polysaccharides (NSP) in all intestinal segments.
33231591	6	106	theme	=	1453:1453	arg1	P					1451:1451	P	1451:1451	P = 0.044	1451:1459	High protein increased the SCFA (P = 0.026) and propionate (P = 0.044) concentrations in the gut.
33231591	5	107	theme	P	1288:1288	arg1	acetate					1279:1285	acetate	1279:1285	acetate (P = 0.011)	1279:1297	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	5	107	theme	P	1288:1288	arg1	0.011					1292:1296	P = 0.011	1288:1296	P = 0.011	1288:1296	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	2	108	from	feeding	735:741	arg1	weeks					718:722	8 weeks	716:722	8 weeks at libitum feeding	716:741	After 20-weeks of high-fat fructose-rich diet feeding for the development of obesity, forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight) were allocated to one of the four diets with low or high DF and protein contents in a two by two factorial design and digesta were collected from the intestinal segments of minipigs after 8 weeks at libitum feeding.
33231591	7	109	from	increase	1513:1520	arg1	concentration					1534:1546	the BCFA concentration	1525:1546	the BCFA concentration	1525:1546	High DF induced a lower increase in the BCFA concentration and proportion throughout the colon (P < 0.001).
33231591	5	110	theme	=	1266:1266	arg1	0.020					1268:1272	P = 0.020	1264:1272	P = 0.020	1264:1272	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	5	110	theme	=	1266:1266	arg1	acids					1250:1254	total short-chain fatty acids	1226:1254	total short-chain fatty acids (SCFA) (P = 0.020)	1226:1273	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	2	111	theme	factorial	625:633	arg1	design					635:640	two factorial design	621:640	two factorial design	621:640	After 20-weeks of high-fat fructose-rich diet feeding for the development of obesity, forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight) were allocated to one of the four diets with low or high DF and protein contents in a two by two factorial design and digesta were collected from the intestinal segments of minipigs after 8 weeks at libitum feeding.
33231591	5	112	theme	mid	1163:1165	arg1	colon					1167:1171	the mid colon	1159:1171	the mid colon	1159:1171	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	9	113	theme	high	1892:1895	arg1	DF					1897:1898	the high DF	1888:1898	the high DF	1888:1898	In conclusion, AX in the high DF diets was continuously degraded up to the mid-colon, associated with enriched butyrate-producing bacteria and slightly improved butyrate production, while protein fermentation was attenuated by high DF and high protein did not show prebiotic effects in this obese minipig model.
33231591	8	114	theme	=	1694:1694	arg1	P					1692:1692	P = 0.031	1692:1700	P = 0.031	1692:1700	The butyrate concentrations in plasma from the jugular vein were increased with high DF diets (P = 0.031), whereas the propionate concentrations were increased (P < 0.001) and succinate were decreased (P = 0.001) with high protein diets compared with low protein diets.
33231591	8	114	theme	=	1694:1694	arg1	diets					1685:1689	high DF diets	1677:1689	high DF diets (P = 0.031)	1677:1701	The butyrate concentrations in plasma from the jugular vein were increased with high DF diets (P = 0.031), whereas the propionate concentrations were increased (P < 0.001) and succinate were decreased (P = 0.001) with high protein diets compared with low protein diets.
33231591	3	115	theme	P	820:820	arg1	0.004					824:828	P = 0.004	820:828	P = 0.004	820:828	High DF content increased (P < 0.001) while high protein content decreased (P = 0.004) the content of non-starch polysaccharides (NSP) in all intestinal segments.
33231591	4	116	theme	AX	921:922	arg1	proportion					928:937	Arabinoxylan (AX) as proportion	907:937	Arabinoxylan (AX) as proportion of NSP	907:944	Arabinoxylan (AX) as proportion of NSP was higher with high DF (P < 0.001) but decreased from the distal small intestine to the mid colon (P < 0.001).
33231591	5	117	theme	Peptococcus	1132:1142	arg1	abundance					1089:1097	the relative abundance	1076:1097	the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces	1076:1182	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	0	118	theme	fibre	19:23	arg1	Effects					0:6	Effects	0:6	Effects of dietary fibre and protein content on intestinal fibre degradation, short-chain fatty acid and microbiota composition in a high-fat fructose-rich diet	0:159	Effects of dietary fibre and protein content on intestinal fibre degradation, short-chain fatty acid and microbiota composition in a high-fat fructose-rich diet induced obese Göttingen Minipig model.
33231591	3	119	dep	decreased	809:817	arg1	0.004					824:828	P = 0.004	820:828	P = 0.004	820:828	High DF content increased (P < 0.001) while high protein content decreased (P = 0.004) the content of non-starch polysaccharides (NSP) in all intestinal segments.
33231591	4	120	theme	as	925:926	arg1	proportion					928:937	Arabinoxylan (AX) as proportion	907:937	Arabinoxylan (AX) as proportion of NSP	907:944	Arabinoxylan (AX) as proportion of NSP was higher with high DF (P < 0.001) but decreased from the distal small intestine to the mid colon (P < 0.001).
33231591	2	121	theme	feeding	419:425	arg1	20-weeks					379:386	20-weeks	379:386	20-weeks of high-fat fructose-rich diet feeding for the development of obesity	379:456	After 20-weeks of high-fat fructose-rich diet feeding for the development of obesity, forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight) were allocated to one of the four diets with low or high DF and protein contents in a two by two factorial design and digesta were collected from the intestinal segments of minipigs after 8 weeks at libitum feeding.
33231591	0	122	theme	protein	29:35	arg1	content					37:43	protein content	29:43	protein content	29:43	Effects of dietary fibre and protein content on intestinal fibre degradation, short-chain fatty acid and microbiota composition in a high-fat fructose-rich diet induced obese Göttingen Minipig model.
33231591	9	123	theme	minipig	2164:2170	arg1	model					2172:2176	this obese minipig model	2153:2176	this obese minipig model	2153:2176	In conclusion, AX in the high DF diets was continuously degraded up to the mid-colon, associated with enriched butyrate-producing bacteria and slightly improved butyrate production, while protein fermentation was attenuated by high DF and high protein did not show prebiotic effects in this obese minipig model.
33231591	7	124	from	proportion	1552:1561	arg1	concentration					1534:1546	the BCFA concentration	1525:1546	the BCFA concentration	1525:1546	High DF induced a lower increase in the BCFA concentration and proportion throughout the colon (P < 0.001).
33231591	2	125	theme	30-week	471:477	arg1	weight					520:525	31 ± 4.0 kg body weight	503:525	31 ± 4.0 kg body weight	503:525	After 20-weeks of high-fat fructose-rich diet feeding for the development of obesity, forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight) were allocated to one of the four diets with low or high DF and protein contents in a two by two factorial design and digesta were collected from the intestinal segments of minipigs after 8 weeks at libitum feeding.
33231591	2	125	theme	30-week	471:477	arg1	Minipigs					493:500	forty-three 30-week old Göttingen Minipigs	459:500	forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight)	459:526	After 20-weeks of high-fat fructose-rich diet feeding for the development of obesity, forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight) were allocated to one of the four diets with low or high DF and protein contents in a two by two factorial design and digesta were collected from the intestinal segments of minipigs after 8 weeks at libitum feeding.
33231591	2	126	theme	fructose-rich	400:412	arg1	feeding					419:425	high-fat fructose-rich diet feeding	391:425	high-fat fructose-rich diet feeding	391:425	After 20-weeks of high-fat fructose-rich diet feeding for the development of obesity, forty-three 30-week old Göttingen Minipigs (31 ± 4.0 kg body weight) were allocated to one of the four diets with low or high DF and protein contents in a two by two factorial design and digesta were collected from the intestinal segments of minipigs after 8 weeks at libitum feeding.
33231591	8	127	theme	propionate	1716:1725	arg1	concentrations					1727:1740	the propionate concentrations	1712:1740	the propionate concentrations	1712:1740	The butyrate concentrations in plasma from the jugular vein were increased with high DF diets (P = 0.031), whereas the propionate concentrations were increased (P < 0.001) and succinate were decreased (P = 0.001) with high protein diets compared with low protein diets.
33231591	4	128	theme	Arabinoxylan	907:918	arg1	proportion					928:937	Arabinoxylan (AX) as proportion	907:937	Arabinoxylan (AX) as proportion of NSP	907:944	Arabinoxylan (AX) as proportion of NSP was higher with high DF (P < 0.001) but decreased from the distal small intestine to the mid colon (P < 0.001).
33231591	8	129	theme	protein	1852:1858	arg1	diets					1860:1864	low protein diets	1848:1864	low protein diets	1848:1864	The butyrate concentrations in plasma from the jugular vein were increased with high DF diets (P = 0.031), whereas the propionate concentrations were increased (P < 0.001) and succinate were decreased (P = 0.001) with high protein diets compared with low protein diets.
33231591	9	130	theme	prebiotic	2132:2140	arg1	effects					2142:2148	prebiotic effects	2132:2148	prebiotic effects	2132:2148	In conclusion, AX in the high DF diets was continuously degraded up to the mid-colon, associated with enriched butyrate-producing bacteria and slightly improved butyrate production, while protein fermentation was attenuated by high DF and high protein did not show prebiotic effects in this obese minipig model.
33231591	4	131	theme	<	1048:1048	arg1	P					1046:1046	P < 0.001	1046:1054	P < 0.001	1046:1054	Arabinoxylan (AX) as proportion of NSP was higher with high DF (P < 0.001) but decreased from the distal small intestine to the mid colon (P < 0.001).
33231591	3	132	theme	polysaccharides	857:871	arg1	content					835:841	the content	831:841	the content of non-starch polysaccharides (NSP) in all intestinal segments	831:904	High DF content increased (P < 0.001) while high protein content decreased (P = 0.004) the content of non-starch polysaccharides (NSP) in all intestinal segments.
33231591	5	133	theme	High	1058:1061	arg1	DF					1063:1064	High DF	1058:1064	High DF	1058:1064	High DF increased the relative abundance of Blautia, Faecalibacterium and Peptococcus in the caecum, the mid colon and faeces, reduced the intestinal concentrations of total short-chain fatty acids (SCFA) (P = 0.020) and acetate (P = 0.011) but slightly increased butyrate pools in the large intestine (P≤ 0.050) compared to low DF.
33231591	3	134	theme	intestinal	886:895	arg1	segments					897:904	all intestinal segments	882:904	all intestinal segments	882:904	High DF content increased (P < 0.001) while high protein content decreased (P = 0.004) the content of non-starch polysaccharides (NSP) in all intestinal segments.
33231591	0	135	from	composition	116:126	arg1	diet					156:159	a high-fat fructose-rich diet	131:159	a high-fat fructose-rich diet	131:159	Effects of dietary fibre and protein content on intestinal fibre degradation, short-chain fatty acid and microbiota composition in a high-fat fructose-rich diet induced obese Göttingen Minipig model.
32126400	5	0	dep	1D	618:619	arg1	NMR					628:630	NMR	628:630	NMR	628:630	These isolates were confirmed in the light of spectroscopic tools (containing 1D and 2D NMR), ECD spectra methods.
32126400	7	1	theme	IC50	857:860	arg1	values					862:867	IC50 values	857:867	IC50 values about 11.4-20.9 μM	857:886	Among them, compounds 3, 5, 6, 22 displayed significant effects, IC50 values about 11.4-20.9 μM.
32126400	7	1	theme	IC50	857:860	arg1	effects					848:854	significant effects	836:854	significant effects	836:854	Among them, compounds 3, 5, 6, 22 displayed significant effects, IC50 values about 11.4-20.9 μM.
32126400	2	2	theme	big	322:324	arg1	values					335:340	big economic values	322:340	big economic values	322:340	(tung tree) is an oil-producing plant with multiple uses, especially with its great potential in biodiesel production, which was a small Asian tree of the spurge family (Euphorbiaceae) with big economic values.
32126400	2	3	dep	plant	164:168	arg1	tung					133:136	tung	133:136	tung	133:136	(tung tree) is an oil-producing plant with multiple uses, especially with its great potential in biodiesel production, which was a small Asian tree of the spurge family (Euphorbiaceae) with big economic values.
32126400	4	4	theme	new	458:460	arg1	lignans					486:492	21 known lignans	477:492	21 known lignans	477:492	As a result, seven new lignans and one new coumacrin, and 21 known lignans, were isolated from the testas of V. montana.
32126400	4	4	theme	new	458:460	arg1	coumacrin					462:470	one new coumacrin	454:470	one new coumacrin	454:470	As a result, seven new lignans and one new coumacrin, and 21 known lignans, were isolated from the testas of V. montana.
32126400	4	4	theme	new	458:460	arg1	result					424:429	a result	422:429	a result	422:429	As a result, seven new lignans and one new coumacrin, and 21 known lignans, were isolated from the testas of V. montana.
32126400	4	4	theme	new	458:460	arg1	lignans					442:448	seven new lignans	432:448	seven new lignans	432:448	As a result, seven new lignans and one new coumacrin, and 21 known lignans, were isolated from the testas of V. montana.
32126400	7	5	theme	significant	836:846	arg1	values					862:867	IC50 values	857:867	IC50 values about 11.4-20.9 μM	857:886	Among them, compounds 3, 5, 6, 22 displayed significant effects, IC50 values about 11.4-20.9 μM.
32126400	7	5	theme	significant	836:846	arg1	effects					848:854	significant effects	836:854	significant effects	836:854	Among them, compounds 3, 5, 6, 22 displayed significant effects, IC50 values about 11.4-20.9 μM.
32126400	2	6	with	tree	275:278	arg1	values					335:340	big economic values	322:340	big economic values	322:340	(tung tree) is an oil-producing plant with multiple uses, especially with its great potential in biodiesel production, which was a small Asian tree of the spurge family (Euphorbiaceae) with big economic values.
32126400	6	7	theme	inhibitory	710:719	arg1	activities					721:730	their inhibitory activities	704:730	their inhibitory activities using LPS-induced BV2 cells	704:758	These isolates and crude extracts were evaluated their inhibitory activities using LPS-induced BV2 cells by assessing the NO production.
32126400	2	8	theme	Asian	269:273	arg1	production					239:248	biodiesel production	229:248	biodiesel production	229:248	(tung tree) is an oil-producing plant with multiple uses, especially with its great potential in biodiesel production, which was a small Asian tree of the spurge family (Euphorbiaceae) with big economic values.
32126400	2	8	theme	Asian	269:273	arg1	tree					275:278	a small Asian tree	261:278	a small Asian tree of the spurge family (Euphorbiaceae) with big economic values	261:340	(tung tree) is an oil-producing plant with multiple uses, especially with its great potential in biodiesel production, which was a small Asian tree of the spurge family (Euphorbiaceae) with big economic values.
32126400	2	9	dep	multiple	175:182	arg1	uses					184:187	uses	184:187	uses	184:187	(tung tree) is an oil-producing plant with multiple uses, especially with its great potential in biodiesel production, which was a small Asian tree of the spurge family (Euphorbiaceae) with big economic values.
32126400	0	10	theme	montana	100:106	arg1	testas					81:86	the testas	77:86	the testas of Vernicia montana	77:106	Structural elucidation and anti-neuroinflammatory activities of lignans from the testas of Vernicia montana.
32126400	2	11	theme	small	263:267	arg1	production					239:248	biodiesel production	229:248	biodiesel production	229:248	(tung tree) is an oil-producing plant with multiple uses, especially with its great potential in biodiesel production, which was a small Asian tree of the spurge family (Euphorbiaceae) with big economic values.
32126400	2	11	theme	small	263:267	arg1	tree					275:278	a small Asian tree	261:278	a small Asian tree of the spurge family (Euphorbiaceae) with big economic values	261:340	(tung tree) is an oil-producing plant with multiple uses, especially with its great potential in biodiesel production, which was a small Asian tree of the spurge family (Euphorbiaceae) with big economic values.
32126400	5	12	theme	ECD	634:636	arg1	spectra					638:644	ECD spectra	634:644	ECD spectra	634:644	These isolates were confirmed in the light of spectroscopic tools (containing 1D and 2D NMR), ECD spectra methods.
32126400	5	12	theme	ECD	634:636	arg1	tools					600:604	spectroscopic tools	586:604	spectroscopic tools (containing 1D and 2D NMR)	586:631	These isolates were confirmed in the light of spectroscopic tools (containing 1D and 2D NMR), ECD spectra methods.
32126400	3	13	theme	montana	398:404	arg1	compositions					379:390	small molecular compositions	363:390	small molecular compositions of V. montana	363:404	However, studies on small molecular compositions of V. montana are limited.
32126400	3	14	theme	small	363:367	arg1	compositions					379:390	small molecular compositions	363:390	small molecular compositions of V. montana	363:404	However, studies on small molecular compositions of V. montana are limited.
32126400	4	15	theme	new	438:440	arg1	lignans					486:492	21 known lignans	477:492	21 known lignans	477:492	As a result, seven new lignans and one new coumacrin, and 21 known lignans, were isolated from the testas of V. montana.
32126400	4	15	theme	new	438:440	arg1	result					424:429	a result	422:429	a result	422:429	As a result, seven new lignans and one new coumacrin, and 21 known lignans, were isolated from the testas of V. montana.
32126400	4	15	theme	new	438:440	arg1	coumacrin					462:470	one new coumacrin	454:470	one new coumacrin	454:470	As a result, seven new lignans and one new coumacrin, and 21 known lignans, were isolated from the testas of V. montana.
32126400	4	15	theme	new	438:440	arg1	lignans					442:448	seven new lignans	432:448	seven new lignans	432:448	As a result, seven new lignans and one new coumacrin, and 21 known lignans, were isolated from the testas of V. montana.
32126400	3	16	from	studies	352:358	arg1	compositions					379:390	small molecular compositions	363:390	small molecular compositions of V. montana	363:404	However, studies on small molecular compositions of V. montana are limited.
32126400	8	17	theme	potential	946:954	arg1	inhibitors					974:983	potential neuroinflammatory inhibitors	946:983	potential neuroinflammatory inhibitors	946:983	Together, the results demonstrated that lignans might be potential neuroinflammatory inhibitors.
32126400	2	18	theme	spurge	287:292	arg1	Euphorbiaceae					302:314	Euphorbiaceae	302:314	Euphorbiaceae	302:314	(tung tree) is an oil-producing plant with multiple uses, especially with its great potential in biodiesel production, which was a small Asian tree of the spurge family (Euphorbiaceae) with big economic values.
32126400	2	18	theme	spurge	287:292	arg1	family					294:299	the spurge family	283:299	the spurge family (Euphorbiaceae)	283:315	(tung tree) is an oil-producing plant with multiple uses, especially with its great potential in biodiesel production, which was a small Asian tree of the spurge family (Euphorbiaceae) with big economic values.
32126400	0	19	theme	Structural	0:9	arg1	elucidation					11:21	Structural elucidation	0:21	Structural elucidation	0:21	Structural elucidation and anti-neuroinflammatory activities of lignans from the testas of Vernicia montana.
32126400	6	20	theme	crude	674:678	arg1	extracts					680:687	crude extracts	674:687	crude extracts	674:687	These isolates and crude extracts were evaluated their inhibitory activities using LPS-induced BV2 cells by assessing the NO production.
32126400	0	21	theme	anti-neuroinflammatory	27:48	arg1	activities					50:59	anti-neuroinflammatory activities	27:59	anti-neuroinflammatory activities	27:59	Structural elucidation and anti-neuroinflammatory activities of lignans from the testas of Vernicia montana.
32126400	4	22	theme	known	480:484	arg1	lignans					486:492	21 known lignans	477:492	21 known lignans	477:492	As a result, seven new lignans and one new coumacrin, and 21 known lignans, were isolated from the testas of V. montana.
32126400	4	22	theme	known	480:484	arg1	result					424:429	a result	422:429	a result	422:429	As a result, seven new lignans and one new coumacrin, and 21 known lignans, were isolated from the testas of V. montana.
32126400	4	22	theme	known	480:484	arg1	coumacrin					462:470	one new coumacrin	454:470	one new coumacrin	454:470	As a result, seven new lignans and one new coumacrin, and 21 known lignans, were isolated from the testas of V. montana.
32126400	4	22	theme	known	480:484	arg1	lignans					442:448	seven new lignans	432:448	seven new lignans	432:448	As a result, seven new lignans and one new coumacrin, and 21 known lignans, were isolated from the testas of V. montana.
32126400	8	23	theme	neuroinflammatory	956:972	arg1	inhibitors					974:983	potential neuroinflammatory inhibitors	946:983	potential neuroinflammatory inhibitors	946:983	Together, the results demonstrated that lignans might be potential neuroinflammatory inhibitors.
32126400	6	24	theme	NO	777:778	arg1	production					780:789	the NO production	773:789	the NO production	773:789	These isolates and crude extracts were evaluated their inhibitory activities using LPS-induced BV2 cells by assessing the NO production.
32126400	3	25	theme	molecular	369:377	arg1	compositions					379:390	small molecular compositions	363:390	small molecular compositions of V. montana	363:404	However, studies on small molecular compositions of V. montana are limited.
32126400	0	26	from	testas	81:86	arg1	elucidation					11:21	Structural elucidation	0:21	Structural elucidation	0:21	Structural elucidation and anti-neuroinflammatory activities of lignans from the testas of Vernicia montana.
32126400	0	26	from	testas	81:86	arg1	activities					50:59	anti-neuroinflammatory activities	27:59	anti-neuroinflammatory activities	27:59	Structural elucidation and anti-neuroinflammatory activities of lignans from the testas of Vernicia montana.
32126400	5	27	dep	tools	600:604	arg1	methods					646:652	methods	646:652	methods	646:652	These isolates were confirmed in the light of spectroscopic tools (containing 1D and 2D NMR), ECD spectra methods.
32126400	5	28	theme	spectroscopic	586:598	arg1	spectra					638:644	ECD spectra	634:644	ECD spectra	634:644	These isolates were confirmed in the light of spectroscopic tools (containing 1D and 2D NMR), ECD spectra methods.
32126400	5	28	theme	spectroscopic	586:598	arg1	tools					600:604	spectroscopic tools	586:604	spectroscopic tools (containing 1D and 2D NMR)	586:631	These isolates were confirmed in the light of spectroscopic tools (containing 1D and 2D NMR), ECD spectra methods.
32126400	2	29	theme	oil-producing	150:162	arg1	plant					164:168	an oil-producing plant	147:168	an oil-producing plant with multiple uses, especially with its great potential in biodiesel production, which was a small Asian tree of the spurge family (Euphorbiaceae) with big economic values	147:340	(tung tree) is an oil-producing plant with multiple uses, especially with its great potential in biodiesel production, which was a small Asian tree of the spurge family (Euphorbiaceae) with big economic values.
32126400	2	30	theme	great	210:214	arg1	potential					216:224	its great potential	206:224	its great potential	206:224	(tung tree) is an oil-producing plant with multiple uses, especially with its great potential in biodiesel production, which was a small Asian tree of the spurge family (Euphorbiaceae) with big economic values.
32126400	2	31	with	plant	164:168	arg1	multiple					175:182	multiple	175:182	multiple	175:182	(tung tree) is an oil-producing plant with multiple uses, especially with its great potential in biodiesel production, which was a small Asian tree of the spurge family (Euphorbiaceae) with big economic values.
32126400	0	32	theme	lignans	64:70	arg1	elucidation					11:21	Structural elucidation	0:21	Structural elucidation	0:21	Structural elucidation and anti-neuroinflammatory activities of lignans from the testas of Vernicia montana.
32126400	0	32	theme	lignans	64:70	arg1	activities					50:59	anti-neuroinflammatory activities	27:59	anti-neuroinflammatory activities	27:59	Structural elucidation and anti-neuroinflammatory activities of lignans from the testas of Vernicia montana.
32126400	5	33	theme	tools	600:604	arg1	light					577:581	the light	573:581	the light of spectroscopic tools (containing 1D and 2D NMR), ECD spectra methods	573:652	These isolates were confirmed in the light of spectroscopic tools (containing 1D and 2D NMR), ECD spectra methods.
32126400	6	34	theme	BV2	750:752	arg1	cells					754:758	LPS-induced BV2 cells	738:758	LPS-induced BV2 cells	738:758	These isolates and crude extracts were evaluated their inhibitory activities using LPS-induced BV2 cells by assessing the NO production.
32126400	4	35	attach	isolated	500:507	arg2	coumacrin					462:470	one new coumacrin	454:470	one new coumacrin	454:470	As a result, seven new lignans and one new coumacrin, and 21 known lignans, were isolated from the testas of V. montana.
32126400	4	35	attach	isolated	500:507	arg2	lignans					486:492	21 known lignans	477:492	21 known lignans	477:492	As a result, seven new lignans and one new coumacrin, and 21 known lignans, were isolated from the testas of V. montana.
32126400	4	35	attach	isolated	500:507	arg1	testas					518:523	the testas	514:523	the testas of V. montana	514:537	As a result, seven new lignans and one new coumacrin, and 21 known lignans, were isolated from the testas of V. montana.
32126400	4	35	attach	isolated	500:507	arg2	lignans					442:448	seven new lignans	432:448	seven new lignans	432:448	As a result, seven new lignans and one new coumacrin, and 21 known lignans, were isolated from the testas of V. montana.
32126400	4	35	attach	isolated	500:507	arg2	result					424:429	a result	422:429	a result	422:429	As a result, seven new lignans and one new coumacrin, and 21 known lignans, were isolated from the testas of V. montana.
32126400	2	36	with	multiple	175:182	arg1	potential					216:224	its great potential	206:224	its great potential	206:224	(tung tree) is an oil-producing plant with multiple uses, especially with its great potential in biodiesel production, which was a small Asian tree of the spurge family (Euphorbiaceae) with big economic values.
32126400	6	37	theme	LPS-induced	738:748	arg1	cells					754:758	LPS-induced BV2 cells	738:758	LPS-induced BV2 cells	738:758	These isolates and crude extracts were evaluated their inhibitory activities using LPS-induced BV2 cells by assessing the NO production.
32126400	2	38	theme	family	294:299	arg1	production					239:248	biodiesel production	229:248	biodiesel production	229:248	(tung tree) is an oil-producing plant with multiple uses, especially with its great potential in biodiesel production, which was a small Asian tree of the spurge family (Euphorbiaceae) with big economic values.
32126400	2	38	theme	family	294:299	arg1	tree					275:278	a small Asian tree	261:278	a small Asian tree of the spurge family (Euphorbiaceae) with big economic values	261:340	(tung tree) is an oil-producing plant with multiple uses, especially with its great potential in biodiesel production, which was a small Asian tree of the spurge family (Euphorbiaceae) with big economic values.
32126400	2	39	from	plant	164:168	arg1	production					239:248	biodiesel production	229:248	biodiesel production	229:248	(tung tree) is an oil-producing plant with multiple uses, especially with its great potential in biodiesel production, which was a small Asian tree of the spurge family (Euphorbiaceae) with big economic values.
32126400	2	39	from	plant	164:168	arg1	tree					275:278	a small Asian tree	261:278	a small Asian tree of the spurge family (Euphorbiaceae) with big economic values	261:340	(tung tree) is an oil-producing plant with multiple uses, especially with its great potential in biodiesel production, which was a small Asian tree of the spurge family (Euphorbiaceae) with big economic values.
32126400	1	40	dep	Vernicia	109:116	arg1	montana					118:124	Vernicia montana	109:124	Vernicia montana Lour.	109:130	Vernicia montana Lour.
32126400	2	41	theme	biodiesel	229:237	arg1	production					239:248	biodiesel production	229:248	biodiesel production	229:248	(tung tree) is an oil-producing plant with multiple uses, especially with its great potential in biodiesel production, which was a small Asian tree of the spurge family (Euphorbiaceae) with big economic values.
32126400	2	41	theme	biodiesel	229:237	arg1	tree					275:278	a small Asian tree	261:278	a small Asian tree of the spurge family (Euphorbiaceae) with big economic values	261:340	(tung tree) is an oil-producing plant with multiple uses, especially with its great potential in biodiesel production, which was a small Asian tree of the spurge family (Euphorbiaceae) with big economic values.
32126400	2	42	theme	economic	326:333	arg1	values					335:340	big economic values	322:340	big economic values	322:340	(tung tree) is an oil-producing plant with multiple uses, especially with its great potential in biodiesel production, which was a small Asian tree of the spurge family (Euphorbiaceae) with big economic values.
32126400	4	43	theme	montana	531:537	arg1	testas					518:523	the testas	514:523	the testas of V. montana	514:537	As a result, seven new lignans and one new coumacrin, and 21 known lignans, were isolated from the testas of V. montana.
34083552	8	0	theme	higher	1192:1197	arg1	%					1190:1190	25%	1188:1190	25% higher	1188:1197	In addition, the biomass and growth rate were 57% and 25% higher, respectively, in SO120G than in the wild type, likely owing to an improved maximum quantum efficiency (Fv/Fm) of photosynthesis.
34083552	12	1	theme	wild	1961:1964	arg1	strain					1966:1971	the wild strain	1957:1971	the wild strain	1957:1971	The expression of the gene involved in the fatty acid biosynthesis and photosynthesis of SO120G was upregulated, while that related to starch metabolism decreased compared with that of the wild strain.
34083552	8	2	from	SO120G	1217:1222	arg1	type					1241:1244	the wild type	1232:1244	the wild type	1232:1244	In addition, the biomass and growth rate were 57% and 25% higher, respectively, in SO120G than in the wild type, likely owing to an improved maximum quantum efficiency (Fv/Fm) of photosynthesis.
34083552	8	2	from	SO120G	1217:1222	arg1	addition					1137:1144	addition	1137:1144	addition	1137:1144	In addition, the biomass and growth rate were 57% and 25% higher, respectively, in SO120G than in the wild type, likely owing to an improved maximum quantum efficiency (Fv/Fm) of photosynthesis.
34083552	14	3	theme	lipid	2159:2163	arg1	production					2165:2174	enhanced lipid production	2150:2174	enhanced lipid production	2150:2174	In addition, the mutant SO120G was shown to be a potential algal strain for enhanced lipid production.
34083552	0	4	with	mutant	79:84	arg1	efficiency					115:124	enhanced photosynthesis efficiency	91:124	enhanced photosynthesis efficiency	91:124	Characterization and RNA-seq transcriptomic analysis of a Scenedesmus obliqnus mutant with enhanced photosynthesis efficiency and lipid productivity.
34083552	0	4	with	mutant	79:84	arg1	productivity					136:147	lipid productivity	130:147	lipid productivity	130:147	Characterization and RNA-seq transcriptomic analysis of a Scenedesmus obliqnus mutant with enhanced photosynthesis efficiency and lipid productivity.
34083552	6	5	theme	Scenedesmus	884:894	arg1	SO120G					912:917	Scenedesmus obliqnus mutant SO120G	884:917	Scenedesmus obliqnus mutant SO120G	884:917	This study provided the photosynthetic efficiency and transcriptome profiling of a unicellular microalgae, Scenedesmus obliqnus mutant SO120G, with enhanced lipid production induced by heavy carbon ion beams.
34083552	6	5	theme	Scenedesmus	884:894	arg1	microalgae					872:881	a unicellular microalgae	858:881	a unicellular microalgae	858:881	This study provided the photosynthetic efficiency and transcriptome profiling of a unicellular microalgae, Scenedesmus obliqnus mutant SO120G, with enhanced lipid production induced by heavy carbon ion beams.
34083552	1	6	theme	next-generation	210:224	arg1	attention					187:195	significant attention	175:195	significant attention	175:195	Microalgae have received significant attention as potential next-generation microbiologic cell factories for biofuels.
34083552	1	6	theme	next-generation	210:224	arg1	factories					245:253	potential next-generation microbiologic cell factories	200:253	potential next-generation microbiologic cell factories for biofuels	200:266	Microalgae have received significant attention as potential next-generation microbiologic cell factories for biofuels.
34083552	6	7	theme	ion	975:977	arg1	beams					979:983	heavy carbon ion beams	962:983	heavy carbon ion beams	962:983	This study provided the photosynthetic efficiency and transcriptome profiling of a unicellular microalgae, Scenedesmus obliqnus mutant SO120G, with enhanced lipid production induced by heavy carbon ion beams.
34083552	15	8	theme	possible	2245:2252	arg1	genes					2254:2258	the possible genes	2241:2258	the possible genes responsible for lipid biosynthesis and photosynthesis	2241:2312	Transcriptome sequencing and annotation of the mutant suggested the possible genes responsible for lipid biosynthesis and photosynthesis, and identified the putative target genes for future genetic manipulation and biotechnological applications.
34083552	8	9	theme	improved	1266:1273	arg1	Fv/Fm					1303:1307	Fv/Fm	1303:1307	Fv/Fm	1303:1307	In addition, the biomass and growth rate were 57% and 25% higher, respectively, in SO120G than in the wild type, likely owing to an improved maximum quantum efficiency (Fv/Fm) of photosynthesis.
34083552	8	9	theme	improved	1266:1273	arg1	efficiency					1291:1300	an improved maximum quantum efficiency	1263:1300	an improved maximum quantum efficiency (Fv/Fm) of photosynthesis	1263:1326	In addition, the biomass and growth rate were 57% and 25% higher, respectively, in SO120G than in the wild type, likely owing to an improved maximum quantum efficiency (Fv/Fm) of photosynthesis.
34083552	10	10	theme	expression	1583:1592	arg1	DEGs					1601:1604	differential expression genes (DEGs)	1570:1605	differential expression genes (DEGs)	1570:1605	The transcriptome data confirmed that a total of 2077 genes with a change of at least twofold were recognized as differential expression genes (DEGs), of which 1060 genes were up-regulated and 1017 genes were down-regulated.
34083552	10	10	theme	expression	1583:1592	arg1	total					1497:1501	a total	1495:1501	a total of 2077 genes with a change of at least twofold	1495:1549	The transcriptome data confirmed that a total of 2077 genes with a change of at least twofold were recognized as differential expression genes (DEGs), of which 1060 genes were up-regulated and 1017 genes were down-regulated.
34083552	5	11	theme	lipid	707:711	arg1	yield					713:717	lipid yield	707:717	lipid yield	707:717	The deep elucidation of photosynthesis and the fatty acid metabolism pathway involved in lipid yield is valuable information for further optimization studies.
34083552	6	12	theme	microalgae	872:881	arg1	efficiency					816:825	photosynthetic efficiency	801:825	photosynthetic efficiency	801:825	This study provided the photosynthetic efficiency and transcriptome profiling of a unicellular microalgae, Scenedesmus obliqnus mutant SO120G, with enhanced lipid production induced by heavy carbon ion beams.
34083552	6	12	theme	microalgae	872:881	arg1	profiling					845:853	transcriptome profiling	831:853	transcriptome profiling	831:853	This study provided the photosynthetic efficiency and transcriptome profiling of a unicellular microalgae, Scenedesmus obliqnus mutant SO120G, with enhanced lipid production induced by heavy carbon ion beams.
34083552	1	13	theme	cell	240:243	arg1	attention					187:195	significant attention	175:195	significant attention	175:195	Microalgae have received significant attention as potential next-generation microbiologic cell factories for biofuels.
34083552	1	13	theme	cell	240:243	arg1	factories					245:253	potential next-generation microbiologic cell factories	200:253	potential next-generation microbiologic cell factories for biofuels	200:266	Microalgae have received significant attention as potential next-generation microbiologic cell factories for biofuels.
34083552	8	14	theme	quantum	1283:1289	arg1	Fv/Fm					1303:1307	Fv/Fm	1303:1307	Fv/Fm	1303:1307	In addition, the biomass and growth rate were 57% and 25% higher, respectively, in SO120G than in the wild type, likely owing to an improved maximum quantum efficiency (Fv/Fm) of photosynthesis.
34083552	8	14	theme	quantum	1283:1289	arg1	efficiency					1291:1300	an improved maximum quantum efficiency	1263:1300	an improved maximum quantum efficiency (Fv/Fm) of photosynthesis	1263:1326	In addition, the biomass and growth rate were 57% and 25% higher, respectively, in SO120G than in the wild type, likely owing to an improved maximum quantum efficiency (Fv/Fm) of photosynthesis.
34083552	11	15	theme	mutant	1757:1762	arg1	SO120G					1764:1769	the mutant SO120G	1753:1769	the mutant SO120G	1753:1769	Most of the DEGs involved in lipid biosynthesis were up-regulated with the mutant SO120G.
34083552	15	16	theme	future	2360:2365	arg1	manipulation					2375:2386	future genetic manipulation	2360:2386	future genetic manipulation	2360:2386	Transcriptome sequencing and annotation of the mutant suggested the possible genes responsible for lipid biosynthesis and photosynthesis, and identified the putative target genes for future genetic manipulation and biotechnological applications.
34083552	12	17	theme	starch	1907:1912	arg1	metabolism					1914:1923	starch metabolism	1907:1923	starch metabolism	1907:1923	The expression of the gene involved in the fatty acid biosynthesis and photosynthesis of SO120G was upregulated, while that related to starch metabolism decreased compared with that of the wild strain.
34083552	4	18	dep	pathways	535:542	arg1	the					531:533	the	531:533	the	531:533	An RNA-seq technology is used to identify the pathways and genes of importance related to photosynthesis and biofuel production.
34083552	5	19	theme	further	747:753	arg1	studies					768:774	further optimization studies	747:774	further optimization studies	747:774	The deep elucidation of photosynthesis and the fatty acid metabolism pathway involved in lipid yield is valuable information for further optimization studies.
34083552	15	20	theme	putative	2334:2341	arg1	genes					2350:2354	the putative target genes	2330:2354	the putative target genes for future genetic manipulation and biotechnological applications	2330:2420	Transcriptome sequencing and annotation of the mutant suggested the possible genes responsible for lipid biosynthesis and photosynthesis, and identified the putative target genes for future genetic manipulation and biotechnological applications.
34083552	8	21	theme	photosynthesis	1313:1326	arg1	Fv/Fm					1303:1307	Fv/Fm	1303:1307	Fv/Fm	1303:1307	In addition, the biomass and growth rate were 57% and 25% higher, respectively, in SO120G than in the wild type, likely owing to an improved maximum quantum efficiency (Fv/Fm) of photosynthesis.
34083552	8	21	theme	photosynthesis	1313:1326	arg1	efficiency					1291:1300	an improved maximum quantum efficiency	1263:1300	an improved maximum quantum efficiency (Fv/Fm) of photosynthesis	1263:1326	In addition, the biomass and growth rate were 57% and 25% higher, respectively, in SO120G than in the wild type, likely owing to an improved maximum quantum efficiency (Fv/Fm) of photosynthesis.
34083552	11	22	theme	lipid	1711:1715	arg1	biosynthesis					1717:1728	lipid biosynthesis	1711:1728	lipid biosynthesis	1711:1728	Most of the DEGs involved in lipid biosynthesis were up-regulated with the mutant SO120G.
34083552	7	23	theme	nitrogen	1104:1111	arg1	condition					1123:1131	the nitrogen deficient condition	1100:1131	the nitrogen deficient condition	1100:1131	The lipid yield (52.5 mg L-1) of SO120G mutant were enhanced 2.4 fold compared with that of the wild strain under the nitrogen deficient condition.
34083552	2	24	theme	biofuels	307:314	arg1	cost-effective					340:353	cost-effective	340:353	cost-effective	340:353	However, the production of microalgal biofuels is not yet sufficiently cost-effective for commercial applications.
34083552	2	24	theme	biofuels	307:314	arg1	production					282:291	the production	278:291	the production of microalgal biofuels	278:314	However, the production of microalgal biofuels is not yet sufficiently cost-effective for commercial applications.
34083552	15	25	theme	Transcriptome	2177:2189	arg1	sequencing					2191:2200	Transcriptome sequencing	2177:2200	Transcriptome sequencing	2177:2200	Transcriptome sequencing and annotation of the mutant suggested the possible genes responsible for lipid biosynthesis and photosynthesis, and identified the putative target genes for future genetic manipulation and biotechnological applications.
34083552	3	26	theme	higher	394:399	arg1	strains					417:423	higher lipid-producing strains	394:423	higher lipid-producing strains	394:423	To screen higher lipid-producing strains, heavy carbon ion beams are applied to induce a genetic mutant.
34083552	7	27	theme	wild	1082:1085	arg1	strain					1087:1092	the wild strain	1078:1092	the wild strain under the nitrogen deficient condition	1078:1131	The lipid yield (52.5 mg L-1) of SO120G mutant were enhanced 2.4 fold compared with that of the wild strain under the nitrogen deficient condition.
34083552	0	28	theme	obliqnus	70:77	arg1	mutant					79:84	a Scenedesmus obliqnus mutant	56:84	a Scenedesmus obliqnus mutant with enhanced photosynthesis efficiency and lipid productivity	56:147	Characterization and RNA-seq transcriptomic analysis of a Scenedesmus obliqnus mutant with enhanced photosynthesis efficiency and lipid productivity.
34083552	13	29	theme	promising	2030:2038	arg1	strategy					2040:2047	an promising strategy	2027:2047	an promising strategy for quality improvement	2027:2071	This work demonstrated that heavy-ion irradiation is an promising strategy for quality improvement.
34083552	13	29	theme	promising	2030:2038	arg1	irradiation					2012:2022	heavy-ion irradiation	2002:2022	heavy-ion irradiation	2002:2022	This work demonstrated that heavy-ion irradiation is an promising strategy for quality improvement.
34083552	1	30	theme	significant	175:185	arg1	attention					187:195	significant attention	175:195	significant attention	175:195	Microalgae have received significant attention as potential next-generation microbiologic cell factories for biofuels.
34083552	1	30	theme	significant	175:185	arg1	factories					245:253	potential next-generation microbiologic cell factories	200:253	potential next-generation microbiologic cell factories for biofuels	200:266	Microalgae have received significant attention as potential next-generation microbiologic cell factories for biofuels.
34083552	3	31	theme	heavy	426:430	arg1	beams					443:447	heavy carbon ion beams	426:447	heavy carbon ion beams	426:447	To screen higher lipid-producing strains, heavy carbon ion beams are applied to induce a genetic mutant.
34083552	4	32	theme	importance	557:566	arg1	pathways					535:542	pathways	535:542	pathways	535:542	An RNA-seq technology is used to identify the pathways and genes of importance related to photosynthesis and biofuel production.
34083552	4	32	theme	importance	557:566	arg1	genes					548:552	genes	548:552	genes	548:552	An RNA-seq technology is used to identify the pathways and genes of importance related to photosynthesis and biofuel production.
34083552	6	33	theme	mutant	905:910	arg1	SO120G					912:917	Scenedesmus obliqnus mutant SO120G	884:917	Scenedesmus obliqnus mutant SO120G	884:917	This study provided the photosynthetic efficiency and transcriptome profiling of a unicellular microalgae, Scenedesmus obliqnus mutant SO120G, with enhanced lipid production induced by heavy carbon ion beams.
34083552	6	33	theme	mutant	905:910	arg1	microalgae					872:881	a unicellular microalgae	858:881	a unicellular microalgae	858:881	This study provided the photosynthetic efficiency and transcriptome profiling of a unicellular microalgae, Scenedesmus obliqnus mutant SO120G, with enhanced lipid production induced by heavy carbon ion beams.
34083552	0	34	theme	photosynthesis	100:113	arg1	efficiency					115:124	enhanced photosynthesis efficiency	91:124	enhanced photosynthesis efficiency	91:124	Characterization and RNA-seq transcriptomic analysis of a Scenedesmus obliqnus mutant with enhanced photosynthesis efficiency and lipid productivity.
34083552	13	35	theme	heavy-ion	2002:2010	arg1	strategy					2040:2047	an promising strategy	2027:2047	an promising strategy for quality improvement	2027:2071	This work demonstrated that heavy-ion irradiation is an promising strategy for quality improvement.
34083552	13	35	theme	heavy-ion	2002:2010	arg1	irradiation					2012:2022	heavy-ion irradiation	2002:2022	heavy-ion irradiation	2002:2022	This work demonstrated that heavy-ion irradiation is an promising strategy for quality improvement.
34083552	5	36	theme	acid	671:674	arg1	pathway					687:693	the fatty acid metabolism pathway	661:693	the fatty acid metabolism pathway involved in lipid yield	661:717	The deep elucidation of photosynthesis and the fatty acid metabolism pathway involved in lipid yield is valuable information for further optimization studies.
34083552	7	37	theme	SO120G	1019:1024	arg1	mutant					1026:1031	SO120G mutant	1019:1031	SO120G mutant	1019:1031	The lipid yield (52.5 mg L-1) of SO120G mutant were enhanced 2.4 fold compared with that of the wild strain under the nitrogen deficient condition.
34083552	12	38	dep	biosynthesis	1826:1837	arg1	the					1811:1813	the	1811:1813	the	1811:1813	The expression of the gene involved in the fatty acid biosynthesis and photosynthesis of SO120G was upregulated, while that related to starch metabolism decreased compared with that of the wild strain.
34083552	0	39	theme	mutant	79:84	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and RNA-seq transcriptomic analysis of a Scenedesmus obliqnus mutant with enhanced photosynthesis efficiency and lipid productivity.
34083552	0	39	theme	mutant	79:84	arg1	analysis					44:51	RNA-seq transcriptomic analysis	21:51	RNA-seq transcriptomic analysis	21:51	Characterization and RNA-seq transcriptomic analysis of a Scenedesmus obliqnus mutant with enhanced photosynthesis efficiency and lipid productivity.
34083552	9	40	theme	major	1340:1344	arg1	compositions					1354:1365	the major pigment compositions	1336:1365	the major pigment compositions	1336:1365	As for the major pigment compositions, the content of chlorophyll a and carotenoids was higher in SO120G than in the wild type.
34083552	5	41	theme	photosynthesis	642:655	arg1	elucidation					627:637	The deep elucidation	618:637	The deep elucidation of photosynthesis and the fatty acid metabolism pathway involved in lipid yield	618:717	The deep elucidation of photosynthesis and the fatty acid metabolism pathway involved in lipid yield is valuable information for further optimization studies.
34083552	5	41	theme	photosynthesis	642:655	arg1	information					731:741	valuable information	722:741	valuable information for further optimization studies	722:774	The deep elucidation of photosynthesis and the fatty acid metabolism pathway involved in lipid yield is valuable information for further optimization studies.
34083552	8	42	from	%	1190:1190	arg1	type					1241:1244	the wild type	1232:1244	the wild type	1232:1244	In addition, the biomass and growth rate were 57% and 25% higher, respectively, in SO120G than in the wild type, likely owing to an improved maximum quantum efficiency (Fv/Fm) of photosynthesis.
34083552	8	42	from	%	1190:1190	arg1	addition					1137:1144	addition	1137:1144	addition	1137:1144	In addition, the biomass and growth rate were 57% and 25% higher, respectively, in SO120G than in the wild type, likely owing to an improved maximum quantum efficiency (Fv/Fm) of photosynthesis.
34083552	3	43	theme	ion	439:441	arg1	beams					443:447	heavy carbon ion beams	426:447	heavy carbon ion beams	426:447	To screen higher lipid-producing strains, heavy carbon ion beams are applied to induce a genetic mutant.
34083552	0	44	theme	RNA-seq	21:27	arg1	analysis					44:51	RNA-seq transcriptomic analysis	21:51	RNA-seq transcriptomic analysis	21:51	Characterization and RNA-seq transcriptomic analysis of a Scenedesmus obliqnus mutant with enhanced photosynthesis efficiency and lipid productivity.
34083552	12	45	theme	acid	1821:1824	arg1	biosynthesis					1826:1837	fatty acid biosynthesis	1815:1837	fatty acid biosynthesis	1815:1837	The expression of the gene involved in the fatty acid biosynthesis and photosynthesis of SO120G was upregulated, while that related to starch metabolism decreased compared with that of the wild strain.
34083552	6	46	theme	carbon	968:973	arg1	beams					979:983	heavy carbon ion beams	962:983	heavy carbon ion beams	962:983	This study provided the photosynthetic efficiency and transcriptome profiling of a unicellular microalgae, Scenedesmus obliqnus mutant SO120G, with enhanced lipid production induced by heavy carbon ion beams.
34083552	12	47	theme	gene	1794:1797	arg1	expression					1776:1785	The expression	1772:1785	The expression of the gene involved in the fatty acid biosynthesis and photosynthesis of SO120G	1772:1866	The expression of the gene involved in the fatty acid biosynthesis and photosynthesis of SO120G was upregulated, while that related to starch metabolism decreased compared with that of the wild strain.
34083552	6	48	theme	transcriptome	831:843	arg1	profiling					845:853	transcriptome profiling	831:853	transcriptome profiling	831:853	This study provided the photosynthetic efficiency and transcriptome profiling of a unicellular microalgae, Scenedesmus obliqnus mutant SO120G, with enhanced lipid production induced by heavy carbon ion beams.
34083552	9	49	theme	a	1395:1395	arg1	content					1372:1378	the content	1368:1378	the content of chlorophyll a and carotenoids	1368:1411	As for the major pigment compositions, the content of chlorophyll a and carotenoids was higher in SO120G than in the wild type.
34083552	9	49	theme	a	1395:1395	arg1	higher					1417:1422	higher	1417:1422	higher	1417:1422	As for the major pigment compositions, the content of chlorophyll a and carotenoids was higher in SO120G than in the wild type.
34083552	6	50	theme	enhanced	925:932	arg1	production					940:949	enhanced lipid production	925:949	enhanced lipid production induced by heavy carbon ion beams	925:983	This study provided the photosynthetic efficiency and transcriptome profiling of a unicellular microalgae, Scenedesmus obliqnus mutant SO120G, with enhanced lipid production induced by heavy carbon ion beams.
34083552	9	51	theme	carotenoids	1401:1411	arg1	content					1372:1378	the content	1368:1378	the content of chlorophyll a and carotenoids	1368:1411	As for the major pigment compositions, the content of chlorophyll a and carotenoids was higher in SO120G than in the wild type.
34083552	9	51	theme	carotenoids	1401:1411	arg1	higher					1417:1422	higher	1417:1422	higher	1417:1422	As for the major pigment compositions, the content of chlorophyll a and carotenoids was higher in SO120G than in the wild type.
34083552	15	52	theme	lipid	2276:2280	arg1	biosynthesis					2282:2293	lipid biosynthesis	2276:2293	lipid biosynthesis	2276:2293	Transcriptome sequencing and annotation of the mutant suggested the possible genes responsible for lipid biosynthesis and photosynthesis, and identified the putative target genes for future genetic manipulation and biotechnological applications.
34083552	5	53	theme	valuable	722:729	arg1	elucidation					627:637	The deep elucidation	618:637	The deep elucidation of photosynthesis and the fatty acid metabolism pathway involved in lipid yield	618:717	The deep elucidation of photosynthesis and the fatty acid metabolism pathway involved in lipid yield is valuable information for further optimization studies.
34083552	5	53	theme	valuable	722:729	arg1	information					731:741	valuable information	722:741	valuable information for further optimization studies	722:774	The deep elucidation of photosynthesis and the fatty acid metabolism pathway involved in lipid yield is valuable information for further optimization studies.
34083552	15	54	theme	responsible	2260:2270	arg1	genes					2254:2258	the possible genes	2241:2258	the possible genes responsible for lipid biosynthesis and photosynthesis	2241:2312	Transcriptome sequencing and annotation of the mutant suggested the possible genes responsible for lipid biosynthesis and photosynthesis, and identified the putative target genes for future genetic manipulation and biotechnological applications.
34083552	10	55	theme	twofold	1543:1549	arg1	change					1524:1529	a change	1522:1529	a change of at least twofold	1522:1549	The transcriptome data confirmed that a total of 2077 genes with a change of at least twofold were recognized as differential expression genes (DEGs), of which 1060 genes were up-regulated and 1017 genes were down-regulated.
34083552	5	56	theme	pathway	687:693	arg1	elucidation					627:637	The deep elucidation	618:637	The deep elucidation of photosynthesis and the fatty acid metabolism pathway involved in lipid yield	618:717	The deep elucidation of photosynthesis and the fatty acid metabolism pathway involved in lipid yield is valuable information for further optimization studies.
34083552	5	56	theme	pathway	687:693	arg1	information					731:741	valuable information	722:741	valuable information for further optimization studies	722:774	The deep elucidation of photosynthesis and the fatty acid metabolism pathway involved in lipid yield is valuable information for further optimization studies.
34083552	6	57	theme	obliqnus	896:903	arg1	SO120G					912:917	Scenedesmus obliqnus mutant SO120G	884:917	Scenedesmus obliqnus mutant SO120G	884:917	This study provided the photosynthetic efficiency and transcriptome profiling of a unicellular microalgae, Scenedesmus obliqnus mutant SO120G, with enhanced lipid production induced by heavy carbon ion beams.
34083552	6	57	theme	obliqnus	896:903	arg1	microalgae					872:881	a unicellular microalgae	858:881	a unicellular microalgae	858:881	This study provided the photosynthetic efficiency and transcriptome profiling of a unicellular microalgae, Scenedesmus obliqnus mutant SO120G, with enhanced lipid production induced by heavy carbon ion beams.
34083552	9	58	theme	wild	1446:1449	arg1	type					1451:1454	the wild type	1442:1454	the wild type	1442:1454	As for the major pigment compositions, the content of chlorophyll a and carotenoids was higher in SO120G than in the wild type.
34083552	1	59	theme	potential	200:208	arg1	attention					187:195	significant attention	175:195	significant attention	175:195	Microalgae have received significant attention as potential next-generation microbiologic cell factories for biofuels.
34083552	1	59	theme	potential	200:208	arg1	factories					245:253	potential next-generation microbiologic cell factories	200:253	potential next-generation microbiologic cell factories for biofuels	200:266	Microalgae have received significant attention as potential next-generation microbiologic cell factories for biofuels.
34083552	14	60	theme	algal	2133:2137	arg1	SO120G					2098:2103	the mutant SO120G	2087:2103	the mutant SO120G	2087:2103	In addition, the mutant SO120G was shown to be a potential algal strain for enhanced lipid production.
34083552	14	60	theme	algal	2133:2137	arg1	strain					2139:2144	a potential algal strain	2121:2144	a potential algal strain for enhanced lipid production	2121:2174	In addition, the mutant SO120G was shown to be a potential algal strain for enhanced lipid production.
34083552	10	61	theme	genes	1594:1598	arg1	DEGs					1601:1604	differential expression genes (DEGs)	1570:1605	differential expression genes (DEGs)	1570:1605	The transcriptome data confirmed that a total of 2077 genes with a change of at least twofold were recognized as differential expression genes (DEGs), of which 1060 genes were up-regulated and 1017 genes were down-regulated.
34083552	10	61	theme	genes	1594:1598	arg1	total					1497:1501	a total	1495:1501	a total of 2077 genes with a change of at least twofold	1495:1549	The transcriptome data confirmed that a total of 2077 genes with a change of at least twofold were recognized as differential expression genes (DEGs), of which 1060 genes were up-regulated and 1017 genes were down-regulated.
34083552	1	62	theme	microbiologic	226:238	arg1	attention					187:195	significant attention	175:195	significant attention	175:195	Microalgae have received significant attention as potential next-generation microbiologic cell factories for biofuels.
34083552	1	62	theme	microbiologic	226:238	arg1	factories					245:253	potential next-generation microbiologic cell factories	200:253	potential next-generation microbiologic cell factories for biofuels	200:266	Microalgae have received significant attention as potential next-generation microbiologic cell factories for biofuels.
34083552	10	63	theme	differential	1570:1581	arg1	DEGs					1601:1604	differential expression genes (DEGs)	1570:1605	differential expression genes (DEGs)	1570:1605	The transcriptome data confirmed that a total of 2077 genes with a change of at least twofold were recognized as differential expression genes (DEGs), of which 1060 genes were up-regulated and 1017 genes were down-regulated.
34083552	10	63	theme	differential	1570:1581	arg1	total					1497:1501	a total	1495:1501	a total of 2077 genes with a change of at least twofold	1495:1549	The transcriptome data confirmed that a total of 2077 genes with a change of at least twofold were recognized as differential expression genes (DEGs), of which 1060 genes were up-regulated and 1017 genes were down-regulated.
34083552	6	64	theme	unicellular	860:870	arg1	SO120G					912:917	Scenedesmus obliqnus mutant SO120G	884:917	Scenedesmus obliqnus mutant SO120G	884:917	This study provided the photosynthetic efficiency and transcriptome profiling of a unicellular microalgae, Scenedesmus obliqnus mutant SO120G, with enhanced lipid production induced by heavy carbon ion beams.
34083552	6	64	theme	unicellular	860:870	arg1	microalgae					872:881	a unicellular microalgae	858:881	a unicellular microalgae	858:881	This study provided the photosynthetic efficiency and transcriptome profiling of a unicellular microalgae, Scenedesmus obliqnus mutant SO120G, with enhanced lipid production induced by heavy carbon ion beams.
34083552	2	65	theme	commercial	359:368	arg1	applications					370:381	commercial applications	359:381	commercial applications	359:381	However, the production of microalgal biofuels is not yet sufficiently cost-effective for commercial applications.
34083552	8	66	theme	maximum	1275:1281	arg1	Fv/Fm					1303:1307	Fv/Fm	1303:1307	Fv/Fm	1303:1307	In addition, the biomass and growth rate were 57% and 25% higher, respectively, in SO120G than in the wild type, likely owing to an improved maximum quantum efficiency (Fv/Fm) of photosynthesis.
34083552	8	66	theme	maximum	1275:1281	arg1	efficiency					1291:1300	an improved maximum quantum efficiency	1263:1300	an improved maximum quantum efficiency (Fv/Fm) of photosynthesis	1263:1326	In addition, the biomass and growth rate were 57% and 25% higher, respectively, in SO120G than in the wild type, likely owing to an improved maximum quantum efficiency (Fv/Fm) of photosynthesis.
34083552	6	67	with	microalgae	872:881	arg1	production					940:949	enhanced lipid production	925:949	enhanced lipid production induced by heavy carbon ion beams	925:983	This study provided the photosynthetic efficiency and transcriptome profiling of a unicellular microalgae, Scenedesmus obliqnus mutant SO120G, with enhanced lipid production induced by heavy carbon ion beams.
34083552	15	68	theme	genetic	2367:2373	arg1	manipulation					2375:2386	future genetic manipulation	2360:2386	future genetic manipulation	2360:2386	Transcriptome sequencing and annotation of the mutant suggested the possible genes responsible for lipid biosynthesis and photosynthesis, and identified the putative target genes for future genetic manipulation and biotechnological applications.
34083552	8	69	theme	wild	1236:1239	arg1	type					1241:1244	the wild type	1232:1244	the wild type	1232:1244	In addition, the biomass and growth rate were 57% and 25% higher, respectively, in SO120G than in the wild type, likely owing to an improved maximum quantum efficiency (Fv/Fm) of photosynthesis.
34083552	14	70	theme	potential	2123:2131	arg1	SO120G					2098:2103	the mutant SO120G	2087:2103	the mutant SO120G	2087:2103	In addition, the mutant SO120G was shown to be a potential algal strain for enhanced lipid production.
34083552	14	70	theme	potential	2123:2131	arg1	strain					2139:2144	a potential algal strain	2121:2144	a potential algal strain for enhanced lipid production	2121:2174	In addition, the mutant SO120G was shown to be a potential algal strain for enhanced lipid production.
34083552	7	71	theme	deficient	1113:1121	arg1	condition					1123:1131	the nitrogen deficient condition	1100:1131	the nitrogen deficient condition	1100:1131	The lipid yield (52.5 mg L-1) of SO120G mutant were enhanced 2.4 fold compared with that of the wild strain under the nitrogen deficient condition.
34083552	5	72	theme	optimization	755:766	arg1	studies					768:774	further optimization studies	747:774	further optimization studies	747:774	The deep elucidation of photosynthesis and the fatty acid metabolism pathway involved in lipid yield is valuable information for further optimization studies.
34083552	15	73	theme	target	2343:2348	arg1	genes					2350:2354	the putative target genes	2330:2354	the putative target genes for future genetic manipulation and biotechnological applications	2330:2420	Transcriptome sequencing and annotation of the mutant suggested the possible genes responsible for lipid biosynthesis and photosynthesis, and identified the putative target genes for future genetic manipulation and biotechnological applications.
34083552	5	74	theme	deep	622:625	arg1	elucidation					627:637	The deep elucidation	618:637	The deep elucidation of photosynthesis and the fatty acid metabolism pathway involved in lipid yield	618:717	The deep elucidation of photosynthesis and the fatty acid metabolism pathway involved in lipid yield is valuable information for further optimization studies.
34083552	5	74	theme	deep	622:625	arg1	information					731:741	valuable information	722:741	valuable information for further optimization studies	722:774	The deep elucidation of photosynthesis and the fatty acid metabolism pathway involved in lipid yield is valuable information for further optimization studies.
34083552	4	75	theme	RNA-seq	492:498	arg1	technology					500:509	An RNA-seq technology	489:509	An RNA-seq technology	489:509	An RNA-seq technology is used to identify the pathways and genes of importance related to photosynthesis and biofuel production.
34083552	4	76	used	used	514:517	arg2	technology					500:509	An RNA-seq technology	489:509	An RNA-seq technology	489:509	An RNA-seq technology is used to identify the pathways and genes of importance related to photosynthesis and biofuel production.
34083552	2	77	theme	microalgal	296:305	arg1	biofuels					307:314	microalgal biofuels	296:314	microalgal biofuels	296:314	However, the production of microalgal biofuels is not yet sufficiently cost-effective for commercial applications.
34083552	3	78	theme	lipid-producing	401:415	arg1	strains					417:423	higher lipid-producing strains	394:423	higher lipid-producing strains	394:423	To screen higher lipid-producing strains, heavy carbon ion beams are applied to induce a genetic mutant.
34083552	0	79	theme	enhanced	91:98	arg1	efficiency					115:124	enhanced photosynthesis efficiency	91:124	enhanced photosynthesis efficiency	91:124	Characterization and RNA-seq transcriptomic analysis of a Scenedesmus obliqnus mutant with enhanced photosynthesis efficiency and lipid productivity.
34083552	15	80	theme	biotechnological	2392:2407	arg1	applications					2409:2420	biotechnological applications	2392:2420	biotechnological applications	2392:2420	Transcriptome sequencing and annotation of the mutant suggested the possible genes responsible for lipid biosynthesis and photosynthesis, and identified the putative target genes for future genetic manipulation and biotechnological applications.
34083552	8	81	from	%	1182:1182	arg1	type					1241:1244	the wild type	1232:1244	the wild type	1232:1244	In addition, the biomass and growth rate were 57% and 25% higher, respectively, in SO120G than in the wild type, likely owing to an improved maximum quantum efficiency (Fv/Fm) of photosynthesis.
34083552	8	81	from	%	1182:1182	arg1	addition					1137:1144	addition	1137:1144	addition	1137:1144	In addition, the biomass and growth rate were 57% and 25% higher, respectively, in SO120G than in the wild type, likely owing to an improved maximum quantum efficiency (Fv/Fm) of photosynthesis.
34083552	4	82	theme	related	568:574	arg1	pathways					535:542	pathways	535:542	pathways	535:542	An RNA-seq technology is used to identify the pathways and genes of importance related to photosynthesis and biofuel production.
34083552	14	83	theme	mutant	2091:2096	arg1	strain					2139:2144	a potential algal strain	2121:2144	a potential algal strain for enhanced lipid production	2121:2174	In addition, the mutant SO120G was shown to be a potential algal strain for enhanced lipid production.
34083552	14	83	theme	mutant	2091:2096	arg1	SO120G					2098:2103	the mutant SO120G	2087:2103	the mutant SO120G	2087:2103	In addition, the mutant SO120G was shown to be a potential algal strain for enhanced lipid production.
34083552	13	84	theme	quality	2053:2059	arg1	improvement					2061:2071	quality improvement	2053:2071	quality improvement	2053:2071	This work demonstrated that heavy-ion irradiation is an promising strategy for quality improvement.
34083552	5	85	theme	fatty	665:669	arg1	pathway					687:693	the fatty acid metabolism pathway	661:693	the fatty acid metabolism pathway involved in lipid yield	661:717	The deep elucidation of photosynthesis and the fatty acid metabolism pathway involved in lipid yield is valuable information for further optimization studies.
34083552	10	86	with	genes	1511:1515	arg1	change					1524:1529	a change	1522:1529	a change of at least twofold	1522:1549	The transcriptome data confirmed that a total of 2077 genes with a change of at least twofold were recognized as differential expression genes (DEGs), of which 1060 genes were up-regulated and 1017 genes were down-regulated.
34083552	3	87	theme	carbon	432:437	arg1	beams					443:447	heavy carbon ion beams	426:447	heavy carbon ion beams	426:447	To screen higher lipid-producing strains, heavy carbon ion beams are applied to induce a genetic mutant.
34083552	0	88	theme	lipid	130:134	arg1	productivity					136:147	lipid productivity	130:147	lipid productivity	130:147	Characterization and RNA-seq transcriptomic analysis of a Scenedesmus obliqnus mutant with enhanced photosynthesis efficiency and lipid productivity.
34083552	12	89	theme	SO120G	1861:1866	arg1	biosynthesis					1826:1837	fatty acid biosynthesis	1815:1837	fatty acid biosynthesis	1815:1837	The expression of the gene involved in the fatty acid biosynthesis and photosynthesis of SO120G was upregulated, while that related to starch metabolism decreased compared with that of the wild strain.
34083552	12	89	theme	SO120G	1861:1866	arg1	photosynthesis					1843:1856	photosynthesis	1843:1856	photosynthesis	1843:1856	The expression of the gene involved in the fatty acid biosynthesis and photosynthesis of SO120G was upregulated, while that related to starch metabolism decreased compared with that of the wild strain.
34083552	5	90	theme	metabolism	676:685	arg1	pathway					687:693	the fatty acid metabolism pathway	661:693	the fatty acid metabolism pathway involved in lipid yield	661:717	The deep elucidation of photosynthesis and the fatty acid metabolism pathway involved in lipid yield is valuable information for further optimization studies.
34083552	10	91	theme	transcriptome	1461:1473	arg1	data					1475:1478	The transcriptome data	1457:1478	The transcriptome data	1457:1478	The transcriptome data confirmed that a total of 2077 genes with a change of at least twofold were recognized as differential expression genes (DEGs), of which 1060 genes were up-regulated and 1017 genes were down-regulated.
34083552	7	92	theme	mutant	1026:1031	arg1	yield					996:1000	The lipid yield	986:1000	The lipid yield (52.5 mg L-1) of SO120G mutant	986:1031	The lipid yield (52.5 mg L-1) of SO120G mutant were enhanced 2.4 fold compared with that of the wild strain under the nitrogen deficient condition.
34083552	7	92	theme	mutant	1026:1031	arg1	52.5 mg L-1					1003:1013	52.5 mg L-1	1003:1013	52.5 mg L-1	1003:1013	The lipid yield (52.5 mg L-1) of SO120G mutant were enhanced 2.4 fold compared with that of the wild strain under the nitrogen deficient condition.
34083552	0	93	theme	transcriptomic	29:42	arg1	analysis					44:51	RNA-seq transcriptomic analysis	21:51	RNA-seq transcriptomic analysis	21:51	Characterization and RNA-seq transcriptomic analysis of a Scenedesmus obliqnus mutant with enhanced photosynthesis efficiency and lipid productivity.
34083552	9	94	theme	pigment	1346:1352	arg1	compositions					1354:1365	the major pigment compositions	1336:1365	the major pigment compositions	1336:1365	As for the major pigment compositions, the content of chlorophyll a and carotenoids was higher in SO120G than in the wild type.
34083552	8	95	theme	biomass	1151:1157	arg1	rate					1170:1173	the biomass and growth rate	1147:1173	rate	1170:1173	In addition, the biomass and growth rate were 57% and 25% higher, respectively, in SO120G than in the wild type, likely owing to an improved maximum quantum efficiency (Fv/Fm) of photosynthesis.
34083552	8	95	theme	biomass	1151:1157	arg1	%					1182:1182	57%	1180:1182	57%	1180:1182	In addition, the biomass and growth rate were 57% and 25% higher, respectively, in SO120G than in the wild type, likely owing to an improved maximum quantum efficiency (Fv/Fm) of photosynthesis.
34083552	8	96	theme	growth	1163:1168	arg1	rate					1170:1173	the biomass and growth rate	1147:1173	rate	1170:1173	In addition, the biomass and growth rate were 57% and 25% higher, respectively, in SO120G than in the wild type, likely owing to an improved maximum quantum efficiency (Fv/Fm) of photosynthesis.
34083552	8	96	theme	growth	1163:1168	arg1	%					1182:1182	57%	1180:1182	57%	1180:1182	In addition, the biomass and growth rate were 57% and 25% higher, respectively, in SO120G than in the wild type, likely owing to an improved maximum quantum efficiency (Fv/Fm) of photosynthesis.
34083552	6	97	theme	heavy	962:966	arg1	beams					979:983	heavy carbon ion beams	962:983	heavy carbon ion beams	962:983	This study provided the photosynthetic efficiency and transcriptome profiling of a unicellular microalgae, Scenedesmus obliqnus mutant SO120G, with enhanced lipid production induced by heavy carbon ion beams.
34083552	3	98	theme	genetic	473:479	arg1	mutant					481:486	a genetic mutant	471:486	a genetic mutant	471:486	To screen higher lipid-producing strains, heavy carbon ion beams are applied to induce a genetic mutant.
34083552	0	99	theme	Scenedesmus	58:68	arg1	mutant					79:84	a Scenedesmus obliqnus mutant	56:84	a Scenedesmus obliqnus mutant with enhanced photosynthesis efficiency and lipid productivity	56:147	Characterization and RNA-seq transcriptomic analysis of a Scenedesmus obliqnus mutant with enhanced photosynthesis efficiency and lipid productivity.
34083552	15	100	theme	mutant	2224:2229	arg1	annotation					2206:2215	annotation	2206:2215	annotation	2206:2215	Transcriptome sequencing and annotation of the mutant suggested the possible genes responsible for lipid biosynthesis and photosynthesis, and identified the putative target genes for future genetic manipulation and biotechnological applications.
34083552	15	100	theme	mutant	2224:2229	arg1	sequencing					2191:2200	Transcriptome sequencing	2177:2200	Transcriptome sequencing	2177:2200	Transcriptome sequencing and annotation of the mutant suggested the possible genes responsible for lipid biosynthesis and photosynthesis, and identified the putative target genes for future genetic manipulation and biotechnological applications.
34083552	14	101	theme	enhanced	2150:2157	arg1	production					2165:2174	enhanced lipid production	2150:2174	enhanced lipid production	2150:2174	In addition, the mutant SO120G was shown to be a potential algal strain for enhanced lipid production.
34083552	4	102	theme	biofuel	598:604	arg1	production					606:615	biofuel production	598:615	biofuel production	598:615	An RNA-seq technology is used to identify the pathways and genes of importance related to photosynthesis and biofuel production.
34083552	10	103	theme	genes	1511:1515	arg1	DEGs					1601:1604	differential expression genes (DEGs)	1570:1605	differential expression genes (DEGs)	1570:1605	The transcriptome data confirmed that a total of 2077 genes with a change of at least twofold were recognized as differential expression genes (DEGs), of which 1060 genes were up-regulated and 1017 genes were down-regulated.
34083552	10	103	theme	genes	1511:1515	arg1	total					1497:1501	a total	1495:1501	a total of 2077 genes with a change of at least twofold	1495:1549	The transcriptome data confirmed that a total of 2077 genes with a change of at least twofold were recognized as differential expression genes (DEGs), of which 1060 genes were up-regulated and 1017 genes were down-regulated.
34083552	9	104	theme	chlorophyll	1383:1393	arg1	a					1395:1395	chlorophyll a	1383:1395	chlorophyll a	1383:1395	As for the major pigment compositions, the content of chlorophyll a and carotenoids was higher in SO120G than in the wild type.
34083552	12	105	theme	fatty	1815:1819	arg1	biosynthesis					1826:1837	fatty acid biosynthesis	1815:1837	fatty acid biosynthesis	1815:1837	The expression of the gene involved in the fatty acid biosynthesis and photosynthesis of SO120G was upregulated, while that related to starch metabolism decreased compared with that of the wild strain.
34083552	6	106	theme	lipid	934:938	arg1	production					940:949	enhanced lipid production	925:949	enhanced lipid production induced by heavy carbon ion beams	925:983	This study provided the photosynthetic efficiency and transcriptome profiling of a unicellular microalgae, Scenedesmus obliqnus mutant SO120G, with enhanced lipid production induced by heavy carbon ion beams.
34083552	7	107	theme	lipid	990:994	arg1	yield					996:1000	The lipid yield	986:1000	The lipid yield (52.5 mg L-1) of SO120G mutant	986:1031	The lipid yield (52.5 mg L-1) of SO120G mutant were enhanced 2.4 fold compared with that of the wild strain under the nitrogen deficient condition.
34083552	7	107	theme	lipid	990:994	arg1	52.5 mg L-1					1003:1013	52.5 mg L-1	1003:1013	52.5 mg L-1	1003:1013	The lipid yield (52.5 mg L-1) of SO120G mutant were enhanced 2.4 fold compared with that of the wild strain under the nitrogen deficient condition.
34083552	6	108	dep	efficiency	816:825	arg1	the					797:799	the	797:799	the	797:799	This study provided the photosynthetic efficiency and transcriptome profiling of a unicellular microalgae, Scenedesmus obliqnus mutant SO120G, with enhanced lipid production induced by heavy carbon ion beams.
34083552	6	109	theme	photosynthetic	801:814	arg1	efficiency					816:825	photosynthetic efficiency	801:825	photosynthetic efficiency	801:825	This study provided the photosynthetic efficiency and transcriptome profiling of a unicellular microalgae, Scenedesmus obliqnus mutant SO120G, with enhanced lipid production induced by heavy carbon ion beams.
34358646	7	0	theme	-treated	1230:1237	arg1	mice					1239:1242	atEc -treated mice	1225:1242	atEc -treated mice	1225:1242	The abundance of Firmicutes was significantly recovered, while that of Proteobacteria decreased in atEc -treated mice compared with that in vehicle-treated wild-type mice.
34358646	5	1	theme	Hydrogen	891:898	arg1	peroxide					900:907	Hydrogen peroxide	891:907	Hydrogen peroxide levels in colitis mice	891:930	Hydrogen peroxide levels in colitis mice were noticeably decreased when the mice were administered atEc.
34358646	6	2	theme	proinflammatory	1000:1014	arg1	levels					1025:1030	The proinflammatory cytokine levels	996:1030	The proinflammatory cytokine levels	996:1030	The proinflammatory cytokine levels were decreased and regulatory T cell numbers were increased after the administration of atEc.
34358646	0	3	theme	murine	73:78	arg1	colitis					80:86	murine colitis	73:86	murine colitis	73:86	An Escherichia coli strain with extra catalase activity protects against murine colitis by scavenging hydrogen peroxide and regulating regulatory t cell/interleukin-17 pathways.
34358646	3	4	theme	-induced	692:699	arg1	colitis					701:707	dextran sulfate sodium (DSS)-induced colitis	664:707	dextran sulfate sodium (DSS)-induced colitis	664:707	METHODS The effects of an atypical commensal Escherichia coli strain harboring an additional catalase gene (compared to typical E. coli strain) on dextran sulfate sodium (DSS)-induced colitis were explored in mice.
34358646	3	5	from	effects	529:535	arg1	colitis					701:707	dextran sulfate sodium (DSS)-induced colitis	664:707	dextran sulfate sodium (DSS)-induced colitis	664:707	METHODS The effects of an atypical commensal Escherichia coli strain harboring an additional catalase gene (compared to typical E. coli strain) on dextran sulfate sodium (DSS)-induced colitis were explored in mice.
34358646	1	6	theme	BACKGROUND	178:187	arg1	disease					208:214	BACKGROUND Inflammatory bowel disease	178:214	BACKGROUND Inflammatory bowel disease (IBD)	178:220	BACKGROUND Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress.
34358646	1	6	theme	BACKGROUND	178:187	arg1	disease					248:254	a chronic inflammatory disease	225:254	a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress	225:419	BACKGROUND Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress.
34358646	1	6	theme	BACKGROUND	178:187	arg1	IBD					217:219	IBD	217:219	IBD	217:219	BACKGROUND Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress.
34358646	10	7	theme	CONCLUSIONS	1600:1610	arg1	atEc					1612:1615	CONCLUSIONS atEc	1600:1615	CONCLUSIONS atEc	1600:1615	CONCLUSIONS atEc ameliorates colonic inflammation by controlling hydrogen peroxide levels, immune responses (including regulatory T cells and IL-17A), and microbial composition.
34358646	1	8	theme	gastrointestinal	263:278	arg1	tract					280:284	the gastrointestinal tract	259:284	the gastrointestinal tract	259:284	BACKGROUND Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress.
34358646	0	9	theme	scavenging	91:100	arg1	peroxide					111:118	scavenging hydrogen peroxide	91:118	scavenging hydrogen peroxide	91:118	An Escherichia coli strain with extra catalase activity protects against murine colitis by scavenging hydrogen peroxide and regulating regulatory t cell/interleukin-17 pathways.
34358646	1	10	theme	immune	364:369	arg1	response					371:378	immune response	364:378	immune response	364:378	BACKGROUND Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress.
34358646	8	11	theme	administered	1435:1446	arg1	atEc					1448:1451	orally administered atEc	1428:1451	orally administered atEc	1428:1451	To investigate the role of interleukin (IL)-17A in mediating the anti-inflammatory effects of the atEc, IL-17A‒knockout mice were orally administered atEc.
34358646	1	12	theme	tract	280:284	arg1	disease					248:254	a chronic inflammatory disease	225:254	a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress	225:419	BACKGROUND Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress.
34358646	1	12	theme	tract	280:284	arg1	disease					208:214	BACKGROUND Inflammatory bowel disease	178:214	BACKGROUND Inflammatory bowel disease (IBD)	178:220	BACKGROUND Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress.
34358646	4	13	dep	RESULTS	732:738	arg1	atEc					762:765	atEc	762:765	atEc	762:765	RESULTS The atypical E. coli (atEc) significantly restored body weight, reduced disease activity score, and improved histological scores in mice with colitis.
34358646	4	13	dep	RESULTS	732:738	arg1	coli					756:759	The atypical E. coli	740:759	RESULTS The atypical E. coli (atEc)	732:766	RESULTS The atypical E. coli (atEc) significantly restored body weight, reduced disease activity score, and improved histological scores in mice with colitis.
34358646	9	14	theme	wild-type	1584:1592	arg1	mice					1594:1597	wild-type mice	1584:1597	wild-type mice	1584:1597	Clinical and immune responses and microbial composition were significantly reduced in IL-17A‒knockout mice compared with those in wild-type mice.
34358646	3	15	theme	strain	579:584	arg1	effects					529:535	The effects	525:535	The effects of an atypical commensal Escherichia coli strain harboring an additional catalase gene (compared to typical E. coli strain) on dextran sulfate sodium (DSS)-induced colitis	525:707	METHODS The effects of an atypical commensal Escherichia coli strain harboring an additional catalase gene (compared to typical E. coli strain) on dextran sulfate sodium (DSS)-induced colitis were explored in mice.
34358646	9	16	theme	IL-17A‒knockout	1540:1554	arg1	mice					1556:1559	IL-17A‒knockout mice	1540:1559	IL-17A‒knockout mice	1540:1559	Clinical and immune responses and microbial composition were significantly reduced in IL-17A‒knockout mice compared with those in wild-type mice.
34358646	10	17	theme	hydrogen	1665:1672	arg1	peroxide					1674:1681	hydrogen peroxide	1665:1681	hydrogen peroxide levels	1665:1688	CONCLUSIONS atEc ameliorates colonic inflammation by controlling hydrogen peroxide levels, immune responses (including regulatory T cells and IL-17A), and microbial composition.
34358646	7	18	theme	vehicle-treated	1266:1280	arg1	mice					1292:1295	vehicle-treated wild-type mice	1266:1295	vehicle-treated wild-type mice	1266:1295	The abundance of Firmicutes was significantly recovered, while that of Proteobacteria decreased in atEc -treated mice compared with that in vehicle-treated wild-type mice.
34358646	9	19	theme	microbial	1488:1496	arg1	composition					1498:1508	microbial composition	1488:1508	microbial composition	1488:1508	Clinical and immune responses and microbial composition were significantly reduced in IL-17A‒knockout mice compared with those in wild-type mice.
34358646	3	20	theme	commensal	552:560	arg1	strain					579:584	an atypical commensal Escherichia coli strain	540:584	an atypical commensal Escherichia coli strain harboring an additional catalase gene (compared to typical E. coli strain)	540:659	METHODS The effects of an atypical commensal Escherichia coli strain harboring an additional catalase gene (compared to typical E. coli strain) on dextran sulfate sodium (DSS)-induced colitis were explored in mice.
34358646	1	21	theme	Inflammatory	189:200	arg1	disease					208:214	BACKGROUND Inflammatory bowel disease	178:214	BACKGROUND Inflammatory bowel disease (IBD)	178:220	BACKGROUND Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress.
34358646	1	21	theme	Inflammatory	189:200	arg1	disease					248:254	a chronic inflammatory disease	225:254	a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress	225:419	BACKGROUND Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress.
34358646	1	21	theme	Inflammatory	189:200	arg1	IBD					217:219	IBD	217:219	IBD	217:219	BACKGROUND Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress.
34358646	0	22	theme	hydrogen	102:109	arg1	peroxide					111:118	scavenging hydrogen peroxide	91:118	scavenging hydrogen peroxide	91:118	An Escherichia coli strain with extra catalase activity protects against murine colitis by scavenging hydrogen peroxide and regulating regulatory t cell/interleukin-17 pathways.
34358646	1	23	theme	microbial	381:389	arg1	changes					391:397	microbial changes	381:397	microbial changes	381:397	BACKGROUND Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress.
34358646	10	24	theme	immune	1691:1696	arg1	cells					1732:1736	regulatory T cells	1719:1736	regulatory T cells	1719:1736	CONCLUSIONS atEc ameliorates colonic inflammation by controlling hydrogen peroxide levels, immune responses (including regulatory T cells and IL-17A), and microbial composition.
34358646	10	24	theme	immune	1691:1696	arg1	responses					1698:1706	immune responses	1691:1706	immune responses (including regulatory T cells and IL-17A)	1691:1748	CONCLUSIONS atEc ameliorates colonic inflammation by controlling hydrogen peroxide levels, immune responses (including regulatory T cells and IL-17A), and microbial composition.
34358646	10	24	theme	immune	1691:1696	arg1	IL-17A					1742:1747	IL-17A	1742:1747	IL-17A	1742:1747	CONCLUSIONS atEc ameliorates colonic inflammation by controlling hydrogen peroxide levels, immune responses (including regulatory T cells and IL-17A), and microbial composition.
34358646	3	25	theme	Escherichia	562:572	arg1	strain					579:584	an atypical commensal Escherichia coli strain	540:584	an atypical commensal Escherichia coli strain harboring an additional catalase gene (compared to typical E. coli strain)	540:659	METHODS The effects of an atypical commensal Escherichia coli strain harboring an additional catalase gene (compared to typical E. coli strain) on dextran sulfate sodium (DSS)-induced colitis were explored in mice.
34358646	11	26	theme	IBD	1827:1829	arg1	treatment					1831:1839	IBD treatment	1827:1839	IBD treatment	1827:1839	atEc could be a novel candidate of probiotic for IBD treatment.
34358646	1	27	theme	bowel	202:206	arg1	disease					208:214	BACKGROUND Inflammatory bowel disease	178:214	BACKGROUND Inflammatory bowel disease (IBD)	178:220	BACKGROUND Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress.
34358646	1	27	theme	bowel	202:206	arg1	disease					248:254	a chronic inflammatory disease	225:254	a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress	225:419	BACKGROUND Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress.
34358646	1	27	theme	bowel	202:206	arg1	IBD					217:219	IBD	217:219	IBD	217:219	BACKGROUND Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress.
34358646	0	28	theme	Escherichia	3:13	arg1	strain					20:25	An Escherichia coli strain	0:25	An Escherichia coli strain with extra catalase activity	0:54	An Escherichia coli strain with extra catalase activity protects against murine colitis by scavenging hydrogen peroxide and regulating regulatory t cell/interleukin-17 pathways.
34358646	4	29	theme	histological	849:860	arg1	scores					862:867	histological scores	849:867	histological scores in mice with colitis	849:888	RESULTS The atypical E. coli (atEc) significantly restored body weight, reduced disease activity score, and improved histological scores in mice with colitis.
34358646	3	30	theme	additional	599:608	arg1	gene					619:622	an additional catalase gene	596:622	an additional catalase gene (compared to typical E. coli strain)	596:659	METHODS The effects of an atypical commensal Escherichia coli strain harboring an additional catalase gene (compared to typical E. coli strain) on dextran sulfate sodium (DSS)-induced colitis were explored in mice.
34358646	9	31	theme	immune	1467:1472	arg1	responses					1474:1482	Clinical and immune responses	1454:1482	Clinical and immune responses	1454:1482	Clinical and immune responses and microbial composition were significantly reduced in IL-17A‒knockout mice compared with those in wild-type mice.
34358646	10	32	theme	peroxide	1674:1681	arg1	levels					1683:1688	hydrogen peroxide levels	1665:1688	hydrogen peroxide levels	1665:1688	CONCLUSIONS atEc ameliorates colonic inflammation by controlling hydrogen peroxide levels, immune responses (including regulatory T cells and IL-17A), and microbial composition.
34358646	10	32	theme	peroxide	1674:1681	arg1	IL-17A					1742:1747	IL-17A	1742:1747	IL-17A	1742:1747	CONCLUSIONS atEc ameliorates colonic inflammation by controlling hydrogen peroxide levels, immune responses (including regulatory T cells and IL-17A), and microbial composition.
34358646	10	32	theme	peroxide	1674:1681	arg1	cells					1732:1736	regulatory T cells	1719:1736	regulatory T cells	1719:1736	CONCLUSIONS atEc ameliorates colonic inflammation by controlling hydrogen peroxide levels, immune responses (including regulatory T cells and IL-17A), and microbial composition.
34358646	5	33	from	levels	909:914	arg1	mice					927:930	colitis mice	919:930	colitis mice	919:930	Hydrogen peroxide levels in colitis mice were noticeably decreased when the mice were administered atEc.
34358646	3	34	theme	catalase	610:617	arg1	gene					619:622	an additional catalase gene	596:622	an additional catalase gene (compared to typical E. coli strain)	596:659	METHODS The effects of an atypical commensal Escherichia coli strain harboring an additional catalase gene (compared to typical E. coli strain) on dextran sulfate sodium (DSS)-induced colitis were explored in mice.
34358646	8	35	theme	anti-inflammatory	1363:1379	arg1	effects					1381:1387	the anti-inflammatory effects	1359:1387	the anti-inflammatory effects of the atEc	1359:1399	To investigate the role of interleukin (IL)-17A in mediating the anti-inflammatory effects of the atEc, IL-17A‒knockout mice were orally administered atEc.
34358646	3	36	theme	typical	637:643	arg1	strain					653:658	typical E. coli strain	637:658	typical E. coli strain	637:658	METHODS The effects of an atypical commensal Escherichia coli strain harboring an additional catalase gene (compared to typical E. coli strain) on dextran sulfate sodium (DSS)-induced colitis were explored in mice.
34358646	0	37	theme	regulating	124:133	arg1	pathways					168:175	regulating regulatory t cell/interleukin-17 pathways	124:175	regulating regulatory t cell/interleukin-17 pathways	124:175	An Escherichia coli strain with extra catalase activity protects against murine colitis by scavenging hydrogen peroxide and regulating regulatory t cell/interleukin-17 pathways.
34358646	9	38	theme	Clinical	1454:1461	arg1	responses					1474:1482	Clinical and immune responses	1454:1482	Clinical and immune responses	1454:1482	Clinical and immune responses and microbial composition were significantly reduced in IL-17A‒knockout mice compared with those in wild-type mice.
34358646	8	39	theme	atEc	1396:1399	arg1	effects					1381:1387	the anti-inflammatory effects	1359:1387	the anti-inflammatory effects of the atEc	1359:1399	To investigate the role of interleukin (IL)-17A in mediating the anti-inflammatory effects of the atEc, IL-17A‒knockout mice were orally administered atEc.
34358646	8	40	theme	IL-17A‒knockout	1402:1416	arg1	mice					1418:1421	IL-17A‒knockout mice	1402:1421	IL-17A‒knockout mice	1402:1421	To investigate the role of interleukin (IL)-17A in mediating the anti-inflammatory effects of the atEc, IL-17A‒knockout mice were orally administered atEc.
34358646	6	41	theme	cell	1064:1067	arg1	numbers					1069:1075	regulatory T cell numbers	1051:1075	regulatory T cell numbers	1051:1075	The proinflammatory cytokine levels were decreased and regulatory T cell numbers were increased after the administration of atEc.
34358646	3	42	dep	METHODS	517:523	arg1	explored					714:721	explored	714:721	were explored in mice	709:729	METHODS The effects of an atypical commensal Escherichia coli strain harboring an additional catalase gene (compared to typical E. coli strain) on dextran sulfate sodium (DSS)-induced colitis were explored in mice.
34358646	5	43	theme	peroxide	900:907	arg1	levels					909:914	Hydrogen peroxide levels	891:914	Hydrogen peroxide levels in colitis mice	891:930	Hydrogen peroxide levels in colitis mice were noticeably decreased when the mice were administered atEc.
34358646	6	44	theme	T	1062:1062	arg1	numbers					1069:1075	regulatory T cell numbers	1051:1075	regulatory T cell numbers	1051:1075	The proinflammatory cytokine levels were decreased and regulatory T cell numbers were increased after the administration of atEc.
34358646	0	45	theme	extra	32:36	arg1	activity					47:54	extra catalase activity	32:54	extra catalase activity	32:54	An Escherichia coli strain with extra catalase activity protects against murine colitis by scavenging hydrogen peroxide and regulating regulatory t cell/interleukin-17 pathways.
34358646	0	46	theme	t	146:146	arg1	pathways					168:175	regulating regulatory t cell/interleukin-17 pathways	124:175	regulating regulatory t cell/interleukin-17 pathways	124:175	An Escherichia coli strain with extra catalase activity protects against murine colitis by scavenging hydrogen peroxide and regulating regulatory t cell/interleukin-17 pathways.
34358646	6	47	theme	regulatory	1051:1060	arg1	numbers					1069:1075	regulatory T cell numbers	1051:1075	regulatory T cell numbers	1051:1075	The proinflammatory cytokine levels were decreased and regulatory T cell numbers were increased after the administration of atEc.
34358646	4	48	theme	disease	812:818	arg1	score					829:833	disease activity score	812:833	disease activity score	812:833	RESULTS The atypical E. coli (atEc) significantly restored body weight, reduced disease activity score, and improved histological scores in mice with colitis.
34358646	8	49	theme	interleukin	1325:1335	arg1	-17A					1341:1344	interleukin (IL)-17A	1325:1344	interleukin (IL)-17A	1325:1344	To investigate the role of interleukin (IL)-17A in mediating the anti-inflammatory effects of the atEc, IL-17A‒knockout mice were orally administered atEc.
34358646	2	50	theme	Microbial-targeted	422:439	arg1	therapy					441:447	Microbial-targeted therapy	422:447	Microbial-targeted therapy	422:447	Microbial-targeted therapy has emerged as an alternative to immunosuppressive therapy for IBD.
34358646	3	51	dep	gene	619:622	arg1	compared					625:632	compared	625:632	compared to typical E. coli strain	625:658	METHODS The effects of an atypical commensal Escherichia coli strain harboring an additional catalase gene (compared to typical E. coli strain) on dextran sulfate sodium (DSS)-induced colitis were explored in mice.
34358646	1	52	theme	various	320:326	arg1	factors					328:334	various factors	320:334	various factors	320:334	BACKGROUND Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress.
34358646	1	52	theme	various	320:326	arg1	stress					414:419	oxidative stress	404:419	oxidative stress	404:419	BACKGROUND Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress.
34358646	1	52	theme	various	320:326	arg1	factors					355:361	genetic factors	347:361	genetic factors	347:361	BACKGROUND Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress.
34358646	1	52	theme	various	320:326	arg1	response					371:378	immune response	364:378	immune response	364:378	BACKGROUND Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress.
34358646	1	52	theme	various	320:326	arg1	changes					391:397	microbial changes	381:397	microbial changes	381:397	BACKGROUND Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress.
34358646	0	53	theme	regulatory	135:144	arg1	pathways					168:175	regulating regulatory t cell/interleukin-17 pathways	124:175	regulating regulatory t cell/interleukin-17 pathways	124:175	An Escherichia coli strain with extra catalase activity protects against murine colitis by scavenging hydrogen peroxide and regulating regulatory t cell/interleukin-17 pathways.
34358646	1	54	theme	oxidative	404:412	arg1	stress					414:419	oxidative stress	404:419	oxidative stress	404:419	BACKGROUND Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress.
34358646	0	55	with	strain	20:25	arg1	activity					47:54	extra catalase activity	32:54	extra catalase activity	32:54	An Escherichia coli strain with extra catalase activity protects against murine colitis by scavenging hydrogen peroxide and regulating regulatory t cell/interleukin-17 pathways.
34358646	10	56	theme	T	1730:1730	arg1	cells					1732:1736	regulatory T cells	1719:1736	regulatory T cells	1719:1736	CONCLUSIONS atEc ameliorates colonic inflammation by controlling hydrogen peroxide levels, immune responses (including regulatory T cells and IL-17A), and microbial composition.
34358646	2	57	theme	immunosuppressive	482:498	arg1	therapy					500:506	immunosuppressive therapy	482:506	immunosuppressive therapy	482:506	Microbial-targeted therapy has emerged as an alternative to immunosuppressive therapy for IBD.
34358646	10	58	theme	colonic	1629:1635	arg1	inflammation					1637:1648	colonic inflammation	1629:1648	colonic inflammation	1629:1648	CONCLUSIONS atEc ameliorates colonic inflammation by controlling hydrogen peroxide levels, immune responses (including regulatory T cells and IL-17A), and microbial composition.
34358646	10	59	theme	microbial	1755:1763	arg1	composition					1765:1775	microbial composition	1755:1775	microbial composition	1755:1775	CONCLUSIONS atEc ameliorates colonic inflammation by controlling hydrogen peroxide levels, immune responses (including regulatory T cells and IL-17A), and microbial composition.
34358646	0	60	theme	catalase	38:45	arg1	activity					47:54	extra catalase activity	32:54	extra catalase activity	32:54	An Escherichia coli strain with extra catalase activity protects against murine colitis by scavenging hydrogen peroxide and regulating regulatory t cell/interleukin-17 pathways.
34358646	11	61	theme	probiotic	1813:1821	arg1	candidate					1800:1808	a novel candidate	1792:1808	a novel candidate of probiotic for IBD treatment	1792:1839	atEc could be a novel candidate of probiotic for IBD treatment.
34358646	11	61	theme	probiotic	1813:1821	arg1	atEc					1778:1781	atEc	1778:1781	atEc	1778:1781	atEc could be a novel candidate of probiotic for IBD treatment.
34358646	0	62	theme	cell/interleukin-17	148:166	arg1	pathways					168:175	regulating regulatory t cell/interleukin-17 pathways	124:175	regulating regulatory t cell/interleukin-17 pathways	124:175	An Escherichia coli strain with extra catalase activity protects against murine colitis by scavenging hydrogen peroxide and regulating regulatory t cell/interleukin-17 pathways.
34358646	4	63	theme	activity	820:827	arg1	score					829:833	disease activity score	812:833	disease activity score	812:833	RESULTS The atypical E. coli (atEc) significantly restored body weight, reduced disease activity score, and improved histological scores in mice with colitis.
34358646	7	64	theme	atEc	1225:1228	arg1	mice					1239:1242	atEc -treated mice	1225:1242	atEc -treated mice	1225:1242	The abundance of Firmicutes was significantly recovered, while that of Proteobacteria decreased in atEc -treated mice compared with that in vehicle-treated wild-type mice.
34358646	3	65	dep	Escherichia	562:572	arg1	coli					574:577	coli	574:577	coli	574:577	METHODS The effects of an atypical commensal Escherichia coli strain harboring an additional catalase gene (compared to typical E. coli strain) on dextran sulfate sodium (DSS)-induced colitis were explored in mice.
34358646	4	66	theme	body	791:794	arg1	weight					796:801	body weight	791:801	body weight	791:801	RESULTS The atypical E. coli (atEc) significantly restored body weight, reduced disease activity score, and improved histological scores in mice with colitis.
34358646	3	67	theme	coli	648:651	arg1	strain					653:658	typical E. coli strain	637:658	typical E. coli strain	637:658	METHODS The effects of an atypical commensal Escherichia coli strain harboring an additional catalase gene (compared to typical E. coli strain) on dextran sulfate sodium (DSS)-induced colitis were explored in mice.
34358646	5	68	theme	colitis	919:925	arg1	mice					927:930	colitis mice	919:930	colitis mice	919:930	Hydrogen peroxide levels in colitis mice were noticeably decreased when the mice were administered atEc.
34358646	4	69	with	mice	872:875	arg1	colitis					882:888	colitis	882:888	colitis	882:888	RESULTS The atypical E. coli (atEc) significantly restored body weight, reduced disease activity score, and improved histological scores in mice with colitis.
34358646	1	70	theme	chronic	227:233	arg1	disease					248:254	a chronic inflammatory disease	225:254	a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress	225:419	BACKGROUND Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress.
34358646	1	70	theme	chronic	227:233	arg1	disease					208:214	BACKGROUND Inflammatory bowel disease	178:214	BACKGROUND Inflammatory bowel disease (IBD)	178:220	BACKGROUND Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress.
34358646	7	71	theme	wild-type	1282:1290	arg1	mice					1292:1295	vehicle-treated wild-type mice	1266:1295	vehicle-treated wild-type mice	1266:1295	The abundance of Firmicutes was significantly recovered, while that of Proteobacteria decreased in atEc -treated mice compared with that in vehicle-treated wild-type mice.
34358646	8	72	theme	-17A	1341:1344	arg1	role					1317:1320	the role	1313:1320	the role of interleukin (IL)-17A in mediating the anti-inflammatory effects of the atEc	1313:1399	To investigate the role of interleukin (IL)-17A in mediating the anti-inflammatory effects of the atEc, IL-17A‒knockout mice were orally administered atEc.
34358646	1	73	theme	inflammatory	235:246	arg1	disease					248:254	a chronic inflammatory disease	225:254	a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress	225:419	BACKGROUND Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress.
34358646	1	73	theme	inflammatory	235:246	arg1	disease					208:214	BACKGROUND Inflammatory bowel disease	178:214	BACKGROUND Inflammatory bowel disease (IBD)	178:220	BACKGROUND Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress.
34358646	11	74	theme	novel	1794:1798	arg1	candidate					1800:1808	a novel candidate	1792:1808	a novel candidate of probiotic for IBD treatment	1792:1839	atEc could be a novel candidate of probiotic for IBD treatment.
34358646	11	74	theme	novel	1794:1798	arg1	atEc					1778:1781	atEc	1778:1781	atEc	1778:1781	atEc could be a novel candidate of probiotic for IBD treatment.
34358646	0	75	dep	Escherichia	3:13	arg1	coli					15:18	coli	15:18	coli	15:18	An Escherichia coli strain with extra catalase activity protects against murine colitis by scavenging hydrogen peroxide and regulating regulatory t cell/interleukin-17 pathways.
34358646	6	76	theme	atEc	1120:1123	arg1	administration					1102:1115	the administration	1098:1115	the administration of atEc	1098:1123	The proinflammatory cytokine levels were decreased and regulatory T cell numbers were increased after the administration of atEc.
34358646	10	77	theme	regulatory	1719:1728	arg1	cells					1732:1736	regulatory T cells	1719:1736	regulatory T cells	1719:1736	CONCLUSIONS atEc ameliorates colonic inflammation by controlling hydrogen peroxide levels, immune responses (including regulatory T cells and IL-17A), and microbial composition.
34358646	7	78	theme	Firmicutes	1143:1152	arg1	abundance					1130:1138	The abundance	1126:1138	The abundance of Firmicutes	1126:1152	The abundance of Firmicutes was significantly recovered, while that of Proteobacteria decreased in atEc -treated mice compared with that in vehicle-treated wild-type mice.
34358646	1	79	theme	genetic	347:353	arg1	factors					355:361	genetic factors	347:361	genetic factors	347:361	BACKGROUND Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress.
34358646	3	80	theme	E.	645:646	arg1	strain					653:658	typical E. coli strain	637:658	typical E. coli strain	637:658	METHODS The effects of an atypical commensal Escherichia coli strain harboring an additional catalase gene (compared to typical E. coli strain) on dextran sulfate sodium (DSS)-induced colitis were explored in mice.
34358646	3	81	theme	atypical	543:550	arg1	strain					579:584	an atypical commensal Escherichia coli strain	540:584	an atypical commensal Escherichia coli strain harboring an additional catalase gene (compared to typical E. coli strain)	540:659	METHODS The effects of an atypical commensal Escherichia coli strain harboring an additional catalase gene (compared to typical E. coli strain) on dextran sulfate sodium (DSS)-induced colitis were explored in mice.
34358646	4	82	from	scores	862:867	arg1	mice					872:875	mice	872:875	mice with colitis	872:888	RESULTS The atypical E. coli (atEc) significantly restored body weight, reduced disease activity score, and improved histological scores in mice with colitis.
34358646	6	83	theme	cytokine	1016:1023	arg1	levels					1025:1030	The proinflammatory cytokine levels	996:1030	The proinflammatory cytokine levels	996:1030	The proinflammatory cytokine levels were decreased and regulatory T cell numbers were increased after the administration of atEc.
33687943	6	0	theme	cell	846:849	arg1	sequencing					855:864	Single cell RNA sequencing	839:864	Single cell RNA sequencing	839:864	Single cell RNA sequencing, together with sera metabolomics analyses were performed to identify host responsive molecules.
33687943	10	1	theme	inflammation	1310:1321	arg1	restoration					1411:1421	systematic restoration	1400:1421	systematic restoration of aberrant host amino acids metabolism in sera	1400:1469	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	1	theme	inflammation	1310:1321	arg1	enhancement					1327:1337	enhancement	1327:1337	enhancement of cellular mitochondrial and ribosomal activities	1327:1388	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	1	theme	inflammation	1310:1321	arg1	Reduction					1286:1294	Reduction	1286:1294	Reduction of intestinal inflammation	1286:1321	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	1	theme	inflammation	1310:1321	arg1	inhibition					1476:1485	inhibition	1476:1485	inhibition of lung inflammations	1476:1507	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	1	theme	inflammation	1310:1321	arg1	mechanisms					1548:1557	the important COPD ameliorative mechanisms	1516:1557	the important COPD ameliorative mechanisms	1516:1557	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	6	2	theme	host	935:938	arg1	molecules					951:959	host responsive molecules	935:959	host responsive molecules	935:959	Single cell RNA sequencing, together with sera metabolomics analyses were performed to identify host responsive molecules.
33687943	10	3	theme	inflammations	1495:1507	arg1	restoration					1411:1421	systematic restoration	1400:1421	systematic restoration of aberrant host amino acids metabolism in sera	1400:1469	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	3	theme	inflammations	1495:1507	arg1	enhancement					1327:1337	enhancement	1327:1337	enhancement of cellular mitochondrial and ribosomal activities	1327:1388	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	3	theme	inflammations	1495:1507	arg1	Reduction					1286:1294	Reduction	1286:1294	Reduction of intestinal inflammation	1286:1321	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	3	theme	inflammations	1495:1507	arg1	inhibition					1476:1485	inhibition	1476:1485	inhibition of lung inflammations	1476:1507	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	3	theme	inflammations	1495:1507	arg1	mechanisms					1548:1557	the important COPD ameliorative mechanisms	1516:1557	the important COPD ameliorative mechanisms	1516:1557	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	2	4	theme	tract	320:324	arg1	microbiota					294:303	the microbiota	290:303	the microbiota of respiratory tract	290:324	Although the microbiota of respiratory tract is established to be associated with COPD, the causality of gut microbiota in COPD development is not yet established.
33687943	12	5	dep	CONCLUSION	1748:1757	arg1	connected					1797:1805	connected	1797:1805	was connected	1793:1805	CONCLUSION The gut microbiota-lung COPD axis was connected.
33687943	10	6	theme	ameliorative	1535:1546	arg1	restoration					1411:1421	systematic restoration	1400:1421	systematic restoration of aberrant host amino acids metabolism in sera	1400:1469	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	6	theme	ameliorative	1535:1546	arg1	mechanisms					1548:1557	the important COPD ameliorative mechanisms	1516:1557	the important COPD ameliorative mechanisms	1516:1557	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	6	theme	ameliorative	1535:1546	arg1	Reduction					1286:1294	Reduction	1286:1294	Reduction of intestinal inflammation	1286:1321	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	6	theme	ameliorative	1535:1546	arg1	inhibition					1476:1485	inhibition	1476:1485	inhibition of lung inflammations	1476:1507	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	6	theme	ameliorative	1535:1546	arg1	enhancement					1327:1337	enhancement	1327:1337	enhancement of cellular mitochondrial and ribosomal activities	1327:1388	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	2	7	from	causality	373:381	arg1	development					409:419	COPD development	404:419	COPD development	404:419	Although the microbiota of respiratory tract is established to be associated with COPD, the causality of gut microbiota in COPD development is not yet established.
33687943	13	8	theme	alternative	1906:1916	arg1	strain					1841:1846	A potentially benefial bacterial strain	1808:1846	A potentially benefial bacterial strain	1808:1846	A potentially benefial bacterial strain and its functional component may be developed and used as alternative agents for COPD prevention or treatment.
33687943	13	8	theme	alternative	1906:1916	arg1	agents					1918:1923	alternative agents	1906:1923	alternative agents	1906:1923	A potentially benefial bacterial strain and its functional component may be developed and used as alternative agents for COPD prevention or treatment.
33687943	10	9	theme	important	1520:1528	arg1	restoration					1411:1421	systematic restoration	1400:1421	systematic restoration of aberrant host amino acids metabolism in sera	1400:1469	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	9	theme	important	1520:1528	arg1	mechanisms					1548:1557	the important COPD ameliorative mechanisms	1516:1557	the important COPD ameliorative mechanisms	1516:1557	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	9	theme	important	1520:1528	arg1	Reduction					1286:1294	Reduction	1286:1294	Reduction of intestinal inflammation	1286:1321	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	9	theme	important	1520:1528	arg1	inhibition					1476:1485	inhibition	1476:1485	inhibition of lung inflammations	1476:1507	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	9	theme	important	1520:1528	arg1	enhancement					1327:1337	enhancement	1327:1337	enhancement of cellular mitochondrial and ribosomal activities	1327:1388	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	1	10	theme	chronic	210:216	arg1	obstruction					218:228	chronic obstruction	210:228	chronic obstruction of lung airflow	210:244	OBJECTIVE Chronic obstructive pulmonary disease (COPD) is a global disease characterised by chronic obstruction of lung airflow interfering with normal breathing.
33687943	8	11	theme	microbiota	1053:1062	arg1	composition					1064:1074	RESULTS Gut microbiota composition	1041:1074	RESULTS Gut microbiota composition	1041:1074	RESULTS Gut microbiota composition significantly affects CS-induced COPD development, and faecal microbiota transplantation restores COPD pathogenesis.
33687943	4	12	dep	DESIGN	627:632	arg1	model					672:676	A murine cigarette smoking (CS)-based model	634:676	DESIGN A murine cigarette smoking (CS)-based model of COPD and strategies evaluating causal effects of microbiota	627:739	DESIGN A murine cigarette smoking (CS)-based model of COPD and strategies evaluating causal effects of microbiota were performed.
33687943	3	13	theme	active	586:591	arg1	components					593:602	their derived active components	572:602	their derived active components for COPD amelioration	572:624	We aimed to address the connection between gut microbiota composition and lung COPD development, and characterise bacteria and their derived active components for COPD amelioration.
33687943	11	14	attach	derived	1592:1598	arg2	lipopolysaccharide					1573:1590	the lipopolysaccharide	1569:1590	the lipopolysaccharide derived from P. goldsteinii	1569:1618	Besides, the lipopolysaccharide derived from P. goldsteinii is anti-inflammatory, and significantly ameliorates COPD by acting as an antagonist of toll-like receptor 4 signalling pathway.
33687943	11	14	attach	derived	1592:1598	arg2	anti-inflammatory					1623:1639	anti-inflammatory	1623:1639	anti-inflammatory	1623:1639	Besides, the lipopolysaccharide derived from P. goldsteinii is anti-inflammatory, and significantly ameliorates COPD by acting as an antagonist of toll-like receptor 4 signalling pathway.
33687943	11	14	attach	derived	1592:1598	arg1	goldsteinii					1608:1618	P. goldsteinii	1605:1618	P. goldsteinii	1605:1618	Besides, the lipopolysaccharide derived from P. goldsteinii is anti-inflammatory, and significantly ameliorates COPD by acting as an antagonist of toll-like receptor 4 signalling pathway.
33687943	4	15	theme	-based	665:670	arg1	model					672:676	A murine cigarette smoking (CS)-based model	634:676	DESIGN A murine cigarette smoking (CS)-based model of COPD and strategies evaluating causal effects of microbiota	627:739	DESIGN A murine cigarette smoking (CS)-based model of COPD and strategies evaluating causal effects of microbiota were performed.
33687943	1	16	theme	airflow	238:244	arg1	obstruction					218:228	chronic obstruction	210:228	chronic obstruction of lung airflow	210:244	OBJECTIVE Chronic obstructive pulmonary disease (COPD) is a global disease characterised by chronic obstruction of lung airflow interfering with normal breathing.
33687943	10	17	from	restoration	1411:1421	arg1	colon					1393:1397	colon	1393:1397	colon	1393:1397	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	17	from	restoration	1411:1421	arg1	sera					1466:1469	sera	1466:1469	sera	1466:1469	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	4	18	theme	microbiota	730:739	arg1	effects					719:725	causal effects	712:725	causal effects of microbiota	712:739	DESIGN A murine cigarette smoking (CS)-based model of COPD and strategies evaluating causal effects of microbiota were performed.
33687943	10	19	theme	cellular	1342:1349	arg1	activities					1379:1388	cellular mitochondrial and ribosomal activities	1342:1388	cellular mitochondrial and ribosomal activities	1342:1388	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	3	20	link	derived	578:584	arg1	components					593:602	their derived active components	572:602	their derived active components for COPD amelioration	572:624	We aimed to address the connection between gut microbiota composition and lung COPD development, and characterise bacteria and their derived active components for COPD amelioration.
33687943	7	21	dep	isolated	1000:1007	arg1	followed					1010:1017	followed	1010:1017	followed by functional assays	1010:1038	Bacteria derived active component was isolated, followed by functional assays.
33687943	0	22	theme	anti-inflammatory	52:68	arg1	lipopolysaccharide					98:115	anti-inflammatory Parabacteroides goldsteinii lipopolysaccharide	52:115	anti-inflammatory Parabacteroides goldsteinii lipopolysaccharide	52:115	Gut microbiota modulates COPD pathogenesis: role of anti-inflammatory Parabacteroides goldsteinii lipopolysaccharide.
33687943	10	23	theme	activities	1379:1388	arg1	restoration					1411:1421	systematic restoration	1400:1421	systematic restoration of aberrant host amino acids metabolism in sera	1400:1469	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	23	theme	activities	1379:1388	arg1	enhancement					1327:1337	enhancement	1327:1337	enhancement of cellular mitochondrial and ribosomal activities	1327:1388	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	23	theme	activities	1379:1388	arg1	Reduction					1286:1294	Reduction	1286:1294	Reduction of intestinal inflammation	1286:1321	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	23	theme	activities	1379:1388	arg1	inhibition					1476:1485	inhibition	1476:1485	inhibition of lung inflammations	1476:1507	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	23	theme	activities	1379:1388	arg1	mechanisms					1548:1557	the important COPD ameliorative mechanisms	1516:1557	the important COPD ameliorative mechanisms	1516:1557	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	0	24	theme	goldsteinii	86:96	arg1	lipopolysaccharide					98:115	anti-inflammatory Parabacteroides goldsteinii lipopolysaccharide	52:115	anti-inflammatory Parabacteroides goldsteinii lipopolysaccharide	52:115	Gut microbiota modulates COPD pathogenesis: role of anti-inflammatory Parabacteroides goldsteinii lipopolysaccharide.
33687943	1	25	theme	global	178:183	arg1	disease					185:191	a global disease	176:191	a global disease characterised by chronic obstruction of lung airflow interfering with normal breathing	176:278	OBJECTIVE Chronic obstructive pulmonary disease (COPD) is a global disease characterised by chronic obstruction of lung airflow interfering with normal breathing.
33687943	1	25	theme	global	178:183	arg1	disease					158:164	OBJECTIVE Chronic obstructive pulmonary disease	118:164	OBJECTIVE Chronic obstructive pulmonary disease (COPD)	118:171	OBJECTIVE Chronic obstructive pulmonary disease (COPD) is a global disease characterised by chronic obstruction of lung airflow interfering with normal breathing.
33687943	5	26	theme	Gut	757:759	arg1	structure					772:780	Gut microbiota structure	757:780	Gut microbiota structure	757:780	Gut microbiota structure was analysed, followed by isolation of target bacterium.
33687943	8	27	theme	CS-induced	1098:1107	arg1	development					1114:1124	CS-induced COPD development	1098:1124	CS-induced COPD development	1098:1124	RESULTS Gut microbiota composition significantly affects CS-induced COPD development, and faecal microbiota transplantation restores COPD pathogenesis.
33687943	13	28	theme	benefial	1822:1829	arg1	strain					1841:1846	A potentially benefial bacterial strain	1808:1846	A potentially benefial bacterial strain	1808:1846	A potentially benefial bacterial strain and its functional component may be developed and used as alternative agents for COPD prevention or treatment.
33687943	13	28	theme	benefial	1822:1829	arg1	agents					1918:1923	alternative agents	1906:1923	alternative agents	1906:1923	A potentially benefial bacterial strain and its functional component may be developed and used as alternative agents for COPD prevention or treatment.
33687943	10	29	theme	systematic	1400:1409	arg1	Reduction					1286:1294	Reduction	1286:1294	Reduction of intestinal inflammation	1286:1321	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	29	theme	systematic	1400:1409	arg1	mechanisms					1548:1557	the important COPD ameliorative mechanisms	1516:1557	the important COPD ameliorative mechanisms	1516:1557	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	29	theme	systematic	1400:1409	arg1	restoration					1411:1421	systematic restoration	1400:1421	systematic restoration of aberrant host amino acids metabolism in sera	1400:1469	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	29	theme	systematic	1400:1409	arg1	inhibition					1476:1485	inhibition	1476:1485	inhibition of lung inflammations	1476:1507	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	29	theme	systematic	1400:1409	arg1	enhancement					1327:1337	enhancement	1327:1337	enhancement of cellular mitochondrial and ribosomal activities	1327:1388	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	7	30	theme	active	979:984	arg1	component					986:994	active component	979:994	active component	979:994	Bacteria derived active component was isolated, followed by functional assays.
33687943	2	31	theme	COPD	404:407	arg1	development					409:419	COPD development	404:419	COPD development	404:419	Although the microbiota of respiratory tract is established to be associated with COPD, the causality of gut microbiota in COPD development is not yet established.
33687943	0	32	theme	Gut	0:2	arg1	microbiota					4:13	Gut microbiota	0:13	Gut microbiota	0:13	Gut microbiota modulates COPD pathogenesis: role of anti-inflammatory Parabacteroides goldsteinii lipopolysaccharide.
33687943	1	33	theme	OBJECTIVE	118:126	arg1	disease					185:191	a global disease	176:191	a global disease characterised by chronic obstruction of lung airflow interfering with normal breathing	176:278	OBJECTIVE Chronic obstructive pulmonary disease (COPD) is a global disease characterised by chronic obstruction of lung airflow interfering with normal breathing.
33687943	1	33	theme	OBJECTIVE	118:126	arg1	COPD					167:170	COPD	167:170	COPD	167:170	OBJECTIVE Chronic obstructive pulmonary disease (COPD) is a global disease characterised by chronic obstruction of lung airflow interfering with normal breathing.
33687943	1	33	theme	OBJECTIVE	118:126	arg1	disease					158:164	OBJECTIVE Chronic obstructive pulmonary disease	118:164	OBJECTIVE Chronic obstructive pulmonary disease (COPD)	118:171	OBJECTIVE Chronic obstructive pulmonary disease (COPD) is a global disease characterised by chronic obstruction of lung airflow interfering with normal breathing.
33687943	12	34	theme	COPD	1783:1786	arg1	axis					1788:1791	The gut microbiota-lung COPD axis	1759:1791	The gut microbiota-lung COPD axis	1759:1791	CONCLUSION The gut microbiota-lung COPD axis was connected.
33687943	10	35	theme	host	1435:1438	arg1	acids					1446:1450	host amino acids	1435:1450	aberrant host amino acids metabolism in sera	1426:1469	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	8	36	theme	microbiota	1138:1147	arg1	transplantation					1149:1163	faecal microbiota transplantation	1131:1163	faecal microbiota transplantation	1131:1163	RESULTS Gut microbiota composition significantly affects CS-induced COPD development, and faecal microbiota transplantation restores COPD pathogenesis.
33687943	1	37	theme	obstructive	136:146	arg1	disease					185:191	a global disease	176:191	a global disease characterised by chronic obstruction of lung airflow interfering with normal breathing	176:278	OBJECTIVE Chronic obstructive pulmonary disease (COPD) is a global disease characterised by chronic obstruction of lung airflow interfering with normal breathing.
33687943	1	37	theme	obstructive	136:146	arg1	COPD					167:170	COPD	167:170	COPD	167:170	OBJECTIVE Chronic obstructive pulmonary disease (COPD) is a global disease characterised by chronic obstruction of lung airflow interfering with normal breathing.
33687943	1	37	theme	obstructive	136:146	arg1	disease					158:164	OBJECTIVE Chronic obstructive pulmonary disease	118:164	OBJECTIVE Chronic obstructive pulmonary disease (COPD)	118:171	OBJECTIVE Chronic obstructive pulmonary disease (COPD) is a global disease characterised by chronic obstruction of lung airflow interfering with normal breathing.
33687943	9	38	theme	commensal	1195:1203	arg1	goldsteinii					1231:1241	A commensal bacterium Parabacteroides goldsteinii	1193:1241	A commensal bacterium Parabacteroides goldsteinii	1193:1241	A commensal bacterium Parabacteroides goldsteinii was isolated and shown to ameliorate COPD.
33687943	6	39	theme	sera	881:884	arg1	analyses					899:906	sera metabolomics analyses	881:906	sera metabolomics analyses	881:906	Single cell RNA sequencing, together with sera metabolomics analyses were performed to identify host responsive molecules.
33687943	10	40	from	inhibition	1476:1485	arg1	colon					1393:1397	colon	1393:1397	colon	1393:1397	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	40	from	inhibition	1476:1485	arg1	sera					1466:1469	sera	1466:1469	sera	1466:1469	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	9	41	theme	Parabacteroides	1215:1229	arg1	goldsteinii					1231:1241	A commensal bacterium Parabacteroides goldsteinii	1193:1241	A commensal bacterium Parabacteroides goldsteinii	1193:1241	A commensal bacterium Parabacteroides goldsteinii was isolated and shown to ameliorate COPD.
33687943	5	42	theme	target	821:826	arg1	bacterium					828:836	target bacterium	821:836	target bacterium	821:836	Gut microbiota structure was analysed, followed by isolation of target bacterium.
33687943	11	43	theme	signalling	1728:1737	arg1	pathway					1739:1745	toll-like receptor 4 signalling pathway	1707:1745	toll-like receptor 4 signalling pathway	1707:1745	Besides, the lipopolysaccharide derived from P. goldsteinii is anti-inflammatory, and significantly ameliorates COPD by acting as an antagonist of toll-like receptor 4 signalling pathway.
33687943	10	44	theme	acids	1446:1450	arg1	metabolism					1452:1461	aberrant host amino acids metabolism	1426:1461	aberrant host amino acids metabolism in sera	1426:1469	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	3	45	theme	microbiota	492:501	arg1	composition					503:513	gut microbiota composition	488:513	gut microbiota composition	488:513	We aimed to address the connection between gut microbiota composition and lung COPD development, and characterise bacteria and their derived active components for COPD amelioration.
33687943	0	46	dep	modulates	15:23	arg1	role					44:47	role	44:47	role of anti-inflammatory Parabacteroides goldsteinii lipopolysaccharide	44:115	Gut microbiota modulates COPD pathogenesis: role of anti-inflammatory Parabacteroides goldsteinii lipopolysaccharide.
33687943	2	47	theme	microbiota	390:399	arg1	causality					373:381	the causality	369:381	the causality of gut microbiota in COPD development	369:419	Although the microbiota of respiratory tract is established to be associated with COPD, the causality of gut microbiota in COPD development is not yet established.
33687943	1	48	theme	normal	263:268	arg1	breathing					270:278	normal breathing	263:278	normal breathing	263:278	OBJECTIVE Chronic obstructive pulmonary disease (COPD) is a global disease characterised by chronic obstruction of lung airflow interfering with normal breathing.
33687943	4	49	theme	cigarette	643:651	arg1	smoking					653:659	cigarette smoking	643:659	cigarette smoking	643:659	DESIGN A murine cigarette smoking (CS)-based model of COPD and strategies evaluating causal effects of microbiota were performed.
33687943	3	50	theme	COPD	524:527	arg1	development					529:539	lung COPD development	519:539	lung COPD development	519:539	We aimed to address the connection between gut microbiota composition and lung COPD development, and characterise bacteria and their derived active components for COPD amelioration.
33687943	6	51	theme	responsive	940:949	arg1	molecules					951:959	host responsive molecules	935:959	host responsive molecules	935:959	Single cell RNA sequencing, together with sera metabolomics analyses were performed to identify host responsive molecules.
33687943	13	52	theme	COPD	1929:1932	arg1	prevention					1934:1943	COPD prevention	1929:1943	COPD prevention	1929:1943	A potentially benefial bacterial strain and its functional component may be developed and used as alternative agents for COPD prevention or treatment.
33687943	8	53	theme	RESULTS	1041:1047	arg1	composition					1064:1074	RESULTS Gut microbiota composition	1041:1074	RESULTS Gut microbiota composition	1041:1074	RESULTS Gut microbiota composition significantly affects CS-induced COPD development, and faecal microbiota transplantation restores COPD pathogenesis.
33687943	6	54	theme	Single	839:844	arg1	sequencing					855:864	Single cell RNA sequencing	839:864	Single cell RNA sequencing	839:864	Single cell RNA sequencing, together with sera metabolomics analyses were performed to identify host responsive molecules.
33687943	10	55	theme	intestinal	1299:1308	arg1	inflammation					1310:1321	intestinal inflammation	1299:1321	intestinal inflammation	1299:1321	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	11	56	theme	pathway	1739:1745	arg1	antagonist					1693:1702	an antagonist	1690:1702	an antagonist of toll-like receptor 4 signalling pathway	1690:1745	Besides, the lipopolysaccharide derived from P. goldsteinii is anti-inflammatory, and significantly ameliorates COPD by acting as an antagonist of toll-like receptor 4 signalling pathway.
33687943	10	57	from	enhancement	1327:1337	arg1	colon					1393:1397	colon	1393:1397	colon	1393:1397	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	57	from	enhancement	1327:1337	arg1	sera					1466:1469	sera	1466:1469	sera	1466:1469	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	58	theme	lung	1490:1493	arg1	inflammations					1495:1507	lung inflammations	1490:1507	lung inflammations	1490:1507	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	2	59	theme	respiratory	308:318	arg1	tract					320:324	respiratory tract	308:324	respiratory tract	308:324	Although the microbiota of respiratory tract is established to be associated with COPD, the causality of gut microbiota in COPD development is not yet established.
33687943	11	60	theme	toll-like	1707:1715	arg1	receptor					1717:1724	toll-like receptor	1707:1724	toll-like receptor 4 signalling pathway	1707:1745	Besides, the lipopolysaccharide derived from P. goldsteinii is anti-inflammatory, and significantly ameliorates COPD by acting as an antagonist of toll-like receptor 4 signalling pathway.
33687943	8	61	theme	Gut	1049:1051	arg1	composition					1064:1074	RESULTS Gut microbiota composition	1041:1074	RESULTS Gut microbiota composition	1041:1074	RESULTS Gut microbiota composition significantly affects CS-induced COPD development, and faecal microbiota transplantation restores COPD pathogenesis.
33687943	10	62	theme	COPD	1530:1533	arg1	restoration					1411:1421	systematic restoration	1400:1421	systematic restoration of aberrant host amino acids metabolism in sera	1400:1469	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	62	theme	COPD	1530:1533	arg1	mechanisms					1548:1557	the important COPD ameliorative mechanisms	1516:1557	the important COPD ameliorative mechanisms	1516:1557	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	62	theme	COPD	1530:1533	arg1	Reduction					1286:1294	Reduction	1286:1294	Reduction of intestinal inflammation	1286:1321	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	62	theme	COPD	1530:1533	arg1	inhibition					1476:1485	inhibition	1476:1485	inhibition of lung inflammations	1476:1507	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	62	theme	COPD	1530:1533	arg1	enhancement					1327:1337	enhancement	1327:1337	enhancement of cellular mitochondrial and ribosomal activities	1327:1388	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	4	63	theme	COPD	681:684	arg1	model					672:676	A murine cigarette smoking (CS)-based model	634:676	DESIGN A murine cigarette smoking (CS)-based model of COPD and strategies evaluating causal effects of microbiota	627:739	DESIGN A murine cigarette smoking (CS)-based model of COPD and strategies evaluating causal effects of microbiota were performed.
33687943	3	64	theme	derived	578:584	arg1	components					593:602	their derived active components	572:602	their derived active components for COPD amelioration	572:624	We aimed to address the connection between gut microbiota composition and lung COPD development, and characterise bacteria and their derived active components for COPD amelioration.
33687943	12	65	theme	gut	1763:1765	arg1	axis					1788:1791	The gut microbiota-lung COPD axis	1759:1791	The gut microbiota-lung COPD axis	1759:1791	CONCLUSION The gut microbiota-lung COPD axis was connected.
33687943	11	66	theme	receptor	1717:1724	arg1	pathway					1739:1745	toll-like receptor 4 signalling pathway	1707:1745	toll-like receptor 4 signalling pathway	1707:1745	Besides, the lipopolysaccharide derived from P. goldsteinii is anti-inflammatory, and significantly ameliorates COPD by acting as an antagonist of toll-like receptor 4 signalling pathway.
33687943	10	67	from	Reduction	1286:1294	arg1	colon					1393:1397	colon	1393:1397	colon	1393:1397	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	67	from	Reduction	1286:1294	arg1	sera					1466:1469	sera	1466:1469	sera	1466:1469	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	1	68	theme	lung	233:236	arg1	airflow					238:244	lung airflow	233:244	lung airflow	233:244	OBJECTIVE Chronic obstructive pulmonary disease (COPD) is a global disease characterised by chronic obstruction of lung airflow interfering with normal breathing.
33687943	5	69	dep	analysed	786:793	arg1	followed					796:803	followed	796:803	followed by isolation of target bacterium	796:836	Gut microbiota structure was analysed, followed by isolation of target bacterium.
33687943	3	70	theme	COPD	608:611	arg1	amelioration					613:624	COPD amelioration	608:624	COPD amelioration	608:624	We aimed to address the connection between gut microbiota composition and lung COPD development, and characterise bacteria and their derived active components for COPD amelioration.
33687943	1	71	theme	pulmonary	148:156	arg1	disease					185:191	a global disease	176:191	a global disease characterised by chronic obstruction of lung airflow interfering with normal breathing	176:278	OBJECTIVE Chronic obstructive pulmonary disease (COPD) is a global disease characterised by chronic obstruction of lung airflow interfering with normal breathing.
33687943	1	71	theme	pulmonary	148:156	arg1	COPD					167:170	COPD	167:170	COPD	167:170	OBJECTIVE Chronic obstructive pulmonary disease (COPD) is a global disease characterised by chronic obstruction of lung airflow interfering with normal breathing.
33687943	1	71	theme	pulmonary	148:156	arg1	disease					158:164	OBJECTIVE Chronic obstructive pulmonary disease	118:164	OBJECTIVE Chronic obstructive pulmonary disease (COPD)	118:171	OBJECTIVE Chronic obstructive pulmonary disease (COPD) is a global disease characterised by chronic obstruction of lung airflow interfering with normal breathing.
33687943	10	72	theme	mitochondrial	1351:1363	arg1	activities					1379:1388	cellular mitochondrial and ribosomal activities	1342:1388	cellular mitochondrial and ribosomal activities	1342:1388	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	0	73	theme	Parabacteroides	70:84	arg1	lipopolysaccharide					98:115	anti-inflammatory Parabacteroides goldsteinii lipopolysaccharide	52:115	anti-inflammatory Parabacteroides goldsteinii lipopolysaccharide	52:115	Gut microbiota modulates COPD pathogenesis: role of anti-inflammatory Parabacteroides goldsteinii lipopolysaccharide.
33687943	13	74	used	used	1898:1901	arg2	agents					1918:1923	alternative agents	1906:1923	alternative agents	1906:1923	A potentially benefial bacterial strain and its functional component may be developed and used as alternative agents for COPD prevention or treatment.
33687943	13	74	used	used	1898:1901	arg2	strain					1841:1846	A potentially benefial bacterial strain	1808:1846	A potentially benefial bacterial strain	1808:1846	A potentially benefial bacterial strain and its functional component may be developed and used as alternative agents for COPD prevention or treatment.
33687943	4	75	theme	strategies	690:699	arg1	model					672:676	A murine cigarette smoking (CS)-based model	634:676	DESIGN A murine cigarette smoking (CS)-based model of COPD and strategies evaluating causal effects of microbiota	627:739	DESIGN A murine cigarette smoking (CS)-based model of COPD and strategies evaluating causal effects of microbiota were performed.
33687943	0	76	theme	lipopolysaccharide	98:115	arg1	role					44:47	role	44:47	role of anti-inflammatory Parabacteroides goldsteinii lipopolysaccharide	44:115	Gut microbiota modulates COPD pathogenesis: role of anti-inflammatory Parabacteroides goldsteinii lipopolysaccharide.
33687943	7	77	theme	functional	1022:1031	arg1	assays					1033:1038	functional assays	1022:1038	functional assays	1022:1038	Bacteria derived active component was isolated, followed by functional assays.
33687943	13	78	theme	functional	1856:1865	arg1	component					1867:1875	its functional component	1852:1875	its functional component	1852:1875	A potentially benefial bacterial strain and its functional component may be developed and used as alternative agents for COPD prevention or treatment.
33687943	4	79	theme	causal	712:717	arg1	effects					719:725	causal effects	712:725	causal effects of microbiota	712:739	DESIGN A murine cigarette smoking (CS)-based model of COPD and strategies evaluating causal effects of microbiota were performed.
33687943	10	80	theme	ribosomal	1369:1377	arg1	activities					1379:1388	cellular mitochondrial and ribosomal activities	1342:1388	cellular mitochondrial and ribosomal activities	1342:1388	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	8	81	theme	COPD	1109:1112	arg1	development					1114:1124	CS-induced COPD development	1098:1124	CS-induced COPD development	1098:1124	RESULTS Gut microbiota composition significantly affects CS-induced COPD development, and faecal microbiota transplantation restores COPD pathogenesis.
33687943	5	82	theme	microbiota	761:770	arg1	structure					772:780	Gut microbiota structure	757:780	Gut microbiota structure	757:780	Gut microbiota structure was analysed, followed by isolation of target bacterium.
33687943	10	83	from	metabolism	1452:1461	arg1	sera					1466:1469	sera	1466:1469	sera	1466:1469	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	13	84	theme	bacterial	1831:1839	arg1	strain					1841:1846	A potentially benefial bacterial strain	1808:1846	A potentially benefial bacterial strain	1808:1846	A potentially benefial bacterial strain and its functional component may be developed and used as alternative agents for COPD prevention or treatment.
33687943	13	84	theme	bacterial	1831:1839	arg1	agents					1918:1923	alternative agents	1906:1923	alternative agents	1906:1923	A potentially benefial bacterial strain and its functional component may be developed and used as alternative agents for COPD prevention or treatment.
33687943	5	85	theme	bacterium	828:836	arg1	isolation					808:816	isolation	808:816	isolation of target bacterium	808:836	Gut microbiota structure was analysed, followed by isolation of target bacterium.
33687943	8	86	theme	faecal	1131:1136	arg1	transplantation					1149:1163	faecal microbiota transplantation	1131:1163	faecal microbiota transplantation	1131:1163	RESULTS Gut microbiota composition significantly affects CS-induced COPD development, and faecal microbiota transplantation restores COPD pathogenesis.
33687943	10	87	from	sera	1466:1469	arg1	restoration					1411:1421	systematic restoration	1400:1421	systematic restoration of aberrant host amino acids metabolism in sera	1400:1469	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	87	from	sera	1466:1469	arg1	enhancement					1327:1337	enhancement	1327:1337	enhancement of cellular mitochondrial and ribosomal activities	1327:1388	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	87	from	sera	1466:1469	arg1	Reduction					1286:1294	Reduction	1286:1294	Reduction of intestinal inflammation	1286:1321	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	87	from	sera	1466:1469	arg1	inhibition					1476:1485	inhibition	1476:1485	inhibition of lung inflammations	1476:1507	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	87	from	sera	1466:1469	arg1	mechanisms					1548:1557	the important COPD ameliorative mechanisms	1516:1557	the important COPD ameliorative mechanisms	1516:1557	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	88	theme	amino	1440:1444	arg1	acids					1446:1450	host amino acids	1435:1450	aberrant host amino acids metabolism in sera	1426:1469	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	0	89	theme	COPD	25:28	arg1	pathogenesis					30:41	COPD pathogenesis	25:41	COPD pathogenesis	25:41	Gut microbiota modulates COPD pathogenesis: role of anti-inflammatory Parabacteroides goldsteinii lipopolysaccharide.
33687943	1	90	theme	Chronic	128:134	arg1	disease					185:191	a global disease	176:191	a global disease characterised by chronic obstruction of lung airflow interfering with normal breathing	176:278	OBJECTIVE Chronic obstructive pulmonary disease (COPD) is a global disease characterised by chronic obstruction of lung airflow interfering with normal breathing.
33687943	1	90	theme	Chronic	128:134	arg1	COPD					167:170	COPD	167:170	COPD	167:170	OBJECTIVE Chronic obstructive pulmonary disease (COPD) is a global disease characterised by chronic obstruction of lung airflow interfering with normal breathing.
33687943	1	90	theme	Chronic	128:134	arg1	disease					158:164	OBJECTIVE Chronic obstructive pulmonary disease	118:164	OBJECTIVE Chronic obstructive pulmonary disease (COPD)	118:171	OBJECTIVE Chronic obstructive pulmonary disease (COPD) is a global disease characterised by chronic obstruction of lung airflow interfering with normal breathing.
33687943	12	91	theme	microbiota-lung	1767:1781	arg1	axis					1788:1791	The gut microbiota-lung COPD axis	1759:1791	The gut microbiota-lung COPD axis	1759:1791	CONCLUSION The gut microbiota-lung COPD axis was connected.
33687943	6	92	dep	together	867:874	arg1	with					876:879	with	876:879	with	876:879	Single cell RNA sequencing, together with sera metabolomics analyses were performed to identify host responsive molecules.
33687943	10	93	theme	aberrant	1426:1433	arg1	metabolism					1452:1461	aberrant host amino acids metabolism	1426:1461	aberrant host amino acids metabolism in sera	1426:1469	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	3	94	theme	gut	488:490	arg1	composition					503:513	gut microbiota composition	488:513	gut microbiota composition	488:513	We aimed to address the connection between gut microbiota composition and lung COPD development, and characterise bacteria and their derived active components for COPD amelioration.
33687943	8	95	theme	COPD	1174:1177	arg1	pathogenesis					1179:1190	COPD pathogenesis	1174:1190	COPD pathogenesis	1174:1190	RESULTS Gut microbiota composition significantly affects CS-induced COPD development, and faecal microbiota transplantation restores COPD pathogenesis.
33687943	6	96	theme	metabolomics	886:897	arg1	analyses					899:906	sera metabolomics analyses	881:906	sera metabolomics analyses	881:906	Single cell RNA sequencing, together with sera metabolomics analyses were performed to identify host responsive molecules.
33687943	4	97	dep	-based	665:670	arg1	smoking					653:659	cigarette smoking	643:659	cigarette smoking	643:659	DESIGN A murine cigarette smoking (CS)-based model of COPD and strategies evaluating causal effects of microbiota were performed.
33687943	9	98	theme	bacterium	1205:1213	arg1	goldsteinii					1231:1241	A commensal bacterium Parabacteroides goldsteinii	1193:1241	A commensal bacterium Parabacteroides goldsteinii	1193:1241	A commensal bacterium Parabacteroides goldsteinii was isolated and shown to ameliorate COPD.
33687943	10	99	theme	metabolism	1452:1461	arg1	restoration					1411:1421	systematic restoration	1400:1421	systematic restoration of aberrant host amino acids metabolism in sera	1400:1469	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	99	theme	metabolism	1452:1461	arg1	enhancement					1327:1337	enhancement	1327:1337	enhancement of cellular mitochondrial and ribosomal activities	1327:1388	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	99	theme	metabolism	1452:1461	arg1	Reduction					1286:1294	Reduction	1286:1294	Reduction of intestinal inflammation	1286:1321	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	99	theme	metabolism	1452:1461	arg1	inhibition					1476:1485	inhibition	1476:1485	inhibition of lung inflammations	1476:1507	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	10	99	theme	metabolism	1452:1461	arg1	mechanisms					1548:1557	the important COPD ameliorative mechanisms	1516:1557	the important COPD ameliorative mechanisms	1516:1557	Reduction of intestinal inflammation and enhancement of cellular mitochondrial and ribosomal activities in colon, systematic restoration of aberrant host amino acids metabolism in sera, and inhibition of lung inflammations act as the important COPD ameliorative mechanisms.
33687943	3	100	theme	lung	519:522	arg1	development					529:539	lung COPD development	519:539	lung COPD development	519:539	We aimed to address the connection between gut microbiota composition and lung COPD development, and characterise bacteria and their derived active components for COPD amelioration.
33687943	4	101	theme	murine	636:641	arg1	model					672:676	A murine cigarette smoking (CS)-based model	634:676	DESIGN A murine cigarette smoking (CS)-based model of COPD and strategies evaluating causal effects of microbiota	627:739	DESIGN A murine cigarette smoking (CS)-based model of COPD and strategies evaluating causal effects of microbiota were performed.
33687943	6	102	theme	RNA	851:853	arg1	sequencing					855:864	Single cell RNA sequencing	839:864	Single cell RNA sequencing	839:864	Single cell RNA sequencing, together with sera metabolomics analyses were performed to identify host responsive molecules.
33687943	2	103	theme	gut	386:388	arg1	microbiota					390:399	gut microbiota	386:399	gut microbiota	386:399	Although the microbiota of respiratory tract is established to be associated with COPD, the causality of gut microbiota in COPD development is not yet established.
31968646	8	0	theme	glucose	1546:1552	arg1	disorders					1575:1583	glucose and lipid metabolism disorders	1546:1583	glucose and lipid metabolism disorders	1546:1583	To the best of our knowledge, this study is the first to illustrate that COS mediate glucose and lipid metabolism disorders by regulating gut microbiota and SMYD3-mediated signaling pathways.
31968646	1	1	theme	activities	174:183	arg1	variety					152:158	a variety	150:158	a variety of biological activities	150:183	Chitooligosaccharides (COS) have a variety of biological activities due to their positively charged amino groups.
31968646	1	1	theme	activities	174:183	arg1	activities					174:183	biological activities	163:183	biological activities	163:183	Chitooligosaccharides (COS) have a variety of biological activities due to their positively charged amino groups.
31968646	2	2	contain	have	259:262	arg1	COS					255:257	COS	255:257	COS	255:257	Studies have shown that COS have antidiabetic effects, but their molecular mechanism has not been fully elucidated.
31968646	2	2	contain	have	259:262	arg2	effects					277:283	antidiabetic effects	264:283	antidiabetic effects	264:283	Studies have shown that COS have antidiabetic effects, but their molecular mechanism has not been fully elucidated.
31968646	0	3	theme	Gut	101:103	arg1	Microflora					105:114	Gut Microflora	101:114	Gut Microflora	101:114	Chitooligosaccharides Modulate Glucose-Lipid Metabolism by Suppressing SMYD3 Pathways and Regulating Gut Microflora.
31968646	1	4	contain	have	145:148	arg1	Chitooligosaccharides					117:137	Chitooligosaccharides	117:137	Chitooligosaccharides (COS)	117:143	Chitooligosaccharides (COS) have a variety of biological activities due to their positively charged amino groups.
31968646	1	4	contain	have	145:148	arg2	activities					174:183	biological activities	163:183	biological activities	163:183	Chitooligosaccharides (COS) have a variety of biological activities due to their positively charged amino groups.
31968646	1	4	contain	have	145:148	arg2	variety					152:158	a variety	150:158	a variety of biological activities	150:183	Chitooligosaccharides (COS) have a variety of biological activities due to their positively charged amino groups.
31968646	1	4	contain	have	145:148	arg1	COS					140:142	COS	140:142	COS	140:142	Chitooligosaccharides (COS) have a variety of biological activities due to their positively charged amino groups.
31968646	4	5	theme	Bacteroidetes	677:689	arg1	abundance					652:660	the abundance	648:660	the abundance of Firmicutes, Bacteroidetes, and Proteobacteria	648:709	Additionally, treatment with COS can modulate the composition of the gut microbiota in the colon by altering the abundance of Firmicutes, Bacteroidetes, and Proteobacteria.
31968646	4	6	from	composition	589:599	arg1	colon					630:634	the colon	626:634	the colon	626:634	Additionally, treatment with COS can modulate the composition of the gut microbiota in the colon by altering the abundance of Firmicutes, Bacteroidetes, and Proteobacteria.
31968646	5	7	dep	enzymes	799:805	arg1	GLP-1					886:890	incretin glucagon-like peptide 1 (GLP-1)	852:891	incretin glucagon-like peptide 1 (GLP-1)	852:891	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	5	7	dep	enzymes	799:805	arg1	7-alpha-hydroxylase					819:837	cholesterol 7-alpha-hydroxylase	807:837	cholesterol 7-alpha-hydroxylase (CYP7A1)	807:846	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	5	7	dep	enzymes	799:805	arg1	CYP7A1					840:845	CYP7A1	840:845	CYP7A1	840:845	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	5	7	dep	enzymes	799:805	arg1	enzymes					799:805	the cholesterol-degrading enzymes	773:805	the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1)	773:891	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	5	8	theme	A	995:995	arg1	reductase					997:1005	3-hydroxy-3-methylglutaryl coenzyme A reductase	959:1005	3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR)	959:1013	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	5	8	theme	A	995:995	arg1	enzyme					1024:1029	the key enzyme	1016:1029	the key enzyme in cholesterol synthesis	1016:1054	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	5	8	theme	A	995:995	arg1	HMGCR					1008:1012	HMGCR	1008:1012	HMGCR	1008:1012	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	5	9	theme	coenzyme	986:993	arg1	reductase					997:1005	3-hydroxy-3-methylglutaryl coenzyme A reductase	959:1005	3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR)	959:1013	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	5	9	theme	coenzyme	986:993	arg1	enzyme					1024:1029	the key enzyme	1016:1029	the key enzyme in cholesterol synthesis	1016:1054	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	5	9	theme	coenzyme	986:993	arg1	HMGCR					1008:1012	HMGCR	1008:1012	HMGCR	1008:1012	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	7	10	theme	hypolipidemic	1436:1448	arg1	functions					1450:1458	hypolipidemic functions	1436:1458	hypolipidemic functions	1436:1458	Overexpression of SMYD3 can suppress the inhibitory effect of COS on HMGCR, and COS might regulate HMGCR by inhibiting SMYD3, thereby exerting hypolipidemic functions.
31968646	5	11	theme	reductase	997:1005	arg1	expression					945:954	expression	945:954	expression	945:954	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	5	11	theme	reductase	997:1005	arg1	transcription					927:939	transcription	927:939	transcription	927:939	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	2	12	theme	molecular	296:304	arg1	mechanism					306:314	their molecular mechanism	290:314	their molecular mechanism	290:314	Studies have shown that COS have antidiabetic effects, but their molecular mechanism has not been fully elucidated.
31968646	5	13	theme	T2DM	728:731	arg1	mice					733:736	T2DM mice	728:736	T2DM mice	728:736	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	4	14	with	treatment	553:561	arg1	COS					568:570	COS	568:570	COS	568:570	Additionally, treatment with COS can modulate the composition of the gut microbiota in the colon by altering the abundance of Firmicutes, Bacteroidetes, and Proteobacteria.
31968646	7	15	theme	SMYD3	1311:1315	arg1	Overexpression					1293:1306	Overexpression	1293:1306	Overexpression of SMYD3	1293:1315	Overexpression of SMYD3 can suppress the inhibitory effect of COS on HMGCR, and COS might regulate HMGCR by inhibiting SMYD3, thereby exerting hypolipidemic functions.
31968646	5	16	theme	glucagon-like	861:873	arg1	GLP-1					886:890	incretin glucagon-like peptide 1 (GLP-1)	852:891	incretin glucagon-like peptide 1 (GLP-1)	852:891	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	5	16	theme	glucagon-like	861:873	arg1	enzymes					799:805	the cholesterol-degrading enzymes	773:805	the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1)	773:891	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	5	17	theme	cholesterol-degrading	777:797	arg1	GLP-1					886:890	incretin glucagon-like peptide 1 (GLP-1)	852:891	incretin glucagon-like peptide 1 (GLP-1)	852:891	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	5	17	theme	cholesterol-degrading	777:797	arg1	7-alpha-hydroxylase					819:837	cholesterol 7-alpha-hydroxylase	807:837	cholesterol 7-alpha-hydroxylase (CYP7A1)	807:846	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	5	17	theme	cholesterol-degrading	777:797	arg1	enzymes					799:805	the cholesterol-degrading enzymes	773:805	the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1)	773:891	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	3	18	with	mice	496:499	arg1	T2DM					532:535	T2DM	532:535	T2DM	532:535	The present study confirmed that COS can reduce hyperglycemia and hyperlipidemia, prevent obesity, and enhance histological changes in the livers of mice with type 2 diabetes mellitus (T2DM).
31968646	3	18	with	mice	496:499	arg1	mellitus					522:529	type 2 diabetes mellitus	506:529	type 2 diabetes mellitus (T2DM)	506:536	The present study confirmed that COS can reduce hyperglycemia and hyperlipidemia, prevent obesity, and enhance histological changes in the livers of mice with type 2 diabetes mellitus (T2DM).
31968646	8	19	theme	lipid	1558:1562	arg1	metabolism					1564:1573	lipid metabolism	1558:1573	lipid metabolism	1558:1573	To the best of our knowledge, this study is the first to illustrate that COS mediate glucose and lipid metabolism disorders by regulating gut microbiota and SMYD3-mediated signaling pathways.
31968646	6	20	theme	steatosis	1109:1117	arg1	model					1119:1123	an oleic acid-induced hepatocyte steatosis model	1076:1123	an oleic acid-induced hepatocyte steatosis model	1076:1123	Furthermore, using an oleic acid-induced hepatocyte steatosis model, we found that HMGCR can be directly transactivated by SET and MYND domain containing 3 (SMYD3), a transcriptional regulator, via 5'-CCCTCC-3' element in the promoter.
31968646	3	21	theme	histological	458:469	arg1	changes					471:477	histological changes	458:477	histological changes in the livers of mice with type 2 diabetes mellitus (T2DM)	458:536	The present study confirmed that COS can reduce hyperglycemia and hyperlipidemia, prevent obesity, and enhance histological changes in the livers of mice with type 2 diabetes mellitus (T2DM).
31968646	6	22	theme	oleic	1079:1083	arg1	model					1119:1123	an oleic acid-induced hepatocyte steatosis model	1076:1123	an oleic acid-induced hepatocyte steatosis model	1076:1123	Furthermore, using an oleic acid-induced hepatocyte steatosis model, we found that HMGCR can be directly transactivated by SET and MYND domain containing 3 (SMYD3), a transcriptional regulator, via 5'-CCCTCC-3' element in the promoter.
31968646	5	23	theme	cholesterol	807:817	arg1	CYP7A1					840:845	CYP7A1	840:845	CYP7A1	840:845	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	5	23	theme	cholesterol	807:817	arg1	7-alpha-hydroxylase					819:837	cholesterol 7-alpha-hydroxylase	807:837	cholesterol 7-alpha-hydroxylase (CYP7A1)	807:846	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	5	23	theme	cholesterol	807:817	arg1	enzymes					799:805	the cholesterol-degrading enzymes	773:805	the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1)	773:891	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	1	24	theme	charged	209:215	arg1	groups					223:228	their positively charged amino groups	192:228	their positively charged amino groups	192:228	Chitooligosaccharides (COS) have a variety of biological activities due to their positively charged amino groups.
31968646	6	25	theme	acid-induced	1085:1096	arg1	model					1119:1123	an oleic acid-induced hepatocyte steatosis model	1076:1123	an oleic acid-induced hepatocyte steatosis model	1076:1123	Furthermore, using an oleic acid-induced hepatocyte steatosis model, we found that HMGCR can be directly transactivated by SET and MYND domain containing 3 (SMYD3), a transcriptional regulator, via 5'-CCCTCC-3' element in the promoter.
31968646	5	26	theme	3-hydroxy-3-methylglutaryl	959:984	arg1	reductase					997:1005	3-hydroxy-3-methylglutaryl coenzyme A reductase	959:1005	3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR)	959:1013	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	5	26	theme	3-hydroxy-3-methylglutaryl	959:984	arg1	enzyme					1024:1029	the key enzyme	1016:1029	the key enzyme in cholesterol synthesis	1016:1054	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	5	26	theme	3-hydroxy-3-methylglutaryl	959:984	arg1	HMGCR					1008:1012	HMGCR	1008:1012	HMGCR	1008:1012	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	1	27	theme	amino	217:221	arg1	groups					223:228	their positively charged amino groups	192:228	their positively charged amino groups	192:228	Chitooligosaccharides (COS) have a variety of biological activities due to their positively charged amino groups.
31968646	0	28	theme	Glucose-Lipid	31:43	arg1	Metabolism					45:54	Glucose-Lipid Metabolism	31:54	Glucose-Lipid Metabolism	31:54	Chitooligosaccharides Modulate Glucose-Lipid Metabolism by Suppressing SMYD3 Pathways and Regulating Gut Microflora.
31968646	3	29	theme	diabetes	513:520	arg1	T2DM					532:535	T2DM	532:535	T2DM	532:535	The present study confirmed that COS can reduce hyperglycemia and hyperlipidemia, prevent obesity, and enhance histological changes in the livers of mice with type 2 diabetes mellitus (T2DM).
31968646	3	29	theme	diabetes	513:520	arg1	mellitus					522:529	type 2 diabetes mellitus	506:529	type 2 diabetes mellitus (T2DM)	506:536	The present study confirmed that COS can reduce hyperglycemia and hyperlipidemia, prevent obesity, and enhance histological changes in the livers of mice with type 2 diabetes mellitus (T2DM).
31968646	5	30	from	enzyme	1024:1029	arg1	synthesis					1046:1054	cholesterol synthesis	1034:1054	cholesterol synthesis	1034:1054	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	7	31	from	effect	1345:1350	arg1	HMGCR					1362:1366	HMGCR	1362:1366	HMGCR	1362:1366	Overexpression of SMYD3 can suppress the inhibitory effect of COS on HMGCR, and COS might regulate HMGCR by inhibiting SMYD3, thereby exerting hypolipidemic functions.
31968646	6	32	theme	transcriptional	1224:1238	arg1	regulator					1240:1248	a transcriptional regulator	1222:1248	a transcriptional regulator	1222:1248	Furthermore, using an oleic acid-induced hepatocyte steatosis model, we found that HMGCR can be directly transactivated by SET and MYND domain containing 3 (SMYD3), a transcriptional regulator, via 5'-CCCTCC-3' element in the promoter.
31968646	6	32	theme	transcriptional	1224:1238	arg1	domain					1193:1198	MYND domain containing 3	1188:1211	MYND domain containing 3 (SMYD3)	1188:1219	Furthermore, using an oleic acid-induced hepatocyte steatosis model, we found that HMGCR can be directly transactivated by SET and MYND domain containing 3 (SMYD3), a transcriptional regulator, via 5'-CCCTCC-3' element in the promoter.
31968646	6	33	theme	containing	1200:1209	arg1	regulator					1240:1248	a transcriptional regulator	1222:1248	a transcriptional regulator	1222:1248	Furthermore, using an oleic acid-induced hepatocyte steatosis model, we found that HMGCR can be directly transactivated by SET and MYND domain containing 3 (SMYD3), a transcriptional regulator, via 5'-CCCTCC-3' element in the promoter.
31968646	6	33	theme	containing	1200:1209	arg1	SMYD3					1214:1218	SMYD3	1214:1218	SMYD3	1214:1218	Furthermore, using an oleic acid-induced hepatocyte steatosis model, we found that HMGCR can be directly transactivated by SET and MYND domain containing 3 (SMYD3), a transcriptional regulator, via 5'-CCCTCC-3' element in the promoter.
31968646	6	33	theme	containing	1200:1209	arg1	domain					1193:1198	MYND domain containing 3	1188:1211	MYND domain containing 3 (SMYD3)	1188:1219	Furthermore, using an oleic acid-induced hepatocyte steatosis model, we found that HMGCR can be directly transactivated by SET and MYND domain containing 3 (SMYD3), a transcriptional regulator, via 5'-CCCTCC-3' element in the promoter.
31968646	6	34	theme	hepatocyte	1098:1107	arg1	model					1119:1123	an oleic acid-induced hepatocyte steatosis model	1076:1123	an oleic acid-induced hepatocyte steatosis model	1076:1123	Furthermore, using an oleic acid-induced hepatocyte steatosis model, we found that HMGCR can be directly transactivated by SET and MYND domain containing 3 (SMYD3), a transcriptional regulator, via 5'-CCCTCC-3' element in the promoter.
31968646	3	35	from	changes	471:477	arg1	livers					486:491	the livers	482:491	the livers of mice with type 2 diabetes mellitus (T2DM)	482:536	The present study confirmed that COS can reduce hyperglycemia and hyperlipidemia, prevent obesity, and enhance histological changes in the livers of mice with type 2 diabetes mellitus (T2DM).
31968646	5	36	theme	incretin	852:859	arg1	GLP-1					886:890	incretin glucagon-like peptide 1 (GLP-1)	852:891	incretin glucagon-like peptide 1 (GLP-1)	852:891	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	5	36	theme	incretin	852:859	arg1	enzymes					799:805	the cholesterol-degrading enzymes	773:805	the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1)	773:891	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	7	37	theme	inhibitory	1334:1343	arg1	effect					1345:1350	the inhibitory effect	1330:1350	the inhibitory effect of COS on HMGCR	1330:1366	Overexpression of SMYD3 can suppress the inhibitory effect of COS on HMGCR, and COS might regulate HMGCR by inhibiting SMYD3, thereby exerting hypolipidemic functions.
31968646	5	38	theme	cholesterol	1034:1044	arg1	synthesis					1046:1054	cholesterol synthesis	1034:1054	cholesterol synthesis	1034:1054	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	4	39	theme	Proteobacteria	696:709	arg1	abundance					652:660	the abundance	648:660	the abundance of Firmicutes, Bacteroidetes, and Proteobacteria	648:709	Additionally, treatment with COS can modulate the composition of the gut microbiota in the colon by altering the abundance of Firmicutes, Bacteroidetes, and Proteobacteria.
31968646	0	40	theme	SMYD3	71:75	arg1	Pathways					77:84	SMYD3 Pathways	71:84	SMYD3 Pathways	71:84	Chitooligosaccharides Modulate Glucose-Lipid Metabolism by Suppressing SMYD3 Pathways and Regulating Gut Microflora.
31968646	6	41	theme	MYND	1188:1191	arg1	regulator					1240:1248	a transcriptional regulator	1222:1248	a transcriptional regulator	1222:1248	Furthermore, using an oleic acid-induced hepatocyte steatosis model, we found that HMGCR can be directly transactivated by SET and MYND domain containing 3 (SMYD3), a transcriptional regulator, via 5'-CCCTCC-3' element in the promoter.
31968646	6	41	theme	MYND	1188:1191	arg1	SMYD3					1214:1218	SMYD3	1214:1218	SMYD3	1214:1218	Furthermore, using an oleic acid-induced hepatocyte steatosis model, we found that HMGCR can be directly transactivated by SET and MYND domain containing 3 (SMYD3), a transcriptional regulator, via 5'-CCCTCC-3' element in the promoter.
31968646	6	41	theme	MYND	1188:1191	arg1	domain					1193:1198	MYND domain containing 3	1188:1211	MYND domain containing 3 (SMYD3)	1188:1219	Furthermore, using an oleic acid-induced hepatocyte steatosis model, we found that HMGCR can be directly transactivated by SET and MYND domain containing 3 (SMYD3), a transcriptional regulator, via 5'-CCCTCC-3' element in the promoter.
31968646	1	42	theme	biological	163:172	arg1	activities					174:183	biological activities	163:183	biological activities	163:183	Chitooligosaccharides (COS) have a variety of biological activities due to their positively charged amino groups.
31968646	3	43	theme	type	506:509	arg1	T2DM					532:535	T2DM	532:535	T2DM	532:535	The present study confirmed that COS can reduce hyperglycemia and hyperlipidemia, prevent obesity, and enhance histological changes in the livers of mice with type 2 diabetes mellitus (T2DM).
31968646	3	43	theme	type	506:509	arg1	mellitus					522:529	type 2 diabetes mellitus	506:529	type 2 diabetes mellitus (T2DM)	506:536	The present study confirmed that COS can reduce hyperglycemia and hyperlipidemia, prevent obesity, and enhance histological changes in the livers of mice with type 2 diabetes mellitus (T2DM).
31968646	8	44	theme	signaling	1633:1641	arg1	pathways					1643:1650	SMYD3-mediated signaling pathways	1618:1650	SMYD3-mediated signaling pathways	1618:1650	To the best of our knowledge, this study is the first to illustrate that COS mediate glucose and lipid metabolism disorders by regulating gut microbiota and SMYD3-mediated signaling pathways.
31968646	5	45	with	treatment	739:747	arg1	COS					754:756	COS	754:756	COS	754:756	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	8	46	theme	SMYD3-mediated	1618:1631	arg1	pathways					1643:1650	SMYD3-mediated signaling pathways	1618:1650	SMYD3-mediated signaling pathways	1618:1650	To the best of our knowledge, this study is the first to illustrate that COS mediate glucose and lipid metabolism disorders by regulating gut microbiota and SMYD3-mediated signaling pathways.
31968646	6	47	from	element	1268:1274	arg1	promoter					1283:1290	the promoter	1279:1290	the promoter	1279:1290	Furthermore, using an oleic acid-induced hepatocyte steatosis model, we found that HMGCR can be directly transactivated by SET and MYND domain containing 3 (SMYD3), a transcriptional regulator, via 5'-CCCTCC-3' element in the promoter.
31968646	7	48	theme	COS	1355:1357	arg1	effect					1345:1350	the inhibitory effect	1330:1350	the inhibitory effect of COS on HMGCR	1330:1366	Overexpression of SMYD3 can suppress the inhibitory effect of COS on HMGCR, and COS might regulate HMGCR by inhibiting SMYD3, thereby exerting hypolipidemic functions.
31968646	3	49	theme	mice	496:499	arg1	livers					486:491	the livers	482:491	the livers of mice with type 2 diabetes mellitus (T2DM)	482:536	The present study confirmed that COS can reduce hyperglycemia and hyperlipidemia, prevent obesity, and enhance histological changes in the livers of mice with type 2 diabetes mellitus (T2DM).
31968646	8	50	theme	metabolism	1564:1573	arg1	disorders					1575:1583	glucose and lipid metabolism disorders	1546:1583	glucose and lipid metabolism disorders	1546:1583	To the best of our knowledge, this study is the first to illustrate that COS mediate glucose and lipid metabolism disorders by regulating gut microbiota and SMYD3-mediated signaling pathways.
31968646	2	51	theme	antidiabetic	264:275	arg1	effects					277:283	antidiabetic effects	264:283	antidiabetic effects	264:283	Studies have shown that COS have antidiabetic effects, but their molecular mechanism has not been fully elucidated.
31968646	5	52	theme	peptide	875:881	arg1	GLP-1					886:890	incretin glucagon-like peptide 1 (GLP-1)	852:891	incretin glucagon-like peptide 1 (GLP-1)	852:891	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	5	52	theme	peptide	875:881	arg1	enzymes					799:805	the cholesterol-degrading enzymes	773:805	the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1)	773:891	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	4	53	theme	microbiota	612:621	arg1	composition					589:599	the composition	585:599	the composition of the gut microbiota in the colon	585:634	Additionally, treatment with COS can modulate the composition of the gut microbiota in the colon by altering the abundance of Firmicutes, Bacteroidetes, and Proteobacteria.
31968646	3	54	theme	present	351:357	arg1	study					359:363	The present study	347:363	The present study	347:363	The present study confirmed that COS can reduce hyperglycemia and hyperlipidemia, prevent obesity, and enhance histological changes in the livers of mice with type 2 diabetes mellitus (T2DM).
31968646	5	55	theme	key	1020:1022	arg1	reductase					997:1005	3-hydroxy-3-methylglutaryl coenzyme A reductase	959:1005	3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR)	959:1013	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	5	55	theme	key	1020:1022	arg1	enzyme					1024:1029	the key enzyme	1016:1029	the key enzyme in cholesterol synthesis	1016:1054	Furthermore, in T2DM mice, treatment with COS can upregulate the cholesterol-degrading enzymes cholesterol 7-alpha-hydroxylase (CYP7A1) and incretin glucagon-like peptide 1 (GLP-1) while specifically inhibiting the transcription and expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), the key enzyme in cholesterol synthesis.
31968646	4	56	theme	Firmicutes	665:674	arg1	abundance					652:660	the abundance	648:660	the abundance of Firmicutes, Bacteroidetes, and Proteobacteria	648:709	Additionally, treatment with COS can modulate the composition of the gut microbiota in the colon by altering the abundance of Firmicutes, Bacteroidetes, and Proteobacteria.
31968646	8	57	theme	gut	1599:1601	arg1	microbiota					1603:1612	gut microbiota	1599:1612	gut microbiota	1599:1612	To the best of our knowledge, this study is the first to illustrate that COS mediate glucose and lipid metabolism disorders by regulating gut microbiota and SMYD3-mediated signaling pathways.
31968646	4	58	theme	gut	608:610	arg1	microbiota					612:621	the gut microbiota	604:621	the gut microbiota	604:621	Additionally, treatment with COS can modulate the composition of the gut microbiota in the colon by altering the abundance of Firmicutes, Bacteroidetes, and Proteobacteria.
34057430	3	0	theme	heparin-modified	448:463	arg1	phosphate					473:481	heparin-modified calcium phosphate	448:481	heparin-modified calcium phosphate (CaP) nanoparticles loaded with a large number of IO nanocrystals via coprecipitation from a supersaturated CaP solution supplemented with heparin and ferucarbotran (IO nanocrystals coated with carboxydextran)	448:691	We recently fabricated heparin-modified calcium phosphate (CaP) nanoparticles loaded with a large number of IO nanocrystals via coprecipitation from a supersaturated CaP solution supplemented with heparin and ferucarbotran (IO nanocrystals coated with carboxydextran).
34057430	3	0	theme	heparin-modified	448:463	arg1	CaP					484:486	CaP	484:486	CaP	484:486	We recently fabricated heparin-modified calcium phosphate (CaP) nanoparticles loaded with a large number of IO nanocrystals via coprecipitation from a supersaturated CaP solution supplemented with heparin and ferucarbotran (IO nanocrystals coated with carboxydextran).
34057430	1	1	theme	leading	178:184	arg1	cause					186:190	a leading cause	176:190	a leading cause of death worldwide	176:209	Atherosclerosis is a macrophage-related inflammatory disease that remains a leading cause of death worldwide.
34057430	8	2	theme	nanocrystals	1571:1582	arg1	agent					1559:1563	a delivery agent	1548:1563	a delivery agent of IO nanocrystals to macrophages in arterial lesions	1548:1617	Our nanoparticles have potential as a delivery agent of IO nanocrystals to macrophages in arterial lesions.
34057430	8	2	theme	nanocrystals	1571:1582	arg1	potential					1535:1543	potential	1535:1543	potential	1535:1543	Our nanoparticles have potential as a delivery agent of IO nanocrystals to macrophages in arterial lesions.
34057430	7	3	theme	free	1492:1495	arg1	ferucarbotran					1497:1509	free ferucarbotran	1492:1509	free ferucarbotran	1492:1509	A preliminaryin vivostudy showed that the nanoparticles injected intravenously into mice delivered more IO nanocrystals to macrophage-rich carotid arterial lesions than free ferucarbotran.
34057430	5	4	theme	due	1124:1126	arg1	particles					1114:1122	particles	1114:1122	particles due to the surface modification with heparin	1114:1167	The increase in nanoparticle IO content caused a decrease in particle diameter without impairing its dispersibility; the nanoparticles remained dispersed in water for up to 2 h due to electrostatic repulsion between particles due to the surface modification with heparin.
34057430	5	5	theme	particle	959:966	arg1	diameter					968:975	particle diameter	959:975	particle diameter	959:975	The increase in nanoparticle IO content caused a decrease in particle diameter without impairing its dispersibility; the nanoparticles remained dispersed in water for up to 2 h due to electrostatic repulsion between particles due to the surface modification with heparin.
34057430	3	6	theme	IO	533:534	arg1	nanocrystals					536:547	IO nanocrystals	533:547	IO nanocrystals	533:547	We recently fabricated heparin-modified calcium phosphate (CaP) nanoparticles loaded with a large number of IO nanocrystals via coprecipitation from a supersaturated CaP solution supplemented with heparin and ferucarbotran (IO nanocrystals coated with carboxydextran).
34057430	6	7	theme	significant	1297:1307	arg1	cytotoxicity					1309:1320	significant cytotoxicity	1297:1320	significant cytotoxicity	1297:1320	The nanoparticles were more effectively taken up by murine RAW264.7 macrophages compared to free ferucarbotran without showing significant cytotoxicity.
34057430	7	8	theme	macrophage-rich	1446:1460	arg1	lesions					1479:1485	macrophage-rich carotid arterial lesions	1446:1485	macrophage-rich carotid arterial lesions than free ferucarbotran	1446:1509	A preliminaryin vivostudy showed that the nanoparticles injected intravenously into mice delivered more IO nanocrystals to macrophage-rich carotid arterial lesions than free ferucarbotran.
34057430	7	9	dep	macrophage-rich	1446:1460	arg1	arterial					1470:1477	arterial	1470:1477	arterial	1470:1477	A preliminaryin vivostudy showed that the nanoparticles injected intravenously into mice delivered more IO nanocrystals to macrophage-rich carotid arterial lesions than free ferucarbotran.
34057430	6	10	theme	free	1262:1265	arg1	ferucarbotran					1267:1279	free ferucarbotran	1262:1279	free ferucarbotran	1262:1279	The nanoparticles were more effectively taken up by murine RAW264.7 macrophages compared to free ferucarbotran without showing significant cytotoxicity.
34057430	6	11	theme	RAW264.7	1229:1236	arg1	macrophages					1238:1248	murine RAW264.7 macrophages	1222:1248	murine RAW264.7 macrophages compared to free ferucarbotran without showing significant cytotoxicity	1222:1320	The nanoparticles were more effectively taken up by murine RAW264.7 macrophages compared to free ferucarbotran without showing significant cytotoxicity.
34057430	3	12	theme	supersaturated	576:589	arg1	solution					595:602	a supersaturated CaP solution	574:602	a supersaturated CaP solution supplemented with heparin and ferucarbotran (IO nanocrystals coated with carboxydextran)	574:691	We recently fabricated heparin-modified calcium phosphate (CaP) nanoparticles loaded with a large number of IO nanocrystals via coprecipitation from a supersaturated CaP solution supplemented with heparin and ferucarbotran (IO nanocrystals coated with carboxydextran).
34057430	3	12	theme	supersaturated	576:589	arg1	nanocrystals					652:663	IO nanocrystals	649:663	IO nanocrystals coated with carboxydextran	649:690	We recently fabricated heparin-modified calcium phosphate (CaP) nanoparticles loaded with a large number of IO nanocrystals via coprecipitation from a supersaturated CaP solution supplemented with heparin and ferucarbotran (IO nanocrystals coated with carboxydextran).
34057430	5	13	from	decrease	947:954	arg1	diameter					968:975	particle diameter	959:975	particle diameter	959:975	The increase in nanoparticle IO content caused a decrease in particle diameter without impairing its dispersibility; the nanoparticles remained dispersed in water for up to 2 h due to electrostatic repulsion between particles due to the surface modification with heparin.
34057430	2	14	theme	contrast	300:307	arg1	agents					309:314	contrast agents	300:314	contrast agents	300:314	Magnetic iron oxide (IO) nanocrystals are clinically used as magnetic resonance imaging contrast agents and their application as a detection agent for macrophages in arterial lesions has been studied extensively.
34057430	5	15	theme	IO	927:928	arg1	content					930:936	nanoparticle IO content	914:936	nanoparticle IO content	914:936	The increase in nanoparticle IO content caused a decrease in particle diameter without impairing its dispersibility; the nanoparticles remained dispersed in water for up to 2 h due to electrostatic repulsion between particles due to the surface modification with heparin.
34057430	3	16	theme	large	517:521	arg1	number					523:528	a large number	515:528	a large number of IO nanocrystals	515:547	We recently fabricated heparin-modified calcium phosphate (CaP) nanoparticles loaded with a large number of IO nanocrystals via coprecipitation from a supersaturated CaP solution supplemented with heparin and ferucarbotran (IO nanocrystals coated with carboxydextran).
34057430	2	17	used	used	265:268	arg2	resonance					282:290	magnetic resonance	273:290	magnetic resonance imaging contrast agents	273:314	Magnetic iron oxide (IO) nanocrystals are clinically used as magnetic resonance imaging contrast agents and their application as a detection agent for macrophages in arterial lesions has been studied extensively.
34057430	2	17	used	used	265:268	arg2	nanocrystals					237:248	Magnetic iron oxide (IO) nanocrystals	212:248	Magnetic iron oxide (IO) nanocrystals	212:248	Magnetic iron oxide (IO) nanocrystals are clinically used as magnetic resonance imaging contrast agents and their application as a detection agent for macrophages in arterial lesions has been studied extensively.
34057430	3	18	theme	phosphate	473:481	arg1	nanoparticles					489:501	heparin-modified calcium phosphate (CaP) nanoparticles	448:501	heparin-modified calcium phosphate (CaP) nanoparticles loaded with a large number of IO nanocrystals via coprecipitation from a supersaturated CaP solution supplemented with heparin and ferucarbotran (IO nanocrystals coated with carboxydextran)	448:691	We recently fabricated heparin-modified calcium phosphate (CaP) nanoparticles loaded with a large number of IO nanocrystals via coprecipitation from a supersaturated CaP solution supplemented with heparin and ferucarbotran (IO nanocrystals coated with carboxydextran).
34057430	1	19	theme	death	195:199	arg1	worldwide					201:209	death worldwide	195:209	death worldwide	195:209	Atherosclerosis is a macrophage-related inflammatory disease that remains a leading cause of death worldwide.
34057430	3	20	dep	supplemented	604:615	arg1	nanocrystals					652:663	IO nanocrystals	649:663	IO nanocrystals coated with carboxydextran	649:690	We recently fabricated heparin-modified calcium phosphate (CaP) nanoparticles loaded with a large number of IO nanocrystals via coprecipitation from a supersaturated CaP solution supplemented with heparin and ferucarbotran (IO nanocrystals coated with carboxydextran).
34057430	3	20	dep	supplemented	604:615	arg1	solution					595:602	a supersaturated CaP solution	574:602	a supersaturated CaP solution supplemented with heparin and ferucarbotran (IO nanocrystals coated with carboxydextran)	574:691	We recently fabricated heparin-modified calcium phosphate (CaP) nanoparticles loaded with a large number of IO nanocrystals via coprecipitation from a supersaturated CaP solution supplemented with heparin and ferucarbotran (IO nanocrystals coated with carboxydextran).
34057430	8	21	theme	delivery	1550:1557	arg1	agent					1559:1563	a delivery agent	1548:1563	a delivery agent of IO nanocrystals to macrophages in arterial lesions	1548:1617	Our nanoparticles have potential as a delivery agent of IO nanocrystals to macrophages in arterial lesions.
34057430	8	21	theme	delivery	1550:1557	arg1	potential					1535:1543	potential	1535:1543	potential	1535:1543	Our nanoparticles have potential as a delivery agent of IO nanocrystals to macrophages in arterial lesions.
34057430	2	22	from	macrophages	363:373	arg1	lesions					387:393	arterial lesions	378:393	arterial lesions	378:393	Magnetic iron oxide (IO) nanocrystals are clinically used as magnetic resonance imaging contrast agents and their application as a detection agent for macrophages in arterial lesions has been studied extensively.
34057430	4	23	theme	heparin-modified	768:783	arg1	nanoparticles					802:814	the heparin-modified IO-CaP composite nanoparticles	764:814	the heparin-modified IO-CaP composite nanoparticles	764:814	In this study, we further increased the content of IO nanocrystals in the heparin-modified IO-CaP composite nanoparticles by increasing the ferucarbotran concentration in the supersaturated CaP solution.
34057430	1	24	theme	worldwide	201:209	arg1	cause					186:190	a leading cause	176:190	a leading cause of death worldwide	176:209	Atherosclerosis is a macrophage-related inflammatory disease that remains a leading cause of death worldwide.
34057430	6	25	theme	murine	1222:1227	arg1	macrophages					1238:1248	murine RAW264.7 macrophages	1222:1248	murine RAW264.7 macrophages compared to free ferucarbotran without showing significant cytotoxicity	1222:1320	The nanoparticles were more effectively taken up by murine RAW264.7 macrophages compared to free ferucarbotran without showing significant cytotoxicity.
34057430	5	26	with	modification	1143:1154	arg1	heparin					1161:1167	heparin	1161:1167	heparin	1161:1167	The increase in nanoparticle IO content caused a decrease in particle diameter without impairing its dispersibility; the nanoparticles remained dispersed in water for up to 2 h due to electrostatic repulsion between particles due to the surface modification with heparin.
34057430	3	27	theme	calcium	465:471	arg1	phosphate					473:481	heparin-modified calcium phosphate	448:481	heparin-modified calcium phosphate (CaP) nanoparticles loaded with a large number of IO nanocrystals via coprecipitation from a supersaturated CaP solution supplemented with heparin and ferucarbotran (IO nanocrystals coated with carboxydextran)	448:691	We recently fabricated heparin-modified calcium phosphate (CaP) nanoparticles loaded with a large number of IO nanocrystals via coprecipitation from a supersaturated CaP solution supplemented with heparin and ferucarbotran (IO nanocrystals coated with carboxydextran).
34057430	3	27	theme	calcium	465:471	arg1	CaP					484:486	CaP	484:486	CaP	484:486	We recently fabricated heparin-modified calcium phosphate (CaP) nanoparticles loaded with a large number of IO nanocrystals via coprecipitation from a supersaturated CaP solution supplemented with heparin and ferucarbotran (IO nanocrystals coated with carboxydextran).
34057430	0	28	theme	iron	16:19	arg1	composite					45:53	iron oxide-calcium phosphate composite	16:53	iron oxide-calcium phosphate composite	16:53	In vivostudy of iron oxide-calcium phosphate composite nanoparticles for delivery to atherosclerosis.
34057430	8	29	theme	IO	1568:1569	arg1	nanocrystals					1571:1582	IO nanocrystals	1568:1582	IO nanocrystals to macrophages in arterial lesions	1568:1617	Our nanoparticles have potential as a delivery agent of IO nanocrystals to macrophages in arterial lesions.
34057430	3	30	theme	CaP	591:593	arg1	solution					595:602	a supersaturated CaP solution	574:602	a supersaturated CaP solution supplemented with heparin and ferucarbotran (IO nanocrystals coated with carboxydextran)	574:691	We recently fabricated heparin-modified calcium phosphate (CaP) nanoparticles loaded with a large number of IO nanocrystals via coprecipitation from a supersaturated CaP solution supplemented with heparin and ferucarbotran (IO nanocrystals coated with carboxydextran).
34057430	3	30	theme	CaP	591:593	arg1	nanocrystals					652:663	IO nanocrystals	649:663	IO nanocrystals coated with carboxydextran	649:690	We recently fabricated heparin-modified calcium phosphate (CaP) nanoparticles loaded with a large number of IO nanocrystals via coprecipitation from a supersaturated CaP solution supplemented with heparin and ferucarbotran (IO nanocrystals coated with carboxydextran).
34057430	5	31	dep	2	1071:1071	arg1	to					1068:1069	to	1068:1069	to	1068:1069	The increase in nanoparticle IO content caused a decrease in particle diameter without impairing its dispersibility; the nanoparticles remained dispersed in water for up to 2 h due to electrostatic repulsion between particles due to the surface modification with heparin.
34057430	4	32	theme	IO-CaP	785:790	arg1	nanoparticles					802:814	the heparin-modified IO-CaP composite nanoparticles	764:814	the heparin-modified IO-CaP composite nanoparticles	764:814	In this study, we further increased the content of IO nanocrystals in the heparin-modified IO-CaP composite nanoparticles by increasing the ferucarbotran concentration in the supersaturated CaP solution.
34057430	7	33	theme	IO	1427:1428	arg1	nanocrystals					1430:1441	more IO nanocrystals	1422:1441	more IO nanocrystals	1422:1441	A preliminaryin vivostudy showed that the nanoparticles injected intravenously into mice delivered more IO nanocrystals to macrophage-rich carotid arterial lesions than free ferucarbotran.
34057430	0	34	theme	phosphate	35:43	arg1	composite					45:53	iron oxide-calcium phosphate composite	16:53	iron oxide-calcium phosphate composite	16:53	In vivostudy of iron oxide-calcium phosphate composite nanoparticles for delivery to atherosclerosis.
34057430	5	35	theme	surface	1135:1141	arg1	modification					1143:1154	the surface modification	1131:1154	the surface modification with heparin	1131:1167	The increase in nanoparticle IO content caused a decrease in particle diameter without impairing its dispersibility; the nanoparticles remained dispersed in water for up to 2 h due to electrostatic repulsion between particles due to the surface modification with heparin.
34057430	4	36	theme	CaP	884:886	arg1	solution					888:895	the supersaturated CaP solution	865:895	the supersaturated CaP solution	865:895	In this study, we further increased the content of IO nanocrystals in the heparin-modified IO-CaP composite nanoparticles by increasing the ferucarbotran concentration in the supersaturated CaP solution.
34057430	0	37	theme	oxide-calcium	21:33	arg1	composite					45:53	iron oxide-calcium phosphate composite	16:53	iron oxide-calcium phosphate composite	16:53	In vivostudy of iron oxide-calcium phosphate composite nanoparticles for delivery to atherosclerosis.
34057430	4	38	theme	supersaturated	869:882	arg1	solution					888:895	the supersaturated CaP solution	865:895	the supersaturated CaP solution	865:895	In this study, we further increased the content of IO nanocrystals in the heparin-modified IO-CaP composite nanoparticles by increasing the ferucarbotran concentration in the supersaturated CaP solution.
34057430	5	39	theme	nanoparticle	914:925	arg1	content					930:936	nanoparticle IO content	914:936	nanoparticle IO content	914:936	The increase in nanoparticle IO content caused a decrease in particle diameter without impairing its dispersibility; the nanoparticles remained dispersed in water for up to 2 h due to electrostatic repulsion between particles due to the surface modification with heparin.
34057430	4	40	theme	composite	792:800	arg1	nanoparticles					802:814	the heparin-modified IO-CaP composite nanoparticles	764:814	the heparin-modified IO-CaP composite nanoparticles	764:814	In this study, we further increased the content of IO nanocrystals in the heparin-modified IO-CaP composite nanoparticles by increasing the ferucarbotran concentration in the supersaturated CaP solution.
34057430	8	41	contain	have	1530:1533	arg2	agent					1559:1563	a delivery agent	1548:1563	a delivery agent of IO nanocrystals to macrophages in arterial lesions	1548:1617	Our nanoparticles have potential as a delivery agent of IO nanocrystals to macrophages in arterial lesions.
34057430	8	41	contain	have	1530:1533	arg2	potential					1535:1543	potential	1535:1543	potential	1535:1543	Our nanoparticles have potential as a delivery agent of IO nanocrystals to macrophages in arterial lesions.
34057430	8	41	contain	have	1530:1533	arg1	nanoparticles					1516:1528	Our nanoparticles	1512:1528	Our nanoparticles	1512:1528	Our nanoparticles have potential as a delivery agent of IO nanocrystals to macrophages in arterial lesions.
34057430	8	42	from	macrophages	1587:1597	arg1	lesions					1611:1617	arterial lesions	1602:1617	arterial lesions	1602:1617	Our nanoparticles have potential as a delivery agent of IO nanocrystals to macrophages in arterial lesions.
34057430	5	43	theme	electrostatic	1082:1094	arg1	repulsion					1096:1104	electrostatic repulsion	1082:1104	electrostatic repulsion between particles due to the surface modification with heparin	1082:1167	The increase in nanoparticle IO content caused a decrease in particle diameter without impairing its dispersibility; the nanoparticles remained dispersed in water for up to 2 h due to electrostatic repulsion between particles due to the surface modification with heparin.
34057430	1	44	theme	macrophage-related	123:140	arg1	disease					155:161	a macrophage-related inflammatory disease	121:161	a macrophage-related inflammatory disease that remains a leading cause of death worldwide	121:209	Atherosclerosis is a macrophage-related inflammatory disease that remains a leading cause of death worldwide.
34057430	1	44	theme	macrophage-related	123:140	arg1	Atherosclerosis					102:116	Atherosclerosis	102:116	Atherosclerosis	102:116	Atherosclerosis is a macrophage-related inflammatory disease that remains a leading cause of death worldwide.
34057430	0	45	theme	composite	45:53	arg1	vivostudy					3:11	vivostudy	3:11	vivostudy of iron oxide-calcium phosphate composite	3:53	In vivostudy of iron oxide-calcium phosphate composite nanoparticles for delivery to atherosclerosis.
34057430	8	46	theme	arterial	1602:1609	arg1	lesions					1611:1617	arterial lesions	1602:1617	arterial lesions	1602:1617	Our nanoparticles have potential as a delivery agent of IO nanocrystals to macrophages in arterial lesions.
34057430	1	47	theme	inflammatory	142:153	arg1	disease					155:161	a macrophage-related inflammatory disease	121:161	a macrophage-related inflammatory disease that remains a leading cause of death worldwide	121:209	Atherosclerosis is a macrophage-related inflammatory disease that remains a leading cause of death worldwide.
34057430	1	47	theme	inflammatory	142:153	arg1	Atherosclerosis					102:116	Atherosclerosis	102:116	Atherosclerosis	102:116	Atherosclerosis is a macrophage-related inflammatory disease that remains a leading cause of death worldwide.
34057430	4	48	theme	IO	745:746	arg1	nanocrystals					748:759	IO nanocrystals	745:759	IO nanocrystals	745:759	In this study, we further increased the content of IO nanocrystals in the heparin-modified IO-CaP composite nanoparticles by increasing the ferucarbotran concentration in the supersaturated CaP solution.
34057430	2	49	theme	oxide	226:230	arg1	nanocrystals					237:248	Magnetic iron oxide (IO) nanocrystals	212:248	Magnetic iron oxide (IO) nanocrystals	212:248	Magnetic iron oxide (IO) nanocrystals are clinically used as magnetic resonance imaging contrast agents and their application as a detection agent for macrophages in arterial lesions has been studied extensively.
34057430	2	49	theme	oxide	226:230	arg1	resonance					282:290	magnetic resonance	273:290	magnetic resonance imaging contrast agents	273:314	Magnetic iron oxide (IO) nanocrystals are clinically used as magnetic resonance imaging contrast agents and their application as a detection agent for macrophages in arterial lesions has been studied extensively.
34057430	2	50	theme	magnetic	273:280	arg1	nanocrystals					237:248	Magnetic iron oxide (IO) nanocrystals	212:248	Magnetic iron oxide (IO) nanocrystals	212:248	Magnetic iron oxide (IO) nanocrystals are clinically used as magnetic resonance imaging contrast agents and their application as a detection agent for macrophages in arterial lesions has been studied extensively.
34057430	2	50	theme	magnetic	273:280	arg1	resonance					282:290	magnetic resonance	273:290	magnetic resonance imaging contrast agents	273:314	Magnetic iron oxide (IO) nanocrystals are clinically used as magnetic resonance imaging contrast agents and their application as a detection agent for macrophages in arterial lesions has been studied extensively.
34057430	5	51	from	increase	902:909	arg1	content					930:936	nanoparticle IO content	914:936	nanoparticle IO content	914:936	The increase in nanoparticle IO content caused a decrease in particle diameter without impairing its dispersibility; the nanoparticles remained dispersed in water for up to 2 h due to electrostatic repulsion between particles due to the surface modification with heparin.
34057430	2	52	theme	detection	343:351	arg1	agent					353:357	a detection agent	341:357	a detection agent for macrophages in arterial lesions	341:393	Magnetic iron oxide (IO) nanocrystals are clinically used as magnetic resonance imaging contrast agents and their application as a detection agent for macrophages in arterial lesions has been studied extensively.
34057430	3	53	from	solution	595:602	arg1	coprecipitation					553:567	coprecipitation	553:567	coprecipitation from a supersaturated CaP solution supplemented with heparin and ferucarbotran (IO nanocrystals coated with carboxydextran)	553:691	We recently fabricated heparin-modified calcium phosphate (CaP) nanoparticles loaded with a large number of IO nanocrystals via coprecipitation from a supersaturated CaP solution supplemented with heparin and ferucarbotran (IO nanocrystals coated with carboxydextran).
34057430	2	54	theme	iron	221:224	arg1	IO					233:234	IO	233:234	IO	233:234	Magnetic iron oxide (IO) nanocrystals are clinically used as magnetic resonance imaging contrast agents and their application as a detection agent for macrophages in arterial lesions has been studied extensively.
34057430	2	54	theme	iron	221:224	arg1	oxide					226:230	iron oxide	221:230	Magnetic iron oxide (IO) nanocrystals	212:248	Magnetic iron oxide (IO) nanocrystals are clinically used as magnetic resonance imaging contrast agents and their application as a detection agent for macrophages in arterial lesions has been studied extensively.
34057430	4	55	theme	ferucarbotran	834:846	arg1	concentration					848:860	the ferucarbotran concentration	830:860	the ferucarbotran concentration in the supersaturated CaP solution	830:895	In this study, we further increased the content of IO nanocrystals in the heparin-modified IO-CaP composite nanoparticles by increasing the ferucarbotran concentration in the supersaturated CaP solution.
34057430	4	56	theme	nanocrystals	748:759	arg1	content					734:740	the content	730:740	the content of IO nanocrystals in the heparin-modified IO-CaP composite nanoparticles	730:814	In this study, we further increased the content of IO nanocrystals in the heparin-modified IO-CaP composite nanoparticles by increasing the ferucarbotran concentration in the supersaturated CaP solution.
34057430	2	57	theme	Magnetic	212:219	arg1	nanocrystals					237:248	Magnetic iron oxide (IO) nanocrystals	212:248	Magnetic iron oxide (IO) nanocrystals	212:248	Magnetic iron oxide (IO) nanocrystals are clinically used as magnetic resonance imaging contrast agents and their application as a detection agent for macrophages in arterial lesions has been studied extensively.
34057430	2	57	theme	Magnetic	212:219	arg1	resonance					282:290	magnetic resonance	273:290	magnetic resonance imaging contrast agents	273:314	Magnetic iron oxide (IO) nanocrystals are clinically used as magnetic resonance imaging contrast agents and their application as a detection agent for macrophages in arterial lesions has been studied extensively.
34057430	3	58	theme	IO	649:650	arg1	nanocrystals					652:663	IO nanocrystals	649:663	IO nanocrystals coated with carboxydextran	649:690	We recently fabricated heparin-modified calcium phosphate (CaP) nanoparticles loaded with a large number of IO nanocrystals via coprecipitation from a supersaturated CaP solution supplemented with heparin and ferucarbotran (IO nanocrystals coated with carboxydextran).
34057430	3	58	theme	IO	649:650	arg1	solution					595:602	a supersaturated CaP solution	574:602	a supersaturated CaP solution supplemented with heparin and ferucarbotran (IO nanocrystals coated with carboxydextran)	574:691	We recently fabricated heparin-modified calcium phosphate (CaP) nanoparticles loaded with a large number of IO nanocrystals via coprecipitation from a supersaturated CaP solution supplemented with heparin and ferucarbotran (IO nanocrystals coated with carboxydextran).
34057430	7	59	theme	more	1422:1425	arg1	nanocrystals					1430:1441	more IO nanocrystals	1422:1441	more IO nanocrystals	1422:1441	A preliminaryin vivostudy showed that the nanoparticles injected intravenously into mice delivered more IO nanocrystals to macrophage-rich carotid arterial lesions than free ferucarbotran.
34057430	3	60	theme	nanocrystals	536:547	arg1	number					523:528	a large number	515:528	a large number of IO nanocrystals	515:547	We recently fabricated heparin-modified calcium phosphate (CaP) nanoparticles loaded with a large number of IO nanocrystals via coprecipitation from a supersaturated CaP solution supplemented with heparin and ferucarbotran (IO nanocrystals coated with carboxydextran).
34057430	7	61	theme	preliminaryin	1325:1337	arg1	vivostudy					1339:1347	A preliminaryin vivostudy	1323:1347	A preliminaryin vivostudy	1323:1347	A preliminaryin vivostudy showed that the nanoparticles injected intravenously into mice delivered more IO nanocrystals to macrophage-rich carotid arterial lesions than free ferucarbotran.
34057430	4	62	from	concentration	848:860	arg1	solution					888:895	the supersaturated CaP solution	865:895	the supersaturated CaP solution	865:895	In this study, we further increased the content of IO nanocrystals in the heparin-modified IO-CaP composite nanoparticles by increasing the ferucarbotran concentration in the supersaturated CaP solution.
34057430	2	63	theme	arterial	378:385	arg1	lesions					387:393	arterial lesions	378:393	arterial lesions	378:393	Magnetic iron oxide (IO) nanocrystals are clinically used as magnetic resonance imaging contrast agents and their application as a detection agent for macrophages in arterial lesions has been studied extensively.
34057430	4	64	from	content	734:740	arg1	nanoparticles					802:814	the heparin-modified IO-CaP composite nanoparticles	764:814	the heparin-modified IO-CaP composite nanoparticles	764:814	In this study, we further increased the content of IO nanocrystals in the heparin-modified IO-CaP composite nanoparticles by increasing the ferucarbotran concentration in the supersaturated CaP solution.
34440360	0	0	theme	Porcine	84:90	arg1	Ovaries					92:98	Bovine and Porcine Ovaries	73:98	Bovine and Porcine Ovaries	73:98	Hyaluronan and Collagen Are Prominent Extracellular Matrix Components in Bovine and Porcine Ovaries.
34440360	5	1	theme	species-specific	742:757	arg1	differences					759:769	species-specific differences	742:769	species-specific differences in collagen gene transcripts	742:798	Bovine ovaries had more collagen than porcine ovaries when assessed biochemically, and this was associated with species-specific differences in collagen gene transcripts: Col3a1 was predominant in cow ovaries while Col1a1 was predominant in pig ovaries.
34440360	7	2	theme	follicular	1058:1067	arg1	fluid					1069:1073	follicular fluid	1058:1073	follicular fluid	1058:1073	HA was distributed across three molecular weight ranges (<100 kDa, 100-300 kDa, and >300 kDa) in ovarian tissue and follicular fluid, with tissue having more >300 kDa HA than the other two ranges.
34440360	1	3	theme	major	137:141	arg1	matrix					119:124	The extracellular matrix	101:124	The extracellular matrix (ECM)	101:130	The extracellular matrix (ECM) is a major component of the ovarian stroma.
34440360	1	3	theme	major	137:141	arg1	component					143:151	a major component	135:151	a major component of the ovarian stroma	135:173	The extracellular matrix (ECM) is a major component of the ovarian stroma.
34440360	2	4	from	changes	275:281	arg1	human					300:304	human	300:304	human	300:304	Collagen and hyaluronan (HA) are critical ovarian stromal ECM molecules that undergo age-dependent changes in the mouse and human.
34440360	2	4	from	changes	275:281	arg1	mouse					290:294	mouse	290:294	mouse	290:294	Collagen and hyaluronan (HA) are critical ovarian stromal ECM molecules that undergo age-dependent changes in the mouse and human.
34440360	6	5	theme	more	901:904	arg1	HA					906:907	more HA	901:907	more HA	901:907	We also observed more HA in the porcine vs. bovine ovary.
34440360	5	6	theme	Bovine	630:635	arg1	ovaries					637:643	Bovine ovaries	630:643	Bovine ovaries	630:643	Bovine ovaries had more collagen than porcine ovaries when assessed biochemically, and this was associated with species-specific differences in collagen gene transcripts: Col3a1 was predominant in cow ovaries while Col1a1 was predominant in pig ovaries.
34440360	7	7	theme	other	1121:1125	arg1	ranges					1131:1136	the other two ranges	1117:1136	the other two ranges	1117:1136	HA was distributed across three molecular weight ranges (<100 kDa, 100-300 kDa, and >300 kDa) in ovarian tissue and follicular fluid, with tissue having more >300 kDa HA than the other two ranges.
34440360	7	8	dep	kDa	1105:1107	arg1	>300					1100:1103	>300	1100:1103	>300	1100:1103	HA was distributed across three molecular weight ranges (<100 kDa, 100-300 kDa, and >300 kDa) in ovarian tissue and follicular fluid, with tissue having more >300 kDa HA than the other two ranges.
34440360	7	9	contain	having	1088:1093	arg1	tissue					1081:1086	tissue	1081:1086	tissue having more >300 kDa HA than the other two ranges	1081:1136	HA was distributed across three molecular weight ranges (<100 kDa, 100-300 kDa, and >300 kDa) in ovarian tissue and follicular fluid, with tissue having more >300 kDa HA than the other two ranges.
34440360	7	9	contain	having	1088:1093	arg2	HA					1109:1110	more >300 kDa HA	1095:1110	more >300 kDa HA than the other two ranges	1095:1136	HA was distributed across three molecular weight ranges (<100 kDa, 100-300 kDa, and >300 kDa) in ovarian tissue and follicular fluid, with tissue having more >300 kDa HA than the other two ranges.
34440360	2	10	theme	age-dependent	261:273	arg1	changes					275:281	age-dependent changes	261:281	age-dependent changes in the mouse and human	261:304	Collagen and hyaluronan (HA) are critical ovarian stromal ECM molecules that undergo age-dependent changes in the mouse and human.
34440360	3	11	dep	investigated	492:503	arg1	organized					353:361	organized	353:361	organized in other mammalian species with reproductive characteristics similar to women such as cows and pigs	353:461	How these matrix components are regulated and organized in other mammalian species with reproductive characteristics similar to women such as cows and pigs, has not been systematically investigated.
34440360	3	11	dep	investigated	492:503	arg1	regulated					339:347	regulated	339:347	regulated	339:347	How these matrix components are regulated and organized in other mammalian species with reproductive characteristics similar to women such as cows and pigs, has not been systematically investigated.
34440360	0	12	from	Components	59:68	arg1	Ovaries					92:98	Bovine and Porcine Ovaries	73:98	Bovine and Porcine Ovaries	73:98	Hyaluronan and Collagen Are Prominent Extracellular Matrix Components in Bovine and Porcine Ovaries.
34440360	7	13	dep	HA	1109:1110	arg1	kDa					1105:1107	kDa	1105:1107	kDa	1105:1107	HA was distributed across three molecular weight ranges (<100 kDa, 100-300 kDa, and >300 kDa) in ovarian tissue and follicular fluid, with tissue having more >300 kDa HA than the other two ranges.
34440360	5	14	attach	predominant	812:822	arg1	ovaries					831:837	cow ovaries	827:837	cow ovaries	827:837	Bovine ovaries had more collagen than porcine ovaries when assessed biochemically, and this was associated with species-specific differences in collagen gene transcripts: Col3a1 was predominant in cow ovaries while Col1a1 was predominant in pig ovaries.
34440360	5	14	attach	predominant	812:822	arg2	Col3a1					801:806	Col3a1	801:806	Col3a1	801:806	Bovine ovaries had more collagen than porcine ovaries when assessed biochemically, and this was associated with species-specific differences in collagen gene transcripts: Col3a1 was predominant in cow ovaries while Col1a1 was predominant in pig ovaries.
34440360	8	15	theme	cow	1243:1245	arg1	ovaries					1247:1253	cow ovaries	1243:1253	cow ovaries	1243:1253	Transcripts for HA synthesis and degradation enzymes, Has3 and Hyal2, respectively, were predominant in cow ovaries, whereas Has2, Kiaa1199, and Tmem2 tended to be predominant in pig ovaries.
34440360	4	16	theme	biochemical	559:569	arg1	analyses					571:578	histological, molecular, and biochemical analyses	530:578	histological, molecular, and biochemical analyses	530:578	Therefore, we performed histological, molecular, and biochemical analyses to characterize collagen and HA in these animals.
34440360	5	17	from	predominant	812:822	arg1	ovaries					831:837	cow ovaries	827:837	cow ovaries	827:837	Bovine ovaries had more collagen than porcine ovaries when assessed biochemically, and this was associated with species-specific differences in collagen gene transcripts: Col3a1 was predominant in cow ovaries while Col1a1 was predominant in pig ovaries.
34440360	8	18	theme	degradation	1172:1182	arg1	Hyal2					1202:1206	Hyal2	1202:1206	Hyal2	1202:1206	Transcripts for HA synthesis and degradation enzymes, Has3 and Hyal2, respectively, were predominant in cow ovaries, whereas Has2, Kiaa1199, and Tmem2 tended to be predominant in pig ovaries.
34440360	8	18	theme	degradation	1172:1182	arg1	Has3					1193:1196	Has3	1193:1196	Has3	1193:1196	Transcripts for HA synthesis and degradation enzymes, Has3 and Hyal2, respectively, were predominant in cow ovaries, whereas Has2, Kiaa1199, and Tmem2 tended to be predominant in pig ovaries.
34440360	8	18	theme	degradation	1172:1182	arg1	enzymes					1184:1190	HA synthesis and degradation enzymes	1155:1190	HA synthesis and degradation enzymes	1155:1190	Transcripts for HA synthesis and degradation enzymes, Has3 and Hyal2, respectively, were predominant in cow ovaries, whereas Has2, Kiaa1199, and Tmem2 tended to be predominant in pig ovaries.
34440360	5	19	from	ovaries	875:881	arg1	predominant					856:866	predominant	856:866	predominant	856:866	Bovine ovaries had more collagen than porcine ovaries when assessed biochemically, and this was associated with species-specific differences in collagen gene transcripts: Col3a1 was predominant in cow ovaries while Col1a1 was predominant in pig ovaries.
34440360	3	20	with	species	382:388	arg1	characteristics					408:422	reproductive characteristics	395:422	reproductive characteristics similar to women such as cows and pigs	395:461	How these matrix components are regulated and organized in other mammalian species with reproductive characteristics similar to women such as cows and pigs, has not been systematically investigated.
34440360	1	21	theme	ovarian	160:166	arg1	stroma					168:173	the ovarian stroma	156:173	the ovarian stroma	156:173	The extracellular matrix (ECM) is a major component of the ovarian stroma.
34440360	3	22	theme	similar	424:430	arg1	characteristics					408:422	reproductive characteristics	395:422	reproductive characteristics similar to women such as cows and pigs	395:461	How these matrix components are regulated and organized in other mammalian species with reproductive characteristics similar to women such as cows and pigs, has not been systematically investigated.
34440360	0	23	theme	Matrix	52:57	arg1	Components					59:68	Prominent Extracellular Matrix Components	28:68	Prominent Extracellular Matrix Components in Bovine and Porcine Ovaries	28:98	Hyaluronan and Collagen Are Prominent Extracellular Matrix Components in Bovine and Porcine Ovaries.
34440360	7	24	theme	ovarian	1039:1045	arg1	tissue					1047:1052	ovarian tissue	1039:1052	ovarian tissue	1039:1052	HA was distributed across three molecular weight ranges (<100 kDa, 100-300 kDa, and >300 kDa) in ovarian tissue and follicular fluid, with tissue having more >300 kDa HA than the other two ranges.
34440360	9	25	dep	composition	1380:1390	arg1	the					1376:1378	the	1376:1378	the	1376:1378	Together, our findings have implications for the composition, organization, and regulation of the ovarian ECM in large mammalian species, including humans.
34440360	7	26	dep	ranges	991:996	arg1	kDa					1031:1033	>300 kDa	1026:1033	>300 kDa	1026:1033	HA was distributed across three molecular weight ranges (<100 kDa, 100-300 kDa, and >300 kDa) in ovarian tissue and follicular fluid, with tissue having more >300 kDa HA than the other two ranges.
34440360	7	26	dep	ranges	991:996	arg1	ranges					991:996	three molecular weight ranges	968:996	three molecular weight ranges (<100 kDa, 100-300 kDa, and >300 kDa) in ovarian tissue and follicular fluid	968:1073	HA was distributed across three molecular weight ranges (<100 kDa, 100-300 kDa, and >300 kDa) in ovarian tissue and follicular fluid, with tissue having more >300 kDa HA than the other two ranges.
34440360	7	26	dep	ranges	991:996	arg1	kDa					1004:1006	<100 kDa	999:1006	<100 kDa	999:1006	HA was distributed across three molecular weight ranges (<100 kDa, 100-300 kDa, and >300 kDa) in ovarian tissue and follicular fluid, with tissue having more >300 kDa HA than the other two ranges.
34440360	7	26	dep	ranges	991:996	arg1	kDa					1017:1019	100-300 kDa	1009:1019	100-300 kDa	1009:1019	HA was distributed across three molecular weight ranges (<100 kDa, 100-300 kDa, and >300 kDa) in ovarian tissue and follicular fluid, with tissue having more >300 kDa HA than the other two ranges.
34440360	8	27	theme	HA	1155:1156	arg1	synthesis					1158:1166	HA synthesis	1155:1166	HA synthesis	1155:1166	Transcripts for HA synthesis and degradation enzymes, Has3 and Hyal2, respectively, were predominant in cow ovaries, whereas Has2, Kiaa1199, and Tmem2 tended to be predominant in pig ovaries.
34440360	9	28	contain	have	1354:1357	arg2	implications					1359:1370	implications	1359:1370	implications for the composition, organization, and regulation of the ovarian ECM	1359:1439	Together, our findings have implications for the composition, organization, and regulation of the ovarian ECM in large mammalian species, including humans.
34440360	9	28	contain	have	1354:1357	arg1	findings					1345:1352	our findings	1341:1352	our findings	1341:1352	Together, our findings have implications for the composition, organization, and regulation of the ovarian ECM in large mammalian species, including humans.
34440360	9	29	theme	large	1444:1448	arg1	species					1460:1466	large mammalian species	1444:1466	large mammalian species	1444:1466	Together, our findings have implications for the composition, organization, and regulation of the ovarian ECM in large mammalian species, including humans.
34440360	9	29	theme	large	1444:1448	arg1	humans					1479:1484	humans	1479:1484	humans	1479:1484	Together, our findings have implications for the composition, organization, and regulation of the ovarian ECM in large mammalian species, including humans.
34440360	3	30	theme	reproductive	395:406	arg1	characteristics					408:422	reproductive characteristics	395:422	reproductive characteristics similar to women such as cows and pigs	395:461	How these matrix components are regulated and organized in other mammalian species with reproductive characteristics similar to women such as cows and pigs, has not been systematically investigated.
34440360	4	31	theme	histological	530:541	arg1	analyses					571:578	histological, molecular, and biochemical analyses	530:578	histological, molecular, and biochemical analyses	530:578	Therefore, we performed histological, molecular, and biochemical analyses to characterize collagen and HA in these animals.
34440360	8	32	theme	synthesis	1158:1166	arg1	Hyal2					1202:1206	Hyal2	1202:1206	Hyal2	1202:1206	Transcripts for HA synthesis and degradation enzymes, Has3 and Hyal2, respectively, were predominant in cow ovaries, whereas Has2, Kiaa1199, and Tmem2 tended to be predominant in pig ovaries.
34440360	8	32	theme	synthesis	1158:1166	arg1	Has3					1193:1196	Has3	1193:1196	Has3	1193:1196	Transcripts for HA synthesis and degradation enzymes, Has3 and Hyal2, respectively, were predominant in cow ovaries, whereas Has2, Kiaa1199, and Tmem2 tended to be predominant in pig ovaries.
34440360	8	32	theme	synthesis	1158:1166	arg1	enzymes					1184:1190	HA synthesis and degradation enzymes	1155:1190	HA synthesis and degradation enzymes	1155:1190	Transcripts for HA synthesis and degradation enzymes, Has3 and Hyal2, respectively, were predominant in cow ovaries, whereas Has2, Kiaa1199, and Tmem2 tended to be predominant in pig ovaries.
34440360	8	33	from	predominant	1228:1238	arg1	ovaries					1247:1253	cow ovaries	1243:1253	cow ovaries	1243:1253	Transcripts for HA synthesis and degradation enzymes, Has3 and Hyal2, respectively, were predominant in cow ovaries, whereas Has2, Kiaa1199, and Tmem2 tended to be predominant in pig ovaries.
34440360	9	34	theme	mammalian	1450:1458	arg1	species					1460:1466	large mammalian species	1444:1466	large mammalian species	1444:1466	Together, our findings have implications for the composition, organization, and regulation of the ovarian ECM in large mammalian species, including humans.
34440360	9	34	theme	mammalian	1450:1458	arg1	humans					1479:1484	humans	1479:1484	humans	1479:1484	Together, our findings have implications for the composition, organization, and regulation of the ovarian ECM in large mammalian species, including humans.
34440360	8	35	from	ovaries	1322:1328	arg1	predominant					1303:1313	predominant	1303:1313	predominant	1303:1313	Transcripts for HA synthesis and degradation enzymes, Has3 and Hyal2, respectively, were predominant in cow ovaries, whereas Has2, Kiaa1199, and Tmem2 tended to be predominant in pig ovaries.
34440360	1	36	theme	stroma	168:173	arg1	matrix					119:124	The extracellular matrix	101:124	The extracellular matrix (ECM)	101:130	The extracellular matrix (ECM) is a major component of the ovarian stroma.
34440360	1	36	theme	stroma	168:173	arg1	component					143:151	a major component	135:151	a major component of the ovarian stroma	135:173	The extracellular matrix (ECM) is a major component of the ovarian stroma.
34440360	4	37	theme	molecular	544:552	arg1	analyses					571:578	histological, molecular, and biochemical analyses	530:578	histological, molecular, and biochemical analyses	530:578	Therefore, we performed histological, molecular, and biochemical analyses to characterize collagen and HA in these animals.
34440360	9	38	theme	ECM	1437:1439	arg1	organization					1393:1404	organization	1393:1404	organization	1393:1404	Together, our findings have implications for the composition, organization, and regulation of the ovarian ECM in large mammalian species, including humans.
34440360	9	38	theme	ECM	1437:1439	arg1	regulation					1411:1420	regulation	1411:1420	regulation	1411:1420	Together, our findings have implications for the composition, organization, and regulation of the ovarian ECM in large mammalian species, including humans.
34440360	9	38	theme	ECM	1437:1439	arg1	composition					1380:1390	composition	1380:1390	composition	1380:1390	Together, our findings have implications for the composition, organization, and regulation of the ovarian ECM in large mammalian species, including humans.
34440360	1	39	theme	extracellular	105:117	arg1	ECM					127:129	ECM	127:129	ECM	127:129	The extracellular matrix (ECM) is a major component of the ovarian stroma.
34440360	1	39	theme	extracellular	105:117	arg1	matrix					119:124	The extracellular matrix	101:124	The extracellular matrix (ECM)	101:130	The extracellular matrix (ECM) is a major component of the ovarian stroma.
34440360	1	39	theme	extracellular	105:117	arg1	component					143:151	a major component	135:151	a major component of the ovarian stroma	135:173	The extracellular matrix (ECM) is a major component of the ovarian stroma.
34440360	5	40	theme	cow	827:829	arg1	ovaries					831:837	cow ovaries	827:837	cow ovaries	827:837	Bovine ovaries had more collagen than porcine ovaries when assessed biochemically, and this was associated with species-specific differences in collagen gene transcripts: Col3a1 was predominant in cow ovaries while Col1a1 was predominant in pig ovaries.
34440360	0	41	theme	Extracellular	38:50	arg1	Components					59:68	Prominent Extracellular Matrix Components	28:68	Prominent Extracellular Matrix Components in Bovine and Porcine Ovaries	28:98	Hyaluronan and Collagen Are Prominent Extracellular Matrix Components in Bovine and Porcine Ovaries.
34440360	6	42	theme	bovine	928:933	arg1	ovary					935:939	the porcine vs. bovine ovary	912:939	ovary	935:939	We also observed more HA in the porcine vs. bovine ovary.
34440360	8	43	from	predominant	1303:1313	arg1	ovaries					1322:1328	pig ovaries	1318:1328	pig ovaries	1318:1328	Transcripts for HA synthesis and degradation enzymes, Has3 and Hyal2, respectively, were predominant in cow ovaries, whereas Has2, Kiaa1199, and Tmem2 tended to be predominant in pig ovaries.
34440360	7	44	from	ranges	991:996	arg1	tissue					1047:1052	ovarian tissue	1039:1052	ovarian tissue	1039:1052	HA was distributed across three molecular weight ranges (<100 kDa, 100-300 kDa, and >300 kDa) in ovarian tissue and follicular fluid, with tissue having more >300 kDa HA than the other two ranges.
34440360	7	44	from	ranges	991:996	arg1	fluid					1069:1073	follicular fluid	1058:1073	follicular fluid	1058:1073	HA was distributed across three molecular weight ranges (<100 kDa, 100-300 kDa, and >300 kDa) in ovarian tissue and follicular fluid, with tissue having more >300 kDa HA than the other two ranges.
34440360	0	45	theme	Prominent	28:36	arg1	Components					59:68	Prominent Extracellular Matrix Components	28:68	Prominent Extracellular Matrix Components in Bovine and Porcine Ovaries	28:98	Hyaluronan and Collagen Are Prominent Extracellular Matrix Components in Bovine and Porcine Ovaries.
34440360	5	46	theme	collagen	774:781	arg1	transcripts					788:798	collagen gene transcripts	774:798	collagen gene transcripts	774:798	Bovine ovaries had more collagen than porcine ovaries when assessed biochemically, and this was associated with species-specific differences in collagen gene transcripts: Col3a1 was predominant in cow ovaries while Col1a1 was predominant in pig ovaries.
34440360	5	47	from	ovaries	831:837	arg1	predominant					812:822	predominant	812:822	predominant	812:822	Bovine ovaries had more collagen than porcine ovaries when assessed biochemically, and this was associated with species-specific differences in collagen gene transcripts: Col3a1 was predominant in cow ovaries while Col1a1 was predominant in pig ovaries.
34440360	3	48	theme	matrix	317:322	arg1	components					324:333	these matrix components	311:333	these matrix components	311:333	How these matrix components are regulated and organized in other mammalian species with reproductive characteristics similar to women such as cows and pigs, has not been systematically investigated.
34440360	3	49	theme	other	366:370	arg1	species					382:388	other mammalian species	366:388	other mammalian species with reproductive characteristics similar to women such as cows and pigs	366:461	How these matrix components are regulated and organized in other mammalian species with reproductive characteristics similar to women such as cows and pigs, has not been systematically investigated.
34440360	6	50	theme	porcine	916:922	arg1	ovary					935:939	the porcine vs. bovine ovary	912:939	ovary	935:939	We also observed more HA in the porcine vs. bovine ovary.
34440360	5	51	theme	more	649:652	arg1	collagen					654:661	more collagen	649:661	more collagen	649:661	Bovine ovaries had more collagen than porcine ovaries when assessed biochemically, and this was associated with species-specific differences in collagen gene transcripts: Col3a1 was predominant in cow ovaries while Col1a1 was predominant in pig ovaries.
34440360	5	52	attach	predominant	856:866	arg2	Col1a1					845:850	Col1a1	845:850	Col1a1	845:850	Bovine ovaries had more collagen than porcine ovaries when assessed biochemically, and this was associated with species-specific differences in collagen gene transcripts: Col3a1 was predominant in cow ovaries while Col1a1 was predominant in pig ovaries.
34440360	5	52	attach	predominant	856:866	arg1	ovaries					875:881	pig ovaries	871:881	pig ovaries	871:881	Bovine ovaries had more collagen than porcine ovaries when assessed biochemically, and this was associated with species-specific differences in collagen gene transcripts: Col3a1 was predominant in cow ovaries while Col1a1 was predominant in pig ovaries.
34440360	3	53	theme	mammalian	372:380	arg1	species					382:388	other mammalian species	366:388	other mammalian species with reproductive characteristics similar to women such as cows and pigs	366:461	How these matrix components are regulated and organized in other mammalian species with reproductive characteristics similar to women such as cows and pigs, has not been systematically investigated.
34440360	8	54	attach	predominant	1228:1238	arg1	ovaries					1247:1253	cow ovaries	1243:1253	cow ovaries	1243:1253	Transcripts for HA synthesis and degradation enzymes, Has3 and Hyal2, respectively, were predominant in cow ovaries, whereas Has2, Kiaa1199, and Tmem2 tended to be predominant in pig ovaries.
34440360	8	54	attach	predominant	1228:1238	arg2	Transcripts					1139:1149	Transcripts	1139:1149	Transcripts for HA synthesis and degradation enzymes, Has3 and Hyal2	1139:1206	Transcripts for HA synthesis and degradation enzymes, Has3 and Hyal2, respectively, were predominant in cow ovaries, whereas Has2, Kiaa1199, and Tmem2 tended to be predominant in pig ovaries.
34440360	7	55	theme	molecular	974:982	arg1	kDa					1031:1033	>300 kDa	1026:1033	>300 kDa	1026:1033	HA was distributed across three molecular weight ranges (<100 kDa, 100-300 kDa, and >300 kDa) in ovarian tissue and follicular fluid, with tissue having more >300 kDa HA than the other two ranges.
34440360	7	55	theme	molecular	974:982	arg1	ranges					991:996	three molecular weight ranges	968:996	three molecular weight ranges (<100 kDa, 100-300 kDa, and >300 kDa) in ovarian tissue and follicular fluid	968:1073	HA was distributed across three molecular weight ranges (<100 kDa, 100-300 kDa, and >300 kDa) in ovarian tissue and follicular fluid, with tissue having more >300 kDa HA than the other two ranges.
34440360	7	55	theme	molecular	974:982	arg1	kDa					1004:1006	<100 kDa	999:1006	<100 kDa	999:1006	HA was distributed across three molecular weight ranges (<100 kDa, 100-300 kDa, and >300 kDa) in ovarian tissue and follicular fluid, with tissue having more >300 kDa HA than the other two ranges.
34440360	7	55	theme	molecular	974:982	arg1	kDa					1017:1019	100-300 kDa	1009:1019	100-300 kDa	1009:1019	HA was distributed across three molecular weight ranges (<100 kDa, 100-300 kDa, and >300 kDa) in ovarian tissue and follicular fluid, with tissue having more >300 kDa HA than the other two ranges.
34440360	8	56	theme	pig	1318:1320	arg1	ovaries					1322:1328	pig ovaries	1318:1328	pig ovaries	1318:1328	Transcripts for HA synthesis and degradation enzymes, Has3 and Hyal2, respectively, were predominant in cow ovaries, whereas Has2, Kiaa1199, and Tmem2 tended to be predominant in pig ovaries.
34440360	5	57	from	differences	759:769	arg1	transcripts					788:798	collagen gene transcripts	774:798	collagen gene transcripts	774:798	Bovine ovaries had more collagen than porcine ovaries when assessed biochemically, and this was associated with species-specific differences in collagen gene transcripts: Col3a1 was predominant in cow ovaries while Col1a1 was predominant in pig ovaries.
34440360	2	58	theme	ECM	234:236	arg1	Collagen					176:183	Collagen	176:183	Collagen	176:183	Collagen and hyaluronan (HA) are critical ovarian stromal ECM molecules that undergo age-dependent changes in the mouse and human.
34440360	2	58	theme	ECM	234:236	arg1	molecules					238:246	critical ovarian stromal ECM molecules	209:246	critical ovarian stromal ECM molecules that undergo age-dependent changes in the mouse and human	209:304	Collagen and hyaluronan (HA) are critical ovarian stromal ECM molecules that undergo age-dependent changes in the mouse and human.
34440360	2	58	theme	ECM	234:236	arg1	hyaluronan					189:198	hyaluronan	189:198	hyaluronan (HA)	189:203	Collagen and hyaluronan (HA) are critical ovarian stromal ECM molecules that undergo age-dependent changes in the mouse and human.
34440360	7	59	theme	weight	984:989	arg1	kDa					1031:1033	>300 kDa	1026:1033	>300 kDa	1026:1033	HA was distributed across three molecular weight ranges (<100 kDa, 100-300 kDa, and >300 kDa) in ovarian tissue and follicular fluid, with tissue having more >300 kDa HA than the other two ranges.
34440360	7	59	theme	weight	984:989	arg1	ranges					991:996	three molecular weight ranges	968:996	three molecular weight ranges (<100 kDa, 100-300 kDa, and >300 kDa) in ovarian tissue and follicular fluid	968:1073	HA was distributed across three molecular weight ranges (<100 kDa, 100-300 kDa, and >300 kDa) in ovarian tissue and follicular fluid, with tissue having more >300 kDa HA than the other two ranges.
34440360	7	59	theme	weight	984:989	arg1	kDa					1004:1006	<100 kDa	999:1006	<100 kDa	999:1006	HA was distributed across three molecular weight ranges (<100 kDa, 100-300 kDa, and >300 kDa) in ovarian tissue and follicular fluid, with tissue having more >300 kDa HA than the other two ranges.
34440360	7	59	theme	weight	984:989	arg1	kDa					1017:1019	100-300 kDa	1009:1019	100-300 kDa	1009:1019	HA was distributed across three molecular weight ranges (<100 kDa, 100-300 kDa, and >300 kDa) in ovarian tissue and follicular fluid, with tissue having more >300 kDa HA than the other two ranges.
34440360	2	60	dep	mouse	290:294	arg1	the					286:288	the	286:288	the	286:288	Collagen and hyaluronan (HA) are critical ovarian stromal ECM molecules that undergo age-dependent changes in the mouse and human.
34440360	5	61	contain	had	645:647	arg1	ovaries					637:643	Bovine ovaries	630:643	Bovine ovaries	630:643	Bovine ovaries had more collagen than porcine ovaries when assessed biochemically, and this was associated with species-specific differences in collagen gene transcripts: Col3a1 was predominant in cow ovaries while Col1a1 was predominant in pig ovaries.
34440360	5	61	contain	had	645:647	arg2	collagen					654:661	more collagen	649:661	more collagen	649:661	Bovine ovaries had more collagen than porcine ovaries when assessed biochemically, and this was associated with species-specific differences in collagen gene transcripts: Col3a1 was predominant in cow ovaries while Col1a1 was predominant in pig ovaries.
34440360	5	62	theme	porcine	668:674	arg1	ovaries					676:682	porcine ovaries	668:682	porcine ovaries	668:682	Bovine ovaries had more collagen than porcine ovaries when assessed biochemically, and this was associated with species-specific differences in collagen gene transcripts: Col3a1 was predominant in cow ovaries while Col1a1 was predominant in pig ovaries.
34440360	2	63	theme	stromal	226:232	arg1	Collagen					176:183	Collagen	176:183	Collagen	176:183	Collagen and hyaluronan (HA) are critical ovarian stromal ECM molecules that undergo age-dependent changes in the mouse and human.
34440360	2	63	theme	stromal	226:232	arg1	molecules					238:246	critical ovarian stromal ECM molecules	209:246	critical ovarian stromal ECM molecules that undergo age-dependent changes in the mouse and human	209:304	Collagen and hyaluronan (HA) are critical ovarian stromal ECM molecules that undergo age-dependent changes in the mouse and human.
34440360	2	63	theme	stromal	226:232	arg1	hyaluronan					189:198	hyaluronan	189:198	hyaluronan (HA)	189:203	Collagen and hyaluronan (HA) are critical ovarian stromal ECM molecules that undergo age-dependent changes in the mouse and human.
34440360	5	64	from	predominant	856:866	arg1	ovaries					875:881	pig ovaries	871:881	pig ovaries	871:881	Bovine ovaries had more collagen than porcine ovaries when assessed biochemically, and this was associated with species-specific differences in collagen gene transcripts: Col3a1 was predominant in cow ovaries while Col1a1 was predominant in pig ovaries.
34440360	0	65	theme	Bovine	73:78	arg1	Ovaries					92:98	Bovine and Porcine Ovaries	73:98	Bovine and Porcine Ovaries	73:98	Hyaluronan and Collagen Are Prominent Extracellular Matrix Components in Bovine and Porcine Ovaries.
34440360	2	66	theme	ovarian	218:224	arg1	Collagen					176:183	Collagen	176:183	Collagen	176:183	Collagen and hyaluronan (HA) are critical ovarian stromal ECM molecules that undergo age-dependent changes in the mouse and human.
34440360	2	66	theme	ovarian	218:224	arg1	molecules					238:246	critical ovarian stromal ECM molecules	209:246	critical ovarian stromal ECM molecules that undergo age-dependent changes in the mouse and human	209:304	Collagen and hyaluronan (HA) are critical ovarian stromal ECM molecules that undergo age-dependent changes in the mouse and human.
34440360	2	66	theme	ovarian	218:224	arg1	hyaluronan					189:198	hyaluronan	189:198	hyaluronan (HA)	189:203	Collagen and hyaluronan (HA) are critical ovarian stromal ECM molecules that undergo age-dependent changes in the mouse and human.
34440360	5	67	theme	pig	871:873	arg1	ovaries					875:881	pig ovaries	871:881	pig ovaries	871:881	Bovine ovaries had more collagen than porcine ovaries when assessed biochemically, and this was associated with species-specific differences in collagen gene transcripts: Col3a1 was predominant in cow ovaries while Col1a1 was predominant in pig ovaries.
34440360	8	68	from	ovaries	1247:1253	arg1	predominant					1228:1238	predominant	1228:1238	predominant	1228:1238	Transcripts for HA synthesis and degradation enzymes, Has3 and Hyal2, respectively, were predominant in cow ovaries, whereas Has2, Kiaa1199, and Tmem2 tended to be predominant in pig ovaries.
34440360	8	69	attach	predominant	1303:1313	arg2	Tmem2					1284:1288	Tmem2	1284:1288	Tmem2	1284:1288	Transcripts for HA synthesis and degradation enzymes, Has3 and Hyal2, respectively, were predominant in cow ovaries, whereas Has2, Kiaa1199, and Tmem2 tended to be predominant in pig ovaries.
34440360	8	69	attach	predominant	1303:1313	arg2	Kiaa1199					1270:1277	Kiaa1199	1270:1277	Kiaa1199	1270:1277	Transcripts for HA synthesis and degradation enzymes, Has3 and Hyal2, respectively, were predominant in cow ovaries, whereas Has2, Kiaa1199, and Tmem2 tended to be predominant in pig ovaries.
34440360	8	69	attach	predominant	1303:1313	arg2	Has2					1264:1267	Has2	1264:1267	Has2	1264:1267	Transcripts for HA synthesis and degradation enzymes, Has3 and Hyal2, respectively, were predominant in cow ovaries, whereas Has2, Kiaa1199, and Tmem2 tended to be predominant in pig ovaries.
34440360	8	69	attach	predominant	1303:1313	arg1	ovaries					1322:1328	pig ovaries	1318:1328	pig ovaries	1318:1328	Transcripts for HA synthesis and degradation enzymes, Has3 and Hyal2, respectively, were predominant in cow ovaries, whereas Has2, Kiaa1199, and Tmem2 tended to be predominant in pig ovaries.
34440360	5	70	theme	gene	783:786	arg1	transcripts					788:798	collagen gene transcripts	774:798	collagen gene transcripts	774:798	Bovine ovaries had more collagen than porcine ovaries when assessed biochemically, and this was associated with species-specific differences in collagen gene transcripts: Col3a1 was predominant in cow ovaries while Col1a1 was predominant in pig ovaries.
34440360	2	71	theme	critical	209:216	arg1	Collagen					176:183	Collagen	176:183	Collagen	176:183	Collagen and hyaluronan (HA) are critical ovarian stromal ECM molecules that undergo age-dependent changes in the mouse and human.
34440360	2	71	theme	critical	209:216	arg1	molecules					238:246	critical ovarian stromal ECM molecules	209:246	critical ovarian stromal ECM molecules that undergo age-dependent changes in the mouse and human	209:304	Collagen and hyaluronan (HA) are critical ovarian stromal ECM molecules that undergo age-dependent changes in the mouse and human.
34440360	2	71	theme	critical	209:216	arg1	hyaluronan					189:198	hyaluronan	189:198	hyaluronan (HA)	189:203	Collagen and hyaluronan (HA) are critical ovarian stromal ECM molecules that undergo age-dependent changes in the mouse and human.
34440360	9	72	theme	ovarian	1429:1435	arg1	ECM					1437:1439	the ovarian ECM	1425:1439	the ovarian ECM	1425:1439	Together, our findings have implications for the composition, organization, and regulation of the ovarian ECM in large mammalian species, including humans.
31945053	7	0	theme	low	1469:1471	arg1	concentration					1473:1485	low concentration	1469:1485	low concentration	1469:1485	However, these traction forces do not linearly translate into increased motility due to a biphasic role of cell adhesions in cell migration: at low concentration Matrigel promotes migration-effective tractions exerted through a high number of small sized focal adhesions.
31945053	8	1	theme	larger	1685:1690	arg1	adhesions					1698:1706	fewer, but larger focal adhesions	1674:1706	fewer, but larger focal adhesions that favor attachment yielding lower cell motility	1674:1757	However, at high Matrigel concentration, traction forces are exerted through fewer, but larger focal adhesions that favor attachment yielding lower cell motility.
31945053	8	2	theme	traction	1638:1645	arg1	forces					1647:1652	traction forces	1638:1652	traction forces	1638:1652	However, at high Matrigel concentration, traction forces are exerted through fewer, but larger focal adhesions that favor attachment yielding lower cell motility.
31945053	5	3	from	activity	1155:1162	arg1	cells					1185:1189	H1299 lung cancer cells	1167:1189	H1299 lung cancer cells	1167:1189	We quantitatively show that the presence of Matrigel increases hydrogel stiffness, which promotes β1 integrin expression and metalloproteinase activity in H1299 lung cancer cells.
31945053	1	4	theme	cells	243:247	arg1	migration					223:231	The migration	219:231	The migration of cancer cells	219:247	The migration of cancer cells is highly regulated by the biomechanical properties of their local microenvironment.
31945053	7	5	theme	cell	1450:1453	arg1	migration					1455:1463	cell migration	1450:1463	cell migration	1450:1463	However, these traction forces do not linearly translate into increased motility due to a biphasic role of cell adhesions in cell migration: at low concentration Matrigel promotes migration-effective tractions exerted through a high number of small sized focal adhesions.
31945053	4	6	theme	cell	795:798	arg1	migration					800:808	cancer cell migration	788:808	cancer cell migration	788:808	Here, we present a comprehensive, quantitative analysis of the role of cell-ECM interactions in cancer cell migration within a highly physiological environment consisting of mixed Matrigel-collagen hydrogel scaffolds of increasing complexity that mimic the tumor microenvironment at the leading edge of cancer invasion.
31945053	2	7	theme	signal	423:428	arg1	transduction					430:441	signal transduction	423:441	signal transduction	423:441	Using 3D scaffolds of simple composition, several aspects of cancer cell mechanosensing (signal transduction, EMC remodeling, traction forces) have been separately analyzed in the context of cell migration.
31945053	2	7	theme	signal	423:428	arg1	remodeling					448:457	EMC remodeling	444:457	EMC remodeling	444:457	Using 3D scaffolds of simple composition, several aspects of cancer cell mechanosensing (signal transduction, EMC remodeling, traction forces) have been separately analyzed in the context of cell migration.
31945053	2	7	theme	signal	423:428	arg1	forces					469:474	traction forces	460:474	traction forces	460:474	Using 3D scaffolds of simple composition, several aspects of cancer cell mechanosensing (signal transduction, EMC remodeling, traction forces) have been separately analyzed in the context of cell migration.
31945053	7	8	theme	adhesions	1437:1445	arg1	role					1424:1427	a biphasic role	1413:1427	a biphasic role of cell adhesions in cell migration	1413:1463	However, these traction forces do not linearly translate into increased motility due to a biphasic role of cell adhesions in cell migration: at low concentration Matrigel promotes migration-effective tractions exerted through a high number of small sized focal adhesions.
31945053	7	9	theme	due	1406:1408	arg1	motility					1397:1404	increased motility	1387:1404	increased motility due to a biphasic role of cell adhesions in cell migration	1387:1463	However, these traction forces do not linearly translate into increased motility due to a biphasic role of cell adhesions in cell migration: at low concentration Matrigel promotes migration-effective tractions exerted through a high number of small sized focal adhesions.
31945053	2	10	theme	cell	525:528	arg1	migration					530:538	cell migration	525:538	cell migration	525:538	Using 3D scaffolds of simple composition, several aspects of cancer cell mechanosensing (signal transduction, EMC remodeling, traction forces) have been separately analyzed in the context of cell migration.
31945053	7	11	theme	increased	1387:1395	arg1	motility					1397:1404	increased motility	1387:1404	increased motility due to a biphasic role of cell adhesions in cell migration	1387:1463	However, these traction forces do not linearly translate into increased motility due to a biphasic role of cell adhesions in cell migration: at low concentration Matrigel promotes migration-effective tractions exerted through a high number of small sized focal adhesions.
31945053	8	12	theme	cell	1745:1748	arg1	motility					1750:1757	lower cell motility	1739:1757	lower cell motility	1739:1757	However, at high Matrigel concentration, traction forces are exerted through fewer, but larger focal adhesions that favor attachment yielding lower cell motility.
31945053	4	13	theme	increasing	912:921	arg1	complexity					923:932	increasing complexity	912:932	increasing complexity	912:932	Here, we present a comprehensive, quantitative analysis of the role of cell-ECM interactions in cancer cell migration within a highly physiological environment consisting of mixed Matrigel-collagen hydrogel scaffolds of increasing complexity that mimic the tumor microenvironment at the leading edge of cancer invasion.
31945053	0	14	theme	cell	128:131	arg1	migration					133:141	cancer cell migration	121:141	cancer cell migration	121:141	The use of mixed collagen-Matrigel matrices of increasing complexity recapitulates the biphasic role of cell adhesion in cancer cell migration: ECM sensing, remodeling and forces at the leading edge of cancer invasion.
31945053	6	15	theme	ECM	1211:1213	arg1	activity					1226:1233	ECM remodeling activity	1211:1233	ECM remodeling activity	1211:1233	Then, we show that ECM remodeling activity causes matrix alignment and compaction that favors higher tractions exerted by the cells.
31945053	3	16	theme	combined	552:559	arg1	study					561:565	a combined study	550:565	a combined study of these factors in 3D scaffolds that more closely resemble the complex microenvironment of the cancer ECM	550:672	However, a combined study of these factors in 3D scaffolds that more closely resemble the complex microenvironment of the cancer ECM is still missing.
31945053	4	17	theme	Matrigel-collagen	872:888	arg1	scaffolds					899:907	mixed Matrigel-collagen hydrogel scaffolds	866:907	mixed Matrigel-collagen hydrogel scaffolds of increasing complexity that mimic the tumor microenvironment at the leading edge of cancer invasion	866:1009	Here, we present a comprehensive, quantitative analysis of the role of cell-ECM interactions in cancer cell migration within a highly physiological environment consisting of mixed Matrigel-collagen hydrogel scaffolds of increasing complexity that mimic the tumor microenvironment at the leading edge of cancer invasion.
31945053	3	18	theme	3D	587:588	arg1	scaffolds					590:598	3D scaffolds	587:598	3D scaffolds that more closely resemble the complex microenvironment of the cancer ECM	587:672	However, a combined study of these factors in 3D scaffolds that more closely resemble the complex microenvironment of the cancer ECM is still missing.
31945053	4	19	from	role	755:758	arg1	migration					800:808	cancer cell migration	788:808	cancer cell migration	788:808	Here, we present a comprehensive, quantitative analysis of the role of cell-ECM interactions in cancer cell migration within a highly physiological environment consisting of mixed Matrigel-collagen hydrogel scaffolds of increasing complexity that mimic the tumor microenvironment at the leading edge of cancer invasion.
31945053	0	20	from	role	96:99	arg1	forces					172:177	forces	172:177	forces	172:177	The use of mixed collagen-Matrigel matrices of increasing complexity recapitulates the biphasic role of cell adhesion in cancer cell migration: ECM sensing, remodeling and forces at the leading edge of cancer invasion.
31945053	0	20	from	role	96:99	arg1	edge					194:197	the leading edge	182:197	the leading edge of cancer invasion	182:216	The use of mixed collagen-Matrigel matrices of increasing complexity recapitulates the biphasic role of cell adhesion in cancer cell migration: ECM sensing, remodeling and forces at the leading edge of cancer invasion.
31945053	0	20	from	role	96:99	arg1	remodeling					157:166	remodeling	157:166	remodeling	157:166	The use of mixed collagen-Matrigel matrices of increasing complexity recapitulates the biphasic role of cell adhesion in cancer cell migration: ECM sensing, remodeling and forces at the leading edge of cancer invasion.
31945053	0	20	from	role	96:99	arg1	migration					133:141	cancer cell migration	121:141	cancer cell migration	121:141	The use of mixed collagen-Matrigel matrices of increasing complexity recapitulates the biphasic role of cell adhesion in cancer cell migration: ECM sensing, remodeling and forces at the leading edge of cancer invasion.
31945053	0	20	from	role	96:99	arg1	sensing					148:154	ECM sensing	144:154	ECM sensing	144:154	The use of mixed collagen-Matrigel matrices of increasing complexity recapitulates the biphasic role of cell adhesion in cancer cell migration: ECM sensing, remodeling and forces at the leading edge of cancer invasion.
31945053	0	21	theme	complexity	58:67	arg1	use					4:6	The use	0:6	The use of mixed collagen-Matrigel matrices of increasing complexity	0:67	The use of mixed collagen-Matrigel matrices of increasing complexity recapitulates the biphasic role of cell adhesion in cancer cell migration: ECM sensing, remodeling and forces at the leading edge of cancer invasion.
31945053	5	22	theme	β1	1110:1111	arg1	expression					1122:1131	β1 integrin expression	1110:1131	β1 integrin expression	1110:1131	We quantitatively show that the presence of Matrigel increases hydrogel stiffness, which promotes β1 integrin expression and metalloproteinase activity in H1299 lung cancer cells.
31945053	3	23	theme	factors	576:582	arg1	study					561:565	a combined study	550:565	a combined study of these factors in 3D scaffolds that more closely resemble the complex microenvironment of the cancer ECM	550:672	However, a combined study of these factors in 3D scaffolds that more closely resemble the complex microenvironment of the cancer ECM is still missing.
31945053	7	24	theme	focal	1580:1584	arg1	adhesions					1586:1594	small sized focal adhesions	1568:1594	small sized focal adhesions	1568:1594	However, these traction forces do not linearly translate into increased motility due to a biphasic role of cell adhesions in cell migration: at low concentration Matrigel promotes migration-effective tractions exerted through a high number of small sized focal adhesions.
31945053	2	25	theme	migration	530:538	arg1	context					514:520	the context	510:520	the context of cell migration	510:538	Using 3D scaffolds of simple composition, several aspects of cancer cell mechanosensing (signal transduction, EMC remodeling, traction forces) have been separately analyzed in the context of cell migration.
31945053	1	26	theme	microenvironment	316:331	arg1	properties					290:299	the biomechanical properties	272:299	the biomechanical properties of their local microenvironment	272:331	The migration of cancer cells is highly regulated by the biomechanical properties of their local microenvironment.
31945053	0	27	theme	invasion	209:216	arg1	edge					194:197	the leading edge	182:197	the leading edge of cancer invasion	182:216	The use of mixed collagen-Matrigel matrices of increasing complexity recapitulates the biphasic role of cell adhesion in cancer cell migration: ECM sensing, remodeling and forces at the leading edge of cancer invasion.
31945053	7	28	from	role	1424:1427	arg1	migration					1455:1463	cell migration	1450:1463	cell migration	1450:1463	However, these traction forces do not linearly translate into increased motility due to a biphasic role of cell adhesions in cell migration: at low concentration Matrigel promotes migration-effective tractions exerted through a high number of small sized focal adhesions.
31945053	7	29	theme	small	1568:1572	arg1	adhesions					1586:1594	small sized focal adhesions	1568:1594	small sized focal adhesions	1568:1594	However, these traction forces do not linearly translate into increased motility due to a biphasic role of cell adhesions in cell migration: at low concentration Matrigel promotes migration-effective tractions exerted through a high number of small sized focal adhesions.
31945053	4	30	theme	comprehensive	711:723	arg1	analysis					739:746	a comprehensive, quantitative analysis	709:746	a comprehensive, quantitative analysis of the role of cell-ECM interactions in cancer cell migration	709:808	Here, we present a comprehensive, quantitative analysis of the role of cell-ECM interactions in cancer cell migration within a highly physiological environment consisting of mixed Matrigel-collagen hydrogel scaffolds of increasing complexity that mimic the tumor microenvironment at the leading edge of cancer invasion.
31945053	2	31	theme	several	376:382	arg1	aspects					384:390	several aspects	376:390	several aspects of cancer cell mechanosensing (signal transduction, EMC remodeling, traction forces)	376:475	Using 3D scaffolds of simple composition, several aspects of cancer cell mechanosensing (signal transduction, EMC remodeling, traction forces) have been separately analyzed in the context of cell migration.
31945053	0	32	theme	cell	104:107	arg1	adhesion					109:116	cell adhesion	104:116	cell adhesion	104:116	The use of mixed collagen-Matrigel matrices of increasing complexity recapitulates the biphasic role of cell adhesion in cancer cell migration: ECM sensing, remodeling and forces at the leading edge of cancer invasion.
31945053	2	33	dep	mechanosensing	407:420	arg1	transduction					430:441	signal transduction	423:441	signal transduction	423:441	Using 3D scaffolds of simple composition, several aspects of cancer cell mechanosensing (signal transduction, EMC remodeling, traction forces) have been separately analyzed in the context of cell migration.
31945053	2	33	dep	mechanosensing	407:420	arg1	remodeling					448:457	EMC remodeling	444:457	EMC remodeling	444:457	Using 3D scaffolds of simple composition, several aspects of cancer cell mechanosensing (signal transduction, EMC remodeling, traction forces) have been separately analyzed in the context of cell migration.
31945053	2	33	dep	mechanosensing	407:420	arg1	forces					469:474	traction forces	460:474	traction forces	460:474	Using 3D scaffolds of simple composition, several aspects of cancer cell mechanosensing (signal transduction, EMC remodeling, traction forces) have been separately analyzed in the context of cell migration.
31945053	2	34	theme	composition	363:373	arg1	scaffolds					343:351	3D scaffolds	340:351	3D scaffolds of simple composition	340:373	Using 3D scaffolds of simple composition, several aspects of cancer cell mechanosensing (signal transduction, EMC remodeling, traction forces) have been separately analyzed in the context of cell migration.
31945053	5	35	theme	lung	1173:1176	arg1	cells					1185:1189	H1299 lung cancer cells	1167:1189	H1299 lung cancer cells	1167:1189	We quantitatively show that the presence of Matrigel increases hydrogel stiffness, which promotes β1 integrin expression and metalloproteinase activity in H1299 lung cancer cells.
31945053	2	36	theme	cell	402:405	arg1	mechanosensing					407:420	cancer cell mechanosensing	395:420	cancer cell mechanosensing (signal transduction, EMC remodeling, traction forces)	395:475	Using 3D scaffolds of simple composition, several aspects of cancer cell mechanosensing (signal transduction, EMC remodeling, traction forces) have been separately analyzed in the context of cell migration.
31945053	4	37	theme	cell-ECM	763:770	arg1	interactions					772:783	cell-ECM interactions	763:783	cell-ECM interactions	763:783	Here, we present a comprehensive, quantitative analysis of the role of cell-ECM interactions in cancer cell migration within a highly physiological environment consisting of mixed Matrigel-collagen hydrogel scaffolds of increasing complexity that mimic the tumor microenvironment at the leading edge of cancer invasion.
31945053	0	38	theme	collagen-Matrigel	17:33	arg1	matrices					35:42	mixed collagen-Matrigel matrices	11:42	mixed collagen-Matrigel matrices	11:42	The use of mixed collagen-Matrigel matrices of increasing complexity recapitulates the biphasic role of cell adhesion in cancer cell migration: ECM sensing, remodeling and forces at the leading edge of cancer invasion.
31945053	5	39	theme	metalloproteinase	1137:1153	arg1	activity					1155:1162	metalloproteinase activity	1137:1162	metalloproteinase activity	1137:1162	We quantitatively show that the presence of Matrigel increases hydrogel stiffness, which promotes β1 integrin expression and metalloproteinase activity in H1299 lung cancer cells.
31945053	4	40	theme	cancer	995:1000	arg1	invasion					1002:1009	cancer invasion	995:1009	cancer invasion	995:1009	Here, we present a comprehensive, quantitative analysis of the role of cell-ECM interactions in cancer cell migration within a highly physiological environment consisting of mixed Matrigel-collagen hydrogel scaffolds of increasing complexity that mimic the tumor microenvironment at the leading edge of cancer invasion.
31945053	0	41	from	edge	194:197	arg1	forces					172:177	forces	172:177	forces	172:177	The use of mixed collagen-Matrigel matrices of increasing complexity recapitulates the biphasic role of cell adhesion in cancer cell migration: ECM sensing, remodeling and forces at the leading edge of cancer invasion.
31945053	0	41	from	edge	194:197	arg1	remodeling					157:166	remodeling	157:166	remodeling	157:166	The use of mixed collagen-Matrigel matrices of increasing complexity recapitulates the biphasic role of cell adhesion in cancer cell migration: ECM sensing, remodeling and forces at the leading edge of cancer invasion.
31945053	0	41	from	edge	194:197	arg1	migration					133:141	cancer cell migration	121:141	cancer cell migration	121:141	The use of mixed collagen-Matrigel matrices of increasing complexity recapitulates the biphasic role of cell adhesion in cancer cell migration: ECM sensing, remodeling and forces at the leading edge of cancer invasion.
31945053	0	41	from	edge	194:197	arg1	sensing					148:154	ECM sensing	144:154	ECM sensing	144:154	The use of mixed collagen-Matrigel matrices of increasing complexity recapitulates the biphasic role of cell adhesion in cancer cell migration: ECM sensing, remodeling and forces at the leading edge of cancer invasion.
31945053	0	41	from	edge	194:197	arg1	role					96:99	the biphasic role	83:99	the biphasic role of cell adhesion in cancer cell migration: ECM sensing, remodeling and forces at the leading edge of cancer invasion	83:216	The use of mixed collagen-Matrigel matrices of increasing complexity recapitulates the biphasic role of cell adhesion in cancer cell migration: ECM sensing, remodeling and forces at the leading edge of cancer invasion.
31945053	4	42	theme	role	755:758	arg1	analysis					739:746	a comprehensive, quantitative analysis	709:746	a comprehensive, quantitative analysis of the role of cell-ECM interactions in cancer cell migration	709:808	Here, we present a comprehensive, quantitative analysis of the role of cell-ECM interactions in cancer cell migration within a highly physiological environment consisting of mixed Matrigel-collagen hydrogel scaffolds of increasing complexity that mimic the tumor microenvironment at the leading edge of cancer invasion.
31945053	2	43	theme	EMC	444:446	arg1	transduction					430:441	signal transduction	423:441	signal transduction	423:441	Using 3D scaffolds of simple composition, several aspects of cancer cell mechanosensing (signal transduction, EMC remodeling, traction forces) have been separately analyzed in the context of cell migration.
31945053	2	43	theme	EMC	444:446	arg1	remodeling					448:457	EMC remodeling	444:457	EMC remodeling	444:457	Using 3D scaffolds of simple composition, several aspects of cancer cell mechanosensing (signal transduction, EMC remodeling, traction forces) have been separately analyzed in the context of cell migration.
31945053	3	44	theme	ECM	670:672	arg1	microenvironment					639:654	the complex microenvironment	627:654	the complex microenvironment of the cancer ECM	627:672	However, a combined study of these factors in 3D scaffolds that more closely resemble the complex microenvironment of the cancer ECM is still missing.
31945053	6	45	theme	higher	1286:1291	arg1	tractions					1293:1301	higher tractions	1286:1301	higher tractions exerted by the cells	1286:1322	Then, we show that ECM remodeling activity causes matrix alignment and compaction that favors higher tractions exerted by the cells.
31945053	2	46	theme	3D	340:341	arg1	scaffolds					343:351	3D scaffolds	340:351	3D scaffolds of simple composition	340:373	Using 3D scaffolds of simple composition, several aspects of cancer cell mechanosensing (signal transduction, EMC remodeling, traction forces) have been separately analyzed in the context of cell migration.
31945053	8	47	theme	fewer	1674:1678	arg1	adhesions					1698:1706	fewer, but larger focal adhesions	1674:1706	fewer, but larger focal adhesions that favor attachment yielding lower cell motility	1674:1757	However, at high Matrigel concentration, traction forces are exerted through fewer, but larger focal adhesions that favor attachment yielding lower cell motility.
31945053	5	48	theme	integrin	1113:1120	arg1	expression					1122:1131	β1 integrin expression	1110:1131	β1 integrin expression	1110:1131	We quantitatively show that the presence of Matrigel increases hydrogel stiffness, which promotes β1 integrin expression and metalloproteinase activity in H1299 lung cancer cells.
31945053	8	49	theme	Matrigel	1614:1621	arg1	concentration					1623:1635	high Matrigel concentration	1609:1635	high Matrigel concentration	1609:1635	However, at high Matrigel concentration, traction forces are exerted through fewer, but larger focal adhesions that favor attachment yielding lower cell motility.
31945053	1	50	theme	cancer	236:241	arg1	cells					243:247	cancer cells	236:247	cancer cells	236:247	The migration of cancer cells is highly regulated by the biomechanical properties of their local microenvironment.
31945053	4	51	theme	physiological	826:838	arg1	environment					840:850	a highly physiological environment	817:850	a highly physiological environment consisting of mixed Matrigel-collagen hydrogel scaffolds of increasing complexity that mimic the tumor microenvironment at the leading edge of cancer invasion	817:1009	Here, we present a comprehensive, quantitative analysis of the role of cell-ECM interactions in cancer cell migration within a highly physiological environment consisting of mixed Matrigel-collagen hydrogel scaffolds of increasing complexity that mimic the tumor microenvironment at the leading edge of cancer invasion.
31945053	0	52	theme	leading	186:192	arg1	edge					194:197	the leading edge	182:197	the leading edge of cancer invasion	182:216	The use of mixed collagen-Matrigel matrices of increasing complexity recapitulates the biphasic role of cell adhesion in cancer cell migration: ECM sensing, remodeling and forces at the leading edge of cancer invasion.
31945053	8	53	theme	focal	1692:1696	arg1	adhesions					1698:1706	fewer, but larger focal adhesions	1674:1706	fewer, but larger focal adhesions that favor attachment yielding lower cell motility	1674:1757	However, at high Matrigel concentration, traction forces are exerted through fewer, but larger focal adhesions that favor attachment yielding lower cell motility.
31945053	7	54	theme	cell	1432:1435	arg1	adhesions					1437:1445	cell adhesions	1432:1445	cell adhesions	1432:1445	However, these traction forces do not linearly translate into increased motility due to a biphasic role of cell adhesions in cell migration: at low concentration Matrigel promotes migration-effective tractions exerted through a high number of small sized focal adhesions.
31945053	4	55	theme	cancer	788:793	arg1	migration					800:808	cancer cell migration	788:808	cancer cell migration	788:808	Here, we present a comprehensive, quantitative analysis of the role of cell-ECM interactions in cancer cell migration within a highly physiological environment consisting of mixed Matrigel-collagen hydrogel scaffolds of increasing complexity that mimic the tumor microenvironment at the leading edge of cancer invasion.
31945053	7	56	theme	biphasic	1415:1422	arg1	role					1424:1427	a biphasic role	1413:1427	a biphasic role of cell adhesions in cell migration	1413:1463	However, these traction forces do not linearly translate into increased motility due to a biphasic role of cell adhesions in cell migration: at low concentration Matrigel promotes migration-effective tractions exerted through a high number of small sized focal adhesions.
31945053	6	57	theme	matrix	1242:1247	arg1	alignment					1249:1257	matrix alignment	1242:1257	matrix alignment	1242:1257	Then, we show that ECM remodeling activity causes matrix alignment and compaction that favors higher tractions exerted by the cells.
31945053	0	58	theme	cancer	121:126	arg1	migration					133:141	cancer cell migration	121:141	cancer cell migration	121:141	The use of mixed collagen-Matrigel matrices of increasing complexity recapitulates the biphasic role of cell adhesion in cancer cell migration: ECM sensing, remodeling and forces at the leading edge of cancer invasion.
31945053	4	59	theme	hydrogel	890:897	arg1	scaffolds					899:907	mixed Matrigel-collagen hydrogel scaffolds	866:907	mixed Matrigel-collagen hydrogel scaffolds of increasing complexity that mimic the tumor microenvironment at the leading edge of cancer invasion	866:1009	Here, we present a comprehensive, quantitative analysis of the role of cell-ECM interactions in cancer cell migration within a highly physiological environment consisting of mixed Matrigel-collagen hydrogel scaffolds of increasing complexity that mimic the tumor microenvironment at the leading edge of cancer invasion.
31945053	5	60	theme	Matrigel	1056:1063	arg1	presence					1044:1051	the presence	1040:1051	the presence of Matrigel	1040:1063	We quantitatively show that the presence of Matrigel increases hydrogel stiffness, which promotes β1 integrin expression and metalloproteinase activity in H1299 lung cancer cells.
31945053	8	61	theme	lower	1739:1743	arg1	motility					1750:1757	lower cell motility	1739:1757	lower cell motility	1739:1757	However, at high Matrigel concentration, traction forces are exerted through fewer, but larger focal adhesions that favor attachment yielding lower cell motility.
31945053	6	62	theme	remodeling	1215:1224	arg1	activity					1226:1233	ECM remodeling activity	1211:1233	ECM remodeling activity	1211:1233	Then, we show that ECM remodeling activity causes matrix alignment and compaction that favors higher tractions exerted by the cells.
31945053	0	63	theme	ECM	144:146	arg1	sensing					148:154	ECM sensing	144:154	ECM sensing	144:154	The use of mixed collagen-Matrigel matrices of increasing complexity recapitulates the biphasic role of cell adhesion in cancer cell migration: ECM sensing, remodeling and forces at the leading edge of cancer invasion.
31945053	5	64	theme	hydrogel	1075:1082	arg1	stiffness					1084:1092	hydrogel stiffness	1075:1092	hydrogel stiffness	1075:1092	We quantitatively show that the presence of Matrigel increases hydrogel stiffness, which promotes β1 integrin expression and metalloproteinase activity in H1299 lung cancer cells.
31945053	0	65	theme	matrices	35:42	arg1	use					4:6	The use	0:6	The use of mixed collagen-Matrigel matrices of increasing complexity	0:67	The use of mixed collagen-Matrigel matrices of increasing complexity recapitulates the biphasic role of cell adhesion in cancer cell migration: ECM sensing, remodeling and forces at the leading edge of cancer invasion.
31945053	7	66	theme	adhesions	1586:1594	arg1	number					1558:1563	a high number	1551:1563	a high number of small sized focal adhesions	1551:1594	However, these traction forces do not linearly translate into increased motility due to a biphasic role of cell adhesions in cell migration: at low concentration Matrigel promotes migration-effective tractions exerted through a high number of small sized focal adhesions.
31945053	4	67	theme	mixed	866:870	arg1	scaffolds					899:907	mixed Matrigel-collagen hydrogel scaffolds	866:907	mixed Matrigel-collagen hydrogel scaffolds of increasing complexity that mimic the tumor microenvironment at the leading edge of cancer invasion	866:1009	Here, we present a comprehensive, quantitative analysis of the role of cell-ECM interactions in cancer cell migration within a highly physiological environment consisting of mixed Matrigel-collagen hydrogel scaffolds of increasing complexity that mimic the tumor microenvironment at the leading edge of cancer invasion.
31945053	7	68	theme	sized	1574:1578	arg1	adhesions					1586:1594	small sized focal adhesions	1568:1594	small sized focal adhesions	1568:1594	However, these traction forces do not linearly translate into increased motility due to a biphasic role of cell adhesions in cell migration: at low concentration Matrigel promotes migration-effective tractions exerted through a high number of small sized focal adhesions.
31945053	4	69	theme	tumor	949:953	arg1	microenvironment					955:970	the tumor microenvironment	945:970	the tumor microenvironment	945:970	Here, we present a comprehensive, quantitative analysis of the role of cell-ECM interactions in cancer cell migration within a highly physiological environment consisting of mixed Matrigel-collagen hydrogel scaffolds of increasing complexity that mimic the tumor microenvironment at the leading edge of cancer invasion.
31945053	7	70	theme	traction	1340:1347	arg1	forces					1349:1354	these traction forces	1334:1354	these traction forces	1334:1354	However, these traction forces do not linearly translate into increased motility due to a biphasic role of cell adhesions in cell migration: at low concentration Matrigel promotes migration-effective tractions exerted through a high number of small sized focal adhesions.
31945053	0	71	theme	biphasic	87:94	arg1	role					96:99	the biphasic role	83:99	the biphasic role of cell adhesion in cancer cell migration: ECM sensing, remodeling and forces at the leading edge of cancer invasion	83:216	The use of mixed collagen-Matrigel matrices of increasing complexity recapitulates the biphasic role of cell adhesion in cancer cell migration: ECM sensing, remodeling and forces at the leading edge of cancer invasion.
31945053	1	72	theme	local	310:314	arg1	microenvironment					316:331	their local microenvironment	304:331	their local microenvironment	304:331	The migration of cancer cells is highly regulated by the biomechanical properties of their local microenvironment.
31945053	4	73	from	analysis	739:746	arg1	migration					800:808	cancer cell migration	788:808	cancer cell migration	788:808	Here, we present a comprehensive, quantitative analysis of the role of cell-ECM interactions in cancer cell migration within a highly physiological environment consisting of mixed Matrigel-collagen hydrogel scaffolds of increasing complexity that mimic the tumor microenvironment at the leading edge of cancer invasion.
31945053	4	74	dep	comprehensive	711:723	arg1	quantitative					726:737	quantitative	726:737	quantitative	726:737	Here, we present a comprehensive, quantitative analysis of the role of cell-ECM interactions in cancer cell migration within a highly physiological environment consisting of mixed Matrigel-collagen hydrogel scaffolds of increasing complexity that mimic the tumor microenvironment at the leading edge of cancer invasion.
31945053	2	75	theme	traction	460:467	arg1	transduction					430:441	signal transduction	423:441	signal transduction	423:441	Using 3D scaffolds of simple composition, several aspects of cancer cell mechanosensing (signal transduction, EMC remodeling, traction forces) have been separately analyzed in the context of cell migration.
31945053	2	75	theme	traction	460:467	arg1	forces					469:474	traction forces	460:474	traction forces	460:474	Using 3D scaffolds of simple composition, several aspects of cancer cell mechanosensing (signal transduction, EMC remodeling, traction forces) have been separately analyzed in the context of cell migration.
31945053	0	76	theme	cancer	202:207	arg1	invasion					209:216	cancer invasion	202:216	cancer invasion	202:216	The use of mixed collagen-Matrigel matrices of increasing complexity recapitulates the biphasic role of cell adhesion in cancer cell migration: ECM sensing, remodeling and forces at the leading edge of cancer invasion.
31945053	7	77	theme	high	1553:1556	arg1	number					1558:1563	a high number	1551:1563	a high number of small sized focal adhesions	1551:1594	However, these traction forces do not linearly translate into increased motility due to a biphasic role of cell adhesions in cell migration: at low concentration Matrigel promotes migration-effective tractions exerted through a high number of small sized focal adhesions.
31945053	4	78	theme	complexity	923:932	arg1	scaffolds					899:907	mixed Matrigel-collagen hydrogel scaffolds	866:907	mixed Matrigel-collagen hydrogel scaffolds of increasing complexity that mimic the tumor microenvironment at the leading edge of cancer invasion	866:1009	Here, we present a comprehensive, quantitative analysis of the role of cell-ECM interactions in cancer cell migration within a highly physiological environment consisting of mixed Matrigel-collagen hydrogel scaffolds of increasing complexity that mimic the tumor microenvironment at the leading edge of cancer invasion.
31945053	0	79	theme	adhesion	109:116	arg1	role					96:99	the biphasic role	83:99	the biphasic role of cell adhesion in cancer cell migration: ECM sensing, remodeling and forces at the leading edge of cancer invasion	83:216	The use of mixed collagen-Matrigel matrices of increasing complexity recapitulates the biphasic role of cell adhesion in cancer cell migration: ECM sensing, remodeling and forces at the leading edge of cancer invasion.
31945053	4	80	from	migration	800:808	arg1	analysis					739:746	a comprehensive, quantitative analysis	709:746	a comprehensive, quantitative analysis of the role of cell-ECM interactions in cancer cell migration	709:808	Here, we present a comprehensive, quantitative analysis of the role of cell-ECM interactions in cancer cell migration within a highly physiological environment consisting of mixed Matrigel-collagen hydrogel scaffolds of increasing complexity that mimic the tumor microenvironment at the leading edge of cancer invasion.
31945053	4	81	theme	invasion	1002:1009	arg1	edge					987:990	the leading edge	975:990	the leading edge of cancer invasion	975:1009	Here, we present a comprehensive, quantitative analysis of the role of cell-ECM interactions in cancer cell migration within a highly physiological environment consisting of mixed Matrigel-collagen hydrogel scaffolds of increasing complexity that mimic the tumor microenvironment at the leading edge of cancer invasion.
31945053	2	82	theme	simple	356:361	arg1	composition					363:373	simple composition	356:373	simple composition	356:373	Using 3D scaffolds of simple composition, several aspects of cancer cell mechanosensing (signal transduction, EMC remodeling, traction forces) have been separately analyzed in the context of cell migration.
31945053	0	83	theme	mixed	11:15	arg1	matrices					35:42	mixed collagen-Matrigel matrices	11:42	mixed collagen-Matrigel matrices	11:42	The use of mixed collagen-Matrigel matrices of increasing complexity recapitulates the biphasic role of cell adhesion in cancer cell migration: ECM sensing, remodeling and forces at the leading edge of cancer invasion.
31945053	5	84	theme	cancer	1178:1183	arg1	cells					1185:1189	H1299 lung cancer cells	1167:1189	H1299 lung cancer cells	1167:1189	We quantitatively show that the presence of Matrigel increases hydrogel stiffness, which promotes β1 integrin expression and metalloproteinase activity in H1299 lung cancer cells.
31945053	2	85	theme	mechanosensing	407:420	arg1	aspects					384:390	several aspects	376:390	several aspects of cancer cell mechanosensing (signal transduction, EMC remodeling, traction forces)	376:475	Using 3D scaffolds of simple composition, several aspects of cancer cell mechanosensing (signal transduction, EMC remodeling, traction forces) have been separately analyzed in the context of cell migration.
31945053	3	86	from	study	561:565	arg1	scaffolds					590:598	3D scaffolds	587:598	3D scaffolds that more closely resemble the complex microenvironment of the cancer ECM	587:672	However, a combined study of these factors in 3D scaffolds that more closely resemble the complex microenvironment of the cancer ECM is still missing.
31945053	2	87	theme	cancer	395:400	arg1	mechanosensing					407:420	cancer cell mechanosensing	395:420	cancer cell mechanosensing (signal transduction, EMC remodeling, traction forces)	395:475	Using 3D scaffolds of simple composition, several aspects of cancer cell mechanosensing (signal transduction, EMC remodeling, traction forces) have been separately analyzed in the context of cell migration.
31945053	5	88	from	expression	1122:1131	arg1	cells					1185:1189	H1299 lung cancer cells	1167:1189	H1299 lung cancer cells	1167:1189	We quantitatively show that the presence of Matrigel increases hydrogel stiffness, which promotes β1 integrin expression and metalloproteinase activity in H1299 lung cancer cells.
31945053	4	89	theme	leading	979:985	arg1	edge					987:990	the leading edge	975:990	the leading edge of cancer invasion	975:1009	Here, we present a comprehensive, quantitative analysis of the role of cell-ECM interactions in cancer cell migration within a highly physiological environment consisting of mixed Matrigel-collagen hydrogel scaffolds of increasing complexity that mimic the tumor microenvironment at the leading edge of cancer invasion.
31945053	0	90	theme	increasing	47:56	arg1	complexity					58:67	increasing complexity	47:67	increasing complexity	47:67	The use of mixed collagen-Matrigel matrices of increasing complexity recapitulates the biphasic role of cell adhesion in cancer cell migration: ECM sensing, remodeling and forces at the leading edge of cancer invasion.
31945053	1	91	theme	biomechanical	276:288	arg1	properties					290:299	the biomechanical properties	272:299	the biomechanical properties of their local microenvironment	272:331	The migration of cancer cells is highly regulated by the biomechanical properties of their local microenvironment.
31945053	3	92	theme	complex	631:637	arg1	microenvironment					639:654	the complex microenvironment	627:654	the complex microenvironment of the cancer ECM	627:672	However, a combined study of these factors in 3D scaffolds that more closely resemble the complex microenvironment of the cancer ECM is still missing.
31945053	4	93	theme	interactions	772:783	arg1	role					755:758	the role	751:758	the role of cell-ECM interactions in cancer cell migration	751:808	Here, we present a comprehensive, quantitative analysis of the role of cell-ECM interactions in cancer cell migration within a highly physiological environment consisting of mixed Matrigel-collagen hydrogel scaffolds of increasing complexity that mimic the tumor microenvironment at the leading edge of cancer invasion.
31945053	5	94	theme	H1299	1167:1171	arg1	cells					1185:1189	H1299 lung cancer cells	1167:1189	H1299 lung cancer cells	1167:1189	We quantitatively show that the presence of Matrigel increases hydrogel stiffness, which promotes β1 integrin expression and metalloproteinase activity in H1299 lung cancer cells.
31945053	7	95	theme	migration-effective	1505:1523	arg1	tractions					1525:1533	migration-effective tractions	1505:1533	migration-effective tractions exerted through a high number of small sized focal adhesions	1505:1594	However, these traction forces do not linearly translate into increased motility due to a biphasic role of cell adhesions in cell migration: at low concentration Matrigel promotes migration-effective tractions exerted through a high number of small sized focal adhesions.
31945053	8	96	theme	high	1609:1612	arg1	concentration					1623:1635	high Matrigel concentration	1609:1635	high Matrigel concentration	1609:1635	However, at high Matrigel concentration, traction forces are exerted through fewer, but larger focal adhesions that favor attachment yielding lower cell motility.
31945053	3	97	theme	cancer	663:668	arg1	ECM					670:672	the cancer ECM	659:672	the cancer ECM	659:672	However, a combined study of these factors in 3D scaffolds that more closely resemble the complex microenvironment of the cancer ECM is still missing.
34095846	3	0	theme	synthetic	304:312	arg1	carbohydrates					314:326	synthetic carbohydrates	304:326	synthetic carbohydrates that mimic the structure of cell surface glycans	304:375	This process can be blocked by synthetic carbohydrates that mimic the structure of cell surface glycans.
34095846	5	1	attach	attached	833:840	arg3	μM					869:870	138 μM	865:870	138 μM	865:870	Galactosides 1 and 6, built on an aromatic core, were most efficient inhibitors of adhesion of C. albicans to buccal epithelial cells, displacing up to 36% and 48%, respectively, of yeast already attached to epithelial cells at 138 μM.
34095846	5	1	attach	attached	833:840	arg1	cells					856:860	epithelial cells	845:860	epithelial cells	845:860	Galactosides 1 and 6, built on an aromatic core, were most efficient inhibitors of adhesion of C. albicans to buccal epithelial cells, displacing up to 36% and 48%, respectively, of yeast already attached to epithelial cells at 138 μM.
34095846	5	1	attach	attached	833:840	arg2	yeast					819:823	yeast	819:823	yeast already attached to epithelial cells at 138 μM	819:870	Galactosides 1 and 6, built on an aromatic core, were most efficient inhibitors of adhesion of C. albicans to buccal epithelial cells, displacing up to 36% and 48%, respectively, of yeast already attached to epithelial cells at 138 μM.
34095846	5	2	theme	yeast	819:823	arg1	%					791:791	up to 36%	783:791	up to 36%	783:791	Galactosides 1 and 6, built on an aromatic core, were most efficient inhibitors of adhesion of C. albicans to buccal epithelial cells, displacing up to 36% and 48%, respectively, of yeast already attached to epithelial cells at 138 μM.
34095846	5	2	theme	yeast	819:823	arg1	%					799:799	48%	797:799	48%	797:799	Galactosides 1 and 6, built on an aromatic core, were most efficient inhibitors of adhesion of C. albicans to buccal epithelial cells, displacing up to 36% and 48%, respectively, of yeast already attached to epithelial cells at 138 μM.
34095846	5	2	theme	yeast	819:823	arg1	yeast					819:823	yeast	819:823	yeast already attached to epithelial cells at 138 μM	819:870	Galactosides 1 and 6, built on an aromatic core, were most efficient inhibitors of adhesion of C. albicans to buccal epithelial cells, displacing up to 36% and 48%, respectively, of yeast already attached to epithelial cells at 138 μM.
34095846	4	3	dep	aromatic	455:462	arg1	squaramide					474:483	squaramide	474:483	squaramide	474:483	Herein we report the evaluation of a series of divalent glycosides featuring aromatic (benzene, squaramide) and bicyclic aliphatic (norbornene) scaffolds, with the latter being the first examples of their kind as small molecule anti-adhesion glycoconjugates.
34095846	4	3	dep	aromatic	455:462	arg1	benzene					465:471	benzene	465:471	benzene	465:471	Herein we report the evaluation of a series of divalent glycosides featuring aromatic (benzene, squaramide) and bicyclic aliphatic (norbornene) scaffolds, with the latter being the first examples of their kind as small molecule anti-adhesion glycoconjugates.
34095846	4	3	dep	aromatic	455:462	arg1	aliphatic					499:507	aliphatic	499:507	aliphatic	499:507	Herein we report the evaluation of a series of divalent glycosides featuring aromatic (benzene, squaramide) and bicyclic aliphatic (norbornene) scaffolds, with the latter being the first examples of their kind as small molecule anti-adhesion glycoconjugates.
34095846	0	4	theme	adhesion	78:85	arg1	inhibitors					57:66	glycosylated inhibitors	44:66	glycosylated inhibitors of fungal adhesion	44:85	Scaffold diversity for enhanced activity of glycosylated inhibitors of fungal adhesion.
34095846	8	5	theme	new	1115:1117	arg1	potential					1085:1093	the potential	1081:1093	the potential of norbornenes	1081:1108	These results highlight the potential of norbornenes as a new class of aliphatic scaffolds for the synthesis of anti-adhesion compounds.
34095846	8	5	theme	new	1115:1117	arg1	class					1119:1123	a new class	1113:1123	a new class of aliphatic scaffolds for the synthesis of anti-adhesion compounds	1113:1191	These results highlight the potential of norbornenes as a new class of aliphatic scaffolds for the synthesis of anti-adhesion compounds.
34095846	4	6	theme	bicyclic	490:497	arg1	scaffolds					522:530	aromatic (benzene, squaramide) and bicyclic aliphatic (norbornene) scaffolds	455:530	aromatic (benzene, squaramide) and bicyclic aliphatic (norbornene) scaffolds	455:530	Herein we report the evaluation of a series of divalent glycosides featuring aromatic (benzene, squaramide) and bicyclic aliphatic (norbornene) scaffolds, with the latter being the first examples of their kind as small molecule anti-adhesion glycoconjugates.
34095846	4	6	theme	bicyclic	490:497	arg1	norbornene					510:519	norbornene	510:519	norbornene	510:519	Herein we report the evaluation of a series of divalent glycosides featuring aromatic (benzene, squaramide) and bicyclic aliphatic (norbornene) scaffolds, with the latter being the first examples of their kind as small molecule anti-adhesion glycoconjugates.
34095846	3	7	theme	cell	356:359	arg1	glycans					369:375	cell surface glycans	356:375	cell surface glycans	356:375	This process can be blocked by synthetic carbohydrates that mimic the structure of cell surface glycans.
34095846	4	8	theme	first	559:563	arg1	examples					565:572	the first examples	555:572	the first examples of their kind	555:586	Herein we report the evaluation of a series of divalent glycosides featuring aromatic (benzene, squaramide) and bicyclic aliphatic (norbornene) scaffolds, with the latter being the first examples of their kind as small molecule anti-adhesion glycoconjugates.
34095846	4	8	theme	first	559:563	arg1	latter					542:547	latter	542:547	latter	542:547	Herein we report the evaluation of a series of divalent glycosides featuring aromatic (benzene, squaramide) and bicyclic aliphatic (norbornene) scaffolds, with the latter being the first examples of their kind as small molecule anti-adhesion glycoconjugates.
34095846	5	9	theme	efficient	696:704	arg1	inhibitors					706:715	most efficient inhibitors	691:715	most efficient inhibitors of adhesion of C. albicans to buccal epithelial cells	691:769	Galactosides 1 and 6, built on an aromatic core, were most efficient inhibitors of adhesion of C. albicans to buccal epithelial cells, displacing up to 36% and 48%, respectively, of yeast already attached to epithelial cells at 138 μM.
34095846	7	10	theme	folded	1035:1040	arg1	conformations					1042:1054	folded conformations	1035:1054	folded conformations	1035:1054	Conformational analysis reveals a preference for compounds 1 and 21 to adopt folded conformations.
34095846	8	11	theme	scaffolds	1138:1146	arg1	potential					1085:1093	the potential	1081:1093	the potential of norbornenes	1081:1108	These results highlight the potential of norbornenes as a new class of aliphatic scaffolds for the synthesis of anti-adhesion compounds.
34095846	8	11	theme	scaffolds	1138:1146	arg1	class					1119:1123	a new class	1113:1123	a new class of aliphatic scaffolds for the synthesis of anti-adhesion compounds	1113:1191	These results highlight the potential of norbornenes as a new class of aliphatic scaffolds for the synthesis of anti-adhesion compounds.
34095846	1	12	theme	hospital	163:170	arg1	infections					181:190	hospital acquired infections	163:190	hospital acquired infections	163:190	Candida albicans is one of the most prevalent fungal pathogens involved in hospital acquired infections.
34095846	5	13	theme	epithelial	754:763	arg1	cells					765:769	buccal epithelial cells	747:769	buccal epithelial cells	747:769	Galactosides 1 and 6, built on an aromatic core, were most efficient inhibitors of adhesion of C. albicans to buccal epithelial cells, displacing up to 36% and 48%, respectively, of yeast already attached to epithelial cells at 138 μM.
34095846	6	14	theme	benzene-core	932:943	arg1	derivatives					945:955	benzene-core derivatives	932:955	benzene-core derivatives	932:955	Remarkably, cis-endo-norbornene 21 performed comparably to benzene-core derivatives.
34095846	1	15	theme	acquired	172:179	arg1	infections					181:190	hospital acquired infections	163:190	hospital acquired infections	163:190	Candida albicans is one of the most prevalent fungal pathogens involved in hospital acquired infections.
34095846	8	16	theme	aliphatic	1128:1136	arg1	scaffolds					1138:1146	aliphatic scaffolds	1128:1146	aliphatic scaffolds for the synthesis of anti-adhesion compounds	1128:1191	These results highlight the potential of norbornenes as a new class of aliphatic scaffolds for the synthesis of anti-adhesion compounds.
34095846	1	17	theme	Candida	88:94	arg1	albicans					96:103	Candida albicans	88:103	Candida albicans	88:103	Candida albicans is one of the most prevalent fungal pathogens involved in hospital acquired infections.
34095846	8	18	theme	norbornenes	1098:1108	arg1	potential					1085:1093	the potential	1081:1093	the potential of norbornenes	1081:1108	These results highlight the potential of norbornenes as a new class of aliphatic scaffolds for the synthesis of anti-adhesion compounds.
34095846	8	18	theme	norbornenes	1098:1108	arg1	class					1119:1123	a new class	1113:1123	a new class of aliphatic scaffolds for the synthesis of anti-adhesion compounds	1113:1191	These results highlight the potential of norbornenes as a new class of aliphatic scaffolds for the synthesis of anti-adhesion compounds.
34095846	3	19	theme	surface	361:367	arg1	glycans					369:375	cell surface glycans	356:375	cell surface glycans	356:375	This process can be blocked by synthetic carbohydrates that mimic the structure of cell surface glycans.
34095846	0	20	gly	glycosylated	44:55	arg1	inhibitors					57:66	glycosylated inhibitors	44:66	glycosylated inhibitors of fungal adhesion	44:85	Scaffold diversity for enhanced activity of glycosylated inhibitors of fungal adhesion.
34095846	5	21	theme	buccal	747:752	arg1	cells					765:769	buccal epithelial cells	747:769	buccal epithelial cells	747:769	Galactosides 1 and 6, built on an aromatic core, were most efficient inhibitors of adhesion of C. albicans to buccal epithelial cells, displacing up to 36% and 48%, respectively, of yeast already attached to epithelial cells at 138 μM.
34095846	4	22	theme	molecule	597:604	arg1	glycoconjugates					620:634	small molecule anti-adhesion glycoconjugates	591:634	small molecule anti-adhesion glycoconjugates	591:634	Herein we report the evaluation of a series of divalent glycosides featuring aromatic (benzene, squaramide) and bicyclic aliphatic (norbornene) scaffolds, with the latter being the first examples of their kind as small molecule anti-adhesion glycoconjugates.
34095846	3	23	theme	glycans	369:375	arg1	structure					343:351	the structure	339:351	the structure of cell surface glycans	339:375	This process can be blocked by synthetic carbohydrates that mimic the structure of cell surface glycans.
34095846	5	24	theme	aromatic	671:678	arg1	core					680:683	an aromatic core	668:683	an aromatic core	668:683	Galactosides 1 and 6, built on an aromatic core, were most efficient inhibitors of adhesion of C. albicans to buccal epithelial cells, displacing up to 36% and 48%, respectively, of yeast already attached to epithelial cells at 138 μM.
34095846	4	25	theme	glycosides	434:443	arg1	series					415:420	a series	413:420	a series of divalent glycosides featuring aromatic (benzene, squaramide) and bicyclic aliphatic (norbornene) scaffolds	413:530	Herein we report the evaluation of a series of divalent glycosides featuring aromatic (benzene, squaramide) and bicyclic aliphatic (norbornene) scaffolds, with the latter being the first examples of their kind as small molecule anti-adhesion glycoconjugates.
34095846	4	26	theme	small	591:595	arg1	glycoconjugates					620:634	small molecule anti-adhesion glycoconjugates	591:634	small molecule anti-adhesion glycoconjugates	591:634	Herein we report the evaluation of a series of divalent glycosides featuring aromatic (benzene, squaramide) and bicyclic aliphatic (norbornene) scaffolds, with the latter being the first examples of their kind as small molecule anti-adhesion glycoconjugates.
34095846	5	27	theme	albicans	735:742	arg1	adhesion					720:727	adhesion	720:727	adhesion of C. albicans to buccal epithelial cells	720:769	Galactosides 1 and 6, built on an aromatic core, were most efficient inhibitors of adhesion of C. albicans to buccal epithelial cells, displacing up to 36% and 48%, respectively, of yeast already attached to epithelial cells at 138 μM.
34095846	4	28	theme	divalent	425:432	arg1	glycosides					434:443	divalent glycosides	425:443	divalent glycosides featuring aromatic (benzene, squaramide) and bicyclic aliphatic (norbornene) scaffolds	425:530	Herein we report the evaluation of a series of divalent glycosides featuring aromatic (benzene, squaramide) and bicyclic aliphatic (norbornene) scaffolds, with the latter being the first examples of their kind as small molecule anti-adhesion glycoconjugates.
34095846	4	29	theme	aromatic	455:462	arg1	scaffolds					522:530	aromatic (benzene, squaramide) and bicyclic aliphatic (norbornene) scaffolds	455:530	aromatic (benzene, squaramide) and bicyclic aliphatic (norbornene) scaffolds	455:530	Herein we report the evaluation of a series of divalent glycosides featuring aromatic (benzene, squaramide) and bicyclic aliphatic (norbornene) scaffolds, with the latter being the first examples of their kind as small molecule anti-adhesion glycoconjugates.
34095846	4	29	theme	aromatic	455:462	arg1	norbornene					510:519	norbornene	510:519	norbornene	510:519	Herein we report the evaluation of a series of divalent glycosides featuring aromatic (benzene, squaramide) and bicyclic aliphatic (norbornene) scaffolds, with the latter being the first examples of their kind as small molecule anti-adhesion glycoconjugates.
34095846	0	30	theme	enhanced	23:30	arg1	activity					32:39	enhanced activity	23:39	enhanced activity of glycosylated inhibitors of fungal adhesion	23:85	Scaffold diversity for enhanced activity of glycosylated inhibitors of fungal adhesion.
34095846	7	31	theme	Conformational	958:971	arg1	analysis					973:980	Conformational analysis	958:980	Conformational analysis	958:980	Conformational analysis reveals a preference for compounds 1 and 21 to adopt folded conformations.
34095846	4	32	theme	anti-adhesion	606:618	arg1	glycoconjugates					620:634	small molecule anti-adhesion glycoconjugates	591:634	small molecule anti-adhesion glycoconjugates	591:634	Herein we report the evaluation of a series of divalent glycosides featuring aromatic (benzene, squaramide) and bicyclic aliphatic (norbornene) scaffolds, with the latter being the first examples of their kind as small molecule anti-adhesion glycoconjugates.
34095846	0	33	theme	inhibitors	57:66	arg1	activity					32:39	enhanced activity	23:39	enhanced activity of glycosylated inhibitors of fungal adhesion	23:85	Scaffold diversity for enhanced activity of glycosylated inhibitors of fungal adhesion.
34095846	0	34	theme	glycosylated	44:55	arg1	inhibitors					57:66	glycosylated inhibitors	44:66	glycosylated inhibitors of fungal adhesion	44:85	Scaffold diversity for enhanced activity of glycosylated inhibitors of fungal adhesion.
34095846	5	35	dep	36	789:790	arg1	to					786:787	to	786:787	to	786:787	Galactosides 1 and 6, built on an aromatic core, were most efficient inhibitors of adhesion of C. albicans to buccal epithelial cells, displacing up to 36% and 48%, respectively, of yeast already attached to epithelial cells at 138 μM.
34095846	5	36	theme	epithelial	845:854	arg1	cells					856:860	epithelial cells	845:860	epithelial cells	845:860	Galactosides 1 and 6, built on an aromatic core, were most efficient inhibitors of adhesion of C. albicans to buccal epithelial cells, displacing up to 36% and 48%, respectively, of yeast already attached to epithelial cells at 138 μM.
34095846	1	37	theme	prevalent	124:132	arg1	pathogens					141:149	the most prevalent fungal pathogens	115:149	the most prevalent fungal pathogens involved in hospital acquired infections	115:190	Candida albicans is one of the most prevalent fungal pathogens involved in hospital acquired infections.
34095846	8	38	theme	anti-adhesion	1169:1181	arg1	compounds					1183:1191	anti-adhesion compounds	1169:1191	anti-adhesion compounds	1169:1191	These results highlight the potential of norbornenes as a new class of aliphatic scaffolds for the synthesis of anti-adhesion compounds.
34095846	0	39	theme	fungal	71:76	arg1	adhesion					78:85	fungal adhesion	71:85	fungal adhesion	71:85	Scaffold diversity for enhanced activity of glycosylated inhibitors of fungal adhesion.
34095846	4	40	theme	series	415:420	arg1	evaluation					399:408	the evaluation	395:408	the evaluation of a series of divalent glycosides featuring aromatic (benzene, squaramide) and bicyclic aliphatic (norbornene) scaffolds	395:530	Herein we report the evaluation of a series of divalent glycosides featuring aromatic (benzene, squaramide) and bicyclic aliphatic (norbornene) scaffolds, with the latter being the first examples of their kind as small molecule anti-adhesion glycoconjugates.
34095846	2	41	theme	cells	242:246	arg1	surface					220:226	the surface	216:226	the surface of epithelial cells	216:246	It binds to glycans at the surface of epithelial cells and initiates infection.
34095846	1	42	theme	fungal	134:139	arg1	pathogens					141:149	the most prevalent fungal pathogens	115:149	the most prevalent fungal pathogens involved in hospital acquired infections	115:190	Candida albicans is one of the most prevalent fungal pathogens involved in hospital acquired infections.
34095846	8	43	theme	compounds	1183:1191	arg1	synthesis					1156:1164	the synthesis	1152:1164	the synthesis of anti-adhesion compounds	1152:1191	These results highlight the potential of norbornenes as a new class of aliphatic scaffolds for the synthesis of anti-adhesion compounds.
34095846	5	44	theme	adhesion	720:727	arg1	inhibitors					706:715	most efficient inhibitors	691:715	most efficient inhibitors of adhesion of C. albicans to buccal epithelial cells	691:769	Galactosides 1 and 6, built on an aromatic core, were most efficient inhibitors of adhesion of C. albicans to buccal epithelial cells, displacing up to 36% and 48%, respectively, of yeast already attached to epithelial cells at 138 μM.
34095846	2	45	theme	epithelial	231:240	arg1	cells					242:246	epithelial cells	231:246	epithelial cells	231:246	It binds to glycans at the surface of epithelial cells and initiates infection.
34095846	1	46	theme	pathogens	141:149	arg1	pathogens					141:149	the most prevalent fungal pathogens	115:149	the most prevalent fungal pathogens involved in hospital acquired infections	115:190	Candida albicans is one of the most prevalent fungal pathogens involved in hospital acquired infections.
34095846	1	46	theme	pathogens	141:149	arg1	one					108:110	one	108:110	one	108:110	Candida albicans is one of the most prevalent fungal pathogens involved in hospital acquired infections.
34095846	4	47	theme	kind	583:586	arg1	examples					565:572	the first examples	555:572	the first examples of their kind	555:586	Herein we report the evaluation of a series of divalent glycosides featuring aromatic (benzene, squaramide) and bicyclic aliphatic (norbornene) scaffolds, with the latter being the first examples of their kind as small molecule anti-adhesion glycoconjugates.
34095846	4	47	theme	kind	583:586	arg1	latter					542:547	latter	542:547	latter	542:547	Herein we report the evaluation of a series of divalent glycosides featuring aromatic (benzene, squaramide) and bicyclic aliphatic (norbornene) scaffolds, with the latter being the first examples of their kind as small molecule anti-adhesion glycoconjugates.
32495171	6	0	dep	mycobacteria	926:937	arg1	mycobacteria					926:937	captured mycobacteria tuberculosis, dextrazide, and dextrazide-loaded liposomes	917:995	captured mycobacteria tuberculosis, dextrazide, and dextrazide-loaded liposomes	917:995	Macrophages with captured mycobacteria tuberculosis, dextrazide, and dextrazide-loaded liposomes can be incorporated into granulomas.
32495171	6	0	dep	mycobacteria	926:937	arg1	dextrazide					953:962	dextrazide	953:962	dextrazide	953:962	Macrophages with captured mycobacteria tuberculosis, dextrazide, and dextrazide-loaded liposomes can be incorporated into granulomas.
32495171	6	0	dep	mycobacteria	926:937	arg1	tuberculosis					939:950	tuberculosis	939:950	tuberculosis	939:950	Macrophages with captured mycobacteria tuberculosis, dextrazide, and dextrazide-loaded liposomes can be incorporated into granulomas.
32495171	6	0	dep	mycobacteria	926:937	arg1	liposomes					987:995	dextrazide-loaded liposomes	969:995	dextrazide-loaded liposomes	969:995	Macrophages with captured mycobacteria tuberculosis, dextrazide, and dextrazide-loaded liposomes can be incorporated into granulomas.
32495171	1	1	theme	BALB/c	337:342	arg1	mice					344:347	BALB/c mice	337:347	BALB/c mice	337:347	The study examined effectiveness of liposomal form of dextrazide (inhaled or intraperitoneal), free dextrazide (intraperitoneal), and isoniazid (intraperitoneal) in the treatment of BALB/c mice with BCG-induced granulomatosis.
32495171	7	2	theme	prodestructive	1184:1197	arg1	potential					1199:1207	the prodestructive potential	1180:1207	the prodestructive potential of lysosomes in macrophages realized after their migration from granulomas	1180:1282	The antimycobacterial effect of dextrazide is an important factor preventing the destructive processes in granulomas and organs via a decrease in the prodestructive potential of lysosomes in macrophages realized after their migration from granulomas.
32495171	7	3	theme	antimycobacterial	1038:1054	arg1	effect					1056:1061	The antimycobacterial effect	1034:1061	The antimycobacterial effect of dextrazide	1034:1075	The antimycobacterial effect of dextrazide is an important factor preventing the destructive processes in granulomas and organs via a decrease in the prodestructive potential of lysosomes in macrophages realized after their migration from granulomas.
32495171	7	3	theme	antimycobacterial	1038:1054	arg1	factor					1093:1098	an important factor	1080:1098	an important factor preventing the destructive processes in granulomas and organs via a decrease in the prodestructive potential of lysosomes in macrophages realized after their migration from granulomas	1080:1282	The antimycobacterial effect of dextrazide is an important factor preventing the destructive processes in granulomas and organs via a decrease in the prodestructive potential of lysosomes in macrophages realized after their migration from granulomas.
32495171	4	4	dep	number	576:581	arg1	the					572:574	the	572:574	the	572:574	The decrease of the number and size of macrophagal granulomas in mice BCG-induced granulomatosis during treatment was determined by the number of living mycobacteria tuberculosis in these granulomas.
32495171	4	5	theme	size	587:590	arg1	decrease					560:567	The decrease	556:567	The decrease of the number and size of macrophagal granulomas in mice BCG-induced granulomatosis during treatment	556:668	The decrease of the number and size of macrophagal granulomas in mice BCG-induced granulomatosis during treatment was determined by the number of living mycobacteria tuberculosis in these granulomas.
32495171	0	6	theme	Compositions	88:99	arg1	Location					0:7	Location	0:7	Location of Pulmonary Mycobacteria Tuberculosis and Effectiveness of Various Dextrazide Compositions in Treatment of Mice with BCG-Induced Granulomatosis.	0:153	Location of Pulmonary Mycobacteria Tuberculosis and Effectiveness of Various Dextrazide Compositions in Treatment of Mice with BCG-Induced Granulomatosis.
32495171	1	7	theme	mice	344:347	arg1	treatment					324:332	the treatment	320:332	the treatment of BALB/c mice with BCG-induced granulomatosis	320:379	The study examined effectiveness of liposomal form of dextrazide (inhaled or intraperitoneal), free dextrazide (intraperitoneal), and isoniazid (intraperitoneal) in the treatment of BALB/c mice with BCG-induced granulomatosis.
32495171	4	8	theme	mycobacteria	709:720	arg1	number					692:697	the number	688:697	the number of living mycobacteria tuberculosis in these granulomas	688:753	The decrease of the number and size of macrophagal granulomas in mice BCG-induced granulomatosis during treatment was determined by the number of living mycobacteria tuberculosis in these granulomas.
32495171	1	9	dep	isoniazid	289:297	arg1	intraperitoneal					300:314	intraperitoneal	300:314	intraperitoneal	300:314	The study examined effectiveness of liposomal form of dextrazide (inhaled or intraperitoneal), free dextrazide (intraperitoneal), and isoniazid (intraperitoneal) in the treatment of BALB/c mice with BCG-induced granulomatosis.
32495171	1	10	with	treatment	324:332	arg1	granulomatosis					366:379	BCG-induced granulomatosis	354:379	BCG-induced granulomatosis	354:379	The study examined effectiveness of liposomal form of dextrazide (inhaled or intraperitoneal), free dextrazide (intraperitoneal), and isoniazid (intraperitoneal) in the treatment of BALB/c mice with BCG-induced granulomatosis.
32495171	7	11	from	granulomas	1273:1282	arg1	migration					1258:1266	their migration	1252:1266	their migration from granulomas	1252:1282	The antimycobacterial effect of dextrazide is an important factor preventing the destructive processes in granulomas and organs via a decrease in the prodestructive potential of lysosomes in macrophages realized after their migration from granulomas.
32495171	4	12	theme	macrophagal	595:605	arg1	granulomas					607:616	macrophagal granulomas	595:616	macrophagal granulomas in mice BCG-induced granulomatosis	595:651	The decrease of the number and size of macrophagal granulomas in mice BCG-induced granulomatosis during treatment was determined by the number of living mycobacteria tuberculosis in these granulomas.
32495171	1	13	theme	BCG-induced	354:364	arg1	granulomatosis					366:379	BCG-induced granulomatosis	354:379	BCG-induced granulomatosis	354:379	The study examined effectiveness of liposomal form of dextrazide (inhaled or intraperitoneal), free dextrazide (intraperitoneal), and isoniazid (intraperitoneal) in the treatment of BALB/c mice with BCG-induced granulomatosis.
32495171	4	14	dep	mycobacteria	709:720	arg1	tuberculosis					722:733	tuberculosis	722:733	living mycobacteria tuberculosis in these granulomas	702:753	The decrease of the number and size of macrophagal granulomas in mice BCG-induced granulomatosis during treatment was determined by the number of living mycobacteria tuberculosis in these granulomas.
32495171	5	15	theme	isonicotinic	870:881	arg1	hydrazide					888:896	isonicotinic acid hydrazide	870:896	isonicotinic acid hydrazide	870:896	The most effective treatment was achieved with liposomal form of dextrazide (a conjugate of oxidized dextran with isonicotinic acid hydrazide).
32495171	4	16	from	size	587:590	arg1	mice					621:624	mice	621:624	mice BCG-induced granulomatosis	621:651	The decrease of the number and size of macrophagal granulomas in mice BCG-induced granulomatosis during treatment was determined by the number of living mycobacteria tuberculosis in these granulomas.
32495171	5	17	dep	dextrazide	821:830	arg1	conjugate					835:843	a conjugate	833:843	a conjugate of oxidized dextran with isonicotinic acid hydrazide	833:896	The most effective treatment was achieved with liposomal form of dextrazide (a conjugate of oxidized dextran with isonicotinic acid hydrazide).
32495171	1	18	theme	liposomal	191:199	arg1	form					201:204	liposomal form	191:204	liposomal form of dextrazide (inhaled or intraperitoneal), free dextrazide (intraperitoneal), and isoniazid (intraperitoneal)	191:315	The study examined effectiveness of liposomal form of dextrazide (inhaled or intraperitoneal), free dextrazide (intraperitoneal), and isoniazid (intraperitoneal) in the treatment of BALB/c mice with BCG-induced granulomatosis.
32495171	0	19	theme	Mice	117:120	arg1	Treatment					104:112	Treatment	104:112	Treatment of Mice with BCG-Induced Granulomatosis	104:152	Location of Pulmonary Mycobacteria Tuberculosis and Effectiveness of Various Dextrazide Compositions in Treatment of Mice with BCG-Induced Granulomatosis.
32495171	6	20	theme	captured	917:924	arg1	mycobacteria					926:937	captured mycobacteria tuberculosis, dextrazide, and dextrazide-loaded liposomes	917:995	captured mycobacteria tuberculosis, dextrazide, and dextrazide-loaded liposomes	917:995	Macrophages with captured mycobacteria tuberculosis, dextrazide, and dextrazide-loaded liposomes can be incorporated into granulomas.
32495171	6	20	theme	captured	917:924	arg1	dextrazide					953:962	dextrazide	953:962	dextrazide	953:962	Macrophages with captured mycobacteria tuberculosis, dextrazide, and dextrazide-loaded liposomes can be incorporated into granulomas.
32495171	6	20	theme	captured	917:924	arg1	tuberculosis					939:950	tuberculosis	939:950	tuberculosis	939:950	Macrophages with captured mycobacteria tuberculosis, dextrazide, and dextrazide-loaded liposomes can be incorporated into granulomas.
32495171	7	21	from	processes	1127:1135	arg1	organs					1155:1160	organs	1155:1160	organs	1155:1160	The antimycobacterial effect of dextrazide is an important factor preventing the destructive processes in granulomas and organs via a decrease in the prodestructive potential of lysosomes in macrophages realized after their migration from granulomas.
32495171	7	21	from	processes	1127:1135	arg1	granulomas					1140:1149	granulomas	1140:1149	granulomas	1140:1149	The antimycobacterial effect of dextrazide is an important factor preventing the destructive processes in granulomas and organs via a decrease in the prodestructive potential of lysosomes in macrophages realized after their migration from granulomas.
32495171	1	22	theme	form	201:204	arg1	effectiveness					174:186	effectiveness	174:186	effectiveness of liposomal form of dextrazide (inhaled or intraperitoneal), free dextrazide (intraperitoneal), and isoniazid (intraperitoneal)	174:315	The study examined effectiveness of liposomal form of dextrazide (inhaled or intraperitoneal), free dextrazide (intraperitoneal), and isoniazid (intraperitoneal) in the treatment of BALB/c mice with BCG-induced granulomatosis.
32495171	4	23	from	number	576:581	arg1	mice					621:624	mice	621:624	mice BCG-induced granulomatosis	621:651	The decrease of the number and size of macrophagal granulomas in mice BCG-induced granulomatosis during treatment was determined by the number of living mycobacteria tuberculosis in these granulomas.
32495171	4	24	from	granulomas	607:616	arg1	mice					621:624	mice	621:624	mice BCG-induced granulomatosis	621:651	The decrease of the number and size of macrophagal granulomas in mice BCG-induced granulomatosis during treatment was determined by the number of living mycobacteria tuberculosis in these granulomas.
32495171	6	25	with	Macrophages	900:910	arg1	mycobacteria					926:937	captured mycobacteria tuberculosis, dextrazide, and dextrazide-loaded liposomes	917:995	captured mycobacteria tuberculosis, dextrazide, and dextrazide-loaded liposomes	917:995	Macrophages with captured mycobacteria tuberculosis, dextrazide, and dextrazide-loaded liposomes can be incorporated into granulomas.
32495171	6	25	with	Macrophages	900:910	arg1	dextrazide					953:962	dextrazide	953:962	dextrazide	953:962	Macrophages with captured mycobacteria tuberculosis, dextrazide, and dextrazide-loaded liposomes can be incorporated into granulomas.
32495171	6	25	with	Macrophages	900:910	arg1	tuberculosis					939:950	tuberculosis	939:950	tuberculosis	939:950	Macrophages with captured mycobacteria tuberculosis, dextrazide, and dextrazide-loaded liposomes can be incorporated into granulomas.
32495171	7	26	from	macrophages	1225:1235	arg1	potential					1199:1207	the prodestructive potential	1180:1207	the prodestructive potential of lysosomes in macrophages realized after their migration from granulomas	1180:1282	The antimycobacterial effect of dextrazide is an important factor preventing the destructive processes in granulomas and organs via a decrease in the prodestructive potential of lysosomes in macrophages realized after their migration from granulomas.
32495171	4	27	dep	BCG-induced	626:636	arg1	granulomatosis					638:651	granulomatosis	638:651	granulomatosis	638:651	The decrease of the number and size of macrophagal granulomas in mice BCG-induced granulomatosis during treatment was determined by the number of living mycobacteria tuberculosis in these granulomas.
32495171	1	28	theme	dextrazide	209:218	arg1	form					201:204	liposomal form	191:204	liposomal form of dextrazide (inhaled or intraperitoneal), free dextrazide (intraperitoneal), and isoniazid (intraperitoneal)	191:315	The study examined effectiveness of liposomal form of dextrazide (inhaled or intraperitoneal), free dextrazide (intraperitoneal), and isoniazid (intraperitoneal) in the treatment of BALB/c mice with BCG-induced granulomatosis.
32495171	0	29	theme	Mycobacteria	22:33	arg1	Location					0:7	Location	0:7	Location of Pulmonary Mycobacteria Tuberculosis and Effectiveness of Various Dextrazide Compositions in Treatment of Mice with BCG-Induced Granulomatosis.	0:153	Location of Pulmonary Mycobacteria Tuberculosis and Effectiveness of Various Dextrazide Compositions in Treatment of Mice with BCG-Induced Granulomatosis.
32495171	1	30	theme	isoniazid	289:297	arg1	form					201:204	liposomal form	191:204	liposomal form of dextrazide (inhaled or intraperitoneal), free dextrazide (intraperitoneal), and isoniazid (intraperitoneal)	191:315	The study examined effectiveness of liposomal form of dextrazide (inhaled or intraperitoneal), free dextrazide (intraperitoneal), and isoniazid (intraperitoneal) in the treatment of BALB/c mice with BCG-induced granulomatosis.
32495171	0	31	with	Treatment	104:112	arg1	Granulomatosis					139:152	BCG-Induced Granulomatosis	127:152	BCG-Induced Granulomatosis	127:152	Location of Pulmonary Mycobacteria Tuberculosis and Effectiveness of Various Dextrazide Compositions in Treatment of Mice with BCG-Induced Granulomatosis.
32495171	5	32	theme	dextran	857:863	arg1	conjugate					835:843	a conjugate	833:843	a conjugate of oxidized dextran with isonicotinic acid hydrazide	833:896	The most effective treatment was achieved with liposomal form of dextrazide (a conjugate of oxidized dextran with isonicotinic acid hydrazide).
32495171	0	33	theme	Pulmonary	12:20	arg1	Mycobacteria					22:33	Pulmonary Mycobacteria Tuberculosis and Effectiveness	12:64	Pulmonary Mycobacteria Tuberculosis and Effectiveness	12:64	Location of Pulmonary Mycobacteria Tuberculosis and Effectiveness of Various Dextrazide Compositions in Treatment of Mice with BCG-Induced Granulomatosis.
32495171	0	33	theme	Pulmonary	12:20	arg1	Effectiveness					52:64	Effectiveness	52:64	Effectiveness	52:64	Location of Pulmonary Mycobacteria Tuberculosis and Effectiveness of Various Dextrazide Compositions in Treatment of Mice with BCG-Induced Granulomatosis.
32495171	0	33	theme	Pulmonary	12:20	arg1	Tuberculosis					35:46	Tuberculosis	35:46	Tuberculosis	35:46	Location of Pulmonary Mycobacteria Tuberculosis and Effectiveness of Various Dextrazide Compositions in Treatment of Mice with BCG-Induced Granulomatosis.
32495171	4	34	theme	number	576:581	arg1	decrease					560:567	The decrease	556:567	The decrease of the number and size of macrophagal granulomas in mice BCG-induced granulomatosis during treatment	556:668	The decrease of the number and size of macrophagal granulomas in mice BCG-induced granulomatosis during treatment was determined by the number of living mycobacteria tuberculosis in these granulomas.
32495171	0	35	theme	BCG-Induced	127:137	arg1	Granulomatosis					139:152	BCG-Induced Granulomatosis	127:152	BCG-Induced Granulomatosis	127:152	Location of Pulmonary Mycobacteria Tuberculosis and Effectiveness of Various Dextrazide Compositions in Treatment of Mice with BCG-Induced Granulomatosis.
32495171	5	36	with	conjugate	835:843	arg1	hydrazide					888:896	isonicotinic acid hydrazide	870:896	isonicotinic acid hydrazide	870:896	The most effective treatment was achieved with liposomal form of dextrazide (a conjugate of oxidized dextran with isonicotinic acid hydrazide).
32495171	7	37	from	decrease	1168:1175	arg1	potential					1199:1207	the prodestructive potential	1180:1207	the prodestructive potential of lysosomes in macrophages realized after their migration from granulomas	1180:1282	The antimycobacterial effect of dextrazide is an important factor preventing the destructive processes in granulomas and organs via a decrease in the prodestructive potential of lysosomes in macrophages realized after their migration from granulomas.
32495171	1	38	dep	dextrazide	255:264	arg1	intraperitoneal					267:281	intraperitoneal	267:281	intraperitoneal	267:281	The study examined effectiveness of liposomal form of dextrazide (inhaled or intraperitoneal), free dextrazide (intraperitoneal), and isoniazid (intraperitoneal) in the treatment of BALB/c mice with BCG-induced granulomatosis.
32495171	2	39	theme	tuberculosis	423:434	arg1	months					438:443	mycobacteria tuberculosis 3 months	410:443	mycobacteria tuberculosis 3 months prior to onset of treatment	410:471	The mice were infected with mycobacteria tuberculosis 3 months prior to onset of treatment.
32495171	5	40	theme	liposomal	803:811	arg1	form					813:816	liposomal form	803:816	liposomal form of dextrazide (a conjugate of oxidized dextran with isonicotinic acid hydrazide)	803:897	The most effective treatment was achieved with liposomal form of dextrazide (a conjugate of oxidized dextran with isonicotinic acid hydrazide).
32495171	1	41	dep	dextrazide	209:218	arg1	inhaled					221:227	inhaled	221:227	inhaled	221:227	The study examined effectiveness of liposomal form of dextrazide (inhaled or intraperitoneal), free dextrazide (intraperitoneal), and isoniazid (intraperitoneal) in the treatment of BALB/c mice with BCG-induced granulomatosis.
32495171	1	41	dep	dextrazide	209:218	arg1	intraperitoneal					232:246	intraperitoneal	232:246	intraperitoneal	232:246	The study examined effectiveness of liposomal form of dextrazide (inhaled or intraperitoneal), free dextrazide (intraperitoneal), and isoniazid (intraperitoneal) in the treatment of BALB/c mice with BCG-induced granulomatosis.
32495171	2	42	theme	mycobacteria	410:421	arg1	months					438:443	mycobacteria tuberculosis 3 months	410:443	mycobacteria tuberculosis 3 months prior to onset of treatment	410:471	The mice were infected with mycobacteria tuberculosis 3 months prior to onset of treatment.
32495171	5	43	theme	dextrazide	821:830	arg1	form					813:816	liposomal form	803:816	liposomal form of dextrazide (a conjugate of oxidized dextran with isonicotinic acid hydrazide)	803:897	The most effective treatment was achieved with liposomal form of dextrazide (a conjugate of oxidized dextran with isonicotinic acid hydrazide).
32495171	7	44	from	potential	1199:1207	arg1	macrophages					1225:1235	macrophages	1225:1235	macrophages realized after their migration from granulomas	1225:1282	The antimycobacterial effect of dextrazide is an important factor preventing the destructive processes in granulomas and organs via a decrease in the prodestructive potential of lysosomes in macrophages realized after their migration from granulomas.
32495171	4	45	from	mice	621:624	arg1	size					587:590	size	587:590	size	587:590	The decrease of the number and size of macrophagal granulomas in mice BCG-induced granulomatosis during treatment was determined by the number of living mycobacteria tuberculosis in these granulomas.
32495171	4	45	from	mice	621:624	arg1	number					576:581	number	576:581	number	576:581	The decrease of the number and size of macrophagal granulomas in mice BCG-induced granulomatosis during treatment was determined by the number of living mycobacteria tuberculosis in these granulomas.
32495171	7	46	theme	important	1083:1091	arg1	effect					1056:1061	The antimycobacterial effect	1034:1061	The antimycobacterial effect of dextrazide	1034:1075	The antimycobacterial effect of dextrazide is an important factor preventing the destructive processes in granulomas and organs via a decrease in the prodestructive potential of lysosomes in macrophages realized after their migration from granulomas.
32495171	7	46	theme	important	1083:1091	arg1	factor					1093:1098	an important factor	1080:1098	an important factor preventing the destructive processes in granulomas and organs via a decrease in the prodestructive potential of lysosomes in macrophages realized after their migration from granulomas	1080:1282	The antimycobacterial effect of dextrazide is an important factor preventing the destructive processes in granulomas and organs via a decrease in the prodestructive potential of lysosomes in macrophages realized after their migration from granulomas.
32495171	0	47	from	Location	0:7	arg1	Treatment					104:112	Treatment	104:112	Treatment of Mice with BCG-Induced Granulomatosis	104:152	Location of Pulmonary Mycobacteria Tuberculosis and Effectiveness of Various Dextrazide Compositions in Treatment of Mice with BCG-Induced Granulomatosis.
32495171	7	48	theme	destructive	1115:1125	arg1	processes					1127:1135	the destructive processes	1111:1135	the destructive processes in granulomas and organs	1111:1160	The antimycobacterial effect of dextrazide is an important factor preventing the destructive processes in granulomas and organs via a decrease in the prodestructive potential of lysosomes in macrophages realized after their migration from granulomas.
32495171	2	49	theme	treatment	463:471	arg1	onset					454:458	onset	454:458	onset of treatment	454:471	The mice were infected with mycobacteria tuberculosis 3 months prior to onset of treatment.
32495171	7	50	theme	dextrazide	1066:1075	arg1	effect					1056:1061	The antimycobacterial effect	1034:1061	The antimycobacterial effect of dextrazide	1034:1075	The antimycobacterial effect of dextrazide is an important factor preventing the destructive processes in granulomas and organs via a decrease in the prodestructive potential of lysosomes in macrophages realized after their migration from granulomas.
32495171	7	50	theme	dextrazide	1066:1075	arg1	factor					1093:1098	an important factor	1080:1098	an important factor preventing the destructive processes in granulomas and organs via a decrease in the prodestructive potential of lysosomes in macrophages realized after their migration from granulomas	1080:1282	The antimycobacterial effect of dextrazide is an important factor preventing the destructive processes in granulomas and organs via a decrease in the prodestructive potential of lysosomes in macrophages realized after their migration from granulomas.
32495171	6	51	theme	dextrazide-loaded	969:985	arg1	liposomes					987:995	dextrazide-loaded liposomes	969:995	dextrazide-loaded liposomes	969:995	Macrophages with captured mycobacteria tuberculosis, dextrazide, and dextrazide-loaded liposomes can be incorporated into granulomas.
32495171	7	52	theme	lysosomes	1212:1220	arg1	potential					1199:1207	the prodestructive potential	1180:1207	the prodestructive potential of lysosomes in macrophages realized after their migration from granulomas	1180:1282	The antimycobacterial effect of dextrazide is an important factor preventing the destructive processes in granulomas and organs via a decrease in the prodestructive potential of lysosomes in macrophages realized after their migration from granulomas.
32495171	0	53	dep	Mycobacteria	22:33	arg1	Mycobacteria					22:33	Pulmonary Mycobacteria Tuberculosis and Effectiveness	12:64	Pulmonary Mycobacteria Tuberculosis and Effectiveness	12:64	Location of Pulmonary Mycobacteria Tuberculosis and Effectiveness of Various Dextrazide Compositions in Treatment of Mice with BCG-Induced Granulomatosis.
32495171	0	53	dep	Mycobacteria	22:33	arg1	Effectiveness					52:64	Effectiveness	52:64	Effectiveness	52:64	Location of Pulmonary Mycobacteria Tuberculosis and Effectiveness of Various Dextrazide Compositions in Treatment of Mice with BCG-Induced Granulomatosis.
32495171	0	53	dep	Mycobacteria	22:33	arg1	Tuberculosis					35:46	Tuberculosis	35:46	Tuberculosis	35:46	Location of Pulmonary Mycobacteria Tuberculosis and Effectiveness of Various Dextrazide Compositions in Treatment of Mice with BCG-Induced Granulomatosis.
32495171	7	54	from	lysosomes	1212:1220	arg1	macrophages					1225:1235	macrophages	1225:1235	macrophages realized after their migration from granulomas	1225:1282	The antimycobacterial effect of dextrazide is an important factor preventing the destructive processes in granulomas and organs via a decrease in the prodestructive potential of lysosomes in macrophages realized after their migration from granulomas.
32495171	5	55	theme	effective	765:773	arg1	treatment					775:783	The most effective treatment	756:783	The most effective treatment	756:783	The most effective treatment was achieved with liposomal form of dextrazide (a conjugate of oxidized dextran with isonicotinic acid hydrazide).
32495171	5	56	theme	oxidized	848:855	arg1	dextran					857:863	oxidized dextran	848:863	oxidized dextran	848:863	The most effective treatment was achieved with liposomal form of dextrazide (a conjugate of oxidized dextran with isonicotinic acid hydrazide).
32495171	4	57	theme	granulomas	607:616	arg1	size					587:590	size	587:590	size	587:590	The decrease of the number and size of macrophagal granulomas in mice BCG-induced granulomatosis during treatment was determined by the number of living mycobacteria tuberculosis in these granulomas.
32495171	4	57	theme	granulomas	607:616	arg1	number					576:581	number	576:581	number	576:581	The decrease of the number and size of macrophagal granulomas in mice BCG-induced granulomatosis during treatment was determined by the number of living mycobacteria tuberculosis in these granulomas.
32495171	0	58	theme	Dextrazide	77:86	arg1	Compositions					88:99	Various Dextrazide Compositions	69:99	Various Dextrazide Compositions	69:99	Location of Pulmonary Mycobacteria Tuberculosis and Effectiveness of Various Dextrazide Compositions in Treatment of Mice with BCG-Induced Granulomatosis.
32495171	5	59	theme	acid	883:886	arg1	hydrazide					888:896	isonicotinic acid hydrazide	870:896	isonicotinic acid hydrazide	870:896	The most effective treatment was achieved with liposomal form of dextrazide (a conjugate of oxidized dextran with isonicotinic acid hydrazide).
32495171	4	60	theme	BCG-induced	626:636	arg1	mice					621:624	mice	621:624	mice BCG-induced granulomatosis	621:651	The decrease of the number and size of macrophagal granulomas in mice BCG-induced granulomatosis during treatment was determined by the number of living mycobacteria tuberculosis in these granulomas.
32495171	4	61	theme	living	702:707	arg1	mycobacteria					709:720	living mycobacteria tuberculosis in these granulomas	702:753	living mycobacteria tuberculosis in these granulomas	702:753	The decrease of the number and size of macrophagal granulomas in mice BCG-induced granulomatosis during treatment was determined by the number of living mycobacteria tuberculosis in these granulomas.
32495171	1	62	theme	free	250:253	arg1	dextrazide					255:264	free dextrazide	250:264	free dextrazide (intraperitoneal)	250:282	The study examined effectiveness of liposomal form of dextrazide (inhaled or intraperitoneal), free dextrazide (intraperitoneal), and isoniazid (intraperitoneal) in the treatment of BALB/c mice with BCG-induced granulomatosis.
32495171	0	63	theme	Various	69:75	arg1	Compositions					88:99	Various Dextrazide Compositions	69:99	Various Dextrazide Compositions	69:99	Location of Pulmonary Mycobacteria Tuberculosis and Effectiveness of Various Dextrazide Compositions in Treatment of Mice with BCG-Induced Granulomatosis.
32495171	2	64	theme	prior	445:449	arg1	months					438:443	mycobacteria tuberculosis 3 months	410:443	mycobacteria tuberculosis 3 months prior to onset of treatment	410:471	The mice were infected with mycobacteria tuberculosis 3 months prior to onset of treatment.
32495171	4	65	from	tuberculosis	722:733	arg1	granulomas					744:753	these granulomas	738:753	these granulomas	738:753	The decrease of the number and size of macrophagal granulomas in mice BCG-induced granulomatosis during treatment was determined by the number of living mycobacteria tuberculosis in these granulomas.
32495171	1	66	theme	dextrazide	255:264	arg1	form					201:204	liposomal form	191:204	liposomal form of dextrazide (inhaled or intraperitoneal), free dextrazide (intraperitoneal), and isoniazid (intraperitoneal)	191:315	The study examined effectiveness of liposomal form of dextrazide (inhaled or intraperitoneal), free dextrazide (intraperitoneal), and isoniazid (intraperitoneal) in the treatment of BALB/c mice with BCG-induced granulomatosis.
33166084	3	0	theme	Encapsulating	304:316	arg1	biologics					318:326	Encapsulating biologics	304:326	Encapsulating biologics in a protective delivery vector	304:358	Encapsulating biologics in a protective delivery vector has the potential to improve delivery profile and enhance performance.
33166084	5	1	theme	modified	695:702	arg1	polymers					712:719	synthetically modified amylose polymers	681:719	synthetically modified amylose polymers	681:719	Here, the development of biodegradable coacervate microvectors (comprising synthetically modified amylose polymers) is presented, for the delivery of biologic payloads to cells.
33166084	4	2	with	materials	486:494	arg1	potential					513:521	established potential	501:521	established potential for the stabilization of biological molecules, such as proteins and nucleic acids	501:603	Coacervate microdroplets are developed as cell-mimetic materials with established potential for the stabilization of biological molecules, such as proteins and nucleic acids.
33166084	5	3	theme	biologic	756:763	arg1	payloads					765:772	biologic payloads	756:772	biologic payloads	756:772	Here, the development of biodegradable coacervate microvectors (comprising synthetically modified amylose polymers) is presented, for the delivery of biologic payloads to cells.
33166084	8	4	theme	therapies	1574:1582	arg1	research					1538:1545	biomedical research	1527:1545	biomedical research	1527:1545	This study demonstrates the potential of coacervate microdroplets as delivery microvectors for biomedical research and the development of new therapies.
33166084	8	4	theme	therapies	1574:1582	arg1	development					1555:1565	the development	1551:1565	the development of new therapies	1551:1582	This study demonstrates the potential of coacervate microdroplets as delivery microvectors for biomedical research and the development of new therapies.
33166084	5	5	theme	amylose	704:710	arg1	polymers					712:719	synthetically modified amylose polymers	681:719	synthetically modified amylose polymers	681:719	Here, the development of biodegradable coacervate microvectors (comprising synthetically modified amylose polymers) is presented, for the delivery of biologic payloads to cells.
33166084	8	6	theme	coacervate	1473:1482	arg1	microdroplets					1484:1496	coacervate microdroplets	1473:1496	coacervate microdroplets	1473:1496	This study demonstrates the potential of coacervate microdroplets as delivery microvectors for biomedical research and the development of new therapies.
33166084	5	7	theme	payloads	765:772	arg1	delivery					744:751	the delivery	740:751	the delivery of biologic payloads to cells	740:781	Here, the development of biodegradable coacervate microvectors (comprising synthetically modified amylose polymers) is presented, for the delivery of biologic payloads to cells.
33166084	6	8	theme	biopolymeric	937:948	arg1	structure					950:958	their biopolymeric structure	931:958	their biopolymeric structure	931:958	Amylose-based coacervate microdroplets are stable under physiological conditions (e.g., temperature and ionic strength), are noncytotoxic owing to their biopolymeric structure, spontaneously interacted with the cell membrane, and are able to deliver and release proteinaceous payloads beyond the plasma membrane.
33166084	2	9	theme	stability	272:280	arg1	lack					248:251	the lack	244:251	the lack of pharmacokinetic stability of biologics	244:293	Limitations arise from the lack of pharmacokinetic stability of biologics in vivo.
33166084	3	10	theme	protective	333:342	arg1	vector					353:358	a protective delivery vector	331:358	a protective delivery vector	331:358	Encapsulating biologics in a protective delivery vector has the potential to improve delivery profile and enhance performance.
33166084	5	11	theme	coacervate	645:654	arg1	microvectors					656:667	biodegradable coacervate microvectors	631:667	biodegradable coacervate microvectors (comprising synthetically modified amylose polymers)	631:720	Here, the development of biodegradable coacervate microvectors (comprising synthetically modified amylose polymers) is presented, for the delivery of biologic payloads to cells.
33166084	5	12	theme	biodegradable	631:643	arg1	microvectors					656:667	biodegradable coacervate microvectors	631:667	biodegradable coacervate microvectors (comprising synthetically modified amylose polymers)	631:720	Here, the development of biodegradable coacervate microvectors (comprising synthetically modified amylose polymers) is presented, for the delivery of biologic payloads to cells.
33166084	2	13	theme	pharmacokinetic	256:270	arg1	stability					272:280	pharmacokinetic stability	256:280	pharmacokinetic stability of biologics	256:293	Limitations arise from the lack of pharmacokinetic stability of biologics in vivo.
33166084	7	14	theme	growth	1355:1360	arg1	factor					1362:1367	human bone morphogenetic protein 2 growth factor	1320:1367	human bone morphogenetic protein 2 growth factor	1320:1367	In particular, myoglobin, an oxygen storage and antioxidant protein, is successfully delivered into human mesenchymal stem cells (hMSCs) within 24 h. Furthermore, coacervate microvectors are implemented for the delivery of human bone morphogenetic protein 2 growth factor, inducing differentiation of hMSCs into osteoprogenitor cells.
33166084	3	15	theme	delivery	344:351	arg1	vector					353:358	a protective delivery vector	331:358	a protective delivery vector	331:358	Encapsulating biologics in a protective delivery vector has the potential to improve delivery profile and enhance performance.
33166084	5	16	theme	microvectors	656:667	arg1	development					616:626	the development	612:626	the development of biodegradable coacervate microvectors (comprising synthetically modified amylose polymers)	612:720	Here, the development of biodegradable coacervate microvectors (comprising synthetically modified amylose polymers) is presented, for the delivery of biologic payloads to cells.
33166084	6	17	theme	physiological	840:852	arg1	conditions					854:863	physiological conditions	840:863	physiological conditions (e.g., temperature and ionic strength)	840:902	Amylose-based coacervate microdroplets are stable under physiological conditions (e.g., temperature and ionic strength), are noncytotoxic owing to their biopolymeric structure, spontaneously interacted with the cell membrane, and are able to deliver and release proteinaceous payloads beyond the plasma membrane.
33166084	4	18	theme	established	501:511	arg1	potential					513:521	established potential	501:521	established potential for the stabilization of biological molecules, such as proteins and nucleic acids	501:603	Coacervate microdroplets are developed as cell-mimetic materials with established potential for the stabilization of biological molecules, such as proteins and nucleic acids.
33166084	1	19	theme	limiting	168:175	arg1	extent					91:96	The extent	87:96	The extent to which biologic payloads can be effectively delivered to cells	87:161	The extent to which biologic payloads can be effectively delivered to cells is a limiting factor in the development of new therapies.
33166084	1	19	theme	limiting	168:175	arg1	factor					177:182	a limiting factor	166:182	a limiting factor in the development of new therapies	166:218	The extent to which biologic payloads can be effectively delivered to cells is a limiting factor in the development of new therapies.
33166084	7	20	theme	oxygen	1126:1131	arg1	storage					1133:1139	an oxygen storage and antioxidant protein	1123:1163	storage	1133:1139	In particular, myoglobin, an oxygen storage and antioxidant protein, is successfully delivered into human mesenchymal stem cells (hMSCs) within 24 h. Furthermore, coacervate microvectors are implemented for the delivery of human bone morphogenetic protein 2 growth factor, inducing differentiation of hMSCs into osteoprogenitor cells.
33166084	7	20	theme	oxygen	1126:1131	arg1	myoglobin					1112:1120	myoglobin	1112:1120	myoglobin	1112:1120	In particular, myoglobin, an oxygen storage and antioxidant protein, is successfully delivered into human mesenchymal stem cells (hMSCs) within 24 h. Furthermore, coacervate microvectors are implemented for the delivery of human bone morphogenetic protein 2 growth factor, inducing differentiation of hMSCs into osteoprogenitor cells.
33166084	7	21	theme	factor	1362:1367	arg1	delivery					1308:1315	the delivery	1304:1315	the delivery of human bone morphogenetic protein 2 growth factor	1304:1367	In particular, myoglobin, an oxygen storage and antioxidant protein, is successfully delivered into human mesenchymal stem cells (hMSCs) within 24 h. Furthermore, coacervate microvectors are implemented for the delivery of human bone morphogenetic protein 2 growth factor, inducing differentiation of hMSCs into osteoprogenitor cells.
33166084	7	22	theme	mesenchymal	1203:1213	arg1	hMSCs					1227:1231	hMSCs	1227:1231	hMSCs	1227:1231	In particular, myoglobin, an oxygen storage and antioxidant protein, is successfully delivered into human mesenchymal stem cells (hMSCs) within 24 h. Furthermore, coacervate microvectors are implemented for the delivery of human bone morphogenetic protein 2 growth factor, inducing differentiation of hMSCs into osteoprogenitor cells.
33166084	7	22	theme	mesenchymal	1203:1213	arg1	cells					1220:1224	human mesenchymal stem cells	1197:1224	human mesenchymal stem cells (hMSCs)	1197:1232	In particular, myoglobin, an oxygen storage and antioxidant protein, is successfully delivered into human mesenchymal stem cells (hMSCs) within 24 h. Furthermore, coacervate microvectors are implemented for the delivery of human bone morphogenetic protein 2 growth factor, inducing differentiation of hMSCs into osteoprogenitor cells.
33166084	6	23	dep	temperature	872:882	arg1	e.g.					866:869	e.g.	866:869	e.g.	866:869	Amylose-based coacervate microdroplets are stable under physiological conditions (e.g., temperature and ionic strength), are noncytotoxic owing to their biopolymeric structure, spontaneously interacted with the cell membrane, and are able to deliver and release proteinaceous payloads beyond the plasma membrane.
33166084	7	24	dep	h.	1244:1245	arg1	implemented					1288:1298	implemented	1288:1298	implemented	1288:1298	In particular, myoglobin, an oxygen storage and antioxidant protein, is successfully delivered into human mesenchymal stem cells (hMSCs) within 24 h. Furthermore, coacervate microvectors are implemented for the delivery of human bone morphogenetic protein 2 growth factor, inducing differentiation of hMSCs into osteoprogenitor cells.
33166084	7	25	theme	protein	1345:1351	arg1	factor					1362:1367	human bone morphogenetic protein 2 growth factor	1320:1367	human bone morphogenetic protein 2 growth factor	1320:1367	In particular, myoglobin, an oxygen storage and antioxidant protein, is successfully delivered into human mesenchymal stem cells (hMSCs) within 24 h. Furthermore, coacervate microvectors are implemented for the delivery of human bone morphogenetic protein 2 growth factor, inducing differentiation of hMSCs into osteoprogenitor cells.
33166084	7	26	theme	stem	1215:1218	arg1	hMSCs					1227:1231	hMSCs	1227:1231	hMSCs	1227:1231	In particular, myoglobin, an oxygen storage and antioxidant protein, is successfully delivered into human mesenchymal stem cells (hMSCs) within 24 h. Furthermore, coacervate microvectors are implemented for the delivery of human bone morphogenetic protein 2 growth factor, inducing differentiation of hMSCs into osteoprogenitor cells.
33166084	7	26	theme	stem	1215:1218	arg1	cells					1220:1224	human mesenchymal stem cells	1197:1224	human mesenchymal stem cells (hMSCs)	1197:1232	In particular, myoglobin, an oxygen storage and antioxidant protein, is successfully delivered into human mesenchymal stem cells (hMSCs) within 24 h. Furthermore, coacervate microvectors are implemented for the delivery of human bone morphogenetic protein 2 growth factor, inducing differentiation of hMSCs into osteoprogenitor cells.
33166084	4	27	theme	nucleic	591:597	arg1	acids					599:603	nucleic acids	591:603	nucleic acids	591:603	Coacervate microdroplets are developed as cell-mimetic materials with established potential for the stabilization of biological molecules, such as proteins and nucleic acids.
33166084	6	28	theme	Amylose-based	784:796	arg1	microdroplets					809:821	Amylose-based coacervate microdroplets	784:821	Amylose-based coacervate microdroplets	784:821	Amylose-based coacervate microdroplets are stable under physiological conditions (e.g., temperature and ionic strength), are noncytotoxic owing to their biopolymeric structure, spontaneously interacted with the cell membrane, and are able to deliver and release proteinaceous payloads beyond the plasma membrane.
33166084	2	29	theme	biologics	285:293	arg1	stability					272:280	pharmacokinetic stability	256:280	pharmacokinetic stability of biologics	256:293	Limitations arise from the lack of pharmacokinetic stability of biologics in vivo.
33166084	7	30	theme	morphogenetic	1331:1343	arg1	protein					1345:1351	human bone morphogenetic protein 2	1320:1353	human bone morphogenetic protein 2 growth factor	1320:1367	In particular, myoglobin, an oxygen storage and antioxidant protein, is successfully delivered into human mesenchymal stem cells (hMSCs) within 24 h. Furthermore, coacervate microvectors are implemented for the delivery of human bone morphogenetic protein 2 growth factor, inducing differentiation of hMSCs into osteoprogenitor cells.
33166084	7	31	theme	human	1320:1324	arg1	protein					1345:1351	human bone morphogenetic protein 2	1320:1353	human bone morphogenetic protein 2 growth factor	1320:1367	In particular, myoglobin, an oxygen storage and antioxidant protein, is successfully delivered into human mesenchymal stem cells (hMSCs) within 24 h. Furthermore, coacervate microvectors are implemented for the delivery of human bone morphogenetic protein 2 growth factor, inducing differentiation of hMSCs into osteoprogenitor cells.
33166084	8	32	theme	delivery	1501:1508	arg1	microvectors					1510:1521	delivery microvectors	1501:1521	delivery microvectors for biomedical research and the development of new therapies	1501:1582	This study demonstrates the potential of coacervate microdroplets as delivery microvectors for biomedical research and the development of new therapies.
33166084	7	33	theme	bone	1326:1329	arg1	protein					1345:1351	human bone morphogenetic protein 2	1320:1353	human bone morphogenetic protein 2 growth factor	1320:1367	In particular, myoglobin, an oxygen storage and antioxidant protein, is successfully delivered into human mesenchymal stem cells (hMSCs) within 24 h. Furthermore, coacervate microvectors are implemented for the delivery of human bone morphogenetic protein 2 growth factor, inducing differentiation of hMSCs into osteoprogenitor cells.
33166084	7	34	theme	human	1197:1201	arg1	hMSCs					1227:1231	hMSCs	1227:1231	hMSCs	1227:1231	In particular, myoglobin, an oxygen storage and antioxidant protein, is successfully delivered into human mesenchymal stem cells (hMSCs) within 24 h. Furthermore, coacervate microvectors are implemented for the delivery of human bone morphogenetic protein 2 growth factor, inducing differentiation of hMSCs into osteoprogenitor cells.
33166084	7	34	theme	human	1197:1201	arg1	cells					1220:1224	human mesenchymal stem cells	1197:1224	human mesenchymal stem cells (hMSCs)	1197:1232	In particular, myoglobin, an oxygen storage and antioxidant protein, is successfully delivered into human mesenchymal stem cells (hMSCs) within 24 h. Furthermore, coacervate microvectors are implemented for the delivery of human bone morphogenetic protein 2 growth factor, inducing differentiation of hMSCs into osteoprogenitor cells.
33166084	4	35	theme	molecules	559:567	arg1	stabilization					531:543	the stabilization	527:543	the stabilization of biological molecules, such as proteins and nucleic acids	527:603	Coacervate microdroplets are developed as cell-mimetic materials with established potential for the stabilization of biological molecules, such as proteins and nucleic acids.
33166084	4	36	theme	cell-mimetic	473:484	arg1	materials					486:494	cell-mimetic materials	473:494	cell-mimetic materials with established potential for the stabilization of biological molecules, such as proteins and nucleic acids	473:603	Coacervate microdroplets are developed as cell-mimetic materials with established potential for the stabilization of biological molecules, such as proteins and nucleic acids.
33166084	4	36	theme	cell-mimetic	473:484	arg1	microdroplets					442:454	Coacervate microdroplets	431:454	Coacervate microdroplets	431:454	Coacervate microdroplets are developed as cell-mimetic materials with established potential for the stabilization of biological molecules, such as proteins and nucleic acids.
33166084	6	37	theme	plasma	1080:1085	arg1	membrane					1087:1094	the plasma membrane	1076:1094	the plasma membrane	1076:1094	Amylose-based coacervate microdroplets are stable under physiological conditions (e.g., temperature and ionic strength), are noncytotoxic owing to their biopolymeric structure, spontaneously interacted with the cell membrane, and are able to deliver and release proteinaceous payloads beyond the plasma membrane.
33166084	3	38	theme	delivery	389:396	arg1	profile					398:404	delivery profile	389:404	delivery profile	389:404	Encapsulating biologics in a protective delivery vector has the potential to improve delivery profile and enhance performance.
33166084	4	39	theme	biological	548:557	arg1	proteins					578:585	proteins	578:585	proteins	578:585	Coacervate microdroplets are developed as cell-mimetic materials with established potential for the stabilization of biological molecules, such as proteins and nucleic acids.
33166084	4	39	theme	biological	548:557	arg1	acids					599:603	nucleic acids	591:603	nucleic acids	591:603	Coacervate microdroplets are developed as cell-mimetic materials with established potential for the stabilization of biological molecules, such as proteins and nucleic acids.
33166084	4	39	theme	biological	548:557	arg1	molecules					559:567	biological molecules	548:567	biological molecules	548:567	Coacervate microdroplets are developed as cell-mimetic materials with established potential for the stabilization of biological molecules, such as proteins and nucleic acids.
33166084	0	40	theme	Proteins	68:75	arg1	Delivery					45:52	the Delivery	41:52	the Delivery of Functional Proteins to Cells	41:84	Biopolymeric Coacervate Microvectors for the Delivery of Functional Proteins to Cells.
33166084	7	41	theme	osteoprogenitor	1409:1423	arg1	cells					1425:1429	osteoprogenitor cells	1409:1429	osteoprogenitor cells	1409:1429	In particular, myoglobin, an oxygen storage and antioxidant protein, is successfully delivered into human mesenchymal stem cells (hMSCs) within 24 h. Furthermore, coacervate microvectors are implemented for the delivery of human bone morphogenetic protein 2 growth factor, inducing differentiation of hMSCs into osteoprogenitor cells.
33166084	6	42	theme	coacervate	798:807	arg1	microdroplets					809:821	Amylose-based coacervate microdroplets	784:821	Amylose-based coacervate microdroplets	784:821	Amylose-based coacervate microdroplets are stable under physiological conditions (e.g., temperature and ionic strength), are noncytotoxic owing to their biopolymeric structure, spontaneously interacted with the cell membrane, and are able to deliver and release proteinaceous payloads beyond the plasma membrane.
33166084	1	43	theme	biologic	107:114	arg1	payloads					116:123	biologic payloads	107:123	biologic payloads	107:123	The extent to which biologic payloads can be effectively delivered to cells is a limiting factor in the development of new therapies.
33166084	5	44	dep	microvectors	656:667	arg1	comprising					670:679	comprising	670:679	comprising synthetically modified amylose polymers	670:719	Here, the development of biodegradable coacervate microvectors (comprising synthetically modified amylose polymers) is presented, for the delivery of biologic payloads to cells.
33166084	6	45	theme	ionic	888:892	arg1	strength					894:901	ionic strength	888:901	ionic strength	888:901	Amylose-based coacervate microdroplets are stable under physiological conditions (e.g., temperature and ionic strength), are noncytotoxic owing to their biopolymeric structure, spontaneously interacted with the cell membrane, and are able to deliver and release proteinaceous payloads beyond the plasma membrane.
33166084	3	46	contain	has	360:362	arg1	biologics					318:326	Encapsulating biologics	304:326	Encapsulating biologics in a protective delivery vector	304:358	Encapsulating biologics in a protective delivery vector has the potential to improve delivery profile and enhance performance.
33166084	3	46	contain	has	360:362	arg2	potential					368:376	the potential to improve delivery profile and enhance performance	364:428	the potential to improve delivery profile and enhance performance	364:428	Encapsulating biologics in a protective delivery vector has the potential to improve delivery profile and enhance performance.
33166084	7	47	theme	antioxidant	1145:1155	arg1	protein					1157:1163	an oxygen storage and antioxidant protein	1123:1163	protein	1157:1163	In particular, myoglobin, an oxygen storage and antioxidant protein, is successfully delivered into human mesenchymal stem cells (hMSCs) within 24 h. Furthermore, coacervate microvectors are implemented for the delivery of human bone morphogenetic protein 2 growth factor, inducing differentiation of hMSCs into osteoprogenitor cells.
33166084	7	47	theme	antioxidant	1145:1155	arg1	myoglobin					1112:1120	myoglobin	1112:1120	myoglobin	1112:1120	In particular, myoglobin, an oxygen storage and antioxidant protein, is successfully delivered into human mesenchymal stem cells (hMSCs) within 24 h. Furthermore, coacervate microvectors are implemented for the delivery of human bone morphogenetic protein 2 growth factor, inducing differentiation of hMSCs into osteoprogenitor cells.
33166084	8	48	theme	microdroplets	1484:1496	arg1	potential					1460:1468	the potential	1456:1468	the potential of coacervate microdroplets as delivery microvectors for biomedical research and the development of new therapies	1456:1582	This study demonstrates the potential of coacervate microdroplets as delivery microvectors for biomedical research and the development of new therapies.
33166084	3	49	from	biologics	318:326	arg1	vector					353:358	a protective delivery vector	331:358	a protective delivery vector	331:358	Encapsulating biologics in a protective delivery vector has the potential to improve delivery profile and enhance performance.
33166084	7	50	theme	hMSCs	1398:1402	arg1	differentiation					1379:1393	differentiation	1379:1393	differentiation of hMSCs into osteoprogenitor cells	1379:1429	In particular, myoglobin, an oxygen storage and antioxidant protein, is successfully delivered into human mesenchymal stem cells (hMSCs) within 24 h. Furthermore, coacervate microvectors are implemented for the delivery of human bone morphogenetic protein 2 growth factor, inducing differentiation of hMSCs into osteoprogenitor cells.
33166084	1	51	theme	new	206:208	arg1	therapies					210:218	new therapies	206:218	new therapies	206:218	The extent to which biologic payloads can be effectively delivered to cells is a limiting factor in the development of new therapies.
33166084	1	52	theme	therapies	210:218	arg1	development					191:201	the development	187:201	the development of new therapies	187:218	The extent to which biologic payloads can be effectively delivered to cells is a limiting factor in the development of new therapies.
33166084	8	53	theme	biomedical	1527:1536	arg1	research					1538:1545	biomedical research	1527:1545	biomedical research	1527:1545	This study demonstrates the potential of coacervate microdroplets as delivery microvectors for biomedical research and the development of new therapies.
33166084	4	54	theme	Coacervate	431:440	arg1	materials					486:494	cell-mimetic materials	473:494	cell-mimetic materials with established potential for the stabilization of biological molecules, such as proteins and nucleic acids	473:603	Coacervate microdroplets are developed as cell-mimetic materials with established potential for the stabilization of biological molecules, such as proteins and nucleic acids.
33166084	4	54	theme	Coacervate	431:440	arg1	microdroplets					442:454	Coacervate microdroplets	431:454	Coacervate microdroplets	431:454	Coacervate microdroplets are developed as cell-mimetic materials with established potential for the stabilization of biological molecules, such as proteins and nucleic acids.
33166084	1	55	from	factor	177:182	arg1	development					191:201	the development	187:201	the development of new therapies	187:218	The extent to which biologic payloads can be effectively delivered to cells is a limiting factor in the development of new therapies.
33166084	7	56	theme	coacervate	1260:1269	arg1	microvectors					1271:1282	coacervate microvectors	1260:1282	coacervate microvectors	1260:1282	In particular, myoglobin, an oxygen storage and antioxidant protein, is successfully delivered into human mesenchymal stem cells (hMSCs) within 24 h. Furthermore, coacervate microvectors are implemented for the delivery of human bone morphogenetic protein 2 growth factor, inducing differentiation of hMSCs into osteoprogenitor cells.
33166084	0	57	theme	Functional	57:66	arg1	Proteins					68:75	Functional Proteins	57:75	Functional Proteins	57:75	Biopolymeric Coacervate Microvectors for the Delivery of Functional Proteins to Cells.
33166084	8	58	theme	new	1570:1572	arg1	therapies					1574:1582	new therapies	1570:1582	new therapies	1570:1582	This study demonstrates the potential of coacervate microdroplets as delivery microvectors for biomedical research and the development of new therapies.
33166084	6	59	theme	proteinaceous	1046:1058	arg1	payloads					1060:1067	proteinaceous payloads	1046:1067	proteinaceous payloads	1046:1067	Amylose-based coacervate microdroplets are stable under physiological conditions (e.g., temperature and ionic strength), are noncytotoxic owing to their biopolymeric structure, spontaneously interacted with the cell membrane, and are able to deliver and release proteinaceous payloads beyond the plasma membrane.
33166084	6	60	theme	cell	995:998	arg1	membrane					1000:1007	the cell membrane	991:1007	the cell membrane	991:1007	Amylose-based coacervate microdroplets are stable under physiological conditions (e.g., temperature and ionic strength), are noncytotoxic owing to their biopolymeric structure, spontaneously interacted with the cell membrane, and are able to deliver and release proteinaceous payloads beyond the plasma membrane.
34052273	8	0	theme	bone	1124:1127	arg1	formation					1129:1137	new bone formation	1120:1137	new bone formation	1120:1137	Histological staining showed that collagen promoted collagen deposition and new bone formation.
34052273	7	1	theme	CHCS	957:960	arg1	strength					945:952	the compressive strength	929:952	the compressive strength of CHCS	929:960	The results suggested that HAP not only enhanced the compressive strength of CHCS, but also promoted the formation of calcium nodules due to its bone conductivity.
34052273	8	2	theme	new	1120:1122	arg1	formation					1129:1137	new bone formation	1120:1137	new bone formation	1120:1137	Histological staining showed that collagen promoted collagen deposition and new bone formation.
34052273	4	3	theme	chitin-hydroxyapatite	492:512	arg1	agent					612:616	crosslinking agent	599:616	crosslinking agent	599:616	The chitin-hydroxyapatite (HAP)-collagen composite scaffolds (CHCS) was prepared with epichlorohydrin (ECH) as crosslinking agent.
34052273	4	3	theme	chitin-hydroxyapatite	492:512	arg1	scaffolds					539:547	The chitin-hydroxyapatite (HAP)-collagen composite scaffolds	488:547	The chitin-hydroxyapatite (HAP)-collagen composite scaffolds (CHCS)	488:554	The chitin-hydroxyapatite (HAP)-collagen composite scaffolds (CHCS) was prepared with epichlorohydrin (ECH) as crosslinking agent.
34052273	4	3	theme	chitin-hydroxyapatite	492:512	arg1	CHCS					550:553	CHCS	550:553	CHCS	550:553	The chitin-hydroxyapatite (HAP)-collagen composite scaffolds (CHCS) was prepared with epichlorohydrin (ECH) as crosslinking agent.
34052273	7	4	theme	compressive	933:943	arg1	strength					945:952	the compressive strength	929:952	the compressive strength of CHCS	929:960	The results suggested that HAP not only enhanced the compressive strength of CHCS, but also promoted the formation of calcium nodules due to its bone conductivity.
34052273	2	5	theme	cells	341:345	arg1	problems					297:304	the two key problems	285:304	the two key problems	285:304	Biomaterials have a wide range of application prospects in bone tissue repair, and the two key problems are the selection of materials and cells.
34052273	2	5	theme	cells	341:345	arg1	selection					314:322	the selection	310:322	the selection of materials and cells	310:345	Biomaterials have a wide range of application prospects in bone tissue repair, and the two key problems are the selection of materials and cells.
34052273	6	6	dep	biocompatibility	745:760	arg1	The					741:743	The	741:743	The	741:743	The biocompatibility and osteogenic differentiation of CHCS in vitro were detected, and the effect of defect repair in vivo was evaluated.
34052273	6	7	theme	CHCS	796:799	arg1	differentiation					777:791	osteogenic differentiation	766:791	osteogenic differentiation	766:791	The biocompatibility and osteogenic differentiation of CHCS in vitro were detected, and the effect of defect repair in vivo was evaluated.
34052273	6	7	theme	CHCS	796:799	arg1	biocompatibility					745:760	biocompatibility	745:760	biocompatibility	745:760	The biocompatibility and osteogenic differentiation of CHCS in vitro were detected, and the effect of defect repair in vivo was evaluated.
34052273	2	8	from	range	227:231	arg1	repair					273:278	bone tissue repair	261:278	bone tissue repair	261:278	Biomaterials have a wide range of application prospects in bone tissue repair, and the two key problems are the selection of materials and cells.
34052273	3	9	theme	bone	422:425	arg1	materials					436:444	bone scaffold materials	422:444	bone scaffold materials	422:444	The object of this study was to discuss the structural characteristics of bone scaffold materials and their effects on bone repair in vivo.
34052273	2	10	from	prospects	248:256	arg1	repair					273:278	bone tissue repair	261:278	bone tissue repair	261:278	Biomaterials have a wide range of application prospects in bone tissue repair, and the two key problems are the selection of materials and cells.
34052273	3	11	theme	scaffold	427:434	arg1	materials					436:444	bone scaffold materials	422:444	bone scaffold materials	422:444	The object of this study was to discuss the structural characteristics of bone scaffold materials and their effects on bone repair in vivo.
34052273	3	12	theme	study	367:371	arg1	object					352:357	The object	348:357	The object of this study	348:371	The object of this study was to discuss the structural characteristics of bone scaffold materials and their effects on bone repair in vivo.
34052273	10	13	theme	bone	1260:1263	arg1	regeneration					1265:1276	bone regeneration	1260:1276	bone regeneration	1260:1276	Therefore, CHCs has immense potential in bone regeneration.
34052273	0	14	theme	Chitin-hydroxyapatite-collagen	0:29	arg1	composite					31:39	Chitin-hydroxyapatite-collagen composite	0:39	Chitin-hydroxyapatite-collagen composite	0:39	Chitin-hydroxyapatite-collagen composite scaffolds for bone regeneration.
34052273	6	15	theme	osteogenic	766:775	arg1	differentiation					777:791	osteogenic differentiation	766:791	osteogenic differentiation	766:791	The biocompatibility and osteogenic differentiation of CHCS in vitro were detected, and the effect of defect repair in vivo was evaluated.
34052273	10	16	contain	has	1235:1237	arg2	potential					1247:1255	immense potential	1239:1255	immense potential	1239:1255	Therefore, CHCs has immense potential in bone regeneration.
34052273	10	16	contain	has	1235:1237	arg1	CHCs					1230:1233	CHCs	1230:1233	CHCs	1230:1233	Therefore, CHCs has immense potential in bone regeneration.
34052273	4	17	theme	composite	529:537	arg1	agent					612:616	crosslinking agent	599:616	crosslinking agent	599:616	The chitin-hydroxyapatite (HAP)-collagen composite scaffolds (CHCS) was prepared with epichlorohydrin (ECH) as crosslinking agent.
34052273	4	17	theme	composite	529:537	arg1	scaffolds					539:547	The chitin-hydroxyapatite (HAP)-collagen composite scaffolds	488:547	The chitin-hydroxyapatite (HAP)-collagen composite scaffolds (CHCS)	488:554	The chitin-hydroxyapatite (HAP)-collagen composite scaffolds (CHCS) was prepared with epichlorohydrin (ECH) as crosslinking agent.
34052273	4	17	theme	composite	529:537	arg1	CHCS					550:553	CHCS	550:553	CHCS	550:553	The chitin-hydroxyapatite (HAP)-collagen composite scaffolds (CHCS) was prepared with epichlorohydrin (ECH) as crosslinking agent.
34052273	7	18	theme	bone	1025:1028	arg1	conductivity					1030:1041	its bone conductivity	1021:1041	its bone conductivity	1021:1041	The results suggested that HAP not only enhanced the compressive strength of CHCS, but also promoted the formation of calcium nodules due to its bone conductivity.
34052273	9	19	theme	CHCS	1173:1176	arg1	transplantation					1178:1192	CHCS transplantation	1173:1192	CHCS transplantation	1173:1192	X-ray images also indicated that CHCS transplantation accelerated bone repair.
34052273	4	20	theme	crosslinking	599:610	arg1	agent					612:616	crosslinking agent	599:616	crosslinking agent	599:616	The chitin-hydroxyapatite (HAP)-collagen composite scaffolds (CHCS) was prepared with epichlorohydrin (ECH) as crosslinking agent.
34052273	4	20	theme	crosslinking	599:610	arg1	scaffolds					539:547	The chitin-hydroxyapatite (HAP)-collagen composite scaffolds	488:547	The chitin-hydroxyapatite (HAP)-collagen composite scaffolds (CHCS)	488:554	The chitin-hydroxyapatite (HAP)-collagen composite scaffolds (CHCS) was prepared with epichlorohydrin (ECH) as crosslinking agent.
34052273	9	21	theme	bone	1206:1209	arg1	repair					1211:1216	bone repair	1206:1216	bone repair	1206:1216	X-ray images also indicated that CHCS transplantation accelerated bone repair.
34052273	6	22	theme	repair	850:855	arg1	effect					833:838	the effect	829:838	the effect of defect repair in vivo	829:863	The biocompatibility and osteogenic differentiation of CHCS in vitro were detected, and the effect of defect repair in vivo was evaluated.
34052273	2	23	theme	key	293:295	arg1	problems					297:304	the two key problems	285:304	the two key problems	285:304	Biomaterials have a wide range of application prospects in bone tissue repair, and the two key problems are the selection of materials and cells.
34052273	2	23	theme	key	293:295	arg1	selection					314:322	the selection	310:322	the selection of materials and cells	310:345	Biomaterials have a wide range of application prospects in bone tissue repair, and the two key problems are the selection of materials and cells.
34052273	2	24	from	repair	273:278	arg1	range					227:231	a wide range	220:231	a wide range of application prospects in bone tissue repair	220:278	Biomaterials have a wide range of application prospects in bone tissue repair, and the two key problems are the selection of materials and cells.
34052273	2	25	contain	have	215:218	arg2	range					227:231	a wide range	220:231	a wide range of application prospects in bone tissue repair	220:278	Biomaterials have a wide range of application prospects in bone tissue repair, and the two key problems are the selection of materials and cells.
34052273	2	25	contain	have	215:218	arg1	Biomaterials					202:213	Biomaterials	202:213	Biomaterials	202:213	Biomaterials have a wide range of application prospects in bone tissue repair, and the two key problems are the selection of materials and cells.
34052273	2	26	theme	materials	327:335	arg1	problems					297:304	the two key problems	285:304	the two key problems	285:304	Biomaterials have a wide range of application prospects in bone tissue repair, and the two key problems are the selection of materials and cells.
34052273	2	26	theme	materials	327:335	arg1	selection					314:322	the selection	310:322	the selection of materials and cells	310:345	Biomaterials have a wide range of application prospects in bone tissue repair, and the two key problems are the selection of materials and cells.
34052273	6	27	theme	defect	843:848	arg1	repair					850:855	defect repair	843:855	defect repair	843:855	The biocompatibility and osteogenic differentiation of CHCS in vitro were detected, and the effect of defect repair in vivo was evaluated.
34052273	2	28	theme	prospects	248:256	arg1	range					227:231	a wide range	220:231	a wide range of application prospects in bone tissue repair	220:278	Biomaterials have a wide range of application prospects in bone tissue repair, and the two key problems are the selection of materials and cells.
34052273	3	29	theme	materials	436:444	arg1	characteristics					403:417	the structural characteristics	388:417	the structural characteristics of bone scaffold materials	388:444	The object of this study was to discuss the structural characteristics of bone scaffold materials and their effects on bone repair in vivo.
34052273	3	29	theme	materials	436:444	arg1	effects					456:462	their effects	450:462	their effects on bone repair in vivo	450:485	The object of this study was to discuss the structural characteristics of bone scaffold materials and their effects on bone repair in vivo.
34052273	0	30	theme	bone	55:58	arg1	regeneration					60:71	bone regeneration	55:71	bone regeneration	55:71	Chitin-hydroxyapatite-collagen composite scaffolds for bone regeneration.
34052273	4	31	theme	HAP	515:517	arg1	agent					612:616	crosslinking agent	599:616	crosslinking agent	599:616	The chitin-hydroxyapatite (HAP)-collagen composite scaffolds (CHCS) was prepared with epichlorohydrin (ECH) as crosslinking agent.
34052273	4	31	theme	HAP	515:517	arg1	scaffolds					539:547	The chitin-hydroxyapatite (HAP)-collagen composite scaffolds	488:547	The chitin-hydroxyapatite (HAP)-collagen composite scaffolds (CHCS)	488:554	The chitin-hydroxyapatite (HAP)-collagen composite scaffolds (CHCS) was prepared with epichlorohydrin (ECH) as crosslinking agent.
34052273	4	31	theme	HAP	515:517	arg1	CHCS					550:553	CHCS	550:553	CHCS	550:553	The chitin-hydroxyapatite (HAP)-collagen composite scaffolds (CHCS) was prepared with epichlorohydrin (ECH) as crosslinking agent.
34052273	3	32	theme	structural	392:401	arg1	characteristics					403:417	the structural characteristics	388:417	the structural characteristics of bone scaffold materials	388:444	The object of this study was to discuss the structural characteristics of bone scaffold materials and their effects on bone repair in vivo.
34052273	2	33	theme	application	236:246	arg1	prospects					248:256	application prospects	236:256	application prospects in bone tissue repair	236:278	Biomaterials have a wide range of application prospects in bone tissue repair, and the two key problems are the selection of materials and cells.
34052273	7	34	theme	nodules	1006:1012	arg1	formation					985:993	the formation	981:993	the formation of calcium nodules due to its bone conductivity	981:1041	The results suggested that HAP not only enhanced the compressive strength of CHCS, but also promoted the formation of calcium nodules due to its bone conductivity.
34052273	8	35	theme	collagen	1096:1103	arg1	deposition					1105:1114	collagen deposition	1096:1114	collagen deposition	1096:1114	Histological staining showed that collagen promoted collagen deposition and new bone formation.
34052273	5	36	theme	water	691:695	arg1	absorbency					697:706	water absorbency	691:706	water absorbency	691:706	The structure was characterized and the compressive strength, porosity, water absorbency and stability were investigated.
34052273	4	37	theme	-collagen	519:527	arg1	agent					612:616	crosslinking agent	599:616	crosslinking agent	599:616	The chitin-hydroxyapatite (HAP)-collagen composite scaffolds (CHCS) was prepared with epichlorohydrin (ECH) as crosslinking agent.
34052273	4	37	theme	-collagen	519:527	arg1	scaffolds					539:547	The chitin-hydroxyapatite (HAP)-collagen composite scaffolds	488:547	The chitin-hydroxyapatite (HAP)-collagen composite scaffolds (CHCS)	488:554	The chitin-hydroxyapatite (HAP)-collagen composite scaffolds (CHCS) was prepared with epichlorohydrin (ECH) as crosslinking agent.
34052273	4	37	theme	-collagen	519:527	arg1	CHCS					550:553	CHCS	550:553	CHCS	550:553	The chitin-hydroxyapatite (HAP)-collagen composite scaffolds (CHCS) was prepared with epichlorohydrin (ECH) as crosslinking agent.
34052273	8	38	theme	Histological	1044:1055	arg1	staining					1057:1064	Histological staining	1044:1064	Histological staining	1044:1064	Histological staining showed that collagen promoted collagen deposition and new bone formation.
34052273	2	39	theme	wide	222:225	arg1	range					227:231	a wide range	220:231	a wide range of application prospects in bone tissue repair	220:278	Biomaterials have a wide range of application prospects in bone tissue repair, and the two key problems are the selection of materials and cells.
34052273	1	40	theme	bone	131:134	arg1	method					194:199	an effective method	181:199	an effective method	181:199	Bone defect is usually difficult to recover quickly, and bone scaffold transplantation is considered to be an effective method.
34052273	1	40	theme	bone	131:134	arg1	transplantation					145:159	bone scaffold transplantation	131:159	bone scaffold transplantation	131:159	Bone defect is usually difficult to recover quickly, and bone scaffold transplantation is considered to be an effective method.
34052273	5	41	theme	compressive	659:669	arg1	strength					671:678	the compressive strength	655:678	the compressive strength	655:678	The structure was characterized and the compressive strength, porosity, water absorbency and stability were investigated.
34052273	3	42	from	effects	456:462	arg1	repair					472:477	bone repair	467:477	bone repair in vivo	467:485	The object of this study was to discuss the structural characteristics of bone scaffold materials and their effects on bone repair in vivo.
34052273	1	43	theme	scaffold	136:143	arg1	method					194:199	an effective method	181:199	an effective method	181:199	Bone defect is usually difficult to recover quickly, and bone scaffold transplantation is considered to be an effective method.
34052273	1	43	theme	scaffold	136:143	arg1	transplantation					145:159	bone scaffold transplantation	131:159	bone scaffold transplantation	131:159	Bone defect is usually difficult to recover quickly, and bone scaffold transplantation is considered to be an effective method.
34052273	10	44	theme	immense	1239:1245	arg1	potential					1247:1255	immense potential	1239:1255	immense potential	1239:1255	Therefore, CHCs has immense potential in bone regeneration.
34052273	7	45	theme	calcium	998:1004	arg1	nodules					1006:1012	calcium nodules	998:1012	calcium nodules	998:1012	The results suggested that HAP not only enhanced the compressive strength of CHCS, but also promoted the formation of calcium nodules due to its bone conductivity.
34052273	2	46	theme	tissue	266:271	arg1	repair					273:278	bone tissue repair	261:278	bone tissue repair	261:278	Biomaterials have a wide range of application prospects in bone tissue repair, and the two key problems are the selection of materials and cells.
34052273	3	47	from	characteristics	403:417	arg1	repair					472:477	bone repair	467:477	bone repair in vivo	467:485	The object of this study was to discuss the structural characteristics of bone scaffold materials and their effects on bone repair in vivo.
34052273	3	48	theme	bone	467:470	arg1	repair					472:477	bone repair	467:477	bone repair in vivo	467:485	The object of this study was to discuss the structural characteristics of bone scaffold materials and their effects on bone repair in vivo.
34052273	2	49	theme	bone	261:264	arg1	repair					273:278	bone tissue repair	261:278	bone tissue repair	261:278	Biomaterials have a wide range of application prospects in bone tissue repair, and the two key problems are the selection of materials and cells.
34052273	1	50	theme	effective	184:192	arg1	method					194:199	an effective method	181:199	an effective method	181:199	Bone defect is usually difficult to recover quickly, and bone scaffold transplantation is considered to be an effective method.
34052273	1	50	theme	effective	184:192	arg1	transplantation					145:159	bone scaffold transplantation	131:159	bone scaffold transplantation	131:159	Bone defect is usually difficult to recover quickly, and bone scaffold transplantation is considered to be an effective method.
34052273	1	51	theme	Bone	74:77	arg1	defect					79:84	Bone defect	74:84	Bone defect	74:84	Bone defect is usually difficult to recover quickly, and bone scaffold transplantation is considered to be an effective method.
34052273	9	52	theme	X-ray	1140:1144	arg1	images					1146:1151	X-ray images	1140:1151	X-ray images	1140:1151	X-ray images also indicated that CHCS transplantation accelerated bone repair.
33319575	6	0	theme	logistic	904:911	arg1	model					924:928	A multinomial logistic regression model	890:928	A multinomial logistic regression model	890:928	A multinomial logistic regression model was performed to explain body composition according to consumption of food groups.
33319575	11	1	theme	muscle	1884:1889	arg1	mass					1891:1894	muscle mass	1884:1894	muscle mass	1884:1894	Conclusions: the consumption of sugars seems to have a close relationship with variables of body composition such as visceral fat, bone mass, and muscle mass.
33319575	11	2	with	relationship	1799:1810	arg1	variables					1817:1825	variables	1817:1825	variables of body composition such as visceral fat, bone mass, and muscle mass	1817:1894	Conclusions: the consumption of sugars seems to have a close relationship with variables of body composition such as visceral fat, bone mass, and muscle mass.
33319575	11	2	with	relationship	1799:1810	arg1	mass					1891:1894	muscle mass	1884:1894	muscle mass	1884:1894	Conclusions: the consumption of sugars seems to have a close relationship with variables of body composition such as visceral fat, bone mass, and muscle mass.
33319575	11	2	with	relationship	1799:1810	arg1	fat					1864:1866	visceral fat	1855:1866	visceral fat	1855:1866	Conclusions: the consumption of sugars seems to have a close relationship with variables of body composition such as visceral fat, bone mass, and muscle mass.
33319575	11	2	with	relationship	1799:1810	arg1	mass					1874:1877	bone mass	1869:1877	bone mass	1869:1877	Conclusions: the consumption of sugars seems to have a close relationship with variables of body composition such as visceral fat, bone mass, and muscle mass.
33319575	9	3	theme	grains	1524:1529	arg1	Consumption					1482:1492	Consumption	1482:1492	Consumption of eggs, meats, sausages, and grains	1482:1529	Consumption of eggs, meats, sausages, and grains favors an acceptable percentage of visceral fat (OR = 0.14).
33319575	10	4	dep	percentage	1669:1678	arg1	=					1693:1693	=	1693:1693	=	1693:1693	The consumption of fruits and vegetables increases the risk of having a high percentage of water (OR = 5.41), and low fat percentage (OR = 3.60).
33319575	1	5	theme	social	168:173	arg1	dynamics					175:182	a social dynamics	166:182	a social dynamics that has generated complex lifestyles and, especially, an imbalance in the intake of macro and micronutrients, characterized by having a high caloric density intake, which results in significant excess of weight	166:394	Introduction: young university students face a social dynamics that has generated complex lifestyles and, especially, an imbalance in the intake of macro and micronutrients, characterized by having a high caloric density intake, which results in significant excess of weight.
33319575	7	6	theme	body	1104:1107	arg1	composition					1109:1119	body composition	1104:1119	body composition	1104:1119	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	3	7	theme	consumption	548:558	arg1	pattern					532:538	pattern	532:538	pattern of food consumption and body composition in university students	532:602	This research aimed to determine the relationship between pattern of food consumption and body composition in university students.
33319575	5	8	theme	reminder	859:866	arg1	method					868:873	a 24-hour reminder method	849:873	a 24-hour reminder method	849:873	Sociodemographic variables obtained by a survey, body composition variables obtained by bioimpedance, and food groups and their categories obtained via a 24-hour reminder method were included.
33319575	9	9	theme	visceral	1566:1573	arg1	fat					1575:1577	visceral fat	1566:1577	visceral fat (OR = 0.14)	1566:1589	Consumption of eggs, meats, sausages, and grains favors an acceptable percentage of visceral fat (OR = 0.14).
33319575	11	10	theme	composition	1835:1845	arg1	variables					1817:1825	variables	1817:1825	variables of body composition such as visceral fat, bone mass, and muscle mass	1817:1894	Conclusions: the consumption of sugars seems to have a close relationship with variables of body composition such as visceral fat, bone mass, and muscle mass.
33319575	11	10	theme	composition	1835:1845	arg1	mass					1891:1894	muscle mass	1884:1894	muscle mass	1884:1894	Conclusions: the consumption of sugars seems to have a close relationship with variables of body composition such as visceral fat, bone mass, and muscle mass.
33319575	11	10	theme	composition	1835:1845	arg1	fat					1864:1866	visceral fat	1855:1866	visceral fat	1855:1866	Conclusions: the consumption of sugars seems to have a close relationship with variables of body composition such as visceral fat, bone mass, and muscle mass.
33319575	11	10	theme	composition	1835:1845	arg1	mass					1874:1877	bone mass	1869:1877	bone mass	1869:1877	Conclusions: the consumption of sugars seems to have a close relationship with variables of body composition such as visceral fat, bone mass, and muscle mass.
33319575	8	11	theme	visceral	1374:1381	arg1	2.71					1393:1396	= 2.71	1391:1396	= 2.71	1391:1396	The multiple regression model showed that sugar consumption increases the risk of having visceral fat (OR = 2.71), and increases the probability of being classified as an obese person (OR = 2.55).
33319575	8	11	theme	visceral	1374:1381	arg1	fat					1383:1385	visceral fat	1374:1385	visceral fat (OR = 2.71)	1374:1397	The multiple regression model showed that sugar consumption increases the risk of having visceral fat (OR = 2.71), and increases the probability of being classified as an obese person (OR = 2.55).
33319575	5	12	theme	Sociodemographic	697:712	arg1	variables					714:722	Sociodemographic variables	697:722	Sociodemographic variables obtained by a survey	697:743	Sociodemographic variables obtained by a survey, body composition variables obtained by bioimpedance, and food groups and their categories obtained via a 24-hour reminder method were included.
33319575	8	13	dep	=	1473:1473	arg1	2.55					1475:1478	2.55	1475:1478	2.55	1475:1478	The multiple regression model showed that sugar consumption increases the risk of having visceral fat (OR = 2.71), and increases the probability of being classified as an obese person (OR = 2.55).
33319575	11	14	theme	bone	1869:1872	arg1	mass					1874:1877	bone mass	1869:1877	bone mass	1869:1877	Conclusions: the consumption of sugars seems to have a close relationship with variables of body composition such as visceral fat, bone mass, and muscle mass.
33319575	4	15	theme	students	687:694	arg1	sample					673:678	a sample	671:678	a sample of 189 students	671:694	Method: an observational, descriptive, cross-sectional study with a sample of 189 students.
33319575	4	16	dep	Method	605:610	arg1	study					660:664	an observational, descriptive, cross-sectional study	613:664	study	660:664	Method: an observational, descriptive, cross-sectional study with a sample of 189 students.
33319575	3	17	theme	body	564:567	arg1	composition					569:579	body composition	564:579	body composition	564:579	This research aimed to determine the relationship between pattern of food consumption and body composition in university students.
33319575	8	18	theme	=	1391:1391	arg1	2.71					1393:1396	= 2.71	1391:1396	= 2.71	1391:1396	The multiple regression model showed that sugar consumption increases the risk of having visceral fat (OR = 2.71), and increases the probability of being classified as an obese person (OR = 2.55).
33319575	8	18	theme	=	1391:1391	arg1	fat					1383:1385	visceral fat	1374:1385	visceral fat (OR = 2.71)	1374:1397	The multiple regression model showed that sugar consumption increases the risk of having visceral fat (OR = 2.71), and increases the probability of being classified as an obese person (OR = 2.55).
33319575	7	19	theme	significant	1036:1046	arg1	associations					1048:1059	statistically significant associations	1022:1059	statistically significant associations	1022:1059	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	11	20	theme	close	1793:1797	arg1	relationship					1799:1810	a close relationship	1791:1810	a close relationship with variables of body composition such as visceral fat, bone mass, and muscle mass	1791:1894	Conclusions: the consumption of sugars seems to have a close relationship with variables of body composition such as visceral fat, bone mass, and muscle mass.
33319575	7	21	theme	consumption	1207:1217	arg1	fat					1134:1136	visceral fat	1125:1136	visceral fat	1125:1136	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	21	theme	consumption	1207:1217	arg1	consumption					1142:1152	consumption	1142:1152	consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013)	1142:1282	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	10	22	theme	high	1664:1667	arg1	percentage					1669:1678	a high percentage	1662:1678	a high percentage of water (OR = 5.41)	1662:1699	The consumption of fruits and vegetables increases the risk of having a high percentage of water (OR = 5.41), and low fat percentage (OR = 3.60).
33319575	1	23	theme	university	141:150	arg1	students					152:159	young university students	135:159	young university students	135:159	Introduction: young university students face a social dynamics that has generated complex lifestyles and, especially, an imbalance in the intake of macro and micronutrients, characterized by having a high caloric density intake, which results in significant excess of weight.
33319575	6	24	theme	groups	1005:1010	arg1	consumption					985:995	consumption	985:995	consumption of food groups	985:1010	A multinomial logistic regression model was performed to explain body composition according to consumption of food groups.
33319575	2	25	theme	body	456:459	arg1	composition					461:471	body composition	456:471	body composition	456:471	It is unknown if these consumption patterns are related to body composition.
33319575	0	26	from	pattern	22:28	arg1	university					74:83	university	74:83	university	74:83	[Relationship between pattern of food consumption and body composition in university students: a cross-sectional study].
33319575	13	27	theme	accurate	2229:2236	arg1	association					2238:2248	a more accurate association	2222:2248	a more accurate association	2222:2248	Studies to establish associations between body composition and food consumption should be carried out taking into account specific intakes in order to establish a more accurate association.
33319575	5	28	theme	composition	751:761	arg1	variables					763:771	body composition variables	746:771	body composition variables obtained by bioimpedance	746:796	Sociodemographic variables obtained by a survey, body composition variables obtained by bioimpedance, and food groups and their categories obtained via a 24-hour reminder method were included.
33319575	1	29	theme	high	321:324	arg1	intake					342:347	a high caloric density intake	319:347	a high caloric density intake	319:347	Introduction: young university students face a social dynamics that has generated complex lifestyles and, especially, an imbalance in the intake of macro and micronutrients, characterized by having a high caloric density intake, which results in significant excess of weight.
33319575	1	30	theme	density	334:340	arg1	intake					342:347	a high caloric density intake	319:347	a high caloric density intake	319:347	Introduction: young university students face a social dynamics that has generated complex lifestyles and, especially, an imbalance in the intake of macro and micronutrients, characterized by having a high caloric density intake, which results in significant excess of weight.
33319575	7	31	theme	p	1191:1191	arg1	sausages					1170:1177	sausages	1170:1177	sausages	1170:1177	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	31	theme	p	1191:1191	arg1	0.024					1195:1199	p = 0.024	1191:1199	p = 0.024	1191:1199	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	32	theme	=	1275:1275	arg1	0.013					1277:1281	p = 0.013	1273:1281	p = 0.013	1273:1281	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	32	theme	=	1275:1275	arg1	mass					1267:1270	muscle mass	1260:1270	muscle mass (p = 0.013)	1260:1282	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	8	33	theme	regression	1298:1307	arg1	model					1309:1313	The multiple regression model	1285:1313	The multiple regression model	1285:1313	The multiple regression model showed that sugar consumption increases the risk of having visceral fat (OR = 2.71), and increases the probability of being classified as an obese person (OR = 2.55).
33319575	10	34	contain	having	1655:1660	arg1	risk					1647:1650	the risk	1643:1650	the risk of having a high percentage of water (OR = 5.41), and low fat percentage (OR = 3.60)	1643:1735	The consumption of fruits and vegetables increases the risk of having a high percentage of water (OR = 5.41), and low fat percentage (OR = 3.60).
33319575	10	34	contain	having	1655:1660	arg2	OR					1726:1727	OR = 3.60	1726:1734	OR = 3.60	1726:1734	The consumption of fruits and vegetables increases the risk of having a high percentage of water (OR = 5.41), and low fat percentage (OR = 3.60).
33319575	10	34	contain	having	1655:1660	arg2	percentage					1669:1678	a high percentage	1662:1678	a high percentage of water (OR = 5.41)	1662:1699	The consumption of fruits and vegetables increases the risk of having a high percentage of water (OR = 5.41), and low fat percentage (OR = 3.60).
33319575	10	34	contain	having	1655:1660	arg2	percentage					1714:1723	low fat percentage	1706:1723	low fat percentage (OR = 3.60)	1706:1735	The consumption of fruits and vegetables increases the risk of having a high percentage of water (OR = 5.41), and low fat percentage (OR = 3.60).
33319575	7	35	theme	sausages	1170:1177	arg1	fat					1134:1136	visceral fat	1125:1136	visceral fat	1125:1136	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	35	theme	sausages	1170:1177	arg1	consumption					1142:1152	consumption	1142:1152	consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013)	1142:1282	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	1	36	theme	macro	269:273	arg1	intake					259:264	the intake	255:264	the intake of macro and micronutrients	255:292	Introduction: young university students face a social dynamics that has generated complex lifestyles and, especially, an imbalance in the intake of macro and micronutrients, characterized by having a high caloric density intake, which results in significant excess of weight.
33319575	13	37	theme	specific	2183:2190	arg1	intakes					2192:2198	specific intakes	2183:2198	specific intakes	2183:2198	Studies to establish associations between body composition and food consumption should be carried out taking into account specific intakes in order to establish a more accurate association.
33319575	7	38	theme	muscle	1260:1265	arg1	0.013					1277:1281	p = 0.013	1273:1281	p = 0.013	1273:1281	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	38	theme	muscle	1260:1265	arg1	mass					1267:1270	muscle mass	1260:1270	muscle mass (p = 0.013)	1260:1282	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	8	39	theme	sugar	1327:1331	arg1	consumption					1333:1343	sugar consumption	1327:1343	sugar consumption	1327:1343	The multiple regression model showed that sugar consumption increases the risk of having visceral fat (OR = 2.71), and increases the probability of being classified as an obese person (OR = 2.55).
33319575	7	40	theme	meats	1163:1167	arg1	fat					1134:1136	visceral fat	1125:1136	visceral fat	1125:1136	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	40	theme	meats	1163:1167	arg1	consumption					1142:1152	consumption	1142:1152	consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013)	1142:1282	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	1	41	theme	micronutrients	279:292	arg1	intake					259:264	the intake	255:264	the intake of macro and micronutrients	255:292	Introduction: young university students face a social dynamics that has generated complex lifestyles and, especially, an imbalance in the intake of macro and micronutrients, characterized by having a high caloric density intake, which results in significant excess of weight.
33319575	0	42	theme	food	33:36	arg1	consumption					38:48	food consumption	33:48	food consumption	33:48	[Relationship between pattern of food consumption and body composition in university students: a cross-sectional study].
33319575	4	43	dep	observational	616:628	arg1	descriptive					631:641	descriptive	631:641	descriptive	631:641	Method: an observational, descriptive, cross-sectional study with a sample of 189 students.
33319575	0	44	theme	composition	59:69	arg1	pattern					22:28	pattern	22:28	pattern of food consumption and body composition in university students	22:92	[Relationship between pattern of food consumption and body composition in university students: a cross-sectional study].
33319575	12	45	theme	low	1955:1957	arg1	percentage					1959:1968	a low percentage	1953:1968	a low percentage of fat	1953:1975	The consumption of fruits and vegetables was related to a low percentage of fat; however, their consumption is proportionally related to high levels of body water.
33319575	7	46	theme	eggs	1157:1160	arg1	fat					1134:1136	visceral fat	1125:1136	visceral fat	1125:1136	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	46	theme	eggs	1157:1160	arg1	consumption					1142:1152	consumption	1142:1152	consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013)	1142:1282	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	9	47	theme	eggs	1497:1500	arg1	Consumption					1482:1492	Consumption	1482:1492	Consumption of eggs, meats, sausages, and grains	1482:1529	Consumption of eggs, meats, sausages, and grains favors an acceptable percentage of visceral fat (OR = 0.14).
33319575	7	48	theme	=	1247:1247	arg1	p					1245:1245	p = 0.029	1245:1253	p = 0.029	1245:1253	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	48	theme	=	1247:1247	arg1	mass					1239:1242	bone mass	1234:1242	bone mass (p = 0.029)	1234:1254	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	11	49	theme	sugars	1770:1775	arg1	consumption					1755:1765	the consumption	1751:1765	the consumption of sugars	1751:1775	Conclusions: the consumption of sugars seems to have a close relationship with variables of body composition such as visceral fat, bone mass, and muscle mass.
33319575	13	50	dep	Studies	2061:2067	arg1	establish					2072:2080	establish	2072:2080	to establish associations between body composition and food consumption	2069:2139	Studies to establish associations between body composition and food consumption should be carried out taking into account specific intakes in order to establish a more accurate association.
33319575	9	51	theme	meats	1503:1507	arg1	Consumption					1482:1492	Consumption	1482:1492	Consumption of eggs, meats, sausages, and grains	1482:1529	Consumption of eggs, meats, sausages, and grains favors an acceptable percentage of visceral fat (OR = 0.14).
33319575	6	52	theme	regression	913:922	arg1	model					924:928	A multinomial logistic regression model	890:928	A multinomial logistic regression model	890:928	A multinomial logistic regression model was performed to explain body composition according to consumption of food groups.
33319575	9	53	theme	sausages	1510:1517	arg1	Consumption					1482:1492	Consumption	1482:1492	Consumption of eggs, meats, sausages, and grains	1482:1529	Consumption of eggs, meats, sausages, and grains favors an acceptable percentage of visceral fat (OR = 0.14).
33319575	7	54	theme	bone	1234:1237	arg1	p					1245:1245	p = 0.029	1245:1253	p = 0.029	1245:1253	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	54	theme	bone	1234:1237	arg1	mass					1239:1242	bone mass	1234:1242	bone mass (p = 0.029)	1234:1254	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	10	55	theme	=	1729:1729	arg1	OR					1726:1727	OR = 3.60	1726:1734	OR = 3.60	1726:1734	The consumption of fruits and vegetables increases the risk of having a high percentage of water (OR = 5.41), and low fat percentage (OR = 3.60).
33319575	10	55	theme	=	1729:1729	arg1	percentage					1714:1723	low fat percentage	1706:1723	low fat percentage (OR = 3.60)	1706:1735	The consumption of fruits and vegetables increases the risk of having a high percentage of water (OR = 5.41), and low fat percentage (OR = 3.60).
33319575	12	56	theme	high	2034:2037	arg1	levels					2039:2044	high levels	2034:2044	high levels of body water	2034:2058	The consumption of fruits and vegetables was related to a low percentage of fat; however, their consumption is proportionally related to high levels of body water.
33319575	6	57	theme	multinomial	892:902	arg1	model					924:928	A multinomial logistic regression model	890:928	A multinomial logistic regression model	890:928	A multinomial logistic regression model was performed to explain body composition according to consumption of food groups.
33319575	7	58	theme	sugars	1222:1227	arg1	0.024					1195:1199	p = 0.024	1191:1199	p = 0.024	1191:1199	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	58	theme	sugars	1222:1227	arg1	sausages					1170:1177	sausages	1170:1177	sausages	1170:1177	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	58	theme	sugars	1222:1227	arg1	grains					1183:1188	grains	1183:1188	grains	1183:1188	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	58	theme	sugars	1222:1227	arg1	eggs					1157:1160	eggs	1157:1160	eggs	1157:1160	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	58	theme	sugars	1222:1227	arg1	consumption					1207:1217	consumption	1207:1217	consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013)	1207:1282	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	58	theme	sugars	1222:1227	arg1	meats					1163:1167	meats	1163:1167	meats	1163:1167	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	10	59	theme	fat	1710:1712	arg1	OR					1726:1727	OR = 3.60	1726:1734	OR = 3.60	1726:1734	The consumption of fruits and vegetables increases the risk of having a high percentage of water (OR = 5.41), and low fat percentage (OR = 3.60).
33319575	10	59	theme	fat	1710:1712	arg1	percentage					1714:1723	low fat percentage	1706:1723	low fat percentage (OR = 3.60)	1706:1735	The consumption of fruits and vegetables increases the risk of having a high percentage of water (OR = 5.41), and low fat percentage (OR = 3.60).
33319575	7	60	theme	visceral	1125:1132	arg1	fat					1134:1136	visceral fat	1125:1136	visceral fat	1125:1136	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	10	61	theme	fruits	1611:1616	arg1	consumption					1596:1606	The consumption	1592:1606	The consumption of fruits and vegetables	1592:1631	The consumption of fruits and vegetables increases the risk of having a high percentage of water (OR = 5.41), and low fat percentage (OR = 3.60).
33319575	11	62	contain	have	1786:1789	arg1	consumption					1755:1765	the consumption	1751:1765	the consumption of sugars	1751:1775	Conclusions: the consumption of sugars seems to have a close relationship with variables of body composition such as visceral fat, bone mass, and muscle mass.
33319575	11	62	contain	have	1786:1789	arg2	relationship					1799:1810	a close relationship	1791:1810	a close relationship with variables of body composition such as visceral fat, bone mass, and muscle mass	1791:1894	Conclusions: the consumption of sugars seems to have a close relationship with variables of body composition such as visceral fat, bone mass, and muscle mass.
33319575	7	63	with	eggs	1157:1160	arg1	0.013					1277:1281	p = 0.013	1273:1281	p = 0.013	1273:1281	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	63	with	eggs	1157:1160	arg1	p					1245:1245	p = 0.029	1245:1253	p = 0.029	1245:1253	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	63	with	eggs	1157:1160	arg1	mass					1267:1270	muscle mass	1260:1270	muscle mass (p = 0.013)	1260:1282	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	63	with	eggs	1157:1160	arg1	mass					1239:1242	bone mass	1234:1242	bone mass (p = 0.029)	1234:1254	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	10	64	dep	=	1693:1693	arg1	5.41					1695:1698	5.41	1695:1698	5.41	1695:1698	The consumption of fruits and vegetables increases the risk of having a high percentage of water (OR = 5.41), and low fat percentage (OR = 3.60).
33319575	2	65	theme	consumption	420:430	arg1	patterns					432:439	these consumption patterns	414:439	these consumption patterns	414:439	It is unknown if these consumption patterns are related to body composition.
33319575	9	66	theme	acceptable	1541:1550	arg1	percentage					1552:1561	an acceptable percentage	1538:1561	an acceptable percentage of visceral fat (OR = 0.14)	1538:1589	Consumption of eggs, meats, sausages, and grains favors an acceptable percentage of visceral fat (OR = 0.14).
33319575	7	67	with	meats	1163:1167	arg1	0.013					1277:1281	p = 0.013	1273:1281	p = 0.013	1273:1281	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	67	with	meats	1163:1167	arg1	p					1245:1245	p = 0.029	1245:1253	p = 0.029	1245:1253	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	67	with	meats	1163:1167	arg1	mass					1267:1270	muscle mass	1260:1270	muscle mass (p = 0.013)	1260:1282	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	67	with	meats	1163:1167	arg1	mass					1239:1242	bone mass	1234:1242	bone mass (p = 0.029)	1234:1254	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	9	68	dep	fat	1575:1577	arg1	=					1583:1583	=	1583:1583	=	1583:1583	Consumption of eggs, meats, sausages, and grains favors an acceptable percentage of visceral fat (OR = 0.14).
33319575	3	69	theme	food	543:546	arg1	consumption					548:558	food consumption	543:558	food consumption	543:558	This research aimed to determine the relationship between pattern of food consumption and body composition in university students.
33319575	5	70	theme	24-hour	851:857	arg1	method					868:873	a 24-hour reminder method	849:873	a 24-hour reminder method	849:873	Sociodemographic variables obtained by a survey, body composition variables obtained by bioimpedance, and food groups and their categories obtained via a 24-hour reminder method were included.
33319575	7	71	with	sausages	1170:1177	arg1	0.013					1277:1281	p = 0.013	1273:1281	p = 0.013	1273:1281	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	71	with	sausages	1170:1177	arg1	p					1245:1245	p = 0.029	1245:1253	p = 0.029	1245:1253	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	71	with	sausages	1170:1177	arg1	mass					1267:1270	muscle mass	1260:1270	muscle mass (p = 0.013)	1260:1282	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	71	with	sausages	1170:1177	arg1	mass					1239:1242	bone mass	1234:1242	bone mass (p = 0.029)	1234:1254	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	4	72	theme	cross-sectional	644:658	arg1	study					660:664	an observational, descriptive, cross-sectional study	613:664	study	660:664	Method: an observational, descriptive, cross-sectional study with a sample of 189 students.
33319575	7	73	theme	food	1083:1086	arg1	consumption					1088:1098	food consumption	1083:1098	food consumption	1083:1098	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	10	74	theme	vegetables	1622:1631	arg1	consumption					1596:1606	The consumption	1592:1606	The consumption of fruits and vegetables	1592:1631	The consumption of fruits and vegetables increases the risk of having a high percentage of water (OR = 5.41), and low fat percentage (OR = 3.60).
33319575	11	75	theme	body	1830:1833	arg1	composition					1835:1845	body composition	1830:1845	body composition	1830:1845	Conclusions: the consumption of sugars seems to have a close relationship with variables of body composition such as visceral fat, bone mass, and muscle mass.
33319575	6	76	theme	body	955:958	arg1	composition					960:970	body composition	955:970	body composition according to consumption of food groups	955:1010	A multinomial logistic regression model was performed to explain body composition according to consumption of food groups.
33319575	9	77	theme	fat	1575:1577	arg1	percentage					1552:1561	an acceptable percentage	1538:1561	an acceptable percentage of visceral fat (OR = 0.14)	1538:1589	Consumption of eggs, meats, sausages, and grains favors an acceptable percentage of visceral fat (OR = 0.14).
33319575	7	78	with	grains	1183:1188	arg1	0.013					1277:1281	p = 0.013	1273:1281	p = 0.013	1273:1281	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	78	with	grains	1183:1188	arg1	p					1245:1245	p = 0.029	1245:1253	p = 0.029	1245:1253	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	78	with	grains	1183:1188	arg1	mass					1267:1270	muscle mass	1260:1270	muscle mass (p = 0.013)	1260:1282	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	78	with	grains	1183:1188	arg1	mass					1239:1242	bone mass	1234:1242	bone mass (p = 0.029)	1234:1254	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	3	79	theme	composition	569:579	arg1	pattern					532:538	pattern	532:538	pattern of food consumption and body composition in university students	532:602	This research aimed to determine the relationship between pattern of food consumption and body composition in university students.
33319575	13	80	theme	body	2103:2106	arg1	composition					2108:2118	body composition	2103:2118	body composition	2103:2118	Studies to establish associations between body composition and food consumption should be carried out taking into account specific intakes in order to establish a more accurate association.
33319575	1	81	theme	complex	203:209	arg1	lifestyles					211:220	complex lifestyles	203:220	complex lifestyles	203:220	Introduction: young university students face a social dynamics that has generated complex lifestyles and, especially, an imbalance in the intake of macro and micronutrients, characterized by having a high caloric density intake, which results in significant excess of weight.
33319575	4	82	theme	observational	616:628	arg1	study					660:664	an observational, descriptive, cross-sectional study	613:664	study	660:664	Method: an observational, descriptive, cross-sectional study with a sample of 189 students.
33319575	1	83	from	lifestyles	211:220	arg1	intake					259:264	the intake	255:264	the intake of macro and micronutrients	255:292	Introduction: young university students face a social dynamics that has generated complex lifestyles and, especially, an imbalance in the intake of macro and micronutrients, characterized by having a high caloric density intake, which results in significant excess of weight.
33319575	4	84	theme	189	683:685	arg1	students					687:694	189 students	683:694	189 students	683:694	Method: an observational, descriptive, cross-sectional study with a sample of 189 students.
33319575	3	85	from	pattern	532:538	arg1	university					584:593	university	584:593	university	584:593	This research aimed to determine the relationship between pattern of food consumption and body composition in university students.
33319575	8	86	dep	person	1462:1467	arg1	=					1473:1473	=	1473:1473	=	1473:1473	The multiple regression model showed that sugar consumption increases the risk of having visceral fat (OR = 2.71), and increases the probability of being classified as an obese person (OR = 2.55).
33319575	10	87	theme	water	1683:1687	arg1	percentage					1669:1678	a high percentage	1662:1678	a high percentage of water (OR = 5.41)	1662:1699	The consumption of fruits and vegetables increases the risk of having a high percentage of water (OR = 5.41), and low fat percentage (OR = 3.60).
33319575	10	87	theme	water	1683:1687	arg1	OR					1726:1727	OR = 3.60	1726:1734	OR = 3.60	1726:1734	The consumption of fruits and vegetables increases the risk of having a high percentage of water (OR = 5.41), and low fat percentage (OR = 3.60).
33319575	10	87	theme	water	1683:1687	arg1	percentage					1714:1723	low fat percentage	1706:1723	low fat percentage (OR = 3.60)	1706:1735	The consumption of fruits and vegetables increases the risk of having a high percentage of water (OR = 5.41), and low fat percentage (OR = 3.60).
33319575	1	88	theme	young	135:139	arg1	students					152:159	young university students	135:159	young university students	135:159	Introduction: young university students face a social dynamics that has generated complex lifestyles and, especially, an imbalance in the intake of macro and micronutrients, characterized by having a high caloric density intake, which results in significant excess of weight.
33319575	7	89	with	consumption	1207:1217	arg1	0.013					1277:1281	p = 0.013	1273:1281	p = 0.013	1273:1281	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	89	with	consumption	1207:1217	arg1	p					1245:1245	p = 0.029	1245:1253	p = 0.029	1245:1253	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	89	with	consumption	1207:1217	arg1	mass					1267:1270	muscle mass	1260:1270	muscle mass (p = 0.013)	1260:1282	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	89	with	consumption	1207:1217	arg1	mass					1239:1242	bone mass	1234:1242	bone mass (p = 0.029)	1234:1254	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	5	90	theme	body	746:749	arg1	composition					751:761	body composition	746:761	body composition variables obtained by bioimpedance	746:796	Sociodemographic variables obtained by a survey, body composition variables obtained by bioimpedance, and food groups and their categories obtained via a 24-hour reminder method were included.
33319575	6	91	theme	food	1000:1003	arg1	groups					1005:1010	food groups	1000:1010	food groups	1000:1010	A multinomial logistic regression model was performed to explain body composition according to consumption of food groups.
33319575	11	92	theme	visceral	1855:1862	arg1	fat					1864:1866	visceral fat	1855:1866	visceral fat	1855:1866	Conclusions: the consumption of sugars seems to have a close relationship with variables of body composition such as visceral fat, bone mass, and muscle mass.
33319575	12	93	theme	body	2049:2052	arg1	water					2054:2058	body water	2049:2058	body water	2049:2058	The consumption of fruits and vegetables was related to a low percentage of fat; however, their consumption is proportionally related to high levels of body water.
33319575	7	94	theme	=	1193:1193	arg1	sausages					1170:1177	sausages	1170:1177	sausages	1170:1177	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	94	theme	=	1193:1193	arg1	0.024					1195:1199	p = 0.024	1191:1199	p = 0.024	1191:1199	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	1	95	theme	caloric	326:332	arg1	intake					342:347	a high caloric density intake	319:347	a high caloric density intake	319:347	Introduction: young university students face a social dynamics that has generated complex lifestyles and, especially, an imbalance in the intake of macro and micronutrients, characterized by having a high caloric density intake, which results in significant excess of weight.
33319575	0	96	dep	study	113:117	arg1	[Relationship					0:12	[Relationship	0:12	[Relationship between pattern of food consumption and body composition in university students	0:92	[Relationship between pattern of food consumption and body composition in university students: a cross-sectional study].
33319575	9	97	dep	=	1583:1583	arg1	0.14					1585:1588	0.14	1585:1588	0.14	1585:1588	Consumption of eggs, meats, sausages, and grains favors an acceptable percentage of visceral fat (OR = 0.14).
33319575	7	98	theme	p	1273:1273	arg1	0.013					1277:1281	p = 0.013	1273:1281	p = 0.013	1273:1281	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	7	98	theme	p	1273:1273	arg1	mass					1267:1270	muscle mass	1260:1270	muscle mass (p = 0.013)	1260:1282	Results: statistically significant associations were obtained between food consumption and body composition for visceral fat and consumption of eggs, meats, sausages and grains (p = 0.024), and consumption of sugars with bone mass (p = 0.029) and muscle mass (p = 0.013).
33319575	12	99	theme	water	2054:2058	arg1	levels					2039:2044	high levels	2034:2044	high levels of body water	2034:2058	The consumption of fruits and vegetables was related to a low percentage of fat; however, their consumption is proportionally related to high levels of body water.
33319575	8	100	contain	having	1367:1372	arg1	risk					1359:1362	the risk	1355:1362	the risk of having visceral fat (OR = 2.71)	1355:1397	The multiple regression model showed that sugar consumption increases the risk of having visceral fat (OR = 2.71), and increases the probability of being classified as an obese person (OR = 2.55).
33319575	8	100	contain	having	1367:1372	arg2	2.71					1393:1396	= 2.71	1391:1396	= 2.71	1391:1396	The multiple regression model showed that sugar consumption increases the risk of having visceral fat (OR = 2.71), and increases the probability of being classified as an obese person (OR = 2.55).
33319575	8	100	contain	having	1367:1372	arg2	fat					1383:1385	visceral fat	1374:1385	visceral fat (OR = 2.71)	1374:1397	The multiple regression model showed that sugar consumption increases the risk of having visceral fat (OR = 2.71), and increases the probability of being classified as an obese person (OR = 2.55).
33319575	8	101	theme	obese	1456:1460	arg1	person					1462:1467	an obese person	1453:1467	an obese person (OR = 2.55)	1453:1479	The multiple regression model showed that sugar consumption increases the risk of having visceral fat (OR = 2.71), and increases the probability of being classified as an obese person (OR = 2.55).
33319575	8	102	theme	multiple	1289:1296	arg1	model					1309:1313	The multiple regression model	1285:1313	The multiple regression model	1285:1313	The multiple regression model showed that sugar consumption increases the risk of having visceral fat (OR = 2.71), and increases the probability of being classified as an obese person (OR = 2.55).
33319575	5	103	theme	food	803:806	arg1	groups					808:813	food groups	803:813	food groups	803:813	Sociodemographic variables obtained by a survey, body composition variables obtained by bioimpedance, and food groups and their categories obtained via a 24-hour reminder method were included.
33319575	4	104	with	study	660:664	arg1	sample					673:678	a sample	671:678	a sample of 189 students	671:694	Method: an observational, descriptive, cross-sectional study with a sample of 189 students.
33319575	12	105	theme	fat	1973:1975	arg1	percentage					1959:1968	a low percentage	1953:1968	a low percentage of fat	1953:1975	The consumption of fruits and vegetables was related to a low percentage of fat; however, their consumption is proportionally related to high levels of body water.
33319575	0	106	theme	consumption	38:48	arg1	pattern					22:28	pattern	22:28	pattern of food consumption and body composition in university students	22:92	[Relationship between pattern of food consumption and body composition in university students: a cross-sectional study].
33319575	1	107	theme	weight	389:394	arg1	excess					379:384	significant excess	367:384	significant excess of weight	367:394	Introduction: young university students face a social dynamics that has generated complex lifestyles and, especially, an imbalance in the intake of macro and micronutrients, characterized by having a high caloric density intake, which results in significant excess of weight.
33319575	0	108	theme	cross-sectional	97:111	arg1	study					113:117	a cross-sectional study	95:117	a cross-sectional study	95:117	[Relationship between pattern of food consumption and body composition in university students: a cross-sectional study].
33319575	0	109	theme	body	54:57	arg1	composition					59:69	body composition	54:69	body composition	54:69	[Relationship between pattern of food consumption and body composition in university students: a cross-sectional study].
33319575	1	110	from	imbalance	242:250	arg1	intake					259:264	the intake	255:264	the intake of macro and micronutrients	255:292	Introduction: young university students face a social dynamics that has generated complex lifestyles and, especially, an imbalance in the intake of macro and micronutrients, characterized by having a high caloric density intake, which results in significant excess of weight.
33319575	10	111	theme	low	1706:1708	arg1	OR					1726:1727	OR = 3.60	1726:1734	OR = 3.60	1726:1734	The consumption of fruits and vegetables increases the risk of having a high percentage of water (OR = 5.41), and low fat percentage (OR = 3.60).
33319575	10	111	theme	low	1706:1708	arg1	percentage					1714:1723	low fat percentage	1706:1723	low fat percentage (OR = 3.60)	1706:1735	The consumption of fruits and vegetables increases the risk of having a high percentage of water (OR = 5.41), and low fat percentage (OR = 3.60).
33319575	1	112	theme	significant	367:377	arg1	excess					379:384	significant excess	367:384	significant excess of weight	367:394	Introduction: young university students face a social dynamics that has generated complex lifestyles and, especially, an imbalance in the intake of macro and micronutrients, characterized by having a high caloric density intake, which results in significant excess of weight.
33319575	12	113	theme	fruits	1916:1921	arg1	consumption					1901:1911	The consumption	1897:1911	The consumption of fruits and vegetables	1897:1936	The consumption of fruits and vegetables was related to a low percentage of fat; however, their consumption is proportionally related to high levels of body water.
33319575	12	113	theme	fruits	1916:1921	arg1	related					1942:1948	related	1942:1948	related	1942:1948	The consumption of fruits and vegetables was related to a low percentage of fat; however, their consumption is proportionally related to high levels of body water.
33319575	13	114	theme	food	2124:2127	arg1	consumption					2129:2139	food consumption	2124:2139	food consumption	2124:2139	Studies to establish associations between body composition and food consumption should be carried out taking into account specific intakes in order to establish a more accurate association.
33319575	12	115	theme	vegetables	1927:1936	arg1	consumption					1901:1911	The consumption	1897:1911	The consumption of fruits and vegetables	1897:1936	The consumption of fruits and vegetables was related to a low percentage of fat; however, their consumption is proportionally related to high levels of body water.
33319575	12	115	theme	vegetables	1927:1936	arg1	related					1942:1948	related	1942:1948	related	1942:1948	The consumption of fruits and vegetables was related to a low percentage of fat; however, their consumption is proportionally related to high levels of body water.
33497213	3	0	with	microbiota	578:587	arg1	host					629:632	the host	625:632	the host	625:632	Microbiota composition and short chain fatty acids (SCFAs) in the colon and bile acids in the plasma of the rats were analyzed to assess the effects of P. sajor-caju supplementation on the microbiota in the colon and its interplay with the host in the event of hepatic steatosis.
33497213	3	1	from	composition	400:410	arg1	colon					455:459	the colon	451:459	the colon	451:459	Microbiota composition and short chain fatty acids (SCFAs) in the colon and bile acids in the plasma of the rats were analyzed to assess the effects of P. sajor-caju supplementation on the microbiota in the colon and its interplay with the host in the event of hepatic steatosis.
33497213	3	1	from	composition	400:410	arg1	plasma					483:488	the plasma	479:488	the plasma of the rats	479:500	Microbiota composition and short chain fatty acids (SCFAs) in the colon and bile acids in the plasma of the rats were analyzed to assess the effects of P. sajor-caju supplementation on the microbiota in the colon and its interplay with the host in the event of hepatic steatosis.
33497213	4	2	theme	P.	809:810	arg1	diet					836:839	the 5% P. sajor-caju-supplemented diet	802:839	the 5% P. sajor-caju-supplemented diet	802:839	Microbiota profiles were distinctly modulated by P. sajor-caju supplementation between the obese control rats and the obese rats fed the 5% P. sajor-caju-supplemented diet.
33497213	2	3	theme	oyster	349:354	arg1	mushroom					356:363	a biotechnologically produced oyster mushroom	319:363	a biotechnologically produced oyster mushroom (Pleurotus sajor-caju)	319:386	This study aimed to characterize the modulation of colonic microbiota in Zucker rats after supplementing their diet with a biotechnologically produced oyster mushroom (Pleurotus sajor-caju).
33497213	2	3	theme	oyster	349:354	arg1	sajor-caju					376:385	Pleurotus sajor-caju	366:385	Pleurotus sajor-caju	366:385	This study aimed to characterize the modulation of colonic microbiota in Zucker rats after supplementing their diet with a biotechnologically produced oyster mushroom (Pleurotus sajor-caju).
33497213	4	4	theme	sajor-caju	721:730	arg1	supplementation					732:746	P. sajor-caju supplementation	718:746	P. sajor-caju supplementation between the obese control rats and the obese rats fed the 5% P. sajor-caju-supplemented diet	718:839	Microbiota profiles were distinctly modulated by P. sajor-caju supplementation between the obese control rats and the obese rats fed the 5% P. sajor-caju-supplemented diet.
33497213	6	5	theme	steatosis	1289:1297	arg1	cases					1272:1276	cases	1272:1276	cases of hepatic steatosis and liver inflammation	1272:1320	This was also accompanied by distinct changes in the concentrations of bile acids in the plasma and concentrations of SCFAs in the colon, supporting the initial potentiality of P. sajor-caju as a prebiotic in cases of hepatic steatosis and liver inflammation.
33497213	4	6	theme	%	807:807	arg1	diet					836:839	the 5% P. sajor-caju-supplemented diet	802:839	the 5% P. sajor-caju-supplemented diet	802:839	Microbiota profiles were distinctly modulated by P. sajor-caju supplementation between the obese control rats and the obese rats fed the 5% P. sajor-caju-supplemented diet.
33497213	2	7	theme	produced	340:347	arg1	mushroom					356:363	a biotechnologically produced oyster mushroom	319:363	a biotechnologically produced oyster mushroom (Pleurotus sajor-caju)	319:386	This study aimed to characterize the modulation of colonic microbiota in Zucker rats after supplementing their diet with a biotechnologically produced oyster mushroom (Pleurotus sajor-caju).
33497213	2	7	theme	produced	340:347	arg1	sajor-caju					376:385	Pleurotus sajor-caju	366:385	Pleurotus sajor-caju	366:385	This study aimed to characterize the modulation of colonic microbiota in Zucker rats after supplementing their diet with a biotechnologically produced oyster mushroom (Pleurotus sajor-caju).
33497213	0	8	theme	Gut	89:91	arg1	Microbiota					93:102	Gut Microbiota	89:102	Gut Microbiota	89:102	Influence of a Biotechnologically Produced Oyster Mushroom (Pleurotus sajor-caju) on the Gut Microbiota and Microbial Metabolites in Obese Zucker Rats.
33497213	0	9	from	Influence	0:8	arg1	Microbiota					93:102	Gut Microbiota	89:102	Gut Microbiota	89:102	Influence of a Biotechnologically Produced Oyster Mushroom (Pleurotus sajor-caju) on the Gut Microbiota and Microbial Metabolites in Obese Zucker Rats.
33497213	0	9	from	Influence	0:8	arg1	Metabolites					118:128	Microbial Metabolites	108:128	Microbial Metabolites	108:128	Influence of a Biotechnologically Produced Oyster Mushroom (Pleurotus sajor-caju) on the Gut Microbiota and Microbial Metabolites in Obese Zucker Rats.
33497213	6	10	theme	P.	1240:1241	arg1	sajor-caju					1243:1252	P. sajor-caju	1240:1252	P. sajor-caju	1240:1252	This was also accompanied by distinct changes in the concentrations of bile acids in the plasma and concentrations of SCFAs in the colon, supporting the initial potentiality of P. sajor-caju as a prebiotic in cases of hepatic steatosis and liver inflammation.
33497213	6	11	theme	acids	1139:1143	arg1	concentrations					1116:1129	the concentrations	1112:1129	the concentrations of bile acids in the plasma and concentrations of SCFAs	1112:1185	This was also accompanied by distinct changes in the concentrations of bile acids in the plasma and concentrations of SCFAs in the colon, supporting the initial potentiality of P. sajor-caju as a prebiotic in cases of hepatic steatosis and liver inflammation.
33497213	2	12	theme	Zucker	271:276	arg1	rats					278:281	Zucker rats	271:281	Zucker rats	271:281	This study aimed to characterize the modulation of colonic microbiota in Zucker rats after supplementing their diet with a biotechnologically produced oyster mushroom (Pleurotus sajor-caju).
33497213	6	13	theme	bile	1134:1137	arg1	acids					1139:1143	bile acids	1134:1143	bile acids in the plasma and concentrations of SCFAs	1134:1185	This was also accompanied by distinct changes in the concentrations of bile acids in the plasma and concentrations of SCFAs in the colon, supporting the initial potentiality of P. sajor-caju as a prebiotic in cases of hepatic steatosis and liver inflammation.
33497213	4	14	theme	sajor-caju-supplemented	812:834	arg1	diet					836:839	the 5% P. sajor-caju-supplemented diet	802:839	the 5% P. sajor-caju-supplemented diet	802:839	Microbiota profiles were distinctly modulated by P. sajor-caju supplementation between the obese control rats and the obese rats fed the 5% P. sajor-caju-supplemented diet.
33497213	6	15	dep	plasma	1152:1157	arg1	the					1148:1150	the	1148:1150	the	1148:1150	This was also accompanied by distinct changes in the concentrations of bile acids in the plasma and concentrations of SCFAs in the colon, supporting the initial potentiality of P. sajor-caju as a prebiotic in cases of hepatic steatosis and liver inflammation.
33497213	0	16	dep	Microbiota	93:102	arg1	the					85:87	the	85:87	the	85:87	Influence of a Biotechnologically Produced Oyster Mushroom (Pleurotus sajor-caju) on the Gut Microbiota and Microbial Metabolites in Obese Zucker Rats.
33497213	3	17	with	interplay	610:618	arg1	host					629:632	the host	625:632	the host	625:632	Microbiota composition and short chain fatty acids (SCFAs) in the colon and bile acids in the plasma of the rats were analyzed to assess the effects of P. sajor-caju supplementation on the microbiota in the colon and its interplay with the host in the event of hepatic steatosis.
33497213	6	18	theme	inflammation	1309:1320	arg1	cases					1272:1276	cases	1272:1276	cases of hepatic steatosis and liver inflammation	1272:1320	This was also accompanied by distinct changes in the concentrations of bile acids in the plasma and concentrations of SCFAs in the colon, supporting the initial potentiality of P. sajor-caju as a prebiotic in cases of hepatic steatosis and liver inflammation.
33497213	6	19	theme	initial	1216:1222	arg1	potentiality					1224:1235	the initial potentiality	1212:1235	the initial potentiality of P. sajor-caju	1212:1252	This was also accompanied by distinct changes in the concentrations of bile acids in the plasma and concentrations of SCFAs in the colon, supporting the initial potentiality of P. sajor-caju as a prebiotic in cases of hepatic steatosis and liver inflammation.
33497213	6	19	theme	initial	1216:1222	arg1	prebiotic					1259:1267	a prebiotic	1257:1267	a prebiotic in cases of hepatic steatosis and liver inflammation	1257:1320	This was also accompanied by distinct changes in the concentrations of bile acids in the plasma and concentrations of SCFAs in the colon, supporting the initial potentiality of P. sajor-caju as a prebiotic in cases of hepatic steatosis and liver inflammation.
33497213	2	20	theme	microbiota	257:266	arg1	modulation					235:244	the modulation	231:244	the modulation of colonic microbiota in Zucker rats	231:281	This study aimed to characterize the modulation of colonic microbiota in Zucker rats after supplementing their diet with a biotechnologically produced oyster mushroom (Pleurotus sajor-caju).
33497213	3	21	theme	chain	422:426	arg1	acids					434:438	short chain fatty acids	416:438	short chain fatty acids (SCFAs) in the colon	416:459	Microbiota composition and short chain fatty acids (SCFAs) in the colon and bile acids in the plasma of the rats were analyzed to assess the effects of P. sajor-caju supplementation on the microbiota in the colon and its interplay with the host in the event of hepatic steatosis.
33497213	3	21	theme	chain	422:426	arg1	SCFAs					441:445	SCFAs	441:445	SCFAs	441:445	Microbiota composition and short chain fatty acids (SCFAs) in the colon and bile acids in the plasma of the rats were analyzed to assess the effects of P. sajor-caju supplementation on the microbiota in the colon and its interplay with the host in the event of hepatic steatosis.
33497213	6	22	theme	liver	1303:1307	arg1	inflammation					1309:1320	liver inflammation	1303:1320	liver inflammation	1303:1320	This was also accompanied by distinct changes in the concentrations of bile acids in the plasma and concentrations of SCFAs in the colon, supporting the initial potentiality of P. sajor-caju as a prebiotic in cases of hepatic steatosis and liver inflammation.
33497213	3	23	theme	hepatic	650:656	arg1	steatosis					658:666	hepatic steatosis	650:666	hepatic steatosis	650:666	Microbiota composition and short chain fatty acids (SCFAs) in the colon and bile acids in the plasma of the rats were analyzed to assess the effects of P. sajor-caju supplementation on the microbiota in the colon and its interplay with the host in the event of hepatic steatosis.
33497213	3	24	theme	fatty	428:432	arg1	acids					434:438	short chain fatty acids	416:438	short chain fatty acids (SCFAs) in the colon	416:459	Microbiota composition and short chain fatty acids (SCFAs) in the colon and bile acids in the plasma of the rats were analyzed to assess the effects of P. sajor-caju supplementation on the microbiota in the colon and its interplay with the host in the event of hepatic steatosis.
33497213	3	24	theme	fatty	428:432	arg1	SCFAs					441:445	SCFAs	441:445	SCFAs	441:445	Microbiota composition and short chain fatty acids (SCFAs) in the colon and bile acids in the plasma of the rats were analyzed to assess the effects of P. sajor-caju supplementation on the microbiota in the colon and its interplay with the host in the event of hepatic steatosis.
33497213	2	25	theme	colonic	249:255	arg1	microbiota					257:266	colonic microbiota	249:266	colonic microbiota	249:266	This study aimed to characterize the modulation of colonic microbiota in Zucker rats after supplementing their diet with a biotechnologically produced oyster mushroom (Pleurotus sajor-caju).
33497213	3	26	from	microbiota	578:587	arg1	colon					596:600	the colon	592:600	the colon	592:600	Microbiota composition and short chain fatty acids (SCFAs) in the colon and bile acids in the plasma of the rats were analyzed to assess the effects of P. sajor-caju supplementation on the microbiota in the colon and its interplay with the host in the event of hepatic steatosis.
33497213	4	27	theme	5	806:806	arg1	%					807:807	%	807:807	%	807:807	Microbiota profiles were distinctly modulated by P. sajor-caju supplementation between the obese control rats and the obese rats fed the 5% P. sajor-caju-supplemented diet.
33497213	5	28	theme	bacterial	895:903	arg1	genera					905:910	SCFAs-producing bacterial genera	879:910	SCFAs-producing bacterial genera	879:910	P. sajor-caju enhanced the growth of SCFAs-producing bacterial genera, including Faecalibaculum, Bifidobacterium, Roseburia, and Blautia, and decreased the relative abundance of the pathogenic genus Escherichia-Shigella.
33497213	1	29	theme	rich	168:171	arg1	source					173:178	a rich source	166:178	a rich source of dietary fiber	166:195	Mushrooms are a rich source of dietary fiber.
33497213	1	29	theme	rich	168:171	arg1	Mushrooms					152:160	Mushrooms	152:160	Mushrooms	152:160	Mushrooms are a rich source of dietary fiber.
33497213	6	30	theme	hepatic	1281:1287	arg1	steatosis					1289:1297	hepatic steatosis	1281:1297	hepatic steatosis	1281:1297	This was also accompanied by distinct changes in the concentrations of bile acids in the plasma and concentrations of SCFAs in the colon, supporting the initial potentiality of P. sajor-caju as a prebiotic in cases of hepatic steatosis and liver inflammation.
33497213	5	31	theme	genera	905:910	arg1	growth					869:874	the growth	865:874	the growth of SCFAs-producing bacterial genera, including Faecalibaculum, Bifidobacterium, Roseburia, and Blautia,	865:978	P. sajor-caju enhanced the growth of SCFAs-producing bacterial genera, including Faecalibaculum, Bifidobacterium, Roseburia, and Blautia, and decreased the relative abundance of the pathogenic genus Escherichia-Shigella.
33497213	6	32	from	concentrations	1116:1129	arg1	concentrations					1163:1176	concentrations	1163:1176	concentrations	1163:1176	This was also accompanied by distinct changes in the concentrations of bile acids in the plasma and concentrations of SCFAs in the colon, supporting the initial potentiality of P. sajor-caju as a prebiotic in cases of hepatic steatosis and liver inflammation.
33497213	6	32	from	concentrations	1116:1129	arg1	plasma					1152:1157	plasma	1152:1157	plasma	1152:1157	This was also accompanied by distinct changes in the concentrations of bile acids in the plasma and concentrations of SCFAs in the colon, supporting the initial potentiality of P. sajor-caju as a prebiotic in cases of hepatic steatosis and liver inflammation.
33497213	2	33	theme	Pleurotus	366:374	arg1	mushroom					356:363	a biotechnologically produced oyster mushroom	319:363	a biotechnologically produced oyster mushroom (Pleurotus sajor-caju)	319:386	This study aimed to characterize the modulation of colonic microbiota in Zucker rats after supplementing their diet with a biotechnologically produced oyster mushroom (Pleurotus sajor-caju).
33497213	2	33	theme	Pleurotus	366:374	arg1	sajor-caju					376:385	Pleurotus sajor-caju	366:385	Pleurotus sajor-caju	366:385	This study aimed to characterize the modulation of colonic microbiota in Zucker rats after supplementing their diet with a biotechnologically produced oyster mushroom (Pleurotus sajor-caju).
33497213	3	34	from	acids	470:474	arg1	colon					455:459	the colon	451:459	the colon	451:459	Microbiota composition and short chain fatty acids (SCFAs) in the colon and bile acids in the plasma of the rats were analyzed to assess the effects of P. sajor-caju supplementation on the microbiota in the colon and its interplay with the host in the event of hepatic steatosis.
33497213	3	34	from	acids	470:474	arg1	plasma					483:488	the plasma	479:488	the plasma of the rats	479:500	Microbiota composition and short chain fatty acids (SCFAs) in the colon and bile acids in the plasma of the rats were analyzed to assess the effects of P. sajor-caju supplementation on the microbiota in the colon and its interplay with the host in the event of hepatic steatosis.
33497213	0	35	theme	Microbial	108:116	arg1	Metabolites					118:128	Microbial Metabolites	108:128	Microbial Metabolites	108:128	Influence of a Biotechnologically Produced Oyster Mushroom (Pleurotus sajor-caju) on the Gut Microbiota and Microbial Metabolites in Obese Zucker Rats.
33497213	5	36	theme	pathogenic	1024:1033	arg1	Escherichia-Shigella					1041:1060	the pathogenic genus Escherichia-Shigella	1020:1060	the pathogenic genus Escherichia-Shigella	1020:1060	P. sajor-caju enhanced the growth of SCFAs-producing bacterial genera, including Faecalibaculum, Bifidobacterium, Roseburia, and Blautia, and decreased the relative abundance of the pathogenic genus Escherichia-Shigella.
33497213	1	37	theme	dietary	183:189	arg1	fiber					191:195	dietary fiber	183:195	dietary fiber	183:195	Mushrooms are a rich source of dietary fiber.
33497213	0	38	theme	Oyster	43:48	arg1	Mushroom					50:57	a Biotechnologically Produced Oyster Mushroom	13:57	a Biotechnologically Produced Oyster Mushroom (Pleurotus sajor-caju)	13:80	Influence of a Biotechnologically Produced Oyster Mushroom (Pleurotus sajor-caju) on the Gut Microbiota and Microbial Metabolites in Obese Zucker Rats.
33497213	0	38	theme	Oyster	43:48	arg1	sajor-caju					70:79	Pleurotus sajor-caju	60:79	Pleurotus sajor-caju	60:79	Influence of a Biotechnologically Produced Oyster Mushroom (Pleurotus sajor-caju) on the Gut Microbiota and Microbial Metabolites in Obese Zucker Rats.
33497213	6	39	from	acids	1139:1143	arg1	concentrations					1163:1176	concentrations	1163:1176	concentrations	1163:1176	This was also accompanied by distinct changes in the concentrations of bile acids in the plasma and concentrations of SCFAs in the colon, supporting the initial potentiality of P. sajor-caju as a prebiotic in cases of hepatic steatosis and liver inflammation.
33497213	6	39	from	acids	1139:1143	arg1	plasma					1152:1157	plasma	1152:1157	plasma	1152:1157	This was also accompanied by distinct changes in the concentrations of bile acids in the plasma and concentrations of SCFAs in the colon, supporting the initial potentiality of P. sajor-caju as a prebiotic in cases of hepatic steatosis and liver inflammation.
33497213	0	40	theme	Obese	133:137	arg1	Rats					146:149	Obese Zucker Rats	133:149	Obese Zucker Rats	133:149	Influence of a Biotechnologically Produced Oyster Mushroom (Pleurotus sajor-caju) on the Gut Microbiota and Microbial Metabolites in Obese Zucker Rats.
33497213	5	41	theme	genus	1035:1039	arg1	Escherichia-Shigella					1041:1060	the pathogenic genus Escherichia-Shigella	1020:1060	the pathogenic genus Escherichia-Shigella	1020:1060	P. sajor-caju enhanced the growth of SCFAs-producing bacterial genera, including Faecalibaculum, Bifidobacterium, Roseburia, and Blautia, and decreased the relative abundance of the pathogenic genus Escherichia-Shigella.
33497213	3	42	theme	sajor-caju	544:553	arg1	supplementation					555:569	P. sajor-caju supplementation	541:569	P. sajor-caju supplementation	541:569	Microbiota composition and short chain fatty acids (SCFAs) in the colon and bile acids in the plasma of the rats were analyzed to assess the effects of P. sajor-caju supplementation on the microbiota in the colon and its interplay with the host in the event of hepatic steatosis.
33497213	2	43	from	modulation	235:244	arg1	rats					278:281	Zucker rats	271:281	Zucker rats	271:281	This study aimed to characterize the modulation of colonic microbiota in Zucker rats after supplementing their diet with a biotechnologically produced oyster mushroom (Pleurotus sajor-caju).
33497213	1	44	theme	fiber	191:195	arg1	source					173:178	a rich source	166:178	a rich source of dietary fiber	166:195	Mushrooms are a rich source of dietary fiber.
33497213	1	44	theme	fiber	191:195	arg1	Mushrooms					152:160	Mushrooms	152:160	Mushrooms	152:160	Mushrooms are a rich source of dietary fiber.
33497213	0	45	theme	Produced	34:41	arg1	Mushroom					50:57	a Biotechnologically Produced Oyster Mushroom	13:57	a Biotechnologically Produced Oyster Mushroom (Pleurotus sajor-caju)	13:80	Influence of a Biotechnologically Produced Oyster Mushroom (Pleurotus sajor-caju) on the Gut Microbiota and Microbial Metabolites in Obese Zucker Rats.
33497213	0	45	theme	Produced	34:41	arg1	sajor-caju					70:79	Pleurotus sajor-caju	60:79	Pleurotus sajor-caju	60:79	Influence of a Biotechnologically Produced Oyster Mushroom (Pleurotus sajor-caju) on the Gut Microbiota and Microbial Metabolites in Obese Zucker Rats.
33497213	6	46	from	prebiotic	1259:1267	arg1	cases					1272:1276	cases	1272:1276	cases of hepatic steatosis and liver inflammation	1272:1320	This was also accompanied by distinct changes in the concentrations of bile acids in the plasma and concentrations of SCFAs in the colon, supporting the initial potentiality of P. sajor-caju as a prebiotic in cases of hepatic steatosis and liver inflammation.
33497213	3	47	theme	short	416:420	arg1	acids					434:438	short chain fatty acids	416:438	short chain fatty acids (SCFAs) in the colon	416:459	Microbiota composition and short chain fatty acids (SCFAs) in the colon and bile acids in the plasma of the rats were analyzed to assess the effects of P. sajor-caju supplementation on the microbiota in the colon and its interplay with the host in the event of hepatic steatosis.
33497213	3	47	theme	short	416:420	arg1	SCFAs					441:445	SCFAs	441:445	SCFAs	441:445	Microbiota composition and short chain fatty acids (SCFAs) in the colon and bile acids in the plasma of the rats were analyzed to assess the effects of P. sajor-caju supplementation on the microbiota in the colon and its interplay with the host in the event of hepatic steatosis.
33497213	5	48	theme	Escherichia-Shigella	1041:1060	arg1	abundance					1007:1015	the relative abundance	994:1015	the relative abundance of the pathogenic genus Escherichia-Shigella	994:1060	P. sajor-caju enhanced the growth of SCFAs-producing bacterial genera, including Faecalibaculum, Bifidobacterium, Roseburia, and Blautia, and decreased the relative abundance of the pathogenic genus Escherichia-Shigella.
33497213	3	49	theme	supplementation	555:569	arg1	effects					530:536	the effects	526:536	the effects of P. sajor-caju supplementation on the microbiota in the colon and its interplay with the host	526:632	Microbiota composition and short chain fatty acids (SCFAs) in the colon and bile acids in the plasma of the rats were analyzed to assess the effects of P. sajor-caju supplementation on the microbiota in the colon and its interplay with the host in the event of hepatic steatosis.
33497213	3	50	theme	Microbiota	389:398	arg1	composition					400:410	Microbiota composition	389:410	Microbiota composition	389:410	Microbiota composition and short chain fatty acids (SCFAs) in the colon and bile acids in the plasma of the rats were analyzed to assess the effects of P. sajor-caju supplementation on the microbiota in the colon and its interplay with the host in the event of hepatic steatosis.
33497213	3	51	dep	steatosis	658:666	arg1	event					641:645	event	641:645	event	641:645	Microbiota composition and short chain fatty acids (SCFAs) in the colon and bile acids in the plasma of the rats were analyzed to assess the effects of P. sajor-caju supplementation on the microbiota in the colon and its interplay with the host in the event of hepatic steatosis.
33497213	3	51	dep	steatosis	658:666	arg1	the					637:639	the	637:639	the	637:639	Microbiota composition and short chain fatty acids (SCFAs) in the colon and bile acids in the plasma of the rats were analyzed to assess the effects of P. sajor-caju supplementation on the microbiota in the colon and its interplay with the host in the event of hepatic steatosis.
33497213	4	52	theme	obese	787:791	arg1	rats					793:796	the obese rats	783:796	the obese rats fed the 5% P. sajor-caju-supplemented diet	783:839	Microbiota profiles were distinctly modulated by P. sajor-caju supplementation between the obese control rats and the obese rats fed the 5% P. sajor-caju-supplemented diet.
33497213	3	53	theme	bile	465:468	arg1	acids					470:474	bile acids	465:474	bile acids	465:474	Microbiota composition and short chain fatty acids (SCFAs) in the colon and bile acids in the plasma of the rats were analyzed to assess the effects of P. sajor-caju supplementation on the microbiota in the colon and its interplay with the host in the event of hepatic steatosis.
33497213	0	54	theme	Mushroom	50:57	arg1	Influence					0:8	Influence	0:8	Influence of a Biotechnologically Produced Oyster Mushroom (Pleurotus sajor-caju) on the Gut Microbiota and Microbial Metabolites in Obese Zucker Rats.	0:150	Influence of a Biotechnologically Produced Oyster Mushroom (Pleurotus sajor-caju) on the Gut Microbiota and Microbial Metabolites in Obese Zucker Rats.
33497213	0	55	theme	Zucker	139:144	arg1	Rats					146:149	Obese Zucker Rats	133:149	Obese Zucker Rats	133:149	Influence of a Biotechnologically Produced Oyster Mushroom (Pleurotus sajor-caju) on the Gut Microbiota and Microbial Metabolites in Obese Zucker Rats.
33497213	5	56	theme	relative	998:1005	arg1	abundance					1007:1015	the relative abundance	994:1015	the relative abundance of the pathogenic genus Escherichia-Shigella	994:1060	P. sajor-caju enhanced the growth of SCFAs-producing bacterial genera, including Faecalibaculum, Bifidobacterium, Roseburia, and Blautia, and decreased the relative abundance of the pathogenic genus Escherichia-Shigella.
33497213	3	57	theme	P.	541:542	arg1	supplementation					555:569	P. sajor-caju supplementation	541:569	P. sajor-caju supplementation	541:569	Microbiota composition and short chain fatty acids (SCFAs) in the colon and bile acids in the plasma of the rats were analyzed to assess the effects of P. sajor-caju supplementation on the microbiota in the colon and its interplay with the host in the event of hepatic steatosis.
33497213	6	58	theme	sajor-caju	1243:1252	arg1	potentiality					1224:1235	the initial potentiality	1212:1235	the initial potentiality of P. sajor-caju	1212:1252	This was also accompanied by distinct changes in the concentrations of bile acids in the plasma and concentrations of SCFAs in the colon, supporting the initial potentiality of P. sajor-caju as a prebiotic in cases of hepatic steatosis and liver inflammation.
33497213	6	58	theme	sajor-caju	1243:1252	arg1	prebiotic					1259:1267	a prebiotic	1257:1267	a prebiotic in cases of hepatic steatosis and liver inflammation	1257:1320	This was also accompanied by distinct changes in the concentrations of bile acids in the plasma and concentrations of SCFAs in the colon, supporting the initial potentiality of P. sajor-caju as a prebiotic in cases of hepatic steatosis and liver inflammation.
33497213	0	59	from	Microbiota	93:102	arg1	Rats					146:149	Obese Zucker Rats	133:149	Obese Zucker Rats	133:149	Influence of a Biotechnologically Produced Oyster Mushroom (Pleurotus sajor-caju) on the Gut Microbiota and Microbial Metabolites in Obese Zucker Rats.
33497213	6	60	theme	SCFAs	1181:1185	arg1	concentrations					1163:1176	concentrations	1163:1176	concentrations	1163:1176	This was also accompanied by distinct changes in the concentrations of bile acids in the plasma and concentrations of SCFAs in the colon, supporting the initial potentiality of P. sajor-caju as a prebiotic in cases of hepatic steatosis and liver inflammation.
33497213	6	60	theme	SCFAs	1181:1185	arg1	plasma					1152:1157	plasma	1152:1157	plasma	1152:1157	This was also accompanied by distinct changes in the concentrations of bile acids in the plasma and concentrations of SCFAs in the colon, supporting the initial potentiality of P. sajor-caju as a prebiotic in cases of hepatic steatosis and liver inflammation.
33497213	6	61	from	changes	1101:1107	arg1	colon					1194:1198	the colon	1190:1198	the colon	1190:1198	This was also accompanied by distinct changes in the concentrations of bile acids in the plasma and concentrations of SCFAs in the colon, supporting the initial potentiality of P. sajor-caju as a prebiotic in cases of hepatic steatosis and liver inflammation.
33497213	6	61	from	changes	1101:1107	arg1	concentrations					1116:1129	the concentrations	1112:1129	the concentrations of bile acids in the plasma and concentrations of SCFAs	1112:1185	This was also accompanied by distinct changes in the concentrations of bile acids in the plasma and concentrations of SCFAs in the colon, supporting the initial potentiality of P. sajor-caju as a prebiotic in cases of hepatic steatosis and liver inflammation.
33497213	4	62	theme	Microbiota	669:678	arg1	profiles					680:687	Microbiota profiles	669:687	Microbiota profiles	669:687	Microbiota profiles were distinctly modulated by P. sajor-caju supplementation between the obese control rats and the obese rats fed the 5% P. sajor-caju-supplemented diet.
33497213	3	63	theme	rats	497:500	arg1	plasma					483:488	the plasma	479:488	the plasma of the rats	479:500	Microbiota composition and short chain fatty acids (SCFAs) in the colon and bile acids in the plasma of the rats were analyzed to assess the effects of P. sajor-caju supplementation on the microbiota in the colon and its interplay with the host in the event of hepatic steatosis.
33497213	4	64	theme	P.	718:719	arg1	supplementation					732:746	P. sajor-caju supplementation	718:746	P. sajor-caju supplementation between the obese control rats and the obese rats fed the 5% P. sajor-caju-supplemented diet	718:839	Microbiota profiles were distinctly modulated by P. sajor-caju supplementation between the obese control rats and the obese rats fed the 5% P. sajor-caju-supplemented diet.
33497213	5	65	theme	SCFAs-producing	879:893	arg1	genera					905:910	SCFAs-producing bacterial genera	879:910	SCFAs-producing bacterial genera	879:910	P. sajor-caju enhanced the growth of SCFAs-producing bacterial genera, including Faecalibaculum, Bifidobacterium, Roseburia, and Blautia, and decreased the relative abundance of the pathogenic genus Escherichia-Shigella.
33497213	6	66	theme	distinct	1092:1099	arg1	changes					1101:1107	distinct changes	1092:1107	distinct changes in the concentrations of bile acids in the plasma and concentrations of SCFAs in the colon	1092:1198	This was also accompanied by distinct changes in the concentrations of bile acids in the plasma and concentrations of SCFAs in the colon, supporting the initial potentiality of P. sajor-caju as a prebiotic in cases of hepatic steatosis and liver inflammation.
33497213	3	67	from	interplay	610:618	arg1	colon					596:600	the colon	592:600	the colon	592:600	Microbiota composition and short chain fatty acids (SCFAs) in the colon and bile acids in the plasma of the rats were analyzed to assess the effects of P. sajor-caju supplementation on the microbiota in the colon and its interplay with the host in the event of hepatic steatosis.
33497213	6	68	from	plasma	1152:1157	arg1	concentrations					1116:1129	the concentrations	1112:1129	the concentrations of bile acids in the plasma and concentrations of SCFAs	1112:1185	This was also accompanied by distinct changes in the concentrations of bile acids in the plasma and concentrations of SCFAs in the colon, supporting the initial potentiality of P. sajor-caju as a prebiotic in cases of hepatic steatosis and liver inflammation.
33497213	4	69	theme	control	766:772	arg1	rats					774:777	the obese control rats	756:777	the obese control rats	756:777	Microbiota profiles were distinctly modulated by P. sajor-caju supplementation between the obese control rats and the obese rats fed the 5% P. sajor-caju-supplemented diet.
33497213	3	70	from	effects	530:536	arg1	microbiota					578:587	the microbiota	574:587	the microbiota in the colon	574:600	Microbiota composition and short chain fatty acids (SCFAs) in the colon and bile acids in the plasma of the rats were analyzed to assess the effects of P. sajor-caju supplementation on the microbiota in the colon and its interplay with the host in the event of hepatic steatosis.
33497213	3	70	from	effects	530:536	arg1	interplay					610:618	its interplay	606:618	its interplay with the host	606:632	Microbiota composition and short chain fatty acids (SCFAs) in the colon and bile acids in the plasma of the rats were analyzed to assess the effects of P. sajor-caju supplementation on the microbiota in the colon and its interplay with the host in the event of hepatic steatosis.
33497213	0	71	from	Metabolites	118:128	arg1	Rats					146:149	Obese Zucker Rats	133:149	Obese Zucker Rats	133:149	Influence of a Biotechnologically Produced Oyster Mushroom (Pleurotus sajor-caju) on the Gut Microbiota and Microbial Metabolites in Obese Zucker Rats.
33497213	0	72	theme	Pleurotus	60:68	arg1	Mushroom					50:57	a Biotechnologically Produced Oyster Mushroom	13:57	a Biotechnologically Produced Oyster Mushroom (Pleurotus sajor-caju)	13:80	Influence of a Biotechnologically Produced Oyster Mushroom (Pleurotus sajor-caju) on the Gut Microbiota and Microbial Metabolites in Obese Zucker Rats.
33497213	0	72	theme	Pleurotus	60:68	arg1	sajor-caju					70:79	Pleurotus sajor-caju	60:79	Pleurotus sajor-caju	60:79	Influence of a Biotechnologically Produced Oyster Mushroom (Pleurotus sajor-caju) on the Gut Microbiota and Microbial Metabolites in Obese Zucker Rats.
33497213	4	73	theme	obese	760:764	arg1	rats					774:777	the obese control rats	756:777	the obese control rats	756:777	Microbiota profiles were distinctly modulated by P. sajor-caju supplementation between the obese control rats and the obese rats fed the 5% P. sajor-caju-supplemented diet.
33497213	3	74	from	acids	434:438	arg1	colon					455:459	the colon	451:459	the colon	451:459	Microbiota composition and short chain fatty acids (SCFAs) in the colon and bile acids in the plasma of the rats were analyzed to assess the effects of P. sajor-caju supplementation on the microbiota in the colon and its interplay with the host in the event of hepatic steatosis.
33497213	3	74	from	acids	434:438	arg1	plasma					483:488	the plasma	479:488	the plasma of the rats	479:500	Microbiota composition and short chain fatty acids (SCFAs) in the colon and bile acids in the plasma of the rats were analyzed to assess the effects of P. sajor-caju supplementation on the microbiota in the colon and its interplay with the host in the event of hepatic steatosis.
33497213	5	75	theme	P.	842:843	arg1	sajor-caju					845:854	P. sajor-caju	842:854	P. sajor-caju	842:854	P. sajor-caju enhanced the growth of SCFAs-producing bacterial genera, including Faecalibaculum, Bifidobacterium, Roseburia, and Blautia, and decreased the relative abundance of the pathogenic genus Escherichia-Shigella.
33497213	6	76	from	concentrations	1163:1176	arg1	concentrations					1116:1129	the concentrations	1112:1129	the concentrations of bile acids in the plasma and concentrations of SCFAs	1112:1185	This was also accompanied by distinct changes in the concentrations of bile acids in the plasma and concentrations of SCFAs in the colon, supporting the initial potentiality of P. sajor-caju as a prebiotic in cases of hepatic steatosis and liver inflammation.
32724983	6	0	theme	low	1072:1074	arg1	concentrations					1080:1093	low PEU concentrations	1072:1093	low PEU concentrations (1-5% w/v)	1072:1104	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties was observed: the first were predominant at low PEU concentrations (1-5% w/v), while the latter prevailed at high PEU concentrations (7-9% w/v).
32724983	6	0	theme	low	1072:1074	arg1	w/v					1101:1103	1-5% w/v	1096:1103	1-5% w/v	1096:1103	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties was observed: the first were predominant at low PEU concentrations (1-5% w/v), while the latter prevailed at high PEU concentrations (7-9% w/v).
32724983	1	1	theme	effective	240:248	arg1	strategy					250:257	an effective strategy	237:257	an effective strategy to develop drug delivery systems	237:290	The design of supramolecular (SM) hydrogels based on host-guest complexes represents an effective strategy to develop drug delivery systems.
32724983	6	2	theme	high	1137:1140	arg1	w/v					1167:1169	7-9% w/v	1162:1169	7-9% w/v	1162:1169	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties was observed: the first were predominant at low PEU concentrations (1-5% w/v), while the latter prevailed at high PEU concentrations (7-9% w/v).
32724983	6	2	theme	high	1137:1140	arg1	concentrations					1146:1159	high PEU concentrations	1137:1159	high PEU concentrations (7-9% w/v)	1137:1170	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties was observed: the first were predominant at low PEU concentrations (1-5% w/v), while the latter prevailed at high PEU concentrations (7-9% w/v).
32724983	9	3	theme	3-4	1814:1816	arg1	days					1818:1821	3-4 days	1814:1821	3-4 days with no initial burst release	1814:1851	Gels progressively released a model molecule (fluorescein isothiocyanate-dextran) within 3-4 days with no initial burst release.
32724983	10	4	with	hydrogels	1932:1940	arg1	α-cyclodextrin					1947:1960	α-cyclodextrin	1947:1960	α-cyclodextrin	1947:1960	We thus demonstrated the suitability of custom-made PEUs as constituent of SM hydrogels with α-cyclodextrin and the high potential of the resulting systems for drug delivery applications.
32724983	10	4	with	hydrogels	1932:1940	arg1	potential					1975:1983	the high potential	1966:1983	the high potential of the resulting systems for drug delivery applications	1966:2039	We thus demonstrated the suitability of custom-made PEUs as constituent of SM hydrogels with α-cyclodextrin and the high potential of the resulting systems for drug delivery applications.
32724983	7	5	with	gels	1209:1212	arg1	networks					1267:1274	not-fully developed networks	1247:1274	not-fully developed networks	1247:1274	Increasing PEU concentration led to gels with increased dissolution rate, not-fully developed networks and slight cytotoxicity, meanwhile residence time in aqueous media improved (>7 d).
32724983	7	5	with	gels	1209:1212	arg1	cytotoxicity					1287:1298	slight cytotoxicity	1280:1298	slight cytotoxicity	1280:1298	Increasing PEU concentration led to gels with increased dissolution rate, not-fully developed networks and slight cytotoxicity, meanwhile residence time in aqueous media improved (>7 d).
32724983	7	5	with	gels	1209:1212	arg1	d					1356:1356	>7 d	1353:1356	>7 d	1353:1356	Increasing PEU concentration led to gels with increased dissolution rate, not-fully developed networks and slight cytotoxicity, meanwhile residence time in aqueous media improved (>7 d).
32724983	7	5	with	gels	1209:1212	arg1	rate					1241:1244	increased dissolution rate	1219:1244	increased dissolution rate	1219:1244	Increasing PEU concentration led to gels with increased dissolution rate, not-fully developed networks and slight cytotoxicity, meanwhile residence time in aqueous media improved (>7 d).
32724983	7	5	with	gels	1209:1212	arg1	time					1321:1324	meanwhile residence time	1301:1324	meanwhile residence time in aqueous media improved	1301:1350	Increasing PEU concentration led to gels with increased dissolution rate, not-fully developed networks and slight cytotoxicity, meanwhile residence time in aqueous media improved (>7 d).
32724983	9	6	theme	initial	1831:1837	arg1	release					1845:1851	no initial burst release	1828:1851	no initial burst release	1828:1851	Gels progressively released a model molecule (fluorescein isothiocyanate-dextran) within 3-4 days with no initial burst release.
32724983	3	7	theme	pseudo	515:520	arg1	rotaxanes					522:530	poly(pseudo)rotaxanes	510:530	poly(pseudo)rotaxanes	510:530	The successful formation of poly(pseudo)rotaxanes and their supramolecular interactions were chemically demonstrated.
32724983	8	8	theme	gelation	1628:1635	arg1	time					1637:1640	longer gelation time	1621:1640	longer gelation time	1621:1640	At low PEU concentrations (1-5% w/v), cytocompatible gels (100% cell viability) were obtained, which maintained their shape in aqueous medium up to 5 d and completely dissolved within 7 d. PEU chemical composition affected PEU/α-cyclodextrin interactions, with longer gelation time and lower mechanical properties in gels based on PEU with pendant functionalities.
32724983	5	9	theme	%	884:884	arg1	w/v					886:888	9-10% w/v	880:888	9-10% w/v	880:888	Stability in physiological-like environment and mechanical properties improved with increasing α-cyclodextrin content (9-10% w/v), meanwhile gelation time decreased.
32724983	5	9	theme	%	884:884	arg1	content					871:877	α-cyclodextrin content	856:877	α-cyclodextrin content (9-10% w/v)	856:889	Stability in physiological-like environment and mechanical properties improved with increasing α-cyclodextrin content (9-10% w/v), meanwhile gelation time decreased.
32724983	6	10	theme	pseudo	956:961	arg1	crystals					973:980	poly(pseudo)rotaxanes crystals	951:980	poly(pseudo)rotaxanes crystals	951:980	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties was observed: the first were predominant at low PEU concentrations (1-5% w/v), while the latter prevailed at high PEU concentrations (7-9% w/v).
32724983	0	11	theme	delivery	104:111	arg1	characterization					134:149	characterization	134:149	characterization	134:149	Supramolecular hydrogels based on custom-made poly(ether urethane)s and cyclodextrins as potential drug delivery vehicles: design and characterization.
32724983	0	11	theme	delivery	104:111	arg1	design					123:128	design	123:128	design	123:128	Supramolecular hydrogels based on custom-made poly(ether urethane)s and cyclodextrins as potential drug delivery vehicles: design and characterization.
32724983	0	11	theme	delivery	104:111	arg1	vehicles					113:120	potential drug delivery vehicles	89:120	potential drug delivery vehicles: design and characterization	89:149	Supramolecular hydrogels based on custom-made poly(ether urethane)s and cyclodextrins as potential drug delivery vehicles: design and characterization.
32724983	3	12	theme	poly	510:513	arg1	rotaxanes					522:530	poly(pseudo)rotaxanes	510:530	poly(pseudo)rotaxanes	510:530	The successful formation of poly(pseudo)rotaxanes and their supramolecular interactions were chemically demonstrated.
32724983	6	13	theme	poly	951:954	arg1	crystals					973:980	poly(pseudo)rotaxanes crystals	951:980	poly(pseudo)rotaxanes crystals	951:980	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties was observed: the first were predominant at low PEU concentrations (1-5% w/v), while the latter prevailed at high PEU concentrations (7-9% w/v).
32724983	8	14	theme	PEU	1367:1369	arg1	w/v					1392:1394	1-5% w/v	1387:1394	1-5% w/v	1387:1394	At low PEU concentrations (1-5% w/v), cytocompatible gels (100% cell viability) were obtained, which maintained their shape in aqueous medium up to 5 d and completely dissolved within 7 d. PEU chemical composition affected PEU/α-cyclodextrin interactions, with longer gelation time and lower mechanical properties in gels based on PEU with pendant functionalities.
32724983	8	14	theme	PEU	1367:1369	arg1	concentrations					1371:1384	low PEU concentrations	1363:1384	low PEU concentrations (1-5% w/v)	1363:1395	At low PEU concentrations (1-5% w/v), cytocompatible gels (100% cell viability) were obtained, which maintained their shape in aqueous medium up to 5 d and completely dissolved within 7 d. PEU chemical composition affected PEU/α-cyclodextrin interactions, with longer gelation time and lower mechanical properties in gels based on PEU with pendant functionalities.
32724983	7	15	theme	aqueous	1329:1335	arg1	media					1337:1341	aqueous media	1329:1341	aqueous media improved	1329:1350	Increasing PEU concentration led to gels with increased dissolution rate, not-fully developed networks and slight cytotoxicity, meanwhile residence time in aqueous media improved (>7 d).
32724983	8	16	theme	mechanical	1652:1661	arg1	properties					1663:1672	lower mechanical properties	1646:1672	lower mechanical properties	1646:1672	At low PEU concentrations (1-5% w/v), cytocompatible gels (100% cell viability) were obtained, which maintained their shape in aqueous medium up to 5 d and completely dissolved within 7 d. PEU chemical composition affected PEU/α-cyclodextrin interactions, with longer gelation time and lower mechanical properties in gels based on PEU with pendant functionalities.
32724983	5	17	theme	α-cyclodextrin	856:869	arg1	time					911:914	meanwhile gelation time	892:914	meanwhile gelation time	892:914	Stability in physiological-like environment and mechanical properties improved with increasing α-cyclodextrin content (9-10% w/v), meanwhile gelation time decreased.
32724983	5	17	theme	α-cyclodextrin	856:869	arg1	w/v					886:888	9-10% w/v	880:888	9-10% w/v	880:888	Stability in physiological-like environment and mechanical properties improved with increasing α-cyclodextrin content (9-10% w/v), meanwhile gelation time decreased.
32724983	5	17	theme	α-cyclodextrin	856:869	arg1	content					871:877	α-cyclodextrin content	856:877	α-cyclodextrin content (9-10% w/v)	856:889	Stability in physiological-like environment and mechanical properties improved with increasing α-cyclodextrin content (9-10% w/v), meanwhile gelation time decreased.
32724983	10	18	theme	custom-made	1894:1904	arg1	PEUs					1906:1909	custom-made PEUs	1894:1909	custom-made PEUs	1894:1909	We thus demonstrated the suitability of custom-made PEUs as constituent of SM hydrogels with α-cyclodextrin and the high potential of the resulting systems for drug delivery applications.
32724983	3	19	theme	rotaxanes	522:530	arg1	formation					497:505	The successful formation	482:505	The successful formation of poly(pseudo)rotaxanes and their supramolecular interactions	482:568	The successful formation of poly(pseudo)rotaxanes and their supramolecular interactions were chemically demonstrated.
32724983	8	20	theme	%	1390:1390	arg1	w/v					1392:1394	1-5% w/v	1387:1394	1-5% w/v	1387:1394	At low PEU concentrations (1-5% w/v), cytocompatible gels (100% cell viability) were obtained, which maintained their shape in aqueous medium up to 5 d and completely dissolved within 7 d. PEU chemical composition affected PEU/α-cyclodextrin interactions, with longer gelation time and lower mechanical properties in gels based on PEU with pendant functionalities.
32724983	8	20	theme	%	1390:1390	arg1	concentrations					1371:1384	low PEU concentrations	1363:1384	low PEU concentrations (1-5% w/v)	1363:1395	At low PEU concentrations (1-5% w/v), cytocompatible gels (100% cell viability) were obtained, which maintained their shape in aqueous medium up to 5 d and completely dissolved within 7 d. PEU chemical composition affected PEU/α-cyclodextrin interactions, with longer gelation time and lower mechanical properties in gels based on PEU with pendant functionalities.
32724983	7	21	theme	meanwhile	1301:1309	arg1	cytotoxicity					1287:1298	slight cytotoxicity	1280:1298	slight cytotoxicity	1280:1298	Increasing PEU concentration led to gels with increased dissolution rate, not-fully developed networks and slight cytotoxicity, meanwhile residence time in aqueous media improved (>7 d).
32724983	7	21	theme	meanwhile	1301:1309	arg1	time					1321:1324	meanwhile residence time	1301:1324	meanwhile residence time in aqueous media improved	1301:1350	Increasing PEU concentration led to gels with increased dissolution rate, not-fully developed networks and slight cytotoxicity, meanwhile residence time in aqueous media improved (>7 d).
32724983	8	22	dep	PEU	1549:1551	arg1	affected					1574:1581	affected	1574:1581	affected	1574:1581	At low PEU concentrations (1-5% w/v), cytocompatible gels (100% cell viability) were obtained, which maintained their shape in aqueous medium up to 5 d and completely dissolved within 7 d. PEU chemical composition affected PEU/α-cyclodextrin interactions, with longer gelation time and lower mechanical properties in gels based on PEU with pendant functionalities.
32724983	10	23	theme	hydrogels	1932:1940	arg1	constituent					1914:1924	constituent	1914:1924	constituent of SM hydrogels with α-cyclodextrin and the high potential of the resulting systems for drug delivery applications	1914:2039	We thus demonstrated the suitability of custom-made PEUs as constituent of SM hydrogels with α-cyclodextrin and the high potential of the resulting systems for drug delivery applications.
32724983	10	24	theme	resulting	1992:2000	arg1	systems					2002:2008	the resulting systems	1988:2008	the resulting systems	1988:2008	We thus demonstrated the suitability of custom-made PEUs as constituent of SM hydrogels with α-cyclodextrin and the high potential of the resulting systems for drug delivery applications.
32724983	3	25	theme	interactions	557:568	arg1	formation					497:505	The successful formation	482:505	The successful formation of poly(pseudo)rotaxanes and their supramolecular interactions	482:568	The successful formation of poly(pseudo)rotaxanes and their supramolecular interactions were chemically demonstrated.
32724983	6	26	theme	micellar	990:997	arg1	structures					999:1008	PEU micellar structures	986:1008	PEU micellar structures	986:1008	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties was observed: the first were predominant at low PEU concentrations (1-5% w/v), while the latter prevailed at high PEU concentrations (7-9% w/v).
32724983	8	27	theme	cytocompatible	1398:1411	arg1	viability					1429:1437	100% cell viability	1419:1437	100% cell viability	1419:1437	At low PEU concentrations (1-5% w/v), cytocompatible gels (100% cell viability) were obtained, which maintained their shape in aqueous medium up to 5 d and completely dissolved within 7 d. PEU chemical composition affected PEU/α-cyclodextrin interactions, with longer gelation time and lower mechanical properties in gels based on PEU with pendant functionalities.
32724983	8	27	theme	cytocompatible	1398:1411	arg1	gels					1413:1416	cytocompatible gels	1398:1416	cytocompatible gels (100% cell viability)	1398:1438	At low PEU concentrations (1-5% w/v), cytocompatible gels (100% cell viability) were obtained, which maintained their shape in aqueous medium up to 5 d and completely dissolved within 7 d. PEU chemical composition affected PEU/α-cyclodextrin interactions, with longer gelation time and lower mechanical properties in gels based on PEU with pendant functionalities.
32724983	4	28	theme	supramolecular	649:662	arg1	hydrogels					664:672	self-healing (80-100% mechanical recovery) supramolecular hydrogels	606:672	self-healing (80-100% mechanical recovery) supramolecular hydrogels	606:672	Then, self-healing (80-100% mechanical recovery) supramolecular hydrogels were developed by mixing PEU and α-cyclodextrin solutions at different concentrations.
32724983	8	29	theme	pendant	1700:1706	arg1	functionalities					1708:1722	pendant functionalities	1700:1722	pendant functionalities	1700:1722	At low PEU concentrations (1-5% w/v), cytocompatible gels (100% cell viability) were obtained, which maintained their shape in aqueous medium up to 5 d and completely dissolved within 7 d. PEU chemical composition affected PEU/α-cyclodextrin interactions, with longer gelation time and lower mechanical properties in gels based on PEU with pendant functionalities.
32724983	5	30	theme	meanwhile	892:900	arg1	time					911:914	meanwhile gelation time	892:914	meanwhile gelation time	892:914	Stability in physiological-like environment and mechanical properties improved with increasing α-cyclodextrin content (9-10% w/v), meanwhile gelation time decreased.
32724983	5	30	theme	meanwhile	892:900	arg1	content					871:877	α-cyclodextrin content	856:877	α-cyclodextrin content (9-10% w/v)	856:889	Stability in physiological-like environment and mechanical properties improved with increasing α-cyclodextrin content (9-10% w/v), meanwhile gelation time decreased.
32724983	6	31	theme	rotaxanes	963:971	arg1	crystals					973:980	poly(pseudo)rotaxanes crystals	951:980	poly(pseudo)rotaxanes crystals	951:980	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties was observed: the first were predominant at low PEU concentrations (1-5% w/v), while the latter prevailed at high PEU concentrations (7-9% w/v).
32724983	8	32	theme	%	1422:1422	arg1	viability					1429:1437	100% cell viability	1419:1437	100% cell viability	1419:1437	At low PEU concentrations (1-5% w/v), cytocompatible gels (100% cell viability) were obtained, which maintained their shape in aqueous medium up to 5 d and completely dissolved within 7 d. PEU chemical composition affected PEU/α-cyclodextrin interactions, with longer gelation time and lower mechanical properties in gels based on PEU with pendant functionalities.
32724983	8	32	theme	%	1422:1422	arg1	gels					1413:1416	cytocompatible gels	1398:1416	cytocompatible gels (100% cell viability)	1398:1438	At low PEU concentrations (1-5% w/v), cytocompatible gels (100% cell viability) were obtained, which maintained their shape in aqueous medium up to 5 d and completely dissolved within 7 d. PEU chemical composition affected PEU/α-cyclodextrin interactions, with longer gelation time and lower mechanical properties in gels based on PEU with pendant functionalities.
32724983	2	33	theme	amphiphilic	376:386	arg1	PEUs					411:414	PEUs,	411:415	PEUs	411:414	In this work, we designed SM hydrogels based on α-cyclodextrin and high-molar mass amphiphilic poly(ether urethane)s (PEUs, ) based on Poloxamer® 407 and differing in their chain extender.
32724983	2	33	theme	amphiphilic	376:386	arg1	s					408:408	high-molar mass amphiphilic poly(ether urethane)s	360:408	high-molar mass amphiphilic poly(ether urethane)s (PEUs, )	360:417	In this work, we designed SM hydrogels based on α-cyclodextrin and high-molar mass amphiphilic poly(ether urethane)s (PEUs, ) based on Poloxamer® 407 and differing in their chain extender.
32724983	0	34	theme	potential	89:97	arg1	characterization					134:149	characterization	134:149	characterization	134:149	Supramolecular hydrogels based on custom-made poly(ether urethane)s and cyclodextrins as potential drug delivery vehicles: design and characterization.
32724983	0	34	theme	potential	89:97	arg1	design					123:128	design	123:128	design	123:128	Supramolecular hydrogels based on custom-made poly(ether urethane)s and cyclodextrins as potential drug delivery vehicles: design and characterization.
32724983	0	34	theme	potential	89:97	arg1	vehicles					113:120	potential drug delivery vehicles	89:120	potential drug delivery vehicles: design and characterization	89:149	Supramolecular hydrogels based on custom-made poly(ether urethane)s and cyclodextrins as potential drug delivery vehicles: design and characterization.
32724983	7	35	theme	slight	1280:1285	arg1	cytotoxicity					1287:1298	slight cytotoxicity	1280:1298	slight cytotoxicity	1280:1298	Increasing PEU concentration led to gels with increased dissolution rate, not-fully developed networks and slight cytotoxicity, meanwhile residence time in aqueous media improved (>7 d).
32724983	7	35	theme	slight	1280:1285	arg1	d					1356:1356	>7 d	1353:1356	>7 d	1353:1356	Increasing PEU concentration led to gels with increased dissolution rate, not-fully developed networks and slight cytotoxicity, meanwhile residence time in aqueous media improved (>7 d).
32724983	7	35	theme	slight	1280:1285	arg1	time					1321:1324	meanwhile residence time	1301:1324	meanwhile residence time in aqueous media improved	1301:1350	Increasing PEU concentration led to gels with increased dissolution rate, not-fully developed networks and slight cytotoxicity, meanwhile residence time in aqueous media improved (>7 d).
32724983	2	36	theme	high-molar	360:369	arg1	PEUs					411:414	PEUs,	411:415	PEUs	411:414	In this work, we designed SM hydrogels based on α-cyclodextrin and high-molar mass amphiphilic poly(ether urethane)s (PEUs, ) based on Poloxamer® 407 and differing in their chain extender.
32724983	2	36	theme	high-molar	360:369	arg1	s					408:408	high-molar mass amphiphilic poly(ether urethane)s	360:408	high-molar mass amphiphilic poly(ether urethane)s (PEUs, )	360:417	In this work, we designed SM hydrogels based on α-cyclodextrin and high-molar mass amphiphilic poly(ether urethane)s (PEUs, ) based on Poloxamer® 407 and differing in their chain extender.
32724983	0	37	theme	Supramolecular	0:13	arg1	hydrogels					15:23	Supramolecular hydrogels	0:23	Supramolecular hydrogels	0:23	Supramolecular hydrogels based on custom-made poly(ether urethane)s and cyclodextrins as potential drug delivery vehicles: design and characterization.
32724983	7	38	from	time	1321:1324	arg1	media					1337:1341	aqueous media	1329:1341	aqueous media improved	1329:1350	Increasing PEU concentration led to gels with increased dissolution rate, not-fully developed networks and slight cytotoxicity, meanwhile residence time in aqueous media improved (>7 d).
32724983	4	39	theme	α-cyclodextrin	707:720	arg1	solutions					722:730	α-cyclodextrin solutions	707:730	α-cyclodextrin solutions	707:730	Then, self-healing (80-100% mechanical recovery) supramolecular hydrogels were developed by mixing PEU and α-cyclodextrin solutions at different concentrations.
32724983	2	40	theme	urethane	399:406	arg1	PEUs					411:414	PEUs,	411:415	PEUs	411:414	In this work, we designed SM hydrogels based on α-cyclodextrin and high-molar mass amphiphilic poly(ether urethane)s (PEUs, ) based on Poloxamer® 407 and differing in their chain extender.
32724983	2	40	theme	urethane	399:406	arg1	s					408:408	high-molar mass amphiphilic poly(ether urethane)s	360:408	high-molar mass amphiphilic poly(ether urethane)s (PEUs, )	360:417	In this work, we designed SM hydrogels based on α-cyclodextrin and high-molar mass amphiphilic poly(ether urethane)s (PEUs, ) based on Poloxamer® 407 and differing in their chain extender.
32724983	1	41	theme	delivery	275:282	arg1	systems					284:290	drug delivery systems	270:290	drug delivery systems	270:290	The design of supramolecular (SM) hydrogels based on host-guest complexes represents an effective strategy to develop drug delivery systems.
32724983	0	42	theme	custom-made	34:44	arg1	poly					46:49	custom-made poly	34:49	custom-made poly(ether urethane)s	34:66	Supramolecular hydrogels based on custom-made poly(ether urethane)s and cyclodextrins as potential drug delivery vehicles: design and characterization.
32724983	0	42	theme	custom-made	34:44	arg1	urethane					57:64	ether urethane	51:64	ether urethane	51:64	Supramolecular hydrogels based on custom-made poly(ether urethane)s and cyclodextrins as potential drug delivery vehicles: design and characterization.
32724983	2	43	theme	SM	319:320	arg1	hydrogels					322:330	SM hydrogels	319:330	SM hydrogels based on α-cyclodextrin and high-molar mass amphiphilic poly(ether urethane)s (PEUs, ) based on Poloxamer® 407	319:441	In this work, we designed SM hydrogels based on α-cyclodextrin and high-molar mass amphiphilic poly(ether urethane)s (PEUs, ) based on Poloxamer® 407 and differing in their chain extender.
32724983	5	44	theme	physiological-like	774:791	arg1	environment					793:803	physiological-like environment	774:803	physiological-like environment	774:803	Stability in physiological-like environment and mechanical properties improved with increasing α-cyclodextrin content (9-10% w/v), meanwhile gelation time decreased.
32724983	6	45	theme	%	1099:1099	arg1	concentrations					1080:1093	low PEU concentrations	1072:1093	low PEU concentrations (1-5% w/v)	1072:1104	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties was observed: the first were predominant at low PEU concentrations (1-5% w/v), while the latter prevailed at high PEU concentrations (7-9% w/v).
32724983	6	45	theme	%	1099:1099	arg1	w/v					1101:1103	1-5% w/v	1096:1103	1-5% w/v	1096:1103	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties was observed: the first were predominant at low PEU concentrations (1-5% w/v), while the latter prevailed at high PEU concentrations (7-9% w/v).
32724983	9	46	theme	model	1755:1759	arg1	molecule					1761:1768	a model molecule	1753:1768	a model molecule (fluorescein isothiocyanate-dextran)	1753:1805	Gels progressively released a model molecule (fluorescein isothiocyanate-dextran) within 3-4 days with no initial burst release.
32724983	9	46	theme	model	1755:1759	arg1	isothiocyanate-dextran					1783:1804	fluorescein isothiocyanate-dextran	1771:1804	fluorescein isothiocyanate-dextran	1771:1804	Gels progressively released a model molecule (fluorescein isothiocyanate-dextran) within 3-4 days with no initial burst release.
32724983	8	47	theme	aqueous	1487:1493	arg1	medium					1495:1500	aqueous medium	1487:1500	aqueous medium up to 5 d	1487:1510	At low PEU concentrations (1-5% w/v), cytocompatible gels (100% cell viability) were obtained, which maintained their shape in aqueous medium up to 5 d and completely dissolved within 7 d. PEU chemical composition affected PEU/α-cyclodextrin interactions, with longer gelation time and lower mechanical properties in gels based on PEU with pendant functionalities.
32724983	8	48	theme	low	1363:1365	arg1	w/v					1392:1394	1-5% w/v	1387:1394	1-5% w/v	1387:1394	At low PEU concentrations (1-5% w/v), cytocompatible gels (100% cell viability) were obtained, which maintained their shape in aqueous medium up to 5 d and completely dissolved within 7 d. PEU chemical composition affected PEU/α-cyclodextrin interactions, with longer gelation time and lower mechanical properties in gels based on PEU with pendant functionalities.
32724983	8	48	theme	low	1363:1365	arg1	concentrations					1371:1384	low PEU concentrations	1363:1384	low PEU concentrations (1-5% w/v)	1363:1395	At low PEU concentrations (1-5% w/v), cytocompatible gels (100% cell viability) were obtained, which maintained their shape in aqueous medium up to 5 d and completely dissolved within 7 d. PEU chemical composition affected PEU/α-cyclodextrin interactions, with longer gelation time and lower mechanical properties in gels based on PEU with pendant functionalities.
32724983	8	49	from	properties	1663:1672	arg1	gels					1677:1680	gels	1677:1680	gels based on PEU with pendant functionalities	1677:1722	At low PEU concentrations (1-5% w/v), cytocompatible gels (100% cell viability) were obtained, which maintained their shape in aqueous medium up to 5 d and completely dissolved within 7 d. PEU chemical composition affected PEU/α-cyclodextrin interactions, with longer gelation time and lower mechanical properties in gels based on PEU with pendant functionalities.
32724983	4	50	dep	self-healing	606:617	arg1	%					626:626	80-100%	620:626	80-100% mechanical recovery	620:646	Then, self-healing (80-100% mechanical recovery) supramolecular hydrogels were developed by mixing PEU and α-cyclodextrin solutions at different concentrations.
32724983	6	51	theme	PEU	1076:1078	arg1	concentrations					1080:1093	low PEU concentrations	1072:1093	low PEU concentrations (1-5% w/v)	1072:1104	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties was observed: the first were predominant at low PEU concentrations (1-5% w/v), while the latter prevailed at high PEU concentrations (7-9% w/v).
32724983	6	51	theme	PEU	1076:1078	arg1	w/v					1101:1103	1-5% w/v	1096:1103	1-5% w/v	1096:1103	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties was observed: the first were predominant at low PEU concentrations (1-5% w/v), while the latter prevailed at high PEU concentrations (7-9% w/v).
32724983	10	52	theme	drug	2014:2017	arg1	applications					2028:2039	drug delivery applications	2014:2039	drug delivery applications	2014:2039	We thus demonstrated the suitability of custom-made PEUs as constituent of SM hydrogels with α-cyclodextrin and the high potential of the resulting systems for drug delivery applications.
32724983	7	53	theme	PEU	1184:1186	arg1	concentration					1188:1200	PEU concentration	1184:1200	PEU concentration	1184:1200	Increasing PEU concentration led to gels with increased dissolution rate, not-fully developed networks and slight cytotoxicity, meanwhile residence time in aqueous media improved (>7 d).
32724983	10	54	theme	systems	2002:2008	arg1	α-cyclodextrin					1947:1960	α-cyclodextrin	1947:1960	α-cyclodextrin	1947:1960	We thus demonstrated the suitability of custom-made PEUs as constituent of SM hydrogels with α-cyclodextrin and the high potential of the resulting systems for drug delivery applications.
32724983	10	54	theme	systems	2002:2008	arg1	potential					1975:1983	the high potential	1966:1983	the high potential of the resulting systems for drug delivery applications	1966:2039	We thus demonstrated the suitability of custom-made PEUs as constituent of SM hydrogels with α-cyclodextrin and the high potential of the resulting systems for drug delivery applications.
32724983	3	55	theme	successful	486:495	arg1	formation					497:505	The successful formation	482:505	The successful formation of poly(pseudo)rotaxanes and their supramolecular interactions	482:568	The successful formation of poly(pseudo)rotaxanes and their supramolecular interactions were chemically demonstrated.
32724983	10	56	theme	delivery	2019:2026	arg1	applications					2028:2039	drug delivery applications	2014:2039	drug delivery applications	2014:2039	We thus demonstrated the suitability of custom-made PEUs as constituent of SM hydrogels with α-cyclodextrin and the high potential of the resulting systems for drug delivery applications.
32724983	6	57	attach	predominant	1057:1067	arg2	predominant					1057:1067	predominant	1057:1067	predominant	1057:1067	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties was observed: the first were predominant at low PEU concentrations (1-5% w/v), while the latter prevailed at high PEU concentrations (7-9% w/v).
32724983	6	57	attach	predominant	1057:1067	arg2	first					1046:1050	first	1046:1050	first	1046:1050	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties was observed: the first were predominant at low PEU concentrations (1-5% w/v), while the latter prevailed at high PEU concentrations (7-9% w/v).
32724983	6	57	attach	predominant	1057:1067	arg1	concentrations					1080:1093	low PEU concentrations	1072:1093	low PEU concentrations (1-5% w/v)	1072:1104	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties was observed: the first were predominant at low PEU concentrations (1-5% w/v), while the latter prevailed at high PEU concentrations (7-9% w/v).
32724983	6	57	attach	predominant	1057:1067	arg1	w/v					1101:1103	1-5% w/v	1096:1103	1-5% w/v	1096:1103	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties was observed: the first were predominant at low PEU concentrations (1-5% w/v), while the latter prevailed at high PEU concentrations (7-9% w/v).
32724983	8	58	theme	PEU/α-cyclodextrin	1583:1600	arg1	interactions					1602:1613	PEU/α-cyclodextrin interactions	1583:1613	PEU/α-cyclodextrin interactions	1583:1613	At low PEU concentrations (1-5% w/v), cytocompatible gels (100% cell viability) were obtained, which maintained their shape in aqueous medium up to 5 d and completely dissolved within 7 d. PEU chemical composition affected PEU/α-cyclodextrin interactions, with longer gelation time and lower mechanical properties in gels based on PEU with pendant functionalities.
32724983	4	59	theme	self-healing	606:617	arg1	hydrogels					664:672	self-healing (80-100% mechanical recovery) supramolecular hydrogels	606:672	self-healing (80-100% mechanical recovery) supramolecular hydrogels	606:672	Then, self-healing (80-100% mechanical recovery) supramolecular hydrogels were developed by mixing PEU and α-cyclodextrin solutions at different concentrations.
32724983	1	60	theme	supramolecular	166:179	arg1	hydrogels					186:194	supramolecular (SM) hydrogels	166:194	supramolecular (SM) hydrogels based on host-guest complexes	166:224	The design of supramolecular (SM) hydrogels based on host-guest complexes represents an effective strategy to develop drug delivery systems.
32724983	9	61	theme	burst	1839:1843	arg1	release					1845:1851	no initial burst release	1828:1851	no initial burst release	1828:1851	Gels progressively released a model molecule (fluorescein isothiocyanate-dextran) within 3-4 days with no initial burst release.
32724983	8	62	theme	longer	1621:1626	arg1	time					1637:1640	longer gelation time	1621:1640	longer gelation time	1621:1640	At low PEU concentrations (1-5% w/v), cytocompatible gels (100% cell viability) were obtained, which maintained their shape in aqueous medium up to 5 d and completely dissolved within 7 d. PEU chemical composition affected PEU/α-cyclodextrin interactions, with longer gelation time and lower mechanical properties in gels based on PEU with pendant functionalities.
32724983	1	63	theme	SM	182:183	arg1	hydrogels					186:194	supramolecular (SM) hydrogels	166:194	supramolecular (SM) hydrogels based on host-guest complexes	166:224	The design of supramolecular (SM) hydrogels based on host-guest complexes represents an effective strategy to develop drug delivery systems.
32724983	8	64	with	PEU	1691:1693	arg1	functionalities					1708:1722	pendant functionalities	1700:1722	pendant functionalities	1700:1722	At low PEU concentrations (1-5% w/v), cytocompatible gels (100% cell viability) were obtained, which maintained their shape in aqueous medium up to 5 d and completely dissolved within 7 d. PEU chemical composition affected PEU/α-cyclodextrin interactions, with longer gelation time and lower mechanical properties in gels based on PEU with pendant functionalities.
32724983	7	65	theme	increased	1219:1227	arg1	rate					1241:1244	increased dissolution rate	1219:1244	increased dissolution rate	1219:1244	Increasing PEU concentration led to gels with increased dissolution rate, not-fully developed networks and slight cytotoxicity, meanwhile residence time in aqueous media improved (>7 d).
32724983	1	66	theme	hydrogels	186:194	arg1	design					156:161	The design	152:161	The design of supramolecular (SM) hydrogels based on host-guest complexes	152:224	The design of supramolecular (SM) hydrogels based on host-guest complexes represents an effective strategy to develop drug delivery systems.
32724983	10	67	theme	PEUs	1906:1909	arg1	suitability					1879:1889	the suitability	1875:1889	the suitability of custom-made PEUs as constituent of SM hydrogels with α-cyclodextrin and the high potential of the resulting systems for drug delivery applications	1875:2039	We thus demonstrated the suitability of custom-made PEUs as constituent of SM hydrogels with α-cyclodextrin and the high potential of the resulting systems for drug delivery applications.
32724983	8	68	theme	lower	1646:1650	arg1	properties					1663:1672	lower mechanical properties	1646:1672	lower mechanical properties	1646:1672	At low PEU concentrations (1-5% w/v), cytocompatible gels (100% cell viability) were obtained, which maintained their shape in aqueous medium up to 5 d and completely dissolved within 7 d. PEU chemical composition affected PEU/α-cyclodextrin interactions, with longer gelation time and lower mechanical properties in gels based on PEU with pendant functionalities.
32724983	5	69	theme	gelation	902:909	arg1	time					911:914	meanwhile gelation time	892:914	meanwhile gelation time	892:914	Stability in physiological-like environment and mechanical properties improved with increasing α-cyclodextrin content (9-10% w/v), meanwhile gelation time decreased.
32724983	5	69	theme	gelation	902:909	arg1	content					871:877	α-cyclodextrin content	856:877	α-cyclodextrin content (9-10% w/v)	856:889	Stability in physiological-like environment and mechanical properties improved with increasing α-cyclodextrin content (9-10% w/v), meanwhile gelation time decreased.
32724983	6	70	theme	synergistic	929:939	arg1	effect					941:946	A synergistic effect	927:946	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties	927:1026	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties was observed: the first were predominant at low PEU concentrations (1-5% w/v), while the latter prevailed at high PEU concentrations (7-9% w/v).
32724983	6	71	theme	%	1165:1165	arg1	w/v					1167:1169	7-9% w/v	1162:1169	7-9% w/v	1162:1169	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties was observed: the first were predominant at low PEU concentrations (1-5% w/v), while the latter prevailed at high PEU concentrations (7-9% w/v).
32724983	6	71	theme	%	1165:1165	arg1	concentrations					1146:1159	high PEU concentrations	1137:1159	high PEU concentrations (7-9% w/v)	1137:1170	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties was observed: the first were predominant at low PEU concentrations (1-5% w/v), while the latter prevailed at high PEU concentrations (7-9% w/v).
32724983	6	72	theme	gel	1013:1015	arg1	properties					1017:1026	gel properties	1013:1026	gel properties	1013:1026	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties was observed: the first were predominant at low PEU concentrations (1-5% w/v), while the latter prevailed at high PEU concentrations (7-9% w/v).
32724983	7	73	theme	residence	1311:1319	arg1	cytotoxicity					1287:1298	slight cytotoxicity	1280:1298	slight cytotoxicity	1280:1298	Increasing PEU concentration led to gels with increased dissolution rate, not-fully developed networks and slight cytotoxicity, meanwhile residence time in aqueous media improved (>7 d).
32724983	7	73	theme	residence	1311:1319	arg1	time					1321:1324	meanwhile residence time	1301:1324	meanwhile residence time in aqueous media improved	1301:1350	Increasing PEU concentration led to gels with increased dissolution rate, not-fully developed networks and slight cytotoxicity, meanwhile residence time in aqueous media improved (>7 d).
32724983	10	74	theme	SM	1929:1930	arg1	hydrogels					1932:1940	SM hydrogels	1929:1940	SM hydrogels with α-cyclodextrin and the high potential of the resulting systems for drug delivery applications	1929:2039	We thus demonstrated the suitability of custom-made PEUs as constituent of SM hydrogels with α-cyclodextrin and the high potential of the resulting systems for drug delivery applications.
32724983	3	75	theme	supramolecular	542:555	arg1	interactions					557:568	their supramolecular interactions	536:568	their supramolecular interactions	536:568	The successful formation of poly(pseudo)rotaxanes and their supramolecular interactions were chemically demonstrated.
32724983	6	76	theme	structures	999:1008	arg1	effect					941:946	A synergistic effect	927:946	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties	927:1026	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties was observed: the first were predominant at low PEU concentrations (1-5% w/v), while the latter prevailed at high PEU concentrations (7-9% w/v).
32724983	6	77	theme	PEU	1142:1144	arg1	w/v					1167:1169	7-9% w/v	1162:1169	7-9% w/v	1162:1169	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties was observed: the first were predominant at low PEU concentrations (1-5% w/v), while the latter prevailed at high PEU concentrations (7-9% w/v).
32724983	6	77	theme	PEU	1142:1144	arg1	concentrations					1146:1159	high PEU concentrations	1137:1159	high PEU concentrations (7-9% w/v)	1137:1170	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties was observed: the first were predominant at low PEU concentrations (1-5% w/v), while the latter prevailed at high PEU concentrations (7-9% w/v).
32724983	4	78	theme	mechanical	628:637	arg1	recovery					639:646	mechanical recovery	628:646	80-100% mechanical recovery	620:646	Then, self-healing (80-100% mechanical recovery) supramolecular hydrogels were developed by mixing PEU and α-cyclodextrin solutions at different concentrations.
32724983	0	79	dep	vehicles	113:120	arg1	characterization					134:149	characterization	134:149	characterization	134:149	Supramolecular hydrogels based on custom-made poly(ether urethane)s and cyclodextrins as potential drug delivery vehicles: design and characterization.
32724983	0	79	dep	vehicles	113:120	arg1	design					123:128	design	123:128	design	123:128	Supramolecular hydrogels based on custom-made poly(ether urethane)s and cyclodextrins as potential drug delivery vehicles: design and characterization.
32724983	0	79	dep	vehicles	113:120	arg1	vehicles					113:120	potential drug delivery vehicles	89:120	potential drug delivery vehicles: design and characterization	89:149	Supramolecular hydrogels based on custom-made poly(ether urethane)s and cyclodextrins as potential drug delivery vehicles: design and characterization.
32724983	6	80	theme	PEU	986:988	arg1	structures					999:1008	PEU micellar structures	986:1008	PEU micellar structures	986:1008	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties was observed: the first were predominant at low PEU concentrations (1-5% w/v), while the latter prevailed at high PEU concentrations (7-9% w/v).
32724983	8	81	theme	100	1419:1421	arg1	%					1422:1422	%	1422:1422	%	1422:1422	At low PEU concentrations (1-5% w/v), cytocompatible gels (100% cell viability) were obtained, which maintained their shape in aqueous medium up to 5 d and completely dissolved within 7 d. PEU chemical composition affected PEU/α-cyclodextrin interactions, with longer gelation time and lower mechanical properties in gels based on PEU with pendant functionalities.
32724983	5	82	from	Stability	761:769	arg1	environment					793:803	physiological-like environment	774:803	physiological-like environment	774:803	Stability in physiological-like environment and mechanical properties improved with increasing α-cyclodextrin content (9-10% w/v), meanwhile gelation time decreased.
32724983	6	83	theme	crystals	973:980	arg1	effect					941:946	A synergistic effect	927:946	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties	927:1026	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties was observed: the first were predominant at low PEU concentrations (1-5% w/v), while the latter prevailed at high PEU concentrations (7-9% w/v).
32724983	2	84	theme	chain	466:470	arg1	extender					472:479	their chain extender	460:479	their chain extender	460:479	In this work, we designed SM hydrogels based on α-cyclodextrin and high-molar mass amphiphilic poly(ether urethane)s (PEUs, ) based on Poloxamer® 407 and differing in their chain extender.
32724983	8	85	theme	cell	1424:1427	arg1	viability					1429:1437	100% cell viability	1419:1437	100% cell viability	1419:1437	At low PEU concentrations (1-5% w/v), cytocompatible gels (100% cell viability) were obtained, which maintained their shape in aqueous medium up to 5 d and completely dissolved within 7 d. PEU chemical composition affected PEU/α-cyclodextrin interactions, with longer gelation time and lower mechanical properties in gels based on PEU with pendant functionalities.
32724983	8	85	theme	cell	1424:1427	arg1	gels					1413:1416	cytocompatible gels	1398:1416	cytocompatible gels (100% cell viability)	1398:1438	At low PEU concentrations (1-5% w/v), cytocompatible gels (100% cell viability) were obtained, which maintained their shape in aqueous medium up to 5 d and completely dissolved within 7 d. PEU chemical composition affected PEU/α-cyclodextrin interactions, with longer gelation time and lower mechanical properties in gels based on PEU with pendant functionalities.
32724983	0	86	theme	drug	99:102	arg1	characterization					134:149	characterization	134:149	characterization	134:149	Supramolecular hydrogels based on custom-made poly(ether urethane)s and cyclodextrins as potential drug delivery vehicles: design and characterization.
32724983	0	86	theme	drug	99:102	arg1	design					123:128	design	123:128	design	123:128	Supramolecular hydrogels based on custom-made poly(ether urethane)s and cyclodextrins as potential drug delivery vehicles: design and characterization.
32724983	0	86	theme	drug	99:102	arg1	vehicles					113:120	potential drug delivery vehicles	89:120	potential drug delivery vehicles: design and characterization	89:149	Supramolecular hydrogels based on custom-made poly(ether urethane)s and cyclodextrins as potential drug delivery vehicles: design and characterization.
32724983	5	87	from	properties	820:829	arg1	environment					793:803	physiological-like environment	774:803	physiological-like environment	774:803	Stability in physiological-like environment and mechanical properties improved with increasing α-cyclodextrin content (9-10% w/v), meanwhile gelation time decreased.
32724983	4	88	theme	different	735:743	arg1	concentrations					745:758	different concentrations	735:758	different concentrations	735:758	Then, self-healing (80-100% mechanical recovery) supramolecular hydrogels were developed by mixing PEU and α-cyclodextrin solutions at different concentrations.
32724983	2	89	theme	mass	371:374	arg1	PEUs					411:414	PEUs,	411:415	PEUs	411:414	In this work, we designed SM hydrogels based on α-cyclodextrin and high-molar mass amphiphilic poly(ether urethane)s (PEUs, ) based on Poloxamer® 407 and differing in their chain extender.
32724983	2	89	theme	mass	371:374	arg1	s					408:408	high-molar mass amphiphilic poly(ether urethane)s	360:408	high-molar mass amphiphilic poly(ether urethane)s (PEUs, )	360:417	In this work, we designed SM hydrogels based on α-cyclodextrin and high-molar mass amphiphilic poly(ether urethane)s (PEUs, ) based on Poloxamer® 407 and differing in their chain extender.
32724983	7	90	theme	developed	1257:1265	arg1	networks					1267:1274	not-fully developed networks	1247:1274	not-fully developed networks	1247:1274	Increasing PEU concentration led to gels with increased dissolution rate, not-fully developed networks and slight cytotoxicity, meanwhile residence time in aqueous media improved (>7 d).
32724983	5	91	theme	mechanical	809:818	arg1	properties					820:829	mechanical properties	809:829	mechanical properties improved with increasing α-cyclodextrin content (9-10% w/v), meanwhile gelation time	809:914	Stability in physiological-like environment and mechanical properties improved with increasing α-cyclodextrin content (9-10% w/v), meanwhile gelation time decreased.
32724983	1	92	theme	drug	270:273	arg1	systems					284:290	drug delivery systems	270:290	drug delivery systems	270:290	The design of supramolecular (SM) hydrogels based on host-guest complexes represents an effective strategy to develop drug delivery systems.
32724983	2	93	theme	ether	393:397	arg1	PEUs					411:414	PEUs,	411:415	PEUs	411:414	In this work, we designed SM hydrogels based on α-cyclodextrin and high-molar mass amphiphilic poly(ether urethane)s (PEUs, ) based on Poloxamer® 407 and differing in their chain extender.
32724983	2	93	theme	ether	393:397	arg1	s					408:408	high-molar mass amphiphilic poly(ether urethane)s	360:408	high-molar mass amphiphilic poly(ether urethane)s (PEUs, )	360:417	In this work, we designed SM hydrogels based on α-cyclodextrin and high-molar mass amphiphilic poly(ether urethane)s (PEUs, ) based on Poloxamer® 407 and differing in their chain extender.
32724983	0	94	theme	poly	46:49	arg1	s					66:66	custom-made poly(ether urethane)s	34:66	custom-made poly(ether urethane)s	34:66	Supramolecular hydrogels based on custom-made poly(ether urethane)s and cyclodextrins as potential drug delivery vehicles: design and characterization.
32724983	10	95	theme	high	1970:1973	arg1	potential					1975:1983	the high potential	1966:1983	the high potential of the resulting systems for drug delivery applications	1966:2039	We thus demonstrated the suitability of custom-made PEUs as constituent of SM hydrogels with α-cyclodextrin and the high potential of the resulting systems for drug delivery applications.
32724983	7	96	theme	dissolution	1229:1239	arg1	rate					1241:1244	increased dissolution rate	1219:1244	increased dissolution rate	1219:1244	Increasing PEU concentration led to gels with increased dissolution rate, not-fully developed networks and slight cytotoxicity, meanwhile residence time in aqueous media improved (>7 d).
32724983	2	97	theme	poly	388:391	arg1	PEUs					411:414	PEUs,	411:415	PEUs	411:414	In this work, we designed SM hydrogels based on α-cyclodextrin and high-molar mass amphiphilic poly(ether urethane)s (PEUs, ) based on Poloxamer® 407 and differing in their chain extender.
32724983	2	97	theme	poly	388:391	arg1	s					408:408	high-molar mass amphiphilic poly(ether urethane)s	360:408	high-molar mass amphiphilic poly(ether urethane)s (PEUs, )	360:417	In this work, we designed SM hydrogels based on α-cyclodextrin and high-molar mass amphiphilic poly(ether urethane)s (PEUs, ) based on Poloxamer® 407 and differing in their chain extender.
32724983	0	98	theme	ether	51:55	arg1	poly					46:49	custom-made poly	34:49	custom-made poly(ether urethane)s	34:66	Supramolecular hydrogels based on custom-made poly(ether urethane)s and cyclodextrins as potential drug delivery vehicles: design and characterization.
32724983	0	98	theme	ether	51:55	arg1	urethane					57:64	ether urethane	51:64	ether urethane	51:64	Supramolecular hydrogels based on custom-made poly(ether urethane)s and cyclodextrins as potential drug delivery vehicles: design and characterization.
32724983	4	99	dep	%	626:626	arg1	recovery					639:646	mechanical recovery	628:646	80-100% mechanical recovery	620:646	Then, self-healing (80-100% mechanical recovery) supramolecular hydrogels were developed by mixing PEU and α-cyclodextrin solutions at different concentrations.
32724983	6	100	from	effect	941:946	arg1	properties					1017:1026	gel properties	1013:1026	gel properties	1013:1026	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties was observed: the first were predominant at low PEU concentrations (1-5% w/v), while the latter prevailed at high PEU concentrations (7-9% w/v).
32724983	9	101	with	days	1818:1821	arg1	release					1845:1851	no initial burst release	1828:1851	no initial burst release	1828:1851	Gels progressively released a model molecule (fluorescein isothiocyanate-dextran) within 3-4 days with no initial burst release.
32724983	8	102	from	time	1637:1640	arg1	gels					1677:1680	gels	1677:1680	gels based on PEU with pendant functionalities	1677:1722	At low PEU concentrations (1-5% w/v), cytocompatible gels (100% cell viability) were obtained, which maintained their shape in aqueous medium up to 5 d and completely dissolved within 7 d. PEU chemical composition affected PEU/α-cyclodextrin interactions, with longer gelation time and lower mechanical properties in gels based on PEU with pendant functionalities.
32724983	1	103	theme	host-guest	205:214	arg1	complexes					216:224	host-guest complexes	205:224	host-guest complexes	205:224	The design of supramolecular (SM) hydrogels based on host-guest complexes represents an effective strategy to develop drug delivery systems.
32724983	9	104	theme	fluorescein	1771:1781	arg1	molecule					1761:1768	a model molecule	1753:1768	a model molecule (fluorescein isothiocyanate-dextran)	1753:1805	Gels progressively released a model molecule (fluorescein isothiocyanate-dextran) within 3-4 days with no initial burst release.
32724983	9	104	theme	fluorescein	1771:1781	arg1	isothiocyanate-dextran					1783:1804	fluorescein isothiocyanate-dextran	1771:1804	fluorescein isothiocyanate-dextran	1771:1804	Gels progressively released a model molecule (fluorescein isothiocyanate-dextran) within 3-4 days with no initial burst release.
32724983	6	105	from	concentrations	1080:1093	arg1	first					1046:1050	first	1046:1050	first	1046:1050	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties was observed: the first were predominant at low PEU concentrations (1-5% w/v), while the latter prevailed at high PEU concentrations (7-9% w/v).
32724983	6	105	from	concentrations	1080:1093	arg1	predominant					1057:1067	predominant	1057:1067	predominant	1057:1067	A synergistic effect of poly(pseudo)rotaxanes crystals and PEU micellar structures on gel properties was observed: the first were predominant at low PEU concentrations (1-5% w/v), while the latter prevailed at high PEU concentrations (7-9% w/v).
32724983	8	106	theme	chemical	1553:1560	arg1	composition					1562:1572	chemical composition	1553:1572	chemical composition	1553:1572	At low PEU concentrations (1-5% w/v), cytocompatible gels (100% cell viability) were obtained, which maintained their shape in aqueous medium up to 5 d and completely dissolved within 7 d. PEU chemical composition affected PEU/α-cyclodextrin interactions, with longer gelation time and lower mechanical properties in gels based on PEU with pendant functionalities.
34236956	2	0	theme	mixotrophic	112:122	arg1	140T					161:164	A mixotrophic and acidophilic bacterial strain BGR 140T	110:164	A mixotrophic and acidophilic bacterial strain BGR 140T	110:164	A mixotrophic and acidophilic bacterial strain BGR 140T was isolated from mine tailings in the Harz Mountains near Goslar, Germany.
34236956	11	1	theme	Sulfobacillus	1216:1228	arg1	species					1195:1201	a novel species	1187:1201	a novel species	1187:1201	On the basis of the results of phenotypic and genomic analyses, it is concluded that strain BGR 140T represents a novel species of the genus Sulfobacillus, for which the name Sulfobacillus harzensis sp.
34236956	5	2	dep	Sulfobacillus	689:701	arg1	benefaciens					703:713	benefaciens	703:713	benefaciens	703:713	The results of analysis of the 16S rRNA gene sequences indicated that BGR 140T was phylogenetically related to different members of the genus Sulfobacillus, and the sequence identities to Sulfobacillus acidophilus DSM 10332T, Sulfobacillus thermotolerans DSM 17362T, and Sulfobacillus benefaciens DSM 19468T were 94.8, 91.8 and 91.6 %, respectively.
34236956	11	3	theme	novel	1189:1193	arg1	species					1195:1201	a novel species	1187:1201	a novel species	1187:1201	On the basis of the results of phenotypic and genomic analyses, it is concluded that strain BGR 140T represents a novel species of the genus Sulfobacillus, for which the name Sulfobacillus harzensis sp.
34236956	10	4	theme	G+C	1049:1051	arg1	content					1053:1059	The genomic DNA G+C content	1033:1059	The genomic DNA G+C content	1033:1059	The genomic DNA G+C content is 58.2 mol%.
34236956	10	4	theme	G+C	1049:1051	arg1	%					1072:1072	58.2 mol%	1064:1072	58.2 mol%	1064:1072	The genomic DNA G+C content is 58.2 mol%.
34236956	6	5	theme	wall	777:780	arg1	peptidoglycan					782:794	Its cell wall peptidoglycan	768:794	Its cell wall peptidoglycan	768:794	Its cell wall peptidoglycan is A1γ, composed of meso-diaminopimelic acid.
34236956	13	6	theme	=DSM	1347:1350	arg1	39070T					1364:1369	=DSM 109850T=JCM 39070T	1347:1369	=DSM 109850T=JCM 39070T	1347:1369	Its type strain is BGR 140T (=DSM 109850T=JCM 39070T).
34236956	13	6	theme	=DSM	1347:1350	arg1	140T					1341:1344	BGR 140T	1337:1344	BGR 140T (=DSM 109850T=JCM 39070T)	1337:1370	Its type strain is BGR 140T (=DSM 109850T=JCM 39070T).
34236956	11	7	dep	Sulfobacillus	1250:1262	arg1	harzensis					1264:1272	harzensis	1264:1272	harzensis	1264:1272	On the basis of the results of phenotypic and genomic analyses, it is concluded that strain BGR 140T represents a novel species of the genus Sulfobacillus, for which the name Sulfobacillus harzensis sp.
34236956	2	8	from	tailings	189:196	arg1	Mountains					210:218	Mountains	210:218	Mountains	210:218	A mixotrophic and acidophilic bacterial strain BGR 140T was isolated from mine tailings in the Harz Mountains near Goslar, Germany.
34236956	6	9	theme	cell	772:775	arg1	peptidoglycan					782:794	Its cell wall peptidoglycan	768:794	Its cell wall peptidoglycan	768:794	Its cell wall peptidoglycan is A1γ, composed of meso-diaminopimelic acid.
34236956	5	10	theme	Sulfobacillus	606:618	arg1	10332T					636:641	Sulfobacillus acidophilus DSM 10332T	606:641	Sulfobacillus acidophilus DSM 10332T	606:641	The results of analysis of the 16S rRNA gene sequences indicated that BGR 140T was phylogenetically related to different members of the genus Sulfobacillus, and the sequence identities to Sulfobacillus acidophilus DSM 10332T, Sulfobacillus thermotolerans DSM 17362T, and Sulfobacillus benefaciens DSM 19468T were 94.8, 91.8 and 91.6 %, respectively.
34236956	11	11	theme	analyses	1129:1136	arg1	results					1095:1101	the results	1091:1101	the results of phenotypic and genomic analyses	1091:1136	On the basis of the results of phenotypic and genomic analyses, it is concluded that strain BGR 140T represents a novel species of the genus Sulfobacillus, for which the name Sulfobacillus harzensis sp.
34236956	5	12	theme	Sulfobacillus	689:701	arg1	19468T					719:724	Sulfobacillus benefaciens DSM 19468T	689:724	Sulfobacillus benefaciens DSM 19468T	689:724	The results of analysis of the 16S rRNA gene sequences indicated that BGR 140T was phylogenetically related to different members of the genus Sulfobacillus, and the sequence identities to Sulfobacillus acidophilus DSM 10332T, Sulfobacillus thermotolerans DSM 17362T, and Sulfobacillus benefaciens DSM 19468T were 94.8, 91.8 and 91.6 %, respectively.
34236956	2	13	theme	BGR	157:159	arg1	140T					161:164	A mixotrophic and acidophilic bacterial strain BGR 140T	110:164	A mixotrophic and acidophilic bacterial strain BGR 140T	110:164	A mixotrophic and acidophilic bacterial strain BGR 140T was isolated from mine tailings in the Harz Mountains near Goslar, Germany.
34236956	11	14	theme	genus	1210:1214	arg1	Sulfobacillus					1216:1228	the genus Sulfobacillus	1206:1228	the genus Sulfobacillus	1206:1228	On the basis of the results of phenotypic and genomic analyses, it is concluded that strain BGR 140T represents a novel species of the genus Sulfobacillus, for which the name Sulfobacillus harzensis sp.
34236956	5	15	theme	genus	554:558	arg1	Sulfobacillus					560:572	the genus Sulfobacillus	550:572	the genus Sulfobacillus	550:572	The results of analysis of the 16S rRNA gene sequences indicated that BGR 140T was phylogenetically related to different members of the genus Sulfobacillus, and the sequence identities to Sulfobacillus acidophilus DSM 10332T, Sulfobacillus thermotolerans DSM 17362T, and Sulfobacillus benefaciens DSM 19468T were 94.8, 91.8 and 91.6 %, respectively.
34236956	2	16	theme	strain	150:155	arg1	140T					161:164	A mixotrophic and acidophilic bacterial strain BGR 140T	110:164	A mixotrophic and acidophilic bacterial strain BGR 140T	110:164	A mixotrophic and acidophilic bacterial strain BGR 140T was isolated from mine tailings in the Harz Mountains near Goslar, Germany.
34236956	5	17	theme	Sulfobacillus	560:572	arg1	members					539:545	different members	529:545	different members of the genus Sulfobacillus	529:572	The results of analysis of the 16S rRNA gene sequences indicated that BGR 140T was phylogenetically related to different members of the genus Sulfobacillus, and the sequence identities to Sulfobacillus acidophilus DSM 10332T, Sulfobacillus thermotolerans DSM 17362T, and Sulfobacillus benefaciens DSM 19468T were 94.8, 91.8 and 91.6 %, respectively.
34236956	1	18	theme	mine	70:73	arg1	tailings					75:82	mine tailings	70:82	mine tailings	70:82	nov., an acidophilic bacterium inhabiting mine tailings from a polymetallic mine.
34236956	5	19	theme	DSM	715:717	arg1	19468T					719:724	Sulfobacillus benefaciens DSM 19468T	689:724	Sulfobacillus benefaciens DSM 19468T	689:724	The results of analysis of the 16S rRNA gene sequences indicated that BGR 140T was phylogenetically related to different members of the genus Sulfobacillus, and the sequence identities to Sulfobacillus acidophilus DSM 10332T, Sulfobacillus thermotolerans DSM 17362T, and Sulfobacillus benefaciens DSM 19468T were 94.8, 91.8 and 91.6 %, respectively.
34236956	2	20	theme	bacterial	140:148	arg1	140T					161:164	A mixotrophic and acidophilic bacterial strain BGR 140T	110:164	A mixotrophic and acidophilic bacterial strain BGR 140T	110:164	A mixotrophic and acidophilic bacterial strain BGR 140T was isolated from mine tailings in the Harz Mountains near Goslar, Germany.
34236956	11	21	theme	name	1245:1248	arg1	sp					1274:1275	the name Sulfobacillus harzensis sp	1241:1275	the name Sulfobacillus harzensis sp	1241:1275	On the basis of the results of phenotypic and genomic analyses, it is concluded that strain BGR 140T represents a novel species of the genus Sulfobacillus, for which the name Sulfobacillus harzensis sp.
34236956	11	22	theme	genomic	1121:1127	arg1	analyses					1129:1136	phenotypic and genomic analyses	1106:1136	phenotypic and genomic analyses	1106:1136	On the basis of the results of phenotypic and genomic analyses, it is concluded that strain BGR 140T represents a novel species of the genus Sulfobacillus, for which the name Sulfobacillus harzensis sp.
34236956	5	23	theme	16S	449:451	arg1	sequences					463:471	the 16S rRNA gene sequences	445:471	the 16S rRNA gene sequences	445:471	The results of analysis of the 16S rRNA gene sequences indicated that BGR 140T was phylogenetically related to different members of the genus Sulfobacillus, and the sequence identities to Sulfobacillus acidophilus DSM 10332T, Sulfobacillus thermotolerans DSM 17362T, and Sulfobacillus benefaciens DSM 19468T were 94.8, 91.8 and 91.6 %, respectively.
34236956	0	24	theme	harzensis	14:22	arg1	sp					24:25	Sulfobacillus harzensis sp	0:25	Sulfobacillus harzensis sp.	0:26	Sulfobacillus harzensis sp.
34236956	2	25	theme	acidophilic	128:138	arg1	140T					161:164	A mixotrophic and acidophilic bacterial strain BGR 140T	110:164	A mixotrophic and acidophilic bacterial strain BGR 140T	110:164	A mixotrophic and acidophilic bacterial strain BGR 140T was isolated from mine tailings in the Harz Mountains near Goslar, Germany.
34236956	5	26	theme	rRNA	453:456	arg1	sequences					463:471	the 16S rRNA gene sequences	445:471	the 16S rRNA gene sequences	445:471	The results of analysis of the 16S rRNA gene sequences indicated that BGR 140T was phylogenetically related to different members of the genus Sulfobacillus, and the sequence identities to Sulfobacillus acidophilus DSM 10332T, Sulfobacillus thermotolerans DSM 17362T, and Sulfobacillus benefaciens DSM 19468T were 94.8, 91.8 and 91.6 %, respectively.
34236956	0	27	theme	Sulfobacillus	0:12	arg1	sp					24:25	Sulfobacillus harzensis sp	0:25	Sulfobacillus harzensis sp.	0:26	Sulfobacillus harzensis sp.
34236956	5	28	theme	sequence	583:590	arg1	%					751:751	94.8, 91.8 and 91.6 %	731:751	94.8, 91.8 and 91.6 %	731:751	The results of analysis of the 16S rRNA gene sequences indicated that BGR 140T was phylogenetically related to different members of the genus Sulfobacillus, and the sequence identities to Sulfobacillus acidophilus DSM 10332T, Sulfobacillus thermotolerans DSM 17362T, and Sulfobacillus benefaciens DSM 19468T were 94.8, 91.8 and 91.6 %, respectively.
34236956	5	28	theme	sequence	583:590	arg1	identities					592:601	the sequence identities	579:601	the sequence identities to Sulfobacillus acidophilus DSM 10332T, Sulfobacillus thermotolerans DSM 17362T, and Sulfobacillus benefaciens DSM 19468T	579:724	The results of analysis of the 16S rRNA gene sequences indicated that BGR 140T was phylogenetically related to different members of the genus Sulfobacillus, and the sequence identities to Sulfobacillus acidophilus DSM 10332T, Sulfobacillus thermotolerans DSM 17362T, and Sulfobacillus benefaciens DSM 19468T were 94.8, 91.8 and 91.6 %, respectively.
34236956	4	29	theme	optimum	367:373	arg1	25-55 °C					357:364	25-55 °C	357:364	25-55 °C (optimum 45 °C)	357:380	BGR 140T grew aerobically at 25-55 °C (optimum 45 °C) and at pH 1.5-5.0 (optimum pH 3.0).
34236956	4	29	theme	optimum	367:373	arg1	45 °C					375:379	optimum 45 °C	367:379	optimum 45 °C	367:379	BGR 140T grew aerobically at 25-55 °C (optimum 45 °C) and at pH 1.5-5.0 (optimum pH 3.0).
34236956	11	30	theme	Sulfobacillus	1250:1262	arg1	sp					1274:1275	the name Sulfobacillus harzensis sp	1241:1275	the name Sulfobacillus harzensis sp	1241:1275	On the basis of the results of phenotypic and genomic analyses, it is concluded that strain BGR 140T represents a novel species of the genus Sulfobacillus, for which the name Sulfobacillus harzensis sp.
34236956	5	31	theme	different	529:537	arg1	members					539:545	different members	529:545	different members of the genus Sulfobacillus	529:572	The results of analysis of the 16S rRNA gene sequences indicated that BGR 140T was phylogenetically related to different members of the genus Sulfobacillus, and the sequence identities to Sulfobacillus acidophilus DSM 10332T, Sulfobacillus thermotolerans DSM 17362T, and Sulfobacillus benefaciens DSM 19468T were 94.8, 91.8 and 91.6 %, respectively.
34236956	3	32	theme	140T	255:258	arg1	Cells					242:246	Cells	242:246	Cells of BGR 140T	242:258	Cells of BGR 140T were Gram-stain-positive, endospore-forming, motile and rod-shaped.
34236956	1	33	theme	polymetallic	91:102	arg1	mine					104:107	a polymetallic mine	89:107	a polymetallic mine	89:107	nov., an acidophilic bacterium inhabiting mine tailings from a polymetallic mine.
34236956	2	34	attach	isolated	170:177	arg2	140T					161:164	A mixotrophic and acidophilic bacterial strain BGR 140T	110:164	A mixotrophic and acidophilic bacterial strain BGR 140T	110:164	A mixotrophic and acidophilic bacterial strain BGR 140T was isolated from mine tailings in the Harz Mountains near Goslar, Germany.
34236956	2	34	attach	isolated	170:177	arg1	tailings					189:196	mine tailings	184:196	mine tailings in the Harz Mountains near Goslar, Germany	184:239	A mixotrophic and acidophilic bacterial strain BGR 140T was isolated from mine tailings in the Harz Mountains near Goslar, Germany.
34236956	5	35	theme	DSM	673:675	arg1	17362T					677:682	Sulfobacillus thermotolerans DSM 17362T	644:682	Sulfobacillus thermotolerans DSM 17362T	644:682	The results of analysis of the 16S rRNA gene sequences indicated that BGR 140T was phylogenetically related to different members of the genus Sulfobacillus, and the sequence identities to Sulfobacillus acidophilus DSM 10332T, Sulfobacillus thermotolerans DSM 17362T, and Sulfobacillus benefaciens DSM 19468T were 94.8, 91.8 and 91.6 %, respectively.
34236956	11	36	theme	BGR	1167:1169	arg1	140T					1171:1174	strain BGR 140T	1160:1174	strain BGR 140T	1160:1174	On the basis of the results of phenotypic and genomic analyses, it is concluded that strain BGR 140T represents a novel species of the genus Sulfobacillus, for which the name Sulfobacillus harzensis sp.
34236956	11	37	dep	results	1095:1101	arg1	basis					1082:1086	basis	1082:1086	basis	1082:1086	On the basis of the results of phenotypic and genomic analyses, it is concluded that strain BGR 140T represents a novel species of the genus Sulfobacillus, for which the name Sulfobacillus harzensis sp.
34236956	11	37	dep	results	1095:1101	arg1	the					1078:1080	the	1078:1080	the	1078:1080	On the basis of the results of phenotypic and genomic analyses, it is concluded that strain BGR 140T represents a novel species of the genus Sulfobacillus, for which the name Sulfobacillus harzensis sp.
34236956	10	38	theme	mol	1069:1071	arg1	content					1053:1059	The genomic DNA G+C content	1033:1059	The genomic DNA G+C content	1033:1059	The genomic DNA G+C content is 58.2 mol%.
34236956	10	38	theme	mol	1069:1071	arg1	%					1072:1072	58.2 mol%	1064:1072	58.2 mol%	1064:1072	The genomic DNA G+C content is 58.2 mol%.
34236956	11	39	theme	strain	1160:1165	arg1	140T					1171:1174	strain BGR 140T	1160:1174	strain BGR 140T	1160:1174	On the basis of the results of phenotypic and genomic analyses, it is concluded that strain BGR 140T represents a novel species of the genus Sulfobacillus, for which the name Sulfobacillus harzensis sp.
34236956	5	40	theme	analysis	433:440	arg1	results					422:428	The results	418:428	The results of analysis of the 16S rRNA gene sequences	418:471	The results of analysis of the 16S rRNA gene sequences indicated that BGR 140T was phylogenetically related to different members of the genus Sulfobacillus, and the sequence identities to Sulfobacillus acidophilus DSM 10332T, Sulfobacillus thermotolerans DSM 17362T, and Sulfobacillus benefaciens DSM 19468T were 94.8, 91.8 and 91.6 %, respectively.
34236956	3	41	theme	BGR	251:253	arg1	140T					255:258	BGR 140T	251:258	BGR 140T	251:258	Cells of BGR 140T were Gram-stain-positive, endospore-forming, motile and rod-shaped.
34236956	5	42	theme	BGR	488:490	arg1	140T					492:495	BGR 140T	488:495	BGR 140T	488:495	The results of analysis of the 16S rRNA gene sequences indicated that BGR 140T was phylogenetically related to different members of the genus Sulfobacillus, and the sequence identities to Sulfobacillus acidophilus DSM 10332T, Sulfobacillus thermotolerans DSM 17362T, and Sulfobacillus benefaciens DSM 19468T were 94.8, 91.8 and 91.6 %, respectively.
34236956	5	43	theme	DSM	632:634	arg1	10332T					636:641	Sulfobacillus acidophilus DSM 10332T	606:641	Sulfobacillus acidophilus DSM 10332T	606:641	The results of analysis of the 16S rRNA gene sequences indicated that BGR 140T was phylogenetically related to different members of the genus Sulfobacillus, and the sequence identities to Sulfobacillus acidophilus DSM 10332T, Sulfobacillus thermotolerans DSM 17362T, and Sulfobacillus benefaciens DSM 19468T were 94.8, 91.8 and 91.6 %, respectively.
34236956	13	44	theme	type	1322:1325	arg1	strain					1327:1332	Its type strain	1318:1332	Its type strain	1318:1332	Its type strain is BGR 140T (=DSM 109850T=JCM 39070T).
34236956	4	45	theme	BGR	328:330	arg1	140T					332:335	BGR 140T	328:335	BGR 140T	328:335	BGR 140T grew aerobically at 25-55 °C (optimum 45 °C) and at pH 1.5-5.0 (optimum pH 3.0).
34236956	13	46	theme	109850T=JCM	1352:1362	arg1	39070T					1364:1369	=DSM 109850T=JCM 39070T	1347:1369	=DSM 109850T=JCM 39070T	1347:1369	Its type strain is BGR 140T (=DSM 109850T=JCM 39070T).
34236956	13	46	theme	109850T=JCM	1352:1362	arg1	140T					1341:1344	BGR 140T	1337:1344	BGR 140T (=DSM 109850T=JCM 39070T)	1337:1370	Its type strain is BGR 140T (=DSM 109850T=JCM 39070T).
34236956	10	47	theme	DNA	1045:1047	arg1	content					1053:1059	The genomic DNA G+C content	1033:1059	The genomic DNA G+C content	1033:1059	The genomic DNA G+C content is 58.2 mol%.
34236956	10	47	theme	DNA	1045:1047	arg1	%					1072:1072	58.2 mol%	1064:1072	58.2 mol%	1064:1072	The genomic DNA G+C content is 58.2 mol%.
34236956	10	48	theme	genomic	1037:1043	arg1	content					1053:1059	The genomic DNA G+C content	1033:1059	The genomic DNA G+C content	1033:1059	The genomic DNA G+C content is 58.2 mol%.
34236956	10	48	theme	genomic	1037:1043	arg1	%					1072:1072	58.2 mol%	1064:1072	58.2 mol%	1064:1072	The genomic DNA G+C content is 58.2 mol%.
34236956	8	49	theme	polar	886:890	arg1	glycolipid					921:930	glycolipid	921:930	glycolipid	921:930	The major polar lipids were determined to be glycolipid, phospholipid and phosphatidylglycerol.
34236956	8	49	theme	polar	886:890	arg1	lipids					892:897	The major polar lipids	876:897	The major polar lipids	876:897	The major polar lipids were determined to be glycolipid, phospholipid and phosphatidylglycerol.
34236956	6	50	theme	meso-diaminopimelic	816:834	arg1	acid					836:839	meso-diaminopimelic acid	816:839	meso-diaminopimelic acid	816:839	Its cell wall peptidoglycan is A1γ, composed of meso-diaminopimelic acid.
34236956	9	51	theme	predominant	976:986	arg1	acid					994:997	The predominant fatty acid	972:997	The predominant fatty acid	972:997	The predominant fatty acid is 11-cycloheptanoyl-undecanoate.
34236956	9	51	theme	predominant	976:986	arg1	11-cycloheptanoyl-undecanoate					1002:1030	11-cycloheptanoyl-undecanoate	1002:1030	11-cycloheptanoyl-undecanoate	1002:1030	The predominant fatty acid is 11-cycloheptanoyl-undecanoate.
34236956	8	52	theme	major	880:884	arg1	glycolipid					921:930	glycolipid	921:930	glycolipid	921:930	The major polar lipids were determined to be glycolipid, phospholipid and phosphatidylglycerol.
34236956	8	52	theme	major	880:884	arg1	lipids					892:897	The major polar lipids	876:897	The major polar lipids	876:897	The major polar lipids were determined to be glycolipid, phospholipid and phosphatidylglycerol.
34236956	1	53	dep	bacterium	49:57	arg1	nov.					28:31	nov.	28:31	nov.	28:31	nov., an acidophilic bacterium inhabiting mine tailings from a polymetallic mine.
34236956	9	54	theme	fatty	988:992	arg1	acid					994:997	The predominant fatty acid	972:997	The predominant fatty acid	972:997	The predominant fatty acid is 11-cycloheptanoyl-undecanoate.
34236956	9	54	theme	fatty	988:992	arg1	11-cycloheptanoyl-undecanoate					1002:1030	11-cycloheptanoyl-undecanoate	1002:1030	11-cycloheptanoyl-undecanoate	1002:1030	The predominant fatty acid is 11-cycloheptanoyl-undecanoate.
34236956	5	55	theme	Sulfobacillus	644:656	arg1	17362T					677:682	Sulfobacillus thermotolerans DSM 17362T	644:682	Sulfobacillus thermotolerans DSM 17362T	644:682	The results of analysis of the 16S rRNA gene sequences indicated that BGR 140T was phylogenetically related to different members of the genus Sulfobacillus, and the sequence identities to Sulfobacillus acidophilus DSM 10332T, Sulfobacillus thermotolerans DSM 17362T, and Sulfobacillus benefaciens DSM 19468T were 94.8, 91.8 and 91.6 %, respectively.
34236956	4	56	theme	optimum	401:407	arg1	pH					389:390	pH 1.5-5.0	389:398	pH 1.5-5.0 (optimum pH 3.0)	389:415	BGR 140T grew aerobically at 25-55 °C (optimum 45 °C) and at pH 1.5-5.0 (optimum pH 3.0).
34236956	4	56	theme	optimum	401:407	arg1	pH					409:410	optimum pH 3.0	401:414	optimum pH 3.0	401:414	BGR 140T grew aerobically at 25-55 °C (optimum 45 °C) and at pH 1.5-5.0 (optimum pH 3.0).
34236956	11	57	theme	phenotypic	1106:1115	arg1	analyses					1129:1136	phenotypic and genomic analyses	1106:1136	phenotypic and genomic analyses	1106:1136	On the basis of the results of phenotypic and genomic analyses, it is concluded that strain BGR 140T represents a novel species of the genus Sulfobacillus, for which the name Sulfobacillus harzensis sp.
34236956	5	58	theme	gene	458:461	arg1	sequences					463:471	the 16S rRNA gene sequences	445:471	the 16S rRNA gene sequences	445:471	The results of analysis of the 16S rRNA gene sequences indicated that BGR 140T was phylogenetically related to different members of the genus Sulfobacillus, and the sequence identities to Sulfobacillus acidophilus DSM 10332T, Sulfobacillus thermotolerans DSM 17362T, and Sulfobacillus benefaciens DSM 19468T were 94.8, 91.8 and 91.6 %, respectively.
34236956	7	59	theme	respiratory	846:856	arg1	quinone					858:864	The respiratory quinone	842:864	The respiratory quinone	842:864	The respiratory quinone is DMK-6.
34236956	7	59	theme	respiratory	846:856	arg1	DMK-6					869:873	DMK-6	869:873	DMK-6	869:873	The respiratory quinone is DMK-6.
34236956	2	60	theme	mine	184:187	arg1	tailings					189:196	mine tailings	184:196	mine tailings in the Harz Mountains near Goslar, Germany	184:239	A mixotrophic and acidophilic bacterial strain BGR 140T was isolated from mine tailings in the Harz Mountains near Goslar, Germany.
34236956	5	61	theme	sequences	463:471	arg1	analysis					433:440	analysis	433:440	analysis of the 16S rRNA gene sequences	433:471	The results of analysis of the 16S rRNA gene sequences indicated that BGR 140T was phylogenetically related to different members of the genus Sulfobacillus, and the sequence identities to Sulfobacillus acidophilus DSM 10332T, Sulfobacillus thermotolerans DSM 17362T, and Sulfobacillus benefaciens DSM 19468T were 94.8, 91.8 and 91.6 %, respectively.
34236956	13	62	theme	BGR	1337:1339	arg1	39070T					1364:1369	=DSM 109850T=JCM 39070T	1347:1369	=DSM 109850T=JCM 39070T	1347:1369	Its type strain is BGR 140T (=DSM 109850T=JCM 39070T).
34236956	13	62	theme	BGR	1337:1339	arg1	140T					1341:1344	BGR 140T	1337:1344	BGR 140T (=DSM 109850T=JCM 39070T)	1337:1370	Its type strain is BGR 140T (=DSM 109850T=JCM 39070T).
34236956	1	63	theme	acidophilic	37:47	arg1	bacterium					49:57	an acidophilic bacterium	34:57	an acidophilic bacterium inhabiting mine tailings from a polymetallic mine	34:107	nov., an acidophilic bacterium inhabiting mine tailings from a polymetallic mine.
34236956	5	64	dep	Sulfobacillus	606:618	arg1	acidophilus					620:630	acidophilus	620:630	acidophilus	620:630	The results of analysis of the 16S rRNA gene sequences indicated that BGR 140T was phylogenetically related to different members of the genus Sulfobacillus, and the sequence identities to Sulfobacillus acidophilus DSM 10332T, Sulfobacillus thermotolerans DSM 17362T, and Sulfobacillus benefaciens DSM 19468T were 94.8, 91.8 and 91.6 %, respectively.
34236956	5	65	dep	Sulfobacillus	644:656	arg1	thermotolerans					658:671	thermotolerans	658:671	thermotolerans	658:671	The results of analysis of the 16S rRNA gene sequences indicated that BGR 140T was phylogenetically related to different members of the genus Sulfobacillus, and the sequence identities to Sulfobacillus acidophilus DSM 10332T, Sulfobacillus thermotolerans DSM 17362T, and Sulfobacillus benefaciens DSM 19468T were 94.8, 91.8 and 91.6 %, respectively.
31917571	9	0	theme	most	1714:1717	arg1	kinetics					1731:1738	most biochemical kinetics	1714:1738	most biochemical kinetics	1714:1738	Overall, our study substantiates the idea that most biochemical kinetics cannot be fully explained without including the subtle action of the environment where they take place naturally, in particular accounting for important factors such as excluded-volume effects and also weak nonspecific interactions when present, confinement, and fluctuations.
31917571	8	1	theme	excluded-volume	1455:1469	arg1	effects					1471:1477	excluded-volume effects	1455:1477	excluded-volume effects	1455:1477	Molecular dynamics simulations confirm that excluded-volume effects lead to an entropic force that effectively tends to push the loop closed, thereby effectively shifting the conformational ensemble of the enzyme in favor of a more stable complex isoform.
31917571	1	2	theme	crowded	155:161	arg1	environment					127:137	an extremely complex environment	106:137	an extremely complex environment	106:137	The cell is an extremely complex environment, notably highly crowded, segmented, and confining.
31917571	1	2	theme	crowded	155:161	arg1	cell					98:101	The cell	94:101	The cell	94:101	The cell is an extremely complex environment, notably highly crowded, segmented, and confining.
31917571	0	3	theme	Pushing	85:91	arg1	Role					68:71	Role	68:71	Crowding-Induced Uncompetitive Inhibition of Lactate Dehydrogenase: Role of Entropic Pushing.	0:92	Crowding-Induced Uncompetitive Inhibition of Lactate Dehydrogenase: Role of Entropic Pushing.
31917571	3	4	theme	biochemical	500:510	arg1	reactions					512:520	biochemical reactions	500:520	biochemical reactions	500:520	Effects such as excluded volume, obstructed diffusion, weak nonspecific interactions, and fluctuations all team up to steer biochemical reactions often very far from what is observed in ideal conditions.
31917571	8	5	theme	complex	1650:1656	arg1	isoform					1658:1664	a more stable complex isoform	1636:1664	a more stable complex isoform	1636:1664	Molecular dynamics simulations confirm that excluded-volume effects lead to an entropic force that effectively tends to push the loop closed, thereby effectively shifting the conformational ensemble of the enzyme in favor of a more stable complex isoform.
31917571	8	6	theme	conformational	1586:1599	arg1	ensemble					1601:1608	the conformational ensemble	1582:1608	the conformational ensemble of the enzyme	1582:1622	Molecular dynamics simulations confirm that excluded-volume effects lead to an entropic force that effectively tends to push the loop closed, thereby effectively shifting the conformational ensemble of the enzyme in favor of a more stable complex isoform.
31917571	4	7	theme	such	726:729	arg1	environments					731:742	such environments	726:742	such environments	726:742	In this paper, we use Ficoll PM70 and PEG 6000 to build an artificial crowded milieu of controlled composition and density in order to assess how such environments influence the biocatalytic activity of lactate dehydrogenase (LDH).
31917571	8	8	theme	stable	1643:1648	arg1	isoform					1658:1664	a more stable complex isoform	1636:1664	a more stable complex isoform	1636:1664	Molecular dynamics simulations confirm that excluded-volume effects lead to an entropic force that effectively tends to push the loop closed, thereby effectively shifting the conformational ensemble of the enzyme in favor of a more stable complex isoform.
31917571	8	9	theme	entropic	1490:1497	arg1	force					1499:1503	an entropic force	1487:1503	an entropic force that effectively tends to push the loop closed, thereby effectively shifting the conformational ensemble of the enzyme in favor of a more stable complex isoform	1487:1664	Molecular dynamics simulations confirm that excluded-volume effects lead to an entropic force that effectively tends to push the loop closed, thereby effectively shifting the conformational ensemble of the enzyme in favor of a more stable complex isoform.
31917571	6	10	theme	active	1207:1212	arg1	site					1214:1217	the active site	1203:1217	the active site	1203:1217	In line with previous studies on other enzymes of the same family, and in agreement with the known role of a surface loop involved in enzyme isomerization and regulation of access to the active site, we suggest that the crowding matrix interferes with the conformational ensemble of the enzyme.
31917571	7	11	theme	thwarted	1385:1392	arg1	release					1402:1408	thwarted product release	1385:1408	thwarted product release	1385:1408	This likely results in both impaired enzyme-complex isomerization and thwarted product release.
31917571	9	12	theme	biochemical	1719:1729	arg1	kinetics					1731:1738	most biochemical kinetics	1714:1738	most biochemical kinetics	1714:1738	Overall, our study substantiates the idea that most biochemical kinetics cannot be fully explained without including the subtle action of the environment where they take place naturally, in particular accounting for important factors such as excluded-volume effects and also weak nonspecific interactions when present, confinement, and fluctuations.
31917571	9	13	theme	important	1883:1891	arg1	fluctuations					2003:2014	fluctuations	2003:2014	fluctuations	2003:2014	Overall, our study substantiates the idea that most biochemical kinetics cannot be fully explained without including the subtle action of the environment where they take place naturally, in particular accounting for important factors such as excluded-volume effects and also weak nonspecific interactions when present, confinement, and fluctuations.
31917571	9	13	theme	important	1883:1891	arg1	interactions					1959:1970	weak nonspecific interactions	1942:1970	excluded-volume effects and also weak nonspecific interactions when present	1909:1983	Overall, our study substantiates the idea that most biochemical kinetics cannot be fully explained without including the subtle action of the environment where they take place naturally, in particular accounting for important factors such as excluded-volume effects and also weak nonspecific interactions when present, confinement, and fluctuations.
31917571	9	13	theme	important	1883:1891	arg1	effects					1925:1931	excluded-volume effects	1909:1931	excluded-volume effects and also weak nonspecific interactions when present	1909:1983	Overall, our study substantiates the idea that most biochemical kinetics cannot be fully explained without including the subtle action of the environment where they take place naturally, in particular accounting for important factors such as excluded-volume effects and also weak nonspecific interactions when present, confinement, and fluctuations.
31917571	9	13	theme	important	1883:1891	arg1	confinement					1986:1996	confinement	1986:1996	confinement	1986:1996	Overall, our study substantiates the idea that most biochemical kinetics cannot be fully explained without including the subtle action of the environment where they take place naturally, in particular accounting for important factors such as excluded-volume effects and also weak nonspecific interactions when present, confinement, and fluctuations.
31917571	9	13	theme	important	1883:1891	arg1	factors					1893:1899	important factors	1883:1899	important factors such as excluded-volume effects and also weak nonspecific interactions when present, confinement, and fluctuations	1883:2014	Overall, our study substantiates the idea that most biochemical kinetics cannot be fully explained without including the subtle action of the environment where they take place naturally, in particular accounting for important factors such as excluded-volume effects and also weak nonspecific interactions when present, confinement, and fluctuations.
31917571	4	14	theme	composition	679:689	arg1	milieu					658:663	an artificial crowded milieu	636:663	an artificial crowded milieu of controlled composition and density	636:701	In this paper, we use Ficoll PM70 and PEG 6000 to build an artificial crowded milieu of controlled composition and density in order to assess how such environments influence the biocatalytic activity of lactate dehydrogenase (LDH).
31917571	6	15	from	studies	1042:1048	arg1	enzymes					1059:1065	other enzymes	1053:1065	other enzymes of the same family	1053:1084	In line with previous studies on other enzymes of the same family, and in agreement with the known role of a surface loop involved in enzyme isomerization and regulation of access to the active site, we suggest that the crowding matrix interferes with the conformational ensemble of the enzyme.
31917571	2	16	theme	biochemical	270:280	arg1	reactions					282:290	biochemical reactions	270:290	biochemical reactions	270:290	Overall, there is overwhelming and ever-growing evidence that to understand how biochemical reactions proceed in vivo, one cannot separate the biochemical actors from their environment.
31917571	1	17	theme	segmented	164:172	arg1	environment					127:137	an extremely complex environment	106:137	an extremely complex environment	106:137	The cell is an extremely complex environment, notably highly crowded, segmented, and confining.
31917571	1	17	theme	segmented	164:172	arg1	cell					98:101	The cell	94:101	The cell	94:101	The cell is an extremely complex environment, notably highly crowded, segmented, and confining.
31917571	6	18	theme	conformational	1276:1289	arg1	ensemble					1291:1298	the conformational ensemble	1272:1298	the conformational ensemble of the enzyme	1272:1312	In line with previous studies on other enzymes of the same family, and in agreement with the known role of a surface loop involved in enzyme isomerization and regulation of access to the active site, we suggest that the crowding matrix interferes with the conformational ensemble of the enzyme.
31917571	7	19	theme	product	1394:1400	arg1	release					1402:1408	thwarted product release	1385:1408	thwarted product release	1385:1408	This likely results in both impaired enzyme-complex isomerization and thwarted product release.
31917571	9	20	theme	subtle	1788:1793	arg1	action					1795:1800	the subtle action	1784:1800	the subtle action of the environment where they take place naturally, in particular accounting for important factors such as excluded-volume effects and also weak nonspecific interactions when present, confinement, and fluctuations	1784:2014	Overall, our study substantiates the idea that most biochemical kinetics cannot be fully explained without including the subtle action of the environment where they take place naturally, in particular accounting for important factors such as excluded-volume effects and also weak nonspecific interactions when present, confinement, and fluctuations.
31917571	5	21	theme	uncompetitive	949:961	arg1	inhibition					963:972	uncompetitive inhibition	949:972	uncompetitive inhibition	949:972	Our measurements show that the normalized apparent affinity and maximum velocity decrease in the same fashion, a behavior reminiscent of uncompetitive inhibition, with PEG resulting in the largest reduction.
31917571	3	22	located	observed	550:557	arg1	conditions					568:577	ideal conditions	562:577	ideal conditions	562:577	Effects such as excluded volume, obstructed diffusion, weak nonspecific interactions, and fluctuations all team up to steer biochemical reactions often very far from what is observed in ideal conditions.
31917571	3	22	located	observed	550:557	arg2	what					542:545	what	542:545	what	542:545	Effects such as excluded volume, obstructed diffusion, weak nonspecific interactions, and fluctuations all team up to steer biochemical reactions often very far from what is observed in ideal conditions.
31917571	3	23	theme	nonspecific	436:446	arg1	interactions					448:459	weak nonspecific interactions	431:459	weak nonspecific interactions	431:459	Effects such as excluded volume, obstructed diffusion, weak nonspecific interactions, and fluctuations all team up to steer biochemical reactions often very far from what is observed in ideal conditions.
31917571	0	24	theme	Uncompetitive	17:29	arg1	Inhibition					31:40	Crowding-Induced Uncompetitive Inhibition	0:40	Crowding-Induced Uncompetitive Inhibition of Lactate Dehydrogenase: Role of Entropic Pushing.	0:92	Crowding-Induced Uncompetitive Inhibition of Lactate Dehydrogenase: Role of Entropic Pushing.
31917571	6	25	theme	enzyme	1307:1312	arg1	ensemble					1291:1298	the conformational ensemble	1272:1298	the conformational ensemble of the enzyme	1272:1312	In line with previous studies on other enzymes of the same family, and in agreement with the known role of a surface loop involved in enzyme isomerization and regulation of access to the active site, we suggest that the crowding matrix interferes with the conformational ensemble of the enzyme.
31917571	6	26	theme	access	1193:1198	arg1	enzyme					1154:1159	enzyme isomerization and regulation	1154:1188	enzyme	1154:1159	In line with previous studies on other enzymes of the same family, and in agreement with the known role of a surface loop involved in enzyme isomerization and regulation of access to the active site, we suggest that the crowding matrix interferes with the conformational ensemble of the enzyme.
31917571	6	26	theme	access	1193:1198	arg1	regulation					1179:1188	regulation	1179:1188	regulation	1179:1188	In line with previous studies on other enzymes of the same family, and in agreement with the known role of a surface loop involved in enzyme isomerization and regulation of access to the active site, we suggest that the crowding matrix interferes with the conformational ensemble of the enzyme.
31917571	5	27	theme	same	909:912	arg1	reminiscent					934:944	a behavior reminiscent	923:944	a behavior reminiscent of uncompetitive inhibition	923:972	Our measurements show that the normalized apparent affinity and maximum velocity decrease in the same fashion, a behavior reminiscent of uncompetitive inhibition, with PEG resulting in the largest reduction.
31917571	5	27	theme	same	909:912	arg1	fashion					914:920	the same fashion	905:920	the same fashion	905:920	Our measurements show that the normalized apparent affinity and maximum velocity decrease in the same fashion, a behavior reminiscent of uncompetitive inhibition, with PEG resulting in the largest reduction.
31917571	6	28	theme	other	1053:1057	arg1	enzymes					1059:1065	other enzymes	1053:1065	other enzymes of the same family	1053:1084	In line with previous studies on other enzymes of the same family, and in agreement with the known role of a surface loop involved in enzyme isomerization and regulation of access to the active site, we suggest that the crowding matrix interferes with the conformational ensemble of the enzyme.
31917571	6	29	theme	previous	1033:1040	arg1	studies					1042:1048	previous studies	1033:1048	previous studies on other enzymes of the same family	1033:1084	In line with previous studies on other enzymes of the same family, and in agreement with the known role of a surface loop involved in enzyme isomerization and regulation of access to the active site, we suggest that the crowding matrix interferes with the conformational ensemble of the enzyme.
31917571	0	30	theme	Crowding-Induced	0:15	arg1	Inhibition					31:40	Crowding-Induced Uncompetitive Inhibition	0:40	Crowding-Induced Uncompetitive Inhibition of Lactate Dehydrogenase: Role of Entropic Pushing.	0:92	Crowding-Induced Uncompetitive Inhibition of Lactate Dehydrogenase: Role of Entropic Pushing.
31917571	2	31	from	environment	363:373	arg1	separate					320:327	separate	320:327	separate	320:327	Overall, there is overwhelming and ever-growing evidence that to understand how biochemical reactions proceed in vivo, one cannot separate the biochemical actors from their environment.
31917571	3	32	theme	ideal	562:566	arg1	conditions					568:577	ideal conditions	562:577	ideal conditions	562:577	Effects such as excluded volume, obstructed diffusion, weak nonspecific interactions, and fluctuations all team up to steer biochemical reactions often very far from what is observed in ideal conditions.
31917571	1	33	theme	confining	179:187	arg1	environment					127:137	an extremely complex environment	106:137	an extremely complex environment	106:137	The cell is an extremely complex environment, notably highly crowded, segmented, and confining.
31917571	1	33	theme	confining	179:187	arg1	cell					98:101	The cell	94:101	The cell	94:101	The cell is an extremely complex environment, notably highly crowded, segmented, and confining.
31917571	4	34	theme	dehydrogenase	791:803	arg1	activity					771:778	the biocatalytic activity	754:778	the biocatalytic activity of lactate dehydrogenase (LDH)	754:809	In this paper, we use Ficoll PM70 and PEG 6000 to build an artificial crowded milieu of controlled composition and density in order to assess how such environments influence the biocatalytic activity of lactate dehydrogenase (LDH).
31917571	5	35	theme	behavior	925:932	arg1	reminiscent					934:944	a behavior reminiscent	923:944	a behavior reminiscent of uncompetitive inhibition	923:972	Our measurements show that the normalized apparent affinity and maximum velocity decrease in the same fashion, a behavior reminiscent of uncompetitive inhibition, with PEG resulting in the largest reduction.
31917571	5	35	theme	behavior	925:932	arg1	fashion					914:920	the same fashion	905:920	the same fashion	905:920	Our measurements show that the normalized apparent affinity and maximum velocity decrease in the same fashion, a behavior reminiscent of uncompetitive inhibition, with PEG resulting in the largest reduction.
31917571	7	36	theme	impaired	1343:1350	arg1	isomerization					1367:1379	impaired enzyme-complex isomerization	1343:1379	impaired enzyme-complex isomerization	1343:1379	This likely results in both impaired enzyme-complex isomerization and thwarted product release.
31917571	7	37	theme	enzyme-complex	1352:1365	arg1	isomerization					1367:1379	impaired enzyme-complex isomerization	1343:1379	impaired enzyme-complex isomerization	1343:1379	This likely results in both impaired enzyme-complex isomerization and thwarted product release.
31917571	5	38	theme	maximum	876:882	arg1	velocity					884:891	maximum velocity	876:891	maximum velocity	876:891	Our measurements show that the normalized apparent affinity and maximum velocity decrease in the same fashion, a behavior reminiscent of uncompetitive inhibition, with PEG resulting in the largest reduction.
31917571	4	39	theme	Ficoll	602:607	arg1	PM70					609:612	Ficoll PM70	602:612	Ficoll PM70	602:612	In this paper, we use Ficoll PM70 and PEG 6000 to build an artificial crowded milieu of controlled composition and density in order to assess how such environments influence the biocatalytic activity of lactate dehydrogenase (LDH).
31917571	0	40	theme	Dehydrogenase	53:65	arg1	Inhibition					31:40	Crowding-Induced Uncompetitive Inhibition	0:40	Crowding-Induced Uncompetitive Inhibition of Lactate Dehydrogenase: Role of Entropic Pushing.	0:92	Crowding-Induced Uncompetitive Inhibition of Lactate Dehydrogenase: Role of Entropic Pushing.
31917571	4	41	theme	controlled	668:677	arg1	composition					679:689	controlled composition	668:689	controlled composition	668:689	In this paper, we use Ficoll PM70 and PEG 6000 to build an artificial crowded milieu of controlled composition and density in order to assess how such environments influence the biocatalytic activity of lactate dehydrogenase (LDH).
31917571	2	42	theme	ever-growing	225:236	arg1	evidence					238:245	overwhelming and ever-growing evidence that to understand how biochemical reactions proceed in vivo, one cannot separate the biochemical actors from their environment	208:373	overwhelming and ever-growing evidence that to understand how biochemical reactions proceed in vivo, one cannot separate the biochemical actors from their environment	208:373	Overall, there is overwhelming and ever-growing evidence that to understand how biochemical reactions proceed in vivo, one cannot separate the biochemical actors from their environment.
31917571	5	43	theme	normalized	843:852	arg1	affinity					863:870	the normalized apparent affinity	839:870	the normalized apparent affinity	839:870	Our measurements show that the normalized apparent affinity and maximum velocity decrease in the same fashion, a behavior reminiscent of uncompetitive inhibition, with PEG resulting in the largest reduction.
31917571	4	44	theme	PEG	618:620	arg1	6000					622:625	PEG 6000	618:625	PEG 6000	618:625	In this paper, we use Ficoll PM70 and PEG 6000 to build an artificial crowded milieu of controlled composition and density in order to assess how such environments influence the biocatalytic activity of lactate dehydrogenase (LDH).
31917571	3	45	theme	weak	431:434	arg1	interactions					448:459	weak nonspecific interactions	431:459	weak nonspecific interactions	431:459	Effects such as excluded volume, obstructed diffusion, weak nonspecific interactions, and fluctuations all team up to steer biochemical reactions often very far from what is observed in ideal conditions.
31917571	6	46	with	agreement	1094:1102	arg1	role					1119:1122	the known role	1109:1122	the known role of a surface loop involved in enzyme isomerization and regulation of access to the active site	1109:1217	In line with previous studies on other enzymes of the same family, and in agreement with the known role of a surface loop involved in enzyme isomerization and regulation of access to the active site, we suggest that the crowding matrix interferes with the conformational ensemble of the enzyme.
31917571	0	47	theme	Lactate	45:51	arg1	Dehydrogenase					53:65	Lactate Dehydrogenase	45:65	Lactate Dehydrogenase	45:65	Crowding-Induced Uncompetitive Inhibition of Lactate Dehydrogenase: Role of Entropic Pushing.
31917571	8	48	theme	Molecular	1411:1419	arg1	simulations					1430:1440	Molecular dynamics simulations	1411:1440	Molecular dynamics simulations	1411:1440	Molecular dynamics simulations confirm that excluded-volume effects lead to an entropic force that effectively tends to push the loop closed, thereby effectively shifting the conformational ensemble of the enzyme in favor of a more stable complex isoform.
31917571	4	49	theme	lactate	783:789	arg1	dehydrogenase					791:803	lactate dehydrogenase	783:803	lactate dehydrogenase (LDH)	783:809	In this paper, we use Ficoll PM70 and PEG 6000 to build an artificial crowded milieu of controlled composition and density in order to assess how such environments influence the biocatalytic activity of lactate dehydrogenase (LDH).
31917571	4	49	theme	lactate	783:789	arg1	LDH					806:808	LDH	806:808	LDH	806:808	In this paper, we use Ficoll PM70 and PEG 6000 to build an artificial crowded milieu of controlled composition and density in order to assess how such environments influence the biocatalytic activity of lactate dehydrogenase (LDH).
31917571	3	50	theme	excluded	392:399	arg1	volume					401:406	excluded volume	392:406	excluded volume	392:406	Effects such as excluded volume, obstructed diffusion, weak nonspecific interactions, and fluctuations all team up to steer biochemical reactions often very far from what is observed in ideal conditions.
31917571	5	51	theme	apparent	854:861	arg1	affinity					863:870	the normalized apparent affinity	839:870	the normalized apparent affinity	839:870	Our measurements show that the normalized apparent affinity and maximum velocity decrease in the same fashion, a behavior reminiscent of uncompetitive inhibition, with PEG resulting in the largest reduction.
31917571	6	52	theme	crowding	1240:1247	arg1	matrix					1249:1254	the crowding matrix	1236:1254	the crowding matrix	1236:1254	In line with previous studies on other enzymes of the same family, and in agreement with the known role of a surface loop involved in enzyme isomerization and regulation of access to the active site, we suggest that the crowding matrix interferes with the conformational ensemble of the enzyme.
31917571	6	53	dep	enzyme	1154:1159	arg1	isomerization					1161:1173	isomerization	1161:1173	isomerization	1161:1173	In line with previous studies on other enzymes of the same family, and in agreement with the known role of a surface loop involved in enzyme isomerization and regulation of access to the active site, we suggest that the crowding matrix interferes with the conformational ensemble of the enzyme.
31917571	2	54	theme	overwhelming	208:219	arg1	evidence					238:245	overwhelming and ever-growing evidence that to understand how biochemical reactions proceed in vivo, one cannot separate the biochemical actors from their environment	208:373	overwhelming and ever-growing evidence that to understand how biochemical reactions proceed in vivo, one cannot separate the biochemical actors from their environment	208:373	Overall, there is overwhelming and ever-growing evidence that to understand how biochemical reactions proceed in vivo, one cannot separate the biochemical actors from their environment.
31917571	2	55	theme	biochemical	333:343	arg1	actors					345:350	the biochemical actors	329:350	the biochemical actors	329:350	Overall, there is overwhelming and ever-growing evidence that to understand how biochemical reactions proceed in vivo, one cannot separate the biochemical actors from their environment.
31917571	5	56	theme	largest	1001:1007	arg1	reduction					1009:1017	the largest reduction	997:1017	the largest reduction	997:1017	Our measurements show that the normalized apparent affinity and maximum velocity decrease in the same fashion, a behavior reminiscent of uncompetitive inhibition, with PEG resulting in the largest reduction.
31917571	1	57	theme	complex	119:125	arg1	environment					127:137	an extremely complex environment	106:137	an extremely complex environment	106:137	The cell is an extremely complex environment, notably highly crowded, segmented, and confining.
31917571	1	57	theme	complex	119:125	arg1	cell					98:101	The cell	94:101	The cell	94:101	The cell is an extremely complex environment, notably highly crowded, segmented, and confining.
31917571	9	58	theme	environment	1809:1819	arg1	action					1795:1800	the subtle action	1784:1800	the subtle action of the environment where they take place naturally, in particular accounting for important factors such as excluded-volume effects and also weak nonspecific interactions when present, confinement, and fluctuations	1784:2014	Overall, our study substantiates the idea that most biochemical kinetics cannot be fully explained without including the subtle action of the environment where they take place naturally, in particular accounting for important factors such as excluded-volume effects and also weak nonspecific interactions when present, confinement, and fluctuations.
31917571	8	59	theme	closed	1545:1550	arg1	loop					1540:1543	the loop	1536:1543	the loop closed	1536:1550	Molecular dynamics simulations confirm that excluded-volume effects lead to an entropic force that effectively tends to push the loop closed, thereby effectively shifting the conformational ensemble of the enzyme in favor of a more stable complex isoform.
31917571	8	60	theme	dynamics	1421:1428	arg1	simulations					1430:1440	Molecular dynamics simulations	1411:1440	Molecular dynamics simulations	1411:1440	Molecular dynamics simulations confirm that excluded-volume effects lead to an entropic force that effectively tends to push the loop closed, thereby effectively shifting the conformational ensemble of the enzyme in favor of a more stable complex isoform.
31917571	5	61	theme	inhibition	963:972	arg1	reminiscent					934:944	a behavior reminiscent	923:944	a behavior reminiscent of uncompetitive inhibition	923:972	Our measurements show that the normalized apparent affinity and maximum velocity decrease in the same fashion, a behavior reminiscent of uncompetitive inhibition, with PEG resulting in the largest reduction.
31917571	5	61	theme	inhibition	963:972	arg1	fashion					914:920	the same fashion	905:920	the same fashion	905:920	Our measurements show that the normalized apparent affinity and maximum velocity decrease in the same fashion, a behavior reminiscent of uncompetitive inhibition, with PEG resulting in the largest reduction.
31917571	4	62	theme	biocatalytic	758:769	arg1	activity					771:778	the biocatalytic activity	754:778	the biocatalytic activity of lactate dehydrogenase (LDH)	754:809	In this paper, we use Ficoll PM70 and PEG 6000 to build an artificial crowded milieu of controlled composition and density in order to assess how such environments influence the biocatalytic activity of lactate dehydrogenase (LDH).
31917571	9	63	theme	weak	1942:1945	arg1	interactions					1959:1970	weak nonspecific interactions	1942:1970	excluded-volume effects and also weak nonspecific interactions when present	1909:1983	Overall, our study substantiates the idea that most biochemical kinetics cannot be fully explained without including the subtle action of the environment where they take place naturally, in particular accounting for important factors such as excluded-volume effects and also weak nonspecific interactions when present, confinement, and fluctuations.
31917571	4	64	theme	density	695:701	arg1	milieu					658:663	an artificial crowded milieu	636:663	an artificial crowded milieu of controlled composition and density	636:701	In this paper, we use Ficoll PM70 and PEG 6000 to build an artificial crowded milieu of controlled composition and density in order to assess how such environments influence the biocatalytic activity of lactate dehydrogenase (LDH).
31917571	8	65	theme	enzyme	1617:1622	arg1	ensemble					1601:1608	the conformational ensemble	1582:1608	the conformational ensemble of the enzyme	1582:1622	Molecular dynamics simulations confirm that excluded-volume effects lead to an entropic force that effectively tends to push the loop closed, thereby effectively shifting the conformational ensemble of the enzyme in favor of a more stable complex isoform.
31917571	6	66	theme	known	1113:1117	arg1	role					1119:1122	the known role	1109:1122	the known role of a surface loop involved in enzyme isomerization and regulation of access to the active site	1109:1217	In line with previous studies on other enzymes of the same family, and in agreement with the known role of a surface loop involved in enzyme isomerization and regulation of access to the active site, we suggest that the crowding matrix interferes with the conformational ensemble of the enzyme.
31917571	6	67	with	line	1023:1026	arg1	studies					1042:1048	previous studies	1033:1048	previous studies on other enzymes of the same family	1033:1084	In line with previous studies on other enzymes of the same family, and in agreement with the known role of a surface loop involved in enzyme isomerization and regulation of access to the active site, we suggest that the crowding matrix interferes with the conformational ensemble of the enzyme.
31917571	0	68	dep	Inhibition	31:40	arg1	Role					68:71	Role	68:71	Crowding-Induced Uncompetitive Inhibition of Lactate Dehydrogenase: Role of Entropic Pushing.	0:92	Crowding-Induced Uncompetitive Inhibition of Lactate Dehydrogenase: Role of Entropic Pushing.
31917571	9	69	theme	nonspecific	1947:1957	arg1	interactions					1959:1970	weak nonspecific interactions	1942:1970	excluded-volume effects and also weak nonspecific interactions when present	1909:1983	Overall, our study substantiates the idea that most biochemical kinetics cannot be fully explained without including the subtle action of the environment where they take place naturally, in particular accounting for important factors such as excluded-volume effects and also weak nonspecific interactions when present, confinement, and fluctuations.
31917571	6	70	theme	same	1074:1077	arg1	family					1079:1084	the same family	1070:1084	the same family	1070:1084	In line with previous studies on other enzymes of the same family, and in agreement with the known role of a surface loop involved in enzyme isomerization and regulation of access to the active site, we suggest that the crowding matrix interferes with the conformational ensemble of the enzyme.
31917571	6	71	theme	family	1079:1084	arg1	enzymes					1059:1065	other enzymes	1053:1065	other enzymes of the same family	1053:1084	In line with previous studies on other enzymes of the same family, and in agreement with the known role of a surface loop involved in enzyme isomerization and regulation of access to the active site, we suggest that the crowding matrix interferes with the conformational ensemble of the enzyme.
31917571	6	72	theme	loop	1137:1140	arg1	role					1119:1122	the known role	1109:1122	the known role of a surface loop involved in enzyme isomerization and regulation of access to the active site	1109:1217	In line with previous studies on other enzymes of the same family, and in agreement with the known role of a surface loop involved in enzyme isomerization and regulation of access to the active site, we suggest that the crowding matrix interferes with the conformational ensemble of the enzyme.
31917571	4	73	theme	crowded	650:656	arg1	milieu					658:663	an artificial crowded milieu	636:663	an artificial crowded milieu of controlled composition and density	636:701	In this paper, we use Ficoll PM70 and PEG 6000 to build an artificial crowded milieu of controlled composition and density in order to assess how such environments influence the biocatalytic activity of lactate dehydrogenase (LDH).
31917571	3	74	theme	obstructed	409:418	arg1	diffusion					420:428	obstructed diffusion	409:428	obstructed diffusion	409:428	Effects such as excluded volume, obstructed diffusion, weak nonspecific interactions, and fluctuations all team up to steer biochemical reactions often very far from what is observed in ideal conditions.
31917571	6	75	theme	surface	1129:1135	arg1	loop					1137:1140	a surface loop	1127:1140	a surface loop involved in enzyme isomerization and regulation of access to the active site	1127:1217	In line with previous studies on other enzymes of the same family, and in agreement with the known role of a surface loop involved in enzyme isomerization and regulation of access to the active site, we suggest that the crowding matrix interferes with the conformational ensemble of the enzyme.
31917571	4	76	theme	artificial	639:648	arg1	milieu					658:663	an artificial crowded milieu	636:663	an artificial crowded milieu of controlled composition and density	636:701	In this paper, we use Ficoll PM70 and PEG 6000 to build an artificial crowded milieu of controlled composition and density in order to assess how such environments influence the biocatalytic activity of lactate dehydrogenase (LDH).
31917571	9	77	theme	excluded-volume	1909:1923	arg1	effects					1925:1931	excluded-volume effects	1909:1931	excluded-volume effects and also weak nonspecific interactions when present	1909:1983	Overall, our study substantiates the idea that most biochemical kinetics cannot be fully explained without including the subtle action of the environment where they take place naturally, in particular accounting for important factors such as excluded-volume effects and also weak nonspecific interactions when present, confinement, and fluctuations.
34176630	10	0	from	difference	1381:1390	arg1	contents					1431:1438	medial and ventral contents	1412:1438	medial and ventral contents	1412:1438	Similarly, the difference between rumen pH in medial and ventral contents was reduced at ALT1 with high alternation level but was not affected at ALT4.
34176630	7	1	theme	relative	1059:1066	arg1	h					1057:1057	-0.5, 1, 2.5, 4, 5.5, and 7 h	1029:1057	-0.5, 1, 2.5, 4, 5.5, and 7 h relative to morning feeding (0800 h)	1029:1094	At d 7 (baseline), 8 (ALT1), and 14 (ALT4) of each period, rumen fluid, blood, urine, and quarter milk (i.e., back right quarter) samples were collected at -0.5, 1, 2.5, 4, 5.5, and 7 h relative to morning feeding (0800 h).
34176630	14	2	theme	blood	2134:2138	arg1	pH					2140:2141	blood pH	2134:2141	blood pH	2134:2141	No differences in blood pH were detected among starch alternation levels.
34176630	8	3	theme	milk	1164:1167	arg1	composition					1178:1188	milk chemical composition	1164:1188	milk chemical composition	1164:1188	No differences were observed in dry matter intake, milk yield, and milk chemical composition.
34176630	13	4	theme	alternation	1994:2004	arg1	level					2006:2010	the high alternation level	1985:2010	the high alternation level	1985:2010	In both rumen medial and ventral fluids, the high alternation level showed higher propionate and lower acetate proportions compared with low and medium alternation levels.
34176630	3	5	theme	14-d	439:442	arg1	period					444:449	Each 14-d period	434:449	Each 14-d period	434:449	Each 14-d period consisted of a 7-d baseline period and 7-d alternating period where diets alternated day to day.
34176630	15	6	theme	high	2259:2262	arg1	level					2276:2280	the high alternation level	2255:2280	the high alternation level	2255:2280	However, glucose concentrations tended to be higher in cows from the high alternation level.
34176630	9	7	theme	medial	1197:1202	arg1	pH					1204:1205	Rumen medial pH	1191:1205	Rumen medial pH	1191:1205	Rumen medial pH was lower in the high alternation level compared with the low or medium alternation levels at ALT1 but did not differ among starch alternation levels at ALT4.
34176630	17	8	theme	alternation	2484:2494	arg1	levels					2496:2501	alternation levels	2484:2501	alternation levels (i.e., low, medium, and high)	2484:2531	In urine, no differences in pH or l-lactate concentrations were detected among alternation levels (i.e., low, medium, and high).
34176630	4	9	theme	dry	632:634	arg1	basis					643:647	dry matter basis	632:647	dry matter basis	632:647	During the baseline period, all cows were fed a control diet containing 21% starch (dry matter basis).
34176630	4	9	theme	dry	632:634	arg1	starch					624:629	21% starch	620:629	21% starch (dry matter basis)	620:648	During the baseline period, all cows were fed a control diet containing 21% starch (dry matter basis).
34176630	3	10	theme	baseline	470:477	arg1	period					479:484	a 7-d baseline period	464:484	a 7-d baseline period	464:484	Each 14-d period consisted of a 7-d baseline period and 7-d alternating period where diets alternated day to day.
34176630	11	11	theme	level	1627:1631	arg1	fluid					1597:1601	the rumen medial fluid	1580:1601	the rumen medial fluid of the high alternation level at 7 h relative to morning feeding	1580:1666	Total volatile fatty acid (VFA) concentrations were higher in the rumen medial fluid of the high alternation level at 7 h relative to morning feeding compared with those from the low and medium alternation levels.
34176630	4	12	contain	containing	609:618	arg2	starch					624:629	21% starch	620:629	21% starch (dry matter basis)	620:648	During the baseline period, all cows were fed a control diet containing 21% starch (dry matter basis).
34176630	4	12	contain	containing	609:618	arg1	diet					604:607	a control diet	594:607	a control diet containing 21% starch (dry matter basis)	594:648	During the baseline period, all cows were fed a control diet containing 21% starch (dry matter basis).
34176630	4	12	contain	containing	609:618	arg2	basis					643:647	dry matter basis	632:647	dry matter basis	632:647	During the baseline period, all cows were fed a control diet containing 21% starch (dry matter basis).
34176630	16	13	theme	alternation	2386:2396	arg1	level					2398:2402	the starch alternation level	2375:2402	the starch alternation level	2375:2402	l-Lactate concentrations in blood were higher in ALT1 than in ALT4 but were not affected by the starch alternation level.
34176630	1	14	theme	alternations	142:153	arg1	effect					115:120	the effect	111:120	the effect of controlled daily alternations in dietary starch level on changes in rumen environment, blood, urine, and milk metabolites of dairy cows	111:259	The aim of this study was to investigate the effect of controlled daily alternations in dietary starch level on changes in rumen environment, blood, urine, and milk metabolites of dairy cows.
34176630	2	15	with	design	351:356	arg1	periods					368:374	14-d periods	363:374	14-d periods	363:374	Six multiparous mid-lactation Holstein cows were used in a replicated 3 × 3 Latin square design with 14-d periods and 3 alternating levels of dietary starch as treatments.
34176630	11	16	theme	high	1610:1613	arg1	level					1627:1631	the high alternation level	1606:1631	the high alternation level at 7 h relative to morning feeding	1606:1666	Total volatile fatty acid (VFA) concentrations were higher in the rumen medial fluid of the high alternation level at 7 h relative to morning feeding compared with those from the low and medium alternation levels.
34176630	3	17	theme	alternating	494:504	arg1	period					506:511	7-d alternating period	490:511	7-d alternating period	490:511	Each 14-d period consisted of a 7-d baseline period and 7-d alternating period where diets alternated day to day.
34176630	1	18	theme	dietary	158:164	arg1	level					173:177	dietary starch level	158:177	dietary starch level	158:177	The aim of this study was to investigate the effect of controlled daily alternations in dietary starch level on changes in rumen environment, blood, urine, and milk metabolites of dairy cows.
34176630	9	19	theme	high	1224:1227	arg1	level					1241:1245	the high alternation level	1220:1245	the high alternation level	1220:1245	Rumen medial pH was lower in the high alternation level compared with the low or medium alternation levels at ALT1 but did not differ among starch alternation levels at ALT4.
34176630	12	20	theme	alternation	1924:1934	arg1	levels					1936:1941	starch alternation levels	1917:1941	starch alternation levels	1917:1941	Similarly, total VFA concentrations constantly increased and were the highest in the ventral rumen fluid at 7 h relative to morning feeding, although no differences were detected among starch alternation levels.
34176630	10	21	theme	high	1465:1468	arg1	level					1482:1486	high alternation level	1465:1486	high alternation level	1465:1486	Similarly, the difference between rumen pH in medial and ventral contents was reduced at ALT1 with high alternation level but was not affected at ALT4.
34176630	13	22	theme	rumen	1952:1956	arg1	fluids					1977:1982	both rumen medial and ventral fluids	1947:1982	both rumen medial and ventral fluids	1947:1982	In both rumen medial and ventral fluids, the high alternation level showed higher propionate and lower acetate proportions compared with low and medium alternation levels.
34176630	19	23	dep	42	2705:2706	arg1	to					2702:2703	to	2702:2703	to	2702:2703	According to these results, it seems that the daily dietary starch alternation from 21% up to 42% (dry matter basis) is able to affect the ruminal fluid, especially during the first alternation.
34176630	11	24	theme	fatty	1533:1537	arg1	VFA					1545:1547	VFA	1545:1547	VFA	1545:1547	Total volatile fatty acid (VFA) concentrations were higher in the rumen medial fluid of the high alternation level at 7 h relative to morning feeding compared with those from the low and medium alternation levels.
34176630	11	24	theme	fatty	1533:1537	arg1	acid					1539:1542	fatty acid	1533:1542	Total volatile fatty acid (VFA) concentrations	1518:1563	Total volatile fatty acid (VFA) concentrations were higher in the rumen medial fluid of the high alternation level at 7 h relative to morning feeding compared with those from the low and medium alternation levels.
34176630	18	25	theme	milk	2563:2566	arg1	pH					2568:2569	milk pH	2563:2569	milk pH	2563:2569	Similarly, no differences in milk pH were detected among alternation levels.
34176630	6	26	theme	experimental	777:788	arg1	diets					790:794	The 3 experimental diets	771:794	The 3 experimental diets	771:794	The 3 experimental diets contained 28% (low), 35% (medium), and 42% (high) starch (dry matter basis).
34176630	9	27	dep	alternation	1279:1289	arg1	medium					1272:1277	medium	1272:1277	medium	1272:1277	Rumen medial pH was lower in the high alternation level compared with the low or medium alternation levels at ALT1 but did not differ among starch alternation levels at ALT4.
34176630	12	28	theme	ventral	1817:1823	arg1	fluid					1831:1835	the ventral rumen fluid	1813:1835	the ventral rumen fluid	1813:1835	Similarly, total VFA concentrations constantly increased and were the highest in the ventral rumen fluid at 7 h relative to morning feeding, although no differences were detected among starch alternation levels.
34176630	4	29	theme	21	620:621	arg1	%					622:622	%	622:622	%	622:622	During the baseline period, all cows were fed a control diet containing 21% starch (dry matter basis).
34176630	11	30	theme	medial	1590:1595	arg1	fluid					1597:1601	the rumen medial fluid	1580:1601	the rumen medial fluid of the high alternation level at 7 h relative to morning feeding	1580:1666	Total volatile fatty acid (VFA) concentrations were higher in the rumen medial fluid of the high alternation level at 7 h relative to morning feeding compared with those from the low and medium alternation levels.
34176630	9	31	theme	low	1265:1267	arg1	levels					1291:1296	the low or medium alternation levels	1261:1296	the low or medium alternation levels	1261:1296	Rumen medial pH was lower in the high alternation level compared with the low or medium alternation levels at ALT1 but did not differ among starch alternation levels at ALT4.
34176630	5	32	from	diets	741:745	arg1	d					750:750	d 8, 10, 12, and 14	750:768	d 8, 10, 12, and 14	750:768	During the alternating period, the control diet was replaced with 1 of the 3 experimental diets on d 8, 10, 12, and 14.
34176630	0	33	theme	Holstein	49:56	arg1	cows					64:67	Holstein dairy cows	49:67	Holstein dairy cows	49:67	Daily alternation of the dietary starch level in Holstein dairy cows.
34176630	9	34	theme	alternation	1338:1348	arg1	levels					1350:1355	starch alternation levels	1331:1355	starch alternation levels at ALT4	1331:1363	Rumen medial pH was lower in the high alternation level compared with the low or medium alternation levels at ALT1 but did not differ among starch alternation levels at ALT4.
34176630	6	35	dep	%	808:808	arg1	low					811:813	low	811:813	low	811:813	The 3 experimental diets contained 28% (low), 35% (medium), and 42% (high) starch (dry matter basis).
34176630	6	35	dep	%	808:808	arg1	basis					865:869	dry matter basis	854:869	dry matter basis	854:869	The 3 experimental diets contained 28% (low), 35% (medium), and 42% (high) starch (dry matter basis).
34176630	6	35	dep	%	808:808	arg1	starch					846:851	starch	846:851	starch	846:851	The 3 experimental diets contained 28% (low), 35% (medium), and 42% (high) starch (dry matter basis).
34176630	1	36	theme	controlled	125:134	arg1	alternations					142:153	controlled daily alternations	125:153	controlled daily alternations in dietary starch level	125:177	The aim of this study was to investigate the effect of controlled daily alternations in dietary starch level on changes in rumen environment, blood, urine, and milk metabolites of dairy cows.
34176630	19	37	from	%	2697:2697	arg1	alternation					2678:2688	the daily dietary starch alternation	2653:2688	the daily dietary starch alternation from 21% up to 42% (dry matter basis)	2653:2726	According to these results, it seems that the daily dietary starch alternation from 21% up to 42% (dry matter basis) is able to affect the ruminal fluid, especially during the first alternation.
34176630	19	37	from	%	2697:2697	arg1	able					2731:2734	able	2731:2734	able	2731:2734	According to these results, it seems that the daily dietary starch alternation from 21% up to 42% (dry matter basis) is able to affect the ruminal fluid, especially during the first alternation.
34176630	11	38	theme	alternation	1712:1722	arg1	levels					1724:1729	alternation levels	1712:1729	alternation levels	1712:1729	Total volatile fatty acid (VFA) concentrations were higher in the rumen medial fluid of the high alternation level at 7 h relative to morning feeding compared with those from the low and medium alternation levels.
34176630	2	39	used	used	311:314	arg2	cows					301:304	Six multiparous mid-lactation Holstein cows	262:304	Six multiparous mid-lactation Holstein cows	262:304	Six multiparous mid-lactation Holstein cows were used in a replicated 3 × 3 Latin square design with 14-d periods and 3 alternating levels of dietary starch as treatments.
34176630	2	39	used	used	311:314	arg2	treatments					422:431	treatments	422:431	treatments	422:431	Six multiparous mid-lactation Holstein cows were used in a replicated 3 × 3 Latin square design with 14-d periods and 3 alternating levels of dietary starch as treatments.
34176630	19	40	theme	matter	2714:2719	arg1	basis					2721:2725	dry matter basis	2710:2725	dry matter basis	2710:2725	According to these results, it seems that the daily dietary starch alternation from 21% up to 42% (dry matter basis) is able to affect the ruminal fluid, especially during the first alternation.
34176630	19	40	theme	matter	2714:2719	arg1	%					2697:2697	21%	2695:2697	21% up to 42% (dry matter basis)	2695:2726	According to these results, it seems that the daily dietary starch alternation from 21% up to 42% (dry matter basis) is able to affect the ruminal fluid, especially during the first alternation.
34176630	2	41	with	levels	394:399	arg1	periods					368:374	14-d periods	363:374	14-d periods	363:374	Six multiparous mid-lactation Holstein cows were used in a replicated 3 × 3 Latin square design with 14-d periods and 3 alternating levels of dietary starch as treatments.
34176630	11	42	theme	Total	1518:1522	arg1	concentrations					1550:1563	Total volatile fatty acid (VFA) concentrations	1518:1563	Total volatile fatty acid (VFA) concentrations	1518:1563	Total volatile fatty acid (VFA) concentrations were higher in the rumen medial fluid of the high alternation level at 7 h relative to morning feeding compared with those from the low and medium alternation levels.
34176630	21	43	theme	day-to-day	2966:2975	arg1	alternations					2977:2988	day-to-day alternations	2966:2988	day-to-day alternations	2966:2988	This study indicates that cows can cope with day-to-day alternations in type of rumen fermentable organic matter; however, longer-term effects on performance and health should be addressed in future studies.
34176630	13	44	theme	alternation	2096:2106	arg1	levels					2108:2113	low and medium alternation levels	2081:2113	low and medium alternation levels	2081:2113	In both rumen medial and ventral fluids, the high alternation level showed higher propionate and lower acetate proportions compared with low and medium alternation levels.
34176630	5	45	theme	alternating	662:672	arg1	period					674:679	the alternating period	658:679	the alternating period	658:679	During the alternating period, the control diet was replaced with 1 of the 3 experimental diets on d 8, 10, 12, and 14.
34176630	11	46	theme	relative	1640:1647	arg1	level					1627:1631	the high alternation level	1606:1631	the high alternation level at 7 h relative to morning feeding	1606:1666	Total volatile fatty acid (VFA) concentrations were higher in the rumen medial fluid of the high alternation level at 7 h relative to morning feeding compared with those from the low and medium alternation levels.
34176630	21	47	theme	longer-term	3044:3054	arg1	effects					3056:3062	longer-term effects	3044:3062	longer-term effects on performance and health	3044:3088	This study indicates that cows can cope with day-to-day alternations in type of rumen fermentable organic matter; however, longer-term effects on performance and health should be addressed in future studies.
34176630	6	48	dep	%	837:837	arg1	high					840:843	high	840:843	high	840:843	The 3 experimental diets contained 28% (low), 35% (medium), and 42% (high) starch (dry matter basis).
34176630	0	49	theme	Daily	0:4	arg1	alternation					6:16	Daily alternation	0:16	Daily alternation of the dietary starch level in Holstein dairy cows.	0:68	Daily alternation of the dietary starch level in Holstein dairy cows.
34176630	21	50	theme	organic	3019:3025	arg1	matter					3027:3032	rumen fermentable organic matter	3001:3032	rumen fermentable organic matter	3001:3032	This study indicates that cows can cope with day-to-day alternations in type of rumen fermentable organic matter; however, longer-term effects on performance and health should be addressed in future studies.
34176630	17	51	theme	l-lactate	2439:2447	arg1	concentrations					2449:2462	l-lactate concentrations	2439:2462	l-lactate concentrations	2439:2462	In urine, no differences in pH or l-lactate concentrations were detected among alternation levels (i.e., low, medium, and high).
34176630	7	52	theme	quarter	963:969	arg1	milk					971:974	quarter milk	963:974	quarter milk	963:974	At d 7 (baseline), 8 (ALT1), and 14 (ALT4) of each period, rumen fluid, blood, urine, and quarter milk (i.e., back right quarter) samples were collected at -0.5, 1, 2.5, 4, 5.5, and 7 h relative to morning feeding (0800 h).
34176630	9	53	theme	starch	1331:1336	arg1	levels					1350:1355	starch alternation levels	1331:1355	starch alternation levels at ALT4	1331:1363	Rumen medial pH was lower in the high alternation level compared with the low or medium alternation levels at ALT1 but did not differ among starch alternation levels at ALT4.
34176630	21	54	from	effects	3056:3062	arg1	health					3083:3088	health	3083:3088	health	3083:3088	This study indicates that cows can cope with day-to-day alternations in type of rumen fermentable organic matter; however, longer-term effects on performance and health should be addressed in future studies.
34176630	21	54	from	effects	3056:3062	arg1	performance					3067:3077	performance	3067:3077	performance	3067:3077	This study indicates that cows can cope with day-to-day alternations in type of rumen fermentable organic matter; however, longer-term effects on performance and health should be addressed in future studies.
34176630	19	55	theme	daily	2657:2661	arg1	alternation					2678:2688	the daily dietary starch alternation	2653:2688	the daily dietary starch alternation from 21% up to 42% (dry matter basis)	2653:2726	According to these results, it seems that the daily dietary starch alternation from 21% up to 42% (dry matter basis) is able to affect the ruminal fluid, especially during the first alternation.
34176630	19	55	theme	daily	2657:2661	arg1	able					2731:2734	able	2731:2734	able	2731:2734	According to these results, it seems that the daily dietary starch alternation from 21% up to 42% (dry matter basis) is able to affect the ruminal fluid, especially during the first alternation.
34176630	15	56	theme	alternation	2264:2274	arg1	level					2276:2280	the high alternation level	2255:2280	the high alternation level	2255:2280	However, glucose concentrations tended to be higher in cows from the high alternation level.
34176630	2	57	theme	dietary	404:410	arg1	starch					412:417	dietary starch	404:417	dietary starch	404:417	Six multiparous mid-lactation Holstein cows were used in a replicated 3 × 3 Latin square design with 14-d periods and 3 alternating levels of dietary starch as treatments.
34176630	2	58	theme	square	344:349	arg1	design					351:356	a replicated 3 × 3 Latin square design	319:356	a replicated 3 × 3 Latin square design with 14-d periods	319:374	Six multiparous mid-lactation Holstein cows were used in a replicated 3 × 3 Latin square design with 14-d periods and 3 alternating levels of dietary starch as treatments.
34176630	0	59	theme	dietary	25:31	arg1	level					40:44	the dietary starch level	21:44	the dietary starch level	21:44	Daily alternation of the dietary starch level in Holstein dairy cows.
34176630	1	60	theme	dairy	250:254	arg1	cows					256:259	dairy cows	250:259	dairy cows	250:259	The aim of this study was to investigate the effect of controlled daily alternations in dietary starch level on changes in rumen environment, blood, urine, and milk metabolites of dairy cows.
34176630	20	61	theme	rumen	2832:2836	arg1	fluid					2838:2842	rumen fluid	2832:2842	rumen fluid	2832:2842	However, these changes in rumen fluid did not cause any effect on the variables measured in blood, urine, or milk.
34176630	11	62	theme	morning	1652:1658	arg1	feeding					1660:1666	morning feeding	1652:1666	morning feeding	1652:1666	Total volatile fatty acid (VFA) concentrations were higher in the rumen medial fluid of the high alternation level at 7 h relative to morning feeding compared with those from the low and medium alternation levels.
34176630	6	63	contain	contained	796:804	arg1	diets					790:794	The 3 experimental diets	771:794	The 3 experimental diets	771:794	The 3 experimental diets contained 28% (low), 35% (medium), and 42% (high) starch (dry matter basis).
34176630	6	63	contain	contained	796:804	arg2	%					837:837	42%	835:837	42% (high)	835:844	The 3 experimental diets contained 28% (low), 35% (medium), and 42% (high) starch (dry matter basis).
34176630	6	63	contain	contained	796:804	arg2	%					819:819	35%	817:819	35% (medium)	817:828	The 3 experimental diets contained 28% (low), 35% (medium), and 42% (high) starch (dry matter basis).
34176630	6	63	contain	contained	796:804	arg2	medium					822:827	medium	822:827	medium	822:827	The 3 experimental diets contained 28% (low), 35% (medium), and 42% (high) starch (dry matter basis).
34176630	6	63	contain	contained	796:804	arg2	%					808:808	28%	806:808	28% (low)	806:814	The 3 experimental diets contained 28% (low), 35% (medium), and 42% (high) starch (dry matter basis).
34176630	0	64	theme	level	40:44	arg1	alternation					6:16	Daily alternation	0:16	Daily alternation of the dietary starch level in Holstein dairy cows.	0:68	Daily alternation of the dietary starch level in Holstein dairy cows.
34176630	21	65	theme	rumen	3001:3005	arg1	matter					3027:3032	rumen fermentable organic matter	3001:3032	rumen fermentable organic matter	3001:3032	This study indicates that cows can cope with day-to-day alternations in type of rumen fermentable organic matter; however, longer-term effects on performance and health should be addressed in future studies.
34176630	14	66	theme	starch	2163:2168	arg1	levels					2182:2187	starch alternation levels	2163:2187	starch alternation levels	2163:2187	No differences in blood pH were detected among starch alternation levels.
34176630	8	67	theme	matter	1133:1138	arg1	intake					1140:1145	dry matter intake	1129:1145	dry matter intake	1129:1145	No differences were observed in dry matter intake, milk yield, and milk chemical composition.
34176630	2	68	theme	mid-lactation	278:290	arg1	treatments					422:431	treatments	422:431	treatments	422:431	Six multiparous mid-lactation Holstein cows were used in a replicated 3 × 3 Latin square design with 14-d periods and 3 alternating levels of dietary starch as treatments.
34176630	2	68	theme	mid-lactation	278:290	arg1	cows					301:304	Six multiparous mid-lactation Holstein cows	262:304	Six multiparous mid-lactation Holstein cows	262:304	Six multiparous mid-lactation Holstein cows were used in a replicated 3 × 3 Latin square design with 14-d periods and 3 alternating levels of dietary starch as treatments.
34176630	7	69	theme	rumen	932:936	arg1	urine					952:956	urine	952:956	urine	952:956	At d 7 (baseline), 8 (ALT1), and 14 (ALT4) of each period, rumen fluid, blood, urine, and quarter milk (i.e., back right quarter) samples were collected at -0.5, 1, 2.5, 4, 5.5, and 7 h relative to morning feeding (0800 h).
34176630	7	69	theme	rumen	932:936	arg1	blood					945:949	blood	945:949	blood	945:949	At d 7 (baseline), 8 (ALT1), and 14 (ALT4) of each period, rumen fluid, blood, urine, and quarter milk (i.e., back right quarter) samples were collected at -0.5, 1, 2.5, 4, 5.5, and 7 h relative to morning feeding (0800 h).
34176630	7	69	theme	rumen	932:936	arg1	fluid					938:942	rumen fluid	932:942	rumen fluid	932:942	At d 7 (baseline), 8 (ALT1), and 14 (ALT4) of each period, rumen fluid, blood, urine, and quarter milk (i.e., back right quarter) samples were collected at -0.5, 1, 2.5, 4, 5.5, and 7 h relative to morning feeding (0800 h).
34176630	12	70	theme	relative	1844:1851	arg1	h					1842:1842	7 h	1840:1842	7 h relative to morning feeding	1840:1870	Similarly, total VFA concentrations constantly increased and were the highest in the ventral rumen fluid at 7 h relative to morning feeding, although no differences were detected among starch alternation levels.
34176630	21	71	theme	matter	3027:3032	arg1	type					2993:2996	type	2993:2996	type of rumen fermentable organic matter	2993:3032	This study indicates that cows can cope with day-to-day alternations in type of rumen fermentable organic matter; however, longer-term effects on performance and health should be addressed in future studies.
34176630	18	72	from	differences	2548:2558	arg1	pH					2568:2569	milk pH	2563:2569	milk pH	2563:2569	Similarly, no differences in milk pH were detected among alternation levels.
34176630	5	73	theme	experimental	728:739	arg1	diets					741:745	the 3 experimental diets	722:745	the 3 experimental diets on d 8, 10, 12, and 14	722:768	During the alternating period, the control diet was replaced with 1 of the 3 experimental diets on d 8, 10, 12, and 14.
34176630	9	74	from	ALT4	1360:1363	arg1	levels					1350:1355	starch alternation levels	1331:1355	starch alternation levels at ALT4	1331:1363	Rumen medial pH was lower in the high alternation level compared with the low or medium alternation levels at ALT1 but did not differ among starch alternation levels at ALT4.
34176630	7	75	theme	period	924:929	arg1	14					906:907	14	906:907	14	906:907	At d 7 (baseline), 8 (ALT1), and 14 (ALT4) of each period, rumen fluid, blood, urine, and quarter milk (i.e., back right quarter) samples were collected at -0.5, 1, 2.5, 4, 5.5, and 7 h relative to morning feeding (0800 h).
34176630	7	75	theme	period	924:929	arg1	period					924:929	each period	919:929	each period	919:929	At d 7 (baseline), 8 (ALT1), and 14 (ALT4) of each period, rumen fluid, blood, urine, and quarter milk (i.e., back right quarter) samples were collected at -0.5, 1, 2.5, 4, 5.5, and 7 h relative to morning feeding (0800 h).
34176630	7	75	theme	period	924:929	arg1	ALT4					910:913	ALT4	910:913	ALT4	910:913	At d 7 (baseline), 8 (ALT1), and 14 (ALT4) of each period, rumen fluid, blood, urine, and quarter milk (i.e., back right quarter) samples were collected at -0.5, 1, 2.5, 4, 5.5, and 7 h relative to morning feeding (0800 h).
34176630	12	76	theme	morning	1856:1862	arg1	feeding					1864:1870	morning feeding	1856:1870	morning feeding	1856:1870	Similarly, total VFA concentrations constantly increased and were the highest in the ventral rumen fluid at 7 h relative to morning feeding, although no differences were detected among starch alternation levels.
34176630	10	77	theme	ventral	1423:1429	arg1	contents					1431:1438	medial and ventral contents	1412:1438	medial and ventral contents	1412:1438	Similarly, the difference between rumen pH in medial and ventral contents was reduced at ALT1 with high alternation level but was not affected at ALT4.
34176630	13	78	theme	high	1989:1992	arg1	level					2006:2010	the high alternation level	1985:2010	the high alternation level	1985:2010	In both rumen medial and ventral fluids, the high alternation level showed higher propionate and lower acetate proportions compared with low and medium alternation levels.
34176630	12	79	theme	VFA	1749:1751	arg1	concentrations					1753:1766	total VFA concentrations	1743:1766	total VFA concentrations	1743:1766	Similarly, total VFA concentrations constantly increased and were the highest in the ventral rumen fluid at 7 h relative to morning feeding, although no differences were detected among starch alternation levels.
34176630	12	79	theme	VFA	1749:1751	arg1	highest					1802:1808	highest	1802:1808	highest	1802:1808	Similarly, total VFA concentrations constantly increased and were the highest in the ventral rumen fluid at 7 h relative to morning feeding, although no differences were detected among starch alternation levels.
34176630	9	80	theme	Rumen	1191:1195	arg1	pH					1204:1205	Rumen medial pH	1191:1205	Rumen medial pH	1191:1205	Rumen medial pH was lower in the high alternation level compared with the low or medium alternation levels at ALT1 but did not differ among starch alternation levels at ALT4.
34176630	10	81	theme	medial	1412:1417	arg1	contents					1431:1438	medial and ventral contents	1412:1438	medial and ventral contents	1412:1438	Similarly, the difference between rumen pH in medial and ventral contents was reduced at ALT1 with high alternation level but was not affected at ALT4.
34176630	16	82	theme	starch	2379:2384	arg1	level					2398:2402	the starch alternation level	2375:2402	the starch alternation level	2375:2402	l-Lactate concentrations in blood were higher in ALT1 than in ALT4 but were not affected by the starch alternation level.
34176630	3	83	theme	7-d	466:468	arg1	period					479:484	a 7-d baseline period	464:484	a 7-d baseline period	464:484	Each 14-d period consisted of a 7-d baseline period and 7-d alternating period where diets alternated day to day.
34176630	11	84	theme	acid	1539:1542	arg1	concentrations					1550:1563	Total volatile fatty acid (VFA) concentrations	1518:1563	Total volatile fatty acid (VFA) concentrations	1518:1563	Total volatile fatty acid (VFA) concentrations were higher in the rumen medial fluid of the high alternation level at 7 h relative to morning feeding compared with those from the low and medium alternation levels.
34176630	13	85	theme	higher	2019:2024	arg1	propionate					2026:2035	higher propionate	2019:2035	higher propionate	2019:2035	In both rumen medial and ventral fluids, the high alternation level showed higher propionate and lower acetate proportions compared with low and medium alternation levels.
34176630	8	86	theme	chemical	1169:1176	arg1	composition					1178:1188	milk chemical composition	1164:1188	milk chemical composition	1164:1188	No differences were observed in dry matter intake, milk yield, and milk chemical composition.
34176630	11	87	from	h	1638:1638	arg1	level					1627:1631	the high alternation level	1606:1631	the high alternation level at 7 h relative to morning feeding	1606:1666	Total volatile fatty acid (VFA) concentrations were higher in the rumen medial fluid of the high alternation level at 7 h relative to morning feeding compared with those from the low and medium alternation levels.
34176630	11	87	from	h	1638:1638	arg1	fluid					1597:1601	the rumen medial fluid	1580:1601	the rumen medial fluid of the high alternation level at 7 h relative to morning feeding	1580:1666	Total volatile fatty acid (VFA) concentrations were higher in the rumen medial fluid of the high alternation level at 7 h relative to morning feeding compared with those from the low and medium alternation levels.
34176630	13	88	theme	ventral	1969:1975	arg1	fluids					1977:1982	both rumen medial and ventral fluids	1947:1982	both rumen medial and ventral fluids	1947:1982	In both rumen medial and ventral fluids, the high alternation level showed higher propionate and lower acetate proportions compared with low and medium alternation levels.
34176630	1	89	theme	daily	136:140	arg1	alternations					142:153	controlled daily alternations	125:153	controlled daily alternations in dietary starch level	125:177	The aim of this study was to investigate the effect of controlled daily alternations in dietary starch level on changes in rumen environment, blood, urine, and milk metabolites of dairy cows.
34176630	13	90	theme	lower	2041:2045	arg1	acetate					2047:2053	lower acetate	2041:2053	lower acetate	2041:2053	In both rumen medial and ventral fluids, the high alternation level showed higher propionate and lower acetate proportions compared with low and medium alternation levels.
34176630	16	91	theme	l-Lactate	2283:2291	arg1	concentrations					2293:2306	l-Lactate concentrations	2283:2306	l-Lactate concentrations in blood	2283:2315	l-Lactate concentrations in blood were higher in ALT1 than in ALT4 but were not affected by the starch alternation level.
34176630	3	92	theme	7-d	490:492	arg1	period					506:511	7-d alternating period	490:511	7-d alternating period	490:511	Each 14-d period consisted of a 7-d baseline period and 7-d alternating period where diets alternated day to day.
34176630	5	93	theme	diets	741:745	arg1	diets					741:745	the 3 experimental diets	722:745	the 3 experimental diets on d 8, 10, 12, and 14	722:768	During the alternating period, the control diet was replaced with 1 of the 3 experimental diets on d 8, 10, 12, and 14.
34176630	5	93	theme	diets	741:745	arg1	1					717:717	1	717:717	1	717:717	During the alternating period, the control diet was replaced with 1 of the 3 experimental diets on d 8, 10, 12, and 14.
34176630	11	94	theme	alternation	1615:1625	arg1	level					1627:1631	the high alternation level	1606:1631	the high alternation level at 7 h relative to morning feeding	1606:1666	Total volatile fatty acid (VFA) concentrations were higher in the rumen medial fluid of the high alternation level at 7 h relative to morning feeding compared with those from the low and medium alternation levels.
34176630	14	95	from	differences	2119:2129	arg1	pH					2140:2141	blood pH	2134:2141	blood pH	2134:2141	No differences in blood pH were detected among starch alternation levels.
34176630	10	96	theme	alternation	1470:1480	arg1	level					1482:1486	high alternation level	1465:1486	high alternation level	1465:1486	Similarly, the difference between rumen pH in medial and ventral contents was reduced at ALT1 with high alternation level but was not affected at ALT4.
34176630	11	97	theme	volatile	1524:1531	arg1	concentrations					1550:1563	Total volatile fatty acid (VFA) concentrations	1518:1563	Total volatile fatty acid (VFA) concentrations	1518:1563	Total volatile fatty acid (VFA) concentrations were higher in the rumen medial fluid of the high alternation level at 7 h relative to morning feeding compared with those from the low and medium alternation levels.
34176630	17	98	from	differences	2418:2428	arg1	pH					2433:2434	pH	2433:2434	pH	2433:2434	In urine, no differences in pH or l-lactate concentrations were detected among alternation levels (i.e., low, medium, and high).
34176630	17	98	from	differences	2418:2428	arg1	concentrations					2449:2462	l-lactate concentrations	2439:2462	l-lactate concentrations	2439:2462	In urine, no differences in pH or l-lactate concentrations were detected among alternation levels (i.e., low, medium, and high).
34176630	1	99	theme	starch	166:171	arg1	level					173:177	dietary starch level	158:177	dietary starch level	158:177	The aim of this study was to investigate the effect of controlled daily alternations in dietary starch level on changes in rumen environment, blood, urine, and milk metabolites of dairy cows.
34176630	9	100	theme	alternation	1229:1239	arg1	level					1241:1245	the high alternation level	1220:1245	the high alternation level	1220:1245	Rumen medial pH was lower in the high alternation level compared with the low or medium alternation levels at ALT1 but did not differ among starch alternation levels at ALT4.
34176630	17	101	dep	levels	2496:2501	arg1	medium					2515:2520	medium	2515:2520	medium	2515:2520	In urine, no differences in pH or l-lactate concentrations were detected among alternation levels (i.e., low, medium, and high).
34176630	17	101	dep	levels	2496:2501	arg1	high					2527:2530	high	2527:2530	high	2527:2530	In urine, no differences in pH or l-lactate concentrations were detected among alternation levels (i.e., low, medium, and high).
34176630	17	101	dep	levels	2496:2501	arg1	low					2510:2512	low	2510:2512	low	2510:2512	In urine, no differences in pH or l-lactate concentrations were detected among alternation levels (i.e., low, medium, and high).
34176630	19	102	theme	starch	2671:2676	arg1	alternation					2678:2688	the daily dietary starch alternation	2653:2688	the daily dietary starch alternation from 21% up to 42% (dry matter basis)	2653:2726	According to these results, it seems that the daily dietary starch alternation from 21% up to 42% (dry matter basis) is able to affect the ruminal fluid, especially during the first alternation.
34176630	19	102	theme	starch	2671:2676	arg1	able					2731:2734	able	2731:2734	able	2731:2734	According to these results, it seems that the daily dietary starch alternation from 21% up to 42% (dry matter basis) is able to affect the ruminal fluid, especially during the first alternation.
34176630	13	103	theme	medial	1958:1963	arg1	fluids					1977:1982	both rumen medial and ventral fluids	1947:1982	both rumen medial and ventral fluids	1947:1982	In both rumen medial and ventral fluids, the high alternation level showed higher propionate and lower acetate proportions compared with low and medium alternation levels.
34176630	12	104	theme	rumen	1825:1829	arg1	fluid					1831:1835	the ventral rumen fluid	1813:1835	the ventral rumen fluid	1813:1835	Similarly, total VFA concentrations constantly increased and were the highest in the ventral rumen fluid at 7 h relative to morning feeding, although no differences were detected among starch alternation levels.
34176630	4	105	theme	baseline	559:566	arg1	period					568:573	the baseline period	555:573	the baseline period	555:573	During the baseline period, all cows were fed a control diet containing 21% starch (dry matter basis).
34176630	11	106	from	fluid	1597:1601	arg1	h					1638:1638	7 h	1636:1638	7 h	1636:1638	Total volatile fatty acid (VFA) concentrations were higher in the rumen medial fluid of the high alternation level at 7 h relative to morning feeding compared with those from the low and medium alternation levels.
34176630	1	107	from	changes	182:188	arg1	environment					199:209	rumen environment	193:209	rumen environment	193:209	The aim of this study was to investigate the effect of controlled daily alternations in dietary starch level on changes in rumen environment, blood, urine, and milk metabolites of dairy cows.
34176630	1	107	from	changes	182:188	arg1	metabolites					235:245	milk metabolites	230:245	milk metabolites of dairy cows	230:259	The aim of this study was to investigate the effect of controlled daily alternations in dietary starch level on changes in rumen environment, blood, urine, and milk metabolites of dairy cows.
34176630	1	107	from	changes	182:188	arg1	urine					219:223	urine	219:223	urine	219:223	The aim of this study was to investigate the effect of controlled daily alternations in dietary starch level on changes in rumen environment, blood, urine, and milk metabolites of dairy cows.
34176630	1	107	from	changes	182:188	arg1	blood					212:216	blood	212:216	blood	212:216	The aim of this study was to investigate the effect of controlled daily alternations in dietary starch level on changes in rumen environment, blood, urine, and milk metabolites of dairy cows.
34176630	1	108	theme	study	86:90	arg1	aim					74:76	The aim	70:76	The aim of this study	70:90	The aim of this study was to investigate the effect of controlled daily alternations in dietary starch level on changes in rumen environment, blood, urine, and milk metabolites of dairy cows.
34176630	11	109	theme	rumen	1584:1588	arg1	fluid					1597:1601	the rumen medial fluid	1580:1601	the rumen medial fluid of the high alternation level at 7 h relative to morning feeding	1580:1666	Total volatile fatty acid (VFA) concentrations were higher in the rumen medial fluid of the high alternation level at 7 h relative to morning feeding compared with those from the low and medium alternation levels.
34176630	1	110	from	level	173:177	arg1	effect					115:120	the effect	111:120	the effect of controlled daily alternations in dietary starch level on changes in rumen environment, blood, urine, and milk metabolites of dairy cows	111:259	The aim of this study was to investigate the effect of controlled daily alternations in dietary starch level on changes in rumen environment, blood, urine, and milk metabolites of dairy cows.
34176630	7	111	dep	quarter	994:1000	arg1	i.e.					977:980	i.e.	977:980	i.e.	977:980	At d 7 (baseline), 8 (ALT1), and 14 (ALT4) of each period, rumen fluid, blood, urine, and quarter milk (i.e., back right quarter) samples were collected at -0.5, 1, 2.5, 4, 5.5, and 7 h relative to morning feeding (0800 h).
34176630	7	112	theme	right	988:992	arg1	quarter					994:1000	back right quarter	983:1000	back right quarter	983:1000	At d 7 (baseline), 8 (ALT1), and 14 (ALT4) of each period, rumen fluid, blood, urine, and quarter milk (i.e., back right quarter) samples were collected at -0.5, 1, 2.5, 4, 5.5, and 7 h relative to morning feeding (0800 h).
34176630	4	113	theme	%	622:622	arg1	basis					643:647	dry matter basis	632:647	dry matter basis	632:647	During the baseline period, all cows were fed a control diet containing 21% starch (dry matter basis).
34176630	4	113	theme	%	622:622	arg1	starch					624:629	21% starch	620:629	21% starch (dry matter basis)	620:648	During the baseline period, all cows were fed a control diet containing 21% starch (dry matter basis).
34176630	19	114	theme	dry	2710:2712	arg1	basis					2721:2725	dry matter basis	2710:2725	dry matter basis	2710:2725	According to these results, it seems that the daily dietary starch alternation from 21% up to 42% (dry matter basis) is able to affect the ruminal fluid, especially during the first alternation.
34176630	19	114	theme	dry	2710:2712	arg1	%					2697:2697	21%	2695:2697	21% up to 42% (dry matter basis)	2695:2726	According to these results, it seems that the daily dietary starch alternation from 21% up to 42% (dry matter basis) is able to affect the ruminal fluid, especially during the first alternation.
34176630	0	115	theme	dairy	58:62	arg1	cows					64:67	Holstein dairy cows	49:67	Holstein dairy cows	49:67	Daily alternation of the dietary starch level in Holstein dairy cows.
34176630	21	116	theme	future	3113:3118	arg1	studies					3120:3126	future studies	3113:3126	future studies	3113:3126	This study indicates that cows can cope with day-to-day alternations in type of rumen fermentable organic matter; however, longer-term effects on performance and health should be addressed in future studies.
34176630	13	117	dep	propionate	2026:2035	arg1	proportions					2055:2065	proportions	2055:2065	proportions	2055:2065	In both rumen medial and ventral fluids, the high alternation level showed higher propionate and lower acetate proportions compared with low and medium alternation levels.
34176630	9	118	theme	alternation	1279:1289	arg1	levels					1291:1296	the low or medium alternation levels	1261:1296	the low or medium alternation levels	1261:1296	Rumen medial pH was lower in the high alternation level compared with the low or medium alternation levels at ALT1 but did not differ among starch alternation levels at ALT4.
34176630	20	119	from	changes	2821:2827	arg1	fluid					2838:2842	rumen fluid	2832:2842	rumen fluid	2832:2842	However, these changes in rumen fluid did not cause any effect on the variables measured in blood, urine, or milk.
34176630	15	120	from	level	2276:2280	arg1	cows					2245:2248	cows	2245:2248	cows from the high alternation level	2245:2280	However, glucose concentrations tended to be higher in cows from the high alternation level.
34176630	19	121	theme	dietary	2663:2669	arg1	alternation					2678:2688	the daily dietary starch alternation	2653:2688	the daily dietary starch alternation from 21% up to 42% (dry matter basis)	2653:2726	According to these results, it seems that the daily dietary starch alternation from 21% up to 42% (dry matter basis) is able to affect the ruminal fluid, especially during the first alternation.
34176630	19	121	theme	dietary	2663:2669	arg1	able					2731:2734	able	2731:2734	able	2731:2734	According to these results, it seems that the daily dietary starch alternation from 21% up to 42% (dry matter basis) is able to affect the ruminal fluid, especially during the first alternation.
34176630	1	122	from	alternations	142:153	arg1	level					173:177	dietary starch level	158:177	dietary starch level	158:177	The aim of this study was to investigate the effect of controlled daily alternations in dietary starch level on changes in rumen environment, blood, urine, and milk metabolites of dairy cows.
34176630	2	123	theme	starch	412:417	arg1	levels					394:399	3 alternating levels	380:399	3 alternating levels of dietary starch	380:417	Six multiparous mid-lactation Holstein cows were used in a replicated 3 × 3 Latin square design with 14-d periods and 3 alternating levels of dietary starch as treatments.
34176630	2	123	theme	starch	412:417	arg1	design					351:356	a replicated 3 × 3 Latin square design	319:356	a replicated 3 × 3 Latin square design with 14-d periods	319:374	Six multiparous mid-lactation Holstein cows were used in a replicated 3 × 3 Latin square design with 14-d periods and 3 alternating levels of dietary starch as treatments.
34176630	0	124	from	alternation	6:16	arg1	cows					64:67	Holstein dairy cows	49:67	Holstein dairy cows	49:67	Daily alternation of the dietary starch level in Holstein dairy cows.
34176630	19	125	theme	ruminal	2750:2756	arg1	fluid					2758:2762	the ruminal fluid	2746:2762	the ruminal fluid	2746:2762	According to these results, it seems that the daily dietary starch alternation from 21% up to 42% (dry matter basis) is able to affect the ruminal fluid, especially during the first alternation.
34176630	4	126	theme	control	596:602	arg1	diet					604:607	a control diet	594:607	a control diet containing 21% starch (dry matter basis)	594:648	During the baseline period, all cows were fed a control diet containing 21% starch (dry matter basis).
34176630	13	127	theme	low	2081:2083	arg1	levels					2108:2113	low and medium alternation levels	2081:2113	low and medium alternation levels	2081:2113	In both rumen medial and ventral fluids, the high alternation level showed higher propionate and lower acetate proportions compared with low and medium alternation levels.
34176630	20	128	from	effect	2862:2867	arg1	variables					2876:2884	the variables	2872:2884	the variables measured in blood, urine, or milk	2872:2918	However, these changes in rumen fluid did not cause any effect on the variables measured in blood, urine, or milk.
34176630	6	129	theme	matter	858:863	arg1	basis					865:869	dry matter basis	854:869	dry matter basis	854:869	The 3 experimental diets contained 28% (low), 35% (medium), and 42% (high) starch (dry matter basis).
34176630	6	129	theme	matter	858:863	arg1	starch					846:851	starch	846:851	starch	846:851	The 3 experimental diets contained 28% (low), 35% (medium), and 42% (high) starch (dry matter basis).
34176630	13	130	theme	medium	2089:2094	arg1	alternation					2096:2106	medium alternation	2089:2106	medium alternation	2089:2106	In both rumen medial and ventral fluids, the high alternation level showed higher propionate and lower acetate proportions compared with low and medium alternation levels.
34176630	19	131	dep	%	2697:2697	arg1	%					2707:2707	up to 42%	2699:2707	21% up to 42% (dry matter basis)	2695:2726	According to these results, it seems that the daily dietary starch alternation from 21% up to 42% (dry matter basis) is able to affect the ruminal fluid, especially during the first alternation.
34176630	1	132	from	effect	115:120	arg1	level					173:177	dietary starch level	158:177	dietary starch level	158:177	The aim of this study was to investigate the effect of controlled daily alternations in dietary starch level on changes in rumen environment, blood, urine, and milk metabolites of dairy cows.
34176630	1	132	from	effect	115:120	arg1	changes					182:188	changes	182:188	changes in rumen environment, blood, urine, and milk metabolites of dairy cows	182:259	The aim of this study was to investigate the effect of controlled daily alternations in dietary starch level on changes in rumen environment, blood, urine, and milk metabolites of dairy cows.
34176630	12	133	theme	total	1743:1747	arg1	concentrations					1753:1766	total VFA concentrations	1743:1766	total VFA concentrations	1743:1766	Similarly, total VFA concentrations constantly increased and were the highest in the ventral rumen fluid at 7 h relative to morning feeding, although no differences were detected among starch alternation levels.
34176630	12	133	theme	total	1743:1747	arg1	highest					1802:1808	highest	1802:1808	highest	1802:1808	Similarly, total VFA concentrations constantly increased and were the highest in the ventral rumen fluid at 7 h relative to morning feeding, although no differences were detected among starch alternation levels.
34176630	2	134	theme	×	334:334	arg1	design					351:356	a replicated 3 × 3 Latin square design	319:356	a replicated 3 × 3 Latin square design with 14-d periods	319:374	Six multiparous mid-lactation Holstein cows were used in a replicated 3 × 3 Latin square design with 14-d periods and 3 alternating levels of dietary starch as treatments.
34176630	5	135	theme	control	686:692	arg1	diet					694:697	the control diet	682:697	the control diet	682:697	During the alternating period, the control diet was replaced with 1 of the 3 experimental diets on d 8, 10, 12, and 14.
34176630	17	136	located	detected	2469:2476	arg1	urine					2408:2412	urine	2408:2412	urine	2408:2412	In urine, no differences in pH or l-lactate concentrations were detected among alternation levels (i.e., low, medium, and high).
34176630	17	136	located	detected	2469:2476	arg2	differences					2418:2428	no differences	2415:2428	no differences in pH or l-lactate concentrations	2415:2462	In urine, no differences in pH or l-lactate concentrations were detected among alternation levels (i.e., low, medium, and high).
34176630	2	137	theme	replicated	321:330	arg1	design					351:356	a replicated 3 × 3 Latin square design	319:356	a replicated 3 × 3 Latin square design with 14-d periods	319:374	Six multiparous mid-lactation Holstein cows were used in a replicated 3 × 3 Latin square design with 14-d periods and 3 alternating levels of dietary starch as treatments.
34176630	15	138	theme	glucose	2199:2205	arg1	concentrations					2207:2220	glucose concentrations	2199:2220	glucose concentrations	2199:2220	However, glucose concentrations tended to be higher in cows from the high alternation level.
34176630	1	139	theme	milk	230:233	arg1	metabolites					235:245	milk metabolites	230:245	milk metabolites of dairy cows	230:259	The aim of this study was to investigate the effect of controlled daily alternations in dietary starch level on changes in rumen environment, blood, urine, and milk metabolites of dairy cows.
34176630	2	140	theme	14-d	363:366	arg1	periods					368:374	14-d periods	363:374	14-d periods	363:374	Six multiparous mid-lactation Holstein cows were used in a replicated 3 × 3 Latin square design with 14-d periods and 3 alternating levels of dietary starch as treatments.
34176630	12	141	theme	starch	1917:1922	arg1	levels					1936:1941	starch alternation levels	1917:1941	starch alternation levels	1917:1941	Similarly, total VFA concentrations constantly increased and were the highest in the ventral rumen fluid at 7 h relative to morning feeding, although no differences were detected among starch alternation levels.
34176630	4	142	theme	matter	636:641	arg1	basis					643:647	dry matter basis	632:647	dry matter basis	632:647	During the baseline period, all cows were fed a control diet containing 21% starch (dry matter basis).
34176630	4	142	theme	matter	636:641	arg1	starch					624:629	21% starch	620:629	21% starch (dry matter basis)	620:648	During the baseline period, all cows were fed a control diet containing 21% starch (dry matter basis).
34176630	0	143	theme	starch	33:38	arg1	level					40:44	the dietary starch level	21:44	the dietary starch level	21:44	Daily alternation of the dietary starch level in Holstein dairy cows.
34176630	17	144	dep	low	2510:2512	arg1	i.e.					2504:2507	i.e., low, medium, and high	2504:2530	i.e.	2504:2507	In urine, no differences in pH or l-lactate concentrations were detected among alternation levels (i.e., low, medium, and high).
34176630	18	145	theme	alternation	2591:2601	arg1	levels					2603:2608	alternation levels	2591:2608	alternation levels	2591:2608	Similarly, no differences in milk pH were detected among alternation levels.
34176630	8	146	theme	dry	1129:1131	arg1	intake					1140:1145	dry matter intake	1129:1145	dry matter intake	1129:1145	No differences were observed in dry matter intake, milk yield, and milk chemical composition.
34176630	11	147	dep	low	1697:1699	arg1	levels					1724:1729	alternation levels	1712:1729	alternation levels	1712:1729	Total volatile fatty acid (VFA) concentrations were higher in the rumen medial fluid of the high alternation level at 7 h relative to morning feeding compared with those from the low and medium alternation levels.
34176630	1	148	theme	cows	256:259	arg1	environment					199:209	rumen environment	193:209	rumen environment	193:209	The aim of this study was to investigate the effect of controlled daily alternations in dietary starch level on changes in rumen environment, blood, urine, and milk metabolites of dairy cows.
34176630	1	148	theme	cows	256:259	arg1	metabolites					235:245	milk metabolites	230:245	milk metabolites of dairy cows	230:259	The aim of this study was to investigate the effect of controlled daily alternations in dietary starch level on changes in rumen environment, blood, urine, and milk metabolites of dairy cows.
34176630	1	148	theme	cows	256:259	arg1	urine					219:223	urine	219:223	urine	219:223	The aim of this study was to investigate the effect of controlled daily alternations in dietary starch level on changes in rumen environment, blood, urine, and milk metabolites of dairy cows.
34176630	1	148	theme	cows	256:259	arg1	blood					212:216	blood	212:216	blood	212:216	The aim of this study was to investigate the effect of controlled daily alternations in dietary starch level on changes in rumen environment, blood, urine, and milk metabolites of dairy cows.
34176630	14	149	theme	alternation	2170:2180	arg1	levels					2182:2187	starch alternation levels	2163:2187	starch alternation levels	2163:2187	No differences in blood pH were detected among starch alternation levels.
34176630	2	150	theme	alternating	382:392	arg1	levels					394:399	3 alternating levels	380:399	3 alternating levels of dietary starch	380:417	Six multiparous mid-lactation Holstein cows were used in a replicated 3 × 3 Latin square design with 14-d periods and 3 alternating levels of dietary starch as treatments.
34176630	2	151	theme	Holstein	292:299	arg1	treatments					422:431	treatments	422:431	treatments	422:431	Six multiparous mid-lactation Holstein cows were used in a replicated 3 × 3 Latin square design with 14-d periods and 3 alternating levels of dietary starch as treatments.
34176630	2	151	theme	Holstein	292:299	arg1	cows					301:304	Six multiparous mid-lactation Holstein cows	262:304	Six multiparous mid-lactation Holstein cows	262:304	Six multiparous mid-lactation Holstein cows were used in a replicated 3 × 3 Latin square design with 14-d periods and 3 alternating levels of dietary starch as treatments.
34176630	16	152	from	concentrations	2293:2306	arg1	blood					2311:2315	blood	2311:2315	blood	2311:2315	l-Lactate concentrations in blood were higher in ALT1 than in ALT4 but were not affected by the starch alternation level.
34176630	21	153	theme	fermentable	3007:3017	arg1	matter					3027:3032	rumen fermentable organic matter	3001:3032	rumen fermentable organic matter	3001:3032	This study indicates that cows can cope with day-to-day alternations in type of rumen fermentable organic matter; however, longer-term effects on performance and health should be addressed in future studies.
34176630	8	154	located	observed	1117:1124	arg1	yield					1153:1157	milk yield	1148:1157	milk yield	1148:1157	No differences were observed in dry matter intake, milk yield, and milk chemical composition.
34176630	8	154	located	observed	1117:1124	arg1	intake					1140:1145	dry matter intake	1129:1145	dry matter intake	1129:1145	No differences were observed in dry matter intake, milk yield, and milk chemical composition.
34176630	8	154	located	observed	1117:1124	arg2	differences					1100:1110	No differences	1097:1110	No differences	1097:1110	No differences were observed in dry matter intake, milk yield, and milk chemical composition.
34176630	8	154	located	observed	1117:1124	arg1	composition					1178:1188	milk chemical composition	1164:1188	milk chemical composition	1164:1188	No differences were observed in dry matter intake, milk yield, and milk chemical composition.
34176630	8	155	theme	milk	1148:1151	arg1	yield					1153:1157	milk yield	1148:1157	milk yield	1148:1157	No differences were observed in dry matter intake, milk yield, and milk chemical composition.
34176630	10	156	theme	rumen	1400:1404	arg1	pH					1406:1407	rumen pH	1400:1407	rumen pH	1400:1407	Similarly, the difference between rumen pH in medial and ventral contents was reduced at ALT1 with high alternation level but was not affected at ALT4.
34176630	2	157	theme	multiparous	266:276	arg1	treatments					422:431	treatments	422:431	treatments	422:431	Six multiparous mid-lactation Holstein cows were used in a replicated 3 × 3 Latin square design with 14-d periods and 3 alternating levels of dietary starch as treatments.
34176630	2	157	theme	multiparous	266:276	arg1	cows					301:304	Six multiparous mid-lactation Holstein cows	262:304	Six multiparous mid-lactation Holstein cows	262:304	Six multiparous mid-lactation Holstein cows were used in a replicated 3 × 3 Latin square design with 14-d periods and 3 alternating levels of dietary starch as treatments.
34176630	1	158	theme	rumen	193:197	arg1	environment					199:209	rumen environment	193:209	rumen environment	193:209	The aim of this study was to investigate the effect of controlled daily alternations in dietary starch level on changes in rumen environment, blood, urine, and milk metabolites of dairy cows.
34176630	7	159	theme	morning	1071:1077	arg1	feeding					1079:1085	morning feeding	1071:1085	morning feeding (0800 h)	1071:1094	At d 7 (baseline), 8 (ALT1), and 14 (ALT4) of each period, rumen fluid, blood, urine, and quarter milk (i.e., back right quarter) samples were collected at -0.5, 1, 2.5, 4, 5.5, and 7 h relative to morning feeding (0800 h).
34176630	7	159	theme	morning	1071:1077	arg1	h					1093:1093	0800 h	1088:1093	0800 h	1088:1093	At d 7 (baseline), 8 (ALT1), and 14 (ALT4) of each period, rumen fluid, blood, urine, and quarter milk (i.e., back right quarter) samples were collected at -0.5, 1, 2.5, 4, 5.5, and 7 h relative to morning feeding (0800 h).
34176630	21	160	dep	cope	2956:2959	arg1	addressed					3100:3108	addressed	3100:3108	should be addressed in future studies	3090:3126	This study indicates that cows can cope with day-to-day alternations in type of rumen fermentable organic matter; however, longer-term effects on performance and health should be addressed in future studies.
34176630	6	161	theme	dry	854:856	arg1	basis					865:869	dry matter basis	854:869	dry matter basis	854:869	The 3 experimental diets contained 28% (low), 35% (medium), and 42% (high) starch (dry matter basis).
34176630	6	161	theme	dry	854:856	arg1	starch					846:851	starch	846:851	starch	846:851	The 3 experimental diets contained 28% (low), 35% (medium), and 42% (high) starch (dry matter basis).
34176630	19	162	theme	first	2787:2791	arg1	alternation					2793:2803	the first alternation	2783:2803	the first alternation	2783:2803	According to these results, it seems that the daily dietary starch alternation from 21% up to 42% (dry matter basis) is able to affect the ruminal fluid, especially during the first alternation.
32738624	2	0	theme	cold-acclimation	362:377	arg1	responses					379:387	protective cold-acclimation responses	351:387	protective cold-acclimation responses of organisms	351:400	Toxic metals in the environment can interfere with protective cold-acclimation responses of organisms.
32738624	1	1	theme	geographic	246:255	arg1	distribution					257:268	geographic distribution	246:268	geographic distribution	246:268	Freezing temperatures is an important stressor in the arctic regions and has a significant influence on the population dynamics and geographic distribution of terrestrial invertebrates.
32738624	4	2	theme	freeze-tolerance	601:616	arg1	aspects					590:596	various physiological aspects	568:596	various physiological aspects of freeze-tolerance in the earthworm (Enchytraeus albidus)	568:655	Here, we aimed to investigate the effects of Hg (HgCl2) on various physiological aspects of freeze-tolerance in the earthworm (Enchytraeus albidus).
32738624	1	3	theme	arctic	168:173	arg1	regions					175:181	the arctic regions	164:181	the arctic regions	164:181	Freezing temperatures is an important stressor in the arctic regions and has a significant influence on the population dynamics and geographic distribution of terrestrial invertebrates.
32738624	7	4	theme	exposure	1322:1329	arg1	times					1331:1335	prolonged exposure times	1312:1335	prolonged exposure times	1312:1335	Thus, the LC50 value in frozen soil decreased from 8.3 mg Hg kg-1 (when exposed for 17 days) to only 4.2 mg Hg kg-1 after 36 days' exposure indicating that combined effects of Hg and freezing became larger at prolonged exposure times.
32738624	10	5	theme	negative	1834:1841	arg1	related					1893:1899	related	1893:1899	related	1893:1899	Since a high proportion of unsaturated fatty acids in the membrane is important for invertebrate freeze-tolerance, our results suggest that the negative effect of Hg on freeze-tolerance in E. albidus is related to degraded membrane functionality at low temperature.
32738624	10	5	theme	negative	1834:1841	arg1	effect					1843:1848	the negative effect	1830:1848	the negative effect of Hg on freeze-tolerance in E. albidus	1830:1888	Since a high proportion of unsaturated fatty acids in the membrane is important for invertebrate freeze-tolerance, our results suggest that the negative effect of Hg on freeze-tolerance in E. albidus is related to degraded membrane functionality at low temperature.
32738624	9	6	theme	clear	1575:1579	arg1	effects					1590:1596	clear negative effects	1575:1596	clear negative effects	1575:1596	Hg had clear negative effects on the proportion of unsaturated PLFAs, which could be an indication of lipid peroxidation.
32738624	1	7	dep	dynamics	233:240	arg1	the					218:220	the	218:220	the	218:220	Freezing temperatures is an important stressor in the arctic regions and has a significant influence on the population dynamics and geographic distribution of terrestrial invertebrates.
32738624	5	8	from	content	725:731	arg1	composition					826:836	the composition	822:836	the composition of membrane phospholipid fatty acids (PLFA)	822:880	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	5	9	theme	membrane	841:848	arg1	PLFA					876:879	PLFA	876:879	PLFA	876:879	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	5	9	theme	membrane	841:848	arg1	acids					869:873	membrane phospholipid fatty acids	841:873	membrane phospholipid fatty acids (PLFA)	841:880	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	6	10	theme	at	930:931	arg1	-6 °C					933:937	Freezing at -6 °C	921:937	Freezing at -6 °C	921:937	Freezing at -6 °C had no effect on survival in uncontaminated soil, however, survival of freezing in Hg contaminated soil was clearly reduced, especially at extended exposure times.
32738624	8	11	theme	glycogen	1363:1370	arg1	reserves					1372:1379	glycogen reserves	1363:1379	glycogen reserves (almost 50% at 12 mg kg-1 dry soil)	1363:1415	Hg caused a depletion of glycogen reserves (almost 50% at 12 mg kg-1 dry soil), but despite this effect worms were able to maintain a constant cryoprotectant level (about 0.12 mg glucose mg-1 dry weight) at all Hg concentrations.
32738624	1	12	theme	Freezing	114:121	arg1	temperatures					123:134	Freezing temperatures	114:134	Freezing temperatures	114:134	Freezing temperatures is an important stressor in the arctic regions and has a significant influence on the population dynamics and geographic distribution of terrestrial invertebrates.
32738624	1	12	theme	Freezing	114:121	arg1	stressor					152:159	an important stressor	139:159	an important stressor in the arctic regions	139:181	Freezing temperatures is an important stressor in the arctic regions and has a significant influence on the population dynamics and geographic distribution of terrestrial invertebrates.
32738624	5	13	theme	glucose	703:709	arg1	content					725:731	the glycogen content	712:731	the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism)	712:804	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	5	13	theme	glucose	703:709	arg1	changes					811:817	changes	811:817	changes in the composition of membrane phospholipid fatty acids (PLFA)	811:880	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	5	13	theme	glucose	703:709	arg1	indicator					888:896	an indicator	885:896	an indicator of lipid peroxidation	885:918	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	5	13	theme	glucose	703:709	arg1	levels					674:679	the levels	670:679	the levels of the cryoprotectant glucose	670:709	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	5	14	theme	peroxidation	907:918	arg1	content					725:731	the glycogen content	712:731	the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism)	712:804	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	5	14	theme	peroxidation	907:918	arg1	changes					811:817	changes	811:817	changes in the composition of membrane phospholipid fatty acids (PLFA)	811:880	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	5	14	theme	peroxidation	907:918	arg1	indicator					888:896	an indicator	885:896	an indicator of lipid peroxidation	885:918	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	5	14	theme	peroxidation	907:918	arg1	levels					674:679	the levels	670:679	the levels of the cryoprotectant glucose	670:709	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	7	15	theme	combined	1259:1266	arg1	effects					1268:1274	combined effects	1259:1274	combined effects of Hg and freezing	1259:1293	Thus, the LC50 value in frozen soil decreased from 8.3 mg Hg kg-1 (when exposed for 17 days) to only 4.2 mg Hg kg-1 after 36 days' exposure indicating that combined effects of Hg and freezing became larger at prolonged exposure times.
32738624	8	16	theme	mg-1	1525:1528	arg1	level					1496:1500	a constant cryoprotectant level	1470:1500	a constant cryoprotectant level (about 0.12 mg glucose mg-1 dry weight)	1470:1540	Hg caused a depletion of glycogen reserves (almost 50% at 12 mg kg-1 dry soil), but despite this effect worms were able to maintain a constant cryoprotectant level (about 0.12 mg glucose mg-1 dry weight) at all Hg concentrations.
32738624	8	16	theme	mg-1	1525:1528	arg1	weight					1534:1539	about 0.12 mg glucose mg-1 dry weight	1503:1539	about 0.12 mg glucose mg-1 dry weight	1503:1539	Hg caused a depletion of glycogen reserves (almost 50% at 12 mg kg-1 dry soil), but despite this effect worms were able to maintain a constant cryoprotectant level (about 0.12 mg glucose mg-1 dry weight) at all Hg concentrations.
32738624	1	17	theme	important	142:150	arg1	temperatures					123:134	Freezing temperatures	114:134	Freezing temperatures	114:134	Freezing temperatures is an important stressor in the arctic regions and has a significant influence on the population dynamics and geographic distribution of terrestrial invertebrates.
32738624	1	17	theme	important	142:150	arg1	stressor					152:159	an important stressor	139:159	an important stressor in the arctic regions	139:181	Freezing temperatures is an important stressor in the arctic regions and has a significant influence on the population dynamics and geographic distribution of terrestrial invertebrates.
32738624	9	18	theme	PLFAs	1631:1635	arg1	proportion					1605:1614	the proportion	1601:1614	the proportion of unsaturated PLFAs, which could be an indication of lipid peroxidation	1601:1687	Hg had clear negative effects on the proportion of unsaturated PLFAs, which could be an indication of lipid peroxidation.
32738624	10	19	theme	degraded	1904:1911	arg1	functionality					1922:1934	degraded membrane functionality	1904:1934	degraded membrane functionality	1904:1934	Since a high proportion of unsaturated fatty acids in the membrane is important for invertebrate freeze-tolerance, our results suggest that the negative effect of Hg on freeze-tolerance in E. albidus is related to degraded membrane functionality at low temperature.
32738624	1	20	from	stressor	152:159	arg1	regions					175:181	the arctic regions	164:181	the arctic regions	164:181	Freezing temperatures is an important stressor in the arctic regions and has a significant influence on the population dynamics and geographic distribution of terrestrial invertebrates.
32738624	6	21	theme	uncontaminated	968:981	arg1	soil					983:986	uncontaminated soil	968:986	uncontaminated soil	968:986	Freezing at -6 °C had no effect on survival in uncontaminated soil, however, survival of freezing in Hg contaminated soil was clearly reduced, especially at extended exposure times.
32738624	8	22	theme	dry	1407:1409	arg1	soil					1411:1414	12 mg kg-1 dry soil	1396:1414	12 mg kg-1 dry soil	1396:1414	Hg caused a depletion of glycogen reserves (almost 50% at 12 mg kg-1 dry soil), but despite this effect worms were able to maintain a constant cryoprotectant level (about 0.12 mg glucose mg-1 dry weight) at all Hg concentrations.
32738624	6	23	from	survival	998:1005	arg1	soil					1038:1041	Hg contaminated soil	1022:1041	Hg contaminated soil	1022:1041	Freezing at -6 °C had no effect on survival in uncontaminated soil, however, survival of freezing in Hg contaminated soil was clearly reduced, especially at extended exposure times.
32738624	0	24	theme	arctic	76:81	arg1	albidus					105:111	the arctic earthworm Enchytraeus albidus	72:111	the arctic earthworm Enchytraeus albidus	72:111	Mercury (Hg2+) interferes with physiological adaptations to freezing in the arctic earthworm Enchytraeus albidus.
32738624	5	25	from	changes	811:817	arg1	composition					826:836	the composition	822:836	the composition of membrane phospholipid fatty acids (PLFA)	822:880	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	0	26	theme	Enchytraeus	93:103	arg1	albidus					105:111	the arctic earthworm Enchytraeus albidus	72:111	the arctic earthworm Enchytraeus albidus	72:111	Mercury (Hg2+) interferes with physiological adaptations to freezing in the arctic earthworm Enchytraeus albidus.
32738624	10	27	from	effect	1843:1848	arg1	freeze-tolerance					1859:1874	freeze-tolerance	1859:1874	freeze-tolerance in E. albidus	1859:1888	Since a high proportion of unsaturated fatty acids in the membrane is important for invertebrate freeze-tolerance, our results suggest that the negative effect of Hg on freeze-tolerance in E. albidus is related to degraded membrane functionality at low temperature.
32738624	3	28	theme	combined	445:452	arg1	effects					454:460	the combined effects	441:460	the combined effects of cold stress and environmental contaminants	441:506	It is therefore important to evaluate the combined effects of cold stress and environmental contaminants.
32738624	1	29	theme	terrestrial	273:283	arg1	invertebrates					285:297	terrestrial invertebrates	273:297	terrestrial invertebrates	273:297	Freezing temperatures is an important stressor in the arctic regions and has a significant influence on the population dynamics and geographic distribution of terrestrial invertebrates.
32738624	9	30	theme	unsaturated	1619:1629	arg1	PLFAs					1631:1635	unsaturated PLFAs	1619:1635	unsaturated PLFAs	1619:1635	Hg had clear negative effects on the proportion of unsaturated PLFAs, which could be an indication of lipid peroxidation.
32738624	9	30	theme	unsaturated	1619:1629	arg1	indication					1656:1665	an indication	1653:1665	an indication of lipid peroxidation	1653:1687	Hg had clear negative effects on the proportion of unsaturated PLFAs, which could be an indication of lipid peroxidation.
32738624	7	31	from	value	1118:1122	arg1	soil					1134:1137	frozen soil	1127:1137	frozen soil	1127:1137	Thus, the LC50 value in frozen soil decreased from 8.3 mg Hg kg-1 (when exposed for 17 days) to only 4.2 mg Hg kg-1 after 36 days' exposure indicating that combined effects of Hg and freezing became larger at prolonged exposure times.
32738624	6	32	theme	contaminated	1025:1036	arg1	soil					1038:1041	Hg contaminated soil	1022:1041	Hg contaminated soil	1022:1041	Freezing at -6 °C had no effect on survival in uncontaminated soil, however, survival of freezing in Hg contaminated soil was clearly reduced, especially at extended exposure times.
32738624	9	33	theme	lipid	1670:1674	arg1	peroxidation					1676:1687	lipid peroxidation	1670:1687	lipid peroxidation	1670:1687	Hg had clear negative effects on the proportion of unsaturated PLFAs, which could be an indication of lipid peroxidation.
32738624	7	34	dep	kg-1	1164:1167	arg1	kg-1					1214:1217	only 4.2 mg Hg kg-1	1199:1217	8.3 mg Hg kg-1 (when exposed for 17 days) to only 4.2 mg Hg kg-1 after 36 days' exposure	1154:1241	Thus, the LC50 value in frozen soil decreased from 8.3 mg Hg kg-1 (when exposed for 17 days) to only 4.2 mg Hg kg-1 after 36 days' exposure indicating that combined effects of Hg and freezing became larger at prolonged exposure times.
32738624	7	34	dep	kg-1	1164:1167	arg1	to					1196:1197	to	1196:1197	to	1196:1197	Thus, the LC50 value in frozen soil decreased from 8.3 mg Hg kg-1 (when exposed for 17 days) to only 4.2 mg Hg kg-1 after 36 days' exposure indicating that combined effects of Hg and freezing became larger at prolonged exposure times.
32738624	7	34	dep	kg-1	1164:1167	arg1	exposed					1175:1181	exposed	1175:1181	exposed for 17 days	1175:1193	Thus, the LC50 value in frozen soil decreased from 8.3 mg Hg kg-1 (when exposed for 17 days) to only 4.2 mg Hg kg-1 after 36 days' exposure indicating that combined effects of Hg and freezing became larger at prolonged exposure times.
32738624	3	35	theme	environmental	481:493	arg1	contaminants					495:506	environmental contaminants	481:506	environmental contaminants	481:506	It is therefore important to evaluate the combined effects of cold stress and environmental contaminants.
32738624	10	36	from	freeze-tolerance	1859:1874	arg1	albidus					1882:1888	E. albidus	1879:1888	E. albidus	1879:1888	Since a high proportion of unsaturated fatty acids in the membrane is important for invertebrate freeze-tolerance, our results suggest that the negative effect of Hg on freeze-tolerance in E. albidus is related to degraded membrane functionality at low temperature.
32738624	10	37	theme	unsaturated	1717:1727	arg1	acids					1735:1739	unsaturated fatty acids	1717:1739	unsaturated fatty acids	1717:1739	Since a high proportion of unsaturated fatty acids in the membrane is important for invertebrate freeze-tolerance, our results suggest that the negative effect of Hg on freeze-tolerance in E. albidus is related to degraded membrane functionality at low temperature.
32738624	6	38	contain	had	939:941	arg1	-6 °C					933:937	Freezing at -6 °C	921:937	Freezing at -6 °C	921:937	Freezing at -6 °C had no effect on survival in uncontaminated soil, however, survival of freezing in Hg contaminated soil was clearly reduced, especially at extended exposure times.
32738624	6	38	contain	had	939:941	arg2	effect					946:951	no effect	943:951	no effect	943:951	Freezing at -6 °C had no effect on survival in uncontaminated soil, however, survival of freezing in Hg contaminated soil was clearly reduced, especially at extended exposure times.
32738624	3	39	theme	stress	470:475	arg1	effects					454:460	the combined effects	441:460	the combined effects of cold stress and environmental contaminants	441:506	It is therefore important to evaluate the combined effects of cold stress and environmental contaminants.
32738624	7	40	theme	LC50	1113:1116	arg1	value					1118:1122	the LC50 value	1109:1122	the LC50 value in frozen soil	1109:1137	Thus, the LC50 value in frozen soil decreased from 8.3 mg Hg kg-1 (when exposed for 17 days) to only 4.2 mg Hg kg-1 after 36 days' exposure indicating that combined effects of Hg and freezing became larger at prolonged exposure times.
32738624	8	41	dep	reserves	1372:1379	arg1	%					1391:1391	almost 50%	1382:1391	almost 50%	1382:1391	Hg caused a depletion of glycogen reserves (almost 50% at 12 mg kg-1 dry soil), but despite this effect worms were able to maintain a constant cryoprotectant level (about 0.12 mg glucose mg-1 dry weight) at all Hg concentrations.
32738624	5	42	theme	for	790:792	arg1	metabolism					794:803	cryoprotection and fuel for metabolism	766:803	metabolism	794:803	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	4	43	from	earthworm	625:633	arg1	aspects					590:596	various physiological aspects	568:596	various physiological aspects of freeze-tolerance in the earthworm (Enchytraeus albidus)	568:655	Here, we aimed to investigate the effects of Hg (HgCl2) on various physiological aspects of freeze-tolerance in the earthworm (Enchytraeus albidus).
32738624	2	44	theme	protective	351:360	arg1	responses					379:387	protective cold-acclimation responses	351:387	protective cold-acclimation responses of organisms	351:400	Toxic metals in the environment can interfere with protective cold-acclimation responses of organisms.
32738624	4	45	from	effects	543:549	arg1	aspects					590:596	various physiological aspects	568:596	various physiological aspects of freeze-tolerance in the earthworm (Enchytraeus albidus)	568:655	Here, we aimed to investigate the effects of Hg (HgCl2) on various physiological aspects of freeze-tolerance in the earthworm (Enchytraeus albidus).
32738624	7	46	theme	Hg	1211:1212	arg1	kg-1					1214:1217	only 4.2 mg Hg kg-1	1199:1217	8.3 mg Hg kg-1 (when exposed for 17 days) to only 4.2 mg Hg kg-1 after 36 days' exposure	1154:1241	Thus, the LC50 value in frozen soil decreased from 8.3 mg Hg kg-1 (when exposed for 17 days) to only 4.2 mg Hg kg-1 after 36 days' exposure indicating that combined effects of Hg and freezing became larger at prolonged exposure times.
32738624	10	47	theme	low	1939:1941	arg1	temperature					1943:1953	low temperature	1939:1953	low temperature	1939:1953	Since a high proportion of unsaturated fatty acids in the membrane is important for invertebrate freeze-tolerance, our results suggest that the negative effect of Hg on freeze-tolerance in E. albidus is related to degraded membrane functionality at low temperature.
32738624	4	48	theme	Hg	554:555	arg1	effects					543:549	the effects	539:549	the effects of Hg (HgCl2) on various physiological aspects of freeze-tolerance in the earthworm (Enchytraeus albidus)	539:655	Here, we aimed to investigate the effects of Hg (HgCl2) on various physiological aspects of freeze-tolerance in the earthworm (Enchytraeus albidus).
32738624	6	49	from	-6 °C	933:937	arg1	at					930:931	Freezing at -6 °C	921:937	Freezing at -6 °C	921:937	Freezing at -6 °C had no effect on survival in uncontaminated soil, however, survival of freezing in Hg contaminated soil was clearly reduced, especially at extended exposure times.
32738624	9	50	contain	had	1571:1573	arg1	Hg					1568:1569	Hg	1568:1569	Hg	1568:1569	Hg had clear negative effects on the proportion of unsaturated PLFAs, which could be an indication of lipid peroxidation.
32738624	9	50	contain	had	1571:1573	arg2	effects					1590:1596	clear negative effects	1575:1596	clear negative effects	1575:1596	Hg had clear negative effects on the proportion of unsaturated PLFAs, which could be an indication of lipid peroxidation.
32738624	8	51	theme	dry	1530:1532	arg1	level					1496:1500	a constant cryoprotectant level	1470:1500	a constant cryoprotectant level (about 0.12 mg glucose mg-1 dry weight)	1470:1540	Hg caused a depletion of glycogen reserves (almost 50% at 12 mg kg-1 dry soil), but despite this effect worms were able to maintain a constant cryoprotectant level (about 0.12 mg glucose mg-1 dry weight) at all Hg concentrations.
32738624	8	51	theme	dry	1530:1532	arg1	weight					1534:1539	about 0.12 mg glucose mg-1 dry weight	1503:1539	about 0.12 mg glucose mg-1 dry weight	1503:1539	Hg caused a depletion of glycogen reserves (almost 50% at 12 mg kg-1 dry soil), but despite this effect worms were able to maintain a constant cryoprotectant level (about 0.12 mg glucose mg-1 dry weight) at all Hg concentrations.
32738624	6	52	theme	extended	1078:1085	arg1	times					1096:1100	extended exposure times	1078:1100	extended exposure times	1078:1100	Freezing at -6 °C had no effect on survival in uncontaminated soil, however, survival of freezing in Hg contaminated soil was clearly reduced, especially at extended exposure times.
32738624	8	53	theme	constant	1472:1479	arg1	level					1496:1500	a constant cryoprotectant level	1470:1500	a constant cryoprotectant level (about 0.12 mg glucose mg-1 dry weight)	1470:1540	Hg caused a depletion of glycogen reserves (almost 50% at 12 mg kg-1 dry soil), but despite this effect worms were able to maintain a constant cryoprotectant level (about 0.12 mg glucose mg-1 dry weight) at all Hg concentrations.
32738624	8	53	theme	constant	1472:1479	arg1	weight					1534:1539	about 0.12 mg glucose mg-1 dry weight	1503:1539	about 0.12 mg glucose mg-1 dry weight	1503:1539	Hg caused a depletion of glycogen reserves (almost 50% at 12 mg kg-1 dry soil), but despite this effect worms were able to maintain a constant cryoprotectant level (about 0.12 mg glucose mg-1 dry weight) at all Hg concentrations.
32738624	1	54	contain	has	187:189	arg1	temperatures					123:134	Freezing temperatures	114:134	Freezing temperatures	114:134	Freezing temperatures is an important stressor in the arctic regions and has a significant influence on the population dynamics and geographic distribution of terrestrial invertebrates.
32738624	1	54	contain	has	187:189	arg2	influence					205:213	a significant influence	191:213	a significant influence on the population dynamics and geographic distribution of terrestrial invertebrates	191:297	Freezing temperatures is an important stressor in the arctic regions and has a significant influence on the population dynamics and geographic distribution of terrestrial invertebrates.
32738624	1	54	contain	has	187:189	arg1	stressor					152:159	an important stressor	139:159	an important stressor in the arctic regions	139:181	Freezing temperatures is an important stressor in the arctic regions and has a significant influence on the population dynamics and geographic distribution of terrestrial invertebrates.
32738624	2	55	theme	organisms	392:400	arg1	responses					379:387	protective cold-acclimation responses	351:387	protective cold-acclimation responses of organisms	351:400	Toxic metals in the environment can interfere with protective cold-acclimation responses of organisms.
32738624	5	56	theme	fatty	863:867	arg1	PLFA					876:879	PLFA	876:879	PLFA	876:879	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	5	56	theme	fatty	863:867	arg1	acids					869:873	membrane phospholipid fatty acids	841:873	membrane phospholipid fatty acids (PLFA)	841:880	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	1	57	theme	population	222:231	arg1	dynamics					233:240	population dynamics	222:240	population dynamics	222:240	Freezing temperatures is an important stressor in the arctic regions and has a significant influence on the population dynamics and geographic distribution of terrestrial invertebrates.
32738624	10	58	from	proportion	1703:1712	arg1	membrane					1748:1755	the membrane	1744:1755	the membrane	1744:1755	Since a high proportion of unsaturated fatty acids in the membrane is important for invertebrate freeze-tolerance, our results suggest that the negative effect of Hg on freeze-tolerance in E. albidus is related to degraded membrane functionality at low temperature.
32738624	4	59	theme	physiological	576:588	arg1	aspects					590:596	various physiological aspects	568:596	various physiological aspects of freeze-tolerance in the earthworm (Enchytraeus albidus)	568:655	Here, we aimed to investigate the effects of Hg (HgCl2) on various physiological aspects of freeze-tolerance in the earthworm (Enchytraeus albidus).
32738624	2	60	theme	Toxic	300:304	arg1	metals					306:311	Toxic metals	300:311	Toxic metals in the environment	300:330	Toxic metals in the environment can interfere with protective cold-acclimation responses of organisms.
32738624	8	61	theme	glucose	1517:1523	arg1	level					1496:1500	a constant cryoprotectant level	1470:1500	a constant cryoprotectant level (about 0.12 mg glucose mg-1 dry weight)	1470:1540	Hg caused a depletion of glycogen reserves (almost 50% at 12 mg kg-1 dry soil), but despite this effect worms were able to maintain a constant cryoprotectant level (about 0.12 mg glucose mg-1 dry weight) at all Hg concentrations.
32738624	8	61	theme	glucose	1517:1523	arg1	weight					1534:1539	about 0.12 mg glucose mg-1 dry weight	1503:1539	about 0.12 mg glucose mg-1 dry weight	1503:1539	Hg caused a depletion of glycogen reserves (almost 50% at 12 mg kg-1 dry soil), but despite this effect worms were able to maintain a constant cryoprotectant level (about 0.12 mg glucose mg-1 dry weight) at all Hg concentrations.
32738624	5	62	theme	phospholipid	850:861	arg1	PLFA					876:879	PLFA	876:879	PLFA	876:879	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	5	62	theme	phospholipid	850:861	arg1	acids					869:873	membrane phospholipid fatty acids	841:873	membrane phospholipid fatty acids (PLFA)	841:880	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	0	63	theme	physiological	31:43	arg1	adaptations					45:55	physiological adaptations	31:55	physiological adaptations to freezing in the arctic earthworm Enchytraeus albidus	31:111	Mercury (Hg2+) interferes with physiological adaptations to freezing in the arctic earthworm Enchytraeus albidus.
32738624	5	64	theme	acids	869:873	arg1	composition					826:836	the composition	822:836	the composition of membrane phospholipid fatty acids (PLFA)	822:880	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	7	65	theme	prolonged	1312:1320	arg1	times					1331:1335	prolonged exposure times	1312:1335	prolonged exposure times	1312:1335	Thus, the LC50 value in frozen soil decreased from 8.3 mg Hg kg-1 (when exposed for 17 days) to only 4.2 mg Hg kg-1 after 36 days' exposure indicating that combined effects of Hg and freezing became larger at prolonged exposure times.
32738624	4	66	theme	Enchytraeus	636:646	arg1	earthworm					625:633	earthworm	625:633	the earthworm (Enchytraeus albidus)	621:655	Here, we aimed to investigate the effects of Hg (HgCl2) on various physiological aspects of freeze-tolerance in the earthworm (Enchytraeus albidus).
32738624	4	66	theme	Enchytraeus	636:646	arg1	albidus					648:654	Enchytraeus albidus	636:654	Enchytraeus albidus	636:654	Here, we aimed to investigate the effects of Hg (HgCl2) on various physiological aspects of freeze-tolerance in the earthworm (Enchytraeus albidus).
32738624	5	67	theme	glycogen	716:723	arg1	levels					674:679	the levels	670:679	the levels of the cryoprotectant glucose	670:709	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	5	67	theme	glycogen	716:723	arg1	changes					811:817	changes	811:817	changes in the composition of membrane phospholipid fatty acids (PLFA)	811:880	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	5	67	theme	glycogen	716:723	arg1	indicator					888:896	an indicator	885:896	an indicator of lipid peroxidation	885:918	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	5	67	theme	glycogen	716:723	arg1	content					725:731	the glycogen content	712:731	the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism)	712:804	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	1	68	theme	significant	193:203	arg1	influence					205:213	a significant influence	191:213	a significant influence on the population dynamics and geographic distribution of terrestrial invertebrates	191:297	Freezing temperatures is an important stressor in the arctic regions and has a significant influence on the population dynamics and geographic distribution of terrestrial invertebrates.
32738624	9	69	theme	negative	1581:1588	arg1	effects					1590:1596	clear negative effects	1575:1596	clear negative effects	1575:1596	Hg had clear negative effects on the proportion of unsaturated PLFAs, which could be an indication of lipid peroxidation.
32738624	8	70	theme	reserves	1372:1379	arg1	depletion					1350:1358	a depletion	1348:1358	a depletion of glycogen reserves (almost 50% at 12 mg kg-1 dry soil)	1348:1415	Hg caused a depletion of glycogen reserves (almost 50% at 12 mg kg-1 dry soil), but despite this effect worms were able to maintain a constant cryoprotectant level (about 0.12 mg glucose mg-1 dry weight) at all Hg concentrations.
32738624	7	71	theme	8.3 mg	1154:1159	arg1	kg-1					1164:1167	8.3 mg Hg kg-1	1154:1167	8.3 mg Hg kg-1 (when exposed for 17 days) to only 4.2 mg Hg kg-1 after 36 days' exposure	1154:1241	Thus, the LC50 value in frozen soil decreased from 8.3 mg Hg kg-1 (when exposed for 17 days) to only 4.2 mg Hg kg-1 after 36 days' exposure indicating that combined effects of Hg and freezing became larger at prolonged exposure times.
32738624	1	72	from	influence	205:213	arg1	dynamics					233:240	population dynamics	222:240	population dynamics	222:240	Freezing temperatures is an important stressor in the arctic regions and has a significant influence on the population dynamics and geographic distribution of terrestrial invertebrates.
32738624	1	72	from	influence	205:213	arg1	distribution					257:268	geographic distribution	246:268	geographic distribution	246:268	Freezing temperatures is an important stressor in the arctic regions and has a significant influence on the population dynamics and geographic distribution of terrestrial invertebrates.
32738624	6	73	theme	Freezing	921:928	arg1	-6 °C					933:937	Freezing at -6 °C	921:937	Freezing at -6 °C	921:937	Freezing at -6 °C had no effect on survival in uncontaminated soil, however, survival of freezing in Hg contaminated soil was clearly reduced, especially at extended exposure times.
32738624	7	74	theme	freezing	1286:1293	arg1	effects					1268:1274	combined effects	1259:1274	combined effects of Hg and freezing	1259:1293	Thus, the LC50 value in frozen soil decreased from 8.3 mg Hg kg-1 (when exposed for 17 days) to only 4.2 mg Hg kg-1 after 36 days' exposure indicating that combined effects of Hg and freezing became larger at prolonged exposure times.
32738624	5	75	theme	cryoprotectant	688:701	arg1	glucose					703:709	the cryoprotectant glucose	684:709	the cryoprotectant glucose	684:709	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	10	76	theme	high	1698:1701	arg1	proportion					1703:1712	a high proportion	1696:1712	a high proportion of unsaturated fatty acids in the membrane	1696:1755	Since a high proportion of unsaturated fatty acids in the membrane is important for invertebrate freeze-tolerance, our results suggest that the negative effect of Hg on freeze-tolerance in E. albidus is related to degraded membrane functionality at low temperature.
32738624	10	76	theme	high	1698:1701	arg1	important					1760:1768	important	1760:1768	important	1760:1768	Since a high proportion of unsaturated fatty acids in the membrane is important for invertebrate freeze-tolerance, our results suggest that the negative effect of Hg on freeze-tolerance in E. albidus is related to degraded membrane functionality at low temperature.
32738624	5	77	theme	lipid	901:905	arg1	peroxidation					907:918	lipid peroxidation	901:918	lipid peroxidation	901:918	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	7	78	theme	Hg	1279:1280	arg1	effects					1268:1274	combined effects	1259:1274	combined effects of Hg and freezing	1259:1293	Thus, the LC50 value in frozen soil decreased from 8.3 mg Hg kg-1 (when exposed for 17 days) to only 4.2 mg Hg kg-1 after 36 days' exposure indicating that combined effects of Hg and freezing became larger at prolonged exposure times.
32738624	10	79	theme	E.	1879:1880	arg1	albidus					1882:1888	E. albidus	1879:1888	E. albidus	1879:1888	Since a high proportion of unsaturated fatty acids in the membrane is important for invertebrate freeze-tolerance, our results suggest that the negative effect of Hg on freeze-tolerance in E. albidus is related to degraded membrane functionality at low temperature.
32738624	5	80	theme	molecules	752:760	arg1	source					734:739	source	734:739	source of glucose molecules for cryoprotection and fuel for metabolism	734:803	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	5	81	dep	content	725:731	arg1	source					734:739	source	734:739	source of glucose molecules for cryoprotection and fuel for metabolism	734:803	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	8	82	theme	12 mg kg-1	1396:1405	arg1	soil					1411:1414	12 mg kg-1 dry soil	1396:1414	12 mg kg-1 dry soil	1396:1414	Hg caused a depletion of glycogen reserves (almost 50% at 12 mg kg-1 dry soil), but despite this effect worms were able to maintain a constant cryoprotectant level (about 0.12 mg glucose mg-1 dry weight) at all Hg concentrations.
32738624	7	83	theme	Hg	1161:1162	arg1	kg-1					1164:1167	8.3 mg Hg kg-1	1154:1167	8.3 mg Hg kg-1 (when exposed for 17 days) to only 4.2 mg Hg kg-1 after 36 days' exposure	1154:1241	Thus, the LC50 value in frozen soil decreased from 8.3 mg Hg kg-1 (when exposed for 17 days) to only 4.2 mg Hg kg-1 after 36 days' exposure indicating that combined effects of Hg and freezing became larger at prolonged exposure times.
32738624	5	84	theme	cryoprotection	766:779	arg1	metabolism					794:803	cryoprotection and fuel for metabolism	766:803	metabolism	794:803	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	2	85	from	metals	306:311	arg1	environment					320:330	the environment	316:330	the environment	316:330	Toxic metals in the environment can interfere with protective cold-acclimation responses of organisms.
32738624	0	86	theme	earthworm	83:91	arg1	albidus					105:111	the arctic earthworm Enchytraeus albidus	72:111	the arctic earthworm Enchytraeus albidus	72:111	Mercury (Hg2+) interferes with physiological adaptations to freezing in the arctic earthworm Enchytraeus albidus.
32738624	0	87	from	adaptations	45:55	arg1	albidus					105:111	the arctic earthworm Enchytraeus albidus	72:111	the arctic earthworm Enchytraeus albidus	72:111	Mercury (Hg2+) interferes with physiological adaptations to freezing in the arctic earthworm Enchytraeus albidus.
32738624	4	88	from	aspects	590:596	arg1	earthworm					625:633	earthworm	625:633	the earthworm (Enchytraeus albidus)	621:655	Here, we aimed to investigate the effects of Hg (HgCl2) on various physiological aspects of freeze-tolerance in the earthworm (Enchytraeus albidus).
32738624	4	88	from	aspects	590:596	arg1	albidus					648:654	Enchytraeus albidus	636:654	Enchytraeus albidus	636:654	Here, we aimed to investigate the effects of Hg (HgCl2) on various physiological aspects of freeze-tolerance in the earthworm (Enchytraeus albidus).
32738624	6	89	from	survival	956:963	arg1	soil					983:986	uncontaminated soil	968:986	uncontaminated soil	968:986	Freezing at -6 °C had no effect on survival in uncontaminated soil, however, survival of freezing in Hg contaminated soil was clearly reduced, especially at extended exposure times.
32738624	3	90	theme	cold	465:468	arg1	stress					470:475	cold stress	465:475	cold stress	465:475	It is therefore important to evaluate the combined effects of cold stress and environmental contaminants.
32738624	7	91	theme	frozen	1127:1132	arg1	soil					1134:1137	frozen soil	1127:1137	frozen soil	1127:1137	Thus, the LC50 value in frozen soil decreased from 8.3 mg Hg kg-1 (when exposed for 17 days) to only 4.2 mg Hg kg-1 after 36 days' exposure indicating that combined effects of Hg and freezing became larger at prolonged exposure times.
32738624	10	92	theme	acids	1735:1739	arg1	proportion					1703:1712	a high proportion	1696:1712	a high proportion of unsaturated fatty acids in the membrane	1696:1755	Since a high proportion of unsaturated fatty acids in the membrane is important for invertebrate freeze-tolerance, our results suggest that the negative effect of Hg on freeze-tolerance in E. albidus is related to degraded membrane functionality at low temperature.
32738624	10	92	theme	acids	1735:1739	arg1	important					1760:1768	important	1760:1768	important	1760:1768	Since a high proportion of unsaturated fatty acids in the membrane is important for invertebrate freeze-tolerance, our results suggest that the negative effect of Hg on freeze-tolerance in E. albidus is related to degraded membrane functionality at low temperature.
32738624	10	93	from	temperature	1943:1953	arg1	related					1893:1899	related	1893:1899	related	1893:1899	Since a high proportion of unsaturated fatty acids in the membrane is important for invertebrate freeze-tolerance, our results suggest that the negative effect of Hg on freeze-tolerance in E. albidus is related to degraded membrane functionality at low temperature.
32738624	10	93	from	temperature	1943:1953	arg1	effect					1843:1848	the negative effect	1830:1848	the negative effect of Hg on freeze-tolerance in E. albidus	1830:1888	Since a high proportion of unsaturated fatty acids in the membrane is important for invertebrate freeze-tolerance, our results suggest that the negative effect of Hg on freeze-tolerance in E. albidus is related to degraded membrane functionality at low temperature.
32738624	4	94	from	freeze-tolerance	601:616	arg1	earthworm					625:633	earthworm	625:633	the earthworm (Enchytraeus albidus)	621:655	Here, we aimed to investigate the effects of Hg (HgCl2) on various physiological aspects of freeze-tolerance in the earthworm (Enchytraeus albidus).
32738624	4	94	from	freeze-tolerance	601:616	arg1	albidus					648:654	Enchytraeus albidus	636:654	Enchytraeus albidus	636:654	Here, we aimed to investigate the effects of Hg (HgCl2) on various physiological aspects of freeze-tolerance in the earthworm (Enchytraeus albidus).
32738624	1	95	theme	invertebrates	285:297	arg1	dynamics					233:240	population dynamics	222:240	population dynamics	222:240	Freezing temperatures is an important stressor in the arctic regions and has a significant influence on the population dynamics and geographic distribution of terrestrial invertebrates.
32738624	1	95	theme	invertebrates	285:297	arg1	distribution					257:268	geographic distribution	246:268	geographic distribution	246:268	Freezing temperatures is an important stressor in the arctic regions and has a significant influence on the population dynamics and geographic distribution of terrestrial invertebrates.
32738624	6	96	theme	Hg	1022:1023	arg1	soil					1038:1041	Hg contaminated soil	1022:1041	Hg contaminated soil	1022:1041	Freezing at -6 °C had no effect on survival in uncontaminated soil, however, survival of freezing in Hg contaminated soil was clearly reduced, especially at extended exposure times.
32738624	9	97	theme	peroxidation	1676:1687	arg1	PLFAs					1631:1635	unsaturated PLFAs	1619:1635	unsaturated PLFAs	1619:1635	Hg had clear negative effects on the proportion of unsaturated PLFAs, which could be an indication of lipid peroxidation.
32738624	9	97	theme	peroxidation	1676:1687	arg1	indication					1656:1665	an indication	1653:1665	an indication of lipid peroxidation	1653:1687	Hg had clear negative effects on the proportion of unsaturated PLFAs, which could be an indication of lipid peroxidation.
32738624	4	98	theme	various	568:574	arg1	aspects					590:596	various physiological aspects	568:596	various physiological aspects of freeze-tolerance in the earthworm (Enchytraeus albidus)	568:655	Here, we aimed to investigate the effects of Hg (HgCl2) on various physiological aspects of freeze-tolerance in the earthworm (Enchytraeus albidus).
32738624	10	99	theme	fatty	1729:1733	arg1	acids					1735:1739	unsaturated fatty acids	1717:1739	unsaturated fatty acids	1717:1739	Since a high proportion of unsaturated fatty acids in the membrane is important for invertebrate freeze-tolerance, our results suggest that the negative effect of Hg on freeze-tolerance in E. albidus is related to degraded membrane functionality at low temperature.
32738624	3	100	theme	contaminants	495:506	arg1	effects					454:460	the combined effects	441:460	the combined effects of cold stress and environmental contaminants	441:506	It is therefore important to evaluate the combined effects of cold stress and environmental contaminants.
32738624	5	101	theme	fuel	785:788	arg1	metabolism					794:803	cryoprotection and fuel for metabolism	766:803	metabolism	794:803	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	6	102	theme	freezing	1010:1017	arg1	survival					998:1005	survival	998:1005	survival of freezing in Hg contaminated soil	998:1041	Freezing at -6 °C had no effect on survival in uncontaminated soil, however, survival of freezing in Hg contaminated soil was clearly reduced, especially at extended exposure times.
32738624	5	103	theme	glucose	744:750	arg1	molecules					752:760	glucose molecules	744:760	glucose molecules	744:760	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	5	104	from	levels	674:679	arg1	composition					826:836	the composition	822:836	the composition of membrane phospholipid fatty acids (PLFA)	822:880	We measured the levels of the cryoprotectant glucose, the glycogen content (source of glucose molecules for cryoprotection and fuel for metabolism), and changes in the composition of membrane phospholipid fatty acids (PLFA) as an indicator of lipid peroxidation.
32738624	7	105	theme	4.2 mg	1204:1209	arg1	kg-1					1214:1217	only 4.2 mg Hg kg-1	1199:1217	8.3 mg Hg kg-1 (when exposed for 17 days) to only 4.2 mg Hg kg-1 after 36 days' exposure	1154:1241	Thus, the LC50 value in frozen soil decreased from 8.3 mg Hg kg-1 (when exposed for 17 days) to only 4.2 mg Hg kg-1 after 36 days' exposure indicating that combined effects of Hg and freezing became larger at prolonged exposure times.
32738624	6	106	theme	exposure	1087:1094	arg1	times					1096:1100	extended exposure times	1078:1100	extended exposure times	1078:1100	Freezing at -6 °C had no effect on survival in uncontaminated soil, however, survival of freezing in Hg contaminated soil was clearly reduced, especially at extended exposure times.
32738624	8	107	theme	cryoprotectant	1481:1494	arg1	level					1496:1500	a constant cryoprotectant level	1470:1500	a constant cryoprotectant level (about 0.12 mg glucose mg-1 dry weight)	1470:1540	Hg caused a depletion of glycogen reserves (almost 50% at 12 mg kg-1 dry soil), but despite this effect worms were able to maintain a constant cryoprotectant level (about 0.12 mg glucose mg-1 dry weight) at all Hg concentrations.
32738624	8	107	theme	cryoprotectant	1481:1494	arg1	weight					1534:1539	about 0.12 mg glucose mg-1 dry weight	1503:1539	about 0.12 mg glucose mg-1 dry weight	1503:1539	Hg caused a depletion of glycogen reserves (almost 50% at 12 mg kg-1 dry soil), but despite this effect worms were able to maintain a constant cryoprotectant level (about 0.12 mg glucose mg-1 dry weight) at all Hg concentrations.
32738624	10	108	theme	membrane	1913:1920	arg1	functionality					1922:1934	degraded membrane functionality	1904:1934	degraded membrane functionality	1904:1934	Since a high proportion of unsaturated fatty acids in the membrane is important for invertebrate freeze-tolerance, our results suggest that the negative effect of Hg on freeze-tolerance in E. albidus is related to degraded membrane functionality at low temperature.
32738624	10	109	theme	invertebrate	1774:1785	arg1	freeze-tolerance					1787:1802	invertebrate freeze-tolerance	1774:1802	invertebrate freeze-tolerance	1774:1802	Since a high proportion of unsaturated fatty acids in the membrane is important for invertebrate freeze-tolerance, our results suggest that the negative effect of Hg on freeze-tolerance in E. albidus is related to degraded membrane functionality at low temperature.
32738624	8	110	from	soil	1411:1414	arg1	%					1391:1391	almost 50%	1382:1391	almost 50%	1382:1391	Hg caused a depletion of glycogen reserves (almost 50% at 12 mg kg-1 dry soil), but despite this effect worms were able to maintain a constant cryoprotectant level (about 0.12 mg glucose mg-1 dry weight) at all Hg concentrations.
32738624	8	111	theme	Hg	1549:1550	arg1	concentrations					1552:1565	all Hg concentrations	1545:1565	all Hg concentrations	1545:1565	Hg caused a depletion of glycogen reserves (almost 50% at 12 mg kg-1 dry soil), but despite this effect worms were able to maintain a constant cryoprotectant level (about 0.12 mg glucose mg-1 dry weight) at all Hg concentrations.
32738624	10	112	theme	Hg	1853:1854	arg1	related					1893:1899	related	1893:1899	related	1893:1899	Since a high proportion of unsaturated fatty acids in the membrane is important for invertebrate freeze-tolerance, our results suggest that the negative effect of Hg on freeze-tolerance in E. albidus is related to degraded membrane functionality at low temperature.
32738624	10	112	theme	Hg	1853:1854	arg1	effect					1843:1848	the negative effect	1830:1848	the negative effect of Hg on freeze-tolerance in E. albidus	1830:1888	Since a high proportion of unsaturated fatty acids in the membrane is important for invertebrate freeze-tolerance, our results suggest that the negative effect of Hg on freeze-tolerance in E. albidus is related to degraded membrane functionality at low temperature.
32600019	14	0	theme	carbohydrate	1776:1787	arg1	flavonoids					1801:1810	flavonoids	1801:1810	flavonoids	1801:1810	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	0	theme	carbohydrate	1776:1787	arg1	carbohydrate					1776:1787	carbohydrate	1776:1787	carbohydrate	1776:1787	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	0	theme	carbohydrate	1776:1787	arg1	alkaloids					1813:1821	alkaloids	1813:1821	alkaloids	1813:1821	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	0	theme	carbohydrate	1776:1787	arg1	glycosides					1828:1837	glycosides	1828:1837	glycosides	1828:1837	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	0	theme	carbohydrate	1776:1787	arg1	C					1798:1798	vitamin C	1790:1798	vitamin C	1790:1798	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	0	theme	carbohydrate	1776:1787	arg1	amounts					1765:1771	appreciable amounts	1753:1771	appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides	1753:1837	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	12	1	dep	significantly	1588:1600	arg1	<					1605:1605	p < 0.05	1603:1610	p < 0.05	1603:1610	Phytochemicals and anti-nutrients were significantly (p < 0.05) more numerous in the UPDJ than PDJ.
32600019	6	2	theme	small	757:761	arg1	pieces					763:768	small pieces	757:768	small pieces (2 cm) (PDJ)	757:781	The skin of one portion was peeled off and sliced into small pieces (2 cm) (PDJ), while the other portion was sliced (2 cm) with the peel (UPDJ).
32600019	6	2	theme	small	757:761	arg1	cm					773:774	2 cm	771:774	2 cm	771:774	The skin of one portion was peeled off and sliced into small pieces (2 cm) (PDJ), while the other portion was sliced (2 cm) with the peel (UPDJ).
32600019	6	2	theme	small	757:761	arg1	PDJ					778:780	PDJ	778:780	PDJ	778:780	The skin of one portion was peeled off and sliced into small pieces (2 cm) (PDJ), while the other portion was sliced (2 cm) with the peel (UPDJ).
32600019	10	3	theme	fat	1341:1343	arg1	contents					1369:1376	significantly (p < 0.05) higher carbohydrate (15.75%), protein (4.72%), fat (2.65%) and ash (0.47%) contents	1269:1376	significantly (p < 0.05) higher carbohydrate (15.75%), protein (4.72%), fat (2.65%) and ash (0.47%) contents	1269:1376	RESULTS The UPDJ had significantly (p < 0.05) higher carbohydrate (15.75%), protein (4.72%), fat (2.65%) and ash (0.47%) contents than PDJ.
32600019	11	4	theme	significant	1391:1401	arg1	difference					1403:1412	No significant difference	1388:1412	No significant difference	1388:1412	No significant difference existed in both vitamin and mineral contents of the two samples except for zinc, which was significantly (p < 0.05) higher in the PDJ.
32600019	5	5	theme	rural	609:613	arg1	communities					615:625	rural communities	609:625	rural communities in Nsukka, Enugu State, Nigeria	609:657	METHODS Fruits harvested from rural communities in Nsukka, Enugu State, Nigeria were washed and divided into two portions.
32600019	1	6	theme	novel	164:168	arg1	sources					170:176	potential novel sources	154:176	potential novel sources of macro- and micro-nutrients	154:206	BACKGROUND Many underutilized wild fruits offer potential novel sources of macro- and micro-nutrients, as well as health-promoting antioxidants and phytochemicals.
32600019	8	7	theme	hedonic	1055:1061	arg1	scale					1063:1067	A 9-point hedonic scale	1045:1067	A 9-point hedonic scale	1045:1067	A 9-point hedonic scale was used to evaluate the sensory properties of the juices.
32600019	10	8	theme	ash	1357:1359	arg1	contents					1369:1376	significantly (p < 0.05) higher carbohydrate (15.75%), protein (4.72%), fat (2.65%) and ash (0.47%) contents	1269:1376	significantly (p < 0.05) higher carbohydrate (15.75%), protein (4.72%), fat (2.65%) and ash (0.47%) contents	1269:1376	RESULTS The UPDJ had significantly (p < 0.05) higher carbohydrate (15.75%), protein (4.72%), fat (2.65%) and ash (0.47%) contents than PDJ.
32600019	14	9	dep	CONCLUSIONS	1715:1725	arg1	contain					1745:1751	contain	1745:1751	contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides	1745:1837	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	7	10	theme	muslin	943:948	arg1	cloth					950:954	a clean muslin cloth	935:954	a clean muslin cloth	935:954	Each portion was liquefied with a high-speed Kenwood kitchen blender, filtered through a clean muslin cloth and evaluated for proximate, mineral, vitamin, phytochemical and anti-nutrient contents.
32600019	7	11	theme	anti-nutrient	1021:1033	arg1	contents					1035:1042	anti-nutrient contents	1021:1042	anti-nutrient contents	1021:1042	Each portion was liquefied with a high-speed Kenwood kitchen blender, filtered through a clean muslin cloth and evaluated for proximate, mineral, vitamin, phytochemical and anti-nutrient contents.
32600019	7	12	theme	high-speed	882:891	arg1	blender					909:915	a high-speed Kenwood kitchen blender	880:915	a high-speed Kenwood kitchen blender	880:915	Each portion was liquefied with a high-speed Kenwood kitchen blender, filtered through a clean muslin cloth and evaluated for proximate, mineral, vitamin, phytochemical and anti-nutrient contents.
32600019	14	13	contain	contain	1745:1751	arg2	amounts					1765:1771	appreciable amounts	1753:1771	appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides	1753:1837	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	13	contain	contain	1745:1751	arg2	alkaloids					1813:1821	alkaloids	1813:1821	alkaloids	1813:1821	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	13	contain	contain	1745:1751	arg2	glycosides					1828:1837	glycosides	1828:1837	glycosides	1828:1837	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	13	contain	contain	1745:1751	arg2	C					1798:1798	vitamin C	1790:1798	vitamin C	1790:1798	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	13	contain	contain	1745:1751	arg2	flavonoids					1801:1810	flavonoids	1801:1810	flavonoids	1801:1810	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	13	contain	contain	1745:1751	arg1	samples					1737:1743	The juice samples	1727:1743	The juice samples	1727:1743	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	13	contain	contain	1745:1751	arg2	carbohydrate					1776:1787	carbohydrate	1776:1787	carbohydrate	1776:1787	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	14	theme	alkaloids	1813:1821	arg1	flavonoids					1801:1810	flavonoids	1801:1810	flavonoids	1801:1810	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	14	theme	alkaloids	1813:1821	arg1	carbohydrate					1776:1787	carbohydrate	1776:1787	carbohydrate	1776:1787	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	14	theme	alkaloids	1813:1821	arg1	alkaloids					1813:1821	alkaloids	1813:1821	alkaloids	1813:1821	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	14	theme	alkaloids	1813:1821	arg1	glycosides					1828:1837	glycosides	1828:1837	glycosides	1828:1837	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	14	theme	alkaloids	1813:1821	arg1	C					1798:1798	vitamin C	1790:1798	vitamin C	1790:1798	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	14	theme	alkaloids	1813:1821	arg1	amounts					1765:1771	appreciable amounts	1753:1771	appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides	1753:1837	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	8	15	used	used	1073:1076	arg2	scale					1063:1067	A 9-point hedonic scale	1045:1067	A 9-point hedonic scale	1045:1067	A 9-point hedonic scale was used to evaluate the sensory properties of the juices.
32600019	1	16	theme	Many	117:120	arg1	fruits					141:146	BACKGROUND Many underutilized wild fruits	106:146	BACKGROUND Many underutilized wild fruits	106:146	BACKGROUND Many underutilized wild fruits offer potential novel sources of macro- and micro-nutrients, as well as health-promoting antioxidants and phytochemicals.
32600019	11	17	theme	samples	1470:1476	arg1	vitamin					1430:1436	both vitamin and mineral contents	1425:1457	vitamin	1430:1436	No significant difference existed in both vitamin and mineral contents of the two samples except for zinc, which was significantly (p < 0.05) higher in the PDJ.
32600019	11	17	theme	samples	1470:1476	arg1	contents					1450:1457	both vitamin and mineral contents	1425:1457	contents	1450:1457	No significant difference existed in both vitamin and mineral contents of the two samples except for zinc, which was significantly (p < 0.05) higher in the PDJ.
32600019	1	18	theme	wild	136:139	arg1	fruits					141:146	BACKGROUND Many underutilized wild fruits	106:146	BACKGROUND Many underutilized wild fruits	106:146	BACKGROUND Many underutilized wild fruits offer potential novel sources of macro- and micro-nutrients, as well as health-promoting antioxidants and phytochemicals.
32600019	16	19	theme	Dika	1890:1893	arg1	fruit					1895:1899	Dika fruit	1890:1899	Dika fruit	1890:1899	Dika fruit is locally available and could be incorporated into meals to promote good health.
32600019	10	20	theme	p	1284:1284	arg1	<					1286:1286	p < 0.05	1284:1291	p < 0.05	1284:1291	RESULTS The UPDJ had significantly (p < 0.05) higher carbohydrate (15.75%), protein (4.72%), fat (2.65%) and ash (0.47%) contents than PDJ.
32600019	0	21	theme	dika	66:69	arg1	juice					99:103	peeled and unpeeled dika (Irvingia gabonensis) fruit juice	46:103	peeled and unpeeled dika (Irvingia gabonensis) fruit juice	46:103	Nutrient, non-nutrient and sensory profile of peeled and unpeeled dika (Irvingia gabonensis) fruit juice.
32600019	15	22	theme	acceptable	1871:1880	arg1	levels					1882:1887	acceptable levels	1871:1887	acceptable levels	1871:1887	The anti-nutrients were within acceptable levels.
32600019	0	23	theme	Irvingia	72:79	arg1	juice					99:103	peeled and unpeeled dika (Irvingia gabonensis) fruit juice	46:103	peeled and unpeeled dika (Irvingia gabonensis) fruit juice	46:103	Nutrient, non-nutrient and sensory profile of peeled and unpeeled dika (Irvingia gabonensis) fruit juice.
32600019	9	24	theme	Statistical	1159:1169	arg1	version					1199:1205	version 21	1199:1208	version 21	1199:1208	The data were analyzed using a Statistical Package for Social Science, version 21 and significance was set at p ≤ 0.05.
32600019	9	24	theme	Statistical	1159:1169	arg1	Package					1171:1177	a Statistical Package	1157:1177	a Statistical Package for Social Science	1157:1196	The data were analyzed using a Statistical Package for Social Science, version 21 and significance was set at p ≤ 0.05.
32600019	10	25	theme	higher	1294:1299	arg1	contents					1369:1376	significantly (p < 0.05) higher carbohydrate (15.75%), protein (4.72%), fat (2.65%) and ash (0.47%) contents	1269:1376	significantly (p < 0.05) higher carbohydrate (15.75%), protein (4.72%), fat (2.65%) and ash (0.47%) contents	1269:1376	RESULTS The UPDJ had significantly (p < 0.05) higher carbohydrate (15.75%), protein (4.72%), fat (2.65%) and ash (0.47%) contents than PDJ.
32600019	12	26	theme	p	1603:1603	arg1	<					1605:1605	p < 0.05	1603:1610	p < 0.05	1603:1610	Phytochemicals and anti-nutrients were significantly (p < 0.05) more numerous in the UPDJ than PDJ.
32600019	5	27	dep	Enugu	638:642	arg1	State					644:648	State	644:648	State	644:648	METHODS Fruits harvested from rural communities in Nsukka, Enugu State, Nigeria were washed and divided into two portions.
32600019	7	28	dep	phytochemical	1003:1015	arg1	vitamin					994:1000	mineral, vitamin, phytochemical	985:1015	vitamin	994:1000	Each portion was liquefied with a high-speed Kenwood kitchen blender, filtered through a clean muslin cloth and evaluated for proximate, mineral, vitamin, phytochemical and anti-nutrient contents.
32600019	3	29	theme	fruits	412:417	arg1	fruits					412:417	such underutilized wild fruits	388:417	such underutilized wild fruits	388:417	Dika fruit is one of such underutilized wild fruits.
32600019	3	29	theme	fruits	412:417	arg1	one					381:383	one	381:383	one	381:383	Dika fruit is one of such underutilized wild fruits.
32600019	5	30	from	communities	615:625	arg1	Enugu					638:642	Enugu	638:642	Enugu	638:642	METHODS Fruits harvested from rural communities in Nsukka, Enugu State, Nigeria were washed and divided into two portions.
32600019	5	30	from	communities	615:625	arg1	Nigeria					651:657	Nigeria	651:657	Nigeria	651:657	METHODS Fruits harvested from rural communities in Nsukka, Enugu State, Nigeria were washed and divided into two portions.
32600019	3	31	theme	Dika	367:370	arg1	fruit					372:376	Dika fruit	367:376	Dika fruit	367:376	Dika fruit is one of such underutilized wild fruits.
32600019	3	32	theme	underutilized	393:405	arg1	fruits					412:417	such underutilized wild fruits	388:417	such underutilized wild fruits	388:417	Dika fruit is one of such underutilized wild fruits.
32600019	11	33	theme	mineral	1442:1448	arg1	contents					1450:1457	both vitamin and mineral contents	1425:1457	contents	1450:1457	No significant difference existed in both vitamin and mineral contents of the two samples except for zinc, which was significantly (p < 0.05) higher in the PDJ.
32600019	0	34	theme	sensory	27:33	arg1	profile					35:41	sensory profile	27:41	sensory profile	27:41	Nutrient, non-nutrient and sensory profile of peeled and unpeeled dika (Irvingia gabonensis) fruit juice.
32600019	0	34	theme	sensory	27:33	arg1	Nutrient					0:7	Nutrient	0:7	Nutrient	0:7	Nutrient, non-nutrient and sensory profile of peeled and unpeeled dika (Irvingia gabonensis) fruit juice.
32600019	7	35	dep	filtered	918:925	arg1	liquefied					865:873	liquefied	865:873	liquefied	865:873	Each portion was liquefied with a high-speed Kenwood kitchen blender, filtered through a clean muslin cloth and evaluated for proximate, mineral, vitamin, phytochemical and anti-nutrient contents.
32600019	4	36	theme	nutrient	502:509	arg1	contents					528:535	nutrient and non-nutrient contents	502:535	nutrient and non-nutrient contents	502:535	This study was carried out to analyze juice samples processed from dika fruit for nutrient and non-nutrient contents, as well as for their sensory attributes.
32600019	14	37	theme	juice	1731:1735	arg1	samples					1737:1743	The juice samples	1727:1743	The juice samples	1727:1743	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	6	38	theme	portion	718:724	arg1	skin					706:709	The skin	702:709	The skin of one portion	702:724	The skin of one portion was peeled off and sliced into small pieces (2 cm) (PDJ), while the other portion was sliced (2 cm) with the peel (UPDJ).
32600019	4	39	theme	non-nutrient	515:526	arg1	contents					528:535	nutrient and non-nutrient contents	502:535	nutrient and non-nutrient contents	502:535	This study was carried out to analyze juice samples processed from dika fruit for nutrient and non-nutrient contents, as well as for their sensory attributes.
32600019	10	40	contain	had	1265:1267	arg2	contents					1369:1376	significantly (p < 0.05) higher carbohydrate (15.75%), protein (4.72%), fat (2.65%) and ash (0.47%) contents	1269:1376	significantly (p < 0.05) higher carbohydrate (15.75%), protein (4.72%), fat (2.65%) and ash (0.47%) contents	1269:1376	RESULTS The UPDJ had significantly (p < 0.05) higher carbohydrate (15.75%), protein (4.72%), fat (2.65%) and ash (0.47%) contents than PDJ.
32600019	10	40	contain	had	1265:1267	arg1	UPDJ					1260:1263	The UPDJ	1256:1263	The UPDJ	1256:1263	RESULTS The UPDJ had significantly (p < 0.05) higher carbohydrate (15.75%), protein (4.72%), fat (2.65%) and ash (0.47%) contents than PDJ.
32600019	13	41	from	UPDJ	1683:1686	arg1	attributes					1703:1712	all sensory attributes	1691:1712	all sensory attributes	1691:1712	The PDJ was rated higher than the UPDJ in all sensory attributes.
32600019	7	42	theme	mineral	985:991	arg1	phytochemical					1003:1015	mineral, vitamin, phytochemical	985:1015	phytochemical	1003:1015	Each portion was liquefied with a high-speed Kenwood kitchen blender, filtered through a clean muslin cloth and evaluated for proximate, mineral, vitamin, phytochemical and anti-nutrient contents.
32600019	6	43	dep	sliced	812:817	arg1	cm					822:823	2 cm	820:823	2 cm	820:823	The skin of one portion was peeled off and sliced into small pieces (2 cm) (PDJ), while the other portion was sliced (2 cm) with the peel (UPDJ).
32600019	2	44	from	information	333:343	arg1	composition					354:364	their composition	348:364	their composition	348:364	However, these benefits are not fully harnessed due to limited information on their composition.
32600019	4	45	theme	sensory	559:565	arg1	attributes					567:576	their sensory attributes	553:576	their sensory attributes	553:576	This study was carried out to analyze juice samples processed from dika fruit for nutrient and non-nutrient contents, as well as for their sensory attributes.
32600019	1	46	theme	potential	154:162	arg1	sources					170:176	potential novel sources	154:176	potential novel sources of macro- and micro-nutrients	154:206	BACKGROUND Many underutilized wild fruits offer potential novel sources of macro- and micro-nutrients, as well as health-promoting antioxidants and phytochemicals.
32600019	6	47	theme	other	794:798	arg1	portion					800:806	the other portion	790:806	the other portion	790:806	The skin of one portion was peeled off and sliced into small pieces (2 cm) (PDJ), while the other portion was sliced (2 cm) with the peel (UPDJ).
32600019	8	48	theme	sensory	1094:1100	arg1	properties					1102:1111	the sensory properties	1090:1111	the sensory properties of the juices	1090:1125	A 9-point hedonic scale was used to evaluate the sensory properties of the juices.
32600019	8	49	theme	9-point	1047:1053	arg1	scale					1063:1067	A 9-point hedonic scale	1045:1067	A 9-point hedonic scale	1045:1067	A 9-point hedonic scale was used to evaluate the sensory properties of the juices.
32600019	14	50	theme	flavonoids	1801:1810	arg1	flavonoids					1801:1810	flavonoids	1801:1810	flavonoids	1801:1810	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	50	theme	flavonoids	1801:1810	arg1	carbohydrate					1776:1787	carbohydrate	1776:1787	carbohydrate	1776:1787	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	50	theme	flavonoids	1801:1810	arg1	alkaloids					1813:1821	alkaloids	1813:1821	alkaloids	1813:1821	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	50	theme	flavonoids	1801:1810	arg1	glycosides					1828:1837	glycosides	1828:1837	glycosides	1828:1837	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	50	theme	flavonoids	1801:1810	arg1	C					1798:1798	vitamin C	1790:1798	vitamin C	1790:1798	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	50	theme	flavonoids	1801:1810	arg1	amounts					1765:1771	appreciable amounts	1753:1771	appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides	1753:1837	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	7	51	theme	clean	937:941	arg1	cloth					950:954	a clean muslin cloth	935:954	a clean muslin cloth	935:954	Each portion was liquefied with a high-speed Kenwood kitchen blender, filtered through a clean muslin cloth and evaluated for proximate, mineral, vitamin, phytochemical and anti-nutrient contents.
32600019	1	52	theme	macro-	181:186	arg1	sources					170:176	potential novel sources	154:176	potential novel sources of macro- and micro-nutrients	154:206	BACKGROUND Many underutilized wild fruits offer potential novel sources of macro- and micro-nutrients, as well as health-promoting antioxidants and phytochemicals.
32600019	1	52	theme	macro-	181:186	arg1	phytochemicals					254:267	phytochemicals	254:267	phytochemicals	254:267	BACKGROUND Many underutilized wild fruits offer potential novel sources of macro- and micro-nutrients, as well as health-promoting antioxidants and phytochemicals.
32600019	1	52	theme	macro-	181:186	arg1	antioxidants					237:248	antioxidants	237:248	antioxidants	237:248	BACKGROUND Many underutilized wild fruits offer potential novel sources of macro- and micro-nutrients, as well as health-promoting antioxidants and phytochemicals.
32600019	8	53	theme	juices	1120:1125	arg1	properties					1102:1111	the sensory properties	1090:1111	the sensory properties of the juices	1090:1125	A 9-point hedonic scale was used to evaluate the sensory properties of the juices.
32600019	14	54	theme	glycosides	1828:1837	arg1	flavonoids					1801:1810	flavonoids	1801:1810	flavonoids	1801:1810	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	54	theme	glycosides	1828:1837	arg1	carbohydrate					1776:1787	carbohydrate	1776:1787	carbohydrate	1776:1787	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	54	theme	glycosides	1828:1837	arg1	alkaloids					1813:1821	alkaloids	1813:1821	alkaloids	1813:1821	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	54	theme	glycosides	1828:1837	arg1	glycosides					1828:1837	glycosides	1828:1837	glycosides	1828:1837	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	54	theme	glycosides	1828:1837	arg1	C					1798:1798	vitamin C	1790:1798	vitamin C	1790:1798	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	54	theme	glycosides	1828:1837	arg1	amounts					1765:1771	appreciable amounts	1753:1771	appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides	1753:1837	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	11	55	theme	p	1520:1520	arg1	<					1522:1522	p < 0.05	1520:1527	p < 0.05	1520:1527	No significant difference existed in both vitamin and mineral contents of the two samples except for zinc, which was significantly (p < 0.05) higher in the PDJ.
32600019	1	56	theme	micro-nutrients	192:206	arg1	sources					170:176	potential novel sources	154:176	potential novel sources of macro- and micro-nutrients	154:206	BACKGROUND Many underutilized wild fruits offer potential novel sources of macro- and micro-nutrients, as well as health-promoting antioxidants and phytochemicals.
32600019	1	56	theme	micro-nutrients	192:206	arg1	phytochemicals					254:267	phytochemicals	254:267	phytochemicals	254:267	BACKGROUND Many underutilized wild fruits offer potential novel sources of macro- and micro-nutrients, as well as health-promoting antioxidants and phytochemicals.
32600019	1	56	theme	micro-nutrients	192:206	arg1	antioxidants					237:248	antioxidants	237:248	antioxidants	237:248	BACKGROUND Many underutilized wild fruits offer potential novel sources of macro- and micro-nutrients, as well as health-promoting antioxidants and phytochemicals.
32600019	7	57	theme	Kenwood	893:899	arg1	blender					909:915	a high-speed Kenwood kitchen blender	880:915	a high-speed Kenwood kitchen blender	880:915	Each portion was liquefied with a high-speed Kenwood kitchen blender, filtered through a clean muslin cloth and evaluated for proximate, mineral, vitamin, phytochemical and anti-nutrient contents.
32600019	16	58	theme	good	1970:1973	arg1	health					1975:1980	good health	1970:1980	good health	1970:1980	Dika fruit is locally available and could be incorporated into meals to promote good health.
32600019	7	59	theme	kitchen	901:907	arg1	blender					909:915	a high-speed Kenwood kitchen blender	880:915	a high-speed Kenwood kitchen blender	880:915	Each portion was liquefied with a high-speed Kenwood kitchen blender, filtered through a clean muslin cloth and evaluated for proximate, mineral, vitamin, phytochemical and anti-nutrient contents.
32600019	1	60	theme	BACKGROUND	106:115	arg1	fruits					141:146	BACKGROUND Many underutilized wild fruits	106:146	BACKGROUND Many underutilized wild fruits	106:146	BACKGROUND Many underutilized wild fruits offer potential novel sources of macro- and micro-nutrients, as well as health-promoting antioxidants and phytochemicals.
32600019	0	61	theme	unpeeled	57:64	arg1	juice					99:103	peeled and unpeeled dika (Irvingia gabonensis) fruit juice	46:103	peeled and unpeeled dika (Irvingia gabonensis) fruit juice	46:103	Nutrient, non-nutrient and sensory profile of peeled and unpeeled dika (Irvingia gabonensis) fruit juice.
32600019	1	62	theme	underutilized	122:134	arg1	fruits					141:146	BACKGROUND Many underutilized wild fruits	106:146	BACKGROUND Many underutilized wild fruits	106:146	BACKGROUND Many underutilized wild fruits offer potential novel sources of macro- and micro-nutrients, as well as health-promoting antioxidants and phytochemicals.
32600019	12	63	from	numerous	1618:1625	arg1	PDJ					1644:1646	PDJ	1644:1646	PDJ	1644:1646	Phytochemicals and anti-nutrients were significantly (p < 0.05) more numerous in the UPDJ than PDJ.
32600019	4	64	theme	dika	487:490	arg1	fruit					492:496	dika fruit	487:496	dika fruit	487:496	This study was carried out to analyze juice samples processed from dika fruit for nutrient and non-nutrient contents, as well as for their sensory attributes.
32600019	0	65	theme	juice	99:103	arg1	non-nutrient					10:21	non-nutrient	10:21	non-nutrient	10:21	Nutrient, non-nutrient and sensory profile of peeled and unpeeled dika (Irvingia gabonensis) fruit juice.
32600019	0	65	theme	juice	99:103	arg1	profile					35:41	sensory profile	27:41	sensory profile	27:41	Nutrient, non-nutrient and sensory profile of peeled and unpeeled dika (Irvingia gabonensis) fruit juice.
32600019	0	65	theme	juice	99:103	arg1	Nutrient					0:7	Nutrient	0:7	Nutrient	0:7	Nutrient, non-nutrient and sensory profile of peeled and unpeeled dika (Irvingia gabonensis) fruit juice.
32600019	12	66	dep	PDJ	1644:1646	arg1	UPDJ					1634:1637	UPDJ	1634:1637	UPDJ	1634:1637	Phytochemicals and anti-nutrients were significantly (p < 0.05) more numerous in the UPDJ than PDJ.
32600019	12	66	dep	PDJ	1644:1646	arg1	the					1630:1632	the	1630:1632	the	1630:1632	Phytochemicals and anti-nutrients were significantly (p < 0.05) more numerous in the UPDJ than PDJ.
32600019	0	67	theme	gabonensis	81:90	arg1	juice					99:103	peeled and unpeeled dika (Irvingia gabonensis) fruit juice	46:103	peeled and unpeeled dika (Irvingia gabonensis) fruit juice	46:103	Nutrient, non-nutrient and sensory profile of peeled and unpeeled dika (Irvingia gabonensis) fruit juice.
32600019	5	68	theme	METHODS	579:585	arg1	Fruits					587:592	METHODS Fruits	579:592	METHODS Fruits harvested from rural communities in Nsukka, Enugu State, Nigeria	579:657	METHODS Fruits harvested from rural communities in Nsukka, Enugu State, Nigeria were washed and divided into two portions.
32600019	4	69	theme	juice	458:462	arg1	samples					464:470	juice samples	458:470	juice samples processed from dika fruit for nutrient and non-nutrient contents, as well as for their sensory attributes	458:576	This study was carried out to analyze juice samples processed from dika fruit for nutrient and non-nutrient contents, as well as for their sensory attributes.
32600019	14	70	theme	C	1798:1798	arg1	flavonoids					1801:1810	flavonoids	1801:1810	flavonoids	1801:1810	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	70	theme	C	1798:1798	arg1	carbohydrate					1776:1787	carbohydrate	1776:1787	carbohydrate	1776:1787	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	70	theme	C	1798:1798	arg1	alkaloids					1813:1821	alkaloids	1813:1821	alkaloids	1813:1821	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	70	theme	C	1798:1798	arg1	glycosides					1828:1837	glycosides	1828:1837	glycosides	1828:1837	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	70	theme	C	1798:1798	arg1	C					1798:1798	vitamin C	1790:1798	vitamin C	1790:1798	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	70	theme	C	1798:1798	arg1	amounts					1765:1771	appreciable amounts	1753:1771	appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides	1753:1837	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	0	71	theme	fruit	93:97	arg1	juice					99:103	peeled and unpeeled dika (Irvingia gabonensis) fruit juice	46:103	peeled and unpeeled dika (Irvingia gabonensis) fruit juice	46:103	Nutrient, non-nutrient and sensory profile of peeled and unpeeled dika (Irvingia gabonensis) fruit juice.
32600019	11	72	dep	higher	1530:1535	arg1	<					1522:1522	p < 0.05	1520:1527	p < 0.05	1520:1527	No significant difference existed in both vitamin and mineral contents of the two samples except for zinc, which was significantly (p < 0.05) higher in the PDJ.
32600019	14	73	theme	vitamin	1790:1796	arg1	C					1798:1798	vitamin C	1790:1798	vitamin C	1790:1798	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	10	74	dep	higher	1294:1299	arg1	<					1286:1286	p < 0.05	1284:1291	p < 0.05	1284:1291	RESULTS The UPDJ had significantly (p < 0.05) higher carbohydrate (15.75%), protein (4.72%), fat (2.65%) and ash (0.47%) contents than PDJ.
32600019	14	75	theme	appreciable	1753:1763	arg1	flavonoids					1801:1810	flavonoids	1801:1810	flavonoids	1801:1810	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	75	theme	appreciable	1753:1763	arg1	carbohydrate					1776:1787	carbohydrate	1776:1787	carbohydrate	1776:1787	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	75	theme	appreciable	1753:1763	arg1	alkaloids					1813:1821	alkaloids	1813:1821	alkaloids	1813:1821	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	75	theme	appreciable	1753:1763	arg1	glycosides					1828:1837	glycosides	1828:1837	glycosides	1828:1837	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	75	theme	appreciable	1753:1763	arg1	C					1798:1798	vitamin C	1790:1798	vitamin C	1790:1798	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	14	75	theme	appreciable	1753:1763	arg1	amounts					1765:1771	appreciable amounts	1753:1771	appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides	1753:1837	CONCLUSIONS The juice samples contain appreciable amounts of carbohydrate, vitamin C, flavonoids, alkaloids, and glycosides.
32600019	3	76	theme	wild	407:410	arg1	fruits					412:417	such underutilized wild fruits	388:417	such underutilized wild fruits	388:417	Dika fruit is one of such underutilized wild fruits.
32600019	10	77	theme	carbohydrate	1301:1312	arg1	contents					1369:1376	significantly (p < 0.05) higher carbohydrate (15.75%), protein (4.72%), fat (2.65%) and ash (0.47%) contents	1269:1376	significantly (p < 0.05) higher carbohydrate (15.75%), protein (4.72%), fat (2.65%) and ash (0.47%) contents	1269:1376	RESULTS The UPDJ had significantly (p < 0.05) higher carbohydrate (15.75%), protein (4.72%), fat (2.65%) and ash (0.47%) contents than PDJ.
32600019	0	78	theme	peeled	46:51	arg1	juice					99:103	peeled and unpeeled dika (Irvingia gabonensis) fruit juice	46:103	peeled and unpeeled dika (Irvingia gabonensis) fruit juice	46:103	Nutrient, non-nutrient and sensory profile of peeled and unpeeled dika (Irvingia gabonensis) fruit juice.
32600019	3	79	theme	such	388:391	arg1	fruits					412:417	such underutilized wild fruits	388:417	such underutilized wild fruits	388:417	Dika fruit is one of such underutilized wild fruits.
32600019	10	80	theme	protein	1324:1330	arg1	contents					1369:1376	significantly (p < 0.05) higher carbohydrate (15.75%), protein (4.72%), fat (2.65%) and ash (0.47%) contents	1269:1376	significantly (p < 0.05) higher carbohydrate (15.75%), protein (4.72%), fat (2.65%) and ash (0.47%) contents	1269:1376	RESULTS The UPDJ had significantly (p < 0.05) higher carbohydrate (15.75%), protein (4.72%), fat (2.65%) and ash (0.47%) contents than PDJ.
32600019	13	81	theme	sensory	1695:1701	arg1	attributes					1703:1712	all sensory attributes	1691:1712	all sensory attributes	1691:1712	The PDJ was rated higher than the UPDJ in all sensory attributes.
32600019	2	82	theme	limited	325:331	arg1	information					333:343	limited information	325:343	limited information on their composition	325:364	However, these benefits are not fully harnessed due to limited information on their composition.
32600019	10	83	dep	RESULTS	1248:1254	arg1	had					1265:1267	had	1265:1267	had significantly (p < 0.05) higher carbohydrate (15.75%), protein (4.72%), fat (2.65%) and ash (0.47%) contents than PDJ	1265:1385	RESULTS The UPDJ had significantly (p < 0.05) higher carbohydrate (15.75%), protein (4.72%), fat (2.65%) and ash (0.47%) contents than PDJ.
32600019	12	84	from	PDJ	1644:1646	arg1	numerous					1618:1625	numerous	1618:1625	numerous	1618:1625	Phytochemicals and anti-nutrients were significantly (p < 0.05) more numerous in the UPDJ than PDJ.
32600019	1	85	theme	health-promoting	220:235	arg1	antioxidants					237:248	antioxidants	237:248	antioxidants	237:248	BACKGROUND Many underutilized wild fruits offer potential novel sources of macro- and micro-nutrients, as well as health-promoting antioxidants and phytochemicals.
34593921	0	0	theme	exercise	86:93	arg1	performance					95:105	exercise performance	86:105	exercise performance	86:105	Effectiveness of human-origin Lactobacillus plantarum PL-02 in improving muscle mass, exercise performance and anti-fatigue.
34593921	1	1	theme	energy	162:167	arg1	metabolism					169:178	energy metabolism	162:178	energy metabolism	162:178	Gut microbiota is very important for energy metabolism and regulation, which in turn affect the health and physiological functions of the host, and provide energy required for exercise.
34593921	4	2	theme	gold	665:668	arg1	medalist					670:677	gold medalist	665:677	gold medalist	665:677	In this study, we screened Lactobacillus plantarum (PL-02), a probiotic of human-origin, from the intestines of 2008 Olympic women's 48 kg weightlifting gold medalist and explored the role of PL-02 in improved exercise endurance performance, reduced fatigue biochemical parameters, and changes in body composition.
34593921	6	3	theme	supplementation	1042:1056	arg1	4 weeks					1025:1031	4 weeks	1025:1031	4 weeks of PL-02 supplementation	1025:1056	The results showed that 4 weeks of PL-02 supplementation could significantly increase muscle mass, muscle strength and endurance performance, and hepatic and muscular glycogen storage.
34593921	6	4	theme	glycogen	1168:1175	arg1	storage					1177:1183	hepatic and muscular glycogen storage	1147:1183	hepatic and muscular glycogen storage	1147:1183	The results showed that 4 weeks of PL-02 supplementation could significantly increase muscle mass, muscle strength and endurance performance, and hepatic and muscular glycogen storage.
34593921	4	5	from	performance	741:751	arg1	composition					814:824	body composition	809:824	body composition	809:824	In this study, we screened Lactobacillus plantarum (PL-02), a probiotic of human-origin, from the intestines of 2008 Olympic women's 48 kg weightlifting gold medalist and explored the role of PL-02 in improved exercise endurance performance, reduced fatigue biochemical parameters, and changes in body composition.
34593921	4	6	theme	endurance	731:739	arg1	performance					741:751	improved exercise endurance performance	713:751	improved exercise endurance performance	713:751	In this study, we screened Lactobacillus plantarum (PL-02), a probiotic of human-origin, from the intestines of 2008 Olympic women's 48 kg weightlifting gold medalist and explored the role of PL-02 in improved exercise endurance performance, reduced fatigue biochemical parameters, and changes in body composition.
34593921	4	7	theme	Olympic	629:635	arg1	48 kg					645:649	2008 Olympic women's 48 kg	624:649	2008 Olympic women's 48 kg weightlifting gold medalist	624:677	In this study, we screened Lactobacillus plantarum (PL-02), a probiotic of human-origin, from the intestines of 2008 Olympic women's 48 kg weightlifting gold medalist and explored the role of PL-02 in improved exercise endurance performance, reduced fatigue biochemical parameters, and changes in body composition.
34593921	2	8	with	Supplementation	311:325	arg1	probiotics					332:341	probiotics	332:341	probiotics	332:341	Supplementation with probiotics may be one of the ways to change the gut microbiota.
34593921	6	9	theme	endurance	1120:1128	arg1	performance					1130:1140	endurance performance	1120:1140	endurance performance	1120:1140	The results showed that 4 weeks of PL-02 supplementation could significantly increase muscle mass, muscle strength and endurance performance, and hepatic and muscular glycogen storage.
34593921	4	10	theme	exercise	722:729	arg1	performance					741:751	improved exercise endurance performance	713:751	improved exercise endurance performance	713:751	In this study, we screened Lactobacillus plantarum (PL-02), a probiotic of human-origin, from the intestines of 2008 Olympic women's 48 kg weightlifting gold medalist and explored the role of PL-02 in improved exercise endurance performance, reduced fatigue biochemical parameters, and changes in body composition.
34593921	5	11	theme	Institute	832:840	arg1	mice					867:870	Male Institute of Cancer Research (ICR) mice	827:870	Male Institute of Cancer Research (ICR) mice	827:870	Male Institute of Cancer Research (ICR) mice were assigned to 0, 2.05 × 109, 4.10 × 109 and 1.03 × 1010 CFU/kg/day groups and were fed by oral gavage once daily for 4 weeks.
34593921	7	12	theme	urea	1249:1252	arg1	BUN					1264:1266	BUN	1264:1266	BUN	1264:1266	Furthermore, PL-02 could significantly decrease lactate, blood urea nitrogen (BUN), ammonia, and creatine kinase (CK) levels after exercise (p < 0.05).
34593921	7	12	theme	urea	1249:1252	arg1	nitrogen					1254:1261	blood urea nitrogen	1243:1261	blood urea nitrogen (BUN)	1243:1267	Furthermore, PL-02 could significantly decrease lactate, blood urea nitrogen (BUN), ammonia, and creatine kinase (CK) levels after exercise (p < 0.05).
34593921	7	13	theme	ammonia	1270:1276	arg1	levels					1304:1309	lactate, blood urea nitrogen (BUN), ammonia, and creatine kinase (CK) levels	1234:1309	lactate, blood urea nitrogen (BUN), ammonia, and creatine kinase (CK) levels	1234:1309	Furthermore, PL-02 could significantly decrease lactate, blood urea nitrogen (BUN), ammonia, and creatine kinase (CK) levels after exercise (p < 0.05).
34593921	2	14	theme	gut	380:382	arg1	microbiota					384:393	the gut microbiota	376:393	the gut microbiota	376:393	Supplementation with probiotics may be one of the ways to change the gut microbiota.
34593921	4	15	theme	body	809:812	arg1	composition					814:824	body composition	809:824	body composition	809:824	In this study, we screened Lactobacillus plantarum (PL-02), a probiotic of human-origin, from the intestines of 2008 Olympic women's 48 kg weightlifting gold medalist and explored the role of PL-02 in improved exercise endurance performance, reduced fatigue biochemical parameters, and changes in body composition.
34593921	5	16	theme	of	842:843	arg1	mice					867:870	Male Institute of Cancer Research (ICR) mice	827:870	Male Institute of Cancer Research (ICR) mice	827:870	Male Institute of Cancer Research (ICR) mice were assigned to 0, 2.05 × 109, 4.10 × 109 and 1.03 × 1010 CFU/kg/day groups and were fed by oral gavage once daily for 4 weeks.
34593921	8	17	used	used	1367:1370	arg2	PL-02					1354:1358	PL-02	1354:1358	PL-02	1354:1358	We believe that PL-02 can be used as a supplement to improve exercise performance and for its anti-fatigue effect.
34593921	8	17	used	used	1367:1370	arg2	supplement					1377:1386	a supplement	1375:1386	a supplement to improve exercise performance	1375:1418	We believe that PL-02 can be used as a supplement to improve exercise performance and for its anti-fatigue effect.
34593921	7	18	theme	lactate	1234:1240	arg1	levels					1304:1309	lactate, blood urea nitrogen (BUN), ammonia, and creatine kinase (CK) levels	1234:1309	lactate, blood urea nitrogen (BUN), ammonia, and creatine kinase (CK) levels	1234:1309	Furthermore, PL-02 could significantly decrease lactate, blood urea nitrogen (BUN), ammonia, and creatine kinase (CK) levels after exercise (p < 0.05).
34593921	4	19	theme	PL-02	704:708	arg1	role					696:699	the role	692:699	the role of PL-02 in improved exercise endurance performance, reduced fatigue biochemical parameters, and changes in body composition	692:824	In this study, we screened Lactobacillus plantarum (PL-02), a probiotic of human-origin, from the intestines of 2008 Olympic women's 48 kg weightlifting gold medalist and explored the role of PL-02 in improved exercise endurance performance, reduced fatigue biochemical parameters, and changes in body composition.
34593921	7	20	theme	blood	1243:1247	arg1	BUN					1264:1266	BUN	1264:1266	BUN	1264:1266	Furthermore, PL-02 could significantly decrease lactate, blood urea nitrogen (BUN), ammonia, and creatine kinase (CK) levels after exercise (p < 0.05).
34593921	7	20	theme	blood	1243:1247	arg1	nitrogen					1254:1261	blood urea nitrogen	1243:1261	blood urea nitrogen (BUN)	1243:1267	Furthermore, PL-02 could significantly decrease lactate, blood urea nitrogen (BUN), ammonia, and creatine kinase (CK) levels after exercise (p < 0.05).
34593921	5	21	theme	Cancer	845:850	arg1	mice					867:870	Male Institute of Cancer Research (ICR) mice	827:870	Male Institute of Cancer Research (ICR) mice	827:870	Male Institute of Cancer Research (ICR) mice were assigned to 0, 2.05 × 109, 4.10 × 109 and 1.03 × 1010 CFU/kg/day groups and were fed by oral gavage once daily for 4 weeks.
34593921	5	22	theme	Research	852:859	arg1	mice					867:870	Male Institute of Cancer Research (ICR) mice	827:870	Male Institute of Cancer Research (ICR) mice	827:870	Male Institute of Cancer Research (ICR) mice were assigned to 0, 2.05 × 109, 4.10 × 109 and 1.03 × 1010 CFU/kg/day groups and were fed by oral gavage once daily for 4 weeks.
34593921	0	23	theme	Lactobacillus	30:42	arg1	PL-02					54:58	human-origin Lactobacillus plantarum PL-02	17:58	human-origin Lactobacillus plantarum PL-02	17:58	Effectiveness of human-origin Lactobacillus plantarum PL-02 in improving muscle mass, exercise performance and anti-fatigue.
34593921	7	24	theme	kinase	1292:1297	arg1	levels					1304:1309	lactate, blood urea nitrogen (BUN), ammonia, and creatine kinase (CK) levels	1234:1309	lactate, blood urea nitrogen (BUN), ammonia, and creatine kinase (CK) levels	1234:1309	Furthermore, PL-02 could significantly decrease lactate, blood urea nitrogen (BUN), ammonia, and creatine kinase (CK) levels after exercise (p < 0.05).
34593921	4	25	theme	improved	713:720	arg1	performance					741:751	improved exercise endurance performance	713:751	improved exercise endurance performance	713:751	In this study, we screened Lactobacillus plantarum (PL-02), a probiotic of human-origin, from the intestines of 2008 Olympic women's 48 kg weightlifting gold medalist and explored the role of PL-02 in improved exercise endurance performance, reduced fatigue biochemical parameters, and changes in body composition.
34593921	0	26	theme	human-origin	17:28	arg1	PL-02					54:58	human-origin Lactobacillus plantarum PL-02	17:58	human-origin Lactobacillus plantarum PL-02	17:58	Effectiveness of human-origin Lactobacillus plantarum PL-02 in improving muscle mass, exercise performance and anti-fatigue.
34593921	8	27	theme	anti-fatigue	1432:1443	arg1	effect					1445:1450	its anti-fatigue effect	1428:1450	its anti-fatigue effect	1428:1450	We believe that PL-02 can be used as a supplement to improve exercise performance and for its anti-fatigue effect.
34593921	6	28	theme	hepatic	1147:1153	arg1	storage					1177:1183	hepatic and muscular glycogen storage	1147:1183	hepatic and muscular glycogen storage	1147:1183	The results showed that 4 weeks of PL-02 supplementation could significantly increase muscle mass, muscle strength and endurance performance, and hepatic and muscular glycogen storage.
34593921	6	29	theme	PL-02	1036:1040	arg1	supplementation					1042:1056	PL-02 supplementation	1036:1056	PL-02 supplementation	1036:1056	The results showed that 4 weeks of PL-02 supplementation could significantly increase muscle mass, muscle strength and endurance performance, and hepatic and muscular glycogen storage.
34593921	3	30	theme	probiotic	442:450	arg1	supplementation					452:466	probiotic supplementation	442:466	probiotic supplementation	442:466	In recent years, many studies have shown that probiotic supplementation can effectively improve sports performance.
34593921	0	31	theme	PL-02	54:58	arg1	Effectiveness					0:12	Effectiveness	0:12	Effectiveness of human-origin Lactobacillus plantarum PL-02 in improving muscle mass, exercise performance and anti-fatigue.	0:123	Effectiveness of human-origin Lactobacillus plantarum PL-02 in improving muscle mass, exercise performance and anti-fatigue.
34593921	0	32	theme	muscle	73:78	arg1	mass					80:83	muscle mass	73:83	muscle mass	73:83	Effectiveness of human-origin Lactobacillus plantarum PL-02 in improving muscle mass, exercise performance and anti-fatigue.
34593921	0	33	theme	plantarum	44:52	arg1	PL-02					54:58	human-origin Lactobacillus plantarum PL-02	17:58	human-origin Lactobacillus plantarum PL-02	17:58	Effectiveness of human-origin Lactobacillus plantarum PL-02 in improving muscle mass, exercise performance and anti-fatigue.
34593921	3	34	theme	recent	399:404	arg1	years					406:410	recent years	399:410	recent years	399:410	In recent years, many studies have shown that probiotic supplementation can effectively improve sports performance.
34593921	1	35	dep	health	221:226	arg1	the					217:219	the	217:219	the	217:219	Gut microbiota is very important for energy metabolism and regulation, which in turn affect the health and physiological functions of the host, and provide energy required for exercise.
34593921	5	36	theme	Male	827:830	arg1	mice					867:870	Male Institute of Cancer Research (ICR) mice	827:870	Male Institute of Cancer Research (ICR) mice	827:870	Male Institute of Cancer Research (ICR) mice were assigned to 0, 2.05 × 109, 4.10 × 109 and 1.03 × 1010 CFU/kg/day groups and were fed by oral gavage once daily for 4 weeks.
34593921	3	37	theme	sports	492:497	arg1	performance					499:509	sports performance	492:509	sports performance	492:509	In recent years, many studies have shown that probiotic supplementation can effectively improve sports performance.
34593921	3	38	theme	many	413:416	arg1	studies					418:424	many studies	413:424	many studies	413:424	In recent years, many studies have shown that probiotic supplementation can effectively improve sports performance.
34593921	5	39	theme	daily	982:986	arg1	gavage					970:975	oral gavage	965:975	oral gavage once daily for 4 weeks	965:998	Male Institute of Cancer Research (ICR) mice were assigned to 0, 2.05 × 109, 4.10 × 109 and 1.03 × 1010 CFU/kg/day groups and were fed by oral gavage once daily for 4 weeks.
34593921	4	40	from	changes	798:804	arg1	composition					814:824	body composition	809:824	body composition	809:824	In this study, we screened Lactobacillus plantarum (PL-02), a probiotic of human-origin, from the intestines of 2008 Olympic women's 48 kg weightlifting gold medalist and explored the role of PL-02 in improved exercise endurance performance, reduced fatigue biochemical parameters, and changes in body composition.
34593921	1	41	theme	Gut	125:127	arg1	microbiota					129:138	Gut microbiota	125:138	Gut microbiota	125:138	Gut microbiota is very important for energy metabolism and regulation, which in turn affect the health and physiological functions of the host, and provide energy required for exercise.
34593921	7	42	theme	nitrogen	1254:1261	arg1	levels					1304:1309	lactate, blood urea nitrogen (BUN), ammonia, and creatine kinase (CK) levels	1234:1309	lactate, blood urea nitrogen (BUN), ammonia, and creatine kinase (CK) levels	1234:1309	Furthermore, PL-02 could significantly decrease lactate, blood urea nitrogen (BUN), ammonia, and creatine kinase (CK) levels after exercise (p < 0.05).
34593921	4	43	from	role	696:699	arg1	performance					741:751	improved exercise endurance performance	713:751	improved exercise endurance performance	713:751	In this study, we screened Lactobacillus plantarum (PL-02), a probiotic of human-origin, from the intestines of 2008 Olympic women's 48 kg weightlifting gold medalist and explored the role of PL-02 in improved exercise endurance performance, reduced fatigue biochemical parameters, and changes in body composition.
34593921	4	43	from	role	696:699	arg1	parameters					782:791	reduced fatigue biochemical parameters	754:791	reduced fatigue biochemical parameters	754:791	In this study, we screened Lactobacillus plantarum (PL-02), a probiotic of human-origin, from the intestines of 2008 Olympic women's 48 kg weightlifting gold medalist and explored the role of PL-02 in improved exercise endurance performance, reduced fatigue biochemical parameters, and changes in body composition.
34593921	4	43	from	role	696:699	arg1	changes					798:804	changes	798:804	changes in body composition	798:824	In this study, we screened Lactobacillus plantarum (PL-02), a probiotic of human-origin, from the intestines of 2008 Olympic women's 48 kg weightlifting gold medalist and explored the role of PL-02 in improved exercise endurance performance, reduced fatigue biochemical parameters, and changes in body composition.
34593921	5	44	theme	oral	965:968	arg1	gavage					970:975	oral gavage	965:975	oral gavage once daily for 4 weeks	965:998	Male Institute of Cancer Research (ICR) mice were assigned to 0, 2.05 × 109, 4.10 × 109 and 1.03 × 1010 CFU/kg/day groups and were fed by oral gavage once daily for 4 weeks.
34593921	4	45	theme	fatigue	762:768	arg1	parameters					782:791	reduced fatigue biochemical parameters	754:791	reduced fatigue biochemical parameters	754:791	In this study, we screened Lactobacillus plantarum (PL-02), a probiotic of human-origin, from the intestines of 2008 Olympic women's 48 kg weightlifting gold medalist and explored the role of PL-02 in improved exercise endurance performance, reduced fatigue biochemical parameters, and changes in body composition.
34593921	6	46	theme	muscle	1087:1092	arg1	mass					1094:1097	muscle mass	1087:1097	muscle mass	1087:1097	The results showed that 4 weeks of PL-02 supplementation could significantly increase muscle mass, muscle strength and endurance performance, and hepatic and muscular glycogen storage.
34593921	8	47	theme	exercise	1399:1406	arg1	performance					1408:1418	exercise performance	1399:1418	exercise performance	1399:1418	We believe that PL-02 can be used as a supplement to improve exercise performance and for its anti-fatigue effect.
34593921	4	48	theme	reduced	754:760	arg1	parameters					782:791	reduced fatigue biochemical parameters	754:791	reduced fatigue biochemical parameters	754:791	In this study, we screened Lactobacillus plantarum (PL-02), a probiotic of human-origin, from the intestines of 2008 Olympic women's 48 kg weightlifting gold medalist and explored the role of PL-02 in improved exercise endurance performance, reduced fatigue biochemical parameters, and changes in body composition.
34593921	4	49	from	parameters	782:791	arg1	composition					814:824	body composition	809:824	body composition	809:824	In this study, we screened Lactobacillus plantarum (PL-02), a probiotic of human-origin, from the intestines of 2008 Olympic women's 48 kg weightlifting gold medalist and explored the role of PL-02 in improved exercise endurance performance, reduced fatigue biochemical parameters, and changes in body composition.
34593921	5	50	theme	ICR	862:864	arg1	mice					867:870	Male Institute of Cancer Research (ICR) mice	827:870	Male Institute of Cancer Research (ICR) mice	827:870	Male Institute of Cancer Research (ICR) mice were assigned to 0, 2.05 × 109, 4.10 × 109 and 1.03 × 1010 CFU/kg/day groups and were fed by oral gavage once daily for 4 weeks.
34593921	6	51	theme	muscular	1159:1166	arg1	storage					1177:1183	hepatic and muscular glycogen storage	1147:1183	hepatic and muscular glycogen storage	1147:1183	The results showed that 4 weeks of PL-02 supplementation could significantly increase muscle mass, muscle strength and endurance performance, and hepatic and muscular glycogen storage.
34593921	2	52	theme	ways	361:364	arg1	one					350:352	one	350:352	one	350:352	Supplementation with probiotics may be one of the ways to change the gut microbiota.
34593921	2	52	theme	ways	361:364	arg1	ways					361:364	the ways	357:364	the ways	357:364	Supplementation with probiotics may be one of the ways to change the gut microbiota.
34593921	7	53	theme	creatine	1283:1290	arg1	CK					1300:1301	CK	1300:1301	CK	1300:1301	Furthermore, PL-02 could significantly decrease lactate, blood urea nitrogen (BUN), ammonia, and creatine kinase (CK) levels after exercise (p < 0.05).
34593921	7	53	theme	creatine	1283:1290	arg1	kinase					1292:1297	creatine kinase	1283:1297	creatine kinase (CK)	1283:1302	Furthermore, PL-02 could significantly decrease lactate, blood urea nitrogen (BUN), ammonia, and creatine kinase (CK) levels after exercise (p < 0.05).
34593921	1	54	theme	physiological	232:244	arg1	functions					246:254	physiological functions	232:254	physiological functions	232:254	Gut microbiota is very important for energy metabolism and regulation, which in turn affect the health and physiological functions of the host, and provide energy required for exercise.
34593921	1	55	theme	host	263:266	arg1	health					221:226	health	221:226	health	221:226	Gut microbiota is very important for energy metabolism and regulation, which in turn affect the health and physiological functions of the host, and provide energy required for exercise.
34593921	1	55	theme	host	263:266	arg1	functions					246:254	physiological functions	232:254	physiological functions	232:254	Gut microbiota is very important for energy metabolism and regulation, which in turn affect the health and physiological functions of the host, and provide energy required for exercise.
34593921	6	56	theme	muscle	1100:1105	arg1	strength					1107:1114	muscle strength	1100:1114	muscle strength	1100:1114	The results showed that 4 weeks of PL-02 supplementation could significantly increase muscle mass, muscle strength and endurance performance, and hepatic and muscular glycogen storage.
34593921	4	57	theme	biochemical	770:780	arg1	parameters					782:791	reduced fatigue biochemical parameters	754:791	reduced fatigue biochemical parameters	754:791	In this study, we screened Lactobacillus plantarum (PL-02), a probiotic of human-origin, from the intestines of 2008 Olympic women's 48 kg weightlifting gold medalist and explored the role of PL-02 in improved exercise endurance performance, reduced fatigue biochemical parameters, and changes in body composition.
34593921	4	58	theme	48 kg	645:649	arg1	intestines					610:619	the intestines	606:619	the intestines of 2008 Olympic women's 48 kg weightlifting gold medalist	606:677	In this study, we screened Lactobacillus plantarum (PL-02), a probiotic of human-origin, from the intestines of 2008 Olympic women's 48 kg weightlifting gold medalist and explored the role of PL-02 in improved exercise endurance performance, reduced fatigue biochemical parameters, and changes in body composition.
34593921	4	59	theme	human-origin	587:598	arg1	plantarum					553:561	Lactobacillus plantarum	539:561	Lactobacillus plantarum (PL-02)	539:569	In this study, we screened Lactobacillus plantarum (PL-02), a probiotic of human-origin, from the intestines of 2008 Olympic women's 48 kg weightlifting gold medalist and explored the role of PL-02 in improved exercise endurance performance, reduced fatigue biochemical parameters, and changes in body composition.
34593921	4	59	theme	human-origin	587:598	arg1	probiotic					574:582	probiotic	574:582	probiotic	574:582	In this study, we screened Lactobacillus plantarum (PL-02), a probiotic of human-origin, from the intestines of 2008 Olympic women's 48 kg weightlifting gold medalist and explored the role of PL-02 in improved exercise endurance performance, reduced fatigue biochemical parameters, and changes in body composition.
32830554	4	0	theme	fat	735:737	arg1	mass					739:742	fat mass	735:742	fat mass	735:742	Both prebiotic fibers led to a similar reduction of body weight and fat mass, lower inflammation and improved metabolic parameters.
32830554	2	1	from	fibers	393:398	arg1	mice					403:406	mice	403:406	mice	403:406	To gain insight into their impact on host physiology and gut microbiota, we performed a direct comparison of two specific prebiotic fibers in mice.
32830554	6	2	theme	communities	1122:1132	arg1	growth					1093:1098	the growth	1089:1098	the growth of the same bacterial communities	1089:1132	Interestingly, both fibers had very distinct gut microbial signatures with different short-chain fatty acid profiles, indicating that they do not favor the growth of the same bacterial communities.
32830554	1	3	theme	metabolic	240:248	arg1	functions					250:258	metabolic functions	240:258	metabolic functions	240:258	Dietary fibers are essential components of a balanced diet and have beneficial effects on metabolic functions.
32830554	7	4	theme	gut	1327:1329	arg1	microbes					1331:1338	different gut microbes	1317:1338	different gut microbes	1317:1338	Although the prebiotic potential of different fibers may seem physiologically equivalent, our data show that the underlying mechanisms of action are different, and this by targeting different gut microbes.
32830554	6	5	theme	acid	1040:1043	arg1	profiles					1045:1052	different short-chain fatty acid profiles	1012:1052	different short-chain fatty acid profiles	1012:1052	Interestingly, both fibers had very distinct gut microbial signatures with different short-chain fatty acid profiles, indicating that they do not favor the growth of the same bacterial communities.
32830554	6	6	theme	same	1107:1110	arg1	communities					1122:1132	the same bacterial communities	1103:1132	the same bacterial communities	1103:1132	Interestingly, both fibers had very distinct gut microbial signatures with different short-chain fatty acid profiles, indicating that they do not favor the growth of the same bacterial communities.
32830554	8	7	theme	microbiota	1561:1570	arg1	modulation					1572:1581	specific microbiota modulation	1552:1581	specific microbiota modulation	1552:1581	Altogether, our data provide evidence that beneficial health effects of specific dietary fibers must be documented to be considered a prebiotic and that studies devoted to understanding how structures relate to specific microbiota modulation and metabolic effects are warranted.
32830554	6	8	theme	short-chain	1022:1032	arg1	profiles					1045:1052	different short-chain fatty acid profiles	1012:1052	different short-chain fatty acid profiles	1012:1052	Interestingly, both fibers had very distinct gut microbial signatures with different short-chain fatty acid profiles, indicating that they do not favor the growth of the same bacterial communities.
32830554	4	9	theme	weight	724:729	arg1	reduction					706:714	a similar reduction	696:714	a similar reduction of body weight and fat mass, lower inflammation and improved metabolic parameters	696:796	Both prebiotic fibers led to a similar reduction of body weight and fat mass, lower inflammation and improved metabolic parameters.
32830554	0	10	theme	diet-fed	135:142	arg1	mice					144:147	high-fat diet-fed mice	126:147	high-fat diet-fed mice	126:147	Comparison of the effects of soluble corn fiber and fructooligosaccharides on metabolism, inflammation, and gut microbiome of high-fat diet-fed mice.
32830554	3	11	theme	soluble	641:647	arg1	SCF					661:663	SCF	661:663	SCF	661:663	During an 8-wk follow up, mice fed a high-fat diet (HFD) were compared with mice on a normal diet (basal condition, controls) and to mice fed the HFD but treated with one of the following prebiotics: fructooligosaccharides (FOS) or soluble corn fiber (SCF).
32830554	3	11	theme	soluble	641:647	arg1	fiber					654:658	soluble corn fiber	641:658	soluble corn fiber (SCF)	641:664	During an 8-wk follow up, mice fed a high-fat diet (HFD) were compared with mice on a normal diet (basal condition, controls) and to mice fed the HFD but treated with one of the following prebiotics: fructooligosaccharides (FOS) or soluble corn fiber (SCF).
32830554	4	12	theme	parameters	787:796	arg1	reduction					706:714	a similar reduction	696:714	a similar reduction of body weight and fat mass, lower inflammation and improved metabolic parameters	696:796	Both prebiotic fibers led to a similar reduction of body weight and fat mass, lower inflammation and improved metabolic parameters.
32830554	6	13	theme	microbial	986:994	arg1	signatures					996:1005	very distinct gut microbial signatures	968:1005	very distinct gut microbial signatures	968:1005	Interestingly, both fibers had very distinct gut microbial signatures with different short-chain fatty acid profiles, indicating that they do not favor the growth of the same bacterial communities.
32830554	0	14	theme	gut	108:110	arg1	microbiome					112:121	gut microbiome	108:121	gut microbiome	108:121	Comparison of the effects of soluble corn fiber and fructooligosaccharides on metabolism, inflammation, and gut microbiome of high-fat diet-fed mice.
32830554	6	15	contain	had	964:966	arg1	fibers					957:962	both fibers	952:962	both fibers	952:962	Interestingly, both fibers had very distinct gut microbial signatures with different short-chain fatty acid profiles, indicating that they do not favor the growth of the same bacterial communities.
32830554	6	15	contain	had	964:966	arg2	signatures					996:1005	very distinct gut microbial signatures	968:1005	very distinct gut microbial signatures	968:1005	Interestingly, both fibers had very distinct gut microbial signatures with different short-chain fatty acid profiles, indicating that they do not favor the growth of the same bacterial communities.
32830554	1	16	theme	diet	204:207	arg1	fibers					158:163	Dietary fibers	150:163	Dietary fibers	150:163	Dietary fibers are essential components of a balanced diet and have beneficial effects on metabolic functions.
32830554	1	16	theme	diet	204:207	arg1	components					179:188	essential components	169:188	essential components of a balanced diet	169:207	Dietary fibers are essential components of a balanced diet and have beneficial effects on metabolic functions.
32830554	4	17	theme	inflammation	751:762	arg1	reduction					706:714	a similar reduction	696:714	a similar reduction of body weight and fat mass, lower inflammation and improved metabolic parameters	696:796	Both prebiotic fibers led to a similar reduction of body weight and fat mass, lower inflammation and improved metabolic parameters.
32830554	1	18	from	effects	229:235	arg1	functions					250:258	metabolic functions	240:258	metabolic functions	240:258	Dietary fibers are essential components of a balanced diet and have beneficial effects on metabolic functions.
32830554	3	19	theme	basal	508:512	arg1	condition					514:522	basal condition	508:522	basal condition	508:522	During an 8-wk follow up, mice fed a high-fat diet (HFD) were compared with mice on a normal diet (basal condition, controls) and to mice fed the HFD but treated with one of the following prebiotics: fructooligosaccharides (FOS) or soluble corn fiber (SCF).
32830554	3	19	theme	basal	508:512	arg1	diet					502:505	a normal diet	493:505	a normal diet (basal condition, controls)	493:533	During an 8-wk follow up, mice fed a high-fat diet (HFD) were compared with mice on a normal diet (basal condition, controls) and to mice fed the HFD but treated with one of the following prebiotics: fructooligosaccharides (FOS) or soluble corn fiber (SCF).
32830554	3	20	dep	compared	471:478	arg1	follow					424:429	follow	424:429	follow	424:429	During an 8-wk follow up, mice fed a high-fat diet (HFD) were compared with mice on a normal diet (basal condition, controls) and to mice fed the HFD but treated with one of the following prebiotics: fructooligosaccharides (FOS) or soluble corn fiber (SCF).
32830554	3	20	dep	compared	471:478	arg1	FOS					633:635	FOS	633:635	FOS	633:635	During an 8-wk follow up, mice fed a high-fat diet (HFD) were compared with mice on a normal diet (basal condition, controls) and to mice fed the HFD but treated with one of the following prebiotics: fructooligosaccharides (FOS) or soluble corn fiber (SCF).
32830554	3	20	dep	compared	471:478	arg1	SCF					661:663	SCF	661:663	SCF	661:663	During an 8-wk follow up, mice fed a high-fat diet (HFD) were compared with mice on a normal diet (basal condition, controls) and to mice fed the HFD but treated with one of the following prebiotics: fructooligosaccharides (FOS) or soluble corn fiber (SCF).
32830554	3	20	dep	compared	471:478	arg1	fiber					654:658	soluble corn fiber	641:658	soluble corn fiber (SCF)	641:664	During an 8-wk follow up, mice fed a high-fat diet (HFD) were compared with mice on a normal diet (basal condition, controls) and to mice fed the HFD but treated with one of the following prebiotics: fructooligosaccharides (FOS) or soluble corn fiber (SCF).
32830554	3	20	dep	compared	471:478	arg1	fructooligosaccharides					609:630	fructooligosaccharides	609:630	fructooligosaccharides (FOS)	609:636	During an 8-wk follow up, mice fed a high-fat diet (HFD) were compared with mice on a normal diet (basal condition, controls) and to mice fed the HFD but treated with one of the following prebiotics: fructooligosaccharides (FOS) or soluble corn fiber (SCF).
32830554	4	21	theme	improved	768:775	arg1	parameters					787:796	improved metabolic parameters	768:796	improved metabolic parameters	768:796	Both prebiotic fibers led to a similar reduction of body weight and fat mass, lower inflammation and improved metabolic parameters.
32830554	2	22	from	impact	288:293	arg1	physiology					303:312	host physiology	298:312	host physiology	298:312	To gain insight into their impact on host physiology and gut microbiota, we performed a direct comparison of two specific prebiotic fibers in mice.
32830554	2	22	from	impact	288:293	arg1	microbiota					322:331	gut microbiota	318:331	gut microbiota	318:331	To gain insight into their impact on host physiology and gut microbiota, we performed a direct comparison of two specific prebiotic fibers in mice.
32830554	2	23	from	mice	403:406	arg1	comparison					356:365	a direct comparison	347:365	a direct comparison of two specific prebiotic fibers in mice	347:406	To gain insight into their impact on host physiology and gut microbiota, we performed a direct comparison of two specific prebiotic fibers in mice.
32830554	8	24	dep	prebiotic	1475:1483	arg1	studies					1494:1500	studies	1494:1500	studies	1494:1500	Altogether, our data provide evidence that beneficial health effects of specific dietary fibers must be documented to be considered a prebiotic and that studies devoted to understanding how structures relate to specific microbiota modulation and metabolic effects are warranted.
32830554	1	25	theme	Dietary	150:156	arg1	fibers					158:163	Dietary fibers	150:163	Dietary fibers	150:163	Dietary fibers are essential components of a balanced diet and have beneficial effects on metabolic functions.
32830554	1	25	theme	Dietary	150:156	arg1	components					179:188	essential components	169:188	essential components of a balanced diet	169:207	Dietary fibers are essential components of a balanced diet and have beneficial effects on metabolic functions.
32830554	0	26	from	Comparison	0:9	arg1	inflammation					90:101	inflammation	90:101	inflammation	90:101	Comparison of the effects of soluble corn fiber and fructooligosaccharides on metabolism, inflammation, and gut microbiome of high-fat diet-fed mice.
32830554	0	26	from	Comparison	0:9	arg1	metabolism					78:87	metabolism	78:87	metabolism	78:87	Comparison of the effects of soluble corn fiber and fructooligosaccharides on metabolism, inflammation, and gut microbiome of high-fat diet-fed mice.
32830554	0	26	from	Comparison	0:9	arg1	microbiome					112:121	gut microbiome	108:121	gut microbiome	108:121	Comparison of the effects of soluble corn fiber and fructooligosaccharides on metabolism, inflammation, and gut microbiome of high-fat diet-fed mice.
32830554	3	27	from	mice	485:488	arg1	condition					514:522	basal condition	508:522	basal condition	508:522	During an 8-wk follow up, mice fed a high-fat diet (HFD) were compared with mice on a normal diet (basal condition, controls) and to mice fed the HFD but treated with one of the following prebiotics: fructooligosaccharides (FOS) or soluble corn fiber (SCF).
32830554	3	27	from	mice	485:488	arg1	diet					502:505	a normal diet	493:505	a normal diet (basal condition, controls)	493:533	During an 8-wk follow up, mice fed a high-fat diet (HFD) were compared with mice on a normal diet (basal condition, controls) and to mice fed the HFD but treated with one of the following prebiotics: fructooligosaccharides (FOS) or soluble corn fiber (SCF).
32830554	7	28	theme	different	1171:1179	arg1	fibers					1181:1186	different fibers	1171:1186	different fibers	1171:1186	Although the prebiotic potential of different fibers may seem physiologically equivalent, our data show that the underlying mechanisms of action are different, and this by targeting different gut microbes.
32830554	8	29	theme	health	1395:1400	arg1	effects					1402:1408	beneficial health effects	1384:1408	beneficial health effects of specific dietary fibers	1384:1435	Altogether, our data provide evidence that beneficial health effects of specific dietary fibers must be documented to be considered a prebiotic and that studies devoted to understanding how structures relate to specific microbiota modulation and metabolic effects are warranted.
32830554	7	30	theme	action	1273:1278	arg1	mechanisms					1259:1268	the underlying mechanisms	1244:1268	the underlying mechanisms of action	1244:1278	Although the prebiotic potential of different fibers may seem physiologically equivalent, our data show that the underlying mechanisms of action are different, and this by targeting different gut microbes.
32830554	7	30	theme	action	1273:1278	arg1	different					1284:1292	different	1284:1292	different	1284:1292	Although the prebiotic potential of different fibers may seem physiologically equivalent, our data show that the underlying mechanisms of action are different, and this by targeting different gut microbes.
32830554	2	31	theme	prebiotic	383:391	arg1	fibers					393:398	two specific prebiotic fibers	370:398	two specific prebiotic fibers in mice	370:406	To gain insight into their impact on host physiology and gut microbiota, we performed a direct comparison of two specific prebiotic fibers in mice.
32830554	2	32	from	comparison	356:365	arg1	mice					403:406	mice	403:406	mice	403:406	To gain insight into their impact on host physiology and gut microbiota, we performed a direct comparison of two specific prebiotic fibers in mice.
32830554	8	33	theme	dietary	1422:1428	arg1	fibers					1430:1435	specific dietary fibers	1413:1435	specific dietary fibers	1413:1435	Altogether, our data provide evidence that beneficial health effects of specific dietary fibers must be documented to be considered a prebiotic and that studies devoted to understanding how structures relate to specific microbiota modulation and metabolic effects are warranted.
32830554	4	34	theme	similar	698:704	arg1	reduction					706:714	a similar reduction	696:714	a similar reduction of body weight and fat mass, lower inflammation and improved metabolic parameters	696:796	Both prebiotic fibers led to a similar reduction of body weight and fat mass, lower inflammation and improved metabolic parameters.
32830554	0	35	theme	corn	37:40	arg1	fiber					42:46	soluble corn fiber	29:46	soluble corn fiber	29:46	Comparison of the effects of soluble corn fiber and fructooligosaccharides on metabolism, inflammation, and gut microbiome of high-fat diet-fed mice.
32830554	3	36	dep	mice	542:545	arg1	treated					563:569	treated	563:569	treated with one of the following prebiotics	563:606	During an 8-wk follow up, mice fed a high-fat diet (HFD) were compared with mice on a normal diet (basal condition, controls) and to mice fed the HFD but treated with one of the following prebiotics: fructooligosaccharides (FOS) or soluble corn fiber (SCF).
32830554	3	36	dep	mice	542:545	arg1	fed					547:549	fed	547:549	fed the HFD	547:557	During an 8-wk follow up, mice fed a high-fat diet (HFD) were compared with mice on a normal diet (basal condition, controls) and to mice fed the HFD but treated with one of the following prebiotics: fructooligosaccharides (FOS) or soluble corn fiber (SCF).
32830554	5	37	theme	actions	859:865	arg1	benefits					821:828	these health benefits	808:828	these health benefits	808:828	However, these health benefits were the result of different actions of the fibers, as SCF impacted energy excretion, whereas FOS did not.
32830554	5	37	theme	actions	859:865	arg1	result					839:844	the result	835:844	the result of different actions of the fibers	835:879	However, these health benefits were the result of different actions of the fibers, as SCF impacted energy excretion, whereas FOS did not.
32830554	1	38	theme	beneficial	218:227	arg1	effects					229:235	beneficial effects	218:235	beneficial effects on metabolic functions	218:258	Dietary fibers are essential components of a balanced diet and have beneficial effects on metabolic functions.
32830554	3	39	theme	prebiotics	597:606	arg1	prebiotics					597:606	the following prebiotics	583:606	the following prebiotics	583:606	During an 8-wk follow up, mice fed a high-fat diet (HFD) were compared with mice on a normal diet (basal condition, controls) and to mice fed the HFD but treated with one of the following prebiotics: fructooligosaccharides (FOS) or soluble corn fiber (SCF).
32830554	3	39	theme	prebiotics	597:606	arg1	one					576:578	one	576:578	one	576:578	During an 8-wk follow up, mice fed a high-fat diet (HFD) were compared with mice on a normal diet (basal condition, controls) and to mice fed the HFD but treated with one of the following prebiotics: fructooligosaccharides (FOS) or soluble corn fiber (SCF).
32830554	7	40	theme	different	1317:1325	arg1	microbes					1331:1338	different gut microbes	1317:1338	different gut microbes	1317:1338	Although the prebiotic potential of different fibers may seem physiologically equivalent, our data show that the underlying mechanisms of action are different, and this by targeting different gut microbes.
32830554	6	41	theme	bacterial	1112:1120	arg1	communities					1122:1132	the same bacterial communities	1103:1132	the same bacterial communities	1103:1132	Interestingly, both fibers had very distinct gut microbial signatures with different short-chain fatty acid profiles, indicating that they do not favor the growth of the same bacterial communities.
32830554	2	42	theme	host	298:301	arg1	physiology					303:312	host physiology	298:312	host physiology	298:312	To gain insight into their impact on host physiology and gut microbiota, we performed a direct comparison of two specific prebiotic fibers in mice.
32830554	4	43	theme	mass	739:742	arg1	reduction					706:714	a similar reduction	696:714	a similar reduction of body weight and fat mass, lower inflammation and improved metabolic parameters	696:796	Both prebiotic fibers led to a similar reduction of body weight and fat mass, lower inflammation and improved metabolic parameters.
32830554	6	44	theme	fatty	1034:1038	arg1	profiles					1045:1052	different short-chain fatty acid profiles	1012:1052	different short-chain fatty acid profiles	1012:1052	Interestingly, both fibers had very distinct gut microbial signatures with different short-chain fatty acid profiles, indicating that they do not favor the growth of the same bacterial communities.
32830554	8	45	theme	specific	1552:1559	arg1	modulation					1572:1581	specific microbiota modulation	1552:1581	specific microbiota modulation	1552:1581	Altogether, our data provide evidence that beneficial health effects of specific dietary fibers must be documented to be considered a prebiotic and that studies devoted to understanding how structures relate to specific microbiota modulation and metabolic effects are warranted.
32830554	6	46	theme	different	1012:1020	arg1	profiles					1045:1052	different short-chain fatty acid profiles	1012:1052	different short-chain fatty acid profiles	1012:1052	Interestingly, both fibers had very distinct gut microbial signatures with different short-chain fatty acid profiles, indicating that they do not favor the growth of the same bacterial communities.
32830554	3	47	theme	normal	495:500	arg1	condition					514:522	basal condition	508:522	basal condition	508:522	During an 8-wk follow up, mice fed a high-fat diet (HFD) were compared with mice on a normal diet (basal condition, controls) and to mice fed the HFD but treated with one of the following prebiotics: fructooligosaccharides (FOS) or soluble corn fiber (SCF).
32830554	3	47	theme	normal	495:500	arg1	diet					502:505	a normal diet	493:505	a normal diet (basal condition, controls)	493:533	During an 8-wk follow up, mice fed a high-fat diet (HFD) were compared with mice on a normal diet (basal condition, controls) and to mice fed the HFD but treated with one of the following prebiotics: fructooligosaccharides (FOS) or soluble corn fiber (SCF).
32830554	3	48	dep	condition	514:522	arg1	controls					525:532	controls	525:532	controls	525:532	During an 8-wk follow up, mice fed a high-fat diet (HFD) were compared with mice on a normal diet (basal condition, controls) and to mice fed the HFD but treated with one of the following prebiotics: fructooligosaccharides (FOS) or soluble corn fiber (SCF).
32830554	4	49	theme	body	719:722	arg1	weight					724:729	body weight	719:729	body weight	719:729	Both prebiotic fibers led to a similar reduction of body weight and fat mass, lower inflammation and improved metabolic parameters.
32830554	3	50	theme	corn	649:652	arg1	SCF					661:663	SCF	661:663	SCF	661:663	During an 8-wk follow up, mice fed a high-fat diet (HFD) were compared with mice on a normal diet (basal condition, controls) and to mice fed the HFD but treated with one of the following prebiotics: fructooligosaccharides (FOS) or soluble corn fiber (SCF).
32830554	3	50	theme	corn	649:652	arg1	fiber					654:658	soluble corn fiber	641:658	soluble corn fiber (SCF)	641:664	During an 8-wk follow up, mice fed a high-fat diet (HFD) were compared with mice on a normal diet (basal condition, controls) and to mice fed the HFD but treated with one of the following prebiotics: fructooligosaccharides (FOS) or soluble corn fiber (SCF).
32830554	8	51	theme	metabolic	1587:1595	arg1	effects					1597:1603	metabolic effects	1587:1603	metabolic effects	1587:1603	Altogether, our data provide evidence that beneficial health effects of specific dietary fibers must be documented to be considered a prebiotic and that studies devoted to understanding how structures relate to specific microbiota modulation and metabolic effects are warranted.
32830554	1	52	theme	essential	169:177	arg1	fibers					158:163	Dietary fibers	150:163	Dietary fibers	150:163	Dietary fibers are essential components of a balanced diet and have beneficial effects on metabolic functions.
32830554	1	52	theme	essential	169:177	arg1	components					179:188	essential components	169:188	essential components of a balanced diet	169:207	Dietary fibers are essential components of a balanced diet and have beneficial effects on metabolic functions.
32830554	4	53	theme	metabolic	777:785	arg1	parameters					787:796	improved metabolic parameters	768:796	improved metabolic parameters	768:796	Both prebiotic fibers led to a similar reduction of body weight and fat mass, lower inflammation and improved metabolic parameters.
32830554	0	54	from	metabolism	78:87	arg1	Comparison					0:9	Comparison	0:9	Comparison of the effects of soluble corn fiber and fructooligosaccharides on metabolism, inflammation, and gut microbiome of high-fat diet-fed mice.	0:148	Comparison of the effects of soluble corn fiber and fructooligosaccharides on metabolism, inflammation, and gut microbiome of high-fat diet-fed mice.
32830554	6	55	theme	gut	982:984	arg1	signatures					996:1005	very distinct gut microbial signatures	968:1005	very distinct gut microbial signatures	968:1005	Interestingly, both fibers had very distinct gut microbial signatures with different short-chain fatty acid profiles, indicating that they do not favor the growth of the same bacterial communities.
32830554	2	56	theme	gut	318:320	arg1	microbiota					322:331	gut microbiota	318:331	gut microbiota	318:331	To gain insight into their impact on host physiology and gut microbiota, we performed a direct comparison of two specific prebiotic fibers in mice.
32830554	0	57	theme	high-fat	126:133	arg1	mice					144:147	high-fat diet-fed mice	126:147	high-fat diet-fed mice	126:147	Comparison of the effects of soluble corn fiber and fructooligosaccharides on metabolism, inflammation, and gut microbiome of high-fat diet-fed mice.
32830554	1	58	theme	balanced	195:202	arg1	diet					204:207	a balanced diet	193:207	a balanced diet	193:207	Dietary fibers are essential components of a balanced diet and have beneficial effects on metabolic functions.
32830554	0	59	theme	mice	144:147	arg1	inflammation					90:101	inflammation	90:101	inflammation	90:101	Comparison of the effects of soluble corn fiber and fructooligosaccharides on metabolism, inflammation, and gut microbiome of high-fat diet-fed mice.
32830554	0	59	theme	mice	144:147	arg1	metabolism					78:87	metabolism	78:87	metabolism	78:87	Comparison of the effects of soluble corn fiber and fructooligosaccharides on metabolism, inflammation, and gut microbiome of high-fat diet-fed mice.
32830554	0	59	theme	mice	144:147	arg1	microbiome					112:121	gut microbiome	108:121	gut microbiome	108:121	Comparison of the effects of soluble corn fiber and fructooligosaccharides on metabolism, inflammation, and gut microbiome of high-fat diet-fed mice.
32830554	1	60	contain	have	213:216	arg1	fibers					158:163	Dietary fibers	150:163	Dietary fibers	150:163	Dietary fibers are essential components of a balanced diet and have beneficial effects on metabolic functions.
32830554	1	60	contain	have	213:216	arg1	components					179:188	essential components	169:188	essential components of a balanced diet	169:207	Dietary fibers are essential components of a balanced diet and have beneficial effects on metabolic functions.
32830554	1	60	contain	have	213:216	arg2	effects					229:235	beneficial effects	218:235	beneficial effects on metabolic functions	218:258	Dietary fibers are essential components of a balanced diet and have beneficial effects on metabolic functions.
32830554	4	61	theme	lower	745:749	arg1	inflammation					751:762	lower inflammation	745:762	lower inflammation	745:762	Both prebiotic fibers led to a similar reduction of body weight and fat mass, lower inflammation and improved metabolic parameters.
32830554	5	62	theme	energy	898:903	arg1	excretion					905:913	energy excretion	898:913	energy excretion	898:913	However, these health benefits were the result of different actions of the fibers, as SCF impacted energy excretion, whereas FOS did not.
32830554	0	63	from	inflammation	90:101	arg1	Comparison					0:9	Comparison	0:9	Comparison of the effects of soluble corn fiber and fructooligosaccharides on metabolism, inflammation, and gut microbiome of high-fat diet-fed mice.	0:148	Comparison of the effects of soluble corn fiber and fructooligosaccharides on metabolism, inflammation, and gut microbiome of high-fat diet-fed mice.
32830554	8	64	dep	understanding	1513:1525	arg1	relate					1542:1547	relate	1542:1547	relate to specific microbiota modulation	1542:1581	Altogether, our data provide evidence that beneficial health effects of specific dietary fibers must be documented to be considered a prebiotic and that studies devoted to understanding how structures relate to specific microbiota modulation and metabolic effects are warranted.
32830554	8	64	dep	understanding	1513:1525	arg1	warranted					1609:1617	warranted	1609:1617	are warranted	1605:1617	Altogether, our data provide evidence that beneficial health effects of specific dietary fibers must be documented to be considered a prebiotic and that studies devoted to understanding how structures relate to specific microbiota modulation and metabolic effects are warranted.
32830554	0	65	theme	fructooligosaccharides	52:73	arg1	effects					18:24	the effects	14:24	the effects of soluble corn fiber and fructooligosaccharides on metabolism, inflammation, and gut microbiome of high-fat diet-fed mice	14:147	Comparison of the effects of soluble corn fiber and fructooligosaccharides on metabolism, inflammation, and gut microbiome of high-fat diet-fed mice.
32830554	0	66	from	effects	18:24	arg1	inflammation					90:101	inflammation	90:101	inflammation	90:101	Comparison of the effects of soluble corn fiber and fructooligosaccharides on metabolism, inflammation, and gut microbiome of high-fat diet-fed mice.
32830554	0	66	from	effects	18:24	arg1	metabolism					78:87	metabolism	78:87	metabolism	78:87	Comparison of the effects of soluble corn fiber and fructooligosaccharides on metabolism, inflammation, and gut microbiome of high-fat diet-fed mice.
32830554	0	66	from	effects	18:24	arg1	microbiome					112:121	gut microbiome	108:121	gut microbiome	108:121	Comparison of the effects of soluble corn fiber and fructooligosaccharides on metabolism, inflammation, and gut microbiome of high-fat diet-fed mice.
32830554	7	67	theme	underlying	1248:1257	arg1	mechanisms					1259:1268	the underlying mechanisms	1244:1268	the underlying mechanisms of action	1244:1278	Although the prebiotic potential of different fibers may seem physiologically equivalent, our data show that the underlying mechanisms of action are different, and this by targeting different gut microbes.
32830554	7	67	theme	underlying	1248:1257	arg1	different					1284:1292	different	1284:1292	different	1284:1292	Although the prebiotic potential of different fibers may seem physiologically equivalent, our data show that the underlying mechanisms of action are different, and this by targeting different gut microbes.
32830554	7	68	theme	fibers	1181:1186	arg1	potential					1158:1166	the prebiotic potential	1144:1166	the prebiotic potential of different fibers	1144:1186	Although the prebiotic potential of different fibers may seem physiologically equivalent, our data show that the underlying mechanisms of action are different, and this by targeting different gut microbes.
32830554	8	69	theme	beneficial	1384:1393	arg1	effects					1402:1408	beneficial health effects	1384:1408	beneficial health effects of specific dietary fibers	1384:1435	Altogether, our data provide evidence that beneficial health effects of specific dietary fibers must be documented to be considered a prebiotic and that studies devoted to understanding how structures relate to specific microbiota modulation and metabolic effects are warranted.
32830554	2	70	theme	direct	349:354	arg1	comparison					356:365	a direct comparison	347:365	a direct comparison of two specific prebiotic fibers in mice	347:406	To gain insight into their impact on host physiology and gut microbiota, we performed a direct comparison of two specific prebiotic fibers in mice.
32830554	0	71	theme	effects	18:24	arg1	Comparison					0:9	Comparison	0:9	Comparison of the effects of soluble corn fiber and fructooligosaccharides on metabolism, inflammation, and gut microbiome of high-fat diet-fed mice.	0:148	Comparison of the effects of soluble corn fiber and fructooligosaccharides on metabolism, inflammation, and gut microbiome of high-fat diet-fed mice.
32830554	5	72	theme	health	814:819	arg1	benefits					821:828	these health benefits	808:828	these health benefits	808:828	However, these health benefits were the result of different actions of the fibers, as SCF impacted energy excretion, whereas FOS did not.
32830554	5	72	theme	health	814:819	arg1	result					839:844	the result	835:844	the result of different actions of the fibers	835:879	However, these health benefits were the result of different actions of the fibers, as SCF impacted energy excretion, whereas FOS did not.
32830554	7	73	theme	prebiotic	1148:1156	arg1	potential					1158:1166	the prebiotic potential	1144:1166	the prebiotic potential of different fibers	1144:1186	Although the prebiotic potential of different fibers may seem physiologically equivalent, our data show that the underlying mechanisms of action are different, and this by targeting different gut microbes.
32830554	8	74	theme	specific	1413:1420	arg1	fibers					1430:1435	specific dietary fibers	1413:1435	specific dietary fibers	1413:1435	Altogether, our data provide evidence that beneficial health effects of specific dietary fibers must be documented to be considered a prebiotic and that studies devoted to understanding how structures relate to specific microbiota modulation and metabolic effects are warranted.
32830554	2	75	theme	fibers	393:398	arg1	comparison					356:365	a direct comparison	347:365	a direct comparison of two specific prebiotic fibers in mice	347:406	To gain insight into their impact on host physiology and gut microbiota, we performed a direct comparison of two specific prebiotic fibers in mice.
32830554	0	76	theme	soluble	29:35	arg1	fiber					42:46	soluble corn fiber	29:46	soluble corn fiber	29:46	Comparison of the effects of soluble corn fiber and fructooligosaccharides on metabolism, inflammation, and gut microbiome of high-fat diet-fed mice.
32830554	5	77	theme	fibers	874:879	arg1	actions					859:865	different actions	849:865	different actions of the fibers	849:879	However, these health benefits were the result of different actions of the fibers, as SCF impacted energy excretion, whereas FOS did not.
32830554	8	78	theme	fibers	1430:1435	arg1	effects					1402:1408	beneficial health effects	1384:1408	beneficial health effects of specific dietary fibers	1384:1435	Altogether, our data provide evidence that beneficial health effects of specific dietary fibers must be documented to be considered a prebiotic and that studies devoted to understanding how structures relate to specific microbiota modulation and metabolic effects are warranted.
32830554	2	79	theme	specific	374:381	arg1	fibers					393:398	two specific prebiotic fibers	370:398	two specific prebiotic fibers in mice	370:406	To gain insight into their impact on host physiology and gut microbiota, we performed a direct comparison of two specific prebiotic fibers in mice.
32830554	0	80	theme	fiber	42:46	arg1	effects					18:24	the effects	14:24	the effects of soluble corn fiber and fructooligosaccharides on metabolism, inflammation, and gut microbiome of high-fat diet-fed mice	14:147	Comparison of the effects of soluble corn fiber and fructooligosaccharides on metabolism, inflammation, and gut microbiome of high-fat diet-fed mice.
32830554	6	81	theme	distinct	973:980	arg1	signatures					996:1005	very distinct gut microbial signatures	968:1005	very distinct gut microbial signatures	968:1005	Interestingly, both fibers had very distinct gut microbial signatures with different short-chain fatty acid profiles, indicating that they do not favor the growth of the same bacterial communities.
32830554	3	82	theme	high-fat	446:453	arg1	HFD					461:463	HFD	461:463	HFD	461:463	During an 8-wk follow up, mice fed a high-fat diet (HFD) were compared with mice on a normal diet (basal condition, controls) and to mice fed the HFD but treated with one of the following prebiotics: fructooligosaccharides (FOS) or soluble corn fiber (SCF).
32830554	3	82	theme	high-fat	446:453	arg1	diet					455:458	a high-fat diet	444:458	a high-fat diet (HFD)	444:464	During an 8-wk follow up, mice fed a high-fat diet (HFD) were compared with mice on a normal diet (basal condition, controls) and to mice fed the HFD but treated with one of the following prebiotics: fructooligosaccharides (FOS) or soluble corn fiber (SCF).
32830554	5	83	theme	different	849:857	arg1	actions					859:865	different actions	849:865	different actions of the fibers	849:879	However, these health benefits were the result of different actions of the fibers, as SCF impacted energy excretion, whereas FOS did not.
32830554	0	84	from	microbiome	112:121	arg1	Comparison					0:9	Comparison	0:9	Comparison of the effects of soluble corn fiber and fructooligosaccharides on metabolism, inflammation, and gut microbiome of high-fat diet-fed mice.	0:148	Comparison of the effects of soluble corn fiber and fructooligosaccharides on metabolism, inflammation, and gut microbiome of high-fat diet-fed mice.
32830554	3	85	theme	following	587:595	arg1	prebiotics					597:606	the following prebiotics	583:606	the following prebiotics	583:606	During an 8-wk follow up, mice fed a high-fat diet (HFD) were compared with mice on a normal diet (basal condition, controls) and to mice fed the HFD but treated with one of the following prebiotics: fructooligosaccharides (FOS) or soluble corn fiber (SCF).
32830554	4	86	theme	prebiotic	672:680	arg1	fibers					682:687	Both prebiotic fibers	667:687	Both prebiotic fibers	667:687	Both prebiotic fibers led to a similar reduction of body weight and fat mass, lower inflammation and improved metabolic parameters.
32272467	7	0	theme	microparticle-impregnated	1082:1106	arg1	filler					1126:1131	methanolic extract-blended chitosan microparticle-impregnated calcium phosphate filler	1046:1131	methanolic extract-blended chitosan microparticle-impregnated calcium phosphate filler	1046:1131	On the basis of the desirable results obtained, we provide a preliminary rationale for the use of methanolic extract-blended chitosan microparticle-impregnated calcium phosphate filler for enhanced bone regeneration.
32272467	1	1	theme	traditional	174:184	arg1	medicine					186:193	Indian traditional medicine	167:193	Indian traditional medicine	167:193	Withania somnifera (ashwagandha) is used in Indian traditional medicine for its various health benefits.
32272467	4	2	theme	pre-osteoblasts	649:663	arg1	proliferation					612:624	proliferation	612:624	proliferation	612:624	The controlled release of bioactive molecules enabled enhanced proliferation and differentiation of pre-osteoblasts.
32272467	4	2	theme	pre-osteoblasts	649:663	arg1	differentiation					630:644	differentiation	630:644	differentiation	630:644	The controlled release of bioactive molecules enabled enhanced proliferation and differentiation of pre-osteoblasts.
32272467	0	3	theme	somnifera	103:111	arg1	extracts					113:120	Withania somnifera extracts	94:120	Withania somnifera extracts	94:120	Enhanced bone mineralization using hydroxyapatite-based ceramic bone substitute incorporating Withania somnifera extracts.
32272467	7	4	theme	methanolic	1046:1055	arg1	filler					1126:1131	methanolic extract-blended chitosan microparticle-impregnated calcium phosphate filler	1046:1131	methanolic extract-blended chitosan microparticle-impregnated calcium phosphate filler	1046:1131	On the basis of the desirable results obtained, we provide a preliminary rationale for the use of methanolic extract-blended chitosan microparticle-impregnated calcium phosphate filler for enhanced bone regeneration.
32272467	2	5	attach	present	262:268	arg2	Withaferin-A					228:239	Withaferin-A	228:239	Withaferin-A	228:239	Withaferin-A, a steroidal lactone present in this herb, has shown proteosomal inhibition-based enhancement of bone mineralization.
32272467	2	5	attach	present	262:268	arg1	herb					278:281	this herb	273:281	this herb	273:281	Withaferin-A, a steroidal lactone present in this herb, has shown proteosomal inhibition-based enhancement of bone mineralization.
32272467	2	5	attach	present	262:268	arg2	lactone					254:260	a steroidal lactone	242:260	a steroidal lactone present in this herb	242:281	Withaferin-A, a steroidal lactone present in this herb, has shown proteosomal inhibition-based enhancement of bone mineralization.
32272467	3	6	theme	chitosan	380:387	arg1	porogen					486:492	a porogen	484:492	a porogen in calcium phosphate-based hydroxyapatite bone filler	484:546	In the present work, chitosan microparticles blended with total methanolic root extract of W. somnifera were incorporated as a porogen in calcium phosphate-based hydroxyapatite bone filler.
32272467	3	6	theme	chitosan	380:387	arg1	microparticles					389:402	chitosan microparticles	380:402	chitosan microparticles blended with total methanolic root extract of W. somnifera	380:461	In the present work, chitosan microparticles blended with total methanolic root extract of W. somnifera were incorporated as a porogen in calcium phosphate-based hydroxyapatite bone filler.
32272467	4	7	theme	controlled	553:562	arg1	release					564:570	The controlled release	549:570	The controlled release of bioactive molecules	549:593	The controlled release of bioactive molecules enabled enhanced proliferation and differentiation of pre-osteoblasts.
32272467	5	8	theme	setting	739:745	arg1	time					747:750	the setting time	735:750	the setting time	735:750	Microparticle percentages were optimized to have a minimum effect on the setting time, mechanical strength and degradability of hydroxyapatite bone filler.
32272467	7	9	theme	extract-blended	1057:1071	arg1	filler					1126:1131	methanolic extract-blended chitosan microparticle-impregnated calcium phosphate filler	1046:1131	methanolic extract-blended chitosan microparticle-impregnated calcium phosphate filler	1046:1131	On the basis of the desirable results obtained, we provide a preliminary rationale for the use of methanolic extract-blended chitosan microparticle-impregnated calcium phosphate filler for enhanced bone regeneration.
32272467	2	10	theme	inhibition-based	306:321	arg1	enhancement					323:333	proteosomal inhibition-based enhancement	294:333	proteosomal inhibition-based enhancement of bone mineralization	294:356	Withaferin-A, a steroidal lactone present in this herb, has shown proteosomal inhibition-based enhancement of bone mineralization.
32272467	7	11	theme	desirable	968:976	arg1	results					978:984	the desirable results	964:984	the desirable results obtained	964:993	On the basis of the desirable results obtained, we provide a preliminary rationale for the use of methanolic extract-blended chitosan microparticle-impregnated calcium phosphate filler for enhanced bone regeneration.
32272467	2	12	theme	mineralization	343:356	arg1	enhancement					323:333	proteosomal inhibition-based enhancement	294:333	proteosomal inhibition-based enhancement of bone mineralization	294:356	Withaferin-A, a steroidal lactone present in this herb, has shown proteosomal inhibition-based enhancement of bone mineralization.
32272467	7	13	theme	bone	1146:1149	arg1	regeneration					1151:1162	enhanced bone regeneration	1137:1162	enhanced bone regeneration	1137:1162	On the basis of the desirable results obtained, we provide a preliminary rationale for the use of methanolic extract-blended chitosan microparticle-impregnated calcium phosphate filler for enhanced bone regeneration.
32272467	2	14	theme	proteosomal	294:304	arg1	enhancement					323:333	proteosomal inhibition-based enhancement	294:333	proteosomal inhibition-based enhancement of bone mineralization	294:356	Withaferin-A, a steroidal lactone present in this herb, has shown proteosomal inhibition-based enhancement of bone mineralization.
32272467	5	15	contain	have	710:713	arg2	effect					725:730	a minimum effect	715:730	a minimum effect	715:730	Microparticle percentages were optimized to have a minimum effect on the setting time, mechanical strength and degradability of hydroxyapatite bone filler.
32272467	5	15	contain	have	710:713	arg1	percentages					680:690	Microparticle percentages	666:690	Microparticle percentages	666:690	Microparticle percentages were optimized to have a minimum effect on the setting time, mechanical strength and degradability of hydroxyapatite bone filler.
32272467	3	16	theme	somnifera	453:461	arg1	extract					439:445	total methanolic root extract	417:445	total methanolic root extract of W. somnifera	417:461	In the present work, chitosan microparticles blended with total methanolic root extract of W. somnifera were incorporated as a porogen in calcium phosphate-based hydroxyapatite bone filler.
32272467	3	17	theme	methanolic	423:432	arg1	extract					439:445	total methanolic root extract	417:445	total methanolic root extract of W. somnifera	417:461	In the present work, chitosan microparticles blended with total methanolic root extract of W. somnifera were incorporated as a porogen in calcium phosphate-based hydroxyapatite bone filler.
32272467	2	18	from	present	262:268	arg1	herb					278:281	this herb	273:281	this herb	273:281	Withaferin-A, a steroidal lactone present in this herb, has shown proteosomal inhibition-based enhancement of bone mineralization.
32272467	0	19	theme	bone	9:12	arg1	mineralization					14:27	Enhanced bone mineralization	0:27	Enhanced bone mineralization using hydroxyapatite-based ceramic bone	0:67	Enhanced bone mineralization using hydroxyapatite-based ceramic bone substitute incorporating Withania somnifera extracts.
32272467	5	20	theme	minimum	717:723	arg1	effect					725:730	a minimum effect	715:730	a minimum effect	715:730	Microparticle percentages were optimized to have a minimum effect on the setting time, mechanical strength and degradability of hydroxyapatite bone filler.
32272467	1	21	used	used	159:162	arg2	ashwagandha					143:153	ashwagandha	143:153	ashwagandha	143:153	Withania somnifera (ashwagandha) is used in Indian traditional medicine for its various health benefits.
32272467	1	21	used	used	159:162	arg2	somnifera					132:140	Withania somnifera	123:140	Withania somnifera (ashwagandha)	123:154	Withania somnifera (ashwagandha) is used in Indian traditional medicine for its various health benefits.
32272467	1	22	theme	various	203:209	arg1	benefits					218:225	its various health benefits	199:225	its various health benefits	199:225	Withania somnifera (ashwagandha) is used in Indian traditional medicine for its various health benefits.
32272467	0	23	theme	Enhanced	0:7	arg1	mineralization					14:27	Enhanced bone mineralization	0:27	Enhanced bone mineralization using hydroxyapatite-based ceramic bone	0:67	Enhanced bone mineralization using hydroxyapatite-based ceramic bone substitute incorporating Withania somnifera extracts.
32272467	7	24	theme	preliminary	1009:1019	arg1	rationale					1021:1029	a preliminary rationale	1007:1029	a preliminary rationale for the use of methanolic extract-blended chitosan microparticle-impregnated calcium phosphate filler for enhanced bone regeneration	1007:1162	On the basis of the desirable results obtained, we provide a preliminary rationale for the use of methanolic extract-blended chitosan microparticle-impregnated calcium phosphate filler for enhanced bone regeneration.
32272467	1	25	theme	Withania	123:130	arg1	ashwagandha					143:153	ashwagandha	143:153	ashwagandha	143:153	Withania somnifera (ashwagandha) is used in Indian traditional medicine for its various health benefits.
32272467	1	25	theme	Withania	123:130	arg1	somnifera					132:140	Withania somnifera	123:140	Withania somnifera (ashwagandha)	123:154	Withania somnifera (ashwagandha) is used in Indian traditional medicine for its various health benefits.
32272467	1	26	theme	health	211:216	arg1	benefits					218:225	its various health benefits	199:225	its various health benefits	199:225	Withania somnifera (ashwagandha) is used in Indian traditional medicine for its various health benefits.
32272467	4	27	theme	enhanced	603:610	arg1	proliferation					612:624	proliferation	612:624	proliferation	612:624	The controlled release of bioactive molecules enabled enhanced proliferation and differentiation of pre-osteoblasts.
32272467	5	28	theme	Microparticle	666:678	arg1	percentages					680:690	Microparticle percentages	666:690	Microparticle percentages	666:690	Microparticle percentages were optimized to have a minimum effect on the setting time, mechanical strength and degradability of hydroxyapatite bone filler.
32272467	2	29	theme	bone	338:341	arg1	mineralization					343:356	bone mineralization	338:356	bone mineralization	338:356	Withaferin-A, a steroidal lactone present in this herb, has shown proteosomal inhibition-based enhancement of bone mineralization.
32272467	3	30	theme	calcium	497:503	arg1	filler					541:546	calcium phosphate-based hydroxyapatite bone filler	497:546	calcium phosphate-based hydroxyapatite bone filler	497:546	In the present work, chitosan microparticles blended with total methanolic root extract of W. somnifera were incorporated as a porogen in calcium phosphate-based hydroxyapatite bone filler.
32272467	0	31	theme	ceramic	56:62	arg1	bone					64:67	hydroxyapatite-based ceramic bone	35:67	hydroxyapatite-based ceramic bone	35:67	Enhanced bone mineralization using hydroxyapatite-based ceramic bone substitute incorporating Withania somnifera extracts.
32272467	5	32	theme	hydroxyapatite	794:807	arg1	filler					814:819	hydroxyapatite bone filler	794:819	hydroxyapatite bone filler	794:819	Microparticle percentages were optimized to have a minimum effect on the setting time, mechanical strength and degradability of hydroxyapatite bone filler.
32272467	2	33	theme	present	262:268	arg1	lactone					254:260	a steroidal lactone	242:260	a steroidal lactone present in this herb	242:281	Withaferin-A, a steroidal lactone present in this herb, has shown proteosomal inhibition-based enhancement of bone mineralization.
32272467	2	33	theme	present	262:268	arg1	Withaferin-A					228:239	Withaferin-A	228:239	Withaferin-A	228:239	Withaferin-A, a steroidal lactone present in this herb, has shown proteosomal inhibition-based enhancement of bone mineralization.
32272467	2	34	from	herb	278:281	arg1	present					262:268	present	262:268	present	262:268	Withaferin-A, a steroidal lactone present in this herb, has shown proteosomal inhibition-based enhancement of bone mineralization.
32272467	0	35	theme	hydroxyapatite-based	35:54	arg1	bone					64:67	hydroxyapatite-based ceramic bone	35:67	hydroxyapatite-based ceramic bone	35:67	Enhanced bone mineralization using hydroxyapatite-based ceramic bone substitute incorporating Withania somnifera extracts.
32272467	4	36	theme	bioactive	575:583	arg1	molecules					585:593	bioactive molecules	575:593	bioactive molecules	575:593	The controlled release of bioactive molecules enabled enhanced proliferation and differentiation of pre-osteoblasts.
32272467	5	37	theme	bone	809:812	arg1	filler					814:819	hydroxyapatite bone filler	794:819	hydroxyapatite bone filler	794:819	Microparticle percentages were optimized to have a minimum effect on the setting time, mechanical strength and degradability of hydroxyapatite bone filler.
32272467	7	38	theme	enhanced	1137:1144	arg1	regeneration					1151:1162	enhanced bone regeneration	1137:1162	enhanced bone regeneration	1137:1162	On the basis of the desirable results obtained, we provide a preliminary rationale for the use of methanolic extract-blended chitosan microparticle-impregnated calcium phosphate filler for enhanced bone regeneration.
32272467	7	39	theme	calcium	1108:1114	arg1	filler					1126:1131	methanolic extract-blended chitosan microparticle-impregnated calcium phosphate filler	1046:1131	methanolic extract-blended chitosan microparticle-impregnated calcium phosphate filler	1046:1131	On the basis of the desirable results obtained, we provide a preliminary rationale for the use of methanolic extract-blended chitosan microparticle-impregnated calcium phosphate filler for enhanced bone regeneration.
32272467	3	40	theme	root	434:437	arg1	extract					439:445	total methanolic root extract	417:445	total methanolic root extract of W. somnifera	417:461	In the present work, chitosan microparticles blended with total methanolic root extract of W. somnifera were incorporated as a porogen in calcium phosphate-based hydroxyapatite bone filler.
32272467	5	41	theme	filler	814:819	arg1	strength					764:771	mechanical strength	753:771	mechanical strength	753:771	Microparticle percentages were optimized to have a minimum effect on the setting time, mechanical strength and degradability of hydroxyapatite bone filler.
32272467	5	41	theme	filler	814:819	arg1	degradability					777:789	degradability	777:789	degradability	777:789	Microparticle percentages were optimized to have a minimum effect on the setting time, mechanical strength and degradability of hydroxyapatite bone filler.
32272467	5	41	theme	filler	814:819	arg1	time					747:750	the setting time	735:750	the setting time	735:750	Microparticle percentages were optimized to have a minimum effect on the setting time, mechanical strength and degradability of hydroxyapatite bone filler.
32272467	2	42	theme	steroidal	244:252	arg1	lactone					254:260	a steroidal lactone	242:260	a steroidal lactone present in this herb	242:281	Withaferin-A, a steroidal lactone present in this herb, has shown proteosomal inhibition-based enhancement of bone mineralization.
32272467	2	42	theme	steroidal	244:252	arg1	Withaferin-A					228:239	Withaferin-A	228:239	Withaferin-A	228:239	Withaferin-A, a steroidal lactone present in this herb, has shown proteosomal inhibition-based enhancement of bone mineralization.
32272467	3	43	from	porogen	486:492	arg1	filler					541:546	calcium phosphate-based hydroxyapatite bone filler	497:546	calcium phosphate-based hydroxyapatite bone filler	497:546	In the present work, chitosan microparticles blended with total methanolic root extract of W. somnifera were incorporated as a porogen in calcium phosphate-based hydroxyapatite bone filler.
32272467	3	44	theme	bone	536:539	arg1	filler					541:546	calcium phosphate-based hydroxyapatite bone filler	497:546	calcium phosphate-based hydroxyapatite bone filler	497:546	In the present work, chitosan microparticles blended with total methanolic root extract of W. somnifera were incorporated as a porogen in calcium phosphate-based hydroxyapatite bone filler.
32272467	5	45	theme	mechanical	753:762	arg1	strength					764:771	mechanical strength	753:771	mechanical strength	753:771	Microparticle percentages were optimized to have a minimum effect on the setting time, mechanical strength and degradability of hydroxyapatite bone filler.
32272467	7	46	theme	filler	1126:1131	arg1	use					1039:1041	the use	1035:1041	the use of methanolic extract-blended chitosan microparticle-impregnated calcium phosphate filler	1035:1131	On the basis of the desirable results obtained, we provide a preliminary rationale for the use of methanolic extract-blended chitosan microparticle-impregnated calcium phosphate filler for enhanced bone regeneration.
32272467	4	47	theme	molecules	585:593	arg1	release					564:570	The controlled release	549:570	The controlled release of bioactive molecules	549:593	The controlled release of bioactive molecules enabled enhanced proliferation and differentiation of pre-osteoblasts.
32272467	6	48	theme	composites	936:945	arg1	biocompatibility					912:927	the biocompatibility	908:927	the biocompatibility of the composites	908:945	In vitro cell adhesion, proliferation and differentiation were evaluated to determine the biocompatibility of the composites.
32272467	6	49	theme	cell	831:834	arg1	adhesion					836:843	vitro cell adhesion	825:843	vitro cell adhesion	825:843	In vitro cell adhesion, proliferation and differentiation were evaluated to determine the biocompatibility of the composites.
32272467	3	50	theme	phosphate-based	505:519	arg1	filler					541:546	calcium phosphate-based hydroxyapatite bone filler	497:546	calcium phosphate-based hydroxyapatite bone filler	497:546	In the present work, chitosan microparticles blended with total methanolic root extract of W. somnifera were incorporated as a porogen in calcium phosphate-based hydroxyapatite bone filler.
32272467	6	51	theme	vitro	825:829	arg1	adhesion					836:843	vitro cell adhesion	825:843	vitro cell adhesion	825:843	In vitro cell adhesion, proliferation and differentiation were evaluated to determine the biocompatibility of the composites.
32272467	3	52	theme	hydroxyapatite	521:534	arg1	filler					541:546	calcium phosphate-based hydroxyapatite bone filler	497:546	calcium phosphate-based hydroxyapatite bone filler	497:546	In the present work, chitosan microparticles blended with total methanolic root extract of W. somnifera were incorporated as a porogen in calcium phosphate-based hydroxyapatite bone filler.
32272467	0	53	theme	Withania	94:101	arg1	extracts					113:120	Withania somnifera extracts	94:120	Withania somnifera extracts	94:120	Enhanced bone mineralization using hydroxyapatite-based ceramic bone substitute incorporating Withania somnifera extracts.
32272467	7	54	theme	phosphate	1116:1124	arg1	filler					1126:1131	methanolic extract-blended chitosan microparticle-impregnated calcium phosphate filler	1046:1131	methanolic extract-blended chitosan microparticle-impregnated calcium phosphate filler	1046:1131	On the basis of the desirable results obtained, we provide a preliminary rationale for the use of methanolic extract-blended chitosan microparticle-impregnated calcium phosphate filler for enhanced bone regeneration.
32272467	3	55	theme	total	417:421	arg1	extract					439:445	total methanolic root extract	417:445	total methanolic root extract of W. somnifera	417:461	In the present work, chitosan microparticles blended with total methanolic root extract of W. somnifera were incorporated as a porogen in calcium phosphate-based hydroxyapatite bone filler.
32272467	3	56	theme	present	366:372	arg1	work					374:377	the present work	362:377	the present work	362:377	In the present work, chitosan microparticles blended with total methanolic root extract of W. somnifera were incorporated as a porogen in calcium phosphate-based hydroxyapatite bone filler.
32272467	7	57	theme	chitosan	1073:1080	arg1	filler					1126:1131	methanolic extract-blended chitosan microparticle-impregnated calcium phosphate filler	1046:1131	methanolic extract-blended chitosan microparticle-impregnated calcium phosphate filler	1046:1131	On the basis of the desirable results obtained, we provide a preliminary rationale for the use of methanolic extract-blended chitosan microparticle-impregnated calcium phosphate filler for enhanced bone regeneration.
32272467	1	58	theme	Indian	167:172	arg1	medicine					186:193	Indian traditional medicine	167:193	Indian traditional medicine	167:193	Withania somnifera (ashwagandha) is used in Indian traditional medicine for its various health benefits.
32272467	7	59	dep	results	978:984	arg1	the					951:953	the	951:953	the	951:953	On the basis of the desirable results obtained, we provide a preliminary rationale for the use of methanolic extract-blended chitosan microparticle-impregnated calcium phosphate filler for enhanced bone regeneration.
32272467	7	59	dep	results	978:984	arg1	basis					955:959	basis	955:959	basis	955:959	On the basis of the desirable results obtained, we provide a preliminary rationale for the use of methanolic extract-blended chitosan microparticle-impregnated calcium phosphate filler for enhanced bone regeneration.
32821668	6	0	theme	milking	973:979	arg1	procedure					981:989	the milking procedure	969:989	the milking procedure	969:989	Afterward, the calves were used to stimulate milk letdown for each of the camels' teats for a few seconds before the milking procedure.
32821668	7	1	theme	late	1005:1008	arg1	G2					1025:1026	G2	1025:1026	G2	1025:1026	While in the late weaning group (G2), the calves freely suckled their mother's teats up to 80 days postpartum.
32821668	7	1	theme	late	1005:1008	arg1	group					1018:1022	the late weaning group	1001:1022	the late weaning group (G2)	1001:1027	While in the late weaning group (G2), the calves freely suckled their mother's teats up to 80 days postpartum.
32821668	10	2	theme	density	1509:1515	arg1	content					1517:1523	density content	1509:1523	density content	1509:1523	Results The results revealed significant differences in fat, lactose, and density content between groups.
32821668	3	3	theme	modern	503:508	arg1	system					518:523	a modern farming system	501:523	a modern farming system	501:523	Aim This study aims to clarify the impact of early weaning on constituents and nutritional values of the milk of camels reared under a modern farming system during four months postpartum.
32821668	12	4	theme	high	1786:1789	arg1	levels					1791:1796	high levels	1786:1796	high levels of fat and SNF	1786:1811	Conclusion In the early weaning group, the dams produced milk containing high levels of fat and SNF compared to the lactating dams in the late weaning group.
32821668	9	5	theme	postpartum	1283:1292	arg1	week					1275:1278	the 2nd week	1267:1278	the 2nd week of postpartum	1267:1292	Collection of milk samples started in the 2nd week of postpartum and continued over biweekly intervals up to 16 weeks to determine major fat, protein, lactose, solid non-fat (SNF), and density percentages.
32821668	5	6	from	birth	827:831	arg1	teats					816:820	their mother's teats	801:820	their mother's teats from birth to 30 days postpartum	801:853	In the early weaning group (G1), all the calves were allowed to freely suckle their mother's teats from birth to 30 days postpartum.
32821668	11	7	from	increases	1565:1573	arg1	percentages					1582:1592	the percentages	1578:1592	the percentages	1578:1592	We reported significant increases in the percentages of fat, lactose, protein, and SNF levels, besides fluctuations in density with the advancement of the lactation stage.
32821668	9	8	theme	2nd	1271:1273	arg1	week					1275:1278	the 2nd week	1267:1278	the 2nd week of postpartum	1267:1292	Collection of milk samples started in the 2nd week of postpartum and continued over biweekly intervals up to 16 weeks to determine major fat, protein, lactose, solid non-fat (SNF), and density percentages.
32821668	12	9	theme	weaning	1856:1862	arg1	group					1864:1868	the late weaning group	1847:1868	the late weaning group	1847:1868	Conclusion In the early weaning group, the dams produced milk containing high levels of fat and SNF compared to the lactating dams in the late weaning group.
32821668	4	10	dep	Methods	556:562	arg1	camels					580:585	Eight lactating camels	564:585	Eight lactating camels	564:585	Methods Eight lactating camels and eight calves were selected immediately after calving and were assigned to two equal groups under a semi-intensive system in a farm.
32821668	4	10	dep	Methods	556:562	arg1	calves					597:602	eight calves	591:602	eight calves	591:602	Methods Eight lactating camels and eight calves were selected immediately after calving and were assigned to two equal groups under a semi-intensive system in a farm.
32821668	3	11	theme	milk	473:476	arg1	values					459:464	nutritional values	447:464	nutritional values	447:464	Aim This study aims to clarify the impact of early weaning on constituents and nutritional values of the milk of camels reared under a modern farming system during four months postpartum.
32821668	3	11	theme	milk	473:476	arg1	constituents					430:441	constituents	430:441	constituents	430:441	Aim This study aims to clarify the impact of early weaning on constituents and nutritional values of the milk of camels reared under a modern farming system during four months postpartum.
32821668	12	12	theme	lactating	1829:1837	arg1	dams					1839:1842	the lactating dams	1825:1842	the lactating dams	1825:1842	Conclusion In the early weaning group, the dams produced milk containing high levels of fat and SNF compared to the lactating dams in the late weaning group.
32821668	9	13	theme	biweekly	1313:1320	arg1	intervals					1322:1330	biweekly intervals	1313:1330	biweekly intervals up to 16 weeks to determine major fat, protein, lactose, solid non-fat (SNF), and density percentages	1313:1432	Collection of milk samples started in the 2nd week of postpartum and continued over biweekly intervals up to 16 weeks to determine major fat, protein, lactose, solid non-fat (SNF), and density percentages.
32821668	12	14	theme	weaning	1737:1743	arg1	group					1745:1749	the early weaning group	1727:1749	the early weaning group	1727:1749	Conclusion In the early weaning group, the dams produced milk containing high levels of fat and SNF compared to the lactating dams in the late weaning group.
32821668	0	15	from	values	56:61	arg1	system					87:92	modern system	80:92	modern system	80:92	Impact of early weaning on constituents and nutritional values of camel milk in modern system.
32821668	1	16	theme	Camel	106:110	arg1	milk					112:115	Background Camel milk	95:115	Background Camel milk	95:115	Background Camel milk is very suitable for human nutritional requirements, and its composition has similarities to mother's milk.
32821668	9	17	theme	solid	1389:1393	arg1	SNF					1404:1406	SNF	1404:1406	SNF	1404:1406	Collection of milk samples started in the 2nd week of postpartum and continued over biweekly intervals up to 16 weeks to determine major fat, protein, lactose, solid non-fat (SNF), and density percentages.
32821668	9	17	theme	solid	1389:1393	arg1	non-fat					1395:1401	solid non-fat	1389:1401	solid non-fat (SNF)	1389:1407	Collection of milk samples started in the 2nd week of postpartum and continued over biweekly intervals up to 16 weeks to determine major fat, protein, lactose, solid non-fat (SNF), and density percentages.
32821668	6	18	used	used	883:886	arg2	calves					871:876	the calves	867:876	the calves	867:876	Afterward, the calves were used to stimulate milk letdown for each of the camels' teats for a few seconds before the milking procedure.
32821668	2	19	theme	scientific	230:239	arg1	researches					241:250	Many scientific researches	225:250	Many scientific researches focusing on the myth of nutritional and therapeutic properties of camel milk	225:327	Many scientific researches focusing on the myth of nutritional and therapeutic properties of camel milk have been brought to public attention.
32821668	5	20	theme	days	839:842	arg1	postpartum					844:853	30 days postpartum	836:853	30 days postpartum	836:853	In the early weaning group (G1), all the calves were allowed to freely suckle their mother's teats from birth to 30 days postpartum.
32821668	0	21	theme	camel	66:70	arg1	milk					72:75	camel milk	66:75	camel milk	66:75	Impact of early weaning on constituents and nutritional values of camel milk in modern system.
32821668	8	22	from	suckling	1146:1153	arg1	restricted					1130:1139	restricted	1130:1139	restricted	1130:1139	Afterward, the calves were restricted from suckling and were used mainly to stimulate milk letdown for the milking procedure.
32821668	8	22	from	suckling	1146:1153	arg1	calves					1118:1123	the calves	1114:1123	the calves	1114:1123	Afterward, the calves were restricted from suckling and were used mainly to stimulate milk letdown for the milking procedure.
32821668	1	23	theme	human	138:142	arg1	requirements					156:167	human nutritional requirements	138:167	human nutritional requirements	138:167	Background Camel milk is very suitable for human nutritional requirements, and its composition has similarities to mother's milk.
32821668	6	24	theme	milk	901:904	arg1	letdown					906:912	milk letdown	901:912	milk letdown for each of the camels' teats for a few seconds before the milking procedure	901:989	Afterward, the calves were used to stimulate milk letdown for each of the camels' teats for a few seconds before the milking procedure.
32821668	0	25	from	Impact	0:5	arg1	constituents					27:38	constituents	27:38	constituents	27:38	Impact of early weaning on constituents and nutritional values of camel milk in modern system.
32821668	0	25	from	Impact	0:5	arg1	values					56:61	nutritional values	44:61	nutritional values of camel milk in modern system	44:92	Impact of early weaning on constituents and nutritional values of camel milk in modern system.
32821668	2	26	theme	properties	304:313	arg1	myth					268:271	the myth	264:271	the myth of nutritional and therapeutic properties of camel milk	264:327	Many scientific researches focusing on the myth of nutritional and therapeutic properties of camel milk have been brought to public attention.
32821668	11	27	theme	stage	1706:1710	arg1	advancement					1677:1687	the advancement	1673:1687	the advancement of the lactation stage	1673:1710	We reported significant increases in the percentages of fat, lactose, protein, and SNF levels, besides fluctuations in density with the advancement of the lactation stage.
32821668	4	28	from	system	705:710	arg1	farm					717:720	a farm	715:720	a farm	715:720	Methods Eight lactating camels and eight calves were selected immediately after calving and were assigned to two equal groups under a semi-intensive system in a farm.
32821668	9	29	dep	weeks	1341:1345	arg1	up					1332:1333	up	1332:1333	up	1332:1333	Collection of milk samples started in the 2nd week of postpartum and continued over biweekly intervals up to 16 weeks to determine major fat, protein, lactose, solid non-fat (SNF), and density percentages.
32821668	0	30	theme	early	10:14	arg1	weaning					16:22	early weaning	10:22	early weaning	10:22	Impact of early weaning on constituents and nutritional values of camel milk in modern system.
32821668	9	31	theme	non-fat	1395:1401	arg1	percentages					1422:1432	major fat, protein, lactose, solid non-fat (SNF), and density percentages	1360:1432	major fat, protein, lactose, solid non-fat (SNF), and density percentages	1360:1432	Collection of milk samples started in the 2nd week of postpartum and continued over biweekly intervals up to 16 weeks to determine major fat, protein, lactose, solid non-fat (SNF), and density percentages.
32821668	3	32	theme	early	413:417	arg1	weaning					419:425	early weaning	413:425	early weaning	413:425	Aim This study aims to clarify the impact of early weaning on constituents and nutritional values of the milk of camels reared under a modern farming system during four months postpartum.
32821668	11	33	theme	lactation	1696:1704	arg1	stage					1706:1710	the lactation stage	1692:1710	the lactation stage	1692:1710	We reported significant increases in the percentages of fat, lactose, protein, and SNF levels, besides fluctuations in density with the advancement of the lactation stage.
32821668	5	34	theme	weaning	736:742	arg1	group					744:748	the early weaning group	726:748	the early weaning group (G1)	726:753	In the early weaning group (G1), all the calves were allowed to freely suckle their mother's teats from birth to 30 days postpartum.
32821668	5	34	theme	weaning	736:742	arg1	G1					751:752	G1	751:752	G1	751:752	In the early weaning group (G1), all the calves were allowed to freely suckle their mother's teats from birth to 30 days postpartum.
32821668	7	35	dep	freely	1041:1046	arg1	suckled					1048:1054	suckled	1048:1054	freely suckled their mother's teats up to 80 days postpartum	1041:1100	While in the late weaning group (G2), the calves freely suckled their mother's teats up to 80 days postpartum.
32821668	2	36	theme	milk	324:327	arg1	properties					304:313	nutritional and therapeutic properties	276:313	nutritional and therapeutic properties of camel milk	276:327	Many scientific researches focusing on the myth of nutritional and therapeutic properties of camel milk have been brought to public attention.
32821668	1	37	contain	has	190:192	arg2	similarities					194:205	similarities	194:205	similarities to mother's milk	194:222	Background Camel milk is very suitable for human nutritional requirements, and its composition has similarities to mother's milk.
32821668	1	37	contain	has	190:192	arg1	composition					178:188	its composition	174:188	its composition	174:188	Background Camel milk is very suitable for human nutritional requirements, and its composition has similarities to mother's milk.
32821668	11	38	theme	SNF	1624:1626	arg1	levels					1628:1633	SNF levels	1624:1633	SNF levels	1624:1633	We reported significant increases in the percentages of fat, lactose, protein, and SNF levels, besides fluctuations in density with the advancement of the lactation stage.
32821668	9	39	theme	density	1414:1420	arg1	percentages					1422:1432	major fat, protein, lactose, solid non-fat (SNF), and density percentages	1360:1432	major fat, protein, lactose, solid non-fat (SNF), and density percentages	1360:1432	Collection of milk samples started in the 2nd week of postpartum and continued over biweekly intervals up to 16 weeks to determine major fat, protein, lactose, solid non-fat (SNF), and density percentages.
32821668	10	40	from	differences	1476:1486	arg1	content					1517:1523	density content	1509:1523	density content	1509:1523	Results The results revealed significant differences in fat, lactose, and density content between groups.
32821668	10	40	from	differences	1476:1486	arg1	lactose					1496:1502	lactose	1496:1502	lactose	1496:1502	Results The results revealed significant differences in fat, lactose, and density content between groups.
32821668	10	40	from	differences	1476:1486	arg1	fat					1491:1493	fat	1491:1493	fat	1491:1493	Results The results revealed significant differences in fat, lactose, and density content between groups.
32821668	3	41	theme	weaning	419:425	arg1	impact					403:408	the impact	399:408	the impact of early weaning on constituents and nutritional values of the milk of camels reared under a modern farming system during four months postpartum	399:553	Aim This study aims to clarify the impact of early weaning on constituents and nutritional values of the milk of camels reared under a modern farming system during four months postpartum.
32821668	7	42	theme	weaning	1010:1016	arg1	G2					1025:1026	G2	1025:1026	G2	1025:1026	While in the late weaning group (G2), the calves freely suckled their mother's teats up to 80 days postpartum.
32821668	7	42	theme	weaning	1010:1016	arg1	group					1018:1022	the late weaning group	1001:1022	the late weaning group (G2)	1001:1027	While in the late weaning group (G2), the calves freely suckled their mother's teats up to 80 days postpartum.
32821668	9	43	theme	samples	1248:1254	arg1	Collection					1229:1238	Collection	1229:1238	Collection of milk samples	1229:1254	Collection of milk samples started in the 2nd week of postpartum and continued over biweekly intervals up to 16 weeks to determine major fat, protein, lactose, solid non-fat (SNF), and density percentages.
32821668	12	44	theme	fat	1801:1803	arg1	levels					1791:1796	high levels	1786:1796	high levels of fat and SNF	1786:1811	Conclusion In the early weaning group, the dams produced milk containing high levels of fat and SNF compared to the lactating dams in the late weaning group.
32821668	7	45	theme	days	1086:1089	arg1	postpartum					1091:1100	80 days postpartum	1083:1100	80 days postpartum	1083:1100	While in the late weaning group (G2), the calves freely suckled their mother's teats up to 80 days postpartum.
32821668	11	46	with	density	1660:1666	arg1	advancement					1677:1687	the advancement	1673:1687	the advancement of the lactation stage	1673:1710	We reported significant increases in the percentages of fat, lactose, protein, and SNF levels, besides fluctuations in density with the advancement of the lactation stage.
32821668	12	47	contain	containing	1775:1784	arg1	milk					1770:1773	milk	1770:1773	milk containing high levels of fat and SNF	1770:1811	Conclusion In the early weaning group, the dams produced milk containing high levels of fat and SNF compared to the lactating dams in the late weaning group.
32821668	12	47	contain	containing	1775:1784	arg2	levels					1791:1796	high levels	1786:1796	high levels of fat and SNF	1786:1811	Conclusion In the early weaning group, the dams produced milk containing high levels of fat and SNF compared to the lactating dams in the late weaning group.
32821668	1	48	theme	nutritional	144:154	arg1	requirements					156:167	human nutritional requirements	138:167	human nutritional requirements	138:167	Background Camel milk is very suitable for human nutritional requirements, and its composition has similarities to mother's milk.
32821668	2	49	theme	therapeutic	292:302	arg1	properties					304:313	nutritional and therapeutic properties	276:313	nutritional and therapeutic properties of camel milk	276:327	Many scientific researches focusing on the myth of nutritional and therapeutic properties of camel milk have been brought to public attention.
32821668	11	50	theme	lactose	1602:1608	arg1	percentages					1582:1592	the percentages	1578:1592	the percentages	1578:1592	We reported significant increases in the percentages of fat, lactose, protein, and SNF levels, besides fluctuations in density with the advancement of the lactation stage.
32821668	2	51	theme	nutritional	276:286	arg1	properties					304:313	nutritional and therapeutic properties	276:313	nutritional and therapeutic properties of camel milk	276:327	Many scientific researches focusing on the myth of nutritional and therapeutic properties of camel milk have been brought to public attention.
32821668	9	52	theme	major	1360:1364	arg1	percentages					1422:1432	major fat, protein, lactose, solid non-fat (SNF), and density percentages	1360:1432	major fat, protein, lactose, solid non-fat (SNF), and density percentages	1360:1432	Collection of milk samples started in the 2nd week of postpartum and continued over biweekly intervals up to 16 weeks to determine major fat, protein, lactose, solid non-fat (SNF), and density percentages.
32821668	12	53	theme	late	1851:1854	arg1	group					1864:1868	the late weaning group	1847:1868	the late weaning group	1847:1868	Conclusion In the early weaning group, the dams produced milk containing high levels of fat and SNF compared to the lactating dams in the late weaning group.
32821668	12	54	dep	Conclusion	1713:1722	arg1	group					1745:1749	the early weaning group	1727:1749	the early weaning group	1727:1749	Conclusion In the early weaning group, the dams produced milk containing high levels of fat and SNF compared to the lactating dams in the late weaning group.
32821668	11	55	theme	fat	1597:1599	arg1	percentages					1582:1592	the percentages	1578:1592	the percentages	1578:1592	We reported significant increases in the percentages of fat, lactose, protein, and SNF levels, besides fluctuations in density with the advancement of the lactation stage.
32821668	3	56	theme	months	537:542	arg1	postpartum					544:553	four months postpartum	532:553	four months postpartum	532:553	Aim This study aims to clarify the impact of early weaning on constituents and nutritional values of the milk of camels reared under a modern farming system during four months postpartum.
32821668	1	57	theme	Background	95:104	arg1	milk					112:115	Background Camel milk	95:115	Background Camel milk	95:115	Background Camel milk is very suitable for human nutritional requirements, and its composition has similarities to mother's milk.
32821668	12	58	theme	early	1731:1735	arg1	group					1745:1749	the early weaning group	1727:1749	the early weaning group	1727:1749	Conclusion In the early weaning group, the dams produced milk containing high levels of fat and SNF compared to the lactating dams in the late weaning group.
32821668	4	59	theme	equal	669:673	arg1	groups					675:680	two equal groups	665:680	two equal groups under a semi-intensive system in a farm	665:720	Methods Eight lactating camels and eight calves were selected immediately after calving and were assigned to two equal groups under a semi-intensive system in a farm.
32821668	11	60	theme	protein	1611:1617	arg1	percentages					1582:1592	the percentages	1578:1592	the percentages	1578:1592	We reported significant increases in the percentages of fat, lactose, protein, and SNF levels, besides fluctuations in density with the advancement of the lactation stage.
32821668	8	61	used	used	1164:1167	arg2	calves					1118:1123	the calves	1114:1123	the calves	1114:1123	Afterward, the calves were restricted from suckling and were used mainly to stimulate milk letdown for the milking procedure.
32821668	8	61	used	used	1164:1167	arg2	restricted					1130:1139	restricted	1130:1139	restricted	1130:1139	Afterward, the calves were restricted from suckling and were used mainly to stimulate milk letdown for the milking procedure.
32821668	3	62	theme	farming	510:516	arg1	system					518:523	a modern farming system	501:523	a modern farming system	501:523	Aim This study aims to clarify the impact of early weaning on constituents and nutritional values of the milk of camels reared under a modern farming system during four months postpartum.
32821668	11	63	theme	significant	1553:1563	arg1	increases					1565:1573	significant increases	1553:1573	significant increases in the percentages of fat, lactose, protein, and SNF levels, besides fluctuations in density with the advancement of the lactation stage	1553:1710	We reported significant increases in the percentages of fat, lactose, protein, and SNF levels, besides fluctuations in density with the advancement of the lactation stage.
32821668	2	64	theme	Many	225:228	arg1	researches					241:250	Many scientific researches	225:250	Many scientific researches focusing on the myth of nutritional and therapeutic properties of camel milk	225:327	Many scientific researches focusing on the myth of nutritional and therapeutic properties of camel milk have been brought to public attention.
32821668	12	65	theme	SNF	1809:1811	arg1	levels					1791:1796	high levels	1786:1796	high levels of fat and SNF	1786:1811	Conclusion In the early weaning group, the dams produced milk containing high levels of fat and SNF compared to the lactating dams in the late weaning group.
32821668	0	66	theme	milk	72:75	arg1	constituents					27:38	constituents	27:38	constituents	27:38	Impact of early weaning on constituents and nutritional values of camel milk in modern system.
32821668	0	66	theme	milk	72:75	arg1	values					56:61	nutritional values	44:61	nutritional values of camel milk in modern system	44:92	Impact of early weaning on constituents and nutritional values of camel milk in modern system.
32821668	11	67	from	fluctuations	1644:1655	arg1	density					1660:1666	density	1660:1666	density with the advancement of the lactation stage	1660:1710	We reported significant increases in the percentages of fat, lactose, protein, and SNF levels, besides fluctuations in density with the advancement of the lactation stage.
32821668	0	68	theme	modern	80:85	arg1	system					87:92	modern system	80:92	modern system	80:92	Impact of early weaning on constituents and nutritional values of camel milk in modern system.
32821668	9	69	theme	fat	1366:1368	arg1	percentages					1422:1432	major fat, protein, lactose, solid non-fat (SNF), and density percentages	1360:1432	major fat, protein, lactose, solid non-fat (SNF), and density percentages	1360:1432	Collection of milk samples started in the 2nd week of postpartum and continued over biweekly intervals up to 16 weeks to determine major fat, protein, lactose, solid non-fat (SNF), and density percentages.
32821668	3	70	dep	Aim	368:370	arg1	aims					383:386	aims	383:386	aims to clarify the impact of early weaning on constituents and nutritional values of the milk of camels reared under a modern farming system during four months postpartum	383:553	Aim This study aims to clarify the impact of early weaning on constituents and nutritional values of the milk of camels reared under a modern farming system during four months postpartum.
32821668	11	71	theme	levels	1628:1633	arg1	percentages					1582:1592	the percentages	1578:1592	the percentages	1578:1592	We reported significant increases in the percentages of fat, lactose, protein, and SNF levels, besides fluctuations in density with the advancement of the lactation stage.
32821668	9	72	theme	protein	1371:1377	arg1	percentages					1422:1432	major fat, protein, lactose, solid non-fat (SNF), and density percentages	1360:1432	major fat, protein, lactose, solid non-fat (SNF), and density percentages	1360:1432	Collection of milk samples started in the 2nd week of postpartum and continued over biweekly intervals up to 16 weeks to determine major fat, protein, lactose, solid non-fat (SNF), and density percentages.
32821668	0	73	from	constituents	27:38	arg1	system					87:92	modern system	80:92	modern system	80:92	Impact of early weaning on constituents and nutritional values of camel milk in modern system.
32821668	8	74	theme	milk	1189:1192	arg1	letdown					1194:1200	milk letdown	1189:1200	milk letdown for the milking procedure	1189:1226	Afterward, the calves were restricted from suckling and were used mainly to stimulate milk letdown for the milking procedure.
32821668	9	75	theme	lactose	1380:1386	arg1	percentages					1422:1432	major fat, protein, lactose, solid non-fat (SNF), and density percentages	1360:1432	major fat, protein, lactose, solid non-fat (SNF), and density percentages	1360:1432	Collection of milk samples started in the 2nd week of postpartum and continued over biweekly intervals up to 16 weeks to determine major fat, protein, lactose, solid non-fat (SNF), and density percentages.
32821668	6	76	theme	few	950:952	arg1	seconds					954:960	a few seconds	948:960	a few seconds before the milking procedure	948:989	Afterward, the calves were used to stimulate milk letdown for each of the camels' teats for a few seconds before the milking procedure.
32821668	4	77	theme	semi-intensive	690:703	arg1	system					705:710	a semi-intensive system	688:710	a semi-intensive system in a farm	688:720	Methods Eight lactating camels and eight calves were selected immediately after calving and were assigned to two equal groups under a semi-intensive system in a farm.
32821668	2	78	theme	public	350:355	arg1	attention					357:365	public attention	350:365	public attention	350:365	Many scientific researches focusing on the myth of nutritional and therapeutic properties of camel milk have been brought to public attention.
32821668	0	79	theme	weaning	16:22	arg1	Impact					0:5	Impact	0:5	Impact of early weaning on constituents and nutritional values of camel milk in modern system.	0:93	Impact of early weaning on constituents and nutritional values of camel milk in modern system.
32821668	7	80	dep	postpartum	1091:1100	arg1	up					1077:1078	up	1077:1078	up	1077:1078	While in the late weaning group (G2), the calves freely suckled their mother's teats up to 80 days postpartum.
32821668	8	81	theme	milking	1210:1216	arg1	procedure					1218:1226	the milking procedure	1206:1226	the milking procedure	1206:1226	Afterward, the calves were restricted from suckling and were used mainly to stimulate milk letdown for the milking procedure.
32821668	5	82	theme	early	730:734	arg1	group					744:748	the early weaning group	726:748	the early weaning group (G1)	726:753	In the early weaning group (G1), all the calves were allowed to freely suckle their mother's teats from birth to 30 days postpartum.
32821668	5	82	theme	early	730:734	arg1	G1					751:752	G1	751:752	G1	751:752	In the early weaning group (G1), all the calves were allowed to freely suckle their mother's teats from birth to 30 days postpartum.
32821668	10	83	theme	significant	1464:1474	arg1	differences					1476:1486	significant differences	1464:1486	significant differences in fat, lactose, and density content between groups	1464:1538	Results The results revealed significant differences in fat, lactose, and density content between groups.
32821668	0	84	theme	nutritional	44:54	arg1	values					56:61	nutritional values	44:61	nutritional values of camel milk in modern system	44:92	Impact of early weaning on constituents and nutritional values of camel milk in modern system.
32821668	2	85	theme	camel	318:322	arg1	milk					324:327	camel milk	318:327	camel milk	318:327	Many scientific researches focusing on the myth of nutritional and therapeutic properties of camel milk have been brought to public attention.
32821668	4	86	theme	lactating	570:578	arg1	camels					580:585	Eight lactating camels	564:585	Eight lactating camels	564:585	Methods Eight lactating camels and eight calves were selected immediately after calving and were assigned to two equal groups under a semi-intensive system in a farm.
32821668	10	87	dep	Results	1435:1441	arg1	results					1447:1453	The results	1443:1453	Results The results	1435:1453	Results The results revealed significant differences in fat, lactose, and density content between groups.
32821668	3	88	theme	nutritional	447:457	arg1	values					459:464	nutritional values	447:464	nutritional values	447:464	Aim This study aims to clarify the impact of early weaning on constituents and nutritional values of the milk of camels reared under a modern farming system during four months postpartum.
32821668	3	89	from	impact	403:408	arg1	values					459:464	nutritional values	447:464	nutritional values	447:464	Aim This study aims to clarify the impact of early weaning on constituents and nutritional values of the milk of camels reared under a modern farming system during four months postpartum.
32821668	3	89	from	impact	403:408	arg1	constituents					430:441	constituents	430:441	constituents	430:441	Aim This study aims to clarify the impact of early weaning on constituents and nutritional values of the milk of camels reared under a modern farming system during four months postpartum.
32821668	3	90	theme	camels	481:486	arg1	milk					473:476	the milk	469:476	the milk of camels reared under a modern farming system during four months postpartum	469:553	Aim This study aims to clarify the impact of early weaning on constituents and nutritional values of the milk of camels reared under a modern farming system during four months postpartum.
32821668	9	91	theme	milk	1243:1246	arg1	samples					1248:1254	milk samples	1243:1254	milk samples	1243:1254	Collection of milk samples started in the 2nd week of postpartum and continued over biweekly intervals up to 16 weeks to determine major fat, protein, lactose, solid non-fat (SNF), and density percentages.
32123204	8	0	theme	potential	1518:1526	arg1	mechanisms					1538:1547	potential molecular mechanisms	1518:1547	potential molecular mechanisms responsible for the attenuation of colitis by certain compounds of tobacco	1518:1622	However, the inverse association between active smoking and the development of UC cannot be ignored and the present study lays the foundation for investigating potential molecular mechanisms responsible for the attenuation of colitis by certain compounds of tobacco when decoupled from combustion.
32123204	2	1	theme	Bowel	336:340	arg1	IBD					351:353	IBD	351:353	IBD	351:353	The impact of CS on Inflammatory Bowel Disease (IBD) has been established by a number of epidemiological and clinical studies.
32123204	2	1	theme	Bowel	336:340	arg1	Disease					342:348	Inflammatory Bowel Disease	323:348	Inflammatory Bowel Disease (IBD)	323:354	The impact of CS on Inflammatory Bowel Disease (IBD) has been established by a number of epidemiological and clinical studies.
32123204	5	2	theme	gut	1020:1022	arg1	microbiome					1024:1033	the gut microbiome	1016:1033	the gut microbiome	1016:1033	Colon transcriptome analysis revealed that CS downregulated specific pathways in a concentration-dependent manner, affecting both the inflammatory state and composition of the gut microbiome.
32123204	8	3	theme	certain	1595:1601	arg1	compounds					1603:1611	certain compounds	1595:1611	certain compounds of tobacco	1595:1622	However, the inverse association between active smoking and the development of UC cannot be ignored and the present study lays the foundation for investigating potential molecular mechanisms responsible for the attenuation of colitis by certain compounds of tobacco when decoupled from combustion.
32123204	0	4	theme	cigarette	65:73	arg1	smoke					75:79	cigarette smoke	65:79	cigarette smoke	65:79	The reduction of DSS-induced colitis severity in mice exposed to cigarette smoke is linked to immune modulation and microbial shifts.
32123204	0	5	from	reduction	4:12	arg1	mice					49:52	mice	49:52	mice exposed to cigarette smoke	49:79	The reduction of DSS-induced colitis severity in mice exposed to cigarette smoke is linked to immune modulation and microbial shifts.
32123204	1	6	theme	cigarette	146:154	arg1	CS					163:164	CS	163:164	CS	163:164	Exposure to cigarette smoke (CS) causes detrimental health effects, increasing the risk of cardiovascular, pulmonary diseases and carcinogenesis in exposed individuals.
32123204	1	6	theme	cigarette	146:154	arg1	smoke					156:160	cigarette smoke	146:160	cigarette smoke (CS)	146:165	Exposure to cigarette smoke (CS) causes detrimental health effects, increasing the risk of cardiovascular, pulmonary diseases and carcinogenesis in exposed individuals.
32123204	1	7	theme	cardiovascular	225:238	arg1	risk					217:220	the risk	213:220	the risk of cardiovascular, pulmonary diseases and carcinogenesis in exposed individuals	213:300	Exposure to cigarette smoke (CS) causes detrimental health effects, increasing the risk of cardiovascular, pulmonary diseases and carcinogenesis in exposed individuals.
32123204	8	8	theme	present	1466:1472	arg1	study					1474:1478	the present study	1462:1478	the present study	1462:1478	However, the inverse association between active smoking and the development of UC cannot be ignored and the present study lays the foundation for investigating potential molecular mechanisms responsible for the attenuation of colitis by certain compounds of tobacco when decoupled from combustion.
32123204	2	9	from	impact	307:312	arg1	IBD					351:353	IBD	351:353	IBD	351:353	The impact of CS on Inflammatory Bowel Disease (IBD) has been established by a number of epidemiological and clinical studies.
32123204	2	9	from	impact	307:312	arg1	Disease					342:348	Inflammatory Bowel Disease	323:348	Inflammatory Bowel Disease (IBD)	323:354	The impact of CS on Inflammatory Bowel Disease (IBD) has been established by a number of epidemiological and clinical studies.
32123204	6	10	theme	genera	1136:1141	arg1	dysbiosis					1104:1112	DSS-induced dysbiosis	1092:1112	DSS-induced dysbiosis of specific bacterial genera	1092:1141	Metagenomics analysis demonstrated that CS can modulate DSS-induced dysbiosis of specific bacterial genera, contributing to resolve the inflammation or accelerate recovery.
32123204	4	11	theme	exposure	651:658	arg1	effect					638:643	the effect	634:643	the effect of CS exposure on experimental colitis	634:682	To investigate the effect of CS exposure on experimental colitis, we performed a comprehensive and integrated comparative analysis of colon transcriptome and microbiome in mice exposed to dextran sodium sulfate (DSS) and CS.
32123204	8	12	theme	active	1399:1404	arg1	smoking					1406:1412	active smoking	1399:1412	active smoking	1399:1412	However, the inverse association between active smoking and the development of UC cannot be ignored and the present study lays the foundation for investigating potential molecular mechanisms responsible for the attenuation of colitis by certain compounds of tobacco when decoupled from combustion.
32123204	4	13	from	microbiome	777:786	arg1	mice					791:794	mice	791:794	mice exposed to dextran sodium sulfate (DSS) and CS	791:841	To investigate the effect of CS exposure on experimental colitis, we performed a comprehensive and integrated comparative analysis of colon transcriptome and microbiome in mice exposed to dextran sodium sulfate (DSS) and CS.
32123204	6	14	theme	bacterial	1126:1134	arg1	genera					1136:1141	specific bacterial genera	1117:1141	specific bacterial genera	1117:1141	Metagenomics analysis demonstrated that CS can modulate DSS-induced dysbiosis of specific bacterial genera, contributing to resolve the inflammation or accelerate recovery.
32123204	4	15	theme	experimental	663:674	arg1	colitis					676:682	experimental colitis	663:682	experimental colitis	663:682	To investigate the effect of CS exposure on experimental colitis, we performed a comprehensive and integrated comparative analysis of colon transcriptome and microbiome in mice exposed to dextran sodium sulfate (DSS) and CS.
32123204	7	16	theme	significant	1329:1339	arg1	benefits					1348:1355	its significant health benefits	1325:1355	its significant health benefits	1325:1355	The risks of smoking far outweigh any possible benefit, thus smoking cessation must always be encouraged because of its significant health benefits.
32123204	1	17	theme	pulmonary	241:249	arg1	diseases					251:258	pulmonary diseases	241:258	pulmonary diseases	241:258	Exposure to cigarette smoke (CS) causes detrimental health effects, increasing the risk of cardiovascular, pulmonary diseases and carcinogenesis in exposed individuals.
32123204	0	18	theme	immune	94:99	arg1	modulation					101:110	immune modulation	94:110	immune modulation	94:110	The reduction of DSS-induced colitis severity in mice exposed to cigarette smoke is linked to immune modulation and microbial shifts.
32123204	5	19	theme	microbiome	1024:1033	arg1	composition					1001:1011	composition	1001:1011	composition	1001:1011	Colon transcriptome analysis revealed that CS downregulated specific pathways in a concentration-dependent manner, affecting both the inflammatory state and composition of the gut microbiome.
32123204	5	19	theme	microbiome	1024:1033	arg1	state					991:995	the inflammatory state	974:995	the inflammatory state	974:995	Colon transcriptome analysis revealed that CS downregulated specific pathways in a concentration-dependent manner, affecting both the inflammatory state and composition of the gut microbiome.
32123204	4	20	theme	transcriptome	759:771	arg1	analysis					741:748	a comprehensive and integrated comparative analysis	698:748	a comprehensive and integrated comparative analysis of colon transcriptome and microbiome in mice exposed to dextran sodium sulfate (DSS) and CS	698:841	To investigate the effect of CS exposure on experimental colitis, we performed a comprehensive and integrated comparative analysis of colon transcriptome and microbiome in mice exposed to dextran sodium sulfate (DSS) and CS.
32123204	3	21	dep	development	546:556	arg1	the					542:544	the	542:544	the	542:544	In fact, CS is associated with a higher risk of developing Crohn's disease (CD) while inversely correlates with the development, disease risks, and relapse rate of ulcerative colitis (UC).
32123204	6	22	theme	specific	1117:1124	arg1	genera					1136:1141	specific bacterial genera	1117:1141	specific bacterial genera	1117:1141	Metagenomics analysis demonstrated that CS can modulate DSS-induced dysbiosis of specific bacterial genera, contributing to resolve the inflammation or accelerate recovery.
32123204	4	23	from	transcriptome	759:771	arg1	mice					791:794	mice	791:794	mice exposed to dextran sodium sulfate (DSS) and CS	791:841	To investigate the effect of CS exposure on experimental colitis, we performed a comprehensive and integrated comparative analysis of colon transcriptome and microbiome in mice exposed to dextran sodium sulfate (DSS) and CS.
32123204	4	24	theme	colon	753:757	arg1	transcriptome					759:771	colon transcriptome	753:771	colon transcriptome	753:771	To investigate the effect of CS exposure on experimental colitis, we performed a comprehensive and integrated comparative analysis of colon transcriptome and microbiome in mice exposed to dextran sodium sulfate (DSS) and CS.
32123204	3	25	theme	developing	478:487	arg1	CD					506:507	CD	506:507	CD	506:507	In fact, CS is associated with a higher risk of developing Crohn's disease (CD) while inversely correlates with the development, disease risks, and relapse rate of ulcerative colitis (UC).
32123204	3	25	theme	developing	478:487	arg1	disease					497:503	Crohn's disease	489:503	developing Crohn's disease (CD)	478:508	In fact, CS is associated with a higher risk of developing Crohn's disease (CD) while inversely correlates with the development, disease risks, and relapse rate of ulcerative colitis (UC).
32123204	2	26	theme	clinical	412:419	arg1	studies					421:427	epidemiological and clinical studies	392:427	epidemiological and clinical studies	392:427	The impact of CS on Inflammatory Bowel Disease (IBD) has been established by a number of epidemiological and clinical studies.
32123204	4	27	theme	microbiome	777:786	arg1	analysis					741:748	a comprehensive and integrated comparative analysis	698:748	a comprehensive and integrated comparative analysis of colon transcriptome and microbiome in mice exposed to dextran sodium sulfate (DSS) and CS	698:841	To investigate the effect of CS exposure on experimental colitis, we performed a comprehensive and integrated comparative analysis of colon transcriptome and microbiome in mice exposed to dextran sodium sulfate (DSS) and CS.
32123204	4	28	theme	CS	648:649	arg1	exposure					651:658	CS exposure	648:658	CS exposure	648:658	To investigate the effect of CS exposure on experimental colitis, we performed a comprehensive and integrated comparative analysis of colon transcriptome and microbiome in mice exposed to dextran sodium sulfate (DSS) and CS.
32123204	4	29	theme	dextran	807:813	arg1	DSS					831:833	dextran sodium sulfate (DSS)	807:834	dextran sodium sulfate (DSS)	807:834	To investigate the effect of CS exposure on experimental colitis, we performed a comprehensive and integrated comparative analysis of colon transcriptome and microbiome in mice exposed to dextran sodium sulfate (DSS) and CS.
32123204	4	30	from	mice	791:794	arg1	analysis					741:748	a comprehensive and integrated comparative analysis	698:748	a comprehensive and integrated comparative analysis of colon transcriptome and microbiome in mice exposed to dextran sodium sulfate (DSS) and CS	698:841	To investigate the effect of CS exposure on experimental colitis, we performed a comprehensive and integrated comparative analysis of colon transcriptome and microbiome in mice exposed to dextran sodium sulfate (DSS) and CS.
32123204	1	31	theme	diseases	251:258	arg1	risk					217:220	the risk	213:220	the risk of cardiovascular, pulmonary diseases and carcinogenesis in exposed individuals	213:300	Exposure to cigarette smoke (CS) causes detrimental health effects, increasing the risk of cardiovascular, pulmonary diseases and carcinogenesis in exposed individuals.
32123204	5	32	theme	Colon	844:848	arg1	analysis					864:871	Colon transcriptome analysis	844:871	Colon transcriptome analysis	844:871	Colon transcriptome analysis revealed that CS downregulated specific pathways in a concentration-dependent manner, affecting both the inflammatory state and composition of the gut microbiome.
32123204	7	33	theme	smoking	1270:1276	arg1	cessation					1278:1286	smoking cessation	1270:1286	smoking cessation	1270:1286	The risks of smoking far outweigh any possible benefit, thus smoking cessation must always be encouraged because of its significant health benefits.
32123204	2	34	theme	epidemiological	392:406	arg1	studies					421:427	epidemiological and clinical studies	392:427	epidemiological and clinical studies	392:427	The impact of CS on Inflammatory Bowel Disease (IBD) has been established by a number of epidemiological and clinical studies.
32123204	3	35	theme	disease	497:503	arg1	risk					470:473	a higher risk	461:473	a higher risk of developing Crohn's disease (CD)	461:508	In fact, CS is associated with a higher risk of developing Crohn's disease (CD) while inversely correlates with the development, disease risks, and relapse rate of ulcerative colitis (UC).
32123204	5	36	theme	concentration-dependent	927:949	arg1	manner					951:956	a concentration-dependent manner	925:956	a concentration-dependent manner	925:956	Colon transcriptome analysis revealed that CS downregulated specific pathways in a concentration-dependent manner, affecting both the inflammatory state and composition of the gut microbiome.
32123204	6	37	theme	DSS-induced	1092:1102	arg1	dysbiosis					1104:1112	DSS-induced dysbiosis	1092:1112	DSS-induced dysbiosis of specific bacterial genera	1092:1141	Metagenomics analysis demonstrated that CS can modulate DSS-induced dysbiosis of specific bacterial genera, contributing to resolve the inflammation or accelerate recovery.
32123204	1	38	theme	carcinogenesis	264:277	arg1	risk					217:220	the risk	213:220	the risk of cardiovascular, pulmonary diseases and carcinogenesis in exposed individuals	213:300	Exposure to cigarette smoke (CS) causes detrimental health effects, increasing the risk of cardiovascular, pulmonary diseases and carcinogenesis in exposed individuals.
32123204	2	39	theme	Inflammatory	323:334	arg1	IBD					351:353	IBD	351:353	IBD	351:353	The impact of CS on Inflammatory Bowel Disease (IBD) has been established by a number of epidemiological and clinical studies.
32123204	2	39	theme	Inflammatory	323:334	arg1	Disease					342:348	Inflammatory Bowel Disease	323:348	Inflammatory Bowel Disease (IBD)	323:354	The impact of CS on Inflammatory Bowel Disease (IBD) has been established by a number of epidemiological and clinical studies.
32123204	1	40	theme	detrimental	174:184	arg1	effects					193:199	detrimental health effects	174:199	detrimental health effects	174:199	Exposure to cigarette smoke (CS) causes detrimental health effects, increasing the risk of cardiovascular, pulmonary diseases and carcinogenesis in exposed individuals.
32123204	0	41	theme	severity	37:44	arg1	reduction					4:12	The reduction	0:12	The reduction of DSS-induced colitis severity in mice exposed to cigarette smoke	0:79	The reduction of DSS-induced colitis severity in mice exposed to cigarette smoke is linked to immune modulation and microbial shifts.
32123204	3	42	theme	relapse	578:584	arg1	rate					586:589	relapse rate	578:589	relapse rate	578:589	In fact, CS is associated with a higher risk of developing Crohn's disease (CD) while inversely correlates with the development, disease risks, and relapse rate of ulcerative colitis (UC).
32123204	4	43	theme	comparative	729:739	arg1	analysis					741:748	a comprehensive and integrated comparative analysis	698:748	a comprehensive and integrated comparative analysis of colon transcriptome and microbiome in mice exposed to dextran sodium sulfate (DSS) and CS	698:841	To investigate the effect of CS exposure on experimental colitis, we performed a comprehensive and integrated comparative analysis of colon transcriptome and microbiome in mice exposed to dextran sodium sulfate (DSS) and CS.
32123204	1	44	theme	health	186:191	arg1	effects					193:199	detrimental health effects	174:199	detrimental health effects	174:199	Exposure to cigarette smoke (CS) causes detrimental health effects, increasing the risk of cardiovascular, pulmonary diseases and carcinogenesis in exposed individuals.
32123204	0	45	theme	colitis	29:35	arg1	severity					37:44	DSS-induced colitis severity	17:44	DSS-induced colitis severity	17:44	The reduction of DSS-induced colitis severity in mice exposed to cigarette smoke is linked to immune modulation and microbial shifts.
32123204	1	46	theme	exposed	282:288	arg1	individuals					290:300	exposed individuals	282:300	exposed individuals	282:300	Exposure to cigarette smoke (CS) causes detrimental health effects, increasing the risk of cardiovascular, pulmonary diseases and carcinogenesis in exposed individuals.
32123204	0	47	theme	microbial	116:124	arg1	shifts					126:131	microbial shifts	116:131	microbial shifts	116:131	The reduction of DSS-induced colitis severity in mice exposed to cigarette smoke is linked to immune modulation and microbial shifts.
32123204	0	48	theme	DSS-induced	17:27	arg1	severity					37:44	DSS-induced colitis severity	17:44	DSS-induced colitis severity	17:44	The reduction of DSS-induced colitis severity in mice exposed to cigarette smoke is linked to immune modulation and microbial shifts.
32123204	7	49	theme	possible	1247:1254	arg1	benefit					1256:1262	any possible benefit	1243:1262	any possible benefit	1243:1262	The risks of smoking far outweigh any possible benefit, thus smoking cessation must always be encouraged because of its significant health benefits.
32123204	8	50	theme	molecular	1528:1536	arg1	mechanisms					1538:1547	potential molecular mechanisms	1518:1547	potential molecular mechanisms responsible for the attenuation of colitis by certain compounds of tobacco	1518:1622	However, the inverse association between active smoking and the development of UC cannot be ignored and the present study lays the foundation for investigating potential molecular mechanisms responsible for the attenuation of colitis by certain compounds of tobacco when decoupled from combustion.
32123204	4	51	theme	sodium	815:820	arg1	DSS					831:833	dextran sodium sulfate (DSS)	807:834	dextran sodium sulfate (DSS)	807:834	To investigate the effect of CS exposure on experimental colitis, we performed a comprehensive and integrated comparative analysis of colon transcriptome and microbiome in mice exposed to dextran sodium sulfate (DSS) and CS.
32123204	4	52	from	analysis	741:748	arg1	mice					791:794	mice	791:794	mice exposed to dextran sodium sulfate (DSS) and CS	791:841	To investigate the effect of CS exposure on experimental colitis, we performed a comprehensive and integrated comparative analysis of colon transcriptome and microbiome in mice exposed to dextran sodium sulfate (DSS) and CS.
32123204	2	53	theme	studies	421:427	arg1	number					382:387	a number	380:387	a number of epidemiological and clinical studies	380:427	The impact of CS on Inflammatory Bowel Disease (IBD) has been established by a number of epidemiological and clinical studies.
32123204	1	54	from	risk	217:220	arg1	individuals					290:300	exposed individuals	282:300	exposed individuals	282:300	Exposure to cigarette smoke (CS) causes detrimental health effects, increasing the risk of cardiovascular, pulmonary diseases and carcinogenesis in exposed individuals.
32123204	3	55	theme	ulcerative	594:603	arg1	UC					614:615	UC	614:615	UC	614:615	In fact, CS is associated with a higher risk of developing Crohn's disease (CD) while inversely correlates with the development, disease risks, and relapse rate of ulcerative colitis (UC).
32123204	3	55	theme	ulcerative	594:603	arg1	colitis					605:611	ulcerative colitis	594:611	ulcerative colitis (UC)	594:616	In fact, CS is associated with a higher risk of developing Crohn's disease (CD) while inversely correlates with the development, disease risks, and relapse rate of ulcerative colitis (UC).
32123204	2	56	theme	CS	317:318	arg1	impact					307:312	The impact	303:312	The impact of CS on Inflammatory Bowel Disease (IBD)	303:354	The impact of CS on Inflammatory Bowel Disease (IBD) has been established by a number of epidemiological and clinical studies.
32123204	4	57	from	effect	638:643	arg1	colitis					676:682	experimental colitis	663:682	experimental colitis	663:682	To investigate the effect of CS exposure on experimental colitis, we performed a comprehensive and integrated comparative analysis of colon transcriptome and microbiome in mice exposed to dextran sodium sulfate (DSS) and CS.
32123204	8	58	theme	responsible	1549:1559	arg1	mechanisms					1538:1547	potential molecular mechanisms	1518:1547	potential molecular mechanisms responsible for the attenuation of colitis by certain compounds of tobacco	1518:1622	However, the inverse association between active smoking and the development of UC cannot be ignored and the present study lays the foundation for investigating potential molecular mechanisms responsible for the attenuation of colitis by certain compounds of tobacco when decoupled from combustion.
32123204	3	59	theme	colitis	605:611	arg1	development					546:556	development	546:556	development	546:556	In fact, CS is associated with a higher risk of developing Crohn's disease (CD) while inversely correlates with the development, disease risks, and relapse rate of ulcerative colitis (UC).
32123204	3	59	theme	colitis	605:611	arg1	risks					567:571	disease risks	559:571	disease risks	559:571	In fact, CS is associated with a higher risk of developing Crohn's disease (CD) while inversely correlates with the development, disease risks, and relapse rate of ulcerative colitis (UC).
32123204	3	59	theme	colitis	605:611	arg1	rate					586:589	relapse rate	578:589	relapse rate	578:589	In fact, CS is associated with a higher risk of developing Crohn's disease (CD) while inversely correlates with the development, disease risks, and relapse rate of ulcerative colitis (UC).
32123204	5	60	theme	specific	904:911	arg1	pathways					913:920	specific pathways	904:920	specific pathways	904:920	Colon transcriptome analysis revealed that CS downregulated specific pathways in a concentration-dependent manner, affecting both the inflammatory state and composition of the gut microbiome.
32123204	4	61	theme	integrated	718:727	arg1	analysis					741:748	a comprehensive and integrated comparative analysis	698:748	a comprehensive and integrated comparative analysis of colon transcriptome and microbiome in mice exposed to dextran sodium sulfate (DSS) and CS	698:841	To investigate the effect of CS exposure on experimental colitis, we performed a comprehensive and integrated comparative analysis of colon transcriptome and microbiome in mice exposed to dextran sodium sulfate (DSS) and CS.
32123204	8	62	theme	inverse	1371:1377	arg1	association					1379:1389	the inverse association	1367:1389	the inverse association between active smoking and the development of UC	1367:1438	However, the inverse association between active smoking and the development of UC cannot be ignored and the present study lays the foundation for investigating potential molecular mechanisms responsible for the attenuation of colitis by certain compounds of tobacco when decoupled from combustion.
32123204	5	63	theme	transcriptome	850:862	arg1	analysis					864:871	Colon transcriptome analysis	844:871	Colon transcriptome analysis	844:871	Colon transcriptome analysis revealed that CS downregulated specific pathways in a concentration-dependent manner, affecting both the inflammatory state and composition of the gut microbiome.
32123204	4	64	theme	sulfate	822:828	arg1	DSS					831:833	dextran sodium sulfate (DSS)	807:834	dextran sodium sulfate (DSS)	807:834	To investigate the effect of CS exposure on experimental colitis, we performed a comprehensive and integrated comparative analysis of colon transcriptome and microbiome in mice exposed to dextran sodium sulfate (DSS) and CS.
32123204	5	65	theme	inflammatory	978:989	arg1	state					991:995	the inflammatory state	974:995	the inflammatory state	974:995	Colon transcriptome analysis revealed that CS downregulated specific pathways in a concentration-dependent manner, affecting both the inflammatory state and composition of the gut microbiome.
32123204	3	66	theme	disease	559:565	arg1	risks					567:571	disease risks	559:571	disease risks	559:571	In fact, CS is associated with a higher risk of developing Crohn's disease (CD) while inversely correlates with the development, disease risks, and relapse rate of ulcerative colitis (UC).
32123204	7	67	theme	smoking	1222:1228	arg1	risks					1213:1217	The risks	1209:1217	The risks of smoking	1209:1228	The risks of smoking far outweigh any possible benefit, thus smoking cessation must always be encouraged because of its significant health benefits.
32123204	8	68	theme	UC	1437:1438	arg1	smoking					1406:1412	active smoking	1399:1412	active smoking	1399:1412	However, the inverse association between active smoking and the development of UC cannot be ignored and the present study lays the foundation for investigating potential molecular mechanisms responsible for the attenuation of colitis by certain compounds of tobacco when decoupled from combustion.
32123204	8	68	theme	UC	1437:1438	arg1	development					1422:1432	the development	1418:1432	the development of UC	1418:1438	However, the inverse association between active smoking and the development of UC cannot be ignored and the present study lays the foundation for investigating potential molecular mechanisms responsible for the attenuation of colitis by certain compounds of tobacco when decoupled from combustion.
32123204	0	69	attach	linked	84:89	arg1	modulation					101:110	immune modulation	94:110	immune modulation	94:110	The reduction of DSS-induced colitis severity in mice exposed to cigarette smoke is linked to immune modulation and microbial shifts.
32123204	0	69	attach	linked	84:89	arg1	shifts					126:131	microbial shifts	116:131	microbial shifts	116:131	The reduction of DSS-induced colitis severity in mice exposed to cigarette smoke is linked to immune modulation and microbial shifts.
32123204	0	69	attach	linked	84:89	arg2	reduction					4:12	The reduction	0:12	The reduction of DSS-induced colitis severity in mice exposed to cigarette smoke	0:79	The reduction of DSS-induced colitis severity in mice exposed to cigarette smoke is linked to immune modulation and microbial shifts.
32123204	3	70	theme	higher	463:468	arg1	risk					470:473	a higher risk	461:473	a higher risk of developing Crohn's disease (CD)	461:508	In fact, CS is associated with a higher risk of developing Crohn's disease (CD) while inversely correlates with the development, disease risks, and relapse rate of ulcerative colitis (UC).
32123204	8	71	theme	tobacco	1616:1622	arg1	compounds					1603:1611	certain compounds	1595:1611	certain compounds of tobacco	1595:1622	However, the inverse association between active smoking and the development of UC cannot be ignored and the present study lays the foundation for investigating potential molecular mechanisms responsible for the attenuation of colitis by certain compounds of tobacco when decoupled from combustion.
32123204	8	72	theme	colitis	1584:1590	arg1	attenuation					1569:1579	the attenuation	1565:1579	the attenuation of colitis by certain compounds of tobacco	1565:1622	However, the inverse association between active smoking and the development of UC cannot be ignored and the present study lays the foundation for investigating potential molecular mechanisms responsible for the attenuation of colitis by certain compounds of tobacco when decoupled from combustion.
32123204	6	73	theme	Metagenomics	1036:1047	arg1	analysis					1049:1056	Metagenomics analysis	1036:1056	Metagenomics analysis	1036:1056	Metagenomics analysis demonstrated that CS can modulate DSS-induced dysbiosis of specific bacterial genera, contributing to resolve the inflammation or accelerate recovery.
32123204	4	74	theme	comprehensive	700:712	arg1	analysis					741:748	a comprehensive and integrated comparative analysis	698:748	a comprehensive and integrated comparative analysis of colon transcriptome and microbiome in mice exposed to dextran sodium sulfate (DSS) and CS	698:841	To investigate the effect of CS exposure on experimental colitis, we performed a comprehensive and integrated comparative analysis of colon transcriptome and microbiome in mice exposed to dextran sodium sulfate (DSS) and CS.
32123204	7	75	theme	health	1341:1346	arg1	benefits					1348:1355	its significant health benefits	1325:1355	its significant health benefits	1325:1355	The risks of smoking far outweigh any possible benefit, thus smoking cessation must always be encouraged because of its significant health benefits.
32977599	0	0	theme	Ebola	79:83	arg1	Particles					96:104	Ebola Virus-Like Particles	79:104	Ebola Virus-Like Particles	79:104	Expression of a Large Single-Chain 13F6 Antibody with Binding Activity against Ebola Virus-Like Particles in a Plant System.
32977599	3	1	theme	different	291:299	arg1	antibodies					523:532	inexpensive and effective diagnostic antibodies	486:532	inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD)	486:566	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	3	1	theme	different	291:299	arg1	antibodies					317:326	two structurally different anti-Ebola 13F6 antibodies	274:326	two structurally different anti-Ebola 13F6 antibodies	274:326	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	1	2	theme	human	147:151	arg1	viruses					153:159	Pathogenic animal and human viruses	125:159	Pathogenic animal and human viruses	125:159	Pathogenic animal and human viruses present a growing and persistent threat to humans worldwide.
32977599	6	3	from	antibodies	1058:1067	arg1	tabacum					1075:1081	N. tabacum	1072:1081	N. tabacum	1072:1081	Agrobacterium-mediated plant transformation was employed to express the antibodies in N. tabacum.
32977599	3	4	theme	anti-Ebola	301:310	arg1	antibodies					523:532	inexpensive and effective diagnostic antibodies	486:532	inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD)	486:566	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	3	4	theme	anti-Ebola	301:310	arg1	antibodies					317:326	two structurally different anti-Ebola 13F6 antibodies	274:326	two structurally different anti-Ebola 13F6 antibodies	274:326	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	4	5	theme	mAb	756:758	arg1	antibody					746:753	single-chain (LSC) antibody	727:753	a large single-chain (LSC) antibody (mAb 13F6-LSC)	719:768	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	4	5	theme	mAb	756:758	arg1	13F6-LSC					760:767	mAb 13F6-LSC	756:767	mAb 13F6-LSC	756:767	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	8	6	theme	N-glycan	1294:1301	arg1	structures					1303:1312	high-mannose type N-glycan structures	1276:1312	high-mannose type N-glycan structures	1276:1312	The antibodies tagged with the KDEL motif displayed high-mannose type N-glycan structures and efficient binding to EBOV-like particles (VLPs).
32977599	9	7	theme	anti-EBOV	1390:1398	arg1	13F6-FULL					1419:1427	13F6-FULL	1419:1427	13F6-FULL	1419:1427	Thus, various forms of anti-EBOV plant-derived mAbs 13F6-FULL and LSC with efficient binding affinity to EBOV VLP can be produced in the plant system.
32977599	9	7	theme	anti-EBOV	1390:1398	arg1	mAbs					1414:1417	anti-EBOV plant-derived mAbs 13F6-FULL and LSC	1390:1435	anti-EBOV plant-derived mAbs 13F6-FULL and LSC with efficient binding affinity to EBOV VLP	1390:1479	Thus, various forms of anti-EBOV plant-derived mAbs 13F6-FULL and LSC with efficient binding affinity to EBOV VLP can be produced in the plant system.
32977599	9	7	theme	anti-EBOV	1390:1398	arg1	LSC					1433:1435	LSC	1433:1435	LSC	1433:1435	Thus, various forms of anti-EBOV plant-derived mAbs 13F6-FULL and LSC with efficient binding affinity to EBOV VLP can be produced in the plant system.
32977599	5	8	theme	LC	820:821	arg1	region					832:837	the 13F6 LC variable region	811:837	the 13F6 LC variable region (VL)	811:842	mAb 13F6-LSC was constructed by linking the 13F6 LC variable region (VL) with the HC of mAb 13F6-FULL using a peptide linker and extended to the C-terminus using the endoplasmic reticulum (ER) retention motif KDEL.
32977599	5	8	theme	LC	820:821	arg1	VL					840:841	VL	840:841	VL	840:841	mAb 13F6-LSC was constructed by linking the 13F6 LC variable region (VL) with the HC of mAb 13F6-FULL using a peptide linker and extended to the C-terminus using the endoplasmic reticulum (ER) retention motif KDEL.
32977599	6	9	theme	N.	1072:1073	arg1	tabacum					1075:1081	N. tabacum	1072:1081	N. tabacum	1072:1081	Agrobacterium-mediated plant transformation was employed to express the antibodies in N. tabacum.
32977599	9	10	theme	EBOV	1472:1475	arg1	VLP					1477:1479	EBOV VLP	1472:1479	EBOV VLP	1472:1479	Thus, various forms of anti-EBOV plant-derived mAbs 13F6-FULL and LSC with efficient binding affinity to EBOV VLP can be produced in the plant system.
32977599	3	11	theme	diagnostic	512:521	arg1	antibodies					523:532	inexpensive and effective diagnostic antibodies	486:532	inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD)	486:566	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	3	11	theme	diagnostic	512:521	arg1	antibodies					317:326	two structurally different anti-Ebola 13F6 antibodies	274:326	two structurally different anti-Ebola 13F6 antibodies	274:326	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	6	12	theme	Agrobacterium-mediated	986:1007	arg1	transformation					1015:1028	Agrobacterium-mediated plant transformation	986:1028	Agrobacterium-mediated plant transformation	986:1028	Agrobacterium-mediated plant transformation was employed to express the antibodies in N. tabacum.
32977599	0	13	theme	Virus-Like	85:94	arg1	Particles					96:104	Ebola Virus-Like Particles	79:104	Ebola Virus-Like Particles	79:104	Expression of a Large Single-Chain 13F6 Antibody with Binding Activity against Ebola Virus-Like Particles in a Plant System.
32977599	5	14	theme	reticulum	949:957	arg1	motif					974:978	the endoplasmic reticulum (ER) retention motif	933:978	the endoplasmic reticulum (ER) retention motif KDEL	933:983	mAb 13F6-LSC was constructed by linking the 13F6 LC variable region (VL) with the HC of mAb 13F6-FULL using a peptide linker and extended to the C-terminus using the endoplasmic reticulum (ER) retention motif KDEL.
32977599	9	15	theme	mAbs	1414:1417	arg1	forms					1381:1385	various forms	1373:1385	various forms of anti-EBOV plant-derived mAbs 13F6-FULL and LSC with efficient binding affinity to EBOV VLP	1373:1479	Thus, various forms of anti-EBOV plant-derived mAbs 13F6-FULL and LSC with efficient binding affinity to EBOV VLP can be produced in the plant system.
32977599	4	16	theme	light	643:647	arg1	antibody					672:679	monoclonal antibody	661:679	monoclonal antibody	661:679	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	4	16	theme	light	643:647	arg1	LC					656:657	LC	656:657	LC	656:657	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	4	16	theme	light	643:647	arg1	chain					649:653	light chain	643:653	light chain (LC) (monoclonal antibody, mAb 13F6-FULL)	643:695	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	3	17	theme	Nicotiana	445:453	arg1	plants					463:468	transgenic Nicotiana tabacum plants	434:468	transgenic Nicotiana tabacum plants	434:468	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	9	18	link	plant-derived	1400:1412	arg1	13F6-FULL					1419:1427	13F6-FULL	1419:1427	13F6-FULL	1419:1427	Thus, various forms of anti-EBOV plant-derived mAbs 13F6-FULL and LSC with efficient binding affinity to EBOV VLP can be produced in the plant system.
32977599	9	18	link	plant-derived	1400:1412	arg1	mAbs					1414:1417	anti-EBOV plant-derived mAbs 13F6-FULL and LSC	1390:1435	anti-EBOV plant-derived mAbs 13F6-FULL and LSC with efficient binding affinity to EBOV VLP	1390:1479	Thus, various forms of anti-EBOV plant-derived mAbs 13F6-FULL and LSC with efficient binding affinity to EBOV VLP can be produced in the plant system.
32977599	9	18	link	plant-derived	1400:1412	arg1	LSC					1433:1435	LSC	1433:1435	LSC	1433:1435	Thus, various forms of anti-EBOV plant-derived mAbs 13F6-FULL and LSC with efficient binding affinity to EBOV VLP can be produced in the plant system.
32977599	7	19	theme	gene	1135:1138	arg1	transcription					1151:1163	the gene insertion, transcription, and protein expression	1131:1187	transcription	1151:1163	PCR, RT-PCR, and immunoblot analyses confirmed the gene insertion, transcription, and protein expression of these antibodies, respectively.
32977599	4	20	theme	monoclonal	661:670	arg1	antibody					672:679	monoclonal antibody	661:679	monoclonal antibody	661:679	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	4	20	theme	monoclonal	661:670	arg1	chain					649:653	light chain	643:653	light chain (LC) (monoclonal antibody, mAb 13F6-FULL)	643:695	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	9	21	theme	binding	1452:1458	arg1	affinity					1460:1467	efficient binding affinity	1442:1467	efficient binding affinity to EBOV VLP	1442:1479	Thus, various forms of anti-EBOV plant-derived mAbs 13F6-FULL and LSC with efficient binding affinity to EBOV VLP can be produced in the plant system.
32977599	3	22	theme	glycoprotein	397:408	arg1	domain					377:382	the heavily glycosylated mucin-like domain	341:382	the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP)	341:413	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	3	22	theme	glycoprotein	397:408	arg1	glycoprotein					397:408	the glycoprotein	393:408	the glycoprotein (GP)	393:413	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	3	22	theme	glycoprotein	397:408	arg1	MLD					385:387	MLD	385:387	MLD	385:387	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	4	23	theme	heavy	622:626	arg1	HC					635:636	HC	635:636	HC	635:636	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	4	23	theme	heavy	622:626	arg1	chain					628:632	heavy chain	622:632	heavy chain (HC)	622:637	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	9	24	with	mAbs	1414:1417	arg1	affinity					1460:1467	efficient binding affinity	1442:1467	efficient binding affinity to EBOV VLP	1442:1479	Thus, various forms of anti-EBOV plant-derived mAbs 13F6-FULL and LSC with efficient binding affinity to EBOV VLP can be produced in the plant system.
32977599	9	25	theme	various	1373:1379	arg1	forms					1381:1385	various forms	1373:1385	various forms of anti-EBOV plant-derived mAbs 13F6-FULL and LSC with efficient binding affinity to EBOV VLP	1373:1479	Thus, various forms of anti-EBOV plant-derived mAbs 13F6-FULL and LSC with efficient binding affinity to EBOV VLP can be produced in the plant system.
32977599	3	26	theme	effective	502:510	arg1	antibodies					523:532	inexpensive and effective diagnostic antibodies	486:532	inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD)	486:566	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	3	26	theme	effective	502:510	arg1	antibodies					317:326	two structurally different anti-Ebola 13F6 antibodies	274:326	two structurally different anti-Ebola 13F6 antibodies	274:326	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	9	27	dep	mAbs	1414:1417	arg1	13F6-FULL					1419:1427	13F6-FULL	1419:1427	13F6-FULL	1419:1427	Thus, various forms of anti-EBOV plant-derived mAbs 13F6-FULL and LSC with efficient binding affinity to EBOV VLP can be produced in the plant system.
32977599	9	27	dep	mAbs	1414:1417	arg1	mAbs					1414:1417	anti-EBOV plant-derived mAbs 13F6-FULL and LSC	1390:1435	anti-EBOV plant-derived mAbs 13F6-FULL and LSC with efficient binding affinity to EBOV VLP	1390:1479	Thus, various forms of anti-EBOV plant-derived mAbs 13F6-FULL and LSC with efficient binding affinity to EBOV VLP can be produced in the plant system.
32977599	9	27	dep	mAbs	1414:1417	arg1	LSC					1433:1435	LSC	1433:1435	LSC	1433:1435	Thus, various forms of anti-EBOV plant-derived mAbs 13F6-FULL and LSC with efficient binding affinity to EBOV VLP can be produced in the plant system.
32977599	3	28	theme	glycosylated	353:364	arg1	domain					377:382	the heavily glycosylated mucin-like domain	341:382	the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP)	341:413	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	3	28	theme	glycosylated	353:364	arg1	glycoprotein					397:408	the glycoprotein	393:408	the glycoprotein (GP)	393:413	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	3	28	theme	glycosylated	353:364	arg1	MLD					385:387	MLD	385:387	MLD	385:387	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	3	29	theme	virus	548:552	arg1	EVD					563:565	EVD	563:565	EVD	563:565	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	3	29	theme	virus	548:552	arg1	disease					554:560	Ebola virus disease	542:560	Ebola virus disease (EVD)	542:566	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	1	30	theme	growing	171:177	arg1	threat					194:199	a growing and persistent threat	169:199	a growing and persistent threat to humans worldwide	169:219	Pathogenic animal and human viruses present a growing and persistent threat to humans worldwide.
32977599	3	31	gly	glycosylated	353:364	arg1	domain					377:382	the heavily glycosylated mucin-like domain	341:382	the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP)	341:413	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	3	31	gly	glycosylated	353:364	arg1	glycoprotein					397:408	the glycoprotein	393:408	the glycoprotein (GP)	393:413	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	3	31	gly	glycosylated	353:364	arg1	MLD					385:387	MLD	385:387	MLD	385:387	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	5	32	theme	variable	823:830	arg1	region					832:837	the 13F6 LC variable region	811:837	the 13F6 LC variable region (VL)	811:842	mAb 13F6-LSC was constructed by linking the 13F6 LC variable region (VL) with the HC of mAb 13F6-FULL using a peptide linker and extended to the C-terminus using the endoplasmic reticulum (ER) retention motif KDEL.
32977599	5	32	theme	variable	823:830	arg1	VL					840:841	VL	840:841	VL	840:841	mAb 13F6-LSC was constructed by linking the 13F6 LC variable region (VL) with the HC of mAb 13F6-FULL using a peptide linker and extended to the C-terminus using the endoplasmic reticulum (ER) retention motif KDEL.
32977599	8	33	theme	high-mannose	1276:1287	arg1	structures					1303:1312	high-mannose type N-glycan structures	1276:1312	high-mannose type N-glycan structures	1276:1312	The antibodies tagged with the KDEL motif displayed high-mannose type N-glycan structures and efficient binding to EBOV-like particles (VLPs).
32977599	9	34	theme	plant	1504:1508	arg1	system					1510:1515	the plant system	1500:1515	the plant system	1500:1515	Thus, various forms of anti-EBOV plant-derived mAbs 13F6-FULL and LSC with efficient binding affinity to EBOV VLP can be produced in the plant system.
32977599	0	35	theme	Large	16:20	arg1	Antibody					40:47	a Large Single-Chain 13F6 Antibody	14:47	a Large Single-Chain 13F6 Antibody	14:47	Expression of a Large Single-Chain 13F6 Antibody with Binding Activity against Ebola Virus-Like Particles in a Plant System.
32977599	7	36	theme	antibodies	1198:1207	arg1	expression					1178:1187	the gene insertion, transcription, and protein expression	1131:1187	expression	1178:1187	PCR, RT-PCR, and immunoblot analyses confirmed the gene insertion, transcription, and protein expression of these antibodies, respectively.
32977599	7	36	theme	antibodies	1198:1207	arg1	transcription					1151:1163	the gene insertion, transcription, and protein expression	1131:1187	transcription	1151:1163	PCR, RT-PCR, and immunoblot analyses confirmed the gene insertion, transcription, and protein expression of these antibodies, respectively.
32977599	5	37	theme	13F6	815:818	arg1	region					832:837	the 13F6 LC variable region	811:837	the 13F6 LC variable region (VL)	811:842	mAb 13F6-LSC was constructed by linking the 13F6 LC variable region (VL) with the HC of mAb 13F6-FULL using a peptide linker and extended to the C-terminus using the endoplasmic reticulum (ER) retention motif KDEL.
32977599	5	37	theme	13F6	815:818	arg1	VL					840:841	VL	840:841	VL	840:841	mAb 13F6-LSC was constructed by linking the 13F6 LC variable region (VL) with the HC of mAb 13F6-FULL using a peptide linker and extended to the C-terminus using the endoplasmic reticulum (ER) retention motif KDEL.
32977599	6	38	theme	plant	1009:1013	arg1	transformation					1015:1028	Agrobacterium-mediated plant transformation	986:1028	Agrobacterium-mediated plant transformation	986:1028	Agrobacterium-mediated plant transformation was employed to express the antibodies in N. tabacum.
32977599	8	39	theme	KDEL	1255:1258	arg1	motif					1260:1264	the KDEL motif	1251:1264	the KDEL motif	1251:1264	The antibodies tagged with the KDEL motif displayed high-mannose type N-glycan structures and efficient binding to EBOV-like particles (VLPs).
32977599	1	40	theme	persistent	183:192	arg1	threat					194:199	a growing and persistent threat	169:199	a growing and persistent threat to humans worldwide	169:219	Pathogenic animal and human viruses present a growing and persistent threat to humans worldwide.
32977599	3	41	theme	mucin-like	366:375	arg1	domain					377:382	the heavily glycosylated mucin-like domain	341:382	the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP)	341:413	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	3	41	theme	mucin-like	366:375	arg1	glycoprotein					397:408	the glycoprotein	393:408	the glycoprotein (GP)	393:413	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	3	41	theme	mucin-like	366:375	arg1	MLD					385:387	MLD	385:387	MLD	385:387	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	0	42	theme	Plant	111:115	arg1	System					117:122	a Plant System	109:122	a Plant System	109:122	Expression of a Large Single-Chain 13F6 Antibody with Binding Activity against Ebola Virus-Like Particles in a Plant System.
32977599	8	43	theme	efficient	1318:1326	arg1	binding					1328:1334	efficient binding	1318:1334	efficient binding to EBOV-like particles (VLPs)	1318:1364	The antibodies tagged with the KDEL motif displayed high-mannose type N-glycan structures and efficient binding to EBOV-like particles (VLPs).
32977599	3	44	theme	Ebola	542:546	arg1	EVD					563:565	EVD	563:565	EVD	563:565	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	3	44	theme	Ebola	542:546	arg1	disease					554:560	Ebola virus disease	542:560	Ebola virus disease (EVD)	542:566	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	4	45	theme	13F6	593:596	arg1	first					573:577	first	573:577	first	573:577	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	4	45	theme	13F6	593:596	arg1	antibody					608:615	anti-EBOV 13F6 full size antibody	583:615	anti-EBOV 13F6 full size antibody	583:615	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	0	46	theme	13F6	35:38	arg1	Antibody					40:47	a Large Single-Chain 13F6 Antibody	14:47	a Large Single-Chain 13F6 Antibody	14:47	Expression of a Large Single-Chain 13F6 Antibody with Binding Activity against Ebola Virus-Like Particles in a Plant System.
32977599	5	47	dep	motif	974:978	arg1	KDEL					980:983	KDEL	980:983	the endoplasmic reticulum (ER) retention motif KDEL	933:983	mAb 13F6-LSC was constructed by linking the 13F6 LC variable region (VL) with the HC of mAb 13F6-FULL using a peptide linker and extended to the C-terminus using the endoplasmic reticulum (ER) retention motif KDEL.
32977599	9	48	theme	efficient	1442:1450	arg1	affinity					1460:1467	efficient binding affinity	1442:1467	efficient binding affinity to EBOV VLP	1442:1479	Thus, various forms of anti-EBOV plant-derived mAbs 13F6-FULL and LSC with efficient binding affinity to EBOV VLP can be produced in the plant system.
32977599	7	49	theme	immunoblot	1101:1110	arg1	analyses					1112:1119	immunoblot analyses	1101:1119	immunoblot analyses	1101:1119	PCR, RT-PCR, and immunoblot analyses confirmed the gene insertion, transcription, and protein expression of these antibodies, respectively.
32977599	4	50	theme	large	721:725	arg1	antibody					746:753	single-chain (LSC) antibody	727:753	a large single-chain (LSC) antibody (mAb 13F6-LSC)	719:768	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	4	50	theme	large	721:725	arg1	13F6-LSC					760:767	mAb 13F6-LSC	756:767	mAb 13F6-LSC	756:767	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	4	50	theme	large	721:725	arg1	LSC					741:743	LSC	741:743	LSC	741:743	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	4	50	theme	large	721:725	arg1	second					708:713	second	708:713	second	708:713	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	0	51	theme	Single-Chain	22:33	arg1	Antibody					40:47	a Large Single-Chain 13F6 Antibody	14:47	a Large Single-Chain 13F6 Antibody	14:47	Expression of a Large Single-Chain 13F6 Antibody with Binding Activity against Ebola Virus-Like Particles in a Plant System.
32977599	0	52	from	Activity	62:69	arg1	System					117:122	a Plant System	109:122	a Plant System	109:122	Expression of a Large Single-Chain 13F6 Antibody with Binding Activity against Ebola Virus-Like Particles in a Plant System.
32977599	2	53	theme	Ebola	222:226	arg1	EBOV					235:238	EBOV	235:238	EBOV	235:238	Ebola virus (EBOV) causes zoonosis in humans.
32977599	2	53	theme	Ebola	222:226	arg1	virus					228:232	Ebola virus	222:232	Ebola virus (EBOV)	222:239	Ebola virus (EBOV) causes zoonosis in humans.
32977599	1	54	theme	humans	204:209	arg1	worldwide					211:219	humans worldwide	204:219	humans worldwide	204:219	Pathogenic animal and human viruses present a growing and persistent threat to humans worldwide.
32977599	5	55	theme	mAb	771:773	arg1	13F6-LSC					775:782	mAb 13F6-LSC	771:782	mAb 13F6-LSC	771:782	mAb 13F6-LSC was constructed by linking the 13F6 LC variable region (VL) with the HC of mAb 13F6-FULL using a peptide linker and extended to the C-terminus using the endoplasmic reticulum (ER) retention motif KDEL.
32977599	5	56	theme	endoplasmic	937:947	arg1	ER					960:961	ER	960:961	ER	960:961	mAb 13F6-LSC was constructed by linking the 13F6 LC variable region (VL) with the HC of mAb 13F6-FULL using a peptide linker and extended to the C-terminus using the endoplasmic reticulum (ER) retention motif KDEL.
32977599	5	56	theme	endoplasmic	937:947	arg1	reticulum					949:957	the endoplasmic reticulum	933:957	the endoplasmic reticulum (ER) retention motif KDEL	933:983	mAb 13F6-LSC was constructed by linking the 13F6 LC variable region (VL) with the HC of mAb 13F6-FULL using a peptide linker and extended to the C-terminus using the endoplasmic reticulum (ER) retention motif KDEL.
32977599	5	57	theme	peptide	881:887	arg1	linker					889:894	a peptide linker	879:894	a peptide linker	879:894	mAb 13F6-LSC was constructed by linking the 13F6 LC variable region (VL) with the HC of mAb 13F6-FULL using a peptide linker and extended to the C-terminus using the endoplasmic reticulum (ER) retention motif KDEL.
32977599	3	58	theme	tabacum	455:461	arg1	plants					463:468	transgenic Nicotiana tabacum plants	434:468	transgenic Nicotiana tabacum plants	434:468	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	0	59	theme	Antibody	40:47	arg1	Expression					0:9	Expression	0:9	Expression of a Large Single-Chain 13F6 Antibody with Binding Activity against Ebola Virus-Like Particles in a Plant System.	0:123	Expression of a Large Single-Chain 13F6 Antibody with Binding Activity against Ebola Virus-Like Particles in a Plant System.
32977599	4	60	with	antibody	608:615	arg1	antibody					672:679	monoclonal antibody	661:679	monoclonal antibody	661:679	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	4	60	with	antibody	608:615	arg1	LC					656:657	LC	656:657	LC	656:657	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	4	60	with	antibody	608:615	arg1	HC					635:636	HC	635:636	HC	635:636	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	4	60	with	antibody	608:615	arg1	chain					628:632	heavy chain	622:632	heavy chain (HC)	622:637	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	4	60	with	antibody	608:615	arg1	chain					649:653	light chain	643:653	light chain (LC) (monoclonal antibody, mAb 13F6-FULL)	643:695	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	4	61	theme	single-chain	727:738	arg1	antibody					746:753	single-chain (LSC) antibody	727:753	a large single-chain (LSC) antibody (mAb 13F6-LSC)	719:768	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	4	61	theme	single-chain	727:738	arg1	13F6-LSC					760:767	mAb 13F6-LSC	756:767	mAb 13F6-LSC	756:767	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	4	61	theme	single-chain	727:738	arg1	LSC					741:743	LSC	741:743	LSC	741:743	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	4	61	theme	single-chain	727:738	arg1	second					708:713	second	708:713	second	708:713	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	4	62	dep	antibody	672:679	arg1	mAb					682:684	mAb 13F6-FULL	682:694	mAb 13F6-FULL	682:694	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	5	63	theme	13F6-FULL	863:871	arg1	HC					853:854	the HC	849:854	the HC of mAb 13F6-FULL using a peptide linker	849:894	mAb 13F6-LSC was constructed by linking the 13F6 LC variable region (VL) with the HC of mAb 13F6-FULL using a peptide linker and extended to the C-terminus using the endoplasmic reticulum (ER) retention motif KDEL.
32977599	8	64	theme	EBOV-like	1339:1347	arg1	particles					1349:1357	EBOV-like particles	1339:1357	EBOV-like particles (VLPs)	1339:1364	The antibodies tagged with the KDEL motif displayed high-mannose type N-glycan structures and efficient binding to EBOV-like particles (VLPs).
32977599	8	64	theme	EBOV-like	1339:1347	arg1	VLPs					1360:1363	VLPs	1360:1363	VLPs	1360:1363	The antibodies tagged with the KDEL motif displayed high-mannose type N-glycan structures and efficient binding to EBOV-like particles (VLPs).
32977599	8	65	theme	type	1289:1292	arg1	structures					1303:1312	high-mannose type N-glycan structures	1276:1312	high-mannose type N-glycan structures	1276:1312	The antibodies tagged with the KDEL motif displayed high-mannose type N-glycan structures and efficient binding to EBOV-like particles (VLPs).
32977599	0	66	with	Expression	0:9	arg1	Activity					62:69	Binding Activity	54:69	Binding Activity against Ebola Virus-Like Particles in a Plant System	54:122	Expression of a Large Single-Chain 13F6 Antibody with Binding Activity against Ebola Virus-Like Particles in a Plant System.
32977599	3	67	theme	transgenic	434:443	arg1	plants					463:468	transgenic Nicotiana tabacum plants	434:468	transgenic Nicotiana tabacum plants	434:468	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	2	68	from	zoonosis	248:255	arg1	humans					260:265	humans	260:265	humans	260:265	Ebola virus (EBOV) causes zoonosis in humans.
32977599	7	69	theme	insertion	1140:1148	arg1	transcription					1151:1163	the gene insertion, transcription, and protein expression	1131:1187	transcription	1151:1163	PCR, RT-PCR, and immunoblot analyses confirmed the gene insertion, transcription, and protein expression of these antibodies, respectively.
32977599	4	70	theme	13F6-FULL	686:694	arg1	mAb					682:684	mAb 13F6-FULL	682:694	mAb 13F6-FULL	682:694	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	1	71	theme	Pathogenic	125:134	arg1	viruses					153:159	Pathogenic animal and human viruses	125:159	Pathogenic animal and human viruses	125:159	Pathogenic animal and human viruses present a growing and persistent threat to humans worldwide.
32977599	7	72	theme	protein	1170:1176	arg1	expression					1178:1187	the gene insertion, transcription, and protein expression	1131:1187	expression	1178:1187	PCR, RT-PCR, and immunoblot analyses confirmed the gene insertion, transcription, and protein expression of these antibodies, respectively.
32977599	3	73	theme	13F6	312:315	arg1	antibodies					523:532	inexpensive and effective diagnostic antibodies	486:532	inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD)	486:566	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	3	73	theme	13F6	312:315	arg1	antibodies					317:326	two structurally different anti-Ebola 13F6 antibodies	274:326	two structurally different anti-Ebola 13F6 antibodies	274:326	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	4	74	theme	size	603:606	arg1	first					573:577	first	573:577	first	573:577	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	4	74	theme	size	603:606	arg1	antibody					608:615	anti-EBOV 13F6 full size antibody	583:615	anti-EBOV 13F6 full size antibody	583:615	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	5	75	theme	mAb	859:861	arg1	13F6-FULL					863:871	mAb 13F6-FULL	859:871	mAb 13F6-FULL using a peptide linker	859:894	mAb 13F6-LSC was constructed by linking the 13F6 LC variable region (VL) with the HC of mAb 13F6-FULL using a peptide linker and extended to the C-terminus using the endoplasmic reticulum (ER) retention motif KDEL.
32977599	4	76	theme	full	598:601	arg1	first					573:577	first	573:577	first	573:577	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	4	76	theme	full	598:601	arg1	antibody					608:615	anti-EBOV 13F6 full size antibody	583:615	anti-EBOV 13F6 full size antibody	583:615	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	1	77	theme	animal	136:141	arg1	viruses					153:159	Pathogenic animal and human viruses	125:159	Pathogenic animal and human viruses	125:159	Pathogenic animal and human viruses present a growing and persistent threat to humans worldwide.
32977599	4	78	theme	anti-EBOV	583:591	arg1	first					573:577	first	573:577	first	573:577	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	4	78	theme	anti-EBOV	583:591	arg1	antibody					608:615	anti-EBOV 13F6 full size antibody	583:615	anti-EBOV 13F6 full size antibody	583:615	The first was anti-EBOV 13F6 full size antibody with heavy chain (HC) and light chain (LC) (monoclonal antibody, mAb 13F6-FULL), while the second was a large single-chain (LSC) antibody (mAb 13F6-LSC).
32977599	0	79	theme	Binding	54:60	arg1	Activity					62:69	Binding Activity	54:69	Binding Activity against Ebola Virus-Like Particles in a Plant System	54:122	Expression of a Large Single-Chain 13F6 Antibody with Binding Activity against Ebola Virus-Like Particles in a Plant System.
32977599	5	80	theme	retention	964:972	arg1	motif					974:978	the endoplasmic reticulum (ER) retention motif	933:978	the endoplasmic reticulum (ER) retention motif KDEL	933:983	mAb 13F6-LSC was constructed by linking the 13F6 LC variable region (VL) with the HC of mAb 13F6-FULL using a peptide linker and extended to the C-terminus using the endoplasmic reticulum (ER) retention motif KDEL.
32977599	3	81	gly	glycoprotein	397:408	arg1	GP					411:412	GP	411:412	GP	411:412	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	3	81	gly	glycoprotein	397:408	arg1	glycoprotein					397:408	the glycoprotein	393:408	the glycoprotein (GP)	393:413	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	9	82	theme	plant-derived	1400:1412	arg1	13F6-FULL					1419:1427	13F6-FULL	1419:1427	13F6-FULL	1419:1427	Thus, various forms of anti-EBOV plant-derived mAbs 13F6-FULL and LSC with efficient binding affinity to EBOV VLP can be produced in the plant system.
32977599	9	82	theme	plant-derived	1400:1412	arg1	mAbs					1414:1417	anti-EBOV plant-derived mAbs 13F6-FULL and LSC	1390:1435	anti-EBOV plant-derived mAbs 13F6-FULL and LSC with efficient binding affinity to EBOV VLP	1390:1479	Thus, various forms of anti-EBOV plant-derived mAbs 13F6-FULL and LSC with efficient binding affinity to EBOV VLP can be produced in the plant system.
32977599	9	82	theme	plant-derived	1400:1412	arg1	LSC					1433:1435	LSC	1433:1435	LSC	1433:1435	Thus, various forms of anti-EBOV plant-derived mAbs 13F6-FULL and LSC with efficient binding affinity to EBOV VLP can be produced in the plant system.
32977599	3	83	theme	inexpensive	486:496	arg1	antibodies					523:532	inexpensive and effective diagnostic antibodies	486:532	inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD)	486:566	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
32977599	3	83	theme	inexpensive	486:496	arg1	antibodies					317:326	two structurally different anti-Ebola 13F6 antibodies	274:326	two structurally different anti-Ebola 13F6 antibodies	274:326	Here, two structurally different anti-Ebola 13F6 antibodies, recognizing the heavily glycosylated mucin-like domain (MLD) of the glycoprotein (GP), were expressed in transgenic Nicotiana tabacum plants and designed as inexpensive and effective diagnostic antibodies against Ebola virus disease (EVD).
33726981	6	0	theme	various	1259:1265	arg1	analytes					1267:1274	various analytes	1259:1274	various analytes	1259:1274	To validate the robustness of the method, we performed the analysis by 1:1 mixing of normal and isotope-labeled glycans, and obtained excellent linear calibration curves from various analytes.
33726981	2	1	theme	stable	357:362	arg1	isotope					364:370	stable isotope	357:370	stable isotope	357:370	In order to decode the correlation of glycan prevalence with their physiological contribution, many mass spectrometry (MS) and stable isotope labeling-based methods have been developed for the relative quantification of glycans.
33726981	7	2	theme	glycan	1378:1383	arg1	profile					1396:1402	glycan expression profile	1378:1402	glycan expression profile in yeast	1378:1411	Finally, we quantitated the inhibitory effect of tunicamycin, a N-linked glycosylation inhibitor, to glycan expression profile in yeast.
33726981	1	3	gly	glycoproteins	166:178	arg1	glycoproteins					166:178	glycoproteins	166:178	glycoproteins	166:178	Changes in glycan levels could directly affect the biochemical properties of glycoproteins and thus influence their physiological functions.
33726981	5	4	theme	isotope-labeled	910:924	arg1	glucose					926:932	isotope-labeled glucose	910:932	isotope-labeled glucose	910:932	We established that metabolic incorporation of isotope-labeled glucose at a concentration of 5 mg/mL for three days is required for an accurate quantitative analysis with optimal isotopic cluster distribution of glycans.
33726981	7	5	theme	N-linked	1341:1348	arg1	inhibitor					1364:1372	a N-linked glycosylation inhibitor	1339:1372	a N-linked glycosylation inhibitor	1339:1372	Finally, we quantitated the inhibitory effect of tunicamycin, a N-linked glycosylation inhibitor, to glycan expression profile in yeast.
33726981	7	5	theme	N-linked	1341:1348	arg1	tunicamycin					1326:1336	tunicamycin	1326:1336	tunicamycin	1326:1336	Finally, we quantitated the inhibitory effect of tunicamycin, a N-linked glycosylation inhibitor, to glycan expression profile in yeast.
33726981	5	6	theme	accurate	998:1005	arg1	analysis					1020:1027	an accurate quantitative analysis	995:1027	an accurate quantitative analysis with optimal isotopic cluster distribution of glycans	995:1081	We established that metabolic incorporation of isotope-labeled glucose at a concentration of 5 mg/mL for three days is required for an accurate quantitative analysis with optimal isotopic cluster distribution of glycans.
33726981	7	7	theme	expression	1385:1394	arg1	profile					1396:1402	glycan expression profile	1378:1402	glycan expression profile in yeast	1378:1411	Finally, we quantitated the inhibitory effect of tunicamycin, a N-linked glycosylation inhibitor, to glycan expression profile in yeast.
33726981	5	8	theme	isotopic	1042:1049	arg1	distribution					1059:1070	optimal isotopic cluster distribution	1034:1070	optimal isotopic cluster distribution of glycans	1034:1081	We established that metabolic incorporation of isotope-labeled glucose at a concentration of 5 mg/mL for three days is required for an accurate quantitative analysis with optimal isotopic cluster distribution of glycans.
33726981	2	9	theme	physiological	297:309	arg1	contribution					311:322	their physiological contribution	291:322	their physiological contribution	291:322	In order to decode the correlation of glycan prevalence with their physiological contribution, many mass spectrometry (MS) and stable isotope labeling-based methods have been developed for the relative quantification of glycans.
33726981	4	10	theme	carbon-13	743:751	arg1	1-13C1					770:775	1-13C1	770:775	1-13C1	770:775	We demonstrate that culturing baker's yeast in the presence of carbon-13 labeled glucose (1-13C1) leads to effective incorporation of carbon-13 to both N-linked and O-linked glycans.
33726981	4	10	theme	carbon-13	743:751	arg1	glucose					761:767	carbon-13 labeled glucose	743:767	carbon-13 labeled glucose (1-13C1)	743:776	We demonstrate that culturing baker's yeast in the presence of carbon-13 labeled glucose (1-13C1) leads to effective incorporation of carbon-13 to both N-linked and O-linked glycans.
33726981	3	11	theme	Polysaccharides	579:593	arg1	Labeling					567:574	optimized Metabolic Isotope Labeling	539:574	optimized Metabolic Isotope Labeling of Polysaccharides with Isotopic Glucose (MILPIG) approach in baker's yeast (Saccharomyces cerevisiae)	539:677	In this study, we expand the quantitative glycomic toolbox with the addition of optimized Metabolic Isotope Labeling of Polysaccharides with Isotopic Glucose (MILPIG) approach in baker's yeast (Saccharomyces cerevisiae).
33726981	4	12	theme	glucose	761:767	arg1	presence					731:738	the presence	727:738	the presence of carbon-13 labeled glucose (1-13C1)	727:776	We demonstrate that culturing baker's yeast in the presence of carbon-13 labeled glucose (1-13C1) leads to effective incorporation of carbon-13 to both N-linked and O-linked glycans.
33726981	3	13	theme	Isotope	559:565	arg1	Labeling					567:574	optimized Metabolic Isotope Labeling	539:574	optimized Metabolic Isotope Labeling of Polysaccharides with Isotopic Glucose (MILPIG) approach in baker's yeast (Saccharomyces cerevisiae)	539:677	In this study, we expand the quantitative glycomic toolbox with the addition of optimized Metabolic Isotope Labeling of Polysaccharides with Isotopic Glucose (MILPIG) approach in baker's yeast (Saccharomyces cerevisiae).
33726981	5	14	theme	glucose	926:932	arg1	incorporation					893:905	metabolic incorporation	883:905	metabolic incorporation of isotope-labeled glucose at a concentration of 5 mg/mL for three days	883:977	We established that metabolic incorporation of isotope-labeled glucose at a concentration of 5 mg/mL for three days is required for an accurate quantitative analysis with optimal isotopic cluster distribution of glycans.
33726981	2	15	theme	relative	423:430	arg1	quantification					432:445	the relative quantification	419:445	the relative quantification of glycans	419:456	In order to decode the correlation of glycan prevalence with their physiological contribution, many mass spectrometry (MS) and stable isotope labeling-based methods have been developed for the relative quantification of glycans.
33726981	1	16	theme	glycoproteins	166:178	arg1	properties					152:161	the biochemical properties	136:161	the biochemical properties of glycoproteins	136:178	Changes in glycan levels could directly affect the biochemical properties of glycoproteins and thus influence their physiological functions.
33726981	7	17	theme	tunicamycin	1326:1336	arg1	effect					1316:1321	the inhibitory effect	1301:1321	the inhibitory effect of tunicamycin, a N-linked glycosylation inhibitor, to glycan expression profile in yeast	1301:1411	Finally, we quantitated the inhibitory effect of tunicamycin, a N-linked glycosylation inhibitor, to glycan expression profile in yeast.
33726981	4	18	theme	N-linked	832:839	arg1	glycans					854:860	both N-linked and O-linked glycans	827:860	both N-linked and O-linked glycans	827:860	We demonstrate that culturing baker's yeast in the presence of carbon-13 labeled glucose (1-13C1) leads to effective incorporation of carbon-13 to both N-linked and O-linked glycans.
33726981	6	19	theme	1:1	1155:1157	arg1	mixing					1159:1164	1:1 mixing	1155:1164	1:1 mixing of normal and isotope-labeled glycans	1155:1202	To validate the robustness of the method, we performed the analysis by 1:1 mixing of normal and isotope-labeled glycans, and obtained excellent linear calibration curves from various analytes.
33726981	2	20	theme	prevalence	275:284	arg1	correlation					253:263	the correlation	249:263	the correlation of glycan prevalence with their physiological contribution	249:322	In order to decode the correlation of glycan prevalence with their physiological contribution, many mass spectrometry (MS) and stable isotope labeling-based methods have been developed for the relative quantification of glycans.
33726981	7	21	theme	glycosylation	1350:1362	arg1	inhibitor					1364:1372	a N-linked glycosylation inhibitor	1339:1372	a N-linked glycosylation inhibitor	1339:1372	Finally, we quantitated the inhibitory effect of tunicamycin, a N-linked glycosylation inhibitor, to glycan expression profile in yeast.
33726981	7	21	theme	glycosylation	1350:1362	arg1	tunicamycin					1326:1336	tunicamycin	1326:1336	tunicamycin	1326:1336	Finally, we quantitated the inhibitory effect of tunicamycin, a N-linked glycosylation inhibitor, to glycan expression profile in yeast.
33726981	7	22	link	N-linked	1341:1348	arg1	inhibitor					1364:1372	a N-linked glycosylation inhibitor	1339:1372	a N-linked glycosylation inhibitor	1339:1372	Finally, we quantitated the inhibitory effect of tunicamycin, a N-linked glycosylation inhibitor, to glycan expression profile in yeast.
33726981	7	22	link	N-linked	1341:1348	arg1	tunicamycin					1326:1336	tunicamycin	1326:1336	tunicamycin	1326:1336	Finally, we quantitated the inhibitory effect of tunicamycin, a N-linked glycosylation inhibitor, to glycan expression profile in yeast.
33726981	5	23	from	concentration	939:951	arg1	incorporation					893:905	metabolic incorporation	883:905	metabolic incorporation of isotope-labeled glucose at a concentration of 5 mg/mL for three days	883:977	We established that metabolic incorporation of isotope-labeled glucose at a concentration of 5 mg/mL for three days is required for an accurate quantitative analysis with optimal isotopic cluster distribution of glycans.
33726981	2	24	theme	mass	330:333	arg1	MS					349:350	MS	349:350	MS	349:350	In order to decode the correlation of glycan prevalence with their physiological contribution, many mass spectrometry (MS) and stable isotope labeling-based methods have been developed for the relative quantification of glycans.
33726981	2	24	theme	mass	330:333	arg1	spectrometry					335:346	mass spectrometry	330:346	mass spectrometry (MS)	330:351	In order to decode the correlation of glycan prevalence with their physiological contribution, many mass spectrometry (MS) and stable isotope labeling-based methods have been developed for the relative quantification of glycans.
33726981	0	25	theme	Metabolic	0:8	arg1	labeling					10:17	Metabolic labeling	0:17	Metabolic labeling of glycans with isotopic glucose for quantitative glycomics in yeast.	0:87	Metabolic labeling of glycans with isotopic glucose for quantitative glycomics in yeast.
33726981	4	26	theme	O-linked	845:852	arg1	glycans					854:860	both N-linked and O-linked glycans	827:860	both N-linked and O-linked glycans	827:860	We demonstrate that culturing baker's yeast in the presence of carbon-13 labeled glucose (1-13C1) leads to effective incorporation of carbon-13 to both N-linked and O-linked glycans.
33726981	2	27	theme	many	325:328	arg1	methods					387:393	many mass spectrometry (MS) and stable isotope labeling-based methods	325:393	many mass spectrometry (MS) and stable isotope labeling-based methods	325:393	In order to decode the correlation of glycan prevalence with their physiological contribution, many mass spectrometry (MS) and stable isotope labeling-based methods have been developed for the relative quantification of glycans.
33726981	0	28	theme	glycans	22:28	arg1	labeling					10:17	Metabolic labeling	0:17	Metabolic labeling of glycans with isotopic glucose for quantitative glycomics in yeast.	0:87	Metabolic labeling of glycans with isotopic glucose for quantitative glycomics in yeast.
33726981	3	29	theme	Glucose	609:615	arg1	approach					626:633	Isotopic Glucose (MILPIG) approach	600:633	Isotopic Glucose (MILPIG) approach in baker's yeast (Saccharomyces cerevisiae)	600:677	In this study, we expand the quantitative glycomic toolbox with the addition of optimized Metabolic Isotope Labeling of Polysaccharides with Isotopic Glucose (MILPIG) approach in baker's yeast (Saccharomyces cerevisiae).
33726981	3	30	theme	Labeling	567:574	arg1	addition					527:534	the addition	523:534	the addition of optimized Metabolic Isotope Labeling of Polysaccharides with Isotopic Glucose (MILPIG) approach in baker's yeast (Saccharomyces cerevisiae)	523:677	In this study, we expand the quantitative glycomic toolbox with the addition of optimized Metabolic Isotope Labeling of Polysaccharides with Isotopic Glucose (MILPIG) approach in baker's yeast (Saccharomyces cerevisiae).
33726981	7	31	from	profile	1396:1402	arg1	yeast					1407:1411	yeast	1407:1411	yeast	1407:1411	Finally, we quantitated the inhibitory effect of tunicamycin, a N-linked glycosylation inhibitor, to glycan expression profile in yeast.
33726981	5	32	theme	5 mg/mL	956:962	arg1	concentration					939:951	a concentration	937:951	a concentration of 5 mg/mL for three days	937:977	We established that metabolic incorporation of isotope-labeled glucose at a concentration of 5 mg/mL for three days is required for an accurate quantitative analysis with optimal isotopic cluster distribution of glycans.
33726981	6	33	theme	glycans	1196:1202	arg1	mixing					1159:1164	1:1 mixing	1155:1164	1:1 mixing of normal and isotope-labeled glycans	1155:1202	To validate the robustness of the method, we performed the analysis by 1:1 mixing of normal and isotope-labeled glycans, and obtained excellent linear calibration curves from various analytes.
33726981	1	34	theme	glycan	100:105	arg1	levels					107:112	glycan levels	100:112	glycan levels	100:112	Changes in glycan levels could directly affect the biochemical properties of glycoproteins and thus influence their physiological functions.
33726981	1	35	theme	physiological	205:217	arg1	functions					219:227	their physiological functions	199:227	their physiological functions	199:227	Changes in glycan levels could directly affect the biochemical properties of glycoproteins and thus influence their physiological functions.
33726981	0	36	theme	isotopic	35:42	arg1	glucose					44:50	isotopic glucose	35:50	isotopic glucose for quantitative glycomics in yeast	35:86	Metabolic labeling of glycans with isotopic glucose for quantitative glycomics in yeast.
33726981	6	37	theme	calibration	1235:1245	arg1	curves					1247:1252	excellent linear calibration curves	1218:1252	excellent linear calibration curves	1218:1252	To validate the robustness of the method, we performed the analysis by 1:1 mixing of normal and isotope-labeled glycans, and obtained excellent linear calibration curves from various analytes.
33726981	4	38	theme	carbon-13	814:822	arg1	incorporation					797:809	effective incorporation	787:809	effective incorporation of carbon-13 to both N-linked and O-linked glycans	787:860	We demonstrate that culturing baker's yeast in the presence of carbon-13 labeled glucose (1-13C1) leads to effective incorporation of carbon-13 to both N-linked and O-linked glycans.
33726981	7	39	theme	inhibitory	1305:1314	arg1	effect					1316:1321	the inhibitory effect	1301:1321	the inhibitory effect of tunicamycin, a N-linked glycosylation inhibitor, to glycan expression profile in yeast	1301:1411	Finally, we quantitated the inhibitory effect of tunicamycin, a N-linked glycosylation inhibitor, to glycan expression profile in yeast.
33726981	4	40	link	O-linked	845:852	arg1	glycans					854:860	both N-linked and O-linked glycans	827:860	both N-linked and O-linked glycans	827:860	We demonstrate that culturing baker's yeast in the presence of carbon-13 labeled glucose (1-13C1) leads to effective incorporation of carbon-13 to both N-linked and O-linked glycans.
33726981	2	41	with	correlation	253:263	arg1	contribution					311:322	their physiological contribution	291:322	their physiological contribution	291:322	In order to decode the correlation of glycan prevalence with their physiological contribution, many mass spectrometry (MS) and stable isotope labeling-based methods have been developed for the relative quantification of glycans.
33726981	0	42	from	glycomics	69:77	arg1	yeast					82:86	yeast	82:86	yeast	82:86	Metabolic labeling of glycans with isotopic glucose for quantitative glycomics in yeast.
33726981	2	43	theme	glycans	450:456	arg1	quantification					432:445	the relative quantification	419:445	the relative quantification of glycans	419:456	In order to decode the correlation of glycan prevalence with their physiological contribution, many mass spectrometry (MS) and stable isotope labeling-based methods have been developed for the relative quantification of glycans.
33726981	5	44	theme	metabolic	883:891	arg1	incorporation					893:905	metabolic incorporation	883:905	metabolic incorporation of isotope-labeled glucose at a concentration of 5 mg/mL for three days	883:977	We established that metabolic incorporation of isotope-labeled glucose at a concentration of 5 mg/mL for three days is required for an accurate quantitative analysis with optimal isotopic cluster distribution of glycans.
33726981	5	45	with	analysis	1020:1027	arg1	distribution					1059:1070	optimal isotopic cluster distribution	1034:1070	optimal isotopic cluster distribution of glycans	1034:1081	We established that metabolic incorporation of isotope-labeled glucose at a concentration of 5 mg/mL for three days is required for an accurate quantitative analysis with optimal isotopic cluster distribution of glycans.
33726981	3	46	theme	Isotopic	600:607	arg1	approach					626:633	Isotopic Glucose (MILPIG) approach	600:633	Isotopic Glucose (MILPIG) approach in baker's yeast (Saccharomyces cerevisiae)	600:677	In this study, we expand the quantitative glycomic toolbox with the addition of optimized Metabolic Isotope Labeling of Polysaccharides with Isotopic Glucose (MILPIG) approach in baker's yeast (Saccharomyces cerevisiae).
33726981	3	47	theme	quantitative	488:499	arg1	toolbox					510:516	the quantitative glycomic toolbox	484:516	the quantitative glycomic toolbox	484:516	In this study, we expand the quantitative glycomic toolbox with the addition of optimized Metabolic Isotope Labeling of Polysaccharides with Isotopic Glucose (MILPIG) approach in baker's yeast (Saccharomyces cerevisiae).
33726981	4	48	link	N-linked	832:839	arg1	glycans					854:860	both N-linked and O-linked glycans	827:860	both N-linked and O-linked glycans	827:860	We demonstrate that culturing baker's yeast in the presence of carbon-13 labeled glucose (1-13C1) leads to effective incorporation of carbon-13 to both N-linked and O-linked glycans.
33726981	6	49	theme	isotope-labeled	1180:1194	arg1	glycans					1196:1202	normal and isotope-labeled glycans	1169:1202	normal and isotope-labeled glycans	1169:1202	To validate the robustness of the method, we performed the analysis by 1:1 mixing of normal and isotope-labeled glycans, and obtained excellent linear calibration curves from various analytes.
33726981	5	50	theme	cluster	1051:1057	arg1	distribution					1059:1070	optimal isotopic cluster distribution	1034:1070	optimal isotopic cluster distribution of glycans	1034:1081	We established that metabolic incorporation of isotope-labeled glucose at a concentration of 5 mg/mL for three days is required for an accurate quantitative analysis with optimal isotopic cluster distribution of glycans.
33726981	1	51	theme	biochemical	140:150	arg1	properties					152:161	the biochemical properties	136:161	the biochemical properties of glycoproteins	136:178	Changes in glycan levels could directly affect the biochemical properties of glycoproteins and thus influence their physiological functions.
33726981	0	52	with	labeling	10:17	arg1	glucose					44:50	isotopic glucose	35:50	isotopic glucose for quantitative glycomics in yeast	35:86	Metabolic labeling of glycans with isotopic glucose for quantitative glycomics in yeast.
33726981	3	53	theme	glycomic	501:508	arg1	toolbox					510:516	the quantitative glycomic toolbox	484:516	the quantitative glycomic toolbox	484:516	In this study, we expand the quantitative glycomic toolbox with the addition of optimized Metabolic Isotope Labeling of Polysaccharides with Isotopic Glucose (MILPIG) approach in baker's yeast (Saccharomyces cerevisiae).
33726981	6	54	theme	linear	1228:1233	arg1	curves					1247:1252	excellent linear calibration curves	1218:1252	excellent linear calibration curves	1218:1252	To validate the robustness of the method, we performed the analysis by 1:1 mixing of normal and isotope-labeled glycans, and obtained excellent linear calibration curves from various analytes.
33726981	3	55	with	Polysaccharides	579:593	arg1	approach					626:633	Isotopic Glucose (MILPIG) approach	600:633	Isotopic Glucose (MILPIG) approach in baker's yeast (Saccharomyces cerevisiae)	600:677	In this study, we expand the quantitative glycomic toolbox with the addition of optimized Metabolic Isotope Labeling of Polysaccharides with Isotopic Glucose (MILPIG) approach in baker's yeast (Saccharomyces cerevisiae).
33726981	6	56	theme	excellent	1218:1226	arg1	curves					1247:1252	excellent linear calibration curves	1218:1252	excellent linear calibration curves	1218:1252	To validate the robustness of the method, we performed the analysis by 1:1 mixing of normal and isotope-labeled glycans, and obtained excellent linear calibration curves from various analytes.
33726981	2	57	theme	glycan	268:273	arg1	prevalence					275:284	glycan prevalence	268:284	glycan prevalence	268:284	In order to decode the correlation of glycan prevalence with their physiological contribution, many mass spectrometry (MS) and stable isotope labeling-based methods have been developed for the relative quantification of glycans.
33726981	5	58	theme	optimal	1034:1040	arg1	distribution					1059:1070	optimal isotopic cluster distribution	1034:1070	optimal isotopic cluster distribution of glycans	1034:1081	We established that metabolic incorporation of isotope-labeled glucose at a concentration of 5 mg/mL for three days is required for an accurate quantitative analysis with optimal isotopic cluster distribution of glycans.
33726981	4	59	theme	labeled	753:759	arg1	1-13C1					770:775	1-13C1	770:775	1-13C1	770:775	We demonstrate that culturing baker's yeast in the presence of carbon-13 labeled glucose (1-13C1) leads to effective incorporation of carbon-13 to both N-linked and O-linked glycans.
33726981	4	59	theme	labeled	753:759	arg1	glucose					761:767	carbon-13 labeled glucose	743:767	carbon-13 labeled glucose (1-13C1)	743:776	We demonstrate that culturing baker's yeast in the presence of carbon-13 labeled glucose (1-13C1) leads to effective incorporation of carbon-13 to both N-linked and O-linked glycans.
33726981	3	60	theme	optimized	539:547	arg1	Labeling					567:574	optimized Metabolic Isotope Labeling	539:574	optimized Metabolic Isotope Labeling of Polysaccharides with Isotopic Glucose (MILPIG) approach in baker's yeast (Saccharomyces cerevisiae)	539:677	In this study, we expand the quantitative glycomic toolbox with the addition of optimized Metabolic Isotope Labeling of Polysaccharides with Isotopic Glucose (MILPIG) approach in baker's yeast (Saccharomyces cerevisiae).
33726981	5	61	theme	glycans	1075:1081	arg1	distribution					1059:1070	optimal isotopic cluster distribution	1034:1070	optimal isotopic cluster distribution of glycans	1034:1081	We established that metabolic incorporation of isotope-labeled glucose at a concentration of 5 mg/mL for three days is required for an accurate quantitative analysis with optimal isotopic cluster distribution of glycans.
33726981	3	62	theme	Metabolic	549:557	arg1	Labeling					567:574	optimized Metabolic Isotope Labeling	539:574	optimized Metabolic Isotope Labeling of Polysaccharides with Isotopic Glucose (MILPIG) approach in baker's yeast (Saccharomyces cerevisiae)	539:677	In this study, we expand the quantitative glycomic toolbox with the addition of optimized Metabolic Isotope Labeling of Polysaccharides with Isotopic Glucose (MILPIG) approach in baker's yeast (Saccharomyces cerevisiae).
33726981	2	63	theme	spectrometry	335:346	arg1	methods					387:393	many mass spectrometry (MS) and stable isotope labeling-based methods	325:393	many mass spectrometry (MS) and stable isotope labeling-based methods	325:393	In order to decode the correlation of glycan prevalence with their physiological contribution, many mass spectrometry (MS) and stable isotope labeling-based methods have been developed for the relative quantification of glycans.
33726981	3	64	dep	yeast	646:650	arg1	cerevisiae					667:676	Saccharomyces cerevisiae	653:676	baker's yeast (Saccharomyces cerevisiae)	638:677	In this study, we expand the quantitative glycomic toolbox with the addition of optimized Metabolic Isotope Labeling of Polysaccharides with Isotopic Glucose (MILPIG) approach in baker's yeast (Saccharomyces cerevisiae).
33726981	6	65	theme	method	1118:1123	arg1	robustness					1100:1109	the robustness	1096:1109	the robustness of the method	1096:1123	To validate the robustness of the method, we performed the analysis by 1:1 mixing of normal and isotope-labeled glycans, and obtained excellent linear calibration curves from various analytes.
33726981	5	66	theme	quantitative	1007:1018	arg1	analysis					1020:1027	an accurate quantitative analysis	995:1027	an accurate quantitative analysis with optimal isotopic cluster distribution of glycans	995:1081	We established that metabolic incorporation of isotope-labeled glucose at a concentration of 5 mg/mL for three days is required for an accurate quantitative analysis with optimal isotopic cluster distribution of glycans.
33726981	6	67	theme	normal	1169:1174	arg1	glycans					1196:1202	normal and isotope-labeled glycans	1169:1202	normal and isotope-labeled glycans	1169:1202	To validate the robustness of the method, we performed the analysis by 1:1 mixing of normal and isotope-labeled glycans, and obtained excellent linear calibration curves from various analytes.
33726981	2	68	theme	labeling-based	372:385	arg1	methods					387:393	many mass spectrometry (MS) and stable isotope labeling-based methods	325:393	many mass spectrometry (MS) and stable isotope labeling-based methods	325:393	In order to decode the correlation of glycan prevalence with their physiological contribution, many mass spectrometry (MS) and stable isotope labeling-based methods have been developed for the relative quantification of glycans.
33726981	1	69	from	Changes	89:95	arg1	levels					107:112	glycan levels	100:112	glycan levels	100:112	Changes in glycan levels could directly affect the biochemical properties of glycoproteins and thus influence their physiological functions.
33726981	0	70	theme	quantitative	56:67	arg1	glycomics					69:77	quantitative glycomics	56:77	quantitative glycomics in yeast	56:86	Metabolic labeling of glycans with isotopic glucose for quantitative glycomics in yeast.
33726981	4	71	theme	effective	787:795	arg1	incorporation					797:809	effective incorporation	787:809	effective incorporation of carbon-13 to both N-linked and O-linked glycans	787:860	We demonstrate that culturing baker's yeast in the presence of carbon-13 labeled glucose (1-13C1) leads to effective incorporation of carbon-13 to both N-linked and O-linked glycans.
33726981	2	72	theme	isotope	364:370	arg1	methods					387:393	many mass spectrometry (MS) and stable isotope labeling-based methods	325:393	many mass spectrometry (MS) and stable isotope labeling-based methods	325:393	In order to decode the correlation of glycan prevalence with their physiological contribution, many mass spectrometry (MS) and stable isotope labeling-based methods have been developed for the relative quantification of glycans.
32459612	2	0	from	components	366:375	arg1	cells					425:429	RP19, B lymphoblastoid cells	402:429	cells	425:429	In the present study, we investigated the role of sialic acid in TAdV-3 entry and characterized the structural components of TAdV-3 receptor(s) on RP19, B lymphoblastoid cells.
32459612	7	1	link	N-linked	1517:1524	arg1	glycoproteins					1535:1547	N-linked membrane glycoproteins	1517:1547	N-linked membrane glycoproteins	1517:1547	We can conclude that terminal sialic acids attached to N-linked membrane glycoproteins on B cells are used for virus attachment and are essential for successful virus infection.
32459612	3	2	theme	acids	467:471	arg1	Removal					432:438	Removal	432:438	Removal of the cell-surface sialic acids by neuraminidases or blocking of sialic acids by wheat germ agglutinin lectin	432:549	Removal of the cell-surface sialic acids by neuraminidases or blocking of sialic acids by wheat germ agglutinin lectin reduced virus infection.
32459612	4	3	theme	amurensis	613:621	arg1	lectin					623:628	Maackia amurensis lectin	605:628	Maackia amurensis lectin	605:628	Pre-incubation of cells with Maackia amurensis lectin or Sambucus nigra agglutinin resulted in virus reduction, suggesting that TAdV-3 uses both α2,3-linked and α2,6-linked sialic acids as attachment receptor.
32459612	3	4	theme	sialic	506:511	arg1	acids					513:517	sialic acids	506:517	sialic acids	506:517	Removal of the cell-surface sialic acids by neuraminidases or blocking of sialic acids by wheat germ agglutinin lectin reduced virus infection.
32459612	7	5	theme	virus	1623:1627	arg1	infection					1629:1637	successful virus infection	1612:1637	successful virus infection	1612:1637	We can conclude that terminal sialic acids attached to N-linked membrane glycoproteins on B cells are used for virus attachment and are essential for successful virus infection.
32459612	5	6	theme	infectivity	792:802	arg1	data					804:807	Virus infectivity data	786:807	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide)	786:1024	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	5	7	gly	sialylated	1099:1108	arg1	receptor					1110:1117	the sialylated receptor	1095:1117	the sialylated receptor	1095:1117	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	3	8	theme	germ	528:531	arg1	agglutinin					533:542	wheat germ agglutinin	522:542	wheat germ agglutinin lectin	522:549	Removal of the cell-surface sialic acids by neuraminidases or blocking of sialic acids by wheat germ agglutinin lectin reduced virus infection.
32459612	1	9	theme	disease	178:184	arg1	agent					150:154	the causative agent	136:154	the causative agent of an immune-mediated disease in turkeys, haemorrhagic enteritis	136:219	Turkey adenovirus 3 (TAdV-3) is the causative agent of an immune-mediated disease in turkeys, haemorrhagic enteritis, through targeting B lymphocytes.
32459612	1	9	theme	disease	178:184	arg1	adenovirus					111:120	Turkey adenovirus 3	104:122	Turkey adenovirus 3 (TAdV-3)	104:131	Turkey adenovirus 3 (TAdV-3) is the causative agent of an immune-mediated disease in turkeys, haemorrhagic enteritis, through targeting B lymphocytes.
32459612	1	10	dep	turkeys	189:195	arg1	haemorrhagic					198:209	haemorrhagic	198:209	haemorrhagic	198:209	Turkey adenovirus 3 (TAdV-3) is the causative agent of an immune-mediated disease in turkeys, haemorrhagic enteritis, through targeting B lymphocytes.
32459612	5	11	gly	glycoprotein	1159:1170	arg1	glycoprotein					1159:1170	a membrane glycoprotein	1148:1170	a membrane glycoprotein	1148:1170	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	4	12	theme	virus	671:675	arg1	reduction					677:685	virus reduction	671:685	virus reduction	671:685	Pre-incubation of cells with Maackia amurensis lectin or Sambucus nigra agglutinin resulted in virus reduction, suggesting that TAdV-3 uses both α2,3-linked and α2,6-linked sialic acids as attachment receptor.
32459612	3	13	theme	virus	559:563	arg1	infection					565:573	virus infection	559:573	virus infection	559:573	Removal of the cell-surface sialic acids by neuraminidases or blocking of sialic acids by wheat germ agglutinin lectin reduced virus infection.
32459612	6	14	theme	biosynthesis	1365:1376	arg1	inhibition					1340:1349	the inhibition	1336:1349	the inhibition of glycolipid biosynthesis	1336:1376	Furthermore, our data, in conjunction with previous findings, implies that the secondary receptor for TAdV-3 is a protein molecule since the inhibition of glycolipid biosynthesis did not affect the virus infection, which was rather reduced by protease treatment.
32459612	2	15	theme	lymphoblastoid	410:423	arg1	cells					425:429	RP19, B lymphoblastoid cells	402:429	cells	425:429	In the present study, we investigated the role of sialic acid in TAdV-3 entry and characterized the structural components of TAdV-3 receptor(s) on RP19, B lymphoblastoid cells.
32459612	4	16	theme	nigra	642:646	arg1	agglutinin					648:657	Sambucus nigra agglutinin	633:657	Sambucus nigra agglutinin	633:657	Pre-incubation of cells with Maackia amurensis lectin or Sambucus nigra agglutinin resulted in virus reduction, suggesting that TAdV-3 uses both α2,3-linked and α2,6-linked sialic acids as attachment receptor.
32459612	7	17	theme	B	1552:1552	arg1	cells					1554:1558	B cells	1552:1558	B cells	1552:1558	We can conclude that terminal sialic acids attached to N-linked membrane glycoproteins on B cells are used for virus attachment and are essential for successful virus infection.
32459612	7	18	used	used	1564:1567	arg2	acids					1499:1503	terminal sialic acids	1483:1503	terminal sialic acids attached to N-linked membrane glycoproteins on B cells	1483:1558	We can conclude that terminal sialic acids attached to N-linked membrane glycoproteins on B cells are used for virus attachment and are essential for successful virus infection.
32459612	2	19	from	role	297:300	arg1	entry					327:331	TAdV-3 entry	320:331	TAdV-3 entry	320:331	In the present study, we investigated the role of sialic acid in TAdV-3 entry and characterized the structural components of TAdV-3 receptor(s) on RP19, B lymphoblastoid cells.
32459612	4	20	theme	α2,3-linked	721:731	arg1	acids					756:760	both α2,3-linked and α2,6-linked sialic acids	716:760	both α2,3-linked and α2,6-linked sialic acids	716:760	Pre-incubation of cells with Maackia amurensis lectin or Sambucus nigra agglutinin resulted in virus reduction, suggesting that TAdV-3 uses both α2,3-linked and α2,6-linked sialic acids as attachment receptor.
32459612	5	21	theme	sialylated	1099:1108	arg1	receptor					1110:1117	the sialylated receptor	1095:1117	the sialylated receptor	1095:1117	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	2	22	theme	s	396:396	arg1	components					366:375	the structural components	351:375	the structural components of TAdV-3 receptor(s) on RP19, B lymphoblastoid cells	351:429	In the present study, we investigated the role of sialic acid in TAdV-3 entry and characterized the structural components of TAdV-3 receptor(s) on RP19, B lymphoblastoid cells.
32459612	0	23	theme	Turkey	0:5	arg1	siadenovirus					23:34	a siadenovirus	21:34	a siadenovirus	21:34	Turkey adenovirus 3, a siadenovirus, uses sialic acid on N-linked glycoproteins as a cellular receptor.
32459612	0	23	theme	Turkey	0:5	arg1	adenovirus					7:16	Turkey adenovirus 3	0:18	Turkey adenovirus 3	0:18	Turkey adenovirus 3, a siadenovirus, uses sialic acid on N-linked glycoproteins as a cellular receptor.
32459612	1	24	theme	B	240:240	arg1	lymphocytes					242:252	B lymphocytes	240:252	B lymphocytes	240:252	Turkey adenovirus 3 (TAdV-3) is the causative agent of an immune-mediated disease in turkeys, haemorrhagic enteritis, through targeting B lymphocytes.
32459612	2	25	theme	receptor	387:394	arg1	s					396:396	TAdV-3 receptor(s)	380:397	TAdV-3 receptor(s)	380:397	In the present study, we investigated the role of sialic acid in TAdV-3 entry and characterized the structural components of TAdV-3 receptor(s) on RP19, B lymphoblastoid cells.
32459612	7	26	attach	attached	1505:1512	arg3	cells					1554:1558	B cells	1552:1558	B cells	1552:1558	We can conclude that terminal sialic acids attached to N-linked membrane glycoproteins on B cells are used for virus attachment and are essential for successful virus infection.
32459612	7	26	attach	attached	1505:1512	arg2	acids					1499:1503	terminal sialic acids	1483:1503	terminal sialic acids attached to N-linked membrane glycoproteins on B cells	1483:1558	We can conclude that terminal sialic acids attached to N-linked membrane glycoproteins on B cells are used for virus attachment and are essential for successful virus infection.
32459612	7	26	attach	attached	1505:1512	arg1	glycoproteins					1535:1547	N-linked membrane glycoproteins	1517:1547	N-linked membrane glycoproteins	1517:1547	We can conclude that terminal sialic acids attached to N-linked membrane glycoproteins on B cells are used for virus attachment and are essential for successful virus infection.
32459612	4	27	theme	attachment	765:774	arg1	receptor					776:783	attachment receptor	765:783	attachment receptor	765:783	Pre-incubation of cells with Maackia amurensis lectin or Sambucus nigra agglutinin resulted in virus reduction, suggesting that TAdV-3 uses both α2,3-linked and α2,6-linked sialic acids as attachment receptor.
32459612	2	28	theme	structural	355:364	arg1	components					366:375	the structural components	351:375	the structural components of TAdV-3 receptor(s) on RP19, B lymphoblastoid cells	351:429	In the present study, we investigated the role of sialic acid in TAdV-3 entry and characterized the structural components of TAdV-3 receptor(s) on RP19, B lymphoblastoid cells.
32459612	0	29	link	N-linked	57:64	arg1	glycoproteins					66:78	N-linked glycoproteins	57:78	N-linked glycoproteins	57:78	Turkey adenovirus 3, a siadenovirus, uses sialic acid on N-linked glycoproteins as a cellular receptor.
32459612	0	30	theme	sialic	42:47	arg1	receptor					94:101	a cellular receptor	83:101	a cellular receptor	83:101	Turkey adenovirus 3, a siadenovirus, uses sialic acid on N-linked glycoproteins as a cellular receptor.
32459612	0	30	theme	sialic	42:47	arg1	acid					49:52	sialic acid	42:52	sialic acid on N-linked glycoproteins	42:78	Turkey adenovirus 3, a siadenovirus, uses sialic acid on N-linked glycoproteins as a cellular receptor.
32459612	7	31	theme	terminal	1483:1490	arg1	acids					1499:1503	terminal sialic acids	1483:1503	terminal sialic acids attached to N-linked membrane glycoproteins on B cells	1483:1558	We can conclude that terminal sialic acids attached to N-linked membrane glycoproteins on B cells are used for virus attachment and are essential for successful virus infection.
32459612	5	32	theme	N-linked	1041:1048	arg1	carbohydrates					1069:1081	N-linked, but not O-linked, carbohydrates	1041:1081	N-linked, but not O-linked, carbohydrates	1041:1081	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	5	32	theme	N-linked	1041:1048	arg1	part					1087:1090	part	1087:1090	part of the sialylated receptor	1087:1117	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	4	33	with	Pre-incubation	576:589	arg1	lectin					623:628	Maackia amurensis lectin	605:628	Maackia amurensis lectin	605:628	Pre-incubation of cells with Maackia amurensis lectin or Sambucus nigra agglutinin resulted in virus reduction, suggesting that TAdV-3 uses both α2,3-linked and α2,6-linked sialic acids as attachment receptor.
32459612	4	33	with	Pre-incubation	576:589	arg1	agglutinin					648:657	Sambucus nigra agglutinin	633:657	Sambucus nigra agglutinin	633:657	Pre-incubation of cells with Maackia amurensis lectin or Sambucus nigra agglutinin resulted in virus reduction, suggesting that TAdV-3 uses both α2,3-linked and α2,6-linked sialic acids as attachment receptor.
32459612	4	34	theme	α2,6-linked	737:747	arg1	acids					756:760	both α2,3-linked and α2,6-linked sialic acids	716:760	both α2,3-linked and α2,6-linked sialic acids	716:760	Pre-incubation of cells with Maackia amurensis lectin or Sambucus nigra agglutinin resulted in virus reduction, suggesting that TAdV-3 uses both α2,3-linked and α2,6-linked sialic acids as attachment receptor.
32459612	6	35	theme	glycolipid	1354:1363	arg1	biosynthesis					1365:1376	glycolipid biosynthesis	1354:1376	glycolipid biosynthesis	1354:1376	Furthermore, our data, in conjunction with previous findings, implies that the secondary receptor for TAdV-3 is a protein molecule since the inhibition of glycolipid biosynthesis did not affect the virus infection, which was rather reduced by protease treatment.
32459612	2	36	theme	sialic	305:310	arg1	acid					312:315	sialic acid	305:315	sialic acid	305:315	In the present study, we investigated the role of sialic acid in TAdV-3 entry and characterized the structural components of TAdV-3 receptor(s) on RP19, B lymphoblastoid cells.
32459612	7	37	gly	glycoproteins	1535:1547	arg1	glycoproteins					1535:1547	N-linked membrane glycoproteins	1517:1547	N-linked membrane glycoproteins	1517:1547	We can conclude that terminal sialic acids attached to N-linked membrane glycoproteins on B cells are used for virus attachment and are essential for successful virus infection.
32459612	6	38	theme	previous	1242:1249	arg1	findings					1251:1258	previous findings	1242:1258	previous findings	1242:1258	Furthermore, our data, in conjunction with previous findings, implies that the secondary receptor for TAdV-3 is a protein molecule since the inhibition of glycolipid biosynthesis did not affect the virus infection, which was rather reduced by protease treatment.
32459612	3	39	theme	sialic	460:465	arg1	acids					467:471	the cell-surface sialic acids	443:471	the cell-surface sialic acids	443:471	Removal of the cell-surface sialic acids by neuraminidases or blocking of sialic acids by wheat germ agglutinin lectin reduced virus infection.
32459612	5	40	theme	RP19	814:817	arg1	cells					819:823	RP19 cells	814:823	RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide)	814:1024	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	4	41	theme	cells	594:598	arg1	Pre-incubation					576:589	Pre-incubation	576:589	Pre-incubation of cells with Maackia amurensis lectin or Sambucus nigra agglutinin	576:657	Pre-incubation of cells with Maackia amurensis lectin or Sambucus nigra agglutinin resulted in virus reduction, suggesting that TAdV-3 uses both α2,3-linked and α2,6-linked sialic acids as attachment receptor.
32459612	1	42	theme	causative	140:148	arg1	agent					150:154	the causative agent	136:154	the causative agent of an immune-mediated disease in turkeys, haemorrhagic enteritis	136:219	Turkey adenovirus 3 (TAdV-3) is the causative agent of an immune-mediated disease in turkeys, haemorrhagic enteritis, through targeting B lymphocytes.
32459612	1	42	theme	causative	140:148	arg1	adenovirus					111:120	Turkey adenovirus 3	104:122	Turkey adenovirus 3 (TAdV-3)	104:131	Turkey adenovirus 3 (TAdV-3) is the causative agent of an immune-mediated disease in turkeys, haemorrhagic enteritis, through targeting B lymphocytes.
32459612	7	43	theme	successful	1612:1621	arg1	infection					1629:1637	successful virus infection	1612:1637	successful virus infection	1612:1637	We can conclude that terminal sialic acids attached to N-linked membrane glycoproteins on B cells are used for virus attachment and are essential for successful virus infection.
32459612	4	44	theme	Maackia	605:611	arg1	lectin					623:628	Maackia amurensis lectin	605:628	Maackia amurensis lectin	605:628	Pre-incubation of cells with Maackia amurensis lectin or Sambucus nigra agglutinin resulted in virus reduction, suggesting that TAdV-3 uses both α2,3-linked and α2,6-linked sialic acids as attachment receptor.
32459612	6	45	theme	protein	1313:1319	arg1	receptor					1288:1295	the secondary receptor	1274:1295	the secondary receptor for TAdV-3	1274:1306	Furthermore, our data, in conjunction with previous findings, implies that the secondary receptor for TAdV-3 is a protein molecule since the inhibition of glycolipid biosynthesis did not affect the virus infection, which was rather reduced by protease treatment.
32459612	6	45	theme	protein	1313:1319	arg1	molecule					1321:1328	a protein molecule	1311:1328	a protein molecule since the inhibition of glycolipid biosynthesis did not affect the virus infection, which was rather reduced by protease treatment	1311:1459	Furthermore, our data, in conjunction with previous findings, implies that the secondary receptor for TAdV-3 is a protein molecule since the inhibition of glycolipid biosynthesis did not affect the virus infection, which was rather reduced by protease treatment.
32459612	2	46	theme	present	262:268	arg1	study					270:274	the present study	258:274	the present study	258:274	In the present study, we investigated the role of sialic acid in TAdV-3 entry and characterized the structural components of TAdV-3 receptor(s) on RP19, B lymphoblastoid cells.
32459612	5	47	theme	Virus	786:790	arg1	data					804:807	Virus infectivity data	786:807	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide)	786:1024	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	1	48	from	enteritis	211:219	arg1	agent					150:154	the causative agent	136:154	the causative agent of an immune-mediated disease in turkeys, haemorrhagic enteritis	136:219	Turkey adenovirus 3 (TAdV-3) is the causative agent of an immune-mediated disease in turkeys, haemorrhagic enteritis, through targeting B lymphocytes.
32459612	1	48	from	enteritis	211:219	arg1	adenovirus					111:120	Turkey adenovirus 3	104:122	Turkey adenovirus 3 (TAdV-3)	104:131	Turkey adenovirus 3 (TAdV-3) is the causative agent of an immune-mediated disease in turkeys, haemorrhagic enteritis, through targeting B lymphocytes.
32459612	3	49	theme	wheat	522:526	arg1	agglutinin					533:542	wheat germ agglutinin	522:542	wheat germ agglutinin lectin	522:549	Removal of the cell-surface sialic acids by neuraminidases or blocking of sialic acids by wheat germ agglutinin lectin reduced virus infection.
32459612	1	50	theme	immune-mediated	162:176	arg1	disease					178:184	an immune-mediated disease	159:184	an immune-mediated disease in turkeys, haemorrhagic enteritis	159:219	Turkey adenovirus 3 (TAdV-3) is the causative agent of an immune-mediated disease in turkeys, haemorrhagic enteritis, through targeting B lymphocytes.
32459612	5	51	link	N-linked	1041:1048	arg1	carbohydrates					1069:1081	N-linked, but not O-linked, carbohydrates	1041:1081	N-linked, but not O-linked, carbohydrates	1041:1081	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	5	51	link	N-linked	1041:1048	arg1	part					1087:1090	part	1087:1090	part of the sialylated receptor	1087:1117	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	0	52	from	acid	49:52	arg1	glycoproteins					66:78	N-linked glycoproteins	57:78	N-linked glycoproteins	57:78	Turkey adenovirus 3, a siadenovirus, uses sialic acid on N-linked glycoproteins as a cellular receptor.
32459612	3	53	theme	acids	513:517	arg1	blocking					494:501	blocking	494:501	blocking of sialic acids by wheat germ agglutinin lectin	494:549	Removal of the cell-surface sialic acids by neuraminidases or blocking of sialic acids by wheat germ agglutinin lectin reduced virus infection.
32459612	3	53	theme	acids	513:517	arg1	neuraminidases					476:489	neuraminidases	476:489	neuraminidases	476:489	Removal of the cell-surface sialic acids by neuraminidases or blocking of sialic acids by wheat germ agglutinin lectin reduced virus infection.
32459612	1	54	from	disease	178:184	arg1	enteritis					211:219	turkeys, haemorrhagic enteritis	189:219	turkeys, haemorrhagic enteritis	189:219	Turkey adenovirus 3 (TAdV-3) is the causative agent of an immune-mediated disease in turkeys, haemorrhagic enteritis, through targeting B lymphocytes.
32459612	7	55	theme	virus	1573:1577	arg1	attachment					1579:1588	virus attachment	1573:1588	virus attachment	1573:1588	We can conclude that terminal sialic acids attached to N-linked membrane glycoproteins on B cells are used for virus attachment and are essential for successful virus infection.
32459612	6	56	theme	secondary	1278:1286	arg1	receptor					1288:1295	the secondary receptor	1274:1295	the secondary receptor for TAdV-3	1274:1306	Furthermore, our data, in conjunction with previous findings, implies that the secondary receptor for TAdV-3 is a protein molecule since the inhibition of glycolipid biosynthesis did not affect the virus infection, which was rather reduced by protease treatment.
32459612	6	56	theme	secondary	1278:1286	arg1	molecule					1321:1328	a protein molecule	1311:1328	a protein molecule since the inhibition of glycolipid biosynthesis did not affect the virus infection, which was rather reduced by protease treatment	1311:1459	Furthermore, our data, in conjunction with previous findings, implies that the secondary receptor for TAdV-3 is a protein molecule since the inhibition of glycolipid biosynthesis did not affect the virus infection, which was rather reduced by protease treatment.
32459612	1	57	theme	Turkey	104:109	arg1	adenovirus					111:120	Turkey adenovirus 3	104:122	Turkey adenovirus 3 (TAdV-3)	104:131	Turkey adenovirus 3 (TAdV-3) is the causative agent of an immune-mediated disease in turkeys, haemorrhagic enteritis, through targeting B lymphocytes.
32459612	1	57	theme	Turkey	104:109	arg1	TAdV-3					125:130	TAdV-3	125:130	TAdV-3	125:130	Turkey adenovirus 3 (TAdV-3) is the causative agent of an immune-mediated disease in turkeys, haemorrhagic enteritis, through targeting B lymphocytes.
32459612	1	57	theme	Turkey	104:109	arg1	agent					150:154	the causative agent	136:154	the causative agent of an immune-mediated disease in turkeys, haemorrhagic enteritis	136:219	Turkey adenovirus 3 (TAdV-3) is the causative agent of an immune-mediated disease in turkeys, haemorrhagic enteritis, through targeting B lymphocytes.
32459612	5	58	link	O-linked	1059:1066	arg1	carbohydrates					1069:1081	N-linked, but not O-linked, carbohydrates	1041:1081	N-linked, but not O-linked, carbohydrates	1041:1081	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	5	58	link	O-linked	1059:1066	arg1	part					1087:1090	part	1087:1090	part of the sialylated receptor	1087:1117	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	5	59	theme	O-linked	1059:1066	arg1	carbohydrates					1069:1081	N-linked, but not O-linked, carbohydrates	1041:1081	N-linked, but not O-linked, carbohydrates	1041:1081	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	5	59	theme	O-linked	1059:1066	arg1	part					1087:1090	part	1087:1090	part of the sialylated receptor	1087:1117	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	3	60	theme	agglutinin	533:542	arg1	lectin					544:549	wheat germ agglutinin lectin	522:549	wheat germ agglutinin lectin	522:549	Removal of the cell-surface sialic acids by neuraminidases or blocking of sialic acids by wheat germ agglutinin lectin reduced virus infection.
32459612	1	61	from	agent	150:154	arg1	enteritis					211:219	turkeys, haemorrhagic enteritis	189:219	turkeys, haemorrhagic enteritis	189:219	Turkey adenovirus 3 (TAdV-3) is the causative agent of an immune-mediated disease in turkeys, haemorrhagic enteritis, through targeting B lymphocytes.
32459612	5	62	theme	sodium	838:843	arg1	periodate					845:853	sodium periodate	838:853	sodium periodate	838:853	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	5	63	dep	inhibitors	902:911	arg1	inhibitors					902:911	metabolic inhibitors	892:911	metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide)	892:1024	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	5	63	dep	inhibitors	902:911	arg1	dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol					914:970	dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol	914:970	dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol	914:970	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	5	63	dep	inhibitors	902:911	arg1	N-acetyl-α-d-galactosaminide					996:1023	benzyl N-acetyl-α-d-galactosaminide	989:1023	benzyl N-acetyl-α-d-galactosaminide	989:1023	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	5	63	dep	inhibitors	902:911	arg1	tunicamycin					973:983	tunicamycin	973:983	tunicamycin	973:983	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	0	64	theme	N-linked	57:64	arg1	glycoproteins					66:78	N-linked glycoproteins	57:78	N-linked glycoproteins	57:78	Turkey adenovirus 3, a siadenovirus, uses sialic acid on N-linked glycoproteins as a cellular receptor.
32459612	5	65	from	cells	819:823	arg1	data					804:807	Virus infectivity data	786:807	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide)	786:1024	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	2	66	theme	B	408:408	arg1	cells					425:429	RP19, B lymphoblastoid cells	402:429	cells	425:429	In the present study, we investigated the role of sialic acid in TAdV-3 entry and characterized the structural components of TAdV-3 receptor(s) on RP19, B lymphoblastoid cells.
32459612	4	67	theme	Sambucus	633:640	arg1	agglutinin					648:657	Sambucus nigra agglutinin	633:657	Sambucus nigra agglutinin	633:657	Pre-incubation of cells with Maackia amurensis lectin or Sambucus nigra agglutinin resulted in virus reduction, suggesting that TAdV-3 uses both α2,3-linked and α2,6-linked sialic acids as attachment receptor.
32459612	7	68	theme	membrane	1526:1533	arg1	glycoproteins					1535:1547	N-linked membrane glycoproteins	1517:1547	N-linked membrane glycoproteins	1517:1547	We can conclude that terminal sialic acids attached to N-linked membrane glycoproteins on B cells are used for virus attachment and are essential for successful virus infection.
32459612	0	69	theme	cellular	85:92	arg1	receptor					94:101	a cellular receptor	83:101	a cellular receptor	83:101	Turkey adenovirus 3, a siadenovirus, uses sialic acid on N-linked glycoproteins as a cellular receptor.
32459612	0	69	theme	cellular	85:92	arg1	acid					49:52	sialic acid	42:52	sialic acid on N-linked glycoproteins	42:78	Turkey adenovirus 3, a siadenovirus, uses sialic acid on N-linked glycoproteins as a cellular receptor.
32459612	5	70	theme	receptor	1110:1117	arg1	carbohydrates					1069:1081	N-linked, but not O-linked, carbohydrates	1041:1081	N-linked, but not O-linked, carbohydrates	1041:1081	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	5	70	theme	receptor	1110:1117	arg1	part					1087:1090	part	1087:1090	part of the sialylated receptor	1087:1117	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	0	71	gly	glycoproteins	66:78	arg1	glycoproteins					66:78	N-linked glycoproteins	57:78	N-linked glycoproteins	57:78	Turkey adenovirus 3, a siadenovirus, uses sialic acid on N-linked glycoproteins as a cellular receptor.
32459612	5	72	dep	proteases	856:864	arg1	trypsin					867:873	trypsin	867:873	trypsin	867:873	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	5	72	dep	proteases	856:864	arg1	proteases					856:864	proteases	856:864	proteases (trypsin or bromelain)	856:887	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	5	72	dep	proteases	856:864	arg1	bromelain					878:886	bromelain	878:886	bromelain	878:886	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	2	73	theme	TAdV-3	320:325	arg1	entry					327:331	TAdV-3 entry	320:331	TAdV-3 entry	320:331	In the present study, we investigated the role of sialic acid in TAdV-3 entry and characterized the structural components of TAdV-3 receptor(s) on RP19, B lymphoblastoid cells.
32459612	5	74	theme	metabolic	892:900	arg1	dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol					914:970	dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol	914:970	dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol	914:970	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	5	74	theme	metabolic	892:900	arg1	inhibitors					902:911	metabolic inhibitors	892:911	metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide)	892:1024	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	5	74	theme	metabolic	892:900	arg1	N-acetyl-α-d-galactosaminide					996:1023	benzyl N-acetyl-α-d-galactosaminide	989:1023	benzyl N-acetyl-α-d-galactosaminide	989:1023	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	5	74	theme	metabolic	892:900	arg1	tunicamycin					973:983	tunicamycin	973:983	tunicamycin	973:983	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	2	75	theme	TAdV-3	380:385	arg1	s					396:396	TAdV-3 receptor(s)	380:397	TAdV-3 receptor(s)	380:397	In the present study, we investigated the role of sialic acid in TAdV-3 entry and characterized the structural components of TAdV-3 receptor(s) on RP19, B lymphoblastoid cells.
32459612	6	76	theme	virus	1397:1401	arg1	infection					1403:1411	the virus infection	1393:1411	the virus infection	1393:1411	Furthermore, our data, in conjunction with previous findings, implies that the secondary receptor for TAdV-3 is a protein molecule since the inhibition of glycolipid biosynthesis did not affect the virus infection, which was rather reduced by protease treatment.
32459612	7	77	theme	N-linked	1517:1524	arg1	glycoproteins					1535:1547	N-linked membrane glycoproteins	1517:1547	N-linked membrane glycoproteins	1517:1547	We can conclude that terminal sialic acids attached to N-linked membrane glycoproteins on B cells are used for virus attachment and are essential for successful virus infection.
32459612	4	78	link	α2,6-linked	737:747	arg1	acids					756:760	both α2,3-linked and α2,6-linked sialic acids	716:760	both α2,3-linked and α2,6-linked sialic acids	716:760	Pre-incubation of cells with Maackia amurensis lectin or Sambucus nigra agglutinin resulted in virus reduction, suggesting that TAdV-3 uses both α2,3-linked and α2,6-linked sialic acids as attachment receptor.
32459612	2	79	theme	RP19	402:405	arg1	cells					425:429	RP19, B lymphoblastoid cells	402:429	cells	425:429	In the present study, we investigated the role of sialic acid in TAdV-3 entry and characterized the structural components of TAdV-3 receptor(s) on RP19, B lymphoblastoid cells.
32459612	4	80	link	α2,3-linked	721:731	arg1	acids					756:760	both α2,3-linked and α2,6-linked sialic acids	716:760	both α2,3-linked and α2,6-linked sialic acids	716:760	Pre-incubation of cells with Maackia amurensis lectin or Sambucus nigra agglutinin resulted in virus reduction, suggesting that TAdV-3 uses both α2,3-linked and α2,6-linked sialic acids as attachment receptor.
32459612	5	81	theme	membrane	1150:1157	arg1	glycoprotein					1159:1170	a membrane glycoprotein	1148:1170	a membrane glycoprotein	1148:1170	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	7	82	theme	sialic	1492:1497	arg1	acids					1499:1503	terminal sialic acids	1483:1503	terminal sialic acids attached to N-linked membrane glycoproteins on B cells	1483:1558	We can conclude that terminal sialic acids attached to N-linked membrane glycoproteins on B cells are used for virus attachment and are essential for successful virus infection.
32459612	2	83	theme	acid	312:315	arg1	role					297:300	the role	293:300	the role of sialic acid in TAdV-3 entry	293:331	In the present study, we investigated the role of sialic acid in TAdV-3 entry and characterized the structural components of TAdV-3 receptor(s) on RP19, B lymphoblastoid cells.
32459612	1	84	theme	turkeys	189:195	arg1	enteritis					211:219	turkeys, haemorrhagic enteritis	189:219	turkeys, haemorrhagic enteritis	189:219	Turkey adenovirus 3 (TAdV-3) is the causative agent of an immune-mediated disease in turkeys, haemorrhagic enteritis, through targeting B lymphocytes.
32459612	5	85	theme	benzyl	989:994	arg1	inhibitors					902:911	metabolic inhibitors	892:911	metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide)	892:1024	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	5	85	theme	benzyl	989:994	arg1	N-acetyl-α-d-galactosaminide					996:1023	benzyl N-acetyl-α-d-galactosaminide	989:1023	benzyl N-acetyl-α-d-galactosaminide	989:1023	Virus infectivity data from RP19 cells treated with sodium periodate, proteases (trypsin or bromelain) or metabolic inhibitors (dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, tunicamycin, or benzyl N-acetyl-α-d-galactosaminide) indicated that N-linked, but not O-linked, carbohydrates are part of the sialylated receptor and they are likely based on a membrane glycoprotein, rather than a glycolipid.
32459612	6	86	theme	protease	1442:1449	arg1	treatment					1451:1459	protease treatment	1442:1459	protease treatment	1442:1459	Furthermore, our data, in conjunction with previous findings, implies that the secondary receptor for TAdV-3 is a protein molecule since the inhibition of glycolipid biosynthesis did not affect the virus infection, which was rather reduced by protease treatment.
32459612	4	87	theme	sialic	749:754	arg1	acids					756:760	both α2,3-linked and α2,6-linked sialic acids	716:760	both α2,3-linked and α2,6-linked sialic acids	716:760	Pre-incubation of cells with Maackia amurensis lectin or Sambucus nigra agglutinin resulted in virus reduction, suggesting that TAdV-3 uses both α2,3-linked and α2,6-linked sialic acids as attachment receptor.
32459612	3	88	theme	cell-surface	447:458	arg1	acids					467:471	the cell-surface sialic acids	443:471	the cell-surface sialic acids	443:471	Removal of the cell-surface sialic acids by neuraminidases or blocking of sialic acids by wheat germ agglutinin lectin reduced virus infection.
32459612	6	89	with	conjunction	1225:1235	arg1	findings					1251:1258	previous findings	1242:1258	previous findings	1242:1258	Furthermore, our data, in conjunction with previous findings, implies that the secondary receptor for TAdV-3 is a protein molecule since the inhibition of glycolipid biosynthesis did not affect the virus infection, which was rather reduced by protease treatment.
34951587	4	0	theme	pools	669:673	arg1	fidelity					633:640	fidelity	633:640	fidelity of glycosylation precursor pools	633:673	In contrast, GFAT1 knockout did not preclude the growth of human tumor xenografts in mice, suggesting that cancer cells can maintain fidelity of glycosylation precursor pools by scavenging nutrients from the tumor microenvironment.
34951587	5	1	theme	hyaluronic	746:755	arg1	HA					763:764	HA	763:764	HA	763:764	We found that hyaluronic acid (HA), an abundant carbohydrate polymer in pancreatic tumors composed of repeating N-acetyl-glucosamine (GlcNAc) and glucuronic acid sugars, can bypass GFAT1 to refuel the HBP via the GlcNAc salvage pathway.
34951587	5	1	theme	hyaluronic	746:755	arg1	acid					757:760	hyaluronic acid	746:760	hyaluronic acid (HA)	746:765	We found that hyaluronic acid (HA), an abundant carbohydrate polymer in pancreatic tumors composed of repeating N-acetyl-glucosamine (GlcNAc) and glucuronic acid sugars, can bypass GFAT1 to refuel the HBP via the GlcNAc salvage pathway.
34951587	5	1	theme	hyaluronic	746:755	arg1	polymer					793:799	an abundant carbohydrate polymer	768:799	an abundant carbohydrate polymer in pancreatic tumors composed of repeating N-acetyl-glucosamine (GlcNAc) and glucuronic acid sugars	768:899	We found that hyaluronic acid (HA), an abundant carbohydrate polymer in pancreatic tumors composed of repeating N-acetyl-glucosamine (GlcNAc) and glucuronic acid sugars, can bypass GFAT1 to refuel the HBP via the GlcNAc salvage pathway.
34951587	3	2	theme	cell	488:491	arg1	death					493:497	cell death	488:497	cell death	488:497	Here, we genetically ablated GFAT1 in human PDA cell lines, which completely blocked proliferation in vitro and led to cell death.
34951587	5	3	dep	GlcNAc	866:871	arg1	sugars					894:899	sugars	894:899	sugars	894:899	We found that hyaluronic acid (HA), an abundant carbohydrate polymer in pancreatic tumors composed of repeating N-acetyl-glucosamine (GlcNAc) and glucuronic acid sugars, can bypass GFAT1 to refuel the HBP via the GlcNAc salvage pathway.
34951587	1	4	theme	Rewired	53:59	arg1	metabolism					61:70	Rewired metabolism	53:70	Rewired metabolism	53:70	Rewired metabolism is a hallmark of pancreatic ductal adenocarcinomas (PDA).
34951587	1	4	theme	Rewired	53:59	arg1	hallmark					77:84	a hallmark	75:84	a hallmark of pancreatic ductal adenocarcinomas (PDA)	75:127	Rewired metabolism is a hallmark of pancreatic ductal adenocarcinomas (PDA).
34951587	2	5	theme	rate	288:291	arg1	activation					270:279	activation	270:279	activation of the rate limiting enzyme, glutamine-fructose 6-phosphate amidotransferase 1 (GFAT1)	270:366	Previously, we demonstrated that PDA cells enhance glycosylation precursor biogenesis through the hexosamine biosynthetic pathway (HBP) via activation of the rate limiting enzyme, glutamine-fructose 6-phosphate amidotransferase 1 (GFAT1).
34951587	4	6	theme	human	559:563	arg1	xenografts					571:580	human tumor xenografts	559:580	human tumor xenografts	559:580	In contrast, GFAT1 knockout did not preclude the growth of human tumor xenografts in mice, suggesting that cancer cells can maintain fidelity of glycosylation precursor pools by scavenging nutrients from the tumor microenvironment.
34951587	6	7	theme	PDA	1030:1032	arg1	metabolism					1034:1043	PDA metabolism	1030:1043	PDA metabolism	1030:1043	Together, these data show HA can serve as a nutrient fueling PDA metabolism beyond its previously appreciated structural and signaling roles.
34951587	5	8	theme	pancreatic	804:813	arg1	tumors					815:820	pancreatic tumors	804:820	pancreatic tumors composed of repeating N-acetyl-glucosamine (GlcNAc) and glucuronic acid sugars	804:899	We found that hyaluronic acid (HA), an abundant carbohydrate polymer in pancreatic tumors composed of repeating N-acetyl-glucosamine (GlcNAc) and glucuronic acid sugars, can bypass GFAT1 to refuel the HBP via the GlcNAc salvage pathway.
34951587	4	9	theme	precursor	659:667	arg1	pools					669:673	glycosylation precursor pools	645:673	glycosylation precursor pools	645:673	In contrast, GFAT1 knockout did not preclude the growth of human tumor xenografts in mice, suggesting that cancer cells can maintain fidelity of glycosylation precursor pools by scavenging nutrients from the tumor microenvironment.
34951587	4	10	theme	scavenging	678:687	arg1	nutrients					689:697	scavenging nutrients	678:697	scavenging nutrients from the tumor microenvironment	678:729	In contrast, GFAT1 knockout did not preclude the growth of human tumor xenografts in mice, suggesting that cancer cells can maintain fidelity of glycosylation precursor pools by scavenging nutrients from the tumor microenvironment.
34951587	4	11	from	microenvironment	714:729	arg1	nutrients					689:697	scavenging nutrients	678:697	scavenging nutrients from the tumor microenvironment	678:729	In contrast, GFAT1 knockout did not preclude the growth of human tumor xenografts in mice, suggesting that cancer cells can maintain fidelity of glycosylation precursor pools by scavenging nutrients from the tumor microenvironment.
34951587	4	12	theme	tumor	708:712	arg1	microenvironment					714:729	the tumor microenvironment	704:729	the tumor microenvironment	704:729	In contrast, GFAT1 knockout did not preclude the growth of human tumor xenografts in mice, suggesting that cancer cells can maintain fidelity of glycosylation precursor pools by scavenging nutrients from the tumor microenvironment.
34951587	3	13	theme	PDA	413:415	arg1	lines					422:426	human PDA cell lines	407:426	human PDA cell lines	407:426	Here, we genetically ablated GFAT1 in human PDA cell lines, which completely blocked proliferation in vitro and led to cell death.
34951587	0	14	theme	Hyaluronic	0:9	arg1	acid					11:14	Hyaluronic acid	0:14	Hyaluronic acid	0:14	Hyaluronic acid fuels pancreatic cancer cell growth.
34951587	3	15	theme	cell	417:420	arg1	lines					422:426	human PDA cell lines	407:426	human PDA cell lines	407:426	Here, we genetically ablated GFAT1 in human PDA cell lines, which completely blocked proliferation in vitro and led to cell death.
34951587	0	16	theme	pancreatic	22:31	arg1	growth					45:50	pancreatic cancer cell growth	22:50	pancreatic cancer cell growth	22:50	Hyaluronic acid fuels pancreatic cancer cell growth.
34951587	4	17	theme	knockout	519:526	arg1	GFAT1					513:517	GFAT1 knockout	513:526	GFAT1 knockout	513:526	In contrast, GFAT1 knockout did not preclude the growth of human tumor xenografts in mice, suggesting that cancer cells can maintain fidelity of glycosylation precursor pools by scavenging nutrients from the tumor microenvironment.
34951587	5	18	theme	glucuronic	878:887	arg1	acid					889:892	glucuronic acid	878:892	glucuronic acid	878:892	We found that hyaluronic acid (HA), an abundant carbohydrate polymer in pancreatic tumors composed of repeating N-acetyl-glucosamine (GlcNAc) and glucuronic acid sugars, can bypass GFAT1 to refuel the HBP via the GlcNAc salvage pathway.
34951587	1	19	theme	pancreatic	89:98	arg1	PDA					124:126	PDA	124:126	PDA	124:126	Rewired metabolism is a hallmark of pancreatic ductal adenocarcinomas (PDA).
34951587	1	19	theme	pancreatic	89:98	arg1	adenocarcinomas					107:121	pancreatic ductal adenocarcinomas	89:121	pancreatic ductal adenocarcinomas (PDA)	89:127	Rewired metabolism is a hallmark of pancreatic ductal adenocarcinomas (PDA).
34951587	5	20	theme	carbohydrate	780:791	arg1	polymer					793:799	an abundant carbohydrate polymer	768:799	an abundant carbohydrate polymer in pancreatic tumors composed of repeating N-acetyl-glucosamine (GlcNAc) and glucuronic acid sugars	768:899	We found that hyaluronic acid (HA), an abundant carbohydrate polymer in pancreatic tumors composed of repeating N-acetyl-glucosamine (GlcNAc) and glucuronic acid sugars, can bypass GFAT1 to refuel the HBP via the GlcNAc salvage pathway.
34951587	5	20	theme	carbohydrate	780:791	arg1	acid					757:760	hyaluronic acid	746:760	hyaluronic acid (HA)	746:765	We found that hyaluronic acid (HA), an abundant carbohydrate polymer in pancreatic tumors composed of repeating N-acetyl-glucosamine (GlcNAc) and glucuronic acid sugars, can bypass GFAT1 to refuel the HBP via the GlcNAc salvage pathway.
34951587	1	21	theme	ductal	100:105	arg1	PDA					124:126	PDA	124:126	PDA	124:126	Rewired metabolism is a hallmark of pancreatic ductal adenocarcinomas (PDA).
34951587	1	21	theme	ductal	100:105	arg1	adenocarcinomas					107:121	pancreatic ductal adenocarcinomas	89:121	pancreatic ductal adenocarcinomas (PDA)	89:127	Rewired metabolism is a hallmark of pancreatic ductal adenocarcinomas (PDA).
34951587	0	22	theme	cell	40:43	arg1	growth					45:50	pancreatic cancer cell growth	22:50	pancreatic cancer cell growth	22:50	Hyaluronic acid fuels pancreatic cancer cell growth.
34951587	2	23	theme	PDA	163:165	arg1	cells					167:171	PDA cells	163:171	PDA cells	163:171	Previously, we demonstrated that PDA cells enhance glycosylation precursor biogenesis through the hexosamine biosynthetic pathway (HBP) via activation of the rate limiting enzyme, glutamine-fructose 6-phosphate amidotransferase 1 (GFAT1).
34951587	2	24	theme	biosynthetic	239:250	arg1	HBP					261:263	HBP	261:263	HBP	261:263	Previously, we demonstrated that PDA cells enhance glycosylation precursor biogenesis through the hexosamine biosynthetic pathway (HBP) via activation of the rate limiting enzyme, glutamine-fructose 6-phosphate amidotransferase 1 (GFAT1).
34951587	2	24	theme	biosynthetic	239:250	arg1	pathway					252:258	the hexosamine biosynthetic pathway	224:258	the hexosamine biosynthetic pathway (HBP)	224:264	Previously, we demonstrated that PDA cells enhance glycosylation precursor biogenesis through the hexosamine biosynthetic pathway (HBP) via activation of the rate limiting enzyme, glutamine-fructose 6-phosphate amidotransferase 1 (GFAT1).
34951587	5	25	theme	N-acetyl-glucosamine	844:863	arg1	GlcNAc					866:871	N-acetyl-glucosamine (GlcNAc)	844:872	N-acetyl-glucosamine (GlcNAc)	844:872	We found that hyaluronic acid (HA), an abundant carbohydrate polymer in pancreatic tumors composed of repeating N-acetyl-glucosamine (GlcNAc) and glucuronic acid sugars, can bypass GFAT1 to refuel the HBP via the GlcNAc salvage pathway.
34951587	4	26	theme	cancer	607:612	arg1	cells					614:618	cancer cells	607:618	cancer cells	607:618	In contrast, GFAT1 knockout did not preclude the growth of human tumor xenografts in mice, suggesting that cancer cells can maintain fidelity of glycosylation precursor pools by scavenging nutrients from the tumor microenvironment.
34951587	0	27	theme	cancer	33:38	arg1	growth					45:50	pancreatic cancer cell growth	22:50	pancreatic cancer cell growth	22:50	Hyaluronic acid fuels pancreatic cancer cell growth.
34951587	3	28	theme	human	407:411	arg1	lines					422:426	human PDA cell lines	407:426	human PDA cell lines	407:426	Here, we genetically ablated GFAT1 in human PDA cell lines, which completely blocked proliferation in vitro and led to cell death.
34951587	2	29	theme	hexosamine	228:237	arg1	HBP					261:263	HBP	261:263	HBP	261:263	Previously, we demonstrated that PDA cells enhance glycosylation precursor biogenesis through the hexosamine biosynthetic pathway (HBP) via activation of the rate limiting enzyme, glutamine-fructose 6-phosphate amidotransferase 1 (GFAT1).
34951587	2	29	theme	hexosamine	228:237	arg1	pathway					252:258	the hexosamine biosynthetic pathway	224:258	the hexosamine biosynthetic pathway (HBP)	224:264	Previously, we demonstrated that PDA cells enhance glycosylation precursor biogenesis through the hexosamine biosynthetic pathway (HBP) via activation of the rate limiting enzyme, glutamine-fructose 6-phosphate amidotransferase 1 (GFAT1).
34951587	5	30	theme	abundant	771:778	arg1	polymer					793:799	an abundant carbohydrate polymer	768:799	an abundant carbohydrate polymer in pancreatic tumors composed of repeating N-acetyl-glucosamine (GlcNAc) and glucuronic acid sugars	768:899	We found that hyaluronic acid (HA), an abundant carbohydrate polymer in pancreatic tumors composed of repeating N-acetyl-glucosamine (GlcNAc) and glucuronic acid sugars, can bypass GFAT1 to refuel the HBP via the GlcNAc salvage pathway.
34951587	5	30	theme	abundant	771:778	arg1	acid					757:760	hyaluronic acid	746:760	hyaluronic acid (HA)	746:765	We found that hyaluronic acid (HA), an abundant carbohydrate polymer in pancreatic tumors composed of repeating N-acetyl-glucosamine (GlcNAc) and glucuronic acid sugars, can bypass GFAT1 to refuel the HBP via the GlcNAc salvage pathway.
34951587	1	31	theme	adenocarcinomas	107:121	arg1	metabolism					61:70	Rewired metabolism	53:70	Rewired metabolism	53:70	Rewired metabolism is a hallmark of pancreatic ductal adenocarcinomas (PDA).
34951587	1	31	theme	adenocarcinomas	107:121	arg1	hallmark					77:84	a hallmark	75:84	a hallmark of pancreatic ductal adenocarcinomas (PDA)	75:127	Rewired metabolism is a hallmark of pancreatic ductal adenocarcinomas (PDA).
34951587	5	32	from	polymer	793:799	arg1	tumors					815:820	pancreatic tumors	804:820	pancreatic tumors composed of repeating N-acetyl-glucosamine (GlcNAc) and glucuronic acid sugars	804:899	We found that hyaluronic acid (HA), an abundant carbohydrate polymer in pancreatic tumors composed of repeating N-acetyl-glucosamine (GlcNAc) and glucuronic acid sugars, can bypass GFAT1 to refuel the HBP via the GlcNAc salvage pathway.
34951587	6	33	theme	appreciated	1067:1077	arg1	roles					1104:1108	its previously appreciated structural and signaling roles	1052:1108	its previously appreciated structural and signaling roles	1052:1108	Together, these data show HA can serve as a nutrient fueling PDA metabolism beyond its previously appreciated structural and signaling roles.
34951587	2	34	theme	precursor	195:203	arg1	biogenesis					205:214	glycosylation precursor biogenesis	181:214	glycosylation precursor biogenesis	181:214	Previously, we demonstrated that PDA cells enhance glycosylation precursor biogenesis through the hexosamine biosynthetic pathway (HBP) via activation of the rate limiting enzyme, glutamine-fructose 6-phosphate amidotransferase 1 (GFAT1).
34951587	2	35	theme	6-phosphate	329:339	arg1	amidotransferase					341:356	glutamine-fructose 6-phosphate amidotransferase 1	310:358	glutamine-fructose 6-phosphate amidotransferase 1 (GFAT1)	310:366	Previously, we demonstrated that PDA cells enhance glycosylation precursor biogenesis through the hexosamine biosynthetic pathway (HBP) via activation of the rate limiting enzyme, glutamine-fructose 6-phosphate amidotransferase 1 (GFAT1).
34951587	2	35	theme	6-phosphate	329:339	arg1	GFAT1					361:365	GFAT1	361:365	GFAT1	361:365	Previously, we demonstrated that PDA cells enhance glycosylation precursor biogenesis through the hexosamine biosynthetic pathway (HBP) via activation of the rate limiting enzyme, glutamine-fructose 6-phosphate amidotransferase 1 (GFAT1).
34951587	2	35	theme	6-phosphate	329:339	arg1	rate					288:291	the rate	284:291	the rate limiting enzyme	284:307	Previously, we demonstrated that PDA cells enhance glycosylation precursor biogenesis through the hexosamine biosynthetic pathway (HBP) via activation of the rate limiting enzyme, glutamine-fructose 6-phosphate amidotransferase 1 (GFAT1).
34951587	6	36	theme	signaling	1094:1102	arg1	roles					1104:1108	its previously appreciated structural and signaling roles	1052:1108	its previously appreciated structural and signaling roles	1052:1108	Together, these data show HA can serve as a nutrient fueling PDA metabolism beyond its previously appreciated structural and signaling roles.
34951587	4	37	theme	xenografts	571:580	arg1	growth					549:554	the growth	545:554	the growth of human tumor xenografts	545:580	In contrast, GFAT1 knockout did not preclude the growth of human tumor xenografts in mice, suggesting that cancer cells can maintain fidelity of glycosylation precursor pools by scavenging nutrients from the tumor microenvironment.
34951587	2	38	theme	glycosylation	181:193	arg1	biogenesis					205:214	glycosylation precursor biogenesis	181:214	glycosylation precursor biogenesis	181:214	Previously, we demonstrated that PDA cells enhance glycosylation precursor biogenesis through the hexosamine biosynthetic pathway (HBP) via activation of the rate limiting enzyme, glutamine-fructose 6-phosphate amidotransferase 1 (GFAT1).
34951587	5	39	theme	GlcNAc	945:950	arg1	pathway					960:966	the GlcNAc salvage pathway	941:966	the GlcNAc salvage pathway	941:966	We found that hyaluronic acid (HA), an abundant carbohydrate polymer in pancreatic tumors composed of repeating N-acetyl-glucosamine (GlcNAc) and glucuronic acid sugars, can bypass GFAT1 to refuel the HBP via the GlcNAc salvage pathway.
34951587	2	40	theme	glutamine-fructose	310:327	arg1	amidotransferase					341:356	glutamine-fructose 6-phosphate amidotransferase 1	310:358	glutamine-fructose 6-phosphate amidotransferase 1 (GFAT1)	310:366	Previously, we demonstrated that PDA cells enhance glycosylation precursor biogenesis through the hexosamine biosynthetic pathway (HBP) via activation of the rate limiting enzyme, glutamine-fructose 6-phosphate amidotransferase 1 (GFAT1).
34951587	2	40	theme	glutamine-fructose	310:327	arg1	GFAT1					361:365	GFAT1	361:365	GFAT1	361:365	Previously, we demonstrated that PDA cells enhance glycosylation precursor biogenesis through the hexosamine biosynthetic pathway (HBP) via activation of the rate limiting enzyme, glutamine-fructose 6-phosphate amidotransferase 1 (GFAT1).
34951587	2	40	theme	glutamine-fructose	310:327	arg1	rate					288:291	the rate	284:291	the rate limiting enzyme	284:307	Previously, we demonstrated that PDA cells enhance glycosylation precursor biogenesis through the hexosamine biosynthetic pathway (HBP) via activation of the rate limiting enzyme, glutamine-fructose 6-phosphate amidotransferase 1 (GFAT1).
34951587	6	41	theme	structural	1079:1088	arg1	roles					1104:1108	its previously appreciated structural and signaling roles	1052:1108	its previously appreciated structural and signaling roles	1052:1108	Together, these data show HA can serve as a nutrient fueling PDA metabolism beyond its previously appreciated structural and signaling roles.
34951587	4	42	theme	tumor	565:569	arg1	xenografts					571:580	human tumor xenografts	559:580	human tumor xenografts	559:580	In contrast, GFAT1 knockout did not preclude the growth of human tumor xenografts in mice, suggesting that cancer cells can maintain fidelity of glycosylation precursor pools by scavenging nutrients from the tumor microenvironment.
34951587	5	43	theme	salvage	952:958	arg1	pathway					960:966	the GlcNAc salvage pathway	941:966	the GlcNAc salvage pathway	941:966	We found that hyaluronic acid (HA), an abundant carbohydrate polymer in pancreatic tumors composed of repeating N-acetyl-glucosamine (GlcNAc) and glucuronic acid sugars, can bypass GFAT1 to refuel the HBP via the GlcNAc salvage pathway.
34951587	4	44	theme	glycosylation	645:657	arg1	pools					669:673	glycosylation precursor pools	645:673	glycosylation precursor pools	645:673	In contrast, GFAT1 knockout did not preclude the growth of human tumor xenografts in mice, suggesting that cancer cells can maintain fidelity of glycosylation precursor pools by scavenging nutrients from the tumor microenvironment.
34552927	8	0	theme	new	1243:1245	arg1	insight					1247:1253	new insight	1243:1253	new insight into GAG profiles in various human diseases including cancerous tumors as well as neurodegenerative disease	1243:1361	These findings may provide new insight into GAG profiles in various human diseases including cancerous tumors as well as neurodegenerative disease using GlycoMaple analysis.
34552927	7	1	theme	versican	1118:1125	arg1	levels					1071:1076	the expression levels	1056:1076	the expression levels of the chondroitin sulfate-proteoglycan versican and the dermatan sulfate-proteoglycan decorin	1056:1171	In addition, the expression levels of the chondroitin sulfate-proteoglycan versican and the dermatan sulfate-proteoglycan decorin were up- and down-regulated, respectively.
34552927	1	2	theme	heparan	160:166	arg1	sulfate					168:174	heparan sulfate	160:174	heparan sulfate	160:174	Glycosaminoglycans (GAGs) including chondroitin sulfate, dermatan sulfate, heparan sulfate, and keratan sulfate, except for hyaluronan that is a free polysaccharide, are covalently attached to core proteins to form proteoglycans.
34552927	5	3	theme	chondroitin	746:756	arg1	biosynthesis					730:741	biosynthesis	730:741	biosynthesis of chondroitin and dermatan sulfate	730:777	In brain and pancreatic tumors, the pathways for biosynthesis of chondroitin and dermatan sulfate were predicted to be upregulated.
34552927	6	4	theme	dermatan	891:898	arg1	biosynthesis					859:870	biosynthesis	859:870	biosynthesis of chondroitin and dermatan sulfate	859:906	In breast cancerous tissues, the pathways for biosynthesis of chondroitin and dermatan sulfate were predicted to be up- and down-regulated, respectively, which are consistent with biochemical findings published in the literature.
34552927	3	5	theme	glycan	498:503	arg1	structures					505:514	glycan structures	498:514	glycan structures based on gene expression profiles	498:548	We recently developed a comprehensive glycosylation mapping tool, GlycoMaple, for visualization and estimation of glycan structures based on gene expression profiles.
34552927	7	6	from	addition	1046:1053	arg1	up-					1178:1180	up-	1178:1180	up-	1178:1180	In addition, the expression levels of the chondroitin sulfate-proteoglycan versican and the dermatan sulfate-proteoglycan decorin were up- and down-regulated, respectively.
34552927	7	7	theme	chondroitin	1085:1095	arg1	versican					1118:1125	the chondroitin sulfate-proteoglycan versican	1081:1125	the chondroitin sulfate-proteoglycan versican	1081:1125	In addition, the expression levels of the chondroitin sulfate-proteoglycan versican and the dermatan sulfate-proteoglycan decorin were up- and down-regulated, respectively.
34552927	3	8	theme	expression	530:539	arg1	profiles					541:548	gene expression profiles	525:548	gene expression profiles	525:548	We recently developed a comprehensive glycosylation mapping tool, GlycoMaple, for visualization and estimation of glycan structures based on gene expression profiles.
34552927	6	9	theme	chondroitin	875:885	arg1	biosynthesis					859:870	biosynthesis	859:870	biosynthesis of chondroitin and dermatan sulfate	859:906	In breast cancerous tissues, the pathways for biosynthesis of chondroitin and dermatan sulfate were predicted to be up- and down-regulated, respectively, which are consistent with biochemical findings published in the literature.
34552927	7	10	theme	sulfate-proteoglycan	1097:1116	arg1	versican					1118:1125	the chondroitin sulfate-proteoglycan versican	1081:1125	the chondroitin sulfate-proteoglycan versican	1081:1125	In addition, the expression levels of the chondroitin sulfate-proteoglycan versican and the dermatan sulfate-proteoglycan decorin were up- and down-regulated, respectively.
34552927	1	11	attach	attached	266:273	arg2	Glycosaminoglycans					85:102	Glycosaminoglycans	85:102	Glycosaminoglycans (GAGs)	85:109	Glycosaminoglycans (GAGs) including chondroitin sulfate, dermatan sulfate, heparan sulfate, and keratan sulfate, except for hyaluronan that is a free polysaccharide, are covalently attached to core proteins to form proteoglycans.
34552927	1	11	attach	attached	266:273	arg2	sulfate					133:139	chondroitin sulfate	121:139	chondroitin sulfate	121:139	Glycosaminoglycans (GAGs) including chondroitin sulfate, dermatan sulfate, heparan sulfate, and keratan sulfate, except for hyaluronan that is a free polysaccharide, are covalently attached to core proteins to form proteoglycans.
34552927	1	11	attach	attached	266:273	arg2	GAGs					105:108	GAGs	105:108	GAGs	105:108	Glycosaminoglycans (GAGs) including chondroitin sulfate, dermatan sulfate, heparan sulfate, and keratan sulfate, except for hyaluronan that is a free polysaccharide, are covalently attached to core proteins to form proteoglycans.
34552927	1	11	attach	attached	266:273	arg1	proteins					283:290	core proteins	278:290	core proteins	278:290	Glycosaminoglycans (GAGs) including chondroitin sulfate, dermatan sulfate, heparan sulfate, and keratan sulfate, except for hyaluronan that is a free polysaccharide, are covalently attached to core proteins to form proteoglycans.
34552927	1	11	attach	attached	266:273	arg2	sulfate					189:195	keratan sulfate	181:195	keratan sulfate	181:195	Glycosaminoglycans (GAGs) including chondroitin sulfate, dermatan sulfate, heparan sulfate, and keratan sulfate, except for hyaluronan that is a free polysaccharide, are covalently attached to core proteins to form proteoglycans.
34552927	1	11	attach	attached	266:273	arg2	sulfate					151:157	dermatan sulfate	142:157	dermatan sulfate	142:157	Glycosaminoglycans (GAGs) including chondroitin sulfate, dermatan sulfate, heparan sulfate, and keratan sulfate, except for hyaluronan that is a free polysaccharide, are covalently attached to core proteins to form proteoglycans.
34552927	1	11	attach	attached	266:273	arg2	sulfate					168:174	heparan sulfate	160:174	heparan sulfate	160:174	Glycosaminoglycans (GAGs) including chondroitin sulfate, dermatan sulfate, heparan sulfate, and keratan sulfate, except for hyaluronan that is a free polysaccharide, are covalently attached to core proteins to form proteoglycans.
34552927	4	12	theme	expression	572:581	arg1	levels					583:588	the expression levels	568:588	the expression levels of GAG biosynthetic genes	568:614	Using this tool, the expression levels of GAG biosynthetic genes were analyzed in various human tissues as well as tumor tissues.
34552927	2	13	theme	gene	328:331	arg1	products					333:340	More than 50 gene products	315:340	More than 50 gene products	315:340	More than 50 gene products are involved in the biosynthesis of GAGs.
34552927	2	14	theme	GAGs	378:381	arg1	biosynthesis					362:373	the biosynthesis	358:373	the biosynthesis of GAGs	358:381	More than 50 gene products are involved in the biosynthesis of GAGs.
34552927	7	15	theme	dermatan	1135:1142	arg1	decorin					1165:1171	the dermatan sulfate-proteoglycan decorin	1131:1171	the dermatan sulfate-proteoglycan decorin	1131:1171	In addition, the expression levels of the chondroitin sulfate-proteoglycan versican and the dermatan sulfate-proteoglycan decorin were up- and down-regulated, respectively.
34552927	8	16	theme	cancerous	1309:1317	arg1	tumors					1319:1324	cancerous tumors	1309:1324	cancerous tumors	1309:1324	These findings may provide new insight into GAG profiles in various human diseases including cancerous tumors as well as neurodegenerative disease using GlycoMaple analysis.
34552927	3	17	theme	gene	525:528	arg1	profiles					541:548	gene expression profiles	525:548	gene expression profiles	525:548	We recently developed a comprehensive glycosylation mapping tool, GlycoMaple, for visualization and estimation of glycan structures based on gene expression profiles.
34552927	3	18	theme	mapping	436:442	arg1	GlycoMaple					450:459	GlycoMaple	450:459	GlycoMaple	450:459	We recently developed a comprehensive glycosylation mapping tool, GlycoMaple, for visualization and estimation of glycan structures based on gene expression profiles.
34552927	3	18	theme	mapping	436:442	arg1	tool					444:447	a comprehensive glycosylation mapping tool	406:447	a comprehensive glycosylation mapping tool	406:447	We recently developed a comprehensive glycosylation mapping tool, GlycoMaple, for visualization and estimation of glycan structures based on gene expression profiles.
34552927	7	19	theme	expression	1060:1069	arg1	levels					1071:1076	the expression levels	1056:1076	the expression levels of the chondroitin sulfate-proteoglycan versican and the dermatan sulfate-proteoglycan decorin	1056:1171	In addition, the expression levels of the chondroitin sulfate-proteoglycan versican and the dermatan sulfate-proteoglycan decorin were up- and down-regulated, respectively.
34552927	7	20	from	up-	1178:1180	arg1	addition					1046:1053	addition	1046:1053	addition	1046:1053	In addition, the expression levels of the chondroitin sulfate-proteoglycan versican and the dermatan sulfate-proteoglycan decorin were up- and down-regulated, respectively.
34552927	1	21	theme	keratan	181:187	arg1	sulfate					189:195	keratan sulfate	181:195	keratan sulfate	181:195	Glycosaminoglycans (GAGs) including chondroitin sulfate, dermatan sulfate, heparan sulfate, and keratan sulfate, except for hyaluronan that is a free polysaccharide, are covalently attached to core proteins to form proteoglycans.
34552927	0	22	theme	Novel	0:4	arg1	Insight					6:12	Novel Insight	0:12	Novel Insight Into Glycosaminoglycan Biosynthesis	0:48	Novel Insight Into Glycosaminoglycan Biosynthesis Based on Gene Expression Profiles.
34552927	7	23	theme	decorin	1165:1171	arg1	levels					1071:1076	the expression levels	1056:1076	the expression levels of the chondroitin sulfate-proteoglycan versican and the dermatan sulfate-proteoglycan decorin	1056:1171	In addition, the expression levels of the chondroitin sulfate-proteoglycan versican and the dermatan sulfate-proteoglycan decorin were up- and down-regulated, respectively.
34552927	3	24	theme	comprehensive	408:420	arg1	GlycoMaple					450:459	GlycoMaple	450:459	GlycoMaple	450:459	We recently developed a comprehensive glycosylation mapping tool, GlycoMaple, for visualization and estimation of glycan structures based on gene expression profiles.
34552927	3	24	theme	comprehensive	408:420	arg1	tool					444:447	a comprehensive glycosylation mapping tool	406:447	a comprehensive glycosylation mapping tool	406:447	We recently developed a comprehensive glycosylation mapping tool, GlycoMaple, for visualization and estimation of glycan structures based on gene expression profiles.
34552927	0	25	theme	Glycosaminoglycan	19:35	arg1	Biosynthesis					37:48	Glycosaminoglycan Biosynthesis	19:48	Glycosaminoglycan Biosynthesis	19:48	Novel Insight Into Glycosaminoglycan Biosynthesis Based on Gene Expression Profiles.
34552927	8	26	theme	various	1276:1282	arg1	tumors					1319:1324	cancerous tumors	1309:1324	cancerous tumors	1309:1324	These findings may provide new insight into GAG profiles in various human diseases including cancerous tumors as well as neurodegenerative disease using GlycoMaple analysis.
34552927	8	26	theme	various	1276:1282	arg1	diseases					1290:1297	various human diseases	1276:1297	various human diseases including cancerous tumors	1276:1324	These findings may provide new insight into GAG profiles in various human diseases including cancerous tumors as well as neurodegenerative disease using GlycoMaple analysis.
34552927	8	27	from	disease	1355:1361	arg1	tumors					1319:1324	cancerous tumors	1309:1324	cancerous tumors	1309:1324	These findings may provide new insight into GAG profiles in various human diseases including cancerous tumors as well as neurodegenerative disease using GlycoMaple analysis.
34552927	8	27	from	disease	1355:1361	arg1	diseases					1290:1297	various human diseases	1276:1297	various human diseases including cancerous tumors	1276:1324	These findings may provide new insight into GAG profiles in various human diseases including cancerous tumors as well as neurodegenerative disease using GlycoMaple analysis.
34552927	1	28	theme	core	278:281	arg1	proteins					283:290	core proteins	278:290	core proteins	278:290	Glycosaminoglycans (GAGs) including chondroitin sulfate, dermatan sulfate, heparan sulfate, and keratan sulfate, except for hyaluronan that is a free polysaccharide, are covalently attached to core proteins to form proteoglycans.
34552927	5	29	dep	chondroitin	746:756	arg1	sulfate					771:777	sulfate	771:777	sulfate	771:777	In brain and pancreatic tumors, the pathways for biosynthesis of chondroitin and dermatan sulfate were predicted to be upregulated.
34552927	8	30	theme	neurodegenerative	1337:1353	arg1	disease					1355:1361	neurodegenerative disease	1337:1361	GAG profiles in various human diseases including cancerous tumors as well as neurodegenerative disease	1260:1361	These findings may provide new insight into GAG profiles in various human diseases including cancerous tumors as well as neurodegenerative disease using GlycoMaple analysis.
34552927	3	31	theme	structures	505:514	arg1	estimation					484:493	estimation	484:493	estimation	484:493	We recently developed a comprehensive glycosylation mapping tool, GlycoMaple, for visualization and estimation of glycan structures based on gene expression profiles.
34552927	3	31	theme	structures	505:514	arg1	visualization					466:478	visualization	466:478	visualization	466:478	We recently developed a comprehensive glycosylation mapping tool, GlycoMaple, for visualization and estimation of glycan structures based on gene expression profiles.
34552927	7	32	theme	sulfate-proteoglycan	1144:1163	arg1	decorin					1165:1171	the dermatan sulfate-proteoglycan decorin	1131:1171	the dermatan sulfate-proteoglycan decorin	1131:1171	In addition, the expression levels of the chondroitin sulfate-proteoglycan versican and the dermatan sulfate-proteoglycan decorin were up- and down-regulated, respectively.
34552927	8	33	theme	GAG	1260:1262	arg1	profiles					1264:1271	GAG profiles	1260:1271	GAG profiles in various human diseases including cancerous tumors as well as neurodegenerative disease	1260:1361	These findings may provide new insight into GAG profiles in various human diseases including cancerous tumors as well as neurodegenerative disease using GlycoMaple analysis.
34552927	4	34	theme	various	633:639	arg1	tissues					647:653	various human tissues	633:653	various human tissues as well as tumor tissues	633:678	Using this tool, the expression levels of GAG biosynthetic genes were analyzed in various human tissues as well as tumor tissues.
34552927	3	35	theme	glycosylation	422:434	arg1	GlycoMaple					450:459	GlycoMaple	450:459	GlycoMaple	450:459	We recently developed a comprehensive glycosylation mapping tool, GlycoMaple, for visualization and estimation of glycan structures based on gene expression profiles.
34552927	3	35	theme	glycosylation	422:434	arg1	tool					444:447	a comprehensive glycosylation mapping tool	406:447	a comprehensive glycosylation mapping tool	406:447	We recently developed a comprehensive glycosylation mapping tool, GlycoMaple, for visualization and estimation of glycan structures based on gene expression profiles.
34552927	5	36	theme	dermatan	762:769	arg1	biosynthesis					730:741	biosynthesis	730:741	biosynthesis of chondroitin and dermatan sulfate	730:777	In brain and pancreatic tumors, the pathways for biosynthesis of chondroitin and dermatan sulfate were predicted to be upregulated.
34552927	6	37	theme	biochemical	993:1003	arg1	findings					1005:1012	biochemical findings	993:1012	biochemical findings published in the literature	993:1040	In breast cancerous tissues, the pathways for biosynthesis of chondroitin and dermatan sulfate were predicted to be up- and down-regulated, respectively, which are consistent with biochemical findings published in the literature.
34552927	8	38	from	profiles	1264:1271	arg1	tumors					1319:1324	cancerous tumors	1309:1324	cancerous tumors	1309:1324	These findings may provide new insight into GAG profiles in various human diseases including cancerous tumors as well as neurodegenerative disease using GlycoMaple analysis.
34552927	8	38	from	profiles	1264:1271	arg1	diseases					1290:1297	various human diseases	1276:1297	various human diseases including cancerous tumors	1276:1324	These findings may provide new insight into GAG profiles in various human diseases including cancerous tumors as well as neurodegenerative disease using GlycoMaple analysis.
34552927	8	39	theme	human	1284:1288	arg1	tumors					1319:1324	cancerous tumors	1309:1324	cancerous tumors	1309:1324	These findings may provide new insight into GAG profiles in various human diseases including cancerous tumors as well as neurodegenerative disease using GlycoMaple analysis.
34552927	8	39	theme	human	1284:1288	arg1	diseases					1290:1297	various human diseases	1276:1297	various human diseases including cancerous tumors	1276:1324	These findings may provide new insight into GAG profiles in various human diseases including cancerous tumors as well as neurodegenerative disease using GlycoMaple analysis.
34552927	0	40	theme	Gene	59:62	arg1	Profiles					75:82	Gene Expression Profiles	59:82	Gene Expression Profiles	59:82	Novel Insight Into Glycosaminoglycan Biosynthesis Based on Gene Expression Profiles.
34552927	1	41	theme	chondroitin	121:131	arg1	sulfate					133:139	chondroitin sulfate	121:139	chondroitin sulfate	121:139	Glycosaminoglycans (GAGs) including chondroitin sulfate, dermatan sulfate, heparan sulfate, and keratan sulfate, except for hyaluronan that is a free polysaccharide, are covalently attached to core proteins to form proteoglycans.
34552927	5	42	theme	pancreatic	694:703	arg1	tumors					705:710	pancreatic tumors	694:710	pancreatic tumors	694:710	In brain and pancreatic tumors, the pathways for biosynthesis of chondroitin and dermatan sulfate were predicted to be upregulated.
34552927	6	43	theme	cancerous	823:831	arg1	tissues					833:839	breast cancerous tissues	816:839	breast cancerous tissues	816:839	In breast cancerous tissues, the pathways for biosynthesis of chondroitin and dermatan sulfate were predicted to be up- and down-regulated, respectively, which are consistent with biochemical findings published in the literature.
34552927	6	44	theme	breast	816:821	arg1	tissues					833:839	breast cancerous tissues	816:839	breast cancerous tissues	816:839	In breast cancerous tissues, the pathways for biosynthesis of chondroitin and dermatan sulfate were predicted to be up- and down-regulated, respectively, which are consistent with biochemical findings published in the literature.
34552927	4	45	theme	GAG	593:595	arg1	genes					610:614	GAG biosynthetic genes	593:614	GAG biosynthetic genes	593:614	Using this tool, the expression levels of GAG biosynthetic genes were analyzed in various human tissues as well as tumor tissues.
34552927	8	46	theme	GlycoMaple	1369:1378	arg1	analysis					1380:1387	GlycoMaple analysis	1369:1387	GlycoMaple analysis	1369:1387	These findings may provide new insight into GAG profiles in various human diseases including cancerous tumors as well as neurodegenerative disease using GlycoMaple analysis.
34552927	1	47	theme	dermatan	142:149	arg1	sulfate					151:157	dermatan sulfate	142:157	dermatan sulfate	142:157	Glycosaminoglycans (GAGs) including chondroitin sulfate, dermatan sulfate, heparan sulfate, and keratan sulfate, except for hyaluronan that is a free polysaccharide, are covalently attached to core proteins to form proteoglycans.
34552927	6	48	dep	chondroitin	875:885	arg1	sulfate					900:906	sulfate	900:906	sulfate	900:906	In breast cancerous tissues, the pathways for biosynthesis of chondroitin and dermatan sulfate were predicted to be up- and down-regulated, respectively, which are consistent with biochemical findings published in the literature.
34552927	6	49	with	consistent	977:986	arg1	findings					1005:1012	biochemical findings	993:1012	biochemical findings published in the literature	993:1040	In breast cancerous tissues, the pathways for biosynthesis of chondroitin and dermatan sulfate were predicted to be up- and down-regulated, respectively, which are consistent with biochemical findings published in the literature.
34552927	4	50	theme	tumor	666:670	arg1	tissues					672:678	tumor tissues	666:678	various human tissues as well as tumor tissues	633:678	Using this tool, the expression levels of GAG biosynthetic genes were analyzed in various human tissues as well as tumor tissues.
34552927	4	51	theme	human	641:645	arg1	tissues					647:653	various human tissues	633:653	various human tissues as well as tumor tissues	633:678	Using this tool, the expression levels of GAG biosynthetic genes were analyzed in various human tissues as well as tumor tissues.
34552927	4	52	theme	genes	610:614	arg1	levels					583:588	the expression levels	568:588	the expression levels of GAG biosynthetic genes	568:614	Using this tool, the expression levels of GAG biosynthetic genes were analyzed in various human tissues as well as tumor tissues.
34552927	0	53	theme	Expression	64:73	arg1	Profiles					75:82	Gene Expression Profiles	59:82	Gene Expression Profiles	59:82	Novel Insight Into Glycosaminoglycan Biosynthesis Based on Gene Expression Profiles.
34552927	4	54	theme	biosynthetic	597:608	arg1	genes					610:614	GAG biosynthetic genes	593:614	GAG biosynthetic genes	593:614	Using this tool, the expression levels of GAG biosynthetic genes were analyzed in various human tissues as well as tumor tissues.
34552927	1	55	theme	free	230:233	arg1	polysaccharide					235:248	a free polysaccharide	228:248	a free polysaccharide	228:248	Glycosaminoglycans (GAGs) including chondroitin sulfate, dermatan sulfate, heparan sulfate, and keratan sulfate, except for hyaluronan that is a free polysaccharide, are covalently attached to core proteins to form proteoglycans.
34552927	1	55	theme	free	230:233	arg1	hyaluronan					209:218	hyaluronan	209:218	hyaluronan that is a free polysaccharide	209:248	Glycosaminoglycans (GAGs) including chondroitin sulfate, dermatan sulfate, heparan sulfate, and keratan sulfate, except for hyaluronan that is a free polysaccharide, are covalently attached to core proteins to form proteoglycans.
32272702	9	0	theme	Wistar	1495:1500	arg1	rats					1502:1505	Wistar rats	1495:1505	Wistar rats	1495:1505	The results of subdermal implantation after 30 days in Wistar rats showed that increasing the amount of RGEO resulted in greater resorption of the material, but also more significant inflammation of the tissue surrounding the materials.
32272702	4	1	theme	allergic	722:729	arg1	reactions					731:739	allergic reactions	722:739	allergic reactions	722:739	In this work, CS films and Ruta graveolens essential oil (RGEO) were incorporated to obtain porous and resorbable materials, which did not generate allergic reactions.
32272702	3	2	theme	abundant	476:483	arg1	polysaccharide					485:498	the second most abundant polysaccharide	460:498	the second most abundant polysaccharide in nature	460:508	Chitosan (CS) is a biopolymer derived from chitin, the second most abundant polysaccharide in nature, which has been highly used for cell regeneration applications.
32272702	3	2	theme	abundant	476:483	arg1	chitin					452:457	chitin	452:457	chitin	452:457	Chitosan (CS) is a biopolymer derived from chitin, the second most abundant polysaccharide in nature, which has been highly used for cell regeneration applications.
32272702	7	3	theme	RGEO	1275:1278	arg1	incorporation					1280:1292	the RGEO incorporation	1271:1292	the RGEO incorporation	1271:1292	In contrast, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analysis showed that the crystalline structure and percentage of CS were slightly affected by the RGEO incorporation.
32272702	6	4	theme	spectroscopy	1032:1043	arg1	analyses					1052:1059	Infrared spectroscopy (FTIR) analyses	1023:1059	Infrared spectroscopy (FTIR) analyses	1023:1059	Infrared spectroscopy (FTIR) analyses demonstrated the presence of RGEO.
32272702	3	5	theme	cell	542:545	arg1	applications					560:571	cell regeneration applications	542:571	cell regeneration applications	542:571	Chitosan (CS) is a biopolymer derived from chitin, the second most abundant polysaccharide in nature, which has been highly used for cell regeneration applications.
32272702	1	6	theme	tissues	204:210	arg1	replacement					176:186	the replacement	172:186	the replacement of deteriorated tissues (due to accidents and diseases)	172:242	The development of new biocompatible materials for application in the replacement of deteriorated tissues (due to accidents and diseases) has gained a lot of attention due to the high demand around the world.
32272702	9	7	theme	RGEO	1544:1547	arg1	RGEO					1544:1547	RGEO	1544:1547	RGEO	1544:1547	The results of subdermal implantation after 30 days in Wistar rats showed that increasing the amount of RGEO resulted in greater resorption of the material, but also more significant inflammation of the tissue surrounding the materials.
32272702	9	7	theme	RGEO	1544:1547	arg1	amount					1534:1539	the amount	1530:1539	the amount of RGEO	1530:1547	The results of subdermal implantation after 30 days in Wistar rats showed that increasing the amount of RGEO resulted in greater resorption of the material, but also more significant inflammation of the tissue surrounding the materials.
32272702	3	8	used	used	533:536	arg2	Chitosan					409:416	Chitosan	409:416	Chitosan (CS)	409:421	Chitosan (CS) is a biopolymer derived from chitin, the second most abundant polysaccharide in nature, which has been highly used for cell regeneration applications.
32272702	3	8	used	used	533:536	arg2	biopolymer					428:437	a biopolymer	426:437	a biopolymer	426:437	Chitosan (CS) is a biopolymer derived from chitin, the second most abundant polysaccharide in nature, which has been highly used for cell regeneration applications.
32272702	11	9	theme	Young	1886:1890	arg1	modulus					1894:1900	Young's modulus	1886:1900	Young's modulus for F3 and F4	1886:1914	However, mechanical properties demonstrated an understandable loss of tensile strength and Young's modulus for F3 and F4.
32272702	8	10	theme	saturation	1307:1316	arg1	phenomena					1318:1326	Interesting saturation phenomena	1295:1326	Interesting saturation phenomena	1295:1326	Interesting saturation phenomena were observed for mechanical and water permeability tests when RGEO was incorporated at higher than 0.5% (v/v).
32272702	7	11	theme	CS	1242:1243	arg1	structure					1214:1222	crystalline structure	1202:1222	crystalline structure	1202:1222	In contrast, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analysis showed that the crystalline structure and percentage of CS were slightly affected by the RGEO incorporation.
32272702	7	11	theme	CS	1242:1243	arg1	percentage					1228:1237	percentage	1228:1237	percentage	1228:1237	In contrast, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analysis showed that the crystalline structure and percentage of CS were slightly affected by the RGEO incorporation.
32272702	5	12	theme	RGEO	950:953	arg1	incorporation					955:967	the RGEO incorporation	946:967	the RGEO incorporation	946:967	An oil-free formulation (F1: CS) and three different formulations containing R. graveolens essential oil were prepared (F2: CS-RGEO 0.5%; F3: CS+RGEO 1.0%; and F4: CS+RGEO 1.5%) to evaluate the effect of the RGEO incorporation in the mechanical and thermal stability of the films.
32272702	11	13	theme	mechanical	1804:1813	arg1	properties					1815:1824	mechanical properties	1804:1824	mechanical properties	1804:1824	However, mechanical properties demonstrated an understandable loss of tensile strength and Young's modulus for F3 and F4.
32272702	5	14	theme	different	785:793	arg1	formulations					795:806	three different formulations	779:806	three different formulations containing R. graveolens essential oil	779:845	An oil-free formulation (F1: CS) and three different formulations containing R. graveolens essential oil were prepared (F2: CS-RGEO 0.5%; F3: CS+RGEO 1.0%; and F4: CS+RGEO 1.5%) to evaluate the effect of the RGEO incorporation in the mechanical and thermal stability of the films.
32272702	13	15	dep	in	2207:2208	arg1	vivo					2210:2213	vivo	2210:2213	vivo	2210:2213	The cytotoxicity analysis of the CS+RGEO compositions by the hemolysis technique agreed with in vivo results of the low toxicity observed.
32272702	1	16	theme	new	125:127	arg1	materials					143:151	new biocompatible materials	125:151	new biocompatible materials for application in the replacement of deteriorated tissues (due to accidents and diseases)	125:242	The development of new biocompatible materials for application in the replacement of deteriorated tissues (due to accidents and diseases) has gained a lot of attention due to the high demand around the world.
32272702	12	17	theme	films	2107:2111	arg1	cross-section					2086:2098	the cross-section	2082:2098	the cross-section of the films	2082:2111	However, given the volatility of the RGEO, it was possible to generate a slightly porous structure, as can be seen in the microstructure analysis of the surface and the cross-section of the films.
32272702	12	17	theme	films	2107:2111	arg1	analysis					2054:2061	the microstructure analysis	2035:2061	the microstructure analysis of the surface	2035:2076	However, given the volatility of the RGEO, it was possible to generate a slightly porous structure, as can be seen in the microstructure analysis of the surface and the cross-section of the films.
32272702	11	18	theme	understandable	1842:1855	arg1	loss					1857:1860	an understandable loss	1839:1860	an understandable loss of tensile strength	1839:1880	However, mechanical properties demonstrated an understandable loss of tensile strength and Young's modulus for F3 and F4.
32272702	5	19	theme	CS-RGEO	866:872	arg1	%					877:877	CS-RGEO 0.5%	866:877	F2: CS-RGEO 0.5%	862:877	An oil-free formulation (F1: CS) and three different formulations containing R. graveolens essential oil were prepared (F2: CS-RGEO 0.5%; F3: CS+RGEO 1.0%; and F4: CS+RGEO 1.5%) to evaluate the effect of the RGEO incorporation in the mechanical and thermal stability of the films.
32272702	5	20	from	effect	936:941	arg1	stability					999:1007	the mechanical and thermal stability	972:1007	the mechanical and thermal stability of the films	972:1020	An oil-free formulation (F1: CS) and three different formulations containing R. graveolens essential oil were prepared (F2: CS-RGEO 0.5%; F3: CS+RGEO 1.0%; and F4: CS+RGEO 1.5%) to evaluate the effect of the RGEO incorporation in the mechanical and thermal stability of the films.
32272702	5	21	theme	thermal	991:997	arg1	stability					999:1007	the mechanical and thermal stability	972:1007	the mechanical and thermal stability of the films	972:1020	An oil-free formulation (F1: CS) and three different formulations containing R. graveolens essential oil were prepared (F2: CS-RGEO 0.5%; F3: CS+RGEO 1.0%; and F4: CS+RGEO 1.5%) to evaluate the effect of the RGEO incorporation in the mechanical and thermal stability of the films.
32272702	8	22	theme	permeability	1367:1378	arg1	tests					1380:1384	mechanical and water permeability tests	1346:1384	mechanical and water permeability tests	1346:1384	Interesting saturation phenomena were observed for mechanical and water permeability tests when RGEO was incorporated at higher than 0.5% (v/v).
32272702	14	23	contain	have	2324:2327	arg1	films					2288:2292	films	2288:2292	films including crude essential oil	2288:2322	All these results demonstrate that films including crude essential oil have great application potential in the biomedical field.
32272702	14	23	contain	have	2324:2327	arg2	potential					2347:2355	great application potential	2329:2355	great application potential	2329:2355	All these results demonstrate that films including crude essential oil have great application potential in the biomedical field.
32272702	0	24	theme	Essential	85:93	arg1	Films					99:103	Essential Oil Films	85:103	Essential Oil Films	85:103	Synthesis, Characterization, and Histological Evaluation of Chitosan-Ruta Graveolens Essential Oil Films.
32272702	9	25	theme	significant	1611:1621	arg1	inflammation					1623:1634	the material, but also more significant inflammation	1583:1634	the material, but also more significant inflammation of the tissue surrounding the materials	1583:1674	The results of subdermal implantation after 30 days in Wistar rats showed that increasing the amount of RGEO resulted in greater resorption of the material, but also more significant inflammation of the tissue surrounding the materials.
32272702	11	26	theme	strength	1873:1880	arg1	loss					1857:1860	an understandable loss	1839:1860	an understandable loss of tensile strength	1839:1880	However, mechanical properties demonstrated an understandable loss of tensile strength and Young's modulus for F3 and F4.
32272702	11	26	theme	strength	1873:1880	arg1	modulus					1894:1900	Young's modulus	1886:1900	Young's modulus for F3 and F4	1886:1914	However, mechanical properties demonstrated an understandable loss of tensile strength and Young's modulus for F3 and F4.
32272702	13	27	theme	toxicity	2234:2241	arg1	results					2215:2221	in vivo results	2207:2221	in vivo results of the low toxicity observed	2207:2250	The cytotoxicity analysis of the CS+RGEO compositions by the hemolysis technique agreed with in vivo results of the low toxicity observed.
32272702	9	28	from	days	1487:1490	arg1	rats					1502:1505	Wistar rats	1495:1505	Wistar rats	1495:1505	The results of subdermal implantation after 30 days in Wistar rats showed that increasing the amount of RGEO resulted in greater resorption of the material, but also more significant inflammation of the tissue surrounding the materials.
32272702	1	29	theme	materials	143:151	arg1	development					110:120	The development	106:120	The development of new biocompatible materials for application in the replacement of deteriorated tissues (due to accidents and diseases)	106:242	The development of new biocompatible materials for application in the replacement of deteriorated tissues (due to accidents and diseases) has gained a lot of attention due to the high demand around the world.
32272702	2	30	theme	easy	392:395	arg1	resorption					397:406	easy resorption	392:406	easy resorption	392:406	Tissue engineering offers multiple options from biocompatible materials with easy resorption.
32272702	5	31	theme	mechanical	976:985	arg1	stability					999:1007	the mechanical and thermal stability	972:1007	the mechanical and thermal stability of the films	972:1020	An oil-free formulation (F1: CS) and three different formulations containing R. graveolens essential oil were prepared (F2: CS-RGEO 0.5%; F3: CS+RGEO 1.0%; and F4: CS+RGEO 1.5%) to evaluate the effect of the RGEO incorporation in the mechanical and thermal stability of the films.
32272702	9	32	theme	tissue	1643:1648	arg1	inflammation					1623:1634	the material, but also more significant inflammation	1583:1634	the material, but also more significant inflammation of the tissue surrounding the materials	1583:1674	The results of subdermal implantation after 30 days in Wistar rats showed that increasing the amount of RGEO resulted in greater resorption of the material, but also more significant inflammation of the tissue surrounding the materials.
32272702	12	33	theme	surface	2070:2076	arg1	cross-section					2086:2098	the cross-section	2082:2098	the cross-section of the films	2082:2111	However, given the volatility of the RGEO, it was possible to generate a slightly porous structure, as can be seen in the microstructure analysis of the surface and the cross-section of the films.
32272702	12	33	theme	surface	2070:2076	arg1	analysis					2054:2061	the microstructure analysis	2035:2061	the microstructure analysis of the surface	2035:2076	However, given the volatility of the RGEO, it was possible to generate a slightly porous structure, as can be seen in the microstructure analysis of the surface and the cross-section of the films.
32272702	1	34	theme	attention	264:272	arg1	attention					264:272	attention	264:272	attention due to the high demand around the world	264:312	The development of new biocompatible materials for application in the replacement of deteriorated tissues (due to accidents and diseases) has gained a lot of attention due to the high demand around the world.
32272702	1	34	theme	attention	264:272	arg1	lot					257:259	a lot	255:259	a lot of attention due to the high demand around the world	255:312	The development of new biocompatible materials for application in the replacement of deteriorated tissues (due to accidents and diseases) has gained a lot of attention due to the high demand around the world.
32272702	12	35	theme	porous	1999:2004	arg1	structure					2006:2014	a slightly porous structure	1988:2014	a slightly porous structure	1988:2014	However, given the volatility of the RGEO, it was possible to generate a slightly porous structure, as can be seen in the microstructure analysis of the surface and the cross-section of the films.
32272702	1	36	theme	high	285:288	arg1	demand					290:295	the high demand	281:295	the high demand around the world	281:312	The development of new biocompatible materials for application in the replacement of deteriorated tissues (due to accidents and diseases) has gained a lot of attention due to the high demand around the world.
32272702	5	37	theme	1.5	914:916	arg1	%					917:917	%	917:917	%	917:917	An oil-free formulation (F1: CS) and three different formulations containing R. graveolens essential oil were prepared (F2: CS-RGEO 0.5%; F3: CS+RGEO 1.0%; and F4: CS+RGEO 1.5%) to evaluate the effect of the RGEO incorporation in the mechanical and thermal stability of the films.
32272702	2	38	from	materials	377:385	arg1	options					350:356	multiple options	341:356	multiple options from biocompatible materials with easy resorption	341:406	Tissue engineering offers multiple options from biocompatible materials with easy resorption.
32272702	7	39	theme	calorimetry	1159:1169	arg1	analysis					1177:1184	X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analysis	1109:1184	X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analysis	1109:1184	In contrast, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analysis showed that the crystalline structure and percentage of CS were slightly affected by the RGEO incorporation.
32272702	5	40	theme	films	1016:1020	arg1	stability					999:1007	the mechanical and thermal stability	972:1007	the mechanical and thermal stability of the films	972:1020	An oil-free formulation (F1: CS) and three different formulations containing R. graveolens essential oil were prepared (F2: CS-RGEO 0.5%; F3: CS+RGEO 1.0%; and F4: CS+RGEO 1.5%) to evaluate the effect of the RGEO incorporation in the mechanical and thermal stability of the films.
32272702	0	41	theme	Chitosan-Ruta	60:72	arg1	Characterization					11:26	Characterization	11:26	Characterization	11:26	Synthesis, Characterization, and Histological Evaluation of Chitosan-Ruta Graveolens Essential Oil Films.
32272702	0	41	theme	Chitosan-Ruta	60:72	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis, Characterization, and Histological Evaluation of Chitosan-Ruta Graveolens Essential Oil Films.
32272702	0	41	theme	Chitosan-Ruta	60:72	arg1	Evaluation					46:55	Histological Evaluation	33:55	Histological Evaluation	33:55	Synthesis, Characterization, and Histological Evaluation of Chitosan-Ruta Graveolens Essential Oil Films.
32272702	3	42	theme	second	464:469	arg1	polysaccharide					485:498	the second most abundant polysaccharide	460:498	the second most abundant polysaccharide in nature	460:508	Chitosan (CS) is a biopolymer derived from chitin, the second most abundant polysaccharide in nature, which has been highly used for cell regeneration applications.
32272702	3	42	theme	second	464:469	arg1	chitin					452:457	chitin	452:457	chitin	452:457	Chitosan (CS) is a biopolymer derived from chitin, the second most abundant polysaccharide in nature, which has been highly used for cell regeneration applications.
32272702	14	43	theme	great	2329:2333	arg1	potential					2347:2355	great application potential	2329:2355	great application potential	2329:2355	All these results demonstrate that films including crude essential oil have great application potential in the biomedical field.
32272702	4	44	dep	films	591:595	arg1	oil					627:629	essential oil	617:629	essential oil (RGEO)	617:636	In this work, CS films and Ruta graveolens essential oil (RGEO) were incorporated to obtain porous and resorbable materials, which did not generate allergic reactions.
32272702	4	44	dep	films	591:595	arg1	RGEO					632:635	RGEO	632:635	RGEO	632:635	In this work, CS films and Ruta graveolens essential oil (RGEO) were incorporated to obtain porous and resorbable materials, which did not generate allergic reactions.
32272702	7	45	theme	differential	1137:1148	arg1	DSC					1172:1174	DSC	1172:1174	DSC	1172:1174	In contrast, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analysis showed that the crystalline structure and percentage of CS were slightly affected by the RGEO incorporation.
32272702	7	45	theme	differential	1137:1148	arg1	calorimetry					1159:1169	differential scanning calorimetry	1137:1169	differential scanning calorimetry (DSC)	1137:1175	In contrast, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analysis showed that the crystalline structure and percentage of CS were slightly affected by the RGEO incorporation.
32272702	8	46	theme	Interesting	1295:1305	arg1	phenomena					1318:1326	Interesting saturation phenomena	1295:1326	Interesting saturation phenomena	1295:1326	Interesting saturation phenomena were observed for mechanical and water permeability tests when RGEO was incorporated at higher than 0.5% (v/v).
32272702	12	47	theme	RGEO	1954:1957	arg1	volatility					1936:1945	the volatility	1932:1945	the volatility of the RGEO	1932:1957	However, given the volatility of the RGEO, it was possible to generate a slightly porous structure, as can be seen in the microstructure analysis of the surface and the cross-section of the films.
32272702	13	48	theme	CS+RGEO	2147:2153	arg1	compositions					2155:2166	the CS+RGEO compositions	2143:2166	the CS+RGEO compositions	2143:2166	The cytotoxicity analysis of the CS+RGEO compositions by the hemolysis technique agreed with in vivo results of the low toxicity observed.
32272702	2	49	with	materials	377:385	arg1	resorption					397:406	easy resorption	392:406	easy resorption	392:406	Tissue engineering offers multiple options from biocompatible materials with easy resorption.
32272702	13	50	theme	hemolysis	2175:2183	arg1	technique					2185:2193	the hemolysis technique	2171:2193	the hemolysis technique	2171:2193	The cytotoxicity analysis of the CS+RGEO compositions by the hemolysis technique agreed with in vivo results of the low toxicity observed.
32272702	2	51	theme	multiple	341:348	arg1	options					350:356	multiple options	341:356	multiple options from biocompatible materials with easy resorption	341:406	Tissue engineering offers multiple options from biocompatible materials with easy resorption.
32272702	7	52	theme	X-ray	1109:1113	arg1	diffraction					1115:1125	X-ray diffraction	1109:1125	X-ray diffraction (XRD)	1109:1131	In contrast, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analysis showed that the crystalline structure and percentage of CS were slightly affected by the RGEO incorporation.
32272702	7	52	theme	X-ray	1109:1113	arg1	XRD					1128:1130	XRD	1128:1130	XRD	1128:1130	In contrast, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analysis showed that the crystalline structure and percentage of CS were slightly affected by the RGEO incorporation.
32272702	10	53	theme	RGEO	1733:1736	arg1	incorporation					1738:1750	the RGEO incorporation	1729:1750	the RGEO incorporation	1729:1750	On the other hand, the thermal analysis showed that the RGEO incorporation almost did not affect thermal degradation.
32272702	3	54	from	polysaccharide	485:498	arg1	nature					503:508	nature	503:508	nature	503:508	Chitosan (CS) is a biopolymer derived from chitin, the second most abundant polysaccharide in nature, which has been highly used for cell regeneration applications.
32272702	11	55	theme	tensile	1865:1871	arg1	strength					1873:1880	tensile strength	1865:1880	tensile strength	1865:1880	However, mechanical properties demonstrated an understandable loss of tensile strength and Young's modulus for F3 and F4.
32272702	5	56	dep	F1	767:768	arg1	CS					771:772	CS	771:772	CS	771:772	An oil-free formulation (F1: CS) and three different formulations containing R. graveolens essential oil were prepared (F2: CS-RGEO 0.5%; F3: CS+RGEO 1.0%; and F4: CS+RGEO 1.5%) to evaluate the effect of the RGEO incorporation in the mechanical and thermal stability of the films.
32272702	3	57	attach	derived	439:445	arg1	polysaccharide					485:498	the second most abundant polysaccharide	460:498	the second most abundant polysaccharide in nature	460:508	Chitosan (CS) is a biopolymer derived from chitin, the second most abundant polysaccharide in nature, which has been highly used for cell regeneration applications.
32272702	3	57	attach	derived	439:445	arg2	Chitosan					409:416	Chitosan	409:416	Chitosan (CS)	409:421	Chitosan (CS) is a biopolymer derived from chitin, the second most abundant polysaccharide in nature, which has been highly used for cell regeneration applications.
32272702	3	57	attach	derived	439:445	arg2	biopolymer					428:437	a biopolymer	426:437	a biopolymer	426:437	Chitosan (CS) is a biopolymer derived from chitin, the second most abundant polysaccharide in nature, which has been highly used for cell regeneration applications.
32272702	3	57	attach	derived	439:445	arg1	chitin					452:457	chitin	452:457	chitin	452:457	Chitosan (CS) is a biopolymer derived from chitin, the second most abundant polysaccharide in nature, which has been highly used for cell regeneration applications.
32272702	10	58	theme	thermal	1774:1780	arg1	degradation					1782:1792	thermal degradation	1774:1792	thermal degradation	1774:1792	On the other hand, the thermal analysis showed that the RGEO incorporation almost did not affect thermal degradation.
32272702	1	59	theme	deteriorated	191:202	arg1	tissues					204:210	deteriorated tissues	191:210	deteriorated tissues (due to accidents and diseases)	191:242	The development of new biocompatible materials for application in the replacement of deteriorated tissues (due to accidents and diseases) has gained a lot of attention due to the high demand around the world.
32272702	4	60	theme	essential	617:625	arg1	oil					627:629	essential oil	617:629	essential oil (RGEO)	617:636	In this work, CS films and Ruta graveolens essential oil (RGEO) were incorporated to obtain porous and resorbable materials, which did not generate allergic reactions.
32272702	4	60	theme	essential	617:625	arg1	RGEO					632:635	RGEO	632:635	RGEO	632:635	In this work, CS films and Ruta graveolens essential oil (RGEO) were incorporated to obtain porous and resorbable materials, which did not generate allergic reactions.
32272702	5	61	theme	R.	819:820	arg1	oil					843:845	R. graveolens essential oil	819:845	R. graveolens essential oil	819:845	An oil-free formulation (F1: CS) and three different formulations containing R. graveolens essential oil were prepared (F2: CS-RGEO 0.5%; F3: CS+RGEO 1.0%; and F4: CS+RGEO 1.5%) to evaluate the effect of the RGEO incorporation in the mechanical and thermal stability of the films.
32272702	6	62	theme	Infrared	1023:1030	arg1	FTIR					1046:1049	FTIR	1046:1049	FTIR	1046:1049	Infrared spectroscopy (FTIR) analyses demonstrated the presence of RGEO.
32272702	6	62	theme	Infrared	1023:1030	arg1	spectroscopy					1032:1043	Infrared spectroscopy	1023:1043	Infrared spectroscopy (FTIR) analyses	1023:1059	Infrared spectroscopy (FTIR) analyses demonstrated the presence of RGEO.
32272702	9	63	theme	implantation	1465:1476	arg1	results					1444:1450	The results	1440:1450	The results of subdermal implantation after 30 days in Wistar rats	1440:1505	The results of subdermal implantation after 30 days in Wistar rats showed that increasing the amount of RGEO resulted in greater resorption of the material, but also more significant inflammation of the tissue surrounding the materials.
32272702	5	64	theme	graveolens	822:831	arg1	oil					843:845	R. graveolens essential oil	819:845	R. graveolens essential oil	819:845	An oil-free formulation (F1: CS) and three different formulations containing R. graveolens essential oil were prepared (F2: CS-RGEO 0.5%; F3: CS+RGEO 1.0%; and F4: CS+RGEO 1.5%) to evaluate the effect of the RGEO incorporation in the mechanical and thermal stability of the films.
32272702	7	65	dep	structure	1214:1222	arg1	the					1198:1200	the	1198:1200	the	1198:1200	In contrast, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analysis showed that the crystalline structure and percentage of CS were slightly affected by the RGEO incorporation.
32272702	9	66	theme	greater	1561:1567	arg1	resorption					1569:1578	greater resorption	1561:1578	greater resorption of the material, but also more significant inflammation of the tissue surrounding the materials	1561:1674	The results of subdermal implantation after 30 days in Wistar rats showed that increasing the amount of RGEO resulted in greater resorption of the material, but also more significant inflammation of the tissue surrounding the materials.
32272702	4	67	theme	Ruta	601:604	arg1	graveolens					606:615	Ruta graveolens	601:615	Ruta graveolens	601:615	In this work, CS films and Ruta graveolens essential oil (RGEO) were incorporated to obtain porous and resorbable materials, which did not generate allergic reactions.
32272702	5	68	theme	incorporation	955:967	arg1	effect					936:941	the effect	932:941	the effect of the RGEO incorporation in the mechanical and thermal stability of the films	932:1020	An oil-free formulation (F1: CS) and three different formulations containing R. graveolens essential oil were prepared (F2: CS-RGEO 0.5%; F3: CS+RGEO 1.0%; and F4: CS+RGEO 1.5%) to evaluate the effect of the RGEO incorporation in the mechanical and thermal stability of the films.
32272702	3	69	theme	regeneration	547:558	arg1	applications					560:571	cell regeneration applications	542:571	cell regeneration applications	542:571	Chitosan (CS) is a biopolymer derived from chitin, the second most abundant polysaccharide in nature, which has been highly used for cell regeneration applications.
32272702	8	70	theme	mechanical	1346:1355	arg1	tests					1380:1384	mechanical and water permeability tests	1346:1384	mechanical and water permeability tests	1346:1384	Interesting saturation phenomena were observed for mechanical and water permeability tests when RGEO was incorporated at higher than 0.5% (v/v).
32272702	6	71	theme	RGEO	1090:1093	arg1	presence					1078:1085	the presence	1074:1085	the presence of RGEO	1074:1093	Infrared spectroscopy (FTIR) analyses demonstrated the presence of RGEO.
32272702	12	72	theme	microstructure	2039:2052	arg1	analysis					2054:2061	the microstructure analysis	2035:2061	the microstructure analysis of the surface	2035:2076	However, given the volatility of the RGEO, it was possible to generate a slightly porous structure, as can be seen in the microstructure analysis of the surface and the cross-section of the films.
32272702	10	73	theme	other	1684:1688	arg1	hand					1690:1693	the other hand	1680:1693	the other hand	1680:1693	On the other hand, the thermal analysis showed that the RGEO incorporation almost did not affect thermal degradation.
32272702	9	74	theme	material	1587:1594	arg1	inflammation					1623:1634	the material, but also more significant inflammation	1583:1634	the material, but also more significant inflammation of the tissue surrounding the materials	1583:1674	The results of subdermal implantation after 30 days in Wistar rats showed that increasing the amount of RGEO resulted in greater resorption of the material, but also more significant inflammation of the tissue surrounding the materials.
32272702	8	75	theme	water	1361:1365	arg1	permeability					1367:1378	water permeability	1361:1378	water permeability	1361:1378	Interesting saturation phenomena were observed for mechanical and water permeability tests when RGEO was incorporated at higher than 0.5% (v/v).
32272702	13	76	theme	in	2207:2208	arg1	results					2215:2221	in vivo results	2207:2221	in vivo results of the low toxicity observed	2207:2250	The cytotoxicity analysis of the CS+RGEO compositions by the hemolysis technique agreed with in vivo results of the low toxicity observed.
32272702	5	77	dep	CS+RGEO	884:890	arg1	%					895:895	1.0%	892:895	CS+RGEO 1.0%	884:895	An oil-free formulation (F1: CS) and three different formulations containing R. graveolens essential oil were prepared (F2: CS-RGEO 0.5%; F3: CS+RGEO 1.0%; and F4: CS+RGEO 1.5%) to evaluate the effect of the RGEO incorporation in the mechanical and thermal stability of the films.
32272702	5	78	theme	essential	833:841	arg1	oil					843:845	R. graveolens essential oil	819:845	R. graveolens essential oil	819:845	An oil-free formulation (F1: CS) and three different formulations containing R. graveolens essential oil were prepared (F2: CS-RGEO 0.5%; F3: CS+RGEO 1.0%; and F4: CS+RGEO 1.5%) to evaluate the effect of the RGEO incorporation in the mechanical and thermal stability of the films.
32272702	7	79	theme	crystalline	1202:1212	arg1	structure					1214:1222	crystalline structure	1202:1222	crystalline structure	1202:1222	In contrast, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analysis showed that the crystalline structure and percentage of CS were slightly affected by the RGEO incorporation.
32272702	0	80	theme	Oil	95:97	arg1	Films					99:103	Essential Oil Films	85:103	Essential Oil Films	85:103	Synthesis, Characterization, and Histological Evaluation of Chitosan-Ruta Graveolens Essential Oil Films.
32272702	13	81	theme	low	2230:2232	arg1	toxicity					2234:2241	the low toxicity	2226:2241	the low toxicity observed	2226:2250	The cytotoxicity analysis of the CS+RGEO compositions by the hemolysis technique agreed with in vivo results of the low toxicity observed.
32272702	9	82	theme	inflammation	1623:1634	arg1	resorption					1569:1578	greater resorption	1561:1578	greater resorption of the material, but also more significant inflammation of the tissue surrounding the materials	1561:1674	The results of subdermal implantation after 30 days in Wistar rats showed that increasing the amount of RGEO resulted in greater resorption of the material, but also more significant inflammation of the tissue surrounding the materials.
32272702	5	83	contain	containing	808:817	arg2	oil					843:845	R. graveolens essential oil	819:845	R. graveolens essential oil	819:845	An oil-free formulation (F1: CS) and three different formulations containing R. graveolens essential oil were prepared (F2: CS-RGEO 0.5%; F3: CS+RGEO 1.0%; and F4: CS+RGEO 1.5%) to evaluate the effect of the RGEO incorporation in the mechanical and thermal stability of the films.
32272702	5	83	contain	containing	808:817	arg1	formulations					795:806	three different formulations	779:806	three different formulations containing R. graveolens essential oil	779:845	An oil-free formulation (F1: CS) and three different formulations containing R. graveolens essential oil were prepared (F2: CS-RGEO 0.5%; F3: CS+RGEO 1.0%; and F4: CS+RGEO 1.5%) to evaluate the effect of the RGEO incorporation in the mechanical and thermal stability of the films.
32272702	1	84	from	application	157:167	arg1	replacement					176:186	the replacement	172:186	the replacement of deteriorated tissues (due to accidents and diseases)	172:242	The development of new biocompatible materials for application in the replacement of deteriorated tissues (due to accidents and diseases) has gained a lot of attention due to the high demand around the world.
32272702	14	85	theme	biomedical	2364:2373	arg1	field					2375:2379	the biomedical field	2360:2379	the biomedical field	2360:2379	All these results demonstrate that films including crude essential oil have great application potential in the biomedical field.
32272702	5	86	dep	F2	862:863	arg1	%					877:877	CS-RGEO 0.5%	866:877	F2: CS-RGEO 0.5%	862:877	An oil-free formulation (F1: CS) and three different formulations containing R. graveolens essential oil were prepared (F2: CS-RGEO 0.5%; F3: CS+RGEO 1.0%; and F4: CS+RGEO 1.5%) to evaluate the effect of the RGEO incorporation in the mechanical and thermal stability of the films.
32272702	5	86	dep	F2	862:863	arg1	CS+RGEO					906:912	CS+RGEO	906:912	CS+RGEO	906:912	An oil-free formulation (F1: CS) and three different formulations containing R. graveolens essential oil were prepared (F2: CS-RGEO 0.5%; F3: CS+RGEO 1.0%; and F4: CS+RGEO 1.5%) to evaluate the effect of the RGEO incorporation in the mechanical and thermal stability of the films.
32272702	14	87	theme	essential	2310:2318	arg1	oil					2320:2322	crude essential oil	2304:2322	crude essential oil	2304:2322	All these results demonstrate that films including crude essential oil have great application potential in the biomedical field.
32272702	5	88	dep	prepared	852:859	arg1	F4					902:903	F4	902:903	F4	902:903	An oil-free formulation (F1: CS) and three different formulations containing R. graveolens essential oil were prepared (F2: CS-RGEO 0.5%; F3: CS+RGEO 1.0%; and F4: CS+RGEO 1.5%) to evaluate the effect of the RGEO incorporation in the mechanical and thermal stability of the films.
32272702	5	88	dep	prepared	852:859	arg1	F2					862:863	F2	862:863	F2	862:863	An oil-free formulation (F1: CS) and three different formulations containing R. graveolens essential oil were prepared (F2: CS-RGEO 0.5%; F3: CS+RGEO 1.0%; and F4: CS+RGEO 1.5%) to evaluate the effect of the RGEO incorporation in the mechanical and thermal stability of the films.
32272702	5	88	dep	prepared	852:859	arg1	F3					880:881	F3	880:881	F3	880:881	An oil-free formulation (F1: CS) and three different formulations containing R. graveolens essential oil were prepared (F2: CS-RGEO 0.5%; F3: CS+RGEO 1.0%; and F4: CS+RGEO 1.5%) to evaluate the effect of the RGEO incorporation in the mechanical and thermal stability of the films.
32272702	5	88	dep	prepared	852:859	arg1	CS+RGEO					884:890	CS+RGEO	884:890	CS+RGEO	884:890	An oil-free formulation (F1: CS) and three different formulations containing R. graveolens essential oil were prepared (F2: CS-RGEO 0.5%; F3: CS+RGEO 1.0%; and F4: CS+RGEO 1.5%) to evaluate the effect of the RGEO incorporation in the mechanical and thermal stability of the films.
32272702	2	89	theme	biocompatible	363:375	arg1	materials					377:385	biocompatible materials	363:385	biocompatible materials with easy resorption	363:406	Tissue engineering offers multiple options from biocompatible materials with easy resorption.
32272702	1	90	theme	due	274:276	arg1	attention					264:272	attention	264:272	attention due to the high demand around the world	264:312	The development of new biocompatible materials for application in the replacement of deteriorated tissues (due to accidents and diseases) has gained a lot of attention due to the high demand around the world.
32272702	4	91	dep	porous	666:671	arg1	materials					688:696	materials	688:696	materials	688:696	In this work, CS films and Ruta graveolens essential oil (RGEO) were incorporated to obtain porous and resorbable materials, which did not generate allergic reactions.
32272702	10	92	theme	thermal	1700:1706	arg1	analysis					1708:1715	the thermal analysis	1696:1715	the thermal analysis	1696:1715	On the other hand, the thermal analysis showed that the RGEO incorporation almost did not affect thermal degradation.
32272702	0	93	theme	Histological	33:44	arg1	Evaluation					46:55	Histological Evaluation	33:55	Histological Evaluation	33:55	Synthesis, Characterization, and Histological Evaluation of Chitosan-Ruta Graveolens Essential Oil Films.
32272702	7	94	theme	scanning	1150:1157	arg1	DSC					1172:1174	DSC	1172:1174	DSC	1172:1174	In contrast, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analysis showed that the crystalline structure and percentage of CS were slightly affected by the RGEO incorporation.
32272702	7	94	theme	scanning	1150:1157	arg1	calorimetry					1159:1169	differential scanning calorimetry	1137:1169	differential scanning calorimetry (DSC)	1137:1175	In contrast, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analysis showed that the crystalline structure and percentage of CS were slightly affected by the RGEO incorporation.
32272702	1	95	dep	tissues	204:210	arg1	due					213:215	due	213:215	due	213:215	The development of new biocompatible materials for application in the replacement of deteriorated tissues (due to accidents and diseases) has gained a lot of attention due to the high demand around the world.
32272702	5	96	theme	oil-free	745:752	arg1	F1					767:768	F1	767:768	F1	767:768	An oil-free formulation (F1: CS) and three different formulations containing R. graveolens essential oil were prepared (F2: CS-RGEO 0.5%; F3: CS+RGEO 1.0%; and F4: CS+RGEO 1.5%) to evaluate the effect of the RGEO incorporation in the mechanical and thermal stability of the films.
32272702	5	96	theme	oil-free	745:752	arg1	formulation					754:764	An oil-free formulation	742:764	An oil-free formulation (F1: CS)	742:773	An oil-free formulation (F1: CS) and three different formulations containing R. graveolens essential oil were prepared (F2: CS-RGEO 0.5%; F3: CS+RGEO 1.0%; and F4: CS+RGEO 1.5%) to evaluate the effect of the RGEO incorporation in the mechanical and thermal stability of the films.
32272702	14	97	theme	application	2335:2345	arg1	potential					2347:2355	great application potential	2329:2355	great application potential	2329:2355	All these results demonstrate that films including crude essential oil have great application potential in the biomedical field.
32272702	9	98	theme	subdermal	1455:1463	arg1	implantation					1465:1476	subdermal implantation	1455:1476	subdermal implantation after 30 days in Wistar rats	1455:1505	The results of subdermal implantation after 30 days in Wistar rats showed that increasing the amount of RGEO resulted in greater resorption of the material, but also more significant inflammation of the tissue surrounding the materials.
32272702	14	99	theme	crude	2304:2308	arg1	oil					2320:2322	crude essential oil	2304:2322	crude essential oil	2304:2322	All these results demonstrate that films including crude essential oil have great application potential in the biomedical field.
32272702	2	100	theme	Tissue	315:320	arg1	engineering					322:332	Tissue engineering	315:332	Tissue engineering	315:332	Tissue engineering offers multiple options from biocompatible materials with easy resorption.
32272702	13	101	theme	compositions	2155:2166	arg1	analysis					2131:2138	The cytotoxicity analysis	2114:2138	The cytotoxicity analysis of the CS+RGEO compositions by the hemolysis technique	2114:2193	The cytotoxicity analysis of the CS+RGEO compositions by the hemolysis technique agreed with in vivo results of the low toxicity observed.
32272702	1	102	dep	new	125:127	arg1	biocompatible					129:141	biocompatible	129:141	biocompatible	129:141	The development of new biocompatible materials for application in the replacement of deteriorated tissues (due to accidents and diseases) has gained a lot of attention due to the high demand around the world.
32272702	13	103	theme	cytotoxicity	2118:2129	arg1	analysis					2131:2138	The cytotoxicity analysis	2114:2138	The cytotoxicity analysis of the CS+RGEO compositions by the hemolysis technique	2114:2193	The cytotoxicity analysis of the CS+RGEO compositions by the hemolysis technique agreed with in vivo results of the low toxicity observed.
32272702	7	104	theme	diffraction	1115:1125	arg1	analysis					1177:1184	X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analysis	1109:1184	X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analysis	1109:1184	In contrast, X-ray diffraction (XRD) and differential scanning calorimetry (DSC) analysis showed that the crystalline structure and percentage of CS were slightly affected by the RGEO incorporation.
32272702	4	105	theme	CS	588:589	arg1	films					591:595	CS films	588:595	CS films	588:595	In this work, CS films and Ruta graveolens essential oil (RGEO) were incorporated to obtain porous and resorbable materials, which did not generate allergic reactions.
32371134	2	0	theme	inflammatory	444:455	arg1	IBD					472:474	IBD	472:474	IBD	472:474	In the present study, effects of TFPS on compositions of gut microbiota of feces from healthy persons and patients with inflammatory bowel disease (IBD) were investigated in vitro.
32371134	2	0	theme	inflammatory	444:455	arg1	disease					463:469	inflammatory bowel disease	444:469	inflammatory bowel disease (IBD)	444:475	In the present study, effects of TFPS on compositions of gut microbiota of feces from healthy persons and patients with inflammatory bowel disease (IBD) were investigated in vitro.
32371134	1	1	theme	distal	305:310	arg1	intestines					312:321	the distal intestines	301:321	the distal intestines	301:321	Polysaccharides from the flowers of tea (Camellia sinensis L.) plant (TFPS) were considered as potential functional components mainly playing the role in the distal intestines.
32371134	1	2	from	role	293:296	arg1	intestines					312:321	the distal intestines	301:321	the distal intestines	301:321	Polysaccharides from the flowers of tea (Camellia sinensis L.) plant (TFPS) were considered as potential functional components mainly playing the role in the distal intestines.
32371134	1	3	from	flowers	172:178	arg1	components					263:272	potential functional components	242:272	potential functional components mainly playing the role in the distal intestines	242:321	Polysaccharides from the flowers of tea (Camellia sinensis L.) plant (TFPS) were considered as potential functional components mainly playing the role in the distal intestines.
32371134	1	3	from	flowers	172:178	arg1	Polysaccharides					147:161	Polysaccharides	147:161	Polysaccharides from the flowers of tea (Camellia sinensis L.) plant (TFPS)	147:221	Polysaccharides from the flowers of tea (Camellia sinensis L.) plant (TFPS) were considered as potential functional components mainly playing the role in the distal intestines.
32371134	0	4	theme	gut	67:69	arg1	microbiota					71:80	gut microbiota	67:80	gut microbiota of healthy persons and patients with inflammatory bowel disease	67:144	Prebiotics effects in vitro of polysaccharides from tea flowers on gut microbiota of healthy persons and patients with inflammatory bowel disease.
32371134	0	5	from	effects	11:17	arg1	flowers					56:62	tea flowers	52:62	tea flowers on gut microbiota of healthy persons and patients with inflammatory bowel disease	52:144	Prebiotics effects in vitro of polysaccharides from tea flowers on gut microbiota of healthy persons and patients with inflammatory bowel disease.
32371134	4	6	theme	anaerobic	638:646	arg1	conditions					648:657	simulated anaerobic conditions	628:657	simulated anaerobic conditions	628:657	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	7	dep	Lactobacillus	1105:1117	arg1	Collinsella					1092:1102	Collinsella	1092:1102	Collinsella	1092:1102	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	8	theme	Lactobacillus	822:834	arg1	abundances					759:768	the relative abundances	746:768	the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa	746:899	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	0	9	theme	healthy	85:91	arg1	persons					93:99	healthy persons	85:99	healthy persons	85:99	Prebiotics effects in vitro of polysaccharides from tea flowers on gut microbiota of healthy persons and patients with inflammatory bowel disease.
32371134	4	10	theme	Bifidobacterium	840:854	arg1	abundances					759:768	the relative abundances	746:768	the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa	746:899	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	11	theme	Dialister	785:793	arg1	abundances					759:768	the relative abundances	746:768	the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa	746:899	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	12	theme	intestinal	716:725	arg1	XVIII					985:989	Clostridium XVIII	973:989	Clostridium XVIII	973:989	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	12	theme	intestinal	716:725	arg1	Bifidobacterium					1123:1137	Bifidobacterium	1123:1137	Bifidobacterium	1123:1137	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	12	theme	intestinal	716:725	arg1	Akkermansia					931:941	Akkermansia	931:941	Akkermansia	931:941	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	12	theme	intestinal	716:725	arg1	Alistipes					902:910	Alistipes	902:910	Alistipes	902:910	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	12	theme	intestinal	716:725	arg1	microbes					727:734	intestinal microbes	716:734	intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces)	716:1302	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	12	theme	intestinal	716:725	arg1	Ruminococcus					992:1003	Ruminococcus	992:1003	Ruminococcus	992:1003	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	12	theme	intestinal	716:725	arg1	Butyricicoccus					1009:1022	Butyricicoccus	1009:1022	Butyricicoccus	1009:1022	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	12	theme	intestinal	716:725	arg1	XlVb					925:928	Clostridium XlVb	913:928	Clostridium XlVb	913:928	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	12	theme	intestinal	716:725	arg1	acids					1345:1349	short-chain fatty acids	1327:1349	short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces)	1327:1465	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	12	theme	intestinal	716:725	arg1	Butyricimonas					958:970	Butyricimonas	958:970	Butyricimonas	958:970	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	12	theme	intestinal	716:725	arg1	Lactobacillus					1105:1117	Lactobacillus	1105:1117	Lactobacillus	1105:1117	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	13	theme	normal	1032:1037	arg1	feces					1039:1043	the normal feces	1028:1043	the normal feces	1028:1043	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	5	14	theme	intestinal	1556:1565	arg1	environments					1567:1578	the normal and IBD intestinal environments	1537:1578	environments	1567:1578	The results illustrated that TFPS had probiotics promoting effect on the normal and IBD intestinal environments, however, might have a pro-inflammatory effect on IBD.
32371134	2	15	from	persons	418:424	arg1	feces					399:403	feces	399:403	feces from healthy persons and patients with inflammatory bowel disease (IBD)	399:475	In the present study, effects of TFPS on compositions of gut microbiota of feces from healthy persons and patients with inflammatory bowel disease (IBD) were investigated in vitro.
32371134	2	15	from	persons	418:424	arg1	microbiota					385:394	gut microbiota	381:394	gut microbiota of feces from healthy persons and patients with inflammatory bowel disease (IBD)	381:475	In the present study, effects of TFPS on compositions of gut microbiota of feces from healthy persons and patients with inflammatory bowel disease (IBD) were investigated in vitro.
32371134	4	16	theme	Clostridium	973:983	arg1	XVIII					985:989	Clostridium XVIII	973:989	Clostridium XVIII	973:989	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	16	theme	Clostridium	973:983	arg1	microbes					727:734	intestinal microbes	716:734	intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces)	716:1302	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	17	theme	short-chain	1327:1337	arg1	microbes					727:734	intestinal microbes	716:734	intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces)	716:1302	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	17	theme	short-chain	1327:1337	arg1	acids					1345:1349	short-chain fatty acids	1327:1349	short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces)	1327:1465	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	5	18	from	effect	1620:1625	arg1	IBD					1630:1632	IBD	1630:1632	IBD	1630:1632	The results illustrated that TFPS had probiotics promoting effect on the normal and IBD intestinal environments, however, might have a pro-inflammatory effect on IBD.
32371134	4	19	dep	Alistipes	902:910	arg1	Ruminococcus					944:955	the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus	746:1022	Ruminococcus	944:955	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	19	dep	Alistipes	902:910	arg1	Escherichia/Shigella					1056:1075	increased Escherichia/Shigella	1046:1075	the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella	746:1075	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	2	20	theme	TFPS	357:360	arg1	effects					346:352	effects	346:352	effects of TFPS on compositions of gut microbiota of feces from healthy persons and patients with inflammatory bowel disease (IBD)	346:475	In the present study, effects of TFPS on compositions of gut microbiota of feces from healthy persons and patients with inflammatory bowel disease (IBD) were investigated in vitro.
32371134	0	21	theme	persons	93:99	arg1	microbiota					71:80	gut microbiota	67:80	gut microbiota of healthy persons and patients with inflammatory bowel disease	67:144	Prebiotics effects in vitro of polysaccharides from tea flowers on gut microbiota of healthy persons and patients with inflammatory bowel disease.
32371134	2	22	from	patients	430:437	arg1	feces					399:403	feces	399:403	feces from healthy persons and patients with inflammatory bowel disease (IBD)	399:475	In the present study, effects of TFPS on compositions of gut microbiota of feces from healthy persons and patients with inflammatory bowel disease (IBD) were investigated in vitro.
32371134	2	22	from	patients	430:437	arg1	microbiota					385:394	gut microbiota	381:394	gut microbiota of feces from healthy persons and patients with inflammatory bowel disease (IBD)	381:475	In the present study, effects of TFPS on compositions of gut microbiota of feces from healthy persons and patients with inflammatory bowel disease (IBD) were investigated in vitro.
32371134	4	23	from	12.88 ± 1.59 mM	1372:1386	arg1	microbes					727:734	intestinal microbes	716:734	intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces)	716:1302	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	23	from	12.88 ± 1.59 mM	1372:1386	arg1	acids					1345:1349	short-chain fatty acids	1327:1349	short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces)	1327:1465	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	24	theme	microbes	727:734	arg1	composition					701:711	the composition	697:711	the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces)	697:1465	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	5	25	theme	pro-inflammatory	1603:1618	arg1	effect					1620:1625	a pro-inflammatory effect	1601:1625	a pro-inflammatory effect on IBD	1601:1632	The results illustrated that TFPS had probiotics promoting effect on the normal and IBD intestinal environments, however, might have a pro-inflammatory effect on IBD.
32371134	5	26	contain	have	1596:1599	arg2	effect					1620:1625	a pro-inflammatory effect	1601:1625	a pro-inflammatory effect on IBD	1601:1632	The results illustrated that TFPS had probiotics promoting effect on the normal and IBD intestinal environments, however, might have a pro-inflammatory effect on IBD.
32371134	5	26	contain	have	1596:1599	arg1	TFPS					1497:1500	TFPS	1497:1500	TFPS had probiotics promoting effect on the normal and IBD intestinal environments	1497:1578	The results illustrated that TFPS had probiotics promoting effect on the normal and IBD intestinal environments, however, might have a pro-inflammatory effect on IBD.
32371134	4	27	theme	metabolites	1314:1324	arg1	composition					701:711	the composition	697:711	the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces)	697:1465	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	0	28	with	persons	93:99	arg1	disease					138:144	inflammatory bowel disease	119:144	inflammatory bowel disease	119:144	Prebiotics effects in vitro of polysaccharides from tea flowers on gut microbiota of healthy persons and patients with inflammatory bowel disease.
32371134	5	29	theme	probiotics	1506:1515	arg1	effect					1527:1532	probiotics promoting effect	1506:1532	probiotics promoting effect on the normal and IBD intestinal environments	1506:1578	The results illustrated that TFPS had probiotics promoting effect on the normal and IBD intestinal environments, however, might have a pro-inflammatory effect on IBD.
32371134	2	30	theme	feces	399:403	arg1	microbiota					385:394	gut microbiota	381:394	gut microbiota of feces from healthy persons and patients with inflammatory bowel disease (IBD)	381:475	In the present study, effects of TFPS on compositions of gut microbiota of feces from healthy persons and patients with inflammatory bowel disease (IBD) were investigated in vitro.
32371134	1	31	theme	potential	242:250	arg1	components					263:272	potential functional components	242:272	potential functional components mainly playing the role in the distal intestines	242:321	Polysaccharides from the flowers of tea (Camellia sinensis L.) plant (TFPS) were considered as potential functional components mainly playing the role in the distal intestines.
32371134	1	31	theme	potential	242:250	arg1	Polysaccharides					147:161	Polysaccharides	147:161	Polysaccharides from the flowers of tea (Camellia sinensis L.) plant (TFPS)	147:221	Polysaccharides from the flowers of tea (Camellia sinensis L.) plant (TFPS) were considered as potential functional components mainly playing the role in the distal intestines.
32371134	5	32	theme	IBD	1552:1554	arg1	environments					1567:1578	the normal and IBD intestinal environments	1537:1578	environments	1567:1578	The results illustrated that TFPS had probiotics promoting effect on the normal and IBD intestinal environments, however, might have a pro-inflammatory effect on IBD.
32371134	3	33	theme	normal	530:535	arg1	feces					537:541	normal feces	530:541	normal feces	530:541	Microbial communities in normal feces were more diverse than those in IBD feces.
32371134	1	34	theme	tea	183:185	arg1	TFPS					217:220	tea (Camellia sinensis L.) plant (TFPS)	183:221	tea (Camellia sinensis L.) plant (TFPS)	183:221	Polysaccharides from the flowers of tea (Camellia sinensis L.) plant (TFPS) were considered as potential functional components mainly playing the role in the distal intestines.
32371134	4	35	from	7.61 ± 0.81 mM	1433:1446	arg1	microbes					727:734	intestinal microbes	716:734	intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces)	716:1302	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	35	from	7.61 ± 0.81 mM	1433:1446	arg1	acids					1345:1349	short-chain fatty acids	1327:1349	short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces)	1327:1465	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	1	36	theme	functional	252:261	arg1	components					263:272	potential functional components	242:272	potential functional components mainly playing the role in the distal intestines	242:321	Polysaccharides from the flowers of tea (Camellia sinensis L.) plant (TFPS) were considered as potential functional components mainly playing the role in the distal intestines.
32371134	1	36	theme	functional	252:261	arg1	Polysaccharides					147:161	Polysaccharides	147:161	Polysaccharides from the flowers of tea (Camellia sinensis L.) plant (TFPS)	147:221	Polysaccharides from the flowers of tea (Camellia sinensis L.) plant (TFPS) were considered as potential functional components mainly playing the role in the distal intestines.
32371134	4	37	dep	Akkermansia	1255:1265	arg1	Megasphaera					1215:1225	Megasphaera	1215:1225	Megasphaera	1215:1225	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	3	38	theme	Microbial	505:513	arg1	communities					515:525	Microbial communities	505:525	Microbial communities in normal feces	505:541	Microbial communities in normal feces were more diverse than those in IBD feces.
32371134	2	39	theme	gut	381:383	arg1	microbiota					385:394	gut microbiota	381:394	gut microbiota of feces from healthy persons and patients with inflammatory bowel disease (IBD)	381:475	In the present study, effects of TFPS on compositions of gut microbiota of feces from healthy persons and patients with inflammatory bowel disease (IBD) were investigated in vitro.
32371134	2	40	theme	microbiota	385:394	arg1	compositions					365:376	compositions	365:376	compositions of gut microbiota of feces from healthy persons and patients with inflammatory bowel disease (IBD)	365:475	In the present study, effects of TFPS on compositions of gut microbiota of feces from healthy persons and patients with inflammatory bowel disease (IBD) were investigated in vitro.
32371134	0	41	theme	patients	105:112	arg1	microbiota					71:80	gut microbiota	67:80	gut microbiota of healthy persons and patients with inflammatory bowel disease	67:144	Prebiotics effects in vitro of polysaccharides from tea flowers on gut microbiota of healthy persons and patients with inflammatory bowel disease.
32371134	4	42	theme	IBD	1293:1295	arg1	feces					1297:1301	the IBD feces	1289:1301	the IBD feces	1289:1301	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	0	43	theme	polysaccharides	31:45	arg1	effects					11:17	effects	11:17	effects	11:17	Prebiotics effects in vitro of polysaccharides from tea flowers on gut microbiota of healthy persons and patients with inflammatory bowel disease.
32371134	4	44	theme	normal	1396:1401	arg1	feces					1403:1407	the normal feces	1392:1407	the normal feces	1392:1407	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	0	45	theme	bowel	132:136	arg1	disease					138:144	inflammatory bowel disease	119:144	inflammatory bowel disease	119:144	Prebiotics effects in vitro of polysaccharides from tea flowers on gut microbiota of healthy persons and patients with inflammatory bowel disease.
32371134	4	46	theme	TFPS	608:611	arg1	fermentation					592:603	fermentation	592:603	fermentation of TFPS for 24 h under simulated anaerobic conditions	592:657	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	47	located	observed	685:692	arg2	changes					672:678	significant changes	660:678	significant changes	660:678	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	47	located	observed	685:692	arg1	composition					701:711	the composition	697:711	the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces)	697:1465	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	0	48	theme	inflammatory	119:130	arg1	disease					138:144	inflammatory bowel disease	119:144	inflammatory bowel disease	119:144	Prebiotics effects in vitro of polysaccharides from tea flowers on gut microbiota of healthy persons and patients with inflammatory bowel disease.
32371134	2	49	from	effects	346:352	arg1	compositions					365:376	compositions	365:376	compositions of gut microbiota of feces from healthy persons and patients with inflammatory bowel disease (IBD)	365:475	In the present study, effects of TFPS on compositions of gut microbiota of feces from healthy persons and patients with inflammatory bowel disease (IBD) were investigated in vitro.
32371134	0	50	theme	tea	52:54	arg1	flowers					56:62	tea flowers	52:62	tea flowers on gut microbiota of healthy persons and patients with inflammatory bowel disease	52:144	Prebiotics effects in vitro of polysaccharides from tea flowers on gut microbiota of healthy persons and patients with inflammatory bowel disease.
32371134	4	51	theme	Clostridium	913:923	arg1	XlVb					925:928	Clostridium XlVb	913:928	Clostridium XlVb	913:928	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	51	theme	Clostridium	913:923	arg1	microbes					727:734	intestinal microbes	716:734	intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces)	716:1302	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	52	theme	decreased	862:870	arg1	Prevotella					872:881	decreased Prevotella	862:881	decreased Prevotella	862:881	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	0	53	from	flowers	56:62	arg1	polysaccharides					31:45	polysaccharides	31:45	polysaccharides from tea flowers on gut microbiota of healthy persons and patients with inflammatory bowel disease	31:144	Prebiotics effects in vitro of polysaccharides from tea flowers on gut microbiota of healthy persons and patients with inflammatory bowel disease.
32371134	0	53	from	flowers	56:62	arg1	microbiota					71:80	gut microbiota	67:80	gut microbiota of healthy persons and patients with inflammatory bowel disease	67:144	Prebiotics effects in vitro of polysaccharides from tea flowers on gut microbiota of healthy persons and patients with inflammatory bowel disease.
32371134	0	53	from	flowers	56:62	arg1	effects					11:17	effects	11:17	effects	11:17	Prebiotics effects in vitro of polysaccharides from tea flowers on gut microbiota of healthy persons and patients with inflammatory bowel disease.
32371134	2	54	from	microbiota	385:394	arg1	persons					418:424	healthy persons	410:424	healthy persons	410:424	In the present study, effects of TFPS on compositions of gut microbiota of feces from healthy persons and patients with inflammatory bowel disease (IBD) were investigated in vitro.
32371134	2	54	from	microbiota	385:394	arg1	patients					430:437	patients	430:437	patients with inflammatory bowel disease (IBD)	430:475	In the present study, effects of TFPS on compositions of gut microbiota of feces from healthy persons and patients with inflammatory bowel disease (IBD) were investigated in vitro.
32371134	4	55	theme	significant	660:670	arg1	changes					672:678	significant changes	660:678	significant changes	660:678	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	2	56	theme	healthy	410:416	arg1	persons					418:424	healthy persons	410:424	healthy persons	410:424	In the present study, effects of TFPS on compositions of gut microbiota of feces from healthy persons and patients with inflammatory bowel disease (IBD) were investigated in vitro.
32371134	4	57	dep	12.88 ± 1.59 mM	1372:1386	arg1	to					1369:1370	to	1369:1370	to	1369:1370	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	58	theme	increased	1046:1054	arg1	Escherichia/Shigella					1056:1075	increased Escherichia/Shigella	1046:1075	the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella	746:1075	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	59	theme	relative	750:757	arg1	abundances					759:768	the relative abundances	746:768	the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa	746:899	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	5	60	theme	promoting	1517:1525	arg1	effect					1527:1532	probiotics promoting effect	1506:1532	probiotics promoting effect on the normal and IBD intestinal environments	1506:1578	The results illustrated that TFPS had probiotics promoting effect on the normal and IBD intestinal environments, however, might have a pro-inflammatory effect on IBD.
32371134	5	61	from	effect	1527:1532	arg1	environments					1567:1578	the normal and IBD intestinal environments	1537:1578	environments	1567:1578	The results illustrated that TFPS had probiotics promoting effect on the normal and IBD intestinal environments, however, might have a pro-inflammatory effect on IBD.
32371134	5	61	from	effect	1527:1532	arg1	normal					1541:1546	normal	1541:1546	normal	1541:1546	The results illustrated that TFPS had probiotics promoting effect on the normal and IBD intestinal environments, however, might have a pro-inflammatory effect on IBD.
32371134	3	62	from	communities	515:525	arg1	feces					537:541	normal feces	530:541	normal feces	530:541	Microbial communities in normal feces were more diverse than those in IBD feces.
32371134	3	63	theme	IBD	575:577	arg1	feces					579:583	IBD feces	575:583	IBD feces	575:583	Microbial communities in normal feces were more diverse than those in IBD feces.
32371134	2	64	with	persons	418:424	arg1	IBD					472:474	IBD	472:474	IBD	472:474	In the present study, effects of TFPS on compositions of gut microbiota of feces from healthy persons and patients with inflammatory bowel disease (IBD) were investigated in vitro.
32371134	2	64	with	persons	418:424	arg1	disease					463:469	inflammatory bowel disease	444:469	inflammatory bowel disease (IBD)	444:475	In the present study, effects of TFPS on compositions of gut microbiota of feces from healthy persons and patients with inflammatory bowel disease (IBD) were investigated in vitro.
32371134	4	65	dep	microbes	727:734	arg1	XVIII					985:989	Clostridium XVIII	973:989	Clostridium XVIII	973:989	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	65	dep	microbes	727:734	arg1	Bifidobacterium					1123:1137	Bifidobacterium	1123:1137	Bifidobacterium	1123:1137	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	65	dep	microbes	727:734	arg1	Akkermansia					931:941	Akkermansia	931:941	Akkermansia	931:941	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	65	dep	microbes	727:734	arg1	Alistipes					902:910	Alistipes	902:910	Alistipes	902:910	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	65	dep	microbes	727:734	arg1	microbes					727:734	intestinal microbes	716:734	intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces)	716:1302	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	65	dep	microbes	727:734	arg1	Ruminococcus					992:1003	Ruminococcus	992:1003	Ruminococcus	992:1003	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	65	dep	microbes	727:734	arg1	Butyricicoccus					1009:1022	Butyricicoccus	1009:1022	Butyricicoccus	1009:1022	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	65	dep	microbes	727:734	arg1	XlVb					925:928	Clostridium XlVb	913:928	Clostridium XlVb	913:928	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	65	dep	microbes	727:734	arg1	Butyricimonas					958:970	Butyricimonas	958:970	Butyricimonas	958:970	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	65	dep	microbes	727:734	arg1	Lactobacillus					1105:1117	Lactobacillus	1105:1117	Lactobacillus	1105:1117	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	1	66	theme	plant	210:214	arg1	TFPS					217:220	tea (Camellia sinensis L.) plant (TFPS)	183:221	tea (Camellia sinensis L.) plant (TFPS)	183:221	Polysaccharides from the flowers of tea (Camellia sinensis L.) plant (TFPS) were considered as potential functional components mainly playing the role in the distal intestines.
32371134	4	67	dep	7.61 ± 0.81 mM	1433:1446	arg1	to					1430:1431	to	1430:1431	to	1430:1431	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	68	theme	simulated	628:636	arg1	conditions					648:657	simulated anaerobic conditions	628:657	simulated anaerobic conditions	628:657	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	0	69	with	patients	105:112	arg1	disease					138:144	inflammatory bowel disease	119:144	inflammatory bowel disease	119:144	Prebiotics effects in vitro of polysaccharides from tea flowers on gut microbiota of healthy persons and patients with inflammatory bowel disease.
32371134	4	70	theme	IBD	1456:1458	arg1	feces					1460:1464	the IBD feces	1452:1464	the IBD feces	1452:1464	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	2	71	theme	bowel	457:461	arg1	IBD					472:474	IBD	472:474	IBD	472:474	In the present study, effects of TFPS on compositions of gut microbiota of feces from healthy persons and patients with inflammatory bowel disease (IBD) were investigated in vitro.
32371134	2	71	theme	bowel	457:461	arg1	disease					463:469	inflammatory bowel disease	444:469	inflammatory bowel disease (IBD)	444:475	In the present study, effects of TFPS on compositions of gut microbiota of feces from healthy persons and patients with inflammatory bowel disease (IBD) were investigated in vitro.
32371134	4	72	theme	fatty	1339:1343	arg1	microbes					727:734	intestinal microbes	716:734	intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces)	716:1302	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	4	72	theme	fatty	1339:1343	arg1	acids					1345:1349	short-chain fatty acids	1327:1349	short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces)	1327:1465	After fermentation of TFPS for 24 h under simulated anaerobic conditions, significant changes were observed in the composition of intestinal microbes (enhanced the relative abundances of Klebsiella, Dialister, Megasphaera, Collinsella, Lactobacillus and Bifidobacterium while decreased Prevotella, Clostridium XlVa, Alistipes, Clostridium XlVb, Akkermansia, Ruminococcus, Butyricimonas, Clostridium XVIII, Ruminococcus and Butyricicoccus for the normal feces; increased Escherichia/Shigella, Enterococcus, Collinsella, Lactobacillus and Bifidobacterium while decreased Enterobacter, Streptococcus, Bacteroides, Clostridium XlVa, Megasphaera, Roseburia, Granulicatella, Akkermansia and Fusobacterium for the IBD feces) and their metabolites, short-chain fatty acids (from 4.54 ± 0.43 to 12.88 ± 1.59 mM for the normal feces and from 5.21 ± 0.51 to 7.61 ± 0.81 mM for the IBD feces).
32371134	1	73	dep	Camellia	188:195	arg1	L.					206:207	Camellia sinensis L.	188:207	Camellia sinensis L.	188:207	Polysaccharides from the flowers of tea (Camellia sinensis L.) plant (TFPS) were considered as potential functional components mainly playing the role in the distal intestines.
32371134	2	74	with	patients	430:437	arg1	IBD					472:474	IBD	472:474	IBD	472:474	In the present study, effects of TFPS on compositions of gut microbiota of feces from healthy persons and patients with inflammatory bowel disease (IBD) were investigated in vitro.
32371134	2	74	with	patients	430:437	arg1	disease					463:469	inflammatory bowel disease	444:469	inflammatory bowel disease (IBD)	444:475	In the present study, effects of TFPS on compositions of gut microbiota of feces from healthy persons and patients with inflammatory bowel disease (IBD) were investigated in vitro.
32371134	2	75	theme	present	331:337	arg1	study					339:343	the present study	327:343	the present study	327:343	In the present study, effects of TFPS on compositions of gut microbiota of feces from healthy persons and patients with inflammatory bowel disease (IBD) were investigated in vitro.
32371134	1	76	theme	TFPS	217:220	arg1	flowers					172:178	the flowers	168:178	the flowers of tea (Camellia sinensis L.) plant (TFPS)	168:221	Polysaccharides from the flowers of tea (Camellia sinensis L.) plant (TFPS) were considered as potential functional components mainly playing the role in the distal intestines.
33684807	9	0	theme	count	1456:1460	arg1	levels					1429:1434	lower levels	1423:1434	lower levels of the total viable count, coliform, mold, yeasts, and higher sensory properties	1423:1515	Another observation was that lower levels of the total viable count, coliform, mold, yeasts, and higher sensory properties were significantly associated with samples with added ɛ-PL (0.9%).
33684807	6	1	theme	higher	931:936	arg1	values					938:943	higher values	931:943	higher values of thickness and tensile strength	931:977	In addition, higher values of thickness and tensile strength were correlated with ɛ-PL added active chitosan films while compared with control samples.
33684807	1	2	theme	active	214:219	arg1	%					237:237	1%	236:237	1%	236:237	In the current study, the effect on packaged beef fillets (1 × 5 × 8 cm) of using active chitosan film (1%) was investigated.
33684807	1	2	theme	active	214:219	arg1	film					230:233	active chitosan film	214:233	active chitosan film (1%)	214:238	In the current study, the effect on packaged beef fillets (1 × 5 × 8 cm) of using active chitosan film (1%) was investigated.
33684807	9	3	theme	coliform	1463:1470	arg1	levels					1429:1434	lower levels	1423:1434	lower levels of the total viable count, coliform, mold, yeasts, and higher sensory properties	1423:1515	Another observation was that lower levels of the total viable count, coliform, mold, yeasts, and higher sensory properties were significantly associated with samples with added ɛ-PL (0.9%).
33684807	3	4	theme	quality	423:429	arg1	indices					431:437	quality indices	423:437	quality indices	423:437	Chemical, microbiological, sensory properties, and quality indices of the fillets were investigated.
33684807	9	5	theme	mold	1473:1476	arg1	levels					1429:1434	lower levels	1423:1434	lower levels of the total viable count, coliform, mold, yeasts, and higher sensory properties	1423:1515	Another observation was that lower levels of the total viable count, coliform, mold, yeasts, and higher sensory properties were significantly associated with samples with added ɛ-PL (0.9%).
33684807	6	6	theme	active	1011:1016	arg1	films					1027:1031	active chitosan films	1011:1031	active chitosan films	1011:1031	In addition, higher values of thickness and tensile strength were correlated with ɛ-PL added active chitosan films while compared with control samples.
33684807	10	7	theme	beef	1701:1704	arg1	fillets					1706:1712	beef fillets	1701:1712	beef fillets	1701:1712	Therefore, adding ɛ-PL into chitosan films could be introduced as an effective technique to extend the shelf life of beef fillets and maintain their quality indices during refrigerated storage.
33684807	5	8	theme	significant	715:725	arg1	difference					727:736	a significant difference	713:736	a significant difference among the corresponding values to thickness, color, water vapor permeability (WVP), and mechanical specifications between the treated films by ɛ-PL and untreated films	713:904	Based on the findings, a significant difference among the corresponding values to thickness, color, water vapor permeability (WVP), and mechanical specifications between the treated films by ɛ-PL and untreated films were noted.
33684807	9	9	theme	yeasts	1479:1484	arg1	levels					1429:1434	lower levels	1423:1434	lower levels of the total viable count, coliform, mold, yeasts, and higher sensory properties	1423:1515	Another observation was that lower levels of the total viable count, coliform, mold, yeasts, and higher sensory properties were significantly associated with samples with added ɛ-PL (0.9%).
33684807	4	10	theme	incorporation	547:559	arg1	influence					521:529	the influence	517:529	the influence of ɛ-polylysine incorporation on the optical, structural, barrier, and mechanical specifications (elongation at break and tensile strength) of chitosan films	517:687	Added to these factors was an assessment of the influence of ɛ-polylysine incorporation on the optical, structural, barrier, and mechanical specifications (elongation at break and tensile strength) of chitosan films.
33684807	3	11	theme	fillets	446:452	arg1	properties					407:416	sensory properties	399:416	sensory properties	399:416	Chemical, microbiological, sensory properties, and quality indices of the fillets were investigated.
33684807	3	11	theme	fillets	446:452	arg1	indices					431:437	quality indices	423:437	quality indices	423:437	Chemical, microbiological, sensory properties, and quality indices of the fillets were investigated.
33684807	8	12	from	levels	1270:1275	arg1	films					1318:1322	enriched films	1309:1322	enriched films	1309:1322	In this regard, due to significantly lower levels of pH, TVB-N, and TBARS ɛ-PL in enriched films, this technique demonstrated some protective effects on beef fillets.
33684807	0	13	theme	indices	108:114	arg1	assessment					86:95	An assessment	83:95	An assessment of quality indices and shelf life	83:129	Packaging of beef fillet with active chitosan film incorporated with ɛ-polylysine: An assessment of quality indices and shelf life.
33684807	4	14	from	assessment	503:512	arg1	specifications					613:626	the optical, structural, barrier, and mechanical specifications	564:626	the optical, structural, barrier, and mechanical specifications (elongation at break and tensile strength) of chitosan films	564:687	Added to these factors was an assessment of the influence of ɛ-polylysine incorporation on the optical, structural, barrier, and mechanical specifications (elongation at break and tensile strength) of chitosan films.
33684807	1	15	theme	packaged	168:175	arg1	1 × 5 × 8 cm					191:202	1 × 5 × 8 cm	191:202	1 × 5 × 8 cm	191:202	In the current study, the effect on packaged beef fillets (1 × 5 × 8 cm) of using active chitosan film (1%) was investigated.
33684807	1	15	theme	packaged	168:175	arg1	fillets					182:188	packaged beef fillets	168:188	packaged beef fillets (1 × 5 × 8 cm)	168:203	In the current study, the effect on packaged beef fillets (1 × 5 × 8 cm) of using active chitosan film (1%) was investigated.
33684807	9	16	theme	sensory	1498:1504	arg1	properties					1506:1515	higher sensory properties	1491:1515	higher sensory properties	1491:1515	Another observation was that lower levels of the total viable count, coliform, mold, yeasts, and higher sensory properties were significantly associated with samples with added ɛ-PL (0.9%).
33684807	0	17	theme	shelf	120:124	arg1	life					126:129	shelf life	120:129	shelf life	120:129	Packaging of beef fillet with active chitosan film incorporated with ɛ-polylysine: An assessment of quality indices and shelf life.
33684807	10	18	theme	refrigerated	1756:1767	arg1	storage					1769:1775	refrigerated storage	1756:1775	refrigerated storage	1756:1775	Therefore, adding ɛ-PL into chitosan films could be introduced as an effective technique to extend the shelf life of beef fillets and maintain their quality indices during refrigerated storage.
33684807	4	19	dep	specifications	613:626	arg1	elongation					629:638	elongation	629:638	elongation	629:638	Added to these factors was an assessment of the influence of ɛ-polylysine incorporation on the optical, structural, barrier, and mechanical specifications (elongation at break and tensile strength) of chitosan films.
33684807	7	20	theme	fillet	1197:1202	arg1	samples					1204:1210	beef fillet samples	1192:1210	beef fillet samples	1192:1210	Additionally, no significant differences regarding the proximate composition (including protein, moisture, and fat) among beef fillet samples were observed.
33684807	6	21	theme	control	1053:1059	arg1	samples					1061:1067	control samples	1053:1067	control samples	1053:1067	In addition, higher values of thickness and tensile strength were correlated with ɛ-PL added active chitosan films while compared with control samples.
33684807	10	22	theme	chitosan	1612:1619	arg1	films					1621:1625	chitosan films	1612:1625	chitosan films	1612:1625	Therefore, adding ɛ-PL into chitosan films could be introduced as an effective technique to extend the shelf life of beef fillets and maintain their quality indices during refrigerated storage.
33684807	8	23	theme	TBARS	1295:1299	arg1	levels					1270:1275	significantly lower levels	1250:1275	significantly lower levels of pH, TVB-N, and TBARS ɛ-PL in enriched films	1250:1322	In this regard, due to significantly lower levels of pH, TVB-N, and TBARS ɛ-PL in enriched films, this technique demonstrated some protective effects on beef fillets.
33684807	4	24	from	strength	661:668	arg1	elongation					629:638	elongation	629:638	elongation	629:638	Added to these factors was an assessment of the influence of ɛ-polylysine incorporation on the optical, structural, barrier, and mechanical specifications (elongation at break and tensile strength) of chitosan films.
33684807	5	25	theme	corresponding	748:760	arg1	values					762:767	the corresponding values	744:767	the corresponding values	744:767	Based on the findings, a significant difference among the corresponding values to thickness, color, water vapor permeability (WVP), and mechanical specifications between the treated films by ɛ-PL and untreated films were noted.
33684807	9	26	theme	added	1565:1569	arg1	ɛ-PL					1571:1574	added ɛ-PL	1565:1574	added ɛ-PL (0.9%)	1565:1581	Another observation was that lower levels of the total viable count, coliform, mold, yeasts, and higher sensory properties were significantly associated with samples with added ɛ-PL (0.9%).
33684807	9	26	theme	added	1565:1569	arg1	%					1580:1580	0.9%	1577:1580	0.9%	1577:1580	Another observation was that lower levels of the total viable count, coliform, mold, yeasts, and higher sensory properties were significantly associated with samples with added ɛ-PL (0.9%).
33684807	1	27	theme	current	139:145	arg1	study					147:151	the current study	135:151	the current study	135:151	In the current study, the effect on packaged beef fillets (1 × 5 × 8 cm) of using active chitosan film (1%) was investigated.
33684807	8	28	from	TBARS	1295:1299	arg1	films					1318:1322	enriched films	1309:1322	enriched films	1309:1322	In this regard, due to significantly lower levels of pH, TVB-N, and TBARS ɛ-PL in enriched films, this technique demonstrated some protective effects on beef fillets.
33684807	3	29	dep	Chemical	372:379	arg1	microbiological					382:396	microbiological	382:396	microbiological	382:396	Chemical, microbiological, sensory properties, and quality indices of the fillets were investigated.
33684807	4	30	theme	mechanical	602:611	arg1	specifications					613:626	the optical, structural, barrier, and mechanical specifications	564:626	the optical, structural, barrier, and mechanical specifications (elongation at break and tensile strength) of chitosan films	564:687	Added to these factors was an assessment of the influence of ɛ-polylysine incorporation on the optical, structural, barrier, and mechanical specifications (elongation at break and tensile strength) of chitosan films.
33684807	8	31	from	films	1318:1322	arg1	levels					1270:1275	significantly lower levels	1250:1275	significantly lower levels of pH, TVB-N, and TBARS ɛ-PL in enriched films	1250:1322	In this regard, due to significantly lower levels of pH, TVB-N, and TBARS ɛ-PL in enriched films, this technique demonstrated some protective effects on beef fillets.
33684807	5	32	theme	vapor	796:800	arg1	permeability					802:813	water vapor permeability	790:813	water vapor permeability (WVP)	790:819	Based on the findings, a significant difference among the corresponding values to thickness, color, water vapor permeability (WVP), and mechanical specifications between the treated films by ɛ-PL and untreated films were noted.
33684807	5	32	theme	vapor	796:800	arg1	WVP					816:818	WVP	816:818	WVP	816:818	Based on the findings, a significant difference among the corresponding values to thickness, color, water vapor permeability (WVP), and mechanical specifications between the treated films by ɛ-PL and untreated films were noted.
33684807	8	33	theme	protective	1358:1367	arg1	effects					1369:1375	some protective effects	1353:1375	some protective effects on beef fillets	1353:1391	In this regard, due to significantly lower levels of pH, TVB-N, and TBARS ɛ-PL in enriched films, this technique demonstrated some protective effects on beef fillets.
33684807	0	34	theme	beef	13:16	arg1	fillet					18:23	beef fillet	13:23	beef fillet	13:23	Packaging of beef fillet with active chitosan film incorporated with ɛ-polylysine: An assessment of quality indices and shelf life.
33684807	0	35	dep	incorporated	51:62	arg1	assessment					86:95	An assessment	83:95	An assessment of quality indices and shelf life	83:129	Packaging of beef fillet with active chitosan film incorporated with ɛ-polylysine: An assessment of quality indices and shelf life.
33684807	4	36	theme	films	683:687	arg1	specifications					613:626	the optical, structural, barrier, and mechanical specifications	564:626	the optical, structural, barrier, and mechanical specifications (elongation at break and tensile strength) of chitosan films	564:687	Added to these factors was an assessment of the influence of ɛ-polylysine incorporation on the optical, structural, barrier, and mechanical specifications (elongation at break and tensile strength) of chitosan films.
33684807	8	37	dep	pH	1280:1281	arg1	ɛ-PL					1301:1304	ɛ-PL	1301:1304	ɛ-PL	1301:1304	In this regard, due to significantly lower levels of pH, TVB-N, and TBARS ɛ-PL in enriched films, this technique demonstrated some protective effects on beef fillets.
33684807	6	38	theme	tensile	962:968	arg1	strength					970:977	tensile strength	962:977	tensile strength	962:977	In addition, higher values of thickness and tensile strength were correlated with ɛ-PL added active chitosan films while compared with control samples.
33684807	0	39	with	Packaging	0:8	arg1	chitosan					37:44	active chitosan film	30:49	active chitosan film	30:49	Packaging of beef fillet with active chitosan film incorporated with ɛ-polylysine: An assessment of quality indices and shelf life.
33684807	7	40	theme	proximate	1125:1133	arg1	composition					1135:1145	the proximate composition	1121:1145	the proximate composition (including protein, moisture, and fat)	1121:1184	Additionally, no significant differences regarding the proximate composition (including protein, moisture, and fat) among beef fillet samples were observed.
33684807	2	41	dep	contained	316:324	arg1	%					364:364	0.3, 0.6, and 0.9%	347:364	0.3, 0.6, and 0.9% w/w	347:368	The fillets were stored at 4 °C for 12 days, and the film contained ɛ-polylysine (ɛ-PL) (0.3, 0.6, and 0.9% w/w).
33684807	9	42	theme	viable	1449:1454	arg1	count					1456:1460	the total viable count	1439:1460	the total viable count	1439:1460	Another observation was that lower levels of the total viable count, coliform, mold, yeasts, and higher sensory properties were significantly associated with samples with added ɛ-PL (0.9%).
33684807	10	43	theme	shelf	1687:1691	arg1	life					1693:1696	the shelf life	1683:1696	the shelf life of beef fillets	1683:1712	Therefore, adding ɛ-PL into chitosan films could be introduced as an effective technique to extend the shelf life of beef fillets and maintain their quality indices during refrigerated storage.
33684807	7	44	theme	significant	1087:1097	arg1	differences					1099:1109	no significant differences	1084:1109	no significant differences regarding the proximate composition (including protein, moisture, and fat) among beef fillet samples	1084:1210	Additionally, no significant differences regarding the proximate composition (including protein, moisture, and fat) among beef fillet samples were observed.
33684807	8	45	from	effects	1369:1375	arg1	fillets					1385:1391	beef fillets	1380:1391	beef fillets	1380:1391	In this regard, due to significantly lower levels of pH, TVB-N, and TBARS ɛ-PL in enriched films, this technique demonstrated some protective effects on beef fillets.
33684807	1	46	theme	chitosan	221:228	arg1	%					237:237	1%	236:237	1%	236:237	In the current study, the effect on packaged beef fillets (1 × 5 × 8 cm) of using active chitosan film (1%) was investigated.
33684807	1	46	theme	chitosan	221:228	arg1	film					230:233	active chitosan film	214:233	active chitosan film (1%)	214:238	In the current study, the effect on packaged beef fillets (1 × 5 × 8 cm) of using active chitosan film (1%) was investigated.
33684807	6	47	theme	chitosan	1018:1025	arg1	films					1027:1031	active chitosan films	1011:1031	active chitosan films	1011:1031	In addition, higher values of thickness and tensile strength were correlated with ɛ-PL added active chitosan films while compared with control samples.
33684807	10	48	theme	fillets	1706:1712	arg1	life					1693:1696	the shelf life	1683:1696	the shelf life of beef fillets	1683:1712	Therefore, adding ɛ-PL into chitosan films could be introduced as an effective technique to extend the shelf life of beef fillets and maintain their quality indices during refrigerated storage.
33684807	4	49	theme	influence	521:529	arg1	assessment					503:512	an assessment	500:512	an assessment of the influence of ɛ-polylysine incorporation on the optical, structural, barrier, and mechanical specifications (elongation at break and tensile strength) of chitosan films	500:687	Added to these factors was an assessment of the influence of ɛ-polylysine incorporation on the optical, structural, barrier, and mechanical specifications (elongation at break and tensile strength) of chitosan films.
33684807	4	50	from	influence	521:529	arg1	specifications					613:626	the optical, structural, barrier, and mechanical specifications	564:626	the optical, structural, barrier, and mechanical specifications (elongation at break and tensile strength) of chitosan films	564:687	Added to these factors was an assessment of the influence of ɛ-polylysine incorporation on the optical, structural, barrier, and mechanical specifications (elongation at break and tensile strength) of chitosan films.
33684807	4	51	theme	ɛ-polylysine	534:545	arg1	incorporation					547:559	ɛ-polylysine incorporation	534:559	ɛ-polylysine incorporation	534:559	Added to these factors was an assessment of the influence of ɛ-polylysine incorporation on the optical, structural, barrier, and mechanical specifications (elongation at break and tensile strength) of chitosan films.
33684807	10	52	theme	quality	1733:1739	arg1	indices					1741:1747	their quality indices	1727:1747	their quality indices	1727:1747	Therefore, adding ɛ-PL into chitosan films could be introduced as an effective technique to extend the shelf life of beef fillets and maintain their quality indices during refrigerated storage.
33684807	9	53	theme	higher	1491:1496	arg1	properties					1506:1515	higher sensory properties	1491:1515	higher sensory properties	1491:1515	Another observation was that lower levels of the total viable count, coliform, mold, yeasts, and higher sensory properties were significantly associated with samples with added ɛ-PL (0.9%).
33684807	8	54	theme	pH	1280:1281	arg1	levels					1270:1275	significantly lower levels	1250:1275	significantly lower levels of pH, TVB-N, and TBARS ɛ-PL in enriched films	1250:1322	In this regard, due to significantly lower levels of pH, TVB-N, and TBARS ɛ-PL in enriched films, this technique demonstrated some protective effects on beef fillets.
33684807	0	55	theme	life	126:129	arg1	assessment					86:95	An assessment	83:95	An assessment of quality indices and shelf life	83:129	Packaging of beef fillet with active chitosan film incorporated with ɛ-polylysine: An assessment of quality indices and shelf life.
33684807	10	56	theme	adding	1595:1600	arg1	technique					1663:1671	an effective technique	1650:1671	an effective technique to extend the shelf life of beef fillets and maintain their quality indices during refrigerated storage	1650:1775	Therefore, adding ɛ-PL into chitosan films could be introduced as an effective technique to extend the shelf life of beef fillets and maintain their quality indices during refrigerated storage.
33684807	10	56	theme	adding	1595:1600	arg1	ɛ-PL					1602:1605	adding ɛ-PL	1595:1605	adding ɛ-PL into chitosan films	1595:1625	Therefore, adding ɛ-PL into chitosan films could be introduced as an effective technique to extend the shelf life of beef fillets and maintain their quality indices during refrigerated storage.
33684807	1	57	theme	beef	177:180	arg1	1 × 5 × 8 cm					191:202	1 × 5 × 8 cm	191:202	1 × 5 × 8 cm	191:202	In the current study, the effect on packaged beef fillets (1 × 5 × 8 cm) of using active chitosan film (1%) was investigated.
33684807	1	57	theme	beef	177:180	arg1	fillets					182:188	packaged beef fillets	168:188	packaged beef fillets (1 × 5 × 8 cm)	168:203	In the current study, the effect on packaged beef fillets (1 × 5 × 8 cm) of using active chitosan film (1%) was investigated.
33684807	9	58	theme	properties	1506:1515	arg1	levels					1429:1434	lower levels	1423:1434	lower levels of the total viable count, coliform, mold, yeasts, and higher sensory properties	1423:1515	Another observation was that lower levels of the total viable count, coliform, mold, yeasts, and higher sensory properties were significantly associated with samples with added ɛ-PL (0.9%).
33684807	8	59	theme	TVB-N	1284:1288	arg1	levels					1270:1275	significantly lower levels	1250:1275	significantly lower levels of pH, TVB-N, and TBARS ɛ-PL in enriched films	1250:1322	In this regard, due to significantly lower levels of pH, TVB-N, and TBARS ɛ-PL in enriched films, this technique demonstrated some protective effects on beef fillets.
33684807	2	60	contain	contained	316:324	arg1	film					311:314	the film	307:314	the film	307:314	The fillets were stored at 4 °C for 12 days, and the film contained ɛ-polylysine (ɛ-PL) (0.3, 0.6, and 0.9% w/w).
33684807	2	60	contain	contained	316:324	arg2	ɛ-PL					340:343	ɛ-PL	340:343	ɛ-PL	340:343	The fillets were stored at 4 °C for 12 days, and the film contained ɛ-polylysine (ɛ-PL) (0.3, 0.6, and 0.9% w/w).
33684807	2	60	contain	contained	316:324	arg2	ɛ-polylysine					326:337	ɛ-polylysine	326:337	ɛ-polylysine (ɛ-PL)	326:344	The fillets were stored at 4 °C for 12 days, and the film contained ɛ-polylysine (ɛ-PL) (0.3, 0.6, and 0.9% w/w).
33684807	4	61	theme	structural	577:586	arg1	specifications					613:626	the optical, structural, barrier, and mechanical specifications	564:626	the optical, structural, barrier, and mechanical specifications (elongation at break and tensile strength) of chitosan films	564:687	Added to these factors was an assessment of the influence of ɛ-polylysine incorporation on the optical, structural, barrier, and mechanical specifications (elongation at break and tensile strength) of chitosan films.
33684807	5	62	theme	untreated	890:898	arg1	films					900:904	untreated films	890:904	untreated films	890:904	Based on the findings, a significant difference among the corresponding values to thickness, color, water vapor permeability (WVP), and mechanical specifications between the treated films by ɛ-PL and untreated films were noted.
33684807	4	63	theme	barrier	589:595	arg1	specifications					613:626	the optical, structural, barrier, and mechanical specifications	564:626	the optical, structural, barrier, and mechanical specifications (elongation at break and tensile strength) of chitosan films	564:687	Added to these factors was an assessment of the influence of ɛ-polylysine incorporation on the optical, structural, barrier, and mechanical specifications (elongation at break and tensile strength) of chitosan films.
33684807	8	64	from	TVB-N	1284:1288	arg1	films					1318:1322	enriched films	1309:1322	enriched films	1309:1322	In this regard, due to significantly lower levels of pH, TVB-N, and TBARS ɛ-PL in enriched films, this technique demonstrated some protective effects on beef fillets.
33684807	9	65	with	samples	1552:1558	arg1	ɛ-PL					1571:1574	added ɛ-PL	1565:1574	added ɛ-PL (0.9%)	1565:1581	Another observation was that lower levels of the total viable count, coliform, mold, yeasts, and higher sensory properties were significantly associated with samples with added ɛ-PL (0.9%).
33684807	9	65	with	samples	1552:1558	arg1	%					1580:1580	0.9%	1577:1580	0.9%	1577:1580	Another observation was that lower levels of the total viable count, coliform, mold, yeasts, and higher sensory properties were significantly associated with samples with added ɛ-PL (0.9%).
33684807	1	66	from	effect	158:163	arg1	1 × 5 × 8 cm					191:202	1 × 5 × 8 cm	191:202	1 × 5 × 8 cm	191:202	In the current study, the effect on packaged beef fillets (1 × 5 × 8 cm) of using active chitosan film (1%) was investigated.
33684807	1	66	from	effect	158:163	arg1	fillets					182:188	packaged beef fillets	168:188	packaged beef fillets (1 × 5 × 8 cm)	168:203	In the current study, the effect on packaged beef fillets (1 × 5 × 8 cm) of using active chitosan film (1%) was investigated.
33684807	8	67	theme	enriched	1309:1316	arg1	films					1318:1322	enriched films	1309:1322	enriched films	1309:1322	In this regard, due to significantly lower levels of pH, TVB-N, and TBARS ɛ-PL in enriched films, this technique demonstrated some protective effects on beef fillets.
33684807	7	68	theme	beef	1192:1195	arg1	samples					1204:1210	beef fillet samples	1192:1210	beef fillet samples	1192:1210	Additionally, no significant differences regarding the proximate composition (including protein, moisture, and fat) among beef fillet samples were observed.
33684807	8	69	from	pH	1280:1281	arg1	films					1318:1322	enriched films	1309:1322	enriched films	1309:1322	In this regard, due to significantly lower levels of pH, TVB-N, and TBARS ɛ-PL in enriched films, this technique demonstrated some protective effects on beef fillets.
33684807	4	70	theme	optical	568:574	arg1	specifications					613:626	the optical, structural, barrier, and mechanical specifications	564:626	the optical, structural, barrier, and mechanical specifications (elongation at break and tensile strength) of chitosan films	564:687	Added to these factors was an assessment of the influence of ɛ-polylysine incorporation on the optical, structural, barrier, and mechanical specifications (elongation at break and tensile strength) of chitosan films.
33684807	2	71	dep	%	364:364	arg1	w/w					366:368	w/w	366:368	0.3, 0.6, and 0.9% w/w	347:368	The fillets were stored at 4 °C for 12 days, and the film contained ɛ-polylysine (ɛ-PL) (0.3, 0.6, and 0.9% w/w).
33684807	4	72	from	break	643:647	arg1	elongation					629:638	elongation	629:638	elongation	629:638	Added to these factors was an assessment of the influence of ɛ-polylysine incorporation on the optical, structural, barrier, and mechanical specifications (elongation at break and tensile strength) of chitosan films.
33684807	6	73	theme	thickness	948:956	arg1	values					938:943	higher values	931:943	higher values of thickness and tensile strength	931:977	In addition, higher values of thickness and tensile strength were correlated with ɛ-PL added active chitosan films while compared with control samples.
33684807	0	74	theme	quality	100:106	arg1	indices					108:114	quality indices	100:114	quality indices	100:114	Packaging of beef fillet with active chitosan film incorporated with ɛ-polylysine: An assessment of quality indices and shelf life.
33684807	4	75	from	specifications	613:626	arg1	assessment					503:512	an assessment	500:512	an assessment of the influence of ɛ-polylysine incorporation on the optical, structural, barrier, and mechanical specifications (elongation at break and tensile strength) of chitosan films	500:687	Added to these factors was an assessment of the influence of ɛ-polylysine incorporation on the optical, structural, barrier, and mechanical specifications (elongation at break and tensile strength) of chitosan films.
33684807	5	76	theme	water	790:794	arg1	permeability					802:813	water vapor permeability	790:813	water vapor permeability (WVP)	790:819	Based on the findings, a significant difference among the corresponding values to thickness, color, water vapor permeability (WVP), and mechanical specifications between the treated films by ɛ-PL and untreated films were noted.
33684807	5	76	theme	water	790:794	arg1	WVP					816:818	WVP	816:818	WVP	816:818	Based on the findings, a significant difference among the corresponding values to thickness, color, water vapor permeability (WVP), and mechanical specifications between the treated films by ɛ-PL and untreated films were noted.
33684807	0	77	theme	fillet	18:23	arg1	Packaging					0:8	Packaging	0:8	Packaging of beef fillet with active chitosan film	0:49	Packaging of beef fillet with active chitosan film incorporated with ɛ-polylysine: An assessment of quality indices and shelf life.
33684807	8	78	theme	beef	1380:1383	arg1	fillets					1385:1391	beef fillets	1380:1391	beef fillets	1380:1391	In this regard, due to significantly lower levels of pH, TVB-N, and TBARS ɛ-PL in enriched films, this technique demonstrated some protective effects on beef fillets.
33684807	0	79	theme	active	30:35	arg1	chitosan					37:44	active chitosan film	30:49	active chitosan film	30:49	Packaging of beef fillet with active chitosan film incorporated with ɛ-polylysine: An assessment of quality indices and shelf life.
33684807	5	80	theme	treated	864:870	arg1	films					872:876	the treated films	860:876	the treated films by ɛ-PL and untreated films	860:904	Based on the findings, a significant difference among the corresponding values to thickness, color, water vapor permeability (WVP), and mechanical specifications between the treated films by ɛ-PL and untreated films were noted.
33684807	4	81	theme	chitosan	674:681	arg1	films					683:687	chitosan films	674:687	chitosan films	674:687	Added to these factors was an assessment of the influence of ɛ-polylysine incorporation on the optical, structural, barrier, and mechanical specifications (elongation at break and tensile strength) of chitosan films.
33684807	9	82	theme	lower	1423:1427	arg1	levels					1429:1434	lower levels	1423:1434	lower levels of the total viable count, coliform, mold, yeasts, and higher sensory properties	1423:1515	Another observation was that lower levels of the total viable count, coliform, mold, yeasts, and higher sensory properties were significantly associated with samples with added ɛ-PL (0.9%).
33684807	6	83	theme	strength	970:977	arg1	values					938:943	higher values	931:943	higher values of thickness and tensile strength	931:977	In addition, higher values of thickness and tensile strength were correlated with ɛ-PL added active chitosan films while compared with control samples.
33684807	3	84	theme	Chemical	372:379	arg1	properties					407:416	sensory properties	399:416	sensory properties	399:416	Chemical, microbiological, sensory properties, and quality indices of the fillets were investigated.
33684807	5	85	theme	mechanical	826:835	arg1	specifications					837:850	mechanical specifications	826:850	mechanical specifications	826:850	Based on the findings, a significant difference among the corresponding values to thickness, color, water vapor permeability (WVP), and mechanical specifications between the treated films by ɛ-PL and untreated films were noted.
33684807	10	86	theme	effective	1653:1661	arg1	technique					1663:1671	an effective technique	1650:1671	an effective technique to extend the shelf life of beef fillets and maintain their quality indices during refrigerated storage	1650:1775	Therefore, adding ɛ-PL into chitosan films could be introduced as an effective technique to extend the shelf life of beef fillets and maintain their quality indices during refrigerated storage.
33684807	10	86	theme	effective	1653:1661	arg1	ɛ-PL					1602:1605	adding ɛ-PL	1595:1605	adding ɛ-PL into chitosan films	1595:1625	Therefore, adding ɛ-PL into chitosan films could be introduced as an effective technique to extend the shelf life of beef fillets and maintain their quality indices during refrigerated storage.
33684807	4	87	theme	tensile	653:659	arg1	strength					661:668	tensile strength	653:668	tensile strength	653:668	Added to these factors was an assessment of the influence of ɛ-polylysine incorporation on the optical, structural, barrier, and mechanical specifications (elongation at break and tensile strength) of chitosan films.
33684807	9	88	theme	total	1443:1447	arg1	count					1456:1460	the total viable count	1439:1460	the total viable count	1439:1460	Another observation was that lower levels of the total viable count, coliform, mold, yeasts, and higher sensory properties were significantly associated with samples with added ɛ-PL (0.9%).
33684807	8	89	theme	lower	1264:1268	arg1	levels					1270:1275	significantly lower levels	1250:1275	significantly lower levels of pH, TVB-N, and TBARS ɛ-PL in enriched films	1250:1322	In this regard, due to significantly lower levels of pH, TVB-N, and TBARS ɛ-PL in enriched films, this technique demonstrated some protective effects on beef fillets.
33684807	3	90	theme	sensory	399:405	arg1	properties					407:416	sensory properties	399:416	sensory properties	399:416	Chemical, microbiological, sensory properties, and quality indices of the fillets were investigated.
32899660	9	0	theme	PEY	1288:1290	arg1	complexity					1274:1283	the complexity	1270:1283	the complexity of PEY	1270:1290	PEY vs. egg-yolk comparative metabolomic analyses showed significative differences in the concentrations of at least 140 molecules, and in 357 in the lipidomic analyses, demonstrating the complexity of PEY.
32899660	8	1	theme	platelet-derived	1032:1047	arg1	PDGF					1064:1067	PDGF	1064:1067	PDGF	1064:1067	Immunochemical analyses demonstrated increased concentrations of insulin-like growth factor 1 (IGF-1), connective tissue growth factor (CTGF), and platelet-derived growth factor (PDGF) in the extract.
32899660	8	1	theme	platelet-derived	1032:1047	arg1	factor					1056:1061	platelet-derived growth factor	1032:1061	platelet-derived growth factor (PDGF)	1032:1068	Immunochemical analyses demonstrated increased concentrations of insulin-like growth factor 1 (IGF-1), connective tissue growth factor (CTGF), and platelet-derived growth factor (PDGF) in the extract.
32899660	5	2	theme	growth	578:583	arg1	composition					592:602	growth factor composition	578:602	growth factor composition	578:602	We explored growth factor composition, and mass-spectrometry metabolome and lipidome analyses of PEY to characterize it.
32899660	0	3	theme	Yolk	74:77	arg1	Effects					26:32	In Vivo Anti-Inflammatory Effects	0:32	In Vivo Anti-Inflammatory Effects	0:32	In Vivo Anti-Inflammatory Effects and Related Mechanisms of Processed Egg Yolk, a Potential Anti-Inflammaging Dietary Supplement.
32899660	0	3	theme	Yolk	74:77	arg1	Mechanisms					46:55	Related Mechanisms	38:55	Related Mechanisms of Processed Egg Yolk	38:77	In Vivo Anti-Inflammatory Effects and Related Mechanisms of Processed Egg Yolk, a Potential Anti-Inflammaging Dietary Supplement.
32899660	5	4	theme	PEY	663:665	arg1	metabolome					627:636	mass-spectrometry metabolome	609:636	mass-spectrometry metabolome	609:636	We explored growth factor composition, and mass-spectrometry metabolome and lipidome analyses of PEY to characterize it.
32899660	5	4	theme	PEY	663:665	arg1	analyses					651:658	lipidome analyses	642:658	lipidome analyses of PEY	642:665	We explored growth factor composition, and mass-spectrometry metabolome and lipidome analyses of PEY to characterize it.
32899660	5	4	theme	PEY	663:665	arg1	composition					592:602	growth factor composition	578:602	growth factor composition	578:602	We explored growth factor composition, and mass-spectrometry metabolome and lipidome analyses of PEY to characterize it.
32899660	8	5	theme	tissue	999:1004	arg1	CTGF					1021:1024	CTGF	1021:1024	CTGF	1021:1024	Immunochemical analyses demonstrated increased concentrations of insulin-like growth factor 1 (IGF-1), connective tissue growth factor (CTGF), and platelet-derived growth factor (PDGF) in the extract.
32899660	8	5	theme	tissue	999:1004	arg1	factor					1013:1018	connective tissue growth factor	988:1018	connective tissue growth factor (CTGF)	988:1025	Immunochemical analyses demonstrated increased concentrations of insulin-like growth factor 1 (IGF-1), connective tissue growth factor (CTGF), and platelet-derived growth factor (PDGF) in the extract.
32899660	0	6	dep	Supplement	118:127	arg1	Effects					26:32	In Vivo Anti-Inflammatory Effects	0:32	In Vivo Anti-Inflammatory Effects	0:32	In Vivo Anti-Inflammatory Effects and Related Mechanisms of Processed Egg Yolk, a Potential Anti-Inflammaging Dietary Supplement.
32899660	0	6	dep	Supplement	118:127	arg1	Mechanisms					46:55	Related Mechanisms	38:55	Related Mechanisms of Processed Egg Yolk	38:77	In Vivo Anti-Inflammatory Effects and Related Mechanisms of Processed Egg Yolk, a Potential Anti-Inflammaging Dietary Supplement.
32899660	10	7	from	interest	1378:1385	arg1	inflammaging					1457:1468	inflammaging	1457:1468	inflammaging	1457:1468	Globally, PEY acts as an orally-bioavailable immunomodulatory extract that may be of interest in those conditions associated with disarranged inflammation, such as inflammaging.
32899660	10	7	from	interest	1378:1385	arg1	conditions					1396:1405	those conditions	1390:1405	those conditions	1390:1405	Globally, PEY acts as an orally-bioavailable immunomodulatory extract that may be of interest in those conditions associated with disarranged inflammation, such as inflammaging.
32899660	0	8	theme	Potential	82:90	arg1	Supplement					118:127	a Potential Anti-Inflammaging Dietary Supplement	80:127	a Potential Anti-Inflammaging Dietary Supplement	80:127	In Vivo Anti-Inflammatory Effects and Related Mechanisms of Processed Egg Yolk, a Potential Anti-Inflammaging Dietary Supplement.
32899660	4	9	link	derived	543:549	arg1	samples					557:563	derived serum samples	543:563	derived serum samples	543:563	Cell culture was used to explore ex vivo properties of derived serum samples.
32899660	8	10	theme	factor	1056:1061	arg1	concentrations					932:945	increased concentrations	922:945	increased concentrations of insulin-like growth factor 1 (IGF-1), connective tissue growth factor (CTGF), and platelet-derived growth factor (PDGF) in the extract	922:1083	Immunochemical analyses demonstrated increased concentrations of insulin-like growth factor 1 (IGF-1), connective tissue growth factor (CTGF), and platelet-derived growth factor (PDGF) in the extract.
32899660	8	11	theme	connective	988:997	arg1	CTGF					1021:1024	CTGF	1021:1024	CTGF	1021:1024	Immunochemical analyses demonstrated increased concentrations of insulin-like growth factor 1 (IGF-1), connective tissue growth factor (CTGF), and platelet-derived growth factor (PDGF) in the extract.
32899660	8	11	theme	connective	988:997	arg1	factor					1013:1018	connective tissue growth factor	988:1018	connective tissue growth factor (CTGF)	988:1025	Immunochemical analyses demonstrated increased concentrations of insulin-like growth factor 1 (IGF-1), connective tissue growth factor (CTGF), and platelet-derived growth factor (PDGF) in the extract.
32899660	6	12	theme	IL-1	739:742	arg1	β					744:744	IL-1 β	739:744	IL-1 β	739:744	PEY significantly prevented LPS-induced increase in IL-1 β, TNF-α, and MCP-1.
32899660	8	13	theme	growth	1049:1054	arg1	PDGF					1064:1067	PDGF	1064:1067	PDGF	1064:1067	Immunochemical analyses demonstrated increased concentrations of insulin-like growth factor 1 (IGF-1), connective tissue growth factor (CTGF), and platelet-derived growth factor (PDGF) in the extract.
32899660	8	13	theme	growth	1049:1054	arg1	factor					1056:1061	platelet-derived growth factor	1032:1061	platelet-derived growth factor (PDGF)	1032:1068	Immunochemical analyses demonstrated increased concentrations of insulin-like growth factor 1 (IGF-1), connective tissue growth factor (CTGF), and platelet-derived growth factor (PDGF) in the extract.
32899660	8	14	theme	factor	1013:1018	arg1	concentrations					932:945	increased concentrations	922:945	increased concentrations of insulin-like growth factor 1 (IGF-1), connective tissue growth factor (CTGF), and platelet-derived growth factor (PDGF) in the extract	922:1083	Immunochemical analyses demonstrated increased concentrations of insulin-like growth factor 1 (IGF-1), connective tissue growth factor (CTGF), and platelet-derived growth factor (PDGF) in the extract.
32899660	9	15	theme	comparative	1103:1113	arg1	analyses					1127:1134	PEY vs. egg-yolk comparative metabolomic analyses	1086:1134	PEY vs. egg-yolk comparative metabolomic analyses	1086:1134	PEY vs. egg-yolk comparative metabolomic analyses showed significative differences in the concentrations of at least 140 molecules, and in 357 in the lipidomic analyses, demonstrating the complexity of PEY.
32899660	7	16	theme	macrophage-like	858:872	arg1	line					879:882	macrophage-like cell line	858:882	the Raw 264.7 macrophage-like cell line	844:882	Further, serum from PEY-treated animals abrogated LPS-induced iNOS build-up of the Raw 264.7 macrophage-like cell line.
32899660	9	17	theme	PEY	1086:1088	arg1	analyses					1127:1134	PEY vs. egg-yolk comparative metabolomic analyses	1086:1134	PEY vs. egg-yolk comparative metabolomic analyses	1086:1134	PEY vs. egg-yolk comparative metabolomic analyses showed significative differences in the concentrations of at least 140 molecules, and in 357 in the lipidomic analyses, demonstrating the complexity of PEY.
32899660	0	18	theme	Dietary	110:116	arg1	Supplement					118:127	a Potential Anti-Inflammaging Dietary Supplement	80:127	a Potential Anti-Inflammaging Dietary Supplement	80:127	In Vivo Anti-Inflammatory Effects and Related Mechanisms of Processed Egg Yolk, a Potential Anti-Inflammaging Dietary Supplement.
32899660	5	19	theme	mass-spectrometry	609:625	arg1	metabolome					627:636	mass-spectrometry metabolome	609:636	mass-spectrometry metabolome	609:636	We explored growth factor composition, and mass-spectrometry metabolome and lipidome analyses of PEY to characterize it.
32899660	7	20	theme	cell	874:877	arg1	line					879:882	macrophage-like cell line	858:882	the Raw 264.7 macrophage-like cell line	844:882	Further, serum from PEY-treated animals abrogated LPS-induced iNOS build-up of the Raw 264.7 macrophage-like cell line.
32899660	3	21	theme	circulating	421:431	arg1	profiles					442:449	in vivo circulating cytokine profiles	413:449	in vivo circulating cytokine profiles measured by multiplexing techniques	413:485	These were evaluated in a lipopolysaccharide (LPS)-challenge in 1-month male rats by in vivo circulating cytokine profiles measured by multiplexing techniques.
32899660	0	22	theme	Anti-Inflammaging	92:108	arg1	Supplement					118:127	a Potential Anti-Inflammaging Dietary Supplement	80:127	a Potential Anti-Inflammaging Dietary Supplement	80:127	In Vivo Anti-Inflammatory Effects and Related Mechanisms of Processed Egg Yolk, a Potential Anti-Inflammaging Dietary Supplement.
32899660	3	23	from	-challenge	378:387	arg1	rats					405:408	1-month male rats	392:408	1-month male rats	392:408	These were evaluated in a lipopolysaccharide (LPS)-challenge in 1-month male rats by in vivo circulating cytokine profiles measured by multiplexing techniques.
32899660	7	24	from	animals	797:803	arg1	serum					774:778	serum	774:778	serum from PEY-treated animals	774:803	Further, serum from PEY-treated animals abrogated LPS-induced iNOS build-up of the Raw 264.7 macrophage-like cell line.
32899660	8	25	from	concentrations	932:945	arg1	extract					1077:1083	the extract	1073:1083	the extract	1073:1083	Immunochemical analyses demonstrated increased concentrations of insulin-like growth factor 1 (IGF-1), connective tissue growth factor (CTGF), and platelet-derived growth factor (PDGF) in the extract.
32899660	6	26	from	increase	727:734	arg1	TNF-α					747:751	TNF-α	747:751	TNF-α	747:751	PEY significantly prevented LPS-induced increase in IL-1 β, TNF-α, and MCP-1.
32899660	6	26	from	increase	727:734	arg1	MCP-1					758:762	MCP-1	758:762	MCP-1	758:762	PEY significantly prevented LPS-induced increase in IL-1 β, TNF-α, and MCP-1.
32899660	6	26	from	increase	727:734	arg1	β					744:744	IL-1 β	739:744	IL-1 β	739:744	PEY significantly prevented LPS-induced increase in IL-1 β, TNF-α, and MCP-1.
32899660	6	27	theme	LPS-induced	715:725	arg1	increase					727:734	LPS-induced increase	715:734	LPS-induced increase in IL-1 β, TNF-α, and MCP-1	715:762	PEY significantly prevented LPS-induced increase in IL-1 β, TNF-α, and MCP-1.
32899660	3	28	theme	cytokine	433:440	arg1	profiles					442:449	in vivo circulating cytokine profiles	413:449	in vivo circulating cytokine profiles measured by multiplexing techniques	413:485	These were evaluated in a lipopolysaccharide (LPS)-challenge in 1-month male rats by in vivo circulating cytokine profiles measured by multiplexing techniques.
32899660	4	29	theme	Cell	488:491	arg1	culture					493:499	Cell culture	488:499	Cell culture	488:499	Cell culture was used to explore ex vivo properties of derived serum samples.
32899660	5	30	theme	lipidome	642:649	arg1	analyses					651:658	lipidome analyses	642:658	lipidome analyses of PEY	642:665	We explored growth factor composition, and mass-spectrometry metabolome and lipidome analyses of PEY to characterize it.
32899660	10	31	theme	disarranged	1423:1433	arg1	inflammation					1435:1446	disarranged inflammation	1423:1446	disarranged inflammation	1423:1446	Globally, PEY acts as an orally-bioavailable immunomodulatory extract that may be of interest in those conditions associated with disarranged inflammation, such as inflammaging.
32899660	8	32	theme	Immunochemical	885:898	arg1	analyses					900:907	Immunochemical analyses	885:907	Immunochemical analyses	885:907	Immunochemical analyses demonstrated increased concentrations of insulin-like growth factor 1 (IGF-1), connective tissue growth factor (CTGF), and platelet-derived growth factor (PDGF) in the extract.
32899660	3	33	theme	in	413:414	arg1	profiles					442:449	in vivo circulating cytokine profiles	413:449	in vivo circulating cytokine profiles measured by multiplexing techniques	413:485	These were evaluated in a lipopolysaccharide (LPS)-challenge in 1-month male rats by in vivo circulating cytokine profiles measured by multiplexing techniques.
32899660	0	34	theme	In	0:1	arg1	Effects					26:32	In Vivo Anti-Inflammatory Effects	0:32	In Vivo Anti-Inflammatory Effects	0:32	In Vivo Anti-Inflammatory Effects and Related Mechanisms of Processed Egg Yolk, a Potential Anti-Inflammaging Dietary Supplement.
32899660	4	35	theme	serum	551:555	arg1	samples					557:563	derived serum samples	543:563	derived serum samples	543:563	Cell culture was used to explore ex vivo properties of derived serum samples.
32899660	4	36	dep	ex	521:522	arg1	vivo					524:527	vivo	524:527	vivo	524:527	Cell culture was used to explore ex vivo properties of derived serum samples.
32899660	2	37	theme	modulatory	305:314	arg1	properties					316:325	inflammation modulatory properties	292:325	inflammation modulatory properties	292:325	We have developed a novel processed egg-yolk (PEY) complement, and we have tested whether it has inflammation modulatory properties.
32899660	8	38	theme	increased	922:930	arg1	concentrations					932:945	increased concentrations	922:945	increased concentrations of insulin-like growth factor 1 (IGF-1), connective tissue growth factor (CTGF), and platelet-derived growth factor (PDGF) in the extract	922:1083	Immunochemical analyses demonstrated increased concentrations of insulin-like growth factor 1 (IGF-1), connective tissue growth factor (CTGF), and platelet-derived growth factor (PDGF) in the extract.
32899660	4	39	theme	derived	543:549	arg1	samples					557:563	derived serum samples	543:563	derived serum samples	543:563	Cell culture was used to explore ex vivo properties of derived serum samples.
32899660	5	40	theme	factor	585:590	arg1	composition					592:602	growth factor composition	578:602	growth factor composition	578:602	We explored growth factor composition, and mass-spectrometry metabolome and lipidome analyses of PEY to characterize it.
32899660	2	41	theme	inflammation	292:303	arg1	properties					316:325	inflammation modulatory properties	292:325	inflammation modulatory properties	292:325	We have developed a novel processed egg-yolk (PEY) complement, and we have tested whether it has inflammation modulatory properties.
32899660	9	42	theme	egg-yolk	1094:1101	arg1	analyses					1127:1134	PEY vs. egg-yolk comparative metabolomic analyses	1086:1134	PEY vs. egg-yolk comparative metabolomic analyses	1086:1134	PEY vs. egg-yolk comparative metabolomic analyses showed significative differences in the concentrations of at least 140 molecules, and in 357 in the lipidomic analyses, demonstrating the complexity of PEY.
32899660	0	43	theme	Anti-Inflammatory	8:24	arg1	Effects					26:32	In Vivo Anti-Inflammatory Effects	0:32	In Vivo Anti-Inflammatory Effects	0:32	In Vivo Anti-Inflammatory Effects and Related Mechanisms of Processed Egg Yolk, a Potential Anti-Inflammaging Dietary Supplement.
32899660	7	44	theme	PEY-treated	785:795	arg1	animals					797:803	PEY-treated animals	785:803	PEY-treated animals	785:803	Further, serum from PEY-treated animals abrogated LPS-induced iNOS build-up of the Raw 264.7 macrophage-like cell line.
32899660	10	45	theme	orally-bioavailable	1318:1336	arg1	PEY					1303:1305	PEY	1303:1305	PEY	1303:1305	Globally, PEY acts as an orally-bioavailable immunomodulatory extract that may be of interest in those conditions associated with disarranged inflammation, such as inflammaging.
32899660	10	45	theme	orally-bioavailable	1318:1336	arg1	extract					1355:1361	an orally-bioavailable immunomodulatory extract	1315:1361	an orally-bioavailable immunomodulatory extract that may be of interest in those conditions associated with disarranged inflammation, such as inflammaging	1315:1468	Globally, PEY acts as an orally-bioavailable immunomodulatory extract that may be of interest in those conditions associated with disarranged inflammation, such as inflammaging.
32899660	1	46	theme	biological	175:184	arg1	effects					186:192	biological effects	175:192	biological effects	175:192	Egg-yolk based supplements have demonstrated biological effects.
32899660	7	47	theme	iNOS	827:830	arg1	build-up					832:839	LPS-induced iNOS build-up	815:839	LPS-induced iNOS build-up of the Raw 264.7 macrophage-like cell line	815:882	Further, serum from PEY-treated animals abrogated LPS-induced iNOS build-up of the Raw 264.7 macrophage-like cell line.
32899660	9	48	from	differences	1157:1167	arg1	concentrations					1176:1189	the concentrations	1172:1189	the concentrations of at least 140 molecules	1172:1215	PEY vs. egg-yolk comparative metabolomic analyses showed significative differences in the concentrations of at least 140 molecules, and in 357 in the lipidomic analyses, demonstrating the complexity of PEY.
32899660	9	48	from	differences	1157:1167	arg1	357					1225:1227	357	1225:1227	357	1225:1227	PEY vs. egg-yolk comparative metabolomic analyses showed significative differences in the concentrations of at least 140 molecules, and in 357 in the lipidomic analyses, demonstrating the complexity of PEY.
32899660	0	49	theme	Related	38:44	arg1	Mechanisms					46:55	Related Mechanisms	38:55	Related Mechanisms of Processed Egg Yolk	38:77	In Vivo Anti-Inflammatory Effects and Related Mechanisms of Processed Egg Yolk, a Potential Anti-Inflammaging Dietary Supplement.
32899660	4	50	theme	samples	557:563	arg1	properties					529:538	ex vivo properties	521:538	ex vivo properties of derived serum samples	521:563	Cell culture was used to explore ex vivo properties of derived serum samples.
32899660	8	51	theme	growth	963:968	arg1	factor					970:975	insulin-like growth factor 1	950:977	insulin-like growth factor 1 (IGF-1)	950:985	Immunochemical analyses demonstrated increased concentrations of insulin-like growth factor 1 (IGF-1), connective tissue growth factor (CTGF), and platelet-derived growth factor (PDGF) in the extract.
32899660	8	51	theme	growth	963:968	arg1	IGF-1					980:984	IGF-1	980:984	IGF-1	980:984	Immunochemical analyses demonstrated increased concentrations of insulin-like growth factor 1 (IGF-1), connective tissue growth factor (CTGF), and platelet-derived growth factor (PDGF) in the extract.
32899660	3	52	dep	in	413:414	arg1	vivo					416:419	vivo	416:419	vivo	416:419	These were evaluated in a lipopolysaccharide (LPS)-challenge in 1-month male rats by in vivo circulating cytokine profiles measured by multiplexing techniques.
32899660	9	53	theme	metabolomic	1115:1125	arg1	analyses					1127:1134	PEY vs. egg-yolk comparative metabolomic analyses	1086:1134	PEY vs. egg-yolk comparative metabolomic analyses	1086:1134	PEY vs. egg-yolk comparative metabolomic analyses showed significative differences in the concentrations of at least 140 molecules, and in 357 in the lipidomic analyses, demonstrating the complexity of PEY.
32899660	2	54	contain	has	288:290	arg2	properties					316:325	inflammation modulatory properties	292:325	inflammation modulatory properties	292:325	We have developed a novel processed egg-yolk (PEY) complement, and we have tested whether it has inflammation modulatory properties.
32899660	2	54	contain	has	288:290	arg1	it					285:286	it	285:286	it	285:286	We have developed a novel processed egg-yolk (PEY) complement, and we have tested whether it has inflammation modulatory properties.
32899660	9	55	theme	lipidomic	1236:1244	arg1	analyses					1246:1253	the lipidomic analyses	1232:1253	the lipidomic analyses	1232:1253	PEY vs. egg-yolk comparative metabolomic analyses showed significative differences in the concentrations of at least 140 molecules, and in 357 in the lipidomic analyses, demonstrating the complexity of PEY.
32899660	2	56	theme	PEY	241:243	arg1	complement					246:255	a novel processed egg-yolk (PEY) complement	213:255	a novel processed egg-yolk (PEY) complement	213:255	We have developed a novel processed egg-yolk (PEY) complement, and we have tested whether it has inflammation modulatory properties.
32899660	7	57	dep	Raw	848:850	arg1	line					879:882	macrophage-like cell line	858:882	the Raw 264.7 macrophage-like cell line	844:882	Further, serum from PEY-treated animals abrogated LPS-induced iNOS build-up of the Raw 264.7 macrophage-like cell line.
32899660	9	58	theme	molecules	1207:1215	arg1	concentrations					1176:1189	the concentrations	1172:1189	the concentrations of at least 140 molecules	1172:1215	PEY vs. egg-yolk comparative metabolomic analyses showed significative differences in the concentrations of at least 140 molecules, and in 357 in the lipidomic analyses, demonstrating the complexity of PEY.
32899660	3	59	theme	1-month	392:398	arg1	rats					405:408	1-month male rats	392:408	1-month male rats	392:408	These were evaluated in a lipopolysaccharide (LPS)-challenge in 1-month male rats by in vivo circulating cytokine profiles measured by multiplexing techniques.
32899660	3	60	theme	lipopolysaccharide	354:371	arg1	-challenge					378:387	a lipopolysaccharide (LPS)-challenge	352:387	a lipopolysaccharide (LPS)-challenge in 1-month male rats	352:408	These were evaluated in a lipopolysaccharide (LPS)-challenge in 1-month male rats by in vivo circulating cytokine profiles measured by multiplexing techniques.
32899660	7	61	theme	LPS-induced	815:825	arg1	build-up					832:839	LPS-induced iNOS build-up	815:839	LPS-induced iNOS build-up of the Raw 264.7 macrophage-like cell line	815:882	Further, serum from PEY-treated animals abrogated LPS-induced iNOS build-up of the Raw 264.7 macrophage-like cell line.
32899660	8	62	theme	insulin-like	950:961	arg1	factor					970:975	insulin-like growth factor 1	950:977	insulin-like growth factor 1 (IGF-1)	950:985	Immunochemical analyses demonstrated increased concentrations of insulin-like growth factor 1 (IGF-1), connective tissue growth factor (CTGF), and platelet-derived growth factor (PDGF) in the extract.
32899660	8	62	theme	insulin-like	950:961	arg1	IGF-1					980:984	IGF-1	980:984	IGF-1	980:984	Immunochemical analyses demonstrated increased concentrations of insulin-like growth factor 1 (IGF-1), connective tissue growth factor (CTGF), and platelet-derived growth factor (PDGF) in the extract.
32899660	3	63	theme	male	400:403	arg1	rats					405:408	1-month male rats	392:408	1-month male rats	392:408	These were evaluated in a lipopolysaccharide (LPS)-challenge in 1-month male rats by in vivo circulating cytokine profiles measured by multiplexing techniques.
32899660	2	64	theme	processed	221:229	arg1	complement					246:255	a novel processed egg-yolk (PEY) complement	213:255	a novel processed egg-yolk (PEY) complement	213:255	We have developed a novel processed egg-yolk (PEY) complement, and we have tested whether it has inflammation modulatory properties.
32899660	2	65	theme	egg-yolk	231:238	arg1	complement					246:255	a novel processed egg-yolk (PEY) complement	213:255	a novel processed egg-yolk (PEY) complement	213:255	We have developed a novel processed egg-yolk (PEY) complement, and we have tested whether it has inflammation modulatory properties.
32899660	9	66	theme	significative	1143:1155	arg1	differences					1157:1167	significative differences	1143:1167	significative differences in the concentrations of at least 140 molecules, and in 357 in the lipidomic analyses	1143:1253	PEY vs. egg-yolk comparative metabolomic analyses showed significative differences in the concentrations of at least 140 molecules, and in 357 in the lipidomic analyses, demonstrating the complexity of PEY.
32899660	9	67	from	357	1225:1227	arg1	analyses					1246:1253	the lipidomic analyses	1232:1253	the lipidomic analyses	1232:1253	PEY vs. egg-yolk comparative metabolomic analyses showed significative differences in the concentrations of at least 140 molecules, and in 357 in the lipidomic analyses, demonstrating the complexity of PEY.
32899660	10	68	theme	immunomodulatory	1338:1353	arg1	PEY					1303:1305	PEY	1303:1305	PEY	1303:1305	Globally, PEY acts as an orally-bioavailable immunomodulatory extract that may be of interest in those conditions associated with disarranged inflammation, such as inflammaging.
32899660	10	68	theme	immunomodulatory	1338:1353	arg1	extract					1355:1361	an orally-bioavailable immunomodulatory extract	1315:1361	an orally-bioavailable immunomodulatory extract that may be of interest in those conditions associated with disarranged inflammation, such as inflammaging	1315:1468	Globally, PEY acts as an orally-bioavailable immunomodulatory extract that may be of interest in those conditions associated with disarranged inflammation, such as inflammaging.
32899660	2	69	theme	novel	215:219	arg1	complement					246:255	a novel processed egg-yolk (PEY) complement	213:255	a novel processed egg-yolk (PEY) complement	213:255	We have developed a novel processed egg-yolk (PEY) complement, and we have tested whether it has inflammation modulatory properties.
32899660	8	70	theme	factor	970:975	arg1	concentrations					932:945	increased concentrations	922:945	increased concentrations of insulin-like growth factor 1 (IGF-1), connective tissue growth factor (CTGF), and platelet-derived growth factor (PDGF) in the extract	922:1083	Immunochemical analyses demonstrated increased concentrations of insulin-like growth factor 1 (IGF-1), connective tissue growth factor (CTGF), and platelet-derived growth factor (PDGF) in the extract.
32899660	1	71	theme	Egg-yolk	130:137	arg1	supplements					145:155	Egg-yolk based supplements	130:155	Egg-yolk based supplements	130:155	Egg-yolk based supplements have demonstrated biological effects.
32899660	4	72	used	used	505:508	arg2	culture					493:499	Cell culture	488:499	Cell culture	488:499	Cell culture was used to explore ex vivo properties of derived serum samples.
32899660	1	73	theme	based	139:143	arg1	supplements					145:155	Egg-yolk based supplements	130:155	Egg-yolk based supplements	130:155	Egg-yolk based supplements have demonstrated biological effects.
32899660	8	74	link	platelet-derived	1032:1047	arg1	PDGF					1064:1067	PDGF	1064:1067	PDGF	1064:1067	Immunochemical analyses demonstrated increased concentrations of insulin-like growth factor 1 (IGF-1), connective tissue growth factor (CTGF), and platelet-derived growth factor (PDGF) in the extract.
32899660	8	74	link	platelet-derived	1032:1047	arg1	factor					1056:1061	platelet-derived growth factor	1032:1061	platelet-derived growth factor (PDGF)	1032:1068	Immunochemical analyses demonstrated increased concentrations of insulin-like growth factor 1 (IGF-1), connective tissue growth factor (CTGF), and platelet-derived growth factor (PDGF) in the extract.
32899660	7	75	theme	Raw	848:850	arg1	build-up					832:839	LPS-induced iNOS build-up	815:839	LPS-induced iNOS build-up of the Raw 264.7 macrophage-like cell line	815:882	Further, serum from PEY-treated animals abrogated LPS-induced iNOS build-up of the Raw 264.7 macrophage-like cell line.
32899660	0	76	theme	Egg	70:72	arg1	Yolk					74:77	Processed Egg Yolk	60:77	Processed Egg Yolk	60:77	In Vivo Anti-Inflammatory Effects and Related Mechanisms of Processed Egg Yolk, a Potential Anti-Inflammaging Dietary Supplement.
32899660	8	77	theme	growth	1006:1011	arg1	CTGF					1021:1024	CTGF	1021:1024	CTGF	1021:1024	Immunochemical analyses demonstrated increased concentrations of insulin-like growth factor 1 (IGF-1), connective tissue growth factor (CTGF), and platelet-derived growth factor (PDGF) in the extract.
32899660	8	77	theme	growth	1006:1011	arg1	factor					1013:1018	connective tissue growth factor	988:1018	connective tissue growth factor (CTGF)	988:1025	Immunochemical analyses demonstrated increased concentrations of insulin-like growth factor 1 (IGF-1), connective tissue growth factor (CTGF), and platelet-derived growth factor (PDGF) in the extract.
32899660	0	78	theme	Processed	60:68	arg1	Yolk					74:77	Processed Egg Yolk	60:77	Processed Egg Yolk	60:77	In Vivo Anti-Inflammatory Effects and Related Mechanisms of Processed Egg Yolk, a Potential Anti-Inflammaging Dietary Supplement.
32899660	0	79	dep	In	0:1	arg1	Vivo					3:6	Vivo	3:6	Vivo	3:6	In Vivo Anti-Inflammatory Effects and Related Mechanisms of Processed Egg Yolk, a Potential Anti-Inflammaging Dietary Supplement.
32899660	4	80	theme	ex	521:522	arg1	properties					529:538	ex vivo properties	521:538	ex vivo properties of derived serum samples	521:563	Cell culture was used to explore ex vivo properties of derived serum samples.
34218911	6	0	theme	apparent	888:895	arg1	effect					897:902	no apparent effect	885:902	no apparent effect of lactose feeding on the α-diversity and overall composition of rumen microbiota	885:984	There was no apparent effect of lactose feeding on the α-diversity and overall composition of rumen microbiota.
34218911	3	1	from	%	589:589	arg1	basis					629:633	a dry matter basis	616:633	a dry matter basis	616:633	Thirty Holstein bull calves were divided into 2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis.
34218911	8	2	theme	LAC10	1473:1477	arg1	groups					1479:1484	the control and LAC10 groups	1457:1484	groups	1479:1484	Conversely, the relative abundance of Mitsuokella spp., which produce lactate, succinate, and acetate, was significantly higher in the rumen of calves that were fed lactose, whereas the lactate concentration did not differ between the control and LAC10 groups.
34218911	4	3	theme	kleingrass	721:730	arg1	hay					732:734	kleingrass hay	721:734	kleingrass hay from d 35	721:744	All calves were fed their respective treatment calf starter ad libitum from d 7, and kleingrass hay from d 35.
34218911	2	4	from	shift	284:288	arg1	microbiota					299:308	rumen microbiota	293:308	rumen microbiota	293:308	We conducted the present study as a follow-up study to identify the shift in rumen microbiota and its relation to rumen fermentation when calves are fed a lactose-containing starter.
34218911	5	5	used	used	815:818	arg2	digesta					753:759	Rumen digesta	747:759	Rumen digesta	747:759	Rumen digesta were collected on d 80 (i.e., 3 wk after weaning) and used to analyze rumen microbiota and fermentation products.
34218911	3	6	dep	%	589:589	arg1	=					607:607	=	607:607	=	607:607	Thirty Holstein bull calves were divided into 2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis.
34218911	6	7	theme	rumen	969:973	arg1	microbiota					975:984	rumen microbiota	969:984	rumen microbiota	969:984	There was no apparent effect of lactose feeding on the α-diversity and overall composition of rumen microbiota.
34218911	7	8	theme	LAC10	1212:1216	arg1	groups					1218:1223	the control and LAC10 groups	1196:1223	groups	1218:1223	Amplicon sequencing and real-time PCR quantification of the 16S rRNA gene confirmed that the abundance of butyrate-producing bacteria (i.e., Butyrivibrio group and Megasphaera elsdenii) did not differ between the control and LAC10 groups.
34218911	2	9	from	relation	318:325	arg1	microbiota					299:308	rumen microbiota	293:308	rumen microbiota	293:308	We conducted the present study as a follow-up study to identify the shift in rumen microbiota and its relation to rumen fermentation when calves are fed a lactose-containing starter.
34218911	7	10	theme	control	1200:1206	arg1	groups					1218:1223	the control and LAC10 groups	1196:1223	groups	1218:1223	Amplicon sequencing and real-time PCR quantification of the 16S rRNA gene confirmed that the abundance of butyrate-producing bacteria (i.e., Butyrivibrio group and Megasphaera elsdenii) did not differ between the control and LAC10 groups.
34218911	3	11	dep	groups	470:475	arg1	starter					534:540	calf starter	529:540	calf starter	529:540	Thirty Holstein bull calves were divided into 2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis.
34218911	3	11	dep	groups	470:475	arg1	starter					494:500	texturized calf starter	478:500	texturized calf starter (i.e., control; n = 15)	478:524	Thirty Holstein bull calves were divided into 2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis.
34218911	3	11	dep	groups	470:475	arg1	groups					470:475	2 calf starter treatment groups	445:475	2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis	445:633	Thirty Holstein bull calves were divided into 2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis.
34218911	11	12	theme	LAC10	1812:1816	arg1	group					1818:1822	the LAC10 group	1808:1822	the LAC10 group	1808:1822	In addition, we observed a higher abundance of Coriobacteriaceae and Pseudoramibacter-Eubacterium in the LAC10 group.
34218911	13	13	theme	lactose-fed	2139:2149	arg1	calves					2151:2156	lactose-fed calves	2139:2156	lactose-fed calves	2139:2156	Therefore, the higher abundance of Coriobacteriaceae, Mitsuokella spp., and Pseudoramibacter-Eubacterium in the rumen of lactose-fed calves partially explains the increase in the proportion of rumen acetate that was observed in our previous study.
34218911	5	14	theme	Rumen	747:751	arg1	digesta					753:759	Rumen digesta	747:759	Rumen digesta	747:759	Rumen digesta were collected on d 80 (i.e., 3 wk after weaning) and used to analyze rumen microbiota and fermentation products.
34218911	8	15	theme	relative	1242:1249	arg1	abundance					1251:1259	the relative abundance	1238:1259	the relative abundance of Mitsuokella spp., which produce lactate, succinate, and acetate,	1238:1327	Conversely, the relative abundance of Mitsuokella spp., which produce lactate, succinate, and acetate, was significantly higher in the rumen of calves that were fed lactose, whereas the lactate concentration did not differ between the control and LAC10 groups.
34218911	8	15	theme	relative	1242:1249	arg1	higher					1347:1352	higher	1347:1352	higher	1347:1352	Conversely, the relative abundance of Mitsuokella spp., which produce lactate, succinate, and acetate, was significantly higher in the rumen of calves that were fed lactose, whereas the lactate concentration did not differ between the control and LAC10 groups.
34218911	5	16	dep	wk	793:794	arg1	i.e.					785:788	i.e.	785:788	i.e.	785:788	Rumen digesta were collected on d 80 (i.e., 3 wk after weaning) and used to analyze rumen microbiota and fermentation products.
34218911	1	17	theme	gut	189:191	arg1	development					193:203	gut development	189:203	gut development	189:203	In our previous studies, we revealed the effect of lactose inclusion in calf starters on the growth performance and gut development of calves.
34218911	12	18	theme	acetate-producing	1859:1875	arg1	bacteria					1877:1884	acetate-producing bacteria	1859:1884	acetate-producing bacteria	1859:1884	Both these bacterial taxa include acetate-producing bacteria, and a positive correlation between the acetate-to-propionate ratio and the abundance of Pseudoramibacter-Eubacterium was observed.
34218911	3	19	theme	matter	622:627	arg1	basis					629:633	a dry matter basis	616:633	a dry matter basis	616:633	Thirty Holstein bull calves were divided into 2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis.
34218911	5	20	theme	rumen	831:835	arg1	microbiota					837:846	rumen microbiota	831:846	rumen microbiota	831:846	Rumen digesta were collected on d 80 (i.e., 3 wk after weaning) and used to analyze rumen microbiota and fermentation products.
34218911	3	21	dep	starter	494:500	arg1	=					520:520	=	520:520	=	520:520	Thirty Holstein bull calves were divided into 2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis.
34218911	11	22	theme	Pseudoramibacter-Eubacterium	1776:1803	arg1	abundance					1741:1749	a higher abundance	1732:1749	a higher abundance of Coriobacteriaceae and Pseudoramibacter-Eubacterium in the LAC10 group	1732:1822	In addition, we observed a higher abundance of Coriobacteriaceae and Pseudoramibacter-Eubacterium in the LAC10 group.
34218911	7	23	theme	Megasphaera	1151:1161	arg1	elsdenii					1163:1170	Megasphaera elsdenii	1151:1170	Megasphaera elsdenii	1151:1170	Amplicon sequencing and real-time PCR quantification of the 16S rRNA gene confirmed that the abundance of butyrate-producing bacteria (i.e., Butyrivibrio group and Megasphaera elsdenii) did not differ between the control and LAC10 groups.
34218911	0	24	theme	lactose-fed	53:63	arg1	calves					65:70	lactose-fed calves	53:70	lactose-fed calves	53:70	Rumen microbiota and its relation to fermentation in lactose-fed calves.
34218911	11	25	theme	higher	1734:1739	arg1	abundance					1741:1749	a higher abundance	1732:1749	a higher abundance of Coriobacteriaceae and Pseudoramibacter-Eubacterium in the LAC10 group	1732:1822	In addition, we observed a higher abundance of Coriobacteriaceae and Pseudoramibacter-Eubacterium in the LAC10 group.
34218911	1	26	theme	lactose	124:130	arg1	inclusion					132:140	lactose inclusion	124:140	lactose inclusion	124:140	In our previous studies, we revealed the effect of lactose inclusion in calf starters on the growth performance and gut development of calves.
34218911	7	27	theme	real-time	1011:1019	arg1	PCR					1021:1023	real-time PCR	1011:1023	real-time PCR quantification	1011:1038	Amplicon sequencing and real-time PCR quantification of the 16S rRNA gene confirmed that the abundance of butyrate-producing bacteria (i.e., Butyrivibrio group and Megasphaera elsdenii) did not differ between the control and LAC10 groups.
34218911	9	28	theme	Mitsuokella	1572:1582	arg1	spp					1584:1586	Mitsuokella spp	1572:1586	Mitsuokella spp	1572:1586	These findings suggest that the lactate production can be elevated by an increase of Mitsuokella spp.
34218911	0	29	from	relation	25:32	arg1	calves					65:70	lactose-fed calves	53:70	lactose-fed calves	53:70	Rumen microbiota and its relation to fermentation in lactose-fed calves.
34218911	13	30	from	abundance	2040:2048	arg1	rumen					2130:2134	the rumen	2126:2134	the rumen of lactose-fed calves	2126:2156	Therefore, the higher abundance of Coriobacteriaceae, Mitsuokella spp., and Pseudoramibacter-Eubacterium in the rumen of lactose-fed calves partially explains the increase in the proportion of rumen acetate that was observed in our previous study.
34218911	0	31	theme	Rumen	0:4	arg1	microbiota					6:15	Rumen microbiota	0:15	Rumen microbiota	0:15	Rumen microbiota and its relation to fermentation in lactose-fed calves.
34218911	1	32	theme	previous	80:87	arg1	studies					89:95	our previous studies	76:95	our previous studies	76:95	In our previous studies, we revealed the effect of lactose inclusion in calf starters on the growth performance and gut development of calves.
34218911	7	33	theme	Butyrivibrio	1128:1139	arg1	group					1141:1145	Butyrivibrio group	1128:1145	Butyrivibrio group	1128:1145	Amplicon sequencing and real-time PCR quantification of the 16S rRNA gene confirmed that the abundance of butyrate-producing bacteria (i.e., Butyrivibrio group and Megasphaera elsdenii) did not differ between the control and LAC10 groups.
34218911	13	34	dep	Mitsuokella	2072:2082	arg1	spp.					2084:2087	Mitsuokella spp.	2072:2087	Mitsuokella spp.	2072:2087	Therefore, the higher abundance of Coriobacteriaceae, Mitsuokella spp., and Pseudoramibacter-Eubacterium in the rumen of lactose-fed calves partially explains the increase in the proportion of rumen acetate that was observed in our previous study.
34218911	2	35	theme	lactose-containing	371:388	arg1	starter					390:396	a lactose-containing starter	369:396	a lactose-containing starter	369:396	We conducted the present study as a follow-up study to identify the shift in rumen microbiota and its relation to rumen fermentation when calves are fed a lactose-containing starter.
34218911	13	36	theme	Coriobacteriaceae	2053:2069	arg1	abundance					2040:2048	the higher abundance	2029:2048	the higher abundance of Coriobacteriaceae, Mitsuokella spp., and Pseudoramibacter-Eubacterium in the rumen of lactose-fed calves	2029:2156	Therefore, the higher abundance of Coriobacteriaceae, Mitsuokella spp., and Pseudoramibacter-Eubacterium in the rumen of lactose-fed calves partially explains the increase in the proportion of rumen acetate that was observed in our previous study.
34218911	11	37	theme	Coriobacteriaceae	1754:1770	arg1	abundance					1741:1749	a higher abundance	1732:1749	a higher abundance of Coriobacteriaceae and Pseudoramibacter-Eubacterium in the LAC10 group	1732:1822	In addition, we observed a higher abundance of Coriobacteriaceae and Pseudoramibacter-Eubacterium in the LAC10 group.
34218911	4	38	theme	treatment	673:681	arg1	libitum					699:705	their respective treatment calf starter ad libitum	656:705	their respective treatment calf starter ad libitum	656:705	All calves were fed their respective treatment calf starter ad libitum from d 7, and kleingrass hay from d 35.
34218911	3	39	theme	bull	415:418	arg1	calves					420:425	Thirty Holstein bull calves	399:425	Thirty Holstein bull calves	399:425	Thirty Holstein bull calves were divided into 2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis.
34218911	3	40	dep	=	520:520	arg1	control					509:515	control	509:515	control	509:515	Thirty Holstein bull calves were divided into 2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis.
34218911	3	40	dep	=	520:520	arg1	i.e.					503:506	i.e.	503:506	i.e.	503:506	Thirty Holstein bull calves were divided into 2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis.
34218911	8	41	dep	Mitsuokella	1264:1274	arg1	spp.					1276:1279	Mitsuokella spp., which produce lactate, succinate, and acetate,	1264:1327	spp.	1276:1279	Conversely, the relative abundance of Mitsuokella spp., which produce lactate, succinate, and acetate, was significantly higher in the rumen of calves that were fed lactose, whereas the lactate concentration did not differ between the control and LAC10 groups.
34218911	8	41	dep	Mitsuokella	1264:1274	arg1	produce					1288:1294	produce	1288:1294	produce lactate, succinate, and acetate	1288:1326	Conversely, the relative abundance of Mitsuokella spp., which produce lactate, succinate, and acetate, was significantly higher in the rumen of calves that were fed lactose, whereas the lactate concentration did not differ between the control and LAC10 groups.
34218911	7	42	theme	bacteria	1112:1119	arg1	abundance					1080:1088	the abundance	1076:1088	the abundance of butyrate-producing bacteria (i.e., Butyrivibrio group and Megasphaera elsdenii)	1076:1171	Amplicon sequencing and real-time PCR quantification of the 16S rRNA gene confirmed that the abundance of butyrate-producing bacteria (i.e., Butyrivibrio group and Megasphaera elsdenii) did not differ between the control and LAC10 groups.
34218911	4	43	theme	starter	688:694	arg1	libitum					699:705	their respective treatment calf starter ad libitum	656:705	their respective treatment calf starter ad libitum	656:705	All calves were fed their respective treatment calf starter ad libitum from d 7, and kleingrass hay from d 35.
34218911	3	44	theme	calf	447:450	arg1	groups					470:475	2 calf starter treatment groups	445:475	2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis	445:633	Thirty Holstein bull calves were divided into 2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis.
34218911	3	44	theme	calf	447:450	arg1	starter					494:500	texturized calf starter	478:500	texturized calf starter (i.e., control; n = 15)	478:524	Thirty Holstein bull calves were divided into 2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis.
34218911	3	44	theme	calf	447:450	arg1	starter					534:540	calf starter	529:540	calf starter	529:540	Thirty Holstein bull calves were divided into 2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis.
34218911	3	45	dep	=	607:607	arg1	LAC10					598:602	LAC10	598:602	LAC10	598:602	Thirty Holstein bull calves were divided into 2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis.
34218911	3	45	dep	=	607:607	arg1	i.e.					592:595	i.e.	592:595	i.e.	592:595	Thirty Holstein bull calves were divided into 2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis.
34218911	8	46	theme	calves	1370:1375	arg1	rumen					1361:1365	the rumen	1357:1365	the rumen of calves that were fed lactose	1357:1397	Conversely, the relative abundance of Mitsuokella spp., which produce lactate, succinate, and acetate, was significantly higher in the rumen of calves that were fed lactose, whereas the lactate concentration did not differ between the control and LAC10 groups.
34218911	12	47	theme	Pseudoramibacter-Eubacterium	1975:2002	arg1	abundance					1962:1970	the abundance	1958:1970	the abundance of Pseudoramibacter-Eubacterium	1958:2002	Both these bacterial taxa include acetate-producing bacteria, and a positive correlation between the acetate-to-propionate ratio and the abundance of Pseudoramibacter-Eubacterium was observed.
34218911	12	47	theme	Pseudoramibacter-Eubacterium	1975:2002	arg1	ratio					1948:1952	the acetate-to-propionate ratio	1922:1952	the acetate-to-propionate ratio	1922:1952	Both these bacterial taxa include acetate-producing bacteria, and a positive correlation between the acetate-to-propionate ratio and the abundance of Pseudoramibacter-Eubacterium was observed.
34218911	6	48	theme	lactose	907:913	arg1	feeding					915:921	lactose feeding	907:921	lactose feeding	907:921	There was no apparent effect of lactose feeding on the α-diversity and overall composition of rumen microbiota.
34218911	1	49	theme	calves	208:213	arg1	performance					173:183	growth performance	166:183	growth performance	166:183	In our previous studies, we revealed the effect of lactose inclusion in calf starters on the growth performance and gut development of calves.
34218911	1	49	theme	calves	208:213	arg1	development					193:203	gut development	189:203	gut development	189:203	In our previous studies, we revealed the effect of lactose inclusion in calf starters on the growth performance and gut development of calves.
34218911	13	50	theme	rumen	2211:2215	arg1	acetate					2217:2223	rumen acetate	2211:2223	rumen acetate	2211:2223	Therefore, the higher abundance of Coriobacteriaceae, Mitsuokella spp., and Pseudoramibacter-Eubacterium in the rumen of lactose-fed calves partially explains the increase in the proportion of rumen acetate that was observed in our previous study.
34218911	6	51	from	effect	897:902	arg1	composition					954:964	the α-diversity and overall composition	926:964	the α-diversity and overall composition of rumen microbiota	926:984	There was no apparent effect of lactose feeding on the α-diversity and overall composition of rumen microbiota.
34218911	3	52	theme	treatment	460:468	arg1	groups					470:475	2 calf starter treatment groups	445:475	2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis	445:633	Thirty Holstein bull calves were divided into 2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis.
34218911	3	52	theme	treatment	460:468	arg1	starter					494:500	texturized calf starter	478:500	texturized calf starter (i.e., control; n = 15)	478:524	Thirty Holstein bull calves were divided into 2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis.
34218911	3	52	theme	treatment	460:468	arg1	starter					534:540	calf starter	529:540	calf starter	529:540	Thirty Holstein bull calves were divided into 2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis.
34218911	7	53	theme	rRNA	1051:1054	arg1	gene					1056:1059	the 16S rRNA gene	1043:1059	the 16S rRNA gene	1043:1059	Amplicon sequencing and real-time PCR quantification of the 16S rRNA gene confirmed that the abundance of butyrate-producing bacteria (i.e., Butyrivibrio group and Megasphaera elsdenii) did not differ between the control and LAC10 groups.
34218911	10	54	theme	propionate	1663:1672	arg1	lower					1678:1682	lower	1678:1682	lower	1678:1682	and then converted into butyrate, not propionate, since the proportion of propionate was lower in lactose-fed calves.
34218911	10	54	theme	propionate	1663:1672	arg1	proportion					1649:1658	the proportion	1645:1658	the proportion of propionate	1645:1672	and then converted into butyrate, not propionate, since the proportion of propionate was lower in lactose-fed calves.
34218911	2	55	theme	follow-up	252:260	arg1	study					262:266	a follow-up study	250:266	a follow-up study	250:266	We conducted the present study as a follow-up study to identify the shift in rumen microbiota and its relation to rumen fermentation when calves are fed a lactose-containing starter.
34218911	6	56	theme	microbiota	975:984	arg1	composition					954:964	the α-diversity and overall composition	926:964	the α-diversity and overall composition of rumen microbiota	926:984	There was no apparent effect of lactose feeding on the α-diversity and overall composition of rumen microbiota.
34218911	8	57	theme	lactate	1412:1418	arg1	concentration					1420:1432	the lactate concentration	1408:1432	the lactate concentration	1408:1432	Conversely, the relative abundance of Mitsuokella spp., which produce lactate, succinate, and acetate, was significantly higher in the rumen of calves that were fed lactose, whereas the lactate concentration did not differ between the control and LAC10 groups.
34218911	3	58	theme	calf	489:492	arg1	groups					470:475	2 calf starter treatment groups	445:475	2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis	445:633	Thirty Holstein bull calves were divided into 2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis.
34218911	3	58	theme	calf	489:492	arg1	starter					494:500	texturized calf starter	478:500	texturized calf starter (i.e., control; n = 15)	478:524	Thirty Holstein bull calves were divided into 2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis.
34218911	1	59	theme	calf	145:148	arg1	starters					150:157	calf starters	145:157	calf starters	145:157	In our previous studies, we revealed the effect of lactose inclusion in calf starters on the growth performance and gut development of calves.
34218911	4	60	theme	ad	696:697	arg1	libitum					699:705	their respective treatment calf starter ad libitum	656:705	their respective treatment calf starter ad libitum	656:705	All calves were fed their respective treatment calf starter ad libitum from d 7, and kleingrass hay from d 35.
34218911	11	61	from	abundance	1741:1749	arg1	group					1818:1822	the LAC10 group	1808:1822	the LAC10 group	1808:1822	In addition, we observed a higher abundance of Coriobacteriaceae and Pseudoramibacter-Eubacterium in the LAC10 group.
34218911	10	62	theme	lactose-fed	1687:1697	arg1	calves					1699:1704	lactose-fed calves	1687:1704	lactose-fed calves	1687:1704	and then converted into butyrate, not propionate, since the proportion of propionate was lower in lactose-fed calves.
34218911	6	63	theme	overall	946:952	arg1	composition					954:964	the α-diversity and overall composition	926:964	the α-diversity and overall composition of rumen microbiota	926:984	There was no apparent effect of lactose feeding on the α-diversity and overall composition of rumen microbiota.
34218911	12	64	theme	acetate-to-propionate	1926:1946	arg1	ratio					1948:1952	the acetate-to-propionate ratio	1922:1952	the acetate-to-propionate ratio	1922:1952	Both these bacterial taxa include acetate-producing bacteria, and a positive correlation between the acetate-to-propionate ratio and the abundance of Pseudoramibacter-Eubacterium was observed.
34218911	3	65	theme	dry	618:620	arg1	basis					629:633	a dry matter basis	616:633	a dry matter basis	616:633	Thirty Holstein bull calves were divided into 2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis.
34218911	1	66	theme	growth	166:171	arg1	performance					173:183	growth performance	166:183	growth performance	166:183	In our previous studies, we revealed the effect of lactose inclusion in calf starters on the growth performance and gut development of calves.
34218911	6	67	theme	α-diversity	930:940	arg1	composition					954:964	the α-diversity and overall composition	926:964	the α-diversity and overall composition of rumen microbiota	926:984	There was no apparent effect of lactose feeding on the α-diversity and overall composition of rumen microbiota.
34218911	2	68	theme	rumen	293:297	arg1	microbiota					299:308	rumen microbiota	293:308	rumen microbiota	293:308	We conducted the present study as a follow-up study to identify the shift in rumen microbiota and its relation to rumen fermentation when calves are fed a lactose-containing starter.
34218911	13	69	theme	calves	2151:2156	arg1	rumen					2130:2134	the rumen	2126:2134	the rumen of lactose-fed calves	2126:2156	Therefore, the higher abundance of Coriobacteriaceae, Mitsuokella spp., and Pseudoramibacter-Eubacterium in the rumen of lactose-fed calves partially explains the increase in the proportion of rumen acetate that was observed in our previous study.
34218911	8	70	theme	Mitsuokella	1264:1274	arg1	abundance					1251:1259	the relative abundance	1238:1259	the relative abundance of Mitsuokella spp., which produce lactate, succinate, and acetate,	1238:1327	Conversely, the relative abundance of Mitsuokella spp., which produce lactate, succinate, and acetate, was significantly higher in the rumen of calves that were fed lactose, whereas the lactate concentration did not differ between the control and LAC10 groups.
34218911	8	70	theme	Mitsuokella	1264:1274	arg1	higher					1347:1352	higher	1347:1352	higher	1347:1352	Conversely, the relative abundance of Mitsuokella spp., which produce lactate, succinate, and acetate, was significantly higher in the rumen of calves that were fed lactose, whereas the lactate concentration did not differ between the control and LAC10 groups.
34218911	12	71	theme	positive	1893:1900	arg1	correlation					1902:1912	a positive correlation	1891:1912	a positive correlation between the acetate-to-propionate ratio and the abundance of Pseudoramibacter-Eubacterium	1891:2002	Both these bacterial taxa include acetate-producing bacteria, and a positive correlation between the acetate-to-propionate ratio and the abundance of Pseudoramibacter-Eubacterium was observed.
34218911	13	72	theme	Pseudoramibacter-Eubacterium	2094:2121	arg1	abundance					2040:2048	the higher abundance	2029:2048	the higher abundance of Coriobacteriaceae, Mitsuokella spp., and Pseudoramibacter-Eubacterium in the rumen of lactose-fed calves	2029:2156	Therefore, the higher abundance of Coriobacteriaceae, Mitsuokella spp., and Pseudoramibacter-Eubacterium in the rumen of lactose-fed calves partially explains the increase in the proportion of rumen acetate that was observed in our previous study.
34218911	4	73	from	d	741:741	arg1	hay					732:734	kleingrass hay	721:734	kleingrass hay from d 35	721:744	All calves were fed their respective treatment calf starter ad libitum from d 7, and kleingrass hay from d 35.
34218911	4	73	from	d	741:741	arg1	d					712:712	d 7	712:714	d 7	712:714	All calves were fed their respective treatment calf starter ad libitum from d 7, and kleingrass hay from d 35.
34218911	2	74	theme	present	233:239	arg1	study					241:245	the present study	229:245	the present study as a follow-up study	229:266	We conducted the present study as a follow-up study to identify the shift in rumen microbiota and its relation to rumen fermentation when calves are fed a lactose-containing starter.
34218911	9	75	theme	lactate	1519:1525	arg1	production					1527:1536	the lactate production	1515:1536	the lactate production	1515:1536	These findings suggest that the lactate production can be elevated by an increase of Mitsuokella spp.
34218911	5	76	theme	fermentation	852:863	arg1	products					865:872	fermentation products	852:872	fermentation products	852:872	Rumen digesta were collected on d 80 (i.e., 3 wk after weaning) and used to analyze rumen microbiota and fermentation products.
34218911	3	77	theme	calf	529:532	arg1	starter					534:540	calf starter	529:540	calf starter	529:540	Thirty Holstein bull calves were divided into 2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis.
34218911	3	77	theme	calf	529:532	arg1	groups					470:475	2 calf starter treatment groups	445:475	2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis	445:633	Thirty Holstein bull calves were divided into 2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis.
34218911	13	78	theme	Mitsuokella	2072:2082	arg1	abundance					2040:2048	the higher abundance	2029:2048	the higher abundance of Coriobacteriaceae, Mitsuokella spp., and Pseudoramibacter-Eubacterium in the rumen of lactose-fed calves	2029:2156	Therefore, the higher abundance of Coriobacteriaceae, Mitsuokella spp., and Pseudoramibacter-Eubacterium in the rumen of lactose-fed calves partially explains the increase in the proportion of rumen acetate that was observed in our previous study.
34218911	7	79	theme	PCR	1021:1023	arg1	quantification					1025:1038	real-time PCR quantification	1011:1038	real-time PCR quantification	1011:1038	Amplicon sequencing and real-time PCR quantification of the 16S rRNA gene confirmed that the abundance of butyrate-producing bacteria (i.e., Butyrivibrio group and Megasphaera elsdenii) did not differ between the control and LAC10 groups.
34218911	1	80	theme	inclusion	132:140	arg1	effect					114:119	the effect	110:119	the effect of lactose inclusion in calf starters on the growth performance and gut development of calves	110:213	In our previous studies, we revealed the effect of lactose inclusion in calf starters on the growth performance and gut development of calves.
34218911	0	81	from	microbiota	6:15	arg1	calves					65:70	lactose-fed calves	53:70	lactose-fed calves	53:70	Rumen microbiota and its relation to fermentation in lactose-fed calves.
34218911	12	82	theme	bacterial	1836:1844	arg1	taxa					1846:1849	Both these bacterial taxa	1825:1849	Both these bacterial taxa	1825:1849	Both these bacterial taxa include acetate-producing bacteria, and a positive correlation between the acetate-to-propionate ratio and the abundance of Pseudoramibacter-Eubacterium was observed.
34218911	7	83	theme	Amplicon	987:994	arg1	sequencing					996:1005	Amplicon sequencing	987:1005	Amplicon sequencing	987:1005	Amplicon sequencing and real-time PCR quantification of the 16S rRNA gene confirmed that the abundance of butyrate-producing bacteria (i.e., Butyrivibrio group and Megasphaera elsdenii) did not differ between the control and LAC10 groups.
34218911	3	84	theme	Holstein	406:413	arg1	calves					420:425	Thirty Holstein bull calves	399:425	Thirty Holstein bull calves	399:425	Thirty Holstein bull calves were divided into 2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis.
34218911	1	85	from	effect	114:119	arg1	performance					173:183	growth performance	166:183	growth performance	166:183	In our previous studies, we revealed the effect of lactose inclusion in calf starters on the growth performance and gut development of calves.
34218911	1	85	from	effect	114:119	arg1	development					193:203	gut development	189:203	gut development	189:203	In our previous studies, we revealed the effect of lactose inclusion in calf starters on the growth performance and gut development of calves.
34218911	1	85	from	effect	114:119	arg1	starters					150:157	calf starters	145:157	calf starters	145:157	In our previous studies, we revealed the effect of lactose inclusion in calf starters on the growth performance and gut development of calves.
34218911	9	86	theme	spp	1584:1586	arg1	increase					1560:1567	an increase	1557:1567	an increase of Mitsuokella spp	1557:1586	These findings suggest that the lactate production can be elevated by an increase of Mitsuokella spp.
34218911	7	87	theme	16S	1047:1049	arg1	rRNA					1051:1054	16S rRNA	1047:1054	the 16S rRNA gene	1043:1059	Amplicon sequencing and real-time PCR quantification of the 16S rRNA gene confirmed that the abundance of butyrate-producing bacteria (i.e., Butyrivibrio group and Megasphaera elsdenii) did not differ between the control and LAC10 groups.
34218911	2	88	theme	rumen	330:334	arg1	fermentation					336:347	rumen fermentation	330:347	rumen fermentation	330:347	We conducted the present study as a follow-up study to identify the shift in rumen microbiota and its relation to rumen fermentation when calves are fed a lactose-containing starter.
34218911	13	89	located	observed	2234:2241	arg2	increase					2181:2188	the increase	2177:2188	the increase in the proportion of rumen acetate that was observed in our previous study	2177:2263	Therefore, the higher abundance of Coriobacteriaceae, Mitsuokella spp., and Pseudoramibacter-Eubacterium in the rumen of lactose-fed calves partially explains the increase in the proportion of rumen acetate that was observed in our previous study.
34218911	13	89	located	observed	2234:2241	arg1	study					2259:2263	our previous study	2246:2263	our previous study	2246:2263	Therefore, the higher abundance of Coriobacteriaceae, Mitsuokella spp., and Pseudoramibacter-Eubacterium in the rumen of lactose-fed calves partially explains the increase in the proportion of rumen acetate that was observed in our previous study.
34218911	13	90	theme	previous	2250:2257	arg1	study					2259:2263	our previous study	2246:2263	our previous study	2246:2263	Therefore, the higher abundance of Coriobacteriaceae, Mitsuokella spp., and Pseudoramibacter-Eubacterium in the rumen of lactose-fed calves partially explains the increase in the proportion of rumen acetate that was observed in our previous study.
34218911	10	91	dep	and	1589:1591	arg1	converted					1598:1606	converted	1598:1606	converted	1598:1606	and then converted into butyrate, not propionate, since the proportion of propionate was lower in lactose-fed calves.
34218911	10	91	dep	and	1589:1591	arg1	then					1593:1596	then	1593:1596	then	1593:1596	and then converted into butyrate, not propionate, since the proportion of propionate was lower in lactose-fed calves.
34218911	13	92	theme	acetate	2217:2223	arg1	proportion					2197:2206	the proportion	2193:2206	the proportion of rumen acetate	2193:2223	Therefore, the higher abundance of Coriobacteriaceae, Mitsuokella spp., and Pseudoramibacter-Eubacterium in the rumen of lactose-fed calves partially explains the increase in the proportion of rumen acetate that was observed in our previous study.
34218911	7	93	theme	butyrate-producing	1093:1110	arg1	bacteria					1112:1119	butyrate-producing bacteria	1093:1119	butyrate-producing bacteria (i.e., Butyrivibrio group and Megasphaera elsdenii)	1093:1171	Amplicon sequencing and real-time PCR quantification of the 16S rRNA gene confirmed that the abundance of butyrate-producing bacteria (i.e., Butyrivibrio group and Megasphaera elsdenii) did not differ between the control and LAC10 groups.
34218911	4	94	theme	respective	662:671	arg1	libitum					699:705	their respective treatment calf starter ad libitum	656:705	their respective treatment calf starter ad libitum	656:705	All calves were fed their respective treatment calf starter ad libitum from d 7, and kleingrass hay from d 35.
34218911	7	95	dep	group	1141:1145	arg1	i.e.					1122:1125	i.e.	1122:1125	i.e.	1122:1125	Amplicon sequencing and real-time PCR quantification of the 16S rRNA gene confirmed that the abundance of butyrate-producing bacteria (i.e., Butyrivibrio group and Megasphaera elsdenii) did not differ between the control and LAC10 groups.
34218911	4	96	theme	calf	683:686	arg1	libitum					699:705	their respective treatment calf starter ad libitum	656:705	their respective treatment calf starter ad libitum	656:705	All calves were fed their respective treatment calf starter ad libitum from d 7, and kleingrass hay from d 35.
34218911	3	97	theme	starter	452:458	arg1	groups					470:475	2 calf starter treatment groups	445:475	2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis	445:633	Thirty Holstein bull calves were divided into 2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis.
34218911	3	97	theme	starter	452:458	arg1	starter					494:500	texturized calf starter	478:500	texturized calf starter (i.e., control; n = 15)	478:524	Thirty Holstein bull calves were divided into 2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis.
34218911	3	97	theme	starter	452:458	arg1	starter					534:540	calf starter	529:540	calf starter	529:540	Thirty Holstein bull calves were divided into 2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis.
34218911	13	98	theme	higher	2033:2038	arg1	abundance					2040:2048	the higher abundance	2029:2048	the higher abundance of Coriobacteriaceae, Mitsuokella spp., and Pseudoramibacter-Eubacterium in the rumen of lactose-fed calves	2029:2156	Therefore, the higher abundance of Coriobacteriaceae, Mitsuokella spp., and Pseudoramibacter-Eubacterium in the rumen of lactose-fed calves partially explains the increase in the proportion of rumen acetate that was observed in our previous study.
34218911	6	99	theme	feeding	915:921	arg1	effect					897:902	no apparent effect	885:902	no apparent effect of lactose feeding on the α-diversity and overall composition of rumen microbiota	885:984	There was no apparent effect of lactose feeding on the α-diversity and overall composition of rumen microbiota.
34218911	13	100	from	increase	2181:2188	arg1	proportion					2197:2206	the proportion	2193:2206	the proportion of rumen acetate	2193:2223	Therefore, the higher abundance of Coriobacteriaceae, Mitsuokella spp., and Pseudoramibacter-Eubacterium in the rumen of lactose-fed calves partially explains the increase in the proportion of rumen acetate that was observed in our previous study.
34218911	7	101	theme	gene	1056:1059	arg1	quantification					1025:1038	real-time PCR quantification	1011:1038	real-time PCR quantification	1011:1038	Amplicon sequencing and real-time PCR quantification of the 16S rRNA gene confirmed that the abundance of butyrate-producing bacteria (i.e., Butyrivibrio group and Megasphaera elsdenii) did not differ between the control and LAC10 groups.
34218911	7	101	theme	gene	1056:1059	arg1	sequencing					996:1005	Amplicon sequencing	987:1005	Amplicon sequencing	987:1005	Amplicon sequencing and real-time PCR quantification of the 16S rRNA gene confirmed that the abundance of butyrate-producing bacteria (i.e., Butyrivibrio group and Megasphaera elsdenii) did not differ between the control and LAC10 groups.
34218911	1	102	dep	performance	173:183	arg1	the					162:164	the	162:164	the	162:164	In our previous studies, we revealed the effect of lactose inclusion in calf starters on the growth performance and gut development of calves.
34218911	3	103	theme	texturized	478:487	arg1	groups					470:475	2 calf starter treatment groups	445:475	2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis	445:633	Thirty Holstein bull calves were divided into 2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis.
34218911	3	103	theme	texturized	478:487	arg1	starter					494:500	texturized calf starter	478:500	texturized calf starter (i.e., control; n = 15)	478:524	Thirty Holstein bull calves were divided into 2 calf starter treatment groups: texturized calf starter (i.e., control; n = 15) or calf starter in which starch was replaced with lactose at 10% (i.e., LAC10; n = 15) on a dry matter basis.
34218911	8	104	theme	control	1461:1467	arg1	groups					1479:1484	the control and LAC10 groups	1457:1484	groups	1479:1484	Conversely, the relative abundance of Mitsuokella spp., which produce lactate, succinate, and acetate, was significantly higher in the rumen of calves that were fed lactose, whereas the lactate concentration did not differ between the control and LAC10 groups.
32278557	6	0	dep	rennet	1157:1162	arg1	%					1174:1174	73 and 26%	1165:1174	%	1174:1174	A similar variability of milk coagulation properties was observed with both rennets, with the exception of greater variability of curd firmness at 30 min after the addition of vegetal rennet compared with animal rennet (73 and 26%, respectively).
32278557	11	1	theme	buffalo	1829:1835	arg1	milk					1837:1840	buffalo milk	1829:1840	buffalo milk	1829:1840	We conclude that vegetal rennet has the capacity to coagulate buffalo milk, achieving a similar curd firmness to that of animal rennet at 60 min.
32278557	3	2	theme	samples	648:654	arg1	composition					628:638	Chemical composition	619:638	Chemical composition of milk samples	619:654	Chemical composition of milk samples was predicted by MilkoScan (Foss Analytics, Hillerød, Denmark) calibrated with specific buffalo standards.
32278557	8	3	theme	0.66	1489:1492	arg1	±					1487:1487	±	1487:1487	±	1487:1487	Thirty minutes after rennet addition, curds were almost twice as firm in animal as in vegetal rennet (difference of 23.92 ± 0.66 mm).
32278557	2	4	dep	animal	533:538	arg1	%					560:560	25%	558:560	25%	558:560	Milk coagulation properties of 1,261 buffalo bulk milk samples collected during milk quality testing were assessed by lactodynamography using commercial animal (75% chymosin and 25% bovine pepsin) and vegetal (Cynara cardunculus) rennets.
32278557	2	4	dep	animal	533:538	arg1	%					543:543	75% chymosin and 25% bovine pepsin	541:574	%	543:543	Milk coagulation properties of 1,261 buffalo bulk milk samples collected during milk quality testing were assessed by lactodynamography using commercial animal (75% chymosin and 25% bovine pepsin) and vegetal (Cynara cardunculus) rennets.
32278557	2	5	theme	bovine	562:567	arg1	pepsin					569:574	bovine pepsin	562:574	bovine pepsin	562:574	Milk coagulation properties of 1,261 buffalo bulk milk samples collected during milk quality testing were assessed by lactodynamography using commercial animal (75% chymosin and 25% bovine pepsin) and vegetal (Cynara cardunculus) rennets.
32278557	10	6	theme	first	1687:1691	arg1	min					1696:1698	the first 30 min	1683:1698	the first 30 min of analysis	1683:1710	Moreover, when using animal rennet, 99.52% of samples started coagulating within the first 30 min of analysis, whereas only 70.42% did so when using vegetal rennet.
32278557	2	7	theme	Cynara	590:595	arg1	cardunculus					597:607	Cynara cardunculus	590:607	Cynara cardunculus	590:607	Milk coagulation properties of 1,261 buffalo bulk milk samples collected during milk quality testing were assessed by lactodynamography using commercial animal (75% chymosin and 25% bovine pepsin) and vegetal (Cynara cardunculus) rennets.
32278557	6	8	theme	curd	1075:1078	arg1	firmness					1080:1087	curd firmness	1075:1087	curd firmness at 30 min after the addition of vegetal rennet compared with animal rennet (73 and 26%, respectively)	1075:1189	A similar variability of milk coagulation properties was observed with both rennets, with the exception of greater variability of curd firmness at 30 min after the addition of vegetal rennet compared with animal rennet (73 and 26%, respectively).
32278557	6	9	theme	animal	1150:1155	arg1	rennet					1157:1162	animal rennet	1150:1162	animal rennet (73 and 26%, respectively)	1150:1189	A similar variability of milk coagulation properties was observed with both rennets, with the exception of greater variability of curd firmness at 30 min after the addition of vegetal rennet compared with animal rennet (73 and 26%, respectively).
32278557	2	10	dep	vegetal	581:587	arg1	cardunculus					597:607	Cynara cardunculus	590:607	Cynara cardunculus	590:607	Milk coagulation properties of 1,261 buffalo bulk milk samples collected during milk quality testing were assessed by lactodynamography using commercial animal (75% chymosin and 25% bovine pepsin) and vegetal (Cynara cardunculus) rennets.
32278557	2	11	theme	vegetal	581:587	arg1	rennets					610:616	commercial animal (75% chymosin and 25% bovine pepsin) and vegetal (Cynara cardunculus) rennets	522:616	commercial animal (75% chymosin and 25% bovine pepsin) and vegetal (Cynara cardunculus) rennets	522:616	Milk coagulation properties of 1,261 buffalo bulk milk samples collected during milk quality testing were assessed by lactodynamography using commercial animal (75% chymosin and 25% bovine pepsin) and vegetal (Cynara cardunculus) rennets.
32278557	6	12	theme	variability	1060:1070	arg1	exception					1039:1047	the exception	1035:1047	the exception of greater variability of curd firmness at 30 min after the addition of vegetal rennet compared with animal rennet (73 and 26%, respectively)	1035:1189	A similar variability of milk coagulation properties was observed with both rennets, with the exception of greater variability of curd firmness at 30 min after the addition of vegetal rennet compared with animal rennet (73 and 26%, respectively).
32278557	10	13	theme	analysis	1703:1710	arg1	min					1696:1698	the first 30 min	1683:1698	the first 30 min of analysis	1683:1710	Moreover, when using animal rennet, 99.52% of samples started coagulating within the first 30 min of analysis, whereas only 70.42% did so when using vegetal rennet.
32278557	1	14	theme	rennet	356:361	arg1	capacity					325:332	clotting capacity	316:332	clotting capacity of vegetal and animal rennet in buffalo milk	316:377	Given consumer interest in Mozzarella di latte di Bufala and other cheeses, and the growing interest of the cheese industry in offering products adequate for lactovegetarian consumers, this study aimed to compare clotting capacity of vegetal and animal rennet in buffalo milk.
32278557	1	15	theme	consumer	109:116	arg1	interest					118:125	consumer interest	109:125	consumer interest in Mozzarella di latte di Bufala and other cheeses	109:176	Given consumer interest in Mozzarella di latte di Bufala and other cheeses, and the growing interest of the cheese industry in offering products adequate for lactovegetarian consumers, this study aimed to compare clotting capacity of vegetal and animal rennet in buffalo milk.
32278557	2	16	theme	animal	533:538	arg1	rennets					610:616	commercial animal (75% chymosin and 25% bovine pepsin) and vegetal (Cynara cardunculus) rennets	522:616	commercial animal (75% chymosin and 25% bovine pepsin) and vegetal (Cynara cardunculus) rennets	522:616	Milk coagulation properties of 1,261 buffalo bulk milk samples collected during milk quality testing were assessed by lactodynamography using commercial animal (75% chymosin and 25% bovine pepsin) and vegetal (Cynara cardunculus) rennets.
32278557	12	17	theme	Bufala	2033:2038	arg1	characteristics					1964:1978	the sensory characteristics	1952:1978	the sensory characteristics	1952:1978	Further studies are needed to evaluate the sensory characteristics and consumer acceptability of Mozzarella di latte di Bufala processed with vegetal rennet.
32278557	12	17	theme	Bufala	2033:2038	arg1	acceptability					1993:2005	consumer acceptability	1984:2005	consumer acceptability	1984:2005	Further studies are needed to evaluate the sensory characteristics and consumer acceptability of Mozzarella di latte di Bufala processed with vegetal rennet.
32278557	6	18	theme	rennet	1129:1134	arg1	addition					1109:1116	the addition	1105:1116	the addition of vegetal rennet compared with animal rennet (73 and 26%, respectively)	1105:1189	A similar variability of milk coagulation properties was observed with both rennets, with the exception of greater variability of curd firmness at 30 min after the addition of vegetal rennet compared with animal rennet (73 and 26%, respectively).
32278557	1	19	theme	buffalo	366:372	arg1	milk					374:377	buffalo milk	366:377	buffalo milk	366:377	Given consumer interest in Mozzarella di latte di Bufala and other cheeses, and the growing interest of the cheese industry in offering products adequate for lactovegetarian consumers, this study aimed to compare clotting capacity of vegetal and animal rennet in buffalo milk.
32278557	1	20	theme	growing	187:193	arg1	interest					195:202	the growing interest	183:202	the growing interest of the cheese industry in offering products adequate for lactovegetarian consumers	183:285	Given consumer interest in Mozzarella di latte di Bufala and other cheeses, and the growing interest of the cheese industry in offering products adequate for lactovegetarian consumers, this study aimed to compare clotting capacity of vegetal and animal rennet in buffalo milk.
32278557	1	21	from	interest	195:202	arg1	Bufala					153:158	Bufala	153:158	Bufala	153:158	Given consumer interest in Mozzarella di latte di Bufala and other cheeses, and the growing interest of the cheese industry in offering products adequate for lactovegetarian consumers, this study aimed to compare clotting capacity of vegetal and animal rennet in buffalo milk.
32278557	1	21	from	interest	195:202	arg1	cheeses					170:176	other cheeses	164:176	other cheeses	164:176	Given consumer interest in Mozzarella di latte di Bufala and other cheeses, and the growing interest of the cheese industry in offering products adequate for lactovegetarian consumers, this study aimed to compare clotting capacity of vegetal and animal rennet in buffalo milk.
32278557	1	21	from	interest	195:202	arg1	products					239:246	offering products	230:246	offering products adequate for lactovegetarian consumers	230:285	Given consumer interest in Mozzarella di latte di Bufala and other cheeses, and the growing interest of the cheese industry in offering products adequate for lactovegetarian consumers, this study aimed to compare clotting capacity of vegetal and animal rennet in buffalo milk.
32278557	11	22	contain	has	1799:1801	arg2	capacity					1807:1814	the capacity to coagulate buffalo milk	1803:1840	the capacity to coagulate buffalo milk	1803:1840	We conclude that vegetal rennet has the capacity to coagulate buffalo milk, achieving a similar curd firmness to that of animal rennet at 60 min.
32278557	11	22	contain	has	1799:1801	arg1	rennet					1792:1797	vegetal rennet	1784:1797	vegetal rennet	1784:1797	We conclude that vegetal rennet has the capacity to coagulate buffalo milk, achieving a similar curd firmness to that of animal rennet at 60 min.
32278557	4	23	theme	paired	835:840	arg1	t-test					842:847	a paired t-test	833:847	a paired t-test	833:847	Rennet effect (animal versus vegetal) was statistically analyzed with a paired t-test.
32278557	7	24	theme	plant	1215:1219	arg1	rennet					1221:1226	plant rennet	1215:1226	plant rennet	1215:1226	On average, when using plant rennet, milk started to coagulate and reached the 20-mm coagulum 12 ± 0.22 min and 1.9 ± 0.20 min, respectively, later than with animal rennet.
32278557	8	25	dep	minutes	1372:1378	arg1	addition					1393:1400	rennet addition	1386:1400	rennet addition	1386:1400	Thirty minutes after rennet addition, curds were almost twice as firm in animal as in vegetal rennet (difference of 23.92 ± 0.66 mm).
32278557	5	26	theme	lactose	868:874	arg1	contents					876:883	Fat, protein, and lactose contents	850:883	Fat, protein, and lactose contents of milk samples	850:899	Fat, protein, and lactose contents of milk samples were 7.94%, 4.52%, and 4.80%, respectively.
32278557	3	27	theme	buffalo	744:750	arg1	standards					752:760	specific buffalo standards	735:760	specific buffalo standards	735:760	Chemical composition of milk samples was predicted by MilkoScan (Foss Analytics, Hillerød, Denmark) calibrated with specific buffalo standards.
32278557	0	28	theme	coagulation	48:58	arg1	traits					60:65	milk coagulation traits	43:65	milk coagulation traits in Mediterranean buffalo bulk milk	43:100	Effects of animal versus vegetal rennet on milk coagulation traits in Mediterranean buffalo bulk milk.
32278557	2	29	theme	quality	465:471	arg1	testing					473:479	milk quality testing	460:479	milk quality testing	460:479	Milk coagulation properties of 1,261 buffalo bulk milk samples collected during milk quality testing were assessed by lactodynamography using commercial animal (75% chymosin and 25% bovine pepsin) and vegetal (Cynara cardunculus) rennets.
32278557	1	30	theme	clotting	316:323	arg1	capacity					325:332	clotting capacity	316:332	clotting capacity of vegetal and animal rennet in buffalo milk	316:377	Given consumer interest in Mozzarella di latte di Bufala and other cheeses, and the growing interest of the cheese industry in offering products adequate for lactovegetarian consumers, this study aimed to compare clotting capacity of vegetal and animal rennet in buffalo milk.
32278557	9	31	theme	1.21	1541:1544	arg1	±					1546:1546	±	1546:1546	±	1546:1546	However, curd firmness at 60 min was only 1.21 ± 0.39 mm thicker with vegetal than with animal rennet.
32278557	0	32	from	Effects	0:6	arg1	traits					60:65	milk coagulation traits	43:65	milk coagulation traits in Mediterranean buffalo bulk milk	43:100	Effects of animal versus vegetal rennet on milk coagulation traits in Mediterranean buffalo bulk milk.
32278557	10	33	theme	vegetal	1751:1757	arg1	rennet					1759:1764	vegetal rennet	1751:1764	vegetal rennet	1751:1764	Moreover, when using animal rennet, 99.52% of samples started coagulating within the first 30 min of analysis, whereas only 70.42% did so when using vegetal rennet.
32278557	0	34	theme	buffalo	84:90	arg1	milk					97:100	Mediterranean buffalo bulk milk	70:100	Mediterranean buffalo bulk milk	70:100	Effects of animal versus vegetal rennet on milk coagulation traits in Mediterranean buffalo bulk milk.
32278557	6	35	theme	firmness	1080:1087	arg1	variability					1060:1070	greater variability	1052:1070	greater variability of curd firmness at 30 min after the addition of vegetal rennet compared with animal rennet (73 and 26%, respectively)	1052:1189	A similar variability of milk coagulation properties was observed with both rennets, with the exception of greater variability of curd firmness at 30 min after the addition of vegetal rennet compared with animal rennet (73 and 26%, respectively).
32278557	9	36	theme	0.39	1548:1551	arg1	±					1546:1546	±	1546:1546	±	1546:1546	However, curd firmness at 60 min was only 1.21 ± 0.39 mm thicker with vegetal than with animal rennet.
32278557	1	37	from	interest	118:125	arg1	Bufala					153:158	Bufala	153:158	Bufala	153:158	Given consumer interest in Mozzarella di latte di Bufala and other cheeses, and the growing interest of the cheese industry in offering products adequate for lactovegetarian consumers, this study aimed to compare clotting capacity of vegetal and animal rennet in buffalo milk.
32278557	1	37	from	interest	118:125	arg1	cheeses					170:176	other cheeses	164:176	other cheeses	164:176	Given consumer interest in Mozzarella di latte di Bufala and other cheeses, and the growing interest of the cheese industry in offering products adequate for lactovegetarian consumers, this study aimed to compare clotting capacity of vegetal and animal rennet in buffalo milk.
32278557	1	37	from	interest	118:125	arg1	products					239:246	offering products	230:246	offering products adequate for lactovegetarian consumers	230:285	Given consumer interest in Mozzarella di latte di Bufala and other cheeses, and the growing interest of the cheese industry in offering products adequate for lactovegetarian consumers, this study aimed to compare clotting capacity of vegetal and animal rennet in buffalo milk.
32278557	6	38	theme	coagulation	975:985	arg1	properties					987:996	milk coagulation properties	970:996	milk coagulation properties	970:996	A similar variability of milk coagulation properties was observed with both rennets, with the exception of greater variability of curd firmness at 30 min after the addition of vegetal rennet compared with animal rennet (73 and 26%, respectively).
32278557	2	39	theme	bulk	425:428	arg1	samples					435:441	1,261 buffalo bulk milk samples	411:441	1,261 buffalo bulk milk samples collected during milk quality testing	411:479	Milk coagulation properties of 1,261 buffalo bulk milk samples collected during milk quality testing were assessed by lactodynamography using commercial animal (75% chymosin and 25% bovine pepsin) and vegetal (Cynara cardunculus) rennets.
32278557	12	40	theme	sensory	1956:1962	arg1	characteristics					1964:1978	the sensory characteristics	1952:1978	the sensory characteristics	1952:1978	Further studies are needed to evaluate the sensory characteristics and consumer acceptability of Mozzarella di latte di Bufala processed with vegetal rennet.
32278557	1	41	theme	adequate	248:255	arg1	products					239:246	offering products	230:246	offering products adequate for lactovegetarian consumers	230:285	Given consumer interest in Mozzarella di latte di Bufala and other cheeses, and the growing interest of the cheese industry in offering products adequate for lactovegetarian consumers, this study aimed to compare clotting capacity of vegetal and animal rennet in buffalo milk.
32278557	11	42	theme	curd	1863:1866	arg1	firmness					1868:1875	a similar curd firmness	1853:1875	a similar curd firmness to that of animal rennet	1853:1900	We conclude that vegetal rennet has the capacity to coagulate buffalo milk, achieving a similar curd firmness to that of animal rennet at 60 min.
32278557	12	43	theme	Further	1913:1919	arg1	studies					1921:1927	Further studies	1913:1927	Further studies	1913:1927	Further studies are needed to evaluate the sensory characteristics and consumer acceptability of Mozzarella di latte di Bufala processed with vegetal rennet.
32278557	0	44	theme	animal	11:16	arg1	rennet					33:38	animal versus vegetal rennet	11:38	animal versus vegetal rennet	11:38	Effects of animal versus vegetal rennet on milk coagulation traits in Mediterranean buffalo bulk milk.
32278557	1	45	theme	lactovegetarian	261:275	arg1	consumers					277:285	lactovegetarian consumers	261:285	lactovegetarian consumers	261:285	Given consumer interest in Mozzarella di latte di Bufala and other cheeses, and the growing interest of the cheese industry in offering products adequate for lactovegetarian consumers, this study aimed to compare clotting capacity of vegetal and animal rennet in buffalo milk.
32278557	7	46	theme	20-mm	1271:1275	arg1	min					1296:1298	the 20-mm coagulum 12 ± 0.22 min	1267:1298	the 20-mm coagulum 12 ± 0.22 min	1267:1298	On average, when using plant rennet, milk started to coagulate and reached the 20-mm coagulum 12 ± 0.22 min and 1.9 ± 0.20 min, respectively, later than with animal rennet.
32278557	5	47	theme	samples	893:899	arg1	contents					876:883	Fat, protein, and lactose contents	850:883	Fat, protein, and lactose contents of milk samples	850:899	Fat, protein, and lactose contents of milk samples were 7.94%, 4.52%, and 4.80%, respectively.
32278557	6	48	theme	similar	947:953	arg1	variability					955:965	A similar variability	945:965	A similar variability of milk coagulation properties	945:996	A similar variability of milk coagulation properties was observed with both rennets, with the exception of greater variability of curd firmness at 30 min after the addition of vegetal rennet compared with animal rennet (73 and 26%, respectively).
32278557	2	49	theme	Milk	380:383	arg1	properties					397:406	Milk coagulation properties	380:406	Milk coagulation properties of 1,261 buffalo bulk milk samples collected during milk quality testing	380:479	Milk coagulation properties of 1,261 buffalo bulk milk samples collected during milk quality testing were assessed by lactodynamography using commercial animal (75% chymosin and 25% bovine pepsin) and vegetal (Cynara cardunculus) rennets.
32278557	0	50	theme	vegetal	25:31	arg1	rennet					33:38	animal versus vegetal rennet	11:38	animal versus vegetal rennet	11:38	Effects of animal versus vegetal rennet on milk coagulation traits in Mediterranean buffalo bulk milk.
32278557	1	51	from	capacity	325:332	arg1	milk					374:377	buffalo milk	366:377	buffalo milk	366:377	Given consumer interest in Mozzarella di latte di Bufala and other cheeses, and the growing interest of the cheese industry in offering products adequate for lactovegetarian consumers, this study aimed to compare clotting capacity of vegetal and animal rennet in buffalo milk.
32278557	2	52	theme	samples	435:441	arg1	properties					397:406	Milk coagulation properties	380:406	Milk coagulation properties of 1,261 buffalo bulk milk samples collected during milk quality testing	380:479	Milk coagulation properties of 1,261 buffalo bulk milk samples collected during milk quality testing were assessed by lactodynamography using commercial animal (75% chymosin and 25% bovine pepsin) and vegetal (Cynara cardunculus) rennets.
32278557	3	53	theme	milk	643:646	arg1	samples					648:654	milk samples	643:654	milk samples	643:654	Chemical composition of milk samples was predicted by MilkoScan (Foss Analytics, Hillerød, Denmark) calibrated with specific buffalo standards.
32278557	8	54	theme	vegetal	1451:1457	arg1	rennet					1459:1464	vegetal rennet	1451:1464	vegetal rennet (difference of 23.92 ± 0.66 mm)	1451:1496	Thirty minutes after rennet addition, curds were almost twice as firm in animal as in vegetal rennet (difference of 23.92 ± 0.66 mm).
32278557	10	55	theme	animal	1623:1628	arg1	rennet					1630:1635	animal rennet	1623:1635	animal rennet	1623:1635	Moreover, when using animal rennet, 99.52% of samples started coagulating within the first 30 min of analysis, whereas only 70.42% did so when using vegetal rennet.
32278557	4	56	theme	Rennet	763:768	arg1	effect					770:775	Rennet effect	763:775	Rennet effect (animal versus vegetal)	763:799	Rennet effect (animal versus vegetal) was statistically analyzed with a paired t-test.
32278557	2	57	dep	%	543:543	arg1	pepsin					569:574	bovine pepsin	562:574	bovine pepsin	562:574	Milk coagulation properties of 1,261 buffalo bulk milk samples collected during milk quality testing were assessed by lactodynamography using commercial animal (75% chymosin and 25% bovine pepsin) and vegetal (Cynara cardunculus) rennets.
32278557	2	57	dep	%	543:543	arg1	chymosin					545:552	chymosin	545:552	chymosin	545:552	Milk coagulation properties of 1,261 buffalo bulk milk samples collected during milk quality testing were assessed by lactodynamography using commercial animal (75% chymosin and 25% bovine pepsin) and vegetal (Cynara cardunculus) rennets.
32278557	10	58	theme	samples	1648:1654	arg1	samples					1648:1654	samples	1648:1654	samples	1648:1654	Moreover, when using animal rennet, 99.52% of samples started coagulating within the first 30 min of analysis, whereas only 70.42% did so when using vegetal rennet.
32278557	10	58	theme	samples	1648:1654	arg1	%					1643:1643	99.52%	1638:1643	99.52% of samples	1638:1654	Moreover, when using animal rennet, 99.52% of samples started coagulating within the first 30 min of analysis, whereas only 70.42% did so when using vegetal rennet.
32278557	6	59	theme	26	1172:1173	arg1	%					1174:1174	73 and 26%	1165:1174	%	1174:1174	A similar variability of milk coagulation properties was observed with both rennets, with the exception of greater variability of curd firmness at 30 min after the addition of vegetal rennet compared with animal rennet (73 and 26%, respectively).
32278557	1	60	theme	industry	218:225	arg1	interest					118:125	consumer interest	109:125	consumer interest in Mozzarella di latte di Bufala and other cheeses	109:176	Given consumer interest in Mozzarella di latte di Bufala and other cheeses, and the growing interest of the cheese industry in offering products adequate for lactovegetarian consumers, this study aimed to compare clotting capacity of vegetal and animal rennet in buffalo milk.
32278557	1	60	theme	industry	218:225	arg1	interest					195:202	the growing interest	183:202	the growing interest of the cheese industry in offering products adequate for lactovegetarian consumers	183:285	Given consumer interest in Mozzarella di latte di Bufala and other cheeses, and the growing interest of the cheese industry in offering products adequate for lactovegetarian consumers, this study aimed to compare clotting capacity of vegetal and animal rennet in buffalo milk.
32278557	6	61	theme	73	1165:1166	arg1	%					1174:1174	73 and 26%	1165:1174	%	1174:1174	A similar variability of milk coagulation properties was observed with both rennets, with the exception of greater variability of curd firmness at 30 min after the addition of vegetal rennet compared with animal rennet (73 and 26%, respectively).
32278557	1	62	theme	offering	230:237	arg1	products					239:246	offering products	230:246	offering products adequate for lactovegetarian consumers	230:285	Given consumer interest in Mozzarella di latte di Bufala and other cheeses, and the growing interest of the cheese industry in offering products adequate for lactovegetarian consumers, this study aimed to compare clotting capacity of vegetal and animal rennet in buffalo milk.
32278557	7	63	theme	±	1289:1289	arg1	min					1296:1298	the 20-mm coagulum 12 ± 0.22 min	1267:1298	the 20-mm coagulum 12 ± 0.22 min	1267:1298	On average, when using plant rennet, milk started to coagulate and reached the 20-mm coagulum 12 ± 0.22 min and 1.9 ± 0.20 min, respectively, later than with animal rennet.
32278557	8	64	theme	mm	1494:1495	arg1	difference					1467:1476	difference	1467:1476	difference of 23.92 ± 0.66 mm	1467:1495	Thirty minutes after rennet addition, curds were almost twice as firm in animal as in vegetal rennet (difference of 23.92 ± 0.66 mm).
32278557	11	65	theme	animal	1888:1893	arg1	rennet					1895:1900	animal rennet	1888:1900	animal rennet	1888:1900	We conclude that vegetal rennet has the capacity to coagulate buffalo milk, achieving a similar curd firmness to that of animal rennet at 60 min.
32278557	11	66	theme	similar	1855:1861	arg1	firmness					1868:1875	a similar curd firmness	1853:1875	a similar curd firmness to that of animal rennet	1853:1900	We conclude that vegetal rennet has the capacity to coagulate buffalo milk, achieving a similar curd firmness to that of animal rennet at 60 min.
32278557	7	67	theme	coagulum	1277:1284	arg1	min					1296:1298	the 20-mm coagulum 12 ± 0.22 min	1267:1298	the 20-mm coagulum 12 ± 0.22 min	1267:1298	On average, when using plant rennet, milk started to coagulate and reached the 20-mm coagulum 12 ± 0.22 min and 1.9 ± 0.20 min, respectively, later than with animal rennet.
32278557	11	68	dep	to	1816:1817	arg1	coagulate					1819:1827	coagulate	1819:1827	to coagulate buffalo milk	1816:1840	We conclude that vegetal rennet has the capacity to coagulate buffalo milk, achieving a similar curd firmness to that of animal rennet at 60 min.
32278557	1	69	theme	other	164:168	arg1	cheeses					170:176	other cheeses	164:176	other cheeses	164:176	Given consumer interest in Mozzarella di latte di Bufala and other cheeses, and the growing interest of the cheese industry in offering products adequate for lactovegetarian consumers, this study aimed to compare clotting capacity of vegetal and animal rennet in buffalo milk.
32278557	6	70	theme	greater	1052:1058	arg1	variability					1060:1070	greater variability	1052:1070	greater variability of curd firmness at 30 min after the addition of vegetal rennet compared with animal rennet (73 and 26%, respectively)	1052:1189	A similar variability of milk coagulation properties was observed with both rennets, with the exception of greater variability of curd firmness at 30 min after the addition of vegetal rennet compared with animal rennet (73 and 26%, respectively).
32278557	8	71	from	firm	1430:1433	arg1	animal					1438:1443	animal	1438:1443	animal as in vegetal rennet (difference of 23.92 ± 0.66 mm)	1438:1496	Thirty minutes after rennet addition, curds were almost twice as firm in animal as in vegetal rennet (difference of 23.92 ± 0.66 mm).
32278557	11	72	theme	vegetal	1784:1790	arg1	rennet					1792:1797	vegetal rennet	1784:1797	vegetal rennet	1784:1797	We conclude that vegetal rennet has the capacity to coagulate buffalo milk, achieving a similar curd firmness to that of animal rennet at 60 min.
32278557	2	73	theme	commercial	522:531	arg1	rennets					610:616	commercial animal (75% chymosin and 25% bovine pepsin) and vegetal (Cynara cardunculus) rennets	522:616	commercial animal (75% chymosin and 25% bovine pepsin) and vegetal (Cynara cardunculus) rennets	522:616	Milk coagulation properties of 1,261 buffalo bulk milk samples collected during milk quality testing were assessed by lactodynamography using commercial animal (75% chymosin and 25% bovine pepsin) and vegetal (Cynara cardunculus) rennets.
32278557	0	74	from	traits	60:65	arg1	milk					97:100	Mediterranean buffalo bulk milk	70:100	Mediterranean buffalo bulk milk	70:100	Effects of animal versus vegetal rennet on milk coagulation traits in Mediterranean buffalo bulk milk.
32278557	6	75	theme	vegetal	1121:1127	arg1	rennet					1129:1134	vegetal rennet	1121:1134	vegetal rennet compared with animal rennet (73 and 26%, respectively)	1121:1189	A similar variability of milk coagulation properties was observed with both rennets, with the exception of greater variability of curd firmness at 30 min after the addition of vegetal rennet compared with animal rennet (73 and 26%, respectively).
32278557	3	76	theme	specific	735:742	arg1	standards					752:760	specific buffalo standards	735:760	specific buffalo standards	735:760	Chemical composition of milk samples was predicted by MilkoScan (Foss Analytics, Hillerød, Denmark) calibrated with specific buffalo standards.
32278557	5	77	theme	protein	855:861	arg1	contents					876:883	Fat, protein, and lactose contents	850:883	Fat, protein, and lactose contents of milk samples	850:899	Fat, protein, and lactose contents of milk samples were 7.94%, 4.52%, and 4.80%, respectively.
32278557	9	78	theme	curd	1508:1511	arg1	firmness					1513:1520	curd firmness	1508:1520	curd firmness at 60 min	1508:1530	However, curd firmness at 60 min was only 1.21 ± 0.39 mm thicker with vegetal than with animal rennet.
32278557	8	79	from	animal	1438:1443	arg1	firm					1430:1433	firm	1430:1433	firm	1430:1433	Thirty minutes after rennet addition, curds were almost twice as firm in animal as in vegetal rennet (difference of 23.92 ± 0.66 mm).
32278557	12	80	theme	consumer	1984:1991	arg1	acceptability					1993:2005	consumer acceptability	1984:2005	consumer acceptability	1984:2005	Further studies are needed to evaluate the sensory characteristics and consumer acceptability of Mozzarella di latte di Bufala processed with vegetal rennet.
32278557	8	81	dep	rennet	1459:1464	arg1	difference					1467:1476	difference	1467:1476	difference of 23.92 ± 0.66 mm	1467:1495	Thirty minutes after rennet addition, curds were almost twice as firm in animal as in vegetal rennet (difference of 23.92 ± 0.66 mm).
32278557	6	82	from	min	1095:1097	arg1	variability					1060:1070	greater variability	1052:1070	greater variability of curd firmness at 30 min after the addition of vegetal rennet compared with animal rennet (73 and 26%, respectively)	1052:1189	A similar variability of milk coagulation properties was observed with both rennets, with the exception of greater variability of curd firmness at 30 min after the addition of vegetal rennet compared with animal rennet (73 and 26%, respectively).
32278557	6	82	from	min	1095:1097	arg1	firmness					1080:1087	curd firmness	1075:1087	curd firmness at 30 min after the addition of vegetal rennet compared with animal rennet (73 and 26%, respectively)	1075:1189	A similar variability of milk coagulation properties was observed with both rennets, with the exception of greater variability of curd firmness at 30 min after the addition of vegetal rennet compared with animal rennet (73 and 26%, respectively).
32278557	0	83	theme	Mediterranean	70:82	arg1	milk					97:100	Mediterranean buffalo bulk milk	70:100	Mediterranean buffalo bulk milk	70:100	Effects of animal versus vegetal rennet on milk coagulation traits in Mediterranean buffalo bulk milk.
32278557	5	84	theme	Fat	850:852	arg1	contents					876:883	Fat, protein, and lactose contents	850:883	Fat, protein, and lactose contents of milk samples	850:899	Fat, protein, and lactose contents of milk samples were 7.94%, 4.52%, and 4.80%, respectively.
32278557	2	85	theme	milk	460:463	arg1	testing					473:479	milk quality testing	460:479	milk quality testing	460:479	Milk coagulation properties of 1,261 buffalo bulk milk samples collected during milk quality testing were assessed by lactodynamography using commercial animal (75% chymosin and 25% bovine pepsin) and vegetal (Cynara cardunculus) rennets.
32278557	0	86	theme	bulk	92:95	arg1	milk					97:100	Mediterranean buffalo bulk milk	70:100	Mediterranean buffalo bulk milk	70:100	Effects of animal versus vegetal rennet on milk coagulation traits in Mediterranean buffalo bulk milk.
32278557	1	87	theme	vegetal	337:343	arg1	rennet					356:361	vegetal and animal rennet	337:361	vegetal and animal rennet	337:361	Given consumer interest in Mozzarella di latte di Bufala and other cheeses, and the growing interest of the cheese industry in offering products adequate for lactovegetarian consumers, this study aimed to compare clotting capacity of vegetal and animal rennet in buffalo milk.
32278557	6	88	theme	properties	987:996	arg1	variability					955:965	A similar variability	945:965	A similar variability of milk coagulation properties	945:996	A similar variability of milk coagulation properties was observed with both rennets, with the exception of greater variability of curd firmness at 30 min after the addition of vegetal rennet compared with animal rennet (73 and 26%, respectively).
32278557	1	89	theme	animal	349:354	arg1	rennet					356:361	vegetal and animal rennet	337:361	vegetal and animal rennet	337:361	Given consumer interest in Mozzarella di latte di Bufala and other cheeses, and the growing interest of the cheese industry in offering products adequate for lactovegetarian consumers, this study aimed to compare clotting capacity of vegetal and animal rennet in buffalo milk.
32278557	6	90	theme	milk	970:973	arg1	properties					987:996	milk coagulation properties	970:996	milk coagulation properties	970:996	A similar variability of milk coagulation properties was observed with both rennets, with the exception of greater variability of curd firmness at 30 min after the addition of vegetal rennet compared with animal rennet (73 and 26%, respectively).
32278557	2	91	theme	buffalo	417:423	arg1	samples					435:441	1,261 buffalo bulk milk samples	411:441	1,261 buffalo bulk milk samples collected during milk quality testing	411:479	Milk coagulation properties of 1,261 buffalo bulk milk samples collected during milk quality testing were assessed by lactodynamography using commercial animal (75% chymosin and 25% bovine pepsin) and vegetal (Cynara cardunculus) rennets.
32278557	9	92	from	min	1528:1530	arg1	firmness					1513:1520	curd firmness	1508:1520	curd firmness at 60 min	1508:1530	However, curd firmness at 60 min was only 1.21 ± 0.39 mm thicker with vegetal than with animal rennet.
32278557	5	93	theme	milk	888:891	arg1	samples					893:899	milk samples	888:899	milk samples	888:899	Fat, protein, and lactose contents of milk samples were 7.94%, 4.52%, and 4.80%, respectively.
32278557	8	94	theme	rennet	1386:1391	arg1	addition					1393:1400	rennet addition	1386:1400	rennet addition	1386:1400	Thirty minutes after rennet addition, curds were almost twice as firm in animal as in vegetal rennet (difference of 23.92 ± 0.66 mm).
32278557	6	95	from	variability	1060:1070	arg1	min					1095:1097	30 min	1092:1097	30 min after the addition of vegetal rennet compared with animal rennet (73 and 26%, respectively)	1092:1189	A similar variability of milk coagulation properties was observed with both rennets, with the exception of greater variability of curd firmness at 30 min after the addition of vegetal rennet compared with animal rennet (73 and 26%, respectively).
32278557	2	96	theme	coagulation	385:395	arg1	properties					397:406	Milk coagulation properties	380:406	Milk coagulation properties of 1,261 buffalo bulk milk samples collected during milk quality testing	380:479	Milk coagulation properties of 1,261 buffalo bulk milk samples collected during milk quality testing were assessed by lactodynamography using commercial animal (75% chymosin and 25% bovine pepsin) and vegetal (Cynara cardunculus) rennets.
32278557	0	97	theme	rennet	33:38	arg1	Effects					0:6	Effects	0:6	Effects of animal versus vegetal rennet on milk coagulation traits in Mediterranean buffalo bulk milk.	0:101	Effects of animal versus vegetal rennet on milk coagulation traits in Mediterranean buffalo bulk milk.
32278557	9	98	theme	animal	1587:1592	arg1	rennet					1594:1599	animal rennet	1587:1599	animal rennet	1587:1599	However, curd firmness at 60 min was only 1.21 ± 0.39 mm thicker with vegetal than with animal rennet.
32278557	0	99	theme	milk	43:46	arg1	traits					60:65	milk coagulation traits	43:65	milk coagulation traits in Mediterranean buffalo bulk milk	43:100	Effects of animal versus vegetal rennet on milk coagulation traits in Mediterranean buffalo bulk milk.
32278557	7	100	theme	animal	1350:1355	arg1	rennet					1357:1362	animal rennet	1350:1362	animal rennet	1350:1362	On average, when using plant rennet, milk started to coagulate and reached the 20-mm coagulum 12 ± 0.22 min and 1.9 ± 0.20 min, respectively, later than with animal rennet.
32278557	2	101	theme	milk	430:433	arg1	samples					435:441	1,261 buffalo bulk milk samples	411:441	1,261 buffalo bulk milk samples collected during milk quality testing	411:479	Milk coagulation properties of 1,261 buffalo bulk milk samples collected during milk quality testing were assessed by lactodynamography using commercial animal (75% chymosin and 25% bovine pepsin) and vegetal (Cynara cardunculus) rennets.
32278557	12	102	theme	vegetal	2055:2061	arg1	rennet					2063:2068	vegetal rennet	2055:2068	vegetal rennet	2055:2068	Further studies are needed to evaluate the sensory characteristics and consumer acceptability of Mozzarella di latte di Bufala processed with vegetal rennet.
32278557	3	103	theme	Chemical	619:626	arg1	composition					628:638	Chemical composition	619:638	Chemical composition of milk samples	619:654	Chemical composition of milk samples was predicted by MilkoScan (Foss Analytics, Hillerød, Denmark) calibrated with specific buffalo standards.
32278557	4	104	dep	effect	770:775	arg1	vegetal					792:798	vegetal	792:798	vegetal	792:798	Rennet effect (animal versus vegetal) was statistically analyzed with a paired t-test.
32278557	4	104	dep	effect	770:775	arg1	animal					778:783	animal	778:783	animal	778:783	Rennet effect (animal versus vegetal) was statistically analyzed with a paired t-test.
32278557	8	105	theme	23.92	1481:1485	arg1	±					1487:1487	±	1487:1487	±	1487:1487	Thirty minutes after rennet addition, curds were almost twice as firm in animal as in vegetal rennet (difference of 23.92 ± 0.66 mm).
32278557	1	106	theme	cheese	211:216	arg1	industry					218:225	the cheese industry	207:225	the cheese industry	207:225	Given consumer interest in Mozzarella di latte di Bufala and other cheeses, and the growing interest of the cheese industry in offering products adequate for lactovegetarian consumers, this study aimed to compare clotting capacity of vegetal and animal rennet in buffalo milk.
33058982	0	0	theme	chemistry	82:90	arg1	Impact					72:77	Impact	72:77	Biodegradable double cross-linked chitosan hydrogels for drug delivery: Impact of chemistry on rheological and pharmacological performance.	0:138	Biodegradable double cross-linked chitosan hydrogels for drug delivery: Impact of chemistry on rheological and pharmacological performance.
33058982	2	1	theme	ionic	446:450	arg1	derivative					412:421	a diiodo-trehalose derivative	393:421	a diiodo-trehalose derivative (ITrh)	393:428	Citric acid and a diiodo-trehalose derivative (ITrh) were the chosen ionic and covalent cross-linker, respectively.
33058982	2	1	theme	ionic	446:450	arg1	acid					384:387	Citric acid	377:387	Citric acid	377:387	Citric acid and a diiodo-trehalose derivative (ITrh) were the chosen ionic and covalent cross-linker, respectively.
33058982	2	1	theme	ionic	446:450	arg1	cross-linker					465:476	the chosen ionic and covalent cross-linker	435:476	the chosen ionic and covalent cross-linker	435:476	Citric acid and a diiodo-trehalose derivative (ITrh) were the chosen ionic and covalent cross-linker, respectively.
33058982	5	2	theme	cov-XrL	833:839	arg1	degree					823:828	degree	823:828	degree of cov-XrL	823:839	The impact of CTS concentration and degree of cov-XrL on rheological parameters were examined by means of an experimental model design and marked differences were found between the materials.
33058982	5	2	theme	cov-XrL	833:839	arg1	concentration					805:817	CTS concentration	801:817	CTS concentration	801:817	The impact of CTS concentration and degree of cov-XrL on rheological parameters were examined by means of an experimental model design and marked differences were found between the materials.
33058982	9	3	theme	simple	1369:1374	arg1	pathway					1396:1402	a simple and straightforward pathway	1367:1402	a simple and straightforward pathway to design fully biodegradable, tailor-made controlled drug delivery systems with improved rheological properties	1367:1515	The formulations presented herein provides a simple and straightforward pathway to design fully biodegradable, tailor-made controlled drug delivery systems with improved rheological properties.
33058982	2	4	theme	chosen	439:444	arg1	derivative					412:421	a diiodo-trehalose derivative	393:421	a diiodo-trehalose derivative (ITrh)	393:428	Citric acid and a diiodo-trehalose derivative (ITrh) were the chosen ionic and covalent cross-linker, respectively.
33058982	2	4	theme	chosen	439:444	arg1	acid					384:387	Citric acid	377:387	Citric acid	377:387	Citric acid and a diiodo-trehalose derivative (ITrh) were the chosen ionic and covalent cross-linker, respectively.
33058982	2	4	theme	chosen	439:444	arg1	cross-linker					465:476	the chosen ionic and covalent cross-linker	435:476	the chosen ionic and covalent cross-linker	435:476	Citric acid and a diiodo-trehalose derivative (ITrh) were the chosen ionic and covalent cross-linker, respectively.
33058982	1	5	from	impact	168:173	arg1	properties					243:252	the properties	239:252	the properties of chitosan-based (CTS) hydrogels	239:286	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	6	6	theme	high	1038:1041	arg1	concentrations					1047:1060	high CTS concentrations	1038:1060	high CTS concentrations	1038:1060	Hydrogels with maximum elastic properties were achieved at high CTS concentrations and high degrees of cov-XrL.
33058982	9	7	theme	delivery	1463:1470	arg1	systems					1472:1478	fully biodegradable, tailor-made controlled drug delivery systems	1414:1478	fully biodegradable, tailor-made controlled drug delivery systems with improved rheological properties	1414:1515	The formulations presented herein provides a simple and straightforward pathway to design fully biodegradable, tailor-made controlled drug delivery systems with improved rheological properties.
33058982	0	8	theme	rheological	95:105	arg1	performance					127:137	rheological and pharmacological performance	95:137	rheological and pharmacological performance	95:137	Biodegradable double cross-linked chitosan hydrogels for drug delivery: Impact of chemistry on rheological and pharmacological performance.
33058982	0	9	link	cross-linked	21:32	arg1	hydrogels					43:51	Biodegradable double cross-linked chitosan hydrogels	0:51	Biodegradable double cross-linked chitosan hydrogels for drug delivery: Impact of chemistry on rheological and pharmacological performance.	0:138	Biodegradable double cross-linked chitosan hydrogels for drug delivery: Impact of chemistry on rheological and pharmacological performance.
33058982	5	10	theme	experimental	896:907	arg1	design					915:920	an experimental model design	893:920	an experimental model design	893:920	The impact of CTS concentration and degree of cov-XrL on rheological parameters were examined by means of an experimental model design and marked differences were found between the materials.
33058982	1	11	theme	chitosan-based	257:270	arg1	hydrogels					278:286	chitosan-based (CTS) hydrogels	257:286	chitosan-based (CTS) hydrogels	257:286	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	1	11	theme	chitosan-based	257:270	arg1	CTS					273:275	CTS	273:275	CTS	273:275	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	9	12	theme	improved	1485:1492	arg1	properties					1506:1515	improved rheological properties	1485:1515	improved rheological properties	1485:1515	The formulations presented herein provides a simple and straightforward pathway to design fully biodegradable, tailor-made controlled drug delivery systems with improved rheological properties.
33058982	7	13	from	%	1225:1225	arg1	composition					1198:1208	formulation composition	1186:1208	formulation composition (from 17% to 40% in 72 h)	1186:1234	DCNa-loaded formulations displayed well-controlled drug-release profiles strongly dependent on formulation composition (from 17% to 40% in 72 h).
33058982	7	13	from	%	1225:1225	arg1	72 h					1230:1233	72 h	1230:1233	72 h	1230:1233	DCNa-loaded formulations displayed well-controlled drug-release profiles strongly dependent on formulation composition (from 17% to 40% in 72 h).
33058982	5	14	theme	model	909:913	arg1	design					915:920	an experimental model design	893:920	an experimental model design	893:920	The impact of CTS concentration and degree of cov-XrL on rheological parameters were examined by means of an experimental model design and marked differences were found between the materials.
33058982	6	15	theme	high	1066:1069	arg1	degrees					1071:1077	high degrees	1066:1077	high degrees of cov-XrL	1066:1088	Hydrogels with maximum elastic properties were achieved at high CTS concentrations and high degrees of cov-XrL.
33058982	0	16	theme	pharmacological	111:125	arg1	performance					127:137	rheological and pharmacological performance	95:137	rheological and pharmacological performance	95:137	Biodegradable double cross-linked chitosan hydrogels for drug delivery: Impact of chemistry on rheological and pharmacological performance.
33058982	1	17	theme	model	340:344	arg1	DCNa					370:373	the model drug diclofenac sodium (DCNa)	336:374	the model drug diclofenac sodium (DCNa)	336:374	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	6	18	theme	CTS	1043:1045	arg1	concentrations					1047:1060	high CTS concentrations	1038:1060	high CTS concentrations	1038:1060	Hydrogels with maximum elastic properties were achieved at high CTS concentrations and high degrees of cov-XrL.
33058982	8	19	theme	drug	1310:1313	arg1	release					1315:1321	drug release	1310:1321	drug release	1310:1321	Surprisingly, higher degrees of covalent cross-linking led to a boost in drug release.
33058982	1	20	theme	dual	178:181	arg1	cov-XrL					227:233	cov-XrL	227:233	cov-XrL	227:233	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	1	20	theme	dual	178:181	arg1	ion-XrL					215:221	ion-XrL	215:221	ion-XrL	215:221	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	1	20	theme	dual	178:181	arg1	cross-links					202:212	dual ionic and covalent cross-links	178:212	dual ionic and covalent cross-links (ion-XrL and cov-XrL)	178:234	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	7	21	theme	DCNa-loaded	1091:1101	arg1	formulations					1103:1114	DCNa-loaded formulations	1091:1114	DCNa-loaded formulations	1091:1114	DCNa-loaded formulations displayed well-controlled drug-release profiles strongly dependent on formulation composition (from 17% to 40% in 72 h).
33058982	1	22	theme	drug	346:349	arg1	DCNa					370:373	the model drug diclofenac sodium (DCNa)	336:374	the model drug diclofenac sodium (DCNa)	336:374	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	4	23	theme	hydrogels	776:784	arg1	formation					749:757	the formation	745:757	the formation of biodegradable hydrogels	745:784	As far as the authors are aware, this is the first time that a trehalose derivative has been used as a covalent cross-linker in the formation of biodegradable hydrogels.
33058982	9	24	theme	rheological	1494:1504	arg1	properties					1506:1515	improved rheological properties	1485:1515	improved rheological properties	1485:1515	The formulations presented herein provides a simple and straightforward pathway to design fully biodegradable, tailor-made controlled drug delivery systems with improved rheological properties.
33058982	3	25	theme	novel	497:501	arg1	hydrogels					503:511	The novel hydrogels	493:511	The novel hydrogels	493:511	The novel hydrogels completely disintegrated within 96 h by means of a hydrolysis process mediated by the enzyme trehalase.
33058982	1	26	theme	ionic	183:187	arg1	cov-XrL					227:233	cov-XrL	227:233	cov-XrL	227:233	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	1	26	theme	ionic	183:187	arg1	ion-XrL					215:221	ion-XrL	215:221	ion-XrL	215:221	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	1	26	theme	ionic	183:187	arg1	cross-links					202:212	dual ionic and covalent cross-links	178:212	dual ionic and covalent cross-links (ion-XrL and cov-XrL)	178:234	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	0	27	theme	double	14:19	arg1	hydrogels					43:51	Biodegradable double cross-linked chitosan hydrogels	0:51	Biodegradable double cross-linked chitosan hydrogels for drug delivery: Impact of chemistry on rheological and pharmacological performance.	0:138	Biodegradable double cross-linked chitosan hydrogels for drug delivery: Impact of chemistry on rheological and pharmacological performance.
33058982	1	28	theme	diclofenac	351:360	arg1	DCNa					370:373	the model drug diclofenac sodium (DCNa)	336:374	the model drug diclofenac sodium (DCNa)	336:374	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	4	29	dep	time	668:671	arg1	used					710:713	used	710:713	has been used as a covalent cross-linker in the formation of biodegradable hydrogels	701:784	As far as the authors are aware, this is the first time that a trehalose derivative has been used as a covalent cross-linker in the formation of biodegradable hydrogels.
33058982	5	30	theme	concentration	805:817	arg1	impact					791:796	The impact	787:796	The impact of CTS concentration and degree of cov-XrL on rheological parameters	787:865	The impact of CTS concentration and degree of cov-XrL on rheological parameters were examined by means of an experimental model design and marked differences were found between the materials.
33058982	0	31	theme	Biodegradable	0:12	arg1	hydrogels					43:51	Biodegradable double cross-linked chitosan hydrogels	0:51	Biodegradable double cross-linked chitosan hydrogels for drug delivery: Impact of chemistry on rheological and pharmacological performance.	0:138	Biodegradable double cross-linked chitosan hydrogels for drug delivery: Impact of chemistry on rheological and pharmacological performance.
33058982	9	32	dep	biodegradable	1420:1432	arg1	tailor-made					1435:1445	tailor-made	1435:1445	tailor-made	1435:1445	The formulations presented herein provides a simple and straightforward pathway to design fully biodegradable, tailor-made controlled drug delivery systems with improved rheological properties.
33058982	9	33	with	systems	1472:1478	arg1	properties					1506:1515	improved rheological properties	1485:1515	improved rheological properties	1485:1515	The formulations presented herein provides a simple and straightforward pathway to design fully biodegradable, tailor-made controlled drug delivery systems with improved rheological properties.
33058982	9	34	theme	straightforward	1380:1394	arg1	pathway					1396:1402	a simple and straightforward pathway	1367:1402	a simple and straightforward pathway to design fully biodegradable, tailor-made controlled drug delivery systems with improved rheological properties	1367:1515	The formulations presented herein provides a simple and straightforward pathway to design fully biodegradable, tailor-made controlled drug delivery systems with improved rheological properties.
33058982	1	35	theme	covalent	193:200	arg1	cov-XrL					227:233	cov-XrL	227:233	cov-XrL	227:233	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	1	35	theme	covalent	193:200	arg1	ion-XrL					215:221	ion-XrL	215:221	ion-XrL	215:221	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	1	35	theme	covalent	193:200	arg1	cross-links					202:212	dual ionic and covalent cross-links	178:212	dual ionic and covalent cross-links (ion-XrL and cov-XrL)	178:234	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	0	36	theme	chitosan	34:41	arg1	hydrogels					43:51	Biodegradable double cross-linked chitosan hydrogels	0:51	Biodegradable double cross-linked chitosan hydrogels for drug delivery: Impact of chemistry on rheological and pharmacological performance.	0:138	Biodegradable double cross-linked chitosan hydrogels for drug delivery: Impact of chemistry on rheological and pharmacological performance.
33058982	6	37	with	Hydrogels	979:987	arg1	properties					1010:1019	maximum elastic properties	994:1019	maximum elastic properties	994:1019	Hydrogels with maximum elastic properties were achieved at high CTS concentrations and high degrees of cov-XrL.
33058982	7	38	dep	%	1225:1225	arg1	to					1220:1221	to	1220:1221	to	1220:1221	DCNa-loaded formulations displayed well-controlled drug-release profiles strongly dependent on formulation composition (from 17% to 40% in 72 h).
33058982	1	39	theme	cross-links	202:212	arg1	impact					168:173	the impact	164:173	the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels	164:286	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	1	39	theme	cross-links	202:212	arg1	systems					318:324	eco-friendly drug delivery systems	291:324	eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa)	291:374	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	0	40	theme	cross-linked	21:32	arg1	hydrogels					43:51	Biodegradable double cross-linked chitosan hydrogels	0:51	Biodegradable double cross-linked chitosan hydrogels for drug delivery: Impact of chemistry on rheological and pharmacological performance.	0:138	Biodegradable double cross-linked chitosan hydrogels for drug delivery: Impact of chemistry on rheological and pharmacological performance.
33058982	1	41	theme	hydrogels	278:286	arg1	properties					243:252	the properties	239:252	the properties of chitosan-based (CTS) hydrogels	239:286	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	1	42	theme	sodium	362:367	arg1	DCNa					370:373	the model drug diclofenac sodium (DCNa)	336:374	the model drug diclofenac sodium (DCNa)	336:374	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	4	43	theme	trehalose	680:688	arg1	cross-linker					729:740	a covalent cross-linker	718:740	a covalent cross-linker in the formation of biodegradable hydrogels	718:784	As far as the authors are aware, this is the first time that a trehalose derivative has been used as a covalent cross-linker in the formation of biodegradable hydrogels.
33058982	4	43	theme	trehalose	680:688	arg1	derivative					690:699	a trehalose derivative	678:699	a trehalose derivative	678:699	As far as the authors are aware, this is the first time that a trehalose derivative has been used as a covalent cross-linker in the formation of biodegradable hydrogels.
33058982	6	44	theme	maximum	994:1000	arg1	properties					1010:1019	maximum elastic properties	994:1019	maximum elastic properties	994:1019	Hydrogels with maximum elastic properties were achieved at high CTS concentrations and high degrees of cov-XrL.
33058982	7	45	theme	formulation	1186:1196	arg1	composition					1198:1208	formulation composition	1186:1208	formulation composition (from 17% to 40% in 72 h)	1186:1234	DCNa-loaded formulations displayed well-controlled drug-release profiles strongly dependent on formulation composition (from 17% to 40% in 72 h).
33058982	9	46	theme	controlled	1447:1456	arg1	systems					1472:1478	fully biodegradable, tailor-made controlled drug delivery systems	1414:1478	fully biodegradable, tailor-made controlled drug delivery systems with improved rheological properties	1414:1515	The formulations presented herein provides a simple and straightforward pathway to design fully biodegradable, tailor-made controlled drug delivery systems with improved rheological properties.
33058982	8	47	theme	cross-linking	1278:1290	arg1	degrees					1258:1264	higher degrees	1251:1264	higher degrees of covalent cross-linking	1251:1290	Surprisingly, higher degrees of covalent cross-linking led to a boost in drug release.
33058982	9	48	theme	biodegradable	1420:1432	arg1	systems					1472:1478	fully biodegradable, tailor-made controlled drug delivery systems	1414:1478	fully biodegradable, tailor-made controlled drug delivery systems with improved rheological properties	1414:1515	The formulations presented herein provides a simple and straightforward pathway to design fully biodegradable, tailor-made controlled drug delivery systems with improved rheological properties.
33058982	2	49	theme	Citric	377:382	arg1	derivative					412:421	a diiodo-trehalose derivative	393:421	a diiodo-trehalose derivative (ITrh)	393:428	Citric acid and a diiodo-trehalose derivative (ITrh) were the chosen ionic and covalent cross-linker, respectively.
33058982	2	49	theme	Citric	377:382	arg1	acid					384:387	Citric acid	377:387	Citric acid	377:387	Citric acid and a diiodo-trehalose derivative (ITrh) were the chosen ionic and covalent cross-linker, respectively.
33058982	2	49	theme	Citric	377:382	arg1	cross-linker					465:476	the chosen ionic and covalent cross-linker	435:476	the chosen ionic and covalent cross-linker	435:476	Citric acid and a diiodo-trehalose derivative (ITrh) were the chosen ionic and covalent cross-linker, respectively.
33058982	1	50	theme	eco-friendly	291:302	arg1	impact					168:173	the impact	164:173	the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels	164:286	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	1	50	theme	eco-friendly	291:302	arg1	DDS					327:329	DDS	327:329	DDS	327:329	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	1	50	theme	eco-friendly	291:302	arg1	systems					318:324	eco-friendly drug delivery systems	291:324	eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa)	291:374	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	0	51	from	Impact	72:77	arg1	performance					127:137	rheological and pharmacological performance	95:137	rheological and pharmacological performance	95:137	Biodegradable double cross-linked chitosan hydrogels for drug delivery: Impact of chemistry on rheological and pharmacological performance.
33058982	2	52	theme	diiodo-trehalose	395:410	arg1	derivative					412:421	a diiodo-trehalose derivative	393:421	a diiodo-trehalose derivative (ITrh)	393:428	Citric acid and a diiodo-trehalose derivative (ITrh) were the chosen ionic and covalent cross-linker, respectively.
33058982	2	52	theme	diiodo-trehalose	395:410	arg1	acid					384:387	Citric acid	377:387	Citric acid	377:387	Citric acid and a diiodo-trehalose derivative (ITrh) were the chosen ionic and covalent cross-linker, respectively.
33058982	2	52	theme	diiodo-trehalose	395:410	arg1	ITrh					424:427	ITrh	424:427	ITrh	424:427	Citric acid and a diiodo-trehalose derivative (ITrh) were the chosen ionic and covalent cross-linker, respectively.
33058982	2	52	theme	diiodo-trehalose	395:410	arg1	cross-linker					465:476	the chosen ionic and covalent cross-linker	435:476	the chosen ionic and covalent cross-linker	435:476	Citric acid and a diiodo-trehalose derivative (ITrh) were the chosen ionic and covalent cross-linker, respectively.
33058982	8	53	dep	a	1299:1299	arg1	boost					1301:1305	boost	1301:1305	boost in drug release	1301:1321	Surprisingly, higher degrees of covalent cross-linking led to a boost in drug release.
33058982	8	54	theme	higher	1251:1256	arg1	degrees					1258:1264	higher degrees	1251:1264	higher degrees of covalent cross-linking	1251:1290	Surprisingly, higher degrees of covalent cross-linking led to a boost in drug release.
33058982	1	55	theme	drug	304:307	arg1	impact					168:173	the impact	164:173	the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels	164:286	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	1	55	theme	drug	304:307	arg1	DDS					327:329	DDS	327:329	DDS	327:329	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	1	55	theme	drug	304:307	arg1	systems					318:324	eco-friendly drug delivery systems	291:324	eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa)	291:374	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	4	56	theme	biodegradable	762:774	arg1	hydrogels					776:784	biodegradable hydrogels	762:784	biodegradable hydrogels	762:784	As far as the authors are aware, this is the first time that a trehalose derivative has been used as a covalent cross-linker in the formation of biodegradable hydrogels.
33058982	5	57	from	impact	791:796	arg1	parameters					856:865	rheological parameters	844:865	rheological parameters	844:865	The impact of CTS concentration and degree of cov-XrL on rheological parameters were examined by means of an experimental model design and marked differences were found between the materials.
33058982	5	58	theme	rheological	844:854	arg1	parameters					856:865	rheological parameters	844:865	rheological parameters	844:865	The impact of CTS concentration and degree of cov-XrL on rheological parameters were examined by means of an experimental model design and marked differences were found between the materials.
33058982	0	59	theme	drug	57:60	arg1	delivery					62:69	drug delivery	57:69	drug delivery	57:69	Biodegradable double cross-linked chitosan hydrogels for drug delivery: Impact of chemistry on rheological and pharmacological performance.
33058982	1	60	theme	delivery	309:316	arg1	impact					168:173	the impact	164:173	the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels	164:286	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	1	60	theme	delivery	309:316	arg1	DDS					327:329	DDS	327:329	DDS	327:329	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	1	60	theme	delivery	309:316	arg1	systems					318:324	eco-friendly drug delivery systems	291:324	eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa)	291:374	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	0	61	dep	hydrogels	43:51	arg1	Impact					72:77	Impact	72:77	Biodegradable double cross-linked chitosan hydrogels for drug delivery: Impact of chemistry on rheological and pharmacological performance.	0:138	Biodegradable double cross-linked chitosan hydrogels for drug delivery: Impact of chemistry on rheological and pharmacological performance.
33058982	3	62	theme	enzyme	599:604	arg1	trehalase					606:614	the enzyme trehalase	595:614	the enzyme trehalase	595:614	The novel hydrogels completely disintegrated within 96 h by means of a hydrolysis process mediated by the enzyme trehalase.
33058982	4	63	used	used	710:713	arg2	derivative					690:699	a trehalose derivative	678:699	a trehalose derivative	678:699	As far as the authors are aware, this is the first time that a trehalose derivative has been used as a covalent cross-linker in the formation of biodegradable hydrogels.
33058982	4	63	used	used	710:713	arg2	cross-linker					729:740	a covalent cross-linker	718:740	a covalent cross-linker in the formation of biodegradable hydrogels	718:784	As far as the authors are aware, this is the first time that a trehalose derivative has been used as a covalent cross-linker in the formation of biodegradable hydrogels.
33058982	7	64	theme	well-controlled	1126:1140	arg1	profiles					1155:1162	well-controlled drug-release profiles	1126:1162	well-controlled drug-release profiles strongly dependent on formulation composition (from 17% to 40% in 72 h)	1126:1234	DCNa-loaded formulations displayed well-controlled drug-release profiles strongly dependent on formulation composition (from 17% to 40% in 72 h).
33058982	5	65	theme	marked	926:931	arg1	differences					933:943	marked differences	926:943	marked differences	926:943	The impact of CTS concentration and degree of cov-XrL on rheological parameters were examined by means of an experimental model design and marked differences were found between the materials.
33058982	4	66	theme	covalent	720:727	arg1	cross-linker					729:740	a covalent cross-linker	718:740	a covalent cross-linker in the formation of biodegradable hydrogels	718:784	As far as the authors are aware, this is the first time that a trehalose derivative has been used as a covalent cross-linker in the formation of biodegradable hydrogels.
33058982	4	66	theme	covalent	720:727	arg1	derivative					690:699	a trehalose derivative	678:699	a trehalose derivative	678:699	As far as the authors are aware, this is the first time that a trehalose derivative has been used as a covalent cross-linker in the formation of biodegradable hydrogels.
33058982	3	67	theme	hydrolysis	564:573	arg1	process					575:581	a hydrolysis process	562:581	a hydrolysis process mediated by the enzyme trehalase	562:614	The novel hydrogels completely disintegrated within 96 h by means of a hydrolysis process mediated by the enzyme trehalase.
33058982	6	68	theme	elastic	1002:1008	arg1	properties					1010:1019	maximum elastic properties	994:1019	maximum elastic properties	994:1019	Hydrogels with maximum elastic properties were achieved at high CTS concentrations and high degrees of cov-XrL.
33058982	4	69	theme	first	662:666	arg1	this					650:653	this	650:653	this	650:653	As far as the authors are aware, this is the first time that a trehalose derivative has been used as a covalent cross-linker in the formation of biodegradable hydrogels.
33058982	4	69	theme	first	662:666	arg1	time					668:671	the first time	658:671	the first time that a trehalose derivative has been used as a covalent cross-linker in the formation of biodegradable hydrogels	658:784	As far as the authors are aware, this is the first time that a trehalose derivative has been used as a covalent cross-linker in the formation of biodegradable hydrogels.
33058982	6	70	theme	cov-XrL	1082:1088	arg1	concentrations					1047:1060	high CTS concentrations	1038:1060	high CTS concentrations	1038:1060	Hydrogels with maximum elastic properties were achieved at high CTS concentrations and high degrees of cov-XrL.
33058982	6	70	theme	cov-XrL	1082:1088	arg1	degrees					1071:1077	high degrees	1066:1077	high degrees of cov-XrL	1066:1088	Hydrogels with maximum elastic properties were achieved at high CTS concentrations and high degrees of cov-XrL.
33058982	2	71	theme	covalent	456:463	arg1	derivative					412:421	a diiodo-trehalose derivative	393:421	a diiodo-trehalose derivative (ITrh)	393:428	Citric acid and a diiodo-trehalose derivative (ITrh) were the chosen ionic and covalent cross-linker, respectively.
33058982	2	71	theme	covalent	456:463	arg1	acid					384:387	Citric acid	377:387	Citric acid	377:387	Citric acid and a diiodo-trehalose derivative (ITrh) were the chosen ionic and covalent cross-linker, respectively.
33058982	2	71	theme	covalent	456:463	arg1	cross-linker					465:476	the chosen ionic and covalent cross-linker	435:476	the chosen ionic and covalent cross-linker	435:476	Citric acid and a diiodo-trehalose derivative (ITrh) were the chosen ionic and covalent cross-linker, respectively.
33058982	9	72	theme	drug	1458:1461	arg1	systems					1472:1478	fully biodegradable, tailor-made controlled drug delivery systems	1414:1478	fully biodegradable, tailor-made controlled drug delivery systems with improved rheological properties	1414:1515	The formulations presented herein provides a simple and straightforward pathway to design fully biodegradable, tailor-made controlled drug delivery systems with improved rheological properties.
33058982	1	73	dep	cross-links	202:212	arg1	cov-XrL					227:233	cov-XrL	227:233	cov-XrL	227:233	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	1	73	dep	cross-links	202:212	arg1	ion-XrL					215:221	ion-XrL	215:221	ion-XrL	215:221	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	1	73	dep	cross-links	202:212	arg1	cross-links					202:212	dual ionic and covalent cross-links	178:212	dual ionic and covalent cross-links (ion-XrL and cov-XrL)	178:234	This study investigates the impact of dual ionic and covalent cross-links (ion-XrL and cov-XrL) on the properties of chitosan-based (CTS) hydrogels as eco-friendly drug delivery systems (DDS) for the model drug diclofenac sodium (DCNa).
33058982	4	74	from	cross-linker	729:740	arg1	formation					749:757	the formation	745:757	the formation of biodegradable hydrogels	745:784	As far as the authors are aware, this is the first time that a trehalose derivative has been used as a covalent cross-linker in the formation of biodegradable hydrogels.
33058982	5	75	theme	degree	823:828	arg1	impact					791:796	The impact	787:796	The impact of CTS concentration and degree of cov-XrL on rheological parameters	787:865	The impact of CTS concentration and degree of cov-XrL on rheological parameters were examined by means of an experimental model design and marked differences were found between the materials.
33058982	5	76	theme	CTS	801:803	arg1	concentration					805:817	CTS concentration	801:817	CTS concentration	801:817	The impact of CTS concentration and degree of cov-XrL on rheological parameters were examined by means of an experimental model design and marked differences were found between the materials.
33058982	7	77	theme	%	1218:1218	arg1	%					1225:1225	17% to 40%	1216:1225	17% to 40% in 72 h	1216:1233	DCNa-loaded formulations displayed well-controlled drug-release profiles strongly dependent on formulation composition (from 17% to 40% in 72 h).
33058982	7	78	theme	dependent	1173:1181	arg1	profiles					1155:1162	well-controlled drug-release profiles	1126:1162	well-controlled drug-release profiles strongly dependent on formulation composition (from 17% to 40% in 72 h)	1126:1234	DCNa-loaded formulations displayed well-controlled drug-release profiles strongly dependent on formulation composition (from 17% to 40% in 72 h).
33058982	7	79	theme	drug-release	1142:1153	arg1	profiles					1155:1162	well-controlled drug-release profiles	1126:1162	well-controlled drug-release profiles strongly dependent on formulation composition (from 17% to 40% in 72 h)	1126:1234	DCNa-loaded formulations displayed well-controlled drug-release profiles strongly dependent on formulation composition (from 17% to 40% in 72 h).
33058982	8	80	theme	covalent	1269:1276	arg1	cross-linking					1278:1290	covalent cross-linking	1269:1290	covalent cross-linking	1269:1290	Surprisingly, higher degrees of covalent cross-linking led to a boost in drug release.
33712429	8	0	theme	Tibetan	2008:2014	arg1	He					2048:2049	J. Li, D. Chen, B. Yu, J. He	2022:2049	J. Li, D. Chen, B. Yu, J. He	2022:2049	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	8	0	theme	Tibetan	2008:2014	arg1	pigs					2016:2019	grazing Tibetan pigs	2000:2019	grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507)	2000:2135	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	5	1	theme	fungal	1006:1011	arg1	community					1013:1021	the fungal community	1002:1021	the fungal community in the colon of the pigs	1002:1046	Sequencing based on an Illumina HiSeq 2500 platform targeting the internal transcribed spacer 1 region showed that the fungal community in the colon of the pigs responded to DCHO in the order of MOS, AM/AP, and NSP.
33712429	11	2	theme	clear	2628:2632	arg1	alteration					2634:2643	a clear alteration	2626:2643	a clear alteration of the colonic fungal community in pigs triggered by different compositions of DCHO simulated by varied concentrations of starch, nonstarch polysaccharides (NSP), and oligosaccharides	2626:2827	Here, we show a clear alteration of the colonic fungal community in pigs triggered by different compositions of DCHO simulated by varied concentrations of starch, nonstarch polysaccharides (NSP), and oligosaccharides.
33712429	3	3	theme	community	501:509	arg1	response					470:477	the response	466:477	the response of the colonic fungal community to different compositions of DCHO in a pig model	466:558	Here, we report the response of the colonic fungal community to different compositions of DCHO in a pig model.
33712429	3	4	theme	colonic	486:492	arg1	community					501:509	the colonic fungal community	482:509	the colonic fungal community	482:509	Here, we report the response of the colonic fungal community to different compositions of DCHO in a pig model.
33712429	7	5	theme	gut	1700:1702	arg1	microorganisms					1704:1717	gut microorganisms	1700:1717	gut microorganisms	1700:1717	Our study provides direct evidence for the relationship between the composition of DCHO and the fungal community in the colon of pigs, which is helpful to understand the function of gut microorganisms in pigs.IMPORTANCE Although fungi are a large group of microorganisms along with bacteria and archaea in the gut of monogastric animals, the nutritional significance of fungi has been ignored for a long time.
33712429	2	6	with	relationship	378:389	arg1	composition					400:410	the composition	396:410	the composition of intestinal fungi	396:430	A large number of studies have shown that dietary carbohydrates (DCHO) are related to the bacterial community in the gut, but their relationship with the composition of intestinal fungi is still unknown.
33712429	12	7	from	involvement	2866:2876	arg1	utilization					2905:2915	the utilization	2901:2915	the utilization of nutrients in monogastric animals	2901:2951	Our results highlight the potential involvement of intestinal fungi in the utilization of nutrients in monogastric animals.
33712429	1	8	theme	energy	197:202	arg1	source					204:209	the most important energy source	178:209	the most important energy source in the diet of humans and animals	178:243	Carbohydrates represent the most important energy source in the diet of humans and animals.
33712429	11	9	theme	polysaccharides	2785:2799	arg1	concentrations					2749:2762	varied concentrations	2742:2762	varied concentrations of starch, nonstarch polysaccharides (NSP), and oligosaccharides	2742:2827	Here, we show a clear alteration of the colonic fungal community in pigs triggered by different compositions of DCHO simulated by varied concentrations of starch, nonstarch polysaccharides (NSP), and oligosaccharides.
33712429	3	10	theme	different	514:522	arg1	compositions					524:535	different compositions	514:535	different compositions of DCHO in a pig model	514:558	Here, we report the response of the colonic fungal community to different compositions of DCHO in a pig model.
33712429	5	11	theme	MOS	1082:1084	arg1	order					1073:1077	the order	1069:1077	the order of MOS, AM/AP, and NSP	1069:1100	Sequencing based on an Illumina HiSeq 2500 platform targeting the internal transcribed spacer 1 region showed that the fungal community in the colon of the pigs responded to DCHO in the order of MOS, AM/AP, and NSP.
33712429	4	12	theme	body	754:757	arg1	weight					759:764	1,200 mg/kg body weight	742:764	1,200 mg/kg body weight	742:764	Three factors, ratio (2:1, 1:1, and 1:2) of amylose to amylopectin (AM/AP), level of nonstarch polysaccharides (NSP; 1%, 2%, and 3%), and mannan-oligosaccharide (MOS; 400, 800, and 1,200 mg/kg body weight), were considered according to an L9 (34) orthogonal design to form nine diets with different carbohydrate compositions.
33712429	9	13	theme	intestinal	2476:2485	arg1	fungi					2487:2491	intestinal fungi	2476:2491	intestinal fungi	2476:2491	These groundbreaking findings indicate a potential relationship between intestinal fungi and the utilization of DCHO.
33712429	3	14	theme	pig	550:552	arg1	model					554:558	a pig model	548:558	a pig model	548:558	Here, we report the response of the colonic fungal community to different compositions of DCHO in a pig model.
33712429	8	15	theme	J.	2045:2046	arg1	https					2096:2100	https	2096:2100	https	2096:2100	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	8	15	theme	J.	2045:2046	arg1	He					2048:2049	J. Li, D. Chen, B. Yu, J. He	2022:2049	J. Li, D. Chen, B. Yu, J. He	2022:2049	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	8	15	theme	J.	2045:2046	arg1	pigs					2016:2019	grazing Tibetan pigs	2000:2019	grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507)	2000:2135	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	8	15	theme	J.	2045:2046	arg1	13:509-521					2078:2087	Microb Biotechnol 13:509-521	2060:2087	Microb Biotechnol 13:509-521	2060:2087	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	8	15	theme	J.	2045:2046	arg1	2020					2090:2093	2020	2090:2093	2020	2090:2093	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	8	15	theme	J.	2045:2046	arg1	al.					2055:2057	et al.	2052:2057	et al.	2052:2057	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	11	16	from	alteration	2634:2643	arg1	pigs					2680:2683	pigs	2680:2683	pigs triggered by different compositions of DCHO simulated by varied concentrations of starch, nonstarch polysaccharides (NSP), and oligosaccharides	2680:2827	Here, we show a clear alteration of the colonic fungal community in pigs triggered by different compositions of DCHO simulated by varied concentrations of starch, nonstarch polysaccharides (NSP), and oligosaccharides.
33712429	11	17	theme	nonstarch	2775:2783	arg1	polysaccharides					2785:2799	nonstarch polysaccharides	2775:2799	nonstarch polysaccharides (NSP)	2775:2805	Here, we show a clear alteration of the colonic fungal community in pigs triggered by different compositions of DCHO simulated by varied concentrations of starch, nonstarch polysaccharides (NSP), and oligosaccharides.
33712429	11	17	theme	nonstarch	2775:2783	arg1	NSP					2802:2804	NSP	2802:2804	NSP	2802:2804	Here, we show a clear alteration of the colonic fungal community in pigs triggered by different compositions of DCHO simulated by varied concentrations of starch, nonstarch polysaccharides (NSP), and oligosaccharides.
33712429	1	18	from	source	204:209	arg1	diet					218:221	the diet	214:221	the diet of humans and animals	214:243	Carbohydrates represent the most important energy source in the diet of humans and animals.
33712429	6	19	from	colon	1503:1507	arg1	degradation					1476:1486	the degradation	1472:1486	the degradation of DCHO in the colon of pigs	1472:1515	A large part of some low-abundance fungal genera correlated with the composition of DCHO, represented by Saccharomycopsis, Mrakia, Wallemia, Cantharellus, Eurotium, Solicoccozyma, and Penicillium, were also associated with the concentration of glucose and fructose, as well as the activity of β-d-glucosidase in the colonic digesta, suggesting a role of these fungi in the degradation of DCHO in the colon of pigs.
33712429	12	20	theme	potential	2856:2864	arg1	involvement					2866:2876	the potential involvement	2852:2876	the potential involvement of intestinal fungi in the utilization of nutrients in monogastric animals	2852:2951	Our results highlight the potential involvement of intestinal fungi in the utilization of nutrients in monogastric animals.
33712429	12	21	theme	monogastric	2933:2943	arg1	animals					2945:2951	monogastric animals	2933:2951	monogastric animals	2933:2951	Our results highlight the potential involvement of intestinal fungi in the utilization of nutrients in monogastric animals.
33712429	2	22	theme	large	248:252	arg1	number					254:259	A large number	246:259	A large number of studies	246:270	A large number of studies have shown that dietary carbohydrates (DCHO) are related to the bacterial community in the gut, but their relationship with the composition of intestinal fungi is still unknown.
33712429	7	23	from	composition	1586:1596	arg1	colon					1638:1642	the colon	1634:1642	the colon	1634:1642	Our study provides direct evidence for the relationship between the composition of DCHO and the fungal community in the colon of pigs, which is helpful to understand the function of gut microorganisms in pigs.IMPORTANCE Although fungi are a large group of microorganisms along with bacteria and archaea in the gut of monogastric animals, the nutritional significance of fungi has been ignored for a long time.
33712429	7	23	from	composition	1586:1596	arg1	helpful					1662:1668	helpful	1662:1668	helpful	1662:1668	Our study provides direct evidence for the relationship between the composition of DCHO and the fungal community in the colon of pigs, which is helpful to understand the function of gut microorganisms in pigs.IMPORTANCE Although fungi are a large group of microorganisms along with bacteria and archaea in the gut of monogastric animals, the nutritional significance of fungi has been ignored for a long time.
33712429	6	24	theme	β-d-glucosidase	1396:1410	arg1	activity					1384:1391	the activity	1380:1391	the activity of β-d-glucosidase in the colonic digesta	1380:1433	A large part of some low-abundance fungal genera correlated with the composition of DCHO, represented by Saccharomycopsis, Mrakia, Wallemia, Cantharellus, Eurotium, Solicoccozyma, and Penicillium, were also associated with the concentration of glucose and fructose, as well as the activity of β-d-glucosidase in the colonic digesta, suggesting a role of these fungi in the degradation of DCHO in the colon of pigs.
33712429	6	25	theme	genera	1145:1150	arg1	part					1111:1114	A large part	1103:1114	A large part of some low-abundance fungal genera	1103:1150	A large part of some low-abundance fungal genera correlated with the composition of DCHO, represented by Saccharomycopsis, Mrakia, Wallemia, Cantharellus, Eurotium, Solicoccozyma, and Penicillium, were also associated with the concentration of glucose and fructose, as well as the activity of β-d-glucosidase in the colonic digesta, suggesting a role of these fungi in the degradation of DCHO in the colon of pigs.
33712429	5	26	theme	HiSeq	919:923	arg1	platform					930:937	an Illumina HiSeq 2500 platform	907:937	an Illumina HiSeq 2500 platform targeting the internal transcribed spacer 1 region	907:988	Sequencing based on an Illumina HiSeq 2500 platform targeting the internal transcribed spacer 1 region showed that the fungal community in the colon of the pigs responded to DCHO in the order of MOS, AM/AP, and NSP.
33712429	12	27	from	utilization	2905:2915	arg1	animals					2945:2951	monogastric animals	2933:2951	monogastric animals	2933:2951	Our results highlight the potential involvement of intestinal fungi in the utilization of nutrients in monogastric animals.
33712429	8	28	theme	Microb	2060:2065	arg1	He					2048:2049	J. Li, D. Chen, B. Yu, J. He	2022:2049	J. Li, D. Chen, B. Yu, J. He	2022:2049	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	8	28	theme	Microb	2060:2065	arg1	13:509-521					2078:2087	Microb Biotechnol 13:509-521	2060:2087	Microb Biotechnol 13:509-521	2060:2087	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	4	29	theme	amylose	605:611	arg1	ratio					576:580	ratio	576:580	ratio (2:1, 1:1, and 1:2) of amylose to amylopectin (AM/AP), level of nonstarch polysaccharides (NSP; 1%, 2%, and 3%), and mannan-oligosaccharide (MOS; 400, 800, and 1,200 mg/kg body weight)	576:765	Three factors, ratio (2:1, 1:1, and 1:2) of amylose to amylopectin (AM/AP), level of nonstarch polysaccharides (NSP; 1%, 2%, and 3%), and mannan-oligosaccharide (MOS; 400, 800, and 1,200 mg/kg body weight), were considered according to an L9 (34) orthogonal design to form nine diets with different carbohydrate compositions.
33712429	4	29	theme	amylose	605:611	arg1	factors					567:573	Three factors	561:573	Three factors	561:573	Three factors, ratio (2:1, 1:1, and 1:2) of amylose to amylopectin (AM/AP), level of nonstarch polysaccharides (NSP; 1%, 2%, and 3%), and mannan-oligosaccharide (MOS; 400, 800, and 1,200 mg/kg body weight), were considered according to an L9 (34) orthogonal design to form nine diets with different carbohydrate compositions.
33712429	0	30	theme	Carbohydrate	72:83	arg1	Composition					85:95	Dietary Carbohydrate Composition	64:95	Dietary Carbohydrate Composition	64:95	The Nutritional Significance of Intestinal Fungi: Alteration of Dietary Carbohydrate Composition Triggers Colonic Fungal Community Shifts in a Pig Model.
33712429	11	31	theme	DCHO	2724:2727	arg1	compositions					2708:2719	different compositions	2698:2719	different compositions of DCHO simulated	2698:2737	Here, we show a clear alteration of the colonic fungal community in pigs triggered by different compositions of DCHO simulated by varied concentrations of starch, nonstarch polysaccharides (NSP), and oligosaccharides.
33712429	4	32	theme	polysaccharides	656:670	arg1	level					637:641	level	637:641	level of nonstarch polysaccharides (NSP; 1%, 2%, and 3%)	637:692	Three factors, ratio (2:1, 1:1, and 1:2) of amylose to amylopectin (AM/AP), level of nonstarch polysaccharides (NSP; 1%, 2%, and 3%), and mannan-oligosaccharide (MOS; 400, 800, and 1,200 mg/kg body weight), were considered according to an L9 (34) orthogonal design to form nine diets with different carbohydrate compositions.
33712429	4	32	theme	polysaccharides	656:670	arg1	amylopectin					616:626	amylopectin	616:626	amylopectin (AM/AP)	616:634	Three factors, ratio (2:1, 1:1, and 1:2) of amylose to amylopectin (AM/AP), level of nonstarch polysaccharides (NSP; 1%, 2%, and 3%), and mannan-oligosaccharide (MOS; 400, 800, and 1,200 mg/kg body weight), were considered according to an L9 (34) orthogonal design to form nine diets with different carbohydrate compositions.
33712429	4	32	theme	polysaccharides	656:670	arg1	AM/AP					629:633	AM/AP	629:633	AM/AP	629:633	Three factors, ratio (2:1, 1:1, and 1:2) of amylose to amylopectin (AM/AP), level of nonstarch polysaccharides (NSP; 1%, 2%, and 3%), and mannan-oligosaccharide (MOS; 400, 800, and 1,200 mg/kg body weight), were considered according to an L9 (34) orthogonal design to form nine diets with different carbohydrate compositions.
33712429	4	32	theme	polysaccharides	656:670	arg1	mannan-oligosaccharide					699:720	mannan-oligosaccharide	699:720	mannan-oligosaccharide (MOS; 400, 800, and 1,200 mg/kg body weight)	699:765	Three factors, ratio (2:1, 1:1, and 1:2) of amylose to amylopectin (AM/AP), level of nonstarch polysaccharides (NSP; 1%, 2%, and 3%), and mannan-oligosaccharide (MOS; 400, 800, and 1,200 mg/kg body weight), were considered according to an L9 (34) orthogonal design to form nine diets with different carbohydrate compositions.
33712429	8	33	theme	close	2143:2147	arg1	correlation					2149:2159	a close correlation	2141:2159	a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300)	2141:2401	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	8	34	theme	fatty	2200:2204	arg1	acids					2206:2210	short-chain fatty acids	2188:2210	short-chain fatty acids	2188:2210	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	11	35	theme	colonic	2652:2658	arg1	community					2667:2675	the colonic fungal community	2648:2675	the colonic fungal community	2648:2675	Here, we show a clear alteration of the colonic fungal community in pigs triggered by different compositions of DCHO simulated by varied concentrations of starch, nonstarch polysaccharides (NSP), and oligosaccharides.
33712429	7	36	from	community	1621:1629	arg1	colon					1638:1642	the colon	1634:1642	the colon	1634:1642	Our study provides direct evidence for the relationship between the composition of DCHO and the fungal community in the colon of pigs, which is helpful to understand the function of gut microorganisms in pigs.IMPORTANCE Although fungi are a large group of microorganisms along with bacteria and archaea in the gut of monogastric animals, the nutritional significance of fungi has been ignored for a long time.
33712429	7	36	from	community	1621:1629	arg1	helpful					1662:1668	helpful	1662:1668	helpful	1662:1668	Our study provides direct evidence for the relationship between the composition of DCHO and the fungal community in the colon of pigs, which is helpful to understand the function of gut microorganisms in pigs.IMPORTANCE Although fungi are a large group of microorganisms along with bacteria and archaea in the gut of monogastric animals, the nutritional significance of fungi has been ignored for a long time.
33712429	5	37	theme	NSP	1098:1100	arg1	order					1073:1077	the order	1069:1077	the order of MOS, AM/AP, and NSP	1069:1100	Sequencing based on an Illumina HiSeq 2500 platform targeting the internal transcribed spacer 1 region showed that the fungal community in the colon of the pigs responded to DCHO in the order of MOS, AM/AP, and NSP.
33712429	8	38	from	metabolites	2232:2242	arg1	hindgut					2268:2274	the hindgut	2264:2274	the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300)	2264:2401	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	6	39	theme	large	1105:1109	arg1	part					1111:1114	A large part	1103:1114	A large part of some low-abundance fungal genera	1103:1150	A large part of some low-abundance fungal genera correlated with the composition of DCHO, represented by Saccharomycopsis, Mrakia, Wallemia, Cantharellus, Eurotium, Solicoccozyma, and Penicillium, were also associated with the concentration of glucose and fructose, as well as the activity of β-d-glucosidase in the colonic digesta, suggesting a role of these fungi in the degradation of DCHO in the colon of pigs.
33712429	0	40	theme	Fungi	43:47	arg1	Significance					16:27	The Nutritional Significance	0:27	The Nutritional Significance of Intestinal Fungi	0:47	The Nutritional Significance of Intestinal Fungi: Alteration of Dietary Carbohydrate Composition Triggers Colonic Fungal Community Shifts in a Pig Model.
33712429	2	41	theme	intestinal	415:424	arg1	fungi					426:430	intestinal fungi	415:430	intestinal fungi	415:430	A large number of studies have shown that dietary carbohydrates (DCHO) are related to the bacterial community in the gut, but their relationship with the composition of intestinal fungi is still unknown.
33712429	9	42	theme	DCHO	2516:2519	arg1	utilization					2501:2511	the utilization	2497:2511	the utilization of DCHO	2497:2519	These groundbreaking findings indicate a potential relationship between intestinal fungi and the utilization of DCHO.
33712429	9	42	theme	DCHO	2516:2519	arg1	relationship					2455:2466	a potential relationship	2443:2466	a potential relationship between intestinal fungi	2443:2491	These groundbreaking findings indicate a potential relationship between intestinal fungi and the utilization of DCHO.
33712429	7	43	theme	long	1917:1920	arg1	time					1922:1925	a long time	1915:1925	a long time	1915:1925	Our study provides direct evidence for the relationship between the composition of DCHO and the fungal community in the colon of pigs, which is helpful to understand the function of gut microorganisms in pigs.IMPORTANCE Although fungi are a large group of microorganisms along with bacteria and archaea in the gut of monogastric animals, the nutritional significance of fungi has been ignored for a long time.
33712429	8	44	theme	short-chain	2188:2198	arg1	acids					2206:2210	short-chain fatty acids	2188:2210	short-chain fatty acids	2188:2210	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	10	45	from	changes	2595:2601	arg1	DCHO					2606:2609	DCHO	2606:2609	DCHO	2606:2609	However, no evidence directly proves the response of intestinal fungi to changes in DCHO.
33712429	6	46	theme	DCHO	1187:1190	arg1	composition					1172:1182	the composition	1168:1182	the composition of DCHO, represented by Saccharomycopsis, Mrakia, Wallemia, Cantharellus, Eurotium, Solicoccozyma, and Penicillium,	1168:1298	A large part of some low-abundance fungal genera correlated with the composition of DCHO, represented by Saccharomycopsis, Mrakia, Wallemia, Cantharellus, Eurotium, Solicoccozyma, and Penicillium, were also associated with the concentration of glucose and fructose, as well as the activity of β-d-glucosidase in the colonic digesta, suggesting a role of these fungi in the degradation of DCHO in the colon of pigs.
33712429	7	47	dep	archaea	1813:1819	arg1	ignored					1903:1909	ignored	1903:1909	has been ignored for a long time	1894:1925	Our study provides direct evidence for the relationship between the composition of DCHO and the fungal community in the colon of pigs, which is helpful to understand the function of gut microorganisms in pigs.IMPORTANCE Although fungi are a large group of microorganisms along with bacteria and archaea in the gut of monogastric animals, the nutritional significance of fungi has been ignored for a long time.
33712429	8	48	theme	fungal	1969:1974	arg1	community					1976:1984	a distinct fungal community	1958:1984	a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507)	1958:2135	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	11	49	theme	community	2667:2675	arg1	alteration					2634:2643	a clear alteration	2626:2643	a clear alteration of the colonic fungal community in pigs triggered by different compositions of DCHO simulated by varied concentrations of starch, nonstarch polysaccharides (NSP), and oligosaccharides	2626:2827	Here, we show a clear alteration of the colonic fungal community in pigs triggered by different compositions of DCHO simulated by varied concentrations of starch, nonstarch polysaccharides (NSP), and oligosaccharides.
33712429	8	50	from	carbohydrates	2247:2259	arg1	hindgut					2268:2274	the hindgut	2264:2274	the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300)	2264:2401	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	0	51	theme	Intestinal	32:41	arg1	Fungi					43:47	Intestinal Fungi	32:47	Intestinal Fungi	32:47	The Nutritional Significance of Intestinal Fungi: Alteration of Dietary Carbohydrate Composition Triggers Colonic Fungal Community Shifts in a Pig Model.
33712429	10	52	theme	fungi	2586:2590	arg1	response					2563:2570	the response	2559:2570	the response of intestinal fungi to changes in DCHO	2559:2609	However, no evidence directly proves the response of intestinal fungi to changes in DCHO.
33712429	5	53	theme	transcribed	962:972	arg1	spacer					974:979	the internal transcribed spacer 1	949:981	the internal transcribed spacer 1 region	949:988	Sequencing based on an Illumina HiSeq 2500 platform targeting the internal transcribed spacer 1 region showed that the fungal community in the colon of the pigs responded to DCHO in the order of MOS, AM/AP, and NSP.
33712429	7	54	theme	DCHO	1601:1604	arg1	composition					1586:1596	the composition	1582:1596	the composition of DCHO	1582:1604	Our study provides direct evidence for the relationship between the composition of DCHO and the fungal community in the colon of pigs, which is helpful to understand the function of gut microorganisms in pigs.IMPORTANCE Although fungi are a large group of microorganisms along with bacteria and archaea in the gut of monogastric animals, the nutritional significance of fungi has been ignored for a long time.
33712429	7	54	theme	DCHO	1601:1604	arg1	community					1621:1629	the fungal community	1610:1629	the fungal community in the colon of pigs, which is helpful to understand the function of gut microorganisms in pigs.IMPORTANCE Although fungi are a large group of microorganisms along with bacteria and archaea in the gut of monogastric animals, the nutritional significance of fungi has been ignored for a long time	1610:1925	Our study provides direct evidence for the relationship between the composition of DCHO and the fungal community in the colon of pigs, which is helpful to understand the function of gut microorganisms in pigs.IMPORTANCE Although fungi are a large group of microorganisms along with bacteria and archaea in the gut of monogastric animals, the nutritional significance of fungi has been ignored for a long time.
33712429	6	55	theme	DCHO	1491:1494	arg1	degradation					1476:1486	the degradation	1472:1486	the degradation of DCHO in the colon of pigs	1472:1515	A large part of some low-abundance fungal genera correlated with the composition of DCHO, represented by Saccharomycopsis, Mrakia, Wallemia, Cantharellus, Eurotium, Solicoccozyma, and Penicillium, were also associated with the concentration of glucose and fructose, as well as the activity of β-d-glucosidase in the colonic digesta, suggesting a role of these fungi in the degradation of DCHO in the colon of pigs.
33712429	7	56	theme	fungi	1888:1892	arg1	significance					1872:1883	the nutritional significance	1856:1883	the nutritional significance of fungi	1856:1892	Our study provides direct evidence for the relationship between the composition of DCHO and the fungal community in the colon of pigs, which is helpful to understand the function of gut microorganisms in pigs.IMPORTANCE Although fungi are a large group of microorganisms along with bacteria and archaea in the gut of monogastric animals, the nutritional significance of fungi has been ignored for a long time.
33712429	8	57	theme	microbial	2222:2230	arg1	species					2176:2182	fungal species	2169:2182	fungal species	2169:2182	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	8	57	theme	microbial	2222:2230	arg1	metabolites					2232:2242	the main microbial metabolites	2213:2242	the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300)	2213:2401	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	8	58	theme	grazing	2000:2006	arg1	He					2048:2049	J. Li, D. Chen, B. Yu, J. He	2022:2049	J. Li, D. Chen, B. Yu, J. He	2022:2049	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	8	58	theme	grazing	2000:2006	arg1	pigs					2016:2019	grazing Tibetan pigs	2000:2019	grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507)	2000:2135	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	7	59	theme	direct	1537:1542	arg1	evidence					1544:1551	direct evidence	1537:1551	direct evidence for the relationship between the composition of DCHO and the fungal community in the colon of pigs, which is helpful to understand the function of gut microorganisms in pigs.IMPORTANCE Although fungi are a large group of microorganisms along with bacteria and archaea in the gut of monogastric animals, the nutritional significance of fungi has been ignored for a long time	1537:1925	Our study provides direct evidence for the relationship between the composition of DCHO and the fungal community in the colon of pigs, which is helpful to understand the function of gut microorganisms in pigs.IMPORTANCE Although fungi are a large group of microorganisms along with bacteria and archaea in the gut of monogastric animals, the nutritional significance of fungi has been ignored for a long time.
33712429	8	60	from	community	1976:1984	arg1	gut					1993:1995	the gut	1989:1995	the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507)	1989:2135	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	8	61	theme	pigs	2016:2019	arg1	gut					1993:1995	the gut	1989:1995	the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507)	1989:2135	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	6	62	theme	fungal	1138:1143	arg1	genera					1145:1150	some low-abundance fungal genera	1119:1150	some low-abundance fungal genera	1119:1150	A large part of some low-abundance fungal genera correlated with the composition of DCHO, represented by Saccharomycopsis, Mrakia, Wallemia, Cantharellus, Eurotium, Solicoccozyma, and Penicillium, were also associated with the concentration of glucose and fructose, as well as the activity of β-d-glucosidase in the colonic digesta, suggesting a role of these fungi in the degradation of DCHO in the colon of pigs.
33712429	11	63	theme	starch	2767:2772	arg1	concentrations					2749:2762	varied concentrations	2742:2762	varied concentrations of starch, nonstarch polysaccharides (NSP), and oligosaccharides	2742:2827	Here, we show a clear alteration of the colonic fungal community in pigs triggered by different compositions of DCHO simulated by varied concentrations of starch, nonstarch polysaccharides (NSP), and oligosaccharides.
33712429	4	64	theme	different	850:858	arg1	compositions					873:884	different carbohydrate compositions	850:884	different carbohydrate compositions	850:884	Three factors, ratio (2:1, 1:1, and 1:2) of amylose to amylopectin (AM/AP), level of nonstarch polysaccharides (NSP; 1%, 2%, and 3%), and mannan-oligosaccharide (MOS; 400, 800, and 1,200 mg/kg body weight), were considered according to an L9 (34) orthogonal design to form nine diets with different carbohydrate compositions.
33712429	3	65	theme	fungal	494:499	arg1	community					501:509	the colonic fungal community	482:509	the colonic fungal community	482:509	Here, we report the response of the colonic fungal community to different compositions of DCHO in a pig model.
33712429	7	66	from	function	1688:1695	arg1	pigs.IMPORTANCE					1722:1736	pigs.IMPORTANCE	1722:1736	pigs.IMPORTANCE	1722:1736	Our study provides direct evidence for the relationship between the composition of DCHO and the fungal community in the colon of pigs, which is helpful to understand the function of gut microorganisms in pigs.IMPORTANCE Although fungi are a large group of microorganisms along with bacteria and archaea in the gut of monogastric animals, the nutritional significance of fungi has been ignored for a long time.
33712429	11	67	theme	oligosaccharides	2812:2827	arg1	concentrations					2749:2762	varied concentrations	2742:2762	varied concentrations of starch, nonstarch polysaccharides (NSP), and oligosaccharides	2742:2827	Here, we show a clear alteration of the colonic fungal community in pigs triggered by different compositions of DCHO simulated by varied concentrations of starch, nonstarch polysaccharides (NSP), and oligosaccharides.
33712429	9	68	theme	groundbreaking	2410:2423	arg1	findings					2425:2432	These groundbreaking findings	2404:2432	These groundbreaking findings	2404:2432	These groundbreaking findings indicate a potential relationship between intestinal fungi and the utilization of DCHO.
33712429	8	69	theme	pigs	2279:2282	arg1	hindgut					2268:2274	the hindgut	2264:2274	the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300)	2264:2401	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	2	70	theme	studies	264:270	arg1	number					254:259	A large number	246:259	A large number of studies	246:270	A large number of studies have shown that dietary carbohydrates (DCHO) are related to the bacterial community in the gut, but their relationship with the composition of intestinal fungi is still unknown.
33712429	7	71	theme	nutritional	1860:1870	arg1	significance					1872:1883	the nutritional significance	1856:1883	the nutritional significance of fungi	1856:1892	Our study provides direct evidence for the relationship between the composition of DCHO and the fungal community in the colon of pigs, which is helpful to understand the function of gut microorganisms in pigs.IMPORTANCE Although fungi are a large group of microorganisms along with bacteria and archaea in the gut of monogastric animals, the nutritional significance of fungi has been ignored for a long time.
33712429	6	72	theme	low-abundance	1124:1136	arg1	genera					1145:1150	some low-abundance fungal genera	1119:1150	some low-abundance fungal genera	1119:1150	A large part of some low-abundance fungal genera correlated with the composition of DCHO, represented by Saccharomycopsis, Mrakia, Wallemia, Cantharellus, Eurotium, Solicoccozyma, and Penicillium, were also associated with the concentration of glucose and fructose, as well as the activity of β-d-glucosidase in the colonic digesta, suggesting a role of these fungi in the degradation of DCHO in the colon of pigs.
33712429	8	73	from	correlation	2149:2159	arg1	gut					1993:1995	the gut	1989:1995	the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507)	1989:2135	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	1	74	theme	important	187:195	arg1	source					204:209	the most important energy source	178:209	the most important energy source in the diet of humans and animals	178:243	Carbohydrates represent the most important energy source in the diet of humans and animals.
33712429	7	75	theme	microorganisms	1704:1717	arg1	function					1688:1695	the function	1684:1695	the function of gut microorganisms in pigs.IMPORTANCE	1684:1736	Our study provides direct evidence for the relationship between the composition of DCHO and the fungal community in the colon of pigs, which is helpful to understand the function of gut microorganisms in pigs.IMPORTANCE Although fungi are a large group of microorganisms along with bacteria and archaea in the gut of monogastric animals, the nutritional significance of fungi has been ignored for a long time.
33712429	2	76	from	community	346:354	arg1	gut					363:365	the gut	359:365	the gut	359:365	A large number of studies have shown that dietary carbohydrates (DCHO) are related to the bacterial community in the gut, but their relationship with the composition of intestinal fungi is still unknown.
33712429	9	77	theme	potential	2445:2453	arg1	relationship					2455:2466	a potential relationship	2443:2466	a potential relationship between intestinal fungi	2443:2491	These groundbreaking findings indicate a potential relationship between intestinal fungi and the utilization of DCHO.
33712429	5	78	from	community	1013:1021	arg1	colon					1030:1034	the colon	1026:1034	the colon of the pigs	1026:1046	Sequencing based on an Illumina HiSeq 2500 platform targeting the internal transcribed spacer 1 region showed that the fungal community in the colon of the pigs responded to DCHO in the order of MOS, AM/AP, and NSP.
33712429	2	79	theme	dietary	288:294	arg1	DCHO					311:314	DCHO	311:314	DCHO	311:314	A large number of studies have shown that dietary carbohydrates (DCHO) are related to the bacterial community in the gut, but their relationship with the composition of intestinal fungi is still unknown.
33712429	2	79	theme	dietary	288:294	arg1	carbohydrates					296:308	dietary carbohydrates	288:308	dietary carbohydrates (DCHO)	288:315	A large number of studies have shown that dietary carbohydrates (DCHO) are related to the bacterial community in the gut, but their relationship with the composition of intestinal fungi is still unknown.
33712429	7	80	theme	monogastric	1835:1845	arg1	animals					1847:1853	monogastric animals	1835:1853	monogastric animals	1835:1853	Our study provides direct evidence for the relationship between the composition of DCHO and the fungal community in the colon of pigs, which is helpful to understand the function of gut microorganisms in pigs.IMPORTANCE Although fungi are a large group of microorganisms along with bacteria and archaea in the gut of monogastric animals, the nutritional significance of fungi has been ignored for a long time.
33712429	0	81	theme	Pig	143:145	arg1	Model					147:151	a Pig Model	141:151	a Pig Model	141:151	The Nutritional Significance of Intestinal Fungi: Alteration of Dietary Carbohydrate Composition Triggers Colonic Fungal Community Shifts in a Pig Model.
33712429	7	82	dep	animals	1847:1853	arg1	the					1824:1826	the	1824:1826	the	1824:1826	Our study provides direct evidence for the relationship between the composition of DCHO and the fungal community in the colon of pigs, which is helpful to understand the function of gut microorganisms in pigs.IMPORTANCE Although fungi are a large group of microorganisms along with bacteria and archaea in the gut of monogastric animals, the nutritional significance of fungi has been ignored for a long time.
33712429	7	82	dep	animals	1847:1853	arg1	gut					1828:1830	gut	1828:1830	gut	1828:1830	Our study provides direct evidence for the relationship between the composition of DCHO and the fungal community in the colon of pigs, which is helpful to understand the function of gut microorganisms in pigs.IMPORTANCE Although fungi are a large group of microorganisms along with bacteria and archaea in the gut of monogastric animals, the nutritional significance of fungi has been ignored for a long time.
33712429	6	83	from	activity	1384:1391	arg1	digesta					1427:1433	the colonic digesta	1415:1433	the colonic digesta	1415:1433	A large part of some low-abundance fungal genera correlated with the composition of DCHO, represented by Saccharomycopsis, Mrakia, Wallemia, Cantharellus, Eurotium, Solicoccozyma, and Penicillium, were also associated with the concentration of glucose and fructose, as well as the activity of β-d-glucosidase in the colonic digesta, suggesting a role of these fungi in the degradation of DCHO in the colon of pigs.
33712429	4	84	theme	1,200 mg/kg	742:752	arg1	weight					759:764	1,200 mg/kg body weight	742:764	1,200 mg/kg body weight	742:764	Three factors, ratio (2:1, 1:1, and 1:2) of amylose to amylopectin (AM/AP), level of nonstarch polysaccharides (NSP; 1%, 2%, and 3%), and mannan-oligosaccharide (MOS; 400, 800, and 1,200 mg/kg body weight), were considered according to an L9 (34) orthogonal design to form nine diets with different carbohydrate compositions.
33712429	3	85	theme	DCHO	540:543	arg1	compositions					524:535	different compositions	514:535	different compositions of DCHO in a pig model	514:558	Here, we report the response of the colonic fungal community to different compositions of DCHO in a pig model.
33712429	6	86	theme	glucose	1347:1353	arg1	concentration					1330:1342	the concentration	1326:1342	the concentration of glucose and fructose	1326:1366	A large part of some low-abundance fungal genera correlated with the composition of DCHO, represented by Saccharomycopsis, Mrakia, Wallemia, Cantharellus, Eurotium, Solicoccozyma, and Penicillium, were also associated with the concentration of glucose and fructose, as well as the activity of β-d-glucosidase in the colonic digesta, suggesting a role of these fungi in the degradation of DCHO in the colon of pigs.
33712429	6	87	from	DCHO	1491:1494	arg1	colon					1503:1507	the colon	1499:1507	the colon of pigs	1499:1515	A large part of some low-abundance fungal genera correlated with the composition of DCHO, represented by Saccharomycopsis, Mrakia, Wallemia, Cantharellus, Eurotium, Solicoccozyma, and Penicillium, were also associated with the concentration of glucose and fructose, as well as the activity of β-d-glucosidase in the colonic digesta, suggesting a role of these fungi in the degradation of DCHO in the colon of pigs.
33712429	6	88	theme	colonic	1419:1425	arg1	digesta					1427:1433	the colonic digesta	1415:1433	the colonic digesta	1415:1433	A large part of some low-abundance fungal genera correlated with the composition of DCHO, represented by Saccharomycopsis, Mrakia, Wallemia, Cantharellus, Eurotium, Solicoccozyma, and Penicillium, were also associated with the concentration of glucose and fructose, as well as the activity of β-d-glucosidase in the colonic digesta, suggesting a role of these fungi in the degradation of DCHO in the colon of pigs.
33712429	8	89	theme	previous	1932:1939	arg1	studies					1941:1947	Our previous studies	1928:1947	Our previous studies	1928:1947	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	3	90	from	compositions	524:535	arg1	model					554:558	a pig model	548:558	a pig model	548:558	Here, we report the response of the colonic fungal community to different compositions of DCHO in a pig model.
33712429	0	91	theme	Dietary	64:70	arg1	Composition					85:95	Dietary Carbohydrate Composition	64:95	Dietary Carbohydrate Composition	64:95	The Nutritional Significance of Intestinal Fungi: Alteration of Dietary Carbohydrate Composition Triggers Colonic Fungal Community Shifts in a Pig Model.
33712429	12	92	theme	nutrients	2920:2928	arg1	utilization					2905:2915	the utilization	2901:2915	the utilization of nutrients in monogastric animals	2901:2951	Our results highlight the potential involvement of intestinal fungi in the utilization of nutrients in monogastric animals.
33712429	4	93	dep	NSP	673:675	arg1	%					679:679	1%	678:679	1%	678:679	Three factors, ratio (2:1, 1:1, and 1:2) of amylose to amylopectin (AM/AP), level of nonstarch polysaccharides (NSP; 1%, 2%, and 3%), and mannan-oligosaccharide (MOS; 400, 800, and 1,200 mg/kg body weight), were considered according to an L9 (34) orthogonal design to form nine diets with different carbohydrate compositions.
33712429	4	93	dep	NSP	673:675	arg1	%					683:683	2%	682:683	2%	682:683	Three factors, ratio (2:1, 1:1, and 1:2) of amylose to amylopectin (AM/AP), level of nonstarch polysaccharides (NSP; 1%, 2%, and 3%), and mannan-oligosaccharide (MOS; 400, 800, and 1,200 mg/kg body weight), were considered according to an L9 (34) orthogonal design to form nine diets with different carbohydrate compositions.
33712429	4	93	dep	NSP	673:675	arg1	%					691:691	3%	690:691	3%	690:691	Three factors, ratio (2:1, 1:1, and 1:2) of amylose to amylopectin (AM/AP), level of nonstarch polysaccharides (NSP; 1%, 2%, and 3%), and mannan-oligosaccharide (MOS; 400, 800, and 1,200 mg/kg body weight), were considered according to an L9 (34) orthogonal design to form nine diets with different carbohydrate compositions.
33712429	11	94	theme	different	2698:2706	arg1	compositions					2708:2719	different compositions	2698:2719	different compositions of DCHO simulated	2698:2737	Here, we show a clear alteration of the colonic fungal community in pigs triggered by different compositions of DCHO simulated by varied concentrations of starch, nonstarch polysaccharides (NSP), and oligosaccharides.
33712429	8	95	theme	Biotechnol	2067:2076	arg1	He					2048:2049	J. Li, D. Chen, B. Yu, J. He	2022:2049	J. Li, D. Chen, B. Yu, J. He	2022:2049	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	8	95	theme	Biotechnol	2067:2076	arg1	13:509-521					2078:2087	Microb Biotechnol 13:509-521	2060:2087	Microb Biotechnol 13:509-521	2060:2087	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	5	96	theme	pigs	1043:1046	arg1	colon					1030:1034	the colon	1026:1034	the colon of the pigs	1026:1046	Sequencing based on an Illumina HiSeq 2500 platform targeting the internal transcribed spacer 1 region showed that the fungal community in the colon of the pigs responded to DCHO in the order of MOS, AM/AP, and NSP.
33712429	0	97	theme	Composition	85:95	arg1	Alteration					50:59	Alteration	50:59	Alteration of Dietary Carbohydrate Composition	50:95	The Nutritional Significance of Intestinal Fungi: Alteration of Dietary Carbohydrate Composition Triggers Colonic Fungal Community Shifts in a Pig Model.
33712429	4	98	dep	polysaccharides	656:670	arg1	NSP					673:675	NSP	673:675	NSP	673:675	Three factors, ratio (2:1, 1:1, and 1:2) of amylose to amylopectin (AM/AP), level of nonstarch polysaccharides (NSP; 1%, 2%, and 3%), and mannan-oligosaccharide (MOS; 400, 800, and 1,200 mg/kg body weight), were considered according to an L9 (34) orthogonal design to form nine diets with different carbohydrate compositions.
33712429	11	99	theme	varied	2742:2747	arg1	concentrations					2749:2762	varied concentrations	2742:2762	varied concentrations of starch, nonstarch polysaccharides (NSP), and oligosaccharides	2742:2827	Here, we show a clear alteration of the colonic fungal community in pigs triggered by different compositions of DCHO simulated by varied concentrations of starch, nonstarch polysaccharides (NSP), and oligosaccharides.
33712429	6	100	from	degradation	1476:1486	arg1	colon					1503:1507	the colon	1499:1507	the colon of pigs	1499:1515	A large part of some low-abundance fungal genera correlated with the composition of DCHO, represented by Saccharomycopsis, Mrakia, Wallemia, Cantharellus, Eurotium, Solicoccozyma, and Penicillium, were also associated with the concentration of glucose and fructose, as well as the activity of β-d-glucosidase in the colonic digesta, suggesting a role of these fungi in the degradation of DCHO in the colon of pigs.
33712429	7	101	theme	microorganisms	1774:1787	arg1	fungi					1747:1751	fungi	1747:1751	fungi	1747:1751	Our study provides direct evidence for the relationship between the composition of DCHO and the fungal community in the colon of pigs, which is helpful to understand the function of gut microorganisms in pigs.IMPORTANCE Although fungi are a large group of microorganisms along with bacteria and archaea in the gut of monogastric animals, the nutritional significance of fungi has been ignored for a long time.
33712429	7	101	theme	microorganisms	1774:1787	arg1	microorganisms					1774:1787	microorganisms	1774:1787	microorganisms	1774:1787	Our study provides direct evidence for the relationship between the composition of DCHO and the fungal community in the colon of pigs, which is helpful to understand the function of gut microorganisms in pigs.IMPORTANCE Although fungi are a large group of microorganisms along with bacteria and archaea in the gut of monogastric animals, the nutritional significance of fungi has been ignored for a long time.
33712429	7	101	theme	microorganisms	1774:1787	arg1	group					1765:1769	a large group	1757:1769	a large group of microorganisms	1757:1787	Our study provides direct evidence for the relationship between the composition of DCHO and the fungal community in the colon of pigs, which is helpful to understand the function of gut microorganisms in pigs.IMPORTANCE Although fungi are a large group of microorganisms along with bacteria and archaea in the gut of monogastric animals, the nutritional significance of fungi has been ignored for a long time.
33712429	0	102	theme	Colonic	106:112	arg1	Shifts					131:136	Colonic Fungal Community Shifts	106:136	Colonic Fungal Community Shifts	106:136	The Nutritional Significance of Intestinal Fungi: Alteration of Dietary Carbohydrate Composition Triggers Colonic Fungal Community Shifts in a Pig Model.
33712429	7	103	theme	pigs	1647:1650	arg1	colon					1638:1642	the colon	1634:1642	the colon	1634:1642	Our study provides direct evidence for the relationship between the composition of DCHO and the fungal community in the colon of pigs, which is helpful to understand the function of gut microorganisms in pigs.IMPORTANCE Although fungi are a large group of microorganisms along with bacteria and archaea in the gut of monogastric animals, the nutritional significance of fungi has been ignored for a long time.
33712429	7	103	theme	pigs	1647:1650	arg1	helpful					1662:1668	helpful	1662:1668	helpful	1662:1668	Our study provides direct evidence for the relationship between the composition of DCHO and the fungal community in the colon of pigs, which is helpful to understand the function of gut microorganisms in pigs.IMPORTANCE Although fungi are a large group of microorganisms along with bacteria and archaea in the gut of monogastric animals, the nutritional significance of fungi has been ignored for a long time.
33712429	11	104	theme	simulated	2729:2737	arg1	DCHO					2724:2727	DCHO simulated	2724:2737	DCHO simulated	2724:2737	Here, we show a clear alteration of the colonic fungal community in pigs triggered by different compositions of DCHO simulated by varied concentrations of starch, nonstarch polysaccharides (NSP), and oligosaccharides.
33712429	6	105	from	role	1449:1452	arg1	degradation					1476:1486	the degradation	1472:1486	the degradation of DCHO in the colon of pigs	1472:1515	A large part of some low-abundance fungal genera correlated with the composition of DCHO, represented by Saccharomycopsis, Mrakia, Wallemia, Cantharellus, Eurotium, Solicoccozyma, and Penicillium, were also associated with the concentration of glucose and fructose, as well as the activity of β-d-glucosidase in the colonic digesta, suggesting a role of these fungi in the degradation of DCHO in the colon of pigs.
33712429	6	106	theme	fungi	1463:1467	arg1	role					1449:1452	a role	1447:1452	a role of these fungi in the degradation of DCHO in the colon of pigs	1447:1515	A large part of some low-abundance fungal genera correlated with the composition of DCHO, represented by Saccharomycopsis, Mrakia, Wallemia, Cantharellus, Eurotium, Solicoccozyma, and Penicillium, were also associated with the concentration of glucose and fructose, as well as the activity of β-d-glucosidase in the colonic digesta, suggesting a role of these fungi in the degradation of DCHO in the colon of pigs.
33712429	4	107	theme	nonstarch	646:654	arg1	polysaccharides					656:670	nonstarch polysaccharides	646:670	nonstarch polysaccharides (NSP; 1%, 2%, and 3%)	646:692	Three factors, ratio (2:1, 1:1, and 1:2) of amylose to amylopectin (AM/AP), level of nonstarch polysaccharides (NSP; 1%, 2%, and 3%), and mannan-oligosaccharide (MOS; 400, 800, and 1,200 mg/kg body weight), were considered according to an L9 (34) orthogonal design to form nine diets with different carbohydrate compositions.
33712429	3	108	from	model	554:558	arg1	compositions					524:535	different compositions	514:535	different compositions of DCHO in a pig model	514:558	Here, we report the response of the colonic fungal community to different compositions of DCHO in a pig model.
33712429	0	109	theme	Community	121:129	arg1	Shifts					131:136	Colonic Fungal Community Shifts	106:136	Colonic Fungal Community Shifts	106:136	The Nutritional Significance of Intestinal Fungi: Alteration of Dietary Carbohydrate Composition Triggers Colonic Fungal Community Shifts in a Pig Model.
33712429	8	110	theme	fungal	2169:2174	arg1	species					2176:2182	fungal species	2169:2182	fungal species	2169:2182	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	8	110	theme	fungal	2169:2174	arg1	metabolites					2232:2242	the main microbial metabolites	2213:2242	the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300)	2213:2401	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	11	111	theme	fungal	2660:2665	arg1	community					2667:2675	the colonic fungal community	2648:2675	the colonic fungal community	2648:2675	Here, we show a clear alteration of the colonic fungal community in pigs triggered by different compositions of DCHO simulated by varied concentrations of starch, nonstarch polysaccharides (NSP), and oligosaccharides.
33712429	0	112	theme	Nutritional	4:14	arg1	Significance					16:27	The Nutritional Significance	0:27	The Nutritional Significance of Intestinal Fungi	0:47	The Nutritional Significance of Intestinal Fungi: Alteration of Dietary Carbohydrate Composition Triggers Colonic Fungal Community Shifts in a Pig Model.
33712429	4	113	with	diets	839:843	arg1	compositions					873:884	different carbohydrate compositions	850:884	different carbohydrate compositions	850:884	Three factors, ratio (2:1, 1:1, and 1:2) of amylose to amylopectin (AM/AP), level of nonstarch polysaccharides (NSP; 1%, 2%, and 3%), and mannan-oligosaccharide (MOS; 400, 800, and 1,200 mg/kg body weight), were considered according to an L9 (34) orthogonal design to form nine diets with different carbohydrate compositions.
33712429	4	114	theme	orthogonal	808:817	arg1	design					819:824	an L9 (34) orthogonal design	797:824	an L9 (34) orthogonal design	797:824	Three factors, ratio (2:1, 1:1, and 1:2) of amylose to amylopectin (AM/AP), level of nonstarch polysaccharides (NSP; 1%, 2%, and 3%), and mannan-oligosaccharide (MOS; 400, 800, and 1,200 mg/kg body weight), were considered according to an L9 (34) orthogonal design to form nine diets with different carbohydrate compositions.
33712429	3	115	from	DCHO	540:543	arg1	model					554:558	a pig model	548:558	a pig model	548:558	Here, we report the response of the colonic fungal community to different compositions of DCHO in a pig model.
33712429	7	116	theme	fungal	1614:1619	arg1	community					1621:1629	the fungal community	1610:1629	the fungal community in the colon of pigs, which is helpful to understand the function of gut microorganisms in pigs.IMPORTANCE Although fungi are a large group of microorganisms along with bacteria and archaea in the gut of monogastric animals, the nutritional significance of fungi has been ignored for a long time	1610:1925	Our study provides direct evidence for the relationship between the composition of DCHO and the fungal community in the colon of pigs, which is helpful to understand the function of gut microorganisms in pigs.IMPORTANCE Although fungi are a large group of microorganisms along with bacteria and archaea in the gut of monogastric animals, the nutritional significance of fungi has been ignored for a long time.
33712429	6	117	theme	fructose	1359:1366	arg1	concentration					1330:1342	the concentration	1326:1342	the concentration of glucose and fructose	1326:1366	A large part of some low-abundance fungal genera correlated with the composition of DCHO, represented by Saccharomycopsis, Mrakia, Wallemia, Cantharellus, Eurotium, Solicoccozyma, and Penicillium, were also associated with the concentration of glucose and fructose, as well as the activity of β-d-glucosidase in the colonic digesta, suggesting a role of these fungi in the degradation of DCHO in the colon of pigs.
33712429	8	118	dep	pigs	2279:2282	arg1	https					2368:2372	https	2368:2372	https	2368:2372	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	8	118	dep	pigs	2279:2282	arg1	Nutr					2344:2347	Nutr	2344:2347	Nutr	2344:2347	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	8	118	dep	pigs	2279:2282	arg1	D.					2300:2301	D.	2300:2301	D.	2300:2301	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	8	118	dep	pigs	2279:2282	arg1	Y.					2292:2293	Y.	2292:2293	Y.	2292:2293	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	8	118	dep	pigs	2279:2282	arg1	B.					2309:2310	B.	2309:2310	B.	2309:2310	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	8	118	dep	pigs	2279:2282	arg1	Yu					2312:2313	Yu	2312:2313	Yu	2312:2313	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	6	119	theme	pigs	1512:1515	arg1	colon					1503:1507	the colon	1499:1507	the colon of pigs	1499:1515	A large part of some low-abundance fungal genera correlated with the composition of DCHO, represented by Saccharomycopsis, Mrakia, Wallemia, Cantharellus, Eurotium, Solicoccozyma, and Penicillium, were also associated with the concentration of glucose and fructose, as well as the activity of β-d-glucosidase in the colonic digesta, suggesting a role of these fungi in the degradation of DCHO in the colon of pigs.
33712429	5	120	theme	AM/AP	1087:1091	arg1	order					1073:1077	the order	1069:1077	the order of MOS, AM/AP, and NSP	1069:1100	Sequencing based on an Illumina HiSeq 2500 platform targeting the internal transcribed spacer 1 region showed that the fungal community in the colon of the pigs responded to DCHO in the order of MOS, AM/AP, and NSP.
33712429	4	121	dep	MOS	723:725	arg1	weight					759:764	1,200 mg/kg body weight	742:764	1,200 mg/kg body weight	742:764	Three factors, ratio (2:1, 1:1, and 1:2) of amylose to amylopectin (AM/AP), level of nonstarch polysaccharides (NSP; 1%, 2%, and 3%), and mannan-oligosaccharide (MOS; 400, 800, and 1,200 mg/kg body weight), were considered according to an L9 (34) orthogonal design to form nine diets with different carbohydrate compositions.
33712429	4	121	dep	MOS	723:725	arg1	800					733:735	800	733:735	800	733:735	Three factors, ratio (2:1, 1:1, and 1:2) of amylose to amylopectin (AM/AP), level of nonstarch polysaccharides (NSP; 1%, 2%, and 3%), and mannan-oligosaccharide (MOS; 400, 800, and 1,200 mg/kg body weight), were considered according to an L9 (34) orthogonal design to form nine diets with different carbohydrate compositions.
33712429	4	121	dep	MOS	723:725	arg1	400					728:730	400	728:730	400	728:730	Three factors, ratio (2:1, 1:1, and 1:2) of amylose to amylopectin (AM/AP), level of nonstarch polysaccharides (NSP; 1%, 2%, and 3%), and mannan-oligosaccharide (MOS; 400, 800, and 1,200 mg/kg body weight), were considered according to an L9 (34) orthogonal design to form nine diets with different carbohydrate compositions.
33712429	12	122	theme	intestinal	2881:2890	arg1	fungi					2892:2896	intestinal fungi	2881:2896	intestinal fungi	2881:2896	Our results highlight the potential involvement of intestinal fungi in the utilization of nutrients in monogastric animals.
33712429	8	123	theme	distinct	1960:1967	arg1	community					1976:1984	a distinct fungal community	1958:1984	a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507)	1958:2135	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	4	124	theme	L9	800:801	arg1	design					819:824	an L9 (34) orthogonal design	797:824	an L9 (34) orthogonal design	797:824	Three factors, ratio (2:1, 1:1, and 1:2) of amylose to amylopectin (AM/AP), level of nonstarch polysaccharides (NSP; 1%, 2%, and 3%), and mannan-oligosaccharide (MOS; 400, 800, and 1,200 mg/kg body weight), were considered according to an L9 (34) orthogonal design to form nine diets with different carbohydrate compositions.
33712429	8	125	theme	main	2217:2220	arg1	species					2176:2182	fungal species	2169:2182	fungal species	2169:2182	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	8	125	theme	main	2217:2220	arg1	metabolites					2232:2242	the main microbial metabolites	2213:2242	the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300)	2213:2401	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	0	126	theme	Fungal	114:119	arg1	Shifts					131:136	Colonic Fungal Community Shifts	106:136	Colonic Fungal Community Shifts	106:136	The Nutritional Significance of Intestinal Fungi: Alteration of Dietary Carbohydrate Composition Triggers Colonic Fungal Community Shifts in a Pig Model.
33712429	4	127	dep	mannan-oligosaccharide	699:720	arg1	MOS					723:725	MOS	723:725	MOS; 400, 800, and 1,200 mg/kg body weight	723:764	Three factors, ratio (2:1, 1:1, and 1:2) of amylose to amylopectin (AM/AP), level of nonstarch polysaccharides (NSP; 1%, 2%, and 3%), and mannan-oligosaccharide (MOS; 400, 800, and 1,200 mg/kg body weight), were considered according to an L9 (34) orthogonal design to form nine diets with different carbohydrate compositions.
33712429	5	128	theme	internal	953:960	arg1	spacer					974:979	the internal transcribed spacer 1	949:981	the internal transcribed spacer 1 region	949:988	Sequencing based on an Illumina HiSeq 2500 platform targeting the internal transcribed spacer 1 region showed that the fungal community in the colon of the pigs responded to DCHO in the order of MOS, AM/AP, and NSP.
33712429	8	129	dep	Yu	2312:2313	arg1	//doi.org/10.1111/jpn.13300					2374:2400	//doi.org/10.1111/jpn.13300	2374:2400	//doi.org/10.1111/jpn.13300	2374:2400	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	4	130	dep	ratio	576:580	arg1	1:1					588:590	1:1	588:590	1:1	588:590	Three factors, ratio (2:1, 1:1, and 1:2) of amylose to amylopectin (AM/AP), level of nonstarch polysaccharides (NSP; 1%, 2%, and 3%), and mannan-oligosaccharide (MOS; 400, 800, and 1,200 mg/kg body weight), were considered according to an L9 (34) orthogonal design to form nine diets with different carbohydrate compositions.
33712429	4	130	dep	ratio	576:580	arg1	1:2					597:599	1:2	597:599	1:2	597:599	Three factors, ratio (2:1, 1:1, and 1:2) of amylose to amylopectin (AM/AP), level of nonstarch polysaccharides (NSP; 1%, 2%, and 3%), and mannan-oligosaccharide (MOS; 400, 800, and 1,200 mg/kg body weight), were considered according to an L9 (34) orthogonal design to form nine diets with different carbohydrate compositions.
33712429	4	130	dep	ratio	576:580	arg1	2:1					583:585	2:1	583:585	2:1	583:585	Three factors, ratio (2:1, 1:1, and 1:2) of amylose to amylopectin (AM/AP), level of nonstarch polysaccharides (NSP; 1%, 2%, and 3%), and mannan-oligosaccharide (MOS; 400, 800, and 1,200 mg/kg body weight), were considered according to an L9 (34) orthogonal design to form nine diets with different carbohydrate compositions.
33712429	10	131	theme	intestinal	2575:2584	arg1	fungi					2586:2590	intestinal fungi	2575:2590	intestinal fungi	2575:2590	However, no evidence directly proves the response of intestinal fungi to changes in DCHO.
33712429	2	132	theme	fungi	426:430	arg1	composition					400:410	the composition	396:410	the composition of intestinal fungi	396:430	A large number of studies have shown that dietary carbohydrates (DCHO) are related to the bacterial community in the gut, but their relationship with the composition of intestinal fungi is still unknown.
33712429	5	133	theme	spacer	974:979	arg1	region					983:988	the internal transcribed spacer 1 region	949:988	the internal transcribed spacer 1 region	949:988	Sequencing based on an Illumina HiSeq 2500 platform targeting the internal transcribed spacer 1 region showed that the fungal community in the colon of the pigs responded to DCHO in the order of MOS, AM/AP, and NSP.
33712429	4	134	theme	carbohydrate	860:871	arg1	compositions					873:884	different carbohydrate compositions	850:884	different carbohydrate compositions	850:884	Three factors, ratio (2:1, 1:1, and 1:2) of amylose to amylopectin (AM/AP), level of nonstarch polysaccharides (NSP; 1%, 2%, and 3%), and mannan-oligosaccharide (MOS; 400, 800, and 1,200 mg/kg body weight), were considered according to an L9 (34) orthogonal design to form nine diets with different carbohydrate compositions.
33712429	8	135	theme	carbohydrates	2247:2259	arg1	species					2176:2182	fungal species	2169:2182	fungal species	2169:2182	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	8	135	theme	carbohydrates	2247:2259	arg1	metabolites					2232:2242	the main microbial metabolites	2213:2242	the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300)	2213:2401	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	8	136	from	hindgut	2268:2274	arg1	species					2176:2182	fungal species	2169:2182	fungal species	2169:2182	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	8	136	from	hindgut	2268:2274	arg1	metabolites					2232:2242	the main microbial metabolites	2213:2242	the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300)	2213:2401	Our previous studies revealed a distinct fungal community in the gut of grazing Tibetan pigs (J. Li, D. Chen, B. Yu, J. He, et al., Microb Biotechnol 13:509-521, 2020, https://doi.org/10.1111/1751-7915.13507) and a close correlation between fungal species and short-chain fatty acids, the main microbial metabolites of carbohydrates in the hindgut of pigs (J. Li, Y. Luo, D. Chen, B. Yu, et al., J Anim Physiol Anim Nutr 104:616-628, 2020, https://doi.org/10.1111/jpn.13300).
33712429	1	137	theme	humans	226:231	arg1	diet					218:221	the diet	214:221	the diet of humans and animals	214:243	Carbohydrates represent the most important energy source in the diet of humans and animals.
33712429	7	138	theme	large	1759:1763	arg1	fungi					1747:1751	fungi	1747:1751	fungi	1747:1751	Our study provides direct evidence for the relationship between the composition of DCHO and the fungal community in the colon of pigs, which is helpful to understand the function of gut microorganisms in pigs.IMPORTANCE Although fungi are a large group of microorganisms along with bacteria and archaea in the gut of monogastric animals, the nutritional significance of fungi has been ignored for a long time.
33712429	7	138	theme	large	1759:1763	arg1	microorganisms					1774:1787	microorganisms	1774:1787	microorganisms	1774:1787	Our study provides direct evidence for the relationship between the composition of DCHO and the fungal community in the colon of pigs, which is helpful to understand the function of gut microorganisms in pigs.IMPORTANCE Although fungi are a large group of microorganisms along with bacteria and archaea in the gut of monogastric animals, the nutritional significance of fungi has been ignored for a long time.
33712429	7	138	theme	large	1759:1763	arg1	group					1765:1769	a large group	1757:1769	a large group of microorganisms	1757:1787	Our study provides direct evidence for the relationship between the composition of DCHO and the fungal community in the colon of pigs, which is helpful to understand the function of gut microorganisms in pigs.IMPORTANCE Although fungi are a large group of microorganisms along with bacteria and archaea in the gut of monogastric animals, the nutritional significance of fungi has been ignored for a long time.
33712429	2	139	theme	bacterial	336:344	arg1	community					346:354	the bacterial community	332:354	the bacterial community in the gut	332:365	A large number of studies have shown that dietary carbohydrates (DCHO) are related to the bacterial community in the gut, but their relationship with the composition of intestinal fungi is still unknown.
33712429	12	140	theme	fungi	2892:2896	arg1	involvement					2866:2876	the potential involvement	2852:2876	the potential involvement of intestinal fungi in the utilization of nutrients in monogastric animals	2852:2951	Our results highlight the potential involvement of intestinal fungi in the utilization of nutrients in monogastric animals.
33712429	1	141	theme	animals	237:243	arg1	diet					218:221	the diet	214:221	the diet of humans and animals	214:243	Carbohydrates represent the most important energy source in the diet of humans and animals.
32338160	0	0	theme	secretory	82:90	arg1	cluster					100:106	secretory vesicle cluster	82:106	secretory vesicle cluster	82:106	Sucrose starvation induces the degradation of proteins in trans-Golgi network and secretory vesicle cluster in tobacco BY-2 cells.
32338160	5	1	theme	starvation	746:755	arg1	condition					757:765	the same starvation condition	737:765	the same starvation condition	737:765	Under the same starvation condition, protein secretion was not arrested but pectin deposition to cell wall was suppressed.
32338160	2	2	theme	sucrose-transporter	327:345	arg1	ortholog					347:354	a tobacco sucrose-transporter ortholog	317:354	a tobacco sucrose-transporter ortholog	317:354	We found that SUT2, a tobacco sucrose-transporter ortholog and was localized in the TGN, decreased significantly under a sucrose-starvation condition.
32338160	2	2	theme	sucrose-transporter	327:345	arg1	SUT2					311:314	SUT2	311:314	SUT2	311:314	We found that SUT2, a tobacco sucrose-transporter ortholog and was localized in the TGN, decreased significantly under a sucrose-starvation condition.
32338160	4	3	from	SVC	645:647	arg1	protein					609:615	the SCAMP2-RFP fusion protein	587:615	the SCAMP2-RFP fusion protein	587:615	Similarly, the SCAMP2-RFP fusion protein, which is localized in TGN, SVC, and plasma membrane (PM), was distributed solely in the PM under the starvation.
32338160	4	3	from	SVC	645:647	arg1	localized					627:635	localized	627:635	localized	627:635	Similarly, the SCAMP2-RFP fusion protein, which is localized in TGN, SVC, and plasma membrane (PM), was distributed solely in the PM under the starvation.
32338160	8	4	theme	independent	1218:1228	arg1	TGN					1230:1232	Golgi-released independent TGN	1203:1232	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	8	5	theme	membrane	1389:1396	arg1	protein					1398:1404	secretory carrier membrane protein 2	1371:1406	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	7	6	theme	SVC	1029:1031	arg1	involvement					1014:1024	the involvement	1010:1024	our findings as well as the involvement of SVC in pectin secretion	986:1051	Furthermore, our findings as well as the involvement of SVC in pectin secretion suggested that synthesis and transport of pectin are regulated by the level of extracellular sugars.
32338160	7	6	theme	SVC	1029:1031	arg1	findings					990:997	our findings	986:997	our findings as well as the involvement of SVC in pectin secretion	986:1051	Furthermore, our findings as well as the involvement of SVC in pectin secretion suggested that synthesis and transport of pectin are regulated by the level of extracellular sugars.
32338160	2	7	theme	tobacco	319:325	arg1	ortholog					347:354	a tobacco sucrose-transporter ortholog	317:354	a tobacco sucrose-transporter ortholog	317:354	We found that SUT2, a tobacco sucrose-transporter ortholog and was localized in the TGN, decreased significantly under a sucrose-starvation condition.
32338160	2	7	theme	tobacco	319:325	arg1	SUT2					311:314	SUT2	311:314	SUT2	311:314	We found that SUT2, a tobacco sucrose-transporter ortholog and was localized in the TGN, decreased significantly under a sucrose-starvation condition.
32338160	1	8	theme	subsequent	240:249	arg1	compartment					251:261	subsequent compartment	240:261	subsequent compartment	240:261	Endomembrane transport system begins at the endoplasmic reticulum (ER), continues to the Golgi apparatus and subsequent compartment called trans-Golgi network (TGN).
32338160	8	9	theme	carrier	1381:1387	arg1	protein					1398:1404	secretory carrier membrane protein 2	1371:1406	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	3	10	theme	TGN	498:500	arg1	cluster					524:530	the TGN and secretory vesicle cluster	494:530	the TGN and secretory vesicle cluster (SVC)	494:536	The tobacco SNARE protein SYP41, localized in the TGN and secretory vesicle cluster (SVC), also decreased under the starvation.
32338160	3	10	theme	TGN	498:500	arg1	SVC					533:535	SVC	533:535	SVC	533:535	The tobacco SNARE protein SYP41, localized in the TGN and secretory vesicle cluster (SVC), also decreased under the starvation.
32338160	4	11	from	TGN	640:642	arg1	protein					609:615	the SCAMP2-RFP fusion protein	587:615	the SCAMP2-RFP fusion protein	587:615	Similarly, the SCAMP2-RFP fusion protein, which is localized in TGN, SVC, and plasma membrane (PM), was distributed solely in the PM under the starvation.
32338160	4	11	from	TGN	640:642	arg1	localized					627:635	localized	627:635	localized	627:635	Similarly, the SCAMP2-RFP fusion protein, which is localized in TGN, SVC, and plasma membrane (PM), was distributed solely in the PM under the starvation.
32338160	8	12	theme	plant	1489:1493	arg1	syntaxin					1477:1484	syntaxin	1477:1484	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	0	13	theme	vesicle	92:98	arg1	cluster					100:106	secretory vesicle cluster	82:106	secretory vesicle cluster	82:106	Sucrose starvation induces the degradation of proteins in trans-Golgi network and secretory vesicle cluster in tobacco BY-2 cells.
32338160	5	14	theme	deposition	814:823	arg1	wall					833:836	pectin deposition to cell wall	807:836	pectin deposition to cell wall	807:836	Under the same starvation condition, protein secretion was not arrested but pectin deposition to cell wall was suppressed.
32338160	8	15	theme	yellow	1530:1535	arg1	protein					1549:1555	yellow fluorescent protein	1530:1555	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	8	16	theme	fluorescent	1537:1547	arg1	protein					1549:1555	yellow fluorescent protein	1530:1555	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	0	17	theme	tobacco	111:117	arg1	cells					124:128	tobacco BY-2 cells	111:128	tobacco BY-2 cells	111:128	Sucrose starvation induces the degradation of proteins in trans-Golgi network and secretory vesicle cluster in tobacco BY-2 cells.
32338160	5	18	theme	cell	828:831	arg1	wall					833:836	pectin deposition to cell wall	807:836	pectin deposition to cell wall	807:836	Under the same starvation condition, protein secretion was not arrested but pectin deposition to cell wall was suppressed.
32338160	4	19	theme	SCAMP2-RFP	591:600	arg1	protein					609:615	the SCAMP2-RFP fusion protein	587:615	the SCAMP2-RFP fusion protein	587:615	Similarly, the SCAMP2-RFP fusion protein, which is localized in TGN, SVC, and plasma membrane (PM), was distributed solely in the PM under the starvation.
32338160	4	19	theme	SCAMP2-RFP	591:600	arg1	localized					627:635	localized	627:635	localized	627:635	Similarly, the SCAMP2-RFP fusion protein, which is localized in TGN, SVC, and plasma membrane (PM), was distributed solely in the PM under the starvation.
32338160	3	20	theme	secretory	506:514	arg1	cluster					524:530	the TGN and secretory vesicle cluster	494:530	the TGN and secretory vesicle cluster (SVC)	494:536	The tobacco SNARE protein SYP41, localized in the TGN and secretory vesicle cluster (SVC), also decreased under the starvation.
32338160	3	20	theme	secretory	506:514	arg1	SVC					533:535	SVC	533:535	SVC	533:535	The tobacco SNARE protein SYP41, localized in the TGN and secretory vesicle cluster (SVC), also decreased under the starvation.
32338160	4	21	from	localized	627:635	arg1	SVC					645:647	SVC	645:647	SVC	645:647	Similarly, the SCAMP2-RFP fusion protein, which is localized in TGN, SVC, and plasma membrane (PM), was distributed solely in the PM under the starvation.
32338160	4	21	from	localized	627:635	arg1	TGN					640:642	TGN	640:642	TGN	640:642	Similarly, the SCAMP2-RFP fusion protein, which is localized in TGN, SVC, and plasma membrane (PM), was distributed solely in the PM under the starvation.
32338160	4	21	from	localized	627:635	arg1	membrane					661:668	plasma membrane	654:668	plasma membrane (PM)	654:673	Similarly, the SCAMP2-RFP fusion protein, which is localized in TGN, SVC, and plasma membrane (PM), was distributed solely in the PM under the starvation.
32338160	4	21	from	localized	627:635	arg1	PM					671:672	PM	671:672	PM	671:672	Similarly, the SCAMP2-RFP fusion protein, which is localized in TGN, SVC, and plasma membrane (PM), was distributed solely in the PM under the starvation.
32338160	6	22	theme	sugar	953:957	arg1	availability					959:970	sugar availability	953:970	sugar availability	953:970	These data indicated that the protein composition in TGN and existence of the SVC are regulated by sugar availability.
32338160	7	23	theme	pectin	1036:1041	arg1	secretion					1043:1051	pectin secretion	1036:1051	pectin secretion	1036:1051	Furthermore, our findings as well as the involvement of SVC in pectin secretion suggested that synthesis and transport of pectin are regulated by the level of extracellular sugars.
32338160	5	24	dep	wall	833:836	arg1	to					825:826	to	825:826	to	825:826	Under the same starvation condition, protein secretion was not arrested but pectin deposition to cell wall was suppressed.
32338160	0	25	theme	Sucrose	0:6	arg1	starvation					8:17	Sucrose starvation	0:17	Sucrose starvation	0:17	Sucrose starvation induces the degradation of proteins in trans-Golgi network and secretory vesicle cluster in tobacco BY-2 cells.
32338160	8	26	theme	plasma	1346:1351	arg1	membrane					1353:1360	plasma membrane	1346:1360	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	1	27	theme	endoplasmic	175:185	arg1	reticulum					187:195	the endoplasmic reticulum	171:195	the endoplasmic reticulum (ER)	171:200	Endomembrane transport system begins at the endoplasmic reticulum (ER), continues to the Golgi apparatus and subsequent compartment called trans-Golgi network (TGN).
32338160	1	27	theme	endoplasmic	175:185	arg1	ER					198:199	ER	198:199	ER	198:199	Endomembrane transport system begins at the endoplasmic reticulum (ER), continues to the Golgi apparatus and subsequent compartment called trans-Golgi network (TGN).
32338160	7	28	from	findings	990:997	arg1	secretion					1043:1051	pectin secretion	1036:1051	pectin secretion	1036:1051	Furthermore, our findings as well as the involvement of SVC in pectin secretion suggested that synthesis and transport of pectin are regulated by the level of extracellular sugars.
32338160	1	29	dep	begins	161:166	arg1	continues					203:211	continues	203:211	continues to the Golgi apparatus and subsequent compartment called trans-Golgi network (TGN)	203:294	Endomembrane transport system begins at the endoplasmic reticulum (ER), continues to the Golgi apparatus and subsequent compartment called trans-Golgi network (TGN).
32338160	8	30	theme	protein	1398:1404	arg1	cluster					1461:1467	secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster	1371:1467	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	8	31	theme	ABBREVIATIONS	1154:1166	arg1	protein					1258:1264	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein	1154:1264	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	2	32	theme	sucrose-starvation	418:435	arg1	condition					437:445	a sucrose-starvation condition	416:445	a sucrose-starvation condition	416:445	We found that SUT2, a tobacco sucrose-transporter ortholog and was localized in the TGN, decreased significantly under a sucrose-starvation condition.
32338160	1	33	theme	trans-Golgi	270:280	arg1	TGN					291:293	TGN	291:293	TGN	291:293	Endomembrane transport system begins at the endoplasmic reticulum (ER), continues to the Golgi apparatus and subsequent compartment called trans-Golgi network (TGN).
32338160	1	33	theme	trans-Golgi	270:280	arg1	network					282:288	trans-Golgi network	270:288	trans-Golgi network (TGN)	270:294	Endomembrane transport system begins at the endoplasmic reticulum (ER), continues to the Golgi apparatus and subsequent compartment called trans-Golgi network (TGN).
32338160	8	34	dep	protein	1258:1264	arg1	network					1516:1522	SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network	1363:1522	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	8	34	dep	protein	1258:1264	arg1	PM					1342:1343	PM	1342:1343	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	8	34	dep	protein	1258:1264	arg1	protein					1549:1555	yellow fluorescent protein	1530:1555	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	8	34	dep	protein	1258:1264	arg1	4-hydroxylase					1323:1335	prolyl 4-hydroxylase 1.1	1316:1339	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	8	34	dep	protein	1258:1264	arg1	membrane					1353:1360	plasma membrane	1346:1360	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	8	34	dep	protein	1258:1264	arg1	YFP					1525:1527	YFP	1525:1527	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	6	35	theme	protein	884:890	arg1	composition					892:902	the protein composition	880:902	the protein composition in TGN and existence of the SVC	880:934	These data indicated that the protein composition in TGN and existence of the SVC are regulated by sugar availability.
32338160	8	36	theme	monomeric	1273:1281	arg1	protein					1299:1305	monomeric red fluorescent protein	1273:1305	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	3	37	theme	tobacco	452:458	arg1	protein					466:472	The tobacco SNARE protein SYP41	448:478	The tobacco SNARE protein SYP41	448:478	The tobacco SNARE protein SYP41, localized in the TGN and secretory vesicle cluster (SVC), also decreased under the starvation.
32338160	5	38	theme	same	741:744	arg1	condition					757:765	the same starvation condition	737:765	the same starvation condition	737:765	Under the same starvation condition, protein secretion was not arrested but pectin deposition to cell wall was suppressed.
32338160	0	39	theme	BY-2	119:122	arg1	cells					124:128	tobacco BY-2 cells	111:128	tobacco BY-2 cells	111:128	Sucrose starvation induces the degradation of proteins in trans-Golgi network and secretory vesicle cluster in tobacco BY-2 cells.
32338160	7	40	theme	sugars	1146:1151	arg1	level					1123:1127	the level	1119:1127	the level of extracellular sugars	1119:1151	Furthermore, our findings as well as the involvement of SVC in pectin secretion suggested that synthesis and transport of pectin are regulated by the level of extracellular sugars.
32338160	8	41	theme	trans-Golgi	1504:1514	arg1	network					1516:1522	SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network	1363:1522	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	3	42	theme	SNARE	460:464	arg1	protein					466:472	The tobacco SNARE protein SYP41	448:478	The tobacco SNARE protein SYP41	448:478	The tobacco SNARE protein SYP41, localized in the TGN and secretory vesicle cluster (SVC), also decreased under the starvation.
32338160	8	43	theme	fluorescent	1246:1256	arg1	protein					1258:1264	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein	1154:1264	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	6	44	from	composition	892:902	arg1	existence					915:923	existence	915:923	existence	915:923	These data indicated that the protein composition in TGN and existence of the SVC are regulated by sugar availability.
32338160	6	44	from	composition	892:902	arg1	TGN					907:909	TGN	907:909	TGN	907:909	These data indicated that the protein composition in TGN and existence of the SVC are regulated by sugar availability.
32338160	1	45	dep	apparatus	226:234	arg1	the					216:218	the	216:218	the	216:218	Endomembrane transport system begins at the endoplasmic reticulum (ER), continues to the Golgi apparatus and subsequent compartment called trans-Golgi network (TGN).
32338160	8	46	theme	fluorescent	1287:1297	arg1	protein					1299:1305	monomeric red fluorescent protein	1273:1305	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	8	47	theme	reticulum	1184:1192	arg1	ER					1168:1169	ER	1168:1169	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	8	48	theme	red	1283:1285	arg1	protein					1299:1305	monomeric red fluorescent protein	1273:1305	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	5	49	theme	protein	768:774	arg1	secretion					776:784	protein secretion	768:784	protein secretion	768:784	Under the same starvation condition, protein secretion was not arrested but pectin deposition to cell wall was suppressed.
32338160	8	50	theme	GI-TGN	1195:1200	arg1	ER					1168:1169	ER	1168:1169	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	7	51	from	involvement	1014:1024	arg1	secretion					1043:1051	pectin secretion	1036:1051	pectin secretion	1036:1051	Furthermore, our findings as well as the involvement of SVC in pectin secretion suggested that synthesis and transport of pectin are regulated by the level of extracellular sugars.
32338160	8	52	theme	mRFP	1267:1270	arg1	protein					1258:1264	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein	1154:1264	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	8	53	dep	ABBREVIATIONS	1154:1166	arg1	ER					1168:1169	ER	1168:1169	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	4	54	theme	plasma	654:659	arg1	PM					671:672	PM	671:672	PM	671:672	Similarly, the SCAMP2-RFP fusion protein, which is localized in TGN, SVC, and plasma membrane (PM), was distributed solely in the PM under the starvation.
32338160	4	54	theme	plasma	654:659	arg1	membrane					661:668	plasma membrane	654:668	plasma membrane (PM)	654:673	Similarly, the SCAMP2-RFP fusion protein, which is localized in TGN, SVC, and plasma membrane (PM), was distributed solely in the PM under the starvation.
32338160	0	55	theme	proteins	46:53	arg1	degradation					31:41	the degradation	27:41	the degradation of proteins	27:53	Sucrose starvation induces the degradation of proteins in trans-Golgi network and secretory vesicle cluster in tobacco BY-2 cells.
32338160	8	56	theme	secretory	1443:1451	arg1	cluster					1461:1467	secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster	1371:1467	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	7	57	theme	pectin	1095:1100	arg1	synthesis					1068:1076	synthesis	1068:1076	synthesis	1068:1076	Furthermore, our findings as well as the involvement of SVC in pectin secretion suggested that synthesis and transport of pectin are regulated by the level of extracellular sugars.
32338160	7	57	theme	pectin	1095:1100	arg1	transport					1082:1090	transport	1082:1090	transport	1082:1090	Furthermore, our findings as well as the involvement of SVC in pectin secretion suggested that synthesis and transport of pectin are regulated by the level of extracellular sugars.
32338160	8	58	theme	protein	1299:1305	arg1	protein					1258:1264	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein	1154:1264	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	8	59	dep	SCAMP2	1363:1368	arg1	TGN					1499:1501	TGN	1499:1501	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	8	59	dep	SCAMP2	1363:1368	arg1	syntaxin					1477:1484	syntaxin	1477:1484	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	8	59	dep	SCAMP2	1363:1368	arg1	SYP41					1470:1474	SYP41	1470:1474	SYP41	1470:1474	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	8	59	dep	SCAMP2	1363:1368	arg1	cluster					1461:1467	secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster	1371:1467	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	8	60	theme	prolyl	1316:1321	arg1	4-hydroxylase					1323:1335	prolyl 4-hydroxylase 1.1	1316:1339	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	8	61	theme	sucrose	1415:1421	arg1	transporter					1423:1433	sucrose transporter 2	1415:1435	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	2	62	dep	localized	364:372	arg1	decreased					386:394	decreased	386:394	decreased significantly under a sucrose-starvation condition	386:445	We found that SUT2, a tobacco sucrose-transporter ortholog and was localized in the TGN, decreased significantly under a sucrose-starvation condition.
32338160	8	63	theme	Golgi-released	1203:1216	arg1	TGN					1230:1232	Golgi-released independent TGN	1203:1232	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	4	64	from	membrane	661:668	arg1	protein					609:615	the SCAMP2-RFP fusion protein	587:615	the SCAMP2-RFP fusion protein	587:615	Similarly, the SCAMP2-RFP fusion protein, which is localized in TGN, SVC, and plasma membrane (PM), was distributed solely in the PM under the starvation.
32338160	4	64	from	membrane	661:668	arg1	localized					627:635	localized	627:635	localized	627:635	Similarly, the SCAMP2-RFP fusion protein, which is localized in TGN, SVC, and plasma membrane (PM), was distributed solely in the PM under the starvation.
32338160	8	65	theme	endoplasmic	1172:1182	arg1	reticulum					1184:1192	endoplasmic reticulum	1172:1192	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	3	66	theme	vesicle	516:522	arg1	cluster					524:530	the TGN and secretory vesicle cluster	494:530	the TGN and secretory vesicle cluster (SVC)	494:536	The tobacco SNARE protein SYP41, localized in the TGN and secretory vesicle cluster (SVC), also decreased under the starvation.
32338160	3	66	theme	vesicle	516:522	arg1	SVC					533:535	SVC	533:535	SVC	533:535	The tobacco SNARE protein SYP41, localized in the TGN and secretory vesicle cluster (SVC), also decreased under the starvation.
32338160	8	67	theme	SCAMP2	1363:1368	arg1	network					1516:1522	SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network	1363:1522	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	8	68	theme	TGN	1230:1232	arg1	ER					1168:1169	ER	1168:1169	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	3	69	located	localized	481:489	arg1	cluster					524:530	the TGN and secretory vesicle cluster	494:530	the TGN and secretory vesicle cluster (SVC)	494:536	The tobacco SNARE protein SYP41, localized in the TGN and secretory vesicle cluster (SVC), also decreased under the starvation.
32338160	3	69	located	localized	481:489	arg2	protein					466:472	The tobacco SNARE protein SYP41	448:478	The tobacco SNARE protein SYP41	448:478	The tobacco SNARE protein SYP41, localized in the TGN and secretory vesicle cluster (SVC), also decreased under the starvation.
32338160	3	69	located	localized	481:489	arg1	SVC					533:535	SVC	533:535	SVC	533:535	The tobacco SNARE protein SYP41, localized in the TGN and secretory vesicle cluster (SVC), also decreased under the starvation.
32338160	1	70	theme	Endomembrane	131:142	arg1	system					154:159	Endomembrane transport system	131:159	Endomembrane transport system	131:159	Endomembrane transport system begins at the endoplasmic reticulum (ER), continues to the Golgi apparatus and subsequent compartment called trans-Golgi network (TGN).
32338160	8	71	theme	GFP	1235:1237	arg1	ER					1168:1169	ER	1168:1169	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	6	72	theme	SVC	932:934	arg1	existence					915:923	existence	915:923	existence	915:923	These data indicated that the protein composition in TGN and existence of the SVC are regulated by sugar availability.
32338160	6	72	theme	SVC	932:934	arg1	TGN					907:909	TGN	907:909	TGN	907:909	These data indicated that the protein composition in TGN and existence of the SVC are regulated by sugar availability.
32338160	2	73	located	localized	364:372	arg2	ortholog					347:354	a tobacco sucrose-transporter ortholog	317:354	a tobacco sucrose-transporter ortholog	317:354	We found that SUT2, a tobacco sucrose-transporter ortholog and was localized in the TGN, decreased significantly under a sucrose-starvation condition.
32338160	2	73	located	localized	364:372	arg2	SUT2					311:314	SUT2	311:314	SUT2	311:314	We found that SUT2, a tobacco sucrose-transporter ortholog and was localized in the TGN, decreased significantly under a sucrose-starvation condition.
32338160	2	73	located	localized	364:372	arg1	TGN					381:383	the TGN	377:383	the TGN	377:383	We found that SUT2, a tobacco sucrose-transporter ortholog and was localized in the TGN, decreased significantly under a sucrose-starvation condition.
32338160	4	74	theme	fusion	602:607	arg1	protein					609:615	the SCAMP2-RFP fusion protein	587:615	the SCAMP2-RFP fusion protein	587:615	Similarly, the SCAMP2-RFP fusion protein, which is localized in TGN, SVC, and plasma membrane (PM), was distributed solely in the PM under the starvation.
32338160	4	74	theme	fusion	602:607	arg1	localized					627:635	localized	627:635	localized	627:635	Similarly, the SCAMP2-RFP fusion protein, which is localized in TGN, SVC, and plasma membrane (PM), was distributed solely in the PM under the starvation.
32338160	1	75	theme	transport	144:152	arg1	system					154:159	Endomembrane transport system	131:159	Endomembrane transport system	131:159	Endomembrane transport system begins at the endoplasmic reticulum (ER), continues to the Golgi apparatus and subsequent compartment called trans-Golgi network (TGN).
32338160	1	76	theme	Golgi	220:224	arg1	apparatus					226:234	Golgi apparatus	220:234	Golgi apparatus	220:234	Endomembrane transport system begins at the endoplasmic reticulum (ER), continues to the Golgi apparatus and subsequent compartment called trans-Golgi network (TGN).
32338160	0	77	theme	trans-Golgi	58:68	arg1	network					70:76	trans-Golgi network	58:76	trans-Golgi network	58:76	Sucrose starvation induces the degradation of proteins in trans-Golgi network and secretory vesicle cluster in tobacco BY-2 cells.
32338160	8	78	theme	secretory	1371:1379	arg1	protein					1398:1404	secretory carrier membrane protein 2	1371:1406	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	8	79	theme	vesicle	1453:1459	arg1	cluster					1461:1467	secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster	1371:1467	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	5	80	theme	pectin	807:812	arg1	wall					833:836	pectin deposition to cell wall	807:836	pectin deposition to cell wall	807:836	Under the same starvation condition, protein secretion was not arrested but pectin deposition to cell wall was suppressed.
32338160	7	81	theme	extracellular	1132:1144	arg1	sugars					1146:1151	extracellular sugars	1132:1151	extracellular sugars	1132:1151	Furthermore, our findings as well as the involvement of SVC in pectin secretion suggested that synthesis and transport of pectin are regulated by the level of extracellular sugars.
32338160	8	82	dep	protein	1398:1404	arg1	transporter					1423:1433	sucrose transporter 2	1415:1435	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	8	82	dep	protein	1398:1404	arg1	SVC					1438:1440	SVC	1438:1440	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32338160	8	82	dep	protein	1398:1404	arg1	SUT2					1409:1412	SUT2	1409:1412	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.	1154:1556	ABBREVIATIONS ER: endoplasmic reticulum; GI-TGN: Golgi-released independent TGN; GFP: green fluorescent protein; mRFP: monomeric red fluorescent protein; P4H1.1: prolyl 4-hydroxylase 1.1; PM: plasma membrane; SCAMP2: secretory carrier membrane protein 2; SUT2: sucrose transporter 2; SVC: secretory vesicle cluster; SYP41: syntaxin of plant 41; TGN: trans-Golgi network; YFP: yellow fluorescent protein.
32656096	4	0	theme	selected	888:895	arg1	markers					905:911	six selected species markers	884:911	six selected species markers in a subset of validation cohort of 45 HPV16 positive women and 53 controls	884:987	We further employed qPCR assays to evaluate two selected gene markers of HPV16 infection in an independent validation cohort consisting of 88 HPV16 positive women and 81 controls, and six selected species markers in a subset of validation cohort of 45 HPV16 positive women and 53 controls.
32656096	3	1	theme	HPV	499:501	arg1	controls					512:519	25 age-matched HPV negative controls	484:519	25 age-matched HPV negative controls	484:519	Here we identified the compositional and functional alterations on vaginal samples from 27 HPV16 positive women and 25 age-matched HPV negative controls using shotgun metagenomic sequencing, to provide a comprehensive investigation describing the microbial abundances and enriched metabolic functions in cervicovaginal metagenomes.
32656096	3	2	from	functions	659:667	arg1	metagenomes					687:697	cervicovaginal metagenomes	672:697	cervicovaginal metagenomes	672:697	Here we identified the compositional and functional alterations on vaginal samples from 27 HPV16 positive women and 25 age-matched HPV negative controls using shotgun metagenomic sequencing, to provide a comprehensive investigation describing the microbial abundances and enriched metabolic functions in cervicovaginal metagenomes.
32656096	9	3	theme	genital	2161:2167	arg1	tract					2169:2173	the female genital tract	2150:2173	the female genital tract	2150:2173	These results highlight a fundamental fact that there are altered composition and function of the vaginal microbiome in HPV16-positive women, suggesting that vaginal dysbiosis may be associated with HPV infection in the female genital tract.
32656096	2	4	theme	cancer	264:269	arg1	initiation					271:280	cervical cancer initiation	255:280	cervical cancer initiation	255:280	Persistent HPV infection is a causal event in cervical cancer initiation, but, little is known about the microbiome composition and function in HPV infection.
32656096	6	5	theme	area	1520:1523	arg1	0.861					1474:1478	0.861	1474:1478	0.861	1474:1478	Abundance of 12 genes, 17 genera, and 7 species biomarkers showed an excellent predictive power for the HPV16-positive individuals, with 0.861, 0.819, and 0.918, respectively, of the area under the receiver-operating characteristic curve (AUC).
32656096	6	5	theme	area	1520:1523	arg1	area					1520:1523	the area	1516:1523	the area under the receiver-operating characteristic curve (AUC)	1516:1579	Abundance of 12 genes, 17 genera, and 7 species biomarkers showed an excellent predictive power for the HPV16-positive individuals, with 0.861, 0.819, and 0.918, respectively, of the area under the receiver-operating characteristic curve (AUC).
32656096	4	6	theme	cohort	939:944	arg1	subset					918:923	a subset	916:923	a subset of validation cohort of 45 HPV16 positive women and 53 controls	916:987	We further employed qPCR assays to evaluate two selected gene markers of HPV16 infection in an independent validation cohort consisting of 88 HPV16 positive women and 81 controls, and six selected species markers in a subset of validation cohort of 45 HPV16 positive women and 53 controls.
32656096	6	7	theme	genes	1353:1357	arg1	Abundance					1337:1345	Abundance	1337:1345	Abundance of 12 genes, 17 genera, and 7 species biomarkers	1337:1394	Abundance of 12 genes, 17 genera, and 7 species biomarkers showed an excellent predictive power for the HPV16-positive individuals, with 0.861, 0.819, and 0.918, respectively, of the area under the receiver-operating characteristic curve (AUC).
32656096	1	8	theme	hotspots	199:206	arg1	hotspots					199:206	the research hotspots	186:206	the research hotspots	186:206	The association of microbiome imbalance with cancer development is being one of the research hotspots.
32656096	1	8	theme	hotspots	199:206	arg1	association					110:120	The association	106:120	The association of microbiome imbalance with cancer development	106:168	The association of microbiome imbalance with cancer development is being one of the research hotspots.
32656096	1	8	theme	hotspots	199:206	arg1	one					179:181	one	179:181	one	179:181	The association of microbiome imbalance with cancer development is being one of the research hotspots.
32656096	7	9	theme	microbial	1611:1619	arg1	function					1621:1628	the microbial function	1607:1628	the microbial function	1607:1628	We further characterized the microbial function, and revealed that HPV16-positive women were enriched in metabolism and membrane transport, and depleted by glycan biosynthesis and metabolism, and replication and repair.
32656096	2	10	theme	HPV	220:222	arg1	event					246:250	a causal event	237:250	a causal event in cervical cancer initiation	237:280	Persistent HPV infection is a causal event in cervical cancer initiation, but, little is known about the microbiome composition and function in HPV infection.
32656096	2	10	theme	HPV	220:222	arg1	infection					224:232	Persistent HPV infection	209:232	Persistent HPV infection	209:232	Persistent HPV infection is a causal event in cervical cancer initiation, but, little is known about the microbiome composition and function in HPV infection.
32656096	7	11	theme	glycan	1738:1743	arg1	biosynthesis					1745:1756	glycan biosynthesis	1738:1756	glycan biosynthesis	1738:1756	We further characterized the microbial function, and revealed that HPV16-positive women were enriched in metabolism and membrane transport, and depleted by glycan biosynthesis and metabolism, and replication and repair.
32656096	8	12	theme	gene	1851:1854	arg1	marker					1856:1861	one gene marker	1847:1861	one gene marker	1847:1861	Quantitative PCR measurements validated that one gene marker and three species were significantly enriched in HPV16-positive women.
32656096	9	13	theme	altered	1992:1998	arg1	composition					2000:2010	altered composition	1992:2010	altered composition	1992:2010	These results highlight a fundamental fact that there are altered composition and function of the vaginal microbiome in HPV16-positive women, suggesting that vaginal dysbiosis may be associated with HPV infection in the female genital tract.
32656096	4	14	theme	gene	757:760	arg1	markers					762:768	two selected gene markers	744:768	two selected gene markers of HPV16 infection in an independent validation cohort consisting of 88 HPV16 positive women and 81 controls	744:877	We further employed qPCR assays to evaluate two selected gene markers of HPV16 infection in an independent validation cohort consisting of 88 HPV16 positive women and 81 controls, and six selected species markers in a subset of validation cohort of 45 HPV16 positive women and 53 controls.
32656096	4	15	theme	infection	779:787	arg1	markers					762:768	two selected gene markers	744:768	two selected gene markers of HPV16 infection in an independent validation cohort consisting of 88 HPV16 positive women and 81 controls	744:877	We further employed qPCR assays to evaluate two selected gene markers of HPV16 infection in an independent validation cohort consisting of 88 HPV16 positive women and 81 controls, and six selected species markers in a subset of validation cohort of 45 HPV16 positive women and 53 controls.
32656096	4	15	theme	infection	779:787	arg1	markers					905:911	six selected species markers	884:911	six selected species markers in a subset of validation cohort of 45 HPV16 positive women and 53 controls	884:987	We further employed qPCR assays to evaluate two selected gene markers of HPV16 infection in an independent validation cohort consisting of 88 HPV16 positive women and 81 controls, and six selected species markers in a subset of validation cohort of 45 HPV16 positive women and 53 controls.
32656096	3	16	theme	enriched	640:647	arg1	functions					659:667	enriched metabolic functions	640:667	enriched metabolic functions in cervicovaginal metagenomes	640:697	Here we identified the compositional and functional alterations on vaginal samples from 27 HPV16 positive women and 25 age-matched HPV negative controls using shotgun metagenomic sequencing, to provide a comprehensive investigation describing the microbial abundances and enriched metabolic functions in cervicovaginal metagenomes.
32656096	1	17	theme	imbalance	136:144	arg1	hotspots					199:206	the research hotspots	186:206	the research hotspots	186:206	The association of microbiome imbalance with cancer development is being one of the research hotspots.
32656096	1	17	theme	imbalance	136:144	arg1	association					110:120	The association	106:120	The association of microbiome imbalance with cancer development	106:168	The association of microbiome imbalance with cancer development is being one of the research hotspots.
32656096	1	17	theme	imbalance	136:144	arg1	one					179:181	one	179:181	one	179:181	The association of microbiome imbalance with cancer development is being one of the research hotspots.
32656096	4	18	theme	controls	980:987	arg1	cohort					939:944	validation cohort	928:944	validation cohort of 45 HPV16 positive women and 53 controls	928:987	We further employed qPCR assays to evaluate two selected gene markers of HPV16 infection in an independent validation cohort consisting of 88 HPV16 positive women and 81 controls, and six selected species markers in a subset of validation cohort of 45 HPV16 positive women and 53 controls.
32656096	4	19	theme	qPCR	720:723	arg1	assays					725:730	qPCR assays	720:730	qPCR assays	720:730	We further employed qPCR assays to evaluate two selected gene markers of HPV16 infection in an independent validation cohort consisting of 88 HPV16 positive women and 81 controls, and six selected species markers in a subset of validation cohort of 45 HPV16 positive women and 53 controls.
32656096	6	20	theme	biomarkers	1385:1394	arg1	Abundance					1337:1345	Abundance	1337:1345	Abundance of 12 genes, 17 genera, and 7 species biomarkers	1337:1394	Abundance of 12 genes, 17 genera, and 7 species biomarkers showed an excellent predictive power for the HPV16-positive individuals, with 0.861, 0.819, and 0.918, respectively, of the area under the receiver-operating characteristic curve (AUC).
32656096	4	21	from	markers	905:911	arg1	subset					918:923	a subset	916:923	a subset of validation cohort of 45 HPV16 positive women and 53 controls	916:987	We further employed qPCR assays to evaluate two selected gene markers of HPV16 infection in an independent validation cohort consisting of 88 HPV16 positive women and 81 controls, and six selected species markers in a subset of validation cohort of 45 HPV16 positive women and 53 controls.
32656096	4	21	from	markers	905:911	arg1	cohort					818:823	an independent validation cohort	792:823	an independent validation cohort consisting of 88 HPV16 positive women and 81 controls	792:877	We further employed qPCR assays to evaluate two selected gene markers of HPV16 infection in an independent validation cohort consisting of 88 HPV16 positive women and 81 controls, and six selected species markers in a subset of validation cohort of 45 HPV16 positive women and 53 controls.
32656096	2	22	theme	causal	239:244	arg1	event					246:250	a causal event	237:250	a causal event in cervical cancer initiation	237:280	Persistent HPV infection is a causal event in cervical cancer initiation, but, little is known about the microbiome composition and function in HPV infection.
32656096	2	22	theme	causal	239:244	arg1	infection					224:232	Persistent HPV infection	209:232	Persistent HPV infection	209:232	Persistent HPV infection is a causal event in cervical cancer initiation, but, little is known about the microbiome composition and function in HPV infection.
32656096	6	23	theme	characteristic	1554:1567	arg1	curve					1569:1573	the receiver-operating characteristic curve	1531:1573	the receiver-operating characteristic curve (AUC)	1531:1579	Abundance of 12 genes, 17 genera, and 7 species biomarkers showed an excellent predictive power for the HPV16-positive individuals, with 0.861, 0.819, and 0.918, respectively, of the area under the receiver-operating characteristic curve (AUC).
32656096	6	23	theme	characteristic	1554:1567	arg1	AUC					1576:1578	AUC	1576:1578	AUC	1576:1578	Abundance of 12 genes, 17 genera, and 7 species biomarkers showed an excellent predictive power for the HPV16-positive individuals, with 0.861, 0.819, and 0.918, respectively, of the area under the receiver-operating characteristic curve (AUC).
32656096	1	24	theme	cancer	151:156	arg1	development					158:168	cancer development	151:168	cancer development	151:168	The association of microbiome imbalance with cancer development is being one of the research hotspots.
32656096	5	25	theme	HPV16-positive	1141:1154	arg1	group					1156:1160	the HPV16-positive group	1137:1160	the HPV16-positive group	1137:1160	We found that the relative abundance of dominant Firmicutes was lower, Actinobacteria, Fusobacteria and viruses phyla were significantly higher in the HPV16-positive group; 77 genera including Gardnerella, Peptostreptococcus, and Prevotella were higher, and 20 genera including Lactobacillus and Aerococcus were lower in the HPV16-positive women.
32656096	8	26	theme	PCR	1815:1817	arg1	measurements					1819:1830	Quantitative PCR measurements	1802:1830	Quantitative PCR measurements	1802:1830	Quantitative PCR measurements validated that one gene marker and three species were significantly enriched in HPV16-positive women.
32656096	7	27	theme	membrane	1702:1709	arg1	transport					1711:1719	membrane transport	1702:1719	membrane transport	1702:1719	We further characterized the microbial function, and revealed that HPV16-positive women were enriched in metabolism and membrane transport, and depleted by glycan biosynthesis and metabolism, and replication and repair.
32656096	4	28	theme	positive	958:965	arg1	women					967:971	45 HPV16 positive women	949:971	45 HPV16 positive women	949:971	We further employed qPCR assays to evaluate two selected gene markers of HPV16 infection in an independent validation cohort consisting of 88 HPV16 positive women and 81 controls, and six selected species markers in a subset of validation cohort of 45 HPV16 positive women and 53 controls.
32656096	9	29	theme	vaginal	2032:2038	arg1	microbiome					2040:2049	the vaginal microbiome	2028:2049	the vaginal microbiome	2028:2049	These results highlight a fundamental fact that there are altered composition and function of the vaginal microbiome in HPV16-positive women, suggesting that vaginal dysbiosis may be associated with HPV infection in the female genital tract.
32656096	0	30	theme	Metagenomic	82:92	arg1	Sequencing					94:103	Shotgun Metagenomic Sequencing	74:103	Shotgun Metagenomic Sequencing	74:103	The Alterations of Vaginal Microbiome in HPV16 Infection as Identified by Shotgun Metagenomic Sequencing.
32656096	4	31	theme	validation	807:816	arg1	cohort					818:823	an independent validation cohort	792:823	an independent validation cohort consisting of 88 HPV16 positive women and 81 controls	792:877	We further employed qPCR assays to evaluate two selected gene markers of HPV16 infection in an independent validation cohort consisting of 88 HPV16 positive women and 81 controls, and six selected species markers in a subset of validation cohort of 45 HPV16 positive women and 53 controls.
32656096	3	32	from	abundances	625:634	arg1	metagenomes					687:697	cervicovaginal metagenomes	672:697	cervicovaginal metagenomes	672:697	Here we identified the compositional and functional alterations on vaginal samples from 27 HPV16 positive women and 25 age-matched HPV negative controls using shotgun metagenomic sequencing, to provide a comprehensive investigation describing the microbial abundances and enriched metabolic functions in cervicovaginal metagenomes.
32656096	4	33	from	infection	779:787	arg1	cohort					818:823	an independent validation cohort	792:823	an independent validation cohort consisting of 88 HPV16 positive women and 81 controls	792:877	We further employed qPCR assays to evaluate two selected gene markers of HPV16 infection in an independent validation cohort consisting of 88 HPV16 positive women and 81 controls, and six selected species markers in a subset of validation cohort of 45 HPV16 positive women and 53 controls.
32656096	3	34	theme	functional	409:418	arg1	alterations					420:430	the compositional and functional alterations	387:430	the compositional and functional alterations on vaginal samples from 27 HPV16 positive women and 25 age-matched HPV negative controls	387:519	Here we identified the compositional and functional alterations on vaginal samples from 27 HPV16 positive women and 25 age-matched HPV negative controls using shotgun metagenomic sequencing, to provide a comprehensive investigation describing the microbial abundances and enriched metabolic functions in cervicovaginal metagenomes.
32656096	6	35	theme	HPV16-positive	1441:1454	arg1	individuals					1456:1466	the HPV16-positive individuals	1437:1466	the HPV16-positive individuals	1437:1466	Abundance of 12 genes, 17 genera, and 7 species biomarkers showed an excellent predictive power for the HPV16-positive individuals, with 0.861, 0.819, and 0.918, respectively, of the area under the receiver-operating characteristic curve (AUC).
32656096	2	36	from	event	246:250	arg1	initiation					271:280	cervical cancer initiation	255:280	cervical cancer initiation	255:280	Persistent HPV infection is a causal event in cervical cancer initiation, but, little is known about the microbiome composition and function in HPV infection.
32656096	9	37	theme	vaginal	2092:2098	arg1	dysbiosis					2100:2108	vaginal dysbiosis	2092:2108	vaginal dysbiosis	2092:2108	These results highlight a fundamental fact that there are altered composition and function of the vaginal microbiome in HPV16-positive women, suggesting that vaginal dysbiosis may be associated with HPV infection in the female genital tract.
32656096	2	38	dep	composition	325:335	arg1	the					310:312	the	310:312	the	310:312	Persistent HPV infection is a causal event in cervical cancer initiation, but, little is known about the microbiome composition and function in HPV infection.
32656096	3	39	theme	compositional	391:403	arg1	alterations					420:430	the compositional and functional alterations	387:430	the compositional and functional alterations on vaginal samples from 27 HPV16 positive women and 25 age-matched HPV negative controls	387:519	Here we identified the compositional and functional alterations on vaginal samples from 27 HPV16 positive women and 25 age-matched HPV negative controls using shotgun metagenomic sequencing, to provide a comprehensive investigation describing the microbial abundances and enriched metabolic functions in cervicovaginal metagenomes.
32656096	3	40	theme	cervicovaginal	672:685	arg1	metagenomes					687:697	cervicovaginal metagenomes	672:697	cervicovaginal metagenomes	672:697	Here we identified the compositional and functional alterations on vaginal samples from 27 HPV16 positive women and 25 age-matched HPV negative controls using shotgun metagenomic sequencing, to provide a comprehensive investigation describing the microbial abundances and enriched metabolic functions in cervicovaginal metagenomes.
32656096	4	41	theme	positive	848:855	arg1	women					857:861	88 HPV16 positive women	839:861	88 HPV16 positive women	839:861	We further employed qPCR assays to evaluate two selected gene markers of HPV16 infection in an independent validation cohort consisting of 88 HPV16 positive women and 81 controls, and six selected species markers in a subset of validation cohort of 45 HPV16 positive women and 53 controls.
32656096	0	42	theme	Microbiome	27:36	arg1	Alterations					4:14	The Alterations	0:14	The Alterations of Vaginal Microbiome in HPV16 Infection	0:55	The Alterations of Vaginal Microbiome in HPV16 Infection as Identified by Shotgun Metagenomic Sequencing.
32656096	6	43	theme	predictive	1416:1425	arg1	power					1427:1431	an excellent predictive power	1403:1431	an excellent predictive power for the HPV16-positive individuals	1403:1466	Abundance of 12 genes, 17 genera, and 7 species biomarkers showed an excellent predictive power for the HPV16-positive individuals, with 0.861, 0.819, and 0.918, respectively, of the area under the receiver-operating characteristic curve (AUC).
32656096	0	44	theme	HPV16	41:45	arg1	Infection					47:55	HPV16 Infection	41:55	HPV16 Infection	41:55	The Alterations of Vaginal Microbiome in HPV16 Infection as Identified by Shotgun Metagenomic Sequencing.
32656096	5	45	theme	Firmicutes	1039:1048	arg1	higher					1127:1132	higher	1127:1132	higher	1127:1132	We found that the relative abundance of dominant Firmicutes was lower, Actinobacteria, Fusobacteria and viruses phyla were significantly higher in the HPV16-positive group; 77 genera including Gardnerella, Peptostreptococcus, and Prevotella were higher, and 20 genera including Lactobacillus and Aerococcus were lower in the HPV16-positive women.
32656096	5	45	theme	Firmicutes	1039:1048	arg1	abundance					1017:1025	the relative abundance	1004:1025	the relative abundance of dominant Firmicutes was lower, Actinobacteria, Fusobacteria and viruses phyla	1004:1106	We found that the relative abundance of dominant Firmicutes was lower, Actinobacteria, Fusobacteria and viruses phyla were significantly higher in the HPV16-positive group; 77 genera including Gardnerella, Peptostreptococcus, and Prevotella were higher, and 20 genera including Lactobacillus and Aerococcus were lower in the HPV16-positive women.
32656096	3	46	theme	positive	465:472	arg1	women					474:478	27 HPV16 positive women	456:478	27 HPV16 positive women	456:478	Here we identified the compositional and functional alterations on vaginal samples from 27 HPV16 positive women and 25 age-matched HPV negative controls using shotgun metagenomic sequencing, to provide a comprehensive investigation describing the microbial abundances and enriched metabolic functions in cervicovaginal metagenomes.
32656096	3	47	theme	shotgun	527:533	arg1	sequencing					547:556	shotgun metagenomic sequencing	527:556	shotgun metagenomic sequencing	527:556	Here we identified the compositional and functional alterations on vaginal samples from 27 HPV16 positive women and 25 age-matched HPV negative controls using shotgun metagenomic sequencing, to provide a comprehensive investigation describing the microbial abundances and enriched metabolic functions in cervicovaginal metagenomes.
32656096	9	48	theme	HPV	2133:2135	arg1	infection					2137:2145	HPV infection	2133:2145	HPV infection in the female genital tract	2133:2173	These results highlight a fundamental fact that there are altered composition and function of the vaginal microbiome in HPV16-positive women, suggesting that vaginal dysbiosis may be associated with HPV infection in the female genital tract.
32656096	3	49	from	alterations	420:430	arg1	samples					443:449	vaginal samples	435:449	vaginal samples from 27 HPV16 positive women and 25 age-matched HPV negative controls	435:519	Here we identified the compositional and functional alterations on vaginal samples from 27 HPV16 positive women and 25 age-matched HPV negative controls using shotgun metagenomic sequencing, to provide a comprehensive investigation describing the microbial abundances and enriched metabolic functions in cervicovaginal metagenomes.
32656096	3	50	theme	negative	503:510	arg1	controls					512:519	25 age-matched HPV negative controls	484:519	25 age-matched HPV negative controls	484:519	Here we identified the compositional and functional alterations on vaginal samples from 27 HPV16 positive women and 25 age-matched HPV negative controls using shotgun metagenomic sequencing, to provide a comprehensive investigation describing the microbial abundances and enriched metabolic functions in cervicovaginal metagenomes.
32656096	9	51	from	infection	2137:2145	arg1	tract					2169:2173	the female genital tract	2150:2173	the female genital tract	2150:2173	These results highlight a fundamental fact that there are altered composition and function of the vaginal microbiome in HPV16-positive women, suggesting that vaginal dysbiosis may be associated with HPV infection in the female genital tract.
32656096	5	52	theme	relative	1008:1015	arg1	higher					1127:1132	higher	1127:1132	higher	1127:1132	We found that the relative abundance of dominant Firmicutes was lower, Actinobacteria, Fusobacteria and viruses phyla were significantly higher in the HPV16-positive group; 77 genera including Gardnerella, Peptostreptococcus, and Prevotella were higher, and 20 genera including Lactobacillus and Aerococcus were lower in the HPV16-positive women.
32656096	5	52	theme	relative	1008:1015	arg1	abundance					1017:1025	the relative abundance	1004:1025	the relative abundance of dominant Firmicutes was lower, Actinobacteria, Fusobacteria and viruses phyla	1004:1106	We found that the relative abundance of dominant Firmicutes was lower, Actinobacteria, Fusobacteria and viruses phyla were significantly higher in the HPV16-positive group; 77 genera including Gardnerella, Peptostreptococcus, and Prevotella were higher, and 20 genera including Lactobacillus and Aerococcus were lower in the HPV16-positive women.
32656096	6	53	theme	species	1377:1383	arg1	biomarkers					1385:1394	7 species biomarkers	1375:1394	7 species biomarkers	1375:1394	Abundance of 12 genes, 17 genera, and 7 species biomarkers showed an excellent predictive power for the HPV16-positive individuals, with 0.861, 0.819, and 0.918, respectively, of the area under the receiver-operating characteristic curve (AUC).
32656096	3	54	theme	age-matched	487:497	arg1	controls					512:519	25 age-matched HPV negative controls	484:519	25 age-matched HPV negative controls	484:519	Here we identified the compositional and functional alterations on vaginal samples from 27 HPV16 positive women and 25 age-matched HPV negative controls using shotgun metagenomic sequencing, to provide a comprehensive investigation describing the microbial abundances and enriched metabolic functions in cervicovaginal metagenomes.
32656096	9	55	theme	female	2154:2159	arg1	tract					2169:2173	the female genital tract	2150:2173	the female genital tract	2150:2173	These results highlight a fundamental fact that there are altered composition and function of the vaginal microbiome in HPV16-positive women, suggesting that vaginal dysbiosis may be associated with HPV infection in the female genital tract.
32656096	3	56	theme	metagenomic	535:545	arg1	sequencing					547:556	shotgun metagenomic sequencing	527:556	shotgun metagenomic sequencing	527:556	Here we identified the compositional and functional alterations on vaginal samples from 27 HPV16 positive women and 25 age-matched HPV negative controls using shotgun metagenomic sequencing, to provide a comprehensive investigation describing the microbial abundances and enriched metabolic functions in cervicovaginal metagenomes.
32656096	6	57	theme	receiver-operating	1535:1552	arg1	curve					1569:1573	the receiver-operating characteristic curve	1531:1573	the receiver-operating characteristic curve (AUC)	1531:1579	Abundance of 12 genes, 17 genera, and 7 species biomarkers showed an excellent predictive power for the HPV16-positive individuals, with 0.861, 0.819, and 0.918, respectively, of the area under the receiver-operating characteristic curve (AUC).
32656096	6	57	theme	receiver-operating	1535:1552	arg1	AUC					1576:1578	AUC	1576:1578	AUC	1576:1578	Abundance of 12 genes, 17 genera, and 7 species biomarkers showed an excellent predictive power for the HPV16-positive individuals, with 0.861, 0.819, and 0.918, respectively, of the area under the receiver-operating characteristic curve (AUC).
32656096	4	58	theme	species	897:903	arg1	markers					905:911	six selected species markers	884:911	six selected species markers in a subset of validation cohort of 45 HPV16 positive women and 53 controls	884:987	We further employed qPCR assays to evaluate two selected gene markers of HPV16 infection in an independent validation cohort consisting of 88 HPV16 positive women and 81 controls, and six selected species markers in a subset of validation cohort of 45 HPV16 positive women and 53 controls.
32656096	2	59	from	composition	325:335	arg1	infection					357:365	HPV infection	353:365	HPV infection	353:365	Persistent HPV infection is a causal event in cervical cancer initiation, but, little is known about the microbiome composition and function in HPV infection.
32656096	3	60	from	controls	512:519	arg1	samples					443:449	vaginal samples	435:449	vaginal samples from 27 HPV16 positive women and 25 age-matched HPV negative controls	435:519	Here we identified the compositional and functional alterations on vaginal samples from 27 HPV16 positive women and 25 age-matched HPV negative controls using shotgun metagenomic sequencing, to provide a comprehensive investigation describing the microbial abundances and enriched metabolic functions in cervicovaginal metagenomes.
32656096	2	61	from	function	341:348	arg1	infection					357:365	HPV infection	353:365	HPV infection	353:365	Persistent HPV infection is a causal event in cervical cancer initiation, but, little is known about the microbiome composition and function in HPV infection.
32656096	5	62	dep	Firmicutes	1039:1048	arg1	phyla					1102:1106	phyla	1102:1106	phyla	1102:1106	We found that the relative abundance of dominant Firmicutes was lower, Actinobacteria, Fusobacteria and viruses phyla were significantly higher in the HPV16-positive group; 77 genera including Gardnerella, Peptostreptococcus, and Prevotella were higher, and 20 genera including Lactobacillus and Aerococcus were lower in the HPV16-positive women.
32656096	8	63	theme	HPV16-positive	1912:1925	arg1	women					1927:1931	HPV16-positive women	1912:1931	HPV16-positive women	1912:1931	Quantitative PCR measurements validated that one gene marker and three species were significantly enriched in HPV16-positive women.
32656096	3	64	from	women	474:478	arg1	samples					443:449	vaginal samples	435:449	vaginal samples from 27 HPV16 positive women and 25 age-matched HPV negative controls	435:519	Here we identified the compositional and functional alterations on vaginal samples from 27 HPV16 positive women and 25 age-matched HPV negative controls using shotgun metagenomic sequencing, to provide a comprehensive investigation describing the microbial abundances and enriched metabolic functions in cervicovaginal metagenomes.
32656096	1	65	theme	research	190:197	arg1	hotspots					199:206	the research hotspots	186:206	the research hotspots	186:206	The association of microbiome imbalance with cancer development is being one of the research hotspots.
32656096	4	66	theme	validation	928:937	arg1	cohort					939:944	validation cohort	928:944	validation cohort of 45 HPV16 positive women and 53 controls	928:987	We further employed qPCR assays to evaluate two selected gene markers of HPV16 infection in an independent validation cohort consisting of 88 HPV16 positive women and 81 controls, and six selected species markers in a subset of validation cohort of 45 HPV16 positive women and 53 controls.
32656096	9	67	theme	fundamental	1960:1970	arg1	fact					1972:1975	a fundamental fact that there are altered composition and function of the vaginal microbiome in HPV16-positive women, suggesting that vaginal dysbiosis may be associated with HPV infection in the female genital tract	1958:2173	a fundamental fact that there are altered composition and function of the vaginal microbiome in HPV16-positive women, suggesting that vaginal dysbiosis may be associated with HPV infection in the female genital tract	1958:2173	These results highlight a fundamental fact that there are altered composition and function of the vaginal microbiome in HPV16-positive women, suggesting that vaginal dysbiosis may be associated with HPV infection in the female genital tract.
32656096	5	68	theme	Fusobacteria	1077:1088	arg1	higher					1127:1132	higher	1127:1132	higher	1127:1132	We found that the relative abundance of dominant Firmicutes was lower, Actinobacteria, Fusobacteria and viruses phyla were significantly higher in the HPV16-positive group; 77 genera including Gardnerella, Peptostreptococcus, and Prevotella were higher, and 20 genera including Lactobacillus and Aerococcus were lower in the HPV16-positive women.
32656096	5	68	theme	Fusobacteria	1077:1088	arg1	abundance					1017:1025	the relative abundance	1004:1025	the relative abundance of dominant Firmicutes was lower, Actinobacteria, Fusobacteria and viruses phyla	1004:1106	We found that the relative abundance of dominant Firmicutes was lower, Actinobacteria, Fusobacteria and viruses phyla were significantly higher in the HPV16-positive group; 77 genera including Gardnerella, Peptostreptococcus, and Prevotella were higher, and 20 genera including Lactobacillus and Aerococcus were lower in the HPV16-positive women.
32656096	5	69	theme	viruses	1094:1100	arg1	higher					1127:1132	higher	1127:1132	higher	1127:1132	We found that the relative abundance of dominant Firmicutes was lower, Actinobacteria, Fusobacteria and viruses phyla were significantly higher in the HPV16-positive group; 77 genera including Gardnerella, Peptostreptococcus, and Prevotella were higher, and 20 genera including Lactobacillus and Aerococcus were lower in the HPV16-positive women.
32656096	5	69	theme	viruses	1094:1100	arg1	abundance					1017:1025	the relative abundance	1004:1025	the relative abundance of dominant Firmicutes was lower, Actinobacteria, Fusobacteria and viruses phyla	1004:1106	We found that the relative abundance of dominant Firmicutes was lower, Actinobacteria, Fusobacteria and viruses phyla were significantly higher in the HPV16-positive group; 77 genera including Gardnerella, Peptostreptococcus, and Prevotella were higher, and 20 genera including Lactobacillus and Aerococcus were lower in the HPV16-positive women.
32656096	3	70	theme	comprehensive	572:584	arg1	investigation					586:598	a comprehensive investigation	570:598	a comprehensive investigation describing the microbial abundances and enriched metabolic functions in cervicovaginal metagenomes	570:697	Here we identified the compositional and functional alterations on vaginal samples from 27 HPV16 positive women and 25 age-matched HPV negative controls using shotgun metagenomic sequencing, to provide a comprehensive investigation describing the microbial abundances and enriched metabolic functions in cervicovaginal metagenomes.
32656096	2	71	theme	cervical	255:262	arg1	initiation					271:280	cervical cancer initiation	255:280	cervical cancer initiation	255:280	Persistent HPV infection is a causal event in cervical cancer initiation, but, little is known about the microbiome composition and function in HPV infection.
32656096	1	72	theme	microbiome	125:134	arg1	imbalance					136:144	microbiome imbalance	125:144	microbiome imbalance	125:144	The association of microbiome imbalance with cancer development is being one of the research hotspots.
32656096	4	73	theme	selected	748:755	arg1	markers					762:768	two selected gene markers	744:768	two selected gene markers of HPV16 infection in an independent validation cohort consisting of 88 HPV16 positive women and 81 controls	744:877	We further employed qPCR assays to evaluate two selected gene markers of HPV16 infection in an independent validation cohort consisting of 88 HPV16 positive women and 81 controls, and six selected species markers in a subset of validation cohort of 45 HPV16 positive women and 53 controls.
32656096	3	74	theme	microbial	615:623	arg1	abundances					625:634	the microbial abundances	611:634	the microbial abundances	611:634	Here we identified the compositional and functional alterations on vaginal samples from 27 HPV16 positive women and 25 age-matched HPV negative controls using shotgun metagenomic sequencing, to provide a comprehensive investigation describing the microbial abundances and enriched metabolic functions in cervicovaginal metagenomes.
32656096	5	75	dep	Peptostreptococcus	1196:1213	arg1	Gardnerella					1183:1193	Gardnerella, Peptostreptococcus, and Prevotella	1183:1229	Gardnerella	1183:1193	We found that the relative abundance of dominant Firmicutes was lower, Actinobacteria, Fusobacteria and viruses phyla were significantly higher in the HPV16-positive group; 77 genera including Gardnerella, Peptostreptococcus, and Prevotella were higher, and 20 genera including Lactobacillus and Aerococcus were lower in the HPV16-positive women.
32656096	0	76	theme	Shotgun	74:80	arg1	Sequencing					94:103	Shotgun Metagenomic Sequencing	74:103	Shotgun Metagenomic Sequencing	74:103	The Alterations of Vaginal Microbiome in HPV16 Infection as Identified by Shotgun Metagenomic Sequencing.
32656096	4	77	from	cohort	818:823	arg1	markers					762:768	two selected gene markers	744:768	two selected gene markers of HPV16 infection in an independent validation cohort consisting of 88 HPV16 positive women and 81 controls	744:877	We further employed qPCR assays to evaluate two selected gene markers of HPV16 infection in an independent validation cohort consisting of 88 HPV16 positive women and 81 controls, and six selected species markers in a subset of validation cohort of 45 HPV16 positive women and 53 controls.
32656096	4	77	from	cohort	818:823	arg1	markers					905:911	six selected species markers	884:911	six selected species markers in a subset of validation cohort of 45 HPV16 positive women and 53 controls	884:987	We further employed qPCR assays to evaluate two selected gene markers of HPV16 infection in an independent validation cohort consisting of 88 HPV16 positive women and 81 controls, and six selected species markers in a subset of validation cohort of 45 HPV16 positive women and 53 controls.
32656096	0	78	from	Alterations	4:14	arg1	Infection					47:55	HPV16 Infection	41:55	HPV16 Infection	41:55	The Alterations of Vaginal Microbiome in HPV16 Infection as Identified by Shotgun Metagenomic Sequencing.
32656096	8	79	theme	Quantitative	1802:1813	arg1	measurements					1819:1830	Quantitative PCR measurements	1802:1830	Quantitative PCR measurements	1802:1830	Quantitative PCR measurements validated that one gene marker and three species were significantly enriched in HPV16-positive women.
32656096	9	80	theme	microbiome	2040:2049	arg1	function					2016:2023	function	2016:2023	function	2016:2023	These results highlight a fundamental fact that there are altered composition and function of the vaginal microbiome in HPV16-positive women, suggesting that vaginal dysbiosis may be associated with HPV infection in the female genital tract.
32656096	9	80	theme	microbiome	2040:2049	arg1	composition					2000:2010	altered composition	1992:2010	altered composition	1992:2010	These results highlight a fundamental fact that there are altered composition and function of the vaginal microbiome in HPV16-positive women, suggesting that vaginal dysbiosis may be associated with HPV infection in the female genital tract.
32656096	6	81	theme	genera	1363:1368	arg1	Abundance					1337:1345	Abundance	1337:1345	Abundance of 12 genes, 17 genera, and 7 species biomarkers	1337:1394	Abundance of 12 genes, 17 genera, and 7 species biomarkers showed an excellent predictive power for the HPV16-positive individuals, with 0.861, 0.819, and 0.918, respectively, of the area under the receiver-operating characteristic curve (AUC).
32656096	9	82	from	function	2016:2023	arg1	women					2069:2073	HPV16-positive women	2054:2073	HPV16-positive women	2054:2073	These results highlight a fundamental fact that there are altered composition and function of the vaginal microbiome in HPV16-positive women, suggesting that vaginal dysbiosis may be associated with HPV infection in the female genital tract.
32656096	4	83	theme	women	967:971	arg1	cohort					939:944	validation cohort	928:944	validation cohort of 45 HPV16 positive women and 53 controls	928:987	We further employed qPCR assays to evaluate two selected gene markers of HPV16 infection in an independent validation cohort consisting of 88 HPV16 positive women and 81 controls, and six selected species markers in a subset of validation cohort of 45 HPV16 positive women and 53 controls.
32656096	9	84	theme	HPV16-positive	2054:2067	arg1	women					2069:2073	HPV16-positive women	2054:2073	HPV16-positive women	2054:2073	These results highlight a fundamental fact that there are altered composition and function of the vaginal microbiome in HPV16-positive women, suggesting that vaginal dysbiosis may be associated with HPV infection in the female genital tract.
32656096	4	85	theme	independent	795:805	arg1	cohort					818:823	an independent validation cohort	792:823	an independent validation cohort consisting of 88 HPV16 positive women and 81 controls	792:877	We further employed qPCR assays to evaluate two selected gene markers of HPV16 infection in an independent validation cohort consisting of 88 HPV16 positive women and 81 controls, and six selected species markers in a subset of validation cohort of 45 HPV16 positive women and 53 controls.
32656096	3	86	theme	vaginal	435:441	arg1	samples					443:449	vaginal samples	435:449	vaginal samples from 27 HPV16 positive women and 25 age-matched HPV negative controls	435:519	Here we identified the compositional and functional alterations on vaginal samples from 27 HPV16 positive women and 25 age-matched HPV negative controls using shotgun metagenomic sequencing, to provide a comprehensive investigation describing the microbial abundances and enriched metabolic functions in cervicovaginal metagenomes.
32656096	1	87	with	association	110:120	arg1	development					158:168	cancer development	151:168	cancer development	151:168	The association of microbiome imbalance with cancer development is being one of the research hotspots.
32656096	9	88	from	composition	2000:2010	arg1	women					2069:2073	HPV16-positive women	2054:2073	HPV16-positive women	2054:2073	These results highlight a fundamental fact that there are altered composition and function of the vaginal microbiome in HPV16-positive women, suggesting that vaginal dysbiosis may be associated with HPV infection in the female genital tract.
32656096	5	89	theme	HPV16-positive	1315:1328	arg1	women					1330:1334	the HPV16-positive women	1311:1334	the HPV16-positive women	1311:1334	We found that the relative abundance of dominant Firmicutes was lower, Actinobacteria, Fusobacteria and viruses phyla were significantly higher in the HPV16-positive group; 77 genera including Gardnerella, Peptostreptococcus, and Prevotella were higher, and 20 genera including Lactobacillus and Aerococcus were lower in the HPV16-positive women.
32656096	0	90	theme	Vaginal	19:25	arg1	Microbiome					27:36	Vaginal Microbiome	19:36	Vaginal Microbiome	19:36	The Alterations of Vaginal Microbiome in HPV16 Infection as Identified by Shotgun Metagenomic Sequencing.
32656096	4	91	from	markers	762:768	arg1	subset					918:923	a subset	916:923	a subset of validation cohort of 45 HPV16 positive women and 53 controls	916:987	We further employed qPCR assays to evaluate two selected gene markers of HPV16 infection in an independent validation cohort consisting of 88 HPV16 positive women and 81 controls, and six selected species markers in a subset of validation cohort of 45 HPV16 positive women and 53 controls.
32656096	4	91	from	markers	762:768	arg1	cohort					818:823	an independent validation cohort	792:823	an independent validation cohort consisting of 88 HPV16 positive women and 81 controls	792:877	We further employed qPCR assays to evaluate two selected gene markers of HPV16 infection in an independent validation cohort consisting of 88 HPV16 positive women and 81 controls, and six selected species markers in a subset of validation cohort of 45 HPV16 positive women and 53 controls.
32656096	4	92	theme	HPV16	773:777	arg1	infection					779:787	HPV16 infection	773:787	HPV16 infection in an independent validation cohort consisting of 88 HPV16 positive women and 81 controls	773:877	We further employed qPCR assays to evaluate two selected gene markers of HPV16 infection in an independent validation cohort consisting of 88 HPV16 positive women and 81 controls, and six selected species markers in a subset of validation cohort of 45 HPV16 positive women and 53 controls.
32656096	2	93	theme	HPV	353:355	arg1	infection					357:365	HPV infection	353:365	HPV infection	353:365	Persistent HPV infection is a causal event in cervical cancer initiation, but, little is known about the microbiome composition and function in HPV infection.
32656096	7	94	theme	HPV16-positive	1649:1662	arg1	women					1664:1668	HPV16-positive women	1649:1668	HPV16-positive women	1649:1668	We further characterized the microbial function, and revealed that HPV16-positive women were enriched in metabolism and membrane transport, and depleted by glycan biosynthesis and metabolism, and replication and repair.
32656096	3	95	theme	metabolic	649:657	arg1	functions					659:667	enriched metabolic functions	640:667	enriched metabolic functions in cervicovaginal metagenomes	640:697	Here we identified the compositional and functional alterations on vaginal samples from 27 HPV16 positive women and 25 age-matched HPV negative controls using shotgun metagenomic sequencing, to provide a comprehensive investigation describing the microbial abundances and enriched metabolic functions in cervicovaginal metagenomes.
32656096	6	96	theme	excellent	1406:1414	arg1	power					1427:1431	an excellent predictive power	1403:1431	an excellent predictive power for the HPV16-positive individuals	1403:1466	Abundance of 12 genes, 17 genera, and 7 species biomarkers showed an excellent predictive power for the HPV16-positive individuals, with 0.861, 0.819, and 0.918, respectively, of the area under the receiver-operating characteristic curve (AUC).
32656096	2	97	theme	Persistent	209:218	arg1	event					246:250	a causal event	237:250	a causal event in cervical cancer initiation	237:280	Persistent HPV infection is a causal event in cervical cancer initiation, but, little is known about the microbiome composition and function in HPV infection.
32656096	2	97	theme	Persistent	209:218	arg1	infection					224:232	Persistent HPV infection	209:232	Persistent HPV infection	209:232	Persistent HPV infection is a causal event in cervical cancer initiation, but, little is known about the microbiome composition and function in HPV infection.
32656096	2	98	theme	microbiome	314:323	arg1	composition					325:335	microbiome composition	314:335	microbiome composition	314:335	Persistent HPV infection is a causal event in cervical cancer initiation, but, little is known about the microbiome composition and function in HPV infection.
34144192	1	0	with	diseases	306:313	arg1	etiology					331:338	an unknown etiology	320:338	an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC)	320:421	INTRODUCTION Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), is a group of chronic recurrent and incurable gastrointestinal diseases with an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC).
34144192	3	1	from	effect	859:864	arg1	symptoms					894:901	the clinical symptoms	881:901	the clinical symptoms of colitis	881:912	METHODS DSS-induced colitis and colitis-associated carcinogenesis (CAC) model were treated with MELK inhibitor OTSSP167 then the fight against effect of OTSSP167 in the clinical symptoms of colitis and CAC was measured.
34144192	4	2	theme	response	1059:1066	arg1	effect					1068:1073	anti-inflammatory response effect	1041:1073	anti-inflammatory response effect	1041:1073	In addition, underlying mechanism of OTSSP167 treatment in vitro and vivo including anti-ferroptosis and anti-inflammatory response effect was further explored.
34144192	5	3	dep	occurrence	1297:1306	arg1	the					1293:1295	the	1293:1295	the	1293:1295	RESULTS We found that pharmacological inhibition of MELK was indicated to significantly alleviate the inflammatory response in mice with colitis, reduce intestinal damage, and effectively inhibit the occurrence and progression of colitis-propelled carcinogenesis, which was closely related to the regulation of gut microbial composition, and OTSSP167-mediated fecal microbiota transplantation effectively alleviated DSS-induced colitis.
34144192	5	4	from	response	1212:1219	arg1	mice					1224:1227	mice	1224:1227	mice with colitis	1224:1240	RESULTS We found that pharmacological inhibition of MELK was indicated to significantly alleviate the inflammatory response in mice with colitis, reduce intestinal damage, and effectively inhibit the occurrence and progression of colitis-propelled carcinogenesis, which was closely related to the regulation of gut microbial composition, and OTSSP167-mediated fecal microbiota transplantation effectively alleviated DSS-induced colitis.
34144192	1	5	theme	ulcerative	193:202	arg1	colitis					204:210	ulcerative colitis	193:210	ulcerative colitis (UC)	193:215	INTRODUCTION Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), is a group of chronic recurrent and incurable gastrointestinal diseases with an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC).
34144192	1	5	theme	ulcerative	193:202	arg1	UC					213:214	UC	213:214	UC	213:214	INTRODUCTION Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), is a group of chronic recurrent and incurable gastrointestinal diseases with an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC).
34144192	2	6	theme	MELK-selective	574:587	arg1	OTSSP167					562:569	OTSSP167	562:569	OTSSP167 (a MELK-selective inhibitor)	562:598	OBJECTIVES In this study, we measured the expression characteristics of MELK in IBD and CRC tissues and explored the regulatory effect of OTSSP167 (a MELK-selective inhibitor) on the mice models of colitis and colitis-associated carcinogenesis and analyzed the specific molecular mechanisms.
34144192	2	6	theme	MELK-selective	574:587	arg1	inhibitor					589:597	a MELK-selective inhibitor	572:597	a MELK-selective inhibitor	572:597	OBJECTIVES In this study, we measured the expression characteristics of MELK in IBD and CRC tissues and explored the regulatory effect of OTSSP167 (a MELK-selective inhibitor) on the mice models of colitis and colitis-associated carcinogenesis and analyzed the specific molecular mechanisms.
34144192	8	7	theme	theoretical	2011:2021	arg1	treatment					2063:2071	a treatment	2061:2071	a treatment for IBD	2061:2079	CONCLUSION Our findings lay a theoretical foundation for the use of OTSSP167 as a treatment for IBD and its inhibition of the occurrence of colitis-associated carcinogenesis; additionally, MELK may be a potentially effective target molecule, thus providing more options for clinical treatment.
34144192	8	7	theme	theoretical	2011:2021	arg1	foundation					2023:2032	a theoretical foundation	2009:2032	a theoretical foundation for the use of OTSSP167	2009:2056	CONCLUSION Our findings lay a theoretical foundation for the use of OTSSP167 as a treatment for IBD and its inhibition of the occurrence of colitis-associated carcinogenesis; additionally, MELK may be a potentially effective target molecule, thus providing more options for clinical treatment.
34144192	5	8	theme	fecal	1457:1461	arg1	transplantation					1474:1488	OTSSP167-mediated fecal microbiota transplantation	1439:1488	OTSSP167-mediated fecal microbiota transplantation	1439:1488	RESULTS We found that pharmacological inhibition of MELK was indicated to significantly alleviate the inflammatory response in mice with colitis, reduce intestinal damage, and effectively inhibit the occurrence and progression of colitis-propelled carcinogenesis, which was closely related to the regulation of gut microbial composition, and OTSSP167-mediated fecal microbiota transplantation effectively alleviated DSS-induced colitis.
34144192	1	9	theme	high	356:359	arg1	risk					361:364	a high risk	354:364	a high risk of developing colitis-associated colorectal cancer (CRC)	354:421	INTRODUCTION Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), is a group of chronic recurrent and incurable gastrointestinal diseases with an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC).
34144192	2	10	theme	regulatory	541:550	arg1	effect					552:557	the regulatory effect	537:557	the regulatory effect of OTSSP167 (a MELK-selective inhibitor) on the mice models of colitis and colitis-associated carcinogenesis	537:666	OBJECTIVES In this study, we measured the expression characteristics of MELK in IBD and CRC tissues and explored the regulatory effect of OTSSP167 (a MELK-selective inhibitor) on the mice models of colitis and colitis-associated carcinogenesis and analyzed the specific molecular mechanisms.
34144192	3	11	theme	colitis-associated	748:765	arg1	METHODS					716:722	METHODS	716:722	METHODS DSS-induced colitis and colitis-associated carcinogenesis (CAC) model	716:792	METHODS DSS-induced colitis and colitis-associated carcinogenesis (CAC) model were treated with MELK inhibitor OTSSP167 then the fight against effect of OTSSP167 in the clinical symptoms of colitis and CAC was measured.
34144192	3	11	theme	colitis-associated	748:765	arg1	model					788:792	colitis-associated carcinogenesis (CAC) model	748:792	colitis-associated carcinogenesis (CAC) model	748:792	METHODS DSS-induced colitis and colitis-associated carcinogenesis (CAC) model were treated with MELK inhibitor OTSSP167 then the fight against effect of OTSSP167 in the clinical symptoms of colitis and CAC was measured.
34144192	8	12	theme	OTSSP167	2049:2056	arg1	use					2042:2044	the use	2038:2044	the use of OTSSP167	2038:2056	CONCLUSION Our findings lay a theoretical foundation for the use of OTSSP167 as a treatment for IBD and its inhibition of the occurrence of colitis-associated carcinogenesis; additionally, MELK may be a potentially effective target molecule, thus providing more options for clinical treatment.
34144192	1	13	theme	colitis-associated	380:397	arg1	CRC					418:420	CRC	418:420	CRC	418:420	INTRODUCTION Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), is a group of chronic recurrent and incurable gastrointestinal diseases with an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC).
34144192	1	13	theme	colitis-associated	380:397	arg1	cancer					410:415	colitis-associated colorectal cancer	380:415	colitis-associated colorectal cancer (CRC)	380:421	INTRODUCTION Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), is a group of chronic recurrent and incurable gastrointestinal diseases with an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC).
34144192	1	14	theme	Inflammatory	149:160	arg1	IBD					177:179	IBD	177:179	IBD	177:179	INTRODUCTION Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), is a group of chronic recurrent and incurable gastrointestinal diseases with an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC).
34144192	1	14	theme	Inflammatory	149:160	arg1	disease					168:174	Inflammatory bowel disease	149:174	INTRODUCTION Inflammatory bowel disease (IBD)	136:180	INTRODUCTION Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), is a group of chronic recurrent and incurable gastrointestinal diseases with an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC).
34144192	2	15	theme	carcinogenesis	653:666	arg1	models					612:617	the mice models	603:617	the mice models of colitis and colitis-associated carcinogenesis	603:666	OBJECTIVES In this study, we measured the expression characteristics of MELK in IBD and CRC tissues and explored the regulatory effect of OTSSP167 (a MELK-selective inhibitor) on the mice models of colitis and colitis-associated carcinogenesis and analyzed the specific molecular mechanisms.
34144192	7	16	dep	inhibited	1810:1818	arg1	control					1947:1953	control	1947:1953	control the occurrence of cancer	1947:1978	Further exploration of the molecular mechanism revealed that OTSSP167 inhibited AKT/IKK/P65 and ERK/IKK/P65 signaling cascades both in vivo and in vitro, which may help alleviate intestinal inflammation and control the occurrence of cancer.
34144192	7	16	dep	inhibited	1810:1818	arg1	help					1904:1907	help	1904:1907	help	1904:1907	Further exploration of the molecular mechanism revealed that OTSSP167 inhibited AKT/IKK/P65 and ERK/IKK/P65 signaling cascades both in vivo and in vitro, which may help alleviate intestinal inflammation and control the occurrence of cancer.
34144192	6	17	theme	M1	1666:1667	arg1	polarization					1669:1680	M1 polarization	1666:1680	M1 polarization	1666:1680	In addition, OTSSP167 treatment obviously inhibited ferroptosis in the intestinal tissue and suppressed macrophage infiltration and M1 polarization, which reduced the secretion of pro-inflammatory factors.
34144192	4	18	theme	underlying	949:958	arg1	mechanism					960:968	underlying mechanism	949:968	underlying mechanism of OTSSP167 treatment in vitro and vivo including anti-ferroptosis and anti-inflammatory response effect	949:1073	In addition, underlying mechanism of OTSSP167 treatment in vitro and vivo including anti-ferroptosis and anti-inflammatory response effect was further explored.
34144192	0	19	theme	inflammatory	65:76	arg1	response					78:85	the inflammatory response	61:85	the inflammatory response	61:85	Pharmacological inhibition of MELK restricts ferroptosis and the inflammatory response in colitis and colitis-propelled carcinogenesis.
34144192	5	20	theme	pharmacological	1119:1133	arg1	inhibition					1135:1144	pharmacological inhibition	1119:1144	pharmacological inhibition of MELK	1119:1152	RESULTS We found that pharmacological inhibition of MELK was indicated to significantly alleviate the inflammatory response in mice with colitis, reduce intestinal damage, and effectively inhibit the occurrence and progression of colitis-propelled carcinogenesis, which was closely related to the regulation of gut microbial composition, and OTSSP167-mediated fecal microbiota transplantation effectively alleviated DSS-induced colitis.
34144192	7	21	theme	ERK/IKK/P65	1836:1846	arg1	cascades					1858:1865	ERK/IKK/P65 signaling cascades	1836:1865	ERK/IKK/P65 signaling cascades	1836:1865	Further exploration of the molecular mechanism revealed that OTSSP167 inhibited AKT/IKK/P65 and ERK/IKK/P65 signaling cascades both in vivo and in vitro, which may help alleviate intestinal inflammation and control the occurrence of cancer.
34144192	1	22	theme	chronic	257:263	arg1	diseases					306:313	chronic recurrent and incurable gastrointestinal diseases	257:313	chronic recurrent and incurable gastrointestinal diseases with an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC)	257:421	INTRODUCTION Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), is a group of chronic recurrent and incurable gastrointestinal diseases with an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC).
34144192	2	23	theme	specific	685:692	arg1	mechanisms					704:713	the specific molecular mechanisms	681:713	the specific molecular mechanisms	681:713	OBJECTIVES In this study, we measured the expression characteristics of MELK in IBD and CRC tissues and explored the regulatory effect of OTSSP167 (a MELK-selective inhibitor) on the mice models of colitis and colitis-associated carcinogenesis and analyzed the specific molecular mechanisms.
34144192	3	24	theme	inhibitor	817:825	arg1	OTSSP167					827:834	MELK inhibitor OTSSP167	812:834	MELK inhibitor OTSSP167	812:834	METHODS DSS-induced colitis and colitis-associated carcinogenesis (CAC) model were treated with MELK inhibitor OTSSP167 then the fight against effect of OTSSP167 in the clinical symptoms of colitis and CAC was measured.
34144192	1	25	theme	unknown	323:329	arg1	etiology					331:338	an unknown etiology	320:338	an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC)	320:421	INTRODUCTION Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), is a group of chronic recurrent and incurable gastrointestinal diseases with an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC).
34144192	2	26	theme	mice	607:610	arg1	models					612:617	the mice models	603:617	the mice models of colitis and colitis-associated carcinogenesis	603:666	OBJECTIVES In this study, we measured the expression characteristics of MELK in IBD and CRC tissues and explored the regulatory effect of OTSSP167 (a MELK-selective inhibitor) on the mice models of colitis and colitis-associated carcinogenesis and analyzed the specific molecular mechanisms.
34144192	8	27	dep	CONCLUSION	1981:1990	arg1	lay					2005:2007	lay	2005:2007	lay a theoretical foundation for the use of OTSSP167 as a treatment for IBD and its inhibition of the occurrence of colitis-associated carcinogenesis	2005:2153	CONCLUSION Our findings lay a theoretical foundation for the use of OTSSP167 as a treatment for IBD and its inhibition of the occurrence of colitis-associated carcinogenesis; additionally, MELK may be a potentially effective target molecule, thus providing more options for clinical treatment.
34144192	1	28	dep	INTRODUCTION	136:147	arg1	IBD					177:179	IBD	177:179	IBD	177:179	INTRODUCTION Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), is a group of chronic recurrent and incurable gastrointestinal diseases with an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC).
34144192	1	28	dep	INTRODUCTION	136:147	arg1	disease					168:174	Inflammatory bowel disease	149:174	INTRODUCTION Inflammatory bowel disease (IBD)	136:180	INTRODUCTION Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), is a group of chronic recurrent and incurable gastrointestinal diseases with an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC).
34144192	6	29	theme	pro-inflammatory	1714:1729	arg1	factors					1731:1737	pro-inflammatory factors	1714:1737	pro-inflammatory factors	1714:1737	In addition, OTSSP167 treatment obviously inhibited ferroptosis in the intestinal tissue and suppressed macrophage infiltration and M1 polarization, which reduced the secretion of pro-inflammatory factors.
34144192	2	30	theme	expression	466:475	arg1	characteristics					477:491	the expression characteristics	462:491	the expression characteristics of MELK	462:499	OBJECTIVES In this study, we measured the expression characteristics of MELK in IBD and CRC tissues and explored the regulatory effect of OTSSP167 (a MELK-selective inhibitor) on the mice models of colitis and colitis-associated carcinogenesis and analyzed the specific molecular mechanisms.
34144192	0	31	theme	Pharmacological	0:14	arg1	inhibition					16:25	Pharmacological inhibition	0:25	Pharmacological inhibition of MELK	0:33	Pharmacological inhibition of MELK restricts ferroptosis and the inflammatory response in colitis and colitis-propelled carcinogenesis.
34144192	7	32	theme	molecular	1767:1775	arg1	mechanism					1777:1785	the molecular mechanism	1763:1785	the molecular mechanism	1763:1785	Further exploration of the molecular mechanism revealed that OTSSP167 inhibited AKT/IKK/P65 and ERK/IKK/P65 signaling cascades both in vivo and in vitro, which may help alleviate intestinal inflammation and control the occurrence of cancer.
34144192	2	33	from	effect	552:557	arg1	models					612:617	the mice models	603:617	the mice models of colitis and colitis-associated carcinogenesis	603:666	OBJECTIVES In this study, we measured the expression characteristics of MELK in IBD and CRC tissues and explored the regulatory effect of OTSSP167 (a MELK-selective inhibitor) on the mice models of colitis and colitis-associated carcinogenesis and analyzed the specific molecular mechanisms.
34144192	5	34	theme	colitis-propelled	1327:1343	arg1	carcinogenesis					1345:1358	colitis-propelled carcinogenesis	1327:1358	colitis-propelled carcinogenesis	1327:1358	RESULTS We found that pharmacological inhibition of MELK was indicated to significantly alleviate the inflammatory response in mice with colitis, reduce intestinal damage, and effectively inhibit the occurrence and progression of colitis-propelled carcinogenesis, which was closely related to the regulation of gut microbial composition, and OTSSP167-mediated fecal microbiota transplantation effectively alleviated DSS-induced colitis.
34144192	8	35	theme	carcinogenesis	2140:2153	arg1	occurrence					2107:2116	the occurrence	2103:2116	the occurrence of colitis-associated carcinogenesis	2103:2153	CONCLUSION Our findings lay a theoretical foundation for the use of OTSSP167 as a treatment for IBD and its inhibition of the occurrence of colitis-associated carcinogenesis; additionally, MELK may be a potentially effective target molecule, thus providing more options for clinical treatment.
34144192	5	36	theme	inflammatory	1199:1210	arg1	response					1212:1219	the inflammatory response	1195:1219	the inflammatory response in mice with colitis	1195:1240	RESULTS We found that pharmacological inhibition of MELK was indicated to significantly alleviate the inflammatory response in mice with colitis, reduce intestinal damage, and effectively inhibit the occurrence and progression of colitis-propelled carcinogenesis, which was closely related to the regulation of gut microbial composition, and OTSSP167-mediated fecal microbiota transplantation effectively alleviated DSS-induced colitis.
34144192	3	37	theme	colitis	906:912	arg1	symptoms					894:901	the clinical symptoms	881:901	the clinical symptoms of colitis	881:912	METHODS DSS-induced colitis and colitis-associated carcinogenesis (CAC) model were treated with MELK inhibitor OTSSP167 then the fight against effect of OTSSP167 in the clinical symptoms of colitis and CAC was measured.
34144192	8	38	theme	target	2206:2211	arg1	molecule					2213:2220	a potentially effective target molecule	2182:2220	a potentially effective target molecule	2182:2220	CONCLUSION Our findings lay a theoretical foundation for the use of OTSSP167 as a treatment for IBD and its inhibition of the occurrence of colitis-associated carcinogenesis; additionally, MELK may be a potentially effective target molecule, thus providing more options for clinical treatment.
34144192	8	38	theme	target	2206:2211	arg1	MELK					2170:2173	MELK	2170:2173	MELK	2170:2173	CONCLUSION Our findings lay a theoretical foundation for the use of OTSSP167 as a treatment for IBD and its inhibition of the occurrence of colitis-associated carcinogenesis; additionally, MELK may be a potentially effective target molecule, thus providing more options for clinical treatment.
34144192	4	39	theme	treatment	982:990	arg1	mechanism					960:968	underlying mechanism	949:968	underlying mechanism of OTSSP167 treatment in vitro and vivo including anti-ferroptosis and anti-inflammatory response effect	949:1073	In addition, underlying mechanism of OTSSP167 treatment in vitro and vivo including anti-ferroptosis and anti-inflammatory response effect was further explored.
34144192	5	40	theme	microbial	1412:1420	arg1	composition					1422:1432	gut microbial composition	1408:1432	gut microbial composition	1408:1432	RESULTS We found that pharmacological inhibition of MELK was indicated to significantly alleviate the inflammatory response in mice with colitis, reduce intestinal damage, and effectively inhibit the occurrence and progression of colitis-propelled carcinogenesis, which was closely related to the regulation of gut microbial composition, and OTSSP167-mediated fecal microbiota transplantation effectively alleviated DSS-induced colitis.
34144192	1	41	theme	gastrointestinal	289:304	arg1	diseases					306:313	chronic recurrent and incurable gastrointestinal diseases	257:313	chronic recurrent and incurable gastrointestinal diseases with an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC)	257:421	INTRODUCTION Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), is a group of chronic recurrent and incurable gastrointestinal diseases with an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC).
34144192	3	42	dep	METHODS	716:722	arg1	colitis					736:742	DSS-induced colitis	724:742	DSS-induced colitis	724:742	METHODS DSS-induced colitis and colitis-associated carcinogenesis (CAC) model were treated with MELK inhibitor OTSSP167 then the fight against effect of OTSSP167 in the clinical symptoms of colitis and CAC was measured.
34144192	3	42	dep	METHODS	716:722	arg1	METHODS					716:722	METHODS	716:722	METHODS DSS-induced colitis and colitis-associated carcinogenesis (CAC) model	716:792	METHODS DSS-induced colitis and colitis-associated carcinogenesis (CAC) model were treated with MELK inhibitor OTSSP167 then the fight against effect of OTSSP167 in the clinical symptoms of colitis and CAC was measured.
34144192	3	42	dep	METHODS	716:722	arg1	model					788:792	colitis-associated carcinogenesis (CAC) model	748:792	colitis-associated carcinogenesis (CAC) model	748:792	METHODS DSS-induced colitis and colitis-associated carcinogenesis (CAC) model were treated with MELK inhibitor OTSSP167 then the fight against effect of OTSSP167 in the clinical symptoms of colitis and CAC was measured.
34144192	5	43	with	mice	1224:1227	arg1	colitis					1234:1240	colitis	1234:1240	colitis	1234:1240	RESULTS We found that pharmacological inhibition of MELK was indicated to significantly alleviate the inflammatory response in mice with colitis, reduce intestinal damage, and effectively inhibit the occurrence and progression of colitis-propelled carcinogenesis, which was closely related to the regulation of gut microbial composition, and OTSSP167-mediated fecal microbiota transplantation effectively alleviated DSS-induced colitis.
34144192	3	44	theme	OTSSP167	869:876	arg1	effect					859:864	effect	859:864	effect of OTSSP167 in the clinical symptoms of colitis	859:912	METHODS DSS-induced colitis and colitis-associated carcinogenesis (CAC) model were treated with MELK inhibitor OTSSP167 then the fight against effect of OTSSP167 in the clinical symptoms of colitis and CAC was measured.
34144192	5	45	theme	intestinal	1250:1259	arg1	damage					1261:1266	intestinal damage	1250:1266	intestinal damage	1250:1266	RESULTS We found that pharmacological inhibition of MELK was indicated to significantly alleviate the inflammatory response in mice with colitis, reduce intestinal damage, and effectively inhibit the occurrence and progression of colitis-propelled carcinogenesis, which was closely related to the regulation of gut microbial composition, and OTSSP167-mediated fecal microbiota transplantation effectively alleviated DSS-induced colitis.
34144192	7	46	theme	intestinal	1919:1928	arg1	inflammation					1930:1941	intestinal inflammation	1919:1941	intestinal inflammation	1919:1941	Further exploration of the molecular mechanism revealed that OTSSP167 inhibited AKT/IKK/P65 and ERK/IKK/P65 signaling cascades both in vivo and in vitro, which may help alleviate intestinal inflammation and control the occurrence of cancer.
34144192	8	47	theme	clinical	2255:2262	arg1	treatment					2264:2272	clinical treatment	2255:2272	clinical treatment	2255:2272	CONCLUSION Our findings lay a theoretical foundation for the use of OTSSP167 as a treatment for IBD and its inhibition of the occurrence of colitis-associated carcinogenesis; additionally, MELK may be a potentially effective target molecule, thus providing more options for clinical treatment.
34144192	4	48	theme	anti-inflammatory	1041:1057	arg1	effect					1068:1073	anti-inflammatory response effect	1041:1073	anti-inflammatory response effect	1041:1073	In addition, underlying mechanism of OTSSP167 treatment in vitro and vivo including anti-ferroptosis and anti-inflammatory response effect was further explored.
34144192	2	49	theme	OTSSP167	562:569	arg1	effect					552:557	the regulatory effect	537:557	the regulatory effect of OTSSP167 (a MELK-selective inhibitor) on the mice models of colitis and colitis-associated carcinogenesis	537:666	OBJECTIVES In this study, we measured the expression characteristics of MELK in IBD and CRC tissues and explored the regulatory effect of OTSSP167 (a MELK-selective inhibitor) on the mice models of colitis and colitis-associated carcinogenesis and analyzed the specific molecular mechanisms.
34144192	2	50	dep	IBD	504:506	arg1	tissues					516:522	tissues	516:522	tissues	516:522	OBJECTIVES In this study, we measured the expression characteristics of MELK in IBD and CRC tissues and explored the regulatory effect of OTSSP167 (a MELK-selective inhibitor) on the mice models of colitis and colitis-associated carcinogenesis and analyzed the specific molecular mechanisms.
34144192	5	51	theme	DSS-induced	1513:1523	arg1	colitis					1525:1531	DSS-induced colitis	1513:1531	DSS-induced colitis	1513:1531	RESULTS We found that pharmacological inhibition of MELK was indicated to significantly alleviate the inflammatory response in mice with colitis, reduce intestinal damage, and effectively inhibit the occurrence and progression of colitis-propelled carcinogenesis, which was closely related to the regulation of gut microbial composition, and OTSSP167-mediated fecal microbiota transplantation effectively alleviated DSS-induced colitis.
34144192	5	52	theme	OTSSP167-mediated	1439:1455	arg1	transplantation					1474:1488	OTSSP167-mediated fecal microbiota transplantation	1439:1488	OTSSP167-mediated fecal microbiota transplantation	1439:1488	RESULTS We found that pharmacological inhibition of MELK was indicated to significantly alleviate the inflammatory response in mice with colitis, reduce intestinal damage, and effectively inhibit the occurrence and progression of colitis-propelled carcinogenesis, which was closely related to the regulation of gut microbial composition, and OTSSP167-mediated fecal microbiota transplantation effectively alleviated DSS-induced colitis.
34144192	6	53	theme	intestinal	1605:1614	arg1	tissue					1616:1621	the intestinal tissue	1601:1621	the intestinal tissue	1601:1621	In addition, OTSSP167 treatment obviously inhibited ferroptosis in the intestinal tissue and suppressed macrophage infiltration and M1 polarization, which reduced the secretion of pro-inflammatory factors.
34144192	7	54	theme	Further	1740:1746	arg1	exploration					1748:1758	Further exploration	1740:1758	Further exploration of the molecular mechanism	1740:1785	Further exploration of the molecular mechanism revealed that OTSSP167 inhibited AKT/IKK/P65 and ERK/IKK/P65 signaling cascades both in vivo and in vitro, which may help alleviate intestinal inflammation and control the occurrence of cancer.
34144192	5	55	theme	microbiota	1463:1472	arg1	transplantation					1474:1488	OTSSP167-mediated fecal microbiota transplantation	1439:1488	OTSSP167-mediated fecal microbiota transplantation	1439:1488	RESULTS We found that pharmacological inhibition of MELK was indicated to significantly alleviate the inflammatory response in mice with colitis, reduce intestinal damage, and effectively inhibit the occurrence and progression of colitis-propelled carcinogenesis, which was closely related to the regulation of gut microbial composition, and OTSSP167-mediated fecal microbiota transplantation effectively alleviated DSS-induced colitis.
34144192	3	56	theme	DSS-induced	724:734	arg1	colitis					736:742	DSS-induced colitis	724:742	DSS-induced colitis	724:742	METHODS DSS-induced colitis and colitis-associated carcinogenesis (CAC) model were treated with MELK inhibitor OTSSP167 then the fight against effect of OTSSP167 in the clinical symptoms of colitis and CAC was measured.
34144192	3	56	theme	DSS-induced	724:734	arg1	METHODS					716:722	METHODS	716:722	METHODS DSS-induced colitis and colitis-associated carcinogenesis (CAC) model	716:792	METHODS DSS-induced colitis and colitis-associated carcinogenesis (CAC) model were treated with MELK inhibitor OTSSP167 then the fight against effect of OTSSP167 in the clinical symptoms of colitis and CAC was measured.
34144192	0	57	from	response	78:85	arg1	colitis					90:96	colitis	90:96	colitis	90:96	Pharmacological inhibition of MELK restricts ferroptosis and the inflammatory response in colitis and colitis-propelled carcinogenesis.
34144192	0	57	from	response	78:85	arg1	carcinogenesis					120:133	colitis-propelled carcinogenesis	102:133	colitis-propelled carcinogenesis	102:133	Pharmacological inhibition of MELK restricts ferroptosis and the inflammatory response in colitis and colitis-propelled carcinogenesis.
34144192	3	58	theme	CAC	783:785	arg1	METHODS					716:722	METHODS	716:722	METHODS DSS-induced colitis and colitis-associated carcinogenesis (CAC) model	716:792	METHODS DSS-induced colitis and colitis-associated carcinogenesis (CAC) model were treated with MELK inhibitor OTSSP167 then the fight against effect of OTSSP167 in the clinical symptoms of colitis and CAC was measured.
34144192	3	58	theme	CAC	783:785	arg1	model					788:792	colitis-associated carcinogenesis (CAC) model	748:792	colitis-associated carcinogenesis (CAC) model	748:792	METHODS DSS-induced colitis and colitis-associated carcinogenesis (CAC) model were treated with MELK inhibitor OTSSP167 then the fight against effect of OTSSP167 in the clinical symptoms of colitis and CAC was measured.
34144192	3	59	theme	carcinogenesis	767:780	arg1	METHODS					716:722	METHODS	716:722	METHODS DSS-induced colitis and colitis-associated carcinogenesis (CAC) model	716:792	METHODS DSS-induced colitis and colitis-associated carcinogenesis (CAC) model were treated with MELK inhibitor OTSSP167 then the fight against effect of OTSSP167 in the clinical symptoms of colitis and CAC was measured.
34144192	3	59	theme	carcinogenesis	767:780	arg1	model					788:792	colitis-associated carcinogenesis (CAC) model	748:792	colitis-associated carcinogenesis (CAC) model	748:792	METHODS DSS-induced colitis and colitis-associated carcinogenesis (CAC) model were treated with MELK inhibitor OTSSP167 then the fight against effect of OTSSP167 in the clinical symptoms of colitis and CAC was measured.
34144192	1	60	theme	colorectal	399:408	arg1	CRC					418:420	CRC	418:420	CRC	418:420	INTRODUCTION Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), is a group of chronic recurrent and incurable gastrointestinal diseases with an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC).
34144192	1	60	theme	colorectal	399:408	arg1	cancer					410:415	colitis-associated colorectal cancer	380:415	colitis-associated colorectal cancer (CRC)	380:421	INTRODUCTION Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), is a group of chronic recurrent and incurable gastrointestinal diseases with an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC).
34144192	1	61	theme	bowel	162:166	arg1	IBD					177:179	IBD	177:179	IBD	177:179	INTRODUCTION Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), is a group of chronic recurrent and incurable gastrointestinal diseases with an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC).
34144192	1	61	theme	bowel	162:166	arg1	disease					168:174	Inflammatory bowel disease	149:174	INTRODUCTION Inflammatory bowel disease (IBD)	136:180	INTRODUCTION Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), is a group of chronic recurrent and incurable gastrointestinal diseases with an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC).
34144192	2	62	theme	molecular	694:702	arg1	mechanisms					704:713	the specific molecular mechanisms	681:713	the specific molecular mechanisms	681:713	OBJECTIVES In this study, we measured the expression characteristics of MELK in IBD and CRC tissues and explored the regulatory effect of OTSSP167 (a MELK-selective inhibitor) on the mice models of colitis and colitis-associated carcinogenesis and analyzed the specific molecular mechanisms.
34144192	6	63	theme	macrophage	1638:1647	arg1	infiltration					1649:1660	macrophage infiltration	1638:1660	macrophage infiltration	1638:1660	In addition, OTSSP167 treatment obviously inhibited ferroptosis in the intestinal tissue and suppressed macrophage infiltration and M1 polarization, which reduced the secretion of pro-inflammatory factors.
34144192	7	64	theme	signaling	1848:1856	arg1	cascades					1858:1865	ERK/IKK/P65 signaling cascades	1836:1865	ERK/IKK/P65 signaling cascades	1836:1865	Further exploration of the molecular mechanism revealed that OTSSP167 inhibited AKT/IKK/P65 and ERK/IKK/P65 signaling cascades both in vivo and in vitro, which may help alleviate intestinal inflammation and control the occurrence of cancer.
34144192	0	65	theme	colitis-propelled	102:118	arg1	carcinogenesis					120:133	colitis-propelled carcinogenesis	102:133	colitis-propelled carcinogenesis	102:133	Pharmacological inhibition of MELK restricts ferroptosis and the inflammatory response in colitis and colitis-propelled carcinogenesis.
34144192	5	66	theme	MELK	1149:1152	arg1	inhibition					1135:1144	pharmacological inhibition	1119:1144	pharmacological inhibition of MELK	1119:1152	RESULTS We found that pharmacological inhibition of MELK was indicated to significantly alleviate the inflammatory response in mice with colitis, reduce intestinal damage, and effectively inhibit the occurrence and progression of colitis-propelled carcinogenesis, which was closely related to the regulation of gut microbial composition, and OTSSP167-mediated fecal microbiota transplantation effectively alleviated DSS-induced colitis.
34144192	2	67	theme	MELK	496:499	arg1	characteristics					477:491	the expression characteristics	462:491	the expression characteristics of MELK	462:499	OBJECTIVES In this study, we measured the expression characteristics of MELK in IBD and CRC tissues and explored the regulatory effect of OTSSP167 (a MELK-selective inhibitor) on the mice models of colitis and colitis-associated carcinogenesis and analyzed the specific molecular mechanisms.
34144192	3	68	theme	MELK	812:815	arg1	OTSSP167					827:834	MELK inhibitor OTSSP167	812:834	MELK inhibitor OTSSP167	812:834	METHODS DSS-induced colitis and colitis-associated carcinogenesis (CAC) model were treated with MELK inhibitor OTSSP167 then the fight against effect of OTSSP167 in the clinical symptoms of colitis and CAC was measured.
34144192	8	69	theme	occurrence	2107:2116	arg1	foundation					2023:2032	a theoretical foundation	2009:2032	a theoretical foundation for the use of OTSSP167	2009:2056	CONCLUSION Our findings lay a theoretical foundation for the use of OTSSP167 as a treatment for IBD and its inhibition of the occurrence of colitis-associated carcinogenesis; additionally, MELK may be a potentially effective target molecule, thus providing more options for clinical treatment.
34144192	8	69	theme	occurrence	2107:2116	arg1	inhibition					2089:2098	its inhibition	2085:2098	its inhibition of the occurrence of colitis-associated carcinogenesis	2085:2153	CONCLUSION Our findings lay a theoretical foundation for the use of OTSSP167 as a treatment for IBD and its inhibition of the occurrence of colitis-associated carcinogenesis; additionally, MELK may be a potentially effective target molecule, thus providing more options for clinical treatment.
34144192	8	69	theme	occurrence	2107:2116	arg1	treatment					2063:2071	a treatment	2061:2071	a treatment for IBD	2061:2079	CONCLUSION Our findings lay a theoretical foundation for the use of OTSSP167 as a treatment for IBD and its inhibition of the occurrence of colitis-associated carcinogenesis; additionally, MELK may be a potentially effective target molecule, thus providing more options for clinical treatment.
34144192	6	70	theme	factors	1731:1737	arg1	secretion					1701:1709	the secretion	1697:1709	the secretion of pro-inflammatory factors	1697:1737	In addition, OTSSP167 treatment obviously inhibited ferroptosis in the intestinal tissue and suppressed macrophage infiltration and M1 polarization, which reduced the secretion of pro-inflammatory factors.
34144192	0	71	from	ferroptosis	45:55	arg1	colitis					90:96	colitis	90:96	colitis	90:96	Pharmacological inhibition of MELK restricts ferroptosis and the inflammatory response in colitis and colitis-propelled carcinogenesis.
34144192	0	71	from	ferroptosis	45:55	arg1	carcinogenesis					120:133	colitis-propelled carcinogenesis	102:133	colitis-propelled carcinogenesis	102:133	Pharmacological inhibition of MELK restricts ferroptosis and the inflammatory response in colitis and colitis-propelled carcinogenesis.
34144192	2	72	theme	colitis-associated	634:651	arg1	carcinogenesis					653:666	colitis-associated carcinogenesis	634:666	colitis-associated carcinogenesis	634:666	OBJECTIVES In this study, we measured the expression characteristics of MELK in IBD and CRC tissues and explored the regulatory effect of OTSSP167 (a MELK-selective inhibitor) on the mice models of colitis and colitis-associated carcinogenesis and analyzed the specific molecular mechanisms.
34144192	8	73	theme	colitis-associated	2121:2138	arg1	carcinogenesis					2140:2153	colitis-associated carcinogenesis	2121:2153	colitis-associated carcinogenesis	2121:2153	CONCLUSION Our findings lay a theoretical foundation for the use of OTSSP167 as a treatment for IBD and its inhibition of the occurrence of colitis-associated carcinogenesis; additionally, MELK may be a potentially effective target molecule, thus providing more options for clinical treatment.
34144192	2	74	dep	OBJECTIVES	424:433	arg1	analyzed					672:679	analyzed	672:679	analyzed the specific molecular mechanisms	672:713	OBJECTIVES In this study, we measured the expression characteristics of MELK in IBD and CRC tissues and explored the regulatory effect of OTSSP167 (a MELK-selective inhibitor) on the mice models of colitis and colitis-associated carcinogenesis and analyzed the specific molecular mechanisms.
34144192	2	74	dep	OBJECTIVES	424:433	arg1	explored					528:535	explored	528:535	explored the regulatory effect of OTSSP167 (a MELK-selective inhibitor) on the mice models of colitis and colitis-associated carcinogenesis	528:666	OBJECTIVES In this study, we measured the expression characteristics of MELK in IBD and CRC tissues and explored the regulatory effect of OTSSP167 (a MELK-selective inhibitor) on the mice models of colitis and colitis-associated carcinogenesis and analyzed the specific molecular mechanisms.
34144192	2	74	dep	OBJECTIVES	424:433	arg1	measured					453:460	measured	453:460	measured the expression characteristics of MELK in IBD and CRC tissues	453:522	OBJECTIVES In this study, we measured the expression characteristics of MELK in IBD and CRC tissues and explored the regulatory effect of OTSSP167 (a MELK-selective inhibitor) on the mice models of colitis and colitis-associated carcinogenesis and analyzed the specific molecular mechanisms.
34144192	6	75	from	ferroptosis	1586:1596	arg1	tissue					1616:1621	the intestinal tissue	1601:1621	the intestinal tissue	1601:1621	In addition, OTSSP167 treatment obviously inhibited ferroptosis in the intestinal tissue and suppressed macrophage infiltration and M1 polarization, which reduced the secretion of pro-inflammatory factors.
34144192	3	76	theme	clinical	885:892	arg1	symptoms					894:901	the clinical symptoms	881:901	the clinical symptoms of colitis	881:912	METHODS DSS-induced colitis and colitis-associated carcinogenesis (CAC) model were treated with MELK inhibitor OTSSP167 then the fight against effect of OTSSP167 in the clinical symptoms of colitis and CAC was measured.
34144192	8	77	theme	effective	2196:2204	arg1	molecule					2213:2220	a potentially effective target molecule	2182:2220	a potentially effective target molecule	2182:2220	CONCLUSION Our findings lay a theoretical foundation for the use of OTSSP167 as a treatment for IBD and its inhibition of the occurrence of colitis-associated carcinogenesis; additionally, MELK may be a potentially effective target molecule, thus providing more options for clinical treatment.
34144192	8	77	theme	effective	2196:2204	arg1	MELK					2170:2173	MELK	2170:2173	MELK	2170:2173	CONCLUSION Our findings lay a theoretical foundation for the use of OTSSP167 as a treatment for IBD and its inhibition of the occurrence of colitis-associated carcinogenesis; additionally, MELK may be a potentially effective target molecule, thus providing more options for clinical treatment.
34144192	2	78	theme	colitis	622:628	arg1	models					612:617	the mice models	603:617	the mice models of colitis and colitis-associated carcinogenesis	603:666	OBJECTIVES In this study, we measured the expression characteristics of MELK in IBD and CRC tissues and explored the regulatory effect of OTSSP167 (a MELK-selective inhibitor) on the mice models of colitis and colitis-associated carcinogenesis and analyzed the specific molecular mechanisms.
34144192	0	79	theme	MELK	30:33	arg1	inhibition					16:25	Pharmacological inhibition	0:25	Pharmacological inhibition of MELK	0:33	Pharmacological inhibition of MELK restricts ferroptosis and the inflammatory response in colitis and colitis-propelled carcinogenesis.
34144192	7	80	theme	mechanism	1777:1785	arg1	exploration					1748:1758	Further exploration	1740:1758	Further exploration of the molecular mechanism	1740:1785	Further exploration of the molecular mechanism revealed that OTSSP167 inhibited AKT/IKK/P65 and ERK/IKK/P65 signaling cascades both in vivo and in vitro, which may help alleviate intestinal inflammation and control the occurrence of cancer.
34144192	5	81	theme	carcinogenesis	1345:1358	arg1	occurrence					1297:1306	occurrence	1297:1306	occurrence	1297:1306	RESULTS We found that pharmacological inhibition of MELK was indicated to significantly alleviate the inflammatory response in mice with colitis, reduce intestinal damage, and effectively inhibit the occurrence and progression of colitis-propelled carcinogenesis, which was closely related to the regulation of gut microbial composition, and OTSSP167-mediated fecal microbiota transplantation effectively alleviated DSS-induced colitis.
34144192	5	81	theme	carcinogenesis	1345:1358	arg1	progression					1312:1322	progression	1312:1322	progression	1312:1322	RESULTS We found that pharmacological inhibition of MELK was indicated to significantly alleviate the inflammatory response in mice with colitis, reduce intestinal damage, and effectively inhibit the occurrence and progression of colitis-propelled carcinogenesis, which was closely related to the regulation of gut microbial composition, and OTSSP167-mediated fecal microbiota transplantation effectively alleviated DSS-induced colitis.
34144192	1	82	theme	recurrent	265:273	arg1	diseases					306:313	chronic recurrent and incurable gastrointestinal diseases	257:313	chronic recurrent and incurable gastrointestinal diseases with an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC)	257:421	INTRODUCTION Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), is a group of chronic recurrent and incurable gastrointestinal diseases with an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC).
34144192	4	83	theme	OTSSP167	973:980	arg1	treatment					982:990	OTSSP167 treatment	973:990	OTSSP167 treatment	973:990	In addition, underlying mechanism of OTSSP167 treatment in vitro and vivo including anti-ferroptosis and anti-inflammatory response effect was further explored.
34144192	5	84	theme	gut	1408:1410	arg1	composition					1422:1432	gut microbial composition	1408:1432	gut microbial composition	1408:1432	RESULTS We found that pharmacological inhibition of MELK was indicated to significantly alleviate the inflammatory response in mice with colitis, reduce intestinal damage, and effectively inhibit the occurrence and progression of colitis-propelled carcinogenesis, which was closely related to the regulation of gut microbial composition, and OTSSP167-mediated fecal microbiota transplantation effectively alleviated DSS-induced colitis.
34144192	1	85	theme	incurable	279:287	arg1	diseases					306:313	chronic recurrent and incurable gastrointestinal diseases	257:313	chronic recurrent and incurable gastrointestinal diseases with an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC)	257:421	INTRODUCTION Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), is a group of chronic recurrent and incurable gastrointestinal diseases with an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC).
34144192	7	86	theme	cancer	1973:1978	arg1	occurrence					1959:1968	the occurrence	1955:1968	the occurrence of cancer	1955:1978	Further exploration of the molecular mechanism revealed that OTSSP167 inhibited AKT/IKK/P65 and ERK/IKK/P65 signaling cascades both in vivo and in vitro, which may help alleviate intestinal inflammation and control the occurrence of cancer.
34144192	8	87	theme	more	2238:2241	arg1	options					2243:2249	more options	2238:2249	more options for clinical treatment	2238:2272	CONCLUSION Our findings lay a theoretical foundation for the use of OTSSP167 as a treatment for IBD and its inhibition of the occurrence of colitis-associated carcinogenesis; additionally, MELK may be a potentially effective target molecule, thus providing more options for clinical treatment.
34144192	5	88	dep	RESULTS	1097:1103	arg1	found					1108:1112	found	1108:1112	found that pharmacological inhibition of MELK was indicated to significantly alleviate the inflammatory response in mice with colitis, reduce intestinal damage, and effectively inhibit the occurrence and progression of colitis-propelled carcinogenesis, which was closely related to the regulation of gut microbial composition, and OTSSP167-mediated fecal microbiota transplantation effectively alleviated DSS-induced colitis	1108:1531	RESULTS We found that pharmacological inhibition of MELK was indicated to significantly alleviate the inflammatory response in mice with colitis, reduce intestinal damage, and effectively inhibit the occurrence and progression of colitis-propelled carcinogenesis, which was closely related to the regulation of gut microbial composition, and OTSSP167-mediated fecal microbiota transplantation effectively alleviated DSS-induced colitis.
34144192	5	89	theme	composition	1422:1432	arg1	regulation					1394:1403	the regulation	1390:1403	the regulation of gut microbial composition	1390:1432	RESULTS We found that pharmacological inhibition of MELK was indicated to significantly alleviate the inflammatory response in mice with colitis, reduce intestinal damage, and effectively inhibit the occurrence and progression of colitis-propelled carcinogenesis, which was closely related to the regulation of gut microbial composition, and OTSSP167-mediated fecal microbiota transplantation effectively alleviated DSS-induced colitis.
34144192	1	90	theme	diseases	306:313	arg1	group					248:252	a group	246:252	a group of chronic recurrent and incurable gastrointestinal diseases with an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC)	246:421	INTRODUCTION Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), is a group of chronic recurrent and incurable gastrointestinal diseases with an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC).
34144192	1	90	theme	diseases	306:313	arg1	INTRODUCTION					136:147	INTRODUCTION Inflammatory bowel disease (IBD)	136:180	INTRODUCTION Inflammatory bowel disease (IBD)	136:180	INTRODUCTION Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), is a group of chronic recurrent and incurable gastrointestinal diseases with an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC).
34144192	1	90	theme	diseases	306:313	arg1	diseases					306:313	chronic recurrent and incurable gastrointestinal diseases	257:313	chronic recurrent and incurable gastrointestinal diseases with an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC)	257:421	INTRODUCTION Inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn's disease (CD), is a group of chronic recurrent and incurable gastrointestinal diseases with an unknown etiology that leads to a high risk of developing colitis-associated colorectal cancer (CRC).
34144192	6	91	theme	OTSSP167	1547:1554	arg1	treatment					1556:1564	OTSSP167 treatment	1547:1564	OTSSP167 treatment	1547:1564	In addition, OTSSP167 treatment obviously inhibited ferroptosis in the intestinal tissue and suppressed macrophage infiltration and M1 polarization, which reduced the secretion of pro-inflammatory factors.
32600338	15	0	dep	CONCLUSIONS	1834:1844	arg1	help					1880:1883	help	1880:1883	could help develop innovative nonsteroidal anti-inflammatory drugs with minimal side effects and strong efficacy	1874:1985	CONCLUSIONS The discoveries made herein could help develop innovative nonsteroidal anti-inflammatory drugs with minimal side effects and strong efficacy.
32600338	7	1	theme	IL-6	782:785	arg1	production					787:796	IL-6 production	782:796	IL-6 production	782:796	TNF-α and IL-6 production were measured by enzyme-linked immunosorbent assay (ELISA).
32600338	12	2	theme	A-H	1450:1452	arg1	fractions					1440:1448	fractions	1440:1448	fractions A-H	1440:1452	The essential oils were fractionated into fractions A-H, and fraction F has been demonstrated to inhibit the expression levels of TNF-α, IL-6, NO, iNOS and COX-2 in LPS-activated RAW264.7 macrophages.
32600338	13	3	theme	fraction	1634:1641	arg1	F					1643:1643	fraction F	1634:1643	fraction F	1634:1643	Mechanistic analysis revealed that fraction F reduced the phosphorylation levels of ERK1/2, p38, PKC-α, PKC-ε and PKC-δ, and inhibited the NF-κB transcriptional activity.
32600338	12	4	theme	fraction	1459:1466	arg1	F					1468:1468	fraction F	1459:1468	fraction F	1459:1468	The essential oils were fractionated into fractions A-H, and fraction F has been demonstrated to inhibit the expression levels of TNF-α, IL-6, NO, iNOS and COX-2 in LPS-activated RAW264.7 macrophages.
32600338	5	5	theme	Alamar	691:696	arg1	assay					703:707	an Alamar Blue assay	688:707	an Alamar Blue assay	688:707	The cell survival was quantified by an Alamar Blue assay.
32600338	2	6	theme	antimicrobial	307:319	arg1	effects					321:327	antioxidant and antimicrobial effects	291:327	antioxidant and antimicrobial effects	291:327	It was reported that Eucalyptus leaves possess antioxidant and antimicrobial effects.
32600338	11	7	theme	RAW264.7	1376:1383	arg1	macrophages					1385:1395	LPS-activated RAW264.7 macrophages	1362:1395	LPS-activated RAW264.7 macrophages	1362:1395	RESULTS The essential oils extracted from Eucalyptus citriodora leaf exert the best NO inhibitory activity in LPS-activated RAW264.7 macrophages.
32600338	15	8	theme	strong	1971:1976	arg1	efficacy					1978:1985	strong efficacy	1971:1985	strong efficacy	1971:1985	CONCLUSIONS The discoveries made herein could help develop innovative nonsteroidal anti-inflammatory drugs with minimal side effects and strong efficacy.
32600338	13	9	theme	Mechanistic	1599:1609	arg1	analysis					1611:1618	Mechanistic analysis	1599:1618	Mechanistic analysis	1599:1618	Mechanistic analysis revealed that fraction F reduced the phosphorylation levels of ERK1/2, p38, PKC-α, PKC-ε and PKC-δ, and inhibited the NF-κB transcriptional activity.
32600338	16	10	theme	essential	2029:2037	arg1	oils					2039:2042	these Eucalyptus leaf essential oils	2007:2042	these Eucalyptus leaf essential oils	2007:2042	Clinical trials on these Eucalyptus leaf essential oils will help customize and optimize their therapeutic administration.
32600338	15	11	theme	minimal	1946:1952	arg1	effects					1959:1965	minimal side effects	1946:1965	minimal side effects	1946:1965	CONCLUSIONS The discoveries made herein could help develop innovative nonsteroidal anti-inflammatory drugs with minimal side effects and strong efficacy.
32600338	4	12	theme	Lipopolysaccharide	501:518	arg1	macrophages					545:555	Lipopolysaccharide (LPS)-activated RAW264.7 macrophages	501:555	Lipopolysaccharide (LPS)-activated RAW264.7 macrophages	501:555	METHODS Lipopolysaccharide (LPS)-activated RAW264.7 macrophages were used to evaluate the anti-inflammatory activity of the leaf essential oils of Eucalyptus.
32600338	13	13	theme	NF-κB	1738:1742	arg1	activity					1760:1767	the NF-κB transcriptional activity	1734:1767	the NF-κB transcriptional activity	1734:1767	Mechanistic analysis revealed that fraction F reduced the phosphorylation levels of ERK1/2, p38, PKC-α, PKC-ε and PKC-δ, and inhibited the NF-κB transcriptional activity.
32600338	12	14	theme	essential	1402:1410	arg1	oils					1412:1415	The essential oils	1398:1415	The essential oils	1398:1415	The essential oils were fractionated into fractions A-H, and fraction F has been demonstrated to inhibit the expression levels of TNF-α, IL-6, NO, iNOS and COX-2 in LPS-activated RAW264.7 macrophages.
32600338	15	15	theme	nonsteroidal	1904:1915	arg1	drugs					1935:1939	innovative nonsteroidal anti-inflammatory drugs	1893:1939	innovative nonsteroidal anti-inflammatory drugs with minimal side effects and strong efficacy	1893:1985	CONCLUSIONS The discoveries made herein could help develop innovative nonsteroidal anti-inflammatory drugs with minimal side effects and strong efficacy.
32600338	10	16	theme	mitogen-activated	1101:1117	arg1	MAPK					1135:1138	MAPK	1135:1138	MAPK	1135:1138	The expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and NF-κB pathway were measured by western blot in LPS-activated RAW 264.7 macrophage.
32600338	10	16	theme	mitogen-activated	1101:1117	arg1	kinase					1127:1132	mitogen-activated protein kinase	1101:1132	mitogen-activated protein kinase (MAPK)	1101:1139	The expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and NF-κB pathway were measured by western blot in LPS-activated RAW 264.7 macrophage.
32600338	14	17	theme	Fraction	1798:1805	arg1	F					1807:1807	Fraction F	1798:1807	Fraction F	1798:1807	The chemical composition of Fraction F was determined by GC-MS.
32600338	6	18	theme	oxide	717:721	arg1	production					728:737	Nitric oxide (NO) production	710:737	Nitric oxide (NO) production	710:737	Nitric oxide (NO) production was assessed by Griess reaction.
32600338	2	19	theme	antioxidant	291:301	arg1	effects					321:327	antioxidant and antimicrobial effects	291:327	antioxidant and antimicrobial effects	291:327	It was reported that Eucalyptus leaves possess antioxidant and antimicrobial effects.
32600338	1	20	theme	Eucalyptus	164:173	arg1	oils					185:188	BACKGROUND Eucalyptus essential oils	153:188	BACKGROUND Eucalyptus essential oils	153:188	BACKGROUND Eucalyptus essential oils have been used in traditional medicine for centuries.
32600338	12	21	theme	RAW264.7	1577:1584	arg1	macrophages					1586:1596	LPS-activated RAW264.7 macrophages	1563:1596	LPS-activated RAW264.7 macrophages	1563:1596	The essential oils were fractionated into fractions A-H, and fraction F has been demonstrated to inhibit the expression levels of TNF-α, IL-6, NO, iNOS and COX-2 in LPS-activated RAW264.7 macrophages.
32600338	3	22	theme	anti-inflammatory	356:372	arg1	activity					374:381	the anti-inflammatory activity	352:381	the anti-inflammatory activity of the essential oils extracted from the leaves of four different Eucalyptus species in RAW264.7 macrophages	352:490	Here, we investigated the anti-inflammatory activity of the essential oils extracted from the leaves of four different Eucalyptus species in RAW264.7 macrophages.
32600338	3	23	dep	the	420:422	arg1	leaves					424:429	leaves	424:429	leaves	424:429	Here, we investigated the anti-inflammatory activity of the essential oils extracted from the leaves of four different Eucalyptus species in RAW264.7 macrophages.
32600338	3	24	from	species	460:466	arg1	macrophages					480:490	RAW264.7 macrophages	471:490	RAW264.7 macrophages	471:490	Here, we investigated the anti-inflammatory activity of the essential oils extracted from the leaves of four different Eucalyptus species in RAW264.7 macrophages.
32600338	11	25	theme	Eucalyptus	1294:1303	arg1	leaf					1316:1319	Eucalyptus citriodora leaf	1294:1319	Eucalyptus citriodora leaf	1294:1319	RESULTS The essential oils extracted from Eucalyptus citriodora leaf exert the best NO inhibitory activity in LPS-activated RAW264.7 macrophages.
32600338	8	26	theme	Nuclear	858:864	arg1	activity					900:907	Nuclear factor-κB (NF-κB) transcriptional activity	858:907	Nuclear factor-κB (NF-κB) transcriptional activity	858:907	Nuclear factor-κB (NF-κB) transcriptional activity was measured by NF-κB reporter assay.
32600338	10	27	theme	kinase	1150:1155	arg1	PKC					1160:1162	PKC	1160:1162	PKC	1160:1162	The expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and NF-κB pathway were measured by western blot in LPS-activated RAW 264.7 macrophage.
32600338	10	27	theme	kinase	1150:1155	arg1	C					1157:1157	protein kinase C	1142:1157	protein kinase C (PKC)	1142:1163	The expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and NF-κB pathway were measured by western blot in LPS-activated RAW 264.7 macrophage.
32600338	16	28	theme	Eucalyptus	2013:2022	arg1	oils					2039:2042	these Eucalyptus leaf essential oils	2007:2042	these Eucalyptus leaf essential oils	2007:2042	Clinical trials on these Eucalyptus leaf essential oils will help customize and optimize their therapeutic administration.
32600338	8	29	theme	NF-κB	925:929	arg1	assay					940:944	NF-κB reporter assay	925:944	NF-κB reporter assay	925:944	Nuclear factor-κB (NF-κB) transcriptional activity was measured by NF-κB reporter assay.
32600338	3	30	theme	essential	390:398	arg1	oils					400:403	the essential oils	386:403	the essential oils extracted from the leaves of four different Eucalyptus species in RAW264.7 macrophages	386:490	Here, we investigated the anti-inflammatory activity of the essential oils extracted from the leaves of four different Eucalyptus species in RAW264.7 macrophages.
32600338	4	31	theme	-activated	525:534	arg1	macrophages					545:555	Lipopolysaccharide (LPS)-activated RAW264.7 macrophages	501:555	Lipopolysaccharide (LPS)-activated RAW264.7 macrophages	501:555	METHODS Lipopolysaccharide (LPS)-activated RAW264.7 macrophages were used to evaluate the anti-inflammatory activity of the leaf essential oils of Eucalyptus.
32600338	11	32	theme	NO	1336:1337	arg1	activity					1350:1357	the best NO inhibitory activity	1327:1357	the best NO inhibitory activity in LPS-activated RAW264.7 macrophages	1327:1395	RESULTS The essential oils extracted from Eucalyptus citriodora leaf exert the best NO inhibitory activity in LPS-activated RAW264.7 macrophages.
32600338	6	33	theme	Griess	755:760	arg1	reaction					762:769	Griess reaction	755:769	Griess reaction	755:769	Nitric oxide (NO) production was assessed by Griess reaction.
32600338	15	34	with	drugs	1935:1939	arg1	efficacy					1978:1985	strong efficacy	1971:1985	strong efficacy	1971:1985	CONCLUSIONS The discoveries made herein could help develop innovative nonsteroidal anti-inflammatory drugs with minimal side effects and strong efficacy.
32600338	15	34	with	drugs	1935:1939	arg1	effects					1959:1965	minimal side effects	1946:1965	minimal side effects	1946:1965	CONCLUSIONS The discoveries made herein could help develop innovative nonsteroidal anti-inflammatory drugs with minimal side effects and strong efficacy.
32600338	13	35	theme	PKC-ε	1703:1707	arg1	levels					1673:1678	the phosphorylation levels	1653:1678	the phosphorylation levels of ERK1/2, p38, PKC-α, PKC-ε and PKC-δ	1653:1717	Mechanistic analysis revealed that fraction F reduced the phosphorylation levels of ERK1/2, p38, PKC-α, PKC-ε and PKC-δ, and inhibited the NF-κB transcriptional activity.
32600338	3	36	theme	RAW264.7	471:478	arg1	macrophages					480:490	RAW264.7 macrophages	471:490	RAW264.7 macrophages	471:490	Here, we investigated the anti-inflammatory activity of the essential oils extracted from the leaves of four different Eucalyptus species in RAW264.7 macrophages.
32600338	0	37	theme	Eucalyptus	0:9	arg1	oils					21:24	Eucalyptus essential oils	0:24	Eucalyptus essential oils	0:24	Eucalyptus essential oils inhibit the lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages through reducing MAPK and NF-κB pathways.
32600338	4	38	theme	leaf	617:620	arg1	oils					632:635	the leaf essential oils	613:635	the leaf essential oils of Eucalyptus	613:649	METHODS Lipopolysaccharide (LPS)-activated RAW264.7 macrophages were used to evaluate the anti-inflammatory activity of the leaf essential oils of Eucalyptus.
32600338	13	39	theme	ERK1/2	1683:1688	arg1	levels					1673:1678	the phosphorylation levels	1653:1678	the phosphorylation levels of ERK1/2, p38, PKC-α, PKC-ε and PKC-δ	1653:1717	Mechanistic analysis revealed that fraction F reduced the phosphorylation levels of ERK1/2, p38, PKC-α, PKC-ε and PKC-δ, and inhibited the NF-κB transcriptional activity.
32600338	4	40	theme	oils	632:635	arg1	activity					601:608	the anti-inflammatory activity	579:608	the anti-inflammatory activity of the leaf essential oils of Eucalyptus	579:649	METHODS Lipopolysaccharide (LPS)-activated RAW264.7 macrophages were used to evaluate the anti-inflammatory activity of the leaf essential oils of Eucalyptus.
32600338	13	41	theme	p38	1691:1693	arg1	levels					1673:1678	the phosphorylation levels	1653:1678	the phosphorylation levels of ERK1/2, p38, PKC-α, PKC-ε and PKC-δ	1653:1717	Mechanistic analysis revealed that fraction F reduced the phosphorylation levels of ERK1/2, p38, PKC-α, PKC-ε and PKC-δ, and inhibited the NF-κB transcriptional activity.
32600338	7	42	link	enzyme-linked	815:827	arg1	assay					843:847	enzyme-linked immunosorbent assay	815:847	enzyme-linked immunosorbent assay (ELISA)	815:855	TNF-α and IL-6 production were measured by enzyme-linked immunosorbent assay (ELISA).
32600338	7	42	link	enzyme-linked	815:827	arg1	ELISA					850:854	ELISA	850:854	ELISA	850:854	TNF-α and IL-6 production were measured by enzyme-linked immunosorbent assay (ELISA).
32600338	3	43	theme	different	439:447	arg1	species					460:466	four different Eucalyptus species	434:466	four different Eucalyptus species in RAW264.7 macrophages	434:490	Here, we investigated the anti-inflammatory activity of the essential oils extracted from the leaves of four different Eucalyptus species in RAW264.7 macrophages.
32600338	4	44	theme	anti-inflammatory	583:599	arg1	activity					601:608	the anti-inflammatory activity	579:608	the anti-inflammatory activity of the leaf essential oils of Eucalyptus	579:649	METHODS Lipopolysaccharide (LPS)-activated RAW264.7 macrophages were used to evaluate the anti-inflammatory activity of the leaf essential oils of Eucalyptus.
32600338	0	45	theme	inflammatory	65:76	arg1	response					78:85	the lipopolysaccharide-induced inflammatory response	34:85	the lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages	34:109	Eucalyptus essential oils inhibit the lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages through reducing MAPK and NF-κB pathways.
32600338	10	46	theme	NF-κB	1169:1173	arg1	pathway					1175:1181	NF-κB pathway	1169:1181	NF-κB pathway	1169:1181	The expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and NF-κB pathway were measured by western blot in LPS-activated RAW 264.7 macrophage.
32600338	10	47	theme	expression	1024:1033	arg1	levels					1035:1040	The expression levels	1020:1040	The expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and NF-κB pathway	1020:1181	The expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and NF-κB pathway were measured by western blot in LPS-activated RAW 264.7 macrophage.
32600338	11	48	theme	essential	1264:1272	arg1	oils					1274:1277	The essential oils	1260:1277	The essential oils extracted from Eucalyptus citriodora leaf	1260:1319	RESULTS The essential oils extracted from Eucalyptus citriodora leaf exert the best NO inhibitory activity in LPS-activated RAW264.7 macrophages.
32600338	4	49	dep	METHODS	493:499	arg1	used					562:565	used	562:565	were used to evaluate the anti-inflammatory activity of the leaf essential oils of Eucalyptus	557:649	METHODS Lipopolysaccharide (LPS)-activated RAW264.7 macrophages were used to evaluate the anti-inflammatory activity of the leaf essential oils of Eucalyptus.
32600338	12	50	theme	TNF-α	1528:1532	arg1	levels					1518:1523	the expression levels	1503:1523	the expression levels of TNF-α, IL-6, NO, iNOS and COX-2 in LPS-activated RAW264.7 macrophages	1503:1596	The essential oils were fractionated into fractions A-H, and fraction F has been demonstrated to inhibit the expression levels of TNF-α, IL-6, NO, iNOS and COX-2 in LPS-activated RAW264.7 macrophages.
32600338	16	51	theme	Clinical	1988:1995	arg1	trials					1997:2002	Clinical trials	1988:2002	Clinical trials on these Eucalyptus leaf essential oils	1988:2042	Clinical trials on these Eucalyptus leaf essential oils will help customize and optimize their therapeutic administration.
32600338	10	52	theme	NO	1055:1056	arg1	synthase					1058:1065	inducible NO synthase	1045:1065	inducible NO synthase (iNOS)	1045:1072	The expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and NF-κB pathway were measured by western blot in LPS-activated RAW 264.7 macrophage.
32600338	10	52	theme	NO	1055:1056	arg1	iNOS					1068:1071	iNOS	1068:1071	iNOS	1068:1071	The expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and NF-κB pathway were measured by western blot in LPS-activated RAW 264.7 macrophage.
32600338	15	53	theme	side	1954:1957	arg1	effects					1959:1965	minimal side effects	1946:1965	minimal side effects	1946:1965	CONCLUSIONS The discoveries made herein could help develop innovative nonsteroidal anti-inflammatory drugs with minimal side effects and strong efficacy.
32600338	14	54	theme	F	1807:1807	arg1	composition					1783:1793	The chemical composition	1770:1793	The chemical composition of Fraction F	1770:1807	The chemical composition of Fraction F was determined by GC-MS.
32600338	11	55	dep	RESULTS	1252:1258	arg1	exert					1321:1325	exert	1321:1325	exert the best NO inhibitory activity in LPS-activated RAW264.7 macrophages	1321:1395	RESULTS The essential oils extracted from Eucalyptus citriodora leaf exert the best NO inhibitory activity in LPS-activated RAW264.7 macrophages.
32600338	4	56	theme	Eucalyptus	640:649	arg1	oils					632:635	the leaf essential oils	613:635	the leaf essential oils of Eucalyptus	613:649	METHODS Lipopolysaccharide (LPS)-activated RAW264.7 macrophages were used to evaluate the anti-inflammatory activity of the leaf essential oils of Eucalyptus.
32600338	9	57	theme	Western	1006:1012	arg1	blot					1014:1017	Western blot	1006:1017	Western blot	1006:1017	Intracellular protein expression levels were determined by Western blot.
32600338	10	58	theme	LPS-activated	1216:1228	arg1	RAW					1230:1232	LPS-activated RAW 264.7	1216:1238	LPS-activated RAW 264.7 macrophage	1216:1249	The expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and NF-κB pathway were measured by western blot in LPS-activated RAW 264.7 macrophage.
32600338	8	59	theme	transcriptional	884:898	arg1	activity					900:907	Nuclear factor-κB (NF-κB) transcriptional activity	858:907	Nuclear factor-κB (NF-κB) transcriptional activity	858:907	Nuclear factor-κB (NF-κB) transcriptional activity was measured by NF-κB reporter assay.
32600338	7	60	theme	enzyme-linked	815:827	arg1	assay					843:847	enzyme-linked immunosorbent assay	815:847	enzyme-linked immunosorbent assay (ELISA)	815:855	TNF-α and IL-6 production were measured by enzyme-linked immunosorbent assay (ELISA).
32600338	7	60	theme	enzyme-linked	815:827	arg1	ELISA					850:854	ELISA	850:854	ELISA	850:854	TNF-α and IL-6 production were measured by enzyme-linked immunosorbent assay (ELISA).
32600338	11	61	theme	LPS-activated	1362:1374	arg1	macrophages					1385:1395	LPS-activated RAW264.7 macrophages	1362:1395	LPS-activated RAW264.7 macrophages	1362:1395	RESULTS The essential oils extracted from Eucalyptus citriodora leaf exert the best NO inhibitory activity in LPS-activated RAW264.7 macrophages.
32600338	5	62	theme	Blue	698:701	arg1	assay					703:707	an Alamar Blue assay	688:707	an Alamar Blue assay	688:707	The cell survival was quantified by an Alamar Blue assay.
32600338	13	63	theme	phosphorylation	1657:1671	arg1	levels					1673:1678	the phosphorylation levels	1653:1678	the phosphorylation levels of ERK1/2, p38, PKC-α, PKC-ε and PKC-δ	1653:1717	Mechanistic analysis revealed that fraction F reduced the phosphorylation levels of ERK1/2, p38, PKC-α, PKC-ε and PKC-δ, and inhibited the NF-κB transcriptional activity.
32600338	1	64	theme	traditional	208:218	arg1	medicine					220:227	traditional medicine	208:227	traditional medicine for centuries	208:241	BACKGROUND Eucalyptus essential oils have been used in traditional medicine for centuries.
32600338	10	65	theme	kinase	1127:1132	arg1	levels					1035:1040	The expression levels	1020:1040	The expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and NF-κB pathway	1020:1181	The expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and NF-κB pathway were measured by western blot in LPS-activated RAW 264.7 macrophage.
32600338	15	66	theme	anti-inflammatory	1917:1933	arg1	drugs					1935:1939	innovative nonsteroidal anti-inflammatory drugs	1893:1939	innovative nonsteroidal anti-inflammatory drugs with minimal side effects and strong efficacy	1893:1985	CONCLUSIONS The discoveries made herein could help develop innovative nonsteroidal anti-inflammatory drugs with minimal side effects and strong efficacy.
32600338	10	67	from	blot	1208:1211	arg1	RAW					1230:1232	LPS-activated RAW 264.7	1216:1238	LPS-activated RAW 264.7 macrophage	1216:1249	The expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and NF-κB pathway were measured by western blot in LPS-activated RAW 264.7 macrophage.
32600338	10	68	theme	cyclooxygenase-2	1075:1090	arg1	levels					1035:1040	The expression levels	1020:1040	The expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and NF-κB pathway	1020:1181	The expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and NF-κB pathway were measured by western blot in LPS-activated RAW 264.7 macrophage.
32600338	13	69	theme	transcriptional	1744:1758	arg1	activity					1760:1767	the NF-κB transcriptional activity	1734:1767	the NF-κB transcriptional activity	1734:1767	Mechanistic analysis revealed that fraction F reduced the phosphorylation levels of ERK1/2, p38, PKC-α, PKC-ε and PKC-δ, and inhibited the NF-κB transcriptional activity.
32600338	13	70	theme	PKC-δ	1713:1717	arg1	levels					1673:1678	the phosphorylation levels	1653:1678	the phosphorylation levels of ERK1/2, p38, PKC-α, PKC-ε and PKC-δ	1653:1717	Mechanistic analysis revealed that fraction F reduced the phosphorylation levels of ERK1/2, p38, PKC-α, PKC-ε and PKC-δ, and inhibited the NF-κB transcriptional activity.
32600338	9	71	theme	protein	961:967	arg1	levels					980:985	Intracellular protein expression levels	947:985	Intracellular protein expression levels	947:985	Intracellular protein expression levels were determined by Western blot.
32600338	1	72	theme	BACKGROUND	153:162	arg1	oils					185:188	BACKGROUND Eucalyptus essential oils	153:188	BACKGROUND Eucalyptus essential oils	153:188	BACKGROUND Eucalyptus essential oils have been used in traditional medicine for centuries.
32600338	8	73	theme	factor-κB	866:874	arg1	activity					900:907	Nuclear factor-κB (NF-κB) transcriptional activity	858:907	Nuclear factor-κB (NF-κB) transcriptional activity	858:907	Nuclear factor-κB (NF-κB) transcriptional activity was measured by NF-κB reporter assay.
32600338	12	74	theme	LPS-activated	1563:1575	arg1	macrophages					1586:1596	LPS-activated RAW264.7 macrophages	1563:1596	LPS-activated RAW264.7 macrophages	1563:1596	The essential oils were fractionated into fractions A-H, and fraction F has been demonstrated to inhibit the expression levels of TNF-α, IL-6, NO, iNOS and COX-2 in LPS-activated RAW264.7 macrophages.
32600338	6	75	theme	Nitric	710:715	arg1	NO					724:725	NO	724:725	NO	724:725	Nitric oxide (NO) production was assessed by Griess reaction.
32600338	6	75	theme	Nitric	710:715	arg1	oxide					717:721	Nitric oxide	710:721	Nitric oxide (NO) production	710:737	Nitric oxide (NO) production was assessed by Griess reaction.
32600338	0	76	theme	RAW264.7	90:97	arg1	macrophages					99:109	RAW264.7 macrophages	90:109	RAW264.7 macrophages	90:109	Eucalyptus essential oils inhibit the lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages through reducing MAPK and NF-κB pathways.
32600338	1	77	theme	essential	175:183	arg1	oils					185:188	BACKGROUND Eucalyptus essential oils	153:188	BACKGROUND Eucalyptus essential oils	153:188	BACKGROUND Eucalyptus essential oils have been used in traditional medicine for centuries.
32600338	1	78	used	used	200:203	arg2	oils					185:188	BACKGROUND Eucalyptus essential oils	153:188	BACKGROUND Eucalyptus essential oils	153:188	BACKGROUND Eucalyptus essential oils have been used in traditional medicine for centuries.
32600338	0	79	from	response	78:85	arg1	macrophages					99:109	RAW264.7 macrophages	90:109	RAW264.7 macrophages	90:109	Eucalyptus essential oils inhibit the lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages through reducing MAPK and NF-κB pathways.
32600338	10	80	theme	protein	1119:1125	arg1	MAPK					1135:1138	MAPK	1135:1138	MAPK	1135:1138	The expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and NF-κB pathway were measured by western blot in LPS-activated RAW 264.7 macrophage.
32600338	10	80	theme	protein	1119:1125	arg1	kinase					1127:1132	mitogen-activated protein kinase	1101:1132	mitogen-activated protein kinase (MAPK)	1101:1139	The expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and NF-κB pathway were measured by western blot in LPS-activated RAW 264.7 macrophage.
32600338	16	81	theme	leaf	2024:2027	arg1	oils					2039:2042	these Eucalyptus leaf essential oils	2007:2042	these Eucalyptus leaf essential oils	2007:2042	Clinical trials on these Eucalyptus leaf essential oils will help customize and optimize their therapeutic administration.
32600338	4	82	theme	RAW264.7	536:543	arg1	macrophages					545:555	Lipopolysaccharide (LPS)-activated RAW264.7 macrophages	501:555	Lipopolysaccharide (LPS)-activated RAW264.7 macrophages	501:555	METHODS Lipopolysaccharide (LPS)-activated RAW264.7 macrophages were used to evaluate the anti-inflammatory activity of the leaf essential oils of Eucalyptus.
32600338	11	83	theme	citriodora	1305:1314	arg1	leaf					1316:1319	Eucalyptus citriodora leaf	1294:1319	Eucalyptus citriodora leaf	1294:1319	RESULTS The essential oils extracted from Eucalyptus citriodora leaf exert the best NO inhibitory activity in LPS-activated RAW264.7 macrophages.
32600338	0	84	theme	NF-κB	137:141	arg1	pathways					143:150	NF-κB pathways	137:150	NF-κB pathways	137:150	Eucalyptus essential oils inhibit the lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages through reducing MAPK and NF-κB pathways.
32600338	10	85	theme	protein	1142:1148	arg1	PKC					1160:1162	PKC	1160:1162	PKC	1160:1162	The expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and NF-κB pathway were measured by western blot in LPS-activated RAW 264.7 macrophage.
32600338	10	85	theme	protein	1142:1148	arg1	C					1157:1157	protein kinase C	1142:1157	protein kinase C (PKC)	1142:1163	The expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and NF-κB pathway were measured by western blot in LPS-activated RAW 264.7 macrophage.
32600338	12	86	from	COX-2	1554:1558	arg1	macrophages					1586:1596	LPS-activated RAW264.7 macrophages	1563:1596	LPS-activated RAW264.7 macrophages	1563:1596	The essential oils were fractionated into fractions A-H, and fraction F has been demonstrated to inhibit the expression levels of TNF-α, IL-6, NO, iNOS and COX-2 in LPS-activated RAW264.7 macrophages.
32600338	3	87	theme	oils	400:403	arg1	activity					374:381	the anti-inflammatory activity	352:381	the anti-inflammatory activity of the essential oils extracted from the leaves of four different Eucalyptus species in RAW264.7 macrophages	352:490	Here, we investigated the anti-inflammatory activity of the essential oils extracted from the leaves of four different Eucalyptus species in RAW264.7 macrophages.
32600338	11	88	theme	inhibitory	1339:1348	arg1	activity					1350:1357	the best NO inhibitory activity	1327:1357	the best NO inhibitory activity in LPS-activated RAW264.7 macrophages	1327:1395	RESULTS The essential oils extracted from Eucalyptus citriodora leaf exert the best NO inhibitory activity in LPS-activated RAW264.7 macrophages.
32600338	14	89	theme	chemical	1774:1781	arg1	composition					1783:1793	The chemical composition	1770:1793	The chemical composition of Fraction F	1770:1807	The chemical composition of Fraction F was determined by GC-MS.
32600338	0	90	theme	essential	11:19	arg1	oils					21:24	Eucalyptus essential oils	0:24	Eucalyptus essential oils	0:24	Eucalyptus essential oils inhibit the lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages through reducing MAPK and NF-κB pathways.
32600338	12	91	from	iNOS	1545:1548	arg1	macrophages					1586:1596	LPS-activated RAW264.7 macrophages	1563:1596	LPS-activated RAW264.7 macrophages	1563:1596	The essential oils were fractionated into fractions A-H, and fraction F has been demonstrated to inhibit the expression levels of TNF-α, IL-6, NO, iNOS and COX-2 in LPS-activated RAW264.7 macrophages.
32600338	8	92	theme	reporter	931:938	arg1	assay					940:944	NF-κB reporter assay	925:944	NF-κB reporter assay	925:944	Nuclear factor-κB (NF-κB) transcriptional activity was measured by NF-κB reporter assay.
32600338	4	93	theme	essential	622:630	arg1	oils					632:635	the leaf essential oils	613:635	the leaf essential oils of Eucalyptus	613:649	METHODS Lipopolysaccharide (LPS)-activated RAW264.7 macrophages were used to evaluate the anti-inflammatory activity of the leaf essential oils of Eucalyptus.
32600338	11	94	theme	best	1331:1334	arg1	activity					1350:1357	the best NO inhibitory activity	1327:1357	the best NO inhibitory activity in LPS-activated RAW264.7 macrophages	1327:1395	RESULTS The essential oils extracted from Eucalyptus citriodora leaf exert the best NO inhibitory activity in LPS-activated RAW264.7 macrophages.
32600338	13	95	theme	PKC-α	1696:1700	arg1	levels					1673:1678	the phosphorylation levels	1653:1678	the phosphorylation levels of ERK1/2, p38, PKC-α, PKC-ε and PKC-δ	1653:1717	Mechanistic analysis revealed that fraction F reduced the phosphorylation levels of ERK1/2, p38, PKC-α, PKC-ε and PKC-δ, and inhibited the NF-κB transcriptional activity.
32600338	4	96	used	used	562:565	arg2	macrophages					545:555	Lipopolysaccharide (LPS)-activated RAW264.7 macrophages	501:555	Lipopolysaccharide (LPS)-activated RAW264.7 macrophages	501:555	METHODS Lipopolysaccharide (LPS)-activated RAW264.7 macrophages were used to evaluate the anti-inflammatory activity of the leaf essential oils of Eucalyptus.
32600338	5	97	theme	cell	656:659	arg1	survival					661:668	The cell survival	652:668	The cell survival	652:668	The cell survival was quantified by an Alamar Blue assay.
32600338	16	98	from	trials	1997:2002	arg1	oils					2039:2042	these Eucalyptus leaf essential oils	2007:2042	these Eucalyptus leaf essential oils	2007:2042	Clinical trials on these Eucalyptus leaf essential oils will help customize and optimize their therapeutic administration.
32600338	0	99	theme	lipopolysaccharide-induced	38:63	arg1	response					78:85	the lipopolysaccharide-induced inflammatory response	34:85	the lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages	34:109	Eucalyptus essential oils inhibit the lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages through reducing MAPK and NF-κB pathways.
32600338	10	100	theme	C	1157:1157	arg1	levels					1035:1040	The expression levels	1020:1040	The expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and NF-κB pathway	1020:1181	The expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and NF-κB pathway were measured by western blot in LPS-activated RAW 264.7 macrophage.
32600338	10	101	theme	inducible	1045:1053	arg1	synthase					1058:1065	inducible NO synthase	1045:1065	inducible NO synthase (iNOS)	1045:1072	The expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and NF-κB pathway were measured by western blot in LPS-activated RAW 264.7 macrophage.
32600338	10	101	theme	inducible	1045:1053	arg1	iNOS					1068:1071	iNOS	1068:1071	iNOS	1068:1071	The expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and NF-κB pathway were measured by western blot in LPS-activated RAW 264.7 macrophage.
32600338	16	102	theme	therapeutic	2083:2093	arg1	administration					2095:2108	their therapeutic administration	2077:2108	their therapeutic administration	2077:2108	Clinical trials on these Eucalyptus leaf essential oils will help customize and optimize their therapeutic administration.
32600338	10	103	theme	pathway	1175:1181	arg1	levels					1035:1040	The expression levels	1020:1040	The expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and NF-κB pathway	1020:1181	The expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and NF-κB pathway were measured by western blot in LPS-activated RAW 264.7 macrophage.
32600338	10	104	dep	RAW	1230:1232	arg1	macrophage					1240:1249	macrophage	1240:1249	LPS-activated RAW 264.7 macrophage	1216:1249	The expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and NF-κB pathway were measured by western blot in LPS-activated RAW 264.7 macrophage.
32600338	3	105	theme	Eucalyptus	449:458	arg1	species					460:466	four different Eucalyptus species	434:466	four different Eucalyptus species in RAW264.7 macrophages	434:490	Here, we investigated the anti-inflammatory activity of the essential oils extracted from the leaves of four different Eucalyptus species in RAW264.7 macrophages.
32600338	9	106	theme	Intracellular	947:959	arg1	levels					980:985	Intracellular protein expression levels	947:985	Intracellular protein expression levels	947:985	Intracellular protein expression levels were determined by Western blot.
32600338	7	107	theme	immunosorbent	829:841	arg1	assay					843:847	enzyme-linked immunosorbent assay	815:847	enzyme-linked immunosorbent assay (ELISA)	815:855	TNF-α and IL-6 production were measured by enzyme-linked immunosorbent assay (ELISA).
32600338	7	107	theme	immunosorbent	829:841	arg1	ELISA					850:854	ELISA	850:854	ELISA	850:854	TNF-α and IL-6 production were measured by enzyme-linked immunosorbent assay (ELISA).
32600338	12	108	theme	expression	1507:1516	arg1	levels					1518:1523	the expression levels	1503:1523	the expression levels of TNF-α, IL-6, NO, iNOS and COX-2 in LPS-activated RAW264.7 macrophages	1503:1596	The essential oils were fractionated into fractions A-H, and fraction F has been demonstrated to inhibit the expression levels of TNF-α, IL-6, NO, iNOS and COX-2 in LPS-activated RAW264.7 macrophages.
32600338	9	109	theme	expression	969:978	arg1	levels					980:985	Intracellular protein expression levels	947:985	Intracellular protein expression levels	947:985	Intracellular protein expression levels were determined by Western blot.
32600338	11	110	from	activity	1350:1357	arg1	macrophages					1385:1395	LPS-activated RAW264.7 macrophages	1362:1395	LPS-activated RAW264.7 macrophages	1362:1395	RESULTS The essential oils extracted from Eucalyptus citriodora leaf exert the best NO inhibitory activity in LPS-activated RAW264.7 macrophages.
32600338	10	111	theme	western	1200:1206	arg1	blot					1208:1211	western blot	1200:1211	western blot in LPS-activated RAW 264.7 macrophage	1200:1249	The expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and NF-κB pathway were measured by western blot in LPS-activated RAW 264.7 macrophage.
32600338	10	112	theme	synthase	1058:1065	arg1	levels					1035:1040	The expression levels	1020:1040	The expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and NF-κB pathway	1020:1181	The expression levels of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK), protein kinase C (PKC) and NF-κB pathway were measured by western blot in LPS-activated RAW 264.7 macrophage.
32600338	15	113	theme	innovative	1893:1902	arg1	drugs					1935:1939	innovative nonsteroidal anti-inflammatory drugs	1893:1939	innovative nonsteroidal anti-inflammatory drugs with minimal side effects and strong efficacy	1893:1985	CONCLUSIONS The discoveries made herein could help develop innovative nonsteroidal anti-inflammatory drugs with minimal side effects and strong efficacy.
34420705	4	0	theme	%	410:410	arg1	citrate					419:425	1.5% sodium citrate	407:425	1.5% sodium citrate	407:425	The highest pectin extraction (≥7.2%) was carried out at 80 °C, 120 min with 1.5% sodium citrate in peel of both AS and NAS persimmon samples.
34420705	7	1	theme	glucose	904:910	arg1	levels					894:899	the highest levels	882:899	the highest levels of glucose and mannose, non-pectic carbohydrates	882:948	Remarkably, both pulp tissues (AS and NAS) presented the highest levels of glucose and mannose, non-pectic carbohydrates.
34420705	4	2	with	120 min	394:400	arg1	citrate					419:425	1.5% sodium citrate	407:425	1.5% sodium citrate	407:425	The highest pectin extraction (≥7.2%) was carried out at 80 °C, 120 min with 1.5% sodium citrate in peel of both AS and NAS persimmon samples.
34420705	6	3	theme	whole	808:812	arg1	samples					820:826	NAS persimmon whole fruit samples	794:826	NAS persimmon whole fruit samples	794:826	Pectin extracted from AS pulp and peel tissues exhibited an enriched structure in rhamnose and arabinose, whereas the opposite behavior was observed in NAS persimmon whole fruit samples.
34420705	2	4	theme	Rojo	115:118	arg1	brillante					120:128	Rojo brillante	115:128	Rojo brillante	115:128	Rojo brillante).
34420705	3	5	theme	astringent	226:235	arg1	fruits					277:282	astringent (AS) and non-astringent (NAS) persimmon fruits	226:282	astringent (AS) and non-astringent (NAS) persimmon fruits (peel, pulp, whole fruit)	226:308	In this work we have efficiently extracted and characterized pectin from different tissues of astringent (AS) and non-astringent (NAS) persimmon fruits (peel, pulp, whole fruit) for the first time.
34420705	4	6	theme	pectin	342:347	arg1	extraction					349:358	The highest pectin extraction	330:358	The highest pectin extraction (≥7.2%)	330:366	The highest pectin extraction (≥7.2%) was carried out at 80 °C, 120 min with 1.5% sodium citrate in peel of both AS and NAS persimmon samples.
34420705	4	6	theme	pectin	342:347	arg1	%					365:365	≥7.2%	361:365	≥7.2%	361:365	The highest pectin extraction (≥7.2%) was carried out at 80 °C, 120 min with 1.5% sodium citrate in peel of both AS and NAS persimmon samples.
34420705	8	7	theme	particle	1010:1017	arg1	size					1019:1022	particle size	1010:1022	particle size	1010:1022	In addition, techno-functional assessment (zeta potential, particle size, apparent viscosity, gelation) showed the suitability of the persimmon pectins for a broad range of industrial applications.
34420705	8	7	theme	particle	1010:1017	arg1	zeta					994:997	zeta potential	994:1007	zeta potential	994:1007	In addition, techno-functional assessment (zeta potential, particle size, apparent viscosity, gelation) showed the suitability of the persimmon pectins for a broad range of industrial applications.
34420705	6	8	theme	NAS	794:796	arg1	samples					820:826	NAS persimmon whole fruit samples	794:826	NAS persimmon whole fruit samples	794:826	Pectin extracted from AS pulp and peel tissues exhibited an enriched structure in rhamnose and arabinose, whereas the opposite behavior was observed in NAS persimmon whole fruit samples.
34420705	3	9	theme	different	205:213	arg1	tissues					215:221	different tissues	205:221	different tissues of astringent (AS) and non-astringent (NAS) persimmon fruits (peel, pulp, whole fruit)	205:308	In this work we have efficiently extracted and characterized pectin from different tissues of astringent (AS) and non-astringent (NAS) persimmon fruits (peel, pulp, whole fruit) for the first time.
34420705	4	10	theme	highest	334:340	arg1	extraction					349:358	The highest pectin extraction	330:358	The highest pectin extraction (≥7.2%)	330:366	The highest pectin extraction (≥7.2%) was carried out at 80 °C, 120 min with 1.5% sodium citrate in peel of both AS and NAS persimmon samples.
34420705	4	10	theme	highest	334:340	arg1	%					365:365	≥7.2%	361:365	≥7.2%	361:365	The highest pectin extraction (≥7.2%) was carried out at 80 °C, 120 min with 1.5% sodium citrate in peel of both AS and NAS persimmon samples.
34420705	3	11	dep	fruits	277:282	arg1	pulp					291:294	pulp	291:294	pulp	291:294	In this work we have efficiently extracted and characterized pectin from different tissues of astringent (AS) and non-astringent (NAS) persimmon fruits (peel, pulp, whole fruit) for the first time.
34420705	3	11	dep	fruits	277:282	arg1	fruit					303:307	whole fruit	297:307	whole fruit	297:307	In this work we have efficiently extracted and characterized pectin from different tissues of astringent (AS) and non-astringent (NAS) persimmon fruits (peel, pulp, whole fruit) for the first time.
34420705	3	11	dep	fruits	277:282	arg1	peel					285:288	peel	285:288	peel	285:288	In this work we have efficiently extracted and characterized pectin from different tissues of astringent (AS) and non-astringent (NAS) persimmon fruits (peel, pulp, whole fruit) for the first time.
34420705	5	12	theme	molecular	504:512	arg1	weight					514:519	a molecular weight	502:519	a molecular weight	502:519	All persimmon pectins showed a molecular weight and galacturonic acid content upper than 328 kDa and 78%, respectively, indicating their suitability as food ingredient.
34420705	7	13	theme	pulp	846:849	arg1	tissues					851:857	both pulp tissues	841:857	both pulp tissues (AS and NAS)	841:870	Remarkably, both pulp tissues (AS and NAS) presented the highest levels of glucose and mannose, non-pectic carbohydrates.
34420705	0	14	dep	Thunb	103:107	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction optimization and structural characterization of pectin from persimmon fruit (Diospyros kaki Thunb.
34420705	0	14	dep	Thunb	103:107	arg1	characterization					39:54	structural characterization	28:54	structural characterization	28:54	Extraction optimization and structural characterization of pectin from persimmon fruit (Diospyros kaki Thunb.
34420705	6	15	located	observed	782:789	arg2	behavior					769:776	the opposite behavior	756:776	the opposite behavior	756:776	Pectin extracted from AS pulp and peel tissues exhibited an enriched structure in rhamnose and arabinose, whereas the opposite behavior was observed in NAS persimmon whole fruit samples.
34420705	6	15	located	observed	782:789	arg1	samples					820:826	NAS persimmon whole fruit samples	794:826	NAS persimmon whole fruit samples	794:826	Pectin extracted from AS pulp and peel tissues exhibited an enriched structure in rhamnose and arabinose, whereas the opposite behavior was observed in NAS persimmon whole fruit samples.
34420705	8	16	theme	persimmon	1085:1093	arg1	pectins					1095:1101	the persimmon pectins	1081:1101	the persimmon pectins	1081:1101	In addition, techno-functional assessment (zeta potential, particle size, apparent viscosity, gelation) showed the suitability of the persimmon pectins for a broad range of industrial applications.
34420705	4	17	theme	1.5	407:409	arg1	%					410:410	%	410:410	%	410:410	The highest pectin extraction (≥7.2%) was carried out at 80 °C, 120 min with 1.5% sodium citrate in peel of both AS and NAS persimmon samples.
34420705	5	18	theme	galacturonic	525:536	arg1	content					543:549	galacturonic acid content	525:549	galacturonic acid content	525:549	All persimmon pectins showed a molecular weight and galacturonic acid content upper than 328 kDa and 78%, respectively, indicating their suitability as food ingredient.
34420705	8	19	theme	techno-functional	964:980	arg1	assessment					982:991	techno-functional assessment	964:991	techno-functional assessment (zeta potential, particle size, apparent viscosity, gelation)	964:1053	In addition, techno-functional assessment (zeta potential, particle size, apparent viscosity, gelation) showed the suitability of the persimmon pectins for a broad range of industrial applications.
34420705	6	20	theme	fruit	814:818	arg1	samples					820:826	NAS persimmon whole fruit samples	794:826	NAS persimmon whole fruit samples	794:826	Pectin extracted from AS pulp and peel tissues exhibited an enriched structure in rhamnose and arabinose, whereas the opposite behavior was observed in NAS persimmon whole fruit samples.
34420705	5	21	theme	acid	538:541	arg1	content					543:549	galacturonic acid content	525:549	galacturonic acid content	525:549	All persimmon pectins showed a molecular weight and galacturonic acid content upper than 328 kDa and 78%, respectively, indicating their suitability as food ingredient.
34420705	6	22	theme	peel	676:679	arg1	tissues					681:687	peel tissues	676:687	peel tissues	676:687	Pectin extracted from AS pulp and peel tissues exhibited an enriched structure in rhamnose and arabinose, whereas the opposite behavior was observed in NAS persimmon whole fruit samples.
34420705	7	23	theme	mannose	916:922	arg1	levels					894:899	the highest levels	882:899	the highest levels of glucose and mannose, non-pectic carbohydrates	882:948	Remarkably, both pulp tissues (AS and NAS) presented the highest levels of glucose and mannose, non-pectic carbohydrates.
34420705	6	24	from	structure	711:719	arg1	arabinose					737:745	arabinose	737:745	arabinose	737:745	Pectin extracted from AS pulp and peel tissues exhibited an enriched structure in rhamnose and arabinose, whereas the opposite behavior was observed in NAS persimmon whole fruit samples.
34420705	6	24	from	structure	711:719	arg1	rhamnose					724:731	rhamnose	724:731	rhamnose	724:731	Pectin extracted from AS pulp and peel tissues exhibited an enriched structure in rhamnose and arabinose, whereas the opposite behavior was observed in NAS persimmon whole fruit samples.
34420705	6	25	theme	persimmon	798:806	arg1	samples					820:826	NAS persimmon whole fruit samples	794:826	NAS persimmon whole fruit samples	794:826	Pectin extracted from AS pulp and peel tissues exhibited an enriched structure in rhamnose and arabinose, whereas the opposite behavior was observed in NAS persimmon whole fruit samples.
34420705	4	26	theme	NAS	450:452	arg1	peel					430:433	peel	430:433	peel of both AS and NAS persimmon samples	430:470	The highest pectin extraction (≥7.2%) was carried out at 80 °C, 120 min with 1.5% sodium citrate in peel of both AS and NAS persimmon samples.
34420705	5	27	theme	persimmon	477:485	arg1	pectins					487:493	All persimmon pectins	473:493	All persimmon pectins	473:493	All persimmon pectins showed a molecular weight and galacturonic acid content upper than 328 kDa and 78%, respectively, indicating their suitability as food ingredient.
34420705	8	28	theme	broad	1109:1113	arg1	range					1115:1119	a broad range	1107:1119	a broad range of industrial applications	1107:1146	In addition, techno-functional assessment (zeta potential, particle size, apparent viscosity, gelation) showed the suitability of the persimmon pectins for a broad range of industrial applications.
34420705	0	29	theme	structural	28:37	arg1	characterization					39:54	structural characterization	28:54	structural characterization	28:54	Extraction optimization and structural characterization of pectin from persimmon fruit (Diospyros kaki Thunb.
34420705	8	30	dep	assessment	982:991	arg1	size					1019:1022	particle size	1010:1022	particle size	1010:1022	In addition, techno-functional assessment (zeta potential, particle size, apparent viscosity, gelation) showed the suitability of the persimmon pectins for a broad range of industrial applications.
34420705	8	30	dep	assessment	982:991	arg1	viscosity					1034:1042	apparent viscosity	1025:1042	apparent viscosity	1025:1042	In addition, techno-functional assessment (zeta potential, particle size, apparent viscosity, gelation) showed the suitability of the persimmon pectins for a broad range of industrial applications.
34420705	8	30	dep	assessment	982:991	arg1	zeta					994:997	zeta potential	994:1007	zeta potential	994:1007	In addition, techno-functional assessment (zeta potential, particle size, apparent viscosity, gelation) showed the suitability of the persimmon pectins for a broad range of industrial applications.
34420705	8	30	dep	assessment	982:991	arg1	gelation					1045:1052	gelation	1045:1052	gelation	1045:1052	In addition, techno-functional assessment (zeta potential, particle size, apparent viscosity, gelation) showed the suitability of the persimmon pectins for a broad range of industrial applications.
34420705	8	31	theme	industrial	1124:1133	arg1	applications					1135:1146	industrial applications	1124:1146	industrial applications	1124:1146	In addition, techno-functional assessment (zeta potential, particle size, apparent viscosity, gelation) showed the suitability of the persimmon pectins for a broad range of industrial applications.
34420705	3	32	dep	astringent	226:235	arg1	AS					238:239	AS	238:239	AS	238:239	In this work we have efficiently extracted and characterized pectin from different tissues of astringent (AS) and non-astringent (NAS) persimmon fruits (peel, pulp, whole fruit) for the first time.
34420705	6	33	theme	opposite	760:767	arg1	behavior					769:776	the opposite behavior	756:776	the opposite behavior	756:776	Pectin extracted from AS pulp and peel tissues exhibited an enriched structure in rhamnose and arabinose, whereas the opposite behavior was observed in NAS persimmon whole fruit samples.
34420705	4	34	theme	persimmon	454:462	arg1	samples					464:470	persimmon samples	454:470	persimmon samples	454:470	The highest pectin extraction (≥7.2%) was carried out at 80 °C, 120 min with 1.5% sodium citrate in peel of both AS and NAS persimmon samples.
34420705	3	35	theme	non-astringent	246:259	arg1	fruits					277:282	astringent (AS) and non-astringent (NAS) persimmon fruits	226:282	astringent (AS) and non-astringent (NAS) persimmon fruits (peel, pulp, whole fruit)	226:308	In this work we have efficiently extracted and characterized pectin from different tissues of astringent (AS) and non-astringent (NAS) persimmon fruits (peel, pulp, whole fruit) for the first time.
34420705	7	36	theme	non-pectic	925:934	arg1	carbohydrates					936:948	non-pectic carbohydrates	925:948	non-pectic carbohydrates	925:948	Remarkably, both pulp tissues (AS and NAS) presented the highest levels of glucose and mannose, non-pectic carbohydrates.
34420705	7	36	theme	non-pectic	925:934	arg1	glucose					904:910	glucose	904:910	glucose	904:910	Remarkably, both pulp tissues (AS and NAS) presented the highest levels of glucose and mannose, non-pectic carbohydrates.
34420705	0	37	from	fruit	81:85	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction optimization and structural characterization of pectin from persimmon fruit (Diospyros kaki Thunb.
34420705	0	37	from	fruit	81:85	arg1	characterization					39:54	structural characterization	28:54	structural characterization	28:54	Extraction optimization and structural characterization of pectin from persimmon fruit (Diospyros kaki Thunb.
34420705	3	38	theme	first	318:322	arg1	time					324:327	the first time	314:327	the first time	314:327	In this work we have efficiently extracted and characterized pectin from different tissues of astringent (AS) and non-astringent (NAS) persimmon fruits (peel, pulp, whole fruit) for the first time.
34420705	6	39	theme	AS	664:665	arg1	pulp					667:670	AS pulp	664:670	AS pulp	664:670	Pectin extracted from AS pulp and peel tissues exhibited an enriched structure in rhamnose and arabinose, whereas the opposite behavior was observed in NAS persimmon whole fruit samples.
34420705	8	40	theme	applications	1135:1146	arg1	range					1115:1119	a broad range	1107:1119	a broad range of industrial applications	1107:1146	In addition, techno-functional assessment (zeta potential, particle size, apparent viscosity, gelation) showed the suitability of the persimmon pectins for a broad range of industrial applications.
34420705	8	41	theme	pectins	1095:1101	arg1	suitability					1066:1076	the suitability	1062:1076	the suitability of the persimmon pectins for a broad range of industrial applications	1062:1146	In addition, techno-functional assessment (zeta potential, particle size, apparent viscosity, gelation) showed the suitability of the persimmon pectins for a broad range of industrial applications.
34420705	3	42	from	tissues	215:221	arg1	pectin					193:198	pectin	193:198	pectin from different tissues of astringent (AS) and non-astringent (NAS) persimmon fruits (peel, pulp, whole fruit) for the first time	193:327	In this work we have efficiently extracted and characterized pectin from different tissues of astringent (AS) and non-astringent (NAS) persimmon fruits (peel, pulp, whole fruit) for the first time.
34420705	0	43	theme	persimmon	71:79	arg1	fruit					81:85	persimmon fruit	71:85	persimmon fruit	71:85	Extraction optimization and structural characterization of pectin from persimmon fruit (Diospyros kaki Thunb.
34420705	8	44	theme	potential	999:1007	arg1	size					1019:1022	particle size	1010:1022	particle size	1010:1022	In addition, techno-functional assessment (zeta potential, particle size, apparent viscosity, gelation) showed the suitability of the persimmon pectins for a broad range of industrial applications.
34420705	8	44	theme	potential	999:1007	arg1	viscosity					1034:1042	apparent viscosity	1025:1042	apparent viscosity	1025:1042	In addition, techno-functional assessment (zeta potential, particle size, apparent viscosity, gelation) showed the suitability of the persimmon pectins for a broad range of industrial applications.
34420705	8	44	theme	potential	999:1007	arg1	zeta					994:997	zeta potential	994:1007	zeta potential	994:1007	In addition, techno-functional assessment (zeta potential, particle size, apparent viscosity, gelation) showed the suitability of the persimmon pectins for a broad range of industrial applications.
34420705	8	44	theme	potential	999:1007	arg1	gelation					1045:1052	gelation	1045:1052	gelation	1045:1052	In addition, techno-functional assessment (zeta potential, particle size, apparent viscosity, gelation) showed the suitability of the persimmon pectins for a broad range of industrial applications.
34420705	0	45	theme	pectin	59:64	arg1	Extraction					0:9	Extraction	0:9	Extraction	0:9	Extraction optimization and structural characterization of pectin from persimmon fruit (Diospyros kaki Thunb.
34420705	0	45	theme	pectin	59:64	arg1	characterization					39:54	structural characterization	28:54	structural characterization	28:54	Extraction optimization and structural characterization of pectin from persimmon fruit (Diospyros kaki Thunb.
34420705	4	46	theme	AS	443:444	arg1	peel					430:433	peel	430:433	peel of both AS and NAS persimmon samples	430:470	The highest pectin extraction (≥7.2%) was carried out at 80 °C, 120 min with 1.5% sodium citrate in peel of both AS and NAS persimmon samples.
34420705	4	47	dep	AS	443:444	arg1	samples					464:470	persimmon samples	454:470	persimmon samples	454:470	The highest pectin extraction (≥7.2%) was carried out at 80 °C, 120 min with 1.5% sodium citrate in peel of both AS and NAS persimmon samples.
34420705	3	48	theme	persimmon	267:275	arg1	fruits					277:282	astringent (AS) and non-astringent (NAS) persimmon fruits	226:282	astringent (AS) and non-astringent (NAS) persimmon fruits (peel, pulp, whole fruit)	226:308	In this work we have efficiently extracted and characterized pectin from different tissues of astringent (AS) and non-astringent (NAS) persimmon fruits (peel, pulp, whole fruit) for the first time.
34420705	8	49	theme	apparent	1025:1032	arg1	viscosity					1034:1042	apparent viscosity	1025:1042	apparent viscosity	1025:1042	In addition, techno-functional assessment (zeta potential, particle size, apparent viscosity, gelation) showed the suitability of the persimmon pectins for a broad range of industrial applications.
34420705	8	49	theme	apparent	1025:1032	arg1	zeta					994:997	zeta potential	994:1007	zeta potential	994:1007	In addition, techno-functional assessment (zeta potential, particle size, apparent viscosity, gelation) showed the suitability of the persimmon pectins for a broad range of industrial applications.
34420705	6	50	theme	enriched	702:709	arg1	structure					711:719	an enriched structure	699:719	an enriched structure in rhamnose and arabinose	699:745	Pectin extracted from AS pulp and peel tissues exhibited an enriched structure in rhamnose and arabinose, whereas the opposite behavior was observed in NAS persimmon whole fruit samples.
34420705	5	51	dep	upper	551:555	arg1	than					557:560	than	557:560	than	557:560	All persimmon pectins showed a molecular weight and galacturonic acid content upper than 328 kDa and 78%, respectively, indicating their suitability as food ingredient.
34420705	3	52	theme	fruits	277:282	arg1	tissues					215:221	different tissues	205:221	different tissues of astringent (AS) and non-astringent (NAS) persimmon fruits (peel, pulp, whole fruit)	205:308	In this work we have efficiently extracted and characterized pectin from different tissues of astringent (AS) and non-astringent (NAS) persimmon fruits (peel, pulp, whole fruit) for the first time.
34420705	7	53	theme	highest	886:892	arg1	levels					894:899	the highest levels	882:899	the highest levels of glucose and mannose, non-pectic carbohydrates	882:948	Remarkably, both pulp tissues (AS and NAS) presented the highest levels of glucose and mannose, non-pectic carbohydrates.
34420705	7	54	dep	tissues	851:857	arg1	NAS					867:869	NAS	867:869	NAS	867:869	Remarkably, both pulp tissues (AS and NAS) presented the highest levels of glucose and mannose, non-pectic carbohydrates.
34420705	7	54	dep	tissues	851:857	arg1	AS					860:861	AS	860:861	AS	860:861	Remarkably, both pulp tissues (AS and NAS) presented the highest levels of glucose and mannose, non-pectic carbohydrates.
34420705	3	55	dep	non-astringent	246:259	arg1	NAS					262:264	NAS	262:264	NAS	262:264	In this work we have efficiently extracted and characterized pectin from different tissues of astringent (AS) and non-astringent (NAS) persimmon fruits (peel, pulp, whole fruit) for the first time.
34420705	3	56	theme	whole	297:301	arg1	fruit					303:307	whole fruit	297:307	whole fruit	297:307	In this work we have efficiently extracted and characterized pectin from different tissues of astringent (AS) and non-astringent (NAS) persimmon fruits (peel, pulp, whole fruit) for the first time.
34420705	3	56	theme	whole	297:301	arg1	peel					285:288	peel	285:288	peel	285:288	In this work we have efficiently extracted and characterized pectin from different tissues of astringent (AS) and non-astringent (NAS) persimmon fruits (peel, pulp, whole fruit) for the first time.
34420705	4	57	theme	sodium	412:417	arg1	citrate					419:425	1.5% sodium citrate	407:425	1.5% sodium citrate	407:425	The highest pectin extraction (≥7.2%) was carried out at 80 °C, 120 min with 1.5% sodium citrate in peel of both AS and NAS persimmon samples.
34420705	0	58	dep	Extraction	0:9	arg1	optimization					11:22	optimization	11:22	optimization	11:22	Extraction optimization and structural characterization of pectin from persimmon fruit (Diospyros kaki Thunb.
34420705	5	59	theme	food	625:628	arg1	ingredient					630:639	food ingredient	625:639	food ingredient	625:639	All persimmon pectins showed a molecular weight and galacturonic acid content upper than 328 kDa and 78%, respectively, indicating their suitability as food ingredient.
31530754	9	0	from	modulus	1484:1490	arg1	range					1530:1534	the physiological range	1512:1534	the physiological range of native myocardium (20∼100 kPa)	1512:1568	TPP crosslinked scaffolds showed excellent mechanical properties, with their elastic modulus (81.0 ± 8.1 kPa) in the physiological range of native myocardium (20∼100 kPa).
31530754	4	1	theme	scaffolds	635:643	arg1	properties					617:626	the biocompatibility and mechanical properties	581:626	the biocompatibility and mechanical properties of the scaffolds	581:643	Due to the non-proliferative and contractile characteristics of cardiomyocytes, the biocompatibility and mechanical properties of the scaffolds are extremely important for supporting intercellular connection and tissue function for myocardial tissue engineering.
31530754	4	1	theme	scaffolds	635:643	arg1	important					659:667	important	659:667	important	659:667	Due to the non-proliferative and contractile characteristics of cardiomyocytes, the biocompatibility and mechanical properties of the scaffolds are extremely important for supporting intercellular connection and tissue function for myocardial tissue engineering.
31530754	10	2	theme	TPP	1588:1590	arg1	scaffolds					1604:1612	GP and TPP crosslinked scaffolds	1581:1612	GP and TPP crosslinked scaffolds	1581:1612	Moreover, GP and TPP crosslinked scaffolds exhibited better biocompatibility than GTA crosslinked scaffolds, as demonstrated by the live/dead staining and proliferation assay.
31530754	10	3	theme	GP	1581:1582	arg1	scaffolds					1604:1612	GP and TPP crosslinked scaffolds	1581:1612	GP and TPP crosslinked scaffolds	1581:1612	Moreover, GP and TPP crosslinked scaffolds exhibited better biocompatibility than GTA crosslinked scaffolds, as demonstrated by the live/dead staining and proliferation assay.
31530754	0	4	theme	myocardial	77:86	arg1	engineering					95:105	myocardial tissue engineering	77:105	myocardial tissue engineering	77:105	Assessment of various crosslinking agents on collagen/chitosan scaffolds for myocardial tissue engineering.
31530754	4	5	theme	myocardial	733:742	arg1	engineering					751:761	myocardial tissue engineering	733:761	myocardial tissue engineering	733:761	Due to the non-proliferative and contractile characteristics of cardiomyocytes, the biocompatibility and mechanical properties of the scaffolds are extremely important for supporting intercellular connection and tissue function for myocardial tissue engineering.
31530754	10	6	theme	better	1624:1629	arg1	biocompatibility					1631:1646	better biocompatibility	1624:1646	better biocompatibility than GTA crosslinked scaffolds	1624:1677	Moreover, GP and TPP crosslinked scaffolds exhibited better biocompatibility than GTA crosslinked scaffolds, as demonstrated by the live/dead staining and proliferation assay.
31530754	11	7	theme	protein	1865:1871	arg1	expression					1827:1836	the highest expression	1815:1836	the highest expression of cardiac-specific marker protein	1815:1871	In addition, cardiomyocytes within TPP crosslinked scaffolds showed the highest expression of cardiac-specific marker protein and the best contractile performance.
31530754	11	7	theme	protein	1865:1871	arg1	performance					1898:1908	the best contractile performance	1877:1908	the best contractile performance	1877:1908	In addition, cardiomyocytes within TPP crosslinked scaffolds showed the highest expression of cardiac-specific marker protein and the best contractile performance.
31530754	12	8	theme	myocardial	2052:2061	arg1	engineering					2070:2080	myocardial tissue engineering	2052:2080	myocardial tissue engineering	2052:2080	To conclude, of the three crosslinking agents, TPP was recommended as the most suitable crosslinking agent for collagen/chitosan scaffold in myocardial tissue engineering.
31530754	4	9	theme	intercellular	684:696	arg1	connection					698:707	intercellular connection	684:707	intercellular connection	684:707	Due to the non-proliferative and contractile characteristics of cardiomyocytes, the biocompatibility and mechanical properties of the scaffolds are extremely important for supporting intercellular connection and tissue function for myocardial tissue engineering.
31530754	11	10	theme	cardiac-specific	1841:1856	arg1	protein					1865:1871	cardiac-specific marker protein	1841:1871	cardiac-specific marker protein	1841:1871	In addition, cardiomyocytes within TPP crosslinked scaffolds showed the highest expression of cardiac-specific marker protein and the best contractile performance.
31530754	2	11	theme	natural	320:326	arg1	polymers					328:335	natural polymers	320:335	natural polymers including collagen, gelatin, chitosan, fibrin, alginate, etc	320:396	Much research effort has been focused on natural polymers including collagen, gelatin, chitosan, fibrin, alginate, etc.
31530754	2	11	theme	natural	320:326	arg1	collagen					347:354	collagen	347:354	collagen	347:354	Much research effort has been focused on natural polymers including collagen, gelatin, chitosan, fibrin, alginate, etc.
31530754	12	12	theme	crosslinking	1999:2010	arg1	agent					2012:2016	the most suitable crosslinking agent	1981:2016	the most suitable crosslinking agent	1981:2016	To conclude, of the three crosslinking agents, TPP was recommended as the most suitable crosslinking agent for collagen/chitosan scaffold in myocardial tissue engineering.
31530754	10	13	theme	crosslinked	1657:1667	arg1	scaffolds					1669:1677	GTA crosslinked scaffolds	1653:1677	GTA crosslinked scaffolds	1653:1677	Moreover, GP and TPP crosslinked scaffolds exhibited better biocompatibility than GTA crosslinked scaffolds, as demonstrated by the live/dead staining and proliferation assay.
31530754	11	14	theme	contractile	1886:1896	arg1	performance					1898:1908	the best contractile performance	1877:1908	the best contractile performance	1877:1908	In addition, cardiomyocytes within TPP crosslinked scaffolds showed the highest expression of cardiac-specific marker protein and the best contractile performance.
31530754	5	15	theme	myocardial	928:937	arg1	engineering					946:956	myocardial tissue engineering	928:956	myocardial tissue engineering	928:956	To the best of our knowledge, the three crosslinking agents (glutaraldehyde (GTA), genipin (GP), tripolyphosphate (TPP)) have not yet been comparatively studied in myocardial tissue engineering.
31530754	2	16	theme	research	284:291	arg1	effort					293:298	Much research effort	279:298	Much research effort	279:298	Much research effort has been focused on natural polymers including collagen, gelatin, chitosan, fibrin, alginate, etc.
31530754	9	17	theme	native	1539:1544	arg1	myocardium					1546:1555	native myocardium	1539:1555	native myocardium (20∼100 kPa)	1539:1568	TPP crosslinked scaffolds showed excellent mechanical properties, with their elastic modulus (81.0 ± 8.1 kPa) in the physiological range of native myocardium (20∼100 kPa).
31530754	9	17	theme	native	1539:1544	arg1	kPa					1565:1567	20∼100 kPa	1558:1567	20∼100 kPa	1558:1567	TPP crosslinked scaffolds showed excellent mechanical properties, with their elastic modulus (81.0 ± 8.1 kPa) in the physiological range of native myocardium (20∼100 kPa).
31530754	6	18	theme	crosslinking	1018:1029	arg1	effect					1031:1036	the crosslinking effect	1014:1036	the crosslinking effect of GTA, GP and TPP for myocardial tissue engineering	1014:1089	Thus, the aim of this study is to compare and identify the crosslinking effect of GTA, GP and TPP for myocardial tissue engineering.
31530754	8	19	theme	good	1335:1338	arg1	ratio					1349:1353	good swelling ratio	1335:1353	good swelling ratio suitable for myocardial tissue engineering	1335:1396	All the groups of scaffolds exhibited high porosity (>65%) and good swelling ratio suitable for myocardial tissue engineering.
31530754	0	20	theme	tissue	88:93	arg1	engineering					95:105	myocardial tissue engineering	77:105	myocardial tissue engineering	77:105	Assessment of various crosslinking agents on collagen/chitosan scaffolds for myocardial tissue engineering.
31530754	0	21	from	Assessment	0:9	arg1	collagen/chitosan					45:61	collagen/chitosan	45:61	collagen/chitosan	45:61	Assessment of various crosslinking agents on collagen/chitosan scaffolds for myocardial tissue engineering.
31530754	8	22	theme	tissue	1379:1384	arg1	engineering					1386:1396	myocardial tissue engineering	1368:1396	myocardial tissue engineering	1368:1396	All the groups of scaffolds exhibited high porosity (>65%) and good swelling ratio suitable for myocardial tissue engineering.
31530754	11	23	link	crosslinked	1786:1796	arg1	scaffolds					1798:1806	TPP crosslinked scaffolds	1782:1806	TPP crosslinked scaffolds	1782:1806	In addition, cardiomyocytes within TPP crosslinked scaffolds showed the highest expression of cardiac-specific marker protein and the best contractile performance.
31530754	3	24	theme	composite	431:439	arg1	scaffold					441:448	a collagen/chitosan composite scaffold	411:448	a collagen/chitosan composite scaffold	411:448	Among them, a collagen/chitosan composite scaffold was widely used for myocardial tissue engineering.
31530754	1	25	theme	Suitable	108:115	arg1	material					117:124	Suitable material	108:124	Suitable material for scaffolds that support cell attachment, proliferation, vascularization and contraction	108:215	Suitable material for scaffolds that support cell attachment, proliferation, vascularization and contraction has always been a challenge in myocardial tissue engineering.
31530754	1	25	theme	Suitable	108:115	arg1	challenge					235:243	a challenge	233:243	a challenge in myocardial tissue engineering	233:276	Suitable material for scaffolds that support cell attachment, proliferation, vascularization and contraction has always been a challenge in myocardial tissue engineering.
31530754	3	26	theme	myocardial	470:479	arg1	engineering					488:498	myocardial tissue engineering	470:498	myocardial tissue engineering	470:498	Among them, a collagen/chitosan composite scaffold was widely used for myocardial tissue engineering.
31530754	0	27	theme	various	14:20	arg1	agents					35:40	various crosslinking agents	14:40	various crosslinking agents	14:40	Assessment of various crosslinking agents on collagen/chitosan scaffolds for myocardial tissue engineering.
31530754	10	28	theme	live/dead	1703:1711	arg1	staining					1713:1720	the live/dead staining	1699:1720	the live/dead staining	1699:1720	Moreover, GP and TPP crosslinked scaffolds exhibited better biocompatibility than GTA crosslinked scaffolds, as demonstrated by the live/dead staining and proliferation assay.
31530754	0	29	theme	agents	35:40	arg1	Assessment					0:9	Assessment	0:9	Assessment of various crosslinking agents on collagen/chitosan	0:61	Assessment of various crosslinking agents on collagen/chitosan scaffolds for myocardial tissue engineering.
31530754	12	30	from	scaffold	2040:2047	arg1	engineering					2070:2080	myocardial tissue engineering	2052:2080	myocardial tissue engineering	2052:2080	To conclude, of the three crosslinking agents, TPP was recommended as the most suitable crosslinking agent for collagen/chitosan scaffold in myocardial tissue engineering.
31530754	7	31	theme	crosslinking	1146:1157	arg1	agents					1159:1164	various crosslinking agents	1138:1164	various crosslinking agents	1138:1164	The collagen/chitosan scaffolds prepared with various crosslinking agents were fabricated and evaluated for physical characteristics, biocompatibility and contractile performance.
31530754	4	32	theme	cardiomyocytes	565:578	arg1	characteristics					546:560	the non-proliferative and contractile characteristics	508:560	the non-proliferative and contractile characteristics of cardiomyocytes	508:578	Due to the non-proliferative and contractile characteristics of cardiomyocytes, the biocompatibility and mechanical properties of the scaffolds are extremely important for supporting intercellular connection and tissue function for myocardial tissue engineering.
31530754	9	33	theme	excellent	1432:1440	arg1	properties					1453:1462	excellent mechanical properties	1432:1462	excellent mechanical properties	1432:1462	TPP crosslinked scaffolds showed excellent mechanical properties, with their elastic modulus (81.0 ± 8.1 kPa) in the physiological range of native myocardium (20∼100 kPa).
31530754	6	34	theme	tissue	1072:1077	arg1	engineering					1079:1089	myocardial tissue engineering	1061:1089	myocardial tissue engineering	1061:1089	Thus, the aim of this study is to compare and identify the crosslinking effect of GTA, GP and TPP for myocardial tissue engineering.
31530754	10	35	theme	crosslinked	1592:1602	arg1	scaffolds					1604:1612	GP and TPP crosslinked scaffolds	1581:1612	GP and TPP crosslinked scaffolds	1581:1612	Moreover, GP and TPP crosslinked scaffolds exhibited better biocompatibility than GTA crosslinked scaffolds, as demonstrated by the live/dead staining and proliferation assay.
31530754	7	36	theme	collagen/chitosan	1096:1112	arg1	scaffolds					1114:1122	The collagen/chitosan scaffolds	1092:1122	The collagen/chitosan scaffolds prepared with various crosslinking agents	1092:1164	The collagen/chitosan scaffolds prepared with various crosslinking agents were fabricated and evaluated for physical characteristics, biocompatibility and contractile performance.
31530754	12	37	theme	tissue	2063:2068	arg1	engineering					2070:2080	myocardial tissue engineering	2052:2080	myocardial tissue engineering	2052:2080	To conclude, of the three crosslinking agents, TPP was recommended as the most suitable crosslinking agent for collagen/chitosan scaffold in myocardial tissue engineering.
31530754	9	38	theme	elastic	1476:1482	arg1	modulus					1484:1490	their elastic modulus	1470:1490	their elastic modulus (81.0 ± 8.1 kPa) in the physiological range of native myocardium (20∼100 kPa)	1470:1568	TPP crosslinked scaffolds showed excellent mechanical properties, with their elastic modulus (81.0 ± 8.1 kPa) in the physiological range of native myocardium (20∼100 kPa).
31530754	9	38	theme	elastic	1476:1482	arg1	kPa					1504:1506	81.0 ± 8.1 kPa	1493:1506	81.0 ± 8.1 kPa	1493:1506	TPP crosslinked scaffolds showed excellent mechanical properties, with their elastic modulus (81.0 ± 8.1 kPa) in the physiological range of native myocardium (20∼100 kPa).
31530754	4	39	theme	tissue	713:718	arg1	function					720:727	tissue function	713:727	tissue function for myocardial tissue engineering	713:761	Due to the non-proliferative and contractile characteristics of cardiomyocytes, the biocompatibility and mechanical properties of the scaffolds are extremely important for supporting intercellular connection and tissue function for myocardial tissue engineering.
31530754	7	40	theme	contractile	1247:1257	arg1	performance					1259:1269	contractile performance	1247:1269	contractile performance	1247:1269	The collagen/chitosan scaffolds prepared with various crosslinking agents were fabricated and evaluated for physical characteristics, biocompatibility and contractile performance.
31530754	12	41	theme	collagen/chitosan	2022:2038	arg1	scaffold					2040:2047	collagen/chitosan scaffold	2022:2047	collagen/chitosan scaffold in myocardial tissue engineering	2022:2080	To conclude, of the three crosslinking agents, TPP was recommended as the most suitable crosslinking agent for collagen/chitosan scaffold in myocardial tissue engineering.
31530754	6	42	theme	study	981:985	arg1	aim					969:971	the aim	965:971	the aim of this study	965:985	Thus, the aim of this study is to compare and identify the crosslinking effect of GTA, GP and TPP for myocardial tissue engineering.
31530754	10	43	link	crosslinked	1657:1667	arg1	scaffolds					1669:1677	GTA crosslinked scaffolds	1653:1677	GTA crosslinked scaffolds	1653:1677	Moreover, GP and TPP crosslinked scaffolds exhibited better biocompatibility than GTA crosslinked scaffolds, as demonstrated by the live/dead staining and proliferation assay.
31530754	8	44	theme	scaffolds	1290:1298	arg1	groups					1280:1285	All the groups	1272:1285	All the groups of scaffolds	1272:1298	All the groups of scaffolds exhibited high porosity (>65%) and good swelling ratio suitable for myocardial tissue engineering.
31530754	8	44	theme	scaffolds	1290:1298	arg1	scaffolds					1290:1298	scaffolds	1290:1298	scaffolds	1290:1298	All the groups of scaffolds exhibited high porosity (>65%) and good swelling ratio suitable for myocardial tissue engineering.
31530754	11	45	theme	marker	1858:1863	arg1	protein					1865:1871	cardiac-specific marker protein	1841:1871	cardiac-specific marker protein	1841:1871	In addition, cardiomyocytes within TPP crosslinked scaffolds showed the highest expression of cardiac-specific marker protein and the best contractile performance.
31530754	12	46	theme	suitable	1990:1997	arg1	agent					2012:2016	the most suitable crosslinking agent	1981:2016	the most suitable crosslinking agent	1981:2016	To conclude, of the three crosslinking agents, TPP was recommended as the most suitable crosslinking agent for collagen/chitosan scaffold in myocardial tissue engineering.
31530754	5	47	dep	agents	817:822	arg1	GTA					841:843	GTA	841:843	GTA	841:843	To the best of our knowledge, the three crosslinking agents (glutaraldehyde (GTA), genipin (GP), tripolyphosphate (TPP)) have not yet been comparatively studied in myocardial tissue engineering.
31530754	5	47	dep	agents	817:822	arg1	tripolyphosphate					861:876	tripolyphosphate	861:876	tripolyphosphate (TPP)	861:882	To the best of our knowledge, the three crosslinking agents (glutaraldehyde (GTA), genipin (GP), tripolyphosphate (TPP)) have not yet been comparatively studied in myocardial tissue engineering.
31530754	5	47	dep	agents	817:822	arg1	genipin					847:853	genipin	847:853	genipin (GP)	847:858	To the best of our knowledge, the three crosslinking agents (glutaraldehyde (GTA), genipin (GP), tripolyphosphate (TPP)) have not yet been comparatively studied in myocardial tissue engineering.
31530754	5	47	dep	agents	817:822	arg1	glutaraldehyde					825:838	glutaraldehyde	825:838	glutaraldehyde (GTA)	825:844	To the best of our knowledge, the three crosslinking agents (glutaraldehyde (GTA), genipin (GP), tripolyphosphate (TPP)) have not yet been comparatively studied in myocardial tissue engineering.
31530754	8	48	theme	high	1310:1313	arg1	%					1328:1328	>65%	1325:1328	>65%	1325:1328	All the groups of scaffolds exhibited high porosity (>65%) and good swelling ratio suitable for myocardial tissue engineering.
31530754	8	48	theme	high	1310:1313	arg1	porosity					1315:1322	high porosity	1310:1322	high porosity (>65%)	1310:1329	All the groups of scaffolds exhibited high porosity (>65%) and good swelling ratio suitable for myocardial tissue engineering.
31530754	10	49	theme	GTA	1653:1655	arg1	scaffolds					1669:1677	GTA crosslinked scaffolds	1653:1677	GTA crosslinked scaffolds	1653:1677	Moreover, GP and TPP crosslinked scaffolds exhibited better biocompatibility than GTA crosslinked scaffolds, as demonstrated by the live/dead staining and proliferation assay.
31530754	1	50	from	challenge	235:243	arg1	engineering					266:276	myocardial tissue engineering	248:276	myocardial tissue engineering	248:276	Suitable material for scaffolds that support cell attachment, proliferation, vascularization and contraction has always been a challenge in myocardial tissue engineering.
31530754	9	51	theme	physiological	1516:1528	arg1	range					1530:1534	the physiological range	1512:1534	the physiological range of native myocardium (20∼100 kPa)	1512:1568	TPP crosslinked scaffolds showed excellent mechanical properties, with their elastic modulus (81.0 ± 8.1 kPa) in the physiological range of native myocardium (20∼100 kPa).
31530754	11	52	theme	best	1881:1884	arg1	performance					1898:1908	the best contractile performance	1877:1908	the best contractile performance	1877:1908	In addition, cardiomyocytes within TPP crosslinked scaffolds showed the highest expression of cardiac-specific marker protein and the best contractile performance.
31530754	5	53	theme	tissue	939:944	arg1	engineering					946:956	myocardial tissue engineering	928:956	myocardial tissue engineering	928:956	To the best of our knowledge, the three crosslinking agents (glutaraldehyde (GTA), genipin (GP), tripolyphosphate (TPP)) have not yet been comparatively studied in myocardial tissue engineering.
31530754	5	54	theme	crosslinking	804:815	arg1	agents					817:822	the three crosslinking agents	794:822	the three crosslinking agents (glutaraldehyde (GTA), genipin (GP), tripolyphosphate (TPP))	794:883	To the best of our knowledge, the three crosslinking agents (glutaraldehyde (GTA), genipin (GP), tripolyphosphate (TPP)) have not yet been comparatively studied in myocardial tissue engineering.
31530754	2	55	theme	Much	279:282	arg1	effort					293:298	Much research effort	279:298	Much research effort	279:298	Much research effort has been focused on natural polymers including collagen, gelatin, chitosan, fibrin, alginate, etc.
31530754	9	56	theme	myocardium	1546:1555	arg1	range					1530:1534	the physiological range	1512:1534	the physiological range of native myocardium (20∼100 kPa)	1512:1568	TPP crosslinked scaffolds showed excellent mechanical properties, with their elastic modulus (81.0 ± 8.1 kPa) in the physiological range of native myocardium (20∼100 kPa).
31530754	4	57	theme	non-proliferative	512:528	arg1	characteristics					546:560	the non-proliferative and contractile characteristics	508:560	the non-proliferative and contractile characteristics of cardiomyocytes	508:578	Due to the non-proliferative and contractile characteristics of cardiomyocytes, the biocompatibility and mechanical properties of the scaffolds are extremely important for supporting intercellular connection and tissue function for myocardial tissue engineering.
31530754	4	58	theme	tissue	744:749	arg1	engineering					751:761	myocardial tissue engineering	733:761	myocardial tissue engineering	733:761	Due to the non-proliferative and contractile characteristics of cardiomyocytes, the biocompatibility and mechanical properties of the scaffolds are extremely important for supporting intercellular connection and tissue function for myocardial tissue engineering.
31530754	11	59	theme	crosslinked	1786:1796	arg1	scaffolds					1798:1806	TPP crosslinked scaffolds	1782:1806	TPP crosslinked scaffolds	1782:1806	In addition, cardiomyocytes within TPP crosslinked scaffolds showed the highest expression of cardiac-specific marker protein and the best contractile performance.
31530754	4	60	theme	contractile	534:544	arg1	characteristics					546:560	the non-proliferative and contractile characteristics	508:560	the non-proliferative and contractile characteristics of cardiomyocytes	508:578	Due to the non-proliferative and contractile characteristics of cardiomyocytes, the biocompatibility and mechanical properties of the scaffolds are extremely important for supporting intercellular connection and tissue function for myocardial tissue engineering.
31530754	8	61	theme	swelling	1340:1347	arg1	ratio					1349:1353	good swelling ratio	1335:1353	good swelling ratio suitable for myocardial tissue engineering	1335:1396	All the groups of scaffolds exhibited high porosity (>65%) and good swelling ratio suitable for myocardial tissue engineering.
31530754	1	62	dep	cell	153:156	arg1	attachment					158:167	attachment	158:167	attachment	158:167	Suitable material for scaffolds that support cell attachment, proliferation, vascularization and contraction has always been a challenge in myocardial tissue engineering.
31530754	8	63	theme	suitable	1355:1362	arg1	ratio					1349:1353	good swelling ratio	1335:1353	good swelling ratio suitable for myocardial tissue engineering	1335:1396	All the groups of scaffolds exhibited high porosity (>65%) and good swelling ratio suitable for myocardial tissue engineering.
31530754	11	64	theme	highest	1819:1825	arg1	expression					1827:1836	the highest expression	1815:1836	the highest expression of cardiac-specific marker protein	1815:1871	In addition, cardiomyocytes within TPP crosslinked scaffolds showed the highest expression of cardiac-specific marker protein and the best contractile performance.
31530754	7	65	theme	physical	1200:1207	arg1	characteristics					1209:1223	physical characteristics	1200:1223	physical characteristics	1200:1223	The collagen/chitosan scaffolds prepared with various crosslinking agents were fabricated and evaluated for physical characteristics, biocompatibility and contractile performance.
31530754	6	66	theme	myocardial	1061:1070	arg1	engineering					1079:1089	myocardial tissue engineering	1061:1089	myocardial tissue engineering	1061:1089	Thus, the aim of this study is to compare and identify the crosslinking effect of GTA, GP and TPP for myocardial tissue engineering.
31530754	8	67	theme	myocardial	1368:1377	arg1	engineering					1386:1396	myocardial tissue engineering	1368:1396	myocardial tissue engineering	1368:1396	All the groups of scaffolds exhibited high porosity (>65%) and good swelling ratio suitable for myocardial tissue engineering.
31530754	6	68	theme	TPP	1053:1055	arg1	effect					1031:1036	the crosslinking effect	1014:1036	the crosslinking effect of GTA, GP and TPP for myocardial tissue engineering	1014:1089	Thus, the aim of this study is to compare and identify the crosslinking effect of GTA, GP and TPP for myocardial tissue engineering.
31530754	4	69	theme	biocompatibility	585:600	arg1	properties					617:626	the biocompatibility and mechanical properties	581:626	the biocompatibility and mechanical properties of the scaffolds	581:643	Due to the non-proliferative and contractile characteristics of cardiomyocytes, the biocompatibility and mechanical properties of the scaffolds are extremely important for supporting intercellular connection and tissue function for myocardial tissue engineering.
31530754	4	69	theme	biocompatibility	585:600	arg1	important					659:667	important	659:667	important	659:667	Due to the non-proliferative and contractile characteristics of cardiomyocytes, the biocompatibility and mechanical properties of the scaffolds are extremely important for supporting intercellular connection and tissue function for myocardial tissue engineering.
31530754	3	70	used	used	461:464	arg2	scaffold					441:448	a collagen/chitosan composite scaffold	411:448	a collagen/chitosan composite scaffold	411:448	Among them, a collagen/chitosan composite scaffold was widely used for myocardial tissue engineering.
31530754	0	71	theme	crosslinking	22:33	arg1	agents					35:40	various crosslinking agents	14:40	various crosslinking agents	14:40	Assessment of various crosslinking agents on collagen/chitosan scaffolds for myocardial tissue engineering.
31530754	6	72	theme	GP	1046:1047	arg1	effect					1031:1036	the crosslinking effect	1014:1036	the crosslinking effect of GTA, GP and TPP for myocardial tissue engineering	1014:1089	Thus, the aim of this study is to compare and identify the crosslinking effect of GTA, GP and TPP for myocardial tissue engineering.
31530754	4	73	theme	mechanical	606:615	arg1	properties					617:626	the biocompatibility and mechanical properties	581:626	the biocompatibility and mechanical properties of the scaffolds	581:643	Due to the non-proliferative and contractile characteristics of cardiomyocytes, the biocompatibility and mechanical properties of the scaffolds are extremely important for supporting intercellular connection and tissue function for myocardial tissue engineering.
31530754	4	73	theme	mechanical	606:615	arg1	important					659:667	important	659:667	important	659:667	Due to the non-proliferative and contractile characteristics of cardiomyocytes, the biocompatibility and mechanical properties of the scaffolds are extremely important for supporting intercellular connection and tissue function for myocardial tissue engineering.
31530754	3	74	theme	tissue	481:486	arg1	engineering					488:498	myocardial tissue engineering	470:498	myocardial tissue engineering	470:498	Among them, a collagen/chitosan composite scaffold was widely used for myocardial tissue engineering.
31530754	12	75	theme	crosslinking	1937:1948	arg1	agents					1950:1955	the three crosslinking agents	1927:1955	the three crosslinking agents	1927:1955	To conclude, of the three crosslinking agents, TPP was recommended as the most suitable crosslinking agent for collagen/chitosan scaffold in myocardial tissue engineering.
31530754	6	76	theme	GTA	1041:1043	arg1	effect					1031:1036	the crosslinking effect	1014:1036	the crosslinking effect of GTA, GP and TPP for myocardial tissue engineering	1014:1089	Thus, the aim of this study is to compare and identify the crosslinking effect of GTA, GP and TPP for myocardial tissue engineering.
31530754	3	77	theme	collagen/chitosan	413:429	arg1	scaffold					441:448	a collagen/chitosan composite scaffold	411:448	a collagen/chitosan composite scaffold	411:448	Among them, a collagen/chitosan composite scaffold was widely used for myocardial tissue engineering.
31530754	1	78	theme	tissue	259:264	arg1	engineering					266:276	myocardial tissue engineering	248:276	myocardial tissue engineering	248:276	Suitable material for scaffolds that support cell attachment, proliferation, vascularization and contraction has always been a challenge in myocardial tissue engineering.
31530754	7	79	theme	various	1138:1144	arg1	agents					1159:1164	various crosslinking agents	1138:1164	various crosslinking agents	1138:1164	The collagen/chitosan scaffolds prepared with various crosslinking agents were fabricated and evaluated for physical characteristics, biocompatibility and contractile performance.
31530754	9	80	link	crosslinked	1403:1413	arg1	scaffolds					1415:1423	TPP crosslinked scaffolds	1399:1423	TPP crosslinked scaffolds	1399:1423	TPP crosslinked scaffolds showed excellent mechanical properties, with their elastic modulus (81.0 ± 8.1 kPa) in the physiological range of native myocardium (20∼100 kPa).
31530754	9	81	theme	crosslinked	1403:1413	arg1	scaffolds					1415:1423	TPP crosslinked scaffolds	1399:1423	TPP crosslinked scaffolds	1399:1423	TPP crosslinked scaffolds showed excellent mechanical properties, with their elastic modulus (81.0 ± 8.1 kPa) in the physiological range of native myocardium (20∼100 kPa).
31530754	10	82	theme	proliferation	1726:1738	arg1	assay					1740:1744	proliferation assay	1726:1744	proliferation assay	1726:1744	Moreover, GP and TPP crosslinked scaffolds exhibited better biocompatibility than GTA crosslinked scaffolds, as demonstrated by the live/dead staining and proliferation assay.
31530754	1	83	theme	myocardial	248:257	arg1	engineering					266:276	myocardial tissue engineering	248:276	myocardial tissue engineering	248:276	Suitable material for scaffolds that support cell attachment, proliferation, vascularization and contraction has always been a challenge in myocardial tissue engineering.
31530754	10	84	link	crosslinked	1592:1602	arg1	scaffolds					1604:1612	GP and TPP crosslinked scaffolds	1581:1612	GP and TPP crosslinked scaffolds	1581:1612	Moreover, GP and TPP crosslinked scaffolds exhibited better biocompatibility than GTA crosslinked scaffolds, as demonstrated by the live/dead staining and proliferation assay.
31530754	9	85	theme	mechanical	1442:1451	arg1	properties					1453:1462	excellent mechanical properties	1432:1462	excellent mechanical properties	1432:1462	TPP crosslinked scaffolds showed excellent mechanical properties, with their elastic modulus (81.0 ± 8.1 kPa) in the physiological range of native myocardium (20∼100 kPa).
32122847	0	0	theme	escitalopram	79:90	arg1	film					71:74	oral fast dissolving thin film	45:74	oral fast dissolving thin film of escitalopram	45:90	Development and pharmaceutical evaluation of oral fast dissolving thin film of escitalopram: A patient friendly dosage form.
32122847	4	1	theme	casting	504:510	arg1	method					512:517	solvent casting method	496:517	solvent casting method incorporated with HPMC and PEG 400 as Plasticizer	496:567	The software generated six optimized formulations have been fabricated via solvent casting method incorporated with HPMC and PEG 400 as Plasticizer.
32122847	5	2	theme	drug-excipients	670:684	arg1	interaction					686:696	drug-excipients interaction	670:696	drug-excipients interaction	670:696	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	7	3	theme	ICH	1064:1066	arg1	guidelines					1098:1107	ICH accelerated stability studies guidelines	1064:1107	ICH accelerated stability studies guidelines	1064:1107	The formulations were subjected for stability studies for six months in accordance with ICH accelerated stability studies guidelines.
32122847	5	4	theme	percent	730:736	arg1	elongation					738:747	percent elongation	730:747	percent elongation	730:747	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	5	theme	surface	801:807	arg1	pH					809:810	surface pH	801:810	surface pH	801:810	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	10	6	theme	fast	1387:1390	arg1	films					1408:1412	oral fast dissolving thin films	1382:1412	oral fast dissolving thin films of escitalopram	1382:1428	The study concluded that a unique, novel, safe and stable formulation of oral fast dissolving thin films of escitalopram can be formulated with ease.
32122847	11	7	with	simple	1486:1491	arg1	tendency					1524:1531	scale-up tendency	1515:1531	scale-up tendency so that it can fulfill the need of the commercial manufacturing process	1515:1603	The preparation method was simple and reproducible with scale-up tendency so that it can fulfill the need of the commercial manufacturing process.
32122847	1	8	theme	Escitalopram	244:255	arg1	5mg					257:259	Escitalopram 5mg	244:259	Escitalopram 5mg	244:259	The current study focused on the development, optimization and pharmaceutical evaluation of a mouth dissolving film of Escitalopram 5mg.
32122847	5	9	theme	swelling	750:757	arg1	index					759:763	swelling index	750:763	swelling index	750:763	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	11	10	theme	manufacturing	1583:1595	arg1	process					1597:1603	the commercial manufacturing process	1568:1603	the commercial manufacturing process	1568:1603	The preparation method was simple and reproducible with scale-up tendency so that it can fulfill the need of the commercial manufacturing process.
32122847	5	11	theme	various	615:621	arg1	elongation					738:747	percent elongation	730:747	percent elongation	730:747	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	11	theme	various	615:621	arg1	uptake					849:854	moisture uptake	840:854	moisture uptake	840:854	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	11	theme	various	615:621	arg1	variation					659:667	weight variation	652:667	weight variation	652:667	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	11	theme	various	615:621	arg1	stability					857:865	stability	857:865	stability	857:865	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	11	theme	various	615:621	arg1	endurance					790:798	folding endurance	782:798	folding endurance	782:798	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	11	theme	various	615:621	arg1	test					713:716	dryness/ tack test	699:716	dryness/ tack test	699:716	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	11	theme	various	615:621	arg1	uniformity					821:830	content uniformity	813:830	content uniformity	813:830	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	11	theme	various	615:621	arg1	thickness					719:727	thickness	719:727	thickness	719:727	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	11	theme	various	615:621	arg1	assay					833:837	assay	833:837	assay	833:837	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	11	theme	various	615:621	arg1	index					759:763	swelling index	750:763	swelling index	750:763	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	11	theme	various	615:621	arg1	attributes					631:640	various quality attributes	615:640	various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties	615:894	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	11	theme	various	615:621	arg1	interaction					686:696	drug-excipients interaction	670:696	drug-excipients interaction	670:696	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	11	theme	various	615:621	arg1	disintegration					766:779	disintegration	766:779	disintegration	766:779	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	11	theme	various	615:621	arg1	pH					809:810	surface pH	801:810	surface pH	801:810	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	11	theme	various	615:621	arg1	properties					885:894	organoleptic properties	872:894	organoleptic properties	872:894	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	1	12	theme	5mg	257:259	arg1	film					236:239	a mouth dissolving film	217:239	a mouth dissolving film of Escitalopram 5mg	217:259	The current study focused on the development, optimization and pharmaceutical evaluation of a mouth dissolving film of Escitalopram 5mg.
32122847	10	13	theme	novel	1344:1348	arg1	formulation					1367:1377	a unique, novel, safe and stable formulation	1334:1377	a unique, novel, safe and stable formulation of oral fast dissolving thin films of escitalopram	1334:1428	The study concluded that a unique, novel, safe and stable formulation of oral fast dissolving thin films of escitalopram can be formulated with ease.
32122847	6	14	theme	spectrophotometric	909:926	arg1	method					928:933	A validated spectrophotometric method	897:933	A validated spectrophotometric method	897:933	A validated spectrophotometric method has been used to ascertain drug content.
32122847	5	15	theme	dryness/	699:706	arg1	test					713:716	dryness/ tack test	699:716	dryness/ tack test	699:716	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	16	theme	quality	623:629	arg1	elongation					738:747	percent elongation	730:747	percent elongation	730:747	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	16	theme	quality	623:629	arg1	uptake					849:854	moisture uptake	840:854	moisture uptake	840:854	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	16	theme	quality	623:629	arg1	variation					659:667	weight variation	652:667	weight variation	652:667	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	16	theme	quality	623:629	arg1	stability					857:865	stability	857:865	stability	857:865	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	16	theme	quality	623:629	arg1	endurance					790:798	folding endurance	782:798	folding endurance	782:798	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	16	theme	quality	623:629	arg1	test					713:716	dryness/ tack test	699:716	dryness/ tack test	699:716	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	16	theme	quality	623:629	arg1	uniformity					821:830	content uniformity	813:830	content uniformity	813:830	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	16	theme	quality	623:629	arg1	thickness					719:727	thickness	719:727	thickness	719:727	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	16	theme	quality	623:629	arg1	assay					833:837	assay	833:837	assay	833:837	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	16	theme	quality	623:629	arg1	index					759:763	swelling index	750:763	swelling index	750:763	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	16	theme	quality	623:629	arg1	attributes					631:640	various quality attributes	615:640	various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties	615:894	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	16	theme	quality	623:629	arg1	interaction					686:696	drug-excipients interaction	670:696	drug-excipients interaction	670:696	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	16	theme	quality	623:629	arg1	disintegration					766:779	disintegration	766:779	disintegration	766:779	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	16	theme	quality	623:629	arg1	pH					809:810	surface pH	801:810	surface pH	801:810	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	16	theme	quality	623:629	arg1	properties					885:894	organoleptic properties	872:894	organoleptic properties	872:894	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	10	17	theme	unique	1336:1341	arg1	formulation					1367:1377	a unique, novel, safe and stable formulation	1334:1377	a unique, novel, safe and stable formulation of oral fast dissolving thin films of escitalopram	1334:1428	The study concluded that a unique, novel, safe and stable formulation of oral fast dissolving thin films of escitalopram can be formulated with ease.
32122847	11	18	theme	commercial	1572:1581	arg1	process					1597:1603	the commercial manufacturing process	1568:1603	the commercial manufacturing process	1568:1603	The preparation method was simple and reproducible with scale-up tendency so that it can fulfill the need of the commercial manufacturing process.
32122847	9	19	dep	in	1198:1199	arg1	vitro					1201:1205	vitro	1201:1205	vitro	1201:1205	All the test formulations have shown satisfactory in vitro release of escitalopram whereas most promising results have been exhibited by F5 and F6 formulations.
32122847	0	20	theme	friendly	103:110	arg1	form					119:122	A patient friendly dosage form	93:122	Development and pharmaceutical evaluation of oral fast dissolving thin film of escitalopram: A patient friendly dosage form.	0:123	Development and pharmaceutical evaluation of oral fast dissolving thin film of escitalopram: A patient friendly dosage form.
32122847	6	21	theme	validated	899:907	arg1	method					928:933	A validated spectrophotometric method	897:933	A validated spectrophotometric method	897:933	A validated spectrophotometric method has been used to ascertain drug content.
32122847	10	22	theme	dissolving	1392:1401	arg1	films					1408:1412	oral fast dissolving thin films	1382:1412	oral fast dissolving thin films of escitalopram	1382:1428	The study concluded that a unique, novel, safe and stable formulation of oral fast dissolving thin films of escitalopram can be formulated with ease.
32122847	0	23	theme	patient	95:101	arg1	form					119:122	A patient friendly dosage form	93:122	Development and pharmaceutical evaluation of oral fast dissolving thin film of escitalopram: A patient friendly dosage form.	0:123	Development and pharmaceutical evaluation of oral fast dissolving thin film of escitalopram: A patient friendly dosage form.
32122847	4	24	theme	solvent	496:502	arg1	method					512:517	solvent casting method	496:517	solvent casting method incorporated with HPMC and PEG 400 as Plasticizer	496:567	The software generated six optimized formulations have been fabricated via solvent casting method incorporated with HPMC and PEG 400 as Plasticizer.
32122847	3	25	theme	surface	400:406	arg1	methodology					408:418	central composite response surface methodology	373:418	central composite response surface methodology	373:418	9, using central composite response surface methodology.
32122847	5	26	theme	developed	574:582	arg1	formulations					584:595	The developed formulations	570:595	The developed formulations	570:595	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	9	27	dep	F5	1285:1286	arg1	formulations					1295:1306	formulations	1295:1306	formulations	1295:1306	All the test formulations have shown satisfactory in vitro release of escitalopram whereas most promising results have been exhibited by F5 and F6 formulations.
32122847	11	28	theme	process	1597:1603	arg1	need					1560:1563	the need	1556:1563	the need of the commercial manufacturing process	1556:1603	The preparation method was simple and reproducible with scale-up tendency so that it can fulfill the need of the commercial manufacturing process.
32122847	11	29	theme	preparation	1463:1473	arg1	method					1475:1480	The preparation method	1459:1480	The preparation method	1459:1480	The preparation method was simple and reproducible with scale-up tendency so that it can fulfill the need of the commercial manufacturing process.
32122847	11	29	theme	preparation	1463:1473	arg1	simple					1486:1491	simple	1486:1491	simple	1486:1491	The preparation method was simple and reproducible with scale-up tendency so that it can fulfill the need of the commercial manufacturing process.
32122847	9	30	theme	promising	1244:1252	arg1	results					1254:1260	most promising results	1239:1260	most promising results	1239:1260	All the test formulations have shown satisfactory in vitro release of escitalopram whereas most promising results have been exhibited by F5 and F6 formulations.
32122847	7	31	theme	accelerated	1068:1078	arg1	guidelines					1098:1107	ICH accelerated stability studies guidelines	1064:1107	ICH accelerated stability studies guidelines	1064:1107	The formulations were subjected for stability studies for six months in accordance with ICH accelerated stability studies guidelines.
32122847	5	32	theme	organoleptic	872:883	arg1	properties					885:894	organoleptic properties	872:894	organoleptic properties	872:894	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	3	33	theme	central	373:379	arg1	methodology					408:418	central composite response surface methodology	373:418	central composite response surface methodology	373:418	9, using central composite response surface methodology.
32122847	2	34	theme	formulations	300:311	arg1	designing					266:274	designing	266:274	designing	266:274	The designing and optimization of the formulations have been carried out through Design-Expert ⌖ Ver.
32122847	0	35	theme	fast	50:53	arg1	film					71:74	oral fast dissolving thin film	45:74	oral fast dissolving thin film of escitalopram	45:90	Development and pharmaceutical evaluation of oral fast dissolving thin film of escitalopram: A patient friendly dosage form.
32122847	8	36	theme	stability	1113:1121	arg1	issue					1123:1127	No stability issue	1110:1127	No stability issue	1110:1127	No stability issue has been observed.
32122847	10	37	theme	escitalopram	1417:1428	arg1	films					1408:1412	oral fast dissolving thin films	1382:1412	oral fast dissolving thin films of escitalopram	1382:1428	The study concluded that a unique, novel, safe and stable formulation of oral fast dissolving thin films of escitalopram can be formulated with ease.
32122847	5	38	theme	moisture	840:847	arg1	uptake					849:854	moisture uptake	840:854	moisture uptake	840:854	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	0	39	theme	pharmaceutical	16:29	arg1	evaluation					31:40	pharmaceutical evaluation	16:40	pharmaceutical evaluation	16:40	Development and pharmaceutical evaluation of oral fast dissolving thin film of escitalopram: A patient friendly dosage form.
32122847	0	40	theme	dosage	112:117	arg1	form					119:122	A patient friendly dosage form	93:122	Development and pharmaceutical evaluation of oral fast dissolving thin film of escitalopram: A patient friendly dosage form.	0:123	Development and pharmaceutical evaluation of oral fast dissolving thin film of escitalopram: A patient friendly dosage form.
32122847	4	41	theme	optimized	448:456	arg1	formulations					458:469	six optimized formulations	444:469	six optimized formulations	444:469	The software generated six optimized formulations have been fabricated via solvent casting method incorporated with HPMC and PEG 400 as Plasticizer.
32122847	9	42	theme	escitalopram	1218:1229	arg1	release					1207:1213	satisfactory in vitro release	1185:1213	satisfactory in vitro release of escitalopram	1185:1229	All the test formulations have shown satisfactory in vitro release of escitalopram whereas most promising results have been exhibited by F5 and F6 formulations.
32122847	5	43	theme	content	813:819	arg1	uniformity					821:830	content uniformity	813:830	content uniformity	813:830	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	1	44	theme	pharmaceutical	188:201	arg1	evaluation					203:212	pharmaceutical evaluation	188:212	pharmaceutical evaluation	188:212	The current study focused on the development, optimization and pharmaceutical evaluation of a mouth dissolving film of Escitalopram 5mg.
32122847	0	45	theme	oral	45:48	arg1	film					71:74	oral fast dissolving thin film	45:74	oral fast dissolving thin film of escitalopram	45:90	Development and pharmaceutical evaluation of oral fast dissolving thin film of escitalopram: A patient friendly dosage form.
32122847	7	46	theme	stability	1080:1088	arg1	guidelines					1098:1107	ICH accelerated stability studies guidelines	1064:1107	ICH accelerated stability studies guidelines	1064:1107	The formulations were subjected for stability studies for six months in accordance with ICH accelerated stability studies guidelines.
32122847	7	47	theme	studies	1090:1096	arg1	guidelines					1098:1107	ICH accelerated stability studies guidelines	1064:1107	ICH accelerated stability studies guidelines	1064:1107	The formulations were subjected for stability studies for six months in accordance with ICH accelerated stability studies guidelines.
32122847	10	48	theme	stable	1360:1365	arg1	formulation					1367:1377	a unique, novel, safe and stable formulation	1334:1377	a unique, novel, safe and stable formulation of oral fast dissolving thin films of escitalopram	1334:1428	The study concluded that a unique, novel, safe and stable formulation of oral fast dissolving thin films of escitalopram can be formulated with ease.
32122847	6	49	used	used	944:947	arg2	method					928:933	A validated spectrophotometric method	897:933	A validated spectrophotometric method	897:933	A validated spectrophotometric method has been used to ascertain drug content.
32122847	0	50	theme	dissolving	55:64	arg1	film					71:74	oral fast dissolving thin film	45:74	oral fast dissolving thin film of escitalopram	45:90	Development and pharmaceutical evaluation of oral fast dissolving thin film of escitalopram: A patient friendly dosage form.
32122847	6	51	theme	drug	962:965	arg1	content					967:973	drug content	962:973	drug content	962:973	A validated spectrophotometric method has been used to ascertain drug content.
32122847	5	52	theme	tack	708:711	arg1	test					713:716	dryness/ tack test	699:716	dryness/ tack test	699:716	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	5	53	theme	folding	782:788	arg1	endurance					790:798	folding endurance	782:798	folding endurance	782:798	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	9	54	theme	test	1156:1159	arg1	formulations					1161:1172	All the test formulations	1148:1172	All the test formulations	1148:1172	All the test formulations have shown satisfactory in vitro release of escitalopram whereas most promising results have been exhibited by F5 and F6 formulations.
32122847	3	55	theme	response	391:398	arg1	methodology					408:418	central composite response surface methodology	373:418	central composite response surface methodology	373:418	9, using central composite response surface methodology.
32122847	2	56	dep	designing	266:274	arg1	The					262:264	The	262:264	The	262:264	The designing and optimization of the formulations have been carried out through Design-Expert ⌖ Ver.
32122847	5	57	theme	weight	652:657	arg1	variation					659:667	weight variation	652:667	weight variation	652:667	The developed formulations were assessed for various quality attributes including weight variation, drug-excipients interaction, dryness/ tack test, thickness, percent elongation, swelling index, disintegration, folding endurance, surface pH, content uniformity, assay, moisture uptake, stability, and organoleptic properties.
32122847	10	58	theme	safe	1351:1354	arg1	formulation					1367:1377	a unique, novel, safe and stable formulation	1334:1377	a unique, novel, safe and stable formulation of oral fast dissolving thin films of escitalopram	1334:1428	The study concluded that a unique, novel, safe and stable formulation of oral fast dissolving thin films of escitalopram can be formulated with ease.
32122847	9	59	theme	most	1239:1242	arg1	results					1254:1260	most promising results	1239:1260	most promising results	1239:1260	All the test formulations have shown satisfactory in vitro release of escitalopram whereas most promising results have been exhibited by F5 and F6 formulations.
32122847	3	60	theme	composite	381:389	arg1	methodology					408:418	central composite response surface methodology	373:418	central composite response surface methodology	373:418	9, using central composite response surface methodology.
32122847	10	61	theme	films	1408:1412	arg1	formulation					1367:1377	a unique, novel, safe and stable formulation	1334:1377	a unique, novel, safe and stable formulation of oral fast dissolving thin films of escitalopram	1334:1428	The study concluded that a unique, novel, safe and stable formulation of oral fast dissolving thin films of escitalopram can be formulated with ease.
32122847	10	62	theme	thin	1403:1406	arg1	films					1408:1412	oral fast dissolving thin films	1382:1412	oral fast dissolving thin films of escitalopram	1382:1428	The study concluded that a unique, novel, safe and stable formulation of oral fast dissolving thin films of escitalopram can be formulated with ease.
32122847	11	63	theme	scale-up	1515:1522	arg1	tendency					1524:1531	scale-up tendency	1515:1531	scale-up tendency so that it can fulfill the need of the commercial manufacturing process	1515:1603	The preparation method was simple and reproducible with scale-up tendency so that it can fulfill the need of the commercial manufacturing process.
32122847	7	64	theme	stability	1012:1020	arg1	studies					1022:1028	stability studies	1012:1028	stability studies for six months in accordance with ICH accelerated stability studies guidelines	1012:1107	The formulations were subjected for stability studies for six months in accordance with ICH accelerated stability studies guidelines.
32122847	1	65	theme	mouth	219:223	arg1	film					236:239	a mouth dissolving film	217:239	a mouth dissolving film of Escitalopram 5mg	217:259	The current study focused on the development, optimization and pharmaceutical evaluation of a mouth dissolving film of Escitalopram 5mg.
32122847	0	66	theme	film	71:74	arg1	Development					0:10	Development	0:10	Development	0:10	Development and pharmaceutical evaluation of oral fast dissolving thin film of escitalopram: A patient friendly dosage form.
32122847	0	66	theme	film	71:74	arg1	evaluation					31:40	pharmaceutical evaluation	16:40	pharmaceutical evaluation	16:40	Development and pharmaceutical evaluation of oral fast dissolving thin film of escitalopram: A patient friendly dosage form.
32122847	10	67	theme	oral	1382:1385	arg1	films					1408:1412	oral fast dissolving thin films	1382:1412	oral fast dissolving thin films of escitalopram	1382:1428	The study concluded that a unique, novel, safe and stable formulation of oral fast dissolving thin films of escitalopram can be formulated with ease.
32122847	9	68	theme	satisfactory	1185:1196	arg1	release					1207:1213	satisfactory in vitro release	1185:1213	satisfactory in vitro release of escitalopram	1185:1229	All the test formulations have shown satisfactory in vitro release of escitalopram whereas most promising results have been exhibited by F5 and F6 formulations.
32122847	1	69	theme	current	129:135	arg1	study					137:141	The current study	125:141	The current study	125:141	The current study focused on the development, optimization and pharmaceutical evaluation of a mouth dissolving film of Escitalopram 5mg.
32122847	1	70	theme	dissolving	225:234	arg1	film					236:239	a mouth dissolving film	217:239	a mouth dissolving film of Escitalopram 5mg	217:259	The current study focused on the development, optimization and pharmaceutical evaluation of a mouth dissolving film of Escitalopram 5mg.
32122847	0	71	theme	thin	66:69	arg1	film					71:74	oral fast dissolving thin film	45:74	oral fast dissolving thin film of escitalopram	45:90	Development and pharmaceutical evaluation of oral fast dissolving thin film of escitalopram: A patient friendly dosage form.
32122847	0	72	dep	Development	0:10	arg1	form					119:122	A patient friendly dosage form	93:122	Development and pharmaceutical evaluation of oral fast dissolving thin film of escitalopram: A patient friendly dosage form.	0:123	Development and pharmaceutical evaluation of oral fast dissolving thin film of escitalopram: A patient friendly dosage form.
32122847	9	73	theme	in	1198:1199	arg1	release					1207:1213	satisfactory in vitro release	1185:1213	satisfactory in vitro release of escitalopram	1185:1229	All the test formulations have shown satisfactory in vitro release of escitalopram whereas most promising results have been exhibited by F5 and F6 formulations.
32122847	1	74	theme	film	236:239	arg1	optimization					171:182	optimization	171:182	optimization	171:182	The current study focused on the development, optimization and pharmaceutical evaluation of a mouth dissolving film of Escitalopram 5mg.
32122847	1	74	theme	film	236:239	arg1	evaluation					203:212	pharmaceutical evaluation	188:212	pharmaceutical evaluation	188:212	The current study focused on the development, optimization and pharmaceutical evaluation of a mouth dissolving film of Escitalopram 5mg.
32122847	1	74	theme	film	236:239	arg1	development					158:168	development	158:168	development	158:168	The current study focused on the development, optimization and pharmaceutical evaluation of a mouth dissolving film of Escitalopram 5mg.
32239052	4	0	contain	have	733:736	arg2	toxicity					759:766	unique and undefined toxicity	738:766	unique and undefined toxicity to Saccharomyces cerevisiae that is different from CuSO4	738:823	The carboxymethyl cellulose copper nanoparticles (CMC-Cu) investigated in this study have unique and undefined toxicity to Saccharomyces cerevisiae that is different from CuSO4.
32239052	4	0	contain	have	733:736	arg1	CMC-Cu					698:703	CMC-Cu	698:703	CMC-Cu	698:703	The carboxymethyl cellulose copper nanoparticles (CMC-Cu) investigated in this study have unique and undefined toxicity to Saccharomyces cerevisiae that is different from CuSO4.
32239052	4	0	contain	have	733:736	arg1	nanoparticles					683:695	The carboxymethyl cellulose copper nanoparticles	648:695	The carboxymethyl cellulose copper nanoparticles (CMC-Cu) investigated in this study	648:731	The carboxymethyl cellulose copper nanoparticles (CMC-Cu) investigated in this study have unique and undefined toxicity to Saccharomyces cerevisiae that is different from CuSO4.
32239052	5	1	theme	electron	872:879	arg1	micrographs					881:891	scanning electron micrographs	863:891	scanning electron micrographs upon CMC-Cu exposure	863:912	Cellular surface damage was found in scanning electron micrographs upon CMC-Cu exposure.
32239052	3	2	theme	intrinsic	520:528	arg1	chemistry					540:548	the intrinsic elemental chemistry	516:548	the intrinsic elemental chemistry	516:548	Mass is the traditional measurement of the intrinsic elemental chemistry, but this practice fails to reflect the morphology and surface area reactivity of nanotechnology.
32239052	0	3	theme	copper	74:79	arg1	nanoparticles					81:93	cellulosic copper nanoparticles	63:93	cellulosic copper nanoparticles	63:93	Metallomic and lipidomic analysis of S. cerevisiae response to cellulosic copper nanoparticles uncovers drivers of toxicity.
32239052	9	4	theme	cellular	1387:1394	arg1	concentrations					1401:1414	cellular zinc concentrations	1387:1414	cellular zinc concentrations	1387:1414	The copper sensitive yeast strain, YJM789, significantly decreased cellular zinc concentrations while the copper concentrations increased, suggesting a possible ionic mimicry relationship.
32239052	10	5	theme	genotoxicity	1574:1585	arg1	evidence					1562:1569	no evidence	1559:1569	no evidence of genotoxicity	1559:1585	In contrast to other compounds that generate ROS, no evidence of genotoxicity was found.
32239052	4	6	from	CuSO4	819:823	arg1	different					804:812	different	804:812	different	804:812	The carboxymethyl cellulose copper nanoparticles (CMC-Cu) investigated in this study have unique and undefined toxicity to Saccharomyces cerevisiae that is different from CuSO4.
32239052	3	7	theme	elemental	530:538	arg1	chemistry					540:548	the intrinsic elemental chemistry	516:548	the intrinsic elemental chemistry	516:548	Mass is the traditional measurement of the intrinsic elemental chemistry, but this practice fails to reflect the morphology and surface area reactivity of nanotechnology.
32239052	1	8	theme	food	263:266	arg1	applications					278:289	medical and food packaging applications	251:289	medical and food packaging applications	251:289	Nanotechnology is a promising new technology, of which antimicrobial metal nanocomposites are predicted to become valuable in medical and food packaging applications.
32239052	9	9	theme	copper	1324:1329	arg1	YJM789					1355:1360	YJM789	1355:1360	YJM789	1355:1360	The copper sensitive yeast strain, YJM789, significantly decreased cellular zinc concentrations while the copper concentrations increased, suggesting a possible ionic mimicry relationship.
32239052	9	9	theme	copper	1324:1329	arg1	strain					1347:1352	The copper sensitive yeast strain	1320:1352	The copper sensitive yeast strain	1320:1352	The copper sensitive yeast strain, YJM789, significantly decreased cellular zinc concentrations while the copper concentrations increased, suggesting a possible ionic mimicry relationship.
32239052	6	10	theme	altered	962:968	arg1	phosphatidylcholine					970:988	altered phosphatidylcholine	962:988	altered phosphatidylcholine	962:988	Further investigation into the lipids revealed altered phosphatidylcholine and phosphatidylethanolamine membrane composition, as well as depleted triacylglycerols, suggesting an impact on the Kennedy lipid pathway.
32239052	3	11	theme	chemistry	540:548	arg1	Mass					477:480	Mass	477:480	Mass	477:480	Mass is the traditional measurement of the intrinsic elemental chemistry, but this practice fails to reflect the morphology and surface area reactivity of nanotechnology.
32239052	3	11	theme	chemistry	540:548	arg1	measurement					501:511	the traditional measurement	485:511	the traditional measurement of the intrinsic elemental chemistry	485:548	Mass is the traditional measurement of the intrinsic elemental chemistry, but this practice fails to reflect the morphology and surface area reactivity of nanotechnology.
32239052	9	12	theme	mimicry	1487:1493	arg1	relationship					1495:1506	a possible ionic mimicry relationship	1470:1506	a possible ionic mimicry relationship	1470:1506	The copper sensitive yeast strain, YJM789, significantly decreased cellular zinc concentrations while the copper concentrations increased, suggesting a possible ionic mimicry relationship.
32239052	1	13	theme	packaging	268:276	arg1	applications					278:289	medical and food packaging applications	251:289	medical and food packaging applications	251:289	Nanotechnology is a promising new technology, of which antimicrobial metal nanocomposites are predicted to become valuable in medical and food packaging applications.
32239052	10	14	located	found	1591:1595	arg1	contrast					1512:1519	contrast	1512:1519	contrast to other compounds that generate ROS	1512:1556	In contrast to other compounds that generate ROS, no evidence of genotoxicity was found.
32239052	10	14	located	found	1591:1595	arg2	evidence					1562:1569	no evidence	1559:1569	no evidence of genotoxicity	1559:1585	In contrast to other compounds that generate ROS, no evidence of genotoxicity was found.
32239052	0	15	theme	toxicity	115:122	arg1	drivers					104:110	drivers	104:110	drivers of toxicity	104:122	Metallomic and lipidomic analysis of S. cerevisiae response to cellulosic copper nanoparticles uncovers drivers of toxicity.
32239052	7	16	theme	CMC-Cu	1250:1255	arg1	treatment					1257:1265	CMC-Cu treatment	1250:1265	CMC-Cu treatment	1250:1265	High levels of reactive oxygen species were measured which likely played a role in the lipid peroxidation detected with CMC-Cu treatment.
32239052	4	17	theme	carboxymethyl	652:664	arg1	CMC-Cu					698:703	CMC-Cu	698:703	CMC-Cu	698:703	The carboxymethyl cellulose copper nanoparticles (CMC-Cu) investigated in this study have unique and undefined toxicity to Saccharomyces cerevisiae that is different from CuSO4.
32239052	4	17	theme	carboxymethyl	652:664	arg1	nanoparticles					683:695	The carboxymethyl cellulose copper nanoparticles	648:695	The carboxymethyl cellulose copper nanoparticles (CMC-Cu) investigated in this study	648:731	The carboxymethyl cellulose copper nanoparticles (CMC-Cu) investigated in this study have unique and undefined toxicity to Saccharomyces cerevisiae that is different from CuSO4.
32239052	2	18	theme	atom	416:419	arg1	selectivity					421:431	the target biomolecule's donor atom selectivity	385:431	the target biomolecule's donor atom selectivity	385:431	Copper is a redox-active antimicrobial metal that can become increasingly toxic depending on the target biomolecule's donor atom selectivity and the chemical species of copper present.
32239052	8	19	theme	CMC-Cu	1302:1307	arg1	treatment					1309:1317	CMC-Cu treatment	1302:1317	CMC-Cu treatment	1302:1317	Metal homeostasis was affected by CMC-Cu treatment.
32239052	0	20	theme	Metallomic	0:9	arg1	analysis					25:32	Metallomic and lipidomic analysis	0:32	Metallomic and lipidomic analysis of S. cerevisiae response to cellulosic copper nanoparticles	0:93	Metallomic and lipidomic analysis of S. cerevisiae response to cellulosic copper nanoparticles uncovers drivers of toxicity.
32239052	2	21	theme	donor	410:414	arg1	selectivity					421:431	the target biomolecule's donor atom selectivity	385:431	the target biomolecule's donor atom selectivity	385:431	Copper is a redox-active antimicrobial metal that can become increasingly toxic depending on the target biomolecule's donor atom selectivity and the chemical species of copper present.
32239052	2	22	theme	present	468:474	arg1	species					450:456	the chemical species	437:456	the chemical species of copper present	437:474	Copper is a redox-active antimicrobial metal that can become increasingly toxic depending on the target biomolecule's donor atom selectivity and the chemical species of copper present.
32239052	2	22	theme	present	468:474	arg1	selectivity					421:431	the target biomolecule's donor atom selectivity	385:431	the target biomolecule's donor atom selectivity	385:431	Copper is a redox-active antimicrobial metal that can become increasingly toxic depending on the target biomolecule's donor atom selectivity and the chemical species of copper present.
32239052	8	23	theme	Metal	1268:1272	arg1	homeostasis					1274:1284	Metal homeostasis	1268:1284	Metal homeostasis	1268:1284	Metal homeostasis was affected by CMC-Cu treatment.
32239052	6	24	from	impact	1093:1098	arg1	pathway					1121:1127	the Kennedy lipid pathway	1103:1127	the Kennedy lipid pathway	1103:1127	Further investigation into the lipids revealed altered phosphatidylcholine and phosphatidylethanolamine membrane composition, as well as depleted triacylglycerols, suggesting an impact on the Kennedy lipid pathway.
32239052	11	25	theme	past	1692:1695	arg1	measurements					1709:1720	forward past traditional measurements	1684:1720	forward past traditional measurements of toxicity	1684:1732	As commonplace objects become more integrated with nanotechnology, humanity must look forward past traditional measurements of toxicity.
32239052	4	26	theme	Saccharomyces	771:783	arg1	cerevisiae					785:794	Saccharomyces cerevisiae	771:794	Saccharomyces cerevisiae	771:794	The carboxymethyl cellulose copper nanoparticles (CMC-Cu) investigated in this study have unique and undefined toxicity to Saccharomyces cerevisiae that is different from CuSO4.
32239052	1	27	theme	antimicrobial	180:192	arg1	nanocomposites					200:213	antimicrobial metal nanocomposites	180:213	antimicrobial metal nanocomposites	180:213	Nanotechnology is a promising new technology, of which antimicrobial metal nanocomposites are predicted to become valuable in medical and food packaging applications.
32239052	2	28	theme	copper	461:466	arg1	present					468:474	copper present	461:474	copper present	461:474	Copper is a redox-active antimicrobial metal that can become increasingly toxic depending on the target biomolecule's donor atom selectivity and the chemical species of copper present.
32239052	9	29	theme	copper	1426:1431	arg1	concentrations					1433:1446	the copper concentrations	1422:1446	the copper concentrations	1422:1446	The copper sensitive yeast strain, YJM789, significantly decreased cellular zinc concentrations while the copper concentrations increased, suggesting a possible ionic mimicry relationship.
32239052	10	30	theme	other	1524:1528	arg1	compounds					1530:1538	other compounds	1524:1538	other compounds that generate ROS	1524:1556	In contrast to other compounds that generate ROS, no evidence of genotoxicity was found.
32239052	1	31	theme	metal	194:198	arg1	nanocomposites					200:213	antimicrobial metal nanocomposites	180:213	antimicrobial metal nanocomposites	180:213	Nanotechnology is a promising new technology, of which antimicrobial metal nanocomposites are predicted to become valuable in medical and food packaging applications.
32239052	6	32	theme	depleted	1052:1059	arg1	triacylglycerols					1061:1076	depleted triacylglycerols	1052:1076	depleted triacylglycerols	1052:1076	Further investigation into the lipids revealed altered phosphatidylcholine and phosphatidylethanolamine membrane composition, as well as depleted triacylglycerols, suggesting an impact on the Kennedy lipid pathway.
32239052	7	33	theme	oxygen	1154:1159	arg1	species					1161:1167	reactive oxygen species	1145:1167	reactive oxygen species	1145:1167	High levels of reactive oxygen species were measured which likely played a role in the lipid peroxidation detected with CMC-Cu treatment.
32239052	2	34	theme	antimicrobial	317:329	arg1	metal					331:335	a redox-active antimicrobial metal	302:335	a redox-active antimicrobial metal that can become increasingly toxic depending on the target biomolecule's donor atom selectivity and the chemical species of copper present	302:474	Copper is a redox-active antimicrobial metal that can become increasingly toxic depending on the target biomolecule's donor atom selectivity and the chemical species of copper present.
32239052	2	34	theme	antimicrobial	317:329	arg1	Copper					292:297	Copper	292:297	Copper	292:297	Copper is a redox-active antimicrobial metal that can become increasingly toxic depending on the target biomolecule's donor atom selectivity and the chemical species of copper present.
32239052	5	35	located	found	854:858	arg2	damage					843:848	Cellular surface damage	826:848	Cellular surface damage	826:848	Cellular surface damage was found in scanning electron micrographs upon CMC-Cu exposure.
32239052	5	35	located	found	854:858	arg1	micrographs					881:891	scanning electron micrographs	863:891	scanning electron micrographs upon CMC-Cu exposure	863:912	Cellular surface damage was found in scanning electron micrographs upon CMC-Cu exposure.
32239052	2	36	theme	chemical	441:448	arg1	species					450:456	the chemical species	437:456	the chemical species of copper present	437:474	Copper is a redox-active antimicrobial metal that can become increasingly toxic depending on the target biomolecule's donor atom selectivity and the chemical species of copper present.
32239052	0	37	theme	lipidomic	15:23	arg1	analysis					25:32	Metallomic and lipidomic analysis	0:32	Metallomic and lipidomic analysis of S. cerevisiae response to cellulosic copper nanoparticles	0:93	Metallomic and lipidomic analysis of S. cerevisiae response to cellulosic copper nanoparticles uncovers drivers of toxicity.
32239052	7	38	theme	species	1161:1167	arg1	levels					1135:1140	High levels	1130:1140	High levels of reactive oxygen species	1130:1167	High levels of reactive oxygen species were measured which likely played a role in the lipid peroxidation detected with CMC-Cu treatment.
32239052	2	39	theme	redox-active	304:315	arg1	metal					331:335	a redox-active antimicrobial metal	302:335	a redox-active antimicrobial metal that can become increasingly toxic depending on the target biomolecule's donor atom selectivity and the chemical species of copper present	302:474	Copper is a redox-active antimicrobial metal that can become increasingly toxic depending on the target biomolecule's donor atom selectivity and the chemical species of copper present.
32239052	2	39	theme	redox-active	304:315	arg1	Copper					292:297	Copper	292:297	Copper	292:297	Copper is a redox-active antimicrobial metal that can become increasingly toxic depending on the target biomolecule's donor atom selectivity and the chemical species of copper present.
32239052	3	40	dep	morphology	590:599	arg1	the					586:588	the	586:588	the	586:588	Mass is the traditional measurement of the intrinsic elemental chemistry, but this practice fails to reflect the morphology and surface area reactivity of nanotechnology.
32239052	1	41	theme	promising	145:153	arg1	technology					159:168	a promising new technology	143:168	a promising new technology	143:168	Nanotechnology is a promising new technology, of which antimicrobial metal nanocomposites are predicted to become valuable in medical and food packaging applications.
32239052	1	41	theme	promising	145:153	arg1	Nanotechnology					125:138	Nanotechnology	125:138	Nanotechnology	125:138	Nanotechnology is a promising new technology, of which antimicrobial metal nanocomposites are predicted to become valuable in medical and food packaging applications.
32239052	0	42	theme	S.	37:38	arg1	response					51:58	S. cerevisiae response	37:58	S. cerevisiae response to cellulosic copper nanoparticles	37:93	Metallomic and lipidomic analysis of S. cerevisiae response to cellulosic copper nanoparticles uncovers drivers of toxicity.
32239052	6	43	theme	Further	915:921	arg1	investigation					923:935	Further investigation	915:935	Further investigation into the lipids	915:951	Further investigation into the lipids revealed altered phosphatidylcholine and phosphatidylethanolamine membrane composition, as well as depleted triacylglycerols, suggesting an impact on the Kennedy lipid pathway.
32239052	3	44	theme	area	613:616	arg1	reactivity					618:627	surface area reactivity	605:627	surface area reactivity	605:627	Mass is the traditional measurement of the intrinsic elemental chemistry, but this practice fails to reflect the morphology and surface area reactivity of nanotechnology.
32239052	5	45	theme	Cellular	826:833	arg1	damage					843:848	Cellular surface damage	826:848	Cellular surface damage	826:848	Cellular surface damage was found in scanning electron micrographs upon CMC-Cu exposure.
32239052	7	46	theme	reactive	1145:1152	arg1	species					1161:1167	reactive oxygen species	1145:1167	reactive oxygen species	1145:1167	High levels of reactive oxygen species were measured which likely played a role in the lipid peroxidation detected with CMC-Cu treatment.
32239052	9	47	theme	sensitive	1331:1339	arg1	YJM789					1355:1360	YJM789	1355:1360	YJM789	1355:1360	The copper sensitive yeast strain, YJM789, significantly decreased cellular zinc concentrations while the copper concentrations increased, suggesting a possible ionic mimicry relationship.
32239052	9	47	theme	sensitive	1331:1339	arg1	strain					1347:1352	The copper sensitive yeast strain	1320:1352	The copper sensitive yeast strain	1320:1352	The copper sensitive yeast strain, YJM789, significantly decreased cellular zinc concentrations while the copper concentrations increased, suggesting a possible ionic mimicry relationship.
32239052	4	48	theme	copper	676:681	arg1	CMC-Cu					698:703	CMC-Cu	698:703	CMC-Cu	698:703	The carboxymethyl cellulose copper nanoparticles (CMC-Cu) investigated in this study have unique and undefined toxicity to Saccharomyces cerevisiae that is different from CuSO4.
32239052	4	48	theme	copper	676:681	arg1	nanoparticles					683:695	The carboxymethyl cellulose copper nanoparticles	648:695	The carboxymethyl cellulose copper nanoparticles (CMC-Cu) investigated in this study	648:731	The carboxymethyl cellulose copper nanoparticles (CMC-Cu) investigated in this study have unique and undefined toxicity to Saccharomyces cerevisiae that is different from CuSO4.
32239052	0	49	theme	response	51:58	arg1	analysis					25:32	Metallomic and lipidomic analysis	0:32	Metallomic and lipidomic analysis of S. cerevisiae response to cellulosic copper nanoparticles	0:93	Metallomic and lipidomic analysis of S. cerevisiae response to cellulosic copper nanoparticles uncovers drivers of toxicity.
32239052	9	50	theme	possible	1472:1479	arg1	relationship					1495:1506	a possible ionic mimicry relationship	1470:1506	a possible ionic mimicry relationship	1470:1506	The copper sensitive yeast strain, YJM789, significantly decreased cellular zinc concentrations while the copper concentrations increased, suggesting a possible ionic mimicry relationship.
32239052	7	51	theme	High	1130:1133	arg1	levels					1135:1140	High levels	1130:1140	High levels of reactive oxygen species	1130:1167	High levels of reactive oxygen species were measured which likely played a role in the lipid peroxidation detected with CMC-Cu treatment.
32239052	9	52	theme	yeast	1341:1345	arg1	YJM789					1355:1360	YJM789	1355:1360	YJM789	1355:1360	The copper sensitive yeast strain, YJM789, significantly decreased cellular zinc concentrations while the copper concentrations increased, suggesting a possible ionic mimicry relationship.
32239052	9	52	theme	yeast	1341:1345	arg1	strain					1347:1352	The copper sensitive yeast strain	1320:1352	The copper sensitive yeast strain	1320:1352	The copper sensitive yeast strain, YJM789, significantly decreased cellular zinc concentrations while the copper concentrations increased, suggesting a possible ionic mimicry relationship.
32239052	0	53	dep	S.	37:38	arg1	cerevisiae					40:49	cerevisiae	40:49	cerevisiae	40:49	Metallomic and lipidomic analysis of S. cerevisiae response to cellulosic copper nanoparticles uncovers drivers of toxicity.
32239052	11	54	theme	toxicity	1725:1732	arg1	measurements					1709:1720	forward past traditional measurements	1684:1720	forward past traditional measurements of toxicity	1684:1732	As commonplace objects become more integrated with nanotechnology, humanity must look forward past traditional measurements of toxicity.
32239052	7	55	theme	lipid	1217:1221	arg1	peroxidation					1223:1234	the lipid peroxidation	1213:1234	the lipid peroxidation detected with CMC-Cu treatment	1213:1265	High levels of reactive oxygen species were measured which likely played a role in the lipid peroxidation detected with CMC-Cu treatment.
32239052	9	56	theme	ionic	1481:1485	arg1	relationship					1495:1506	a possible ionic mimicry relationship	1470:1506	a possible ionic mimicry relationship	1470:1506	The copper sensitive yeast strain, YJM789, significantly decreased cellular zinc concentrations while the copper concentrations increased, suggesting a possible ionic mimicry relationship.
32239052	3	57	theme	surface	605:611	arg1	reactivity					618:627	surface area reactivity	605:627	surface area reactivity	605:627	Mass is the traditional measurement of the intrinsic elemental chemistry, but this practice fails to reflect the morphology and surface area reactivity of nanotechnology.
32239052	11	58	theme	traditional	1697:1707	arg1	measurements					1709:1720	forward past traditional measurements	1684:1720	forward past traditional measurements of toxicity	1684:1732	As commonplace objects become more integrated with nanotechnology, humanity must look forward past traditional measurements of toxicity.
32239052	4	59	theme	unique	738:743	arg1	toxicity					759:766	unique and undefined toxicity	738:766	unique and undefined toxicity to Saccharomyces cerevisiae that is different from CuSO4	738:823	The carboxymethyl cellulose copper nanoparticles (CMC-Cu) investigated in this study have unique and undefined toxicity to Saccharomyces cerevisiae that is different from CuSO4.
32239052	6	60	theme	membrane	1019:1026	arg1	composition					1028:1038	phosphatidylethanolamine membrane composition	994:1038	phosphatidylethanolamine membrane composition	994:1038	Further investigation into the lipids revealed altered phosphatidylcholine and phosphatidylethanolamine membrane composition, as well as depleted triacylglycerols, suggesting an impact on the Kennedy lipid pathway.
32239052	3	61	theme	traditional	489:499	arg1	Mass					477:480	Mass	477:480	Mass	477:480	Mass is the traditional measurement of the intrinsic elemental chemistry, but this practice fails to reflect the morphology and surface area reactivity of nanotechnology.
32239052	3	61	theme	traditional	489:499	arg1	measurement					501:511	the traditional measurement	485:511	the traditional measurement of the intrinsic elemental chemistry	485:548	Mass is the traditional measurement of the intrinsic elemental chemistry, but this practice fails to reflect the morphology and surface area reactivity of nanotechnology.
32239052	11	62	theme	commonplace	1601:1611	arg1	objects					1613:1619	commonplace objects	1601:1619	commonplace objects	1601:1619	As commonplace objects become more integrated with nanotechnology, humanity must look forward past traditional measurements of toxicity.
32239052	6	63	theme	lipid	1115:1119	arg1	pathway					1121:1127	the Kennedy lipid pathway	1103:1127	the Kennedy lipid pathway	1103:1127	Further investigation into the lipids revealed altered phosphatidylcholine and phosphatidylethanolamine membrane composition, as well as depleted triacylglycerols, suggesting an impact on the Kennedy lipid pathway.
32239052	9	64	theme	zinc	1396:1399	arg1	concentrations					1401:1414	cellular zinc concentrations	1387:1414	cellular zinc concentrations	1387:1414	The copper sensitive yeast strain, YJM789, significantly decreased cellular zinc concentrations while the copper concentrations increased, suggesting a possible ionic mimicry relationship.
32239052	5	65	theme	scanning	863:870	arg1	micrographs					881:891	scanning electron micrographs	863:891	scanning electron micrographs upon CMC-Cu exposure	863:912	Cellular surface damage was found in scanning electron micrographs upon CMC-Cu exposure.
32239052	4	66	theme	undefined	749:757	arg1	toxicity					759:766	unique and undefined toxicity	738:766	unique and undefined toxicity to Saccharomyces cerevisiae that is different from CuSO4	738:823	The carboxymethyl cellulose copper nanoparticles (CMC-Cu) investigated in this study have unique and undefined toxicity to Saccharomyces cerevisiae that is different from CuSO4.
32239052	6	67	theme	Kennedy	1107:1113	arg1	pathway					1121:1127	the Kennedy lipid pathway	1103:1127	the Kennedy lipid pathway	1103:1127	Further investigation into the lipids revealed altered phosphatidylcholine and phosphatidylethanolamine membrane composition, as well as depleted triacylglycerols, suggesting an impact on the Kennedy lipid pathway.
32239052	0	68	theme	cellulosic	63:72	arg1	nanoparticles					81:93	cellulosic copper nanoparticles	63:93	cellulosic copper nanoparticles	63:93	Metallomic and lipidomic analysis of S. cerevisiae response to cellulosic copper nanoparticles uncovers drivers of toxicity.
32239052	5	69	theme	CMC-Cu	898:903	arg1	exposure					905:912	CMC-Cu exposure	898:912	CMC-Cu exposure	898:912	Cellular surface damage was found in scanning electron micrographs upon CMC-Cu exposure.
32239052	4	70	theme	cellulose	666:674	arg1	CMC-Cu					698:703	CMC-Cu	698:703	CMC-Cu	698:703	The carboxymethyl cellulose copper nanoparticles (CMC-Cu) investigated in this study have unique and undefined toxicity to Saccharomyces cerevisiae that is different from CuSO4.
32239052	4	70	theme	cellulose	666:674	arg1	nanoparticles					683:695	The carboxymethyl cellulose copper nanoparticles	648:695	The carboxymethyl cellulose copper nanoparticles (CMC-Cu) investigated in this study	648:731	The carboxymethyl cellulose copper nanoparticles (CMC-Cu) investigated in this study have unique and undefined toxicity to Saccharomyces cerevisiae that is different from CuSO4.
32239052	2	71	theme	target	389:394	arg1	biomolecule					396:406	the target biomolecule's	385:408	the target biomolecule's donor atom selectivity	385:431	Copper is a redox-active antimicrobial metal that can become increasingly toxic depending on the target biomolecule's donor atom selectivity and the chemical species of copper present.
32239052	5	72	theme	surface	835:841	arg1	damage					843:848	Cellular surface damage	826:848	Cellular surface damage	826:848	Cellular surface damage was found in scanning electron micrographs upon CMC-Cu exposure.
32239052	6	73	theme	phosphatidylethanolamine	994:1017	arg1	composition					1028:1038	phosphatidylethanolamine membrane composition	994:1038	phosphatidylethanolamine membrane composition	994:1038	Further investigation into the lipids revealed altered phosphatidylcholine and phosphatidylethanolamine membrane composition, as well as depleted triacylglycerols, suggesting an impact on the Kennedy lipid pathway.
32239052	3	74	theme	nanotechnology	632:645	arg1	reactivity					618:627	surface area reactivity	605:627	surface area reactivity	605:627	Mass is the traditional measurement of the intrinsic elemental chemistry, but this practice fails to reflect the morphology and surface area reactivity of nanotechnology.
32239052	3	74	theme	nanotechnology	632:645	arg1	morphology					590:599	morphology	590:599	morphology	590:599	Mass is the traditional measurement of the intrinsic elemental chemistry, but this practice fails to reflect the morphology and surface area reactivity of nanotechnology.
32239052	1	75	theme	new	155:157	arg1	technology					159:168	a promising new technology	143:168	a promising new technology	143:168	Nanotechnology is a promising new technology, of which antimicrobial metal nanocomposites are predicted to become valuable in medical and food packaging applications.
32239052	1	75	theme	new	155:157	arg1	Nanotechnology					125:138	Nanotechnology	125:138	Nanotechnology	125:138	Nanotechnology is a promising new technology, of which antimicrobial metal nanocomposites are predicted to become valuable in medical and food packaging applications.
32239052	1	76	theme	medical	251:257	arg1	applications					278:289	medical and food packaging applications	251:289	medical and food packaging applications	251:289	Nanotechnology is a promising new technology, of which antimicrobial metal nanocomposites are predicted to become valuable in medical and food packaging applications.
32168892	0	0	theme	Valvular	74:81	arg1	Calcification					101:113	Valvular Interstitial Cell Calcification	74:113	Valvular Interstitial Cell Calcification	74:113	Proteoglycan 4 is Increased in Human Calcified Aortic Valves and Enhances Valvular Interstitial Cell Calcification.
32168892	5	1	theme	valve	730:734	arg1	replacement					736:746	aortic valve replacement	723:746	aortic valve replacement	723:746	Here, transcriptomics, histology, and immunohistochemistry were performed in human aortic valves from patients undergoing aortic valve replacement.
32168892	8	2	theme	PRG4	1002:1005	arg1	levels					992:997	mRNA levels	987:997	mRNA levels of PRG4	987:1005	In addition, mRNA levels of PRG4 positively associated with mRNA for proteins involved in cardiovascular calcification.
32168892	3	3	theme	aortic	478:483	arg1	valves					485:490	aortic valves	478:490	aortic valves	478:490	Particularly, proteoglycan 4 (PRG4) has been identified in fibrotic parts of aortic valves.
32168892	4	4	theme	AVS	537:539	arg1	context					526:532	the context	522:532	the context of AVS and aortic valve calcification	522:570	However, the role of PRG4 in the context of AVS and aortic valve calcification has not yet been determined.
32168892	10	5	theme	AVS	1347:1349	arg1	development					1332:1342	the development	1328:1342	the development of AVS	1328:1349	In summary, PRG4 was upregulated in the development of AVS and promoted VIC osteogenic differentiation and calcification.
32168892	3	6	theme	valves	485:490	arg1	parts					469:473	fibrotic parts	460:473	fibrotic parts of aortic valves	460:490	Particularly, proteoglycan 4 (PRG4) has been identified in fibrotic parts of aortic valves.
32168892	1	7	theme	progressive	236:246	arg1	narrowing					248:256	progressive narrowing	236:256	progressive narrowing of the aortic valve	236:276	Aortic valve stenosis (AVS), a consequence of increased fibrosis and calcification of the aortic valve leaflets, causes progressive narrowing of the aortic valve.
32168892	0	8	theme	Cell	96:99	arg1	Calcification					101:113	Valvular Interstitial Cell Calcification	74:113	Valvular Interstitial Cell Calcification	74:113	Proteoglycan 4 is Increased in Human Calcified Aortic Valves and Enhances Valvular Interstitial Cell Calcification.
32168892	9	9	theme	bone	1218:1221	arg1	protein					1237:1243	bone morphogenetic protein 2	1218:1245	bone morphogenetic protein 2	1218:1245	Treatment of VICs with recombinant human PRG4 enhanced phosphate-induced calcification and increased the mRNA expression of bone morphogenetic protein 2 and the runt-related transcription factor 2.
32168892	9	10	theme	mRNA	1199:1202	arg1	expression					1204:1213	the mRNA expression	1195:1213	the mRNA expression of bone morphogenetic protein 2 and the runt-related transcription factor 2	1195:1289	Treatment of VICs with recombinant human PRG4 enhanced phosphate-induced calcification and increased the mRNA expression of bone morphogenetic protein 2 and the runt-related transcription factor 2.
32168892	11	11	theme	AVS	1529:1531	arg1	progression					1514:1524	the progression	1510:1524	the progression of AVS	1510:1531	These results suggest that an altered valve leaflet proteoglycan composition may play a role in the progression of AVS.
32168892	4	12	theme	aortic	545:550	arg1	calcification					558:570	aortic valve calcification	545:570	aortic valve calcification	545:570	However, the role of PRG4 in the context of AVS and aortic valve calcification has not yet been determined.
32168892	0	13	theme	Interstitial	83:94	arg1	Calcification					101:113	Valvular Interstitial Cell Calcification	74:113	Valvular Interstitial Cell Calcification	74:113	Proteoglycan 4 is Increased in Human Calcified Aortic Valves and Enhances Valvular Interstitial Cell Calcification.
32168892	9	14	theme	recombinant	1117:1127	arg1	PRG4					1135:1138	recombinant human PRG4	1117:1138	recombinant human PRG4	1117:1138	Treatment of VICs with recombinant human PRG4 enhanced phosphate-induced calcification and increased the mRNA expression of bone morphogenetic protein 2 and the runt-related transcription factor 2.
32168892	7	15	theme	aortic	929:934	arg1	valves					936:941	aortic valves	929:941	aortic valves	929:941	PRG4 was significantly upregulated in thickened and calcified regions of aortic valves compared with healthy regions.
32168892	11	16	theme	leaflet	1458:1464	arg1	composition					1479:1489	an altered valve leaflet proteoglycan composition	1441:1489	an altered valve leaflet proteoglycan composition	1441:1489	These results suggest that an altered valve leaflet proteoglycan composition may play a role in the progression of AVS.
32168892	2	17	theme	valve	330:334	arg1	Proteoglycans					279:291	Proteoglycans	279:291	Proteoglycans	279:291	Proteoglycans, structural components of the aortic valve, accumulate in regions with fibrosis and moderate calcification.
32168892	2	17	theme	valve	330:334	arg1	components					305:314	structural components	294:314	structural components of the aortic valve	294:334	Proteoglycans, structural components of the aortic valve, accumulate in regions with fibrosis and moderate calcification.
32168892	7	18	theme	valves	936:941	arg1	regions					918:924	thickened and calcified regions	894:924	thickened and calcified regions of aortic valves	894:941	PRG4 was significantly upregulated in thickened and calcified regions of aortic valves compared with healthy regions.
32168892	10	19	theme	VIC	1364:1366	arg1	differentiation					1379:1393	VIC osteogenic differentiation	1364:1393	VIC osteogenic differentiation	1364:1393	In summary, PRG4 was upregulated in the development of AVS and promoted VIC osteogenic differentiation and calcification.
32168892	2	20	theme	moderate	377:384	arg1	calcification					386:398	moderate calcification	377:398	moderate calcification	377:398	Proteoglycans, structural components of the aortic valve, accumulate in regions with fibrosis and moderate calcification.
32168892	6	21	theme	interstitial	761:772	arg1	VICs					781:784	VICs	781:784	VICs	781:784	Human valve interstitial cells (VICs) were used for calcification experiments and RNA expression analysis.
32168892	6	21	theme	interstitial	761:772	arg1	cells					774:778	Human valve interstitial cells	749:778	Human valve interstitial cells (VICs)	749:785	Human valve interstitial cells (VICs) were used for calcification experiments and RNA expression analysis.
32168892	4	22	theme	valve	552:556	arg1	calcification					558:570	aortic valve calcification	545:570	aortic valve calcification	545:570	However, the role of PRG4 in the context of AVS and aortic valve calcification has not yet been determined.
32168892	11	23	theme	altered	1444:1450	arg1	composition					1479:1489	an altered valve leaflet proteoglycan composition	1441:1489	an altered valve leaflet proteoglycan composition	1441:1489	These results suggest that an altered valve leaflet proteoglycan composition may play a role in the progression of AVS.
32168892	11	24	theme	proteoglycan	1466:1477	arg1	composition					1479:1489	an altered valve leaflet proteoglycan composition	1441:1489	an altered valve leaflet proteoglycan composition	1441:1489	These results suggest that an altered valve leaflet proteoglycan composition may play a role in the progression of AVS.
32168892	6	25	theme	valve	755:759	arg1	VICs					781:784	VICs	781:784	VICs	781:784	Human valve interstitial cells (VICs) were used for calcification experiments and RNA expression analysis.
32168892	6	25	theme	valve	755:759	arg1	cells					774:778	Human valve interstitial cells	749:778	Human valve interstitial cells (VICs)	749:785	Human valve interstitial cells (VICs) were used for calcification experiments and RNA expression analysis.
32168892	11	26	theme	valve	1452:1456	arg1	composition					1479:1489	an altered valve leaflet proteoglycan composition	1441:1489	an altered valve leaflet proteoglycan composition	1441:1489	These results suggest that an altered valve leaflet proteoglycan composition may play a role in the progression of AVS.
32168892	1	27	theme	increased	162:170	arg1	fibrosis					172:179	increased fibrosis	162:179	increased fibrosis	162:179	Aortic valve stenosis (AVS), a consequence of increased fibrosis and calcification of the aortic valve leaflets, causes progressive narrowing of the aortic valve.
32168892	6	28	used	used	792:795	arg2	VICs					781:784	VICs	781:784	VICs	781:784	Human valve interstitial cells (VICs) were used for calcification experiments and RNA expression analysis.
32168892	6	28	used	used	792:795	arg2	cells					774:778	Human valve interstitial cells	749:778	Human valve interstitial cells (VICs)	749:785	Human valve interstitial cells (VICs) were used for calcification experiments and RNA expression analysis.
32168892	6	29	theme	Human	749:753	arg1	VICs					781:784	VICs	781:784	VICs	781:784	Human valve interstitial cells (VICs) were used for calcification experiments and RNA expression analysis.
32168892	6	29	theme	Human	749:753	arg1	cells					774:778	Human valve interstitial cells	749:778	Human valve interstitial cells (VICs)	749:785	Human valve interstitial cells (VICs) were used for calcification experiments and RNA expression analysis.
32168892	2	30	theme	structural	294:303	arg1	Proteoglycans					279:291	Proteoglycans	279:291	Proteoglycans	279:291	Proteoglycans, structural components of the aortic valve, accumulate in regions with fibrosis and moderate calcification.
32168892	2	30	theme	structural	294:303	arg1	components					305:314	structural components	294:314	structural components of the aortic valve	294:334	Proteoglycans, structural components of the aortic valve, accumulate in regions with fibrosis and moderate calcification.
32168892	1	31	theme	fibrosis	172:179	arg1	stenosis					129:136	Aortic valve stenosis	116:136	Aortic valve stenosis (AVS)	116:142	Aortic valve stenosis (AVS), a consequence of increased fibrosis and calcification of the aortic valve leaflets, causes progressive narrowing of the aortic valve.
32168892	1	31	theme	fibrosis	172:179	arg1	consequence					147:157	a consequence	145:157	a consequence of increased fibrosis and calcification of the aortic valve leaflets	145:226	Aortic valve stenosis (AVS), a consequence of increased fibrosis and calcification of the aortic valve leaflets, causes progressive narrowing of the aortic valve.
32168892	1	32	theme	aortic	265:270	arg1	valve					272:276	the aortic valve	261:276	the aortic valve	261:276	Aortic valve stenosis (AVS), a consequence of increased fibrosis and calcification of the aortic valve leaflets, causes progressive narrowing of the aortic valve.
32168892	7	33	theme	calcified	908:916	arg1	regions					918:924	thickened and calcified regions	894:924	thickened and calcified regions of aortic valves	894:941	PRG4 was significantly upregulated in thickened and calcified regions of aortic valves compared with healthy regions.
32168892	9	34	theme	morphogenetic	1223:1235	arg1	protein					1237:1243	bone morphogenetic protein 2	1218:1245	bone morphogenetic protein 2	1218:1245	Treatment of VICs with recombinant human PRG4 enhanced phosphate-induced calcification and increased the mRNA expression of bone morphogenetic protein 2 and the runt-related transcription factor 2.
32168892	1	35	theme	valve	272:276	arg1	narrowing					248:256	progressive narrowing	236:256	progressive narrowing of the aortic valve	236:276	Aortic valve stenosis (AVS), a consequence of increased fibrosis and calcification of the aortic valve leaflets, causes progressive narrowing of the aortic valve.
32168892	6	36	theme	expression	835:844	arg1	analysis					846:853	RNA expression analysis	831:853	RNA expression analysis	831:853	Human valve interstitial cells (VICs) were used for calcification experiments and RNA expression analysis.
32168892	1	37	theme	calcification	185:197	arg1	stenosis					129:136	Aortic valve stenosis	116:136	Aortic valve stenosis (AVS)	116:142	Aortic valve stenosis (AVS), a consequence of increased fibrosis and calcification of the aortic valve leaflets, causes progressive narrowing of the aortic valve.
32168892	1	37	theme	calcification	185:197	arg1	consequence					147:157	a consequence	145:157	a consequence of increased fibrosis and calcification of the aortic valve leaflets	145:226	Aortic valve stenosis (AVS), a consequence of increased fibrosis and calcification of the aortic valve leaflets, causes progressive narrowing of the aortic valve.
32168892	0	38	theme	Human	31:35	arg1	Valves					54:59	Human Calcified Aortic Valves	31:59	Human Calcified Aortic Valves	31:59	Proteoglycan 4 is Increased in Human Calcified Aortic Valves and Enhances Valvular Interstitial Cell Calcification.
32168892	2	39	theme	aortic	323:328	arg1	valve					330:334	the aortic valve	319:334	the aortic valve	319:334	Proteoglycans, structural components of the aortic valve, accumulate in regions with fibrosis and moderate calcification.
32168892	5	40	from	patients	703:710	arg1	valves					691:696	human aortic valves	678:696	human aortic valves from patients undergoing aortic valve replacement	678:746	Here, transcriptomics, histology, and immunohistochemistry were performed in human aortic valves from patients undergoing aortic valve replacement.
32168892	9	41	theme	VICs	1107:1110	arg1	Treatment					1094:1102	Treatment	1094:1102	Treatment of VICs with recombinant human PRG4	1094:1138	Treatment of VICs with recombinant human PRG4 enhanced phosphate-induced calcification and increased the mRNA expression of bone morphogenetic protein 2 and the runt-related transcription factor 2.
32168892	4	42	from	role	506:509	arg1	context					526:532	the context	522:532	the context of AVS and aortic valve calcification	522:570	However, the role of PRG4 in the context of AVS and aortic valve calcification has not yet been determined.
32168892	5	43	theme	human	678:682	arg1	valves					691:696	human aortic valves	678:696	human aortic valves from patients undergoing aortic valve replacement	678:746	Here, transcriptomics, histology, and immunohistochemistry were performed in human aortic valves from patients undergoing aortic valve replacement.
32168892	0	44	theme	Aortic	47:52	arg1	Valves					54:59	Human Calcified Aortic Valves	31:59	Human Calcified Aortic Valves	31:59	Proteoglycan 4 is Increased in Human Calcified Aortic Valves and Enhances Valvular Interstitial Cell Calcification.
32168892	5	45	theme	aortic	684:689	arg1	valves					691:696	human aortic valves	678:696	human aortic valves from patients undergoing aortic valve replacement	678:746	Here, transcriptomics, histology, and immunohistochemistry were performed in human aortic valves from patients undergoing aortic valve replacement.
32168892	4	46	theme	PRG4	514:517	arg1	role					506:509	the role	502:509	the role of PRG4 in the context of AVS and aortic valve calcification	502:570	However, the role of PRG4 in the context of AVS and aortic valve calcification has not yet been determined.
32168892	9	47	theme	protein	1237:1243	arg1	expression					1204:1213	the mRNA expression	1195:1213	the mRNA expression of bone morphogenetic protein 2 and the runt-related transcription factor 2	1195:1289	Treatment of VICs with recombinant human PRG4 enhanced phosphate-induced calcification and increased the mRNA expression of bone morphogenetic protein 2 and the runt-related transcription factor 2.
32168892	1	48	theme	Aortic	116:121	arg1	stenosis					129:136	Aortic valve stenosis	116:136	Aortic valve stenosis (AVS)	116:142	Aortic valve stenosis (AVS), a consequence of increased fibrosis and calcification of the aortic valve leaflets, causes progressive narrowing of the aortic valve.
32168892	1	48	theme	Aortic	116:121	arg1	consequence					147:157	a consequence	145:157	a consequence of increased fibrosis and calcification of the aortic valve leaflets	145:226	Aortic valve stenosis (AVS), a consequence of increased fibrosis and calcification of the aortic valve leaflets, causes progressive narrowing of the aortic valve.
32168892	1	48	theme	Aortic	116:121	arg1	AVS					139:141	AVS	139:141	AVS	139:141	Aortic valve stenosis (AVS), a consequence of increased fibrosis and calcification of the aortic valve leaflets, causes progressive narrowing of the aortic valve.
32168892	0	49	theme	Calcified	37:45	arg1	Valves					54:59	Human Calcified Aortic Valves	31:59	Human Calcified Aortic Valves	31:59	Proteoglycan 4 is Increased in Human Calcified Aortic Valves and Enhances Valvular Interstitial Cell Calcification.
32168892	9	50	theme	transcription	1268:1280	arg1	factor					1282:1287	the runt-related transcription factor 2	1251:1289	the runt-related transcription factor 2	1251:1289	Treatment of VICs with recombinant human PRG4 enhanced phosphate-induced calcification and increased the mRNA expression of bone morphogenetic protein 2 and the runt-related transcription factor 2.
32168892	6	51	theme	calcification	801:813	arg1	experiments					815:825	calcification experiments	801:825	calcification experiments	801:825	Human valve interstitial cells (VICs) were used for calcification experiments and RNA expression analysis.
32168892	9	52	theme	runt-related	1255:1266	arg1	factor					1282:1287	the runt-related transcription factor 2	1251:1289	the runt-related transcription factor 2	1251:1289	Treatment of VICs with recombinant human PRG4 enhanced phosphate-induced calcification and increased the mRNA expression of bone morphogenetic protein 2 and the runt-related transcription factor 2.
32168892	9	53	theme	human	1129:1133	arg1	PRG4					1135:1138	recombinant human PRG4	1117:1138	recombinant human PRG4	1117:1138	Treatment of VICs with recombinant human PRG4 enhanced phosphate-induced calcification and increased the mRNA expression of bone morphogenetic protein 2 and the runt-related transcription factor 2.
32168892	1	54	theme	valve	123:127	arg1	stenosis					129:136	Aortic valve stenosis	116:136	Aortic valve stenosis (AVS)	116:142	Aortic valve stenosis (AVS), a consequence of increased fibrosis and calcification of the aortic valve leaflets, causes progressive narrowing of the aortic valve.
32168892	1	54	theme	valve	123:127	arg1	consequence					147:157	a consequence	145:157	a consequence of increased fibrosis and calcification of the aortic valve leaflets	145:226	Aortic valve stenosis (AVS), a consequence of increased fibrosis and calcification of the aortic valve leaflets, causes progressive narrowing of the aortic valve.
32168892	1	54	theme	valve	123:127	arg1	AVS					139:141	AVS	139:141	AVS	139:141	Aortic valve stenosis (AVS), a consequence of increased fibrosis and calcification of the aortic valve leaflets, causes progressive narrowing of the aortic valve.
32168892	8	55	theme	mRNA	987:990	arg1	levels					992:997	mRNA levels	987:997	mRNA levels of PRG4	987:1005	In addition, mRNA levels of PRG4 positively associated with mRNA for proteins involved in cardiovascular calcification.
32168892	9	56	theme	factor	1282:1287	arg1	expression					1204:1213	the mRNA expression	1195:1213	the mRNA expression of bone morphogenetic protein 2 and the runt-related transcription factor 2	1195:1289	Treatment of VICs with recombinant human PRG4 enhanced phosphate-induced calcification and increased the mRNA expression of bone morphogenetic protein 2 and the runt-related transcription factor 2.
32168892	9	57	with	Treatment	1094:1102	arg1	PRG4					1135:1138	recombinant human PRG4	1117:1138	recombinant human PRG4	1117:1138	Treatment of VICs with recombinant human PRG4 enhanced phosphate-induced calcification and increased the mRNA expression of bone morphogenetic protein 2 and the runt-related transcription factor 2.
32168892	7	58	theme	thickened	894:902	arg1	regions					918:924	thickened and calcified regions	894:924	thickened and calcified regions of aortic valves	894:941	PRG4 was significantly upregulated in thickened and calcified regions of aortic valves compared with healthy regions.
32168892	1	59	theme	aortic	206:211	arg1	leaflets					219:226	the aortic valve leaflets	202:226	the aortic valve leaflets	202:226	Aortic valve stenosis (AVS), a consequence of increased fibrosis and calcification of the aortic valve leaflets, causes progressive narrowing of the aortic valve.
32168892	2	60	with	regions	351:357	arg1	fibrosis					364:371	fibrosis	364:371	fibrosis	364:371	Proteoglycans, structural components of the aortic valve, accumulate in regions with fibrosis and moderate calcification.
32168892	2	60	with	regions	351:357	arg1	calcification					386:398	moderate calcification	377:398	moderate calcification	377:398	Proteoglycans, structural components of the aortic valve, accumulate in regions with fibrosis and moderate calcification.
32168892	3	61	theme	fibrotic	460:467	arg1	parts					469:473	fibrotic parts	460:473	fibrotic parts of aortic valves	460:490	Particularly, proteoglycan 4 (PRG4) has been identified in fibrotic parts of aortic valves.
32168892	1	62	theme	valve	213:217	arg1	leaflets					219:226	the aortic valve leaflets	202:226	the aortic valve leaflets	202:226	Aortic valve stenosis (AVS), a consequence of increased fibrosis and calcification of the aortic valve leaflets, causes progressive narrowing of the aortic valve.
32168892	6	63	theme	RNA	831:833	arg1	analysis					846:853	RNA expression analysis	831:853	RNA expression analysis	831:853	Human valve interstitial cells (VICs) were used for calcification experiments and RNA expression analysis.
32168892	10	64	theme	osteogenic	1368:1377	arg1	differentiation					1379:1393	VIC osteogenic differentiation	1364:1393	VIC osteogenic differentiation	1364:1393	In summary, PRG4 was upregulated in the development of AVS and promoted VIC osteogenic differentiation and calcification.
32168892	9	65	theme	phosphate-induced	1149:1165	arg1	calcification					1167:1179	phosphate-induced calcification	1149:1179	phosphate-induced calcification	1149:1179	Treatment of VICs with recombinant human PRG4 enhanced phosphate-induced calcification and increased the mRNA expression of bone morphogenetic protein 2 and the runt-related transcription factor 2.
32168892	1	66	theme	leaflets	219:226	arg1	calcification					185:197	calcification	185:197	calcification of the aortic valve leaflets	185:226	Aortic valve stenosis (AVS), a consequence of increased fibrosis and calcification of the aortic valve leaflets, causes progressive narrowing of the aortic valve.
32168892	1	66	theme	leaflets	219:226	arg1	fibrosis					172:179	increased fibrosis	162:179	increased fibrosis	162:179	Aortic valve stenosis (AVS), a consequence of increased fibrosis and calcification of the aortic valve leaflets, causes progressive narrowing of the aortic valve.
32168892	5	67	theme	aortic	723:728	arg1	replacement					736:746	aortic valve replacement	723:746	aortic valve replacement	723:746	Here, transcriptomics, histology, and immunohistochemistry were performed in human aortic valves from patients undergoing aortic valve replacement.
32168892	7	68	theme	healthy	957:963	arg1	regions					965:971	healthy regions	957:971	healthy regions	957:971	PRG4 was significantly upregulated in thickened and calcified regions of aortic valves compared with healthy regions.
32168892	4	69	theme	calcification	558:570	arg1	context					526:532	the context	522:532	the context of AVS and aortic valve calcification	522:570	However, the role of PRG4 in the context of AVS and aortic valve calcification has not yet been determined.
32168892	8	70	theme	cardiovascular	1064:1077	arg1	calcification					1079:1091	cardiovascular calcification	1064:1091	cardiovascular calcification	1064:1091	In addition, mRNA levels of PRG4 positively associated with mRNA for proteins involved in cardiovascular calcification.
32806778	6	0	from	maintenance	1101:1111	arg1	culture					1147:1153	culture	1147:1153	culture	1147:1153	The maintenance of differentiated chondrocytes in culture was then evaluated via morphological analysis of cell phenotype, GAG production and cytoskeleton organization.
32806778	6	1	theme	GAG	1220:1222	arg1	production					1224:1233	GAG production	1220:1233	GAG production	1220:1233	The maintenance of differentiated chondrocytes in culture was then evaluated via morphological analysis of cell phenotype, GAG production and cytoskeleton organization.
32806778	3	2	theme	native	562:567	arg1	collagen					569:576	native collagen	562:576	native collagen	562:576	For this purpose, we produced an injectable marine collagen-based hydrogel, by mixing native collagen from the jellyfish Rhizostoma pulmo with hydroxy-phenyl-propionic acid (HPA)-functionalized marine gelatin.
32806778	4	3	theme	cytotoxic	881:889	arg1	effects					891:897	cytotoxic effects	881:897	cytotoxic effects	881:897	This biocompatible hydrogel formulation, due to the ability of enzymatically reticulate using horseradish peroxidase (HPR) and H2O2, gives the possibility of trap cells inside, in the absence of cytotoxic effects, during the cross-linking process.
32806778	6	4	theme	phenotype	1209:1217	arg1	analysis					1192:1199	morphological analysis	1178:1199	morphological analysis of cell phenotype, GAG production and cytoskeleton organization	1178:1263	The maintenance of differentiated chondrocytes in culture was then evaluated via morphological analysis of cell phenotype, GAG production and cytoskeleton organization.
32806778	4	5	theme	due	727:729	arg1	formulation					714:724	This biocompatible hydrogel formulation	686:724	This biocompatible hydrogel formulation	686:724	This biocompatible hydrogel formulation, due to the ability of enzymatically reticulate using horseradish peroxidase (HPR) and H2O2, gives the possibility of trap cells inside, in the absence of cytotoxic effects, during the cross-linking process.
32806778	2	6	theme	differentiated	338:351	arg1	state					353:357	the differentiated state	334:357	the differentiated state	334:357	The use of autologous chondrocyte transplants represent an effective strategy for cell regeneration; however, preserving the differentiated state, which ensures the ability to regenerate damaged cartilage, represents the main challenge during in vitro culturing.
32806778	2	7	theme	chondrocyte	235:245	arg1	transplants					247:257	autologous chondrocyte transplants	224:257	autologous chondrocyte transplants	224:257	The use of autologous chondrocyte transplants represent an effective strategy for cell regeneration; however, preserving the differentiated state, which ensures the ability to regenerate damaged cartilage, represents the main challenge during in vitro culturing.
32806778	8	8	theme	biochemical	1456:1466	arg1	properties					1468:1477	the biochemical properties	1452:1477	the biochemical properties of marine collagen	1452:1496	This, combined with the biochemical properties of marine collagen, represents a promising strategy for maintaining in vitro the cellular phenotype in the aim of the use of autologous chondrocytes in regenerative medicine practices.
32806778	6	9	theme	chondrocytes	1131:1142	arg1	maintenance					1101:1111	The maintenance	1097:1111	The maintenance of differentiated chondrocytes in culture	1097:1153	The maintenance of differentiated chondrocytes in culture was then evaluated via morphological analysis of cell phenotype, GAG production and cytoskeleton organization.
32806778	5	10	from	changes	1045:1051	arg1	concentration					1060:1072	the concentration	1056:1072	the concentration of polymer precursors	1056:1094	Moreover, it enables the modulation of the hydrogel stiffness merely varying the concentration of H2O2 without changes in the concentration of polymer precursors.
32806778	4	11	theme	biocompatible	691:703	arg1	formulation					714:724	This biocompatible hydrogel formulation	686:724	This biocompatible hydrogel formulation	686:724	This biocompatible hydrogel formulation, due to the ability of enzymatically reticulate using horseradish peroxidase (HPR) and H2O2, gives the possibility of trap cells inside, in the absence of cytotoxic effects, during the cross-linking process.
32806778	0	12	from	Maintenance	64:74	arg1	Applications					129:140	Tissue Engineering Applications	110:140	Tissue Engineering Applications	110:140	Production of Injectable Marine Collagen-Based Hydrogel for the Maintenance of Differentiated Chondrocytes in Tissue Engineering Applications.
32806778	4	13	theme	reticulate	763:772	arg1	HPR					804:806	HPR	804:806	HPR	804:806	This biocompatible hydrogel formulation, due to the ability of enzymatically reticulate using horseradish peroxidase (HPR) and H2O2, gives the possibility of trap cells inside, in the absence of cytotoxic effects, during the cross-linking process.
32806778	4	13	theme	reticulate	763:772	arg1	peroxidase					792:801	using horseradish peroxidase	774:801	using horseradish peroxidase (HPR)	774:807	This biocompatible hydrogel formulation, due to the ability of enzymatically reticulate using horseradish peroxidase (HPR) and H2O2, gives the possibility of trap cells inside, in the absence of cytotoxic effects, during the cross-linking process.
32806778	3	14	theme	hydroxy-phenyl-propionic	619:642	arg1	gelatin					677:683	hydroxy-phenyl-propionic acid (HPA)-functionalized marine gelatin	619:683	hydroxy-phenyl-propionic acid (HPA)-functionalized marine gelatin	619:683	For this purpose, we produced an injectable marine collagen-based hydrogel, by mixing native collagen from the jellyfish Rhizostoma pulmo with hydroxy-phenyl-propionic acid (HPA)-functionalized marine gelatin.
32806778	2	15	theme	cell	295:298	arg1	regeneration					300:311	cell regeneration	295:311	cell regeneration	295:311	The use of autologous chondrocyte transplants represent an effective strategy for cell regeneration; however, preserving the differentiated state, which ensures the ability to regenerate damaged cartilage, represents the main challenge during in vitro culturing.
32806778	4	16	theme	horseradish	780:790	arg1	HPR					804:806	HPR	804:806	HPR	804:806	This biocompatible hydrogel formulation, due to the ability of enzymatically reticulate using horseradish peroxidase (HPR) and H2O2, gives the possibility of trap cells inside, in the absence of cytotoxic effects, during the cross-linking process.
32806778	4	16	theme	horseradish	780:790	arg1	peroxidase					792:801	using horseradish peroxidase	774:801	using horseradish peroxidase (HPR)	774:807	This biocompatible hydrogel formulation, due to the ability of enzymatically reticulate using horseradish peroxidase (HPR) and H2O2, gives the possibility of trap cells inside, in the absence of cytotoxic effects, during the cross-linking process.
32806778	5	17	theme	precursors	1085:1094	arg1	concentration					1060:1072	the concentration	1056:1072	the concentration of polymer precursors	1056:1094	Moreover, it enables the modulation of the hydrogel stiffness merely varying the concentration of H2O2 without changes in the concentration of polymer precursors.
32806778	3	18	theme	marine	670:675	arg1	gelatin					677:683	hydroxy-phenyl-propionic acid (HPA)-functionalized marine gelatin	619:683	hydroxy-phenyl-propionic acid (HPA)-functionalized marine gelatin	619:683	For this purpose, we produced an injectable marine collagen-based hydrogel, by mixing native collagen from the jellyfish Rhizostoma pulmo with hydroxy-phenyl-propionic acid (HPA)-functionalized marine gelatin.
32806778	6	19	theme	organization	1252:1263	arg1	analysis					1192:1199	morphological analysis	1178:1199	morphological analysis of cell phenotype, GAG production and cytoskeleton organization	1178:1263	The maintenance of differentiated chondrocytes in culture was then evaluated via morphological analysis of cell phenotype, GAG production and cytoskeleton organization.
32806778	2	20	theme	effective	272:280	arg1	strategy					282:289	an effective strategy	269:289	an effective strategy for cell regeneration	269:311	The use of autologous chondrocyte transplants represent an effective strategy for cell regeneration; however, preserving the differentiated state, which ensures the ability to regenerate damaged cartilage, represents the main challenge during in vitro culturing.
32806778	0	21	theme	Differentiated	79:92	arg1	Chondrocytes					94:105	Differentiated Chondrocytes	79:105	Differentiated Chondrocytes in Tissue Engineering Applications	79:140	Production of Injectable Marine Collagen-Based Hydrogel for the Maintenance of Differentiated Chondrocytes in Tissue Engineering Applications.
32806778	1	22	theme	limited	181:187	arg1	ability					189:195	limited ability	181:195	limited ability of self-repair	181:210	Cartilage is an avascular tissue with limited ability of self-repair.
32806778	8	23	theme	use	1597:1599	arg1	aim					1586:1588	the aim	1582:1588	the aim of the use of autologous chondrocytes in regenerative medicine practices	1582:1661	This, combined with the biochemical properties of marine collagen, represents a promising strategy for maintaining in vitro the cellular phenotype in the aim of the use of autologous chondrocytes in regenerative medicine practices.
32806778	4	24	theme	H2O2	813:816	arg1	ability					738:744	the ability	734:744	the ability of enzymatically reticulate using horseradish peroxidase (HPR) and H2O2	734:816	This biocompatible hydrogel formulation, due to the ability of enzymatically reticulate using horseradish peroxidase (HPR) and H2O2, gives the possibility of trap cells inside, in the absence of cytotoxic effects, during the cross-linking process.
32806778	1	25	with	tissue	169:174	arg1	ability					189:195	limited ability	181:195	limited ability of self-repair	181:210	Cartilage is an avascular tissue with limited ability of self-repair.
32806778	8	26	theme	autologous	1604:1613	arg1	chondrocytes					1615:1626	autologous chondrocytes	1604:1626	autologous chondrocytes	1604:1626	This, combined with the biochemical properties of marine collagen, represents a promising strategy for maintaining in vitro the cellular phenotype in the aim of the use of autologous chondrocytes in regenerative medicine practices.
32806778	0	27	theme	Engineering	117:127	arg1	Applications					129:140	Tissue Engineering Applications	110:140	Tissue Engineering Applications	110:140	Production of Injectable Marine Collagen-Based Hydrogel for the Maintenance of Differentiated Chondrocytes in Tissue Engineering Applications.
32806778	8	28	from	use	1597:1599	arg1	practices					1653:1661	regenerative medicine practices	1631:1661	regenerative medicine practices	1631:1661	This, combined with the biochemical properties of marine collagen, represents a promising strategy for maintaining in vitro the cellular phenotype in the aim of the use of autologous chondrocytes in regenerative medicine practices.
32806778	7	29	theme	program	1423:1429	arg1	promotion					1377:1385	the promotion	1373:1385	the promotion of the chondrogenic gene expression program	1373:1429	Additionally, gene expression profiling of differentiation/dedifferentiation markers provided evidence for the promotion of the chondrogenic gene expression program.
32806778	8	30	theme	medicine	1644:1651	arg1	practices					1653:1661	regenerative medicine practices	1631:1661	regenerative medicine practices	1631:1661	This, combined with the biochemical properties of marine collagen, represents a promising strategy for maintaining in vitro the cellular phenotype in the aim of the use of autologous chondrocytes in regenerative medicine practices.
32806778	8	31	theme	marine	1482:1487	arg1	collagen					1489:1496	marine collagen	1482:1496	marine collagen	1482:1496	This, combined with the biochemical properties of marine collagen, represents a promising strategy for maintaining in vitro the cellular phenotype in the aim of the use of autologous chondrocytes in regenerative medicine practices.
32806778	2	32	theme	in	456:457	arg1	culturing					465:473	in vitro culturing	456:473	in vitro culturing	456:473	The use of autologous chondrocyte transplants represent an effective strategy for cell regeneration; however, preserving the differentiated state, which ensures the ability to regenerate damaged cartilage, represents the main challenge during in vitro culturing.
32806778	0	33	theme	Injectable	14:23	arg1	Hydrogel					47:54	Injectable Marine Collagen-Based Hydrogel	14:54	Injectable Marine Collagen-Based Hydrogel	14:54	Production of Injectable Marine Collagen-Based Hydrogel for the Maintenance of Differentiated Chondrocytes in Tissue Engineering Applications.
32806778	5	34	theme	hydrogel	977:984	arg1	stiffness					986:994	the hydrogel stiffness	973:994	the hydrogel stiffness merely varying the concentration of H2O2	973:1035	Moreover, it enables the modulation of the hydrogel stiffness merely varying the concentration of H2O2 without changes in the concentration of polymer precursors.
32806778	7	35	theme	gene	1407:1410	arg1	program					1423:1429	the chondrogenic gene expression program	1390:1429	the chondrogenic gene expression program	1390:1429	Additionally, gene expression profiling of differentiation/dedifferentiation markers provided evidence for the promotion of the chondrogenic gene expression program.
32806778	0	36	theme	Collagen-Based	32:45	arg1	Hydrogel					47:54	Injectable Marine Collagen-Based Hydrogel	14:54	Injectable Marine Collagen-Based Hydrogel	14:54	Production of Injectable Marine Collagen-Based Hydrogel for the Maintenance of Differentiated Chondrocytes in Tissue Engineering Applications.
32806778	2	37	dep	in	456:457	arg1	vitro					459:463	vitro	459:463	vitro	459:463	The use of autologous chondrocyte transplants represent an effective strategy for cell regeneration; however, preserving the differentiated state, which ensures the ability to regenerate damaged cartilage, represents the main challenge during in vitro culturing.
32806778	3	38	theme	injectable	509:518	arg1	hydrogel					542:549	an injectable marine collagen-based hydrogel	506:549	an injectable marine collagen-based hydrogel	506:549	For this purpose, we produced an injectable marine collagen-based hydrogel, by mixing native collagen from the jellyfish Rhizostoma pulmo with hydroxy-phenyl-propionic acid (HPA)-functionalized marine gelatin.
32806778	2	39	theme	main	434:437	arg1	challenge					439:447	the main challenge	430:447	the main challenge during in vitro culturing	430:473	The use of autologous chondrocyte transplants represent an effective strategy for cell regeneration; however, preserving the differentiated state, which ensures the ability to regenerate damaged cartilage, represents the main challenge during in vitro culturing.
32806778	4	40	theme	trap	844:847	arg1	cells					849:853	trap cells	844:853	trap cells	844:853	This biocompatible hydrogel formulation, due to the ability of enzymatically reticulate using horseradish peroxidase (HPR) and H2O2, gives the possibility of trap cells inside, in the absence of cytotoxic effects, during the cross-linking process.
32806778	2	41	theme	damaged	400:406	arg1	cartilage					408:416	damaged cartilage	400:416	damaged cartilage	400:416	The use of autologous chondrocyte transplants represent an effective strategy for cell regeneration; however, preserving the differentiated state, which ensures the ability to regenerate damaged cartilage, represents the main challenge during in vitro culturing.
32806778	3	42	theme	collagen-based	527:540	arg1	hydrogel					542:549	an injectable marine collagen-based hydrogel	506:549	an injectable marine collagen-based hydrogel	506:549	For this purpose, we produced an injectable marine collagen-based hydrogel, by mixing native collagen from the jellyfish Rhizostoma pulmo with hydroxy-phenyl-propionic acid (HPA)-functionalized marine gelatin.
32806778	4	43	theme	effects	891:897	arg1	absence					870:876	the absence	866:876	the absence of cytotoxic effects	866:897	This biocompatible hydrogel formulation, due to the ability of enzymatically reticulate using horseradish peroxidase (HPR) and H2O2, gives the possibility of trap cells inside, in the absence of cytotoxic effects, during the cross-linking process.
32806778	7	44	theme	differentiation/dedifferentiation	1309:1341	arg1	markers					1343:1349	differentiation/dedifferentiation markers	1309:1349	differentiation/dedifferentiation markers	1309:1349	Additionally, gene expression profiling of differentiation/dedifferentiation markers provided evidence for the promotion of the chondrogenic gene expression program.
32806778	8	45	from	practices	1653:1661	arg1	aim					1586:1588	the aim	1582:1588	the aim of the use of autologous chondrocytes in regenerative medicine practices	1582:1661	This, combined with the biochemical properties of marine collagen, represents a promising strategy for maintaining in vitro the cellular phenotype in the aim of the use of autologous chondrocytes in regenerative medicine practices.
32806778	6	46	theme	production	1224:1233	arg1	analysis					1192:1199	morphological analysis	1178:1199	morphological analysis of cell phenotype, GAG production and cytoskeleton organization	1178:1263	The maintenance of differentiated chondrocytes in culture was then evaluated via morphological analysis of cell phenotype, GAG production and cytoskeleton organization.
32806778	4	47	theme	hydrogel	705:712	arg1	formulation					714:724	This biocompatible hydrogel formulation	686:724	This biocompatible hydrogel formulation	686:724	This biocompatible hydrogel formulation, due to the ability of enzymatically reticulate using horseradish peroxidase (HPR) and H2O2, gives the possibility of trap cells inside, in the absence of cytotoxic effects, during the cross-linking process.
32806778	7	48	theme	gene	1280:1283	arg1	profiling					1296:1304	gene expression profiling	1280:1304	gene expression profiling of differentiation/dedifferentiation markers	1280:1349	Additionally, gene expression profiling of differentiation/dedifferentiation markers provided evidence for the promotion of the chondrogenic gene expression program.
32806778	3	49	theme	Rhizostoma	597:606	arg1	pulmo					608:612	the jellyfish Rhizostoma pulmo	583:612	the jellyfish Rhizostoma pulmo with hydroxy-phenyl-propionic acid (HPA)-functionalized marine gelatin	583:683	For this purpose, we produced an injectable marine collagen-based hydrogel, by mixing native collagen from the jellyfish Rhizostoma pulmo with hydroxy-phenyl-propionic acid (HPA)-functionalized marine gelatin.
32806778	1	50	theme	self-repair	200:210	arg1	ability					189:195	limited ability	181:195	limited ability of self-repair	181:210	Cartilage is an avascular tissue with limited ability of self-repair.
32806778	6	51	theme	cell	1204:1207	arg1	phenotype					1209:1217	cell phenotype	1204:1217	cell phenotype	1204:1217	The maintenance of differentiated chondrocytes in culture was then evaluated via morphological analysis of cell phenotype, GAG production and cytoskeleton organization.
32806778	0	52	from	Chondrocytes	94:105	arg1	Applications					129:140	Tissue Engineering Applications	110:140	Tissue Engineering Applications	110:140	Production of Injectable Marine Collagen-Based Hydrogel for the Maintenance of Differentiated Chondrocytes in Tissue Engineering Applications.
32806778	3	53	theme	acid	644:647	arg1	gelatin					677:683	hydroxy-phenyl-propionic acid (HPA)-functionalized marine gelatin	619:683	hydroxy-phenyl-propionic acid (HPA)-functionalized marine gelatin	619:683	For this purpose, we produced an injectable marine collagen-based hydrogel, by mixing native collagen from the jellyfish Rhizostoma pulmo with hydroxy-phenyl-propionic acid (HPA)-functionalized marine gelatin.
32806778	8	54	theme	cellular	1560:1567	arg1	phenotype					1569:1577	the cellular phenotype	1556:1577	the cellular phenotype in the aim of the use of autologous chondrocytes in regenerative medicine practices	1556:1661	This, combined with the biochemical properties of marine collagen, represents a promising strategy for maintaining in vitro the cellular phenotype in the aim of the use of autologous chondrocytes in regenerative medicine practices.
32806778	2	55	dep	represent	259:267	arg1	preserving					323:332	preserving	323:332	preserving the differentiated state, which ensures the ability to regenerate damaged cartilage, represents the main challenge during in vitro culturing	323:473	The use of autologous chondrocyte transplants represent an effective strategy for cell regeneration; however, preserving the differentiated state, which ensures the ability to regenerate damaged cartilage, represents the main challenge during in vitro culturing.
32806778	8	56	from	aim	1586:1588	arg1	practices					1653:1661	regenerative medicine practices	1631:1661	regenerative medicine practices	1631:1661	This, combined with the biochemical properties of marine collagen, represents a promising strategy for maintaining in vitro the cellular phenotype in the aim of the use of autologous chondrocytes in regenerative medicine practices.
32806778	2	57	theme	transplants	247:257	arg1	use					217:219	The use	213:219	The use of autologous chondrocyte transplants	213:257	The use of autologous chondrocyte transplants represent an effective strategy for cell regeneration; however, preserving the differentiated state, which ensures the ability to regenerate damaged cartilage, represents the main challenge during in vitro culturing.
32806778	4	58	theme	cross-linking	911:923	arg1	process					925:931	the cross-linking process	907:931	the cross-linking process	907:931	This biocompatible hydrogel formulation, due to the ability of enzymatically reticulate using horseradish peroxidase (HPR) and H2O2, gives the possibility of trap cells inside, in the absence of cytotoxic effects, during the cross-linking process.
32806778	0	59	from	Applications	129:140	arg1	Maintenance					64:74	the Maintenance	60:74	the Maintenance of Differentiated Chondrocytes in Tissue Engineering Applications	60:140	Production of Injectable Marine Collagen-Based Hydrogel for the Maintenance of Differentiated Chondrocytes in Tissue Engineering Applications.
32806778	5	60	theme	polymer	1077:1083	arg1	precursors					1085:1094	polymer precursors	1077:1094	polymer precursors	1077:1094	Moreover, it enables the modulation of the hydrogel stiffness merely varying the concentration of H2O2 without changes in the concentration of polymer precursors.
32806778	3	61	theme	-functionalized	654:668	arg1	gelatin					677:683	hydroxy-phenyl-propionic acid (HPA)-functionalized marine gelatin	619:683	hydroxy-phenyl-propionic acid (HPA)-functionalized marine gelatin	619:683	For this purpose, we produced an injectable marine collagen-based hydrogel, by mixing native collagen from the jellyfish Rhizostoma pulmo with hydroxy-phenyl-propionic acid (HPA)-functionalized marine gelatin.
32806778	4	62	theme	using	774:778	arg1	HPR					804:806	HPR	804:806	HPR	804:806	This biocompatible hydrogel formulation, due to the ability of enzymatically reticulate using horseradish peroxidase (HPR) and H2O2, gives the possibility of trap cells inside, in the absence of cytotoxic effects, during the cross-linking process.
32806778	4	62	theme	using	774:778	arg1	peroxidase					792:801	using horseradish peroxidase	774:801	using horseradish peroxidase (HPR)	774:807	This biocompatible hydrogel formulation, due to the ability of enzymatically reticulate using horseradish peroxidase (HPR) and H2O2, gives the possibility of trap cells inside, in the absence of cytotoxic effects, during the cross-linking process.
32806778	7	63	theme	expression	1285:1294	arg1	profiling					1296:1304	gene expression profiling	1280:1304	gene expression profiling of differentiation/dedifferentiation markers	1280:1349	Additionally, gene expression profiling of differentiation/dedifferentiation markers provided evidence for the promotion of the chondrogenic gene expression program.
32806778	3	64	theme	HPA	650:652	arg1	gelatin					677:683	hydroxy-phenyl-propionic acid (HPA)-functionalized marine gelatin	619:683	hydroxy-phenyl-propionic acid (HPA)-functionalized marine gelatin	619:683	For this purpose, we produced an injectable marine collagen-based hydrogel, by mixing native collagen from the jellyfish Rhizostoma pulmo with hydroxy-phenyl-propionic acid (HPA)-functionalized marine gelatin.
32806778	1	65	theme	avascular	159:167	arg1	tissue					169:174	an avascular tissue	156:174	an avascular tissue with limited ability of self-repair	156:210	Cartilage is an avascular tissue with limited ability of self-repair.
32806778	1	65	theme	avascular	159:167	arg1	Cartilage					143:151	Cartilage	143:151	Cartilage	143:151	Cartilage is an avascular tissue with limited ability of self-repair.
32806778	6	66	theme	cytoskeleton	1239:1250	arg1	organization					1252:1263	cytoskeleton organization	1239:1263	cytoskeleton organization	1239:1263	The maintenance of differentiated chondrocytes in culture was then evaluated via morphological analysis of cell phenotype, GAG production and cytoskeleton organization.
32806778	0	67	theme	Chondrocytes	94:105	arg1	Maintenance					64:74	the Maintenance	60:74	the Maintenance of Differentiated Chondrocytes in Tissue Engineering Applications	60:140	Production of Injectable Marine Collagen-Based Hydrogel for the Maintenance of Differentiated Chondrocytes in Tissue Engineering Applications.
32806778	0	68	theme	Tissue	110:115	arg1	Applications					129:140	Tissue Engineering Applications	110:140	Tissue Engineering Applications	110:140	Production of Injectable Marine Collagen-Based Hydrogel for the Maintenance of Differentiated Chondrocytes in Tissue Engineering Applications.
32806778	5	69	theme	stiffness	986:994	arg1	modulation					959:968	the modulation	955:968	the modulation of the hydrogel stiffness merely varying the concentration of H2O2	955:1035	Moreover, it enables the modulation of the hydrogel stiffness merely varying the concentration of H2O2 without changes in the concentration of polymer precursors.
32806778	3	70	theme	jellyfish	587:595	arg1	pulmo					608:612	the jellyfish Rhizostoma pulmo	583:612	the jellyfish Rhizostoma pulmo with hydroxy-phenyl-propionic acid (HPA)-functionalized marine gelatin	583:683	For this purpose, we produced an injectable marine collagen-based hydrogel, by mixing native collagen from the jellyfish Rhizostoma pulmo with hydroxy-phenyl-propionic acid (HPA)-functionalized marine gelatin.
32806778	8	71	theme	chondrocytes	1615:1626	arg1	use					1597:1599	the use	1593:1599	the use of autologous chondrocytes in regenerative medicine practices	1593:1661	This, combined with the biochemical properties of marine collagen, represents a promising strategy for maintaining in vitro the cellular phenotype in the aim of the use of autologous chondrocytes in regenerative medicine practices.
32806778	6	72	theme	differentiated	1116:1129	arg1	chondrocytes					1131:1142	differentiated chondrocytes	1116:1142	differentiated chondrocytes	1116:1142	The maintenance of differentiated chondrocytes in culture was then evaluated via morphological analysis of cell phenotype, GAG production and cytoskeleton organization.
32806778	8	73	theme	regenerative	1631:1642	arg1	practices					1653:1661	regenerative medicine practices	1631:1661	regenerative medicine practices	1631:1661	This, combined with the biochemical properties of marine collagen, represents a promising strategy for maintaining in vitro the cellular phenotype in the aim of the use of autologous chondrocytes in regenerative medicine practices.
32806778	7	74	theme	expression	1412:1421	arg1	program					1423:1429	the chondrogenic gene expression program	1390:1429	the chondrogenic gene expression program	1390:1429	Additionally, gene expression profiling of differentiation/dedifferentiation markers provided evidence for the promotion of the chondrogenic gene expression program.
32806778	4	75	theme	peroxidase	792:801	arg1	ability					738:744	the ability	734:744	the ability of enzymatically reticulate using horseradish peroxidase (HPR) and H2O2	734:816	This biocompatible hydrogel formulation, due to the ability of enzymatically reticulate using horseradish peroxidase (HPR) and H2O2, gives the possibility of trap cells inside, in the absence of cytotoxic effects, during the cross-linking process.
32806778	0	76	theme	Marine	25:30	arg1	Hydrogel					47:54	Injectable Marine Collagen-Based Hydrogel	14:54	Injectable Marine Collagen-Based Hydrogel	14:54	Production of Injectable Marine Collagen-Based Hydrogel for the Maintenance of Differentiated Chondrocytes in Tissue Engineering Applications.
32806778	7	77	theme	chondrogenic	1394:1405	arg1	program					1423:1429	the chondrogenic gene expression program	1390:1429	the chondrogenic gene expression program	1390:1429	Additionally, gene expression profiling of differentiation/dedifferentiation markers provided evidence for the promotion of the chondrogenic gene expression program.
32806778	0	78	theme	Hydrogel	47:54	arg1	Production					0:9	Production	0:9	Production of Injectable Marine Collagen-Based Hydrogel for the Maintenance of Differentiated Chondrocytes in Tissue Engineering Applications.	0:141	Production of Injectable Marine Collagen-Based Hydrogel for the Maintenance of Differentiated Chondrocytes in Tissue Engineering Applications.
32806778	8	79	theme	collagen	1489:1496	arg1	properties					1468:1477	the biochemical properties	1452:1477	the biochemical properties of marine collagen	1452:1496	This, combined with the biochemical properties of marine collagen, represents a promising strategy for maintaining in vitro the cellular phenotype in the aim of the use of autologous chondrocytes in regenerative medicine practices.
32806778	8	80	from	phenotype	1569:1577	arg1	aim					1586:1588	the aim	1582:1588	the aim of the use of autologous chondrocytes in regenerative medicine practices	1582:1661	This, combined with the biochemical properties of marine collagen, represents a promising strategy for maintaining in vitro the cellular phenotype in the aim of the use of autologous chondrocytes in regenerative medicine practices.
32806778	6	81	theme	morphological	1178:1190	arg1	analysis					1192:1199	morphological analysis	1178:1199	morphological analysis of cell phenotype, GAG production and cytoskeleton organization	1178:1263	The maintenance of differentiated chondrocytes in culture was then evaluated via morphological analysis of cell phenotype, GAG production and cytoskeleton organization.
32806778	2	82	theme	autologous	224:233	arg1	transplants					247:257	autologous chondrocyte transplants	224:257	autologous chondrocyte transplants	224:257	The use of autologous chondrocyte transplants represent an effective strategy for cell regeneration; however, preserving the differentiated state, which ensures the ability to regenerate damaged cartilage, represents the main challenge during in vitro culturing.
32806778	3	83	theme	marine	520:525	arg1	hydrogel					542:549	an injectable marine collagen-based hydrogel	506:549	an injectable marine collagen-based hydrogel	506:549	For this purpose, we produced an injectable marine collagen-based hydrogel, by mixing native collagen from the jellyfish Rhizostoma pulmo with hydroxy-phenyl-propionic acid (HPA)-functionalized marine gelatin.
32806778	8	84	theme	promising	1512:1520	arg1	strategy					1522:1529	a promising strategy	1510:1529	a promising strategy for maintaining in vitro the cellular phenotype in the aim of the use of autologous chondrocytes in regenerative medicine practices	1510:1661	This, combined with the biochemical properties of marine collagen, represents a promising strategy for maintaining in vitro the cellular phenotype in the aim of the use of autologous chondrocytes in regenerative medicine practices.
32806778	5	85	theme	H2O2	1032:1035	arg1	concentration					1015:1027	the concentration	1011:1027	the concentration of H2O2	1011:1035	Moreover, it enables the modulation of the hydrogel stiffness merely varying the concentration of H2O2 without changes in the concentration of polymer precursors.
32806778	7	86	theme	markers	1343:1349	arg1	profiling					1296:1304	gene expression profiling	1280:1304	gene expression profiling of differentiation/dedifferentiation markers	1280:1349	Additionally, gene expression profiling of differentiation/dedifferentiation markers provided evidence for the promotion of the chondrogenic gene expression program.
32806778	3	87	with	pulmo	608:612	arg1	gelatin					677:683	hydroxy-phenyl-propionic acid (HPA)-functionalized marine gelatin	619:683	hydroxy-phenyl-propionic acid (HPA)-functionalized marine gelatin	619:683	For this purpose, we produced an injectable marine collagen-based hydrogel, by mixing native collagen from the jellyfish Rhizostoma pulmo with hydroxy-phenyl-propionic acid (HPA)-functionalized marine gelatin.
34789635	0	0	theme	Transient	80:88	arg1	Attack					99:104	Ischemic Stroke or Transient Ischemic Attack	61:104	Ischemic Stroke or Transient Ischemic Attack	61:104	Bridging Therapy With Heparin Before Starting Rivaroxaban in Ischemic Stroke or Transient Ischemic Attack With Non-Valvular Atrial Fibrillation.
34789635	3	1	dep	days	746:749	arg1	onset					757:761	onset	757:761	onset	757:761	Outcome measures were ischemic events, major bleeding, their composite, and death or disability 90 days after onset.
34789635	1	2	from	attack	353:358	arg1	patients					369:376	patients	369:376	patients with non-valvular atrial fibrillation (NVAF)	369:421	BACKGROUND The present observational study aimed to clarify the association between bridging therapy with heparin before starting rivaroxaban and clinical outcomes after ischemic stroke or transient ischemic attack (TIA) in patients with non-valvular atrial fibrillation (NVAF).
34789635	1	3	theme	observational	168:180	arg1	study					182:186	The present observational study	156:186	The present observational study	156:186	BACKGROUND The present observational study aimed to clarify the association between bridging therapy with heparin before starting rivaroxaban and clinical outcomes after ischemic stroke or transient ischemic attack (TIA) in patients with non-valvular atrial fibrillation (NVAF).
34789635	1	4	with	patients	369:376	arg1	NVAF					417:420	NVAF	417:420	NVAF	417:420	BACKGROUND The present observational study aimed to clarify the association between bridging therapy with heparin before starting rivaroxaban and clinical outcomes after ischemic stroke or transient ischemic attack (TIA) in patients with non-valvular atrial fibrillation (NVAF).
34789635	1	4	with	patients	369:376	arg1	fibrillation					403:414	non-valvular atrial fibrillation	383:414	non-valvular atrial fibrillation (NVAF)	383:421	BACKGROUND The present observational study aimed to clarify the association between bridging therapy with heparin before starting rivaroxaban and clinical outcomes after ischemic stroke or transient ischemic attack (TIA) in patients with non-valvular atrial fibrillation (NVAF).
34789635	8	5	from	patients	1489:1496	arg1	frequent					1477:1484	frequent	1477:1484	frequent	1477:1484	CONCLUSIONS The composite of ischemic events and major bleeding was more frequent in patients with NVAF who received bridging therapy with low-dose heparin than in those who started treatment directly with rivaroxaban after ischemic stroke or TIA.
34789635	5	6	with	therapy	891:897	arg1	heparin					943:949	unfractionated or low-molecular-weight heparin	904:949	heparin	943:949	Of 1,308 analyzed patients, 638 received bridging therapy with unfractionated or low-molecular-weight heparin with a median of 10,000 IU/day.
34789635	5	6	with	therapy	891:897	arg1	median					958:963	a median	956:963	a median of 10,000 IU/day	956:980	Of 1,308 analyzed patients, 638 received bridging therapy with unfractionated or low-molecular-weight heparin with a median of 10,000 IU/day.
34789635	5	7	theme	bridging	882:889	arg1	therapy					891:897	bridging therapy	882:897	bridging therapy with unfractionated or low-molecular-weight heparin with a median of 10,000 IU/day	882:980	Of 1,308 analyzed patients, 638 received bridging therapy with unfractionated or low-molecular-weight heparin with a median of 10,000 IU/day.
34789635	6	8	theme	bridging	1004:1011	arg1	therapy					1013:1019	bridging therapy	1004:1019	bridging therapy	1004:1019	Associations between bridging therapy and ischemic events or major bleeding were not statistically significant individually, but the association between bridging therapy and their composite was statistically significant (multivariable-adjusted hazard ratio, 1.80; 95% confidence interval, 1.01-3.29).
34789635	8	9	theme	low-dose	1543:1550	arg1	heparin					1552:1558	low-dose heparin	1543:1558	low-dose heparin	1543:1558	CONCLUSIONS The composite of ischemic events and major bleeding was more frequent in patients with NVAF who received bridging therapy with low-dose heparin than in those who started treatment directly with rivaroxaban after ischemic stroke or TIA.
34789635	8	10	with	treatment	1586:1594	arg1	rivaroxaban					1610:1620	rivaroxaban	1610:1620	rivaroxaban	1610:1620	CONCLUSIONS The composite of ischemic events and major bleeding was more frequent in patients with NVAF who received bridging therapy with low-dose heparin than in those who started treatment directly with rivaroxaban after ischemic stroke or TIA.
34789635	4	11	theme	Ischemic	764:771	arg1	TIA					813:815	TIA	813:815	TIA	813:815	Ischemic events were defined as ischemic stroke, TIA, and systemic embolism.
34789635	4	11	theme	Ischemic	764:771	arg1	stroke					805:810	ischemic stroke	796:810	ischemic stroke	796:810	Ischemic events were defined as ischemic stroke, TIA, and systemic embolism.
34789635	4	11	theme	Ischemic	764:771	arg1	events					773:778	Ischemic events	764:778	Ischemic events	764:778	Ischemic events were defined as ischemic stroke, TIA, and systemic embolism.
34789635	4	11	theme	Ischemic	764:771	arg1	embolism					831:838	systemic embolism	822:838	systemic embolism	822:838	Ischemic events were defined as ischemic stroke, TIA, and systemic embolism.
34789635	1	12	theme	clinical	291:298	arg1	outcomes					300:307	clinical outcomes	291:307	clinical outcomes	291:307	BACKGROUND The present observational study aimed to clarify the association between bridging therapy with heparin before starting rivaroxaban and clinical outcomes after ischemic stroke or transient ischemic attack (TIA) in patients with non-valvular atrial fibrillation (NVAF).
34789635	0	13	theme	Ischemic	90:97	arg1	Attack					99:104	Ischemic Stroke or Transient Ischemic Attack	61:104	Ischemic Stroke or Transient Ischemic Attack	61:104	Bridging Therapy With Heparin Before Starting Rivaroxaban in Ischemic Stroke or Transient Ischemic Attack With Non-Valvular Atrial Fibrillation.
34789635	6	14	theme	hazard	1227:1232	arg1	association					1116:1126	the association	1112:1126	the association between bridging therapy	1112:1151	Associations between bridging therapy and ischemic events or major bleeding were not statistically significant individually, but the association between bridging therapy and their composite was statistically significant (multivariable-adjusted hazard ratio, 1.80; 95% confidence interval, 1.01-3.29).
34789635	6	14	theme	hazard	1227:1232	arg1	significant					1191:1201	significant	1191:1201	significant	1191:1201	Associations between bridging therapy and ischemic events or major bleeding were not statistically significant individually, but the association between bridging therapy and their composite was statistically significant (multivariable-adjusted hazard ratio, 1.80; 95% confidence interval, 1.01-3.29).
34789635	6	14	theme	hazard	1227:1232	arg1	ratio					1234:1238	multivariable-adjusted hazard ratio	1204:1238	multivariable-adjusted hazard ratio	1204:1238	Associations between bridging therapy and ischemic events or major bleeding were not statistically significant individually, but the association between bridging therapy and their composite was statistically significant (multivariable-adjusted hazard ratio, 1.80; 95% confidence interval, 1.01-3.29).
34789635	1	15	dep	BACKGROUND	145:154	arg1	aimed					188:192	aimed	188:192	aimed to clarify the association between bridging therapy with heparin before starting rivaroxaban and clinical outcomes after ischemic stroke or transient ischemic attack (TIA) in patients with non-valvular atrial fibrillation (NVAF)	188:421	BACKGROUND The present observational study aimed to clarify the association between bridging therapy with heparin before starting rivaroxaban and clinical outcomes after ischemic stroke or transient ischemic attack (TIA) in patients with non-valvular atrial fibrillation (NVAF).
34789635	6	16	theme	bridging	1136:1143	arg1	therapy					1145:1151	bridging therapy	1136:1151	bridging therapy	1136:1151	Associations between bridging therapy and ischemic events or major bleeding were not statistically significant individually, but the association between bridging therapy and their composite was statistically significant (multivariable-adjusted hazard ratio, 1.80; 95% confidence interval, 1.01-3.29).
34789635	0	17	theme	Non-Valvular	111:122	arg1	Fibrillation					131:142	Non-Valvular Atrial Fibrillation	111:142	Non-Valvular Atrial Fibrillation	111:142	Bridging Therapy With Heparin Before Starting Rivaroxaban in Ischemic Stroke or Transient Ischemic Attack With Non-Valvular Atrial Fibrillation.
34789635	1	18	theme	non-valvular	383:394	arg1	NVAF					417:420	NVAF	417:420	NVAF	417:420	BACKGROUND The present observational study aimed to clarify the association between bridging therapy with heparin before starting rivaroxaban and clinical outcomes after ischemic stroke or transient ischemic attack (TIA) in patients with non-valvular atrial fibrillation (NVAF).
34789635	1	18	theme	non-valvular	383:394	arg1	fibrillation					403:414	non-valvular atrial fibrillation	383:414	non-valvular atrial fibrillation (NVAF)	383:421	BACKGROUND The present observational study aimed to clarify the association between bridging therapy with heparin before starting rivaroxaban and clinical outcomes after ischemic stroke or transient ischemic attack (TIA) in patients with non-valvular atrial fibrillation (NVAF).
34789635	5	19	theme	IU/day	975:980	arg1	median					958:963	a median	956:963	a median of 10,000 IU/day	956:980	Of 1,308 analyzed patients, 638 received bridging therapy with unfractionated or low-molecular-weight heparin with a median of 10,000 IU/day.
34789635	8	20	dep	CONCLUSIONS	1404:1414	arg1	events					1442:1447	ischemic events	1433:1447	ischemic events	1433:1447	CONCLUSIONS The composite of ischemic events and major bleeding was more frequent in patients with NVAF who received bridging therapy with low-dose heparin than in those who started treatment directly with rivaroxaban after ischemic stroke or TIA.
34789635	8	20	dep	CONCLUSIONS	1404:1414	arg1	bleeding					1459:1466	bleeding	1459:1466	bleeding	1459:1466	CONCLUSIONS The composite of ischemic events and major bleeding was more frequent in patients with NVAF who received bridging therapy with low-dose heparin than in those who started treatment directly with rivaroxaban after ischemic stroke or TIA.
34789635	8	20	dep	CONCLUSIONS	1404:1414	arg1	composite					1420:1428	The composite	1416:1428	The composite of ischemic events	1416:1447	CONCLUSIONS The composite of ischemic events and major bleeding was more frequent in patients with NVAF who received bridging therapy with low-dose heparin than in those who started treatment directly with rivaroxaban after ischemic stroke or TIA.
34789635	2	21	theme	artery	531:536	arg1	territory					538:546	the middle cerebral artery territory	511:546	the middle cerebral artery territory	511:546	Methods and Results: Patients with NVAF who experienced acute ischemic stroke or TIA of the middle cerebral artery territory and started rivaroxaban within 30 days after onset were enrolled and were followed up for 90 days.
34789635	1	22	theme	atrial	396:401	arg1	NVAF					417:420	NVAF	417:420	NVAF	417:420	BACKGROUND The present observational study aimed to clarify the association between bridging therapy with heparin before starting rivaroxaban and clinical outcomes after ischemic stroke or transient ischemic attack (TIA) in patients with non-valvular atrial fibrillation (NVAF).
34789635	1	22	theme	atrial	396:401	arg1	fibrillation					403:414	non-valvular atrial fibrillation	383:414	non-valvular atrial fibrillation (NVAF)	383:421	BACKGROUND The present observational study aimed to clarify the association between bridging therapy with heparin before starting rivaroxaban and clinical outcomes after ischemic stroke or transient ischemic attack (TIA) in patients with non-valvular atrial fibrillation (NVAF).
34789635	4	23	theme	systemic	822:829	arg1	events					773:778	Ischemic events	764:778	Ischemic events	764:778	Ischemic events were defined as ischemic stroke, TIA, and systemic embolism.
34789635	4	23	theme	systemic	822:829	arg1	embolism					831:838	systemic embolism	822:838	systemic embolism	822:838	Ischemic events were defined as ischemic stroke, TIA, and systemic embolism.
34789635	2	24	theme	territory	538:546	arg1	stroke					494:499	acute ischemic stroke	479:499	acute ischemic stroke	479:499	Methods and Results: Patients with NVAF who experienced acute ischemic stroke or TIA of the middle cerebral artery territory and started rivaroxaban within 30 days after onset were enrolled and were followed up for 90 days.
34789635	2	24	theme	territory	538:546	arg1	TIA					504:506	TIA	504:506	TIA of the middle cerebral artery territory	504:546	Methods and Results: Patients with NVAF who experienced acute ischemic stroke or TIA of the middle cerebral artery territory and started rivaroxaban within 30 days after onset were enrolled and were followed up for 90 days.
34789635	2	25	theme	acute	479:483	arg1	stroke					494:499	acute ischemic stroke	479:499	acute ischemic stroke	479:499	Methods and Results: Patients with NVAF who experienced acute ischemic stroke or TIA of the middle cerebral artery territory and started rivaroxaban within 30 days after onset were enrolled and were followed up for 90 days.
34789635	2	26	with	Patients	444:451	arg1	NVAF					458:461	NVAF	458:461	NVAF	458:461	Methods and Results: Patients with NVAF who experienced acute ischemic stroke or TIA of the middle cerebral artery territory and started rivaroxaban within 30 days after onset were enrolled and were followed up for 90 days.
34789635	2	27	theme	cerebral	522:529	arg1	territory					538:546	the middle cerebral artery territory	511:546	the middle cerebral artery territory	511:546	Methods and Results: Patients with NVAF who experienced acute ischemic stroke or TIA of the middle cerebral artery territory and started rivaroxaban within 30 days after onset were enrolled and were followed up for 90 days.
34789635	0	28	from	Rivaroxaban	46:56	arg1	Attack					99:104	Ischemic Stroke or Transient Ischemic Attack	61:104	Ischemic Stroke or Transient Ischemic Attack	61:104	Bridging Therapy With Heparin Before Starting Rivaroxaban in Ischemic Stroke or Transient Ischemic Attack With Non-Valvular Atrial Fibrillation.
34789635	6	29	dep	significant	1191:1201	arg1	association					1116:1126	the association	1112:1126	the association between bridging therapy	1112:1151	Associations between bridging therapy and ischemic events or major bleeding were not statistically significant individually, but the association between bridging therapy and their composite was statistically significant (multivariable-adjusted hazard ratio, 1.80; 95% confidence interval, 1.01-3.29).
34789635	6	29	dep	significant	1191:1201	arg1	significant					1191:1201	significant	1191:1201	significant	1191:1201	Associations between bridging therapy and ischemic events or major bleeding were not statistically significant individually, but the association between bridging therapy and their composite was statistically significant (multivariable-adjusted hazard ratio, 1.80; 95% confidence interval, 1.01-3.29).
34789635	6	29	dep	significant	1191:1201	arg1	ratio					1234:1238	multivariable-adjusted hazard ratio	1204:1238	multivariable-adjusted hazard ratio	1204:1238	Associations between bridging therapy and ischemic events or major bleeding were not statistically significant individually, but the association between bridging therapy and their composite was statistically significant (multivariable-adjusted hazard ratio, 1.80; 95% confidence interval, 1.01-3.29).
34789635	8	30	with	therapy	1530:1536	arg1	heparin					1552:1558	low-dose heparin	1543:1558	low-dose heparin	1543:1558	CONCLUSIONS The composite of ischemic events and major bleeding was more frequent in patients with NVAF who received bridging therapy with low-dose heparin than in those who started treatment directly with rivaroxaban after ischemic stroke or TIA.
34789635	1	31	theme	ischemic	315:322	arg1	TIA					361:363	TIA	361:363	TIA	361:363	BACKGROUND The present observational study aimed to clarify the association between bridging therapy with heparin before starting rivaroxaban and clinical outcomes after ischemic stroke or transient ischemic attack (TIA) in patients with non-valvular atrial fibrillation (NVAF).
34789635	1	31	theme	ischemic	315:322	arg1	attack					353:358	ischemic stroke or transient ischemic attack	315:358	ischemic stroke or transient ischemic attack (TIA) in patients with non-valvular atrial fibrillation (NVAF)	315:421	BACKGROUND The present observational study aimed to clarify the association between bridging therapy with heparin before starting rivaroxaban and clinical outcomes after ischemic stroke or transient ischemic attack (TIA) in patients with non-valvular atrial fibrillation (NVAF).
34789635	8	32	theme	events	1442:1447	arg1	events					1442:1447	ischemic events	1433:1447	ischemic events	1433:1447	CONCLUSIONS The composite of ischemic events and major bleeding was more frequent in patients with NVAF who received bridging therapy with low-dose heparin than in those who started treatment directly with rivaroxaban after ischemic stroke or TIA.
34789635	8	32	theme	events	1442:1447	arg1	composite					1420:1428	The composite	1416:1428	The composite of ischemic events	1416:1447	CONCLUSIONS The composite of ischemic events and major bleeding was more frequent in patients with NVAF who received bridging therapy with low-dose heparin than in those who started treatment directly with rivaroxaban after ischemic stroke or TIA.
34789635	8	33	from	frequent	1477:1484	arg1	patients					1489:1496	patients	1489:1496	patients with NVAF who received bridging therapy with low-dose heparin than in those who started treatment directly with rivaroxaban after ischemic stroke or TIA	1489:1649	CONCLUSIONS The composite of ischemic events and major bleeding was more frequent in patients with NVAF who received bridging therapy with low-dose heparin than in those who started treatment directly with rivaroxaban after ischemic stroke or TIA.
34789635	1	34	theme	stroke	324:329	arg1	TIA					361:363	TIA	361:363	TIA	361:363	BACKGROUND The present observational study aimed to clarify the association between bridging therapy with heparin before starting rivaroxaban and clinical outcomes after ischemic stroke or transient ischemic attack (TIA) in patients with non-valvular atrial fibrillation (NVAF).
34789635	1	34	theme	stroke	324:329	arg1	attack					353:358	ischemic stroke or transient ischemic attack	315:358	ischemic stroke or transient ischemic attack (TIA) in patients with non-valvular atrial fibrillation (NVAF)	315:421	BACKGROUND The present observational study aimed to clarify the association between bridging therapy with heparin before starting rivaroxaban and clinical outcomes after ischemic stroke or transient ischemic attack (TIA) in patients with non-valvular atrial fibrillation (NVAF).
34789635	0	35	theme	Atrial	124:129	arg1	Fibrillation					131:142	Non-Valvular Atrial Fibrillation	111:142	Non-Valvular Atrial Fibrillation	111:142	Bridging Therapy With Heparin Before Starting Rivaroxaban in Ischemic Stroke or Transient Ischemic Attack With Non-Valvular Atrial Fibrillation.
34789635	6	36	dep	ratio	1234:1238	arg1	1.01-3.29					1272:1280	1.01-3.29	1272:1280	1.01-3.29	1272:1280	Associations between bridging therapy and ischemic events or major bleeding were not statistically significant individually, but the association between bridging therapy and their composite was statistically significant (multivariable-adjusted hazard ratio, 1.80; 95% confidence interval, 1.01-3.29).
34789635	6	36	dep	ratio	1234:1238	arg1	interval					1262:1269	95% confidence interval	1247:1269	95% confidence interval	1247:1269	Associations between bridging therapy and ischemic events or major bleeding were not statistically significant individually, but the association between bridging therapy and their composite was statistically significant (multivariable-adjusted hazard ratio, 1.80; 95% confidence interval, 1.01-3.29).
34789635	7	37	theme	disability	1338:1347	arg1	days					1352:1355	bridging therapy and death or disability 90 days	1308:1355	days	1352:1355	The association between bridging therapy and death or disability 90 days after onset was not statistically significant.
34789635	8	38	with	patients	1489:1496	arg1	NVAF					1503:1506	NVAF	1503:1506	NVAF	1503:1506	CONCLUSIONS The composite of ischemic events and major bleeding was more frequent in patients with NVAF who received bridging therapy with low-dose heparin than in those who started treatment directly with rivaroxaban after ischemic stroke or TIA.
34789635	1	39	with	association	209:219	arg1	heparin					251:257	heparin	251:257	heparin before starting rivaroxaban and clinical outcomes after ischemic stroke or transient ischemic attack (TIA) in patients with non-valvular atrial fibrillation (NVAF)	251:421	BACKGROUND The present observational study aimed to clarify the association between bridging therapy with heparin before starting rivaroxaban and clinical outcomes after ischemic stroke or transient ischemic attack (TIA) in patients with non-valvular atrial fibrillation (NVAF).
34789635	3	40	theme	ischemic	669:676	arg1	events					678:683	ischemic events	669:683	ischemic events	669:683	Outcome measures were ischemic events, major bleeding, their composite, and death or disability 90 days after onset.
34789635	8	41	theme	ischemic	1433:1440	arg1	events					1442:1447	ischemic events	1433:1447	ischemic events	1433:1447	CONCLUSIONS The composite of ischemic events and major bleeding was more frequent in patients with NVAF who received bridging therapy with low-dose heparin than in those who started treatment directly with rivaroxaban after ischemic stroke or TIA.
34789635	1	42	theme	bridging	229:236	arg1	therapy					238:244	bridging therapy	229:244	bridging therapy	229:244	BACKGROUND The present observational study aimed to clarify the association between bridging therapy with heparin before starting rivaroxaban and clinical outcomes after ischemic stroke or transient ischemic attack (TIA) in patients with non-valvular atrial fibrillation (NVAF).
34789635	1	43	theme	transient	334:342	arg1	TIA					361:363	TIA	361:363	TIA	361:363	BACKGROUND The present observational study aimed to clarify the association between bridging therapy with heparin before starting rivaroxaban and clinical outcomes after ischemic stroke or transient ischemic attack (TIA) in patients with non-valvular atrial fibrillation (NVAF).
34789635	1	43	theme	transient	334:342	arg1	attack					353:358	ischemic stroke or transient ischemic attack	315:358	ischemic stroke or transient ischemic attack (TIA) in patients with non-valvular atrial fibrillation (NVAF)	315:421	BACKGROUND The present observational study aimed to clarify the association between bridging therapy with heparin before starting rivaroxaban and clinical outcomes after ischemic stroke or transient ischemic attack (TIA) in patients with non-valvular atrial fibrillation (NVAF).
34789635	3	44	theme	Outcome	647:653	arg1	measures					655:662	Outcome measures	647:662	Outcome measures	647:662	Outcome measures were ischemic events, major bleeding, their composite, and death or disability 90 days after onset.
34789635	1	45	theme	ischemic	344:351	arg1	TIA					361:363	TIA	361:363	TIA	361:363	BACKGROUND The present observational study aimed to clarify the association between bridging therapy with heparin before starting rivaroxaban and clinical outcomes after ischemic stroke or transient ischemic attack (TIA) in patients with non-valvular atrial fibrillation (NVAF).
34789635	1	45	theme	ischemic	344:351	arg1	attack					353:358	ischemic stroke or transient ischemic attack	315:358	ischemic stroke or transient ischemic attack (TIA) in patients with non-valvular atrial fibrillation (NVAF)	315:421	BACKGROUND The present observational study aimed to clarify the association between bridging therapy with heparin before starting rivaroxaban and clinical outcomes after ischemic stroke or transient ischemic attack (TIA) in patients with non-valvular atrial fibrillation (NVAF).
34789635	2	46	theme	middle	515:520	arg1	territory					538:546	the middle cerebral artery territory	511:546	the middle cerebral artery territory	511:546	Methods and Results: Patients with NVAF who experienced acute ischemic stroke or TIA of the middle cerebral artery territory and started rivaroxaban within 30 days after onset were enrolled and were followed up for 90 days.
34789635	6	47	theme	confidence	1251:1260	arg1	interval					1262:1269	95% confidence interval	1247:1269	95% confidence interval	1247:1269	Associations between bridging therapy and ischemic events or major bleeding were not statistically significant individually, but the association between bridging therapy and their composite was statistically significant (multivariable-adjusted hazard ratio, 1.80; 95% confidence interval, 1.01-3.29).
34789635	7	48	theme	bridging	1308:1315	arg1	therapy					1317:1323	bridging therapy and death or disability 90 days	1308:1355	therapy	1317:1323	The association between bridging therapy and death or disability 90 days after onset was not statistically significant.
34789635	2	49	theme	ischemic	485:492	arg1	stroke					494:499	acute ischemic stroke	479:499	acute ischemic stroke	479:499	Methods and Results: Patients with NVAF who experienced acute ischemic stroke or TIA of the middle cerebral artery territory and started rivaroxaban within 30 days after onset were enrolled and were followed up for 90 days.
34789635	6	50	theme	%	1249:1249	arg1	interval					1262:1269	95% confidence interval	1247:1269	95% confidence interval	1247:1269	Associations between bridging therapy and ischemic events or major bleeding were not statistically significant individually, but the association between bridging therapy and their composite was statistically significant (multivariable-adjusted hazard ratio, 1.80; 95% confidence interval, 1.01-3.29).
34789635	0	51	with	Therapy	9:15	arg1	Heparin					22:28	Heparin	22:28	Heparin	22:28	Bridging Therapy With Heparin Before Starting Rivaroxaban in Ischemic Stroke or Transient Ischemic Attack With Non-Valvular Atrial Fibrillation.
34789635	5	52	theme	unfractionated	904:917	arg1	heparin					943:949	unfractionated or low-molecular-weight heparin	904:949	heparin	943:949	Of 1,308 analyzed patients, 638 received bridging therapy with unfractionated or low-molecular-weight heparin with a median of 10,000 IU/day.
34789635	8	53	theme	ischemic	1628:1635	arg1	stroke					1637:1642	ischemic stroke	1628:1642	ischemic stroke	1628:1642	CONCLUSIONS The composite of ischemic events and major bleeding was more frequent in patients with NVAF who received bridging therapy with low-dose heparin than in those who started treatment directly with rivaroxaban after ischemic stroke or TIA.
34789635	6	54	theme	multivariable-adjusted	1204:1225	arg1	association					1116:1126	the association	1112:1126	the association between bridging therapy	1112:1151	Associations between bridging therapy and ischemic events or major bleeding were not statistically significant individually, but the association between bridging therapy and their composite was statistically significant (multivariable-adjusted hazard ratio, 1.80; 95% confidence interval, 1.01-3.29).
34789635	6	54	theme	multivariable-adjusted	1204:1225	arg1	significant					1191:1201	significant	1191:1201	significant	1191:1201	Associations between bridging therapy and ischemic events or major bleeding were not statistically significant individually, but the association between bridging therapy and their composite was statistically significant (multivariable-adjusted hazard ratio, 1.80; 95% confidence interval, 1.01-3.29).
34789635	6	54	theme	multivariable-adjusted	1204:1225	arg1	ratio					1234:1238	multivariable-adjusted hazard ratio	1204:1238	multivariable-adjusted hazard ratio	1204:1238	Associations between bridging therapy and ischemic events or major bleeding were not statistically significant individually, but the association between bridging therapy and their composite was statistically significant (multivariable-adjusted hazard ratio, 1.80; 95% confidence interval, 1.01-3.29).
34789635	6	55	theme	95	1247:1248	arg1	%					1249:1249	%	1249:1249	%	1249:1249	Associations between bridging therapy and ischemic events or major bleeding were not statistically significant individually, but the association between bridging therapy and their composite was statistically significant (multivariable-adjusted hazard ratio, 1.80; 95% confidence interval, 1.01-3.29).
34789635	8	56	theme	bridging	1521:1528	arg1	therapy					1530:1536	bridging therapy	1521:1536	bridging therapy with low-dose heparin	1521:1558	CONCLUSIONS The composite of ischemic events and major bleeding was more frequent in patients with NVAF who received bridging therapy with low-dose heparin than in those who started treatment directly with rivaroxaban after ischemic stroke or TIA.
34789635	5	57	theme	low-molecular-weight	922:941	arg1	heparin					943:949	unfractionated or low-molecular-weight heparin	904:949	heparin	943:949	Of 1,308 analyzed patients, 638 received bridging therapy with unfractionated or low-molecular-weight heparin with a median of 10,000 IU/day.
34789635	0	58	theme	Stroke	70:75	arg1	Attack					99:104	Ischemic Stroke or Transient Ischemic Attack	61:104	Ischemic Stroke or Transient Ischemic Attack	61:104	Bridging Therapy With Heparin Before Starting Rivaroxaban in Ischemic Stroke or Transient Ischemic Attack With Non-Valvular Atrial Fibrillation.
34789635	6	59	theme	1.80	1241:1244	arg1	association					1116:1126	the association	1112:1126	the association between bridging therapy	1112:1151	Associations between bridging therapy and ischemic events or major bleeding were not statistically significant individually, but the association between bridging therapy and their composite was statistically significant (multivariable-adjusted hazard ratio, 1.80; 95% confidence interval, 1.01-3.29).
34789635	6	59	theme	1.80	1241:1244	arg1	significant					1191:1201	significant	1191:1201	significant	1191:1201	Associations between bridging therapy and ischemic events or major bleeding were not statistically significant individually, but the association between bridging therapy and their composite was statistically significant (multivariable-adjusted hazard ratio, 1.80; 95% confidence interval, 1.01-3.29).
34789635	6	59	theme	1.80	1241:1244	arg1	ratio					1234:1238	multivariable-adjusted hazard ratio	1204:1238	multivariable-adjusted hazard ratio	1204:1238	Associations between bridging therapy and ischemic events or major bleeding were not statistically significant individually, but the association between bridging therapy and their composite was statistically significant (multivariable-adjusted hazard ratio, 1.80; 95% confidence interval, 1.01-3.29).
34789635	0	60	with	Rivaroxaban	46:56	arg1	Fibrillation					131:142	Non-Valvular Atrial Fibrillation	111:142	Non-Valvular Atrial Fibrillation	111:142	Bridging Therapy With Heparin Before Starting Rivaroxaban in Ischemic Stroke or Transient Ischemic Attack With Non-Valvular Atrial Fibrillation.
34789635	5	61	theme	analyzed	850:857	arg1	patients					859:866	1,308 analyzed patients	844:866	1,308 analyzed patients	844:866	Of 1,308 analyzed patients, 638 received bridging therapy with unfractionated or low-molecular-weight heparin with a median of 10,000 IU/day.
34789635	6	62	theme	ischemic	1025:1032	arg1	events					1034:1039	ischemic events	1025:1039	ischemic events	1025:1039	Associations between bridging therapy and ischemic events or major bleeding were not statistically significant individually, but the association between bridging therapy and their composite was statistically significant (multivariable-adjusted hazard ratio, 1.80; 95% confidence interval, 1.01-3.29).
34789635	0	63	theme	Ischemic	61:68	arg1	Attack					99:104	Ischemic Stroke or Transient Ischemic Attack	61:104	Ischemic Stroke or Transient Ischemic Attack	61:104	Bridging Therapy With Heparin Before Starting Rivaroxaban in Ischemic Stroke or Transient Ischemic Attack With Non-Valvular Atrial Fibrillation.
34789635	4	64	theme	ischemic	796:803	arg1	stroke					805:810	ischemic stroke	796:810	ischemic stroke	796:810	Ischemic events were defined as ischemic stroke, TIA, and systemic embolism.
34789635	4	64	theme	ischemic	796:803	arg1	events					773:778	Ischemic events	764:778	Ischemic events	764:778	Ischemic events were defined as ischemic stroke, TIA, and systemic embolism.
34789635	1	65	theme	present	160:166	arg1	study					182:186	The present observational study	156:186	The present observational study	156:186	BACKGROUND The present observational study aimed to clarify the association between bridging therapy with heparin before starting rivaroxaban and clinical outcomes after ischemic stroke or transient ischemic attack (TIA) in patients with non-valvular atrial fibrillation (NVAF).
34450255	6	0	contain	had	1049:1051	arg1	which					1043:1047	which	1043:1047	which	1043:1047	Specifically, miR-142a-3p promoted the growth of Lactobacillus reuteri, which had a high abundance in the feces of mice recovered from colitis, by regulating transcripts of polA and locus tag LREU_RS03575.
34450255	6	0	contain	had	1049:1051	arg2	abundance					1060:1068	a high abundance	1053:1068	a high abundance in the feces of mice recovered from colitis	1053:1112	Specifically, miR-142a-3p promoted the growth of Lactobacillus reuteri, which had a high abundance in the feces of mice recovered from colitis, by regulating transcripts of polA and locus tag LREU_RS03575.
34450255	3	1	theme	fecal	318:322	arg1	miRNAs					324:329	fecal miRNAs	318:329	fecal miRNAs in subjects with inflammatory bowel diseases	318:374	However, whether fecal miRNAs in subjects with inflammatory bowel diseases are involved in regulating microbiota composition and whether they have any beneficial effects remains unknown.
34450255	4	2	theme	microbiome	515:524	arg1	composition					526:536	fecal microbiome composition	509:536	fecal microbiome composition	509:536	Here, we studied the fecal microbiome composition and miRNA abundance in mice with dextran sulfate sodium (DSS)-induced colitis and mice at the recovery phase to explore different miRNAs expressed, their relations with microbial abundance, and their effects on colitis.
34450255	9	3	theme	preventive	1471:1480	arg1	effects					1482:1488	preventive effects	1471:1488	preventive effects against those diseases	1471:1511	We propose that the feces of subjects who have recovered from diseases might be enriched with miRNAs with preventive effects against those diseases.
34450255	3	4	theme	inflammatory	348:359	arg1	diseases					367:374	inflammatory bowel diseases	348:374	inflammatory bowel diseases	348:374	However, whether fecal miRNAs in subjects with inflammatory bowel diseases are involved in regulating microbiota composition and whether they have any beneficial effects remains unknown.
34450255	4	5	theme	fecal	509:513	arg1	composition					526:536	fecal microbiome composition	509:536	fecal microbiome composition	509:536	Here, we studied the fecal microbiome composition and miRNA abundance in mice with dextran sulfate sodium (DSS)-induced colitis and mice at the recovery phase to explore different miRNAs expressed, their relations with microbial abundance, and their effects on colitis.
34450255	8	6	theme	fecal	1317:1321	arg1	miR-142a-3p					1323:1333	fecal miR-142a-3p	1317:1333	fecal miR-142a-3p	1317:1333	These results highlight the role of fecal miR-142a-3p in the prevention of colitis.
34450255	9	7	with	miRNAs	1459:1464	arg1	effects					1482:1488	preventive effects	1471:1488	preventive effects against those diseases	1471:1511	We propose that the feces of subjects who have recovered from diseases might be enriched with miRNAs with preventive effects against those diseases.
34450255	2	8	theme	diseased	282:289	arg1	subjects					291:298	healthy and diseased subjects	270:298	healthy and diseased subjects	270:298	These miRNAs are differentially expressed in the feces of healthy and diseased subjects.
34450255	4	9	from	miRNAs	668:673	arg1	colitis					749:755	colitis	749:755	colitis	749:755	Here, we studied the fecal microbiome composition and miRNA abundance in mice with dextran sulfate sodium (DSS)-induced colitis and mice at the recovery phase to explore different miRNAs expressed, their relations with microbial abundance, and their effects on colitis.
34450255	3	10	theme	bowel	361:365	arg1	diseases					367:374	inflammatory bowel diseases	348:374	inflammatory bowel diseases	348:374	However, whether fecal miRNAs in subjects with inflammatory bowel diseases are involved in regulating microbiota composition and whether they have any beneficial effects remains unknown.
34450255	0	11	from	mice	66:69	arg1	miR-142a-3p					6:16	Fecal miR-142a-3p	0:16	Fecal miR-142a-3p from dextran sulfate sodium-challenge recovered mice	0:69	Fecal miR-142a-3p from dextran sulfate sodium-challenge recovered mice prevents colitis by promoting the growth of Lactobacillus reuteri.
34450255	6	12	theme	locus	1153:1157	arg1	LREU_RS03575					1163:1174	locus tag LREU_RS03575	1153:1174	locus tag LREU_RS03575	1153:1174	Specifically, miR-142a-3p promoted the growth of Lactobacillus reuteri, which had a high abundance in the feces of mice recovered from colitis, by regulating transcripts of polA and locus tag LREU_RS03575.
34450255	5	13	theme	microbiome-dependent	942:961	arg1	manner					963:968	a microbiome-dependent manner	940:968	a microbiome-dependent manner	940:968	We found that miR-142a-3p expression was significantly increased in the feces of mice recovered from colitis and that it could alleviate disease symptoms in mice treated with DSS in a microbiome-dependent manner.
34450255	9	14	theme	subjects	1394:1401	arg1	feces					1385:1389	the feces	1381:1389	the feces of subjects who have recovered from diseases	1381:1434	We propose that the feces of subjects who have recovered from diseases might be enriched with miRNAs with preventive effects against those diseases.
34450255	7	15	theme	metabolite	1214:1223	arg1	reuterin					1225:1232	its metabolite reuterin	1210:1232	its metabolite reuterin	1210:1232	Moreover, L. reuteri, as well as its metabolite reuterin, could alleviate DSS-induced disease symptoms.
34450255	6	16	from	abundance	1060:1068	arg1	feces					1077:1081	the feces	1073:1081	the feces of mice recovered from colitis	1073:1112	Specifically, miR-142a-3p promoted the growth of Lactobacillus reuteri, which had a high abundance in the feces of mice recovered from colitis, by regulating transcripts of polA and locus tag LREU_RS03575.
34450255	2	17	theme	healthy	270:276	arg1	subjects					291:298	healthy and diseased subjects	270:298	healthy and diseased subjects	270:298	These miRNAs are differentially expressed in the feces of healthy and diseased subjects.
34450255	6	18	theme	high	1055:1058	arg1	abundance					1060:1068	a high abundance	1053:1068	a high abundance in the feces of mice recovered from colitis	1053:1112	Specifically, miR-142a-3p promoted the growth of Lactobacillus reuteri, which had a high abundance in the feces of mice recovered from colitis, by regulating transcripts of polA and locus tag LREU_RS03575.
34450255	0	19	theme	recovered	56:64	arg1	mice					66:69	dextran sulfate sodium-challenge recovered mice	23:69	dextran sulfate sodium-challenge recovered mice	23:69	Fecal miR-142a-3p from dextran sulfate sodium-challenge recovered mice prevents colitis by promoting the growth of Lactobacillus reuteri.
34450255	6	20	dep	Lactobacillus	1020:1032	arg1	reuteri					1034:1040	Lactobacillus reuteri, which had a high abundance in the feces of mice recovered from colitis,	1020:1113	reuteri	1034:1040	Specifically, miR-142a-3p promoted the growth of Lactobacillus reuteri, which had a high abundance in the feces of mice recovered from colitis, by regulating transcripts of polA and locus tag LREU_RS03575.
34450255	6	20	dep	Lactobacillus	1020:1032	arg1	had					1049:1051	had	1049:1051	had a high abundance in the feces of mice recovered from colitis	1049:1112	Specifically, miR-142a-3p promoted the growth of Lactobacillus reuteri, which had a high abundance in the feces of mice recovered from colitis, by regulating transcripts of polA and locus tag LREU_RS03575.
34450255	4	21	from	abundance	548:556	arg1	mice					561:564	mice	561:564	mice with dextran sulfate sodium (DSS)-induced colitis	561:614	Here, we studied the fecal microbiome composition and miRNA abundance in mice with dextran sulfate sodium (DSS)-induced colitis and mice at the recovery phase to explore different miRNAs expressed, their relations with microbial abundance, and their effects on colitis.
34450255	4	21	from	abundance	548:556	arg1	mice					620:623	mice	620:623	mice at the recovery phase	620:645	Here, we studied the fecal microbiome composition and miRNA abundance in mice with dextran sulfate sodium (DSS)-induced colitis and mice at the recovery phase to explore different miRNAs expressed, their relations with microbial abundance, and their effects on colitis.
34450255	4	21	from	abundance	548:556	arg1	phase					641:645	the recovery phase	628:645	the recovery phase	628:645	Here, we studied the fecal microbiome composition and miRNA abundance in mice with dextran sulfate sodium (DSS)-induced colitis and mice at the recovery phase to explore different miRNAs expressed, their relations with microbial abundance, and their effects on colitis.
34450255	4	22	from	effects	738:744	arg1	colitis					749:755	colitis	749:755	colitis	749:755	Here, we studied the fecal microbiome composition and miRNA abundance in mice with dextran sulfate sodium (DSS)-induced colitis and mice at the recovery phase to explore different miRNAs expressed, their relations with microbial abundance, and their effects on colitis.
34450255	6	23	theme	polA	1144:1147	arg1	transcripts					1129:1139	transcripts	1129:1139	transcripts of polA and locus tag LREU_RS03575	1129:1174	Specifically, miR-142a-3p promoted the growth of Lactobacillus reuteri, which had a high abundance in the feces of mice recovered from colitis, by regulating transcripts of polA and locus tag LREU_RS03575.
34450255	4	24	with	miRNAs	668:673	arg1	abundance					717:725	microbial abundance	707:725	microbial abundance	707:725	Here, we studied the fecal microbiome composition and miRNA abundance in mice with dextran sulfate sodium (DSS)-induced colitis and mice at the recovery phase to explore different miRNAs expressed, their relations with microbial abundance, and their effects on colitis.
34450255	7	25	theme	disease	1263:1269	arg1	symptoms					1271:1278	DSS-induced disease symptoms	1251:1278	DSS-induced disease symptoms	1251:1278	Moreover, L. reuteri, as well as its metabolite reuterin, could alleviate DSS-induced disease symptoms.
34450255	4	26	from	phase	641:645	arg1	mice					561:564	mice	561:564	mice with dextran sulfate sodium (DSS)-induced colitis	561:614	Here, we studied the fecal microbiome composition and miRNA abundance in mice with dextran sulfate sodium (DSS)-induced colitis and mice at the recovery phase to explore different miRNAs expressed, their relations with microbial abundance, and their effects on colitis.
34450255	4	26	from	phase	641:645	arg1	composition					526:536	fecal microbiome composition	509:536	fecal microbiome composition	509:536	Here, we studied the fecal microbiome composition and miRNA abundance in mice with dextran sulfate sodium (DSS)-induced colitis and mice at the recovery phase to explore different miRNAs expressed, their relations with microbial abundance, and their effects on colitis.
34450255	4	26	from	phase	641:645	arg1	abundance					548:556	miRNA abundance	542:556	miRNA abundance	542:556	Here, we studied the fecal microbiome composition and miRNA abundance in mice with dextran sulfate sodium (DSS)-induced colitis and mice at the recovery phase to explore different miRNAs expressed, their relations with microbial abundance, and their effects on colitis.
34450255	4	26	from	phase	641:645	arg1	mice					620:623	mice	620:623	mice at the recovery phase	620:645	Here, we studied the fecal microbiome composition and miRNA abundance in mice with dextran sulfate sodium (DSS)-induced colitis and mice at the recovery phase to explore different miRNAs expressed, their relations with microbial abundance, and their effects on colitis.
34450255	4	27	theme	different	658:666	arg1	miRNAs					668:673	different miRNAs	658:673	different miRNAs expressed	658:683	Here, we studied the fecal microbiome composition and miRNA abundance in mice with dextran sulfate sodium (DSS)-induced colitis and mice at the recovery phase to explore different miRNAs expressed, their relations with microbial abundance, and their effects on colitis.
34450255	0	28	theme	Fecal	0:4	arg1	miR-142a-3p					6:16	Fecal miR-142a-3p	0:16	Fecal miR-142a-3p from dextran sulfate sodium-challenge recovered mice	0:69	Fecal miR-142a-3p from dextran sulfate sodium-challenge recovered mice prevents colitis by promoting the growth of Lactobacillus reuteri.
34450255	4	29	with	mice	620:623	arg1	colitis					608:614	dextran sulfate sodium (DSS)-induced colitis	571:614	dextran sulfate sodium (DSS)-induced colitis	571:614	Here, we studied the fecal microbiome composition and miRNA abundance in mice with dextran sulfate sodium (DSS)-induced colitis and mice at the recovery phase to explore different miRNAs expressed, their relations with microbial abundance, and their effects on colitis.
34450255	4	30	dep	composition	526:536	arg1	the					505:507	the	505:507	the	505:507	Here, we studied the fecal microbiome composition and miRNA abundance in mice with dextran sulfate sodium (DSS)-induced colitis and mice at the recovery phase to explore different miRNAs expressed, their relations with microbial abundance, and their effects on colitis.
34450255	0	31	theme	dextran	23:29	arg1	sulfate					31:37	dextran sulfate	23:37	dextran sulfate sodium-challenge recovered mice	23:69	Fecal miR-142a-3p from dextran sulfate sodium-challenge recovered mice prevents colitis by promoting the growth of Lactobacillus reuteri.
34450255	5	32	from	symptoms	903:910	arg1	mice					915:918	mice	915:918	mice treated with DSS	915:935	We found that miR-142a-3p expression was significantly increased in the feces of mice recovered from colitis and that it could alleviate disease symptoms in mice treated with DSS in a microbiome-dependent manner.
34450255	8	33	theme	colitis	1356:1362	arg1	prevention					1342:1351	the prevention	1338:1351	the prevention of colitis	1338:1362	These results highlight the role of fecal miR-142a-3p in the prevention of colitis.
34450255	4	34	with	mice	561:564	arg1	colitis					608:614	dextran sulfate sodium (DSS)-induced colitis	571:614	dextran sulfate sodium (DSS)-induced colitis	571:614	Here, we studied the fecal microbiome composition and miRNA abundance in mice with dextran sulfate sodium (DSS)-induced colitis and mice at the recovery phase to explore different miRNAs expressed, their relations with microbial abundance, and their effects on colitis.
34450255	4	35	with	relations	692:700	arg1	abundance					717:725	microbial abundance	707:725	microbial abundance	707:725	Here, we studied the fecal microbiome composition and miRNA abundance in mice with dextran sulfate sodium (DSS)-induced colitis and mice at the recovery phase to explore different miRNAs expressed, their relations with microbial abundance, and their effects on colitis.
34450255	0	36	theme	reuteri	129:135	arg1	growth					105:110	the growth	101:110	the growth of Lactobacillus reuteri	101:135	Fecal miR-142a-3p from dextran sulfate sodium-challenge recovered mice prevents colitis by promoting the growth of Lactobacillus reuteri.
34450255	2	37	theme	subjects	291:298	arg1	feces					261:265	the feces	257:265	the feces of healthy and diseased subjects	257:298	These miRNAs are differentially expressed in the feces of healthy and diseased subjects.
34450255	1	38	theme	gut	196:198	arg1	microbiota					200:209	the gut microbiota	192:209	the gut microbiota	192:209	Feces are enriched with microRNAs (miRNAs) that shape the gut microbiota.
34450255	0	39	theme	sodium-challenge	39:54	arg1	mice					66:69	dextran sulfate sodium-challenge recovered mice	23:69	dextran sulfate sodium-challenge recovered mice	23:69	Fecal miR-142a-3p from dextran sulfate sodium-challenge recovered mice prevents colitis by promoting the growth of Lactobacillus reuteri.
34450255	4	40	theme	recovery	632:639	arg1	phase					641:645	the recovery phase	628:645	the recovery phase	628:645	Here, we studied the fecal microbiome composition and miRNA abundance in mice with dextran sulfate sodium (DSS)-induced colitis and mice at the recovery phase to explore different miRNAs expressed, their relations with microbial abundance, and their effects on colitis.
34450255	3	41	with	subjects	334:341	arg1	diseases					367:374	inflammatory bowel diseases	348:374	inflammatory bowel diseases	348:374	However, whether fecal miRNAs in subjects with inflammatory bowel diseases are involved in regulating microbiota composition and whether they have any beneficial effects remains unknown.
34450255	3	42	theme	beneficial	452:461	arg1	effects					463:469	any beneficial effects	448:469	any beneficial effects	448:469	However, whether fecal miRNAs in subjects with inflammatory bowel diseases are involved in regulating microbiota composition and whether they have any beneficial effects remains unknown.
34450255	0	43	theme	sulfate	31:37	arg1	mice					66:69	dextran sulfate sodium-challenge recovered mice	23:69	dextran sulfate sodium-challenge recovered mice	23:69	Fecal miR-142a-3p from dextran sulfate sodium-challenge recovered mice prevents colitis by promoting the growth of Lactobacillus reuteri.
34450255	4	44	with	effects	738:744	arg1	abundance					717:725	microbial abundance	707:725	microbial abundance	707:725	Here, we studied the fecal microbiome composition and miRNA abundance in mice with dextran sulfate sodium (DSS)-induced colitis and mice at the recovery phase to explore different miRNAs expressed, their relations with microbial abundance, and their effects on colitis.
34450255	5	45	theme	mice	839:842	arg1	feces					830:834	the feces	826:834	the feces of mice recovered from colitis	826:865	We found that miR-142a-3p expression was significantly increased in the feces of mice recovered from colitis and that it could alleviate disease symptoms in mice treated with DSS in a microbiome-dependent manner.
34450255	5	46	theme	miR-142a-3p	772:782	arg1	expression					784:793	miR-142a-3p expression	772:793	miR-142a-3p expression	772:793	We found that miR-142a-3p expression was significantly increased in the feces of mice recovered from colitis and that it could alleviate disease symptoms in mice treated with DSS in a microbiome-dependent manner.
34450255	4	47	from	composition	526:536	arg1	mice					561:564	mice	561:564	mice with dextran sulfate sodium (DSS)-induced colitis	561:614	Here, we studied the fecal microbiome composition and miRNA abundance in mice with dextran sulfate sodium (DSS)-induced colitis and mice at the recovery phase to explore different miRNAs expressed, their relations with microbial abundance, and their effects on colitis.
34450255	4	47	from	composition	526:536	arg1	mice					620:623	mice	620:623	mice at the recovery phase	620:645	Here, we studied the fecal microbiome composition and miRNA abundance in mice with dextran sulfate sodium (DSS)-induced colitis and mice at the recovery phase to explore different miRNAs expressed, their relations with microbial abundance, and their effects on colitis.
34450255	4	47	from	composition	526:536	arg1	phase					641:645	the recovery phase	628:645	the recovery phase	628:645	Here, we studied the fecal microbiome composition and miRNA abundance in mice with dextran sulfate sodium (DSS)-induced colitis and mice at the recovery phase to explore different miRNAs expressed, their relations with microbial abundance, and their effects on colitis.
34450255	5	48	theme	disease	895:901	arg1	symptoms					903:910	disease symptoms	895:910	disease symptoms in mice treated with DSS	895:935	We found that miR-142a-3p expression was significantly increased in the feces of mice recovered from colitis and that it could alleviate disease symptoms in mice treated with DSS in a microbiome-dependent manner.
34450255	3	49	theme	microbiota	403:412	arg1	composition					414:424	microbiota composition	403:424	microbiota composition	403:424	However, whether fecal miRNAs in subjects with inflammatory bowel diseases are involved in regulating microbiota composition and whether they have any beneficial effects remains unknown.
34450255	7	50	theme	DSS-induced	1251:1261	arg1	symptoms					1271:1278	DSS-induced disease symptoms	1251:1278	DSS-induced disease symptoms	1251:1278	Moreover, L. reuteri, as well as its metabolite reuterin, could alleviate DSS-induced disease symptoms.
34450255	4	51	theme	miRNA	542:546	arg1	abundance					548:556	miRNA abundance	542:556	miRNA abundance	542:556	Here, we studied the fecal microbiome composition and miRNA abundance in mice with dextran sulfate sodium (DSS)-induced colitis and mice at the recovery phase to explore different miRNAs expressed, their relations with microbial abundance, and their effects on colitis.
34450255	8	52	from	role	1309:1312	arg1	prevention					1342:1351	the prevention	1338:1351	the prevention of colitis	1338:1362	These results highlight the role of fecal miR-142a-3p in the prevention of colitis.
34450255	4	53	theme	-induced	599:606	arg1	colitis					608:614	dextran sulfate sodium (DSS)-induced colitis	571:614	dextran sulfate sodium (DSS)-induced colitis	571:614	Here, we studied the fecal microbiome composition and miRNA abundance in mice with dextran sulfate sodium (DSS)-induced colitis and mice at the recovery phase to explore different miRNAs expressed, their relations with microbial abundance, and their effects on colitis.
34450255	8	54	theme	miR-142a-3p	1323:1333	arg1	role					1309:1312	the role	1305:1312	the role of fecal miR-142a-3p in the prevention of colitis	1305:1362	These results highlight the role of fecal miR-142a-3p in the prevention of colitis.
34450255	3	55	from	miRNAs	324:329	arg1	subjects					334:341	subjects	334:341	subjects with inflammatory bowel diseases	334:374	However, whether fecal miRNAs in subjects with inflammatory bowel diseases are involved in regulating microbiota composition and whether they have any beneficial effects remains unknown.
34450255	6	56	theme	tag	1159:1161	arg1	LREU_RS03575					1163:1174	locus tag LREU_RS03575	1153:1174	locus tag LREU_RS03575	1153:1174	Specifically, miR-142a-3p promoted the growth of Lactobacillus reuteri, which had a high abundance in the feces of mice recovered from colitis, by regulating transcripts of polA and locus tag LREU_RS03575.
34450255	4	57	theme	microbial	707:715	arg1	abundance					717:725	microbial abundance	707:725	microbial abundance	707:725	Here, we studied the fecal microbiome composition and miRNA abundance in mice with dextran sulfate sodium (DSS)-induced colitis and mice at the recovery phase to explore different miRNAs expressed, their relations with microbial abundance, and their effects on colitis.
34450255	3	58	contain	have	443:446	arg2	effects					463:469	any beneficial effects	448:469	any beneficial effects	448:469	However, whether fecal miRNAs in subjects with inflammatory bowel diseases are involved in regulating microbiota composition and whether they have any beneficial effects remains unknown.
34450255	3	58	contain	have	443:446	arg1	they					438:441	they	438:441	they	438:441	However, whether fecal miRNAs in subjects with inflammatory bowel diseases are involved in regulating microbiota composition and whether they have any beneficial effects remains unknown.
34450255	6	59	theme	mice	1086:1089	arg1	feces					1077:1081	the feces	1073:1081	the feces of mice recovered from colitis	1073:1112	Specifically, miR-142a-3p promoted the growth of Lactobacillus reuteri, which had a high abundance in the feces of mice recovered from colitis, by regulating transcripts of polA and locus tag LREU_RS03575.
34450255	4	60	from	relations	692:700	arg1	colitis					749:755	colitis	749:755	colitis	749:755	Here, we studied the fecal microbiome composition and miRNA abundance in mice with dextran sulfate sodium (DSS)-induced colitis and mice at the recovery phase to explore different miRNAs expressed, their relations with microbial abundance, and their effects on colitis.
34450255	6	61	theme	LREU_RS03575	1163:1174	arg1	transcripts					1129:1139	transcripts	1129:1139	transcripts of polA and locus tag LREU_RS03575	1129:1174	Specifically, miR-142a-3p promoted the growth of Lactobacillus reuteri, which had a high abundance in the feces of mice recovered from colitis, by regulating transcripts of polA and locus tag LREU_RS03575.
34450255	6	62	theme	Lactobacillus	1020:1032	arg1	growth					1010:1015	the growth	1006:1015	the growth of Lactobacillus reuteri, which had a high abundance in the feces of mice recovered from colitis,	1006:1113	Specifically, miR-142a-3p promoted the growth of Lactobacillus reuteri, which had a high abundance in the feces of mice recovered from colitis, by regulating transcripts of polA and locus tag LREU_RS03575.
32464443	10	0	theme	hot	1460:1462	arg1	spots					1464:1468	hot spots	1460:1468	hot spots generated on the AgNPs-PAAm/starch hydrogel substrate, which leads to potential use for organic pollutant detection	1460:1584	The spectra with enhanced intensities were possible due to a large number of hot spots generated on the AgNPs-PAAm/starch hydrogel substrate, which leads to potential use for organic pollutant detection.
32464443	1	1	theme	surface-enhanced	304:319	arg1	SERS					339:342	SERS	339:342	SERS	339:342	Herein is presented a simple and sensible method to determine organic pollutants in water, based on the utilization of silver nanoparticles (AgNPs) loaded in Polyacrylamide (PAAm)/starch hybrid hydrogels combined with surface-enhanced Raman scattering (SERS) spectroscopy.
32464443	1	1	theme	surface-enhanced	304:319	arg1	scattering					327:336	surface-enhanced Raman scattering	304:336	surface-enhanced Raman scattering (SERS) spectroscopy	304:356	Herein is presented a simple and sensible method to determine organic pollutants in water, based on the utilization of silver nanoparticles (AgNPs) loaded in Polyacrylamide (PAAm)/starch hybrid hydrogels combined with surface-enhanced Raman scattering (SERS) spectroscopy.
32464443	9	2	theme	AgNPs-PAAm/starch	1304:1320	arg1	hydrogels					1322:1330	AgNPs-PAAm/starch hydrogels	1304:1330	AgNPs-PAAm/starch hydrogels used for each detection analysis	1304:1363	The average mass of AgNPs-PAAm/starch hydrogels used for each detection analysis was around 10 mg.
32464443	8	3	theme	phenol	1253:1258	arg1	detection					1234:1242	detection	1234:1242	detection (LOD) of phenol in water of 1 × 10-8 M	1234:1281	The SERS substrate based on the AgNPs-PAAm/starch exhibited reproducibility, stability, and limit of detection (LOD) of phenol in water of 1 × 10-8 M.
32464443	8	3	theme	phenol	1253:1258	arg1	LOD					1245:1247	LOD	1245:1247	LOD	1245:1247	The SERS substrate based on the AgNPs-PAAm/starch exhibited reproducibility, stability, and limit of detection (LOD) of phenol in water of 1 × 10-8 M.
32464443	2	4	theme	X-ray	454:458	arg1	XRD					473:475	XRD	473:475	XRD	473:475	The materials were characterized by swelling degree studies, UV-Visible spectroscopy (UV-Vis), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
32464443	2	4	theme	X-ray	454:458	arg1	diffraction					460:470	X-ray diffraction	454:470	X-ray diffraction (XRD)	454:476	The materials were characterized by swelling degree studies, UV-Visible spectroscopy (UV-Vis), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
32464443	1	5	theme	Raman	321:325	arg1	SERS					339:342	SERS	339:342	SERS	339:342	Herein is presented a simple and sensible method to determine organic pollutants in water, based on the utilization of silver nanoparticles (AgNPs) loaded in Polyacrylamide (PAAm)/starch hybrid hydrogels combined with surface-enhanced Raman scattering (SERS) spectroscopy.
32464443	1	5	theme	Raman	321:325	arg1	scattering					327:336	surface-enhanced Raman scattering	304:336	surface-enhanced Raman scattering (SERS) spectroscopy	304:356	Herein is presented a simple and sensible method to determine organic pollutants in water, based on the utilization of silver nanoparticles (AgNPs) loaded in Polyacrylamide (PAAm)/starch hybrid hydrogels combined with surface-enhanced Raman scattering (SERS) spectroscopy.
32464443	7	6	theme	nanoparticles	1054:1066	arg1	quantity					1042:1049	quantity	1042:1049	quantity	1042:1049	The average size of the AgNPs was smaller than 100 nm and the size and quantity of nanoparticles were influenced by the molar composition of the hydrogel matrix.
32464443	7	6	theme	nanoparticles	1054:1066	arg1	size					1033:1036	size	1033:1036	size	1033:1036	The average size of the AgNPs was smaller than 100 nm and the size and quantity of nanoparticles were influenced by the molar composition of the hydrogel matrix.
32464443	1	7	theme	organic	148:154	arg1	pollutants					156:165	organic pollutants	148:165	organic pollutants	148:165	Herein is presented a simple and sensible method to determine organic pollutants in water, based on the utilization of silver nanoparticles (AgNPs) loaded in Polyacrylamide (PAAm)/starch hybrid hydrogels combined with surface-enhanced Raman scattering (SERS) spectroscopy.
32464443	2	8	theme	electron	491:498	arg1	SEM					512:514	SEM	512:514	SEM	512:514	The materials were characterized by swelling degree studies, UV-Visible spectroscopy (UV-Vis), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
32464443	2	8	theme	electron	491:498	arg1	microscopy					500:509	scanning electron microscopy	482:509	scanning electron microscopy (SEM)	482:515	The materials were characterized by swelling degree studies, UV-Visible spectroscopy (UV-Vis), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
32464443	1	9	theme	scattering	327:336	arg1	spectroscopy					345:356	surface-enhanced Raman scattering (SERS) spectroscopy	304:356	surface-enhanced Raman scattering (SERS) spectroscopy	304:356	Herein is presented a simple and sensible method to determine organic pollutants in water, based on the utilization of silver nanoparticles (AgNPs) loaded in Polyacrylamide (PAAm)/starch hybrid hydrogels combined with surface-enhanced Raman scattering (SERS) spectroscopy.
32464443	8	10	from	detection	1234:1242	arg1	water					1263:1267	water	1263:1267	water of 1 × 10-8 M	1263:1281	The SERS substrate based on the AgNPs-PAAm/starch exhibited reproducibility, stability, and limit of detection (LOD) of phenol in water of 1 × 10-8 M.
32464443	10	11	theme	hydrogel	1505:1512	arg1	substrate					1514:1522	the AgNPs-PAAm/starch hydrogel substrate	1483:1522	the AgNPs-PAAm/starch hydrogel substrate	1483:1522	The spectra with enhanced intensities were possible due to a large number of hot spots generated on the AgNPs-PAAm/starch hydrogel substrate, which leads to potential use for organic pollutant detection.
32464443	9	12	theme	detection	1346:1354	arg1	analysis					1356:1363	each detection analysis	1341:1363	each detection analysis	1341:1363	The average mass of AgNPs-PAAm/starch hydrogels used for each detection analysis was around 10 mg.
32464443	2	13	theme	scanning	482:489	arg1	SEM					512:514	SEM	512:514	SEM	512:514	The materials were characterized by swelling degree studies, UV-Visible spectroscopy (UV-Vis), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
32464443	2	13	theme	scanning	482:489	arg1	microscopy					500:509	scanning electron microscopy	482:509	scanning electron microscopy (SEM)	482:515	The materials were characterized by swelling degree studies, UV-Visible spectroscopy (UV-Vis), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
32464443	10	14	theme	potential	1540:1548	arg1	use					1550:1552	potential use	1540:1552	potential use for organic pollutant detection	1540:1584	The spectra with enhanced intensities were possible due to a large number of hot spots generated on the AgNPs-PAAm/starch hydrogel substrate, which leads to potential use for organic pollutant detection.
32464443	4	15	dep	agent	712:716	arg1	N					719:719	N	719:719	N	719:719	The most promising results were attributed to lower concentrations of starch and crosslink agent (N,N'-methylenebisacrylamide - MBA).
32464443	4	15	dep	agent	712:716	arg1	N'-methylenebisacrylamide					721:745	N'-methylenebisacrylamide	721:745	N'-methylenebisacrylamide	721:745	The most promising results were attributed to lower concentrations of starch and crosslink agent (N,N'-methylenebisacrylamide - MBA).
32464443	4	16	theme	lower	667:671	arg1	concentrations					673:686	lower concentrations	667:686	lower concentrations of starch and crosslink agent (N,N'-methylenebisacrylamide - MBA)	667:752	The most promising results were attributed to lower concentrations of starch and crosslink agent (N,N'-methylenebisacrylamide - MBA).
32464443	5	17	theme	surface	862:868	arg1	SPR					889:891	SPR	889:891	SPR	889:891	Spectroscopic analysis confirmed the formation of AgNPs, by noticing the peak at around 420 nm, due to its surface plasmon resonance (SPR) effect.
32464443	5	17	theme	surface	862:868	arg1	resonance					878:886	its surface plasmon resonance	858:886	its surface plasmon resonance (SPR) effect	858:899	Spectroscopic analysis confirmed the formation of AgNPs, by noticing the peak at around 420 nm, due to its surface plasmon resonance (SPR) effect.
32464443	10	18	theme	enhanced	1400:1407	arg1	intensities					1409:1419	enhanced intensities	1400:1419	enhanced intensities	1400:1419	The spectra with enhanced intensities were possible due to a large number of hot spots generated on the AgNPs-PAAm/starch hydrogel substrate, which leads to potential use for organic pollutant detection.
32464443	8	19	from	limit	1225:1229	arg1	water					1263:1267	water	1263:1267	water of 1 × 10-8 M	1263:1281	The SERS substrate based on the AgNPs-PAAm/starch exhibited reproducibility, stability, and limit of detection (LOD) of phenol in water of 1 × 10-8 M.
32464443	8	20	theme	1 × 10-8 M	1272:1281	arg1	water					1263:1267	water	1263:1267	water of 1 × 10-8 M	1263:1281	The SERS substrate based on the AgNPs-PAAm/starch exhibited reproducibility, stability, and limit of detection (LOD) of phenol in water of 1 × 10-8 M.
32464443	5	21	theme	resonance	878:886	arg1	effect					894:899	its surface plasmon resonance (SPR) effect	858:899	its surface plasmon resonance (SPR) effect	858:899	Spectroscopic analysis confirmed the formation of AgNPs, by noticing the peak at around 420 nm, due to its surface plasmon resonance (SPR) effect.
32464443	4	22	theme	promising	630:638	arg1	results					640:646	The most promising results	621:646	The most promising results	621:646	The most promising results were attributed to lower concentrations of starch and crosslink agent (N,N'-methylenebisacrylamide - MBA).
32464443	7	23	theme	matrix	1125:1130	arg1	composition					1097:1107	the molar composition	1087:1107	the molar composition of the hydrogel matrix	1087:1130	The average size of the AgNPs was smaller than 100 nm and the size and quantity of nanoparticles were influenced by the molar composition of the hydrogel matrix.
32464443	11	24	from	substrate	1661:1669	arg1	analyzes					1680:1687	other analyzes	1674:1687	other analyzes	1674:1687	In addition, there is also the possibility of reusing the hydrogel matrix substrate in other analyzes.
32464443	0	25	theme	ECO-FRIENDLY	0:11	arg1	hydrogels					20:28	ECO-FRIENDLY hybrid hydrogels	0:28	ECO-FRIENDLY hybrid hydrogels for detection of phenolic RESIDUES in water using SERS.	0:84	ECO-FRIENDLY hybrid hydrogels for detection of phenolic RESIDUES in water using SERS.
32464443	4	26	theme	most	625:628	arg1	results					640:646	The most promising results	621:646	The most promising results	621:646	The most promising results were attributed to lower concentrations of starch and crosslink agent (N,N'-methylenebisacrylamide - MBA).
32464443	0	27	theme	hybrid	13:18	arg1	hydrogels					20:28	ECO-FRIENDLY hybrid hydrogels	0:28	ECO-FRIENDLY hybrid hydrogels for detection of phenolic RESIDUES in water using SERS.	0:84	ECO-FRIENDLY hybrid hydrogels for detection of phenolic RESIDUES in water using SERS.
32464443	0	28	from	detection	34:42	arg1	water					68:72	water	68:72	water using SERS	68:83	ECO-FRIENDLY hybrid hydrogels for detection of phenolic RESIDUES in water using SERS.
32464443	2	29	dep	spectroscopy	431:442	arg1	UV-Vis					445:450	UV-Vis	445:450	UV-Vis	445:450	The materials were characterized by swelling degree studies, UV-Visible spectroscopy (UV-Vis), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
32464443	8	30	from	based	1152:1156	arg1	AgNPs-PAAm/starch					1165:1181	the AgNPs-PAAm/starch	1161:1181	the AgNPs-PAAm/starch	1161:1181	The SERS substrate based on the AgNPs-PAAm/starch exhibited reproducibility, stability, and limit of detection (LOD) of phenol in water of 1 × 10-8 M.
32464443	10	31	theme	pollutant	1566:1574	arg1	detection					1576:1584	organic pollutant detection	1558:1584	organic pollutant detection	1558:1584	The spectra with enhanced intensities were possible due to a large number of hot spots generated on the AgNPs-PAAm/starch hydrogel substrate, which leads to potential use for organic pollutant detection.
32464443	2	32	theme	UV-Visible	420:429	arg1	spectroscopy					431:442	UV-Visible spectroscopy	420:442	UV-Visible spectroscopy (UV-Vis)	420:451	The materials were characterized by swelling degree studies, UV-Visible spectroscopy (UV-Vis), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
32464443	11	33	theme	other	1674:1678	arg1	analyzes					1680:1687	other analyzes	1674:1687	other analyzes	1674:1687	In addition, there is also the possibility of reusing the hydrogel matrix substrate in other analyzes.
32464443	4	34	theme	agent	712:716	arg1	concentrations					673:686	lower concentrations	667:686	lower concentrations of starch and crosslink agent (N,N'-methylenebisacrylamide - MBA)	667:752	The most promising results were attributed to lower concentrations of starch and crosslink agent (N,N'-methylenebisacrylamide - MBA).
32464443	10	35	with	spectra	1387:1393	arg1	intensities					1409:1419	enhanced intensities	1400:1419	enhanced intensities	1400:1419	The spectra with enhanced intensities were possible due to a large number of hot spots generated on the AgNPs-PAAm/starch hydrogel substrate, which leads to potential use for organic pollutant detection.
32464443	10	36	theme	organic	1558:1564	arg1	detection					1576:1584	organic pollutant detection	1558:1584	organic pollutant detection	1558:1584	The spectra with enhanced intensities were possible due to a large number of hot spots generated on the AgNPs-PAAm/starch hydrogel substrate, which leads to potential use for organic pollutant detection.
32464443	7	37	theme	molar	1091:1095	arg1	composition					1097:1107	the molar composition	1087:1107	the molar composition of the hydrogel matrix	1087:1130	The average size of the AgNPs was smaller than 100 nm and the size and quantity of nanoparticles were influenced by the molar composition of the hydrogel matrix.
32464443	2	38	theme	degree	404:409	arg1	studies					411:417	degree studies	404:417	degree studies	404:417	The materials were characterized by swelling degree studies, UV-Visible spectroscopy (UV-Vis), X-ray diffraction (XRD) and scanning electron microscopy (SEM).
32464443	5	39	theme	AgNPs	805:809	arg1	formation					792:800	the formation	788:800	the formation of AgNPs	788:809	Spectroscopic analysis confirmed the formation of AgNPs, by noticing the peak at around 420 nm, due to its surface plasmon resonance (SPR) effect.
32464443	7	40	dep	size	1033:1036	arg1	the					1029:1031	the	1029:1031	the	1029:1031	The average size of the AgNPs was smaller than 100 nm and the size and quantity of nanoparticles were influenced by the molar composition of the hydrogel matrix.
32464443	3	41	theme	PAAm/starch	518:528	arg1	hydrogels					530:538	PAAm/starch hydrogels	518:538	PAAm/starch hydrogels	518:538	PAAm/starch hydrogels showed variable swelling capacity, according to the synthetic molar composition.
32464443	8	42	theme	substrate	1142:1150	arg1	based					1152:1156	The SERS substrate based	1133:1156	The SERS substrate based on the AgNPs-PAAm/starch	1133:1181	The SERS substrate based on the AgNPs-PAAm/starch exhibited reproducibility, stability, and limit of detection (LOD) of phenol in water of 1 × 10-8 M.
32464443	4	43	dep	N	719:719	arg1	MBA					749:751	MBA	749:751	MBA	749:751	The most promising results were attributed to lower concentrations of starch and crosslink agent (N,N'-methylenebisacrylamide - MBA).
32464443	7	44	theme	AgNPs	995:999	arg1	smaller					1005:1011	smaller	1005:1011	smaller	1005:1011	The average size of the AgNPs was smaller than 100 nm and the size and quantity of nanoparticles were influenced by the molar composition of the hydrogel matrix.
32464443	7	44	theme	AgNPs	995:999	arg1	size					983:986	The average size	971:986	The average size of the AgNPs	971:999	The average size of the AgNPs was smaller than 100 nm and the size and quantity of nanoparticles were influenced by the molar composition of the hydrogel matrix.
32464443	5	45	theme	Spectroscopic	755:767	arg1	analysis					769:776	Spectroscopic analysis	755:776	Spectroscopic analysis	755:776	Spectroscopic analysis confirmed the formation of AgNPs, by noticing the peak at around 420 nm, due to its surface plasmon resonance (SPR) effect.
32464443	1	46	theme	/starch	265:271	arg1	hydrogels					280:288	Polyacrylamide (PAAm)/starch hybrid hydrogels	244:288	Polyacrylamide (PAAm)/starch hybrid hydrogels combined with surface-enhanced Raman scattering (SERS) spectroscopy	244:356	Herein is presented a simple and sensible method to determine organic pollutants in water, based on the utilization of silver nanoparticles (AgNPs) loaded in Polyacrylamide (PAAm)/starch hybrid hydrogels combined with surface-enhanced Raman scattering (SERS) spectroscopy.
32464443	8	47	theme	SERS	1137:1140	arg1	based					1152:1156	The SERS substrate based	1133:1156	The SERS substrate based on the AgNPs-PAAm/starch	1133:1181	The SERS substrate based on the AgNPs-PAAm/starch exhibited reproducibility, stability, and limit of detection (LOD) of phenol in water of 1 × 10-8 M.
32464443	3	48	theme	molar	602:606	arg1	composition					608:618	the synthetic molar composition	588:618	the synthetic molar composition	588:618	PAAm/starch hydrogels showed variable swelling capacity, according to the synthetic molar composition.
32464443	5	49	theme	plasmon	870:876	arg1	SPR					889:891	SPR	889:891	SPR	889:891	Spectroscopic analysis confirmed the formation of AgNPs, by noticing the peak at around 420 nm, due to its surface plasmon resonance (SPR) effect.
32464443	5	49	theme	plasmon	870:876	arg1	resonance					878:886	its surface plasmon resonance	858:886	its surface plasmon resonance (SPR) effect	858:899	Spectroscopic analysis confirmed the formation of AgNPs, by noticing the peak at around 420 nm, due to its surface plasmon resonance (SPR) effect.
32464443	8	50	from	reproducibility	1193:1207	arg1	water					1263:1267	water	1263:1267	water of 1 × 10-8 M	1263:1281	The SERS substrate based on the AgNPs-PAAm/starch exhibited reproducibility, stability, and limit of detection (LOD) of phenol in water of 1 × 10-8 M.
32464443	8	51	theme	detection	1234:1242	arg1	stability					1210:1218	stability	1210:1218	stability	1210:1218	The SERS substrate based on the AgNPs-PAAm/starch exhibited reproducibility, stability, and limit of detection (LOD) of phenol in water of 1 × 10-8 M.
32464443	8	51	theme	detection	1234:1242	arg1	limit					1225:1229	limit	1225:1229	limit	1225:1229	The SERS substrate based on the AgNPs-PAAm/starch exhibited reproducibility, stability, and limit of detection (LOD) of phenol in water of 1 × 10-8 M.
32464443	8	51	theme	detection	1234:1242	arg1	reproducibility					1193:1207	reproducibility	1193:1207	reproducibility	1193:1207	The SERS substrate based on the AgNPs-PAAm/starch exhibited reproducibility, stability, and limit of detection (LOD) of phenol in water of 1 × 10-8 M.
32464443	0	52	theme	RESIDUES	56:63	arg1	detection					34:42	detection	34:42	detection of phenolic RESIDUES in water using SERS	34:83	ECO-FRIENDLY hybrid hydrogels for detection of phenolic RESIDUES in water using SERS.
32464443	1	53	theme	hybrid	273:278	arg1	hydrogels					280:288	Polyacrylamide (PAAm)/starch hybrid hydrogels	244:288	Polyacrylamide (PAAm)/starch hybrid hydrogels combined with surface-enhanced Raman scattering (SERS) spectroscopy	244:356	Herein is presented a simple and sensible method to determine organic pollutants in water, based on the utilization of silver nanoparticles (AgNPs) loaded in Polyacrylamide (PAAm)/starch hybrid hydrogels combined with surface-enhanced Raman scattering (SERS) spectroscopy.
32464443	3	54	theme	variable	547:554	arg1	capacity					565:572	variable swelling capacity	547:572	variable swelling capacity	547:572	PAAm/starch hydrogels showed variable swelling capacity, according to the synthetic molar composition.
32464443	11	55	theme	matrix	1654:1659	arg1	substrate					1661:1669	the hydrogel matrix substrate	1641:1669	the hydrogel matrix substrate in other analyzes	1641:1687	In addition, there is also the possibility of reusing the hydrogel matrix substrate in other analyzes.
32464443	4	56	theme	starch	691:696	arg1	concentrations					673:686	lower concentrations	667:686	lower concentrations of starch and crosslink agent (N,N'-methylenebisacrylamide - MBA)	667:752	The most promising results were attributed to lower concentrations of starch and crosslink agent (N,N'-methylenebisacrylamide - MBA).
32464443	0	57	theme	phenolic	47:54	arg1	RESIDUES					56:63	phenolic RESIDUES	47:63	phenolic RESIDUES	47:63	ECO-FRIENDLY hybrid hydrogels for detection of phenolic RESIDUES in water using SERS.
32464443	9	58	theme	average	1288:1294	arg1	mass					1296:1299	The average mass	1284:1299	The average mass of AgNPs-PAAm/starch hydrogels used for each detection analysis	1284:1363	The average mass of AgNPs-PAAm/starch hydrogels used for each detection analysis was around 10 mg.
32464443	10	59	theme	large	1444:1448	arg1	number					1450:1455	a large number	1442:1455	a large number of hot spots generated on the AgNPs-PAAm/starch hydrogel substrate, which leads to potential use for organic pollutant detection	1442:1584	The spectra with enhanced intensities were possible due to a large number of hot spots generated on the AgNPs-PAAm/starch hydrogel substrate, which leads to potential use for organic pollutant detection.
32464443	3	60	theme	swelling	556:563	arg1	capacity					565:572	variable swelling capacity	547:572	variable swelling capacity	547:572	PAAm/starch hydrogels showed variable swelling capacity, according to the synthetic molar composition.
32464443	1	61	theme	simple	108:113	arg1	method					128:133	a simple and sensible method	106:133	a simple and sensible method to determine organic pollutants in water, based on the utilization of silver nanoparticles (AgNPs) loaded in Polyacrylamide (PAAm)/starch hybrid hydrogels combined with surface-enhanced Raman scattering (SERS) spectroscopy	106:356	Herein is presented a simple and sensible method to determine organic pollutants in water, based on the utilization of silver nanoparticles (AgNPs) loaded in Polyacrylamide (PAAm)/starch hybrid hydrogels combined with surface-enhanced Raman scattering (SERS) spectroscopy.
32464443	7	62	theme	average	975:981	arg1	smaller					1005:1011	smaller	1005:1011	smaller	1005:1011	The average size of the AgNPs was smaller than 100 nm and the size and quantity of nanoparticles were influenced by the molar composition of the hydrogel matrix.
32464443	7	62	theme	average	975:981	arg1	size					983:986	The average size	971:986	The average size of the AgNPs	971:999	The average size of the AgNPs was smaller than 100 nm and the size and quantity of nanoparticles were influenced by the molar composition of the hydrogel matrix.
32464443	11	63	theme	hydrogel	1645:1652	arg1	substrate					1661:1669	the hydrogel matrix substrate	1641:1669	the hydrogel matrix substrate in other analyzes	1641:1687	In addition, there is also the possibility of reusing the hydrogel matrix substrate in other analyzes.
32464443	4	64	theme	crosslink	702:710	arg1	agent					712:716	crosslink agent	702:716	crosslink agent (N,N'-methylenebisacrylamide - MBA)	702:752	The most promising results were attributed to lower concentrations of starch and crosslink agent (N,N'-methylenebisacrylamide - MBA).
32464443	8	65	from	stability	1210:1218	arg1	water					1263:1267	water	1263:1267	water of 1 × 10-8 M	1263:1281	The SERS substrate based on the AgNPs-PAAm/starch exhibited reproducibility, stability, and limit of detection (LOD) of phenol in water of 1 × 10-8 M.
32464443	3	66	theme	synthetic	592:600	arg1	composition					608:618	the synthetic molar composition	588:618	the synthetic molar composition	588:618	PAAm/starch hydrogels showed variable swelling capacity, according to the synthetic molar composition.
32464443	9	67	theme	hydrogels	1322:1330	arg1	mass					1296:1299	The average mass	1284:1299	The average mass of AgNPs-PAAm/starch hydrogels used for each detection analysis	1284:1363	The average mass of AgNPs-PAAm/starch hydrogels used for each detection analysis was around 10 mg.
32464443	1	68	theme	sensible	119:126	arg1	method					128:133	a simple and sensible method	106:133	a simple and sensible method to determine organic pollutants in water, based on the utilization of silver nanoparticles (AgNPs) loaded in Polyacrylamide (PAAm)/starch hybrid hydrogels combined with surface-enhanced Raman scattering (SERS) spectroscopy	106:356	Herein is presented a simple and sensible method to determine organic pollutants in water, based on the utilization of silver nanoparticles (AgNPs) loaded in Polyacrylamide (PAAm)/starch hybrid hydrogels combined with surface-enhanced Raman scattering (SERS) spectroscopy.
32464443	8	69	from	water	1263:1267	arg1	stability					1210:1218	stability	1210:1218	stability	1210:1218	The SERS substrate based on the AgNPs-PAAm/starch exhibited reproducibility, stability, and limit of detection (LOD) of phenol in water of 1 × 10-8 M.
32464443	8	69	from	water	1263:1267	arg1	limit					1225:1229	limit	1225:1229	limit	1225:1229	The SERS substrate based on the AgNPs-PAAm/starch exhibited reproducibility, stability, and limit of detection (LOD) of phenol in water of 1 × 10-8 M.
32464443	8	69	from	water	1263:1267	arg1	reproducibility					1193:1207	reproducibility	1193:1207	reproducibility	1193:1207	The SERS substrate based on the AgNPs-PAAm/starch exhibited reproducibility, stability, and limit of detection (LOD) of phenol in water of 1 × 10-8 M.
32464443	1	70	theme	silver	205:210	arg1	nanoparticles					212:224	silver nanoparticles	205:224	silver nanoparticles (AgNPs) loaded in Polyacrylamide (PAAm)/starch hybrid hydrogels combined with surface-enhanced Raman scattering (SERS) spectroscopy	205:356	Herein is presented a simple and sensible method to determine organic pollutants in water, based on the utilization of silver nanoparticles (AgNPs) loaded in Polyacrylamide (PAAm)/starch hybrid hydrogels combined with surface-enhanced Raman scattering (SERS) spectroscopy.
32464443	1	70	theme	silver	205:210	arg1	AgNPs					227:231	AgNPs	227:231	AgNPs	227:231	Herein is presented a simple and sensible method to determine organic pollutants in water, based on the utilization of silver nanoparticles (AgNPs) loaded in Polyacrylamide (PAAm)/starch hybrid hydrogels combined with surface-enhanced Raman scattering (SERS) spectroscopy.
32464443	1	71	theme	nanoparticles	212:224	arg1	utilization					190:200	the utilization	186:200	the utilization of silver nanoparticles (AgNPs) loaded in Polyacrylamide (PAAm)/starch hybrid hydrogels combined with surface-enhanced Raman scattering (SERS) spectroscopy	186:356	Herein is presented a simple and sensible method to determine organic pollutants in water, based on the utilization of silver nanoparticles (AgNPs) loaded in Polyacrylamide (PAAm)/starch hybrid hydrogels combined with surface-enhanced Raman scattering (SERS) spectroscopy.
32464443	10	72	theme	spots	1464:1468	arg1	number					1450:1455	a large number	1442:1455	a large number of hot spots generated on the AgNPs-PAAm/starch hydrogel substrate, which leads to potential use for organic pollutant detection	1442:1584	The spectra with enhanced intensities were possible due to a large number of hot spots generated on the AgNPs-PAAm/starch hydrogel substrate, which leads to potential use for organic pollutant detection.
32464443	7	73	theme	hydrogel	1116:1123	arg1	matrix					1125:1130	the hydrogel matrix	1112:1130	the hydrogel matrix	1112:1130	The average size of the AgNPs was smaller than 100 nm and the size and quantity of nanoparticles were influenced by the molar composition of the hydrogel matrix.
32464443	1	74	from	pollutants	156:165	arg1	water					170:174	water	170:174	water	170:174	Herein is presented a simple and sensible method to determine organic pollutants in water, based on the utilization of silver nanoparticles (AgNPs) loaded in Polyacrylamide (PAAm)/starch hybrid hydrogels combined with surface-enhanced Raman scattering (SERS) spectroscopy.
32464443	10	75	theme	AgNPs-PAAm/starch	1487:1503	arg1	substrate					1514:1522	the AgNPs-PAAm/starch hydrogel substrate	1483:1522	the AgNPs-PAAm/starch hydrogel substrate	1483:1522	The spectra with enhanced intensities were possible due to a large number of hot spots generated on the AgNPs-PAAm/starch hydrogel substrate, which leads to potential use for organic pollutant detection.
32442573	0	0	theme	2-naphthol	88:97	arg1	removal					77:83	the removal	73:83	the removal of 2-naphthol from aqueous solution and their kinetic studies	73:145	Magnetic graphene/chitosan nanocomposite: A promising nano-adsorbent for the removal of 2-naphthol from aqueous solution and their kinetic studies.
32442573	6	1	from	capacity	1033:1040	arg1	pH 2					1062:1065	pH 2	1062:1065	pH 2	1062:1065	The batch mode adsorption studies demonstrates that MGCH based adsorbent showed almost 99.8% adsorption of 2-naphthol with a maximum adsorption capacity of 169.49 mg g-1 at pH 2.
32442573	8	2	theme	phenolic	1302:1309	arg1	pollutants					1311:1320	phenolic pollutants	1302:1320	phenolic pollutants from contaminated wastewater	1302:1349	This work proves that MGCH nanocomposite can be used as high-performance adsorbent for removing of phenolic pollutants from contaminated wastewater.
32442573	6	3	theme	maximum	1014:1020	arg1	capacity					1033:1040	a maximum adsorption capacity	1012:1040	a maximum adsorption capacity of 169.49 mg g-1 at pH 2	1012:1065	The batch mode adsorption studies demonstrates that MGCH based adsorbent showed almost 99.8% adsorption of 2-naphthol with a maximum adsorption capacity of 169.49 mg g-1 at pH 2.
32442573	8	4	theme	contaminated	1327:1338	arg1	wastewater					1340:1349	contaminated wastewater	1327:1349	contaminated wastewater	1327:1349	This work proves that MGCH nanocomposite can be used as high-performance adsorbent for removing of phenolic pollutants from contaminated wastewater.
32442573	6	5	with	adsorption	982:991	arg1	capacity					1033:1040	a maximum adsorption capacity	1012:1040	a maximum adsorption capacity of 169.49 mg g-1 at pH 2	1012:1065	The batch mode adsorption studies demonstrates that MGCH based adsorbent showed almost 99.8% adsorption of 2-naphthol with a maximum adsorption capacity of 169.49 mg g-1 at pH 2.
32442573	1	6	from	solution	331:338	arg1	removal					296:302	the removal	292:302	the removal of 2-naphthol from aqueous solution	292:338	In this study, magnetic/graphene/chitosan nanocomposite (MGCH) is prepared through facile solvothermal process and employed as an adsorbent for the removal of 2-naphthol from aqueous solution.
32442573	4	7	theme	saturation	703:712	arg1	magnetization					714:726	higher saturation magnetization	696:726	higher saturation magnetization of 46.5 emu g-1 and lower coercivity (Hc) of 50 Oe	696:777	VSM result of MGCH composite exhibited higher saturation magnetization of 46.5 emu g-1 and lower coercivity (Hc) of 50 Oe.
32442573	0	8	theme	aqueous	104:110	arg1	solution					112:119	aqueous solution	104:119	aqueous solution	104:119	Magnetic graphene/chitosan nanocomposite: A promising nano-adsorbent for the removal of 2-naphthol from aqueous solution and their kinetic studies.
32442573	5	9	contain	possesses	812:820	arg2	response					829:836	response	829:836	response required for the separation from aqueous solution	829:886	This result discloses that MGCH possesses enough response required for the separation from aqueous solution.
32442573	5	9	contain	possesses	812:820	arg1	MGCH					807:810	MGCH	807:810	MGCH	807:810	This result discloses that MGCH possesses enough response required for the separation from aqueous solution.
32442573	8	10	used	used	1251:1254	arg2	nanocomposite					1230:1242	MGCH nanocomposite	1225:1242	MGCH nanocomposite	1225:1242	This work proves that MGCH nanocomposite can be used as high-performance adsorbent for removing of phenolic pollutants from contaminated wastewater.
32442573	8	10	used	used	1251:1254	arg2	adsorbent					1276:1284	adsorbent	1276:1284	adsorbent	1276:1284	This work proves that MGCH nanocomposite can be used as high-performance adsorbent for removing of phenolic pollutants from contaminated wastewater.
32442573	6	11	theme	adsorption	904:913	arg1	studies					915:921	adsorption studies	904:921	The batch mode adsorption studies	889:921	The batch mode adsorption studies demonstrates that MGCH based adsorbent showed almost 99.8% adsorption of 2-naphthol with a maximum adsorption capacity of 169.49 mg g-1 at pH 2.
32442573	6	12	theme	adsorbent	952:960	arg1	based					946:950	MGCH based adsorbent	941:960	MGCH based adsorbent	941:960	The batch mode adsorption studies demonstrates that MGCH based adsorbent showed almost 99.8% adsorption of 2-naphthol with a maximum adsorption capacity of 169.49 mg g-1 at pH 2.
32442573	3	13	theme	spherical-like	542:555	arg1	nanoparticles					563:575	spherical-like Fe3O4 nanoparticles	542:575	spherical-like Fe3O4 nanoparticles	542:575	The FESEM and EDS analysis reveals that the high density of spherical-like Fe3O4 nanoparticles and chitosan are successfully assembled on the surfaces of the graphene sheets.
32442573	6	14	theme	2-naphthol	996:1005	arg1	adsorption					982:991	almost 99.8% adsorption	969:991	almost 99.8% adsorption of 2-naphthol with a maximum adsorption capacity of 169.49 mg g-1 at pH 2	969:1065	The batch mode adsorption studies demonstrates that MGCH based adsorbent showed almost 99.8% adsorption of 2-naphthol with a maximum adsorption capacity of 169.49 mg g-1 at pH 2.
32442573	7	15	theme	system	1195:1200	arg1	nature					1170:1175	the nature	1166:1175	the nature of the adsorption system	1166:1200	Moreover, the kinetic studies of the samples are performed by fitting adsorption models to ensure the nature of the adsorption system.
32442573	4	16	theme	46.5 emu g-1	731:742	arg1	magnetization					714:726	higher saturation magnetization	696:726	higher saturation magnetization of 46.5 emu g-1 and lower coercivity (Hc) of 50 Oe	696:777	VSM result of MGCH composite exhibited higher saturation magnetization of 46.5 emu g-1 and lower coercivity (Hc) of 50 Oe.
32442573	7	17	theme	samples	1105:1111	arg1	studies					1090:1096	the kinetic studies	1078:1096	the kinetic studies of the samples	1078:1111	Moreover, the kinetic studies of the samples are performed by fitting adsorption models to ensure the nature of the adsorption system.
32442573	3	18	theme	high	526:529	arg1	density					531:537	the high density	522:537	the high density of spherical-like Fe3O4 nanoparticles	522:575	The FESEM and EDS analysis reveals that the high density of spherical-like Fe3O4 nanoparticles and chitosan are successfully assembled on the surfaces of the graphene sheets.
32442573	1	19	theme	magnetic/graphene/chitosan	163:188	arg1	MGCH					205:208	MGCH	205:208	MGCH	205:208	In this study, magnetic/graphene/chitosan nanocomposite (MGCH) is prepared through facile solvothermal process and employed as an adsorbent for the removal of 2-naphthol from aqueous solution.
32442573	1	19	theme	magnetic/graphene/chitosan	163:188	arg1	nanocomposite					190:202	magnetic/graphene/chitosan nanocomposite	163:202	magnetic/graphene/chitosan nanocomposite (MGCH)	163:209	In this study, magnetic/graphene/chitosan nanocomposite (MGCH) is prepared through facile solvothermal process and employed as an adsorbent for the removal of 2-naphthol from aqueous solution.
32442573	1	19	theme	magnetic/graphene/chitosan	163:188	arg1	adsorbent					278:286	adsorbent	278:286	adsorbent	278:286	In this study, magnetic/graphene/chitosan nanocomposite (MGCH) is prepared through facile solvothermal process and employed as an adsorbent for the removal of 2-naphthol from aqueous solution.
32442573	6	20	theme	batch	893:897	arg1	mode					899:902	The batch mode adsorption studies	889:921	The batch mode adsorption studies	889:921	The batch mode adsorption studies demonstrates that MGCH based adsorbent showed almost 99.8% adsorption of 2-naphthol with a maximum adsorption capacity of 169.49 mg g-1 at pH 2.
32442573	6	21	from	pH 2	1062:1065	arg1	capacity					1033:1040	a maximum adsorption capacity	1012:1040	a maximum adsorption capacity of 169.49 mg g-1 at pH 2	1012:1065	The batch mode adsorption studies demonstrates that MGCH based adsorbent showed almost 99.8% adsorption of 2-naphthol with a maximum adsorption capacity of 169.49 mg g-1 at pH 2.
32442573	6	21	from	pH 2	1062:1065	arg1	169.49 mg g-1					1045:1057	169.49 mg g-1	1045:1057	169.49 mg g-1 at pH 2	1045:1065	The batch mode adsorption studies demonstrates that MGCH based adsorbent showed almost 99.8% adsorption of 2-naphthol with a maximum adsorption capacity of 169.49 mg g-1 at pH 2.
32442573	1	22	theme	solvothermal	238:249	arg1	process					251:257	facile solvothermal process	231:257	facile solvothermal process	231:257	In this study, magnetic/graphene/chitosan nanocomposite (MGCH) is prepared through facile solvothermal process and employed as an adsorbent for the removal of 2-naphthol from aqueous solution.
32442573	2	23	theme	FTIR	402:405	arg1	results					377:383	The physico-chemical characteristic results	341:383	The physico-chemical characteristic results of FESEM, Raman, FTIR, XRD and VSM	341:418	The physico-chemical characteristic results of FESEM, Raman, FTIR, XRD and VSM confirms that the MGCH nanocomposite is effectively prepared.
32442573	4	24	theme	composite	676:684	arg1	result					661:666	VSM result	657:666	VSM result of MGCH composite	657:684	VSM result of MGCH composite exhibited higher saturation magnetization of 46.5 emu g-1 and lower coercivity (Hc) of 50 Oe.
32442573	0	25	theme	Magnetic	0:7	arg1	graphene/chitosan					9:25	Magnetic graphene/chitosan	0:25	Magnetic graphene/chitosan	0:25	Magnetic graphene/chitosan nanocomposite: A promising nano-adsorbent for the removal of 2-naphthol from aqueous solution and their kinetic studies.
32442573	8	26	from	wastewater	1340:1349	arg1	pollutants					1311:1320	phenolic pollutants	1302:1320	phenolic pollutants from contaminated wastewater	1302:1349	This work proves that MGCH nanocomposite can be used as high-performance adsorbent for removing of phenolic pollutants from contaminated wastewater.
32442573	4	27	theme	coercivity	754:763	arg1	magnetization					714:726	higher saturation magnetization	696:726	higher saturation magnetization of 46.5 emu g-1 and lower coercivity (Hc) of 50 Oe	696:777	VSM result of MGCH composite exhibited higher saturation magnetization of 46.5 emu g-1 and lower coercivity (Hc) of 50 Oe.
32442573	2	28	theme	Raman	395:399	arg1	results					377:383	The physico-chemical characteristic results	341:383	The physico-chemical characteristic results of FESEM, Raman, FTIR, XRD and VSM	341:418	The physico-chemical characteristic results of FESEM, Raman, FTIR, XRD and VSM confirms that the MGCH nanocomposite is effectively prepared.
32442573	0	29	theme	kinetic	131:137	arg1	studies					139:145	their kinetic studies	125:145	their kinetic studies	125:145	Magnetic graphene/chitosan nanocomposite: A promising nano-adsorbent for the removal of 2-naphthol from aqueous solution and their kinetic studies.
32442573	3	30	theme	Fe3O4	557:561	arg1	nanoparticles					563:575	spherical-like Fe3O4 nanoparticles	542:575	spherical-like Fe3O4 nanoparticles	542:575	The FESEM and EDS analysis reveals that the high density of spherical-like Fe3O4 nanoparticles and chitosan are successfully assembled on the surfaces of the graphene sheets.
32442573	2	31	theme	MGCH	438:441	arg1	nanocomposite					443:455	the MGCH nanocomposite	434:455	the MGCH nanocomposite	434:455	The physico-chemical characteristic results of FESEM, Raman, FTIR, XRD and VSM confirms that the MGCH nanocomposite is effectively prepared.
32442573	3	32	theme	nanoparticles	563:575	arg1	density					531:537	the high density	522:537	the high density of spherical-like Fe3O4 nanoparticles	522:575	The FESEM and EDS analysis reveals that the high density of spherical-like Fe3O4 nanoparticles and chitosan are successfully assembled on the surfaces of the graphene sheets.
32442573	3	32	theme	nanoparticles	563:575	arg1	chitosan					581:588	chitosan	581:588	chitosan	581:588	The FESEM and EDS analysis reveals that the high density of spherical-like Fe3O4 nanoparticles and chitosan are successfully assembled on the surfaces of the graphene sheets.
32442573	2	33	theme	VSM	416:418	arg1	results					377:383	The physico-chemical characteristic results	341:383	The physico-chemical characteristic results of FESEM, Raman, FTIR, XRD and VSM	341:418	The physico-chemical characteristic results of FESEM, Raman, FTIR, XRD and VSM confirms that the MGCH nanocomposite is effectively prepared.
32442573	0	34	theme	promising	44:52	arg1	nano-adsorbent					54:67	A promising nano-adsorbent	42:67	A promising nano-adsorbent for the removal of 2-naphthol from aqueous solution and their kinetic studies	42:145	Magnetic graphene/chitosan nanocomposite: A promising nano-adsorbent for the removal of 2-naphthol from aqueous solution and their kinetic studies.
32442573	3	35	theme	EDS	496:498	arg1	analysis					500:507	The FESEM and EDS analysis	482:507	analysis	500:507	The FESEM and EDS analysis reveals that the high density of spherical-like Fe3O4 nanoparticles and chitosan are successfully assembled on the surfaces of the graphene sheets.
32442573	7	36	theme	adsorption	1184:1193	arg1	system					1195:1200	the adsorption system	1180:1200	the adsorption system	1180:1200	Moreover, the kinetic studies of the samples are performed by fitting adsorption models to ensure the nature of the adsorption system.
32442573	4	37	theme	MGCH	671:674	arg1	composite					676:684	MGCH composite	671:684	MGCH composite	671:684	VSM result of MGCH composite exhibited higher saturation magnetization of 46.5 emu g-1 and lower coercivity (Hc) of 50 Oe.
32442573	4	38	theme	VSM	657:659	arg1	result					661:666	VSM result	657:666	VSM result of MGCH composite	657:684	VSM result of MGCH composite exhibited higher saturation magnetization of 46.5 emu g-1 and lower coercivity (Hc) of 50 Oe.
32442573	6	39	theme	169.49 mg g-1	1045:1057	arg1	capacity					1033:1040	a maximum adsorption capacity	1012:1040	a maximum adsorption capacity of 169.49 mg g-1 at pH 2	1012:1065	The batch mode adsorption studies demonstrates that MGCH based adsorbent showed almost 99.8% adsorption of 2-naphthol with a maximum adsorption capacity of 169.49 mg g-1 at pH 2.
32442573	1	40	theme	2-naphthol	307:316	arg1	removal					296:302	the removal	292:302	the removal of 2-naphthol from aqueous solution	292:338	In this study, magnetic/graphene/chitosan nanocomposite (MGCH) is prepared through facile solvothermal process and employed as an adsorbent for the removal of 2-naphthol from aqueous solution.
32442573	3	41	theme	graphene	640:647	arg1	sheets					649:654	the graphene sheets	636:654	the graphene sheets	636:654	The FESEM and EDS analysis reveals that the high density of spherical-like Fe3O4 nanoparticles and chitosan are successfully assembled on the surfaces of the graphene sheets.
32442573	2	42	theme	XRD	408:410	arg1	results					377:383	The physico-chemical characteristic results	341:383	The physico-chemical characteristic results of FESEM, Raman, FTIR, XRD and VSM	341:418	The physico-chemical characteristic results of FESEM, Raman, FTIR, XRD and VSM confirms that the MGCH nanocomposite is effectively prepared.
32442573	7	43	theme	adsorption	1138:1147	arg1	models					1149:1154	adsorption models	1138:1154	adsorption models	1138:1154	Moreover, the kinetic studies of the samples are performed by fitting adsorption models to ensure the nature of the adsorption system.
32442573	3	44	theme	sheets	649:654	arg1	surfaces					624:631	the surfaces	620:631	the surfaces of the graphene sheets	620:654	The FESEM and EDS analysis reveals that the high density of spherical-like Fe3O4 nanoparticles and chitosan are successfully assembled on the surfaces of the graphene sheets.
32442573	2	45	theme	characteristic	362:375	arg1	results					377:383	The physico-chemical characteristic results	341:383	The physico-chemical characteristic results of FESEM, Raman, FTIR, XRD and VSM	341:418	The physico-chemical characteristic results of FESEM, Raman, FTIR, XRD and VSM confirms that the MGCH nanocomposite is effectively prepared.
32442573	8	46	theme	MGCH	1225:1228	arg1	adsorbent					1276:1284	adsorbent	1276:1284	adsorbent	1276:1284	This work proves that MGCH nanocomposite can be used as high-performance adsorbent for removing of phenolic pollutants from contaminated wastewater.
32442573	8	46	theme	MGCH	1225:1228	arg1	nanocomposite					1230:1242	MGCH nanocomposite	1225:1242	MGCH nanocomposite	1225:1242	This work proves that MGCH nanocomposite can be used as high-performance adsorbent for removing of phenolic pollutants from contaminated wastewater.
32442573	1	47	theme	aqueous	323:329	arg1	solution					331:338	aqueous solution	323:338	aqueous solution	323:338	In this study, magnetic/graphene/chitosan nanocomposite (MGCH) is prepared through facile solvothermal process and employed as an adsorbent for the removal of 2-naphthol from aqueous solution.
32442573	7	48	theme	kinetic	1082:1088	arg1	studies					1090:1096	the kinetic studies	1078:1096	the kinetic studies of the samples	1078:1111	Moreover, the kinetic studies of the samples are performed by fitting adsorption models to ensure the nature of the adsorption system.
32442573	6	49	dep	%	980:980	arg1	99.8					976:979	99.8	976:979	99.8	976:979	The batch mode adsorption studies demonstrates that MGCH based adsorbent showed almost 99.8% adsorption of 2-naphthol with a maximum adsorption capacity of 169.49 mg g-1 at pH 2.
32442573	2	50	theme	physico-chemical	345:360	arg1	results					377:383	The physico-chemical characteristic results	341:383	The physico-chemical characteristic results of FESEM, Raman, FTIR, XRD and VSM	341:418	The physico-chemical characteristic results of FESEM, Raman, FTIR, XRD and VSM confirms that the MGCH nanocomposite is effectively prepared.
32442573	0	51	from	solution	112:119	arg1	removal					77:83	the removal	73:83	the removal of 2-naphthol from aqueous solution and their kinetic studies	73:145	Magnetic graphene/chitosan nanocomposite: A promising nano-adsorbent for the removal of 2-naphthol from aqueous solution and their kinetic studies.
32442573	4	52	theme	lower	748:752	arg1	Hc					766:767	Hc	766:767	Hc	766:767	VSM result of MGCH composite exhibited higher saturation magnetization of 46.5 emu g-1 and lower coercivity (Hc) of 50 Oe.
32442573	4	52	theme	lower	748:752	arg1	coercivity					754:763	lower coercivity	748:763	lower coercivity (Hc)	748:768	VSM result of MGCH composite exhibited higher saturation magnetization of 46.5 emu g-1 and lower coercivity (Hc) of 50 Oe.
32442573	1	53	theme	facile	231:236	arg1	process					251:257	facile solvothermal process	231:257	facile solvothermal process	231:257	In this study, magnetic/graphene/chitosan nanocomposite (MGCH) is prepared through facile solvothermal process and employed as an adsorbent for the removal of 2-naphthol from aqueous solution.
32442573	4	54	theme	Oe	776:777	arg1	Hc					766:767	Hc	766:767	Hc	766:767	VSM result of MGCH composite exhibited higher saturation magnetization of 46.5 emu g-1 and lower coercivity (Hc) of 50 Oe.
32442573	4	54	theme	Oe	776:777	arg1	46.5 emu g-1					731:742	46.5 emu g-1	731:742	46.5 emu g-1	731:742	VSM result of MGCH composite exhibited higher saturation magnetization of 46.5 emu g-1 and lower coercivity (Hc) of 50 Oe.
32442573	4	54	theme	Oe	776:777	arg1	coercivity					754:763	lower coercivity	748:763	lower coercivity (Hc)	748:768	VSM result of MGCH composite exhibited higher saturation magnetization of 46.5 emu g-1 and lower coercivity (Hc) of 50 Oe.
32442573	5	55	from	solution	879:886	arg1	separation					855:864	the separation	851:864	the separation from aqueous solution	851:886	This result discloses that MGCH possesses enough response required for the separation from aqueous solution.
32442573	3	56	theme	FESEM	486:490	arg1	analysis					500:507	The FESEM and EDS analysis	482:507	analysis	500:507	The FESEM and EDS analysis reveals that the high density of spherical-like Fe3O4 nanoparticles and chitosan are successfully assembled on the surfaces of the graphene sheets.
32442573	0	57	from	studies	139:145	arg1	removal					77:83	the removal	73:83	the removal of 2-naphthol from aqueous solution and their kinetic studies	73:145	Magnetic graphene/chitosan nanocomposite: A promising nano-adsorbent for the removal of 2-naphthol from aqueous solution and their kinetic studies.
32442573	6	58	dep	adsorption	982:991	arg1	%					980:980	%	980:980	%	980:980	The batch mode adsorption studies demonstrates that MGCH based adsorbent showed almost 99.8% adsorption of 2-naphthol with a maximum adsorption capacity of 169.49 mg g-1 at pH 2.
32442573	4	59	theme	higher	696:701	arg1	magnetization					714:726	higher saturation magnetization	696:726	higher saturation magnetization of 46.5 emu g-1 and lower coercivity (Hc) of 50 Oe	696:777	VSM result of MGCH composite exhibited higher saturation magnetization of 46.5 emu g-1 and lower coercivity (Hc) of 50 Oe.
32442573	5	60	theme	aqueous	871:877	arg1	solution					879:886	aqueous solution	871:886	aqueous solution	871:886	This result discloses that MGCH possesses enough response required for the separation from aqueous solution.
32442573	6	61	theme	MGCH	941:944	arg1	based					946:950	MGCH based adsorbent	941:960	MGCH based adsorbent	941:960	The batch mode adsorption studies demonstrates that MGCH based adsorbent showed almost 99.8% adsorption of 2-naphthol with a maximum adsorption capacity of 169.49 mg g-1 at pH 2.
32442573	6	62	dep	mode	899:902	arg1	studies					915:921	adsorption studies	904:921	The batch mode adsorption studies	889:921	The batch mode adsorption studies demonstrates that MGCH based adsorbent showed almost 99.8% adsorption of 2-naphthol with a maximum adsorption capacity of 169.49 mg g-1 at pH 2.
32442573	2	63	theme	FESEM	388:392	arg1	results					377:383	The physico-chemical characteristic results	341:383	The physico-chemical characteristic results of FESEM, Raman, FTIR, XRD and VSM	341:418	The physico-chemical characteristic results of FESEM, Raman, FTIR, XRD and VSM confirms that the MGCH nanocomposite is effectively prepared.
32442573	6	64	theme	adsorption	1022:1031	arg1	capacity					1033:1040	a maximum adsorption capacity	1012:1040	a maximum adsorption capacity of 169.49 mg g-1 at pH 2	1012:1065	The batch mode adsorption studies demonstrates that MGCH based adsorbent showed almost 99.8% adsorption of 2-naphthol with a maximum adsorption capacity of 169.49 mg g-1 at pH 2.
32569317	7	0	theme	fungi	1356:1360	arg1	composition					1328:1338	biochemical composition	1316:1338	biochemical composition of Mucoromycota fungi and biomass yield	1316:1378	FTIR analysis of biochemical composition of Mucoromycota fungi and biomass yield showed that variation in inorganic phosphorus had higher effect when inorganic nitrogen source-ammonium sulphate-was used.
32569317	8	1	theme	Mucor	1729:1733	arg1	circinelloides					1735:1748	(2) Mucor circinelloides	1725:1748	(2) Mucor circinelloides	1725:1748	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	8	2	theme	inorganic	2290:2298	arg1	phosphorus					2300:2309	inorganic phosphorus	2290:2309	inorganic phosphorus	2290:2309	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	8	3	theme	Rhizopus	1975:1982	arg1	stolonifer					1984:1993	Rhizopus stolonifer	1975:1993	Rhizopus stolonifer	1975:1993	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	8	4	theme	Lichtheimia	1810:1820	arg1	chitin/chitosan					1847:1861	Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan	1791:1861	Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides	1791:1953	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	6	5	theme	inorganic	1147:1155	arg1	phosphorus					1157:1166	inorganic phosphorus	1147:1166	inorganic phosphorus	1147:1166	The influence of two types of nitrogen sources and different levels of inorganic phosphorus on the co-production of lipids, chitin/chitosan and polyphosphates for nine different oleaginous Mucoromycota fungi was evaluated.
32569317	8	6	theme	overproduced	1834:1845	arg1	chitin/chitosan					1847:1861	Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan	1791:1861	Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides	1791:1953	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	7	7	theme	nitrogen	1459:1466	arg1	sulphate-was					1484:1495	inorganic nitrogen source-ammonium sulphate-was	1449:1495	inorganic nitrogen source-ammonium sulphate-was	1449:1495	FTIR analysis of biochemical composition of Mucoromycota fungi and biomass yield showed that variation in inorganic phosphorus had higher effect when inorganic nitrogen source-ammonium sulphate-was used.
32569317	4	8	theme	FTIR	913:916	arg1	spectroscopy					918:929	FTIR spectroscopy	913:929	FTIR spectroscopy	913:929	To develop such biotechnological strategy, we applied a high throughput approach consisting of micro-titre cultivation and FTIR spectroscopy.
32569317	6	9	theme	oleaginous	1254:1263	arg1	fungi					1278:1282	nine different oleaginous Mucoromycota fungi	1239:1282	nine different oleaginous Mucoromycota fungi	1239:1282	The influence of two types of nitrogen sources and different levels of inorganic phosphorus on the co-production of lipids, chitin/chitosan and polyphosphates for nine different oleaginous Mucoromycota fungi was evaluated.
32569317	9	10	theme	several	2542:2548	arg1	fungi					2574:2578	several oleaginous Mucoromycota fungi	2542:2578	several oleaginous Mucoromycota fungi forming lipids, chitin/chitosan and polyphosphates in a single cultivation process	2542:2661	FTIR spectroscopy revealed the co-production potential of several oleaginous Mucoromycota fungi forming lipids, chitin/chitosan and polyphosphates in a single cultivation process.
32569317	9	11	from	lipids	2588:2593	arg1	process					2655:2661	a single cultivation process	2634:2661	a single cultivation process	2634:2661	FTIR spectroscopy revealed the co-production potential of several oleaginous Mucoromycota fungi forming lipids, chitin/chitosan and polyphosphates in a single cultivation process.
32569317	9	12	theme	Mucoromycota	2561:2572	arg1	fungi					2574:2578	several oleaginous Mucoromycota fungi	2542:2578	several oleaginous Mucoromycota fungi forming lipids, chitin/chitosan and polyphosphates in a single cultivation process	2542:2661	FTIR spectroscopy revealed the co-production potential of several oleaginous Mucoromycota fungi forming lipids, chitin/chitosan and polyphosphates in a single cultivation process.
32569317	8	13	theme	low	1873:1875	arg1	pH					1877:1878	the low pH	1869:1878	the low pH caused by the limitation of inorganic phosphorus	1869:1927	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	8	14	dep	biomass	2136:2142	arg1	4					2129:2129	4	2129:2129	4	2129:2129	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	8	15	theme	double	1563:1568	arg1	yield					1578:1582	almost double biomass yield	1556:1582	almost double biomass yield	1556:1582	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	0	16	from	co-production	83:95	arg1	fungi					150:154	oleaginous Mucoromycota fungi	126:154	oleaginous Mucoromycota fungi	126:154	Microcultivation and FTIR spectroscopy-based screening revealed a nutrient-induced co-production of high-value metabolites in oleaginous Mucoromycota fungi.
32569317	8	17	theme	same	2359:2362	arg1	amount					2364:2369	the same amount	2355:2369	the same amount of inorganic phosphorus combined with yeast extract	2355:2421	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	8	17	theme	same	2359:2362	arg1	phosphorus					2384:2393	inorganic phosphorus	2374:2393	inorganic phosphorus combined with yeast extract	2374:2421	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	8	18	dep	Absidia	1791:1797	arg1	glauca					1799:1804	glauca	1799:1804	glauca	1799:1804	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	3	19	theme	concept	645:651	arg1	development					616:626	the development	612:626	the development of co-production concept	612:651	Therefore, the development of co-production concept is highly desired since more than one valuable product could be produced at the time and the process has a potentially higher viability.
32569317	1	20	theme	Mucoromycota	157:168	arg1	fungi					170:174	Mucoromycota fungi	157:174	Mucoromycota fungi	157:174	Mucoromycota fungi possess a versatile metabolism and can utilize various substrates for production of industrially important products, such as lipids, chitin/chitosan, polyphosphates, pigments, alcohols and organic acids.
32569317	5	21	theme	biomass	1009:1015	arg1	fingerprinting					977:990	single-step biochemical fingerprinting	953:990	single-step biochemical fingerprinting of either fungal biomass or growth media	953:1031	This approach allows single-step biochemical fingerprinting of either fungal biomass or growth media without tedious extraction of metabolites.
32569317	8	22	dep	vinacea	1540:1546	arg1	1					1526:1526	1	1526:1526	1	1526:1526	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	7	23	theme	source-ammonium	1468:1482	arg1	sulphate-was					1484:1495	inorganic nitrogen source-ammonium sulphate-was	1449:1495	inorganic nitrogen source-ammonium sulphate-was	1449:1495	FTIR analysis of biochemical composition of Mucoromycota fungi and biomass yield showed that variation in inorganic phosphorus had higher effect when inorganic nitrogen source-ammonium sulphate-was used.
32569317	7	24	theme	Mucoromycota	1343:1354	arg1	fungi					1356:1360	Mucoromycota fungi	1343:1360	Mucoromycota fungi	1343:1360	FTIR analysis of biochemical composition of Mucoromycota fungi and biomass yield showed that variation in inorganic phosphorus had higher effect when inorganic nitrogen source-ammonium sulphate-was used.
32569317	8	25	theme	phosphorus	1918:1927	arg1	limitation					1894:1903	the limitation	1890:1903	the limitation of inorganic phosphorus	1890:1927	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	5	26	theme	single-step	953:963	arg1	fingerprinting					977:990	single-step biochemical fingerprinting	953:990	single-step biochemical fingerprinting of either fungal biomass or growth media	953:1031	This approach allows single-step biochemical fingerprinting of either fungal biomass or growth media without tedious extraction of metabolites.
32569317	2	27	theme	high	542:545	arg1	costs					558:562	the high production costs	538:562	the high production costs compared to the final product value	538:598	However, as far as commercialisation is concerned, establishing industrial biotechnological processes based on Mucoromycota fungi is still challenging due to the high production costs compared to the final product value.
32569317	8	28	theme	yeast	1615:1619	arg1	source					1650:1655	nitrogen source	1641:1655	nitrogen source	1641:1655	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	8	28	theme	yeast	1615:1619	arg1	extract					1621:1627	yeast extract	1615:1627	yeast extract	1615:1627	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	0	29	theme	high-value	100:109	arg1	metabolites					111:121	high-value metabolites	100:121	high-value metabolites	100:121	Microcultivation and FTIR spectroscopy-based screening revealed a nutrient-induced co-production of high-value metabolites in oleaginous Mucoromycota fungi.
32569317	9	30	theme	single	2636:2641	arg1	process					2655:2661	a single cultivation process	2634:2661	a single cultivation process	2634:2661	FTIR spectroscopy revealed the co-production potential of several oleaginous Mucoromycota fungi forming lipids, chitin/chitosan and polyphosphates in a single cultivation process.
32569317	2	31	theme	final	580:584	arg1	value					594:598	the final product value	576:598	the final product value	576:598	However, as far as commercialisation is concerned, establishing industrial biotechnological processes based on Mucoromycota fungi is still challenging due to the high production costs compared to the final product value.
32569317	4	32	theme	biotechnological	806:821	arg1	strategy					823:830	such biotechnological strategy	801:830	such biotechnological strategy	801:830	To develop such biotechnological strategy, we applied a high throughput approach consisting of micro-titre cultivation and FTIR spectroscopy.
32569317	8	33	theme	inorganic	2097:2105	arg1	phosphorus					2107:2116	inorganic phosphorus	2097:2116	inorganic phosphorus	2097:2116	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	9	34	from	polyphosphates	2616:2629	arg1	process					2655:2661	a single cultivation process	2634:2661	a single cultivation process	2634:2661	FTIR spectroscopy revealed the co-production potential of several oleaginous Mucoromycota fungi forming lipids, chitin/chitosan and polyphosphates in a single cultivation process.
32569317	7	35	theme	higher	1430:1435	arg1	effect					1437:1442	higher effect	1430:1442	higher effect	1430:1442	FTIR analysis of biochemical composition of Mucoromycota fungi and biomass yield showed that variation in inorganic phosphorus had higher effect when inorganic nitrogen source-ammonium sulphate-was used.
32569317	7	36	theme	FTIR	1299:1302	arg1	analysis					1304:1311	FTIR analysis	1299:1311	FTIR analysis of biochemical composition of Mucoromycota fungi and biomass yield	1299:1378	FTIR analysis of biochemical composition of Mucoromycota fungi and biomass yield showed that variation in inorganic phosphorus had higher effect when inorganic nitrogen source-ammonium sulphate-was used.
32569317	5	37	theme	metabolites	1063:1073	arg1	extraction					1049:1058	tedious extraction	1041:1058	tedious extraction of metabolites	1041:1073	This approach allows single-step biochemical fingerprinting of either fungal biomass or growth media without tedious extraction of metabolites.
32569317	6	38	theme	phosphorus	1157:1166	arg1	sources					1115:1121	nitrogen sources	1106:1121	nitrogen sources	1106:1121	The influence of two types of nitrogen sources and different levels of inorganic phosphorus on the co-production of lipids, chitin/chitosan and polyphosphates for nine different oleaginous Mucoromycota fungi was evaluated.
32569317	6	38	theme	phosphorus	1157:1166	arg1	levels					1137:1142	different levels	1127:1142	different levels of inorganic phosphorus	1127:1166	The influence of two types of nitrogen sources and different levels of inorganic phosphorus on the co-production of lipids, chitin/chitosan and polyphosphates for nine different oleaginous Mucoromycota fungi was evaluated.
32569317	8	39	theme	Amylomyces	1956:1965	arg1	rouxii					1967:1972	Amylomyces rouxii	1956:1972	Amylomyces rouxii	1956:1972	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	5	40	theme	growth	1020:1025	arg1	media					1027:1031	growth media	1020:1031	growth media	1020:1031	This approach allows single-step biochemical fingerprinting of either fungal biomass or growth media without tedious extraction of metabolites.
32569317	8	41	theme	high-value	2163:2172	arg1	producers					2180:2188	high-value lipid producers	2163:2188	high-value lipid producers	2163:2188	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	2	42	theme	Mucoromycota	491:502	arg1	fungi					504:508	Mucoromycota fungi	491:508	Mucoromycota fungi	491:508	However, as far as commercialisation is concerned, establishing industrial biotechnological processes based on Mucoromycota fungi is still challenging due to the high production costs compared to the final product value.
32569317	8	43	theme	phosphorus	1663:1672	arg1	limitation					1674:1683	phosphorus limitation	1663:1683	phosphorus limitation	1663:1683	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	0	44	theme	FTIR	21:24	arg1	screening					45:53	FTIR spectroscopy-based screening	21:53	FTIR spectroscopy-based screening	21:53	Microcultivation and FTIR spectroscopy-based screening revealed a nutrient-induced co-production of high-value metabolites in oleaginous Mucoromycota fungi.
32569317	6	45	theme	different	1127:1135	arg1	levels					1137:1142	different levels	1127:1142	different levels of inorganic phosphorus	1127:1166	The influence of two types of nitrogen sources and different levels of inorganic phosphorus on the co-production of lipids, chitin/chitosan and polyphosphates for nine different oleaginous Mucoromycota fungi was evaluated.
32569317	2	46	theme	biotechnological	455:470	arg1	processes					472:480	industrial biotechnological processes	444:480	industrial biotechnological processes based on Mucoromycota fungi	444:508	However, as far as commercialisation is concerned, establishing industrial biotechnological processes based on Mucoromycota fungi is still challenging due to the high production costs compared to the final product value.
32569317	6	47	theme	sources	1115:1121	arg1	types					1097:1101	two types	1093:1101	two types of nitrogen sources and different levels of inorganic phosphorus	1093:1166	The influence of two types of nitrogen sources and different levels of inorganic phosphorus on the co-production of lipids, chitin/chitosan and polyphosphates for nine different oleaginous Mucoromycota fungi was evaluated.
32569317	3	48	theme	valuable	691:698	arg1	product					700:706	more than one valuable product	677:706	more than one valuable product	677:706	Therefore, the development of co-production concept is highly desired since more than one valuable product could be produced at the time and the process has a potentially higher viability.
32569317	8	49	theme	negative	2430:2437	arg1	impact					2439:2444	negative impact	2430:2444	negative impact on the growth and lipid accumulation	2430:2481	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	8	50	theme	Mortierella	2213:2223	arg1	hyalina					2225:2231	Mortierella hyalina	2213:2231	Mortierella hyalina	2213:2231	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	8	51	theme	Rhizopus	1751:1758	arg1	stolonifer					1760:1769	Rhizopus stolonifer	1751:1769	Rhizopus stolonifer	1751:1769	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	1	52	theme	important	273:281	arg1	alcohols					352:359	alcohols	352:359	alcohols	352:359	Mucoromycota fungi possess a versatile metabolism and can utilize various substrates for production of industrially important products, such as lipids, chitin/chitosan, polyphosphates, pigments, alcohols and organic acids.
32569317	1	52	theme	important	273:281	arg1	products					283:290	industrially important products	260:290	industrially important products	260:290	Mucoromycota fungi possess a versatile metabolism and can utilize various substrates for production of industrially important products, such as lipids, chitin/chitosan, polyphosphates, pigments, alcohols and organic acids.
32569317	1	52	theme	important	273:281	arg1	polyphosphates					326:339	polyphosphates	326:339	polyphosphates	326:339	Mucoromycota fungi possess a versatile metabolism and can utilize various substrates for production of industrially important products, such as lipids, chitin/chitosan, polyphosphates, pigments, alcohols and organic acids.
32569317	1	52	theme	important	273:281	arg1	acids					373:377	organic acids	365:377	organic acids	365:377	Mucoromycota fungi possess a versatile metabolism and can utilize various substrates for production of industrially important products, such as lipids, chitin/chitosan, polyphosphates, pigments, alcohols and organic acids.
32569317	1	52	theme	important	273:281	arg1	pigments					342:349	pigments	342:349	pigments	342:349	Mucoromycota fungi possess a versatile metabolism and can utilize various substrates for production of industrially important products, such as lipids, chitin/chitosan, polyphosphates, pigments, alcohols and organic acids.
32569317	1	52	theme	important	273:281	arg1	lipids					301:306	lipids	301:306	lipids	301:306	Mucoromycota fungi possess a versatile metabolism and can utilize various substrates for production of industrially important products, such as lipids, chitin/chitosan, polyphosphates, pigments, alcohols and organic acids.
32569317	1	52	theme	important	273:281	arg1	chitin/chitosan					309:323	chitin/chitosan	309:323	chitin/chitosan	309:323	Mucoromycota fungi possess a versatile metabolism and can utilize various substrates for production of industrially important products, such as lipids, chitin/chitosan, polyphosphates, pigments, alcohols and organic acids.
32569317	8	53	theme	phosphorus	2300:2309	arg1	concentrations					2272:2285	high concentrations	2267:2285	high concentrations of inorganic phosphorus	2267:2309	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	1	54	theme	versatile	186:194	arg1	metabolism					196:205	a versatile metabolism	184:205	a versatile metabolism	184:205	Mucoromycota fungi possess a versatile metabolism and can utilize various substrates for production of industrially important products, such as lipids, chitin/chitosan, polyphosphates, pigments, alcohols and organic acids.
32569317	9	55	from	chitin/chitosan	2596:2610	arg1	process					2655:2661	a single cultivation process	2634:2661	a single cultivation process	2634:2661	FTIR spectroscopy revealed the co-production potential of several oleaginous Mucoromycota fungi forming lipids, chitin/chitosan and polyphosphates in a single cultivation process.
32569317	2	56	theme	industrial	444:453	arg1	processes					472:480	industrial biotechnological processes	444:480	industrial biotechnological processes based on Mucoromycota fungi	444:508	However, as far as commercialisation is concerned, establishing industrial biotechnological processes based on Mucoromycota fungi is still challenging due to the high production costs compared to the final product value.
32569317	4	57	theme	high	846:849	arg1	approach					862:869	a high throughput approach	844:869	a high throughput approach consisting of micro-titre cultivation and FTIR spectroscopy	844:929	To develop such biotechnological strategy, we applied a high throughput approach consisting of micro-titre cultivation and FTIR spectroscopy.
32569317	0	58	theme	nutrient-induced	66:81	arg1	co-production					83:95	a nutrient-induced co-production	64:95	a nutrient-induced co-production of high-value metabolites in oleaginous Mucoromycota fungi	64:154	Microcultivation and FTIR spectroscopy-based screening revealed a nutrient-induced co-production of high-value metabolites in oleaginous Mucoromycota fungi.
32569317	7	59	theme	yield	1374:1378	arg1	composition					1328:1338	biochemical composition	1316:1338	biochemical composition of Mucoromycota fungi and biomass yield	1316:1378	FTIR analysis of biochemical composition of Mucoromycota fungi and biomass yield showed that variation in inorganic phosphorus had higher effect when inorganic nitrogen source-ammonium sulphate-was used.
32569317	7	60	theme	biochemical	1316:1326	arg1	composition					1328:1338	biochemical composition	1316:1338	biochemical composition of Mucoromycota fungi and biomass yield	1316:1378	FTIR analysis of biochemical composition of Mucoromycota fungi and biomass yield showed that variation in inorganic phosphorus had higher effect when inorganic nitrogen source-ammonium sulphate-was used.
32569317	9	61	theme	FTIR	2484:2487	arg1	spectroscopy					2489:2500	FTIR spectroscopy	2484:2500	FTIR spectroscopy	2484:2500	FTIR spectroscopy revealed the co-production potential of several oleaginous Mucoromycota fungi forming lipids, chitin/chitosan and polyphosphates in a single cultivation process.
32569317	8	62	theme	lipid	2464:2468	arg1	accumulation					2470:2481	lipid accumulation	2464:2481	lipid accumulation	2464:2481	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	8	63	theme	high	2267:2270	arg1	concentrations					2272:2285	high concentrations	2267:2285	high concentrations of inorganic phosphorus	2267:2309	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	8	64	theme	Absidia	1791:1797	arg1	chitin/chitosan					1847:1861	Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan	1791:1861	Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides	1791:1953	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	8	65	from	impact	2439:2444	arg1	growth					2453:2458	the growth	2449:2458	the growth	2449:2458	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	8	65	from	impact	2439:2444	arg1	accumulation					2470:2481	lipid accumulation	2464:2481	lipid accumulation	2464:2481	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	3	66	theme	higher	772:777	arg1	viability					779:787	a potentially higher viability	758:787	a potentially higher viability	758:787	Therefore, the development of co-production concept is highly desired since more than one valuable product could be produced at the time and the process has a potentially higher viability.
32569317	9	67	theme	co-production	2515:2527	arg1	potential					2529:2537	the co-production potential	2511:2537	the co-production potential of several oleaginous Mucoromycota fungi forming lipids, chitin/chitosan and polyphosphates in a single cultivation process	2511:2661	FTIR spectroscopy revealed the co-production potential of several oleaginous Mucoromycota fungi forming lipids, chitin/chitosan and polyphosphates in a single cultivation process.
32569317	4	68	theme	micro-titre	885:895	arg1	cultivation					897:907	micro-titre cultivation	885:907	micro-titre cultivation	885:907	To develop such biotechnological strategy, we applied a high throughput approach consisting of micro-titre cultivation and FTIR spectroscopy.
32569317	6	69	theme	Mucoromycota	1265:1276	arg1	fungi					1278:1282	nine different oleaginous Mucoromycota fungi	1239:1282	nine different oleaginous Mucoromycota fungi	1239:1282	The influence of two types of nitrogen sources and different levels of inorganic phosphorus on the co-production of lipids, chitin/chitosan and polyphosphates for nine different oleaginous Mucoromycota fungi was evaluated.
32569317	7	70	theme	composition	1328:1338	arg1	analysis					1304:1311	FTIR analysis	1299:1311	FTIR analysis of biochemical composition of Mucoromycota fungi and biomass yield	1299:1378	FTIR analysis of biochemical composition of Mucoromycota fungi and biomass yield showed that variation in inorganic phosphorus had higher effect when inorganic nitrogen source-ammonium sulphate-was used.
32569317	1	71	theme	various	223:229	arg1	substrates					231:240	various substrates	223:240	various substrates for production of industrially important products, such as lipids, chitin/chitosan, polyphosphates, pigments, alcohols and organic acids	223:377	Mucoromycota fungi possess a versatile metabolism and can utilize various substrates for production of industrially important products, such as lipids, chitin/chitosan, polyphosphates, pigments, alcohols and organic acids.
32569317	7	72	from	variation	1392:1400	arg1	phosphorus					1415:1424	inorganic phosphorus	1405:1424	inorganic phosphorus	1405:1424	FTIR analysis of biochemical composition of Mucoromycota fungi and biomass yield showed that variation in inorganic phosphorus had higher effect when inorganic nitrogen source-ammonium sulphate-was used.
32569317	6	73	theme	different	1244:1252	arg1	fungi					1278:1282	nine different oleaginous Mucoromycota fungi	1239:1282	nine different oleaginous Mucoromycota fungi	1239:1282	The influence of two types of nitrogen sources and different levels of inorganic phosphorus on the co-production of lipids, chitin/chitosan and polyphosphates for nine different oleaginous Mucoromycota fungi was evaluated.
32569317	9	74	theme	oleaginous	2550:2559	arg1	fungi					2574:2578	several oleaginous Mucoromycota fungi	2542:2578	several oleaginous Mucoromycota fungi forming lipids, chitin/chitosan and polyphosphates in a single cultivation process	2542:2661	FTIR spectroscopy revealed the co-production potential of several oleaginous Mucoromycota fungi forming lipids, chitin/chitosan and polyphosphates in a single cultivation process.
32569317	8	75	theme	chitin/chitosan	1847:1861	arg1	circinelloides					1940:1953	Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides	1791:1953	Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides	1791:1953	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	8	76	theme	biomass	1570:1576	arg1	yield					1578:1582	almost double biomass yield	1556:1582	almost double biomass yield	1556:1582	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	8	77	theme	ammonium	2330:2337	arg1	sulphate					2339:2346	ammonium sulphate	2330:2346	ammonium sulphate	2330:2346	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	9	78	theme	fungi	2574:2578	arg1	potential					2529:2537	the co-production potential	2511:2537	the co-production potential of several oleaginous Mucoromycota fungi forming lipids, chitin/chitosan and polyphosphates in a single cultivation process	2511:2661	FTIR spectroscopy revealed the co-production potential of several oleaginous Mucoromycota fungi forming lipids, chitin/chitosan and polyphosphates in a single cultivation process.
32569317	2	79	theme	product	586:592	arg1	value					594:598	the final product value	576:598	the final product value	576:598	However, as far as commercialisation is concerned, establishing industrial biotechnological processes based on Mucoromycota fungi is still challenging due to the high production costs compared to the final product value.
32569317	8	80	theme	phosphorus	2107:2116	arg1	concentration					2080:2092	high concentration	2075:2092	high concentration of inorganic phosphorus	2075:2116	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	3	81	theme	co-production	631:643	arg1	concept					645:651	co-production concept	631:651	co-production concept	631:651	Therefore, the development of co-production concept is highly desired since more than one valuable product could be produced at the time and the process has a potentially higher viability.
32569317	8	82	theme	other	1596:1600	arg1	strains					1602:1608	other strains	1596:1608	other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield	1596:1722	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	3	83	contain	has	754:756	arg2	viability					779:787	a potentially higher viability	758:787	a potentially higher viability	758:787	Therefore, the development of co-production concept is highly desired since more than one valuable product could be produced at the time and the process has a potentially higher viability.
32569317	3	83	contain	has	754:756	arg1	process					746:752	the process	742:752	the process	742:752	Therefore, the development of co-production concept is highly desired since more than one valuable product could be produced at the time and the process has a potentially higher viability.
32569317	5	84	theme	fungal	1002:1007	arg1	biomass					1009:1015	fungal biomass	1002:1015	fungal biomass	1002:1015	This approach allows single-step biochemical fingerprinting of either fungal biomass or growth media without tedious extraction of metabolites.
32569317	6	85	theme	nitrogen	1106:1113	arg1	sources					1115:1121	nitrogen sources	1106:1121	nitrogen sources	1106:1121	The influence of two types of nitrogen sources and different levels of inorganic phosphorus on the co-production of lipids, chitin/chitosan and polyphosphates for nine different oleaginous Mucoromycota fungi was evaluated.
32569317	5	86	theme	tedious	1041:1047	arg1	extraction					1049:1058	tedious extraction	1041:1058	tedious extraction of metabolites	1041:1073	This approach allows single-step biochemical fingerprinting of either fungal biomass or growth media without tedious extraction of metabolites.
32569317	8	87	contain	had	1685:1687	arg1	limitation					1674:1683	phosphorus limitation	1663:1683	phosphorus limitation	1663:1683	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	8	87	contain	had	1685:1687	arg2	effect					1696:1701	little effect	1689:1701	little effect	1689:1701	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	8	88	used	used	1633:1636	arg2	source					1650:1655	nitrogen source	1641:1655	nitrogen source	1641:1655	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	8	88	used	used	1633:1636	arg2	extract					1621:1627	yeast extract	1615:1627	yeast extract	1615:1627	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	8	89	theme	high	2075:2078	arg1	concentration					2080:2092	high concentration	2075:2092	high concentration of inorganic phosphorus	2075:2116	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	6	90	theme	types	1097:1101	arg1	influence					1080:1088	The influence	1076:1088	The influence of two types of nitrogen sources and different levels of inorganic phosphorus on the co-production of lipids, chitin/chitosan and polyphosphates for nine different oleaginous Mucoromycota fungi	1076:1282	The influence of two types of nitrogen sources and different levels of inorganic phosphorus on the co-production of lipids, chitin/chitosan and polyphosphates for nine different oleaginous Mucoromycota fungi was evaluated.
32569317	2	91	theme	production	547:556	arg1	costs					558:562	the high production costs	538:562	the high production costs compared to the final product value	538:598	However, as far as commercialisation is concerned, establishing industrial biotechnological processes based on Mucoromycota fungi is still challenging due to the high production costs compared to the final product value.
32569317	8	92	theme	inorganic	2374:2382	arg1	phosphorus					2384:2393	inorganic phosphorus	2374:2393	inorganic phosphorus combined with yeast extract	2374:2421	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	0	93	theme	metabolites	111:121	arg1	co-production					83:95	a nutrient-induced co-production	64:95	a nutrient-induced co-production of high-value metabolites in oleaginous Mucoromycota fungi	64:154	Microcultivation and FTIR spectroscopy-based screening revealed a nutrient-induced co-production of high-value metabolites in oleaginous Mucoromycota fungi.
32569317	8	94	dep	circinelloides	1735:1748	arg1	2					1726:1726	2	1726:1726	2	1726:1726	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	2	95	dep	challenging	519:529	arg1	concerned					420:428	concerned	420:428	is concerned	417:428	However, as far as commercialisation is concerned, establishing industrial biotechnological processes based on Mucoromycota fungi is still challenging due to the high production costs compared to the final product value.
32569317	8	96	theme	inorganic	1908:1916	arg1	phosphorus					1918:1927	inorganic phosphorus	1908:1927	inorganic phosphorus	1908:1927	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	5	97	theme	biochemical	965:975	arg1	fingerprinting					977:990	single-step biochemical fingerprinting	953:990	single-step biochemical fingerprinting of either fungal biomass or growth media	953:1031	This approach allows single-step biochemical fingerprinting of either fungal biomass or growth media without tedious extraction of metabolites.
32569317	7	98	used	used	1497:1500	arg2	sulphate-was					1484:1495	inorganic nitrogen source-ammonium sulphate-was	1449:1495	inorganic nitrogen source-ammonium sulphate-was	1449:1495	FTIR analysis of biochemical composition of Mucoromycota fungi and biomass yield showed that variation in inorganic phosphorus had higher effect when inorganic nitrogen source-ammonium sulphate-was used.
32569317	0	99	theme	oleaginous	126:135	arg1	fungi					150:154	oleaginous Mucoromycota fungi	126:154	oleaginous Mucoromycota fungi	126:154	Microcultivation and FTIR spectroscopy-based screening revealed a nutrient-induced co-production of high-value metabolites in oleaginous Mucoromycota fungi.
32569317	9	100	theme	cultivation	2643:2653	arg1	process					2655:2661	a single cultivation process	2634:2661	a single cultivation process	2634:2661	FTIR spectroscopy revealed the co-production potential of several oleaginous Mucoromycota fungi forming lipids, chitin/chitosan and polyphosphates in a single cultivation process.
32569317	7	101	dep	showed	1380:1385	arg1	had					1426:1428	had	1426:1428	showed that variation in inorganic phosphorus had higher effect when inorganic nitrogen source-ammonium sulphate-was used	1380:1500	FTIR analysis of biochemical composition of Mucoromycota fungi and biomass yield showed that variation in inorganic phosphorus had higher effect when inorganic nitrogen source-ammonium sulphate-was used.
32569317	8	102	theme	nitrogen	1641:1648	arg1	source					1650:1655	nitrogen source	1641:1655	nitrogen source	1641:1655	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	8	102	theme	nitrogen	1641:1648	arg1	extract					1621:1627	yeast extract	1615:1627	yeast extract	1615:1627	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	4	103	theme	such	801:804	arg1	strategy					823:830	such biotechnological strategy	801:830	such biotechnological strategy	801:830	To develop such biotechnological strategy, we applied a high throughput approach consisting of micro-titre cultivation and FTIR spectroscopy.
32569317	7	104	theme	inorganic	1449:1457	arg1	sulphate-was					1484:1495	inorganic nitrogen source-ammonium sulphate-was	1449:1495	inorganic nitrogen source-ammonium sulphate-was	1449:1495	FTIR analysis of biochemical composition of Mucoromycota fungi and biomass yield showed that variation in inorganic phosphorus had higher effect when inorganic nitrogen source-ammonium sulphate-was used.
32569317	8	105	used	used	2122:2125	arg2	concentration					2080:2092	high concentration	2075:2092	high concentration of inorganic phosphorus	2075:2116	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	8	106	theme	Mucor	1934:1938	arg1	circinelloides					1940:1953	Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides	1791:1953	Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides	1791:1953	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	8	107	theme	lipid	2174:2178	arg1	producers					2180:2188	high-value lipid producers	2163:2188	high-value lipid producers	2163:2188	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	6	108	from	influence	1080:1088	arg1	co-production					1175:1187	the co-production	1171:1187	the co-production of lipids, chitin/chitosan and polyphosphates for nine different oleaginous Mucoromycota fungi	1171:1282	The influence of two types of nitrogen sources and different levels of inorganic phosphorus on the co-production of lipids, chitin/chitosan and polyphosphates for nine different oleaginous Mucoromycota fungi was evaluated.
32569317	8	109	dep	Lichtheimia	1810:1820	arg1	corymbifera					1822:1832	corymbifera	1822:1832	corymbifera	1822:1832	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	0	110	theme	spectroscopy-based	26:43	arg1	screening					45:53	FTIR spectroscopy-based screening	21:53	FTIR spectroscopy-based screening	21:53	Microcultivation and FTIR spectroscopy-based screening revealed a nutrient-induced co-production of high-value metabolites in oleaginous Mucoromycota fungi.
32569317	5	111	theme	media	1027:1031	arg1	fingerprinting					977:990	single-step biochemical fingerprinting	953:990	single-step biochemical fingerprinting of either fungal biomass or growth media	953:1031	This approach allows single-step biochemical fingerprinting of either fungal biomass or growth media without tedious extraction of metabolites.
32569317	6	112	theme	levels	1137:1142	arg1	types					1097:1101	two types	1093:1101	two types of nitrogen sources and different levels of inorganic phosphorus	1093:1166	The influence of two types of nitrogen sources and different levels of inorganic phosphorus on the co-production of lipids, chitin/chitosan and polyphosphates for nine different oleaginous Mucoromycota fungi was evaluated.
32569317	8	113	theme	little	1689:1694	arg1	effect					1696:1701	little effect	1689:1701	little effect	1689:1701	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	7	114	theme	biomass	1366:1372	arg1	yield					1374:1378	biomass yield	1366:1378	biomass yield	1366:1378	FTIR analysis of biochemical composition of Mucoromycota fungi and biomass yield showed that variation in inorganic phosphorus had higher effect when inorganic nitrogen source-ammonium sulphate-was used.
32569317	8	115	theme	biomass	2136:2142	arg1	Mortierella					2190:2200	(4) the biomass and lipid yield of high-value lipid producers Mortierella	2128:2200	(4) the biomass and lipid yield of high-value lipid producers Mortierella alpina	2128:2207	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	7	116	theme	inorganic	1405:1413	arg1	phosphorus					1415:1424	inorganic phosphorus	1405:1424	inorganic phosphorus	1405:1424	FTIR analysis of biochemical composition of Mucoromycota fungi and biomass yield showed that variation in inorganic phosphorus had higher effect when inorganic nitrogen source-ammonium sulphate-was used.
32569317	4	117	theme	throughput	851:860	arg1	approach					862:869	a high throughput approach	844:869	a high throughput approach consisting of micro-titre cultivation and FTIR spectroscopy	844:929	To develop such biotechnological strategy, we applied a high throughput approach consisting of micro-titre cultivation and FTIR spectroscopy.
32569317	8	118	theme	producers	2180:2188	arg1	biomass					2136:2142	the biomass	2132:2142	(4) the biomass	2128:2142	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	8	118	theme	producers	2180:2188	arg1	yield					2154:2158	lipid yield	2148:2158	lipid yield	2148:2158	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	0	119	theme	Mucoromycota	137:148	arg1	fungi					150:154	oleaginous Mucoromycota fungi	126:154	oleaginous Mucoromycota fungi	126:154	Microcultivation and FTIR spectroscopy-based screening revealed a nutrient-induced co-production of high-value metabolites in oleaginous Mucoromycota fungi.
32569317	8	120	theme	lipid	2148:2152	arg1	yield					2154:2158	lipid yield	2148:2158	lipid yield	2148:2158	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	8	121	theme	yeast	2409:2413	arg1	extract					2415:2421	yeast extract	2409:2421	yeast extract	2409:2421	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	6	122	theme	polyphosphates	1220:1233	arg1	co-production					1175:1187	the co-production	1171:1187	the co-production of lipids, chitin/chitosan and polyphosphates for nine different oleaginous Mucoromycota fungi	1171:1282	The influence of two types of nitrogen sources and different levels of inorganic phosphorus on the co-production of lipids, chitin/chitosan and polyphosphates for nine different oleaginous Mucoromycota fungi was evaluated.
32569317	8	123	theme	phosphorus	2384:2393	arg1	amount					2364:2369	the same amount	2355:2369	the same amount of inorganic phosphorus combined with yeast extract	2355:2421	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	8	123	theme	phosphorus	2384:2393	arg1	phosphorus					2384:2393	inorganic phosphorus	2374:2393	inorganic phosphorus combined with yeast extract	2374:2421	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	8	124	theme	biomass	1710:1716	arg1	yield					1718:1722	the biomass yield	1706:1722	the biomass yield	1706:1722	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	1	125	theme	organic	365:371	arg1	acids					373:377	organic acids	365:377	organic acids	365:377	Mucoromycota fungi possess a versatile metabolism and can utilize various substrates for production of industrially important products, such as lipids, chitin/chitosan, polyphosphates, pigments, alcohols and organic acids.
32569317	6	126	theme	chitin/chitosan	1200:1214	arg1	co-production					1175:1187	the co-production	1171:1187	the co-production of lipids, chitin/chitosan and polyphosphates for nine different oleaginous Mucoromycota fungi	1171:1282	The influence of two types of nitrogen sources and different levels of inorganic phosphorus on the co-production of lipids, chitin/chitosan and polyphosphates for nine different oleaginous Mucoromycota fungi was evaluated.
32569317	8	127	theme	Umbelopsis	1529:1538	arg1	vinacea					1540:1546	(1) Umbelopsis vinacea	1525:1546	(1) Umbelopsis vinacea	1525:1546	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	8	128	theme	Amylomyces	1772:1781	arg1	rouxii					1783:1788	Amylomyces rouxii	1772:1788	Amylomyces rouxii	1772:1788	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	1	129	theme	products	283:290	arg1	production					246:255	production	246:255	production of industrially important products, such as lipids, chitin/chitosan, polyphosphates, pigments, alcohols and organic acids	246:377	Mucoromycota fungi possess a versatile metabolism and can utilize various substrates for production of industrially important products, such as lipids, chitin/chitosan, polyphosphates, pigments, alcohols and organic acids.
32569317	6	130	theme	lipids	1192:1197	arg1	co-production					1175:1187	the co-production	1171:1187	the co-production of lipids, chitin/chitosan and polyphosphates for nine different oleaginous Mucoromycota fungi	1171:1282	The influence of two types of nitrogen sources and different levels of inorganic phosphorus on the co-production of lipids, chitin/chitosan and polyphosphates for nine different oleaginous Mucoromycota fungi was evaluated.
32569317	8	131	theme	yield	2154:2158	arg1	Mortierella					2190:2200	(4) the biomass and lipid yield of high-value lipid producers Mortierella	2128:2200	(4) the biomass and lipid yield of high-value lipid producers Mortierella alpina	2128:2207	It was observed that: (1) Umbelopsis vinacea reached almost double biomass yield compared to other strains when yeast extract was used as nitrogen source while phosphorus limitation had little effect on the biomass yield; (2) Mucor circinelloides, Rhizopus stolonifer, Amylomyces rouxii, Absidia glauca and Lichtheimia corymbifera overproduced chitin/chitosan under the low pH caused by the limitation of inorganic phosphorus; (3) Mucor circinelloides, Amylomyces rouxii, Rhizopus stolonifer and Absidia glauca were able to store polyphosphates in addition to lipids when high concentration of inorganic phosphorus was used; (4) the biomass and lipid yield of high-value lipid producers Mortierella alpina and Mortierella hyalina were significantly increased when high concentrations of inorganic phosphorus were combined with ammonium sulphate, while the same amount of inorganic phosphorus combined with yeast extract showed negative impact on the growth and lipid accumulation.
32569317	1	132	contain	possess	176:182	arg1	fungi					170:174	Mucoromycota fungi	157:174	Mucoromycota fungi	157:174	Mucoromycota fungi possess a versatile metabolism and can utilize various substrates for production of industrially important products, such as lipids, chitin/chitosan, polyphosphates, pigments, alcohols and organic acids.
32569317	1	132	contain	possess	176:182	arg2	metabolism					196:205	a versatile metabolism	184:205	a versatile metabolism	184:205	Mucoromycota fungi possess a versatile metabolism and can utilize various substrates for production of industrially important products, such as lipids, chitin/chitosan, polyphosphates, pigments, alcohols and organic acids.
31730027	3	0	theme	Jiangxi	296:302	arg1	Province					304:311	Jiangxi Province	296:311	Jiangxi Province	296:311	A novel filamentous, spore-forming, Gram-stain-positive bacterium, designated SCAWS-G2T, was isolated from red soil in Jiangxi Province, PR China.
31730027	3	0	theme	Jiangxi	296:302	arg1	China					317:321	PR China	314:321	PR China	314:321	A novel filamentous, spore-forming, Gram-stain-positive bacterium, designated SCAWS-G2T, was isolated from red soil in Jiangxi Province, PR China.
31730027	13	1	theme	gen.	1656:1659	arg1	nov.					1661:1664	Ktedonosporobacter rubrisoli gen. nov.	1627:1664	Ktedonosporobacter rubrisoli gen. nov.	1627:1664	Based on all these data, we propose that strain SCAWS-G2T represents a novel genus and species, Ktedonosporobacter rubrisoli gen. nov., sp.
31730027	13	1	theme	gen.	1656:1659	arg1	genus					1608:1612	a novel genus	1600:1612	a novel genus	1600:1612	Based on all these data, we propose that strain SCAWS-G2T represents a novel genus and species, Ktedonosporobacter rubrisoli gen. nov., sp.
31730027	8	2	theme	genome-wide	930:940	arg1	values					970:975	The genome-wide average nucleotide identity values	926:975	The genome-wide average nucleotide identity values between strain SCAWS-G2T and the related type strains	926:1029	The genome-wide average nucleotide identity values between strain SCAWS-G2T and the related type strains were <68.2 %.
31730027	8	2	theme	genome-wide	930:940	arg1	%					1042:1042	<68.2 %	1036:1042	<68.2 %	1036:1042	The genome-wide average nucleotide identity values between strain SCAWS-G2T and the related type strains were <68.2 %.
31730027	6	3	theme	strain	648:653	arg1	SCAWS-G2T					655:663	strain SCAWS-G2T	648:663	strain SCAWS-G2T	648:663	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SCAWS-G2T formed a distinct lineage within the order Ktedonobacterales, showing <89.2 % sequence similarities to the recognized taxa of this order.
31730027	7	4	theme	phylogenomic	826:837	arg1	tree					839:842	The whole-genome based phylogenomic tree	803:842	The whole-genome based phylogenomic tree	803:842	The whole-genome based phylogenomic tree separated strain SCAWS-G2T from the recognized families within Ktedonobacterales.
31730027	12	5	theme	major	1469:1473	arg1	C16 					1492:1495	C16 	1492:1495	C16 	1492:1495	The major fatty acids were C16 : 1 2-OH, C16 : 0 and iso-C17 : 0.
31730027	12	5	theme	major	1469:1473	arg1	acids					1481:1485	The major fatty acids	1465:1485	The major fatty acids	1465:1485	The major fatty acids were C16 : 1 2-OH, C16 : 0 and iso-C17 : 0.
31730027	13	6	theme	Ktedonosporobacter	1627:1644	arg1	nov.					1661:1664	Ktedonosporobacter rubrisoli gen. nov.	1627:1664	Ktedonosporobacter rubrisoli gen. nov.	1627:1664	Based on all these data, we propose that strain SCAWS-G2T represents a novel genus and species, Ktedonosporobacter rubrisoli gen. nov., sp.
31730027	13	6	theme	Ktedonosporobacter	1627:1644	arg1	genus					1608:1612	a novel genus	1600:1612	a novel genus	1600:1612	Based on all these data, we propose that strain SCAWS-G2T represents a novel genus and species, Ktedonosporobacter rubrisoli gen. nov., sp.
31730027	8	7	theme	average	942:948	arg1	values					970:975	The genome-wide average nucleotide identity values	926:975	The genome-wide average nucleotide identity values between strain SCAWS-G2T and the related type strains	926:1029	The genome-wide average nucleotide identity values between strain SCAWS-G2T and the related type strains were <68.2 %.
31730027	8	7	theme	average	942:948	arg1	%					1042:1042	<68.2 %	1036:1042	<68.2 %	1036:1042	The genome-wide average nucleotide identity values between strain SCAWS-G2T and the related type strains were <68.2 %.
31730027	4	8	dep	50 mM	402:406	arg1	to					399:400	to	399:400	to	399:400	The strain grew at 25-45 °C and at pH 4.0-7.0, and was able to tolerate up to 50 mM Zn2+.
31730027	11	9	theme	cell-wall	1412:1420	arg1	galactose					1441:1449	galactose	1441:1449	galactose	1441:1449	The peptidoglycan amino acids contained ornithine, glycine, glutamic acid and alanine, and the cell-wall sugars were mainly galactose and rhamnose.
31730027	11	9	theme	cell-wall	1412:1420	arg1	sugars					1422:1427	the cell-wall sugars	1408:1427	the cell-wall sugars	1408:1427	The peptidoglycan amino acids contained ornithine, glycine, glutamic acid and alanine, and the cell-wall sugars were mainly galactose and rhamnose.
31730027	5	10	theme	16S	515:517	arg1	genes					524:528	four 16S rRNA genes	510:528	four 16S rRNA genes with three sequence types (0.4-0.8 % differences)	510:578	The complete genome of strain SCAWS-G2T was a circular chromosome of ~11.34 Mb, which contained four 16S rRNA genes with three sequence types (0.4-0.8 % differences).
31730027	3	11	theme	filamentous	185:195	arg1	bacterium					233:241	A novel filamentous, spore-forming, Gram-stain-positive bacterium	177:241	A novel filamentous, spore-forming, Gram-stain-positive bacterium	177:241	A novel filamentous, spore-forming, Gram-stain-positive bacterium, designated SCAWS-G2T, was isolated from red soil in Jiangxi Province, PR China.
31730027	6	12	theme	sequence	743:750	arg1	similarities					752:763	<89.2 % sequence similarities	735:763	<89.2 % sequence similarities to the recognized taxa of this order	735:800	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SCAWS-G2T formed a distinct lineage within the order Ktedonobacterales, showing <89.2 % sequence similarities to the recognized taxa of this order.
31730027	9	13	theme	phenotypic	1127:1136	arg1	characteristics					1138:1152	phenotypic characteristics	1127:1152	phenotypic characteristics	1127:1152	The strain can also be differentiated from the recognized families by a number of phenotypic characteristics.
31730027	14	14	dep	nov.	1671:1674	arg1	fam					1723:1725	the new family Ktedonosporobacteraceae fam	1684:1725	the new family Ktedonosporobacteraceae fam	1684:1725	nov., within the new family Ktedonosporobacteraceae fam.
31730027	8	15	theme	related	1010:1016	arg1	strains					1023:1029	the related type strains	1006:1029	the related type strains	1006:1029	The genome-wide average nucleotide identity values between strain SCAWS-G2T and the related type strains were <68.2 %.
31730027	5	16	theme	sequence	541:548	arg1	 differences					566:577	0.4-0.8 % differences	557:577	0.4-0.8 % differences	557:577	The complete genome of strain SCAWS-G2T was a circular chromosome of ~11.34 Mb, which contained four 16S rRNA genes with three sequence types (0.4-0.8 % differences).
31730027	5	16	theme	sequence	541:548	arg1	types					550:554	three sequence types	535:554	three sequence types (0.4-0.8 % differences)	535:578	The complete genome of strain SCAWS-G2T was a circular chromosome of ~11.34 Mb, which contained four 16S rRNA genes with three sequence types (0.4-0.8 % differences).
31730027	14	17	theme	Ktedonosporobacteraceae	1699:1721	arg1	fam					1723:1725	the new family Ktedonosporobacteraceae fam	1684:1725	the new family Ktedonosporobacteraceae fam	1684:1725	nov., within the new family Ktedonosporobacteraceae fam.
31730027	10	18	theme	unidentified	1297:1308	arg1	lipid					1310:1314	one unidentified lipid	1293:1314	one unidentified lipid	1293:1314	The polar lipids of SCAWS-G2T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, seven unidentified glycolipids and one unidentified lipid.
31730027	15	19	theme	order	1740:1744	arg1	nov.					1728:1731	nov.	1728:1731	nov. of the order	1728:1744	nov. of the order Ktedonobacterales.
31730027	16	20	theme	type	1769:1772	arg1	strain					1774:1779	The type strain	1765:1779	The type strain of Ktedonosporobacter rubrisoli	1765:1811	The type strain of Ktedonosporobacter rubrisoli is SCAWS-G2T (=CGMCC 1.16132T=DSM 105258T).
31730027	16	20	theme	type	1769:1772	arg1	SCAWS-G2T					1816:1824	SCAWS-G2T	1816:1824	SCAWS-G2T (=CGMCC 1.16132T=DSM 105258T)	1816:1854	The type strain of Ktedonosporobacter rubrisoli is SCAWS-G2T (=CGMCC 1.16132T=DSM 105258T).
31730027	6	21	theme	<89.2 	735:740	arg1	%					741:741	%	741:741	%	741:741	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SCAWS-G2T formed a distinct lineage within the order Ktedonobacterales, showing <89.2 % sequence similarities to the recognized taxa of this order.
31730027	1	22	theme	novel	52:56	arg1	representative					58:71	a novel representative	50:71	a novel representative of the class Ktedonobacteria, isolated from red soil,	50:125	nov., a novel representative of the class Ktedonobacteria, isolated from red soil, and proposal of Ktedonosporobacteraceae fam.
31730027	1	22	theme	novel	52:56	arg1	nov.					44:47	nov.	44:47	nov.	44:47	nov., a novel representative of the class Ktedonobacteria, isolated from red soil, and proposal of Ktedonosporobacteraceae fam.
31730027	5	23	theme	strain	437:442	arg1	SCAWS-G2T					444:452	strain SCAWS-G2T	437:452	strain SCAWS-G2T	437:452	The complete genome of strain SCAWS-G2T was a circular chromosome of ~11.34 Mb, which contained four 16S rRNA genes with three sequence types (0.4-0.8 % differences).
31730027	9	24	theme	characteristics	1138:1152	arg1	number					1117:1122	a number	1115:1122	a number of phenotypic characteristics	1115:1152	The strain can also be differentiated from the recognized families by a number of phenotypic characteristics.
31730027	5	25	theme	0.4-0.8 	557:564	arg1	 differences					566:577	0.4-0.8 % differences	557:577	0.4-0.8 % differences	557:577	The complete genome of strain SCAWS-G2T was a circular chromosome of ~11.34 Mb, which contained four 16S rRNA genes with three sequence types (0.4-0.8 % differences).
31730027	5	25	theme	0.4-0.8 	557:564	arg1	types					550:554	three sequence types	535:554	three sequence types (0.4-0.8 % differences)	535:578	The complete genome of strain SCAWS-G2T was a circular chromosome of ~11.34 Mb, which contained four 16S rRNA genes with three sequence types (0.4-0.8 % differences).
31730027	7	26	theme	recognized	880:889	arg1	families					891:898	the recognized families	876:898	the recognized families within Ktedonobacterales	876:923	The whole-genome based phylogenomic tree separated strain SCAWS-G2T from the recognized families within Ktedonobacterales.
31730027	5	27	theme	SCAWS-G2T	444:452	arg1	chromosome					469:478	a circular chromosome	458:478	a circular chromosome	458:478	The complete genome of strain SCAWS-G2T was a circular chromosome of ~11.34 Mb, which contained four 16S rRNA genes with three sequence types (0.4-0.8 % differences).
31730027	5	27	theme	SCAWS-G2T	444:452	arg1	genome					427:432	The complete genome	414:432	The complete genome of strain SCAWS-G2T	414:452	The complete genome of strain SCAWS-G2T was a circular chromosome of ~11.34 Mb, which contained four 16S rRNA genes with three sequence types (0.4-0.8 % differences).
31730027	1	28	theme	Ktedonosporobacteraceae	143:165	arg1	fam					167:169	Ktedonosporobacteraceae fam	143:169	Ktedonosporobacteraceae fam	143:169	nov., a novel representative of the class Ktedonobacteria, isolated from red soil, and proposal of Ktedonosporobacteraceae fam.
31730027	0	29	theme	rubrisoli	19:27	arg1	nov.					34:37	Ktedonosporobacter rubrisoli gen. nov.	0:37	Ktedonosporobacter rubrisoli gen. nov.	0:37	Ktedonosporobacter rubrisoli gen. nov., sp.
31730027	5	30	theme	%	565:565	arg1	 differences					566:577	0.4-0.8 % differences	557:577	0.4-0.8 % differences	557:577	The complete genome of strain SCAWS-G2T was a circular chromosome of ~11.34 Mb, which contained four 16S rRNA genes with three sequence types (0.4-0.8 % differences).
31730027	5	30	theme	%	565:565	arg1	types					550:554	three sequence types	535:554	three sequence types (0.4-0.8 % differences)	535:578	The complete genome of strain SCAWS-G2T was a circular chromosome of ~11.34 Mb, which contained four 16S rRNA genes with three sequence types (0.4-0.8 % differences).
31730027	3	31	theme	novel	179:183	arg1	bacterium					233:241	A novel filamentous, spore-forming, Gram-stain-positive bacterium	177:241	A novel filamentous, spore-forming, Gram-stain-positive bacterium	177:241	A novel filamentous, spore-forming, Gram-stain-positive bacterium, designated SCAWS-G2T, was isolated from red soil in Jiangxi Province, PR China.
31730027	6	32	theme	gene	621:624	arg1	sequences					626:634	16S rRNA gene sequences	612:634	16S rRNA gene sequences	612:634	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SCAWS-G2T formed a distinct lineage within the order Ktedonobacterales, showing <89.2 % sequence similarities to the recognized taxa of this order.
31730027	1	33	theme	fam	167:169	arg1	nov.					44:47	nov.	44:47	nov.	44:47	nov., a novel representative of the class Ktedonobacteria, isolated from red soil, and proposal of Ktedonosporobacteraceae fam.
31730027	1	33	theme	fam	167:169	arg1	representative					58:71	a novel representative	50:71	a novel representative of the class Ktedonobacteria, isolated from red soil,	50:125	nov., a novel representative of the class Ktedonobacteria, isolated from red soil, and proposal of Ktedonosporobacteraceae fam.
31730027	1	33	theme	fam	167:169	arg1	proposal					131:138	proposal	131:138	proposal of Ktedonosporobacteraceae fam	131:169	nov., a novel representative of the class Ktedonobacteria, isolated from red soil, and proposal of Ktedonosporobacteraceae fam.
31730027	0	34	theme	Ktedonosporobacter	0:17	arg1	nov.					34:37	Ktedonosporobacter rubrisoli gen. nov.	0:37	Ktedonosporobacter rubrisoli gen. nov.	0:37	Ktedonosporobacter rubrisoli gen. nov., sp.
31730027	13	35	theme	rubrisoli	1646:1654	arg1	nov.					1661:1664	Ktedonosporobacter rubrisoli gen. nov.	1627:1664	Ktedonosporobacter rubrisoli gen. nov.	1627:1664	Based on all these data, we propose that strain SCAWS-G2T represents a novel genus and species, Ktedonosporobacter rubrisoli gen. nov., sp.
31730027	13	35	theme	rubrisoli	1646:1654	arg1	genus					1608:1612	a novel genus	1600:1612	a novel genus	1600:1612	Based on all these data, we propose that strain SCAWS-G2T represents a novel genus and species, Ktedonosporobacter rubrisoli gen. nov., sp.
31730027	5	36	theme	rRNA	519:522	arg1	genes					524:528	four 16S rRNA genes	510:528	four 16S rRNA genes with three sequence types (0.4-0.8 % differences)	510:578	The complete genome of strain SCAWS-G2T was a circular chromosome of ~11.34 Mb, which contained four 16S rRNA genes with three sequence types (0.4-0.8 % differences).
31730027	10	37	theme	unidentified	1264:1275	arg1	glycolipids					1277:1287	seven unidentified glycolipids	1258:1287	seven unidentified glycolipids	1258:1287	The polar lipids of SCAWS-G2T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, seven unidentified glycolipids and one unidentified lipid.
31730027	6	38	theme	rRNA	616:619	arg1	sequences					626:634	16S rRNA gene sequences	612:634	16S rRNA gene sequences	612:634	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SCAWS-G2T formed a distinct lineage within the order Ktedonobacterales, showing <89.2 % sequence similarities to the recognized taxa of this order.
31730027	16	39	theme	1.16132T=DSM	1834:1845	arg1	105258T					1847:1853	=CGMCC 1.16132T=DSM 105258T	1827:1853	=CGMCC 1.16132T=DSM 105258T	1827:1853	The type strain of Ktedonosporobacter rubrisoli is SCAWS-G2T (=CGMCC 1.16132T=DSM 105258T).
31730027	16	39	theme	1.16132T=DSM	1834:1845	arg1	SCAWS-G2T					1816:1824	SCAWS-G2T	1816:1824	SCAWS-G2T (=CGMCC 1.16132T=DSM 105258T)	1816:1854	The type strain of Ktedonosporobacter rubrisoli is SCAWS-G2T (=CGMCC 1.16132T=DSM 105258T).
31730027	11	40	theme	amino	1335:1339	arg1	galactose					1441:1449	galactose	1441:1449	galactose	1441:1449	The peptidoglycan amino acids contained ornithine, glycine, glutamic acid and alanine, and the cell-wall sugars were mainly galactose and rhamnose.
31730027	11	40	theme	amino	1335:1339	arg1	acids					1341:1345	The peptidoglycan amino acids	1317:1345	The peptidoglycan amino acids contained ornithine	1317:1365	The peptidoglycan amino acids contained ornithine, glycine, glutamic acid and alanine, and the cell-wall sugars were mainly galactose and rhamnose.
31730027	5	41	with	genes	524:528	arg1	 differences					566:577	0.4-0.8 % differences	557:577	0.4-0.8 % differences	557:577	The complete genome of strain SCAWS-G2T was a circular chromosome of ~11.34 Mb, which contained four 16S rRNA genes with three sequence types (0.4-0.8 % differences).
31730027	5	41	with	genes	524:528	arg1	types					550:554	three sequence types	535:554	three sequence types (0.4-0.8 % differences)	535:578	The complete genome of strain SCAWS-G2T was a circular chromosome of ~11.34 Mb, which contained four 16S rRNA genes with three sequence types (0.4-0.8 % differences).
31730027	6	42	theme	order	702:706	arg1	Ktedonobacterales					708:724	the order Ktedonobacterales	698:724	the order Ktedonobacterales	698:724	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SCAWS-G2T formed a distinct lineage within the order Ktedonobacterales, showing <89.2 % sequence similarities to the recognized taxa of this order.
31730027	1	43	theme	class	80:84	arg1	Ktedonobacteria					86:100	the class Ktedonobacteria	76:100	the class Ktedonobacteria	76:100	nov., a novel representative of the class Ktedonobacteria, isolated from red soil, and proposal of Ktedonosporobacteraceae fam.
31730027	6	44	theme	16S	612:614	arg1	sequences					626:634	16S rRNA gene sequences	612:634	16S rRNA gene sequences	612:634	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SCAWS-G2T formed a distinct lineage within the order Ktedonobacterales, showing <89.2 % sequence similarities to the recognized taxa of this order.
31730027	0	45	theme	gen.	29:32	arg1	nov.					34:37	Ktedonosporobacter rubrisoli gen. nov.	0:37	Ktedonosporobacter rubrisoli gen. nov.	0:37	Ktedonosporobacter rubrisoli gen. nov., sp.
31730027	13	46	theme	novel	1602:1606	arg1	nov.					1661:1664	Ktedonosporobacter rubrisoli gen. nov.	1627:1664	Ktedonosporobacter rubrisoli gen. nov.	1627:1664	Based on all these data, we propose that strain SCAWS-G2T represents a novel genus and species, Ktedonosporobacter rubrisoli gen. nov., sp.
31730027	13	46	theme	novel	1602:1606	arg1	genus					1608:1612	a novel genus	1600:1612	a novel genus	1600:1612	Based on all these data, we propose that strain SCAWS-G2T represents a novel genus and species, Ktedonosporobacter rubrisoli gen. nov., sp.
31730027	13	46	theme	novel	1602:1606	arg1	sp					1667:1668	sp	1667:1668	sp	1667:1668	Based on all these data, we propose that strain SCAWS-G2T represents a novel genus and species, Ktedonosporobacter rubrisoli gen. nov., sp.
31730027	1	47	theme	Ktedonobacteria	86:100	arg1	nov.					44:47	nov.	44:47	nov.	44:47	nov., a novel representative of the class Ktedonobacteria, isolated from red soil, and proposal of Ktedonosporobacteraceae fam.
31730027	1	47	theme	Ktedonobacteria	86:100	arg1	representative					58:71	a novel representative	50:71	a novel representative of the class Ktedonobacteria, isolated from red soil,	50:125	nov., a novel representative of the class Ktedonobacteria, isolated from red soil, and proposal of Ktedonosporobacteraceae fam.
31730027	1	47	theme	Ktedonobacteria	86:100	arg1	proposal					131:138	proposal	131:138	proposal of Ktedonosporobacteraceae fam	131:169	nov., a novel representative of the class Ktedonobacteria, isolated from red soil, and proposal of Ktedonosporobacteraceae fam.
31730027	5	48	theme	complete	418:425	arg1	chromosome					469:478	a circular chromosome	458:478	a circular chromosome	458:478	The complete genome of strain SCAWS-G2T was a circular chromosome of ~11.34 Mb, which contained four 16S rRNA genes with three sequence types (0.4-0.8 % differences).
31730027	5	48	theme	complete	418:425	arg1	genome					427:432	The complete genome	414:432	The complete genome of strain SCAWS-G2T	414:452	The complete genome of strain SCAWS-G2T was a circular chromosome of ~11.34 Mb, which contained four 16S rRNA genes with three sequence types (0.4-0.8 % differences).
31730027	6	49	theme	order	796:800	arg1	taxa					783:786	the recognized taxa	768:786	the recognized taxa of this order	768:800	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SCAWS-G2T formed a distinct lineage within the order Ktedonobacterales, showing <89.2 % sequence similarities to the recognized taxa of this order.
31730027	12	50	theme	fatty	1475:1479	arg1	C16 					1492:1495	C16 	1492:1495	C16 	1492:1495	The major fatty acids were C16 : 1 2-OH, C16 : 0 and iso-C17 : 0.
31730027	12	50	theme	fatty	1475:1479	arg1	acids					1481:1485	The major fatty acids	1465:1485	The major fatty acids	1465:1485	The major fatty acids were C16 : 1 2-OH, C16 : 0 and iso-C17 : 0.
31730027	11	51	theme	glutamic	1377:1384	arg1	acid					1386:1389	glutamic acid	1377:1389	glutamic acid	1377:1389	The peptidoglycan amino acids contained ornithine, glycine, glutamic acid and alanine, and the cell-wall sugars were mainly galactose and rhamnose.
31730027	3	52	attach	isolated	270:277	arg2	bacterium					233:241	A novel filamentous, spore-forming, Gram-stain-positive bacterium	177:241	A novel filamentous, spore-forming, Gram-stain-positive bacterium	177:241	A novel filamentous, spore-forming, Gram-stain-positive bacterium, designated SCAWS-G2T, was isolated from red soil in Jiangxi Province, PR China.
31730027	3	52	attach	isolated	270:277	arg1	soil					288:291	red soil	284:291	red soil in Jiangxi Province, PR China	284:321	A novel filamentous, spore-forming, Gram-stain-positive bacterium, designated SCAWS-G2T, was isolated from red soil in Jiangxi Province, PR China.
31730027	10	53	theme	SCAWS-G2T	1175:1183	arg1	diphosphatidylglycerol					1190:1211	diphosphatidylglycerol	1190:1211	diphosphatidylglycerol	1190:1211	The polar lipids of SCAWS-G2T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, seven unidentified glycolipids and one unidentified lipid.
31730027	10	53	theme	SCAWS-G2T	1175:1183	arg1	lipids					1165:1170	The polar lipids	1155:1170	The polar lipids of SCAWS-G2T	1155:1183	The polar lipids of SCAWS-G2T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, seven unidentified glycolipids and one unidentified lipid.
31730027	14	54	theme	new	1688:1690	arg1	fam					1723:1725	the new family Ktedonosporobacteraceae fam	1684:1725	the new family Ktedonosporobacteraceae fam	1684:1725	nov., within the new family Ktedonosporobacteraceae fam.
31730027	5	55	theme	~11.34 Mb	483:491	arg1	chromosome					469:478	a circular chromosome	458:478	a circular chromosome	458:478	The complete genome of strain SCAWS-G2T was a circular chromosome of ~11.34 Mb, which contained four 16S rRNA genes with three sequence types (0.4-0.8 % differences).
31730027	5	55	theme	~11.34 Mb	483:491	arg1	genome					427:432	The complete genome	414:432	The complete genome of strain SCAWS-G2T	414:452	The complete genome of strain SCAWS-G2T was a circular chromosome of ~11.34 Mb, which contained four 16S rRNA genes with three sequence types (0.4-0.8 % differences).
31730027	6	56	theme	%	741:741	arg1	similarities					752:763	<89.2 % sequence similarities	735:763	<89.2 % sequence similarities to the recognized taxa of this order	735:800	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SCAWS-G2T formed a distinct lineage within the order Ktedonobacterales, showing <89.2 % sequence similarities to the recognized taxa of this order.
31730027	0	57	dep	sp	40:41	arg1	nov.					34:37	Ktedonosporobacter rubrisoli gen. nov.	0:37	Ktedonosporobacter rubrisoli gen. nov.	0:37	Ktedonosporobacter rubrisoli gen. nov., sp.
31730027	9	58	theme	recognized	1092:1101	arg1	families					1103:1110	the recognized families	1088:1110	the recognized families	1088:1110	The strain can also be differentiated from the recognized families by a number of phenotypic characteristics.
31730027	5	59	contain	contained	500:508	arg1	chromosome					469:478	a circular chromosome	458:478	a circular chromosome	458:478	The complete genome of strain SCAWS-G2T was a circular chromosome of ~11.34 Mb, which contained four 16S rRNA genes with three sequence types (0.4-0.8 % differences).
31730027	5	59	contain	contained	500:508	arg1	genome					427:432	The complete genome	414:432	The complete genome of strain SCAWS-G2T	414:452	The complete genome of strain SCAWS-G2T was a circular chromosome of ~11.34 Mb, which contained four 16S rRNA genes with three sequence types (0.4-0.8 % differences).
31730027	5	59	contain	contained	500:508	arg2	genes					524:528	four 16S rRNA genes	510:528	four 16S rRNA genes with three sequence types (0.4-0.8 % differences)	510:578	The complete genome of strain SCAWS-G2T was a circular chromosome of ~11.34 Mb, which contained four 16S rRNA genes with three sequence types (0.4-0.8 % differences).
31730027	16	60	theme	=CGMCC	1827:1832	arg1	105258T					1847:1853	=CGMCC 1.16132T=DSM 105258T	1827:1853	=CGMCC 1.16132T=DSM 105258T	1827:1853	The type strain of Ktedonosporobacter rubrisoli is SCAWS-G2T (=CGMCC 1.16132T=DSM 105258T).
31730027	16	60	theme	=CGMCC	1827:1832	arg1	SCAWS-G2T					1816:1824	SCAWS-G2T	1816:1824	SCAWS-G2T (=CGMCC 1.16132T=DSM 105258T)	1816:1854	The type strain of Ktedonosporobacter rubrisoli is SCAWS-G2T (=CGMCC 1.16132T=DSM 105258T).
31730027	13	61	theme	strain	1572:1577	arg1	SCAWS-G2T					1579:1587	strain SCAWS-G2T	1572:1587	strain SCAWS-G2T	1572:1587	Based on all these data, we propose that strain SCAWS-G2T represents a novel genus and species, Ktedonosporobacter rubrisoli gen. nov., sp.
31730027	16	62	theme	Ktedonosporobacter	1784:1801	arg1	rubrisoli					1803:1811	Ktedonosporobacter rubrisoli	1784:1811	Ktedonosporobacter rubrisoli	1784:1811	The type strain of Ktedonosporobacter rubrisoli is SCAWS-G2T (=CGMCC 1.16132T=DSM 105258T).
31730027	11	63	theme	peptidoglycan	1321:1333	arg1	galactose					1441:1449	galactose	1441:1449	galactose	1441:1449	The peptidoglycan amino acids contained ornithine, glycine, glutamic acid and alanine, and the cell-wall sugars were mainly galactose and rhamnose.
31730027	11	63	theme	peptidoglycan	1321:1333	arg1	acids					1341:1345	The peptidoglycan amino acids	1317:1345	The peptidoglycan amino acids contained ornithine	1317:1365	The peptidoglycan amino acids contained ornithine, glycine, glutamic acid and alanine, and the cell-wall sugars were mainly galactose and rhamnose.
31730027	8	64	theme	type	1018:1021	arg1	strains					1023:1029	the related type strains	1006:1029	the related type strains	1006:1029	The genome-wide average nucleotide identity values between strain SCAWS-G2T and the related type strains were <68.2 %.
31730027	7	65	theme	strain	854:859	arg1	SCAWS-G2T					861:869	strain SCAWS-G2T	854:869	strain SCAWS-G2T	854:869	The whole-genome based phylogenomic tree separated strain SCAWS-G2T from the recognized families within Ktedonobacterales.
31730027	8	66	theme	strain	985:990	arg1	SCAWS-G2T					992:1000	strain SCAWS-G2T	985:1000	strain SCAWS-G2T	985:1000	The genome-wide average nucleotide identity values between strain SCAWS-G2T and the related type strains were <68.2 %.
31730027	6	67	theme	recognized	772:781	arg1	taxa					783:786	the recognized taxa	768:786	the recognized taxa of this order	768:800	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SCAWS-G2T formed a distinct lineage within the order Ktedonobacterales, showing <89.2 % sequence similarities to the recognized taxa of this order.
31730027	8	68	theme	identity	961:968	arg1	values					970:975	The genome-wide average nucleotide identity values	926:975	The genome-wide average nucleotide identity values between strain SCAWS-G2T and the related type strains	926:1029	The genome-wide average nucleotide identity values between strain SCAWS-G2T and the related type strains were <68.2 %.
31730027	8	68	theme	identity	961:968	arg1	%					1042:1042	<68.2 %	1036:1042	<68.2 %	1036:1042	The genome-wide average nucleotide identity values between strain SCAWS-G2T and the related type strains were <68.2 %.
31730027	6	69	theme	distinct	674:681	arg1	lineage					683:689	a distinct lineage	672:689	a distinct lineage	672:689	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SCAWS-G2T formed a distinct lineage within the order Ktedonobacterales, showing <89.2 % sequence similarities to the recognized taxa of this order.
31730027	8	70	theme	nucleotide	950:959	arg1	values					970:975	The genome-wide average nucleotide identity values	926:975	The genome-wide average nucleotide identity values between strain SCAWS-G2T and the related type strains	926:1029	The genome-wide average nucleotide identity values between strain SCAWS-G2T and the related type strains were <68.2 %.
31730027	8	70	theme	nucleotide	950:959	arg1	%					1042:1042	<68.2 %	1036:1042	<68.2 %	1036:1042	The genome-wide average nucleotide identity values between strain SCAWS-G2T and the related type strains were <68.2 %.
31730027	6	71	theme	Phylogenetic	581:592	arg1	analysis					594:601	Phylogenetic analysis	581:601	Phylogenetic analysis based on 16S rRNA gene sequences	581:634	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SCAWS-G2T formed a distinct lineage within the order Ktedonobacterales, showing <89.2 % sequence similarities to the recognized taxa of this order.
31730027	10	72	theme	polar	1159:1163	arg1	diphosphatidylglycerol					1190:1211	diphosphatidylglycerol	1190:1211	diphosphatidylglycerol	1190:1211	The polar lipids of SCAWS-G2T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, seven unidentified glycolipids and one unidentified lipid.
31730027	10	72	theme	polar	1159:1163	arg1	lipids					1165:1170	The polar lipids	1155:1170	The polar lipids of SCAWS-G2T	1155:1183	The polar lipids of SCAWS-G2T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, seven unidentified glycolipids and one unidentified lipid.
31730027	3	73	theme	red	284:286	arg1	soil					288:291	red soil	284:291	red soil in Jiangxi Province, PR China	284:321	A novel filamentous, spore-forming, Gram-stain-positive bacterium, designated SCAWS-G2T, was isolated from red soil in Jiangxi Province, PR China.
31730027	1	74	theme	red	117:119	arg1	soil					121:124	red soil	117:124	red soil	117:124	nov., a novel representative of the class Ktedonobacteria, isolated from red soil, and proposal of Ktedonosporobacteraceae fam.
31730027	14	75	theme	family	1692:1697	arg1	fam					1723:1725	the new family Ktedonosporobacteraceae fam	1684:1725	the new family Ktedonosporobacteraceae fam	1684:1725	nov., within the new family Ktedonosporobacteraceae fam.
31730027	5	76	theme	circular	460:467	arg1	chromosome					469:478	a circular chromosome	458:478	a circular chromosome	458:478	The complete genome of strain SCAWS-G2T was a circular chromosome of ~11.34 Mb, which contained four 16S rRNA genes with three sequence types (0.4-0.8 % differences).
31730027	5	76	theme	circular	460:467	arg1	genome					427:432	The complete genome	414:432	The complete genome of strain SCAWS-G2T	414:452	The complete genome of strain SCAWS-G2T was a circular chromosome of ~11.34 Mb, which contained four 16S rRNA genes with three sequence types (0.4-0.8 % differences).
31730027	16	77	theme	rubrisoli	1803:1811	arg1	strain					1774:1779	The type strain	1765:1779	The type strain of Ktedonosporobacter rubrisoli	1765:1811	The type strain of Ktedonosporobacter rubrisoli is SCAWS-G2T (=CGMCC 1.16132T=DSM 105258T).
31730027	16	77	theme	rubrisoli	1803:1811	arg1	SCAWS-G2T					1816:1824	SCAWS-G2T	1816:1824	SCAWS-G2T (=CGMCC 1.16132T=DSM 105258T)	1816:1854	The type strain of Ktedonosporobacter rubrisoli is SCAWS-G2T (=CGMCC 1.16132T=DSM 105258T).
31730027	3	78	from	soil	288:291	arg1	Province					304:311	Jiangxi Province	296:311	Jiangxi Province	296:311	A novel filamentous, spore-forming, Gram-stain-positive bacterium, designated SCAWS-G2T, was isolated from red soil in Jiangxi Province, PR China.
31730027	3	78	from	soil	288:291	arg1	China					317:321	PR China	314:321	PR China	314:321	A novel filamentous, spore-forming, Gram-stain-positive bacterium, designated SCAWS-G2T, was isolated from red soil in Jiangxi Province, PR China.
31730027	3	79	theme	PR	314:315	arg1	Province					304:311	Jiangxi Province	296:311	Jiangxi Province	296:311	A novel filamentous, spore-forming, Gram-stain-positive bacterium, designated SCAWS-G2T, was isolated from red soil in Jiangxi Province, PR China.
31730027	3	79	theme	PR	314:315	arg1	China					317:321	PR China	314:321	PR China	314:321	A novel filamentous, spore-forming, Gram-stain-positive bacterium, designated SCAWS-G2T, was isolated from red soil in Jiangxi Province, PR China.
31730027	1	80	attach	isolated	103:110	arg2	Ktedonobacteria					86:100	the class Ktedonobacteria	76:100	the class Ktedonobacteria	76:100	nov., a novel representative of the class Ktedonobacteria, isolated from red soil, and proposal of Ktedonosporobacteraceae fam.
31730027	1	80	attach	isolated	103:110	arg1	soil					121:124	red soil	117:124	red soil	117:124	nov., a novel representative of the class Ktedonobacteria, isolated from red soil, and proposal of Ktedonosporobacteraceae fam.
31730027	12	81	dep	C16 	1492:1495	arg1	 0					1511:1512	 0	1511:1512	 0	1511:1512	The major fatty acids were C16 : 1 2-OH, C16 : 0 and iso-C17 : 0.
31730027	12	81	dep	C16 	1492:1495	arg1	C16 					1506:1509	C16 	1506:1509	C16 	1506:1509	The major fatty acids were C16 : 1 2-OH, C16 : 0 and iso-C17 : 0.
31730027	12	81	dep	C16 	1492:1495	arg1	 0					1527:1528	 0	1527:1528	C16 : 1 2-OH, C16 : 0 and iso-C17 : 0	1492:1528	The major fatty acids were C16 : 1 2-OH, C16 : 0 and iso-C17 : 0.
31730027	12	81	dep	C16 	1492:1495	arg1	iso-C17 					1518:1525	iso-C17 	1518:1525	iso-C17 	1518:1525	The major fatty acids were C16 : 1 2-OH, C16 : 0 and iso-C17 : 0.
31730027	3	82	dep	filamentous	185:195	arg1	spore-forming					198:210	spore-forming	198:210	spore-forming	198:210	A novel filamentous, spore-forming, Gram-stain-positive bacterium, designated SCAWS-G2T, was isolated from red soil in Jiangxi Province, PR China.
31730027	3	82	dep	filamentous	185:195	arg1	Gram-stain-positive					213:231	Gram-stain-positive	213:231	Gram-stain-positive	213:231	A novel filamentous, spore-forming, Gram-stain-positive bacterium, designated SCAWS-G2T, was isolated from red soil in Jiangxi Province, PR China.
31730027	7	83	theme	based	820:824	arg1	tree					839:842	The whole-genome based phylogenomic tree	803:842	The whole-genome based phylogenomic tree	803:842	The whole-genome based phylogenomic tree separated strain SCAWS-G2T from the recognized families within Ktedonobacterales.
35126378	0	0	theme	plantarum	89:97	arg1	Strains					99:105	Lactobacillus plantarum Strains	75:105	Lactobacillus plantarum Strains	75:105	Effect of D-Ala-Ended Peptidoglycan Precursors on the Immune Regulation of Lactobacillus plantarum Strains.
35126378	4	1	theme	lactis	758:763	arg1	strains					695:701	several strains	687:701	several strains of L. plantarum expressing the ddl gene of Lactococcus lactis	687:763	To study the effect of peptidoglycan precursors on immunity, we constructed several strains of L. plantarum expressing the ddl gene of Lactococcus lactis to change their peptidoglycan precursors.
35126378	4	2	theme	peptidoglycan	634:646	arg1	precursors					648:657	peptidoglycan precursors	634:657	peptidoglycan precursors	634:657	To study the effect of peptidoglycan precursors on immunity, we constructed several strains of L. plantarum expressing the ddl gene of Lactococcus lactis to change their peptidoglycan precursors.
35126378	0	3	theme	Lactobacillus	75:87	arg1	Strains					99:105	Lactobacillus plantarum Strains	75:105	Lactobacillus plantarum Strains	75:105	Effect of D-Ala-Ended Peptidoglycan Precursors on the Immune Regulation of Lactobacillus plantarum Strains.
35126378	7	4	from	increase	1224:1231	arg1	precursors					1269:1278	D-Ala L. plantarum peptidoglycan precursors	1236:1278	D-Ala L. plantarum peptidoglycan precursors	1236:1278	We further explored the regulation of the macrophage inflammatory response pathway by the wild-type and constructed strains, and found that these strains induced the MyD88-dependent TRAF6/MAPK pathway, and the increase in D-Ala L. plantarum peptidoglycan precursors increased the secretion of the inflammatory factors IL-6, IL-1β and TNF-α.
35126378	4	5	theme	Lactococcus	746:756	arg1	lactis					758:763	Lactococcus lactis	746:763	Lactococcus lactis	746:763	To study the effect of peptidoglycan precursors on immunity, we constructed several strains of L. plantarum expressing the ddl gene of Lactococcus lactis to change their peptidoglycan precursors.
35126378	0	6	from	Effect	0:5	arg1	Regulation					61:70	the Immune Regulation	50:70	the Immune Regulation of Lactobacillus plantarum Strains	50:105	Effect of D-Ala-Ended Peptidoglycan Precursors on the Immune Regulation of Lactobacillus plantarum Strains.
35126378	7	7	theme	MyD88-dependent	1180:1194	arg1	pathway					1207:1213	the MyD88-dependent TRAF6/MAPK pathway	1176:1213	the MyD88-dependent TRAF6/MAPK pathway	1176:1213	We further explored the regulation of the macrophage inflammatory response pathway by the wild-type and constructed strains, and found that these strains induced the MyD88-dependent TRAF6/MAPK pathway, and the increase in D-Ala L. plantarum peptidoglycan precursors increased the secretion of the inflammatory factors IL-6, IL-1β and TNF-α.
35126378	0	8	theme	Strains	99:105	arg1	Regulation					61:70	the Immune Regulation	50:70	the Immune Regulation of Lactobacillus plantarum Strains	50:105	Effect of D-Ala-Ended Peptidoglycan Precursors on the Immune Regulation of Lactobacillus plantarum Strains.
35126378	7	9	theme	TRAF6/MAPK	1196:1205	arg1	pathway					1207:1213	the MyD88-dependent TRAF6/MAPK pathway	1176:1213	the MyD88-dependent TRAF6/MAPK pathway	1176:1213	We further explored the regulation of the macrophage inflammatory response pathway by the wild-type and constructed strains, and found that these strains induced the MyD88-dependent TRAF6/MAPK pathway, and the increase in D-Ala L. plantarum peptidoglycan precursors increased the secretion of the inflammatory factors IL-6, IL-1β and TNF-α.
35126378	8	10	theme	central	1427:1433	arg1	role					1435:1438	a central role	1425:1438	a central role	1425:1438	These results indicate that D-Ala-ended peptidoglycan precursors play a central role in the variable immunomodulatory ability of L. plantarum.
35126378	4	11	theme	precursors	648:657	arg1	effect					624:629	the effect	620:629	the effect of peptidoglycan precursors on immunity	620:669	To study the effect of peptidoglycan precursors on immunity, we constructed several strains of L. plantarum expressing the ddl gene of Lactococcus lactis to change their peptidoglycan precursors.
35126378	8	12	theme	variable	1447:1454	arg1	ability					1473:1479	the variable immunomodulatory ability	1443:1479	the variable immunomodulatory ability of L. plantarum	1443:1495	These results indicate that D-Ala-ended peptidoglycan precursors play a central role in the variable immunomodulatory ability of L. plantarum.
35126378	1	13	theme	peptidoglycan	179:191	arg1	composition					193:203	its peptidoglycan composition	175:203	its peptidoglycan composition	175:203	The resistance of Lactobacillus plantarum to vancomycin depends on its peptidoglycan composition.
35126378	6	14	theme	ddl	951:953	arg1	overexpression					933:946	The overexpression	929:946	The overexpression of ddl	929:953	The overexpression of ddl increased the susceptibility of the strains to vancomycin.
35126378	4	15	theme	plantarum	709:717	arg1	strains					695:701	several strains	687:701	several strains of L. plantarum expressing the ddl gene of Lactococcus lactis	687:763	To study the effect of peptidoglycan precursors on immunity, we constructed several strains of L. plantarum expressing the ddl gene of Lactococcus lactis to change their peptidoglycan precursors.
35126378	4	16	from	effect	624:629	arg1	immunity					662:669	immunity	662:669	immunity	662:669	To study the effect of peptidoglycan precursors on immunity, we constructed several strains of L. plantarum expressing the ddl gene of Lactococcus lactis to change their peptidoglycan precursors.
35126378	7	17	theme	plantarum	1245:1253	arg1	precursors					1269:1278	D-Ala L. plantarum peptidoglycan precursors	1236:1278	D-Ala L. plantarum peptidoglycan precursors	1236:1278	We further explored the regulation of the macrophage inflammatory response pathway by the wild-type and constructed strains, and found that these strains induced the MyD88-dependent TRAF6/MAPK pathway, and the increase in D-Ala L. plantarum peptidoglycan precursors increased the secretion of the inflammatory factors IL-6, IL-1β and TNF-α.
35126378	4	18	theme	ddl	734:736	arg1	gene					738:741	the ddl gene	730:741	the ddl gene	730:741	To study the effect of peptidoglycan precursors on immunity, we constructed several strains of L. plantarum expressing the ddl gene of Lactococcus lactis to change their peptidoglycan precursors.
35126378	7	19	theme	peptidoglycan	1255:1267	arg1	precursors					1269:1278	D-Ala L. plantarum peptidoglycan precursors	1236:1278	D-Ala L. plantarum peptidoglycan precursors	1236:1278	We further explored the regulation of the macrophage inflammatory response pathway by the wild-type and constructed strains, and found that these strains induced the MyD88-dependent TRAF6/MAPK pathway, and the increase in D-Ala L. plantarum peptidoglycan precursors increased the secretion of the inflammatory factors IL-6, IL-1β and TNF-α.
35126378	8	20	theme	immunomodulatory	1456:1471	arg1	ability					1473:1479	the variable immunomodulatory ability	1443:1479	the variable immunomodulatory ability of L. plantarum	1443:1495	These results indicate that D-Ala-ended peptidoglycan precursors play a central role in the variable immunomodulatory ability of L. plantarum.
35126378	0	21	theme	Peptidoglycan	22:34	arg1	Precursors					36:45	D-Ala-Ended Peptidoglycan Precursors	10:45	D-Ala-Ended Peptidoglycan Precursors	10:45	Effect of D-Ala-Ended Peptidoglycan Precursors on the Immune Regulation of Lactobacillus plantarum Strains.
35126378	8	22	theme	peptidoglycan	1395:1407	arg1	precursors					1409:1418	D-Ala-ended peptidoglycan precursors	1383:1418	D-Ala-ended peptidoglycan precursors	1383:1418	These results indicate that D-Ala-ended peptidoglycan precursors play a central role in the variable immunomodulatory ability of L. plantarum.
35126378	2	23	theme	peptidoglycan	338:350	arg1	precursors					352:361	peptidoglycan precursors	338:361	peptidoglycan precursors ending in D-alanyl-D-alanine (D-Ala-D-Ala)	338:404	Vancomycin has poor binding affinity with peptidoglycan precursors ending in D-alanyl-D-lactate (D-Ala-D-Lac) but binds strongly to peptidoglycan precursors ending in D-alanyl-D-alanine (D-Ala-D-Ala), resulting in resistance and sensitivity, respectively.
35126378	2	24	contain	has	217:219	arg2	affinity					234:241	poor binding affinity	221:241	poor binding affinity	221:241	Vancomycin has poor binding affinity with peptidoglycan precursors ending in D-alanyl-D-lactate (D-Ala-D-Lac) but binds strongly to peptidoglycan precursors ending in D-alanyl-D-alanine (D-Ala-D-Ala), resulting in resistance and sensitivity, respectively.
35126378	2	24	contain	has	217:219	arg1	Vancomycin					206:215	Vancomycin	206:215	Vancomycin	206:215	Vancomycin has poor binding affinity with peptidoglycan precursors ending in D-alanyl-D-lactate (D-Ala-D-Lac) but binds strongly to peptidoglycan precursors ending in D-alanyl-D-alanine (D-Ala-D-Ala), resulting in resistance and sensitivity, respectively.
35126378	0	25	theme	D-Ala-Ended	10:20	arg1	Precursors					36:45	D-Ala-Ended Peptidoglycan Precursors	10:45	D-Ala-Ended Peptidoglycan Precursors	10:45	Effect of D-Ala-Ended Peptidoglycan Precursors on the Immune Regulation of Lactobacillus plantarum Strains.
35126378	8	26	theme	plantarum	1487:1495	arg1	ability					1473:1479	the variable immunomodulatory ability	1443:1479	the variable immunomodulatory ability of L. plantarum	1443:1495	These results indicate that D-Ala-ended peptidoglycan precursors play a central role in the variable immunomodulatory ability of L. plantarum.
35126378	4	27	theme	several	687:693	arg1	strains					695:701	several strains	687:701	several strains of L. plantarum expressing the ddl gene of Lactococcus lactis	687:763	To study the effect of peptidoglycan precursors on immunity, we constructed several strains of L. plantarum expressing the ddl gene of Lactococcus lactis to change their peptidoglycan precursors.
35126378	5	28	from	change	811:816	arg1	termini					825:831	the termini	821:831	the termini of the peptidoglycan precursors	821:863	The change in the termini of the peptidoglycan precursors was determined by the sensitivity of the strains to vancomycin.
35126378	5	29	theme	strains	906:912	arg1	sensitivity					887:897	the sensitivity	883:897	the sensitivity of the strains to vancomycin	883:926	The change in the termini of the peptidoglycan precursors was determined by the sensitivity of the strains to vancomycin.
35126378	0	30	theme	Precursors	36:45	arg1	Effect					0:5	Effect	0:5	Effect of D-Ala-Ended Peptidoglycan Precursors on the Immune Regulation of Lactobacillus plantarum Strains.	0:106	Effect of D-Ala-Ended Peptidoglycan Precursors on the Immune Regulation of Lactobacillus plantarum Strains.
35126378	3	31	theme	ligase	466:471	arg1	Ddl					473:475	The ligase Ddl	462:475	The ligase Ddl	462:475	The ligase Ddl, which generates D-Ala-D-Lac or D-Ala-D-Ala incorporated into the peptidoglycan precursor chain, is responsible for this specificity.
35126378	3	31	theme	ligase	466:471	arg1	responsible					577:587	responsible	577:587	responsible	577:587	The ligase Ddl, which generates D-Ala-D-Lac or D-Ala-D-Ala incorporated into the peptidoglycan precursor chain, is responsible for this specificity.
35126378	7	32	theme	D-Ala	1236:1240	arg1	precursors					1269:1278	D-Ala L. plantarum peptidoglycan precursors	1236:1278	D-Ala L. plantarum peptidoglycan precursors	1236:1278	We further explored the regulation of the macrophage inflammatory response pathway by the wild-type and constructed strains, and found that these strains induced the MyD88-dependent TRAF6/MAPK pathway, and the increase in D-Ala L. plantarum peptidoglycan precursors increased the secretion of the inflammatory factors IL-6, IL-1β and TNF-α.
35126378	0	33	theme	Immune	54:59	arg1	Regulation					61:70	the Immune Regulation	50:70	the Immune Regulation of Lactobacillus plantarum Strains	50:105	Effect of D-Ala-Ended Peptidoglycan Precursors on the Immune Regulation of Lactobacillus plantarum Strains.
35126378	7	34	theme	constructed	1118:1128	arg1	strains					1130:1136	the wild-type and constructed strains	1100:1136	the wild-type and constructed strains	1100:1136	We further explored the regulation of the macrophage inflammatory response pathway by the wild-type and constructed strains, and found that these strains induced the MyD88-dependent TRAF6/MAPK pathway, and the increase in D-Ala L. plantarum peptidoglycan precursors increased the secretion of the inflammatory factors IL-6, IL-1β and TNF-α.
35126378	7	35	theme	factors	1324:1330	arg1	secretion					1294:1302	the secretion	1290:1302	the secretion of the inflammatory factors IL-6, IL-1β and TNF-α	1290:1352	We further explored the regulation of the macrophage inflammatory response pathway by the wild-type and constructed strains, and found that these strains induced the MyD88-dependent TRAF6/MAPK pathway, and the increase in D-Ala L. plantarum peptidoglycan precursors increased the secretion of the inflammatory factors IL-6, IL-1β and TNF-α.
35126378	2	36	theme	peptidoglycan	248:260	arg1	precursors					262:271	peptidoglycan precursors	248:271	peptidoglycan precursors ending in D-alanyl-D-lactate (D-Ala-D-Lac)	248:314	Vancomycin has poor binding affinity with peptidoglycan precursors ending in D-alanyl-D-lactate (D-Ala-D-Lac) but binds strongly to peptidoglycan precursors ending in D-alanyl-D-alanine (D-Ala-D-Ala), resulting in resistance and sensitivity, respectively.
35126378	3	37	theme	peptidoglycan	543:555	arg1	chain					567:571	the peptidoglycan precursor chain	539:571	the peptidoglycan precursor chain	539:571	The ligase Ddl, which generates D-Ala-D-Lac or D-Ala-D-Ala incorporated into the peptidoglycan precursor chain, is responsible for this specificity.
35126378	7	38	dep	factors	1324:1330	arg1	IL-1β					1338:1342	IL-1β	1338:1342	IL-1β	1338:1342	We further explored the regulation of the macrophage inflammatory response pathway by the wild-type and constructed strains, and found that these strains induced the MyD88-dependent TRAF6/MAPK pathway, and the increase in D-Ala L. plantarum peptidoglycan precursors increased the secretion of the inflammatory factors IL-6, IL-1β and TNF-α.
35126378	7	38	dep	factors	1324:1330	arg1	IL-6					1332:1335	IL-6	1332:1335	IL-6	1332:1335	We further explored the regulation of the macrophage inflammatory response pathway by the wild-type and constructed strains, and found that these strains induced the MyD88-dependent TRAF6/MAPK pathway, and the increase in D-Ala L. plantarum peptidoglycan precursors increased the secretion of the inflammatory factors IL-6, IL-1β and TNF-α.
35126378	7	38	dep	factors	1324:1330	arg1	TNF-α					1348:1352	TNF-α	1348:1352	TNF-α	1348:1352	We further explored the regulation of the macrophage inflammatory response pathway by the wild-type and constructed strains, and found that these strains induced the MyD88-dependent TRAF6/MAPK pathway, and the increase in D-Ala L. plantarum peptidoglycan precursors increased the secretion of the inflammatory factors IL-6, IL-1β and TNF-α.
35126378	7	38	dep	factors	1324:1330	arg1	factors					1324:1330	the inflammatory factors IL-6, IL-1β and TNF-α	1307:1352	the inflammatory factors IL-6, IL-1β and TNF-α	1307:1352	We further explored the regulation of the macrophage inflammatory response pathway by the wild-type and constructed strains, and found that these strains induced the MyD88-dependent TRAF6/MAPK pathway, and the increase in D-Ala L. plantarum peptidoglycan precursors increased the secretion of the inflammatory factors IL-6, IL-1β and TNF-α.
35126378	7	39	theme	response	1080:1087	arg1	pathway					1089:1095	the macrophage inflammatory response pathway	1052:1095	the macrophage inflammatory response pathway	1052:1095	We further explored the regulation of the macrophage inflammatory response pathway by the wild-type and constructed strains, and found that these strains induced the MyD88-dependent TRAF6/MAPK pathway, and the increase in D-Ala L. plantarum peptidoglycan precursors increased the secretion of the inflammatory factors IL-6, IL-1β and TNF-α.
35126378	3	40	theme	precursor	557:565	arg1	chain					567:571	the peptidoglycan precursor chain	539:571	the peptidoglycan precursor chain	539:571	The ligase Ddl, which generates D-Ala-D-Lac or D-Ala-D-Ala incorporated into the peptidoglycan precursor chain, is responsible for this specificity.
35126378	7	41	theme	wild-type	1104:1112	arg1	strains					1130:1136	the wild-type and constructed strains	1100:1136	the wild-type and constructed strains	1100:1136	We further explored the regulation of the macrophage inflammatory response pathway by the wild-type and constructed strains, and found that these strains induced the MyD88-dependent TRAF6/MAPK pathway, and the increase in D-Ala L. plantarum peptidoglycan precursors increased the secretion of the inflammatory factors IL-6, IL-1β and TNF-α.
35126378	2	42	theme	binding	226:232	arg1	affinity					234:241	poor binding affinity	221:241	poor binding affinity	221:241	Vancomycin has poor binding affinity with peptidoglycan precursors ending in D-alanyl-D-lactate (D-Ala-D-Lac) but binds strongly to peptidoglycan precursors ending in D-alanyl-D-alanine (D-Ala-D-Ala), resulting in resistance and sensitivity, respectively.
35126378	7	43	theme	pathway	1089:1095	arg1	regulation					1038:1047	the regulation	1034:1047	the regulation of the macrophage inflammatory response pathway by the wild-type and constructed strains	1034:1136	We further explored the regulation of the macrophage inflammatory response pathway by the wild-type and constructed strains, and found that these strains induced the MyD88-dependent TRAF6/MAPK pathway, and the increase in D-Ala L. plantarum peptidoglycan precursors increased the secretion of the inflammatory factors IL-6, IL-1β and TNF-α.
35126378	1	44	theme	plantarum	140:148	arg1	resistance					112:121	The resistance	108:121	The resistance of Lactobacillus plantarum to vancomycin	108:162	The resistance of Lactobacillus plantarum to vancomycin depends on its peptidoglycan composition.
35126378	2	45	theme	poor	221:224	arg1	affinity					234:241	poor binding affinity	221:241	poor binding affinity	221:241	Vancomycin has poor binding affinity with peptidoglycan precursors ending in D-alanyl-D-lactate (D-Ala-D-Lac) but binds strongly to peptidoglycan precursors ending in D-alanyl-D-alanine (D-Ala-D-Ala), resulting in resistance and sensitivity, respectively.
35126378	7	46	theme	macrophage	1056:1065	arg1	pathway					1089:1095	the macrophage inflammatory response pathway	1052:1095	the macrophage inflammatory response pathway	1052:1095	We further explored the regulation of the macrophage inflammatory response pathway by the wild-type and constructed strains, and found that these strains induced the MyD88-dependent TRAF6/MAPK pathway, and the increase in D-Ala L. plantarum peptidoglycan precursors increased the secretion of the inflammatory factors IL-6, IL-1β and TNF-α.
35126378	8	47	theme	D-Ala-ended	1383:1393	arg1	precursors					1409:1418	D-Ala-ended peptidoglycan precursors	1383:1418	D-Ala-ended peptidoglycan precursors	1383:1418	These results indicate that D-Ala-ended peptidoglycan precursors play a central role in the variable immunomodulatory ability of L. plantarum.
35126378	4	48	theme	peptidoglycan	781:793	arg1	precursors					795:804	their peptidoglycan precursors	775:804	their peptidoglycan precursors	775:804	To study the effect of peptidoglycan precursors on immunity, we constructed several strains of L. plantarum expressing the ddl gene of Lactococcus lactis to change their peptidoglycan precursors.
35126378	7	49	theme	inflammatory	1311:1322	arg1	IL-1β					1338:1342	IL-1β	1338:1342	IL-1β	1338:1342	We further explored the regulation of the macrophage inflammatory response pathway by the wild-type and constructed strains, and found that these strains induced the MyD88-dependent TRAF6/MAPK pathway, and the increase in D-Ala L. plantarum peptidoglycan precursors increased the secretion of the inflammatory factors IL-6, IL-1β and TNF-α.
35126378	7	49	theme	inflammatory	1311:1322	arg1	IL-6					1332:1335	IL-6	1332:1335	IL-6	1332:1335	We further explored the regulation of the macrophage inflammatory response pathway by the wild-type and constructed strains, and found that these strains induced the MyD88-dependent TRAF6/MAPK pathway, and the increase in D-Ala L. plantarum peptidoglycan precursors increased the secretion of the inflammatory factors IL-6, IL-1β and TNF-α.
35126378	7	49	theme	inflammatory	1311:1322	arg1	TNF-α					1348:1352	TNF-α	1348:1352	TNF-α	1348:1352	We further explored the regulation of the macrophage inflammatory response pathway by the wild-type and constructed strains, and found that these strains induced the MyD88-dependent TRAF6/MAPK pathway, and the increase in D-Ala L. plantarum peptidoglycan precursors increased the secretion of the inflammatory factors IL-6, IL-1β and TNF-α.
35126378	7	49	theme	inflammatory	1311:1322	arg1	factors					1324:1330	the inflammatory factors IL-6, IL-1β and TNF-α	1307:1352	the inflammatory factors IL-6, IL-1β and TNF-α	1307:1352	We further explored the regulation of the macrophage inflammatory response pathway by the wild-type and constructed strains, and found that these strains induced the MyD88-dependent TRAF6/MAPK pathway, and the increase in D-Ala L. plantarum peptidoglycan precursors increased the secretion of the inflammatory factors IL-6, IL-1β and TNF-α.
35126378	7	50	theme	inflammatory	1067:1078	arg1	pathway					1089:1095	the macrophage inflammatory response pathway	1052:1095	the macrophage inflammatory response pathway	1052:1095	We further explored the regulation of the macrophage inflammatory response pathway by the wild-type and constructed strains, and found that these strains induced the MyD88-dependent TRAF6/MAPK pathway, and the increase in D-Ala L. plantarum peptidoglycan precursors increased the secretion of the inflammatory factors IL-6, IL-1β and TNF-α.
35126378	5	51	theme	precursors	854:863	arg1	termini					825:831	the termini	821:831	the termini of the peptidoglycan precursors	821:863	The change in the termini of the peptidoglycan precursors was determined by the sensitivity of the strains to vancomycin.
35126378	6	52	theme	strains	991:997	arg1	susceptibility					969:982	the susceptibility	965:982	the susceptibility of the strains to vancomycin	965:1011	The overexpression of ddl increased the susceptibility of the strains to vancomycin.
35126378	5	53	theme	peptidoglycan	840:852	arg1	precursors					854:863	the peptidoglycan precursors	836:863	the peptidoglycan precursors	836:863	The change in the termini of the peptidoglycan precursors was determined by the sensitivity of the strains to vancomycin.
35126378	7	54	theme	L.	1242:1243	arg1	precursors					1269:1278	D-Ala L. plantarum peptidoglycan precursors	1236:1278	D-Ala L. plantarum peptidoglycan precursors	1236:1278	We further explored the regulation of the macrophage inflammatory response pathway by the wild-type and constructed strains, and found that these strains induced the MyD88-dependent TRAF6/MAPK pathway, and the increase in D-Ala L. plantarum peptidoglycan precursors increased the secretion of the inflammatory factors IL-6, IL-1β and TNF-α.
34735562	8	0	theme	compound	1314:1321	arg1	delivery					1323:1330	hydrophobic bioactive compound delivery	1292:1330	hydrophobic bioactive compound delivery	1292:1330	In summary, these results demonstrated that the food-grade cod protein-chitosan nanocomplex had potential in the development of HIPPEs, which could be used as carriers for hydrophobic bioactive compound delivery.
34735562	3	1	theme	internal	521:528	arg1	phase					530:534	an internal phase	518:534	an internal phase as high as 84%	518:549	The application of the cod protein-chitosan nanocomplex was demonstrated in the formation of stable HIPPEs with an internal phase as high as 84%.
34735562	4	2	theme	composition	580:590	arg1	influence					556:564	The influence	552:564	The influence of the system composition on the stability, microstructure and rheology of the HIPPEs	552:650	The influence of the system composition on the stability, microstructure and rheology of the HIPPEs was determined.
34735562	8	3	theme	protein-chitosan	1183:1198	arg1	nanocomplex					1200:1210	the food-grade cod protein-chitosan nanocomplex	1164:1210	the food-grade cod protein-chitosan nanocomplex	1164:1210	In summary, these results demonstrated that the food-grade cod protein-chitosan nanocomplex had potential in the development of HIPPEs, which could be used as carriers for hydrophobic bioactive compound delivery.
34735562	7	4	theme	astaxanthin	999:1009	arg1	w/w					1093:1095	w/w	1093:1095	w/w	1093:1095	The bioavailability of astaxanthin in the HIPPEs stabilized by the nanocomplexes of 2.0% (w/w) cod protein and 0.1% (w/w) chitosan reached 49%.
34735562	7	4	theme	astaxanthin	999:1009	arg1	%					1090:1090	0.1%	1087:1090	0.1% (w/w)	1087:1096	The bioavailability of astaxanthin in the HIPPEs stabilized by the nanocomplexes of 2.0% (w/w) cod protein and 0.1% (w/w) chitosan reached 49%.
34735562	7	4	theme	astaxanthin	999:1009	arg1	astaxanthin					999:1009	astaxanthin	999:1009	astaxanthin	999:1009	The bioavailability of astaxanthin in the HIPPEs stabilized by the nanocomplexes of 2.0% (w/w) cod protein and 0.1% (w/w) chitosan reached 49%.
34735562	7	4	theme	astaxanthin	999:1009	arg1	bioavailability					980:994	The bioavailability	976:994	The bioavailability of astaxanthin in the HIPPEs stabilized by the nanocomplexes of 2.0% (w/w) cod protein	976:1081	The bioavailability of astaxanthin in the HIPPEs stabilized by the nanocomplexes of 2.0% (w/w) cod protein and 0.1% (w/w) chitosan reached 49%.
34735562	7	5	theme	protein	1075:1081	arg1	nanocomplexes					1043:1055	the nanocomplexes	1039:1055	the nanocomplexes of 2.0% (w/w) cod protein	1039:1081	The bioavailability of astaxanthin in the HIPPEs stabilized by the nanocomplexes of 2.0% (w/w) cod protein and 0.1% (w/w) chitosan reached 49%.
34735562	4	6	theme	system	573:578	arg1	composition					580:590	the system composition	569:590	the system composition	569:590	The influence of the system composition on the stability, microstructure and rheology of the HIPPEs was determined.
34735562	7	7	dep	%	1063:1063	arg1	w/w					1066:1068	w/w	1066:1068	w/w	1066:1068	The bioavailability of astaxanthin in the HIPPEs stabilized by the nanocomplexes of 2.0% (w/w) cod protein and 0.1% (w/w) chitosan reached 49%.
34735562	0	8	theme	astaxanthin	93:103	arg1	delivery					105:112	astaxanthin delivery	93:112	astaxanthin delivery	93:112	High internal phase Pickering emulsions stabilized by a cod protein-chitosan nanocomplex for astaxanthin delivery.
34735562	5	9	theme	three-dimensional	747:763	arg1	structure					773:781	a compact three-dimensional network structure	737:781	a compact three-dimensional network structure	737:781	The HIPPEs stabilized by the cod protein-chitosan nanocomplex formed a compact three-dimensional network structure, which gave the emulsion a higher storage modulus, viscoelasticity and good thixotropy.
34735562	2	10	theme	particle	348:355	arg1	nanocomplex					274:284	a novel cod protein-chitosan nanocomplex	245:284	a novel cod protein-chitosan nanocomplex	245:284	In this study, a novel cod protein-chitosan nanocomplex was prepared through electrostatic interactions and used as a particle emulsifier to stabilize the oil-water interface.
34735562	2	10	theme	particle	348:355	arg1	emulsifier					357:366	a particle emulsifier	346:366	a particle emulsifier to stabilize the oil-water interface	346:403	In this study, a novel cod protein-chitosan nanocomplex was prepared through electrostatic interactions and used as a particle emulsifier to stabilize the oil-water interface.
34735562	5	11	theme	storage	817:823	arg1	thixotropy					859:868	good thixotropy	854:868	good thixotropy	854:868	The HIPPEs stabilized by the cod protein-chitosan nanocomplex formed a compact three-dimensional network structure, which gave the emulsion a higher storage modulus, viscoelasticity and good thixotropy.
34735562	5	11	theme	storage	817:823	arg1	modulus					825:831	the emulsion a higher storage modulus	795:831	the emulsion a higher storage modulus	795:831	The HIPPEs stabilized by the cod protein-chitosan nanocomplex formed a compact three-dimensional network structure, which gave the emulsion a higher storage modulus, viscoelasticity and good thixotropy.
34735562	5	11	theme	storage	817:823	arg1	viscoelasticity					834:848	viscoelasticity	834:848	viscoelasticity	834:848	The HIPPEs stabilized by the cod protein-chitosan nanocomplex formed a compact three-dimensional network structure, which gave the emulsion a higher storage modulus, viscoelasticity and good thixotropy.
34735562	1	12	theme	food	180:183	arg1	protein					185:191	a food protein	178:191	a food protein	178:191	High internal phase Pickering emulsions (HIPPEs) stabilized by a food protein have attracted widespread attention.
34735562	5	13	theme	network	765:771	arg1	structure					773:781	a compact three-dimensional network structure	737:781	a compact three-dimensional network structure	737:781	The HIPPEs stabilized by the cod protein-chitosan nanocomplex formed a compact three-dimensional network structure, which gave the emulsion a higher storage modulus, viscoelasticity and good thixotropy.
34735562	3	14	with	HIPPEs	506:511	arg1	phase					530:534	an internal phase	518:534	an internal phase as high as 84%	518:549	The application of the cod protein-chitosan nanocomplex was demonstrated in the formation of stable HIPPEs with an internal phase as high as 84%.
34735562	2	15	used	used	338:341	arg2	emulsifier					357:366	a particle emulsifier	346:366	a particle emulsifier to stabilize the oil-water interface	346:403	In this study, a novel cod protein-chitosan nanocomplex was prepared through electrostatic interactions and used as a particle emulsifier to stabilize the oil-water interface.
34735562	2	15	used	used	338:341	arg2	nanocomplex					274:284	a novel cod protein-chitosan nanocomplex	245:284	a novel cod protein-chitosan nanocomplex	245:284	In this study, a novel cod protein-chitosan nanocomplex was prepared through electrostatic interactions and used as a particle emulsifier to stabilize the oil-water interface.
34735562	2	16	theme	protein-chitosan	257:272	arg1	nanocomplex					274:284	a novel cod protein-chitosan nanocomplex	245:284	a novel cod protein-chitosan nanocomplex	245:284	In this study, a novel cod protein-chitosan nanocomplex was prepared through electrostatic interactions and used as a particle emulsifier to stabilize the oil-water interface.
34735562	2	16	theme	protein-chitosan	257:272	arg1	emulsifier					357:366	a particle emulsifier	346:366	a particle emulsifier to stabilize the oil-water interface	346:403	In this study, a novel cod protein-chitosan nanocomplex was prepared through electrostatic interactions and used as a particle emulsifier to stabilize the oil-water interface.
34735562	0	17	theme	internal	5:12	arg1	emulsions					30:38	High internal phase Pickering emulsions	0:38	High internal phase Pickering emulsions	0:38	High internal phase Pickering emulsions stabilized by a cod protein-chitosan nanocomplex for astaxanthin delivery.
34735562	6	18	theme	astaxanthin	912:922	arg1	stability					899:907	the chemical stability	886:907	the chemical stability of astaxanthin	886:922	Interestingly, the chemical stability of astaxanthin was significantly improved by the developed HIPPEs.
34735562	3	19	theme	protein-chitosan	433:448	arg1	nanocomplex					450:460	the cod protein-chitosan nanocomplex	425:460	the cod protein-chitosan nanocomplex	425:460	The application of the cod protein-chitosan nanocomplex was demonstrated in the formation of stable HIPPEs with an internal phase as high as 84%.
34735562	0	20	theme	High	0:3	arg1	emulsions					30:38	High internal phase Pickering emulsions	0:38	High internal phase Pickering emulsions	0:38	High internal phase Pickering emulsions stabilized by a cod protein-chitosan nanocomplex for astaxanthin delivery.
34735562	7	21	from	%	1090:1090	arg1	HIPPEs					1018:1023	the HIPPEs	1014:1023	the HIPPEs stabilized by the nanocomplexes of 2.0% (w/w) cod protein	1014:1081	The bioavailability of astaxanthin in the HIPPEs stabilized by the nanocomplexes of 2.0% (w/w) cod protein and 0.1% (w/w) chitosan reached 49%.
34735562	2	22	theme	oil-water	385:393	arg1	interface					395:403	the oil-water interface	381:403	the oil-water interface	381:403	In this study, a novel cod protein-chitosan nanocomplex was prepared through electrostatic interactions and used as a particle emulsifier to stabilize the oil-water interface.
34735562	0	23	theme	Pickering	20:28	arg1	emulsions					30:38	High internal phase Pickering emulsions	0:38	High internal phase Pickering emulsions	0:38	High internal phase Pickering emulsions stabilized by a cod protein-chitosan nanocomplex for astaxanthin delivery.
34735562	5	24	theme	cod	697:699	arg1	nanocomplex					718:728	the cod protein-chitosan nanocomplex	693:728	the cod protein-chitosan nanocomplex	693:728	The HIPPEs stabilized by the cod protein-chitosan nanocomplex formed a compact three-dimensional network structure, which gave the emulsion a higher storage modulus, viscoelasticity and good thixotropy.
34735562	2	25	theme	electrostatic	307:319	arg1	interactions					321:332	electrostatic interactions	307:332	electrostatic interactions	307:332	In this study, a novel cod protein-chitosan nanocomplex was prepared through electrostatic interactions and used as a particle emulsifier to stabilize the oil-water interface.
34735562	0	26	theme	phase	14:18	arg1	emulsions					30:38	High internal phase Pickering emulsions	0:38	High internal phase Pickering emulsions	0:38	High internal phase Pickering emulsions stabilized by a cod protein-chitosan nanocomplex for astaxanthin delivery.
34735562	5	27	theme	protein-chitosan	701:716	arg1	nanocomplex					718:728	the cod protein-chitosan nanocomplex	693:728	the cod protein-chitosan nanocomplex	693:728	The HIPPEs stabilized by the cod protein-chitosan nanocomplex formed a compact three-dimensional network structure, which gave the emulsion a higher storage modulus, viscoelasticity and good thixotropy.
34735562	6	28	theme	chemical	890:897	arg1	stability					899:907	the chemical stability	886:907	the chemical stability of astaxanthin	886:922	Interestingly, the chemical stability of astaxanthin was significantly improved by the developed HIPPEs.
34735562	1	29	theme	High	115:118	arg1	HIPPEs					156:161	HIPPEs	156:161	HIPPEs	156:161	High internal phase Pickering emulsions (HIPPEs) stabilized by a food protein have attracted widespread attention.
34735562	1	29	theme	High	115:118	arg1	emulsions					145:153	High internal phase Pickering emulsions	115:153	High internal phase Pickering emulsions (HIPPEs) stabilized by a food protein	115:191	High internal phase Pickering emulsions (HIPPEs) stabilized by a food protein have attracted widespread attention.
34735562	5	30	theme	good	854:857	arg1	thixotropy					859:868	good thixotropy	854:868	good thixotropy	854:868	The HIPPEs stabilized by the cod protein-chitosan nanocomplex formed a compact three-dimensional network structure, which gave the emulsion a higher storage modulus, viscoelasticity and good thixotropy.
34735562	5	30	theme	good	854:857	arg1	modulus					825:831	the emulsion a higher storage modulus	795:831	the emulsion a higher storage modulus	795:831	The HIPPEs stabilized by the cod protein-chitosan nanocomplex formed a compact three-dimensional network structure, which gave the emulsion a higher storage modulus, viscoelasticity and good thixotropy.
34735562	7	31	theme	cod	1071:1073	arg1	protein					1075:1081	2.0% (w/w) cod protein	1060:1081	2.0% (w/w) cod protein	1060:1081	The bioavailability of astaxanthin in the HIPPEs stabilized by the nanocomplexes of 2.0% (w/w) cod protein and 0.1% (w/w) chitosan reached 49%.
34735562	4	32	theme	HIPPEs	645:650	arg1	rheology					629:636	rheology	629:636	rheology	629:636	The influence of the system composition on the stability, microstructure and rheology of the HIPPEs was determined.
34735562	4	32	theme	HIPPEs	645:650	arg1	microstructure					610:623	microstructure	610:623	microstructure	610:623	The influence of the system composition on the stability, microstructure and rheology of the HIPPEs was determined.
34735562	4	32	theme	HIPPEs	645:650	arg1	stability					599:607	stability	599:607	stability	599:607	The influence of the system composition on the stability, microstructure and rheology of the HIPPEs was determined.
34735562	8	33	theme	bioactive	1304:1312	arg1	delivery					1323:1330	hydrophobic bioactive compound delivery	1292:1330	hydrophobic bioactive compound delivery	1292:1330	In summary, these results demonstrated that the food-grade cod protein-chitosan nanocomplex had potential in the development of HIPPEs, which could be used as carriers for hydrophobic bioactive compound delivery.
34735562	3	34	theme	nanocomplex	450:460	arg1	application					410:420	The application	406:420	The application of the cod protein-chitosan nanocomplex	406:460	The application of the cod protein-chitosan nanocomplex was demonstrated in the formation of stable HIPPEs with an internal phase as high as 84%.
34735562	1	35	theme	widespread	208:217	arg1	attention					219:227	widespread attention	208:227	widespread attention	208:227	High internal phase Pickering emulsions (HIPPEs) stabilized by a food protein have attracted widespread attention.
34735562	3	36	theme	cod	429:431	arg1	nanocomplex					450:460	the cod protein-chitosan nanocomplex	425:460	the cod protein-chitosan nanocomplex	425:460	The application of the cod protein-chitosan nanocomplex was demonstrated in the formation of stable HIPPEs with an internal phase as high as 84%.
34735562	5	37	theme	emulsion	799:806	arg1	a					808:808	emulsion a	799:808	emulsion a	799:808	The HIPPEs stabilized by the cod protein-chitosan nanocomplex formed a compact three-dimensional network structure, which gave the emulsion a higher storage modulus, viscoelasticity and good thixotropy.
34735562	8	38	theme	hydrophobic	1292:1302	arg1	delivery					1323:1330	hydrophobic bioactive compound delivery	1292:1330	hydrophobic bioactive compound delivery	1292:1330	In summary, these results demonstrated that the food-grade cod protein-chitosan nanocomplex had potential in the development of HIPPEs, which could be used as carriers for hydrophobic bioactive compound delivery.
34735562	4	39	from	influence	556:564	arg1	rheology					629:636	rheology	629:636	rheology	629:636	The influence of the system composition on the stability, microstructure and rheology of the HIPPEs was determined.
34735562	4	39	from	influence	556:564	arg1	microstructure					610:623	microstructure	610:623	microstructure	610:623	The influence of the system composition on the stability, microstructure and rheology of the HIPPEs was determined.
34735562	4	39	from	influence	556:564	arg1	stability					599:607	stability	599:607	stability	599:607	The influence of the system composition on the stability, microstructure and rheology of the HIPPEs was determined.
34735562	8	40	theme	food-grade	1168:1177	arg1	nanocomplex					1200:1210	the food-grade cod protein-chitosan nanocomplex	1164:1210	the food-grade cod protein-chitosan nanocomplex	1164:1210	In summary, these results demonstrated that the food-grade cod protein-chitosan nanocomplex had potential in the development of HIPPEs, which could be used as carriers for hydrophobic bioactive compound delivery.
34735562	8	41	used	used	1271:1274	arg2	HIPPEs					1248:1253	HIPPEs	1248:1253	HIPPEs	1248:1253	In summary, these results demonstrated that the food-grade cod protein-chitosan nanocomplex had potential in the development of HIPPEs, which could be used as carriers for hydrophobic bioactive compound delivery.
34735562	8	41	used	used	1271:1274	arg2	carriers					1279:1286	carriers	1279:1286	carriers for hydrophobic bioactive compound delivery	1279:1330	In summary, these results demonstrated that the food-grade cod protein-chitosan nanocomplex had potential in the development of HIPPEs, which could be used as carriers for hydrophobic bioactive compound delivery.
34735562	7	42	theme	2.0	1060:1062	arg1	%					1063:1063	%	1063:1063	%	1063:1063	The bioavailability of astaxanthin in the HIPPEs stabilized by the nanocomplexes of 2.0% (w/w) cod protein and 0.1% (w/w) chitosan reached 49%.
34735562	1	43	theme	internal	120:127	arg1	HIPPEs					156:161	HIPPEs	156:161	HIPPEs	156:161	High internal phase Pickering emulsions (HIPPEs) stabilized by a food protein have attracted widespread attention.
34735562	1	43	theme	internal	120:127	arg1	emulsions					145:153	High internal phase Pickering emulsions	115:153	High internal phase Pickering emulsions (HIPPEs) stabilized by a food protein	115:191	High internal phase Pickering emulsions (HIPPEs) stabilized by a food protein have attracted widespread attention.
34735562	5	44	theme	higher	810:815	arg1	thixotropy					859:868	good thixotropy	854:868	good thixotropy	854:868	The HIPPEs stabilized by the cod protein-chitosan nanocomplex formed a compact three-dimensional network structure, which gave the emulsion a higher storage modulus, viscoelasticity and good thixotropy.
34735562	5	44	theme	higher	810:815	arg1	modulus					825:831	the emulsion a higher storage modulus	795:831	the emulsion a higher storage modulus	795:831	The HIPPEs stabilized by the cod protein-chitosan nanocomplex formed a compact three-dimensional network structure, which gave the emulsion a higher storage modulus, viscoelasticity and good thixotropy.
34735562	5	44	theme	higher	810:815	arg1	viscoelasticity					834:848	viscoelasticity	834:848	viscoelasticity	834:848	The HIPPEs stabilized by the cod protein-chitosan nanocomplex formed a compact three-dimensional network structure, which gave the emulsion a higher storage modulus, viscoelasticity and good thixotropy.
34735562	8	45	contain	had	1212:1214	arg2	potential					1216:1224	potential	1216:1224	potential	1216:1224	In summary, these results demonstrated that the food-grade cod protein-chitosan nanocomplex had potential in the development of HIPPEs, which could be used as carriers for hydrophobic bioactive compound delivery.
34735562	8	45	contain	had	1212:1214	arg1	nanocomplex					1200:1210	the food-grade cod protein-chitosan nanocomplex	1164:1210	the food-grade cod protein-chitosan nanocomplex	1164:1210	In summary, these results demonstrated that the food-grade cod protein-chitosan nanocomplex had potential in the development of HIPPEs, which could be used as carriers for hydrophobic bioactive compound delivery.
34735562	1	46	theme	phase	129:133	arg1	HIPPEs					156:161	HIPPEs	156:161	HIPPEs	156:161	High internal phase Pickering emulsions (HIPPEs) stabilized by a food protein have attracted widespread attention.
34735562	1	46	theme	phase	129:133	arg1	emulsions					145:153	High internal phase Pickering emulsions	115:153	High internal phase Pickering emulsions (HIPPEs) stabilized by a food protein	115:191	High internal phase Pickering emulsions (HIPPEs) stabilized by a food protein have attracted widespread attention.
34735562	7	47	dep	bioavailability	980:994	arg1	chitosan					1098:1105	chitosan	1098:1105	chitosan	1098:1105	The bioavailability of astaxanthin in the HIPPEs stabilized by the nanocomplexes of 2.0% (w/w) cod protein and 0.1% (w/w) chitosan reached 49%.
34735562	6	48	theme	developed	958:966	arg1	HIPPEs					968:973	the developed HIPPEs	954:973	the developed HIPPEs	954:973	Interestingly, the chemical stability of astaxanthin was significantly improved by the developed HIPPEs.
34735562	7	49	theme	%	1063:1063	arg1	protein					1075:1081	2.0% (w/w) cod protein	1060:1081	2.0% (w/w) cod protein	1060:1081	The bioavailability of astaxanthin in the HIPPEs stabilized by the nanocomplexes of 2.0% (w/w) cod protein and 0.1% (w/w) chitosan reached 49%.
34735562	2	50	theme	cod	253:255	arg1	nanocomplex					274:284	a novel cod protein-chitosan nanocomplex	245:284	a novel cod protein-chitosan nanocomplex	245:284	In this study, a novel cod protein-chitosan nanocomplex was prepared through electrostatic interactions and used as a particle emulsifier to stabilize the oil-water interface.
34735562	2	50	theme	cod	253:255	arg1	emulsifier					357:366	a particle emulsifier	346:366	a particle emulsifier to stabilize the oil-water interface	346:403	In this study, a novel cod protein-chitosan nanocomplex was prepared through electrostatic interactions and used as a particle emulsifier to stabilize the oil-water interface.
34735562	8	51	theme	HIPPEs	1248:1253	arg1	development					1233:1243	the development	1229:1243	the development of HIPPEs, which could be used as carriers for hydrophobic bioactive compound delivery	1229:1330	In summary, these results demonstrated that the food-grade cod protein-chitosan nanocomplex had potential in the development of HIPPEs, which could be used as carriers for hydrophobic bioactive compound delivery.
34735562	1	52	theme	Pickering	135:143	arg1	HIPPEs					156:161	HIPPEs	156:161	HIPPEs	156:161	High internal phase Pickering emulsions (HIPPEs) stabilized by a food protein have attracted widespread attention.
34735562	1	52	theme	Pickering	135:143	arg1	emulsions					145:153	High internal phase Pickering emulsions	115:153	High internal phase Pickering emulsions (HIPPEs) stabilized by a food protein	115:191	High internal phase Pickering emulsions (HIPPEs) stabilized by a food protein have attracted widespread attention.
34735562	2	53	theme	novel	247:251	arg1	nanocomplex					274:284	a novel cod protein-chitosan nanocomplex	245:284	a novel cod protein-chitosan nanocomplex	245:284	In this study, a novel cod protein-chitosan nanocomplex was prepared through electrostatic interactions and used as a particle emulsifier to stabilize the oil-water interface.
34735562	2	53	theme	novel	247:251	arg1	emulsifier					357:366	a particle emulsifier	346:366	a particle emulsifier to stabilize the oil-water interface	346:403	In this study, a novel cod protein-chitosan nanocomplex was prepared through electrostatic interactions and used as a particle emulsifier to stabilize the oil-water interface.
34735562	3	54	theme	HIPPEs	506:511	arg1	formation					486:494	the formation	482:494	the formation of stable HIPPEs with an internal phase as high as 84%	482:549	The application of the cod protein-chitosan nanocomplex was demonstrated in the formation of stable HIPPEs with an internal phase as high as 84%.
34735562	3	55	theme	stable	499:504	arg1	HIPPEs					506:511	stable HIPPEs	499:511	stable HIPPEs with an internal phase as high as 84%	499:549	The application of the cod protein-chitosan nanocomplex was demonstrated in the formation of stable HIPPEs with an internal phase as high as 84%.
34735562	0	56	theme	protein-chitosan	60:75	arg1	nanocomplex					77:87	a cod protein-chitosan nanocomplex	54:87	a cod protein-chitosan nanocomplex for astaxanthin delivery	54:112	High internal phase Pickering emulsions stabilized by a cod protein-chitosan nanocomplex for astaxanthin delivery.
34735562	7	57	from	bioavailability	980:994	arg1	HIPPEs					1018:1023	the HIPPEs	1014:1023	the HIPPEs stabilized by the nanocomplexes of 2.0% (w/w) cod protein	1014:1081	The bioavailability of astaxanthin in the HIPPEs stabilized by the nanocomplexes of 2.0% (w/w) cod protein and 0.1% (w/w) chitosan reached 49%.
34735562	0	58	theme	cod	56:58	arg1	nanocomplex					77:87	a cod protein-chitosan nanocomplex	54:87	a cod protein-chitosan nanocomplex for astaxanthin delivery	54:112	High internal phase Pickering emulsions stabilized by a cod protein-chitosan nanocomplex for astaxanthin delivery.
34735562	8	59	theme	cod	1179:1181	arg1	nanocomplex					1200:1210	the food-grade cod protein-chitosan nanocomplex	1164:1210	the food-grade cod protein-chitosan nanocomplex	1164:1210	In summary, these results demonstrated that the food-grade cod protein-chitosan nanocomplex had potential in the development of HIPPEs, which could be used as carriers for hydrophobic bioactive compound delivery.
34735562	3	60	theme	high	539:542	arg1	phase					530:534	an internal phase	518:534	an internal phase as high as 84%	518:549	The application of the cod protein-chitosan nanocomplex was demonstrated in the formation of stable HIPPEs with an internal phase as high as 84%.
34735562	5	61	theme	compact	739:745	arg1	structure					773:781	a compact three-dimensional network structure	737:781	a compact three-dimensional network structure	737:781	The HIPPEs stabilized by the cod protein-chitosan nanocomplex formed a compact three-dimensional network structure, which gave the emulsion a higher storage modulus, viscoelasticity and good thixotropy.
33171183	2	0	theme	silver	502:507	arg1	chitosan					534:541	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan	445:541	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers	445:597	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	3	1	theme	good	698:701	arg1	properties					714:723	good mechanical properties	698:723	good mechanical properties	698:723	The present dressings exhibited high swelling, suitable water vapor transmission rate (WVTR), and good mechanical properties and degradability, as well as sustained release of PRP.
33171183	4	2	theme	excellent	850:858	arg1	performance					874:884	excellent antibacterial performance	850:884	excellent antibacterial performance	850:884	Besides, the component of Ag@CMC-PAMAM nanoparticles endow them with excellent antibacterial performance, while the incorporation of PRP promotes the effect of anti-inflammatory and angiogenesis by up-regulating relative activity factor expression of TGF-β1, CD31 and α-SMA and down-regulating the inflammatory-relative genes including TNF-α, IL-6 and IL-1β, all of which promote the closure of wound and produce a superior healing effect to the commercial Aquacel Ag group.
33171183	1	3	theme	bacterial	172:180	arg1	strains					213:219	bacterial even multi-resistant bacterial strains	172:219	bacterial even multi-resistant bacterial strains	172:219	Nowadays, treatment to the infected wounds caused by bacterial even multi-resistant bacterial strains and subsequently complete skin regeneration remain a critical clinical challenge.
33171183	4	4	theme	α-SMA	1049:1053	arg1	expression					1018:1027	relative activity factor expression	993:1027	relative activity factor expression of TGF-β1, CD31 and α-SMA	993:1053	Besides, the component of Ag@CMC-PAMAM nanoparticles endow them with excellent antibacterial performance, while the incorporation of PRP promotes the effect of anti-inflammatory and angiogenesis by up-regulating relative activity factor expression of TGF-β1, CD31 and α-SMA and down-regulating the inflammatory-relative genes including TNF-α, IL-6 and IL-1β, all of which promote the closure of wound and produce a superior healing effect to the commercial Aquacel Ag group.
33171183	0	5	theme	wound	102:106	arg1	treatment					108:116	wound treatment	102:116	wound treatment	102:116	Development of nanosilver doped carboxymethyl chitosan-polyamideamine alginate composite dressing for wound treatment.
33171183	4	6	theme	angiogenesis	963:974	arg1	effect					931:936	the effect	927:936	the effect of anti-inflammatory and angiogenesis	927:974	Besides, the component of Ag@CMC-PAMAM nanoparticles endow them with excellent antibacterial performance, while the incorporation of PRP promotes the effect of anti-inflammatory and angiogenesis by up-regulating relative activity factor expression of TGF-β1, CD31 and α-SMA and down-regulating the inflammatory-relative genes including TNF-α, IL-6 and IL-1β, all of which promote the closure of wound and produce a superior healing effect to the commercial Aquacel Ag group.
33171183	2	7	theme	wound	387:391	arg1	dressings					393:401	wound dressings	387:401	wound dressings	387:401	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	2	7	theme	wound	387:391	arg1	platform					336:343	a novel multi-functional platform	311:343	a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP)	311:369	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	2	8	theme	chitosan	534:541	arg1	CMC-PAMAM					570:578	Ag@CMC-PAMAM	567:578	Ag@CMC-PAMAM	567:578	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	2	8	theme	chitosan	534:541	arg1	polyamideamine					551:564	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine	445:564	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers	445:597	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	5	9	theme	healing	1420:1426	arg1	effect					1377:1382	synergistic effect	1365:1382	synergistic effect of antibacterial property and wound healing	1365:1426	This work indicates that the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing is a promising biomaterial with synergistic effect of antibacterial property and wound healing.
33171183	5	10	with	biomaterial	1348:1358	arg1	effect					1377:1382	synergistic effect	1365:1382	synergistic effect of antibacterial property and wound healing	1365:1426	This work indicates that the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing is a promising biomaterial with synergistic effect of antibacterial property and wound healing.
33171183	2	11	theme	carboxymethyl	520:532	arg1	chitosan					534:541	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan	445:541	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers	445:597	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	4	12	theme	antibacterial	860:872	arg1	performance					874:884	excellent antibacterial performance	850:884	excellent antibacterial performance	850:884	Besides, the component of Ag@CMC-PAMAM nanoparticles endow them with excellent antibacterial performance, while the incorporation of PRP promotes the effect of anti-inflammatory and angiogenesis by up-regulating relative activity factor expression of TGF-β1, CD31 and α-SMA and down-regulating the inflammatory-relative genes including TNF-α, IL-6 and IL-1β, all of which promote the closure of wound and produce a superior healing effect to the commercial Aquacel Ag group.
33171183	4	13	theme	Ag	1246:1247	arg1	group					1249:1253	the commercial Aquacel Ag group	1223:1253	the commercial Aquacel Ag group	1223:1253	Besides, the component of Ag@CMC-PAMAM nanoparticles endow them with excellent antibacterial performance, while the incorporation of PRP promotes the effect of anti-inflammatory and angiogenesis by up-regulating relative activity factor expression of TGF-β1, CD31 and α-SMA and down-regulating the inflammatory-relative genes including TNF-α, IL-6 and IL-1β, all of which promote the closure of wound and produce a superior healing effect to the commercial Aquacel Ag group.
33171183	2	14	theme	multi-functional	319:334	arg1	dressings					393:401	wound dressings	387:401	wound dressings	387:401	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	2	14	theme	multi-functional	319:334	arg1	platform					336:343	a novel multi-functional platform	311:343	a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP)	311:369	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	2	14	theme	multi-functional	319:334	arg1	CMC-PAMAM/PRP					356:368	Alg/1.0Ag@CMC-PAMAM/PRP	346:368	Alg/1.0Ag@CMC-PAMAM/PRP	346:368	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	4	15	theme	activity	1002:1009	arg1	expression					1018:1027	relative activity factor expression	993:1027	relative activity factor expression of TGF-β1, CD31 and α-SMA	993:1053	Besides, the component of Ag@CMC-PAMAM nanoparticles endow them with excellent antibacterial performance, while the incorporation of PRP promotes the effect of anti-inflammatory and angiogenesis by up-regulating relative activity factor expression of TGF-β1, CD31 and α-SMA and down-regulating the inflammatory-relative genes including TNF-α, IL-6 and IL-1β, all of which promote the closure of wound and produce a superior healing effect to the commercial Aquacel Ag group.
33171183	2	16	theme	-doped	513:518	arg1	chitosan					534:541	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan	445:541	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers	445:597	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	4	17	theme	Aquacel	1238:1244	arg1	group					1249:1253	the commercial Aquacel Ag group	1223:1253	the commercial Aquacel Ag group	1223:1253	Besides, the component of Ag@CMC-PAMAM nanoparticles endow them with excellent antibacterial performance, while the incorporation of PRP promotes the effect of anti-inflammatory and angiogenesis by up-regulating relative activity factor expression of TGF-β1, CD31 and α-SMA and down-regulating the inflammatory-relative genes including TNF-α, IL-6 and IL-1β, all of which promote the closure of wound and produce a superior healing effect to the commercial Aquacel Ag group.
33171183	2	18	theme	novel	313:317	arg1	dressings					393:401	wound dressings	387:401	wound dressings	387:401	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	2	18	theme	novel	313:317	arg1	platform					336:343	a novel multi-functional platform	311:343	a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP)	311:369	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	2	18	theme	novel	313:317	arg1	CMC-PAMAM/PRP					356:368	Alg/1.0Ag@CMC-PAMAM/PRP	346:368	Alg/1.0Ag@CMC-PAMAM/PRP	346:368	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	1	19	theme	multi-resistant	187:201	arg1	strains					213:219	bacterial even multi-resistant bacterial strains	172:219	bacterial even multi-resistant bacterial strains	172:219	Nowadays, treatment to the infected wounds caused by bacterial even multi-resistant bacterial strains and subsequently complete skin regeneration remain a critical clinical challenge.
33171183	4	20	theme	anti-inflammatory	941:957	arg1	effect					931:936	the effect	927:936	the effect of anti-inflammatory and angiogenesis	927:974	Besides, the component of Ag@CMC-PAMAM nanoparticles endow them with excellent antibacterial performance, while the incorporation of PRP promotes the effect of anti-inflammatory and angiogenesis by up-regulating relative activity factor expression of TGF-β1, CD31 and α-SMA and down-regulating the inflammatory-relative genes including TNF-α, IL-6 and IL-1β, all of which promote the closure of wound and produce a superior healing effect to the commercial Aquacel Ag group.
33171183	3	21	theme	mechanical	703:712	arg1	properties					714:723	good mechanical properties	698:723	good mechanical properties	698:723	The present dressings exhibited high swelling, suitable water vapor transmission rate (WVTR), and good mechanical properties and degradability, as well as sustained release of PRP.
33171183	2	22	theme	containing	486:495	arg1	chitosan					534:541	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan	445:541	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers	445:597	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	4	23	theme	healing	1205:1211	arg1	effect					1213:1218	a superior healing effect	1194:1218	a superior healing effect to the commercial Aquacel Ag group	1194:1253	Besides, the component of Ag@CMC-PAMAM nanoparticles endow them with excellent antibacterial performance, while the incorporation of PRP promotes the effect of anti-inflammatory and angiogenesis by up-regulating relative activity factor expression of TGF-β1, CD31 and α-SMA and down-regulating the inflammatory-relative genes including TNF-α, IL-6 and IL-1β, all of which promote the closure of wound and produce a superior healing effect to the commercial Aquacel Ag group.
33171183	1	24	theme	bacterial	203:211	arg1	strains					213:219	bacterial even multi-resistant bacterial strains	172:219	bacterial even multi-resistant bacterial strains	172:219	Nowadays, treatment to the infected wounds caused by bacterial even multi-resistant bacterial strains and subsequently complete skin regeneration remain a critical clinical challenge.
33171183	2	25	theme	Alg	466:468	arg1	chitosan					534:541	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan	445:541	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers	445:597	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	2	26	theme	nano	497:500	arg1	chitosan					534:541	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan	445:541	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers	445:597	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	4	27	theme	superior	1196:1203	arg1	effect					1213:1218	a superior healing effect	1194:1218	a superior healing effect to the commercial Aquacel Ag group	1194:1253	Besides, the component of Ag@CMC-PAMAM nanoparticles endow them with excellent antibacterial performance, while the incorporation of PRP promotes the effect of anti-inflammatory and angiogenesis by up-regulating relative activity factor expression of TGF-β1, CD31 and α-SMA and down-regulating the inflammatory-relative genes including TNF-α, IL-6 and IL-1β, all of which promote the closure of wound and produce a superior healing effect to the commercial Aquacel Ag group.
33171183	5	28	theme	prepared	1285:1292	arg1	biomaterial					1348:1358	a promising biomaterial	1336:1358	a promising biomaterial with synergistic effect of antibacterial property and wound healing	1336:1426	This work indicates that the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing is a promising biomaterial with synergistic effect of antibacterial property and wound healing.
33171183	5	28	theme	prepared	1285:1292	arg1	dressing					1324:1331	the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing	1281:1331	the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing	1281:1331	This work indicates that the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing is a promising biomaterial with synergistic effect of antibacterial property and wound healing.
33171183	5	29	theme	promising	1338:1346	arg1	biomaterial					1348:1358	a promising biomaterial	1336:1358	a promising biomaterial with synergistic effect of antibacterial property and wound healing	1336:1426	This work indicates that the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing is a promising biomaterial with synergistic effect of antibacterial property and wound healing.
33171183	5	29	theme	promising	1338:1346	arg1	dressing					1324:1331	the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing	1281:1331	the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing	1281:1331	This work indicates that the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing is a promising biomaterial with synergistic effect of antibacterial property and wound healing.
33171183	2	30	theme	rich	422:425	arg1	plasma					427:432	platelet rich plasma	413:432	platelet rich plasma (PRP)	413:438	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	2	30	theme	rich	422:425	arg1	PRP					435:437	PRP	435:437	PRP	435:437	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	4	31	theme	TGF-β1	1032:1037	arg1	expression					1018:1027	relative activity factor expression	993:1027	relative activity factor expression of TGF-β1, CD31 and α-SMA	993:1053	Besides, the component of Ag@CMC-PAMAM nanoparticles endow them with excellent antibacterial performance, while the incorporation of PRP promotes the effect of anti-inflammatory and angiogenesis by up-regulating relative activity factor expression of TGF-β1, CD31 and α-SMA and down-regulating the inflammatory-relative genes including TNF-α, IL-6 and IL-1β, all of which promote the closure of wound and produce a superior healing effect to the commercial Aquacel Ag group.
33171183	2	32	theme	@	355:355	arg1	platform					336:343	a novel multi-functional platform	311:343	a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP)	311:369	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	2	32	theme	@	355:355	arg1	CMC-PAMAM/PRP					356:368	Alg/1.0Ag@CMC-PAMAM/PRP	346:368	Alg/1.0Ag@CMC-PAMAM/PRP	346:368	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	5	33	theme	Alg/1.0Ag	1294:1302	arg1	biomaterial					1348:1358	a promising biomaterial	1336:1358	a promising biomaterial with synergistic effect of antibacterial property and wound healing	1336:1426	This work indicates that the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing is a promising biomaterial with synergistic effect of antibacterial property and wound healing.
33171183	5	33	theme	Alg/1.0Ag	1294:1302	arg1	dressing					1324:1331	the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing	1281:1331	the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing	1281:1331	This work indicates that the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing is a promising biomaterial with synergistic effect of antibacterial property and wound healing.
33171183	2	34	theme	platelet	413:420	arg1	plasma					427:432	platelet rich plasma	413:432	platelet rich plasma (PRP)	413:438	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	2	34	theme	platelet	413:420	arg1	PRP					435:437	PRP	435:437	PRP	435:437	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	2	35	theme	alginate	456:463	arg1	chitosan					534:541	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan	445:541	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers	445:597	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	2	36	theme	Ag	567:568	arg1	CMC-PAMAM					570:578	Ag@CMC-PAMAM	567:578	Ag@CMC-PAMAM	567:578	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	2	36	theme	Ag	567:568	arg1	polyamideamine					551:564	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine	445:564	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers	445:597	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	4	37	theme	factor	1011:1016	arg1	expression					1018:1027	relative activity factor expression	993:1027	relative activity factor expression of TGF-β1, CD31 and α-SMA	993:1053	Besides, the component of Ag@CMC-PAMAM nanoparticles endow them with excellent antibacterial performance, while the incorporation of PRP promotes the effect of anti-inflammatory and angiogenesis by up-regulating relative activity factor expression of TGF-β1, CD31 and α-SMA and down-regulating the inflammatory-relative genes including TNF-α, IL-6 and IL-1β, all of which promote the closure of wound and produce a superior healing effect to the commercial Aquacel Ag group.
33171183	2	38	theme	Alg/1.0Ag	346:354	arg1	platform					336:343	a novel multi-functional platform	311:343	a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP)	311:369	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	2	38	theme	Alg/1.0Ag	346:354	arg1	CMC-PAMAM/PRP					356:368	Alg/1.0Ag@CMC-PAMAM/PRP	346:368	Alg/1.0Ag@CMC-PAMAM/PRP	346:368	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	4	39	theme	commercial	1227:1236	arg1	group					1249:1253	the commercial Aquacel Ag group	1223:1253	the commercial Aquacel Ag group	1223:1253	Besides, the component of Ag@CMC-PAMAM nanoparticles endow them with excellent antibacterial performance, while the incorporation of PRP promotes the effect of anti-inflammatory and angiogenesis by up-regulating relative activity factor expression of TGF-β1, CD31 and α-SMA and down-regulating the inflammatory-relative genes including TNF-α, IL-6 and IL-1β, all of which promote the closure of wound and produce a superior healing effect to the commercial Aquacel Ag group.
33171183	0	40	theme	nanosilver	15:24	arg1	Development					0:10	Development	0:10	Development of nanosilver	0:24	Development of nanosilver doped carboxymethyl chitosan-polyamideamine alginate composite dressing for wound treatment.
33171183	3	41	theme	water	656:660	arg1	rate					681:684	suitable water vapor transmission rate	647:684	suitable water vapor transmission rate (WVTR)	647:691	The present dressings exhibited high swelling, suitable water vapor transmission rate (WVTR), and good mechanical properties and degradability, as well as sustained release of PRP.
33171183	3	41	theme	water	656:660	arg1	WVTR					687:690	WVTR	687:690	WVTR	687:690	The present dressings exhibited high swelling, suitable water vapor transmission rate (WVTR), and good mechanical properties and degradability, as well as sustained release of PRP.
33171183	5	42	theme	wound	1414:1418	arg1	healing					1420:1426	wound healing	1414:1426	wound healing	1414:1426	This work indicates that the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing is a promising biomaterial with synergistic effect of antibacterial property and wound healing.
33171183	5	43	theme	CMC-PAMAM/PRP	1304:1316	arg1	biomaterial					1348:1358	a promising biomaterial	1336:1358	a promising biomaterial with synergistic effect of antibacterial property and wound healing	1336:1426	This work indicates that the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing is a promising biomaterial with synergistic effect of antibacterial property and wound healing.
33171183	5	43	theme	CMC-PAMAM/PRP	1304:1316	arg1	dressing					1324:1331	the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing	1281:1331	the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing	1281:1331	This work indicates that the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing is a promising biomaterial with synergistic effect of antibacterial property and wound healing.
33171183	1	44	theme	complete	238:245	arg1	regeneration					252:263	subsequently complete skin regeneration	225:263	subsequently complete skin regeneration	225:263	Nowadays, treatment to the infected wounds caused by bacterial even multi-resistant bacterial strains and subsequently complete skin regeneration remain a critical clinical challenge.
33171183	0	45	theme	chitosan-polyamideamine	46:68	arg1	dressing					89:96	carboxymethyl chitosan-polyamideamine alginate composite dressing	32:96	carboxymethyl chitosan-polyamideamine alginate composite dressing for wound treatment	32:116	Development of nanosilver doped carboxymethyl chitosan-polyamideamine alginate composite dressing for wound treatment.
33171183	2	46	theme	polyamideamine	551:564	arg1	polymers					590:597	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers	445:597	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers	445:597	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	3	47	theme	vapor	662:666	arg1	rate					681:684	suitable water vapor transmission rate	647:684	suitable water vapor transmission rate (WVTR)	647:691	The present dressings exhibited high swelling, suitable water vapor transmission rate (WVTR), and good mechanical properties and degradability, as well as sustained release of PRP.
33171183	3	47	theme	vapor	662:666	arg1	WVTR					687:690	WVTR	687:690	WVTR	687:690	The present dressings exhibited high swelling, suitable water vapor transmission rate (WVTR), and good mechanical properties and degradability, as well as sustained release of PRP.
33171183	5	48	theme	antibacterial	1387:1399	arg1	property					1401:1408	antibacterial property	1387:1408	antibacterial property	1387:1408	This work indicates that the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing is a promising biomaterial with synergistic effect of antibacterial property and wound healing.
33171183	5	49	theme	wound	1318:1322	arg1	biomaterial					1348:1358	a promising biomaterial	1336:1358	a promising biomaterial with synergistic effect of antibacterial property and wound healing	1336:1426	This work indicates that the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing is a promising biomaterial with synergistic effect of antibacterial property and wound healing.
33171183	5	49	theme	wound	1318:1322	arg1	dressing					1324:1331	the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing	1281:1331	the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing	1281:1331	This work indicates that the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing is a promising biomaterial with synergistic effect of antibacterial property and wound healing.
33171183	1	50	theme	skin	247:250	arg1	regeneration					252:263	subsequently complete skin regeneration	225:263	subsequently complete skin regeneration	225:263	Nowadays, treatment to the infected wounds caused by bacterial even multi-resistant bacterial strains and subsequently complete skin regeneration remain a critical clinical challenge.
33171183	0	51	theme	carboxymethyl	32:44	arg1	dressing					89:96	carboxymethyl chitosan-polyamideamine alginate composite dressing	32:96	carboxymethyl chitosan-polyamideamine alginate composite dressing for wound treatment	32:116	Development of nanosilver doped carboxymethyl chitosan-polyamideamine alginate composite dressing for wound treatment.
33171183	2	52	theme	dressing	477:484	arg1	chitosan					534:541	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan	445:541	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers	445:597	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	3	53	theme	present	604:610	arg1	dressings					612:620	The present dressings	600:620	The present dressings	600:620	The present dressings exhibited high swelling, suitable water vapor transmission rate (WVTR), and good mechanical properties and degradability, as well as sustained release of PRP.
33171183	2	54	theme	grafted	543:549	arg1	CMC-PAMAM					570:578	Ag@CMC-PAMAM	567:578	Ag@CMC-PAMAM	567:578	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	2	54	theme	grafted	543:549	arg1	polyamideamine					551:564	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine	445:564	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers	445:597	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	0	55	theme	composite	79:87	arg1	dressing					89:96	carboxymethyl chitosan-polyamideamine alginate composite dressing	32:96	carboxymethyl chitosan-polyamideamine alginate composite dressing for wound treatment	32:116	Development of nanosilver doped carboxymethyl chitosan-polyamideamine alginate composite dressing for wound treatment.
33171183	3	56	theme	PRP	776:778	arg1	properties					714:723	good mechanical properties	698:723	good mechanical properties	698:723	The present dressings exhibited high swelling, suitable water vapor transmission rate (WVTR), and good mechanical properties and degradability, as well as sustained release of PRP.
33171183	3	56	theme	PRP	776:778	arg1	degradability					729:741	degradability	729:741	degradability	729:741	The present dressings exhibited high swelling, suitable water vapor transmission rate (WVTR), and good mechanical properties and degradability, as well as sustained release of PRP.
33171183	3	56	theme	PRP	776:778	arg1	release					765:771	sustained release	755:771	sustained release of PRP	755:778	The present dressings exhibited high swelling, suitable water vapor transmission rate (WVTR), and good mechanical properties and degradability, as well as sustained release of PRP.
33171183	3	56	theme	PRP	776:778	arg1	swelling					637:644	high swelling	632:644	high swelling	632:644	The present dressings exhibited high swelling, suitable water vapor transmission rate (WVTR), and good mechanical properties and degradability, as well as sustained release of PRP.
33171183	3	56	theme	PRP	776:778	arg1	rate					681:684	suitable water vapor transmission rate	647:684	suitable water vapor transmission rate (WVTR)	647:691	The present dressings exhibited high swelling, suitable water vapor transmission rate (WVTR), and good mechanical properties and degradability, as well as sustained release of PRP.
33171183	3	56	theme	PRP	776:778	arg1	WVTR					687:690	WVTR	687:690	WVTR	687:690	The present dressings exhibited high swelling, suitable water vapor transmission rate (WVTR), and good mechanical properties and degradability, as well as sustained release of PRP.
33171183	2	57	theme	cationic	581:588	arg1	polymers					590:597	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers	445:597	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers	445:597	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	3	58	theme	suitable	647:654	arg1	rate					681:684	suitable water vapor transmission rate	647:684	suitable water vapor transmission rate (WVTR)	647:691	The present dressings exhibited high swelling, suitable water vapor transmission rate (WVTR), and good mechanical properties and degradability, as well as sustained release of PRP.
33171183	3	58	theme	suitable	647:654	arg1	WVTR					687:690	WVTR	687:690	WVTR	687:690	The present dressings exhibited high swelling, suitable water vapor transmission rate (WVTR), and good mechanical properties and degradability, as well as sustained release of PRP.
33171183	4	59	theme	@	809:809	arg1	CMC-PAMAM					810:818	Ag@CMC-PAMAM	807:818	Ag@CMC-PAMAM	807:818	Besides, the component of Ag@CMC-PAMAM nanoparticles endow them with excellent antibacterial performance, while the incorporation of PRP promotes the effect of anti-inflammatory and angiogenesis by up-regulating relative activity factor expression of TGF-β1, CD31 and α-SMA and down-regulating the inflammatory-relative genes including TNF-α, IL-6 and IL-1β, all of which promote the closure of wound and produce a superior healing effect to the commercial Aquacel Ag group.
33171183	4	60	theme	wound	1176:1180	arg1	closure					1165:1171	the closure	1161:1171	the closure of wound	1161:1180	Besides, the component of Ag@CMC-PAMAM nanoparticles endow them with excellent antibacterial performance, while the incorporation of PRP promotes the effect of anti-inflammatory and angiogenesis by up-regulating relative activity factor expression of TGF-β1, CD31 and α-SMA and down-regulating the inflammatory-relative genes including TNF-α, IL-6 and IL-1β, all of which promote the closure of wound and produce a superior healing effect to the commercial Aquacel Ag group.
33171183	0	61	theme	alginate	70:77	arg1	dressing					89:96	carboxymethyl chitosan-polyamideamine alginate composite dressing	32:96	carboxymethyl chitosan-polyamideamine alginate composite dressing for wound treatment	32:116	Development of nanosilver doped carboxymethyl chitosan-polyamideamine alginate composite dressing for wound treatment.
33171183	3	62	theme	sustained	755:763	arg1	release					765:771	sustained release	755:771	sustained release of PRP	755:778	The present dressings exhibited high swelling, suitable water vapor transmission rate (WVTR), and good mechanical properties and degradability, as well as sustained release of PRP.
33171183	4	63	theme	PRP	914:916	arg1	incorporation					897:909	the incorporation	893:909	the incorporation of PRP	893:916	Besides, the component of Ag@CMC-PAMAM nanoparticles endow them with excellent antibacterial performance, while the incorporation of PRP promotes the effect of anti-inflammatory and angiogenesis by up-regulating relative activity factor expression of TGF-β1, CD31 and α-SMA and down-regulating the inflammatory-relative genes including TNF-α, IL-6 and IL-1β, all of which promote the closure of wound and produce a superior healing effect to the commercial Aquacel Ag group.
33171183	4	64	theme	Ag	807:808	arg1	CMC-PAMAM					810:818	Ag@CMC-PAMAM	807:818	Ag@CMC-PAMAM	807:818	Besides, the component of Ag@CMC-PAMAM nanoparticles endow them with excellent antibacterial performance, while the incorporation of PRP promotes the effect of anti-inflammatory and angiogenesis by up-regulating relative activity factor expression of TGF-β1, CD31 and α-SMA and down-regulating the inflammatory-relative genes including TNF-α, IL-6 and IL-1β, all of which promote the closure of wound and produce a superior healing effect to the commercial Aquacel Ag group.
33171183	5	65	theme	property	1401:1408	arg1	effect					1377:1382	synergistic effect	1365:1382	synergistic effect of antibacterial property and wound healing	1365:1426	This work indicates that the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing is a promising biomaterial with synergistic effect of antibacterial property and wound healing.
33171183	4	66	theme	CD31	1040:1043	arg1	expression					1018:1027	relative activity factor expression	993:1027	relative activity factor expression of TGF-β1, CD31 and α-SMA	993:1053	Besides, the component of Ag@CMC-PAMAM nanoparticles endow them with excellent antibacterial performance, while the incorporation of PRP promotes the effect of anti-inflammatory and angiogenesis by up-regulating relative activity factor expression of TGF-β1, CD31 and α-SMA and down-regulating the inflammatory-relative genes including TNF-α, IL-6 and IL-1β, all of which promote the closure of wound and produce a superior healing effect to the commercial Aquacel Ag group.
33171183	3	67	theme	high	632:635	arg1	swelling					637:644	high swelling	632:644	high swelling	632:644	The present dressings exhibited high swelling, suitable water vapor transmission rate (WVTR), and good mechanical properties and degradability, as well as sustained release of PRP.
33171183	1	68	theme	infected	146:153	arg1	wounds					155:160	the infected wounds	142:160	the infected wounds caused by bacterial even multi-resistant bacterial strains	142:219	Nowadays, treatment to the infected wounds caused by bacterial even multi-resistant bacterial strains and subsequently complete skin regeneration remain a critical clinical challenge.
33171183	4	69	theme	CMC-PAMAM	810:818	arg1	component					794:802	the component	790:802	the component of Ag@CMC-PAMAM	790:818	Besides, the component of Ag@CMC-PAMAM nanoparticles endow them with excellent antibacterial performance, while the incorporation of PRP promotes the effect of anti-inflammatory and angiogenesis by up-regulating relative activity factor expression of TGF-β1, CD31 and α-SMA and down-regulating the inflammatory-relative genes including TNF-α, IL-6 and IL-1β, all of which promote the closure of wound and produce a superior healing effect to the commercial Aquacel Ag group.
33171183	2	70	theme	@	569:569	arg1	CMC-PAMAM					570:578	Ag@CMC-PAMAM	567:578	Ag@CMC-PAMAM	567:578	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	2	70	theme	@	569:569	arg1	polyamideamine					551:564	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine	445:564	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers	445:597	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	3	71	theme	transmission	668:679	arg1	rate					681:684	suitable water vapor transmission rate	647:684	suitable water vapor transmission rate (WVTR)	647:691	The present dressings exhibited high swelling, suitable water vapor transmission rate (WVTR), and good mechanical properties and degradability, as well as sustained release of PRP.
33171183	3	71	theme	transmission	668:679	arg1	WVTR					687:690	WVTR	687:690	WVTR	687:690	The present dressings exhibited high swelling, suitable water vapor transmission rate (WVTR), and good mechanical properties and degradability, as well as sustained release of PRP.
33171183	5	72	theme	@	1303:1303	arg1	biomaterial					1348:1358	a promising biomaterial	1336:1358	a promising biomaterial with synergistic effect of antibacterial property and wound healing	1336:1426	This work indicates that the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing is a promising biomaterial with synergistic effect of antibacterial property and wound healing.
33171183	5	72	theme	@	1303:1303	arg1	dressing					1324:1331	the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing	1281:1331	the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing	1281:1331	This work indicates that the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing is a promising biomaterial with synergistic effect of antibacterial property and wound healing.
33171183	2	73	theme	Ag	510:511	arg1	chitosan					534:541	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan	445:541	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers	445:597	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	4	74	theme	inflammatory-relative	1079:1099	arg1	genes					1101:1105	the inflammatory-relative genes	1075:1105	the inflammatory-relative genes	1075:1105	Besides, the component of Ag@CMC-PAMAM nanoparticles endow them with excellent antibacterial performance, while the incorporation of PRP promotes the effect of anti-inflammatory and angiogenesis by up-regulating relative activity factor expression of TGF-β1, CD31 and α-SMA and down-regulating the inflammatory-relative genes including TNF-α, IL-6 and IL-1β, all of which promote the closure of wound and produce a superior healing effect to the commercial Aquacel Ag group.
33171183	4	74	theme	inflammatory-relative	1079:1099	arg1	IL-1β					1133:1137	IL-1β	1133:1137	IL-1β	1133:1137	Besides, the component of Ag@CMC-PAMAM nanoparticles endow them with excellent antibacterial performance, while the incorporation of PRP promotes the effect of anti-inflammatory and angiogenesis by up-regulating relative activity factor expression of TGF-β1, CD31 and α-SMA and down-regulating the inflammatory-relative genes including TNF-α, IL-6 and IL-1β, all of which promote the closure of wound and produce a superior healing effect to the commercial Aquacel Ag group.
33171183	4	74	theme	inflammatory-relative	1079:1099	arg1	IL-6					1124:1127	IL-6	1124:1127	IL-6	1124:1127	Besides, the component of Ag@CMC-PAMAM nanoparticles endow them with excellent antibacterial performance, while the incorporation of PRP promotes the effect of anti-inflammatory and angiogenesis by up-regulating relative activity factor expression of TGF-β1, CD31 and α-SMA and down-regulating the inflammatory-relative genes including TNF-α, IL-6 and IL-1β, all of which promote the closure of wound and produce a superior healing effect to the commercial Aquacel Ag group.
33171183	4	74	theme	inflammatory-relative	1079:1099	arg1	TNF-α					1117:1121	TNF-α	1117:1121	TNF-α	1117:1121	Besides, the component of Ag@CMC-PAMAM nanoparticles endow them with excellent antibacterial performance, while the incorporation of PRP promotes the effect of anti-inflammatory and angiogenesis by up-regulating relative activity factor expression of TGF-β1, CD31 and α-SMA and down-regulating the inflammatory-relative genes including TNF-α, IL-6 and IL-1β, all of which promote the closure of wound and produce a superior healing effect to the commercial Aquacel Ag group.
33171183	1	75	theme	critical	274:281	arg1	challenge					292:300	a critical clinical challenge	272:300	a critical clinical challenge	272:300	Nowadays, treatment to the infected wounds caused by bacterial even multi-resistant bacterial strains and subsequently complete skin regeneration remain a critical clinical challenge.
33171183	2	76	theme	based	471:475	arg1	chitosan					534:541	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan	445:541	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers	445:597	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	4	77	theme	relative	993:1000	arg1	expression					1018:1027	relative activity factor expression	993:1027	relative activity factor expression of TGF-β1, CD31 and α-SMA	993:1053	Besides, the component of Ag@CMC-PAMAM nanoparticles endow them with excellent antibacterial performance, while the incorporation of PRP promotes the effect of anti-inflammatory and angiogenesis by up-regulating relative activity factor expression of TGF-β1, CD31 and α-SMA and down-regulating the inflammatory-relative genes including TNF-α, IL-6 and IL-1β, all of which promote the closure of wound and produce a superior healing effect to the commercial Aquacel Ag group.
33171183	2	78	theme	sodium	449:454	arg1	chitosan					534:541	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan	445:541	the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers	445:597	Herein, a novel multi-functional platform (Alg/1.0Ag@CMC-PAMAM/PRP) was prepared as wound dressings by mixing platelet rich plasma (PRP) with the sodium alginate (Alg) based dressing containing nano silver (Ag)-doped carboxymethyl chitosan grafted polyamideamine (Ag@CMC-PAMAM) cationic polymers.
33171183	5	79	theme	synergistic	1365:1375	arg1	effect					1377:1382	synergistic effect	1365:1382	synergistic effect of antibacterial property and wound healing	1365:1426	This work indicates that the prepared Alg/1.0Ag@CMC-PAMAM/PRP wound dressing is a promising biomaterial with synergistic effect of antibacterial property and wound healing.
33171183	1	80	theme	clinical	283:290	arg1	challenge					292:300	a critical clinical challenge	272:300	a critical clinical challenge	272:300	Nowadays, treatment to the infected wounds caused by bacterial even multi-resistant bacterial strains and subsequently complete skin regeneration remain a critical clinical challenge.
32812228	7	0	theme	treatments	1340:1349	arg1	acid					1288:1291	free fatty acid	1277:1291	free fatty acid (FFA)	1277:1297	The result indicated that pH, peroxide, free fatty acid (FFA), and thiobarbituric acide (TBA) value of treatments were significantly (P ˂ 0.05) lower than controls however significantly (P < 0.05) higher DPPH activity was observed in all treatments.
32812228	7	0	theme	treatments	1340:1349	arg1	value					1331:1335	thiobarbituric acide (TBA) value	1304:1335	thiobarbituric acide (TBA) value of treatments	1304:1349	The result indicated that pH, peroxide, free fatty acid (FFA), and thiobarbituric acide (TBA) value of treatments were significantly (P ˂ 0.05) lower than controls however significantly (P < 0.05) higher DPPH activity was observed in all treatments.
32812228	7	0	theme	treatments	1340:1349	arg1	peroxide					1267:1274	peroxide	1267:1274	peroxide	1267:1274	The result indicated that pH, peroxide, free fatty acid (FFA), and thiobarbituric acide (TBA) value of treatments were significantly (P ˂ 0.05) lower than controls however significantly (P < 0.05) higher DPPH activity was observed in all treatments.
32812228	7	0	theme	treatments	1340:1349	arg1	FFA					1294:1296	FFA	1294:1296	FFA	1294:1296	The result indicated that pH, peroxide, free fatty acid (FFA), and thiobarbituric acide (TBA) value of treatments were significantly (P ˂ 0.05) lower than controls however significantly (P < 0.05) higher DPPH activity was observed in all treatments.
32812228	7	0	theme	treatments	1340:1349	arg1	pH					1263:1264	pH	1263:1264	pH	1263:1264	The result indicated that pH, peroxide, free fatty acid (FFA), and thiobarbituric acide (TBA) value of treatments were significantly (P ˂ 0.05) lower than controls however significantly (P < 0.05) higher DPPH activity was observed in all treatments.
32812228	13	1	theme	packaging	2271:2279	arg1	conditions					2281:2290	product-specific packaging conditions	2254:2290	product-specific packaging conditions	2254:2290	The composite-active bio-based films have a huge potential to be molded for specific film properties based on requirements of product-specific packaging conditions.
32812228	1	2	theme	developed	423:431	arg1	film					433:436	the developed film	419:436	the developed film at refrigeration storage	419:461	The present study was aimed to develop Manihot esculenta and Carrageenan bio-based composite active film functionalized with anise, caraway, and nutmeg essential oils (EOs) and to assess the shelf life of chicken nuggets wrapped with the developed film at refrigeration storage.
32812228	1	3	theme	active	278:283	arg1	film					285:288	Carrageenan bio-based composite active film	246:288	Carrageenan bio-based composite active film	246:288	The present study was aimed to develop Manihot esculenta and Carrageenan bio-based composite active film functionalized with anise, caraway, and nutmeg essential oils (EOs) and to assess the shelf life of chicken nuggets wrapped with the developed film at refrigeration storage.
32812228	4	4	theme	significant	798:808	arg1	changes					821:827	No significant (P ˃ 0.05) changes	795:827	No significant (P ˃ 0.05) changes in thickness and Water Vapor Transmission Rate (WVTR)	795:881	No significant (P ˃ 0.05) changes in thickness and Water Vapor Transmission Rate (WVTR) were observed.
32812228	8	5	dep	significantly	1566:1578	arg1	P					1581:1581	P ˂ 0.01	1581:1588	P ˂ 0.01	1581:1588	The total plate count, psychrophilic count and, yeast and mold count were also significantly (P ˂ 0.01) lower in treatment groups and were within the permissible limits.
32812228	7	6	theme	TBA	1326:1328	arg1	value					1331:1335	thiobarbituric acide (TBA) value	1304:1335	thiobarbituric acide (TBA) value of treatments	1304:1349	The result indicated that pH, peroxide, free fatty acid (FFA), and thiobarbituric acide (TBA) value of treatments were significantly (P ˂ 0.05) lower than controls however significantly (P < 0.05) higher DPPH activity was observed in all treatments.
32812228	11	7	theme	meat	1945:1948	arg1	packaging					1932:1940	packaging	1932:1940	packaging of meat and meat products	1932:1966	PRACTICAL APPLICATION: The majority of films used for packaging of meat and meat products are derived from synthetic "plastic" materials.
32812228	9	8	theme	storage	1711:1717	arg1	period					1719:1724	whole storage period	1705:1724	whole storage period of 15 days	1705:1735	The treated samples were well acceptable during whole storage period of 15 days.
32812228	3	9	theme	significant	658:668	arg1	decrease					681:688	significant (P ˂ 0.05) decrease	658:688	significant (P ˂ 0.05) decrease in tensile strength	658:708	Incorporation of EOs in the film resulted in significant (P ˂ 0.05) decrease in tensile strength and water activity while elongation at break was significantly (P ˂ 0.05) increased.
32812228	6	10	theme	refrigeration	1131:1143	arg1	4 ± 1 °C					1158:1165	4 ± 1 °C	1158:1165	4 ± 1 °C	1158:1165	Chicken nuggets overwrapped with aforementioned films were stored aerobically at refrigeration temperature (4 ± 1 °C) to evaluate antimicrobial, antioxidant, and sensory characteristics.
32812228	6	10	theme	refrigeration	1131:1143	arg1	temperature					1145:1155	refrigeration temperature	1131:1155	refrigeration temperature (4 ± 1 °C)	1131:1166	Chicken nuggets overwrapped with aforementioned films were stored aerobically at refrigeration temperature (4 ± 1 °C) to evaluate antimicrobial, antioxidant, and sensory characteristics.
32812228	1	11	theme	present	189:195	arg1	study					197:201	The present study	185:201	The present study	185:201	The present study was aimed to develop Manihot esculenta and Carrageenan bio-based composite active film functionalized with anise, caraway, and nutmeg essential oils (EOs) and to assess the shelf life of chicken nuggets wrapped with the developed film at refrigeration storage.
32812228	7	12	theme	acide	1319:1323	arg1	value					1331:1335	thiobarbituric acide (TBA) value	1304:1335	thiobarbituric acide (TBA) value of treatments	1304:1349	The result indicated that pH, peroxide, free fatty acid (FFA), and thiobarbituric acide (TBA) value of treatments were significantly (P ˂ 0.05) lower than controls however significantly (P < 0.05) higher DPPH activity was observed in all treatments.
32812228	2	13	theme	nutmeg	577:582	arg1	EO					584:585	nutmeg EO	577:585	nutmeg EO	577:585	Overall, the Minimum Inhibitory Concentration (MIC) values of the three EOs ranged from 0.4 to 0.8% v/v of which nutmeg EO was found most effective.
32812228	11	14	attach	derived	1972:1978	arg2	majority					1905:1912	The majority	1901:1912	The majority of films used for packaging of meat and meat products	1901:1966	PRACTICAL APPLICATION: The majority of films used for packaging of meat and meat products are derived from synthetic "plastic" materials.
32812228	11	14	attach	derived	1972:1978	arg1	materials					2005:2013	synthetic "plastic" materials	1985:2013	synthetic "plastic" materials	1985:2013	PRACTICAL APPLICATION: The majority of films used for packaging of meat and meat products are derived from synthetic "plastic" materials.
32812228	13	15	theme	conditions	2281:2290	arg1	requirements					2238:2249	requirements	2238:2249	requirements of product-specific packaging conditions	2238:2290	The composite-active bio-based films have a huge potential to be molded for specific film properties based on requirements of product-specific packaging conditions.
32812228	2	16	theme	EOs	536:538	arg1	values					516:521	the Minimum Inhibitory Concentration (MIC) values	473:521	the Minimum Inhibitory Concentration (MIC) values of the three EOs	473:538	Overall, the Minimum Inhibitory Concentration (MIC) values of the three EOs ranged from 0.4 to 0.8% v/v of which nutmeg EO was found most effective.
32812228	7	17	theme	free	1277:1280	arg1	acid					1288:1291	free fatty acid	1277:1291	free fatty acid (FFA)	1277:1297	The result indicated that pH, peroxide, free fatty acid (FFA), and thiobarbituric acide (TBA) value of treatments were significantly (P ˂ 0.05) lower than controls however significantly (P < 0.05) higher DPPH activity was observed in all treatments.
32812228	7	17	theme	free	1277:1280	arg1	FFA					1294:1296	FFA	1294:1296	FFA	1294:1296	The result indicated that pH, peroxide, free fatty acid (FFA), and thiobarbituric acide (TBA) value of treatments were significantly (P ˂ 0.05) lower than controls however significantly (P < 0.05) higher DPPH activity was observed in all treatments.
32812228	9	18	theme	days	1732:1735	arg1	period					1719:1724	whole storage period	1705:1724	whole storage period of 15 days	1705:1735	The treated samples were well acceptable during whole storage period of 15 days.
32812228	1	19	theme	shelf	376:380	arg1	life					382:385	the shelf life	372:385	the shelf life of chicken nuggets wrapped with the developed film at refrigeration storage	372:461	The present study was aimed to develop Manihot esculenta and Carrageenan bio-based composite active film functionalized with anise, caraway, and nutmeg essential oils (EOs) and to assess the shelf life of chicken nuggets wrapped with the developed film at refrigeration storage.
32812228	2	20	dep	%	562:562	arg1	0.8					559:561	0.8	559:561	0.8	559:561	Overall, the Minimum Inhibitory Concentration (MIC) values of the three EOs ranged from 0.4 to 0.8% v/v of which nutmeg EO was found most effective.
32812228	10	21	theme	film	1792:1795	arg1	application					1742:1752	The application	1738:1752	The application of composite, active edible bio-based film	1738:1795	The application of composite, active edible bio-based film was found proficient in confining product quality attributes throughout storage.
32812228	0	22	theme	chicken	144:150	arg1	nuggets					152:158	chicken nuggets	144:158	chicken nuggets	144:158	Materialization of novel composite bio-based active edible film functionalized with essential oils on antimicrobial and antioxidative aspect of chicken nuggets during extended storage.
32812228	4	23	from	changes	821:827	arg1	WVTR					877:880	WVTR	877:880	WVTR	877:880	No significant (P ˃ 0.05) changes in thickness and Water Vapor Transmission Rate (WVTR) were observed.
32812228	4	23	from	changes	821:827	arg1	Rate					871:874	Water Vapor Transmission Rate	846:874	Water Vapor Transmission Rate (WVTR)	846:881	No significant (P ˃ 0.05) changes in thickness and Water Vapor Transmission Rate (WVTR) were observed.
32812228	4	23	from	changes	821:827	arg1	thickness					832:840	thickness	832:840	thickness	832:840	No significant (P ˃ 0.05) changes in thickness and Water Vapor Transmission Rate (WVTR) were observed.
32812228	1	24	theme	Manihot	224:230	arg1	esculenta					232:240	Manihot esculenta	224:240	Manihot esculenta	224:240	The present study was aimed to develop Manihot esculenta and Carrageenan bio-based composite active film functionalized with anise, caraway, and nutmeg essential oils (EOs) and to assess the shelf life of chicken nuggets wrapped with the developed film at refrigeration storage.
32812228	3	25	theme	tensile	693:699	arg1	strength					701:708	tensile strength	693:708	tensile strength	693:708	Incorporation of EOs in the film resulted in significant (P ˂ 0.05) decrease in tensile strength and water activity while elongation at break was significantly (P ˂ 0.05) increased.
32812228	6	26	theme	antioxidant	1195:1205	arg1	characteristics					1220:1234	antimicrobial, antioxidant, and sensory characteristics	1180:1234	antimicrobial, antioxidant, and sensory characteristics	1180:1234	Chicken nuggets overwrapped with aforementioned films were stored aerobically at refrigeration temperature (4 ± 1 °C) to evaluate antimicrobial, antioxidant, and sensory characteristics.
32812228	1	27	theme	nuggets	398:404	arg1	life					382:385	the shelf life	372:385	the shelf life of chicken nuggets wrapped with the developed film at refrigeration storage	372:461	The present study was aimed to develop Manihot esculenta and Carrageenan bio-based composite active film functionalized with anise, caraway, and nutmeg essential oils (EOs) and to assess the shelf life of chicken nuggets wrapped with the developed film at refrigeration storage.
32812228	8	28	theme	˂	1583:1583	arg1	P					1581:1581	P ˂ 0.01	1581:1588	P ˂ 0.01	1581:1588	The total plate count, psychrophilic count and, yeast and mold count were also significantly (P ˂ 0.01) lower in treatment groups and were within the permissible limits.
32812228	5	29	theme	nutmeg	1012:1017	arg1	concentration					988:1000	0.5, 1, and 1% concentration	973:1000	0.5, 1, and 1% concentration of anise, nutmeg, and caraway EO	973:1033	Based on physicomechanical and sensory evaluation, films incorporated with 0.5, 1, and 1% concentration of anise, nutmeg, and caraway EO were selected.
32812228	7	30	theme	DPPH	1441:1444	arg1	activity					1446:1453	significantly (P < 0.05) higher DPPH activity	1409:1453	significantly (P < 0.05) higher DPPH activity	1409:1453	The result indicated that pH, peroxide, free fatty acid (FFA), and thiobarbituric acide (TBA) value of treatments were significantly (P ˂ 0.05) lower than controls however significantly (P < 0.05) higher DPPH activity was observed in all treatments.
32812228	6	31	theme	antimicrobial	1180:1192	arg1	characteristics					1220:1234	antimicrobial, antioxidant, and sensory characteristics	1180:1234	antimicrobial, antioxidant, and sensory characteristics	1180:1234	Chicken nuggets overwrapped with aforementioned films were stored aerobically at refrigeration temperature (4 ± 1 °C) to evaluate antimicrobial, antioxidant, and sensory characteristics.
32812228	3	32	dep	significantly	759:771	arg1	P					774:774	P ˂ 0.05	774:781	P ˂ 0.05	774:781	Incorporation of EOs in the film resulted in significant (P ˂ 0.05) decrease in tensile strength and water activity while elongation at break was significantly (P ˂ 0.05) increased.
32812228	2	33	theme	Concentration	496:508	arg1	values					516:521	the Minimum Inhibitory Concentration (MIC) values	473:521	the Minimum Inhibitory Concentration (MIC) values of the three EOs	473:538	Overall, the Minimum Inhibitory Concentration (MIC) values of the three EOs ranged from 0.4 to 0.8% v/v of which nutmeg EO was found most effective.
32812228	2	34	theme	Minimum	477:483	arg1	MIC					511:513	MIC	511:513	MIC	511:513	Overall, the Minimum Inhibitory Concentration (MIC) values of the three EOs ranged from 0.4 to 0.8% v/v of which nutmeg EO was found most effective.
32812228	2	34	theme	Minimum	477:483	arg1	Concentration					496:508	Minimum Inhibitory Concentration	477:508	the Minimum Inhibitory Concentration (MIC) values of the three EOs	473:538	Overall, the Minimum Inhibitory Concentration (MIC) values of the three EOs ranged from 0.4 to 0.8% v/v of which nutmeg EO was found most effective.
32812228	0	35	theme	essential	84:92	arg1	oils					94:97	essential oils	84:97	essential oils on antimicrobial and antioxidative aspect of chicken nuggets during extended storage	84:182	Materialization of novel composite bio-based active edible film functionalized with essential oils on antimicrobial and antioxidative aspect of chicken nuggets during extended storage.
32812228	5	36	theme	anise	1005:1009	arg1	concentration					988:1000	0.5, 1, and 1% concentration	973:1000	0.5, 1, and 1% concentration of anise, nutmeg, and caraway EO	973:1033	Based on physicomechanical and sensory evaluation, films incorporated with 0.5, 1, and 1% concentration of anise, nutmeg, and caraway EO were selected.
32812228	4	37	theme	Water	846:850	arg1	WVTR					877:880	WVTR	877:880	WVTR	877:880	No significant (P ˃ 0.05) changes in thickness and Water Vapor Transmission Rate (WVTR) were observed.
32812228	4	37	theme	Water	846:850	arg1	Rate					871:874	Water Vapor Transmission Rate	846:874	Water Vapor Transmission Rate (WVTR)	846:881	No significant (P ˃ 0.05) changes in thickness and Water Vapor Transmission Rate (WVTR) were observed.
32812228	10	38	theme	product	1831:1837	arg1	attributes					1847:1856	product quality attributes	1831:1856	product quality attributes	1831:1856	The application of composite, active edible bio-based film was found proficient in confining product quality attributes throughout storage.
32812228	4	39	theme	Transmission	858:869	arg1	WVTR					877:880	WVTR	877:880	WVTR	877:880	No significant (P ˃ 0.05) changes in thickness and Water Vapor Transmission Rate (WVTR) were observed.
32812228	4	39	theme	Transmission	858:869	arg1	Rate					871:874	Water Vapor Transmission Rate	846:874	Water Vapor Transmission Rate (WVTR)	846:881	No significant (P ˃ 0.05) changes in thickness and Water Vapor Transmission Rate (WVTR) were observed.
32812228	0	40	theme	active	45:50	arg1	film					59:62	novel composite bio-based active edible film	19:62	novel composite bio-based active edible film	19:62	Materialization of novel composite bio-based active edible film functionalized with essential oils on antimicrobial and antioxidative aspect of chicken nuggets during extended storage.
32812228	13	41	theme	product-specific	2254:2269	arg1	conditions					2281:2290	product-specific packaging conditions	2254:2290	product-specific packaging conditions	2254:2290	The composite-active bio-based films have a huge potential to be molded for specific film properties based on requirements of product-specific packaging conditions.
32812228	10	42	dep	composite	1757:1765	arg1	active					1768:1773	active	1768:1773	active	1768:1773	The application of composite, active edible bio-based film was found proficient in confining product quality attributes throughout storage.
32812228	3	43	from	decrease	681:688	arg1	strength					701:708	tensile strength	693:708	tensile strength	693:708	Incorporation of EOs in the film resulted in significant (P ˂ 0.05) decrease in tensile strength and water activity while elongation at break was significantly (P ˂ 0.05) increased.
32812228	5	44	theme	EO	1032:1033	arg1	concentration					988:1000	0.5, 1, and 1% concentration	973:1000	0.5, 1, and 1% concentration of anise, nutmeg, and caraway EO	973:1033	Based on physicomechanical and sensory evaluation, films incorporated with 0.5, 1, and 1% concentration of anise, nutmeg, and caraway EO were selected.
32812228	13	45	theme	film	2213:2216	arg1	properties					2218:2227	specific film properties	2204:2227	specific film properties based on requirements of product-specific packaging conditions	2204:2290	The composite-active bio-based films have a huge potential to be molded for specific film properties based on requirements of product-specific packaging conditions.
32812228	11	46	theme	meat	1954:1957	arg1	packaging					1932:1940	packaging	1932:1940	packaging of meat and meat products	1932:1966	PRACTICAL APPLICATION: The majority of films used for packaging of meat and meat products are derived from synthetic "plastic" materials.
32812228	3	47	from	break	749:753	arg1	elongation					735:744	elongation	735:744	elongation at break	735:753	Incorporation of EOs in the film resulted in significant (P ˂ 0.05) decrease in tensile strength and water activity while elongation at break was significantly (P ˂ 0.05) increased.
32812228	0	48	theme	novel	19:23	arg1	film					59:62	novel composite bio-based active edible film	19:62	novel composite bio-based active edible film	19:62	Materialization of novel composite bio-based active edible film functionalized with essential oils on antimicrobial and antioxidative aspect of chicken nuggets during extended storage.
32812228	7	49	located	observed	1459:1466	arg2	activity					1446:1453	significantly (P < 0.05) higher DPPH activity	1409:1453	significantly (P < 0.05) higher DPPH activity	1409:1453	The result indicated that pH, peroxide, free fatty acid (FFA), and thiobarbituric acide (TBA) value of treatments were significantly (P ˂ 0.05) lower than controls however significantly (P < 0.05) higher DPPH activity was observed in all treatments.
32812228	7	49	located	observed	1459:1466	arg1	treatments					1475:1484	all treatments	1471:1484	all treatments	1471:1484	The result indicated that pH, peroxide, free fatty acid (FFA), and thiobarbituric acide (TBA) value of treatments were significantly (P ˂ 0.05) lower than controls however significantly (P < 0.05) higher DPPH activity was observed in all treatments.
32812228	0	50	theme	bio-based	35:43	arg1	film					59:62	novel composite bio-based active edible film	19:62	novel composite bio-based active edible film	19:62	Materialization of novel composite bio-based active edible film functionalized with essential oils on antimicrobial and antioxidative aspect of chicken nuggets during extended storage.
32812228	3	51	dep	significant	658:668	arg1	P					671:671	P ˂ 0.05	671:678	P ˂ 0.05	671:678	Incorporation of EOs in the film resulted in significant (P ˂ 0.05) decrease in tensile strength and water activity while elongation at break was significantly (P ˂ 0.05) increased.
32812228	5	52	theme	physicomechanical	907:923	arg1	evaluation					937:946	physicomechanical and sensory evaluation	907:946	physicomechanical and sensory evaluation	907:946	Based on physicomechanical and sensory evaluation, films incorporated with 0.5, 1, and 1% concentration of anise, nutmeg, and caraway EO were selected.
32812228	5	53	dep	%	986:986	arg1	1					985:985	1	985:985	1	985:985	Based on physicomechanical and sensory evaluation, films incorporated with 0.5, 1, and 1% concentration of anise, nutmeg, and caraway EO were selected.
32812228	5	53	dep	%	986:986	arg1	1					978:978	1	978:978	1	978:978	Based on physicomechanical and sensory evaluation, films incorporated with 0.5, 1, and 1% concentration of anise, nutmeg, and caraway EO were selected.
32812228	3	54	from	activity	720:727	arg1	strength					701:708	tensile strength	693:708	tensile strength	693:708	Incorporation of EOs in the film resulted in significant (P ˂ 0.05) decrease in tensile strength and water activity while elongation at break was significantly (P ˂ 0.05) increased.
32812228	0	55	theme	edible	52:57	arg1	film					59:62	novel composite bio-based active edible film	19:62	novel composite bio-based active edible film	19:62	Materialization of novel composite bio-based active edible film functionalized with essential oils on antimicrobial and antioxidative aspect of chicken nuggets during extended storage.
32812228	1	56	theme	nutmeg	330:335	arg1	EOs					353:355	EOs	353:355	EOs	353:355	The present study was aimed to develop Manihot esculenta and Carrageenan bio-based composite active film functionalized with anise, caraway, and nutmeg essential oils (EOs) and to assess the shelf life of chicken nuggets wrapped with the developed film at refrigeration storage.
32812228	1	56	theme	nutmeg	330:335	arg1	oils					347:350	nutmeg essential oils	330:350	nutmeg essential oils (EOs)	330:356	The present study was aimed to develop Manihot esculenta and Carrageenan bio-based composite active film functionalized with anise, caraway, and nutmeg essential oils (EOs) and to assess the shelf life of chicken nuggets wrapped with the developed film at refrigeration storage.
32812228	5	57	theme	sensory	929:935	arg1	evaluation					937:946	physicomechanical and sensory evaluation	907:946	physicomechanical and sensory evaluation	907:946	Based on physicomechanical and sensory evaluation, films incorporated with 0.5, 1, and 1% concentration of anise, nutmeg, and caraway EO were selected.
32812228	10	58	theme	bio-based	1782:1790	arg1	film					1792:1795	composite, active edible bio-based film	1757:1795	composite, active edible bio-based film	1757:1795	The application of composite, active edible bio-based film was found proficient in confining product quality attributes throughout storage.
32812228	9	59	theme	treated	1661:1667	arg1	samples					1669:1675	The treated samples	1657:1675	The treated samples	1657:1675	The treated samples were well acceptable during whole storage period of 15 days.
32812228	9	59	theme	treated	1661:1667	arg1	acceptable					1687:1696	acceptable	1687:1696	acceptable	1687:1696	The treated samples were well acceptable during whole storage period of 15 days.
32812228	3	60	from	Incorporation	613:625	arg1	film					641:644	the film	637:644	the film	637:644	Incorporation of EOs in the film resulted in significant (P ˂ 0.05) decrease in tensile strength and water activity while elongation at break was significantly (P ˂ 0.05) increased.
32812228	8	61	theme	total	1491:1495	arg1	lower					1591:1595	lower	1591:1595	lower	1591:1595	The total plate count, psychrophilic count and, yeast and mold count were also significantly (P ˂ 0.01) lower in treatment groups and were within the permissible limits.
32812228	8	61	theme	total	1491:1495	arg1	count					1503:1507	The total plate count	1487:1507	The total plate count	1487:1507	The total plate count, psychrophilic count and, yeast and mold count were also significantly (P ˂ 0.01) lower in treatment groups and were within the permissible limits.
32812228	1	62	theme	Carrageenan	246:256	arg1	film					285:288	Carrageenan bio-based composite active film	246:288	Carrageenan bio-based composite active film	246:288	The present study was aimed to develop Manihot esculenta and Carrageenan bio-based composite active film functionalized with anise, caraway, and nutmeg essential oils (EOs) and to assess the shelf life of chicken nuggets wrapped with the developed film at refrigeration storage.
32812228	11	63	theme	"	2003:2003	arg1	materials					2005:2013	synthetic "plastic" materials	1985:2013	synthetic "plastic" materials	1985:2013	PRACTICAL APPLICATION: The majority of films used for packaging of meat and meat products are derived from synthetic "plastic" materials.
32812228	1	64	theme	composite	268:276	arg1	film					285:288	Carrageenan bio-based composite active film	246:288	Carrageenan bio-based composite active film	246:288	The present study was aimed to develop Manihot esculenta and Carrageenan bio-based composite active film functionalized with anise, caraway, and nutmeg essential oils (EOs) and to assess the shelf life of chicken nuggets wrapped with the developed film at refrigeration storage.
32812228	11	65	dep	APPLICATION	1888:1898	arg1	derived					1972:1978	derived	1972:1978	are derived from synthetic "plastic" materials	1968:2013	PRACTICAL APPLICATION: The majority of films used for packaging of meat and meat products are derived from synthetic "plastic" materials.
32812228	8	66	theme	psychrophilic	1510:1522	arg1	count					1524:1528	psychrophilic count	1510:1528	psychrophilic count	1510:1528	The total plate count, psychrophilic count and, yeast and mold count were also significantly (P ˂ 0.01) lower in treatment groups and were within the permissible limits.
32812228	11	67	theme	films	1917:1921	arg1	majority					1905:1912	The majority	1901:1912	The majority of films used for packaging of meat and meat products	1901:1966	PRACTICAL APPLICATION: The majority of films used for packaging of meat and meat products are derived from synthetic "plastic" materials.
32812228	9	68	theme	whole	1705:1709	arg1	period					1719:1724	whole storage period	1705:1724	whole storage period of 15 days	1705:1735	The treated samples were well acceptable during whole storage period of 15 days.
32812228	3	69	theme	˂	673:673	arg1	P					671:671	P ˂ 0.05	671:678	P ˂ 0.05	671:678	Incorporation of EOs in the film resulted in significant (P ˂ 0.05) decrease in tensile strength and water activity while elongation at break was significantly (P ˂ 0.05) increased.
32812228	1	70	theme	refrigeration	441:453	arg1	storage					455:461	refrigeration storage	441:461	refrigeration storage	441:461	The present study was aimed to develop Manihot esculenta and Carrageenan bio-based composite active film functionalized with anise, caraway, and nutmeg essential oils (EOs) and to assess the shelf life of chicken nuggets wrapped with the developed film at refrigeration storage.
32812228	13	71	contain	have	2165:2168	arg2	potential					2177:2185	a huge potential	2170:2185	a huge potential to be molded for specific film properties based on requirements of product-specific packaging conditions	2170:2290	The composite-active bio-based films have a huge potential to be molded for specific film properties based on requirements of product-specific packaging conditions.
32812228	13	71	contain	have	2165:2168	arg1	films					2159:2163	The composite-active bio-based films	2128:2163	The composite-active bio-based films	2128:2163	The composite-active bio-based films have a huge potential to be molded for specific film properties based on requirements of product-specific packaging conditions.
32812228	7	72	theme	thiobarbituric	1304:1317	arg1	value					1331:1335	thiobarbituric acide (TBA) value	1304:1335	thiobarbituric acide (TBA) value of treatments	1304:1349	The result indicated that pH, peroxide, free fatty acid (FFA), and thiobarbituric acide (TBA) value of treatments were significantly (P ˂ 0.05) lower than controls however significantly (P < 0.05) higher DPPH activity was observed in all treatments.
32812228	13	73	theme	bio-based	2149:2157	arg1	films					2159:2163	The composite-active bio-based films	2128:2163	The composite-active bio-based films	2128:2163	The composite-active bio-based films have a huge potential to be molded for specific film properties based on requirements of product-specific packaging conditions.
32812228	10	74	theme	composite	1757:1765	arg1	film					1792:1795	composite, active edible bio-based film	1757:1795	composite, active edible bio-based film	1757:1795	The application of composite, active edible bio-based film was found proficient in confining product quality attributes throughout storage.
32812228	8	75	theme	mold	1545:1548	arg1	count					1550:1554	mold count	1545:1554	mold count	1545:1554	The total plate count, psychrophilic count and, yeast and mold count were also significantly (P ˂ 0.01) lower in treatment groups and were within the permissible limits.
32812228	11	76	theme	PRACTICAL	1878:1886	arg1	APPLICATION					1888:1898	PRACTICAL APPLICATION	1878:1898	PRACTICAL APPLICATION: The majority of films used for packaging of meat and meat products are derived from synthetic "plastic" materials.	1878:2014	PRACTICAL APPLICATION: The majority of films used for packaging of meat and meat products are derived from synthetic "plastic" materials.
32812228	0	77	from	oils	94:97	arg1	aspect					134:139	antimicrobial and antioxidative aspect	102:139	aspect	134:139	Materialization of novel composite bio-based active edible film functionalized with essential oils on antimicrobial and antioxidative aspect of chicken nuggets during extended storage.
32812228	7	78	theme	fatty	1282:1286	arg1	acid					1288:1291	free fatty acid	1277:1291	free fatty acid (FFA)	1277:1297	The result indicated that pH, peroxide, free fatty acid (FFA), and thiobarbituric acide (TBA) value of treatments were significantly (P ˂ 0.05) lower than controls however significantly (P < 0.05) higher DPPH activity was observed in all treatments.
32812228	7	78	theme	fatty	1282:1286	arg1	FFA					1294:1296	FFA	1294:1296	FFA	1294:1296	The result indicated that pH, peroxide, free fatty acid (FFA), and thiobarbituric acide (TBA) value of treatments were significantly (P ˂ 0.05) lower than controls however significantly (P < 0.05) higher DPPH activity was observed in all treatments.
32812228	6	79	theme	sensory	1212:1218	arg1	characteristics					1220:1234	antimicrobial, antioxidant, and sensory characteristics	1180:1234	antimicrobial, antioxidant, and sensory characteristics	1180:1234	Chicken nuggets overwrapped with aforementioned films were stored aerobically at refrigeration temperature (4 ± 1 °C) to evaluate antimicrobial, antioxidant, and sensory characteristics.
32812228	2	80	dep	0.8	559:561	arg1	to					556:557	to	556:557	to	556:557	Overall, the Minimum Inhibitory Concentration (MIC) values of the three EOs ranged from 0.4 to 0.8% v/v of which nutmeg EO was found most effective.
32812228	1	81	theme	essential	337:345	arg1	EOs					353:355	EOs	353:355	EOs	353:355	The present study was aimed to develop Manihot esculenta and Carrageenan bio-based composite active film functionalized with anise, caraway, and nutmeg essential oils (EOs) and to assess the shelf life of chicken nuggets wrapped with the developed film at refrigeration storage.
32812228	1	81	theme	essential	337:345	arg1	oils					347:350	nutmeg essential oils	330:350	nutmeg essential oils (EOs)	330:356	The present study was aimed to develop Manihot esculenta and Carrageenan bio-based composite active film functionalized with anise, caraway, and nutmeg essential oils (EOs) and to assess the shelf life of chicken nuggets wrapped with the developed film at refrigeration storage.
32812228	11	82	dep	meat	1945:1948	arg1	products					1959:1966	products	1959:1966	products	1959:1966	PRACTICAL APPLICATION: The majority of films used for packaging of meat and meat products are derived from synthetic "plastic" materials.
32812228	3	83	theme	water	714:718	arg1	activity					720:727	water activity	714:727	water activity	714:727	Incorporation of EOs in the film resulted in significant (P ˂ 0.05) decrease in tensile strength and water activity while elongation at break was significantly (P ˂ 0.05) increased.
32812228	0	84	theme	extended	167:174	arg1	storage					176:182	extended storage	167:182	extended storage	167:182	Materialization of novel composite bio-based active edible film functionalized with essential oils on antimicrobial and antioxidative aspect of chicken nuggets during extended storage.
32812228	1	85	theme	chicken	390:396	arg1	nuggets					398:404	chicken nuggets	390:404	chicken nuggets wrapped with the developed film at refrigeration storage	390:461	The present study was aimed to develop Manihot esculenta and Carrageenan bio-based composite active film functionalized with anise, caraway, and nutmeg essential oils (EOs) and to assess the shelf life of chicken nuggets wrapped with the developed film at refrigeration storage.
32812228	2	86	dep	v/v	564:566	arg1	%					562:562	%	562:562	%	562:562	Overall, the Minimum Inhibitory Concentration (MIC) values of the three EOs ranged from 0.4 to 0.8% v/v of which nutmeg EO was found most effective.
32812228	7	87	theme	higher	1434:1439	arg1	activity					1446:1453	significantly (P < 0.05) higher DPPH activity	1409:1453	significantly (P < 0.05) higher DPPH activity	1409:1453	The result indicated that pH, peroxide, free fatty acid (FFA), and thiobarbituric acide (TBA) value of treatments were significantly (P ˂ 0.05) lower than controls however significantly (P < 0.05) higher DPPH activity was observed in all treatments.
32812228	12	88	theme	edible	2114:2119	arg1	films					2121:2125	edible films	2114:2125	edible films	2114:2125	The demerits associated with plastics have eventually led to explore natural alternatives such as edible films.
32812228	6	89	theme	aforementioned	1083:1096	arg1	films					1098:1102	aforementioned films	1083:1102	aforementioned films	1083:1102	Chicken nuggets overwrapped with aforementioned films were stored aerobically at refrigeration temperature (4 ± 1 °C) to evaluate antimicrobial, antioxidant, and sensory characteristics.
32812228	4	90	theme	˃	813:813	arg1	P					811:811	P ˃ 0.05	811:818	P ˃ 0.05	811:818	No significant (P ˃ 0.05) changes in thickness and Water Vapor Transmission Rate (WVTR) were observed.
32812228	7	91	dep	significantly	1409:1421	arg1	P					1424:1424	P < 0.05	1424:1431	P < 0.05	1424:1431	The result indicated that pH, peroxide, free fatty acid (FFA), and thiobarbituric acide (TBA) value of treatments were significantly (P ˂ 0.05) lower than controls however significantly (P < 0.05) higher DPPH activity was observed in all treatments.
32812228	3	92	theme	˂	776:776	arg1	P					774:774	P ˂ 0.05	774:781	P ˂ 0.05	774:781	Incorporation of EOs in the film resulted in significant (P ˂ 0.05) decrease in tensile strength and water activity while elongation at break was significantly (P ˂ 0.05) increased.
32812228	2	93	theme	Inhibitory	485:494	arg1	MIC					511:513	MIC	511:513	MIC	511:513	Overall, the Minimum Inhibitory Concentration (MIC) values of the three EOs ranged from 0.4 to 0.8% v/v of which nutmeg EO was found most effective.
32812228	2	93	theme	Inhibitory	485:494	arg1	Concentration					496:508	Minimum Inhibitory Concentration	477:508	the Minimum Inhibitory Concentration (MIC) values of the three EOs	473:538	Overall, the Minimum Inhibitory Concentration (MIC) values of the three EOs ranged from 0.4 to 0.8% v/v of which nutmeg EO was found most effective.
32812228	12	94	theme	natural	2085:2091	arg1	films					2121:2125	edible films	2114:2125	edible films	2114:2125	The demerits associated with plastics have eventually led to explore natural alternatives such as edible films.
32812228	12	94	theme	natural	2085:2091	arg1	alternatives					2093:2104	natural alternatives	2085:2104	natural alternatives such as edible films	2085:2125	The demerits associated with plastics have eventually led to explore natural alternatives such as edible films.
32812228	6	95	theme	Chicken	1050:1056	arg1	nuggets					1058:1064	Chicken nuggets	1050:1064	Chicken nuggets overwrapped with aforementioned films	1050:1102	Chicken nuggets overwrapped with aforementioned films were stored aerobically at refrigeration temperature (4 ± 1 °C) to evaluate antimicrobial, antioxidant, and sensory characteristics.
32812228	10	96	theme	edible	1775:1780	arg1	film					1792:1795	composite, active edible bio-based film	1757:1795	composite, active edible bio-based film	1757:1795	The application of composite, active edible bio-based film was found proficient in confining product quality attributes throughout storage.
32812228	10	97	theme	quality	1839:1845	arg1	attributes					1847:1856	product quality attributes	1831:1856	product quality attributes	1831:1856	The application of composite, active edible bio-based film was found proficient in confining product quality attributes throughout storage.
32812228	0	98	theme	antimicrobial	102:114	arg1	aspect					134:139	antimicrobial and antioxidative aspect	102:139	aspect	134:139	Materialization of novel composite bio-based active edible film functionalized with essential oils on antimicrobial and antioxidative aspect of chicken nuggets during extended storage.
32812228	8	99	theme	treatment	1600:1608	arg1	groups					1610:1615	treatment groups	1600:1615	treatment groups	1600:1615	The total plate count, psychrophilic count and, yeast and mold count were also significantly (P ˂ 0.01) lower in treatment groups and were within the permissible limits.
32812228	0	100	theme	antioxidative	120:132	arg1	aspect					134:139	antimicrobial and antioxidative aspect	102:139	aspect	134:139	Materialization of novel composite bio-based active edible film functionalized with essential oils on antimicrobial and antioxidative aspect of chicken nuggets during extended storage.
32812228	4	101	theme	Vapor	852:856	arg1	WVTR					877:880	WVTR	877:880	WVTR	877:880	No significant (P ˃ 0.05) changes in thickness and Water Vapor Transmission Rate (WVTR) were observed.
32812228	4	101	theme	Vapor	852:856	arg1	Rate					871:874	Water Vapor Transmission Rate	846:874	Water Vapor Transmission Rate (WVTR)	846:881	No significant (P ˃ 0.05) changes in thickness and Water Vapor Transmission Rate (WVTR) were observed.
32812228	13	102	theme	specific	2204:2211	arg1	properties					2218:2227	specific film properties	2204:2227	specific film properties based on requirements of product-specific packaging conditions	2204:2290	The composite-active bio-based films have a huge potential to be molded for specific film properties based on requirements of product-specific packaging conditions.
32812228	0	103	theme	nuggets	152:158	arg1	aspect					134:139	antimicrobial and antioxidative aspect	102:139	aspect	134:139	Materialization of novel composite bio-based active edible film functionalized with essential oils on antimicrobial and antioxidative aspect of chicken nuggets during extended storage.
32812228	0	104	theme	composite	25:33	arg1	film					59:62	novel composite bio-based active edible film	19:62	novel composite bio-based active edible film	19:62	Materialization of novel composite bio-based active edible film functionalized with essential oils on antimicrobial and antioxidative aspect of chicken nuggets during extended storage.
32812228	8	105	theme	permissible	1637:1647	arg1	limits					1649:1654	the permissible limits	1633:1654	the permissible limits	1633:1654	The total plate count, psychrophilic count and, yeast and mold count were also significantly (P ˂ 0.01) lower in treatment groups and were within the permissible limits.
32812228	5	106	dep	concentration	988:1000	arg1	%					986:986	%	986:986	%	986:986	Based on physicomechanical and sensory evaluation, films incorporated with 0.5, 1, and 1% concentration of anise, nutmeg, and caraway EO were selected.
32812228	13	107	theme	composite-active	2132:2147	arg1	films					2159:2163	The composite-active bio-based films	2128:2163	The composite-active bio-based films	2128:2163	The composite-active bio-based films have a huge potential to be molded for specific film properties based on requirements of product-specific packaging conditions.
32812228	4	108	dep	significant	798:808	arg1	P					811:811	P ˃ 0.05	811:818	P ˃ 0.05	811:818	No significant (P ˃ 0.05) changes in thickness and Water Vapor Transmission Rate (WVTR) were observed.
32812228	11	109	theme	plastic	1996:2002	arg1	materials					2005:2013	synthetic "plastic" materials	1985:2013	synthetic "plastic" materials	1985:2013	PRACTICAL APPLICATION: The majority of films used for packaging of meat and meat products are derived from synthetic "plastic" materials.
32812228	0	110	theme	film	59:62	arg1	Materialization					0:14	Materialization	0:14	Materialization of novel composite bio-based active edible film functionalized with essential oils on antimicrobial and antioxidative aspect of chicken nuggets during extended storage.	0:183	Materialization of novel composite bio-based active edible film functionalized with essential oils on antimicrobial and antioxidative aspect of chicken nuggets during extended storage.
32812228	11	111	theme	synthetic	1985:1993	arg1	materials					2005:2013	synthetic "plastic" materials	1985:2013	synthetic "plastic" materials	1985:2013	PRACTICAL APPLICATION: The majority of films used for packaging of meat and meat products are derived from synthetic "plastic" materials.
32812228	3	112	theme	EOs	630:632	arg1	Incorporation					613:625	Incorporation	613:625	Incorporation of EOs in the film	613:644	Incorporation of EOs in the film resulted in significant (P ˂ 0.05) decrease in tensile strength and water activity while elongation at break was significantly (P ˂ 0.05) increased.
32812228	5	113	theme	caraway	1024:1030	arg1	EO					1032:1033	caraway EO	1024:1033	caraway EO	1024:1033	Based on physicomechanical and sensory evaluation, films incorporated with 0.5, 1, and 1% concentration of anise, nutmeg, and caraway EO were selected.
32812228	7	114	theme	P	1371:1371	arg1	˂					1373:1373	P ˂ 0.05	1371:1378	P ˂ 0.05	1371:1378	The result indicated that pH, peroxide, free fatty acid (FFA), and thiobarbituric acide (TBA) value of treatments were significantly (P ˂ 0.05) lower than controls however significantly (P < 0.05) higher DPPH activity was observed in all treatments.
32812228	1	115	from	storage	455:461	arg1	film					433:436	the developed film	419:436	the developed film at refrigeration storage	419:461	The present study was aimed to develop Manihot esculenta and Carrageenan bio-based composite active film functionalized with anise, caraway, and nutmeg essential oils (EOs) and to assess the shelf life of chicken nuggets wrapped with the developed film at refrigeration storage.
32812228	8	116	theme	plate	1497:1501	arg1	lower					1591:1595	lower	1591:1595	lower	1591:1595	The total plate count, psychrophilic count and, yeast and mold count were also significantly (P ˂ 0.01) lower in treatment groups and were within the permissible limits.
32812228	8	116	theme	plate	1497:1501	arg1	count					1503:1507	The total plate count	1487:1507	The total plate count	1487:1507	The total plate count, psychrophilic count and, yeast and mold count were also significantly (P ˂ 0.01) lower in treatment groups and were within the permissible limits.
32812228	13	117	theme	huge	2172:2175	arg1	potential					2177:2185	a huge potential	2170:2185	a huge potential to be molded for specific film properties based on requirements of product-specific packaging conditions	2170:2290	The composite-active bio-based films have a huge potential to be molded for specific film properties based on requirements of product-specific packaging conditions.
32812228	1	118	theme	bio-based	258:266	arg1	film					285:288	Carrageenan bio-based composite active film	246:288	Carrageenan bio-based composite active film	246:288	The present study was aimed to develop Manihot esculenta and Carrageenan bio-based composite active film functionalized with anise, caraway, and nutmeg essential oils (EOs) and to assess the shelf life of chicken nuggets wrapped with the developed film at refrigeration storage.
32812228	7	119	dep	lower	1381:1385	arg1	˂					1373:1373	P ˂ 0.05	1371:1378	P ˂ 0.05	1371:1378	The result indicated that pH, peroxide, free fatty acid (FFA), and thiobarbituric acide (TBA) value of treatments were significantly (P ˂ 0.05) lower than controls however significantly (P < 0.05) higher DPPH activity was observed in all treatments.
34000101	0	0	theme	lindmaniana	95:105	arg1	Effects					77:83	in Vitro Antihypertensive and Anti-Inflammatory Effects	29:83	in Vitro Antihypertensive and Anti-Inflammatory Effects	29:83	Polyphenolic Composition and in Vitro Antihypertensive and Anti-Inflammatory Effects of Cuphea lindmaniana and Cuphea urbaniana.
34000101	0	0	theme	lindmaniana	95:105	arg1	Composition					13:23	Polyphenolic Composition	0:23	Polyphenolic Composition	0:23	Polyphenolic Composition and in Vitro Antihypertensive and Anti-Inflammatory Effects of Cuphea lindmaniana and Cuphea urbaniana.
34000101	6	1	theme	antichemotactic	795:809	arg1	effect					811:816	antichemotactic effect	795:816	antichemotactic effect	795:816	The extracts displayed antichemotactic effect with a reduction of 100 % of the neutrophil migration, in vitro, in most concentrations.
34000101	2	2	theme	positive	392:399	arg1	ionization					406:415	positive mode ionization	392:415	positive mode ionization	392:415	The extraction was performed ultrasound-assisted, and UHPLC/MS analysis was in positive mode ionization.
34000101	1	3	theme	lindmaniana	272:282	arg1	extracts					253:260	extracts	253:260	extracts of Cuphea lindmaniana	253:282	The present study investigates the chemical composition, anti-inflammatory, and antihypertensive activities, in vitro, from extracts of Cuphea lindmaniana and Cuphea urbaniana leaves.
34000101	0	4	theme	Cuphea	88:93	arg1	lindmaniana					95:105	Cuphea lindmaniana	88:105	Cuphea lindmaniana	88:105	Polyphenolic Composition and in Vitro Antihypertensive and Anti-Inflammatory Effects of Cuphea lindmaniana and Cuphea urbaniana.
34000101	8	5	theme	anti-inflammatory	1091:1107	arg1	potential					1130:1138	in vitro anti-inflammatory and antihypertensive potential	1082:1138	in vitro anti-inflammatory and antihypertensive potential	1082:1138	In conclusion, C. lindmaniana and C. urbaniana extracts contain a rich diversity of flavonoids and display in vitro anti-inflammatory and antihypertensive potential.
34000101	8	6	theme	rich	1041:1044	arg1	diversity					1046:1054	a rich diversity	1039:1054	a rich diversity of flavonoids	1039:1068	In conclusion, C. lindmaniana and C. urbaniana extracts contain a rich diversity of flavonoids and display in vitro anti-inflammatory and antihypertensive potential.
34000101	8	7	theme	antihypertensive	1113:1128	arg1	potential					1130:1138	in vitro anti-inflammatory and antihypertensive potential	1082:1138	in vitro anti-inflammatory and antihypertensive potential	1082:1138	In conclusion, C. lindmaniana and C. urbaniana extracts contain a rich diversity of flavonoids and display in vitro anti-inflammatory and antihypertensive potential.
34000101	0	8	theme	Cuphea	111:116	arg1	urbaniana					118:126	Cuphea urbaniana	111:126	Cuphea urbaniana	111:126	Polyphenolic Composition and in Vitro Antihypertensive and Anti-Inflammatory Effects of Cuphea lindmaniana and Cuphea urbaniana.
34000101	4	9	theme	antihypertensive	583:598	arg1	activity					600:607	The antihypertensive activity	579:607	The antihypertensive activity	579:607	The antihypertensive activity was performed by angiotensin-converting enzyme (ACE) inhibition.
34000101	5	10	theme	proposed	692:699	arg1	compounds					701:709	the nineteen proposed compounds	679:709	the nineteen proposed compounds	679:709	From the nineteen proposed compounds, six of them are described for the first time in this genus.
34000101	9	11	theme	developmental	1225:1237	arg1	products					1245:1252	developmental novel products	1225:1252	developmental novel products with therapeutic actions	1225:1277	Thus, this study could serve as a scientific baseline for further investigation, on developmental novel products with therapeutic actions.
34000101	5	12	theme	first	746:750	arg1	time					752:755	the first time	742:755	the first time in this genus	742:769	From the nineteen proposed compounds, six of them are described for the first time in this genus.
34000101	8	13	theme	flavonoids	1059:1068	arg1	diversity					1046:1054	a rich diversity	1039:1054	a rich diversity of flavonoids	1039:1068	In conclusion, C. lindmaniana and C. urbaniana extracts contain a rich diversity of flavonoids and display in vitro anti-inflammatory and antihypertensive potential.
34000101	1	14	theme	anti-inflammatory	186:202	arg1	composition					173:183	the chemical composition	160:183	the chemical composition	160:183	The present study investigates the chemical composition, anti-inflammatory, and antihypertensive activities, in vitro, from extracts of Cuphea lindmaniana and Cuphea urbaniana leaves.
34000101	1	14	theme	anti-inflammatory	186:202	arg1	activities					226:235	anti-inflammatory, and antihypertensive activities	186:235	anti-inflammatory, and antihypertensive activities	186:235	The present study investigates the chemical composition, anti-inflammatory, and antihypertensive activities, in vitro, from extracts of Cuphea lindmaniana and Cuphea urbaniana leaves.
34000101	0	15	theme	urbaniana	118:126	arg1	Effects					77:83	in Vitro Antihypertensive and Anti-Inflammatory Effects	29:83	in Vitro Antihypertensive and Anti-Inflammatory Effects	29:83	Polyphenolic Composition and in Vitro Antihypertensive and Anti-Inflammatory Effects of Cuphea lindmaniana and Cuphea urbaniana.
34000101	0	15	theme	urbaniana	118:126	arg1	Composition					13:23	Polyphenolic Composition	0:23	Polyphenolic Composition	0:23	Polyphenolic Composition and in Vitro Antihypertensive and Anti-Inflammatory Effects of Cuphea lindmaniana and Cuphea urbaniana.
34000101	3	16	theme	extracts	456:463	arg1	miquelianin					469:479	miquelianin	469:479	miquelianin	469:479	The anti-inflammatory activity of the extracts and miquelianin were assayed at concentrations 0.001-10 μg/mL by chemotaxis on rat polymorphonuclear neutrophils.
34000101	3	16	theme	extracts	456:463	arg1	activity					440:447	The anti-inflammatory activity	418:447	The anti-inflammatory activity of the extracts	418:463	The anti-inflammatory activity of the extracts and miquelianin were assayed at concentrations 0.001-10 μg/mL by chemotaxis on rat polymorphonuclear neutrophils.
34000101	6	17	theme	migration	862:870	arg1	in vitro					873:880	in vitro	873:880	in vitro	873:880	The extracts displayed antichemotactic effect with a reduction of 100 % of the neutrophil migration, in vitro, in most concentrations.
34000101	6	17	theme	migration	862:870	arg1	migration					862:870	the neutrophil migration	847:870	the neutrophil migration	847:870	The extracts displayed antichemotactic effect with a reduction of 100 % of the neutrophil migration, in vitro, in most concentrations.
34000101	6	17	theme	migration	862:870	arg1	%					842:842	100 %	838:842	100 % of the neutrophil migration, in vitro,	838:881	The extracts displayed antichemotactic effect with a reduction of 100 % of the neutrophil migration, in vitro, in most concentrations.
34000101	3	18	theme	anti-inflammatory	422:438	arg1	activity					440:447	The anti-inflammatory activity	418:447	The anti-inflammatory activity of the extracts	418:463	The anti-inflammatory activity of the extracts and miquelianin were assayed at concentrations 0.001-10 μg/mL by chemotaxis on rat polymorphonuclear neutrophils.
34000101	5	19	theme	them	719:722	arg1	them					719:722	them	719:722	them	719:722	From the nineteen proposed compounds, six of them are described for the first time in this genus.
34000101	5	19	theme	them	719:722	arg1	six					712:714	six	712:714	six	712:714	From the nineteen proposed compounds, six of them are described for the first time in this genus.
34000101	6	20	theme	neutrophil	851:860	arg1	in vitro					873:880	in vitro	873:880	in vitro	873:880	The extracts displayed antichemotactic effect with a reduction of 100 % of the neutrophil migration, in vitro, in most concentrations.
34000101	6	20	theme	neutrophil	851:860	arg1	migration					862:870	the neutrophil migration	847:870	the neutrophil migration	847:870	The extracts displayed antichemotactic effect with a reduction of 100 % of the neutrophil migration, in vitro, in most concentrations.
34000101	0	21	theme	Polyphenolic	0:11	arg1	Composition					13:23	Polyphenolic Composition	0:23	Polyphenolic Composition	0:23	Polyphenolic Composition and in Vitro Antihypertensive and Anti-Inflammatory Effects of Cuphea lindmaniana and Cuphea urbaniana.
34000101	8	22	theme	in vitro	1082:1089	arg1	potential					1130:1138	in vitro anti-inflammatory and antihypertensive potential	1082:1138	in vitro anti-inflammatory and antihypertensive potential	1082:1138	In conclusion, C. lindmaniana and C. urbaniana extracts contain a rich diversity of flavonoids and display in vitro anti-inflammatory and antihypertensive potential.
34000101	1	23	theme	antihypertensive	209:224	arg1	composition					173:183	the chemical composition	160:183	the chemical composition	160:183	The present study investigates the chemical composition, anti-inflammatory, and antihypertensive activities, in vitro, from extracts of Cuphea lindmaniana and Cuphea urbaniana leaves.
34000101	1	23	theme	antihypertensive	209:224	arg1	activities					226:235	anti-inflammatory, and antihypertensive activities	186:235	anti-inflammatory, and antihypertensive activities	186:235	The present study investigates the chemical composition, anti-inflammatory, and antihypertensive activities, in vitro, from extracts of Cuphea lindmaniana and Cuphea urbaniana leaves.
34000101	0	24	theme	in	29:30	arg1	Effects					77:83	in Vitro Antihypertensive and Anti-Inflammatory Effects	29:83	in Vitro Antihypertensive and Anti-Inflammatory Effects	29:83	Polyphenolic Composition and in Vitro Antihypertensive and Anti-Inflammatory Effects of Cuphea lindmaniana and Cuphea urbaniana.
34000101	4	25	theme	enzyme	649:654	arg1	inhibition					662:671	angiotensin-converting enzyme (ACE) inhibition	626:671	angiotensin-converting enzyme (ACE) inhibition	626:671	The antihypertensive activity was performed by angiotensin-converting enzyme (ACE) inhibition.
34000101	9	26	theme	scientific	1175:1184	arg1	study					1152:1156	this study	1147:1156	this study	1147:1156	Thus, this study could serve as a scientific baseline for further investigation, on developmental novel products with therapeutic actions.
34000101	9	26	theme	scientific	1175:1184	arg1	baseline					1186:1193	a scientific baseline	1173:1193	a scientific baseline for further investigation	1173:1219	Thus, this study could serve as a scientific baseline for further investigation, on developmental novel products with therapeutic actions.
34000101	0	27	theme	Antihypertensive	38:53	arg1	Effects					77:83	in Vitro Antihypertensive and Anti-Inflammatory Effects	29:83	in Vitro Antihypertensive and Anti-Inflammatory Effects	29:83	Polyphenolic Composition and in Vitro Antihypertensive and Anti-Inflammatory Effects of Cuphea lindmaniana and Cuphea urbaniana.
34000101	6	28	from	reduction	825:833	arg1	concentrations					891:904	most concentrations	886:904	most concentrations	886:904	The extracts displayed antichemotactic effect with a reduction of 100 % of the neutrophil migration, in vitro, in most concentrations.
34000101	9	29	theme	therapeutic	1259:1269	arg1	actions					1271:1277	therapeutic actions	1259:1277	therapeutic actions	1259:1277	Thus, this study could serve as a scientific baseline for further investigation, on developmental novel products with therapeutic actions.
34000101	6	30	theme	%	842:842	arg1	reduction					825:833	a reduction	823:833	a reduction of 100 % of the neutrophil migration, in vitro, in most concentrations	823:904	The extracts displayed antichemotactic effect with a reduction of 100 % of the neutrophil migration, in vitro, in most concentrations.
34000101	0	31	theme	Anti-Inflammatory	59:75	arg1	Effects					77:83	in Vitro Antihypertensive and Anti-Inflammatory Effects	29:83	in Vitro Antihypertensive and Anti-Inflammatory Effects	29:83	Polyphenolic Composition and in Vitro Antihypertensive and Anti-Inflammatory Effects of Cuphea lindmaniana and Cuphea urbaniana.
34000101	8	32	contain	contain	1031:1037	arg1	conclusion					978:987	conclusion	978:987	conclusion	978:987	In conclusion, C. lindmaniana and C. urbaniana extracts contain a rich diversity of flavonoids and display in vitro anti-inflammatory and antihypertensive potential.
34000101	8	32	contain	contain	1031:1037	arg1	extracts					1022:1029	C. lindmaniana and C. urbaniana extracts	990:1029	C. lindmaniana and C. urbaniana extracts	990:1029	In conclusion, C. lindmaniana and C. urbaniana extracts contain a rich diversity of flavonoids and display in vitro anti-inflammatory and antihypertensive potential.
34000101	8	32	contain	contain	1031:1037	arg2	extracts					1022:1029	C. lindmaniana and C. urbaniana extracts	990:1029	C. lindmaniana and C. urbaniana extracts	990:1029	In conclusion, C. lindmaniana and C. urbaniana extracts contain a rich diversity of flavonoids and display in vitro anti-inflammatory and antihypertensive potential.
34000101	8	32	contain	contain	1031:1037	arg2	diversity					1046:1054	a rich diversity	1039:1054	a rich diversity of flavonoids	1039:1068	In conclusion, C. lindmaniana and C. urbaniana extracts contain a rich diversity of flavonoids and display in vitro anti-inflammatory and antihypertensive potential.
34000101	3	33	theme	polymorphonuclear	548:564	arg1	neutrophils					566:576	rat polymorphonuclear neutrophils	544:576	rat polymorphonuclear neutrophils	544:576	The anti-inflammatory activity of the extracts and miquelianin were assayed at concentrations 0.001-10 μg/mL by chemotaxis on rat polymorphonuclear neutrophils.
34000101	4	34	theme	angiotensin-converting	626:647	arg1	ACE					657:659	ACE	657:659	ACE	657:659	The antihypertensive activity was performed by angiotensin-converting enzyme (ACE) inhibition.
34000101	4	34	theme	angiotensin-converting	626:647	arg1	enzyme					649:654	angiotensin-converting enzyme	626:654	angiotensin-converting enzyme (ACE) inhibition	626:671	The antihypertensive activity was performed by angiotensin-converting enzyme (ACE) inhibition.
34000101	1	35	theme	chemical	164:171	arg1	in vitro					238:245	in vitro	238:245	in vitro	238:245	The present study investigates the chemical composition, anti-inflammatory, and antihypertensive activities, in vitro, from extracts of Cuphea lindmaniana and Cuphea urbaniana leaves.
34000101	1	35	theme	chemical	164:171	arg1	composition					173:183	the chemical composition	160:183	the chemical composition	160:183	The present study investigates the chemical composition, anti-inflammatory, and antihypertensive activities, in vitro, from extracts of Cuphea lindmaniana and Cuphea urbaniana leaves.
34000101	1	35	theme	chemical	164:171	arg1	activities					226:235	anti-inflammatory, and antihypertensive activities	186:235	anti-inflammatory, and antihypertensive activities	186:235	The present study investigates the chemical composition, anti-inflammatory, and antihypertensive activities, in vitro, from extracts of Cuphea lindmaniana and Cuphea urbaniana leaves.
34000101	1	36	theme	present	133:139	arg1	study					141:145	The present study	129:145	The present study	129:145	The present study investigates the chemical composition, anti-inflammatory, and antihypertensive activities, in vitro, from extracts of Cuphea lindmaniana and Cuphea urbaniana leaves.
34000101	0	37	dep	in	29:30	arg1	Vitro					32:36	Vitro	32:36	Vitro	32:36	Polyphenolic Composition and in Vitro Antihypertensive and Anti-Inflammatory Effects of Cuphea lindmaniana and Cuphea urbaniana.
34000101	8	38	theme	lindmaniana	993:1003	arg1	extracts					1022:1029	C. lindmaniana and C. urbaniana extracts	990:1029	C. lindmaniana and C. urbaniana extracts	990:1029	In conclusion, C. lindmaniana and C. urbaniana extracts contain a rich diversity of flavonoids and display in vitro anti-inflammatory and antihypertensive potential.
34000101	2	39	theme	UHPLC/MS	367:374	arg1	analysis					376:383	UHPLC/MS analysis	367:383	UHPLC/MS analysis	367:383	The extraction was performed ultrasound-assisted, and UHPLC/MS analysis was in positive mode ionization.
34000101	9	40	with	products	1245:1252	arg1	actions					1271:1277	therapeutic actions	1259:1277	therapeutic actions	1259:1277	Thus, this study could serve as a scientific baseline for further investigation, on developmental novel products with therapeutic actions.
34000101	1	41	from	extracts	253:260	arg1	in vitro					238:245	in vitro	238:245	in vitro	238:245	The present study investigates the chemical composition, anti-inflammatory, and antihypertensive activities, in vitro, from extracts of Cuphea lindmaniana and Cuphea urbaniana leaves.
34000101	1	41	from	extracts	253:260	arg1	composition					173:183	the chemical composition	160:183	the chemical composition	160:183	The present study investigates the chemical composition, anti-inflammatory, and antihypertensive activities, in vitro, from extracts of Cuphea lindmaniana and Cuphea urbaniana leaves.
34000101	1	41	from	extracts	253:260	arg1	activities					226:235	anti-inflammatory, and antihypertensive activities	186:235	anti-inflammatory, and antihypertensive activities	186:235	The present study investigates the chemical composition, anti-inflammatory, and antihypertensive activities, in vitro, from extracts of Cuphea lindmaniana and Cuphea urbaniana leaves.
34000101	9	42	theme	further	1199:1205	arg1	investigation					1207:1219	further investigation	1199:1219	further investigation	1199:1219	Thus, this study could serve as a scientific baseline for further investigation, on developmental novel products with therapeutic actions.
34000101	8	43	theme	urbaniana	1012:1020	arg1	extracts					1022:1029	C. lindmaniana and C. urbaniana extracts	990:1029	C. lindmaniana and C. urbaniana extracts	990:1029	In conclusion, C. lindmaniana and C. urbaniana extracts contain a rich diversity of flavonoids and display in vitro anti-inflammatory and antihypertensive potential.
34000101	3	44	theme	rat	544:546	arg1	neutrophils					566:576	rat polymorphonuclear neutrophils	544:576	rat polymorphonuclear neutrophils	544:576	The anti-inflammatory activity of the extracts and miquelianin were assayed at concentrations 0.001-10 μg/mL by chemotaxis on rat polymorphonuclear neutrophils.
34000101	6	45	theme	most	886:889	arg1	concentrations					891:904	most concentrations	886:904	most concentrations	886:904	The extracts displayed antichemotactic effect with a reduction of 100 % of the neutrophil migration, in vitro, in most concentrations.
34000101	9	46	theme	novel	1239:1243	arg1	products					1245:1252	developmental novel products	1225:1252	developmental novel products with therapeutic actions	1225:1277	Thus, this study could serve as a scientific baseline for further investigation, on developmental novel products with therapeutic actions.
34000101	5	47	from	time	752:755	arg1	genus					765:769	this genus	760:769	this genus	760:769	From the nineteen proposed compounds, six of them are described for the first time in this genus.
34000101	2	48	theme	mode	401:404	arg1	ionization					406:415	positive mode ionization	392:415	positive mode ionization	392:415	The extraction was performed ultrasound-assisted, and UHPLC/MS analysis was in positive mode ionization.
34000101	1	49	theme	Cuphea	265:270	arg1	lindmaniana					272:282	Cuphea lindmaniana	265:282	Cuphea lindmaniana	265:282	The present study investigates the chemical composition, anti-inflammatory, and antihypertensive activities, in vitro, from extracts of Cuphea lindmaniana and Cuphea urbaniana leaves.
34544272	6	0	theme	wall	963:966	arg1	antibiotics					978:988	cell wall targeting antibiotics	958:988	cell wall targeting antibiotics	958:988	Genetic analysis and cell wall targeting antibiotics reveal that the mechanism of unipolar growth is conserved in Sinorhizobium and Brucella.
34544272	9	1	theme	cell	1499:1502	arg1	biosynthesis					1509:1520	cell wall biosynthesis	1499:1520	cell wall biosynthesis	1499:1520	It is increasingly clear that rod-shaped bacteria use a diverse array of growth strategies with distinct spatial zones of cell wall biosynthesis, including lateral elongation, unipolar growth, bipolar elongation, and medial elongation.
34544272	3	2	theme	penicillin-binding	516:533	arg1	proteins					535:542	penicillin-binding proteins	516:542	penicillin-binding proteins in A. tumefaciens	516:560	The development of fluorescent d-amino acid dipeptide (FDAAD) probes, which are incorporated into peptidoglycan by penicillin-binding proteins in A. tumefaciens, enabled us to monitor changes in growth patterns in the mutants.
34544272	9	3	theme	biosynthesis	1509:1520	arg1	elongation					1601:1610	medial elongation	1594:1610	medial elongation	1594:1610	It is increasingly clear that rod-shaped bacteria use a diverse array of growth strategies with distinct spatial zones of cell wall biosynthesis, including lateral elongation, unipolar growth, bipolar elongation, and medial elongation.
34544272	9	3	theme	biosynthesis	1509:1520	arg1	elongation					1541:1550	lateral elongation	1533:1550	lateral elongation	1533:1550	It is increasingly clear that rod-shaped bacteria use a diverse array of growth strategies with distinct spatial zones of cell wall biosynthesis, including lateral elongation, unipolar growth, bipolar elongation, and medial elongation.
34544272	9	3	theme	biosynthesis	1509:1520	arg1	zones					1490:1494	distinct spatial zones	1473:1494	distinct spatial zones	1473:1494	It is increasingly clear that rod-shaped bacteria use a diverse array of growth strategies with distinct spatial zones of cell wall biosynthesis, including lateral elongation, unipolar growth, bipolar elongation, and medial elongation.
34544272	9	3	theme	biosynthesis	1509:1520	arg1	growth					1562:1567	unipolar growth	1553:1567	unipolar growth	1553:1567	It is increasingly clear that rod-shaped bacteria use a diverse array of growth strategies with distinct spatial zones of cell wall biosynthesis, including lateral elongation, unipolar growth, bipolar elongation, and medial elongation.
34544272	9	3	theme	biosynthesis	1509:1520	arg1	elongation					1578:1587	bipolar elongation	1570:1587	bipolar elongation	1570:1587	It is increasingly clear that rod-shaped bacteria use a diverse array of growth strategies with distinct spatial zones of cell wall biosynthesis, including lateral elongation, unipolar growth, bipolar elongation, and medial elongation.
34544272	8	4	theme	bacterial	1251:1259	arg1	wall					1266:1269	the bacterial cell wall	1247:1269	the bacterial cell wall	1247:1269	IMPORTANCE While the structure and function of the bacterial cell wall are well conserved, the mechanisms responsible for cell wall biosynthesis during elongation are variable.
34544272	6	5	theme	Genetic	937:943	arg1	analysis					945:952	Genetic analysis	937:952	Genetic analysis	937:952	Genetic analysis and cell wall targeting antibiotics reveal that the mechanism of unipolar growth is conserved in Sinorhizobium and Brucella.
34544272	8	6	theme	wall	1266:1269	arg1	structure					1221:1229	structure	1221:1229	structure	1221:1229	IMPORTANCE While the structure and function of the bacterial cell wall are well conserved, the mechanisms responsible for cell wall biosynthesis during elongation are variable.
34544272	8	6	theme	wall	1266:1269	arg1	function					1235:1242	function	1235:1242	function	1235:1242	IMPORTANCE While the structure and function of the bacterial cell wall are well conserved, the mechanisms responsible for cell wall biosynthesis during elongation are variable.
34544272	4	7	theme	compositional	688:700	arg1	analysis					702:709	peptidoglycan compositional analysis	674:709	peptidoglycan compositional analysis	674:709	Use of these fluorescent cell wall probes and peptidoglycan compositional analysis demonstrate that a single class A penicillin-binding protein is essential for polar peptidoglycan synthesis.
34544272	13	8	theme	attractive	2061:2070	arg1	targets					2072:2078	attractive targets	2061:2078	attractive targets for biocontrol of pathogenic Rhizobiales	2061:2119	Thus, these enzymes may serve as attractive targets for biocontrol of pathogenic Rhizobiales.
34544272	13	8	theme	attractive	2061:2070	arg1	enzymes					2040:2046	these enzymes	2034:2046	these enzymes	2034:2046	Thus, these enzymes may serve as attractive targets for biocontrol of pathogenic Rhizobiales.
34544272	1	9	theme	canonical	192:200	arg1	complex					206:212	the canonical Rod complex	188:212	the canonical Rod complex	188:212	Members of the Rhizobiales are polarly growing bacteria that lack homologs of the canonical Rod complex.
34544272	6	10	theme	unipolar	1019:1026	arg1	growth					1028:1033	unipolar growth	1019:1033	unipolar growth	1019:1033	Genetic analysis and cell wall targeting antibiotics reveal that the mechanism of unipolar growth is conserved in Sinorhizobium and Brucella.
34544272	4	11	theme	fluorescent	641:651	arg1	probes					663:668	these fluorescent cell wall probes	635:668	these fluorescent cell wall probes	635:668	Use of these fluorescent cell wall probes and peptidoglycan compositional analysis demonstrate that a single class A penicillin-binding protein is essential for polar peptidoglycan synthesis.
34544272	9	12	theme	unipolar	1553:1560	arg1	growth					1562:1567	unipolar growth	1553:1567	unipolar growth	1553:1567	It is increasingly clear that rod-shaped bacteria use a diverse array of growth strategies with distinct spatial zones of cell wall biosynthesis, including lateral elongation, unipolar growth, bipolar elongation, and medial elongation.
34544272	4	13	theme	wall	658:661	arg1	probes					663:668	these fluorescent cell wall probes	635:668	these fluorescent cell wall probes	635:668	Use of these fluorescent cell wall probes and peptidoglycan compositional analysis demonstrate that a single class A penicillin-binding protein is essential for polar peptidoglycan synthesis.
34544272	7	14	theme	peptidoglycan	1121:1133	arg1	biosynthesis					1135:1146	unipolar peptidoglycan biosynthesis	1112:1146	unipolar peptidoglycan biosynthesis employed by the Rhizobiales during cell elongation	1112:1197	This work provides insights into unipolar peptidoglycan biosynthesis employed by the Rhizobiales during cell elongation.
34544272	5	15	theme	wall	880:883	arg1	synthesis					885:893	cell wall synthesis	875:893	cell wall synthesis	875:893	Furthermore, we find evidence of an additional mode of cell wall synthesis that requires ld-transpeptidase activity.
34544272	10	16	theme	model	1703:1707	arg1	organisms					1709:1717	model organisms	1703:1717	model organisms exhibiting lateral elongation	1703:1747	Yet the vast majority of our understanding regarding bacterial elongation is derived from model organisms exhibiting lateral elongation.
34544272	1	17	theme	Rhizobiales	125:135	arg1	bacteria					157:164	polarly growing bacteria	141:164	polarly growing bacteria that lack homologs of the canonical Rod complex	141:212	Members of the Rhizobiales are polarly growing bacteria that lack homologs of the canonical Rod complex.
34544272	1	17	theme	Rhizobiales	125:135	arg1	Members					110:116	Members	110:116	Members of the Rhizobiales	110:135	Members of the Rhizobiales are polarly growing bacteria that lack homologs of the canonical Rod complex.
34544272	4	18	theme	penicillin-binding	745:762	arg1	protein					764:770	A penicillin-binding protein	743:770	a single class A penicillin-binding protein	728:770	Use of these fluorescent cell wall probes and peptidoglycan compositional analysis demonstrate that a single class A penicillin-binding protein is essential for polar peptidoglycan synthesis.
34544272	11	19	theme	related	1863:1869	arg1	bacteria					1871:1878	related bacteria	1863:1878	related bacteria	1863:1878	Here, we explore the role of penicillin-binding proteins in unipolar elongation of Agrobacterium tumefaciens and related bacteria within the Rhizobiales.
34544272	2	20	theme	wall	267:270	arg1	synthesis					272:280	polar cell wall synthesis	256:280	polar cell wall synthesis	256:280	To investigate the mechanisms underlying polar cell wall synthesis, we systematically probed the function of cell wall synthesis enzymes in the plant pathogen Agrobacterium tumefaciens.
34544272	11	21	theme	tumefaciens	1847:1857	arg1	elongation					1819:1828	unipolar elongation	1810:1828	unipolar elongation of Agrobacterium tumefaciens and related bacteria	1810:1878	Here, we explore the role of penicillin-binding proteins in unipolar elongation of Agrobacterium tumefaciens and related bacteria within the Rhizobiales.
34544272	0	22	theme	Essential	64:72	arg1	Protein					101:107	an Essential Class A Penicillin-Binding Protein	61:107	an Essential Class A Penicillin-Binding Protein	61:107	Unipolar Peptidoglycan Synthesis in the Rhizobiales Requires an Essential Class A Penicillin-Binding Protein.
34544272	2	23	theme	polar	256:260	arg1	synthesis					272:280	polar cell wall synthesis	256:280	polar cell wall synthesis	256:280	To investigate the mechanisms underlying polar cell wall synthesis, we systematically probed the function of cell wall synthesis enzymes in the plant pathogen Agrobacterium tumefaciens.
34544272	2	24	from	function	312:319	arg1	tumefaciens					388:398	the plant pathogen Agrobacterium tumefaciens	355:398	the plant pathogen Agrobacterium tumefaciens	355:398	To investigate the mechanisms underlying polar cell wall synthesis, we systematically probed the function of cell wall synthesis enzymes in the plant pathogen Agrobacterium tumefaciens.
34544272	0	25	from	Synthesis	23:31	arg1	Rhizobiales					40:50	the Rhizobiales	36:50	the Rhizobiales	36:50	Unipolar Peptidoglycan Synthesis in the Rhizobiales Requires an Essential Class A Penicillin-Binding Protein.
34544272	0	26	theme	A	80:80	arg1	Protein					101:107	an Essential Class A Penicillin-Binding Protein	61:107	an Essential Class A Penicillin-Binding Protein	61:107	Unipolar Peptidoglycan Synthesis in the Rhizobiales Requires an Essential Class A Penicillin-Binding Protein.
34544272	4	27	theme	peptidoglycan	795:807	arg1	synthesis					809:817	polar peptidoglycan synthesis	789:817	polar peptidoglycan synthesis	789:817	Use of these fluorescent cell wall probes and peptidoglycan compositional analysis demonstrate that a single class A penicillin-binding protein is essential for polar peptidoglycan synthesis.
34544272	3	28	theme	FDAAD	456:460	arg1	probes					463:468	fluorescent d-amino acid dipeptide (FDAAD) probes	420:468	fluorescent d-amino acid dipeptide (FDAAD) probes	420:468	The development of fluorescent d-amino acid dipeptide (FDAAD) probes, which are incorporated into peptidoglycan by penicillin-binding proteins in A. tumefaciens, enabled us to monitor changes in growth patterns in the mutants.
34544272	12	29	theme	unipolar	2011:2018	arg1	growth					2020:2025	unipolar growth	2011:2025	unipolar growth	2011:2025	Our findings suggest that penicillin-binding protein 1a, along with a subset of ld-transpeptidases, drives unipolar growth.
34544272	3	30	theme	dipeptide	445:453	arg1	probes					463:468	fluorescent d-amino acid dipeptide (FDAAD) probes	420:468	fluorescent d-amino acid dipeptide (FDAAD) probes	420:468	The development of fluorescent d-amino acid dipeptide (FDAAD) probes, which are incorporated into peptidoglycan by penicillin-binding proteins in A. tumefaciens, enabled us to monitor changes in growth patterns in the mutants.
34544272	0	31	theme	Unipolar	0:7	arg1	Synthesis					23:31	Unipolar Peptidoglycan Synthesis	0:31	Unipolar Peptidoglycan Synthesis in the Rhizobiales	0:50	Unipolar Peptidoglycan Synthesis in the Rhizobiales Requires an Essential Class A Penicillin-Binding Protein.
34544272	5	32	theme	additional	856:865	arg1	mode					867:870	an additional mode	853:870	an additional mode of cell wall synthesis that requires ld-transpeptidase activity	853:934	Furthermore, we find evidence of an additional mode of cell wall synthesis that requires ld-transpeptidase activity.
34544272	1	33	theme	growing	149:155	arg1	bacteria					157:164	polarly growing bacteria	141:164	polarly growing bacteria that lack homologs of the canonical Rod complex	141:212	Members of the Rhizobiales are polarly growing bacteria that lack homologs of the canonical Rod complex.
34544272	1	33	theme	growing	149:155	arg1	Members					110:116	Members	110:116	Members of the Rhizobiales	110:135	Members of the Rhizobiales are polarly growing bacteria that lack homologs of the canonical Rod complex.
34544272	3	34	theme	d-amino	432:438	arg1	probes					463:468	fluorescent d-amino acid dipeptide (FDAAD) probes	420:468	fluorescent d-amino acid dipeptide (FDAAD) probes	420:468	The development of fluorescent d-amino acid dipeptide (FDAAD) probes, which are incorporated into peptidoglycan by penicillin-binding proteins in A. tumefaciens, enabled us to monitor changes in growth patterns in the mutants.
34544272	3	35	theme	probes	463:468	arg1	development					405:415	The development	401:415	The development	401:415	The development of fluorescent d-amino acid dipeptide (FDAAD) probes, which are incorporated into peptidoglycan by penicillin-binding proteins in A. tumefaciens, enabled us to monitor changes in growth patterns in the mutants.
34544272	12	36	theme	protein	1949:1955	arg1	1a					1957:1958	penicillin-binding protein 1a	1930:1958	penicillin-binding protein 1a	1930:1958	Our findings suggest that penicillin-binding protein 1a, along with a subset of ld-transpeptidases, drives unipolar growth.
34544272	11	37	theme	penicillin-binding	1779:1796	arg1	proteins					1798:1805	penicillin-binding proteins	1779:1805	penicillin-binding proteins	1779:1805	Here, we explore the role of penicillin-binding proteins in unipolar elongation of Agrobacterium tumefaciens and related bacteria within the Rhizobiales.
34544272	9	38	theme	growth	1450:1455	arg1	strategies					1457:1466	growth strategies	1450:1466	growth strategies	1450:1466	It is increasingly clear that rod-shaped bacteria use a diverse array of growth strategies with distinct spatial zones of cell wall biosynthesis, including lateral elongation, unipolar growth, bipolar elongation, and medial elongation.
34544272	11	39	theme	Agrobacterium	1833:1845	arg1	tumefaciens					1847:1857	Agrobacterium tumefaciens	1833:1857	Agrobacterium tumefaciens	1833:1857	Here, we explore the role of penicillin-binding proteins in unipolar elongation of Agrobacterium tumefaciens and related bacteria within the Rhizobiales.
34544272	2	40	theme	synthesis	334:342	arg1	enzymes					344:350	cell wall synthesis enzymes	324:350	cell wall synthesis enzymes	324:350	To investigate the mechanisms underlying polar cell wall synthesis, we systematically probed the function of cell wall synthesis enzymes in the plant pathogen Agrobacterium tumefaciens.
34544272	2	41	theme	Agrobacterium	374:386	arg1	tumefaciens					388:398	the plant pathogen Agrobacterium tumefaciens	355:398	the plant pathogen Agrobacterium tumefaciens	355:398	To investigate the mechanisms underlying polar cell wall synthesis, we systematically probed the function of cell wall synthesis enzymes in the plant pathogen Agrobacterium tumefaciens.
34544272	8	42	theme	responsible	1306:1316	arg1	variable					1367:1374	variable	1367:1374	variable	1367:1374	IMPORTANCE While the structure and function of the bacterial cell wall are well conserved, the mechanisms responsible for cell wall biosynthesis during elongation are variable.
34544272	8	42	theme	responsible	1306:1316	arg1	mechanisms					1295:1304	the mechanisms	1291:1304	the mechanisms responsible for cell wall biosynthesis during elongation	1291:1361	IMPORTANCE While the structure and function of the bacterial cell wall are well conserved, the mechanisms responsible for cell wall biosynthesis during elongation are variable.
34544272	9	43	theme	spatial	1482:1488	arg1	elongation					1601:1610	medial elongation	1594:1610	medial elongation	1594:1610	It is increasingly clear that rod-shaped bacteria use a diverse array of growth strategies with distinct spatial zones of cell wall biosynthesis, including lateral elongation, unipolar growth, bipolar elongation, and medial elongation.
34544272	9	43	theme	spatial	1482:1488	arg1	elongation					1541:1550	lateral elongation	1533:1550	lateral elongation	1533:1550	It is increasingly clear that rod-shaped bacteria use a diverse array of growth strategies with distinct spatial zones of cell wall biosynthesis, including lateral elongation, unipolar growth, bipolar elongation, and medial elongation.
34544272	9	43	theme	spatial	1482:1488	arg1	zones					1490:1494	distinct spatial zones	1473:1494	distinct spatial zones	1473:1494	It is increasingly clear that rod-shaped bacteria use a diverse array of growth strategies with distinct spatial zones of cell wall biosynthesis, including lateral elongation, unipolar growth, bipolar elongation, and medial elongation.
34544272	9	43	theme	spatial	1482:1488	arg1	growth					1562:1567	unipolar growth	1553:1567	unipolar growth	1553:1567	It is increasingly clear that rod-shaped bacteria use a diverse array of growth strategies with distinct spatial zones of cell wall biosynthesis, including lateral elongation, unipolar growth, bipolar elongation, and medial elongation.
34544272	9	43	theme	spatial	1482:1488	arg1	elongation					1578:1587	bipolar elongation	1570:1587	bipolar elongation	1570:1587	It is increasingly clear that rod-shaped bacteria use a diverse array of growth strategies with distinct spatial zones of cell wall biosynthesis, including lateral elongation, unipolar growth, bipolar elongation, and medial elongation.
34544272	6	44	theme	targeting	968:976	arg1	antibiotics					978:988	cell wall targeting antibiotics	958:988	cell wall targeting antibiotics	958:988	Genetic analysis and cell wall targeting antibiotics reveal that the mechanism of unipolar growth is conserved in Sinorhizobium and Brucella.
34544272	10	45	theme	vast	1621:1624	arg1	majority					1626:1633	the vast majority	1617:1633	the vast majority of our understanding regarding bacterial elongation	1617:1685	Yet the vast majority of our understanding regarding bacterial elongation is derived from model organisms exhibiting lateral elongation.
34544272	2	46	theme	plant	359:363	arg1	tumefaciens					388:398	the plant pathogen Agrobacterium tumefaciens	355:398	the plant pathogen Agrobacterium tumefaciens	355:398	To investigate the mechanisms underlying polar cell wall synthesis, we systematically probed the function of cell wall synthesis enzymes in the plant pathogen Agrobacterium tumefaciens.
34544272	6	47	theme	cell	958:961	arg1	antibiotics					978:988	cell wall targeting antibiotics	958:988	cell wall targeting antibiotics	958:988	Genetic analysis and cell wall targeting antibiotics reveal that the mechanism of unipolar growth is conserved in Sinorhizobium and Brucella.
34544272	10	48	theme	bacterial	1666:1674	arg1	elongation					1676:1685	bacterial elongation	1666:1685	bacterial elongation	1666:1685	Yet the vast majority of our understanding regarding bacterial elongation is derived from model organisms exhibiting lateral elongation.
34544272	7	49	theme	cell	1183:1186	arg1	elongation					1188:1197	cell elongation	1183:1197	cell elongation	1183:1197	This work provides insights into unipolar peptidoglycan biosynthesis employed by the Rhizobiales during cell elongation.
34544272	3	50	from	proteins	535:542	arg1	tumefaciens					550:560	A. tumefaciens	547:560	A. tumefaciens	547:560	The development of fluorescent d-amino acid dipeptide (FDAAD) probes, which are incorporated into peptidoglycan by penicillin-binding proteins in A. tumefaciens, enabled us to monitor changes in growth patterns in the mutants.
34544272	9	51	theme	wall	1504:1507	arg1	biosynthesis					1509:1520	cell wall biosynthesis	1499:1520	cell wall biosynthesis	1499:1520	It is increasingly clear that rod-shaped bacteria use a diverse array of growth strategies with distinct spatial zones of cell wall biosynthesis, including lateral elongation, unipolar growth, bipolar elongation, and medial elongation.
34544272	10	52	theme	understanding	1642:1654	arg1	majority					1626:1633	the vast majority	1617:1633	the vast majority of our understanding regarding bacterial elongation	1617:1685	Yet the vast majority of our understanding regarding bacterial elongation is derived from model organisms exhibiting lateral elongation.
34544272	13	53	theme	pathogenic	2098:2107	arg1	Rhizobiales					2109:2119	pathogenic Rhizobiales	2098:2119	pathogenic Rhizobiales	2098:2119	Thus, these enzymes may serve as attractive targets for biocontrol of pathogenic Rhizobiales.
34544272	8	54	theme	wall	1327:1330	arg1	biosynthesis					1332:1343	cell wall biosynthesis	1322:1343	cell wall biosynthesis during elongation	1322:1361	IMPORTANCE While the structure and function of the bacterial cell wall are well conserved, the mechanisms responsible for cell wall biosynthesis during elongation are variable.
34544272	5	55	theme	synthesis	885:893	arg1	mode					867:870	an additional mode	853:870	an additional mode of cell wall synthesis that requires ld-transpeptidase activity	853:934	Furthermore, we find evidence of an additional mode of cell wall synthesis that requires ld-transpeptidase activity.
34544272	4	56	theme	probes	663:668	arg1	analysis					702:709	peptidoglycan compositional analysis	674:709	peptidoglycan compositional analysis	674:709	Use of these fluorescent cell wall probes and peptidoglycan compositional analysis demonstrate that a single class A penicillin-binding protein is essential for polar peptidoglycan synthesis.
34544272	4	56	theme	probes	663:668	arg1	Use					628:630	Use	628:630	Use of these fluorescent cell wall probes	628:668	Use of these fluorescent cell wall probes and peptidoglycan compositional analysis demonstrate that a single class A penicillin-binding protein is essential for polar peptidoglycan synthesis.
34544272	2	57	theme	cell	324:327	arg1	enzymes					344:350	cell wall synthesis enzymes	324:350	cell wall synthesis enzymes	324:350	To investigate the mechanisms underlying polar cell wall synthesis, we systematically probed the function of cell wall synthesis enzymes in the plant pathogen Agrobacterium tumefaciens.
34544272	9	58	theme	lateral	1533:1539	arg1	elongation					1541:1550	lateral elongation	1533:1550	lateral elongation	1533:1550	It is increasingly clear that rod-shaped bacteria use a diverse array of growth strategies with distinct spatial zones of cell wall biosynthesis, including lateral elongation, unipolar growth, bipolar elongation, and medial elongation.
34544272	8	59	theme	cell	1261:1264	arg1	wall					1266:1269	the bacterial cell wall	1247:1269	the bacterial cell wall	1247:1269	IMPORTANCE While the structure and function of the bacterial cell wall are well conserved, the mechanisms responsible for cell wall biosynthesis during elongation are variable.
34544272	6	60	theme	growth	1028:1033	arg1	mechanism					1006:1014	the mechanism	1002:1014	the mechanism of unipolar growth	1002:1033	Genetic analysis and cell wall targeting antibiotics reveal that the mechanism of unipolar growth is conserved in Sinorhizobium and Brucella.
34544272	8	61	theme	cell	1322:1325	arg1	biosynthesis					1332:1343	cell wall biosynthesis	1322:1343	cell wall biosynthesis during elongation	1322:1361	IMPORTANCE While the structure and function of the bacterial cell wall are well conserved, the mechanisms responsible for cell wall biosynthesis during elongation are variable.
34544272	5	62	theme	mode	867:870	arg1	evidence					841:848	evidence	841:848	evidence of an additional mode of cell wall synthesis that requires ld-transpeptidase activity	841:934	Furthermore, we find evidence of an additional mode of cell wall synthesis that requires ld-transpeptidase activity.
34544272	10	63	theme	lateral	1730:1736	arg1	elongation					1738:1747	lateral elongation	1730:1747	lateral elongation	1730:1747	Yet the vast majority of our understanding regarding bacterial elongation is derived from model organisms exhibiting lateral elongation.
34544272	7	64	theme	unipolar	1112:1119	arg1	biosynthesis					1135:1146	unipolar peptidoglycan biosynthesis	1112:1146	unipolar peptidoglycan biosynthesis employed by the Rhizobiales during cell elongation	1112:1197	This work provides insights into unipolar peptidoglycan biosynthesis employed by the Rhizobiales during cell elongation.
34544272	4	65	theme	peptidoglycan	674:686	arg1	analysis					702:709	peptidoglycan compositional analysis	674:709	peptidoglycan compositional analysis	674:709	Use of these fluorescent cell wall probes and peptidoglycan compositional analysis demonstrate that a single class A penicillin-binding protein is essential for polar peptidoglycan synthesis.
34544272	5	66	theme	cell	875:878	arg1	synthesis					885:893	cell wall synthesis	875:893	cell wall synthesis	875:893	Furthermore, we find evidence of an additional mode of cell wall synthesis that requires ld-transpeptidase activity.
34544272	4	67	theme	cell	653:656	arg1	probes					663:668	these fluorescent cell wall probes	635:668	these fluorescent cell wall probes	635:668	Use of these fluorescent cell wall probes and peptidoglycan compositional analysis demonstrate that a single class A penicillin-binding protein is essential for polar peptidoglycan synthesis.
34544272	3	68	theme	growth	596:601	arg1	patterns					603:610	growth patterns	596:610	growth patterns	596:610	The development of fluorescent d-amino acid dipeptide (FDAAD) probes, which are incorporated into peptidoglycan by penicillin-binding proteins in A. tumefaciens, enabled us to monitor changes in growth patterns in the mutants.
34544272	9	69	theme	bipolar	1570:1576	arg1	elongation					1578:1587	bipolar elongation	1570:1587	bipolar elongation	1570:1587	It is increasingly clear that rod-shaped bacteria use a diverse array of growth strategies with distinct spatial zones of cell wall biosynthesis, including lateral elongation, unipolar growth, bipolar elongation, and medial elongation.
34544272	4	70	theme	single	730:735	arg1	essential					775:783	essential	775:783	essential	775:783	Use of these fluorescent cell wall probes and peptidoglycan compositional analysis demonstrate that a single class A penicillin-binding protein is essential for polar peptidoglycan synthesis.
34544272	4	70	theme	single	730:735	arg1	class					737:741	a single class	728:741	a single class A penicillin-binding protein	728:770	Use of these fluorescent cell wall probes and peptidoglycan compositional analysis demonstrate that a single class A penicillin-binding protein is essential for polar peptidoglycan synthesis.
34544272	10	71	attach	derived	1690:1696	arg1	organisms					1709:1717	model organisms	1703:1717	model organisms exhibiting lateral elongation	1703:1747	Yet the vast majority of our understanding regarding bacterial elongation is derived from model organisms exhibiting lateral elongation.
34544272	10	71	attach	derived	1690:1696	arg2	majority					1626:1633	the vast majority	1617:1633	the vast majority of our understanding regarding bacterial elongation	1617:1685	Yet the vast majority of our understanding regarding bacterial elongation is derived from model organisms exhibiting lateral elongation.
34544272	5	72	theme	ld-transpeptidase	909:925	arg1	activity					927:934	ld-transpeptidase activity	909:934	ld-transpeptidase activity	909:934	Furthermore, we find evidence of an additional mode of cell wall synthesis that requires ld-transpeptidase activity.
34544272	3	73	from	changes	585:591	arg1	patterns					603:610	growth patterns	596:610	growth patterns	596:610	The development of fluorescent d-amino acid dipeptide (FDAAD) probes, which are incorporated into peptidoglycan by penicillin-binding proteins in A. tumefaciens, enabled us to monitor changes in growth patterns in the mutants.
34544272	3	73	from	changes	585:591	arg1	mutants					619:625	the mutants	615:625	the mutants	615:625	The development of fluorescent d-amino acid dipeptide (FDAAD) probes, which are incorporated into peptidoglycan by penicillin-binding proteins in A. tumefaciens, enabled us to monitor changes in growth patterns in the mutants.
34544272	11	74	theme	bacteria	1871:1878	arg1	elongation					1819:1828	unipolar elongation	1810:1828	unipolar elongation of Agrobacterium tumefaciens and related bacteria	1810:1878	Here, we explore the role of penicillin-binding proteins in unipolar elongation of Agrobacterium tumefaciens and related bacteria within the Rhizobiales.
34544272	0	75	theme	Class	74:78	arg1	Protein					101:107	an Essential Class A Penicillin-Binding Protein	61:107	an Essential Class A Penicillin-Binding Protein	61:107	Unipolar Peptidoglycan Synthesis in the Rhizobiales Requires an Essential Class A Penicillin-Binding Protein.
34544272	2	76	theme	cell	262:265	arg1	synthesis					272:280	polar cell wall synthesis	256:280	polar cell wall synthesis	256:280	To investigate the mechanisms underlying polar cell wall synthesis, we systematically probed the function of cell wall synthesis enzymes in the plant pathogen Agrobacterium tumefaciens.
34544272	1	77	theme	Rod	202:204	arg1	complex					206:212	the canonical Rod complex	188:212	the canonical Rod complex	188:212	Members of the Rhizobiales are polarly growing bacteria that lack homologs of the canonical Rod complex.
34544272	9	78	theme	medial	1594:1599	arg1	elongation					1601:1610	medial elongation	1594:1610	medial elongation	1594:1610	It is increasingly clear that rod-shaped bacteria use a diverse array of growth strategies with distinct spatial zones of cell wall biosynthesis, including lateral elongation, unipolar growth, bipolar elongation, and medial elongation.
34544272	0	79	theme	Penicillin-Binding	82:99	arg1	Protein					101:107	an Essential Class A Penicillin-Binding Protein	61:107	an Essential Class A Penicillin-Binding Protein	61:107	Unipolar Peptidoglycan Synthesis in the Rhizobiales Requires an Essential Class A Penicillin-Binding Protein.
34544272	4	80	theme	polar	789:793	arg1	synthesis					809:817	polar peptidoglycan synthesis	789:817	polar peptidoglycan synthesis	789:817	Use of these fluorescent cell wall probes and peptidoglycan compositional analysis demonstrate that a single class A penicillin-binding protein is essential for polar peptidoglycan synthesis.
34544272	1	81	theme	complex	206:212	arg1	homologs					176:183	homologs	176:183	homologs of the canonical Rod complex	176:212	Members of the Rhizobiales are polarly growing bacteria that lack homologs of the canonical Rod complex.
34544272	8	82	dep	structure	1221:1229	arg1	the					1217:1219	the	1217:1219	the	1217:1219	IMPORTANCE While the structure and function of the bacterial cell wall are well conserved, the mechanisms responsible for cell wall biosynthesis during elongation are variable.
34544272	0	83	theme	Peptidoglycan	9:21	arg1	Synthesis					23:31	Unipolar Peptidoglycan Synthesis	0:31	Unipolar Peptidoglycan Synthesis in the Rhizobiales	0:50	Unipolar Peptidoglycan Synthesis in the Rhizobiales Requires an Essential Class A Penicillin-Binding Protein.
34544272	9	84	theme	rod-shaped	1407:1416	arg1	bacteria					1418:1425	rod-shaped bacteria	1407:1425	rod-shaped bacteria	1407:1425	It is increasingly clear that rod-shaped bacteria use a diverse array of growth strategies with distinct spatial zones of cell wall biosynthesis, including lateral elongation, unipolar growth, bipolar elongation, and medial elongation.
34544272	12	85	theme	ld-transpeptidases	1984:2001	arg1	subset					1974:1979	a subset	1972:1979	a subset of ld-transpeptidases	1972:2001	Our findings suggest that penicillin-binding protein 1a, along with a subset of ld-transpeptidases, drives unipolar growth.
34544272	4	86	dep	class	737:741	arg1	protein					764:770	A penicillin-binding protein	743:770	a single class A penicillin-binding protein	728:770	Use of these fluorescent cell wall probes and peptidoglycan compositional analysis demonstrate that a single class A penicillin-binding protein is essential for polar peptidoglycan synthesis.
34544272	11	87	from	role	1771:1774	arg1	elongation					1819:1828	unipolar elongation	1810:1828	unipolar elongation of Agrobacterium tumefaciens and related bacteria	1810:1878	Here, we explore the role of penicillin-binding proteins in unipolar elongation of Agrobacterium tumefaciens and related bacteria within the Rhizobiales.
34544272	11	88	theme	proteins	1798:1805	arg1	role					1771:1774	the role	1767:1774	the role of penicillin-binding proteins in unipolar elongation of Agrobacterium tumefaciens and related bacteria	1767:1878	Here, we explore the role of penicillin-binding proteins in unipolar elongation of Agrobacterium tumefaciens and related bacteria within the Rhizobiales.
34544272	9	89	theme	diverse	1433:1439	arg1	array					1441:1445	a diverse array	1431:1445	a diverse array of growth strategies	1431:1466	It is increasingly clear that rod-shaped bacteria use a diverse array of growth strategies with distinct spatial zones of cell wall biosynthesis, including lateral elongation, unipolar growth, bipolar elongation, and medial elongation.
34544272	3	90	theme	acid	440:443	arg1	probes					463:468	fluorescent d-amino acid dipeptide (FDAAD) probes	420:468	fluorescent d-amino acid dipeptide (FDAAD) probes	420:468	The development of fluorescent d-amino acid dipeptide (FDAAD) probes, which are incorporated into peptidoglycan by penicillin-binding proteins in A. tumefaciens, enabled us to monitor changes in growth patterns in the mutants.
34544272	12	91	theme	penicillin-binding	1930:1947	arg1	1a					1957:1958	penicillin-binding protein 1a	1930:1958	penicillin-binding protein 1a	1930:1958	Our findings suggest that penicillin-binding protein 1a, along with a subset of ld-transpeptidases, drives unipolar growth.
34544272	3	92	theme	fluorescent	420:430	arg1	probes					463:468	fluorescent d-amino acid dipeptide (FDAAD) probes	420:468	fluorescent d-amino acid dipeptide (FDAAD) probes	420:468	The development of fluorescent d-amino acid dipeptide (FDAAD) probes, which are incorporated into peptidoglycan by penicillin-binding proteins in A. tumefaciens, enabled us to monitor changes in growth patterns in the mutants.
34544272	2	93	theme	enzymes	344:350	arg1	function					312:319	the function	308:319	the function of cell wall synthesis enzymes in the plant pathogen Agrobacterium tumefaciens	308:398	To investigate the mechanisms underlying polar cell wall synthesis, we systematically probed the function of cell wall synthesis enzymes in the plant pathogen Agrobacterium tumefaciens.
34544272	9	94	theme	strategies	1457:1466	arg1	array					1441:1445	a diverse array	1431:1445	a diverse array of growth strategies	1431:1466	It is increasingly clear that rod-shaped bacteria use a diverse array of growth strategies with distinct spatial zones of cell wall biosynthesis, including lateral elongation, unipolar growth, bipolar elongation, and medial elongation.
34544272	11	95	theme	unipolar	1810:1817	arg1	elongation					1819:1828	unipolar elongation	1810:1828	unipolar elongation of Agrobacterium tumefaciens and related bacteria	1810:1878	Here, we explore the role of penicillin-binding proteins in unipolar elongation of Agrobacterium tumefaciens and related bacteria within the Rhizobiales.
34544272	13	96	theme	Rhizobiales	2109:2119	arg1	biocontrol					2084:2093	biocontrol	2084:2093	biocontrol of pathogenic Rhizobiales	2084:2119	Thus, these enzymes may serve as attractive targets for biocontrol of pathogenic Rhizobiales.
34544272	2	97	theme	wall	329:332	arg1	enzymes					344:350	cell wall synthesis enzymes	324:350	cell wall synthesis enzymes	324:350	To investigate the mechanisms underlying polar cell wall synthesis, we systematically probed the function of cell wall synthesis enzymes in the plant pathogen Agrobacterium tumefaciens.
34544272	9	98	theme	distinct	1473:1480	arg1	elongation					1601:1610	medial elongation	1594:1610	medial elongation	1594:1610	It is increasingly clear that rod-shaped bacteria use a diverse array of growth strategies with distinct spatial zones of cell wall biosynthesis, including lateral elongation, unipolar growth, bipolar elongation, and medial elongation.
34544272	9	98	theme	distinct	1473:1480	arg1	elongation					1541:1550	lateral elongation	1533:1550	lateral elongation	1533:1550	It is increasingly clear that rod-shaped bacteria use a diverse array of growth strategies with distinct spatial zones of cell wall biosynthesis, including lateral elongation, unipolar growth, bipolar elongation, and medial elongation.
34544272	9	98	theme	distinct	1473:1480	arg1	zones					1490:1494	distinct spatial zones	1473:1494	distinct spatial zones	1473:1494	It is increasingly clear that rod-shaped bacteria use a diverse array of growth strategies with distinct spatial zones of cell wall biosynthesis, including lateral elongation, unipolar growth, bipolar elongation, and medial elongation.
34544272	9	98	theme	distinct	1473:1480	arg1	growth					1562:1567	unipolar growth	1553:1567	unipolar growth	1553:1567	It is increasingly clear that rod-shaped bacteria use a diverse array of growth strategies with distinct spatial zones of cell wall biosynthesis, including lateral elongation, unipolar growth, bipolar elongation, and medial elongation.
34544272	9	98	theme	distinct	1473:1480	arg1	elongation					1578:1587	bipolar elongation	1570:1587	bipolar elongation	1570:1587	It is increasingly clear that rod-shaped bacteria use a diverse array of growth strategies with distinct spatial zones of cell wall biosynthesis, including lateral elongation, unipolar growth, bipolar elongation, and medial elongation.
34544272	2	99	theme	pathogen	365:372	arg1	tumefaciens					388:398	the plant pathogen Agrobacterium tumefaciens	355:398	the plant pathogen Agrobacterium tumefaciens	355:398	To investigate the mechanisms underlying polar cell wall synthesis, we systematically probed the function of cell wall synthesis enzymes in the plant pathogen Agrobacterium tumefaciens.
32409073	2	0	theme	hydrogels	498:506	arg1	properties					472:481	the physical and mechanical properties	444:481	the physical and mechanical properties of as-prepared hydrogels	444:506	Linear and star-shaped poly(l-lysine) (l-PLL and s-PLL) were chosen due to not only their cationic characteristics, facilitating the efficient complexation of negatively charged heparin, but also the ease to tune the physical and mechanical properties of as-prepared hydrogels simply by varying polypeptide topology and chain length.
32409073	1	1	theme	composite	180:188	arg1	hydrogels					190:198	polypeptide/heparin composite hydrogels	160:198	polypeptide/heparin composite hydrogels for wound healing application	160:228	We report an efficient growth factor delivering system based on polypeptide/heparin composite hydrogels for wound healing application.
32409073	2	2	theme	as-prepared	486:496	arg1	hydrogels					498:506	as-prepared hydrogels	486:506	as-prepared hydrogels	486:506	Linear and star-shaped poly(l-lysine) (l-PLL and s-PLL) were chosen due to not only their cationic characteristics, facilitating the efficient complexation of negatively charged heparin, but also the ease to tune the physical and mechanical properties of as-prepared hydrogels simply by varying polypeptide topology and chain length.
32409073	6	3	theme	biomedical	1277:1286	arg1	fields					1288:1293	other biomedical fields	1271:1293	other biomedical fields	1271:1293	We believe that these PLL/heparin composite hydrogels are promising wound dressing materials and may have potential applications in other biomedical fields.
32409073	6	4	theme	wound	1207:1211	arg1	materials					1222:1230	promising wound dressing materials	1197:1230	promising wound dressing materials	1197:1230	We believe that these PLL/heparin composite hydrogels are promising wound dressing materials and may have potential applications in other biomedical fields.
32409073	6	4	theme	wound	1207:1211	arg1	hydrogels					1183:1191	these PLL/heparin composite hydrogels	1155:1191	these PLL/heparin composite hydrogels	1155:1191	We believe that these PLL/heparin composite hydrogels are promising wound dressing materials and may have potential applications in other biomedical fields.
32409073	6	5	theme	promising	1197:1205	arg1	materials					1222:1230	promising wound dressing materials	1197:1230	promising wound dressing materials	1197:1230	We believe that these PLL/heparin composite hydrogels are promising wound dressing materials and may have potential applications in other biomedical fields.
32409073	6	5	theme	promising	1197:1205	arg1	hydrogels					1183:1191	these PLL/heparin composite hydrogels	1155:1191	these PLL/heparin composite hydrogels	1155:1191	We believe that these PLL/heparin composite hydrogels are promising wound dressing materials and may have potential applications in other biomedical fields.
32409073	5	6	with	hydrogels	933:941	arg1	heparin					948:954	heparin	948:954	heparin	948:954	Unlike other heparin-based hydrogels, these composite hydrogels with heparin densely deposited on the surface can increase the stabilization and concentration of growth factor, which can facilitate the healing process as confirmed by our in vivo animal model.
32409073	2	7	theme	efficient	364:372	arg1	complexation					374:385	the efficient complexation	360:385	the efficient complexation of negatively charged heparin	360:415	Linear and star-shaped poly(l-lysine) (l-PLL and s-PLL) were chosen due to not only their cationic characteristics, facilitating the efficient complexation of negatively charged heparin, but also the ease to tune the physical and mechanical properties of as-prepared hydrogels simply by varying polypeptide topology and chain length.
32409073	2	8	theme	polypeptide	526:536	arg1	topology					538:545	polypeptide topology	526:545	polypeptide topology	526:545	Linear and star-shaped poly(l-lysine) (l-PLL and s-PLL) were chosen due to not only their cationic characteristics, facilitating the efficient complexation of negatively charged heparin, but also the ease to tune the physical and mechanical properties of as-prepared hydrogels simply by varying polypeptide topology and chain length.
32409073	6	9	theme	composite	1173:1181	arg1	materials					1222:1230	promising wound dressing materials	1197:1230	promising wound dressing materials	1197:1230	We believe that these PLL/heparin composite hydrogels are promising wound dressing materials and may have potential applications in other biomedical fields.
32409073	6	9	theme	composite	1173:1181	arg1	hydrogels					1183:1191	these PLL/heparin composite hydrogels	1155:1191	these PLL/heparin composite hydrogels	1155:1191	We believe that these PLL/heparin composite hydrogels are promising wound dressing materials and may have potential applications in other biomedical fields.
32409073	4	10	theme	antibacterial	808:820	arg1	activity					822:829	excellent antibacterial activity	798:829	excellent antibacterial activity	798:829	Our experimental data showed that these composite hydrogels exhibited low hemolytic activity and good cell compatibility as well as excellent antibacterial activity, making them ideal as wound dressing materials.
32409073	1	11	theme	healing	210:216	arg1	application					218:228	wound healing application	204:228	wound healing application	204:228	We report an efficient growth factor delivering system based on polypeptide/heparin composite hydrogels for wound healing application.
32409073	5	12	theme	heparin-based	892:904	arg1	hydrogels					906:914	other heparin-based hydrogels	886:914	other heparin-based hydrogels	886:914	Unlike other heparin-based hydrogels, these composite hydrogels with heparin densely deposited on the surface can increase the stabilization and concentration of growth factor, which can facilitate the healing process as confirmed by our in vivo animal model.
32409073	4	13	theme	excellent	798:806	arg1	activity					822:829	excellent antibacterial activity	798:829	excellent antibacterial activity	798:829	Our experimental data showed that these composite hydrogels exhibited low hemolytic activity and good cell compatibility as well as excellent antibacterial activity, making them ideal as wound dressing materials.
32409073	1	14	theme	wound	204:208	arg1	healing					210:216	wound healing	204:216	wound healing application	204:228	We report an efficient growth factor delivering system based on polypeptide/heparin composite hydrogels for wound healing application.
32409073	2	15	theme	physical	448:455	arg1	properties					472:481	the physical and mechanical properties	444:481	the physical and mechanical properties of as-prepared hydrogels	444:506	Linear and star-shaped poly(l-lysine) (l-PLL and s-PLL) were chosen due to not only their cationic characteristics, facilitating the efficient complexation of negatively charged heparin, but also the ease to tune the physical and mechanical properties of as-prepared hydrogels simply by varying polypeptide topology and chain length.
32409073	4	16	theme	experimental	670:681	arg1	data					683:686	Our experimental data	666:686	Our experimental data	666:686	Our experimental data showed that these composite hydrogels exhibited low hemolytic activity and good cell compatibility as well as excellent antibacterial activity, making them ideal as wound dressing materials.
32409073	4	17	theme	cell	768:771	arg1	compatibility					773:785	good cell compatibility	763:785	good cell compatibility	763:785	Our experimental data showed that these composite hydrogels exhibited low hemolytic activity and good cell compatibility as well as excellent antibacterial activity, making them ideal as wound dressing materials.
32409073	2	18	theme	charged	401:407	arg1	heparin					409:415	negatively charged heparin	390:415	negatively charged heparin	390:415	Linear and star-shaped poly(l-lysine) (l-PLL and s-PLL) were chosen due to not only their cationic characteristics, facilitating the efficient complexation of negatively charged heparin, but also the ease to tune the physical and mechanical properties of as-prepared hydrogels simply by varying polypeptide topology and chain length.
32409073	0	19	theme	Antibacterial	0:12	arg1	hydrogels					44:52	Antibacterial polypeptide/heparin composite hydrogels	0:52	Antibacterial polypeptide/heparin composite hydrogels	0:52	Antibacterial polypeptide/heparin composite hydrogels carrying growth factor for wound healing.
32409073	6	20	theme	potential	1245:1253	arg1	applications					1255:1266	potential applications	1245:1266	potential applications	1245:1266	We believe that these PLL/heparin composite hydrogels are promising wound dressing materials and may have potential applications in other biomedical fields.
32409073	3	21	theme	polymer	589:595	arg1	parameter					627:635	an additional parameter	613:635	an additional parameter to tune hydrogel properties	613:663	The results showed that polymer topology can be an additional parameter to tune hydrogel properties.
32409073	3	21	theme	polymer	589:595	arg1	topology					597:604	polymer topology	589:604	polymer topology	589:604	The results showed that polymer topology can be an additional parameter to tune hydrogel properties.
32409073	4	22	theme	good	763:766	arg1	compatibility					773:785	good cell compatibility	763:785	good cell compatibility	763:785	Our experimental data showed that these composite hydrogels exhibited low hemolytic activity and good cell compatibility as well as excellent antibacterial activity, making them ideal as wound dressing materials.
32409073	4	23	theme	wound	853:857	arg1	materials					868:876	wound dressing materials	853:876	wound dressing materials	853:876	Our experimental data showed that these composite hydrogels exhibited low hemolytic activity and good cell compatibility as well as excellent antibacterial activity, making them ideal as wound dressing materials.
32409073	1	24	theme	efficient	109:117	arg1	factor					126:131	an efficient growth factor	106:131	an efficient growth factor delivering system based on polypeptide/heparin composite hydrogels for wound healing application	106:228	We report an efficient growth factor delivering system based on polypeptide/heparin composite hydrogels for wound healing application.
32409073	0	25	theme	composite	34:42	arg1	hydrogels					44:52	Antibacterial polypeptide/heparin composite hydrogels	0:52	Antibacterial polypeptide/heparin composite hydrogels	0:52	Antibacterial polypeptide/heparin composite hydrogels carrying growth factor for wound healing.
32409073	1	26	theme	growth	119:124	arg1	factor					126:131	an efficient growth factor	106:131	an efficient growth factor delivering system based on polypeptide/heparin composite hydrogels for wound healing application	106:228	We report an efficient growth factor delivering system based on polypeptide/heparin composite hydrogels for wound healing application.
32409073	2	27	dep	poly	254:257	arg1	l-PLL					270:274	l-PLL	270:274	l-PLL	270:274	Linear and star-shaped poly(l-lysine) (l-PLL and s-PLL) were chosen due to not only their cationic characteristics, facilitating the efficient complexation of negatively charged heparin, but also the ease to tune the physical and mechanical properties of as-prepared hydrogels simply by varying polypeptide topology and chain length.
32409073	2	27	dep	poly	254:257	arg1	s-PLL					280:284	s-PLL	280:284	s-PLL	280:284	Linear and star-shaped poly(l-lysine) (l-PLL and s-PLL) were chosen due to not only their cationic characteristics, facilitating the efficient complexation of negatively charged heparin, but also the ease to tune the physical and mechanical properties of as-prepared hydrogels simply by varying polypeptide topology and chain length.
32409073	0	28	theme	growth	63:68	arg1	factor					70:75	growth factor	63:75	growth factor for wound healing	63:93	Antibacterial polypeptide/heparin composite hydrogels carrying growth factor for wound healing.
32409073	6	29	theme	PLL/heparin	1161:1171	arg1	materials					1222:1230	promising wound dressing materials	1197:1230	promising wound dressing materials	1197:1230	We believe that these PLL/heparin composite hydrogels are promising wound dressing materials and may have potential applications in other biomedical fields.
32409073	6	29	theme	PLL/heparin	1161:1171	arg1	hydrogels					1183:1191	these PLL/heparin composite hydrogels	1155:1191	these PLL/heparin composite hydrogels	1155:1191	We believe that these PLL/heparin composite hydrogels are promising wound dressing materials and may have potential applications in other biomedical fields.
32409073	5	30	theme	in	1117:1118	arg1	model					1132:1136	our in vivo animal model	1113:1136	our in vivo animal model	1113:1136	Unlike other heparin-based hydrogels, these composite hydrogels with heparin densely deposited on the surface can increase the stabilization and concentration of growth factor, which can facilitate the healing process as confirmed by our in vivo animal model.
32409073	6	31	contain	have	1240:1243	arg1	materials					1222:1230	promising wound dressing materials	1197:1230	promising wound dressing materials	1197:1230	We believe that these PLL/heparin composite hydrogels are promising wound dressing materials and may have potential applications in other biomedical fields.
32409073	6	31	contain	have	1240:1243	arg1	hydrogels					1183:1191	these PLL/heparin composite hydrogels	1155:1191	these PLL/heparin composite hydrogels	1155:1191	We believe that these PLL/heparin composite hydrogels are promising wound dressing materials and may have potential applications in other biomedical fields.
32409073	6	31	contain	have	1240:1243	arg2	applications					1255:1266	potential applications	1245:1266	potential applications	1245:1266	We believe that these PLL/heparin composite hydrogels are promising wound dressing materials and may have potential applications in other biomedical fields.
32409073	2	32	theme	chain	551:555	arg1	length					557:562	chain length	551:562	chain length	551:562	Linear and star-shaped poly(l-lysine) (l-PLL and s-PLL) were chosen due to not only their cationic characteristics, facilitating the efficient complexation of negatively charged heparin, but also the ease to tune the physical and mechanical properties of as-prepared hydrogels simply by varying polypeptide topology and chain length.
32409073	3	33	theme	hydrogel	645:652	arg1	properties					654:663	hydrogel properties	645:663	hydrogel properties	645:663	The results showed that polymer topology can be an additional parameter to tune hydrogel properties.
32409073	5	34	theme	other	886:890	arg1	hydrogels					906:914	other heparin-based hydrogels	886:914	other heparin-based hydrogels	886:914	Unlike other heparin-based hydrogels, these composite hydrogels with heparin densely deposited on the surface can increase the stabilization and concentration of growth factor, which can facilitate the healing process as confirmed by our in vivo animal model.
32409073	2	35	theme	mechanical	461:470	arg1	properties					472:481	the physical and mechanical properties	444:481	the physical and mechanical properties of as-prepared hydrogels	444:506	Linear and star-shaped poly(l-lysine) (l-PLL and s-PLL) were chosen due to not only their cationic characteristics, facilitating the efficient complexation of negatively charged heparin, but also the ease to tune the physical and mechanical properties of as-prepared hydrogels simply by varying polypeptide topology and chain length.
32409073	5	36	theme	composite	923:931	arg1	hydrogels					933:941	these composite hydrogels	917:941	these composite hydrogels with heparin densely deposited on the surface	917:987	Unlike other heparin-based hydrogels, these composite hydrogels with heparin densely deposited on the surface can increase the stabilization and concentration of growth factor, which can facilitate the healing process as confirmed by our in vivo animal model.
32409073	5	37	theme	animal	1125:1130	arg1	model					1132:1136	our in vivo animal model	1113:1136	our in vivo animal model	1113:1136	Unlike other heparin-based hydrogels, these composite hydrogels with heparin densely deposited on the surface can increase the stabilization and concentration of growth factor, which can facilitate the healing process as confirmed by our in vivo animal model.
32409073	4	38	theme	dressing	859:866	arg1	materials					868:876	wound dressing materials	853:876	wound dressing materials	853:876	Our experimental data showed that these composite hydrogels exhibited low hemolytic activity and good cell compatibility as well as excellent antibacterial activity, making them ideal as wound dressing materials.
32409073	4	39	theme	hemolytic	740:748	arg1	activity					750:757	low hemolytic activity	736:757	low hemolytic activity	736:757	Our experimental data showed that these composite hydrogels exhibited low hemolytic activity and good cell compatibility as well as excellent antibacterial activity, making them ideal as wound dressing materials.
32409073	5	40	theme	healing	1081:1087	arg1	process					1089:1095	the healing process	1077:1095	the healing process	1077:1095	Unlike other heparin-based hydrogels, these composite hydrogels with heparin densely deposited on the surface can increase the stabilization and concentration of growth factor, which can facilitate the healing process as confirmed by our in vivo animal model.
32409073	1	41	theme	polypeptide/heparin	160:178	arg1	hydrogels					190:198	polypeptide/heparin composite hydrogels	160:198	polypeptide/heparin composite hydrogels for wound healing application	160:228	We report an efficient growth factor delivering system based on polypeptide/heparin composite hydrogels for wound healing application.
32409073	4	42	theme	low	736:738	arg1	activity					750:757	low hemolytic activity	736:757	low hemolytic activity	736:757	Our experimental data showed that these composite hydrogels exhibited low hemolytic activity and good cell compatibility as well as excellent antibacterial activity, making them ideal as wound dressing materials.
32409073	6	43	theme	other	1271:1275	arg1	fields					1288:1293	other biomedical fields	1271:1293	other biomedical fields	1271:1293	We believe that these PLL/heparin composite hydrogels are promising wound dressing materials and may have potential applications in other biomedical fields.
32409073	5	44	theme	growth	1041:1046	arg1	factor					1048:1053	growth factor	1041:1053	growth factor	1041:1053	Unlike other heparin-based hydrogels, these composite hydrogels with heparin densely deposited on the surface can increase the stabilization and concentration of growth factor, which can facilitate the healing process as confirmed by our in vivo animal model.
32409073	5	45	dep	stabilization	1006:1018	arg1	the					1002:1004	the	1002:1004	the	1002:1004	Unlike other heparin-based hydrogels, these composite hydrogels with heparin densely deposited on the surface can increase the stabilization and concentration of growth factor, which can facilitate the healing process as confirmed by our in vivo animal model.
32409073	6	46	theme	dressing	1213:1220	arg1	materials					1222:1230	promising wound dressing materials	1197:1230	promising wound dressing materials	1197:1230	We believe that these PLL/heparin composite hydrogels are promising wound dressing materials and may have potential applications in other biomedical fields.
32409073	6	46	theme	dressing	1213:1220	arg1	hydrogels					1183:1191	these PLL/heparin composite hydrogels	1155:1191	these PLL/heparin composite hydrogels	1155:1191	We believe that these PLL/heparin composite hydrogels are promising wound dressing materials and may have potential applications in other biomedical fields.
32409073	5	47	theme	factor	1048:1053	arg1	concentration					1024:1036	concentration	1024:1036	concentration	1024:1036	Unlike other heparin-based hydrogels, these composite hydrogels with heparin densely deposited on the surface can increase the stabilization and concentration of growth factor, which can facilitate the healing process as confirmed by our in vivo animal model.
32409073	5	47	theme	factor	1048:1053	arg1	stabilization					1006:1018	stabilization	1006:1018	stabilization	1006:1018	Unlike other heparin-based hydrogels, these composite hydrogels with heparin densely deposited on the surface can increase the stabilization and concentration of growth factor, which can facilitate the healing process as confirmed by our in vivo animal model.
32409073	2	48	theme	cationic	321:328	arg1	characteristics					330:344	their cationic characteristics	315:344	their cationic characteristics	315:344	Linear and star-shaped poly(l-lysine) (l-PLL and s-PLL) were chosen due to not only their cationic characteristics, facilitating the efficient complexation of negatively charged heparin, but also the ease to tune the physical and mechanical properties of as-prepared hydrogels simply by varying polypeptide topology and chain length.
32409073	3	49	theme	additional	616:625	arg1	parameter					627:635	an additional parameter	613:635	an additional parameter to tune hydrogel properties	613:663	The results showed that polymer topology can be an additional parameter to tune hydrogel properties.
32409073	3	49	theme	additional	616:625	arg1	topology					597:604	polymer topology	589:604	polymer topology	589:604	The results showed that polymer topology can be an additional parameter to tune hydrogel properties.
32409073	4	50	theme	composite	706:714	arg1	hydrogels					716:724	these composite hydrogels	700:724	these composite hydrogels	700:724	Our experimental data showed that these composite hydrogels exhibited low hemolytic activity and good cell compatibility as well as excellent antibacterial activity, making them ideal as wound dressing materials.
32409073	2	51	theme	heparin	409:415	arg1	complexation					374:385	the efficient complexation	360:385	the efficient complexation of negatively charged heparin	360:415	Linear and star-shaped poly(l-lysine) (l-PLL and s-PLL) were chosen due to not only their cationic characteristics, facilitating the efficient complexation of negatively charged heparin, but also the ease to tune the physical and mechanical properties of as-prepared hydrogels simply by varying polypeptide topology and chain length.
32409073	2	52	dep	star-shaped	242:252	arg1	l-lysine					259:266	l-lysine	259:266	l-lysine	259:266	Linear and star-shaped poly(l-lysine) (l-PLL and s-PLL) were chosen due to not only their cationic characteristics, facilitating the efficient complexation of negatively charged heparin, but also the ease to tune the physical and mechanical properties of as-prepared hydrogels simply by varying polypeptide topology and chain length.
32409073	2	52	dep	star-shaped	242:252	arg1	poly					254:257	poly	254:257	poly(l-lysine) (l-PLL and s-PLL)	254:285	Linear and star-shaped poly(l-lysine) (l-PLL and s-PLL) were chosen due to not only their cationic characteristics, facilitating the efficient complexation of negatively charged heparin, but also the ease to tune the physical and mechanical properties of as-prepared hydrogels simply by varying polypeptide topology and chain length.
32409073	0	53	theme	wound	81:85	arg1	healing					87:93	wound healing	81:93	wound healing	81:93	Antibacterial polypeptide/heparin composite hydrogels carrying growth factor for wound healing.
32409073	0	54	theme	polypeptide/heparin	14:32	arg1	hydrogels					44:52	Antibacterial polypeptide/heparin composite hydrogels	0:52	Antibacterial polypeptide/heparin composite hydrogels	0:52	Antibacterial polypeptide/heparin composite hydrogels carrying growth factor for wound healing.
32409073	5	55	dep	in	1117:1118	arg1	vivo					1120:1123	vivo	1120:1123	vivo	1120:1123	Unlike other heparin-based hydrogels, these composite hydrogels with heparin densely deposited on the surface can increase the stabilization and concentration of growth factor, which can facilitate the healing process as confirmed by our in vivo animal model.
34453980	4	0	theme	thermal	993:999	arg1	stability					1001:1009	thermal stability	993:1009	thermal stability of film	993:1017	In addition, incorporating PAL@ZnO nanorods also significantly enhanced the water resistance, and thermal stability of film.
34453980	3	1	theme	films	757:761	arg1	toughness					744:752	The toughness	740:752	The toughness of films	740:761	The toughness of films enhances to 35.13 ± 0.95 MPa and the moisture uptake decreases to 23.74 ± 0.02% after embedding 3% and 9% of PAL@ZnO, respectively.
34453980	0	2	theme	antibacterial	89:101	arg1	properties					103:112	antibacterial properties	89:112	antibacterial properties of chitosan-based film	89:135	Multifunctional palygorskite@ZnO nanorods enhance simultaneously mechanical strength and antibacterial properties of chitosan-based film.
34453980	2	3	theme	PAL	400:402	arg1	nanorod					405:411	non-toxic natural palygorskite (PAL) nanorod	368:411	non-toxic natural palygorskite (PAL) nanorod	368:411	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	0	4	theme	chitosan-based	117:130	arg1	film					132:135	chitosan-based film	117:135	chitosan-based film	117:135	Multifunctional palygorskite@ZnO nanorods enhance simultaneously mechanical strength and antibacterial properties of chitosan-based film.
34453980	5	5	theme	films	1095:1099	arg1	development					1066:1076	the development	1062:1076	the development of antibacterial films with potential applications in many fields such as food packing	1062:1163	This work provides an alternative way for the development of antibacterial films with potential applications in many fields such as food packing.
34453980	2	6	theme	composite	537:545	arg1	films					547:551	the composite films	533:551	the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively)	533:737	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	2	7	theme	non-toxic	368:376	arg1	nanorod					405:411	non-toxic natural palygorskite (PAL) nanorod	368:411	non-toxic natural palygorskite (PAL) nanorod	368:411	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	4	8	theme	water	971:975	arg1	resistance					977:986	the water resistance	967:986	the water resistance	967:986	In addition, incorporating PAL@ZnO nanorods also significantly enhanced the water resistance, and thermal stability of film.
34453980	5	9	theme	potential	1106:1114	arg1	applications					1116:1127	potential applications	1106:1127	potential applications in many fields such as food packing	1106:1163	This work provides an alternative way for the development of antibacterial films with potential applications in many fields such as food packing.
34453980	2	10	theme	inhibition	667:676	arg1	21.82 ± 0.95 mm					688:702	21.82 ± 0.95 mm	688:702	21.82 ± 0.95 mm	688:702	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	2	10	theme	inhibition	667:676	arg1	zones					678:682	inhibition zones	667:682	inhibition zones	667:682	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	2	11	theme	PAL	438:440	arg1	embedded					478:485	embedded	478:485	embedded	478:485	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	2	11	theme	PAL	438:440	arg1	nanorod					456:462	an antibacterial PAL@ZnO composite nanorod	421:462	an antibacterial PAL@ZnO composite nanorod	421:462	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	0	12	theme	film	132:135	arg1	strength					76:83	mechanical strength	65:83	mechanical strength	65:83	Multifunctional palygorskite@ZnO nanorods enhance simultaneously mechanical strength and antibacterial properties of chitosan-based film.
34453980	0	12	theme	film	132:135	arg1	properties					103:112	antibacterial properties	89:112	antibacterial properties of chitosan-based film	89:135	Multifunctional palygorskite@ZnO nanorods enhance simultaneously mechanical strength and antibacterial properties of chitosan-based film.
34453980	2	13	theme	antibacterial	424:436	arg1	embedded					478:485	embedded	478:485	embedded	478:485	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	2	13	theme	antibacterial	424:436	arg1	nanorod					456:462	an antibacterial PAL@ZnO composite nanorod	421:462	an antibacterial PAL@ZnO composite nanorod	421:462	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	0	14	theme	palygorskite	16:27	arg1	nanorods					33:40	Multifunctional palygorskite@ZnO nanorods	0:40	Multifunctional palygorskite@ZnO nanorods	0:40	Multifunctional palygorskite@ZnO nanorods enhance simultaneously mechanical strength and antibacterial properties of chitosan-based film.
34453980	2	15	theme	CS/GL	510:514	arg1	film					517:520	chitosan/gelatin (CS/GL) film	492:520	chitosan/gelatin (CS/GL) film	492:520	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	0	16	theme	Multifunctional	0:14	arg1	nanorods					33:40	Multifunctional palygorskite@ZnO nanorods	0:40	Multifunctional palygorskite@ZnO nanorods	0:40	Multifunctional palygorskite@ZnO nanorods enhance simultaneously mechanical strength and antibacterial properties of chitosan-based film.
34453980	2	17	with	films	547:551	arg1	properties					589:598	noticeably enhanced mechanical properties	558:598	noticeably enhanced mechanical properties	558:598	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	2	17	with	films	547:551	arg1	activity					618:625	antibacterial activity	604:625	antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively)	604:737	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	1	18	theme	mechanical	218:227	arg1	strength					229:236	mechanical strength	218:236	mechanical strength	218:236	A general and effective strategy was developed for improving simultaneously the mechanical strength and antibacterial performance of biopolymer-based films.
34453980	0	19	theme	ZnO	29:31	arg1	nanorods					33:40	Multifunctional palygorskite@ZnO nanorods	0:40	Multifunctional palygorskite@ZnO nanorods	0:40	Multifunctional palygorskite@ZnO nanorods enhance simultaneously mechanical strength and antibacterial properties of chitosan-based film.
34453980	2	20	theme	well-dispersed	299:312	arg1	nanoparticles					331:343	The well-dispersed zinc oxide (ZnO) nanoparticles	295:343	The well-dispersed zinc oxide (ZnO) nanoparticles	295:343	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	2	21	theme	mechanical	578:587	arg1	properties					589:598	noticeably enhanced mechanical properties	558:598	noticeably enhanced mechanical properties	558:598	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	5	22	with	films	1095:1099	arg1	applications					1116:1127	potential applications	1106:1127	potential applications in many fields such as food packing	1106:1163	This work provides an alternative way for the development of antibacterial films with potential applications in many fields such as food packing.
34453980	0	23	theme	@	28:28	arg1	nanorods					33:40	Multifunctional palygorskite@ZnO nanorods	0:40	Multifunctional palygorskite@ZnO nanorods	0:40	Multifunctional palygorskite@ZnO nanorods enhance simultaneously mechanical strength and antibacterial properties of chitosan-based film.
34453980	2	24	theme	enhanced	569:576	arg1	properties					589:598	noticeably enhanced mechanical properties	558:598	noticeably enhanced mechanical properties	558:598	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	2	25	theme	composite	446:454	arg1	embedded					478:485	embedded	478:485	embedded	478:485	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	2	25	theme	composite	446:454	arg1	nanorod					456:462	an antibacterial PAL@ZnO composite nanorod	421:462	an antibacterial PAL@ZnO composite nanorod	421:462	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	2	26	theme	palygorskite	386:397	arg1	nanorod					405:411	non-toxic natural palygorskite (PAL) nanorod	368:411	non-toxic natural palygorskite (PAL) nanorod	368:411	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	1	27	theme	antibacterial	242:254	arg1	performance					256:266	antibacterial performance	242:266	antibacterial performance	242:266	A general and effective strategy was developed for improving simultaneously the mechanical strength and antibacterial performance of biopolymer-based films.
34453980	2	28	theme	ZnO	442:444	arg1	embedded					478:485	embedded	478:485	embedded	478:485	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	2	28	theme	ZnO	442:444	arg1	nanorod					456:462	an antibacterial PAL@ZnO composite nanorod	421:462	an antibacterial PAL@ZnO composite nanorod	421:462	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	2	29	theme	chitosan/gelatin	492:507	arg1	film					517:520	chitosan/gelatin (CS/GL) film	492:520	chitosan/gelatin (CS/GL) film	492:520	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	4	30	theme	film	1014:1017	arg1	stability					1001:1009	thermal stability	993:1009	thermal stability of film	993:1017	In addition, incorporating PAL@ZnO nanorods also significantly enhanced the water resistance, and thermal stability of film.
34453980	4	30	theme	film	1014:1017	arg1	resistance					977:986	the water resistance	967:986	the water resistance	967:986	In addition, incorporating PAL@ZnO nanorods also significantly enhanced the water resistance, and thermal stability of film.
34453980	5	31	theme	antibacterial	1081:1093	arg1	films					1095:1099	antibacterial films	1081:1099	antibacterial films with potential applications in many fields such as food packing	1081:1163	This work provides an alternative way for the development of antibacterial films with potential applications in many fields such as food packing.
34453980	2	32	theme	@	441:441	arg1	embedded					478:485	embedded	478:485	embedded	478:485	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	2	32	theme	@	441:441	arg1	nanorod					456:462	an antibacterial PAL@ZnO composite nanorod	421:462	an antibacterial PAL@ZnO composite nanorod	421:462	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	0	33	theme	mechanical	65:74	arg1	strength					76:83	mechanical strength	65:83	mechanical strength	65:83	Multifunctional palygorskite@ZnO nanorods enhance simultaneously mechanical strength and antibacterial properties of chitosan-based film.
34453980	5	34	theme	food	1152:1155	arg1	packing					1157:1163	food packing	1152:1163	food packing	1152:1163	This work provides an alternative way for the development of antibacterial films with potential applications in many fields such as food packing.
34453980	3	35	theme	moisture	800:807	arg1	uptake					809:814	the moisture uptake	796:814	the moisture uptake	796:814	The toughness of films enhances to 35.13 ± 0.95 MPa and the moisture uptake decreases to 23.74 ± 0.02% after embedding 3% and 9% of PAL@ZnO, respectively.
34453980	2	36	dep	aureus	638:643	arg1	16.36 ± 1.64 mm					708:722	16.36 ± 1.64 mm	708:722	16.36 ± 1.64 mm	708:722	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	2	36	dep	aureus	638:643	arg1	21.82 ± 0.95 mm					688:702	21.82 ± 0.95 mm	688:702	21.82 ± 0.95 mm	688:702	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	2	36	dep	aureus	638:643	arg1	bacteria					657:664	bacteria	657:664	bacteria	657:664	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	2	36	dep	aureus	638:643	arg1	zones					678:682	inhibition zones	667:682	inhibition zones	667:682	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	2	37	theme	antibacterial	604:616	arg1	activity					618:625	antibacterial activity	604:625	antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively)	604:737	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	5	38	theme	alternative	1042:1052	arg1	way					1054:1056	an alternative way	1039:1056	an alternative way for the development of antibacterial films with potential applications in many fields such as food packing	1039:1163	This work provides an alternative way for the development of antibacterial films with potential applications in many fields such as food packing.
34453980	1	39	theme	general	140:146	arg1	strategy					162:169	A general and effective strategy	138:169	A general and effective strategy	138:169	A general and effective strategy was developed for improving simultaneously the mechanical strength and antibacterial performance of biopolymer-based films.
34453980	3	40	theme	@	875:875	arg1	ZnO					876:878	PAL@ZnO	872:878	PAL@ZnO	872:878	The toughness of films enhances to 35.13 ± 0.95 MPa and the moisture uptake decreases to 23.74 ± 0.02% after embedding 3% and 9% of PAL@ZnO, respectively.
34453980	4	41	theme	PAL	922:924	arg1	nanorods					930:937	PAL@ZnO nanorods	922:937	PAL@ZnO nanorods	922:937	In addition, incorporating PAL@ZnO nanorods also significantly enhanced the water resistance, and thermal stability of film.
34453980	2	42	theme	oxide	319:323	arg1	nanoparticles					331:343	The well-dispersed zinc oxide (ZnO) nanoparticles	295:343	The well-dispersed zinc oxide (ZnO) nanoparticles	295:343	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	2	43	theme	natural	378:384	arg1	nanorod					405:411	non-toxic natural palygorskite (PAL) nanorod	368:411	non-toxic natural palygorskite (PAL) nanorod	368:411	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	3	44	theme	ZnO	876:878	arg1	ZnO					876:878	PAL@ZnO	872:878	PAL@ZnO	872:878	The toughness of films enhances to 35.13 ± 0.95 MPa and the moisture uptake decreases to 23.74 ± 0.02% after embedding 3% and 9% of PAL@ZnO, respectively.
34453980	3	44	theme	ZnO	876:878	arg1	%					860:860	3%	859:860	3%	859:860	The toughness of films enhances to 35.13 ± 0.95 MPa and the moisture uptake decreases to 23.74 ± 0.02% after embedding 3% and 9% of PAL@ZnO, respectively.
34453980	3	44	theme	ZnO	876:878	arg1	%					867:867	9%	866:867	9% of PAL@ZnO	866:878	The toughness of films enhances to 35.13 ± 0.95 MPa and the moisture uptake decreases to 23.74 ± 0.02% after embedding 3% and 9% of PAL@ZnO, respectively.
34453980	2	45	theme	zinc	314:317	arg1	ZnO					326:328	ZnO	326:328	ZnO	326:328	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	2	45	theme	zinc	314:317	arg1	oxide					319:323	zinc oxide	314:323	The well-dispersed zinc oxide (ZnO) nanoparticles	295:343	The well-dispersed zinc oxide (ZnO) nanoparticles were in-situ loaded on non-toxic natural palygorskite (PAL) nanorod to form an antibacterial PAL@ZnO composite nanorod, which can be embedded into chitosan/gelatin (CS/GL) film to produce the composite films with noticeably enhanced mechanical properties and antibacterial activity against S. aureus and E. coli bacteria (inhibition zones are 21.82 ± 0.95 mm and 16.36 ± 1.64 mm, respectively).
34453980	1	46	theme	effective	152:160	arg1	strategy					162:169	A general and effective strategy	138:169	A general and effective strategy	138:169	A general and effective strategy was developed for improving simultaneously the mechanical strength and antibacterial performance of biopolymer-based films.
34453980	4	47	theme	@	925:925	arg1	nanorods					930:937	PAL@ZnO nanorods	922:937	PAL@ZnO nanorods	922:937	In addition, incorporating PAL@ZnO nanorods also significantly enhanced the water resistance, and thermal stability of film.
34453980	1	48	theme	biopolymer-based	271:286	arg1	films					288:292	biopolymer-based films	271:292	biopolymer-based films	271:292	A general and effective strategy was developed for improving simultaneously the mechanical strength and antibacterial performance of biopolymer-based films.
34453980	4	49	theme	ZnO	926:928	arg1	nanorods					930:937	PAL@ZnO nanorods	922:937	PAL@ZnO nanorods	922:937	In addition, incorporating PAL@ZnO nanorods also significantly enhanced the water resistance, and thermal stability of film.
34453980	1	50	dep	strength	229:236	arg1	the					214:216	the	214:216	the	214:216	A general and effective strategy was developed for improving simultaneously the mechanical strength and antibacterial performance of biopolymer-based films.
34453980	3	51	theme	PAL	872:874	arg1	ZnO					876:878	PAL@ZnO	872:878	PAL@ZnO	872:878	The toughness of films enhances to 35.13 ± 0.95 MPa and the moisture uptake decreases to 23.74 ± 0.02% after embedding 3% and 9% of PAL@ZnO, respectively.
34453980	1	52	theme	films	288:292	arg1	strength					229:236	mechanical strength	218:236	mechanical strength	218:236	A general and effective strategy was developed for improving simultaneously the mechanical strength and antibacterial performance of biopolymer-based films.
34453980	1	52	theme	films	288:292	arg1	performance					256:266	antibacterial performance	242:266	antibacterial performance	242:266	A general and effective strategy was developed for improving simultaneously the mechanical strength and antibacterial performance of biopolymer-based films.
34453980	5	53	theme	many	1132:1135	arg1	fields					1137:1142	many fields	1132:1142	many fields such as food packing	1132:1163	This work provides an alternative way for the development of antibacterial films with potential applications in many fields such as food packing.
34453980	5	53	theme	many	1132:1135	arg1	packing					1157:1163	food packing	1152:1163	food packing	1152:1163	This work provides an alternative way for the development of antibacterial films with potential applications in many fields such as food packing.
34453980	5	54	from	applications	1116:1127	arg1	fields					1137:1142	many fields	1132:1142	many fields such as food packing	1132:1163	This work provides an alternative way for the development of antibacterial films with potential applications in many fields such as food packing.
34453980	5	54	from	applications	1116:1127	arg1	packing					1157:1163	food packing	1152:1163	food packing	1152:1163	This work provides an alternative way for the development of antibacterial films with potential applications in many fields such as food packing.
32901572	7	0	theme	cyclodextrins	1227:1239	arg1	use					1207:1209	the use	1203:1209	the use of NO-releasing cyclodextrins as a matrix for the delivery of antibacterial and anticancer drugs in a suitable formulation	1203:1332	EXPERT OPINION The results support the use of NO-releasing cyclodextrins as a matrix for the delivery of antibacterial and anticancer drugs in a suitable formulation.
32901572	6	1	theme	0.5 μmol	1027:1034	arg1	range					1018:1022	the range	1014:1022	the range of 0.5 μmol to 2.5 μmol per milligram of functionalized cyclodextrin with a half-life for NO release in a range of between about 0.7-4.2 hours	1014:1165	The amount of NO released is in the range of 0.5 μmol to 2.5 μmol per milligram of functionalized cyclodextrin with a half-life for NO release in a range of between about 0.7-4.2 hours.
32901572	7	2	dep	OPINION	1175:1181	arg1	support					1195:1201	support	1195:1201	support the use of NO-releasing cyclodextrins as a matrix for the delivery of antibacterial and anticancer drugs in a suitable formulation	1195:1332	EXPERT OPINION The results support the use of NO-releasing cyclodextrins as a matrix for the delivery of antibacterial and anticancer drugs in a suitable formulation.
32901572	6	3	from	release	1117:1123	arg1	range					1130:1134	a range	1128:1134	a range of between about 0.7-4.2 hours	1128:1165	The amount of NO released is in the range of 0.5 μmol to 2.5 μmol per milligram of functionalized cyclodextrin with a half-life for NO release in a range of between about 0.7-4.2 hours.
32901572	3	4	theme	antibacterial	514:526	arg1	agents					543:548	an antibacterial or antitumoral agents	511:548	an antibacterial or antitumoral agents	511:548	When functionalized with nitric oxide (NO) releasing groups, and suitably loaded with an antibacterial or antitumoral agents, they can exert additive activity, especially toward certain bacterial strains and cell cancer lines.
32901572	5	5	theme	Anticancer	930:939	arg1	activity					941:948	Anticancer activity	930:948	Anticancer activity	930:948	Anticancer activity is measured against A549 cells.
32901572	7	6	theme	suitable	1313:1320	arg1	formulation					1322:1332	a suitable formulation	1311:1332	a suitable formulation	1311:1332	EXPERT OPINION The results support the use of NO-releasing cyclodextrins as a matrix for the delivery of antibacterial and anticancer drugs in a suitable formulation.
32901572	4	7	theme	NO-releasing	689:700	arg1	cyclodextrins					702:714	NO-releasing cyclodextrins	689:714	NO-releasing cyclodextrins	689:714	AREAS COVERED US2019343869 describes NO-releasing cyclodextrins, a method for their synthesis, a composition that is based on them, and their application as anticancer or antibacterial agents, especially toward planktonic P. aeruginosa and the biofilm resulting from infection.
32901572	0	8	theme	US2019343869	102:113	arg1	evaluation					88:97	a patent evaluation	79:97	Nitric oxide-releasing cyclodextrins as biodegradable antibacterial scaffolds: a patent evaluation of US2019343869(A1).	0:118	Nitric oxide-releasing cyclodextrins as biodegradable antibacterial scaffolds: a patent evaluation of US2019343869(A1).
32901572	7	9	from	delivery	1261:1268	arg1	formulation					1322:1332	a suitable formulation	1311:1332	a suitable formulation	1311:1332	EXPERT OPINION The results support the use of NO-releasing cyclodextrins as a matrix for the delivery of antibacterial and anticancer drugs in a suitable formulation.
32901572	8	10	theme	antibacterial	1344:1356	arg1	activity					1358:1365	antibacterial activity	1344:1365	antibacterial activity	1344:1365	However, antibacterial activity seems to be weak, and more focused studies are needed.
32901572	1	11	theme	major	172:176	arg1	scourges					178:185	the major scourges	168:185	the major scourges for health care worldwide	168:211	INTRODUCTION Antimicrobial resistance is one of the major scourges for health care worldwide; therefore, novel investigational approaches are needed to potentiate and preserve the current antibacterial arsenal.
32901572	1	12	theme	scourges	178:185	arg1	one					161:163	one	161:163	one	161:163	INTRODUCTION Antimicrobial resistance is one of the major scourges for health care worldwide; therefore, novel investigational approaches are needed to potentiate and preserve the current antibacterial arsenal.
32901572	1	12	theme	scourges	178:185	arg1	scourges					178:185	the major scourges	168:185	the major scourges for health care worldwide	168:211	INTRODUCTION Antimicrobial resistance is one of the major scourges for health care worldwide; therefore, novel investigational approaches are needed to potentiate and preserve the current antibacterial arsenal.
32901572	0	13	theme	oxide-releasing	7:21	arg1	cyclodextrins					23:35	Nitric oxide-releasing cyclodextrins	0:35	Nitric oxide-releasing cyclodextrins as biodegradable antibacterial scaffolds: a patent evaluation of US2019343869(A1).	0:118	Nitric oxide-releasing cyclodextrins as biodegradable antibacterial scaffolds: a patent evaluation of US2019343869(A1).
32901572	5	14	theme	A549	970:973	arg1	cells					975:979	A549 cells	970:979	A549 cells	970:979	Anticancer activity is measured against A549 cells.
32901572	2	15	theme	therapeutic	405:415	arg1	agents					417:422	several different therapeutic agents	387:422	several different therapeutic agents	387:422	Cyclodextrins are known to improve the formulability of several different therapeutic agents.
32901572	0	16	theme	Nitric	0:5	arg1	cyclodextrins					23:35	Nitric oxide-releasing cyclodextrins	0:35	Nitric oxide-releasing cyclodextrins as biodegradable antibacterial scaffolds: a patent evaluation of US2019343869(A1).	0:118	Nitric oxide-releasing cyclodextrins as biodegradable antibacterial scaffolds: a patent evaluation of US2019343869(A1).
32901572	7	17	theme	EXPERT	1168:1173	arg1	OPINION					1175:1181	EXPERT OPINION	1168:1181	EXPERT OPINION The results support the use of NO-releasing cyclodextrins as a matrix for the delivery of antibacterial and anticancer drugs in a suitable formulation.	1168:1333	EXPERT OPINION The results support the use of NO-releasing cyclodextrins as a matrix for the delivery of antibacterial and anticancer drugs in a suitable formulation.
32901572	2	18	theme	different	395:403	arg1	agents					417:422	several different therapeutic agents	387:422	several different therapeutic agents	387:422	Cyclodextrins are known to improve the formulability of several different therapeutic agents.
32901572	6	19	theme	NO	996:997	arg1	NO					996:997	NO	996:997	NO	996:997	The amount of NO released is in the range of 0.5 μmol to 2.5 μmol per milligram of functionalized cyclodextrin with a half-life for NO release in a range of between about 0.7-4.2 hours.
32901572	6	19	theme	NO	996:997	arg1	amount					986:991	The amount	982:991	The amount of NO released	982:1006	The amount of NO released is in the range of 0.5 μmol to 2.5 μmol per milligram of functionalized cyclodextrin with a half-life for NO release in a range of between about 0.7-4.2 hours.
32901572	3	20	theme	additive	566:573	arg1	activity					575:582	additive activity	566:582	additive activity	566:582	When functionalized with nitric oxide (NO) releasing groups, and suitably loaded with an antibacterial or antitumoral agents, they can exert additive activity, especially toward certain bacterial strains and cell cancer lines.
32901572	4	21	dep	US2019343869	666:677	arg1	describes					679:687	describes	679:687	describes	679:687	AREAS COVERED US2019343869 describes NO-releasing cyclodextrins, a method for their synthesis, a composition that is based on them, and their application as anticancer or antibacterial agents, especially toward planktonic P. aeruginosa and the biofilm resulting from infection.
32901572	1	22	theme	care	198:201	arg1	worldwide					203:211	health care worldwide	191:211	health care worldwide	191:211	INTRODUCTION Antimicrobial resistance is one of the major scourges for health care worldwide; therefore, novel investigational approaches are needed to potentiate and preserve the current antibacterial arsenal.
32901572	3	23	theme	antitumoral	531:541	arg1	agents					543:548	an antibacterial or antitumoral agents	511:548	an antibacterial or antitumoral agents	511:548	When functionalized with nitric oxide (NO) releasing groups, and suitably loaded with an antibacterial or antitumoral agents, they can exert additive activity, especially toward certain bacterial strains and cell cancer lines.
32901572	0	24	theme	antibacterial	54:66	arg1	scaffolds					68:76	biodegradable antibacterial scaffolds	40:76	biodegradable antibacterial scaffolds	40:76	Nitric oxide-releasing cyclodextrins as biodegradable antibacterial scaffolds: a patent evaluation of US2019343869(A1).
32901572	1	25	theme	current	300:306	arg1	arsenal					322:328	the current antibacterial arsenal	296:328	the current antibacterial arsenal	296:328	INTRODUCTION Antimicrobial resistance is one of the major scourges for health care worldwide; therefore, novel investigational approaches are needed to potentiate and preserve the current antibacterial arsenal.
32901572	6	26	with	cyclodextrin	1080:1091	arg1	half-life					1100:1108	a half-life	1098:1108	a half-life for NO release in a range of between about 0.7-4.2 hours	1098:1165	The amount of NO released is in the range of 0.5 μmol to 2.5 μmol per milligram of functionalized cyclodextrin with a half-life for NO release in a range of between about 0.7-4.2 hours.
32901572	3	27	theme	bacterial	611:619	arg1	strains					621:627	certain bacterial strains	603:627	certain bacterial strains	603:627	When functionalized with nitric oxide (NO) releasing groups, and suitably loaded with an antibacterial or antitumoral agents, they can exert additive activity, especially toward certain bacterial strains and cell cancer lines.
32901572	1	28	theme	health	191:196	arg1	worldwide					203:211	health care worldwide	191:211	health care worldwide	191:211	INTRODUCTION Antimicrobial resistance is one of the major scourges for health care worldwide; therefore, novel investigational approaches are needed to potentiate and preserve the current antibacterial arsenal.
32901572	0	29	theme	biodegradable	40:52	arg1	scaffolds					68:76	biodegradable antibacterial scaffolds	40:76	biodegradable antibacterial scaffolds	40:76	Nitric oxide-releasing cyclodextrins as biodegradable antibacterial scaffolds: a patent evaluation of US2019343869(A1).
32901572	1	30	theme	antibacterial	308:320	arg1	arsenal					322:328	the current antibacterial arsenal	296:328	the current antibacterial arsenal	296:328	INTRODUCTION Antimicrobial resistance is one of the major scourges for health care worldwide; therefore, novel investigational approaches are needed to potentiate and preserve the current antibacterial arsenal.
32901572	3	31	theme	nitric	450:455	arg1	NO					464:465	NO	464:465	NO	464:465	When functionalized with nitric oxide (NO) releasing groups, and suitably loaded with an antibacterial or antitumoral agents, they can exert additive activity, especially toward certain bacterial strains and cell cancer lines.
32901572	3	31	theme	nitric	450:455	arg1	oxide					457:461	nitric oxide	450:461	nitric oxide (NO) releasing groups	450:483	When functionalized with nitric oxide (NO) releasing groups, and suitably loaded with an antibacterial or antitumoral agents, they can exert additive activity, especially toward certain bacterial strains and cell cancer lines.
32901572	4	32	theme	antibacterial	823:835	arg1	agents					837:842	anticancer or antibacterial agents	809:842	agents	837:842	AREAS COVERED US2019343869 describes NO-releasing cyclodextrins, a method for their synthesis, a composition that is based on them, and their application as anticancer or antibacterial agents, especially toward planktonic P. aeruginosa and the biofilm resulting from infection.
32901572	7	33	theme	anticancer	1291:1300	arg1	drugs					1302:1306	antibacterial and anticancer drugs	1273:1306	drugs	1302:1306	EXPERT OPINION The results support the use of NO-releasing cyclodextrins as a matrix for the delivery of antibacterial and anticancer drugs in a suitable formulation.
32901572	6	34	theme	cyclodextrin	1080:1091	arg1	milligram					1052:1060	milligram	1052:1060	milligram of functionalized cyclodextrin with a half-life for NO release in a range of between about 0.7-4.2 hours	1052:1165	The amount of NO released is in the range of 0.5 μmol to 2.5 μmol per milligram of functionalized cyclodextrin with a half-life for NO release in a range of between about 0.7-4.2 hours.
32901572	3	35	theme	certain	603:609	arg1	strains					621:627	certain bacterial strains	603:627	certain bacterial strains	603:627	When functionalized with nitric oxide (NO) releasing groups, and suitably loaded with an antibacterial or antitumoral agents, they can exert additive activity, especially toward certain bacterial strains and cell cancer lines.
32901572	1	36	theme	INTRODUCTION	120:131	arg1	resistance					147:156	INTRODUCTION Antimicrobial resistance	120:156	INTRODUCTION Antimicrobial resistance	120:156	INTRODUCTION Antimicrobial resistance is one of the major scourges for health care worldwide; therefore, novel investigational approaches are needed to potentiate and preserve the current antibacterial arsenal.
32901572	4	37	theme	anticancer	809:818	arg1	agents					837:842	anticancer or antibacterial agents	809:842	agents	837:842	AREAS COVERED US2019343869 describes NO-releasing cyclodextrins, a method for their synthesis, a composition that is based on them, and their application as anticancer or antibacterial agents, especially toward planktonic P. aeruginosa and the biofilm resulting from infection.
32901572	3	38	theme	cancer	638:643	arg1	lines					645:649	cell cancer lines	633:649	cell cancer lines	633:649	When functionalized with nitric oxide (NO) releasing groups, and suitably loaded with an antibacterial or antitumoral agents, they can exert additive activity, especially toward certain bacterial strains and cell cancer lines.
32901572	8	39	theme	focused	1394:1400	arg1	studies					1402:1408	more focused studies	1389:1408	more focused studies	1389:1408	However, antibacterial activity seems to be weak, and more focused studies are needed.
32901572	1	40	theme	Antimicrobial	133:145	arg1	resistance					147:156	INTRODUCTION Antimicrobial resistance	120:156	INTRODUCTION Antimicrobial resistance	120:156	INTRODUCTION Antimicrobial resistance is one of the major scourges for health care worldwide; therefore, novel investigational approaches are needed to potentiate and preserve the current antibacterial arsenal.
32901572	7	41	theme	drugs	1302:1306	arg1	delivery					1261:1268	the delivery	1257:1268	the delivery of antibacterial and anticancer drugs in a suitable formulation	1257:1332	EXPERT OPINION The results support the use of NO-releasing cyclodextrins as a matrix for the delivery of antibacterial and anticancer drugs in a suitable formulation.
32901572	0	42	dep	cyclodextrins	23:35	arg1	evaluation					88:97	a patent evaluation	79:97	Nitric oxide-releasing cyclodextrins as biodegradable antibacterial scaffolds: a patent evaluation of US2019343869(A1).	0:118	Nitric oxide-releasing cyclodextrins as biodegradable antibacterial scaffolds: a patent evaluation of US2019343869(A1).
32901572	1	43	theme	novel	225:229	arg1	approaches					247:256	novel investigational approaches	225:256	novel investigational approaches	225:256	INTRODUCTION Antimicrobial resistance is one of the major scourges for health care worldwide; therefore, novel investigational approaches are needed to potentiate and preserve the current antibacterial arsenal.
32901572	0	44	theme	patent	81:86	arg1	evaluation					88:97	a patent evaluation	79:97	Nitric oxide-releasing cyclodextrins as biodegradable antibacterial scaffolds: a patent evaluation of US2019343869(A1).	0:118	Nitric oxide-releasing cyclodextrins as biodegradable antibacterial scaffolds: a patent evaluation of US2019343869(A1).
32901572	6	45	theme	functionalized	1065:1078	arg1	cyclodextrin					1080:1091	functionalized cyclodextrin	1065:1091	functionalized cyclodextrin with a half-life for NO release in a range of between about 0.7-4.2 hours	1065:1165	The amount of NO released is in the range of 0.5 μmol to 2.5 μmol per milligram of functionalized cyclodextrin with a half-life for NO release in a range of between about 0.7-4.2 hours.
32901572	2	46	theme	several	387:393	arg1	agents					417:422	several different therapeutic agents	387:422	several different therapeutic agents	387:422	Cyclodextrins are known to improve the formulability of several different therapeutic agents.
32901572	2	47	theme	agents	417:422	arg1	formulability					370:382	the formulability	366:382	the formulability of several different therapeutic agents	366:422	Cyclodextrins are known to improve the formulability of several different therapeutic agents.
32901572	1	48	theme	investigational	231:245	arg1	approaches					247:256	novel investigational approaches	225:256	novel investigational approaches	225:256	INTRODUCTION Antimicrobial resistance is one of the major scourges for health care worldwide; therefore, novel investigational approaches are needed to potentiate and preserve the current antibacterial arsenal.
32901572	3	49	theme	cell	633:636	arg1	lines					645:649	cell cancer lines	633:649	cell cancer lines	633:649	When functionalized with nitric oxide (NO) releasing groups, and suitably loaded with an antibacterial or antitumoral agents, they can exert additive activity, especially toward certain bacterial strains and cell cancer lines.
32901572	7	50	theme	antibacterial	1273:1285	arg1	drugs					1302:1306	antibacterial and anticancer drugs	1273:1306	drugs	1302:1306	EXPERT OPINION The results support the use of NO-releasing cyclodextrins as a matrix for the delivery of antibacterial and anticancer drugs in a suitable formulation.
32901572	7	51	theme	NO-releasing	1214:1225	arg1	cyclodextrins					1227:1239	NO-releasing cyclodextrins	1214:1239	NO-releasing cyclodextrins	1214:1239	EXPERT OPINION The results support the use of NO-releasing cyclodextrins as a matrix for the delivery of antibacterial and anticancer drugs in a suitable formulation.
32487414	7	0	from	CS	852:853	arg1	AgNPs					835:839	AgNPs	835:839	AgNPs from AgNPs@CS	835:853	Notably, the accumulative release rate of AgNPs from AgNPs@CS was 10.2% in 9 days, while that from CNF/AgNPs@CS paper was only 6.7% due to the anchoring effect of both CS and CNF, which was low for avoiding the cumulative toxicity problem.
32487414	7	0	from	CS	852:853	arg1	rate					827:830	the accumulative release rate	802:830	the accumulative release rate of AgNPs from AgNPs@CS	802:853	Notably, the accumulative release rate of AgNPs from AgNPs@CS was 10.2% in 9 days, while that from CNF/AgNPs@CS paper was only 6.7% due to the anchoring effect of both CS and CNF, which was low for avoiding the cumulative toxicity problem.
32487414	7	0	from	CS	852:853	arg1	%					863:863	10.2%	859:863	10.2%	859:863	Notably, the accumulative release rate of AgNPs from AgNPs@CS was 10.2% in 9 days, while that from CNF/AgNPs@CS paper was only 6.7% due to the anchoring effect of both CS and CNF, which was low for avoiding the cumulative toxicity problem.
32487414	8	1	theme	oxygen	1257:1262	arg1	rate					1277:1280	oxygen transmission rate	1257:1280	oxygen transmission rate	1257:1280	In addition, the mechanical and barrier properties of CNF/AgNPs@CS paper were also improved by 29.4% (tensile index), 2.7% (tear index), 7.4% (burst index), 10% (folding endurance), 0.8% (water vapor transmission) and 9.4% (oxygen transmission rate), respectively.
32487414	8	1	theme	oxygen	1257:1262	arg1	%					1254:1254	9.4%	1251:1254	9.4% (oxygen transmission rate)	1251:1281	In addition, the mechanical and barrier properties of CNF/AgNPs@CS paper were also improved by 29.4% (tensile index), 2.7% (tear index), 7.4% (burst index), 10% (folding endurance), 0.8% (water vapor transmission) and 9.4% (oxygen transmission rate), respectively.
32487414	6	2	theme	@	708:708	arg1	paper					712:716	The obtained CNF/AgNPs@CS paper	686:716	The obtained CNF/AgNPs@CS paper	686:716	The obtained CNF/AgNPs@CS paper exhibited superior antibacterial properties against E. coli and S. aureus.
32487414	1	3	theme	due	155:157	arg1	sterilization					141:153	sterilization	141:153	sterilization due to their broad-spectrum bactericidal properties	141:205	Silver nanoparticles (AgNPs) have been widely used for sterilization due to their broad-spectrum bactericidal properties.
32487414	7	4	from	rate	827:830	arg1	CS					852:853	AgNPs@CS	846:853	AgNPs@CS	846:853	Notably, the accumulative release rate of AgNPs from AgNPs@CS was 10.2% in 9 days, while that from CNF/AgNPs@CS paper was only 6.7% due to the anchoring effect of both CS and CNF, which was low for avoiding the cumulative toxicity problem.
32487414	4	5	theme	corn	519:522	arg1	stalk					524:528	corn stalk	519:528	corn stalk	519:528	The synthesis involves with silver ammonia solution as Ag precursor, and corn stalk as green reducing agent and carbon precursor.
32487414	9	6	theme	medical	1360:1366	arg1	material					1382:1389	a medical antibacterial material	1358:1389	a medical antibacterial material	1358:1389	Therefore, the composite paper has potential application as a medical antibacterial material.
32487414	9	6	theme	medical	1360:1366	arg1	application					1343:1353	potential application	1333:1353	potential application	1333:1353	Therefore, the composite paper has potential application as a medical antibacterial material.
32487414	5	7	theme	@	594:594	arg1	CS					595:596	AgNPs@CS	589:596	AgNPs@CS	589:596	Furthermore, AgNPs@CS was anchored by cellulose nanofibers (CNF) to obtain the antibacterial composite paper.
32487414	7	8	theme	@	851:851	arg1	CS					852:853	AgNPs@CS	846:853	AgNPs@CS	846:853	Notably, the accumulative release rate of AgNPs from AgNPs@CS was 10.2% in 9 days, while that from CNF/AgNPs@CS paper was only 6.7% due to the anchoring effect of both CS and CNF, which was low for avoiding the cumulative toxicity problem.
32487414	7	9	theme	cumulative	1004:1013	arg1	toxicity					1015:1022	the cumulative toxicity	1000:1022	the cumulative toxicity problem	1000:1030	Notably, the accumulative release rate of AgNPs from AgNPs@CS was 10.2% in 9 days, while that from CNF/AgNPs@CS paper was only 6.7% due to the anchoring effect of both CS and CNF, which was low for avoiding the cumulative toxicity problem.
32487414	3	10	theme	silver	413:418	arg1	CS					441:442	AgNPs@CS	435:442	AgNPs@CS	435:442	This study proposed a one-pot hydrothermal strategy to synthesize carbon spheres immobilized silver nanoparticles (AgNPs@CS).
32487414	3	10	theme	silver	413:418	arg1	nanoparticles					420:432	carbon spheres immobilized silver nanoparticles	386:432	carbon spheres immobilized silver nanoparticles (AgNPs@CS)	386:443	This study proposed a one-pot hydrothermal strategy to synthesize carbon spheres immobilized silver nanoparticles (AgNPs@CS).
32487414	7	11	from	%	863:863	arg1	9 days					868:873	9 days	868:873	9 days	868:873	Notably, the accumulative release rate of AgNPs from AgNPs@CS was 10.2% in 9 days, while that from CNF/AgNPs@CS paper was only 6.7% due to the anchoring effect of both CS and CNF, which was low for avoiding the cumulative toxicity problem.
32487414	7	12	theme	toxicity	1015:1022	arg1	problem					1024:1030	the cumulative toxicity problem	1000:1030	the cumulative toxicity problem	1000:1030	Notably, the accumulative release rate of AgNPs from AgNPs@CS was 10.2% in 9 days, while that from CNF/AgNPs@CS paper was only 6.7% due to the anchoring effect of both CS and CNF, which was low for avoiding the cumulative toxicity problem.
32487414	7	13	theme	@	901:901	arg1	paper					905:909	CNF/AgNPs@CS paper	892:909	CNF/AgNPs@CS paper	892:909	Notably, the accumulative release rate of AgNPs from AgNPs@CS was 10.2% in 9 days, while that from CNF/AgNPs@CS paper was only 6.7% due to the anchoring effect of both CS and CNF, which was low for avoiding the cumulative toxicity problem.
32487414	2	14	theme	free	296:299	arg1	AgNPs					301:305	free AgNPs	296:305	free AgNPs	296:305	However, there exist the problems of premature releasing and accumulative toxicity when free AgNPs are applied.
32487414	6	15	theme	CNF/AgNPs	699:707	arg1	paper					712:716	The obtained CNF/AgNPs@CS paper	686:716	The obtained CNF/AgNPs@CS paper	686:716	The obtained CNF/AgNPs@CS paper exhibited superior antibacterial properties against E. coli and S. aureus.
32487414	3	16	theme	hydrothermal	350:361	arg1	strategy					363:370	a one-pot hydrothermal strategy	340:370	a one-pot hydrothermal strategy to synthesize carbon spheres immobilized silver nanoparticles (AgNPs@CS)	340:443	This study proposed a one-pot hydrothermal strategy to synthesize carbon spheres immobilized silver nanoparticles (AgNPs@CS).
32487414	4	17	theme	ammonia	481:487	arg1	solution					489:496	silver ammonia solution	474:496	silver ammonia solution	474:496	The synthesis involves with silver ammonia solution as Ag precursor, and corn stalk as green reducing agent and carbon precursor.
32487414	7	18	theme	CS	902:903	arg1	paper					905:909	CNF/AgNPs@CS paper	892:909	CNF/AgNPs@CS paper	892:909	Notably, the accumulative release rate of AgNPs from AgNPs@CS was 10.2% in 9 days, while that from CNF/AgNPs@CS paper was only 6.7% due to the anchoring effect of both CS and CNF, which was low for avoiding the cumulative toxicity problem.
32487414	9	19	theme	composite	1313:1321	arg1	paper					1323:1327	the composite paper	1309:1327	the composite paper	1309:1327	Therefore, the composite paper has potential application as a medical antibacterial material.
32487414	6	20	theme	obtained	690:697	arg1	paper					712:716	The obtained CNF/AgNPs@CS paper	686:716	The obtained CNF/AgNPs@CS paper	686:716	The obtained CNF/AgNPs@CS paper exhibited superior antibacterial properties against E. coli and S. aureus.
32487414	8	21	theme	barrier	1065:1071	arg1	properties					1073:1082	the mechanical and barrier properties	1046:1082	properties	1073:1082	In addition, the mechanical and barrier properties of CNF/AgNPs@CS paper were also improved by 29.4% (tensile index), 2.7% (tear index), 7.4% (burst index), 10% (folding endurance), 0.8% (water vapor transmission) and 9.4% (oxygen transmission rate), respectively.
32487414	4	22	theme	Ag	501:502	arg1	precursor					504:512	Ag precursor	501:512	Ag precursor	501:512	The synthesis involves with silver ammonia solution as Ag precursor, and corn stalk as green reducing agent and carbon precursor.
32487414	0	23	theme	stalk-based	40:50	arg1	spheres					59:65	corn stalk-based carbon spheres	35:65	corn stalk-based carbon spheres	35:65	Antibacterial composite paper with corn stalk-based carbon spheres immobilized AgNPs.
32487414	1	24	theme	Silver	86:91	arg1	AgNPs					108:112	AgNPs	108:112	AgNPs	108:112	Silver nanoparticles (AgNPs) have been widely used for sterilization due to their broad-spectrum bactericidal properties.
32487414	1	24	theme	Silver	86:91	arg1	nanoparticles					93:105	Silver nanoparticles	86:105	Silver nanoparticles (AgNPs)	86:113	Silver nanoparticles (AgNPs) have been widely used for sterilization due to their broad-spectrum bactericidal properties.
32487414	1	25	theme	broad-spectrum	168:181	arg1	properties					196:205	their broad-spectrum bactericidal properties	162:205	their broad-spectrum bactericidal properties	162:205	Silver nanoparticles (AgNPs) have been widely used for sterilization due to their broad-spectrum bactericidal properties.
32487414	0	26	theme	composite	14:22	arg1	paper					24:28	Antibacterial composite paper	0:28	Antibacterial composite paper with corn stalk-based carbon spheres	0:65	Antibacterial composite paper with corn stalk-based carbon spheres immobilized AgNPs.
32487414	7	27	theme	CNF/AgNPs	892:900	arg1	paper					905:909	CNF/AgNPs@CS paper	892:909	CNF/AgNPs@CS paper	892:909	Notably, the accumulative release rate of AgNPs from AgNPs@CS was 10.2% in 9 days, while that from CNF/AgNPs@CS paper was only 6.7% due to the anchoring effect of both CS and CNF, which was low for avoiding the cumulative toxicity problem.
32487414	1	28	used	used	132:135	arg2	AgNPs					108:112	AgNPs	108:112	AgNPs	108:112	Silver nanoparticles (AgNPs) have been widely used for sterilization due to their broad-spectrum bactericidal properties.
32487414	1	28	used	used	132:135	arg2	nanoparticles					93:105	Silver nanoparticles	86:105	Silver nanoparticles (AgNPs)	86:113	Silver nanoparticles (AgNPs) have been widely used for sterilization due to their broad-spectrum bactericidal properties.
32487414	7	29	theme	AgNPs	835:839	arg1	rate					827:830	the accumulative release rate	802:830	the accumulative release rate of AgNPs from AgNPs@CS	802:853	Notably, the accumulative release rate of AgNPs from AgNPs@CS was 10.2% in 9 days, while that from CNF/AgNPs@CS paper was only 6.7% due to the anchoring effect of both CS and CNF, which was low for avoiding the cumulative toxicity problem.
32487414	7	29	theme	AgNPs	835:839	arg1	%					863:863	10.2%	859:863	10.2%	859:863	Notably, the accumulative release rate of AgNPs from AgNPs@CS was 10.2% in 9 days, while that from CNF/AgNPs@CS paper was only 6.7% due to the anchoring effect of both CS and CNF, which was low for avoiding the cumulative toxicity problem.
32487414	1	30	theme	bactericidal	183:194	arg1	properties					196:205	their broad-spectrum bactericidal properties	162:205	their broad-spectrum bactericidal properties	162:205	Silver nanoparticles (AgNPs) have been widely used for sterilization due to their broad-spectrum bactericidal properties.
32487414	0	31	theme	Antibacterial	0:12	arg1	paper					24:28	Antibacterial composite paper	0:28	Antibacterial composite paper with corn stalk-based carbon spheres	0:65	Antibacterial composite paper with corn stalk-based carbon spheres immobilized AgNPs.
32487414	9	32	theme	potential	1333:1341	arg1	material					1382:1389	a medical antibacterial material	1358:1389	a medical antibacterial material	1358:1389	Therefore, the composite paper has potential application as a medical antibacterial material.
32487414	9	32	theme	potential	1333:1341	arg1	application					1343:1353	potential application	1333:1353	potential application	1333:1353	Therefore, the composite paper has potential application as a medical antibacterial material.
32487414	7	33	theme	release	819:825	arg1	rate					827:830	the accumulative release rate	802:830	the accumulative release rate of AgNPs from AgNPs@CS	802:853	Notably, the accumulative release rate of AgNPs from AgNPs@CS was 10.2% in 9 days, while that from CNF/AgNPs@CS paper was only 6.7% due to the anchoring effect of both CS and CNF, which was low for avoiding the cumulative toxicity problem.
32487414	7	33	theme	release	819:825	arg1	%					863:863	10.2%	859:863	10.2%	859:863	Notably, the accumulative release rate of AgNPs from AgNPs@CS was 10.2% in 9 days, while that from CNF/AgNPs@CS paper was only 6.7% due to the anchoring effect of both CS and CNF, which was low for avoiding the cumulative toxicity problem.
32487414	3	34	theme	carbon	386:391	arg1	CS					441:442	AgNPs@CS	435:442	AgNPs@CS	435:442	This study proposed a one-pot hydrothermal strategy to synthesize carbon spheres immobilized silver nanoparticles (AgNPs@CS).
32487414	3	34	theme	carbon	386:391	arg1	nanoparticles					420:432	carbon spheres immobilized silver nanoparticles	386:432	carbon spheres immobilized silver nanoparticles (AgNPs@CS)	386:443	This study proposed a one-pot hydrothermal strategy to synthesize carbon spheres immobilized silver nanoparticles (AgNPs@CS).
32487414	9	35	contain	has	1329:1331	arg2	application					1343:1353	potential application	1333:1353	potential application	1333:1353	Therefore, the composite paper has potential application as a medical antibacterial material.
32487414	9	35	contain	has	1329:1331	arg1	paper					1323:1327	the composite paper	1309:1327	the composite paper	1309:1327	Therefore, the composite paper has potential application as a medical antibacterial material.
32487414	9	35	contain	has	1329:1331	arg2	material					1382:1389	a medical antibacterial material	1358:1389	a medical antibacterial material	1358:1389	Therefore, the composite paper has potential application as a medical antibacterial material.
32487414	5	36	theme	AgNPs	589:593	arg1	CS					595:596	AgNPs@CS	589:596	AgNPs@CS	589:596	Furthermore, AgNPs@CS was anchored by cellulose nanofibers (CNF) to obtain the antibacterial composite paper.
32487414	3	37	theme	spheres	393:399	arg1	CS					441:442	AgNPs@CS	435:442	AgNPs@CS	435:442	This study proposed a one-pot hydrothermal strategy to synthesize carbon spheres immobilized silver nanoparticles (AgNPs@CS).
32487414	3	37	theme	spheres	393:399	arg1	nanoparticles					420:432	carbon spheres immobilized silver nanoparticles	386:432	carbon spheres immobilized silver nanoparticles (AgNPs@CS)	386:443	This study proposed a one-pot hydrothermal strategy to synthesize carbon spheres immobilized silver nanoparticles (AgNPs@CS).
32487414	8	38	theme	vapor	1227:1231	arg1	transmission					1233:1244	water vapor transmission	1221:1244	water vapor transmission	1221:1244	In addition, the mechanical and barrier properties of CNF/AgNPs@CS paper were also improved by 29.4% (tensile index), 2.7% (tear index), 7.4% (burst index), 10% (folding endurance), 0.8% (water vapor transmission) and 9.4% (oxygen transmission rate), respectively.
32487414	8	38	theme	vapor	1227:1231	arg1	%					1218:1218	0.8%	1215:1218	0.8% (water vapor transmission)	1215:1245	In addition, the mechanical and barrier properties of CNF/AgNPs@CS paper were also improved by 29.4% (tensile index), 2.7% (tear index), 7.4% (burst index), 10% (folding endurance), 0.8% (water vapor transmission) and 9.4% (oxygen transmission rate), respectively.
32487414	8	39	theme	tensile	1135:1141	arg1	%					1132:1132	29.4%	1128:1132	29.4% (tensile index)	1128:1148	In addition, the mechanical and barrier properties of CNF/AgNPs@CS paper were also improved by 29.4% (tensile index), 2.7% (tear index), 7.4% (burst index), 10% (folding endurance), 0.8% (water vapor transmission) and 9.4% (oxygen transmission rate), respectively.
32487414	8	39	theme	tensile	1135:1141	arg1	index					1143:1147	tensile index	1135:1147	tensile index	1135:1147	In addition, the mechanical and barrier properties of CNF/AgNPs@CS paper were also improved by 29.4% (tensile index), 2.7% (tear index), 7.4% (burst index), 10% (folding endurance), 0.8% (water vapor transmission) and 9.4% (oxygen transmission rate), respectively.
32487414	3	40	theme	AgNPs	435:439	arg1	CS					441:442	AgNPs@CS	435:442	AgNPs@CS	435:442	This study proposed a one-pot hydrothermal strategy to synthesize carbon spheres immobilized silver nanoparticles (AgNPs@CS).
32487414	3	40	theme	AgNPs	435:439	arg1	nanoparticles					420:432	carbon spheres immobilized silver nanoparticles	386:432	carbon spheres immobilized silver nanoparticles (AgNPs@CS)	386:443	This study proposed a one-pot hydrothermal strategy to synthesize carbon spheres immobilized silver nanoparticles (AgNPs@CS).
32487414	4	41	theme	carbon	558:563	arg1	precursor					565:573	carbon precursor	558:573	carbon precursor	558:573	The synthesis involves with silver ammonia solution as Ag precursor, and corn stalk as green reducing agent and carbon precursor.
32487414	7	42	theme	CNF	968:970	arg1	low					983:985	low	983:985	low	983:985	Notably, the accumulative release rate of AgNPs from AgNPs@CS was 10.2% in 9 days, while that from CNF/AgNPs@CS paper was only 6.7% due to the anchoring effect of both CS and CNF, which was low for avoiding the cumulative toxicity problem.
32487414	7	42	theme	CNF	968:970	arg1	effect					946:951	the anchoring effect	932:951	the anchoring effect	932:951	Notably, the accumulative release rate of AgNPs from AgNPs@CS was 10.2% in 9 days, while that from CNF/AgNPs@CS paper was only 6.7% due to the anchoring effect of both CS and CNF, which was low for avoiding the cumulative toxicity problem.
32487414	8	43	theme	water	1221:1225	arg1	transmission					1233:1244	water vapor transmission	1221:1244	water vapor transmission	1221:1244	In addition, the mechanical and barrier properties of CNF/AgNPs@CS paper were also improved by 29.4% (tensile index), 2.7% (tear index), 7.4% (burst index), 10% (folding endurance), 0.8% (water vapor transmission) and 9.4% (oxygen transmission rate), respectively.
32487414	8	43	theme	water	1221:1225	arg1	%					1218:1218	0.8%	1215:1218	0.8% (water vapor transmission)	1215:1245	In addition, the mechanical and barrier properties of CNF/AgNPs@CS paper were also improved by 29.4% (tensile index), 2.7% (tear index), 7.4% (burst index), 10% (folding endurance), 0.8% (water vapor transmission) and 9.4% (oxygen transmission rate), respectively.
32487414	8	44	theme	folding	1195:1201	arg1	endurance					1203:1211	folding endurance	1195:1211	folding endurance	1195:1211	In addition, the mechanical and barrier properties of CNF/AgNPs@CS paper were also improved by 29.4% (tensile index), 2.7% (tear index), 7.4% (burst index), 10% (folding endurance), 0.8% (water vapor transmission) and 9.4% (oxygen transmission rate), respectively.
32487414	8	44	theme	folding	1195:1201	arg1	%					1192:1192	10%	1190:1192	10% (folding endurance)	1190:1212	In addition, the mechanical and barrier properties of CNF/AgNPs@CS paper were also improved by 29.4% (tensile index), 2.7% (tear index), 7.4% (burst index), 10% (folding endurance), 0.8% (water vapor transmission) and 9.4% (oxygen transmission rate), respectively.
32487414	7	45	theme	accumulative	806:817	arg1	rate					827:830	the accumulative release rate	802:830	the accumulative release rate of AgNPs from AgNPs@CS	802:853	Notably, the accumulative release rate of AgNPs from AgNPs@CS was 10.2% in 9 days, while that from CNF/AgNPs@CS paper was only 6.7% due to the anchoring effect of both CS and CNF, which was low for avoiding the cumulative toxicity problem.
32487414	7	45	theme	accumulative	806:817	arg1	%					863:863	10.2%	859:863	10.2%	859:863	Notably, the accumulative release rate of AgNPs from AgNPs@CS was 10.2% in 9 days, while that from CNF/AgNPs@CS paper was only 6.7% due to the anchoring effect of both CS and CNF, which was low for avoiding the cumulative toxicity problem.
32487414	0	46	theme	corn	35:38	arg1	spheres					59:65	corn stalk-based carbon spheres	35:65	corn stalk-based carbon spheres	35:65	Antibacterial composite paper with corn stalk-based carbon spheres immobilized AgNPs.
32487414	3	47	theme	@	440:440	arg1	CS					441:442	AgNPs@CS	435:442	AgNPs@CS	435:442	This study proposed a one-pot hydrothermal strategy to synthesize carbon spheres immobilized silver nanoparticles (AgNPs@CS).
32487414	3	47	theme	@	440:440	arg1	nanoparticles					420:432	carbon spheres immobilized silver nanoparticles	386:432	carbon spheres immobilized silver nanoparticles (AgNPs@CS)	386:443	This study proposed a one-pot hydrothermal strategy to synthesize carbon spheres immobilized silver nanoparticles (AgNPs@CS).
32487414	4	48	theme	silver	474:479	arg1	solution					489:496	silver ammonia solution	474:496	silver ammonia solution	474:496	The synthesis involves with silver ammonia solution as Ag precursor, and corn stalk as green reducing agent and carbon precursor.
32487414	8	49	theme	CS	1097:1098	arg1	paper					1100:1104	CNF/AgNPs@CS paper	1087:1104	CNF/AgNPs@CS paper	1087:1104	In addition, the mechanical and barrier properties of CNF/AgNPs@CS paper were also improved by 29.4% (tensile index), 2.7% (tear index), 7.4% (burst index), 10% (folding endurance), 0.8% (water vapor transmission) and 9.4% (oxygen transmission rate), respectively.
32487414	7	50	theme	anchoring	936:944	arg1	low					983:985	low	983:985	low	983:985	Notably, the accumulative release rate of AgNPs from AgNPs@CS was 10.2% in 9 days, while that from CNF/AgNPs@CS paper was only 6.7% due to the anchoring effect of both CS and CNF, which was low for avoiding the cumulative toxicity problem.
32487414	7	50	theme	anchoring	936:944	arg1	effect					946:951	the anchoring effect	932:951	the anchoring effect	932:951	Notably, the accumulative release rate of AgNPs from AgNPs@CS was 10.2% in 9 days, while that from CNF/AgNPs@CS paper was only 6.7% due to the anchoring effect of both CS and CNF, which was low for avoiding the cumulative toxicity problem.
32487414	8	51	theme	CNF/AgNPs	1087:1095	arg1	paper					1100:1104	CNF/AgNPs@CS paper	1087:1104	CNF/AgNPs@CS paper	1087:1104	In addition, the mechanical and barrier properties of CNF/AgNPs@CS paper were also improved by 29.4% (tensile index), 2.7% (tear index), 7.4% (burst index), 10% (folding endurance), 0.8% (water vapor transmission) and 9.4% (oxygen transmission rate), respectively.
32487414	7	52	theme	CS	961:962	arg1	low					983:985	low	983:985	low	983:985	Notably, the accumulative release rate of AgNPs from AgNPs@CS was 10.2% in 9 days, while that from CNF/AgNPs@CS paper was only 6.7% due to the anchoring effect of both CS and CNF, which was low for avoiding the cumulative toxicity problem.
32487414	7	52	theme	CS	961:962	arg1	effect					946:951	the anchoring effect	932:951	the anchoring effect	932:951	Notably, the accumulative release rate of AgNPs from AgNPs@CS was 10.2% in 9 days, while that from CNF/AgNPs@CS paper was only 6.7% due to the anchoring effect of both CS and CNF, which was low for avoiding the cumulative toxicity problem.
32487414	9	53	theme	antibacterial	1368:1380	arg1	material					1382:1389	a medical antibacterial material	1358:1389	a medical antibacterial material	1358:1389	Therefore, the composite paper has potential application as a medical antibacterial material.
32487414	9	53	theme	antibacterial	1368:1380	arg1	application					1343:1353	potential application	1333:1353	potential application	1333:1353	Therefore, the composite paper has potential application as a medical antibacterial material.
32487414	0	54	with	paper	24:28	arg1	spheres					59:65	corn stalk-based carbon spheres	35:65	corn stalk-based carbon spheres	35:65	Antibacterial composite paper with corn stalk-based carbon spheres immobilized AgNPs.
32487414	0	55	theme	carbon	52:57	arg1	spheres					59:65	corn stalk-based carbon spheres	35:65	corn stalk-based carbon spheres	35:65	Antibacterial composite paper with corn stalk-based carbon spheres immobilized AgNPs.
32487414	4	56	theme	reducing	539:546	arg1	agent					548:552	green reducing agent	533:552	green reducing agent	533:552	The synthesis involves with silver ammonia solution as Ag precursor, and corn stalk as green reducing agent and carbon precursor.
32487414	5	57	theme	antibacterial	655:667	arg1	paper					679:683	the antibacterial composite paper	651:683	the antibacterial composite paper	651:683	Furthermore, AgNPs@CS was anchored by cellulose nanofibers (CNF) to obtain the antibacterial composite paper.
32487414	5	58	theme	cellulose	614:622	arg1	nanofibers					624:633	cellulose nanofibers	614:633	cellulose nanofibers (CNF)	614:639	Furthermore, AgNPs@CS was anchored by cellulose nanofibers (CNF) to obtain the antibacterial composite paper.
32487414	5	58	theme	cellulose	614:622	arg1	CNF					636:638	CNF	636:638	CNF	636:638	Furthermore, AgNPs@CS was anchored by cellulose nanofibers (CNF) to obtain the antibacterial composite paper.
32487414	6	59	theme	antibacterial	737:749	arg1	properties					751:760	superior antibacterial properties	728:760	superior antibacterial properties	728:760	The obtained CNF/AgNPs@CS paper exhibited superior antibacterial properties against E. coli and S. aureus.
32487414	4	60	theme	green	533:537	arg1	agent					548:552	green reducing agent	533:552	green reducing agent	533:552	The synthesis involves with silver ammonia solution as Ag precursor, and corn stalk as green reducing agent and carbon precursor.
32487414	5	61	theme	composite	669:677	arg1	paper					679:683	the antibacterial composite paper	651:683	the antibacterial composite paper	651:683	Furthermore, AgNPs@CS was anchored by cellulose nanofibers (CNF) to obtain the antibacterial composite paper.
32487414	8	62	theme	@	1096:1096	arg1	paper					1100:1104	CNF/AgNPs@CS paper	1087:1104	CNF/AgNPs@CS paper	1087:1104	In addition, the mechanical and barrier properties of CNF/AgNPs@CS paper were also improved by 29.4% (tensile index), 2.7% (tear index), 7.4% (burst index), 10% (folding endurance), 0.8% (water vapor transmission) and 9.4% (oxygen transmission rate), respectively.
32487414	2	63	theme	accumulative	269:280	arg1	toxicity					282:289	accumulative toxicity	269:289	accumulative toxicity	269:289	However, there exist the problems of premature releasing and accumulative toxicity when free AgNPs are applied.
32487414	6	64	theme	superior	728:735	arg1	properties					751:760	superior antibacterial properties	728:760	superior antibacterial properties	728:760	The obtained CNF/AgNPs@CS paper exhibited superior antibacterial properties against E. coli and S. aureus.
32487414	8	65	theme	tear	1157:1160	arg1	%					1154:1154	2.7%	1151:1154	2.7% (tear index)	1151:1167	In addition, the mechanical and barrier properties of CNF/AgNPs@CS paper were also improved by 29.4% (tensile index), 2.7% (tear index), 7.4% (burst index), 10% (folding endurance), 0.8% (water vapor transmission) and 9.4% (oxygen transmission rate), respectively.
32487414	8	65	theme	tear	1157:1160	arg1	index					1162:1166	tear index	1157:1166	tear index	1157:1166	In addition, the mechanical and barrier properties of CNF/AgNPs@CS paper were also improved by 29.4% (tensile index), 2.7% (tear index), 7.4% (burst index), 10% (folding endurance), 0.8% (water vapor transmission) and 9.4% (oxygen transmission rate), respectively.
32487414	8	66	theme	burst	1176:1180	arg1	%					1173:1173	7.4%	1170:1173	7.4% (burst index)	1170:1187	In addition, the mechanical and barrier properties of CNF/AgNPs@CS paper were also improved by 29.4% (tensile index), 2.7% (tear index), 7.4% (burst index), 10% (folding endurance), 0.8% (water vapor transmission) and 9.4% (oxygen transmission rate), respectively.
32487414	8	66	theme	burst	1176:1180	arg1	index					1182:1186	burst index	1176:1186	burst index	1176:1186	In addition, the mechanical and barrier properties of CNF/AgNPs@CS paper were also improved by 29.4% (tensile index), 2.7% (tear index), 7.4% (burst index), 10% (folding endurance), 0.8% (water vapor transmission) and 9.4% (oxygen transmission rate), respectively.
32487414	2	67	theme	toxicity	282:289	arg1	problems					233:240	the problems	229:240	the problems of premature releasing and accumulative toxicity	229:289	However, there exist the problems of premature releasing and accumulative toxicity when free AgNPs are applied.
32487414	7	68	theme	AgNPs	846:850	arg1	CS					852:853	AgNPs@CS	846:853	AgNPs@CS	846:853	Notably, the accumulative release rate of AgNPs from AgNPs@CS was 10.2% in 9 days, while that from CNF/AgNPs@CS paper was only 6.7% due to the anchoring effect of both CS and CNF, which was low for avoiding the cumulative toxicity problem.
32487414	8	69	theme	paper	1100:1104	arg1	properties					1073:1082	the mechanical and barrier properties	1046:1082	properties	1073:1082	In addition, the mechanical and barrier properties of CNF/AgNPs@CS paper were also improved by 29.4% (tensile index), 2.7% (tear index), 7.4% (burst index), 10% (folding endurance), 0.8% (water vapor transmission) and 9.4% (oxygen transmission rate), respectively.
32487414	3	70	theme	one-pot	342:348	arg1	strategy					363:370	a one-pot hydrothermal strategy	340:370	a one-pot hydrothermal strategy to synthesize carbon spheres immobilized silver nanoparticles (AgNPs@CS)	340:443	This study proposed a one-pot hydrothermal strategy to synthesize carbon spheres immobilized silver nanoparticles (AgNPs@CS).
32487414	8	71	theme	transmission	1264:1275	arg1	rate					1277:1280	oxygen transmission rate	1257:1280	oxygen transmission rate	1257:1280	In addition, the mechanical and barrier properties of CNF/AgNPs@CS paper were also improved by 29.4% (tensile index), 2.7% (tear index), 7.4% (burst index), 10% (folding endurance), 0.8% (water vapor transmission) and 9.4% (oxygen transmission rate), respectively.
32487414	8	71	theme	transmission	1264:1275	arg1	%					1254:1254	9.4%	1251:1254	9.4% (oxygen transmission rate)	1251:1281	In addition, the mechanical and barrier properties of CNF/AgNPs@CS paper were also improved by 29.4% (tensile index), 2.7% (tear index), 7.4% (burst index), 10% (folding endurance), 0.8% (water vapor transmission) and 9.4% (oxygen transmission rate), respectively.
32487414	2	72	theme	premature	245:253	arg1	problems					233:240	the problems	229:240	the problems of premature releasing and accumulative toxicity	229:289	However, there exist the problems of premature releasing and accumulative toxicity when free AgNPs are applied.
32487414	6	73	theme	CS	709:710	arg1	paper					712:716	The obtained CNF/AgNPs@CS paper	686:716	The obtained CNF/AgNPs@CS paper	686:716	The obtained CNF/AgNPs@CS paper exhibited superior antibacterial properties against E. coli and S. aureus.
32487414	3	74	theme	immobilized	401:411	arg1	CS					441:442	AgNPs@CS	435:442	AgNPs@CS	435:442	This study proposed a one-pot hydrothermal strategy to synthesize carbon spheres immobilized silver nanoparticles (AgNPs@CS).
32487414	3	74	theme	immobilized	401:411	arg1	nanoparticles					420:432	carbon spheres immobilized silver nanoparticles	386:432	carbon spheres immobilized silver nanoparticles (AgNPs@CS)	386:443	This study proposed a one-pot hydrothermal strategy to synthesize carbon spheres immobilized silver nanoparticles (AgNPs@CS).
32487414	8	75	theme	mechanical	1050:1059	arg1	properties					1073:1082	the mechanical and barrier properties	1046:1082	properties	1073:1082	In addition, the mechanical and barrier properties of CNF/AgNPs@CS paper were also improved by 29.4% (tensile index), 2.7% (tear index), 7.4% (burst index), 10% (folding endurance), 0.8% (water vapor transmission) and 9.4% (oxygen transmission rate), respectively.
33358159	4	0	theme	body	810:813	arg1	weight					815:820	initial body weight	802:820	initial body weight = 90.7 ± 2.15 kg	802:837	Forty-eight calves (initial body weight = 90.7 ± 2.15 kg) were housed in group pens (4 per pen) and fed diets and water for ad libitum intake.
33358159	3	1	theme	%	532:532	arg1	fiber					552:556	64.6% neutral detergent fiber	528:556	64.6% neutral detergent fiber	528:556	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	12	2	theme	fiber	2259:2263	arg1	Digestibility					2196:2208	Digestibility	2196:2208	Digestibility of dry matter, organic matter, neutral detergent fiber, and acid detergent fiber	2196:2289	Digestibility of dry matter, organic matter, neutral detergent fiber, and acid detergent fiber increased from 2 to 3 mo of age, and decreased from 3 to 4 mo of age.
33358159	1	3	theme	forages	197:203	arg1	diet					172:175	a solid diet	164:175	a solid diet of concentrates and forages	164:203	A good transition from a mainly liquid diet to a solid diet of concentrates and forages is important considering the small size and development of the calf's rumen.
33358159	10	4	theme	gain/kg	1760:1766	arg1	kg					1740:1741	0.336, 0.319, and 0.309 kg	1716:1741	0.336, 0.319, and 0.309 kg of average daily gain/kg of dry matter intake	1716:1787	Average daily gain (1.15, 1.12, and 0.95 kg/d), feed efficiency (0.336, 0.319, and 0.309 kg of average daily gain/kg of dry matter intake), and hip width change (4.6, 4.7, and 4.1) decreased linearly with 0, 5, and 10% hay, respectively.
33358159	10	5	theme	matter	1775:1780	arg1	intake					1782:1787	dry matter intake	1771:1787	dry matter intake	1771:1787	Average daily gain (1.15, 1.12, and 0.95 kg/d), feed efficiency (0.336, 0.319, and 0.309 kg of average daily gain/kg of dry matter intake), and hip width change (4.6, 4.7, and 4.1) decreased linearly with 0, 5, and 10% hay, respectively.
33358159	12	6	theme	detergent	2249:2257	arg1	fiber					2259:2263	neutral detergent fiber	2241:2263	neutral detergent fiber	2241:2263	Digestibility of dry matter, organic matter, neutral detergent fiber, and acid detergent fiber increased from 2 to 3 mo of age, and decreased from 3 to 4 mo of age.
33358159	4	7	theme	=	822:822	arg1	weight					815:820	initial body weight	802:820	initial body weight = 90.7 ± 2.15 kg	802:837	Forty-eight calves (initial body weight = 90.7 ± 2.15 kg) were housed in group pens (4 per pen) and fed diets and water for ad libitum intake.
33358159	6	8	theme	Calf	970:973	arg1	weight					980:985	Calf body weight	970:985	Calf body weight	970:985	Calf body weight, hip width, and body condition score (1-5 scale) were measured initially and at 28 and 56 d. Fecal samples were collected from the pen floor with care not to sample bedding material and composited by pen during d 5-9, 26-30, and 47-51 to estimate apparent total-tract digestibility using acid-insoluble ash.
33358159	11	9	dep	5	2070:2070	arg1	to					2067:2068	to	2067:2068	to	2067:2068	Overall estimates of dry matter, organic matter, neutral detergent fiber, acid detergent fiber, and crude protein digestibility changed quadratically with hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay, whereas digestibility of starch, sugar, and fat decreased linearly with increasing hay.
33358159	10	10	theme	width	1799:1803	arg1	change					1805:1810	hip width change	1795:1810	hip width change (4.6, 4.7, and 4.1)	1795:1830	Average daily gain (1.15, 1.12, and 0.95 kg/d), feed efficiency (0.336, 0.319, and 0.309 kg of average daily gain/kg of dry matter intake), and hip width change (4.6, 4.7, and 4.1) decreased linearly with 0, 5, and 10% hay, respectively.
33358159	9	11	theme	Dry	1509:1511	arg1	intake					1520:1525	Dry matter intake	1509:1525	Dry matter intake as a % of body weight	1509:1547	Dry matter intake as a % of body weight changed quadratically with increasing hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay.
33358159	15	12	theme	5	2541:2541	arg1	%					2542:2542	%	2542:2542	%	2542:2542	Feeding 5% chopped hay supported optimal digestion and growth in calves 2 to 4 mo of age, which agrees with previously published research.
33358159	3	13	with	hay	503:505	arg1	starter					588:594	a textured, high-starch starter	564:594	a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats)	564:699	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	10	14	theme	intake	1782:1787	arg1	gain/kg					1760:1766	average daily gain/kg	1746:1766	average daily gain/kg of dry matter intake	1746:1787	Average daily gain (1.15, 1.12, and 0.95 kg/d), feed efficiency (0.336, 0.319, and 0.309 kg of average daily gain/kg of dry matter intake), and hip width change (4.6, 4.7, and 4.1) decreased linearly with 0, 5, and 10% hay, respectively.
33358159	13	15	theme	starch	2378:2383	arg1	Digestibility					2361:2373	Digestibility	2361:2373	Digestibility of starch	2361:2383	Digestibility of starch decreased linearly and digestibility of fat and crude protein increased linearly with age.
33358159	15	16	theme	age	2618:2620	arg1	mo					2612:2613	2 to 4 mo	2605:2613	2 to 4 mo of age, which agrees with previously published research	2605:2669	Feeding 5% chopped hay supported optimal digestion and growth in calves 2 to 4 mo of age, which agrees with previously published research.
33358159	11	17	dep	%	2101:2101	arg1	10					2099:2100	10	2099:2100	10	2099:2100	Overall estimates of dry matter, organic matter, neutral detergent fiber, acid detergent fiber, and crude protein digestibility changed quadratically with hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay, whereas digestibility of starch, sugar, and fat decreased linearly with increasing hay.
33358159	9	18	theme	weight	1542:1547	arg1	%					1532:1532	a %	1530:1532	a % of body weight	1530:1547	Dry matter intake as a % of body weight changed quadratically with increasing hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay.
33358159	9	18	theme	weight	1542:1547	arg1	weight					1542:1547	body weight	1537:1547	body weight	1537:1547	Dry matter intake as a % of body weight changed quadratically with increasing hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay.
33358159	11	19	theme	sugar	2141:2145	arg1	digestibility					2116:2128	digestibility	2116:2128	digestibility of starch, sugar, and fat	2116:2154	Overall estimates of dry matter, organic matter, neutral detergent fiber, acid detergent fiber, and crude protein digestibility changed quadratically with hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay, whereas digestibility of starch, sugar, and fat decreased linearly with increasing hay.
33358159	6	20	theme	bedding	1152:1158	arg1	material					1160:1167	sample bedding material	1145:1167	sample bedding material	1145:1167	Calf body weight, hip width, and body condition score (1-5 scale) were measured initially and at 28 and 56 d. Fecal samples were collected from the pen floor with care not to sample bedding material and composited by pen during d 5-9, 26-30, and 47-51 to estimate apparent total-tract digestibility using acid-insoluble ash.
33358159	3	21	dep	%	487:487	arg1	5					479:479	5	479:479	5	479:479	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	3	21	dep	%	487:487	arg1	10					485:486	10	485:486	10	485:486	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	14	22	theme	age	2528:2530	arg1	mo					2522:2523	4 mo	2520:2523	4 mo of age	2520:2530	Digestive capacity did not appear mature by 4 mo of age.
33358159	10	23	theme	daily	1659:1663	arg1	kg/d					1692:1695	1.15, 1.12, and 0.95 kg/d	1671:1695	1.15, 1.12, and 0.95 kg/d	1671:1695	Average daily gain (1.15, 1.12, and 0.95 kg/d), feed efficiency (0.336, 0.319, and 0.309 kg of average daily gain/kg of dry matter intake), and hip width change (4.6, 4.7, and 4.1) decreased linearly with 0, 5, and 10% hay, respectively.
33358159	10	23	theme	daily	1659:1663	arg1	gain					1665:1668	Average daily gain	1651:1668	Average daily gain (1.15, 1.12, and 0.95 kg/d)	1651:1696	Average daily gain (1.15, 1.12, and 0.95 kg/d), feed efficiency (0.336, 0.319, and 0.309 kg of average daily gain/kg of dry matter intake), and hip width change (4.6, 4.7, and 4.1) decreased linearly with 0, 5, and 10% hay, respectively.
33358159	11	24	theme	starch	2133:2138	arg1	digestibility					2116:2128	digestibility	2116:2128	digestibility of starch, sugar, and fat	2116:2154	Overall estimates of dry matter, organic matter, neutral detergent fiber, acid detergent fiber, and crude protein digestibility changed quadratically with hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay, whereas digestibility of starch, sugar, and fat decreased linearly with increasing hay.
33358159	3	25	theme	crude	603:607	arg1	%					601:601	20.5%	597:601	20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats	597:698	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	3	26	dep	protein	519:525	arg1	fiber					552:556	64.6% neutral detergent fiber	528:556	64.6% neutral detergent fiber	528:556	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	11	27	theme	detergent	1946:1954	arg1	fiber					1956:1960	neutral detergent fiber	1938:1960	neutral detergent fiber	1938:1960	Overall estimates of dry matter, organic matter, neutral detergent fiber, acid detergent fiber, and crude protein digestibility changed quadratically with hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay, whereas digestibility of starch, sugar, and fat decreased linearly with increasing hay.
33358159	0	28	theme	age	112:114	arg1	months					102:107	2 to 4 months	95:107	2 to 4 months of age	95:114	Effect of increasing the amount of hay fed on Holstein calf performance and digestibility from 2 to 4 months of age.
33358159	8	29	theme	grass	1408:1412	arg1	hay					1414:1416	grass hay	1408:1416	grass hay	1408:1416	As grass hay increased, dry matter intake (kg/d) decreased linearly but tended to change quadratically.
33358159	15	30	theme	published	2652:2660	arg1	research					2662:2669	previously published research	2641:2669	previously published research	2641:2669	Feeding 5% chopped hay supported optimal digestion and growth in calves 2 to 4 mo of age, which agrees with previously published research.
33358159	3	31	theme	%	622:622	arg1	starch					624:629	38.4% starch	618:629	38.4% starch	618:629	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	6	32	theme	hip	988:990	arg1	width					992:996	hip width	988:996	hip width	988:996	Calf body weight, hip width, and body condition score (1-5 scale) were measured initially and at 28 and 56 d. Fecal samples were collected from the pen floor with care not to sample bedding material and composited by pen during d 5-9, 26-30, and 47-51 to estimate apparent total-tract digestibility using acid-insoluble ash.
33358159	3	33	theme	grass	497:501	arg1	protein					519:525	6.5% crude protein	508:525	6.5% crude protein	508:525	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	3	33	theme	grass	497:501	arg1	hay					503:505	feeding 0, 5, or 10% chopped grass hay	468:505	feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats)	468:699	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	3	34	theme	detergent	646:654	arg1	fiber					656:660	14.1% neutral detergent fiber	632:660	14.1% neutral detergent fiber	632:660	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	12	35	theme	organic	2225:2231	arg1	matter					2233:2238	organic matter	2225:2238	organic matter	2225:2238	Digestibility of dry matter, organic matter, neutral detergent fiber, and acid detergent fiber increased from 2 to 3 mo of age, and decreased from 3 to 4 mo of age.
33358159	7	36	with	design	1341:1346	arg1	measures					1362:1369	repeated measures	1353:1369	repeated measures	1353:1369	Data were analyzed as a completely randomized design with repeated measures and pen as the experimental unit.
33358159	7	36	with	design	1341:1346	arg1	pen					1375:1377	pen	1375:1377	pen	1375:1377	Data were analyzed as a completely randomized design with repeated measures and pen as the experimental unit.
33358159	11	37	theme	Overall	1889:1895	arg1	estimates					1897:1905	Overall estimates	1889:1905	Overall estimates of dry matter, organic matter, neutral detergent fiber, acid detergent fiber, and crude protein digestibility	1889:2015	Overall estimates of dry matter, organic matter, neutral detergent fiber, acid detergent fiber, and crude protein digestibility changed quadratically with hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay, whereas digestibility of starch, sugar, and fat decreased linearly with increasing hay.
33358159	3	38	theme	protein	663:669	arg1	pellet					671:676	protein pellet	663:676	protein pellet	663:676	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	8	39	theme	dry	1429:1431	arg1	kg/d					1448:1451	kg/d	1448:1451	kg/d	1448:1451	As grass hay increased, dry matter intake (kg/d) decreased linearly but tended to change quadratically.
33358159	8	39	theme	dry	1429:1431	arg1	intake					1440:1445	dry matter intake	1429:1445	dry matter intake (kg/d)	1429:1452	As grass hay increased, dry matter intake (kg/d) decreased linearly but tended to change quadratically.
33358159	3	40	theme	whole	679:683	arg1	corn					685:688	whole corn	679:688	whole corn	679:688	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	9	41	dep	%	1644:1644	arg1	10					1642:1643	10	1642:1643	10	1642:1643	Dry matter intake as a % of body weight changed quadratically with increasing hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay.
33358159	1	42	dep	size	240:243	arg1	the					230:232	the	230:232	the	230:232	A good transition from a mainly liquid diet to a solid diet of concentrates and forages is important considering the small size and development of the calf's rumen.
33358159	8	43	theme	matter	1433:1438	arg1	kg/d					1448:1451	kg/d	1448:1451	kg/d	1448:1451	As grass hay increased, dry matter intake (kg/d) decreased linearly but tended to change quadratically.
33358159	8	43	theme	matter	1433:1438	arg1	intake					1440:1445	dry matter intake	1429:1445	dry matter intake (kg/d)	1429:1452	As grass hay increased, dry matter intake (kg/d) decreased linearly but tended to change quadratically.
33358159	3	44	theme	%	636:636	arg1	fiber					656:660	14.1% neutral detergent fiber	632:660	14.1% neutral detergent fiber	632:660	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	0	45	theme	calf	55:58	arg1	performance					60:70	Holstein calf performance	46:70	Holstein calf performance	46:70	Effect of increasing the amount of hay fed on Holstein calf performance and digestibility from 2 to 4 months of age.
33358159	1	46	theme	rumen	275:279	arg1	development					249:259	development	249:259	development	249:259	A good transition from a mainly liquid diet to a solid diet of concentrates and forages is important considering the small size and development of the calf's rumen.
33358159	1	46	theme	rumen	275:279	arg1	size					240:243	small size	234:243	small size	234:243	A good transition from a mainly liquid diet to a solid diet of concentrates and forages is important considering the small size and development of the calf's rumen.
33358159	1	47	theme	good	119:122	arg1	transition					124:133	A good transition	117:133	A good transition from a mainly liquid diet to a solid diet of concentrates and forages	117:203	A good transition from a mainly liquid diet to a solid diet of concentrates and forages is important considering the small size and development of the calf's rumen.
33358159	1	47	theme	good	119:122	arg1	important					208:216	important	208:216	important	208:216	A good transition from a mainly liquid diet to a solid diet of concentrates and forages is important considering the small size and development of the calf's rumen.
33358159	12	48	theme	neutral	2241:2247	arg1	fiber					2259:2263	neutral detergent fiber	2241:2263	neutral detergent fiber	2241:2263	Digestibility of dry matter, organic matter, neutral detergent fiber, and acid detergent fiber increased from 2 to 3 mo of age, and decreased from 3 to 4 mo of age.
33358159	6	49	theme	acid-insoluble	1275:1288	arg1	ash					1290:1292	acid-insoluble ash	1275:1292	acid-insoluble ash	1275:1292	Calf body weight, hip width, and body condition score (1-5 scale) were measured initially and at 28 and 56 d. Fecal samples were collected from the pen floor with care not to sample bedding material and composited by pen during d 5-9, 26-30, and 47-51 to estimate apparent total-tract digestibility using acid-insoluble ash.
33358159	7	50	theme	randomized	1330:1339	arg1	Data					1295:1298	Data	1295:1298	Data	1295:1298	Data were analyzed as a completely randomized design with repeated measures and pen as the experimental unit.
33358159	7	50	theme	randomized	1330:1339	arg1	design					1341:1346	a completely randomized design	1317:1346	a completely randomized design with repeated measures and pen as the experimental unit	1317:1402	Data were analyzed as a completely randomized design with repeated measures and pen as the experimental unit.
33358159	12	51	theme	matter	2217:2222	arg1	Digestibility					2196:2208	Digestibility	2196:2208	Digestibility of dry matter, organic matter, neutral detergent fiber, and acid detergent fiber	2196:2289	Digestibility of dry matter, organic matter, neutral detergent fiber, and acid detergent fiber increased from 2 to 3 mo of age, and decreased from 3 to 4 mo of age.
33358159	3	52	dep	crude	603:607	arg1	starch					624:629	38.4% starch	618:629	38.4% starch	618:629	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	3	52	dep	crude	603:607	arg1	fiber					656:660	14.1% neutral detergent fiber	632:660	14.1% neutral detergent fiber	632:660	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	3	52	dep	crude	603:607	arg1	protein					609:615	protein	609:615	protein	609:615	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	3	52	dep	crude	603:607	arg1	oats					695:698	oats	695:698	oats	695:698	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	3	52	dep	crude	603:607	arg1	pellet					671:676	protein pellet	663:676	protein pellet	663:676	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	3	52	dep	crude	603:607	arg1	corn					685:688	whole corn	679:688	whole corn	679:688	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	15	53	from	growth	2588:2593	arg1	calves					2598:2603	calves	2598:2603	calves	2598:2603	Feeding 5% chopped hay supported optimal digestion and growth in calves 2 to 4 mo of age, which agrees with previously published research.
33358159	11	54	theme	organic	1922:1928	arg1	matter					1930:1935	organic matter	1922:1935	organic matter	1922:1935	Overall estimates of dry matter, organic matter, neutral detergent fiber, acid detergent fiber, and crude protein digestibility changed quadratically with hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay, whereas digestibility of starch, sugar, and fat decreased linearly with increasing hay.
33358159	4	55	theme	ad	906:907	arg1	intake					917:922	ad libitum intake	906:922	ad libitum intake	906:922	Forty-eight calves (initial body weight = 90.7 ± 2.15 kg) were housed in group pens (4 per pen) and fed diets and water for ad libitum intake.
33358159	15	56	theme	chopped	2544:2550	arg1	hay					2552:2554	Feeding 5% chopped hay	2533:2554	Feeding 5% chopped hay	2533:2554	Feeding 5% chopped hay supported optimal digestion and growth in calves 2 to 4 mo of age, which agrees with previously published research.
33358159	12	57	theme	age	2319:2321	arg1	mo					2313:2314	2 to 3 mo	2306:2314	2 to 3 mo of age	2306:2321	Digestibility of dry matter, organic matter, neutral detergent fiber, and acid detergent fiber increased from 2 to 3 mo of age, and decreased from 3 to 4 mo of age.
33358159	10	58	dep	%	1868:1868	arg1	5					1859:1859	5	1859:1859	5	1859:1859	Average daily gain (1.15, 1.12, and 0.95 kg/d), feed efficiency (0.336, 0.319, and 0.309 kg of average daily gain/kg of dry matter intake), and hip width change (4.6, 4.7, and 4.1) decreased linearly with 0, 5, and 10% hay, respectively.
33358159	10	58	dep	%	1868:1868	arg1	10					1866:1867	10	1866:1867	10	1866:1867	Average daily gain (1.15, 1.12, and 0.95 kg/d), feed efficiency (0.336, 0.319, and 0.309 kg of average daily gain/kg of dry matter intake), and hip width change (4.6, 4.7, and 4.1) decreased linearly with 0, 5, and 10% hay, respectively.
33358159	6	59	theme	apparent	1234:1241	arg1	digestibility					1255:1267	apparent total-tract digestibility	1234:1267	apparent total-tract digestibility using acid-insoluble ash	1234:1292	Calf body weight, hip width, and body condition score (1-5 scale) were measured initially and at 28 and 56 d. Fecal samples were collected from the pen floor with care not to sample bedding material and composited by pen during d 5-9, 26-30, and 47-51 to estimate apparent total-tract digestibility using acid-insoluble ash.
33358159	4	60	theme	initial	802:808	arg1	weight					815:820	initial body weight	802:820	initial body weight = 90.7 ± 2.15 kg	802:837	Forty-eight calves (initial body weight = 90.7 ± 2.15 kg) were housed in group pens (4 per pen) and fed diets and water for ad libitum intake.
33358159	10	61	theme	average	1746:1752	arg1	gain/kg					1760:1766	average daily gain/kg	1746:1766	average daily gain/kg of dry matter intake	1746:1787	Average daily gain (1.15, 1.12, and 0.95 kg/d), feed efficiency (0.336, 0.319, and 0.309 kg of average daily gain/kg of dry matter intake), and hip width change (4.6, 4.7, and 4.1) decreased linearly with 0, 5, and 10% hay, respectively.
33358159	6	62	theme	Fecal	1080:1084	arg1	samples					1086:1092	Fecal samples	1080:1092	Fecal samples	1080:1092	Calf body weight, hip width, and body condition score (1-5 scale) were measured initially and at 28 and 56 d. Fecal samples were collected from the pen floor with care not to sample bedding material and composited by pen during d 5-9, 26-30, and 47-51 to estimate apparent total-tract digestibility using acid-insoluble ash.
33358159	1	63	theme	small	234:238	arg1	size					240:243	small size	234:243	small size	234:243	A good transition from a mainly liquid diet to a solid diet of concentrates and forages is important considering the small size and development of the calf's rumen.
33358159	11	64	theme	protein	1995:2001	arg1	digestibility					2003:2015	crude protein digestibility	1989:2015	crude protein digestibility	1989:2015	Overall estimates of dry matter, organic matter, neutral detergent fiber, acid detergent fiber, and crude protein digestibility changed quadratically with hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay, whereas digestibility of starch, sugar, and fat decreased linearly with increasing hay.
33358159	12	65	dep	4	2348:2348	arg1	to					2345:2346	to	2345:2346	to	2345:2346	Digestibility of dry matter, organic matter, neutral detergent fiber, and acid detergent fiber increased from 2 to 3 mo of age, and decreased from 3 to 4 mo of age.
33358159	3	66	theme	%	511:511	arg1	protein					519:525	6.5% crude protein	508:525	6.5% crude protein	508:525	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	3	66	theme	%	511:511	arg1	hay					503:505	feeding 0, 5, or 10% chopped grass hay	468:505	feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats)	468:699	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	2	67	theme	high-fiber	326:335	arg1	ingredients					337:347	other high-fiber ingredients	320:347	other high-fiber ingredients	320:347	However, the optimal amount of hay or other high-fiber ingredients in the diet of recently weaned calves is not well defined.
33358159	2	68	theme	calves	380:385	arg1	diet					356:359	the diet	352:359	the diet of recently weaned calves	352:385	However, the optimal amount of hay or other high-fiber ingredients in the diet of recently weaned calves is not well defined.
33358159	12	69	theme	acid	2270:2273	arg1	fiber					2285:2289	acid detergent fiber	2270:2289	acid detergent fiber	2270:2289	Digestibility of dry matter, organic matter, neutral detergent fiber, and acid detergent fiber increased from 2 to 3 mo of age, and decreased from 3 to 4 mo of age.
33358159	0	70	theme	hay	35:37	arg1	hay					35:37	hay	35:37	hay	35:37	Effect of increasing the amount of hay fed on Holstein calf performance and digestibility from 2 to 4 months of age.
33358159	0	70	theme	hay	35:37	arg1	amount					25:30	the amount	21:30	the amount of hay	21:37	Effect of increasing the amount of hay fed on Holstein calf performance and digestibility from 2 to 4 months of age.
33358159	11	71	dep	changed	2017:2023	arg1	whereas					2108:2114	whereas	2108:2114	whereas	2108:2114	Overall estimates of dry matter, organic matter, neutral detergent fiber, acid detergent fiber, and crude protein digestibility changed quadratically with hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay, whereas digestibility of starch, sugar, and fat decreased linearly with increasing hay.
33358159	13	72	theme	crude	2433:2437	arg1	protein					2439:2445	crude protein	2433:2445	crude protein	2433:2445	Digestibility of starch decreased linearly and digestibility of fat and crude protein increased linearly with age.
33358159	12	73	theme	age	2356:2358	arg1	mo					2350:2351	3 to 4 mo	2343:2351	3 to 4 mo of age	2343:2358	Digestibility of dry matter, organic matter, neutral detergent fiber, and acid detergent fiber increased from 2 to 3 mo of age, and decreased from 3 to 4 mo of age.
33358159	3	74	theme	64.6	528:531	arg1	%					532:532	%	532:532	%	532:532	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	12	75	theme	fiber	2285:2289	arg1	Digestibility					2196:2208	Digestibility	2196:2208	Digestibility of dry matter, organic matter, neutral detergent fiber, and acid detergent fiber	2196:2289	Digestibility of dry matter, organic matter, neutral detergent fiber, and acid detergent fiber increased from 2 to 3 mo of age, and decreased from 3 to 4 mo of age.
33358159	1	76	theme	solid	166:170	arg1	diet					172:175	a solid diet	164:175	a solid diet of concentrates and forages	164:203	A good transition from a mainly liquid diet to a solid diet of concentrates and forages is important considering the small size and development of the calf's rumen.
33358159	0	77	from	months	102:107	arg1	digestibility					76:88	digestibility	76:88	digestibility	76:88	Effect of increasing the amount of hay fed on Holstein calf performance and digestibility from 2 to 4 months of age.
33358159	0	77	from	months	102:107	arg1	performance					60:70	Holstein calf performance	46:70	Holstein calf performance	46:70	Effect of increasing the amount of hay fed on Holstein calf performance and digestibility from 2 to 4 months of age.
33358159	2	78	theme	optimal	295:301	arg1	hay					313:315	hay	313:315	hay	313:315	However, the optimal amount of hay or other high-fiber ingredients in the diet of recently weaned calves is not well defined.
33358159	2	78	theme	optimal	295:301	arg1	amount					303:308	the optimal amount	291:308	the optimal amount of hay or other high-fiber ingredients in the diet of recently weaned calves	291:385	However, the optimal amount of hay or other high-fiber ingredients in the diet of recently weaned calves is not well defined.
33358159	2	78	theme	optimal	295:301	arg1	ingredients					337:347	other high-fiber ingredients	320:347	other high-fiber ingredients	320:347	However, the optimal amount of hay or other high-fiber ingredients in the diet of recently weaned calves is not well defined.
33358159	14	79	theme	Digestive	2476:2484	arg1	capacity					2486:2493	Digestive capacity	2476:2493	Digestive capacity	2476:2493	Digestive capacity did not appear mature by 4 mo of age.
33358159	15	80	theme	optimal	2566:2572	arg1	digestion					2574:2582	optimal digestion	2566:2582	optimal digestion	2566:2582	Feeding 5% chopped hay supported optimal digestion and growth in calves 2 to 4 mo of age, which agrees with previously published research.
33358159	11	81	theme	crude	1989:1993	arg1	digestibility					2003:2015	crude protein digestibility	1989:2015	crude protein digestibility	1989:2015	Overall estimates of dry matter, organic matter, neutral detergent fiber, acid detergent fiber, and crude protein digestibility changed quadratically with hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay, whereas digestibility of starch, sugar, and fat decreased linearly with increasing hay.
33358159	6	82	theme	pen	1118:1120	arg1	floor					1122:1126	the pen floor	1114:1126	the pen floor with care not to sample bedding material	1114:1167	Calf body weight, hip width, and body condition score (1-5 scale) were measured initially and at 28 and 56 d. Fecal samples were collected from the pen floor with care not to sample bedding material and composited by pen during d 5-9, 26-30, and 47-51 to estimate apparent total-tract digestibility using acid-insoluble ash.
33358159	11	83	theme	matter	1914:1919	arg1	estimates					1897:1905	Overall estimates	1889:1905	Overall estimates of dry matter, organic matter, neutral detergent fiber, acid detergent fiber, and crude protein digestibility	1889:2015	Overall estimates of dry matter, organic matter, neutral detergent fiber, acid detergent fiber, and crude protein digestibility changed quadratically with hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay, whereas digestibility of starch, sugar, and fat decreased linearly with increasing hay.
33358159	6	84	with	floor	1122:1126	arg1	care					1133:1136	care	1133:1136	care	1133:1136	Calf body weight, hip width, and body condition score (1-5 scale) were measured initially and at 28 and 56 d. Fecal samples were collected from the pen floor with care not to sample bedding material and composited by pen during d 5-9, 26-30, and 47-51 to estimate apparent total-tract digestibility using acid-insoluble ash.
33358159	3	85	theme	age	777:779	arg1	mo					771:772	2 and 4 mo	763:772	2 and 4 mo of age	763:779	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	13	86	theme	fat	2425:2427	arg1	digestibility					2408:2420	digestibility	2408:2420	digestibility of fat and crude protein	2408:2445	Digestibility of starch decreased linearly and digestibility of fat and crude protein increased linearly with age.
33358159	9	87	theme	matter	1513:1518	arg1	intake					1520:1525	Dry matter intake	1509:1525	Dry matter intake as a % of body weight	1509:1547	Dry matter intake as a % of body weight changed quadratically with increasing hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay.
33358159	3	88	theme	neutral	534:540	arg1	fiber					552:556	64.6% neutral detergent fiber	528:556	64.6% neutral detergent fiber	528:556	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	6	89	theme	body	975:978	arg1	weight					980:985	Calf body weight	970:985	Calf body weight	970:985	Calf body weight, hip width, and body condition score (1-5 scale) were measured initially and at 28 and 56 d. Fecal samples were collected from the pen floor with care not to sample bedding material and composited by pen during d 5-9, 26-30, and 47-51 to estimate apparent total-tract digestibility using acid-insoluble ash.
33358159	10	90	dep	change	1805:1810	arg1	4.7					1818:1820	4.7	1818:1820	4.7	1818:1820	Average daily gain (1.15, 1.12, and 0.95 kg/d), feed efficiency (0.336, 0.319, and 0.309 kg of average daily gain/kg of dry matter intake), and hip width change (4.6, 4.7, and 4.1) decreased linearly with 0, 5, and 10% hay, respectively.
33358159	10	90	dep	change	1805:1810	arg1	4.1					1827:1829	4.1	1827:1829	4.1	1827:1829	Average daily gain (1.15, 1.12, and 0.95 kg/d), feed efficiency (0.336, 0.319, and 0.309 kg of average daily gain/kg of dry matter intake), and hip width change (4.6, 4.7, and 4.1) decreased linearly with 0, 5, and 10% hay, respectively.
33358159	15	91	theme	%	2542:2542	arg1	hay					2552:2554	Feeding 5% chopped hay	2533:2554	Feeding 5% chopped hay	2533:2554	Feeding 5% chopped hay supported optimal digestion and growth in calves 2 to 4 mo of age, which agrees with previously published research.
33358159	10	92	theme	dry	1771:1773	arg1	intake					1782:1787	dry matter intake	1771:1787	dry matter intake	1771:1787	Average daily gain (1.15, 1.12, and 0.95 kg/d), feed efficiency (0.336, 0.319, and 0.309 kg of average daily gain/kg of dry matter intake), and hip width change (4.6, 4.7, and 4.1) decreased linearly with 0, 5, and 10% hay, respectively.
33358159	3	93	theme	textured	566:573	arg1	starter					588:594	a textured, high-starch starter	564:594	a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats)	564:699	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	15	94	theme	Feeding	2533:2539	arg1	hay					2552:2554	Feeding 5% chopped hay	2533:2554	Feeding 5% chopped hay	2533:2554	Feeding 5% chopped hay supported optimal digestion and growth in calves 2 to 4 mo of age, which agrees with previously published research.
33358159	10	95	theme	hip	1795:1797	arg1	change					1805:1810	hip width change	1795:1810	hip width change (4.6, 4.7, and 4.1)	1795:1830	Average daily gain (1.15, 1.12, and 0.95 kg/d), feed efficiency (0.336, 0.319, and 0.309 kg of average daily gain/kg of dry matter intake), and hip width change (4.6, 4.7, and 4.1) decreased linearly with 0, 5, and 10% hay, respectively.
33358159	15	96	dep	4	2610:2610	arg1	to					2607:2608	to	2607:2608	to	2607:2608	Feeding 5% chopped hay supported optimal digestion and growth in calves 2 to 4 mo of age, which agrees with previously published research.
33358159	4	97	dep	calves	794:799	arg1	weight					815:820	initial body weight	802:820	initial body weight = 90.7 ± 2.15 kg	802:837	Forty-eight calves (initial body weight = 90.7 ± 2.15 kg) were housed in group pens (4 per pen) and fed diets and water for ad libitum intake.
33358159	3	98	theme	feeding	468:474	arg1	protein					519:525	6.5% crude protein	508:525	6.5% crude protein	508:525	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	3	98	theme	feeding	468:474	arg1	hay					503:505	feeding 0, 5, or 10% chopped grass hay	468:505	feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats)	468:699	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	3	99	dep	calves	748:753	arg1	steer					742:746	steer	742:746	steer	742:746	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	10	100	theme	Average	1651:1657	arg1	kg/d					1692:1695	1.15, 1.12, and 0.95 kg/d	1671:1695	1.15, 1.12, and 0.95 kg/d	1671:1695	Average daily gain (1.15, 1.12, and 0.95 kg/d), feed efficiency (0.336, 0.319, and 0.309 kg of average daily gain/kg of dry matter intake), and hip width change (4.6, 4.7, and 4.1) decreased linearly with 0, 5, and 10% hay, respectively.
33358159	10	100	theme	Average	1651:1657	arg1	gain					1665:1668	Average daily gain	1651:1668	Average daily gain (1.15, 1.12, and 0.95 kg/d)	1651:1696	Average daily gain (1.15, 1.12, and 0.95 kg/d), feed efficiency (0.336, 0.319, and 0.309 kg of average daily gain/kg of dry matter intake), and hip width change (4.6, 4.7, and 4.1) decreased linearly with 0, 5, and 10% hay, respectively.
33358159	3	101	dep	starter	588:594	arg1	%					601:601	20.5%	597:601	20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats	597:698	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	11	102	dep	10	2099:2100	arg1	to					2096:2097	to	2096:2097	to	2096:2097	Overall estimates of dry matter, organic matter, neutral detergent fiber, acid detergent fiber, and crude protein digestibility changed quadratically with hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay, whereas digestibility of starch, sugar, and fat decreased linearly with increasing hay.
33358159	9	103	theme	body	1537:1540	arg1	weight					1542:1547	body weight	1537:1547	body weight	1537:1547	Dry matter intake as a % of body weight changed quadratically with increasing hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay.
33358159	1	104	theme	liquid	149:154	arg1	diet					156:159	a mainly liquid diet	140:159	a mainly liquid diet	140:159	A good transition from a mainly liquid diet to a solid diet of concentrates and forages is important considering the small size and development of the calf's rumen.
33358159	11	105	theme	fiber	1956:1960	arg1	estimates					1897:1905	Overall estimates	1889:1905	Overall estimates of dry matter, organic matter, neutral detergent fiber, acid detergent fiber, and crude protein digestibility	1889:2015	Overall estimates of dry matter, organic matter, neutral detergent fiber, acid detergent fiber, and crude protein digestibility changed quadratically with hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay, whereas digestibility of starch, sugar, and fat decreased linearly with increasing hay.
33358159	11	106	dep	hay	2103:2105	arg1	%					2101:2101	%	2101:2101	%	2101:2101	Overall estimates of dry matter, organic matter, neutral detergent fiber, acid detergent fiber, and crude protein digestibility changed quadratically with hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay, whereas digestibility of starch, sugar, and fat decreased linearly with increasing hay.
33358159	4	107	theme	group	855:859	arg1	pens					861:864	group pens	855:864	group pens (4 per pen)	855:876	Forty-eight calves (initial body weight = 90.7 ± 2.15 kg) were housed in group pens (4 per pen) and fed diets and water for ad libitum intake.
33358159	6	108	theme	condition	1008:1016	arg1	score					1018:1022	body condition score	1003:1022	body condition score (1-5 scale)	1003:1034	Calf body weight, hip width, and body condition score (1-5 scale) were measured initially and at 28 and 56 d. Fecal samples were collected from the pen floor with care not to sample bedding material and composited by pen during d 5-9, 26-30, and 47-51 to estimate apparent total-tract digestibility using acid-insoluble ash.
33358159	6	108	theme	condition	1008:1016	arg1	scale					1029:1033	1-5 scale	1025:1033	1-5 scale	1025:1033	Calf body weight, hip width, and body condition score (1-5 scale) were measured initially and at 28 and 56 d. Fecal samples were collected from the pen floor with care not to sample bedding material and composited by pen during d 5-9, 26-30, and 47-51 to estimate apparent total-tract digestibility using acid-insoluble ash.
33358159	6	109	theme	sample	1145:1150	arg1	material					1160:1167	sample bedding material	1145:1167	sample bedding material	1145:1167	Calf body weight, hip width, and body condition score (1-5 scale) were measured initially and at 28 and 56 d. Fecal samples were collected from the pen floor with care not to sample bedding material and composited by pen during d 5-9, 26-30, and 47-51 to estimate apparent total-tract digestibility using acid-insoluble ash.
33358159	5	110	dep	Feed	925:928	arg1	offered					930:936	offered	930:936	offered	930:936	Feed offered and refused was measured daily.
33358159	5	110	dep	Feed	925:928	arg1	refused					942:948	refused	942:948	refused	942:948	Feed offered and refused was measured daily.
33358159	3	111	from	performance	704:714	arg1	calves					748:753	Holstein steer calves	733:753	Holstein steer calves between 2 and 4 mo of age	733:779	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	15	112	from	digestion	2574:2582	arg1	calves					2598:2603	calves	2598:2603	calves	2598:2603	Feeding 5% chopped hay supported optimal digestion and growth in calves 2 to 4 mo of age, which agrees with previously published research.
33358159	11	113	theme	fiber	1978:1982	arg1	estimates					1897:1905	Overall estimates	1889:1905	Overall estimates of dry matter, organic matter, neutral detergent fiber, acid detergent fiber, and crude protein digestibility	1889:2015	Overall estimates of dry matter, organic matter, neutral detergent fiber, acid detergent fiber, and crude protein digestibility changed quadratically with hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay, whereas digestibility of starch, sugar, and fat decreased linearly with increasing hay.
33358159	3	114	from	digestion	720:728	arg1	calves					748:753	Holstein steer calves	733:753	Holstein steer calves between 2 and 4 mo of age	733:779	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	10	115	theme	feed	1699:1702	arg1	efficiency					1704:1713	feed efficiency	1699:1713	feed efficiency (0.336, 0.319, and 0.309 kg of average daily gain/kg of dry matter intake)	1699:1788	Average daily gain (1.15, 1.12, and 0.95 kg/d), feed efficiency (0.336, 0.319, and 0.309 kg of average daily gain/kg of dry matter intake), and hip width change (4.6, 4.7, and 4.1) decreased linearly with 0, 5, and 10% hay, respectively.
33358159	3	116	from	effects	457:463	arg1	performance					704:714	performance	704:714	performance	704:714	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	3	116	from	effects	457:463	arg1	digestion					720:728	digestion	720:728	digestion	720:728	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	3	117	theme	chopped	489:495	arg1	protein					519:525	6.5% crude protein	508:525	6.5% crude protein	508:525	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	3	117	theme	chopped	489:495	arg1	hay					503:505	feeding 0, 5, or 10% chopped grass hay	468:505	feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats)	468:699	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	1	118	from	diet	156:159	arg1	transition					124:133	A good transition	117:133	A good transition from a mainly liquid diet to a solid diet of concentrates and forages	117:203	A good transition from a mainly liquid diet to a solid diet of concentrates and forages is important considering the small size and development of the calf's rumen.
33358159	1	118	from	diet	156:159	arg1	important					208:216	important	208:216	important	208:216	A good transition from a mainly liquid diet to a solid diet of concentrates and forages is important considering the small size and development of the calf's rumen.
33358159	7	119	theme	experimental	1386:1397	arg1	unit					1399:1402	the experimental unit	1382:1402	the experimental unit	1382:1402	Data were analyzed as a completely randomized design with repeated measures and pen as the experimental unit.
33358159	9	120	dep	hay	1646:1648	arg1	%					1644:1644	%	1644:1644	%	1644:1644	Dry matter intake as a % of body weight changed quadratically with increasing hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay.
33358159	11	121	theme	acid	1963:1966	arg1	fiber					1978:1982	acid detergent fiber	1963:1982	acid detergent fiber	1963:1982	Overall estimates of dry matter, organic matter, neutral detergent fiber, acid detergent fiber, and crude protein digestibility changed quadratically with hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay, whereas digestibility of starch, sugar, and fat decreased linearly with increasing hay.
33358159	0	122	theme	Holstein	46:53	arg1	performance					60:70	Holstein calf performance	46:70	Holstein calf performance	46:70	Effect of increasing the amount of hay fed on Holstein calf performance and digestibility from 2 to 4 months of age.
33358159	9	123	dep	10	1642:1643	arg1	to					1639:1640	to	1639:1640	to	1639:1640	Dry matter intake as a % of body weight changed quadratically with increasing hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay.
33358159	11	124	theme	dry	1910:1912	arg1	matter					1914:1919	dry matter	1910:1919	dry matter	1910:1919	Overall estimates of dry matter, organic matter, neutral detergent fiber, acid detergent fiber, and crude protein digestibility changed quadratically with hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay, whereas digestibility of starch, sugar, and fat decreased linearly with increasing hay.
33358159	12	125	dep	3	2311:2311	arg1	to					2308:2309	to	2308:2309	to	2308:2309	Digestibility of dry matter, organic matter, neutral detergent fiber, and acid detergent fiber increased from 2 to 3 mo of age, and decreased from 3 to 4 mo of age.
33358159	4	126	theme	fed	882:884	arg1	diets					886:890	fed diets	882:890	fed diets	882:890	Forty-eight calves (initial body weight = 90.7 ± 2.15 kg) were housed in group pens (4 per pen) and fed diets and water for ad libitum intake.
33358159	3	127	dep	hay	503:505	arg1	%					487:487	%	487:487	%	487:487	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	3	128	theme	38.4	618:621	arg1	%					622:622	%	622:622	%	622:622	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	4	129	dep	pens	861:864	arg1	4					867:867	4	867:867	4	867:867	Forty-eight calves (initial body weight = 90.7 ± 2.15 kg) were housed in group pens (4 per pen) and fed diets and water for ad libitum intake.
33358159	0	130	dep	4	100:100	arg1	to					97:98	to	97:98	to	97:98	Effect of increasing the amount of hay fed on Holstein calf performance and digestibility from 2 to 4 months of age.
33358159	12	131	theme	matter	2233:2238	arg1	Digestibility					2196:2208	Digestibility	2196:2208	Digestibility of dry matter, organic matter, neutral detergent fiber, and acid detergent fiber	2196:2289	Digestibility of dry matter, organic matter, neutral detergent fiber, and acid detergent fiber increased from 2 to 3 mo of age, and decreased from 3 to 4 mo of age.
33358159	3	132	theme	neutral	638:644	arg1	fiber					656:660	14.1% neutral detergent fiber	632:660	14.1% neutral detergent fiber	632:660	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	11	133	theme	detergent	1968:1976	arg1	fiber					1978:1982	acid detergent fiber	1963:1982	acid detergent fiber	1963:1982	Overall estimates of dry matter, organic matter, neutral detergent fiber, acid detergent fiber, and crude protein digestibility changed quadratically with hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay, whereas digestibility of starch, sugar, and fat decreased linearly with increasing hay.
33358159	4	134	dep	=	822:822	arg1	kg					836:837	90.7 ± 2.15 kg	824:837	90.7 ± 2.15 kg	824:837	Forty-eight calves (initial body weight = 90.7 ± 2.15 kg) were housed in group pens (4 per pen) and fed diets and water for ad libitum intake.
33358159	7	135	theme	repeated	1353:1360	arg1	measures					1362:1369	repeated measures	1353:1369	repeated measures	1353:1369	Data were analyzed as a completely randomized design with repeated measures and pen as the experimental unit.
33358159	11	136	theme	matter	1930:1935	arg1	estimates					1897:1905	Overall estimates	1889:1905	Overall estimates of dry matter, organic matter, neutral detergent fiber, acid detergent fiber, and crude protein digestibility	1889:2015	Overall estimates of dry matter, organic matter, neutral detergent fiber, acid detergent fiber, and crude protein digestibility changed quadratically with hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay, whereas digestibility of starch, sugar, and fat decreased linearly with increasing hay.
33358159	3	137	theme	14.1	632:635	arg1	%					636:636	%	636:636	%	636:636	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	12	138	theme	dry	2213:2215	arg1	matter					2217:2222	dry matter	2213:2222	dry matter	2213:2222	Digestibility of dry matter, organic matter, neutral detergent fiber, and acid detergent fiber increased from 2 to 3 mo of age, and decreased from 3 to 4 mo of age.
33358159	4	139	theme	libitum	909:915	arg1	intake					917:922	ad libitum intake	906:922	ad libitum intake	906:922	Forty-eight calves (initial body weight = 90.7 ± 2.15 kg) were housed in group pens (4 per pen) and fed diets and water for ad libitum intake.
33358159	11	140	theme	digestibility	2003:2015	arg1	estimates					1897:1905	Overall estimates	1889:1905	Overall estimates of dry matter, organic matter, neutral detergent fiber, acid detergent fiber, and crude protein digestibility	1889:2015	Overall estimates of dry matter, organic matter, neutral detergent fiber, acid detergent fiber, and crude protein digestibility changed quadratically with hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay, whereas digestibility of starch, sugar, and fat decreased linearly with increasing hay.
33358159	2	141	theme	hay	313:315	arg1	hay					313:315	hay	313:315	hay	313:315	However, the optimal amount of hay or other high-fiber ingredients in the diet of recently weaned calves is not well defined.
33358159	2	141	theme	hay	313:315	arg1	amount					303:308	the optimal amount	291:308	the optimal amount of hay or other high-fiber ingredients in the diet of recently weaned calves	291:385	However, the optimal amount of hay or other high-fiber ingredients in the diet of recently weaned calves is not well defined.
33358159	2	141	theme	hay	313:315	arg1	ingredients					337:347	other high-fiber ingredients	320:347	other high-fiber ingredients	320:347	However, the optimal amount of hay or other high-fiber ingredients in the diet of recently weaned calves is not well defined.
33358159	10	142	dep	hay	1870:1872	arg1	%					1868:1868	%	1868:1868	%	1868:1868	Average daily gain (1.15, 1.12, and 0.95 kg/d), feed efficiency (0.336, 0.319, and 0.309 kg of average daily gain/kg of dry matter intake), and hip width change (4.6, 4.7, and 4.1) decreased linearly with 0, 5, and 10% hay, respectively.
33358159	6	143	theme	total-tract	1243:1253	arg1	digestibility					1255:1267	apparent total-tract digestibility	1234:1267	apparent total-tract digestibility using acid-insoluble ash	1234:1292	Calf body weight, hip width, and body condition score (1-5 scale) were measured initially and at 28 and 56 d. Fecal samples were collected from the pen floor with care not to sample bedding material and composited by pen during d 5-9, 26-30, and 47-51 to estimate apparent total-tract digestibility using acid-insoluble ash.
33358159	10	144	theme	daily	1754:1758	arg1	gain/kg					1760:1766	average daily gain/kg	1746:1766	average daily gain/kg of dry matter intake	1746:1787	Average daily gain (1.15, 1.12, and 0.95 kg/d), feed efficiency (0.336, 0.319, and 0.309 kg of average daily gain/kg of dry matter intake), and hip width change (4.6, 4.7, and 4.1) decreased linearly with 0, 5, and 10% hay, respectively.
33358159	2	145	theme	ingredients	337:347	arg1	hay					313:315	hay	313:315	hay	313:315	However, the optimal amount of hay or other high-fiber ingredients in the diet of recently weaned calves is not well defined.
33358159	2	145	theme	ingredients	337:347	arg1	amount					303:308	the optimal amount	291:308	the optimal amount of hay or other high-fiber ingredients in the diet of recently weaned calves	291:385	However, the optimal amount of hay or other high-fiber ingredients in the diet of recently weaned calves is not well defined.
33358159	2	145	theme	ingredients	337:347	arg1	ingredients					337:347	other high-fiber ingredients	320:347	other high-fiber ingredients	320:347	However, the optimal amount of hay or other high-fiber ingredients in the diet of recently weaned calves is not well defined.
33358159	15	146	dep	digestion	2574:2582	arg1	mo					2612:2613	2 to 4 mo	2605:2613	2 to 4 mo of age, which agrees with previously published research	2605:2669	Feeding 5% chopped hay supported optimal digestion and growth in calves 2 to 4 mo of age, which agrees with previously published research.
33358159	10	147	dep	efficiency	1704:1713	arg1	kg					1740:1741	0.336, 0.319, and 0.309 kg	1716:1741	0.336, 0.319, and 0.309 kg of average daily gain/kg of dry matter intake	1716:1787	Average daily gain (1.15, 1.12, and 0.95 kg/d), feed efficiency (0.336, 0.319, and 0.309 kg of average daily gain/kg of dry matter intake), and hip width change (4.6, 4.7, and 4.1) decreased linearly with 0, 5, and 10% hay, respectively.
33358159	3	148	theme	hay	503:505	arg1	effects					457:463	the effects	453:463	the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age	453:779	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	2	149	theme	other	320:324	arg1	ingredients					337:347	other high-fiber ingredients	320:347	other high-fiber ingredients	320:347	However, the optimal amount of hay or other high-fiber ingredients in the diet of recently weaned calves is not well defined.
33358159	2	150	from	amount	303:308	arg1	diet					356:359	the diet	352:359	the diet of recently weaned calves	352:385	However, the optimal amount of hay or other high-fiber ingredients in the diet of recently weaned calves is not well defined.
33358159	2	151	theme	weaned	373:378	arg1	calves					380:385	recently weaned calves	364:385	recently weaned calves	364:385	However, the optimal amount of hay or other high-fiber ingredients in the diet of recently weaned calves is not well defined.
33358159	11	152	theme	fat	2152:2154	arg1	digestibility					2116:2128	digestibility	2116:2128	digestibility of starch, sugar, and fat	2116:2154	Overall estimates of dry matter, organic matter, neutral detergent fiber, acid detergent fiber, and crude protein digestibility changed quadratically with hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay, whereas digestibility of starch, sugar, and fat decreased linearly with increasing hay.
33358159	11	153	theme	neutral	1938:1944	arg1	fiber					1956:1960	neutral detergent fiber	1938:1960	neutral detergent fiber	1938:1960	Overall estimates of dry matter, organic matter, neutral detergent fiber, acid detergent fiber, and crude protein digestibility changed quadratically with hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay, whereas digestibility of starch, sugar, and fat decreased linearly with increasing hay.
33358159	3	154	dep	textured	566:573	arg1	high-starch					576:586	high-starch	576:586	high-starch	576:586	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	3	155	theme	Holstein	733:740	arg1	calves					748:753	Holstein steer calves	733:753	Holstein steer calves between 2 and 4 mo of age	733:779	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	13	156	theme	protein	2439:2445	arg1	digestibility					2408:2420	digestibility	2408:2420	digestibility of fat and crude protein	2408:2445	Digestibility of starch decreased linearly and digestibility of fat and crude protein increased linearly with age.
33358159	4	157	theme	±	829:829	arg1	kg					836:837	90.7 ± 2.15 kg	824:837	90.7 ± 2.15 kg	824:837	Forty-eight calves (initial body weight = 90.7 ± 2.15 kg) were housed in group pens (4 per pen) and fed diets and water for ad libitum intake.
33358159	6	158	theme	body	1003:1006	arg1	score					1018:1022	body condition score	1003:1022	body condition score (1-5 scale)	1003:1034	Calf body weight, hip width, and body condition score (1-5 scale) were measured initially and at 28 and 56 d. Fecal samples were collected from the pen floor with care not to sample bedding material and composited by pen during d 5-9, 26-30, and 47-51 to estimate apparent total-tract digestibility using acid-insoluble ash.
33358159	6	158	theme	body	1003:1006	arg1	scale					1029:1033	1-5 scale	1025:1033	1-5 scale	1025:1033	Calf body weight, hip width, and body condition score (1-5 scale) were measured initially and at 28 and 56 d. Fecal samples were collected from the pen floor with care not to sample bedding material and composited by pen during d 5-9, 26-30, and 47-51 to estimate apparent total-tract digestibility using acid-insoluble ash.
33358159	3	159	theme	crude	513:517	arg1	protein					519:525	6.5% crude protein	508:525	6.5% crude protein	508:525	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	3	159	theme	crude	513:517	arg1	hay					503:505	feeding 0, 5, or 10% chopped grass hay	468:505	feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats)	468:699	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	12	160	theme	detergent	2275:2283	arg1	fiber					2285:2289	acid detergent fiber	2270:2289	acid detergent fiber	2270:2289	Digestibility of dry matter, organic matter, neutral detergent fiber, and acid detergent fiber increased from 2 to 3 mo of age, and decreased from 3 to 4 mo of age.
33358159	9	161	dep	5	1613:1613	arg1	to					1610:1611	to	1610:1611	to	1610:1611	Dry matter intake as a % of body weight changed quadratically with increasing hay, increasing from 0 to 5%, and decreasing from 5 to 10% hay.
33358159	3	162	theme	detergent	542:550	arg1	fiber					552:556	64.6% neutral detergent fiber	528:556	64.6% neutral detergent fiber	528:556	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	3	163	theme	trial	430:434	arg1	objective					412:420	The objective	408:420	The objective of this trial	408:434	The objective of this trial was to determine the effects of feeding 0, 5, or 10% chopped grass hay (6.5% crude protein, 64.6% neutral detergent fiber) with a textured, high-starch starter (20.5% crude protein, 38.4% starch, 14.1% neutral detergent fiber; protein pellet, whole corn, and oats) on performance and digestion in Holstein steer calves between 2 and 4 mo of age.
33358159	1	164	theme	concentrates	180:191	arg1	diet					172:175	a solid diet	164:175	a solid diet of concentrates and forages	164:203	A good transition from a mainly liquid diet to a solid diet of concentrates and forages is important considering the small size and development of the calf's rumen.
32701290	6	0	theme	pristine	966:973	arg1	h-1					997:999	45 L m-2 h-1	988:999	45 L m-2 h-1	988:999	Improved salt rejection performances were observed for the grafted PA membranes at the expense of negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1) compared with the pristine PA membrane (45 L m-2 h-1).
32701290	6	0	theme	pristine	966:973	arg1	membrane					978:985	the pristine PA membrane	962:985	the pristine PA membrane (45 L m-2 h-1)	962:1000	Improved salt rejection performances were observed for the grafted PA membranes at the expense of negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1) compared with the pristine PA membrane (45 L m-2 h-1).
32701290	2	1	theme	membrane	520:527	arg1	surface					529:535	TFC-RO membrane surface	513:535	TFC-RO membrane surface	513:535	In this work, alginate dialdehyde (ADA) was used as a green platform to graft chlorhexidine (CH), a nonoxidizing chemical, on TFC-RO membrane surface.
32701290	10	2	theme	green	1577:1581	arg1	route					1595:1599	a new robust and green bioinspired route	1560:1599	a new robust and green bioinspired route for practical application in RO membrane fouling control	1560:1656	Hence, these results pave the way for a new robust and green bioinspired route for practical application in RO membrane fouling control.
32701290	6	3	theme	rejection	794:802	arg1	performances					804:815	Improved salt rejection performances	780:815	Improved salt rejection performances	780:815	Improved salt rejection performances were observed for the grafted PA membranes at the expense of negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1) compared with the pristine PA membrane (45 L m-2 h-1).
32701290	10	4	theme	robust	1566:1571	arg1	route					1595:1599	a new robust and green bioinspired route	1560:1599	a new robust and green bioinspired route for practical application in RO membrane fouling control	1560:1656	Hence, these results pave the way for a new robust and green bioinspired route for practical application in RO membrane fouling control.
32701290	6	5	theme	Improved	780:787	arg1	performances					804:815	Improved salt rejection performances	780:815	Improved salt rejection performances	780:815	Improved salt rejection performances were observed for the grafted PA membranes at the expense of negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1) compared with the pristine PA membrane (45 L m-2 h-1).
32701290	7	6	contain	had	1031:1033	arg1	membranes					1021:1029	All the CH-ADA-PA membranes	1003:1029	All the CH-ADA-PA membranes	1003:1029	All the CH-ADA-PA membranes had excellent antibacterial activity against E. coli along with a highly superior resistance to the formation of biofilms.
32701290	7	6	contain	had	1031:1033	arg2	activity					1059:1066	excellent antibacterial activity	1035:1066	excellent antibacterial activity against E. coli	1035:1082	All the CH-ADA-PA membranes had excellent antibacterial activity against E. coli along with a highly superior resistance to the formation of biofilms.
32701290	10	7	theme	practical	1605:1613	arg1	application					1615:1625	practical application	1605:1625	practical application in RO membrane fouling control	1605:1656	Hence, these results pave the way for a new robust and green bioinspired route for practical application in RO membrane fouling control.
32701290	1	8	theme	robust	176:181	arg1	platform					200:207	a new robust and green facile platform	170:207	a new robust and green facile platform for nonoxidizing chemical grafting to simultaneously improve antifouling and antibacterial properties of thin film composite (TFC) polyamide (PA) reverse osmosis (RO) membranes	170:384	We report a new robust and green facile platform for nonoxidizing chemical grafting to simultaneously improve antifouling and antibacterial properties of thin film composite (TFC) polyamide (PA) reverse osmosis (RO) membranes.
32701290	5	9	theme	two-step	735:742	arg1	reaction					756:763	A simple two-step Schiff base reaction	726:763	A simple two-step Schiff base reaction	726:763	A simple two-step Schiff base reaction was performed.
32701290	0	10	theme	In	132:133	arg1	Control					151:157	In Situ Biofouling Control	132:157	In Situ Biofouling Control	132:157	Surface Grafting of Reverse Osmosis Membrane with Chlorhexidine Using Biopolymer Alginate Dialdehyde as a Facile Green Platform for In Situ Biofouling Control.
32701290	10	11	theme	membrane	1633:1640	arg1	control					1650:1656	RO membrane fouling control	1630:1656	RO membrane fouling control	1630:1656	Hence, these results pave the way for a new robust and green bioinspired route for practical application in RO membrane fouling control.
32701290	5	12	theme	base	751:754	arg1	reaction					756:763	A simple two-step Schiff base reaction	726:763	A simple two-step Schiff base reaction	726:763	A simple two-step Schiff base reaction was performed.
32701290	0	13	theme	Biofouling	140:149	arg1	Control					151:157	In Situ Biofouling Control	132:157	In Situ Biofouling Control	132:157	Surface Grafting of Reverse Osmosis Membrane with Chlorhexidine Using Biopolymer Alginate Dialdehyde as a Facile Green Platform for In Situ Biofouling Control.
32701290	1	14	dep	antifouling	270:280	arg1	membranes					376:384	composite (TFC) polyamide (PA) reverse osmosis (RO) membranes	324:384	composite (TFC) polyamide (PA) reverse osmosis (RO) membranes	324:384	We report a new robust and green facile platform for nonoxidizing chemical grafting to simultaneously improve antifouling and antibacterial properties of thin film composite (TFC) polyamide (PA) reverse osmosis (RO) membranes.
32701290	6	15	theme	grafted	839:845	arg1	membranes					850:858	the grafted PA membranes	835:858	the grafted PA membranes at the expense of negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1)	835:946	Improved salt rejection performances were observed for the grafted PA membranes at the expense of negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1) compared with the pristine PA membrane (45 L m-2 h-1).
32701290	8	16	theme	typical	1303:1309	arg1	foulants					1311:1318	typical foulants	1303:1318	typical foulants for the grafted PA membranes	1303:1347	Organic fouling behaviors with a protein (bovine serum albumin, BSA) and a surfactant (dodecyl trimethylammonium bromide, DTAB) were investigated as typical foulants for the grafted PA membranes.
32701290	8	16	theme	typical	1303:1309	arg1	behaviors					1170:1178	Organic fouling behaviors	1154:1178	Organic fouling behaviors with a protein (bovine serum albumin, BSA) and a surfactant (dodecyl trimethylammonium bromide, DTAB)	1154:1280	Organic fouling behaviors with a protein (bovine serum albumin, BSA) and a surfactant (dodecyl trimethylammonium bromide, DTAB) were investigated as typical foulants for the grafted PA membranes.
32701290	8	17	theme	fouling	1162:1168	arg1	foulants					1311:1318	typical foulants	1303:1318	typical foulants for the grafted PA membranes	1303:1347	Organic fouling behaviors with a protein (bovine serum albumin, BSA) and a surfactant (dodecyl trimethylammonium bromide, DTAB) were investigated as typical foulants for the grafted PA membranes.
32701290	8	17	theme	fouling	1162:1168	arg1	behaviors					1170:1178	Organic fouling behaviors	1154:1178	Organic fouling behaviors with a protein (bovine serum albumin, BSA) and a surfactant (dodecyl trimethylammonium bromide, DTAB)	1154:1280	Organic fouling behaviors with a protein (bovine serum albumin, BSA) and a surfactant (dodecyl trimethylammonium bromide, DTAB) were investigated as typical foulants for the grafted PA membranes.
32701290	4	18	theme	modified	604:611	arg1	surfaces					622:629	The modified membrane surfaces	600:629	The modified membrane surfaces	600:629	The modified membrane surfaces were characterized using XPS, FT-IR, AFM, SEM-EDS, contact angle, and zeta potential analysis.
32701290	1	19	theme	TFC	335:337	arg1	membranes					376:384	composite (TFC) polyamide (PA) reverse osmosis (RO) membranes	324:384	composite (TFC) polyamide (PA) reverse osmosis (RO) membranes	324:384	We report a new robust and green facile platform for nonoxidizing chemical grafting to simultaneously improve antifouling and antibacterial properties of thin film composite (TFC) polyamide (PA) reverse osmosis (RO) membranes.
32701290	3	20	theme	synergistic	540:550	arg1	effect					552:557	A synergistic effect	538:557	A synergistic effect due to ADA and CH grafting	538:584	A synergistic effect due to ADA and CH grafting was revealed.
32701290	0	21	dep	In	132:133	arg1	Situ					135:138	Situ	135:138	Situ	135:138	Surface Grafting of Reverse Osmosis Membrane with Chlorhexidine Using Biopolymer Alginate Dialdehyde as a Facile Green Platform for In Situ Biofouling Control.
32701290	6	22	theme	CH-ADA-PA	907:915	arg1	membranes					917:925	the CH-ADA-PA membranes	903:925	the CH-ADA-PA membranes (38 to 42 L m-2 h-1)	903:946	Improved salt rejection performances were observed for the grafted PA membranes at the expense of negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1) compared with the pristine PA membrane (45 L m-2 h-1).
32701290	6	22	theme	CH-ADA-PA	907:915	arg1	h-1					943:945	38 to 42 L m-2 h-1	928:945	38 to 42 L m-2 h-1	928:945	Improved salt rejection performances were observed for the grafted PA membranes at the expense of negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1) compared with the pristine PA membrane (45 L m-2 h-1).
32701290	8	23	theme	grafted	1328:1334	arg1	membranes					1339:1347	the grafted PA membranes	1324:1347	the grafted PA membranes	1324:1347	Organic fouling behaviors with a protein (bovine serum albumin, BSA) and a surfactant (dodecyl trimethylammonium bromide, DTAB) were investigated as typical foulants for the grafted PA membranes.
32701290	0	24	theme	Alginate	81:88	arg1	Platform					119:126	a Facile Green Platform	104:126	a Facile Green Platform for In Situ Biofouling Control	104:157	Surface Grafting of Reverse Osmosis Membrane with Chlorhexidine Using Biopolymer Alginate Dialdehyde as a Facile Green Platform for In Situ Biofouling Control.
32701290	0	24	theme	Alginate	81:88	arg1	Dialdehyde					90:99	Biopolymer Alginate Dialdehyde	70:99	Biopolymer Alginate Dialdehyde	70:99	Surface Grafting of Reverse Osmosis Membrane with Chlorhexidine Using Biopolymer Alginate Dialdehyde as a Facile Green Platform for In Situ Biofouling Control.
32701290	1	25	theme	polyamide	340:348	arg1	membranes					376:384	composite (TFC) polyamide (PA) reverse osmosis (RO) membranes	324:384	composite (TFC) polyamide (PA) reverse osmosis (RO) membranes	324:384	We report a new robust and green facile platform for nonoxidizing chemical grafting to simultaneously improve antifouling and antibacterial properties of thin film composite (TFC) polyamide (PA) reverse osmosis (RO) membranes.
32701290	10	26	from	application	1615:1625	arg1	control					1650:1656	RO membrane fouling control	1630:1656	RO membrane fouling control	1630:1656	Hence, these results pave the way for a new robust and green bioinspired route for practical application in RO membrane fouling control.
32701290	6	27	dep	42	934:935	arg1	to					931:932	to	931:932	to	931:932	Improved salt rejection performances were observed for the grafted PA membranes at the expense of negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1) compared with the pristine PA membrane (45 L m-2 h-1).
32701290	1	28	theme	PA	351:352	arg1	membranes					376:384	composite (TFC) polyamide (PA) reverse osmosis (RO) membranes	324:384	composite (TFC) polyamide (PA) reverse osmosis (RO) membranes	324:384	We report a new robust and green facile platform for nonoxidizing chemical grafting to simultaneously improve antifouling and antibacterial properties of thin film composite (TFC) polyamide (PA) reverse osmosis (RO) membranes.
32701290	9	29	theme	CH-ADA-PA	1381:1389	arg1	membranes					1391:1399	the CH-ADA-PA membranes	1377:1399	the CH-ADA-PA membranes	1377:1399	The results indicated that the CH-ADA-PA membranes showed the best antifouling performance followed by the ADA-PA membranes, the pristine membrane being the most inferior.
32701290	6	30	theme	flux	889:892	arg1	drop					894:897	negligible flux drop	878:897	negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1)	878:946	Improved salt rejection performances were observed for the grafted PA membranes at the expense of negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1) compared with the pristine PA membrane (45 L m-2 h-1).
32701290	2	31	theme	graft	459:463	arg1	chemical					500:507	chemical	500:507	chemical	500:507	In this work, alginate dialdehyde (ADA) was used as a green platform to graft chlorhexidine (CH), a nonoxidizing chemical, on TFC-RO membrane surface.
32701290	2	31	theme	graft	459:463	arg1	CH					480:481	CH	480:481	CH	480:481	In this work, alginate dialdehyde (ADA) was used as a green platform to graft chlorhexidine (CH), a nonoxidizing chemical, on TFC-RO membrane surface.
32701290	2	31	theme	graft	459:463	arg1	chlorhexidine					465:477	graft chlorhexidine	459:477	graft chlorhexidine (CH)	459:482	In this work, alginate dialdehyde (ADA) was used as a green platform to graft chlorhexidine (CH), a nonoxidizing chemical, on TFC-RO membrane surface.
32701290	4	32	theme	contact	682:688	arg1	angle					690:694	contact angle	682:694	contact angle	682:694	The modified membrane surfaces were characterized using XPS, FT-IR, AFM, SEM-EDS, contact angle, and zeta potential analysis.
32701290	7	33	theme	antibacterial	1045:1057	arg1	activity					1059:1066	excellent antibacterial activity	1035:1066	excellent antibacterial activity against E. coli	1035:1082	All the CH-ADA-PA membranes had excellent antibacterial activity against E. coli along with a highly superior resistance to the formation of biofilms.
32701290	1	34	theme	reverse	355:361	arg1	membranes					376:384	composite (TFC) polyamide (PA) reverse osmosis (RO) membranes	324:384	composite (TFC) polyamide (PA) reverse osmosis (RO) membranes	324:384	We report a new robust and green facile platform for nonoxidizing chemical grafting to simultaneously improve antifouling and antibacterial properties of thin film composite (TFC) polyamide (PA) reverse osmosis (RO) membranes.
32701290	0	35	theme	Surface	0:6	arg1	Grafting					8:15	Surface Grafting	0:15	Surface Grafting of Reverse Osmosis Membrane with Chlorhexidine Using Biopolymer Alginate Dialdehyde as a Facile Green Platform for In Situ Biofouling Control.	0:158	Surface Grafting of Reverse Osmosis Membrane with Chlorhexidine Using Biopolymer Alginate Dialdehyde as a Facile Green Platform for In Situ Biofouling Control.
32701290	2	36	theme	alginate	401:408	arg1	dialdehyde					410:419	alginate dialdehyde	401:419	alginate dialdehyde (ADA)	401:425	In this work, alginate dialdehyde (ADA) was used as a green platform to graft chlorhexidine (CH), a nonoxidizing chemical, on TFC-RO membrane surface.
32701290	2	36	theme	alginate	401:408	arg1	platform					447:454	a green platform	439:454	a green platform to graft chlorhexidine (CH), a nonoxidizing chemical, on TFC-RO membrane surface	439:535	In this work, alginate dialdehyde (ADA) was used as a green platform to graft chlorhexidine (CH), a nonoxidizing chemical, on TFC-RO membrane surface.
32701290	2	36	theme	alginate	401:408	arg1	ADA					422:424	ADA	422:424	ADA	422:424	In this work, alginate dialdehyde (ADA) was used as a green platform to graft chlorhexidine (CH), a nonoxidizing chemical, on TFC-RO membrane surface.
32701290	6	37	from	expense	867:873	arg1	membranes					850:858	the grafted PA membranes	835:858	the grafted PA membranes at the expense of negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1)	835:946	Improved salt rejection performances were observed for the grafted PA membranes at the expense of negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1) compared with the pristine PA membrane (45 L m-2 h-1).
32701290	7	38	theme	CH-ADA-PA	1011:1019	arg1	membranes					1021:1029	All the CH-ADA-PA membranes	1003:1029	All the CH-ADA-PA membranes	1003:1029	All the CH-ADA-PA membranes had excellent antibacterial activity against E. coli along with a highly superior resistance to the formation of biofilms.
32701290	6	39	theme	L	937:937	arg1	membranes					917:925	the CH-ADA-PA membranes	903:925	the CH-ADA-PA membranes (38 to 42 L m-2 h-1)	903:946	Improved salt rejection performances were observed for the grafted PA membranes at the expense of negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1) compared with the pristine PA membrane (45 L m-2 h-1).
32701290	6	39	theme	L	937:937	arg1	h-1					943:945	38 to 42 L m-2 h-1	928:945	38 to 42 L m-2 h-1	928:945	Improved salt rejection performances were observed for the grafted PA membranes at the expense of negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1) compared with the pristine PA membrane (45 L m-2 h-1).
32701290	0	40	theme	Osmosis	28:34	arg1	Membrane					36:43	Reverse Osmosis Membrane	20:43	Reverse Osmosis Membrane	20:43	Surface Grafting of Reverse Osmosis Membrane with Chlorhexidine Using Biopolymer Alginate Dialdehyde as a Facile Green Platform for In Situ Biofouling Control.
32701290	1	41	theme	thin	314:317	arg1	film					319:322	thin film	314:322	thin film	314:322	We report a new robust and green facile platform for nonoxidizing chemical grafting to simultaneously improve antifouling and antibacterial properties of thin film composite (TFC) polyamide (PA) reverse osmosis (RO) membranes.
32701290	8	42	theme	dodecyl	1241:1247	arg1	bromide					1267:1273	dodecyl trimethylammonium bromide	1241:1273	dodecyl trimethylammonium bromide	1241:1273	Organic fouling behaviors with a protein (bovine serum albumin, BSA) and a surfactant (dodecyl trimethylammonium bromide, DTAB) were investigated as typical foulants for the grafted PA membranes.
32701290	8	42	theme	dodecyl	1241:1247	arg1	DTAB					1276:1279	DTAB	1276:1279	DTAB	1276:1279	Organic fouling behaviors with a protein (bovine serum albumin, BSA) and a surfactant (dodecyl trimethylammonium bromide, DTAB) were investigated as typical foulants for the grafted PA membranes.
32701290	1	43	theme	composite	324:332	arg1	membranes					376:384	composite (TFC) polyamide (PA) reverse osmosis (RO) membranes	324:384	composite (TFC) polyamide (PA) reverse osmosis (RO) membranes	324:384	We report a new robust and green facile platform for nonoxidizing chemical grafting to simultaneously improve antifouling and antibacterial properties of thin film composite (TFC) polyamide (PA) reverse osmosis (RO) membranes.
32701290	9	44	theme	ADA-PA	1457:1462	arg1	membranes					1464:1472	the ADA-PA membranes	1453:1472	the ADA-PA membranes	1453:1472	The results indicated that the CH-ADA-PA membranes showed the best antifouling performance followed by the ADA-PA membranes, the pristine membrane being the most inferior.
32701290	6	45	theme	L	991:991	arg1	h-1					997:999	45 L m-2 h-1	988:999	45 L m-2 h-1	988:999	Improved salt rejection performances were observed for the grafted PA membranes at the expense of negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1) compared with the pristine PA membrane (45 L m-2 h-1).
32701290	6	45	theme	L	991:991	arg1	membrane					978:985	the pristine PA membrane	962:985	the pristine PA membrane (45 L m-2 h-1)	962:1000	Improved salt rejection performances were observed for the grafted PA membranes at the expense of negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1) compared with the pristine PA membrane (45 L m-2 h-1).
32701290	8	46	with	behaviors	1170:1178	arg1	surfactant					1229:1238	a surfactant	1227:1238	a surfactant (dodecyl trimethylammonium bromide, DTAB)	1227:1280	Organic fouling behaviors with a protein (bovine serum albumin, BSA) and a surfactant (dodecyl trimethylammonium bromide, DTAB) were investigated as typical foulants for the grafted PA membranes.
32701290	8	46	with	behaviors	1170:1178	arg1	protein					1187:1193	a protein	1185:1193	a protein (bovine serum albumin, BSA)	1185:1221	Organic fouling behaviors with a protein (bovine serum albumin, BSA) and a surfactant (dodecyl trimethylammonium bromide, DTAB) were investigated as typical foulants for the grafted PA membranes.
32701290	4	47	theme	zeta	701:704	arg1	analysis					716:723	zeta potential analysis	701:723	zeta potential analysis	701:723	The modified membrane surfaces were characterized using XPS, FT-IR, AFM, SEM-EDS, contact angle, and zeta potential analysis.
32701290	10	48	theme	new	1562:1564	arg1	route					1595:1599	a new robust and green bioinspired route	1560:1599	a new robust and green bioinspired route for practical application in RO membrane fouling control	1560:1656	Hence, these results pave the way for a new robust and green bioinspired route for practical application in RO membrane fouling control.
32701290	1	49	theme	chemical	226:233	arg1	grafting					235:242	nonoxidizing chemical grafting	213:242	nonoxidizing chemical grafting	213:242	We report a new robust and green facile platform for nonoxidizing chemical grafting to simultaneously improve antifouling and antibacterial properties of thin film composite (TFC) polyamide (PA) reverse osmosis (RO) membranes.
32701290	9	50	theme	pristine	1479:1486	arg1	membrane					1488:1495	the pristine membrane	1475:1495	the pristine membrane	1475:1495	The results indicated that the CH-ADA-PA membranes showed the best antifouling performance followed by the ADA-PA membranes, the pristine membrane being the most inferior.
32701290	9	50	theme	pristine	1479:1486	arg1	inferior					1512:1519	inferior	1512:1519	inferior	1512:1519	The results indicated that the CH-ADA-PA membranes showed the best antifouling performance followed by the ADA-PA membranes, the pristine membrane being the most inferior.
32701290	6	51	theme	PA	975:976	arg1	h-1					997:999	45 L m-2 h-1	988:999	45 L m-2 h-1	988:999	Improved salt rejection performances were observed for the grafted PA membranes at the expense of negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1) compared with the pristine PA membrane (45 L m-2 h-1).
32701290	6	51	theme	PA	975:976	arg1	membrane					978:985	the pristine PA membrane	962:985	the pristine PA membrane (45 L m-2 h-1)	962:1000	Improved salt rejection performances were observed for the grafted PA membranes at the expense of negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1) compared with the pristine PA membrane (45 L m-2 h-1).
32701290	2	52	used	used	431:434	arg2	platform					447:454	a green platform	439:454	a green platform to graft chlorhexidine (CH), a nonoxidizing chemical, on TFC-RO membrane surface	439:535	In this work, alginate dialdehyde (ADA) was used as a green platform to graft chlorhexidine (CH), a nonoxidizing chemical, on TFC-RO membrane surface.
32701290	2	52	used	used	431:434	arg2	ADA					422:424	ADA	422:424	ADA	422:424	In this work, alginate dialdehyde (ADA) was used as a green platform to graft chlorhexidine (CH), a nonoxidizing chemical, on TFC-RO membrane surface.
32701290	2	52	used	used	431:434	arg2	dialdehyde					410:419	alginate dialdehyde	401:419	alginate dialdehyde (ADA)	401:425	In this work, alginate dialdehyde (ADA) was used as a green platform to graft chlorhexidine (CH), a nonoxidizing chemical, on TFC-RO membrane surface.
32701290	10	53	theme	bioinspired	1583:1593	arg1	route					1595:1599	a new robust and green bioinspired route	1560:1599	a new robust and green bioinspired route for practical application in RO membrane fouling control	1560:1656	Hence, these results pave the way for a new robust and green bioinspired route for practical application in RO membrane fouling control.
32701290	2	54	theme	TFC-RO	513:518	arg1	surface					529:535	TFC-RO membrane surface	513:535	TFC-RO membrane surface	513:535	In this work, alginate dialdehyde (ADA) was used as a green platform to graft chlorhexidine (CH), a nonoxidizing chemical, on TFC-RO membrane surface.
32701290	1	55	theme	nonoxidizing	213:224	arg1	grafting					235:242	nonoxidizing chemical grafting	213:242	nonoxidizing chemical grafting	213:242	We report a new robust and green facile platform for nonoxidizing chemical grafting to simultaneously improve antifouling and antibacterial properties of thin film composite (TFC) polyamide (PA) reverse osmosis (RO) membranes.
32701290	6	56	theme	salt	789:792	arg1	performances					804:815	Improved salt rejection performances	780:815	Improved salt rejection performances	780:815	Improved salt rejection performances were observed for the grafted PA membranes at the expense of negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1) compared with the pristine PA membrane (45 L m-2 h-1).
32701290	1	57	theme	new	172:174	arg1	platform					200:207	a new robust and green facile platform	170:207	a new robust and green facile platform for nonoxidizing chemical grafting to simultaneously improve antifouling and antibacterial properties of thin film composite (TFC) polyamide (PA) reverse osmosis (RO) membranes	170:384	We report a new robust and green facile platform for nonoxidizing chemical grafting to simultaneously improve antifouling and antibacterial properties of thin film composite (TFC) polyamide (PA) reverse osmosis (RO) membranes.
32701290	6	58	theme	negligible	878:887	arg1	drop					894:897	negligible flux drop	878:897	negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1)	878:946	Improved salt rejection performances were observed for the grafted PA membranes at the expense of negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1) compared with the pristine PA membrane (45 L m-2 h-1).
32701290	5	59	theme	simple	728:733	arg1	reaction					756:763	A simple two-step Schiff base reaction	726:763	A simple two-step Schiff base reaction	726:763	A simple two-step Schiff base reaction was performed.
32701290	1	60	theme	RO	372:373	arg1	membranes					376:384	composite (TFC) polyamide (PA) reverse osmosis (RO) membranes	324:384	composite (TFC) polyamide (PA) reverse osmosis (RO) membranes	324:384	We report a new robust and green facile platform for nonoxidizing chemical grafting to simultaneously improve antifouling and antibacterial properties of thin film composite (TFC) polyamide (PA) reverse osmosis (RO) membranes.
32701290	10	61	theme	fouling	1642:1648	arg1	control					1650:1656	RO membrane fouling control	1630:1656	RO membrane fouling control	1630:1656	Hence, these results pave the way for a new robust and green bioinspired route for practical application in RO membrane fouling control.
32701290	5	62	theme	Schiff	744:749	arg1	reaction					756:763	A simple two-step Schiff base reaction	726:763	A simple two-step Schiff base reaction	726:763	A simple two-step Schiff base reaction was performed.
32701290	2	63	from	platform	447:454	arg1	surface					529:535	TFC-RO membrane surface	513:535	TFC-RO membrane surface	513:535	In this work, alginate dialdehyde (ADA) was used as a green platform to graft chlorhexidine (CH), a nonoxidizing chemical, on TFC-RO membrane surface.
32701290	10	64	theme	RO	1630:1631	arg1	control					1650:1656	RO membrane fouling control	1630:1656	RO membrane fouling control	1630:1656	Hence, these results pave the way for a new robust and green bioinspired route for practical application in RO membrane fouling control.
32701290	6	65	theme	PA	847:848	arg1	membranes					850:858	the grafted PA membranes	835:858	the grafted PA membranes at the expense of negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1)	835:946	Improved salt rejection performances were observed for the grafted PA membranes at the expense of negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1) compared with the pristine PA membrane (45 L m-2 h-1).
32701290	8	66	theme	bovine	1196:1201	arg1	BSA					1218:1220	BSA	1218:1220	BSA	1218:1220	Organic fouling behaviors with a protein (bovine serum albumin, BSA) and a surfactant (dodecyl trimethylammonium bromide, DTAB) were investigated as typical foulants for the grafted PA membranes.
32701290	8	66	theme	bovine	1196:1201	arg1	albumin					1209:1215	bovine serum albumin	1196:1215	bovine serum albumin	1196:1215	Organic fouling behaviors with a protein (bovine serum albumin, BSA) and a surfactant (dodecyl trimethylammonium bromide, DTAB) were investigated as typical foulants for the grafted PA membranes.
32701290	8	67	theme	Organic	1154:1160	arg1	foulants					1311:1318	typical foulants	1303:1318	typical foulants for the grafted PA membranes	1303:1347	Organic fouling behaviors with a protein (bovine serum albumin, BSA) and a surfactant (dodecyl trimethylammonium bromide, DTAB) were investigated as typical foulants for the grafted PA membranes.
32701290	8	67	theme	Organic	1154:1160	arg1	behaviors					1170:1178	Organic fouling behaviors	1154:1178	Organic fouling behaviors with a protein (bovine serum albumin, BSA) and a surfactant (dodecyl trimethylammonium bromide, DTAB)	1154:1280	Organic fouling behaviors with a protein (bovine serum albumin, BSA) and a surfactant (dodecyl trimethylammonium bromide, DTAB) were investigated as typical foulants for the grafted PA membranes.
32701290	0	68	theme	Biopolymer	70:79	arg1	Platform					119:126	a Facile Green Platform	104:126	a Facile Green Platform for In Situ Biofouling Control	104:157	Surface Grafting of Reverse Osmosis Membrane with Chlorhexidine Using Biopolymer Alginate Dialdehyde as a Facile Green Platform for In Situ Biofouling Control.
32701290	0	68	theme	Biopolymer	70:79	arg1	Dialdehyde					90:99	Biopolymer Alginate Dialdehyde	70:99	Biopolymer Alginate Dialdehyde	70:99	Surface Grafting of Reverse Osmosis Membrane with Chlorhexidine Using Biopolymer Alginate Dialdehyde as a Facile Green Platform for In Situ Biofouling Control.
32701290	2	69	theme	green	441:445	arg1	dialdehyde					410:419	alginate dialdehyde	401:419	alginate dialdehyde (ADA)	401:425	In this work, alginate dialdehyde (ADA) was used as a green platform to graft chlorhexidine (CH), a nonoxidizing chemical, on TFC-RO membrane surface.
32701290	2	69	theme	green	441:445	arg1	platform					447:454	a green platform	439:454	a green platform to graft chlorhexidine (CH), a nonoxidizing chemical, on TFC-RO membrane surface	439:535	In this work, alginate dialdehyde (ADA) was used as a green platform to graft chlorhexidine (CH), a nonoxidizing chemical, on TFC-RO membrane surface.
32701290	8	70	theme	PA	1336:1337	arg1	membranes					1339:1347	the grafted PA membranes	1324:1347	the grafted PA membranes	1324:1347	Organic fouling behaviors with a protein (bovine serum albumin, BSA) and a surfactant (dodecyl trimethylammonium bromide, DTAB) were investigated as typical foulants for the grafted PA membranes.
32701290	8	71	dep	surfactant	1229:1238	arg1	bromide					1267:1273	dodecyl trimethylammonium bromide	1241:1273	dodecyl trimethylammonium bromide	1241:1273	Organic fouling behaviors with a protein (bovine serum albumin, BSA) and a surfactant (dodecyl trimethylammonium bromide, DTAB) were investigated as typical foulants for the grafted PA membranes.
32701290	8	71	dep	surfactant	1229:1238	arg1	DTAB					1276:1279	DTAB	1276:1279	DTAB	1276:1279	Organic fouling behaviors with a protein (bovine serum albumin, BSA) and a surfactant (dodecyl trimethylammonium bromide, DTAB) were investigated as typical foulants for the grafted PA membranes.
32701290	4	72	theme	membrane	613:620	arg1	surfaces					622:629	The modified membrane surfaces	600:629	The modified membrane surfaces	600:629	The modified membrane surfaces were characterized using XPS, FT-IR, AFM, SEM-EDS, contact angle, and zeta potential analysis.
32701290	6	73	theme	drop	894:897	arg1	expense					867:873	the expense	863:873	the expense of negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1)	863:946	Improved salt rejection performances were observed for the grafted PA membranes at the expense of negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1) compared with the pristine PA membrane (45 L m-2 h-1).
32701290	0	74	theme	Green	113:117	arg1	Platform					119:126	a Facile Green Platform	104:126	a Facile Green Platform for In Situ Biofouling Control	104:157	Surface Grafting of Reverse Osmosis Membrane with Chlorhexidine Using Biopolymer Alginate Dialdehyde as a Facile Green Platform for In Situ Biofouling Control.
32701290	0	74	theme	Green	113:117	arg1	Dialdehyde					90:99	Biopolymer Alginate Dialdehyde	70:99	Biopolymer Alginate Dialdehyde	70:99	Surface Grafting of Reverse Osmosis Membrane with Chlorhexidine Using Biopolymer Alginate Dialdehyde as a Facile Green Platform for In Situ Biofouling Control.
32701290	1	75	theme	green	187:191	arg1	platform					200:207	a new robust and green facile platform	170:207	a new robust and green facile platform for nonoxidizing chemical grafting to simultaneously improve antifouling and antibacterial properties of thin film composite (TFC) polyamide (PA) reverse osmosis (RO) membranes	170:384	We report a new robust and green facile platform for nonoxidizing chemical grafting to simultaneously improve antifouling and antibacterial properties of thin film composite (TFC) polyamide (PA) reverse osmosis (RO) membranes.
32701290	8	76	theme	serum	1203:1207	arg1	BSA					1218:1220	BSA	1218:1220	BSA	1218:1220	Organic fouling behaviors with a protein (bovine serum albumin, BSA) and a surfactant (dodecyl trimethylammonium bromide, DTAB) were investigated as typical foulants for the grafted PA membranes.
32701290	8	76	theme	serum	1203:1207	arg1	albumin					1209:1215	bovine serum albumin	1196:1215	bovine serum albumin	1196:1215	Organic fouling behaviors with a protein (bovine serum albumin, BSA) and a surfactant (dodecyl trimethylammonium bromide, DTAB) were investigated as typical foulants for the grafted PA membranes.
32701290	7	77	theme	excellent	1035:1043	arg1	activity					1059:1066	excellent antibacterial activity	1035:1066	excellent antibacterial activity against E. coli	1035:1082	All the CH-ADA-PA membranes had excellent antibacterial activity against E. coli along with a highly superior resistance to the formation of biofilms.
32701290	1	78	theme	osmosis	363:369	arg1	membranes					376:384	composite (TFC) polyamide (PA) reverse osmosis (RO) membranes	324:384	composite (TFC) polyamide (PA) reverse osmosis (RO) membranes	324:384	We report a new robust and green facile platform for nonoxidizing chemical grafting to simultaneously improve antifouling and antibacterial properties of thin film composite (TFC) polyamide (PA) reverse osmosis (RO) membranes.
32701290	0	79	theme	Reverse	20:26	arg1	Membrane					36:43	Reverse Osmosis Membrane	20:43	Reverse Osmosis Membrane	20:43	Surface Grafting of Reverse Osmosis Membrane with Chlorhexidine Using Biopolymer Alginate Dialdehyde as a Facile Green Platform for In Situ Biofouling Control.
32701290	1	80	theme	antibacterial	286:298	arg1	properties					300:309	antibacterial properties	286:309	antibacterial properties of thin film	286:322	We report a new robust and green facile platform for nonoxidizing chemical grafting to simultaneously improve antifouling and antibacterial properties of thin film composite (TFC) polyamide (PA) reverse osmosis (RO) membranes.
32701290	9	81	theme	antifouling	1417:1427	arg1	performance					1429:1439	the best antifouling performance	1408:1439	the best antifouling performance followed by the ADA-PA membranes	1408:1472	The results indicated that the CH-ADA-PA membranes showed the best antifouling performance followed by the ADA-PA membranes, the pristine membrane being the most inferior.
32701290	6	82	theme	m-2	939:941	arg1	membranes					917:925	the CH-ADA-PA membranes	903:925	the CH-ADA-PA membranes (38 to 42 L m-2 h-1)	903:946	Improved salt rejection performances were observed for the grafted PA membranes at the expense of negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1) compared with the pristine PA membrane (45 L m-2 h-1).
32701290	6	82	theme	m-2	939:941	arg1	h-1					943:945	38 to 42 L m-2 h-1	928:945	38 to 42 L m-2 h-1	928:945	Improved salt rejection performances were observed for the grafted PA membranes at the expense of negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1) compared with the pristine PA membrane (45 L m-2 h-1).
32701290	0	83	theme	Membrane	36:43	arg1	Grafting					8:15	Surface Grafting	0:15	Surface Grafting of Reverse Osmosis Membrane with Chlorhexidine Using Biopolymer Alginate Dialdehyde as a Facile Green Platform for In Situ Biofouling Control.	0:158	Surface Grafting of Reverse Osmosis Membrane with Chlorhexidine Using Biopolymer Alginate Dialdehyde as a Facile Green Platform for In Situ Biofouling Control.
32701290	1	84	theme	facile	193:198	arg1	platform					200:207	a new robust and green facile platform	170:207	a new robust and green facile platform for nonoxidizing chemical grafting to simultaneously improve antifouling and antibacterial properties of thin film composite (TFC) polyamide (PA) reverse osmosis (RO) membranes	170:384	We report a new robust and green facile platform for nonoxidizing chemical grafting to simultaneously improve antifouling and antibacterial properties of thin film composite (TFC) polyamide (PA) reverse osmosis (RO) membranes.
32701290	8	85	dep	protein	1187:1193	arg1	BSA					1218:1220	BSA	1218:1220	BSA	1218:1220	Organic fouling behaviors with a protein (bovine serum albumin, BSA) and a surfactant (dodecyl trimethylammonium bromide, DTAB) were investigated as typical foulants for the grafted PA membranes.
32701290	8	85	dep	protein	1187:1193	arg1	albumin					1209:1215	bovine serum albumin	1196:1215	bovine serum albumin	1196:1215	Organic fouling behaviors with a protein (bovine serum albumin, BSA) and a surfactant (dodecyl trimethylammonium bromide, DTAB) were investigated as typical foulants for the grafted PA membranes.
32701290	7	86	theme	biofilms	1144:1151	arg1	formation					1131:1139	the formation	1127:1139	the formation of biofilms	1127:1151	All the CH-ADA-PA membranes had excellent antibacterial activity against E. coli along with a highly superior resistance to the formation of biofilms.
32701290	4	87	theme	potential	706:714	arg1	analysis					716:723	zeta potential analysis	701:723	zeta potential analysis	701:723	The modified membrane surfaces were characterized using XPS, FT-IR, AFM, SEM-EDS, contact angle, and zeta potential analysis.
32701290	0	88	theme	Facile	106:111	arg1	Platform					119:126	a Facile Green Platform	104:126	a Facile Green Platform for In Situ Biofouling Control	104:157	Surface Grafting of Reverse Osmosis Membrane with Chlorhexidine Using Biopolymer Alginate Dialdehyde as a Facile Green Platform for In Situ Biofouling Control.
32701290	0	88	theme	Facile	106:111	arg1	Dialdehyde					90:99	Biopolymer Alginate Dialdehyde	70:99	Biopolymer Alginate Dialdehyde	70:99	Surface Grafting of Reverse Osmosis Membrane with Chlorhexidine Using Biopolymer Alginate Dialdehyde as a Facile Green Platform for In Situ Biofouling Control.
32701290	1	89	theme	film	319:322	arg1	properties					300:309	antibacterial properties	286:309	antibacterial properties of thin film	286:322	We report a new robust and green facile platform for nonoxidizing chemical grafting to simultaneously improve antifouling and antibacterial properties of thin film composite (TFC) polyamide (PA) reverse osmosis (RO) membranes.
32701290	1	89	theme	film	319:322	arg1	antifouling					270:280	antifouling	270:280	antifouling	270:280	We report a new robust and green facile platform for nonoxidizing chemical grafting to simultaneously improve antifouling and antibacterial properties of thin film composite (TFC) polyamide (PA) reverse osmosis (RO) membranes.
32701290	6	90	theme	m-2	993:995	arg1	h-1					997:999	45 L m-2 h-1	988:999	45 L m-2 h-1	988:999	Improved salt rejection performances were observed for the grafted PA membranes at the expense of negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1) compared with the pristine PA membrane (45 L m-2 h-1).
32701290	6	90	theme	m-2	993:995	arg1	membrane					978:985	the pristine PA membrane	962:985	the pristine PA membrane (45 L m-2 h-1)	962:1000	Improved salt rejection performances were observed for the grafted PA membranes at the expense of negligible flux drop for the CH-ADA-PA membranes (38 to 42 L m-2 h-1) compared with the pristine PA membrane (45 L m-2 h-1).
32701290	0	91	with	Grafting	8:15	arg1	Chlorhexidine					50:62	Chlorhexidine	50:62	Chlorhexidine Using Biopolymer Alginate Dialdehyde as a Facile Green Platform for In Situ Biofouling Control	50:157	Surface Grafting of Reverse Osmosis Membrane with Chlorhexidine Using Biopolymer Alginate Dialdehyde as a Facile Green Platform for In Situ Biofouling Control.
32701290	8	92	theme	trimethylammonium	1249:1265	arg1	bromide					1267:1273	dodecyl trimethylammonium bromide	1241:1273	dodecyl trimethylammonium bromide	1241:1273	Organic fouling behaviors with a protein (bovine serum albumin, BSA) and a surfactant (dodecyl trimethylammonium bromide, DTAB) were investigated as typical foulants for the grafted PA membranes.
32701290	8	92	theme	trimethylammonium	1249:1265	arg1	DTAB					1276:1279	DTAB	1276:1279	DTAB	1276:1279	Organic fouling behaviors with a protein (bovine serum albumin, BSA) and a surfactant (dodecyl trimethylammonium bromide, DTAB) were investigated as typical foulants for the grafted PA membranes.
32701290	3	93	dep	ADA	566:568	arg1	grafting					577:584	grafting	577:584	grafting	577:584	A synergistic effect due to ADA and CH grafting was revealed.
32701290	7	94	theme	superior	1104:1111	arg1	resistance					1113:1122	a highly superior resistance	1095:1122	a highly superior resistance to the formation of biofilms	1095:1151	All the CH-ADA-PA membranes had excellent antibacterial activity against E. coli along with a highly superior resistance to the formation of biofilms.
33562827	5	0	theme	negative	713:720	arg1	influence					722:730	a negative influence	711:730	a negative influence	711:730	The extraction temperature only had a negative influence on carotenoid content.
33562827	8	1	theme	particle	1129:1136	arg1	diameter					1143:1150	particle mean diameter	1129:1150	particle mean diameter	1129:1150	The encapsulation efficiency and particle mean diameter were 89.5% and 0.78 mm, respectively.
33562827	3	2	dep	conditions	362:371	arg1	conditions					362:371	the optimal conditions	350:371	the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g)	350:471	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	3	2	dep	conditions	362:371	arg1	time					385:388	extraction time	374:388	extraction time of 35 min	374:398	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	3	2	dep	conditions	362:371	arg1	ratio					455:459	a liquid-to-solid ratio	437:459	a liquid-to-solid ratio of 15 mL/g	437:470	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	3	2	dep	conditions	362:371	arg1	temperature					412:422	extraction temperature	401:422	extraction temperature of 42 ℃	401:430	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	3	3	theme	mL/g	467:470	arg1	conditions					362:371	the optimal conditions	350:371	the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g)	350:471	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	3	3	theme	mL/g	467:470	arg1	time					385:388	extraction time	374:388	extraction time of 35 min	374:398	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	3	3	theme	mL/g	467:470	arg1	temperature					412:422	extraction temperature	401:422	extraction temperature of 42 ℃	401:430	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	3	3	theme	mL/g	467:470	arg1	ratio					455:459	a liquid-to-solid ratio	437:459	a liquid-to-solid ratio of 15 mL/g	437:470	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	6	4	theme	extraction	865:874	arg1	time					876:879	extraction time	865:879	extraction time	865:879	The impact of extraction parameters on the carotenoid content was decreased according to the following order: extraction time, liquid-to-solid ratio, and extraction temperature.
33562827	6	5	theme	extraction	769:778	arg1	parameters					780:789	extraction parameters	769:789	extraction parameters	769:789	The impact of extraction parameters on the carotenoid content was decreased according to the following order: extraction time, liquid-to-solid ratio, and extraction temperature.
33562827	9	6	theme	extract	1210:1216	arg1	presence					1194:1201	The presence	1190:1201	The presence of oil extract in Ca-alginate beads	1190:1237	The presence of oil extract in Ca-alginate beads was confirmed by Fourier-transform infrared spectroscopy.
33562827	5	7	contain	had	707:709	arg1	temperature					690:700	The extraction temperature	675:700	The extraction temperature	675:700	The extraction temperature only had a negative influence on carotenoid content.
33562827	5	7	contain	had	707:709	arg2	influence					722:730	a negative influence	711:730	a negative influence	711:730	The extraction temperature only had a negative influence on carotenoid content.
33562827	7	8	theme	Ca-alginate	933:943	arg1	beads					945:949	Ca-alginate beads	933:949	Ca-alginate beads	933:949	Ca-alginate beads were prepared using the extrusion process to increase the stability and protect the antioxidant activity of olive oil enriched with carotenoids.
33562827	3	9	theme	predicted	495:503	arg1	values					505:510	the experimental and predicted values	474:510	values	505:510	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	3	9	theme	predicted	495:503	arg1	weight					566:571	1.85 and 1.83 mg/100 g dry weight	539:571	1.85 and 1.83 mg/100 g dry weight	539:571	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	2	10	used	used	284:287	arg2	design					273:278	A central composite design	253:278	A central composite design	253:278	A central composite design was used to define the optimal conditions for their extraction.
33562827	3	11	theme	content	526:532	arg1	values					505:510	the experimental and predicted values	474:510	values	505:510	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	3	11	theme	content	526:532	arg1	weight					566:571	1.85 and 1.83 mg/100 g dry weight	539:571	1.85 and 1.83 mg/100 g dry weight	539:571	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	10	12	from	encapsulation	1376:1388	arg1	beads					1406:1410	the alginate beads	1393:1410	the alginate beads	1393:1410	The antioxidant activity of the oil enriched with carotenoids before and after encapsulation in the alginate beads was determined according to the DPPH assay.
33562827	9	13	from	presence	1194:1201	arg1	beads					1233:1237	Ca-alginate beads	1221:1237	Ca-alginate beads	1221:1237	The presence of oil extract in Ca-alginate beads was confirmed by Fourier-transform infrared spectroscopy.
33562827	0	14	from	Peel	58:61	arg1	Extraction					20:29	Ultrasound-Assisted Extraction	0:29	Ultrasound-Assisted Extraction of Carotenoids from Orange Peel Using Olive Oil and Its Encapsulation in Ca-Alginate Beads.	0:121	Ultrasound-Assisted Extraction of Carotenoids from Orange Peel Using Olive Oil and Its Encapsulation in Ca-Alginate Beads.
33562827	7	15	theme	oil	1065:1067	arg1	activity					1047:1054	the antioxidant activity	1031:1054	the antioxidant activity of olive oil enriched with carotenoids	1031:1093	Ca-alginate beads were prepared using the extrusion process to increase the stability and protect the antioxidant activity of olive oil enriched with carotenoids.
33562827	3	16	theme	experimental	478:489	arg1	values					505:510	the experimental and predicted values	474:510	values	505:510	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	3	16	theme	experimental	478:489	arg1	weight					566:571	1.85 and 1.83 mg/100 g dry weight	539:571	1.85 and 1.83 mg/100 g dry weight	539:571	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	1	17	theme	ultrasound-assisted	160:178	arg1	extraction					180:189	an ultrasound-assisted extraction	157:189	an ultrasound-assisted extraction of carotenoids from orange peel using olive oil as a solvent	157:250	The paper was aimed at developing an ultrasound-assisted extraction of carotenoids from orange peel using olive oil as a solvent.
33562827	0	18	theme	Ca-Alginate	104:114	arg1	Beads					116:120	Ca-Alginate Beads	104:120	Ca-Alginate Beads	104:120	Ultrasound-Assisted Extraction of Carotenoids from Orange Peel Using Olive Oil and Its Encapsulation in Ca-Alginate Beads.
33562827	3	19	theme	min	396:398	arg1	conditions					362:371	the optimal conditions	350:371	the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g)	350:471	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	3	19	theme	min	396:398	arg1	time					385:388	extraction time	374:388	extraction time of 35 min	374:398	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	3	19	theme	min	396:398	arg1	temperature					412:422	extraction temperature	401:422	extraction temperature of 42 ℃	401:430	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	3	19	theme	min	396:398	arg1	ratio					455:459	a liquid-to-solid ratio	437:459	a liquid-to-solid ratio of 15 mL/g	437:470	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	2	20	theme	composite	263:271	arg1	design					273:278	A central composite design	253:278	A central composite design	253:278	A central composite design was used to define the optimal conditions for their extraction.
33562827	9	21	theme	oil	1206:1208	arg1	extract					1210:1216	oil extract	1206:1216	oil extract	1206:1216	The presence of oil extract in Ca-alginate beads was confirmed by Fourier-transform infrared spectroscopy.
33562827	3	22	theme	extraction	374:383	arg1	conditions					362:371	the optimal conditions	350:371	the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g)	350:471	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	3	22	theme	extraction	374:383	arg1	time					385:388	extraction time	374:388	extraction time of 35 min	374:398	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	2	23	theme	optimal	303:309	arg1	conditions					311:320	the optimal conditions	299:320	the optimal conditions for their extraction	299:341	A central composite design was used to define the optimal conditions for their extraction.
33562827	6	24	theme	following	848:856	arg1	order					858:862	the following order	844:862	the following order	844:862	The impact of extraction parameters on the carotenoid content was decreased according to the following order: extraction time, liquid-to-solid ratio, and extraction temperature.
33562827	3	25	theme	extraction	401:410	arg1	conditions					362:371	the optimal conditions	350:371	the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g)	350:471	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	3	25	theme	extraction	401:410	arg1	temperature					412:422	extraction temperature	401:422	extraction temperature of 42 ℃	401:430	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	0	26	theme	Ultrasound-Assisted	0:18	arg1	Extraction					20:29	Ultrasound-Assisted Extraction	0:29	Ultrasound-Assisted Extraction of Carotenoids from Orange Peel Using Olive Oil and Its Encapsulation in Ca-Alginate Beads.	0:121	Ultrasound-Assisted Extraction of Carotenoids from Orange Peel Using Olive Oil and Its Encapsulation in Ca-Alginate Beads.
33562827	6	27	theme	parameters	780:789	arg1	impact					759:764	The impact	755:764	The impact of extraction parameters on the carotenoid content	755:815	The impact of extraction parameters on the carotenoid content was decreased according to the following order: extraction time, liquid-to-solid ratio, and extraction temperature.
33562827	10	28	theme	alginate	1397:1404	arg1	beads					1406:1410	the alginate beads	1393:1410	the alginate beads	1393:1410	The antioxidant activity of the oil enriched with carotenoids before and after encapsulation in the alginate beads was determined according to the DPPH assay.
33562827	10	29	theme	antioxidant	1301:1311	arg1	activity					1313:1320	The antioxidant activity	1297:1320	The antioxidant activity of the oil enriched with carotenoids before and after encapsulation in the alginate beads	1297:1410	The antioxidant activity of the oil enriched with carotenoids before and after encapsulation in the alginate beads was determined according to the DPPH assay.
33562827	1	30	theme	carotenoids	194:204	arg1	extraction					180:189	an ultrasound-assisted extraction	157:189	an ultrasound-assisted extraction of carotenoids from orange peel using olive oil as a solvent	157:250	The paper was aimed at developing an ultrasound-assisted extraction of carotenoids from orange peel using olive oil as a solvent.
33562827	0	31	theme	Carotenoids	34:44	arg1	Extraction					20:29	Ultrasound-Assisted Extraction	0:29	Ultrasound-Assisted Extraction of Carotenoids from Orange Peel Using Olive Oil and Its Encapsulation in Ca-Alginate Beads.	0:121	Ultrasound-Assisted Extraction of Carotenoids from Orange Peel Using Olive Oil and Its Encapsulation in Ca-Alginate Beads.
33562827	7	32	theme	antioxidant	1035:1045	arg1	activity					1047:1054	the antioxidant activity	1031:1054	the antioxidant activity of olive oil enriched with carotenoids	1031:1093	Ca-alginate beads were prepared using the extrusion process to increase the stability and protect the antioxidant activity of olive oil enriched with carotenoids.
33562827	5	33	theme	extraction	679:688	arg1	temperature					690:700	The extraction temperature	675:700	The extraction temperature	675:700	The extraction temperature only had a negative influence on carotenoid content.
33562827	10	34	theme	DPPH	1444:1447	arg1	assay					1449:1453	the DPPH assay	1440:1453	the DPPH assay	1440:1453	The antioxidant activity of the oil enriched with carotenoids before and after encapsulation in the alginate beads was determined according to the DPPH assay.
33562827	3	35	theme	carotenoid	515:524	arg1	content					526:532	carotenoid content	515:532	carotenoid content	515:532	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	1	36	theme	orange	211:216	arg1	peel					218:221	orange peel	211:221	orange peel using olive oil as a solvent	211:250	The paper was aimed at developing an ultrasound-assisted extraction of carotenoids from orange peel using olive oil as a solvent.
33562827	0	37	theme	Orange	51:56	arg1	Peel					58:61	Orange Peel	51:61	Orange Peel Using Olive Oil and Its Encapsulation in Ca-Alginate Beads	51:120	Ultrasound-Assisted Extraction of Carotenoids from Orange Peel Using Olive Oil and Its Encapsulation in Ca-Alginate Beads.
33562827	6	38	theme	extraction	909:918	arg1	temperature					920:930	extraction temperature	909:930	extraction temperature	909:930	The impact of extraction parameters on the carotenoid content was decreased according to the following order: extraction time, liquid-to-solid ratio, and extraction temperature.
33562827	4	39	theme	values	611:616	arg1	agreement					592:600	The agreement	588:600	The agreement of these values	588:616	The agreement of these values indicated the adequacy of the proposed regression model.
33562827	3	40	theme	℃	430:430	arg1	conditions					362:371	the optimal conditions	350:371	the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g)	350:471	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	3	40	theme	℃	430:430	arg1	time					385:388	extraction time	374:388	extraction time of 35 min	374:398	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	3	40	theme	℃	430:430	arg1	temperature					412:422	extraction temperature	401:422	extraction temperature of 42 ℃	401:430	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	3	40	theme	℃	430:430	arg1	ratio					455:459	a liquid-to-solid ratio	437:459	a liquid-to-solid ratio of 15 mL/g	437:470	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	5	41	theme	carotenoid	735:744	arg1	content					746:752	carotenoid content	735:752	carotenoid content	735:752	The extraction temperature only had a negative influence on carotenoid content.
33562827	0	42	from	Oil	75:77	arg1	Beads					116:120	Ca-Alginate Beads	104:120	Ca-Alginate Beads	104:120	Ultrasound-Assisted Extraction of Carotenoids from Orange Peel Using Olive Oil and Its Encapsulation in Ca-Alginate Beads.
33562827	3	43	theme	mg/100	553:558	arg1	values					505:510	the experimental and predicted values	474:510	values	505:510	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	3	43	theme	mg/100	553:558	arg1	weight					566:571	1.85 and 1.83 mg/100 g dry weight	539:571	1.85 and 1.83 mg/100 g dry weight	539:571	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	1	44	from	peel	218:221	arg1	extraction					180:189	an ultrasound-assisted extraction	157:189	an ultrasound-assisted extraction of carotenoids from orange peel using olive oil as a solvent	157:250	The paper was aimed at developing an ultrasound-assisted extraction of carotenoids from orange peel using olive oil as a solvent.
33562827	8	45	theme	encapsulation	1100:1112	arg1	%					1161:1161	89.5%	1157:1161	89.5%	1157:1161	The encapsulation efficiency and particle mean diameter were 89.5% and 0.78 mm, respectively.
33562827	8	45	theme	encapsulation	1100:1112	arg1	efficiency					1114:1123	The encapsulation efficiency	1096:1123	The encapsulation efficiency	1096:1123	The encapsulation efficiency and particle mean diameter were 89.5% and 0.78 mm, respectively.
33562827	4	46	theme	regression	657:666	arg1	model					668:672	the proposed regression model	644:672	the proposed regression model	644:672	The agreement of these values indicated the adequacy of the proposed regression model.
33562827	9	47	theme	infrared	1274:1281	arg1	spectroscopy					1283:1294	Fourier-transform infrared spectroscopy	1256:1294	Fourier-transform infrared spectroscopy	1256:1294	The presence of oil extract in Ca-alginate beads was confirmed by Fourier-transform infrared spectroscopy.
33562827	6	48	theme	carotenoid	798:807	arg1	content					809:815	the carotenoid content	794:815	the carotenoid content	794:815	The impact of extraction parameters on the carotenoid content was decreased according to the following order: extraction time, liquid-to-solid ratio, and extraction temperature.
33562827	6	49	from	impact	759:764	arg1	content					809:815	the carotenoid content	794:815	the carotenoid content	794:815	The impact of extraction parameters on the carotenoid content was decreased according to the following order: extraction time, liquid-to-solid ratio, and extraction temperature.
33562827	4	50	theme	proposed	648:655	arg1	model					668:672	the proposed regression model	644:672	the proposed regression model	644:672	The agreement of these values indicated the adequacy of the proposed regression model.
33562827	6	51	theme	liquid-to-solid	882:896	arg1	ratio					898:902	liquid-to-solid ratio	882:902	liquid-to-solid ratio	882:902	The impact of extraction parameters on the carotenoid content was decreased according to the following order: extraction time, liquid-to-solid ratio, and extraction temperature.
33562827	8	52	theme	mean	1138:1141	arg1	diameter					1143:1150	particle mean diameter	1129:1150	particle mean diameter	1129:1150	The encapsulation efficiency and particle mean diameter were 89.5% and 0.78 mm, respectively.
33562827	2	53	theme	central	255:261	arg1	design					273:278	A central composite design	253:278	A central composite design	253:278	A central composite design was used to define the optimal conditions for their extraction.
33562827	7	54	theme	extrusion	975:983	arg1	process					985:991	the extrusion process	971:991	the extrusion process	971:991	Ca-alginate beads were prepared using the extrusion process to increase the stability and protect the antioxidant activity of olive oil enriched with carotenoids.
33562827	3	55	theme	liquid-to-solid	439:453	arg1	conditions					362:371	the optimal conditions	350:371	the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g)	350:471	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	3	55	theme	liquid-to-solid	439:453	arg1	ratio					455:459	a liquid-to-solid ratio	437:459	a liquid-to-solid ratio of 15 mL/g	437:470	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	9	56	attach	presence	1194:1201	arg1	beads					1233:1237	Ca-alginate beads	1221:1237	Ca-alginate beads	1221:1237	The presence of oil extract in Ca-alginate beads was confirmed by Fourier-transform infrared spectroscopy.
33562827	9	56	attach	presence	1194:1201	arg2	extract					1210:1216	oil extract	1206:1216	oil extract	1206:1216	The presence of oil extract in Ca-alginate beads was confirmed by Fourier-transform infrared spectroscopy.
33562827	1	57	theme	olive	229:233	arg1	oil					235:237	olive oil	229:237	olive oil	229:237	The paper was aimed at developing an ultrasound-assisted extraction of carotenoids from orange peel using olive oil as a solvent.
33562827	1	57	theme	olive	229:233	arg1	solvent					244:250	a solvent	242:250	a solvent	242:250	The paper was aimed at developing an ultrasound-assisted extraction of carotenoids from orange peel using olive oil as a solvent.
33562827	10	58	theme	oil	1329:1331	arg1	activity					1313:1320	The antioxidant activity	1297:1320	The antioxidant activity of the oil enriched with carotenoids before and after encapsulation in the alginate beads	1297:1410	The antioxidant activity of the oil enriched with carotenoids before and after encapsulation in the alginate beads was determined according to the DPPH assay.
33562827	4	59	theme	model	668:672	arg1	adequacy					632:639	the adequacy	628:639	the adequacy of the proposed regression model	628:672	The agreement of these values indicated the adequacy of the proposed regression model.
33562827	7	60	theme	olive	1059:1063	arg1	oil					1065:1067	olive oil	1059:1067	olive oil enriched with carotenoids	1059:1093	Ca-alginate beads were prepared using the extrusion process to increase the stability and protect the antioxidant activity of olive oil enriched with carotenoids.
33562827	0	61	from	Encapsulation	87:99	arg1	Beads					116:120	Ca-Alginate Beads	104:120	Ca-Alginate Beads	104:120	Ultrasound-Assisted Extraction of Carotenoids from Orange Peel Using Olive Oil and Its Encapsulation in Ca-Alginate Beads.
33562827	3	62	theme	g	560:560	arg1	values					505:510	the experimental and predicted values	474:510	values	505:510	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	3	62	theme	g	560:560	arg1	weight					566:571	1.85 and 1.83 mg/100 g dry weight	539:571	1.85 and 1.83 mg/100 g dry weight	539:571	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	9	63	theme	Ca-alginate	1221:1231	arg1	beads					1233:1237	Ca-alginate beads	1221:1237	Ca-alginate beads	1221:1237	The presence of oil extract in Ca-alginate beads was confirmed by Fourier-transform infrared spectroscopy.
33562827	6	64	dep	order	858:862	arg1	temperature					920:930	extraction temperature	909:930	extraction temperature	909:930	The impact of extraction parameters on the carotenoid content was decreased according to the following order: extraction time, liquid-to-solid ratio, and extraction temperature.
33562827	6	64	dep	order	858:862	arg1	time					876:879	extraction time	865:879	extraction time	865:879	The impact of extraction parameters on the carotenoid content was decreased according to the following order: extraction time, liquid-to-solid ratio, and extraction temperature.
33562827	6	64	dep	order	858:862	arg1	ratio					898:902	liquid-to-solid ratio	882:902	liquid-to-solid ratio	882:902	The impact of extraction parameters on the carotenoid content was decreased according to the following order: extraction time, liquid-to-solid ratio, and extraction temperature.
33562827	0	65	theme	Olive	69:73	arg1	Oil					75:77	Olive Oil	69:77	Olive Oil	69:77	Ultrasound-Assisted Extraction of Carotenoids from Orange Peel Using Olive Oil and Its Encapsulation in Ca-Alginate Beads.
33562827	9	66	theme	Fourier-transform	1256:1272	arg1	spectroscopy					1283:1294	Fourier-transform infrared spectroscopy	1256:1294	Fourier-transform infrared spectroscopy	1256:1294	The presence of oil extract in Ca-alginate beads was confirmed by Fourier-transform infrared spectroscopy.
33562827	3	67	theme	dry	562:564	arg1	values					505:510	the experimental and predicted values	474:510	values	505:510	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	3	67	theme	dry	562:564	arg1	weight					566:571	1.85 and 1.83 mg/100 g dry weight	539:571	1.85 and 1.83 mg/100 g dry weight	539:571	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	3	68	theme	optimal	354:360	arg1	conditions					362:371	the optimal conditions	350:371	the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g)	350:471	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	3	68	theme	optimal	354:360	arg1	time					385:388	extraction time	374:388	extraction time of 35 min	374:398	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	3	68	theme	optimal	354:360	arg1	ratio					455:459	a liquid-to-solid ratio	437:459	a liquid-to-solid ratio of 15 mL/g	437:470	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
33562827	3	68	theme	optimal	354:360	arg1	temperature					412:422	extraction temperature	401:422	extraction temperature of 42 ℃	401:430	Under the optimal conditions (extraction time of 35 min, extraction temperature of 42 ℃, and a liquid-to-solid ratio of 15 mL/g), the experimental and predicted values of carotenoid content were 1.85 and 1.83 mg/100 g dry weight, respectively.
34355721	5	0	theme	genes	730:734	arg1	levels					720:725	the expression levels	705:725	the expression levels of genes related to histological damage	705:765	After treatment with BR14, the expression levels of genes related to histological damage were all upregulated.
34355721	6	1	theme	IL-10	884:888	arg1	expression					870:879	the expression	866:879	the expression of IL-10	866:888	BR14 significantly attenuated the expression levels of TNF-α and IL-6, while the expression of IL-10 was upregulated.
34355721	7	2	theme	Escherichia-Shigella	1115:1134	arg1	abundance					1088:1096	the abundance	1084:1096	the abundance of Turicibacter, Escherichia-Shigella, Desulfovibrio, and Lachnospiraceae	1084:1170	Additionally, BR14 rebalanced the intestinal microbial composition of colitis mice by increasing the abundance of Muribaculaceae, Lactobacillus and Rikenellaceae and decreasing the abundance of Turicibacter, Escherichia-Shigella, Desulfovibrio, and Lachnospiraceae.
34355721	3	3	theme	anti-inflammatory	492:508	arg1	activity					510:517	anti-inflammatory activity	492:517	anti-inflammatory activity	492:517	Among 16 yeast strains, BR14, BR54, and BR174 strains showed good stress-resistant capacity, anti-inflammatory activity, and little toxicity to macrophages.
34355721	1	4	theme	yeast	245:249	arg1	species					251:257	other yeast species	239:257	other yeast species	239:257	The probiotic Saccharomyces boulardii has been widely used in colitis treatment; however, the beneficial effects of other yeast species are rarely studied.
34355721	5	5	theme	related	736:742	arg1	genes					730:734	genes	730:734	genes related to histological damage	730:765	After treatment with BR14, the expression levels of genes related to histological damage were all upregulated.
34355721	4	6	theme	colon	660:664	arg1	shortening					666:675	colon shortening	660:675	colon shortening	660:675	As for the colitis mice, BR14 inhibited weight loss the most, as well as the disease activity index and colon shortening.
34355721	1	7	theme	species	251:257	arg1	effects					228:234	the beneficial effects	213:234	the beneficial effects of other yeast species	213:257	The probiotic Saccharomyces boulardii has been widely used in colitis treatment; however, the beneficial effects of other yeast species are rarely studied.
34355721	7	8	theme	intestinal	941:950	arg1	composition					962:972	the intestinal microbial composition	937:972	the intestinal microbial composition of colitis mice	937:988	Additionally, BR14 rebalanced the intestinal microbial composition of colitis mice by increasing the abundance of Muribaculaceae, Lactobacillus and Rikenellaceae and decreasing the abundance of Turicibacter, Escherichia-Shigella, Desulfovibrio, and Lachnospiraceae.
34355721	3	9	theme	stress-resistant	465:480	arg1	capacity					482:489	good stress-resistant capacity	460:489	good stress-resistant capacity	460:489	Among 16 yeast strains, BR14, BR54, and BR174 strains showed good stress-resistant capacity, anti-inflammatory activity, and little toxicity to macrophages.
34355721	3	10	theme	little	524:529	arg1	toxicity					531:538	little toxicity	524:538	little toxicity	524:538	Among 16 yeast strains, BR14, BR54, and BR174 strains showed good stress-resistant capacity, anti-inflammatory activity, and little toxicity to macrophages.
34355721	4	11	theme	activity	641:648	arg1	index					650:654	the disease activity index	629:654	the disease activity index	629:654	As for the colitis mice, BR14 inhibited weight loss the most, as well as the disease activity index and colon shortening.
34355721	5	12	theme	histological	747:758	arg1	damage					760:765	histological damage	747:765	histological damage	747:765	After treatment with BR14, the expression levels of genes related to histological damage were all upregulated.
34355721	0	13	theme	intestinal	100:109	arg1	microbiota					111:120	the intestinal microbiota	96:120	the intestinal microbiota	96:120	Probiotic yeast BR14 ameliorates DSS-induced colitis by restoring the gut barrier and adjusting the intestinal microbiota.
34355721	0	14	theme	yeast	10:14	arg1	BR14					16:19	Probiotic yeast BR14	0:19	Probiotic yeast BR14	0:19	Probiotic yeast BR14 ameliorates DSS-induced colitis by restoring the gut barrier and adjusting the intestinal microbiota.
34355721	6	15	theme	IL-6	854:857	arg1	levels					834:839	the expression levels	819:839	the expression levels of TNF-α and IL-6	819:857	BR14 significantly attenuated the expression levels of TNF-α and IL-6, while the expression of IL-10 was upregulated.
34355721	1	16	theme	colitis	185:191	arg1	treatment					193:201	colitis treatment	185:201	colitis treatment	185:201	The probiotic Saccharomyces boulardii has been widely used in colitis treatment; however, the beneficial effects of other yeast species are rarely studied.
34355721	0	17	theme	Probiotic	0:8	arg1	BR14					16:19	Probiotic yeast BR14	0:19	Probiotic yeast BR14	0:19	Probiotic yeast BR14 ameliorates DSS-induced colitis by restoring the gut barrier and adjusting the intestinal microbiota.
34355721	7	18	theme	Lachnospiraceae	1156:1170	arg1	abundance					1088:1096	the abundance	1084:1096	the abundance of Turicibacter, Escherichia-Shigella, Desulfovibrio, and Lachnospiraceae	1084:1170	Additionally, BR14 rebalanced the intestinal microbial composition of colitis mice by increasing the abundance of Muribaculaceae, Lactobacillus and Rikenellaceae and decreasing the abundance of Turicibacter, Escherichia-Shigella, Desulfovibrio, and Lachnospiraceae.
34355721	2	19	theme	stress	315:320	arg1	tolerance					322:330	great stress tolerance	309:330	great stress tolerance	309:330	Saccharomyces cerevisiae with great stress tolerance and potential in colitis treatment was investigated in this study.
34355721	4	20	theme	disease	633:639	arg1	index					650:654	the disease activity index	629:654	the disease activity index	629:654	As for the colitis mice, BR14 inhibited weight loss the most, as well as the disease activity index and colon shortening.
34355721	7	21	theme	Rikenellaceae	1055:1067	arg1	abundance					1008:1016	the abundance	1004:1016	the abundance of Muribaculaceae, Lactobacillus and Rikenellaceae	1004:1067	Additionally, BR14 rebalanced the intestinal microbial composition of colitis mice by increasing the abundance of Muribaculaceae, Lactobacillus and Rikenellaceae and decreasing the abundance of Turicibacter, Escherichia-Shigella, Desulfovibrio, and Lachnospiraceae.
34355721	2	22	theme	great	309:313	arg1	tolerance					322:330	great stress tolerance	309:330	great stress tolerance	309:330	Saccharomyces cerevisiae with great stress tolerance and potential in colitis treatment was investigated in this study.
34355721	5	23	with	treatment	684:692	arg1	BR14					699:702	BR14	699:702	BR14	699:702	After treatment with BR14, the expression levels of genes related to histological damage were all upregulated.
34355721	8	24	theme	great	1200:1204	arg1	potential					1206:1214	great potential	1200:1214	great potential	1200:1214	In summary, BR14 exhibited great potential in alleviating colitis through restoring the gut barrier and adjusting the intestinal microbiota.
34355721	7	25	theme	Lactobacillus	1037:1049	arg1	abundance					1008:1016	the abundance	1004:1016	the abundance of Muribaculaceae, Lactobacillus and Rikenellaceae	1004:1067	Additionally, BR14 rebalanced the intestinal microbial composition of colitis mice by increasing the abundance of Muribaculaceae, Lactobacillus and Rikenellaceae and decreasing the abundance of Turicibacter, Escherichia-Shigella, Desulfovibrio, and Lachnospiraceae.
34355721	6	26	theme	TNF-α	844:848	arg1	levels					834:839	the expression levels	819:839	the expression levels of TNF-α and IL-6	819:857	BR14 significantly attenuated the expression levels of TNF-α and IL-6, while the expression of IL-10 was upregulated.
34355721	8	27	theme	gut	1261:1263	arg1	barrier					1265:1271	the gut barrier	1257:1271	the gut barrier	1257:1271	In summary, BR14 exhibited great potential in alleviating colitis through restoring the gut barrier and adjusting the intestinal microbiota.
34355721	8	28	theme	intestinal	1291:1300	arg1	microbiota					1302:1311	the intestinal microbiota	1287:1311	the intestinal microbiota	1287:1311	In summary, BR14 exhibited great potential in alleviating colitis through restoring the gut barrier and adjusting the intestinal microbiota.
34355721	0	29	theme	DSS-induced	33:43	arg1	colitis					45:51	DSS-induced colitis	33:51	DSS-induced colitis	33:51	Probiotic yeast BR14 ameliorates DSS-induced colitis by restoring the gut barrier and adjusting the intestinal microbiota.
34355721	4	30	theme	colitis	567:573	arg1	mice					575:578	the colitis mice	563:578	the colitis mice	563:578	As for the colitis mice, BR14 inhibited weight loss the most, as well as the disease activity index and colon shortening.
34355721	5	31	theme	expression	709:718	arg1	levels					720:725	the expression levels	705:725	the expression levels of genes related to histological damage	705:765	After treatment with BR14, the expression levels of genes related to histological damage were all upregulated.
34355721	7	32	theme	Desulfovibrio	1137:1149	arg1	abundance					1088:1096	the abundance	1084:1096	the abundance of Turicibacter, Escherichia-Shigella, Desulfovibrio, and Lachnospiraceae	1084:1170	Additionally, BR14 rebalanced the intestinal microbial composition of colitis mice by increasing the abundance of Muribaculaceae, Lactobacillus and Rikenellaceae and decreasing the abundance of Turicibacter, Escherichia-Shigella, Desulfovibrio, and Lachnospiraceae.
34355721	7	33	theme	Muribaculaceae	1021:1034	arg1	abundance					1008:1016	the abundance	1004:1016	the abundance of Muribaculaceae, Lactobacillus and Rikenellaceae	1004:1067	Additionally, BR14 rebalanced the intestinal microbial composition of colitis mice by increasing the abundance of Muribaculaceae, Lactobacillus and Rikenellaceae and decreasing the abundance of Turicibacter, Escherichia-Shigella, Desulfovibrio, and Lachnospiraceae.
34355721	2	34	theme	colitis	349:355	arg1	treatment					357:365	colitis treatment	349:365	colitis treatment	349:365	Saccharomyces cerevisiae with great stress tolerance and potential in colitis treatment was investigated in this study.
34355721	3	35	theme	yeast	408:412	arg1	strains					414:420	16 yeast strains	405:420	16 yeast strains	405:420	Among 16 yeast strains, BR14, BR54, and BR174 strains showed good stress-resistant capacity, anti-inflammatory activity, and little toxicity to macrophages.
34355721	6	36	theme	expression	823:832	arg1	levels					834:839	the expression levels	819:839	the expression levels of TNF-α and IL-6	819:857	BR14 significantly attenuated the expression levels of TNF-α and IL-6, while the expression of IL-10 was upregulated.
34355721	7	37	theme	mice	985:988	arg1	composition					962:972	the intestinal microbial composition	937:972	the intestinal microbial composition of colitis mice	937:988	Additionally, BR14 rebalanced the intestinal microbial composition of colitis mice by increasing the abundance of Muribaculaceae, Lactobacillus and Rikenellaceae and decreasing the abundance of Turicibacter, Escherichia-Shigella, Desulfovibrio, and Lachnospiraceae.
34355721	1	38	theme	probiotic	127:135	arg1	boulardii					151:159	The probiotic Saccharomyces boulardii	123:159	The probiotic Saccharomyces boulardii	123:159	The probiotic Saccharomyces boulardii has been widely used in colitis treatment; however, the beneficial effects of other yeast species are rarely studied.
34355721	4	39	theme	weight	596:601	arg1	loss					603:606	weight loss	596:606	weight loss	596:606	As for the colitis mice, BR14 inhibited weight loss the most, as well as the disease activity index and colon shortening.
34355721	1	40	theme	Saccharomyces	137:149	arg1	boulardii					151:159	The probiotic Saccharomyces boulardii	123:159	The probiotic Saccharomyces boulardii	123:159	The probiotic Saccharomyces boulardii has been widely used in colitis treatment; however, the beneficial effects of other yeast species are rarely studied.
34355721	3	41	theme	good	460:463	arg1	capacity					482:489	good stress-resistant capacity	460:489	good stress-resistant capacity	460:489	Among 16 yeast strains, BR14, BR54, and BR174 strains showed good stress-resistant capacity, anti-inflammatory activity, and little toxicity to macrophages.
34355721	0	42	theme	gut	70:72	arg1	barrier					74:80	the gut barrier	66:80	the gut barrier	66:80	Probiotic yeast BR14 ameliorates DSS-induced colitis by restoring the gut barrier and adjusting the intestinal microbiota.
34355721	7	43	theme	colitis	977:983	arg1	mice					985:988	colitis mice	977:988	colitis mice	977:988	Additionally, BR14 rebalanced the intestinal microbial composition of colitis mice by increasing the abundance of Muribaculaceae, Lactobacillus and Rikenellaceae and decreasing the abundance of Turicibacter, Escherichia-Shigella, Desulfovibrio, and Lachnospiraceae.
34355721	1	44	used	used	177:180	arg2	boulardii					151:159	The probiotic Saccharomyces boulardii	123:159	The probiotic Saccharomyces boulardii	123:159	The probiotic Saccharomyces boulardii has been widely used in colitis treatment; however, the beneficial effects of other yeast species are rarely studied.
34355721	7	45	theme	microbial	952:960	arg1	composition					962:972	the intestinal microbial composition	937:972	the intestinal microbial composition of colitis mice	937:988	Additionally, BR14 rebalanced the intestinal microbial composition of colitis mice by increasing the abundance of Muribaculaceae, Lactobacillus and Rikenellaceae and decreasing the abundance of Turicibacter, Escherichia-Shigella, Desulfovibrio, and Lachnospiraceae.
34355721	1	46	theme	beneficial	217:226	arg1	effects					228:234	the beneficial effects	213:234	the beneficial effects of other yeast species	213:257	The probiotic Saccharomyces boulardii has been widely used in colitis treatment; however, the beneficial effects of other yeast species are rarely studied.
34355721	1	47	theme	other	239:243	arg1	species					251:257	other yeast species	239:257	other yeast species	239:257	The probiotic Saccharomyces boulardii has been widely used in colitis treatment; however, the beneficial effects of other yeast species are rarely studied.
33340631	7	0	theme	deterioration	1122:1134	arg1	reactions					1136:1144	the deterioration reactions	1118:1144	the deterioration reactions	1118:1144	The active packaging containing zein nanoparticles was efficient in the conservation of ground beef, stabilizing the deterioration reactions and preserving the color.
33340631	2	1	theme	wall	374:377	arg1	material					379:386	wall material	374:386	wall material	374:386	In this study, the Cinnamodendron dinisii Schwanke essential oil was nanoencapsulated using zein as wall material, and applied in chitosan matrix to produce an active nanocomposite film packaging for food conservation.
33340631	1	2	theme	packaging	263:271	arg1	production					237:246	the production	233:246	the production of active food packaging	233:271	The nanoencapsulation of essential oils for biodegradable films functionalization is a viable alternative for the production of active food packaging.
33340631	2	3	theme	chitosan	404:411	arg1	matrix					413:418	chitosan matrix	404:418	chitosan matrix	404:418	In this study, the Cinnamodendron dinisii Schwanke essential oil was nanoencapsulated using zein as wall material, and applied in chitosan matrix to produce an active nanocomposite film packaging for food conservation.
33340631	6	4	theme	antimicrobial	981:993	arg1	activity					995:1002	antimicrobial activity	981:1002	antimicrobial activity	981:1002	The chitosan films obtained functionalized with nanoparticles demonstrated antioxidant activity and antimicrobial activity.
33340631	1	5	theme	biodegradable	167:179	arg1	functionalization					187:203	biodegradable films functionalization	167:203	biodegradable films functionalization	167:203	The nanoencapsulation of essential oils for biodegradable films functionalization is a viable alternative for the production of active food packaging.
33340631	2	6	theme	Cinnamodendron	293:306	arg1	oil					335:337	the Cinnamodendron dinisii Schwanke essential oil	289:337	the Cinnamodendron dinisii Schwanke essential oil	289:337	In this study, the Cinnamodendron dinisii Schwanke essential oil was nanoencapsulated using zein as wall material, and applied in chitosan matrix to produce an active nanocomposite film packaging for food conservation.
33340631	4	7	theme	lower	795:799	arg1	30 mV					764:768	30 mV	764:768	30 mV	764:768	The oil nanoencapsulation produced stable and homogeneous nanoparticles with zeta potential close to 30 mV and polydispersity index lower than 0.2.
33340631	7	8	theme	zein	1037:1040	arg1	nanoparticles					1042:1054	zein nanoparticles	1037:1054	zein nanoparticles	1037:1054	The active packaging containing zein nanoparticles was efficient in the conservation of ground beef, stabilizing the deterioration reactions and preserving the color.
33340631	4	9	theme	zeta	740:743	arg1	potential					745:753	zeta potential	740:753	zeta potential close to 30 mV and polydispersity index lower than 0.2	740:808	The oil nanoencapsulation produced stable and homogeneous nanoparticles with zeta potential close to 30 mV and polydispersity index lower than 0.2.
33340631	6	10	theme	antioxidant	956:966	arg1	activity					968:975	antioxidant activity	956:975	antioxidant activity	956:975	The chitosan films obtained functionalized with nanoparticles demonstrated antioxidant activity and antimicrobial activity.
33340631	3	11	theme	essential	557:565	arg1	oil					567:569	the Cinnamodendron dinisii Schwanke essential oil	521:569	the Cinnamodendron dinisii Schwanke essential oil	521:569	The chemical composition of the Cinnamodendron dinisii Schwanke essential oil showed a variety of unexplored bioactive compounds, and 1,8-cineole was the major compound.
33340631	7	12	from	conservation	1077:1088	arg1	packaging					1016:1024	The active packaging	1005:1024	The active packaging containing zein nanoparticles	1005:1054	The active packaging containing zein nanoparticles was efficient in the conservation of ground beef, stabilizing the deterioration reactions and preserving the color.
33340631	7	12	from	conservation	1077:1088	arg1	stabilizing					1106:1116	stabilizing	1106:1116	stabilizing the deterioration reactions	1106:1144	The active packaging containing zein nanoparticles was efficient in the conservation of ground beef, stabilizing the deterioration reactions and preserving the color.
33340631	7	12	from	conservation	1077:1088	arg1	preserving					1150:1159	preserving	1150:1159	preserving the color	1150:1169	The active packaging containing zein nanoparticles was efficient in the conservation of ground beef, stabilizing the deterioration reactions and preserving the color.
33340631	7	12	from	conservation	1077:1088	arg1	efficient					1060:1068	efficient	1060:1068	efficient	1060:1068	The active packaging containing zein nanoparticles was efficient in the conservation of ground beef, stabilizing the deterioration reactions and preserving the color.
33340631	1	13	theme	films	181:185	arg1	functionalization					187:203	biodegradable films functionalization	167:203	biodegradable films functionalization	167:203	The nanoencapsulation of essential oils for biodegradable films functionalization is a viable alternative for the production of active food packaging.
33340631	0	14	theme	meat	104:107	arg1	conservation					109:120	meat conservation	104:120	meat conservation	104:120	Chitosan packaging functionalized with Cinnamodendron dinisii essential oil loaded zein: A proposal for meat conservation.
33340631	0	15	theme	Chitosan	0:7	arg1	packaging					9:17	Chitosan packaging	0:17	Chitosan packaging	0:17	Chitosan packaging functionalized with Cinnamodendron dinisii essential oil loaded zein: A proposal for meat conservation.
33340631	3	16	theme	Schwanke	548:555	arg1	oil					567:569	the Cinnamodendron dinisii Schwanke essential oil	521:569	the Cinnamodendron dinisii Schwanke essential oil	521:569	The chemical composition of the Cinnamodendron dinisii Schwanke essential oil showed a variety of unexplored bioactive compounds, and 1,8-cineole was the major compound.
33340631	3	17	theme	unexplored	591:600	arg1	compounds					612:620	unexplored bioactive compounds	591:620	unexplored bioactive compounds	591:620	The chemical composition of the Cinnamodendron dinisii Schwanke essential oil showed a variety of unexplored bioactive compounds, and 1,8-cineole was the major compound.
33340631	4	18	theme	homogeneous	709:719	arg1	nanoparticles					721:733	stable and homogeneous nanoparticles	698:733	stable and homogeneous nanoparticles	698:733	The oil nanoencapsulation produced stable and homogeneous nanoparticles with zeta potential close to 30 mV and polydispersity index lower than 0.2.
33340631	2	19	theme	film	455:458	arg1	packaging					460:468	an active nanocomposite film packaging	431:468	an active nanocomposite film packaging for food conservation	431:490	In this study, the Cinnamodendron dinisii Schwanke essential oil was nanoencapsulated using zein as wall material, and applied in chitosan matrix to produce an active nanocomposite film packaging for food conservation.
33340631	4	20	theme	stable	698:703	arg1	nanoparticles					721:733	stable and homogeneous nanoparticles	698:733	stable and homogeneous nanoparticles	698:733	The oil nanoencapsulation produced stable and homogeneous nanoparticles with zeta potential close to 30 mV and polydispersity index lower than 0.2.
33340631	2	21	theme	essential	325:333	arg1	oil					335:337	the Cinnamodendron dinisii Schwanke essential oil	289:337	the Cinnamodendron dinisii Schwanke essential oil	289:337	In this study, the Cinnamodendron dinisii Schwanke essential oil was nanoencapsulated using zein as wall material, and applied in chitosan matrix to produce an active nanocomposite film packaging for food conservation.
33340631	2	22	theme	nanocomposite	441:453	arg1	packaging					460:468	an active nanocomposite film packaging	431:468	an active nanocomposite film packaging for food conservation	431:490	In this study, the Cinnamodendron dinisii Schwanke essential oil was nanoencapsulated using zein as wall material, and applied in chitosan matrix to produce an active nanocomposite film packaging for food conservation.
33340631	7	23	theme	active	1009:1014	arg1	packaging					1016:1024	The active packaging	1005:1024	The active packaging containing zein nanoparticles	1005:1054	The active packaging containing zein nanoparticles was efficient in the conservation of ground beef, stabilizing the deterioration reactions and preserving the color.
33340631	7	23	theme	active	1009:1014	arg1	efficient					1060:1068	efficient	1060:1068	efficient	1060:1068	The active packaging containing zein nanoparticles was efficient in the conservation of ground beef, stabilizing the deterioration reactions and preserving the color.
33340631	7	23	theme	active	1009:1014	arg1	preserving					1150:1159	preserving	1150:1159	preserving the color	1150:1169	The active packaging containing zein nanoparticles was efficient in the conservation of ground beef, stabilizing the deterioration reactions and preserving the color.
33340631	7	23	theme	active	1009:1014	arg1	stabilizing					1106:1116	stabilizing	1106:1116	stabilizing the deterioration reactions	1106:1144	The active packaging containing zein nanoparticles was efficient in the conservation of ground beef, stabilizing the deterioration reactions and preserving the color.
33340631	2	24	theme	Schwanke	316:323	arg1	oil					335:337	the Cinnamodendron dinisii Schwanke essential oil	289:337	the Cinnamodendron dinisii Schwanke essential oil	289:337	In this study, the Cinnamodendron dinisii Schwanke essential oil was nanoencapsulated using zein as wall material, and applied in chitosan matrix to produce an active nanocomposite film packaging for food conservation.
33340631	1	25	theme	viable	210:215	arg1	nanoencapsulation					127:143	The nanoencapsulation	123:143	The nanoencapsulation of essential oils for biodegradable films functionalization	123:203	The nanoencapsulation of essential oils for biodegradable films functionalization is a viable alternative for the production of active food packaging.
33340631	1	25	theme	viable	210:215	arg1	alternative					217:227	a viable alternative	208:227	a viable alternative for the production of active food packaging	208:271	The nanoencapsulation of essential oils for biodegradable films functionalization is a viable alternative for the production of active food packaging.
33340631	2	26	theme	active	434:439	arg1	packaging					460:468	an active nanocomposite film packaging	431:468	an active nanocomposite film packaging for food conservation	431:490	In this study, the Cinnamodendron dinisii Schwanke essential oil was nanoencapsulated using zein as wall material, and applied in chitosan matrix to produce an active nanocomposite film packaging for food conservation.
33340631	7	27	from	efficient	1060:1068	arg1	conservation					1077:1088	the conservation	1073:1088	the conservation of ground beef	1073:1103	The active packaging containing zein nanoparticles was efficient in the conservation of ground beef, stabilizing the deterioration reactions and preserving the color.
33340631	2	28	theme	dinisii	308:314	arg1	oil					335:337	the Cinnamodendron dinisii Schwanke essential oil	289:337	the Cinnamodendron dinisii Schwanke essential oil	289:337	In this study, the Cinnamodendron dinisii Schwanke essential oil was nanoencapsulated using zein as wall material, and applied in chitosan matrix to produce an active nanocomposite film packaging for food conservation.
33340631	3	29	theme	dinisii	540:546	arg1	oil					567:569	the Cinnamodendron dinisii Schwanke essential oil	521:569	the Cinnamodendron dinisii Schwanke essential oil	521:569	The chemical composition of the Cinnamodendron dinisii Schwanke essential oil showed a variety of unexplored bioactive compounds, and 1,8-cineole was the major compound.
33340631	0	30	theme	dinisii	54:60	arg1	oil					72:74	Cinnamodendron dinisii essential oil	39:74	Cinnamodendron dinisii essential oil loaded zein	39:86	Chitosan packaging functionalized with Cinnamodendron dinisii essential oil loaded zein: A proposal for meat conservation.
33340631	3	31	theme	bioactive	602:610	arg1	compounds					612:620	unexplored bioactive compounds	591:620	unexplored bioactive compounds	591:620	The chemical composition of the Cinnamodendron dinisii Schwanke essential oil showed a variety of unexplored bioactive compounds, and 1,8-cineole was the major compound.
33340631	0	32	theme	Cinnamodendron	39:52	arg1	oil					72:74	Cinnamodendron dinisii essential oil	39:74	Cinnamodendron dinisii essential oil loaded zein	39:86	Chitosan packaging functionalized with Cinnamodendron dinisii essential oil loaded zein: A proposal for meat conservation.
33340631	5	33	theme	nanoparticles	815:827	arg1	size					829:832	The nanoparticles size	811:832	The nanoparticles size	811:832	The nanoparticles size showed a size variation between 70 and 110 nm.
33340631	7	34	contain	containing	1026:1035	arg1	packaging					1016:1024	The active packaging	1005:1024	The active packaging containing zein nanoparticles	1005:1054	The active packaging containing zein nanoparticles was efficient in the conservation of ground beef, stabilizing the deterioration reactions and preserving the color.
33340631	7	34	contain	containing	1026:1035	arg2	nanoparticles					1042:1054	zein nanoparticles	1037:1054	zein nanoparticles	1037:1054	The active packaging containing zein nanoparticles was efficient in the conservation of ground beef, stabilizing the deterioration reactions and preserving the color.
33340631	7	34	contain	containing	1026:1035	arg1	efficient					1060:1068	efficient	1060:1068	efficient	1060:1068	The active packaging containing zein nanoparticles was efficient in the conservation of ground beef, stabilizing the deterioration reactions and preserving the color.
33340631	7	34	contain	containing	1026:1035	arg1	preserving					1150:1159	preserving	1150:1159	preserving the color	1150:1169	The active packaging containing zein nanoparticles was efficient in the conservation of ground beef, stabilizing the deterioration reactions and preserving the color.
33340631	7	34	contain	containing	1026:1035	arg1	stabilizing					1106:1116	stabilizing	1106:1116	stabilizing the deterioration reactions	1106:1144	The active packaging containing zein nanoparticles was efficient in the conservation of ground beef, stabilizing the deterioration reactions and preserving the color.
33340631	4	35	theme	oil	667:669	arg1	nanoencapsulation					671:687	The oil nanoencapsulation	663:687	The oil nanoencapsulation	663:687	The oil nanoencapsulation produced stable and homogeneous nanoparticles with zeta potential close to 30 mV and polydispersity index lower than 0.2.
33340631	2	36	theme	food	474:477	arg1	conservation					479:490	food conservation	474:490	food conservation	474:490	In this study, the Cinnamodendron dinisii Schwanke essential oil was nanoencapsulated using zein as wall material, and applied in chitosan matrix to produce an active nanocomposite film packaging for food conservation.
33340631	5	37	theme	size	843:846	arg1	variation					848:856	a size variation	841:856	a size variation between 70 and 110 nm	841:878	The nanoparticles size showed a size variation between 70 and 110 nm.
33340631	3	38	theme	compounds	612:620	arg1	compounds					612:620	unexplored bioactive compounds	591:620	unexplored bioactive compounds	591:620	The chemical composition of the Cinnamodendron dinisii Schwanke essential oil showed a variety of unexplored bioactive compounds, and 1,8-cineole was the major compound.
33340631	3	38	theme	compounds	612:620	arg1	variety					580:586	a variety	578:586	a variety of unexplored bioactive compounds	578:620	The chemical composition of the Cinnamodendron dinisii Schwanke essential oil showed a variety of unexplored bioactive compounds, and 1,8-cineole was the major compound.
33340631	0	39	theme	essential	62:70	arg1	oil					72:74	Cinnamodendron dinisii essential oil	39:74	Cinnamodendron dinisii essential oil loaded zein	39:86	Chitosan packaging functionalized with Cinnamodendron dinisii essential oil loaded zein: A proposal for meat conservation.
33340631	6	40	theme	chitosan	885:892	arg1	films					894:898	The chitosan films	881:898	The chitosan films obtained functionalized with nanoparticles	881:941	The chitosan films obtained functionalized with nanoparticles demonstrated antioxidant activity and antimicrobial activity.
33340631	3	41	theme	Cinnamodendron	525:538	arg1	oil					567:569	the Cinnamodendron dinisii Schwanke essential oil	521:569	the Cinnamodendron dinisii Schwanke essential oil	521:569	The chemical composition of the Cinnamodendron dinisii Schwanke essential oil showed a variety of unexplored bioactive compounds, and 1,8-cineole was the major compound.
33340631	3	42	theme	major	647:651	arg1	1,8-cineole					627:637	1,8-cineole	627:637	1,8-cineole	627:637	The chemical composition of the Cinnamodendron dinisii Schwanke essential oil showed a variety of unexplored bioactive compounds, and 1,8-cineole was the major compound.
33340631	3	42	theme	major	647:651	arg1	compound					653:660	the major compound	643:660	the major compound	643:660	The chemical composition of the Cinnamodendron dinisii Schwanke essential oil showed a variety of unexplored bioactive compounds, and 1,8-cineole was the major compound.
33340631	7	43	theme	beef	1100:1103	arg1	conservation					1077:1088	the conservation	1073:1088	the conservation of ground beef	1073:1103	The active packaging containing zein nanoparticles was efficient in the conservation of ground beef, stabilizing the deterioration reactions and preserving the color.
33340631	3	44	theme	oil	567:569	arg1	composition					506:516	The chemical composition	493:516	The chemical composition of the Cinnamodendron dinisii Schwanke essential oil	493:569	The chemical composition of the Cinnamodendron dinisii Schwanke essential oil showed a variety of unexplored bioactive compounds, and 1,8-cineole was the major compound.
33340631	0	45	dep	functionalized	19:32	arg1	proposal					91:98	A proposal	89:98	A proposal for meat conservation	89:120	Chitosan packaging functionalized with Cinnamodendron dinisii essential oil loaded zein: A proposal for meat conservation.
33340631	3	46	theme	chemical	497:504	arg1	composition					506:516	The chemical composition	493:516	The chemical composition of the Cinnamodendron dinisii Schwanke essential oil	493:569	The chemical composition of the Cinnamodendron dinisii Schwanke essential oil showed a variety of unexplored bioactive compounds, and 1,8-cineole was the major compound.
33340631	1	47	theme	essential	148:156	arg1	oils					158:161	essential oils	148:161	essential oils	148:161	The nanoencapsulation of essential oils for biodegradable films functionalization is a viable alternative for the production of active food packaging.
33340631	7	48	dep	efficient	1060:1068	arg1	packaging					1016:1024	The active packaging	1005:1024	The active packaging containing zein nanoparticles	1005:1054	The active packaging containing zein nanoparticles was efficient in the conservation of ground beef, stabilizing the deterioration reactions and preserving the color.
33340631	7	48	dep	efficient	1060:1068	arg1	efficient					1060:1068	efficient	1060:1068	efficient	1060:1068	The active packaging containing zein nanoparticles was efficient in the conservation of ground beef, stabilizing the deterioration reactions and preserving the color.
33340631	7	48	dep	efficient	1060:1068	arg1	preserving					1150:1159	preserving	1150:1159	preserving the color	1150:1169	The active packaging containing zein nanoparticles was efficient in the conservation of ground beef, stabilizing the deterioration reactions and preserving the color.
33340631	7	48	dep	efficient	1060:1068	arg1	stabilizing					1106:1116	stabilizing	1106:1116	stabilizing the deterioration reactions	1106:1144	The active packaging containing zein nanoparticles was efficient in the conservation of ground beef, stabilizing the deterioration reactions and preserving the color.
33340631	1	49	theme	active	251:256	arg1	packaging					263:271	active food packaging	251:271	active food packaging	251:271	The nanoencapsulation of essential oils for biodegradable films functionalization is a viable alternative for the production of active food packaging.
33340631	1	50	theme	oils	158:161	arg1	nanoencapsulation					127:143	The nanoencapsulation	123:143	The nanoencapsulation of essential oils for biodegradable films functionalization	123:203	The nanoencapsulation of essential oils for biodegradable films functionalization is a viable alternative for the production of active food packaging.
33340631	1	50	theme	oils	158:161	arg1	alternative					217:227	a viable alternative	208:227	a viable alternative for the production of active food packaging	208:271	The nanoencapsulation of essential oils for biodegradable films functionalization is a viable alternative for the production of active food packaging.
33340631	1	51	theme	food	258:261	arg1	packaging					263:271	active food packaging	251:271	active food packaging	251:271	The nanoencapsulation of essential oils for biodegradable films functionalization is a viable alternative for the production of active food packaging.
32944137	7	0	theme	phosphate-containing	1075:1094	arg1	inhibitors					1096:1105	other potent, but impermeable, phosphate-containing inhibitors	1044:1105	other potent, but impermeable, phosphate-containing inhibitors	1044:1105	It seems possible that other potent, but impermeable, phosphate-containing inhibitors might likewise benefit from this approach.
32944137	7	1	theme	other	1044:1048	arg1	inhibitors					1096:1105	other potent, but impermeable, phosphate-containing inhibitors	1044:1105	other potent, but impermeable, phosphate-containing inhibitors	1044:1105	It seems possible that other potent, but impermeable, phosphate-containing inhibitors might likewise benefit from this approach.
32944137	0	2	theme	Isomerase	101:109	arg1	Pin1					111:114	the Prolyl Isomerase Pin1	90:114	the Prolyl Isomerase Pin1	90:114	A Phosphoramidate Strategy Enables Membrane Permeability of a Non-nucleotide Inhibitor of the Prolyl Isomerase Pin1.
32944137	2	3	link	N-linked	321:328	arg1	ester					336:340	an N-linked amino ester	318:340	an N-linked amino ester	318:340	One strategy, termed "ProTide", masks the anionic phosphate through an N-linked amino ester and an O-linked aromatic phospho-ester, such that release of the active drug requires consecutive enzymatic liberation by an esterase and then a phosphoramidase, such as Hint1.
32944137	0	4	theme	Prolyl	94:99	arg1	Pin1					111:114	the Prolyl Isomerase Pin1	90:114	the Prolyl Isomerase Pin1	90:114	A Phosphoramidate Strategy Enables Membrane Permeability of a Non-nucleotide Inhibitor of the Prolyl Isomerase Pin1.
32944137	7	5	theme	impermeable	1062:1072	arg1	inhibitors					1096:1105	other potent, but impermeable, phosphate-containing inhibitors	1044:1105	other potent, but impermeable, phosphate-containing inhibitors	1044:1105	It seems possible that other potent, but impermeable, phosphate-containing inhibitors might likewise benefit from this approach.
32944137	5	6	theme	active	880:885	arg1	molecule					887:894	the active molecule	876:894	the active molecule to bind and inhibit Pin1 in cells	876:928	Moreover, this compound was processed by both esterase and phosphoramidase activity, releasing the active molecule to bind and inhibit Pin1 in cells.
32944137	7	7	theme	potent	1050:1055	arg1	inhibitors					1096:1105	other potent, but impermeable, phosphate-containing inhibitors	1044:1105	other potent, but impermeable, phosphate-containing inhibitors	1044:1105	It seems possible that other potent, but impermeable, phosphate-containing inhibitors might likewise benefit from this approach.
32944137	2	8	theme	ProTide	272:278	arg1	"					279:279	ProTide"	272:279	ProTide"	272:279	One strategy, termed "ProTide", masks the anionic phosphate through an N-linked amino ester and an O-linked aromatic phospho-ester, such that release of the active drug requires consecutive enzymatic liberation by an esterase and then a phosphoramidase, such as Hint1.
32944137	0	9	theme	Pin1	111:114	arg1	Inhibitor					77:85	a Non-nucleotide Inhibitor	60:85	a Non-nucleotide Inhibitor of the Prolyl Isomerase Pin1	60:114	A Phosphoramidate Strategy Enables Membrane Permeability of a Non-nucleotide Inhibitor of the Prolyl Isomerase Pin1.
32944137	2	10	link	O-linked	349:356	arg1	phospho-ester					367:379	an O-linked aromatic phospho-ester	346:379	an O-linked aromatic phospho-ester	346:379	One strategy, termed "ProTide", masks the anionic phosphate through an N-linked amino ester and an O-linked aromatic phospho-ester, such that release of the active drug requires consecutive enzymatic liberation by an esterase and then a phosphoramidase, such as Hint1.
32944137	4	11	theme	prolyl	731:736	arg1	Pin1					748:751	the prolyl isomerase Pin1	727:751	the prolyl isomerase Pin1	727:751	Here, we demonstrate that caging of a phosphate-containing inhibitor of the prolyl isomerase Pin1 increases its permeability.
32944137	2	12	theme	consecutive	428:438	arg1	liberation					450:459	consecutive enzymatic liberation	428:459	consecutive enzymatic liberation by an esterase	428:474	One strategy, termed "ProTide", masks the anionic phosphate through an N-linked amino ester and an O-linked aromatic phospho-ester, such that release of the active drug requires consecutive enzymatic liberation by an esterase and then a phosphoramidase, such as Hint1.
32944137	2	13	dep	masks	282:286	arg1	requires					419:426	requires	419:426	requires consecutive enzymatic liberation by an esterase	419:474	One strategy, termed "ProTide", masks the anionic phosphate through an N-linked amino ester and an O-linked aromatic phospho-ester, such that release of the active drug requires consecutive enzymatic liberation by an esterase and then a phosphoramidase, such as Hint1.
32944137	2	13	dep	masks	282:286	arg1	phosphoramidase					487:501	a phosphoramidase	485:501	a phosphoramidase	485:501	One strategy, termed "ProTide", masks the anionic phosphate through an N-linked amino ester and an O-linked aromatic phospho-ester, such that release of the active drug requires consecutive enzymatic liberation by an esterase and then a phosphoramidase, such as Hint1.
32944137	2	14	dep	requires	419:426	arg1	such					382:385	such	382:385	such	382:385	One strategy, termed "ProTide", masks the anionic phosphate through an N-linked amino ester and an O-linked aromatic phospho-ester, such that release of the active drug requires consecutive enzymatic liberation by an esterase and then a phosphoramidase, such as Hint1.
32944137	2	15	theme	anionic	292:298	arg1	phosphate					300:308	the anionic phosphate	288:308	the anionic phosphate	288:308	One strategy, termed "ProTide", masks the anionic phosphate through an N-linked amino ester and an O-linked aromatic phospho-ester, such that release of the active drug requires consecutive enzymatic liberation by an esterase and then a phosphoramidase, such as Hint1.
32944137	2	16	theme	aromatic	358:365	arg1	phospho-ester					367:379	an O-linked aromatic phospho-ester	346:379	an O-linked aromatic phospho-ester	346:379	One strategy, termed "ProTide", masks the anionic phosphate through an N-linked amino ester and an O-linked aromatic phospho-ester, such that release of the active drug requires consecutive enzymatic liberation by an esterase and then a phosphoramidase, such as Hint1.
32944137	2	17	theme	drug	414:417	arg1	release					392:398	release	392:398	release of the active drug	392:417	One strategy, termed "ProTide", masks the anionic phosphate through an N-linked amino ester and an O-linked aromatic phospho-ester, such that release of the active drug requires consecutive enzymatic liberation by an esterase and then a phosphoramidase, such as Hint1.
32944137	4	18	theme	inhibitor	714:722	arg1	caging					681:686	caging	681:686	caging of a phosphate-containing inhibitor of the prolyl isomerase Pin1	681:751	Here, we demonstrate that caging of a phosphate-containing inhibitor of the prolyl isomerase Pin1 increases its permeability.
32944137	2	19	theme	active	407:412	arg1	drug					414:417	the active drug	403:417	the active drug	403:417	One strategy, termed "ProTide", masks the anionic phosphate through an N-linked amino ester and an O-linked aromatic phospho-ester, such that release of the active drug requires consecutive enzymatic liberation by an esterase and then a phosphoramidase, such as Hint1.
32944137	4	20	theme	isomerase	738:746	arg1	Pin1					748:751	the prolyl isomerase Pin1	727:751	the prolyl isomerase Pin1	727:751	Here, we demonstrate that caging of a phosphate-containing inhibitor of the prolyl isomerase Pin1 increases its permeability.
32944137	3	21	theme	ProTide	599:605	arg1	approach					607:614	the ProTide approach	595:614	the ProTide approach	595:614	Because Hint1 is known to be selective for nucleotides, it was not clear if the ProTide approach could be deployed for non-nucleotides.
32944137	4	22	theme	phosphate-containing	693:712	arg1	inhibitor					714:722	a phosphate-containing inhibitor	691:722	a phosphate-containing inhibitor of the prolyl isomerase Pin1	691:751	Here, we demonstrate that caging of a phosphate-containing inhibitor of the prolyl isomerase Pin1 increases its permeability.
32944137	2	23	theme	O-linked	349:356	arg1	phospho-ester					367:379	an O-linked aromatic phospho-ester	346:379	an O-linked aromatic phospho-ester	346:379	One strategy, termed "ProTide", masks the anionic phosphate through an N-linked amino ester and an O-linked aromatic phospho-ester, such that release of the active drug requires consecutive enzymatic liberation by an esterase and then a phosphoramidase, such as Hint1.
32944137	2	24	theme	N-linked	321:328	arg1	ester					336:340	an N-linked amino ester	318:340	an N-linked amino ester	318:340	One strategy, termed "ProTide", masks the anionic phosphate through an N-linked amino ester and an O-linked aromatic phospho-ester, such that release of the active drug requires consecutive enzymatic liberation by an esterase and then a phosphoramidase, such as Hint1.
32944137	0	25	theme	Membrane	35:42	arg1	Permeability					44:55	Membrane Permeability	35:55	Membrane Permeability of a Non-nucleotide Inhibitor of the Prolyl Isomerase Pin1	35:114	A Phosphoramidate Strategy Enables Membrane Permeability of a Non-nucleotide Inhibitor of the Prolyl Isomerase Pin1.
32944137	6	26	theme	broader	966:972	arg1	substrates					981:990	substrates	981:990	substrates	981:990	Thus, Hint1 appears to recognize a broader set of substrates than previously appreciated.
32944137	6	26	theme	broader	966:972	arg1	set					974:976	a broader set	964:976	a broader set of substrates	964:990	Thus, Hint1 appears to recognize a broader set of substrates than previously appreciated.
32944137	6	27	theme	substrates	981:990	arg1	substrates					981:990	substrates	981:990	substrates	981:990	Thus, Hint1 appears to recognize a broader set of substrates than previously appreciated.
32944137	6	27	theme	substrates	981:990	arg1	set					974:976	a broader set	964:976	a broader set of substrates	964:990	Thus, Hint1 appears to recognize a broader set of substrates than previously appreciated.
32944137	2	28	theme	amino	330:334	arg1	ester					336:340	an N-linked amino ester	318:340	an N-linked amino ester	318:340	One strategy, termed "ProTide", masks the anionic phosphate through an N-linked amino ester and an O-linked aromatic phospho-ester, such that release of the active drug requires consecutive enzymatic liberation by an esterase and then a phosphoramidase, such as Hint1.
32944137	2	29	theme	enzymatic	440:448	arg1	liberation					450:459	consecutive enzymatic liberation	428:459	consecutive enzymatic liberation by an esterase	428:474	One strategy, termed "ProTide", masks the anionic phosphate through an N-linked amino ester and an O-linked aromatic phospho-ester, such that release of the active drug requires consecutive enzymatic liberation by an esterase and then a phosphoramidase, such as Hint1.
32944137	5	30	dep	esterase	827:834	arg1	activity					856:863	activity	856:863	activity	856:863	Moreover, this compound was processed by both esterase and phosphoramidase activity, releasing the active molecule to bind and inhibit Pin1 in cells.
32944137	1	31	theme	membrane	121:128	arg1	permeability					130:141	The membrane permeability	117:141	The membrane permeability of nucleotide-based drugs, such as sofosbuvir (Sovaldi),	117:198	The membrane permeability of nucleotide-based drugs, such as sofosbuvir (Sovaldi), requires installation of phosphate-caging groups.
32944137	5	32	from	Pin1	916:919	arg1	cells					924:928	cells	924:928	cells	924:928	Moreover, this compound was processed by both esterase and phosphoramidase activity, releasing the active molecule to bind and inhibit Pin1 in cells.
32944137	4	33	theme	Pin1	748:751	arg1	inhibitor					714:722	a phosphate-containing inhibitor	691:722	a phosphate-containing inhibitor of the prolyl isomerase Pin1	691:751	Here, we demonstrate that caging of a phosphate-containing inhibitor of the prolyl isomerase Pin1 increases its permeability.
32944137	1	34	theme	drugs	163:167	arg1	permeability					130:141	The membrane permeability	117:141	The membrane permeability of nucleotide-based drugs, such as sofosbuvir (Sovaldi),	117:198	The membrane permeability of nucleotide-based drugs, such as sofosbuvir (Sovaldi), requires installation of phosphate-caging groups.
32944137	1	35	theme	phosphate-caging	225:240	arg1	groups					242:247	phosphate-caging groups	225:247	phosphate-caging groups	225:247	The membrane permeability of nucleotide-based drugs, such as sofosbuvir (Sovaldi), requires installation of phosphate-caging groups.
32944137	0	36	theme	Inhibitor	77:85	arg1	Permeability					44:55	Membrane Permeability	35:55	Membrane Permeability of a Non-nucleotide Inhibitor of the Prolyl Isomerase Pin1	35:114	A Phosphoramidate Strategy Enables Membrane Permeability of a Non-nucleotide Inhibitor of the Prolyl Isomerase Pin1.
32944137	1	37	theme	nucleotide-based	146:161	arg1	drugs					163:167	nucleotide-based drugs	146:167	nucleotide-based drugs	146:167	The membrane permeability of nucleotide-based drugs, such as sofosbuvir (Sovaldi), requires installation of phosphate-caging groups.
32944137	1	37	theme	nucleotide-based	146:161	arg1	sofosbuvir					178:187	sofosbuvir	178:187	sofosbuvir (Sovaldi)	178:197	The membrane permeability of nucleotide-based drugs, such as sofosbuvir (Sovaldi), requires installation of phosphate-caging groups.
32944137	1	38	theme	groups	242:247	arg1	installation					209:220	installation	209:220	installation of phosphate-caging groups	209:247	The membrane permeability of nucleotide-based drugs, such as sofosbuvir (Sovaldi), requires installation of phosphate-caging groups.
32944137	0	39	theme	Non-nucleotide	62:75	arg1	Inhibitor					77:85	a Non-nucleotide Inhibitor	60:85	a Non-nucleotide Inhibitor of the Prolyl Isomerase Pin1	60:114	A Phosphoramidate Strategy Enables Membrane Permeability of a Non-nucleotide Inhibitor of the Prolyl Isomerase Pin1.
32944137	0	40	theme	Phosphoramidate	2:16	arg1	Strategy					18:25	A Phosphoramidate Strategy	0:25	A Phosphoramidate Strategy	0:25	A Phosphoramidate Strategy Enables Membrane Permeability of a Non-nucleotide Inhibitor of the Prolyl Isomerase Pin1.
32983120	10	0	from	bulls	1814:1818	arg1	spermatozoa					1794:1804	spermatozoa	1794:1804	spermatozoa from HF bulls	1794:1818	For example, after in vitro capacitation the mean phagocytosis rate (MPR) rate in spermatozoa from HF bulls significantly increased to 66.49% (P < 0.01).
32983120	10	0	from	bulls	1814:1818	arg1	rate					1786:1789	the mean phagocytosis rate (MPR) rate	1753:1789	the mean phagocytosis rate (MPR) rate in spermatozoa from HF bulls	1753:1818	For example, after in vitro capacitation the mean phagocytosis rate (MPR) rate in spermatozoa from HF bulls significantly increased to 66.49% (P < 0.01).
32983120	6	1	theme	fertile	1104:1110	arg1	bulls					1117:1121	high fertile (HF) bulls	1099:1121	high fertile (HF) bulls	1099:1121	Our results indicated that the spermatozoa from high fertile (HF) bulls possessed a higher abundance of O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine and N-linked glycans like [GlcNAc]1-3, N-acetylglucosamine than the low fertile (LF) bull spermatozoa.
32983120	3	2	theme	buffalo	679:685	arg1	bulls					687:691	buffalo bulls	679:691	buffalo bulls	679:691	This study aimed to implicate the sperm glycosylation pattern as one of the factors responsible for low conception rates observed in buffalo bulls.
32983120	1	3	theme	information-rich	233:248	arg1	frontier					250:257	an information-rich frontier	230:257	an information-rich frontier that is directly in contact with its immediate milieu	230:311	The glycans on the plasma membrane of cells manifest as the glycocalyx, which serves as an information-rich frontier that is directly in contact with its immediate milieu.
32983120	1	3	theme	information-rich	233:248	arg1	glycocalyx					202:211	the glycocalyx	198:211	the glycocalyx	198:211	The glycans on the plasma membrane of cells manifest as the glycocalyx, which serves as an information-rich frontier that is directly in contact with its immediate milieu.
32983120	6	4	theme	galactosyl	1178:1187	arg1	N-acetylgalactosamine					1196:1216	galactosyl (β-1,3)N-acetylgalactosamine	1178:1216	galactosyl (β-1,3)N-acetylgalactosamine	1178:1216	Our results indicated that the spermatozoa from high fertile (HF) bulls possessed a higher abundance of O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine and N-linked glycans like [GlcNAc]1-3, N-acetylglucosamine than the low fertile (LF) bull spermatozoa.
32983120	4	5	theme	immune	857:862	arg1	abilities					872:880	differential immune evasion abilities	844:880	differential immune evasion abilities	844:880	We hypothesized that a differential abundance of glycans exists on the spermatozoa from bulls of contrasting fertilizing abilities endowing them with differential immune evasion abilities.
32983120	11	6	theme	<	1907:1907	arg1	p					1905:1905	p < 0.01	1905:1912	p < 0.01	1905:1912	Likewise, the MPR increased to 70.63% (p < 0.01) after O-glycosidase & α2-3,6,8,9 Neuraminidase A treatment of spermatozoa from HF bulls.
32983120	11	6	theme	<	1907:1907	arg1	%					1902:1902	70.63%	1897:1902	70.63% (p < 0.01)	1897:1913	Likewise, the MPR increased to 70.63% (p < 0.01) after O-glycosidase & α2-3,6,8,9 Neuraminidase A treatment of spermatozoa from HF bulls.
32983120	1	7	with	contact	279:285	arg1	milieu					306:311	its immediate milieu	292:311	its immediate milieu	292:311	The glycans on the plasma membrane of cells manifest as the glycocalyx, which serves as an information-rich frontier that is directly in contact with its immediate milieu.
32983120	6	8	theme	O-linked	1155:1162	arg1	glycans					1164:1170	O-linked glycans	1155:1170	O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine	1155:1216	Our results indicated that the spermatozoa from high fertile (HF) bulls possessed a higher abundance of O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine and N-linked glycans like [GlcNAc]1-3, N-acetylglucosamine than the low fertile (LF) bull spermatozoa.
32983120	14	9	from	selection	2509:2517	arg1	techniques					2578:2587	the AI/IVF reproduction techniques	2554:2587	the AI/IVF reproduction techniques	2554:2587	Nevertheless, further studies are warranted to provide the measures to diagnose the sub-fertile phenotype thus preventing the losses incurred by incorrect selection of morphologically normal sperm in the AI/IVF reproduction techniques.
32983120	6	10	link	N-linked	1222:1229	arg1	glycans					1231:1237	N-linked glycans	1222:1237	N-linked glycans like [GlcNAc]1-3	1222:1254	Our results indicated that the spermatozoa from high fertile (HF) bulls possessed a higher abundance of O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine and N-linked glycans like [GlcNAc]1-3, N-acetylglucosamine than the low fertile (LF) bull spermatozoa.
32983120	11	11	theme	α2-3,6,8,9	1937:1946	arg1	treatment					1964:1972	O-glycosidase & α2-3,6,8,9 Neuraminidase A treatment	1921:1972	O-glycosidase & α2-3,6,8,9 Neuraminidase A treatment of spermatozoa from HF bulls	1921:2001	Likewise, the MPR increased to 70.63% (p < 0.01) after O-glycosidase & α2-3,6,8,9 Neuraminidase A treatment of spermatozoa from HF bulls.
32983120	1	12	theme	cells	180:184	arg1	membrane					168:175	the plasma membrane	157:175	the plasma membrane of cells	157:184	The glycans on the plasma membrane of cells manifest as the glycocalyx, which serves as an information-rich frontier that is directly in contact with its immediate milieu.
32983120	9	13	theme	sperm	1655:1659	arg1	arrangements					1668:1679	the inherent sperm glycan arrangements	1642:1679	the inherent sperm glycan arrangements	1642:1679	Furthermore, any introduced perturbations in the inherent sperm glycan arrangements promoted phagocytosis by PMNs.
32983120	10	14	theme	mean	1757:1760	arg1	rate					1786:1789	the mean phagocytosis rate (MPR) rate	1753:1789	the mean phagocytosis rate (MPR) rate in spermatozoa from HF bulls	1753:1818	For example, after in vitro capacitation the mean phagocytosis rate (MPR) rate in spermatozoa from HF bulls significantly increased to 66.49% (P < 0.01).
32983120	10	15	theme	in	1731:1732	arg1	capacitation					1740:1751	in vitro capacitation	1731:1751	in vitro capacitation	1731:1751	For example, after in vitro capacitation the mean phagocytosis rate (MPR) rate in spermatozoa from HF bulls significantly increased to 66.49% (P < 0.01).
32983120	11	16	theme	Neuraminidase	1948:1960	arg1	treatment					1964:1972	O-glycosidase & α2-3,6,8,9 Neuraminidase A treatment	1921:1972	O-glycosidase & α2-3,6,8,9 Neuraminidase A treatment of spermatozoa from HF bulls	1921:2001	Likewise, the MPR increased to 70.63% (p < 0.01) after O-glycosidase & α2-3,6,8,9 Neuraminidase A treatment of spermatozoa from HF bulls.
32983120	6	17	theme	bull	1303:1306	arg1	spermatozoa					1308:1318	the low fertile (LF) bull spermatozoa	1282:1318	the low fertile (LF) bull spermatozoa	1282:1318	Our results indicated that the spermatozoa from high fertile (HF) bulls possessed a higher abundance of O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine and N-linked glycans like [GlcNAc]1-3, N-acetylglucosamine than the low fertile (LF) bull spermatozoa.
32983120	10	18	theme	rate	1775:1778	arg1	rate					1786:1789	the mean phagocytosis rate (MPR) rate	1753:1789	the mean phagocytosis rate (MPR) rate in spermatozoa from HF bulls	1753:1818	For example, after in vitro capacitation the mean phagocytosis rate (MPR) rate in spermatozoa from HF bulls significantly increased to 66.49% (P < 0.01).
32983120	11	19	theme	O-glycosidase	1921:1933	arg1	treatment					1964:1972	O-glycosidase & α2-3,6,8,9 Neuraminidase A treatment	1921:1972	O-glycosidase & α2-3,6,8,9 Neuraminidase A treatment of spermatozoa from HF bulls	1921:2001	Likewise, the MPR increased to 70.63% (p < 0.01) after O-glycosidase & α2-3,6,8,9 Neuraminidase A treatment of spermatozoa from HF bulls.
32983120	11	20	theme	HF	1994:1995	arg1	bulls					1997:2001	HF bulls	1994:2001	HF bulls	1994:2001	Likewise, the MPR increased to 70.63% (p < 0.01) after O-glycosidase & α2-3,6,8,9 Neuraminidase A treatment of spermatozoa from HF bulls.
32983120	12	21	theme	<	2209:2209	arg1	P					2207:2207	P < 0.0001	2207:2216	P < 0.0001	2207:2216	Moreover, the percentage of PMNs forming neutrophil extracellular traps (NETs) was significantly higher, 41.47% when exposed to spermatozoa from LF bulls vis-à-vis the spermatozoa from HF bulls, 15.46% (P < 0.0001).
32983120	12	21	theme	<	2209:2209	arg1	%					2204:2204	15.46%	2199:2204	15.46% (P < 0.0001)	2199:2217	Moreover, the percentage of PMNs forming neutrophil extracellular traps (NETs) was significantly higher, 41.47% when exposed to spermatozoa from LF bulls vis-à-vis the spermatozoa from HF bulls, 15.46% (P < 0.0001).
32983120	6	22	theme	N-linked	1222:1229	arg1	glycans					1231:1237	N-linked glycans	1222:1237	N-linked glycans like [GlcNAc]1-3	1222:1254	Our results indicated that the spermatozoa from high fertile (HF) bulls possessed a higher abundance of O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine and N-linked glycans like [GlcNAc]1-3, N-acetylglucosamine than the low fertile (LF) bull spermatozoa.
32983120	3	23	theme	sperm	580:584	arg1	pattern					600:606	the sperm glycosylation pattern	576:606	the sperm glycosylation pattern	576:606	This study aimed to implicate the sperm glycosylation pattern as one of the factors responsible for low conception rates observed in buffalo bulls.
32983120	5	24	theme	abundance	929:937	arg1	role					914:917	the role	910:917	the role of glycan abundance in the phagocytosis and NETosis rates exhibited by female neutrophils (PMNs) upon exposure to such spermatozoa	910:1048	Therefore, we investigated the role of glycan abundance in the phagocytosis and NETosis rates exhibited by female neutrophils (PMNs) upon exposure to such spermatozoa.
32983120	11	25	theme	spermatozoa	1977:1987	arg1	treatment					1964:1972	O-glycosidase & α2-3,6,8,9 Neuraminidase A treatment	1921:1972	O-glycosidase & α2-3,6,8,9 Neuraminidase A treatment of spermatozoa from HF bulls	1921:2001	Likewise, the MPR increased to 70.63% (p < 0.01) after O-glycosidase & α2-3,6,8,9 Neuraminidase A treatment of spermatozoa from HF bulls.
32983120	6	26	dep	N-acetylgalactosamine	1196:1216	arg1	e.g.					1172:1175	e.g.	1172:1175	e.g.	1172:1175	Our results indicated that the spermatozoa from high fertile (HF) bulls possessed a higher abundance of O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine and N-linked glycans like [GlcNAc]1-3, N-acetylglucosamine than the low fertile (LF) bull spermatozoa.
32983120	6	27	theme	fertile	1290:1296	arg1	spermatozoa					1308:1318	the low fertile (LF) bull spermatozoa	1282:1318	the low fertile (LF) bull spermatozoa	1282:1318	Our results indicated that the spermatozoa from high fertile (HF) bulls possessed a higher abundance of O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine and N-linked glycans like [GlcNAc]1-3, N-acetylglucosamine than the low fertile (LF) bull spermatozoa.
32983120	7	28	theme	female	1429:1434	arg1	PMNs					1454:1457	PMNs	1454:1457	PMNs	1454:1457	This differential glycomic endowment appeared to affect the spermiophagy and NETosis rates exhibited by the female neutrophil cells (PMNs).
32983120	7	28	theme	female	1429:1434	arg1	cells					1447:1451	the female neutrophil cells	1425:1451	the female neutrophil cells (PMNs)	1425:1458	This differential glycomic endowment appeared to affect the spermiophagy and NETosis rates exhibited by the female neutrophil cells (PMNs).
32983120	12	29	dep	higher	2101:2106	arg1	%					2114:2114	41.47%	2109:2114	41.47% when exposed to spermatozoa from LF bulls vis-à-vis the spermatozoa from HF bulls	2109:2196	Moreover, the percentage of PMNs forming neutrophil extracellular traps (NETs) was significantly higher, 41.47% when exposed to spermatozoa from LF bulls vis-à-vis the spermatozoa from HF bulls, 15.46% (P < 0.0001).
32983120	12	29	dep	higher	2101:2106	arg1	%					2204:2204	15.46%	2199:2204	15.46% (P < 0.0001)	2199:2217	Moreover, the percentage of PMNs forming neutrophil extracellular traps (NETs) was significantly higher, 41.47% when exposed to spermatozoa from LF bulls vis-à-vis the spermatozoa from HF bulls, 15.46% (P < 0.0001).
32983120	4	30	theme	differential	717:728	arg1	abundance					730:738	a differential abundance	715:738	a differential abundance of glycans	715:749	We hypothesized that a differential abundance of glycans exists on the spermatozoa from bulls of contrasting fertilizing abilities endowing them with differential immune evasion abilities.
32983120	8	31	theme	mean	1465:1468	arg1	different					1521:1529	different	1521:1529	different	1521:1529	The mean percentage of phagocytizing PMNs was significantly different (P < 0.0001) for HF and LF bulls, 28.44 and 59.59%, respectively.
32983120	8	31	theme	mean	1465:1468	arg1	P					1532:1532	P < 0.0001	1532:1541	P < 0.0001	1532:1541	The mean percentage of phagocytizing PMNs was significantly different (P < 0.0001) for HF and LF bulls, 28.44 and 59.59%, respectively.
32983120	8	31	theme	mean	1465:1468	arg1	%					1580:1580	28.44 and 59.59%	1565:1580	28.44 and 59.59%	1565:1580	The mean percentage of phagocytizing PMNs was significantly different (P < 0.0001) for HF and LF bulls, 28.44 and 59.59%, respectively.
32983120	8	31	theme	mean	1465:1468	arg1	percentage					1470:1479	The mean percentage	1461:1479	The mean percentage of phagocytizing PMNs	1461:1501	The mean percentage of phagocytizing PMNs was significantly different (P < 0.0001) for HF and LF bulls, 28.44 and 59.59%, respectively.
32983120	7	32	theme	differential	1326:1337	arg1	endowment					1348:1356	This differential glycomic endowment	1321:1356	This differential glycomic endowment	1321:1356	This differential glycomic endowment appeared to affect the spermiophagy and NETosis rates exhibited by the female neutrophil cells (PMNs).
32983120	0	33	theme	Selective	49:57	arg1	Advantage					59:67	a Selective Advantage	47:67	a Selective Advantage to High Fertile Buffalo Spermatozoa for Immune-Evasion From Neutrophils	47:139	A Higher Abundance of O-Linked Glycans Confers a Selective Advantage to High Fertile Buffalo Spermatozoa for Immune-Evasion From Neutrophils.
32983120	10	34	theme	MPR	1781:1783	arg1	rate					1786:1789	the mean phagocytosis rate (MPR) rate	1753:1789	the mean phagocytosis rate (MPR) rate in spermatozoa from HF bulls	1753:1818	For example, after in vitro capacitation the mean phagocytosis rate (MPR) rate in spermatozoa from HF bulls significantly increased to 66.49% (P < 0.01).
32983120	2	35	theme	unique	455:460	arg1	functions					484:492	unique reproduction-specific functions	455:492	unique reproduction-specific functions e.g., inter-cellular recognition and communication	455:543	The glycoconjugates (GCs) that adorn most of the mammalian cells are also abundant in gametes, especially the spermatozoa where they perform unique reproduction-specific functions e.g., inter-cellular recognition and communication.
32983120	5	36	from	role	914:917	arg1	rates					971:975	the phagocytosis and NETosis rates	942:975	the phagocytosis and NETosis rates exhibited by female neutrophils (PMNs) upon exposure to such spermatozoa	942:1048	Therefore, we investigated the role of glycan abundance in the phagocytosis and NETosis rates exhibited by female neutrophils (PMNs) upon exposure to such spermatozoa.
32983120	8	37	theme	PMNs	1498:1501	arg1	different					1521:1529	different	1521:1529	different	1521:1529	The mean percentage of phagocytizing PMNs was significantly different (P < 0.0001) for HF and LF bulls, 28.44 and 59.59%, respectively.
32983120	8	37	theme	PMNs	1498:1501	arg1	P					1532:1532	P < 0.0001	1532:1541	P < 0.0001	1532:1541	The mean percentage of phagocytizing PMNs was significantly different (P < 0.0001) for HF and LF bulls, 28.44 and 59.59%, respectively.
32983120	8	37	theme	PMNs	1498:1501	arg1	%					1580:1580	28.44 and 59.59%	1565:1580	28.44 and 59.59%	1565:1580	The mean percentage of phagocytizing PMNs was significantly different (P < 0.0001) for HF and LF bulls, 28.44 and 59.59%, respectively.
32983120	8	37	theme	PMNs	1498:1501	arg1	percentage					1470:1479	The mean percentage	1461:1479	The mean percentage of phagocytizing PMNs	1461:1501	The mean percentage of phagocytizing PMNs was significantly different (P < 0.0001) for HF and LF bulls, 28.44 and 59.59%, respectively.
32983120	6	38	link	O-linked	1155:1162	arg1	glycans					1164:1170	O-linked glycans	1155:1170	O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine	1155:1216	Our results indicated that the spermatozoa from high fertile (HF) bulls possessed a higher abundance of O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine and N-linked glycans like [GlcNAc]1-3, N-acetylglucosamine than the low fertile (LF) bull spermatozoa.
32983120	0	39	theme	Fertile	77:83	arg1	Spermatozoa					93:103	High Fertile Buffalo Spermatozoa	72:103	High Fertile Buffalo Spermatozoa	72:103	A Higher Abundance of O-Linked Glycans Confers a Selective Advantage to High Fertile Buffalo Spermatozoa for Immune-Evasion From Neutrophils.
32983120	13	40	theme	glycans	2293:2299	arg1	role					2276:2279	the role	2272:2279	the role of O-linked glycans in the immune responses mounted against spermatozoa	2272:2351	This is a pioneer report specifically demonstrating the role of O-linked glycans in the immune responses mounted against spermatozoa.
32983120	2	41	theme	mammalian	363:371	arg1	cells					373:377	the mammalian cells	359:377	the mammalian cells	359:377	The glycoconjugates (GCs) that adorn most of the mammalian cells are also abundant in gametes, especially the spermatozoa where they perform unique reproduction-specific functions e.g., inter-cellular recognition and communication.
32983120	12	42	theme	bulls	2152:2156	arg1	vis-à-vis					2158:2166	LF bulls vis-à-vis	2149:2166	LF bulls vis-à-vis	2149:2166	Moreover, the percentage of PMNs forming neutrophil extracellular traps (NETs) was significantly higher, 41.47% when exposed to spermatozoa from LF bulls vis-à-vis the spermatozoa from HF bulls, 15.46% (P < 0.0001).
32983120	3	43	located	observed	667:674	arg2	rates					661:665	low conception rates	646:665	low conception rates observed in buffalo bulls	646:691	This study aimed to implicate the sperm glycosylation pattern as one of the factors responsible for low conception rates observed in buffalo bulls.
32983120	3	43	located	observed	667:674	arg1	bulls					687:691	buffalo bulls	679:691	buffalo bulls	679:691	This study aimed to implicate the sperm glycosylation pattern as one of the factors responsible for low conception rates observed in buffalo bulls.
32983120	3	44	theme	low	646:648	arg1	rates					661:665	low conception rates	646:665	low conception rates observed in buffalo bulls	646:691	This study aimed to implicate the sperm glycosylation pattern as one of the factors responsible for low conception rates observed in buffalo bulls.
32983120	8	45	theme	<	1534:1534	arg1	different					1521:1529	different	1521:1529	different	1521:1529	The mean percentage of phagocytizing PMNs was significantly different (P < 0.0001) for HF and LF bulls, 28.44 and 59.59%, respectively.
32983120	8	45	theme	<	1534:1534	arg1	percentage					1470:1479	The mean percentage	1461:1479	The mean percentage of phagocytizing PMNs	1461:1501	The mean percentage of phagocytizing PMNs was significantly different (P < 0.0001) for HF and LF bulls, 28.44 and 59.59%, respectively.
32983120	8	45	theme	<	1534:1534	arg1	%					1580:1580	28.44 and 59.59%	1565:1580	28.44 and 59.59%	1565:1580	The mean percentage of phagocytizing PMNs was significantly different (P < 0.0001) for HF and LF bulls, 28.44 and 59.59%, respectively.
32983120	8	45	theme	<	1534:1534	arg1	P					1532:1532	P < 0.0001	1532:1541	P < 0.0001	1532:1541	The mean percentage of phagocytizing PMNs was significantly different (P < 0.0001) for HF and LF bulls, 28.44 and 59.59%, respectively.
32983120	14	46	theme	sperm	2545:2549	arg1	selection					2509:2517	incorrect selection	2499:2517	incorrect selection of morphologically normal sperm in the AI/IVF reproduction techniques	2499:2587	Nevertheless, further studies are warranted to provide the measures to diagnose the sub-fertile phenotype thus preventing the losses incurred by incorrect selection of morphologically normal sperm in the AI/IVF reproduction techniques.
32983120	10	47	theme	HF	1811:1812	arg1	bulls					1814:1818	HF bulls	1811:1818	HF bulls	1811:1818	For example, after in vitro capacitation the mean phagocytosis rate (MPR) rate in spermatozoa from HF bulls significantly increased to 66.49% (P < 0.01).
32983120	6	48	dep	fertile	1104:1110	arg1	HF					1113:1114	HF	1113:1114	HF	1113:1114	Our results indicated that the spermatozoa from high fertile (HF) bulls possessed a higher abundance of O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine and N-linked glycans like [GlcNAc]1-3, N-acetylglucosamine than the low fertile (LF) bull spermatozoa.
32983120	0	49	theme	O-Linked	22:29	arg1	Glycans					31:37	O-Linked Glycans	22:37	O-Linked Glycans	22:37	A Higher Abundance of O-Linked Glycans Confers a Selective Advantage to High Fertile Buffalo Spermatozoa for Immune-Evasion From Neutrophils.
32983120	13	50	theme	pioneer	2230:2236	arg1	This					2220:2223	This	2220:2223	This	2220:2223	This is a pioneer report specifically demonstrating the role of O-linked glycans in the immune responses mounted against spermatozoa.
32983120	13	50	theme	pioneer	2230:2236	arg1	report					2238:2243	a pioneer report	2228:2243	a pioneer report specifically demonstrating the role of O-linked glycans in the immune responses mounted against spermatozoa	2228:2351	This is a pioneer report specifically demonstrating the role of O-linked glycans in the immune responses mounted against spermatozoa.
32983120	3	51	theme	responsible	630:640	arg1	factors					622:628	the factors	618:628	the factors responsible for low conception rates observed in buffalo bulls	618:691	This study aimed to implicate the sperm glycosylation pattern as one of the factors responsible for low conception rates observed in buffalo bulls.
32983120	12	52	from	bulls	2192:2196	arg1	spermatozoa					2132:2142	spermatozoa	2132:2142	spermatozoa from LF bulls vis-à-vis the spermatozoa from HF bulls	2132:2196	Moreover, the percentage of PMNs forming neutrophil extracellular traps (NETs) was significantly higher, 41.47% when exposed to spermatozoa from LF bulls vis-à-vis the spermatozoa from HF bulls, 15.46% (P < 0.0001).
32983120	1	53	theme	immediate	296:304	arg1	milieu					306:311	its immediate milieu	292:311	its immediate milieu	292:311	The glycans on the plasma membrane of cells manifest as the glycocalyx, which serves as an information-rich frontier that is directly in contact with its immediate milieu.
32983120	2	54	from	gametes	400:406	arg1	glycoconjugates					318:332	The glycoconjugates	314:332	The glycoconjugates (GCs) that adorn most of the mammalian cells	314:377	The glycoconjugates (GCs) that adorn most of the mammalian cells are also abundant in gametes, especially the spermatozoa where they perform unique reproduction-specific functions e.g., inter-cellular recognition and communication.
32983120	2	54	from	gametes	400:406	arg1	abundant					388:395	abundant	388:395	abundant	388:395	The glycoconjugates (GCs) that adorn most of the mammalian cells are also abundant in gametes, especially the spermatozoa where they perform unique reproduction-specific functions e.g., inter-cellular recognition and communication.
32983120	12	55	theme	extracellular	2056:2068	arg1	neutrophil					2045:2054	neutrophil extracellular traps	2045:2074	neutrophil extracellular traps (NETs)	2045:2081	Moreover, the percentage of PMNs forming neutrophil extracellular traps (NETs) was significantly higher, 41.47% when exposed to spermatozoa from LF bulls vis-à-vis the spermatozoa from HF bulls, 15.46% (P < 0.0001).
32983120	12	55	theme	extracellular	2056:2068	arg1	NETs					2077:2080	NETs	2077:2080	NETs	2077:2080	Moreover, the percentage of PMNs forming neutrophil extracellular traps (NETs) was significantly higher, 41.47% when exposed to spermatozoa from LF bulls vis-à-vis the spermatozoa from HF bulls, 15.46% (P < 0.0001).
32983120	14	56	theme	reproduction	2565:2576	arg1	techniques					2578:2587	the AI/IVF reproduction techniques	2554:2587	the AI/IVF reproduction techniques	2554:2587	Nevertheless, further studies are warranted to provide the measures to diagnose the sub-fertile phenotype thus preventing the losses incurred by incorrect selection of morphologically normal sperm in the AI/IVF reproduction techniques.
32983120	0	57	from	Neutrophils	129:139	arg1	Immune-Evasion					109:122	Immune-Evasion	109:122	Immune-Evasion	109:122	A Higher Abundance of O-Linked Glycans Confers a Selective Advantage to High Fertile Buffalo Spermatozoa for Immune-Evasion From Neutrophils.
32983120	4	58	theme	fertilizing	803:813	arg1	abilities					815:823	contrasting fertilizing abilities	791:823	contrasting fertilizing abilities endowing them with differential immune evasion abilities	791:880	We hypothesized that a differential abundance of glycans exists on the spermatozoa from bulls of contrasting fertilizing abilities endowing them with differential immune evasion abilities.
32983120	8	59	dep	different	1521:1529	arg1	different					1521:1529	different	1521:1529	different	1521:1529	The mean percentage of phagocytizing PMNs was significantly different (P < 0.0001) for HF and LF bulls, 28.44 and 59.59%, respectively.
32983120	8	59	dep	different	1521:1529	arg1	percentage					1470:1479	The mean percentage	1461:1479	The mean percentage of phagocytizing PMNs	1461:1501	The mean percentage of phagocytizing PMNs was significantly different (P < 0.0001) for HF and LF bulls, 28.44 and 59.59%, respectively.
32983120	8	59	dep	different	1521:1529	arg1	%					1580:1580	28.44 and 59.59%	1565:1580	28.44 and 59.59%	1565:1580	The mean percentage of phagocytizing PMNs was significantly different (P < 0.0001) for HF and LF bulls, 28.44 and 59.59%, respectively.
32983120	8	59	dep	different	1521:1529	arg1	P					1532:1532	P < 0.0001	1532:1541	P < 0.0001	1532:1541	The mean percentage of phagocytizing PMNs was significantly different (P < 0.0001) for HF and LF bulls, 28.44 and 59.59%, respectively.
32983120	2	60	from	abundant	388:395	arg1	spermatozoa					424:434	the spermatozoa	420:434	the spermatozoa where they perform unique reproduction-specific functions e.g., inter-cellular recognition and communication	420:543	The glycoconjugates (GCs) that adorn most of the mammalian cells are also abundant in gametes, especially the spermatozoa where they perform unique reproduction-specific functions e.g., inter-cellular recognition and communication.
32983120	2	60	from	abundant	388:395	arg1	gametes					400:406	gametes	400:406	gametes	400:406	The glycoconjugates (GCs) that adorn most of the mammalian cells are also abundant in gametes, especially the spermatozoa where they perform unique reproduction-specific functions e.g., inter-cellular recognition and communication.
32983120	10	61	theme	P	1855:1855	arg1	%					1852:1852	66.49%	1847:1852	66.49% (P < 0.01)	1847:1863	For example, after in vitro capacitation the mean phagocytosis rate (MPR) rate in spermatozoa from HF bulls significantly increased to 66.49% (P < 0.01).
32983120	10	61	theme	P	1855:1855	arg1	<					1857:1857	P < 0.01	1855:1862	P < 0.01	1855:1862	For example, after in vitro capacitation the mean phagocytosis rate (MPR) rate in spermatozoa from HF bulls significantly increased to 66.49% (P < 0.01).
32983120	6	62	theme	low	1286:1288	arg1	spermatozoa					1308:1318	the low fertile (LF) bull spermatozoa	1282:1318	the low fertile (LF) bull spermatozoa	1282:1318	Our results indicated that the spermatozoa from high fertile (HF) bulls possessed a higher abundance of O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine and N-linked glycans like [GlcNAc]1-3, N-acetylglucosamine than the low fertile (LF) bull spermatozoa.
32983120	6	63	theme	high	1099:1102	arg1	bulls					1117:1121	high fertile (HF) bulls	1099:1121	high fertile (HF) bulls	1099:1121	Our results indicated that the spermatozoa from high fertile (HF) bulls possessed a higher abundance of O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine and N-linked glycans like [GlcNAc]1-3, N-acetylglucosamine than the low fertile (LF) bull spermatozoa.
32983120	9	64	theme	introduced	1614:1623	arg1	perturbations					1625:1637	any introduced perturbations	1610:1637	any introduced perturbations in the inherent sperm glycan arrangements	1610:1679	Furthermore, any introduced perturbations in the inherent sperm glycan arrangements promoted phagocytosis by PMNs.
32983120	7	65	theme	neutrophil	1436:1445	arg1	PMNs					1454:1457	PMNs	1454:1457	PMNs	1454:1457	This differential glycomic endowment appeared to affect the spermiophagy and NETosis rates exhibited by the female neutrophil cells (PMNs).
32983120	7	65	theme	neutrophil	1436:1445	arg1	cells					1447:1451	the female neutrophil cells	1425:1451	the female neutrophil cells (PMNs)	1425:1458	This differential glycomic endowment appeared to affect the spermiophagy and NETosis rates exhibited by the female neutrophil cells (PMNs).
32983120	13	66	link	O-linked	2284:2291	arg1	glycans					2293:2299	O-linked glycans	2284:2299	O-linked glycans	2284:2299	This is a pioneer report specifically demonstrating the role of O-linked glycans in the immune responses mounted against spermatozoa.
32983120	1	67	theme	plasma	161:166	arg1	membrane					168:175	the plasma membrane	157:175	the plasma membrane of cells	157:184	The glycans on the plasma membrane of cells manifest as the glycocalyx, which serves as an information-rich frontier that is directly in contact with its immediate milieu.
32983120	4	68	theme	differential	844:855	arg1	abilities					872:880	differential immune evasion abilities	844:880	differential immune evasion abilities	844:880	We hypothesized that a differential abundance of glycans exists on the spermatozoa from bulls of contrasting fertilizing abilities endowing them with differential immune evasion abilities.
32983120	6	69	theme	glycans	1164:1170	arg1	abundance					1142:1150	a higher abundance	1133:1150	a higher abundance of O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine and N-linked glycans like [GlcNAc]1-3	1133:1254	Our results indicated that the spermatozoa from high fertile (HF) bulls possessed a higher abundance of O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine and N-linked glycans like [GlcNAc]1-3, N-acetylglucosamine than the low fertile (LF) bull spermatozoa.
32983120	6	69	theme	glycans	1164:1170	arg1	N-acetylglucosamine					1257:1275	N-acetylglucosamine	1257:1275	N-acetylglucosamine	1257:1275	Our results indicated that the spermatozoa from high fertile (HF) bulls possessed a higher abundance of O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine and N-linked glycans like [GlcNAc]1-3, N-acetylglucosamine than the low fertile (LF) bull spermatozoa.
32983120	11	70	theme	&	1935:1935	arg1	treatment					1964:1972	O-glycosidase & α2-3,6,8,9 Neuraminidase A treatment	1921:1972	O-glycosidase & α2-3,6,8,9 Neuraminidase A treatment of spermatozoa from HF bulls	1921:2001	Likewise, the MPR increased to 70.63% (p < 0.01) after O-glycosidase & α2-3,6,8,9 Neuraminidase A treatment of spermatozoa from HF bulls.
32983120	6	71	dep	fertile	1290:1296	arg1	LF					1299:1300	LF	1299:1300	LF	1299:1300	Our results indicated that the spermatozoa from high fertile (HF) bulls possessed a higher abundance of O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine and N-linked glycans like [GlcNAc]1-3, N-acetylglucosamine than the low fertile (LF) bull spermatozoa.
32983120	14	72	theme	normal	2538:2543	arg1	sperm					2545:2549	morphologically normal sperm	2522:2549	morphologically normal sperm	2522:2549	Nevertheless, further studies are warranted to provide the measures to diagnose the sub-fertile phenotype thus preventing the losses incurred by incorrect selection of morphologically normal sperm in the AI/IVF reproduction techniques.
32983120	4	73	theme	evasion	864:870	arg1	abilities					872:880	differential immune evasion abilities	844:880	differential immune evasion abilities	844:880	We hypothesized that a differential abundance of glycans exists on the spermatozoa from bulls of contrasting fertilizing abilities endowing them with differential immune evasion abilities.
32983120	12	74	theme	PMNs	2032:2035	arg1	higher					2101:2106	higher	2101:2106	higher	2101:2106	Moreover, the percentage of PMNs forming neutrophil extracellular traps (NETs) was significantly higher, 41.47% when exposed to spermatozoa from LF bulls vis-à-vis the spermatozoa from HF bulls, 15.46% (P < 0.0001).
32983120	12	74	theme	PMNs	2032:2035	arg1	percentage					2018:2027	the percentage	2014:2027	the percentage of PMNs forming neutrophil extracellular traps (NETs)	2014:2081	Moreover, the percentage of PMNs forming neutrophil extracellular traps (NETs) was significantly higher, 41.47% when exposed to spermatozoa from LF bulls vis-à-vis the spermatozoa from HF bulls, 15.46% (P < 0.0001).
32983120	9	75	from	perturbations	1625:1637	arg1	arrangements					1668:1679	the inherent sperm glycan arrangements	1642:1679	the inherent sperm glycan arrangements	1642:1679	Furthermore, any introduced perturbations in the inherent sperm glycan arrangements promoted phagocytosis by PMNs.
32983120	9	76	theme	glycan	1661:1666	arg1	arrangements					1668:1679	the inherent sperm glycan arrangements	1642:1679	the inherent sperm glycan arrangements	1642:1679	Furthermore, any introduced perturbations in the inherent sperm glycan arrangements promoted phagocytosis by PMNs.
32983120	6	77	theme	higher	1135:1140	arg1	abundance					1142:1150	a higher abundance	1133:1150	a higher abundance of O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine and N-linked glycans like [GlcNAc]1-3	1133:1254	Our results indicated that the spermatozoa from high fertile (HF) bulls possessed a higher abundance of O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine and N-linked glycans like [GlcNAc]1-3, N-acetylglucosamine than the low fertile (LF) bull spermatozoa.
32983120	6	77	theme	higher	1135:1140	arg1	N-acetylglucosamine					1257:1275	N-acetylglucosamine	1257:1275	N-acetylglucosamine	1257:1275	Our results indicated that the spermatozoa from high fertile (HF) bulls possessed a higher abundance of O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine and N-linked glycans like [GlcNAc]1-3, N-acetylglucosamine than the low fertile (LF) bull spermatozoa.
32983120	9	78	theme	inherent	1646:1653	arg1	arrangements					1668:1679	the inherent sperm glycan arrangements	1642:1679	the inherent sperm glycan arrangements	1642:1679	Furthermore, any introduced perturbations in the inherent sperm glycan arrangements promoted phagocytosis by PMNs.
32983120	3	79	theme	glycosylation	586:598	arg1	pattern					600:606	the sperm glycosylation pattern	576:606	the sperm glycosylation pattern	576:606	This study aimed to implicate the sperm glycosylation pattern as one of the factors responsible for low conception rates observed in buffalo bulls.
32983120	12	80	dep	neutrophil	2045:2054	arg1	traps					2070:2074	traps	2070:2074	traps	2070:2074	Moreover, the percentage of PMNs forming neutrophil extracellular traps (NETs) was significantly higher, 41.47% when exposed to spermatozoa from LF bulls vis-à-vis the spermatozoa from HF bulls, 15.46% (P < 0.0001).
32983120	14	81	theme	incorrect	2499:2507	arg1	selection					2509:2517	incorrect selection	2499:2517	incorrect selection of morphologically normal sperm in the AI/IVF reproduction techniques	2499:2587	Nevertheless, further studies are warranted to provide the measures to diagnose the sub-fertile phenotype thus preventing the losses incurred by incorrect selection of morphologically normal sperm in the AI/IVF reproduction techniques.
32983120	11	82	theme	A	1962:1962	arg1	treatment					1964:1972	O-glycosidase & α2-3,6,8,9 Neuraminidase A treatment	1921:1972	O-glycosidase & α2-3,6,8,9 Neuraminidase A treatment of spermatozoa from HF bulls	1921:2001	Likewise, the MPR increased to 70.63% (p < 0.01) after O-glycosidase & α2-3,6,8,9 Neuraminidase A treatment of spermatozoa from HF bulls.
32983120	6	83	contain	possessed	1123:1131	arg1	spermatozoa					1082:1092	the spermatozoa	1078:1092	the spermatozoa from high fertile (HF) bulls	1078:1121	Our results indicated that the spermatozoa from high fertile (HF) bulls possessed a higher abundance of O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine and N-linked glycans like [GlcNAc]1-3, N-acetylglucosamine than the low fertile (LF) bull spermatozoa.
32983120	6	83	contain	possessed	1123:1131	arg2	N-acetylglucosamine					1257:1275	N-acetylglucosamine	1257:1275	N-acetylglucosamine	1257:1275	Our results indicated that the spermatozoa from high fertile (HF) bulls possessed a higher abundance of O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine and N-linked glycans like [GlcNAc]1-3, N-acetylglucosamine than the low fertile (LF) bull spermatozoa.
32983120	6	83	contain	possessed	1123:1131	arg2	abundance					1142:1150	a higher abundance	1133:1150	a higher abundance of O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine and N-linked glycans like [GlcNAc]1-3	1133:1254	Our results indicated that the spermatozoa from high fertile (HF) bulls possessed a higher abundance of O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine and N-linked glycans like [GlcNAc]1-3, N-acetylglucosamine than the low fertile (LF) bull spermatozoa.
32983120	7	84	theme	spermiophagy	1381:1392	arg1	rates					1406:1410	the spermiophagy and NETosis rates	1377:1410	the spermiophagy and NETosis rates exhibited by the female neutrophil cells (PMNs)	1377:1458	This differential glycomic endowment appeared to affect the spermiophagy and NETosis rates exhibited by the female neutrophil cells (PMNs).
32983120	12	85	from	vis-à-vis	2158:2166	arg1	spermatozoa					2132:2142	spermatozoa	2132:2142	spermatozoa from LF bulls vis-à-vis the spermatozoa from HF bulls	2132:2196	Moreover, the percentage of PMNs forming neutrophil extracellular traps (NETs) was significantly higher, 41.47% when exposed to spermatozoa from LF bulls vis-à-vis the spermatozoa from HF bulls, 15.46% (P < 0.0001).
32983120	13	86	theme	immune	2308:2313	arg1	responses					2315:2323	the immune responses	2304:2323	the immune responses mounted against spermatozoa	2304:2351	This is a pioneer report specifically demonstrating the role of O-linked glycans in the immune responses mounted against spermatozoa.
32983120	8	87	dep	HF	1548:1549	arg1	bulls					1558:1562	bulls	1558:1562	bulls	1558:1562	The mean percentage of phagocytizing PMNs was significantly different (P < 0.0001) for HF and LF bulls, 28.44 and 59.59%, respectively.
32983120	10	88	theme	phagocytosis	1762:1773	arg1	rate					1786:1789	the mean phagocytosis rate (MPR) rate	1753:1789	the mean phagocytosis rate (MPR) rate in spermatozoa from HF bulls	1753:1818	For example, after in vitro capacitation the mean phagocytosis rate (MPR) rate in spermatozoa from HF bulls significantly increased to 66.49% (P < 0.01).
32983120	5	89	theme	glycan	922:927	arg1	abundance					929:937	glycan abundance	922:937	glycan abundance	922:937	Therefore, we investigated the role of glycan abundance in the phagocytosis and NETosis rates exhibited by female neutrophils (PMNs) upon exposure to such spermatozoa.
32983120	10	90	dep	in	1731:1732	arg1	vitro					1734:1738	vitro	1734:1738	vitro	1734:1738	For example, after in vitro capacitation the mean phagocytosis rate (MPR) rate in spermatozoa from HF bulls significantly increased to 66.49% (P < 0.01).
32983120	13	91	from	role	2276:2279	arg1	responses					2315:2323	the immune responses	2304:2323	the immune responses mounted against spermatozoa	2304:2351	This is a pioneer report specifically demonstrating the role of O-linked glycans in the immune responses mounted against spermatozoa.
32983120	11	92	from	treatment	1964:1972	arg1	bulls					1997:2001	HF bulls	1994:2001	HF bulls	1994:2001	Likewise, the MPR increased to 70.63% (p < 0.01) after O-glycosidase & α2-3,6,8,9 Neuraminidase A treatment of spermatozoa from HF bulls.
32983120	12	93	dep	spermatozoa	2132:2142	arg1	spermatozoa					2172:2182	the spermatozoa	2168:2182	spermatozoa from LF bulls vis-à-vis the spermatozoa from HF bulls	2132:2196	Moreover, the percentage of PMNs forming neutrophil extracellular traps (NETs) was significantly higher, 41.47% when exposed to spermatozoa from LF bulls vis-à-vis the spermatozoa from HF bulls, 15.46% (P < 0.0001).
32983120	2	94	theme	reproduction-specific	462:482	arg1	functions					484:492	unique reproduction-specific functions	455:492	unique reproduction-specific functions e.g., inter-cellular recognition and communication	455:543	The glycoconjugates (GCs) that adorn most of the mammalian cells are also abundant in gametes, especially the spermatozoa where they perform unique reproduction-specific functions e.g., inter-cellular recognition and communication.
32983120	8	95	theme	phagocytizing	1484:1496	arg1	PMNs					1498:1501	phagocytizing PMNs	1484:1501	phagocytizing PMNs	1484:1501	The mean percentage of phagocytizing PMNs was significantly different (P < 0.0001) for HF and LF bulls, 28.44 and 59.59%, respectively.
32983120	7	96	theme	glycomic	1339:1346	arg1	endowment					1348:1356	This differential glycomic endowment	1321:1356	This differential glycomic endowment	1321:1356	This differential glycomic endowment appeared to affect the spermiophagy and NETosis rates exhibited by the female neutrophil cells (PMNs).
32983120	11	97	from	bulls	1997:2001	arg1	treatment					1964:1972	O-glycosidase & α2-3,6,8,9 Neuraminidase A treatment	1921:1972	O-glycosidase & α2-3,6,8,9 Neuraminidase A treatment of spermatozoa from HF bulls	1921:2001	Likewise, the MPR increased to 70.63% (p < 0.01) after O-glycosidase & α2-3,6,8,9 Neuraminidase A treatment of spermatozoa from HF bulls.
32983120	11	97	from	bulls	1997:2001	arg1	spermatozoa					1977:1987	spermatozoa	1977:1987	spermatozoa from HF bulls	1977:2001	Likewise, the MPR increased to 70.63% (p < 0.01) after O-glycosidase & α2-3,6,8,9 Neuraminidase A treatment of spermatozoa from HF bulls.
32983120	0	98	theme	High	72:75	arg1	Spermatozoa					93:103	High Fertile Buffalo Spermatozoa	72:103	High Fertile Buffalo Spermatozoa	72:103	A Higher Abundance of O-Linked Glycans Confers a Selective Advantage to High Fertile Buffalo Spermatozoa for Immune-Evasion From Neutrophils.
32983120	4	99	from	bulls	782:786	arg1	spermatozoa					765:775	the spermatozoa	761:775	the spermatozoa from bulls of contrasting fertilizing abilities endowing them with differential immune evasion abilities	761:880	We hypothesized that a differential abundance of glycans exists on the spermatozoa from bulls of contrasting fertilizing abilities endowing them with differential immune evasion abilities.
32983120	14	100	theme	sub-fertile	2438:2448	arg1	phenotype					2450:2458	the sub-fertile phenotype	2434:2458	the sub-fertile phenotype	2434:2458	Nevertheless, further studies are warranted to provide the measures to diagnose the sub-fertile phenotype thus preventing the losses incurred by incorrect selection of morphologically normal sperm in the AI/IVF reproduction techniques.
32983120	12	101	theme	HF	2189:2190	arg1	bulls					2192:2196	HF bulls	2189:2196	HF bulls	2189:2196	Moreover, the percentage of PMNs forming neutrophil extracellular traps (NETs) was significantly higher, 41.47% when exposed to spermatozoa from LF bulls vis-à-vis the spermatozoa from HF bulls, 15.46% (P < 0.0001).
32983120	0	102	theme	Buffalo	85:91	arg1	Spermatozoa					93:103	High Fertile Buffalo Spermatozoa	72:103	High Fertile Buffalo Spermatozoa	72:103	A Higher Abundance of O-Linked Glycans Confers a Selective Advantage to High Fertile Buffalo Spermatozoa for Immune-Evasion From Neutrophils.
32983120	13	103	theme	O-linked	2284:2291	arg1	glycans					2293:2299	O-linked glycans	2284:2299	O-linked glycans	2284:2299	This is a pioneer report specifically demonstrating the role of O-linked glycans in the immune responses mounted against spermatozoa.
32983120	7	104	theme	NETosis	1398:1404	arg1	rates					1406:1410	the spermiophagy and NETosis rates	1377:1410	the spermiophagy and NETosis rates exhibited by the female neutrophil cells (PMNs)	1377:1458	This differential glycomic endowment appeared to affect the spermiophagy and NETosis rates exhibited by the female neutrophil cells (PMNs).
32983120	2	105	theme	inter-cellular	500:513	arg1	recognition					515:525	inter-cellular recognition	500:525	inter-cellular recognition	500:525	The glycoconjugates (GCs) that adorn most of the mammalian cells are also abundant in gametes, especially the spermatozoa where they perform unique reproduction-specific functions e.g., inter-cellular recognition and communication.
32983120	1	106	from	glycans	146:152	arg1	membrane					168:175	the plasma membrane	157:175	the plasma membrane of cells	157:184	The glycans on the plasma membrane of cells manifest as the glycocalyx, which serves as an information-rich frontier that is directly in contact with its immediate milieu.
32983120	5	107	theme	female	990:995	arg1	PMNs					1010:1013	PMNs	1010:1013	PMNs	1010:1013	Therefore, we investigated the role of glycan abundance in the phagocytosis and NETosis rates exhibited by female neutrophils (PMNs) upon exposure to such spermatozoa.
32983120	5	107	theme	female	990:995	arg1	neutrophils					997:1007	female neutrophils	990:1007	female neutrophils (PMNs)	990:1014	Therefore, we investigated the role of glycan abundance in the phagocytosis and NETosis rates exhibited by female neutrophils (PMNs) upon exposure to such spermatozoa.
32983120	0	108	theme	Higher	2:7	arg1	Abundance					9:17	A Higher Abundance	0:17	A Higher Abundance of O-Linked Glycans	0:37	A Higher Abundance of O-Linked Glycans Confers a Selective Advantage to High Fertile Buffalo Spermatozoa for Immune-Evasion From Neutrophils.
32983120	5	109	theme	phagocytosis	946:957	arg1	rates					971:975	the phagocytosis and NETosis rates	942:975	the phagocytosis and NETosis rates exhibited by female neutrophils (PMNs) upon exposure to such spermatozoa	942:1048	Therefore, we investigated the role of glycan abundance in the phagocytosis and NETosis rates exhibited by female neutrophils (PMNs) upon exposure to such spermatozoa.
32983120	5	110	theme	NETosis	963:969	arg1	rates					971:975	the phagocytosis and NETosis rates	942:975	the phagocytosis and NETosis rates exhibited by female neutrophils (PMNs) upon exposure to such spermatozoa	942:1048	Therefore, we investigated the role of glycan abundance in the phagocytosis and NETosis rates exhibited by female neutrophils (PMNs) upon exposure to such spermatozoa.
32983120	4	111	theme	glycans	743:749	arg1	abundance					730:738	a differential abundance	715:738	a differential abundance of glycans	715:749	We hypothesized that a differential abundance of glycans exists on the spermatozoa from bulls of contrasting fertilizing abilities endowing them with differential immune evasion abilities.
32983120	0	112	theme	Glycans	31:37	arg1	Abundance					9:17	A Higher Abundance	0:17	A Higher Abundance of O-Linked Glycans	0:37	A Higher Abundance of O-Linked Glycans Confers a Selective Advantage to High Fertile Buffalo Spermatozoa for Immune-Evasion From Neutrophils.
32983120	2	113	dep	recognition	515:525	arg1	e.g.					494:497	e.g.	494:497	e.g.	494:497	The glycoconjugates (GCs) that adorn most of the mammalian cells are also abundant in gametes, especially the spermatozoa where they perform unique reproduction-specific functions e.g., inter-cellular recognition and communication.
32983120	10	114	from	rate	1786:1789	arg1	spermatozoa					1794:1804	spermatozoa	1794:1804	spermatozoa from HF bulls	1794:1818	For example, after in vitro capacitation the mean phagocytosis rate (MPR) rate in spermatozoa from HF bulls significantly increased to 66.49% (P < 0.01).
32983120	10	114	from	rate	1786:1789	arg1	bulls					1814:1818	HF bulls	1811:1818	HF bulls	1811:1818	For example, after in vitro capacitation the mean phagocytosis rate (MPR) rate in spermatozoa from HF bulls significantly increased to 66.49% (P < 0.01).
32983120	3	115	theme	factors	622:628	arg1	one					611:613	one	611:613	one	611:613	This study aimed to implicate the sperm glycosylation pattern as one of the factors responsible for low conception rates observed in buffalo bulls.
32983120	3	115	theme	factors	622:628	arg1	factors					622:628	the factors	618:628	the factors responsible for low conception rates observed in buffalo bulls	618:691	This study aimed to implicate the sperm glycosylation pattern as one of the factors responsible for low conception rates observed in buffalo bulls.
32983120	4	116	theme	abilities	815:823	arg1	bulls					782:786	bulls	782:786	bulls of contrasting fertilizing abilities endowing them with differential immune evasion abilities	782:880	We hypothesized that a differential abundance of glycans exists on the spermatozoa from bulls of contrasting fertilizing abilities endowing them with differential immune evasion abilities.
32983120	6	117	from	bulls	1117:1121	arg1	spermatozoa					1082:1092	the spermatozoa	1078:1092	the spermatozoa from high fertile (HF) bulls	1078:1121	Our results indicated that the spermatozoa from high fertile (HF) bulls possessed a higher abundance of O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine and N-linked glycans like [GlcNAc]1-3, N-acetylglucosamine than the low fertile (LF) bull spermatozoa.
32983120	5	118	theme	such	1033:1036	arg1	spermatozoa					1038:1048	such spermatozoa	1033:1048	such spermatozoa	1033:1048	Therefore, we investigated the role of glycan abundance in the phagocytosis and NETosis rates exhibited by female neutrophils (PMNs) upon exposure to such spermatozoa.
32983120	6	119	theme	glycans	1231:1237	arg1	abundance					1142:1150	a higher abundance	1133:1150	a higher abundance of O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine and N-linked glycans like [GlcNAc]1-3	1133:1254	Our results indicated that the spermatozoa from high fertile (HF) bulls possessed a higher abundance of O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine and N-linked glycans like [GlcNAc]1-3, N-acetylglucosamine than the low fertile (LF) bull spermatozoa.
32983120	6	119	theme	glycans	1231:1237	arg1	N-acetylglucosamine					1257:1275	N-acetylglucosamine	1257:1275	N-acetylglucosamine	1257:1275	Our results indicated that the spermatozoa from high fertile (HF) bulls possessed a higher abundance of O-linked glycans e.g., galactosyl (β-1,3)N-acetylgalactosamine and N-linked glycans like [GlcNAc]1-3, N-acetylglucosamine than the low fertile (LF) bull spermatozoa.
32983120	3	120	theme	conception	650:659	arg1	rates					661:665	low conception rates	646:665	low conception rates observed in buffalo bulls	646:691	This study aimed to implicate the sperm glycosylation pattern as one of the factors responsible for low conception rates observed in buffalo bulls.
32983120	14	121	theme	further	2368:2374	arg1	studies					2376:2382	further studies	2368:2382	further studies	2368:2382	Nevertheless, further studies are warranted to provide the measures to diagnose the sub-fertile phenotype thus preventing the losses incurred by incorrect selection of morphologically normal sperm in the AI/IVF reproduction techniques.
32983120	14	122	theme	AI/IVF	2558:2563	arg1	techniques					2578:2587	the AI/IVF reproduction techniques	2554:2587	the AI/IVF reproduction techniques	2554:2587	Nevertheless, further studies are warranted to provide the measures to diagnose the sub-fertile phenotype thus preventing the losses incurred by incorrect selection of morphologically normal sperm in the AI/IVF reproduction techniques.
32983120	4	123	theme	contrasting	791:801	arg1	abilities					815:823	contrasting fertilizing abilities	791:823	contrasting fertilizing abilities endowing them with differential immune evasion abilities	791:880	We hypothesized that a differential abundance of glycans exists on the spermatozoa from bulls of contrasting fertilizing abilities endowing them with differential immune evasion abilities.
33758306	1	0	theme	immune	243:248	arg1	function					250:257	immune function	243:257	immune function	243:257	This study was conducted to investigate the relationship between different cornstarch levels in tilapia diet and immune function.
33758306	3	1	theme	mean	440:443	arg1	weight					450:455	initial mean body weight 31.73 ± 1.36 g	432:470	initial mean body weight 31.73 ± 1.36 g	432:470	Three hundred and sixty fish (initial mean body weight 31.73 ± 1.36 g) were randomly allocated into twelve water-circulated tanks, and thirty fish per tank.
33758306	3	1	theme	mean	440:443	arg1	fish					426:429	Three hundred and sixty fish	402:429	Three hundred and sixty fish (initial mean body weight 31.73 ± 1.36 g)	402:471	Three hundred and sixty fish (initial mean body weight 31.73 ± 1.36 g) were randomly allocated into twelve water-circulated tanks, and thirty fish per tank.
33758306	11	2	dep	significant	1777:1787	arg1	P > 0.05					1790:1797	P > 0.05	1790:1797	P > 0.05	1790:1797	The challenge assay results revealed that the mortality rate of HS diet was higher than those of LS and MS diets, but the difference was not significant (P > 0.05).
33758306	11	2	dep	significant	1777:1787	arg1	difference					1758:1767	the difference	1754:1767	the difference	1754:1767	The challenge assay results revealed that the mortality rate of HS diet was higher than those of LS and MS diets, but the difference was not significant (P > 0.05).
33758306	11	2	dep	significant	1777:1787	arg1	significant					1777:1787	significant	1777:1787	significant	1777:1787	The challenge assay results revealed that the mortality rate of HS diet was higher than those of LS and MS diets, but the difference was not significant (P > 0.05).
33758306	11	3	theme	LS	1733:1734	arg1	diets					1743:1747	LS and MS diets	1733:1747	LS and MS diets	1733:1747	The challenge assay results revealed that the mortality rate of HS diet was higher than those of LS and MS diets, but the difference was not significant (P > 0.05).
33758306	12	4	theme	%	1857:1857	arg1	diet					1870:1873	the 36% cornstarch diet	1851:1873	the 36% cornstarch diet	1851:1873	In conclusion, the overall results suggested that the 36% cornstarch diet reduced not only the growth performance, but also body immunity.
33758306	0	5	theme	GIFT	94:97	arg1	niloticus					119:127	GIFT tilapia Oreochromis niloticus	94:127	GIFT tilapia Oreochromis niloticus	94:127	Impact of high dietary cornstarch level on growth, antioxidant response, and immune status in GIFT tilapia Oreochromis niloticus.
33758306	13	6	theme	experimental	1951:1962	arg1	condition					1964:1972	this experimental condition	1946:1972	this experimental condition	1946:1972	Under this experimental condition, GIFT tilapia could tolerate 18% cornstarch, but not 36% cornstarch.
33758306	8	7	theme	T-AOC	1252:1256	arg1	higher					1288:1293	higher	1288:1293	higher	1288:1293	The results of antioxidant experiments demonstrated that the content of SOD and T-AOC in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	8	7	theme	T-AOC	1252:1256	arg1	content					1233:1239	the content	1229:1239	the content of SOD and T-AOC in MS diet	1229:1267	The results of antioxidant experiments demonstrated that the content of SOD and T-AOC in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	0	8	theme	Oreochromis	107:117	arg1	niloticus					119:127	GIFT tilapia Oreochromis niloticus	94:127	GIFT tilapia Oreochromis niloticus	94:127	Impact of high dietary cornstarch level on growth, antioxidant response, and immune status in GIFT tilapia Oreochromis niloticus.
33758306	3	9	theme	water-circulated	509:524	arg1	tanks					526:530	twelve water-circulated tanks	502:530	twelve water-circulated tanks	502:530	Three hundred and sixty fish (initial mean body weight 31.73 ± 1.36 g) were randomly allocated into twelve water-circulated tanks, and thirty fish per tank.
33758306	1	10	theme	cornstarch	205:214	arg1	levels					216:221	different cornstarch levels	195:221	different cornstarch levels in tilapia diet and immune function	195:257	This study was conducted to investigate the relationship between different cornstarch levels in tilapia diet and immune function.
33758306	8	11	theme	MS	1261:1262	arg1	diet					1264:1267	MS diet	1261:1267	MS diet	1261:1267	The results of antioxidant experiments demonstrated that the content of SOD and T-AOC in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	5	12	theme	cornstarch	770:779	arg1	diet					781:784	The high cornstarch diet	761:784	The high cornstarch diet	761:784	The high cornstarch diet significantly decreased the content of crude protein and increased the content of crude lipid in whole body composition (P < 0.05).
33758306	0	13	from	status	84:89	arg1	niloticus					119:127	GIFT tilapia Oreochromis niloticus	94:127	GIFT tilapia Oreochromis niloticus	94:127	Impact of high dietary cornstarch level on growth, antioxidant response, and immune status in GIFT tilapia Oreochromis niloticus.
33758306	4	14	theme	medium	585:590	arg1	diets					603:607	the low and medium cornstarch diets	573:607	diets	603:607	Compared with the low and medium cornstarch diets, the results of growth showed that the high cornstarch diet significantly decreased the FBW, WGR, and SGR, and increased the FCR of tilapia (P < 0.05).
33758306	7	15	theme	index	1047:1051	arg1	results					1018:1024	The results	1014:1024	The results of blood biochemical index	1014:1051	The results of blood biochemical index exhibited that the HS diet significantly increased the content of blood glucose, and liver/muscle glycogen (P < 0.05).
33758306	11	16	theme	mortality	1682:1690	arg1	higher					1712:1717	higher	1712:1717	higher	1712:1717	The challenge assay results revealed that the mortality rate of HS diet was higher than those of LS and MS diets, but the difference was not significant (P > 0.05).
33758306	11	16	theme	mortality	1682:1690	arg1	rate					1692:1695	the mortality rate	1678:1695	the mortality rate of HS diet	1678:1706	The challenge assay results revealed that the mortality rate of HS diet was higher than those of LS and MS diets, but the difference was not significant (P > 0.05).
33758306	12	17	theme	body	1925:1928	arg1	immunity					1930:1937	body immunity	1925:1937	body immunity	1925:1937	In conclusion, the overall results suggested that the 36% cornstarch diet reduced not only the growth performance, but also body immunity.
33758306	7	18	theme	blood	1029:1033	arg1	index					1047:1051	blood biochemical index	1029:1051	blood biochemical index	1029:1051	The results of blood biochemical index exhibited that the HS diet significantly increased the content of blood glucose, and liver/muscle glycogen (P < 0.05).
33758306	0	19	from	Impact	0:5	arg1	response					63:70	antioxidant response	51:70	antioxidant response	51:70	Impact of high dietary cornstarch level on growth, antioxidant response, and immune status in GIFT tilapia Oreochromis niloticus.
33758306	0	19	from	Impact	0:5	arg1	status					84:89	immune status	77:89	immune status	77:89	Impact of high dietary cornstarch level on growth, antioxidant response, and immune status in GIFT tilapia Oreochromis niloticus.
33758306	0	19	from	Impact	0:5	arg1	growth					43:48	growth	43:48	growth	43:48	Impact of high dietary cornstarch level on growth, antioxidant response, and immune status in GIFT tilapia Oreochromis niloticus.
33758306	4	20	theme	cornstarch	653:662	arg1	diet					664:667	the high cornstarch diet	644:667	the high cornstarch diet	644:667	Compared with the low and medium cornstarch diets, the results of growth showed that the high cornstarch diet significantly decreased the FBW, WGR, and SGR, and increased the FCR of tilapia (P < 0.05).
33758306	5	21	theme	protein	831:837	arg1	content					814:820	the content	810:820	the content of crude protein	810:837	The high cornstarch diet significantly decreased the content of crude protein and increased the content of crude lipid in whole body composition (P < 0.05).
33758306	12	22	theme	overall	1820:1826	arg1	results					1828:1834	the overall results	1816:1834	the overall results	1816:1834	In conclusion, the overall results suggested that the 36% cornstarch diet reduced not only the growth performance, but also body immunity.
33758306	12	23	theme	growth	1896:1901	arg1	performance					1903:1913	the growth performance	1892:1913	the growth performance	1892:1913	In conclusion, the overall results suggested that the 36% cornstarch diet reduced not only the growth performance, but also body immunity.
33758306	0	24	theme	antioxidant	51:61	arg1	response					63:70	antioxidant response	51:70	antioxidant response	51:70	Impact of high dietary cornstarch level on growth, antioxidant response, and immune status in GIFT tilapia Oreochromis niloticus.
33758306	12	25	theme	cornstarch	1859:1868	arg1	diet					1870:1873	the 36% cornstarch diet	1851:1873	the 36% cornstarch diet	1851:1873	In conclusion, the overall results suggested that the 36% cornstarch diet reduced not only the growth performance, but also body immunity.
33758306	9	26	theme	MDA	1355:1357	arg1	lower					1388:1392	lower	1388:1392	lower	1388:1392	Meanwhile, the content of MDA in MS diet was significantly lower than that of HS diet (P < 0.05).
33758306	9	26	theme	MDA	1355:1357	arg1	content					1344:1350	the content	1340:1350	the content of MDA in MS diet	1340:1368	Meanwhile, the content of MDA in MS diet was significantly lower than that of HS diet (P < 0.05).
33758306	8	27	theme	antioxidant	1187:1197	arg1	experiments					1199:1209	antioxidant experiments	1187:1209	antioxidant experiments	1187:1209	The results of antioxidant experiments demonstrated that the content of SOD and T-AOC in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	0	28	theme	high	10:13	arg1	level					34:38	high dietary cornstarch level	10:38	high dietary cornstarch level	10:38	Impact of high dietary cornstarch level on growth, antioxidant response, and immune status in GIFT tilapia Oreochromis niloticus.
33758306	2	29	theme	test	264:267	arg1	fish					269:272	All test fish	260:272	All test fish	260:272	All test fish were fed with three cornstarch levels: low-cornstarch (0, LS), medium-cornstarch (18%, MS) and high-cornstarch (36%, HS) diets.
33758306	9	30	theme	MS	1362:1363	arg1	diet					1365:1368	MS diet	1362:1368	MS diet	1362:1368	Meanwhile, the content of MDA in MS diet was significantly lower than that of HS diet (P < 0.05).
33758306	7	31	theme	glucose	1125:1131	arg1	content					1108:1114	the content	1104:1114	the content of blood glucose	1104:1131	The results of blood biochemical index exhibited that the HS diet significantly increased the content of blood glucose, and liver/muscle glycogen (P < 0.05).
33758306	7	31	theme	glucose	1125:1131	arg1	P < 0.05					1161:1168	P < 0.05	1161:1168	P < 0.05	1161:1168	The results of blood biochemical index exhibited that the HS diet significantly increased the content of blood glucose, and liver/muscle glycogen (P < 0.05).
33758306	7	31	theme	glucose	1125:1131	arg1	glycogen					1151:1158	liver/muscle glycogen	1138:1158	liver/muscle glycogen (P < 0.05)	1138:1169	The results of blood biochemical index exhibited that the HS diet significantly increased the content of blood glucose, and liver/muscle glycogen (P < 0.05).
33758306	0	32	theme	cornstarch	23:32	arg1	level					34:38	high dietary cornstarch level	10:38	high dietary cornstarch level	10:38	Impact of high dietary cornstarch level on growth, antioxidant response, and immune status in GIFT tilapia Oreochromis niloticus.
33758306	10	33	theme	MS	1568:1569	arg1	diet					1571:1574	MS diet	1568:1574	MS diet	1568:1574	The results of immune index test showed that the lysozyme activities in the serum, liver, and gill, and the phagocytic activity and index in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	12	34	theme	36	1855:1856	arg1	%					1857:1857	%	1857:1857	%	1857:1857	In conclusion, the overall results suggested that the 36% cornstarch diet reduced not only the growth performance, but also body immunity.
33758306	5	35	theme	body	889:892	arg1	P < 0.05					907:914	P < 0.05	907:914	P < 0.05	907:914	The high cornstarch diet significantly decreased the content of crude protein and increased the content of crude lipid in whole body composition (P < 0.05).
33758306	5	35	theme	body	889:892	arg1	composition					894:904	whole body composition	883:904	whole body composition (P < 0.05)	883:915	The high cornstarch diet significantly decreased the content of crude protein and increased the content of crude lipid in whole body composition (P < 0.05).
33758306	2	36	dep	medium-cornstarch	337:353	arg1	MS					361:362	MS	361:362	MS	361:362	All test fish were fed with three cornstarch levels: low-cornstarch (0, LS), medium-cornstarch (18%, MS) and high-cornstarch (36%, HS) diets.
33758306	2	36	dep	medium-cornstarch	337:353	arg1	%					358:358	18%	356:358	18%	356:358	All test fish were fed with three cornstarch levels: low-cornstarch (0, LS), medium-cornstarch (18%, MS) and high-cornstarch (36%, HS) diets.
33758306	6	37	dep	VSI	932:934	arg1	the					928:930	the	928:930	the	928:930	Moreover, the VSI and CF in HS diet were significantly higher than those of LS diet (P < 0.05).
33758306	10	38	theme	index	1449:1453	arg1	test					1455:1458	immune index test	1442:1458	immune index test	1442:1458	The results of immune index test showed that the lysozyme activities in the serum, liver, and gill, and the phagocytic activity and index in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	11	39	theme	diet	1703:1706	arg1	higher					1712:1717	higher	1712:1717	higher	1712:1717	The challenge assay results revealed that the mortality rate of HS diet was higher than those of LS and MS diets, but the difference was not significant (P > 0.05).
33758306	11	39	theme	diet	1703:1706	arg1	rate					1692:1695	the mortality rate	1678:1695	the mortality rate of HS diet	1678:1706	The challenge assay results revealed that the mortality rate of HS diet was higher than those of LS and MS diets, but the difference was not significant (P > 0.05).
33758306	13	40	theme	%	2005:2005	arg1	cornstarch					2007:2016	18% cornstarch	2003:2016	18% cornstarch	2003:2016	Under this experimental condition, GIFT tilapia could tolerate 18% cornstarch, but not 36% cornstarch.
33758306	6	41	theme	HS	946:947	arg1	diet					949:952	HS diet	946:952	HS diet	946:952	Moreover, the VSI and CF in HS diet were significantly higher than those of LS diet (P < 0.05).
33758306	6	42	from	VSI	932:934	arg1	diet					949:952	HS diet	946:952	HS diet	946:952	Moreover, the VSI and CF in HS diet were significantly higher than those of LS diet (P < 0.05).
33758306	1	43	theme	tilapia	226:232	arg1	diet					234:237	tilapia diet	226:237	tilapia diet	226:237	This study was conducted to investigate the relationship between different cornstarch levels in tilapia diet and immune function.
33758306	5	44	theme	crude	868:872	arg1	lipid					874:878	crude lipid	868:878	crude lipid	868:878	The high cornstarch diet significantly decreased the content of crude protein and increased the content of crude lipid in whole body composition (P < 0.05).
33758306	7	45	theme	HS	1072:1073	arg1	diet					1075:1078	the HS diet	1068:1078	the HS diet	1068:1078	The results of blood biochemical index exhibited that the HS diet significantly increased the content of blood glucose, and liver/muscle glycogen (P < 0.05).
33758306	6	46	from	CF	940:941	arg1	diet					949:952	HS diet	946:952	HS diet	946:952	Moreover, the VSI and CF in HS diet were significantly higher than those of LS diet (P < 0.05).
33758306	10	47	theme	HS	1616:1617	arg1	P < 0.05					1625:1632	P < 0.05	1625:1632	P < 0.05	1625:1632	The results of immune index test showed that the lysozyme activities in the serum, liver, and gill, and the phagocytic activity and index in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	10	47	theme	HS	1616:1617	arg1	diet					1619:1622	HS diet	1616:1622	HS diet (P < 0.05)	1616:1633	The results of immune index test showed that the lysozyme activities in the serum, liver, and gill, and the phagocytic activity and index in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	13	48	theme	36	2027:2028	arg1	%					2029:2029	%	2029:2029	%	2029:2029	Under this experimental condition, GIFT tilapia could tolerate 18% cornstarch, but not 36% cornstarch.
33758306	3	49	theme	body	445:448	arg1	weight					450:455	initial mean body weight 31.73 ± 1.36 g	432:470	initial mean body weight 31.73 ± 1.36 g	432:470	Three hundred and sixty fish (initial mean body weight 31.73 ± 1.36 g) were randomly allocated into twelve water-circulated tanks, and thirty fish per tank.
33758306	3	49	theme	body	445:448	arg1	fish					426:429	Three hundred and sixty fish	402:429	Three hundred and sixty fish (initial mean body weight 31.73 ± 1.36 g)	402:471	Three hundred and sixty fish (initial mean body weight 31.73 ± 1.36 g) were randomly allocated into twelve water-circulated tanks, and thirty fish per tank.
33758306	4	50	theme	tilapia	741:747	arg1	FCR					734:736	the FCR	730:736	the FCR of tilapia (P < 0.05)	730:758	Compared with the low and medium cornstarch diets, the results of growth showed that the high cornstarch diet significantly decreased the FBW, WGR, and SGR, and increased the FCR of tilapia (P < 0.05).
33758306	11	51	theme	HS	1700:1701	arg1	diet					1703:1706	HS diet	1700:1706	HS diet	1700:1706	The challenge assay results revealed that the mortality rate of HS diet was higher than those of LS and MS diets, but the difference was not significant (P > 0.05).
33758306	13	52	theme	GIFT	1975:1978	arg1	tilapia					1980:1986	GIFT tilapia	1975:1986	GIFT tilapia	1975:1986	Under this experimental condition, GIFT tilapia could tolerate 18% cornstarch, but not 36% cornstarch.
33758306	6	53	theme	LS	994:995	arg1	diet					997:1000	LS diet	994:1000	LS diet (P < 0.05)	994:1011	Moreover, the VSI and CF in HS diet were significantly higher than those of LS diet (P < 0.05).
33758306	6	53	theme	LS	994:995	arg1	P < 0.05					1003:1010	P < 0.05	1003:1010	P < 0.05	1003:1010	Moreover, the VSI and CF in HS diet were significantly higher than those of LS diet (P < 0.05).
33758306	10	54	dep	activity	1546:1553	arg1	the					1531:1533	the	1531:1533	the	1531:1533	The results of immune index test showed that the lysozyme activities in the serum, liver, and gill, and the phagocytic activity and index in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	2	55	theme	cornstarch	294:303	arg1	levels					305:310	three cornstarch levels	288:310	three cornstarch levels	288:310	All test fish were fed with three cornstarch levels: low-cornstarch (0, LS), medium-cornstarch (18%, MS) and high-cornstarch (36%, HS) diets.
33758306	5	56	from	content	857:863	arg1	P < 0.05					907:914	P < 0.05	907:914	P < 0.05	907:914	The high cornstarch diet significantly decreased the content of crude protein and increased the content of crude lipid in whole body composition (P < 0.05).
33758306	5	56	from	content	857:863	arg1	composition					894:904	whole body composition	883:904	whole body composition (P < 0.05)	883:915	The high cornstarch diet significantly decreased the content of crude protein and increased the content of crude lipid in whole body composition (P < 0.05).
33758306	10	57	from	activities	1485:1494	arg1	serum					1503:1507	serum	1503:1507	serum	1503:1507	The results of immune index test showed that the lysozyme activities in the serum, liver, and gill, and the phagocytic activity and index in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	10	57	from	activities	1485:1494	arg1	diet					1571:1574	MS diet	1568:1574	MS diet	1568:1574	The results of immune index test showed that the lysozyme activities in the serum, liver, and gill, and the phagocytic activity and index in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	10	57	from	activities	1485:1494	arg1	liver					1510:1514	liver	1510:1514	liver	1510:1514	The results of immune index test showed that the lysozyme activities in the serum, liver, and gill, and the phagocytic activity and index in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	10	57	from	activities	1485:1494	arg1	gill					1521:1524	gill	1521:1524	gill	1521:1524	The results of immune index test showed that the lysozyme activities in the serum, liver, and gill, and the phagocytic activity and index in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	0	58	theme	tilapia	99:105	arg1	niloticus					119:127	GIFT tilapia Oreochromis niloticus	94:127	GIFT tilapia Oreochromis niloticus	94:127	Impact of high dietary cornstarch level on growth, antioxidant response, and immune status in GIFT tilapia Oreochromis niloticus.
33758306	13	59	theme	%	2029:2029	arg1	cornstarch					2031:2040	36% cornstarch	2027:2040	36% cornstarch	2027:2040	Under this experimental condition, GIFT tilapia could tolerate 18% cornstarch, but not 36% cornstarch.
33758306	10	60	dep	serum	1503:1507	arg1	the					1499:1501	the	1499:1501	the	1499:1501	The results of immune index test showed that the lysozyme activities in the serum, liver, and gill, and the phagocytic activity and index in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	10	61	theme	phagocytic	1535:1544	arg1	activity					1546:1553	phagocytic activity	1535:1553	phagocytic activity	1535:1553	The results of immune index test showed that the lysozyme activities in the serum, liver, and gill, and the phagocytic activity and index in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	8	62	from	content	1233:1239	arg1	diet					1264:1267	MS diet	1261:1267	MS diet	1261:1267	The results of antioxidant experiments demonstrated that the content of SOD and T-AOC in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	1	63	theme	different	195:203	arg1	levels					216:221	different cornstarch levels	195:221	different cornstarch levels in tilapia diet and immune function	195:257	This study was conducted to investigate the relationship between different cornstarch levels in tilapia diet and immune function.
33758306	11	64	theme	MS	1740:1741	arg1	diets					1743:1747	LS and MS diets	1733:1747	LS and MS diets	1733:1747	The challenge assay results revealed that the mortality rate of HS diet was higher than those of LS and MS diets, but the difference was not significant (P > 0.05).
33758306	11	65	theme	assay	1650:1654	arg1	results					1656:1662	The challenge assay results	1636:1662	The challenge assay results	1636:1662	The challenge assay results revealed that the mortality rate of HS diet was higher than those of LS and MS diets, but the difference was not significant (P > 0.05).
33758306	5	66	theme	high	765:768	arg1	diet					781:784	The high cornstarch diet	761:784	The high cornstarch diet	761:784	The high cornstarch diet significantly decreased the content of crude protein and increased the content of crude lipid in whole body composition (P < 0.05).
33758306	8	67	theme	HS	1309:1310	arg1	P < 0.05					1318:1325	P < 0.05	1318:1325	P < 0.05	1318:1325	The results of antioxidant experiments demonstrated that the content of SOD and T-AOC in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	8	67	theme	HS	1309:1310	arg1	diet					1312:1315	HS diet	1309:1315	HS diet (P < 0.05)	1309:1326	The results of antioxidant experiments demonstrated that the content of SOD and T-AOC in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	2	68	dep	fed	279:281	arg1	low-cornstarch					313:326	low-cornstarch	313:326	low-cornstarch	313:326	All test fish were fed with three cornstarch levels: low-cornstarch (0, LS), medium-cornstarch (18%, MS) and high-cornstarch (36%, HS) diets.
33758306	2	68	dep	fed	279:281	arg1	medium-cornstarch					337:353	medium-cornstarch	337:353	medium-cornstarch	337:353	All test fish were fed with three cornstarch levels: low-cornstarch (0, LS), medium-cornstarch (18%, MS) and high-cornstarch (36%, HS) diets.
33758306	2	68	dep	fed	279:281	arg1	high-cornstarch					369:383	high-cornstarch	369:383	high-cornstarch	369:383	All test fish were fed with three cornstarch levels: low-cornstarch (0, LS), medium-cornstarch (18%, MS) and high-cornstarch (36%, HS) diets.
33758306	1	69	from	levels	216:221	arg1	diet					234:237	tilapia diet	226:237	tilapia diet	226:237	This study was conducted to investigate the relationship between different cornstarch levels in tilapia diet and immune function.
33758306	1	69	from	levels	216:221	arg1	function					250:257	immune function	243:257	immune function	243:257	This study was conducted to investigate the relationship between different cornstarch levels in tilapia diet and immune function.
33758306	7	70	theme	biochemical	1035:1045	arg1	index					1047:1051	blood biochemical index	1029:1051	blood biochemical index	1029:1051	The results of blood biochemical index exhibited that the HS diet significantly increased the content of blood glucose, and liver/muscle glycogen (P < 0.05).
33758306	9	71	from	content	1344:1350	arg1	diet					1365:1368	MS diet	1362:1368	MS diet	1362:1368	Meanwhile, the content of MDA in MS diet was significantly lower than that of HS diet (P < 0.05).
33758306	4	72	theme	cornstarch	592:601	arg1	diets					603:607	the low and medium cornstarch diets	573:607	diets	603:607	Compared with the low and medium cornstarch diets, the results of growth showed that the high cornstarch diet significantly decreased the FBW, WGR, and SGR, and increased the FCR of tilapia (P < 0.05).
33758306	0	73	theme	immune	77:82	arg1	status					84:89	immune status	77:89	immune status	77:89	Impact of high dietary cornstarch level on growth, antioxidant response, and immune status in GIFT tilapia Oreochromis niloticus.
33758306	5	74	theme	crude	825:829	arg1	protein					831:837	crude protein	825:837	crude protein	825:837	The high cornstarch diet significantly decreased the content of crude protein and increased the content of crude lipid in whole body composition (P < 0.05).
33758306	0	75	from	growth	43:48	arg1	niloticus					119:127	GIFT tilapia Oreochromis niloticus	94:127	GIFT tilapia Oreochromis niloticus	94:127	Impact of high dietary cornstarch level on growth, antioxidant response, and immune status in GIFT tilapia Oreochromis niloticus.
33758306	4	76	theme	high	648:651	arg1	diet					664:667	the high cornstarch diet	644:667	the high cornstarch diet	644:667	Compared with the low and medium cornstarch diets, the results of growth showed that the high cornstarch diet significantly decreased the FBW, WGR, and SGR, and increased the FCR of tilapia (P < 0.05).
33758306	2	77	dep	high-cornstarch	369:383	arg1	HS					391:392	HS	391:392	HS	391:392	All test fish were fed with three cornstarch levels: low-cornstarch (0, LS), medium-cornstarch (18%, MS) and high-cornstarch (36%, HS) diets.
33758306	2	77	dep	high-cornstarch	369:383	arg1	%					388:388	36%	386:388	36%	386:388	All test fish were fed with three cornstarch levels: low-cornstarch (0, LS), medium-cornstarch (18%, MS) and high-cornstarch (36%, HS) diets.
33758306	7	78	theme	liver/muscle	1138:1149	arg1	P < 0.05					1161:1168	P < 0.05	1161:1168	P < 0.05	1161:1168	The results of blood biochemical index exhibited that the HS diet significantly increased the content of blood glucose, and liver/muscle glycogen (P < 0.05).
33758306	7	78	theme	liver/muscle	1138:1149	arg1	glycogen					1151:1158	liver/muscle glycogen	1138:1158	liver/muscle glycogen (P < 0.05)	1138:1169	The results of blood biochemical index exhibited that the HS diet significantly increased the content of blood glucose, and liver/muscle glycogen (P < 0.05).
33758306	3	79	theme	initial	432:438	arg1	weight					450:455	initial mean body weight 31.73 ± 1.36 g	432:470	initial mean body weight 31.73 ± 1.36 g	432:470	Three hundred and sixty fish (initial mean body weight 31.73 ± 1.36 g) were randomly allocated into twelve water-circulated tanks, and thirty fish per tank.
33758306	3	79	theme	initial	432:438	arg1	fish					426:429	Three hundred and sixty fish	402:429	Three hundred and sixty fish (initial mean body weight 31.73 ± 1.36 g)	402:471	Three hundred and sixty fish (initial mean body weight 31.73 ± 1.36 g) were randomly allocated into twelve water-circulated tanks, and thirty fish per tank.
33758306	4	80	theme	growth	625:630	arg1	results					614:620	the results	610:620	the results of growth	610:630	Compared with the low and medium cornstarch diets, the results of growth showed that the high cornstarch diet significantly decreased the FBW, WGR, and SGR, and increased the FCR of tilapia (P < 0.05).
33758306	0	81	from	response	63:70	arg1	niloticus					119:127	GIFT tilapia Oreochromis niloticus	94:127	GIFT tilapia Oreochromis niloticus	94:127	Impact of high dietary cornstarch level on growth, antioxidant response, and immune status in GIFT tilapia Oreochromis niloticus.
33758306	0	82	theme	dietary	15:21	arg1	level					34:38	high dietary cornstarch level	10:38	high dietary cornstarch level	10:38	Impact of high dietary cornstarch level on growth, antioxidant response, and immune status in GIFT tilapia Oreochromis niloticus.
33758306	10	83	from	activity	1546:1553	arg1	serum					1503:1507	serum	1503:1507	serum	1503:1507	The results of immune index test showed that the lysozyme activities in the serum, liver, and gill, and the phagocytic activity and index in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	10	83	from	activity	1546:1553	arg1	diet					1571:1574	MS diet	1568:1574	MS diet	1568:1574	The results of immune index test showed that the lysozyme activities in the serum, liver, and gill, and the phagocytic activity and index in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	10	83	from	activity	1546:1553	arg1	liver					1510:1514	liver	1510:1514	liver	1510:1514	The results of immune index test showed that the lysozyme activities in the serum, liver, and gill, and the phagocytic activity and index in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	10	83	from	activity	1546:1553	arg1	gill					1521:1524	gill	1521:1524	gill	1521:1524	The results of immune index test showed that the lysozyme activities in the serum, liver, and gill, and the phagocytic activity and index in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	7	84	theme	blood	1119:1123	arg1	glucose					1125:1131	blood glucose	1119:1131	blood glucose	1119:1131	The results of blood biochemical index exhibited that the HS diet significantly increased the content of blood glucose, and liver/muscle glycogen (P < 0.05).
33758306	2	85	dep	low-cornstarch	313:326	arg1	diets					395:399	diets	395:399	diets	395:399	All test fish were fed with three cornstarch levels: low-cornstarch (0, LS), medium-cornstarch (18%, MS) and high-cornstarch (36%, HS) diets.
33758306	2	85	dep	low-cornstarch	313:326	arg1	LS					332:333	LS	332:333	LS	332:333	All test fish were fed with three cornstarch levels: low-cornstarch (0, LS), medium-cornstarch (18%, MS) and high-cornstarch (36%, HS) diets.
33758306	2	85	dep	low-cornstarch	313:326	arg1	0					329:329	0	329:329	0	329:329	All test fish were fed with three cornstarch levels: low-cornstarch (0, LS), medium-cornstarch (18%, MS) and high-cornstarch (36%, HS) diets.
33758306	11	86	theme	challenge	1640:1648	arg1	results					1656:1662	The challenge assay results	1636:1662	The challenge assay results	1636:1662	The challenge assay results revealed that the mortality rate of HS diet was higher than those of LS and MS diets, but the difference was not significant (P > 0.05).
33758306	0	87	theme	level	34:38	arg1	Impact					0:5	Impact	0:5	Impact of high dietary cornstarch level on growth, antioxidant response, and immune status in GIFT tilapia Oreochromis niloticus.	0:128	Impact of high dietary cornstarch level on growth, antioxidant response, and immune status in GIFT tilapia Oreochromis niloticus.
33758306	5	88	theme	lipid	874:878	arg1	content					857:863	the content	853:863	the content of crude lipid in whole body composition (P < 0.05)	853:915	The high cornstarch diet significantly decreased the content of crude protein and increased the content of crude lipid in whole body composition (P < 0.05).
33758306	10	89	from	index	1559:1563	arg1	serum					1503:1507	serum	1503:1507	serum	1503:1507	The results of immune index test showed that the lysozyme activities in the serum, liver, and gill, and the phagocytic activity and index in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	10	89	from	index	1559:1563	arg1	diet					1571:1574	MS diet	1568:1574	MS diet	1568:1574	The results of immune index test showed that the lysozyme activities in the serum, liver, and gill, and the phagocytic activity and index in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	10	89	from	index	1559:1563	arg1	liver					1510:1514	liver	1510:1514	liver	1510:1514	The results of immune index test showed that the lysozyme activities in the serum, liver, and gill, and the phagocytic activity and index in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	10	89	from	index	1559:1563	arg1	gill					1521:1524	gill	1521:1524	gill	1521:1524	The results of immune index test showed that the lysozyme activities in the serum, liver, and gill, and the phagocytic activity and index in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	8	90	theme	experiments	1199:1209	arg1	results					1176:1182	The results	1172:1182	The results of antioxidant experiments	1172:1209	The results of antioxidant experiments demonstrated that the content of SOD and T-AOC in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	5	91	theme	whole	883:887	arg1	P < 0.05					907:914	P < 0.05	907:914	P < 0.05	907:914	The high cornstarch diet significantly decreased the content of crude protein and increased the content of crude lipid in whole body composition (P < 0.05).
33758306	5	91	theme	whole	883:887	arg1	composition					894:904	whole body composition	883:904	whole body composition (P < 0.05)	883:915	The high cornstarch diet significantly decreased the content of crude protein and increased the content of crude lipid in whole body composition (P < 0.05).
33758306	10	92	theme	test	1455:1458	arg1	results					1431:1437	The results	1427:1437	The results of immune index test	1427:1458	The results of immune index test showed that the lysozyme activities in the serum, liver, and gill, and the phagocytic activity and index in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	9	93	theme	HS	1407:1408	arg1	P < 0.05					1416:1423	P < 0.05	1416:1423	P < 0.05	1416:1423	Meanwhile, the content of MDA in MS diet was significantly lower than that of HS diet (P < 0.05).
33758306	9	93	theme	HS	1407:1408	arg1	diet					1410:1413	HS diet	1407:1413	HS diet (P < 0.05)	1407:1424	Meanwhile, the content of MDA in MS diet was significantly lower than that of HS diet (P < 0.05).
33758306	13	94	theme	18	2003:2004	arg1	%					2005:2005	%	2005:2005	%	2005:2005	Under this experimental condition, GIFT tilapia could tolerate 18% cornstarch, but not 36% cornstarch.
33758306	10	95	theme	immune	1442:1447	arg1	test					1455:1458	immune index test	1442:1458	immune index test	1442:1458	The results of immune index test showed that the lysozyme activities in the serum, liver, and gill, and the phagocytic activity and index in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	8	96	theme	SOD	1244:1246	arg1	higher					1288:1293	higher	1288:1293	higher	1288:1293	The results of antioxidant experiments demonstrated that the content of SOD and T-AOC in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	8	96	theme	SOD	1244:1246	arg1	content					1233:1239	the content	1229:1239	the content of SOD and T-AOC in MS diet	1229:1267	The results of antioxidant experiments demonstrated that the content of SOD and T-AOC in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	10	97	theme	lysozyme	1476:1483	arg1	higher					1595:1600	higher	1595:1600	higher	1595:1600	The results of immune index test showed that the lysozyme activities in the serum, liver, and gill, and the phagocytic activity and index in MS diet were significantly higher than those of HS diet (P < 0.05).
33758306	10	97	theme	lysozyme	1476:1483	arg1	activities					1485:1494	the lysozyme activities	1472:1494	the lysozyme activities in the serum, liver, and gill	1472:1524	The results of immune index test showed that the lysozyme activities in the serum, liver, and gill, and the phagocytic activity and index in MS diet were significantly higher than those of HS diet (P < 0.05).
32823755	5	0	from	residues	965:972	arg1	FOS					933:935	FOS	933:935	FOS	933:935	We investigated the effect of milk supplementation with a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive"), exerted on preweaned lamb growth and the composition of their fecal microbiota, by using 16S rRNA gene amplicon high-throughput sequencing.
32823755	5	0	from	residues	965:972	arg1	fructooligosaccharides					909:930	fructooligosaccharides	909:930	fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive")	909:1007	We investigated the effect of milk supplementation with a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive"), exerted on preweaned lamb growth and the composition of their fecal microbiota, by using 16S rRNA gene amplicon high-throughput sequencing.
32823755	5	0	from	residues	965:972	arg1	prebiotics					897:906	two well-known prebiotics	882:906	two well-known prebiotics	882:906	We investigated the effect of milk supplementation with a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive"), exerted on preweaned lamb growth and the composition of their fecal microbiota, by using 16S rRNA gene amplicon high-throughput sequencing.
32823755	1	1	theme	newborn	259:265	arg1	ruminants					267:275	newborn ruminants	259:275	newborn ruminants	259:275	The proper development of the early gastrointestinal tract (GIT) microbiota is critical for newborn ruminants.
32823755	9	2	from	beet	1794:1797	arg1	FOS					1779:1781	FOS	1779:1781	FOS from sugar beet and garlic residues as a potential prebiotic to be used in young ruminants' nutrition in order to improve production yields	1779:1921	This study points out the combination of FOS from sugar beet and garlic residues as a potential prebiotic to be used in young ruminants' nutrition in order to improve production yields.
32823755	9	2	from	beet	1794:1797	arg1	combination					1764:1774	the combination	1760:1774	the combination of FOS from sugar beet and garlic residues as a potential prebiotic to be used in young ruminants' nutrition in order to improve production yields	1760:1921	This study points out the combination of FOS from sugar beet and garlic residues as a potential prebiotic to be used in young ruminants' nutrition in order to improve production yields.
32823755	6	3	theme	mean	1199:1202	arg1	gain					1217:1220	the mean daily weight gain	1195:1220	the mean daily weight gain of lambs	1195:1229	The results showed a significant increase in the mean daily weight gain of lambs fed with the additive.
32823755	8	4	theme	additive	1682:1689	arg1	intake					1691:1696	the additive intake	1678:1696	the additive intake	1678:1696	These bacteria have been previously reported to confer beneficial properties to the ruminant, including promotion of growth and health status, and our results showed that they were strongly linked to the additive intake and the increased weight gain of lambs.
32823755	9	5	theme	young	1858:1862	arg1	ruminants					1864:1872	young ruminants'	1858:1873	young ruminants' nutrition	1858:1883	This study points out the combination of FOS from sugar beet and garlic residues as a potential prebiotic to be used in young ruminants' nutrition in order to improve production yields.
32823755	6	6	theme	lambs	1225:1229	arg1	gain					1217:1220	the mean daily weight gain	1195:1220	the mean daily weight gain of lambs	1195:1229	The results showed a significant increase in the mean daily weight gain of lambs fed with the additive.
32823755	7	7	from	Bifidobacterium	1416:1430	arg1	abundant					1404:1411	abundant	1404:1411	abundant	1404:1411	Lamb fecal microbiota was also influenced by the additive intake, as additive-diet lambs showed lower bacterial diversity and were significantly more abundant in Bifidobacterium, Enterococcus, Lactobacillus and Veillonella.
32823755	8	8	theme	increased	1706:1714	arg1	gain					1723:1726	the increased weight gain	1702:1726	the increased weight gain of lambs	1702:1735	These bacteria have been previously reported to confer beneficial properties to the ruminant, including promotion of growth and health status, and our results showed that they were strongly linked to the additive intake and the increased weight gain of lambs.
32823755	1	9	theme	early	197:201	arg1	microbiota					232:241	the early gastrointestinal tract (GIT) microbiota	193:241	the early gastrointestinal tract (GIT) microbiota	193:241	The proper development of the early gastrointestinal tract (GIT) microbiota is critical for newborn ruminants.
32823755	7	10	theme	bacterial	1356:1364	arg1	diversity					1366:1374	lower bacterial diversity	1350:1374	lower bacterial diversity	1350:1374	Lamb fecal microbiota was also influenced by the additive intake, as additive-diet lambs showed lower bacterial diversity and were significantly more abundant in Bifidobacterium, Enterococcus, Lactobacillus and Veillonella.
32823755	0	11	theme	Weight	136:141	arg1	Gain					143:146	Weight Gain	136:146	Weight Gain in Neonatal Lambs	136:164	Dietary Supplementation with Sugar Beet Fructooligosaccharides and Garlic Residues Promotes Growth of Beneficial Bacteria and Increases Weight Gain in Neonatal Lambs.
32823755	3	12	theme	GIT	578:580	arg1	bacteria					582:589	beneficial GIT bacteria	567:589	beneficial GIT bacteria	567:589	Dietary supplementation with prebiotics, ingredients nondigestible and nonabsorbable by the host that stimulate the growth of beneficial GIT bacteria, has been applied worldwide as a potential approach in order to improve ruminant health and production yields.
32823755	5	13	theme	milk	839:842	arg1	supplementation					844:858	milk supplementation	839:858	milk supplementation	839:858	We investigated the effect of milk supplementation with a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive"), exerted on preweaned lamb growth and the composition of their fecal microbiota, by using 16S rRNA gene amplicon high-throughput sequencing.
32823755	6	14	theme	weight	1210:1215	arg1	gain					1217:1220	the mean daily weight gain	1195:1220	the mean daily weight gain of lambs	1195:1229	The results showed a significant increase in the mean daily weight gain of lambs fed with the additive.
32823755	6	15	dep	showed	1162:1167	arg1	fed					1231:1233	fed	1231:1233	showed a significant increase in the mean daily weight gain of lambs fed with the additive	1162:1251	The results showed a significant increase in the mean daily weight gain of lambs fed with the additive.
32823755	8	16	theme	lambs	1731:1735	arg1	intake					1691:1696	the additive intake	1678:1696	the additive intake	1678:1696	These bacteria have been previously reported to confer beneficial properties to the ruminant, including promotion of growth and health status, and our results showed that they were strongly linked to the additive intake and the increased weight gain of lambs.
32823755	8	16	theme	lambs	1731:1735	arg1	gain					1723:1726	the increased weight gain	1702:1726	the increased weight gain of lambs	1702:1735	These bacteria have been previously reported to confer beneficial properties to the ruminant, including promotion of growth and health status, and our results showed that they were strongly linked to the additive intake and the increased weight gain of lambs.
32823755	1	17	theme	GIT	227:229	arg1	microbiota					232:241	the early gastrointestinal tract (GIT) microbiota	193:241	the early gastrointestinal tract (GIT) microbiota	193:241	The proper development of the early gastrointestinal tract (GIT) microbiota is critical for newborn ruminants.
32823755	7	18	theme	additive-diet	1323:1335	arg1	lambs					1337:1341	additive-diet lambs	1323:1341	additive-diet lambs	1323:1341	Lamb fecal microbiota was also influenced by the additive intake, as additive-diet lambs showed lower bacterial diversity and were significantly more abundant in Bifidobacterium, Enterococcus, Lactobacillus and Veillonella.
32823755	9	19	theme	production	1905:1914	arg1	yields					1916:1921	production yields	1905:1921	production yields	1905:1921	This study points out the combination of FOS from sugar beet and garlic residues as a potential prebiotic to be used in young ruminants' nutrition in order to improve production yields.
32823755	5	20	dep	residues	965:972	arg1	named					988:992	named	988:992	together named as "additive"	979:1006	We investigated the effect of milk supplementation with a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive"), exerted on preweaned lamb growth and the composition of their fecal microbiota, by using 16S rRNA gene amplicon high-throughput sequencing.
32823755	5	21	theme	lamb	1031:1034	arg1	growth					1036:1041	preweaned lamb growth	1021:1041	preweaned lamb growth	1021:1041	We investigated the effect of milk supplementation with a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive"), exerted on preweaned lamb growth and the composition of their fecal microbiota, by using 16S rRNA gene amplicon high-throughput sequencing.
32823755	7	22	theme	additive	1303:1310	arg1	intake					1312:1317	the additive intake	1299:1317	the additive intake	1299:1317	Lamb fecal microbiota was also influenced by the additive intake, as additive-diet lambs showed lower bacterial diversity and were significantly more abundant in Bifidobacterium, Enterococcus, Lactobacillus and Veillonella.
32823755	5	23	theme	prebiotics	897:906	arg1	combination					867:877	a combination	865:877	a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive")	865:1007	We investigated the effect of milk supplementation with a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive"), exerted on preweaned lamb growth and the composition of their fecal microbiota, by using 16S rRNA gene amplicon high-throughput sequencing.
32823755	5	24	theme	16S	1099:1101	arg1	sequencing					1138:1147	16S rRNA gene amplicon high-throughput sequencing	1099:1147	16S rRNA gene amplicon high-throughput sequencing	1099:1147	We investigated the effect of milk supplementation with a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive"), exerted on preweaned lamb growth and the composition of their fecal microbiota, by using 16S rRNA gene amplicon high-throughput sequencing.
32823755	1	25	theme	proper	171:176	arg1	development					178:188	The proper development	167:188	The proper development of the early gastrointestinal tract (GIT) microbiota	167:241	The proper development of the early gastrointestinal tract (GIT) microbiota is critical for newborn ruminants.
32823755	1	25	theme	proper	171:176	arg1	critical					246:253	critical	246:253	critical	246:253	The proper development of the early gastrointestinal tract (GIT) microbiota is critical for newborn ruminants.
32823755	7	26	from	abundant	1404:1411	arg1	Enterococcus					1433:1444	Enterococcus	1433:1444	Enterococcus	1433:1444	Lamb fecal microbiota was also influenced by the additive intake, as additive-diet lambs showed lower bacterial diversity and were significantly more abundant in Bifidobacterium, Enterococcus, Lactobacillus and Veillonella.
32823755	7	26	from	abundant	1404:1411	arg1	Bifidobacterium					1416:1430	Bifidobacterium	1416:1430	Bifidobacterium	1416:1430	Lamb fecal microbiota was also influenced by the additive intake, as additive-diet lambs showed lower bacterial diversity and were significantly more abundant in Bifidobacterium, Enterococcus, Lactobacillus and Veillonella.
32823755	7	26	from	abundant	1404:1411	arg1	Veillonella					1465:1475	Veillonella	1465:1475	Veillonella	1465:1475	Lamb fecal microbiota was also influenced by the additive intake, as additive-diet lambs showed lower bacterial diversity and were significantly more abundant in Bifidobacterium, Enterococcus, Lactobacillus and Veillonella.
32823755	7	26	from	abundant	1404:1411	arg1	Lactobacillus					1447:1459	Lactobacillus	1447:1459	Lactobacillus	1447:1459	Lamb fecal microbiota was also influenced by the additive intake, as additive-diet lambs showed lower bacterial diversity and were significantly more abundant in Bifidobacterium, Enterococcus, Lactobacillus and Veillonella.
32823755	4	27	theme	early	770:774	arg1	life					776:779	ruminants' early life	759:779	ruminants' early life	759:779	However, how prebiotics affect the GIT microbiota during ruminants' early life is still poorly understood.
32823755	6	28	from	increase	1183:1190	arg1	gain					1217:1220	the mean daily weight gain	1195:1220	the mean daily weight gain of lambs	1195:1229	The results showed a significant increase in the mean daily weight gain of lambs fed with the additive.
32823755	0	29	theme	Beneficial	102:111	arg1	Bacteria					113:120	Beneficial Bacteria	102:120	Beneficial Bacteria	102:120	Dietary Supplementation with Sugar Beet Fructooligosaccharides and Garlic Residues Promotes Growth of Beneficial Bacteria and Increases Weight Gain in Neonatal Lambs.
32823755	2	30	theme	long-lasting	385:396	arg1	results					398:404	long-lasting results	385:404	long-lasting results	385:404	This microbiota is susceptible to modification by diverse external factors (such as diet) that can lead to long-lasting results when occurring in young ruminants.
32823755	5	31	theme	microbiota	1078:1087	arg1	growth					1036:1041	preweaned lamb growth	1021:1041	preweaned lamb growth	1021:1041	We investigated the effect of milk supplementation with a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive"), exerted on preweaned lamb growth and the composition of their fecal microbiota, by using 16S rRNA gene amplicon high-throughput sequencing.
32823755	5	31	theme	microbiota	1078:1087	arg1	composition					1051:1061	the composition	1047:1061	the composition of their fecal microbiota	1047:1087	We investigated the effect of milk supplementation with a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive"), exerted on preweaned lamb growth and the composition of their fecal microbiota, by using 16S rRNA gene amplicon high-throughput sequencing.
32823755	2	32	dep	factors	345:351	arg1	such					354:357	such	354:357	such	354:357	This microbiota is susceptible to modification by diverse external factors (such as diet) that can lead to long-lasting results when occurring in young ruminants.
32823755	0	33	theme	Dietary	0:6	arg1	Supplementation					8:22	Dietary Supplementation	0:22	Dietary Supplementation with Sugar Beet Fructooligosaccharides and Garlic Residues	0:81	Dietary Supplementation with Sugar Beet Fructooligosaccharides and Garlic Residues Promotes Growth of Beneficial Bacteria and Increases Weight Gain in Neonatal Lambs.
32823755	9	34	theme	FOS	1779:1781	arg1	combination					1764:1774	the combination	1760:1774	the combination of FOS from sugar beet and garlic residues as a potential prebiotic to be used in young ruminants' nutrition in order to improve production yields	1760:1921	This study points out the combination of FOS from sugar beet and garlic residues as a potential prebiotic to be used in young ruminants' nutrition in order to improve production yields.
32823755	8	35	theme	beneficial	1533:1542	arg1	properties					1544:1553	beneficial properties	1533:1553	beneficial properties	1533:1553	These bacteria have been previously reported to confer beneficial properties to the ruminant, including promotion of growth and health status, and our results showed that they were strongly linked to the additive intake and the increased weight gain of lambs.
32823755	5	36	with	supplementation	844:858	arg1	combination					867:877	a combination	865:877	a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive")	865:1007	We investigated the effect of milk supplementation with a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive"), exerted on preweaned lamb growth and the composition of their fecal microbiota, by using 16S rRNA gene amplicon high-throughput sequencing.
32823755	5	37	theme	garlic	958:963	arg1	residues					965:972	garlic residues	958:972	garlic residues (all together named as "additive")	958:1007	We investigated the effect of milk supplementation with a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive"), exerted on preweaned lamb growth and the composition of their fecal microbiota, by using 16S rRNA gene amplicon high-throughput sequencing.
32823755	9	38	theme	sugar	1788:1792	arg1	beet					1794:1797	sugar beet	1788:1797	sugar beet	1788:1797	This study points out the combination of FOS from sugar beet and garlic residues as a potential prebiotic to be used in young ruminants' nutrition in order to improve production yields.
32823755	0	39	theme	Beet	35:38	arg1	Fructooligosaccharides					40:61	Sugar Beet Fructooligosaccharides	29:61	Sugar Beet Fructooligosaccharides	29:61	Dietary Supplementation with Sugar Beet Fructooligosaccharides and Garlic Residues Promotes Growth of Beneficial Bacteria and Increases Weight Gain in Neonatal Lambs.
32823755	5	40	theme	gene	1108:1111	arg1	sequencing					1138:1147	16S rRNA gene amplicon high-throughput sequencing	1099:1147	16S rRNA gene amplicon high-throughput sequencing	1099:1147	We investigated the effect of milk supplementation with a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive"), exerted on preweaned lamb growth and the composition of their fecal microbiota, by using 16S rRNA gene amplicon high-throughput sequencing.
32823755	7	41	theme	fecal	1259:1263	arg1	microbiota					1265:1274	Lamb fecal microbiota	1254:1274	Lamb fecal microbiota	1254:1274	Lamb fecal microbiota was also influenced by the additive intake, as additive-diet lambs showed lower bacterial diversity and were significantly more abundant in Bifidobacterium, Enterococcus, Lactobacillus and Veillonella.
32823755	8	42	theme	ruminant	1562:1569	arg1	promotion					1582:1590	the ruminant, including promotion	1558:1590	the ruminant, including promotion of growth and health status	1558:1618	These bacteria have been previously reported to confer beneficial properties to the ruminant, including promotion of growth and health status, and our results showed that they were strongly linked to the additive intake and the increased weight gain of lambs.
32823755	5	43	theme	high-throughput	1122:1136	arg1	sequencing					1138:1147	16S rRNA gene amplicon high-throughput sequencing	1099:1147	16S rRNA gene amplicon high-throughput sequencing	1099:1147	We investigated the effect of milk supplementation with a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive"), exerted on preweaned lamb growth and the composition of their fecal microbiota, by using 16S rRNA gene amplicon high-throughput sequencing.
32823755	3	44	theme	production	683:692	arg1	yields					694:699	ruminant health and production yields	663:699	yields	694:699	Dietary supplementation with prebiotics, ingredients nondigestible and nonabsorbable by the host that stimulate the growth of beneficial GIT bacteria, has been applied worldwide as a potential approach in order to improve ruminant health and production yields.
32823755	5	45	from	beet	949:952	arg1	FOS					933:935	FOS	933:935	FOS	933:935	We investigated the effect of milk supplementation with a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive"), exerted on preweaned lamb growth and the composition of their fecal microbiota, by using 16S rRNA gene amplicon high-throughput sequencing.
32823755	5	45	from	beet	949:952	arg1	fructooligosaccharides					909:930	fructooligosaccharides	909:930	fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive")	909:1007	We investigated the effect of milk supplementation with a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive"), exerted on preweaned lamb growth and the composition of their fecal microbiota, by using 16S rRNA gene amplicon high-throughput sequencing.
32823755	5	45	from	beet	949:952	arg1	prebiotics					897:906	two well-known prebiotics	882:906	two well-known prebiotics	882:906	We investigated the effect of milk supplementation with a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive"), exerted on preweaned lamb growth and the composition of their fecal microbiota, by using 16S rRNA gene amplicon high-throughput sequencing.
32823755	5	46	theme	additive	998:1005	arg1	"					1006:1006	"additive"	997:1006	"additive"	997:1006	We investigated the effect of milk supplementation with a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive"), exerted on preweaned lamb growth and the composition of their fecal microbiota, by using 16S rRNA gene amplicon high-throughput sequencing.
32823755	8	47	theme	status	1613:1618	arg1	promotion					1582:1590	the ruminant, including promotion	1558:1590	the ruminant, including promotion of growth and health status	1558:1618	These bacteria have been previously reported to confer beneficial properties to the ruminant, including promotion of growth and health status, and our results showed that they were strongly linked to the additive intake and the increased weight gain of lambs.
32823755	3	48	theme	bacteria	582:589	arg1	growth					557:562	the growth	553:562	the growth of beneficial GIT bacteria	553:589	Dietary supplementation with prebiotics, ingredients nondigestible and nonabsorbable by the host that stimulate the growth of beneficial GIT bacteria, has been applied worldwide as a potential approach in order to improve ruminant health and production yields.
32823755	9	49	from	combination	1764:1774	arg1	beet					1794:1797	sugar beet	1788:1797	sugar beet	1788:1797	This study points out the combination of FOS from sugar beet and garlic residues as a potential prebiotic to be used in young ruminants' nutrition in order to improve production yields.
32823755	9	49	from	combination	1764:1774	arg1	residues					1810:1817	garlic residues	1803:1817	garlic residues	1803:1817	This study points out the combination of FOS from sugar beet and garlic residues as a potential prebiotic to be used in young ruminants' nutrition in order to improve production yields.
32823755	2	50	theme	diverse	328:334	arg1	factors					345:351	diverse external factors	328:351	diverse external factors (such as diet) that can lead to long-lasting results when occurring in young ruminants	328:438	This microbiota is susceptible to modification by diverse external factors (such as diet) that can lead to long-lasting results when occurring in young ruminants.
32823755	6	51	theme	significant	1171:1181	arg1	increase					1183:1190	a significant increase	1169:1190	a significant increase in the mean daily weight gain of lambs	1169:1229	The results showed a significant increase in the mean daily weight gain of lambs fed with the additive.
32823755	3	52	with	supplementation	449:463	arg1	ingredients					482:492	ingredients	482:492	ingredients nondigestible and nonabsorbable by the host that stimulate the growth of beneficial GIT bacteria	482:589	Dietary supplementation with prebiotics, ingredients nondigestible and nonabsorbable by the host that stimulate the growth of beneficial GIT bacteria, has been applied worldwide as a potential approach in order to improve ruminant health and production yields.
32823755	3	52	with	supplementation	449:463	arg1	prebiotics					470:479	prebiotics	470:479	prebiotics	470:479	Dietary supplementation with prebiotics, ingredients nondigestible and nonabsorbable by the host that stimulate the growth of beneficial GIT bacteria, has been applied worldwide as a potential approach in order to improve ruminant health and production yields.
32823755	3	53	theme	beneficial	567:576	arg1	bacteria					582:589	beneficial GIT bacteria	567:589	beneficial GIT bacteria	567:589	Dietary supplementation with prebiotics, ingredients nondigestible and nonabsorbable by the host that stimulate the growth of beneficial GIT bacteria, has been applied worldwide as a potential approach in order to improve ruminant health and production yields.
32823755	1	54	theme	gastrointestinal	203:218	arg1	microbiota					232:241	the early gastrointestinal tract (GIT) microbiota	193:241	the early gastrointestinal tract (GIT) microbiota	193:241	The proper development of the early gastrointestinal tract (GIT) microbiota is critical for newborn ruminants.
32823755	0	55	theme	Neonatal	151:158	arg1	Lambs					160:164	Neonatal Lambs	151:164	Neonatal Lambs	151:164	Dietary Supplementation with Sugar Beet Fructooligosaccharides and Garlic Residues Promotes Growth of Beneficial Bacteria and Increases Weight Gain in Neonatal Lambs.
32823755	8	56	theme	weight	1716:1721	arg1	gain					1723:1726	the increased weight gain	1702:1726	the increased weight gain of lambs	1702:1735	These bacteria have been previously reported to confer beneficial properties to the ruminant, including promotion of growth and health status, and our results showed that they were strongly linked to the additive intake and the increased weight gain of lambs.
32823755	5	57	theme	supplementation	844:858	arg1	effect					829:834	the effect	825:834	the effect of milk supplementation with a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive"), exerted on preweaned lamb growth and the composition of their fecal microbiota,	825:1088	We investigated the effect of milk supplementation with a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive"), exerted on preweaned lamb growth and the composition of their fecal microbiota, by using 16S rRNA gene amplicon high-throughput sequencing.
32823755	6	58	theme	daily	1204:1208	arg1	gain					1217:1220	the mean daily weight gain	1195:1220	the mean daily weight gain of lambs	1195:1229	The results showed a significant increase in the mean daily weight gain of lambs fed with the additive.
32823755	8	59	dep	ruminant	1562:1569	arg1	including					1572:1580	including	1572:1580	including	1572:1580	These bacteria have been previously reported to confer beneficial properties to the ruminant, including promotion of growth and health status, and our results showed that they were strongly linked to the additive intake and the increased weight gain of lambs.
32823755	5	60	theme	preweaned	1021:1029	arg1	growth					1036:1041	preweaned lamb growth	1021:1041	preweaned lamb growth	1021:1041	We investigated the effect of milk supplementation with a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive"), exerted on preweaned lamb growth and the composition of their fecal microbiota, by using 16S rRNA gene amplicon high-throughput sequencing.
32823755	3	61	theme	potential	624:632	arg1	worldwide					609:617	worldwide	609:617	worldwide	609:617	Dietary supplementation with prebiotics, ingredients nondigestible and nonabsorbable by the host that stimulate the growth of beneficial GIT bacteria, has been applied worldwide as a potential approach in order to improve ruminant health and production yields.
32823755	3	61	theme	potential	624:632	arg1	supplementation					449:463	Dietary supplementation	441:463	Dietary supplementation with prebiotics, ingredients nondigestible and nonabsorbable by the host that stimulate the growth of beneficial GIT bacteria,	441:590	Dietary supplementation with prebiotics, ingredients nondigestible and nonabsorbable by the host that stimulate the growth of beneficial GIT bacteria, has been applied worldwide as a potential approach in order to improve ruminant health and production yields.
32823755	3	61	theme	potential	624:632	arg1	approach					634:641	a potential approach	622:641	a potential approach in order to improve ruminant health and production yields	622:699	Dietary supplementation with prebiotics, ingredients nondigestible and nonabsorbable by the host that stimulate the growth of beneficial GIT bacteria, has been applied worldwide as a potential approach in order to improve ruminant health and production yields.
32823755	9	62	from	residues	1810:1817	arg1	FOS					1779:1781	FOS	1779:1781	FOS from sugar beet and garlic residues as a potential prebiotic to be used in young ruminants' nutrition in order to improve production yields	1779:1921	This study points out the combination of FOS from sugar beet and garlic residues as a potential prebiotic to be used in young ruminants' nutrition in order to improve production yields.
32823755	9	62	from	residues	1810:1817	arg1	combination					1764:1774	the combination	1760:1774	the combination of FOS from sugar beet and garlic residues as a potential prebiotic to be used in young ruminants' nutrition in order to improve production yields	1760:1921	This study points out the combination of FOS from sugar beet and garlic residues as a potential prebiotic to be used in young ruminants' nutrition in order to improve production yields.
32823755	8	63	attach	linked	1668:1673	arg1	intake					1691:1696	the additive intake	1678:1696	the additive intake	1678:1696	These bacteria have been previously reported to confer beneficial properties to the ruminant, including promotion of growth and health status, and our results showed that they were strongly linked to the additive intake and the increased weight gain of lambs.
32823755	8	63	attach	linked	1668:1673	arg2	they					1649:1652	they	1649:1652	they	1649:1652	These bacteria have been previously reported to confer beneficial properties to the ruminant, including promotion of growth and health status, and our results showed that they were strongly linked to the additive intake and the increased weight gain of lambs.
32823755	8	63	attach	linked	1668:1673	arg1	gain					1723:1726	the increased weight gain	1702:1726	the increased weight gain of lambs	1702:1735	These bacteria have been previously reported to confer beneficial properties to the ruminant, including promotion of growth and health status, and our results showed that they were strongly linked to the additive intake and the increased weight gain of lambs.
32823755	5	64	theme	well-known	886:895	arg1	fructooligosaccharides					909:930	fructooligosaccharides	909:930	fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive")	909:1007	We investigated the effect of milk supplementation with a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive"), exerted on preweaned lamb growth and the composition of their fecal microbiota, by using 16S rRNA gene amplicon high-throughput sequencing.
32823755	5	64	theme	well-known	886:895	arg1	prebiotics					897:906	two well-known prebiotics	882:906	two well-known prebiotics	882:906	We investigated the effect of milk supplementation with a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive"), exerted on preweaned lamb growth and the composition of their fecal microbiota, by using 16S rRNA gene amplicon high-throughput sequencing.
32823755	0	65	theme	Bacteria	113:120	arg1	Growth					92:97	Growth	92:97	Growth of Beneficial Bacteria	92:120	Dietary Supplementation with Sugar Beet Fructooligosaccharides and Garlic Residues Promotes Growth of Beneficial Bacteria and Increases Weight Gain in Neonatal Lambs.
32823755	5	66	theme	rRNA	1103:1106	arg1	sequencing					1138:1147	16S rRNA gene amplicon high-throughput sequencing	1099:1147	16S rRNA gene amplicon high-throughput sequencing	1099:1147	We investigated the effect of milk supplementation with a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive"), exerted on preweaned lamb growth and the composition of their fecal microbiota, by using 16S rRNA gene amplicon high-throughput sequencing.
32823755	2	67	theme	external	336:343	arg1	factors					345:351	diverse external factors	328:351	diverse external factors (such as diet) that can lead to long-lasting results when occurring in young ruminants	328:438	This microbiota is susceptible to modification by diverse external factors (such as diet) that can lead to long-lasting results when occurring in young ruminants.
32823755	5	68	theme	fecal	1072:1076	arg1	microbiota					1078:1087	their fecal microbiota	1066:1087	their fecal microbiota	1066:1087	We investigated the effect of milk supplementation with a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive"), exerted on preweaned lamb growth and the composition of their fecal microbiota, by using 16S rRNA gene amplicon high-throughput sequencing.
32823755	1	69	theme	tract	220:224	arg1	microbiota					232:241	the early gastrointestinal tract (GIT) microbiota	193:241	the early gastrointestinal tract (GIT) microbiota	193:241	The proper development of the early gastrointestinal tract (GIT) microbiota is critical for newborn ruminants.
32823755	3	70	theme	Dietary	441:447	arg1	worldwide					609:617	worldwide	609:617	worldwide	609:617	Dietary supplementation with prebiotics, ingredients nondigestible and nonabsorbable by the host that stimulate the growth of beneficial GIT bacteria, has been applied worldwide as a potential approach in order to improve ruminant health and production yields.
32823755	3	70	theme	Dietary	441:447	arg1	supplementation					449:463	Dietary supplementation	441:463	Dietary supplementation with prebiotics, ingredients nondigestible and nonabsorbable by the host that stimulate the growth of beneficial GIT bacteria,	441:590	Dietary supplementation with prebiotics, ingredients nondigestible and nonabsorbable by the host that stimulate the growth of beneficial GIT bacteria, has been applied worldwide as a potential approach in order to improve ruminant health and production yields.
32823755	3	70	theme	Dietary	441:447	arg1	approach					634:641	a potential approach	622:641	a potential approach in order to improve ruminant health and production yields	622:699	Dietary supplementation with prebiotics, ingredients nondigestible and nonabsorbable by the host that stimulate the growth of beneficial GIT bacteria, has been applied worldwide as a potential approach in order to improve ruminant health and production yields.
32823755	5	71	theme	sugar	943:947	arg1	beet					949:952	sugar beet	943:952	sugar beet	943:952	We investigated the effect of milk supplementation with a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive"), exerted on preweaned lamb growth and the composition of their fecal microbiota, by using 16S rRNA gene amplicon high-throughput sequencing.
32823755	4	72	theme	GIT	737:739	arg1	microbiota					741:750	the GIT microbiota	733:750	the GIT microbiota	733:750	However, how prebiotics affect the GIT microbiota during ruminants' early life is still poorly understood.
32823755	0	73	theme	Sugar	29:33	arg1	Fructooligosaccharides					40:61	Sugar Beet Fructooligosaccharides	29:61	Sugar Beet Fructooligosaccharides	29:61	Dietary Supplementation with Sugar Beet Fructooligosaccharides and Garlic Residues Promotes Growth of Beneficial Bacteria and Increases Weight Gain in Neonatal Lambs.
32823755	2	74	theme	young	424:428	arg1	ruminants					430:438	young ruminants	424:438	young ruminants	424:438	This microbiota is susceptible to modification by diverse external factors (such as diet) that can lead to long-lasting results when occurring in young ruminants.
32823755	5	75	dep	named	988:992	arg1	all					975:977	all	975:977	all	975:977	We investigated the effect of milk supplementation with a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive"), exerted on preweaned lamb growth and the composition of their fecal microbiota, by using 16S rRNA gene amplicon high-throughput sequencing.
32823755	7	76	theme	Lamb	1254:1257	arg1	microbiota					1265:1274	Lamb fecal microbiota	1254:1274	Lamb fecal microbiota	1254:1274	Lamb fecal microbiota was also influenced by the additive intake, as additive-diet lambs showed lower bacterial diversity and were significantly more abundant in Bifidobacterium, Enterococcus, Lactobacillus and Veillonella.
32823755	0	77	from	Gain	143:146	arg1	Lambs					160:164	Neonatal Lambs	151:164	Neonatal Lambs	151:164	Dietary Supplementation with Sugar Beet Fructooligosaccharides and Garlic Residues Promotes Growth of Beneficial Bacteria and Increases Weight Gain in Neonatal Lambs.
32823755	0	78	theme	Garlic	67:72	arg1	Residues					74:81	Garlic Residues	67:81	Garlic Residues	67:81	Dietary Supplementation with Sugar Beet Fructooligosaccharides and Garlic Residues Promotes Growth of Beneficial Bacteria and Increases Weight Gain in Neonatal Lambs.
32823755	5	79	theme	amplicon	1113:1120	arg1	sequencing					1138:1147	16S rRNA gene amplicon high-throughput sequencing	1099:1147	16S rRNA gene amplicon high-throughput sequencing	1099:1147	We investigated the effect of milk supplementation with a combination of two well-known prebiotics, fructooligosaccharides (FOS) from sugar beet and garlic residues (all together named as "additive"), exerted on preweaned lamb growth and the composition of their fecal microbiota, by using 16S rRNA gene amplicon high-throughput sequencing.
32823755	9	80	theme	garlic	1803:1808	arg1	residues					1810:1817	garlic residues	1803:1817	garlic residues	1803:1817	This study points out the combination of FOS from sugar beet and garlic residues as a potential prebiotic to be used in young ruminants' nutrition in order to improve production yields.
32823755	0	81	with	Supplementation	8:22	arg1	Fructooligosaccharides					40:61	Sugar Beet Fructooligosaccharides	29:61	Sugar Beet Fructooligosaccharides	29:61	Dietary Supplementation with Sugar Beet Fructooligosaccharides and Garlic Residues Promotes Growth of Beneficial Bacteria and Increases Weight Gain in Neonatal Lambs.
32823755	0	81	with	Supplementation	8:22	arg1	Residues					74:81	Garlic Residues	67:81	Garlic Residues	67:81	Dietary Supplementation with Sugar Beet Fructooligosaccharides and Garlic Residues Promotes Growth of Beneficial Bacteria and Increases Weight Gain in Neonatal Lambs.
32823755	3	82	theme	nonabsorbable	512:524	arg1	ingredients					482:492	ingredients	482:492	ingredients nondigestible and nonabsorbable by the host that stimulate the growth of beneficial GIT bacteria	482:589	Dietary supplementation with prebiotics, ingredients nondigestible and nonabsorbable by the host that stimulate the growth of beneficial GIT bacteria, has been applied worldwide as a potential approach in order to improve ruminant health and production yields.
32823755	3	82	theme	nonabsorbable	512:524	arg1	prebiotics					470:479	prebiotics	470:479	prebiotics	470:479	Dietary supplementation with prebiotics, ingredients nondigestible and nonabsorbable by the host that stimulate the growth of beneficial GIT bacteria, has been applied worldwide as a potential approach in order to improve ruminant health and production yields.
32823755	7	83	theme	lower	1350:1354	arg1	diversity					1366:1374	lower bacterial diversity	1350:1374	lower bacterial diversity	1350:1374	Lamb fecal microbiota was also influenced by the additive intake, as additive-diet lambs showed lower bacterial diversity and were significantly more abundant in Bifidobacterium, Enterococcus, Lactobacillus and Veillonella.
32823755	8	84	theme	growth	1595:1600	arg1	promotion					1582:1590	the ruminant, including promotion	1558:1590	the ruminant, including promotion of growth and health status	1558:1618	These bacteria have been previously reported to confer beneficial properties to the ruminant, including promotion of growth and health status, and our results showed that they were strongly linked to the additive intake and the increased weight gain of lambs.
32823755	3	85	theme	ruminant	663:670	arg1	health					672:677	ruminant health and production yields	663:699	health	672:677	Dietary supplementation with prebiotics, ingredients nondigestible and nonabsorbable by the host that stimulate the growth of beneficial GIT bacteria, has been applied worldwide as a potential approach in order to improve ruminant health and production yields.
32823755	3	86	theme	nondigestible	494:506	arg1	ingredients					482:492	ingredients	482:492	ingredients nondigestible and nonabsorbable by the host that stimulate the growth of beneficial GIT bacteria	482:589	Dietary supplementation with prebiotics, ingredients nondigestible and nonabsorbable by the host that stimulate the growth of beneficial GIT bacteria, has been applied worldwide as a potential approach in order to improve ruminant health and production yields.
32823755	3	86	theme	nondigestible	494:506	arg1	prebiotics					470:479	prebiotics	470:479	prebiotics	470:479	Dietary supplementation with prebiotics, ingredients nondigestible and nonabsorbable by the host that stimulate the growth of beneficial GIT bacteria, has been applied worldwide as a potential approach in order to improve ruminant health and production yields.
32823755	1	87	theme	microbiota	232:241	arg1	development					178:188	The proper development	167:188	The proper development of the early gastrointestinal tract (GIT) microbiota	167:241	The proper development of the early gastrointestinal tract (GIT) microbiota is critical for newborn ruminants.
32823755	1	87	theme	microbiota	232:241	arg1	critical					246:253	critical	246:253	critical	246:253	The proper development of the early gastrointestinal tract (GIT) microbiota is critical for newborn ruminants.
32823755	9	88	theme	potential	1824:1832	arg1	prebiotic					1834:1842	a potential prebiotic	1822:1842	a potential prebiotic to be used in young ruminants' nutrition in order to improve production yields	1822:1921	This study points out the combination of FOS from sugar beet and garlic residues as a potential prebiotic to be used in young ruminants' nutrition in order to improve production yields.
32823755	8	89	theme	health	1606:1611	arg1	status					1613:1618	health status	1606:1618	health status	1606:1618	These bacteria have been previously reported to confer beneficial properties to the ruminant, including promotion of growth and health status, and our results showed that they were strongly linked to the additive intake and the increased weight gain of lambs.
34641451	0	0	theme	Dietary	70:76	arg1	Content					84:90	Dietary Fiber Content	70:90	Dietary Fiber Content	70:90	White Chocolate with Resistant Starch: Impact on Physical Properties, Dietary Fiber Content and Sensory Characteristics.
34641451	1	1	theme	dietary	290:296	arg1	fiber					298:302	dietary fiber	290:302	dietary fiber	290:302	Resistant starch (RS) is a part of insoluble dietary fiber, and it could be recognized as a functional food ingredient in some types of confectionery products that lack dietary fiber.
34641451	4	2	theme	dietary	742:748	arg1	content					756:762	dietary fiber content	742:762	dietary fiber content	742:762	The influence of RS on rheological, textural, and thermal properties of the chocolate fat phase was firstly investigated, and then further influence on physical properties, dietary fiber content, and sensory characteristics of enriched white chocolates were investigated.
34641451	6	3	theme	added	1162:1166	arg1	amount					1168:1173	the added amount	1158:1173	the added amount	1158:1173	At the same time, RS increased viscosity and reduced the hardness and volume mean diameter in enriched chocolates in accordance with the added amount.
34641451	4	4	theme	RS	586:587	arg1	influence					573:581	The influence	569:581	The influence of RS on rheological, textural, and thermal properties of the chocolate fat phase	569:663	The influence of RS on rheological, textural, and thermal properties of the chocolate fat phase was firstly investigated, and then further influence on physical properties, dietary fiber content, and sensory characteristics of enriched white chocolates were investigated.
34641451	5	5	contain	had	894:896	arg1	chocolates					883:892	enriched chocolates	874:892	enriched chocolates	874:892	The obtained results showed that enriched chocolates had increased content of total dietary fiber and reduced total fats and protein content in accordance with the added amount of RS.
34641451	5	5	contain	had	894:896	arg2	content					908:914	increased content	898:914	increased content of total dietary fiber	898:937	The obtained results showed that enriched chocolates had increased content of total dietary fiber and reduced total fats and protein content in accordance with the added amount of RS.
34641451	8	6	theme	white	1365:1369	arg1	chocolates					1371:1380	enriched white chocolates	1356:1380	enriched white chocolates	1356:1380	At the same time, RS did not impair the color and sensory characteristics of enriched white chocolates.
34641451	8	7	theme	sensory	1329:1335	arg1	characteristics					1337:1351	sensory characteristics	1329:1351	sensory characteristics	1329:1351	At the same time, RS did not impair the color and sensory characteristics of enriched white chocolates.
34641451	4	8	from	influence	573:581	arg1	properties					627:636	rheological, textural, and thermal properties	592:636	rheological, textural, and thermal properties of the chocolate fat phase	592:663	The influence of RS on rheological, textural, and thermal properties of the chocolate fat phase was firstly investigated, and then further influence on physical properties, dietary fiber content, and sensory characteristics of enriched white chocolates were investigated.
34641451	2	9	from	rich	392:395	arg1	fiber					408:412	dietary fiber	400:412	dietary fiber	400:412	Unlike dark and milk chocolate, white chocolate does not contain fat-free cocoa solids rich in dietary fiber.
34641451	5	10	theme	total	919:923	arg1	fiber					933:937	total dietary fiber	919:937	total dietary fiber	919:937	The obtained results showed that enriched chocolates had increased content of total dietary fiber and reduced total fats and protein content in accordance with the added amount of RS.
34641451	5	11	theme	obtained	845:852	arg1	results					854:860	The obtained results	841:860	The obtained results	841:860	The obtained results showed that enriched chocolates had increased content of total dietary fiber and reduced total fats and protein content in accordance with the added amount of RS.
34641451	7	12	theme	fiber	1272:1276	arg1	content					1253:1259	the content	1249:1259	the content of dietary fiber	1249:1276	RS improved the nutritional composition of white chocolate by increasing the content of dietary fiber.
34641451	1	13	theme	functional	213:222	arg1	ingredient					229:238	a functional food ingredient	211:238	a functional food ingredient in some types of confectionery products that lack dietary fiber	211:302	Resistant starch (RS) is a part of insoluble dietary fiber, and it could be recognized as a functional food ingredient in some types of confectionery products that lack dietary fiber.
34641451	1	13	theme	functional	213:222	arg1	it					185:186	it	185:186	it	185:186	Resistant starch (RS) is a part of insoluble dietary fiber, and it could be recognized as a functional food ingredient in some types of confectionery products that lack dietary fiber.
34641451	0	14	theme	Fiber	78:82	arg1	Content					84:90	Dietary Fiber Content	70:90	Dietary Fiber Content	70:90	White Chocolate with Resistant Starch: Impact on Physical Properties, Dietary Fiber Content and Sensory Characteristics.
34641451	2	15	from	fiber	408:412	arg1	rich					392:395	rich	392:395	rich	392:395	Unlike dark and milk chocolate, white chocolate does not contain fat-free cocoa solids rich in dietary fiber.
34641451	4	16	theme	fiber	750:754	arg1	content					756:762	dietary fiber content	742:762	dietary fiber content	742:762	The influence of RS on rheological, textural, and thermal properties of the chocolate fat phase was firstly investigated, and then further influence on physical properties, dietary fiber content, and sensory characteristics of enriched white chocolates were investigated.
34641451	1	17	theme	food	224:227	arg1	ingredient					229:238	a functional food ingredient	211:238	a functional food ingredient in some types of confectionery products that lack dietary fiber	211:302	Resistant starch (RS) is a part of insoluble dietary fiber, and it could be recognized as a functional food ingredient in some types of confectionery products that lack dietary fiber.
34641451	1	17	theme	food	224:227	arg1	it					185:186	it	185:186	it	185:186	Resistant starch (RS) is a part of insoluble dietary fiber, and it could be recognized as a functional food ingredient in some types of confectionery products that lack dietary fiber.
34641451	0	18	theme	Sensory	96:102	arg1	Characteristics					104:118	Sensory Characteristics	96:118	Sensory Characteristics	96:118	White Chocolate with Resistant Starch: Impact on Physical Properties, Dietary Fiber Content and Sensory Characteristics.
34641451	0	19	from	Impact	39:44	arg1	Content					84:90	Dietary Fiber Content	70:90	Dietary Fiber Content	70:90	White Chocolate with Resistant Starch: Impact on Physical Properties, Dietary Fiber Content and Sensory Characteristics.
34641451	0	19	from	Impact	39:44	arg1	Characteristics					104:118	Sensory Characteristics	96:118	Sensory Characteristics	96:118	White Chocolate with Resistant Starch: Impact on Physical Properties, Dietary Fiber Content and Sensory Characteristics.
34641451	0	19	from	Impact	39:44	arg1	Properties					58:67	Physical Properties	49:67	Physical Properties	49:67	White Chocolate with Resistant Starch: Impact on Physical Properties, Dietary Fiber Content and Sensory Characteristics.
34641451	4	20	theme	fat	655:657	arg1	phase					659:663	the chocolate fat phase	641:663	the chocolate fat phase	641:663	The influence of RS on rheological, textural, and thermal properties of the chocolate fat phase was firstly investigated, and then further influence on physical properties, dietary fiber content, and sensory characteristics of enriched white chocolates were investigated.
34641451	7	21	theme	nutritional	1192:1202	arg1	composition					1204:1214	the nutritional composition	1188:1214	the nutritional composition of white chocolate	1188:1233	RS improved the nutritional composition of white chocolate by increasing the content of dietary fiber.
34641451	6	22	theme	enriched	1119:1126	arg1	chocolates					1128:1137	enriched chocolates	1119:1137	enriched chocolates	1119:1137	At the same time, RS increased viscosity and reduced the hardness and volume mean diameter in enriched chocolates in accordance with the added amount.
34641451	4	23	theme	chocolate	645:653	arg1	phase					659:663	the chocolate fat phase	641:663	the chocolate fat phase	641:663	The influence of RS on rheological, textural, and thermal properties of the chocolate fat phase was firstly investigated, and then further influence on physical properties, dietary fiber content, and sensory characteristics of enriched white chocolates were investigated.
34641451	4	24	from	influence	708:716	arg1	properties					730:739	physical properties	721:739	physical properties	721:739	The influence of RS on rheological, textural, and thermal properties of the chocolate fat phase was firstly investigated, and then further influence on physical properties, dietary fiber content, and sensory characteristics of enriched white chocolates were investigated.
34641451	4	24	from	influence	708:716	arg1	characteristics					777:791	sensory characteristics	769:791	sensory characteristics	769:791	The influence of RS on rheological, textural, and thermal properties of the chocolate fat phase was firstly investigated, and then further influence on physical properties, dietary fiber content, and sensory characteristics of enriched white chocolates were investigated.
34641451	4	24	from	influence	708:716	arg1	content					756:762	dietary fiber content	742:762	dietary fiber content	742:762	The influence of RS on rheological, textural, and thermal properties of the chocolate fat phase was firstly investigated, and then further influence on physical properties, dietary fiber content, and sensory characteristics of enriched white chocolates were investigated.
34641451	1	25	from	ingredient	229:238	arg1	types					248:252	some types	243:252	some types of confectionery products that lack dietary fiber	243:302	Resistant starch (RS) is a part of insoluble dietary fiber, and it could be recognized as a functional food ingredient in some types of confectionery products that lack dietary fiber.
34641451	6	26	theme	same	1032:1035	arg1	time					1037:1040	the same time	1028:1040	the same time	1028:1040	At the same time, RS increased viscosity and reduced the hardness and volume mean diameter in enriched chocolates in accordance with the added amount.
34641451	8	27	theme	chocolates	1371:1380	arg1	color					1319:1323	color	1319:1323	color	1319:1323	At the same time, RS did not impair the color and sensory characteristics of enriched white chocolates.
34641451	8	27	theme	chocolates	1371:1380	arg1	characteristics					1337:1351	sensory characteristics	1329:1351	sensory characteristics	1329:1351	At the same time, RS did not impair the color and sensory characteristics of enriched white chocolates.
34641451	2	28	theme	rich	392:395	arg1	solids					385:390	fat-free cocoa solids	370:390	fat-free cocoa solids rich in dietary fiber	370:412	Unlike dark and milk chocolate, white chocolate does not contain fat-free cocoa solids rich in dietary fiber.
34641451	4	29	theme	physical	721:728	arg1	properties					730:739	physical properties	721:739	physical properties	721:739	The influence of RS on rheological, textural, and thermal properties of the chocolate fat phase was firstly investigated, and then further influence on physical properties, dietary fiber content, and sensory characteristics of enriched white chocolates were investigated.
34641451	8	30	theme	enriched	1356:1363	arg1	chocolates					1371:1380	enriched white chocolates	1356:1380	enriched white chocolates	1356:1380	At the same time, RS did not impair the color and sensory characteristics of enriched white chocolates.
34641451	3	31	theme	chocolate	463:471	arg1	%					438:438	5%	437:438	5%	437:438	In the present study, 5%, 10%, and 15% of white chocolate were substituted with RS in order to improve the nutritional value of enriched white chocolate.
34641451	3	31	theme	chocolate	463:471	arg1	%					443:443	10%	441:443	10%	441:443	In the present study, 5%, 10%, and 15% of white chocolate were substituted with RS in order to improve the nutritional value of enriched white chocolate.
34641451	3	31	theme	chocolate	463:471	arg1	%					452:452	15%	450:452	15%	450:452	In the present study, 5%, 10%, and 15% of white chocolate were substituted with RS in order to improve the nutritional value of enriched white chocolate.
34641451	3	31	theme	chocolate	463:471	arg1	chocolate					463:471	chocolate	463:471	chocolate	463:471	In the present study, 5%, 10%, and 15% of white chocolate were substituted with RS in order to improve the nutritional value of enriched white chocolate.
34641451	1	32	theme	insoluble	156:164	arg1	fiber					174:178	insoluble dietary fiber	156:178	insoluble dietary fiber	156:178	Resistant starch (RS) is a part of insoluble dietary fiber, and it could be recognized as a functional food ingredient in some types of confectionery products that lack dietary fiber.
34641451	0	33	theme	Resistant	21:29	arg1	Starch					31:36	Resistant Starch	21:36	Resistant Starch	21:36	White Chocolate with Resistant Starch: Impact on Physical Properties, Dietary Fiber Content and Sensory Characteristics.
34641451	4	34	theme	enriched	796:803	arg1	chocolates					811:820	enriched white chocolates	796:820	enriched white chocolates	796:820	The influence of RS on rheological, textural, and thermal properties of the chocolate fat phase was firstly investigated, and then further influence on physical properties, dietary fiber content, and sensory characteristics of enriched white chocolates were investigated.
34641451	5	35	theme	fiber	933:937	arg1	content					908:914	increased content	898:914	increased content of total dietary fiber	898:937	The obtained results showed that enriched chocolates had increased content of total dietary fiber and reduced total fats and protein content in accordance with the added amount of RS.
34641451	2	36	theme	fat-free	370:377	arg1	solids					385:390	fat-free cocoa solids	370:390	fat-free cocoa solids rich in dietary fiber	370:412	Unlike dark and milk chocolate, white chocolate does not contain fat-free cocoa solids rich in dietary fiber.
34641451	2	37	theme	cocoa	379:383	arg1	solids					385:390	fat-free cocoa solids	370:390	fat-free cocoa solids rich in dietary fiber	370:412	Unlike dark and milk chocolate, white chocolate does not contain fat-free cocoa solids rich in dietary fiber.
34641451	8	38	dep	color	1319:1323	arg1	the					1315:1317	the	1315:1317	the	1315:1317	At the same time, RS did not impair the color and sensory characteristics of enriched white chocolates.
34641451	4	39	theme	thermal	619:625	arg1	properties					627:636	rheological, textural, and thermal properties	592:636	rheological, textural, and thermal properties of the chocolate fat phase	592:663	The influence of RS on rheological, textural, and thermal properties of the chocolate fat phase was firstly investigated, and then further influence on physical properties, dietary fiber content, and sensory characteristics of enriched white chocolates were investigated.
34641451	1	40	theme	fiber	174:178	arg1	starch					131:136	Resistant starch	121:136	Resistant starch (RS)	121:141	Resistant starch (RS) is a part of insoluble dietary fiber, and it could be recognized as a functional food ingredient in some types of confectionery products that lack dietary fiber.
34641451	1	40	theme	fiber	174:178	arg1	part					148:151	a part	146:151	a part of insoluble dietary fiber	146:178	Resistant starch (RS) is a part of insoluble dietary fiber, and it could be recognized as a functional food ingredient in some types of confectionery products that lack dietary fiber.
34641451	4	41	theme	chocolates	811:820	arg1	influence					708:716	further influence	700:716	further influence on physical properties, dietary fiber content, and sensory characteristics of enriched white chocolates	700:820	The influence of RS on rheological, textural, and thermal properties of the chocolate fat phase was firstly investigated, and then further influence on physical properties, dietary fiber content, and sensory characteristics of enriched white chocolates were investigated.
34641451	6	42	theme	mean	1102:1105	arg1	diameter					1107:1114	mean diameter	1102:1114	mean diameter	1102:1114	At the same time, RS increased viscosity and reduced the hardness and volume mean diameter in enriched chocolates in accordance with the added amount.
34641451	7	43	theme	dietary	1264:1270	arg1	fiber					1272:1276	dietary fiber	1264:1276	dietary fiber	1264:1276	RS improved the nutritional composition of white chocolate by increasing the content of dietary fiber.
34641451	4	44	theme	white	805:809	arg1	chocolates					811:820	enriched white chocolates	796:820	enriched white chocolates	796:820	The influence of RS on rheological, textural, and thermal properties of the chocolate fat phase was firstly investigated, and then further influence on physical properties, dietary fiber content, and sensory characteristics of enriched white chocolates were investigated.
34641451	5	45	theme	protein	966:972	arg1	content					974:980	protein content	966:980	protein content	966:980	The obtained results showed that enriched chocolates had increased content of total dietary fiber and reduced total fats and protein content in accordance with the added amount of RS.
34641451	0	46	dep	Impact	39:44	arg1	Chocolate					6:14	Chocolate	6:14	Chocolate	6:14	White Chocolate with Resistant Starch: Impact on Physical Properties, Dietary Fiber Content and Sensory Characteristics.
34641451	1	47	theme	confectionery	257:269	arg1	products					271:278	confectionery products	257:278	confectionery products that lack dietary fiber	257:302	Resistant starch (RS) is a part of insoluble dietary fiber, and it could be recognized as a functional food ingredient in some types of confectionery products that lack dietary fiber.
34641451	3	48	theme	nutritional	522:532	arg1	value					534:538	the nutritional value	518:538	the nutritional value of enriched white chocolate	518:566	In the present study, 5%, 10%, and 15% of white chocolate were substituted with RS in order to improve the nutritional value of enriched white chocolate.
34641451	4	49	theme	further	700:706	arg1	influence					708:716	further influence	700:716	further influence on physical properties, dietary fiber content, and sensory characteristics of enriched white chocolates	700:820	The influence of RS on rheological, textural, and thermal properties of the chocolate fat phase was firstly investigated, and then further influence on physical properties, dietary fiber content, and sensory characteristics of enriched white chocolates were investigated.
34641451	4	50	theme	rheological	592:602	arg1	properties					627:636	rheological, textural, and thermal properties	592:636	rheological, textural, and thermal properties of the chocolate fat phase	592:663	The influence of RS on rheological, textural, and thermal properties of the chocolate fat phase was firstly investigated, and then further influence on physical properties, dietary fiber content, and sensory characteristics of enriched white chocolates were investigated.
34641451	2	51	theme	dietary	400:406	arg1	fiber					408:412	dietary fiber	400:412	dietary fiber	400:412	Unlike dark and milk chocolate, white chocolate does not contain fat-free cocoa solids rich in dietary fiber.
34641451	1	52	theme	products	271:278	arg1	types					248:252	some types	243:252	some types of confectionery products that lack dietary fiber	243:302	Resistant starch (RS) is a part of insoluble dietary fiber, and it could be recognized as a functional food ingredient in some types of confectionery products that lack dietary fiber.
34641451	3	53	theme	chocolate	558:566	arg1	value					534:538	the nutritional value	518:538	the nutritional value of enriched white chocolate	518:566	In the present study, 5%, 10%, and 15% of white chocolate were substituted with RS in order to improve the nutritional value of enriched white chocolate.
34641451	5	54	theme	dietary	925:931	arg1	fiber					933:937	total dietary fiber	919:937	total dietary fiber	919:937	The obtained results showed that enriched chocolates had increased content of total dietary fiber and reduced total fats and protein content in accordance with the added amount of RS.
34641451	0	55	with	Chocolate	6:14	arg1	Starch					31:36	Resistant Starch	21:36	Resistant Starch	21:36	White Chocolate with Resistant Starch: Impact on Physical Properties, Dietary Fiber Content and Sensory Characteristics.
34641451	8	56	theme	same	1286:1289	arg1	time					1291:1294	the same time	1282:1294	the same time	1282:1294	At the same time, RS did not impair the color and sensory characteristics of enriched white chocolates.
34641451	7	57	theme	chocolate	1225:1233	arg1	composition					1204:1214	the nutritional composition	1188:1214	the nutritional composition of white chocolate	1188:1233	RS improved the nutritional composition of white chocolate by increasing the content of dietary fiber.
34641451	5	58	theme	enriched	874:881	arg1	chocolates					883:892	enriched chocolates	874:892	enriched chocolates	874:892	The obtained results showed that enriched chocolates had increased content of total dietary fiber and reduced total fats and protein content in accordance with the added amount of RS.
34641451	1	59	theme	Resistant	121:129	arg1	RS					139:140	RS	139:140	RS	139:140	Resistant starch (RS) is a part of insoluble dietary fiber, and it could be recognized as a functional food ingredient in some types of confectionery products that lack dietary fiber.
34641451	1	59	theme	Resistant	121:129	arg1	starch					131:136	Resistant starch	121:136	Resistant starch (RS)	121:141	Resistant starch (RS) is a part of insoluble dietary fiber, and it could be recognized as a functional food ingredient in some types of confectionery products that lack dietary fiber.
34641451	1	59	theme	Resistant	121:129	arg1	part					148:151	a part	146:151	a part of insoluble dietary fiber	146:178	Resistant starch (RS) is a part of insoluble dietary fiber, and it could be recognized as a functional food ingredient in some types of confectionery products that lack dietary fiber.
34641451	6	60	from	hardness	1082:1089	arg1	chocolates					1128:1137	enriched chocolates	1119:1137	enriched chocolates	1119:1137	At the same time, RS increased viscosity and reduced the hardness and volume mean diameter in enriched chocolates in accordance with the added amount.
34641451	4	61	theme	sensory	769:775	arg1	characteristics					777:791	sensory characteristics	769:791	sensory characteristics	769:791	The influence of RS on rheological, textural, and thermal properties of the chocolate fat phase was firstly investigated, and then further influence on physical properties, dietary fiber content, and sensory characteristics of enriched white chocolates were investigated.
34641451	2	62	contain	contain	362:368	arg1	chocolate					343:351	chocolate	343:351	chocolate	343:351	Unlike dark and milk chocolate, white chocolate does not contain fat-free cocoa solids rich in dietary fiber.
34641451	2	62	contain	contain	362:368	arg2	solids					385:390	fat-free cocoa solids	370:390	fat-free cocoa solids rich in dietary fiber	370:412	Unlike dark and milk chocolate, white chocolate does not contain fat-free cocoa solids rich in dietary fiber.
34641451	4	63	theme	phase	659:663	arg1	properties					627:636	rheological, textural, and thermal properties	592:636	rheological, textural, and thermal properties of the chocolate fat phase	592:663	The influence of RS on rheological, textural, and thermal properties of the chocolate fat phase was firstly investigated, and then further influence on physical properties, dietary fiber content, and sensory characteristics of enriched white chocolates were investigated.
34641451	3	64	theme	present	422:428	arg1	study					430:434	the present study	418:434	the present study	418:434	In the present study, 5%, 10%, and 15% of white chocolate were substituted with RS in order to improve the nutritional value of enriched white chocolate.
34641451	4	65	theme	textural	605:612	arg1	properties					627:636	rheological, textural, and thermal properties	592:636	rheological, textural, and thermal properties of the chocolate fat phase	592:663	The influence of RS on rheological, textural, and thermal properties of the chocolate fat phase was firstly investigated, and then further influence on physical properties, dietary fiber content, and sensory characteristics of enriched white chocolates were investigated.
34641451	5	66	theme	total	951:955	arg1	fats					957:960	total fats	951:960	total fats	951:960	The obtained results showed that enriched chocolates had increased content of total dietary fiber and reduced total fats and protein content in accordance with the added amount of RS.
34641451	5	67	theme	RS	1021:1022	arg1	amount					1011:1016	the added amount	1001:1016	the added amount of RS	1001:1022	The obtained results showed that enriched chocolates had increased content of total dietary fiber and reduced total fats and protein content in accordance with the added amount of RS.
34641451	5	67	theme	RS	1021:1022	arg1	RS					1021:1022	RS	1021:1022	RS	1021:1022	The obtained results showed that enriched chocolates had increased content of total dietary fiber and reduced total fats and protein content in accordance with the added amount of RS.
34641451	1	68	theme	dietary	166:172	arg1	fiber					174:178	insoluble dietary fiber	156:178	insoluble dietary fiber	156:178	Resistant starch (RS) is a part of insoluble dietary fiber, and it could be recognized as a functional food ingredient in some types of confectionery products that lack dietary fiber.
34641451	6	69	from	volume	1095:1100	arg1	chocolates					1128:1137	enriched chocolates	1119:1137	enriched chocolates	1119:1137	At the same time, RS increased viscosity and reduced the hardness and volume mean diameter in enriched chocolates in accordance with the added amount.
34641451	0	70	theme	Physical	49:56	arg1	Properties					58:67	Physical Properties	49:67	Physical Properties	49:67	White Chocolate with Resistant Starch: Impact on Physical Properties, Dietary Fiber Content and Sensory Characteristics.
34641451	5	71	theme	added	1005:1009	arg1	amount					1011:1016	the added amount	1001:1016	the added amount of RS	1001:1022	The obtained results showed that enriched chocolates had increased content of total dietary fiber and reduced total fats and protein content in accordance with the added amount of RS.
34641451	5	71	theme	added	1005:1009	arg1	RS					1021:1022	RS	1021:1022	RS	1021:1022	The obtained results showed that enriched chocolates had increased content of total dietary fiber and reduced total fats and protein content in accordance with the added amount of RS.
34641451	5	72	theme	increased	898:906	arg1	content					908:914	increased content	898:914	increased content of total dietary fiber	898:937	The obtained results showed that enriched chocolates had increased content of total dietary fiber and reduced total fats and protein content in accordance with the added amount of RS.
34641451	6	73	dep	hardness	1082:1089	arg1	diameter					1107:1114	mean diameter	1102:1114	mean diameter	1102:1114	At the same time, RS increased viscosity and reduced the hardness and volume mean diameter in enriched chocolates in accordance with the added amount.
34641451	6	73	dep	hardness	1082:1089	arg1	the					1078:1080	the	1078:1080	the	1078:1080	At the same time, RS increased viscosity and reduced the hardness and volume mean diameter in enriched chocolates in accordance with the added amount.
32156109	2	0	from	acid	591:594	arg1	pot					603:605	one pot	599:605	one pot	599:605	In this study, a robust and easy-to-use multienzymatic cascade reaction system of coimmobilized GA@GOx hybrid nanoflowers with a specific spatial distribution of enzymes by compartmentalization was constructed and applied to catalyze starch to gluconic acid in one pot.
32156109	6	1	theme	substrate	1214:1222	arg1	channeling					1224:1233	the substrate channeling	1210:1233	the substrate channeling	1210:1233	Based on the lower Km, the GA@GOx hybrid nanoflowers with compartmentalization had a better effect of the substrate channeling on the catalytic efficiency.
32156109	5	2	dep	morphology	1006:1015	arg1	the					1002:1004	the	1002:1004	the	1002:1004	In addition, the morphology, composition, and crystallization of the GA@GOx hybrid nanoflowers had been fully studied.
32156109	6	3	theme	lower	1121:1125	arg1	Km					1127:1128	the lower Km	1117:1128	the lower Km	1117:1128	Based on the lower Km, the GA@GOx hybrid nanoflowers with compartmentalization had a better effect of the substrate channeling on the catalytic efficiency.
32156109	3	4	theme	self-assembly	712:724	arg1	mechanism					726:734	a self-assembly mechanism	710:734	a self-assembly mechanism	710:734	In the preparation processes, the glucose oxidase (GOx) hybrid nanoflowers were first synthesized via a self-assembly mechanism, and then, glucoamylase (GA) was adsorbed on the surface of GOx hybrid nanoflowers through the interaction of Cu2+ and amino acids of GA.
32156109	6	5	theme	better	1193:1198	arg1	effect					1200:1205	a better effect	1191:1205	a better effect of the substrate channeling on the catalytic efficiency	1191:1261	Based on the lower Km, the GA@GOx hybrid nanoflowers with compartmentalization had a better effect of the substrate channeling on the catalytic efficiency.
32156109	7	6	theme	GOx	1335:1337	arg1	nanoflowers					1346:1356	the GA@GOx hybrid nanoflowers	1328:1356	the GA@GOx hybrid nanoflowers	1328:1356	The final results indicated that the overall enzyme activity of the GA@GOx hybrid nanoflowers increased by 1.5 times, and the conversion efficiency was 92.12% within 80 min significantly superior to the free multienzyme system, which showed the outstanding conversion of starch into gluconic acid in one pot.
32156109	1	7	theme	starch	208:213	arg1	conversion					194:203	the rapid conversion	184:203	the rapid conversion of starch	184:213	Introducing an efficient method for the rapid conversion of starch into gluconic acid is desirable to solve the current problems existing in traditional gluconic acid preparation processes.
32156109	4	8	theme	reaction	913:920	arg1	parameters					922:931	reaction parameters	913:931	reaction parameters	913:931	The optimum preparation conditions and reaction parameters of the GA@GOx hybrid nanoflowers had been investigated.
32156109	1	9	theme	gluconic	220:227	arg1	acid					229:232	gluconic acid	220:232	gluconic acid	220:232	Introducing an efficient method for the rapid conversion of starch into gluconic acid is desirable to solve the current problems existing in traditional gluconic acid preparation processes.
32156109	7	10	theme	enzyme	1309:1314	arg1	activity					1316:1323	the overall enzyme activity	1297:1323	the overall enzyme activity of the GA@GOx hybrid nanoflowers	1297:1356	The final results indicated that the overall enzyme activity of the GA@GOx hybrid nanoflowers increased by 1.5 times, and the conversion efficiency was 92.12% within 80 min significantly superior to the free multienzyme system, which showed the outstanding conversion of starch into gluconic acid in one pot.
32156109	6	11	theme	catalytic	1242:1250	arg1	efficiency					1252:1261	the catalytic efficiency	1238:1261	the catalytic efficiency	1238:1261	Based on the lower Km, the GA@GOx hybrid nanoflowers with compartmentalization had a better effect of the substrate channeling on the catalytic efficiency.
32156109	4	12	theme	optimum	878:884	arg1	conditions					898:907	The optimum preparation conditions	874:907	The optimum preparation conditions	874:907	The optimum preparation conditions and reaction parameters of the GA@GOx hybrid nanoflowers had been investigated.
32156109	6	13	theme	GOx	1138:1140	arg1	nanoflowers					1149:1159	the GA@GOx hybrid nanoflowers	1131:1159	the GA@GOx hybrid nanoflowers with compartmentalization	1131:1185	Based on the lower Km, the GA@GOx hybrid nanoflowers with compartmentalization had a better effect of the substrate channeling on the catalytic efficiency.
32156109	7	14	theme	outstanding	1509:1519	arg1	conversion					1521:1530	the outstanding conversion	1505:1530	the outstanding conversion of starch into gluconic acid in one pot	1505:1570	The final results indicated that the overall enzyme activity of the GA@GOx hybrid nanoflowers increased by 1.5 times, and the conversion efficiency was 92.12% within 80 min significantly superior to the free multienzyme system, which showed the outstanding conversion of starch into gluconic acid in one pot.
32156109	4	15	theme	GOx	943:945	arg1	nanoflowers					954:964	the GA@GOx hybrid nanoflowers	936:964	the GA@GOx hybrid nanoflowers	936:964	The optimum preparation conditions and reaction parameters of the GA@GOx hybrid nanoflowers had been investigated.
32156109	6	16	theme	GA	1135:1136	arg1	nanoflowers					1149:1159	the GA@GOx hybrid nanoflowers	1131:1159	the GA@GOx hybrid nanoflowers with compartmentalization	1131:1185	Based on the lower Km, the GA@GOx hybrid nanoflowers with compartmentalization had a better effect of the substrate channeling on the catalytic efficiency.
32156109	6	17	from	effect	1200:1205	arg1	efficiency					1252:1261	the catalytic efficiency	1238:1261	the catalytic efficiency	1238:1261	Based on the lower Km, the GA@GOx hybrid nanoflowers with compartmentalization had a better effect of the substrate channeling on the catalytic efficiency.
32156109	5	18	theme	nanoflowers	1072:1082	arg1	composition					1018:1028	composition	1018:1028	composition	1018:1028	In addition, the morphology, composition, and crystallization of the GA@GOx hybrid nanoflowers had been fully studied.
32156109	5	18	theme	nanoflowers	1072:1082	arg1	crystallization					1035:1049	crystallization	1035:1049	crystallization	1035:1049	In addition, the morphology, composition, and crystallization of the GA@GOx hybrid nanoflowers had been fully studied.
32156109	5	18	theme	nanoflowers	1072:1082	arg1	morphology					1006:1015	morphology	1006:1015	morphology	1006:1015	In addition, the morphology, composition, and crystallization of the GA@GOx hybrid nanoflowers had been fully studied.
32156109	2	19	theme	enzymes	500:506	arg1	distribution					484:495	a specific spatial distribution	465:495	a specific spatial distribution of enzymes by compartmentalization	465:530	In this study, a robust and easy-to-use multienzymatic cascade reaction system of coimmobilized GA@GOx hybrid nanoflowers with a specific spatial distribution of enzymes by compartmentalization was constructed and applied to catalyze starch to gluconic acid in one pot.
32156109	3	20	theme	nanoflowers	807:817	arg1	surface					785:791	the surface	781:791	the surface of GOx hybrid nanoflowers	781:817	In the preparation processes, the glucose oxidase (GOx) hybrid nanoflowers were first synthesized via a self-assembly mechanism, and then, glucoamylase (GA) was adsorbed on the surface of GOx hybrid nanoflowers through the interaction of Cu2+ and amino acids of GA.
32156109	0	21	theme	Nanoflowers	81:91	arg1	System					50:55	a Multienzymatic Cascade Reaction System	16:55	a Multienzymatic Cascade Reaction System of Coimmobilized Hybrid Nanoflowers for Efficient Conversion of Starch into Gluconic Acid	16:145	Construction of a Multienzymatic Cascade Reaction System of Coimmobilized Hybrid Nanoflowers for Efficient Conversion of Starch into Gluconic Acid.
32156109	6	22	theme	@	1137:1137	arg1	nanoflowers					1149:1159	the GA@GOx hybrid nanoflowers	1131:1159	the GA@GOx hybrid nanoflowers with compartmentalization	1131:1185	Based on the lower Km, the GA@GOx hybrid nanoflowers with compartmentalization had a better effect of the substrate channeling on the catalytic efficiency.
32156109	3	23	theme	GOx	796:798	arg1	nanoflowers					807:817	GOx hybrid nanoflowers	796:817	GOx hybrid nanoflowers	796:817	In the preparation processes, the glucose oxidase (GOx) hybrid nanoflowers were first synthesized via a self-assembly mechanism, and then, glucoamylase (GA) was adsorbed on the surface of GOx hybrid nanoflowers through the interaction of Cu2+ and amino acids of GA.
32156109	0	24	theme	Efficient	97:105	arg1	Conversion					107:116	Efficient Conversion	97:116	Efficient Conversion of Starch into Gluconic Acid	97:145	Construction of a Multienzymatic Cascade Reaction System of Coimmobilized Hybrid Nanoflowers for Efficient Conversion of Starch into Gluconic Acid.
32156109	7	25	theme	multienzyme	1472:1482	arg1	system					1484:1489	the free multienzyme system	1463:1489	the free multienzyme system	1463:1489	The final results indicated that the overall enzyme activity of the GA@GOx hybrid nanoflowers increased by 1.5 times, and the conversion efficiency was 92.12% within 80 min significantly superior to the free multienzyme system, which showed the outstanding conversion of starch into gluconic acid in one pot.
32156109	5	26	theme	GOx	1061:1063	arg1	nanoflowers					1072:1082	the GA@GOx hybrid nanoflowers	1054:1082	the GA@GOx hybrid nanoflowers	1054:1082	In addition, the morphology, composition, and crystallization of the GA@GOx hybrid nanoflowers had been fully studied.
32156109	7	27	from	acid	1556:1559	arg1	pot					1568:1570	one pot	1564:1570	one pot	1564:1570	The final results indicated that the overall enzyme activity of the GA@GOx hybrid nanoflowers increased by 1.5 times, and the conversion efficiency was 92.12% within 80 min significantly superior to the free multienzyme system, which showed the outstanding conversion of starch into gluconic acid in one pot.
32156109	3	28	theme	GA	870:871	arg1	acids					861:865	amino acids	855:865	amino acids of GA	855:871	In the preparation processes, the glucose oxidase (GOx) hybrid nanoflowers were first synthesized via a self-assembly mechanism, and then, glucoamylase (GA) was adsorbed on the surface of GOx hybrid nanoflowers through the interaction of Cu2+ and amino acids of GA.
32156109	3	28	theme	GA	870:871	arg1	interaction					831:841	the interaction	827:841	the interaction of Cu2+	827:849	In the preparation processes, the glucose oxidase (GOx) hybrid nanoflowers were first synthesized via a self-assembly mechanism, and then, glucoamylase (GA) was adsorbed on the surface of GOx hybrid nanoflowers through the interaction of Cu2+ and amino acids of GA.
32156109	2	29	theme	cascade	393:399	arg1	system					410:415	a robust and easy-to-use multienzymatic cascade reaction system	353:415	a robust and easy-to-use multienzymatic cascade reaction system of coimmobilized GA@GOx hybrid nanoflowers with a specific spatial distribution of enzymes by compartmentalization	353:530	In this study, a robust and easy-to-use multienzymatic cascade reaction system of coimmobilized GA@GOx hybrid nanoflowers with a specific spatial distribution of enzymes by compartmentalization was constructed and applied to catalyze starch to gluconic acid in one pot.
32156109	6	30	contain	had	1187:1189	arg1	nanoflowers					1149:1159	the GA@GOx hybrid nanoflowers	1131:1159	the GA@GOx hybrid nanoflowers with compartmentalization	1131:1185	Based on the lower Km, the GA@GOx hybrid nanoflowers with compartmentalization had a better effect of the substrate channeling on the catalytic efficiency.
32156109	6	30	contain	had	1187:1189	arg2	effect					1200:1205	a better effect	1191:1205	a better effect of the substrate channeling on the catalytic efficiency	1191:1261	Based on the lower Km, the GA@GOx hybrid nanoflowers with compartmentalization had a better effect of the substrate channeling on the catalytic efficiency.
32156109	2	31	theme	hybrid	441:446	arg1	nanoflowers					448:458	coimmobilized GA@GOx hybrid nanoflowers	420:458	coimmobilized GA@GOx hybrid nanoflowers with a specific spatial distribution of enzymes by compartmentalization	420:530	In this study, a robust and easy-to-use multienzymatic cascade reaction system of coimmobilized GA@GOx hybrid nanoflowers with a specific spatial distribution of enzymes by compartmentalization was constructed and applied to catalyze starch to gluconic acid in one pot.
32156109	2	32	theme	@	436:436	arg1	nanoflowers					448:458	coimmobilized GA@GOx hybrid nanoflowers	420:458	coimmobilized GA@GOx hybrid nanoflowers with a specific spatial distribution of enzymes by compartmentalization	420:530	In this study, a robust and easy-to-use multienzymatic cascade reaction system of coimmobilized GA@GOx hybrid nanoflowers with a specific spatial distribution of enzymes by compartmentalization was constructed and applied to catalyze starch to gluconic acid in one pot.
32156109	1	33	theme	traditional	289:299	arg1	acid					310:313	traditional gluconic acid	289:313	traditional gluconic acid preparation processes	289:335	Introducing an efficient method for the rapid conversion of starch into gluconic acid is desirable to solve the current problems existing in traditional gluconic acid preparation processes.
32156109	7	34	theme	nanoflowers	1346:1356	arg1	activity					1316:1323	the overall enzyme activity	1297:1323	the overall enzyme activity of the GA@GOx hybrid nanoflowers	1297:1356	The final results indicated that the overall enzyme activity of the GA@GOx hybrid nanoflowers increased by 1.5 times, and the conversion efficiency was 92.12% within 80 min significantly superior to the free multienzyme system, which showed the outstanding conversion of starch into gluconic acid in one pot.
32156109	0	35	theme	Coimmobilized	60:72	arg1	Nanoflowers					81:91	Coimmobilized Hybrid Nanoflowers	60:91	Coimmobilized Hybrid Nanoflowers	60:91	Construction of a Multienzymatic Cascade Reaction System of Coimmobilized Hybrid Nanoflowers for Efficient Conversion of Starch into Gluconic Acid.
32156109	2	36	theme	coimmobilized	420:432	arg1	nanoflowers					448:458	coimmobilized GA@GOx hybrid nanoflowers	420:458	coimmobilized GA@GOx hybrid nanoflowers with a specific spatial distribution of enzymes by compartmentalization	420:530	In this study, a robust and easy-to-use multienzymatic cascade reaction system of coimmobilized GA@GOx hybrid nanoflowers with a specific spatial distribution of enzymes by compartmentalization was constructed and applied to catalyze starch to gluconic acid in one pot.
32156109	0	37	theme	Cascade	33:39	arg1	System					50:55	a Multienzymatic Cascade Reaction System	16:55	a Multienzymatic Cascade Reaction System of Coimmobilized Hybrid Nanoflowers for Efficient Conversion of Starch into Gluconic Acid	16:145	Construction of a Multienzymatic Cascade Reaction System of Coimmobilized Hybrid Nanoflowers for Efficient Conversion of Starch into Gluconic Acid.
32156109	1	38	theme	acid	310:313	arg1	processes					327:335	traditional gluconic acid preparation processes	289:335	traditional gluconic acid preparation processes	289:335	Introducing an efficient method for the rapid conversion of starch into gluconic acid is desirable to solve the current problems existing in traditional gluconic acid preparation processes.
32156109	2	39	theme	specific	467:474	arg1	distribution					484:495	a specific spatial distribution	465:495	a specific spatial distribution of enzymes by compartmentalization	465:530	In this study, a robust and easy-to-use multienzymatic cascade reaction system of coimmobilized GA@GOx hybrid nanoflowers with a specific spatial distribution of enzymes by compartmentalization was constructed and applied to catalyze starch to gluconic acid in one pot.
32156109	3	40	theme	oxidase	650:656	arg1	nanoflowers					671:681	the glucose oxidase (GOx) hybrid nanoflowers	638:681	the glucose oxidase (GOx) hybrid nanoflowers	638:681	In the preparation processes, the glucose oxidase (GOx) hybrid nanoflowers were first synthesized via a self-assembly mechanism, and then, glucoamylase (GA) was adsorbed on the surface of GOx hybrid nanoflowers through the interaction of Cu2+ and amino acids of GA.
32156109	0	41	theme	System	50:55	arg1	Construction					0:11	Construction	0:11	Construction of a Multienzymatic Cascade Reaction System of Coimmobilized Hybrid Nanoflowers for Efficient Conversion of Starch into Gluconic Acid.	0:146	Construction of a Multienzymatic Cascade Reaction System of Coimmobilized Hybrid Nanoflowers for Efficient Conversion of Starch into Gluconic Acid.
32156109	3	42	theme	hybrid	664:669	arg1	nanoflowers					671:681	the glucose oxidase (GOx) hybrid nanoflowers	638:681	the glucose oxidase (GOx) hybrid nanoflowers	638:681	In the preparation processes, the glucose oxidase (GOx) hybrid nanoflowers were first synthesized via a self-assembly mechanism, and then, glucoamylase (GA) was adsorbed on the surface of GOx hybrid nanoflowers through the interaction of Cu2+ and amino acids of GA.
32156109	2	43	theme	easy-to-use	366:376	arg1	system					410:415	a robust and easy-to-use multienzymatic cascade reaction system	353:415	a robust and easy-to-use multienzymatic cascade reaction system of coimmobilized GA@GOx hybrid nanoflowers with a specific spatial distribution of enzymes by compartmentalization	353:530	In this study, a robust and easy-to-use multienzymatic cascade reaction system of coimmobilized GA@GOx hybrid nanoflowers with a specific spatial distribution of enzymes by compartmentalization was constructed and applied to catalyze starch to gluconic acid in one pot.
32156109	4	44	theme	nanoflowers	954:964	arg1	conditions					898:907	The optimum preparation conditions	874:907	The optimum preparation conditions	874:907	The optimum preparation conditions and reaction parameters of the GA@GOx hybrid nanoflowers had been investigated.
32156109	4	44	theme	nanoflowers	954:964	arg1	parameters					922:931	reaction parameters	913:931	reaction parameters	913:931	The optimum preparation conditions and reaction parameters of the GA@GOx hybrid nanoflowers had been investigated.
32156109	6	45	theme	channeling	1224:1233	arg1	effect					1200:1205	a better effect	1191:1205	a better effect of the substrate channeling on the catalytic efficiency	1191:1261	Based on the lower Km, the GA@GOx hybrid nanoflowers with compartmentalization had a better effect of the substrate channeling on the catalytic efficiency.
32156109	2	46	theme	robust	355:360	arg1	system					410:415	a robust and easy-to-use multienzymatic cascade reaction system	353:415	a robust and easy-to-use multienzymatic cascade reaction system of coimmobilized GA@GOx hybrid nanoflowers with a specific spatial distribution of enzymes by compartmentalization	353:530	In this study, a robust and easy-to-use multienzymatic cascade reaction system of coimmobilized GA@GOx hybrid nanoflowers with a specific spatial distribution of enzymes by compartmentalization was constructed and applied to catalyze starch to gluconic acid in one pot.
32156109	7	47	theme	conversion	1390:1399	arg1	efficiency					1401:1410	the conversion efficiency	1386:1410	the conversion efficiency	1386:1410	The final results indicated that the overall enzyme activity of the GA@GOx hybrid nanoflowers increased by 1.5 times, and the conversion efficiency was 92.12% within 80 min significantly superior to the free multienzyme system, which showed the outstanding conversion of starch into gluconic acid in one pot.
32156109	7	47	theme	conversion	1390:1399	arg1	%					1421:1421	92.12%	1416:1421	92.12%	1416:1421	The final results indicated that the overall enzyme activity of the GA@GOx hybrid nanoflowers increased by 1.5 times, and the conversion efficiency was 92.12% within 80 min significantly superior to the free multienzyme system, which showed the outstanding conversion of starch into gluconic acid in one pot.
32156109	1	48	theme	current	260:266	arg1	problems					268:275	the current problems	256:275	the current problems existing in traditional gluconic acid preparation processes	256:335	Introducing an efficient method for the rapid conversion of starch into gluconic acid is desirable to solve the current problems existing in traditional gluconic acid preparation processes.
32156109	1	49	theme	rapid	188:192	arg1	conversion					194:203	the rapid conversion	184:203	the rapid conversion of starch	184:213	Introducing an efficient method for the rapid conversion of starch into gluconic acid is desirable to solve the current problems existing in traditional gluconic acid preparation processes.
32156109	7	50	theme	hybrid	1339:1344	arg1	nanoflowers					1346:1356	the GA@GOx hybrid nanoflowers	1328:1356	the GA@GOx hybrid nanoflowers	1328:1356	The final results indicated that the overall enzyme activity of the GA@GOx hybrid nanoflowers increased by 1.5 times, and the conversion efficiency was 92.12% within 80 min significantly superior to the free multienzyme system, which showed the outstanding conversion of starch into gluconic acid in one pot.
32156109	0	51	theme	Gluconic	133:140	arg1	Acid					142:145	Gluconic Acid	133:145	Gluconic Acid	133:145	Construction of a Multienzymatic Cascade Reaction System of Coimmobilized Hybrid Nanoflowers for Efficient Conversion of Starch into Gluconic Acid.
32156109	7	52	theme	gluconic	1547:1554	arg1	acid					1556:1559	gluconic acid	1547:1559	gluconic acid in one pot	1547:1570	The final results indicated that the overall enzyme activity of the GA@GOx hybrid nanoflowers increased by 1.5 times, and the conversion efficiency was 92.12% within 80 min significantly superior to the free multienzyme system, which showed the outstanding conversion of starch into gluconic acid in one pot.
32156109	4	53	theme	preparation	886:896	arg1	conditions					898:907	The optimum preparation conditions	874:907	The optimum preparation conditions	874:907	The optimum preparation conditions and reaction parameters of the GA@GOx hybrid nanoflowers had been investigated.
32156109	7	54	theme	@	1334:1334	arg1	nanoflowers					1346:1356	the GA@GOx hybrid nanoflowers	1328:1356	the GA@GOx hybrid nanoflowers	1328:1356	The final results indicated that the overall enzyme activity of the GA@GOx hybrid nanoflowers increased by 1.5 times, and the conversion efficiency was 92.12% within 80 min significantly superior to the free multienzyme system, which showed the outstanding conversion of starch into gluconic acid in one pot.
32156109	7	55	theme	starch	1535:1540	arg1	conversion					1521:1530	the outstanding conversion	1505:1530	the outstanding conversion of starch into gluconic acid in one pot	1505:1570	The final results indicated that the overall enzyme activity of the GA@GOx hybrid nanoflowers increased by 1.5 times, and the conversion efficiency was 92.12% within 80 min significantly superior to the free multienzyme system, which showed the outstanding conversion of starch into gluconic acid in one pot.
32156109	6	56	theme	hybrid	1142:1147	arg1	nanoflowers					1149:1159	the GA@GOx hybrid nanoflowers	1131:1159	the GA@GOx hybrid nanoflowers with compartmentalization	1131:1185	Based on the lower Km, the GA@GOx hybrid nanoflowers with compartmentalization had a better effect of the substrate channeling on the catalytic efficiency.
32156109	2	57	with	nanoflowers	448:458	arg1	distribution					484:495	a specific spatial distribution	465:495	a specific spatial distribution of enzymes by compartmentalization	465:530	In this study, a robust and easy-to-use multienzymatic cascade reaction system of coimmobilized GA@GOx hybrid nanoflowers with a specific spatial distribution of enzymes by compartmentalization was constructed and applied to catalyze starch to gluconic acid in one pot.
32156109	3	58	theme	Cu2+	846:849	arg1	acids					861:865	amino acids	855:865	amino acids of GA	855:871	In the preparation processes, the glucose oxidase (GOx) hybrid nanoflowers were first synthesized via a self-assembly mechanism, and then, glucoamylase (GA) was adsorbed on the surface of GOx hybrid nanoflowers through the interaction of Cu2+ and amino acids of GA.
32156109	3	58	theme	Cu2+	846:849	arg1	interaction					831:841	the interaction	827:841	the interaction of Cu2+	827:849	In the preparation processes, the glucose oxidase (GOx) hybrid nanoflowers were first synthesized via a self-assembly mechanism, and then, glucoamylase (GA) was adsorbed on the surface of GOx hybrid nanoflowers through the interaction of Cu2+ and amino acids of GA.
32156109	6	59	with	nanoflowers	1149:1159	arg1	compartmentalization					1166:1185	compartmentalization	1166:1185	compartmentalization	1166:1185	Based on the lower Km, the GA@GOx hybrid nanoflowers with compartmentalization had a better effect of the substrate channeling on the catalytic efficiency.
32156109	2	60	theme	gluconic	582:589	arg1	acid					591:594	gluconic acid	582:594	gluconic acid in one pot	582:605	In this study, a robust and easy-to-use multienzymatic cascade reaction system of coimmobilized GA@GOx hybrid nanoflowers with a specific spatial distribution of enzymes by compartmentalization was constructed and applied to catalyze starch to gluconic acid in one pot.
32156109	7	61	theme	overall	1301:1307	arg1	activity					1316:1323	the overall enzyme activity	1297:1323	the overall enzyme activity of the GA@GOx hybrid nanoflowers	1297:1356	The final results indicated that the overall enzyme activity of the GA@GOx hybrid nanoflowers increased by 1.5 times, and the conversion efficiency was 92.12% within 80 min significantly superior to the free multienzyme system, which showed the outstanding conversion of starch into gluconic acid in one pot.
32156109	0	62	theme	Hybrid	74:79	arg1	Nanoflowers					81:91	Coimmobilized Hybrid Nanoflowers	60:91	Coimmobilized Hybrid Nanoflowers	60:91	Construction of a Multienzymatic Cascade Reaction System of Coimmobilized Hybrid Nanoflowers for Efficient Conversion of Starch into Gluconic Acid.
32156109	3	63	theme	preparation	615:625	arg1	processes					627:635	the preparation processes	611:635	the preparation processes	611:635	In the preparation processes, the glucose oxidase (GOx) hybrid nanoflowers were first synthesized via a self-assembly mechanism, and then, glucoamylase (GA) was adsorbed on the surface of GOx hybrid nanoflowers through the interaction of Cu2+ and amino acids of GA.
32156109	4	64	theme	@	942:942	arg1	nanoflowers					954:964	the GA@GOx hybrid nanoflowers	936:964	the GA@GOx hybrid nanoflowers	936:964	The optimum preparation conditions and reaction parameters of the GA@GOx hybrid nanoflowers had been investigated.
32156109	1	65	theme	efficient	163:171	arg1	method					173:178	an efficient method	160:178	an efficient method for the rapid conversion of starch	160:213	Introducing an efficient method for the rapid conversion of starch into gluconic acid is desirable to solve the current problems existing in traditional gluconic acid preparation processes.
32156109	4	66	theme	hybrid	947:952	arg1	nanoflowers					954:964	the GA@GOx hybrid nanoflowers	936:964	the GA@GOx hybrid nanoflowers	936:964	The optimum preparation conditions and reaction parameters of the GA@GOx hybrid nanoflowers had been investigated.
32156109	3	67	theme	amino	855:859	arg1	acids					861:865	amino acids	855:865	amino acids of GA	855:871	In the preparation processes, the glucose oxidase (GOx) hybrid nanoflowers were first synthesized via a self-assembly mechanism, and then, glucoamylase (GA) was adsorbed on the surface of GOx hybrid nanoflowers through the interaction of Cu2+ and amino acids of GA.
32156109	7	68	theme	free	1467:1470	arg1	system					1484:1489	the free multienzyme system	1463:1489	the free multienzyme system	1463:1489	The final results indicated that the overall enzyme activity of the GA@GOx hybrid nanoflowers increased by 1.5 times, and the conversion efficiency was 92.12% within 80 min significantly superior to the free multienzyme system, which showed the outstanding conversion of starch into gluconic acid in one pot.
32156109	3	69	theme	hybrid	800:805	arg1	nanoflowers					807:817	GOx hybrid nanoflowers	796:817	GOx hybrid nanoflowers	796:817	In the preparation processes, the glucose oxidase (GOx) hybrid nanoflowers were first synthesized via a self-assembly mechanism, and then, glucoamylase (GA) was adsorbed on the surface of GOx hybrid nanoflowers through the interaction of Cu2+ and amino acids of GA.
32156109	0	70	theme	Starch	121:126	arg1	Conversion					107:116	Efficient Conversion	97:116	Efficient Conversion of Starch into Gluconic Acid	97:145	Construction of a Multienzymatic Cascade Reaction System of Coimmobilized Hybrid Nanoflowers for Efficient Conversion of Starch into Gluconic Acid.
32156109	5	71	theme	@	1060:1060	arg1	nanoflowers					1072:1082	the GA@GOx hybrid nanoflowers	1054:1082	the GA@GOx hybrid nanoflowers	1054:1082	In addition, the morphology, composition, and crystallization of the GA@GOx hybrid nanoflowers had been fully studied.
32156109	7	72	theme	final	1268:1272	arg1	results					1274:1280	The final results	1264:1280	The final results	1264:1280	The final results indicated that the overall enzyme activity of the GA@GOx hybrid nanoflowers increased by 1.5 times, and the conversion efficiency was 92.12% within 80 min significantly superior to the free multienzyme system, which showed the outstanding conversion of starch into gluconic acid in one pot.
32156109	2	73	theme	reaction	401:408	arg1	system					410:415	a robust and easy-to-use multienzymatic cascade reaction system	353:415	a robust and easy-to-use multienzymatic cascade reaction system of coimmobilized GA@GOx hybrid nanoflowers with a specific spatial distribution of enzymes by compartmentalization	353:530	In this study, a robust and easy-to-use multienzymatic cascade reaction system of coimmobilized GA@GOx hybrid nanoflowers with a specific spatial distribution of enzymes by compartmentalization was constructed and applied to catalyze starch to gluconic acid in one pot.
32156109	5	74	theme	hybrid	1065:1070	arg1	nanoflowers					1072:1082	the GA@GOx hybrid nanoflowers	1054:1082	the GA@GOx hybrid nanoflowers	1054:1082	In addition, the morphology, composition, and crystallization of the GA@GOx hybrid nanoflowers had been fully studied.
32156109	2	75	theme	nanoflowers	448:458	arg1	system					410:415	a robust and easy-to-use multienzymatic cascade reaction system	353:415	a robust and easy-to-use multienzymatic cascade reaction system of coimmobilized GA@GOx hybrid nanoflowers with a specific spatial distribution of enzymes by compartmentalization	353:530	In this study, a robust and easy-to-use multienzymatic cascade reaction system of coimmobilized GA@GOx hybrid nanoflowers with a specific spatial distribution of enzymes by compartmentalization was constructed and applied to catalyze starch to gluconic acid in one pot.
32156109	2	76	theme	multienzymatic	378:391	arg1	system					410:415	a robust and easy-to-use multienzymatic cascade reaction system	353:415	a robust and easy-to-use multienzymatic cascade reaction system of coimmobilized GA@GOx hybrid nanoflowers with a specific spatial distribution of enzymes by compartmentalization	353:530	In this study, a robust and easy-to-use multienzymatic cascade reaction system of coimmobilized GA@GOx hybrid nanoflowers with a specific spatial distribution of enzymes by compartmentalization was constructed and applied to catalyze starch to gluconic acid in one pot.
32156109	0	77	theme	Multienzymatic	18:31	arg1	System					50:55	a Multienzymatic Cascade Reaction System	16:55	a Multienzymatic Cascade Reaction System of Coimmobilized Hybrid Nanoflowers for Efficient Conversion of Starch into Gluconic Acid	16:145	Construction of a Multienzymatic Cascade Reaction System of Coimmobilized Hybrid Nanoflowers for Efficient Conversion of Starch into Gluconic Acid.
32156109	2	78	theme	GOx	437:439	arg1	nanoflowers					448:458	coimmobilized GA@GOx hybrid nanoflowers	420:458	coimmobilized GA@GOx hybrid nanoflowers with a specific spatial distribution of enzymes by compartmentalization	420:530	In this study, a robust and easy-to-use multienzymatic cascade reaction system of coimmobilized GA@GOx hybrid nanoflowers with a specific spatial distribution of enzymes by compartmentalization was constructed and applied to catalyze starch to gluconic acid in one pot.
32156109	2	79	theme	GA	434:435	arg1	nanoflowers					448:458	coimmobilized GA@GOx hybrid nanoflowers	420:458	coimmobilized GA@GOx hybrid nanoflowers with a specific spatial distribution of enzymes by compartmentalization	420:530	In this study, a robust and easy-to-use multienzymatic cascade reaction system of coimmobilized GA@GOx hybrid nanoflowers with a specific spatial distribution of enzymes by compartmentalization was constructed and applied to catalyze starch to gluconic acid in one pot.
32156109	0	80	theme	Reaction	41:48	arg1	System					50:55	a Multienzymatic Cascade Reaction System	16:55	a Multienzymatic Cascade Reaction System of Coimmobilized Hybrid Nanoflowers for Efficient Conversion of Starch into Gluconic Acid	16:145	Construction of a Multienzymatic Cascade Reaction System of Coimmobilized Hybrid Nanoflowers for Efficient Conversion of Starch into Gluconic Acid.
32156109	1	81	theme	gluconic	301:308	arg1	acid					310:313	traditional gluconic acid	289:313	traditional gluconic acid preparation processes	289:335	Introducing an efficient method for the rapid conversion of starch into gluconic acid is desirable to solve the current problems existing in traditional gluconic acid preparation processes.
32156109	2	82	theme	spatial	476:482	arg1	distribution					484:495	a specific spatial distribution	465:495	a specific spatial distribution of enzymes by compartmentalization	465:530	In this study, a robust and easy-to-use multienzymatic cascade reaction system of coimmobilized GA@GOx hybrid nanoflowers with a specific spatial distribution of enzymes by compartmentalization was constructed and applied to catalyze starch to gluconic acid in one pot.
32156109	3	83	theme	glucose	642:648	arg1	GOx					659:661	GOx	659:661	GOx	659:661	In the preparation processes, the glucose oxidase (GOx) hybrid nanoflowers were first synthesized via a self-assembly mechanism, and then, glucoamylase (GA) was adsorbed on the surface of GOx hybrid nanoflowers through the interaction of Cu2+ and amino acids of GA.
32156109	3	83	theme	glucose	642:648	arg1	oxidase					650:656	glucose oxidase	642:656	the glucose oxidase (GOx) hybrid nanoflowers	638:681	In the preparation processes, the glucose oxidase (GOx) hybrid nanoflowers were first synthesized via a self-assembly mechanism, and then, glucoamylase (GA) was adsorbed on the surface of GOx hybrid nanoflowers through the interaction of Cu2+ and amino acids of GA.
32156109	1	84	theme	preparation	315:325	arg1	processes					327:335	traditional gluconic acid preparation processes	289:335	traditional gluconic acid preparation processes	289:335	Introducing an efficient method for the rapid conversion of starch into gluconic acid is desirable to solve the current problems existing in traditional gluconic acid preparation processes.
32156109	7	85	theme	superior	1451:1458	arg1	min					1433:1435	80 min	1430:1435	80 min significantly superior to the free multienzyme system, which showed the outstanding conversion of starch into gluconic acid in one pot	1430:1570	The final results indicated that the overall enzyme activity of the GA@GOx hybrid nanoflowers increased by 1.5 times, and the conversion efficiency was 92.12% within 80 min significantly superior to the free multienzyme system, which showed the outstanding conversion of starch into gluconic acid in one pot.
34293062	5	0	theme	phospholipase	627:639	arg1	A2					641:642	phospholipase A2	627:642	phospholipase A2	627:642	Chemical analyses of the bee venoms revealed average melittin, apamin, and phospholipase A2 contents of 40.57%, 2.12% and 13.67%, respectively.
34293062	6	1	theme	A2	766:767	arg1	content					769:775	a high phospholipase A2 content	745:775	a high phospholipase A2 content	745:775	The results suggest that Anatolian bee venom has a high phospholipase A2 content compared to the previous literature.
34293062	6	2	theme	high	747:750	arg1	A2					766:767	a high phospholipase A2	745:767	a high phospholipase A2 content	745:775	The results suggest that Anatolian bee venom has a high phospholipase A2 content compared to the previous literature.
34293062	12	3	theme	antimicrobial	1355:1367	arg1	character					1369:1377	its antimicrobial character	1351:1377	its antimicrobial character	1351:1377	A low microbial load was associated with a high phospholipase A2 content in the bee venom composition, thus contributing to its antimicrobial character.
34293062	8	4	theme	standard	937:944	arg1	values					946:951	standard values	937:951	standard values	937:951	Melittin content was within the range of standard values.
34293062	14	5	from	melittin	1559:1566	arg1	analysis					1615:1622	an analysis	1612:1622	an analysis of antimicrobial properties	1612:1650	The examination of other components in addition to phospholipase A2, melittin and apamin in future studies, together with an analysis of antimicrobial properties will further our understanding of Anatolian bee venom.
34293062	14	5	from	melittin	1559:1566	arg1	studies					1589:1595	future studies	1582:1595	future studies	1582:1595	The examination of other components in addition to phospholipase A2, melittin and apamin in future studies, together with an analysis of antimicrobial properties will further our understanding of Anatolian bee venom.
34293062	6	6	theme	bee	731:733	arg1	venom					735:739	Anatolian bee venom	721:739	Anatolian bee venom	721:739	The results suggest that Anatolian bee venom has a high phospholipase A2 content compared to the previous literature.
34293062	2	7	theme	microbiological	283:297	arg1	properties					299:308	microbiological properties	283:308	microbiological properties	283:308	The aim of this research paper is to determine the characteristics of Anatolian bee venom by evaluating its chemical content and microbiological properties.
34293062	14	8	from	apamin	1572:1577	arg1	analysis					1615:1622	an analysis	1612:1622	an analysis of antimicrobial properties	1612:1650	The examination of other components in addition to phospholipase A2, melittin and apamin in future studies, together with an analysis of antimicrobial properties will further our understanding of Anatolian bee venom.
34293062	14	8	from	apamin	1572:1577	arg1	studies					1589:1595	future studies	1582:1595	future studies	1582:1595	The examination of other components in addition to phospholipase A2, melittin and apamin in future studies, together with an analysis of antimicrobial properties will further our understanding of Anatolian bee venom.
34293062	12	9	theme	venom	1311:1315	arg1	composition					1317:1327	the bee venom composition	1303:1327	the bee venom composition	1303:1327	A low microbial load was associated with a high phospholipase A2 content in the bee venom composition, thus contributing to its antimicrobial character.
34293062	5	10	theme	%	668:668	arg1	contents					644:651	average melittin, apamin, and phospholipase A2 contents	597:651	average melittin, apamin, and phospholipase A2 contents of 40.57%, 2.12% and 13.67%, respectively	597:693	Chemical analyses of the bee venoms revealed average melittin, apamin, and phospholipase A2 contents of 40.57%, 2.12% and 13.67%, respectively.
34293062	14	11	theme	properties	1641:1650	arg1	analysis					1615:1622	an analysis	1612:1622	an analysis of antimicrobial properties	1612:1650	The examination of other components in addition to phospholipase A2, melittin and apamin in future studies, together with an analysis of antimicrobial properties will further our understanding of Anatolian bee venom.
34293062	12	12	theme	phospholipase	1275:1287	arg1	A2					1289:1290	a high phospholipase A2	1268:1290	a high phospholipase A2 content in the bee venom composition	1268:1327	A low microbial load was associated with a high phospholipase A2 content in the bee venom composition, thus contributing to its antimicrobial character.
34293062	13	13	theme	quality	1481:1487	arg1	terms					1441:1445	terms	1441:1445	terms of chemical content and microbial quality	1441:1487	This study presents an examination of Anatolian bee venom in terms of chemical content and microbial quality.
34293062	9	14	theme	Bee	954:956	arg1	samples					964:970	Bee venom samples	954:970	Bee venom samples	954:970	Bee venom samples were also observed to have a high sugar content, associated with pollen and nectar contamination.
34293062	12	15	from	content	1292:1298	arg1	composition					1317:1327	the bee venom composition	1303:1327	the bee venom composition	1303:1327	A low microbial load was associated with a high phospholipase A2 content in the bee venom composition, thus contributing to its antimicrobial character.
34293062	13	16	from	examination	1403:1413	arg1	terms					1441:1445	terms	1441:1445	terms of chemical content and microbial quality	1441:1487	This study presents an examination of Anatolian bee venom in terms of chemical content and microbial quality.
34293062	13	17	theme	chemical	1450:1457	arg1	content					1459:1465	chemical content	1450:1465	chemical content	1450:1465	This study presents an examination of Anatolian bee venom in terms of chemical content and microbial quality.
34293062	14	18	theme	other	1509:1513	arg1	components					1515:1524	other components	1509:1524	other components in addition to phospholipase A2, melittin and apamin in future studies, together with an analysis of antimicrobial properties	1509:1650	The examination of other components in addition to phospholipase A2, melittin and apamin in future studies, together with an analysis of antimicrobial properties will further our understanding of Anatolian bee venom.
34293062	10	19	theme	bee	1162:1164	arg1	venom					1166:1170	bee venom	1162:1170	bee venom	1162:1170	Total aerobic mesophilic bacteria counts revealed no microbial development in 11 samples of bee venom.
34293062	0	20	theme	contamination	58:70	arg1	evaluation					3:12	An evaluation	0:12	An evaluation of the chemical content and microbiological contamination of Anatolian bee venom.	0:94	An evaluation of the chemical content and microbiological contamination of Anatolian bee venom.
34293062	0	21	theme	Anatolian	75:83	arg1	venom					89:93	Anatolian bee venom	75:93	Anatolian bee venom	75:93	An evaluation of the chemical content and microbiological contamination of Anatolian bee venom.
34293062	5	22	theme	bee	577:579	arg1	venoms					581:586	the bee venoms	573:586	the bee venoms	573:586	Chemical analyses of the bee venoms revealed average melittin, apamin, and phospholipase A2 contents of 40.57%, 2.12% and 13.67%, respectively.
34293062	2	23	theme	bee	234:236	arg1	venom					238:242	Anatolian bee venom	224:242	Anatolian bee venom	224:242	The aim of this research paper is to determine the characteristics of Anatolian bee venom by evaluating its chemical content and microbiological properties.
34293062	0	24	theme	venom	89:93	arg1	content					30:36	chemical content	21:36	chemical content	21:36	An evaluation of the chemical content and microbiological contamination of Anatolian bee venom.
34293062	0	24	theme	venom	89:93	arg1	contamination					58:70	microbiological contamination	42:70	microbiological contamination	42:70	An evaluation of the chemical content and microbiological contamination of Anatolian bee venom.
34293062	9	25	theme	sugar	1006:1010	arg1	content					1012:1018	a high sugar content	999:1018	a high sugar content	999:1018	Bee venom samples were also observed to have a high sugar content, associated with pollen and nectar contamination.
34293062	11	26	located	detected	1203:1210	arg1	sample					1219:1224	any sample	1215:1224	any sample	1215:1224	Staphylococcus aureus was not detected in any sample.
34293062	11	26	located	detected	1203:1210	arg2	aureus					1188:1193	Staphylococcus aureus	1173:1193	Staphylococcus aureus	1173:1193	Staphylococcus aureus was not detected in any sample.
34293062	5	27	theme	Chemical	552:559	arg1	analyses					561:568	Chemical analyses	552:568	Chemical analyses of the bee venoms	552:586	Chemical analyses of the bee venoms revealed average melittin, apamin, and phospholipase A2 contents of 40.57%, 2.12% and 13.67%, respectively.
34293062	10	28	theme	Total	1070:1074	arg1	counts					1104:1109	Total aerobic mesophilic bacteria counts	1070:1109	Total aerobic mesophilic bacteria counts	1070:1109	Total aerobic mesophilic bacteria counts revealed no microbial development in 11 samples of bee venom.
34293062	12	29	theme	microbial	1233:1241	arg1	load					1243:1246	A low microbial load	1227:1246	A low microbial load	1227:1246	A low microbial load was associated with a high phospholipase A2 content in the bee venom composition, thus contributing to its antimicrobial character.
34293062	2	30	theme	Anatolian	224:232	arg1	venom					238:242	Anatolian bee venom	224:242	Anatolian bee venom	224:242	The aim of this research paper is to determine the characteristics of Anatolian bee venom by evaluating its chemical content and microbiological properties.
34293062	3	31	theme	Turkey	432:437	arg1	areas					413:417	different areas	403:417	different areas of Anatolia, Turkey	403:437	Physical, chemical and microbiological analyses were performed on 25 bee venom samples from different areas of Anatolia, Turkey.
34293062	0	32	theme	chemical	21:28	arg1	content					30:36	chemical content	21:36	chemical content	21:36	An evaluation of the chemical content and microbiological contamination of Anatolian bee venom.
34293062	3	33	theme	venom	384:388	arg1	samples					390:396	25 bee venom samples	377:396	25 bee venom samples from different areas of Anatolia, Turkey	377:437	Physical, chemical and microbiological analyses were performed on 25 bee venom samples from different areas of Anatolia, Turkey.
34293062	4	34	theme	one-way	511:517	arg1	ANOVA					531:535	one-way and two-way ANOVA	511:535	ANOVA	531:535	Data obtained by 3-replicate studies were evaluated with normality and one-way and two-way ANOVA / Tukey tests.
34293062	10	35	theme	mesophilic	1084:1093	arg1	bacteria					1095:1102	aerobic mesophilic bacteria	1076:1102	Total aerobic mesophilic bacteria counts	1070:1109	Total aerobic mesophilic bacteria counts revealed no microbial development in 11 samples of bee venom.
34293062	4	36	theme	two-way	523:529	arg1	ANOVA					531:535	one-way and two-way ANOVA	511:535	ANOVA	531:535	Data obtained by 3-replicate studies were evaluated with normality and one-way and two-way ANOVA / Tukey tests.
34293062	2	37	theme	paper	179:183	arg1	aim					158:160	The aim	154:160	The aim of this research paper	154:183	The aim of this research paper is to determine the characteristics of Anatolian bee venom by evaluating its chemical content and microbiological properties.
34293062	14	38	theme	venom	1700:1704	arg1	understanding					1669:1681	our understanding	1665:1681	our understanding of Anatolian bee venom	1665:1704	The examination of other components in addition to phospholipase A2, melittin and apamin in future studies, together with an analysis of antimicrobial properties will further our understanding of Anatolian bee venom.
34293062	8	39	theme	Melittin	896:903	arg1	content					905:911	Melittin content	896:911	Melittin content	896:911	Melittin content was within the range of standard values.
34293062	13	40	theme	Anatolian	1418:1426	arg1	venom					1432:1436	Anatolian bee venom	1418:1436	Anatolian bee venom	1418:1436	This study presents an examination of Anatolian bee venom in terms of chemical content and microbial quality.
34293062	14	41	theme	Anatolian	1686:1694	arg1	venom					1700:1704	Anatolian bee venom	1686:1704	Anatolian bee venom	1686:1704	The examination of other components in addition to phospholipase A2, melittin and apamin in future studies, together with an analysis of antimicrobial properties will further our understanding of Anatolian bee venom.
34293062	5	42	theme	A2	641:642	arg1	contents					644:651	average melittin, apamin, and phospholipase A2 contents	597:651	average melittin, apamin, and phospholipase A2 contents of 40.57%, 2.12% and 13.67%, respectively	597:693	Chemical analyses of the bee venoms revealed average melittin, apamin, and phospholipase A2 contents of 40.57%, 2.12% and 13.67%, respectively.
34293062	6	43	theme	phospholipase	752:764	arg1	A2					766:767	a high phospholipase A2	745:767	a high phospholipase A2 content	745:775	The results suggest that Anatolian bee venom has a high phospholipase A2 content compared to the previous literature.
34293062	2	44	theme	chemical	262:269	arg1	content					271:277	its chemical content	258:277	its chemical content	258:277	The aim of this research paper is to determine the characteristics of Anatolian bee venom by evaluating its chemical content and microbiological properties.
34293062	5	45	theme	melittin	605:612	arg1	contents					644:651	average melittin, apamin, and phospholipase A2 contents	597:651	average melittin, apamin, and phospholipase A2 contents of 40.57%, 2.12% and 13.67%, respectively	597:693	Chemical analyses of the bee venoms revealed average melittin, apamin, and phospholipase A2 contents of 40.57%, 2.12% and 13.67%, respectively.
34293062	8	46	theme	values	946:951	arg1	range					928:932	the range	924:932	the range of standard values	924:951	Melittin content was within the range of standard values.
34293062	5	47	theme	apamin	615:620	arg1	contents					644:651	average melittin, apamin, and phospholipase A2 contents	597:651	average melittin, apamin, and phospholipase A2 contents of 40.57%, 2.12% and 13.67%, respectively	597:693	Chemical analyses of the bee venoms revealed average melittin, apamin, and phospholipase A2 contents of 40.57%, 2.12% and 13.67%, respectively.
34293062	6	48	theme	Anatolian	721:729	arg1	venom					735:739	Anatolian bee venom	721:739	Anatolian bee venom	721:739	The results suggest that Anatolian bee venom has a high phospholipase A2 content compared to the previous literature.
34293062	12	49	theme	bee	1307:1309	arg1	composition					1317:1327	the bee venom composition	1303:1327	the bee venom composition	1303:1327	A low microbial load was associated with a high phospholipase A2 content in the bee venom composition, thus contributing to its antimicrobial character.
34293062	10	50	from	development	1133:1143	arg1	samples					1151:1157	11 samples	1148:1157	11 samples of bee venom	1148:1170	Total aerobic mesophilic bacteria counts revealed no microbial development in 11 samples of bee venom.
34293062	14	51	theme	antimicrobial	1627:1639	arg1	properties					1641:1650	antimicrobial properties	1627:1650	antimicrobial properties	1627:1650	The examination of other components in addition to phospholipase A2, melittin and apamin in future studies, together with an analysis of antimicrobial properties will further our understanding of Anatolian bee venom.
34293062	13	52	theme	microbial	1471:1479	arg1	quality					1481:1487	microbial quality	1471:1487	microbial quality	1471:1487	This study presents an examination of Anatolian bee venom in terms of chemical content and microbial quality.
34293062	12	53	theme	high	1270:1273	arg1	A2					1289:1290	a high phospholipase A2	1268:1290	a high phospholipase A2 content in the bee venom composition	1268:1327	A low microbial load was associated with a high phospholipase A2 content in the bee venom composition, thus contributing to its antimicrobial character.
34293062	14	54	theme	phospholipase	1541:1553	arg1	melittin					1559:1566	melittin	1559:1566	melittin	1559:1566	The examination of other components in addition to phospholipase A2, melittin and apamin in future studies, together with an analysis of antimicrobial properties will further our understanding of Anatolian bee venom.
34293062	14	54	theme	phospholipase	1541:1553	arg1	A2					1555:1556	phospholipase A2	1541:1556	phospholipase A2	1541:1556	The examination of other components in addition to phospholipase A2, melittin and apamin in future studies, together with an analysis of antimicrobial properties will further our understanding of Anatolian bee venom.
34293062	14	54	theme	phospholipase	1541:1553	arg1	apamin					1572:1577	apamin	1572:1577	apamin	1572:1577	The examination of other components in addition to phospholipase A2, melittin and apamin in future studies, together with an analysis of antimicrobial properties will further our understanding of Anatolian bee venom.
34293062	12	55	theme	A2	1289:1290	arg1	content					1292:1298	a high phospholipase A2 content	1268:1298	a high phospholipase A2 content in the bee venom composition	1268:1327	A low microbial load was associated with a high phospholipase A2 content in the bee venom composition, thus contributing to its antimicrobial character.
34293062	9	56	theme	venom	958:962	arg1	samples					964:970	Bee venom samples	954:970	Bee venom samples	954:970	Bee venom samples were also observed to have a high sugar content, associated with pollen and nectar contamination.
34293062	5	57	theme	%	661:661	arg1	contents					644:651	average melittin, apamin, and phospholipase A2 contents	597:651	average melittin, apamin, and phospholipase A2 contents of 40.57%, 2.12% and 13.67%, respectively	597:693	Chemical analyses of the bee venoms revealed average melittin, apamin, and phospholipase A2 contents of 40.57%, 2.12% and 13.67%, respectively.
34293062	9	58	contain	have	994:997	arg1	samples					964:970	Bee venom samples	954:970	Bee venom samples	954:970	Bee venom samples were also observed to have a high sugar content, associated with pollen and nectar contamination.
34293062	9	58	contain	have	994:997	arg2	content					1012:1018	a high sugar content	999:1018	a high sugar content	999:1018	Bee venom samples were also observed to have a high sugar content, associated with pollen and nectar contamination.
34293062	2	59	theme	venom	238:242	arg1	characteristics					205:219	the characteristics	201:219	the characteristics of Anatolian bee venom	201:242	The aim of this research paper is to determine the characteristics of Anatolian bee venom by evaluating its chemical content and microbiological properties.
34293062	1	60	theme	natural	111:117	arg1	substance					119:127	a natural substance	109:127	a natural substance produced by worker bees	109:151	Bee venom is a natural substance produced by worker bees.
34293062	1	60	theme	natural	111:117	arg1	venom					100:104	Bee venom	96:104	Bee venom	96:104	Bee venom is a natural substance produced by worker bees.
34293062	14	61	theme	components	1515:1524	arg1	examination					1494:1504	The examination	1490:1504	The examination of other components in addition to phospholipase A2, melittin and apamin in future studies, together with an analysis of antimicrobial properties	1490:1650	The examination of other components in addition to phospholipase A2, melittin and apamin in future studies, together with an analysis of antimicrobial properties will further our understanding of Anatolian bee venom.
34293062	10	62	theme	venom	1166:1170	arg1	samples					1151:1157	11 samples	1148:1157	11 samples of bee venom	1148:1170	Total aerobic mesophilic bacteria counts revealed no microbial development in 11 samples of bee venom.
34293062	13	63	theme	content	1459:1465	arg1	terms					1441:1445	terms	1441:1445	terms of chemical content and microbial quality	1441:1487	This study presents an examination of Anatolian bee venom in terms of chemical content and microbial quality.
34293062	3	64	theme	chemical	321:328	arg1	analyses					350:357	Physical, chemical and microbiological analyses	311:357	Physical, chemical and microbiological analyses	311:357	Physical, chemical and microbiological analyses were performed on 25 bee venom samples from different areas of Anatolia, Turkey.
34293062	0	65	theme	bee	85:87	arg1	venom					89:93	Anatolian bee venom	75:93	Anatolian bee venom	75:93	An evaluation of the chemical content and microbiological contamination of Anatolian bee venom.
34293062	14	66	theme	future	1582:1587	arg1	studies					1589:1595	future studies	1582:1595	future studies	1582:1595	The examination of other components in addition to phospholipase A2, melittin and apamin in future studies, together with an analysis of antimicrobial properties will further our understanding of Anatolian bee venom.
34293062	1	67	theme	worker	141:146	arg1	bees					148:151	worker bees	141:151	worker bees	141:151	Bee venom is a natural substance produced by worker bees.
34293062	3	68	theme	Physical	311:318	arg1	analyses					350:357	Physical, chemical and microbiological analyses	311:357	Physical, chemical and microbiological analyses	311:357	Physical, chemical and microbiological analyses were performed on 25 bee venom samples from different areas of Anatolia, Turkey.
34293062	5	69	theme	%	679:679	arg1	contents					644:651	average melittin, apamin, and phospholipase A2 contents	597:651	average melittin, apamin, and phospholipase A2 contents of 40.57%, 2.12% and 13.67%, respectively	597:693	Chemical analyses of the bee venoms revealed average melittin, apamin, and phospholipase A2 contents of 40.57%, 2.12% and 13.67%, respectively.
34293062	5	70	theme	venoms	581:586	arg1	analyses					561:568	Chemical analyses	552:568	Chemical analyses of the bee venoms	552:586	Chemical analyses of the bee venoms revealed average melittin, apamin, and phospholipase A2 contents of 40.57%, 2.12% and 13.67%, respectively.
34293062	9	71	dep	pollen	1037:1042	arg1	contamination					1055:1067	contamination	1055:1067	contamination	1055:1067	Bee venom samples were also observed to have a high sugar content, associated with pollen and nectar contamination.
34293062	4	72	theme	3-replicate	457:467	arg1	studies					469:475	3-replicate studies	457:475	3-replicate studies	457:475	Data obtained by 3-replicate studies were evaluated with normality and one-way and two-way ANOVA / Tukey tests.
34293062	9	73	theme	high	1001:1004	arg1	content					1012:1018	a high sugar content	999:1018	a high sugar content	999:1018	Bee venom samples were also observed to have a high sugar content, associated with pollen and nectar contamination.
34293062	5	74	theme	average	597:603	arg1	contents					644:651	average melittin, apamin, and phospholipase A2 contents	597:651	average melittin, apamin, and phospholipase A2 contents of 40.57%, 2.12% and 13.67%, respectively	597:693	Chemical analyses of the bee venoms revealed average melittin, apamin, and phospholipase A2 contents of 40.57%, 2.12% and 13.67%, respectively.
34293062	12	75	theme	low	1229:1231	arg1	load					1243:1246	A low microbial load	1227:1246	A low microbial load	1227:1246	A low microbial load was associated with a high phospholipase A2 content in the bee venom composition, thus contributing to its antimicrobial character.
34293062	3	76	theme	microbiological	334:348	arg1	analyses					350:357	Physical, chemical and microbiological analyses	311:357	Physical, chemical and microbiological analyses	311:357	Physical, chemical and microbiological analyses were performed on 25 bee venom samples from different areas of Anatolia, Turkey.
34293062	14	77	theme	bee	1696:1698	arg1	venom					1700:1704	Anatolian bee venom	1686:1704	Anatolian bee venom	1686:1704	The examination of other components in addition to phospholipase A2, melittin and apamin in future studies, together with an analysis of antimicrobial properties will further our understanding of Anatolian bee venom.
34293062	13	78	theme	venom	1432:1436	arg1	examination					1403:1413	an examination	1400:1413	an examination of Anatolian bee venom in terms of chemical content and microbial quality	1400:1487	This study presents an examination of Anatolian bee venom in terms of chemical content and microbial quality.
34293062	3	79	theme	bee	380:382	arg1	samples					390:396	25 bee venom samples	377:396	25 bee venom samples from different areas of Anatolia, Turkey	377:437	Physical, chemical and microbiological analyses were performed on 25 bee venom samples from different areas of Anatolia, Turkey.
34293062	4	80	theme	Tukey	539:543	arg1	tests					545:549	one-way and two-way ANOVA / Tukey tests	511:549	one-way and two-way ANOVA / Tukey tests	511:549	Data obtained by 3-replicate studies were evaluated with normality and one-way and two-way ANOVA / Tukey tests.
34293062	1	81	theme	Bee	96:98	arg1	substance					119:127	a natural substance	109:127	a natural substance produced by worker bees	109:151	Bee venom is a natural substance produced by worker bees.
34293062	1	81	theme	Bee	96:98	arg1	venom					100:104	Bee venom	96:104	Bee venom	96:104	Bee venom is a natural substance produced by worker bees.
34293062	14	82	dep	together	1598:1605	arg1	with					1607:1610	with	1607:1610	with	1607:1610	The examination of other components in addition to phospholipase A2, melittin and apamin in future studies, together with an analysis of antimicrobial properties will further our understanding of Anatolian bee venom.
34293062	0	83	theme	content	30:36	arg1	evaluation					3:12	An evaluation	0:12	An evaluation of the chemical content and microbiological contamination of Anatolian bee venom.	0:94	An evaluation of the chemical content and microbiological contamination of Anatolian bee venom.
34293062	3	84	theme	different	403:411	arg1	areas					413:417	different areas	403:417	different areas of Anatolia, Turkey	403:437	Physical, chemical and microbiological analyses were performed on 25 bee venom samples from different areas of Anatolia, Turkey.
34293062	6	85	contain	has	741:743	arg1	venom					735:739	Anatolian bee venom	721:739	Anatolian bee venom	721:739	The results suggest that Anatolian bee venom has a high phospholipase A2 content compared to the previous literature.
34293062	6	85	contain	has	741:743	arg2	content					769:775	a high phospholipase A2 content	745:775	a high phospholipase A2 content	745:775	The results suggest that Anatolian bee venom has a high phospholipase A2 content compared to the previous literature.
34293062	0	86	theme	microbiological	42:56	arg1	contamination					58:70	microbiological contamination	42:70	microbiological contamination	42:70	An evaluation of the chemical content and microbiological contamination of Anatolian bee venom.
34293062	10	87	theme	bacteria	1095:1102	arg1	counts					1104:1109	Total aerobic mesophilic bacteria counts	1070:1109	Total aerobic mesophilic bacteria counts	1070:1109	Total aerobic mesophilic bacteria counts revealed no microbial development in 11 samples of bee venom.
34293062	6	88	theme	previous	793:800	arg1	literature					802:811	the previous literature	789:811	the previous literature	789:811	The results suggest that Anatolian bee venom has a high phospholipase A2 content compared to the previous literature.
34293062	0	89	dep	content	30:36	arg1	the					17:19	the	17:19	the	17:19	An evaluation of the chemical content and microbiological contamination of Anatolian bee venom.
34293062	4	90	theme	ANOVA	531:535	arg1	tests					545:549	one-way and two-way ANOVA / Tukey tests	511:549	one-way and two-way ANOVA / Tukey tests	511:549	Data obtained by 3-replicate studies were evaluated with normality and one-way and two-way ANOVA / Tukey tests.
34293062	10	91	theme	aerobic	1076:1082	arg1	bacteria					1095:1102	aerobic mesophilic bacteria	1076:1102	Total aerobic mesophilic bacteria counts	1070:1109	Total aerobic mesophilic bacteria counts revealed no microbial development in 11 samples of bee venom.
34293062	7	92	theme	other	879:883	arg1	countries					885:893	other countries	879:893	other countries	879:893	The results for apamin content were similar to those reported in other countries.
34293062	2	93	theme	research	170:177	arg1	paper					179:183	this research paper	165:183	this research paper	165:183	The aim of this research paper is to determine the characteristics of Anatolian bee venom by evaluating its chemical content and microbiological properties.
34293062	3	94	from	areas	413:417	arg1	samples					390:396	25 bee venom samples	377:396	25 bee venom samples from different areas of Anatolia, Turkey	377:437	Physical, chemical and microbiological analyses were performed on 25 bee venom samples from different areas of Anatolia, Turkey.
34293062	10	95	theme	microbial	1123:1131	arg1	development					1133:1143	no microbial development	1120:1143	no microbial development in 11 samples of bee venom	1120:1170	Total aerobic mesophilic bacteria counts revealed no microbial development in 11 samples of bee venom.
34293062	13	96	theme	bee	1428:1430	arg1	venom					1432:1436	Anatolian bee venom	1418:1436	Anatolian bee venom	1418:1436	This study presents an examination of Anatolian bee venom in terms of chemical content and microbial quality.
34293062	7	97	theme	apamin	830:835	arg1	content					837:843	apamin content	830:843	apamin content	830:843	The results for apamin content were similar to those reported in other countries.
33803805	6	0	theme	mesenchymal	897:907	arg1	hMSCs					921:925	hMSCs	921:925	hMSCs	921:925	In this study, bone marrow-derived human mesenchymal stem cells (hMSCs) were exposed to fluid shear stress of 10 Pa.
33803805	6	0	theme	mesenchymal	897:907	arg1	cells					914:918	bone marrow-derived human mesenchymal stem cells	871:918	bone marrow-derived human mesenchymal stem cells (hMSCs)	871:926	In this study, bone marrow-derived human mesenchymal stem cells (hMSCs) were exposed to fluid shear stress of 10 Pa.
33803805	9	1	with	treatment	1183:1191	arg1	stress					1204:1209	shear stress	1198:1209	shear stress using confocal microscopy	1198:1235	The cellular structure of hMSCs and HAS2-overexpressing hMSCs was investigated after treatment with shear stress using confocal microscopy.
33803805	9	2	theme	hMSCs	1124:1128	arg1	structure					1111:1119	The cellular structure	1098:1119	The cellular structure of hMSCs and HAS2-overexpressing hMSCs	1098:1158	The cellular structure of hMSCs and HAS2-overexpressing hMSCs was investigated after treatment with shear stress using confocal microscopy.
33803805	3	3	theme	Hyaluronan	504:513	arg1	synthases					515:523	Hyaluronan synthases	504:523	Hyaluronan synthases (HAS)	504:529	Hyaluronan synthases (HAS) belong to the same enzyme family as chitin synthases.
33803805	10	4	theme	Fluid	1238:1242	arg1	stress					1250:1255	Fluid shear stress	1238:1255	Fluid shear stress	1238:1255	Fluid shear stress upregulated the expression of genes that encode proteins belonging to the HA biosynthesis and bone mineralization pathways.
33803805	0	5	theme	Shear	67:71	arg1	Stress					73:78	Fluid Shear Stress	61:78	Fluid Shear Stress in Bone Marrow-Derived Mesenchymal Stem Cells	61:124	Hyaluronan Synthases' Expression and Activity Are Induced by Fluid Shear Stress in Bone Marrow-Derived Mesenchymal Stem Cells.
33803805	6	6	theme	human	891:895	arg1	hMSCs					921:925	hMSCs	921:925	hMSCs	921:925	In this study, bone marrow-derived human mesenchymal stem cells (hMSCs) were exposed to fluid shear stress of 10 Pa.
33803805	6	6	theme	human	891:895	arg1	cells					914:918	bone marrow-derived human mesenchymal stem cells	871:918	bone marrow-derived human mesenchymal stem cells (hMSCs)	871:926	In this study, bone marrow-derived human mesenchymal stem cells (hMSCs) were exposed to fluid shear stress of 10 Pa.
33803805	12	7	theme	enzymatic	1502:1510	arg1	activation					1512:1521	enzymatic activation	1502:1521	enzymatic activation	1502:1521	Knowledge about the regulation mechanism governing HAS expression, trafficking, enzymatic activation and quality of the HA product in hMSCs is essential to understand the biological role of HA in the bone microenvironment.
33803805	9	8	theme	shear	1198:1202	arg1	stress					1204:1209	shear stress	1198:1209	shear stress using confocal microscopy	1198:1235	The cellular structure of hMSCs and HAS2-overexpressing hMSCs was investigated after treatment with shear stress using confocal microscopy.
33803805	4	9	contain	have	653:656	arg1	hyaluronan					599:608	Their product hyaluronan	585:608	Their product hyaluronan (HA)	585:613	Their product hyaluronan (HA) occurs in the bone and is supposed to have a regulatory function during bone regeneration.
33803805	4	9	contain	have	653:656	arg2	function					671:678	a regulatory function	658:678	a regulatory function	658:678	Their product hyaluronan (HA) occurs in the bone and is supposed to have a regulatory function during bone regeneration.
33803805	4	9	contain	have	653:656	arg1	HA					611:612	HA	611:612	HA	611:612	Their product hyaluronan (HA) occurs in the bone and is supposed to have a regulatory function during bone regeneration.
33803805	12	10	theme	HA	1542:1543	arg1	product					1545:1551	the HA product	1538:1551	the HA product	1538:1551	Knowledge about the regulation mechanism governing HAS expression, trafficking, enzymatic activation and quality of the HA product in hMSCs is essential to understand the biological role of HA in the bone microenvironment.
33803805	9	11	theme	HAS2-overexpressing	1134:1152	arg1	hMSCs					1154:1158	HAS2-overexpressing hMSCs	1134:1158	HAS2-overexpressing hMSCs	1134:1158	The cellular structure of hMSCs and HAS2-overexpressing hMSCs was investigated after treatment with shear stress using confocal microscopy.
33803805	10	12	theme	HA	1331:1332	arg1	biosynthesis					1334:1345	HA biosynthesis	1331:1345	HA biosynthesis	1331:1345	Fluid shear stress upregulated the expression of genes that encode proteins belonging to the HA biosynthesis and bone mineralization pathways.
33803805	10	13	theme	bone	1351:1354	arg1	mineralization					1356:1369	bone mineralization	1351:1369	bone mineralization	1351:1369	Fluid shear stress upregulated the expression of genes that encode proteins belonging to the HA biosynthesis and bone mineralization pathways.
33803805	6	14	theme	bone	871:874	arg1	hMSCs					921:925	hMSCs	921:925	hMSCs	921:925	In this study, bone marrow-derived human mesenchymal stem cells (hMSCs) were exposed to fluid shear stress of 10 Pa.
33803805	6	14	theme	bone	871:874	arg1	cells					914:918	bone marrow-derived human mesenchymal stem cells	871:918	bone marrow-derived human mesenchymal stem cells (hMSCs)	871:926	In this study, bone marrow-derived human mesenchymal stem cells (hMSCs) were exposed to fluid shear stress of 10 Pa.
33803805	6	15	theme	shear	950:954	arg1	stress					956:961	fluid shear stress	944:961	fluid shear stress of 10 Pa.	944:971	In this study, bone marrow-derived human mesenchymal stem cells (hMSCs) were exposed to fluid shear stress of 10 Pa.
33803805	9	16	theme	hMSCs	1154:1158	arg1	structure					1111:1119	The cellular structure	1098:1119	The cellular structure of hMSCs and HAS2-overexpressing hMSCs	1098:1158	The cellular structure of hMSCs and HAS2-overexpressing hMSCs was investigated after treatment with shear stress using confocal microscopy.
33803805	4	17	theme	regulatory	660:669	arg1	function					671:678	a regulatory function	658:678	a regulatory function	658:678	Their product hyaluronan (HA) occurs in the bone and is supposed to have a regulatory function during bone regeneration.
33803805	1	18	theme	growing	257:263	arg1	tissue					277:282	the growing mineralized tissue	253:282	the growing mineralized tissue	253:282	During biomineralization, the cells generating the biominerals must be able to sense the external physical stimuli exerted by the growing mineralized tissue and change their intracellular protein composition according to these stimuli.
33803805	0	19	theme	Mesenchymal	103:113	arg1	Cells					120:124	Bone Marrow-Derived Mesenchymal Stem Cells	83:124	Bone Marrow-Derived Mesenchymal Stem Cells	83:124	Hyaluronan Synthases' Expression and Activity Are Induced by Fluid Shear Stress in Bone Marrow-Derived Mesenchymal Stem Cells.
33803805	10	20	theme	mineralization	1356:1369	arg1	pathways					1371:1378	the HA biosynthesis and bone mineralization pathways	1327:1378	the HA biosynthesis and bone mineralization pathways	1327:1378	Fluid shear stress upregulated the expression of genes that encode proteins belonging to the HA biosynthesis and bone mineralization pathways.
33803805	5	21	theme	chitin	838:843	arg1	synthases					845:853	molluscan chitin synthases	828:853	molluscan chitin synthases	828:853	We hypothesize that HASes' expression and activity are controlled by fluid-induced mechanotransduction as it is known for molluscan chitin synthases.
33803805	0	22	theme	Marrow-Derived	88:101	arg1	Cells					120:124	Bone Marrow-Derived Mesenchymal Stem Cells	83:124	Bone Marrow-Derived Mesenchymal Stem Cells	83:124	Hyaluronan Synthases' Expression and Activity Are Induced by Fluid Shear Stress in Bone Marrow-Derived Mesenchymal Stem Cells.
33803805	4	23	theme	product	591:597	arg1	hyaluronan					599:608	Their product hyaluronan	585:608	Their product hyaluronan (HA)	585:613	Their product hyaluronan (HA) occurs in the bone and is supposed to have a regulatory function during bone regeneration.
33803805	4	23	theme	product	591:597	arg1	HA					611:612	HA	611:612	HA	611:612	Their product hyaluronan (HA) occurs in the bone and is supposed to have a regulatory function during bone regeneration.
33803805	2	24	theme	myosin-chitin	387:399	arg1	link					441:444	the link	437:444	the link for this communication between cells and the biomaterial	437:501	In molluscan shell, the myosin-chitin synthases have been suggested to be the link for this communication between cells and the biomaterial.
33803805	2	24	theme	myosin-chitin	387:399	arg1	synthases					401:409	the myosin-chitin synthases	383:409	the myosin-chitin synthases	383:409	In molluscan shell, the myosin-chitin synthases have been suggested to be the link for this communication between cells and the biomaterial.
33803805	5	25	theme	fluid-induced	775:787	arg1	mechanotransduction					789:807	fluid-induced mechanotransduction	775:807	fluid-induced mechanotransduction	775:807	We hypothesize that HASes' expression and activity are controlled by fluid-induced mechanotransduction as it is known for molluscan chitin synthases.
33803805	6	26	theme	fluid	944:948	arg1	stress					956:961	fluid shear stress	944:961	fluid shear stress of 10 Pa.	944:971	In this study, bone marrow-derived human mesenchymal stem cells (hMSCs) were exposed to fluid shear stress of 10 Pa.
33803805	0	27	theme	Hyaluronan	0:9	arg1	Activity					37:44	Activity	37:44	Activity	37:44	Hyaluronan Synthases' Expression and Activity Are Induced by Fluid Shear Stress in Bone Marrow-Derived Mesenchymal Stem Cells.
33803805	0	27	theme	Hyaluronan	0:9	arg1	Expression					22:31	Expression	22:31	Expression	22:31	Hyaluronan Synthases' Expression and Activity Are Induced by Fluid Shear Stress in Bone Marrow-Derived Mesenchymal Stem Cells.
33803805	0	27	theme	Hyaluronan	0:9	arg1	Synthases					11:19	Hyaluronan Synthases	0:19	Hyaluronan Synthases' Expression and Activity	0:44	Hyaluronan Synthases' Expression and Activity Are Induced by Fluid Shear Stress in Bone Marrow-Derived Mesenchymal Stem Cells.
33803805	1	28	theme	mineralized	265:275	arg1	tissue					277:282	the growing mineralized tissue	253:282	the growing mineralized tissue	253:282	During biomineralization, the cells generating the biominerals must be able to sense the external physical stimuli exerted by the growing mineralized tissue and change their intracellular protein composition according to these stimuli.
33803805	3	29	dep	synthases	515:523	arg1	HAS					526:528	HAS	526:528	HAS	526:528	Hyaluronan synthases (HAS) belong to the same enzyme family as chitin synthases.
33803805	11	30	theme	HAS	1385:1387	arg1	activity					1389:1396	The HAS activity	1381:1396	The HAS activity	1381:1396	The HAS activity appeared to be induced.
33803805	8	31	theme	HA	1036:1037	arg1	concentrations					1039:1052	HA concentrations	1036:1052	HA concentrations in the supernatants	1036:1072	HA concentrations in the supernatants were measured by ELISA.
33803805	3	32	theme	same	545:548	arg1	family					557:562	the same enzyme family	541:562	the same enzyme family as chitin synthases	541:582	Hyaluronan synthases (HAS) belong to the same enzyme family as chitin synthases.
33803805	12	33	theme	HA	1612:1613	arg1	role					1604:1607	the biological role	1589:1607	the biological role of HA in the bone microenvironment	1589:1642	Knowledge about the regulation mechanism governing HAS expression, trafficking, enzymatic activation and quality of the HA product in hMSCs is essential to understand the biological role of HA in the bone microenvironment.
33803805	0	34	theme	Stem	115:118	arg1	Cells					120:124	Bone Marrow-Derived Mesenchymal Stem Cells	83:124	Bone Marrow-Derived Mesenchymal Stem Cells	83:124	Hyaluronan Synthases' Expression and Activity Are Induced by Fluid Shear Stress in Bone Marrow-Derived Mesenchymal Stem Cells.
33803805	0	35	from	Stress	73:78	arg1	Cells					120:124	Bone Marrow-Derived Mesenchymal Stem Cells	83:124	Bone Marrow-Derived Mesenchymal Stem Cells	83:124	Hyaluronan Synthases' Expression and Activity Are Induced by Fluid Shear Stress in Bone Marrow-Derived Mesenchymal Stem Cells.
33803805	3	36	theme	enzyme	550:555	arg1	family					557:562	the same enzyme family	541:562	the same enzyme family as chitin synthases	541:582	Hyaluronan synthases (HAS) belong to the same enzyme family as chitin synthases.
33803805	10	37	theme	genes	1287:1291	arg1	expression					1273:1282	the expression	1269:1282	the expression of genes that encode proteins belonging to the HA biosynthesis and bone mineralization pathways	1269:1378	Fluid shear stress upregulated the expression of genes that encode proteins belonging to the HA biosynthesis and bone mineralization pathways.
33803805	12	38	from	role	1604:1607	arg1	microenvironment					1627:1642	the bone microenvironment	1618:1642	the bone microenvironment	1618:1642	Knowledge about the regulation mechanism governing HAS expression, trafficking, enzymatic activation and quality of the HA product in hMSCs is essential to understand the biological role of HA in the bone microenvironment.
33803805	7	39	theme	RNA	977:979	arg1	transcriptome					981:993	The RNA transcriptome	973:993	The RNA transcriptome	973:993	The RNA transcriptome was analyzed by RNA sequencing (RNAseq).
33803805	5	40	theme	molluscan	828:836	arg1	synthases					845:853	molluscan chitin synthases	828:853	molluscan chitin synthases	828:853	We hypothesize that HASes' expression and activity are controlled by fluid-induced mechanotransduction as it is known for molluscan chitin synthases.
33803805	12	41	theme	bone	1622:1625	arg1	microenvironment					1627:1642	the bone microenvironment	1618:1642	the bone microenvironment	1618:1642	Knowledge about the regulation mechanism governing HAS expression, trafficking, enzymatic activation and quality of the HA product in hMSCs is essential to understand the biological role of HA in the bone microenvironment.
33803805	1	42	theme	intracellular	301:313	arg1	composition					323:333	their intracellular protein composition	295:333	their intracellular protein composition according to these stimuli	295:360	During biomineralization, the cells generating the biominerals must be able to sense the external physical stimuli exerted by the growing mineralized tissue and change their intracellular protein composition according to these stimuli.
33803805	9	43	theme	confocal	1217:1224	arg1	microscopy					1226:1235	confocal microscopy	1217:1235	confocal microscopy	1217:1235	The cellular structure of hMSCs and HAS2-overexpressing hMSCs was investigated after treatment with shear stress using confocal microscopy.
33803805	3	44	theme	chitin	567:572	arg1	synthases					574:582	chitin synthases	567:582	chitin synthases	567:582	Hyaluronan synthases (HAS) belong to the same enzyme family as chitin synthases.
33803805	6	45	theme	marrow-derived	876:889	arg1	hMSCs					921:925	hMSCs	921:925	hMSCs	921:925	In this study, bone marrow-derived human mesenchymal stem cells (hMSCs) were exposed to fluid shear stress of 10 Pa.
33803805	6	45	theme	marrow-derived	876:889	arg1	cells					914:918	bone marrow-derived human mesenchymal stem cells	871:918	bone marrow-derived human mesenchymal stem cells (hMSCs)	871:926	In this study, bone marrow-derived human mesenchymal stem cells (hMSCs) were exposed to fluid shear stress of 10 Pa.
33803805	1	46	theme	protein	315:321	arg1	composition					323:333	their intracellular protein composition	295:333	their intracellular protein composition according to these stimuli	295:360	During biomineralization, the cells generating the biominerals must be able to sense the external physical stimuli exerted by the growing mineralized tissue and change their intracellular protein composition according to these stimuli.
33803805	7	47	theme	RNA	1011:1013	arg1	RNAseq					1027:1032	RNAseq	1027:1032	RNAseq	1027:1032	The RNA transcriptome was analyzed by RNA sequencing (RNAseq).
33803805	7	47	theme	RNA	1011:1013	arg1	sequencing					1015:1024	RNA sequencing	1011:1024	RNA sequencing (RNAseq)	1011:1033	The RNA transcriptome was analyzed by RNA sequencing (RNAseq).
33803805	8	48	from	concentrations	1039:1052	arg1	supernatants					1061:1072	the supernatants	1057:1072	the supernatants	1057:1072	HA concentrations in the supernatants were measured by ELISA.
33803805	12	49	theme	regulation	1442:1451	arg1	mechanism					1453:1461	the regulation mechanism	1438:1461	the regulation mechanism governing HAS expression, trafficking, enzymatic activation and quality of the HA product in hMSCs	1438:1560	Knowledge about the regulation mechanism governing HAS expression, trafficking, enzymatic activation and quality of the HA product in hMSCs is essential to understand the biological role of HA in the bone microenvironment.
33803805	12	50	theme	product	1545:1551	arg1	expression					1477:1486	expression	1477:1486	expression	1477:1486	Knowledge about the regulation mechanism governing HAS expression, trafficking, enzymatic activation and quality of the HA product in hMSCs is essential to understand the biological role of HA in the bone microenvironment.
33803805	12	50	theme	product	1545:1551	arg1	quality					1527:1533	quality	1527:1533	quality	1527:1533	Knowledge about the regulation mechanism governing HAS expression, trafficking, enzymatic activation and quality of the HA product in hMSCs is essential to understand the biological role of HA in the bone microenvironment.
33803805	12	50	theme	product	1545:1551	arg1	trafficking					1489:1499	trafficking	1489:1499	trafficking	1489:1499	Knowledge about the regulation mechanism governing HAS expression, trafficking, enzymatic activation and quality of the HA product in hMSCs is essential to understand the biological role of HA in the bone microenvironment.
33803805	12	50	theme	product	1545:1551	arg1	activation					1512:1521	enzymatic activation	1502:1521	enzymatic activation	1502:1521	Knowledge about the regulation mechanism governing HAS expression, trafficking, enzymatic activation and quality of the HA product in hMSCs is essential to understand the biological role of HA in the bone microenvironment.
33803805	12	51	theme	biological	1593:1602	arg1	role					1604:1607	the biological role	1589:1607	the biological role of HA in the bone microenvironment	1589:1642	Knowledge about the regulation mechanism governing HAS expression, trafficking, enzymatic activation and quality of the HA product in hMSCs is essential to understand the biological role of HA in the bone microenvironment.
33803805	9	52	theme	cellular	1102:1109	arg1	structure					1111:1119	The cellular structure	1098:1119	The cellular structure of hMSCs and HAS2-overexpressing hMSCs	1098:1158	The cellular structure of hMSCs and HAS2-overexpressing hMSCs was investigated after treatment with shear stress using confocal microscopy.
33803805	4	53	theme	bone	687:690	arg1	regeneration					692:703	bone regeneration	687:703	bone regeneration	687:703	Their product hyaluronan (HA) occurs in the bone and is supposed to have a regulatory function during bone regeneration.
33803805	1	54	theme	external	216:223	arg1	stimuli					234:240	the external physical stimuli	212:240	the external physical stimuli exerted by the growing mineralized tissue	212:282	During biomineralization, the cells generating the biominerals must be able to sense the external physical stimuli exerted by the growing mineralized tissue and change their intracellular protein composition according to these stimuli.
33803805	0	55	theme	Fluid	61:65	arg1	Stress					73:78	Fluid Shear Stress	61:78	Fluid Shear Stress in Bone Marrow-Derived Mesenchymal Stem Cells	61:124	Hyaluronan Synthases' Expression and Activity Are Induced by Fluid Shear Stress in Bone Marrow-Derived Mesenchymal Stem Cells.
33803805	0	56	dep	Synthases	11:19	arg1	Activity					37:44	Activity	37:44	Activity	37:44	Hyaluronan Synthases' Expression and Activity Are Induced by Fluid Shear Stress in Bone Marrow-Derived Mesenchymal Stem Cells.
33803805	0	56	dep	Synthases	11:19	arg1	Expression					22:31	Expression	22:31	Expression	22:31	Hyaluronan Synthases' Expression and Activity Are Induced by Fluid Shear Stress in Bone Marrow-Derived Mesenchymal Stem Cells.
33803805	0	56	dep	Synthases	11:19	arg1	Synthases					11:19	Hyaluronan Synthases	0:19	Hyaluronan Synthases' Expression and Activity	0:44	Hyaluronan Synthases' Expression and Activity Are Induced by Fluid Shear Stress in Bone Marrow-Derived Mesenchymal Stem Cells.
33803805	6	57	link	marrow-derived	876:889	arg1	hMSCs					921:925	hMSCs	921:925	hMSCs	921:925	In this study, bone marrow-derived human mesenchymal stem cells (hMSCs) were exposed to fluid shear stress of 10 Pa.
33803805	6	57	link	marrow-derived	876:889	arg1	cells					914:918	bone marrow-derived human mesenchymal stem cells	871:918	bone marrow-derived human mesenchymal stem cells (hMSCs)	871:926	In this study, bone marrow-derived human mesenchymal stem cells (hMSCs) were exposed to fluid shear stress of 10 Pa.
33803805	1	58	theme	physical	225:232	arg1	stimuli					234:240	the external physical stimuli	212:240	the external physical stimuli exerted by the growing mineralized tissue	212:282	During biomineralization, the cells generating the biominerals must be able to sense the external physical stimuli exerted by the growing mineralized tissue and change their intracellular protein composition according to these stimuli.
33803805	6	59	theme	stem	909:912	arg1	hMSCs					921:925	hMSCs	921:925	hMSCs	921:925	In this study, bone marrow-derived human mesenchymal stem cells (hMSCs) were exposed to fluid shear stress of 10 Pa.
33803805	6	59	theme	stem	909:912	arg1	cells					914:918	bone marrow-derived human mesenchymal stem cells	871:918	bone marrow-derived human mesenchymal stem cells (hMSCs)	871:926	In this study, bone marrow-derived human mesenchymal stem cells (hMSCs) were exposed to fluid shear stress of 10 Pa.
33803805	12	60	dep	governing	1463:1471	arg1	HAS					1473:1475	HAS	1473:1475	governing HAS expression, trafficking, enzymatic activation and quality of the HA product in hMSCs	1463:1560	Knowledge about the regulation mechanism governing HAS expression, trafficking, enzymatic activation and quality of the HA product in hMSCs is essential to understand the biological role of HA in the bone microenvironment.
33803805	10	61	theme	biosynthesis	1334:1345	arg1	pathways					1371:1378	the HA biosynthesis and bone mineralization pathways	1327:1378	the HA biosynthesis and bone mineralization pathways	1327:1378	Fluid shear stress upregulated the expression of genes that encode proteins belonging to the HA biosynthesis and bone mineralization pathways.
33803805	2	62	theme	molluscan	366:374	arg1	shell					376:380	molluscan shell	366:380	molluscan shell	366:380	In molluscan shell, the myosin-chitin synthases have been suggested to be the link for this communication between cells and the biomaterial.
33803805	10	63	theme	shear	1244:1248	arg1	stress					1250:1255	Fluid shear stress	1238:1255	Fluid shear stress	1238:1255	Fluid shear stress upregulated the expression of genes that encode proteins belonging to the HA biosynthesis and bone mineralization pathways.
33803805	6	64	theme	Pa.	969:971	arg1	stress					956:961	fluid shear stress	944:961	fluid shear stress of 10 Pa.	944:971	In this study, bone marrow-derived human mesenchymal stem cells (hMSCs) were exposed to fluid shear stress of 10 Pa.
33587171	3	0	theme	ZrO2	693:696	arg1	nanoparticles					698:710	the polymerized CS and ZrO2 nanoparticles	670:710	the polymerized CS and ZrO2 nanoparticles	670:710	Scanning electron microscope images showed a typical thin, wrinkled and fluctuant morphology of graphene nanosheets and the polymerized CS and ZrO2 nanoparticles deposited on the surface of rGOA.
33587171	8	1	theme	samples with	1489:1500	arg1	concentrations					1512:1525	spiked samples with different concentrations	1482:1525	spiked samples with different concentrations	1482:1525	Recoveries of spiked samples with different concentrations were in the range 78.6-103.3% with a relative RSD lower than 12.0%.
33587171	1	2	theme	chitosan/reduced	239:254	arg1	aerogel					271:277	the chitosan/reduced graphene oxide aerogel	235:277	the chitosan/reduced graphene oxide aerogel with dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor	235:392	A simple, fast and sensitive method for the detection of luteolin is proposed based on the chitosan/reduced graphene oxide aerogel with dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor.
33587171	4	3	theme	potential	924:932	arg1	-0.8-0.8 V					942:951	-0.8-0.8 V	942:951	-0.8-0.8 V	942:951	Cyclic voltammetry and differential pulse voltammetry were used to measure the electrochemical response of ZrO2/CS/rGOA composite-based biosensor towards luteolin at the working potential window (-0.8-0.8 V).
33587171	4	3	theme	potential	924:932	arg1	window					934:939	the working potential window	912:939	the working potential window (-0.8-0.8 V)	912:952	Cyclic voltammetry and differential pulse voltammetry were used to measure the electrochemical response of ZrO2/CS/rGOA composite-based biosensor towards luteolin at the working potential window (-0.8-0.8 V).
33587171	3	4	theme	Scanning	550:557	arg1	images					579:584	Scanning electron microscope images	550:584	Scanning electron microscope images	550:584	Scanning electron microscope images showed a typical thin, wrinkled and fluctuant morphology of graphene nanosheets and the polymerized CS and ZrO2 nanoparticles deposited on the surface of rGOA.
33587171	1	5	theme	oxide	265:269	arg1	aerogel					271:277	the chitosan/reduced graphene oxide aerogel	235:277	the chitosan/reduced graphene oxide aerogel with dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor	235:392	A simple, fast and sensitive method for the detection of luteolin is proposed based on the chitosan/reduced graphene oxide aerogel with dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor.
33587171	6	6	dep	1000 nM	1313:1319	arg1	to					1310:1311	to	1310:1311	to	1310:1311	Under optimized conditions, the analytical performance of this method towards luteolin was investigated with a detection limit of 1 nM and a linear range from 5 nM to 1000 nM.
33587171	4	7	theme	biosensor	882:890	arg1	response					841:848	the electrochemical response	821:848	the electrochemical response of ZrO2/CS/rGOA composite-based biosensor towards luteolin	821:907	Cyclic voltammetry and differential pulse voltammetry were used to measure the electrochemical response of ZrO2/CS/rGOA composite-based biosensor towards luteolin at the working potential window (-0.8-0.8 V).
33587171	7	8	theme	real	1454:1457	arg1	samples					1459:1465	real samples	1454:1465	real samples	1454:1465	Finally, the ZrO2/CS/rGOA-GCE electrochemical method coupled with solid phase extraction was used for the detection of luteolin in real samples.
33587171	6	9	theme	linear	1287:1292	arg1	range					1294:1298	a linear range	1285:1298	a linear range from 5 nM to 1000 nM	1285:1319	Under optimized conditions, the analytical performance of this method towards luteolin was investigated with a detection limit of 1 nM and a linear range from 5 nM to 1000 nM.
33587171	7	10	theme	luteolin	1442:1449	arg1	detection					1429:1437	the detection	1425:1437	the detection of luteolin in real samples	1425:1465	Finally, the ZrO2/CS/rGOA-GCE electrochemical method coupled with solid phase extraction was used for the detection of luteolin in real samples.
33587171	6	11	theme	analytical	1178:1187	arg1	performance					1189:1199	the analytical performance	1174:1199	the analytical performance of this method towards luteolin	1174:1231	Under optimized conditions, the analytical performance of this method towards luteolin was investigated with a detection limit of 1 nM and a linear range from 5 nM to 1000 nM.
33587171	0	12	theme	selective	115:123	arg1	detection					125:133	the selective detection	111:133	the selective detection of luteolin	111:145	Construction of an electrochemical sensor with graphene aerogel doped with ZrO2 nanoparticles and chitosan for the selective detection of luteolin.
33587171	5	13	theme	activity	1097:1104	arg1	transfer					1035:1042	efficient electron transfer	1016:1042	efficient electron transfer	1016:1042	The improved performance of this biosensor was attributed to efficient electron transfer and large surface area of 3D rGOA, and high specific activity of Zr towards adjacent hydroxyl groups.
33587171	5	13	theme	activity	1097:1104	arg1	area					1062:1065	large surface area	1048:1065	large surface area	1048:1065	The improved performance of this biosensor was attributed to efficient electron transfer and large surface area of 3D rGOA, and high specific activity of Zr towards adjacent hydroxyl groups.
33587171	3	14	theme	microscope	568:577	arg1	images					579:584	Scanning electron microscope images	550:584	Scanning electron microscope images	550:584	Scanning electron microscope images showed a typical thin, wrinkled and fluctuant morphology of graphene nanosheets and the polymerized CS and ZrO2 nanoparticles deposited on the surface of rGOA.
33587171	4	15	used	used	805:808	arg2	voltammetry					788:798	differential pulse voltammetry	769:798	differential pulse voltammetry	769:798	Cyclic voltammetry and differential pulse voltammetry were used to measure the electrochemical response of ZrO2/CS/rGOA composite-based biosensor towards luteolin at the working potential window (-0.8-0.8 V).
33587171	4	15	used	used	805:808	arg2	voltammetry					753:763	Cyclic voltammetry	746:763	Cyclic voltammetry	746:763	Cyclic voltammetry and differential pulse voltammetry were used to measure the electrochemical response of ZrO2/CS/rGOA composite-based biosensor towards luteolin at the working potential window (-0.8-0.8 V).
33587171	6	16	theme	1 nM	1276:1279	arg1	limit					1267:1271	a detection limit	1255:1271	a detection limit of 1 nM and a linear range from 5 nM to 1000 nM	1255:1319	Under optimized conditions, the analytical performance of this method towards luteolin was investigated with a detection limit of 1 nM and a linear range from 5 nM to 1000 nM.
33587171	5	17	theme	Zr	1109:1110	arg1	activity					1097:1104	high specific activity	1083:1104	high specific activity of Zr towards adjacent hydroxyl groups	1083:1143	The improved performance of this biosensor was attributed to efficient electron transfer and large surface area of 3D rGOA, and high specific activity of Zr towards adjacent hydroxyl groups.
33587171	5	17	theme	Zr	1109:1110	arg1	rGOA					1073:1076	3D rGOA	1070:1076	3D rGOA	1070:1076	The improved performance of this biosensor was attributed to efficient electron transfer and large surface area of 3D rGOA, and high specific activity of Zr towards adjacent hydroxyl groups.
33587171	1	18	dep	is proposed	214:224	arg1	based					226:230	based	226:230	is proposed based on the chitosan/reduced graphene oxide aerogel with dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor	214:392	A simple, fast and sensitive method for the detection of luteolin is proposed based on the chitosan/reduced graphene oxide aerogel with dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor.
33587171	7	19	used	used	1416:1419	arg2	method					1369:1374	the ZrO2/CS/rGOA-GCE electrochemical method	1332:1374	the ZrO2/CS/rGOA-GCE electrochemical method coupled with solid phase extraction	1332:1410	Finally, the ZrO2/CS/rGOA-GCE electrochemical method coupled with solid phase extraction was used for the detection of luteolin in real samples.
33587171	3	20	theme	typical	595:601	arg1	morphology					632:641	a typical thin, wrinkled and fluctuant morphology	593:641	a typical thin, wrinkled and fluctuant morphology of graphene nanosheets and the polymerized CS and ZrO2 nanoparticles	593:710	Scanning electron microscope images showed a typical thin, wrinkled and fluctuant morphology of graphene nanosheets and the polymerized CS and ZrO2 nanoparticles deposited on the surface of rGOA.
33587171	9	21	theme	graphene	1703:1710	arg1	aerogel					1712:1718	doped graphene aerogel	1697:1718	doped graphene aerogel	1697:1718	Graphical abstract Schematic representation of the preparation of the ZrO2 nanoparticles and chitosan doped graphene aerogel modified electrode.
33587171	7	22	from	detection	1429:1437	arg1	samples					1459:1465	real samples	1454:1465	real samples	1454:1465	Finally, the ZrO2/CS/rGOA-GCE electrochemical method coupled with solid phase extraction was used for the detection of luteolin in real samples.
33587171	8	23	theme	concentrations	1512:1525	arg1	Recoveries					1468:1477	Recoveries	1468:1477	Recoveries of spiked samples with different concentrations	1468:1525	Recoveries of spiked samples with different concentrations were in the range 78.6-103.3% with a relative RSD lower than 12.0%.
33587171	7	24	theme	phase	1395:1399	arg1	extraction					1401:1410	solid phase extraction	1389:1410	solid phase extraction	1389:1410	Finally, the ZrO2/CS/rGOA-GCE electrochemical method coupled with solid phase extraction was used for the detection of luteolin in real samples.
33587171	9	25	theme	Schematic	1614:1622	arg1	representation					1624:1637	Graphical abstract Schematic representation	1595:1637	Graphical abstract Schematic representation of the preparation of the ZrO2 nanoparticles	1595:1682	Graphical abstract Schematic representation of the preparation of the ZrO2 nanoparticles and chitosan doped graphene aerogel modified electrode.
33587171	5	26	theme	high	1083:1086	arg1	activity					1097:1104	high specific activity	1083:1104	high specific activity of Zr towards adjacent hydroxyl groups	1083:1143	The improved performance of this biosensor was attributed to efficient electron transfer and large surface area of 3D rGOA, and high specific activity of Zr towards adjacent hydroxyl groups.
33587171	2	27	theme	chloride	494:501	arg1	octahydrate					503:513	zirconyl chloride octahydrate	485:513	zirconyl chloride octahydrate	485:513	The ZrO2/CS/rGOA composite was prepared by one pot synthesis from a mixture of GO, CS and zirconyl chloride octahydrate, and subsequently be freeze-dried.
33587171	8	28	theme	range	1539:1543	arg1	%					1555:1555	the range 78.6-103.3%	1535:1555	the range 78.6-103.3% with a relative RSD lower than 12.0%	1535:1592	Recoveries of spiked samples with different concentrations were in the range 78.6-103.3% with a relative RSD lower than 12.0%.
33587171	0	29	theme	ZrO2	75:78	arg1	nanoparticles					80:92	ZrO2 nanoparticles	75:92	ZrO2 nanoparticles	75:92	Construction of an electrochemical sensor with graphene aerogel doped with ZrO2 nanoparticles and chitosan for the selective detection of luteolin.
33587171	3	30	dep	showed	586:591	arg1	deposited					712:720	deposited	712:720	showed a typical thin, wrinkled and fluctuant morphology of graphene nanosheets and the polymerized CS and ZrO2 nanoparticles deposited on the surface of rGOA	586:743	Scanning electron microscope images showed a typical thin, wrinkled and fluctuant morphology of graphene nanosheets and the polymerized CS and ZrO2 nanoparticles deposited on the surface of rGOA.
33587171	4	31	theme	pulse	782:786	arg1	voltammetry					788:798	differential pulse voltammetry	769:798	differential pulse voltammetry	769:798	Cyclic voltammetry and differential pulse voltammetry were used to measure the electrochemical response of ZrO2/CS/rGOA composite-based biosensor towards luteolin at the working potential window (-0.8-0.8 V).
33587171	5	32	theme	adjacent	1120:1127	arg1	groups					1138:1143	adjacent hydroxyl groups	1120:1143	adjacent hydroxyl groups	1120:1143	The improved performance of this biosensor was attributed to efficient electron transfer and large surface area of 3D rGOA, and high specific activity of Zr towards adjacent hydroxyl groups.
33587171	2	33	from	mixture	463:469	arg1	synthesis					446:454	one pot synthesis	438:454	one pot synthesis from a mixture of GO, CS and zirconyl chloride octahydrate	438:513	The ZrO2/CS/rGOA composite was prepared by one pot synthesis from a mixture of GO, CS and zirconyl chloride octahydrate, and subsequently be freeze-dried.
33587171	1	34	theme	electrochemical	371:385	arg1	sensor					387:392	an electrochemical sensor	368:392	an electrochemical sensor	368:392	A simple, fast and sensitive method for the detection of luteolin is proposed based on the chitosan/reduced graphene oxide aerogel with dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor.
33587171	5	35	theme	electron	1026:1033	arg1	transfer					1035:1042	efficient electron transfer	1016:1042	efficient electron transfer	1016:1042	The improved performance of this biosensor was attributed to efficient electron transfer and large surface area of 3D rGOA, and high specific activity of Zr towards adjacent hydroxyl groups.
33587171	4	36	theme	Cyclic	746:751	arg1	voltammetry					753:763	Cyclic voltammetry	746:763	Cyclic voltammetry	746:763	Cyclic voltammetry and differential pulse voltammetry were used to measure the electrochemical response of ZrO2/CS/rGOA composite-based biosensor towards luteolin at the working potential window (-0.8-0.8 V).
33587171	9	37	theme	abstract	1605:1612	arg1	representation					1624:1637	Graphical abstract Schematic representation	1595:1637	Graphical abstract Schematic representation of the preparation of the ZrO2 nanoparticles	1595:1682	Graphical abstract Schematic representation of the preparation of the ZrO2 nanoparticles and chitosan doped graphene aerogel modified electrode.
33587171	7	38	theme	electrochemical	1353:1367	arg1	method					1369:1374	the ZrO2/CS/rGOA-GCE electrochemical method	1332:1374	the ZrO2/CS/rGOA-GCE electrochemical method coupled with solid phase extraction	1332:1410	Finally, the ZrO2/CS/rGOA-GCE electrochemical method coupled with solid phase extraction was used for the detection of luteolin in real samples.
33587171	1	39	theme	dispersed	284:292	arg1	nanoparticles					299:311	dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE)	284:363	dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor	284:392	A simple, fast and sensitive method for the detection of luteolin is proposed based on the chitosan/reduced graphene oxide aerogel with dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor.
33587171	6	40	theme	method	1209:1214	arg1	performance					1189:1199	the analytical performance	1174:1199	the analytical performance of this method towards luteolin	1174:1231	Under optimized conditions, the analytical performance of this method towards luteolin was investigated with a detection limit of 1 nM and a linear range from 5 nM to 1000 nM.
33587171	0	41	theme	sensor	35:40	arg1	Construction					0:11	Construction	0:11	Construction of an electrochemical sensor with graphene	0:54	Construction of an electrochemical sensor with graphene aerogel doped with ZrO2 nanoparticles and chitosan for the selective detection of luteolin.
33587171	3	42	theme	graphene	646:653	arg1	nanosheets					655:664	graphene nanosheets	646:664	graphene nanosheets	646:664	Scanning electron microscope images showed a typical thin, wrinkled and fluctuant morphology of graphene nanosheets and the polymerized CS and ZrO2 nanoparticles deposited on the surface of rGOA.
33587171	4	43	theme	ZrO2/CS/rGOA	853:864	arg1	biosensor					882:890	ZrO2/CS/rGOA composite-based biosensor	853:890	ZrO2/CS/rGOA composite-based biosensor	853:890	Cyclic voltammetry and differential pulse voltammetry were used to measure the electrochemical response of ZrO2/CS/rGOA composite-based biosensor towards luteolin at the working potential window (-0.8-0.8 V).
33587171	1	44	theme	glassy	322:327	arg1	ZrO2/CS/rGOA-GCE					347:362	ZrO2/CS/rGOA-GCE	347:362	ZrO2/CS/rGOA-GCE	347:362	A simple, fast and sensitive method for the detection of luteolin is proposed based on the chitosan/reduced graphene oxide aerogel with dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor.
33587171	1	44	theme	glassy	322:327	arg1	electrode					336:344	modified glassy carbon electrode	313:344	dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor	284:392	A simple, fast and sensitive method for the detection of luteolin is proposed based on the chitosan/reduced graphene oxide aerogel with dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor.
33587171	5	45	theme	rGOA	1073:1076	arg1	transfer					1035:1042	efficient electron transfer	1016:1042	efficient electron transfer	1016:1042	The improved performance of this biosensor was attributed to efficient electron transfer and large surface area of 3D rGOA, and high specific activity of Zr towards adjacent hydroxyl groups.
33587171	5	45	theme	rGOA	1073:1076	arg1	area					1062:1065	large surface area	1048:1065	large surface area	1048:1065	The improved performance of this biosensor was attributed to efficient electron transfer and large surface area of 3D rGOA, and high specific activity of Zr towards adjacent hydroxyl groups.
33587171	1	46	theme	luteolin	205:212	arg1	detection					192:200	the detection	188:200	the detection of luteolin	188:212	A simple, fast and sensitive method for the detection of luteolin is proposed based on the chitosan/reduced graphene oxide aerogel with dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor.
33587171	9	47	theme	ZrO2	1665:1668	arg1	nanoparticles					1670:1682	the ZrO2 nanoparticles	1661:1682	the ZrO2 nanoparticles	1661:1682	Graphical abstract Schematic representation of the preparation of the ZrO2 nanoparticles and chitosan doped graphene aerogel modified electrode.
33587171	3	48	theme	polymerized	674:684	arg1	CS					686:687	the polymerized CS	670:687	the polymerized CS	670:687	Scanning electron microscope images showed a typical thin, wrinkled and fluctuant morphology of graphene nanosheets and the polymerized CS and ZrO2 nanoparticles deposited on the surface of rGOA.
33587171	10	49	theme	luteolin	1788:1795	arg1	detection					1775:1783	the detection	1771:1783	the detection of luteolin coupled with the solid-phase extraction technique	1771:1845	The electrode was employed for the detection of luteolin coupled with the solid-phase extraction technique.
33587171	6	50	from	1000 nM	1313:1319	arg1	limit					1267:1271	a detection limit	1255:1271	a detection limit of 1 nM and a linear range from 5 nM to 1000 nM	1255:1319	Under optimized conditions, the analytical performance of this method towards luteolin was investigated with a detection limit of 1 nM and a linear range from 5 nM to 1000 nM.
33587171	6	50	from	1000 nM	1313:1319	arg1	range					1294:1298	a linear range	1285:1298	a linear range from 5 nM to 1000 nM	1285:1319	Under optimized conditions, the analytical performance of this method towards luteolin was investigated with a detection limit of 1 nM and a linear range from 5 nM to 1000 nM.
33587171	6	50	from	1000 nM	1313:1319	arg1	1 nM					1276:1279	1 nM	1276:1279	1 nM	1276:1279	Under optimized conditions, the analytical performance of this method towards luteolin was investigated with a detection limit of 1 nM and a linear range from 5 nM to 1000 nM.
33587171	5	51	theme	surface	1054:1060	arg1	area					1062:1065	large surface area	1048:1065	large surface area	1048:1065	The improved performance of this biosensor was attributed to efficient electron transfer and large surface area of 3D rGOA, and high specific activity of Zr towards adjacent hydroxyl groups.
33587171	0	52	with	sensor	35:40	arg1	graphene					47:54	graphene	47:54	graphene	47:54	Construction of an electrochemical sensor with graphene aerogel doped with ZrO2 nanoparticles and chitosan for the selective detection of luteolin.
33587171	9	53	theme	doped	1697:1701	arg1	aerogel					1712:1718	doped graphene aerogel	1697:1718	doped graphene aerogel	1697:1718	Graphical abstract Schematic representation of the preparation of the ZrO2 nanoparticles and chitosan doped graphene aerogel modified electrode.
33587171	3	54	theme	nanoparticles	698:710	arg1	morphology					632:641	a typical thin, wrinkled and fluctuant morphology	593:641	a typical thin, wrinkled and fluctuant morphology of graphene nanosheets and the polymerized CS and ZrO2 nanoparticles	593:710	Scanning electron microscope images showed a typical thin, wrinkled and fluctuant morphology of graphene nanosheets and the polymerized CS and ZrO2 nanoparticles deposited on the surface of rGOA.
33587171	9	55	theme	preparation	1646:1656	arg1	chitosan					1688:1695	chitosan	1688:1695	chitosan	1688:1695	Graphical abstract Schematic representation of the preparation of the ZrO2 nanoparticles and chitosan doped graphene aerogel modified electrode.
33587171	9	55	theme	preparation	1646:1656	arg1	representation					1624:1637	Graphical abstract Schematic representation	1595:1637	Graphical abstract Schematic representation of the preparation of the ZrO2 nanoparticles	1595:1682	Graphical abstract Schematic representation of the preparation of the ZrO2 nanoparticles and chitosan doped graphene aerogel modified electrode.
33587171	10	56	theme	solid-phase	1814:1824	arg1	technique					1837:1845	the solid-phase extraction technique	1810:1845	the solid-phase extraction technique	1810:1845	The electrode was employed for the detection of luteolin coupled with the solid-phase extraction technique.
33587171	1	57	theme	graphene	256:263	arg1	aerogel					271:277	the chitosan/reduced graphene oxide aerogel	235:277	the chitosan/reduced graphene oxide aerogel with dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor	235:392	A simple, fast and sensitive method for the detection of luteolin is proposed based on the chitosan/reduced graphene oxide aerogel with dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor.
33587171	4	58	theme	working	916:922	arg1	-0.8-0.8 V					942:951	-0.8-0.8 V	942:951	-0.8-0.8 V	942:951	Cyclic voltammetry and differential pulse voltammetry were used to measure the electrochemical response of ZrO2/CS/rGOA composite-based biosensor towards luteolin at the working potential window (-0.8-0.8 V).
33587171	4	58	theme	working	916:922	arg1	window					934:939	the working potential window	912:939	the working potential window (-0.8-0.8 V)	912:952	Cyclic voltammetry and differential pulse voltammetry were used to measure the electrochemical response of ZrO2/CS/rGOA composite-based biosensor towards luteolin at the working potential window (-0.8-0.8 V).
33587171	3	59	theme	rGOA	740:743	arg1	surface					729:735	the surface	725:735	the surface of rGOA	725:743	Scanning electron microscope images showed a typical thin, wrinkled and fluctuant morphology of graphene nanosheets and the polymerized CS and ZrO2 nanoparticles deposited on the surface of rGOA.
33587171	6	60	theme	range	1294:1298	arg1	limit					1267:1271	a detection limit	1255:1271	a detection limit of 1 nM and a linear range from 5 nM to 1000 nM	1255:1319	Under optimized conditions, the analytical performance of this method towards luteolin was investigated with a detection limit of 1 nM and a linear range from 5 nM to 1000 nM.
33587171	1	61	theme	fast	158:161	arg1	method					177:182	A simple, fast and sensitive method	148:182	A simple, fast and sensitive method for the detection of luteolin	148:212	A simple, fast and sensitive method for the detection of luteolin is proposed based on the chitosan/reduced graphene oxide aerogel with dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor.
33587171	4	62	theme	composite-based	866:880	arg1	biosensor					882:890	ZrO2/CS/rGOA composite-based biosensor	853:890	ZrO2/CS/rGOA composite-based biosensor	853:890	Cyclic voltammetry and differential pulse voltammetry were used to measure the electrochemical response of ZrO2/CS/rGOA composite-based biosensor towards luteolin at the working potential window (-0.8-0.8 V).
33587171	1	63	theme	sensitive	167:175	arg1	method					177:182	A simple, fast and sensitive method	148:182	A simple, fast and sensitive method for the detection of luteolin	148:212	A simple, fast and sensitive method for the detection of luteolin is proposed based on the chitosan/reduced graphene oxide aerogel with dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor.
33587171	1	64	with	aerogel	271:277	arg1	nanoparticles					299:311	dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE)	284:363	dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor	284:392	A simple, fast and sensitive method for the detection of luteolin is proposed based on the chitosan/reduced graphene oxide aerogel with dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor.
33587171	3	65	theme	electron	559:566	arg1	images					579:584	Scanning electron microscope images	550:584	Scanning electron microscope images	550:584	Scanning electron microscope images showed a typical thin, wrinkled and fluctuant morphology of graphene nanosheets and the polymerized CS and ZrO2 nanoparticles deposited on the surface of rGOA.
33587171	8	66	theme	spiked	1482:1487	arg1	concentrations					1512:1525	spiked samples with different concentrations	1482:1525	spiked samples with different concentrations	1482:1525	Recoveries of spiked samples with different concentrations were in the range 78.6-103.3% with a relative RSD lower than 12.0%.
33587171	0	67	theme	luteolin	138:145	arg1	detection					125:133	the selective detection	111:133	the selective detection of luteolin	111:145	Construction of an electrochemical sensor with graphene aerogel doped with ZrO2 nanoparticles and chitosan for the selective detection of luteolin.
33587171	3	68	theme	thin	603:606	arg1	morphology					632:641	a typical thin, wrinkled and fluctuant morphology	593:641	a typical thin, wrinkled and fluctuant morphology of graphene nanosheets and the polymerized CS and ZrO2 nanoparticles	593:710	Scanning electron microscope images showed a typical thin, wrinkled and fluctuant morphology of graphene nanosheets and the polymerized CS and ZrO2 nanoparticles deposited on the surface of rGOA.
33587171	8	69	theme	different	1502:1510	arg1	concentrations					1512:1525	spiked samples with different concentrations	1482:1525	spiked samples with different concentrations	1482:1525	Recoveries of spiked samples with different concentrations were in the range 78.6-103.3% with a relative RSD lower than 12.0%.
33587171	6	70	theme	detection	1257:1265	arg1	limit					1267:1271	a detection limit	1255:1271	a detection limit of 1 nM and a linear range from 5 nM to 1000 nM	1255:1319	Under optimized conditions, the analytical performance of this method towards luteolin was investigated with a detection limit of 1 nM and a linear range from 5 nM to 1000 nM.
33587171	6	71	from	limit	1267:1271	arg1	1000 nM					1313:1319	1000 nM	1313:1319	1000 nM	1313:1319	Under optimized conditions, the analytical performance of this method towards luteolin was investigated with a detection limit of 1 nM and a linear range from 5 nM to 1000 nM.
33587171	3	72	theme	fluctuant	622:630	arg1	morphology					632:641	a typical thin, wrinkled and fluctuant morphology	593:641	a typical thin, wrinkled and fluctuant morphology of graphene nanosheets and the polymerized CS and ZrO2 nanoparticles	593:710	Scanning electron microscope images showed a typical thin, wrinkled and fluctuant morphology of graphene nanosheets and the polymerized CS and ZrO2 nanoparticles deposited on the surface of rGOA.
33587171	5	73	theme	improved	959:966	arg1	performance					968:978	The improved performance	955:978	The improved performance of this biosensor	955:996	The improved performance of this biosensor was attributed to efficient electron transfer and large surface area of 3D rGOA, and high specific activity of Zr towards adjacent hydroxyl groups.
33587171	5	74	theme	specific	1088:1095	arg1	activity					1097:1104	high specific activity	1083:1104	high specific activity of Zr towards adjacent hydroxyl groups	1083:1143	The improved performance of this biosensor was attributed to efficient electron transfer and large surface area of 3D rGOA, and high specific activity of Zr towards adjacent hydroxyl groups.
33587171	3	75	theme	wrinkled	609:616	arg1	morphology					632:641	a typical thin, wrinkled and fluctuant morphology	593:641	a typical thin, wrinkled and fluctuant morphology of graphene nanosheets and the polymerized CS and ZrO2 nanoparticles	593:710	Scanning electron microscope images showed a typical thin, wrinkled and fluctuant morphology of graphene nanosheets and the polymerized CS and ZrO2 nanoparticles deposited on the surface of rGOA.
33587171	2	76	theme	zirconyl	485:492	arg1	octahydrate					503:513	zirconyl chloride octahydrate	485:513	zirconyl chloride octahydrate	485:513	The ZrO2/CS/rGOA composite was prepared by one pot synthesis from a mixture of GO, CS and zirconyl chloride octahydrate, and subsequently be freeze-dried.
33587171	7	77	theme	solid	1389:1393	arg1	extraction					1401:1410	solid phase extraction	1389:1410	solid phase extraction	1389:1410	Finally, the ZrO2/CS/rGOA-GCE electrochemical method coupled with solid phase extraction was used for the detection of luteolin in real samples.
33587171	9	78	dep	representation	1624:1637	arg1	aerogel					1712:1718	doped graphene aerogel	1697:1718	doped graphene aerogel	1697:1718	Graphical abstract Schematic representation of the preparation of the ZrO2 nanoparticles and chitosan doped graphene aerogel modified electrode.
33587171	1	79	theme	simple	150:155	arg1	method					177:182	A simple, fast and sensitive method	148:182	A simple, fast and sensitive method for the detection of luteolin	148:212	A simple, fast and sensitive method for the detection of luteolin is proposed based on the chitosan/reduced graphene oxide aerogel with dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor.
33587171	2	80	theme	CS	478:479	arg1	mixture					463:469	a mixture	461:469	a mixture of GO, CS and zirconyl chloride octahydrate	461:513	The ZrO2/CS/rGOA composite was prepared by one pot synthesis from a mixture of GO, CS and zirconyl chloride octahydrate, and subsequently be freeze-dried.
33587171	10	81	theme	extraction	1826:1835	arg1	technique					1837:1845	the solid-phase extraction technique	1810:1845	the solid-phase extraction technique	1810:1845	The electrode was employed for the detection of luteolin coupled with the solid-phase extraction technique.
33587171	8	82	theme	relative	1564:1571	arg1	RSD					1573:1575	a relative RSD	1562:1575	a relative RSD lower than 12.0%	1562:1592	Recoveries of spiked samples with different concentrations were in the range 78.6-103.3% with a relative RSD lower than 12.0%.
33587171	4	83	theme	differential	769:780	arg1	voltammetry					788:798	differential pulse voltammetry	769:798	differential pulse voltammetry	769:798	Cyclic voltammetry and differential pulse voltammetry were used to measure the electrochemical response of ZrO2/CS/rGOA composite-based biosensor towards luteolin at the working potential window (-0.8-0.8 V).
33587171	5	84	theme	hydroxyl	1129:1136	arg1	groups					1138:1143	adjacent hydroxyl groups	1120:1143	adjacent hydroxyl groups	1120:1143	The improved performance of this biosensor was attributed to efficient electron transfer and large surface area of 3D rGOA, and high specific activity of Zr towards adjacent hydroxyl groups.
33587171	5	85	theme	efficient	1016:1024	arg1	transfer					1035:1042	efficient electron transfer	1016:1042	efficient electron transfer	1016:1042	The improved performance of this biosensor was attributed to efficient electron transfer and large surface area of 3D rGOA, and high specific activity of Zr towards adjacent hydroxyl groups.
33587171	2	86	theme	octahydrate	503:513	arg1	mixture					463:469	a mixture	461:469	a mixture of GO, CS and zirconyl chloride octahydrate	461:513	The ZrO2/CS/rGOA composite was prepared by one pot synthesis from a mixture of GO, CS and zirconyl chloride octahydrate, and subsequently be freeze-dried.
33587171	1	87	dep	nanoparticles	299:311	arg1	ZrO2/CS/rGOA-GCE					347:362	ZrO2/CS/rGOA-GCE	347:362	ZrO2/CS/rGOA-GCE	347:362	A simple, fast and sensitive method for the detection of luteolin is proposed based on the chitosan/reduced graphene oxide aerogel with dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor.
33587171	1	87	dep	nanoparticles	299:311	arg1	electrode					336:344	modified glassy carbon electrode	313:344	dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor	284:392	A simple, fast and sensitive method for the detection of luteolin is proposed based on the chitosan/reduced graphene oxide aerogel with dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor.
33587171	2	88	theme	ZrO2/CS/rGOA	399:410	arg1	composite					412:420	The ZrO2/CS/rGOA composite	395:420	The ZrO2/CS/rGOA composite	395:420	The ZrO2/CS/rGOA composite was prepared by one pot synthesis from a mixture of GO, CS and zirconyl chloride octahydrate, and subsequently be freeze-dried.
33587171	9	89	theme	Graphical	1595:1603	arg1	representation					1624:1637	Graphical abstract Schematic representation	1595:1637	Graphical abstract Schematic representation of the preparation of the ZrO2 nanoparticles	1595:1682	Graphical abstract Schematic representation of the preparation of the ZrO2 nanoparticles and chitosan doped graphene aerogel modified electrode.
33587171	7	90	theme	ZrO2/CS/rGOA-GCE	1336:1351	arg1	method					1369:1374	the ZrO2/CS/rGOA-GCE electrochemical method	1332:1374	the ZrO2/CS/rGOA-GCE electrochemical method coupled with solid phase extraction	1332:1410	Finally, the ZrO2/CS/rGOA-GCE electrochemical method coupled with solid phase extraction was used for the detection of luteolin in real samples.
33587171	0	91	theme	electrochemical	19:33	arg1	sensor					35:40	an electrochemical sensor	16:40	an electrochemical sensor with graphene	16:54	Construction of an electrochemical sensor with graphene aerogel doped with ZrO2 nanoparticles and chitosan for the selective detection of luteolin.
33587171	9	92	theme	nanoparticles	1670:1682	arg1	preparation					1646:1656	the preparation	1642:1656	the preparation of the ZrO2 nanoparticles	1642:1682	Graphical abstract Schematic representation of the preparation of the ZrO2 nanoparticles and chitosan doped graphene aerogel modified electrode.
33587171	5	93	theme	biosensor	988:996	arg1	performance					968:978	The improved performance	955:978	The improved performance of this biosensor	955:996	The improved performance of this biosensor was attributed to efficient electron transfer and large surface area of 3D rGOA, and high specific activity of Zr towards adjacent hydroxyl groups.
33587171	2	94	theme	pot	442:444	arg1	synthesis					446:454	one pot synthesis	438:454	one pot synthesis from a mixture of GO, CS and zirconyl chloride octahydrate	438:513	The ZrO2/CS/rGOA composite was prepared by one pot synthesis from a mixture of GO, CS and zirconyl chloride octahydrate, and subsequently be freeze-dried.
33587171	3	95	theme	nanosheets	655:664	arg1	morphology					632:641	a typical thin, wrinkled and fluctuant morphology	593:641	a typical thin, wrinkled and fluctuant morphology of graphene nanosheets and the polymerized CS and ZrO2 nanoparticles	593:710	Scanning electron microscope images showed a typical thin, wrinkled and fluctuant morphology of graphene nanosheets and the polymerized CS and ZrO2 nanoparticles deposited on the surface of rGOA.
33587171	1	96	theme	ZrO2	294:297	arg1	nanoparticles					299:311	dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE)	284:363	dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor	284:392	A simple, fast and sensitive method for the detection of luteolin is proposed based on the chitosan/reduced graphene oxide aerogel with dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor.
33587171	0	97	dep	aerogel	56:62	arg1	doped					64:68	doped	64:68	aerogel doped with ZrO2 nanoparticles and chitosan for the selective detection of luteolin	56:145	Construction of an electrochemical sensor with graphene aerogel doped with ZrO2 nanoparticles and chitosan for the selective detection of luteolin.
33587171	2	98	theme	GO	474:475	arg1	mixture					463:469	a mixture	461:469	a mixture of GO, CS and zirconyl chloride octahydrate	461:513	The ZrO2/CS/rGOA composite was prepared by one pot synthesis from a mixture of GO, CS and zirconyl chloride octahydrate, and subsequently be freeze-dried.
33587171	4	99	theme	electrochemical	825:839	arg1	response					841:848	the electrochemical response	821:848	the electrochemical response of ZrO2/CS/rGOA composite-based biosensor towards luteolin	821:907	Cyclic voltammetry and differential pulse voltammetry were used to measure the electrochemical response of ZrO2/CS/rGOA composite-based biosensor towards luteolin at the working potential window (-0.8-0.8 V).
33587171	1	100	theme	modified	313:320	arg1	ZrO2/CS/rGOA-GCE					347:362	ZrO2/CS/rGOA-GCE	347:362	ZrO2/CS/rGOA-GCE	347:362	A simple, fast and sensitive method for the detection of luteolin is proposed based on the chitosan/reduced graphene oxide aerogel with dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor.
33587171	1	100	theme	modified	313:320	arg1	electrode					336:344	modified glassy carbon electrode	313:344	dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor	284:392	A simple, fast and sensitive method for the detection of luteolin is proposed based on the chitosan/reduced graphene oxide aerogel with dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor.
33587171	5	101	theme	3D	1070:1071	arg1	rGOA					1073:1076	3D rGOA	1070:1076	3D rGOA	1070:1076	The improved performance of this biosensor was attributed to efficient electron transfer and large surface area of 3D rGOA, and high specific activity of Zr towards adjacent hydroxyl groups.
33587171	8	102	theme	lower	1577:1581	arg1	RSD					1573:1575	a relative RSD	1562:1575	a relative RSD lower than 12.0%	1562:1592	Recoveries of spiked samples with different concentrations were in the range 78.6-103.3% with a relative RSD lower than 12.0%.
33587171	3	103	theme	CS	686:687	arg1	nanoparticles					698:710	the polymerized CS and ZrO2 nanoparticles	670:710	the polymerized CS and ZrO2 nanoparticles	670:710	Scanning electron microscope images showed a typical thin, wrinkled and fluctuant morphology of graphene nanosheets and the polymerized CS and ZrO2 nanoparticles deposited on the surface of rGOA.
33587171	1	104	theme	carbon	329:334	arg1	ZrO2/CS/rGOA-GCE					347:362	ZrO2/CS/rGOA-GCE	347:362	ZrO2/CS/rGOA-GCE	347:362	A simple, fast and sensitive method for the detection of luteolin is proposed based on the chitosan/reduced graphene oxide aerogel with dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor.
33587171	1	104	theme	carbon	329:334	arg1	electrode					336:344	modified glassy carbon electrode	313:344	dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor	284:392	A simple, fast and sensitive method for the detection of luteolin is proposed based on the chitosan/reduced graphene oxide aerogel with dispersed ZrO2 nanoparticles modified glassy carbon electrode (ZrO2/CS/rGOA-GCE) as an electrochemical sensor.
33587171	6	105	theme	optimized	1152:1160	arg1	conditions					1162:1171	optimized conditions	1152:1171	optimized conditions	1152:1171	Under optimized conditions, the analytical performance of this method towards luteolin was investigated with a detection limit of 1 nM and a linear range from 5 nM to 1000 nM.
33587171	5	106	theme	large	1048:1052	arg1	area					1062:1065	large surface area	1048:1065	large surface area	1048:1065	The improved performance of this biosensor was attributed to efficient electron transfer and large surface area of 3D rGOA, and high specific activity of Zr towards adjacent hydroxyl groups.
33587171	8	107	with	%	1555:1555	arg1	RSD					1573:1575	a relative RSD	1562:1575	a relative RSD lower than 12.0%	1562:1592	Recoveries of spiked samples with different concentrations were in the range 78.6-103.3% with a relative RSD lower than 12.0%.
32125157	2	0	theme	individual	506:515	arg1	isomers					517:523	individual isomers	506:523	individual isomers	506:523	The effects and mechanism of CLNA crude extract and individual isomers on colitis were explored.
32125157	5	1	theme	cytokine	1018:1025	arg1	IL-10					1027:1031	the colonic anti-inflammatory cytokine IL-10	988:1031	the colonic anti-inflammatory cytokine IL-10	988:1031	CLNA significantly attenuated the level of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) while upregulating the expression of the colonic anti-inflammatory cytokine IL-10 and nuclear receptor peroxisome-activated receptor-γ.
32125157	3	2	theme	colon	625:629	arg1	shortening					631:640	colon shortening	625:640	colon shortening	625:640	CLNA significantly inhibited weight loss, the disease activity index, and colon shortening.
32125157	2	3	from	mechanism	470:478	arg1	colitis					528:534	colitis	528:534	colitis	528:534	The effects and mechanism of CLNA crude extract and individual isomers on colitis were explored.
32125157	5	4	theme	nuclear	1037:1043	arg1	receptor-γ					1075:1084	nuclear receptor peroxisome-activated receptor-γ	1037:1084	nuclear receptor peroxisome-activated receptor-γ	1037:1084	CLNA significantly attenuated the level of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) while upregulating the expression of the colonic anti-inflammatory cytokine IL-10 and nuclear receptor peroxisome-activated receptor-γ.
32125157	4	5	theme	tight	786:790	arg1	occludin					817:824	occludin	817:824	occludin	817:824	Additionally, CLNA alleviated histological damage, protected colonic mucus layer integrity, and significantly upregulated the concentration of tight junction proteins (ZO-1, occludin, E-cadherin 1, and claudin-3).
32125157	4	5	theme	tight	786:790	arg1	claudin-3					845:853	claudin-3	845:853	claudin-3	845:853	Additionally, CLNA alleviated histological damage, protected colonic mucus layer integrity, and significantly upregulated the concentration of tight junction proteins (ZO-1, occludin, E-cadherin 1, and claudin-3).
32125157	4	5	theme	tight	786:790	arg1	proteins					801:808	tight junction proteins	786:808	tight junction proteins (ZO-1, occludin, E-cadherin 1, and claudin-3)	786:854	Additionally, CLNA alleviated histological damage, protected colonic mucus layer integrity, and significantly upregulated the concentration of tight junction proteins (ZO-1, occludin, E-cadherin 1, and claudin-3).
32125157	4	5	theme	tight	786:790	arg1	ZO-1					811:814	ZO-1	811:814	ZO-1	811:814	Additionally, CLNA alleviated histological damage, protected colonic mucus layer integrity, and significantly upregulated the concentration of tight junction proteins (ZO-1, occludin, E-cadherin 1, and claudin-3).
32125157	4	5	theme	tight	786:790	arg1	E-cadherin					827:836	E-cadherin 1	827:838	E-cadherin 1	827:838	Additionally, CLNA alleviated histological damage, protected colonic mucus layer integrity, and significantly upregulated the concentration of tight junction proteins (ZO-1, occludin, E-cadherin 1, and claudin-3).
32125157	4	6	theme	proteins	801:808	arg1	concentration					769:781	the concentration	765:781	the concentration of tight junction proteins (ZO-1, occludin, E-cadherin 1, and claudin-3)	765:854	Additionally, CLNA alleviated histological damage, protected colonic mucus layer integrity, and significantly upregulated the concentration of tight junction proteins (ZO-1, occludin, E-cadherin 1, and claudin-3).
32125157	4	7	theme	junction	792:799	arg1	occludin					817:824	occludin	817:824	occludin	817:824	Additionally, CLNA alleviated histological damage, protected colonic mucus layer integrity, and significantly upregulated the concentration of tight junction proteins (ZO-1, occludin, E-cadherin 1, and claudin-3).
32125157	4	7	theme	junction	792:799	arg1	claudin-3					845:853	claudin-3	845:853	claudin-3	845:853	Additionally, CLNA alleviated histological damage, protected colonic mucus layer integrity, and significantly upregulated the concentration of tight junction proteins (ZO-1, occludin, E-cadherin 1, and claudin-3).
32125157	4	7	theme	junction	792:799	arg1	proteins					801:808	tight junction proteins	786:808	tight junction proteins (ZO-1, occludin, E-cadherin 1, and claudin-3)	786:854	Additionally, CLNA alleviated histological damage, protected colonic mucus layer integrity, and significantly upregulated the concentration of tight junction proteins (ZO-1, occludin, E-cadherin 1, and claudin-3).
32125157	4	7	theme	junction	792:799	arg1	ZO-1					811:814	ZO-1	811:814	ZO-1	811:814	Additionally, CLNA alleviated histological damage, protected colonic mucus layer integrity, and significantly upregulated the concentration of tight junction proteins (ZO-1, occludin, E-cadherin 1, and claudin-3).
32125157	4	7	theme	junction	792:799	arg1	E-cadherin					827:836	E-cadherin 1	827:838	E-cadherin 1	827:838	Additionally, CLNA alleviated histological damage, protected colonic mucus layer integrity, and significantly upregulated the concentration of tight junction proteins (ZO-1, occludin, E-cadherin 1, and claudin-3).
32125157	0	8	theme	plantarum	59:67	arg1	Colitis					118:124	Lactobacillus plantarum ZS2058 Ameliorate Dextran Sodium Sulfate-Induced Colitis	45:124	Lactobacillus plantarum ZS2058 Ameliorate Dextran Sodium Sulfate-Induced Colitis in Mice	45:132	c9, t11, c15-CLNA and t9, t11, c15-CLNA from Lactobacillus plantarum ZS2058 Ameliorate Dextran Sodium Sulfate-Induced Colitis in Mice.
32125157	3	9	theme	disease	597:603	arg1	index					614:618	the disease activity index	593:618	the disease activity index	593:618	CLNA significantly inhibited weight loss, the disease activity index, and colon shortening.
32125157	2	10	theme	extract	494:500	arg1	mechanism					470:478	mechanism	470:478	mechanism	470:478	The effects and mechanism of CLNA crude extract and individual isomers on colitis were explored.
32125157	2	10	theme	extract	494:500	arg1	effects					458:464	effects	458:464	effects	458:464	The effects and mechanism of CLNA crude extract and individual isomers on colitis were explored.
32125157	4	11	theme	histological	673:684	arg1	damage					686:691	histological damage	673:691	histological damage	673:691	Additionally, CLNA alleviated histological damage, protected colonic mucus layer integrity, and significantly upregulated the concentration of tight junction proteins (ZO-1, occludin, E-cadherin 1, and claudin-3).
32125157	1	12	theme	probiotic	222:230	arg1	bacterium					232:240	the probiotic bacterium	218:240	the probiotic bacterium	218:240	To investigate the specific functions of conjugated fatty acids (CFAs) produced by the probiotic bacterium, α-linolenic acid was isomerized by Lactobacillus plantarum ZS2058, and two different conjugated linolenic acid (CLNA) isomers were successfully isolated: c9, t11, c15-CLNA (CLNA1) and t9, t11, c15-CLNA (CLNA2).
32125157	0	13	theme	Lactobacillus	45:57	arg1	Colitis					118:124	Lactobacillus plantarum ZS2058 Ameliorate Dextran Sodium Sulfate-Induced Colitis	45:124	Lactobacillus plantarum ZS2058 Ameliorate Dextran Sodium Sulfate-Induced Colitis in Mice	45:132	c9, t11, c15-CLNA and t9, t11, c15-CLNA from Lactobacillus plantarum ZS2058 Ameliorate Dextran Sodium Sulfate-Induced Colitis in Mice.
32125157	1	14	theme	different	318:326	arg1	isomers					361:367	two different conjugated linolenic acid (CLNA) isomers	314:367	two different conjugated linolenic acid (CLNA) isomers	314:367	To investigate the specific functions of conjugated fatty acids (CFAs) produced by the probiotic bacterium, α-linolenic acid was isomerized by Lactobacillus plantarum ZS2058, and two different conjugated linolenic acid (CLNA) isomers were successfully isolated: c9, t11, c15-CLNA (CLNA1) and t9, t11, c15-CLNA (CLNA2).
32125157	2	15	theme	crude	488:492	arg1	extract					494:500	CLNA crude extract	483:500	CLNA crude extract	483:500	The effects and mechanism of CLNA crude extract and individual isomers on colitis were explored.
32125157	7	16	theme	colonic	1339:1345	arg1	content					1347:1353	the colonic content	1335:1353	the colonic content	1335:1353	Meanwhile, the concentrations of CLNA in the liver and conjugated linoleic acid in the colonic content were significantly increased because of the treatment of CLNA.
32125157	7	17	from	Meanwhile	1252:1260	arg1	content					1347:1353	the colonic content	1335:1353	the colonic content	1335:1353	Meanwhile, the concentrations of CLNA in the liver and conjugated linoleic acid in the colonic content were significantly increased because of the treatment of CLNA.
32125157	7	17	from	Meanwhile	1252:1260	arg1	liver					1297:1301	the liver	1293:1301	the liver	1293:1301	Meanwhile, the concentrations of CLNA in the liver and conjugated linoleic acid in the colonic content were significantly increased because of the treatment of CLNA.
32125157	7	18	from	concentrations	1267:1280	arg1	content					1347:1353	the colonic content	1335:1353	the colonic content	1335:1353	Meanwhile, the concentrations of CLNA in the liver and conjugated linoleic acid in the colonic content were significantly increased because of the treatment of CLNA.
32125157	7	18	from	concentrations	1267:1280	arg1	liver					1297:1301	the liver	1293:1301	the liver	1293:1301	Meanwhile, the concentrations of CLNA in the liver and conjugated linoleic acid in the colonic content were significantly increased because of the treatment of CLNA.
32125157	0	19	theme	Ameliorate	76:85	arg1	Colitis					118:124	Lactobacillus plantarum ZS2058 Ameliorate Dextran Sodium Sulfate-Induced Colitis	45:124	Lactobacillus plantarum ZS2058 Ameliorate Dextran Sodium Sulfate-Induced Colitis in Mice	45:132	c9, t11, c15-CLNA and t9, t11, c15-CLNA from Lactobacillus plantarum ZS2058 Ameliorate Dextran Sodium Sulfate-Induced Colitis in Mice.
32125157	1	20	theme	conjugated	328:337	arg1	CLNA					355:358	CLNA	355:358	CLNA	355:358	To investigate the specific functions of conjugated fatty acids (CFAs) produced by the probiotic bacterium, α-linolenic acid was isomerized by Lactobacillus plantarum ZS2058, and two different conjugated linolenic acid (CLNA) isomers were successfully isolated: c9, t11, c15-CLNA (CLNA1) and t9, t11, c15-CLNA (CLNA2).
32125157	1	20	theme	conjugated	328:337	arg1	acid					349:352	conjugated linolenic acid	328:352	two different conjugated linolenic acid (CLNA) isomers	314:367	To investigate the specific functions of conjugated fatty acids (CFAs) produced by the probiotic bacterium, α-linolenic acid was isomerized by Lactobacillus plantarum ZS2058, and two different conjugated linolenic acid (CLNA) isomers were successfully isolated: c9, t11, c15-CLNA (CLNA1) and t9, t11, c15-CLNA (CLNA2).
32125157	9	21	theme	Ruminococcus	1587:1598	arg1	abundance					1574:1582	the abundance	1570:1582	the abundance of Ruminococcus and Prevotella	1570:1613	CLNA1 and CLNA2 increased the abundance of Ruminococcus and Prevotella, respectively.
32125157	6	22	theme	oxidative	1128:1136	arg1	GSH					1167:1169	GSH	1167:1169	GSH	1167:1169	Moreover, CLNA increased the activity of oxidative stress-related enzymes (SOD, GSH, and CAT), and the myeloperoxidase activity was significantly decreased by CLNA.
32125157	6	22	theme	oxidative	1128:1136	arg1	CAT					1176:1178	CAT	1176:1178	CAT	1176:1178	Moreover, CLNA increased the activity of oxidative stress-related enzymes (SOD, GSH, and CAT), and the myeloperoxidase activity was significantly decreased by CLNA.
32125157	6	22	theme	oxidative	1128:1136	arg1	SOD					1162:1164	SOD	1162:1164	SOD	1162:1164	Moreover, CLNA increased the activity of oxidative stress-related enzymes (SOD, GSH, and CAT), and the myeloperoxidase activity was significantly decreased by CLNA.
32125157	6	22	theme	oxidative	1128:1136	arg1	enzymes					1153:1159	oxidative stress-related enzymes	1128:1159	oxidative stress-related enzymes (SOD, GSH, and CAT)	1128:1179	Moreover, CLNA increased the activity of oxidative stress-related enzymes (SOD, GSH, and CAT), and the myeloperoxidase activity was significantly decreased by CLNA.
32125157	0	23	theme	ZS2058	69:74	arg1	Colitis					118:124	Lactobacillus plantarum ZS2058 Ameliorate Dextran Sodium Sulfate-Induced Colitis	45:124	Lactobacillus plantarum ZS2058 Ameliorate Dextran Sodium Sulfate-Induced Colitis in Mice	45:132	c9, t11, c15-CLNA and t9, t11, c15-CLNA from Lactobacillus plantarum ZS2058 Ameliorate Dextran Sodium Sulfate-Induced Colitis in Mice.
32125157	1	24	theme	linolenic	339:347	arg1	CLNA					355:358	CLNA	355:358	CLNA	355:358	To investigate the specific functions of conjugated fatty acids (CFAs) produced by the probiotic bacterium, α-linolenic acid was isomerized by Lactobacillus plantarum ZS2058, and two different conjugated linolenic acid (CLNA) isomers were successfully isolated: c9, t11, c15-CLNA (CLNA1) and t9, t11, c15-CLNA (CLNA2).
32125157	1	24	theme	linolenic	339:347	arg1	acid					349:352	conjugated linolenic acid	328:352	two different conjugated linolenic acid (CLNA) isomers	314:367	To investigate the specific functions of conjugated fatty acids (CFAs) produced by the probiotic bacterium, α-linolenic acid was isomerized by Lactobacillus plantarum ZS2058, and two different conjugated linolenic acid (CLNA) isomers were successfully isolated: c9, t11, c15-CLNA (CLNA1) and t9, t11, c15-CLNA (CLNA2).
32125157	2	25	dep	effects	458:464	arg1	The					454:456	The	454:456	The	454:456	The effects and mechanism of CLNA crude extract and individual isomers on colitis were explored.
32125157	1	26	theme	acid	349:352	arg1	isomers					361:367	two different conjugated linolenic acid (CLNA) isomers	314:367	two different conjugated linolenic acid (CLNA) isomers	314:367	To investigate the specific functions of conjugated fatty acids (CFAs) produced by the probiotic bacterium, α-linolenic acid was isomerized by Lactobacillus plantarum ZS2058, and two different conjugated linolenic acid (CLNA) isomers were successfully isolated: c9, t11, c15-CLNA (CLNA1) and t9, t11, c15-CLNA (CLNA2).
32125157	9	27	theme	Prevotella	1604:1613	arg1	abundance					1574:1582	the abundance	1570:1582	the abundance of Ruminococcus and Prevotella	1570:1613	CLNA1 and CLNA2 increased the abundance of Ruminococcus and Prevotella, respectively.
32125157	5	28	theme	cytokines	916:924	arg1	level					891:895	the level	887:895	the level of proinflammatory cytokines (TNF-α, IL-1β, and IL-6)	887:949	CLNA significantly attenuated the level of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) while upregulating the expression of the colonic anti-inflammatory cytokine IL-10 and nuclear receptor peroxisome-activated receptor-γ.
32125157	7	29	theme	conjugated	1307:1316	arg1	acid					1327:1330	conjugated linoleic acid	1307:1330	conjugated linoleic acid in the colonic content	1307:1353	Meanwhile, the concentrations of CLNA in the liver and conjugated linoleic acid in the colonic content were significantly increased because of the treatment of CLNA.
32125157	5	30	theme	colonic	992:998	arg1	IL-10					1027:1031	the colonic anti-inflammatory cytokine IL-10	988:1031	the colonic anti-inflammatory cytokine IL-10	988:1031	CLNA significantly attenuated the level of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) while upregulating the expression of the colonic anti-inflammatory cytokine IL-10 and nuclear receptor peroxisome-activated receptor-γ.
32125157	1	31	theme	specific	154:161	arg1	functions					163:171	the specific functions	150:171	the specific functions of conjugated fatty acids (CFAs) produced by the probiotic bacterium	150:240	To investigate the specific functions of conjugated fatty acids (CFAs) produced by the probiotic bacterium, α-linolenic acid was isomerized by Lactobacillus plantarum ZS2058, and two different conjugated linolenic acid (CLNA) isomers were successfully isolated: c9, t11, c15-CLNA (CLNA1) and t9, t11, c15-CLNA (CLNA2).
32125157	8	32	theme	intestinal	1456:1465	arg1	composition					1477:1487	the intestinal microbial composition	1452:1487	the intestinal microbial composition of colitis mice, including increasing the α-diversity	1452:1541	Furthermore, CLNA could rebalance the intestinal microbial composition of colitis mice, including increasing the α-diversity.
32125157	2	33	theme	isomers	517:523	arg1	mechanism					470:478	mechanism	470:478	mechanism	470:478	The effects and mechanism of CLNA crude extract and individual isomers on colitis were explored.
32125157	2	33	theme	isomers	517:523	arg1	effects					458:464	effects	458:464	effects	458:464	The effects and mechanism of CLNA crude extract and individual isomers on colitis were explored.
32125157	7	34	theme	linoleic	1318:1325	arg1	acid					1327:1330	conjugated linoleic acid	1307:1330	conjugated linoleic acid in the colonic content	1307:1353	Meanwhile, the concentrations of CLNA in the liver and conjugated linoleic acid in the colonic content were significantly increased because of the treatment of CLNA.
32125157	1	35	theme	α-linolenic	243:253	arg1	acid					255:258	α-linolenic acid	243:258	α-linolenic acid	243:258	To investigate the specific functions of conjugated fatty acids (CFAs) produced by the probiotic bacterium, α-linolenic acid was isomerized by Lactobacillus plantarum ZS2058, and two different conjugated linolenic acid (CLNA) isomers were successfully isolated: c9, t11, c15-CLNA (CLNA1) and t9, t11, c15-CLNA (CLNA2).
32125157	0	36	theme	Sodium	95:100	arg1	Colitis					118:124	Lactobacillus plantarum ZS2058 Ameliorate Dextran Sodium Sulfate-Induced Colitis	45:124	Lactobacillus plantarum ZS2058 Ameliorate Dextran Sodium Sulfate-Induced Colitis in Mice	45:132	c9, t11, c15-CLNA and t9, t11, c15-CLNA from Lactobacillus plantarum ZS2058 Ameliorate Dextran Sodium Sulfate-Induced Colitis in Mice.
32125157	7	37	theme	CLNA	1285:1288	arg1	Meanwhile					1252:1260	Meanwhile	1252:1260	Meanwhile	1252:1260	Meanwhile, the concentrations of CLNA in the liver and conjugated linoleic acid in the colonic content were significantly increased because of the treatment of CLNA.
32125157	7	37	theme	CLNA	1285:1288	arg1	concentrations					1267:1280	the concentrations	1263:1280	the concentrations of CLNA in the liver	1263:1301	Meanwhile, the concentrations of CLNA in the liver and conjugated linoleic acid in the colonic content were significantly increased because of the treatment of CLNA.
32125157	7	37	theme	CLNA	1285:1288	arg1	acid					1327:1330	conjugated linoleic acid	1307:1330	conjugated linoleic acid in the colonic content	1307:1353	Meanwhile, the concentrations of CLNA in the liver and conjugated linoleic acid in the colonic content were significantly increased because of the treatment of CLNA.
32125157	5	38	dep	cytokines	916:924	arg1	cytokines					916:924	proinflammatory cytokines	900:924	proinflammatory cytokines (TNF-α, IL-1β, and IL-6)	900:949	CLNA significantly attenuated the level of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) while upregulating the expression of the colonic anti-inflammatory cytokine IL-10 and nuclear receptor peroxisome-activated receptor-γ.
32125157	5	38	dep	cytokines	916:924	arg1	IL-1β					934:938	IL-1β	934:938	IL-1β	934:938	CLNA significantly attenuated the level of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) while upregulating the expression of the colonic anti-inflammatory cytokine IL-10 and nuclear receptor peroxisome-activated receptor-γ.
32125157	5	38	dep	cytokines	916:924	arg1	IL-6					945:948	IL-6	945:948	IL-6	945:948	CLNA significantly attenuated the level of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) while upregulating the expression of the colonic anti-inflammatory cytokine IL-10 and nuclear receptor peroxisome-activated receptor-γ.
32125157	5	38	dep	cytokines	916:924	arg1	TNF-α					927:931	TNF-α	927:931	TNF-α	927:931	CLNA significantly attenuated the level of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) while upregulating the expression of the colonic anti-inflammatory cytokine IL-10 and nuclear receptor peroxisome-activated receptor-γ.
32125157	0	39	theme	Dextran	87:93	arg1	Colitis					118:124	Lactobacillus plantarum ZS2058 Ameliorate Dextran Sodium Sulfate-Induced Colitis	45:124	Lactobacillus plantarum ZS2058 Ameliorate Dextran Sodium Sulfate-Induced Colitis in Mice	45:132	c9, t11, c15-CLNA and t9, t11, c15-CLNA from Lactobacillus plantarum ZS2058 Ameliorate Dextran Sodium Sulfate-Induced Colitis in Mice.
32125157	1	40	theme	conjugated	176:185	arg1	CFAs					200:203	CFAs	200:203	CFAs	200:203	To investigate the specific functions of conjugated fatty acids (CFAs) produced by the probiotic bacterium, α-linolenic acid was isomerized by Lactobacillus plantarum ZS2058, and two different conjugated linolenic acid (CLNA) isomers were successfully isolated: c9, t11, c15-CLNA (CLNA1) and t9, t11, c15-CLNA (CLNA2).
32125157	1	40	theme	conjugated	176:185	arg1	acids					193:197	conjugated fatty acids	176:197	conjugated fatty acids (CFAs) produced by the probiotic bacterium	176:240	To investigate the specific functions of conjugated fatty acids (CFAs) produced by the probiotic bacterium, α-linolenic acid was isomerized by Lactobacillus plantarum ZS2058, and two different conjugated linolenic acid (CLNA) isomers were successfully isolated: c9, t11, c15-CLNA (CLNA1) and t9, t11, c15-CLNA (CLNA2).
32125157	8	41	theme	mice	1500:1503	arg1	composition					1477:1487	the intestinal microbial composition	1452:1487	the intestinal microbial composition of colitis mice, including increasing the α-diversity	1452:1541	Furthermore, CLNA could rebalance the intestinal microbial composition of colitis mice, including increasing the α-diversity.
32125157	1	42	theme	fatty	187:191	arg1	CFAs					200:203	CFAs	200:203	CFAs	200:203	To investigate the specific functions of conjugated fatty acids (CFAs) produced by the probiotic bacterium, α-linolenic acid was isomerized by Lactobacillus plantarum ZS2058, and two different conjugated linolenic acid (CLNA) isomers were successfully isolated: c9, t11, c15-CLNA (CLNA1) and t9, t11, c15-CLNA (CLNA2).
32125157	1	42	theme	fatty	187:191	arg1	acids					193:197	conjugated fatty acids	176:197	conjugated fatty acids (CFAs) produced by the probiotic bacterium	176:240	To investigate the specific functions of conjugated fatty acids (CFAs) produced by the probiotic bacterium, α-linolenic acid was isomerized by Lactobacillus plantarum ZS2058, and two different conjugated linolenic acid (CLNA) isomers were successfully isolated: c9, t11, c15-CLNA (CLNA1) and t9, t11, c15-CLNA (CLNA2).
32125157	4	43	dep	proteins	801:808	arg1	occludin					817:824	occludin	817:824	occludin	817:824	Additionally, CLNA alleviated histological damage, protected colonic mucus layer integrity, and significantly upregulated the concentration of tight junction proteins (ZO-1, occludin, E-cadherin 1, and claudin-3).
32125157	4	43	dep	proteins	801:808	arg1	claudin-3					845:853	claudin-3	845:853	claudin-3	845:853	Additionally, CLNA alleviated histological damage, protected colonic mucus layer integrity, and significantly upregulated the concentration of tight junction proteins (ZO-1, occludin, E-cadherin 1, and claudin-3).
32125157	4	43	dep	proteins	801:808	arg1	proteins					801:808	tight junction proteins	786:808	tight junction proteins (ZO-1, occludin, E-cadherin 1, and claudin-3)	786:854	Additionally, CLNA alleviated histological damage, protected colonic mucus layer integrity, and significantly upregulated the concentration of tight junction proteins (ZO-1, occludin, E-cadherin 1, and claudin-3).
32125157	4	43	dep	proteins	801:808	arg1	ZO-1					811:814	ZO-1	811:814	ZO-1	811:814	Additionally, CLNA alleviated histological damage, protected colonic mucus layer integrity, and significantly upregulated the concentration of tight junction proteins (ZO-1, occludin, E-cadherin 1, and claudin-3).
32125157	4	43	dep	proteins	801:808	arg1	E-cadherin					827:836	E-cadherin 1	827:838	E-cadherin 1	827:838	Additionally, CLNA alleviated histological damage, protected colonic mucus layer integrity, and significantly upregulated the concentration of tight junction proteins (ZO-1, occludin, E-cadherin 1, and claudin-3).
32125157	5	44	theme	peroxisome-activated	1054:1073	arg1	receptor-γ					1075:1084	nuclear receptor peroxisome-activated receptor-γ	1037:1084	nuclear receptor peroxisome-activated receptor-γ	1037:1084	CLNA significantly attenuated the level of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) while upregulating the expression of the colonic anti-inflammatory cytokine IL-10 and nuclear receptor peroxisome-activated receptor-γ.
32125157	0	45	theme	Sulfate-Induced	102:116	arg1	Colitis					118:124	Lactobacillus plantarum ZS2058 Ameliorate Dextran Sodium Sulfate-Induced Colitis	45:124	Lactobacillus plantarum ZS2058 Ameliorate Dextran Sodium Sulfate-Induced Colitis in Mice	45:132	c9, t11, c15-CLNA and t9, t11, c15-CLNA from Lactobacillus plantarum ZS2058 Ameliorate Dextran Sodium Sulfate-Induced Colitis in Mice.
32125157	1	46	theme	acids	193:197	arg1	functions					163:171	the specific functions	150:171	the specific functions of conjugated fatty acids (CFAs) produced by the probiotic bacterium	150:240	To investigate the specific functions of conjugated fatty acids (CFAs) produced by the probiotic bacterium, α-linolenic acid was isomerized by Lactobacillus plantarum ZS2058, and two different conjugated linolenic acid (CLNA) isomers were successfully isolated: c9, t11, c15-CLNA (CLNA1) and t9, t11, c15-CLNA (CLNA2).
32125157	5	47	theme	receptor-γ	1075:1084	arg1	expression					974:983	the expression	970:983	the expression of the colonic anti-inflammatory cytokine IL-10 and nuclear receptor peroxisome-activated receptor-γ	970:1084	CLNA significantly attenuated the level of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) while upregulating the expression of the colonic anti-inflammatory cytokine IL-10 and nuclear receptor peroxisome-activated receptor-γ.
32125157	2	48	theme	CLNA	483:486	arg1	extract					494:500	CLNA crude extract	483:500	CLNA crude extract	483:500	The effects and mechanism of CLNA crude extract and individual isomers on colitis were explored.
32125157	1	49	dep	investigate	138:148	arg1	c15-CLNA					436:443	c15-CLNA	436:443	c15-CLNA (CLNA2)	436:451	To investigate the specific functions of conjugated fatty acids (CFAs) produced by the probiotic bacterium, α-linolenic acid was isomerized by Lactobacillus plantarum ZS2058, and two different conjugated linolenic acid (CLNA) isomers were successfully isolated: c9, t11, c15-CLNA (CLNA1) and t9, t11, c15-CLNA (CLNA2).
32125157	1	49	dep	investigate	138:148	arg1	c9					397:398	c9	397:398	c9	397:398	To investigate the specific functions of conjugated fatty acids (CFAs) produced by the probiotic bacterium, α-linolenic acid was isomerized by Lactobacillus plantarum ZS2058, and two different conjugated linolenic acid (CLNA) isomers were successfully isolated: c9, t11, c15-CLNA (CLNA1) and t9, t11, c15-CLNA (CLNA2).
32125157	1	49	dep	investigate	138:148	arg1	t11					401:403	t11	401:403	t11	401:403	To investigate the specific functions of conjugated fatty acids (CFAs) produced by the probiotic bacterium, α-linolenic acid was isomerized by Lactobacillus plantarum ZS2058, and two different conjugated linolenic acid (CLNA) isomers were successfully isolated: c9, t11, c15-CLNA (CLNA1) and t9, t11, c15-CLNA (CLNA2).
32125157	1	49	dep	investigate	138:148	arg1	t9					427:428	t9	427:428	t9	427:428	To investigate the specific functions of conjugated fatty acids (CFAs) produced by the probiotic bacterium, α-linolenic acid was isomerized by Lactobacillus plantarum ZS2058, and two different conjugated linolenic acid (CLNA) isomers were successfully isolated: c9, t11, c15-CLNA (CLNA1) and t9, t11, c15-CLNA (CLNA2).
32125157	1	49	dep	investigate	138:148	arg1	t11					431:433	t11	431:433	t11	431:433	To investigate the specific functions of conjugated fatty acids (CFAs) produced by the probiotic bacterium, α-linolenic acid was isomerized by Lactobacillus plantarum ZS2058, and two different conjugated linolenic acid (CLNA) isomers were successfully isolated: c9, t11, c15-CLNA (CLNA1) and t9, t11, c15-CLNA (CLNA2).
32125157	1	49	dep	investigate	138:148	arg1	c15-CLNA					406:413	c15-CLNA	406:413	c15-CLNA (CLNA1)	406:421	To investigate the specific functions of conjugated fatty acids (CFAs) produced by the probiotic bacterium, α-linolenic acid was isomerized by Lactobacillus plantarum ZS2058, and two different conjugated linolenic acid (CLNA) isomers were successfully isolated: c9, t11, c15-CLNA (CLNA1) and t9, t11, c15-CLNA (CLNA2).
32125157	1	49	dep	investigate	138:148	arg1	CLNA1					416:420	CLNA1	416:420	CLNA1	416:420	To investigate the specific functions of conjugated fatty acids (CFAs) produced by the probiotic bacterium, α-linolenic acid was isomerized by Lactobacillus plantarum ZS2058, and two different conjugated linolenic acid (CLNA) isomers were successfully isolated: c9, t11, c15-CLNA (CLNA1) and t9, t11, c15-CLNA (CLNA2).
32125157	5	50	theme	anti-inflammatory	1000:1016	arg1	IL-10					1027:1031	the colonic anti-inflammatory cytokine IL-10	988:1031	the colonic anti-inflammatory cytokine IL-10	988:1031	CLNA significantly attenuated the level of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) while upregulating the expression of the colonic anti-inflammatory cytokine IL-10 and nuclear receptor peroxisome-activated receptor-γ.
32125157	5	51	theme	receptor	1045:1052	arg1	receptor-γ					1075:1084	nuclear receptor peroxisome-activated receptor-γ	1037:1084	nuclear receptor peroxisome-activated receptor-γ	1037:1084	CLNA significantly attenuated the level of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) while upregulating the expression of the colonic anti-inflammatory cytokine IL-10 and nuclear receptor peroxisome-activated receptor-γ.
32125157	1	52	theme	Lactobacillus	278:290	arg1	ZS2058					302:307	Lactobacillus plantarum ZS2058	278:307	Lactobacillus plantarum ZS2058	278:307	To investigate the specific functions of conjugated fatty acids (CFAs) produced by the probiotic bacterium, α-linolenic acid was isomerized by Lactobacillus plantarum ZS2058, and two different conjugated linolenic acid (CLNA) isomers were successfully isolated: c9, t11, c15-CLNA (CLNA1) and t9, t11, c15-CLNA (CLNA2).
32125157	5	53	theme	IL-10	1027:1031	arg1	expression					974:983	the expression	970:983	the expression of the colonic anti-inflammatory cytokine IL-10 and nuclear receptor peroxisome-activated receptor-γ	970:1084	CLNA significantly attenuated the level of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) while upregulating the expression of the colonic anti-inflammatory cytokine IL-10 and nuclear receptor peroxisome-activated receptor-γ.
32125157	8	54	theme	colitis	1492:1498	arg1	α-diversity					1531:1541	the α-diversity	1527:1541	increasing the α-diversity	1516:1541	Furthermore, CLNA could rebalance the intestinal microbial composition of colitis mice, including increasing the α-diversity.
32125157	8	54	theme	colitis	1492:1498	arg1	mice					1500:1503	colitis mice	1492:1503	colitis mice	1492:1503	Furthermore, CLNA could rebalance the intestinal microbial composition of colitis mice, including increasing the α-diversity.
32125157	6	55	theme	enzymes	1153:1159	arg1	activity					1116:1123	the activity	1112:1123	the activity of oxidative stress-related enzymes (SOD, GSH, and CAT)	1112:1179	Moreover, CLNA increased the activity of oxidative stress-related enzymes (SOD, GSH, and CAT), and the myeloperoxidase activity was significantly decreased by CLNA.
32125157	8	56	theme	microbial	1467:1475	arg1	composition					1477:1487	the intestinal microbial composition	1452:1487	the intestinal microbial composition of colitis mice, including increasing the α-diversity	1452:1541	Furthermore, CLNA could rebalance the intestinal microbial composition of colitis mice, including increasing the α-diversity.
32125157	1	57	theme	plantarum	292:300	arg1	ZS2058					302:307	Lactobacillus plantarum ZS2058	278:307	Lactobacillus plantarum ZS2058	278:307	To investigate the specific functions of conjugated fatty acids (CFAs) produced by the probiotic bacterium, α-linolenic acid was isomerized by Lactobacillus plantarum ZS2058, and two different conjugated linolenic acid (CLNA) isomers were successfully isolated: c9, t11, c15-CLNA (CLNA1) and t9, t11, c15-CLNA (CLNA2).
32125157	6	58	theme	stress-related	1138:1151	arg1	GSH					1167:1169	GSH	1167:1169	GSH	1167:1169	Moreover, CLNA increased the activity of oxidative stress-related enzymes (SOD, GSH, and CAT), and the myeloperoxidase activity was significantly decreased by CLNA.
32125157	6	58	theme	stress-related	1138:1151	arg1	CAT					1176:1178	CAT	1176:1178	CAT	1176:1178	Moreover, CLNA increased the activity of oxidative stress-related enzymes (SOD, GSH, and CAT), and the myeloperoxidase activity was significantly decreased by CLNA.
32125157	6	58	theme	stress-related	1138:1151	arg1	SOD					1162:1164	SOD	1162:1164	SOD	1162:1164	Moreover, CLNA increased the activity of oxidative stress-related enzymes (SOD, GSH, and CAT), and the myeloperoxidase activity was significantly decreased by CLNA.
32125157	6	58	theme	stress-related	1138:1151	arg1	enzymes					1153:1159	oxidative stress-related enzymes	1128:1159	oxidative stress-related enzymes (SOD, GSH, and CAT)	1128:1179	Moreover, CLNA increased the activity of oxidative stress-related enzymes (SOD, GSH, and CAT), and the myeloperoxidase activity was significantly decreased by CLNA.
32125157	4	59	theme	colonic	704:710	arg1	integrity					724:732	colonic mucus layer integrity	704:732	colonic mucus layer integrity	704:732	Additionally, CLNA alleviated histological damage, protected colonic mucus layer integrity, and significantly upregulated the concentration of tight junction proteins (ZO-1, occludin, E-cadherin 1, and claudin-3).
32125157	0	60	from	Colitis	118:124	arg1	c15-CLNA					31:38	c15-CLNA	31:38	c15-CLNA from Lactobacillus plantarum ZS2058 Ameliorate Dextran Sodium Sulfate-Induced Colitis in Mice	31:132	c9, t11, c15-CLNA and t9, t11, c15-CLNA from Lactobacillus plantarum ZS2058 Ameliorate Dextran Sodium Sulfate-Induced Colitis in Mice.
32125157	0	60	from	Colitis	118:124	arg1	Mice					129:132	Mice	129:132	Mice	129:132	c9, t11, c15-CLNA and t9, t11, c15-CLNA from Lactobacillus plantarum ZS2058 Ameliorate Dextran Sodium Sulfate-Induced Colitis in Mice.
32125157	0	60	from	Colitis	118:124	arg1	c9					0:1	c9	0:1	c9	0:1	c9, t11, c15-CLNA and t9, t11, c15-CLNA from Lactobacillus plantarum ZS2058 Ameliorate Dextran Sodium Sulfate-Induced Colitis in Mice.
32125157	6	61	theme	myeloperoxidase	1190:1204	arg1	activity					1206:1213	the myeloperoxidase activity	1186:1213	the myeloperoxidase activity	1186:1213	Moreover, CLNA increased the activity of oxidative stress-related enzymes (SOD, GSH, and CAT), and the myeloperoxidase activity was significantly decreased by CLNA.
32125157	5	62	theme	proinflammatory	900:914	arg1	cytokines					916:924	proinflammatory cytokines	900:924	proinflammatory cytokines (TNF-α, IL-1β, and IL-6)	900:949	CLNA significantly attenuated the level of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) while upregulating the expression of the colonic anti-inflammatory cytokine IL-10 and nuclear receptor peroxisome-activated receptor-γ.
32125157	5	62	theme	proinflammatory	900:914	arg1	IL-1β					934:938	IL-1β	934:938	IL-1β	934:938	CLNA significantly attenuated the level of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) while upregulating the expression of the colonic anti-inflammatory cytokine IL-10 and nuclear receptor peroxisome-activated receptor-γ.
32125157	5	62	theme	proinflammatory	900:914	arg1	IL-6					945:948	IL-6	945:948	IL-6	945:948	CLNA significantly attenuated the level of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) while upregulating the expression of the colonic anti-inflammatory cytokine IL-10 and nuclear receptor peroxisome-activated receptor-γ.
32125157	5	62	theme	proinflammatory	900:914	arg1	TNF-α					927:931	TNF-α	927:931	TNF-α	927:931	CLNA significantly attenuated the level of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) while upregulating the expression of the colonic anti-inflammatory cytokine IL-10 and nuclear receptor peroxisome-activated receptor-γ.
32125157	8	63	theme	increasing	1516:1525	arg1	α-diversity					1531:1541	the α-diversity	1527:1541	increasing the α-diversity	1516:1541	Furthermore, CLNA could rebalance the intestinal microbial composition of colitis mice, including increasing the α-diversity.
32125157	3	64	theme	weight	580:585	arg1	loss					587:590	weight loss	580:590	weight loss	580:590	CLNA significantly inhibited weight loss, the disease activity index, and colon shortening.
32125157	4	65	theme	layer	718:722	arg1	integrity					724:732	colonic mucus layer integrity	704:732	colonic mucus layer integrity	704:732	Additionally, CLNA alleviated histological damage, protected colonic mucus layer integrity, and significantly upregulated the concentration of tight junction proteins (ZO-1, occludin, E-cadherin 1, and claudin-3).
32125157	2	66	from	effects	458:464	arg1	colitis					528:534	colitis	528:534	colitis	528:534	The effects and mechanism of CLNA crude extract and individual isomers on colitis were explored.
32125157	3	67	theme	activity	605:612	arg1	index					614:618	the disease activity index	593:618	the disease activity index	593:618	CLNA significantly inhibited weight loss, the disease activity index, and colon shortening.
32125157	4	68	theme	mucus	712:716	arg1	integrity					724:732	colonic mucus layer integrity	704:732	colonic mucus layer integrity	704:732	Additionally, CLNA alleviated histological damage, protected colonic mucus layer integrity, and significantly upregulated the concentration of tight junction proteins (ZO-1, occludin, E-cadherin 1, and claudin-3).
32125157	6	69	dep	enzymes	1153:1159	arg1	GSH					1167:1169	GSH	1167:1169	GSH	1167:1169	Moreover, CLNA increased the activity of oxidative stress-related enzymes (SOD, GSH, and CAT), and the myeloperoxidase activity was significantly decreased by CLNA.
32125157	6	69	dep	enzymes	1153:1159	arg1	CAT					1176:1178	CAT	1176:1178	CAT	1176:1178	Moreover, CLNA increased the activity of oxidative stress-related enzymes (SOD, GSH, and CAT), and the myeloperoxidase activity was significantly decreased by CLNA.
32125157	6	69	dep	enzymes	1153:1159	arg1	SOD					1162:1164	SOD	1162:1164	SOD	1162:1164	Moreover, CLNA increased the activity of oxidative stress-related enzymes (SOD, GSH, and CAT), and the myeloperoxidase activity was significantly decreased by CLNA.
32125157	6	69	dep	enzymes	1153:1159	arg1	enzymes					1153:1159	oxidative stress-related enzymes	1128:1159	oxidative stress-related enzymes (SOD, GSH, and CAT)	1128:1179	Moreover, CLNA increased the activity of oxidative stress-related enzymes (SOD, GSH, and CAT), and the myeloperoxidase activity was significantly decreased by CLNA.
32125157	7	70	from	acid	1327:1330	arg1	content					1347:1353	the colonic content	1335:1353	the colonic content	1335:1353	Meanwhile, the concentrations of CLNA in the liver and conjugated linoleic acid in the colonic content were significantly increased because of the treatment of CLNA.
32125157	7	70	from	acid	1327:1330	arg1	liver					1297:1301	the liver	1293:1301	the liver	1293:1301	Meanwhile, the concentrations of CLNA in the liver and conjugated linoleic acid in the colonic content were significantly increased because of the treatment of CLNA.
32125157	7	71	theme	CLNA	1412:1415	arg1	treatment					1399:1407	the treatment	1395:1407	the treatment of CLNA	1395:1415	Meanwhile, the concentrations of CLNA in the liver and conjugated linoleic acid in the colonic content were significantly increased because of the treatment of CLNA.
31865379	8	0	theme	biofilm	1091:1097	arg1	cultures					1099:1106	biofilm cultures	1091:1106	biofilm cultures	1091:1106	This effect is specific to biofilm cultures and is dependent upon C. albicans extracellular matrix polysaccharides, mannan and glucan, with C. albicans cells deficient in glycosylation structures not enhancing P. aeruginosa tolerance to meropenem.
31865379	9	1	theme	P.	1407:1408	arg1	aeruginosa/C					1410:1421	P. aeruginosa/C	1407:1421	P. aeruginosa/C	1407:1421	CONCLUSIONS We propose that fungal mannan and glucan secreted into the extracellular matrix of P. aeruginosa/C.
31865379	8	2	theme	P.	1274:1275	arg1	tolerance					1288:1296	P. aeruginosa tolerance	1274:1296	P. aeruginosa tolerance to meropenem	1274:1309	This effect is specific to biofilm cultures and is dependent upon C. albicans extracellular matrix polysaccharides, mannan and glucan, with C. albicans cells deficient in glycosylation structures not enhancing P. aeruginosa tolerance to meropenem.
31865379	7	3	theme	C.	946:947	arg1	RESULTS					938:944	RESULTS	938:944	RESULTS C. albicans	938:956	RESULTS C. albicans enhances P. aeruginosa biofilm tolerance to meropenem at the clinically relevant concentration of 5 mg/L.
31865379	7	4	dep	P.	967:968	arg1	aeruginosa					970:979	aeruginosa	970:979	aeruginosa	970:979	RESULTS C. albicans enhances P. aeruginosa biofilm tolerance to meropenem at the clinically relevant concentration of 5 mg/L.
31865379	0	5	theme	dual-species	77:88	arg1	biofilm					90:96	a dual-species biofilm	75:96	a dual-species biofilm	75:96	Candida albicans enhances meropenem tolerance of Pseudomonas aeruginosa in a dual-species biofilm.
31865379	8	6	from	deficient	1222:1230	arg1	structures					1249:1258	glycosylation structures	1235:1258	glycosylation structures not enhancing P. aeruginosa tolerance to meropenem	1235:1309	This effect is specific to biofilm cultures and is dependent upon C. albicans extracellular matrix polysaccharides, mannan and glucan, with C. albicans cells deficient in glycosylation structures not enhancing P. aeruginosa tolerance to meropenem.
31865379	10	7	theme	P.	1488:1489	arg1	tolerance					1502:1510	P. aeruginosa tolerance	1488:1510	P. aeruginosa tolerance to meropenem, which has direct implications for the treatment of coinfected patients	1488:1595	albicans dual-species biofilms play a central role in enhancing P. aeruginosa tolerance to meropenem, which has direct implications for the treatment of coinfected patients.
31865379	3	8	theme	medical	508:514	arg1	devices					516:522	medical devices	508:522	medical devices	508:522	Both P. aeruginosa and C. albicans are able to form extensive biofilms on the surfaces of mucosa and medical devices.
31865379	10	9	dep	P.	1488:1489	arg1	aeruginosa					1491:1500	aeruginosa	1491:1500	aeruginosa	1491:1500	albicans dual-species biofilms play a central role in enhancing P. aeruginosa tolerance to meropenem, which has direct implications for the treatment of coinfected patients.
31865379	1	10	theme	indwelling	259:268	arg1	devices					278:284	indwelling medical devices	259:284	indwelling medical devices	259:284	BACKGROUND Pseudomonas aeruginosa is an opportunistic bacterium that infects the airways of cystic fibrosis patients, surfaces of surgical and burn wounds, and indwelling medical devices.
31865379	8	11	theme	deficient	1222:1230	arg1	cells					1216:1220	C. albicans cells	1204:1220	C. albicans cells deficient in glycosylation structures not enhancing P. aeruginosa tolerance to meropenem	1204:1309	This effect is specific to biofilm cultures and is dependent upon C. albicans extracellular matrix polysaccharides, mannan and glucan, with C. albicans cells deficient in glycosylation structures not enhancing P. aeruginosa tolerance to meropenem.
31865379	8	12	from	structures	1249:1258	arg1	deficient					1222:1230	deficient	1222:1230	deficient	1222:1230	This effect is specific to biofilm cultures and is dependent upon C. albicans extracellular matrix polysaccharides, mannan and glucan, with C. albicans cells deficient in glycosylation structures not enhancing P. aeruginosa tolerance to meropenem.
31865379	6	13	theme	electron	852:859	arg1	microscopy					861:870	Scanning electron microscopy	843:870	Scanning electron microscopy	843:870	Scanning electron microscopy and confocal microscopy were used to visualize biofilm structure.
31865379	1	14	theme	medical	270:276	arg1	devices					278:284	indwelling medical devices	259:284	indwelling medical devices	259:284	BACKGROUND Pseudomonas aeruginosa is an opportunistic bacterium that infects the airways of cystic fibrosis patients, surfaces of surgical and burn wounds, and indwelling medical devices.
31865379	10	15	theme	central	1462:1468	arg1	role					1470:1473	a central role	1460:1473	a central role	1460:1473	albicans dual-species biofilms play a central role in enhancing P. aeruginosa tolerance to meropenem, which has direct implications for the treatment of coinfected patients.
31865379	9	16	theme	extracellular	1383:1395	arg1	matrix					1397:1402	the extracellular matrix	1379:1402	the extracellular matrix of P. aeruginosa/C	1379:1421	CONCLUSIONS We propose that fungal mannan and glucan secreted into the extracellular matrix of P. aeruginosa/C.
31865379	5	17	theme	dual-species	679:690	arg1	biofilms					692:699	dual-species biofilms	679:699	dual-species biofilms	679:699	METHODS Single- and dual-species biofilms were established in microtitre plates and the survival of each species was measured following treatment with clinically relevant antibiotics.
31865379	5	17	theme	dual-species	679:690	arg1	METHODS					659:665	METHODS Single- and dual-species biofilms	659:699	METHODS Single- and dual-species biofilms	659:699	METHODS Single- and dual-species biofilms were established in microtitre plates and the survival of each species was measured following treatment with clinically relevant antibiotics.
31865379	6	18	theme	Scanning	843:850	arg1	microscopy					861:870	Scanning electron microscopy	843:870	Scanning electron microscopy	843:870	Scanning electron microscopy and confocal microscopy were used to visualize biofilm structure.
31865379	4	19	theme	antibiotic	594:603	arg1	tolerance					605:613	antibiotic tolerance	594:613	antibiotic tolerance of P. aeruginosa	594:630	OBJECTIVES To determine whether the presence of C. albicans enhances antibiotic tolerance of P. aeruginosa in a dual-species biofilm.
31865379	8	20	theme	glycosylation	1235:1247	arg1	structures					1249:1258	glycosylation structures	1235:1258	glycosylation structures not enhancing P. aeruginosa tolerance to meropenem	1235:1309	This effect is specific to biofilm cultures and is dependent upon C. albicans extracellular matrix polysaccharides, mannan and glucan, with C. albicans cells deficient in glycosylation structures not enhancing P. aeruginosa tolerance to meropenem.
31865379	10	21	contain	has	1532:1534	arg2	implications					1543:1554	direct implications	1536:1554	direct implications for the treatment of coinfected patients	1536:1595	albicans dual-species biofilms play a central role in enhancing P. aeruginosa tolerance to meropenem, which has direct implications for the treatment of coinfected patients.
31865379	10	21	contain	has	1532:1534	arg1	meropenem					1515:1523	meropenem	1515:1523	meropenem	1515:1523	albicans dual-species biofilms play a central role in enhancing P. aeruginosa tolerance to meropenem, which has direct implications for the treatment of coinfected patients.
31865379	5	22	theme	species	764:770	arg1	survival					747:754	the survival	743:754	the survival of each species	743:770	METHODS Single- and dual-species biofilms were established in microtitre plates and the survival of each species was measured following treatment with clinically relevant antibiotics.
31865379	8	23	dep	C.	1204:1205	arg1	albicans					1207:1214	albicans	1207:1214	albicans	1207:1214	This effect is specific to biofilm cultures and is dependent upon C. albicans extracellular matrix polysaccharides, mannan and glucan, with C. albicans cells deficient in glycosylation structures not enhancing P. aeruginosa tolerance to meropenem.
31865379	1	24	theme	cystic	191:196	arg1	fibrosis					198:205	cystic fibrosis	191:205	cystic fibrosis patients	191:214	BACKGROUND Pseudomonas aeruginosa is an opportunistic bacterium that infects the airways of cystic fibrosis patients, surfaces of surgical and burn wounds, and indwelling medical devices.
31865379	8	25	theme	C.	1204:1205	arg1	cells					1216:1220	C. albicans cells	1204:1220	C. albicans cells deficient in glycosylation structures not enhancing P. aeruginosa tolerance to meropenem	1204:1309	This effect is specific to biofilm cultures and is dependent upon C. albicans extracellular matrix polysaccharides, mannan and glucan, with C. albicans cells deficient in glycosylation structures not enhancing P. aeruginosa tolerance to meropenem.
31865379	8	26	theme	C.	1130:1131	arg1	mannan					1180:1185	mannan	1180:1185	mannan	1180:1185	This effect is specific to biofilm cultures and is dependent upon C. albicans extracellular matrix polysaccharides, mannan and glucan, with C. albicans cells deficient in glycosylation structures not enhancing P. aeruginosa tolerance to meropenem.
31865379	8	26	theme	C.	1130:1131	arg1	polysaccharides					1163:1177	C. albicans extracellular matrix polysaccharides	1130:1177	C. albicans extracellular matrix polysaccharides	1130:1177	This effect is specific to biofilm cultures and is dependent upon C. albicans extracellular matrix polysaccharides, mannan and glucan, with C. albicans cells deficient in glycosylation structures not enhancing P. aeruginosa tolerance to meropenem.
31865379	8	26	theme	C.	1130:1131	arg1	glucan					1191:1196	glucan	1191:1196	glucan	1191:1196	This effect is specific to biofilm cultures and is dependent upon C. albicans extracellular matrix polysaccharides, mannan and glucan, with C. albicans cells deficient in glycosylation structures not enhancing P. aeruginosa tolerance to meropenem.
31865379	1	27	theme	fibrosis	198:205	arg1	patients					207:214	cystic fibrosis patients	191:214	cystic fibrosis patients	191:214	BACKGROUND Pseudomonas aeruginosa is an opportunistic bacterium that infects the airways of cystic fibrosis patients, surfaces of surgical and burn wounds, and indwelling medical devices.
31865379	0	28	theme	Candida	0:6	arg1	albicans					8:15	Candida albicans	0:15	Candida albicans	0:15	Candida albicans enhances meropenem tolerance of Pseudomonas aeruginosa in a dual-species biofilm.
31865379	9	29	theme	aeruginosa/C	1410:1421	arg1	matrix					1397:1402	the extracellular matrix	1379:1402	the extracellular matrix of P. aeruginosa/C	1379:1421	CONCLUSIONS We propose that fungal mannan and glucan secreted into the extracellular matrix of P. aeruginosa/C.
31865379	7	30	theme	biofilm	981:987	arg1	tolerance					989:997	P. aeruginosa biofilm tolerance	967:997	P. aeruginosa biofilm tolerance to meropenem	967:1010	RESULTS C. albicans enhances P. aeruginosa biofilm tolerance to meropenem at the clinically relevant concentration of 5 mg/L.
31865379	2	31	theme	fungal	319:324	arg1	infections					326:335	secondary fungal infections	309:335	secondary fungal infections	309:335	Patients are prone to secondary fungal infections, with Candida albicans being commonly co-isolated with P. aeruginosa.
31865379	1	32	theme	patients	207:214	arg1	surfaces					217:224	surfaces	217:224	surfaces of surgical and burn wounds	217:252	BACKGROUND Pseudomonas aeruginosa is an opportunistic bacterium that infects the airways of cystic fibrosis patients, surfaces of surgical and burn wounds, and indwelling medical devices.
31865379	1	32	theme	patients	207:214	arg1	devices					278:284	indwelling medical devices	259:284	indwelling medical devices	259:284	BACKGROUND Pseudomonas aeruginosa is an opportunistic bacterium that infects the airways of cystic fibrosis patients, surfaces of surgical and burn wounds, and indwelling medical devices.
31865379	1	32	theme	patients	207:214	arg1	airways					180:186	the airways	176:186	the airways of cystic fibrosis patients	176:214	BACKGROUND Pseudomonas aeruginosa is an opportunistic bacterium that infects the airways of cystic fibrosis patients, surfaces of surgical and burn wounds, and indwelling medical devices.
31865379	0	33	theme	meropenem	26:34	arg1	tolerance					36:44	meropenem tolerance	26:44	meropenem tolerance of Pseudomonas aeruginosa	26:70	Candida albicans enhances meropenem tolerance of Pseudomonas aeruginosa in a dual-species biofilm.
31865379	5	34	theme	microtitre	721:730	arg1	plates					732:737	microtitre plates	721:737	microtitre plates	721:737	METHODS Single- and dual-species biofilms were established in microtitre plates and the survival of each species was measured following treatment with clinically relevant antibiotics.
31865379	7	35	dep	C.	946:947	arg1	albicans					949:956	albicans	949:956	albicans	949:956	RESULTS C. albicans enhances P. aeruginosa biofilm tolerance to meropenem at the clinically relevant concentration of 5 mg/L.
31865379	2	36	theme	secondary	309:317	arg1	infections					326:335	secondary fungal infections	309:335	secondary fungal infections	309:335	Patients are prone to secondary fungal infections, with Candida albicans being commonly co-isolated with P. aeruginosa.
31865379	4	37	theme	dual-species	637:648	arg1	biofilm					650:656	a dual-species biofilm	635:656	a dual-species biofilm	635:656	OBJECTIVES To determine whether the presence of C. albicans enhances antibiotic tolerance of P. aeruginosa in a dual-species biofilm.
31865379	4	38	theme	C.	573:574	arg1	albicans					576:583	C. albicans	573:583	C. albicans	573:583	OBJECTIVES To determine whether the presence of C. albicans enhances antibiotic tolerance of P. aeruginosa in a dual-species biofilm.
31865379	10	39	theme	patients	1588:1595	arg1	treatment					1564:1572	the treatment	1560:1572	the treatment of coinfected patients	1560:1595	albicans dual-species biofilms play a central role in enhancing P. aeruginosa tolerance to meropenem, which has direct implications for the treatment of coinfected patients.
31865379	6	40	theme	biofilm	919:925	arg1	structure					927:935	biofilm structure	919:935	biofilm structure	919:935	Scanning electron microscopy and confocal microscopy were used to visualize biofilm structure.
31865379	1	41	dep	BACKGROUND	99:108	arg1	aeruginosa					122:131	Pseudomonas aeruginosa	110:131	BACKGROUND Pseudomonas aeruginosa	99:131	BACKGROUND Pseudomonas aeruginosa is an opportunistic bacterium that infects the airways of cystic fibrosis patients, surfaces of surgical and burn wounds, and indwelling medical devices.
31865379	6	42	used	used	901:904	arg2	microscopy					861:870	Scanning electron microscopy	843:870	Scanning electron microscopy	843:870	Scanning electron microscopy and confocal microscopy were used to visualize biofilm structure.
31865379	6	42	used	used	901:904	arg2	microscopy					885:894	confocal microscopy	876:894	confocal microscopy	876:894	Scanning electron microscopy and confocal microscopy were used to visualize biofilm structure.
31865379	3	43	theme	mucosa	497:502	arg1	surfaces					485:492	the surfaces	481:492	the surfaces of mucosa and medical devices	481:522	Both P. aeruginosa and C. albicans are able to form extensive biofilms on the surfaces of mucosa and medical devices.
31865379	10	44	theme	coinfected	1577:1586	arg1	patients					1588:1595	coinfected patients	1577:1595	coinfected patients	1577:1595	albicans dual-species biofilms play a central role in enhancing P. aeruginosa tolerance to meropenem, which has direct implications for the treatment of coinfected patients.
31865379	8	45	with	polysaccharides	1163:1177	arg1	cells					1216:1220	C. albicans cells	1204:1220	C. albicans cells deficient in glycosylation structures not enhancing P. aeruginosa tolerance to meropenem	1204:1309	This effect is specific to biofilm cultures and is dependent upon C. albicans extracellular matrix polysaccharides, mannan and glucan, with C. albicans cells deficient in glycosylation structures not enhancing P. aeruginosa tolerance to meropenem.
31865379	4	46	theme	albicans	576:583	arg1	presence					561:568	the presence	557:568	the presence of C. albicans	557:583	OBJECTIVES To determine whether the presence of C. albicans enhances antibiotic tolerance of P. aeruginosa in a dual-species biofilm.
31865379	5	47	dep	METHODS	659:665	arg1	Single-					667:673	Single-	667:673	Single-	667:673	METHODS Single- and dual-species biofilms were established in microtitre plates and the survival of each species was measured following treatment with clinically relevant antibiotics.
31865379	5	47	dep	METHODS	659:665	arg1	biofilms					692:699	dual-species biofilms	679:699	dual-species biofilms	679:699	METHODS Single- and dual-species biofilms were established in microtitre plates and the survival of each species was measured following treatment with clinically relevant antibiotics.
31865379	5	47	dep	METHODS	659:665	arg1	METHODS					659:665	METHODS Single- and dual-species biofilms	659:699	METHODS Single- and dual-species biofilms	659:699	METHODS Single- and dual-species biofilms were established in microtitre plates and the survival of each species was measured following treatment with clinically relevant antibiotics.
31865379	10	48	theme	dual-species	1433:1444	arg1	biofilms					1446:1453	albicans dual-species biofilms	1424:1453	albicans dual-species biofilms	1424:1453	albicans dual-species biofilms play a central role in enhancing P. aeruginosa tolerance to meropenem, which has direct implications for the treatment of coinfected patients.
31865379	3	49	theme	extensive	459:467	arg1	biofilms					469:476	extensive biofilms	459:476	extensive biofilms	459:476	Both P. aeruginosa and C. albicans are able to form extensive biofilms on the surfaces of mucosa and medical devices.
31865379	0	50	theme	aeruginosa	61:70	arg1	tolerance					36:44	meropenem tolerance	26:44	meropenem tolerance of Pseudomonas aeruginosa	26:70	Candida albicans enhances meropenem tolerance of Pseudomonas aeruginosa in a dual-species biofilm.
31865379	8	51	dep	P.	1274:1275	arg1	aeruginosa					1277:1286	aeruginosa	1277:1286	aeruginosa	1277:1286	This effect is specific to biofilm cultures and is dependent upon C. albicans extracellular matrix polysaccharides, mannan and glucan, with C. albicans cells deficient in glycosylation structures not enhancing P. aeruginosa tolerance to meropenem.
31865379	10	52	theme	albicans	1424:1431	arg1	biofilms					1446:1453	albicans dual-species biofilms	1424:1453	albicans dual-species biofilms	1424:1453	albicans dual-species biofilms play a central role in enhancing P. aeruginosa tolerance to meropenem, which has direct implications for the treatment of coinfected patients.
31865379	8	53	theme	matrix	1156:1161	arg1	mannan					1180:1185	mannan	1180:1185	mannan	1180:1185	This effect is specific to biofilm cultures and is dependent upon C. albicans extracellular matrix polysaccharides, mannan and glucan, with C. albicans cells deficient in glycosylation structures not enhancing P. aeruginosa tolerance to meropenem.
31865379	8	53	theme	matrix	1156:1161	arg1	polysaccharides					1163:1177	C. albicans extracellular matrix polysaccharides	1130:1177	C. albicans extracellular matrix polysaccharides	1130:1177	This effect is specific to biofilm cultures and is dependent upon C. albicans extracellular matrix polysaccharides, mannan and glucan, with C. albicans cells deficient in glycosylation structures not enhancing P. aeruginosa tolerance to meropenem.
31865379	8	53	theme	matrix	1156:1161	arg1	glucan					1191:1196	glucan	1191:1196	glucan	1191:1196	This effect is specific to biofilm cultures and is dependent upon C. albicans extracellular matrix polysaccharides, mannan and glucan, with C. albicans cells deficient in glycosylation structures not enhancing P. aeruginosa tolerance to meropenem.
31865379	8	54	theme	extracellular	1142:1154	arg1	mannan					1180:1185	mannan	1180:1185	mannan	1180:1185	This effect is specific to biofilm cultures and is dependent upon C. albicans extracellular matrix polysaccharides, mannan and glucan, with C. albicans cells deficient in glycosylation structures not enhancing P. aeruginosa tolerance to meropenem.
31865379	8	54	theme	extracellular	1142:1154	arg1	polysaccharides					1163:1177	C. albicans extracellular matrix polysaccharides	1130:1177	C. albicans extracellular matrix polysaccharides	1130:1177	This effect is specific to biofilm cultures and is dependent upon C. albicans extracellular matrix polysaccharides, mannan and glucan, with C. albicans cells deficient in glycosylation structures not enhancing P. aeruginosa tolerance to meropenem.
31865379	8	54	theme	extracellular	1142:1154	arg1	glucan					1191:1196	glucan	1191:1196	glucan	1191:1196	This effect is specific to biofilm cultures and is dependent upon C. albicans extracellular matrix polysaccharides, mannan and glucan, with C. albicans cells deficient in glycosylation structures not enhancing P. aeruginosa tolerance to meropenem.
31865379	2	55	theme	Candida	343:349	arg1	albicans					351:358	Candida albicans	343:358	Candida albicans	343:358	Patients are prone to secondary fungal infections, with Candida albicans being commonly co-isolated with P. aeruginosa.
31865379	7	56	theme	P.	967:968	arg1	tolerance					989:997	P. aeruginosa biofilm tolerance	967:997	P. aeruginosa biofilm tolerance to meropenem	967:1010	RESULTS C. albicans enhances P. aeruginosa biofilm tolerance to meropenem at the clinically relevant concentration of 5 mg/L.
31865379	9	57	theme	fungal	1340:1345	arg1	mannan					1347:1352	mannan	1347:1352	mannan	1347:1352	CONCLUSIONS We propose that fungal mannan and glucan secreted into the extracellular matrix of P. aeruginosa/C.
31865379	7	58	theme	relevant	1030:1037	arg1	concentration					1039:1051	the clinically relevant concentration	1015:1051	the clinically relevant concentration of 5 mg/L	1015:1061	RESULTS C. albicans enhances P. aeruginosa biofilm tolerance to meropenem at the clinically relevant concentration of 5 mg/L.
31865379	1	59	theme	opportunistic	139:151	arg1	BACKGROUND					99:108	BACKGROUND	99:108	BACKGROUND Pseudomonas aeruginosa	99:131	BACKGROUND Pseudomonas aeruginosa is an opportunistic bacterium that infects the airways of cystic fibrosis patients, surfaces of surgical and burn wounds, and indwelling medical devices.
31865379	1	59	theme	opportunistic	139:151	arg1	bacterium					153:161	an opportunistic bacterium	136:161	an opportunistic bacterium that infects the airways of cystic fibrosis patients, surfaces of surgical and burn wounds, and indwelling medical devices	136:284	BACKGROUND Pseudomonas aeruginosa is an opportunistic bacterium that infects the airways of cystic fibrosis patients, surfaces of surgical and burn wounds, and indwelling medical devices.
31865379	6	60	theme	confocal	876:883	arg1	microscopy					885:894	confocal microscopy	876:894	confocal microscopy	876:894	Scanning electron microscopy and confocal microscopy were used to visualize biofilm structure.
31865379	5	61	with	treatment	795:803	arg1	antibiotics					830:840	clinically relevant antibiotics	810:840	clinically relevant antibiotics	810:840	METHODS Single- and dual-species biofilms were established in microtitre plates and the survival of each species was measured following treatment with clinically relevant antibiotics.
31865379	7	62	theme	5 mg/L	1056:1061	arg1	concentration					1039:1051	the clinically relevant concentration	1015:1051	the clinically relevant concentration of 5 mg/L	1015:1061	RESULTS C. albicans enhances P. aeruginosa biofilm tolerance to meropenem at the clinically relevant concentration of 5 mg/L.
31865379	1	63	theme	burn	242:245	arg1	wounds					247:252	surgical and burn wounds	229:252	surgical and burn wounds	229:252	BACKGROUND Pseudomonas aeruginosa is an opportunistic bacterium that infects the airways of cystic fibrosis patients, surfaces of surgical and burn wounds, and indwelling medical devices.
31865379	8	64	dep	C.	1130:1131	arg1	albicans					1133:1140	albicans	1133:1140	albicans	1133:1140	This effect is specific to biofilm cultures and is dependent upon C. albicans extracellular matrix polysaccharides, mannan and glucan, with C. albicans cells deficient in glycosylation structures not enhancing P. aeruginosa tolerance to meropenem.
31865379	1	65	theme	surgical	229:236	arg1	wounds					247:252	surgical and burn wounds	229:252	surgical and burn wounds	229:252	BACKGROUND Pseudomonas aeruginosa is an opportunistic bacterium that infects the airways of cystic fibrosis patients, surfaces of surgical and burn wounds, and indwelling medical devices.
31865379	3	66	theme	devices	516:522	arg1	surfaces					485:492	the surfaces	481:492	the surfaces of mucosa and medical devices	481:522	Both P. aeruginosa and C. albicans are able to form extensive biofilms on the surfaces of mucosa and medical devices.
31865379	1	67	theme	wounds	247:252	arg1	surfaces					217:224	surfaces	217:224	surfaces of surgical and burn wounds	217:252	BACKGROUND Pseudomonas aeruginosa is an opportunistic bacterium that infects the airways of cystic fibrosis patients, surfaces of surgical and burn wounds, and indwelling medical devices.
31865379	1	67	theme	wounds	247:252	arg1	devices					278:284	indwelling medical devices	259:284	indwelling medical devices	259:284	BACKGROUND Pseudomonas aeruginosa is an opportunistic bacterium that infects the airways of cystic fibrosis patients, surfaces of surgical and burn wounds, and indwelling medical devices.
31865379	1	67	theme	wounds	247:252	arg1	airways					180:186	the airways	176:186	the airways of cystic fibrosis patients	176:214	BACKGROUND Pseudomonas aeruginosa is an opportunistic bacterium that infects the airways of cystic fibrosis patients, surfaces of surgical and burn wounds, and indwelling medical devices.
31865379	5	68	theme	relevant	821:828	arg1	antibiotics					830:840	clinically relevant antibiotics	810:840	clinically relevant antibiotics	810:840	METHODS Single- and dual-species biofilms were established in microtitre plates and the survival of each species was measured following treatment with clinically relevant antibiotics.
31865379	9	69	dep	CONCLUSIONS	1312:1322	arg1	propose					1327:1333	propose	1327:1333	propose that fungal mannan and glucan secreted into the extracellular matrix of P. aeruginosa/C	1327:1421	CONCLUSIONS We propose that fungal mannan and glucan secreted into the extracellular matrix of P. aeruginosa/C.
31865379	10	70	theme	direct	1536:1541	arg1	implications					1543:1554	direct implications	1536:1554	direct implications for the treatment of coinfected patients	1536:1595	albicans dual-species biofilms play a central role in enhancing P. aeruginosa tolerance to meropenem, which has direct implications for the treatment of coinfected patients.
31865379	4	71	theme	aeruginosa	621:630	arg1	tolerance					605:613	antibiotic tolerance	594:613	antibiotic tolerance of P. aeruginosa	594:630	OBJECTIVES To determine whether the presence of C. albicans enhances antibiotic tolerance of P. aeruginosa in a dual-species biofilm.
33806952	10	0	theme	%	1742:1742	arg1	humidity					1753:1760	75% relative humidity	1740:1760	75% relative humidity	1740:1760	The solid NSSPE was stable for 3 months under the accelerated condition of 75% relative humidity and 40 °C. Thus, it is possible todevelop the solid NSSPE preserving the unique microstructure and the superior properties in vitro of the liquid NSSPE for poorly soluble drugs.
33806952	1	1	theme	self-stabilized	154:168	arg1	NSSPE					190:194	NSSPE	190:194	NSSPE	190:194	A drug nanocrystals self-stabilized Pickering emulsion (NSSPE) with a unique composition and microstructure has been proven to significantly increase the bioavailability of poorly soluble drugs.
33806952	1	1	theme	self-stabilized	154:168	arg1	emulsion					180:187	A drug nanocrystals self-stabilized Pickering emulsion	134:187	A drug nanocrystals self-stabilized Pickering emulsion (NSSPE) with a unique composition and microstructure	134:240	A drug nanocrystals self-stabilized Pickering emulsion (NSSPE) with a unique composition and microstructure has been proven to significantly increase the bioavailability of poorly soluble drugs.
33806952	4	2	theme	optimized	626:634	arg1	NSSPE					642:646	the optimized solid NSSPE	622:646	the optimized solid NSSPE	622:646	The drug release and stability of the optimized solid NSSPE in vitro were also investigated.
33806952	0	3	with	Spray-Drying	74:85	arg1	Hydroxypropyl-β-cyclodextrin					92:119	Hydroxypropyl-β-cyclodextrin	92:119	Hydroxypropyl-β-cyclodextrin as Carriers	92:131	A Novel Solid Nanocrystals Self-Stabilized Pickering Emulsion Prepared by Spray-Drying with Hydroxypropyl-β-cyclodextrin as Carriers.
33806952	10	4	theme	relative	1744:1751	arg1	humidity					1753:1760	75% relative humidity	1740:1760	75% relative humidity	1740:1760	The solid NSSPE was stable for 3 months under the accelerated condition of 75% relative humidity and 40 °C. Thus, it is possible todevelop the solid NSSPE preserving the unique microstructure and the superior properties in vitro of the liquid NSSPE for poorly soluble drugs.
33806952	9	5	theme	cumulative	1468:1477	arg1	amount					1479:1484	The cumulative amount	1464:1484	The cumulative amount of released puerarin from the solid NSSPE	1464:1526	The cumulative amount of released puerarin from the solid NSSPE had no significant difference compared with the liquid NSSPE, which were both significantly higher than that of puerarin crude material.
33806952	9	5	theme	cumulative	1468:1477	arg1	puerarin					1498:1505	released puerarin	1489:1505	released puerarin from the solid NSSPE	1489:1526	The cumulative amount of released puerarin from the solid NSSPE had no significant difference compared with the liquid NSSPE, which were both significantly higher than that of puerarin crude material.
33806952	7	6	theme	electron	1143:1150	arg1	microscopy					1152:1161	scanning electron microscopy	1134:1161	scanning electron microscopy	1134:1161	Fluorescence microscopy, confocal laser scanning microscopy and scanning electron microscopy showed that the reconstituted emulsion of the solid NSSPE prepared with HP-β-CD had the same core-shell structure with a core of oil and a shell of puerarin nanocrystals as the liquid NSSPE.
33806952	10	7	theme	possible	1785:1792	arg1	todevelop					1794:1802	possible todevelop	1785:1802	possible todevelop the solid NSSPE preserving the unique microstructure and the superior properties in vitro of the liquid NSSPE for poorly soluble drugs	1785:1937	The solid NSSPE was stable for 3 months under the accelerated condition of 75% relative humidity and 40 °C. Thus, it is possible todevelop the solid NSSPE preserving the unique microstructure and the superior properties in vitro of the liquid NSSPE for poorly soluble drugs.
33806952	9	8	theme	puerarin	1498:1505	arg1	amount					1479:1484	The cumulative amount	1464:1484	The cumulative amount of released puerarin from the solid NSSPE	1464:1526	The cumulative amount of released puerarin from the solid NSSPE had no significant difference compared with the liquid NSSPE, which were both significantly higher than that of puerarin crude material.
33806952	9	8	theme	puerarin	1498:1505	arg1	puerarin					1498:1505	released puerarin	1489:1505	released puerarin from the solid NSSPE	1489:1526	The cumulative amount of released puerarin from the solid NSSPE had no significant difference compared with the liquid NSSPE, which were both significantly higher than that of puerarin crude material.
33806952	6	9	theme	solid	1008:1012	arg1	NSSPE					1014:1018	the solid NSSPE	1004:1018	the solid NSSPE than the feed rate and the inlet air temperature	1004:1067	The amount of HP-β-CD had more influences on the solid NSSPE than the feed rate and the inlet air temperature.
33806952	7	10	theme	scanning	1110:1117	arg1	microscopy					1119:1128	confocal laser scanning microscopy	1095:1128	confocal laser scanning microscopy	1095:1128	Fluorescence microscopy, confocal laser scanning microscopy and scanning electron microscopy showed that the reconstituted emulsion of the solid NSSPE prepared with HP-β-CD had the same core-shell structure with a core of oil and a shell of puerarin nanocrystals as the liquid NSSPE.
33806952	5	11	theme	solid	757:761	arg1	carriers					763:770	solid carriers	757:770	solid carriers commonly used in solidification of traditional Pickering emulsions	757:837	The results showed that hydroxypropyl-β-cyclodextrin (HP-β-CD), rather than solid carriers commonly used in solidification of traditional Pickering emulsions, was suitable for the solid NSSPE to retain the original appearance and size of emulsion droplets after reconstitution.
33806952	0	12	theme	Nanocrystals	14:25	arg1	Emulsion					53:60	A Novel Solid Nanocrystals Self-Stabilized Pickering Emulsion	0:60	A Novel Solid Nanocrystals Self-Stabilized Pickering Emulsion	0:60	A Novel Solid Nanocrystals Self-Stabilized Pickering Emulsion Prepared by Spray-Drying with Hydroxypropyl-β-cyclodextrin as Carriers.
33806952	5	13	theme	solid	861:865	arg1	NSSPE					867:871	the solid NSSPE	857:871	the solid NSSPE	857:871	The results showed that hydroxypropyl-β-cyclodextrin (HP-β-CD), rather than solid carriers commonly used in solidification of traditional Pickering emulsions, was suitable for the solid NSSPE to retain the original appearance and size of emulsion droplets after reconstitution.
33806952	6	14	theme	more	985:988	arg1	influences					990:999	more influences	985:999	more influences	985:999	The amount of HP-β-CD had more influences on the solid NSSPE than the feed rate and the inlet air temperature.
33806952	1	15	theme	nanocrystals	141:152	arg1	NSSPE					190:194	NSSPE	190:194	NSSPE	190:194	A drug nanocrystals self-stabilized Pickering emulsion (NSSPE) with a unique composition and microstructure has been proven to significantly increase the bioavailability of poorly soluble drugs.
33806952	1	15	theme	nanocrystals	141:152	arg1	emulsion					180:187	A drug nanocrystals self-stabilized Pickering emulsion	134:187	A drug nanocrystals self-stabilized Pickering emulsion (NSSPE) with a unique composition and microstructure	134:240	A drug nanocrystals self-stabilized Pickering emulsion (NSSPE) with a unique composition and microstructure has been proven to significantly increase the bioavailability of poorly soluble drugs.
33806952	6	16	theme	HP-β-CD	973:979	arg1	HP-β-CD					973:979	HP-β-CD	973:979	HP-β-CD	973:979	The amount of HP-β-CD had more influences on the solid NSSPE than the feed rate and the inlet air temperature.
33806952	6	16	theme	HP-β-CD	973:979	arg1	amount					963:968	The amount	959:968	The amount of HP-β-CD	959:979	The amount of HP-β-CD had more influences on the solid NSSPE than the feed rate and the inlet air temperature.
33806952	10	17	dep	todevelop	1794:1802	arg1	preserving					1820:1829	preserving	1820:1829	preserving the unique microstructure and the superior properties in vitro of the liquid NSSPE for poorly soluble drugs	1820:1937	The solid NSSPE was stable for 3 months under the accelerated condition of 75% relative humidity and 40 °C. Thus, it is possible todevelop the solid NSSPE preserving the unique microstructure and the superior properties in vitro of the liquid NSSPE for poorly soluble drugs.
33806952	1	18	theme	Pickering	170:178	arg1	NSSPE					190:194	NSSPE	190:194	NSSPE	190:194	A drug nanocrystals self-stabilized Pickering emulsion (NSSPE) with a unique composition and microstructure has been proven to significantly increase the bioavailability of poorly soluble drugs.
33806952	1	18	theme	Pickering	170:178	arg1	emulsion					180:187	A drug nanocrystals self-stabilized Pickering emulsion	134:187	A drug nanocrystals self-stabilized Pickering emulsion (NSSPE) with a unique composition and microstructure	134:240	A drug nanocrystals self-stabilized Pickering emulsion (NSSPE) with a unique composition and microstructure has been proven to significantly increase the bioavailability of poorly soluble drugs.
33806952	6	19	theme	inlet	1047:1051	arg1	temperature					1057:1067	the inlet air temperature	1043:1067	the inlet air temperature	1043:1067	The amount of HP-β-CD had more influences on the solid NSSPE than the feed rate and the inlet air temperature.
33806952	7	20	theme	NSSPE	1215:1219	arg1	emulsion					1193:1200	the reconstituted emulsion	1175:1200	the reconstituted emulsion of the solid NSSPE prepared with HP-β-CD	1175:1241	Fluorescence microscopy, confocal laser scanning microscopy and scanning electron microscopy showed that the reconstituted emulsion of the solid NSSPE prepared with HP-β-CD had the same core-shell structure with a core of oil and a shell of puerarin nanocrystals as the liquid NSSPE.
33806952	5	21	theme	droplets	928:935	arg1	size					911:914	size	911:914	size	911:914	The results showed that hydroxypropyl-β-cyclodextrin (HP-β-CD), rather than solid carriers commonly used in solidification of traditional Pickering emulsions, was suitable for the solid NSSPE to retain the original appearance and size of emulsion droplets after reconstitution.
33806952	5	21	theme	droplets	928:935	arg1	appearance					896:905	original appearance	887:905	original appearance	887:905	The results showed that hydroxypropyl-β-cyclodextrin (HP-β-CD), rather than solid carriers commonly used in solidification of traditional Pickering emulsions, was suitable for the solid NSSPE to retain the original appearance and size of emulsion droplets after reconstitution.
33806952	8	22	theme	particle	1358:1365	arg1	size					1367:1370	The particle size	1354:1370	The particle size of puerarin nanocrystal sand interfacial adsorption rate	1354:1427	The particle size of puerarin nanocrystal sand interfacial adsorption rate also did not change significantly.
33806952	5	23	theme	Pickering	819:827	arg1	emulsions					829:837	traditional Pickering emulsions	807:837	traditional Pickering emulsions	807:837	The results showed that hydroxypropyl-β-cyclodextrin (HP-β-CD), rather than solid carriers commonly used in solidification of traditional Pickering emulsions, was suitable for the solid NSSPE to retain the original appearance and size of emulsion droplets after reconstitution.
33806952	9	24	theme	liquid	1576:1581	arg1	NSSPE					1583:1587	the liquid NSSPE	1572:1587	the liquid NSSPE	1572:1587	The cumulative amount of released puerarin from the solid NSSPE had no significant difference compared with the liquid NSSPE, which were both significantly higher than that of puerarin crude material.
33806952	9	24	theme	liquid	1576:1581	arg1	higher					1620:1625	higher	1620:1625	higher	1620:1625	The cumulative amount of released puerarin from the solid NSSPE had no significant difference compared with the liquid NSSPE, which were both significantly higher than that of puerarin crude material.
33806952	6	25	theme	feed	1029:1032	arg1	rate					1034:1037	the feed rate	1025:1037	the feed rate	1025:1037	The amount of HP-β-CD had more influences on the solid NSSPE than the feed rate and the inlet air temperature.
33806952	7	26	theme	same	1251:1254	arg1	structure					1267:1275	the same core-shell structure	1247:1275	the same core-shell structure	1247:1275	Fluorescence microscopy, confocal laser scanning microscopy and scanning electron microscopy showed that the reconstituted emulsion of the solid NSSPE prepared with HP-β-CD had the same core-shell structure with a core of oil and a shell of puerarin nanocrystals as the liquid NSSPE.
33806952	8	27	theme	nanocrystal	1384:1394	arg1	rate					1424:1427	puerarin nanocrystal sand interfacial adsorption rate	1375:1427	puerarin nanocrystal sand interfacial adsorption rate	1375:1427	The particle size of puerarin nanocrystal sand interfacial adsorption rate also did not change significantly.
33806952	2	28	theme	NSSPE	429:433	arg1	microstructure					411:424	the original microstructure	398:424	the original microstructure of NSSPE by spray-drying	398:449	This study aimed to develop a new solid NSSPE of puerarin preserving the original microstructure of NSSPE by spray-drying.
33806952	0	29	theme	Pickering	43:51	arg1	Emulsion					53:60	A Novel Solid Nanocrystals Self-Stabilized Pickering Emulsion	0:60	A Novel Solid Nanocrystals Self-Stabilized Pickering Emulsion	0:60	A Novel Solid Nanocrystals Self-Stabilized Pickering Emulsion Prepared by Spray-Drying with Hydroxypropyl-β-cyclodextrin as Carriers.
33806952	8	30	theme	interfacial	1401:1411	arg1	rate					1424:1427	puerarin nanocrystal sand interfacial adsorption rate	1375:1427	puerarin nanocrystal sand interfacial adsorption rate	1375:1427	The particle size of puerarin nanocrystal sand interfacial adsorption rate also did not change significantly.
33806952	1	31	theme	soluble	314:320	arg1	drugs					322:326	poorly soluble drugs	307:326	poorly soluble drugs	307:326	A drug nanocrystals self-stabilized Pickering emulsion (NSSPE) with a unique composition and microstructure has been proven to significantly increase the bioavailability of poorly soluble drugs.
33806952	8	32	theme	rate	1424:1427	arg1	size					1367:1370	The particle size	1354:1370	The particle size of puerarin nanocrystal sand interfacial adsorption rate	1354:1427	The particle size of puerarin nanocrystal sand interfacial adsorption rate also did not change significantly.
33806952	0	33	theme	Solid	8:12	arg1	Emulsion					53:60	A Novel Solid Nanocrystals Self-Stabilized Pickering Emulsion	0:60	A Novel Solid Nanocrystals Self-Stabilized Pickering Emulsion	0:60	A Novel Solid Nanocrystals Self-Stabilized Pickering Emulsion Prepared by Spray-Drying with Hydroxypropyl-β-cyclodextrin as Carriers.
33806952	7	34	theme	liquid	1340:1345	arg1	NSSPE					1347:1351	the liquid NSSPE	1336:1351	the liquid NSSPE	1336:1351	Fluorescence microscopy, confocal laser scanning microscopy and scanning electron microscopy showed that the reconstituted emulsion of the solid NSSPE prepared with HP-β-CD had the same core-shell structure with a core of oil and a shell of puerarin nanocrystals as the liquid NSSPE.
33806952	3	35	theme	carriers	484:491	arg1	series					454:459	A series	452:459	A series of water-soluble solid carriers	452:491	A series of water-soluble solid carriers were compared and then Box-Behnken design was used to optimize the parameters of spray-drying.
33806952	0	36	theme	Self-Stabilized	27:41	arg1	Emulsion					53:60	A Novel Solid Nanocrystals Self-Stabilized Pickering Emulsion	0:60	A Novel Solid Nanocrystals Self-Stabilized Pickering Emulsion	0:60	A Novel Solid Nanocrystals Self-Stabilized Pickering Emulsion Prepared by Spray-Drying with Hydroxypropyl-β-cyclodextrin as Carriers.
33806952	5	37	dep	appearance	896:905	arg1	the					883:885	the	883:885	the	883:885	The results showed that hydroxypropyl-β-cyclodextrin (HP-β-CD), rather than solid carriers commonly used in solidification of traditional Pickering emulsions, was suitable for the solid NSSPE to retain the original appearance and size of emulsion droplets after reconstitution.
33806952	10	38	theme	75	1740:1741	arg1	%					1742:1742	%	1742:1742	%	1742:1742	The solid NSSPE was stable for 3 months under the accelerated condition of 75% relative humidity and 40 °C. Thus, it is possible todevelop the solid NSSPE preserving the unique microstructure and the superior properties in vitro of the liquid NSSPE for poorly soluble drugs.
33806952	9	39	theme	crude	1649:1653	arg1	material					1655:1662	puerarin crude material	1640:1662	puerarin crude material	1640:1662	The cumulative amount of released puerarin from the solid NSSPE had no significant difference compared with the liquid NSSPE, which were both significantly higher than that of puerarin crude material.
33806952	7	40	theme	puerarin	1311:1318	arg1	nanocrystals					1320:1331	puerarin nanocrystals	1311:1331	puerarin nanocrystals as the liquid NSSPE	1311:1351	Fluorescence microscopy, confocal laser scanning microscopy and scanning electron microscopy showed that the reconstituted emulsion of the solid NSSPE prepared with HP-β-CD had the same core-shell structure with a core of oil and a shell of puerarin nanocrystals as the liquid NSSPE.
33806952	2	41	theme	new	359:361	arg1	NSSPE					369:373	a new solid NSSPE	357:373	a new solid NSSPE of puerarin	357:385	This study aimed to develop a new solid NSSPE of puerarin preserving the original microstructure of NSSPE by spray-drying.
33806952	7	42	theme	shell	1302:1306	arg1	core					1284:1287	a core	1282:1287	a core of oil and a shell of puerarin nanocrystals as the liquid NSSPE	1282:1351	Fluorescence microscopy, confocal laser scanning microscopy and scanning electron microscopy showed that the reconstituted emulsion of the solid NSSPE prepared with HP-β-CD had the same core-shell structure with a core of oil and a shell of puerarin nanocrystals as the liquid NSSPE.
33806952	4	43	theme	solid	636:640	arg1	NSSPE					642:646	the optimized solid NSSPE	622:646	the optimized solid NSSPE	622:646	The drug release and stability of the optimized solid NSSPE in vitro were also investigated.
33806952	9	44	from	amount	1479:1484	arg1	NSSPE					1522:1526	the solid NSSPE	1512:1526	the solid NSSPE	1512:1526	The cumulative amount of released puerarin from the solid NSSPE had no significant difference compared with the liquid NSSPE, which were both significantly higher than that of puerarin crude material.
33806952	7	45	theme	core-shell	1256:1265	arg1	structure					1267:1275	the same core-shell structure	1247:1275	the same core-shell structure	1247:1275	Fluorescence microscopy, confocal laser scanning microscopy and scanning electron microscopy showed that the reconstituted emulsion of the solid NSSPE prepared with HP-β-CD had the same core-shell structure with a core of oil and a shell of puerarin nanocrystals as the liquid NSSPE.
33806952	2	46	theme	puerarin	378:385	arg1	NSSPE					369:373	a new solid NSSPE	357:373	a new solid NSSPE of puerarin	357:385	This study aimed to develop a new solid NSSPE of puerarin preserving the original microstructure of NSSPE by spray-drying.
33806952	10	47	theme	NSSPE	1908:1912	arg1	properties					1874:1883	the superior properties	1861:1883	the superior properties in vitro	1861:1892	The solid NSSPE was stable for 3 months under the accelerated condition of 75% relative humidity and 40 °C. Thus, it is possible todevelop the solid NSSPE preserving the unique microstructure and the superior properties in vitro of the liquid NSSPE for poorly soluble drugs.
33806952	10	47	theme	NSSPE	1908:1912	arg1	microstructure					1842:1855	the unique microstructure	1831:1855	the unique microstructure	1831:1855	The solid NSSPE was stable for 3 months under the accelerated condition of 75% relative humidity and 40 °C. Thus, it is possible todevelop the solid NSSPE preserving the unique microstructure and the superior properties in vitro of the liquid NSSPE for poorly soluble drugs.
33806952	3	48	theme	spray-drying	574:585	arg1	parameters					560:569	the parameters	556:569	the parameters of spray-drying	556:585	A series of water-soluble solid carriers were compared and then Box-Behnken design was used to optimize the parameters of spray-drying.
33806952	7	49	theme	scanning	1134:1141	arg1	microscopy					1152:1161	scanning electron microscopy	1134:1161	scanning electron microscopy	1134:1161	Fluorescence microscopy, confocal laser scanning microscopy and scanning electron microscopy showed that the reconstituted emulsion of the solid NSSPE prepared with HP-β-CD had the same core-shell structure with a core of oil and a shell of puerarin nanocrystals as the liquid NSSPE.
33806952	10	50	theme	°C.	1769:1771	arg1	condition					1727:1735	the accelerated condition	1711:1735	the accelerated condition of 75% relative humidity and 40 °C.	1711:1771	The solid NSSPE was stable for 3 months under the accelerated condition of 75% relative humidity and 40 °C. Thus, it is possible todevelop the solid NSSPE preserving the unique microstructure and the superior properties in vitro of the liquid NSSPE for poorly soluble drugs.
33806952	1	51	theme	unique	204:209	arg1	composition					211:221	a unique composition	202:221	a unique composition	202:221	A drug nanocrystals self-stabilized Pickering emulsion (NSSPE) with a unique composition and microstructure has been proven to significantly increase the bioavailability of poorly soluble drugs.
33806952	9	52	theme	released	1489:1496	arg1	puerarin					1498:1505	released puerarin	1489:1505	released puerarin from the solid NSSPE	1489:1526	The cumulative amount of released puerarin from the solid NSSPE had no significant difference compared with the liquid NSSPE, which were both significantly higher than that of puerarin crude material.
33806952	7	53	theme	laser	1104:1108	arg1	microscopy					1119:1128	confocal laser scanning microscopy	1095:1128	confocal laser scanning microscopy	1095:1128	Fluorescence microscopy, confocal laser scanning microscopy and scanning electron microscopy showed that the reconstituted emulsion of the solid NSSPE prepared with HP-β-CD had the same core-shell structure with a core of oil and a shell of puerarin nanocrystals as the liquid NSSPE.
33806952	10	54	theme	solid	1669:1673	arg1	stable					1685:1690	stable	1685:1690	stable	1685:1690	The solid NSSPE was stable for 3 months under the accelerated condition of 75% relative humidity and 40 °C. Thus, it is possible todevelop the solid NSSPE preserving the unique microstructure and the superior properties in vitro of the liquid NSSPE for poorly soluble drugs.
33806952	10	54	theme	solid	1669:1673	arg1	NSSPE					1675:1679	The solid NSSPE	1665:1679	The solid NSSPE	1665:1679	The solid NSSPE was stable for 3 months under the accelerated condition of 75% relative humidity and 40 °C. Thus, it is possible todevelop the solid NSSPE preserving the unique microstructure and the superior properties in vitro of the liquid NSSPE for poorly soluble drugs.
33806952	9	55	theme	solid	1516:1520	arg1	NSSPE					1522:1526	the solid NSSPE	1512:1526	the solid NSSPE	1512:1526	The cumulative amount of released puerarin from the solid NSSPE had no significant difference compared with the liquid NSSPE, which were both significantly higher than that of puerarin crude material.
33806952	7	56	theme	Fluorescence	1070:1081	arg1	microscopy					1083:1092	Fluorescence microscopy	1070:1092	Fluorescence microscopy	1070:1092	Fluorescence microscopy, confocal laser scanning microscopy and scanning electron microscopy showed that the reconstituted emulsion of the solid NSSPE prepared with HP-β-CD had the same core-shell structure with a core of oil and a shell of puerarin nanocrystals as the liquid NSSPE.
33806952	10	57	theme	solid	1808:1812	arg1	NSSPE					1814:1818	the solid NSSPE	1804:1818	the solid NSSPE	1804:1818	The solid NSSPE was stable for 3 months under the accelerated condition of 75% relative humidity and 40 °C. Thus, it is possible todevelop the solid NSSPE preserving the unique microstructure and the superior properties in vitro of the liquid NSSPE for poorly soluble drugs.
33806952	1	58	theme	drug	136:139	arg1	nanocrystals					141:152	drug nanocrystals	136:152	A drug nanocrystals self-stabilized Pickering emulsion (NSSPE) with a unique composition and microstructure	134:240	A drug nanocrystals self-stabilized Pickering emulsion (NSSPE) with a unique composition and microstructure has been proven to significantly increase the bioavailability of poorly soluble drugs.
33806952	9	59	theme	significant	1535:1545	arg1	difference					1547:1556	no significant difference	1532:1556	no significant difference	1532:1556	The cumulative amount of released puerarin from the solid NSSPE had no significant difference compared with the liquid NSSPE, which were both significantly higher than that of puerarin crude material.
33806952	6	60	theme	air	1053:1055	arg1	temperature					1057:1067	the inlet air temperature	1043:1067	the inlet air temperature	1043:1067	The amount of HP-β-CD had more influences on the solid NSSPE than the feed rate and the inlet air temperature.
33806952	10	61	theme	unique	1835:1840	arg1	microstructure					1842:1855	the unique microstructure	1831:1855	the unique microstructure	1831:1855	The solid NSSPE was stable for 3 months under the accelerated condition of 75% relative humidity and 40 °C. Thus, it is possible todevelop the solid NSSPE preserving the unique microstructure and the superior properties in vitro of the liquid NSSPE for poorly soluble drugs.
33806952	5	62	theme	emulsion	919:926	arg1	droplets					928:935	emulsion droplets	919:935	emulsion droplets	919:935	The results showed that hydroxypropyl-β-cyclodextrin (HP-β-CD), rather than solid carriers commonly used in solidification of traditional Pickering emulsions, was suitable for the solid NSSPE to retain the original appearance and size of emulsion droplets after reconstitution.
33806952	5	63	theme	traditional	807:817	arg1	emulsions					829:837	traditional Pickering emulsions	807:837	traditional Pickering emulsions	807:837	The results showed that hydroxypropyl-β-cyclodextrin (HP-β-CD), rather than solid carriers commonly used in solidification of traditional Pickering emulsions, was suitable for the solid NSSPE to retain the original appearance and size of emulsion droplets after reconstitution.
33806952	3	64	used	used	539:542	arg2	design					528:533	then Box-Behnken design	511:533	then Box-Behnken design	511:533	A series of water-soluble solid carriers were compared and then Box-Behnken design was used to optimize the parameters of spray-drying.
33806952	1	65	with	emulsion	180:187	arg1	microstructure					227:240	microstructure	227:240	microstructure	227:240	A drug nanocrystals self-stabilized Pickering emulsion (NSSPE) with a unique composition and microstructure has been proven to significantly increase the bioavailability of poorly soluble drugs.
33806952	1	65	with	emulsion	180:187	arg1	composition					211:221	a unique composition	202:221	a unique composition	202:221	A drug nanocrystals self-stabilized Pickering emulsion (NSSPE) with a unique composition and microstructure has been proven to significantly increase the bioavailability of poorly soluble drugs.
33806952	10	66	theme	accelerated	1715:1725	arg1	condition					1727:1735	the accelerated condition	1711:1735	the accelerated condition of 75% relative humidity and 40 °C.	1711:1771	The solid NSSPE was stable for 3 months under the accelerated condition of 75% relative humidity and 40 °C. Thus, it is possible todevelop the solid NSSPE preserving the unique microstructure and the superior properties in vitro of the liquid NSSPE for poorly soluble drugs.
33806952	4	67	dep	release	597:603	arg1	The					588:590	The	588:590	The	588:590	The drug release and stability of the optimized solid NSSPE in vitro were also investigated.
33806952	8	68	theme	puerarin	1375:1382	arg1	rate					1424:1427	puerarin nanocrystal sand interfacial adsorption rate	1375:1427	puerarin nanocrystal sand interfacial adsorption rate	1375:1427	The particle size of puerarin nanocrystal sand interfacial adsorption rate also did not change significantly.
33806952	3	69	theme	solid	478:482	arg1	carriers					484:491	water-soluble solid carriers	464:491	water-soluble solid carriers	464:491	A series of water-soluble solid carriers were compared and then Box-Behnken design was used to optimize the parameters of spray-drying.
33806952	0	70	theme	Novel	2:6	arg1	Emulsion					53:60	A Novel Solid Nanocrystals Self-Stabilized Pickering Emulsion	0:60	A Novel Solid Nanocrystals Self-Stabilized Pickering Emulsion	0:60	A Novel Solid Nanocrystals Self-Stabilized Pickering Emulsion Prepared by Spray-Drying with Hydroxypropyl-β-cyclodextrin as Carriers.
33806952	4	71	theme	drug	592:595	arg1	release					597:603	drug release	592:603	drug release	592:603	The drug release and stability of the optimized solid NSSPE in vitro were also investigated.
33806952	8	72	theme	sand	1396:1399	arg1	rate					1424:1427	puerarin nanocrystal sand interfacial adsorption rate	1375:1427	puerarin nanocrystal sand interfacial adsorption rate	1375:1427	The particle size of puerarin nanocrystal sand interfacial adsorption rate also did not change significantly.
33806952	2	73	theme	original	402:409	arg1	microstructure					411:424	the original microstructure	398:424	the original microstructure of NSSPE by spray-drying	398:449	This study aimed to develop a new solid NSSPE of puerarin preserving the original microstructure of NSSPE by spray-drying.
33806952	7	74	theme	solid	1209:1213	arg1	NSSPE					1215:1219	the solid NSSPE	1205:1219	the solid NSSPE prepared with HP-β-CD	1205:1241	Fluorescence microscopy, confocal laser scanning microscopy and scanning electron microscopy showed that the reconstituted emulsion of the solid NSSPE prepared with HP-β-CD had the same core-shell structure with a core of oil and a shell of puerarin nanocrystals as the liquid NSSPE.
33806952	1	75	theme	drugs	322:326	arg1	bioavailability					288:302	the bioavailability	284:302	the bioavailability of poorly soluble drugs	284:326	A drug nanocrystals self-stabilized Pickering emulsion (NSSPE) with a unique composition and microstructure has been proven to significantly increase the bioavailability of poorly soluble drugs.
33806952	8	76	theme	adsorption	1413:1422	arg1	rate					1424:1427	puerarin nanocrystal sand interfacial adsorption rate	1375:1427	puerarin nanocrystal sand interfacial adsorption rate	1375:1427	The particle size of puerarin nanocrystal sand interfacial adsorption rate also did not change significantly.
33806952	5	77	theme	emulsions	829:837	arg1	solidification					789:802	solidification	789:802	solidification of traditional Pickering emulsions	789:837	The results showed that hydroxypropyl-β-cyclodextrin (HP-β-CD), rather than solid carriers commonly used in solidification of traditional Pickering emulsions, was suitable for the solid NSSPE to retain the original appearance and size of emulsion droplets after reconstitution.
33806952	7	78	theme	nanocrystals	1320:1331	arg1	oil					1292:1294	oil	1292:1294	oil	1292:1294	Fluorescence microscopy, confocal laser scanning microscopy and scanning electron microscopy showed that the reconstituted emulsion of the solid NSSPE prepared with HP-β-CD had the same core-shell structure with a core of oil and a shell of puerarin nanocrystals as the liquid NSSPE.
33806952	7	78	theme	nanocrystals	1320:1331	arg1	shell					1302:1306	a shell	1300:1306	a shell of puerarin nanocrystals as the liquid NSSPE	1300:1351	Fluorescence microscopy, confocal laser scanning microscopy and scanning electron microscopy showed that the reconstituted emulsion of the solid NSSPE prepared with HP-β-CD had the same core-shell structure with a core of oil and a shell of puerarin nanocrystals as the liquid NSSPE.
33806952	3	79	theme	water-soluble	464:476	arg1	carriers					484:491	water-soluble solid carriers	464:491	water-soluble solid carriers	464:491	A series of water-soluble solid carriers were compared and then Box-Behnken design was used to optimize the parameters of spray-drying.
33806952	9	80	contain	had	1528:1530	arg1	amount					1479:1484	The cumulative amount	1464:1484	The cumulative amount of released puerarin from the solid NSSPE	1464:1526	The cumulative amount of released puerarin from the solid NSSPE had no significant difference compared with the liquid NSSPE, which were both significantly higher than that of puerarin crude material.
33806952	9	80	contain	had	1528:1530	arg1	puerarin					1498:1505	released puerarin	1489:1505	released puerarin from the solid NSSPE	1489:1526	The cumulative amount of released puerarin from the solid NSSPE had no significant difference compared with the liquid NSSPE, which were both significantly higher than that of puerarin crude material.
33806952	9	80	contain	had	1528:1530	arg2	difference					1547:1556	no significant difference	1532:1556	no significant difference	1532:1556	The cumulative amount of released puerarin from the solid NSSPE had no significant difference compared with the liquid NSSPE, which were both significantly higher than that of puerarin crude material.
33806952	10	81	theme	superior	1865:1872	arg1	properties					1874:1883	the superior properties	1861:1883	the superior properties in vitro	1861:1892	The solid NSSPE was stable for 3 months under the accelerated condition of 75% relative humidity and 40 °C. Thus, it is possible todevelop the solid NSSPE preserving the unique microstructure and the superior properties in vitro of the liquid NSSPE for poorly soluble drugs.
33806952	7	82	contain	had	1243:1245	arg2	structure					1267:1275	the same core-shell structure	1247:1275	the same core-shell structure	1247:1275	Fluorescence microscopy, confocal laser scanning microscopy and scanning electron microscopy showed that the reconstituted emulsion of the solid NSSPE prepared with HP-β-CD had the same core-shell structure with a core of oil and a shell of puerarin nanocrystals as the liquid NSSPE.
33806952	7	82	contain	had	1243:1245	arg1	emulsion					1193:1200	the reconstituted emulsion	1175:1200	the reconstituted emulsion of the solid NSSPE prepared with HP-β-CD	1175:1241	Fluorescence microscopy, confocal laser scanning microscopy and scanning electron microscopy showed that the reconstituted emulsion of the solid NSSPE prepared with HP-β-CD had the same core-shell structure with a core of oil and a shell of puerarin nanocrystals as the liquid NSSPE.
33806952	7	83	theme	reconstituted	1179:1191	arg1	emulsion					1193:1200	the reconstituted emulsion	1175:1200	the reconstituted emulsion of the solid NSSPE prepared with HP-β-CD	1175:1241	Fluorescence microscopy, confocal laser scanning microscopy and scanning electron microscopy showed that the reconstituted emulsion of the solid NSSPE prepared with HP-β-CD had the same core-shell structure with a core of oil and a shell of puerarin nanocrystals as the liquid NSSPE.
33806952	9	84	theme	puerarin	1640:1647	arg1	material					1655:1662	puerarin crude material	1640:1662	puerarin crude material	1640:1662	The cumulative amount of released puerarin from the solid NSSPE had no significant difference compared with the liquid NSSPE, which were both significantly higher than that of puerarin crude material.
33806952	9	85	from	NSSPE	1522:1526	arg1	amount					1479:1484	The cumulative amount	1464:1484	The cumulative amount of released puerarin from the solid NSSPE	1464:1526	The cumulative amount of released puerarin from the solid NSSPE had no significant difference compared with the liquid NSSPE, which were both significantly higher than that of puerarin crude material.
33806952	9	85	from	NSSPE	1522:1526	arg1	puerarin					1498:1505	released puerarin	1489:1505	released puerarin from the solid NSSPE	1489:1526	The cumulative amount of released puerarin from the solid NSSPE had no significant difference compared with the liquid NSSPE, which were both significantly higher than that of puerarin crude material.
33806952	7	86	theme	confocal	1095:1102	arg1	microscopy					1119:1128	confocal laser scanning microscopy	1095:1128	confocal laser scanning microscopy	1095:1128	Fluorescence microscopy, confocal laser scanning microscopy and scanning electron microscopy showed that the reconstituted emulsion of the solid NSSPE prepared with HP-β-CD had the same core-shell structure with a core of oil and a shell of puerarin nanocrystals as the liquid NSSPE.
33806952	10	87	theme	liquid	1901:1906	arg1	NSSPE					1908:1912	the liquid NSSPE	1897:1912	the liquid NSSPE for poorly soluble drugs	1897:1937	The solid NSSPE was stable for 3 months under the accelerated condition of 75% relative humidity and 40 °C. Thus, it is possible todevelop the solid NSSPE preserving the unique microstructure and the superior properties in vitro of the liquid NSSPE for poorly soluble drugs.
33806952	2	88	theme	solid	363:367	arg1	NSSPE					369:373	a new solid NSSPE	357:373	a new solid NSSPE of puerarin	357:385	This study aimed to develop a new solid NSSPE of puerarin preserving the original microstructure of NSSPE by spray-drying.
33806952	4	89	theme	NSSPE	642:646	arg1	stability					609:617	stability	609:617	stability	609:617	The drug release and stability of the optimized solid NSSPE in vitro were also investigated.
33806952	4	89	theme	NSSPE	642:646	arg1	release					597:603	drug release	592:603	drug release	592:603	The drug release and stability of the optimized solid NSSPE in vitro were also investigated.
33806952	6	90	contain	had	981:983	arg2	influences					990:999	more influences	985:999	more influences	985:999	The amount of HP-β-CD had more influences on the solid NSSPE than the feed rate and the inlet air temperature.
33806952	6	90	contain	had	981:983	arg1	HP-β-CD					973:979	HP-β-CD	973:979	HP-β-CD	973:979	The amount of HP-β-CD had more influences on the solid NSSPE than the feed rate and the inlet air temperature.
33806952	6	90	contain	had	981:983	arg1	amount					963:968	The amount	959:968	The amount of HP-β-CD	959:979	The amount of HP-β-CD had more influences on the solid NSSPE than the feed rate and the inlet air temperature.
33806952	3	91	theme	Box-Behnken	516:526	arg1	design					528:533	then Box-Behnken design	511:533	then Box-Behnken design	511:533	A series of water-soluble solid carriers were compared and then Box-Behnken design was used to optimize the parameters of spray-drying.
33806952	5	92	theme	original	887:894	arg1	appearance					896:905	original appearance	887:905	original appearance	887:905	The results showed that hydroxypropyl-β-cyclodextrin (HP-β-CD), rather than solid carriers commonly used in solidification of traditional Pickering emulsions, was suitable for the solid NSSPE to retain the original appearance and size of emulsion droplets after reconstitution.
33806952	7	93	theme	oil	1292:1294	arg1	core					1284:1287	a core	1282:1287	a core of oil and a shell of puerarin nanocrystals as the liquid NSSPE	1282:1351	Fluorescence microscopy, confocal laser scanning microscopy and scanning electron microscopy showed that the reconstituted emulsion of the solid NSSPE prepared with HP-β-CD had the same core-shell structure with a core of oil and a shell of puerarin nanocrystals as the liquid NSSPE.
33806952	10	94	theme	soluble	1925:1931	arg1	drugs					1933:1937	poorly soluble drugs	1918:1937	poorly soluble drugs	1918:1937	The solid NSSPE was stable for 3 months under the accelerated condition of 75% relative humidity and 40 °C. Thus, it is possible todevelop the solid NSSPE preserving the unique microstructure and the superior properties in vitro of the liquid NSSPE for poorly soluble drugs.
33806952	10	95	theme	humidity	1753:1760	arg1	condition					1727:1735	the accelerated condition	1711:1735	the accelerated condition of 75% relative humidity and 40 °C.	1711:1771	The solid NSSPE was stable for 3 months under the accelerated condition of 75% relative humidity and 40 °C. Thus, it is possible todevelop the solid NSSPE preserving the unique microstructure and the superior properties in vitro of the liquid NSSPE for poorly soluble drugs.
33572104	8	0	theme	catalytic	1698:1706	arg1	applications					1708:1719	catalytic applications	1698:1719	catalytic applications in hydrogenation reactions	1698:1746	This work offers an effective approach to the utilization of cyclodextrins for engineering the surface morphology of Ru nanocrystals and pore characteristics of TiO2-based materials for catalytic applications in hydrogenation reactions.
33572104	6	1	theme	LA	1371:1372	arg1	conversion					1374:1383	almost quantitative LA conversion	1351:1383	almost quantitative LA conversion	1351:1383	Results showed that the cyclodextrin-derived catalyst displayed almost quantitative LA conversion and 99% GVL yield in less than one hour.
33572104	5	2	theme	bar	1278:1280	arg1	H2					1282:1283	50 bar H2	1275:1283	50 bar H2	1275:1283	Their catalytic performance was then evaluated in the aqueous phase hydrogenation of levulinic acid (LA) to γ-valerolactone (GVL) under mild conditions (50 °C, 50 bar H2).
33572104	5	2	theme	bar	1278:1280	arg1	°C					1271:1272	50 °C	1268:1272	50 °C	1268:1272	Their catalytic performance was then evaluated in the aqueous phase hydrogenation of levulinic acid (LA) to γ-valerolactone (GVL) under mild conditions (50 °C, 50 bar H2).
33572104	1	3	theme	mesoporous	302:311	arg1	catalysts					328:336	composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts	284:336	composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts	284:336	In this paper, we present a versatile template-directed colloidal self-assembly method for the fabrication in aqueous phase of composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts.
33572104	3	4	theme	electron	829:836	arg1	microscopy					838:847	dark-field scanning transmission electron microscopy	796:847	dark-field scanning transmission electron microscopy	796:847	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	3	5	theme	dynamic	600:606	arg1	diffraction					663:673	powder X-ray diffraction	650:673	powder X-ray diffraction	650:673	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	3	5	theme	dynamic	600:606	arg1	mapping					878:884	EDS elemental mapping	864:884	EDS elemental mapping	864:884	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	3	5	theme	dynamic	600:606	arg1	microscopy					748:757	high-resolution transmission electron microscopy	710:757	high-resolution transmission electron microscopy	710:757	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	3	5	theme	dynamic	600:606	arg1	analysis					640:647	N2-adsorption analysis	626:647	N2-adsorption analysis	626:647	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	3	5	theme	dynamic	600:606	arg1	reduction					699:707	temperature programmed reduction	676:707	temperature programmed reduction	676:707	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	3	5	theme	dynamic	600:606	arg1	scattering					614:623	dynamic light scattering	600:623	dynamic light scattering	600:623	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	3	5	theme	dynamic	600:606	arg1	bright-field					779:790	high-angle annular bright-field	760:790	high-angle annular bright-field	760:790	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	7	6	theme	activity	1502:1509	arg1	loss					1494:1497	loss	1494:1497	loss of activity	1494:1509	Moreover, this catalyst could be reused at least five times without loss of activity.
33572104	8	7	from	applications	1708:1719	arg1	reactions					1738:1746	hydrogenation reactions	1724:1746	hydrogenation reactions	1724:1746	This work offers an effective approach to the utilization of cyclodextrins for engineering the surface morphology of Ru nanocrystals and pore characteristics of TiO2-based materials for catalytic applications in hydrogenation reactions.
33572104	3	8	theme	elemental	868:876	arg1	scattering					614:623	dynamic light scattering	600:623	dynamic light scattering	600:623	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	3	8	theme	elemental	868:876	arg1	mapping					878:884	EDS elemental mapping	864:884	EDS elemental mapping	864:884	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	2	9	theme	methylated	348:357	arg1	assemblies					403:412	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies	339:412	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies	339:412	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	2	9	theme	methylated	348:357	arg1	source					518:523	a silica source	509:523	a silica source	509:523	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	2	9	theme	methylated	348:357	arg1	colloids					448:455	TiO2 colloids	443:455	TiO2 colloids as building blocks	443:474	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	2	9	theme	methylated	348:357	arg1	orthosilicate					492:504	tetraethyl orthosilicate	481:504	tetraethyl orthosilicate	481:504	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	2	9	theme	methylated	348:357	arg1	templates					432:440	soft templates	427:440	soft templates	427:440	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	1	10	theme	template-directed	195:211	arg1	method					237:242	a versatile template-directed colloidal self-assembly method	183:242	a versatile template-directed colloidal self-assembly method for the fabrication in aqueous phase of composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts	183:336	In this paper, we present a versatile template-directed colloidal self-assembly method for the fabrication in aqueous phase of composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts.
33572104	4	11	theme	mixed	1046:1050	arg1	oxides					1052:1057	the mixed oxides	1042:1057	the mixed oxides	1042:1057	Results revealed that both the supramolecular template and the silica loading had a strong impact on the pore characteristics and crystalline structure of the mixed oxides, as well as on the morphology of the RuO2 nanocrystals.
33572104	5	12	theme	phase	1177:1181	arg1	hydrogenation					1183:1195	the aqueous phase hydrogenation	1165:1195	the aqueous phase hydrogenation of levulinic acid (LA) to γ-valerolactone (GVL) under mild conditions (50 °C, 50 bar H2)	1165:1284	Their catalytic performance was then evaluated in the aqueous phase hydrogenation of levulinic acid (LA) to γ-valerolactone (GVL) under mild conditions (50 °C, 50 bar H2).
33572104	3	13	theme	N2-adsorption	626:638	arg1	scattering					614:623	dynamic light scattering	600:623	dynamic light scattering	600:623	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	3	13	theme	N2-adsorption	626:638	arg1	analysis					640:647	N2-adsorption analysis	626:647	N2-adsorption analysis	626:647	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	1	14	theme	self-assembly	223:235	arg1	method					237:242	a versatile template-directed colloidal self-assembly method	183:242	a versatile template-directed colloidal self-assembly method for the fabrication in aqueous phase of composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts	183:336	In this paper, we present a versatile template-directed colloidal self-assembly method for the fabrication in aqueous phase of composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts.
33572104	4	15	theme	crystalline	1017:1027	arg1	structure					1029:1037	crystalline structure	1017:1037	crystalline structure	1017:1037	Results revealed that both the supramolecular template and the silica loading had a strong impact on the pore characteristics and crystalline structure of the mixed oxides, as well as on the morphology of the RuO2 nanocrystals.
33572104	6	16	theme	%	1391:1391	arg1	yield					1397:1401	99% GVL yield	1389:1401	99% GVL yield	1389:1401	Results showed that the cyclodextrin-derived catalyst displayed almost quantitative LA conversion and 99% GVL yield in less than one hour.
33572104	3	17	theme	X-ray	657:661	arg1	diffraction					663:673	powder X-ray diffraction	650:673	powder X-ray diffraction	650:673	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	3	17	theme	X-ray	657:661	arg1	scattering					614:623	dynamic light scattering	600:623	dynamic light scattering	600:623	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	1	18	from	fabrication	252:262	arg1	phase					275:279	aqueous phase	267:279	aqueous phase of composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts	267:336	In this paper, we present a versatile template-directed colloidal self-assembly method for the fabrication in aqueous phase of composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts.
33572104	4	19	contain	had	965:967	arg1	template					933:940	the supramolecular template	914:940	the supramolecular template	914:940	Results revealed that both the supramolecular template and the silica loading had a strong impact on the pore characteristics and crystalline structure of the mixed oxides, as well as on the morphology of the RuO2 nanocrystals.
33572104	4	19	contain	had	965:967	arg2	impact					978:983	a strong impact	969:983	a strong impact	969:983	Results revealed that both the supramolecular template and the silica loading had a strong impact on the pore characteristics and crystalline structure of the mixed oxides, as well as on the morphology of the RuO2 nanocrystals.
33572104	4	19	contain	had	965:967	arg1	loading					957:963	the silica loading	946:963	the silica loading	946:963	Results revealed that both the supramolecular template and the silica loading had a strong impact on the pore characteristics and crystalline structure of the mixed oxides, as well as on the morphology of the RuO2 nanocrystals.
33572104	0	20	theme	Mixed	84:88	arg1	Oxides					90:95	Mesoporous Cyclodextrin-Templated TiO2-SiO2 Mixed Oxides	40:95	Mesoporous Cyclodextrin-Templated TiO2-SiO2 Mixed Oxides for the Hydrogenation of Levulinic Acid to γ-Valerolactone	40:154	Robust Ruthenium Catalysts Supported on Mesoporous Cyclodextrin-Templated TiO2-SiO2 Mixed Oxides for the Hydrogenation of Levulinic Acid to γ-Valerolactone.
33572104	4	21	from	characteristics	997:1011	arg1	morphology					1078:1087	the morphology	1074:1087	the morphology of the RuO2 nanocrystals	1074:1112	Results revealed that both the supramolecular template and the silica loading had a strong impact on the pore characteristics and crystalline structure of the mixed oxides, as well as on the morphology of the RuO2 nanocrystals.
33572104	3	22	theme	programmed	688:697	arg1	scattering					614:623	dynamic light scattering	600:623	dynamic light scattering	600:623	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	3	22	theme	programmed	688:697	arg1	reduction					699:707	temperature programmed reduction	676:707	temperature programmed reduction	676:707	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	5	23	theme	acid	1210:1213	arg1	hydrogenation					1183:1195	the aqueous phase hydrogenation	1165:1195	the aqueous phase hydrogenation of levulinic acid (LA) to γ-valerolactone (GVL) under mild conditions (50 °C, 50 bar H2)	1165:1284	Their catalytic performance was then evaluated in the aqueous phase hydrogenation of levulinic acid (LA) to γ-valerolactone (GVL) under mild conditions (50 °C, 50 bar H2).
33572104	5	24	dep	conditions	1256:1265	arg1	H2					1282:1283	50 bar H2	1275:1283	50 bar H2	1275:1283	Their catalytic performance was then evaluated in the aqueous phase hydrogenation of levulinic acid (LA) to γ-valerolactone (GVL) under mild conditions (50 °C, 50 bar H2).
33572104	5	24	dep	conditions	1256:1265	arg1	°C					1271:1272	50 °C	1268:1272	50 °C	1268:1272	Their catalytic performance was then evaluated in the aqueous phase hydrogenation of levulinic acid (LA) to γ-valerolactone (GVL) under mild conditions (50 °C, 50 bar H2).
33572104	0	25	theme	Levulinic	122:130	arg1	Acid					132:135	Levulinic Acid	122:135	Levulinic Acid	122:135	Robust Ruthenium Catalysts Supported on Mesoporous Cyclodextrin-Templated TiO2-SiO2 Mixed Oxides for the Hydrogenation of Levulinic Acid to γ-Valerolactone.
33572104	4	26	dep	characteristics	997:1011	arg1	the					988:990	the	988:990	the	988:990	Results revealed that both the supramolecular template and the silica loading had a strong impact on the pore characteristics and crystalline structure of the mixed oxides, as well as on the morphology of the RuO2 nanocrystals.
33572104	2	27	theme	TiO2	443:446	arg1	colloids					448:455	TiO2 colloids	443:455	TiO2 colloids as building blocks	443:474	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	2	27	theme	TiO2	443:446	arg1	assemblies					403:412	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies	339:412	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies	339:412	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	0	28	theme	Robust	0:5	arg1	Catalysts					17:25	Robust Ruthenium Catalysts	0:25	Robust Ruthenium Catalysts	0:25	Robust Ruthenium Catalysts Supported on Mesoporous Cyclodextrin-Templated TiO2-SiO2 Mixed Oxides for the Hydrogenation of Levulinic Acid to γ-Valerolactone.
33572104	1	29	theme	RuO2	313:316	arg1	catalysts					328:336	composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts	284:336	composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts	284:336	In this paper, we present a versatile template-directed colloidal self-assembly method for the fabrication in aqueous phase of composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts.
33572104	2	30	theme	soft	427:430	arg1	assemblies					403:412	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies	339:412	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies	339:412	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	2	30	theme	soft	427:430	arg1	templates					432:440	soft templates	427:440	soft templates	427:440	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	1	31	theme	TiO2-SiO2	318:326	arg1	catalysts					328:336	composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts	284:336	composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts	284:336	In this paper, we present a versatile template-directed colloidal self-assembly method for the fabrication in aqueous phase of composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts.
33572104	4	32	theme	nanocrystals	1101:1112	arg1	morphology					1078:1087	the morphology	1074:1087	the morphology of the RuO2 nanocrystals	1074:1112	Results revealed that both the supramolecular template and the silica loading had a strong impact on the pore characteristics and crystalline structure of the mixed oxides, as well as on the morphology of the RuO2 nanocrystals.
33572104	1	33	attach	present	175:181	arg2	we					172:173	we	172:173	we	172:173	In this paper, we present a versatile template-directed colloidal self-assembly method for the fabrication in aqueous phase of composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts.
33572104	1	33	attach	present	175:181	arg1	paper					165:169	this paper	160:169	this paper	160:169	In this paper, we present a versatile template-directed colloidal self-assembly method for the fabrication in aqueous phase of composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts.
33572104	8	34	theme	Ru	1629:1630	arg1	nanocrystals					1632:1643	Ru nanocrystals	1629:1643	Ru nanocrystals	1629:1643	This work offers an effective approach to the utilization of cyclodextrins for engineering the surface morphology of Ru nanocrystals and pore characteristics of TiO2-based materials for catalytic applications in hydrogenation reactions.
33572104	3	35	theme	transmission	726:737	arg1	scattering					614:623	dynamic light scattering	600:623	dynamic light scattering	600:623	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	3	35	theme	transmission	726:737	arg1	microscopy					748:757	high-resolution transmission electron microscopy	710:757	high-resolution transmission electron microscopy	710:757	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	0	36	theme	Cyclodextrin-Templated	51:72	arg1	Oxides					90:95	Mesoporous Cyclodextrin-Templated TiO2-SiO2 Mixed Oxides	40:95	Mesoporous Cyclodextrin-Templated TiO2-SiO2 Mixed Oxides for the Hydrogenation of Levulinic Acid to γ-Valerolactone	40:154	Robust Ruthenium Catalysts Supported on Mesoporous Cyclodextrin-Templated TiO2-SiO2 Mixed Oxides for the Hydrogenation of Levulinic Acid to γ-Valerolactone.
33572104	5	37	theme	mild	1251:1254	arg1	conditions					1256:1265	mild conditions	1251:1265	mild conditions (50 °C, 50 bar H2)	1251:1284	Their catalytic performance was then evaluated in the aqueous phase hydrogenation of levulinic acid (LA) to γ-valerolactone (GVL) under mild conditions (50 °C, 50 bar H2).
33572104	2	38	theme	supramolecular	388:401	arg1	assemblies					403:412	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies	339:412	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies	339:412	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	2	38	theme	supramolecular	388:401	arg1	source					518:523	a silica source	509:523	a silica source	509:523	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	2	38	theme	supramolecular	388:401	arg1	colloids					448:455	TiO2 colloids	443:455	TiO2 colloids as building blocks	443:474	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	2	38	theme	supramolecular	388:401	arg1	orthosilicate					492:504	tetraethyl orthosilicate	481:504	tetraethyl orthosilicate	481:504	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	2	38	theme	supramolecular	388:401	arg1	templates					432:440	soft templates	427:440	soft templates	427:440	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	8	39	theme	hydrogenation	1724:1736	arg1	reactions					1738:1746	hydrogenation reactions	1724:1746	hydrogenation reactions	1724:1746	This work offers an effective approach to the utilization of cyclodextrins for engineering the surface morphology of Ru nanocrystals and pore characteristics of TiO2-based materials for catalytic applications in hydrogenation reactions.
33572104	8	40	theme	characteristics	1654:1668	arg1	morphology					1615:1624	the surface morphology	1603:1624	the surface morphology of Ru nanocrystals and pore characteristics of TiO2-based materials for catalytic applications in hydrogenation reactions	1603:1746	This work offers an effective approach to the utilization of cyclodextrins for engineering the surface morphology of Ru nanocrystals and pore characteristics of TiO2-based materials for catalytic applications in hydrogenation reactions.
33572104	3	41	theme	high-angle	760:769	arg1	scattering					614:623	dynamic light scattering	600:623	dynamic light scattering	600:623	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	3	41	theme	high-angle	760:769	arg1	bright-field					779:790	high-angle annular bright-field	760:790	high-angle annular bright-field	760:790	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	8	42	theme	TiO2-based	1673:1682	arg1	materials					1684:1692	TiO2-based materials	1673:1692	TiO2-based materials for catalytic applications in hydrogenation reactions	1673:1746	This work offers an effective approach to the utilization of cyclodextrins for engineering the surface morphology of Ru nanocrystals and pore characteristics of TiO2-based materials for catalytic applications in hydrogenation reactions.
33572104	3	43	theme	transmission	816:827	arg1	microscopy					838:847	dark-field scanning transmission electron microscopy	796:847	dark-field scanning transmission electron microscopy	796:847	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	5	44	theme	50	1275:1276	arg1	bar					1278:1280	bar	1278:1280	bar	1278:1280	Their catalytic performance was then evaluated in the aqueous phase hydrogenation of levulinic acid (LA) to γ-valerolactone (GVL) under mild conditions (50 °C, 50 bar H2).
33572104	3	45	theme	dark-field	796:805	arg1	microscopy					838:847	dark-field scanning transmission electron microscopy	796:847	dark-field scanning transmission electron microscopy	796:847	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	6	46	theme	quantitative	1358:1369	arg1	conversion					1374:1383	almost quantitative LA conversion	1351:1383	almost quantitative LA conversion	1351:1383	Results showed that the cyclodextrin-derived catalyst displayed almost quantitative LA conversion and 99% GVL yield in less than one hour.
33572104	3	47	theme	light	608:612	arg1	diffraction					663:673	powder X-ray diffraction	650:673	powder X-ray diffraction	650:673	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	3	47	theme	light	608:612	arg1	mapping					878:884	EDS elemental mapping	864:884	EDS elemental mapping	864:884	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	3	47	theme	light	608:612	arg1	microscopy					748:757	high-resolution transmission electron microscopy	710:757	high-resolution transmission electron microscopy	710:757	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	3	47	theme	light	608:612	arg1	analysis					640:647	N2-adsorption analysis	626:647	N2-adsorption analysis	626:647	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	3	47	theme	light	608:612	arg1	reduction					699:707	temperature programmed reduction	676:707	temperature programmed reduction	676:707	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	3	47	theme	light	608:612	arg1	scattering					614:623	dynamic light scattering	600:623	dynamic light scattering	600:623	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	3	47	theme	light	608:612	arg1	bright-field					779:790	high-angle annular bright-field	760:790	high-angle annular bright-field	760:790	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	1	48	theme	composition-tuned	284:300	arg1	catalysts					328:336	composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts	284:336	composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts	284:336	In this paper, we present a versatile template-directed colloidal self-assembly method for the fabrication in aqueous phase of composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts.
33572104	4	49	theme	pore	992:995	arg1	characteristics					997:1011	pore characteristics	992:1011	pore characteristics	992:1011	Results revealed that both the supramolecular template and the silica loading had a strong impact on the pore characteristics and crystalline structure of the mixed oxides, as well as on the morphology of the RuO2 nanocrystals.
33572104	6	50	theme	cyclodextrin-derived	1311:1330	arg1	catalyst					1332:1339	the cyclodextrin-derived catalyst	1307:1339	the cyclodextrin-derived catalyst	1307:1339	Results showed that the cyclodextrin-derived catalyst displayed almost quantitative LA conversion and 99% GVL yield in less than one hour.
33572104	2	51	theme	β-cyclodextrin/Pluronic	359:381	arg1	assemblies					403:412	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies	339:412	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies	339:412	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	2	51	theme	β-cyclodextrin/Pluronic	359:381	arg1	source					518:523	a silica source	509:523	a silica source	509:523	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	2	51	theme	β-cyclodextrin/Pluronic	359:381	arg1	colloids					448:455	TiO2 colloids	443:455	TiO2 colloids as building blocks	443:474	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	2	51	theme	β-cyclodextrin/Pluronic	359:381	arg1	orthosilicate					492:504	tetraethyl orthosilicate	481:504	tetraethyl orthosilicate	481:504	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	2	51	theme	β-cyclodextrin/Pluronic	359:381	arg1	templates					432:440	soft templates	427:440	soft templates	427:440	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	1	52	theme	versatile	185:193	arg1	method					237:242	a versatile template-directed colloidal self-assembly method	183:242	a versatile template-directed colloidal self-assembly method for the fabrication in aqueous phase of composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts	183:336	In this paper, we present a versatile template-directed colloidal self-assembly method for the fabrication in aqueous phase of composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts.
33572104	4	53	theme	strong	971:976	arg1	impact					978:983	a strong impact	969:983	a strong impact	969:983	Results revealed that both the supramolecular template and the silica loading had a strong impact on the pore characteristics and crystalline structure of the mixed oxides, as well as on the morphology of the RuO2 nanocrystals.
33572104	6	54	link	cyclodextrin-derived	1311:1330	arg1	catalyst					1332:1339	the cyclodextrin-derived catalyst	1307:1339	the cyclodextrin-derived catalyst	1307:1339	Results showed that the cyclodextrin-derived catalyst displayed almost quantitative LA conversion and 99% GVL yield in less than one hour.
33572104	1	55	theme	colloidal	213:221	arg1	method					237:242	a versatile template-directed colloidal self-assembly method	183:242	a versatile template-directed colloidal self-assembly method for the fabrication in aqueous phase of composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts	183:336	In this paper, we present a versatile template-directed colloidal self-assembly method for the fabrication in aqueous phase of composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts.
33572104	3	56	theme	EDS	864:866	arg1	scattering					614:623	dynamic light scattering	600:623	dynamic light scattering	600:623	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	3	56	theme	EDS	864:866	arg1	mapping					878:884	EDS elemental mapping	864:884	EDS elemental mapping	864:884	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	4	57	theme	oxides	1052:1057	arg1	structure					1029:1037	crystalline structure	1017:1037	crystalline structure	1017:1037	Results revealed that both the supramolecular template and the silica loading had a strong impact on the pore characteristics and crystalline structure of the mixed oxides, as well as on the morphology of the RuO2 nanocrystals.
33572104	4	57	theme	oxides	1052:1057	arg1	characteristics					997:1011	pore characteristics	992:1011	pore characteristics	992:1011	Results revealed that both the supramolecular template and the silica loading had a strong impact on the pore characteristics and crystalline structure of the mixed oxides, as well as on the morphology of the RuO2 nanocrystals.
33572104	6	58	theme	GVL	1393:1395	arg1	yield					1397:1401	99% GVL yield	1389:1401	99% GVL yield	1389:1401	Results showed that the cyclodextrin-derived catalyst displayed almost quantitative LA conversion and 99% GVL yield in less than one hour.
33572104	5	59	theme	levulinic	1200:1208	arg1	LA					1216:1217	LA	1216:1217	LA	1216:1217	Their catalytic performance was then evaluated in the aqueous phase hydrogenation of levulinic acid (LA) to γ-valerolactone (GVL) under mild conditions (50 °C, 50 bar H2).
33572104	5	59	theme	levulinic	1200:1208	arg1	acid					1210:1213	levulinic acid	1200:1213	levulinic acid (LA)	1200:1218	Their catalytic performance was then evaluated in the aqueous phase hydrogenation of levulinic acid (LA) to γ-valerolactone (GVL) under mild conditions (50 °C, 50 bar H2).
33572104	3	60	theme	powder	650:655	arg1	diffraction					663:673	powder X-ray diffraction	650:673	powder X-ray diffraction	650:673	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	3	60	theme	powder	650:655	arg1	scattering					614:623	dynamic light scattering	600:623	dynamic light scattering	600:623	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	6	61	theme	99	1389:1390	arg1	%					1391:1391	%	1391:1391	%	1391:1391	Results showed that the cyclodextrin-derived catalyst displayed almost quantitative LA conversion and 99% GVL yield in less than one hour.
33572104	4	62	from	structure	1029:1037	arg1	morphology					1078:1087	the morphology	1074:1087	the morphology of the RuO2 nanocrystals	1074:1112	Results revealed that both the supramolecular template and the silica loading had a strong impact on the pore characteristics and crystalline structure of the mixed oxides, as well as on the morphology of the RuO2 nanocrystals.
33572104	8	63	theme	effective	1532:1540	arg1	approach					1542:1549	an effective approach	1529:1549	an effective approach to the utilization of cyclodextrins for engineering the surface morphology of Ru nanocrystals and pore characteristics of TiO2-based materials for catalytic applications in hydrogenation reactions	1529:1746	This work offers an effective approach to the utilization of cyclodextrins for engineering the surface morphology of Ru nanocrystals and pore characteristics of TiO2-based materials for catalytic applications in hydrogenation reactions.
33572104	5	64	theme	aqueous	1169:1175	arg1	hydrogenation					1183:1195	the aqueous phase hydrogenation	1165:1195	the aqueous phase hydrogenation of levulinic acid (LA) to γ-valerolactone (GVL) under mild conditions (50 °C, 50 bar H2)	1165:1284	Their catalytic performance was then evaluated in the aqueous phase hydrogenation of levulinic acid (LA) to γ-valerolactone (GVL) under mild conditions (50 °C, 50 bar H2).
33572104	3	65	theme	temperature	676:686	arg1	scattering					614:623	dynamic light scattering	600:623	dynamic light scattering	600:623	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	3	65	theme	temperature	676:686	arg1	reduction					699:707	temperature programmed reduction	676:707	temperature programmed reduction	676:707	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	2	66	used	used	419:422	arg2	templates					432:440	soft templates	427:440	soft templates	427:440	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	2	66	used	used	419:422	arg2	source					518:523	a silica source	509:523	a silica source	509:523	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	2	66	used	used	419:422	arg2	colloids					448:455	TiO2 colloids	443:455	TiO2 colloids as building blocks	443:474	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	2	66	used	used	419:422	arg2	orthosilicate					492:504	tetraethyl orthosilicate	481:504	tetraethyl orthosilicate	481:504	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	2	66	used	used	419:422	arg2	assemblies					403:412	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies	339:412	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies	339:412	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	8	67	theme	cyclodextrins	1573:1585	arg1	utilization					1558:1568	the utilization	1554:1568	the utilization of cyclodextrins for engineering the surface morphology of Ru nanocrystals and pore characteristics of TiO2-based materials for catalytic applications in hydrogenation reactions	1554:1746	This work offers an effective approach to the utilization of cyclodextrins for engineering the surface morphology of Ru nanocrystals and pore characteristics of TiO2-based materials for catalytic applications in hydrogenation reactions.
33572104	0	68	theme	Acid	132:135	arg1	Hydrogenation					105:117	the Hydrogenation	101:117	the Hydrogenation of Levulinic Acid to γ-Valerolactone	101:154	Robust Ruthenium Catalysts Supported on Mesoporous Cyclodextrin-Templated TiO2-SiO2 Mixed Oxides for the Hydrogenation of Levulinic Acid to γ-Valerolactone.
33572104	0	69	theme	Ruthenium	7:15	arg1	Catalysts					17:25	Robust Ruthenium Catalysts	0:25	Robust Ruthenium Catalysts	0:25	Robust Ruthenium Catalysts Supported on Mesoporous Cyclodextrin-Templated TiO2-SiO2 Mixed Oxides for the Hydrogenation of Levulinic Acid to γ-Valerolactone.
33572104	8	70	theme	surface	1607:1613	arg1	morphology					1615:1624	the surface morphology	1603:1624	the surface morphology of Ru nanocrystals and pore characteristics of TiO2-based materials for catalytic applications in hydrogenation reactions	1603:1746	This work offers an effective approach to the utilization of cyclodextrins for engineering the surface morphology of Ru nanocrystals and pore characteristics of TiO2-based materials for catalytic applications in hydrogenation reactions.
33572104	1	71	theme	@	317:317	arg1	catalysts					328:336	composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts	284:336	composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts	284:336	In this paper, we present a versatile template-directed colloidal self-assembly method for the fabrication in aqueous phase of composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts.
33572104	2	72	theme	tetraethyl	481:490	arg1	assemblies					403:412	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies	339:412	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies	339:412	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	2	72	theme	tetraethyl	481:490	arg1	orthosilicate					492:504	tetraethyl orthosilicate	481:504	tetraethyl orthosilicate	481:504	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	3	73	theme	electron	739:746	arg1	scattering					614:623	dynamic light scattering	600:623	dynamic light scattering	600:623	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	3	73	theme	electron	739:746	arg1	microscopy					748:757	high-resolution transmission electron microscopy	710:757	high-resolution transmission electron microscopy	710:757	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	0	74	theme	Mesoporous	40:49	arg1	Oxides					90:95	Mesoporous Cyclodextrin-Templated TiO2-SiO2 Mixed Oxides	40:95	Mesoporous Cyclodextrin-Templated TiO2-SiO2 Mixed Oxides for the Hydrogenation of Levulinic Acid to γ-Valerolactone	40:154	Robust Ruthenium Catalysts Supported on Mesoporous Cyclodextrin-Templated TiO2-SiO2 Mixed Oxides for the Hydrogenation of Levulinic Acid to γ-Valerolactone.
33572104	1	75	theme	catalysts	328:336	arg1	phase					275:279	aqueous phase	267:279	aqueous phase of composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts	267:336	In this paper, we present a versatile template-directed colloidal self-assembly method for the fabrication in aqueous phase of composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts.
33572104	5	76	theme	catalytic	1121:1129	arg1	performance					1131:1141	Their catalytic performance	1115:1141	Their catalytic performance	1115:1141	Their catalytic performance was then evaluated in the aqueous phase hydrogenation of levulinic acid (LA) to γ-valerolactone (GVL) under mild conditions (50 °C, 50 bar H2).
33572104	4	77	theme	RuO2	1096:1099	arg1	nanocrystals					1101:1112	the RuO2 nanocrystals	1092:1112	the RuO2 nanocrystals	1092:1112	Results revealed that both the supramolecular template and the silica loading had a strong impact on the pore characteristics and crystalline structure of the mixed oxides, as well as on the morphology of the RuO2 nanocrystals.
33572104	8	78	theme	nanocrystals	1632:1643	arg1	morphology					1615:1624	the surface morphology	1603:1624	the surface morphology of Ru nanocrystals and pore characteristics of TiO2-based materials for catalytic applications in hydrogenation reactions	1603:1746	This work offers an effective approach to the utilization of cyclodextrins for engineering the surface morphology of Ru nanocrystals and pore characteristics of TiO2-based materials for catalytic applications in hydrogenation reactions.
33572104	3	79	theme	high-resolution	710:724	arg1	scattering					614:623	dynamic light scattering	600:623	dynamic light scattering	600:623	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	3	79	theme	high-resolution	710:724	arg1	microscopy					748:757	high-resolution transmission electron microscopy	710:757	high-resolution transmission electron microscopy	710:757	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	2	80	theme	building	460:467	arg1	blocks					469:474	building blocks	460:474	building blocks	460:474	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	0	81	theme	TiO2-SiO2	74:82	arg1	Oxides					90:95	Mesoporous Cyclodextrin-Templated TiO2-SiO2 Mixed Oxides	40:95	Mesoporous Cyclodextrin-Templated TiO2-SiO2 Mixed Oxides for the Hydrogenation of Levulinic Acid to γ-Valerolactone	40:154	Robust Ruthenium Catalysts Supported on Mesoporous Cyclodextrin-Templated TiO2-SiO2 Mixed Oxides for the Hydrogenation of Levulinic Acid to γ-Valerolactone.
33572104	2	82	theme	silica	511:516	arg1	assemblies					403:412	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies	339:412	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies	339:412	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	2	82	theme	silica	511:516	arg1	source					518:523	a silica source	509:523	a silica source	509:523	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	8	83	theme	pore	1649:1652	arg1	characteristics					1654:1668	pore characteristics	1649:1668	pore characteristics	1649:1668	This work offers an effective approach to the utilization of cyclodextrins for engineering the surface morphology of Ru nanocrystals and pore characteristics of TiO2-based materials for catalytic applications in hydrogenation reactions.
33572104	3	84	theme	annular	771:777	arg1	scattering					614:623	dynamic light scattering	600:623	dynamic light scattering	600:623	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	3	84	theme	annular	771:777	arg1	bright-field					779:790	high-angle annular bright-field	760:790	high-angle annular bright-field	760:790	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	4	85	theme	silica	950:955	arg1	loading					957:963	the silica loading	946:963	the silica loading	946:963	Results revealed that both the supramolecular template and the silica loading had a strong impact on the pore characteristics and crystalline structure of the mixed oxides, as well as on the morphology of the RuO2 nanocrystals.
33572104	3	86	theme	different	558:566	arg1	stages					568:573	different stages	558:573	different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping	558:884	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	3	87	dep	together	850:857	arg1	with					859:862	with	859:862	with	859:862	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	2	88	theme	F127	383:386	arg1	assemblies					403:412	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies	339:412	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies	339:412	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	2	88	theme	F127	383:386	arg1	source					518:523	a silica source	509:523	a silica source	509:523	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	2	88	theme	F127	383:386	arg1	colloids					448:455	TiO2 colloids	443:455	TiO2 colloids as building blocks	443:474	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	2	88	theme	F127	383:386	arg1	orthosilicate					492:504	tetraethyl orthosilicate	481:504	tetraethyl orthosilicate	481:504	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	2	88	theme	F127	383:386	arg1	templates					432:440	soft templates	427:440	soft templates	427:440	Randomly methylated β-cyclodextrin/Pluronic F127 supramolecular assemblies were used as soft templates, TiO2 colloids as building blocks, and tetraethyl orthosilicate as a silica source.
33572104	8	89	theme	materials	1684:1692	arg1	nanocrystals					1632:1643	Ru nanocrystals	1629:1643	Ru nanocrystals	1629:1643	This work offers an effective approach to the utilization of cyclodextrins for engineering the surface morphology of Ru nanocrystals and pore characteristics of TiO2-based materials for catalytic applications in hydrogenation reactions.
33572104	8	89	theme	materials	1684:1692	arg1	characteristics					1654:1668	pore characteristics	1649:1668	pore characteristics	1649:1668	This work offers an effective approach to the utilization of cyclodextrins for engineering the surface morphology of Ru nanocrystals and pore characteristics of TiO2-based materials for catalytic applications in hydrogenation reactions.
33572104	3	90	theme	scanning	807:814	arg1	microscopy					838:847	dark-field scanning transmission electron microscopy	796:847	dark-field scanning transmission electron microscopy	796:847	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	4	91	theme	supramolecular	918:931	arg1	template					933:940	the supramolecular template	914:940	the supramolecular template	914:940	Results revealed that both the supramolecular template and the silica loading had a strong impact on the pore characteristics and crystalline structure of the mixed oxides, as well as on the morphology of the RuO2 nanocrystals.
33572104	3	92	theme	synthesis	584:592	arg1	stages					568:573	different stages	558:573	different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping	558:884	Catalysts were characterized at different stages of their synthesis using dynamic light scattering, N2-adsorption analysis, powder X-ray diffraction, temperature programmed reduction, high-resolution transmission electron microscopy, high-angle annular bright-field and dark-field scanning transmission electron microscopy, together with EDS elemental mapping.
33572104	1	93	theme	aqueous	267:273	arg1	phase					275:279	aqueous phase	267:279	aqueous phase of composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts	267:336	In this paper, we present a versatile template-directed colloidal self-assembly method for the fabrication in aqueous phase of composition-tuned mesoporous RuO2@TiO2-SiO2 catalysts.
34441556	0	0	theme	Extraction	151:160	arg1	Technologies					162:173	Different Extraction Technologies	141:173	Different Extraction Technologies	141:173	The Chemical, Structural, and Biological Properties of Crude Polysaccharides from Sweet Tea (Lithocarpus litseifolius (Hance) Chun) Based on Different Extraction Technologies.
34441556	4	1	theme	diluted	695:701	arg1	solution					710:717	diluted alkali solution	695:717	diluted alkali solution	695:717	STPs extracted by deep-eutectic solvents and diluted alkali solution exhibited the most favorable biological properties.
34441556	1	2	theme	tea	233:235	arg1	STPs					300:303	STPs	300:303	STPs	300:303	Eight extraction technologies were used to extract sweet tea (Lithocarpus litseifolius (Hance) Chun) crude polysaccharides (STPs), and their chemical, structural, and biological properties were studied and compared.
34441556	1	2	theme	tea	233:235	arg1	polysaccharides					283:297	sweet tea (Lithocarpus litseifolius (Hance) Chun) crude polysaccharides	227:297	extract sweet tea (Lithocarpus litseifolius (Hance) Chun) crude polysaccharides (STPs)	219:304	Eight extraction technologies were used to extract sweet tea (Lithocarpus litseifolius (Hance) Chun) crude polysaccharides (STPs), and their chemical, structural, and biological properties were studied and compared.
34441556	0	3	theme	Polysaccharides	61:75	arg1	Properties					41:50	The Chemical, Structural, and Biological Properties	0:50	The Chemical, Structural, and Biological Properties of Crude Polysaccharides from Sweet Tea (Lithocarpus litseifolius (Hance) Chun)	0:130	The Chemical, Structural, and Biological Properties of Crude Polysaccharides from Sweet Tea (Lithocarpus litseifolius (Hance) Chun) Based on Different Extraction Technologies.
34441556	0	4	theme	Different	141:149	arg1	Technologies					162:173	Different Extraction Technologies	141:173	Different Extraction Technologies	141:173	The Chemical, Structural, and Biological Properties of Crude Polysaccharides from Sweet Tea (Lithocarpus litseifolius (Hance) Chun) Based on Different Extraction Technologies.
34441556	6	5	theme	STPs	1113:1116	arg1	properties					1099:1108	the chemical, structural, and biological properties	1058:1108	the chemical, structural, and biological properties of STPs	1058:1116	To the best of our knowledge, this study reports the chemical, structural, and biological properties of STPs, and the results contribute to understanding the relationship between the chemical composition and biological properties of STPs.
34441556	2	6	dep	compositions	418:429	arg1	the					414:416	the	414:416	the	414:416	Results revealed that the compositions, structures, and biological properties of STPs varied dependent on different extraction technologies.
34441556	6	7	theme	chemical	1192:1199	arg1	composition					1201:1211	chemical composition	1192:1211	chemical composition	1192:1211	To the best of our knowledge, this study reports the chemical, structural, and biological properties of STPs, and the results contribute to understanding the relationship between the chemical composition and biological properties of STPs.
34441556	3	8	theme	alkali	633:638	arg1	solution					640:647	diluted alkali solution	625:647	diluted alkali solution	625:647	Protein-bound polysaccharides and some hemicellulose could be extracted from sweet tea with diluted alkali solution.
34441556	5	9	from	presence	908:915	arg1	STPs					942:945	STPs	942:945	STPs	942:945	Moreover, according to the heat map, total phenolic content was most strongly correlated with biological properties, indicating that the presence of phenolic compounds in STPs might be the main contributor to their biological properties.
34441556	0	10	dep	Lithocarpus	93:103	arg1	litseifolius					105:116	Lithocarpus litseifolius	93:116	Lithocarpus litseifolius (Hance) Chun	93:129	The Chemical, Structural, and Biological Properties of Crude Polysaccharides from Sweet Tea (Lithocarpus litseifolius (Hance) Chun) Based on Different Extraction Technologies.
34441556	4	11	theme	biological	748:757	arg1	properties					759:768	the most favorable biological properties	729:768	the most favorable biological properties	729:768	STPs extracted by deep-eutectic solvents and diluted alkali solution exhibited the most favorable biological properties.
34441556	0	12	theme	Sweet	82:86	arg1	Tea					88:90	Sweet Tea	82:90	Sweet Tea (Lithocarpus litseifolius (Hance) Chun)	82:130	The Chemical, Structural, and Biological Properties of Crude Polysaccharides from Sweet Tea (Lithocarpus litseifolius (Hance) Chun) Based on Different Extraction Technologies.
34441556	0	13	from	Properties	41:50	arg1	Tea					88:90	Sweet Tea	82:90	Sweet Tea (Lithocarpus litseifolius (Hance) Chun)	82:130	The Chemical, Structural, and Biological Properties of Crude Polysaccharides from Sweet Tea (Lithocarpus litseifolius (Hance) Chun) Based on Different Extraction Technologies.
34441556	2	14	theme	STPs	473:476	arg1	compositions					418:429	compositions	418:429	compositions	418:429	Results revealed that the compositions, structures, and biological properties of STPs varied dependent on different extraction technologies.
34441556	2	14	theme	STPs	473:476	arg1	properties					459:468	biological properties	448:468	biological properties	448:468	Results revealed that the compositions, structures, and biological properties of STPs varied dependent on different extraction technologies.
34441556	2	14	theme	STPs	473:476	arg1	structures					432:441	structures	432:441	structures	432:441	Results revealed that the compositions, structures, and biological properties of STPs varied dependent on different extraction technologies.
34441556	1	15	theme	chemical	317:324	arg1	properties					354:363	their chemical, structural, and biological properties	311:363	their chemical, structural, and biological properties	311:363	Eight extraction technologies were used to extract sweet tea (Lithocarpus litseifolius (Hance) Chun) crude polysaccharides (STPs), and their chemical, structural, and biological properties were studied and compared.
34441556	0	16	dep	Tea	88:90	arg1	Hance					119:123	Hance	119:123	Hance	119:123	The Chemical, Structural, and Biological Properties of Crude Polysaccharides from Sweet Tea (Lithocarpus litseifolius (Hance) Chun) Based on Different Extraction Technologies.
34441556	0	16	dep	Tea	88:90	arg1	Chun					126:129	Chun	126:129	Chun	126:129	The Chemical, Structural, and Biological Properties of Crude Polysaccharides from Sweet Tea (Lithocarpus litseifolius (Hance) Chun) Based on Different Extraction Technologies.
34441556	0	16	dep	Tea	88:90	arg1	Lithocarpus					93:103	Lithocarpus	93:103	Lithocarpus	93:103	The Chemical, Structural, and Biological Properties of Crude Polysaccharides from Sweet Tea (Lithocarpus litseifolius (Hance) Chun) Based on Different Extraction Technologies.
34441556	5	17	attach	presence	908:915	arg1	STPs					942:945	STPs	942:945	STPs	942:945	Moreover, according to the heat map, total phenolic content was most strongly correlated with biological properties, indicating that the presence of phenolic compounds in STPs might be the main contributor to their biological properties.
34441556	5	17	attach	presence	908:915	arg2	compounds					929:937	phenolic compounds	920:937	phenolic compounds	920:937	Moreover, according to the heat map, total phenolic content was most strongly correlated with biological properties, indicating that the presence of phenolic compounds in STPs might be the main contributor to their biological properties.
34441556	6	18	theme	structural	1072:1081	arg1	properties					1099:1108	the chemical, structural, and biological properties	1058:1108	the chemical, structural, and biological properties of STPs	1058:1116	To the best of our knowledge, this study reports the chemical, structural, and biological properties of STPs, and the results contribute to understanding the relationship between the chemical composition and biological properties of STPs.
34441556	5	19	theme	biological	865:874	arg1	properties					876:885	biological properties	865:885	biological properties	865:885	Moreover, according to the heat map, total phenolic content was most strongly correlated with biological properties, indicating that the presence of phenolic compounds in STPs might be the main contributor to their biological properties.
34441556	0	20	theme	Chemical	4:11	arg1	Properties					41:50	The Chemical, Structural, and Biological Properties	0:50	The Chemical, Structural, and Biological Properties of Crude Polysaccharides from Sweet Tea (Lithocarpus litseifolius (Hance) Chun)	0:130	The Chemical, Structural, and Biological Properties of Crude Polysaccharides from Sweet Tea (Lithocarpus litseifolius (Hance) Chun) Based on Different Extraction Technologies.
34441556	1	21	theme	structural	327:336	arg1	properties					354:363	their chemical, structural, and biological properties	311:363	their chemical, structural, and biological properties	311:363	Eight extraction technologies were used to extract sweet tea (Lithocarpus litseifolius (Hance) Chun) crude polysaccharides (STPs), and their chemical, structural, and biological properties were studied and compared.
34441556	3	22	with	tea	616:618	arg1	solution					640:647	diluted alkali solution	625:647	diluted alkali solution	625:647	Protein-bound polysaccharides and some hemicellulose could be extracted from sweet tea with diluted alkali solution.
34441556	4	23	theme	favorable	738:746	arg1	properties					759:768	the most favorable biological properties	729:768	the most favorable biological properties	729:768	STPs extracted by deep-eutectic solvents and diluted alkali solution exhibited the most favorable biological properties.
34441556	5	24	theme	total	808:812	arg1	content					823:829	total phenolic content	808:829	total phenolic content	808:829	Moreover, according to the heat map, total phenolic content was most strongly correlated with biological properties, indicating that the presence of phenolic compounds in STPs might be the main contributor to their biological properties.
34441556	6	25	theme	STPs	1242:1245	arg1	composition					1201:1211	chemical composition	1192:1211	chemical composition	1192:1211	To the best of our knowledge, this study reports the chemical, structural, and biological properties of STPs, and the results contribute to understanding the relationship between the chemical composition and biological properties of STPs.
34441556	6	25	theme	STPs	1242:1245	arg1	properties					1228:1237	biological properties	1217:1237	biological properties	1217:1237	To the best of our knowledge, this study reports the chemical, structural, and biological properties of STPs, and the results contribute to understanding the relationship between the chemical composition and biological properties of STPs.
34441556	0	26	theme	Structural	14:23	arg1	Properties					41:50	The Chemical, Structural, and Biological Properties	0:50	The Chemical, Structural, and Biological Properties of Crude Polysaccharides from Sweet Tea (Lithocarpus litseifolius (Hance) Chun)	0:130	The Chemical, Structural, and Biological Properties of Crude Polysaccharides from Sweet Tea (Lithocarpus litseifolius (Hance) Chun) Based on Different Extraction Technologies.
34441556	2	27	theme	extraction	508:517	arg1	technologies					519:530	different extraction technologies	498:530	different extraction technologies	498:530	Results revealed that the compositions, structures, and biological properties of STPs varied dependent on different extraction technologies.
34441556	1	28	dep	extract	219:225	arg1	STPs					300:303	STPs	300:303	STPs	300:303	Eight extraction technologies were used to extract sweet tea (Lithocarpus litseifolius (Hance) Chun) crude polysaccharides (STPs), and their chemical, structural, and biological properties were studied and compared.
34441556	1	28	dep	extract	219:225	arg1	polysaccharides					283:297	sweet tea (Lithocarpus litseifolius (Hance) Chun) crude polysaccharides	227:297	extract sweet tea (Lithocarpus litseifolius (Hance) Chun) crude polysaccharides (STPs)	219:304	Eight extraction technologies were used to extract sweet tea (Lithocarpus litseifolius (Hance) Chun) crude polysaccharides (STPs), and their chemical, structural, and biological properties were studied and compared.
34441556	3	29	theme	Protein-bound	533:545	arg1	polysaccharides					547:561	Protein-bound polysaccharides	533:561	Protein-bound polysaccharides	533:561	Protein-bound polysaccharides and some hemicellulose could be extracted from sweet tea with diluted alkali solution.
34441556	1	30	theme	extraction	182:191	arg1	technologies					193:204	Eight extraction technologies	176:204	Eight extraction technologies	176:204	Eight extraction technologies were used to extract sweet tea (Lithocarpus litseifolius (Hance) Chun) crude polysaccharides (STPs), and their chemical, structural, and biological properties were studied and compared.
34441556	5	31	theme	phenolic	920:927	arg1	compounds					929:937	phenolic compounds	920:937	phenolic compounds	920:937	Moreover, according to the heat map, total phenolic content was most strongly correlated with biological properties, indicating that the presence of phenolic compounds in STPs might be the main contributor to their biological properties.
34441556	2	32	theme	different	498:506	arg1	technologies					519:530	different extraction technologies	498:530	different extraction technologies	498:530	Results revealed that the compositions, structures, and biological properties of STPs varied dependent on different extraction technologies.
34441556	1	33	theme	biological	343:352	arg1	properties					354:363	their chemical, structural, and biological properties	311:363	their chemical, structural, and biological properties	311:363	Eight extraction technologies were used to extract sweet tea (Lithocarpus litseifolius (Hance) Chun) crude polysaccharides (STPs), and their chemical, structural, and biological properties were studied and compared.
34441556	5	34	theme	phenolic	814:821	arg1	content					823:829	total phenolic content	808:829	total phenolic content	808:829	Moreover, according to the heat map, total phenolic content was most strongly correlated with biological properties, indicating that the presence of phenolic compounds in STPs might be the main contributor to their biological properties.
34441556	5	35	theme	heat	798:801	arg1	map					803:805	the heat map	794:805	the heat map	794:805	Moreover, according to the heat map, total phenolic content was most strongly correlated with biological properties, indicating that the presence of phenolic compounds in STPs might be the main contributor to their biological properties.
34441556	1	36	theme	crude	277:281	arg1	STPs					300:303	STPs	300:303	STPs	300:303	Eight extraction technologies were used to extract sweet tea (Lithocarpus litseifolius (Hance) Chun) crude polysaccharides (STPs), and their chemical, structural, and biological properties were studied and compared.
34441556	1	36	theme	crude	277:281	arg1	polysaccharides					283:297	sweet tea (Lithocarpus litseifolius (Hance) Chun) crude polysaccharides	227:297	extract sweet tea (Lithocarpus litseifolius (Hance) Chun) crude polysaccharides (STPs)	219:304	Eight extraction technologies were used to extract sweet tea (Lithocarpus litseifolius (Hance) Chun) crude polysaccharides (STPs), and their chemical, structural, and biological properties were studied and compared.
34441556	3	37	theme	diluted	625:631	arg1	solution					640:647	diluted alkali solution	625:647	diluted alkali solution	625:647	Protein-bound polysaccharides and some hemicellulose could be extracted from sweet tea with diluted alkali solution.
34441556	6	38	theme	biological	1217:1226	arg1	properties					1228:1237	biological properties	1217:1237	biological properties	1217:1237	To the best of our knowledge, this study reports the chemical, structural, and biological properties of STPs, and the results contribute to understanding the relationship between the chemical composition and biological properties of STPs.
34441556	1	39	dep	tea	233:235	arg1	Chun					271:274	Chun	271:274	Chun	271:274	Eight extraction technologies were used to extract sweet tea (Lithocarpus litseifolius (Hance) Chun) crude polysaccharides (STPs), and their chemical, structural, and biological properties were studied and compared.
34441556	1	39	dep	tea	233:235	arg1	Lithocarpus					238:248	Lithocarpus	238:248	Lithocarpus	238:248	Eight extraction technologies were used to extract sweet tea (Lithocarpus litseifolius (Hance) Chun) crude polysaccharides (STPs), and their chemical, structural, and biological properties were studied and compared.
34441556	1	39	dep	tea	233:235	arg1	Hance					264:268	Hance	264:268	Hance	264:268	Eight extraction technologies were used to extract sweet tea (Lithocarpus litseifolius (Hance) Chun) crude polysaccharides (STPs), and their chemical, structural, and biological properties were studied and compared.
34441556	0	40	theme	Biological	30:39	arg1	Properties					41:50	The Chemical, Structural, and Biological Properties	0:50	The Chemical, Structural, and Biological Properties of Crude Polysaccharides from Sweet Tea (Lithocarpus litseifolius (Hance) Chun)	0:130	The Chemical, Structural, and Biological Properties of Crude Polysaccharides from Sweet Tea (Lithocarpus litseifolius (Hance) Chun) Based on Different Extraction Technologies.
34441556	1	41	dep	Lithocarpus	238:248	arg1	litseifolius					250:261	Lithocarpus litseifolius	238:261	Lithocarpus litseifolius (Hance) Chun	238:274	Eight extraction technologies were used to extract sweet tea (Lithocarpus litseifolius (Hance) Chun) crude polysaccharides (STPs), and their chemical, structural, and biological properties were studied and compared.
34441556	4	42	theme	alkali	703:708	arg1	solution					710:717	diluted alkali solution	695:717	diluted alkali solution	695:717	STPs extracted by deep-eutectic solvents and diluted alkali solution exhibited the most favorable biological properties.
34441556	5	43	theme	biological	986:995	arg1	properties					997:1006	their biological properties	980:1006	their biological properties	980:1006	Moreover, according to the heat map, total phenolic content was most strongly correlated with biological properties, indicating that the presence of phenolic compounds in STPs might be the main contributor to their biological properties.
34441556	3	44	theme	sweet	610:614	arg1	tea					616:618	sweet tea	610:618	sweet tea with diluted alkali solution	610:647	Protein-bound polysaccharides and some hemicellulose could be extracted from sweet tea with diluted alkali solution.
34441556	5	45	theme	compounds	929:937	arg1	contributor					965:975	the main contributor	956:975	the main contributor to their biological properties	956:1006	Moreover, according to the heat map, total phenolic content was most strongly correlated with biological properties, indicating that the presence of phenolic compounds in STPs might be the main contributor to their biological properties.
34441556	5	45	theme	compounds	929:937	arg1	presence					908:915	the presence	904:915	the presence of phenolic compounds in STPs	904:945	Moreover, according to the heat map, total phenolic content was most strongly correlated with biological properties, indicating that the presence of phenolic compounds in STPs might be the main contributor to their biological properties.
34441556	0	46	from	Tea	88:90	arg1	Polysaccharides					61:75	Crude Polysaccharides	55:75	Crude Polysaccharides from Sweet Tea (Lithocarpus litseifolius (Hance) Chun)	55:130	The Chemical, Structural, and Biological Properties of Crude Polysaccharides from Sweet Tea (Lithocarpus litseifolius (Hance) Chun) Based on Different Extraction Technologies.
34441556	0	46	from	Tea	88:90	arg1	Properties					41:50	The Chemical, Structural, and Biological Properties	0:50	The Chemical, Structural, and Biological Properties of Crude Polysaccharides from Sweet Tea (Lithocarpus litseifolius (Hance) Chun)	0:130	The Chemical, Structural, and Biological Properties of Crude Polysaccharides from Sweet Tea (Lithocarpus litseifolius (Hance) Chun) Based on Different Extraction Technologies.
34441556	5	47	theme	main	960:963	arg1	contributor					965:975	the main contributor	956:975	the main contributor to their biological properties	956:1006	Moreover, according to the heat map, total phenolic content was most strongly correlated with biological properties, indicating that the presence of phenolic compounds in STPs might be the main contributor to their biological properties.
34441556	5	47	theme	main	960:963	arg1	presence					908:915	the presence	904:915	the presence of phenolic compounds in STPs	904:945	Moreover, according to the heat map, total phenolic content was most strongly correlated with biological properties, indicating that the presence of phenolic compounds in STPs might be the main contributor to their biological properties.
34441556	6	48	dep	composition	1201:1211	arg1	the					1188:1190	the	1188:1190	the	1188:1190	To the best of our knowledge, this study reports the chemical, structural, and biological properties of STPs, and the results contribute to understanding the relationship between the chemical composition and biological properties of STPs.
34441556	0	49	theme	Crude	55:59	arg1	Polysaccharides					61:75	Crude Polysaccharides	55:75	Crude Polysaccharides from Sweet Tea (Lithocarpus litseifolius (Hance) Chun)	55:130	The Chemical, Structural, and Biological Properties of Crude Polysaccharides from Sweet Tea (Lithocarpus litseifolius (Hance) Chun) Based on Different Extraction Technologies.
34441556	1	50	used	used	211:214	arg2	technologies					193:204	Eight extraction technologies	176:204	Eight extraction technologies	176:204	Eight extraction technologies were used to extract sweet tea (Lithocarpus litseifolius (Hance) Chun) crude polysaccharides (STPs), and their chemical, structural, and biological properties were studied and compared.
34441556	6	51	theme	biological	1088:1097	arg1	properties					1099:1108	the chemical, structural, and biological properties	1058:1108	the chemical, structural, and biological properties of STPs	1058:1116	To the best of our knowledge, this study reports the chemical, structural, and biological properties of STPs, and the results contribute to understanding the relationship between the chemical composition and biological properties of STPs.
34441556	6	52	theme	chemical	1062:1069	arg1	properties					1099:1108	the chemical, structural, and biological properties	1058:1108	the chemical, structural, and biological properties of STPs	1058:1116	To the best of our knowledge, this study reports the chemical, structural, and biological properties of STPs, and the results contribute to understanding the relationship between the chemical composition and biological properties of STPs.
34441556	2	53	theme	biological	448:457	arg1	properties					459:468	biological properties	448:468	biological properties	448:468	Results revealed that the compositions, structures, and biological properties of STPs varied dependent on different extraction technologies.
34441556	4	54	theme	deep-eutectic	668:680	arg1	solvents					682:689	deep-eutectic solvents	668:689	deep-eutectic solvents	668:689	STPs extracted by deep-eutectic solvents and diluted alkali solution exhibited the most favorable biological properties.
34441556	1	55	theme	sweet	227:231	arg1	tea					233:235	sweet tea	227:235	extract sweet tea (Lithocarpus litseifolius (Hance) Chun) crude polysaccharides (STPs)	219:304	Eight extraction technologies were used to extract sweet tea (Lithocarpus litseifolius (Hance) Chun) crude polysaccharides (STPs), and their chemical, structural, and biological properties were studied and compared.
32061059	6	0	theme	humid	990:994	arg1	loss					1001:1004	the significant humid mass loss	974:1004	the significant humid mass loss	974:1004	Following a 28-day incubation in water, cross-linked CH scaffold had no changes on humid mass, and CH-Ca featured a controlled degradability profile since the significant humid mass loss was observed only after 21 (26.0%) and 28 days (42.2%).
32061059	4	1	theme	porous	655:660	arg1	CH-Ca					696:700	CH-Ca	696:700	CH-Ca	696:700	Scanning electron microscopy/energy-dispersive spectroscopy demonstrated the fabrication of a highly porous calcium-linked chitosan scaffold (CH-Ca), with a well-organized and interconnected porous network.
32061059	4	1	theme	porous	655:660	arg1	scaffold					686:693	a highly porous calcium-linked chitosan scaffold	646:693	a highly porous calcium-linked chitosan scaffold (CH-Ca)	646:701	Scanning electron microscopy/energy-dispersive spectroscopy demonstrated the fabrication of a highly porous calcium-linked chitosan scaffold (CH-Ca), with a well-organized and interconnected porous network.
32061059	2	2	theme	porosity	351:358	arg1	degree					341:346	the degree	337:346	the degree of porosity and chemical composition of chitosan scaffolds	337:405	A calcium hydroxide (Ca[OH]2 ) suspension was used to modulate the degree of porosity and chemical composition of chitosan scaffolds.
32061059	8	3	theme	Cross-linked	1215:1226	arg1	scaffold					1234:1241	Cross-linked CH-Ca scaffold	1215:1241	Cross-linked CH-Ca scaffold	1215:1241	Cross-linked CH-Ca scaffold featured a sustained Ca2+ release up to 21 days in a humid environment.
32061059	4	4	theme	chitosan	677:684	arg1	CH-Ca					696:700	CH-Ca	696:700	CH-Ca	696:700	Scanning electron microscopy/energy-dispersive spectroscopy demonstrated the fabrication of a highly porous calcium-linked chitosan scaffold (CH-Ca), with a well-organized and interconnected porous network.
32061059	4	4	theme	chitosan	677:684	arg1	scaffold					686:693	a highly porous calcium-linked chitosan scaffold	646:693	a highly porous calcium-linked chitosan scaffold (CH-Ca)	646:701	Scanning electron microscopy/energy-dispersive spectroscopy demonstrated the fabrication of a highly porous calcium-linked chitosan scaffold (CH-Ca), with a well-organized and interconnected porous network.
32061059	10	5	theme	matrix	1754:1759	arg1	deposition					1761:1770	calcium-rich matrix deposition	1741:1770	calcium-rich matrix deposition	1741:1770	HDPCs seeded onto CH-Ca featured significantly higher ALP activity, and gene expressions for ALP, Col1, DMP-1, and DSPP in comparison to CH, leading to a significant 3.5 times increase in calcium-rich matrix deposition.
32061059	4	6	theme	interconnected	730:743	arg1	network					752:758	a well-organized and interconnected porous network	709:758	a well-organized and interconnected porous network	709:758	Scanning electron microscopy/energy-dispersive spectroscopy demonstrated the fabrication of a highly porous calcium-linked chitosan scaffold (CH-Ca), with a well-organized and interconnected porous network.
32061059	10	7	theme	higher	1600:1605	arg1	activity					1611:1618	significantly higher ALP activity	1586:1618	significantly higher ALP activity	1586:1618	HDPCs seeded onto CH-Ca featured significantly higher ALP activity, and gene expressions for ALP, Col1, DMP-1, and DSPP in comparison to CH, leading to a significant 3.5 times increase in calcium-rich matrix deposition.
32061059	7	8	theme	cross-linked	1148:1159	arg1	scaffolds					1161:1169	cross-linked scaffolds	1148:1169	cross-linked scaffolds	1148:1169	Fourier-transform infrared spectroscopy indicated the establishment of Schiff base on cross-linked scaffolds, along with calcium complexation for CH-Ca.
32061059	6	9	theme	CH	872:873	arg1	scaffold					875:882	cross-linked CH scaffold	859:882	cross-linked CH scaffold	859:882	Following a 28-day incubation in water, cross-linked CH scaffold had no changes on humid mass, and CH-Ca featured a controlled degradability profile since the significant humid mass loss was observed only after 21 (26.0%) and 28 days (42.2%).
32061059	2	10	theme	Ca[OH	295:299	arg1	suspension					305:314	A calcium hydroxide (Ca[OH]2 ) suspension	274:314	A calcium hydroxide (Ca[OH]2 ) suspension	274:314	A calcium hydroxide (Ca[OH]2 ) suspension was used to modulate the degree of porosity and chemical composition of chitosan scaffolds.
32061059	6	11	theme	controlled	935:944	arg1	profile					960:966	a controlled degradability profile	933:966	a controlled degradability profile	933:966	Following a 28-day incubation in water, cross-linked CH scaffold had no changes on humid mass, and CH-Ca featured a controlled degradability profile since the significant humid mass loss was observed only after 21 (26.0%) and 28 days (42.2%).
32061059	7	12	theme	base	1140:1143	arg1	establishment					1116:1128	the establishment	1112:1128	the establishment of Schiff base on cross-linked scaffolds	1112:1169	Fourier-transform infrared spectroscopy indicated the establishment of Schiff base on cross-linked scaffolds, along with calcium complexation for CH-Ca.
32061059	1	13	theme	porous	196:201	arg1	scaffold					231:238	a highly porous calcium-containing chitosan scaffold	187:238	a highly porous calcium-containing chitosan scaffold suitable for dentin regeneration	187:271	The aim of this study was to develop a highly porous calcium-containing chitosan scaffold suitable for dentin regeneration.
32061059	8	14	theme	Ca2+	1264:1267	arg1	release					1269:1275	a sustained Ca2+ release	1252:1275	a sustained Ca2+ release up to 21 days in a humid environment	1252:1312	Cross-linked CH-Ca scaffold featured a sustained Ca2+ release up to 21 days in a humid environment.
32061059	5	15	theme	glutaraldehyde	792:805	arg1	vapor					812:816	glutaraldehyde (GA) vapor	792:816	glutaraldehyde (GA) vapor	792:816	Scaffolds were cross-linked on glutaraldehyde (GA) vapor.
32061059	10	16	theme	significant	1707:1717	arg1	increase					1729:1736	a significant 3.5 times increase	1705:1736	a significant 3.5 times increase in calcium-rich matrix deposition	1705:1770	HDPCs seeded onto CH-Ca featured significantly higher ALP activity, and gene expressions for ALP, Col1, DMP-1, and DSPP in comparison to CH, leading to a significant 3.5 times increase in calcium-rich matrix deposition.
32061059	1	17	theme	chitosan	222:229	arg1	scaffold					231:238	a highly porous calcium-containing chitosan scaffold	187:238	a highly porous calcium-containing chitosan scaffold suitable for dentin regeneration	187:271	The aim of this study was to develop a highly porous calcium-containing chitosan scaffold suitable for dentin regeneration.
32061059	3	18	theme	freezing	448:455	arg1	protocol					457:464	freezing protocol	448:464	freezing protocol	448:464	The chitosan solution concentration and freezing protocol were adjusted to optimize the porous architecture using the phase-separation technique.
32061059	4	19	theme	well-organized	711:724	arg1	network					752:758	a well-organized and interconnected porous network	709:758	a well-organized and interconnected porous network	709:758	Scanning electron microscopy/energy-dispersive spectroscopy demonstrated the fabrication of a highly porous calcium-linked chitosan scaffold (CH-Ca), with a well-organized and interconnected porous network.
32061059	1	20	theme	suitable	240:247	arg1	scaffold					231:238	a highly porous calcium-containing chitosan scaffold	187:238	a highly porous calcium-containing chitosan scaffold suitable for dentin regeneration	187:271	The aim of this study was to develop a highly porous calcium-containing chitosan scaffold suitable for dentin regeneration.
32061059	11	21	theme	CH-Ca	1807:1811	arg1	scaffolds					1813:1821	CH-Ca scaffolds	1807:1821	CH-Ca scaffolds	1807:1821	In sum, our findings suggest that CH-Ca scaffolds are attractive candidates for creating a highly porous and bioactive substrate for dentin tissue engineering.
32061059	11	21	theme	CH-Ca	1807:1811	arg1	candidates					1838:1847	attractive candidates	1827:1847	attractive candidates for creating a highly porous and bioactive substrate for dentin tissue engineering	1827:1930	In sum, our findings suggest that CH-Ca scaffolds are attractive candidates for creating a highly porous and bioactive substrate for dentin tissue engineering.
32061059	7	22	theme	Fourier-transform	1062:1078	arg1	spectroscopy					1089:1100	Fourier-transform infrared spectroscopy	1062:1100	Fourier-transform infrared spectroscopy	1062:1100	Fourier-transform infrared spectroscopy indicated the establishment of Schiff base on cross-linked scaffolds, along with calcium complexation for CH-Ca.
32061059	9	23	theme	porous	1320:1325	arg1	architecture					1338:1349	This porous and stable architecture	1315:1349	This porous and stable architecture	1315:1349	This porous and stable architecture allowed for human dental pulp cells (HDPCs) to spread throughout the scaffold, with cells exhibiting a widely stretched cytoplasm; whereas, the cells seeded onto CH scaffold were organized in clusters.
32061059	2	24	theme	hydroxide	284:292	arg1	suspension					305:314	A calcium hydroxide (Ca[OH]2 ) suspension	274:314	A calcium hydroxide (Ca[OH]2 ) suspension	274:314	A calcium hydroxide (Ca[OH]2 ) suspension was used to modulate the degree of porosity and chemical composition of chitosan scaffolds.
32061059	7	25	from	establishment	1116:1128	arg1	scaffolds					1161:1169	cross-linked scaffolds	1148:1169	cross-linked scaffolds	1148:1169	Fourier-transform infrared spectroscopy indicated the establishment of Schiff base on cross-linked scaffolds, along with calcium complexation for CH-Ca.
32061059	9	26	theme	stable	1331:1336	arg1	architecture					1338:1349	This porous and stable architecture	1315:1349	This porous and stable architecture	1315:1349	This porous and stable architecture allowed for human dental pulp cells (HDPCs) to spread throughout the scaffold, with cells exhibiting a widely stretched cytoplasm; whereas, the cells seeded onto CH scaffold were organized in clusters.
32061059	8	27	link	Cross-linked	1215:1226	arg1	scaffold					1234:1241	Cross-linked CH-Ca scaffold	1215:1241	Cross-linked CH-Ca scaffold	1215:1241	Cross-linked CH-Ca scaffold featured a sustained Ca2+ release up to 21 days in a humid environment.
32061059	1	28	theme	study	166:170	arg1	aim					154:156	The aim	150:156	The aim of this study	150:170	The aim of this study was to develop a highly porous calcium-containing chitosan scaffold suitable for dentin regeneration.
32061059	0	29	theme	potential	98:106	arg1	application					108:118	potential application	98:118	potential application in dentin tissue engineering	98:147	Characterization of novel calcium hydroxide-mediated highly porous chitosan-calcium scaffolds for potential application in dentin tissue engineering.
32061059	9	30	theme	human	1363:1367	arg1	HDPCs					1388:1392	HDPCs	1388:1392	HDPCs	1388:1392	This porous and stable architecture allowed for human dental pulp cells (HDPCs) to spread throughout the scaffold, with cells exhibiting a widely stretched cytoplasm; whereas, the cells seeded onto CH scaffold were organized in clusters.
32061059	9	30	theme	human	1363:1367	arg1	cells					1381:1385	human dental pulp cells	1363:1385	human dental pulp cells (HDPCs)	1363:1393	This porous and stable architecture allowed for human dental pulp cells (HDPCs) to spread throughout the scaffold, with cells exhibiting a widely stretched cytoplasm; whereas, the cells seeded onto CH scaffold were organized in clusters.
32061059	11	31	theme	dentin	1906:1911	arg1	engineering					1920:1930	dentin tissue engineering	1906:1930	dentin tissue engineering	1906:1930	In sum, our findings suggest that CH-Ca scaffolds are attractive candidates for creating a highly porous and bioactive substrate for dentin tissue engineering.
32061059	0	32	theme	tissue	130:135	arg1	engineering					137:147	dentin tissue engineering	123:147	dentin tissue engineering	123:147	Characterization of novel calcium hydroxide-mediated highly porous chitosan-calcium scaffolds for potential application in dentin tissue engineering.
32061059	9	33	theme	pulp	1376:1379	arg1	HDPCs					1388:1392	HDPCs	1388:1392	HDPCs	1388:1392	This porous and stable architecture allowed for human dental pulp cells (HDPCs) to spread throughout the scaffold, with cells exhibiting a widely stretched cytoplasm; whereas, the cells seeded onto CH scaffold were organized in clusters.
32061059	9	33	theme	pulp	1376:1379	arg1	cells					1381:1385	human dental pulp cells	1363:1385	human dental pulp cells (HDPCs)	1363:1393	This porous and stable architecture allowed for human dental pulp cells (HDPCs) to spread throughout the scaffold, with cells exhibiting a widely stretched cytoplasm; whereas, the cells seeded onto CH scaffold were organized in clusters.
32061059	2	34	theme	chitosan	388:395	arg1	scaffolds					397:405	chitosan scaffolds	388:405	chitosan scaffolds	388:405	A calcium hydroxide (Ca[OH]2 ) suspension was used to modulate the degree of porosity and chemical composition of chitosan scaffolds.
32061059	8	35	dep	21 days	1283:1289	arg1	up					1277:1278	up	1277:1278	up	1277:1278	Cross-linked CH-Ca scaffold featured a sustained Ca2+ release up to 21 days in a humid environment.
32061059	0	36	theme	novel	20:24	arg1	calcium					26:32	novel calcium	20:32	novel calcium	20:32	Characterization of novel calcium hydroxide-mediated highly porous chitosan-calcium scaffolds for potential application in dentin tissue engineering.
32061059	3	37	theme	solution	421:428	arg1	concentration					430:442	The chitosan solution concentration	408:442	The chitosan solution concentration	408:442	The chitosan solution concentration and freezing protocol were adjusted to optimize the porous architecture using the phase-separation technique.
32061059	6	38	link	cross-linked	859:870	arg1	scaffold					875:882	cross-linked CH scaffold	859:882	cross-linked CH scaffold	859:882	Following a 28-day incubation in water, cross-linked CH scaffold had no changes on humid mass, and CH-Ca featured a controlled degradability profile since the significant humid mass loss was observed only after 21 (26.0%) and 28 days (42.2%).
32061059	4	39	theme	electron	563:570	arg1	spectroscopy					601:612	Scanning electron microscopy/energy-dispersive spectroscopy	554:612	Scanning electron microscopy/energy-dispersive spectroscopy	554:612	Scanning electron microscopy/energy-dispersive spectroscopy demonstrated the fabrication of a highly porous calcium-linked chitosan scaffold (CH-Ca), with a well-organized and interconnected porous network.
32061059	0	40	theme	porous	60:65	arg1	scaffolds					84:92	highly porous chitosan-calcium scaffolds	53:92	highly porous chitosan-calcium scaffolds	53:92	Characterization of novel calcium hydroxide-mediated highly porous chitosan-calcium scaffolds for potential application in dentin tissue engineering.
32061059	3	41	theme	porous	496:501	arg1	architecture					503:514	the porous architecture	492:514	the porous architecture using the phase-separation technique	492:551	The chitosan solution concentration and freezing protocol were adjusted to optimize the porous architecture using the phase-separation technique.
32061059	6	42	theme	humid	902:906	arg1	mass					908:911	humid mass	902:911	humid mass	902:911	Following a 28-day incubation in water, cross-linked CH scaffold had no changes on humid mass, and CH-Ca featured a controlled degradability profile since the significant humid mass loss was observed only after 21 (26.0%) and 28 days (42.2%).
32061059	6	43	from	incubation	838:847	arg1	water					852:856	water	852:856	water	852:856	Following a 28-day incubation in water, cross-linked CH scaffold had no changes on humid mass, and CH-Ca featured a controlled degradability profile since the significant humid mass loss was observed only after 21 (26.0%) and 28 days (42.2%).
32061059	2	44	used	used	320:323	arg2	suspension					305:314	A calcium hydroxide (Ca[OH]2 ) suspension	274:314	A calcium hydroxide (Ca[OH]2 ) suspension	274:314	A calcium hydroxide (Ca[OH]2 ) suspension was used to modulate the degree of porosity and chemical composition of chitosan scaffolds.
32061059	4	45	theme	scaffold	686:693	arg1	fabrication					631:641	the fabrication	627:641	the fabrication of a highly porous calcium-linked chitosan scaffold (CH-Ca)	627:701	Scanning electron microscopy/energy-dispersive spectroscopy demonstrated the fabrication of a highly porous calcium-linked chitosan scaffold (CH-Ca), with a well-organized and interconnected porous network.
32061059	2	46	theme	composition	373:383	arg1	degree					341:346	the degree	337:346	the degree of porosity and chemical composition of chitosan scaffolds	337:405	A calcium hydroxide (Ca[OH]2 ) suspension was used to modulate the degree of porosity and chemical composition of chitosan scaffolds.
32061059	0	47	theme	chitosan-calcium	67:82	arg1	scaffolds					84:92	highly porous chitosan-calcium scaffolds	53:92	highly porous chitosan-calcium scaffolds	53:92	Characterization of novel calcium hydroxide-mediated highly porous chitosan-calcium scaffolds for potential application in dentin tissue engineering.
32061059	6	48	theme	mass	996:999	arg1	loss					1001:1004	the significant humid mass loss	974:1004	the significant humid mass loss	974:1004	Following a 28-day incubation in water, cross-linked CH scaffold had no changes on humid mass, and CH-Ca featured a controlled degradability profile since the significant humid mass loss was observed only after 21 (26.0%) and 28 days (42.2%).
32061059	3	49	theme	phase-separation	526:541	arg1	technique					543:551	the phase-separation technique	522:551	the phase-separation technique	522:551	The chitosan solution concentration and freezing protocol were adjusted to optimize the porous architecture using the phase-separation technique.
32061059	6	50	theme	significant	978:988	arg1	loss					1001:1004	the significant humid mass loss	974:1004	the significant humid mass loss	974:1004	Following a 28-day incubation in water, cross-linked CH scaffold had no changes on humid mass, and CH-Ca featured a controlled degradability profile since the significant humid mass loss was observed only after 21 (26.0%) and 28 days (42.2%).
32061059	9	51	theme	stretched	1461:1469	arg1	cytoplasm					1471:1479	a widely stretched cytoplasm	1452:1479	a widely stretched cytoplasm	1452:1479	This porous and stable architecture allowed for human dental pulp cells (HDPCs) to spread throughout the scaffold, with cells exhibiting a widely stretched cytoplasm; whereas, the cells seeded onto CH scaffold were organized in clusters.
32061059	8	52	from	21 days	1283:1289	arg1	environment					1302:1312	a humid environment	1294:1312	a humid environment	1294:1312	Cross-linked CH-Ca scaffold featured a sustained Ca2+ release up to 21 days in a humid environment.
32061059	4	53	theme	calcium-linked	662:675	arg1	CH-Ca					696:700	CH-Ca	696:700	CH-Ca	696:700	Scanning electron microscopy/energy-dispersive spectroscopy demonstrated the fabrication of a highly porous calcium-linked chitosan scaffold (CH-Ca), with a well-organized and interconnected porous network.
32061059	4	53	theme	calcium-linked	662:675	arg1	scaffold					686:693	a highly porous calcium-linked chitosan scaffold	646:693	a highly porous calcium-linked chitosan scaffold (CH-Ca)	646:701	Scanning electron microscopy/energy-dispersive spectroscopy demonstrated the fabrication of a highly porous calcium-linked chitosan scaffold (CH-Ca), with a well-organized and interconnected porous network.
32061059	10	54	theme	ALP	1607:1609	arg1	activity					1611:1618	significantly higher ALP activity	1586:1618	significantly higher ALP activity	1586:1618	HDPCs seeded onto CH-Ca featured significantly higher ALP activity, and gene expressions for ALP, Col1, DMP-1, and DSPP in comparison to CH, leading to a significant 3.5 times increase in calcium-rich matrix deposition.
32061059	10	55	theme	calcium-rich	1741:1752	arg1	deposition					1761:1770	calcium-rich matrix deposition	1741:1770	calcium-rich matrix deposition	1741:1770	HDPCs seeded onto CH-Ca featured significantly higher ALP activity, and gene expressions for ALP, Col1, DMP-1, and DSPP in comparison to CH, leading to a significant 3.5 times increase in calcium-rich matrix deposition.
32061059	8	56	theme	CH-Ca	1228:1232	arg1	scaffold					1234:1241	Cross-linked CH-Ca scaffold	1215:1241	Cross-linked CH-Ca scaffold	1215:1241	Cross-linked CH-Ca scaffold featured a sustained Ca2+ release up to 21 days in a humid environment.
32061059	6	57	theme	degradability	946:958	arg1	profile					960:966	a controlled degradability profile	933:966	a controlled degradability profile	933:966	Following a 28-day incubation in water, cross-linked CH scaffold had no changes on humid mass, and CH-Ca featured a controlled degradability profile since the significant humid mass loss was observed only after 21 (26.0%) and 28 days (42.2%).
32061059	7	58	theme	Schiff	1133:1138	arg1	base					1140:1143	Schiff base	1133:1143	Schiff base	1133:1143	Fourier-transform infrared spectroscopy indicated the establishment of Schiff base on cross-linked scaffolds, along with calcium complexation for CH-Ca.
32061059	6	59	theme	cross-linked	859:870	arg1	scaffold					875:882	cross-linked CH scaffold	859:882	cross-linked CH scaffold	859:882	Following a 28-day incubation in water, cross-linked CH scaffold had no changes on humid mass, and CH-Ca featured a controlled degradability profile since the significant humid mass loss was observed only after 21 (26.0%) and 28 days (42.2%).
32061059	10	60	from	increase	1729:1736	arg1	deposition					1761:1770	calcium-rich matrix deposition	1741:1770	calcium-rich matrix deposition	1741:1770	HDPCs seeded onto CH-Ca featured significantly higher ALP activity, and gene expressions for ALP, Col1, DMP-1, and DSPP in comparison to CH, leading to a significant 3.5 times increase in calcium-rich matrix deposition.
32061059	8	61	theme	humid	1296:1300	arg1	environment					1302:1312	a humid environment	1294:1312	a humid environment	1294:1312	Cross-linked CH-Ca scaffold featured a sustained Ca2+ release up to 21 days in a humid environment.
32061059	10	62	theme	gene	1625:1628	arg1	expressions					1630:1640	gene expressions	1625:1640	gene expressions for ALP, Col1, DMP-1, and DSPP	1625:1671	HDPCs seeded onto CH-Ca featured significantly higher ALP activity, and gene expressions for ALP, Col1, DMP-1, and DSPP in comparison to CH, leading to a significant 3.5 times increase in calcium-rich matrix deposition.
32061059	1	63	theme	calcium-containing	203:220	arg1	scaffold					231:238	a highly porous calcium-containing chitosan scaffold	187:238	a highly porous calcium-containing chitosan scaffold suitable for dentin regeneration	187:271	The aim of this study was to develop a highly porous calcium-containing chitosan scaffold suitable for dentin regeneration.
32061059	8	64	theme	sustained	1254:1262	arg1	release					1269:1275	a sustained Ca2+ release	1252:1275	a sustained Ca2+ release up to 21 days in a humid environment	1252:1312	Cross-linked CH-Ca scaffold featured a sustained Ca2+ release up to 21 days in a humid environment.
32061059	0	65	from	application	108:118	arg1	engineering					137:147	dentin tissue engineering	123:147	dentin tissue engineering	123:147	Characterization of novel calcium hydroxide-mediated highly porous chitosan-calcium scaffolds for potential application in dentin tissue engineering.
32061059	4	66	theme	porous	745:750	arg1	network					752:758	a well-organized and interconnected porous network	709:758	a well-organized and interconnected porous network	709:758	Scanning electron microscopy/energy-dispersive spectroscopy demonstrated the fabrication of a highly porous calcium-linked chitosan scaffold (CH-Ca), with a well-organized and interconnected porous network.
32061059	11	67	theme	attractive	1827:1836	arg1	scaffolds					1813:1821	CH-Ca scaffolds	1807:1821	CH-Ca scaffolds	1807:1821	In sum, our findings suggest that CH-Ca scaffolds are attractive candidates for creating a highly porous and bioactive substrate for dentin tissue engineering.
32061059	11	67	theme	attractive	1827:1836	arg1	candidates					1838:1847	attractive candidates	1827:1847	attractive candidates for creating a highly porous and bioactive substrate for dentin tissue engineering	1827:1930	In sum, our findings suggest that CH-Ca scaffolds are attractive candidates for creating a highly porous and bioactive substrate for dentin tissue engineering.
32061059	7	68	theme	infrared	1080:1087	arg1	spectroscopy					1089:1100	Fourier-transform infrared spectroscopy	1062:1100	Fourier-transform infrared spectroscopy	1062:1100	Fourier-transform infrared spectroscopy indicated the establishment of Schiff base on cross-linked scaffolds, along with calcium complexation for CH-Ca.
32061059	2	69	dep	suspension	305:314	arg1	2					301:301	2	301:301	2	301:301	A calcium hydroxide (Ca[OH]2 ) suspension was used to modulate the degree of porosity and chemical composition of chitosan scaffolds.
32061059	2	70	theme	calcium	276:282	arg1	suspension					305:314	A calcium hydroxide (Ca[OH]2 ) suspension	274:314	A calcium hydroxide (Ca[OH]2 ) suspension	274:314	A calcium hydroxide (Ca[OH]2 ) suspension was used to modulate the degree of porosity and chemical composition of chitosan scaffolds.
32061059	6	71	contain	had	884:886	arg2	changes					891:897	no changes	888:897	no changes	888:897	Following a 28-day incubation in water, cross-linked CH scaffold had no changes on humid mass, and CH-Ca featured a controlled degradability profile since the significant humid mass loss was observed only after 21 (26.0%) and 28 days (42.2%).
32061059	6	71	contain	had	884:886	arg1	scaffold					875:882	cross-linked CH scaffold	859:882	cross-linked CH scaffold	859:882	Following a 28-day incubation in water, cross-linked CH scaffold had no changes on humid mass, and CH-Ca featured a controlled degradability profile since the significant humid mass loss was observed only after 21 (26.0%) and 28 days (42.2%).
32061059	11	72	theme	porous	1871:1876	arg1	substrate					1892:1900	a highly porous and bioactive substrate	1862:1900	a highly porous and bioactive substrate for dentin tissue engineering	1862:1930	In sum, our findings suggest that CH-Ca scaffolds are attractive candidates for creating a highly porous and bioactive substrate for dentin tissue engineering.
32061059	4	73	theme	Scanning	554:561	arg1	spectroscopy					601:612	Scanning electron microscopy/energy-dispersive spectroscopy	554:612	Scanning electron microscopy/energy-dispersive spectroscopy	554:612	Scanning electron microscopy/energy-dispersive spectroscopy demonstrated the fabrication of a highly porous calcium-linked chitosan scaffold (CH-Ca), with a well-organized and interconnected porous network.
32061059	0	74	theme	dentin	123:128	arg1	engineering					137:147	dentin tissue engineering	123:147	dentin tissue engineering	123:147	Characterization of novel calcium hydroxide-mediated highly porous chitosan-calcium scaffolds for potential application in dentin tissue engineering.
32061059	10	75	dep	significant	1707:1717	arg1	times					1723:1727	3.5 times	1719:1727	3.5 times	1719:1727	HDPCs seeded onto CH-Ca featured significantly higher ALP activity, and gene expressions for ALP, Col1, DMP-1, and DSPP in comparison to CH, leading to a significant 3.5 times increase in calcium-rich matrix deposition.
32061059	9	76	theme	dental	1369:1374	arg1	HDPCs					1388:1392	HDPCs	1388:1392	HDPCs	1388:1392	This porous and stable architecture allowed for human dental pulp cells (HDPCs) to spread throughout the scaffold, with cells exhibiting a widely stretched cytoplasm; whereas, the cells seeded onto CH scaffold were organized in clusters.
32061059	9	76	theme	dental	1369:1374	arg1	cells					1381:1385	human dental pulp cells	1363:1385	human dental pulp cells (HDPCs)	1363:1393	This porous and stable architecture allowed for human dental pulp cells (HDPCs) to spread throughout the scaffold, with cells exhibiting a widely stretched cytoplasm; whereas, the cells seeded onto CH scaffold were organized in clusters.
32061059	2	77	theme	scaffolds	397:405	arg1	porosity					351:358	porosity	351:358	porosity	351:358	A calcium hydroxide (Ca[OH]2 ) suspension was used to modulate the degree of porosity and chemical composition of chitosan scaffolds.
32061059	2	77	theme	scaffolds	397:405	arg1	composition					373:383	chemical composition	364:383	chemical composition	364:383	A calcium hydroxide (Ca[OH]2 ) suspension was used to modulate the degree of porosity and chemical composition of chitosan scaffolds.
32061059	7	78	link	cross-linked	1148:1159	arg1	scaffolds					1161:1169	cross-linked scaffolds	1148:1169	cross-linked scaffolds	1148:1169	Fourier-transform infrared spectroscopy indicated the establishment of Schiff base on cross-linked scaffolds, along with calcium complexation for CH-Ca.
32061059	0	79	theme	calcium	26:32	arg1	Characterization					0:15	Characterization	0:15	Characterization of novel calcium	0:32	Characterization of novel calcium hydroxide-mediated highly porous chitosan-calcium scaffolds for potential application in dentin tissue engineering.
32061059	5	80	attach	cross-linked	776:787	arg3	vapor					812:816	glutaraldehyde (GA) vapor	792:816	glutaraldehyde (GA) vapor	792:816	Scaffolds were cross-linked on glutaraldehyde (GA) vapor.
32061059	4	81	theme	microscopy/energy-dispersive	572:599	arg1	spectroscopy					601:612	Scanning electron microscopy/energy-dispersive spectroscopy	554:612	Scanning electron microscopy/energy-dispersive spectroscopy	554:612	Scanning electron microscopy/energy-dispersive spectroscopy demonstrated the fabrication of a highly porous calcium-linked chitosan scaffold (CH-Ca), with a well-organized and interconnected porous network.
32061059	7	82	theme	calcium	1183:1189	arg1	complexation					1191:1202	calcium complexation	1183:1202	calcium complexation for CH-Ca	1183:1212	Fourier-transform infrared spectroscopy indicated the establishment of Schiff base on cross-linked scaffolds, along with calcium complexation for CH-Ca.
32061059	3	83	theme	chitosan	412:419	arg1	concentration					430:442	The chitosan solution concentration	408:442	The chitosan solution concentration	408:442	The chitosan solution concentration and freezing protocol were adjusted to optimize the porous architecture using the phase-separation technique.
32061059	6	84	theme	28-day	831:836	arg1	incubation					838:847	a 28-day incubation	829:847	a 28-day incubation in water	829:856	Following a 28-day incubation in water, cross-linked CH scaffold had no changes on humid mass, and CH-Ca featured a controlled degradability profile since the significant humid mass loss was observed only after 21 (26.0%) and 28 days (42.2%).
32061059	4	85	link	calcium-linked	662:675	arg1	CH-Ca					696:700	CH-Ca	696:700	CH-Ca	696:700	Scanning electron microscopy/energy-dispersive spectroscopy demonstrated the fabrication of a highly porous calcium-linked chitosan scaffold (CH-Ca), with a well-organized and interconnected porous network.
32061059	4	85	link	calcium-linked	662:675	arg1	scaffold					686:693	a highly porous calcium-linked chitosan scaffold	646:693	a highly porous calcium-linked chitosan scaffold (CH-Ca)	646:701	Scanning electron microscopy/energy-dispersive spectroscopy demonstrated the fabrication of a highly porous calcium-linked chitosan scaffold (CH-Ca), with a well-organized and interconnected porous network.
32061059	11	86	theme	bioactive	1882:1890	arg1	substrate					1892:1900	a highly porous and bioactive substrate	1862:1900	a highly porous and bioactive substrate for dentin tissue engineering	1862:1930	In sum, our findings suggest that CH-Ca scaffolds are attractive candidates for creating a highly porous and bioactive substrate for dentin tissue engineering.
32061059	1	87	theme	dentin	253:258	arg1	regeneration					260:271	dentin regeneration	253:271	dentin regeneration	253:271	The aim of this study was to develop a highly porous calcium-containing chitosan scaffold suitable for dentin regeneration.
32061059	9	88	theme	CH	1513:1514	arg1	scaffold					1516:1523	CH scaffold	1513:1523	CH scaffold	1513:1523	This porous and stable architecture allowed for human dental pulp cells (HDPCs) to spread throughout the scaffold, with cells exhibiting a widely stretched cytoplasm; whereas, the cells seeded onto CH scaffold were organized in clusters.
32061059	11	89	theme	tissue	1913:1918	arg1	engineering					1920:1930	dentin tissue engineering	1906:1930	dentin tissue engineering	1906:1930	In sum, our findings suggest that CH-Ca scaffolds are attractive candidates for creating a highly porous and bioactive substrate for dentin tissue engineering.
32061059	2	90	theme	chemical	364:371	arg1	composition					373:383	chemical composition	364:383	chemical composition	364:383	A calcium hydroxide (Ca[OH]2 ) suspension was used to modulate the degree of porosity and chemical composition of chitosan scaffolds.
33340551	4	0	from	analysis	635:642	arg1	fibroblasts					680:690	fibroblasts	680:690	fibroblasts	680:690	Additional studies included measurement of phosphomannomutase (PMM) activity and analysis of lipid-linked oligosaccharides in fibroblasts.
33340551	6	1	theme	serum	983:987	arg1	isoelectrofocusing					1001:1018	serum transferrin isoelectrofocusing	983:1018	serum transferrin isoelectrofocusing	983:1018	RESULTS Sixty-three individuals were diagnosed covering 14 distinct CDGs; 43 patients diagnosed postnatally revealed a type 1, 14 a type 2, and 2 a normal pattern on serum transferrin isoelectrofocusing.
33340551	5	2	theme	Sanger	693:698	arg1	sequencing					700:709	Sanger sequencing	693:709	Sanger sequencing	693:709	Sanger sequencing and massive parallel sequencing were used to identify causal variants or the affected gene, respectively.
33340551	6	3	theme	a	947:947	arg1	type					949:952	14 a type 2	944:954	14 a type 2	944:954	RESULTS Sixty-three individuals were diagnosed covering 14 distinct CDGs; 43 patients diagnosed postnatally revealed a type 1, 14 a type 2, and 2 a normal pattern on serum transferrin isoelectrofocusing.
33340551	4	4	theme	PMM	617:619	arg1	 activity					621:629	phosphomannomutase (PMM) activity	597:629	phosphomannomutase (PMM) activity	597:629	Additional studies included measurement of phosphomannomutase (PMM) activity and analysis of lipid-linked oligosaccharides in fibroblasts.
33340551	0	5	from	Disorders	11:19	arg1	Decades					54:60	Portugal-Two Decades	41:60	Portugal-Two Decades of	41:63	Congenital Disorders of Glycosylation in Portugal-Two Decades of Experience.
33340551	1	6	theme	new	155:157	arg1	patients					183:190	both new and previously reported patients	150:190	both new and previously reported patients with congenital disorders of glycosylation (CDGs) diagnosed in Portugal over the last 20 years	150:285	OBJECTIVE To describe the clinical, biochemical, and genetic features of both new and previously reported patients with congenital disorders of glycosylation (CDGs) diagnosed in Portugal over the last 20 years.
33340551	6	7	dep	RESULTS	817:823	arg1	diagnosed					854:862	diagnosed	854:862	were diagnosed covering 14 distinct CDGs	849:888	RESULTS Sixty-three individuals were diagnosed covering 14 distinct CDGs; 43 patients diagnosed postnatally revealed a type 1, 14 a type 2, and 2 a normal pattern on serum transferrin isoelectrofocusing.
33340551	4	8	link	lipid-linked	647:658	arg1	oligosaccharides					660:675	lipid-linked oligosaccharides	647:675	lipid-linked oligosaccharides in fibroblasts	647:690	Additional studies included measurement of phosphomannomutase (PMM) activity and analysis of lipid-linked oligosaccharides in fibroblasts.
33340551	10	9	dep	presented	1469:1477	arg1	showing					1508:1514	showing	1508:1514	showing unique clinical phenotypes	1508:1541	CONCLUSIONS Portuguese patients with CDGs are presented in this report, some of them showing unique clinical phenotypes.
33340551	12	10	theme	PMM2-CDG	1700:1707	arg1	spectrum					1688:1695	the mutational spectrum	1673:1695	the mutational spectrum of PMM2-CDG, the most frequent CDG	1673:1730	However, regarding the mutational spectrum of PMM2-CDG, the most frequent CDG, a striking similarity between the 2 populations was found, as only 1 mutated allele found in the Portuguese group has not been reported in Spain.
33340551	2	11	theme	unexplained	338:348	arg1	multisystem					350:360	an unexplained multisystem	335:360	an unexplained multisystem	335:360	STUDY DESIGN The cohort includes patients with an unexplained multisystem or single organ involvement, with or without psychomotor disability.
33340551	6	12	theme	distinct	876:883	arg1	CDGs					885:888	14 distinct CDGs	873:888	14 distinct CDGs	873:888	RESULTS Sixty-three individuals were diagnosed covering 14 distinct CDGs; 43 patients diagnosed postnatally revealed a type 1, 14 a type 2, and 2 a normal pattern on serum transferrin isoelectrofocusing.
33340551	9	13	contain	carry	1291:1295	arg1	the					1277:1279	the	1277:1279	the	1277:1279	Most of the patients (62%) are PMM2-CDG and the remaining carry pathogenic variants in ALG1, ATP6AP1, ATP6AP2, ATP6V0A2, CCDC115, COG1, COG4, DPAGT1, MAN1B1, SLC35A2, SRD5A3, RFT1, or PGM1.
33340551	9	13	contain	carry	1291:1295	arg2	variants					1308:1315	pathogenic variants	1297:1315	pathogenic variants in ALG1, ATP6AP1, ATP6AP2, ATP6V0A2, CCDC115, COG1, COG4, DPAGT1, MAN1B1, SLC35A2, SRD5A3, RFT1, or PGM1	1297:1420	Most of the patients (62%) are PMM2-CDG and the remaining carry pathogenic variants in ALG1, ATP6AP1, ATP6AP2, ATP6V0A2, CCDC115, COG1, COG4, DPAGT1, MAN1B1, SLC35A2, SRD5A3, RFT1, or PGM1.
33340551	4	14	theme	 activity	621:629	arg1	measurement					582:592	measurement	582:592	measurement of phosphomannomutase (PMM) activity	582:629	Additional studies included measurement of phosphomannomutase (PMM) activity and analysis of lipid-linked oligosaccharides in fibroblasts.
33340551	4	14	theme	 activity	621:629	arg1	analysis					635:642	analysis	635:642	analysis of lipid-linked oligosaccharides in fibroblasts	635:690	Additional studies included measurement of phosphomannomutase (PMM) activity and analysis of lipid-linked oligosaccharides in fibroblasts.
33340551	8	15	theme	O-glycosylation	1209:1223	arg1	defect					1225:1230	an associated O-glycosylation defect	1195:1230	an associated O-glycosylation defect	1195:1230	Nine of them presented also a hypoglycosylation pattern on apolipoprotein CIII isoelectrofocusing, pointing to an associated O-glycosylation defect.
33340551	4	16	theme	oligosaccharides	660:675	arg1	measurement					582:592	measurement	582:592	measurement of phosphomannomutase (PMM) activity	582:629	Additional studies included measurement of phosphomannomutase (PMM) activity and analysis of lipid-linked oligosaccharides in fibroblasts.
33340551	4	16	theme	oligosaccharides	660:675	arg1	analysis					635:642	analysis	635:642	analysis of lipid-linked oligosaccharides in fibroblasts	635:690	Additional studies included measurement of phosphomannomutase (PMM) activity and analysis of lipid-linked oligosaccharides in fibroblasts.
33340551	5	17	theme	massive	715:721	arg1	sequencing					732:741	massive parallel sequencing	715:741	massive parallel sequencing	715:741	Sanger sequencing and massive parallel sequencing were used to identify causal variants or the affected gene, respectively.
33340551	8	18	theme	CIII	1158:1161	arg1	isoelectrofocusing					1163:1180	apolipoprotein CIII isoelectrofocusing	1143:1180	apolipoprotein CIII isoelectrofocusing	1143:1180	Nine of them presented also a hypoglycosylation pattern on apolipoprotein CIII isoelectrofocusing, pointing to an associated O-glycosylation defect.
33340551	3	19	theme	apolipoprotein	488:501	arg1	CIII					503:506	apolipoprotein CIII	488:506	apolipoprotein CIII	488:506	Serum sialotransferrin isoforms and, whenever necessary, apolipoprotein CIII isoforms and glycan structures were analyzed.
33340551	5	20	theme	affected	788:795	arg1	gene					797:800	the affected gene	784:800	the affected gene	784:800	Sanger sequencing and massive parallel sequencing were used to identify causal variants or the affected gene, respectively.
33340551	1	21	theme	biochemical	113:123	arg1	features					138:145	the clinical, biochemical, and genetic features	99:145	the clinical, biochemical, and genetic features of both new and previously reported patients with congenital disorders of glycosylation (CDGs) diagnosed in Portugal over the last 20 years	99:285	OBJECTIVE To describe the clinical, biochemical, and genetic features of both new and previously reported patients with congenital disorders of glycosylation (CDGs) diagnosed in Portugal over the last 20 years.
33340551	8	22	theme	them	1092:1095	arg1	them					1092:1095	them	1092:1095	them	1092:1095	Nine of them presented also a hypoglycosylation pattern on apolipoprotein CIII isoelectrofocusing, pointing to an associated O-glycosylation defect.
33340551	8	22	theme	them	1092:1095	arg1	Nine					1084:1087	Nine	1084:1087	Nine	1084:1087	Nine of them presented also a hypoglycosylation pattern on apolipoprotein CIII isoelectrofocusing, pointing to an associated O-glycosylation defect.
33340551	2	23	theme	psychomotor	407:417	arg1	disability					419:428	psychomotor disability	407:428	psychomotor disability	407:428	STUDY DESIGN The cohort includes patients with an unexplained multisystem or single organ involvement, with or without psychomotor disability.
33340551	10	24	theme	unique	1516:1521	arg1	phenotypes					1532:1541	unique clinical phenotypes	1516:1541	unique clinical phenotypes	1516:1541	CONCLUSIONS Portuguese patients with CDGs are presented in this report, some of them showing unique clinical phenotypes.
33340551	10	25	with	patients	1446:1453	arg1	CDGs					1460:1463	CDGs	1460:1463	CDGs	1460:1463	CONCLUSIONS Portuguese patients with CDGs are presented in this report, some of them showing unique clinical phenotypes.
33340551	3	26	theme	sialotransferrin	437:452	arg1	isoforms					454:461	Serum sialotransferrin isoforms	431:461	Serum sialotransferrin isoforms	431:461	Serum sialotransferrin isoforms and, whenever necessary, apolipoprotein CIII isoforms and glycan structures were analyzed.
33340551	3	27	theme	Serum	431:435	arg1	isoforms					454:461	Serum sialotransferrin isoforms	431:461	Serum sialotransferrin isoforms	431:461	Serum sialotransferrin isoforms and, whenever necessary, apolipoprotein CIII isoforms and glycan structures were analyzed.
33340551	3	28	dep	isoforms	508:515	arg1	CIII					503:506	apolipoprotein CIII	488:506	apolipoprotein CIII	488:506	Serum sialotransferrin isoforms and, whenever necessary, apolipoprotein CIII isoforms and glycan structures were analyzed.
33340551	5	29	used	used	748:751	arg2	sequencing					700:709	Sanger sequencing	693:709	Sanger sequencing	693:709	Sanger sequencing and massive parallel sequencing were used to identify causal variants or the affected gene, respectively.
33340551	5	29	used	used	748:751	arg2	sequencing					732:741	massive parallel sequencing	715:741	massive parallel sequencing	715:741	Sanger sequencing and massive parallel sequencing were used to identify causal variants or the affected gene, respectively.
33340551	11	30	theme	reported	1629:1636	arg1	cohort					1646:1651	a previously reported Spanish cohort	1616:1651	a previously reported Spanish cohort	1616:1651	Among the 14 genes mutated in Portuguese individuals, 8 are shared with a previously reported Spanish cohort.
33340551	10	31	theme	clinical	1523:1530	arg1	phenotypes					1532:1541	unique clinical phenotypes	1516:1541	unique clinical phenotypes	1516:1541	CONCLUSIONS Portuguese patients with CDGs are presented in this report, some of them showing unique clinical phenotypes.
33340551	8	32	theme	apolipoprotein	1143:1156	arg1	CIII					1158:1161	apolipoprotein CIII	1143:1161	apolipoprotein CIII isoelectrofocusing	1143:1180	Nine of them presented also a hypoglycosylation pattern on apolipoprotein CIII isoelectrofocusing, pointing to an associated O-glycosylation defect.
33340551	1	33	theme	reported	174:181	arg1	patients					183:190	both new and previously reported patients	150:190	both new and previously reported patients with congenital disorders of glycosylation (CDGs) diagnosed in Portugal over the last 20 years	150:285	OBJECTIVE To describe the clinical, biochemical, and genetic features of both new and previously reported patients with congenital disorders of glycosylation (CDGs) diagnosed in Portugal over the last 20 years.
33340551	0	34	theme	Congenital	0:9	arg1	Disorders					11:19	Congenital Disorders	0:19	Congenital Disorders of Glycosylation in Portugal-Two Decades of	0:63	Congenital Disorders of Glycosylation in Portugal-Two Decades of Experience.
33340551	10	35	theme	Portuguese	1435:1444	arg1	patients					1446:1453	CONCLUSIONS Portuguese patients	1423:1453	CONCLUSIONS Portuguese patients with CDGs	1423:1463	CONCLUSIONS Portuguese patients with CDGs are presented in this report, some of them showing unique clinical phenotypes.
33340551	11	36	theme	Spanish	1638:1644	arg1	cohort					1646:1651	a previously reported Spanish cohort	1616:1651	a previously reported Spanish cohort	1616:1651	Among the 14 genes mutated in Portuguese individuals, 8 are shared with a previously reported Spanish cohort.
33340551	8	37	theme	associated	1198:1207	arg1	defect					1225:1230	an associated O-glycosylation defect	1195:1230	an associated O-glycosylation defect	1195:1230	Nine of them presented also a hypoglycosylation pattern on apolipoprotein CIII isoelectrofocusing, pointing to an associated O-glycosylation defect.
33340551	2	38	theme	organ	372:376	arg1	involvement					378:388	single organ involvement	365:388	single organ involvement	365:388	STUDY DESIGN The cohort includes patients with an unexplained multisystem or single organ involvement, with or without psychomotor disability.
33340551	0	39	theme	Glycosylation	24:36	arg1	Disorders					11:19	Congenital Disorders	0:19	Congenital Disorders of Glycosylation in Portugal-Two Decades of	0:63	Congenital Disorders of Glycosylation in Portugal-Two Decades of Experience.
33340551	1	40	theme	last	273:276	arg1	20 years					278:285	the last 20 years	269:285	the last 20 years	269:285	OBJECTIVE To describe the clinical, biochemical, and genetic features of both new and previously reported patients with congenital disorders of glycosylation (CDGs) diagnosed in Portugal over the last 20 years.
33340551	8	41	theme	hypoglycosylation	1114:1130	arg1	pattern					1132:1138	a hypoglycosylation pattern	1112:1138	a hypoglycosylation pattern on apolipoprotein CIII isoelectrofocusing	1112:1180	Nine of them presented also a hypoglycosylation pattern on apolipoprotein CIII isoelectrofocusing, pointing to an associated O-glycosylation defect.
33340551	6	42	theme	Sixty-three	825:835	arg1	individuals					837:847	Sixty-three individuals	825:847	Sixty-three individuals	825:847	RESULTS Sixty-three individuals were diagnosed covering 14 distinct CDGs; 43 patients diagnosed postnatally revealed a type 1, 14 a type 2, and 2 a normal pattern on serum transferrin isoelectrofocusing.
33340551	1	43	theme	clinical	103:110	arg1	features					138:145	the clinical, biochemical, and genetic features	99:145	the clinical, biochemical, and genetic features of both new and previously reported patients with congenital disorders of glycosylation (CDGs) diagnosed in Portugal over the last 20 years	99:285	OBJECTIVE To describe the clinical, biochemical, and genetic features of both new and previously reported patients with congenital disorders of glycosylation (CDGs) diagnosed in Portugal over the last 20 years.
33340551	4	44	from	fibroblasts	680:690	arg1	measurement					582:592	measurement	582:592	measurement of phosphomannomutase (PMM) activity	582:629	Additional studies included measurement of phosphomannomutase (PMM) activity and analysis of lipid-linked oligosaccharides in fibroblasts.
33340551	4	44	from	fibroblasts	680:690	arg1	analysis					635:642	analysis	635:642	analysis of lipid-linked oligosaccharides in fibroblasts	635:690	Additional studies included measurement of phosphomannomutase (PMM) activity and analysis of lipid-linked oligosaccharides in fibroblasts.
33340551	4	45	theme	phosphomannomutase	597:614	arg1	 activity					621:629	phosphomannomutase (PMM) activity	597:629	phosphomannomutase (PMM) activity	597:629	Additional studies included measurement of phosphomannomutase (PMM) activity and analysis of lipid-linked oligosaccharides in fibroblasts.
33340551	12	46	theme	mutated	1802:1808	arg1	allele					1810:1815	only 1 mutated allele	1795:1815	only 1 mutated allele found in the Portuguese group	1795:1845	However, regarding the mutational spectrum of PMM2-CDG, the most frequent CDG, a striking similarity between the 2 populations was found, as only 1 mutated allele found in the Portuguese group has not been reported in Spain.
33340551	2	47	with	patients	321:328	arg1	multisystem					350:360	an unexplained multisystem	335:360	an unexplained multisystem	335:360	STUDY DESIGN The cohort includes patients with an unexplained multisystem or single organ involvement, with or without psychomotor disability.
33340551	2	47	with	patients	321:328	arg1	involvement					378:388	single organ involvement	365:388	single organ involvement	365:388	STUDY DESIGN The cohort includes patients with an unexplained multisystem or single organ involvement, with or without psychomotor disability.
33340551	2	48	theme	STUDY	288:292	arg1	DESIGN					294:299	STUDY DESIGN The cohort	288:310	STUDY DESIGN The cohort	288:310	STUDY DESIGN The cohort includes patients with an unexplained multisystem or single organ involvement, with or without psychomotor disability.
33340551	1	49	theme	congenital	197:206	arg1	CDGs					236:239	CDGs	236:239	CDGs	236:239	OBJECTIVE To describe the clinical, biochemical, and genetic features of both new and previously reported patients with congenital disorders of glycosylation (CDGs) diagnosed in Portugal over the last 20 years.
33340551	1	49	theme	congenital	197:206	arg1	disorders					208:216	congenital disorders	197:216	congenital disorders of glycosylation (CDGs) diagnosed in Portugal over the last 20 years	197:285	OBJECTIVE To describe the clinical, biochemical, and genetic features of both new and previously reported patients with congenital disorders of glycosylation (CDGs) diagnosed in Portugal over the last 20 years.
33340551	0	50	theme	Portugal-Two	41:52	arg1	Decades					54:60	Portugal-Two Decades	41:60	Portugal-Two Decades of	41:63	Congenital Disorders of Glycosylation in Portugal-Two Decades of Experience.
33340551	5	51	theme	causal	765:770	arg1	variants					772:779	causal variants	765:779	causal variants	765:779	Sanger sequencing and massive parallel sequencing were used to identify causal variants or the affected gene, respectively.
33340551	1	52	theme	patients	183:190	arg1	features					138:145	the clinical, biochemical, and genetic features	99:145	the clinical, biochemical, and genetic features of both new and previously reported patients with congenital disorders of glycosylation (CDGs) diagnosed in Portugal over the last 20 years	99:285	OBJECTIVE To describe the clinical, biochemical, and genetic features of both new and previously reported patients with congenital disorders of glycosylation (CDGs) diagnosed in Portugal over the last 20 years.
33340551	12	53	theme	Portuguese	1830:1839	arg1	group					1841:1845	the Portuguese group	1826:1845	the Portuguese group	1826:1845	However, regarding the mutational spectrum of PMM2-CDG, the most frequent CDG, a striking similarity between the 2 populations was found, as only 1 mutated allele found in the Portuguese group has not been reported in Spain.
33340551	6	54	from	type	936:939	arg1	isoelectrofocusing					1001:1018	serum transferrin isoelectrofocusing	983:1018	serum transferrin isoelectrofocusing	983:1018	RESULTS Sixty-three individuals were diagnosed covering 14 distinct CDGs; 43 patients diagnosed postnatally revealed a type 1, 14 a type 2, and 2 a normal pattern on serum transferrin isoelectrofocusing.
33340551	4	55	from	measurement	582:592	arg1	fibroblasts					680:690	fibroblasts	680:690	fibroblasts	680:690	Additional studies included measurement of phosphomannomutase (PMM) activity and analysis of lipid-linked oligosaccharides in fibroblasts.
33340551	6	56	theme	normal	965:970	arg1	pattern					972:978	a normal pattern	963:978	a normal pattern	963:978	RESULTS Sixty-three individuals were diagnosed covering 14 distinct CDGs; 43 patients diagnosed postnatally revealed a type 1, 14 a type 2, and 2 a normal pattern on serum transferrin isoelectrofocusing.
33340551	5	57	theme	parallel	723:730	arg1	sequencing					732:741	massive parallel sequencing	715:741	massive parallel sequencing	715:741	Sanger sequencing and massive parallel sequencing were used to identify causal variants or the affected gene, respectively.
33340551	6	58	theme	transferrin	989:999	arg1	isoelectrofocusing					1001:1018	serum transferrin isoelectrofocusing	983:1018	serum transferrin isoelectrofocusing	983:1018	RESULTS Sixty-three individuals were diagnosed covering 14 distinct CDGs; 43 patients diagnosed postnatally revealed a type 1, 14 a type 2, and 2 a normal pattern on serum transferrin isoelectrofocusing.
33340551	7	59	theme	whole	1060:1064	arg1	sequencing					1072:1081	whole exome sequencing	1060:1081	whole exome sequencing	1060:1081	The latter patients were identified by whole exome sequencing.
33340551	2	60	dep	DESIGN	294:299	arg1	cohort					305:310	The cohort	301:310	STUDY DESIGN The cohort	288:310	STUDY DESIGN The cohort includes patients with an unexplained multisystem or single organ involvement, with or without psychomotor disability.
33340551	6	61	dep	type	936:939	arg1	pattern					972:978	a normal pattern	963:978	a normal pattern	963:978	RESULTS Sixty-three individuals were diagnosed covering 14 distinct CDGs; 43 patients diagnosed postnatally revealed a type 1, 14 a type 2, and 2 a normal pattern on serum transferrin isoelectrofocusing.
33340551	6	61	dep	type	936:939	arg1	a					934:934	a	934:934	a	934:934	RESULTS Sixty-three individuals were diagnosed covering 14 distinct CDGs; 43 patients diagnosed postnatally revealed a type 1, 14 a type 2, and 2 a normal pattern on serum transferrin isoelectrofocusing.
33340551	1	62	with	patients	183:190	arg1	CDGs					236:239	CDGs	236:239	CDGs	236:239	OBJECTIVE To describe the clinical, biochemical, and genetic features of both new and previously reported patients with congenital disorders of glycosylation (CDGs) diagnosed in Portugal over the last 20 years.
33340551	1	62	with	patients	183:190	arg1	disorders					208:216	congenital disorders	197:216	congenital disorders of glycosylation (CDGs) diagnosed in Portugal over the last 20 years	197:285	OBJECTIVE To describe the clinical, biochemical, and genetic features of both new and previously reported patients with congenital disorders of glycosylation (CDGs) diagnosed in Portugal over the last 20 years.
33340551	3	63	theme	glycan	521:526	arg1	structures					528:537	glycan structures	521:537	glycan structures	521:537	Serum sialotransferrin isoforms and, whenever necessary, apolipoprotein CIII isoforms and glycan structures were analyzed.
33340551	12	64	theme	mutational	1677:1686	arg1	spectrum					1688:1695	the mutational spectrum	1673:1695	the mutational spectrum of PMM2-CDG, the most frequent CDG	1673:1730	However, regarding the mutational spectrum of PMM2-CDG, the most frequent CDG, a striking similarity between the 2 populations was found, as only 1 mutated allele found in the Portuguese group has not been reported in Spain.
33340551	4	65	theme	Additional	554:563	arg1	studies					565:571	Additional studies	554:571	Additional studies	554:571	Additional studies included measurement of phosphomannomutase (PMM) activity and analysis of lipid-linked oligosaccharides in fibroblasts.
33340551	7	66	theme	exome	1066:1070	arg1	sequencing					1072:1081	whole exome sequencing	1060:1081	whole exome sequencing	1060:1081	The latter patients were identified by whole exome sequencing.
33340551	9	67	theme	pathogenic	1297:1306	arg1	variants					1308:1315	pathogenic variants	1297:1315	pathogenic variants in ALG1, ATP6AP1, ATP6AP2, ATP6V0A2, CCDC115, COG1, COG4, DPAGT1, MAN1B1, SLC35A2, SRD5A3, RFT1, or PGM1	1297:1420	Most of the patients (62%) are PMM2-CDG and the remaining carry pathogenic variants in ALG1, ATP6AP1, ATP6AP2, ATP6V0A2, CCDC115, COG1, COG4, DPAGT1, MAN1B1, SLC35A2, SRD5A3, RFT1, or PGM1.
33340551	12	68	theme	frequent	1719:1726	arg1	CDG					1728:1730	the most frequent CDG	1710:1730	the most frequent CDG	1710:1730	However, regarding the mutational spectrum of PMM2-CDG, the most frequent CDG, a striking similarity between the 2 populations was found, as only 1 mutated allele found in the Portuguese group has not been reported in Spain.
33340551	12	68	theme	frequent	1719:1726	arg1	PMM2-CDG					1700:1707	PMM2-CDG	1700:1707	PMM2-CDG	1700:1707	However, regarding the mutational spectrum of PMM2-CDG, the most frequent CDG, a striking similarity between the 2 populations was found, as only 1 mutated allele found in the Portuguese group has not been reported in Spain.
33340551	12	69	located	found	1817:1821	arg1	group					1841:1845	the Portuguese group	1826:1845	the Portuguese group	1826:1845	However, regarding the mutational spectrum of PMM2-CDG, the most frequent CDG, a striking similarity between the 2 populations was found, as only 1 mutated allele found in the Portuguese group has not been reported in Spain.
33340551	12	69	located	found	1817:1821	arg2	allele					1810:1815	only 1 mutated allele	1795:1815	only 1 mutated allele found in the Portuguese group	1795:1845	However, regarding the mutational spectrum of PMM2-CDG, the most frequent CDG, a striking similarity between the 2 populations was found, as only 1 mutated allele found in the Portuguese group has not been reported in Spain.
33340551	1	70	theme	glycosylation	221:233	arg1	CDGs					236:239	CDGs	236:239	CDGs	236:239	OBJECTIVE To describe the clinical, biochemical, and genetic features of both new and previously reported patients with congenital disorders of glycosylation (CDGs) diagnosed in Portugal over the last 20 years.
33340551	1	70	theme	glycosylation	221:233	arg1	disorders					208:216	congenital disorders	197:216	congenital disorders of glycosylation (CDGs) diagnosed in Portugal over the last 20 years	197:285	OBJECTIVE To describe the clinical, biochemical, and genetic features of both new and previously reported patients with congenital disorders of glycosylation (CDGs) diagnosed in Portugal over the last 20 years.
33340551	7	71	theme	latter	1025:1030	arg1	patients					1032:1039	The latter patients	1021:1039	The latter patients	1021:1039	The latter patients were identified by whole exome sequencing.
33340551	1	72	theme	genetic	130:136	arg1	features					138:145	the clinical, biochemical, and genetic features	99:145	the clinical, biochemical, and genetic features of both new and previously reported patients with congenital disorders of glycosylation (CDGs) diagnosed in Portugal over the last 20 years	99:285	OBJECTIVE To describe the clinical, biochemical, and genetic features of both new and previously reported patients with congenital disorders of glycosylation (CDGs) diagnosed in Portugal over the last 20 years.
33340551	3	73	theme	necessary	477:485	arg1	isoforms					508:515	, whenever necessary, apolipoprotein CIII isoforms	466:515	isoforms	508:515	Serum sialotransferrin isoforms and, whenever necessary, apolipoprotein CIII isoforms and glycan structures were analyzed.
33340551	8	74	from	pattern	1132:1138	arg1	isoelectrofocusing					1163:1180	apolipoprotein CIII isoelectrofocusing	1143:1180	apolipoprotein CIII isoelectrofocusing	1143:1180	Nine of them presented also a hypoglycosylation pattern on apolipoprotein CIII isoelectrofocusing, pointing to an associated O-glycosylation defect.
33340551	9	75	from	variants	1308:1315	arg1	SLC35A2					1391:1397	SLC35A2	1391:1397	SLC35A2	1391:1397	Most of the patients (62%) are PMM2-CDG and the remaining carry pathogenic variants in ALG1, ATP6AP1, ATP6AP2, ATP6V0A2, CCDC115, COG1, COG4, DPAGT1, MAN1B1, SLC35A2, SRD5A3, RFT1, or PGM1.
33340551	9	75	from	variants	1308:1315	arg1	ATP6V0A2					1344:1351	ATP6V0A2	1344:1351	ATP6V0A2	1344:1351	Most of the patients (62%) are PMM2-CDG and the remaining carry pathogenic variants in ALG1, ATP6AP1, ATP6AP2, ATP6V0A2, CCDC115, COG1, COG4, DPAGT1, MAN1B1, SLC35A2, SRD5A3, RFT1, or PGM1.
33340551	9	75	from	variants	1308:1315	arg1	PGM1					1417:1420	PGM1	1417:1420	PGM1	1417:1420	Most of the patients (62%) are PMM2-CDG and the remaining carry pathogenic variants in ALG1, ATP6AP1, ATP6AP2, ATP6V0A2, CCDC115, COG1, COG4, DPAGT1, MAN1B1, SLC35A2, SRD5A3, RFT1, or PGM1.
33340551	9	75	from	variants	1308:1315	arg1	COG4					1369:1372	COG4	1369:1372	COG4	1369:1372	Most of the patients (62%) are PMM2-CDG and the remaining carry pathogenic variants in ALG1, ATP6AP1, ATP6AP2, ATP6V0A2, CCDC115, COG1, COG4, DPAGT1, MAN1B1, SLC35A2, SRD5A3, RFT1, or PGM1.
33340551	9	75	from	variants	1308:1315	arg1	ALG1					1320:1323	ALG1	1320:1323	ALG1	1320:1323	Most of the patients (62%) are PMM2-CDG and the remaining carry pathogenic variants in ALG1, ATP6AP1, ATP6AP2, ATP6V0A2, CCDC115, COG1, COG4, DPAGT1, MAN1B1, SLC35A2, SRD5A3, RFT1, or PGM1.
33340551	9	75	from	variants	1308:1315	arg1	DPAGT1					1375:1380	DPAGT1	1375:1380	DPAGT1	1375:1380	Most of the patients (62%) are PMM2-CDG and the remaining carry pathogenic variants in ALG1, ATP6AP1, ATP6AP2, ATP6V0A2, CCDC115, COG1, COG4, DPAGT1, MAN1B1, SLC35A2, SRD5A3, RFT1, or PGM1.
33340551	9	75	from	variants	1308:1315	arg1	SRD5A3					1400:1405	SRD5A3	1400:1405	SRD5A3	1400:1405	Most of the patients (62%) are PMM2-CDG and the remaining carry pathogenic variants in ALG1, ATP6AP1, ATP6AP2, ATP6V0A2, CCDC115, COG1, COG4, DPAGT1, MAN1B1, SLC35A2, SRD5A3, RFT1, or PGM1.
33340551	9	75	from	variants	1308:1315	arg1	ATP6AP1					1326:1332	ATP6AP1	1326:1332	ATP6AP1	1326:1332	Most of the patients (62%) are PMM2-CDG and the remaining carry pathogenic variants in ALG1, ATP6AP1, ATP6AP2, ATP6V0A2, CCDC115, COG1, COG4, DPAGT1, MAN1B1, SLC35A2, SRD5A3, RFT1, or PGM1.
33340551	9	75	from	variants	1308:1315	arg1	CCDC115					1354:1360	CCDC115	1354:1360	CCDC115	1354:1360	Most of the patients (62%) are PMM2-CDG and the remaining carry pathogenic variants in ALG1, ATP6AP1, ATP6AP2, ATP6V0A2, CCDC115, COG1, COG4, DPAGT1, MAN1B1, SLC35A2, SRD5A3, RFT1, or PGM1.
33340551	9	75	from	variants	1308:1315	arg1	RFT1					1408:1411	RFT1	1408:1411	RFT1	1408:1411	Most of the patients (62%) are PMM2-CDG and the remaining carry pathogenic variants in ALG1, ATP6AP1, ATP6AP2, ATP6V0A2, CCDC115, COG1, COG4, DPAGT1, MAN1B1, SLC35A2, SRD5A3, RFT1, or PGM1.
33340551	9	75	from	variants	1308:1315	arg1	COG1					1363:1366	COG1	1363:1366	COG1	1363:1366	Most of the patients (62%) are PMM2-CDG and the remaining carry pathogenic variants in ALG1, ATP6AP1, ATP6AP2, ATP6V0A2, CCDC115, COG1, COG4, DPAGT1, MAN1B1, SLC35A2, SRD5A3, RFT1, or PGM1.
33340551	9	75	from	variants	1308:1315	arg1	MAN1B1					1383:1388	MAN1B1	1383:1388	MAN1B1	1383:1388	Most of the patients (62%) are PMM2-CDG and the remaining carry pathogenic variants in ALG1, ATP6AP1, ATP6AP2, ATP6V0A2, CCDC115, COG1, COG4, DPAGT1, MAN1B1, SLC35A2, SRD5A3, RFT1, or PGM1.
33340551	9	75	from	variants	1308:1315	arg1	ATP6AP2					1335:1341	ATP6AP2	1335:1341	ATP6AP2	1335:1341	Most of the patients (62%) are PMM2-CDG and the remaining carry pathogenic variants in ALG1, ATP6AP1, ATP6AP2, ATP6V0A2, CCDC115, COG1, COG4, DPAGT1, MAN1B1, SLC35A2, SRD5A3, RFT1, or PGM1.
33340551	12	76	theme	striking	1735:1742	arg1	similarity					1744:1753	a striking similarity	1733:1753	a striking similarity between the 2 populations	1733:1779	However, regarding the mutational spectrum of PMM2-CDG, the most frequent CDG, a striking similarity between the 2 populations was found, as only 1 mutated allele found in the Portuguese group has not been reported in Spain.
33340551	2	77	theme	single	365:370	arg1	involvement					378:388	single organ involvement	365:388	single organ involvement	365:388	STUDY DESIGN The cohort includes patients with an unexplained multisystem or single organ involvement, with or without psychomotor disability.
33340551	4	78	theme	lipid-linked	647:658	arg1	oligosaccharides					660:675	lipid-linked oligosaccharides	647:675	lipid-linked oligosaccharides in fibroblasts	647:690	Additional studies included measurement of phosphomannomutase (PMM) activity and analysis of lipid-linked oligosaccharides in fibroblasts.
33340551	6	79	from	type	949:952	arg1	isoelectrofocusing					1001:1018	serum transferrin isoelectrofocusing	983:1018	serum transferrin isoelectrofocusing	983:1018	RESULTS Sixty-three individuals were diagnosed covering 14 distinct CDGs; 43 patients diagnosed postnatally revealed a type 1, 14 a type 2, and 2 a normal pattern on serum transferrin isoelectrofocusing.
33340551	4	80	from	oligosaccharides	660:675	arg1	fibroblasts					680:690	fibroblasts	680:690	fibroblasts	680:690	Additional studies included measurement of phosphomannomutase (PMM) activity and analysis of lipid-linked oligosaccharides in fibroblasts.
33340551	11	81	theme	Portuguese	1574:1583	arg1	individuals					1585:1595	Portuguese individuals	1574:1595	Portuguese individuals	1574:1595	Among the 14 genes mutated in Portuguese individuals, 8 are shared with a previously reported Spanish cohort.
33340551	10	82	theme	CONCLUSIONS	1423:1433	arg1	patients					1446:1453	CONCLUSIONS Portuguese patients	1423:1453	CONCLUSIONS Portuguese patients with CDGs	1423:1463	CONCLUSIONS Portuguese patients with CDGs are presented in this report, some of them showing unique clinical phenotypes.
34440734	3	0	theme	NP	296:297	arg1	effect					319:324	their effect	313:324	their effect on different developmental processes	313:361	Knowledge about the uptake of NP by plants and their effect on different developmental processes is still insufficient.
34440734	3	0	theme	NP	296:297	arg1	uptake					286:291	the uptake	282:291	the uptake of NP by plants	282:307	Knowledge about the uptake of NP by plants and their effect on different developmental processes is still insufficient.
34440734	0	1	from	Modifications	27:39	arg1	Composition					54:64	Cell Wall Composition	44:64	Cell Wall Composition	44:64	Gold Nanoparticles-Induced Modifications in Cell Wall Composition in Barley Roots.
34440734	0	1	from	Modifications	27:39	arg1	Roots					76:80	Barley Roots	69:80	Barley Roots	69:80	Gold Nanoparticles-Induced Modifications in Cell Wall Composition in Barley Roots.
34440734	6	2	theme	root	847:850	arg1	cells					852:856	root cells	847:856	root cells	847:856	The obtained results indicate that AuNP with different surface charges affects the presence and distribution of selected pectic and arabinogalactan protein (AGP) epitopes in the walls of root cells.
34440734	4	3	theme	gold	540:543	arg1	nanoparticles					545:557	gold nanoparticles	540:557	gold nanoparticles	540:557	Our studies concerned analyses of the changes in the chemical components of the cell walls of Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP).
34440734	4	4	theme	walls	471:475	arg1	components					448:457	the chemical components	435:457	the chemical components of the cell walls of Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP)	435:564	Our studies concerned analyses of the changes in the chemical components of the cell walls of Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP).
34440734	4	5	theme	vulgare	488:494	arg1	roots					499:503	Hordeum vulgare L. roots	480:503	Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP)	480:564	Our studies concerned analyses of the changes in the chemical components of the cell walls of Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP).
34440734	5	6	theme	immunohistological	605:622	arg1	method					624:629	the immunohistological method	601:629	the immunohistological method	601:629	The analyses were performed using the immunohistological method and fluorescence microscopy.
34440734	6	7	theme	protein	808:814	arg1	epitopes					822:829	selected pectic and arabinogalactan protein (AGP) epitopes	772:829	selected pectic and arabinogalactan protein (AGP) epitopes	772:829	The obtained results indicate that AuNP with different surface charges affects the presence and distribution of selected pectic and arabinogalactan protein (AGP) epitopes in the walls of root cells.
34440734	6	8	dep	presence	743:750	arg1	the					739:741	the	739:741	the	739:741	The obtained results indicate that AuNP with different surface charges affects the presence and distribution of selected pectic and arabinogalactan protein (AGP) epitopes in the walls of root cells.
34440734	4	9	theme	Hordeum	480:486	arg1	vulgare					488:494	Hordeum vulgare	480:494	Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP)	480:564	Our studies concerned analyses of the changes in the chemical components of the cell walls of Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP).
34440734	6	10	theme	arabinogalactan	792:806	arg1	AGP					817:819	AGP	817:819	AGP	817:819	The obtained results indicate that AuNP with different surface charges affects the presence and distribution of selected pectic and arabinogalactan protein (AGP) epitopes in the walls of root cells.
34440734	6	10	theme	arabinogalactan	792:806	arg1	protein					808:814	pectic and arabinogalactan protein	781:814	protein	808:814	The obtained results indicate that AuNP with different surface charges affects the presence and distribution of selected pectic and arabinogalactan protein (AGP) epitopes in the walls of root cells.
34440734	3	11	theme	different	329:337	arg1	processes					353:361	different developmental processes	329:361	different developmental processes	329:361	Knowledge about the uptake of NP by plants and their effect on different developmental processes is still insufficient.
34440734	4	12	theme	nanoparticles	545:557	arg1	presence					528:535	the presence	524:535	the presence of gold nanoparticles	524:557	Our studies concerned analyses of the changes in the chemical components of the cell walls of Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP).
34440734	6	13	theme	pectic	781:786	arg1	AGP					817:819	AGP	817:819	AGP	817:819	The obtained results indicate that AuNP with different surface charges affects the presence and distribution of selected pectic and arabinogalactan protein (AGP) epitopes in the walls of root cells.
34440734	6	13	theme	pectic	781:786	arg1	protein					808:814	pectic and arabinogalactan protein	781:814	protein	808:814	The obtained results indicate that AuNP with different surface charges affects the presence and distribution of selected pectic and arabinogalactan protein (AGP) epitopes in the walls of root cells.
34440734	4	14	theme	cell	466:469	arg1	walls					471:475	the cell walls	462:475	the cell walls of Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP)	462:564	Our studies concerned analyses of the changes in the chemical components of the cell walls of Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP).
34440734	6	15	theme	selected	772:779	arg1	epitopes					822:829	selected pectic and arabinogalactan protein (AGP) epitopes	772:829	selected pectic and arabinogalactan protein (AGP) epitopes	772:829	The obtained results indicate that AuNP with different surface charges affects the presence and distribution of selected pectic and arabinogalactan protein (AGP) epitopes in the walls of root cells.
34440734	0	16	theme	Nanoparticles-Induced	5:25	arg1	Modifications					27:39	Gold Nanoparticles-Induced Modifications	0:39	Gold Nanoparticles-Induced Modifications in Cell Wall Composition in Barley Roots	0:80	Gold Nanoparticles-Induced Modifications in Cell Wall Composition in Barley Roots.
34440734	4	17	dep	grown	515:519	arg1	AuNP					560:563	AuNP	560:563	AuNP	560:563	Our studies concerned analyses of the changes in the chemical components of the cell walls of Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP).
34440734	1	18	theme	increased	87:95	arg1	use					97:99	The increased use	83:99	The increased use of nanoparticles (NP) in different industries	83:145	The increased use of nanoparticles (NP) in different industries inevitably results in their release into the environment.
34440734	6	19	theme	obtained	664:671	arg1	results					673:679	The obtained results	660:679	The obtained results	660:679	The obtained results indicate that AuNP with different surface charges affects the presence and distribution of selected pectic and arabinogalactan protein (AGP) epitopes in the walls of root cells.
34440734	0	20	theme	Gold	0:3	arg1	Modifications					27:39	Gold Nanoparticles-Induced Modifications	0:39	Gold Nanoparticles-Induced Modifications in Cell Wall Composition in Barley Roots	0:80	Gold Nanoparticles-Induced Modifications in Cell Wall Composition in Barley Roots.
34440734	4	21	from	components	448:457	arg1	analyses					408:415	analyses	408:415	analyses of the changes in the chemical components of the cell walls of Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP)	408:564	Our studies concerned analyses of the changes in the chemical components of the cell walls of Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP).
34440734	6	22	attach	presence	743:750	arg1	walls					838:842	the walls	834:842	the walls of root cells	834:856	The obtained results indicate that AuNP with different surface charges affects the presence and distribution of selected pectic and arabinogalactan protein (AGP) epitopes in the walls of root cells.
34440734	6	22	attach	presence	743:750	arg2	epitopes					822:829	selected pectic and arabinogalactan protein (AGP) epitopes	772:829	selected pectic and arabinogalactan protein (AGP) epitopes	772:829	The obtained results indicate that AuNP with different surface charges affects the presence and distribution of selected pectic and arabinogalactan protein (AGP) epitopes in the walls of root cells.
34440734	4	23	theme	changes	424:430	arg1	analyses					408:415	analyses	408:415	analyses of the changes in the chemical components of the cell walls of Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP)	408:564	Our studies concerned analyses of the changes in the chemical components of the cell walls of Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP).
34440734	3	24	theme	developmental	339:351	arg1	processes					353:361	different developmental processes	329:361	different developmental processes	329:361	Knowledge about the uptake of NP by plants and their effect on different developmental processes is still insufficient.
34440734	6	25	from	distribution	756:767	arg1	walls					838:842	the walls	834:842	the walls of root cells	834:856	The obtained results indicate that AuNP with different surface charges affects the presence and distribution of selected pectic and arabinogalactan protein (AGP) epitopes in the walls of root cells.
34440734	1	26	theme	nanoparticles	104:116	arg1	use					97:99	The increased use	83:99	The increased use of nanoparticles (NP) in different industries	83:145	The increased use of nanoparticles (NP) in different industries inevitably results in their release into the environment.
34440734	0	27	theme	Wall	49:52	arg1	Composition					54:64	Cell Wall Composition	44:64	Cell Wall Composition	44:64	Gold Nanoparticles-Induced Modifications in Cell Wall Composition in Barley Roots.
34440734	3	28	from	effect	319:324	arg1	processes					353:361	different developmental processes	329:361	different developmental processes	329:361	Knowledge about the uptake of NP by plants and their effect on different developmental processes is still insufficient.
34440734	0	29	theme	Cell	44:47	arg1	Composition					54:64	Cell Wall Composition	44:64	Cell Wall Composition	44:64	Gold Nanoparticles-Induced Modifications in Cell Wall Composition in Barley Roots.
34440734	4	30	theme	roots	499:503	arg1	walls					471:475	the cell walls	462:475	the cell walls of Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP)	462:564	Our studies concerned analyses of the changes in the chemical components of the cell walls of Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP).
34440734	4	31	from	changes	424:430	arg1	components					448:457	the chemical components	435:457	the chemical components of the cell walls of Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP)	435:564	Our studies concerned analyses of the changes in the chemical components of the cell walls of Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP).
34440734	4	32	theme	chemical	439:446	arg1	components					448:457	the chemical components	435:457	the chemical components of the cell walls of Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP)	435:564	Our studies concerned analyses of the changes in the chemical components of the cell walls of Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP).
34440734	4	33	theme	L.	496:497	arg1	roots					499:503	Hordeum vulgare L. roots	480:503	Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP)	480:564	Our studies concerned analyses of the changes in the chemical components of the cell walls of Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP).
34440734	5	34	theme	fluorescence	635:646	arg1	microscopy					648:657	fluorescence microscopy	635:657	fluorescence microscopy	635:657	The analyses were performed using the immunohistological method and fluorescence microscopy.
34440734	6	35	theme	cells	852:856	arg1	walls					838:842	the walls	834:842	the walls of root cells	834:856	The obtained results indicate that AuNP with different surface charges affects the presence and distribution of selected pectic and arabinogalactan protein (AGP) epitopes in the walls of root cells.
34440734	2	36	with	contact	249:255	arg1	NP					262:263	NP	262:263	NP	262:263	In such conditions, plants come into direct contact with NP.
34440734	6	37	theme	epitopes	822:829	arg1	distribution					756:767	distribution	756:767	distribution	756:767	The obtained results indicate that AuNP with different surface charges affects the presence and distribution of selected pectic and arabinogalactan protein (AGP) epitopes in the walls of root cells.
34440734	6	37	theme	epitopes	822:829	arg1	presence					743:750	presence	743:750	presence	743:750	The obtained results indicate that AuNP with different surface charges affects the presence and distribution of selected pectic and arabinogalactan protein (AGP) epitopes in the walls of root cells.
34440734	2	38	theme	such	208:211	arg1	conditions					213:222	such conditions	208:222	such conditions	208:222	In such conditions, plants come into direct contact with NP.
34440734	4	39	from	analyses	408:415	arg1	components					448:457	the chemical components	435:457	the chemical components of the cell walls of Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP)	435:564	Our studies concerned analyses of the changes in the chemical components of the cell walls of Hordeum vulgare L. roots that were grown in the presence of gold nanoparticles (AuNP).
34440734	6	40	from	presence	743:750	arg1	walls					838:842	the walls	834:842	the walls of root cells	834:856	The obtained results indicate that AuNP with different surface charges affects the presence and distribution of selected pectic and arabinogalactan protein (AGP) epitopes in the walls of root cells.
34440734	2	41	theme	direct	242:247	arg1	contact					249:255	direct contact	242:255	direct contact with NP	242:263	In such conditions, plants come into direct contact with NP.
34440734	6	42	theme	surface	715:721	arg1	charges					723:729	different surface charges	705:729	different surface charges	705:729	The obtained results indicate that AuNP with different surface charges affects the presence and distribution of selected pectic and arabinogalactan protein (AGP) epitopes in the walls of root cells.
34440734	6	43	theme	different	705:713	arg1	charges					723:729	different surface charges	705:729	different surface charges	705:729	The obtained results indicate that AuNP with different surface charges affects the presence and distribution of selected pectic and arabinogalactan protein (AGP) epitopes in the walls of root cells.
34440734	1	44	theme	different	126:134	arg1	industries					136:145	different industries	126:145	different industries	126:145	The increased use of nanoparticles (NP) in different industries inevitably results in their release into the environment.
34440734	1	45	from	use	97:99	arg1	industries					136:145	different industries	126:145	different industries	126:145	The increased use of nanoparticles (NP) in different industries inevitably results in their release into the environment.
34440734	3	46	from	uptake	286:291	arg1	processes					353:361	different developmental processes	329:361	different developmental processes	329:361	Knowledge about the uptake of NP by plants and their effect on different developmental processes is still insufficient.
34440734	0	47	theme	Barley	69:74	arg1	Roots					76:80	Barley Roots	69:80	Barley Roots	69:80	Gold Nanoparticles-Induced Modifications in Cell Wall Composition in Barley Roots.
34440734	6	48	with	AuNP	695:698	arg1	charges					723:729	different surface charges	705:729	different surface charges	705:729	The obtained results indicate that AuNP with different surface charges affects the presence and distribution of selected pectic and arabinogalactan protein (AGP) epitopes in the walls of root cells.
34458800	0	0	theme	HA	71:72	arg1	functions					74:82	HA functions	71:82	HA functions	71:82	Hyaluronan (HA)-inspired glycopolymers as molecular tools for studying HA functions.
34458800	8	1	theme	glycopolymers	1574:1586	arg1	effect					1561:1566	The inhibitory effect	1546:1566	The inhibitory effect of HA glycopolymers on hyaluronidase (HAase) activity	1546:1620	The inhibitory effect of HA glycopolymers on hyaluronidase (HAase) activity was also investigated suggesting GlcNAc- and GlcA-based glycopolymers as potential HAase inhibitors.
34458800	1	2	theme	non-sulphated	116:128	arg1	acid					96:99	Hyaluronic acid	85:99	Hyaluronic acid (HA)	85:104	Hyaluronic acid (HA), the only non-sulphated glycosaminoglycan, serves numerous structural and biological functions in the human body, from providing viscoelasticity in tissues to creating hydrated environments for cell migration and proliferation.
34458800	1	2	theme	non-sulphated	116:128	arg1	glycosaminoglycan					130:146	the only non-sulphated glycosaminoglycan	107:146	the only non-sulphated glycosaminoglycan	107:146	Hyaluronic acid (HA), the only non-sulphated glycosaminoglycan, serves numerous structural and biological functions in the human body, from providing viscoelasticity in tissues to creating hydrated environments for cell migration and proliferation.
34458800	5	3	theme	chain	959:963	arg1	polymerisation					974:987	reversible addition-fragmentation chain transfer polymerisation	925:987	reversible addition-fragmentation chain transfer polymerisation	925:987	A library of homo, statistical and alternating HA glycopolymers were synthesised by reversible addition-fragmentation chain transfer polymerisation and post-modification utilising copper alkyne-azide cycloaddition to graft orthogonal pendant HA monosaccharides (N-acetyl glucosamine: GlcNAc and glucuronic acid: GlcA) onto the polymer.
34458800	8	4	theme	HA	1571:1572	arg1	glycopolymers					1574:1586	HA glycopolymers	1571:1586	HA glycopolymers	1571:1586	The inhibitory effect of HA glycopolymers on hyaluronidase (HAase) activity was also investigated suggesting GlcNAc- and GlcA-based glycopolymers as potential HAase inhibitors.
34458800	8	5	theme	HAase	1705:1709	arg1	inhibitors					1711:1720	potential HAase inhibitors	1695:1720	potential HAase inhibitors	1695:1720	The inhibitory effect of HA glycopolymers on hyaluronidase (HAase) activity was also investigated suggesting GlcNAc- and GlcA-based glycopolymers as potential HAase inhibitors.
34458800	8	5	theme	HAase	1705:1709	arg1	glycopolymers					1678:1690	GlcNAc- and GlcA-based glycopolymers	1655:1690	GlcNAc- and GlcA-based glycopolymers	1655:1690	The inhibitory effect of HA glycopolymers on hyaluronidase (HAase) activity was also investigated suggesting GlcNAc- and GlcA-based glycopolymers as potential HAase inhibitors.
34458800	4	6	theme	precise	701:707	arg1	platform					719:726	a simple and precise synthetic platform	688:726	a simple and precise synthetic platform for the preparation of glycan analogues	688:766	Glycopolymers offer a simple and precise synthetic platform for the preparation of glycan analogues, being an alternative to the demanding synthetic chemical glycosylation.
34458800	8	7	theme	hyaluronidase	1591:1603	arg1	activity					1613:1620	hyaluronidase (HAase) activity	1591:1620	hyaluronidase (HAase) activity	1591:1620	The inhibitory effect of HA glycopolymers on hyaluronidase (HAase) activity was also investigated suggesting GlcNAc- and GlcA-based glycopolymers as potential HAase inhibitors.
34458800	5	8	theme	pendant	1075:1081	arg1	monosaccharides					1086:1100	graft orthogonal pendant HA monosaccharides	1058:1100	graft orthogonal pendant HA monosaccharides (N-acetyl glucosamine: GlcNAc and glucuronic acid: GlcA) onto the polymer	1058:1174	A library of homo, statistical and alternating HA glycopolymers were synthesised by reversible addition-fragmentation chain transfer polymerisation and post-modification utilising copper alkyne-azide cycloaddition to graft orthogonal pendant HA monosaccharides (N-acetyl glucosamine: GlcNAc and glucuronic acid: GlcA) onto the polymer.
34458800	6	9	theme	carbohydrate-binding	1336:1355	arg1	lectins					1367:1373	lectins	1367:1373	lectins	1367:1373	Using surface plasmon resonance, the binding of the glycopolymers to known HA-binding peptides and proteins (CD44, hyaluronidase) was assessed and compared to carbohydrate-binding proteins (lectins).
34458800	6	9	theme	carbohydrate-binding	1336:1355	arg1	proteins					1357:1364	carbohydrate-binding proteins	1336:1364	carbohydrate-binding proteins (lectins)	1336:1374	Using surface plasmon resonance, the binding of the glycopolymers to known HA-binding peptides and proteins (CD44, hyaluronidase) was assessed and compared to carbohydrate-binding proteins (lectins).
34458800	5	10	theme	alkyne-azide	1028:1039	arg1	cycloaddition					1041:1053	copper alkyne-azide cycloaddition	1021:1053	copper alkyne-azide cycloaddition	1021:1053	A library of homo, statistical and alternating HA glycopolymers were synthesised by reversible addition-fragmentation chain transfer polymerisation and post-modification utilising copper alkyne-azide cycloaddition to graft orthogonal pendant HA monosaccharides (N-acetyl glucosamine: GlcNAc and glucuronic acid: GlcA) onto the polymer.
34458800	5	11	theme	copper	1021:1026	arg1	cycloaddition					1041:1053	copper alkyne-azide cycloaddition	1021:1053	copper alkyne-azide cycloaddition	1021:1053	A library of homo, statistical and alternating HA glycopolymers were synthesised by reversible addition-fragmentation chain transfer polymerisation and post-modification utilising copper alkyne-azide cycloaddition to graft orthogonal pendant HA monosaccharides (N-acetyl glucosamine: GlcNAc and glucuronic acid: GlcA) onto the polymer.
34458800	6	12	theme	known	1246:1250	arg1	peptides					1263:1270	known HA-binding peptides	1246:1270	known HA-binding peptides	1246:1270	Using surface plasmon resonance, the binding of the glycopolymers to known HA-binding peptides and proteins (CD44, hyaluronidase) was assessed and compared to carbohydrate-binding proteins (lectins).
34458800	4	13	theme	synthetic	709:717	arg1	platform					719:726	a simple and precise synthetic platform	688:726	a simple and precise synthetic platform for the preparation of glycan analogues	688:766	Glycopolymers offer a simple and precise synthetic platform for the preparation of glycan analogues, being an alternative to the demanding synthetic chemical glycosylation.
34458800	6	14	theme	surface	1183:1189	arg1	resonance					1199:1207	surface plasmon resonance	1183:1207	surface plasmon resonance	1183:1207	Using surface plasmon resonance, the binding of the glycopolymers to known HA-binding peptides and proteins (CD44, hyaluronidase) was assessed and compared to carbohydrate-binding proteins (lectins).
34458800	8	15	from	effect	1561:1566	arg1	activity					1613:1620	hyaluronidase (HAase) activity	1591:1620	hyaluronidase (HAase) activity	1591:1620	The inhibitory effect of HA glycopolymers on hyaluronidase (HAase) activity was also investigated suggesting GlcNAc- and GlcA-based glycopolymers as potential HAase inhibitors.
34458800	8	16	theme	HAase	1606:1610	arg1	activity					1613:1620	hyaluronidase (HAase) activity	1591:1620	hyaluronidase (HAase) activity	1591:1620	The inhibitory effect of HA glycopolymers on hyaluronidase (HAase) activity was also investigated suggesting GlcNAc- and GlcA-based glycopolymers as potential HAase inhibitors.
34458800	5	17	theme	homo	854:857	arg1	library					843:849	A library	841:849	A library of homo, statistical and alternating HA glycopolymers	841:903	A library of homo, statistical and alternating HA glycopolymers were synthesised by reversible addition-fragmentation chain transfer polymerisation and post-modification utilising copper alkyne-azide cycloaddition to graft orthogonal pendant HA monosaccharides (N-acetyl glucosamine: GlcNAc and glucuronic acid: GlcA) onto the polymer.
34458800	5	18	theme	HA	888:889	arg1	glycopolymers					891:903	alternating HA glycopolymers	876:903	alternating HA glycopolymers	876:903	A library of homo, statistical and alternating HA glycopolymers were synthesised by reversible addition-fragmentation chain transfer polymerisation and post-modification utilising copper alkyne-azide cycloaddition to graft orthogonal pendant HA monosaccharides (N-acetyl glucosamine: GlcNAc and glucuronic acid: GlcA) onto the polymer.
34458800	2	19	theme	morphogenesis	375:387	arg1	regulation					361:370	the regulation	357:370	the regulation of morphogenesis, inflammation and tumorigenesis through interactions with specific HA-binding proteins	357:474	HA is also involved in the regulation of morphogenesis, inflammation and tumorigenesis through interactions with specific HA-binding proteins.
34458800	6	20	dep	proteins	1276:1283	arg1	hyaluronidase					1292:1304	hyaluronidase	1292:1304	hyaluronidase	1292:1304	Using surface plasmon resonance, the binding of the glycopolymers to known HA-binding peptides and proteins (CD44, hyaluronidase) was assessed and compared to carbohydrate-binding proteins (lectins).
34458800	6	20	dep	proteins	1276:1283	arg1	CD44					1286:1289	CD44	1286:1289	CD44	1286:1289	Using surface plasmon resonance, the binding of the glycopolymers to known HA-binding peptides and proteins (CD44, hyaluronidase) was assessed and compared to carbohydrate-binding proteins (lectins).
34458800	5	21	theme	reversible	925:934	arg1	polymerisation					974:987	reversible addition-fragmentation chain transfer polymerisation	925:987	reversible addition-fragmentation chain transfer polymerisation	925:987	A library of homo, statistical and alternating HA glycopolymers were synthesised by reversible addition-fragmentation chain transfer polymerisation and post-modification utilising copper alkyne-azide cycloaddition to graft orthogonal pendant HA monosaccharides (N-acetyl glucosamine: GlcNAc and glucuronic acid: GlcA) onto the polymer.
34458800	6	22	theme	plasmon	1191:1197	arg1	resonance					1199:1207	surface plasmon resonance	1183:1207	surface plasmon resonance	1183:1207	Using surface plasmon resonance, the binding of the glycopolymers to known HA-binding peptides and proteins (CD44, hyaluronidase) was assessed and compared to carbohydrate-binding proteins (lectins).
34458800	5	23	theme	graft	1058:1062	arg1	monosaccharides					1086:1100	graft orthogonal pendant HA monosaccharides	1058:1100	graft orthogonal pendant HA monosaccharides (N-acetyl glucosamine: GlcNAc and glucuronic acid: GlcA) onto the polymer	1058:1174	A library of homo, statistical and alternating HA glycopolymers were synthesised by reversible addition-fragmentation chain transfer polymerisation and post-modification utilising copper alkyne-azide cycloaddition to graft orthogonal pendant HA monosaccharides (N-acetyl glucosamine: GlcNAc and glucuronic acid: GlcA) onto the polymer.
34458800	8	24	theme	inhibitory	1550:1559	arg1	effect					1561:1566	The inhibitory effect	1546:1566	The inhibitory effect of HA glycopolymers on hyaluronidase (HAase) activity	1546:1620	The inhibitory effect of HA glycopolymers on hyaluronidase (HAase) activity was also investigated suggesting GlcNAc- and GlcA-based glycopolymers as potential HAase inhibitors.
34458800	5	25	theme	alternating	876:886	arg1	glycopolymers					891:903	alternating HA glycopolymers	876:903	alternating HA glycopolymers	876:903	A library of homo, statistical and alternating HA glycopolymers were synthesised by reversible addition-fragmentation chain transfer polymerisation and post-modification utilising copper alkyne-azide cycloaddition to graft orthogonal pendant HA monosaccharides (N-acetyl glucosamine: GlcNAc and glucuronic acid: GlcA) onto the polymer.
34458800	2	26	theme	specific	447:454	arg1	proteins					467:474	specific HA-binding proteins	447:474	specific HA-binding proteins	447:474	HA is also involved in the regulation of morphogenesis, inflammation and tumorigenesis through interactions with specific HA-binding proteins.
34458800	4	27	theme	glycan	751:756	arg1	analogues					758:766	glycan analogues	751:766	glycan analogues	751:766	Glycopolymers offer a simple and precise synthetic platform for the preparation of glycan analogues, being an alternative to the demanding synthetic chemical glycosylation.
34458800	1	28	theme	numerous	156:163	arg1	functions					191:199	numerous structural and biological functions	156:199	numerous structural and biological functions in the human body	156:217	Hyaluronic acid (HA), the only non-sulphated glycosaminoglycan, serves numerous structural and biological functions in the human body, from providing viscoelasticity in tissues to creating hydrated environments for cell migration and proliferation.
34458800	7	29	theme	Dectin-1	1516:1523	arg1	lectins					1537:1543	Dectin-1 and DEC-205 lectins	1516:1543	Dectin-1 and DEC-205 lectins	1516:1543	These studies revealed potential structure-binding relationships between HA monosaccharides and HA receptors and novel HA binders, such as Dectin-1 and DEC-205 lectins.
34458800	3	30	theme	exact	578:582	arg1	mechanisms					584:593	the exact mechanisms	574:593	the exact mechanisms by which HA exerts its multiple functions	574:635	Whilst the physicochemical and biological properties of HA have been widely studied for decades, the exact mechanisms by which HA exerts its multiple functions are not completely understood.
34458800	5	31	dep	monosaccharides	1086:1100	arg1	glucosamine					1112:1122	N-acetyl glucosamine	1103:1122	N-acetyl glucosamine: GlcNAc and glucuronic acid: GlcA	1103:1156	A library of homo, statistical and alternating HA glycopolymers were synthesised by reversible addition-fragmentation chain transfer polymerisation and post-modification utilising copper alkyne-azide cycloaddition to graft orthogonal pendant HA monosaccharides (N-acetyl glucosamine: GlcNAc and glucuronic acid: GlcA) onto the polymer.
34458800	1	32	theme	structural	165:174	arg1	functions					191:199	numerous structural and biological functions	156:199	numerous structural and biological functions in the human body	156:217	Hyaluronic acid (HA), the only non-sulphated glycosaminoglycan, serves numerous structural and biological functions in the human body, from providing viscoelasticity in tissues to creating hydrated environments for cell migration and proliferation.
34458800	5	33	theme	HA	1083:1084	arg1	monosaccharides					1086:1100	graft orthogonal pendant HA monosaccharides	1058:1100	graft orthogonal pendant HA monosaccharides (N-acetyl glucosamine: GlcNAc and glucuronic acid: GlcA) onto the polymer	1058:1174	A library of homo, statistical and alternating HA glycopolymers were synthesised by reversible addition-fragmentation chain transfer polymerisation and post-modification utilising copper alkyne-azide cycloaddition to graft orthogonal pendant HA monosaccharides (N-acetyl glucosamine: GlcNAc and glucuronic acid: GlcA) onto the polymer.
34458800	1	34	from	viscoelasticity	235:249	arg1	tissues					254:260	tissues	254:260	tissues	254:260	Hyaluronic acid (HA), the only non-sulphated glycosaminoglycan, serves numerous structural and biological functions in the human body, from providing viscoelasticity in tissues to creating hydrated environments for cell migration and proliferation.
34458800	1	35	theme	Hyaluronic	85:94	arg1	glycosaminoglycan					130:146	the only non-sulphated glycosaminoglycan	107:146	the only non-sulphated glycosaminoglycan	107:146	Hyaluronic acid (HA), the only non-sulphated glycosaminoglycan, serves numerous structural and biological functions in the human body, from providing viscoelasticity in tissues to creating hydrated environments for cell migration and proliferation.
34458800	1	35	theme	Hyaluronic	85:94	arg1	HA					102:103	HA	102:103	HA	102:103	Hyaluronic acid (HA), the only non-sulphated glycosaminoglycan, serves numerous structural and biological functions in the human body, from providing viscoelasticity in tissues to creating hydrated environments for cell migration and proliferation.
34458800	1	35	theme	Hyaluronic	85:94	arg1	acid					96:99	Hyaluronic acid	85:99	Hyaluronic acid (HA)	85:104	Hyaluronic acid (HA), the only non-sulphated glycosaminoglycan, serves numerous structural and biological functions in the human body, from providing viscoelasticity in tissues to creating hydrated environments for cell migration and proliferation.
34458800	2	36	theme	tumorigenesis	407:419	arg1	regulation					361:370	the regulation	357:370	the regulation of morphogenesis, inflammation and tumorigenesis through interactions with specific HA-binding proteins	357:474	HA is also involved in the regulation of morphogenesis, inflammation and tumorigenesis through interactions with specific HA-binding proteins.
34458800	1	37	theme	creating	265:272	arg1	environments					283:294	creating hydrated environments	265:294	creating hydrated environments	265:294	Hyaluronic acid (HA), the only non-sulphated glycosaminoglycan, serves numerous structural and biological functions in the human body, from providing viscoelasticity in tissues to creating hydrated environments for cell migration and proliferation.
34458800	6	38	theme	HA-binding	1252:1261	arg1	peptides					1263:1270	known HA-binding peptides	1246:1270	known HA-binding peptides	1246:1270	Using surface plasmon resonance, the binding of the glycopolymers to known HA-binding peptides and proteins (CD44, hyaluronidase) was assessed and compared to carbohydrate-binding proteins (lectins).
34458800	4	39	theme	analogues	758:766	arg1	preparation					736:746	the preparation	732:746	the preparation of glycan analogues	732:766	Glycopolymers offer a simple and precise synthetic platform for the preparation of glycan analogues, being an alternative to the demanding synthetic chemical glycosylation.
34458800	8	40	theme	GlcNAc-	1655:1661	arg1	inhibitors					1711:1720	potential HAase inhibitors	1695:1720	potential HAase inhibitors	1695:1720	The inhibitory effect of HA glycopolymers on hyaluronidase (HAase) activity was also investigated suggesting GlcNAc- and GlcA-based glycopolymers as potential HAase inhibitors.
34458800	8	40	theme	GlcNAc-	1655:1661	arg1	glycopolymers					1678:1690	GlcNAc- and GlcA-based glycopolymers	1655:1690	GlcNAc- and GlcA-based glycopolymers	1655:1690	The inhibitory effect of HA glycopolymers on hyaluronidase (HAase) activity was also investigated suggesting GlcNAc- and GlcA-based glycopolymers as potential HAase inhibitors.
34458800	1	41	theme	biological	180:189	arg1	functions					191:199	numerous structural and biological functions	156:199	numerous structural and biological functions in the human body	156:217	Hyaluronic acid (HA), the only non-sulphated glycosaminoglycan, serves numerous structural and biological functions in the human body, from providing viscoelasticity in tissues to creating hydrated environments for cell migration and proliferation.
34458800	1	42	theme	hydrated	274:281	arg1	environments					283:294	creating hydrated environments	265:294	creating hydrated environments	265:294	Hyaluronic acid (HA), the only non-sulphated glycosaminoglycan, serves numerous structural and biological functions in the human body, from providing viscoelasticity in tissues to creating hydrated environments for cell migration and proliferation.
34458800	5	43	theme	glycopolymers	891:903	arg1	library					843:849	A library	841:849	A library of homo, statistical and alternating HA glycopolymers	841:903	A library of homo, statistical and alternating HA glycopolymers were synthesised by reversible addition-fragmentation chain transfer polymerisation and post-modification utilising copper alkyne-azide cycloaddition to graft orthogonal pendant HA monosaccharides (N-acetyl glucosamine: GlcNAc and glucuronic acid: GlcA) onto the polymer.
34458800	2	44	theme	inflammation	390:401	arg1	regulation					361:370	the regulation	357:370	the regulation of morphogenesis, inflammation and tumorigenesis through interactions with specific HA-binding proteins	357:474	HA is also involved in the regulation of morphogenesis, inflammation and tumorigenesis through interactions with specific HA-binding proteins.
34458800	5	45	dep	glucosamine	1112:1122	arg1	GlcA					1153:1156	GlcA	1153:1156	GlcA	1153:1156	A library of homo, statistical and alternating HA glycopolymers were synthesised by reversible addition-fragmentation chain transfer polymerisation and post-modification utilising copper alkyne-azide cycloaddition to graft orthogonal pendant HA monosaccharides (N-acetyl glucosamine: GlcNAc and glucuronic acid: GlcA) onto the polymer.
34458800	5	45	dep	glucosamine	1112:1122	arg1	GlcNAc					1125:1130	GlcNAc	1125:1130	GlcNAc	1125:1130	A library of homo, statistical and alternating HA glycopolymers were synthesised by reversible addition-fragmentation chain transfer polymerisation and post-modification utilising copper alkyne-azide cycloaddition to graft orthogonal pendant HA monosaccharides (N-acetyl glucosamine: GlcNAc and glucuronic acid: GlcA) onto the polymer.
34458800	5	45	dep	glucosamine	1112:1122	arg1	acid					1147:1150	glucuronic acid	1136:1150	glucuronic acid	1136:1150	A library of homo, statistical and alternating HA glycopolymers were synthesised by reversible addition-fragmentation chain transfer polymerisation and post-modification utilising copper alkyne-azide cycloaddition to graft orthogonal pendant HA monosaccharides (N-acetyl glucosamine: GlcNAc and glucuronic acid: GlcA) onto the polymer.
34458800	8	46	theme	GlcA-based	1667:1676	arg1	inhibitors					1711:1720	potential HAase inhibitors	1695:1720	potential HAase inhibitors	1695:1720	The inhibitory effect of HA glycopolymers on hyaluronidase (HAase) activity was also investigated suggesting GlcNAc- and GlcA-based glycopolymers as potential HAase inhibitors.
34458800	8	46	theme	GlcA-based	1667:1676	arg1	glycopolymers					1678:1690	GlcNAc- and GlcA-based glycopolymers	1655:1690	GlcNAc- and GlcA-based glycopolymers	1655:1690	The inhibitory effect of HA glycopolymers on hyaluronidase (HAase) activity was also investigated suggesting GlcNAc- and GlcA-based glycopolymers as potential HAase inhibitors.
34458800	7	47	theme	potential	1400:1408	arg1	relationships					1428:1440	potential structure-binding relationships	1400:1440	potential structure-binding relationships between HA monosaccharides and HA receptors and novel HA binders, such as Dectin-1 and DEC-205 lectins	1400:1543	These studies revealed potential structure-binding relationships between HA monosaccharides and HA receptors and novel HA binders, such as Dectin-1 and DEC-205 lectins.
34458800	5	48	theme	N-acetyl	1103:1110	arg1	glucosamine					1112:1122	N-acetyl glucosamine	1103:1122	N-acetyl glucosamine: GlcNAc and glucuronic acid: GlcA	1103:1156	A library of homo, statistical and alternating HA glycopolymers were synthesised by reversible addition-fragmentation chain transfer polymerisation and post-modification utilising copper alkyne-azide cycloaddition to graft orthogonal pendant HA monosaccharides (N-acetyl glucosamine: GlcNAc and glucuronic acid: GlcA) onto the polymer.
34458800	2	49	with	interactions	429:440	arg1	proteins					467:474	specific HA-binding proteins	447:474	specific HA-binding proteins	447:474	HA is also involved in the regulation of morphogenesis, inflammation and tumorigenesis through interactions with specific HA-binding proteins.
34458800	5	50	theme	statistical	860:870	arg1	library					843:849	A library	841:849	A library of homo, statistical and alternating HA glycopolymers	841:903	A library of homo, statistical and alternating HA glycopolymers were synthesised by reversible addition-fragmentation chain transfer polymerisation and post-modification utilising copper alkyne-azide cycloaddition to graft orthogonal pendant HA monosaccharides (N-acetyl glucosamine: GlcNAc and glucuronic acid: GlcA) onto the polymer.
34458800	0	51	theme	molecular	42:50	arg1	glycopolymers					25:37	glycopolymers	25:37	glycopolymers	25:37	Hyaluronan (HA)-inspired glycopolymers as molecular tools for studying HA functions.
34458800	0	51	theme	molecular	42:50	arg1	tools					52:56	molecular tools	42:56	molecular tools for studying HA functions	42:82	Hyaluronan (HA)-inspired glycopolymers as molecular tools for studying HA functions.
34458800	8	52	theme	potential	1695:1703	arg1	inhibitors					1711:1720	potential HAase inhibitors	1695:1720	potential HAase inhibitors	1695:1720	The inhibitory effect of HA glycopolymers on hyaluronidase (HAase) activity was also investigated suggesting GlcNAc- and GlcA-based glycopolymers as potential HAase inhibitors.
34458800	8	52	theme	potential	1695:1703	arg1	glycopolymers					1678:1690	GlcNAc- and GlcA-based glycopolymers	1655:1690	GlcNAc- and GlcA-based glycopolymers	1655:1690	The inhibitory effect of HA glycopolymers on hyaluronidase (HAase) activity was also investigated suggesting GlcNAc- and GlcA-based glycopolymers as potential HAase inhibitors.
34458800	7	53	theme	DEC-205	1529:1535	arg1	lectins					1537:1543	Dectin-1 and DEC-205 lectins	1516:1543	Dectin-1 and DEC-205 lectins	1516:1543	These studies revealed potential structure-binding relationships between HA monosaccharides and HA receptors and novel HA binders, such as Dectin-1 and DEC-205 lectins.
34458800	5	54	theme	addition-fragmentation	936:957	arg1	polymerisation					974:987	reversible addition-fragmentation chain transfer polymerisation	925:987	reversible addition-fragmentation chain transfer polymerisation	925:987	A library of homo, statistical and alternating HA glycopolymers were synthesised by reversible addition-fragmentation chain transfer polymerisation and post-modification utilising copper alkyne-azide cycloaddition to graft orthogonal pendant HA monosaccharides (N-acetyl glucosamine: GlcNAc and glucuronic acid: GlcA) onto the polymer.
34458800	2	55	theme	HA-binding	456:465	arg1	proteins					467:474	specific HA-binding proteins	447:474	specific HA-binding proteins	447:474	HA is also involved in the regulation of morphogenesis, inflammation and tumorigenesis through interactions with specific HA-binding proteins.
34458800	1	56	theme	cell	300:303	arg1	migration					305:313	cell migration	300:313	cell migration	300:313	Hyaluronic acid (HA), the only non-sulphated glycosaminoglycan, serves numerous structural and biological functions in the human body, from providing viscoelasticity in tissues to creating hydrated environments for cell migration and proliferation.
34458800	7	57	theme	HA	1473:1474	arg1	receptors					1476:1484	HA receptors	1473:1484	HA receptors	1473:1484	These studies revealed potential structure-binding relationships between HA monosaccharides and HA receptors and novel HA binders, such as Dectin-1 and DEC-205 lectins.
34458800	7	57	theme	HA	1473:1474	arg1	lectins					1537:1543	Dectin-1 and DEC-205 lectins	1516:1543	Dectin-1 and DEC-205 lectins	1516:1543	These studies revealed potential structure-binding relationships between HA monosaccharides and HA receptors and novel HA binders, such as Dectin-1 and DEC-205 lectins.
34458800	3	58	theme	biological	508:517	arg1	properties					519:528	the physicochemical and biological properties	484:528	the physicochemical and biological properties of HA	484:534	Whilst the physicochemical and biological properties of HA have been widely studied for decades, the exact mechanisms by which HA exerts its multiple functions are not completely understood.
34458800	4	59	theme	demanding	797:805	arg1	glycosylation					826:838	the demanding synthetic chemical glycosylation	793:838	the demanding synthetic chemical glycosylation	793:838	Glycopolymers offer a simple and precise synthetic platform for the preparation of glycan analogues, being an alternative to the demanding synthetic chemical glycosylation.
34458800	3	60	theme	multiple	618:625	arg1	functions					627:635	its multiple functions	614:635	its multiple functions	614:635	Whilst the physicochemical and biological properties of HA have been widely studied for decades, the exact mechanisms by which HA exerts its multiple functions are not completely understood.
34458800	4	61	theme	simple	690:695	arg1	platform					719:726	a simple and precise synthetic platform	688:726	a simple and precise synthetic platform for the preparation of glycan analogues	688:766	Glycopolymers offer a simple and precise synthetic platform for the preparation of glycan analogues, being an alternative to the demanding synthetic chemical glycosylation.
34458800	5	62	theme	transfer	965:972	arg1	polymerisation					974:987	reversible addition-fragmentation chain transfer polymerisation	925:987	reversible addition-fragmentation chain transfer polymerisation	925:987	A library of homo, statistical and alternating HA glycopolymers were synthesised by reversible addition-fragmentation chain transfer polymerisation and post-modification utilising copper alkyne-azide cycloaddition to graft orthogonal pendant HA monosaccharides (N-acetyl glucosamine: GlcNAc and glucuronic acid: GlcA) onto the polymer.
34458800	7	63	theme	novel	1490:1494	arg1	binders					1499:1505	novel HA binders	1490:1505	novel HA binders	1490:1505	These studies revealed potential structure-binding relationships between HA monosaccharides and HA receptors and novel HA binders, such as Dectin-1 and DEC-205 lectins.
34458800	7	63	theme	novel	1490:1494	arg1	lectins					1537:1543	Dectin-1 and DEC-205 lectins	1516:1543	Dectin-1 and DEC-205 lectins	1516:1543	These studies revealed potential structure-binding relationships between HA monosaccharides and HA receptors and novel HA binders, such as Dectin-1 and DEC-205 lectins.
34458800	7	64	theme	HA	1496:1497	arg1	binders					1499:1505	novel HA binders	1490:1505	novel HA binders	1490:1505	These studies revealed potential structure-binding relationships between HA monosaccharides and HA receptors and novel HA binders, such as Dectin-1 and DEC-205 lectins.
34458800	7	64	theme	HA	1496:1497	arg1	lectins					1537:1543	Dectin-1 and DEC-205 lectins	1516:1543	Dectin-1 and DEC-205 lectins	1516:1543	These studies revealed potential structure-binding relationships between HA monosaccharides and HA receptors and novel HA binders, such as Dectin-1 and DEC-205 lectins.
34458800	6	65	theme	glycopolymers	1229:1241	arg1	binding					1214:1220	the binding	1210:1220	the binding of the glycopolymers to known HA-binding peptides and proteins (CD44, hyaluronidase)	1210:1305	Using surface plasmon resonance, the binding of the glycopolymers to known HA-binding peptides and proteins (CD44, hyaluronidase) was assessed and compared to carbohydrate-binding proteins (lectins).
34458800	5	66	theme	glucuronic	1136:1145	arg1	acid					1147:1150	glucuronic acid	1136:1150	glucuronic acid	1136:1150	A library of homo, statistical and alternating HA glycopolymers were synthesised by reversible addition-fragmentation chain transfer polymerisation and post-modification utilising copper alkyne-azide cycloaddition to graft orthogonal pendant HA monosaccharides (N-acetyl glucosamine: GlcNAc and glucuronic acid: GlcA) onto the polymer.
34458800	1	67	from	functions	191:199	arg1	body					214:217	the human body	204:217	the human body	204:217	Hyaluronic acid (HA), the only non-sulphated glycosaminoglycan, serves numerous structural and biological functions in the human body, from providing viscoelasticity in tissues to creating hydrated environments for cell migration and proliferation.
34458800	3	68	theme	physicochemical	488:502	arg1	properties					519:528	the physicochemical and biological properties	484:528	the physicochemical and biological properties of HA	484:534	Whilst the physicochemical and biological properties of HA have been widely studied for decades, the exact mechanisms by which HA exerts its multiple functions are not completely understood.
34458800	1	69	theme	human	208:212	arg1	body					214:217	the human body	204:217	the human body	204:217	Hyaluronic acid (HA), the only non-sulphated glycosaminoglycan, serves numerous structural and biological functions in the human body, from providing viscoelasticity in tissues to creating hydrated environments for cell migration and proliferation.
34458800	4	70	theme	chemical	817:824	arg1	glycosylation					826:838	the demanding synthetic chemical glycosylation	793:838	the demanding synthetic chemical glycosylation	793:838	Glycopolymers offer a simple and precise synthetic platform for the preparation of glycan analogues, being an alternative to the demanding synthetic chemical glycosylation.
34458800	3	71	theme	HA	533:534	arg1	properties					519:528	the physicochemical and biological properties	484:528	the physicochemical and biological properties of HA	484:534	Whilst the physicochemical and biological properties of HA have been widely studied for decades, the exact mechanisms by which HA exerts its multiple functions are not completely understood.
34458800	7	72	theme	structure-binding	1410:1426	arg1	relationships					1428:1440	potential structure-binding relationships	1400:1440	potential structure-binding relationships between HA monosaccharides and HA receptors and novel HA binders, such as Dectin-1 and DEC-205 lectins	1400:1543	These studies revealed potential structure-binding relationships between HA monosaccharides and HA receptors and novel HA binders, such as Dectin-1 and DEC-205 lectins.
34458800	5	73	theme	orthogonal	1064:1073	arg1	monosaccharides					1086:1100	graft orthogonal pendant HA monosaccharides	1058:1100	graft orthogonal pendant HA monosaccharides (N-acetyl glucosamine: GlcNAc and glucuronic acid: GlcA) onto the polymer	1058:1174	A library of homo, statistical and alternating HA glycopolymers were synthesised by reversible addition-fragmentation chain transfer polymerisation and post-modification utilising copper alkyne-azide cycloaddition to graft orthogonal pendant HA monosaccharides (N-acetyl glucosamine: GlcNAc and glucuronic acid: GlcA) onto the polymer.
34458800	7	74	theme	HA	1450:1451	arg1	monosaccharides					1453:1467	HA monosaccharides	1450:1467	HA monosaccharides	1450:1467	These studies revealed potential structure-binding relationships between HA monosaccharides and HA receptors and novel HA binders, such as Dectin-1 and DEC-205 lectins.
34458800	7	74	theme	HA	1450:1451	arg1	lectins					1537:1543	Dectin-1 and DEC-205 lectins	1516:1543	Dectin-1 and DEC-205 lectins	1516:1543	These studies revealed potential structure-binding relationships between HA monosaccharides and HA receptors and novel HA binders, such as Dectin-1 and DEC-205 lectins.
34458800	4	75	theme	synthetic	807:815	arg1	glycosylation					826:838	the demanding synthetic chemical glycosylation	793:838	the demanding synthetic chemical glycosylation	793:838	Glycopolymers offer a simple and precise synthetic platform for the preparation of glycan analogues, being an alternative to the demanding synthetic chemical glycosylation.
34458800	1	76	theme	only	111:114	arg1	acid					96:99	Hyaluronic acid	85:99	Hyaluronic acid (HA)	85:104	Hyaluronic acid (HA), the only non-sulphated glycosaminoglycan, serves numerous structural and biological functions in the human body, from providing viscoelasticity in tissues to creating hydrated environments for cell migration and proliferation.
34458800	1	76	theme	only	111:114	arg1	glycosaminoglycan					130:146	the only non-sulphated glycosaminoglycan	107:146	the only non-sulphated glycosaminoglycan	107:146	Hyaluronic acid (HA), the only non-sulphated glycosaminoglycan, serves numerous structural and biological functions in the human body, from providing viscoelasticity in tissues to creating hydrated environments for cell migration and proliferation.
32479948	0	0	theme	water	109:113	arg1	retention					115:123	water retention	109:123	water retention	109:123	Synthesis and characterization of dopamine-modified Ca-alginate/poly(N-isopropylacrylamide) microspheres for water retention and multi-responsive controlled release of agrochemicals.
32479948	6	1	dep	water	1487:1491	arg1	material					1538:1545	controlled-release carrier material	1511:1545	controlled-release carrier material	1511:1545	Consequently, the Ca-alginate/PNIPAm@PDA microsphere with outstanding water-retention and controlled-release capacities is economical and eco-friendly and thus is promising for utilization as water and agrochemicals controlled-release carrier material in agriculture applications.
32479948	6	2	theme	carrier	1530:1536	arg1	material					1538:1545	controlled-release carrier material	1511:1545	controlled-release carrier material	1511:1545	Consequently, the Ca-alginate/PNIPAm@PDA microsphere with outstanding water-retention and controlled-release capacities is economical and eco-friendly and thus is promising for utilization as water and agrochemicals controlled-release carrier material in agriculture applications.
32479948	3	3	theme	scanning	841:848	arg1	microscopy					859:868	scanning electron microscopy	841:868	scanning electron microscopy	841:868	Microsphere with a semi-interpenetrating network, which contained pH-sensitive Ca-alginate, temperature-sensitive poly(N-isopropylacrylamide) (PNIPAm), and sunlight-sensitive polydopamine (PDA), was characterized by thermogravimetric analysis, zeta potential, Fourier transform infrared spectroscopy, and scanning electron microscopy to prove the successful synthesis.
32479948	1	4	theme	controlled-release	204:221	arg1	system					223:228	The multi-responsive controlled-release system	183:228	The multi-responsive controlled-release system	183:228	The multi-responsive controlled-release system could enhance crop yield while improving utilization efficiency of agrochemicals, and minimize environmental pollution caused by agrochemicals overuse.
32479948	5	5	theme	external	1273:1280	arg1	stimulation					1282:1292	external stimulation	1273:1292	external stimulation	1273:1292	The results indicated that the composite microsphere was a prosperous water and agrochemicals manager to effectively retain water and control the release of agrochemicals by external stimulation.
32479948	6	6	from	utilization	1472:1482	arg1	applications					1562:1573	agriculture applications	1550:1573	agriculture applications	1550:1573	Consequently, the Ca-alginate/PNIPAm@PDA microsphere with outstanding water-retention and controlled-release capacities is economical and eco-friendly and thus is promising for utilization as water and agrochemicals controlled-release carrier material in agriculture applications.
32479948	6	7	theme	controlled-release	1511:1528	arg1	material					1538:1545	controlled-release carrier material	1511:1545	controlled-release carrier material	1511:1545	Consequently, the Ca-alginate/PNIPAm@PDA microsphere with outstanding water-retention and controlled-release capacities is economical and eco-friendly and thus is promising for utilization as water and agrochemicals controlled-release carrier material in agriculture applications.
32479948	1	8	theme	environmental	325:337	arg1	pollution					339:347	environmental pollution	325:347	environmental pollution caused by agrochemicals overuse	325:379	The multi-responsive controlled-release system could enhance crop yield while improving utilization efficiency of agrochemicals, and minimize environmental pollution caused by agrochemicals overuse.
32479948	3	9	theme	infrared	814:821	arg1	spectroscopy					823:834	infrared spectroscopy	814:834	infrared spectroscopy	814:834	Microsphere with a semi-interpenetrating network, which contained pH-sensitive Ca-alginate, temperature-sensitive poly(N-isopropylacrylamide) (PNIPAm), and sunlight-sensitive polydopamine (PDA), was characterized by thermogravimetric analysis, zeta potential, Fourier transform infrared spectroscopy, and scanning electron microscopy to prove the successful synthesis.
32479948	4	10	theme	controlled-release	1021:1038	arg1	agrochemicals					1040:1052	controlled-release agrochemicals	1021:1052	controlled-release agrochemicals	1021:1052	Moreover, the comprehensive performances, including photothermal conversion, water absorbency, water retention, and controlled-release agrochemicals behaviors, were systematically investigated.
32479948	5	11	theme	composite	1130:1138	arg1	microsphere					1140:1150	the composite microsphere	1126:1150	the composite microsphere	1126:1150	The results indicated that the composite microsphere was a prosperous water and agrochemicals manager to effectively retain water and control the release of agrochemicals by external stimulation.
32479948	5	11	theme	composite	1130:1138	arg1	manager					1193:1199	a prosperous water and agrochemicals manager	1156:1199	a prosperous water and agrochemicals manager	1156:1199	The results indicated that the composite microsphere was a prosperous water and agrochemicals manager to effectively retain water and control the release of agrochemicals by external stimulation.
32479948	3	12	theme	electron	850:857	arg1	microscopy					859:868	scanning electron microscopy	841:868	scanning electron microscopy	841:868	Microsphere with a semi-interpenetrating network, which contained pH-sensitive Ca-alginate, temperature-sensitive poly(N-isopropylacrylamide) (PNIPAm), and sunlight-sensitive polydopamine (PDA), was characterized by thermogravimetric analysis, zeta potential, Fourier transform infrared spectroscopy, and scanning electron microscopy to prove the successful synthesis.
32479948	6	13	theme	controlled-release	1385:1402	arg1	capacities					1404:1413	outstanding water-retention and controlled-release capacities	1353:1413	outstanding water-retention and controlled-release capacities	1353:1413	Consequently, the Ca-alginate/PNIPAm@PDA microsphere with outstanding water-retention and controlled-release capacities is economical and eco-friendly and thus is promising for utilization as water and agrochemicals controlled-release carrier material in agriculture applications.
32479948	2	14	theme	@	447:447	arg1	PDA					481:483	Ca-alginate/PNIPAm@PDA	462:483	Ca-alginate/PNIPAm@PDA	462:483	This work reports a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere to control the agrochemicals release.
32479948	2	14	theme	@	447:447	arg1	microsphere					486:496	a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere	400:496	a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere to control the agrochemicals release	400:533	This work reports a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere to control the agrochemicals release.
32479948	2	15	theme	novel	402:406	arg1	PDA					481:483	Ca-alginate/PNIPAm@PDA	462:483	Ca-alginate/PNIPAm@PDA	462:483	This work reports a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere to control the agrochemicals release.
32479948	2	15	theme	novel	402:406	arg1	microsphere					486:496	a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere	400:496	a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere to control the agrochemicals release	400:533	This work reports a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere to control the agrochemicals release.
32479948	0	16	theme	controlled	146:155	arg1	release					157:163	multi-responsive controlled release	129:163	multi-responsive controlled release of agrochemicals	129:180	Synthesis and characterization of dopamine-modified Ca-alginate/poly(N-isopropylacrylamide) microspheres for water retention and multi-responsive controlled release of agrochemicals.
32479948	6	17	theme	@	1331:1331	arg1	microsphere					1336:1346	the Ca-alginate/PNIPAm@PDA microsphere	1309:1346	the Ca-alginate/PNIPAm@PDA microsphere with outstanding water-retention and controlled-release capacities	1309:1413	Consequently, the Ca-alginate/PNIPAm@PDA microsphere with outstanding water-retention and controlled-release capacities is economical and eco-friendly and thus is promising for utilization as water and agrochemicals controlled-release carrier material in agriculture applications.
32479948	6	17	theme	@	1331:1331	arg1	economical					1418:1427	economical	1418:1427	economical	1418:1427	Consequently, the Ca-alginate/PNIPAm@PDA microsphere with outstanding water-retention and controlled-release capacities is economical and eco-friendly and thus is promising for utilization as water and agrochemicals controlled-release carrier material in agriculture applications.
32479948	6	17	theme	@	1331:1331	arg1	promising					1458:1466	promising	1458:1466	promising	1458:1466	Consequently, the Ca-alginate/PNIPAm@PDA microsphere with outstanding water-retention and controlled-release capacities is economical and eco-friendly and thus is promising for utilization as water and agrochemicals controlled-release carrier material in agriculture applications.
32479948	3	18	dep	Fourier	796:802	arg1	transform					804:812	transform	804:812	transform infrared spectroscopy	804:834	Microsphere with a semi-interpenetrating network, which contained pH-sensitive Ca-alginate, temperature-sensitive poly(N-isopropylacrylamide) (PNIPAm), and sunlight-sensitive polydopamine (PDA), was characterized by thermogravimetric analysis, zeta potential, Fourier transform infrared spectroscopy, and scanning electron microscopy to prove the successful synthesis.
32479948	1	19	theme	crop	244:247	arg1	yield					249:253	crop yield	244:253	crop yield	244:253	The multi-responsive controlled-release system could enhance crop yield while improving utilization efficiency of agrochemicals, and minimize environmental pollution caused by agrochemicals overuse.
32479948	0	20	theme	multi-responsive	129:144	arg1	release					157:163	multi-responsive controlled release	129:163	multi-responsive controlled release of agrochemicals	129:180	Synthesis and characterization of dopamine-modified Ca-alginate/poly(N-isopropylacrylamide) microspheres for water retention and multi-responsive controlled release of agrochemicals.
32479948	4	21	theme	water	982:986	arg1	absorbency					988:997	water absorbency	982:997	water absorbency	982:997	Moreover, the comprehensive performances, including photothermal conversion, water absorbency, water retention, and controlled-release agrochemicals behaviors, were systematically investigated.
32479948	6	22	theme	water-retention	1365:1379	arg1	capacities					1404:1413	outstanding water-retention and controlled-release capacities	1353:1413	outstanding water-retention and controlled-release capacities	1353:1413	Consequently, the Ca-alginate/PNIPAm@PDA microsphere with outstanding water-retention and controlled-release capacities is economical and eco-friendly and thus is promising for utilization as water and agrochemicals controlled-release carrier material in agriculture applications.
32479948	3	23	contain	contained	592:600	arg2	N-isopropylacrylamide					655:675	N-isopropylacrylamide	655:675	N-isopropylacrylamide	655:675	Microsphere with a semi-interpenetrating network, which contained pH-sensitive Ca-alginate, temperature-sensitive poly(N-isopropylacrylamide) (PNIPAm), and sunlight-sensitive polydopamine (PDA), was characterized by thermogravimetric analysis, zeta potential, Fourier transform infrared spectroscopy, and scanning electron microscopy to prove the successful synthesis.
32479948	3	23	contain	contained	592:600	arg2	Ca-alginate					615:625	pH-sensitive Ca-alginate	602:625	pH-sensitive Ca-alginate	602:625	Microsphere with a semi-interpenetrating network, which contained pH-sensitive Ca-alginate, temperature-sensitive poly(N-isopropylacrylamide) (PNIPAm), and sunlight-sensitive polydopamine (PDA), was characterized by thermogravimetric analysis, zeta potential, Fourier transform infrared spectroscopy, and scanning electron microscopy to prove the successful synthesis.
32479948	3	23	contain	contained	592:600	arg2	polydopamine					711:722	sunlight-sensitive polydopamine	692:722	sunlight-sensitive polydopamine (PDA)	692:728	Microsphere with a semi-interpenetrating network, which contained pH-sensitive Ca-alginate, temperature-sensitive poly(N-isopropylacrylamide) (PNIPAm), and sunlight-sensitive polydopamine (PDA), was characterized by thermogravimetric analysis, zeta potential, Fourier transform infrared spectroscopy, and scanning electron microscopy to prove the successful synthesis.
32479948	3	23	contain	contained	592:600	arg2	poly					650:653	temperature-sensitive poly	628:653	temperature-sensitive poly(N-isopropylacrylamide) (PNIPAm)	628:685	Microsphere with a semi-interpenetrating network, which contained pH-sensitive Ca-alginate, temperature-sensitive poly(N-isopropylacrylamide) (PNIPAm), and sunlight-sensitive polydopamine (PDA), was characterized by thermogravimetric analysis, zeta potential, Fourier transform infrared spectroscopy, and scanning electron microscopy to prove the successful synthesis.
32479948	3	23	contain	contained	592:600	arg2	PDA					725:727	PDA	725:727	PDA	725:727	Microsphere with a semi-interpenetrating network, which contained pH-sensitive Ca-alginate, temperature-sensitive poly(N-isopropylacrylamide) (PNIPAm), and sunlight-sensitive polydopamine (PDA), was characterized by thermogravimetric analysis, zeta potential, Fourier transform infrared spectroscopy, and scanning electron microscopy to prove the successful synthesis.
32479948	3	23	contain	contained	592:600	arg2	PNIPAm					679:684	PNIPAm	679:684	PNIPAm	679:684	Microsphere with a semi-interpenetrating network, which contained pH-sensitive Ca-alginate, temperature-sensitive poly(N-isopropylacrylamide) (PNIPAm), and sunlight-sensitive polydopamine (PDA), was characterized by thermogravimetric analysis, zeta potential, Fourier transform infrared spectroscopy, and scanning electron microscopy to prove the successful synthesis.
32479948	3	23	contain	contained	592:600	arg1	network					577:583	a semi-interpenetrating network	553:583	a semi-interpenetrating network	553:583	Microsphere with a semi-interpenetrating network, which contained pH-sensitive Ca-alginate, temperature-sensitive poly(N-isopropylacrylamide) (PNIPAm), and sunlight-sensitive polydopamine (PDA), was characterized by thermogravimetric analysis, zeta potential, Fourier transform infrared spectroscopy, and scanning electron microscopy to prove the successful synthesis.
32479948	4	24	theme	water	1000:1004	arg1	retention					1006:1014	water retention	1000:1014	water retention	1000:1014	Moreover, the comprehensive performances, including photothermal conversion, water absorbency, water retention, and controlled-release agrochemicals behaviors, were systematically investigated.
32479948	6	25	theme	outstanding	1353:1363	arg1	capacities					1404:1413	outstanding water-retention and controlled-release capacities	1353:1413	outstanding water-retention and controlled-release capacities	1353:1413	Consequently, the Ca-alginate/PNIPAm@PDA microsphere with outstanding water-retention and controlled-release capacities is economical and eco-friendly and thus is promising for utilization as water and agrochemicals controlled-release carrier material in agriculture applications.
32479948	2	26	theme	@	480:480	arg1	PDA					481:483	Ca-alginate/PNIPAm@PDA	462:483	Ca-alginate/PNIPAm@PDA	462:483	This work reports a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere to control the agrochemicals release.
32479948	2	26	theme	@	480:480	arg1	microsphere					486:496	a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere	400:496	a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere to control the agrochemicals release	400:533	This work reports a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere to control the agrochemicals release.
32479948	2	27	theme	Ca-alginate/PNIPAm	462:479	arg1	PDA					481:483	Ca-alginate/PNIPAm@PDA	462:483	Ca-alginate/PNIPAm@PDA	462:483	This work reports a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere to control the agrochemicals release.
32479948	2	27	theme	Ca-alginate/PNIPAm	462:479	arg1	microsphere					486:496	a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere	400:496	a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere to control the agrochemicals release	400:533	This work reports a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere to control the agrochemicals release.
32479948	1	28	theme	agrochemicals	359:371	arg1	overuse					373:379	agrochemicals overuse	359:379	agrochemicals overuse	359:379	The multi-responsive controlled-release system could enhance crop yield while improving utilization efficiency of agrochemicals, and minimize environmental pollution caused by agrochemicals overuse.
32479948	3	29	theme	semi-interpenetrating	555:575	arg1	network					577:583	a semi-interpenetrating network	553:583	a semi-interpenetrating network	553:583	Microsphere with a semi-interpenetrating network, which contained pH-sensitive Ca-alginate, temperature-sensitive poly(N-isopropylacrylamide) (PNIPAm), and sunlight-sensitive polydopamine (PDA), was characterized by thermogravimetric analysis, zeta potential, Fourier transform infrared spectroscopy, and scanning electron microscopy to prove the successful synthesis.
32479948	2	30	theme	agrochemicals	513:525	arg1	release					527:533	the agrochemicals release	509:533	the agrochemicals release	509:533	This work reports a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere to control the agrochemicals release.
32479948	6	31	theme	PDA	1332:1334	arg1	microsphere					1336:1346	the Ca-alginate/PNIPAm@PDA microsphere	1309:1346	the Ca-alginate/PNIPAm@PDA microsphere with outstanding water-retention and controlled-release capacities	1309:1413	Consequently, the Ca-alginate/PNIPAm@PDA microsphere with outstanding water-retention and controlled-release capacities is economical and eco-friendly and thus is promising for utilization as water and agrochemicals controlled-release carrier material in agriculture applications.
32479948	6	31	theme	PDA	1332:1334	arg1	economical					1418:1427	economical	1418:1427	economical	1418:1427	Consequently, the Ca-alginate/PNIPAm@PDA microsphere with outstanding water-retention and controlled-release capacities is economical and eco-friendly and thus is promising for utilization as water and agrochemicals controlled-release carrier material in agriculture applications.
32479948	6	31	theme	PDA	1332:1334	arg1	promising					1458:1466	promising	1458:1466	promising	1458:1466	Consequently, the Ca-alginate/PNIPAm@PDA microsphere with outstanding water-retention and controlled-release capacities is economical and eco-friendly and thus is promising for utilization as water and agrochemicals controlled-release carrier material in agriculture applications.
32479948	4	32	dep	conversion	970:979	arg1	behaviors					1054:1062	behaviors	1054:1062	behaviors	1054:1062	Moreover, the comprehensive performances, including photothermal conversion, water absorbency, water retention, and controlled-release agrochemicals behaviors, were systematically investigated.
32479948	0	33	theme	Ca-alginate/poly	52:67	arg1	microspheres					92:103	dopamine-modified Ca-alginate/poly(N-isopropylacrylamide) microspheres	34:103	dopamine-modified Ca-alginate/poly(N-isopropylacrylamide) microspheres for water retention	34:123	Synthesis and characterization of dopamine-modified Ca-alginate/poly(N-isopropylacrylamide) microspheres for water retention and multi-responsive controlled release of agrochemicals.
32479948	6	34	theme	Ca-alginate/PNIPAm	1313:1330	arg1	microsphere					1336:1346	the Ca-alginate/PNIPAm@PDA microsphere	1309:1346	the Ca-alginate/PNIPAm@PDA microsphere with outstanding water-retention and controlled-release capacities	1309:1413	Consequently, the Ca-alginate/PNIPAm@PDA microsphere with outstanding water-retention and controlled-release capacities is economical and eco-friendly and thus is promising for utilization as water and agrochemicals controlled-release carrier material in agriculture applications.
32479948	6	34	theme	Ca-alginate/PNIPAm	1313:1330	arg1	economical					1418:1427	economical	1418:1427	economical	1418:1427	Consequently, the Ca-alginate/PNIPAm@PDA microsphere with outstanding water-retention and controlled-release capacities is economical and eco-friendly and thus is promising for utilization as water and agrochemicals controlled-release carrier material in agriculture applications.
32479948	6	34	theme	Ca-alginate/PNIPAm	1313:1330	arg1	promising					1458:1466	promising	1458:1466	promising	1458:1466	Consequently, the Ca-alginate/PNIPAm@PDA microsphere with outstanding water-retention and controlled-release capacities is economical and eco-friendly and thus is promising for utilization as water and agrochemicals controlled-release carrier material in agriculture applications.
32479948	4	35	theme	photothermal	957:968	arg1	conversion					970:979	photothermal conversion	957:979	photothermal conversion	957:979	Moreover, the comprehensive performances, including photothermal conversion, water absorbency, water retention, and controlled-release agrochemicals behaviors, were systematically investigated.
32479948	1	36	theme	utilization	271:281	arg1	efficiency					283:292	utilization efficiency	271:292	utilization efficiency of agrochemicals	271:309	The multi-responsive controlled-release system could enhance crop yield while improving utilization efficiency of agrochemicals, and minimize environmental pollution caused by agrochemicals overuse.
32479948	0	37	theme	dopamine-modified	34:50	arg1	microspheres					92:103	dopamine-modified Ca-alginate/poly(N-isopropylacrylamide) microspheres	34:103	dopamine-modified Ca-alginate/poly(N-isopropylacrylamide) microspheres for water retention	34:123	Synthesis and characterization of dopamine-modified Ca-alginate/poly(N-isopropylacrylamide) microspheres for water retention and multi-responsive controlled release of agrochemicals.
32479948	6	38	with	microsphere	1336:1346	arg1	capacities					1404:1413	outstanding water-retention and controlled-release capacities	1353:1413	outstanding water-retention and controlled-release capacities	1353:1413	Consequently, the Ca-alginate/PNIPAm@PDA microsphere with outstanding water-retention and controlled-release capacities is economical and eco-friendly and thus is promising for utilization as water and agrochemicals controlled-release carrier material in agriculture applications.
32479948	0	39	theme	agrochemicals	168:180	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Synthesis and characterization of dopamine-modified Ca-alginate/poly(N-isopropylacrylamide) microspheres for water retention and multi-responsive controlled release of agrochemicals.
32479948	0	39	theme	agrochemicals	168:180	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterization of dopamine-modified Ca-alginate/poly(N-isopropylacrylamide) microspheres for water retention and multi-responsive controlled release of agrochemicals.
32479948	0	39	theme	agrochemicals	168:180	arg1	release					157:163	multi-responsive controlled release	129:163	multi-responsive controlled release of agrochemicals	129:180	Synthesis and characterization of dopamine-modified Ca-alginate/poly(N-isopropylacrylamide) microspheres for water retention and multi-responsive controlled release of agrochemicals.
32479948	5	40	theme	agrochemicals	1256:1268	arg1	release					1245:1251	the release	1241:1251	the release of agrochemicals by external stimulation	1241:1292	The results indicated that the composite microsphere was a prosperous water and agrochemicals manager to effectively retain water and control the release of agrochemicals by external stimulation.
32479948	0	41	theme	N-isopropylacrylamide	69:89	arg1	microspheres					92:103	dopamine-modified Ca-alginate/poly(N-isopropylacrylamide) microspheres	34:103	dopamine-modified Ca-alginate/poly(N-isopropylacrylamide) microspheres for water retention	34:123	Synthesis and characterization of dopamine-modified Ca-alginate/poly(N-isopropylacrylamide) microspheres for water retention and multi-responsive controlled release of agrochemicals.
32479948	3	42	theme	thermogravimetric	752:768	arg1	analysis					770:777	thermogravimetric analysis	752:777	thermogravimetric analysis	752:777	Microsphere with a semi-interpenetrating network, which contained pH-sensitive Ca-alginate, temperature-sensitive poly(N-isopropylacrylamide) (PNIPAm), and sunlight-sensitive polydopamine (PDA), was characterized by thermogravimetric analysis, zeta potential, Fourier transform infrared spectroscopy, and scanning electron microscopy to prove the successful synthesis.
32479948	3	43	theme	sunlight-sensitive	692:709	arg1	PDA					725:727	PDA	725:727	PDA	725:727	Microsphere with a semi-interpenetrating network, which contained pH-sensitive Ca-alginate, temperature-sensitive poly(N-isopropylacrylamide) (PNIPAm), and sunlight-sensitive polydopamine (PDA), was characterized by thermogravimetric analysis, zeta potential, Fourier transform infrared spectroscopy, and scanning electron microscopy to prove the successful synthesis.
32479948	3	43	theme	sunlight-sensitive	692:709	arg1	polydopamine					711:722	sunlight-sensitive polydopamine	692:722	sunlight-sensitive polydopamine (PDA)	692:728	Microsphere with a semi-interpenetrating network, which contained pH-sensitive Ca-alginate, temperature-sensitive poly(N-isopropylacrylamide) (PNIPAm), and sunlight-sensitive polydopamine (PDA), was characterized by thermogravimetric analysis, zeta potential, Fourier transform infrared spectroscopy, and scanning electron microscopy to prove the successful synthesis.
32479948	2	44	theme	N-isopropylacrylamide	425:445	arg1	PDA					481:483	Ca-alginate/PNIPAm@PDA	462:483	Ca-alginate/PNIPAm@PDA	462:483	This work reports a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere to control the agrochemicals release.
32479948	2	44	theme	N-isopropylacrylamide	425:445	arg1	microsphere					486:496	a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere	400:496	a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere to control the agrochemicals release	400:533	This work reports a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere to control the agrochemicals release.
32479948	6	45	theme	agriculture	1550:1560	arg1	applications					1562:1573	agriculture applications	1550:1573	agriculture applications	1550:1573	Consequently, the Ca-alginate/PNIPAm@PDA microsphere with outstanding water-retention and controlled-release capacities is economical and eco-friendly and thus is promising for utilization as water and agrochemicals controlled-release carrier material in agriculture applications.
32479948	3	46	theme	pH-sensitive	602:613	arg1	Ca-alginate					615:625	pH-sensitive Ca-alginate	602:625	pH-sensitive Ca-alginate	602:625	Microsphere with a semi-interpenetrating network, which contained pH-sensitive Ca-alginate, temperature-sensitive poly(N-isopropylacrylamide) (PNIPAm), and sunlight-sensitive polydopamine (PDA), was characterized by thermogravimetric analysis, zeta potential, Fourier transform infrared spectroscopy, and scanning electron microscopy to prove the successful synthesis.
32479948	4	47	theme	comprehensive	919:931	arg1	performances					933:944	the comprehensive performances	915:944	the comprehensive performances	915:944	Moreover, the comprehensive performances, including photothermal conversion, water absorbency, water retention, and controlled-release agrochemicals behaviors, were systematically investigated.
32479948	4	47	theme	comprehensive	919:931	arg1	agrochemicals					1040:1052	controlled-release agrochemicals	1021:1052	controlled-release agrochemicals	1021:1052	Moreover, the comprehensive performances, including photothermal conversion, water absorbency, water retention, and controlled-release agrochemicals behaviors, were systematically investigated.
32479948	4	47	theme	comprehensive	919:931	arg1	conversion					970:979	photothermal conversion	957:979	photothermal conversion	957:979	Moreover, the comprehensive performances, including photothermal conversion, water absorbency, water retention, and controlled-release agrochemicals behaviors, were systematically investigated.
32479948	4	47	theme	comprehensive	919:931	arg1	absorbency					988:997	water absorbency	982:997	water absorbency	982:997	Moreover, the comprehensive performances, including photothermal conversion, water absorbency, water retention, and controlled-release agrochemicals behaviors, were systematically investigated.
32479948	4	47	theme	comprehensive	919:931	arg1	retention					1006:1014	water retention	1000:1014	water retention	1000:1014	Moreover, the comprehensive performances, including photothermal conversion, water absorbency, water retention, and controlled-release agrochemicals behaviors, were systematically investigated.
32479948	3	48	theme	successful	883:892	arg1	synthesis					894:902	the successful synthesis	879:902	the successful synthesis	879:902	Microsphere with a semi-interpenetrating network, which contained pH-sensitive Ca-alginate, temperature-sensitive poly(N-isopropylacrylamide) (PNIPAm), and sunlight-sensitive polydopamine (PDA), was characterized by thermogravimetric analysis, zeta potential, Fourier transform infrared spectroscopy, and scanning electron microscopy to prove the successful synthesis.
32479948	2	49	theme	Ca-alginate/Poly	408:423	arg1	PDA					481:483	Ca-alginate/PNIPAm@PDA	462:483	Ca-alginate/PNIPAm@PDA	462:483	This work reports a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere to control the agrochemicals release.
32479948	2	49	theme	Ca-alginate/Poly	408:423	arg1	microsphere					486:496	a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere	400:496	a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere to control the agrochemicals release	400:533	This work reports a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere to control the agrochemicals release.
32479948	3	50	with	Microsphere	536:546	arg1	network					577:583	a semi-interpenetrating network	553:583	a semi-interpenetrating network	553:583	Microsphere with a semi-interpenetrating network, which contained pH-sensitive Ca-alginate, temperature-sensitive poly(N-isopropylacrylamide) (PNIPAm), and sunlight-sensitive polydopamine (PDA), was characterized by thermogravimetric analysis, zeta potential, Fourier transform infrared spectroscopy, and scanning electron microscopy to prove the successful synthesis.
32479948	3	51	theme	zeta	780:783	arg1	potential					785:793	zeta potential	780:793	zeta potential	780:793	Microsphere with a semi-interpenetrating network, which contained pH-sensitive Ca-alginate, temperature-sensitive poly(N-isopropylacrylamide) (PNIPAm), and sunlight-sensitive polydopamine (PDA), was characterized by thermogravimetric analysis, zeta potential, Fourier transform infrared spectroscopy, and scanning electron microscopy to prove the successful synthesis.
32479948	5	52	theme	water	1169:1173	arg1	microsphere					1140:1150	the composite microsphere	1126:1150	the composite microsphere	1126:1150	The results indicated that the composite microsphere was a prosperous water and agrochemicals manager to effectively retain water and control the release of agrochemicals by external stimulation.
32479948	5	52	theme	water	1169:1173	arg1	manager					1193:1199	a prosperous water and agrochemicals manager	1156:1199	a prosperous water and agrochemicals manager	1156:1199	The results indicated that the composite microsphere was a prosperous water and agrochemicals manager to effectively retain water and control the release of agrochemicals by external stimulation.
32479948	1	53	theme	multi-responsive	187:202	arg1	system					223:228	The multi-responsive controlled-release system	183:228	The multi-responsive controlled-release system	183:228	The multi-responsive controlled-release system could enhance crop yield while improving utilization efficiency of agrochemicals, and minimize environmental pollution caused by agrochemicals overuse.
32479948	1	54	theme	agrochemicals	297:309	arg1	efficiency					283:292	utilization efficiency	271:292	utilization efficiency of agrochemicals	271:309	The multi-responsive controlled-release system could enhance crop yield while improving utilization efficiency of agrochemicals, and minimize environmental pollution caused by agrochemicals overuse.
32479948	0	55	theme	microspheres	92:103	arg1	characterization					14:29	characterization	14:29	characterization	14:29	Synthesis and characterization of dopamine-modified Ca-alginate/poly(N-isopropylacrylamide) microspheres for water retention and multi-responsive controlled release of agrochemicals.
32479948	0	55	theme	microspheres	92:103	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and characterization of dopamine-modified Ca-alginate/poly(N-isopropylacrylamide) microspheres for water retention and multi-responsive controlled release of agrochemicals.
32479948	0	55	theme	microspheres	92:103	arg1	release					157:163	multi-responsive controlled release	129:163	multi-responsive controlled release of agrochemicals	129:180	Synthesis and characterization of dopamine-modified Ca-alginate/poly(N-isopropylacrylamide) microspheres for water retention and multi-responsive controlled release of agrochemicals.
32479948	3	56	theme	temperature-sensitive	628:648	arg1	N-isopropylacrylamide					655:675	N-isopropylacrylamide	655:675	N-isopropylacrylamide	655:675	Microsphere with a semi-interpenetrating network, which contained pH-sensitive Ca-alginate, temperature-sensitive poly(N-isopropylacrylamide) (PNIPAm), and sunlight-sensitive polydopamine (PDA), was characterized by thermogravimetric analysis, zeta potential, Fourier transform infrared spectroscopy, and scanning electron microscopy to prove the successful synthesis.
32479948	3	56	theme	temperature-sensitive	628:648	arg1	PNIPAm					679:684	PNIPAm	679:684	PNIPAm	679:684	Microsphere with a semi-interpenetrating network, which contained pH-sensitive Ca-alginate, temperature-sensitive poly(N-isopropylacrylamide) (PNIPAm), and sunlight-sensitive polydopamine (PDA), was characterized by thermogravimetric analysis, zeta potential, Fourier transform infrared spectroscopy, and scanning electron microscopy to prove the successful synthesis.
32479948	3	56	theme	temperature-sensitive	628:648	arg1	poly					650:653	temperature-sensitive poly	628:653	temperature-sensitive poly(N-isopropylacrylamide) (PNIPAm)	628:685	Microsphere with a semi-interpenetrating network, which contained pH-sensitive Ca-alginate, temperature-sensitive poly(N-isopropylacrylamide) (PNIPAm), and sunlight-sensitive polydopamine (PDA), was characterized by thermogravimetric analysis, zeta potential, Fourier transform infrared spectroscopy, and scanning electron microscopy to prove the successful synthesis.
32479948	5	57	theme	prosperous	1158:1167	arg1	microsphere					1140:1150	the composite microsphere	1126:1150	the composite microsphere	1126:1150	The results indicated that the composite microsphere was a prosperous water and agrochemicals manager to effectively retain water and control the release of agrochemicals by external stimulation.
32479948	5	57	theme	prosperous	1158:1167	arg1	manager					1193:1199	a prosperous water and agrochemicals manager	1156:1199	a prosperous water and agrochemicals manager	1156:1199	The results indicated that the composite microsphere was a prosperous water and agrochemicals manager to effectively retain water and control the release of agrochemicals by external stimulation.
32479948	5	58	theme	agrochemicals	1179:1191	arg1	microsphere					1140:1150	the composite microsphere	1126:1150	the composite microsphere	1126:1150	The results indicated that the composite microsphere was a prosperous water and agrochemicals manager to effectively retain water and control the release of agrochemicals by external stimulation.
32479948	5	58	theme	agrochemicals	1179:1191	arg1	manager					1193:1199	a prosperous water and agrochemicals manager	1156:1199	a prosperous water and agrochemicals manager	1156:1199	The results indicated that the composite microsphere was a prosperous water and agrochemicals manager to effectively retain water and control the release of agrochemicals by external stimulation.
32479948	2	59	theme	polydopamine	448:459	arg1	PDA					481:483	Ca-alginate/PNIPAm@PDA	462:483	Ca-alginate/PNIPAm@PDA	462:483	This work reports a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere to control the agrochemicals release.
32479948	2	59	theme	polydopamine	448:459	arg1	microsphere					486:496	a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere	400:496	a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere to control the agrochemicals release	400:533	This work reports a novel Ca-alginate/Poly(N-isopropylacrylamide)@polydopamine (Ca-alginate/PNIPAm@PDA) microsphere to control the agrochemicals release.
32431500	12	0	theme	excellent	1530:1538	arg1	attachment					1546:1555	excellent cells attachment	1530:1555	excellent cells attachment	1530:1555	Furthermore, excellent cells attachment was observed on the CPHI scaffolds.
32431500	8	1	theme	Rat	1027:1029	arg1	cells					1055:1059	Rat marrow mesenchymal stem cells	1027:1059	Rat marrow mesenchymal stem cells from the wistar rat	1027:1079	Rat marrow mesenchymal stem cells from the wistar rat were cultured with the scaffolds.
32431500	11	2	theme	physical	1445:1452	arg1	properties					1467:1476	good physical and chemical properties	1440:1476	good physical and chemical properties	1440:1476	RESULTS The results reveal that the novel drug-loading core-shell scaffolds were successfully fabricated, which showed good physical and chemical properties and appropriate mechanical properties.
32431500	5	3	theme	shell	686:690	arg1	scaffolds					692:700	The shell scaffolds	682:700	The shell scaffolds	682:700	The shell scaffolds were electrospun using collagen, polycaprolactone and hydroxyapatite materials to form CPH composite scaffolds with the ones containing ICA microspheres named CPHI.
32431500	6	4	theme	core-shell	871:880	arg1	scaffolds					882:890	The core-shell scaffolds	867:890	The core-shell scaffolds	867:890	The core-shell scaffolds were then cross-linked by genipin.
32431500	3	5	theme	defects	533:539	arg1	repair					512:517	the repair	508:517	the repair of tibia bone defects in rabbit models	508:556	In this study, novel drug-loading core-shell scaffolds were fabricated via electrospinning and freeze drying to facilitate the repair of tibia bone defects in rabbit models.
32431500	1	6	theme	bone	268:271	arg1	regeneration					273:284	bone regeneration	268:284	bone regeneration	268:284	BACKGROUND Electrospinning is a widely used technology that can produce scaffolds with high porosity and surface area for bone regeneration.
32431500	8	7	theme	mesenchymal	1038:1048	arg1	cells					1055:1059	Rat marrow mesenchymal stem cells	1027:1059	Rat marrow mesenchymal stem cells from the wistar rat	1027:1079	Rat marrow mesenchymal stem cells from the wistar rat were cultured with the scaffolds.
32431500	11	8	theme	mechanical	1494:1503	arg1	properties					1505:1514	appropriate mechanical properties	1482:1514	appropriate mechanical properties	1482:1514	RESULTS The results reveal that the novel drug-loading core-shell scaffolds were successfully fabricated, which showed good physical and chemical properties and appropriate mechanical properties.
32431500	13	9	theme	new	1723:1725	arg1	bones					1727:1731	abundant new bones	1714:1731	abundant new bones	1714:1731	The results from radiography, micro-computed tomography, histological and immunohistochemical analysis demonstrated that abundant new bones were formed on the CPHI scaffolds.
32431500	10	10	theme	scaffolds	1261:1269	arg1	performance					1240:1250	the performance	1236:1250	the performance of these scaffolds in repairing the bone defects over 4 to 12 weeks	1236:1318	Adult rabbit models with tibial plateau defects were used to evaluate the performance of these scaffolds in repairing the bone defects over 4 to 12 weeks.
32431500	7	11	dep	morphology	932:941	arg1	The					928:930	The	928:930	The	928:930	The morphology, microstructure, physical and mechanical properties of the scaffolds were assessed.
32431500	13	12	from	radiography	1610:1620	arg1	results					1597:1603	The results	1593:1603	The results from radiography, micro-computed tomography, histological and immunohistochemical analysis	1593:1694	The results from radiography, micro-computed tomography, histological and immunohistochemical analysis demonstrated that abundant new bones were formed on the CPHI scaffolds.
32431500	4	13	theme	chitosan	659:666	arg1	microspheres					668:679	icariin (ICA)-loaded chitosan microspheres	638:679	icariin (ICA)-loaded chitosan microspheres	638:679	MATERIALS AND METHODS The collagen core scaffolds were freeze-dried containing icariin (ICA)-loaded chitosan microspheres.
32431500	0	14	theme	Bone	132:135	arg1	Defects					137:143	Rabbit Tibia Bone Defects	119:143	Rabbit Tibia Bone Defects	119:143	Electrospun Icariin-Loaded Core-Shell Collagen, Polycaprolactone, Hydroxyapatite Composite Scaffolds for the Repair of Rabbit Tibia Bone Defects.
32431500	2	15	theme	pore	306:309	arg1	sizes					311:315	the small pore sizes	296:315	the small pore sizes in electrospun scaffolds	296:340	However, the small pore sizes in electrospun scaffolds constrain cell growth and tissue-ingrowth.
32431500	10	16	with	models	1179:1184	arg1	defects					1206:1212	tibial plateau defects	1191:1212	tibial plateau defects	1191:1212	Adult rabbit models with tibial plateau defects were used to evaluate the performance of these scaffolds in repairing the bone defects over 4 to 12 weeks.
32431500	14	17	theme	engineering	1857:1867	arg1	applications					1869:1880	bone tissue engineering applications	1845:1880	bone tissue engineering applications	1845:1880	CONCLUSION These new core-shell composite scaffolds have great potential for bone tissue engineering applications and may lead to effective bone regeneration and repair.
32431500	14	18	theme	great	1825:1829	arg1	potential					1831:1839	great potential	1825:1839	great potential for bone tissue engineering applications	1825:1880	CONCLUSION These new core-shell composite scaffolds have great potential for bone tissue engineering applications and may lead to effective bone regeneration and repair.
32431500	1	19	theme	high	233:236	arg1	porosity					238:245	high porosity	233:245	high porosity	233:245	BACKGROUND Electrospinning is a widely used technology that can produce scaffolds with high porosity and surface area for bone regeneration.
32431500	14	20	theme	core-shell	1789:1798	arg1	scaffolds					1810:1818	These new core-shell composite scaffolds	1779:1818	These new core-shell composite scaffolds	1779:1818	CONCLUSION These new core-shell composite scaffolds have great potential for bone tissue engineering applications and may lead to effective bone regeneration and repair.
32431500	13	21	theme	abundant	1714:1721	arg1	bones					1727:1731	abundant new bones	1714:1731	abundant new bones	1714:1731	The results from radiography, micro-computed tomography, histological and immunohistochemical analysis demonstrated that abundant new bones were formed on the CPHI scaffolds.
32431500	1	22	theme	BACKGROUND	146:155	arg1	technology					190:199	a widely used technology	176:199	a widely used technology that can produce scaffolds with high porosity and surface area for bone regeneration	176:284	BACKGROUND Electrospinning is a widely used technology that can produce scaffolds with high porosity and surface area for bone regeneration.
32431500	1	22	theme	BACKGROUND	146:155	arg1	Electrospinning					157:171	BACKGROUND Electrospinning	146:171	BACKGROUND Electrospinning	146:171	BACKGROUND Electrospinning is a widely used technology that can produce scaffolds with high porosity and surface area for bone regeneration.
32431500	5	23	contain	containing	827:836	arg2	microspheres					842:853	ICA microspheres	838:853	ICA microspheres named CPHI	838:864	The shell scaffolds were electrospun using collagen, polycaprolactone and hydroxyapatite materials to form CPH composite scaffolds with the ones containing ICA microspheres named CPHI.
32431500	5	23	contain	containing	827:836	arg1	ones					822:825	the ones	818:825	the ones containing ICA microspheres named CPHI	818:864	The shell scaffolds were electrospun using collagen, polycaprolactone and hydroxyapatite materials to form CPH composite scaffolds with the ones containing ICA microspheres named CPHI.
32431500	10	24	theme	bone	1288:1291	arg1	defects					1293:1299	the bone defects	1284:1299	the bone defects	1284:1299	Adult rabbit models with tibial plateau defects were used to evaluate the performance of these scaffolds in repairing the bone defects over 4 to 12 weeks.
32431500	0	25	theme	Composite	81:89	arg1	Scaffolds					91:99	Electrospun Icariin-Loaded Core-Shell Collagen, Polycaprolactone, Hydroxyapatite Composite Scaffolds	0:99	Scaffolds	91:99	Electrospun Icariin-Loaded Core-Shell Collagen, Polycaprolactone, Hydroxyapatite Composite Scaffolds for the Repair of Rabbit Tibia Bone Defects.
32431500	13	26	theme	micro-computed	1623:1636	arg1	radiography					1610:1620	radiography	1610:1620	radiography	1610:1620	The results from radiography, micro-computed tomography, histological and immunohistochemical analysis demonstrated that abundant new bones were formed on the CPHI scaffolds.
32431500	13	26	theme	micro-computed	1623:1636	arg1	tomography					1638:1647	micro-computed tomography	1623:1647	micro-computed tomography	1623:1647	The results from radiography, micro-computed tomography, histological and immunohistochemical analysis demonstrated that abundant new bones were formed on the CPHI scaffolds.
32431500	2	27	from	sizes	311:315	arg1	scaffolds					332:340	electrospun scaffolds	320:340	electrospun scaffolds	320:340	However, the small pore sizes in electrospun scaffolds constrain cell growth and tissue-ingrowth.
32431500	10	28	dep	12	1311:1312	arg1	to					1308:1309	to	1308:1309	to	1308:1309	Adult rabbit models with tibial plateau defects were used to evaluate the performance of these scaffolds in repairing the bone defects over 4 to 12 weeks.
32431500	11	29	theme	novel	1357:1361	arg1	scaffolds					1387:1395	the novel drug-loading core-shell scaffolds	1353:1395	the novel drug-loading core-shell scaffolds	1353:1395	RESULTS The results reveal that the novel drug-loading core-shell scaffolds were successfully fabricated, which showed good physical and chemical properties and appropriate mechanical properties.
32431500	5	30	theme	composite	793:801	arg1	scaffolds					803:811	CPH composite scaffolds	789:811	CPH composite scaffolds	789:811	The shell scaffolds were electrospun using collagen, polycaprolactone and hydroxyapatite materials to form CPH composite scaffolds with the ones containing ICA microspheres named CPHI.
32431500	10	31	theme	Adult	1166:1170	arg1	models					1179:1184	Adult rabbit models	1166:1184	Adult rabbit models with tibial plateau defects	1166:1212	Adult rabbit models with tibial plateau defects were used to evaluate the performance of these scaffolds in repairing the bone defects over 4 to 12 weeks.
32431500	7	32	theme	scaffolds	1002:1010	arg1	microstructure					944:957	microstructure	944:957	microstructure	944:957	The morphology, microstructure, physical and mechanical properties of the scaffolds were assessed.
32431500	7	32	theme	scaffolds	1002:1010	arg1	morphology					932:941	morphology	932:941	morphology	932:941	The morphology, microstructure, physical and mechanical properties of the scaffolds were assessed.
32431500	7	32	theme	scaffolds	1002:1010	arg1	properties					984:993	mechanical properties	973:993	mechanical properties	973:993	The morphology, microstructure, physical and mechanical properties of the scaffolds were assessed.
32431500	11	33	dep	RESULTS	1321:1327	arg1	reveal					1341:1346	reveal	1341:1346	reveal that the novel drug-loading core-shell scaffolds were successfully fabricated, which showed good physical and chemical properties and appropriate mechanical properties	1341:1514	RESULTS The results reveal that the novel drug-loading core-shell scaffolds were successfully fabricated, which showed good physical and chemical properties and appropriate mechanical properties.
32431500	0	34	theme	Electrospun	0:10	arg1	Scaffolds					91:99	Electrospun Icariin-Loaded Core-Shell Collagen, Polycaprolactone, Hydroxyapatite Composite Scaffolds	0:99	Scaffolds	91:99	Electrospun Icariin-Loaded Core-Shell Collagen, Polycaprolactone, Hydroxyapatite Composite Scaffolds for the Repair of Rabbit Tibia Bone Defects.
32431500	0	35	theme	Core-Shell	27:36	arg1	Scaffolds					91:99	Electrospun Icariin-Loaded Core-Shell Collagen, Polycaprolactone, Hydroxyapatite Composite Scaffolds	0:99	Scaffolds	91:99	Electrospun Icariin-Loaded Core-Shell Collagen, Polycaprolactone, Hydroxyapatite Composite Scaffolds for the Repair of Rabbit Tibia Bone Defects.
32431500	10	36	theme	tibial	1191:1196	arg1	defects					1206:1212	tibial plateau defects	1191:1212	tibial plateau defects	1191:1212	Adult rabbit models with tibial plateau defects were used to evaluate the performance of these scaffolds in repairing the bone defects over 4 to 12 weeks.
32431500	4	37	theme	collagen	585:592	arg1	scaffolds					599:607	The collagen core scaffolds	581:607	MATERIALS AND METHODS The collagen core scaffolds	559:607	MATERIALS AND METHODS The collagen core scaffolds were freeze-dried containing icariin (ICA)-loaded chitosan microspheres.
32431500	10	38	used	used	1219:1222	arg2	models					1179:1184	Adult rabbit models	1166:1184	Adult rabbit models with tibial plateau defects	1166:1212	Adult rabbit models with tibial plateau defects were used to evaluate the performance of these scaffolds in repairing the bone defects over 4 to 12 weeks.
32431500	3	39	theme	freeze	480:485	arg1	drying					487:492	freeze drying	480:492	freeze drying	480:492	In this study, novel drug-loading core-shell scaffolds were fabricated via electrospinning and freeze drying to facilitate the repair of tibia bone defects in rabbit models.
32431500	5	40	theme	ICA	838:840	arg1	microspheres					842:853	ICA microspheres	838:853	ICA microspheres named CPHI	838:864	The shell scaffolds were electrospun using collagen, polycaprolactone and hydroxyapatite materials to form CPH composite scaffolds with the ones containing ICA microspheres named CPHI.
32431500	1	41	with	scaffolds	218:226	arg1	porosity					238:245	high porosity	233:245	high porosity	233:245	BACKGROUND Electrospinning is a widely used technology that can produce scaffolds with high porosity and surface area for bone regeneration.
32431500	1	41	with	scaffolds	218:226	arg1	area					259:262	surface area	251:262	surface area	251:262	BACKGROUND Electrospinning is a widely used technology that can produce scaffolds with high porosity and surface area for bone regeneration.
32431500	3	42	theme	drug-loading	406:417	arg1	scaffolds					430:438	novel drug-loading core-shell scaffolds	400:438	novel drug-loading core-shell scaffolds	400:438	In this study, novel drug-loading core-shell scaffolds were fabricated via electrospinning and freeze drying to facilitate the repair of tibia bone defects in rabbit models.
32431500	14	43	theme	bone	1908:1911	arg1	regeneration					1913:1924	effective bone regeneration	1898:1924	effective bone regeneration	1898:1924	CONCLUSION These new core-shell composite scaffolds have great potential for bone tissue engineering applications and may lead to effective bone regeneration and repair.
32431500	12	44	theme	CPHI	1577:1580	arg1	scaffolds					1582:1590	the CPHI scaffolds	1573:1590	the CPHI scaffolds	1573:1590	Furthermore, excellent cells attachment was observed on the CPHI scaffolds.
32431500	1	45	theme	surface	251:257	arg1	area					259:262	surface area	251:262	surface area	251:262	BACKGROUND Electrospinning is a widely used technology that can produce scaffolds with high porosity and surface area for bone regeneration.
32431500	12	46	theme	cells	1540:1544	arg1	attachment					1546:1555	excellent cells attachment	1530:1555	excellent cells attachment	1530:1555	Furthermore, excellent cells attachment was observed on the CPHI scaffolds.
32431500	8	47	theme	marrow	1031:1036	arg1	cells					1055:1059	Rat marrow mesenchymal stem cells	1027:1059	Rat marrow mesenchymal stem cells from the wistar rat	1027:1079	Rat marrow mesenchymal stem cells from the wistar rat were cultured with the scaffolds.
32431500	8	48	from	rat	1077:1079	arg1	cells					1055:1059	Rat marrow mesenchymal stem cells	1027:1059	Rat marrow mesenchymal stem cells from the wistar rat	1027:1079	Rat marrow mesenchymal stem cells from the wistar rat were cultured with the scaffolds.
32431500	11	49	theme	good	1440:1443	arg1	properties					1467:1476	good physical and chemical properties	1440:1476	good physical and chemical properties	1440:1476	RESULTS The results reveal that the novel drug-loading core-shell scaffolds were successfully fabricated, which showed good physical and chemical properties and appropriate mechanical properties.
32431500	3	50	theme	bone	528:531	arg1	defects					533:539	tibia bone defects	522:539	tibia bone defects in rabbit models	522:556	In this study, novel drug-loading core-shell scaffolds were fabricated via electrospinning and freeze drying to facilitate the repair of tibia bone defects in rabbit models.
32431500	8	51	theme	stem	1050:1053	arg1	cells					1055:1059	Rat marrow mesenchymal stem cells	1027:1059	Rat marrow mesenchymal stem cells from the wistar rat	1027:1079	Rat marrow mesenchymal stem cells from the wistar rat were cultured with the scaffolds.
32431500	11	52	theme	appropriate	1482:1492	arg1	properties					1505:1514	appropriate mechanical properties	1482:1514	appropriate mechanical properties	1482:1514	RESULTS The results reveal that the novel drug-loading core-shell scaffolds were successfully fabricated, which showed good physical and chemical properties and appropriate mechanical properties.
32431500	3	53	from	defects	533:539	arg1	models					551:556	rabbit models	544:556	rabbit models	544:556	In this study, novel drug-loading core-shell scaffolds were fabricated via electrospinning and freeze drying to facilitate the repair of tibia bone defects in rabbit models.
32431500	2	54	theme	small	300:304	arg1	sizes					311:315	the small pore sizes	296:315	the small pore sizes in electrospun scaffolds	296:340	However, the small pore sizes in electrospun scaffolds constrain cell growth and tissue-ingrowth.
32431500	14	55	theme	tissue	1850:1855	arg1	applications					1869:1880	bone tissue engineering applications	1845:1880	bone tissue engineering applications	1845:1880	CONCLUSION These new core-shell composite scaffolds have great potential for bone tissue engineering applications and may lead to effective bone regeneration and repair.
32431500	13	56	theme	immunohistochemical	1667:1685	arg1	analysis					1687:1694	histological and immunohistochemical analysis	1650:1694	histological and immunohistochemical analysis	1650:1694	The results from radiography, micro-computed tomography, histological and immunohistochemical analysis demonstrated that abundant new bones were formed on the CPHI scaffolds.
32431500	13	56	theme	immunohistochemical	1667:1685	arg1	radiography					1610:1620	radiography	1610:1620	radiography	1610:1620	The results from radiography, micro-computed tomography, histological and immunohistochemical analysis demonstrated that abundant new bones were formed on the CPHI scaffolds.
32431500	1	57	theme	used	185:188	arg1	technology					190:199	a widely used technology	176:199	a widely used technology that can produce scaffolds with high porosity and surface area for bone regeneration	176:284	BACKGROUND Electrospinning is a widely used technology that can produce scaffolds with high porosity and surface area for bone regeneration.
32431500	1	57	theme	used	185:188	arg1	Electrospinning					157:171	BACKGROUND Electrospinning	146:171	BACKGROUND Electrospinning	146:171	BACKGROUND Electrospinning is a widely used technology that can produce scaffolds with high porosity and surface area for bone regeneration.
32431500	8	58	theme	wistar	1070:1075	arg1	rat					1077:1079	the wistar rat	1066:1079	the wistar rat	1066:1079	Rat marrow mesenchymal stem cells from the wistar rat were cultured with the scaffolds.
32431500	14	59	dep	CONCLUSION	1768:1777	arg1	lead					1890:1893	lead	1890:1893	may lead to effective bone regeneration and repair	1886:1935	CONCLUSION These new core-shell composite scaffolds have great potential for bone tissue engineering applications and may lead to effective bone regeneration and repair.
32431500	14	59	dep	CONCLUSION	1768:1777	arg1	have					1820:1823	have	1820:1823	have great potential for bone tissue engineering applications	1820:1880	CONCLUSION These new core-shell composite scaffolds have great potential for bone tissue engineering applications and may lead to effective bone regeneration and repair.
32431500	3	60	from	repair	512:517	arg1	models					551:556	rabbit models	544:556	rabbit models	544:556	In this study, novel drug-loading core-shell scaffolds were fabricated via electrospinning and freeze drying to facilitate the repair of tibia bone defects in rabbit models.
32431500	2	61	theme	electrospun	320:330	arg1	scaffolds					332:340	electrospun scaffolds	320:340	electrospun scaffolds	320:340	However, the small pore sizes in electrospun scaffolds constrain cell growth and tissue-ingrowth.
32431500	11	62	theme	core-shell	1376:1385	arg1	scaffolds					1387:1395	the novel drug-loading core-shell scaffolds	1353:1395	the novel drug-loading core-shell scaffolds	1353:1395	RESULTS The results reveal that the novel drug-loading core-shell scaffolds were successfully fabricated, which showed good physical and chemical properties and appropriate mechanical properties.
32431500	0	63	theme	Defects	137:143	arg1	Repair					109:114	the Repair	105:114	the Repair of Rabbit Tibia Bone Defects	105:143	Electrospun Icariin-Loaded Core-Shell Collagen, Polycaprolactone, Hydroxyapatite Composite Scaffolds for the Repair of Rabbit Tibia Bone Defects.
32431500	4	64	theme	-loaded	651:657	arg1	microspheres					668:679	icariin (ICA)-loaded chitosan microspheres	638:679	icariin (ICA)-loaded chitosan microspheres	638:679	MATERIALS AND METHODS The collagen core scaffolds were freeze-dried containing icariin (ICA)-loaded chitosan microspheres.
32431500	5	65	theme	hydroxyapatite	756:769	arg1	materials					771:779	collagen, polycaprolactone and hydroxyapatite materials	725:779	collagen, polycaprolactone and hydroxyapatite materials	725:779	The shell scaffolds were electrospun using collagen, polycaprolactone and hydroxyapatite materials to form CPH composite scaffolds with the ones containing ICA microspheres named CPHI.
32431500	14	66	contain	have	1820:1823	arg1	scaffolds					1810:1818	These new core-shell composite scaffolds	1779:1818	These new core-shell composite scaffolds	1779:1818	CONCLUSION These new core-shell composite scaffolds have great potential for bone tissue engineering applications and may lead to effective bone regeneration and repair.
32431500	14	66	contain	have	1820:1823	arg2	potential					1831:1839	great potential	1825:1839	great potential for bone tissue engineering applications	1825:1880	CONCLUSION These new core-shell composite scaffolds have great potential for bone tissue engineering applications and may lead to effective bone regeneration and repair.
32431500	13	67	theme	histological	1650:1661	arg1	analysis					1687:1694	histological and immunohistochemical analysis	1650:1694	histological and immunohistochemical analysis	1650:1694	The results from radiography, micro-computed tomography, histological and immunohistochemical analysis demonstrated that abundant new bones were formed on the CPHI scaffolds.
32431500	13	67	theme	histological	1650:1661	arg1	radiography					1610:1620	radiography	1610:1620	radiography	1610:1620	The results from radiography, micro-computed tomography, histological and immunohistochemical analysis demonstrated that abundant new bones were formed on the CPHI scaffolds.
32431500	5	68	theme	polycaprolactone	735:750	arg1	materials					771:779	collagen, polycaprolactone and hydroxyapatite materials	725:779	collagen, polycaprolactone and hydroxyapatite materials	725:779	The shell scaffolds were electrospun using collagen, polycaprolactone and hydroxyapatite materials to form CPH composite scaffolds with the ones containing ICA microspheres named CPHI.
32431500	5	69	theme	collagen	725:732	arg1	materials					771:779	collagen, polycaprolactone and hydroxyapatite materials	725:779	collagen, polycaprolactone and hydroxyapatite materials	725:779	The shell scaffolds were electrospun using collagen, polycaprolactone and hydroxyapatite materials to form CPH composite scaffolds with the ones containing ICA microspheres named CPHI.
32431500	14	70	theme	composite	1800:1808	arg1	scaffolds					1810:1818	These new core-shell composite scaffolds	1779:1818	These new core-shell composite scaffolds	1779:1818	CONCLUSION These new core-shell composite scaffolds have great potential for bone tissue engineering applications and may lead to effective bone regeneration and repair.
32431500	14	71	theme	new	1785:1787	arg1	scaffolds					1810:1818	These new core-shell composite scaffolds	1779:1818	These new core-shell composite scaffolds	1779:1818	CONCLUSION These new core-shell composite scaffolds have great potential for bone tissue engineering applications and may lead to effective bone regeneration and repair.
32431500	3	72	from	models	551:556	arg1	repair					512:517	the repair	508:517	the repair of tibia bone defects in rabbit models	508:556	In this study, novel drug-loading core-shell scaffolds were fabricated via electrospinning and freeze drying to facilitate the repair of tibia bone defects in rabbit models.
32431500	4	73	dep	MATERIALS	559:567	arg1	scaffolds					599:607	The collagen core scaffolds	581:607	MATERIALS AND METHODS The collagen core scaffolds	559:607	MATERIALS AND METHODS The collagen core scaffolds were freeze-dried containing icariin (ICA)-loaded chitosan microspheres.
32431500	9	74	theme	cell	1119:1122	arg1	adhesion					1124:1131	cell adhesion	1119:1131	cell adhesion	1119:1131	The cell adhesion and proliferation were analysed.
32431500	0	75	theme	Tibia	126:130	arg1	Defects					137:143	Rabbit Tibia Bone Defects	119:143	Rabbit Tibia Bone Defects	119:143	Electrospun Icariin-Loaded Core-Shell Collagen, Polycaprolactone, Hydroxyapatite Composite Scaffolds for the Repair of Rabbit Tibia Bone Defects.
32431500	10	76	theme	rabbit	1172:1177	arg1	models					1179:1184	Adult rabbit models	1166:1184	Adult rabbit models with tibial plateau defects	1166:1212	Adult rabbit models with tibial plateau defects were used to evaluate the performance of these scaffolds in repairing the bone defects over 4 to 12 weeks.
32431500	11	77	theme	drug-loading	1363:1374	arg1	scaffolds					1387:1395	the novel drug-loading core-shell scaffolds	1353:1395	the novel drug-loading core-shell scaffolds	1353:1395	RESULTS The results reveal that the novel drug-loading core-shell scaffolds were successfully fabricated, which showed good physical and chemical properties and appropriate mechanical properties.
32431500	5	78	theme	CPH	789:791	arg1	scaffolds					803:811	CPH composite scaffolds	789:811	CPH composite scaffolds	789:811	The shell scaffolds were electrospun using collagen, polycaprolactone and hydroxyapatite materials to form CPH composite scaffolds with the ones containing ICA microspheres named CPHI.
32431500	0	79	theme	Icariin-Loaded	12:25	arg1	Scaffolds					91:99	Electrospun Icariin-Loaded Core-Shell Collagen, Polycaprolactone, Hydroxyapatite Composite Scaffolds	0:99	Scaffolds	91:99	Electrospun Icariin-Loaded Core-Shell Collagen, Polycaprolactone, Hydroxyapatite Composite Scaffolds for the Repair of Rabbit Tibia Bone Defects.
32431500	3	80	theme	novel	400:404	arg1	scaffolds					430:438	novel drug-loading core-shell scaffolds	400:438	novel drug-loading core-shell scaffolds	400:438	In this study, novel drug-loading core-shell scaffolds were fabricated via electrospinning and freeze drying to facilitate the repair of tibia bone defects in rabbit models.
32431500	0	81	theme	Collagen	38:45	arg1	Scaffolds					91:99	Electrospun Icariin-Loaded Core-Shell Collagen, Polycaprolactone, Hydroxyapatite Composite Scaffolds	0:99	Scaffolds	91:99	Electrospun Icariin-Loaded Core-Shell Collagen, Polycaprolactone, Hydroxyapatite Composite Scaffolds for the Repair of Rabbit Tibia Bone Defects.
32431500	10	82	theme	plateau	1198:1204	arg1	defects					1206:1212	tibial plateau defects	1191:1212	tibial plateau defects	1191:1212	Adult rabbit models with tibial plateau defects were used to evaluate the performance of these scaffolds in repairing the bone defects over 4 to 12 weeks.
32431500	0	83	dep	Scaffolds	91:99	arg1	Polycaprolactone					48:63	Electrospun Icariin-Loaded Core-Shell Collagen, Polycaprolactone, Hydroxyapatite Composite Scaffolds	0:99	Polycaprolactone	48:63	Electrospun Icariin-Loaded Core-Shell Collagen, Polycaprolactone, Hydroxyapatite Composite Scaffolds for the Repair of Rabbit Tibia Bone Defects.
32431500	4	84	theme	core	594:597	arg1	scaffolds					599:607	The collagen core scaffolds	581:607	MATERIALS AND METHODS The collagen core scaffolds	559:607	MATERIALS AND METHODS The collagen core scaffolds were freeze-dried containing icariin (ICA)-loaded chitosan microspheres.
32431500	2	85	dep	cell	352:355	arg1	tissue-ingrowth					368:382	tissue-ingrowth	368:382	tissue-ingrowth	368:382	However, the small pore sizes in electrospun scaffolds constrain cell growth and tissue-ingrowth.
32431500	2	85	dep	cell	352:355	arg1	growth					357:362	growth	357:362	growth	357:362	However, the small pore sizes in electrospun scaffolds constrain cell growth and tissue-ingrowth.
32431500	14	86	theme	bone	1845:1848	arg1	applications					1869:1880	bone tissue engineering applications	1845:1880	bone tissue engineering applications	1845:1880	CONCLUSION These new core-shell composite scaffolds have great potential for bone tissue engineering applications and may lead to effective bone regeneration and repair.
32431500	0	87	theme	Rabbit	119:124	arg1	Defects					137:143	Rabbit Tibia Bone Defects	119:143	Rabbit Tibia Bone Defects	119:143	Electrospun Icariin-Loaded Core-Shell Collagen, Polycaprolactone, Hydroxyapatite Composite Scaffolds for the Repair of Rabbit Tibia Bone Defects.
32431500	0	88	theme	Hydroxyapatite	66:79	arg1	Scaffolds					91:99	Electrospun Icariin-Loaded Core-Shell Collagen, Polycaprolactone, Hydroxyapatite Composite Scaffolds	0:99	Scaffolds	91:99	Electrospun Icariin-Loaded Core-Shell Collagen, Polycaprolactone, Hydroxyapatite Composite Scaffolds for the Repair of Rabbit Tibia Bone Defects.
32431500	3	89	theme	core-shell	419:428	arg1	scaffolds					430:438	novel drug-loading core-shell scaffolds	400:438	novel drug-loading core-shell scaffolds	400:438	In this study, novel drug-loading core-shell scaffolds were fabricated via electrospinning and freeze drying to facilitate the repair of tibia bone defects in rabbit models.
32431500	3	90	theme	tibia	522:526	arg1	defects					533:539	tibia bone defects	522:539	tibia bone defects in rabbit models	522:556	In this study, novel drug-loading core-shell scaffolds were fabricated via electrospinning and freeze drying to facilitate the repair of tibia bone defects in rabbit models.
32431500	7	91	theme	mechanical	973:982	arg1	properties					984:993	mechanical properties	973:993	mechanical properties	973:993	The morphology, microstructure, physical and mechanical properties of the scaffolds were assessed.
32431500	14	92	theme	effective	1898:1906	arg1	regeneration					1913:1924	effective bone regeneration	1898:1924	effective bone regeneration	1898:1924	CONCLUSION These new core-shell composite scaffolds have great potential for bone tissue engineering applications and may lead to effective bone regeneration and repair.
32431500	13	93	theme	CPHI	1752:1755	arg1	scaffolds					1757:1765	the CPHI scaffolds	1748:1765	the CPHI scaffolds	1748:1765	The results from radiography, micro-computed tomography, histological and immunohistochemical analysis demonstrated that abundant new bones were formed on the CPHI scaffolds.
32431500	11	94	theme	chemical	1458:1465	arg1	properties					1467:1476	good physical and chemical properties	1440:1476	good physical and chemical properties	1440:1476	RESULTS The results reveal that the novel drug-loading core-shell scaffolds were successfully fabricated, which showed good physical and chemical properties and appropriate mechanical properties.
32431500	3	95	theme	rabbit	544:549	arg1	models					551:556	rabbit models	544:556	rabbit models	544:556	In this study, novel drug-loading core-shell scaffolds were fabricated via electrospinning and freeze drying to facilitate the repair of tibia bone defects in rabbit models.
32431500	12	96	located	observed	1561:1568	arg2	attachment					1546:1555	excellent cells attachment	1530:1555	excellent cells attachment	1530:1555	Furthermore, excellent cells attachment was observed on the CPHI scaffolds.
32431500	12	96	located	observed	1561:1568	arg1	scaffolds					1582:1590	the CPHI scaffolds	1573:1590	the CPHI scaffolds	1573:1590	Furthermore, excellent cells attachment was observed on the CPHI scaffolds.
34724014	1	0	from	efficacy	178:185	arg1	CRC					218:220	CRC	218:220	CRC	218:220	The aim of this study was to investigate the efficacy of kefir on colorectal cancer (CRC) via regulating the microbiota structure in the colon using the azoxymethane/dextran sulfate sodium (AOM/DSS) induced CRC mouse model.
34724014	1	0	from	efficacy	178:185	arg1	cancer					210:215	colorectal cancer	199:215	colorectal cancer (CRC)	199:221	The aim of this study was to investigate the efficacy of kefir on colorectal cancer (CRC) via regulating the microbiota structure in the colon using the azoxymethane/dextran sulfate sodium (AOM/DSS) induced CRC mouse model.
34724014	3	1	theme	transcribed	488:498	arg1	spacer					500:505	internally transcribed spacer 2	477:507	internally transcribed spacer 2 (ITS2)	477:514	The gut microbiota composition was assessed by internally transcribed spacer 2 (ITS2) and 16S rRNA high-throughput sequencing.
34724014	3	1	theme	transcribed	488:498	arg1	ITS2					510:513	ITS2	510:513	ITS2	510:513	The gut microbiota composition was assessed by internally transcribed spacer 2 (ITS2) and 16S rRNA high-throughput sequencing.
34724014	1	2	theme	induced	332:338	arg1	model					350:354	the azoxymethane/dextran sulfate sodium (AOM/DSS) induced CRC mouse model	282:354	the azoxymethane/dextran sulfate sodium (AOM/DSS) induced CRC mouse model	282:354	The aim of this study was to investigate the efficacy of kefir on colorectal cancer (CRC) via regulating the microbiota structure in the colon using the azoxymethane/dextran sulfate sodium (AOM/DSS) induced CRC mouse model.
34724014	5	3	theme	immunity	765:772	arg1	IL-17a					803:808	IL-17a	803:808	IL-17a	803:808	The results indicated that the size and the amount of tumor were decreased and the immunity regulators (TNF-α, IL-6, and IL-17a) and oncocyte proliferation indicator (Ki67, NF-κB, and β-catenin) were all decreased.
34724014	5	3	theme	immunity	765:772	arg1	TNF-α					786:790	TNF-α	786:790	TNF-α	786:790	The results indicated that the size and the amount of tumor were decreased and the immunity regulators (TNF-α, IL-6, and IL-17a) and oncocyte proliferation indicator (Ki67, NF-κB, and β-catenin) were all decreased.
34724014	5	3	theme	immunity	765:772	arg1	regulators					774:783	the immunity regulators	761:783	the immunity regulators (TNF-α, IL-6, and IL-17a)	761:809	The results indicated that the size and the amount of tumor were decreased and the immunity regulators (TNF-α, IL-6, and IL-17a) and oncocyte proliferation indicator (Ki67, NF-κB, and β-catenin) were all decreased.
34724014	5	3	theme	immunity	765:772	arg1	IL-6					793:796	IL-6	793:796	IL-6	793:796	The results indicated that the size and the amount of tumor were decreased and the immunity regulators (TNF-α, IL-6, and IL-17a) and oncocyte proliferation indicator (Ki67, NF-κB, and β-catenin) were all decreased.
34724014	1	4	theme	microbiota	242:251	arg1	structure					253:261	the microbiota structure	238:261	the microbiota structure in the colon using the azoxymethane/dextran sulfate sodium (AOM/DSS) induced CRC mouse model	238:354	The aim of this study was to investigate the efficacy of kefir on colorectal cancer (CRC) via regulating the microbiota structure in the colon using the azoxymethane/dextran sulfate sodium (AOM/DSS) induced CRC mouse model.
34724014	0	5	theme	gut	117:119	arg1	microbiota					121:130	the gut microbiota	113:130	the gut microbiota	113:130	Supplementation of kefir ameliorates azoxymethane/dextran sulfate sodium induced colorectal cancer by modulating the gut microbiota.
34724014	1	6	theme	CRC	340:342	arg1	model					350:354	the azoxymethane/dextran sulfate sodium (AOM/DSS) induced CRC mouse model	282:354	the azoxymethane/dextran sulfate sodium (AOM/DSS) induced CRC mouse model	282:354	The aim of this study was to investigate the efficacy of kefir on colorectal cancer (CRC) via regulating the microbiota structure in the colon using the azoxymethane/dextran sulfate sodium (AOM/DSS) induced CRC mouse model.
34724014	1	7	theme	mouse	344:348	arg1	model					350:354	the azoxymethane/dextran sulfate sodium (AOM/DSS) induced CRC mouse model	282:354	the azoxymethane/dextran sulfate sodium (AOM/DSS) induced CRC mouse model	282:354	The aim of this study was to investigate the efficacy of kefir on colorectal cancer (CRC) via regulating the microbiota structure in the colon using the azoxymethane/dextran sulfate sodium (AOM/DSS) induced CRC mouse model.
34724014	7	8	theme	Ascomycota/Basidiomycota	1049:1072	arg1	Firmicutes/Bacteroidetes					1014:1037	The Firmicutes/Bacteroidetes ratio and Ascomycota/Basidiomycota ratio	1010:1078	The Firmicutes/Bacteroidetes ratio and Ascomycota/Basidiomycota ratio	1010:1078	The Firmicutes/Bacteroidetes ratio and Ascomycota/Basidiomycota ratio were decreased at the phylum level; the relative abundance of probiotics was increased and the pathogenic bacterium (Clostridium sensu stricto, Aspergillus and Talaromyces) were decreased after supplementation of kefir.
34724014	7	8	theme	Ascomycota/Basidiomycota	1049:1072	arg1	ratio					1074:1078	Ascomycota/Basidiomycota ratio	1049:1078	Ascomycota/Basidiomycota ratio	1049:1078	The Firmicutes/Bacteroidetes ratio and Ascomycota/Basidiomycota ratio were decreased at the phylum level; the relative abundance of probiotics was increased and the pathogenic bacterium (Clostridium sensu stricto, Aspergillus and Talaromyces) were decreased after supplementation of kefir.
34724014	3	9	theme	16S	520:522	arg1	sequencing					545:554	16S rRNA high-throughput sequencing	520:554	16S rRNA high-throughput sequencing	520:554	The gut microbiota composition was assessed by internally transcribed spacer 2 (ITS2) and 16S rRNA high-throughput sequencing.
34724014	4	10	theme	cell	636:639	arg1	regulators					655:664	cell proliferation regulators	636:664	cell proliferation regulators	636:664	Furthermore, the biomarkers associated with the gut barrier, inflammation, and cell proliferation regulators were evaluated.
34724014	5	11	dep	regulators	774:783	arg1	IL-17a					803:808	IL-17a	803:808	IL-17a	803:808	The results indicated that the size and the amount of tumor were decreased and the immunity regulators (TNF-α, IL-6, and IL-17a) and oncocyte proliferation indicator (Ki67, NF-κB, and β-catenin) were all decreased.
34724014	5	11	dep	regulators	774:783	arg1	TNF-α					786:790	TNF-α	786:790	TNF-α	786:790	The results indicated that the size and the amount of tumor were decreased and the immunity regulators (TNF-α, IL-6, and IL-17a) and oncocyte proliferation indicator (Ki67, NF-κB, and β-catenin) were all decreased.
34724014	5	11	dep	regulators	774:783	arg1	regulators					774:783	the immunity regulators	761:783	the immunity regulators (TNF-α, IL-6, and IL-17a)	761:809	The results indicated that the size and the amount of tumor were decreased and the immunity regulators (TNF-α, IL-6, and IL-17a) and oncocyte proliferation indicator (Ki67, NF-κB, and β-catenin) were all decreased.
34724014	5	11	dep	regulators	774:783	arg1	IL-6					793:796	IL-6	793:796	IL-6	793:796	The results indicated that the size and the amount of tumor were decreased and the immunity regulators (TNF-α, IL-6, and IL-17a) and oncocyte proliferation indicator (Ki67, NF-κB, and β-catenin) were all decreased.
34724014	7	12	dep	Firmicutes/Bacteroidetes	1014:1037	arg1	Firmicutes/Bacteroidetes					1014:1037	The Firmicutes/Bacteroidetes ratio and Ascomycota/Basidiomycota ratio	1010:1078	The Firmicutes/Bacteroidetes ratio and Ascomycota/Basidiomycota ratio	1010:1078	The Firmicutes/Bacteroidetes ratio and Ascomycota/Basidiomycota ratio were decreased at the phylum level; the relative abundance of probiotics was increased and the pathogenic bacterium (Clostridium sensu stricto, Aspergillus and Talaromyces) were decreased after supplementation of kefir.
34724014	7	12	dep	Firmicutes/Bacteroidetes	1014:1037	arg1	ratio					1039:1043	ratio	1039:1043	ratio	1039:1043	The Firmicutes/Bacteroidetes ratio and Ascomycota/Basidiomycota ratio were decreased at the phylum level; the relative abundance of probiotics was increased and the pathogenic bacterium (Clostridium sensu stricto, Aspergillus and Talaromyces) were decreased after supplementation of kefir.
34724014	7	12	dep	Firmicutes/Bacteroidetes	1014:1037	arg1	ratio					1074:1078	Ascomycota/Basidiomycota ratio	1049:1078	Ascomycota/Basidiomycota ratio	1049:1078	The Firmicutes/Bacteroidetes ratio and Ascomycota/Basidiomycota ratio were decreased at the phylum level; the relative abundance of probiotics was increased and the pathogenic bacterium (Clostridium sensu stricto, Aspergillus and Talaromyces) were decreased after supplementation of kefir.
34724014	2	13	theme	treatment	369:377	arg1	group					379:383	the treatment group	365:383	the treatment group	365:383	Mice in the treatment group were orally administered with milk or kefir.
34724014	7	14	dep	bacterium	1186:1194	arg1	Talaromyces					1240:1250	Talaromyces	1240:1250	Talaromyces	1240:1250	The Firmicutes/Bacteroidetes ratio and Ascomycota/Basidiomycota ratio were decreased at the phylum level; the relative abundance of probiotics was increased and the pathogenic bacterium (Clostridium sensu stricto, Aspergillus and Talaromyces) were decreased after supplementation of kefir.
34724014	7	14	dep	bacterium	1186:1194	arg1	Aspergillus					1224:1234	Aspergillus	1224:1234	Aspergillus	1224:1234	The Firmicutes/Bacteroidetes ratio and Ascomycota/Basidiomycota ratio were decreased at the phylum level; the relative abundance of probiotics was increased and the pathogenic bacterium (Clostridium sensu stricto, Aspergillus and Talaromyces) were decreased after supplementation of kefir.
34724014	7	14	dep	bacterium	1186:1194	arg1	stricto					1215:1221	Clostridium sensu stricto	1197:1221	Clostridium sensu stricto	1197:1221	The Firmicutes/Bacteroidetes ratio and Ascomycota/Basidiomycota ratio were decreased at the phylum level; the relative abundance of probiotics was increased and the pathogenic bacterium (Clostridium sensu stricto, Aspergillus and Talaromyces) were decreased after supplementation of kefir.
34724014	5	15	theme	proliferation	824:836	arg1	indicator					838:846	oncocyte proliferation indicator	815:846	oncocyte proliferation indicator (Ki67, NF-κB, and β-catenin)	815:875	The results indicated that the size and the amount of tumor were decreased and the immunity regulators (TNF-α, IL-6, and IL-17a) and oncocyte proliferation indicator (Ki67, NF-κB, and β-catenin) were all decreased.
34724014	7	16	theme	relative	1120:1127	arg1	abundance					1129:1137	the relative abundance	1116:1137	the relative abundance of probiotics	1116:1151	The Firmicutes/Bacteroidetes ratio and Ascomycota/Basidiomycota ratio were decreased at the phylum level; the relative abundance of probiotics was increased and the pathogenic bacterium (Clostridium sensu stricto, Aspergillus and Talaromyces) were decreased after supplementation of kefir.
34724014	6	17	theme	fatty	919:923	arg1	SCFAs					932:936	SCFAs	932:936	SCFAs	932:936	Increased short chain fatty acids (SCFAs) lowered the pH in the colon and helped enhance the intestinal barrier.
34724014	6	17	theme	fatty	919:923	arg1	acids					925:929	Increased short chain fatty acids	897:929	Increased short chain fatty acids (SCFAs)	897:937	Increased short chain fatty acids (SCFAs) lowered the pH in the colon and helped enhance the intestinal barrier.
34724014	2	18	from	Mice	357:360	arg1	group					379:383	the treatment group	365:383	the treatment group	365:383	Mice in the treatment group were orally administered with milk or kefir.
34724014	1	19	theme	kefir	190:194	arg1	efficacy					178:185	the efficacy	174:185	the efficacy of kefir on colorectal cancer (CRC)	174:221	The aim of this study was to investigate the efficacy of kefir on colorectal cancer (CRC) via regulating the microbiota structure in the colon using the azoxymethane/dextran sulfate sodium (AOM/DSS) induced CRC mouse model.
34724014	6	20	theme	chain	913:917	arg1	SCFAs					932:936	SCFAs	932:936	SCFAs	932:936	Increased short chain fatty acids (SCFAs) lowered the pH in the colon and helped enhance the intestinal barrier.
34724014	6	20	theme	chain	913:917	arg1	acids					925:929	Increased short chain fatty acids	897:929	Increased short chain fatty acids (SCFAs)	897:937	Increased short chain fatty acids (SCFAs) lowered the pH in the colon and helped enhance the intestinal barrier.
34724014	1	21	from	structure	253:261	arg1	colon					270:274	the colon	266:274	the colon using the azoxymethane/dextran sulfate sodium (AOM/DSS) induced CRC mouse model	266:354	The aim of this study was to investigate the efficacy of kefir on colorectal cancer (CRC) via regulating the microbiota structure in the colon using the azoxymethane/dextran sulfate sodium (AOM/DSS) induced CRC mouse model.
34724014	3	22	theme	rRNA	524:527	arg1	sequencing					545:554	16S rRNA high-throughput sequencing	520:554	16S rRNA high-throughput sequencing	520:554	The gut microbiota composition was assessed by internally transcribed spacer 2 (ITS2) and 16S rRNA high-throughput sequencing.
34724014	6	23	theme	intestinal	990:999	arg1	barrier					1001:1007	the intestinal barrier	986:1007	the intestinal barrier	986:1007	Increased short chain fatty acids (SCFAs) lowered the pH in the colon and helped enhance the intestinal barrier.
34724014	3	24	theme	microbiota	438:447	arg1	composition					449:459	The gut microbiota composition	430:459	The gut microbiota composition	430:459	The gut microbiota composition was assessed by internally transcribed spacer 2 (ITS2) and 16S rRNA high-throughput sequencing.
34724014	0	25	theme	kefir	19:23	arg1	Supplementation					0:14	Supplementation	0:14	Supplementation of kefir	0:23	Supplementation of kefir ameliorates azoxymethane/dextran sulfate sodium induced colorectal cancer by modulating the gut microbiota.
34724014	6	26	theme	short	907:911	arg1	SCFAs					932:936	SCFAs	932:936	SCFAs	932:936	Increased short chain fatty acids (SCFAs) lowered the pH in the colon and helped enhance the intestinal barrier.
34724014	6	26	theme	short	907:911	arg1	acids					925:929	Increased short chain fatty acids	897:929	Increased short chain fatty acids (SCFAs)	897:937	Increased short chain fatty acids (SCFAs) lowered the pH in the colon and helped enhance the intestinal barrier.
34724014	3	27	theme	high-throughput	529:543	arg1	sequencing					545:554	16S rRNA high-throughput sequencing	520:554	16S rRNA high-throughput sequencing	520:554	The gut microbiota composition was assessed by internally transcribed spacer 2 (ITS2) and 16S rRNA high-throughput sequencing.
34724014	1	28	theme	colorectal	199:208	arg1	CRC					218:220	CRC	218:220	CRC	218:220	The aim of this study was to investigate the efficacy of kefir on colorectal cancer (CRC) via regulating the microbiota structure in the colon using the azoxymethane/dextran sulfate sodium (AOM/DSS) induced CRC mouse model.
34724014	1	28	theme	colorectal	199:208	arg1	cancer					210:215	colorectal cancer	199:215	colorectal cancer (CRC)	199:221	The aim of this study was to investigate the efficacy of kefir on colorectal cancer (CRC) via regulating the microbiota structure in the colon using the azoxymethane/dextran sulfate sodium (AOM/DSS) induced CRC mouse model.
34724014	0	29	theme	sulfate	58:64	arg1	sodium					66:71	azoxymethane/dextran sulfate sodium	37:71	azoxymethane/dextran sulfate sodium induced colorectal cancer	37:97	Supplementation of kefir ameliorates azoxymethane/dextran sulfate sodium induced colorectal cancer by modulating the gut microbiota.
34724014	6	30	theme	Increased	897:905	arg1	SCFAs					932:936	SCFAs	932:936	SCFAs	932:936	Increased short chain fatty acids (SCFAs) lowered the pH in the colon and helped enhance the intestinal barrier.
34724014	6	30	theme	Increased	897:905	arg1	acids					925:929	Increased short chain fatty acids	897:929	Increased short chain fatty acids (SCFAs)	897:937	Increased short chain fatty acids (SCFAs) lowered the pH in the colon and helped enhance the intestinal barrier.
34724014	7	31	theme	Clostridium	1197:1207	arg1	Talaromyces					1240:1250	Talaromyces	1240:1250	Talaromyces	1240:1250	The Firmicutes/Bacteroidetes ratio and Ascomycota/Basidiomycota ratio were decreased at the phylum level; the relative abundance of probiotics was increased and the pathogenic bacterium (Clostridium sensu stricto, Aspergillus and Talaromyces) were decreased after supplementation of kefir.
34724014	7	31	theme	Clostridium	1197:1207	arg1	Aspergillus					1224:1234	Aspergillus	1224:1234	Aspergillus	1224:1234	The Firmicutes/Bacteroidetes ratio and Ascomycota/Basidiomycota ratio were decreased at the phylum level; the relative abundance of probiotics was increased and the pathogenic bacterium (Clostridium sensu stricto, Aspergillus and Talaromyces) were decreased after supplementation of kefir.
34724014	7	31	theme	Clostridium	1197:1207	arg1	stricto					1215:1221	Clostridium sensu stricto	1197:1221	Clostridium sensu stricto	1197:1221	The Firmicutes/Bacteroidetes ratio and Ascomycota/Basidiomycota ratio were decreased at the phylum level; the relative abundance of probiotics was increased and the pathogenic bacterium (Clostridium sensu stricto, Aspergillus and Talaromyces) were decreased after supplementation of kefir.
34724014	4	32	theme	proliferation	641:653	arg1	regulators					655:664	cell proliferation regulators	636:664	cell proliferation regulators	636:664	Furthermore, the biomarkers associated with the gut barrier, inflammation, and cell proliferation regulators were evaluated.
34724014	0	33	theme	azoxymethane/dextran	37:56	arg1	sodium					66:71	azoxymethane/dextran sulfate sodium	37:71	azoxymethane/dextran sulfate sodium induced colorectal cancer	37:97	Supplementation of kefir ameliorates azoxymethane/dextran sulfate sodium induced colorectal cancer by modulating the gut microbiota.
34724014	7	34	theme	phylum	1102:1107	arg1	level					1109:1113	the phylum level	1098:1113	the phylum level	1098:1113	The Firmicutes/Bacteroidetes ratio and Ascomycota/Basidiomycota ratio were decreased at the phylum level; the relative abundance of probiotics was increased and the pathogenic bacterium (Clostridium sensu stricto, Aspergillus and Talaromyces) were decreased after supplementation of kefir.
34724014	5	35	dep	indicator	838:846	arg1	NF-κB					855:859	NF-κB	855:859	NF-κB	855:859	The results indicated that the size and the amount of tumor were decreased and the immunity regulators (TNF-α, IL-6, and IL-17a) and oncocyte proliferation indicator (Ki67, NF-κB, and β-catenin) were all decreased.
34724014	5	35	dep	indicator	838:846	arg1	β-catenin					866:874	β-catenin	866:874	β-catenin	866:874	The results indicated that the size and the amount of tumor were decreased and the immunity regulators (TNF-α, IL-6, and IL-17a) and oncocyte proliferation indicator (Ki67, NF-κB, and β-catenin) were all decreased.
34724014	5	35	dep	indicator	838:846	arg1	Ki67					849:852	Ki67	849:852	Ki67	849:852	The results indicated that the size and the amount of tumor were decreased and the immunity regulators (TNF-α, IL-6, and IL-17a) and oncocyte proliferation indicator (Ki67, NF-κB, and β-catenin) were all decreased.
34724014	3	36	theme	gut	434:436	arg1	composition					449:459	The gut microbiota composition	430:459	The gut microbiota composition	430:459	The gut microbiota composition was assessed by internally transcribed spacer 2 (ITS2) and 16S rRNA high-throughput sequencing.
34724014	0	37	theme	induced	73:79	arg1	cancer					92:97	azoxymethane/dextran sulfate sodium induced colorectal cancer	37:97	azoxymethane/dextran sulfate sodium induced colorectal cancer	37:97	Supplementation of kefir ameliorates azoxymethane/dextran sulfate sodium induced colorectal cancer by modulating the gut microbiota.
34724014	7	38	theme	probiotics	1142:1151	arg1	abundance					1129:1137	the relative abundance	1116:1137	the relative abundance of probiotics	1116:1151	The Firmicutes/Bacteroidetes ratio and Ascomycota/Basidiomycota ratio were decreased at the phylum level; the relative abundance of probiotics was increased and the pathogenic bacterium (Clostridium sensu stricto, Aspergillus and Talaromyces) were decreased after supplementation of kefir.
34724014	5	39	theme	tumor	736:740	arg1	tumor					736:740	tumor	736:740	tumor	736:740	The results indicated that the size and the amount of tumor were decreased and the immunity regulators (TNF-α, IL-6, and IL-17a) and oncocyte proliferation indicator (Ki67, NF-κB, and β-catenin) were all decreased.
34724014	5	39	theme	tumor	736:740	arg1	size					713:716	the size	709:716	the size	709:716	The results indicated that the size and the amount of tumor were decreased and the immunity regulators (TNF-α, IL-6, and IL-17a) and oncocyte proliferation indicator (Ki67, NF-κB, and β-catenin) were all decreased.
34724014	5	39	theme	tumor	736:740	arg1	amount					726:731	the amount	722:731	the amount of tumor	722:740	The results indicated that the size and the amount of tumor were decreased and the immunity regulators (TNF-α, IL-6, and IL-17a) and oncocyte proliferation indicator (Ki67, NF-κB, and β-catenin) were all decreased.
34724014	0	40	theme	sodium	66:71	arg1	cancer					92:97	azoxymethane/dextran sulfate sodium induced colorectal cancer	37:97	azoxymethane/dextran sulfate sodium induced colorectal cancer	37:97	Supplementation of kefir ameliorates azoxymethane/dextran sulfate sodium induced colorectal cancer by modulating the gut microbiota.
34724014	7	41	theme	kefir	1293:1297	arg1	supplementation					1274:1288	supplementation	1274:1288	supplementation of kefir	1274:1297	The Firmicutes/Bacteroidetes ratio and Ascomycota/Basidiomycota ratio were decreased at the phylum level; the relative abundance of probiotics was increased and the pathogenic bacterium (Clostridium sensu stricto, Aspergillus and Talaromyces) were decreased after supplementation of kefir.
34724014	8	42	theme	induced	1389:1395	arg1	cancer					1408:1413	AOM/DSS induced colorectal cancer	1381:1413	AOM/DSS induced colorectal cancer	1381:1413	Consequently, kefir could regulate the gut microbiota composition and ameliorate AOM/DSS induced colorectal cancer.
34724014	8	43	theme	microbiota	1343:1352	arg1	composition					1354:1364	the gut microbiota composition	1335:1364	the gut microbiota composition	1335:1364	Consequently, kefir could regulate the gut microbiota composition and ameliorate AOM/DSS induced colorectal cancer.
34724014	8	44	theme	gut	1339:1341	arg1	composition					1354:1364	the gut microbiota composition	1335:1364	the gut microbiota composition	1335:1364	Consequently, kefir could regulate the gut microbiota composition and ameliorate AOM/DSS induced colorectal cancer.
34724014	8	45	theme	AOM/DSS	1381:1387	arg1	cancer					1408:1413	AOM/DSS induced colorectal cancer	1381:1413	AOM/DSS induced colorectal cancer	1381:1413	Consequently, kefir could regulate the gut microbiota composition and ameliorate AOM/DSS induced colorectal cancer.
34724014	7	46	theme	pathogenic	1175:1184	arg1	bacterium					1186:1194	the pathogenic bacterium	1171:1194	the pathogenic bacterium (Clostridium sensu stricto, Aspergillus and Talaromyces)	1171:1251	The Firmicutes/Bacteroidetes ratio and Ascomycota/Basidiomycota ratio were decreased at the phylum level; the relative abundance of probiotics was increased and the pathogenic bacterium (Clostridium sensu stricto, Aspergillus and Talaromyces) were decreased after supplementation of kefir.
34724014	1	47	theme	study	149:153	arg1	aim					137:139	The aim	133:139	The aim of this study	133:153	The aim of this study was to investigate the efficacy of kefir on colorectal cancer (CRC) via regulating the microbiota structure in the colon using the azoxymethane/dextran sulfate sodium (AOM/DSS) induced CRC mouse model.
34724014	5	48	theme	oncocyte	815:822	arg1	indicator					838:846	oncocyte proliferation indicator	815:846	oncocyte proliferation indicator (Ki67, NF-κB, and β-catenin)	815:875	The results indicated that the size and the amount of tumor were decreased and the immunity regulators (TNF-α, IL-6, and IL-17a) and oncocyte proliferation indicator (Ki67, NF-κB, and β-catenin) were all decreased.
34724014	4	49	theme	gut	605:607	arg1	barrier					609:615	the gut barrier	601:615	the gut barrier	601:615	Furthermore, the biomarkers associated with the gut barrier, inflammation, and cell proliferation regulators were evaluated.
34724014	0	50	theme	colorectal	81:90	arg1	cancer					92:97	azoxymethane/dextran sulfate sodium induced colorectal cancer	37:97	azoxymethane/dextran sulfate sodium induced colorectal cancer	37:97	Supplementation of kefir ameliorates azoxymethane/dextran sulfate sodium induced colorectal cancer by modulating the gut microbiota.
34724014	8	51	theme	colorectal	1397:1406	arg1	cancer					1408:1413	AOM/DSS induced colorectal cancer	1381:1413	AOM/DSS induced colorectal cancer	1381:1413	Consequently, kefir could regulate the gut microbiota composition and ameliorate AOM/DSS induced colorectal cancer.
34724014	7	52	theme	sensu	1209:1213	arg1	Talaromyces					1240:1250	Talaromyces	1240:1250	Talaromyces	1240:1250	The Firmicutes/Bacteroidetes ratio and Ascomycota/Basidiomycota ratio were decreased at the phylum level; the relative abundance of probiotics was increased and the pathogenic bacterium (Clostridium sensu stricto, Aspergillus and Talaromyces) were decreased after supplementation of kefir.
34724014	7	52	theme	sensu	1209:1213	arg1	Aspergillus					1224:1234	Aspergillus	1224:1234	Aspergillus	1224:1234	The Firmicutes/Bacteroidetes ratio and Ascomycota/Basidiomycota ratio were decreased at the phylum level; the relative abundance of probiotics was increased and the pathogenic bacterium (Clostridium sensu stricto, Aspergillus and Talaromyces) were decreased after supplementation of kefir.
34724014	7	52	theme	sensu	1209:1213	arg1	stricto					1215:1221	Clostridium sensu stricto	1197:1221	Clostridium sensu stricto	1197:1221	The Firmicutes/Bacteroidetes ratio and Ascomycota/Basidiomycota ratio were decreased at the phylum level; the relative abundance of probiotics was increased and the pathogenic bacterium (Clostridium sensu stricto, Aspergillus and Talaromyces) were decreased after supplementation of kefir.
31765286	0	0	theme	Bacterial	81:89	arg1	solanacearum					115:126	the Bacterial Wilt Pathogen Ralstonia solanacearum	77:126	the Bacterial Wilt Pathogen Ralstonia solanacearum	77:126	Trehalose Synthesis Contributes to Osmotic Stress Tolerance and Virulence of the Bacterial Wilt Pathogen Ralstonia solanacearum.
31765286	6	1	theme	plant	995:999	arg1	vessels					1007:1013	plant xylem vessels	995:1013	plant xylem vessels	995:1013	This trehalose-nonproducing mutant had reduced tolerance to osmotic stress, which the bacterium likely experiences in plant xylem vessels.
31765286	8	2	theme	trehalose	1321:1329	arg1	biosynthesis					1331:1342	trehalose biosynthesis	1321:1342	trehalose biosynthesis	1321:1342	Further, the wild-type strain out-competed the trehalose-nonproducing mutant by over 600-fold when tomato plants were coinoculated with both strains, showing that trehalose biosynthesis helps R. solanacearum overcome environmental stresses during infection.
31765286	9	3	theme	dominant	1579:1586	arg1	pathway					1608:1614	the dominant trehalose synthesis pathway	1575:1614	the dominant trehalose synthesis pathway in R. solanacearum	1575:1633	An otsA (trehalose-6-phosphate synthase) single mutant behaved similarly to ΔtreY/treS/otsA in all experimental settings, suggesting that the OtsAB pathway is the dominant trehalose synthesis pathway in R. solanacearum.
31765286	9	3	theme	dominant	1579:1586	arg1	pathway					1564:1570	the OtsAB pathway	1554:1570	the OtsAB pathway	1554:1570	An otsA (trehalose-6-phosphate synthase) single mutant behaved similarly to ΔtreY/treS/otsA in all experimental settings, suggesting that the OtsAB pathway is the dominant trehalose synthesis pathway in R. solanacearum.
31765286	9	4	theme	single	1457:1462	arg1	mutant					1464:1469	An otsA (trehalose-6-phosphate synthase) single mutant	1416:1469	An otsA (trehalose-6-phosphate synthase) single mutant	1416:1469	An otsA (trehalose-6-phosphate synthase) single mutant behaved similarly to ΔtreY/treS/otsA in all experimental settings, suggesting that the OtsAB pathway is the dominant trehalose synthesis pathway in R. solanacearum.
31765286	0	5	theme	Pathogen	96:103	arg1	solanacearum					115:126	the Bacterial Wilt Pathogen Ralstonia solanacearum	77:126	the Bacterial Wilt Pathogen Ralstonia solanacearum	77:126	Trehalose Synthesis Contributes to Osmotic Stress Tolerance and Virulence of the Bacterial Wilt Pathogen Ralstonia solanacearum.
31765286	4	6	dep	source	521:526	arg1	the					517:519	the	517:519	the	517:519	To investigate the source and role of trehalose metabolism during wilt disease, we evaluated the effects of deleting the three trehalose synthesis pathways in the pathogen: TreYZ, TreS, and OtsAB, as well as its sole trehalase, TreA.
31765286	9	7	theme	trehalose	1588:1596	arg1	pathway					1608:1614	the dominant trehalose synthesis pathway	1575:1614	the dominant trehalose synthesis pathway in R. solanacearum	1575:1633	An otsA (trehalose-6-phosphate synthase) single mutant behaved similarly to ΔtreY/treS/otsA in all experimental settings, suggesting that the OtsAB pathway is the dominant trehalose synthesis pathway in R. solanacearum.
31765286	9	7	theme	trehalose	1588:1596	arg1	pathway					1564:1570	the OtsAB pathway	1554:1570	the OtsAB pathway	1554:1570	An otsA (trehalose-6-phosphate synthase) single mutant behaved similarly to ΔtreY/treS/otsA in all experimental settings, suggesting that the OtsAB pathway is the dominant trehalose synthesis pathway in R. solanacearum.
31765286	0	8	theme	Wilt	91:94	arg1	solanacearum					115:126	the Bacterial Wilt Pathogen Ralstonia solanacearum	77:126	the Bacterial Wilt Pathogen Ralstonia solanacearum	77:126	Trehalose Synthesis Contributes to Osmotic Stress Tolerance and Virulence of the Bacterial Wilt Pathogen Ralstonia solanacearum.
31765286	9	9	theme	OtsAB	1558:1562	arg1	pathway					1608:1614	the dominant trehalose synthesis pathway	1575:1614	the dominant trehalose synthesis pathway in R. solanacearum	1575:1633	An otsA (trehalose-6-phosphate synthase) single mutant behaved similarly to ΔtreY/treS/otsA in all experimental settings, suggesting that the OtsAB pathway is the dominant trehalose synthesis pathway in R. solanacearum.
31765286	9	9	theme	OtsAB	1558:1562	arg1	pathway					1564:1570	the OtsAB pathway	1554:1570	the OtsAB pathway	1554:1570	An otsA (trehalose-6-phosphate synthase) single mutant behaved similarly to ΔtreY/treS/otsA in all experimental settings, suggesting that the OtsAB pathway is the dominant trehalose synthesis pathway in R. solanacearum.
31765286	4	10	theme	sole	714:717	arg1	trehalase					719:727	its sole trehalase	710:727	its sole trehalase	710:727	To investigate the source and role of trehalose metabolism during wilt disease, we evaluated the effects of deleting the three trehalose synthesis pathways in the pathogen: TreYZ, TreS, and OtsAB, as well as its sole trehalase, TreA.
31765286	4	10	theme	sole	714:717	arg1	TreA					730:733	TreA	730:733	TreA	730:733	To investigate the source and role of trehalose metabolism during wilt disease, we evaluated the effects of deleting the three trehalose synthesis pathways in the pathogen: TreYZ, TreS, and OtsAB, as well as its sole trehalase, TreA.
31765286	7	11	theme	R.	1141:1142	arg1	solanacearum					1144:1155	wild-type R. solanacearum	1131:1155	wild-type R. solanacearum	1131:1155	Following naturalistic soil-soak inoculation of tomato plants, this triple mutant did not cause disease as well as wild-type R. solanacearum.
31765286	2	12	theme	stress	310:315	arg1	tolerance					317:325	stress tolerance	310:325	stress tolerance across all kingdoms of life	310:353	The disaccharide trehalose, implicated in stress tolerance across all kingdoms of life, is enriched in sap from R. solanacearum-infected tomato plants.
31765286	2	13	theme	life	350:353	arg1	kingdoms					338:345	all kingdoms	334:345	all kingdoms of life	334:353	The disaccharide trehalose, implicated in stress tolerance across all kingdoms of life, is enriched in sap from R. solanacearum-infected tomato plants.
31765286	0	14	theme	solanacearum	115:126	arg1	Virulence					64:72	Virulence	64:72	Virulence	64:72	Trehalose Synthesis Contributes to Osmotic Stress Tolerance and Virulence of the Bacterial Wilt Pathogen Ralstonia solanacearum.
31765286	0	14	theme	solanacearum	115:126	arg1	Tolerance					50:58	Osmotic Stress Tolerance	35:58	Osmotic Stress Tolerance	35:58	Trehalose Synthesis Contributes to Osmotic Stress Tolerance and Virulence of the Bacterial Wilt Pathogen Ralstonia solanacearum.
31765286	6	15	from	experiences	980:990	arg1	vessels					1007:1013	plant xylem vessels	995:1013	plant xylem vessels	995:1013	This trehalose-nonproducing mutant had reduced tolerance to osmotic stress, which the bacterium likely experiences in plant xylem vessels.
31765286	9	16	theme	experimental	1515:1526	arg1	settings					1528:1535	all experimental settings	1511:1535	all experimental settings	1511:1535	An otsA (trehalose-6-phosphate synthase) single mutant behaved similarly to ΔtreY/treS/otsA in all experimental settings, suggesting that the OtsAB pathway is the dominant trehalose synthesis pathway in R. solanacearum.
31765286	6	17	contain	had	912:914	arg1	mutant					905:910	This trehalose-nonproducing mutant	877:910	This trehalose-nonproducing mutant	877:910	This trehalose-nonproducing mutant had reduced tolerance to osmotic stress, which the bacterium likely experiences in plant xylem vessels.
31765286	6	17	contain	had	912:914	arg2	tolerance					924:932	reduced tolerance	916:932	reduced tolerance to osmotic stress, which the bacterium likely experiences in plant xylem vessels	916:1013	This trehalose-nonproducing mutant had reduced tolerance to osmotic stress, which the bacterium likely experiences in plant xylem vessels.
31765286	5	18	theme	wild-type	830:838	arg1	strain					840:845	the wild-type strain	826:845	the wild-type strain missing the trehalase enzyme	826:874	A quadruple treY/treS/otsA/treA mutant produced 30-fold less intracellular trehalose than the wild-type strain missing the trehalase enzyme.
31765286	0	19	theme	Ralstonia	105:113	arg1	solanacearum					115:126	the Bacterial Wilt Pathogen Ralstonia solanacearum	77:126	the Bacterial Wilt Pathogen Ralstonia solanacearum	77:126	Trehalose Synthesis Contributes to Osmotic Stress Tolerance and Virulence of the Bacterial Wilt Pathogen Ralstonia solanacearum.
31765286	3	20	theme	xylem	433:437	arg1	sap					439:441	xylem sap	433:441	xylem sap	433:441	Trehalose in xylem sap could be synthesized by the bacterium, the plant, or both.
31765286	8	21	theme	environmental	1375:1387	arg1	stresses					1389:1396	environmental stresses	1375:1396	environmental stresses	1375:1396	Further, the wild-type strain out-competed the trehalose-nonproducing mutant by over 600-fold when tomato plants were coinoculated with both strains, showing that trehalose biosynthesis helps R. solanacearum overcome environmental stresses during infection.
31765286	1	22	theme	chemical	198:205	arg1	composition					207:217	the chemical composition	194:217	the chemical composition of host xylem sap during bacterial wilt disease	194:265	The xylem-dwelling plant pathogen Ralstonia solanacearum changes the chemical composition of host xylem sap during bacterial wilt disease.
31765286	7	23	theme	triple	1084:1089	arg1	mutant					1091:1096	this triple mutant	1079:1096	this triple mutant	1079:1096	Following naturalistic soil-soak inoculation of tomato plants, this triple mutant did not cause disease as well as wild-type R. solanacearum.
31765286	9	24	theme	R.	1619:1620	arg1	solanacearum					1622:1633	R. solanacearum	1619:1633	R. solanacearum	1619:1633	An otsA (trehalose-6-phosphate synthase) single mutant behaved similarly to ΔtreY/treS/otsA in all experimental settings, suggesting that the OtsAB pathway is the dominant trehalose synthesis pathway in R. solanacearum.
31765286	4	25	from	pathways	649:656	arg1	pathogen					665:672	the pathogen	661:672	the pathogen	661:672	To investigate the source and role of trehalose metabolism during wilt disease, we evaluated the effects of deleting the three trehalose synthesis pathways in the pathogen: TreYZ, TreS, and OtsAB, as well as its sole trehalase, TreA.
31765286	2	26	theme	tomato	405:410	arg1	plants					412:417	R. solanacearum-infected tomato plants	380:417	R. solanacearum-infected tomato plants	380:417	The disaccharide trehalose, implicated in stress tolerance across all kingdoms of life, is enriched in sap from R. solanacearum-infected tomato plants.
31765286	0	27	theme	Trehalose	0:8	arg1	Synthesis					10:18	Trehalose Synthesis	0:18	Trehalose Synthesis	0:18	Trehalose Synthesis Contributes to Osmotic Stress Tolerance and Virulence of the Bacterial Wilt Pathogen Ralstonia solanacearum.
31765286	4	28	theme	metabolism	550:559	arg1	role					532:535	role	532:535	role	532:535	To investigate the source and role of trehalose metabolism during wilt disease, we evaluated the effects of deleting the three trehalose synthesis pathways in the pathogen: TreYZ, TreS, and OtsAB, as well as its sole trehalase, TreA.
31765286	4	28	theme	metabolism	550:559	arg1	source					521:526	source	521:526	source	521:526	To investigate the source and role of trehalose metabolism during wilt disease, we evaluated the effects of deleting the three trehalose synthesis pathways in the pathogen: TreYZ, TreS, and OtsAB, as well as its sole trehalase, TreA.
31765286	2	29	theme	solanacearum-infected	383:403	arg1	plants					412:417	R. solanacearum-infected tomato plants	380:417	R. solanacearum-infected tomato plants	380:417	The disaccharide trehalose, implicated in stress tolerance across all kingdoms of life, is enriched in sap from R. solanacearum-infected tomato plants.
31765286	4	30	theme	trehalose	540:548	arg1	metabolism					550:559	trehalose metabolism	540:559	trehalose metabolism during wilt disease	540:579	To investigate the source and role of trehalose metabolism during wilt disease, we evaluated the effects of deleting the three trehalose synthesis pathways in the pathogen: TreYZ, TreS, and OtsAB, as well as its sole trehalase, TreA.
31765286	1	31	theme	host	222:225	arg1	sap					233:235	host xylem sap	222:235	host xylem sap	222:235	The xylem-dwelling plant pathogen Ralstonia solanacearum changes the chemical composition of host xylem sap during bacterial wilt disease.
31765286	5	32	theme	less	792:795	arg1	trehalose					811:819	30-fold less intracellular trehalose	784:819	30-fold less intracellular trehalose	784:819	A quadruple treY/treS/otsA/treA mutant produced 30-fold less intracellular trehalose than the wild-type strain missing the trehalase enzyme.
31765286	4	33	theme	wilt	568:571	arg1	disease					573:579	wilt disease	568:579	wilt disease	568:579	To investigate the source and role of trehalose metabolism during wilt disease, we evaluated the effects of deleting the three trehalose synthesis pathways in the pathogen: TreYZ, TreS, and OtsAB, as well as its sole trehalase, TreA.
31765286	1	34	theme	xylem	227:231	arg1	sap					233:235	host xylem sap	222:235	host xylem sap	222:235	The xylem-dwelling plant pathogen Ralstonia solanacearum changes the chemical composition of host xylem sap during bacterial wilt disease.
31765286	0	35	theme	Stress	43:48	arg1	Tolerance					50:58	Osmotic Stress Tolerance	35:58	Osmotic Stress Tolerance	35:58	Trehalose Synthesis Contributes to Osmotic Stress Tolerance and Virulence of the Bacterial Wilt Pathogen Ralstonia solanacearum.
31765286	7	36	theme	tomato	1064:1069	arg1	plants					1071:1076	tomato plants	1064:1076	tomato plants	1064:1076	Following naturalistic soil-soak inoculation of tomato plants, this triple mutant did not cause disease as well as wild-type R. solanacearum.
31765286	4	37	theme	synthesis	639:647	arg1	OtsAB					692:696	OtsAB	692:696	OtsAB	692:696	To investigate the source and role of trehalose metabolism during wilt disease, we evaluated the effects of deleting the three trehalose synthesis pathways in the pathogen: TreYZ, TreS, and OtsAB, as well as its sole trehalase, TreA.
31765286	4	37	theme	synthesis	639:647	arg1	TreYZ					675:679	TreYZ	675:679	TreYZ	675:679	To investigate the source and role of trehalose metabolism during wilt disease, we evaluated the effects of deleting the three trehalose synthesis pathways in the pathogen: TreYZ, TreS, and OtsAB, as well as its sole trehalase, TreA.
31765286	4	37	theme	synthesis	639:647	arg1	pathways					649:656	the three trehalose synthesis pathways	619:656	the three trehalose synthesis pathways in the pathogen: TreYZ, TreS, and OtsAB	619:696	To investigate the source and role of trehalose metabolism during wilt disease, we evaluated the effects of deleting the three trehalose synthesis pathways in the pathogen: TreYZ, TreS, and OtsAB, as well as its sole trehalase, TreA.
31765286	8	38	dep	R.	1350:1351	arg1	solanacearum					1353:1364	R. solanacearum	1350:1364	R. solanacearum	1350:1364	Further, the wild-type strain out-competed the trehalose-nonproducing mutant by over 600-fold when tomato plants were coinoculated with both strains, showing that trehalose biosynthesis helps R. solanacearum overcome environmental stresses during infection.
31765286	8	39	theme	tomato	1257:1262	arg1	plants					1264:1269	tomato plants	1257:1269	tomato plants	1257:1269	Further, the wild-type strain out-competed the trehalose-nonproducing mutant by over 600-fold when tomato plants were coinoculated with both strains, showing that trehalose biosynthesis helps R. solanacearum overcome environmental stresses during infection.
31765286	1	40	theme	sap	233:235	arg1	composition					207:217	the chemical composition	194:217	the chemical composition of host xylem sap during bacterial wilt disease	194:265	The xylem-dwelling plant pathogen Ralstonia solanacearum changes the chemical composition of host xylem sap during bacterial wilt disease.
31765286	0	41	theme	Osmotic	35:41	arg1	Tolerance					50:58	Osmotic Stress Tolerance	35:58	Osmotic Stress Tolerance	35:58	Trehalose Synthesis Contributes to Osmotic Stress Tolerance and Virulence of the Bacterial Wilt Pathogen Ralstonia solanacearum.
31765286	7	42	theme	plants	1071:1076	arg1	inoculation					1049:1059	naturalistic soil-soak inoculation	1026:1059	naturalistic soil-soak inoculation of tomato plants	1026:1076	Following naturalistic soil-soak inoculation of tomato plants, this triple mutant did not cause disease as well as wild-type R. solanacearum.
31765286	9	43	theme	trehalose-6-phosphate	1425:1445	arg1	otsA					1419:1422	otsA	1419:1422	An otsA (trehalose-6-phosphate synthase) single mutant	1416:1469	An otsA (trehalose-6-phosphate synthase) single mutant behaved similarly to ΔtreY/treS/otsA in all experimental settings, suggesting that the OtsAB pathway is the dominant trehalose synthesis pathway in R. solanacearum.
31765286	9	43	theme	trehalose-6-phosphate	1425:1445	arg1	synthase					1447:1454	trehalose-6-phosphate synthase	1425:1454	trehalose-6-phosphate synthase	1425:1454	An otsA (trehalose-6-phosphate synthase) single mutant behaved similarly to ΔtreY/treS/otsA in all experimental settings, suggesting that the OtsAB pathway is the dominant trehalose synthesis pathway in R. solanacearum.
31765286	4	44	theme	trehalose	629:637	arg1	OtsAB					692:696	OtsAB	692:696	OtsAB	692:696	To investigate the source and role of trehalose metabolism during wilt disease, we evaluated the effects of deleting the three trehalose synthesis pathways in the pathogen: TreYZ, TreS, and OtsAB, as well as its sole trehalase, TreA.
31765286	4	44	theme	trehalose	629:637	arg1	TreYZ					675:679	TreYZ	675:679	TreYZ	675:679	To investigate the source and role of trehalose metabolism during wilt disease, we evaluated the effects of deleting the three trehalose synthesis pathways in the pathogen: TreYZ, TreS, and OtsAB, as well as its sole trehalase, TreA.
31765286	4	44	theme	trehalose	629:637	arg1	pathways					649:656	the three trehalose synthesis pathways	619:656	the three trehalose synthesis pathways in the pathogen: TreYZ, TreS, and OtsAB	619:696	To investigate the source and role of trehalose metabolism during wilt disease, we evaluated the effects of deleting the three trehalose synthesis pathways in the pathogen: TreYZ, TreS, and OtsAB, as well as its sole trehalase, TreA.
31765286	5	45	theme	quadruple	738:746	arg1	mutant					768:773	A quadruple treY/treS/otsA/treA mutant	736:773	A quadruple treY/treS/otsA/treA mutant	736:773	A quadruple treY/treS/otsA/treA mutant produced 30-fold less intracellular trehalose than the wild-type strain missing the trehalase enzyme.
31765286	5	46	theme	trehalase	859:867	arg1	enzyme					869:874	the trehalase enzyme	855:874	the trehalase enzyme	855:874	A quadruple treY/treS/otsA/treA mutant produced 30-fold less intracellular trehalose than the wild-type strain missing the trehalase enzyme.
31765286	8	47	theme	trehalose-nonproducing	1205:1226	arg1	mutant					1228:1233	the trehalose-nonproducing mutant	1201:1233	the trehalose-nonproducing mutant	1201:1233	Further, the wild-type strain out-competed the trehalose-nonproducing mutant by over 600-fold when tomato plants were coinoculated with both strains, showing that trehalose biosynthesis helps R. solanacearum overcome environmental stresses during infection.
31765286	5	48	theme	treY/treS/otsA/treA	748:766	arg1	mutant					768:773	A quadruple treY/treS/otsA/treA mutant	736:773	A quadruple treY/treS/otsA/treA mutant	736:773	A quadruple treY/treS/otsA/treA mutant produced 30-fold less intracellular trehalose than the wild-type strain missing the trehalase enzyme.
31765286	6	49	theme	reduced	916:922	arg1	tolerance					924:932	reduced tolerance	916:932	reduced tolerance to osmotic stress, which the bacterium likely experiences in plant xylem vessels	916:1013	This trehalose-nonproducing mutant had reduced tolerance to osmotic stress, which the bacterium likely experiences in plant xylem vessels.
31765286	9	50	theme	synthesis	1598:1606	arg1	pathway					1608:1614	the dominant trehalose synthesis pathway	1575:1614	the dominant trehalose synthesis pathway in R. solanacearum	1575:1633	An otsA (trehalose-6-phosphate synthase) single mutant behaved similarly to ΔtreY/treS/otsA in all experimental settings, suggesting that the OtsAB pathway is the dominant trehalose synthesis pathway in R. solanacearum.
31765286	9	50	theme	synthesis	1598:1606	arg1	pathway					1564:1570	the OtsAB pathway	1554:1570	the OtsAB pathway	1554:1570	An otsA (trehalose-6-phosphate synthase) single mutant behaved similarly to ΔtreY/treS/otsA in all experimental settings, suggesting that the OtsAB pathway is the dominant trehalose synthesis pathway in R. solanacearum.
31765286	9	51	from	pathway	1608:1614	arg1	solanacearum					1622:1633	R. solanacearum	1619:1633	R. solanacearum	1619:1633	An otsA (trehalose-6-phosphate synthase) single mutant behaved similarly to ΔtreY/treS/otsA in all experimental settings, suggesting that the OtsAB pathway is the dominant trehalose synthesis pathway in R. solanacearum.
31765286	7	52	theme	naturalistic	1026:1037	arg1	inoculation					1049:1059	naturalistic soil-soak inoculation	1026:1059	naturalistic soil-soak inoculation of tomato plants	1026:1076	Following naturalistic soil-soak inoculation of tomato plants, this triple mutant did not cause disease as well as wild-type R. solanacearum.
31765286	1	53	theme	pathogen	154:161	arg1	solanacearum					173:184	The xylem-dwelling plant pathogen Ralstonia solanacearum	129:184	The xylem-dwelling plant pathogen Ralstonia solanacearum	129:184	The xylem-dwelling plant pathogen Ralstonia solanacearum changes the chemical composition of host xylem sap during bacterial wilt disease.
31765286	7	54	theme	wild-type	1131:1139	arg1	solanacearum					1144:1155	wild-type R. solanacearum	1131:1155	wild-type R. solanacearum	1131:1155	Following naturalistic soil-soak inoculation of tomato plants, this triple mutant did not cause disease as well as wild-type R. solanacearum.
31765286	2	55	from	plants	412:417	arg1	sap					371:373	sap	371:373	sap from R. solanacearum-infected tomato plants	371:417	The disaccharide trehalose, implicated in stress tolerance across all kingdoms of life, is enriched in sap from R. solanacearum-infected tomato plants.
31765286	1	56	theme	xylem-dwelling	133:146	arg1	solanacearum					173:184	The xylem-dwelling plant pathogen Ralstonia solanacearum	129:184	The xylem-dwelling plant pathogen Ralstonia solanacearum	129:184	The xylem-dwelling plant pathogen Ralstonia solanacearum changes the chemical composition of host xylem sap during bacterial wilt disease.
31765286	9	57	theme	otsA	1419:1422	arg1	mutant					1464:1469	An otsA (trehalose-6-phosphate synthase) single mutant	1416:1469	An otsA (trehalose-6-phosphate synthase) single mutant	1416:1469	An otsA (trehalose-6-phosphate synthase) single mutant behaved similarly to ΔtreY/treS/otsA in all experimental settings, suggesting that the OtsAB pathway is the dominant trehalose synthesis pathway in R. solanacearum.
31765286	1	58	theme	bacterial	244:252	arg1	disease					259:265	bacterial wilt disease	244:265	bacterial wilt disease	244:265	The xylem-dwelling plant pathogen Ralstonia solanacearum changes the chemical composition of host xylem sap during bacterial wilt disease.
31765286	7	59	theme	soil-soak	1039:1047	arg1	inoculation					1049:1059	naturalistic soil-soak inoculation	1026:1059	naturalistic soil-soak inoculation of tomato plants	1026:1076	Following naturalistic soil-soak inoculation of tomato plants, this triple mutant did not cause disease as well as wild-type R. solanacearum.
31765286	8	60	theme	wild-type	1171:1179	arg1	strain					1181:1186	the wild-type strain	1167:1186	the wild-type strain	1167:1186	Further, the wild-type strain out-competed the trehalose-nonproducing mutant by over 600-fold when tomato plants were coinoculated with both strains, showing that trehalose biosynthesis helps R. solanacearum overcome environmental stresses during infection.
31765286	9	61	from	ΔtreY/treS/otsA	1492:1506	arg1	settings					1528:1535	all experimental settings	1511:1535	all experimental settings	1511:1535	An otsA (trehalose-6-phosphate synthase) single mutant behaved similarly to ΔtreY/treS/otsA in all experimental settings, suggesting that the OtsAB pathway is the dominant trehalose synthesis pathway in R. solanacearum.
31765286	1	62	theme	plant	148:152	arg1	solanacearum					173:184	The xylem-dwelling plant pathogen Ralstonia solanacearum	129:184	The xylem-dwelling plant pathogen Ralstonia solanacearum	129:184	The xylem-dwelling plant pathogen Ralstonia solanacearum changes the chemical composition of host xylem sap during bacterial wilt disease.
31765286	1	63	theme	wilt	254:257	arg1	disease					259:265	bacterial wilt disease	244:265	bacterial wilt disease	244:265	The xylem-dwelling plant pathogen Ralstonia solanacearum changes the chemical composition of host xylem sap during bacterial wilt disease.
31765286	2	64	theme	disaccharide	272:283	arg1	trehalose					285:293	The disaccharide trehalose	268:293	The disaccharide trehalose	268:293	The disaccharide trehalose, implicated in stress tolerance across all kingdoms of life, is enriched in sap from R. solanacearum-infected tomato plants.
31765286	5	65	theme	intracellular	797:809	arg1	trehalose					811:819	30-fold less intracellular trehalose	784:819	30-fold less intracellular trehalose	784:819	A quadruple treY/treS/otsA/treA mutant produced 30-fold less intracellular trehalose than the wild-type strain missing the trehalase enzyme.
31765286	2	66	theme	R.	380:381	arg1	plants					412:417	R. solanacearum-infected tomato plants	380:417	R. solanacearum-infected tomato plants	380:417	The disaccharide trehalose, implicated in stress tolerance across all kingdoms of life, is enriched in sap from R. solanacearum-infected tomato plants.
31765286	6	67	theme	trehalose-nonproducing	882:903	arg1	mutant					905:910	This trehalose-nonproducing mutant	877:910	This trehalose-nonproducing mutant	877:910	This trehalose-nonproducing mutant had reduced tolerance to osmotic stress, which the bacterium likely experiences in plant xylem vessels.
31765286	3	68	from	Trehalose	420:428	arg1	sap					439:441	xylem sap	433:441	xylem sap	433:441	Trehalose in xylem sap could be synthesized by the bacterium, the plant, or both.
31765286	4	69	dep	pathways	649:656	arg1	TreS					682:685	TreS	682:685	TreS	682:685	To investigate the source and role of trehalose metabolism during wilt disease, we evaluated the effects of deleting the three trehalose synthesis pathways in the pathogen: TreYZ, TreS, and OtsAB, as well as its sole trehalase, TreA.
31765286	4	69	dep	pathways	649:656	arg1	OtsAB					692:696	OtsAB	692:696	OtsAB	692:696	To investigate the source and role of trehalose metabolism during wilt disease, we evaluated the effects of deleting the three trehalose synthesis pathways in the pathogen: TreYZ, TreS, and OtsAB, as well as its sole trehalase, TreA.
31765286	4	69	dep	pathways	649:656	arg1	TreYZ					675:679	TreYZ	675:679	TreYZ	675:679	To investigate the source and role of trehalose metabolism during wilt disease, we evaluated the effects of deleting the three trehalose synthesis pathways in the pathogen: TreYZ, TreS, and OtsAB, as well as its sole trehalase, TreA.
31765286	4	69	dep	pathways	649:656	arg1	pathways					649:656	the three trehalose synthesis pathways	619:656	the three trehalose synthesis pathways in the pathogen: TreYZ, TreS, and OtsAB	619:696	To investigate the source and role of trehalose metabolism during wilt disease, we evaluated the effects of deleting the three trehalose synthesis pathways in the pathogen: TreYZ, TreS, and OtsAB, as well as its sole trehalase, TreA.
31765286	1	70	theme	Ralstonia	163:171	arg1	solanacearum					173:184	The xylem-dwelling plant pathogen Ralstonia solanacearum	129:184	The xylem-dwelling plant pathogen Ralstonia solanacearum	129:184	The xylem-dwelling plant pathogen Ralstonia solanacearum changes the chemical composition of host xylem sap during bacterial wilt disease.
31765286	6	71	theme	osmotic	937:943	arg1	stress					945:950	osmotic stress	937:950	osmotic stress	937:950	This trehalose-nonproducing mutant had reduced tolerance to osmotic stress, which the bacterium likely experiences in plant xylem vessels.
31765286	6	72	theme	xylem	1001:1005	arg1	vessels					1007:1013	plant xylem vessels	995:1013	plant xylem vessels	995:1013	This trehalose-nonproducing mutant had reduced tolerance to osmotic stress, which the bacterium likely experiences in plant xylem vessels.
32899169	5	0	theme	properties	665:674	arg1	comparison					676:685	properties comparison	665:685	properties comparison	665:685	Nanofibers made of the polyelectrolyte complex (PEC) were successfully manufactured by electrospinning, and casted films were used as a model for properties comparison.
32899169	10	1	theme	formic/water	1328:1339	arg1	solvent					1391:1397	the more appropriated solvent	1369:1397	the more appropriated solvent for the carried-out procedures	1369:1428	Acid formic/water 50/50 v/v is observed to be the more appropriated solvent for the carried-out procedures.
32899169	10	1	theme	formic/water	1328:1339	arg1	v/v					1347:1349	Acid formic/water 50/50 v/v	1323:1349	Acid formic/water 50/50 v/v	1323:1349	Acid formic/water 50/50 v/v is observed to be the more appropriated solvent for the carried-out procedures.
32899169	8	2	theme	swelling	1122:1129	arg1	diameters					1155:1163	diameters	1155:1163	diameters of nanofibers and mechanical performances	1155:1205	The properties of the materials are tested for different PEC compositions at different pH values, based on swelling and solubility degrees, diameters of nanofibers and mechanical performances.
32899169	8	2	theme	swelling	1122:1129	arg1	degrees					1146:1152	swelling and solubility degrees	1122:1152	swelling and solubility degrees	1122:1152	The properties of the materials are tested for different PEC compositions at different pH values, based on swelling and solubility degrees, diameters of nanofibers and mechanical performances.
32899169	9	3	theme	acidic	1238:1243	arg1	potential					1245:1253	acidic potential	1238:1253	acidic potential	1238:1253	The influence of the solvent (acidic potential and composition) utilized to process biomaterials is also examined.
32899169	5	4	theme	casted	627:632	arg1	model					655:659	a model	653:659	a model for properties comparison	653:685	Nanofibers made of the polyelectrolyte complex (PEC) were successfully manufactured by electrospinning, and casted films were used as a model for properties comparison.
32899169	5	4	theme	casted	627:632	arg1	films					634:638	casted films	627:638	casted films	627:638	Nanofibers made of the polyelectrolyte complex (PEC) were successfully manufactured by electrospinning, and casted films were used as a model for properties comparison.
32899169	4	5	dep	engineering	475:485	arg1	applications					505:516	applications	505:516	applications	505:516	These materials will be tested for chondrocyte development in tissue engineering and wound healing applications.
32899169	3	6	theme	matrix	382:387	arg1	structure					395:403	the extracellular matrix (ECM) structure	364:403	the extracellular matrix (ECM) structure	364:403	The objective was to produce new biomaterials being biocompatible and bioresorbable in the body as well as approaching the extracellular matrix (ECM) structure.
32899169	10	7	theme	carried-out	1407:1417	arg1	procedures					1419:1428	the carried-out procedures	1403:1428	the carried-out procedures	1403:1428	Acid formic/water 50/50 v/v is observed to be the more appropriated solvent for the carried-out procedures.
32899169	1	8	theme	Physical	75:82	arg1	Properties					84:93	Physical Properties	75:93	Physical Properties	75:93	Characterization and Physical Properties as a Function of the Composition.
32899169	3	9	from	biocompatible	297:309	arg1	body					336:339	the body	332:339	the body as well as approaching the extracellular matrix (ECM) structure	332:403	The objective was to produce new biomaterials being biocompatible and bioresorbable in the body as well as approaching the extracellular matrix (ECM) structure.
32899169	7	10	theme	polysaccharides	961:975	arg1	crosslinking					937:948	crosslinking	937:948	crosslinking of the two polysaccharides by H-bonds and amide bonds formation	937:1012	The role of thermal treatment at 120 °C for 4 h is examined to control the degree of swelling by crosslinking of the two polysaccharides by H-bonds and amide bonds formation.
32899169	7	11	theme	swelling	925:932	arg1	degree					915:920	the degree	911:920	the degree of swelling	911:932	The role of thermal treatment at 120 °C for 4 h is examined to control the degree of swelling by crosslinking of the two polysaccharides by H-bonds and amide bonds formation.
32899169	7	12	from	°C	877:878	arg1	role					844:847	The role	840:847	The role of thermal treatment at 120 °C for 4 h	840:886	The role of thermal treatment at 120 °C for 4 h is examined to control the degree of swelling by crosslinking of the two polysaccharides by H-bonds and amide bonds formation.
32899169	7	12	from	°C	877:878	arg1	treatment					860:868	thermal treatment	852:868	thermal treatment at 120 °C	852:878	The role of thermal treatment at 120 °C for 4 h is examined to control the degree of swelling by crosslinking of the two polysaccharides by H-bonds and amide bonds formation.
32899169	7	13	theme	treatment	860:868	arg1	role					844:847	The role	840:847	The role of thermal treatment at 120 °C for 4 h	840:886	The role of thermal treatment at 120 °C for 4 h is examined to control the degree of swelling by crosslinking of the two polysaccharides by H-bonds and amide bonds formation.
32899169	2	14	dep	chitosan	183:190	arg1	complex					223:229	polyelectrolyte complex	207:229	polyelectrolyte complex	207:229	In this work, optimized conditions for preparation of chitosan and hyaluronan polyelectrolyte complex are proposed.
32899169	4	15	theme	wound	491:495	arg1	healing					497:503	wound healing	491:503	wound healing	491:503	These materials will be tested for chondrocyte development in tissue engineering and wound healing applications.
32899169	6	16	theme	first	716:720	arg1	it					706:707	it	706:707	it	706:707	To our knowledge, it is the first time that stable chitosan/hyaluronan fibers are produced, which were observed to be long-lasting in buffer at pH~7.4.
32899169	6	16	theme	first	716:720	arg1	time					722:725	the first time that stable chitosan/hyaluronan fibers are produced, which were observed to be long-lasting in buffer at pH~7.4	712:837	the first time that stable chitosan/hyaluronan fibers are produced, which were observed to be long-lasting in buffer at pH~7.4	712:837	To our knowledge, it is the first time that stable chitosan/hyaluronan fibers are produced, which were observed to be long-lasting in buffer at pH~7.4.
32899169	3	17	theme	extracellular	368:380	arg1	ECM					390:392	ECM	390:392	ECM	390:392	The objective was to produce new biomaterials being biocompatible and bioresorbable in the body as well as approaching the extracellular matrix (ECM) structure.
32899169	3	17	theme	extracellular	368:380	arg1	matrix					382:387	extracellular matrix	368:387	the extracellular matrix (ECM) structure	364:403	The objective was to produce new biomaterials being biocompatible and bioresorbable in the body as well as approaching the extracellular matrix (ECM) structure.
32899169	2	18	theme	optimized	143:151	arg1	conditions					153:162	optimized conditions	143:162	optimized conditions for preparation of chitosan and hyaluronan polyelectrolyte complex	143:229	In this work, optimized conditions for preparation of chitosan and hyaluronan polyelectrolyte complex are proposed.
32899169	3	19	theme	new	274:276	arg1	biomaterials					278:289	new biomaterials	274:289	new biomaterials	274:289	The objective was to produce new biomaterials being biocompatible and bioresorbable in the body as well as approaching the extracellular matrix (ECM) structure.
32899169	6	20	from	long-lasting	806:817	arg1	buffer					822:827	buffer	822:827	buffer at pH~7.4	822:837	To our knowledge, it is the first time that stable chitosan/hyaluronan fibers are produced, which were observed to be long-lasting in buffer at pH~7.4.
32899169	7	21	dep	H-bonds	980:986	arg1	formation					1004:1012	formation	1004:1012	formation	1004:1012	The role of thermal treatment at 120 °C for 4 h is examined to control the degree of swelling by crosslinking of the two polysaccharides by H-bonds and amide bonds formation.
32899169	7	22	theme	thermal	852:858	arg1	treatment					860:868	thermal treatment	852:868	thermal treatment at 120 °C	852:878	The role of thermal treatment at 120 °C for 4 h is examined to control the degree of swelling by crosslinking of the two polysaccharides by H-bonds and amide bonds formation.
32899169	2	23	theme	hyaluronan	196:205	arg1	preparation					168:178	preparation	168:178	preparation of chitosan and hyaluronan polyelectrolyte complex	168:229	In this work, optimized conditions for preparation of chitosan and hyaluronan polyelectrolyte complex are proposed.
32899169	10	24	theme	appropriated	1378:1389	arg1	v/v					1347:1349	Acid formic/water 50/50 v/v	1323:1349	Acid formic/water 50/50 v/v	1323:1349	Acid formic/water 50/50 v/v is observed to be the more appropriated solvent for the carried-out procedures.
32899169	10	24	theme	appropriated	1378:1389	arg1	solvent					1391:1397	the more appropriated solvent	1369:1397	the more appropriated solvent for the carried-out procedures	1369:1428	Acid formic/water 50/50 v/v is observed to be the more appropriated solvent for the carried-out procedures.
32899169	5	25	used	used	645:648	arg2	films					634:638	casted films	627:638	casted films	627:638	Nanofibers made of the polyelectrolyte complex (PEC) were successfully manufactured by electrospinning, and casted films were used as a model for properties comparison.
32899169	5	25	used	used	645:648	arg2	model					655:659	a model	653:659	a model for properties comparison	653:685	Nanofibers made of the polyelectrolyte complex (PEC) were successfully manufactured by electrospinning, and casted films were used as a model for properties comparison.
32899169	6	26	from	buffer	822:827	arg1	long-lasting					806:817	long-lasting	806:817	long-lasting	806:817	To our knowledge, it is the first time that stable chitosan/hyaluronan fibers are produced, which were observed to be long-lasting in buffer at pH~7.4.
32899169	0	27	theme	Complex	34:40	arg1	Nanofibers					42:51	Chitosan/Hyaluronan Complex Nanofibers	14:51	Chitosan/Hyaluronan Complex Nanofibers	14:51	Production of Chitosan/Hyaluronan Complex Nanofibers.
32899169	1	28	dep	Characterization	54:69	arg1	Function					100:107	a Function	98:107	a Function of the Composition	98:126	Characterization and Physical Properties as a Function of the Composition.
32899169	0	29	theme	Chitosan/Hyaluronan	14:32	arg1	Nanofibers					42:51	Chitosan/Hyaluronan Complex Nanofibers	14:51	Chitosan/Hyaluronan Complex Nanofibers	14:51	Production of Chitosan/Hyaluronan Complex Nanofibers.
32899169	4	30	theme	tissue	468:473	arg1	engineering					475:485	tissue engineering	468:485	tissue engineering	468:485	These materials will be tested for chondrocyte development in tissue engineering and wound healing applications.
32899169	1	31	theme	Composition	116:126	arg1	Function					100:107	a Function	98:107	a Function of the Composition	98:126	Characterization and Physical Properties as a Function of the Composition.
32899169	8	32	theme	mechanical	1183:1192	arg1	performances					1194:1205	mechanical performances	1183:1205	mechanical performances	1183:1205	The properties of the materials are tested for different PEC compositions at different pH values, based on swelling and solubility degrees, diameters of nanofibers and mechanical performances.
32899169	8	33	theme	PEC	1072:1074	arg1	compositions					1076:1087	different PEC compositions	1062:1087	different PEC compositions	1062:1087	The properties of the materials are tested for different PEC compositions at different pH values, based on swelling and solubility degrees, diameters of nanofibers and mechanical performances.
32899169	2	34	theme	chitosan	183:190	arg1	preparation					168:178	preparation	168:178	preparation of chitosan and hyaluronan polyelectrolyte complex	168:229	In this work, optimized conditions for preparation of chitosan and hyaluronan polyelectrolyte complex are proposed.
32899169	0	35	theme	Nanofibers	42:51	arg1	Production					0:9	Production	0:9	Production of Chitosan/Hyaluronan Complex Nanofibers.	0:52	Production of Chitosan/Hyaluronan Complex Nanofibers.
32899169	6	36	from	pH~7.4	832:837	arg1	buffer					822:827	buffer	822:827	buffer at pH~7.4	822:837	To our knowledge, it is the first time that stable chitosan/hyaluronan fibers are produced, which were observed to be long-lasting in buffer at pH~7.4.
32899169	8	37	theme	nanofibers	1168:1177	arg1	diameters					1155:1163	diameters	1155:1163	diameters of nanofibers and mechanical performances	1155:1205	The properties of the materials are tested for different PEC compositions at different pH values, based on swelling and solubility degrees, diameters of nanofibers and mechanical performances.
32899169	8	37	theme	nanofibers	1168:1177	arg1	degrees					1146:1152	swelling and solubility degrees	1122:1152	swelling and solubility degrees	1122:1152	The properties of the materials are tested for different PEC compositions at different pH values, based on swelling and solubility degrees, diameters of nanofibers and mechanical performances.
32899169	7	38	theme	amide	992:996	arg1	bonds					998:1002	amide bonds	992:1002	amide bonds	992:1002	The role of thermal treatment at 120 °C for 4 h is examined to control the degree of swelling by crosslinking of the two polysaccharides by H-bonds and amide bonds formation.
32899169	8	39	theme	solubility	1135:1144	arg1	diameters					1155:1163	diameters	1155:1163	diameters of nanofibers and mechanical performances	1155:1205	The properties of the materials are tested for different PEC compositions at different pH values, based on swelling and solubility degrees, diameters of nanofibers and mechanical performances.
32899169	8	39	theme	solubility	1135:1144	arg1	degrees					1146:1152	swelling and solubility degrees	1122:1152	swelling and solubility degrees	1122:1152	The properties of the materials are tested for different PEC compositions at different pH values, based on swelling and solubility degrees, diameters of nanofibers and mechanical performances.
32899169	4	40	from	development	453:463	arg1	engineering					475:485	tissue engineering	468:485	tissue engineering	468:485	These materials will be tested for chondrocyte development in tissue engineering and wound healing applications.
32899169	4	40	from	development	453:463	arg1	healing					497:503	wound healing	491:503	wound healing	491:503	These materials will be tested for chondrocyte development in tissue engineering and wound healing applications.
32899169	8	41	from	values	1105:1110	arg1	compositions					1076:1087	different PEC compositions	1062:1087	different PEC compositions	1062:1087	The properties of the materials are tested for different PEC compositions at different pH values, based on swelling and solubility degrees, diameters of nanofibers and mechanical performances.
32899169	8	42	theme	performances	1194:1205	arg1	diameters					1155:1163	diameters	1155:1163	diameters of nanofibers and mechanical performances	1155:1205	The properties of the materials are tested for different PEC compositions at different pH values, based on swelling and solubility degrees, diameters of nanofibers and mechanical performances.
32899169	8	42	theme	performances	1194:1205	arg1	degrees					1146:1152	swelling and solubility degrees	1122:1152	swelling and solubility degrees	1122:1152	The properties of the materials are tested for different PEC compositions at different pH values, based on swelling and solubility degrees, diameters of nanofibers and mechanical performances.
32899169	9	43	theme	solvent	1229:1235	arg1	influence					1212:1220	The influence	1208:1220	The influence of the solvent (acidic potential and composition) utilized to process biomaterials	1208:1303	The influence of the solvent (acidic potential and composition) utilized to process biomaterials is also examined.
32899169	7	44	from	role	844:847	arg1	°C					877:878	120 °C	873:878	120 °C	873:878	The role of thermal treatment at 120 °C for 4 h is examined to control the degree of swelling by crosslinking of the two polysaccharides by H-bonds and amide bonds formation.
32899169	8	45	theme	pH	1102:1103	arg1	values					1105:1110	different pH values	1092:1110	different pH values	1092:1110	The properties of the materials are tested for different PEC compositions at different pH values, based on swelling and solubility degrees, diameters of nanofibers and mechanical performances.
32899169	10	46	theme	50/50	1341:1345	arg1	solvent					1391:1397	the more appropriated solvent	1369:1397	the more appropriated solvent for the carried-out procedures	1369:1428	Acid formic/water 50/50 v/v is observed to be the more appropriated solvent for the carried-out procedures.
32899169	10	46	theme	50/50	1341:1345	arg1	v/v					1347:1349	Acid formic/water 50/50 v/v	1323:1349	Acid formic/water 50/50 v/v	1323:1349	Acid formic/water 50/50 v/v is observed to be the more appropriated solvent for the carried-out procedures.
32899169	8	47	theme	different	1092:1100	arg1	values					1105:1110	different pH values	1092:1110	different pH values	1092:1110	The properties of the materials are tested for different PEC compositions at different pH values, based on swelling and solubility degrees, diameters of nanofibers and mechanical performances.
32899169	2	48	theme	polyelectrolyte	207:221	arg1	complex					223:229	polyelectrolyte complex	207:229	polyelectrolyte complex	207:229	In this work, optimized conditions for preparation of chitosan and hyaluronan polyelectrolyte complex are proposed.
32899169	10	49	theme	Acid	1323:1326	arg1	solvent					1391:1397	the more appropriated solvent	1369:1397	the more appropriated solvent for the carried-out procedures	1369:1428	Acid formic/water 50/50 v/v is observed to be the more appropriated solvent for the carried-out procedures.
32899169	10	49	theme	Acid	1323:1326	arg1	v/v					1347:1349	Acid formic/water 50/50 v/v	1323:1349	Acid formic/water 50/50 v/v	1323:1349	Acid formic/water 50/50 v/v is observed to be the more appropriated solvent for the carried-out procedures.
32899169	5	50	theme	polyelectrolyte	542:556	arg1	PEC					567:569	PEC	567:569	PEC	567:569	Nanofibers made of the polyelectrolyte complex (PEC) were successfully manufactured by electrospinning, and casted films were used as a model for properties comparison.
32899169	5	50	theme	polyelectrolyte	542:556	arg1	complex					558:564	the polyelectrolyte complex	538:564	the polyelectrolyte complex (PEC)	538:570	Nanofibers made of the polyelectrolyte complex (PEC) were successfully manufactured by electrospinning, and casted films were used as a model for properties comparison.
32899169	8	51	theme	materials	1037:1045	arg1	properties					1019:1028	The properties	1015:1028	The properties of the materials	1015:1045	The properties of the materials are tested for different PEC compositions at different pH values, based on swelling and solubility degrees, diameters of nanofibers and mechanical performances.
32899169	4	52	theme	chondrocyte	441:451	arg1	development					453:463	chondrocyte development	441:463	chondrocyte development in tissue engineering and wound healing applications	441:516	These materials will be tested for chondrocyte development in tissue engineering and wound healing applications.
32899169	6	53	theme	chitosan/hyaluronan	739:757	arg1	fibers					759:764	stable chitosan/hyaluronan fibers	732:764	stable chitosan/hyaluronan fibers	732:764	To our knowledge, it is the first time that stable chitosan/hyaluronan fibers are produced, which were observed to be long-lasting in buffer at pH~7.4.
32899169	9	54	dep	solvent	1229:1235	arg1	composition					1259:1269	composition	1259:1269	composition	1259:1269	The influence of the solvent (acidic potential and composition) utilized to process biomaterials is also examined.
32899169	9	54	dep	solvent	1229:1235	arg1	potential					1245:1253	acidic potential	1238:1253	acidic potential	1238:1253	The influence of the solvent (acidic potential and composition) utilized to process biomaterials is also examined.
32899169	6	55	theme	stable	732:737	arg1	fibers					759:764	stable chitosan/hyaluronan fibers	732:764	stable chitosan/hyaluronan fibers	732:764	To our knowledge, it is the first time that stable chitosan/hyaluronan fibers are produced, which were observed to be long-lasting in buffer at pH~7.4.
32899169	8	56	theme	different	1062:1070	arg1	compositions					1076:1087	different PEC compositions	1062:1087	different PEC compositions	1062:1087	The properties of the materials are tested for different PEC compositions at different pH values, based on swelling and solubility degrees, diameters of nanofibers and mechanical performances.
32899169	3	57	from	body	336:339	arg1	biocompatible					297:309	biocompatible	297:309	biocompatible	297:309	The objective was to produce new biomaterials being biocompatible and bioresorbable in the body as well as approaching the extracellular matrix (ECM) structure.
35010897	6	0	theme	male	787:790	arg1	rats					792:795	male rats	787:795	male rats fed high-fat-sucrose diet	787:821	We showed that male rats fed high-fat-sucrose diet developed gut microbiota dysbiosis, increased in body fat, decreased antioxidant activity, decreased brain neuropeptide Y, increased the number of astrocytes and activated microglia, along with reduced spine density associated with deficits in working memory.
35010897	7	1	theme	nopal	1113:1117	arg1	combination					1098:1108	a combination	1096:1108	a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months	1096:1194	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	8	2	theme	TLR4-TNFα	1550:1558	arg1	pathway					1560:1566	the TLR4-TNFα pathway	1546:1566	the TLR4-TNFα pathway	1546:1566	In the liver, ingestion of bioactive food significantly increased antioxidant enzymes, decreased lipogenesis, reduced inflammation mediated by the TLR4-TNFα pathway along with a decrease in body fat, glucose intolerance, and metabolic inflexibility.
35010897	7	3	theme	serum	1300:1304	arg1	levels					1310:1315	serum LPS levels	1300:1315	serum LPS levels	1300:1315	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	4	4	theme	liver	564:568	arg1	samples					606:612	liver, serum, feces, intestine, and brain samples	564:612	liver, serum, feces, intestine, and brain samples	564:612	At the end of this period, liver, serum, feces, intestine, and brain samples were taken.
35010897	10	5	theme	bioactive	1777:1785	arg1	foods					1787:1791	bioactive foods	1777:1791	bioactive foods	1777:1791	Our study demonstrates that consumption of bioactive foods was associated with reduced liver, brain, and gut microbiota alterations in obese rats.
35010897	1	6	from	changes	367:373	arg1	gut					378:380	gut	378:380	gut	378:380	Obesity is associated with cognitive deficit and liver alterations; however, it remains unclear whether a combination of functional foods could reverse cognitive damage and to what extent it would be associated with changes in gut microbiota and liver.
35010897	1	6	from	changes	367:373	arg1	liver					397:401	liver	397:401	liver	397:401	Obesity is associated with cognitive deficit and liver alterations; however, it remains unclear whether a combination of functional foods could reverse cognitive damage and to what extent it would be associated with changes in gut microbiota and liver.
35010897	1	7	theme	cognitive	178:186	arg1	deficit					188:194	cognitive deficit	178:194	cognitive deficit	178:194	Obesity is associated with cognitive deficit and liver alterations; however, it remains unclear whether a combination of functional foods could reverse cognitive damage and to what extent it would be associated with changes in gut microbiota and liver.
35010897	4	8	theme	serum	571:575	arg1	samples					606:612	liver, serum, feces, intestine, and brain samples	564:612	liver, serum, feces, intestine, and brain samples	564:612	At the end of this period, liver, serum, feces, intestine, and brain samples were taken.
35010897	9	9	from	neuroinflammation	1662:1678	arg1	brain					1687:1691	the brain	1683:1691	the brain	1683:1691	Finally, neuroinflammation in the brain was reduced and working memory improved.
35010897	10	10	theme	liver	1821:1825	arg1	alterations					1854:1864	reduced liver, brain, and gut microbiota alterations	1813:1864	reduced liver, brain, and gut microbiota alterations in obese rats	1813:1878	Our study demonstrates that consumption of bioactive foods was associated with reduced liver, brain, and gut microbiota alterations in obese rats.
35010897	7	11	with	acid	1359:1362	arg1	properties					1391:1400	neuroprotective properties	1375:1400	neuroprotective properties	1375:1400	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	6	12	theme	reduced	1017:1023	arg1	density					1031:1037	reduced spine density	1017:1037	reduced spine density associated with deficits in working memory	1017:1080	We showed that male rats fed high-fat-sucrose diet developed gut microbiota dysbiosis, increased in body fat, decreased antioxidant activity, decreased brain neuropeptide Y, increased the number of astrocytes and activated microglia, along with reduced spine density associated with deficits in working memory.
35010897	0	13	theme	Microbiota	115:124	arg1	Restoration					96:106	Restoration	96:106	Restoration of Gut Microbiota and Antioxidant Enzymes	96:148	Bioactive Foods Decrease Liver and Brain Alterations Induced by a High-Fat-Sucrose Diet through Restoration of Gut Microbiota and Antioxidant Enzymes.
35010897	6	14	theme	gut	833:835	arg1	dysbiosis					848:856	gut microbiota dysbiosis	833:856	gut microbiota dysbiosis	833:856	We showed that male rats fed high-fat-sucrose diet developed gut microbiota dysbiosis, increased in body fat, decreased antioxidant activity, decreased brain neuropeptide Y, increased the number of astrocytes and activated microglia, along with reduced spine density associated with deficits in working memory.
35010897	4	15	theme	period	556:561	arg1	end					544:546	the end	540:546	the end of this period	540:561	At the end of this period, liver, serum, feces, intestine, and brain samples were taken.
35010897	0	16	theme	Antioxidant	130:140	arg1	Enzymes					142:148	Antioxidant Enzymes	130:148	Antioxidant Enzymes	130:148	Bioactive Foods Decrease Liver and Brain Alterations Induced by a High-Fat-Sucrose Diet through Restoration of Gut Microbiota and Antioxidant Enzymes.
35010897	3	17	theme	bioactive	520:528	arg1	foods					530:534	bioactive foods	520:534	bioactive foods	520:534	And were then fed for 1 mo. with bioactive foods.
35010897	5	18	theme	glucose	695:701	arg1	test					713:716	intraperitoneal glucose tolerance test	679:716	intraperitoneal glucose tolerance test	679:716	Body composition, energy expenditure, LPS, hormones, intraperitoneal glucose tolerance test, behavioral tests, and gut microbiota were evaluated.
35010897	1	19	theme	foods	283:287	arg1	combination					257:267	a combination	255:267	a combination of functional foods	255:287	Obesity is associated with cognitive deficit and liver alterations; however, it remains unclear whether a combination of functional foods could reverse cognitive damage and to what extent it would be associated with changes in gut microbiota and liver.
35010897	10	20	theme	brain	1828:1832	arg1	alterations					1854:1864	reduced liver, brain, and gut microbiota alterations	1813:1864	reduced liver, brain, and gut microbiota alterations in obese rats	1813:1878	Our study demonstrates that consumption of bioactive foods was associated with reduced liver, brain, and gut microbiota alterations in obese rats.
35010897	8	21	theme	glucose	1603:1609	arg1	intolerance					1611:1621	glucose intolerance	1603:1621	glucose intolerance	1603:1621	In the liver, ingestion of bioactive food significantly increased antioxidant enzymes, decreased lipogenesis, reduced inflammation mediated by the TLR4-TNFα pathway along with a decrease in body fat, glucose intolerance, and metabolic inflexibility.
35010897	6	22	theme	antioxidant	892:902	arg1	fat					877:879	body fat	872:879	body fat	872:879	We showed that male rats fed high-fat-sucrose diet developed gut microbiota dysbiosis, increased in body fat, decreased antioxidant activity, decreased brain neuropeptide Y, increased the number of astrocytes and activated microglia, along with reduced spine density associated with deficits in working memory.
35010897	6	22	theme	antioxidant	892:902	arg1	activity					904:911	decreased antioxidant activity	882:911	decreased antioxidant activity	882:911	We showed that male rats fed high-fat-sucrose diet developed gut microbiota dysbiosis, increased in body fat, decreased antioxidant activity, decreased brain neuropeptide Y, increased the number of astrocytes and activated microglia, along with reduced spine density associated with deficits in working memory.
35010897	7	23	theme	oil	1157:1159	arg1	combination					1098:1108	a combination	1096:1108	a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months	1096:1194	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	4	24	theme	feces	578:582	arg1	samples					606:612	liver, serum, feces, intestine, and brain samples	564:612	liver, serum, feces, intestine, and brain samples	564:612	At the end of this period, liver, serum, feces, intestine, and brain samples were taken.
35010897	10	25	theme	microbiota	1843:1852	arg1	alterations					1854:1864	reduced liver, brain, and gut microbiota alterations	1813:1864	reduced liver, brain, and gut microbiota alterations in obese rats	1813:1878	Our study demonstrates that consumption of bioactive foods was associated with reduced liver, brain, and gut microbiota alterations in obese rats.
35010897	7	26	with	bacteria	1244:1251	arg1	capacity					1276:1283	anti-inflammatory capacity	1258:1283	anti-inflammatory capacity	1258:1283	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	8	27	theme	metabolic	1628:1636	arg1	inflexibility					1638:1650	metabolic inflexibility	1628:1650	metabolic inflexibility	1628:1650	In the liver, ingestion of bioactive food significantly increased antioxidant enzymes, decreased lipogenesis, reduced inflammation mediated by the TLR4-TNFα pathway along with a decrease in body fat, glucose intolerance, and metabolic inflexibility.
35010897	2	28	theme	%	457:457	arg1	HFS					472:474	HFS	472:474	HFS	472:474	With this aim, male Wistar rats were fed a high-fat-5%sucrose diet (HFS) for 4 mo.
35010897	2	28	theme	%	457:457	arg1	diet					466:469	a high-fat-5%sucrose diet	445:469	a high-fat-5%sucrose diet (HFS)	445:475	With this aim, male Wistar rats were fed a high-fat-5%sucrose diet (HFS) for 4 mo.
35010897	4	29	theme	intestine	585:593	arg1	samples					606:612	liver, serum, feces, intestine, and brain samples	564:612	liver, serum, feces, intestine, and brain samples	564:612	At the end of this period, liver, serum, feces, intestine, and brain samples were taken.
35010897	0	30	theme	High-Fat-Sucrose	66:81	arg1	Diet					83:86	a High-Fat-Sucrose Diet	64:86	a High-Fat-Sucrose Diet	64:86	Bioactive Foods Decrease Liver and Brain Alterations Induced by a High-Fat-Sucrose Diet through Restoration of Gut Microbiota and Antioxidant Enzymes.
35010897	8	31	dep	increased	1459:1467	arg1	decreased					1490:1498	decreased	1490:1498	decreased lipogenesis	1490:1510	In the liver, ingestion of bioactive food significantly increased antioxidant enzymes, decreased lipogenesis, reduced inflammation mediated by the TLR4-TNFα pathway along with a decrease in body fat, glucose intolerance, and metabolic inflexibility.
35010897	8	31	dep	increased	1459:1467	arg1	reduced					1513:1519	reduced	1513:1519	reduced inflammation mediated by the TLR4-TNFα pathway along with a decrease in body fat, glucose intolerance, and metabolic inflexibility	1513:1650	In the liver, ingestion of bioactive food significantly increased antioxidant enzymes, decreased lipogenesis, reduced inflammation mediated by the TLR4-TNFα pathway along with a decrease in body fat, glucose intolerance, and metabolic inflexibility.
35010897	6	32	theme	body	872:875	arg1	fat					877:879	body fat	872:879	body fat	872:879	We showed that male rats fed high-fat-sucrose diet developed gut microbiota dysbiosis, increased in body fat, decreased antioxidant activity, decreased brain neuropeptide Y, increased the number of astrocytes and activated microglia, along with reduced spine density associated with deficits in working memory.
35010897	6	32	theme	body	872:875	arg1	Y					943:943	decreased brain neuropeptide Y	914:943	decreased brain neuropeptide Y	914:943	We showed that male rats fed high-fat-sucrose diet developed gut microbiota dysbiosis, increased in body fat, decreased antioxidant activity, decreased brain neuropeptide Y, increased the number of astrocytes and activated microglia, along with reduced spine density associated with deficits in working memory.
35010897	6	32	theme	body	872:875	arg1	activity					904:911	decreased antioxidant activity	882:911	decreased antioxidant activity	882:911	We showed that male rats fed high-fat-sucrose diet developed gut microbiota dysbiosis, increased in body fat, decreased antioxidant activity, decreased brain neuropeptide Y, increased the number of astrocytes and activated microglia, along with reduced spine density associated with deficits in working memory.
35010897	7	33	theme	neuroprotective	1375:1389	arg1	properties					1391:1400	neuroprotective properties	1375:1400	neuroprotective properties	1375:1400	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	5	34	theme	Body	626:629	arg1	composition					631:641	Body composition	626:641	Body composition	626:641	Body composition, energy expenditure, LPS, hormones, intraperitoneal glucose tolerance test, behavioral tests, and gut microbiota were evaluated.
35010897	2	35	theme	Wistar	424:429	arg1	rats					431:434	male Wistar rats	419:434	male Wistar rats	419:434	With this aim, male Wistar rats were fed a high-fat-5%sucrose diet (HFS) for 4 mo.
35010897	6	36	from	deficits	1055:1062	arg1	memory					1075:1080	working memory	1067:1080	working memory	1067:1080	We showed that male rats fed high-fat-sucrose diet developed gut microbiota dysbiosis, increased in body fat, decreased antioxidant activity, decreased brain neuropeptide Y, increased the number of astrocytes and activated microglia, along with reduced spine density associated with deficits in working memory.
35010897	0	37	theme	Bioactive	0:8	arg1	Foods					10:14	Bioactive Foods	0:14	Bioactive Foods	0:14	Bioactive Foods Decrease Liver and Brain Alterations Induced by a High-Fat-Sucrose Diet through Restoration of Gut Microbiota and Antioxidant Enzymes.
35010897	1	38	theme	functional	272:281	arg1	foods					283:287	functional foods	272:287	functional foods	272:287	Obesity is associated with cognitive deficit and liver alterations; however, it remains unclear whether a combination of functional foods could reverse cognitive damage and to what extent it would be associated with changes in gut microbiota and liver.
35010897	5	39	theme	tolerance	703:711	arg1	test					713:716	intraperitoneal glucose tolerance test	679:716	intraperitoneal glucose tolerance test	679:716	Body composition, energy expenditure, LPS, hormones, intraperitoneal glucose tolerance test, behavioral tests, and gut microbiota were evaluated.
35010897	7	40	from	increase	1219:1226	arg1	cluster					1233:1239	a cluster	1231:1239	a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties	1231:1400	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	6	41	theme	brain	924:928	arg1	fat					877:879	body fat	872:879	body fat	872:879	We showed that male rats fed high-fat-sucrose diet developed gut microbiota dysbiosis, increased in body fat, decreased antioxidant activity, decreased brain neuropeptide Y, increased the number of astrocytes and activated microglia, along with reduced spine density associated with deficits in working memory.
35010897	6	41	theme	brain	924:928	arg1	Y					943:943	decreased brain neuropeptide Y	914:943	decreased brain neuropeptide Y	914:943	We showed that male rats fed high-fat-sucrose diet developed gut microbiota dysbiosis, increased in body fat, decreased antioxidant activity, decreased brain neuropeptide Y, increased the number of astrocytes and activated microglia, along with reduced spine density associated with deficits in working memory.
35010897	6	42	theme	decreased	914:922	arg1	fat					877:879	body fat	872:879	body fat	872:879	We showed that male rats fed high-fat-sucrose diet developed gut microbiota dysbiosis, increased in body fat, decreased antioxidant activity, decreased brain neuropeptide Y, increased the number of astrocytes and activated microglia, along with reduced spine density associated with deficits in working memory.
35010897	6	42	theme	decreased	914:922	arg1	Y					943:943	decreased brain neuropeptide Y	914:943	decreased brain neuropeptide Y	914:943	We showed that male rats fed high-fat-sucrose diet developed gut microbiota dysbiosis, increased in body fat, decreased antioxidant activity, decreased brain neuropeptide Y, increased the number of astrocytes and activated microglia, along with reduced spine density associated with deficits in working memory.
35010897	1	43	theme	cognitive	303:311	arg1	damage					313:318	cognitive damage	303:318	cognitive damage	303:318	Obesity is associated with cognitive deficit and liver alterations; however, it remains unclear whether a combination of functional foods could reverse cognitive damage and to what extent it would be associated with changes in gut microbiota and liver.
35010897	10	44	from	alterations	1854:1864	arg1	rats					1875:1878	obese rats	1869:1878	obese rats	1869:1878	Our study demonstrates that consumption of bioactive foods was associated with reduced liver, brain, and gut microbiota alterations in obese rats.
35010897	7	45	theme	seed	1152:1155	arg1	oil					1157:1159	chia seed oil	1147:1159	chia seed oil (bioactive foods)	1147:1177	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	7	45	theme	seed	1152:1155	arg1	foods					1172:1176	bioactive foods	1162:1176	bioactive foods	1162:1176	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	7	46	theme	serum	1336:1340	arg1	EPA					1365:1367	EPA	1365:1367	EPA	1365:1367	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	7	46	theme	serum	1336:1340	arg1	acid					1359:1362	serum eicosapentaenoic acid	1336:1362	serum eicosapentaenoic acid (EPA) with neuroprotective properties	1336:1400	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	7	47	theme	curcumin	1133:1140	arg1	combination					1098:1108	a combination	1096:1108	a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months	1096:1194	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	7	48	theme	protein	1124:1130	arg1	combination					1098:1108	a combination	1096:1108	a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months	1096:1194	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	8	49	theme	bioactive	1430:1438	arg1	food					1440:1443	bioactive food	1430:1443	bioactive food	1430:1443	In the liver, ingestion of bioactive food significantly increased antioxidant enzymes, decreased lipogenesis, reduced inflammation mediated by the TLR4-TNFα pathway along with a decrease in body fat, glucose intolerance, and metabolic inflexibility.
35010897	7	50	theme	LPS	1306:1308	arg1	levels					1310:1315	serum LPS levels	1300:1315	serum LPS levels	1300:1315	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	8	51	theme	antioxidant	1469:1479	arg1	enzymes					1481:1487	antioxidant enzymes	1469:1487	antioxidant enzymes	1469:1487	In the liver, ingestion of bioactive food significantly increased antioxidant enzymes, decreased lipogenesis, reduced inflammation mediated by the TLR4-TNFα pathway along with a decrease in body fat, glucose intolerance, and metabolic inflexibility.
35010897	10	52	theme	foods	1787:1791	arg1	consumption					1762:1772	consumption	1762:1772	consumption of bioactive foods	1762:1791	Our study demonstrates that consumption of bioactive foods was associated with reduced liver, brain, and gut microbiota alterations in obese rats.
35010897	7	53	theme	anti-inflammatory	1258:1274	arg1	capacity					1276:1283	anti-inflammatory capacity	1258:1283	anti-inflammatory capacity	1258:1283	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	5	54	theme	gut	741:743	arg1	microbiota					745:754	gut microbiota	741:754	gut microbiota	741:754	Body composition, energy expenditure, LPS, hormones, intraperitoneal glucose tolerance test, behavioral tests, and gut microbiota were evaluated.
35010897	7	55	from	decrease	1288:1295	arg1	levels					1310:1315	serum LPS levels	1300:1315	serum LPS levels	1300:1315	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	7	55	from	decrease	1288:1295	arg1	EPA					1365:1367	EPA	1365:1367	EPA	1365:1367	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	7	55	from	decrease	1288:1295	arg1	acid					1359:1362	serum eicosapentaenoic acid	1336:1362	serum eicosapentaenoic acid (EPA) with neuroprotective properties	1336:1400	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	6	56	theme	spine	1025:1029	arg1	density					1031:1037	reduced spine density	1017:1037	reduced spine density associated with deficits in working memory	1017:1080	We showed that male rats fed high-fat-sucrose diet developed gut microbiota dysbiosis, increased in body fat, decreased antioxidant activity, decreased brain neuropeptide Y, increased the number of astrocytes and activated microglia, along with reduced spine density associated with deficits in working memory.
35010897	10	57	theme	reduced	1813:1819	arg1	alterations					1854:1864	reduced liver, brain, and gut microbiota alterations	1813:1864	reduced liver, brain, and gut microbiota alterations in obese rats	1813:1878	Our study demonstrates that consumption of bioactive foods was associated with reduced liver, brain, and gut microbiota alterations in obese rats.
35010897	1	58	theme	liver	200:204	arg1	alterations					206:216	liver alterations	200:216	liver alterations	200:216	Obesity is associated with cognitive deficit and liver alterations; however, it remains unclear whether a combination of functional foods could reverse cognitive damage and to what extent it would be associated with changes in gut microbiota and liver.
35010897	7	59	theme	bacteria	1244:1251	arg1	cluster					1233:1239	a cluster	1231:1239	a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties	1231:1400	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	6	60	theme	microbiota	837:846	arg1	dysbiosis					848:856	gut microbiota dysbiosis	833:856	gut microbiota dysbiosis	833:856	We showed that male rats fed high-fat-sucrose diet developed gut microbiota dysbiosis, increased in body fat, decreased antioxidant activity, decreased brain neuropeptide Y, increased the number of astrocytes and activated microglia, along with reduced spine density associated with deficits in working memory.
35010897	0	61	theme	Enzymes	142:148	arg1	Restoration					96:106	Restoration	96:106	Restoration of Gut Microbiota and Antioxidant Enzymes	96:148	Bioactive Foods Decrease Liver and Brain Alterations Induced by a High-Fat-Sucrose Diet through Restoration of Gut Microbiota and Antioxidant Enzymes.
35010897	5	62	theme	behavioral	719:728	arg1	tests					730:734	behavioral tests	719:734	behavioral tests	719:734	Body composition, energy expenditure, LPS, hormones, intraperitoneal glucose tolerance test, behavioral tests, and gut microbiota were evaluated.
35010897	6	63	dep	developed	823:831	arg1	increased					859:867	increased	859:867	increased in body fat, decreased antioxidant activity, decreased brain neuropeptide Y	859:943	We showed that male rats fed high-fat-sucrose diet developed gut microbiota dysbiosis, increased in body fat, decreased antioxidant activity, decreased brain neuropeptide Y, increased the number of astrocytes and activated microglia, along with reduced spine density associated with deficits in working memory.
35010897	6	63	dep	developed	823:831	arg1	increased					946:954	increased	946:954	increased	946:954	We showed that male rats fed high-fat-sucrose diet developed gut microbiota dysbiosis, increased in body fat, decreased antioxidant activity, decreased brain neuropeptide Y, increased the number of astrocytes and activated microglia, along with reduced spine density associated with deficits in working memory.
35010897	8	64	theme	body	1593:1596	arg1	fat					1598:1600	body fat	1593:1600	body fat	1593:1600	In the liver, ingestion of bioactive food significantly increased antioxidant enzymes, decreased lipogenesis, reduced inflammation mediated by the TLR4-TNFα pathway along with a decrease in body fat, glucose intolerance, and metabolic inflexibility.
35010897	6	65	theme	high-fat-sucrose	801:816	arg1	diet					818:821	high-fat-sucrose diet	801:821	high-fat-sucrose diet	801:821	We showed that male rats fed high-fat-sucrose diet developed gut microbiota dysbiosis, increased in body fat, decreased antioxidant activity, decreased brain neuropeptide Y, increased the number of astrocytes and activated microglia, along with reduced spine density associated with deficits in working memory.
35010897	4	66	theme	brain	600:604	arg1	samples					606:612	liver, serum, feces, intestine, and brain samples	564:612	liver, serum, feces, intestine, and brain samples	564:612	At the end of this period, liver, serum, feces, intestine, and brain samples were taken.
35010897	7	67	theme	combination	1098:1108	arg1	Ingestion					1083:1091	Ingestion	1083:1091	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months	1083:1194	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	6	68	theme	decreased	882:890	arg1	fat					877:879	body fat	872:879	body fat	872:879	We showed that male rats fed high-fat-sucrose diet developed gut microbiota dysbiosis, increased in body fat, decreased antioxidant activity, decreased brain neuropeptide Y, increased the number of astrocytes and activated microglia, along with reduced spine density associated with deficits in working memory.
35010897	6	68	theme	decreased	882:890	arg1	activity					904:911	decreased antioxidant activity	882:911	decreased antioxidant activity	882:911	We showed that male rats fed high-fat-sucrose diet developed gut microbiota dysbiosis, increased in body fat, decreased antioxidant activity, decreased brain neuropeptide Y, increased the number of astrocytes and activated microglia, along with reduced spine density associated with deficits in working memory.
35010897	2	69	theme	sucrose	458:464	arg1	HFS					472:474	HFS	472:474	HFS	472:474	With this aim, male Wistar rats were fed a high-fat-5%sucrose diet (HFS) for 4 mo.
35010897	2	69	theme	sucrose	458:464	arg1	diet					466:469	a high-fat-5%sucrose diet	445:469	a high-fat-5%sucrose diet (HFS)	445:475	With this aim, male Wistar rats were fed a high-fat-5%sucrose diet (HFS) for 4 mo.
35010897	6	70	theme	working	1067:1073	arg1	memory					1075:1080	working memory	1067:1080	working memory	1067:1080	We showed that male rats fed high-fat-sucrose diet developed gut microbiota dysbiosis, increased in body fat, decreased antioxidant activity, decreased brain neuropeptide Y, increased the number of astrocytes and activated microglia, along with reduced spine density associated with deficits in working memory.
35010897	10	71	theme	gut	1839:1841	arg1	alterations					1854:1864	reduced liver, brain, and gut microbiota alterations	1813:1864	reduced liver, brain, and gut microbiota alterations in obese rats	1813:1878	Our study demonstrates that consumption of bioactive foods was associated with reduced liver, brain, and gut microbiota alterations in obese rats.
35010897	5	72	theme	energy	644:649	arg1	expenditure					651:661	energy expenditure	644:661	energy expenditure	644:661	Body composition, energy expenditure, LPS, hormones, intraperitoneal glucose tolerance test, behavioral tests, and gut microbiota were evaluated.
35010897	2	73	theme	high-fat-5	447:456	arg1	%					457:457	%	457:457	%	457:457	With this aim, male Wistar rats were fed a high-fat-5%sucrose diet (HFS) for 4 mo.
35010897	6	74	theme	activated	985:993	arg1	microglia					995:1003	activated microglia	985:1003	activated microglia	985:1003	We showed that male rats fed high-fat-sucrose diet developed gut microbiota dysbiosis, increased in body fat, decreased antioxidant activity, decreased brain neuropeptide Y, increased the number of astrocytes and activated microglia, along with reduced spine density associated with deficits in working memory.
35010897	0	75	theme	Gut	111:113	arg1	Microbiota					115:124	Gut Microbiota	111:124	Gut Microbiota	111:124	Bioactive Foods Decrease Liver and Brain Alterations Induced by a High-Fat-Sucrose Diet through Restoration of Gut Microbiota and Antioxidant Enzymes.
35010897	10	76	theme	obese	1869:1873	arg1	rats					1875:1878	obese rats	1869:1878	obese rats	1869:1878	Our study demonstrates that consumption of bioactive foods was associated with reduced liver, brain, and gut microbiota alterations in obese rats.
35010897	7	77	theme	bioactive	1162:1170	arg1	oil					1157:1159	chia seed oil	1147:1159	chia seed oil (bioactive foods)	1147:1177	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	7	77	theme	bioactive	1162:1170	arg1	foods					1172:1176	bioactive foods	1162:1176	bioactive foods	1162:1176	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	2	78	theme	male	419:422	arg1	rats					431:434	male Wistar rats	419:434	male Wistar rats	419:434	With this aim, male Wistar rats were fed a high-fat-5%sucrose diet (HFS) for 4 mo.
35010897	0	79	theme	Liver	25:29	arg1	Alterations					41:51	Liver and Brain Alterations	25:51	Alterations	41:51	Bioactive Foods Decrease Liver and Brain Alterations Induced by a High-Fat-Sucrose Diet through Restoration of Gut Microbiota and Antioxidant Enzymes.
35010897	5	80	theme	intraperitoneal	679:693	arg1	test					713:716	intraperitoneal glucose tolerance test	679:716	intraperitoneal glucose tolerance test	679:716	Body composition, energy expenditure, LPS, hormones, intraperitoneal glucose tolerance test, behavioral tests, and gut microbiota were evaluated.
35010897	6	81	theme	neuropeptide	930:941	arg1	fat					877:879	body fat	872:879	body fat	872:879	We showed that male rats fed high-fat-sucrose diet developed gut microbiota dysbiosis, increased in body fat, decreased antioxidant activity, decreased brain neuropeptide Y, increased the number of astrocytes and activated microglia, along with reduced spine density associated with deficits in working memory.
35010897	6	81	theme	neuropeptide	930:941	arg1	Y					943:943	decreased brain neuropeptide Y	914:943	decreased brain neuropeptide Y	914:943	We showed that male rats fed high-fat-sucrose diet developed gut microbiota dysbiosis, increased in body fat, decreased antioxidant activity, decreased brain neuropeptide Y, increased the number of astrocytes and activated microglia, along with reduced spine density associated with deficits in working memory.
35010897	0	82	theme	Brain	35:39	arg1	Alterations					41:51	Liver and Brain Alterations	25:51	Alterations	41:51	Bioactive Foods Decrease Liver and Brain Alterations Induced by a High-Fat-Sucrose Diet through Restoration of Gut Microbiota and Antioxidant Enzymes.
35010897	7	83	theme	chia	1147:1150	arg1	oil					1157:1159	chia seed oil	1147:1159	chia seed oil (bioactive foods)	1147:1177	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	7	83	theme	chia	1147:1150	arg1	foods					1172:1176	bioactive foods	1162:1176	bioactive foods	1162:1176	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	7	84	theme	eicosapentaenoic	1342:1357	arg1	EPA					1365:1367	EPA	1365:1367	EPA	1365:1367	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	7	84	theme	eicosapentaenoic	1342:1357	arg1	acid					1359:1362	serum eicosapentaenoic acid	1336:1362	serum eicosapentaenoic acid (EPA) with neuroprotective properties	1336:1400	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	6	85	theme	microglia	995:1003	arg1	number					960:965	the number	956:965	the number of astrocytes and activated microglia	956:1003	We showed that male rats fed high-fat-sucrose diet developed gut microbiota dysbiosis, increased in body fat, decreased antioxidant activity, decreased brain neuropeptide Y, increased the number of astrocytes and activated microglia, along with reduced spine density associated with deficits in working memory.
35010897	8	86	from	decrease	1581:1588	arg1	inflexibility					1638:1650	metabolic inflexibility	1628:1650	metabolic inflexibility	1628:1650	In the liver, ingestion of bioactive food significantly increased antioxidant enzymes, decreased lipogenesis, reduced inflammation mediated by the TLR4-TNFα pathway along with a decrease in body fat, glucose intolerance, and metabolic inflexibility.
35010897	8	86	from	decrease	1581:1588	arg1	fat					1598:1600	body fat	1593:1600	body fat	1593:1600	In the liver, ingestion of bioactive food significantly increased antioxidant enzymes, decreased lipogenesis, reduced inflammation mediated by the TLR4-TNFα pathway along with a decrease in body fat, glucose intolerance, and metabolic inflexibility.
35010897	8	86	from	decrease	1581:1588	arg1	intolerance					1611:1621	glucose intolerance	1603:1621	glucose intolerance	1603:1621	In the liver, ingestion of bioactive food significantly increased antioxidant enzymes, decreased lipogenesis, reduced inflammation mediated by the TLR4-TNFα pathway along with a decrease in body fat, glucose intolerance, and metabolic inflexibility.
35010897	6	87	theme	astrocytes	970:979	arg1	number					960:965	the number	956:965	the number of astrocytes and activated microglia	956:1003	We showed that male rats fed high-fat-sucrose diet developed gut microbiota dysbiosis, increased in body fat, decreased antioxidant activity, decreased brain neuropeptide Y, increased the number of astrocytes and activated microglia, along with reduced spine density associated with deficits in working memory.
35010897	7	88	from	increase	1324:1331	arg1	levels					1310:1315	serum LPS levels	1300:1315	serum LPS levels	1300:1315	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	7	88	from	increase	1324:1331	arg1	EPA					1365:1367	EPA	1365:1367	EPA	1365:1367	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	7	88	from	increase	1324:1331	arg1	acid					1359:1362	serum eicosapentaenoic acid	1336:1362	serum eicosapentaenoic acid (EPA) with neuroprotective properties	1336:1400	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	7	89	theme	soy	1120:1122	arg1	protein					1124:1130	soy protein	1120:1130	soy protein	1120:1130	Ingestion of a combination of nopal, soy protein, curcumin, and chia seed oil (bioactive foods) for three months was associated with an increase in a cluster of bacteria with anti-inflammatory capacity, a decrease in serum LPS levels and an increase in serum eicosapentaenoic acid (EPA) with neuroprotective properties.
35010897	9	90	theme	working	1709:1715	arg1	memory					1717:1722	working memory	1709:1722	working memory	1709:1722	Finally, neuroinflammation in the brain was reduced and working memory improved.
35010897	8	91	theme	food	1440:1443	arg1	ingestion					1417:1425	ingestion	1417:1425	ingestion of bioactive food	1417:1443	In the liver, ingestion of bioactive food significantly increased antioxidant enzymes, decreased lipogenesis, reduced inflammation mediated by the TLR4-TNFα pathway along with a decrease in body fat, glucose intolerance, and metabolic inflexibility.
35010897	1	92	dep	gut	378:380	arg1	microbiota					382:391	microbiota	382:391	microbiota	382:391	Obesity is associated with cognitive deficit and liver alterations; however, it remains unclear whether a combination of functional foods could reverse cognitive damage and to what extent it would be associated with changes in gut microbiota and liver.
33143356	0	0	theme	Sodium	69:74	arg1	Colomast®					22:30	Colomast®	22:30	Colomast®	22:30	Protective effects of Colomast®, A New Formulation of Adelmidrol and Sodium Hyaluronate, in A Mouse Model of Acute Restraint Stress.
33143356	0	0	theme	Sodium	69:74	arg1	Formulation					39:49	A New Formulation	33:49	A New Formulation of Adelmidrol and Sodium Hyaluronate	33:86	Protective effects of Colomast®, A New Formulation of Adelmidrol and Sodium Hyaluronate, in A Mouse Model of Acute Restraint Stress.
33143356	5	1	dep	min	998:1000	arg1	immobilization					1013:1026	the immobilization	1009:1026	the immobilization	1009:1026	Acute restraint stress (ARS) was induced in mice by fixation for 2 h of the four extremities with an adhesive tape and Colomast® (20 mg/kg) was administered by oral gavage 30 min before the immobilization.
33143356	5	2	theme	restraint	829:837	arg1	ARS					847:849	ARS	847:849	ARS	847:849	Acute restraint stress (ARS) was induced in mice by fixation for 2 h of the four extremities with an adhesive tape and Colomast® (20 mg/kg) was administered by oral gavage 30 min before the immobilization.
33143356	5	2	theme	restraint	829:837	arg1	stress					839:844	Acute restraint stress	823:844	Acute restraint stress (ARS)	823:850	Acute restraint stress (ARS) was induced in mice by fixation for 2 h of the four extremities with an adhesive tape and Colomast® (20 mg/kg) was administered by oral gavage 30 min before the immobilization.
33143356	6	3	from	changes	1092:1098	arg1	tract					1124:1128	the gastrointestinal tract	1103:1128	the gastrointestinal tract	1103:1128	Colomast® pre-treatment was able to decrease histopathological changes in the gastrointestinal tract, cytokines expression, neutrophil infiltration, mast cell activation, oxidative stress, as well as modulate nuclear factor NF-kB and apoptosis pathways after ARS induction.
33143356	2	4	theme	risk	444:447	arg1	factor					449:454	a risk factor	442:454	a risk factor for the development of depression and anxiety	442:500	Stress is also known to be a risk factor for the development of depression and anxiety.
33143356	2	4	theme	risk	444:447	arg1	Stress					415:420	Stress	415:420	Stress	415:420	Stress is also known to be a risk factor for the development of depression and anxiety.
33143356	0	5	from	effects	11:17	arg1	Model					100:104	A Mouse Model	92:104	A Mouse Model of Acute Restraint Stress	92:130	Protective effects of Colomast®, A New Formulation of Adelmidrol and Sodium Hyaluronate, in A Mouse Model of Acute Restraint Stress.
33143356	6	6	theme	neutrophil	1153:1162	arg1	infiltration					1164:1175	neutrophil infiltration	1153:1175	neutrophil infiltration	1153:1175	Colomast® pre-treatment was able to decrease histopathological changes in the gastrointestinal tract, cytokines expression, neutrophil infiltration, mast cell activation, oxidative stress, as well as modulate nuclear factor NF-kB and apoptosis pathways after ARS induction.
33143356	6	6	theme	neutrophil	1153:1162	arg1	changes					1092:1098	histopathological changes	1074:1098	histopathological changes in the gastrointestinal tract	1074:1128	Colomast® pre-treatment was able to decrease histopathological changes in the gastrointestinal tract, cytokines expression, neutrophil infiltration, mast cell activation, oxidative stress, as well as modulate nuclear factor NF-kB and apoptosis pathways after ARS induction.
33143356	6	7	theme	mast	1178:1181	arg1	changes					1092:1098	histopathological changes	1074:1098	histopathological changes in the gastrointestinal tract	1074:1128	Colomast® pre-treatment was able to decrease histopathological changes in the gastrointestinal tract, cytokines expression, neutrophil infiltration, mast cell activation, oxidative stress, as well as modulate nuclear factor NF-kB and apoptosis pathways after ARS induction.
33143356	6	7	theme	mast	1178:1181	arg1	activation					1188:1197	mast cell activation	1178:1197	mast cell activation	1178:1197	Colomast® pre-treatment was able to decrease histopathological changes in the gastrointestinal tract, cytokines expression, neutrophil infiltration, mast cell activation, oxidative stress, as well as modulate nuclear factor NF-kB and apoptosis pathways after ARS induction.
33143356	6	8	theme	apoptosis	1263:1271	arg1	pathways					1273:1280	nuclear factor NF-kB and apoptosis pathways	1238:1280	nuclear factor NF-kB and apoptosis pathways	1238:1280	Colomast® pre-treatment was able to decrease histopathological changes in the gastrointestinal tract, cytokines expression, neutrophil infiltration, mast cell activation, oxidative stress, as well as modulate nuclear factor NF-kB and apoptosis pathways after ARS induction.
33143356	4	9	theme	stress	815:820	arg1	model					791:795	an animal model	781:795	an animal model of immobilization stress	781:820	The aim of this study was to explore the protective effect of Colomast®, a new preparation containing Adelmidrol, an enhancer of physiological of palmitoylethanolamide (PEA), and sodium hyaluronate in an animal model of immobilization stress.
33143356	5	10	theme	adhesive	924:931	arg1	tape					933:936	an adhesive tape	921:936	an adhesive tape	921:936	Acute restraint stress (ARS) was induced in mice by fixation for 2 h of the four extremities with an adhesive tape and Colomast® (20 mg/kg) was administered by oral gavage 30 min before the immobilization.
33143356	6	11	theme	histopathological	1074:1090	arg1	infiltration					1164:1175	neutrophil infiltration	1153:1175	neutrophil infiltration	1153:1175	Colomast® pre-treatment was able to decrease histopathological changes in the gastrointestinal tract, cytokines expression, neutrophil infiltration, mast cell activation, oxidative stress, as well as modulate nuclear factor NF-kB and apoptosis pathways after ARS induction.
33143356	6	11	theme	histopathological	1074:1090	arg1	changes					1092:1098	histopathological changes	1074:1098	histopathological changes in the gastrointestinal tract	1074:1128	Colomast® pre-treatment was able to decrease histopathological changes in the gastrointestinal tract, cytokines expression, neutrophil infiltration, mast cell activation, oxidative stress, as well as modulate nuclear factor NF-kB and apoptosis pathways after ARS induction.
33143356	6	11	theme	histopathological	1074:1090	arg1	activation					1188:1197	mast cell activation	1178:1197	mast cell activation	1178:1197	Colomast® pre-treatment was able to decrease histopathological changes in the gastrointestinal tract, cytokines expression, neutrophil infiltration, mast cell activation, oxidative stress, as well as modulate nuclear factor NF-kB and apoptosis pathways after ARS induction.
33143356	6	11	theme	histopathological	1074:1090	arg1	stress					1210:1215	oxidative stress	1200:1215	oxidative stress	1200:1215	Colomast® pre-treatment was able to decrease histopathological changes in the gastrointestinal tract, cytokines expression, neutrophil infiltration, mast cell activation, oxidative stress, as well as modulate nuclear factor NF-kB and apoptosis pathways after ARS induction.
33143356	6	11	theme	histopathological	1074:1090	arg1	expression					1141:1150	cytokines expression	1131:1150	cytokines expression	1131:1150	Colomast® pre-treatment was able to decrease histopathological changes in the gastrointestinal tract, cytokines expression, neutrophil infiltration, mast cell activation, oxidative stress, as well as modulate nuclear factor NF-kB and apoptosis pathways after ARS induction.
33143356	2	12	theme	depression	479:488	arg1	development					464:474	the development	460:474	the development of depression and anxiety	460:500	Stress is also known to be a risk factor for the development of depression and anxiety.
33143356	1	13	theme	hypothalamic-pituitary-adrenal	323:352	arg1	axis					354:357	the hypothalamic-pituitary-adrenal axis	319:357	the hypothalamic-pituitary-adrenal axis (HPA)	319:363	Stress is generally defined as a homeostatic disruption from actual or implied threats and alters the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones.
33143356	1	13	theme	hypothalamic-pituitary-adrenal	323:352	arg1	HPA					360:362	HPA	360:362	HPA	360:362	Stress is generally defined as a homeostatic disruption from actual or implied threats and alters the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones.
33143356	4	14	theme	immobilization	800:813	arg1	stress					815:820	immobilization stress	800:820	immobilization stress	800:820	The aim of this study was to explore the protective effect of Colomast®, a new preparation containing Adelmidrol, an enhancer of physiological of palmitoylethanolamide (PEA), and sodium hyaluronate in an animal model of immobilization stress.
33143356	1	15	theme	homeostatic	235:245	arg1	balance					247:253	the homeostatic balance	231:253	the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones	231:412	Stress is generally defined as a homeostatic disruption from actual or implied threats and alters the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones.
33143356	1	15	theme	homeostatic	235:245	arg1	disruption					178:187	a homeostatic disruption	164:187	a homeostatic disruption from actual or implied threats	164:218	Stress is generally defined as a homeostatic disruption from actual or implied threats and alters the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones.
33143356	1	15	theme	homeostatic	235:245	arg1	Stress					133:138	Stress	133:138	Stress	133:138	Stress is generally defined as a homeostatic disruption from actual or implied threats and alters the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones.
33143356	6	16	theme	NF-kB	1253:1257	arg1	pathways					1273:1280	nuclear factor NF-kB and apoptosis pathways	1238:1280	nuclear factor NF-kB and apoptosis pathways	1238:1280	Colomast® pre-treatment was able to decrease histopathological changes in the gastrointestinal tract, cytokines expression, neutrophil infiltration, mast cell activation, oxidative stress, as well as modulate nuclear factor NF-kB and apoptosis pathways after ARS induction.
33143356	5	17	theme	oral	983:986	arg1	gavage					988:993	oral gavage	983:993	oral gavage	983:993	Acute restraint stress (ARS) was induced in mice by fixation for 2 h of the four extremities with an adhesive tape and Colomast® (20 mg/kg) was administered by oral gavage 30 min before the immobilization.
33143356	4	18	theme	physiological	709:721	arg1	Adelmidrol					682:691	Adelmidrol	682:691	Adelmidrol	682:691	The aim of this study was to explore the protective effect of Colomast®, a new preparation containing Adelmidrol, an enhancer of physiological of palmitoylethanolamide (PEA), and sodium hyaluronate in an animal model of immobilization stress.
33143356	4	18	theme	physiological	709:721	arg1	enhancer					697:704	an enhancer	694:704	an enhancer of physiological of palmitoylethanolamide (PEA)	694:752	The aim of this study was to explore the protective effect of Colomast®, a new preparation containing Adelmidrol, an enhancer of physiological of palmitoylethanolamide (PEA), and sodium hyaluronate in an animal model of immobilization stress.
33143356	4	18	theme	physiological	709:721	arg1	hyaluronate					766:776	sodium hyaluronate	759:776	sodium hyaluronate	759:776	The aim of this study was to explore the protective effect of Colomast®, a new preparation containing Adelmidrol, an enhancer of physiological of palmitoylethanolamide (PEA), and sodium hyaluronate in an animal model of immobilization stress.
33143356	6	19	theme	factor	1246:1251	arg1	NF-kB					1253:1257	nuclear factor NF-kB	1238:1257	nuclear factor NF-kB	1238:1257	Colomast® pre-treatment was able to decrease histopathological changes in the gastrointestinal tract, cytokines expression, neutrophil infiltration, mast cell activation, oxidative stress, as well as modulate nuclear factor NF-kB and apoptosis pathways after ARS induction.
33143356	7	20	theme	tight	1343:1347	arg1	junction					1349:1356	tight junction	1343:1356	tight junction	1343:1356	Moreover, Colomast® was able to restore tight junction in both ileum and hippocampus and cortex.
33143356	4	21	theme	study	596:600	arg1	aim					584:586	The aim	580:586	The aim of this study	580:600	The aim of this study was to explore the protective effect of Colomast®, a new preparation containing Adelmidrol, an enhancer of physiological of palmitoylethanolamide (PEA), and sodium hyaluronate in an animal model of immobilization stress.
33143356	4	22	contain	containing	671:680	arg2	hyaluronate					766:776	sodium hyaluronate	759:776	sodium hyaluronate	759:776	The aim of this study was to explore the protective effect of Colomast®, a new preparation containing Adelmidrol, an enhancer of physiological of palmitoylethanolamide (PEA), and sodium hyaluronate in an animal model of immobilization stress.
33143356	4	22	contain	containing	671:680	arg1	preparation					659:669	a new preparation	653:669	a new preparation containing Adelmidrol, an enhancer of physiological of palmitoylethanolamide (PEA), and sodium hyaluronate in an animal model of immobilization stress	653:820	The aim of this study was to explore the protective effect of Colomast®, a new preparation containing Adelmidrol, an enhancer of physiological of palmitoylethanolamide (PEA), and sodium hyaluronate in an animal model of immobilization stress.
33143356	4	22	contain	containing	671:680	arg2	enhancer					697:704	an enhancer	694:704	an enhancer of physiological of palmitoylethanolamide (PEA)	694:752	The aim of this study was to explore the protective effect of Colomast®, a new preparation containing Adelmidrol, an enhancer of physiological of palmitoylethanolamide (PEA), and sodium hyaluronate in an animal model of immobilization stress.
33143356	4	22	contain	containing	671:680	arg1	Colomast®					642:650	Colomast®	642:650	Colomast®	642:650	The aim of this study was to explore the protective effect of Colomast®, a new preparation containing Adelmidrol, an enhancer of physiological of palmitoylethanolamide (PEA), and sodium hyaluronate in an animal model of immobilization stress.
33143356	4	22	contain	containing	671:680	arg2	Adelmidrol					682:691	Adelmidrol	682:691	Adelmidrol	682:691	The aim of this study was to explore the protective effect of Colomast®, a new preparation containing Adelmidrol, an enhancer of physiological of palmitoylethanolamide (PEA), and sodium hyaluronate in an animal model of immobilization stress.
33143356	4	23	theme	animal	784:789	arg1	model					791:795	an animal model	781:795	an animal model of immobilization stress	781:820	The aim of this study was to explore the protective effect of Colomast®, a new preparation containing Adelmidrol, an enhancer of physiological of palmitoylethanolamide (PEA), and sodium hyaluronate in an animal model of immobilization stress.
33143356	9	24	theme	potential	1637:1645	arg1	approach					1647:1654	a potential approach	1635:1654	a potential approach to ARS	1635:1661	In conclusion, our results suggest Colomast® to be a potential approach to ARS.
33143356	1	25	from	threats	212:218	arg1	balance					247:253	the homeostatic balance	231:253	the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones	231:412	Stress is generally defined as a homeostatic disruption from actual or implied threats and alters the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones.
33143356	1	25	from	threats	212:218	arg1	disruption					178:187	a homeostatic disruption	164:187	a homeostatic disruption from actual or implied threats	164:218	Stress is generally defined as a homeostatic disruption from actual or implied threats and alters the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones.
33143356	1	25	from	threats	212:218	arg1	Stress					133:138	Stress	133:138	Stress	133:138	Stress is generally defined as a homeostatic disruption from actual or implied threats and alters the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones.
33143356	0	26	theme	Protective	0:9	arg1	effects					11:17	Protective effects	0:17	Protective effects of Colomast®, A New Formulation of Adelmidrol and Sodium Hyaluronate, in A Mouse Model of Acute Restraint Stress.	0:131	Protective effects of Colomast®, A New Formulation of Adelmidrol and Sodium Hyaluronate, in A Mouse Model of Acute Restraint Stress.
33143356	0	27	theme	Colomast®	22:30	arg1	effects					11:17	Protective effects	0:17	Protective effects of Colomast®, A New Formulation of Adelmidrol and Sodium Hyaluronate, in A Mouse Model of Acute Restraint Stress.	0:131	Protective effects of Colomast®, A New Formulation of Adelmidrol and Sodium Hyaluronate, in A Mouse Model of Acute Restraint Stress.
33143356	6	28	theme	nuclear	1238:1244	arg1	NF-kB					1253:1257	nuclear factor NF-kB	1238:1257	nuclear factor NF-kB	1238:1257	Colomast® pre-treatment was able to decrease histopathological changes in the gastrointestinal tract, cytokines expression, neutrophil infiltration, mast cell activation, oxidative stress, as well as modulate nuclear factor NF-kB and apoptosis pathways after ARS induction.
33143356	0	29	dep	Adelmidrol	54:63	arg1	Hyaluronate					76:86	Hyaluronate	76:86	Hyaluronate	76:86	Protective effects of Colomast®, A New Formulation of Adelmidrol and Sodium Hyaluronate, in A Mouse Model of Acute Restraint Stress.
33143356	1	30	theme	different	258:266	arg1	axis					354:357	the hypothalamic-pituitary-adrenal axis	319:357	the hypothalamic-pituitary-adrenal axis (HPA)	319:363	Stress is generally defined as a homeostatic disruption from actual or implied threats and alters the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones.
33143356	1	30	theme	different	258:266	arg1	function					306:313	gastrointestinal function	289:313	gastrointestinal function	289:313	Stress is generally defined as a homeostatic disruption from actual or implied threats and alters the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones.
33143356	1	30	theme	different	258:266	arg1	organs					273:278	different body organs	258:278	different body organs	258:278	Stress is generally defined as a homeostatic disruption from actual or implied threats and alters the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones.
33143356	0	31	theme	Mouse	94:98	arg1	Model					100:104	A Mouse Model	92:104	A Mouse Model of Acute Restraint Stress	92:130	Protective effects of Colomast®, A New Formulation of Adelmidrol and Sodium Hyaluronate, in A Mouse Model of Acute Restraint Stress.
33143356	8	32	theme	anxiety-related	1472:1486	arg1	behaviours					1488:1497	anxiety-related behaviours	1472:1497	anxiety-related behaviours	1472:1497	Additionally, we demonstrated that Colomast® ameliorated depression and anxiety-related behaviours, and modulate inflammatory and apoptosis pathways also in brain after ARS induction.
33143356	1	33	theme	homeostatic	166:176	arg1	balance					247:253	the homeostatic balance	231:253	the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones	231:412	Stress is generally defined as a homeostatic disruption from actual or implied threats and alters the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones.
33143356	1	33	theme	homeostatic	166:176	arg1	disruption					178:187	a homeostatic disruption	164:187	a homeostatic disruption from actual or implied threats	164:218	Stress is generally defined as a homeostatic disruption from actual or implied threats and alters the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones.
33143356	1	33	theme	homeostatic	166:176	arg1	Stress					133:138	Stress	133:138	Stress	133:138	Stress is generally defined as a homeostatic disruption from actual or implied threats and alters the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones.
33143356	1	34	theme	body	268:271	arg1	axis					354:357	the hypothalamic-pituitary-adrenal axis	319:357	the hypothalamic-pituitary-adrenal axis (HPA)	319:363	Stress is generally defined as a homeostatic disruption from actual or implied threats and alters the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones.
33143356	1	34	theme	body	268:271	arg1	function					306:313	gastrointestinal function	289:313	gastrointestinal function	289:313	Stress is generally defined as a homeostatic disruption from actual or implied threats and alters the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones.
33143356	1	34	theme	body	268:271	arg1	organs					273:278	different body organs	258:278	different body organs	258:278	Stress is generally defined as a homeostatic disruption from actual or implied threats and alters the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones.
33143356	0	35	theme	Acute	109:113	arg1	Stress					125:130	Acute Restraint Stress	109:130	Acute Restraint Stress	109:130	Protective effects of Colomast®, A New Formulation of Adelmidrol and Sodium Hyaluronate, in A Mouse Model of Acute Restraint Stress.
33143356	1	36	theme	organs	273:278	arg1	balance					247:253	the homeostatic balance	231:253	the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones	231:412	Stress is generally defined as a homeostatic disruption from actual or implied threats and alters the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones.
33143356	1	36	theme	organs	273:278	arg1	disruption					178:187	a homeostatic disruption	164:187	a homeostatic disruption from actual or implied threats	164:218	Stress is generally defined as a homeostatic disruption from actual or implied threats and alters the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones.
33143356	1	36	theme	organs	273:278	arg1	Stress					133:138	Stress	133:138	Stress	133:138	Stress is generally defined as a homeostatic disruption from actual or implied threats and alters the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones.
33143356	2	37	theme	anxiety	494:500	arg1	development					464:474	the development	460:474	the development of depression and anxiety	460:500	Stress is also known to be a risk factor for the development of depression and anxiety.
33143356	6	38	theme	cytokines	1131:1139	arg1	changes					1092:1098	histopathological changes	1074:1098	histopathological changes in the gastrointestinal tract	1074:1128	Colomast® pre-treatment was able to decrease histopathological changes in the gastrointestinal tract, cytokines expression, neutrophil infiltration, mast cell activation, oxidative stress, as well as modulate nuclear factor NF-kB and apoptosis pathways after ARS induction.
33143356	6	38	theme	cytokines	1131:1139	arg1	expression					1141:1150	cytokines expression	1131:1150	cytokines expression	1131:1150	Colomast® pre-treatment was able to decrease histopathological changes in the gastrointestinal tract, cytokines expression, neutrophil infiltration, mast cell activation, oxidative stress, as well as modulate nuclear factor NF-kB and apoptosis pathways after ARS induction.
33143356	5	39	with	fixation	875:882	arg1	tape					933:936	an adhesive tape	921:936	an adhesive tape	921:936	Acute restraint stress (ARS) was induced in mice by fixation for 2 h of the four extremities with an adhesive tape and Colomast® (20 mg/kg) was administered by oral gavage 30 min before the immobilization.
33143356	0	40	theme	Stress	125:130	arg1	Model					100:104	A Mouse Model	92:104	A Mouse Model of Acute Restraint Stress	92:130	Protective effects of Colomast®, A New Formulation of Adelmidrol and Sodium Hyaluronate, in A Mouse Model of Acute Restraint Stress.
33143356	4	41	theme	sodium	759:764	arg1	hyaluronate					766:776	sodium hyaluronate	759:776	sodium hyaluronate	759:776	The aim of this study was to explore the protective effect of Colomast®, a new preparation containing Adelmidrol, an enhancer of physiological of palmitoylethanolamide (PEA), and sodium hyaluronate in an animal model of immobilization stress.
33143356	0	42	theme	New	35:37	arg1	Colomast®					22:30	Colomast®	22:30	Colomast®	22:30	Protective effects of Colomast®, A New Formulation of Adelmidrol and Sodium Hyaluronate, in A Mouse Model of Acute Restraint Stress.
33143356	0	42	theme	New	35:37	arg1	Formulation					39:49	A New Formulation	33:49	A New Formulation of Adelmidrol and Sodium Hyaluronate	33:86	Protective effects of Colomast®, A New Formulation of Adelmidrol and Sodium Hyaluronate, in A Mouse Model of Acute Restraint Stress.
33143356	0	43	theme	Restraint	115:123	arg1	Stress					125:130	Acute Restraint Stress	109:130	Acute Restraint Stress	109:130	Protective effects of Colomast®, A New Formulation of Adelmidrol and Sodium Hyaluronate, in A Mouse Model of Acute Restraint Stress.
33143356	3	44	theme	suitable	537:544	arg1	therapies					546:554	no suitable therapies	534:554	no suitable therapies for treating of stress	534:577	However, until today there are no suitable therapies for treating of stress.
33143356	4	45	theme	Colomast®	642:650	arg1	effect					632:637	the protective effect	617:637	the protective effect of Colomast®, a new preparation containing Adelmidrol, an enhancer of physiological of palmitoylethanolamide (PEA), and sodium hyaluronate in an animal model of immobilization stress	617:820	The aim of this study was to explore the protective effect of Colomast®, a new preparation containing Adelmidrol, an enhancer of physiological of palmitoylethanolamide (PEA), and sodium hyaluronate in an animal model of immobilization stress.
33143356	6	46	theme	gastrointestinal	1107:1122	arg1	tract					1124:1128	the gastrointestinal tract	1103:1128	the gastrointestinal tract	1103:1128	Colomast® pre-treatment was able to decrease histopathological changes in the gastrointestinal tract, cytokines expression, neutrophil infiltration, mast cell activation, oxidative stress, as well as modulate nuclear factor NF-kB and apoptosis pathways after ARS induction.
33143356	4	47	theme	palmitoylethanolamide	726:746	arg1	Adelmidrol					682:691	Adelmidrol	682:691	Adelmidrol	682:691	The aim of this study was to explore the protective effect of Colomast®, a new preparation containing Adelmidrol, an enhancer of physiological of palmitoylethanolamide (PEA), and sodium hyaluronate in an animal model of immobilization stress.
33143356	4	47	theme	palmitoylethanolamide	726:746	arg1	enhancer					697:704	an enhancer	694:704	an enhancer of physiological of palmitoylethanolamide (PEA)	694:752	The aim of this study was to explore the protective effect of Colomast®, a new preparation containing Adelmidrol, an enhancer of physiological of palmitoylethanolamide (PEA), and sodium hyaluronate in an animal model of immobilization stress.
33143356	4	47	theme	palmitoylethanolamide	726:746	arg1	hyaluronate					766:776	sodium hyaluronate	759:776	sodium hyaluronate	759:776	The aim of this study was to explore the protective effect of Colomast®, a new preparation containing Adelmidrol, an enhancer of physiological of palmitoylethanolamide (PEA), and sodium hyaluronate in an animal model of immobilization stress.
33143356	4	48	theme	new	655:657	arg1	preparation					659:669	a new preparation	653:669	a new preparation containing Adelmidrol, an enhancer of physiological of palmitoylethanolamide (PEA), and sodium hyaluronate in an animal model of immobilization stress	653:820	The aim of this study was to explore the protective effect of Colomast®, a new preparation containing Adelmidrol, an enhancer of physiological of palmitoylethanolamide (PEA), and sodium hyaluronate in an animal model of immobilization stress.
33143356	4	48	theme	new	655:657	arg1	Colomast®					642:650	Colomast®	642:650	Colomast®	642:650	The aim of this study was to explore the protective effect of Colomast®, a new preparation containing Adelmidrol, an enhancer of physiological of palmitoylethanolamide (PEA), and sodium hyaluronate in an animal model of immobilization stress.
33143356	6	49	theme	Colomast®	1029:1037	arg1	pre-treatment					1039:1051	Colomast® pre-treatment	1029:1051	Colomast® pre-treatment	1029:1051	Colomast® pre-treatment was able to decrease histopathological changes in the gastrointestinal tract, cytokines expression, neutrophil infiltration, mast cell activation, oxidative stress, as well as modulate nuclear factor NF-kB and apoptosis pathways after ARS induction.
33143356	1	50	theme	actual	194:199	arg1	threats					212:218	actual or implied threats	194:218	actual or implied threats	194:218	Stress is generally defined as a homeostatic disruption from actual or implied threats and alters the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones.
33143356	6	51	theme	oxidative	1200:1208	arg1	changes					1092:1098	histopathological changes	1074:1098	histopathological changes in the gastrointestinal tract	1074:1128	Colomast® pre-treatment was able to decrease histopathological changes in the gastrointestinal tract, cytokines expression, neutrophil infiltration, mast cell activation, oxidative stress, as well as modulate nuclear factor NF-kB and apoptosis pathways after ARS induction.
33143356	6	51	theme	oxidative	1200:1208	arg1	stress					1210:1215	oxidative stress	1200:1215	oxidative stress	1200:1215	Colomast® pre-treatment was able to decrease histopathological changes in the gastrointestinal tract, cytokines expression, neutrophil infiltration, mast cell activation, oxidative stress, as well as modulate nuclear factor NF-kB and apoptosis pathways after ARS induction.
33143356	1	52	theme	glucocorticoid	390:403	arg1	hormones					405:412	glucocorticoid hormones	390:412	glucocorticoid hormones	390:412	Stress is generally defined as a homeostatic disruption from actual or implied threats and alters the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones.
33143356	8	53	theme	inflammatory	1513:1524	arg1	pathways					1540:1547	inflammatory and apoptosis pathways	1513:1547	pathways	1540:1547	Additionally, we demonstrated that Colomast® ameliorated depression and anxiety-related behaviours, and modulate inflammatory and apoptosis pathways also in brain after ARS induction.
33143356	0	54	theme	Adelmidrol	54:63	arg1	Colomast®					22:30	Colomast®	22:30	Colomast®	22:30	Protective effects of Colomast®, A New Formulation of Adelmidrol and Sodium Hyaluronate, in A Mouse Model of Acute Restraint Stress.
33143356	0	54	theme	Adelmidrol	54:63	arg1	Formulation					39:49	A New Formulation	33:49	A New Formulation of Adelmidrol and Sodium Hyaluronate	33:86	Protective effects of Colomast®, A New Formulation of Adelmidrol and Sodium Hyaluronate, in A Mouse Model of Acute Restraint Stress.
33143356	1	55	theme	hormones	405:412	arg1	release					379:385	the release	375:385	the release of glucocorticoid hormones	375:412	Stress is generally defined as a homeostatic disruption from actual or implied threats and alters the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones.
33143356	4	56	theme	protective	621:630	arg1	effect					632:637	the protective effect	617:637	the protective effect of Colomast®, a new preparation containing Adelmidrol, an enhancer of physiological of palmitoylethanolamide (PEA), and sodium hyaluronate in an animal model of immobilization stress	617:820	The aim of this study was to explore the protective effect of Colomast®, a new preparation containing Adelmidrol, an enhancer of physiological of palmitoylethanolamide (PEA), and sodium hyaluronate in an animal model of immobilization stress.
33143356	8	57	theme	ARS	1569:1571	arg1	induction					1573:1581	ARS induction	1569:1581	ARS induction	1569:1581	Additionally, we demonstrated that Colomast® ameliorated depression and anxiety-related behaviours, and modulate inflammatory and apoptosis pathways also in brain after ARS induction.
33143356	1	58	theme	implied	204:210	arg1	threats					212:218	actual or implied threats	194:218	actual or implied threats	194:218	Stress is generally defined as a homeostatic disruption from actual or implied threats and alters the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones.
33143356	6	59	theme	ARS	1288:1290	arg1	induction					1292:1300	ARS induction	1288:1300	ARS induction	1288:1300	Colomast® pre-treatment was able to decrease histopathological changes in the gastrointestinal tract, cytokines expression, neutrophil infiltration, mast cell activation, oxidative stress, as well as modulate nuclear factor NF-kB and apoptosis pathways after ARS induction.
33143356	1	60	theme	gastrointestinal	289:304	arg1	function					306:313	gastrointestinal function	289:313	gastrointestinal function	289:313	Stress is generally defined as a homeostatic disruption from actual or implied threats and alters the homeostatic balance of different body organs, such as gastrointestinal function and the hypothalamic-pituitary-adrenal axis (HPA), inducing the release of glucocorticoid hormones.
33143356	5	61	theme	extremities	904:914	arg1	h					890:890	2 h	888:890	2 h of the four extremities	888:914	Acute restraint stress (ARS) was induced in mice by fixation for 2 h of the four extremities with an adhesive tape and Colomast® (20 mg/kg) was administered by oral gavage 30 min before the immobilization.
33143356	5	62	theme	Acute	823:827	arg1	ARS					847:849	ARS	847:849	ARS	847:849	Acute restraint stress (ARS) was induced in mice by fixation for 2 h of the four extremities with an adhesive tape and Colomast® (20 mg/kg) was administered by oral gavage 30 min before the immobilization.
33143356	5	62	theme	Acute	823:827	arg1	stress					839:844	Acute restraint stress	823:844	Acute restraint stress (ARS)	823:850	Acute restraint stress (ARS) was induced in mice by fixation for 2 h of the four extremities with an adhesive tape and Colomast® (20 mg/kg) was administered by oral gavage 30 min before the immobilization.
33143356	6	63	theme	cell	1183:1186	arg1	changes					1092:1098	histopathological changes	1074:1098	histopathological changes in the gastrointestinal tract	1074:1128	Colomast® pre-treatment was able to decrease histopathological changes in the gastrointestinal tract, cytokines expression, neutrophil infiltration, mast cell activation, oxidative stress, as well as modulate nuclear factor NF-kB and apoptosis pathways after ARS induction.
33143356	6	63	theme	cell	1183:1186	arg1	activation					1188:1197	mast cell activation	1178:1197	mast cell activation	1178:1197	Colomast® pre-treatment was able to decrease histopathological changes in the gastrointestinal tract, cytokines expression, neutrophil infiltration, mast cell activation, oxidative stress, as well as modulate nuclear factor NF-kB and apoptosis pathways after ARS induction.
33143356	8	64	theme	apoptosis	1530:1538	arg1	pathways					1540:1547	inflammatory and apoptosis pathways	1513:1547	pathways	1540:1547	Additionally, we demonstrated that Colomast® ameliorated depression and anxiety-related behaviours, and modulate inflammatory and apoptosis pathways also in brain after ARS induction.
33577603	5	0	theme	ILY-mediated	738:749	arg1	lysis					756:760	ILY-mediated cell lysis	738:760	ILY-mediated cell lysis	738:760	In this study, we applied genome-wide CRISPR-Cas9 knock-out screening to reveal additional cellular co-factors essential for ILY-mediated cell lysis.
33577603	5	1	theme	cell	751:754	arg1	lysis					756:760	ILY-mediated cell lysis	738:760	ILY-mediated cell lysis	738:760	In this study, we applied genome-wide CRISPR-Cas9 knock-out screening to reveal additional cellular co-factors essential for ILY-mediated cell lysis.
33577603	9	2	theme	cytolytic	1272:1280	arg1	activity					1282:1289	pneumolysin's cytolytic activity	1258:1289	pneumolysin's cytolytic activity	1258:1289	We also cross-validated identified genes with vaginolysin and pneumolysin and found that pneumolysin's cytolytic activity strongly depends on the asymmetric distribution of membrane phospholipids.
33577603	6	3	theme	genes	791:795	arg1	plethora					779:786	a plethora	777:786	a plethora of genes previously not associated with ILY, many of which are important for membrane constitution	777:885	We discovered a plethora of genes previously not associated with ILY, many of which are important for membrane constitution.
33577603	6	3	theme	genes	791:795	arg1	many					833:836	many	833:836	many	833:836	We discovered a plethora of genes previously not associated with ILY, many of which are important for membrane constitution.
33577603	6	3	theme	genes	791:795	arg1	genes					791:795	genes	791:795	genes	791:795	We discovered a plethora of genes previously not associated with ILY, many of which are important for membrane constitution.
33577603	1	4	theme	large	233:237	arg1	variety					239:245	a large variety	231:245	a large variety of pathogenic bacteria	231:268	Cholesterol-dependent cytolysins (CDCs), of which intermedilysin (ILY) is an archetypal member, are a group of pore-forming toxins secreted by a large variety of pathogenic bacteria.
33577603	1	4	theme	large	233:237	arg1	bacteria					261:268	pathogenic bacteria	250:268	pathogenic bacteria	250:268	Cholesterol-dependent cytolysins (CDCs), of which intermedilysin (ILY) is an archetypal member, are a group of pore-forming toxins secreted by a large variety of pathogenic bacteria.
33577603	6	5	theme	membrane	865:872	arg1	constitution					874:885	membrane constitution	865:885	membrane constitution	865:885	We discovered a plethora of genes previously not associated with ILY, many of which are important for membrane constitution.
33577603	10	6	theme	membrane-targeting	1388:1405	arg1	toxins					1407:1412	membrane-targeting toxins	1388:1412	membrane-targeting toxins combined with genetic screening	1388:1444	This study shows that membrane-targeting toxins combined with genetic screening can identify genes involved in biological membrane composition and metabolism.
33577603	5	7	theme	additional	693:702	arg1	co-factors					713:722	additional cellular co-factors	693:722	additional cellular co-factors essential for ILY-mediated cell lysis	693:760	In this study, we applied genome-wide CRISPR-Cas9 knock-out screening to reveal additional cellular co-factors essential for ILY-mediated cell lysis.
33577603	5	8	theme	knock-out	663:671	arg1	screening					673:681	genome-wide CRISPR-Cas9 knock-out screening	639:681	genome-wide CRISPR-Cas9 knock-out screening	639:681	In this study, we applied genome-wide CRISPR-Cas9 knock-out screening to reveal additional cellular co-factors essential for ILY-mediated cell lysis.
33577603	9	9	theme	asymmetric	1315:1324	arg1	distribution					1326:1337	the asymmetric distribution	1311:1337	the asymmetric distribution of membrane phospholipids	1311:1363	We also cross-validated identified genes with vaginolysin and pneumolysin and found that pneumolysin's cytolytic activity strongly depends on the asymmetric distribution of membrane phospholipids.
33577603	3	10	theme	cell	494:497	arg1	lysis					499:503	cell lysis	494:503	cell lysis	494:503	These pores disrupt cell membranes and result in cell lysis.
33577603	8	11	theme	ILY	1155:1157	arg1	activity					1159:1166	ILY activity	1155:1166	ILY activity	1155:1166	Furthermore, we identified hits in both protein and lipid glycosylation pathways and show a role for glucosylceramide, demonstrating that membrane organization is important for ILY activity.
33577603	5	12	theme	cellular	704:711	arg1	co-factors					713:722	additional cellular co-factors	693:722	additional cellular co-factors essential for ILY-mediated cell lysis	693:760	In this study, we applied genome-wide CRISPR-Cas9 knock-out screening to reveal additional cellular co-factors essential for ILY-mediated cell lysis.
33577603	10	13	theme	membrane	1488:1495	arg1	composition					1497:1507	biological membrane composition	1477:1507	biological membrane composition	1477:1507	This study shows that membrane-targeting toxins combined with genetic screening can identify genes involved in biological membrane composition and metabolism.
33577603	2	14	from	interaction	332:342	arg1	membrane					375:382	the target membrane	364:382	the target membrane	364:382	These toxins, secreted as soluble monomers, oligomerize upon interaction with cholesterol in the target membrane and transect it as pores of diameters of up to 100 to 300 Å.
33577603	1	15	theme	Cholesterol-dependent	88:108	arg1	group					190:194	a group	188:194	a group of pore-forming toxins secreted by a large variety of pathogenic bacteria	188:268	Cholesterol-dependent cytolysins (CDCs), of which intermedilysin (ILY) is an archetypal member, are a group of pore-forming toxins secreted by a large variety of pathogenic bacteria.
33577603	1	15	theme	Cholesterol-dependent	88:108	arg1	CDCs					122:125	CDCs	122:125	CDCs	122:125	Cholesterol-dependent cytolysins (CDCs), of which intermedilysin (ILY) is an archetypal member, are a group of pore-forming toxins secreted by a large variety of pathogenic bacteria.
33577603	1	15	theme	Cholesterol-dependent	88:108	arg1	toxins					212:217	pore-forming toxins	199:217	pore-forming toxins secreted by a large variety of pathogenic bacteria	199:268	Cholesterol-dependent cytolysins (CDCs), of which intermedilysin (ILY) is an archetypal member, are a group of pore-forming toxins secreted by a large variety of pathogenic bacteria.
33577603	1	15	theme	Cholesterol-dependent	88:108	arg1	cytolysins					110:119	Cholesterol-dependent cytolysins	88:119	Cholesterol-dependent cytolysins (CDCs)	88:126	Cholesterol-dependent cytolysins (CDCs), of which intermedilysin (ILY) is an archetypal member, are a group of pore-forming toxins secreted by a large variety of pathogenic bacteria.
33577603	2	16	theme	target	368:373	arg1	membrane					375:382	the target membrane	364:382	the target membrane	364:382	These toxins, secreted as soluble monomers, oligomerize upon interaction with cholesterol in the target membrane and transect it as pores of diameters of up to 100 to 300 Å.
33577603	0	17	theme	cytolytic	15:23	arg1	activity					25:32	Intermedilysin cytolytic activity	0:32	Intermedilysin cytolytic activity	0:32	Intermedilysin cytolytic activity depends on heparan sulfates and membrane composition.
33577603	10	18	theme	biological	1477:1486	arg1	composition					1497:1507	biological membrane composition	1477:1507	biological membrane composition	1477:1507	This study shows that membrane-targeting toxins combined with genetic screening can identify genes involved in biological membrane composition and metabolism.
33577603	8	19	from	hits	1005:1008	arg1	pathways					1050:1057	both protein and lipid glycosylation pathways	1013:1057	pathways	1050:1057	Furthermore, we identified hits in both protein and lipid glycosylation pathways and show a role for glucosylceramide, demonstrating that membrane organization is important for ILY activity.
33577603	8	19	from	hits	1005:1008	arg1	protein					1018:1024	both protein and lipid glycosylation pathways	1013:1057	protein	1018:1024	Furthermore, we identified hits in both protein and lipid glycosylation pathways and show a role for glucosylceramide, demonstrating that membrane organization is important for ILY activity.
33577603	1	20	theme	cytolysins	110:119	arg1	member					176:181	an archetypal member	162:181	an archetypal member	162:181	Cholesterol-dependent cytolysins (CDCs), of which intermedilysin (ILY) is an archetypal member, are a group of pore-forming toxins secreted by a large variety of pathogenic bacteria.
33577603	1	20	theme	cytolysins	110:119	arg1	intermedilysin					138:151	intermedilysin	138:151	intermedilysin (ILY)	138:157	Cholesterol-dependent cytolysins (CDCs), of which intermedilysin (ILY) is an archetypal member, are a group of pore-forming toxins secreted by a large variety of pathogenic bacteria.
33577603	8	21	theme	glycosylation	1036:1048	arg1	pathways					1050:1057	both protein and lipid glycosylation pathways	1013:1057	pathways	1050:1057	Furthermore, we identified hits in both protein and lipid glycosylation pathways and show a role for glucosylceramide, demonstrating that membrane organization is important for ILY activity.
33577603	0	22	theme	Intermedilysin	0:13	arg1	activity					25:32	Intermedilysin cytolytic activity	0:32	Intermedilysin cytolytic activity	0:32	Intermedilysin cytolytic activity depends on heparan sulfates and membrane composition.
33577603	1	23	theme	pathogenic	250:259	arg1	bacteria					261:268	pathogenic bacteria	250:268	pathogenic bacteria	250:268	Cholesterol-dependent cytolysins (CDCs), of which intermedilysin (ILY) is an archetypal member, are a group of pore-forming toxins secreted by a large variety of pathogenic bacteria.
33577603	4	24	theme	well-established	536:551	arg1	factor					562:567	a well-established cellular factor	534:567	a well-established cellular factor required for intermedilysin pore formation	534:610	The immune receptor CD59 is a well-established cellular factor required for intermedilysin pore formation.
33577603	4	24	theme	well-established	536:551	arg1	CD59					526:529	The immune receptor CD59	506:529	The immune receptor CD59	506:529	The immune receptor CD59 is a well-established cellular factor required for intermedilysin pore formation.
33577603	1	25	theme	bacteria	261:268	arg1	variety					239:245	a large variety	231:245	a large variety of pathogenic bacteria	231:268	Cholesterol-dependent cytolysins (CDCs), of which intermedilysin (ILY) is an archetypal member, are a group of pore-forming toxins secreted by a large variety of pathogenic bacteria.
33577603	1	25	theme	bacteria	261:268	arg1	bacteria					261:268	pathogenic bacteria	250:268	pathogenic bacteria	250:268	Cholesterol-dependent cytolysins (CDCs), of which intermedilysin (ILY) is an archetypal member, are a group of pore-forming toxins secreted by a large variety of pathogenic bacteria.
33577603	5	26	theme	CRISPR-Cas9	651:661	arg1	screening					673:681	genome-wide CRISPR-Cas9 knock-out screening	639:681	genome-wide CRISPR-Cas9 knock-out screening	639:681	In this study, we applied genome-wide CRISPR-Cas9 knock-out screening to reveal additional cellular co-factors essential for ILY-mediated cell lysis.
33577603	9	27	theme	identified	1193:1202	arg1	genes					1204:1208	identified genes	1193:1208	identified genes	1193:1208	We also cross-validated identified genes with vaginolysin and pneumolysin and found that pneumolysin's cytolytic activity strongly depends on the asymmetric distribution of membrane phospholipids.
33577603	4	28	theme	intermedilysin	582:595	arg1	formation					602:610	intermedilysin pore formation	582:610	intermedilysin pore formation	582:610	The immune receptor CD59 is a well-established cellular factor required for intermedilysin pore formation.
33577603	4	29	theme	immune	510:515	arg1	factor					562:567	a well-established cellular factor	534:567	a well-established cellular factor required for intermedilysin pore formation	534:610	The immune receptor CD59 is a well-established cellular factor required for intermedilysin pore formation.
33577603	4	29	theme	immune	510:515	arg1	CD59					526:529	The immune receptor CD59	506:529	The immune receptor CD59	506:529	The immune receptor CD59 is a well-established cellular factor required for intermedilysin pore formation.
33577603	2	30	theme	diameters	412:420	arg1	pores					403:407	pores	403:407	pores of diameters of up to 100 to 300 Å	403:442	These toxins, secreted as soluble monomers, oligomerize upon interaction with cholesterol in the target membrane and transect it as pores of diameters of up to 100 to 300 Å.
33577603	2	30	theme	diameters	412:420	arg1	it					397:398	it	397:398	it	397:398	These toxins, secreted as soluble monomers, oligomerize upon interaction with cholesterol in the target membrane and transect it as pores of diameters of up to 100 to 300 Å.
33577603	10	31	theme	genetic	1428:1434	arg1	screening					1436:1444	genetic screening	1428:1444	genetic screening	1428:1444	This study shows that membrane-targeting toxins combined with genetic screening can identify genes involved in biological membrane composition and metabolism.
33577603	0	32	theme	heparan	45:51	arg1	sulfates					53:60	heparan sulfates	45:60	heparan sulfates	45:60	Intermedilysin cytolytic activity depends on heparan sulfates and membrane composition.
33577603	9	33	theme	membrane	1342:1349	arg1	phospholipids					1351:1363	membrane phospholipids	1342:1363	membrane phospholipids	1342:1363	We also cross-validated identified genes with vaginolysin and pneumolysin and found that pneumolysin's cytolytic activity strongly depends on the asymmetric distribution of membrane phospholipids.
33577603	8	34	theme	lipid	1030:1034	arg1	pathways					1050:1057	both protein and lipid glycosylation pathways	1013:1057	pathways	1050:1057	Furthermore, we identified hits in both protein and lipid glycosylation pathways and show a role for glucosylceramide, demonstrating that membrane organization is important for ILY activity.
33577603	9	35	theme	phospholipids	1351:1363	arg1	distribution					1326:1337	the asymmetric distribution	1311:1337	the asymmetric distribution of membrane phospholipids	1311:1363	We also cross-validated identified genes with vaginolysin and pneumolysin and found that pneumolysin's cytolytic activity strongly depends on the asymmetric distribution of membrane phospholipids.
33577603	4	36	theme	receptor	517:524	arg1	factor					562:567	a well-established cellular factor	534:567	a well-established cellular factor required for intermedilysin pore formation	534:610	The immune receptor CD59 is a well-established cellular factor required for intermedilysin pore formation.
33577603	4	36	theme	receptor	517:524	arg1	CD59					526:529	The immune receptor CD59	506:529	The immune receptor CD59	506:529	The immune receptor CD59 is a well-established cellular factor required for intermedilysin pore formation.
33577603	4	37	theme	pore	597:600	arg1	formation					602:610	intermedilysin pore formation	582:610	intermedilysin pore formation	582:610	The immune receptor CD59 is a well-established cellular factor required for intermedilysin pore formation.
33577603	2	38	theme	up	425:426	arg1	diameters					412:420	diameters	412:420	diameters of up to 100 to 300 Å	412:442	These toxins, secreted as soluble monomers, oligomerize upon interaction with cholesterol in the target membrane and transect it as pores of diameters of up to 100 to 300 Å.
33577603	2	39	dep	300	438:440	arg1	to					435:436	to	435:436	to	435:436	These toxins, secreted as soluble monomers, oligomerize upon interaction with cholesterol in the target membrane and transect it as pores of diameters of up to 100 to 300 Å.
33577603	2	40	with	interaction	332:342	arg1	cholesterol					349:359	cholesterol	349:359	cholesterol	349:359	These toxins, secreted as soluble monomers, oligomerize upon interaction with cholesterol in the target membrane and transect it as pores of diameters of up to 100 to 300 Å.
33577603	7	41	theme	heparan	901:907	arg1	sulfates					909:916	heparan sulfates	901:916	heparan sulfates	901:916	We show that heparan sulfates facilitate ILY activity, which can be inhibited by heparin.
33577603	1	42	theme	pore-forming	199:210	arg1	toxins					212:217	pore-forming toxins	199:217	pore-forming toxins secreted by a large variety of pathogenic bacteria	199:268	Cholesterol-dependent cytolysins (CDCs), of which intermedilysin (ILY) is an archetypal member, are a group of pore-forming toxins secreted by a large variety of pathogenic bacteria.
33577603	5	43	theme	genome-wide	639:649	arg1	screening					673:681	genome-wide CRISPR-Cas9 knock-out screening	639:681	genome-wide CRISPR-Cas9 knock-out screening	639:681	In this study, we applied genome-wide CRISPR-Cas9 knock-out screening to reveal additional cellular co-factors essential for ILY-mediated cell lysis.
33577603	2	44	theme	soluble	297:303	arg1	monomers					305:312	soluble monomers	297:312	soluble monomers	297:312	These toxins, secreted as soluble monomers, oligomerize upon interaction with cholesterol in the target membrane and transect it as pores of diameters of up to 100 to 300 Å.
33577603	1	45	theme	toxins	212:217	arg1	group					190:194	a group	188:194	a group of pore-forming toxins secreted by a large variety of pathogenic bacteria	188:268	Cholesterol-dependent cytolysins (CDCs), of which intermedilysin (ILY) is an archetypal member, are a group of pore-forming toxins secreted by a large variety of pathogenic bacteria.
33577603	1	45	theme	toxins	212:217	arg1	toxins					212:217	pore-forming toxins	199:217	pore-forming toxins secreted by a large variety of pathogenic bacteria	199:268	Cholesterol-dependent cytolysins (CDCs), of which intermedilysin (ILY) is an archetypal member, are a group of pore-forming toxins secreted by a large variety of pathogenic bacteria.
33577603	1	45	theme	toxins	212:217	arg1	cytolysins					110:119	Cholesterol-dependent cytolysins	88:119	Cholesterol-dependent cytolysins (CDCs)	88:126	Cholesterol-dependent cytolysins (CDCs), of which intermedilysin (ILY) is an archetypal member, are a group of pore-forming toxins secreted by a large variety of pathogenic bacteria.
33577603	5	46	theme	essential	724:732	arg1	co-factors					713:722	additional cellular co-factors	693:722	additional cellular co-factors essential for ILY-mediated cell lysis	693:760	In this study, we applied genome-wide CRISPR-Cas9 knock-out screening to reveal additional cellular co-factors essential for ILY-mediated cell lysis.
33577603	3	47	theme	cell	465:468	arg1	membranes					470:478	cell membranes	465:478	cell membranes	465:478	These pores disrupt cell membranes and result in cell lysis.
33577603	1	48	theme	archetypal	165:174	arg1	member					176:181	an archetypal member	162:181	an archetypal member	162:181	Cholesterol-dependent cytolysins (CDCs), of which intermedilysin (ILY) is an archetypal member, are a group of pore-forming toxins secreted by a large variety of pathogenic bacteria.
33577603	1	48	theme	archetypal	165:174	arg1	intermedilysin					138:151	intermedilysin	138:151	intermedilysin (ILY)	138:157	Cholesterol-dependent cytolysins (CDCs), of which intermedilysin (ILY) is an archetypal member, are a group of pore-forming toxins secreted by a large variety of pathogenic bacteria.
33577603	0	49	theme	membrane	66:73	arg1	composition					75:85	membrane composition	66:85	membrane composition	66:85	Intermedilysin cytolytic activity depends on heparan sulfates and membrane composition.
33577603	4	50	theme	cellular	553:560	arg1	factor					562:567	a well-established cellular factor	534:567	a well-established cellular factor required for intermedilysin pore formation	534:610	The immune receptor CD59 is a well-established cellular factor required for intermedilysin pore formation.
33577603	4	50	theme	cellular	553:560	arg1	CD59					526:529	The immune receptor CD59	506:529	The immune receptor CD59	506:529	The immune receptor CD59 is a well-established cellular factor required for intermedilysin pore formation.
33577603	8	51	theme	membrane	1116:1123	arg1	organization					1125:1136	membrane organization	1116:1136	membrane organization	1116:1136	Furthermore, we identified hits in both protein and lipid glycosylation pathways and show a role for glucosylceramide, demonstrating that membrane organization is important for ILY activity.
31894888	10	0	theme	future	1916:1921	arg1	candidate					1923:1931	a future candidate	1914:1931	a future candidate for promoting new tissues artificially	1914:1970	These results indicate that fabricated composite nanofiber scaffolds with multifunctional characteristics will have a highest potential as a future candidate for promoting new tissues artificially.
31894888	10	0	theme	future	1916:1921	arg1	potential					1901:1909	a highest potential	1891:1909	a highest potential	1891:1909	These results indicate that fabricated composite nanofiber scaffolds with multifunctional characteristics will have a highest potential as a future candidate for promoting new tissues artificially.
31894888	9	1	theme	fabricated	1631:1640	arg1	scaffolds					1642:1650	the fabricated scaffolds	1627:1650	the fabricated scaffolds	1627:1650	Furthermore, the fabricated scaffolds were subjected to analysis in simulated body fluid at 37°C to induce mineralization for future osseous tissue integration.
31894888	7	2	theme	Ag	1385:1386	arg1	NPs					1388:1390	Ag NPs	1385:1390	Ag NPs	1385:1390	The antibacterial efficiency of the nanofibers was scrutinized against Escherichia coli and Staphylococcus aureus, revealing proper in situ deposition of Ag NPs and confirming the nanofibers are antibacterial in nature.
31894888	3	3	theme	cellulose	686:694	arg1	nanofibers					696:705	pure cellulose nanofibers	681:705	pure cellulose nanofibers containing TiO2 NPs	681:725	The fabricated composite scaffolds were dispensed to alkaline deacetylation using 0.05 M NaOH to remove the acetyl groups in order to generate pure cellulose nanofibers containing TiO2 NPs.
31894888	6	4	theme	complete	1193:1200	arg1	deacetylation					1202:1214	complete deacetylation	1193:1214	complete deacetylation of nanofibers	1193:1228	This demonstrated the presence of both TiO2 and Ag NPs and complete deacetylation of nanofibers.
31894888	1	5	theme	novel	193:197	arg1	strategy					199:206	a novel strategy	191:206	a novel strategy	191:206	In the present work, a novel strategy was explored to fabricate nanofiber scaffolds consisting of cellulose assimilated with titanium dioxide (TiO2 ) and silver (Ag) nanoparticles (NPs).
31894888	6	6	theme	NPs	1185:1187	arg1	presence					1156:1163	the presence	1152:1163	the presence of both TiO2 and Ag NPs	1152:1187	This demonstrated the presence of both TiO2 and Ag NPs and complete deacetylation of nanofibers.
31894888	6	6	theme	NPs	1185:1187	arg1	deacetylation					1202:1214	complete deacetylation	1193:1214	complete deacetylation of nanofibers	1193:1228	This demonstrated the presence of both TiO2 and Ag NPs and complete deacetylation of nanofibers.
31894888	3	7	theme	fabricated	542:551	arg1	scaffolds					563:571	The fabricated composite scaffolds	538:571	The fabricated composite scaffolds	538:571	The fabricated composite scaffolds were dispensed to alkaline deacetylation using 0.05 M NaOH to remove the acetyl groups in order to generate pure cellulose nanofibers containing TiO2 NPs.
31894888	9	8	from	analysis	1670:1677	arg1	37°C					1706:1709	37°C	1706:1709	37°C	1706:1709	Furthermore, the fabricated scaffolds were subjected to analysis in simulated body fluid at 37°C to induce mineralization for future osseous tissue integration.
31894888	9	8	from	analysis	1670:1677	arg1	fluid					1697:1701	simulated body fluid	1682:1701	simulated body fluid at 37°C to induce mineralization for future osseous tissue integration	1682:1772	Furthermore, the fabricated scaffolds were subjected to analysis in simulated body fluid at 37°C to induce mineralization for future osseous tissue integration.
31894888	2	9	theme	colloidal	517:525	arg1	solutions					527:535	colloidal solutions	517:535	colloidal solutions	517:535	The concentration of the TiO2 NPs in the composite was adjusted to 1.0, 1.5, and 2.0 wt % with respect to polymer concentration used for the electrospinning of colloidal solutions.
31894888	2	10	from	concentration	361:373	arg1	composite					398:406	the composite	394:406	the composite	394:406	The concentration of the TiO2 NPs in the composite was adjusted to 1.0, 1.5, and 2.0 wt % with respect to polymer concentration used for the electrospinning of colloidal solutions.
31894888	7	11	theme	in	1363:1364	arg1	deposition					1371:1380	proper in situ deposition	1356:1380	proper in situ deposition of Ag NPs	1356:1390	The antibacterial efficiency of the nanofibers was scrutinized against Escherichia coli and Staphylococcus aureus, revealing proper in situ deposition of Ag NPs and confirming the nanofibers are antibacterial in nature.
31894888	0	12	theme	engineering	144:154	arg1	applications					156:167	future tissue engineering applications	130:167	future tissue engineering applications	130:167	Fabrication of multifunctional cellulose/TiO2 /Ag composite nanofibers scaffold with antibacterial and bioactivity properties for future tissue engineering applications.
31894888	5	13	theme	transmission	1027:1038	arg1	TEM					1061:1063	TEM	1061:1063	TEM	1061:1063	The physicochemical properties of the nanofibers were identified by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) and contact angle meter studies.
31894888	5	13	theme	transmission	1027:1038	arg1	microscopy					1049:1058	transmission electron microscopy	1027:1058	transmission electron microscopy (TEM)	1027:1064	The physicochemical properties of the nanofibers were identified by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) and contact angle meter studies.
31894888	4	14	dep	in	806:807	arg1	situ					809:812	situ	809:812	situ	809:812	Moreover, to augment our nanofiber scaffolds with antibacterial activity, the in situ deposition approach of using Ag NPs was utilized with varied molar concentrations of 0.14, 0.42, and 0.71 M.
31894888	9	15	theme	body	1692:1695	arg1	fluid					1697:1701	simulated body fluid	1682:1701	simulated body fluid at 37°C to induce mineralization for future osseous tissue integration	1682:1772	Furthermore, the fabricated scaffolds were subjected to analysis in simulated body fluid at 37°C to induce mineralization for future osseous tissue integration.
31894888	6	16	theme	Ag	1182:1183	arg1	NPs					1185:1187	both TiO2 and Ag NPs	1168:1187	both TiO2 and Ag NPs	1168:1187	This demonstrated the presence of both TiO2 and Ag NPs and complete deacetylation of nanofibers.
31894888	1	17	theme	nanofiber	234:242	arg1	scaffolds					244:252	nanofiber scaffolds	234:252	nanofiber scaffolds consisting of cellulose assimilated with titanium dioxide (TiO2 ) and silver (Ag) nanoparticles (NPs)	234:354	In the present work, a novel strategy was explored to fabricate nanofiber scaffolds consisting of cellulose assimilated with titanium dioxide (TiO2 ) and silver (Ag) nanoparticles (NPs).
31894888	4	18	theme	varied	868:873	arg1	concentrations					881:894	varied molar concentrations	868:894	varied molar concentrations of 0.14, 0.42, and 0.71 M	868:920	Moreover, to augment our nanofiber scaffolds with antibacterial activity, the in situ deposition approach of using Ag NPs was utilized with varied molar concentrations of 0.14, 0.42, and 0.71 M.
31894888	6	19	theme	TiO2	1173:1176	arg1	NPs					1185:1187	both TiO2 and Ag NPs	1168:1187	both TiO2 and Ag NPs	1168:1187	This demonstrated the presence of both TiO2 and Ag NPs and complete deacetylation of nanofibers.
31894888	10	20	with	scaffolds	1834:1842	arg1	characteristics					1865:1879	multifunctional characteristics	1849:1879	multifunctional characteristics	1849:1879	These results indicate that fabricated composite nanofiber scaffolds with multifunctional characteristics will have a highest potential as a future candidate for promoting new tissues artificially.
31894888	10	21	theme	composite	1814:1822	arg1	scaffolds					1834:1842	fabricated composite nanofiber scaffolds	1803:1842	fabricated composite nanofiber scaffolds with multifunctional characteristics	1803:1879	These results indicate that fabricated composite nanofiber scaffolds with multifunctional characteristics will have a highest potential as a future candidate for promoting new tissues artificially.
31894888	1	22	theme	present	177:183	arg1	work					185:188	the present work	173:188	the present work	173:188	In the present work, a novel strategy was explored to fabricate nanofiber scaffolds consisting of cellulose assimilated with titanium dioxide (TiO2 ) and silver (Ag) nanoparticles (NPs).
31894888	9	23	from	37°C	1706:1709	arg1	fluid					1697:1701	simulated body fluid	1682:1701	simulated body fluid at 37°C to induce mineralization for future osseous tissue integration	1682:1772	Furthermore, the fabricated scaffolds were subjected to analysis in simulated body fluid at 37°C to induce mineralization for future osseous tissue integration.
31894888	9	23	from	37°C	1706:1709	arg1	analysis					1670:1677	analysis	1670:1677	analysis in simulated body fluid at 37°C to induce mineralization for future osseous tissue integration	1670:1772	Furthermore, the fabricated scaffolds were subjected to analysis in simulated body fluid at 37°C to induce mineralization for future osseous tissue integration.
31894888	9	24	theme	future	1740:1745	arg1	integration					1762:1772	future osseous tissue integration	1740:1772	future osseous tissue integration	1740:1772	Furthermore, the fabricated scaffolds were subjected to analysis in simulated body fluid at 37°C to induce mineralization for future osseous tissue integration.
31894888	2	25	theme	solutions	527:535	arg1	electrospinning					498:512	the electrospinning	494:512	the electrospinning of colloidal solutions	494:535	The concentration of the TiO2 NPs in the composite was adjusted to 1.0, 1.5, and 2.0 wt % with respect to polymer concentration used for the electrospinning of colloidal solutions.
31894888	9	26	theme	tissue	1755:1760	arg1	integration					1762:1772	future osseous tissue integration	1740:1772	future osseous tissue integration	1740:1772	Furthermore, the fabricated scaffolds were subjected to analysis in simulated body fluid at 37°C to induce mineralization for future osseous tissue integration.
31894888	5	27	theme	physicochemical	927:941	arg1	properties					943:952	The physicochemical properties	923:952	The physicochemical properties of the nanofibers	923:970	The physicochemical properties of the nanofibers were identified by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) and contact angle meter studies.
31894888	4	28	theme	nanofiber	753:761	arg1	scaffolds					763:771	our nanofiber scaffolds	749:771	our nanofiber scaffolds	749:771	Moreover, to augment our nanofiber scaffolds with antibacterial activity, the in situ deposition approach of using Ag NPs was utilized with varied molar concentrations of 0.14, 0.42, and 0.71 M.
31894888	0	29	theme	future	130:135	arg1	applications					156:167	future tissue engineering applications	130:167	future tissue engineering applications	130:167	Fabrication of multifunctional cellulose/TiO2 /Ag composite nanofibers scaffold with antibacterial and bioactivity properties for future tissue engineering applications.
31894888	2	30	theme	TiO2	382:385	arg1	NPs					387:389	the TiO2 NPs	378:389	the TiO2 NPs	378:389	The concentration of the TiO2 NPs in the composite was adjusted to 1.0, 1.5, and 2.0 wt % with respect to polymer concentration used for the electrospinning of colloidal solutions.
31894888	5	31	theme	meter	1119:1123	arg1	studies					1125:1131	contact angle meter studies	1105:1131	contact angle meter studies	1105:1131	The physicochemical properties of the nanofibers were identified by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) and contact angle meter studies.
31894888	8	32	theme	embryo	1518:1523	arg1	fibroblasts					1525:1535	chicken embryo fibroblasts	1510:1535	chicken embryo fibroblasts	1510:1535	The biocompatibility of the scaffolds was accustomed using chicken embryo fibroblasts, which confirmed their potential role to be used as wound-healing materials.
31894888	0	33	theme	multifunctional	15:29	arg1	composite					50:58	multifunctional cellulose/TiO2 /Ag composite	15:58	multifunctional cellulose/TiO2 /Ag composite	15:58	Fabrication of multifunctional cellulose/TiO2 /Ag composite nanofibers scaffold with antibacterial and bioactivity properties for future tissue engineering applications.
31894888	2	34	theme	wt	442:443	arg1	%					445:445	1.0, 1.5, and 2.0 wt %	424:445	1.0, 1.5, and 2.0 wt %	424:445	The concentration of the TiO2 NPs in the composite was adjusted to 1.0, 1.5, and 2.0 wt % with respect to polymer concentration used for the electrospinning of colloidal solutions.
31894888	0	35	theme	/Ag	46:48	arg1	composite					50:58	multifunctional cellulose/TiO2 /Ag composite	15:58	multifunctional cellulose/TiO2 /Ag composite	15:58	Fabrication of multifunctional cellulose/TiO2 /Ag composite nanofibers scaffold with antibacterial and bioactivity properties for future tissue engineering applications.
31894888	1	36	theme	silver	324:329	arg1	NPs					351:353	NPs	351:353	NPs	351:353	In the present work, a novel strategy was explored to fabricate nanofiber scaffolds consisting of cellulose assimilated with titanium dioxide (TiO2 ) and silver (Ag) nanoparticles (NPs).
31894888	1	36	theme	silver	324:329	arg1	nanoparticles					336:348	silver (Ag) nanoparticles	324:348	silver (Ag) nanoparticles (NPs)	324:354	In the present work, a novel strategy was explored to fabricate nanofiber scaffolds consisting of cellulose assimilated with titanium dioxide (TiO2 ) and silver (Ag) nanoparticles (NPs).
31894888	5	37	theme	scanning	991:998	arg1	SEM					1021:1023	SEM	1021:1023	SEM	1021:1023	The physicochemical properties of the nanofibers were identified by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) and contact angle meter studies.
31894888	5	37	theme	scanning	991:998	arg1	microscopy					1009:1018	scanning electron microscopy	991:1018	scanning electron microscopy (SEM)	991:1024	The physicochemical properties of the nanofibers were identified by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) and contact angle meter studies.
31894888	5	38	dep	Fourier	1067:1073	arg1	transform					1075:1083	transform	1075:1083	transform infrared	1075:1092	The physicochemical properties of the nanofibers were identified by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) and contact angle meter studies.
31894888	10	39	theme	multifunctional	1849:1863	arg1	characteristics					1865:1879	multifunctional characteristics	1849:1879	multifunctional characteristics	1849:1879	These results indicate that fabricated composite nanofiber scaffolds with multifunctional characteristics will have a highest potential as a future candidate for promoting new tissues artificially.
31894888	10	40	theme	new	1947:1949	arg1	tissues					1951:1957	new tissues	1947:1957	new tissues	1947:1957	These results indicate that fabricated composite nanofiber scaffolds with multifunctional characteristics will have a highest potential as a future candidate for promoting new tissues artificially.
31894888	5	41	theme	contact	1105:1111	arg1	studies					1125:1131	contact angle meter studies	1105:1131	contact angle meter studies	1105:1131	The physicochemical properties of the nanofibers were identified by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) and contact angle meter studies.
31894888	8	42	theme	potential	1560:1568	arg1	role					1570:1573	their potential role to be used as wound-healing materials	1554:1611	their potential role to be used as wound-healing materials	1554:1611	The biocompatibility of the scaffolds was accustomed using chicken embryo fibroblasts, which confirmed their potential role to be used as wound-healing materials.
31894888	5	43	theme	nanofibers	961:970	arg1	properties					943:952	The physicochemical properties	923:952	The physicochemical properties of the nanofibers	923:970	The physicochemical properties of the nanofibers were identified by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) and contact angle meter studies.
31894888	3	44	theme	acetyl	646:651	arg1	groups					653:658	the acetyl groups	642:658	the acetyl groups	642:658	The fabricated composite scaffolds were dispensed to alkaline deacetylation using 0.05 M NaOH to remove the acetyl groups in order to generate pure cellulose nanofibers containing TiO2 NPs.
31894888	3	45	theme	pure	681:684	arg1	nanofibers					696:705	pure cellulose nanofibers	681:705	pure cellulose nanofibers containing TiO2 NPs	681:725	The fabricated composite scaffolds were dispensed to alkaline deacetylation using 0.05 M NaOH to remove the acetyl groups in order to generate pure cellulose nanofibers containing TiO2 NPs.
31894888	4	46	theme	Ag	843:844	arg1	NPs					846:848	Ag NPs	843:848	Ag NPs	843:848	Moreover, to augment our nanofiber scaffolds with antibacterial activity, the in situ deposition approach of using Ag NPs was utilized with varied molar concentrations of 0.14, 0.42, and 0.71 M.
31894888	8	47	theme	wound-healing	1589:1601	arg1	materials					1603:1611	wound-healing materials	1589:1611	wound-healing materials	1589:1611	The biocompatibility of the scaffolds was accustomed using chicken embryo fibroblasts, which confirmed their potential role to be used as wound-healing materials.
31894888	6	48	theme	nanofibers	1219:1228	arg1	presence					1156:1163	the presence	1152:1163	the presence of both TiO2 and Ag NPs	1152:1187	This demonstrated the presence of both TiO2 and Ag NPs and complete deacetylation of nanofibers.
31894888	6	48	theme	nanofibers	1219:1228	arg1	deacetylation					1202:1214	complete deacetylation	1193:1214	complete deacetylation of nanofibers	1193:1228	This demonstrated the presence of both TiO2 and Ag NPs and complete deacetylation of nanofibers.
31894888	1	49	theme	titanium	295:302	arg1	TiO2					313:316	TiO2	313:316	TiO2	313:316	In the present work, a novel strategy was explored to fabricate nanofiber scaffolds consisting of cellulose assimilated with titanium dioxide (TiO2 ) and silver (Ag) nanoparticles (NPs).
31894888	1	49	theme	titanium	295:302	arg1	dioxide					304:310	titanium dioxide	295:310	titanium dioxide (TiO2 )	295:318	In the present work, a novel strategy was explored to fabricate nanofiber scaffolds consisting of cellulose assimilated with titanium dioxide (TiO2 ) and silver (Ag) nanoparticles (NPs).
31894888	3	50	theme	TiO2	718:721	arg1	NPs					723:725	TiO2 NPs	718:725	TiO2 NPs	718:725	The fabricated composite scaffolds were dispensed to alkaline deacetylation using 0.05 M NaOH to remove the acetyl groups in order to generate pure cellulose nanofibers containing TiO2 NPs.
31894888	4	51	theme	deposition	814:823	arg1	approach					825:832	the in situ deposition approach	802:832	the in situ deposition approach of using Ag NPs	802:848	Moreover, to augment our nanofiber scaffolds with antibacterial activity, the in situ deposition approach of using Ag NPs was utilized with varied molar concentrations of 0.14, 0.42, and 0.71 M.
31894888	7	52	theme	NPs	1388:1390	arg1	deposition					1371:1380	proper in situ deposition	1356:1380	proper in situ deposition of Ag NPs	1356:1390	The antibacterial efficiency of the nanofibers was scrutinized against Escherichia coli and Staphylococcus aureus, revealing proper in situ deposition of Ag NPs and confirming the nanofibers are antibacterial in nature.
31894888	7	53	from	antibacterial	1426:1438	arg1	nature					1443:1448	nature	1443:1448	nature	1443:1448	The antibacterial efficiency of the nanofibers was scrutinized against Escherichia coli and Staphylococcus aureus, revealing proper in situ deposition of Ag NPs and confirming the nanofibers are antibacterial in nature.
31894888	9	54	theme	simulated	1682:1690	arg1	fluid					1697:1701	simulated body fluid	1682:1701	simulated body fluid at 37°C to induce mineralization for future osseous tissue integration	1682:1772	Furthermore, the fabricated scaffolds were subjected to analysis in simulated body fluid at 37°C to induce mineralization for future osseous tissue integration.
31894888	5	55	theme	electron	1040:1047	arg1	TEM					1061:1063	TEM	1061:1063	TEM	1061:1063	The physicochemical properties of the nanofibers were identified by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) and contact angle meter studies.
31894888	5	55	theme	electron	1040:1047	arg1	microscopy					1049:1058	transmission electron microscopy	1027:1058	transmission electron microscopy (TEM)	1027:1064	The physicochemical properties of the nanofibers were identified by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) and contact angle meter studies.
31894888	10	56	theme	fabricated	1803:1812	arg1	scaffolds					1834:1842	fabricated composite nanofiber scaffolds	1803:1842	fabricated composite nanofiber scaffolds with multifunctional characteristics	1803:1879	These results indicate that fabricated composite nanofiber scaffolds with multifunctional characteristics will have a highest potential as a future candidate for promoting new tissues artificially.
31894888	7	57	from	nature	1443:1448	arg1	antibacterial					1426:1438	antibacterial	1426:1438	antibacterial	1426:1438	The antibacterial efficiency of the nanofibers was scrutinized against Escherichia coli and Staphylococcus aureus, revealing proper in situ deposition of Ag NPs and confirming the nanofibers are antibacterial in nature.
31894888	7	57	from	nature	1443:1448	arg1	nanofibers					1411:1420	the nanofibers	1407:1420	the nanofibers	1407:1420	The antibacterial efficiency of the nanofibers was scrutinized against Escherichia coli and Staphylococcus aureus, revealing proper in situ deposition of Ag NPs and confirming the nanofibers are antibacterial in nature.
31894888	7	58	theme	proper	1356:1361	arg1	deposition					1371:1380	proper in situ deposition	1356:1380	proper in situ deposition of Ag NPs	1356:1390	The antibacterial efficiency of the nanofibers was scrutinized against Escherichia coli and Staphylococcus aureus, revealing proper in situ deposition of Ag NPs and confirming the nanofibers are antibacterial in nature.
31894888	3	59	theme	composite	553:561	arg1	scaffolds					563:571	The fabricated composite scaffolds	538:571	The fabricated composite scaffolds	538:571	The fabricated composite scaffolds were dispensed to alkaline deacetylation using 0.05 M NaOH to remove the acetyl groups in order to generate pure cellulose nanofibers containing TiO2 NPs.
31894888	4	60	theme	0.14	899:902	arg1	concentrations					881:894	varied molar concentrations	868:894	varied molar concentrations of 0.14, 0.42, and 0.71 M	868:920	Moreover, to augment our nanofiber scaffolds with antibacterial activity, the in situ deposition approach of using Ag NPs was utilized with varied molar concentrations of 0.14, 0.42, and 0.71 M.
31894888	4	61	theme	molar	875:879	arg1	concentrations					881:894	varied molar concentrations	868:894	varied molar concentrations of 0.14, 0.42, and 0.71 M	868:920	Moreover, to augment our nanofiber scaffolds with antibacterial activity, the in situ deposition approach of using Ag NPs was utilized with varied molar concentrations of 0.14, 0.42, and 0.71 M.
31894888	3	62	contain	containing	707:716	arg1	nanofibers					696:705	pure cellulose nanofibers	681:705	pure cellulose nanofibers containing TiO2 NPs	681:725	The fabricated composite scaffolds were dispensed to alkaline deacetylation using 0.05 M NaOH to remove the acetyl groups in order to generate pure cellulose nanofibers containing TiO2 NPs.
31894888	3	62	contain	containing	707:716	arg2	NPs					723:725	TiO2 NPs	718:725	TiO2 NPs	718:725	The fabricated composite scaffolds were dispensed to alkaline deacetylation using 0.05 M NaOH to remove the acetyl groups in order to generate pure cellulose nanofibers containing TiO2 NPs.
31894888	0	63	theme	antibacterial	85:97	arg1	properties					115:124	antibacterial and bioactivity properties	85:124	antibacterial and bioactivity properties	85:124	Fabrication of multifunctional cellulose/TiO2 /Ag composite nanofibers scaffold with antibacterial and bioactivity properties for future tissue engineering applications.
31894888	10	64	theme	nanofiber	1824:1832	arg1	scaffolds					1834:1842	fabricated composite nanofiber scaffolds	1803:1842	fabricated composite nanofiber scaffolds with multifunctional characteristics	1803:1879	These results indicate that fabricated composite nanofiber scaffolds with multifunctional characteristics will have a highest potential as a future candidate for promoting new tissues artificially.
31894888	7	65	dep	in	1363:1364	arg1	situ					1366:1369	situ	1366:1369	situ	1366:1369	The antibacterial efficiency of the nanofibers was scrutinized against Escherichia coli and Staphylococcus aureus, revealing proper in situ deposition of Ag NPs and confirming the nanofibers are antibacterial in nature.
31894888	0	66	theme	bioactivity	103:113	arg1	properties					115:124	antibacterial and bioactivity properties	85:124	antibacterial and bioactivity properties	85:124	Fabrication of multifunctional cellulose/TiO2 /Ag composite nanofibers scaffold with antibacterial and bioactivity properties for future tissue engineering applications.
31894888	3	67	theme	alkaline	591:598	arg1	deacetylation					600:612	alkaline deacetylation	591:612	alkaline deacetylation	591:612	The fabricated composite scaffolds were dispensed to alkaline deacetylation using 0.05 M NaOH to remove the acetyl groups in order to generate pure cellulose nanofibers containing TiO2 NPs.
31894888	9	68	theme	osseous	1747:1753	arg1	integration					1762:1772	future osseous tissue integration	1740:1772	future osseous tissue integration	1740:1772	Furthermore, the fabricated scaffolds were subjected to analysis in simulated body fluid at 37°C to induce mineralization for future osseous tissue integration.
31894888	8	69	theme	scaffolds	1479:1487	arg1	biocompatibility					1455:1470	The biocompatibility	1451:1470	The biocompatibility of the scaffolds	1451:1487	The biocompatibility of the scaffolds was accustomed using chicken embryo fibroblasts, which confirmed their potential role to be used as wound-healing materials.
31894888	0	70	theme	tissue	137:142	arg1	applications					156:167	future tissue engineering applications	130:167	future tissue engineering applications	130:167	Fabrication of multifunctional cellulose/TiO2 /Ag composite nanofibers scaffold with antibacterial and bioactivity properties for future tissue engineering applications.
31894888	2	71	theme	polymer	463:469	arg1	concentration					471:483	polymer concentration	463:483	polymer concentration used for the electrospinning of colloidal solutions	463:535	The concentration of the TiO2 NPs in the composite was adjusted to 1.0, 1.5, and 2.0 wt % with respect to polymer concentration used for the electrospinning of colloidal solutions.
31894888	8	72	theme	chicken	1510:1516	arg1	fibroblasts					1525:1535	chicken embryo fibroblasts	1510:1535	chicken embryo fibroblasts	1510:1535	The biocompatibility of the scaffolds was accustomed using chicken embryo fibroblasts, which confirmed their potential role to be used as wound-healing materials.
31894888	2	73	theme	NPs	387:389	arg1	concentration					361:373	The concentration	357:373	The concentration of the TiO2 NPs in the composite	357:406	The concentration of the TiO2 NPs in the composite was adjusted to 1.0, 1.5, and 2.0 wt % with respect to polymer concentration used for the electrospinning of colloidal solutions.
31894888	7	74	theme	nanofibers	1267:1276	arg1	efficiency					1249:1258	The antibacterial efficiency	1231:1258	The antibacterial efficiency of the nanofibers	1231:1276	The antibacterial efficiency of the nanofibers was scrutinized against Escherichia coli and Staphylococcus aureus, revealing proper in situ deposition of Ag NPs and confirming the nanofibers are antibacterial in nature.
31894888	4	75	theme	0.42	905:908	arg1	concentrations					881:894	varied molar concentrations	868:894	varied molar concentrations of 0.14, 0.42, and 0.71 M	868:920	Moreover, to augment our nanofiber scaffolds with antibacterial activity, the in situ deposition approach of using Ag NPs was utilized with varied molar concentrations of 0.14, 0.42, and 0.71 M.
31894888	0	76	theme	cellulose/TiO2	31:44	arg1	composite					50:58	multifunctional cellulose/TiO2 /Ag composite	15:58	multifunctional cellulose/TiO2 /Ag composite	15:58	Fabrication of multifunctional cellulose/TiO2 /Ag composite nanofibers scaffold with antibacterial and bioactivity properties for future tissue engineering applications.
31894888	4	77	used	utilized	854:861	arg2	approach					825:832	the in situ deposition approach	802:832	the in situ deposition approach of using Ag NPs	802:848	Moreover, to augment our nanofiber scaffolds with antibacterial activity, the in situ deposition approach of using Ag NPs was utilized with varied molar concentrations of 0.14, 0.42, and 0.71 M.
31894888	5	78	dep	transform	1075:1083	arg1	infrared					1085:1092	infrared	1085:1092	transform infrared	1075:1092	The physicochemical properties of the nanofibers were identified by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) and contact angle meter studies.
31894888	0	79	theme	composite	50:58	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of multifunctional cellulose/TiO2 /Ag composite	0:58	Fabrication of multifunctional cellulose/TiO2 /Ag composite nanofibers scaffold with antibacterial and bioactivity properties for future tissue engineering applications.
31894888	7	80	theme	antibacterial	1235:1247	arg1	efficiency					1249:1258	The antibacterial efficiency	1231:1258	The antibacterial efficiency of the nanofibers	1231:1276	The antibacterial efficiency of the nanofibers was scrutinized against Escherichia coli and Staphylococcus aureus, revealing proper in situ deposition of Ag NPs and confirming the nanofibers are antibacterial in nature.
31894888	3	81	theme	0.05 M	620:625	arg1	NaOH					627:630	0.05 M NaOH	620:630	0.05 M NaOH	620:630	The fabricated composite scaffolds were dispensed to alkaline deacetylation using 0.05 M NaOH to remove the acetyl groups in order to generate pure cellulose nanofibers containing TiO2 NPs.
31894888	4	82	theme	0.71 M	915:920	arg1	concentrations					881:894	varied molar concentrations	868:894	varied molar concentrations of 0.14, 0.42, and 0.71 M	868:920	Moreover, to augment our nanofiber scaffolds with antibacterial activity, the in situ deposition approach of using Ag NPs was utilized with varied molar concentrations of 0.14, 0.42, and 0.71 M.
31894888	5	83	theme	electron	1000:1007	arg1	SEM					1021:1023	SEM	1021:1023	SEM	1021:1023	The physicochemical properties of the nanofibers were identified by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) and contact angle meter studies.
31894888	5	83	theme	electron	1000:1007	arg1	microscopy					1009:1018	scanning electron microscopy	991:1018	scanning electron microscopy (SEM)	991:1024	The physicochemical properties of the nanofibers were identified by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) and contact angle meter studies.
31894888	4	84	theme	in	806:807	arg1	approach					825:832	the in situ deposition approach	802:832	the in situ deposition approach of using Ag NPs	802:848	Moreover, to augment our nanofiber scaffolds with antibacterial activity, the in situ deposition approach of using Ag NPs was utilized with varied molar concentrations of 0.14, 0.42, and 0.71 M.
31894888	5	85	theme	angle	1113:1117	arg1	studies					1125:1131	contact angle meter studies	1105:1131	contact angle meter studies	1105:1131	The physicochemical properties of the nanofibers were identified by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR) and contact angle meter studies.
31894888	10	86	theme	highest	1893:1899	arg1	candidate					1923:1931	a future candidate	1914:1931	a future candidate for promoting new tissues artificially	1914:1970	These results indicate that fabricated composite nanofiber scaffolds with multifunctional characteristics will have a highest potential as a future candidate for promoting new tissues artificially.
31894888	10	86	theme	highest	1893:1899	arg1	potential					1901:1909	a highest potential	1891:1909	a highest potential	1891:1909	These results indicate that fabricated composite nanofiber scaffolds with multifunctional characteristics will have a highest potential as a future candidate for promoting new tissues artificially.
31894888	0	87	with	scaffold	71:78	arg1	properties					115:124	antibacterial and bioactivity properties	85:124	antibacterial and bioactivity properties	85:124	Fabrication of multifunctional cellulose/TiO2 /Ag composite nanofibers scaffold with antibacterial and bioactivity properties for future tissue engineering applications.
31894888	10	88	contain	have	1886:1889	arg2	potential					1901:1909	a highest potential	1891:1909	a highest potential	1891:1909	These results indicate that fabricated composite nanofiber scaffolds with multifunctional characteristics will have a highest potential as a future candidate for promoting new tissues artificially.
31894888	10	88	contain	have	1886:1889	arg1	scaffolds					1834:1842	fabricated composite nanofiber scaffolds	1803:1842	fabricated composite nanofiber scaffolds with multifunctional characteristics	1803:1879	These results indicate that fabricated composite nanofiber scaffolds with multifunctional characteristics will have a highest potential as a future candidate for promoting new tissues artificially.
31894888	10	88	contain	have	1886:1889	arg2	candidate					1923:1931	a future candidate	1914:1931	a future candidate for promoting new tissues artificially	1914:1970	These results indicate that fabricated composite nanofiber scaffolds with multifunctional characteristics will have a highest potential as a future candidate for promoting new tissues artificially.
31894888	4	89	theme	antibacterial	778:790	arg1	activity					792:799	antibacterial activity	778:799	antibacterial activity	778:799	Moreover, to augment our nanofiber scaffolds with antibacterial activity, the in situ deposition approach of using Ag NPs was utilized with varied molar concentrations of 0.14, 0.42, and 0.71 M.
32276092	1	0	dep	%	263:263	arg1	6					262:262	6	262:262	6	262:262	In this paper, we report the development and viscoelastic properties of hyaluronic acid formulations (HA5, HA30, and HA60, containing 0.5, 3, and 6% HA, respectively) loaded with carvacrol prodrugs (WSCPS) with antibacterial properties.
32276092	1	0	dep	%	263:263	arg1	3					255:255	3	255:255	3	255:255	In this paper, we report the development and viscoelastic properties of hyaluronic acid formulations (HA5, HA30, and HA60, containing 0.5, 3, and 6% HA, respectively) loaded with carvacrol prodrugs (WSCPS) with antibacterial properties.
32276092	1	1	theme	viscoelastic	161:172	arg1	properties					174:183	viscoelastic properties	161:183	viscoelastic properties	161:183	In this paper, we report the development and viscoelastic properties of hyaluronic acid formulations (HA5, HA30, and HA60, containing 0.5, 3, and 6% HA, respectively) loaded with carvacrol prodrugs (WSCPS) with antibacterial properties.
32276092	0	2	theme	antibacterial	90:102	arg1	properties					104:113	antibacterial properties	90:113	antibacterial properties	90:113	Viscoelastic behaviour of hyaluronic acid formulations containing carvacrol prodrugs with antibacterial properties.
32276092	1	3	theme	carvacrol	295:303	arg1	WSCPS					315:319	WSCPS	315:319	WSCPS	315:319	In this paper, we report the development and viscoelastic properties of hyaluronic acid formulations (HA5, HA30, and HA60, containing 0.5, 3, and 6% HA, respectively) loaded with carvacrol prodrugs (WSCPS) with antibacterial properties.
32276092	1	3	theme	carvacrol	295:303	arg1	prodrugs					305:312	carvacrol prodrugs	295:312	carvacrol prodrugs (WSCPS)	295:320	In this paper, we report the development and viscoelastic properties of hyaluronic acid formulations (HA5, HA30, and HA60, containing 0.5, 3, and 6% HA, respectively) loaded with carvacrol prodrugs (WSCPS) with antibacterial properties.
32276092	4	4	theme	suitable	970:977	arg1	texture					962:968	a texture	960:968	a good percentage of prodrug release (e.g., 30 and 60% for WSCP1 and 2, respectively) as well as a texture suitable for topical administration to treat wounds and/or skin infections	863:1043	Considering these data, HA30 is a useful formulation which guarantees a good percentage of prodrug release (e.g., 30 and 60% for WSCP1 and 2, respectively) as well as a texture suitable for topical administration to treat wounds and/or skin infections.
32276092	3	5	theme	fluid	774:778	arg1	consistency					780:790	a fluid consistency	772:790	a fluid consistency	772:790	Moreover, rheological analyses showed that HA30 composites exhibited a semi-solid consistency, while HA5 formulations possessed a fluid consistency.
32276092	4	6	theme	useful	827:832	arg1	HA30					817:820	HA30	817:820	HA30	817:820	Considering these data, HA30 is a useful formulation which guarantees a good percentage of prodrug release (e.g., 30 and 60% for WSCP1 and 2, respectively) as well as a texture suitable for topical administration to treat wounds and/or skin infections.
32276092	4	6	theme	useful	827:832	arg1	formulation					834:844	a useful formulation	825:844	a useful formulation which guarantees a good percentage of prodrug release (e.g., 30 and 60% for WSCP1 and 2, respectively) as well as a texture suitable for topical administration to treat wounds and/or skin infections	825:1043	Considering these data, HA30 is a useful formulation which guarantees a good percentage of prodrug release (e.g., 30 and 60% for WSCP1 and 2, respectively) as well as a texture suitable for topical administration to treat wounds and/or skin infections.
32276092	2	7	theme	concentration	475:487	arg1	values					495:500	the best minimum inhibitory concentration (MIC) values	447:500	the best minimum inhibitory concentration (MIC) values against Enterococcus faecium (128 mg/L) and Enterococcus faecalis (256 mg/L)	447:577	Notably, antimicrobial studies revealed that WSCP1-2 in both HA5 and HA30 formulations showed the best minimum inhibitory concentration (MIC) values against Enterococcus faecium (128 mg/L) and Enterococcus faecalis (256 mg/L) compared to those of carvacrol alone or in formulations with HA.
32276092	2	8	theme	inhibitory	464:473	arg1	concentration					475:487	the best minimum inhibitory concentration	447:487	the best minimum inhibitory concentration (MIC) values against Enterococcus faecium (128 mg/L) and Enterococcus faecalis (256 mg/L)	447:577	Notably, antimicrobial studies revealed that WSCP1-2 in both HA5 and HA30 formulations showed the best minimum inhibitory concentration (MIC) values against Enterococcus faecium (128 mg/L) and Enterococcus faecalis (256 mg/L) compared to those of carvacrol alone or in formulations with HA.
32276092	2	8	theme	inhibitory	464:473	arg1	MIC					490:492	MIC	490:492	MIC	490:492	Notably, antimicrobial studies revealed that WSCP1-2 in both HA5 and HA30 formulations showed the best minimum inhibitory concentration (MIC) values against Enterococcus faecium (128 mg/L) and Enterococcus faecalis (256 mg/L) compared to those of carvacrol alone or in formulations with HA.
32276092	1	9	dep	HA	265:266	arg1	%					263:263	%	263:263	%	263:263	In this paper, we report the development and viscoelastic properties of hyaluronic acid formulations (HA5, HA30, and HA60, containing 0.5, 3, and 6% HA, respectively) loaded with carvacrol prodrugs (WSCPS) with antibacterial properties.
32276092	1	10	theme	hyaluronic	188:197	arg1	HA60					233:236	HA60	233:236	HA60	233:236	In this paper, we report the development and viscoelastic properties of hyaluronic acid formulations (HA5, HA30, and HA60, containing 0.5, 3, and 6% HA, respectively) loaded with carvacrol prodrugs (WSCPS) with antibacterial properties.
32276092	1	10	theme	hyaluronic	188:197	arg1	HA5					218:220	HA5	218:220	HA5	218:220	In this paper, we report the development and viscoelastic properties of hyaluronic acid formulations (HA5, HA30, and HA60, containing 0.5, 3, and 6% HA, respectively) loaded with carvacrol prodrugs (WSCPS) with antibacterial properties.
32276092	1	10	theme	hyaluronic	188:197	arg1	formulations					204:215	hyaluronic acid formulations	188:215	hyaluronic acid formulations (HA5, HA30, and HA60, containing 0.5, 3, and 6% HA, respectively) loaded with carvacrol prodrugs (WSCPS) with antibacterial properties	188:350	In this paper, we report the development and viscoelastic properties of hyaluronic acid formulations (HA5, HA30, and HA60, containing 0.5, 3, and 6% HA, respectively) loaded with carvacrol prodrugs (WSCPS) with antibacterial properties.
32276092	1	10	theme	hyaluronic	188:197	arg1	HA30					223:226	HA30	223:226	HA30	223:226	In this paper, we report the development and viscoelastic properties of hyaluronic acid formulations (HA5, HA30, and HA60, containing 0.5, 3, and 6% HA, respectively) loaded with carvacrol prodrugs (WSCPS) with antibacterial properties.
32276092	4	11	dep	%	916:916	arg1	e.g.					901:904	e.g.	901:904	e.g.	901:904	Considering these data, HA30 is a useful formulation which guarantees a good percentage of prodrug release (e.g., 30 and 60% for WSCP1 and 2, respectively) as well as a texture suitable for topical administration to treat wounds and/or skin infections.
32276092	1	12	theme	acid	199:202	arg1	HA60					233:236	HA60	233:236	HA60	233:236	In this paper, we report the development and viscoelastic properties of hyaluronic acid formulations (HA5, HA30, and HA60, containing 0.5, 3, and 6% HA, respectively) loaded with carvacrol prodrugs (WSCPS) with antibacterial properties.
32276092	1	12	theme	acid	199:202	arg1	HA5					218:220	HA5	218:220	HA5	218:220	In this paper, we report the development and viscoelastic properties of hyaluronic acid formulations (HA5, HA30, and HA60, containing 0.5, 3, and 6% HA, respectively) loaded with carvacrol prodrugs (WSCPS) with antibacterial properties.
32276092	1	12	theme	acid	199:202	arg1	formulations					204:215	hyaluronic acid formulations	188:215	hyaluronic acid formulations (HA5, HA30, and HA60, containing 0.5, 3, and 6% HA, respectively) loaded with carvacrol prodrugs (WSCPS) with antibacterial properties	188:350	In this paper, we report the development and viscoelastic properties of hyaluronic acid formulations (HA5, HA30, and HA60, containing 0.5, 3, and 6% HA, respectively) loaded with carvacrol prodrugs (WSCPS) with antibacterial properties.
32276092	1	12	theme	acid	199:202	arg1	HA30					223:226	HA30	223:226	HA30	223:226	In this paper, we report the development and viscoelastic properties of hyaluronic acid formulations (HA5, HA30, and HA60, containing 0.5, 3, and 6% HA, respectively) loaded with carvacrol prodrugs (WSCPS) with antibacterial properties.
32276092	0	13	theme	Viscoelastic	0:11	arg1	behaviour					13:21	Viscoelastic behaviour	0:21	Viscoelastic behaviour of hyaluronic acid formulations	0:53	Viscoelastic behaviour of hyaluronic acid formulations containing carvacrol prodrugs with antibacterial properties.
32276092	1	14	theme	formulations	204:215	arg1	development					145:155	the development	141:155	the development	141:155	In this paper, we report the development and viscoelastic properties of hyaluronic acid formulations (HA5, HA30, and HA60, containing 0.5, 3, and 6% HA, respectively) loaded with carvacrol prodrugs (WSCPS) with antibacterial properties.
32276092	1	14	theme	formulations	204:215	arg1	properties					174:183	viscoelastic properties	161:183	viscoelastic properties	161:183	In this paper, we report the development and viscoelastic properties of hyaluronic acid formulations (HA5, HA30, and HA60, containing 0.5, 3, and 6% HA, respectively) loaded with carvacrol prodrugs (WSCPS) with antibacterial properties.
32276092	0	15	theme	hyaluronic	26:35	arg1	formulations					42:53	hyaluronic acid formulations	26:53	hyaluronic acid formulations	26:53	Viscoelastic behaviour of hyaluronic acid formulations containing carvacrol prodrugs with antibacterial properties.
32276092	3	16	theme	HA30	687:690	arg1	composites					692:701	HA30 composites	687:701	HA30 composites	687:701	Moreover, rheological analyses showed that HA30 composites exhibited a semi-solid consistency, while HA5 formulations possessed a fluid consistency.
32276092	1	17	theme	antibacterial	327:339	arg1	properties					341:350	antibacterial properties	327:350	antibacterial properties	327:350	In this paper, we report the development and viscoelastic properties of hyaluronic acid formulations (HA5, HA30, and HA60, containing 0.5, 3, and 6% HA, respectively) loaded with carvacrol prodrugs (WSCPS) with antibacterial properties.
32276092	4	18	theme	skin	1029:1032	arg1	infections					1034:1043	skin infections	1029:1043	skin infections	1029:1043	Considering these data, HA30 is a useful formulation which guarantees a good percentage of prodrug release (e.g., 30 and 60% for WSCP1 and 2, respectively) as well as a texture suitable for topical administration to treat wounds and/or skin infections.
32276092	0	19	theme	formulations	42:53	arg1	behaviour					13:21	Viscoelastic behaviour	0:21	Viscoelastic behaviour of hyaluronic acid formulations	0:53	Viscoelastic behaviour of hyaluronic acid formulations containing carvacrol prodrugs with antibacterial properties.
32276092	4	20	theme	release	892:898	arg1	texture					962:968	a texture	960:968	a good percentage of prodrug release (e.g., 30 and 60% for WSCP1 and 2, respectively) as well as a texture suitable for topical administration to treat wounds and/or skin infections	863:1043	Considering these data, HA30 is a useful formulation which guarantees a good percentage of prodrug release (e.g., 30 and 60% for WSCP1 and 2, respectively) as well as a texture suitable for topical administration to treat wounds and/or skin infections.
32276092	4	20	theme	release	892:898	arg1	percentage					870:879	a good percentage	863:879	a good percentage of prodrug release (e.g., 30 and 60% for WSCP1 and 2, respectively) as well as a texture suitable for topical administration to treat wounds and/or skin infections	863:1043	Considering these data, HA30 is a useful formulation which guarantees a good percentage of prodrug release (e.g., 30 and 60% for WSCP1 and 2, respectively) as well as a texture suitable for topical administration to treat wounds and/or skin infections.
32276092	4	21	theme	prodrug	884:890	arg1	release					892:898	prodrug release	884:898	prodrug release	884:898	Considering these data, HA30 is a useful formulation which guarantees a good percentage of prodrug release (e.g., 30 and 60% for WSCP1 and 2, respectively) as well as a texture suitable for topical administration to treat wounds and/or skin infections.
32276092	2	22	theme	antimicrobial	362:374	arg1	studies					376:382	antimicrobial studies	362:382	antimicrobial studies	362:382	Notably, antimicrobial studies revealed that WSCP1-2 in both HA5 and HA30 formulations showed the best minimum inhibitory concentration (MIC) values against Enterococcus faecium (128 mg/L) and Enterococcus faecalis (256 mg/L) compared to those of carvacrol alone or in formulations with HA.
32276092	0	23	theme	acid	37:40	arg1	formulations					42:53	hyaluronic acid formulations	26:53	hyaluronic acid formulations	26:53	Viscoelastic behaviour of hyaluronic acid formulations containing carvacrol prodrugs with antibacterial properties.
32276092	2	24	theme	HA30	422:425	arg1	formulations					427:438	both HA5 and HA30 formulations	409:438	formulations	427:438	Notably, antimicrobial studies revealed that WSCP1-2 in both HA5 and HA30 formulations showed the best minimum inhibitory concentration (MIC) values against Enterococcus faecium (128 mg/L) and Enterococcus faecalis (256 mg/L) compared to those of carvacrol alone or in formulations with HA.
32276092	1	25	dep	formulations	204:215	arg1	HA60					233:236	HA60	233:236	HA60	233:236	In this paper, we report the development and viscoelastic properties of hyaluronic acid formulations (HA5, HA30, and HA60, containing 0.5, 3, and 6% HA, respectively) loaded with carvacrol prodrugs (WSCPS) with antibacterial properties.
32276092	1	25	dep	formulations	204:215	arg1	HA5					218:220	HA5	218:220	HA5	218:220	In this paper, we report the development and viscoelastic properties of hyaluronic acid formulations (HA5, HA30, and HA60, containing 0.5, 3, and 6% HA, respectively) loaded with carvacrol prodrugs (WSCPS) with antibacterial properties.
32276092	1	25	dep	formulations	204:215	arg1	formulations					204:215	hyaluronic acid formulations	188:215	hyaluronic acid formulations (HA5, HA30, and HA60, containing 0.5, 3, and 6% HA, respectively) loaded with carvacrol prodrugs (WSCPS) with antibacterial properties	188:350	In this paper, we report the development and viscoelastic properties of hyaluronic acid formulations (HA5, HA30, and HA60, containing 0.5, 3, and 6% HA, respectively) loaded with carvacrol prodrugs (WSCPS) with antibacterial properties.
32276092	1	25	dep	formulations	204:215	arg1	HA30					223:226	HA30	223:226	HA30	223:226	In this paper, we report the development and viscoelastic properties of hyaluronic acid formulations (HA5, HA30, and HA60, containing 0.5, 3, and 6% HA, respectively) loaded with carvacrol prodrugs (WSCPS) with antibacterial properties.
32276092	2	26	with	formulations	622:633	arg1	HA					640:641	HA	640:641	HA	640:641	Notably, antimicrobial studies revealed that WSCP1-2 in both HA5 and HA30 formulations showed the best minimum inhibitory concentration (MIC) values against Enterococcus faecium (128 mg/L) and Enterococcus faecalis (256 mg/L) compared to those of carvacrol alone or in formulations with HA.
32276092	0	27	theme	carvacrol	66:74	arg1	prodrugs					76:83	carvacrol prodrugs	66:83	carvacrol prodrugs	66:83	Viscoelastic behaviour of hyaluronic acid formulations containing carvacrol prodrugs with antibacterial properties.
32276092	3	28	theme	rheological	654:664	arg1	analyses					666:673	rheological analyses	654:673	rheological analyses	654:673	Moreover, rheological analyses showed that HA30 composites exhibited a semi-solid consistency, while HA5 formulations possessed a fluid consistency.
32276092	2	29	theme	HA5	414:416	arg1	formulations					427:438	both HA5 and HA30 formulations	409:438	formulations	427:438	Notably, antimicrobial studies revealed that WSCP1-2 in both HA5 and HA30 formulations showed the best minimum inhibitory concentration (MIC) values against Enterococcus faecium (128 mg/L) and Enterococcus faecalis (256 mg/L) compared to those of carvacrol alone or in formulations with HA.
32276092	3	30	theme	semi-solid	715:724	arg1	consistency					726:736	a semi-solid consistency	713:736	a semi-solid consistency	713:736	Moreover, rheological analyses showed that HA30 composites exhibited a semi-solid consistency, while HA5 formulations possessed a fluid consistency.
32276092	2	31	dep	showed	440:445	arg1	compared					579:586	compared	579:586	showed the best minimum inhibitory concentration (MIC) values against Enterococcus faecium (128 mg/L) and Enterococcus faecalis (256 mg/L) compared to those of carvacrol alone or in formulations with HA	440:641	Notably, antimicrobial studies revealed that WSCP1-2 in both HA5 and HA30 formulations showed the best minimum inhibitory concentration (MIC) values against Enterococcus faecium (128 mg/L) and Enterococcus faecalis (256 mg/L) compared to those of carvacrol alone or in formulations with HA.
32276092	4	32	theme	topical	983:989	arg1	administration					991:1004	topical administration	983:1004	topical administration to treat wounds and/or skin infections	983:1043	Considering these data, HA30 is a useful formulation which guarantees a good percentage of prodrug release (e.g., 30 and 60% for WSCP1 and 2, respectively) as well as a texture suitable for topical administration to treat wounds and/or skin infections.
32276092	3	33	contain	possessed	762:770	arg2	consistency					780:790	a fluid consistency	772:790	a fluid consistency	772:790	Moreover, rheological analyses showed that HA30 composites exhibited a semi-solid consistency, while HA5 formulations possessed a fluid consistency.
32276092	3	33	contain	possessed	762:770	arg1	formulations					749:760	HA5 formulations	745:760	HA5 formulations	745:760	Moreover, rheological analyses showed that HA30 composites exhibited a semi-solid consistency, while HA5 formulations possessed a fluid consistency.
32276092	4	34	theme	good	865:868	arg1	percentage					870:879	a good percentage	863:879	a good percentage of prodrug release (e.g., 30 and 60% for WSCP1 and 2, respectively) as well as a texture suitable for topical administration to treat wounds and/or skin infections	863:1043	Considering these data, HA30 is a useful formulation which guarantees a good percentage of prodrug release (e.g., 30 and 60% for WSCP1 and 2, respectively) as well as a texture suitable for topical administration to treat wounds and/or skin infections.
32276092	2	35	theme	minimum	456:462	arg1	concentration					475:487	the best minimum inhibitory concentration	447:487	the best minimum inhibitory concentration (MIC) values against Enterococcus faecium (128 mg/L) and Enterococcus faecalis (256 mg/L)	447:577	Notably, antimicrobial studies revealed that WSCP1-2 in both HA5 and HA30 formulations showed the best minimum inhibitory concentration (MIC) values against Enterococcus faecium (128 mg/L) and Enterococcus faecalis (256 mg/L) compared to those of carvacrol alone or in formulations with HA.
32276092	2	35	theme	minimum	456:462	arg1	MIC					490:492	MIC	490:492	MIC	490:492	Notably, antimicrobial studies revealed that WSCP1-2 in both HA5 and HA30 formulations showed the best minimum inhibitory concentration (MIC) values against Enterococcus faecium (128 mg/L) and Enterococcus faecalis (256 mg/L) compared to those of carvacrol alone or in formulations with HA.
32276092	3	36	theme	HA5	745:747	arg1	formulations					749:760	HA5 formulations	745:760	HA5 formulations	745:760	Moreover, rheological analyses showed that HA30 composites exhibited a semi-solid consistency, while HA5 formulations possessed a fluid consistency.
32276092	2	37	theme	best	451:454	arg1	concentration					475:487	the best minimum inhibitory concentration	447:487	the best minimum inhibitory concentration (MIC) values against Enterococcus faecium (128 mg/L) and Enterococcus faecalis (256 mg/L)	447:577	Notably, antimicrobial studies revealed that WSCP1-2 in both HA5 and HA30 formulations showed the best minimum inhibitory concentration (MIC) values against Enterococcus faecium (128 mg/L) and Enterococcus faecalis (256 mg/L) compared to those of carvacrol alone or in formulations with HA.
32276092	2	37	theme	best	451:454	arg1	MIC					490:492	MIC	490:492	MIC	490:492	Notably, antimicrobial studies revealed that WSCP1-2 in both HA5 and HA30 formulations showed the best minimum inhibitory concentration (MIC) values against Enterococcus faecium (128 mg/L) and Enterococcus faecalis (256 mg/L) compared to those of carvacrol alone or in formulations with HA.
32276092	2	38	from	WSCP1-2	398:404	arg1	formulations					427:438	both HA5 and HA30 formulations	409:438	formulations	427:438	Notably, antimicrobial studies revealed that WSCP1-2 in both HA5 and HA30 formulations showed the best minimum inhibitory concentration (MIC) values against Enterococcus faecium (128 mg/L) and Enterococcus faecalis (256 mg/L) compared to those of carvacrol alone or in formulations with HA.
32276092	1	39	contain	containing	239:248	arg1	HA60					233:236	HA60	233:236	HA60	233:236	In this paper, we report the development and viscoelastic properties of hyaluronic acid formulations (HA5, HA30, and HA60, containing 0.5, 3, and 6% HA, respectively) loaded with carvacrol prodrugs (WSCPS) with antibacterial properties.
32276092	1	39	contain	containing	239:248	arg1	formulations					204:215	hyaluronic acid formulations	188:215	hyaluronic acid formulations (HA5, HA30, and HA60, containing 0.5, 3, and 6% HA, respectively) loaded with carvacrol prodrugs (WSCPS) with antibacterial properties	188:350	In this paper, we report the development and viscoelastic properties of hyaluronic acid formulations (HA5, HA30, and HA60, containing 0.5, 3, and 6% HA, respectively) loaded with carvacrol prodrugs (WSCPS) with antibacterial properties.
32276092	1	39	contain	containing	239:248	arg2	HA					265:266	0.5, 3, and 6% HA	250:266	0.5, 3, and 6% HA	250:266	In this paper, we report the development and viscoelastic properties of hyaluronic acid formulations (HA5, HA30, and HA60, containing 0.5, 3, and 6% HA, respectively) loaded with carvacrol prodrugs (WSCPS) with antibacterial properties.
34237373	2	0	with	extraction	656:665	arg1	cellulase					672:680	cellulase	672:680	cellulase (~4%)	672:686	Except for pectin from raspberry, conventional citric acid extraction led to the highest extraction yield (~8%) and, for the same berries, the lowest pectin recovery was found for the extraction with cellulase (~4%).
34237373	2	0	with	extraction	656:665	arg1	%					685:685	~4%	683:685	~4%	683:685	Except for pectin from raspberry, conventional citric acid extraction led to the highest extraction yield (~8%) and, for the same berries, the lowest pectin recovery was found for the extraction with cellulase (~4%).
34237373	6	1	located	found	1396:1400	arg2	differences					1384:1394	the differences	1380:1394	the differences found in their physicochemical and techno-functional characteristics	1380:1463	This has been evidenced by the differences found in their physicochemical and techno-functional characteristics.
34237373	6	1	located	found	1396:1400	arg1	characteristics					1449:1463	their physicochemical and techno-functional characteristics	1405:1463	their physicochemical and techno-functional characteristics	1405:1463	This has been evidenced by the differences found in their physicochemical and techno-functional characteristics.
34237373	1	2	from	redcurrant	355:364	arg1	pectin					306:311	pectin	306:311	pectin from raspberry, blueberry, strawberry and redcurrant	306:364	Three non-conventional extraction techniques (enzyme-assisted with cellulase, citric acid ultrasound-assisted and enzyme-ultrasound-assisted treatment) and conventional citric acid extraction were applied to obtain pectin from raspberry, blueberry, strawberry and redcurrant, and were compared in terms of extraction yields and physicochemical properties of the extracted pectins.
34237373	3	3	theme	structural	703:712	arg1	characteristics					714:728	the structural characteristics	699:728	the structural characteristics of pectins	699:739	Regarding the structural characteristics of pectins, enzymatically extracted pectins from redcurrant and strawberry exhibited the highest levels of galacturonic acid (≥73%) whereas, in general, this monosaccharide was found from 51 to 69% in the rest of samples.
34237373	1	4	theme	citric	169:174	arg1	acid					176:179	citric acid ultrasound-assisted	169:199	citric acid ultrasound-assisted	169:199	Three non-conventional extraction techniques (enzyme-assisted with cellulase, citric acid ultrasound-assisted and enzyme-ultrasound-assisted treatment) and conventional citric acid extraction were applied to obtain pectin from raspberry, blueberry, strawberry and redcurrant, and were compared in terms of extraction yields and physicochemical properties of the extracted pectins.
34237373	5	5	theme	pectins	1208:1214	arg1	range					1166:1170	the wide range	1157:1170	the wide range of molecular weight	1157:1190	The different monomeric composition and the wide range of molecular weight of the obtained pectins pointed out their usefulness in different potential food applications (e.g., thickening, gelling ingredients) and biological activities.
34237373	5	5	theme	pectins	1208:1214	arg1	composition					1141:1151	different monomeric composition	1121:1151	different monomeric composition	1121:1151	The different monomeric composition and the wide range of molecular weight of the obtained pectins pointed out their usefulness in different potential food applications (e.g., thickening, gelling ingredients) and biological activities.
34237373	3	6	theme	galacturonic	837:848	arg1	%					859:859	≥73%	856:859	≥73%	856:859	Regarding the structural characteristics of pectins, enzymatically extracted pectins from redcurrant and strawberry exhibited the highest levels of galacturonic acid (≥73%) whereas, in general, this monosaccharide was found from 51 to 69% in the rest of samples.
34237373	3	6	theme	galacturonic	837:848	arg1	acid					850:853	galacturonic acid	837:853	galacturonic acid (≥73%)	837:860	Regarding the structural characteristics of pectins, enzymatically extracted pectins from redcurrant and strawberry exhibited the highest levels of galacturonic acid (≥73%) whereas, in general, this monosaccharide was found from 51 to 69% in the rest of samples.
34237373	0	7	theme	extraction	68:77	arg1	techniques					79:88	Conventional and non-conventional extraction techniques	34:88	Berry fruits as source of pectin: Conventional and non-conventional extraction techniques.	0:89	Berry fruits as source of pectin: Conventional and non-conventional extraction techniques.
34237373	2	8	theme	pectin	622:627	arg1	recovery					629:636	the lowest pectin recovery	611:636	the lowest pectin recovery	611:636	Except for pectin from raspberry, conventional citric acid extraction led to the highest extraction yield (~8%) and, for the same berries, the lowest pectin recovery was found for the extraction with cellulase (~4%).
34237373	4	9	theme	pectin	1109:1114	arg1	obtainment					1089:1098	the obtainment	1085:1098	the obtainment of purer pectin	1085:1114	Although, ultrasound-assisted extraction did not improve pectin yield, it minimized the levels of "non-pectic" components leading to the obtainment of purer pectin.
34237373	6	10	theme	physicochemical	1411:1425	arg1	characteristics					1449:1463	their physicochemical and techno-functional characteristics	1405:1463	their physicochemical and techno-functional characteristics	1405:1463	This has been evidenced by the differences found in their physicochemical and techno-functional characteristics.
34237373	3	11	theme	acid	850:853	arg1	levels					827:832	the highest levels	815:832	the highest levels of galacturonic acid (≥73%)	815:860	Regarding the structural characteristics of pectins, enzymatically extracted pectins from redcurrant and strawberry exhibited the highest levels of galacturonic acid (≥73%) whereas, in general, this monosaccharide was found from 51 to 69% in the rest of samples.
34237373	1	12	from	strawberry	340:349	arg1	pectin					306:311	pectin	306:311	pectin from raspberry, blueberry, strawberry and redcurrant	306:364	Three non-conventional extraction techniques (enzyme-assisted with cellulase, citric acid ultrasound-assisted and enzyme-ultrasound-assisted treatment) and conventional citric acid extraction were applied to obtain pectin from raspberry, blueberry, strawberry and redcurrant, and were compared in terms of extraction yields and physicochemical properties of the extracted pectins.
34237373	5	13	from	usefulness	1234:1243	arg1	applications					1273:1284	different potential food applications	1248:1284	different potential food applications (e.g., thickening, gelling ingredients)	1248:1324	The different monomeric composition and the wide range of molecular weight of the obtained pectins pointed out their usefulness in different potential food applications (e.g., thickening, gelling ingredients) and biological activities.
34237373	5	13	from	usefulness	1234:1243	arg1	activities					1341:1350	biological activities	1330:1350	biological activities	1330:1350	The different monomeric composition and the wide range of molecular weight of the obtained pectins pointed out their usefulness in different potential food applications (e.g., thickening, gelling ingredients) and biological activities.
34237373	5	13	from	usefulness	1234:1243	arg1	ingredients					1313:1323	gelling ingredients	1305:1323	gelling ingredients	1305:1323	The different monomeric composition and the wide range of molecular weight of the obtained pectins pointed out their usefulness in different potential food applications (e.g., thickening, gelling ingredients) and biological activities.
34237373	2	14	theme	extraction	561:570	arg1	%					581:581	~8%	579:581	~8%	579:581	Except for pectin from raspberry, conventional citric acid extraction led to the highest extraction yield (~8%) and, for the same berries, the lowest pectin recovery was found for the extraction with cellulase (~4%).
34237373	2	14	theme	extraction	561:570	arg1	yield					572:576	the highest extraction yield	549:576	the highest extraction yield (~8%)	549:582	Except for pectin from raspberry, conventional citric acid extraction led to the highest extraction yield (~8%) and, for the same berries, the lowest pectin recovery was found for the extraction with cellulase (~4%).
34237373	2	15	theme	same	597:600	arg1	berries					602:608	the same berries	593:608	the same berries	593:608	Except for pectin from raspberry, conventional citric acid extraction led to the highest extraction yield (~8%) and, for the same berries, the lowest pectin recovery was found for the extraction with cellulase (~4%).
34237373	5	16	theme	gelling	1305:1311	arg1	applications					1273:1284	different potential food applications	1248:1284	different potential food applications (e.g., thickening, gelling ingredients)	1248:1324	The different monomeric composition and the wide range of molecular weight of the obtained pectins pointed out their usefulness in different potential food applications (e.g., thickening, gelling ingredients) and biological activities.
34237373	5	16	theme	gelling	1305:1311	arg1	ingredients					1313:1323	gelling ingredients	1305:1323	gelling ingredients	1305:1323	The different monomeric composition and the wide range of molecular weight of the obtained pectins pointed out their usefulness in different potential food applications (e.g., thickening, gelling ingredients) and biological activities.
34237373	3	17	dep	69	924:925	arg1	to					921:922	to	921:922	to	921:922	Regarding the structural characteristics of pectins, enzymatically extracted pectins from redcurrant and strawberry exhibited the highest levels of galacturonic acid (≥73%) whereas, in general, this monosaccharide was found from 51 to 69% in the rest of samples.
34237373	2	18	theme	highest	553:559	arg1	%					581:581	~8%	579:581	~8%	579:581	Except for pectin from raspberry, conventional citric acid extraction led to the highest extraction yield (~8%) and, for the same berries, the lowest pectin recovery was found for the extraction with cellulase (~4%).
34237373	2	18	theme	highest	553:559	arg1	yield					572:576	the highest extraction yield	549:576	the highest extraction yield (~8%)	549:582	Except for pectin from raspberry, conventional citric acid extraction led to the highest extraction yield (~8%) and, for the same berries, the lowest pectin recovery was found for the extraction with cellulase (~4%).
34237373	2	19	from	raspberry	495:503	arg1	pectin					483:488	pectin	483:488	pectin from raspberry	483:503	Except for pectin from raspberry, conventional citric acid extraction led to the highest extraction yield (~8%) and, for the same berries, the lowest pectin recovery was found for the extraction with cellulase (~4%).
34237373	1	20	from	blueberry	329:337	arg1	pectin					306:311	pectin	306:311	pectin from raspberry, blueberry, strawberry and redcurrant	306:364	Three non-conventional extraction techniques (enzyme-assisted with cellulase, citric acid ultrasound-assisted and enzyme-ultrasound-assisted treatment) and conventional citric acid extraction were applied to obtain pectin from raspberry, blueberry, strawberry and redcurrant, and were compared in terms of extraction yields and physicochemical properties of the extracted pectins.
34237373	1	21	dep	techniques	125:134	arg1	enzyme-assisted					137:151	enzyme-assisted	137:151	enzyme-assisted	137:151	Three non-conventional extraction techniques (enzyme-assisted with cellulase, citric acid ultrasound-assisted and enzyme-ultrasound-assisted treatment) and conventional citric acid extraction were applied to obtain pectin from raspberry, blueberry, strawberry and redcurrant, and were compared in terms of extraction yields and physicochemical properties of the extracted pectins.
34237373	1	22	theme	ultrasound-assisted	181:199	arg1	acid					176:179	citric acid ultrasound-assisted	169:199	citric acid ultrasound-assisted	169:199	Three non-conventional extraction techniques (enzyme-assisted with cellulase, citric acid ultrasound-assisted and enzyme-ultrasound-assisted treatment) and conventional citric acid extraction were applied to obtain pectin from raspberry, blueberry, strawberry and redcurrant, and were compared in terms of extraction yields and physicochemical properties of the extracted pectins.
34237373	2	23	theme	acid	526:529	arg1	extraction					531:540	conventional citric acid extraction	506:540	conventional citric acid extraction	506:540	Except for pectin from raspberry, conventional citric acid extraction led to the highest extraction yield (~8%) and, for the same berries, the lowest pectin recovery was found for the extraction with cellulase (~4%).
34237373	1	24	theme	extraction	397:406	arg1	yields					408:413	extraction yields	397:413	extraction yields	397:413	Three non-conventional extraction techniques (enzyme-assisted with cellulase, citric acid ultrasound-assisted and enzyme-ultrasound-assisted treatment) and conventional citric acid extraction were applied to obtain pectin from raspberry, blueberry, strawberry and redcurrant, and were compared in terms of extraction yields and physicochemical properties of the extracted pectins.
34237373	2	25	theme	citric	519:524	arg1	extraction					531:540	conventional citric acid extraction	506:540	conventional citric acid extraction	506:540	Except for pectin from raspberry, conventional citric acid extraction led to the highest extraction yield (~8%) and, for the same berries, the lowest pectin recovery was found for the extraction with cellulase (~4%).
34237373	7	26	theme	efficient	1530:1538	arg1	sources					1540:1546	efficient sources	1530:1546	efficient sources of pectin	1530:1556	Finally, it can be considered that the berries here studied are efficient sources of pectin.
34237373	7	26	theme	efficient	1530:1538	arg1	berries					1505:1511	the berries	1501:1511	the berries here studied	1501:1524	Finally, it can be considered that the berries here studied are efficient sources of pectin.
34237373	3	27	located	found	907:911	arg1	general					874:880	general	874:880	general	874:880	Regarding the structural characteristics of pectins, enzymatically extracted pectins from redcurrant and strawberry exhibited the highest levels of galacturonic acid (≥73%) whereas, in general, this monosaccharide was found from 51 to 69% in the rest of samples.
34237373	3	27	located	found	907:911	arg1	rest					935:938	the rest	931:938	the rest of samples	931:949	Regarding the structural characteristics of pectins, enzymatically extracted pectins from redcurrant and strawberry exhibited the highest levels of galacturonic acid (≥73%) whereas, in general, this monosaccharide was found from 51 to 69% in the rest of samples.
34237373	3	27	located	found	907:911	arg2	monosaccharide					888:901	this monosaccharide	883:901	this monosaccharide	883:901	Regarding the structural characteristics of pectins, enzymatically extracted pectins from redcurrant and strawberry exhibited the highest levels of galacturonic acid (≥73%) whereas, in general, this monosaccharide was found from 51 to 69% in the rest of samples.
34237373	1	28	theme	yields	408:413	arg1	terms					388:392	terms	388:392	terms of extraction yields and physicochemical properties of the extracted pectins	388:469	Three non-conventional extraction techniques (enzyme-assisted with cellulase, citric acid ultrasound-assisted and enzyme-ultrasound-assisted treatment) and conventional citric acid extraction were applied to obtain pectin from raspberry, blueberry, strawberry and redcurrant, and were compared in terms of extraction yields and physicochemical properties of the extracted pectins.
34237373	1	29	from	raspberry	318:326	arg1	pectin					306:311	pectin	306:311	pectin from raspberry, blueberry, strawberry and redcurrant	306:364	Three non-conventional extraction techniques (enzyme-assisted with cellulase, citric acid ultrasound-assisted and enzyme-ultrasound-assisted treatment) and conventional citric acid extraction were applied to obtain pectin from raspberry, blueberry, strawberry and redcurrant, and were compared in terms of extraction yields and physicochemical properties of the extracted pectins.
34237373	1	30	theme	enzyme-ultrasound-assisted	205:230	arg1	treatment					232:240	enzyme-ultrasound-assisted treatment	205:240	enzyme-ultrasound-assisted treatment	205:240	Three non-conventional extraction techniques (enzyme-assisted with cellulase, citric acid ultrasound-assisted and enzyme-ultrasound-assisted treatment) and conventional citric acid extraction were applied to obtain pectin from raspberry, blueberry, strawberry and redcurrant, and were compared in terms of extraction yields and physicochemical properties of the extracted pectins.
34237373	0	31	theme	Berry	0:4	arg1	fruits					6:11	Berry fruits	0:11	Berry fruits as source of pectin: Conventional and non-conventional extraction techniques.	0:89	Berry fruits as source of pectin: Conventional and non-conventional extraction techniques.
34237373	5	32	theme	different	1121:1129	arg1	composition					1141:1151	different monomeric composition	1121:1151	different monomeric composition	1121:1151	The different monomeric composition and the wide range of molecular weight of the obtained pectins pointed out their usefulness in different potential food applications (e.g., thickening, gelling ingredients) and biological activities.
34237373	2	33	theme	lowest	615:620	arg1	recovery					629:636	the lowest pectin recovery	611:636	the lowest pectin recovery	611:636	Except for pectin from raspberry, conventional citric acid extraction led to the highest extraction yield (~8%) and, for the same berries, the lowest pectin recovery was found for the extraction with cellulase (~4%).
34237373	1	34	with	enzyme-assisted	137:151	arg1	treatment					232:240	enzyme-ultrasound-assisted treatment	205:240	enzyme-ultrasound-assisted treatment	205:240	Three non-conventional extraction techniques (enzyme-assisted with cellulase, citric acid ultrasound-assisted and enzyme-ultrasound-assisted treatment) and conventional citric acid extraction were applied to obtain pectin from raspberry, blueberry, strawberry and redcurrant, and were compared in terms of extraction yields and physicochemical properties of the extracted pectins.
34237373	1	34	with	enzyme-assisted	137:151	arg1	cellulase					158:166	cellulase	158:166	cellulase	158:166	Three non-conventional extraction techniques (enzyme-assisted with cellulase, citric acid ultrasound-assisted and enzyme-ultrasound-assisted treatment) and conventional citric acid extraction were applied to obtain pectin from raspberry, blueberry, strawberry and redcurrant, and were compared in terms of extraction yields and physicochemical properties of the extracted pectins.
34237373	1	34	with	enzyme-assisted	137:151	arg1	acid					176:179	citric acid ultrasound-assisted	169:199	citric acid ultrasound-assisted	169:199	Three non-conventional extraction techniques (enzyme-assisted with cellulase, citric acid ultrasound-assisted and enzyme-ultrasound-assisted treatment) and conventional citric acid extraction were applied to obtain pectin from raspberry, blueberry, strawberry and redcurrant, and were compared in terms of extraction yields and physicochemical properties of the extracted pectins.
34237373	4	35	theme	"	1061:1061	arg1	components					1063:1072	"non-pectic" components	1050:1072	"non-pectic" components leading to the obtainment of purer pectin	1050:1114	Although, ultrasound-assisted extraction did not improve pectin yield, it minimized the levels of "non-pectic" components leading to the obtainment of purer pectin.
34237373	4	36	theme	pectin	1009:1014	arg1	yield					1016:1020	pectin yield	1009:1020	pectin yield	1009:1020	Although, ultrasound-assisted extraction did not improve pectin yield, it minimized the levels of "non-pectic" components leading to the obtainment of purer pectin.
34237373	3	37	theme	extracted	756:764	arg1	pectins					766:772	enzymatically extracted pectins	742:772	enzymatically extracted pectins from redcurrant and strawberry	742:803	Regarding the structural characteristics of pectins, enzymatically extracted pectins from redcurrant and strawberry exhibited the highest levels of galacturonic acid (≥73%) whereas, in general, this monosaccharide was found from 51 to 69% in the rest of samples.
34237373	1	38	theme	non-conventional	97:112	arg1	techniques					125:134	Three non-conventional extraction techniques	91:134	Three non-conventional extraction techniques (enzyme-assisted with cellulase, citric acid ultrasound-assisted and enzyme-ultrasound-assisted treatment)	91:241	Three non-conventional extraction techniques (enzyme-assisted with cellulase, citric acid ultrasound-assisted and enzyme-ultrasound-assisted treatment) and conventional citric acid extraction were applied to obtain pectin from raspberry, blueberry, strawberry and redcurrant, and were compared in terms of extraction yields and physicochemical properties of the extracted pectins.
34237373	4	39	theme	non-pectic	1051:1060	arg1	components					1063:1072	"non-pectic" components	1050:1072	"non-pectic" components leading to the obtainment of purer pectin	1050:1114	Although, ultrasound-assisted extraction did not improve pectin yield, it minimized the levels of "non-pectic" components leading to the obtainment of purer pectin.
34237373	3	40	theme	pectins	733:739	arg1	characteristics					714:728	the structural characteristics	699:728	the structural characteristics of pectins	699:739	Regarding the structural characteristics of pectins, enzymatically extracted pectins from redcurrant and strawberry exhibited the highest levels of galacturonic acid (≥73%) whereas, in general, this monosaccharide was found from 51 to 69% in the rest of samples.
34237373	1	41	theme	extraction	114:123	arg1	techniques					125:134	Three non-conventional extraction techniques	91:134	Three non-conventional extraction techniques (enzyme-assisted with cellulase, citric acid ultrasound-assisted and enzyme-ultrasound-assisted treatment)	91:241	Three non-conventional extraction techniques (enzyme-assisted with cellulase, citric acid ultrasound-assisted and enzyme-ultrasound-assisted treatment) and conventional citric acid extraction were applied to obtain pectin from raspberry, blueberry, strawberry and redcurrant, and were compared in terms of extraction yields and physicochemical properties of the extracted pectins.
34237373	5	42	theme	potential	1258:1266	arg1	applications					1273:1284	different potential food applications	1248:1284	different potential food applications (e.g., thickening, gelling ingredients)	1248:1324	The different monomeric composition and the wide range of molecular weight of the obtained pectins pointed out their usefulness in different potential food applications (e.g., thickening, gelling ingredients) and biological activities.
34237373	5	42	theme	potential	1258:1266	arg1	ingredients					1313:1323	gelling ingredients	1305:1323	gelling ingredients	1305:1323	The different monomeric composition and the wide range of molecular weight of the obtained pectins pointed out their usefulness in different potential food applications (e.g., thickening, gelling ingredients) and biological activities.
34237373	0	43	theme	pectin	26:31	arg1	source					16:21	source	16:21	source of pectin	16:31	Berry fruits as source of pectin: Conventional and non-conventional extraction techniques.
34237373	1	44	theme	physicochemical	419:433	arg1	properties					435:444	physicochemical properties	419:444	physicochemical properties of the extracted pectins	419:469	Three non-conventional extraction techniques (enzyme-assisted with cellulase, citric acid ultrasound-assisted and enzyme-ultrasound-assisted treatment) and conventional citric acid extraction were applied to obtain pectin from raspberry, blueberry, strawberry and redcurrant, and were compared in terms of extraction yields and physicochemical properties of the extracted pectins.
34237373	4	45	theme	components	1063:1072	arg1	levels					1040:1045	the levels	1036:1045	the levels of "non-pectic" components leading to the obtainment of purer pectin	1036:1114	Although, ultrasound-assisted extraction did not improve pectin yield, it minimized the levels of "non-pectic" components leading to the obtainment of purer pectin.
34237373	1	46	theme	conventional	247:258	arg1	extraction					272:281	conventional citric acid extraction	247:281	conventional citric acid extraction	247:281	Three non-conventional extraction techniques (enzyme-assisted with cellulase, citric acid ultrasound-assisted and enzyme-ultrasound-assisted treatment) and conventional citric acid extraction were applied to obtain pectin from raspberry, blueberry, strawberry and redcurrant, and were compared in terms of extraction yields and physicochemical properties of the extracted pectins.
34237373	2	47	theme	conventional	506:517	arg1	extraction					531:540	conventional citric acid extraction	506:540	conventional citric acid extraction	506:540	Except for pectin from raspberry, conventional citric acid extraction led to the highest extraction yield (~8%) and, for the same berries, the lowest pectin recovery was found for the extraction with cellulase (~4%).
34237373	5	48	theme	wide	1161:1164	arg1	range					1166:1170	the wide range	1157:1170	the wide range of molecular weight	1157:1190	The different monomeric composition and the wide range of molecular weight of the obtained pectins pointed out their usefulness in different potential food applications (e.g., thickening, gelling ingredients) and biological activities.
34237373	1	49	theme	properties	435:444	arg1	terms					388:392	terms	388:392	terms of extraction yields and physicochemical properties of the extracted pectins	388:469	Three non-conventional extraction techniques (enzyme-assisted with cellulase, citric acid ultrasound-assisted and enzyme-ultrasound-assisted treatment) and conventional citric acid extraction were applied to obtain pectin from raspberry, blueberry, strawberry and redcurrant, and were compared in terms of extraction yields and physicochemical properties of the extracted pectins.
34237373	5	50	theme	biological	1330:1339	arg1	activities					1341:1350	biological activities	1330:1350	biological activities	1330:1350	The different monomeric composition and the wide range of molecular weight of the obtained pectins pointed out their usefulness in different potential food applications (e.g., thickening, gelling ingredients) and biological activities.
34237373	1	51	theme	citric	260:265	arg1	extraction					272:281	conventional citric acid extraction	247:281	conventional citric acid extraction	247:281	Three non-conventional extraction techniques (enzyme-assisted with cellulase, citric acid ultrasound-assisted and enzyme-ultrasound-assisted treatment) and conventional citric acid extraction were applied to obtain pectin from raspberry, blueberry, strawberry and redcurrant, and were compared in terms of extraction yields and physicochemical properties of the extracted pectins.
34237373	0	52	theme	Conventional	34:45	arg1	techniques					79:88	Conventional and non-conventional extraction techniques	34:88	Berry fruits as source of pectin: Conventional and non-conventional extraction techniques.	0:89	Berry fruits as source of pectin: Conventional and non-conventional extraction techniques.
34237373	3	53	from	strawberry	794:803	arg1	pectins					766:772	enzymatically extracted pectins	742:772	enzymatically extracted pectins from redcurrant and strawberry	742:803	Regarding the structural characteristics of pectins, enzymatically extracted pectins from redcurrant and strawberry exhibited the highest levels of galacturonic acid (≥73%) whereas, in general, this monosaccharide was found from 51 to 69% in the rest of samples.
34237373	5	54	theme	monomeric	1131:1139	arg1	composition					1141:1151	different monomeric composition	1121:1151	different monomeric composition	1121:1151	The different monomeric composition and the wide range of molecular weight of the obtained pectins pointed out their usefulness in different potential food applications (e.g., thickening, gelling ingredients) and biological activities.
34237373	4	55	theme	ultrasound-assisted	962:980	arg1	extraction					982:991	ultrasound-assisted extraction	962:991	ultrasound-assisted extraction	962:991	Although, ultrasound-assisted extraction did not improve pectin yield, it minimized the levels of "non-pectic" components leading to the obtainment of purer pectin.
34237373	3	56	from	redcurrant	779:788	arg1	pectins					766:772	enzymatically extracted pectins	742:772	enzymatically extracted pectins from redcurrant and strawberry	742:803	Regarding the structural characteristics of pectins, enzymatically extracted pectins from redcurrant and strawberry exhibited the highest levels of galacturonic acid (≥73%) whereas, in general, this monosaccharide was found from 51 to 69% in the rest of samples.
34237373	5	57	theme	weight	1185:1190	arg1	range					1166:1170	the wide range	1157:1170	the wide range of molecular weight	1157:1190	The different monomeric composition and the wide range of molecular weight of the obtained pectins pointed out their usefulness in different potential food applications (e.g., thickening, gelling ingredients) and biological activities.
34237373	5	57	theme	weight	1185:1190	arg1	composition					1141:1151	different monomeric composition	1121:1151	different monomeric composition	1121:1151	The different monomeric composition and the wide range of molecular weight of the obtained pectins pointed out their usefulness in different potential food applications (e.g., thickening, gelling ingredients) and biological activities.
34237373	1	58	theme	extracted	453:461	arg1	pectins					463:469	the extracted pectins	449:469	the extracted pectins	449:469	Three non-conventional extraction techniques (enzyme-assisted with cellulase, citric acid ultrasound-assisted and enzyme-ultrasound-assisted treatment) and conventional citric acid extraction were applied to obtain pectin from raspberry, blueberry, strawberry and redcurrant, and were compared in terms of extraction yields and physicochemical properties of the extracted pectins.
34237373	1	59	theme	acid	267:270	arg1	extraction					272:281	conventional citric acid extraction	247:281	conventional citric acid extraction	247:281	Three non-conventional extraction techniques (enzyme-assisted with cellulase, citric acid ultrasound-assisted and enzyme-ultrasound-assisted treatment) and conventional citric acid extraction were applied to obtain pectin from raspberry, blueberry, strawberry and redcurrant, and were compared in terms of extraction yields and physicochemical properties of the extracted pectins.
34237373	1	60	theme	pectins	463:469	arg1	properties					435:444	physicochemical properties	419:444	physicochemical properties of the extracted pectins	419:469	Three non-conventional extraction techniques (enzyme-assisted with cellulase, citric acid ultrasound-assisted and enzyme-ultrasound-assisted treatment) and conventional citric acid extraction were applied to obtain pectin from raspberry, blueberry, strawberry and redcurrant, and were compared in terms of extraction yields and physicochemical properties of the extracted pectins.
34237373	1	60	theme	pectins	463:469	arg1	yields					408:413	extraction yields	397:413	extraction yields	397:413	Three non-conventional extraction techniques (enzyme-assisted with cellulase, citric acid ultrasound-assisted and enzyme-ultrasound-assisted treatment) and conventional citric acid extraction were applied to obtain pectin from raspberry, blueberry, strawberry and redcurrant, and were compared in terms of extraction yields and physicochemical properties of the extracted pectins.
34237373	5	61	dep	thickening	1293:1302	arg1	e.g.					1287:1290	e.g.	1287:1290	e.g.	1287:1290	The different monomeric composition and the wide range of molecular weight of the obtained pectins pointed out their usefulness in different potential food applications (e.g., thickening, gelling ingredients) and biological activities.
34237373	3	62	theme	samples	943:949	arg1	rest					935:938	the rest	931:938	the rest of samples	931:949	Regarding the structural characteristics of pectins, enzymatically extracted pectins from redcurrant and strawberry exhibited the highest levels of galacturonic acid (≥73%) whereas, in general, this monosaccharide was found from 51 to 69% in the rest of samples.
34237373	5	63	theme	food	1268:1271	arg1	applications					1273:1284	different potential food applications	1248:1284	different potential food applications (e.g., thickening, gelling ingredients)	1248:1324	The different monomeric composition and the wide range of molecular weight of the obtained pectins pointed out their usefulness in different potential food applications (e.g., thickening, gelling ingredients) and biological activities.
34237373	5	63	theme	food	1268:1271	arg1	ingredients					1313:1323	gelling ingredients	1305:1323	gelling ingredients	1305:1323	The different monomeric composition and the wide range of molecular weight of the obtained pectins pointed out their usefulness in different potential food applications (e.g., thickening, gelling ingredients) and biological activities.
34237373	3	64	theme	highest	819:825	arg1	levels					827:832	the highest levels	815:832	the highest levels of galacturonic acid (≥73%)	815:860	Regarding the structural characteristics of pectins, enzymatically extracted pectins from redcurrant and strawberry exhibited the highest levels of galacturonic acid (≥73%) whereas, in general, this monosaccharide was found from 51 to 69% in the rest of samples.
34237373	0	65	theme	non-conventional	51:66	arg1	techniques					79:88	Conventional and non-conventional extraction techniques	34:88	Berry fruits as source of pectin: Conventional and non-conventional extraction techniques.	0:89	Berry fruits as source of pectin: Conventional and non-conventional extraction techniques.
34237373	0	66	dep	fruits	6:11	arg1	techniques					79:88	Conventional and non-conventional extraction techniques	34:88	Berry fruits as source of pectin: Conventional and non-conventional extraction techniques.	0:89	Berry fruits as source of pectin: Conventional and non-conventional extraction techniques.
34237373	4	67	theme	purer	1103:1107	arg1	pectin					1109:1114	purer pectin	1103:1114	purer pectin	1103:1114	Although, ultrasound-assisted extraction did not improve pectin yield, it minimized the levels of "non-pectic" components leading to the obtainment of purer pectin.
34237373	5	68	theme	different	1248:1256	arg1	applications					1273:1284	different potential food applications	1248:1284	different potential food applications (e.g., thickening, gelling ingredients)	1248:1324	The different monomeric composition and the wide range of molecular weight of the obtained pectins pointed out their usefulness in different potential food applications (e.g., thickening, gelling ingredients) and biological activities.
34237373	5	68	theme	different	1248:1256	arg1	ingredients					1313:1323	gelling ingredients	1305:1323	gelling ingredients	1305:1323	The different monomeric composition and the wide range of molecular weight of the obtained pectins pointed out their usefulness in different potential food applications (e.g., thickening, gelling ingredients) and biological activities.
34237373	6	69	theme	techno-functional	1431:1447	arg1	characteristics					1449:1463	their physicochemical and techno-functional characteristics	1405:1463	their physicochemical and techno-functional characteristics	1405:1463	This has been evidenced by the differences found in their physicochemical and techno-functional characteristics.
34237373	5	70	dep	composition	1141:1151	arg1	The					1117:1119	The	1117:1119	The	1117:1119	The different monomeric composition and the wide range of molecular weight of the obtained pectins pointed out their usefulness in different potential food applications (e.g., thickening, gelling ingredients) and biological activities.
34237373	5	71	theme	molecular	1175:1183	arg1	weight					1185:1190	molecular weight	1175:1190	molecular weight	1175:1190	The different monomeric composition and the wide range of molecular weight of the obtained pectins pointed out their usefulness in different potential food applications (e.g., thickening, gelling ingredients) and biological activities.
34237373	5	72	theme	obtained	1199:1206	arg1	pectins					1208:1214	the obtained pectins	1195:1214	the obtained pectins	1195:1214	The different monomeric composition and the wide range of molecular weight of the obtained pectins pointed out their usefulness in different potential food applications (e.g., thickening, gelling ingredients) and biological activities.
34237373	7	73	theme	pectin	1551:1556	arg1	sources					1540:1546	efficient sources	1530:1546	efficient sources of pectin	1530:1556	Finally, it can be considered that the berries here studied are efficient sources of pectin.
34237373	7	73	theme	pectin	1551:1556	arg1	berries					1505:1511	the berries	1501:1511	the berries here studied	1501:1524	Finally, it can be considered that the berries here studied are efficient sources of pectin.
34199374	2	0	theme	volumetric	506:515	arg1	action					517:522	the conventional volumetric action	489:522	the conventional volumetric action	489:522	Different from originally developed gels, they are intended for more superficial injections, claiming a skin rejuvenation effect through hydration and possibly prompting biochemical effects in place of the conventional volumetric action.
34199374	9	1	theme	biochemical	1361:1371	arg1	parameters					1373:1382	biophysical and biochemical parameters	1345:1382	biophysical and biochemical parameters for skinboosters	1345:1399	CONCLUSIONS A wide panel of biophysical and biochemical parameters for skinboosters was provided, supporting clinicians in the conscious tuning of their use.
34199374	7	2	theme	stability	1197:1205	arg1	ranking					1207:1213	a stability ranking	1195:1213	a stability ranking	1195:1213	Sensitivity to hyaluronidases and Reactive Oxygen Species was demonstrated allowing a stability ranking.
34199374	2	3	theme	superficial	356:366	arg1	injections					368:377	more superficial injections	351:377	more superficial injections	351:377	Different from originally developed gels, they are intended for more superficial injections, claiming a skin rejuvenation effect through hydration and possibly prompting biochemical effects in place of the conventional volumetric action.
34199374	1	4	theme	hydrogels	244:252	arg1	category					216:223	the latest category	205:223	the latest category of hyaluronan (HA) hydrogels released for aesthetic purposes	205:284	BACKGROUND Skinboosters represent the latest category of hyaluronan (HA) hydrogels released for aesthetic purposes.
34199374	0	5	theme	In	68:69	arg1	Characterization					77:92	An In Vitro Characterization	65:92	Hyaluronan Hydrogels for Injection in Superficial Dermal Layers: An In Vitro Characterization to Compare Performance and Unravel the Scientific Basis of Their Indication.	0:169	Hyaluronan Hydrogels for Injection in Superficial Dermal Layers: An In Vitro Characterization to Compare Performance and Unravel the Scientific Basis of Their Indication.
34199374	0	6	from	Hydrogels	11:19	arg1	Layers					57:62	Superficial Dermal Layers	38:62	Superficial Dermal Layers	38:62	Hyaluronan Hydrogels for Injection in Superficial Dermal Layers: An In Vitro Characterization to Compare Performance and Unravel the Scientific Basis of Their Indication.
34199374	10	7	theme	clinical	1550:1557	arg1	indication					1559:1568	the same clinical indication and unexpected similarities	1541:1596	indication	1559:1568	Data revealed great variability in gels' behavior notwithstanding the same clinical indication and unexpected similarities to the volumetric formulations.
34199374	2	8	from	gels	323:326	arg1	Different					287:295	Different	287:295	Different	287:295	Different from originally developed gels, they are intended for more superficial injections, claiming a skin rejuvenation effect through hydration and possibly prompting biochemical effects in place of the conventional volumetric action.
34199374	0	9	theme	Hyaluronan	0:9	arg1	Hydrogels					11:19	Hyaluronan Hydrogels	0:19	Hyaluronan Hydrogels for Injection in Superficial Dermal Layers: An In Vitro Characterization to Compare Performance and Unravel the Scientific Basis of Their Indication.	0:169	Hyaluronan Hydrogels for Injection in Superficial Dermal Layers: An In Vitro Characterization to Compare Performance and Unravel the Scientific Basis of Their Indication.
34199374	1	10	theme	aesthetic	267:275	arg1	purposes					277:284	aesthetic purposes	267:284	aesthetic purposes	267:284	BACKGROUND Skinboosters represent the latest category of hyaluronan (HA) hydrogels released for aesthetic purposes.
34199374	4	11	theme	human	807:811	arg1	fibroblasts					820:830	human dermal fibroblasts	807:830	human dermal fibroblasts towards the production of extracellular matrix components	807:888	METHODS Gels were evaluated for water-soluble/insoluble-HA composition, rheology, hydration, cohesivity, stability and effect, in vitro, on human dermal fibroblasts towards the production of extracellular matrix components.
34199374	2	12	theme	rejuvenation	396:407	arg1	effect					409:414	a skin rejuvenation effect	389:414	a skin rejuvenation effect	389:414	Different from originally developed gels, they are intended for more superficial injections, claiming a skin rejuvenation effect through hydration and possibly prompting biochemical effects in place of the conventional volumetric action.
34199374	4	13	theme	components	879:888	arg1	production					844:853	the production	840:853	the production of extracellular matrix components	840:888	METHODS Gels were evaluated for water-soluble/insoluble-HA composition, rheology, hydration, cohesivity, stability and effect, in vitro, on human dermal fibroblasts towards the production of extracellular matrix components.
34199374	0	14	dep	Characterization	77:92	arg1	Compare					97:103	Compare	97:103	Compare Performance	97:115	Hyaluronan Hydrogels for Injection in Superficial Dermal Layers: An In Vitro Characterization to Compare Performance and Unravel the Scientific Basis of Their Indication.
34199374	0	14	dep	Characterization	77:92	arg1	Unravel					121:127	Unravel	121:127	Unravel the Scientific Basis of Their Indication	121:168	Hyaluronan Hydrogels for Injection in Superficial Dermal Layers: An In Vitro Characterization to Compare Performance and Unravel the Scientific Basis of Their Indication.
34199374	11	15	dep	specific	1695:1702	arg1	uses					1704:1707	uses	1704:1707	uses	1704:1707	Data may be useful to improve customization of gel design toward specific uses.
34199374	1	16	theme	BACKGROUND	171:180	arg1	Skinboosters					182:193	BACKGROUND Skinboosters	171:193	BACKGROUND Skinboosters	171:193	BACKGROUND Skinboosters represent the latest category of hyaluronan (HA) hydrogels released for aesthetic purposes.
34199374	2	17	theme	conventional	493:504	arg1	action					517:522	the conventional volumetric action	489:522	the conventional volumetric action	489:522	Different from originally developed gels, they are intended for more superficial injections, claiming a skin rejuvenation effect through hydration and possibly prompting biochemical effects in place of the conventional volumetric action.
34199374	7	18	theme	Oxygen	1154:1159	arg1	Species					1161:1167	Reactive Oxygen Species	1145:1167	Reactive Oxygen Species	1145:1167	Sensitivity to hyaluronidases and Reactive Oxygen Species was demonstrated allowing a stability ranking.
34199374	11	19	theme	gel	1677:1679	arg1	design					1681:1686	gel design	1677:1686	gel design	1677:1686	Data may be useful to improve customization of gel design toward specific uses.
34199374	3	20	theme	scientific	595:604	arg1	basis					606:610	the scientific basis	591:610	the scientific basis for such indication	591:630	Here, three commercial skinboosters were characterized to unravel the scientific basis for such indication and to compare their performances.
34199374	2	21	theme	biochemical	457:467	arg1	effects					469:475	biochemical effects	457:475	biochemical effects	457:475	Different from originally developed gels, they are intended for more superficial injections, claiming a skin rejuvenation effect through hydration and possibly prompting biochemical effects in place of the conventional volumetric action.
34199374	9	22	theme	biophysical	1345:1355	arg1	parameters					1373:1382	biophysical and biochemical parameters	1345:1382	biophysical and biochemical parameters for skinboosters	1345:1399	CONCLUSIONS A wide panel of biophysical and biochemical parameters for skinboosters was provided, supporting clinicians in the conscious tuning of their use.
34199374	0	23	dep	In	68:69	arg1	Vitro					71:75	Vitro	71:75	Vitro	71:75	Hyaluronan Hydrogels for Injection in Superficial Dermal Layers: An In Vitro Characterization to Compare Performance and Unravel the Scientific Basis of Their Indication.
34199374	4	24	theme	water-soluble/insoluble-HA	699:724	arg1	cohesivity					760:769	cohesivity	760:769	cohesivity	760:769	METHODS Gels were evaluated for water-soluble/insoluble-HA composition, rheology, hydration, cohesivity, stability and effect, in vitro, on human dermal fibroblasts towards the production of extracellular matrix components.
34199374	4	24	theme	water-soluble/insoluble-HA	699:724	arg1	stability					772:780	stability	772:780	stability	772:780	METHODS Gels were evaluated for water-soluble/insoluble-HA composition, rheology, hydration, cohesivity, stability and effect, in vitro, on human dermal fibroblasts towards the production of extracellular matrix components.
34199374	4	24	theme	water-soluble/insoluble-HA	699:724	arg1	rheology					739:746	rheology	739:746	rheology	739:746	METHODS Gels were evaluated for water-soluble/insoluble-HA composition, rheology, hydration, cohesivity, stability and effect, in vitro, on human dermal fibroblasts towards the production of extracellular matrix components.
34199374	4	24	theme	water-soluble/insoluble-HA	699:724	arg1	hydration					749:757	hydration	749:757	hydration	749:757	METHODS Gels were evaluated for water-soluble/insoluble-HA composition, rheology, hydration, cohesivity, stability and effect, in vitro, on human dermal fibroblasts towards the production of extracellular matrix components.
34199374	4	24	theme	water-soluble/insoluble-HA	699:724	arg1	effect					786:791	effect	786:791	effect	786:791	METHODS Gels were evaluated for water-soluble/insoluble-HA composition, rheology, hydration, cohesivity, stability and effect, in vitro, on human dermal fibroblasts towards the production of extracellular matrix components.
34199374	4	24	theme	water-soluble/insoluble-HA	699:724	arg1	composition					726:736	water-soluble/insoluble-HA composition	699:736	water-soluble/insoluble-HA composition	699:736	METHODS Gels were evaluated for water-soluble/insoluble-HA composition, rheology, hydration, cohesivity, stability and effect, in vitro, on human dermal fibroblasts towards the production of extracellular matrix components.
34199374	8	25	theme	elastin	1273:1279	arg1	expression					1281:1290	elastin expression	1273:1290	elastin expression	1273:1290	Slight differences were found in gels' ability to prompt elastin expression and in ColIV/ColI ratio.
34199374	4	26	theme	extracellular	858:870	arg1	components					879:888	extracellular matrix components	858:888	extracellular matrix components	858:888	METHODS Gels were evaluated for water-soluble/insoluble-HA composition, rheology, hydration, cohesivity, stability and effect, in vitro, on human dermal fibroblasts towards the production of extracellular matrix components.
34199374	2	27	theme	developed	313:321	arg1	gels					323:326	originally developed gels	302:326	originally developed gels	302:326	Different from originally developed gels, they are intended for more superficial injections, claiming a skin rejuvenation effect through hydration and possibly prompting biochemical effects in place of the conventional volumetric action.
34199374	10	28	theme	same	1545:1548	arg1	indication					1559:1568	the same clinical indication and unexpected similarities	1541:1596	indication	1559:1568	Data revealed great variability in gels' behavior notwithstanding the same clinical indication and unexpected similarities to the volumetric formulations.
34199374	4	29	theme	METHODS	667:673	arg1	Gels					675:678	METHODS Gels	667:678	METHODS Gels	667:678	METHODS Gels were evaluated for water-soluble/insoluble-HA composition, rheology, hydration, cohesivity, stability and effect, in vitro, on human dermal fibroblasts towards the production of extracellular matrix components.
34199374	7	30	theme	Reactive	1145:1152	arg1	Species					1161:1167	Reactive Oxygen Species	1145:1167	Reactive Oxygen Species	1145:1167	Sensitivity to hyaluronidases and Reactive Oxygen Species was demonstrated allowing a stability ranking.
34199374	1	31	theme	latest	209:214	arg1	category					216:223	the latest category	205:223	the latest category of hyaluronan (HA) hydrogels released for aesthetic purposes	205:284	BACKGROUND Skinboosters represent the latest category of hyaluronan (HA) hydrogels released for aesthetic purposes.
34199374	0	32	theme	Superficial	38:48	arg1	Layers					57:62	Superficial Dermal Layers	38:62	Superficial Dermal Layers	38:62	Hyaluronan Hydrogels for Injection in Superficial Dermal Layers: An In Vitro Characterization to Compare Performance and Unravel the Scientific Basis of Their Indication.
34199374	0	33	theme	Scientific	133:142	arg1	Basis					144:148	the Scientific Basis	129:148	the Scientific Basis of Their Indication	129:168	Hyaluronan Hydrogels for Injection in Superficial Dermal Layers: An In Vitro Characterization to Compare Performance and Unravel the Scientific Basis of Their Indication.
34199374	5	34	theme	insoluble-hydrogel	925:942	arg1	amount					944:949	the insoluble-hydrogel amount	921:949	the insoluble-hydrogel amount	921:949	RESULTS Marked differences in the insoluble-hydrogel amount and in the hydrodynamic parameters for water-soluble-HA chains were evidenced among the gels.
34199374	9	35	theme	conscious	1444:1452	arg1	tuning					1454:1459	the conscious tuning	1440:1459	the conscious tuning of their use	1440:1472	CONCLUSIONS A wide panel of biophysical and biochemical parameters for skinboosters was provided, supporting clinicians in the conscious tuning of their use.
34199374	10	36	theme	unexpected	1574:1583	arg1	similarities					1585:1596	the same clinical indication and unexpected similarities	1541:1596	similarities	1585:1596	Data revealed great variability in gels' behavior notwithstanding the same clinical indication and unexpected similarities to the volumetric formulations.
34199374	9	37	dep	CONCLUSIONS	1317:1327	arg1	provided					1405:1412	provided	1405:1412	provided	1405:1412	CONCLUSIONS A wide panel of biophysical and biochemical parameters for skinboosters was provided, supporting clinicians in the conscious tuning of their use.
34199374	5	38	theme	Marked	899:904	arg1	differences					906:916	Marked differences	899:916	Marked differences in the insoluble-hydrogel amount and in the hydrodynamic parameters for water-soluble-HA chains	899:1012	RESULTS Marked differences in the insoluble-hydrogel amount and in the hydrodynamic parameters for water-soluble-HA chains were evidenced among the gels.
34199374	8	39	theme	ColIV/ColI	1299:1308	arg1	ratio					1310:1314	ColIV/ColI ratio	1299:1314	ColIV/ColI ratio	1299:1314	Slight differences were found in gels' ability to prompt elastin expression and in ColIV/ColI ratio.
34199374	4	40	theme	matrix	872:877	arg1	components					879:888	extracellular matrix components	858:888	extracellular matrix components	858:888	METHODS Gels were evaluated for water-soluble/insoluble-HA composition, rheology, hydration, cohesivity, stability and effect, in vitro, on human dermal fibroblasts towards the production of extracellular matrix components.
34199374	5	41	theme	hydrodynamic	962:973	arg1	parameters					975:984	the hydrodynamic parameters	958:984	the hydrodynamic parameters for water-soluble-HA chains	958:1012	RESULTS Marked differences in the insoluble-hydrogel amount and in the hydrodynamic parameters for water-soluble-HA chains were evidenced among the gels.
34199374	0	42	theme	Dermal	50:55	arg1	Layers					57:62	Superficial Dermal Layers	38:62	Superficial Dermal Layers	38:62	Hyaluronan Hydrogels for Injection in Superficial Dermal Layers: An In Vitro Characterization to Compare Performance and Unravel the Scientific Basis of Their Indication.
34199374	9	43	theme	wide	1331:1334	arg1	panel					1336:1340	A wide panel	1329:1340	A wide panel of biophysical and biochemical parameters for skinboosters	1329:1399	CONCLUSIONS A wide panel of biophysical and biochemical parameters for skinboosters was provided, supporting clinicians in the conscious tuning of their use.
34199374	3	44	theme	such	616:619	arg1	indication					621:630	such indication	616:630	such indication	616:630	Here, three commercial skinboosters were characterized to unravel the scientific basis for such indication and to compare their performances.
34199374	11	45	theme	design	1681:1686	arg1	customization					1660:1672	customization	1660:1672	customization of gel design toward specific uses	1660:1707	Data may be useful to improve customization of gel design toward specific uses.
34199374	10	46	theme	great	1489:1493	arg1	variability					1495:1505	great variability	1489:1505	great variability in gels' behavior notwithstanding the same clinical indication and unexpected similarities to the volumetric formulations	1489:1627	Data revealed great variability in gels' behavior notwithstanding the same clinical indication and unexpected similarities to the volumetric formulations.
34199374	0	47	theme	Indication	159:168	arg1	Basis					144:148	the Scientific Basis	129:148	the Scientific Basis of Their Indication	129:168	Hyaluronan Hydrogels for Injection in Superficial Dermal Layers: An In Vitro Characterization to Compare Performance and Unravel the Scientific Basis of Their Indication.
34199374	5	48	dep	RESULTS	891:897	arg1	evidenced					1019:1027	evidenced	1019:1027	were evidenced among the gels	1014:1042	RESULTS Marked differences in the insoluble-hydrogel amount and in the hydrodynamic parameters for water-soluble-HA chains were evidenced among the gels.
34199374	9	49	theme	use	1470:1472	arg1	tuning					1454:1459	the conscious tuning	1440:1459	the conscious tuning of their use	1440:1472	CONCLUSIONS A wide panel of biophysical and biochemical parameters for skinboosters was provided, supporting clinicians in the conscious tuning of their use.
34199374	1	50	theme	hyaluronan	228:237	arg1	hydrogels					244:252	hyaluronan (HA) hydrogels	228:252	hyaluronan (HA) hydrogels released for aesthetic purposes	228:284	BACKGROUND Skinboosters represent the latest category of hyaluronan (HA) hydrogels released for aesthetic purposes.
34199374	10	51	theme	volumetric	1605:1614	arg1	formulations					1616:1627	the volumetric formulations	1601:1627	the volumetric formulations	1601:1627	Data revealed great variability in gels' behavior notwithstanding the same clinical indication and unexpected similarities to the volumetric formulations.
34199374	4	52	theme	dermal	813:818	arg1	fibroblasts					820:830	human dermal fibroblasts	807:830	human dermal fibroblasts towards the production of extracellular matrix components	807:888	METHODS Gels were evaluated for water-soluble/insoluble-HA composition, rheology, hydration, cohesivity, stability and effect, in vitro, on human dermal fibroblasts towards the production of extracellular matrix components.
34199374	5	53	theme	water-soluble-HA	990:1005	arg1	chains					1007:1012	water-soluble-HA chains	990:1012	water-soluble-HA chains	990:1012	RESULTS Marked differences in the insoluble-hydrogel amount and in the hydrodynamic parameters for water-soluble-HA chains were evidenced among the gels.
34199374	8	54	theme	Slight	1216:1221	arg1	differences					1223:1233	Slight differences	1216:1233	Slight differences	1216:1233	Slight differences were found in gels' ability to prompt elastin expression and in ColIV/ColI ratio.
34199374	6	55	theme	wide	1099:1102	arg1	range					1104:1108	a wide range	1097:1108	a wide range	1097:1108	Hydration, rigidity and cohesivity also varied over a wide range.
34199374	0	56	dep	Hydrogels	11:19	arg1	Characterization					77:92	An In Vitro Characterization	65:92	Hyaluronan Hydrogels for Injection in Superficial Dermal Layers: An In Vitro Characterization to Compare Performance and Unravel the Scientific Basis of Their Indication.	0:169	Hyaluronan Hydrogels for Injection in Superficial Dermal Layers: An In Vitro Characterization to Compare Performance and Unravel the Scientific Basis of Their Indication.
34199374	2	57	theme	skin	391:394	arg1	effect					409:414	a skin rejuvenation effect	389:414	a skin rejuvenation effect	389:414	Different from originally developed gels, they are intended for more superficial injections, claiming a skin rejuvenation effect through hydration and possibly prompting biochemical effects in place of the conventional volumetric action.
34199374	9	58	theme	parameters	1373:1382	arg1	panel					1336:1340	A wide panel	1329:1340	A wide panel of biophysical and biochemical parameters for skinboosters	1329:1399	CONCLUSIONS A wide panel of biophysical and biochemical parameters for skinboosters was provided, supporting clinicians in the conscious tuning of their use.
34199374	5	59	from	differences	906:916	arg1	parameters					975:984	the hydrodynamic parameters	958:984	the hydrodynamic parameters for water-soluble-HA chains	958:1012	RESULTS Marked differences in the insoluble-hydrogel amount and in the hydrodynamic parameters for water-soluble-HA chains were evidenced among the gels.
34199374	5	59	from	differences	906:916	arg1	amount					944:949	the insoluble-hydrogel amount	921:949	the insoluble-hydrogel amount	921:949	RESULTS Marked differences in the insoluble-hydrogel amount and in the hydrodynamic parameters for water-soluble-HA chains were evidenced among the gels.
34199374	3	60	theme	commercial	537:546	arg1	skinboosters					548:559	three commercial skinboosters	531:559	three commercial skinboosters	531:559	Here, three commercial skinboosters were characterized to unravel the scientific basis for such indication and to compare their performances.
32006585	2	0	dep	thermogravimetric	513:529	arg1	TG					532:533	TG	532:533	TG	532:533	The physicochemical properties of the resultant adsorbent were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), vibrating sample magnetometer (VSM) thermogravimetric (TG) analysis, scanning electron microscopy (SEM) and N2 adsorption-desorption.
32006585	1	1	theme	@	297:297	arg1	composite					280:288	chitosan-based magnetic composite	256:288	chitosan-based magnetic composite (CTS-Cu@SiO2@Fe3O4)	256:308	In order to effectively remove reactive brilliant red (RBR) in aqueous solution, a novel Cu(II) coordinated chitosan-based magnetic composite (CTS-Cu@SiO2@Fe3O4) was prepared.
32006585	1	1	theme	@	297:297	arg1	Fe3O4					303:307	CTS-Cu@SiO2@Fe3O4	291:307	CTS-Cu@SiO2@Fe3O4	291:307	In order to effectively remove reactive brilliant red (RBR) in aqueous solution, a novel Cu(II) coordinated chitosan-based magnetic composite (CTS-Cu@SiO2@Fe3O4) was prepared.
32006585	2	2	dep	infrared	422:429	arg1	FTIR					432:435	FTIR	432:435	FTIR	432:435	The physicochemical properties of the resultant adsorbent were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), vibrating sample magnetometer (VSM) thermogravimetric (TG) analysis, scanning electron microscopy (SEM) and N2 adsorption-desorption.
32006585	5	3	theme	CTS-Cu	1036:1041	arg1	Fe3O4					1048:1052	CTS-Cu@SiO2@Fe3O4	1036:1052	CTS-Cu@SiO2@Fe3O4	1036:1052	The adsorption kinetics of CTS-Cu@SiO2@Fe3O4 was found to follow the pseudo-second-order kinetic model, and the equilibrium adsorption data were well described by the Freundlich isotherm model.
32006585	4	4	theme	CTS-Cu	807:812	arg1	Fe3O4					819:823	CTS-Cu@SiO2@Fe3O4	807:823	CTS-Cu@SiO2@Fe3O4	807:823	Compared with CTS@SiO2@Fe3O4, CTS-Cu@SiO2@Fe3O4 showed better adsorption performance in removing RBR, reaching a maximum of 880.84 mg/g at pH 4, which confirmed that the coordination of Cu(II) can improve the adsorption capability.
32006585	4	5	theme	SiO2	795:798	arg1	Fe3O4					800:804	CTS@SiO2@Fe3O4	791:804	CTS@SiO2@Fe3O4	791:804	Compared with CTS@SiO2@Fe3O4, CTS-Cu@SiO2@Fe3O4 showed better adsorption performance in removing RBR, reaching a maximum of 880.84 mg/g at pH 4, which confirmed that the coordination of Cu(II) can improve the adsorption capability.
32006585	2	6	theme	vibrating	477:485	arg1	analysis					536:543	vibrating sample magnetometer (VSM) thermogravimetric (TG) analysis	477:543	vibrating sample magnetometer (VSM) thermogravimetric (TG) analysis	477:543	The physicochemical properties of the resultant adsorbent were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), vibrating sample magnetometer (VSM) thermogravimetric (TG) analysis, scanning electron microscopy (SEM) and N2 adsorption-desorption.
32006585	1	7	theme	SiO2	298:301	arg1	composite					280:288	chitosan-based magnetic composite	256:288	chitosan-based magnetic composite (CTS-Cu@SiO2@Fe3O4)	256:308	In order to effectively remove reactive brilliant red (RBR) in aqueous solution, a novel Cu(II) coordinated chitosan-based magnetic composite (CTS-Cu@SiO2@Fe3O4) was prepared.
32006585	1	7	theme	SiO2	298:301	arg1	Fe3O4					303:307	CTS-Cu@SiO2@Fe3O4	291:307	CTS-Cu@SiO2@Fe3O4	291:307	In order to effectively remove reactive brilliant red (RBR) in aqueous solution, a novel Cu(II) coordinated chitosan-based magnetic composite (CTS-Cu@SiO2@Fe3O4) was prepared.
32006585	2	8	dep	Fourier	404:410	arg1	transform					412:420	transform	412:420	transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), vibrating sample magnetometer (VSM) thermogravimetric (TG) analysis, scanning electron microscopy (SEM) and N2 adsorption-desorption	412:608	The physicochemical properties of the resultant adsorbent were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), vibrating sample magnetometer (VSM) thermogravimetric (TG) analysis, scanning electron microscopy (SEM) and N2 adsorption-desorption.
32006585	8	9	theme	CTS-Cu	1489:1494	arg1	Fe3O4					1501:1505	CTS-Cu@SiO2@Fe3O4	1489:1505	CTS-Cu@SiO2@Fe3O4	1489:1505	Furthermore, regeneration experiments demonstrated that CTS-Cu@SiO2@Fe3O4 can be used repeatedly.
32006585	9	10	theme	SiO2	1549:1552	arg1	Fe3O4					1554:1558	CTS-Cu@SiO2@Fe3O4	1542:1558	CTS-Cu@SiO2@Fe3O4	1542:1558	In a word, CTS-Cu@SiO2@Fe3O4 can be served as a promising adsorbent for dye wastewater scavenging.
32006585	5	11	theme	equilibrium	1121:1131	arg1	data					1144:1147	the equilibrium adsorption data	1117:1147	the equilibrium adsorption data	1117:1147	The adsorption kinetics of CTS-Cu@SiO2@Fe3O4 was found to follow the pseudo-second-order kinetic model, and the equilibrium adsorption data were well described by the Freundlich isotherm model.
32006585	3	12	theme	temperature	764:774	arg1	function					683:690	a function	681:690	a function of contact time, solution pH, initial concentration, ionic strength and temperature	681:774	The adsorption capacity toward RBR was systematically investigated as a function of contact time, solution pH, initial concentration, ionic strength and temperature.
32006585	3	12	theme	temperature	764:774	arg1	capacity					626:633	The adsorption capacity	611:633	The adsorption capacity toward RBR	611:644	The adsorption capacity toward RBR was systematically investigated as a function of contact time, solution pH, initial concentration, ionic strength and temperature.
32006585	6	13	theme	adsorption	1244:1253	arg1	spontaneous					1267:1277	spontaneous	1267:1277	spontaneous	1267:1277	Thermodynamic studies indicated that the adsorption process was spontaneous and endothermic.
32006585	6	13	theme	adsorption	1244:1253	arg1	process					1255:1261	the adsorption process	1240:1261	the adsorption process	1240:1261	Thermodynamic studies indicated that the adsorption process was spontaneous and endothermic.
32006585	1	14	theme	@	302:302	arg1	composite					280:288	chitosan-based magnetic composite	256:288	chitosan-based magnetic composite (CTS-Cu@SiO2@Fe3O4)	256:308	In order to effectively remove reactive brilliant red (RBR) in aqueous solution, a novel Cu(II) coordinated chitosan-based magnetic composite (CTS-Cu@SiO2@Fe3O4) was prepared.
32006585	1	14	theme	@	302:302	arg1	Fe3O4					303:307	CTS-Cu@SiO2@Fe3O4	291:307	CTS-Cu@SiO2@Fe3O4	291:307	In order to effectively remove reactive brilliant red (RBR) in aqueous solution, a novel Cu(II) coordinated chitosan-based magnetic composite (CTS-Cu@SiO2@Fe3O4) was prepared.
32006585	9	15	theme	@	1548:1548	arg1	Fe3O4					1554:1558	CTS-Cu@SiO2@Fe3O4	1542:1558	CTS-Cu@SiO2@Fe3O4	1542:1558	In a word, CTS-Cu@SiO2@Fe3O4 can be served as a promising adsorbent for dye wastewater scavenging.
32006585	8	16	theme	@	1495:1495	arg1	Fe3O4					1501:1505	CTS-Cu@SiO2@Fe3O4	1489:1505	CTS-Cu@SiO2@Fe3O4	1489:1505	Furthermore, regeneration experiments demonstrated that CTS-Cu@SiO2@Fe3O4 can be used repeatedly.
32006585	5	17	theme	adsorption	1133:1142	arg1	data					1144:1147	the equilibrium adsorption data	1117:1147	the equilibrium adsorption data	1117:1147	The adsorption kinetics of CTS-Cu@SiO2@Fe3O4 was found to follow the pseudo-second-order kinetic model, and the equilibrium adsorption data were well described by the Freundlich isotherm model.
32006585	1	18	theme	novel	231:235	arg1	II					240:241	II	240:241	II	240:241	In order to effectively remove reactive brilliant red (RBR) in aqueous solution, a novel Cu(II) coordinated chitosan-based magnetic composite (CTS-Cu@SiO2@Fe3O4) was prepared.
32006585	1	18	theme	novel	231:235	arg1	Cu					237:238	a novel Cu	229:238	a novel Cu(II) coordinated chitosan-based magnetic composite (CTS-Cu@SiO2@Fe3O4)	229:308	In order to effectively remove reactive brilliant red (RBR) in aqueous solution, a novel Cu(II) coordinated chitosan-based magnetic composite (CTS-Cu@SiO2@Fe3O4) was prepared.
32006585	0	19	theme	effective	78:86	arg1	adsorption					88:97	effective adsorption	78:97	effective adsorption of reactive brilliant red from aqueous solution	78:145	Facile fabrication of Cu(II) coordinated chitosan-based magnetic material for effective adsorption of reactive brilliant red from aqueous solution.
32006585	3	20	theme	adsorption	615:624	arg1	capacity					626:633	The adsorption capacity	611:633	The adsorption capacity toward RBR	611:644	The adsorption capacity toward RBR was systematically investigated as a function of contact time, solution pH, initial concentration, ionic strength and temperature.
32006585	3	20	theme	adsorption	615:624	arg1	function					683:690	a function	681:690	a function of contact time, solution pH, initial concentration, ionic strength and temperature	681:774	The adsorption capacity toward RBR was systematically investigated as a function of contact time, solution pH, initial concentration, ionic strength and temperature.
32006585	4	21	theme	@	799:799	arg1	Fe3O4					800:804	CTS@SiO2@Fe3O4	791:804	CTS@SiO2@Fe3O4	791:804	Compared with CTS@SiO2@Fe3O4, CTS-Cu@SiO2@Fe3O4 showed better adsorption performance in removing RBR, reaching a maximum of 880.84 mg/g at pH 4, which confirmed that the coordination of Cu(II) can improve the adsorption capability.
32006585	5	22	theme	Freundlich	1176:1185	arg1	model					1196:1200	the Freundlich isotherm model	1172:1200	the Freundlich isotherm model	1172:1200	The adsorption kinetics of CTS-Cu@SiO2@Fe3O4 was found to follow the pseudo-second-order kinetic model, and the equilibrium adsorption data were well described by the Freundlich isotherm model.
32006585	0	23	from	solution	138:145	arg1	adsorption					88:97	effective adsorption	78:97	effective adsorption of reactive brilliant red from aqueous solution	78:145	Facile fabrication of Cu(II) coordinated chitosan-based magnetic material for effective adsorption of reactive brilliant red from aqueous solution.
32006585	3	24	theme	solution	709:716	arg1	pH					718:719	solution pH	709:719	solution pH	709:719	The adsorption capacity toward RBR was systematically investigated as a function of contact time, solution pH, initial concentration, ionic strength and temperature.
32006585	9	25	theme	@	1553:1553	arg1	Fe3O4					1554:1558	CTS-Cu@SiO2@Fe3O4	1542:1558	CTS-Cu@SiO2@Fe3O4	1542:1558	In a word, CTS-Cu@SiO2@Fe3O4 can be served as a promising adsorbent for dye wastewater scavenging.
32006585	8	26	used	used	1514:1517	arg2	Fe3O4					1501:1505	CTS-Cu@SiO2@Fe3O4	1489:1505	CTS-Cu@SiO2@Fe3O4	1489:1505	Furthermore, regeneration experiments demonstrated that CTS-Cu@SiO2@Fe3O4 can be used repeatedly.
32006585	3	27	theme	ionic	745:749	arg1	strength					751:758	ionic strength	745:758	ionic strength	745:758	The adsorption capacity toward RBR was systematically investigated as a function of contact time, solution pH, initial concentration, ionic strength and temperature.
32006585	3	28	theme	strength	751:758	arg1	function					683:690	a function	681:690	a function of contact time, solution pH, initial concentration, ionic strength and temperature	681:774	The adsorption capacity toward RBR was systematically investigated as a function of contact time, solution pH, initial concentration, ionic strength and temperature.
32006585	3	28	theme	strength	751:758	arg1	capacity					626:633	The adsorption capacity	611:633	The adsorption capacity toward RBR	611:644	The adsorption capacity toward RBR was systematically investigated as a function of contact time, solution pH, initial concentration, ionic strength and temperature.
32006585	8	29	theme	regeneration	1446:1457	arg1	experiments					1459:1469	regeneration experiments	1446:1469	regeneration experiments	1446:1469	Furthermore, regeneration experiments demonstrated that CTS-Cu@SiO2@Fe3O4 can be used repeatedly.
32006585	2	30	theme	electron	555:562	arg1	SEM					576:578	SEM	576:578	SEM	576:578	The physicochemical properties of the resultant adsorbent were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), vibrating sample magnetometer (VSM) thermogravimetric (TG) analysis, scanning electron microscopy (SEM) and N2 adsorption-desorption.
32006585	2	30	theme	electron	555:562	arg1	microscopy					564:573	scanning electron microscopy	546:573	scanning electron microscopy (SEM)	546:579	The physicochemical properties of the resultant adsorbent were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), vibrating sample magnetometer (VSM) thermogravimetric (TG) analysis, scanning electron microscopy (SEM) and N2 adsorption-desorption.
32006585	4	31	theme	better	832:837	arg1	performance					850:860	better adsorption performance	832:860	better adsorption performance	832:860	Compared with CTS@SiO2@Fe3O4, CTS-Cu@SiO2@Fe3O4 showed better adsorption performance in removing RBR, reaching a maximum of 880.84 mg/g at pH 4, which confirmed that the coordination of Cu(II) can improve the adsorption capability.
32006585	2	32	theme	physicochemical	328:342	arg1	properties					344:353	The physicochemical properties	324:353	The physicochemical properties of the resultant adsorbent	324:380	The physicochemical properties of the resultant adsorbent were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), vibrating sample magnetometer (VSM) thermogravimetric (TG) analysis, scanning electron microscopy (SEM) and N2 adsorption-desorption.
32006585	8	33	theme	SiO2	1496:1499	arg1	Fe3O4					1501:1505	CTS-Cu@SiO2@Fe3O4	1489:1505	CTS-Cu@SiO2@Fe3O4	1489:1505	Furthermore, regeneration experiments demonstrated that CTS-Cu@SiO2@Fe3O4 can be used repeatedly.
32006585	2	34	theme	scanning	546:553	arg1	SEM					576:578	SEM	576:578	SEM	576:578	The physicochemical properties of the resultant adsorbent were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), vibrating sample magnetometer (VSM) thermogravimetric (TG) analysis, scanning electron microscopy (SEM) and N2 adsorption-desorption.
32006585	2	34	theme	scanning	546:553	arg1	microscopy					564:573	scanning electron microscopy	546:573	scanning electron microscopy (SEM)	546:579	The physicochemical properties of the resultant adsorbent were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), vibrating sample magnetometer (VSM) thermogravimetric (TG) analysis, scanning electron microscopy (SEM) and N2 adsorption-desorption.
32006585	2	35	theme	X-ray	452:456	arg1	XRD					471:473	XRD	471:473	XRD	471:473	The physicochemical properties of the resultant adsorbent were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), vibrating sample magnetometer (VSM) thermogravimetric (TG) analysis, scanning electron microscopy (SEM) and N2 adsorption-desorption.
32006585	2	35	theme	X-ray	452:456	arg1	diffraction					458:468	X-ray diffraction	452:468	X-ray diffraction (XRD)	452:474	The physicochemical properties of the resultant adsorbent were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), vibrating sample magnetometer (VSM) thermogravimetric (TG) analysis, scanning electron microscopy (SEM) and N2 adsorption-desorption.
32006585	7	36	theme	XPS	1296:1298	arg1	analysis					1300:1307	XPS analysis	1296:1307	XPS analysis	1296:1307	XPS analysis confirmed that the adsorption was mainly controlled by electrostatic interaction between copper/amino cation and RBR anion.
32006585	0	37	theme	Facile	0:5	arg1	fabrication					7:17	Facile fabrication	0:17	Facile fabrication of Cu(II)	0:27	Facile fabrication of Cu(II) coordinated chitosan-based magnetic material for effective adsorption of reactive brilliant red from aqueous solution.
32006585	5	38	theme	SiO2	1043:1046	arg1	Fe3O4					1048:1052	CTS-Cu@SiO2@Fe3O4	1036:1052	CTS-Cu@SiO2@Fe3O4	1036:1052	The adsorption kinetics of CTS-Cu@SiO2@Fe3O4 was found to follow the pseudo-second-order kinetic model, and the equilibrium adsorption data were well described by the Freundlich isotherm model.
32006585	7	39	theme	copper/amino	1398:1409	arg1	cation					1411:1416	copper/amino cation	1398:1416	copper/amino cation	1398:1416	XPS analysis confirmed that the adsorption was mainly controlled by electrostatic interaction between copper/amino cation and RBR anion.
32006585	6	40	theme	Thermodynamic	1203:1215	arg1	studies					1217:1223	Thermodynamic studies	1203:1223	Thermodynamic studies	1203:1223	Thermodynamic studies indicated that the adsorption process was spontaneous and endothermic.
32006585	4	41	theme	@	794:794	arg1	Fe3O4					800:804	CTS@SiO2@Fe3O4	791:804	CTS@SiO2@Fe3O4	791:804	Compared with CTS@SiO2@Fe3O4, CTS-Cu@SiO2@Fe3O4 showed better adsorption performance in removing RBR, reaching a maximum of 880.84 mg/g at pH 4, which confirmed that the coordination of Cu(II) can improve the adsorption capability.
32006585	0	42	theme	Cu	22:23	arg1	fabrication					7:17	Facile fabrication	0:17	Facile fabrication of Cu(II)	0:27	Facile fabrication of Cu(II) coordinated chitosan-based magnetic material for effective adsorption of reactive brilliant red from aqueous solution.
32006585	3	43	theme	contact	695:701	arg1	time					703:706	contact time	695:706	contact time	695:706	The adsorption capacity toward RBR was systematically investigated as a function of contact time, solution pH, initial concentration, ionic strength and temperature.
32006585	0	44	theme	red	121:123	arg1	adsorption					88:97	effective adsorption	78:97	effective adsorption of reactive brilliant red from aqueous solution	78:145	Facile fabrication of Cu(II) coordinated chitosan-based magnetic material for effective adsorption of reactive brilliant red from aqueous solution.
32006585	2	45	theme	N2	585:586	arg1	adsorption-desorption					588:608	N2 adsorption-desorption	585:608	N2 adsorption-desorption	585:608	The physicochemical properties of the resultant adsorbent were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), vibrating sample magnetometer (VSM) thermogravimetric (TG) analysis, scanning electron microscopy (SEM) and N2 adsorption-desorption.
32006585	9	46	theme	CTS-Cu	1542:1547	arg1	Fe3O4					1554:1558	CTS-Cu@SiO2@Fe3O4	1542:1558	CTS-Cu@SiO2@Fe3O4	1542:1558	In a word, CTS-Cu@SiO2@Fe3O4 can be served as a promising adsorbent for dye wastewater scavenging.
32006585	2	47	theme	adsorbent	372:380	arg1	properties					344:353	The physicochemical properties	324:353	The physicochemical properties of the resultant adsorbent	324:380	The physicochemical properties of the resultant adsorbent were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), vibrating sample magnetometer (VSM) thermogravimetric (TG) analysis, scanning electron microscopy (SEM) and N2 adsorption-desorption.
32006585	4	48	theme	CTS	791:793	arg1	Fe3O4					800:804	CTS@SiO2@Fe3O4	791:804	CTS@SiO2@Fe3O4	791:804	Compared with CTS@SiO2@Fe3O4, CTS-Cu@SiO2@Fe3O4 showed better adsorption performance in removing RBR, reaching a maximum of 880.84 mg/g at pH 4, which confirmed that the coordination of Cu(II) can improve the adsorption capability.
32006585	5	49	theme	Fe3O4	1048:1052	arg1	kinetics					1024:1031	The adsorption kinetics	1009:1031	The adsorption kinetics of CTS-Cu@SiO2@Fe3O4	1009:1052	The adsorption kinetics of CTS-Cu@SiO2@Fe3O4 was found to follow the pseudo-second-order kinetic model, and the equilibrium adsorption data were well described by the Freundlich isotherm model.
32006585	4	50	theme	@	818:818	arg1	Fe3O4					819:823	CTS-Cu@SiO2@Fe3O4	807:823	CTS-Cu@SiO2@Fe3O4	807:823	Compared with CTS@SiO2@Fe3O4, CTS-Cu@SiO2@Fe3O4 showed better adsorption performance in removing RBR, reaching a maximum of 880.84 mg/g at pH 4, which confirmed that the coordination of Cu(II) can improve the adsorption capability.
32006585	4	51	theme	adsorption	986:995	arg1	capability					997:1006	the adsorption capability	982:1006	the adsorption capability	982:1006	Compared with CTS@SiO2@Fe3O4, CTS-Cu@SiO2@Fe3O4 showed better adsorption performance in removing RBR, reaching a maximum of 880.84 mg/g at pH 4, which confirmed that the coordination of Cu(II) can improve the adsorption capability.
32006585	0	52	theme	aqueous	130:136	arg1	solution					138:145	aqueous solution	130:145	aqueous solution	130:145	Facile fabrication of Cu(II) coordinated chitosan-based magnetic material for effective adsorption of reactive brilliant red from aqueous solution.
32006585	5	53	theme	isotherm	1187:1194	arg1	model					1196:1200	the Freundlich isotherm model	1172:1200	the Freundlich isotherm model	1172:1200	The adsorption kinetics of CTS-Cu@SiO2@Fe3O4 was found to follow the pseudo-second-order kinetic model, and the equilibrium adsorption data were well described by the Freundlich isotherm model.
32006585	1	54	theme	chitosan-based	256:269	arg1	composite					280:288	chitosan-based magnetic composite	256:288	chitosan-based magnetic composite (CTS-Cu@SiO2@Fe3O4)	256:308	In order to effectively remove reactive brilliant red (RBR) in aqueous solution, a novel Cu(II) coordinated chitosan-based magnetic composite (CTS-Cu@SiO2@Fe3O4) was prepared.
32006585	1	54	theme	chitosan-based	256:269	arg1	Fe3O4					303:307	CTS-Cu@SiO2@Fe3O4	291:307	CTS-Cu@SiO2@Fe3O4	291:307	In order to effectively remove reactive brilliant red (RBR) in aqueous solution, a novel Cu(II) coordinated chitosan-based magnetic composite (CTS-Cu@SiO2@Fe3O4) was prepared.
32006585	7	55	theme	electrostatic	1364:1376	arg1	interaction					1378:1388	electrostatic interaction	1364:1388	electrostatic interaction between copper/amino cation and RBR anion	1364:1430	XPS analysis confirmed that the adsorption was mainly controlled by electrostatic interaction between copper/amino cation and RBR anion.
32006585	8	56	theme	@	1500:1500	arg1	Fe3O4					1501:1505	CTS-Cu@SiO2@Fe3O4	1489:1505	CTS-Cu@SiO2@Fe3O4	1489:1505	Furthermore, regeneration experiments demonstrated that CTS-Cu@SiO2@Fe3O4 can be used repeatedly.
32006585	2	57	theme	infrared	422:429	arg1	spectroscopy					438:449	infrared (FTIR) spectroscopy	422:449	infrared (FTIR) spectroscopy	422:449	The physicochemical properties of the resultant adsorbent were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), vibrating sample magnetometer (VSM) thermogravimetric (TG) analysis, scanning electron microscopy (SEM) and N2 adsorption-desorption.
32006585	1	58	theme	magnetic	271:278	arg1	composite					280:288	chitosan-based magnetic composite	256:288	chitosan-based magnetic composite (CTS-Cu@SiO2@Fe3O4)	256:308	In order to effectively remove reactive brilliant red (RBR) in aqueous solution, a novel Cu(II) coordinated chitosan-based magnetic composite (CTS-Cu@SiO2@Fe3O4) was prepared.
32006585	1	58	theme	magnetic	271:278	arg1	Fe3O4					303:307	CTS-Cu@SiO2@Fe3O4	291:307	CTS-Cu@SiO2@Fe3O4	291:307	In order to effectively remove reactive brilliant red (RBR) in aqueous solution, a novel Cu(II) coordinated chitosan-based magnetic composite (CTS-Cu@SiO2@Fe3O4) was prepared.
32006585	2	59	theme	thermogravimetric	513:529	arg1	analysis					536:543	vibrating sample magnetometer (VSM) thermogravimetric (TG) analysis	477:543	vibrating sample magnetometer (VSM) thermogravimetric (TG) analysis	477:543	The physicochemical properties of the resultant adsorbent were characterized by Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), vibrating sample magnetometer (VSM) thermogravimetric (TG) analysis, scanning electron microscopy (SEM) and N2 adsorption-desorption.
32006585	5	60	theme	@	1047:1047	arg1	Fe3O4					1048:1052	CTS-Cu@SiO2@Fe3O4	1036:1052	CTS-Cu@SiO2@Fe3O4	1036:1052	The adsorption kinetics of CTS-Cu@SiO2@Fe3O4 was found to follow the pseudo-second-order kinetic model, and the equilibrium adsorption data were well described by the Freundlich isotherm model.
32006585	9	61	theme	dye	1603:1605	arg1	scavenging					1618:1627	dye wastewater scavenging	1603:1627	dye wastewater scavenging	1603:1627	In a word, CTS-Cu@SiO2@Fe3O4 can be served as a promising adsorbent for dye wastewater scavenging.
32006585	4	62	theme	880.84 mg/g	901:911	arg1	maximum					890:896	a maximum	888:896	a maximum of 880.84 mg/g	888:911	Compared with CTS@SiO2@Fe3O4, CTS-Cu@SiO2@Fe3O4 showed better adsorption performance in removing RBR, reaching a maximum of 880.84 mg/g at pH 4, which confirmed that the coordination of Cu(II) can improve the adsorption capability.
32006585	3	63	theme	time	703:706	arg1	function					683:690	a function	681:690	a function of contact time, solution pH, initial concentration, ionic strength and temperature	681:774	The adsorption capacity toward RBR was systematically investigated as a function of contact time, solution pH, initial concentration, ionic strength and temperature.
32006585	3	63	theme	time	703:706	arg1	capacity					626:633	The adsorption capacity	611:633	The adsorption capacity toward RBR	611:644	The adsorption capacity toward RBR was systematically investigated as a function of contact time, solution pH, initial concentration, ionic strength and temperature.
32006585	1	64	from	solution	219:226	arg1	red					198:200	red	198:200	red	198:200	In order to effectively remove reactive brilliant red (RBR) in aqueous solution, a novel Cu(II) coordinated chitosan-based magnetic composite (CTS-Cu@SiO2@Fe3O4) was prepared.
32006585	1	64	from	solution	219:226	arg1	RBR					203:205	RBR	203:205	RBR	203:205	In order to effectively remove reactive brilliant red (RBR) in aqueous solution, a novel Cu(II) coordinated chitosan-based magnetic composite (CTS-Cu@SiO2@Fe3O4) was prepared.
32006585	7	65	theme	RBR	1422:1424	arg1	anion					1426:1430	RBR anion	1422:1430	RBR anion	1422:1430	XPS analysis confirmed that the adsorption was mainly controlled by electrostatic interaction between copper/amino cation and RBR anion.
32006585	9	66	theme	wastewater	1607:1616	arg1	scavenging					1618:1627	dye wastewater scavenging	1603:1627	dye wastewater scavenging	1603:1627	In a word, CTS-Cu@SiO2@Fe3O4 can be served as a promising adsorbent for dye wastewater scavenging.
32006585	5	67	theme	@	1042:1042	arg1	Fe3O4					1048:1052	CTS-Cu@SiO2@Fe3O4	1036:1052	CTS-Cu@SiO2@Fe3O4	1036:1052	The adsorption kinetics of CTS-Cu@SiO2@Fe3O4 was found to follow the pseudo-second-order kinetic model, and the equilibrium adsorption data were well described by the Freundlich isotherm model.
32006585	4	68	theme	SiO2	814:817	arg1	Fe3O4					819:823	CTS-Cu@SiO2@Fe3O4	807:823	CTS-Cu@SiO2@Fe3O4	807:823	Compared with CTS@SiO2@Fe3O4, CTS-Cu@SiO2@Fe3O4 showed better adsorption performance in removing RBR, reaching a maximum of 880.84 mg/g at pH 4, which confirmed that the coordination of Cu(II) can improve the adsorption capability.
32006585	1	69	from	red	198:200	arg1	solution					219:226	aqueous solution	211:226	aqueous solution	211:226	In order to effectively remove reactive brilliant red (RBR) in aqueous solution, a novel Cu(II) coordinated chitosan-based magnetic composite (CTS-Cu@SiO2@Fe3O4) was prepared.
32006585	4	70	theme	Cu	963:964	arg1	coordination					947:958	the coordination	943:958	the coordination of Cu(II)	943:968	Compared with CTS@SiO2@Fe3O4, CTS-Cu@SiO2@Fe3O4 showed better adsorption performance in removing RBR, reaching a maximum of 880.84 mg/g at pH 4, which confirmed that the coordination of Cu(II) can improve the adsorption capability.
32006585	3	71	theme	concentration	730:742	arg1	function					683:690	a function	681:690	a function of contact time, solution pH, initial concentration, ionic strength and temperature	681:774	The adsorption capacity toward RBR was systematically investigated as a function of contact time, solution pH, initial concentration, ionic strength and temperature.
32006585	3	71	theme	concentration	730:742	arg1	capacity					626:633	The adsorption capacity	611:633	The adsorption capacity toward RBR	611:644	The adsorption capacity toward RBR was systematically investigated as a function of contact time, solution pH, initial concentration, ionic strength and temperature.
32006585	4	72	theme	@	813:813	arg1	Fe3O4					819:823	CTS-Cu@SiO2@Fe3O4	807:823	CTS-Cu@SiO2@Fe3O4	807:823	Compared with CTS@SiO2@Fe3O4, CTS-Cu@SiO2@Fe3O4 showed better adsorption performance in removing RBR, reaching a maximum of 880.84 mg/g at pH 4, which confirmed that the coordination of Cu(II) can improve the adsorption capability.
32006585	4	73	theme	adsorption	839:848	arg1	performance					850:860	better adsorption performance	832:860	better adsorption performance	832:860	Compared with CTS@SiO2@Fe3O4, CTS-Cu@SiO2@Fe3O4 showed better adsorption performance in removing RBR, reaching a maximum of 880.84 mg/g at pH 4, which confirmed that the coordination of Cu(II) can improve the adsorption capability.
32006585	5	74	theme	kinetic	1098:1104	arg1	model					1106:1110	the pseudo-second-order kinetic model	1074:1110	the pseudo-second-order kinetic model	1074:1110	The adsorption kinetics of CTS-Cu@SiO2@Fe3O4 was found to follow the pseudo-second-order kinetic model, and the equilibrium adsorption data were well described by the Freundlich isotherm model.
32006585	0	75	theme	magnetic	56:63	arg1	material					65:72	chitosan-based magnetic material	41:72	chitosan-based magnetic material for effective adsorption of reactive brilliant red from aqueous solution	41:145	Facile fabrication of Cu(II) coordinated chitosan-based magnetic material for effective adsorption of reactive brilliant red from aqueous solution.
32006585	5	76	theme	adsorption	1013:1022	arg1	kinetics					1024:1031	The adsorption kinetics	1009:1031	The adsorption kinetics of CTS-Cu@SiO2@Fe3O4	1009:1052	The adsorption kinetics of CTS-Cu@SiO2@Fe3O4 was found to follow the pseudo-second-order kinetic model, and the equilibrium adsorption data were well described by the Freundlich isotherm model.
32006585	1	77	theme	aqueous	211:217	arg1	solution					219:226	aqueous solution	211:226	aqueous solution	211:226	In order to effectively remove reactive brilliant red (RBR) in aqueous solution, a novel Cu(II) coordinated chitosan-based magnetic composite (CTS-Cu@SiO2@Fe3O4) was prepared.
32006585	0	78	theme	chitosan-based	41:54	arg1	material					65:72	chitosan-based magnetic material	41:72	chitosan-based magnetic material for effective adsorption of reactive brilliant red from aqueous solution	41:145	Facile fabrication of Cu(II) coordinated chitosan-based magnetic material for effective adsorption of reactive brilliant red from aqueous solution.
32006585	1	79	theme	CTS-Cu	291:296	arg1	composite					280:288	chitosan-based magnetic composite	256:288	chitosan-based magnetic composite (CTS-Cu@SiO2@Fe3O4)	256:308	In order to effectively remove reactive brilliant red (RBR) in aqueous solution, a novel Cu(II) coordinated chitosan-based magnetic composite (CTS-Cu@SiO2@Fe3O4) was prepared.
32006585	1	79	theme	CTS-Cu	291:296	arg1	Fe3O4					303:307	CTS-Cu@SiO2@Fe3O4	291:307	CTS-Cu@SiO2@Fe3O4	291:307	In order to effectively remove reactive brilliant red (RBR) in aqueous solution, a novel Cu(II) coordinated chitosan-based magnetic composite (CTS-Cu@SiO2@Fe3O4) was prepared.
32006585	3	80	theme	initial	722:728	arg1	concentration					730:742	initial concentration	722:742	initial concentration	722:742	The adsorption capacity toward RBR was systematically investigated as a function of contact time, solution pH, initial concentration, ionic strength and temperature.
32006585	5	81	theme	pseudo-second-order	1078:1096	arg1	model					1106:1110	the pseudo-second-order kinetic model	1074:1110	the pseudo-second-order kinetic model	1074:1110	The adsorption kinetics of CTS-Cu@SiO2@Fe3O4 was found to follow the pseudo-second-order kinetic model, and the equilibrium adsorption data were well described by the Freundlich isotherm model.
32006585	3	82	theme	pH	718:719	arg1	function					683:690	a function	681:690	a function of contact time, solution pH, initial concentration, ionic strength and temperature	681:774	The adsorption capacity toward RBR was systematically investigated as a function of contact time, solution pH, initial concentration, ionic strength and temperature.
32006585	3	82	theme	pH	718:719	arg1	capacity					626:633	The adsorption capacity	611:633	The adsorption capacity toward RBR	611:644	The adsorption capacity toward RBR was systematically investigated as a function of contact time, solution pH, initial concentration, ionic strength and temperature.
33759044	0	0	theme	RAW	82:84	arg1	Cells					92:96	RAW 264.7 Cells	82:96	RAW 264.7 Cells	82:96	Protective Effect of Brassica napus L. Hydrosols against Inflammation Response in RAW 264.7 Cells.
33759044	10	1	theme	RAW	1361:1363	arg1	cells					1371:1375	LPS-stimulated RAW 264.7 cells	1346:1375	LPS-stimulated RAW 264.7 cells	1346:1375	CONCLUSION The anti-inflammatory activities of BNH were mediated via blockage of the NF-κB signaling pathways in LPS-stimulated RAW 264.7 cells.
33759044	10	2	theme	pathways	1334:1341	arg1	blockage					1302:1309	blockage	1302:1309	blockage of the NF-κB signaling pathways in LPS-stimulated RAW 264.7 cells	1302:1375	CONCLUSION The anti-inflammatory activities of BNH were mediated via blockage of the NF-κB signaling pathways in LPS-stimulated RAW 264.7 cells.
33759044	1	3	theme	Brassica	158:165	arg1	BNH					187:189	BNH	187:189	BNH	187:189	OBJECTIVE To demonstrate the anti-inflammatory activity of Brassica napus L. hydrosols (BNH) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.
33759044	1	3	theme	Brassica	158:165	arg1	hydrosols					176:184	Brassica napus L. hydrosols	158:184	Brassica napus L. hydrosols (BNH)	158:190	OBJECTIVE To demonstrate the anti-inflammatory activity of Brassica napus L. hydrosols (BNH) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.
33759044	8	4	theme	COX-2	1098:1102	arg1	levels					1079:1084	protein levels	1071:1084	protein levels of iNOS and COX-2 (P<0.01)	1071:1111	Moreover, BNH inhibited protein levels of iNOS and COX-2 (P<0.01).
33759044	4	5	theme	immunosorbent	503:515	arg1	assay					517:521	enzyme-linked immunosorbent assay	489:521	enzyme-linked immunosorbent assay	489:521	Prostaglandin E2 (PGE2) production was evaluated with enzyme-linked immunosorbent assay.
33759044	5	6	theme	nitric	603:608	arg1	iNOS					626:629	iNOS	626:629	iNOS	626:629	The effects of BNH on LPS-induced pro-inflammatory enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were evaluated using Western blot analysis.
33759044	5	6	theme	nitric	603:608	arg1	synthase					616:623	inducible nitric oxide synthase	593:623	inducible nitric oxide synthase (iNOS)	593:630	The effects of BNH on LPS-induced pro-inflammatory enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were evaluated using Western blot analysis.
33759044	7	7	theme	LPS-stimulated	996:1009	arg1	cells					1021:1025	LPS-stimulated RAW 264.7 cells	996:1025	LPS-stimulated RAW 264.7 cells (P<0.01 or P<0.05)	996:1044	RESULTS Compared with LPS-stimulated cells, BNH markedly decreased the generation of NO and PGE2 in LPS-stimulated RAW 264.7 cells (P<0.01 or P<0.05).
33759044	7	7	theme	LPS-stimulated	996:1009	arg1	P<0.05					1038:1043	P<0.05	1038:1043	P<0.05	1038:1043	RESULTS Compared with LPS-stimulated cells, BNH markedly decreased the generation of NO and PGE2 in LPS-stimulated RAW 264.7 cells (P<0.01 or P<0.05).
33759044	7	7	theme	LPS-stimulated	996:1009	arg1	P<0.01					1028:1033	P<0.01	1028:1033	P<0.01	1028:1033	RESULTS Compared with LPS-stimulated cells, BNH markedly decreased the generation of NO and PGE2 in LPS-stimulated RAW 264.7 cells (P<0.01 or P<0.05).
33759044	4	8	theme	enzyme-linked	489:501	arg1	assay					517:521	enzyme-linked immunosorbent assay	489:521	enzyme-linked immunosorbent assay	489:521	Prostaglandin E2 (PGE2) production was evaluated with enzyme-linked immunosorbent assay.
33759044	5	9	theme	oxide	610:614	arg1	iNOS					626:629	iNOS	626:629	iNOS	626:629	The effects of BNH on LPS-induced pro-inflammatory enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were evaluated using Western blot analysis.
33759044	5	9	theme	oxide	610:614	arg1	synthase					616:623	inducible nitric oxide synthase	593:623	inducible nitric oxide synthase (iNOS)	593:630	The effects of BNH on LPS-induced pro-inflammatory enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were evaluated using Western blot analysis.
33759044	2	10	theme	BNH	280:282	arg1	analysis					268:275	Composition analysis	256:275	Composition analysis of BNH	256:282	METHODS Composition analysis of BNH was conducted via gas chromatography-mass spectrometry after BNH were extracted.
33759044	2	11	theme	gas	302:304	arg1	spectrometry					326:337	gas chromatography-mass spectrometry	302:337	gas chromatography-mass spectrometry	302:337	METHODS Composition analysis of BNH was conducted via gas chromatography-mass spectrometry after BNH were extracted.
33759044	10	12	theme	NF-κB	1318:1322	arg1	pathways					1334:1341	the NF-κB signaling pathways	1314:1341	the NF-κB signaling pathways	1314:1341	CONCLUSION The anti-inflammatory activities of BNH were mediated via blockage of the NF-κB signaling pathways in LPS-stimulated RAW 264.7 cells.
33759044	1	13	from	activity	146:153	arg1	cells					241:245	lipopolysaccharide (LPS)-stimulated RAW 264.7 cells	195:245	lipopolysaccharide (LPS)-stimulated RAW 264.7 cells	195:245	OBJECTIVE To demonstrate the anti-inflammatory activity of Brassica napus L. hydrosols (BNH) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.
33759044	10	14	theme	BNH	1280:1282	arg1	activities					1266:1275	The anti-inflammatory activities	1244:1275	The anti-inflammatory activities of BNH	1244:1282	CONCLUSION The anti-inflammatory activities of BNH were mediated via blockage of the NF-κB signaling pathways in LPS-stimulated RAW 264.7 cells.
33759044	1	15	theme	anti-inflammatory	128:144	arg1	activity					146:153	the anti-inflammatory activity	124:153	the anti-inflammatory activity of Brassica napus L. hydrosols (BNH) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells	124:245	OBJECTIVE To demonstrate the anti-inflammatory activity of Brassica napus L. hydrosols (BNH) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.
33759044	6	16	theme	NF-κB	797:801	arg1	p65					803:805	NF-κB p65	797:805	NF-κB p65	797:805	Furthermore, phosphorylation of nuclear factor-kappa B (NF-κB) and nuclear translocation of NF-κB p65 were evaluated with Western blot analysis and immunofluorescence staining, respectively.
33759044	7	17	dep	cells	1021:1025	arg1	cells					1021:1025	LPS-stimulated RAW 264.7 cells	996:1025	LPS-stimulated RAW 264.7 cells (P<0.01 or P<0.05)	996:1044	RESULTS Compared with LPS-stimulated cells, BNH markedly decreased the generation of NO and PGE2 in LPS-stimulated RAW 264.7 cells (P<0.01 or P<0.05).
33759044	7	17	dep	cells	1021:1025	arg1	P<0.05					1038:1043	P<0.05	1038:1043	P<0.05	1038:1043	RESULTS Compared with LPS-stimulated cells, BNH markedly decreased the generation of NO and PGE2 in LPS-stimulated RAW 264.7 cells (P<0.01 or P<0.05).
33759044	7	17	dep	cells	1021:1025	arg1	P<0.01					1028:1033	P<0.01	1028:1033	P<0.01	1028:1033	RESULTS Compared with LPS-stimulated cells, BNH markedly decreased the generation of NO and PGE2 in LPS-stimulated RAW 264.7 cells (P<0.01 or P<0.05).
33759044	3	18	theme	Griess	421:426	arg1	assay					428:432	the Griess assay	417:432	the Griess assay	417:432	The nitric oxide (NO) production was measured using the Griess assay.
33759044	9	19	dep	BNH	1209:1211	arg1	P<0.05					1224:1229	P<0.05	1224:1229	P<0.05	1224:1229	Phosphorylation of NF-κB and nuclear translocation of NF-κB p65 was significantly inhibited by BNH (P<0.01 or P<0.05).
33759044	9	19	dep	BNH	1209:1211	arg1	P<0.01					1214:1219	P<0.01	1214:1219	P<0.01	1214:1219	Phosphorylation of NF-κB and nuclear translocation of NF-κB p65 was significantly inhibited by BNH (P<0.01 or P<0.05).
33759044	5	20	theme	pro-inflammatory	558:573	arg1	cyclooxygenase-2					636:651	cyclooxygenase-2	636:651	cyclooxygenase-2 (COX-2)	636:659	The effects of BNH on LPS-induced pro-inflammatory enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were evaluated using Western blot analysis.
33759044	5	20	theme	pro-inflammatory	558:573	arg1	synthase					616:623	inducible nitric oxide synthase	593:623	inducible nitric oxide synthase (iNOS)	593:630	The effects of BNH on LPS-induced pro-inflammatory enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were evaluated using Western blot analysis.
33759044	5	20	theme	pro-inflammatory	558:573	arg1	enzymes					575:581	LPS-induced pro-inflammatory enzymes	546:581	LPS-induced pro-inflammatory enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2)	546:659	The effects of BNH on LPS-induced pro-inflammatory enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were evaluated using Western blot analysis.
33759044	2	21	theme	Composition	256:266	arg1	analysis					268:275	Composition analysis	256:275	Composition analysis of BNH	256:282	METHODS Composition analysis of BNH was conducted via gas chromatography-mass spectrometry after BNH were extracted.
33759044	10	22	dep	CONCLUSION	1233:1242	arg1	mediated					1289:1296	mediated	1289:1296	were mediated via blockage of the NF-κB signaling pathways in LPS-stimulated RAW 264.7 cells	1284:1375	CONCLUSION The anti-inflammatory activities of BNH were mediated via blockage of the NF-κB signaling pathways in LPS-stimulated RAW 264.7 cells.
33759044	1	23	theme	hydrosols	176:184	arg1	activity					146:153	the anti-inflammatory activity	124:153	the anti-inflammatory activity of Brassica napus L. hydrosols (BNH) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells	124:245	OBJECTIVE To demonstrate the anti-inflammatory activity of Brassica napus L. hydrosols (BNH) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.
33759044	1	24	dep	Brassica	158:165	arg1	L.					173:174	Brassica napus L.	158:174	Brassica napus L. hydrosols (BNH)	158:190	OBJECTIVE To demonstrate the anti-inflammatory activity of Brassica napus L. hydrosols (BNH) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.
33759044	10	25	theme	anti-inflammatory	1248:1264	arg1	activities					1266:1275	The anti-inflammatory activities	1244:1275	The anti-inflammatory activities of BNH	1244:1282	CONCLUSION The anti-inflammatory activities of BNH were mediated via blockage of the NF-κB signaling pathways in LPS-stimulated RAW 264.7 cells.
33759044	2	26	dep	METHODS	248:254	arg1	conducted					288:296	conducted	288:296	was conducted via gas chromatography-mass spectrometry after BNH	284:347	METHODS Composition analysis of BNH was conducted via gas chromatography-mass spectrometry after BNH were extracted.
33759044	0	27	theme	Protective	0:9	arg1	Effect					11:16	Protective Effect	0:16	Protective Effect of Brassica napus L. Hydrosols against Inflammation	0:68	Protective Effect of Brassica napus L. Hydrosols against Inflammation Response in RAW 264.7 Cells.
33759044	6	28	theme	nuclear	772:778	arg1	translocation					780:792	nuclear translocation	772:792	nuclear translocation	772:792	Furthermore, phosphorylation of nuclear factor-kappa B (NF-κB) and nuclear translocation of NF-κB p65 were evaluated with Western blot analysis and immunofluorescence staining, respectively.
33759044	3	29	theme	nitric	369:374	arg1	NO					383:384	NO	383:384	NO	383:384	The nitric oxide (NO) production was measured using the Griess assay.
33759044	3	29	theme	nitric	369:374	arg1	oxide					376:380	nitric oxide	369:380	The nitric oxide (NO) production	365:396	The nitric oxide (NO) production was measured using the Griess assay.
33759044	4	30	link	enzyme-linked	489:501	arg1	assay					517:521	enzyme-linked immunosorbent assay	489:521	enzyme-linked immunosorbent assay	489:521	Prostaglandin E2 (PGE2) production was evaluated with enzyme-linked immunosorbent assay.
33759044	5	31	theme	inducible	593:601	arg1	iNOS					626:629	iNOS	626:629	iNOS	626:629	The effects of BNH on LPS-induced pro-inflammatory enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were evaluated using Western blot analysis.
33759044	5	31	theme	inducible	593:601	arg1	synthase					616:623	inducible nitric oxide synthase	593:623	inducible nitric oxide synthase (iNOS)	593:630	The effects of BNH on LPS-induced pro-inflammatory enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were evaluated using Western blot analysis.
33759044	9	32	theme	NF-κB	1168:1172	arg1	p65					1174:1176	NF-κB p65	1168:1176	NF-κB p65	1168:1176	Phosphorylation of NF-κB and nuclear translocation of NF-κB p65 was significantly inhibited by BNH (P<0.01 or P<0.05).
33759044	7	33	theme	NO	981:982	arg1	generation					967:976	the generation	963:976	the generation of NO and PGE2	963:991	RESULTS Compared with LPS-stimulated cells, BNH markedly decreased the generation of NO and PGE2 in LPS-stimulated RAW 264.7 cells (P<0.01 or P<0.05).
33759044	3	34	theme	oxide	376:380	arg1	production					387:396	The nitric oxide (NO) production	365:396	The nitric oxide (NO) production	365:396	The nitric oxide (NO) production was measured using the Griess assay.
33759044	8	35	theme	protein	1071:1077	arg1	levels					1079:1084	protein levels	1071:1084	protein levels of iNOS and COX-2 (P<0.01)	1071:1111	Moreover, BNH inhibited protein levels of iNOS and COX-2 (P<0.01).
33759044	4	36	theme	Prostaglandin	435:447	arg1	PGE2					453:456	PGE2	453:456	PGE2	453:456	Prostaglandin E2 (PGE2) production was evaluated with enzyme-linked immunosorbent assay.
33759044	4	36	theme	Prostaglandin	435:447	arg1	E2					449:450	Prostaglandin E2	435:450	Prostaglandin E2 (PGE2) production	435:468	Prostaglandin E2 (PGE2) production was evaluated with enzyme-linked immunosorbent assay.
33759044	9	37	theme	p65	1174:1176	arg1	Phosphorylation					1114:1128	Phosphorylation	1114:1128	Phosphorylation of NF-κB	1114:1137	Phosphorylation of NF-κB and nuclear translocation of NF-κB p65 was significantly inhibited by BNH (P<0.01 or P<0.05).
33759044	9	37	theme	p65	1174:1176	arg1	translocation					1151:1163	nuclear translocation	1143:1163	nuclear translocation of NF-κB p65	1143:1176	Phosphorylation of NF-κB and nuclear translocation of NF-κB p65 was significantly inhibited by BNH (P<0.01 or P<0.05).
33759044	8	38	theme	iNOS	1089:1092	arg1	levels					1079:1084	protein levels	1071:1084	protein levels of iNOS and COX-2 (P<0.01)	1071:1111	Moreover, BNH inhibited protein levels of iNOS and COX-2 (P<0.01).
33759044	7	39	theme	LPS-stimulated	918:931	arg1	cells					933:937	LPS-stimulated cells	918:937	LPS-stimulated cells	918:937	RESULTS Compared with LPS-stimulated cells, BNH markedly decreased the generation of NO and PGE2 in LPS-stimulated RAW 264.7 cells (P<0.01 or P<0.05).
33759044	6	40	theme	B	758:758	arg1	translocation					780:792	nuclear translocation	772:792	nuclear translocation	772:792	Furthermore, phosphorylation of nuclear factor-kappa B (NF-κB) and nuclear translocation of NF-κB p65 were evaluated with Western blot analysis and immunofluorescence staining, respectively.
33759044	6	40	theme	B	758:758	arg1	phosphorylation					718:732	phosphorylation	718:732	phosphorylation of nuclear factor-kappa B (NF-κB)	718:766	Furthermore, phosphorylation of nuclear factor-kappa B (NF-κB) and nuclear translocation of NF-κB p65 were evaluated with Western blot analysis and immunofluorescence staining, respectively.
33759044	0	41	from	Response	70:77	arg1	Cells					92:96	RAW 264.7 Cells	82:96	RAW 264.7 Cells	82:96	Protective Effect of Brassica napus L. Hydrosols against Inflammation Response in RAW 264.7 Cells.
33759044	10	42	theme	signaling	1324:1332	arg1	pathways					1334:1341	the NF-κB signaling pathways	1314:1341	the NF-κB signaling pathways	1314:1341	CONCLUSION The anti-inflammatory activities of BNH were mediated via blockage of the NF-κB signaling pathways in LPS-stimulated RAW 264.7 cells.
33759044	6	43	theme	p65	803:805	arg1	translocation					780:792	nuclear translocation	772:792	nuclear translocation	772:792	Furthermore, phosphorylation of nuclear factor-kappa B (NF-κB) and nuclear translocation of NF-κB p65 were evaluated with Western blot analysis and immunofluorescence staining, respectively.
33759044	6	43	theme	p65	803:805	arg1	phosphorylation					718:732	phosphorylation	718:732	phosphorylation of nuclear factor-kappa B (NF-κB)	718:766	Furthermore, phosphorylation of nuclear factor-kappa B (NF-κB) and nuclear translocation of NF-κB p65 were evaluated with Western blot analysis and immunofluorescence staining, respectively.
33759044	4	44	theme	E2	449:450	arg1	production					459:468	Prostaglandin E2 (PGE2) production	435:468	Prostaglandin E2 (PGE2) production	435:468	Prostaglandin E2 (PGE2) production was evaluated with enzyme-linked immunosorbent assay.
33759044	5	45	theme	BNH	539:541	arg1	effects					528:534	The effects	524:534	The effects of BNH on LPS-induced pro-inflammatory enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2)	524:659	The effects of BNH on LPS-induced pro-inflammatory enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were evaluated using Western blot analysis.
33759044	0	46	theme	Hydrosols	39:47	arg1	Effect					11:16	Protective Effect	0:16	Protective Effect of Brassica napus L. Hydrosols against Inflammation	0:68	Protective Effect of Brassica napus L. Hydrosols against Inflammation Response in RAW 264.7 Cells.
33759044	9	47	theme	NF-κB	1133:1137	arg1	Phosphorylation					1114:1128	Phosphorylation	1114:1128	Phosphorylation of NF-κB	1114:1137	Phosphorylation of NF-κB and nuclear translocation of NF-κB p65 was significantly inhibited by BNH (P<0.01 or P<0.05).
33759044	9	47	theme	NF-κB	1133:1137	arg1	translocation					1151:1163	nuclear translocation	1143:1163	nuclear translocation of NF-κB p65	1143:1176	Phosphorylation of NF-κB and nuclear translocation of NF-κB p65 was significantly inhibited by BNH (P<0.01 or P<0.05).
33759044	6	48	theme	immunofluorescence	853:870	arg1	staining					872:879	immunofluorescence staining	853:879	immunofluorescence staining	853:879	Furthermore, phosphorylation of nuclear factor-kappa B (NF-κB) and nuclear translocation of NF-κB p65 were evaluated with Western blot analysis and immunofluorescence staining, respectively.
33759044	7	49	theme	RAW	1011:1013	arg1	cells					1021:1025	LPS-stimulated RAW 264.7 cells	996:1025	LPS-stimulated RAW 264.7 cells (P<0.01 or P<0.05)	996:1044	RESULTS Compared with LPS-stimulated cells, BNH markedly decreased the generation of NO and PGE2 in LPS-stimulated RAW 264.7 cells (P<0.01 or P<0.05).
33759044	7	49	theme	RAW	1011:1013	arg1	P<0.05					1038:1043	P<0.05	1038:1043	P<0.05	1038:1043	RESULTS Compared with LPS-stimulated cells, BNH markedly decreased the generation of NO and PGE2 in LPS-stimulated RAW 264.7 cells (P<0.01 or P<0.05).
33759044	7	49	theme	RAW	1011:1013	arg1	P<0.01					1028:1033	P<0.01	1028:1033	P<0.01	1028:1033	RESULTS Compared with LPS-stimulated cells, BNH markedly decreased the generation of NO and PGE2 in LPS-stimulated RAW 264.7 cells (P<0.01 or P<0.05).
33759044	5	50	theme	Western	682:688	arg1	analysis					695:702	Western blot analysis	682:702	Western blot analysis	682:702	The effects of BNH on LPS-induced pro-inflammatory enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were evaluated using Western blot analysis.
33759044	6	51	theme	blot	835:838	arg1	analysis					840:847	Western blot analysis	827:847	Western blot analysis	827:847	Furthermore, phosphorylation of nuclear factor-kappa B (NF-κB) and nuclear translocation of NF-κB p65 were evaluated with Western blot analysis and immunofluorescence staining, respectively.
33759044	5	52	theme	LPS-induced	546:556	arg1	cyclooxygenase-2					636:651	cyclooxygenase-2	636:651	cyclooxygenase-2 (COX-2)	636:659	The effects of BNH on LPS-induced pro-inflammatory enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were evaluated using Western blot analysis.
33759044	5	52	theme	LPS-induced	546:556	arg1	synthase					616:623	inducible nitric oxide synthase	593:623	inducible nitric oxide synthase (iNOS)	593:630	The effects of BNH on LPS-induced pro-inflammatory enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were evaluated using Western blot analysis.
33759044	5	52	theme	LPS-induced	546:556	arg1	enzymes					575:581	LPS-induced pro-inflammatory enzymes	546:581	LPS-induced pro-inflammatory enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2)	546:659	The effects of BNH on LPS-induced pro-inflammatory enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were evaluated using Western blot analysis.
33759044	9	53	theme	nuclear	1143:1149	arg1	translocation					1151:1163	nuclear translocation	1143:1163	nuclear translocation of NF-κB p65	1143:1176	Phosphorylation of NF-κB and nuclear translocation of NF-κB p65 was significantly inhibited by BNH (P<0.01 or P<0.05).
33759044	5	54	theme	blot	690:693	arg1	analysis					695:702	Western blot analysis	682:702	Western blot analysis	682:702	The effects of BNH on LPS-induced pro-inflammatory enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were evaluated using Western blot analysis.
33759044	6	55	theme	Western	827:833	arg1	analysis					840:847	Western blot analysis	827:847	Western blot analysis	827:847	Furthermore, phosphorylation of nuclear factor-kappa B (NF-κB) and nuclear translocation of NF-κB p65 were evaluated with Western blot analysis and immunofluorescence staining, respectively.
33759044	6	56	theme	factor-kappa	745:756	arg1	NF-κB					761:765	NF-κB	761:765	NF-κB	761:765	Furthermore, phosphorylation of nuclear factor-kappa B (NF-κB) and nuclear translocation of NF-κB p65 were evaluated with Western blot analysis and immunofluorescence staining, respectively.
33759044	6	56	theme	factor-kappa	745:756	arg1	B					758:758	nuclear factor-kappa B	737:758	nuclear factor-kappa B (NF-κB)	737:766	Furthermore, phosphorylation of nuclear factor-kappa B (NF-κB) and nuclear translocation of NF-κB p65 were evaluated with Western blot analysis and immunofluorescence staining, respectively.
33759044	2	57	theme	chromatography-mass	306:324	arg1	spectrometry					326:337	gas chromatography-mass spectrometry	302:337	gas chromatography-mass spectrometry	302:337	METHODS Composition analysis of BNH was conducted via gas chromatography-mass spectrometry after BNH were extracted.
33759044	10	58	theme	LPS-stimulated	1346:1359	arg1	cells					1371:1375	LPS-stimulated RAW 264.7 cells	1346:1375	LPS-stimulated RAW 264.7 cells	1346:1375	CONCLUSION The anti-inflammatory activities of BNH were mediated via blockage of the NF-κB signaling pathways in LPS-stimulated RAW 264.7 cells.
33759044	10	59	from	blockage	1302:1309	arg1	cells					1371:1375	LPS-stimulated RAW 264.7 cells	1346:1375	LPS-stimulated RAW 264.7 cells	1346:1375	CONCLUSION The anti-inflammatory activities of BNH were mediated via blockage of the NF-κB signaling pathways in LPS-stimulated RAW 264.7 cells.
33759044	6	60	theme	nuclear	737:743	arg1	NF-κB					761:765	NF-κB	761:765	NF-κB	761:765	Furthermore, phosphorylation of nuclear factor-kappa B (NF-κB) and nuclear translocation of NF-κB p65 were evaluated with Western blot analysis and immunofluorescence staining, respectively.
33759044	6	60	theme	nuclear	737:743	arg1	B					758:758	nuclear factor-kappa B	737:758	nuclear factor-kappa B (NF-κB)	737:766	Furthermore, phosphorylation of nuclear factor-kappa B (NF-κB) and nuclear translocation of NF-κB p65 were evaluated with Western blot analysis and immunofluorescence staining, respectively.
33759044	5	61	from	effects	528:534	arg1	cyclooxygenase-2					636:651	cyclooxygenase-2	636:651	cyclooxygenase-2 (COX-2)	636:659	The effects of BNH on LPS-induced pro-inflammatory enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were evaluated using Western blot analysis.
33759044	5	61	from	effects	528:534	arg1	synthase					616:623	inducible nitric oxide synthase	593:623	inducible nitric oxide synthase (iNOS)	593:630	The effects of BNH on LPS-induced pro-inflammatory enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were evaluated using Western blot analysis.
33759044	5	61	from	effects	528:534	arg1	enzymes					575:581	LPS-induced pro-inflammatory enzymes	546:581	LPS-induced pro-inflammatory enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2)	546:659	The effects of BNH on LPS-induced pro-inflammatory enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were evaluated using Western blot analysis.
33759044	1	62	theme	-stimulated	219:229	arg1	cells					241:245	lipopolysaccharide (LPS)-stimulated RAW 264.7 cells	195:245	lipopolysaccharide (LPS)-stimulated RAW 264.7 cells	195:245	OBJECTIVE To demonstrate the anti-inflammatory activity of Brassica napus L. hydrosols (BNH) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.
33759044	7	63	theme	PGE2	988:991	arg1	generation					967:976	the generation	963:976	the generation of NO and PGE2	963:991	RESULTS Compared with LPS-stimulated cells, BNH markedly decreased the generation of NO and PGE2 in LPS-stimulated RAW 264.7 cells (P<0.01 or P<0.05).
33759044	1	64	theme	RAW	231:233	arg1	cells					241:245	lipopolysaccharide (LPS)-stimulated RAW 264.7 cells	195:245	lipopolysaccharide (LPS)-stimulated RAW 264.7 cells	195:245	OBJECTIVE To demonstrate the anti-inflammatory activity of Brassica napus L. hydrosols (BNH) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.
33222240	5	0	theme	pathways	1202:1209	arg1	metabolism					1092:1101	the metabolism	1088:1101	the metabolism of taurine and hydroxytaurine, pantothenate and coenzyme A (CoA) biosynthesis, and other signaling pathways	1088:1209	RESULTS We observed that stachyose significantly altered the composition of the intestinal microbiota and metabolites, up-regulated production of the metabolite taurocholic acid, down-regulated amino acid metabolism, and significantly regulated the metabolism of taurine and hydroxytaurine, pantothenate and coenzyme A (CoA) biosynthesis, and other signaling pathways.
33222240	4	1	theme	stachyose	735:743	arg1	intake					745:750	stachyose intake	735:750	stachyose intake	735:750	Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was used to study the effects of stachyose intake on the metabolites and metabolic pathways of the transplanted human intestinal microbiota.
33222240	3	2	theme	intake	509:514	arg1	effect					479:484	the effect	475:484	the effect of long-term stachyose intake on intestinal metabolism	475:539	Therefore, in this study, the feces of infants were transplanted into germ-free mice, and the effect of long-term stachyose intake on intestinal metabolism was examined by comparing the results of microbiome and metabolome analyses.
33222240	4	3	from	effects	724:730	arg1	pathways					785:792	metabolic pathways	775:792	metabolic pathways	775:792	Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was used to study the effects of stachyose intake on the metabolites and metabolic pathways of the transplanted human intestinal microbiota.
33222240	4	3	from	effects	724:730	arg1	metabolites					759:769	metabolites	759:769	metabolites	759:769	Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was used to study the effects of stachyose intake on the metabolites and metabolic pathways of the transplanted human intestinal microbiota.
33222240	5	4	theme	acid	1016:1019	arg1	production					975:984	production	975:984	production of the metabolite taurocholic acid	975:1019	RESULTS We observed that stachyose significantly altered the composition of the intestinal microbiota and metabolites, up-regulated production of the metabolite taurocholic acid, down-regulated amino acid metabolism, and significantly regulated the metabolism of taurine and hydroxytaurine, pantothenate and coenzyme A (CoA) biosynthesis, and other signaling pathways.
33222240	4	5	theme	transplanted	801:812	arg1	microbiota					831:840	the transplanted human intestinal microbiota	797:840	the transplanted human intestinal microbiota	797:840	Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was used to study the effects of stachyose intake on the metabolites and metabolic pathways of the transplanted human intestinal microbiota.
33222240	4	6	dep	metabolites	759:769	arg1	the					755:757	the	755:757	the	755:757	Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was used to study the effects of stachyose intake on the metabolites and metabolic pathways of the transplanted human intestinal microbiota.
33222240	5	7	theme	biosynthesis	1168:1179	arg1	metabolism					1092:1101	the metabolism	1088:1101	the metabolism of taurine and hydroxytaurine, pantothenate and coenzyme A (CoA) biosynthesis, and other signaling pathways	1088:1209	RESULTS We observed that stachyose significantly altered the composition of the intestinal microbiota and metabolites, up-regulated production of the metabolite taurocholic acid, down-regulated amino acid metabolism, and significantly regulated the metabolism of taurine and hydroxytaurine, pantothenate and coenzyme A (CoA) biosynthesis, and other signaling pathways.
33222240	1	8	dep	BACKGROUND	154:163	arg1	microbiota					180:189	The intestinal microbiota	165:189	The intestinal microbiota	165:189	BACKGROUND The intestinal microbiota and metabolites play an important role in human health and immunity.
33222240	2	9	theme	long-term	303:311	arg1	effects					313:319	the long-term effects	299:319	the long-term effects of stachyose on the human intestinal microbiota and metabolism	299:382	However, few studies have investigated the long-term effects of stachyose on the human intestinal microbiota and metabolism.
33222240	5	10	theme	taurocholic	1004:1014	arg1	acid					1016:1019	the metabolite taurocholic acid	989:1019	the metabolite taurocholic acid	989:1019	RESULTS We observed that stachyose significantly altered the composition of the intestinal microbiota and metabolites, up-regulated production of the metabolite taurocholic acid, down-regulated amino acid metabolism, and significantly regulated the metabolism of taurine and hydroxytaurine, pantothenate and coenzyme A (CoA) biosynthesis, and other signaling pathways.
33222240	0	11	theme	intestinal	115:124	arg1	mice					148:151	intestinal microbiota-associated mice	115:151	intestinal microbiota-associated mice	115:151	Microbiome-metabolomic analyses of the impacts of dietary stachyose on fecal microbiota and metabolites in infants intestinal microbiota-associated mice.
33222240	5	12	dep	RESULTS	843:849	arg1	observed					854:861	observed	854:861	observed that stachyose significantly altered the composition of the intestinal microbiota and metabolites, up-regulated production of the metabolite taurocholic acid, down-regulated amino acid metabolism, and significantly regulated the metabolism of taurine and hydroxytaurine, pantothenate and coenzyme A (CoA) biosynthesis, and other signaling pathways.	854:1210	RESULTS We observed that stachyose significantly altered the composition of the intestinal microbiota and metabolites, up-regulated production of the metabolite taurocholic acid, down-regulated amino acid metabolism, and significantly regulated the metabolism of taurine and hydroxytaurine, pantothenate and coenzyme A (CoA) biosynthesis, and other signaling pathways.
33222240	4	13	theme	intestinal	820:829	arg1	microbiota					831:840	the transplanted human intestinal microbiota	797:840	the transplanted human intestinal microbiota	797:840	Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was used to study the effects of stachyose intake on the metabolites and metabolic pathways of the transplanted human intestinal microbiota.
33222240	5	14	theme	metabolite	993:1002	arg1	acid					1016:1019	the metabolite taurocholic acid	989:1019	the metabolite taurocholic acid	989:1019	RESULTS We observed that stachyose significantly altered the composition of the intestinal microbiota and metabolites, up-regulated production of the metabolite taurocholic acid, down-regulated amino acid metabolism, and significantly regulated the metabolism of taurine and hydroxytaurine, pantothenate and coenzyme A (CoA) biosynthesis, and other signaling pathways.
33222240	3	15	from	effect	479:484	arg1	metabolism					530:539	intestinal metabolism	519:539	intestinal metabolism	519:539	Therefore, in this study, the feces of infants were transplanted into germ-free mice, and the effect of long-term stachyose intake on intestinal metabolism was examined by comparing the results of microbiome and metabolome analyses.
33222240	4	16	theme	human	814:818	arg1	microbiota					831:840	the transplanted human intestinal microbiota	797:840	the transplanted human intestinal microbiota	797:840	Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was used to study the effects of stachyose intake on the metabolites and metabolic pathways of the transplanted human intestinal microbiota.
33222240	3	17	theme	germ-free	455:463	arg1	mice					465:468	germ-free mice	455:468	germ-free mice	455:468	Therefore, in this study, the feces of infants were transplanted into germ-free mice, and the effect of long-term stachyose intake on intestinal metabolism was examined by comparing the results of microbiome and metabolome analyses.
33222240	5	18	theme	other	1186:1190	arg1	pathways					1202:1209	other signaling pathways	1186:1209	other signaling pathways	1186:1209	RESULTS We observed that stachyose significantly altered the composition of the intestinal microbiota and metabolites, up-regulated production of the metabolite taurocholic acid, down-regulated amino acid metabolism, and significantly regulated the metabolism of taurine and hydroxytaurine, pantothenate and coenzyme A (CoA) biosynthesis, and other signaling pathways.
33222240	4	19	theme	metabolic	775:783	arg1	pathways					785:792	metabolic pathways	775:792	metabolic pathways	775:792	Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was used to study the effects of stachyose intake on the metabolites and metabolic pathways of the transplanted human intestinal microbiota.
33222240	7	20	theme	Chemical	1347:1354	arg1	Industry					1356:1363	Chemical Industry	1347:1363	Chemical Industry	1347:1363	© 2020 Society of Chemical Industry.
33222240	5	21	theme	microbiota	934:943	arg1	composition					904:914	the composition	900:914	the composition of the intestinal microbiota and metabolites	900:959	RESULTS We observed that stachyose significantly altered the composition of the intestinal microbiota and metabolites, up-regulated production of the metabolite taurocholic acid, down-regulated amino acid metabolism, and significantly regulated the metabolism of taurine and hydroxytaurine, pantothenate and coenzyme A (CoA) biosynthesis, and other signaling pathways.
33222240	0	22	theme	Microbiome-metabolomic	0:21	arg1	analyses					23:30	Microbiome-metabolomic analyses	0:30	Microbiome-metabolomic analyses of the impacts of dietary	0:56	Microbiome-metabolomic analyses of the impacts of dietary stachyose on fecal microbiota and metabolites in infants intestinal microbiota-associated mice.
33222240	5	23	theme	coenzyme	1151:1158	arg1	biosynthesis					1168:1179	pantothenate and coenzyme A (CoA) biosynthesis	1134:1179	biosynthesis	1168:1179	RESULTS We observed that stachyose significantly altered the composition of the intestinal microbiota and metabolites, up-regulated production of the metabolite taurocholic acid, down-regulated amino acid metabolism, and significantly regulated the metabolism of taurine and hydroxytaurine, pantothenate and coenzyme A (CoA) biosynthesis, and other signaling pathways.
33222240	0	24	dep	microbiota	77:86	arg1	mice					148:151	intestinal microbiota-associated mice	115:151	intestinal microbiota-associated mice	115:151	Microbiome-metabolomic analyses of the impacts of dietary stachyose on fecal microbiota and metabolites in infants intestinal microbiota-associated mice.
33222240	1	25	theme	important	215:223	arg1	role					225:228	an important role	212:228	an important role	212:228	BACKGROUND The intestinal microbiota and metabolites play an important role in human health and immunity.
33222240	6	26	dep	CONCLUSION	1212:1221	arg1	provide					1242:1248	provide	1242:1248	may provide a basis for elucidating the mechanism by which stachyose promotes host health	1238:1326	CONCLUSION These findings may provide a basis for elucidating the mechanism by which stachyose promotes host health.
33222240	5	27	theme	hydroxytaurine	1118:1131	arg1	metabolism					1092:1101	the metabolism	1088:1101	the metabolism of taurine and hydroxytaurine, pantothenate and coenzyme A (CoA) biosynthesis, and other signaling pathways	1088:1209	RESULTS We observed that stachyose significantly altered the composition of the intestinal microbiota and metabolites, up-regulated production of the metabolite taurocholic acid, down-regulated amino acid metabolism, and significantly regulated the metabolism of taurine and hydroxytaurine, pantothenate and coenzyme A (CoA) biosynthesis, and other signaling pathways.
33222240	2	28	theme	stachyose	324:332	arg1	effects					313:319	the long-term effects	299:319	the long-term effects of stachyose on the human intestinal microbiota and metabolism	299:382	However, few studies have investigated the long-term effects of stachyose on the human intestinal microbiota and metabolism.
33222240	5	29	theme	acid	1043:1046	arg1	metabolism					1048:1057	amino acid metabolism	1037:1057	amino acid metabolism	1037:1057	RESULTS We observed that stachyose significantly altered the composition of the intestinal microbiota and metabolites, up-regulated production of the metabolite taurocholic acid, down-regulated amino acid metabolism, and significantly regulated the metabolism of taurine and hydroxytaurine, pantothenate and coenzyme A (CoA) biosynthesis, and other signaling pathways.
33222240	3	30	theme	microbiome	582:591	arg1	analyses					608:615	microbiome and metabolome analyses	582:615	microbiome and metabolome analyses	582:615	Therefore, in this study, the feces of infants were transplanted into germ-free mice, and the effect of long-term stachyose intake on intestinal metabolism was examined by comparing the results of microbiome and metabolome analyses.
33222240	0	31	theme	microbiota-associated	126:146	arg1	mice					148:151	intestinal microbiota-associated mice	115:151	intestinal microbiota-associated mice	115:151	Microbiome-metabolomic analyses of the impacts of dietary stachyose on fecal microbiota and metabolites in infants intestinal microbiota-associated mice.
33222240	5	32	theme	A	1160:1160	arg1	biosynthesis					1168:1179	pantothenate and coenzyme A (CoA) biosynthesis	1134:1179	biosynthesis	1168:1179	RESULTS We observed that stachyose significantly altered the composition of the intestinal microbiota and metabolites, up-regulated production of the metabolite taurocholic acid, down-regulated amino acid metabolism, and significantly regulated the metabolism of taurine and hydroxytaurine, pantothenate and coenzyme A (CoA) biosynthesis, and other signaling pathways.
33222240	5	33	theme	amino	1037:1041	arg1	metabolism					1048:1057	amino acid metabolism	1037:1057	amino acid metabolism	1037:1057	RESULTS We observed that stachyose significantly altered the composition of the intestinal microbiota and metabolites, up-regulated production of the metabolite taurocholic acid, down-regulated amino acid metabolism, and significantly regulated the metabolism of taurine and hydroxytaurine, pantothenate and coenzyme A (CoA) biosynthesis, and other signaling pathways.
33222240	5	34	theme	metabolites	949:959	arg1	composition					904:914	the composition	900:914	the composition of the intestinal microbiota and metabolites	900:959	RESULTS We observed that stachyose significantly altered the composition of the intestinal microbiota and metabolites, up-regulated production of the metabolite taurocholic acid, down-regulated amino acid metabolism, and significantly regulated the metabolism of taurine and hydroxytaurine, pantothenate and coenzyme A (CoA) biosynthesis, and other signaling pathways.
33222240	4	35	theme	intake	745:750	arg1	effects					724:730	the effects	720:730	the effects of stachyose intake on the metabolites and metabolic pathways of the transplanted human intestinal microbiota	720:840	Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was used to study the effects of stachyose intake on the metabolites and metabolic pathways of the transplanted human intestinal microbiota.
33222240	0	36	from	microbiota	77:86	arg1	infants					107:113	infants	107:113	infants	107:113	Microbiome-metabolomic analyses of the impacts of dietary stachyose on fecal microbiota and metabolites in infants intestinal microbiota-associated mice.
33222240	3	37	theme	analyses	608:615	arg1	results					571:577	the results	567:577	the results of microbiome and metabolome analyses	567:615	Therefore, in this study, the feces of infants were transplanted into germ-free mice, and the effect of long-term stachyose intake on intestinal metabolism was examined by comparing the results of microbiome and metabolome analyses.
33222240	1	38	theme	human	233:237	arg1	health					239:244	human health	233:244	human health	233:244	BACKGROUND The intestinal microbiota and metabolites play an important role in human health and immunity.
33222240	0	39	theme	impacts	39:45	arg1	analyses					23:30	Microbiome-metabolomic analyses	0:30	Microbiome-metabolomic analyses of the impacts of dietary	0:56	Microbiome-metabolomic analyses of the impacts of dietary stachyose on fecal microbiota and metabolites in infants intestinal microbiota-associated mice.
33222240	3	40	theme	infants	424:430	arg1	feces					415:419	the feces	411:419	the feces of infants	411:430	Therefore, in this study, the feces of infants were transplanted into germ-free mice, and the effect of long-term stachyose intake on intestinal metabolism was examined by comparing the results of microbiome and metabolome analyses.
33222240	5	41	theme	CoA	1163:1165	arg1	biosynthesis					1168:1179	pantothenate and coenzyme A (CoA) biosynthesis	1134:1179	biosynthesis	1168:1179	RESULTS We observed that stachyose significantly altered the composition of the intestinal microbiota and metabolites, up-regulated production of the metabolite taurocholic acid, down-regulated amino acid metabolism, and significantly regulated the metabolism of taurine and hydroxytaurine, pantothenate and coenzyme A (CoA) biosynthesis, and other signaling pathways.
33222240	5	42	theme	taurine	1106:1112	arg1	metabolism					1092:1101	the metabolism	1088:1101	the metabolism of taurine and hydroxytaurine, pantothenate and coenzyme A (CoA) biosynthesis, and other signaling pathways	1088:1209	RESULTS We observed that stachyose significantly altered the composition of the intestinal microbiota and metabolites, up-regulated production of the metabolite taurocholic acid, down-regulated amino acid metabolism, and significantly regulated the metabolism of taurine and hydroxytaurine, pantothenate and coenzyme A (CoA) biosynthesis, and other signaling pathways.
33222240	3	43	theme	long-term	489:497	arg1	intake					509:514	long-term stachyose intake	489:514	long-term stachyose intake	489:514	Therefore, in this study, the feces of infants were transplanted into germ-free mice, and the effect of long-term stachyose intake on intestinal metabolism was examined by comparing the results of microbiome and metabolome analyses.
33222240	0	44	theme	dietary	50:56	arg1	impacts					39:45	the impacts	35:45	the impacts of dietary	35:56	Microbiome-metabolomic analyses of the impacts of dietary stachyose on fecal microbiota and metabolites in infants intestinal microbiota-associated mice.
33222240	6	45	theme	host	1316:1319	arg1	health					1321:1326	host health	1316:1326	host health	1316:1326	CONCLUSION These findings may provide a basis for elucidating the mechanism by which stachyose promotes host health.
33222240	3	46	theme	metabolome	597:606	arg1	analyses					608:615	microbiome and metabolome analyses	582:615	microbiome and metabolome analyses	582:615	Therefore, in this study, the feces of infants were transplanted into germ-free mice, and the effect of long-term stachyose intake on intestinal metabolism was examined by comparing the results of microbiome and metabolome analyses.
33222240	3	47	theme	stachyose	499:507	arg1	intake					509:514	long-term stachyose intake	489:514	long-term stachyose intake	489:514	Therefore, in this study, the feces of infants were transplanted into germ-free mice, and the effect of long-term stachyose intake on intestinal metabolism was examined by comparing the results of microbiome and metabolome analyses.
33222240	5	48	theme	pantothenate	1134:1145	arg1	biosynthesis					1168:1179	pantothenate and coenzyme A (CoA) biosynthesis	1134:1179	biosynthesis	1168:1179	RESULTS We observed that stachyose significantly altered the composition of the intestinal microbiota and metabolites, up-regulated production of the metabolite taurocholic acid, down-regulated amino acid metabolism, and significantly regulated the metabolism of taurine and hydroxytaurine, pantothenate and coenzyme A (CoA) biosynthesis, and other signaling pathways.
33222240	4	49	used	used	706:709	arg2	spectrometry					675:686	Ultra-high-performance liquid chromatography-tandem mass spectrometry	618:686	Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)	618:700	Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was used to study the effects of stachyose intake on the metabolites and metabolic pathways of the transplanted human intestinal microbiota.
33222240	4	49	used	used	706:709	arg2	UHPLC-MS/MS					689:699	UHPLC-MS/MS	689:699	UHPLC-MS/MS	689:699	Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was used to study the effects of stachyose intake on the metabolites and metabolic pathways of the transplanted human intestinal microbiota.
33222240	2	50	theme	intestinal	347:356	arg1	microbiota					358:367	the human intestinal microbiota	337:367	the human intestinal microbiota	337:367	However, few studies have investigated the long-term effects of stachyose on the human intestinal microbiota and metabolism.
33222240	4	51	theme	liquid	641:646	arg1	UHPLC-MS/MS					689:699	UHPLC-MS/MS	689:699	UHPLC-MS/MS	689:699	Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was used to study the effects of stachyose intake on the metabolites and metabolic pathways of the transplanted human intestinal microbiota.
33222240	4	51	theme	liquid	641:646	arg1	spectrometry					675:686	Ultra-high-performance liquid chromatography-tandem mass spectrometry	618:686	Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)	618:700	Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was used to study the effects of stachyose intake on the metabolites and metabolic pathways of the transplanted human intestinal microbiota.
33222240	4	52	theme	microbiota	831:840	arg1	pathways					785:792	metabolic pathways	775:792	metabolic pathways	775:792	Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was used to study the effects of stachyose intake on the metabolites and metabolic pathways of the transplanted human intestinal microbiota.
33222240	4	52	theme	microbiota	831:840	arg1	metabolites					759:769	metabolites	759:769	metabolites	759:769	Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was used to study the effects of stachyose intake on the metabolites and metabolic pathways of the transplanted human intestinal microbiota.
33222240	2	53	theme	human	341:345	arg1	microbiota					358:367	the human intestinal microbiota	337:367	the human intestinal microbiota	337:367	However, few studies have investigated the long-term effects of stachyose on the human intestinal microbiota and metabolism.
33222240	2	54	from	effects	313:319	arg1	microbiota					358:367	the human intestinal microbiota	337:367	the human intestinal microbiota	337:367	However, few studies have investigated the long-term effects of stachyose on the human intestinal microbiota and metabolism.
33222240	2	54	from	effects	313:319	arg1	metabolism					373:382	metabolism	373:382	metabolism	373:382	However, few studies have investigated the long-term effects of stachyose on the human intestinal microbiota and metabolism.
33222240	4	55	theme	Ultra-high-performance	618:639	arg1	UHPLC-MS/MS					689:699	UHPLC-MS/MS	689:699	UHPLC-MS/MS	689:699	Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was used to study the effects of stachyose intake on the metabolites and metabolic pathways of the transplanted human intestinal microbiota.
33222240	4	55	theme	Ultra-high-performance	618:639	arg1	spectrometry					675:686	Ultra-high-performance liquid chromatography-tandem mass spectrometry	618:686	Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)	618:700	Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was used to study the effects of stachyose intake on the metabolites and metabolic pathways of the transplanted human intestinal microbiota.
33222240	0	56	theme	fecal	71:75	arg1	microbiota					77:86	fecal microbiota	71:86	fecal microbiota	71:86	Microbiome-metabolomic analyses of the impacts of dietary stachyose on fecal microbiota and metabolites in infants intestinal microbiota-associated mice.
33222240	0	57	from	metabolites	92:102	arg1	infants					107:113	infants	107:113	infants	107:113	Microbiome-metabolomic analyses of the impacts of dietary stachyose on fecal microbiota and metabolites in infants intestinal microbiota-associated mice.
33222240	2	58	theme	few	269:271	arg1	studies					273:279	few studies	269:279	few studies	269:279	However, few studies have investigated the long-term effects of stachyose on the human intestinal microbiota and metabolism.
33222240	3	59	theme	intestinal	519:528	arg1	metabolism					530:539	intestinal metabolism	519:539	intestinal metabolism	519:539	Therefore, in this study, the feces of infants were transplanted into germ-free mice, and the effect of long-term stachyose intake on intestinal metabolism was examined by comparing the results of microbiome and metabolome analyses.
33222240	5	60	theme	intestinal	923:932	arg1	microbiota					934:943	the intestinal microbiota	919:943	the intestinal microbiota	919:943	RESULTS We observed that stachyose significantly altered the composition of the intestinal microbiota and metabolites, up-regulated production of the metabolite taurocholic acid, down-regulated amino acid metabolism, and significantly regulated the metabolism of taurine and hydroxytaurine, pantothenate and coenzyme A (CoA) biosynthesis, and other signaling pathways.
33222240	4	61	theme	mass	670:673	arg1	UHPLC-MS/MS					689:699	UHPLC-MS/MS	689:699	UHPLC-MS/MS	689:699	Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was used to study the effects of stachyose intake on the metabolites and metabolic pathways of the transplanted human intestinal microbiota.
33222240	4	61	theme	mass	670:673	arg1	spectrometry					675:686	Ultra-high-performance liquid chromatography-tandem mass spectrometry	618:686	Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)	618:700	Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was used to study the effects of stachyose intake on the metabolites and metabolic pathways of the transplanted human intestinal microbiota.
33222240	5	62	theme	signaling	1192:1200	arg1	pathways					1202:1209	other signaling pathways	1186:1209	other signaling pathways	1186:1209	RESULTS We observed that stachyose significantly altered the composition of the intestinal microbiota and metabolites, up-regulated production of the metabolite taurocholic acid, down-regulated amino acid metabolism, and significantly regulated the metabolism of taurine and hydroxytaurine, pantothenate and coenzyme A (CoA) biosynthesis, and other signaling pathways.
33222240	1	63	theme	intestinal	169:178	arg1	microbiota					180:189	The intestinal microbiota	165:189	The intestinal microbiota	165:189	BACKGROUND The intestinal microbiota and metabolites play an important role in human health and immunity.
33222240	4	64	theme	chromatography-tandem	648:668	arg1	UHPLC-MS/MS					689:699	UHPLC-MS/MS	689:699	UHPLC-MS/MS	689:699	Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was used to study the effects of stachyose intake on the metabolites and metabolic pathways of the transplanted human intestinal microbiota.
33222240	4	64	theme	chromatography-tandem	648:668	arg1	spectrometry					675:686	Ultra-high-performance liquid chromatography-tandem mass spectrometry	618:686	Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)	618:700	Ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was used to study the effects of stachyose intake on the metabolites and metabolic pathways of the transplanted human intestinal microbiota.
33090600	6	0	theme	monocyte-derived	1175:1190	arg1	DCs					1192:1194	human monocyte-derived DCs	1169:1194	human monocyte-derived DCs	1169:1194	Finally, a cytokine secretion assay was carried out on human monocyte-derived DCs, which showed the ability of the neoglycopeptide decorated with a single LeX to alter the balance of pro- and anti-inflammatory cytokines, inducing a tolerogenic response.
33090600	2	1	gly	N-glycosylation	311:325	arg1	Asn31					330:334	Asn31	330:334	Asn31 of MOG	330:341	Post-translational N-glycosylation of Asn31 of MOG seems to play a key role in modulating the immune response towards myelin.
33090600	2	2	theme	MOG	339:341	arg1	Asn31					330:334	Asn31	330:334	Asn31 of MOG	330:341	Post-translational N-glycosylation of Asn31 of MOG seems to play a key role in modulating the immune response towards myelin.
33090600	6	3	theme	pro-	1297:1300	arg1	cytokines					1324:1332	pro- and anti-inflammatory cytokines	1297:1332	pro- and anti-inflammatory cytokines	1297:1332	Finally, a cytokine secretion assay was carried out on human monocyte-derived DCs, which showed the ability of the neoglycopeptide decorated with a single LeX to alter the balance of pro- and anti-inflammatory cytokines, inducing a tolerogenic response.
33090600	1	4	theme	protein	154:160	arg1	glycoprotein					185:196	The protein myelin oligodendrocyte glycoprotein	150:196	The protein myelin oligodendrocyte glycoprotein (MOG)	150:202	The protein myelin oligodendrocyte glycoprotein (MOG) is a key component of myelin and an autoantigen in the disease multiple sclerosis (MS).
33090600	1	4	theme	protein	154:160	arg1	component					213:221	a key component	207:221	a key component of myelin	207:231	The protein myelin oligodendrocyte glycoprotein (MOG) is a key component of myelin and an autoantigen in the disease multiple sclerosis (MS).
33090600	1	4	theme	protein	154:160	arg1	MOG					199:201	MOG	199:201	MOG	199:201	The protein myelin oligodendrocyte glycoprotein (MOG) is a key component of myelin and an autoantigen in the disease multiple sclerosis (MS).
33090600	3	5	from	structures	475:484	arg1	N-glycan					493:500	the N-glycan	489:500	the N-glycan of MOG	489:507	This is mediated by the interaction of Lewis-type glycan structures in the N-glycan of MOG with the DC-SIGN receptor on dendritic cells (DCs).
33090600	3	6	theme	MOG	505:507	arg1	N-glycan					493:500	the N-glycan	489:500	the N-glycan of MOG	489:507	This is mediated by the interaction of Lewis-type glycan structures in the N-glycan of MOG with the DC-SIGN receptor on dendritic cells (DCs).
33090600	4	7	theme	SPPS=solid-phase	681:696	arg1	block					674:678	an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block	594:678	an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis)	594:715	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	4	7	theme	SPPS=solid-phase	681:696	arg1	synthesis					706:714	SPPS=solid-phase peptide synthesis	681:714	SPPS=solid-phase peptide synthesis	681:714	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	6	8	theme	human	1169:1173	arg1	DCs					1192:1194	human monocyte-derived DCs	1169:1194	human monocyte-derived DCs	1169:1194	Finally, a cytokine secretion assay was carried out on human monocyte-derived DCs, which showed the ability of the neoglycopeptide decorated with a single LeX to alter the balance of pro- and anti-inflammatory cytokines, inducing a tolerogenic response.
33090600	1	9	gly	glycoprotein	185:196	arg1	glycoprotein					185:196	The protein myelin oligodendrocyte glycoprotein	150:196	The protein myelin oligodendrocyte glycoprotein (MOG)	150:202	The protein myelin oligodendrocyte glycoprotein (MOG) is a key component of myelin and an autoantigen in the disease multiple sclerosis (MS).
33090600	1	9	gly	glycoprotein	185:196	arg1	component					213:221	a key component	207:221	a key component of myelin	207:231	The protein myelin oligodendrocyte glycoprotein (MOG) is a key component of myelin and an autoantigen in the disease multiple sclerosis (MS).
33090600	1	9	gly	glycoprotein	185:196	arg1	MOG					199:201	MOG	199:201	MOG	199:201	The protein myelin oligodendrocyte glycoprotein (MOG) is a key component of myelin and an autoantigen in the disease multiple sclerosis (MS).
33090600	3	10	theme	glycan	468:473	arg1	structures					475:484	Lewis-type glycan structures	457:484	Lewis-type glycan structures in the N-glycan of MOG	457:507	This is mediated by the interaction of Lewis-type glycan structures in the N-glycan of MOG with the DC-SIGN receptor on dendritic cells (DCs).
33090600	4	11	contain	containing	756:765	arg2	Fucα1-3GlcNAc					813:825	Fucα1-3GlcNAc	813:825	Fucα1-3GlcNAc	813:825	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	4	11	contain	containing	756:765	arg2	LacNAc					802:807	LacNAc	802:807	LacNAc	802:807	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	4	11	contain	containing	756:765	arg2	oligosaccharides					784:799	two LeX -derived oligosaccharides	767:799	two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc	767:825	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	4	11	contain	containing	756:765	arg1	blocks					749:754	asparagine building blocks	729:754	asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc	729:825	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	0	12	theme	Impact	100:105	arg1	Ligand					69:74	a Lewis X Type DC-SIGN Ligand	46:74	a Lewis X Type DC-SIGN Ligand	46:74	Synthesis of Asparagine Derivatives Harboring a Lewis X Type DC-SIGN Ligand and Evaluation of their Impact on Immunomodulation in Multiple Sclerosis.
33090600	0	12	theme	Impact	100:105	arg1	Evaluation					80:89	Evaluation	80:89	Evaluation of their Impact on Immunomodulation in Multiple Sclerosis	80:147	Synthesis of Asparagine Derivatives Harboring a Lewis X Type DC-SIGN Ligand and Evaluation of their Impact on Immunomodulation in Multiple Sclerosis.
33090600	4	13	theme	Fmoc-SPPS-compatible	633:652	arg1	block					674:678	an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block	594:678	an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis)	594:715	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	4	13	theme	Fmoc-SPPS-compatible	633:652	arg1	synthesis					706:714	SPPS=solid-phase peptide synthesis	681:714	SPPS=solid-phase peptide synthesis	681:714	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	1	14	theme	myelin	162:167	arg1	glycoprotein					185:196	The protein myelin oligodendrocyte glycoprotein	150:196	The protein myelin oligodendrocyte glycoprotein (MOG)	150:202	The protein myelin oligodendrocyte glycoprotein (MOG) is a key component of myelin and an autoantigen in the disease multiple sclerosis (MS).
33090600	1	14	theme	myelin	162:167	arg1	component					213:221	a key component	207:221	a key component of myelin	207:231	The protein myelin oligodendrocyte glycoprotein (MOG) is a key component of myelin and an autoantigen in the disease multiple sclerosis (MS).
33090600	1	14	theme	myelin	162:167	arg1	MOG					199:201	MOG	199:201	MOG	199:201	The protein myelin oligodendrocyte glycoprotein (MOG) is a key component of myelin and an autoantigen in the disease multiple sclerosis (MS).
33090600	4	15	link	-derived	775:782	arg1	Fucα1-3GlcNAc					813:825	Fucα1-3GlcNAc	813:825	Fucα1-3GlcNAc	813:825	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	4	15	link	-derived	775:782	arg1	LacNAc					802:807	LacNAc	802:807	LacNAc	802:807	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	4	15	link	-derived	775:782	arg1	oligosaccharides					784:799	two LeX -derived oligosaccharides	767:799	two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc	767:825	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	3	16	with	interaction	442:452	arg1	receptor					526:533	the DC-SIGN receptor	514:533	the DC-SIGN receptor on dendritic cells (DCs)	514:558	This is mediated by the interaction of Lewis-type glycan structures in the N-glycan of MOG with the DC-SIGN receptor on dendritic cells (DCs).
33090600	1	17	theme	oligodendrocyte	169:183	arg1	glycoprotein					185:196	The protein myelin oligodendrocyte glycoprotein	150:196	The protein myelin oligodendrocyte glycoprotein (MOG)	150:202	The protein myelin oligodendrocyte glycoprotein (MOG) is a key component of myelin and an autoantigen in the disease multiple sclerosis (MS).
33090600	1	17	theme	oligodendrocyte	169:183	arg1	component					213:221	a key component	207:221	a key component of myelin	207:231	The protein myelin oligodendrocyte glycoprotein (MOG) is a key component of myelin and an autoantigen in the disease multiple sclerosis (MS).
33090600	1	17	theme	oligodendrocyte	169:183	arg1	MOG					199:201	MOG	199:201	MOG	199:201	The protein myelin oligodendrocyte glycoprotein (MOG) is a key component of myelin and an autoantigen in the disease multiple sclerosis (MS).
33090600	3	18	from	interaction	442:452	arg1	N-glycan					493:500	the N-glycan	489:500	the N-glycan of MOG	489:507	This is mediated by the interaction of Lewis-type glycan structures in the N-glycan of MOG with the DC-SIGN receptor on dendritic cells (DCs).
33090600	5	19	theme	portion	904:910	arg1	glycosylation					868:880	the glycosylation	864:880	the glycosylation of the immunodominant portion of MOG (MOG31-55 )	864:929	These building blocks were used for the glycosylation of the immunodominant portion of MOG (MOG31-55 ) and analyzed with respect to their ability to bind to DC-SIGN in different biological setups, as well as their ability to inhibit the citrullination-induced aggregation of MOG31-55 .
33090600	4	20	theme	Lewis	607:611	arg1	LeX					616:618	LeX	616:618	LeX	616:618	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	4	20	theme	Lewis	607:611	arg1	X					613:613	an unnatural Lewis X	594:613	an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis)	594:715	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	6	21	theme	secretion	1134:1142	arg1	assay					1144:1148	a cytokine secretion assay	1123:1148	a cytokine secretion assay	1123:1148	Finally, a cytokine secretion assay was carried out on human monocyte-derived DCs, which showed the ability of the neoglycopeptide decorated with a single LeX to alter the balance of pro- and anti-inflammatory cytokines, inducing a tolerogenic response.
33090600	4	22	theme	asparagine	654:663	arg1	block					674:678	an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block	594:678	an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis)	594:715	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	4	22	theme	asparagine	654:663	arg1	synthesis					706:714	SPPS=solid-phase peptide synthesis	681:714	SPPS=solid-phase peptide synthesis	681:714	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	4	23	theme	block	674:678	arg1	synthesis					581:589	the synthesis	577:589	the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc	577:825	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	4	24	theme	unnatural	597:605	arg1	LeX					616:618	LeX	616:618	LeX	616:618	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	4	24	theme	unnatural	597:605	arg1	X					613:613	an unnatural Lewis X	594:613	an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis)	594:715	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	0	25	theme	Derivatives	24:34	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of Asparagine Derivatives Harboring a Lewis X Type DC-SIGN Ligand and Evaluation of their Impact on Immunomodulation in Multiple Sclerosis.	0:148	Synthesis of Asparagine Derivatives Harboring a Lewis X Type DC-SIGN Ligand and Evaluation of their Impact on Immunomodulation in Multiple Sclerosis.
33090600	1	26	theme	disease	259:265	arg1	MS					287:288	MS	287:288	MS	287:288	The protein myelin oligodendrocyte glycoprotein (MOG) is a key component of myelin and an autoantigen in the disease multiple sclerosis (MS).
33090600	1	26	theme	disease	259:265	arg1	sclerosis					276:284	the disease multiple sclerosis	255:284	the disease multiple sclerosis (MS)	255:289	The protein myelin oligodendrocyte glycoprotein (MOG) is a key component of myelin and an autoantigen in the disease multiple sclerosis (MS).
33090600	1	27	from	autoantigen	240:250	arg1	MS					287:288	MS	287:288	MS	287:288	The protein myelin oligodendrocyte glycoprotein (MOG) is a key component of myelin and an autoantigen in the disease multiple sclerosis (MS).
33090600	1	27	from	autoantigen	240:250	arg1	sclerosis					276:284	the disease multiple sclerosis	255:284	the disease multiple sclerosis (MS)	255:289	The protein myelin oligodendrocyte glycoprotein (MOG) is a key component of myelin and an autoantigen in the disease multiple sclerosis (MS).
33090600	4	28	theme	LeX	771:773	arg1	Fucα1-3GlcNAc					813:825	Fucα1-3GlcNAc	813:825	Fucα1-3GlcNAc	813:825	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	4	28	theme	LeX	771:773	arg1	LacNAc					802:807	LacNAc	802:807	LacNAc	802:807	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	4	28	theme	LeX	771:773	arg1	oligosaccharides					784:799	two LeX -derived oligosaccharides	767:799	two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc	767:825	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	6	29	link	monocyte-derived	1175:1190	arg1	DCs					1192:1194	human monocyte-derived DCs	1169:1194	human monocyte-derived DCs	1169:1194	Finally, a cytokine secretion assay was carried out on human monocyte-derived DCs, which showed the ability of the neoglycopeptide decorated with a single LeX to alter the balance of pro- and anti-inflammatory cytokines, inducing a tolerogenic response.
33090600	2	30	theme	immune	386:391	arg1	response					393:400	the immune response	382:400	the immune response towards myelin	382:415	Post-translational N-glycosylation of Asn31 of MOG seems to play a key role in modulating the immune response towards myelin.
33090600	0	31	theme	Asparagine	13:22	arg1	Derivatives					24:34	Asparagine Derivatives	13:34	Asparagine Derivatives Harboring a Lewis X Type DC-SIGN Ligand and Evaluation of their Impact on Immunomodulation in Multiple Sclerosis	13:147	Synthesis of Asparagine Derivatives Harboring a Lewis X Type DC-SIGN Ligand and Evaluation of their Impact on Immunomodulation in Multiple Sclerosis.
33090600	1	32	theme	multiple	267:274	arg1	MS					287:288	MS	287:288	MS	287:288	The protein myelin oligodendrocyte glycoprotein (MOG) is a key component of myelin and an autoantigen in the disease multiple sclerosis (MS).
33090600	1	32	theme	multiple	267:274	arg1	sclerosis					276:284	the disease multiple sclerosis	255:284	the disease multiple sclerosis (MS)	255:289	The protein myelin oligodendrocyte glycoprotein (MOG) is a key component of myelin and an autoantigen in the disease multiple sclerosis (MS).
33090600	5	33	theme	immunodominant	889:902	arg1	portion					904:910	the immunodominant portion	885:910	the immunodominant portion of MOG (MOG31-55 )	885:929	These building blocks were used for the glycosylation of the immunodominant portion of MOG (MOG31-55 ) and analyzed with respect to their ability to bind to DC-SIGN in different biological setups, as well as their ability to inhibit the citrullination-induced aggregation of MOG31-55 .
33090600	5	34	theme	different	996:1004	arg1	setups					1017:1022	different biological setups	996:1022	different biological setups	996:1022	These building blocks were used for the glycosylation of the immunodominant portion of MOG (MOG31-55 ) and analyzed with respect to their ability to bind to DC-SIGN in different biological setups, as well as their ability to inhibit the citrullination-induced aggregation of MOG31-55 .
33090600	3	35	theme	DC-SIGN	518:524	arg1	receptor					526:533	the DC-SIGN receptor	514:533	the DC-SIGN receptor on dendritic cells (DCs)	514:558	This is mediated by the interaction of Lewis-type glycan structures in the N-glycan of MOG with the DC-SIGN receptor on dendritic cells (DCs).
33090600	6	36	theme	tolerogenic	1346:1356	arg1	response					1358:1365	a tolerogenic response	1344:1365	a tolerogenic response	1344:1365	Finally, a cytokine secretion assay was carried out on human monocyte-derived DCs, which showed the ability of the neoglycopeptide decorated with a single LeX to alter the balance of pro- and anti-inflammatory cytokines, inducing a tolerogenic response.
33090600	4	37	theme	-containing	621:631	arg1	block					674:678	an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block	594:678	an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis)	594:715	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	4	37	theme	-containing	621:631	arg1	synthesis					706:714	SPPS=solid-phase peptide synthesis	681:714	SPPS=solid-phase peptide synthesis	681:714	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	0	38	theme	Multiple	130:137	arg1	Sclerosis					139:147	Multiple Sclerosis	130:147	Multiple Sclerosis	130:147	Synthesis of Asparagine Derivatives Harboring a Lewis X Type DC-SIGN Ligand and Evaluation of their Impact on Immunomodulation in Multiple Sclerosis.
33090600	0	39	from	Ligand	69:74	arg1	Immunomodulation					110:125	Immunomodulation	110:125	Immunomodulation in Multiple Sclerosis	110:147	Synthesis of Asparagine Derivatives Harboring a Lewis X Type DC-SIGN Ligand and Evaluation of their Impact on Immunomodulation in Multiple Sclerosis.
33090600	5	40	used	used	855:858	arg2	blocks					843:848	These building blocks	828:848	These building blocks	828:848	These building blocks were used for the glycosylation of the immunodominant portion of MOG (MOG31-55 ) and analyzed with respect to their ability to bind to DC-SIGN in different biological setups, as well as their ability to inhibit the citrullination-induced aggregation of MOG31-55 .
33090600	6	41	gly	neoglycopeptide	1229:1243	arg2	neoglycopeptide					1229:1243	the neoglycopeptide	1225:1243	the neoglycopeptide decorated with a single LeX	1225:1271	Finally, a cytokine secretion assay was carried out on human monocyte-derived DCs, which showed the ability of the neoglycopeptide decorated with a single LeX to alter the balance of pro- and anti-inflammatory cytokines, inducing a tolerogenic response.
33090600	6	42	theme	cytokines	1324:1332	arg1	balance					1286:1292	the balance	1282:1292	the balance of pro- and anti-inflammatory cytokines	1282:1332	Finally, a cytokine secretion assay was carried out on human monocyte-derived DCs, which showed the ability of the neoglycopeptide decorated with a single LeX to alter the balance of pro- and anti-inflammatory cytokines, inducing a tolerogenic response.
33090600	5	43	theme	citrullination-induced	1065:1086	arg1	aggregation					1088:1098	the citrullination-induced aggregation	1061:1098	the citrullination-induced aggregation of MOG31-55	1061:1110	These building blocks were used for the glycosylation of the immunodominant portion of MOG (MOG31-55 ) and analyzed with respect to their ability to bind to DC-SIGN in different biological setups, as well as their ability to inhibit the citrullination-induced aggregation of MOG31-55 .
33090600	5	44	gly	glycosylation	868:880	arg1	portion					904:910	the immunodominant portion	885:910	the immunodominant portion of MOG (MOG31-55 )	885:929	These building blocks were used for the glycosylation of the immunodominant portion of MOG (MOG31-55 ) and analyzed with respect to their ability to bind to DC-SIGN in different biological setups, as well as their ability to inhibit the citrullination-induced aggregation of MOG31-55 .
33090600	6	45	theme	single	1262:1267	arg1	LeX					1269:1271	a single LeX	1260:1271	a single LeX	1260:1271	Finally, a cytokine secretion assay was carried out on human monocyte-derived DCs, which showed the ability of the neoglycopeptide decorated with a single LeX to alter the balance of pro- and anti-inflammatory cytokines, inducing a tolerogenic response.
33090600	4	46	theme	blocks	749:754	arg1	synthesis					581:589	the synthesis	577:589	the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc	577:825	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	0	47	theme	X	54:54	arg1	Ligand					69:74	a Lewis X Type DC-SIGN Ligand	46:74	a Lewis X Type DC-SIGN Ligand	46:74	Synthesis of Asparagine Derivatives Harboring a Lewis X Type DC-SIGN Ligand and Evaluation of their Impact on Immunomodulation in Multiple Sclerosis.
33090600	6	48	theme	anti-inflammatory	1306:1322	arg1	cytokines					1324:1332	pro- and anti-inflammatory cytokines	1297:1332	pro- and anti-inflammatory cytokines	1297:1332	Finally, a cytokine secretion assay was carried out on human monocyte-derived DCs, which showed the ability of the neoglycopeptide decorated with a single LeX to alter the balance of pro- and anti-inflammatory cytokines, inducing a tolerogenic response.
33090600	3	49	theme	dendritic	538:546	arg1	DCs					555:557	DCs	555:557	DCs	555:557	This is mediated by the interaction of Lewis-type glycan structures in the N-glycan of MOG with the DC-SIGN receptor on dendritic cells (DCs).
33090600	3	49	theme	dendritic	538:546	arg1	cells					548:552	dendritic cells	538:552	dendritic cells (DCs)	538:558	This is mediated by the interaction of Lewis-type glycan structures in the N-glycan of MOG with the DC-SIGN receptor on dendritic cells (DCs).
33090600	2	50	theme	Asn31	330:334	arg1	N-glycosylation					311:325	Post-translational N-glycosylation	292:325	Post-translational N-glycosylation of Asn31 of MOG	292:341	Post-translational N-glycosylation of Asn31 of MOG seems to play a key role in modulating the immune response towards myelin.
33090600	0	51	theme	Lewis	48:52	arg1	Ligand					69:74	a Lewis X Type DC-SIGN Ligand	46:74	a Lewis X Type DC-SIGN Ligand	46:74	Synthesis of Asparagine Derivatives Harboring a Lewis X Type DC-SIGN Ligand and Evaluation of their Impact on Immunomodulation in Multiple Sclerosis.
33090600	0	52	from	Impact	100:105	arg1	Immunomodulation					110:125	Immunomodulation	110:125	Immunomodulation in Multiple Sclerosis	110:147	Synthesis of Asparagine Derivatives Harboring a Lewis X Type DC-SIGN Ligand and Evaluation of their Impact on Immunomodulation in Multiple Sclerosis.
33090600	4	53	theme	-derived	775:782	arg1	Fucα1-3GlcNAc					813:825	Fucα1-3GlcNAc	813:825	Fucα1-3GlcNAc	813:825	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	4	53	theme	-derived	775:782	arg1	LacNAc					802:807	LacNAc	802:807	LacNAc	802:807	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	4	53	theme	-derived	775:782	arg1	oligosaccharides					784:799	two LeX -derived oligosaccharides	767:799	two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc	767:825	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	3	54	theme	structures	475:484	arg1	interaction					442:452	the interaction	438:452	the interaction of Lewis-type glycan structures in the N-glycan of MOG with the DC-SIGN receptor on dendritic cells (DCs)	438:558	This is mediated by the interaction of Lewis-type glycan structures in the N-glycan of MOG with the DC-SIGN receptor on dendritic cells (DCs).
33090600	3	55	theme	Lewis-type	457:466	arg1	structures					475:484	Lewis-type glycan structures	457:484	Lewis-type glycan structures in the N-glycan of MOG	457:507	This is mediated by the interaction of Lewis-type glycan structures in the N-glycan of MOG with the DC-SIGN receptor on dendritic cells (DCs).
33090600	5	56	theme	MOG31-55	1103:1110	arg1	aggregation					1088:1098	the citrullination-induced aggregation	1061:1098	the citrullination-induced aggregation of MOG31-55	1061:1110	These building blocks were used for the glycosylation of the immunodominant portion of MOG (MOG31-55 ) and analyzed with respect to their ability to bind to DC-SIGN in different biological setups, as well as their ability to inhibit the citrullination-induced aggregation of MOG31-55 .
33090600	6	57	theme	neoglycopeptide	1229:1243	arg1	ability					1214:1220	the ability	1210:1220	the ability of the neoglycopeptide decorated with a single LeX to alter the balance of pro- and anti-inflammatory cytokines	1210:1332	Finally, a cytokine secretion assay was carried out on human monocyte-derived DCs, which showed the ability of the neoglycopeptide decorated with a single LeX to alter the balance of pro- and anti-inflammatory cytokines, inducing a tolerogenic response.
33090600	4	58	theme	building	665:672	arg1	block					674:678	an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block	594:678	an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis)	594:715	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	4	58	theme	building	665:672	arg1	synthesis					706:714	SPPS=solid-phase peptide synthesis	681:714	SPPS=solid-phase peptide synthesis	681:714	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	4	59	theme	peptide	698:704	arg1	block					674:678	an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block	594:678	an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis)	594:715	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	4	59	theme	peptide	698:704	arg1	synthesis					706:714	SPPS=solid-phase peptide synthesis	681:714	SPPS=solid-phase peptide synthesis	681:714	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	1	60	theme	key	209:211	arg1	glycoprotein					185:196	The protein myelin oligodendrocyte glycoprotein	150:196	The protein myelin oligodendrocyte glycoprotein (MOG)	150:202	The protein myelin oligodendrocyte glycoprotein (MOG) is a key component of myelin and an autoantigen in the disease multiple sclerosis (MS).
33090600	1	60	theme	key	209:211	arg1	component					213:221	a key component	207:221	a key component of myelin	207:231	The protein myelin oligodendrocyte glycoprotein (MOG) is a key component of myelin and an autoantigen in the disease multiple sclerosis (MS).
33090600	1	61	from	component	213:221	arg1	MS					287:288	MS	287:288	MS	287:288	The protein myelin oligodendrocyte glycoprotein (MOG) is a key component of myelin and an autoantigen in the disease multiple sclerosis (MS).
33090600	1	61	from	component	213:221	arg1	sclerosis					276:284	the disease multiple sclerosis	255:284	the disease multiple sclerosis (MS)	255:289	The protein myelin oligodendrocyte glycoprotein (MOG) is a key component of myelin and an autoantigen in the disease multiple sclerosis (MS).
33090600	4	62	dep	oligosaccharides	784:799	arg1	Fucα1-3GlcNAc					813:825	Fucα1-3GlcNAc	813:825	Fucα1-3GlcNAc	813:825	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	4	62	dep	oligosaccharides	784:799	arg1	LacNAc					802:807	LacNAc	802:807	LacNAc	802:807	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	4	62	dep	oligosaccharides	784:799	arg1	oligosaccharides					784:799	two LeX -derived oligosaccharides	767:799	two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc	767:825	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	5	63	theme	MOG	915:917	arg1	portion					904:910	the immunodominant portion	885:910	the immunodominant portion of MOG (MOG31-55 )	885:929	These building blocks were used for the glycosylation of the immunodominant portion of MOG (MOG31-55 ) and analyzed with respect to their ability to bind to DC-SIGN in different biological setups, as well as their ability to inhibit the citrullination-induced aggregation of MOG31-55 .
33090600	5	64	theme	biological	1006:1015	arg1	setups					1017:1022	different biological setups	996:1022	different biological setups	996:1022	These building blocks were used for the glycosylation of the immunodominant portion of MOG (MOG31-55 ) and analyzed with respect to their ability to bind to DC-SIGN in different biological setups, as well as their ability to inhibit the citrullination-induced aggregation of MOG31-55 .
33090600	2	65	theme	Post-translational	292:309	arg1	N-glycosylation					311:325	Post-translational N-glycosylation	292:325	Post-translational N-glycosylation of Asn31 of MOG	292:341	Post-translational N-glycosylation of Asn31 of MOG seems to play a key role in modulating the immune response towards myelin.
33090600	3	66	from	receptor	526:533	arg1	DCs					555:557	DCs	555:557	DCs	555:557	This is mediated by the interaction of Lewis-type glycan structures in the N-glycan of MOG with the DC-SIGN receptor on dendritic cells (DCs).
33090600	3	66	from	receptor	526:533	arg1	cells					548:552	dendritic cells	538:552	dendritic cells (DCs)	538:558	This is mediated by the interaction of Lewis-type glycan structures in the N-glycan of MOG with the DC-SIGN receptor on dendritic cells (DCs).
33090600	6	67	theme	cytokine	1125:1132	arg1	assay					1144:1148	a cytokine secretion assay	1123:1148	a cytokine secretion assay	1123:1148	Finally, a cytokine secretion assay was carried out on human monocyte-derived DCs, which showed the ability of the neoglycopeptide decorated with a single LeX to alter the balance of pro- and anti-inflammatory cytokines, inducing a tolerogenic response.
33090600	0	68	theme	DC-SIGN	61:67	arg1	Ligand					69:74	a Lewis X Type DC-SIGN Ligand	46:74	a Lewis X Type DC-SIGN Ligand	46:74	Synthesis of Asparagine Derivatives Harboring a Lewis X Type DC-SIGN Ligand and Evaluation of their Impact on Immunomodulation in Multiple Sclerosis.
33090600	0	69	from	Evaluation	80:89	arg1	Immunomodulation					110:125	Immunomodulation	110:125	Immunomodulation in Multiple Sclerosis	110:147	Synthesis of Asparagine Derivatives Harboring a Lewis X Type DC-SIGN Ligand and Evaluation of their Impact on Immunomodulation in Multiple Sclerosis.
33090600	4	70	theme	building	740:747	arg1	blocks					749:754	asparagine building blocks	729:754	asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc	729:825	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	5	71	theme	building	834:841	arg1	blocks					843:848	These building blocks	828:848	These building blocks	828:848	These building blocks were used for the glycosylation of the immunodominant portion of MOG (MOG31-55 ) and analyzed with respect to their ability to bind to DC-SIGN in different biological setups, as well as their ability to inhibit the citrullination-induced aggregation of MOG31-55 .
33090600	2	72	theme	key	359:361	arg1	role					363:366	a key role	357:366	a key role	357:366	Post-translational N-glycosylation of Asn31 of MOG seems to play a key role in modulating the immune response towards myelin.
33090600	0	73	from	Immunomodulation	110:125	arg1	Ligand					69:74	a Lewis X Type DC-SIGN Ligand	46:74	a Lewis X Type DC-SIGN Ligand	46:74	Synthesis of Asparagine Derivatives Harboring a Lewis X Type DC-SIGN Ligand and Evaluation of their Impact on Immunomodulation in Multiple Sclerosis.
33090600	0	73	from	Immunomodulation	110:125	arg1	Sclerosis					139:147	Multiple Sclerosis	130:147	Multiple Sclerosis	130:147	Synthesis of Asparagine Derivatives Harboring a Lewis X Type DC-SIGN Ligand and Evaluation of their Impact on Immunomodulation in Multiple Sclerosis.
33090600	0	73	from	Immunomodulation	110:125	arg1	Evaluation					80:89	Evaluation	80:89	Evaluation of their Impact on Immunomodulation in Multiple Sclerosis	80:147	Synthesis of Asparagine Derivatives Harboring a Lewis X Type DC-SIGN Ligand and Evaluation of their Impact on Immunomodulation in Multiple Sclerosis.
33090600	0	74	theme	Type	56:59	arg1	Ligand					69:74	a Lewis X Type DC-SIGN Ligand	46:74	a Lewis X Type DC-SIGN Ligand	46:74	Synthesis of Asparagine Derivatives Harboring a Lewis X Type DC-SIGN Ligand and Evaluation of their Impact on Immunomodulation in Multiple Sclerosis.
33090600	4	75	theme	asparagine	729:738	arg1	blocks					749:754	asparagine building blocks	729:754	asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc	729:825	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	4	76	theme	X	613:613	arg1	block					674:678	an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block	594:678	an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis)	594:715	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	4	76	theme	X	613:613	arg1	synthesis					706:714	SPPS=solid-phase peptide synthesis	681:714	SPPS=solid-phase peptide synthesis	681:714	Here, we report the synthesis of an unnatural Lewis X (LeX )-containing Fmoc-SPPS-compatible asparagine building block (SPPS=solid-phase peptide synthesis), as well as asparagine building blocks containing two LeX -derived oligosaccharides: LacNAc and Fucα1-3GlcNAc.
33090600	3	77	from	N-glycan	493:500	arg1	interaction					442:452	the interaction	438:452	the interaction of Lewis-type glycan structures in the N-glycan of MOG with the DC-SIGN receptor on dendritic cells (DCs)	438:558	This is mediated by the interaction of Lewis-type glycan structures in the N-glycan of MOG with the DC-SIGN receptor on dendritic cells (DCs).
33090600	1	78	theme	myelin	226:231	arg1	glycoprotein					185:196	The protein myelin oligodendrocyte glycoprotein	150:196	The protein myelin oligodendrocyte glycoprotein (MOG)	150:202	The protein myelin oligodendrocyte glycoprotein (MOG) is a key component of myelin and an autoantigen in the disease multiple sclerosis (MS).
33090600	1	78	theme	myelin	226:231	arg1	autoantigen					240:250	an autoantigen	237:250	an autoantigen in the disease multiple sclerosis (MS)	237:289	The protein myelin oligodendrocyte glycoprotein (MOG) is a key component of myelin and an autoantigen in the disease multiple sclerosis (MS).
33090600	1	78	theme	myelin	226:231	arg1	component					213:221	a key component	207:221	a key component of myelin	207:231	The protein myelin oligodendrocyte glycoprotein (MOG) is a key component of myelin and an autoantigen in the disease multiple sclerosis (MS).
32125479	7	0	theme	commensal	1204:1212	arg1	Prevotella					1249:1258	Prevotella	1249:1258	Prevotella	1249:1258	Of note, PD supplementation decreased the relative abundance of pathogenic organisms, including Defluviicoccus and Gardnerella, while markedly increasing that of commensal bacteria (genera Psychrobacter and Prevotella), which have important roles in nutrient absorption and immune response regulation.
32125479	7	0	theme	commensal	1204:1212	arg1	bacteria					1214:1221	commensal bacteria	1204:1221	commensal bacteria (genera Psychrobacter and Prevotella)	1204:1259	Of note, PD supplementation decreased the relative abundance of pathogenic organisms, including Defluviicoccus and Gardnerella, while markedly increasing that of commensal bacteria (genera Psychrobacter and Prevotella), which have important roles in nutrient absorption and immune response regulation.
32125479	7	0	theme	commensal	1204:1212	arg1	Psychrobacter					1231:1243	Psychrobacter	1231:1243	Psychrobacter	1231:1243	Of note, PD supplementation decreased the relative abundance of pathogenic organisms, including Defluviicoccus and Gardnerella, while markedly increasing that of commensal bacteria (genera Psychrobacter and Prevotella), which have important roles in nutrient absorption and immune response regulation.
32125479	10	1	from	effects	1675:1681	arg1	piglets					1792:1798	early weaned piglets	1779:1798	early weaned piglets	1779:1798	Overall, this study provides a more comprehensive understanding of the effects of PD supplementation on the fecal microbial community and the modulation of SCFA production in early weaned piglets, thus indicating that PD can be used to alleviate weaning stress in piglets.
32125479	10	1	from	effects	1675:1681	arg1	community					1728:1736	the fecal microbial community	1708:1736	the fecal microbial community	1708:1736	Overall, this study provides a more comprehensive understanding of the effects of PD supplementation on the fecal microbial community and the modulation of SCFA production in early weaned piglets, thus indicating that PD can be used to alleviate weaning stress in piglets.
32125479	2	2	contain	have	254:257	arg1	PD					245:246	PD	245:246	PD	245:246	We hypothesized that PD might have prebiotic potential affecting the microbiota composition, because it contains a non-digestible portion that may behave as dietary fiber.
32125479	2	2	contain	have	254:257	arg2	potential					269:277	prebiotic potential	259:277	prebiotic potential	259:277	We hypothesized that PD might have prebiotic potential affecting the microbiota composition, because it contains a non-digestible portion that may behave as dietary fiber.
32125479	4	3	theme	PD	637:638	arg1	groups					650:655	the PD (treated) groups	633:655	the PD (treated) groups	633:655	The piglets in the PD (treated) groups showed greater antioxidant capacity and feed efficiency (P < 0.05), as well as improved intestinal morphology in comparison with the piglets in the weaned (control) group.
32125479	2	4	theme	prebiotic	259:267	arg1	potential					269:277	prebiotic potential	259:277	prebiotic potential	259:277	We hypothesized that PD might have prebiotic potential affecting the microbiota composition, because it contains a non-digestible portion that may behave as dietary fiber.
32125479	4	5	theme	treated	641:647	arg1	groups					650:655	the PD (treated) groups	633:655	the PD (treated) groups	633:655	The piglets in the PD (treated) groups showed greater antioxidant capacity and feed efficiency (P < 0.05), as well as improved intestinal morphology in comparison with the piglets in the weaned (control) group.
32125479	3	6	theme	weaned	557:562	arg1	piglets					564:570	weaned piglets	557:570	weaned piglets receiving dietary supplementation of 0.5% PD	557:615	This study investigated the effects of PD supplementation on growth performance, gut morphology, short-chain fatty acids (SCFAs), and the bacterial community in weaned piglets receiving dietary supplementation of 0.5% PD.
32125479	2	7	theme	microbiota	293:302	arg1	composition					304:314	the microbiota composition	289:314	the microbiota composition	289:314	We hypothesized that PD might have prebiotic potential affecting the microbiota composition, because it contains a non-digestible portion that may behave as dietary fiber.
32125479	4	8	from	piglets	790:796	arg1	group					822:826	the weaned (control) group	801:826	the weaned (control) group	801:826	The piglets in the PD (treated) groups showed greater antioxidant capacity and feed efficiency (P < 0.05), as well as improved intestinal morphology in comparison with the piglets in the weaned (control) group.
32125479	8	9	theme	SCFAs	1423:1427	arg1	production					1409:1418	increased production	1399:1418	increased production of SCFAs	1399:1427	The most notable effect in the PD treatment groups was increased production of SCFAs in the feces of PD-treated weaned piglets.
32125479	8	9	theme	SCFAs	1423:1427	arg1	effect					1361:1366	The most notable effect	1344:1366	The most notable effect in the PD treatment groups	1344:1393	The most notable effect in the PD treatment groups was increased production of SCFAs in the feces of PD-treated weaned piglets.
32125479	10	10	theme	early	1779:1783	arg1	piglets					1792:1798	early weaned piglets	1779:1798	early weaned piglets	1779:1798	Overall, this study provides a more comprehensive understanding of the effects of PD supplementation on the fecal microbial community and the modulation of SCFA production in early weaned piglets, thus indicating that PD can be used to alleviate weaning stress in piglets.
32125479	7	11	theme	response	1323:1330	arg1	regulation					1332:1341	immune response regulation	1316:1341	immune response regulation	1316:1341	Of note, PD supplementation decreased the relative abundance of pathogenic organisms, including Defluviicoccus and Gardnerella, while markedly increasing that of commensal bacteria (genera Psychrobacter and Prevotella), which have important roles in nutrient absorption and immune response regulation.
32125479	5	12	dep	contents	908:915	arg1	the					898:900	the	898:900	the	898:900	Gut microbiota profiles were assessed through 16S rRNA sequencing on the ileum contents and feces of early weaned piglets.
32125479	3	13	from	community	544:552	arg1	piglets					564:570	weaned piglets	557:570	weaned piglets receiving dietary supplementation of 0.5% PD	557:615	This study investigated the effects of PD supplementation on growth performance, gut morphology, short-chain fatty acids (SCFAs), and the bacterial community in weaned piglets receiving dietary supplementation of 0.5% PD.
32125479	3	14	theme	PD	614:615	arg1	supplementation					590:604	dietary supplementation	582:604	dietary supplementation of 0.5% PD	582:615	This study investigated the effects of PD supplementation on growth performance, gut morphology, short-chain fatty acids (SCFAs), and the bacterial community in weaned piglets receiving dietary supplementation of 0.5% PD.
32125479	9	15	theme	Correlation	1472:1482	arg1	analysis					1484:1491	Correlation analysis	1472:1491	Correlation analysis	1472:1491	Correlation analysis revealed that the improvement in SCFAs was positively correlated with the increase in SCFA-producing bacteria.
32125479	10	16	from	modulation	1746:1755	arg1	piglets					1792:1798	early weaned piglets	1779:1798	early weaned piglets	1779:1798	Overall, this study provides a more comprehensive understanding of the effects of PD supplementation on the fecal microbial community and the modulation of SCFA production in early weaned piglets, thus indicating that PD can be used to alleviate weaning stress in piglets.
32125479	10	16	from	modulation	1746:1755	arg1	community					1728:1736	the fecal microbial community	1708:1736	the fecal microbial community	1708:1736	Overall, this study provides a more comprehensive understanding of the effects of PD supplementation on the fecal microbial community and the modulation of SCFA production in early weaned piglets, thus indicating that PD can be used to alleviate weaning stress in piglets.
32125479	10	17	from	community	1728:1736	arg1	understanding					1654:1666	a more comprehensive understanding	1633:1666	a more comprehensive understanding of the effects of PD supplementation on the fecal microbial community and the modulation of SCFA production in early weaned piglets	1633:1798	Overall, this study provides a more comprehensive understanding of the effects of PD supplementation on the fecal microbial community and the modulation of SCFA production in early weaned piglets, thus indicating that PD can be used to alleviate weaning stress in piglets.
32125479	0	18	theme	microbiota	73:82	arg1	composition					84:94	intestinal microbiota composition	62:94	intestinal microbiota composition	62:94	Pyrodextrin enhances intestinal function through changing the intestinal microbiota composition and metabolism in early weaned piglets.
32125479	3	19	theme	0.5	609:611	arg1	%					612:612	%	612:612	%	612:612	This study investigated the effects of PD supplementation on growth performance, gut morphology, short-chain fatty acids (SCFAs), and the bacterial community in weaned piglets receiving dietary supplementation of 0.5% PD.
32125479	5	20	theme	microbiota	833:842	arg1	profiles					844:851	Gut microbiota profiles	829:851	Gut microbiota profiles	829:851	Gut microbiota profiles were assessed through 16S rRNA sequencing on the ileum contents and feces of early weaned piglets.
32125479	6	21	theme	Several	952:958	arg1	enrichments					972:982	Several genus-level enrichments	952:982	Several genus-level enrichments	952:982	Several genus-level enrichments and depletions were observed in response to PD treatment.
32125479	8	22	theme	weaned	1456:1461	arg1	piglets					1463:1469	PD-treated weaned piglets	1445:1469	PD-treated weaned piglets	1445:1469	The most notable effect in the PD treatment groups was increased production of SCFAs in the feces of PD-treated weaned piglets.
32125479	4	23	theme	greater	664:670	arg1	capacity					684:691	greater antioxidant capacity	664:691	greater antioxidant capacity	664:691	The piglets in the PD (treated) groups showed greater antioxidant capacity and feed efficiency (P < 0.05), as well as improved intestinal morphology in comparison with the piglets in the weaned (control) group.
32125479	8	24	from	production	1409:1418	arg1	feces					1436:1440	the feces	1432:1440	the feces of PD-treated weaned piglets	1432:1469	The most notable effect in the PD treatment groups was increased production of SCFAs in the feces of PD-treated weaned piglets.
32125479	9	25	from	improvement	1511:1521	arg1	SCFAs					1526:1530	SCFAs	1526:1530	SCFAs	1526:1530	Correlation analysis revealed that the improvement in SCFAs was positively correlated with the increase in SCFA-producing bacteria.
32125479	5	26	theme	rRNA	879:882	arg1	sequencing					884:893	rRNA sequencing	879:893	rRNA sequencing	879:893	Gut microbiota profiles were assessed through 16S rRNA sequencing on the ileum contents and feces of early weaned piglets.
32125479	3	27	theme	PD	435:436	arg1	supplementation					438:452	PD supplementation	435:452	PD supplementation	435:452	This study investigated the effects of PD supplementation on growth performance, gut morphology, short-chain fatty acids (SCFAs), and the bacterial community in weaned piglets receiving dietary supplementation of 0.5% PD.
32125479	0	28	theme	weaned	120:125	arg1	piglets					127:133	early weaned piglets	114:133	early weaned piglets	114:133	Pyrodextrin enhances intestinal function through changing the intestinal microbiota composition and metabolism in early weaned piglets.
32125479	6	29	theme	PD	1028:1029	arg1	treatment					1031:1039	PD treatment	1028:1039	PD treatment	1028:1039	Several genus-level enrichments and depletions were observed in response to PD treatment.
32125479	2	30	theme	dietary	381:387	arg1	fiber					389:393	dietary fiber	381:393	dietary fiber	381:393	We hypothesized that PD might have prebiotic potential affecting the microbiota composition, because it contains a non-digestible portion that may behave as dietary fiber.
32125479	7	31	dep	bacteria	1214:1221	arg1	Prevotella					1249:1258	Prevotella	1249:1258	Prevotella	1249:1258	Of note, PD supplementation decreased the relative abundance of pathogenic organisms, including Defluviicoccus and Gardnerella, while markedly increasing that of commensal bacteria (genera Psychrobacter and Prevotella), which have important roles in nutrient absorption and immune response regulation.
32125479	7	31	dep	bacteria	1214:1221	arg1	bacteria					1214:1221	commensal bacteria	1204:1221	commensal bacteria (genera Psychrobacter and Prevotella)	1204:1259	Of note, PD supplementation decreased the relative abundance of pathogenic organisms, including Defluviicoccus and Gardnerella, while markedly increasing that of commensal bacteria (genera Psychrobacter and Prevotella), which have important roles in nutrient absorption and immune response regulation.
32125479	7	31	dep	bacteria	1214:1221	arg1	Psychrobacter					1231:1243	Psychrobacter	1231:1243	Psychrobacter	1231:1243	Of note, PD supplementation decreased the relative abundance of pathogenic organisms, including Defluviicoccus and Gardnerella, while markedly increasing that of commensal bacteria (genera Psychrobacter and Prevotella), which have important roles in nutrient absorption and immune response regulation.
32125479	8	32	theme	notable	1353:1359	arg1	effect					1361:1366	The most notable effect	1344:1366	The most notable effect in the PD treatment groups	1344:1393	The most notable effect in the PD treatment groups was increased production of SCFAs in the feces of PD-treated weaned piglets.
32125479	8	32	theme	notable	1353:1359	arg1	production					1409:1418	increased production	1399:1418	increased production of SCFAs	1399:1427	The most notable effect in the PD treatment groups was increased production of SCFAs in the feces of PD-treated weaned piglets.
32125479	0	33	theme	intestinal	21:30	arg1	function					32:39	intestinal function	21:39	intestinal function	21:39	Pyrodextrin enhances intestinal function through changing the intestinal microbiota composition and metabolism in early weaned piglets.
32125479	5	34	theme	ileum	902:906	arg1	contents					908:915	ileum contents	902:915	ileum contents	902:915	Gut microbiota profiles were assessed through 16S rRNA sequencing on the ileum contents and feces of early weaned piglets.
32125479	9	35	from	increase	1567:1574	arg1	bacteria					1594:1601	SCFA-producing bacteria	1579:1601	SCFA-producing bacteria	1579:1601	Correlation analysis revealed that the improvement in SCFAs was positively correlated with the increase in SCFA-producing bacteria.
32125479	7	36	theme	important	1273:1281	arg1	roles					1283:1287	important roles	1273:1287	important roles	1273:1287	Of note, PD supplementation decreased the relative abundance of pathogenic organisms, including Defluviicoccus and Gardnerella, while markedly increasing that of commensal bacteria (genera Psychrobacter and Prevotella), which have important roles in nutrient absorption and immune response regulation.
32125479	7	37	theme	pathogenic	1106:1115	arg1	Gardnerella					1157:1167	Gardnerella	1157:1167	Gardnerella	1157:1167	Of note, PD supplementation decreased the relative abundance of pathogenic organisms, including Defluviicoccus and Gardnerella, while markedly increasing that of commensal bacteria (genera Psychrobacter and Prevotella), which have important roles in nutrient absorption and immune response regulation.
32125479	7	37	theme	pathogenic	1106:1115	arg1	organisms					1117:1125	pathogenic organisms	1106:1125	pathogenic organisms	1106:1125	Of note, PD supplementation decreased the relative abundance of pathogenic organisms, including Defluviicoccus and Gardnerella, while markedly increasing that of commensal bacteria (genera Psychrobacter and Prevotella), which have important roles in nutrient absorption and immune response regulation.
32125479	7	37	theme	pathogenic	1106:1115	arg1	Defluviicoccus					1138:1151	Defluviicoccus	1138:1151	Defluviicoccus	1138:1151	Of note, PD supplementation decreased the relative abundance of pathogenic organisms, including Defluviicoccus and Gardnerella, while markedly increasing that of commensal bacteria (genera Psychrobacter and Prevotella), which have important roles in nutrient absorption and immune response regulation.
32125479	8	38	theme	PD	1375:1376	arg1	groups					1388:1393	the PD treatment groups	1371:1393	the PD treatment groups	1371:1393	The most notable effect in the PD treatment groups was increased production of SCFAs in the feces of PD-treated weaned piglets.
32125479	2	39	theme	non-digestible	339:352	arg1	portion					354:360	a non-digestible portion	337:360	a non-digestible portion that may behave as dietary fiber	337:393	We hypothesized that PD might have prebiotic potential affecting the microbiota composition, because it contains a non-digestible portion that may behave as dietary fiber.
32125479	10	40	used	used	1832:1835	arg2	PD					1822:1823	PD	1822:1823	PD	1822:1823	Overall, this study provides a more comprehensive understanding of the effects of PD supplementation on the fecal microbial community and the modulation of SCFA production in early weaned piglets, thus indicating that PD can be used to alleviate weaning stress in piglets.
32125479	6	41	located	observed	1004:1011	arg2	depletions					988:997	depletions	988:997	depletions	988:997	Several genus-level enrichments and depletions were observed in response to PD treatment.
32125479	6	41	located	observed	1004:1011	arg2	enrichments					972:982	Several genus-level enrichments	952:982	Several genus-level enrichments	952:982	Several genus-level enrichments and depletions were observed in response to PD treatment.
32125479	6	41	located	observed	1004:1011	arg1	response					1016:1023	response	1016:1023	response to PD treatment	1016:1039	Several genus-level enrichments and depletions were observed in response to PD treatment.
32125479	4	42	theme	intestinal	745:754	arg1	morphology					756:765	improved intestinal morphology	736:765	improved intestinal morphology	736:765	The piglets in the PD (treated) groups showed greater antioxidant capacity and feed efficiency (P < 0.05), as well as improved intestinal morphology in comparison with the piglets in the weaned (control) group.
32125479	3	43	theme	gut	477:479	arg1	morphology					481:490	gut morphology	477:490	gut morphology	477:490	This study investigated the effects of PD supplementation on growth performance, gut morphology, short-chain fatty acids (SCFAs), and the bacterial community in weaned piglets receiving dietary supplementation of 0.5% PD.
32125479	5	44	theme	weaned	936:941	arg1	piglets					943:949	early weaned piglets	930:949	early weaned piglets	930:949	Gut microbiota profiles were assessed through 16S rRNA sequencing on the ileum contents and feces of early weaned piglets.
32125479	10	45	from	understanding	1654:1666	arg1	piglets					1792:1798	early weaned piglets	1779:1798	early weaned piglets	1779:1798	Overall, this study provides a more comprehensive understanding of the effects of PD supplementation on the fecal microbial community and the modulation of SCFA production in early weaned piglets, thus indicating that PD can be used to alleviate weaning stress in piglets.
32125479	10	45	from	understanding	1654:1666	arg1	community					1728:1736	the fecal microbial community	1708:1736	the fecal microbial community	1708:1736	Overall, this study provides a more comprehensive understanding of the effects of PD supplementation on the fecal microbial community and the modulation of SCFA production in early weaned piglets, thus indicating that PD can be used to alleviate weaning stress in piglets.
32125479	10	46	theme	comprehensive	1640:1652	arg1	understanding					1654:1666	a more comprehensive understanding	1633:1666	a more comprehensive understanding of the effects of PD supplementation on the fecal microbial community and the modulation of SCFA production in early weaned piglets	1633:1798	Overall, this study provides a more comprehensive understanding of the effects of PD supplementation on the fecal microbial community and the modulation of SCFA production in early weaned piglets, thus indicating that PD can be used to alleviate weaning stress in piglets.
32125479	8	47	theme	increased	1399:1407	arg1	production					1409:1418	increased production	1399:1418	increased production of SCFAs	1399:1427	The most notable effect in the PD treatment groups was increased production of SCFAs in the feces of PD-treated weaned piglets.
32125479	8	47	theme	increased	1399:1407	arg1	effect					1361:1366	The most notable effect	1344:1366	The most notable effect in the PD treatment groups	1344:1393	The most notable effect in the PD treatment groups was increased production of SCFAs in the feces of PD-treated weaned piglets.
32125479	10	48	theme	microbial	1718:1726	arg1	community					1728:1736	the fecal microbial community	1708:1736	the fecal microbial community	1708:1736	Overall, this study provides a more comprehensive understanding of the effects of PD supplementation on the fecal microbial community and the modulation of SCFA production in early weaned piglets, thus indicating that PD can be used to alleviate weaning stress in piglets.
32125479	0	49	theme	intestinal	62:71	arg1	composition					84:94	intestinal microbiota composition	62:94	intestinal microbiota composition	62:94	Pyrodextrin enhances intestinal function through changing the intestinal microbiota composition and metabolism in early weaned piglets.
32125479	3	50	theme	fatty	505:509	arg1	acids					511:515	short-chain fatty acids	493:515	short-chain fatty acids (SCFAs)	493:523	This study investigated the effects of PD supplementation on growth performance, gut morphology, short-chain fatty acids (SCFAs), and the bacterial community in weaned piglets receiving dietary supplementation of 0.5% PD.
32125479	3	50	theme	fatty	505:509	arg1	SCFAs					518:522	SCFAs	518:522	SCFAs	518:522	This study investigated the effects of PD supplementation on growth performance, gut morphology, short-chain fatty acids (SCFAs), and the bacterial community in weaned piglets receiving dietary supplementation of 0.5% PD.
32125479	4	51	theme	control	813:819	arg1	group					822:826	the weaned (control) group	801:826	the weaned (control) group	801:826	The piglets in the PD (treated) groups showed greater antioxidant capacity and feed efficiency (P < 0.05), as well as improved intestinal morphology in comparison with the piglets in the weaned (control) group.
32125479	7	52	theme	PD	1051:1052	arg1	supplementation					1054:1068	PD supplementation	1051:1068	PD supplementation	1051:1068	Of note, PD supplementation decreased the relative abundance of pathogenic organisms, including Defluviicoccus and Gardnerella, while markedly increasing that of commensal bacteria (genera Psychrobacter and Prevotella), which have important roles in nutrient absorption and immune response regulation.
32125479	3	53	from	acids	511:515	arg1	piglets					564:570	weaned piglets	557:570	weaned piglets receiving dietary supplementation of 0.5% PD	557:615	This study investigated the effects of PD supplementation on growth performance, gut morphology, short-chain fatty acids (SCFAs), and the bacterial community in weaned piglets receiving dietary supplementation of 0.5% PD.
32125479	10	54	theme	modulation	1746:1755	arg1	understanding					1654:1666	a more comprehensive understanding	1633:1666	a more comprehensive understanding of the effects of PD supplementation on the fecal microbial community and the modulation of SCFA production in early weaned piglets	1633:1798	Overall, this study provides a more comprehensive understanding of the effects of PD supplementation on the fecal microbial community and the modulation of SCFA production in early weaned piglets, thus indicating that PD can be used to alleviate weaning stress in piglets.
32125479	10	55	theme	supplementation	1689:1703	arg1	effects					1675:1681	the effects	1671:1681	the effects of PD supplementation on the fecal microbial community	1671:1736	Overall, this study provides a more comprehensive understanding of the effects of PD supplementation on the fecal microbial community and the modulation of SCFA production in early weaned piglets, thus indicating that PD can be used to alleviate weaning stress in piglets.
32125479	10	55	theme	supplementation	1689:1703	arg1	modulation					1746:1755	the modulation	1742:1755	the modulation of SCFA production in early weaned piglets	1742:1798	Overall, this study provides a more comprehensive understanding of the effects of PD supplementation on the fecal microbial community and the modulation of SCFA production in early weaned piglets, thus indicating that PD can be used to alleviate weaning stress in piglets.
32125479	10	56	from	stress	1858:1863	arg1	piglets					1868:1874	piglets	1868:1874	piglets	1868:1874	Overall, this study provides a more comprehensive understanding of the effects of PD supplementation on the fecal microbial community and the modulation of SCFA production in early weaned piglets, thus indicating that PD can be used to alleviate weaning stress in piglets.
32125479	3	57	from	morphology	481:490	arg1	piglets					564:570	weaned piglets	557:570	weaned piglets receiving dietary supplementation of 0.5% PD	557:615	This study investigated the effects of PD supplementation on growth performance, gut morphology, short-chain fatty acids (SCFAs), and the bacterial community in weaned piglets receiving dietary supplementation of 0.5% PD.
32125479	8	58	from	effect	1361:1366	arg1	groups					1388:1393	the PD treatment groups	1371:1393	the PD treatment groups	1371:1393	The most notable effect in the PD treatment groups was increased production of SCFAs in the feces of PD-treated weaned piglets.
32125479	1	59	theme	heat	180:183	arg1	treatment					185:193	heat treatment	180:193	heat treatment	180:193	Pyrodextrin (PD) is prepared from starch by heat treatment and is resistant to amylase.
32125479	4	60	theme	weaned	805:810	arg1	group					822:826	the weaned (control) group	801:826	the weaned (control) group	801:826	The piglets in the PD (treated) groups showed greater antioxidant capacity and feed efficiency (P < 0.05), as well as improved intestinal morphology in comparison with the piglets in the weaned (control) group.
32125479	10	61	theme	SCFA	1760:1763	arg1	production					1765:1774	SCFA production	1760:1774	SCFA production	1760:1774	Overall, this study provides a more comprehensive understanding of the effects of PD supplementation on the fecal microbial community and the modulation of SCFA production in early weaned piglets, thus indicating that PD can be used to alleviate weaning stress in piglets.
32125479	4	62	from	piglets	622:628	arg1	groups					650:655	the PD (treated) groups	633:655	the PD (treated) groups	633:655	The piglets in the PD (treated) groups showed greater antioxidant capacity and feed efficiency (P < 0.05), as well as improved intestinal morphology in comparison with the piglets in the weaned (control) group.
32125479	10	63	theme	production	1765:1774	arg1	effects					1675:1681	the effects	1671:1681	the effects of PD supplementation on the fecal microbial community	1671:1736	Overall, this study provides a more comprehensive understanding of the effects of PD supplementation on the fecal microbial community and the modulation of SCFA production in early weaned piglets, thus indicating that PD can be used to alleviate weaning stress in piglets.
32125479	10	63	theme	production	1765:1774	arg1	modulation					1746:1755	the modulation	1742:1755	the modulation of SCFA production in early weaned piglets	1742:1798	Overall, this study provides a more comprehensive understanding of the effects of PD supplementation on the fecal microbial community and the modulation of SCFA production in early weaned piglets, thus indicating that PD can be used to alleviate weaning stress in piglets.
32125479	0	64	from	metabolism	100:109	arg1	piglets					127:133	early weaned piglets	114:133	early weaned piglets	114:133	Pyrodextrin enhances intestinal function through changing the intestinal microbiota composition and metabolism in early weaned piglets.
32125479	3	65	theme	short-chain	493:503	arg1	acids					511:515	short-chain fatty acids	493:515	short-chain fatty acids (SCFAs)	493:523	This study investigated the effects of PD supplementation on growth performance, gut morphology, short-chain fatty acids (SCFAs), and the bacterial community in weaned piglets receiving dietary supplementation of 0.5% PD.
32125479	3	65	theme	short-chain	493:503	arg1	SCFAs					518:522	SCFAs	518:522	SCFAs	518:522	This study investigated the effects of PD supplementation on growth performance, gut morphology, short-chain fatty acids (SCFAs), and the bacterial community in weaned piglets receiving dietary supplementation of 0.5% PD.
32125479	3	66	theme	dietary	582:588	arg1	supplementation					590:604	dietary supplementation	582:604	dietary supplementation of 0.5% PD	582:615	This study investigated the effects of PD supplementation on growth performance, gut morphology, short-chain fatty acids (SCFAs), and the bacterial community in weaned piglets receiving dietary supplementation of 0.5% PD.
32125479	0	67	from	composition	84:94	arg1	piglets					127:133	early weaned piglets	114:133	early weaned piglets	114:133	Pyrodextrin enhances intestinal function through changing the intestinal microbiota composition and metabolism in early weaned piglets.
32125479	7	68	contain	have	1268:1271	arg1	Prevotella					1249:1258	Prevotella	1249:1258	Prevotella	1249:1258	Of note, PD supplementation decreased the relative abundance of pathogenic organisms, including Defluviicoccus and Gardnerella, while markedly increasing that of commensal bacteria (genera Psychrobacter and Prevotella), which have important roles in nutrient absorption and immune response regulation.
32125479	7	68	contain	have	1268:1271	arg1	bacteria					1214:1221	commensal bacteria	1204:1221	commensal bacteria (genera Psychrobacter and Prevotella)	1204:1259	Of note, PD supplementation decreased the relative abundance of pathogenic organisms, including Defluviicoccus and Gardnerella, while markedly increasing that of commensal bacteria (genera Psychrobacter and Prevotella), which have important roles in nutrient absorption and immune response regulation.
32125479	7	68	contain	have	1268:1271	arg1	Psychrobacter					1231:1243	Psychrobacter	1231:1243	Psychrobacter	1231:1243	Of note, PD supplementation decreased the relative abundance of pathogenic organisms, including Defluviicoccus and Gardnerella, while markedly increasing that of commensal bacteria (genera Psychrobacter and Prevotella), which have important roles in nutrient absorption and immune response regulation.
32125479	7	68	contain	have	1268:1271	arg2	roles					1283:1287	important roles	1273:1287	important roles	1273:1287	Of note, PD supplementation decreased the relative abundance of pathogenic organisms, including Defluviicoccus and Gardnerella, while markedly increasing that of commensal bacteria (genera Psychrobacter and Prevotella), which have important roles in nutrient absorption and immune response regulation.
32125479	10	69	theme	weaned	1785:1790	arg1	piglets					1792:1798	early weaned piglets	1779:1798	early weaned piglets	1779:1798	Overall, this study provides a more comprehensive understanding of the effects of PD supplementation on the fecal microbial community and the modulation of SCFA production in early weaned piglets, thus indicating that PD can be used to alleviate weaning stress in piglets.
32125479	4	70	theme	antioxidant	672:682	arg1	capacity					684:691	greater antioxidant capacity	664:691	greater antioxidant capacity	664:691	The piglets in the PD (treated) groups showed greater antioxidant capacity and feed efficiency (P < 0.05), as well as improved intestinal morphology in comparison with the piglets in the weaned (control) group.
32125479	3	71	theme	%	612:612	arg1	PD					614:615	0.5% PD	609:615	0.5% PD	609:615	This study investigated the effects of PD supplementation on growth performance, gut morphology, short-chain fatty acids (SCFAs), and the bacterial community in weaned piglets receiving dietary supplementation of 0.5% PD.
32125479	3	72	theme	bacterial	534:542	arg1	community					544:552	the bacterial community	530:552	the bacterial community in weaned piglets receiving dietary supplementation of 0.5% PD	530:615	This study investigated the effects of PD supplementation on growth performance, gut morphology, short-chain fatty acids (SCFAs), and the bacterial community in weaned piglets receiving dietary supplementation of 0.5% PD.
32125479	8	73	theme	PD-treated	1445:1454	arg1	piglets					1463:1469	PD-treated weaned piglets	1445:1469	PD-treated weaned piglets	1445:1469	The most notable effect in the PD treatment groups was increased production of SCFAs in the feces of PD-treated weaned piglets.
32125479	5	74	theme	Gut	829:831	arg1	profiles					844:851	Gut microbiota profiles	829:851	Gut microbiota profiles	829:851	Gut microbiota profiles were assessed through 16S rRNA sequencing on the ileum contents and feces of early weaned piglets.
32125479	6	75	theme	genus-level	960:970	arg1	enrichments					972:982	Several genus-level enrichments	952:982	Several genus-level enrichments	952:982	Several genus-level enrichments and depletions were observed in response to PD treatment.
32125479	8	76	theme	piglets	1463:1469	arg1	feces					1436:1440	the feces	1432:1440	the feces of PD-treated weaned piglets	1432:1469	The most notable effect in the PD treatment groups was increased production of SCFAs in the feces of PD-treated weaned piglets.
32125479	7	77	theme	immune	1316:1321	arg1	regulation					1332:1341	immune response regulation	1316:1341	immune response regulation	1316:1341	Of note, PD supplementation decreased the relative abundance of pathogenic organisms, including Defluviicoccus and Gardnerella, while markedly increasing that of commensal bacteria (genera Psychrobacter and Prevotella), which have important roles in nutrient absorption and immune response regulation.
32125479	4	78	with	comparison	770:779	arg1	piglets					790:796	the piglets	786:796	the piglets in the weaned (control) group	786:826	The piglets in the PD (treated) groups showed greater antioxidant capacity and feed efficiency (P < 0.05), as well as improved intestinal morphology in comparison with the piglets in the weaned (control) group.
32125479	2	79	contain	contains	328:335	arg1	it					325:326	it	325:326	it	325:326	We hypothesized that PD might have prebiotic potential affecting the microbiota composition, because it contains a non-digestible portion that may behave as dietary fiber.
32125479	2	79	contain	contains	328:335	arg2	portion					354:360	a non-digestible portion	337:360	a non-digestible portion that may behave as dietary fiber	337:393	We hypothesized that PD might have prebiotic potential affecting the microbiota composition, because it contains a non-digestible portion that may behave as dietary fiber.
32125479	0	80	theme	early	114:118	arg1	piglets					127:133	early weaned piglets	114:133	early weaned piglets	114:133	Pyrodextrin enhances intestinal function through changing the intestinal microbiota composition and metabolism in early weaned piglets.
32125479	8	81	theme	treatment	1378:1386	arg1	groups					1388:1393	the PD treatment groups	1371:1393	the PD treatment groups	1371:1393	The most notable effect in the PD treatment groups was increased production of SCFAs in the feces of PD-treated weaned piglets.
32125479	7	82	theme	nutrient	1292:1299	arg1	absorption					1301:1310	nutrient absorption	1292:1310	nutrient absorption	1292:1310	Of note, PD supplementation decreased the relative abundance of pathogenic organisms, including Defluviicoccus and Gardnerella, while markedly increasing that of commensal bacteria (genera Psychrobacter and Prevotella), which have important roles in nutrient absorption and immune response regulation.
32125479	3	83	theme	supplementation	438:452	arg1	effects					424:430	the effects	420:430	the effects of PD supplementation on growth performance, gut morphology, short-chain fatty acids (SCFAs), and the bacterial community in weaned piglets receiving dietary supplementation of 0.5% PD	420:615	This study investigated the effects of PD supplementation on growth performance, gut morphology, short-chain fatty acids (SCFAs), and the bacterial community in weaned piglets receiving dietary supplementation of 0.5% PD.
32125479	10	84	from	piglets	1792:1798	arg1	understanding					1654:1666	a more comprehensive understanding	1633:1666	a more comprehensive understanding of the effects of PD supplementation on the fecal microbial community and the modulation of SCFA production in early weaned piglets	1633:1798	Overall, this study provides a more comprehensive understanding of the effects of PD supplementation on the fecal microbial community and the modulation of SCFA production in early weaned piglets, thus indicating that PD can be used to alleviate weaning stress in piglets.
32125479	7	85	theme	organisms	1117:1125	arg1	abundance					1093:1101	the relative abundance	1080:1101	the relative abundance of pathogenic organisms, including Defluviicoccus and Gardnerella	1080:1167	Of note, PD supplementation decreased the relative abundance of pathogenic organisms, including Defluviicoccus and Gardnerella, while markedly increasing that of commensal bacteria (genera Psychrobacter and Prevotella), which have important roles in nutrient absorption and immune response regulation.
32125479	0	86	dep	composition	84:94	arg1	the					58:60	the	58:60	the	58:60	Pyrodextrin enhances intestinal function through changing the intestinal microbiota composition and metabolism in early weaned piglets.
32125479	3	87	theme	growth	457:462	arg1	performance					464:474	growth performance	457:474	growth performance	457:474	This study investigated the effects of PD supplementation on growth performance, gut morphology, short-chain fatty acids (SCFAs), and the bacterial community in weaned piglets receiving dietary supplementation of 0.5% PD.
32125479	7	88	theme	relative	1084:1091	arg1	abundance					1093:1101	the relative abundance	1080:1101	the relative abundance of pathogenic organisms, including Defluviicoccus and Gardnerella	1080:1167	Of note, PD supplementation decreased the relative abundance of pathogenic organisms, including Defluviicoccus and Gardnerella, while markedly increasing that of commensal bacteria (genera Psychrobacter and Prevotella), which have important roles in nutrient absorption and immune response regulation.
32125479	9	89	theme	SCFA-producing	1579:1592	arg1	bacteria					1594:1601	SCFA-producing bacteria	1579:1601	SCFA-producing bacteria	1579:1601	Correlation analysis revealed that the improvement in SCFAs was positively correlated with the increase in SCFA-producing bacteria.
32125479	3	90	from	performance	464:474	arg1	piglets					564:570	weaned piglets	557:570	weaned piglets receiving dietary supplementation of 0.5% PD	557:615	This study investigated the effects of PD supplementation on growth performance, gut morphology, short-chain fatty acids (SCFAs), and the bacterial community in weaned piglets receiving dietary supplementation of 0.5% PD.
32125479	10	91	theme	PD	1686:1687	arg1	supplementation					1689:1703	PD supplementation	1686:1703	PD supplementation	1686:1703	Overall, this study provides a more comprehensive understanding of the effects of PD supplementation on the fecal microbial community and the modulation of SCFA production in early weaned piglets, thus indicating that PD can be used to alleviate weaning stress in piglets.
32125479	5	92	theme	early	930:934	arg1	piglets					943:949	early weaned piglets	930:949	early weaned piglets	930:949	Gut microbiota profiles were assessed through 16S rRNA sequencing on the ileum contents and feces of early weaned piglets.
32125479	10	93	theme	weaning	1850:1856	arg1	stress					1858:1863	weaning stress	1850:1863	weaning stress in piglets	1850:1874	Overall, this study provides a more comprehensive understanding of the effects of PD supplementation on the fecal microbial community and the modulation of SCFA production in early weaned piglets, thus indicating that PD can be used to alleviate weaning stress in piglets.
32125479	4	94	theme	improved	736:743	arg1	morphology					756:765	improved intestinal morphology	736:765	improved intestinal morphology	736:765	The piglets in the PD (treated) groups showed greater antioxidant capacity and feed efficiency (P < 0.05), as well as improved intestinal morphology in comparison with the piglets in the weaned (control) group.
32125479	3	95	from	effects	424:430	arg1	acids					511:515	short-chain fatty acids	493:515	short-chain fatty acids (SCFAs)	493:523	This study investigated the effects of PD supplementation on growth performance, gut morphology, short-chain fatty acids (SCFAs), and the bacterial community in weaned piglets receiving dietary supplementation of 0.5% PD.
32125479	3	95	from	effects	424:430	arg1	SCFAs					518:522	SCFAs	518:522	SCFAs	518:522	This study investigated the effects of PD supplementation on growth performance, gut morphology, short-chain fatty acids (SCFAs), and the bacterial community in weaned piglets receiving dietary supplementation of 0.5% PD.
32125479	3	95	from	effects	424:430	arg1	performance					464:474	growth performance	457:474	growth performance	457:474	This study investigated the effects of PD supplementation on growth performance, gut morphology, short-chain fatty acids (SCFAs), and the bacterial community in weaned piglets receiving dietary supplementation of 0.5% PD.
32125479	3	95	from	effects	424:430	arg1	morphology					481:490	gut morphology	477:490	gut morphology	477:490	This study investigated the effects of PD supplementation on growth performance, gut morphology, short-chain fatty acids (SCFAs), and the bacterial community in weaned piglets receiving dietary supplementation of 0.5% PD.
32125479	3	95	from	effects	424:430	arg1	community					544:552	the bacterial community	530:552	the bacterial community in weaned piglets receiving dietary supplementation of 0.5% PD	530:615	This study investigated the effects of PD supplementation on growth performance, gut morphology, short-chain fatty acids (SCFAs), and the bacterial community in weaned piglets receiving dietary supplementation of 0.5% PD.
32125479	10	96	theme	effects	1675:1681	arg1	understanding					1654:1666	a more comprehensive understanding	1633:1666	a more comprehensive understanding of the effects of PD supplementation on the fecal microbial community and the modulation of SCFA production in early weaned piglets	1633:1798	Overall, this study provides a more comprehensive understanding of the effects of PD supplementation on the fecal microbial community and the modulation of SCFA production in early weaned piglets, thus indicating that PD can be used to alleviate weaning stress in piglets.
32125479	5	97	theme	piglets	943:949	arg1	feces					921:925	feces	921:925	feces	921:925	Gut microbiota profiles were assessed through 16S rRNA sequencing on the ileum contents and feces of early weaned piglets.
32125479	5	97	theme	piglets	943:949	arg1	contents					908:915	ileum contents	902:915	ileum contents	902:915	Gut microbiota profiles were assessed through 16S rRNA sequencing on the ileum contents and feces of early weaned piglets.
32125479	10	98	theme	fecal	1712:1716	arg1	community					1728:1736	the fecal microbial community	1708:1736	the fecal microbial community	1708:1736	Overall, this study provides a more comprehensive understanding of the effects of PD supplementation on the fecal microbial community and the modulation of SCFA production in early weaned piglets, thus indicating that PD can be used to alleviate weaning stress in piglets.
32114163	8	0	theme	rRNA	973:976	arg1	sequencing					978:987	rRNA sequencing	973:987	rRNA sequencing	973:987	The alterations of fecal microflora were investigated by 16S rRNA sequencing.
32114163	1	1	theme	complex	148:154	arg1	weisan					132:137	ETHNOPHARMACOLOGICAL RELEVANCE Ping weisan	96:137	ETHNOPHARMACOLOGICAL RELEVANCE Ping weisan (PWS)	96:143	ETHNOPHARMACOLOGICAL RELEVANCE Ping weisan (PWS), a complex formulation used in traditional Chinese medicine, is first described in 1107 AD and published in the Prescriptions of Taiping Benevolent Dispensary.
32114163	1	1	theme	complex	148:154	arg1	formulation					156:166	a complex formulation	146:166	a complex formulation used in traditional Chinese medicine	146:203	ETHNOPHARMACOLOGICAL RELEVANCE Ping weisan (PWS), a complex formulation used in traditional Chinese medicine, is first described in 1107 AD and published in the Prescriptions of Taiping Benevolent Dispensary.
32114163	10	2	theme	MPO	1207:1209	arg1	activity					1211:1218	MPO activity	1207:1218	MPO activity	1207:1218	RESULTS PWS relieved DSS-induced loss of body weight, and improved stool consistency and MPO activity in mice.
32114163	10	3	theme	weight	1164:1169	arg1	loss					1151:1154	DSS-induced loss	1139:1154	DSS-induced loss of body weight	1139:1169	RESULTS PWS relieved DSS-induced loss of body weight, and improved stool consistency and MPO activity in mice.
32114163	0	4	theme	microbiota	72:81	arg1	composition					83:93	intestinal microbiota composition	61:93	intestinal microbiota composition	61:93	Ping weisan alleviates chronic colitis in mice by regulating intestinal microbiota composition.
32114163	14	5	theme	Firmicutes	1601:1610	arg1	abundance					1588:1596	the abundance	1584:1596	the abundance of Firmicutes in chronic UC mice induced by DSS	1584:1644	PWS treatment significantly decreased the abundance of Bacteroidetes, but increased the abundance of Firmicutes in chronic UC mice induced by DSS.
32114163	14	6	theme	PWS	1500:1502	arg1	treatment					1504:1512	PWS treatment	1500:1512	PWS treatment	1500:1512	PWS treatment significantly decreased the abundance of Bacteroidetes, but increased the abundance of Firmicutes in chronic UC mice induced by DSS.
32114163	9	7	theme	intestinal	1003:1012	arg1	occludin					1047:1054	intestinal tight junction protein including occludin	1003:1054	intestinal tight junction protein including occludin	1003:1054	Furthermore, intestinal tight junction protein including occludin, and serum lipopolysaccharide (LPS) level were also detected.
32114163	4	8	theme	MATERIALS	554:562	arg1	colitis					584:590	MATERIALS AND METHODS Chronic colitis	554:590	MATERIALS AND METHODS Chronic colitis	554:590	MATERIALS AND METHODS Chronic colitis was induced in C57BL/6 mice with 2.5% DSS in drinking water.
32114163	3	9	dep	aimed	446:450	arg1	AIM					426:428	AIM	426:428	AIM OF THE STUDY	426:441	AIM OF THE STUDY We aimed to examine whether PWS protects mice from chronic UC by regulating intestinal microbiota composition.
32114163	3	10	theme	microbiota	530:539	arg1	composition					541:551	intestinal microbiota composition	519:551	intestinal microbiota composition	519:551	AIM OF THE STUDY We aimed to examine whether PWS protects mice from chronic UC by regulating intestinal microbiota composition.
32114163	4	11	theme	Chronic	576:582	arg1	colitis					584:590	MATERIALS AND METHODS Chronic colitis	554:590	MATERIALS AND METHODS Chronic colitis	554:590	MATERIALS AND METHODS Chronic colitis was induced in C57BL/6 mice with 2.5% DSS in drinking water.
32114163	2	12	contain	has	343:345	arg1	PWS					339:341	PWS	339:341	PWS	339:341	We have previously confirmed that PWS has the effect of alleviating DSS-induced chronic ulcerative colitis (UC) in mice.
32114163	2	12	contain	has	343:345	arg2	effect					351:356	the effect	347:356	the effect of alleviating DSS-induced chronic ulcerative colitis (UC) in mice	347:423	We have previously confirmed that PWS has the effect of alleviating DSS-induced chronic ulcerative colitis (UC) in mice.
32114163	14	13	theme	Bacteroidetes	1555:1567	arg1	abundance					1542:1550	the abundance	1538:1550	the abundance of Bacteroidetes	1538:1567	PWS treatment significantly decreased the abundance of Bacteroidetes, but increased the abundance of Firmicutes in chronic UC mice induced by DSS.
32114163	8	14	theme	fecal	931:935	arg1	microflora					937:946	fecal microflora	931:946	fecal microflora	931:946	The alterations of fecal microflora were investigated by 16S rRNA sequencing.
32114163	9	15	theme	protein	1029:1035	arg1	occludin					1047:1054	intestinal tight junction protein including occludin	1003:1054	intestinal tight junction protein including occludin	1003:1054	Furthermore, intestinal tight junction protein including occludin, and serum lipopolysaccharide (LPS) level were also detected.
32114163	9	16	theme	junction	1020:1027	arg1	occludin					1047:1054	intestinal tight junction protein including occludin	1003:1054	intestinal tight junction protein including occludin	1003:1054	Furthermore, intestinal tight junction protein including occludin, and serum lipopolysaccharide (LPS) level were also detected.
32114163	4	17	theme	METHODS	568:574	arg1	colitis					584:590	MATERIALS AND METHODS Chronic colitis	554:590	MATERIALS AND METHODS Chronic colitis	554:590	MATERIALS AND METHODS Chronic colitis was induced in C57BL/6 mice with 2.5% DSS in drinking water.
32114163	15	18	theme	rebalancing	1742:1752	arg1	bacteria					1765:1772	rebalancing intestinal bacteria	1742:1772	rebalancing intestinal bacteria	1742:1772	CONCLUSIONS Combining with our previous results, we found that PWS could exert anti-UC role by rebalancing intestinal bacteria.
32114163	11	19	theme	IL-17A	1243:1248	arg1	levels					1233:1238	The levels	1229:1238	The levels of IL-17A and IFN-γ mRNA	1229:1263	The levels of IL-17A and IFN-γ mRNA were also reduced after treatment with PWS.
32114163	6	20	theme	myeloperoxidase	760:774	arg1	activity					782:789	myeloperoxidase (MPO) activity	760:789	myeloperoxidase (MPO) activity	760:789	Body weight changes, stool consistency and myeloperoxidase (MPO) activity were measured in these mice.
32114163	3	21	theme	chronic	494:500	arg1	UC					502:503	chronic UC	494:503	chronic UC	494:503	AIM OF THE STUDY We aimed to examine whether PWS protects mice from chronic UC by regulating intestinal microbiota composition.
32114163	1	22	theme	traditional	176:186	arg1	medicine					196:203	traditional Chinese medicine	176:203	traditional Chinese medicine	176:203	ETHNOPHARMACOLOGICAL RELEVANCE Ping weisan (PWS), a complex formulation used in traditional Chinese medicine, is first described in 1107 AD and published in the Prescriptions of Taiping Benevolent Dispensary.
32114163	2	23	theme	ulcerative	393:402	arg1	UC					413:414	UC	413:414	UC	413:414	We have previously confirmed that PWS has the effect of alleviating DSS-induced chronic ulcerative colitis (UC) in mice.
32114163	2	23	theme	ulcerative	393:402	arg1	colitis					404:410	DSS-induced chronic ulcerative colitis	373:410	DSS-induced chronic ulcerative colitis (UC) in mice	373:423	We have previously confirmed that PWS has the effect of alleviating DSS-induced chronic ulcerative colitis (UC) in mice.
32114163	12	24	theme	serum	1366:1370	arg1	LPS					1372:1374	serum LPS	1366:1374	serum LPS	1366:1374	PWS not only regulated occludin level but also decreased serum LPS.
32114163	12	25	theme	occludin	1332:1339	arg1	level					1341:1345	occludin level	1332:1345	occludin level	1332:1345	PWS not only regulated occludin level but also decreased serum LPS.
32114163	4	26	from	DSS	630:632	arg1	water					646:650	drinking water	637:650	drinking water	637:650	MATERIALS AND METHODS Chronic colitis was induced in C57BL/6 mice with 2.5% DSS in drinking water.
32114163	2	27	theme	chronic	385:391	arg1	UC					413:414	UC	413:414	UC	413:414	We have previously confirmed that PWS has the effect of alleviating DSS-induced chronic ulcerative colitis (UC) in mice.
32114163	2	27	theme	chronic	385:391	arg1	colitis					404:410	DSS-induced chronic ulcerative colitis	373:410	DSS-induced chronic ulcerative colitis (UC) in mice	373:423	We have previously confirmed that PWS has the effect of alleviating DSS-induced chronic ulcerative colitis (UC) in mice.
32114163	13	28	theme	microbial	1429:1437	arg1	composition					1439:1449	intestinal microbial composition	1418:1449	intestinal microbial composition	1418:1449	We further showed DSS-induced changes in intestinal microbial composition and richness are significantly regulated by PWS.
32114163	0	29	from	colitis	31:37	arg1	mice					42:45	mice	42:45	mice	42:45	Ping weisan alleviates chronic colitis in mice by regulating intestinal microbiota composition.
32114163	14	30	from	abundance	1588:1596	arg1	mice					1626:1629	chronic UC mice	1615:1629	chronic UC mice induced by DSS	1615:1644	PWS treatment significantly decreased the abundance of Bacteroidetes, but increased the abundance of Firmicutes in chronic UC mice induced by DSS.
32114163	8	31	theme	microflora	937:946	arg1	alterations					916:926	The alterations	912:926	The alterations of fecal microflora	912:946	The alterations of fecal microflora were investigated by 16S rRNA sequencing.
32114163	2	32	theme	DSS-induced	373:383	arg1	UC					413:414	UC	413:414	UC	413:414	We have previously confirmed that PWS has the effect of alleviating DSS-induced chronic ulcerative colitis (UC) in mice.
32114163	2	32	theme	DSS-induced	373:383	arg1	colitis					404:410	DSS-induced chronic ulcerative colitis	373:410	DSS-induced chronic ulcerative colitis (UC) in mice	373:423	We have previously confirmed that PWS has the effect of alleviating DSS-induced chronic ulcerative colitis (UC) in mice.
32114163	0	33	theme	Ping	0:3	arg1	weisan					5:10	Ping weisan	0:10	Ping weisan	0:10	Ping weisan alleviates chronic colitis in mice by regulating intestinal microbiota composition.
32114163	14	34	theme	UC	1623:1624	arg1	mice					1626:1629	chronic UC mice	1615:1629	chronic UC mice induced by DSS	1615:1644	PWS treatment significantly decreased the abundance of Bacteroidetes, but increased the abundance of Firmicutes in chronic UC mice induced by DSS.
32114163	6	35	theme	stool	738:742	arg1	consistency					744:754	stool consistency	738:754	stool consistency	738:754	Body weight changes, stool consistency and myeloperoxidase (MPO) activity were measured in these mice.
32114163	1	36	theme	ETHNOPHARMACOLOGICAL	96:115	arg1	PWS					140:142	PWS	140:142	PWS	140:142	ETHNOPHARMACOLOGICAL RELEVANCE Ping weisan (PWS), a complex formulation used in traditional Chinese medicine, is first described in 1107 AD and published in the Prescriptions of Taiping Benevolent Dispensary.
32114163	1	36	theme	ETHNOPHARMACOLOGICAL	96:115	arg1	weisan					132:137	ETHNOPHARMACOLOGICAL RELEVANCE Ping weisan	96:137	ETHNOPHARMACOLOGICAL RELEVANCE Ping weisan (PWS)	96:143	ETHNOPHARMACOLOGICAL RELEVANCE Ping weisan (PWS), a complex formulation used in traditional Chinese medicine, is first described in 1107 AD and published in the Prescriptions of Taiping Benevolent Dispensary.
32114163	1	36	theme	ETHNOPHARMACOLOGICAL	96:115	arg1	formulation					156:166	a complex formulation	146:166	a complex formulation used in traditional Chinese medicine	146:203	ETHNOPHARMACOLOGICAL RELEVANCE Ping weisan (PWS), a complex formulation used in traditional Chinese medicine, is first described in 1107 AD and published in the Prescriptions of Taiping Benevolent Dispensary.
32114163	9	37	theme	lipopolysaccharide	1067:1084	arg1	level					1092:1096	serum lipopolysaccharide (LPS) level	1061:1096	serum lipopolysaccharide (LPS) level	1061:1096	Furthermore, intestinal tight junction protein including occludin, and serum lipopolysaccharide (LPS) level were also detected.
32114163	1	38	theme	Chinese	188:194	arg1	medicine					196:203	traditional Chinese medicine	176:203	traditional Chinese medicine	176:203	ETHNOPHARMACOLOGICAL RELEVANCE Ping weisan (PWS), a complex formulation used in traditional Chinese medicine, is first described in 1107 AD and published in the Prescriptions of Taiping Benevolent Dispensary.
32114163	0	39	theme	chronic	23:29	arg1	colitis					31:37	chronic colitis	23:37	chronic colitis in mice	23:45	Ping weisan alleviates chronic colitis in mice by regulating intestinal microbiota composition.
32114163	7	40	theme	mRNA	874:877	arg1	levels					879:884	Interleukin-17A (IL-17A) and interferon gamma (IFN-γ) mRNA levels	820:884	Interleukin-17A (IL-17A) and interferon gamma (IFN-γ) mRNA levels	820:884	Interleukin-17A (IL-17A) and interferon gamma (IFN-γ) mRNA levels were detected by qRT-PCR.
32114163	1	41	theme	RELEVANCE	117:125	arg1	PWS					140:142	PWS	140:142	PWS	140:142	ETHNOPHARMACOLOGICAL RELEVANCE Ping weisan (PWS), a complex formulation used in traditional Chinese medicine, is first described in 1107 AD and published in the Prescriptions of Taiping Benevolent Dispensary.
32114163	1	41	theme	RELEVANCE	117:125	arg1	weisan					132:137	ETHNOPHARMACOLOGICAL RELEVANCE Ping weisan	96:137	ETHNOPHARMACOLOGICAL RELEVANCE Ping weisan (PWS)	96:143	ETHNOPHARMACOLOGICAL RELEVANCE Ping weisan (PWS), a complex formulation used in traditional Chinese medicine, is first described in 1107 AD and published in the Prescriptions of Taiping Benevolent Dispensary.
32114163	1	41	theme	RELEVANCE	117:125	arg1	formulation					156:166	a complex formulation	146:166	a complex formulation used in traditional Chinese medicine	146:203	ETHNOPHARMACOLOGICAL RELEVANCE Ping weisan (PWS), a complex formulation used in traditional Chinese medicine, is first described in 1107 AD and published in the Prescriptions of Taiping Benevolent Dispensary.
32114163	15	42	theme	anti-UC	1726:1732	arg1	role					1734:1737	anti-UC role	1726:1737	anti-UC role	1726:1737	CONCLUSIONS Combining with our previous results, we found that PWS could exert anti-UC role by rebalancing intestinal bacteria.
32114163	3	43	theme	STUDY	437:441	arg1	AIM					426:428	AIM	426:428	AIM OF THE STUDY	426:441	AIM OF THE STUDY We aimed to examine whether PWS protects mice from chronic UC by regulating intestinal microbiota composition.
32114163	11	44	theme	mRNA	1260:1263	arg1	levels					1233:1238	The levels	1229:1238	The levels of IL-17A and IFN-γ mRNA	1229:1263	The levels of IL-17A and IFN-γ mRNA were also reduced after treatment with PWS.
32114163	1	45	theme	Taiping	274:280	arg1	Dispensary					293:302	Taiping Benevolent Dispensary	274:302	Taiping Benevolent Dispensary	274:302	ETHNOPHARMACOLOGICAL RELEVANCE Ping weisan (PWS), a complex formulation used in traditional Chinese medicine, is first described in 1107 AD and published in the Prescriptions of Taiping Benevolent Dispensary.
32114163	11	46	with	treatment	1289:1297	arg1	PWS					1304:1306	PWS	1304:1306	PWS	1304:1306	The levels of IL-17A and IFN-γ mRNA were also reduced after treatment with PWS.
32114163	1	47	theme	Ping	127:130	arg1	PWS					140:142	PWS	140:142	PWS	140:142	ETHNOPHARMACOLOGICAL RELEVANCE Ping weisan (PWS), a complex formulation used in traditional Chinese medicine, is first described in 1107 AD and published in the Prescriptions of Taiping Benevolent Dispensary.
32114163	1	47	theme	Ping	127:130	arg1	weisan					132:137	ETHNOPHARMACOLOGICAL RELEVANCE Ping weisan	96:137	ETHNOPHARMACOLOGICAL RELEVANCE Ping weisan (PWS)	96:143	ETHNOPHARMACOLOGICAL RELEVANCE Ping weisan (PWS), a complex formulation used in traditional Chinese medicine, is first described in 1107 AD and published in the Prescriptions of Taiping Benevolent Dispensary.
32114163	1	47	theme	Ping	127:130	arg1	formulation					156:166	a complex formulation	146:166	a complex formulation used in traditional Chinese medicine	146:203	ETHNOPHARMACOLOGICAL RELEVANCE Ping weisan (PWS), a complex formulation used in traditional Chinese medicine, is first described in 1107 AD and published in the Prescriptions of Taiping Benevolent Dispensary.
32114163	9	48	theme	serum	1061:1065	arg1	LPS					1087:1089	LPS	1087:1089	LPS	1087:1089	Furthermore, intestinal tight junction protein including occludin, and serum lipopolysaccharide (LPS) level were also detected.
32114163	9	48	theme	serum	1061:1065	arg1	lipopolysaccharide					1067:1084	serum lipopolysaccharide	1061:1084	serum lipopolysaccharide (LPS) level	1061:1096	Furthermore, intestinal tight junction protein including occludin, and serum lipopolysaccharide (LPS) level were also detected.
32114163	4	49	theme	C57BL/6	607:613	arg1	mice					615:618	C57BL/6 mice	607:618	C57BL/6 mice	607:618	MATERIALS AND METHODS Chronic colitis was induced in C57BL/6 mice with 2.5% DSS in drinking water.
32114163	1	50	theme	Benevolent	282:291	arg1	Dispensary					293:302	Taiping Benevolent Dispensary	274:302	Taiping Benevolent Dispensary	274:302	ETHNOPHARMACOLOGICAL RELEVANCE Ping weisan (PWS), a complex formulation used in traditional Chinese medicine, is first described in 1107 AD and published in the Prescriptions of Taiping Benevolent Dispensary.
32114163	7	51	theme	gamma	860:864	arg1	levels					879:884	Interleukin-17A (IL-17A) and interferon gamma (IFN-γ) mRNA levels	820:884	Interleukin-17A (IL-17A) and interferon gamma (IFN-γ) mRNA levels	820:884	Interleukin-17A (IL-17A) and interferon gamma (IFN-γ) mRNA levels were detected by qRT-PCR.
32114163	13	52	theme	intestinal	1418:1427	arg1	composition					1439:1449	intestinal microbial composition	1418:1449	intestinal microbial composition	1418:1449	We further showed DSS-induced changes in intestinal microbial composition and richness are significantly regulated by PWS.
32114163	1	53	theme	Dispensary	293:302	arg1	Prescriptions					257:269	the Prescriptions	253:269	the Prescriptions of Taiping Benevolent Dispensary	253:302	ETHNOPHARMACOLOGICAL RELEVANCE Ping weisan (PWS), a complex formulation used in traditional Chinese medicine, is first described in 1107 AD and published in the Prescriptions of Taiping Benevolent Dispensary.
32114163	10	54	theme	body	1159:1162	arg1	weight					1164:1169	body weight	1159:1169	body weight	1159:1169	RESULTS PWS relieved DSS-induced loss of body weight, and improved stool consistency and MPO activity in mice.
32114163	11	55	theme	IFN-γ	1254:1258	arg1	mRNA					1260:1263	IFN-γ mRNA	1254:1263	IFN-γ mRNA	1254:1263	The levels of IL-17A and IFN-γ mRNA were also reduced after treatment with PWS.
32114163	13	56	from	changes	1407:1413	arg1	richness					1455:1462	richness	1455:1462	richness	1455:1462	We further showed DSS-induced changes in intestinal microbial composition and richness are significantly regulated by PWS.
32114163	13	56	from	changes	1407:1413	arg1	composition					1439:1449	intestinal microbial composition	1418:1449	intestinal microbial composition	1418:1449	We further showed DSS-induced changes in intestinal microbial composition and richness are significantly regulated by PWS.
32114163	4	57	theme	drinking	637:644	arg1	water					646:650	drinking water	637:650	drinking water	637:650	MATERIALS AND METHODS Chronic colitis was induced in C57BL/6 mice with 2.5% DSS in drinking water.
32114163	6	58	theme	weight	722:727	arg1	changes					729:735	Body weight changes	717:735	Body weight changes	717:735	Body weight changes, stool consistency and myeloperoxidase (MPO) activity were measured in these mice.
32114163	13	59	theme	DSS-induced	1395:1405	arg1	changes					1407:1413	DSS-induced changes	1395:1413	DSS-induced changes in intestinal microbial composition and richness	1395:1462	We further showed DSS-induced changes in intestinal microbial composition and richness are significantly regulated by PWS.
32114163	6	60	theme	Body	717:720	arg1	changes					729:735	Body weight changes	717:735	Body weight changes	717:735	Body weight changes, stool consistency and myeloperoxidase (MPO) activity were measured in these mice.
32114163	10	61	theme	stool	1185:1189	arg1	consistency					1191:1201	stool consistency	1185:1201	stool consistency	1185:1201	RESULTS PWS relieved DSS-induced loss of body weight, and improved stool consistency and MPO activity in mice.
32114163	3	62	theme	intestinal	519:528	arg1	composition					541:551	intestinal microbiota composition	519:551	intestinal microbiota composition	519:551	AIM OF THE STUDY We aimed to examine whether PWS protects mice from chronic UC by regulating intestinal microbiota composition.
32114163	10	63	theme	DSS-induced	1139:1149	arg1	loss					1151:1154	DSS-induced loss	1139:1154	DSS-induced loss of body weight	1139:1169	RESULTS PWS relieved DSS-induced loss of body weight, and improved stool consistency and MPO activity in mice.
32114163	7	64	theme	Interleukin-17A	820:834	arg1	levels					879:884	Interleukin-17A (IL-17A) and interferon gamma (IFN-γ) mRNA levels	820:884	Interleukin-17A (IL-17A) and interferon gamma (IFN-γ) mRNA levels	820:884	Interleukin-17A (IL-17A) and interferon gamma (IFN-γ) mRNA levels were detected by qRT-PCR.
32114163	7	65	theme	interferon	849:858	arg1	gamma					860:864	interferon gamma	849:864	interferon gamma (IFN-γ)	849:872	Interleukin-17A (IL-17A) and interferon gamma (IFN-γ) mRNA levels were detected by qRT-PCR.
32114163	7	65	theme	interferon	849:858	arg1	IFN-γ					867:871	IFN-γ	867:871	IFN-γ	867:871	Interleukin-17A (IL-17A) and interferon gamma (IFN-γ) mRNA levels were detected by qRT-PCR.
32114163	9	66	theme	including	1037:1045	arg1	occludin					1047:1054	intestinal tight junction protein including occludin	1003:1054	intestinal tight junction protein including occludin	1003:1054	Furthermore, intestinal tight junction protein including occludin, and serum lipopolysaccharide (LPS) level were also detected.
32114163	0	67	theme	intestinal	61:70	arg1	composition					83:93	intestinal microbiota composition	61:93	intestinal microbiota composition	61:93	Ping weisan alleviates chronic colitis in mice by regulating intestinal microbiota composition.
32114163	9	68	theme	tight	1014:1018	arg1	occludin					1047:1054	intestinal tight junction protein including occludin	1003:1054	intestinal tight junction protein including occludin	1003:1054	Furthermore, intestinal tight junction protein including occludin, and serum lipopolysaccharide (LPS) level were also detected.
32114163	2	69	from	colitis	404:410	arg1	mice					420:423	mice	420:423	mice	420:423	We have previously confirmed that PWS has the effect of alleviating DSS-induced chronic ulcerative colitis (UC) in mice.
32114163	4	70	theme	%	628:628	arg1	DSS					630:632	2.5% DSS	625:632	2.5% DSS in drinking water	625:650	MATERIALS AND METHODS Chronic colitis was induced in C57BL/6 mice with 2.5% DSS in drinking water.
32114163	15	71	theme	intestinal	1754:1763	arg1	bacteria					1765:1772	rebalancing intestinal bacteria	1742:1772	rebalancing intestinal bacteria	1742:1772	CONCLUSIONS Combining with our previous results, we found that PWS could exert anti-UC role by rebalancing intestinal bacteria.
32114163	14	72	theme	chronic	1615:1621	arg1	mice					1626:1629	chronic UC mice	1615:1629	chronic UC mice induced by DSS	1615:1644	PWS treatment significantly decreased the abundance of Bacteroidetes, but increased the abundance of Firmicutes in chronic UC mice induced by DSS.
32114163	15	73	theme	previous	1678:1685	arg1	results					1687:1693	our previous results	1674:1693	our previous results	1674:1693	CONCLUSIONS Combining with our previous results, we found that PWS could exert anti-UC role by rebalancing intestinal bacteria.
32114163	10	74	theme	RESULTS	1118:1124	arg1	PWS					1126:1128	RESULTS PWS	1118:1128	RESULTS PWS	1118:1128	RESULTS PWS relieved DSS-induced loss of body weight, and improved stool consistency and MPO activity in mice.
33480676	0	0	theme	ethylene	98:105	arg1	glycol					107:112	ethylene glycol	98:112	ethylene glycol	98:112	Potentiometric Determination of Circulating Glycoproteins by Boronic Acid End-Functionalized Poly(ethylene glycol)-Modified Electrode.
33480676	3	1	theme	cycloaddition	826:838	arg1	reaction					840:847	a copper-catalyzed azide-alkyne cycloaddition reaction	794:847	a copper-catalyzed azide-alkyne cycloaddition reaction	794:847	A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups was "grafted-to" a gold electrode modified with 11-azide-undecathiol by a copper-catalyzed azide-alkyne cycloaddition reaction.
33480676	2	2	theme	fetuin	489:494	arg1	determination					472:484	a label-free, no enzymatic, potentiometric determination	429:484	a label-free, no enzymatic, potentiometric determination of fetuin, a blood-circulating glycoprotein implicated in physiological and various pathological states	429:588	Taking advantage of boronic acid (BA) based SA-recognition chemistry, we here demonstrate a label-free, no enzymatic, potentiometric determination of fetuin, a blood-circulating glycoprotein implicated in physiological and various pathological states.
33480676	2	3	theme	acid	367:370	arg1	chemistry					398:406	boronic acid (BA) based SA-recognition chemistry	359:406	boronic acid (BA) based SA-recognition chemistry	359:406	Taking advantage of boronic acid (BA) based SA-recognition chemistry, we here demonstrate a label-free, no enzymatic, potentiometric determination of fetuin, a blood-circulating glycoprotein implicated in physiological and various pathological states.
33480676	5	4	with	technology	1091:1100	arg1	ease					1107:1110	ease	1107:1110	ease of downsizing and mass production	1107:1144	Our finding may have implications for developing a remarkably economic hemodiagnostic technology with ease of downsizing and mass production.
33480676	3	5	theme	pendent	700:706	arg1	groups					715:720	pendent alkyne groups	700:720	pendent alkyne groups	700:720	A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups was "grafted-to" a gold electrode modified with 11-azide-undecathiol by a copper-catalyzed azide-alkyne cycloaddition reaction.
33480676	4	6	theme	blood-collected	979:993	arg1	specimen					995:1002	blood-collected specimen	979:1002	blood-collected specimen	979:1002	Using the electrode, fetuin was potentiometrically detectable with a μM-order-sensitivity that is comparable to what is found in blood-collected specimen.
33480676	3	7	theme	grafted-to	727:736	arg1	acid					607:610	A phenylboronic acid	591:610	A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups	591:720	A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups was "grafted-to" a gold electrode modified with 11-azide-undecathiol by a copper-catalyzed azide-alkyne cycloaddition reaction.
33480676	3	7	theme	grafted-to	727:736	arg1	"					737:737	"grafted-to"	726:737	"grafted-to"	726:737	A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups was "grafted-to" a gold electrode modified with 11-azide-undecathiol by a copper-catalyzed azide-alkyne cycloaddition reaction.
33480676	0	8	dep	-Modified	114:122	arg1	glycol					107:112	ethylene glycol	98:112	ethylene glycol	98:112	Potentiometric Determination of Circulating Glycoproteins by Boronic Acid End-Functionalized Poly(ethylene glycol)-Modified Electrode.
33480676	2	9	gly	glycoprotein	517:528	arg1	fetuin					489:494	fetuin	489:494	fetuin	489:494	Taking advantage of boronic acid (BA) based SA-recognition chemistry, we here demonstrate a label-free, no enzymatic, potentiometric determination of fetuin, a blood-circulating glycoprotein implicated in physiological and various pathological states.
33480676	2	9	gly	glycoprotein	517:528	arg1	glycoprotein					517:528	a blood-circulating glycoprotein	497:528	a blood-circulating glycoprotein implicated in physiological and various pathological states	497:588	Taking advantage of boronic acid (BA) based SA-recognition chemistry, we here demonstrate a label-free, no enzymatic, potentiometric determination of fetuin, a blood-circulating glycoprotein implicated in physiological and various pathological states.
33480676	5	10	contain	have	1021:1024	arg2	implications					1026:1037	implications	1026:1037	implications for developing a remarkably economic hemodiagnostic technology with ease of downsizing and mass production	1026:1144	Our finding may have implications for developing a remarkably economic hemodiagnostic technology with ease of downsizing and mass production.
33480676	5	10	contain	have	1021:1024	arg1	finding					1009:1015	Our finding	1005:1015	Our finding	1005:1015	Our finding may have implications for developing a remarkably economic hemodiagnostic technology with ease of downsizing and mass production.
33480676	1	11	theme	sialic	186:191	arg1	SA					199:200	SA	199:200	SA	199:200	Despite tremendous complexity in glycan structure, sialic acid (SA) provides an analytically accessible index for glycosylation, owing to its uniquely anionic nature and glycan-chain terminal occupation.
33480676	1	11	theme	sialic	186:191	arg1	acid					193:196	sialic acid	186:196	sialic acid (SA)	186:201	Despite tremendous complexity in glycan structure, sialic acid (SA) provides an analytically accessible index for glycosylation, owing to its uniquely anionic nature and glycan-chain terminal occupation.
33480676	0	12	theme	-Modified	114:122	arg1	Electrode					124:132	Poly(ethylene glycol)-Modified Electrode	93:132	Poly(ethylene glycol)-Modified Electrode	93:132	Potentiometric Determination of Circulating Glycoproteins by Boronic Acid End-Functionalized Poly(ethylene glycol)-Modified Electrode.
33480676	2	13	dep	label-free	431:440	arg1	potentiometric					457:470	potentiometric	457:470	potentiometric	457:470	Taking advantage of boronic acid (BA) based SA-recognition chemistry, we here demonstrate a label-free, no enzymatic, potentiometric determination of fetuin, a blood-circulating glycoprotein implicated in physiological and various pathological states.
33480676	2	13	dep	label-free	431:440	arg1	enzymatic					446:454	enzymatic	446:454	enzymatic	446:454	Taking advantage of boronic acid (BA) based SA-recognition chemistry, we here demonstrate a label-free, no enzymatic, potentiometric determination of fetuin, a blood-circulating glycoprotein implicated in physiological and various pathological states.
33480676	4	14	located	found	970:974	arg1	specimen					995:1002	blood-collected specimen	979:1002	blood-collected specimen	979:1002	Using the electrode, fetuin was potentiometrically detectable with a μM-order-sensitivity that is comparable to what is found in blood-collected specimen.
33480676	4	14	located	found	970:974	arg2	what					962:965	what	962:965	what	962:965	Using the electrode, fetuin was potentiometrically detectable with a μM-order-sensitivity that is comparable to what is found in blood-collected specimen.
33480676	2	15	theme	chemistry	398:406	arg1	advantage					346:354	advantage	346:354	advantage of boronic acid (BA) based SA-recognition chemistry	346:406	Taking advantage of boronic acid (BA) based SA-recognition chemistry, we here demonstrate a label-free, no enzymatic, potentiometric determination of fetuin, a blood-circulating glycoprotein implicated in physiological and various pathological states.
33480676	1	16	from	complexity	154:163	arg1	structure					175:183	glycan structure	168:183	glycan structure	168:183	Despite tremendous complexity in glycan structure, sialic acid (SA) provides an analytically accessible index for glycosylation, owing to its uniquely anionic nature and glycan-chain terminal occupation.
33480676	3	17	theme	phenylboronic	593:605	arg1	"					737:737	"grafted-to"	726:737	"grafted-to"	726:737	A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups was "grafted-to" a gold electrode modified with 11-azide-undecathiol by a copper-catalyzed azide-alkyne cycloaddition reaction.
33480676	3	17	theme	phenylboronic	593:605	arg1	PBA					613:615	PBA	613:615	PBA	613:615	A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups was "grafted-to" a gold electrode modified with 11-azide-undecathiol by a copper-catalyzed azide-alkyne cycloaddition reaction.
33480676	3	17	theme	phenylboronic	593:605	arg1	acid					607:610	A phenylboronic acid	591:610	A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups	591:720	A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups was "grafted-to" a gold electrode modified with 11-azide-undecathiol by a copper-catalyzed azide-alkyne cycloaddition reaction.
33480676	2	18	theme	SA-recognition	383:396	arg1	chemistry					398:406	boronic acid (BA) based SA-recognition chemistry	359:406	boronic acid (BA) based SA-recognition chemistry	359:406	Taking advantage of boronic acid (BA) based SA-recognition chemistry, we here demonstrate a label-free, no enzymatic, potentiometric determination of fetuin, a blood-circulating glycoprotein implicated in physiological and various pathological states.
33480676	0	19	theme	Potentiometric	0:13	arg1	Determination					15:27	Potentiometric Determination	0:27	Potentiometric Determination of Circulating Glycoproteins by Boronic Acid	0:72	Potentiometric Determination of Circulating Glycoproteins by Boronic Acid End-Functionalized Poly(ethylene glycol)-Modified Electrode.
33480676	2	20	theme	based	377:381	arg1	chemistry					398:406	boronic acid (BA) based SA-recognition chemistry	359:406	boronic acid (BA) based SA-recognition chemistry	359:406	Taking advantage of boronic acid (BA) based SA-recognition chemistry, we here demonstrate a label-free, no enzymatic, potentiometric determination of fetuin, a blood-circulating glycoprotein implicated in physiological and various pathological states.
33480676	0	21	theme	Circulating	32:42	arg1	Glycoproteins					44:56	Circulating Glycoproteins	32:56	Circulating Glycoproteins	32:56	Potentiometric Determination of Circulating Glycoproteins by Boronic Acid End-Functionalized Poly(ethylene glycol)-Modified Electrode.
33480676	1	22	theme	anionic	286:292	arg1	nature					294:299	its uniquely anionic nature	273:299	its uniquely anionic nature	273:299	Despite tremendous complexity in glycan structure, sialic acid (SA) provides an analytically accessible index for glycosylation, owing to its uniquely anionic nature and glycan-chain terminal occupation.
33480676	2	23	theme	label-free	431:440	arg1	determination					472:484	a label-free, no enzymatic, potentiometric determination	429:484	a label-free, no enzymatic, potentiometric determination of fetuin, a blood-circulating glycoprotein implicated in physiological and various pathological states	429:588	Taking advantage of boronic acid (BA) based SA-recognition chemistry, we here demonstrate a label-free, no enzymatic, potentiometric determination of fetuin, a blood-circulating glycoprotein implicated in physiological and various pathological states.
33480676	2	24	theme	pathological	570:581	arg1	states					583:588	various pathological states	562:588	various pathological states	562:588	Taking advantage of boronic acid (BA) based SA-recognition chemistry, we here demonstrate a label-free, no enzymatic, potentiometric determination of fetuin, a blood-circulating glycoprotein implicated in physiological and various pathological states.
33480676	5	25	theme	mass	1130:1133	arg1	production					1135:1144	mass production	1130:1144	mass production	1130:1144	Our finding may have implications for developing a remarkably economic hemodiagnostic technology with ease of downsizing and mass production.
33480676	3	26	dep	acid	607:610	arg1	PEG					662:664	PEG	662:664	PEG	662:664	A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups was "grafted-to" a gold electrode modified with 11-azide-undecathiol by a copper-catalyzed azide-alkyne cycloaddition reaction.
33480676	3	26	dep	acid	607:610	arg1	poly					639:642	ω-end-functionalized poly	618:642	A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups	591:720	A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups was "grafted-to" a gold electrode modified with 11-azide-undecathiol by a copper-catalyzed azide-alkyne cycloaddition reaction.
33480676	3	26	dep	acid	607:610	arg1	glycol					653:658	ethylene glycol	644:658	ethylene glycol	644:658	A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups was "grafted-to" a gold electrode modified with 11-azide-undecathiol by a copper-catalyzed azide-alkyne cycloaddition reaction.
33480676	3	27	theme	gold	741:744	arg1	electrode					746:754	a gold electrode	739:754	a gold electrode modified with 11-azide-undecathiol by a copper-catalyzed azide-alkyne cycloaddition reaction	739:847	A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups was "grafted-to" a gold electrode modified with 11-azide-undecathiol by a copper-catalyzed azide-alkyne cycloaddition reaction.
33480676	2	28	theme	various	562:568	arg1	states					583:588	various pathological states	562:588	various pathological states	562:588	Taking advantage of boronic acid (BA) based SA-recognition chemistry, we here demonstrate a label-free, no enzymatic, potentiometric determination of fetuin, a blood-circulating glycoprotein implicated in physiological and various pathological states.
33480676	5	29	theme	production	1135:1144	arg1	ease					1107:1110	ease	1107:1110	ease of downsizing and mass production	1107:1144	Our finding may have implications for developing a remarkably economic hemodiagnostic technology with ease of downsizing and mass production.
33480676	5	30	theme	economic	1067:1074	arg1	technology					1091:1100	a remarkably economic hemodiagnostic technology	1054:1100	a remarkably economic hemodiagnostic technology with ease of downsizing and mass production	1054:1144	Our finding may have implications for developing a remarkably economic hemodiagnostic technology with ease of downsizing and mass production.
33480676	0	31	theme	Glycoproteins	44:56	arg1	Determination					15:27	Potentiometric Determination	0:27	Potentiometric Determination of Circulating Glycoproteins by Boronic Acid	0:72	Potentiometric Determination of Circulating Glycoproteins by Boronic Acid End-Functionalized Poly(ethylene glycol)-Modified Electrode.
33480676	1	32	theme	glycan-chain	305:316	arg1	occupation					327:336	glycan-chain terminal occupation	305:336	glycan-chain terminal occupation	305:336	Despite tremendous complexity in glycan structure, sialic acid (SA) provides an analytically accessible index for glycosylation, owing to its uniquely anionic nature and glycan-chain terminal occupation.
33480676	3	33	theme	ω-end-functionalized	618:637	arg1	PEG					662:664	PEG	662:664	PEG	662:664	A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups was "grafted-to" a gold electrode modified with 11-azide-undecathiol by a copper-catalyzed azide-alkyne cycloaddition reaction.
33480676	3	33	theme	ω-end-functionalized	618:637	arg1	poly					639:642	ω-end-functionalized poly	618:642	A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups	591:720	A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups was "grafted-to" a gold electrode modified with 11-azide-undecathiol by a copper-catalyzed azide-alkyne cycloaddition reaction.
33480676	3	33	theme	ω-end-functionalized	618:637	arg1	glycol					653:658	ethylene glycol	644:658	ethylene glycol	644:658	A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups was "grafted-to" a gold electrode modified with 11-azide-undecathiol by a copper-catalyzed azide-alkyne cycloaddition reaction.
33480676	1	34	theme	terminal	318:325	arg1	occupation					327:336	glycan-chain terminal occupation	305:336	glycan-chain terminal occupation	305:336	Despite tremendous complexity in glycan structure, sialic acid (SA) provides an analytically accessible index for glycosylation, owing to its uniquely anionic nature and glycan-chain terminal occupation.
33480676	2	35	theme	boronic	359:365	arg1	BA					373:374	BA	373:374	BA	373:374	Taking advantage of boronic acid (BA) based SA-recognition chemistry, we here demonstrate a label-free, no enzymatic, potentiometric determination of fetuin, a blood-circulating glycoprotein implicated in physiological and various pathological states.
33480676	2	35	theme	boronic	359:365	arg1	acid					367:370	boronic acid	359:370	boronic acid (BA) based SA-recognition chemistry	359:406	Taking advantage of boronic acid (BA) based SA-recognition chemistry, we here demonstrate a label-free, no enzymatic, potentiometric determination of fetuin, a blood-circulating glycoprotein implicated in physiological and various pathological states.
33480676	0	36	theme	Boronic	61:67	arg1	Acid					69:72	Boronic Acid	61:72	Boronic Acid	61:72	Potentiometric Determination of Circulating Glycoproteins by Boronic Acid End-Functionalized Poly(ethylene glycol)-Modified Electrode.
33480676	3	37	with	poly	639:642	arg1	unit					687:690	an α-tethering unit	672:690	an α-tethering unit bearing pendent alkyne groups	672:720	A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups was "grafted-to" a gold electrode modified with 11-azide-undecathiol by a copper-catalyzed azide-alkyne cycloaddition reaction.
33480676	3	38	theme	α-tethering	675:685	arg1	unit					687:690	an α-tethering unit	672:690	an α-tethering unit bearing pendent alkyne groups	672:720	A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups was "grafted-to" a gold electrode modified with 11-azide-undecathiol by a copper-catalyzed azide-alkyne cycloaddition reaction.
33480676	3	39	dep	"	737:737	arg1	electrode					746:754	a gold electrode	739:754	a gold electrode modified with 11-azide-undecathiol by a copper-catalyzed azide-alkyne cycloaddition reaction	739:847	A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups was "grafted-to" a gold electrode modified with 11-azide-undecathiol by a copper-catalyzed azide-alkyne cycloaddition reaction.
33480676	3	40	theme	ethylene	644:651	arg1	poly					639:642	ω-end-functionalized poly	618:642	A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups	591:720	A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups was "grafted-to" a gold electrode modified with 11-azide-undecathiol by a copper-catalyzed azide-alkyne cycloaddition reaction.
33480676	3	40	theme	ethylene	644:651	arg1	glycol					653:658	ethylene glycol	644:658	ethylene glycol	644:658	A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups was "grafted-to" a gold electrode modified with 11-azide-undecathiol by a copper-catalyzed azide-alkyne cycloaddition reaction.
33480676	5	41	theme	hemodiagnostic	1076:1089	arg1	technology					1091:1100	a remarkably economic hemodiagnostic technology	1054:1100	a remarkably economic hemodiagnostic technology with ease of downsizing and mass production	1054:1144	Our finding may have implications for developing a remarkably economic hemodiagnostic technology with ease of downsizing and mass production.
33480676	1	42	theme	tremendous	143:152	arg1	complexity					154:163	tremendous complexity	143:163	tremendous complexity in glycan structure	143:183	Despite tremendous complexity in glycan structure, sialic acid (SA) provides an analytically accessible index for glycosylation, owing to its uniquely anionic nature and glycan-chain terminal occupation.
33480676	1	43	theme	accessible	228:237	arg1	index					239:243	an analytically accessible index	212:243	an analytically accessible index for glycosylation	212:261	Despite tremendous complexity in glycan structure, sialic acid (SA) provides an analytically accessible index for glycosylation, owing to its uniquely anionic nature and glycan-chain terminal occupation.
33480676	5	44	theme	downsizing	1115:1124	arg1	ease					1107:1110	ease	1107:1110	ease of downsizing and mass production	1107:1144	Our finding may have implications for developing a remarkably economic hemodiagnostic technology with ease of downsizing and mass production.
33480676	2	45	theme	blood-circulating	499:515	arg1	fetuin					489:494	fetuin	489:494	fetuin	489:494	Taking advantage of boronic acid (BA) based SA-recognition chemistry, we here demonstrate a label-free, no enzymatic, potentiometric determination of fetuin, a blood-circulating glycoprotein implicated in physiological and various pathological states.
33480676	2	45	theme	blood-circulating	499:515	arg1	glycoprotein					517:528	a blood-circulating glycoprotein	497:528	a blood-circulating glycoprotein implicated in physiological and various pathological states	497:588	Taking advantage of boronic acid (BA) based SA-recognition chemistry, we here demonstrate a label-free, no enzymatic, potentiometric determination of fetuin, a blood-circulating glycoprotein implicated in physiological and various pathological states.
33480676	4	46	with	detectable	901:910	arg1	comparable					948:957	comparable	948:957	comparable	948:957	Using the electrode, fetuin was potentiometrically detectable with a μM-order-sensitivity that is comparable to what is found in blood-collected specimen.
33480676	4	46	with	detectable	901:910	arg1	μM-order-sensitivity					919:938	a μM-order-sensitivity	917:938	a μM-order-sensitivity that is comparable to what is found in blood-collected specimen	917:1002	Using the electrode, fetuin was potentiometrically detectable with a μM-order-sensitivity that is comparable to what is found in blood-collected specimen.
33480676	3	47	theme	copper-catalyzed	796:811	arg1	reaction					840:847	a copper-catalyzed azide-alkyne cycloaddition reaction	794:847	a copper-catalyzed azide-alkyne cycloaddition reaction	794:847	A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups was "grafted-to" a gold electrode modified with 11-azide-undecathiol by a copper-catalyzed azide-alkyne cycloaddition reaction.
33480676	3	48	theme	alkyne	708:713	arg1	groups					715:720	pendent alkyne groups	700:720	pendent alkyne groups	700:720	A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups was "grafted-to" a gold electrode modified with 11-azide-undecathiol by a copper-catalyzed azide-alkyne cycloaddition reaction.
33480676	1	49	theme	glycan	168:173	arg1	structure					175:183	glycan structure	168:183	glycan structure	168:183	Despite tremendous complexity in glycan structure, sialic acid (SA) provides an analytically accessible index for glycosylation, owing to its uniquely anionic nature and glycan-chain terminal occupation.
33480676	3	50	theme	azide-alkyne	813:824	arg1	reaction					840:847	a copper-catalyzed azide-alkyne cycloaddition reaction	794:847	a copper-catalyzed azide-alkyne cycloaddition reaction	794:847	A phenylboronic acid (PBA) ω-end-functionalized poly(ethylene glycol) (PEG) with an α-tethering unit bearing pendent alkyne groups was "grafted-to" a gold electrode modified with 11-azide-undecathiol by a copper-catalyzed azide-alkyne cycloaddition reaction.
32488784	0	0	theme	Hydrazide	100:108	arg1	Effect					14:19	Antifibrotics Effect	0:19	Antifibrotics Effect of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide in Mice with BCG-Induced Granulomatosis	0:148	Antifibrotics Effect of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide in Mice with BCG-Induced Granulomatosis Depends on Administration Route.
32488784	2	1	theme	LEDZ	484:487	arg1	inhalation					489:498	LEDZ inhalation	484:498	LEDZ inhalation	484:498	LEDZ inhalation proved to be more effective in reducing fibrosis severity, both in the lungs and liver.
32488784	4	2	theme	target	856:861	arg1	organs					863:868	the target organs	852:868	the target organs	852:868	This suggest that drug administration routes and delivery to the target organs are crucially important in the therapy of tuberculosis.
32488784	0	3	theme	Acid	95:98	arg1	Hydrazide					100:108	Isonicotinic Acid Hydrazide	82:108	Isonicotinic Acid Hydrazide in Mice with BCG-Induced Granulomatosis	82:148	Antifibrotics Effect of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide in Mice with BCG-Induced Granulomatosis Depends on Administration Route.
32488784	0	4	from	Effect	14:19	arg1	Mice					113:116	Mice	113:116	Mice with BCG-Induced Granulomatosis	113:148	Antifibrotics Effect of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide in Mice with BCG-Induced Granulomatosis Depends on Administration Route.
32488784	4	5	from	important	884:892	arg1	therapy					901:907	the therapy	897:907	the therapy of tuberculosis	897:923	This suggest that drug administration routes and delivery to the target organs are crucially important in the therapy of tuberculosis.
32488784	4	6	from	therapy	901:907	arg1	important					884:892	important	884:892	important	884:892	This suggest that drug administration routes and delivery to the target organs are crucially important in the therapy of tuberculosis.
32488784	3	7	theme	antifibrotic	619:630	arg1	effect					632:637	the antifibrotic effect	615:637	the antifibrotic effect	615:637	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	2	8	dep	reducing	531:538	arg1	both					559:562	both	559:562	both	559:562	LEDZ inhalation proved to be more effective in reducing fibrosis severity, both in the lungs and liver.
32488784	1	9	theme	dextrazide	385:394	arg1	inhalation					314:323	inhalation	314:323	inhalation	314:323	We studied the response of the extracellular matrix of the lungs and liver in mice with BCGinduced granulomatosis (3 months) after inhalation and intraperitoneal administration of liposome-encapsulated dextrazide (LEDZ): a conjugate of oxidized dextran (40 kDa) and isonicotinic acid hydrazide (INH).
32488784	1	9	theme	dextrazide	385:394	arg1	administration					345:358	intraperitoneal administration	329:358	intraperitoneal administration of liposome-encapsulated dextrazide (LEDZ): a conjugate of oxidized dextran (40 kDa) and isonicotinic acid hydrazide (INH)	329:481	We studied the response of the extracellular matrix of the lungs and liver in mice with BCGinduced granulomatosis (3 months) after inhalation and intraperitoneal administration of liposome-encapsulated dextrazide (LEDZ): a conjugate of oxidized dextran (40 kDa) and isonicotinic acid hydrazide (INH).
32488784	3	10	from	degradation	765:775	arg1	liver					784:788	the liver	780:788	the liver	780:788	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	10	from	degradation	765:775	arg1	lungs					715:719	the lungs	711:719	the lungs	711:719	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	1	11	theme	BCGinduced	271:280	arg1	granulomatosis					282:295	BCGinduced granulomatosis	271:295	BCGinduced granulomatosis	271:295	We studied the response of the extracellular matrix of the lungs and liver in mice with BCGinduced granulomatosis (3 months) after inhalation and intraperitoneal administration of liposome-encapsulated dextrazide (LEDZ): a conjugate of oxidized dextran (40 kDa) and isonicotinic acid hydrazide (INH).
32488784	0	12	from	Dextran	70:76	arg1	Mice					113:116	Mice	113:116	Mice with BCG-Induced Granulomatosis	113:148	Antifibrotics Effect of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide in Mice with BCG-Induced Granulomatosis Depends on Administration Route.
32488784	1	13	theme	isonicotinic	449:460	arg1	INH					478:480	INH	478:480	INH	478:480	We studied the response of the extracellular matrix of the lungs and liver in mice with BCGinduced granulomatosis (3 months) after inhalation and intraperitoneal administration of liposome-encapsulated dextrazide (LEDZ): a conjugate of oxidized dextran (40 kDa) and isonicotinic acid hydrazide (INH).
32488784	1	13	theme	isonicotinic	449:460	arg1	hydrazide					467:475	isonicotinic acid hydrazide	449:475	isonicotinic acid hydrazide (INH)	449:481	We studied the response of the extracellular matrix of the lungs and liver in mice with BCGinduced granulomatosis (3 months) after inhalation and intraperitoneal administration of liposome-encapsulated dextrazide (LEDZ): a conjugate of oxidized dextran (40 kDa) and isonicotinic acid hydrazide (INH).
32488784	5	14	theme	LEDZ	962:965	arg1	route					982:986	LEDZ administration route	962:986	LEDZ administration route	962:986	The antifibrotic effect depended on LEDZ administration route and was more potent after LEDZ inhalation.
32488784	0	15	theme	BCG-Induced	123:133	arg1	Granulomatosis					135:148	BCG-Induced Granulomatosis	123:148	BCG-Induced Granulomatosis	123:148	Antifibrotics Effect of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide in Mice with BCG-Induced Granulomatosis Depends on Administration Route.
32488784	1	16	theme	acid	462:465	arg1	INH					478:480	INH	478:480	INH	478:480	We studied the response of the extracellular matrix of the lungs and liver in mice with BCGinduced granulomatosis (3 months) after inhalation and intraperitoneal administration of liposome-encapsulated dextrazide (LEDZ): a conjugate of oxidized dextran (40 kDa) and isonicotinic acid hydrazide (INH).
32488784	1	16	theme	acid	462:465	arg1	hydrazide					467:475	isonicotinic acid hydrazide	449:475	isonicotinic acid hydrazide (INH)	449:481	We studied the response of the extracellular matrix of the lungs and liver in mice with BCGinduced granulomatosis (3 months) after inhalation and intraperitoneal administration of liposome-encapsulated dextrazide (LEDZ): a conjugate of oxidized dextran (40 kDa) and isonicotinic acid hydrazide (INH).
32488784	5	17	theme	administration	967:980	arg1	route					982:986	LEDZ administration route	962:986	LEDZ administration route	962:986	The antifibrotic effect depended on LEDZ administration route and was more potent after LEDZ inhalation.
32488784	4	18	theme	tuberculosis	912:923	arg1	therapy					901:907	the therapy	897:907	the therapy of tuberculosis	897:923	This suggest that drug administration routes and delivery to the target organs are crucially important in the therapy of tuberculosis.
32488784	1	19	theme	hydrazide	467:475	arg1	conjugate					406:414	a conjugate	404:414	liposome-encapsulated dextrazide (LEDZ): a conjugate of oxidized dextran (40 kDa) and isonicotinic acid hydrazide (INH)	363:481	We studied the response of the extracellular matrix of the lungs and liver in mice with BCGinduced granulomatosis (3 months) after inhalation and intraperitoneal administration of liposome-encapsulated dextrazide (LEDZ): a conjugate of oxidized dextran (40 kDa) and isonicotinic acid hydrazide (INH).
32488784	3	20	from	synthesis	698:706	arg1	liver					784:788	the liver	780:788	the liver	780:788	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	20	from	synthesis	698:706	arg1	lungs					715:719	the lungs	711:719	the lungs	711:719	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	0	21	theme	Antifibrotics	0:12	arg1	Effect					14:19	Antifibrotics Effect	0:19	Antifibrotics Effect of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide in Mice with BCG-Induced Granulomatosis	0:148	Antifibrotics Effect of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide in Mice with BCG-Induced Granulomatosis Depends on Administration Route.
32488784	3	22	theme	collagen	733:740	arg1	different					644:652	different	644:652	different	644:652	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	22	theme	collagen	733:740	arg1	degradation					665:675	increased degradation	655:675	increased degradation	655:675	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	22	theme	collagen	733:740	arg1	synthesis					742:750	reduced collagen synthesis	725:750	reduced collagen synthesis	725:750	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	22	theme	collagen	733:740	arg1	synthesis					698:706	reduced collagen synthesis	681:706	reduced collagen synthesis	681:706	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	22	theme	collagen	733:740	arg1	mechanisms					601:610	the mechanisms	597:610	the mechanisms of the antifibrotic effect	597:637	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	23	from	synthesis	742:750	arg1	liver					784:788	the liver	780:788	the liver	780:788	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	23	from	synthesis	742:750	arg1	lungs					715:719	the lungs	711:719	the lungs	711:719	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	0	24	with	Mice	113:116	arg1	Granulomatosis					135:148	BCG-Induced Granulomatosis	123:148	BCG-Induced Granulomatosis	123:148	Antifibrotics Effect of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide in Mice with BCG-Induced Granulomatosis Depends on Administration Route.
32488784	1	25	dep	studied	186:192	arg1	months					300:305	3 months	298:305	3 months	298:305	We studied the response of the extracellular matrix of the lungs and liver in mice with BCGinduced granulomatosis (3 months) after inhalation and intraperitoneal administration of liposome-encapsulated dextrazide (LEDZ): a conjugate of oxidized dextran (40 kDa) and isonicotinic acid hydrazide (INH).
32488784	0	26	theme	Liposome-Encapsulated	24:44	arg1	Composition					46:56	Liposome-Encapsulated Composition	24:56	Liposome-Encapsulated Composition	24:56	Antifibrotics Effect of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide in Mice with BCG-Induced Granulomatosis Depends on Administration Route.
32488784	1	27	dep	dextrazide	385:394	arg1	conjugate					406:414	a conjugate	404:414	liposome-encapsulated dextrazide (LEDZ): a conjugate of oxidized dextran (40 kDa) and isonicotinic acid hydrazide (INH)	363:481	We studied the response of the extracellular matrix of the lungs and liver in mice with BCGinduced granulomatosis (3 months) after inhalation and intraperitoneal administration of liposome-encapsulated dextrazide (LEDZ): a conjugate of oxidized dextran (40 kDa) and isonicotinic acid hydrazide (INH).
32488784	1	28	theme	extracellular	214:226	arg1	matrix					228:233	the extracellular matrix	210:233	the extracellular matrix of the lungs and liver	210:256	We studied the response of the extracellular matrix of the lungs and liver in mice with BCGinduced granulomatosis (3 months) after inhalation and intraperitoneal administration of liposome-encapsulated dextrazide (LEDZ): a conjugate of oxidized dextran (40 kDa) and isonicotinic acid hydrazide (INH).
32488784	5	29	theme	antifibrotic	930:941	arg1	effect					943:948	The antifibrotic effect	926:948	The antifibrotic effect	926:948	The antifibrotic effect depended on LEDZ administration route and was more potent after LEDZ inhalation.
32488784	5	29	theme	antifibrotic	930:941	arg1	potent					1001:1006	potent	1001:1006	potent	1001:1006	The antifibrotic effect depended on LEDZ administration route and was more potent after LEDZ inhalation.
32488784	3	30	dep	different	644:652	arg1	different					644:652	different	644:652	different	644:652	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	30	dep	different	644:652	arg1	degradation					665:675	increased degradation	655:675	increased degradation	655:675	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	30	dep	different	644:652	arg1	synthesis					742:750	reduced collagen synthesis	725:750	reduced collagen synthesis	725:750	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	30	dep	different	644:652	arg1	synthesis					698:706	reduced collagen synthesis	681:706	reduced collagen synthesis	681:706	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	30	dep	different	644:652	arg1	mechanisms					601:610	the mechanisms	597:610	the mechanisms of the antifibrotic effect	597:637	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	2	31	theme	fibrosis	540:547	arg1	severity					549:556	fibrosis severity	540:556	fibrosis severity	540:556	LEDZ inhalation proved to be more effective in reducing fibrosis severity, both in the lungs and liver.
32488784	0	32	from	Hydrazide	100:108	arg1	Mice					113:116	Mice	113:116	Mice with BCG-Induced Granulomatosis	113:148	Antifibrotics Effect of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide in Mice with BCG-Induced Granulomatosis Depends on Administration Route.
32488784	1	33	theme	matrix	228:233	arg1	response					198:205	the response	194:205	the response of the extracellular matrix of the lungs and liver	194:256	We studied the response of the extracellular matrix of the lungs and liver in mice with BCGinduced granulomatosis (3 months) after inhalation and intraperitoneal administration of liposome-encapsulated dextrazide (LEDZ): a conjugate of oxidized dextran (40 kDa) and isonicotinic acid hydrazide (INH).
32488784	3	34	from	degradation	665:675	arg1	liver					784:788	the liver	780:788	the liver	780:788	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	34	from	degradation	665:675	arg1	lungs					715:719	the lungs	711:719	the lungs	711:719	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	35	theme	reduced	725:731	arg1	different					644:652	different	644:652	different	644:652	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	35	theme	reduced	725:731	arg1	degradation					665:675	increased degradation	655:675	increased degradation	655:675	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	35	theme	reduced	725:731	arg1	synthesis					742:750	reduced collagen synthesis	725:750	reduced collagen synthesis	725:750	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	35	theme	reduced	725:731	arg1	synthesis					698:706	reduced collagen synthesis	681:706	reduced collagen synthesis	681:706	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	35	theme	reduced	725:731	arg1	mechanisms					601:610	the mechanisms	597:610	the mechanisms of the antifibrotic effect	597:637	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	0	36	theme	Administration	161:174	arg1	Route					176:180	Administration Route	161:180	Administration Route	161:180	Antifibrotics Effect of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide in Mice with BCG-Induced Granulomatosis Depends on Administration Route.
32488784	3	37	theme	effect	632:637	arg1	different					644:652	different	644:652	different	644:652	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	37	theme	effect	632:637	arg1	degradation					665:675	increased degradation	655:675	increased degradation	655:675	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	37	theme	effect	632:637	arg1	synthesis					742:750	reduced collagen synthesis	725:750	reduced collagen synthesis	725:750	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	37	theme	effect	632:637	arg1	synthesis					698:706	reduced collagen synthesis	681:706	reduced collagen synthesis	681:706	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	37	theme	effect	632:637	arg1	mechanisms					601:610	the mechanisms	597:610	the mechanisms of the antifibrotic effect	597:637	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	1	38	theme	liposome-encapsulated	363:383	arg1	LEDZ					397:400	LEDZ	397:400	LEDZ	397:400	We studied the response of the extracellular matrix of the lungs and liver in mice with BCGinduced granulomatosis (3 months) after inhalation and intraperitoneal administration of liposome-encapsulated dextrazide (LEDZ): a conjugate of oxidized dextran (40 kDa) and isonicotinic acid hydrazide (INH).
32488784	1	38	theme	liposome-encapsulated	363:383	arg1	dextrazide					385:394	liposome-encapsulated dextrazide	363:394	liposome-encapsulated dextrazide (LEDZ): a conjugate of oxidized dextran (40 kDa) and isonicotinic acid hydrazide (INH)	363:481	We studied the response of the extracellular matrix of the lungs and liver in mice with BCGinduced granulomatosis (3 months) after inhalation and intraperitoneal administration of liposome-encapsulated dextrazide (LEDZ): a conjugate of oxidized dextran (40 kDa) and isonicotinic acid hydrazide (INH).
32488784	0	39	theme	Composition	46:56	arg1	Effect					14:19	Antifibrotics Effect	0:19	Antifibrotics Effect of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide in Mice with BCG-Induced Granulomatosis	0:148	Antifibrotics Effect of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide in Mice with BCG-Induced Granulomatosis Depends on Administration Route.
32488784	2	40	dep	lungs	571:575	arg1	the					567:569	the	567:569	the	567:569	LEDZ inhalation proved to be more effective in reducing fibrosis severity, both in the lungs and liver.
32488784	5	41	theme	LEDZ	1014:1017	arg1	inhalation					1019:1028	LEDZ inhalation	1014:1028	LEDZ inhalation	1014:1028	The antifibrotic effect depended on LEDZ administration route and was more potent after LEDZ inhalation.
32488784	3	42	theme	collagen	756:763	arg1	degradation					765:775	collagen degradation	756:775	collagen degradation	756:775	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	0	43	theme	Dextran	70:76	arg1	Effect					14:19	Antifibrotics Effect	0:19	Antifibrotics Effect of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide in Mice with BCG-Induced Granulomatosis	0:148	Antifibrotics Effect of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide in Mice with BCG-Induced Granulomatosis Depends on Administration Route.
32488784	1	44	theme	oxidized	419:426	arg1	dextran					428:434	oxidized dextran	419:434	oxidized dextran (40 kDa)	419:443	We studied the response of the extracellular matrix of the lungs and liver in mice with BCGinduced granulomatosis (3 months) after inhalation and intraperitoneal administration of liposome-encapsulated dextrazide (LEDZ): a conjugate of oxidized dextran (40 kDa) and isonicotinic acid hydrazide (INH).
32488784	1	44	theme	oxidized	419:426	arg1	kDa					440:442	40 kDa	437:442	40 kDa	437:442	We studied the response of the extracellular matrix of the lungs and liver in mice with BCGinduced granulomatosis (3 months) after inhalation and intraperitoneal administration of liposome-encapsulated dextrazide (LEDZ): a conjugate of oxidized dextran (40 kDa) and isonicotinic acid hydrazide (INH).
32488784	4	45	theme	administration	814:827	arg1	routes					829:834	drug administration routes	809:834	drug administration routes	809:834	This suggest that drug administration routes and delivery to the target organs are crucially important in the therapy of tuberculosis.
32488784	0	46	theme	Oxidized	61:68	arg1	Dextran					70:76	Oxidized Dextran	61:76	Oxidized Dextran	61:76	Antifibrotics Effect of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide in Mice with BCG-Induced Granulomatosis Depends on Administration Route.
32488784	1	47	theme	dextran	428:434	arg1	conjugate					406:414	a conjugate	404:414	liposome-encapsulated dextrazide (LEDZ): a conjugate of oxidized dextran (40 kDa) and isonicotinic acid hydrazide (INH)	363:481	We studied the response of the extracellular matrix of the lungs and liver in mice with BCGinduced granulomatosis (3 months) after inhalation and intraperitoneal administration of liposome-encapsulated dextrazide (LEDZ): a conjugate of oxidized dextran (40 kDa) and isonicotinic acid hydrazide (INH).
32488784	4	48	theme	drug	809:812	arg1	routes					829:834	drug administration routes	809:834	drug administration routes	809:834	This suggest that drug administration routes and delivery to the target organs are crucially important in the therapy of tuberculosis.
32488784	1	49	with	mice	261:264	arg1	granulomatosis					282:295	BCGinduced granulomatosis	271:295	BCGinduced granulomatosis	271:295	We studied the response of the extracellular matrix of the lungs and liver in mice with BCGinduced granulomatosis (3 months) after inhalation and intraperitoneal administration of liposome-encapsulated dextrazide (LEDZ): a conjugate of oxidized dextran (40 kDa) and isonicotinic acid hydrazide (INH).
32488784	1	50	theme	intraperitoneal	329:343	arg1	administration					345:358	intraperitoneal administration	329:358	intraperitoneal administration of liposome-encapsulated dextrazide (LEDZ): a conjugate of oxidized dextran (40 kDa) and isonicotinic acid hydrazide (INH)	329:481	We studied the response of the extracellular matrix of the lungs and liver in mice with BCGinduced granulomatosis (3 months) after inhalation and intraperitoneal administration of liposome-encapsulated dextrazide (LEDZ): a conjugate of oxidized dextran (40 kDa) and isonicotinic acid hydrazide (INH).
32488784	3	51	theme	increased	655:663	arg1	different					644:652	different	644:652	different	644:652	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	51	theme	increased	655:663	arg1	degradation					665:675	increased degradation	655:675	increased degradation	655:675	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	51	theme	increased	655:663	arg1	synthesis					742:750	reduced collagen synthesis	725:750	reduced collagen synthesis	725:750	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	51	theme	increased	655:663	arg1	synthesis					698:706	reduced collagen synthesis	681:706	reduced collagen synthesis	681:706	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	51	theme	increased	655:663	arg1	mechanisms					601:610	the mechanisms	597:610	the mechanisms of the antifibrotic effect	597:637	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	1	52	theme	lungs	242:246	arg1	matrix					228:233	the extracellular matrix	210:233	the extracellular matrix of the lungs and liver	210:256	We studied the response of the extracellular matrix of the lungs and liver in mice with BCGinduced granulomatosis (3 months) after inhalation and intraperitoneal administration of liposome-encapsulated dextrazide (LEDZ): a conjugate of oxidized dextran (40 kDa) and isonicotinic acid hydrazide (INH).
32488784	0	53	from	Mice	113:116	arg1	Effect					14:19	Antifibrotics Effect	0:19	Antifibrotics Effect of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide in Mice with BCG-Induced Granulomatosis	0:148	Antifibrotics Effect of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide in Mice with BCG-Induced Granulomatosis Depends on Administration Route.
32488784	0	54	theme	Isonicotinic	82:93	arg1	Hydrazide					100:108	Isonicotinic Acid Hydrazide	82:108	Isonicotinic Acid Hydrazide in Mice with BCG-Induced Granulomatosis	82:148	Antifibrotics Effect of Liposome-Encapsulated Composition of Oxidized Dextran and Isonicotinic Acid Hydrazide in Mice with BCG-Induced Granulomatosis Depends on Administration Route.
32488784	1	55	theme	liver	252:256	arg1	matrix					228:233	the extracellular matrix	210:233	the extracellular matrix of the lungs and liver	210:256	We studied the response of the extracellular matrix of the lungs and liver in mice with BCGinduced granulomatosis (3 months) after inhalation and intraperitoneal administration of liposome-encapsulated dextrazide (LEDZ): a conjugate of oxidized dextran (40 kDa) and isonicotinic acid hydrazide (INH).
32488784	3	56	theme	reduced	681:687	arg1	different					644:652	different	644:652	different	644:652	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	56	theme	reduced	681:687	arg1	degradation					665:675	increased degradation	655:675	increased degradation	655:675	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	56	theme	reduced	681:687	arg1	synthesis					742:750	reduced collagen synthesis	725:750	reduced collagen synthesis	725:750	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	56	theme	reduced	681:687	arg1	synthesis					698:706	reduced collagen synthesis	681:706	reduced collagen synthesis	681:706	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	56	theme	reduced	681:687	arg1	mechanisms					601:610	the mechanisms	597:610	the mechanisms of the antifibrotic effect	597:637	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	57	theme	collagen	689:696	arg1	different					644:652	different	644:652	different	644:652	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	57	theme	collagen	689:696	arg1	degradation					665:675	increased degradation	655:675	increased degradation	655:675	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	57	theme	collagen	689:696	arg1	synthesis					742:750	reduced collagen synthesis	725:750	reduced collagen synthesis	725:750	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	57	theme	collagen	689:696	arg1	synthesis					698:706	reduced collagen synthesis	681:706	reduced collagen synthesis	681:706	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32488784	3	57	theme	collagen	689:696	arg1	mechanisms					601:610	the mechanisms	597:610	the mechanisms of the antifibrotic effect	597:637	However, the mechanisms of the antifibrotic effect were different: increased degradation and reduced collagen synthesis in the lungs and reduced collagen synthesis and collagen degradation in the liver.
32125262	5	0	theme	58.2 mol	641:648	arg1	content					610:616	The G+C content	602:616	The G+C content of the genomic DNA	602:635	The G+C content of the genomic DNA was 58.2 mol%.
32125262	5	0	theme	58.2 mol	641:648	arg1	%					649:649	58.2 mol%	641:649	58.2 mol%	641:649	The G+C content of the genomic DNA was 58.2 mol%.
32125262	7	1	theme	DNA-DNA	1006:1012	arg1	values					1028:1033	Low DNA-DNA hybridization values	1002:1033	Low DNA-DNA hybridization values	1002:1033	Low DNA-DNA hybridization values showed that it formed a distinct genomic species.
32125262	4	2	theme	fatty	589:593	arg1	 0					531:532	 0	531:532	 0	531:532	Chemotaxonomic analysis showed that Q-8 was detected as the sole respiratory quinone and that iso-C15 : 0 and summed features 3, 8 and 9 were the major cellular fatty acids.
32125262	4	2	theme	fatty	589:593	arg1	features					545:552	summed features	538:552	summed features	538:552	Chemotaxonomic analysis showed that Q-8 was detected as the sole respiratory quinone and that iso-C15 : 0 and summed features 3, 8 and 9 were the major cellular fatty acids.
32125262	4	2	theme	fatty	589:593	arg1	acids					595:599	the major cellular fatty acids	570:599	the major cellular fatty acids	570:599	Chemotaxonomic analysis showed that Q-8 was detected as the sole respiratory quinone and that iso-C15 : 0 and summed features 3, 8 and 9 were the major cellular fatty acids.
32125262	2	3	dep	Gram-stain-negative	96:114	arg1	rod-shaped					126:135	rod-shaped	126:135	rod-shaped	126:135	A Gram-stain-negative, aerobic, rod-shaped bacterium with peritrichous flagella, designated strain HB161719T, was isolated from coastal sand collected from Tanmen Port in Hainan, PR China.
32125262	2	3	dep	Gram-stain-negative	96:114	arg1	aerobic					117:123	aerobic	117:123	aerobic	117:123	A Gram-stain-negative, aerobic, rod-shaped bacterium with peritrichous flagella, designated strain HB161719T, was isolated from coastal sand collected from Tanmen Port in Hainan, PR China.
32125262	7	4	theme	hybridization	1014:1026	arg1	values					1028:1033	Low DNA-DNA hybridization values	1002:1033	Low DNA-DNA hybridization values	1002:1033	Low DNA-DNA hybridization values showed that it formed a distinct genomic species.
32125262	6	5	theme	sequence	682:689	arg1	Analysis					652:659	Analysis	652:659	Analysis of the 16S rRNA gene sequence of the strain	652:703	Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus Microbulbifer, sharing 98.7, 98.4, 97.8 and 97.8 % sequence similarities to the closest relatives of Microbulbifer okinawensis ABABA23T, Microbulbifer pacificus SPO729T, Microbulbifer taiwanensis CC-LN1-12T and Microbulbifer gwangyangensis GY2T, respectively.
32125262	2	6	dep	China	276:280	arg1	PR					273:274	Hainan, PR China	265:280	PR	273:274	A Gram-stain-negative, aerobic, rod-shaped bacterium with peritrichous flagella, designated strain HB161719T, was isolated from coastal sand collected from Tanmen Port in Hainan, PR China.
32125262	4	7	theme	respiratory	493:503	arg1	Q-8					464:466	Q-8	464:466	Q-8	464:466	Chemotaxonomic analysis showed that Q-8 was detected as the sole respiratory quinone and that iso-C15 : 0 and summed features 3, 8 and 9 were the major cellular fatty acids.
32125262	4	7	theme	respiratory	493:503	arg1	quinone					505:511	the sole respiratory quinone	484:511	the sole respiratory quinone	484:511	Chemotaxonomic analysis showed that Q-8 was detected as the sole respiratory quinone and that iso-C15 : 0 and summed features 3, 8 and 9 were the major cellular fatty acids.
32125262	6	8	theme	GY2T	982:985	arg1	relatives					830:838	the closest relatives	818:838	the closest relatives of Microbulbifer okinawensis ABABA23T, Microbulbifer pacificus SPO729T, Microbulbifer taiwanensis CC-LN1-12T and Microbulbifer gwangyangensis GY2T	818:985	Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus Microbulbifer, sharing 98.7, 98.4, 97.8 and 97.8 % sequence similarities to the closest relatives of Microbulbifer okinawensis ABABA23T, Microbulbifer pacificus SPO729T, Microbulbifer taiwanensis CC-LN1-12T and Microbulbifer gwangyangensis GY2T, respectively.
32125262	4	9	theme	Chemotaxonomic	428:441	arg1	analysis					443:450	Chemotaxonomic analysis	428:450	Chemotaxonomic analysis	428:450	Chemotaxonomic analysis showed that Q-8 was detected as the sole respiratory quinone and that iso-C15 : 0 and summed features 3, 8 and 9 were the major cellular fatty acids.
32125262	6	10	theme	gene	677:680	arg1	sequence					682:689	the 16S rRNA gene sequence	664:689	the 16S rRNA gene sequence of the strain	664:703	Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus Microbulbifer, sharing 98.7, 98.4, 97.8 and 97.8 % sequence similarities to the closest relatives of Microbulbifer okinawensis ABABA23T, Microbulbifer pacificus SPO729T, Microbulbifer taiwanensis CC-LN1-12T and Microbulbifer gwangyangensis GY2T, respectively.
32125262	8	11	theme	Microbulbifer	1238:1250	arg1	sp					1262:1263	the name Microbulbifer harenosus sp	1229:1263	the name Microbulbifer harenosus sp	1229:1263	The combined phenotypic and molecular features supported that strain HB161719T represents a novel species of the genus Microbulbifer, for which the name Microbulbifer harenosus sp.
32125262	4	12	theme	summed	538:543	arg1	 0					531:532	 0	531:532	 0	531:532	Chemotaxonomic analysis showed that Q-8 was detected as the sole respiratory quinone and that iso-C15 : 0 and summed features 3, 8 and 9 were the major cellular fatty acids.
32125262	4	12	theme	summed	538:543	arg1	features					545:552	summed features	538:552	summed features	538:552	Chemotaxonomic analysis showed that Q-8 was detected as the sole respiratory quinone and that iso-C15 : 0 and summed features 3, 8 and 9 were the major cellular fatty acids.
32125262	4	12	theme	summed	538:543	arg1	acids					595:599	the major cellular fatty acids	570:599	the major cellular fatty acids	570:599	Chemotaxonomic analysis showed that Q-8 was detected as the sole respiratory quinone and that iso-C15 : 0 and summed features 3, 8 and 9 were the major cellular fatty acids.
32125262	7	13	theme	Low	1002:1004	arg1	values					1028:1033	Low DNA-DNA hybridization values	1002:1033	Low DNA-DNA hybridization values	1002:1033	Low DNA-DNA hybridization values showed that it formed a distinct genomic species.
32125262	6	14	theme	rRNA	672:675	arg1	sequence					682:689	the 16S rRNA gene sequence	664:689	the 16S rRNA gene sequence of the strain	664:703	Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus Microbulbifer, sharing 98.7, 98.4, 97.8 and 97.8 % sequence similarities to the closest relatives of Microbulbifer okinawensis ABABA23T, Microbulbifer pacificus SPO729T, Microbulbifer taiwanensis CC-LN1-12T and Microbulbifer gwangyangensis GY2T, respectively.
32125262	0	15	theme	Microbulbifer	0:12	arg1	sp					24:25	Microbulbifer harenosus sp	0:25	Microbulbifer harenosus sp.	0:26	Microbulbifer harenosus sp.
32125262	6	16	theme	closest	822:828	arg1	relatives					830:838	the closest relatives	818:838	the closest relatives of Microbulbifer okinawensis ABABA23T, Microbulbifer pacificus SPO729T, Microbulbifer taiwanensis CC-LN1-12T and Microbulbifer gwangyangensis GY2T	818:985	Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus Microbulbifer, sharing 98.7, 98.4, 97.8 and 97.8 % sequence similarities to the closest relatives of Microbulbifer okinawensis ABABA23T, Microbulbifer pacificus SPO729T, Microbulbifer taiwanensis CC-LN1-12T and Microbulbifer gwangyangensis GY2T, respectively.
32125262	3	17	theme	%	323:323	arg1	NaCl					331:334	2-11 % (w/v) NaCl	318:334	2-11 % (w/v) NaCl	318:334	The isolate was found to grow with 2-11 % (w/v) NaCl, at 15-45 °C and pH 6.0-10.0, with an optima of 2-3 % NaCl, 37 °C and pH 7.0, respectively.
32125262	2	18	theme	peritrichous	152:163	arg1	flagella					165:172	peritrichous flagella	152:172	peritrichous flagella	152:172	A Gram-stain-negative, aerobic, rod-shaped bacterium with peritrichous flagella, designated strain HB161719T, was isolated from coastal sand collected from Tanmen Port in Hainan, PR China.
32125262	3	19	theme	37 °C	396:400	arg1	optima					374:379	an optima	371:379	an optima of 2-3 % NaCl, 37 °C and pH 7.0, respectively	371:425	The isolate was found to grow with 2-11 % (w/v) NaCl, at 15-45 °C and pH 6.0-10.0, with an optima of 2-3 % NaCl, 37 °C and pH 7.0, respectively.
32125262	2	20	attach	isolated	208:215	arg2	bacterium					137:145	A Gram-stain-negative, aerobic, rod-shaped bacterium	94:145	A Gram-stain-negative, aerobic, rod-shaped bacterium	94:145	A Gram-stain-negative, aerobic, rod-shaped bacterium with peritrichous flagella, designated strain HB161719T, was isolated from coastal sand collected from Tanmen Port in Hainan, PR China.
32125262	2	20	attach	isolated	208:215	arg1	sand					230:233	coastal sand	222:233	coastal sand collected from Tanmen Port in Hainan, PR China	222:280	A Gram-stain-negative, aerobic, rod-shaped bacterium with peritrichous flagella, designated strain HB161719T, was isolated from coastal sand collected from Tanmen Port in Hainan, PR China.
32125262	5	21	theme	genomic	625:631	arg1	DNA					633:635	the genomic DNA	621:635	the genomic DNA	621:635	The G+C content of the genomic DNA was 58.2 mol%.
32125262	10	22	theme	=CGMCC	1314:1319	arg1	HB161719T					1303:1311	HB161719T	1303:1311	HB161719T (=CGMCC 1.13584T=JCM 32688T)	1303:1340	The type strain is HB161719T (=CGMCC 1.13584T=JCM 32688T).
32125262	10	22	theme	=CGMCC	1314:1319	arg1	32688T					1334:1339	=CGMCC 1.13584T=JCM 32688T	1314:1339	=CGMCC 1.13584T=JCM 32688T	1314:1339	The type strain is HB161719T (=CGMCC 1.13584T=JCM 32688T).
32125262	1	23	theme	coastal	80:86	arg1	sand					88:91	coastal sand	80:91	coastal sand	80:91	nov., an alginate-degrading bacterium isolated from coastal sand.
32125262	3	24	theme	2-3 	384:387	arg1	%					388:388	%	388:388	%	388:388	The isolate was found to grow with 2-11 % (w/v) NaCl, at 15-45 °C and pH 6.0-10.0, with an optima of 2-3 % NaCl, 37 °C and pH 7.0, respectively.
32125262	0	25	theme	harenosus	14:22	arg1	sp					24:25	Microbulbifer harenosus sp	0:25	Microbulbifer harenosus sp.	0:26	Microbulbifer harenosus sp.
32125262	6	26	theme	genus	736:740	arg1	Microbulbifer					742:754	the genus Microbulbifer	732:754	the genus Microbulbifer	732:754	Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus Microbulbifer, sharing 98.7, 98.4, 97.8 and 97.8 % sequence similarities to the closest relatives of Microbulbifer okinawensis ABABA23T, Microbulbifer pacificus SPO729T, Microbulbifer taiwanensis CC-LN1-12T and Microbulbifer gwangyangensis GY2T, respectively.
32125262	6	27	theme	CC-LN1-12T	938:947	arg1	relatives					830:838	the closest relatives	818:838	the closest relatives of Microbulbifer okinawensis ABABA23T, Microbulbifer pacificus SPO729T, Microbulbifer taiwanensis CC-LN1-12T and Microbulbifer gwangyangensis GY2T	818:985	Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus Microbulbifer, sharing 98.7, 98.4, 97.8 and 97.8 % sequence similarities to the closest relatives of Microbulbifer okinawensis ABABA23T, Microbulbifer pacificus SPO729T, Microbulbifer taiwanensis CC-LN1-12T and Microbulbifer gwangyangensis GY2T, respectively.
32125262	3	28	theme	15-45 °C and	340:351	arg1	pH					353:354	15-45 °C and pH 6.0-10.0	340:363	15-45 °C and pH 6.0-10.0	340:363	The isolate was found to grow with 2-11 % (w/v) NaCl, at 15-45 °C and pH 6.0-10.0, with an optima of 2-3 % NaCl, 37 °C and pH 7.0, respectively.
32125262	8	29	theme	strain	1147:1152	arg1	HB161719T					1154:1162	strain HB161719T	1147:1162	strain HB161719T	1147:1162	The combined phenotypic and molecular features supported that strain HB161719T represents a novel species of the genus Microbulbifer, for which the name Microbulbifer harenosus sp.
32125262	8	30	theme	combined	1089:1096	arg1	features					1123:1130	The combined phenotypic and molecular features	1085:1130	The combined phenotypic and molecular features	1085:1130	The combined phenotypic and molecular features supported that strain HB161719T represents a novel species of the genus Microbulbifer, for which the name Microbulbifer harenosus sp.
32125262	8	31	theme	genus	1198:1202	arg1	Microbulbifer					1204:1216	the genus Microbulbifer	1194:1216	the genus Microbulbifer	1194:1216	The combined phenotypic and molecular features supported that strain HB161719T represents a novel species of the genus Microbulbifer, for which the name Microbulbifer harenosus sp.
32125262	6	32	theme	16S	668:670	arg1	rRNA					672:675	the 16S rRNA	664:675	the 16S rRNA gene sequence of the strain	664:703	Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus Microbulbifer, sharing 98.7, 98.4, 97.8 and 97.8 % sequence similarities to the closest relatives of Microbulbifer okinawensis ABABA23T, Microbulbifer pacificus SPO729T, Microbulbifer taiwanensis CC-LN1-12T and Microbulbifer gwangyangensis GY2T, respectively.
32125262	4	33	theme	sole	488:491	arg1	Q-8					464:466	Q-8	464:466	Q-8	464:466	Chemotaxonomic analysis showed that Q-8 was detected as the sole respiratory quinone and that iso-C15 : 0 and summed features 3, 8 and 9 were the major cellular fatty acids.
32125262	4	33	theme	sole	488:491	arg1	quinone					505:511	the sole respiratory quinone	484:511	the sole respiratory quinone	484:511	Chemotaxonomic analysis showed that Q-8 was detected as the sole respiratory quinone and that iso-C15 : 0 and summed features 3, 8 and 9 were the major cellular fatty acids.
32125262	4	34	theme	cellular	580:587	arg1	 0					531:532	 0	531:532	 0	531:532	Chemotaxonomic analysis showed that Q-8 was detected as the sole respiratory quinone and that iso-C15 : 0 and summed features 3, 8 and 9 were the major cellular fatty acids.
32125262	4	34	theme	cellular	580:587	arg1	features					545:552	summed features	538:552	summed features	538:552	Chemotaxonomic analysis showed that Q-8 was detected as the sole respiratory quinone and that iso-C15 : 0 and summed features 3, 8 and 9 were the major cellular fatty acids.
32125262	4	34	theme	cellular	580:587	arg1	acids					595:599	the major cellular fatty acids	570:599	the major cellular fatty acids	570:599	Chemotaxonomic analysis showed that Q-8 was detected as the sole respiratory quinone and that iso-C15 : 0 and summed features 3, 8 and 9 were the major cellular fatty acids.
32125262	2	35	with	bacterium	137:145	arg1	flagella					165:172	peritrichous flagella	152:172	peritrichous flagella	152:172	A Gram-stain-negative, aerobic, rod-shaped bacterium with peritrichous flagella, designated strain HB161719T, was isolated from coastal sand collected from Tanmen Port in Hainan, PR China.
32125262	6	36	theme	sequence	793:800	arg1	similarities					802:813	98.7, 98.4, 97.8 and 97.8 % sequence similarities	765:813	98.7, 98.4, 97.8 and 97.8 % sequence similarities to the closest relatives of Microbulbifer okinawensis ABABA23T, Microbulbifer pacificus SPO729T, Microbulbifer taiwanensis CC-LN1-12T and Microbulbifer gwangyangensis GY2T	765:985	Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus Microbulbifer, sharing 98.7, 98.4, 97.8 and 97.8 % sequence similarities to the closest relatives of Microbulbifer okinawensis ABABA23T, Microbulbifer pacificus SPO729T, Microbulbifer taiwanensis CC-LN1-12T and Microbulbifer gwangyangensis GY2T, respectively.
32125262	2	37	theme	strain	186:191	arg1	HB161719T					193:201	strain HB161719T	186:201	strain HB161719T	186:201	A Gram-stain-negative, aerobic, rod-shaped bacterium with peritrichous flagella, designated strain HB161719T, was isolated from coastal sand collected from Tanmen Port in Hainan, PR China.
32125262	3	38	theme	%	388:388	arg1	 NaCl					389:393	2-3 % NaCl	384:393	2-3 % NaCl	384:393	The isolate was found to grow with 2-11 % (w/v) NaCl, at 15-45 °C and pH 6.0-10.0, with an optima of 2-3 % NaCl, 37 °C and pH 7.0, respectively.
32125262	6	39	with	affiliation	715:725	arg1	Microbulbifer					742:754	the genus Microbulbifer	732:754	the genus Microbulbifer	732:754	Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus Microbulbifer, sharing 98.7, 98.4, 97.8 and 97.8 % sequence similarities to the closest relatives of Microbulbifer okinawensis ABABA23T, Microbulbifer pacificus SPO729T, Microbulbifer taiwanensis CC-LN1-12T and Microbulbifer gwangyangensis GY2T, respectively.
32125262	2	40	theme	Gram-stain-negative	96:114	arg1	bacterium					137:145	A Gram-stain-negative, aerobic, rod-shaped bacterium	94:145	A Gram-stain-negative, aerobic, rod-shaped bacterium	94:145	A Gram-stain-negative, aerobic, rod-shaped bacterium with peritrichous flagella, designated strain HB161719T, was isolated from coastal sand collected from Tanmen Port in Hainan, PR China.
32125262	5	41	theme	G+C	606:608	arg1	content					610:616	The G+C content	602:616	The G+C content of the genomic DNA	602:635	The G+C content of the genomic DNA was 58.2 mol%.
32125262	5	41	theme	G+C	606:608	arg1	%					649:649	58.2 mol%	641:649	58.2 mol%	641:649	The G+C content of the genomic DNA was 58.2 mol%.
32125262	6	42	dep	%	791:791	arg1	97.8 					786:790	97.8 	786:790	97.8 	786:790	Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus Microbulbifer, sharing 98.7, 98.4, 97.8 and 97.8 % sequence similarities to the closest relatives of Microbulbifer okinawensis ABABA23T, Microbulbifer pacificus SPO729T, Microbulbifer taiwanensis CC-LN1-12T and Microbulbifer gwangyangensis GY2T, respectively.
32125262	6	42	dep	%	791:791	arg1	97.8					777:780	97.8	777:780	97.8	777:780	Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus Microbulbifer, sharing 98.7, 98.4, 97.8 and 97.8 % sequence similarities to the closest relatives of Microbulbifer okinawensis ABABA23T, Microbulbifer pacificus SPO729T, Microbulbifer taiwanensis CC-LN1-12T and Microbulbifer gwangyangensis GY2T, respectively.
32125262	3	43	theme	 NaCl	389:393	arg1	optima					374:379	an optima	371:379	an optima of 2-3 % NaCl, 37 °C and pH 7.0, respectively	371:425	The isolate was found to grow with 2-11 % (w/v) NaCl, at 15-45 °C and pH 6.0-10.0, with an optima of 2-3 % NaCl, 37 °C and pH 7.0, respectively.
32125262	10	44	theme	1.13584T=JCM	1321:1332	arg1	HB161719T					1303:1311	HB161719T	1303:1311	HB161719T (=CGMCC 1.13584T=JCM 32688T)	1303:1340	The type strain is HB161719T (=CGMCC 1.13584T=JCM 32688T).
32125262	10	44	theme	1.13584T=JCM	1321:1332	arg1	32688T					1334:1339	=CGMCC 1.13584T=JCM 32688T	1314:1339	=CGMCC 1.13584T=JCM 32688T	1314:1339	The type strain is HB161719T (=CGMCC 1.13584T=JCM 32688T).
32125262	3	45	dep	%	323:323	arg1	w/v					326:328	w/v	326:328	w/v	326:328	The isolate was found to grow with 2-11 % (w/v) NaCl, at 15-45 °C and pH 6.0-10.0, with an optima of 2-3 % NaCl, 37 °C and pH 7.0, respectively.
32125262	6	46	theme	strain	698:703	arg1	sequence					682:689	the 16S rRNA gene sequence	664:689	the 16S rRNA gene sequence of the strain	664:703	Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus Microbulbifer, sharing 98.7, 98.4, 97.8 and 97.8 % sequence similarities to the closest relatives of Microbulbifer okinawensis ABABA23T, Microbulbifer pacificus SPO729T, Microbulbifer taiwanensis CC-LN1-12T and Microbulbifer gwangyangensis GY2T, respectively.
32125262	6	47	dep	similarities	802:813	arg1	%					791:791	%	791:791	%	791:791	Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus Microbulbifer, sharing 98.7, 98.4, 97.8 and 97.8 % sequence similarities to the closest relatives of Microbulbifer okinawensis ABABA23T, Microbulbifer pacificus SPO729T, Microbulbifer taiwanensis CC-LN1-12T and Microbulbifer gwangyangensis GY2T, respectively.
32125262	2	48	theme	coastal	222:228	arg1	sand					230:233	coastal sand	222:233	coastal sand collected from Tanmen Port in Hainan, PR China	222:280	A Gram-stain-negative, aerobic, rod-shaped bacterium with peritrichous flagella, designated strain HB161719T, was isolated from coastal sand collected from Tanmen Port in Hainan, PR China.
32125262	4	49	dep	 0	531:532	arg1	3					554:554	3	554:554	3	554:554	Chemotaxonomic analysis showed that Q-8 was detected as the sole respiratory quinone and that iso-C15 : 0 and summed features 3, 8 and 9 were the major cellular fatty acids.
32125262	4	49	dep	 0	531:532	arg1	9					563:563	9	563:563	9	563:563	Chemotaxonomic analysis showed that Q-8 was detected as the sole respiratory quinone and that iso-C15 : 0 and summed features 3, 8 and 9 were the major cellular fatty acids.
32125262	2	50	theme	Hainan	265:270	arg1	China					276:280	Hainan, PR China	265:280	China	276:280	A Gram-stain-negative, aerobic, rod-shaped bacterium with peritrichous flagella, designated strain HB161719T, was isolated from coastal sand collected from Tanmen Port in Hainan, PR China.
32125262	10	51	theme	type	1288:1291	arg1	HB161719T					1303:1311	HB161719T	1303:1311	HB161719T (=CGMCC 1.13584T=JCM 32688T)	1303:1340	The type strain is HB161719T (=CGMCC 1.13584T=JCM 32688T).
32125262	10	51	theme	type	1288:1291	arg1	strain					1293:1298	The type strain	1284:1298	The type strain	1284:1298	The type strain is HB161719T (=CGMCC 1.13584T=JCM 32688T).
32125262	8	52	theme	Microbulbifer	1204:1216	arg1	species					1183:1189	a novel species	1175:1189	a novel species	1175:1189	The combined phenotypic and molecular features supported that strain HB161719T represents a novel species of the genus Microbulbifer, for which the name Microbulbifer harenosus sp.
32125262	8	53	theme	harenosus	1252:1260	arg1	sp					1262:1263	the name Microbulbifer harenosus sp	1229:1263	the name Microbulbifer harenosus sp	1229:1263	The combined phenotypic and molecular features supported that strain HB161719T represents a novel species of the genus Microbulbifer, for which the name Microbulbifer harenosus sp.
32125262	8	54	theme	molecular	1113:1121	arg1	features					1123:1130	The combined phenotypic and molecular features	1085:1130	The combined phenotypic and molecular features	1085:1130	The combined phenotypic and molecular features supported that strain HB161719T represents a novel species of the genus Microbulbifer, for which the name Microbulbifer harenosus sp.
32125262	3	55	theme	pH	406:407	arg1	optima					374:379	an optima	371:379	an optima of 2-3 % NaCl, 37 °C and pH 7.0, respectively	371:425	The isolate was found to grow with 2-11 % (w/v) NaCl, at 15-45 °C and pH 6.0-10.0, with an optima of 2-3 % NaCl, 37 °C and pH 7.0, respectively.
32125262	8	56	theme	name	1233:1236	arg1	sp					1262:1263	the name Microbulbifer harenosus sp	1229:1263	the name Microbulbifer harenosus sp	1229:1263	The combined phenotypic and molecular features supported that strain HB161719T represents a novel species of the genus Microbulbifer, for which the name Microbulbifer harenosus sp.
32125262	8	57	theme	phenotypic	1098:1107	arg1	features					1123:1130	The combined phenotypic and molecular features	1085:1130	The combined phenotypic and molecular features	1085:1130	The combined phenotypic and molecular features supported that strain HB161719T represents a novel species of the genus Microbulbifer, for which the name Microbulbifer harenosus sp.
32125262	5	58	theme	DNA	633:635	arg1	content					610:616	The G+C content	602:616	The G+C content of the genomic DNA	602:635	The G+C content of the genomic DNA was 58.2 mol%.
32125262	5	58	theme	DNA	633:635	arg1	%					649:649	58.2 mol%	641:649	58.2 mol%	641:649	The G+C content of the genomic DNA was 58.2 mol%.
32125262	8	59	theme	novel	1177:1181	arg1	species					1183:1189	a novel species	1175:1189	a novel species	1175:1189	The combined phenotypic and molecular features supported that strain HB161719T represents a novel species of the genus Microbulbifer, for which the name Microbulbifer harenosus sp.
32125262	4	60	theme	major	574:578	arg1	 0					531:532	 0	531:532	 0	531:532	Chemotaxonomic analysis showed that Q-8 was detected as the sole respiratory quinone and that iso-C15 : 0 and summed features 3, 8 and 9 were the major cellular fatty acids.
32125262	4	60	theme	major	574:578	arg1	features					545:552	summed features	538:552	summed features	538:552	Chemotaxonomic analysis showed that Q-8 was detected as the sole respiratory quinone and that iso-C15 : 0 and summed features 3, 8 and 9 were the major cellular fatty acids.
32125262	4	60	theme	major	574:578	arg1	acids					595:599	the major cellular fatty acids	570:599	the major cellular fatty acids	570:599	Chemotaxonomic analysis showed that Q-8 was detected as the sole respiratory quinone and that iso-C15 : 0 and summed features 3, 8 and 9 were the major cellular fatty acids.
32125262	6	61	theme	ABABA23T	869:876	arg1	relatives					830:838	the closest relatives	818:838	the closest relatives of Microbulbifer okinawensis ABABA23T, Microbulbifer pacificus SPO729T, Microbulbifer taiwanensis CC-LN1-12T and Microbulbifer gwangyangensis GY2T	818:985	Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus Microbulbifer, sharing 98.7, 98.4, 97.8 and 97.8 % sequence similarities to the closest relatives of Microbulbifer okinawensis ABABA23T, Microbulbifer pacificus SPO729T, Microbulbifer taiwanensis CC-LN1-12T and Microbulbifer gwangyangensis GY2T, respectively.
32125262	3	62	theme	2-11 	318:322	arg1	%					323:323	%	323:323	%	323:323	The isolate was found to grow with 2-11 % (w/v) NaCl, at 15-45 °C and pH 6.0-10.0, with an optima of 2-3 % NaCl, 37 °C and pH 7.0, respectively.
32125262	1	63	theme	alginate-degrading	37:54	arg1	bacterium					56:64	an alginate-degrading bacterium	34:64	an alginate-degrading bacterium	34:64	nov., an alginate-degrading bacterium isolated from coastal sand.
32125262	1	63	theme	alginate-degrading	37:54	arg1	nov.					28:31	nov.	28:31	nov.	28:31	nov., an alginate-degrading bacterium isolated from coastal sand.
32125262	7	64	theme	distinct	1059:1066	arg1	species					1076:1082	a distinct genomic species	1057:1082	a distinct genomic species	1057:1082	Low DNA-DNA hybridization values showed that it formed a distinct genomic species.
32125262	7	65	theme	genomic	1068:1074	arg1	species					1076:1082	a distinct genomic species	1057:1082	a distinct genomic species	1057:1082	Low DNA-DNA hybridization values showed that it formed a distinct genomic species.
33774520	1	0	theme	species	152:158	arg1	composition					111:121	Nutritional and biochemical composition	83:121	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin)	83:264	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	4	1	theme	high	737:740	arg1	quantum					742:748	a significantly high quantum	721:748	a significantly high quantum of phenolics and antioxidant potential	721:787	H. polyrhizus have a significantly high quantum of phenolics and antioxidant potential than H. undatus.
33774520	5	2	theme	0.70-1.5 mg	886:896	arg1	Fe					898:899	0.70-1.5 mg Fe	886:899	0.70-1.5 mg Fe	886:899	100 g fruit contained about 120-200 mg K, 30-45 mg Mg, 20-45 mg Ca, 20-35 mg P, 0.70-1.5 mg Fe, and 0.20-0.40 mg Zn.
33774520	1	3	dep	H.	380:381	arg1	undatus					383:389	H. undatus	380:389	H. undatus	380:389	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	4	4	theme	H.	794:795	arg1	undatus					797:803	H. undatus	794:803	H. undatus	794:803	H. polyrhizus have a significantly high quantum of phenolics and antioxidant potential than H. undatus.
33774520	7	5	theme	low	1106:1108	arg1	calories					1110:1117	low calories	1106:1117	low calories	1106:1117	It is an ideal fruit for maintaining good health as it has low calories.
33774520	5	6	theme	100 g	806:810	arg1	fruit					812:816	100 g fruit	806:816	100 g fruit	806:816	100 g fruit contained about 120-200 mg K, 30-45 mg Mg, 20-45 mg Ca, 20-35 mg P, 0.70-1.5 mg Fe, and 0.20-0.40 mg Zn.
33774520	1	7	dep	species	152:158	arg1	fruits					230:235	fruits	230:235	fruits with red pulp and pink skin	230:263	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	1	7	dep	species	152:158	arg1	species					152:158	dragon fruit (Hylocereus) species	126:158	dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin)	126:264	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	1	7	dep	species	152:158	arg1	H.					215:216	H.	215:216	H.	215:216	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	1	7	dep	species	152:158	arg1	H.					160:161	H.	160:161	H.	160:161	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	1	8	theme	nutritional	299:309	arg1	data					323:326	the nutritional composition data	295:326	the nutritional composition data for dragon fruit	295:343	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	6	9	theme	Vitamin	923:929	arg1	C					931:931	Vitamin C	923:931	Vitamin C	923:931	Vitamin C was found maximum (6 mg/100 g), followed by vitamin E (150 µg), pantothenic acid (50 µg), and vitamin K1 (25 µg).
33774520	3	10	theme	15-35 mg	665:672	arg1	CE					674:675	55 mg GAE and 15-35 mg CE	651:675	CE	674:675	Total phenolics and flavonoids content varied between 25 and 55 mg GAE and 15-35 mg CE per 100 g, respectively.
33774520	1	11	theme	composition	311:321	arg1	data					323:326	the nutritional composition data	295:326	the nutritional composition data for dragon fruit	295:343	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	5	12	contain	contained	818:826	arg2	Zn					919:920	0.20-0.40 mg Zn	906:920	0.20-0.40 mg Zn	906:920	100 g fruit contained about 120-200 mg K, 30-45 mg Mg, 20-45 mg Ca, 20-35 mg P, 0.70-1.5 mg Fe, and 0.20-0.40 mg Zn.
33774520	5	12	contain	contained	818:826	arg1	fruit					812:816	100 g fruit	806:816	100 g fruit	806:816	100 g fruit contained about 120-200 mg K, 30-45 mg Mg, 20-45 mg Ca, 20-35 mg P, 0.70-1.5 mg Fe, and 0.20-0.40 mg Zn.
33774520	5	12	contain	contained	818:826	arg2	Ca					870:871	about 120-200 mg K, 30-45 mg Mg, 20-45 mg Ca	828:871	about 120-200 mg K, 30-45 mg Mg, 20-45 mg Ca	828:871	100 g fruit contained about 120-200 mg K, 30-45 mg Mg, 20-45 mg Ca, 20-35 mg P, 0.70-1.5 mg Fe, and 0.20-0.40 mg Zn.
33774520	5	12	contain	contained	818:826	arg2	Fe					898:899	0.70-1.5 mg Fe	886:899	0.70-1.5 mg Fe	886:899	100 g fruit contained about 120-200 mg K, 30-45 mg Mg, 20-45 mg Ca, 20-35 mg P, 0.70-1.5 mg Fe, and 0.20-0.40 mg Zn.
33774520	5	12	contain	contained	818:826	arg2	P					883:883	20-35 mg P	874:883	20-35 mg P	874:883	100 g fruit contained about 120-200 mg K, 30-45 mg Mg, 20-45 mg Ca, 20-35 mg P, 0.70-1.5 mg Fe, and 0.20-0.40 mg Zn.
33774520	7	13	theme	good	1084:1087	arg1	health					1089:1094	good health as it has low calories	1084:1117	good health as it has low calories	1084:1117	It is an ideal fruit for maintaining good health as it has low calories.
33774520	4	14	dep	H.	702:703	arg1	polyrhizus					705:714	H. polyrhizus	702:714	H. polyrhizus	702:714	H. polyrhizus have a significantly high quantum of phenolics and antioxidant potential than H. undatus.
33774520	6	15	theme	vitamin	977:983	arg1	E					985:985	vitamin E	977:985	vitamin E (150 µg)	977:994	Vitamin C was found maximum (6 mg/100 g), followed by vitamin E (150 µg), pantothenic acid (50 µg), and vitamin K1 (25 µg).
33774520	6	15	theme	vitamin	977:983	arg1	150 µg					988:993	150 µg	988:993	150 µg	988:993	Vitamin C was found maximum (6 mg/100 g), followed by vitamin E (150 µg), pantothenic acid (50 µg), and vitamin K1 (25 µg).
33774520	6	16	theme	vitamin	1027:1033	arg1	K1					1035:1036	vitamin K1	1027:1036	vitamin K1 (25 µg)	1027:1044	Vitamin C was found maximum (6 mg/100 g), followed by vitamin E (150 µg), pantothenic acid (50 µg), and vitamin K1 (25 µg).
33774520	6	16	theme	vitamin	1027:1033	arg1	25 µg					1039:1043	25 µg	1039:1043	25 µg	1039:1043	Vitamin C was found maximum (6 mg/100 g), followed by vitamin E (150 µg), pantothenic acid (50 µg), and vitamin K1 (25 µg).
33774520	1	17	theme	Nutritional	83:93	arg1	composition					111:121	Nutritional and biochemical composition	83:121	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin)	83:264	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	0	18	theme	Biochemical	0:10	arg1	characterization					28:43	Biochemical and nutritional characterization	0:43	Biochemical and nutritional characterization of dragon fruit (Hylocereus species).	0:81	Biochemical and nutritional characterization of dragon fruit (Hylocereus species).
33774520	3	19	theme	phenolics	596:604	arg1	content					621:627	Total phenolics and flavonoids content	590:627	Total phenolics and flavonoids content	590:627	Total phenolics and flavonoids content varied between 25 and 55 mg GAE and 15-35 mg CE per 100 g, respectively.
33774520	1	20	dep	H.	160:161	arg1	fruits					172:177	fruits	172:177	fruits with white pulp and pink skin	172:207	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	1	20	dep	H.	160:161	arg1	undatus					163:169	H. undatus (fruits with white pulp and pink skin)	160:208	H. undatus (fruits with white pulp and pink skin)	160:208	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	1	21	theme	red	242:244	arg1	pulp					246:249	red pulp	242:249	red pulp	242:249	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	1	22	theme	biochemical	99:109	arg1	composition					111:121	Nutritional and biochemical composition	83:121	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin)	83:264	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	1	23	theme	white	184:188	arg1	pulp					190:193	white pulp	184:193	white pulp	184:193	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	0	24	theme	nutritional	16:26	arg1	characterization					28:43	Biochemical and nutritional characterization	0:43	Biochemical and nutritional characterization of dragon fruit (Hylocereus species).	0:81	Biochemical and nutritional characterization of dragon fruit (Hylocereus species).
33774520	4	25	theme	phenolics	753:761	arg1	quantum					742:748	a significantly high quantum	721:748	a significantly high quantum of phenolics and antioxidant potential	721:787	H. polyrhizus have a significantly high quantum of phenolics and antioxidant potential than H. undatus.
33774520	1	26	theme	dragon	332:337	arg1	fruit					339:343	dragon fruit	332:343	dragon fruit	332:343	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	0	27	theme	dragon	48:53	arg1	fruit					55:59	dragon fruit	48:59	dragon fruit (Hylocereus species)	48:80	Biochemical and nutritional characterization of dragon fruit (Hylocereus species).
33774520	0	27	theme	dragon	48:53	arg1	species					73:79	Hylocereus species	62:79	Hylocereus species	62:79	Biochemical and nutritional characterization of dragon fruit (Hylocereus species).
33774520	3	28	theme	Total	590:594	arg1	content					621:627	Total phenolics and flavonoids content	590:627	Total phenolics and flavonoids content	590:627	Total phenolics and flavonoids content varied between 25 and 55 mg GAE and 15-35 mg CE per 100 g, respectively.
33774520	4	29	theme	potential	779:787	arg1	quantum					742:748	a significantly high quantum	721:748	a significantly high quantum of phenolics and antioxidant potential	721:787	H. polyrhizus have a significantly high quantum of phenolics and antioxidant potential than H. undatus.
33774520	5	30	dep	120-200 mg K	834:845	arg1	30-45 mg Mg					848:858	30-45 mg Mg	848:858	30-45 mg Mg	848:858	100 g fruit contained about 120-200 mg K, 30-45 mg Mg, 20-45 mg Ca, 20-35 mg P, 0.70-1.5 mg Fe, and 0.20-0.40 mg Zn.
33774520	5	30	dep	120-200 mg K	834:845	arg1	20-45 mg					861:868	20-45 mg	861:868	20-45 mg	861:868	100 g fruit contained about 120-200 mg K, 30-45 mg Mg, 20-45 mg Ca, 20-35 mg P, 0.70-1.5 mg Fe, and 0.20-0.40 mg Zn.
33774520	1	31	theme	pink	255:258	arg1	skin					260:263	pink skin	255:263	pink skin	255:263	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	4	32	theme	antioxidant	767:777	arg1	potential					779:787	antioxidant potential	767:787	antioxidant potential	767:787	H. polyrhizus have a significantly high quantum of phenolics and antioxidant potential than H. undatus.
33774520	5	33	theme	0.20-0.40 mg	906:917	arg1	Zn					919:920	0.20-0.40 mg Zn	906:920	0.20-0.40 mg Zn	906:920	100 g fruit contained about 120-200 mg K, 30-45 mg Mg, 20-45 mg Ca, 20-35 mg P, 0.70-1.5 mg Fe, and 0.20-0.40 mg Zn.
33774520	1	34	theme	pink	199:202	arg1	skin					204:207	pink skin	199:207	pink skin	199:207	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	1	35	with	fruits	172:177	arg1	pulp					190:193	white pulp	184:193	white pulp	184:193	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	1	35	with	fruits	172:177	arg1	skin					204:207	pink skin	199:207	pink skin	199:207	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	2	36	theme	total	423:427	arg1	sugar					429:433	total sugar	423:433	total sugar	423:433	The pH, TSS, total sugar, moisture, ash, protein, and dietary fibre content varied between 4.8 and 5.4, 8-12%, 5.13-7.06%, 82-85%, 0.7-0.85%, 0.90-1.1% and 0.8-1.0%, respectively.
33774520	1	37	with	fruits	230:235	arg1	pulp					246:249	red pulp	242:249	red pulp	242:249	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	1	37	with	fruits	230:235	arg1	skin					260:263	pink skin	255:263	pink skin	255:263	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	0	38	theme	fruit	55:59	arg1	characterization					28:43	Biochemical and nutritional characterization	0:43	Biochemical and nutritional characterization of dragon fruit (Hylocereus species).	0:81	Biochemical and nutritional characterization of dragon fruit (Hylocereus species).
33774520	3	39	theme	flavonoids	610:619	arg1	content					621:627	Total phenolics and flavonoids content	590:627	Total phenolics and flavonoids content	590:627	Total phenolics and flavonoids content varied between 25 and 55 mg GAE and 15-35 mg CE per 100 g, respectively.
33774520	5	40	theme	20-35 mg	874:881	arg1	P					883:883	20-35 mg P	874:883	20-35 mg P	874:883	100 g fruit contained about 120-200 mg K, 30-45 mg Mg, 20-45 mg Ca, 20-35 mg P, 0.70-1.5 mg Fe, and 0.20-0.40 mg Zn.
33774520	1	41	theme	dragon	126:131	arg1	species					152:158	dragon fruit (Hylocereus) species	126:158	dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin)	126:264	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	1	41	theme	dragon	126:131	arg1	H.					215:216	H.	215:216	H.	215:216	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	1	41	theme	dragon	126:131	arg1	H.					160:161	H.	160:161	H.	160:161	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	1	42	dep	H.	395:396	arg1	polyrhizus					398:407	H. polyrhizus	395:407	H. polyrhizus	395:407	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	2	43	theme	fibre	472:476	arg1	content					478:484	dietary fibre content	464:484	dietary fibre content	464:484	The pH, TSS, total sugar, moisture, ash, protein, and dietary fibre content varied between 4.8 and 5.4, 8-12%, 5.13-7.06%, 82-85%, 0.7-0.85%, 0.90-1.1% and 0.8-1.0%, respectively.
33774520	5	44	theme	120-200 mg K	834:845	arg1	Ca					870:871	about 120-200 mg K, 30-45 mg Mg, 20-45 mg Ca	828:871	about 120-200 mg K, 30-45 mg Mg, 20-45 mg Ca	828:871	100 g fruit contained about 120-200 mg K, 30-45 mg Mg, 20-45 mg Ca, 20-35 mg P, 0.70-1.5 mg Fe, and 0.20-0.40 mg Zn.
33774520	1	45	theme	fruit	133:137	arg1	species					152:158	dragon fruit (Hylocereus) species	126:158	dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin)	126:264	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	1	45	theme	fruit	133:137	arg1	H.					215:216	H.	215:216	H.	215:216	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	1	45	theme	fruit	133:137	arg1	H.					160:161	H.	160:161	H.	160:161	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	3	46	theme	55 mg	651:655	arg1	GAE					657:659	55 mg GAE and 15-35 mg CE	651:675	GAE	657:659	Total phenolics and flavonoids content varied between 25 and 55 mg GAE and 15-35 mg CE per 100 g, respectively.
33774520	2	47	theme	dietary	464:470	arg1	content					478:484	dietary fibre content	464:484	dietary fibre content	464:484	The pH, TSS, total sugar, moisture, ash, protein, and dietary fibre content varied between 4.8 and 5.4, 8-12%, 5.13-7.06%, 82-85%, 0.7-0.85%, 0.90-1.1% and 0.8-1.0%, respectively.
33774520	1	48	dep	H.	215:216	arg1	polyrhizus					218:227	H. polyrhizus	215:227	H. polyrhizus	215:227	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	6	49	theme	pantothenic	997:1007	arg1	50 µg					1015:1019	50 µg	1015:1019	50 µg	1015:1019	Vitamin C was found maximum (6 mg/100 g), followed by vitamin E (150 µg), pantothenic acid (50 µg), and vitamin K1 (25 µg).
33774520	6	49	theme	pantothenic	997:1007	arg1	acid					1009:1012	pantothenic acid	997:1012	pantothenic acid (50 µg)	997:1020	Vitamin C was found maximum (6 mg/100 g), followed by vitamin E (150 µg), pantothenic acid (50 µg), and vitamin K1 (25 µg).
33774520	7	50	theme	ideal	1056:1060	arg1	fruit					1062:1066	an ideal fruit	1053:1066	an ideal fruit for maintaining good health as it has low calories	1053:1117	It is an ideal fruit for maintaining good health as it has low calories.
33774520	7	50	theme	ideal	1056:1060	arg1	It					1047:1048	It	1047:1048	It	1047:1048	It is an ideal fruit for maintaining good health as it has low calories.
33774520	1	51	theme	Hylocereus	140:149	arg1	species					152:158	dragon fruit (Hylocereus) species	126:158	dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin)	126:264	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	1	51	theme	Hylocereus	140:149	arg1	H.					215:216	H.	215:216	H.	215:216	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	1	51	theme	Hylocereus	140:149	arg1	H.					160:161	H.	160:161	H.	160:161	Nutritional and biochemical composition of dragon fruit (Hylocereus) species H. undatus (fruits with white pulp and pink skin), and H. polyrhizus (fruits with red pulp and pink skin) were investigated to develop the nutritional composition data for dragon fruit and compare the difference between H. undatus and H. polyrhizus.
33774520	0	52	theme	Hylocereus	62:71	arg1	fruit					55:59	dragon fruit	48:59	dragon fruit (Hylocereus species)	48:80	Biochemical and nutritional characterization of dragon fruit (Hylocereus species).
33774520	0	52	theme	Hylocereus	62:71	arg1	species					73:79	Hylocereus species	62:79	Hylocereus species	62:79	Biochemical and nutritional characterization of dragon fruit (Hylocereus species).
33774520	4	53	contain	have	716:719	arg1	H.					702:703	H.	702:703	H.	702:703	H. polyrhizus have a significantly high quantum of phenolics and antioxidant potential than H. undatus.
33774520	4	53	contain	have	716:719	arg2	quantum					742:748	a significantly high quantum	721:748	a significantly high quantum of phenolics and antioxidant potential	721:787	H. polyrhizus have a significantly high quantum of phenolics and antioxidant potential than H. undatus.
33774520	7	54	contain	has	1102:1104	arg1	it					1099:1100	it	1099:1100	it	1099:1100	It is an ideal fruit for maintaining good health as it has low calories.
33774520	7	54	contain	has	1102:1104	arg2	calories					1110:1117	low calories	1106:1117	low calories	1106:1117	It is an ideal fruit for maintaining good health as it has low calories.
34890843	7	0	theme	infected	1525:1532	arg1	donors					1548:1553	infected vs uninfected donors	1525:1553	infected vs uninfected donors	1525:1553	In line with these findings, fecal microbiome transplantation of germ-free recipient mice using infected vs uninfected donors showed altered expression of several tight-junction proteins and increased susceptibility to dextran sodium sulfate-induced colitis.
34890843	6	1	theme	several	1284:1290	arg1	genes					1292:1296	several genes	1284:1296	several genes affecting intestinal epithelial homeostasis	1284:1340	Gut microbiome composition, including changes in the mucin-degrading bacteria, Akkermansia muciniphila and Ruminococcus gnavus, was altered significantly, as was expression of several genes affecting intestinal epithelial homeostasis in isolated colonoids, after C tropicalis infection compared with uninfected controls.
34890843	8	2	theme	mucin-degrading	1772:1786	arg1	bacteria					1788:1795	mucin-degrading bacteria	1772:1795	mucin-degrading bacteria	1772:1795	CONCLUSIONS C tropicalis induces dysbiosis that involves changes in the presence of mucin-degrading bacteria, leading to altered tight junction protein expression with increased intestinal permeability and followed by induction of robust Th1/Th17 responses, which ultimately lead to an accelerated proinflammatory phenotype in experimental colitic mice.
34890843	1	3	theme	first-degree	257:268	arg1	relatives					270:278	first-degree relatives	257:278	first-degree relatives without Crohn's disease	257:302	BACKGROUND & AIMS We previously showed that abundance of Candida tropicalis is significantly greater in Crohn's disease patients compared with first-degree relatives without Crohn's disease.
34890843	7	4	theme	mice	1514:1517	arg1	transplantation					1475:1489	fecal microbiome transplantation	1458:1489	fecal microbiome transplantation of germ-free recipient mice using infected vs uninfected donors	1458:1553	In line with these findings, fecal microbiome transplantation of germ-free recipient mice using infected vs uninfected donors showed altered expression of several tight-junction proteins and increased susceptibility to dextran sodium sulfate-induced colitis.
34890843	7	5	theme	germ-free	1494:1502	arg1	mice					1514:1517	germ-free recipient mice	1494:1517	germ-free recipient mice using infected vs uninfected donors	1494:1553	In line with these findings, fecal microbiome transplantation of germ-free recipient mice using infected vs uninfected donors showed altered expression of several tight-junction proteins and increased susceptibility to dextran sodium sulfate-induced colitis.
34890843	6	6	theme	mucin-degrading	1161:1175	arg1	bacteria					1177:1184	the mucin-degrading bacteria	1157:1184	the mucin-degrading bacteria	1157:1184	Gut microbiome composition, including changes in the mucin-degrading bacteria, Akkermansia muciniphila and Ruminococcus gnavus, was altered significantly, as was expression of several genes affecting intestinal epithelial homeostasis in isolated colonoids, after C tropicalis infection compared with uninfected controls.
34890843	6	6	theme	mucin-degrading	1161:1175	arg1	muciniphila					1199:1209	Akkermansia muciniphila	1187:1209	Akkermansia muciniphila	1187:1209	Gut microbiome composition, including changes in the mucin-degrading bacteria, Akkermansia muciniphila and Ruminococcus gnavus, was altered significantly, as was expression of several genes affecting intestinal epithelial homeostasis in isolated colonoids, after C tropicalis infection compared with uninfected controls.
34890843	6	6	theme	mucin-degrading	1161:1175	arg1	gnavus					1228:1233	Ruminococcus gnavus	1215:1233	Ruminococcus gnavus	1215:1233	Gut microbiome composition, including changes in the mucin-degrading bacteria, Akkermansia muciniphila and Ruminococcus gnavus, was altered significantly, as was expression of several genes affecting intestinal epithelial homeostasis in isolated colonoids, after C tropicalis infection compared with uninfected controls.
34890843	8	7	theme	proinflammatory	1986:2000	arg1	phenotype					2002:2010	an accelerated proinflammatory phenotype	1971:2010	an accelerated proinflammatory phenotype in experimental colitic mice	1971:2039	CONCLUSIONS C tropicalis induces dysbiosis that involves changes in the presence of mucin-degrading bacteria, leading to altered tight junction protein expression with increased intestinal permeability and followed by induction of robust Th1/Th17 responses, which ultimately lead to an accelerated proinflammatory phenotype in experimental colitic mice.
34890843	1	8	theme	Candida	171:177	arg1	tropicalis					179:188	Candida tropicalis	171:188	Candida tropicalis	171:188	BACKGROUND & AIMS We previously showed that abundance of Candida tropicalis is significantly greater in Crohn's disease patients compared with first-degree relatives without Crohn's disease.
34890843	3	9	theme	sodium	563:568	arg1	sulfate					570:576	dextran sodium sulfate	555:576	dextran sodium sulfate	555:576	METHODS C57BL/6 mice were inoculated with C tropicalis, and colitis was induced by administration of dextran sodium sulfate in drinking water.
34890843	8	10	theme	tight	1817:1821	arg1	expression					1840:1849	altered tight junction protein expression	1809:1849	altered tight junction protein expression with increased intestinal permeability	1809:1888	CONCLUSIONS C tropicalis induces dysbiosis that involves changes in the presence of mucin-degrading bacteria, leading to altered tight junction protein expression with increased intestinal permeability and followed by induction of robust Th1/Th17 responses, which ultimately lead to an accelerated proinflammatory phenotype in experimental colitic mice.
34890843	4	11	dep	analyses	801:808	arg1	Technologies					822:833	NanoString Technologies	811:833	NanoString Technologies	811:833	Disease severity and intestinal permeability subsequently were evaluated by endoscopy, histology, quantitative reverse-transcription polymerase chain reaction, as well as 16S ribosomal RNA and NanoString analyses (NanoString Technologies, Seattle, WA).
34890843	4	11	dep	analyses	801:808	arg1	WA					845:846	WA	845:846	WA	845:846	Disease severity and intestinal permeability subsequently were evaluated by endoscopy, histology, quantitative reverse-transcription polymerase chain reaction, as well as 16S ribosomal RNA and NanoString analyses (NanoString Technologies, Seattle, WA).
34890843	8	12	with	expression	1840:1849	arg1	permeability					1877:1888	increased intestinal permeability	1856:1888	increased intestinal permeability	1856:1888	CONCLUSIONS C tropicalis induces dysbiosis that involves changes in the presence of mucin-degrading bacteria, leading to altered tight junction protein expression with increased intestinal permeability and followed by induction of robust Th1/Th17 responses, which ultimately lead to an accelerated proinflammatory phenotype in experimental colitic mice.
34890843	8	13	theme	colitic	2028:2034	arg1	mice					2036:2039	experimental colitic mice	2015:2039	experimental colitic mice	2015:2039	CONCLUSIONS C tropicalis induces dysbiosis that involves changes in the presence of mucin-degrading bacteria, leading to altered tight junction protein expression with increased intestinal permeability and followed by induction of robust Th1/Th17 responses, which ultimately lead to an accelerated proinflammatory phenotype in experimental colitic mice.
34890843	8	14	theme	protein	1832:1838	arg1	expression					1840:1849	altered tight junction protein expression	1809:1849	altered tight junction protein expression with increased intestinal permeability	1809:1888	CONCLUSIONS C tropicalis induces dysbiosis that involves changes in the presence of mucin-degrading bacteria, leading to altered tight junction protein expression with increased intestinal permeability and followed by induction of robust Th1/Th17 responses, which ultimately lead to an accelerated proinflammatory phenotype in experimental colitic mice.
34890843	1	15	theme	&	125:125	arg1	AIMS					127:130	BACKGROUND & AIMS	114:130	BACKGROUND & AIMS We	114:133	BACKGROUND & AIMS We previously showed that abundance of Candida tropicalis is significantly greater in Crohn's disease patients compared with first-degree relatives without Crohn's disease.
34890843	0	16	from	Susceptibility	79:92	arg1	Mice					108:111	Mice	108:111	Mice	108:111	Candida tropicalis Infection Modulates the Gut Microbiome and Confers Enhanced Susceptibility to Colitis in Mice.
34890843	5	17	theme	lymphoid	1058:1065	arg1	cells					1067:1071	mesenteric lymph node-derived group 2 innate lymphoid cells	1013:1071	mesenteric lymph node-derived group 2 innate lymphoid cells	1013:1071	RESULTS Infected mice showed more severe colitis, with alterations in gut mucosal helper T cells (Th)1 and Th17 cytokine expression, and an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells compared with uninfected controls.
34890843	5	18	theme	Infected	858:865	arg1	mice					867:870	Infected mice	858:870	Infected mice	858:870	RESULTS Infected mice showed more severe colitis, with alterations in gut mucosal helper T cells (Th)1 and Th17 cytokine expression, and an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells compared with uninfected controls.
34890843	4	19	theme	chain	741:745	arg1	reaction					747:754	quantitative reverse-transcription polymerase chain reaction	695:754	quantitative reverse-transcription polymerase chain reaction	695:754	Disease severity and intestinal permeability subsequently were evaluated by endoscopy, histology, quantitative reverse-transcription polymerase chain reaction, as well as 16S ribosomal RNA and NanoString analyses (NanoString Technologies, Seattle, WA).
34890843	6	20	theme	epithelial	1319:1328	arg1	homeostasis					1330:1340	intestinal epithelial homeostasis	1308:1340	intestinal epithelial homeostasis	1308:1340	Gut microbiome composition, including changes in the mucin-degrading bacteria, Akkermansia muciniphila and Ruminococcus gnavus, was altered significantly, as was expression of several genes affecting intestinal epithelial homeostasis in isolated colonoids, after C tropicalis infection compared with uninfected controls.
34890843	5	21	theme	mesenteric	1013:1022	arg1	cells					1067:1071	mesenteric lymph node-derived group 2 innate lymphoid cells	1013:1071	mesenteric lymph node-derived group 2 innate lymphoid cells	1013:1071	RESULTS Infected mice showed more severe colitis, with alterations in gut mucosal helper T cells (Th)1 and Th17 cytokine expression, and an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells compared with uninfected controls.
34890843	8	22	theme	intestinal	1866:1875	arg1	permeability					1877:1888	increased intestinal permeability	1856:1888	increased intestinal permeability	1856:1888	CONCLUSIONS C tropicalis induces dysbiosis that involves changes in the presence of mucin-degrading bacteria, leading to altered tight junction protein expression with increased intestinal permeability and followed by induction of robust Th1/Th17 responses, which ultimately lead to an accelerated proinflammatory phenotype in experimental colitic mice.
34890843	5	23	theme	gut	920:922	arg1	cells					941:945	gut mucosal helper T cells	920:945	gut mucosal helper T cells	920:945	RESULTS Infected mice showed more severe colitis, with alterations in gut mucosal helper T cells (Th)1 and Th17 cytokine expression, and an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells compared with uninfected controls.
34890843	5	24	link	node-derived	1030:1041	arg1	cells					1067:1071	mesenteric lymph node-derived group 2 innate lymphoid cells	1013:1071	mesenteric lymph node-derived group 2 innate lymphoid cells	1013:1071	RESULTS Infected mice showed more severe colitis, with alterations in gut mucosal helper T cells (Th)1 and Th17 cytokine expression, and an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells compared with uninfected controls.
34890843	5	25	theme	node-derived	1030:1041	arg1	cells					1067:1071	mesenteric lymph node-derived group 2 innate lymphoid cells	1013:1071	mesenteric lymph node-derived group 2 innate lymphoid cells	1013:1071	RESULTS Infected mice showed more severe colitis, with alterations in gut mucosal helper T cells (Th)1 and Th17 cytokine expression, and an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells compared with uninfected controls.
34890843	5	26	theme	helper	932:937	arg1	cells					941:945	gut mucosal helper T cells	920:945	gut mucosal helper T cells	920:945	RESULTS Infected mice showed more severe colitis, with alterations in gut mucosal helper T cells (Th)1 and Th17 cytokine expression, and an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells compared with uninfected controls.
34890843	3	27	theme	METHODS	454:460	arg1	mice					470:473	METHODS C57BL/6 mice	454:473	METHODS C57BL/6 mice	454:473	METHODS C57BL/6 mice were inoculated with C tropicalis, and colitis was induced by administration of dextran sodium sulfate in drinking water.
34890843	6	28	theme	uninfected	1408:1417	arg1	controls					1419:1426	uninfected controls	1408:1426	uninfected controls	1408:1426	Gut microbiome composition, including changes in the mucin-degrading bacteria, Akkermansia muciniphila and Ruminococcus gnavus, was altered significantly, as was expression of several genes affecting intestinal epithelial homeostasis in isolated colonoids, after C tropicalis infection compared with uninfected controls.
34890843	2	29	from	inflammation	421:432	arg1	mice					448:451	mice	448:451	mice	448:451	The aim of this study was to determine the effects and mechanisms of action of C tropicalis infection on intestinal inflammation and injury in mice.
34890843	4	30	theme	reverse-transcription	708:728	arg1	reaction					747:754	quantitative reverse-transcription polymerase chain reaction	695:754	quantitative reverse-transcription polymerase chain reaction	695:754	Disease severity and intestinal permeability subsequently were evaluated by endoscopy, histology, quantitative reverse-transcription polymerase chain reaction, as well as 16S ribosomal RNA and NanoString analyses (NanoString Technologies, Seattle, WA).
34890843	2	31	from	injury	438:443	arg1	mice					448:451	mice	448:451	mice	448:451	The aim of this study was to determine the effects and mechanisms of action of C tropicalis infection on intestinal inflammation and injury in mice.
34890843	5	32	with	colitis	891:897	arg1	Th					948:949	Th	948:949	Th	948:949	RESULTS Infected mice showed more severe colitis, with alterations in gut mucosal helper T cells (Th)1 and Th17 cytokine expression, and an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells compared with uninfected controls.
34890843	5	32	with	colitis	891:897	arg1	alterations					905:915	alterations	905:915	alterations in gut mucosal helper T cells (Th)1	905:951	RESULTS Infected mice showed more severe colitis, with alterations in gut mucosal helper T cells (Th)1 and Th17 cytokine expression, and an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells compared with uninfected controls.
34890843	5	32	with	colitis	891:897	arg1	expression					971:980	Th17 cytokine expression	957:980	Th17 cytokine expression	957:980	RESULTS Infected mice showed more severe colitis, with alterations in gut mucosal helper T cells (Th)1 and Th17 cytokine expression, and an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells compared with uninfected controls.
34890843	1	33	dep	AIMS	127:130	arg1	We					132:133	We	132:133	We	132:133	BACKGROUND & AIMS We previously showed that abundance of Candida tropicalis is significantly greater in Crohn's disease patients compared with first-degree relatives without Crohn's disease.
34890843	6	34	theme	tropicalis	1373:1382	arg1	infection					1384:1392	C tropicalis infection	1371:1392	C tropicalis infection compared with uninfected controls	1371:1426	Gut microbiome composition, including changes in the mucin-degrading bacteria, Akkermansia muciniphila and Ruminococcus gnavus, was altered significantly, as was expression of several genes affecting intestinal epithelial homeostasis in isolated colonoids, after C tropicalis infection compared with uninfected controls.
34890843	8	35	theme	Th1/Th17	1926:1933	arg1	responses					1935:1943	robust Th1/Th17 responses	1919:1943	robust Th1/Th17 responses	1919:1943	CONCLUSIONS C tropicalis induces dysbiosis that involves changes in the presence of mucin-degrading bacteria, leading to altered tight junction protein expression with increased intestinal permeability and followed by induction of robust Th1/Th17 responses, which ultimately lead to an accelerated proinflammatory phenotype in experimental colitic mice.
34890843	7	36	theme	proteins	1607:1614	arg1	expression					1570:1579	altered expression	1562:1579	altered expression of several tight-junction proteins	1562:1614	In line with these findings, fecal microbiome transplantation of germ-free recipient mice using infected vs uninfected donors showed altered expression of several tight-junction proteins and increased susceptibility to dextran sodium sulfate-induced colitis.
34890843	4	37	theme	NanoString	790:799	arg1	analyses					801:808	NanoString analyses	790:808	NanoString analyses (NanoString Technologies, Seattle, WA)	790:847	Disease severity and intestinal permeability subsequently were evaluated by endoscopy, histology, quantitative reverse-transcription polymerase chain reaction, as well as 16S ribosomal RNA and NanoString analyses (NanoString Technologies, Seattle, WA).
34890843	2	38	theme	C	384:384	arg1	infection					397:405	C tropicalis infection	384:405	C tropicalis infection	384:405	The aim of this study was to determine the effects and mechanisms of action of C tropicalis infection on intestinal inflammation and injury in mice.
34890843	5	39	theme	uninfected	1087:1096	arg1	controls					1098:1105	uninfected controls	1087:1105	uninfected controls	1087:1105	RESULTS Infected mice showed more severe colitis, with alterations in gut mucosal helper T cells (Th)1 and Th17 cytokine expression, and an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells compared with uninfected controls.
34890843	5	40	theme	Th17	957:960	arg1	expression					971:980	Th17 cytokine expression	957:980	Th17 cytokine expression	957:980	RESULTS Infected mice showed more severe colitis, with alterations in gut mucosal helper T cells (Th)1 and Th17 cytokine expression, and an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells compared with uninfected controls.
34890843	2	41	theme	action	374:379	arg1	effects					348:354	effects	348:354	effects	348:354	The aim of this study was to determine the effects and mechanisms of action of C tropicalis infection on intestinal inflammation and injury in mice.
34890843	2	41	theme	action	374:379	arg1	mechanisms					360:369	mechanisms	360:369	mechanisms	360:369	The aim of this study was to determine the effects and mechanisms of action of C tropicalis infection on intestinal inflammation and injury in mice.
34890843	0	42	theme	Candida	0:6	arg1	Infection					19:27	Candida tropicalis Infection	0:27	Candida tropicalis Infection	0:27	Candida tropicalis Infection Modulates the Gut Microbiome and Confers Enhanced Susceptibility to Colitis in Mice.
34890843	4	43	theme	16S	768:770	arg1	RNA					782:784	16S ribosomal RNA	768:784	16S ribosomal RNA	768:784	Disease severity and intestinal permeability subsequently were evaluated by endoscopy, histology, quantitative reverse-transcription polymerase chain reaction, as well as 16S ribosomal RNA and NanoString analyses (NanoString Technologies, Seattle, WA).
34890843	2	44	theme	intestinal	410:419	arg1	inflammation					421:432	intestinal inflammation	410:432	intestinal inflammation	410:432	The aim of this study was to determine the effects and mechanisms of action of C tropicalis infection on intestinal inflammation and injury in mice.
34890843	6	45	from	changes	1146:1152	arg1	bacteria					1177:1184	the mucin-degrading bacteria	1157:1184	the mucin-degrading bacteria	1157:1184	Gut microbiome composition, including changes in the mucin-degrading bacteria, Akkermansia muciniphila and Ruminococcus gnavus, was altered significantly, as was expression of several genes affecting intestinal epithelial homeostasis in isolated colonoids, after C tropicalis infection compared with uninfected controls.
34890843	6	45	from	changes	1146:1152	arg1	muciniphila					1199:1209	Akkermansia muciniphila	1187:1209	Akkermansia muciniphila	1187:1209	Gut microbiome composition, including changes in the mucin-degrading bacteria, Akkermansia muciniphila and Ruminococcus gnavus, was altered significantly, as was expression of several genes affecting intestinal epithelial homeostasis in isolated colonoids, after C tropicalis infection compared with uninfected controls.
34890843	6	45	from	changes	1146:1152	arg1	gnavus					1228:1233	Ruminococcus gnavus	1215:1233	Ruminococcus gnavus	1215:1233	Gut microbiome composition, including changes in the mucin-degrading bacteria, Akkermansia muciniphila and Ruminococcus gnavus, was altered significantly, as was expression of several genes affecting intestinal epithelial homeostasis in isolated colonoids, after C tropicalis infection compared with uninfected controls.
34890843	2	46	theme	infection	397:405	arg1	action					374:379	action	374:379	action of C tropicalis infection	374:405	The aim of this study was to determine the effects and mechanisms of action of C tropicalis infection on intestinal inflammation and injury in mice.
34890843	7	47	theme	altered	1562:1568	arg1	expression					1570:1579	altered expression	1562:1579	altered expression of several tight-junction proteins	1562:1614	In line with these findings, fecal microbiome transplantation of germ-free recipient mice using infected vs uninfected donors showed altered expression of several tight-junction proteins and increased susceptibility to dextran sodium sulfate-induced colitis.
34890843	5	48	theme	increased	990:998	arg1	frequency					1000:1008	an increased frequency	987:1008	an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells	987:1071	RESULTS Infected mice showed more severe colitis, with alterations in gut mucosal helper T cells (Th)1 and Th17 cytokine expression, and an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells compared with uninfected controls.
34890843	6	49	theme	microbiome	1112:1121	arg1	composition					1123:1133	Gut microbiome composition	1108:1133	Gut microbiome composition	1108:1133	Gut microbiome composition, including changes in the mucin-degrading bacteria, Akkermansia muciniphila and Ruminococcus gnavus, was altered significantly, as was expression of several genes affecting intestinal epithelial homeostasis in isolated colonoids, after C tropicalis infection compared with uninfected controls.
34890843	5	50	dep	RESULTS	850:856	arg1	showed					872:877	showed	872:877	showed more severe colitis, with alterations in gut mucosal helper T cells (Th)1 and Th17 cytokine expression, and an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells compared with uninfected controls	872:1105	RESULTS Infected mice showed more severe colitis, with alterations in gut mucosal helper T cells (Th)1 and Th17 cytokine expression, and an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells compared with uninfected controls.
34890843	6	51	theme	genes	1292:1296	arg1	expression					1270:1279	expression	1270:1279	expression of several genes affecting intestinal epithelial homeostasis in isolated colonoids	1270:1362	Gut microbiome composition, including changes in the mucin-degrading bacteria, Akkermansia muciniphila and Ruminococcus gnavus, was altered significantly, as was expression of several genes affecting intestinal epithelial homeostasis in isolated colonoids, after C tropicalis infection compared with uninfected controls.
34890843	3	52	theme	C	496:496	arg1	tropicalis					498:507	C tropicalis	496:507	C tropicalis	496:507	METHODS C57BL/6 mice were inoculated with C tropicalis, and colitis was induced by administration of dextran sodium sulfate in drinking water.
34890843	7	53	theme	recipient	1504:1512	arg1	mice					1514:1517	germ-free recipient mice	1494:1517	germ-free recipient mice using infected vs uninfected donors	1494:1553	In line with these findings, fecal microbiome transplantation of germ-free recipient mice using infected vs uninfected donors showed altered expression of several tight-junction proteins and increased susceptibility to dextran sodium sulfate-induced colitis.
34890843	8	54	theme	C	1700:1700	arg1	tropicalis					1702:1711	CONCLUSIONS C tropicalis	1688:1711	CONCLUSIONS C tropicalis	1688:1711	CONCLUSIONS C tropicalis induces dysbiosis that involves changes in the presence of mucin-degrading bacteria, leading to altered tight junction protein expression with increased intestinal permeability and followed by induction of robust Th1/Th17 responses, which ultimately lead to an accelerated proinflammatory phenotype in experimental colitic mice.
34890843	5	55	with	frequency	1000:1008	arg1	Th					948:949	Th	948:949	Th	948:949	RESULTS Infected mice showed more severe colitis, with alterations in gut mucosal helper T cells (Th)1 and Th17 cytokine expression, and an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells compared with uninfected controls.
34890843	5	55	with	frequency	1000:1008	arg1	alterations					905:915	alterations	905:915	alterations in gut mucosal helper T cells (Th)1	905:951	RESULTS Infected mice showed more severe colitis, with alterations in gut mucosal helper T cells (Th)1 and Th17 cytokine expression, and an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells compared with uninfected controls.
34890843	5	55	with	frequency	1000:1008	arg1	expression					971:980	Th17 cytokine expression	957:980	Th17 cytokine expression	957:980	RESULTS Infected mice showed more severe colitis, with alterations in gut mucosal helper T cells (Th)1 and Th17 cytokine expression, and an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells compared with uninfected controls.
34890843	8	56	theme	accelerated	1974:1984	arg1	phenotype					2002:2010	an accelerated proinflammatory phenotype	1971:2010	an accelerated proinflammatory phenotype in experimental colitic mice	1971:2039	CONCLUSIONS C tropicalis induces dysbiosis that involves changes in the presence of mucin-degrading bacteria, leading to altered tight junction protein expression with increased intestinal permeability and followed by induction of robust Th1/Th17 responses, which ultimately lead to an accelerated proinflammatory phenotype in experimental colitic mice.
34890843	6	57	dep	expression	1270:1279	arg1	as					1263:1264	as	1263:1264	as	1263:1264	Gut microbiome composition, including changes in the mucin-degrading bacteria, Akkermansia muciniphila and Ruminococcus gnavus, was altered significantly, as was expression of several genes affecting intestinal epithelial homeostasis in isolated colonoids, after C tropicalis infection compared with uninfected controls.
34890843	6	57	dep	expression	1270:1279	arg1	was					1266:1268	was	1266:1268	was	1266:1268	Gut microbiome composition, including changes in the mucin-degrading bacteria, Akkermansia muciniphila and Ruminococcus gnavus, was altered significantly, as was expression of several genes affecting intestinal epithelial homeostasis in isolated colonoids, after C tropicalis infection compared with uninfected controls.
34890843	7	58	theme	fecal	1458:1462	arg1	transplantation					1475:1489	fecal microbiome transplantation	1458:1489	fecal microbiome transplantation of germ-free recipient mice using infected vs uninfected donors	1458:1553	In line with these findings, fecal microbiome transplantation of germ-free recipient mice using infected vs uninfected donors showed altered expression of several tight-junction proteins and increased susceptibility to dextran sodium sulfate-induced colitis.
34890843	8	59	theme	bacteria	1788:1795	arg1	presence					1760:1767	the presence	1756:1767	the presence of mucin-degrading bacteria	1756:1795	CONCLUSIONS C tropicalis induces dysbiosis that involves changes in the presence of mucin-degrading bacteria, leading to altered tight junction protein expression with increased intestinal permeability and followed by induction of robust Th1/Th17 responses, which ultimately lead to an accelerated proinflammatory phenotype in experimental colitic mice.
34890843	7	60	theme	microbiome	1464:1473	arg1	transplantation					1475:1489	fecal microbiome transplantation	1458:1489	fecal microbiome transplantation of germ-free recipient mice using infected vs uninfected donors	1458:1553	In line with these findings, fecal microbiome transplantation of germ-free recipient mice using infected vs uninfected donors showed altered expression of several tight-junction proteins and increased susceptibility to dextran sodium sulfate-induced colitis.
34890843	2	61	from	effects	348:354	arg1	injury					438:443	injury	438:443	injury	438:443	The aim of this study was to determine the effects and mechanisms of action of C tropicalis infection on intestinal inflammation and injury in mice.
34890843	2	61	from	effects	348:354	arg1	inflammation					421:432	intestinal inflammation	410:432	intestinal inflammation	410:432	The aim of this study was to determine the effects and mechanisms of action of C tropicalis infection on intestinal inflammation and injury in mice.
34890843	8	62	theme	experimental	2015:2026	arg1	mice					2036:2039	experimental colitic mice	2015:2039	experimental colitic mice	2015:2039	CONCLUSIONS C tropicalis induces dysbiosis that involves changes in the presence of mucin-degrading bacteria, leading to altered tight junction protein expression with increased intestinal permeability and followed by induction of robust Th1/Th17 responses, which ultimately lead to an accelerated proinflammatory phenotype in experimental colitic mice.
34890843	3	63	theme	dextran	555:561	arg1	sulfate					570:576	dextran sodium sulfate	555:576	dextran sodium sulfate	555:576	METHODS C57BL/6 mice were inoculated with C tropicalis, and colitis was induced by administration of dextran sodium sulfate in drinking water.
34890843	5	64	from	expression	971:980	arg1	cells					941:945	gut mucosal helper T cells	920:945	gut mucosal helper T cells	920:945	RESULTS Infected mice showed more severe colitis, with alterations in gut mucosal helper T cells (Th)1 and Th17 cytokine expression, and an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells compared with uninfected controls.
34890843	1	65	theme	tropicalis	179:188	arg1	abundance					158:166	abundance	158:166	abundance of Candida tropicalis	158:188	BACKGROUND & AIMS We previously showed that abundance of Candida tropicalis is significantly greater in Crohn's disease patients compared with first-degree relatives without Crohn's disease.
34890843	2	66	from	mechanisms	360:369	arg1	injury					438:443	injury	438:443	injury	438:443	The aim of this study was to determine the effects and mechanisms of action of C tropicalis infection on intestinal inflammation and injury in mice.
34890843	2	66	from	mechanisms	360:369	arg1	inflammation					421:432	intestinal inflammation	410:432	intestinal inflammation	410:432	The aim of this study was to determine the effects and mechanisms of action of C tropicalis infection on intestinal inflammation and injury in mice.
34890843	8	67	theme	altered	1809:1815	arg1	expression					1840:1849	altered tight junction protein expression	1809:1849	altered tight junction protein expression with increased intestinal permeability	1809:1888	CONCLUSIONS C tropicalis induces dysbiosis that involves changes in the presence of mucin-degrading bacteria, leading to altered tight junction protein expression with increased intestinal permeability and followed by induction of robust Th1/Th17 responses, which ultimately lead to an accelerated proinflammatory phenotype in experimental colitic mice.
34890843	4	68	theme	intestinal	618:627	arg1	permeability					629:640	intestinal permeability	618:640	intestinal permeability	618:640	Disease severity and intestinal permeability subsequently were evaluated by endoscopy, histology, quantitative reverse-transcription polymerase chain reaction, as well as 16S ribosomal RNA and NanoString analyses (NanoString Technologies, Seattle, WA).
34890843	6	69	theme	isolated	1345:1352	arg1	colonoids					1354:1362	isolated colonoids	1345:1362	isolated colonoids	1345:1362	Gut microbiome composition, including changes in the mucin-degrading bacteria, Akkermansia muciniphila and Ruminococcus gnavus, was altered significantly, as was expression of several genes affecting intestinal epithelial homeostasis in isolated colonoids, after C tropicalis infection compared with uninfected controls.
34890843	5	70	theme	innate	1051:1056	arg1	cells					1067:1071	mesenteric lymph node-derived group 2 innate lymphoid cells	1013:1071	mesenteric lymph node-derived group 2 innate lymphoid cells	1013:1071	RESULTS Infected mice showed more severe colitis, with alterations in gut mucosal helper T cells (Th)1 and Th17 cytokine expression, and an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells compared with uninfected controls.
34890843	5	71	theme	severe	884:889	arg1	colitis					891:897	more severe colitis	879:897	more severe colitis	879:897	RESULTS Infected mice showed more severe colitis, with alterations in gut mucosal helper T cells (Th)1 and Th17 cytokine expression, and an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells compared with uninfected controls.
34890843	5	72	theme	cells	1067:1071	arg1	colitis					891:897	more severe colitis	879:897	more severe colitis	879:897	RESULTS Infected mice showed more severe colitis, with alterations in gut mucosal helper T cells (Th)1 and Th17 cytokine expression, and an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells compared with uninfected controls.
34890843	5	72	theme	cells	1067:1071	arg1	frequency					1000:1008	an increased frequency	987:1008	an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells	987:1071	RESULTS Infected mice showed more severe colitis, with alterations in gut mucosal helper T cells (Th)1 and Th17 cytokine expression, and an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells compared with uninfected controls.
34890843	3	73	theme	sulfate	570:576	arg1	administration					537:550	administration	537:550	administration of dextran sodium sulfate	537:576	METHODS C57BL/6 mice were inoculated with C tropicalis, and colitis was induced by administration of dextran sodium sulfate in drinking water.
34890843	1	74	theme	BACKGROUND	114:123	arg1	AIMS					127:130	BACKGROUND & AIMS	114:130	BACKGROUND & AIMS We	114:133	BACKGROUND & AIMS We previously showed that abundance of Candida tropicalis is significantly greater in Crohn's disease patients compared with first-degree relatives without Crohn's disease.
34890843	7	75	theme	sulfate-induced	1663:1677	arg1	colitis					1679:1685	dextran sodium sulfate-induced colitis	1648:1685	dextran sodium sulfate-induced colitis	1648:1685	In line with these findings, fecal microbiome transplantation of germ-free recipient mice using infected vs uninfected donors showed altered expression of several tight-junction proteins and increased susceptibility to dextran sodium sulfate-induced colitis.
34890843	4	76	theme	polymerase	730:739	arg1	reaction					747:754	quantitative reverse-transcription polymerase chain reaction	695:754	quantitative reverse-transcription polymerase chain reaction	695:754	Disease severity and intestinal permeability subsequently were evaluated by endoscopy, histology, quantitative reverse-transcription polymerase chain reaction, as well as 16S ribosomal RNA and NanoString analyses (NanoString Technologies, Seattle, WA).
34890843	6	77	theme	intestinal	1308:1317	arg1	homeostasis					1330:1340	intestinal epithelial homeostasis	1308:1340	intestinal epithelial homeostasis	1308:1340	Gut microbiome composition, including changes in the mucin-degrading bacteria, Akkermansia muciniphila and Ruminococcus gnavus, was altered significantly, as was expression of several genes affecting intestinal epithelial homeostasis in isolated colonoids, after C tropicalis infection compared with uninfected controls.
34890843	5	78	theme	lymph	1024:1028	arg1	cells					1067:1071	mesenteric lymph node-derived group 2 innate lymphoid cells	1013:1071	mesenteric lymph node-derived group 2 innate lymphoid cells	1013:1071	RESULTS Infected mice showed more severe colitis, with alterations in gut mucosal helper T cells (Th)1 and Th17 cytokine expression, and an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells compared with uninfected controls.
34890843	8	79	theme	increased	1856:1864	arg1	permeability					1877:1888	increased intestinal permeability	1856:1888	increased intestinal permeability	1856:1888	CONCLUSIONS C tropicalis induces dysbiosis that involves changes in the presence of mucin-degrading bacteria, leading to altered tight junction protein expression with increased intestinal permeability and followed by induction of robust Th1/Th17 responses, which ultimately lead to an accelerated proinflammatory phenotype in experimental colitic mice.
34890843	5	80	theme	mucosal	924:930	arg1	cells					941:945	gut mucosal helper T cells	920:945	gut mucosal helper T cells	920:945	RESULTS Infected mice showed more severe colitis, with alterations in gut mucosal helper T cells (Th)1 and Th17 cytokine expression, and an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells compared with uninfected controls.
34890843	2	81	dep	effects	348:354	arg1	the					344:346	the	344:346	the	344:346	The aim of this study was to determine the effects and mechanisms of action of C tropicalis infection on intestinal inflammation and injury in mice.
34890843	0	82	theme	Enhanced	70:77	arg1	Susceptibility					79:92	Enhanced Susceptibility	70:92	Enhanced Susceptibility to Colitis in Mice	70:111	Candida tropicalis Infection Modulates the Gut Microbiome and Confers Enhanced Susceptibility to Colitis in Mice.
34890843	5	83	theme	group	1043:1047	arg1	cells					1067:1071	mesenteric lymph node-derived group 2 innate lymphoid cells	1013:1071	mesenteric lymph node-derived group 2 innate lymphoid cells	1013:1071	RESULTS Infected mice showed more severe colitis, with alterations in gut mucosal helper T cells (Th)1 and Th17 cytokine expression, and an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells compared with uninfected controls.
34890843	8	84	dep	induces	1713:1719	arg1	leading					1798:1804	leading	1798:1804	leading to altered tight junction protein expression with increased intestinal permeability	1798:1888	CONCLUSIONS C tropicalis induces dysbiosis that involves changes in the presence of mucin-degrading bacteria, leading to altered tight junction protein expression with increased intestinal permeability and followed by induction of robust Th1/Th17 responses, which ultimately lead to an accelerated proinflammatory phenotype in experimental colitic mice.
34890843	8	84	dep	induces	1713:1719	arg1	followed					1894:1901	followed	1894:1901	followed by induction of robust Th1/Th17 responses, which ultimately lead to an accelerated proinflammatory phenotype in experimental colitic mice	1894:2039	CONCLUSIONS C tropicalis induces dysbiosis that involves changes in the presence of mucin-degrading bacteria, leading to altered tight junction protein expression with increased intestinal permeability and followed by induction of robust Th1/Th17 responses, which ultimately lead to an accelerated proinflammatory phenotype in experimental colitic mice.
34890843	4	85	theme	quantitative	695:706	arg1	reaction					747:754	quantitative reverse-transcription polymerase chain reaction	695:754	quantitative reverse-transcription polymerase chain reaction	695:754	Disease severity and intestinal permeability subsequently were evaluated by endoscopy, histology, quantitative reverse-transcription polymerase chain reaction, as well as 16S ribosomal RNA and NanoString analyses (NanoString Technologies, Seattle, WA).
34890843	5	86	from	alterations	905:915	arg1	cells					941:945	gut mucosal helper T cells	920:945	gut mucosal helper T cells	920:945	RESULTS Infected mice showed more severe colitis, with alterations in gut mucosal helper T cells (Th)1 and Th17 cytokine expression, and an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells compared with uninfected controls.
34890843	7	87	theme	tight-junction	1592:1605	arg1	proteins					1607:1614	several tight-junction proteins	1584:1614	several tight-junction proteins	1584:1614	In line with these findings, fecal microbiome transplantation of germ-free recipient mice using infected vs uninfected donors showed altered expression of several tight-junction proteins and increased susceptibility to dextran sodium sulfate-induced colitis.
34890843	8	88	from	changes	1745:1751	arg1	presence					1760:1767	the presence	1756:1767	the presence of mucin-degrading bacteria	1756:1795	CONCLUSIONS C tropicalis induces dysbiosis that involves changes in the presence of mucin-degrading bacteria, leading to altered tight junction protein expression with increased intestinal permeability and followed by induction of robust Th1/Th17 responses, which ultimately lead to an accelerated proinflammatory phenotype in experimental colitic mice.
34890843	4	89	theme	ribosomal	772:780	arg1	RNA					782:784	16S ribosomal RNA	768:784	16S ribosomal RNA	768:784	Disease severity and intestinal permeability subsequently were evaluated by endoscopy, histology, quantitative reverse-transcription polymerase chain reaction, as well as 16S ribosomal RNA and NanoString analyses (NanoString Technologies, Seattle, WA).
34890843	6	90	theme	C	1371:1371	arg1	infection					1384:1392	C tropicalis infection	1371:1392	C tropicalis infection compared with uninfected controls	1371:1426	Gut microbiome composition, including changes in the mucin-degrading bacteria, Akkermansia muciniphila and Ruminococcus gnavus, was altered significantly, as was expression of several genes affecting intestinal epithelial homeostasis in isolated colonoids, after C tropicalis infection compared with uninfected controls.
34890843	8	91	theme	robust	1919:1924	arg1	responses					1935:1943	robust Th1/Th17 responses	1919:1943	robust Th1/Th17 responses	1919:1943	CONCLUSIONS C tropicalis induces dysbiosis that involves changes in the presence of mucin-degrading bacteria, leading to altered tight junction protein expression with increased intestinal permeability and followed by induction of robust Th1/Th17 responses, which ultimately lead to an accelerated proinflammatory phenotype in experimental colitic mice.
34890843	1	92	theme	disease	226:232	arg1	patients					234:241	Crohn's disease patients	218:241	Crohn's disease patients	218:241	BACKGROUND & AIMS We previously showed that abundance of Candida tropicalis is significantly greater in Crohn's disease patients compared with first-degree relatives without Crohn's disease.
34890843	0	93	theme	tropicalis	8:17	arg1	Infection					19:27	Candida tropicalis Infection	0:27	Candida tropicalis Infection	0:27	Candida tropicalis Infection Modulates the Gut Microbiome and Confers Enhanced Susceptibility to Colitis in Mice.
34890843	8	94	theme	responses	1935:1943	arg1	induction					1906:1914	induction	1906:1914	induction	1906:1914	CONCLUSIONS C tropicalis induces dysbiosis that involves changes in the presence of mucin-degrading bacteria, leading to altered tight junction protein expression with increased intestinal permeability and followed by induction of robust Th1/Th17 responses, which ultimately lead to an accelerated proinflammatory phenotype in experimental colitic mice.
34890843	5	95	theme	T	939:939	arg1	cells					941:945	gut mucosal helper T cells	920:945	gut mucosal helper T cells	920:945	RESULTS Infected mice showed more severe colitis, with alterations in gut mucosal helper T cells (Th)1 and Th17 cytokine expression, and an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells compared with uninfected controls.
34890843	7	96	with	line	1432:1435	arg1	findings					1448:1455	these findings	1442:1455	these findings	1442:1455	In line with these findings, fecal microbiome transplantation of germ-free recipient mice using infected vs uninfected donors showed altered expression of several tight-junction proteins and increased susceptibility to dextran sodium sulfate-induced colitis.
34890843	8	97	theme	junction	1823:1830	arg1	expression					1840:1849	altered tight junction protein expression	1809:1849	altered tight junction protein expression with increased intestinal permeability	1809:1888	CONCLUSIONS C tropicalis induces dysbiosis that involves changes in the presence of mucin-degrading bacteria, leading to altered tight junction protein expression with increased intestinal permeability and followed by induction of robust Th1/Th17 responses, which ultimately lead to an accelerated proinflammatory phenotype in experimental colitic mice.
34890843	3	98	theme	C57BL/6	462:468	arg1	mice					470:473	METHODS C57BL/6 mice	454:473	METHODS C57BL/6 mice	454:473	METHODS C57BL/6 mice were inoculated with C tropicalis, and colitis was induced by administration of dextran sodium sulfate in drinking water.
34890843	0	99	theme	Gut	43:45	arg1	Microbiome					47:56	the Gut Microbiome	39:56	the Gut Microbiome	39:56	Candida tropicalis Infection Modulates the Gut Microbiome and Confers Enhanced Susceptibility to Colitis in Mice.
34890843	7	100	theme	several	1584:1590	arg1	proteins					1607:1614	several tight-junction proteins	1584:1614	several tight-junction proteins	1584:1614	In line with these findings, fecal microbiome transplantation of germ-free recipient mice using infected vs uninfected donors showed altered expression of several tight-junction proteins and increased susceptibility to dextran sodium sulfate-induced colitis.
34890843	2	101	theme	tropicalis	386:395	arg1	infection					397:405	C tropicalis infection	384:405	C tropicalis infection	384:405	The aim of this study was to determine the effects and mechanisms of action of C tropicalis infection on intestinal inflammation and injury in mice.
34890843	8	102	from	phenotype	2002:2010	arg1	mice					2036:2039	experimental colitic mice	2015:2039	experimental colitic mice	2015:2039	CONCLUSIONS C tropicalis induces dysbiosis that involves changes in the presence of mucin-degrading bacteria, leading to altered tight junction protein expression with increased intestinal permeability and followed by induction of robust Th1/Th17 responses, which ultimately lead to an accelerated proinflammatory phenotype in experimental colitic mice.
34890843	5	103	theme	cytokine	962:969	arg1	expression					971:980	Th17 cytokine expression	957:980	Th17 cytokine expression	957:980	RESULTS Infected mice showed more severe colitis, with alterations in gut mucosal helper T cells (Th)1 and Th17 cytokine expression, and an increased frequency of mesenteric lymph node-derived group 2 innate lymphoid cells compared with uninfected controls.
34890843	6	104	theme	Gut	1108:1110	arg1	composition					1123:1133	Gut microbiome composition	1108:1133	Gut microbiome composition	1108:1133	Gut microbiome composition, including changes in the mucin-degrading bacteria, Akkermansia muciniphila and Ruminococcus gnavus, was altered significantly, as was expression of several genes affecting intestinal epithelial homeostasis in isolated colonoids, after C tropicalis infection compared with uninfected controls.
34890843	4	105	theme	Disease	597:603	arg1	severity					605:612	Disease severity	597:612	Disease severity	597:612	Disease severity and intestinal permeability subsequently were evaluated by endoscopy, histology, quantitative reverse-transcription polymerase chain reaction, as well as 16S ribosomal RNA and NanoString analyses (NanoString Technologies, Seattle, WA).
34890843	2	106	theme	study	321:325	arg1	aim					309:311	The aim	305:311	The aim of this study	305:325	The aim of this study was to determine the effects and mechanisms of action of C tropicalis infection on intestinal inflammation and injury in mice.
34890843	6	107	from	expression	1270:1279	arg1	colonoids					1354:1362	isolated colonoids	1345:1362	isolated colonoids	1345:1362	Gut microbiome composition, including changes in the mucin-degrading bacteria, Akkermansia muciniphila and Ruminococcus gnavus, was altered significantly, as was expression of several genes affecting intestinal epithelial homeostasis in isolated colonoids, after C tropicalis infection compared with uninfected controls.
34890843	7	108	theme	uninfected	1537:1546	arg1	donors					1548:1553	infected vs uninfected donors	1525:1553	infected vs uninfected donors	1525:1553	In line with these findings, fecal microbiome transplantation of germ-free recipient mice using infected vs uninfected donors showed altered expression of several tight-junction proteins and increased susceptibility to dextran sodium sulfate-induced colitis.
34890843	8	109	theme	CONCLUSIONS	1688:1698	arg1	tropicalis					1702:1711	CONCLUSIONS C tropicalis	1688:1711	CONCLUSIONS C tropicalis	1688:1711	CONCLUSIONS C tropicalis induces dysbiosis that involves changes in the presence of mucin-degrading bacteria, leading to altered tight junction protein expression with increased intestinal permeability and followed by induction of robust Th1/Th17 responses, which ultimately lead to an accelerated proinflammatory phenotype in experimental colitic mice.
34890843	4	110	theme	NanoString	811:820	arg1	Technologies					822:833	NanoString Technologies	811:833	NanoString Technologies	811:833	Disease severity and intestinal permeability subsequently were evaluated by endoscopy, histology, quantitative reverse-transcription polymerase chain reaction, as well as 16S ribosomal RNA and NanoString analyses (NanoString Technologies, Seattle, WA).
34890843	4	110	theme	NanoString	811:820	arg1	WA					845:846	WA	845:846	WA	845:846	Disease severity and intestinal permeability subsequently were evaluated by endoscopy, histology, quantitative reverse-transcription polymerase chain reaction, as well as 16S ribosomal RNA and NanoString analyses (NanoString Technologies, Seattle, WA).
32724972	6	0	theme	intercellular	1205:1217	arg1	interaction					1219:1229	the intercellular interaction	1201:1229	the intercellular interaction between MSCs and CMs	1201:1250	The in vitro cell experiments demonstrated that the BG/γ-PGA/CS hydrogel activated the intercellular interaction between MSCs and CMs, which resulted in reduced cell apoptosis and enhanced angiogenesis.
32724972	4	1	theme	infarction	601:610	arg1	model					618:622	a rat acute myocardial infarction (AMI) model	578:622	a rat acute myocardial infarction (AMI) model	578:622	And the effect of the composite hydrogel on MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo and the possible mechanism of the BG/γ-PGA/CS hydrogel for the stimulation of the intercellular interaction between MSCs and cardiomyocytes (CMs) was explored by a MSC and CM co-culture experiment in vitro.
32724972	7	2	theme	cardiac	1401:1407	arg1	tissue					1409:1414	cardiac tissue	1401:1414	cardiac tissue after AMI	1401:1424	Silicate based bioactive hydrogels activated MSCs and cell-cell interactions in cardiac tissue after AMI and significantly enhanced the efficacy, which suggests that this bioactive hydrogel based approach is an effective way to enhance stem-cell therapy.
32724972	5	3	theme	accelerated	1089:1099	arg1	vascularization					1101:1115	accelerated vascularization	1089:1115	accelerated vascularization	1089:1115	The implantation of the MSC loaded BG/γ-PGA/CS composite hydrogel in the mice AMI model showed a significant improvement in the therapeutic efficacy with improved cardiac function, attenuation of heart remodeling, reduced cardiomyocyte apoptosis and accelerated vascularization.
32724972	0	4	theme	bioactive	51:59	arg1	hydrogels					61:69	bioactive hydrogels	51:69	bioactive hydrogels for myocardial infarction treatment	51:105	In situ activated mesenchymal stem cells (MSCs) by bioactive hydrogels for myocardial infarction treatment.
32724972	4	5	theme	myocardial	590:599	arg1	AMI					613:615	AMI	613:615	AMI	613:615	And the effect of the composite hydrogel on MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo and the possible mechanism of the BG/γ-PGA/CS hydrogel for the stimulation of the intercellular interaction between MSCs and cardiomyocytes (CMs) was explored by a MSC and CM co-culture experiment in vitro.
32724972	4	5	theme	myocardial	590:599	arg1	infarction					601:610	a rat acute myocardial infarction	578:610	a rat acute myocardial infarction (AMI) model	578:622	And the effect of the composite hydrogel on MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo and the possible mechanism of the BG/γ-PGA/CS hydrogel for the stimulation of the intercellular interaction between MSCs and cardiomyocytes (CMs) was explored by a MSC and CM co-culture experiment in vitro.
32724972	5	6	theme	significant	936:946	arg1	improvement					948:958	a significant improvement	934:958	a significant improvement in the therapeutic efficacy with improved cardiac function, attenuation of heart remodeling, reduced cardiomyocyte apoptosis and accelerated vascularization	934:1115	The implantation of the MSC loaded BG/γ-PGA/CS composite hydrogel in the mice AMI model showed a significant improvement in the therapeutic efficacy with improved cardiac function, attenuation of heart remodeling, reduced cardiomyocyte apoptosis and accelerated vascularization.
32724972	0	7	theme	myocardial	75:84	arg1	infarction					86:95	myocardial infarction	75:95	myocardial infarction treatment	75:105	In situ activated mesenchymal stem cells (MSCs) by bioactive hydrogels for myocardial infarction treatment.
32724972	5	8	theme	loaded	867:872	arg1	hydrogel					896:903	the MSC loaded BG/γ-PGA/CS composite hydrogel	859:903	the MSC loaded BG/γ-PGA/CS composite hydrogel	859:903	The implantation of the MSC loaded BG/γ-PGA/CS composite hydrogel in the mice AMI model showed a significant improvement in the therapeutic efficacy with improved cardiac function, attenuation of heart remodeling, reduced cardiomyocyte apoptosis and accelerated vascularization.
32724972	5	9	theme	cardiomyocyte	1061:1073	arg1	apoptosis					1075:1083	reduced cardiomyocyte apoptosis	1053:1083	reduced cardiomyocyte apoptosis	1053:1083	The implantation of the MSC loaded BG/γ-PGA/CS composite hydrogel in the mice AMI model showed a significant improvement in the therapeutic efficacy with improved cardiac function, attenuation of heart remodeling, reduced cardiomyocyte apoptosis and accelerated vascularization.
32724972	3	10	theme	imine	471:475	arg1	formation					482:490	the imine bond formation	467:490	the imine bond formation	467:490	In this study, a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel was obtained by in situ adding BG to stimulate the imine bond formation.
32724972	7	11	theme	bioactive	1492:1500	arg1	approach					1517:1524	this bioactive hydrogel based approach	1487:1524	this bioactive hydrogel based approach	1487:1524	Silicate based bioactive hydrogels activated MSCs and cell-cell interactions in cardiac tissue after AMI and significantly enhanced the efficacy, which suggests that this bioactive hydrogel based approach is an effective way to enhance stem-cell therapy.
32724972	7	11	theme	bioactive	1492:1500	arg1	way					1542:1544	an effective way	1529:1544	an effective way to enhance stem-cell therapy	1529:1573	Silicate based bioactive hydrogels activated MSCs and cell-cell interactions in cardiac tissue after AMI and significantly enhanced the efficacy, which suggests that this bioactive hydrogel based approach is an effective way to enhance stem-cell therapy.
32724972	3	12	theme	bond	477:480	arg1	formation					482:490	the imine bond formation	467:490	the imine bond formation	467:490	In this study, a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel was obtained by in situ adding BG to stimulate the imine bond formation.
32724972	5	13	theme	therapeutic	967:977	arg1	efficacy					979:986	the therapeutic efficacy	963:986	the therapeutic efficacy with improved cardiac function, attenuation of heart remodeling, reduced cardiomyocyte apoptosis and accelerated vascularization	963:1115	The implantation of the MSC loaded BG/γ-PGA/CS composite hydrogel in the mice AMI model showed a significant improvement in the therapeutic efficacy with improved cardiac function, attenuation of heart remodeling, reduced cardiomyocyte apoptosis and accelerated vascularization.
32724972	7	14	theme	cell-cell	1375:1383	arg1	interactions					1385:1396	cell-cell interactions	1375:1396	cell-cell interactions	1375:1396	Silicate based bioactive hydrogels activated MSCs and cell-cell interactions in cardiac tissue after AMI and significantly enhanced the efficacy, which suggests that this bioactive hydrogel based approach is an effective way to enhance stem-cell therapy.
32724972	4	15	from	mechanism	649:657	arg1	efficacy					552:559	MI therapeutic efficacy	537:559	MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo	537:630	And the effect of the composite hydrogel on MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo and the possible mechanism of the BG/γ-PGA/CS hydrogel for the stimulation of the intercellular interaction between MSCs and cardiomyocytes (CMs) was explored by a MSC and CM co-culture experiment in vitro.
32724972	4	16	theme	possible	640:647	arg1	mechanism					649:657	the possible mechanism	636:657	the possible mechanism of the BG/γ-PGA/CS hydrogel for the stimulation of the intercellular interaction between MSCs and cardiomyocytes (CMs)	636:776	And the effect of the composite hydrogel on MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo and the possible mechanism of the BG/γ-PGA/CS hydrogel for the stimulation of the intercellular interaction between MSCs and cardiomyocytes (CMs) was explored by a MSC and CM co-culture experiment in vitro.
32724972	4	17	from	experiment	818:827	arg1	vitro					832:836	vitro	832:836	vitro	832:836	And the effect of the composite hydrogel on MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo and the possible mechanism of the BG/γ-PGA/CS hydrogel for the stimulation of the intercellular interaction between MSCs and cardiomyocytes (CMs) was explored by a MSC and CM co-culture experiment in vitro.
32724972	6	18	theme	reduced	1271:1277	arg1	apoptosis					1284:1292	reduced cell apoptosis	1271:1292	reduced cell apoptosis	1271:1292	The in vitro cell experiments demonstrated that the BG/γ-PGA/CS hydrogel activated the intercellular interaction between MSCs and CMs, which resulted in reduced cell apoptosis and enhanced angiogenesis.
32724972	6	19	theme	BG/γ-PGA/CS	1170:1180	arg1	hydrogel					1182:1189	the BG/γ-PGA/CS hydrogel	1166:1189	the BG/γ-PGA/CS hydrogel	1166:1189	The in vitro cell experiments demonstrated that the BG/γ-PGA/CS hydrogel activated the intercellular interaction between MSCs and CMs, which resulted in reduced cell apoptosis and enhanced angiogenesis.
32724972	7	20	theme	hydrogel	1502:1509	arg1	approach					1517:1524	this bioactive hydrogel based approach	1487:1524	this bioactive hydrogel based approach	1487:1524	Silicate based bioactive hydrogels activated MSCs and cell-cell interactions in cardiac tissue after AMI and significantly enhanced the efficacy, which suggests that this bioactive hydrogel based approach is an effective way to enhance stem-cell therapy.
32724972	7	20	theme	hydrogel	1502:1509	arg1	way					1542:1544	an effective way	1529:1544	an effective way to enhance stem-cell therapy	1529:1573	Silicate based bioactive hydrogels activated MSCs and cell-cell interactions in cardiac tissue after AMI and significantly enhanced the efficacy, which suggests that this bioactive hydrogel based approach is an effective way to enhance stem-cell therapy.
32724972	3	21	theme	adding	444:449	arg1	BG					451:452	in situ adding BG to stimulate the imine bond formation	436:490	in situ adding BG to stimulate the imine bond formation	436:490	In this study, a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel was obtained by in situ adding BG to stimulate the imine bond formation.
32724972	1	22	theme	promising	147:155	arg1	strategy					157:164	a promising strategy	145:164	a promising strategy for myocardial infarction (MI) treatment	145:205	Stem-cell therapy has been proved as a promising strategy for myocardial infarction (MI) treatment.
32724972	0	23	theme	infarction	86:95	arg1	treatment					97:105	myocardial infarction treatment	75:105	myocardial infarction treatment	75:105	In situ activated mesenchymal stem cells (MSCs) by bioactive hydrogels for myocardial infarction treatment.
32724972	4	24	theme	interaction	728:738	arg1	stimulation					695:705	the stimulation	691:705	the stimulation of the intercellular interaction between MSCs and cardiomyocytes (CMs)	691:776	And the effect of the composite hydrogel on MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo and the possible mechanism of the BG/γ-PGA/CS hydrogel for the stimulation of the intercellular interaction between MSCs and cardiomyocytes (CMs) was explored by a MSC and CM co-culture experiment in vitro.
32724972	7	25	theme	based	1511:1515	arg1	approach					1517:1524	this bioactive hydrogel based approach	1487:1524	this bioactive hydrogel based approach	1487:1524	Silicate based bioactive hydrogels activated MSCs and cell-cell interactions in cardiac tissue after AMI and significantly enhanced the efficacy, which suggests that this bioactive hydrogel based approach is an effective way to enhance stem-cell therapy.
32724972	7	25	theme	based	1511:1515	arg1	way					1542:1544	an effective way	1529:1544	an effective way to enhance stem-cell therapy	1529:1573	Silicate based bioactive hydrogels activated MSCs and cell-cell interactions in cardiac tissue after AMI and significantly enhanced the efficacy, which suggests that this bioactive hydrogel based approach is an effective way to enhance stem-cell therapy.
32724972	6	26	dep	in	1122:1123	arg1	vitro					1125:1129	vitro	1125:1129	vitro	1125:1129	The in vitro cell experiments demonstrated that the BG/γ-PGA/CS hydrogel activated the intercellular interaction between MSCs and CMs, which resulted in reduced cell apoptosis and enhanced angiogenesis.
32724972	5	27	theme	improved	993:1000	arg1	function					1010:1017	improved cardiac function	993:1017	improved cardiac function	993:1017	The implantation of the MSC loaded BG/γ-PGA/CS composite hydrogel in the mice AMI model showed a significant improvement in the therapeutic efficacy with improved cardiac function, attenuation of heart remodeling, reduced cardiomyocyte apoptosis and accelerated vascularization.
32724972	2	28	theme	transplanted	288:299	arg1	cells					318:322	transplanted mesenchymal stem cells	288:322	transplanted mesenchymal stem cells (MSCs)	288:329	However, the therapeutic efficacy is mainly limited by the cellular activity of transplanted mesenchymal stem cells (MSCs).
32724972	2	28	theme	transplanted	288:299	arg1	MSCs					325:328	MSCs	325:328	MSCs	325:328	However, the therapeutic efficacy is mainly limited by the cellular activity of transplanted mesenchymal stem cells (MSCs).
32724972	5	29	theme	reduced	1053:1059	arg1	apoptosis					1075:1083	reduced cardiomyocyte apoptosis	1053:1083	reduced cardiomyocyte apoptosis	1053:1083	The implantation of the MSC loaded BG/γ-PGA/CS composite hydrogel in the mice AMI model showed a significant improvement in the therapeutic efficacy with improved cardiac function, attenuation of heart remodeling, reduced cardiomyocyte apoptosis and accelerated vascularization.
32724972	1	30	theme	myocardial	170:179	arg1	MI					193:194	MI	193:194	MI	193:194	Stem-cell therapy has been proved as a promising strategy for myocardial infarction (MI) treatment.
32724972	1	30	theme	myocardial	170:179	arg1	infarction					181:190	myocardial infarction	170:190	myocardial infarction (MI) treatment	170:205	Stem-cell therapy has been proved as a promising strategy for myocardial infarction (MI) treatment.
32724972	0	31	theme	mesenchymal	18:28	arg1	cells					35:39	mesenchymal stem cells	18:39	mesenchymal stem cells (MSCs)	18:46	In situ activated mesenchymal stem cells (MSCs) by bioactive hydrogels for myocardial infarction treatment.
32724972	0	31	theme	mesenchymal	18:28	arg1	MSCs					42:45	MSCs	42:45	MSCs	42:45	In situ activated mesenchymal stem cells (MSCs) by bioactive hydrogels for myocardial infarction treatment.
32724972	5	32	theme	BG/γ-PGA/CS	874:884	arg1	hydrogel					896:903	the MSC loaded BG/γ-PGA/CS composite hydrogel	859:903	the MSC loaded BG/γ-PGA/CS composite hydrogel	859:903	The implantation of the MSC loaded BG/γ-PGA/CS composite hydrogel in the mice AMI model showed a significant improvement in the therapeutic efficacy with improved cardiac function, attenuation of heart remodeling, reduced cardiomyocyte apoptosis and accelerated vascularization.
32724972	6	33	dep	activated	1191:1199	arg1	enhanced					1298:1305	enhanced	1298:1305	enhanced angiogenesis	1298:1318	The in vitro cell experiments demonstrated that the BG/γ-PGA/CS hydrogel activated the intercellular interaction between MSCs and CMs, which resulted in reduced cell apoptosis and enhanced angiogenesis.
32724972	6	33	dep	activated	1191:1199	arg1	resulted					1259:1266	resulted	1259:1266	resulted in reduced cell apoptosis	1259:1292	The in vitro cell experiments demonstrated that the BG/γ-PGA/CS hydrogel activated the intercellular interaction between MSCs and CMs, which resulted in reduced cell apoptosis and enhanced angiogenesis.
32724972	6	34	theme	in	1122:1123	arg1	experiments					1136:1146	The in vitro cell experiments	1118:1146	The in vitro cell experiments	1118:1146	The in vitro cell experiments demonstrated that the BG/γ-PGA/CS hydrogel activated the intercellular interaction between MSCs and CMs, which resulted in reduced cell apoptosis and enhanced angiogenesis.
32724972	3	35	dep	in	436:437	arg1	situ					439:442	situ	439:442	situ	439:442	In this study, a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel was obtained by in situ adding BG to stimulate the imine bond formation.
32724972	7	36	theme	effective	1532:1540	arg1	approach					1517:1524	this bioactive hydrogel based approach	1487:1524	this bioactive hydrogel based approach	1487:1524	Silicate based bioactive hydrogels activated MSCs and cell-cell interactions in cardiac tissue after AMI and significantly enhanced the efficacy, which suggests that this bioactive hydrogel based approach is an effective way to enhance stem-cell therapy.
32724972	7	36	theme	effective	1532:1540	arg1	way					1542:1544	an effective way	1529:1544	an effective way to enhance stem-cell therapy	1529:1573	Silicate based bioactive hydrogels activated MSCs and cell-cell interactions in cardiac tissue after AMI and significantly enhanced the efficacy, which suggests that this bioactive hydrogel based approach is an effective way to enhance stem-cell therapy.
32724972	1	37	theme	infarction	181:190	arg1	treatment					197:205	myocardial infarction (MI) treatment	170:205	myocardial infarction (MI) treatment	170:205	Stem-cell therapy has been proved as a promising strategy for myocardial infarction (MI) treatment.
32724972	4	38	theme	CM	804:805	arg1	experiment					818:827	a MSC and CM co-culture experiment	794:827	experiment	818:827	And the effect of the composite hydrogel on MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo and the possible mechanism of the BG/γ-PGA/CS hydrogel for the stimulation of the intercellular interaction between MSCs and cardiomyocytes (CMs) was explored by a MSC and CM co-culture experiment in vitro.
32724972	2	39	theme	cellular	267:274	arg1	activity					276:283	the cellular activity	263:283	the cellular activity of transplanted mesenchymal stem cells (MSCs)	263:329	However, the therapeutic efficacy is mainly limited by the cellular activity of transplanted mesenchymal stem cells (MSCs).
32724972	7	40	theme	bioactive	1336:1344	arg1	hydrogels					1346:1354	Silicate based bioactive hydrogels	1321:1354	Silicate based bioactive hydrogels	1321:1354	Silicate based bioactive hydrogels activated MSCs and cell-cell interactions in cardiac tissue after AMI and significantly enhanced the efficacy, which suggests that this bioactive hydrogel based approach is an effective way to enhance stem-cell therapy.
32724972	4	41	theme	acute	584:588	arg1	AMI					613:615	AMI	613:615	AMI	613:615	And the effect of the composite hydrogel on MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo and the possible mechanism of the BG/γ-PGA/CS hydrogel for the stimulation of the intercellular interaction between MSCs and cardiomyocytes (CMs) was explored by a MSC and CM co-culture experiment in vitro.
32724972	4	41	theme	acute	584:588	arg1	infarction					601:610	a rat acute myocardial infarction	578:610	a rat acute myocardial infarction (AMI) model	578:622	And the effect of the composite hydrogel on MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo and the possible mechanism of the BG/γ-PGA/CS hydrogel for the stimulation of the intercellular interaction between MSCs and cardiomyocytes (CMs) was explored by a MSC and CM co-culture experiment in vitro.
32724972	2	42	theme	cells	318:322	arg1	activity					276:283	the cellular activity	263:283	the cellular activity of transplanted mesenchymal stem cells (MSCs)	263:329	However, the therapeutic efficacy is mainly limited by the cellular activity of transplanted mesenchymal stem cells (MSCs).
32724972	4	43	theme	co-culture	807:816	arg1	experiment					818:827	a MSC and CM co-culture experiment	794:827	experiment	818:827	And the effect of the composite hydrogel on MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo and the possible mechanism of the BG/γ-PGA/CS hydrogel for the stimulation of the intercellular interaction between MSCs and cardiomyocytes (CMs) was explored by a MSC and CM co-culture experiment in vitro.
32724972	5	44	theme	hydrogel	896:903	arg1	implantation					843:854	The implantation	839:854	The implantation of the MSC loaded BG/γ-PGA/CS composite hydrogel in the mice AMI model	839:925	The implantation of the MSC loaded BG/γ-PGA/CS composite hydrogel in the mice AMI model showed a significant improvement in the therapeutic efficacy with improved cardiac function, attenuation of heart remodeling, reduced cardiomyocyte apoptosis and accelerated vascularization.
32724972	3	45	theme	/γ-polyglutamic	368:382	arg1	hydrogel					411:418	a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel	347:418	a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel	347:418	In this study, a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel was obtained by in situ adding BG to stimulate the imine bond formation.
32724972	3	46	theme	γ-PGA	390:394	arg1	hydrogel					411:418	a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel	347:418	a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel	347:418	In this study, a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel was obtained by in situ adding BG to stimulate the imine bond formation.
32724972	3	47	theme	acid	384:387	arg1	hydrogel					411:418	a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel	347:418	a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel	347:418	In this study, a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel was obtained by in situ adding BG to stimulate the imine bond formation.
32724972	7	48	theme	stem-cell	1557:1565	arg1	therapy					1567:1573	stem-cell therapy	1557:1573	stem-cell therapy	1557:1573	Silicate based bioactive hydrogels activated MSCs and cell-cell interactions in cardiac tissue after AMI and significantly enhanced the efficacy, which suggests that this bioactive hydrogel based approach is an effective way to enhance stem-cell therapy.
32724972	0	49	theme	stem	30:33	arg1	cells					35:39	mesenchymal stem cells	18:39	mesenchymal stem cells (MSCs)	18:46	In situ activated mesenchymal stem cells (MSCs) by bioactive hydrogels for myocardial infarction treatment.
32724972	0	49	theme	stem	30:33	arg1	MSCs					42:45	MSCs	42:45	MSCs	42:45	In situ activated mesenchymal stem cells (MSCs) by bioactive hydrogels for myocardial infarction treatment.
32724972	6	50	theme	cell	1131:1134	arg1	experiments					1136:1146	The in vitro cell experiments	1118:1146	The in vitro cell experiments	1118:1146	The in vitro cell experiments demonstrated that the BG/γ-PGA/CS hydrogel activated the intercellular interaction between MSCs and CMs, which resulted in reduced cell apoptosis and enhanced angiogenesis.
32724972	5	51	from	improvement	948:958	arg1	efficacy					979:986	the therapeutic efficacy	963:986	the therapeutic efficacy with improved cardiac function, attenuation of heart remodeling, reduced cardiomyocyte apoptosis and accelerated vascularization	963:1115	The implantation of the MSC loaded BG/γ-PGA/CS composite hydrogel in the mice AMI model showed a significant improvement in the therapeutic efficacy with improved cardiac function, attenuation of heart remodeling, reduced cardiomyocyte apoptosis and accelerated vascularization.
32724972	4	52	theme	therapeutic	540:550	arg1	efficacy					552:559	MI therapeutic efficacy	537:559	MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo	537:630	And the effect of the composite hydrogel on MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo and the possible mechanism of the BG/γ-PGA/CS hydrogel for the stimulation of the intercellular interaction between MSCs and cardiomyocytes (CMs) was explored by a MSC and CM co-culture experiment in vitro.
32724972	3	53	theme	novel	349:353	arg1	hydrogel					411:418	a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel	347:418	a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel	347:418	In this study, a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel was obtained by in situ adding BG to stimulate the imine bond formation.
32724972	4	54	from	effect	501:506	arg1	efficacy					552:559	MI therapeutic efficacy	537:559	MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo	537:630	And the effect of the composite hydrogel on MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo and the possible mechanism of the BG/γ-PGA/CS hydrogel for the stimulation of the intercellular interaction between MSCs and cardiomyocytes (CMs) was explored by a MSC and CM co-culture experiment in vitro.
32724972	4	55	from	MSC	796:798	arg1	vitro					832:836	vitro	832:836	vitro	832:836	And the effect of the composite hydrogel on MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo and the possible mechanism of the BG/γ-PGA/CS hydrogel for the stimulation of the intercellular interaction between MSCs and cardiomyocytes (CMs) was explored by a MSC and CM co-culture experiment in vitro.
32724972	4	56	theme	intercellular	714:726	arg1	interaction					728:738	the intercellular interaction	710:738	the intercellular interaction between MSCs and cardiomyocytes (CMs)	710:776	And the effect of the composite hydrogel on MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo and the possible mechanism of the BG/γ-PGA/CS hydrogel for the stimulation of the intercellular interaction between MSCs and cardiomyocytes (CMs) was explored by a MSC and CM co-culture experiment in vitro.
32724972	3	57	theme	bioglass	355:362	arg1	hydrogel					411:418	a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel	347:418	a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel	347:418	In this study, a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel was obtained by in situ adding BG to stimulate the imine bond formation.
32724972	5	58	theme	remodeling	1041:1050	arg1	function					1010:1017	improved cardiac function	993:1017	improved cardiac function	993:1017	The implantation of the MSC loaded BG/γ-PGA/CS composite hydrogel in the mice AMI model showed a significant improvement in the therapeutic efficacy with improved cardiac function, attenuation of heart remodeling, reduced cardiomyocyte apoptosis and accelerated vascularization.
32724972	5	58	theme	remodeling	1041:1050	arg1	apoptosis					1075:1083	reduced cardiomyocyte apoptosis	1053:1083	reduced cardiomyocyte apoptosis	1053:1083	The implantation of the MSC loaded BG/γ-PGA/CS composite hydrogel in the mice AMI model showed a significant improvement in the therapeutic efficacy with improved cardiac function, attenuation of heart remodeling, reduced cardiomyocyte apoptosis and accelerated vascularization.
32724972	5	58	theme	remodeling	1041:1050	arg1	vascularization					1101:1115	accelerated vascularization	1089:1115	accelerated vascularization	1089:1115	The implantation of the MSC loaded BG/γ-PGA/CS composite hydrogel in the mice AMI model showed a significant improvement in the therapeutic efficacy with improved cardiac function, attenuation of heart remodeling, reduced cardiomyocyte apoptosis and accelerated vascularization.
32724972	5	58	theme	remodeling	1041:1050	arg1	attenuation					1020:1030	attenuation	1020:1030	attenuation of heart remodeling	1020:1050	The implantation of the MSC loaded BG/γ-PGA/CS composite hydrogel in the mice AMI model showed a significant improvement in the therapeutic efficacy with improved cardiac function, attenuation of heart remodeling, reduced cardiomyocyte apoptosis and accelerated vascularization.
32724972	5	59	theme	cardiac	1002:1008	arg1	function					1010:1017	improved cardiac function	993:1017	improved cardiac function	993:1017	The implantation of the MSC loaded BG/γ-PGA/CS composite hydrogel in the mice AMI model showed a significant improvement in the therapeutic efficacy with improved cardiac function, attenuation of heart remodeling, reduced cardiomyocyte apoptosis and accelerated vascularization.
32724972	5	60	theme	AMI	917:919	arg1	model					921:925	the mice AMI model	908:925	the mice AMI model	908:925	The implantation of the MSC loaded BG/γ-PGA/CS composite hydrogel in the mice AMI model showed a significant improvement in the therapeutic efficacy with improved cardiac function, attenuation of heart remodeling, reduced cardiomyocyte apoptosis and accelerated vascularization.
32724972	5	61	theme	mice	912:915	arg1	model					921:925	the mice AMI model	908:925	the mice AMI model	908:925	The implantation of the MSC loaded BG/γ-PGA/CS composite hydrogel in the mice AMI model showed a significant improvement in the therapeutic efficacy with improved cardiac function, attenuation of heart remodeling, reduced cardiomyocyte apoptosis and accelerated vascularization.
32724972	1	62	theme	Stem-cell	108:116	arg1	therapy					118:124	Stem-cell therapy	108:124	Stem-cell therapy	108:124	Stem-cell therapy has been proved as a promising strategy for myocardial infarction (MI) treatment.
32724972	5	63	theme	composite	886:894	arg1	hydrogel					896:903	the MSC loaded BG/γ-PGA/CS composite hydrogel	859:903	the MSC loaded BG/γ-PGA/CS composite hydrogel	859:903	The implantation of the MSC loaded BG/γ-PGA/CS composite hydrogel in the mice AMI model showed a significant improvement in the therapeutic efficacy with improved cardiac function, attenuation of heart remodeling, reduced cardiomyocyte apoptosis and accelerated vascularization.
32724972	4	64	theme	hydrogel	678:685	arg1	mechanism					649:657	the possible mechanism	636:657	the possible mechanism of the BG/γ-PGA/CS hydrogel for the stimulation of the intercellular interaction between MSCs and cardiomyocytes (CMs)	636:776	And the effect of the composite hydrogel on MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo and the possible mechanism of the BG/γ-PGA/CS hydrogel for the stimulation of the intercellular interaction between MSCs and cardiomyocytes (CMs) was explored by a MSC and CM co-culture experiment in vitro.
32724972	4	64	theme	hydrogel	678:685	arg1	effect					501:506	the effect	497:506	the effect of the composite hydrogel on MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo	497:630	And the effect of the composite hydrogel on MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo and the possible mechanism of the BG/γ-PGA/CS hydrogel for the stimulation of the intercellular interaction between MSCs and cardiomyocytes (CMs) was explored by a MSC and CM co-culture experiment in vitro.
32724972	2	65	theme	stem	313:316	arg1	cells					318:322	transplanted mesenchymal stem cells	288:322	transplanted mesenchymal stem cells (MSCs)	288:329	However, the therapeutic efficacy is mainly limited by the cellular activity of transplanted mesenchymal stem cells (MSCs).
32724972	2	65	theme	stem	313:316	arg1	MSCs					325:328	MSCs	325:328	MSCs	325:328	However, the therapeutic efficacy is mainly limited by the cellular activity of transplanted mesenchymal stem cells (MSCs).
32724972	7	66	theme	Silicate	1321:1328	arg1	hydrogels					1346:1354	Silicate based bioactive hydrogels	1321:1354	Silicate based bioactive hydrogels	1321:1354	Silicate based bioactive hydrogels activated MSCs and cell-cell interactions in cardiac tissue after AMI and significantly enhanced the efficacy, which suggests that this bioactive hydrogel based approach is an effective way to enhance stem-cell therapy.
32724972	5	67	dep	loaded	867:872	arg1	MSC					863:865	MSC	863:865	MSC	863:865	The implantation of the MSC loaded BG/γ-PGA/CS composite hydrogel in the mice AMI model showed a significant improvement in the therapeutic efficacy with improved cardiac function, attenuation of heart remodeling, reduced cardiomyocyte apoptosis and accelerated vascularization.
32724972	4	68	theme	BG/γ-PGA/CS	666:676	arg1	hydrogel					678:685	the BG/γ-PGA/CS hydrogel	662:685	the BG/γ-PGA/CS hydrogel	662:685	And the effect of the composite hydrogel on MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo and the possible mechanism of the BG/γ-PGA/CS hydrogel for the stimulation of the intercellular interaction between MSCs and cardiomyocytes (CMs) was explored by a MSC and CM co-culture experiment in vitro.
32724972	2	69	theme	mesenchymal	301:311	arg1	cells					318:322	transplanted mesenchymal stem cells	288:322	transplanted mesenchymal stem cells (MSCs)	288:329	However, the therapeutic efficacy is mainly limited by the cellular activity of transplanted mesenchymal stem cells (MSCs).
32724972	2	69	theme	mesenchymal	301:311	arg1	MSCs					325:328	MSCs	325:328	MSCs	325:328	However, the therapeutic efficacy is mainly limited by the cellular activity of transplanted mesenchymal stem cells (MSCs).
32724972	6	70	theme	cell	1279:1282	arg1	apoptosis					1284:1292	reduced cell apoptosis	1271:1292	reduced cell apoptosis	1271:1292	The in vitro cell experiments demonstrated that the BG/γ-PGA/CS hydrogel activated the intercellular interaction between MSCs and CMs, which resulted in reduced cell apoptosis and enhanced angiogenesis.
32724972	3	71	theme	CS	407:408	arg1	hydrogel					411:418	a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel	347:418	a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel	347:418	In this study, a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel was obtained by in situ adding BG to stimulate the imine bond formation.
32724972	5	72	theme	heart	1035:1039	arg1	remodeling					1041:1050	heart remodeling	1035:1050	heart remodeling	1035:1050	The implantation of the MSC loaded BG/γ-PGA/CS composite hydrogel in the mice AMI model showed a significant improvement in the therapeutic efficacy with improved cardiac function, attenuation of heart remodeling, reduced cardiomyocyte apoptosis and accelerated vascularization.
32724972	5	73	from	implantation	843:854	arg1	model					921:925	the mice AMI model	908:925	the mice AMI model	908:925	The implantation of the MSC loaded BG/γ-PGA/CS composite hydrogel in the mice AMI model showed a significant improvement in the therapeutic efficacy with improved cardiac function, attenuation of heart remodeling, reduced cardiomyocyte apoptosis and accelerated vascularization.
32724972	2	74	theme	therapeutic	221:231	arg1	efficacy					233:240	the therapeutic efficacy	217:240	the therapeutic efficacy	217:240	However, the therapeutic efficacy is mainly limited by the cellular activity of transplanted mesenchymal stem cells (MSCs).
32724972	3	75	theme	/chitosan	396:404	arg1	hydrogel					411:418	a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel	347:418	a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel	347:418	In this study, a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel was obtained by in situ adding BG to stimulate the imine bond formation.
32724972	3	76	theme	in	436:437	arg1	BG					451:452	in situ adding BG to stimulate the imine bond formation	436:490	in situ adding BG to stimulate the imine bond formation	436:490	In this study, a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel was obtained by in situ adding BG to stimulate the imine bond formation.
32724972	5	77	with	efficacy	979:986	arg1	function					1010:1017	improved cardiac function	993:1017	improved cardiac function	993:1017	The implantation of the MSC loaded BG/γ-PGA/CS composite hydrogel in the mice AMI model showed a significant improvement in the therapeutic efficacy with improved cardiac function, attenuation of heart remodeling, reduced cardiomyocyte apoptosis and accelerated vascularization.
32724972	5	77	with	efficacy	979:986	arg1	apoptosis					1075:1083	reduced cardiomyocyte apoptosis	1053:1083	reduced cardiomyocyte apoptosis	1053:1083	The implantation of the MSC loaded BG/γ-PGA/CS composite hydrogel in the mice AMI model showed a significant improvement in the therapeutic efficacy with improved cardiac function, attenuation of heart remodeling, reduced cardiomyocyte apoptosis and accelerated vascularization.
32724972	5	77	with	efficacy	979:986	arg1	vascularization					1101:1115	accelerated vascularization	1089:1115	accelerated vascularization	1089:1115	The implantation of the MSC loaded BG/γ-PGA/CS composite hydrogel in the mice AMI model showed a significant improvement in the therapeutic efficacy with improved cardiac function, attenuation of heart remodeling, reduced cardiomyocyte apoptosis and accelerated vascularization.
32724972	5	77	with	efficacy	979:986	arg1	attenuation					1020:1030	attenuation	1020:1030	attenuation of heart remodeling	1020:1050	The implantation of the MSC loaded BG/γ-PGA/CS composite hydrogel in the mice AMI model showed a significant improvement in the therapeutic efficacy with improved cardiac function, attenuation of heart remodeling, reduced cardiomyocyte apoptosis and accelerated vascularization.
32724972	4	78	theme	hydrogel	525:532	arg1	mechanism					649:657	the possible mechanism	636:657	the possible mechanism of the BG/γ-PGA/CS hydrogel for the stimulation of the intercellular interaction between MSCs and cardiomyocytes (CMs)	636:776	And the effect of the composite hydrogel on MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo and the possible mechanism of the BG/γ-PGA/CS hydrogel for the stimulation of the intercellular interaction between MSCs and cardiomyocytes (CMs) was explored by a MSC and CM co-culture experiment in vitro.
32724972	4	78	theme	hydrogel	525:532	arg1	effect					501:506	the effect	497:506	the effect of the composite hydrogel on MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo	497:630	And the effect of the composite hydrogel on MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo and the possible mechanism of the BG/γ-PGA/CS hydrogel for the stimulation of the intercellular interaction between MSCs and cardiomyocytes (CMs) was explored by a MSC and CM co-culture experiment in vitro.
32724972	3	79	theme	BG	365:366	arg1	hydrogel					411:418	a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel	347:418	a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel	347:418	In this study, a novel bioglass (BG)/γ-polyglutamic acid (γ-PGA)/chitosan (CS) hydrogel was obtained by in situ adding BG to stimulate the imine bond formation.
32724972	4	80	theme	MI	537:538	arg1	efficacy					552:559	MI therapeutic efficacy	537:559	MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo	537:630	And the effect of the composite hydrogel on MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo and the possible mechanism of the BG/γ-PGA/CS hydrogel for the stimulation of the intercellular interaction between MSCs and cardiomyocytes (CMs) was explored by a MSC and CM co-culture experiment in vitro.
32724972	4	81	theme	rat	580:582	arg1	AMI					613:615	AMI	613:615	AMI	613:615	And the effect of the composite hydrogel on MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo and the possible mechanism of the BG/γ-PGA/CS hydrogel for the stimulation of the intercellular interaction between MSCs and cardiomyocytes (CMs) was explored by a MSC and CM co-culture experiment in vitro.
32724972	4	81	theme	rat	580:582	arg1	infarction					601:610	a rat acute myocardial infarction	578:610	a rat acute myocardial infarction (AMI) model	578:622	And the effect of the composite hydrogel on MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo and the possible mechanism of the BG/γ-PGA/CS hydrogel for the stimulation of the intercellular interaction between MSCs and cardiomyocytes (CMs) was explored by a MSC and CM co-culture experiment in vitro.
32724972	7	82	theme	based	1330:1334	arg1	hydrogels					1346:1354	Silicate based bioactive hydrogels	1321:1354	Silicate based bioactive hydrogels	1321:1354	Silicate based bioactive hydrogels activated MSCs and cell-cell interactions in cardiac tissue after AMI and significantly enhanced the efficacy, which suggests that this bioactive hydrogel based approach is an effective way to enhance stem-cell therapy.
32724972	4	83	theme	composite	515:523	arg1	hydrogel					525:532	the composite hydrogel	511:532	the composite hydrogel	511:532	And the effect of the composite hydrogel on MI therapeutic efficacy was evaluated in a rat acute myocardial infarction (AMI) model in vivo and the possible mechanism of the BG/γ-PGA/CS hydrogel for the stimulation of the intercellular interaction between MSCs and cardiomyocytes (CMs) was explored by a MSC and CM co-culture experiment in vitro.
33288623	3	0	from	crescentus	534:543	arg1	present					520:526	present	520:526	present	520:526	It is commonly thought that the six paralogs of the flagellin gene present in C. crescentus are important for bacteriophage evasion.
33288623	8	1	from	modifications	1388:1400	arg1	residues					1433:1440	conserved surface threonine residues	1405:1440	conserved surface threonine residues of FljK	1405:1448	We observe posttranslational modifications on conserved surface threonine residues of FljK that are likely O-linked glycans.
33288623	8	1	from	modifications	1388:1400	arg1	FljK					1445:1448	FljK	1445:1448	FljK	1445:1448	We observe posttranslational modifications on conserved surface threonine residues of FljK that are likely O-linked glycans.
33288623	6	2	theme	most	944:947	arg1	strains					958:964	most deletion strains	944:964	most deletion strains	944:964	Interestingly, we observe that most deletion strains still generate flagellar filaments, with the exception of a strain that contains only the most divergent flagellin, FljJ, or a strain that contains only FljN and FljO.
33288623	4	3	theme	flagellins	626:635	arg1	deletion					605:612	deletion	605:612	deletion of specific flagellins in C. crescentus	605:652	Here, we show that deletion of specific flagellins in C. crescentus can indeed attenuate ϕCbK adsorption efficiency, although no single deletion completely ablates ϕCbK adsorption.
33288623	5	4	theme	common	816:821	arg1	motif					823:827	a common motif	814:827	a common motif among the six known flagellins in C. crescentus	814:875	Thus, the bacteriophage ϕCbK likely recognizes a common motif among the six known flagellins in C. crescentus with various degrees of efficiency.
33288623	5	5	theme	efficiency	901:910	arg1	degrees					890:896	various degrees	882:896	various degrees of efficiency	882:910	Thus, the bacteriophage ϕCbK likely recognizes a common motif among the six known flagellins in C. crescentus with various degrees of efficiency.
33288623	12	6	theme	flagellin	2016:2024	arg1	genes					2026:2030	numerous flagellin genes	2007:2030	numerous flagellin genes	2007:2030	Some bacteria harbor numerous flagellin genes and are therefore able to generate flagellar filaments with complex compositions, which is thought to be important for evasion from bacteriophages.
33288623	1	7	theme	oligotrophic	218:229	arg1	environments					252:263	oligotrophic fresh- and saltwater environments	218:263	oligotrophic fresh- and saltwater environments	218:263	Caulobacter crescentus is a Gram-negative alphaproteobacterium that commonly lives in oligotrophic fresh- and saltwater environments.
33288623	8	8	theme	O-linked	1466:1473	arg1	modifications					1388:1400	posttranslational modifications	1370:1400	posttranslational modifications on conserved surface threonine residues of FljK that are likely O-linked glycans	1370:1481	We observe posttranslational modifications on conserved surface threonine residues of FljK that are likely O-linked glycans.
33288623	8	8	theme	O-linked	1466:1473	arg1	glycans					1475:1481	likely O-linked glycans	1459:1481	likely O-linked glycans	1459:1481	We observe posttranslational modifications on conserved surface threonine residues of FljK that are likely O-linked glycans.
33288623	2	9	theme	pilus	418:422	arg1	complexes					424:432	pilus complexes	418:432	pilus complexes	418:432	C. crescentus is a host to many bacteriophages, including ϕCbK and ϕCbK-like bacteriophages, which require interaction with the bacterial flagellum and pilus complexes during adsorption.
33288623	4	10	theme	adsorption	680:689	arg1	efficiency					691:700	ϕCbK adsorption efficiency	675:700	ϕCbK adsorption efficiency	675:700	Here, we show that deletion of specific flagellins in C. crescentus can indeed attenuate ϕCbK adsorption efficiency, although no single deletion completely ablates ϕCbK adsorption.
33288623	10	11	theme	FljK	1661:1664	arg1	mixture					1650:1656	a heterogeneous mixture	1634:1656	a heterogeneous mixture	1634:1656	We also determined the structure of a filament composed of a heterogeneous mixture of FljK and FljL, the final resolution of which was limited to approximately 4.6 Å.
33288623	15	12	theme	future	2540:2545	arg1	studies					2547:2553	future studies	2540:2553	future studies on determining how bacteriophage ϕCbK attaches to flagellar filaments at the molecular level	2540:2646	We also present a high-resolution structure of a flagellar filament containing only the FljK flagellin, which provides a platform for future studies on determining how bacteriophage ϕCbK attaches to flagellar filaments at the molecular level.
33288623	10	13	theme	mixture	1650:1656	arg1	limited					1710:1716	limited	1710:1716	limited	1710:1716	We also determined the structure of a filament composed of a heterogeneous mixture of FljK and FljL, the final resolution of which was limited to approximately 4.6 Å.
33288623	10	13	theme	mixture	1650:1656	arg1	resolution					1686:1695	the final resolution	1676:1695	the final resolution	1676:1695	We also determined the structure of a filament composed of a heterogeneous mixture of FljK and FljL, the final resolution of which was limited to approximately 4.6 Å.
33288623	14	14	theme	bacteriophage	2386:2398	arg1	ϕCbK					2400:2403	bacteriophage ϕCbK	2386:2403	bacteriophage ϕCbK	2386:2403	We find that filaments containing the FljK flagellin are the preferred substrate for bacteriophage ϕCbK.
33288623	9	15	theme	ϕCbK	1545:1548	arg1	adsorption					1550:1559	ϕCbK adsorption	1545:1559	ϕCbK adsorption	1545:1559	The possibility of interplay between these modifications and ϕCbK adsorption is discussed.
33288623	7	16	theme	filament	1349:1356	arg1	straightening					1328:1340	straightening	1328:1340	straightening of the filament	1328:1356	To visualize the surface residues that are likely recognized by ϕCbK, we determined two high-resolution structures of the FljK filament, with and without an amino acid substitution that induces straightening of the filament.
33288623	15	17	theme	FljK	2494:2497	arg1	flagellin					2499:2507	only the FljK flagellin	2485:2507	only the FljK flagellin	2485:2507	We also present a high-resolution structure of a flagellar filament containing only the FljK flagellin, which provides a platform for future studies on determining how bacteriophage ϕCbK attaches to flagellar filaments at the molecular level.
33288623	1	18	theme	Gram-negative	160:172	arg1	crescentus					144:153	Caulobacter crescentus	132:153	Caulobacter crescentus	132:153	Caulobacter crescentus is a Gram-negative alphaproteobacterium that commonly lives in oligotrophic fresh- and saltwater environments.
33288623	1	18	theme	Gram-negative	160:172	arg1	alphaproteobacterium					174:193	a Gram-negative alphaproteobacterium	158:193	a Gram-negative alphaproteobacterium that commonly lives in oligotrophic fresh- and saltwater environments	158:263	Caulobacter crescentus is a Gram-negative alphaproteobacterium that commonly lives in oligotrophic fresh- and saltwater environments.
33288623	0	19	theme	Phage	84:88	arg1	Predation					94:102	Phage CbK Predation	84:102	Phage CbK Predation of Multiflagellin Bacteria	84:129	Flagellar Structures from the Bacterium Caulobacter crescentus and Implications for Phage CbK Predation of Multiflagellin Bacteria.
33288623	5	20	from	motif	823:827	arg1	crescentus					866:875	C. crescentus	863:875	C. crescentus	863:875	Thus, the bacteriophage ϕCbK likely recognizes a common motif among the six known flagellins in C. crescentus with various degrees of efficiency.
33288623	15	21	theme	filament	2465:2472	arg1	structure					2440:2448	a high-resolution structure	2422:2448	a high-resolution structure of a flagellar filament containing only the FljK flagellin, which provides a platform for future studies on determining how bacteriophage ϕCbK attaches to flagellar filaments at the molecular level	2422:2646	We also present a high-resolution structure of a flagellar filament containing only the FljK flagellin, which provides a platform for future studies on determining how bacteriophage ϕCbK attaches to flagellar filaments at the molecular level.
33288623	11	22	dep	level.IMPORTANCE	1865:1880	arg1	serve					1912:1916	serve	1912:1916	serve as an initial attachment point for many bacteriophages to bacteria	1912:1983	Altogether, this work builds a platform for future investigations of how phage ϕCbK infects C. crescentus at the molecular level.IMPORTANCE Bacterial flagellar filaments serve as an initial attachment point for many bacteriophages to bacteria.
33288623	0	23	from	crescentus	52:61	arg1	Implications					67:78	Implications	67:78	Implications for Phage CbK Predation of Multiflagellin Bacteria	67:129	Flagellar Structures from the Bacterium Caulobacter crescentus and Implications for Phage CbK Predation of Multiflagellin Bacteria.
33288623	0	23	from	crescentus	52:61	arg1	Structures					10:19	Flagellar Structures	0:19	Flagellar Structures from the Bacterium Caulobacter crescentus	0:61	Flagellar Structures from the Bacterium Caulobacter crescentus and Implications for Phage CbK Predation of Multiflagellin Bacteria.
33288623	10	24	theme	final	1680:1684	arg1	limited					1710:1716	limited	1710:1716	limited	1710:1716	We also determined the structure of a filament composed of a heterogeneous mixture of FljK and FljL, the final resolution of which was limited to approximately 4.6 Å.
33288623	10	24	theme	final	1680:1684	arg1	resolution					1686:1695	the final resolution	1676:1695	the final resolution	1676:1695	We also determined the structure of a filament composed of a heterogeneous mixture of FljK and FljL, the final resolution of which was limited to approximately 4.6 Å.
33288623	4	25	from	deletion	605:612	arg1	crescentus					643:652	C. crescentus	640:652	C. crescentus	640:652	Here, we show that deletion of specific flagellins in C. crescentus can indeed attenuate ϕCbK adsorption efficiency, although no single deletion completely ablates ϕCbK adsorption.
33288623	6	26	theme	strain	1093:1098	arg1	exception					1011:1019	the exception	1007:1019	the exception of a strain that contains only the most divergent flagellin, FljJ, or a strain that contains only FljN and FljO	1007:1131	Interestingly, we observe that most deletion strains still generate flagellar filaments, with the exception of a strain that contains only the most divergent flagellin, FljJ, or a strain that contains only FljN and FljO.
33288623	15	27	from	level	2642:2646	arg1	filaments					2615:2623	flagellar filaments	2605:2623	flagellar filaments at the molecular level	2605:2646	We also present a high-resolution structure of a flagellar filament containing only the FljK flagellin, which provides a platform for future studies on determining how bacteriophage ϕCbK attaches to flagellar filaments at the molecular level.
33288623	11	28	theme	initial	1924:1930	arg1	point					1943:1947	an initial attachment point	1921:1947	an initial attachment point for many bacteriophages to bacteria	1921:1983	Altogether, this work builds a platform for future investigations of how phage ϕCbK infects C. crescentus at the molecular level.IMPORTANCE Bacterial flagellar filaments serve as an initial attachment point for many bacteriophages to bacteria.
33288623	11	28	theme	initial	1924:1930	arg1	filaments					1902:1910	Bacterial flagellar filaments	1882:1910	Bacterial flagellar filaments	1882:1910	Altogether, this work builds a platform for future investigations of how phage ϕCbK infects C. crescentus at the molecular level.IMPORTANCE Bacterial flagellar filaments serve as an initial attachment point for many bacteriophages to bacteria.
33288623	0	29	theme	Flagellar	0:8	arg1	Structures					10:19	Flagellar Structures	0:19	Flagellar Structures from the Bacterium Caulobacter crescentus	0:61	Flagellar Structures from the Bacterium Caulobacter crescentus and Implications for Phage CbK Predation of Multiflagellin Bacteria.
33288623	8	30	link	O-linked	1466:1473	arg1	modifications					1388:1400	posttranslational modifications	1370:1400	posttranslational modifications on conserved surface threonine residues of FljK that are likely O-linked glycans	1370:1481	We observe posttranslational modifications on conserved surface threonine residues of FljK that are likely O-linked glycans.
33288623	8	30	link	O-linked	1466:1473	arg1	glycans					1475:1481	likely O-linked glycans	1459:1481	likely O-linked glycans	1459:1481	We observe posttranslational modifications on conserved surface threonine residues of FljK that are likely O-linked glycans.
33288623	13	31	from	importance	2209:2218	arg1	crescentus					2253:2262	C. crescentus	2250:2262	C. crescentus	2250:2262	This study characterizes the importance of the six flagellin genes in C. crescentus for infection by bacteriophage ϕCbK.
33288623	8	32	theme	conserved	1405:1413	arg1	residues					1433:1440	conserved surface threonine residues	1405:1440	conserved surface threonine residues of FljK	1405:1448	We observe posttranslational modifications on conserved surface threonine residues of FljK that are likely O-linked glycans.
33288623	8	32	theme	conserved	1405:1413	arg1	FljK					1445:1448	FljK	1445:1448	FljK	1445:1448	We observe posttranslational modifications on conserved surface threonine residues of FljK that are likely O-linked glycans.
33288623	13	33	theme	bacteriophage	2281:2293	arg1	ϕCbK					2295:2298	bacteriophage ϕCbK	2281:2298	bacteriophage ϕCbK	2281:2298	This study characterizes the importance of the six flagellin genes in C. crescentus for infection by bacteriophage ϕCbK.
33288623	8	34	theme	threonine	1423:1431	arg1	residues					1433:1440	conserved surface threonine residues	1405:1440	conserved surface threonine residues of FljK	1405:1448	We observe posttranslational modifications on conserved surface threonine residues of FljK that are likely O-linked glycans.
33288623	8	34	theme	threonine	1423:1431	arg1	FljK					1445:1448	FljK	1445:1448	FljK	1445:1448	We observe posttranslational modifications on conserved surface threonine residues of FljK that are likely O-linked glycans.
33288623	11	35	theme	many	1953:1956	arg1	bacteriophages					1958:1971	many bacteriophages	1953:1971	many bacteriophages to bacteria	1953:1983	Altogether, this work builds a platform for future investigations of how phage ϕCbK infects C. crescentus at the molecular level.IMPORTANCE Bacterial flagellar filaments serve as an initial attachment point for many bacteriophages to bacteria.
33288623	0	36	theme	Bacterium	30:38	arg1	crescentus					52:61	the Bacterium Caulobacter crescentus	26:61	the Bacterium Caulobacter crescentus	26:61	Flagellar Structures from the Bacterium Caulobacter crescentus and Implications for Phage CbK Predation of Multiflagellin Bacteria.
33288623	7	37	theme	acid	1297:1300	arg1	substitution					1302:1313	an amino acid substitution	1288:1313	an amino acid substitution that induces straightening of the filament	1288:1356	To visualize the surface residues that are likely recognized by ϕCbK, we determined two high-resolution structures of the FljK filament, with and without an amino acid substitution that induces straightening of the filament.
33288623	15	38	attach	attaches	2593:2600	arg1	filaments					2615:2623	flagellar filaments	2605:2623	flagellar filaments at the molecular level	2605:2646	We also present a high-resolution structure of a flagellar filament containing only the FljK flagellin, which provides a platform for future studies on determining how bacteriophage ϕCbK attaches to flagellar filaments at the molecular level.
33288623	15	38	attach	attaches	2593:2600	arg2	ϕCbK					2588:2591	bacteriophage ϕCbK	2574:2591	bacteriophage ϕCbK	2574:2591	We also present a high-resolution structure of a flagellar filament containing only the FljK flagellin, which provides a platform for future studies on determining how bacteriophage ϕCbK attaches to flagellar filaments at the molecular level.
33288623	11	39	theme	Bacterial	1882:1890	arg1	point					1943:1947	an initial attachment point	1921:1947	an initial attachment point for many bacteriophages to bacteria	1921:1983	Altogether, this work builds a platform for future investigations of how phage ϕCbK infects C. crescentus at the molecular level.IMPORTANCE Bacterial flagellar filaments serve as an initial attachment point for many bacteriophages to bacteria.
33288623	11	39	theme	Bacterial	1882:1890	arg1	filaments					1902:1910	Bacterial flagellar filaments	1882:1910	Bacterial flagellar filaments	1882:1910	Altogether, this work builds a platform for future investigations of how phage ϕCbK infects C. crescentus at the molecular level.IMPORTANCE Bacterial flagellar filaments serve as an initial attachment point for many bacteriophages to bacteria.
33288623	7	40	theme	surface	1151:1157	arg1	residues					1159:1166	the surface residues	1147:1166	the surface residues that are likely recognized by ϕCbK	1147:1201	To visualize the surface residues that are likely recognized by ϕCbK, we determined two high-resolution structures of the FljK filament, with and without an amino acid substitution that induces straightening of the filament.
33288623	3	41	theme	gene	515:518	arg1	important					549:557	important	549:557	important	549:557	It is commonly thought that the six paralogs of the flagellin gene present in C. crescentus are important for bacteriophage evasion.
33288623	3	41	theme	gene	515:518	arg1	paralogs					489:496	the six paralogs	481:496	the six paralogs of the flagellin gene present in C. crescentus	481:543	It is commonly thought that the six paralogs of the flagellin gene present in C. crescentus are important for bacteriophage evasion.
33288623	4	42	theme	single	715:720	arg1	deletion					722:729	no single deletion	712:729	no single deletion	712:729	Here, we show that deletion of specific flagellins in C. crescentus can indeed attenuate ϕCbK adsorption efficiency, although no single deletion completely ablates ϕCbK adsorption.
33288623	13	43	theme	flagellin	2231:2239	arg1	genes					2241:2245	the six flagellin genes	2223:2245	the six flagellin genes	2223:2245	This study characterizes the importance of the six flagellin genes in C. crescentus for infection by bacteriophage ϕCbK.
33288623	2	44	with	complexes	424:432	arg1	flagellum					404:412	the bacterial flagellum	390:412	the bacterial flagellum	390:412	C. crescentus is a host to many bacteriophages, including ϕCbK and ϕCbK-like bacteriophages, which require interaction with the bacterial flagellum and pilus complexes during adsorption.
33288623	3	45	attach	present	520:526	arg2	gene					515:518	the flagellin gene	501:518	the flagellin gene present in C. crescentus	501:543	It is commonly thought that the six paralogs of the flagellin gene present in C. crescentus are important for bacteriophage evasion.
33288623	3	45	attach	present	520:526	arg1	crescentus					534:543	C. crescentus	531:543	C. crescentus	531:543	It is commonly thought that the six paralogs of the flagellin gene present in C. crescentus are important for bacteriophage evasion.
33288623	2	46	theme	bacterial	394:402	arg1	flagellum					404:412	the bacterial flagellum	390:412	the bacterial flagellum	390:412	C. crescentus is a host to many bacteriophages, including ϕCbK and ϕCbK-like bacteriophages, which require interaction with the bacterial flagellum and pilus complexes during adsorption.
33288623	15	47	theme	molecular	2632:2640	arg1	level					2642:2646	the molecular level	2628:2646	the molecular level	2628:2646	We also present a high-resolution structure of a flagellar filament containing only the FljK flagellin, which provides a platform for future studies on determining how bacteriophage ϕCbK attaches to flagellar filaments at the molecular level.
33288623	6	48	theme	deletion	949:956	arg1	strains					958:964	most deletion strains	944:964	most deletion strains	944:964	Interestingly, we observe that most deletion strains still generate flagellar filaments, with the exception of a strain that contains only the most divergent flagellin, FljJ, or a strain that contains only FljN and FljO.
33288623	7	49	theme	FljK	1256:1259	arg1	filament					1261:1268	the FljK filament	1252:1268	the FljK filament	1252:1268	To visualize the surface residues that are likely recognized by ϕCbK, we determined two high-resolution structures of the FljK filament, with and without an amino acid substitution that induces straightening of the filament.
33288623	10	50	theme	filament	1613:1620	arg1	structure					1598:1606	the structure	1594:1606	the structure of a filament composed of a heterogeneous mixture of FljK and FljL, the final resolution of which was limited to approximately 4.6 Å	1594:1739	We also determined the structure of a filament composed of a heterogeneous mixture of FljK and FljL, the final resolution of which was limited to approximately 4.6 Å.
33288623	4	51	theme	specific	617:624	arg1	flagellins					626:635	specific flagellins	617:635	specific flagellins	617:635	Here, we show that deletion of specific flagellins in C. crescentus can indeed attenuate ϕCbK adsorption efficiency, although no single deletion completely ablates ϕCbK adsorption.
33288623	3	52	theme	bacteriophage	563:575	arg1	evasion					577:583	bacteriophage evasion	563:583	bacteriophage evasion	563:583	It is commonly thought that the six paralogs of the flagellin gene present in C. crescentus are important for bacteriophage evasion.
33288623	10	53	theme	heterogeneous	1636:1648	arg1	mixture					1650:1656	a heterogeneous mixture	1634:1656	a heterogeneous mixture	1634:1656	We also determined the structure of a filament composed of a heterogeneous mixture of FljK and FljL, the final resolution of which was limited to approximately 4.6 Å.
33288623	7	54	theme	high-resolution	1222:1236	arg1	structures					1238:1247	two high-resolution structures	1218:1247	two high-resolution structures of the FljK filament	1218:1268	To visualize the surface residues that are likely recognized by ϕCbK, we determined two high-resolution structures of the FljK filament, with and without an amino acid substitution that induces straightening of the filament.
33288623	3	55	from	present	520:526	arg1	crescentus					534:543	C. crescentus	531:543	C. crescentus	531:543	It is commonly thought that the six paralogs of the flagellin gene present in C. crescentus are important for bacteriophage evasion.
33288623	12	56	theme	numerous	2007:2014	arg1	genes					2026:2030	numerous flagellin genes	2007:2030	numerous flagellin genes	2007:2030	Some bacteria harbor numerous flagellin genes and are therefore able to generate flagellar filaments with complex compositions, which is thought to be important for evasion from bacteriophages.
33288623	2	57	theme	ϕCbK-like	333:341	arg1	bacteriophages					343:356	ϕCbK-like bacteriophages	333:356	ϕCbK-like bacteriophages	333:356	C. crescentus is a host to many bacteriophages, including ϕCbK and ϕCbK-like bacteriophages, which require interaction with the bacterial flagellum and pilus complexes during adsorption.
33288623	1	58	theme	fresh-	231:236	arg1	environments					252:263	oligotrophic fresh- and saltwater environments	218:263	oligotrophic fresh- and saltwater environments	218:263	Caulobacter crescentus is a Gram-negative alphaproteobacterium that commonly lives in oligotrophic fresh- and saltwater environments.
33288623	11	59	theme	phage	1815:1819	arg1	ϕCbK					1821:1824	phage ϕCbK	1815:1824	phage ϕCbK	1815:1824	Altogether, this work builds a platform for future investigations of how phage ϕCbK infects C. crescentus at the molecular level.IMPORTANCE Bacterial flagellar filaments serve as an initial attachment point for many bacteriophages to bacteria.
33288623	5	60	theme	various	882:888	arg1	degrees					890:896	various degrees	882:896	various degrees of efficiency	882:910	Thus, the bacteriophage ϕCbK likely recognizes a common motif among the six known flagellins in C. crescentus with various degrees of efficiency.
33288623	4	61	theme	ϕCbK	675:678	arg1	efficiency					691:700	ϕCbK adsorption efficiency	675:700	ϕCbK adsorption efficiency	675:700	Here, we show that deletion of specific flagellins in C. crescentus can indeed attenuate ϕCbK adsorption efficiency, although no single deletion completely ablates ϕCbK adsorption.
33288623	10	62	theme	FljL	1670:1673	arg1	mixture					1650:1656	a heterogeneous mixture	1634:1656	a heterogeneous mixture	1634:1656	We also determined the structure of a filament composed of a heterogeneous mixture of FljK and FljL, the final resolution of which was limited to approximately 4.6 Å.
33288623	15	63	theme	high-resolution	2424:2438	arg1	structure					2440:2448	a high-resolution structure	2422:2448	a high-resolution structure of a flagellar filament containing only the FljK flagellin, which provides a platform for future studies on determining how bacteriophage ϕCbK attaches to flagellar filaments at the molecular level	2422:2646	We also present a high-resolution structure of a flagellar filament containing only the FljK flagellin, which provides a platform for future studies on determining how bacteriophage ϕCbK attaches to flagellar filaments at the molecular level.
33288623	6	64	theme	flagellar	981:989	arg1	filaments					991:999	flagellar filaments	981:999	flagellar filaments	981:999	Interestingly, we observe that most deletion strains still generate flagellar filaments, with the exception of a strain that contains only the most divergent flagellin, FljJ, or a strain that contains only FljN and FljO.
33288623	6	65	contain	contains	1105:1112	arg1	strain					1093:1098	a strain	1091:1098	a strain that contains only FljN and FljO	1091:1131	Interestingly, we observe that most deletion strains still generate flagellar filaments, with the exception of a strain that contains only the most divergent flagellin, FljJ, or a strain that contains only FljN and FljO.
33288623	6	65	contain	contains	1105:1112	arg2	FljO					1128:1131	FljO	1128:1131	FljO	1128:1131	Interestingly, we observe that most deletion strains still generate flagellar filaments, with the exception of a strain that contains only the most divergent flagellin, FljJ, or a strain that contains only FljN and FljO.
33288623	6	65	contain	contains	1105:1112	arg2	FljN					1119:1122	FljN	1119:1122	FljN	1119:1122	Interestingly, we observe that most deletion strains still generate flagellar filaments, with the exception of a strain that contains only the most divergent flagellin, FljJ, or a strain that contains only FljN and FljO.
33288623	4	66	theme	ϕCbK	750:753	arg1	adsorption					755:764	ϕCbK adsorption	750:764	ϕCbK adsorption	750:764	Here, we show that deletion of specific flagellins in C. crescentus can indeed attenuate ϕCbK adsorption efficiency, although no single deletion completely ablates ϕCbK adsorption.
33288623	2	67	theme	many	293:296	arg1	bacteriophages					298:311	many bacteriophages	293:311	many bacteriophages	293:311	C. crescentus is a host to many bacteriophages, including ϕCbK and ϕCbK-like bacteriophages, which require interaction with the bacterial flagellum and pilus complexes during adsorption.
33288623	6	68	theme	divergent	1061:1069	arg1	flagellin					1071:1079	only the most divergent flagellin	1047:1079	only the most divergent flagellin	1047:1079	Interestingly, we observe that most deletion strains still generate flagellar filaments, with the exception of a strain that contains only the most divergent flagellin, FljJ, or a strain that contains only FljN and FljO.
33288623	11	69	theme	molecular	1855:1863	arg1	level.IMPORTANCE					1865:1880	the molecular level.IMPORTANCE	1851:1880	the molecular level.IMPORTANCE Bacterial flagellar filaments serve as an initial attachment point for many bacteriophages to bacteria	1851:1983	Altogether, this work builds a platform for future investigations of how phage ϕCbK infects C. crescentus at the molecular level.IMPORTANCE Bacterial flagellar filaments serve as an initial attachment point for many bacteriophages to bacteria.
33288623	14	70	contain	containing	2324:2333	arg2	flagellin					2344:2352	the FljK flagellin	2335:2352	the FljK flagellin	2335:2352	We find that filaments containing the FljK flagellin are the preferred substrate for bacteriophage ϕCbK.
33288623	14	70	contain	containing	2324:2333	arg1	substrate					2372:2380	the preferred substrate	2358:2380	the preferred substrate for bacteriophage ϕCbK	2358:2403	We find that filaments containing the FljK flagellin are the preferred substrate for bacteriophage ϕCbK.
33288623	14	70	contain	containing	2324:2333	arg1	filaments					2314:2322	filaments	2314:2322	filaments containing the FljK flagellin	2314:2352	We find that filaments containing the FljK flagellin are the preferred substrate for bacteriophage ϕCbK.
33288623	12	71	theme	complex	2092:2098	arg1	compositions					2100:2111	complex compositions	2092:2111	complex compositions	2092:2111	Some bacteria harbor numerous flagellin genes and are therefore able to generate flagellar filaments with complex compositions, which is thought to be important for evasion from bacteriophages.
33288623	14	72	theme	preferred	2362:2370	arg1	filaments					2314:2322	filaments	2314:2322	filaments containing the FljK flagellin	2314:2352	We find that filaments containing the FljK flagellin are the preferred substrate for bacteriophage ϕCbK.
33288623	14	72	theme	preferred	2362:2370	arg1	substrate					2372:2380	the preferred substrate	2358:2380	the preferred substrate for bacteriophage ϕCbK	2358:2403	We find that filaments containing the FljK flagellin are the preferred substrate for bacteriophage ϕCbK.
33288623	0	73	theme	CbK	90:92	arg1	Predation					94:102	Phage CbK Predation	84:102	Phage CbK Predation of Multiflagellin Bacteria	84:129	Flagellar Structures from the Bacterium Caulobacter crescentus and Implications for Phage CbK Predation of Multiflagellin Bacteria.
33288623	15	74	theme	flagellar	2605:2613	arg1	filaments					2615:2623	flagellar filaments	2605:2623	flagellar filaments at the molecular level	2605:2646	We also present a high-resolution structure of a flagellar filament containing only the FljK flagellin, which provides a platform for future studies on determining how bacteriophage ϕCbK attaches to flagellar filaments at the molecular level.
33288623	6	75	theme	strain	1026:1031	arg1	exception					1011:1019	the exception	1007:1019	the exception of a strain that contains only the most divergent flagellin, FljJ, or a strain that contains only FljN and FljO	1007:1131	Interestingly, we observe that most deletion strains still generate flagellar filaments, with the exception of a strain that contains only the most divergent flagellin, FljJ, or a strain that contains only FljN and FljO.
33288623	14	76	theme	FljK	2339:2342	arg1	flagellin					2344:2352	the FljK flagellin	2335:2352	the FljK flagellin	2335:2352	We find that filaments containing the FljK flagellin are the preferred substrate for bacteriophage ϕCbK.
33288623	11	77	theme	future	1786:1791	arg1	investigations					1793:1806	future investigations	1786:1806	future investigations of how phage ϕCbK infects C. crescentus at the molecular level.IMPORTANCE Bacterial flagellar filaments serve as an initial attachment point for many bacteriophages to bacteria	1786:1983	Altogether, this work builds a platform for future investigations of how phage ϕCbK infects C. crescentus at the molecular level.IMPORTANCE Bacterial flagellar filaments serve as an initial attachment point for many bacteriophages to bacteria.
33288623	0	78	theme	Bacteria	122:129	arg1	Predation					94:102	Phage CbK Predation	84:102	Phage CbK Predation of Multiflagellin Bacteria	84:129	Flagellar Structures from the Bacterium Caulobacter crescentus and Implications for Phage CbK Predation of Multiflagellin Bacteria.
33288623	12	79	from	bacteriophages	2164:2177	arg1	evasion					2151:2157	evasion	2151:2157	evasion from bacteriophages	2151:2177	Some bacteria harbor numerous flagellin genes and are therefore able to generate flagellar filaments with complex compositions, which is thought to be important for evasion from bacteriophages.
33288623	5	80	theme	bacteriophage	777:789	arg1	ϕCbK					791:794	the bacteriophage ϕCbK	773:794	the bacteriophage ϕCbK	773:794	Thus, the bacteriophage ϕCbK likely recognizes a common motif among the six known flagellins in C. crescentus with various degrees of efficiency.
33288623	5	81	theme	known	843:847	arg1	flagellins					849:858	the six known flagellins	835:858	the six known flagellins	835:858	Thus, the bacteriophage ϕCbK likely recognizes a common motif among the six known flagellins in C. crescentus with various degrees of efficiency.
33288623	15	82	theme	flagellar	2455:2463	arg1	filament					2465:2472	a flagellar filament	2453:2472	a flagellar filament containing only the FljK flagellin, which provides a platform for future studies on determining how bacteriophage ϕCbK attaches to flagellar filaments at the molecular level	2453:2646	We also present a high-resolution structure of a flagellar filament containing only the FljK flagellin, which provides a platform for future studies on determining how bacteriophage ϕCbK attaches to flagellar filaments at the molecular level.
33288623	15	83	theme	bacteriophage	2574:2586	arg1	ϕCbK					2588:2591	bacteriophage ϕCbK	2574:2591	bacteriophage ϕCbK	2574:2591	We also present a high-resolution structure of a flagellar filament containing only the FljK flagellin, which provides a platform for future studies on determining how bacteriophage ϕCbK attaches to flagellar filaments at the molecular level.
33288623	8	84	theme	posttranslational	1370:1386	arg1	modifications					1388:1400	posttranslational modifications	1370:1400	posttranslational modifications on conserved surface threonine residues of FljK that are likely O-linked glycans	1370:1481	We observe posttranslational modifications on conserved surface threonine residues of FljK that are likely O-linked glycans.
33288623	8	84	theme	posttranslational	1370:1386	arg1	glycans					1475:1481	likely O-linked glycans	1459:1481	likely O-linked glycans	1459:1481	We observe posttranslational modifications on conserved surface threonine residues of FljK that are likely O-linked glycans.
33288623	11	85	theme	attachment	1932:1941	arg1	point					1943:1947	an initial attachment point	1921:1947	an initial attachment point for many bacteriophages to bacteria	1921:1983	Altogether, this work builds a platform for future investigations of how phage ϕCbK infects C. crescentus at the molecular level.IMPORTANCE Bacterial flagellar filaments serve as an initial attachment point for many bacteriophages to bacteria.
33288623	11	85	theme	attachment	1932:1941	arg1	filaments					1902:1910	Bacterial flagellar filaments	1882:1910	Bacterial flagellar filaments	1882:1910	Altogether, this work builds a platform for future investigations of how phage ϕCbK infects C. crescentus at the molecular level.IMPORTANCE Bacterial flagellar filaments serve as an initial attachment point for many bacteriophages to bacteria.
33288623	6	86	contain	contains	1038:1045	arg1	FljJ					1082:1085	FljJ	1082:1085	FljJ	1082:1085	Interestingly, we observe that most deletion strains still generate flagellar filaments, with the exception of a strain that contains only the most divergent flagellin, FljJ, or a strain that contains only FljN and FljO.
33288623	6	86	contain	contains	1038:1045	arg2	flagellin					1071:1079	only the most divergent flagellin	1047:1079	only the most divergent flagellin	1047:1079	Interestingly, we observe that most deletion strains still generate flagellar filaments, with the exception of a strain that contains only the most divergent flagellin, FljJ, or a strain that contains only FljN and FljO.
33288623	6	86	contain	contains	1038:1045	arg1	strain					1026:1031	a strain	1024:1031	a strain that contains only the most divergent flagellin	1024:1079	Interestingly, we observe that most deletion strains still generate flagellar filaments, with the exception of a strain that contains only the most divergent flagellin, FljJ, or a strain that contains only FljN and FljO.
33288623	3	87	theme	flagellin	505:513	arg1	gene					515:518	the flagellin gene	501:518	the flagellin gene present in C. crescentus	501:543	It is commonly thought that the six paralogs of the flagellin gene present in C. crescentus are important for bacteriophage evasion.
33288623	0	88	theme	Caulobacter	40:50	arg1	crescentus					52:61	the Bacterium Caulobacter crescentus	26:61	the Bacterium Caulobacter crescentus	26:61	Flagellar Structures from the Bacterium Caulobacter crescentus and Implications for Phage CbK Predation of Multiflagellin Bacteria.
33288623	1	89	theme	saltwater	242:250	arg1	environments					252:263	oligotrophic fresh- and saltwater environments	218:263	oligotrophic fresh- and saltwater environments	218:263	Caulobacter crescentus is a Gram-negative alphaproteobacterium that commonly lives in oligotrophic fresh- and saltwater environments.
33288623	7	90	theme	amino	1291:1295	arg1	substitution					1302:1313	an amino acid substitution	1288:1313	an amino acid substitution that induces straightening of the filament	1288:1356	To visualize the surface residues that are likely recognized by ϕCbK, we determined two high-resolution structures of the FljK filament, with and without an amino acid substitution that induces straightening of the filament.
33288623	8	91	theme	surface	1415:1421	arg1	residues					1433:1440	conserved surface threonine residues	1405:1440	conserved surface threonine residues of FljK	1405:1448	We observe posttranslational modifications on conserved surface threonine residues of FljK that are likely O-linked glycans.
33288623	8	91	theme	surface	1415:1421	arg1	FljK					1445:1448	FljK	1445:1448	FljK	1445:1448	We observe posttranslational modifications on conserved surface threonine residues of FljK that are likely O-linked glycans.
33288623	0	92	theme	Multiflagellin	107:120	arg1	Bacteria					122:129	Multiflagellin Bacteria	107:129	Multiflagellin Bacteria	107:129	Flagellar Structures from the Bacterium Caulobacter crescentus and Implications for Phage CbK Predation of Multiflagellin Bacteria.
33288623	2	93	with	interaction	373:383	arg1	flagellum					404:412	the bacterial flagellum	390:412	the bacterial flagellum	390:412	C. crescentus is a host to many bacteriophages, including ϕCbK and ϕCbK-like bacteriophages, which require interaction with the bacterial flagellum and pilus complexes during adsorption.
33288623	9	94	theme	interplay	1503:1511	arg1	possibility					1488:1498	The possibility	1484:1498	The possibility of interplay between these modifications and ϕCbK adsorption	1484:1559	The possibility of interplay between these modifications and ϕCbK adsorption is discussed.
33288623	11	95	theme	flagellar	1892:1900	arg1	point					1943:1947	an initial attachment point	1921:1947	an initial attachment point for many bacteriophages to bacteria	1921:1983	Altogether, this work builds a platform for future investigations of how phage ϕCbK infects C. crescentus at the molecular level.IMPORTANCE Bacterial flagellar filaments serve as an initial attachment point for many bacteriophages to bacteria.
33288623	11	95	theme	flagellar	1892:1900	arg1	filaments					1902:1910	Bacterial flagellar filaments	1882:1910	Bacterial flagellar filaments	1882:1910	Altogether, this work builds a platform for future investigations of how phage ϕCbK infects C. crescentus at the molecular level.IMPORTANCE Bacterial flagellar filaments serve as an initial attachment point for many bacteriophages to bacteria.
33288623	15	96	contain	containing	2474:2483	arg1	filament					2465:2472	a flagellar filament	2453:2472	a flagellar filament containing only the FljK flagellin, which provides a platform for future studies on determining how bacteriophage ϕCbK attaches to flagellar filaments at the molecular level	2453:2646	We also present a high-resolution structure of a flagellar filament containing only the FljK flagellin, which provides a platform for future studies on determining how bacteriophage ϕCbK attaches to flagellar filaments at the molecular level.
33288623	15	96	contain	containing	2474:2483	arg2	flagellin					2499:2507	only the FljK flagellin	2485:2507	only the FljK flagellin	2485:2507	We also present a high-resolution structure of a flagellar filament containing only the FljK flagellin, which provides a platform for future studies on determining how bacteriophage ϕCbK attaches to flagellar filaments at the molecular level.
33288623	8	97	theme	FljK	1445:1448	arg1	residues					1433:1440	conserved surface threonine residues	1405:1440	conserved surface threonine residues of FljK	1405:1448	We observe posttranslational modifications on conserved surface threonine residues of FljK that are likely O-linked glycans.
33288623	8	97	theme	FljK	1445:1448	arg1	FljK					1445:1448	FljK	1445:1448	FljK	1445:1448	We observe posttranslational modifications on conserved surface threonine residues of FljK that are likely O-linked glycans.
33288623	3	98	theme	present	520:526	arg1	gene					515:518	the flagellin gene	501:518	the flagellin gene present in C. crescentus	501:543	It is commonly thought that the six paralogs of the flagellin gene present in C. crescentus are important for bacteriophage evasion.
33288623	12	99	theme	flagellar	2067:2075	arg1	filaments					2077:2085	flagellar filaments	2067:2085	flagellar filaments	2067:2085	Some bacteria harbor numerous flagellin genes and are therefore able to generate flagellar filaments with complex compositions, which is thought to be important for evasion from bacteriophages.
33288623	7	100	theme	filament	1261:1268	arg1	structures					1238:1247	two high-resolution structures	1218:1247	two high-resolution structures of the FljK filament	1218:1268	To visualize the surface residues that are likely recognized by ϕCbK, we determined two high-resolution structures of the FljK filament, with and without an amino acid substitution that induces straightening of the filament.
33288623	13	101	theme	genes	2241:2245	arg1	importance					2209:2218	the importance	2205:2218	the importance of the six flagellin genes in C. crescentus for infection by bacteriophage ϕCbK	2205:2298	This study characterizes the importance of the six flagellin genes in C. crescentus for infection by bacteriophage ϕCbK.
31463900	0	0	theme	Cell-Based	78:87	arg1	Assays					89:94	Cell-Based Assays	78:94	Cell-Based Assays	78:94	Analyzing the Effects of O-Fucosylation on Secretion of ADAMTS Proteins Using Cell-Based Assays.
31463900	0	1	from	Effects	14:20	arg1	Secretion					43:51	Secretion	43:51	Secretion of ADAMTS Proteins	43:70	Analyzing the Effects of O-Fucosylation on Secretion of ADAMTS Proteins Using Cell-Based Assays.
31463900	1	2	with	metalloproteinase	149:165	arg1	repeats					194:200	thrombospondin type 1 repeats	172:200	thrombospondin type 1 repeats	172:200	Metalloproteinases of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin type 1 repeats) superfamily are extensively modified with glycan moieties.
31463900	4	3	from	lethality	792:800	arg1	mice					805:808	mice	805:808	mice	805:808	Knockout of POFUT2 results in embryonic lethality in mice, and inactivating mutations in B3GLCT cause Peters plus syndrome, a congenital disorder of glycosylation in humans.
31463900	3	4	theme	O-linked	525:532	arg1	fucose					534:539	O-linked fucose	525:539	O-linked fucose	525:539	This chapter focuses on O-linked fucose, which is added to properly folded thrombospondin type 1 repeats (TSRs) in the ER by protein O-fucosyltransferase 2 (POFUT2) and elongated to a Glucoseβ1-3Fucose disaccharide by β3-glucosyltransferase (B3GLCT).
31463900	6	5	theme	quality	1139:1145	arg1	pathway					1155:1161	an ER quality control pathway	1133:1161	an ER quality control pathway for folding of TSRs in ADAMTS proteins	1133:1200	Thus, POFUT2 and B3GLCT both function as an ER quality control pathway for folding of TSRs in ADAMTS proteins.
31463900	6	5	theme	quality	1139:1145	arg1	POFUT2					1098:1103	POFUT2	1098:1103	POFUT2	1098:1103	Thus, POFUT2 and B3GLCT both function as an ER quality control pathway for folding of TSRs in ADAMTS proteins.
31463900	6	5	theme	quality	1139:1145	arg1	B3GLCT					1109:1114	B3GLCT	1109:1114	B3GLCT	1109:1114	Thus, POFUT2 and B3GLCT both function as an ER quality control pathway for folding of TSRs in ADAMTS proteins.
31463900	2	6	theme	extracellular	394:406	arg1	space					408:412	the extracellular space	390:412	the extracellular space	390:412	Glycosylation occurs as these enzymes are trafficked through the endoplasmic reticulum (ER) and Golgi apparatus on their way to the extracellular space and includes N-linked glycosylation, O-linked fucosylation and C-linked mannosylation.
31463900	2	7	theme	Golgi	358:362	arg1	apparatus					364:372	Golgi apparatus	358:372	Golgi apparatus	358:372	Glycosylation occurs as these enzymes are trafficked through the endoplasmic reticulum (ER) and Golgi apparatus on their way to the extracellular space and includes N-linked glycosylation, O-linked fucosylation and C-linked mannosylation.
31463900	5	8	theme	folded	987:992	arg1	TSRs					994:997	folded TSRs	987:997	folded TSRs	987:997	Addition of the disaccharide by POFUT2 and B3GLCT stabilizes folded TSRs, enhancing folding in the ER and secretion efficiency of several ADAMTS proteins from cells.
31463900	7	9	theme	proteins	1302:1309	arg1	defects					1284:1290	secretion defects	1274:1290	secretion defects of ADAMTS proteins	1274:1309	In this chapter we describe in detail the methods developed to analyze secretion defects of ADAMTS proteins upon loss of either POFUT2 or B3GLCT.
31463900	1	10	with	disintegrin	133:143	arg1	repeats					194:200	thrombospondin type 1 repeats	172:200	thrombospondin type 1 repeats	172:200	Metalloproteinases of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin type 1 repeats) superfamily are extensively modified with glycan moieties.
31463900	6	11	theme	control	1147:1153	arg1	pathway					1155:1161	an ER quality control pathway	1133:1161	an ER quality control pathway for folding of TSRs in ADAMTS proteins	1133:1200	Thus, POFUT2 and B3GLCT both function as an ER quality control pathway for folding of TSRs in ADAMTS proteins.
31463900	6	11	theme	control	1147:1153	arg1	POFUT2					1098:1103	POFUT2	1098:1103	POFUT2	1098:1103	Thus, POFUT2 and B3GLCT both function as an ER quality control pathway for folding of TSRs in ADAMTS proteins.
31463900	6	11	theme	control	1147:1153	arg1	B3GLCT					1109:1114	B3GLCT	1109:1114	B3GLCT	1109:1114	Thus, POFUT2 and B3GLCT both function as an ER quality control pathway for folding of TSRs in ADAMTS proteins.
31463900	8	12	theme	knockout	1412:1419	arg1	lines					1426:1430	CRISPR/Cas9-mediated knockout cell lines	1391:1430	CRISPR/Cas9-mediated knockout cell lines	1391:1430	The methods described include creation of CRISPR/Cas9-mediated knockout cell lines of POFUT2 and B3GLCT and use of these cell lines to analyze and quantify secretion defects of ADAMTS proteins.
31463900	5	13	from	cells	1085:1089	arg1	proteins					1071:1078	several ADAMTS proteins	1056:1078	several ADAMTS proteins from cells	1056:1089	Addition of the disaccharide by POFUT2 and B3GLCT stabilizes folded TSRs, enhancing folding in the ER and secretion efficiency of several ADAMTS proteins from cells.
31463900	5	13	from	cells	1085:1089	arg1	efficiency					1042:1051	folding in the ER and secretion efficiency	1010:1051	folding in the ER and secretion efficiency of several ADAMTS proteins from cells	1010:1089	Addition of the disaccharide by POFUT2 and B3GLCT stabilizes folded TSRs, enhancing folding in the ER and secretion efficiency of several ADAMTS proteins from cells.
31463900	6	14	theme	ER	1136:1137	arg1	pathway					1155:1161	an ER quality control pathway	1133:1161	an ER quality control pathway for folding of TSRs in ADAMTS proteins	1133:1200	Thus, POFUT2 and B3GLCT both function as an ER quality control pathway for folding of TSRs in ADAMTS proteins.
31463900	6	14	theme	ER	1136:1137	arg1	POFUT2					1098:1103	POFUT2	1098:1103	POFUT2	1098:1103	Thus, POFUT2 and B3GLCT both function as an ER quality control pathway for folding of TSRs in ADAMTS proteins.
31463900	6	14	theme	ER	1136:1137	arg1	B3GLCT					1109:1114	B3GLCT	1109:1114	B3GLCT	1109:1114	Thus, POFUT2 and B3GLCT both function as an ER quality control pathway for folding of TSRs in ADAMTS proteins.
31463900	4	15	theme	POFUT2	764:769	arg1	Knockout					752:759	Knockout	752:759	Knockout	752:759	Knockout of POFUT2 results in embryonic lethality in mice, and inactivating mutations in B3GLCT cause Peters plus syndrome, a congenital disorder of glycosylation in humans.
31463900	4	16	gly	glycosylation	901:913	arg1	humans					918:923	humans	918:923	humans	918:923	Knockout of POFUT2 results in embryonic lethality in mice, and inactivating mutations in B3GLCT cause Peters plus syndrome, a congenital disorder of glycosylation in humans.
31463900	5	17	from	secretion	1032:1040	arg1	ER					1025:1026	the ER	1021:1026	the ER	1021:1026	Addition of the disaccharide by POFUT2 and B3GLCT stabilizes folded TSRs, enhancing folding in the ER and secretion efficiency of several ADAMTS proteins from cells.
31463900	8	18	theme	ADAMTS	1526:1531	arg1	proteins					1533:1540	ADAMTS proteins	1526:1540	ADAMTS proteins	1526:1540	The methods described include creation of CRISPR/Cas9-mediated knockout cell lines of POFUT2 and B3GLCT and use of these cell lines to analyze and quantify secretion defects of ADAMTS proteins.
31463900	2	19	link	O-linked	451:458	arg1	fucosylation					460:471	O-linked fucosylation	451:471	O-linked fucosylation	451:471	Glycosylation occurs as these enzymes are trafficked through the endoplasmic reticulum (ER) and Golgi apparatus on their way to the extracellular space and includes N-linked glycosylation, O-linked fucosylation and C-linked mannosylation.
31463900	4	20	theme	embryonic	782:790	arg1	lethality					792:800	embryonic lethality	782:800	embryonic lethality in mice	782:808	Knockout of POFUT2 results in embryonic lethality in mice, and inactivating mutations in B3GLCT cause Peters plus syndrome, a congenital disorder of glycosylation in humans.
31463900	5	21	from	folding	1010:1016	arg1	ER					1025:1026	the ER	1021:1026	the ER	1021:1026	Addition of the disaccharide by POFUT2 and B3GLCT stabilizes folded TSRs, enhancing folding in the ER and secretion efficiency of several ADAMTS proteins from cells.
31463900	1	22	theme	thrombospondin	172:185	arg1	repeats					194:200	thrombospondin type 1 repeats	172:200	thrombospondin type 1 repeats	172:200	Metalloproteinases of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin type 1 repeats) superfamily are extensively modified with glycan moieties.
31463900	8	23	theme	secretion	1505:1513	arg1	defects					1515:1521	secretion defects	1505:1521	secretion defects of ADAMTS proteins	1505:1540	The methods described include creation of CRISPR/Cas9-mediated knockout cell lines of POFUT2 and B3GLCT and use of these cell lines to analyze and quantify secretion defects of ADAMTS proteins.
31463900	2	24	link	C-linked	477:484	arg1	mannosylation					486:498	C-linked mannosylation	477:498	C-linked mannosylation	477:498	Glycosylation occurs as these enzymes are trafficked through the endoplasmic reticulum (ER) and Golgi apparatus on their way to the extracellular space and includes N-linked glycosylation, O-linked fucosylation and C-linked mannosylation.
31463900	1	25	dep	ADAMTS	123:128	arg1	metalloproteinase					149:165	metalloproteinase	149:165	metalloproteinase	149:165	Metalloproteinases of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin type 1 repeats) superfamily are extensively modified with glycan moieties.
31463900	1	25	dep	ADAMTS	123:128	arg1	ADAMTS					123:128	ADAMTS	123:128	the ADAMTS (a disintegrin and metalloproteinase with thrombospondin type 1 repeats) superfamily	119:213	Metalloproteinases of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin type 1 repeats) superfamily are extensively modified with glycan moieties.
31463900	1	25	dep	ADAMTS	123:128	arg1	disintegrin					133:143	a disintegrin	131:143	a disintegrin	131:143	Metalloproteinases of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin type 1 repeats) superfamily are extensively modified with glycan moieties.
31463900	6	26	from	folding	1167:1173	arg1	proteins					1193:1200	ADAMTS proteins	1186:1200	ADAMTS proteins	1186:1200	Thus, POFUT2 and B3GLCT both function as an ER quality control pathway for folding of TSRs in ADAMTS proteins.
31463900	1	27	theme	type	187:190	arg1	repeats					194:200	thrombospondin type 1 repeats	172:200	thrombospondin type 1 repeats	172:200	Metalloproteinases of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin type 1 repeats) superfamily are extensively modified with glycan moieties.
31463900	5	28	from	efficiency	1042:1051	arg1	cells					1085:1089	cells	1085:1089	cells	1085:1089	Addition of the disaccharide by POFUT2 and B3GLCT stabilizes folded TSRs, enhancing folding in the ER and secretion efficiency of several ADAMTS proteins from cells.
31463900	3	29	theme	type	591:594	arg1	TSRs					607:610	TSRs	607:610	TSRs	607:610	This chapter focuses on O-linked fucose, which is added to properly folded thrombospondin type 1 repeats (TSRs) in the ER by protein O-fucosyltransferase 2 (POFUT2) and elongated to a Glucoseβ1-3Fucose disaccharide by β3-glucosyltransferase (B3GLCT).
31463900	3	29	theme	type	591:594	arg1	repeats					598:604	properly folded thrombospondin type 1 repeats	560:604	properly folded thrombospondin type 1 repeats (TSRs) in the ER	560:621	This chapter focuses on O-linked fucose, which is added to properly folded thrombospondin type 1 repeats (TSRs) in the ER by protein O-fucosyltransferase 2 (POFUT2) and elongated to a Glucoseβ1-3Fucose disaccharide by β3-glucosyltransferase (B3GLCT).
31463900	2	30	theme	O-linked	451:458	arg1	fucosylation					460:471	O-linked fucosylation	451:471	O-linked fucosylation	451:471	Glycosylation occurs as these enzymes are trafficked through the endoplasmic reticulum (ER) and Golgi apparatus on their way to the extracellular space and includes N-linked glycosylation, O-linked fucosylation and C-linked mannosylation.
31463900	5	31	theme	several	1056:1062	arg1	proteins					1071:1078	several ADAMTS proteins	1056:1078	several ADAMTS proteins from cells	1056:1089	Addition of the disaccharide by POFUT2 and B3GLCT stabilizes folded TSRs, enhancing folding in the ER and secretion efficiency of several ADAMTS proteins from cells.
31463900	2	32	theme	endoplasmic	327:337	arg1	ER					350:351	ER	350:351	ER	350:351	Glycosylation occurs as these enzymes are trafficked through the endoplasmic reticulum (ER) and Golgi apparatus on their way to the extracellular space and includes N-linked glycosylation, O-linked fucosylation and C-linked mannosylation.
31463900	2	32	theme	endoplasmic	327:337	arg1	reticulum					339:347	the endoplasmic reticulum	323:347	the endoplasmic reticulum (ER)	323:352	Glycosylation occurs as these enzymes are trafficked through the endoplasmic reticulum (ER) and Golgi apparatus on their way to the extracellular space and includes N-linked glycosylation, O-linked fucosylation and C-linked mannosylation.
31463900	3	33	from	repeats	598:604	arg1	ER					620:621	the ER	616:621	the ER	616:621	This chapter focuses on O-linked fucose, which is added to properly folded thrombospondin type 1 repeats (TSRs) in the ER by protein O-fucosyltransferase 2 (POFUT2) and elongated to a Glucoseβ1-3Fucose disaccharide by β3-glucosyltransferase (B3GLCT).
31463900	7	34	theme	ADAMTS	1295:1300	arg1	proteins					1302:1309	ADAMTS proteins	1295:1309	ADAMTS proteins	1295:1309	In this chapter we describe in detail the methods developed to analyze secretion defects of ADAMTS proteins upon loss of either POFUT2 or B3GLCT.
31463900	6	35	dep	function	1121:1128	arg1	both					1116:1119	both	1116:1119	both	1116:1119	Thus, POFUT2 and B3GLCT both function as an ER quality control pathway for folding of TSRs in ADAMTS proteins.
31463900	3	36	link	O-linked	525:532	arg1	fucose					534:539	O-linked fucose	525:539	O-linked fucose	525:539	This chapter focuses on O-linked fucose, which is added to properly folded thrombospondin type 1 repeats (TSRs) in the ER by protein O-fucosyltransferase 2 (POFUT2) and elongated to a Glucoseβ1-3Fucose disaccharide by β3-glucosyltransferase (B3GLCT).
31463900	3	37	theme	folded	569:574	arg1	TSRs					607:610	TSRs	607:610	TSRs	607:610	This chapter focuses on O-linked fucose, which is added to properly folded thrombospondin type 1 repeats (TSRs) in the ER by protein O-fucosyltransferase 2 (POFUT2) and elongated to a Glucoseβ1-3Fucose disaccharide by β3-glucosyltransferase (B3GLCT).
31463900	3	37	theme	folded	569:574	arg1	repeats					598:604	properly folded thrombospondin type 1 repeats	560:604	properly folded thrombospondin type 1 repeats (TSRs) in the ER	560:621	This chapter focuses on O-linked fucose, which is added to properly folded thrombospondin type 1 repeats (TSRs) in the ER by protein O-fucosyltransferase 2 (POFUT2) and elongated to a Glucoseβ1-3Fucose disaccharide by β3-glucosyltransferase (B3GLCT).
31463900	2	38	theme	N-linked	427:434	arg1	glycosylation					436:448	N-linked glycosylation	427:448	N-linked glycosylation	427:448	Glycosylation occurs as these enzymes are trafficked through the endoplasmic reticulum (ER) and Golgi apparatus on their way to the extracellular space and includes N-linked glycosylation, O-linked fucosylation and C-linked mannosylation.
31463900	5	39	theme	disaccharide	942:953	arg1	Addition					926:933	Addition	926:933	Addition of the disaccharide by POFUT2 and B3GLCT	926:974	Addition of the disaccharide by POFUT2 and B3GLCT stabilizes folded TSRs, enhancing folding in the ER and secretion efficiency of several ADAMTS proteins from cells.
31463900	8	40	theme	proteins	1533:1540	arg1	defects					1515:1521	secretion defects	1505:1521	secretion defects of ADAMTS proteins	1505:1540	The methods described include creation of CRISPR/Cas9-mediated knockout cell lines of POFUT2 and B3GLCT and use of these cell lines to analyze and quantify secretion defects of ADAMTS proteins.
31463900	8	41	theme	cell	1421:1424	arg1	lines					1426:1430	CRISPR/Cas9-mediated knockout cell lines	1391:1430	CRISPR/Cas9-mediated knockout cell lines	1391:1430	The methods described include creation of CRISPR/Cas9-mediated knockout cell lines of POFUT2 and B3GLCT and use of these cell lines to analyze and quantify secretion defects of ADAMTS proteins.
31463900	5	42	theme	ADAMTS	1064:1069	arg1	proteins					1071:1078	several ADAMTS proteins	1056:1078	several ADAMTS proteins from cells	1056:1089	Addition of the disaccharide by POFUT2 and B3GLCT stabilizes folded TSRs, enhancing folding in the ER and secretion efficiency of several ADAMTS proteins from cells.
31463900	3	43	theme	thrombospondin	576:589	arg1	type					591:594	thrombospondin type 1	576:596	properly folded thrombospondin type 1 repeats (TSRs) in the ER	560:621	This chapter focuses on O-linked fucose, which is added to properly folded thrombospondin type 1 repeats (TSRs) in the ER by protein O-fucosyltransferase 2 (POFUT2) and elongated to a Glucoseβ1-3Fucose disaccharide by β3-glucosyltransferase (B3GLCT).
31463900	1	44	theme	superfamily	203:213	arg1	Metalloproteinases					97:114	Metalloproteinases	97:114	Metalloproteinases of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin type 1 repeats) superfamily	97:213	Metalloproteinases of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin type 1 repeats) superfamily are extensively modified with glycan moieties.
31463900	1	45	mod	modified	231:238	arg1	Metalloproteinases					97:114	Metalloproteinases	97:114	Metalloproteinases of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin type 1 repeats) superfamily	97:213	Metalloproteinases of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin type 1 repeats) superfamily are extensively modified with glycan moieties.
31463900	1	45	mod	modified	231:238	arg3	moieties					252:259	glycan moieties	245:259	glycan moieties	245:259	Metalloproteinases of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin type 1 repeats) superfamily are extensively modified with glycan moieties.
31463900	5	46	theme	proteins	1071:1078	arg1	efficiency					1042:1051	folding in the ER and secretion efficiency	1010:1051	folding in the ER and secretion efficiency of several ADAMTS proteins from cells	1010:1089	Addition of the disaccharide by POFUT2 and B3GLCT stabilizes folded TSRs, enhancing folding in the ER and secretion efficiency of several ADAMTS proteins from cells.
31463900	4	47	theme	inactivating	815:826	arg1	mutations					828:836	inactivating mutations	815:836	inactivating mutations in B3GLCT	815:846	Knockout of POFUT2 results in embryonic lethality in mice, and inactivating mutations in B3GLCT cause Peters plus syndrome, a congenital disorder of glycosylation in humans.
31463900	0	48	theme	O-Fucosylation	25:38	arg1	Effects					14:20	the Effects	10:20	the Effects of O-Fucosylation on Secretion of ADAMTS Proteins	10:70	Analyzing the Effects of O-Fucosylation on Secretion of ADAMTS Proteins Using Cell-Based Assays.
31463900	3	49	theme	protein	626:632	arg1	O-fucosyltransferase					634:653	protein O-fucosyltransferase 2	626:655	protein O-fucosyltransferase 2 (POFUT2)	626:664	This chapter focuses on O-linked fucose, which is added to properly folded thrombospondin type 1 repeats (TSRs) in the ER by protein O-fucosyltransferase 2 (POFUT2) and elongated to a Glucoseβ1-3Fucose disaccharide by β3-glucosyltransferase (B3GLCT).
31463900	3	49	theme	protein	626:632	arg1	POFUT2					658:663	POFUT2	658:663	POFUT2	658:663	This chapter focuses on O-linked fucose, which is added to properly folded thrombospondin type 1 repeats (TSRs) in the ER by protein O-fucosyltransferase 2 (POFUT2) and elongated to a Glucoseβ1-3Fucose disaccharide by β3-glucosyltransferase (B3GLCT).
31463900	2	50	theme	C-linked	477:484	arg1	mannosylation					486:498	C-linked mannosylation	477:498	C-linked mannosylation	477:498	Glycosylation occurs as these enzymes are trafficked through the endoplasmic reticulum (ER) and Golgi apparatus on their way to the extracellular space and includes N-linked glycosylation, O-linked fucosylation and C-linked mannosylation.
31463900	4	51	theme	congenital	878:887	arg1	Peters					854:859	Peters plus syndrome	854:873	Peters	854:859	Knockout of POFUT2 results in embryonic lethality in mice, and inactivating mutations in B3GLCT cause Peters plus syndrome, a congenital disorder of glycosylation in humans.
31463900	4	51	theme	congenital	878:887	arg1	disorder					889:896	a congenital disorder	876:896	a congenital disorder of glycosylation in humans	876:923	Knockout of POFUT2 results in embryonic lethality in mice, and inactivating mutations in B3GLCT cause Peters plus syndrome, a congenital disorder of glycosylation in humans.
31463900	4	52	from	humans	918:923	arg1	Peters					854:859	Peters plus syndrome	854:873	Peters	854:859	Knockout of POFUT2 results in embryonic lethality in mice, and inactivating mutations in B3GLCT cause Peters plus syndrome, a congenital disorder of glycosylation in humans.
31463900	4	52	from	humans	918:923	arg1	disorder					889:896	a congenital disorder	876:896	a congenital disorder of glycosylation in humans	876:923	Knockout of POFUT2 results in embryonic lethality in mice, and inactivating mutations in B3GLCT cause Peters plus syndrome, a congenital disorder of glycosylation in humans.
31463900	4	53	from	mutations	828:836	arg1	B3GLCT					841:846	B3GLCT	841:846	B3GLCT	841:846	Knockout of POFUT2 results in embryonic lethality in mice, and inactivating mutations in B3GLCT cause Peters plus syndrome, a congenital disorder of glycosylation in humans.
31463900	8	54	theme	lines	1475:1479	arg1	creation					1379:1386	creation	1379:1386	creation of CRISPR/Cas9-mediated knockout cell lines of POFUT2 and B3GLCT	1379:1451	The methods described include creation of CRISPR/Cas9-mediated knockout cell lines of POFUT2 and B3GLCT and use of these cell lines to analyze and quantify secretion defects of ADAMTS proteins.
31463900	8	54	theme	lines	1475:1479	arg1	use					1457:1459	use	1457:1459	use of these cell lines to analyze and quantify secretion defects of ADAMTS proteins	1457:1540	The methods described include creation of CRISPR/Cas9-mediated knockout cell lines of POFUT2 and B3GLCT and use of these cell lines to analyze and quantify secretion defects of ADAMTS proteins.
31463900	8	55	theme	cell	1470:1473	arg1	lines					1475:1479	these cell lines	1464:1479	these cell lines	1464:1479	The methods described include creation of CRISPR/Cas9-mediated knockout cell lines of POFUT2 and B3GLCT and use of these cell lines to analyze and quantify secretion defects of ADAMTS proteins.
31463900	5	56	theme	secretion	1032:1040	arg1	efficiency					1042:1051	folding in the ER and secretion efficiency	1010:1051	folding in the ER and secretion efficiency of several ADAMTS proteins from cells	1010:1089	Addition of the disaccharide by POFUT2 and B3GLCT stabilizes folded TSRs, enhancing folding in the ER and secretion efficiency of several ADAMTS proteins from cells.
31463900	7	57	theme	B3GLCT	1341:1346	arg1	loss					1316:1319	loss	1316:1319	loss of either POFUT2 or B3GLCT	1316:1346	In this chapter we describe in detail the methods developed to analyze secretion defects of ADAMTS proteins upon loss of either POFUT2 or B3GLCT.
31463900	1	58	theme	ADAMTS	123:128	arg1	superfamily					203:213	the ADAMTS (a disintegrin and metalloproteinase with thrombospondin type 1 repeats) superfamily	119:213	the ADAMTS (a disintegrin and metalloproteinase with thrombospondin type 1 repeats) superfamily	119:213	Metalloproteinases of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin type 1 repeats) superfamily are extensively modified with glycan moieties.
31463900	8	59	theme	lines	1426:1430	arg1	creation					1379:1386	creation	1379:1386	creation of CRISPR/Cas9-mediated knockout cell lines of POFUT2 and B3GLCT	1379:1451	The methods described include creation of CRISPR/Cas9-mediated knockout cell lines of POFUT2 and B3GLCT and use of these cell lines to analyze and quantify secretion defects of ADAMTS proteins.
31463900	8	59	theme	lines	1426:1430	arg1	use					1457:1459	use	1457:1459	use of these cell lines to analyze and quantify secretion defects of ADAMTS proteins	1457:1540	The methods described include creation of CRISPR/Cas9-mediated knockout cell lines of POFUT2 and B3GLCT and use of these cell lines to analyze and quantify secretion defects of ADAMTS proteins.
31463900	8	60	theme	CRISPR/Cas9-mediated	1391:1410	arg1	lines					1426:1430	CRISPR/Cas9-mediated knockout cell lines	1391:1430	CRISPR/Cas9-mediated knockout cell lines	1391:1430	The methods described include creation of CRISPR/Cas9-mediated knockout cell lines of POFUT2 and B3GLCT and use of these cell lines to analyze and quantify secretion defects of ADAMTS proteins.
31463900	4	61	from	glycosylation	901:913	arg1	humans					918:923	humans	918:923	humans	918:923	Knockout of POFUT2 results in embryonic lethality in mice, and inactivating mutations in B3GLCT cause Peters plus syndrome, a congenital disorder of glycosylation in humans.
31463900	6	62	theme	ADAMTS	1186:1191	arg1	proteins					1193:1200	ADAMTS proteins	1186:1200	ADAMTS proteins	1186:1200	Thus, POFUT2 and B3GLCT both function as an ER quality control pathway for folding of TSRs in ADAMTS proteins.
31463900	6	63	from	proteins	1193:1200	arg1	folding					1167:1173	folding	1167:1173	folding of TSRs in ADAMTS proteins	1167:1200	Thus, POFUT2 and B3GLCT both function as an ER quality control pathway for folding of TSRs in ADAMTS proteins.
31463900	8	64	theme	POFUT2	1435:1440	arg1	creation					1379:1386	creation	1379:1386	creation of CRISPR/Cas9-mediated knockout cell lines of POFUT2 and B3GLCT	1379:1451	The methods described include creation of CRISPR/Cas9-mediated knockout cell lines of POFUT2 and B3GLCT and use of these cell lines to analyze and quantify secretion defects of ADAMTS proteins.
31463900	8	64	theme	POFUT2	1435:1440	arg1	use					1457:1459	use	1457:1459	use of these cell lines to analyze and quantify secretion defects of ADAMTS proteins	1457:1540	The methods described include creation of CRISPR/Cas9-mediated knockout cell lines of POFUT2 and B3GLCT and use of these cell lines to analyze and quantify secretion defects of ADAMTS proteins.
31463900	4	65	theme	glycosylation	901:913	arg1	Peters					854:859	Peters plus syndrome	854:873	Peters	854:859	Knockout of POFUT2 results in embryonic lethality in mice, and inactivating mutations in B3GLCT cause Peters plus syndrome, a congenital disorder of glycosylation in humans.
31463900	4	65	theme	glycosylation	901:913	arg1	disorder					889:896	a congenital disorder	876:896	a congenital disorder of glycosylation in humans	876:923	Knockout of POFUT2 results in embryonic lethality in mice, and inactivating mutations in B3GLCT cause Peters plus syndrome, a congenital disorder of glycosylation in humans.
31463900	2	66	link	N-linked	427:434	arg1	glycosylation					436:448	N-linked glycosylation	427:448	N-linked glycosylation	427:448	Glycosylation occurs as these enzymes are trafficked through the endoplasmic reticulum (ER) and Golgi apparatus on their way to the extracellular space and includes N-linked glycosylation, O-linked fucosylation and C-linked mannosylation.
31463900	7	67	theme	POFUT2	1331:1336	arg1	loss					1316:1319	loss	1316:1319	loss of either POFUT2 or B3GLCT	1316:1346	In this chapter we describe in detail the methods developed to analyze secretion defects of ADAMTS proteins upon loss of either POFUT2 or B3GLCT.
31463900	8	68	theme	B3GLCT	1446:1451	arg1	creation					1379:1386	creation	1379:1386	creation of CRISPR/Cas9-mediated knockout cell lines of POFUT2 and B3GLCT	1379:1451	The methods described include creation of CRISPR/Cas9-mediated knockout cell lines of POFUT2 and B3GLCT and use of these cell lines to analyze and quantify secretion defects of ADAMTS proteins.
31463900	8	68	theme	B3GLCT	1446:1451	arg1	use					1457:1459	use	1457:1459	use of these cell lines to analyze and quantify secretion defects of ADAMTS proteins	1457:1540	The methods described include creation of CRISPR/Cas9-mediated knockout cell lines of POFUT2 and B3GLCT and use of these cell lines to analyze and quantify secretion defects of ADAMTS proteins.
31463900	0	69	theme	Proteins	63:70	arg1	Secretion					43:51	Secretion	43:51	Secretion of ADAMTS Proteins	43:70	Analyzing the Effects of O-Fucosylation on Secretion of ADAMTS Proteins Using Cell-Based Assays.
31463900	4	70	from	disorder	889:896	arg1	humans					918:923	humans	918:923	humans	918:923	Knockout of POFUT2 results in embryonic lethality in mice, and inactivating mutations in B3GLCT cause Peters plus syndrome, a congenital disorder of glycosylation in humans.
31463900	6	71	theme	TSRs	1178:1181	arg1	folding					1167:1173	folding	1167:1173	folding of TSRs in ADAMTS proteins	1167:1200	Thus, POFUT2 and B3GLCT both function as an ER quality control pathway for folding of TSRs in ADAMTS proteins.
31463900	7	72	theme	secretion	1274:1282	arg1	defects					1284:1290	secretion defects	1274:1290	secretion defects of ADAMTS proteins	1274:1309	In this chapter we describe in detail the methods developed to analyze secretion defects of ADAMTS proteins upon loss of either POFUT2 or B3GLCT.
31463900	1	73	theme	glycan	245:250	arg1	moieties					252:259	glycan moieties	245:259	glycan moieties	245:259	Metalloproteinases of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin type 1 repeats) superfamily are extensively modified with glycan moieties.
31463900	0	74	theme	ADAMTS	56:61	arg1	Proteins					63:70	ADAMTS Proteins	56:70	ADAMTS Proteins	56:70	Analyzing the Effects of O-Fucosylation on Secretion of ADAMTS Proteins Using Cell-Based Assays.
31463900	3	75	theme	Glucoseβ1-3Fucose	685:701	arg1	disaccharide					703:714	a Glucoseβ1-3Fucose disaccharide	683:714	a Glucoseβ1-3Fucose disaccharide	683:714	This chapter focuses on O-linked fucose, which is added to properly folded thrombospondin type 1 repeats (TSRs) in the ER by protein O-fucosyltransferase 2 (POFUT2) and elongated to a Glucoseβ1-3Fucose disaccharide by β3-glucosyltransferase (B3GLCT).
31463900	5	76	theme	folding	1010:1016	arg1	efficiency					1042:1051	folding in the ER and secretion efficiency	1010:1051	folding in the ER and secretion efficiency of several ADAMTS proteins from cells	1010:1089	Addition of the disaccharide by POFUT2 and B3GLCT stabilizes folded TSRs, enhancing folding in the ER and secretion efficiency of several ADAMTS proteins from cells.
31463900	6	77	from	TSRs	1178:1181	arg1	proteins					1193:1200	ADAMTS proteins	1186:1200	ADAMTS proteins	1186:1200	Thus, POFUT2 and B3GLCT both function as an ER quality control pathway for folding of TSRs in ADAMTS proteins.
32608473	8	0	theme	Saccharomyces	1071:1083	arg1	diversity					1010:1018	a lower alpha (α) diversity	992:1018	a lower alpha (α) diversity in mycobiota composition	992:1043	RESULTS Patients with pouchitis had a lower alpha (α) diversity in mycobiota composition and a higher abundance of Saccharomyces at the genus level compared with those with a normal pouch.
32608473	8	0	theme	Saccharomyces	1071:1083	arg1	abundance					1058:1066	a higher abundance	1049:1066	a higher abundance of Saccharomyces	1049:1083	RESULTS Patients with pouchitis had a lower alpha (α) diversity in mycobiota composition and a higher abundance of Saccharomyces at the genus level compared with those with a normal pouch.
32608473	4	1	theme	5	542:542	arg1	%					543:543	%	543:543	%	543:543	Experimental pouchitis was induced by 5% dextran sulfate sodium for 7 consecutive days in a rat model of IPAA.
32608473	4	2	theme	dextran	545:551	arg1	sodium					561:566	5% dextran sulfate sodium	542:566	5% dextran sulfate sodium for 7 consecutive days	542:589	Experimental pouchitis was induced by 5% dextran sulfate sodium for 7 consecutive days in a rat model of IPAA.
32608473	9	3	theme	increased	1336:1344	arg1	Polythrincium					1346:1358	Polythrincium	1346:1358	Polythrincium	1346:1358	In the rat model of pouchitis, Flu treatment decreased fungal burden but induced fungal dysbiosis, characterized by increased α diversity, a decreased relative abundance of Kazachstania, and increased Polythrincium and Saccharomyces.
32608473	6	4	theme	spacer	815:820	arg1	sequencing					824:833	internal transcribed spacer 2 sequencing	794:833	internal transcribed spacer 2 sequencing	794:833	Fecal fungal composition was analyzed using internal transcribed spacer 2 sequencing.
32608473	12	5	theme	necrosis	1677:1684	arg1	factor-α					1686:1693	tumor necrosis factor-α	1671:1693	tumor necrosis factor-α	1671:1693	In the Flu and laminarin groups, the expression of interferon-γ, tumor necrosis factor-α, CARD9, and phosphorylated nuclear factor kappa-B inhibitor alpha was decreased.
32608473	1	6	theme	pouch	171:175	arg1	IPAA					195:198	IPAA	195:198	IPAA	195:198	BACKGROUND Although the interaction between gut microbiota and pouchitis after ileal pouch anal anastomosis (IPAA) for ulcerative colitis (UC) has been confirmed, evidence of commensal mycobiota in the etiology of pouchitis is still lacking.
32608473	1	6	theme	pouch	171:175	arg1	anastomosis					182:192	ileal pouch anal anastomosis	165:192	ileal pouch anal anastomosis (IPAA) for ulcerative colitis (UC)	165:227	BACKGROUND Although the interaction between gut microbiota and pouchitis after ileal pouch anal anastomosis (IPAA) for ulcerative colitis (UC) has been confirmed, evidence of commensal mycobiota in the etiology of pouchitis is still lacking.
32608473	6	7	theme	internal	794:801	arg1	spacer					815:820	internal transcribed spacer	794:820	internal transcribed spacer 2 sequencing	794:833	Fecal fungal composition was analyzed using internal transcribed spacer 2 sequencing.
32608473	8	8	with	Patients	964:971	arg1	pouchitis					978:986	pouchitis	978:986	pouchitis	978:986	RESULTS Patients with pouchitis had a lower alpha (α) diversity in mycobiota composition and a higher abundance of Saccharomyces at the genus level compared with those with a normal pouch.
32608473	4	9	theme	IPAA	609:612	arg1	model					600:604	a rat model	594:604	a rat model of IPAA	594:612	Experimental pouchitis was induced by 5% dextran sulfate sodium for 7 consecutive days in a rat model of IPAA.
32608473	1	10	from	evidence	249:256	arg1	etiology					288:295	the etiology	284:295	the etiology of pouchitis	284:308	BACKGROUND Although the interaction between gut microbiota and pouchitis after ileal pouch anal anastomosis (IPAA) for ulcerative colitis (UC) has been confirmed, evidence of commensal mycobiota in the etiology of pouchitis is still lacking.
32608473	10	11	theme	higher	1499:1504	arg1	score					1519:1523	higher histological score	1499:1523	higher histological score	1499:1523	In addition, Flu treatment worsened dextran sulfate sodium pouchitis, as indicated by increased mortality, weight loss, higher histological score, and CD4+ cell infiltration.
32608473	8	12	theme	normal	1131:1136	arg1	pouch					1138:1142	a normal pouch	1129:1142	a normal pouch	1129:1142	RESULTS Patients with pouchitis had a lower alpha (α) diversity in mycobiota composition and a higher abundance of Saccharomyces at the genus level compared with those with a normal pouch.
32608473	10	13	theme	cell	1535:1538	arg1	infiltration					1540:1551	CD4+ cell infiltration	1530:1551	CD4+ cell infiltration	1530:1551	In addition, Flu treatment worsened dextran sulfate sodium pouchitis, as indicated by increased mortality, weight loss, higher histological score, and CD4+ cell infiltration.
32608473	5	14	theme	%	650:650	arg1	Flu					665:667	Flu	665:667	Flu	665:667	Fungal dysbiosis was induced by 0.5% fluconazole (Flu), and commensal fungal recognition through dectin-1 was blocked by 5% laminarin.
32608473	5	14	theme	%	650:650	arg1	fluconazole					652:662	0.5% fluconazole	647:662	0.5% fluconazole (Flu)	647:668	Fungal dysbiosis was induced by 0.5% fluconazole (Flu), and commensal fungal recognition through dectin-1 was blocked by 5% laminarin.
32608473	9	15	theme	rat	1152:1154	arg1	model					1156:1160	the rat model	1148:1160	the rat model of pouchitis	1148:1173	In the rat model of pouchitis, Flu treatment decreased fungal burden but induced fungal dysbiosis, characterized by increased α diversity, a decreased relative abundance of Kazachstania, and increased Polythrincium and Saccharomyces.
32608473	5	16	theme	Fungal	615:620	arg1	dysbiosis					622:630	Fungal dysbiosis	615:630	Fungal dysbiosis	615:630	Fungal dysbiosis was induced by 0.5% fluconazole (Flu), and commensal fungal recognition through dectin-1 was blocked by 5% laminarin.
32608473	7	17	theme	different	938:946	arg1	groups					948:953	different groups	938:953	different groups	938:953	Severity of pouchitis and activation of the CARD9-nuclear factor kappa-B pathway was determined among different groups.
32608473	12	18	theme	laminarin	1621:1629	arg1	groups					1631:1636	the Flu and laminarin groups	1609:1636	groups	1631:1636	In the Flu and laminarin groups, the expression of interferon-γ, tumor necrosis factor-α, CARD9, and phosphorylated nuclear factor kappa-B inhibitor alpha was decreased.
32608473	1	19	theme	gut	130:132	arg1	microbiota					134:143	gut microbiota	130:143	gut microbiota	130:143	BACKGROUND Although the interaction between gut microbiota and pouchitis after ileal pouch anal anastomosis (IPAA) for ulcerative colitis (UC) has been confirmed, evidence of commensal mycobiota in the etiology of pouchitis is still lacking.
32608473	8	20	from	diversity	1010:1018	arg1	composition					1033:1043	mycobiota composition	1023:1043	mycobiota composition	1023:1043	RESULTS Patients with pouchitis had a lower alpha (α) diversity in mycobiota composition and a higher abundance of Saccharomyces at the genus level compared with those with a normal pouch.
32608473	7	21	theme	pathway	909:915	arg1	activation					862:871	activation	862:871	activation	862:871	Severity of pouchitis and activation of the CARD9-nuclear factor kappa-B pathway was determined among different groups.
32608473	7	21	theme	pathway	909:915	arg1	pouchitis					848:856	pouchitis	848:856	pouchitis	848:856	Severity of pouchitis and activation of the CARD9-nuclear factor kappa-B pathway was determined among different groups.
32608473	0	22	theme	Pouch	62:66	arg1	Anastomosis					73:83	Ileal Pouch Anal Anastomosis	56:83	Ileal Pouch Anal Anastomosis	56:83	Fungal Dysbiosis Aggravates Pouchitis in a Rat Model of Ileal Pouch Anal Anastomosis.
32608473	9	23	theme	fungal	1200:1205	arg1	burden					1207:1212	fungal burden	1200:1212	fungal burden	1200:1212	In the rat model of pouchitis, Flu treatment decreased fungal burden but induced fungal dysbiosis, characterized by increased α diversity, a decreased relative abundance of Kazachstania, and increased Polythrincium and Saccharomyces.
32608473	7	24	theme	factor	894:899	arg1	pathway					909:915	the CARD9-nuclear factor kappa-B pathway	876:915	the CARD9-nuclear factor kappa-B pathway	876:915	Severity of pouchitis and activation of the CARD9-nuclear factor kappa-B pathway was determined among different groups.
32608473	12	25	theme	Flu	1613:1615	arg1	groups					1631:1636	the Flu and laminarin groups	1609:1636	groups	1631:1636	In the Flu and laminarin groups, the expression of interferon-γ, tumor necrosis factor-α, CARD9, and phosphorylated nuclear factor kappa-B inhibitor alpha was decreased.
32608473	13	26	theme	fungal	1824:1829	arg1	composition					1831:1841	altered fungal composition	1816:1841	altered fungal composition	1816:1841	CONCLUSIONS Patients with pouchitis had altered fungal composition.
32608473	0	27	theme	Anastomosis	73:83	arg1	Model					47:51	a Rat Model	41:51	a Rat Model of Ileal Pouch Anal Anastomosis	41:83	Fungal Dysbiosis Aggravates Pouchitis in a Rat Model of Ileal Pouch Anal Anastomosis.
32608473	10	28	theme	Flu	1392:1394	arg1	treatment					1396:1404	Flu treatment	1392:1404	Flu treatment	1392:1404	In addition, Flu treatment worsened dextran sulfate sodium pouchitis, as indicated by increased mortality, weight loss, higher histological score, and CD4+ cell infiltration.
32608473	8	29	contain	had	988:990	arg1	Patients					964:971	Patients	964:971	Patients with pouchitis	964:986	RESULTS Patients with pouchitis had a lower alpha (α) diversity in mycobiota composition and a higher abundance of Saccharomyces at the genus level compared with those with a normal pouch.
32608473	8	29	contain	had	988:990	arg2	abundance					1058:1066	a higher abundance	1049:1066	a higher abundance of Saccharomyces	1049:1083	RESULTS Patients with pouchitis had a lower alpha (α) diversity in mycobiota composition and a higher abundance of Saccharomyces at the genus level compared with those with a normal pouch.
32608473	8	29	contain	had	988:990	arg2	diversity					1010:1018	a lower alpha (α) diversity	992:1018	a lower alpha (α) diversity in mycobiota composition	992:1043	RESULTS Patients with pouchitis had a lower alpha (α) diversity in mycobiota composition and a higher abundance of Saccharomyces at the genus level compared with those with a normal pouch.
32608473	13	30	theme	CONCLUSIONS	1776:1786	arg1	Patients					1788:1795	CONCLUSIONS Patients	1776:1795	CONCLUSIONS Patients with pouchitis	1776:1810	CONCLUSIONS Patients with pouchitis had altered fungal composition.
32608473	9	31	theme	fungal	1226:1231	arg1	dysbiosis					1233:1241	fungal dysbiosis	1226:1241	fungal dysbiosis	1226:1241	In the rat model of pouchitis, Flu treatment decreased fungal burden but induced fungal dysbiosis, characterized by increased α diversity, a decreased relative abundance of Kazachstania, and increased Polythrincium and Saccharomyces.
32608473	7	32	theme	activation	862:871	arg1	Severity					836:843	Severity	836:843	Severity of pouchitis and activation of the CARD9-nuclear factor kappa-B pathway	836:915	Severity of pouchitis and activation of the CARD9-nuclear factor kappa-B pathway was determined among different groups.
32608473	2	33	theme	pouchitis	401:409	arg1	pathogenesis					385:396	the pathogenesis	381:396	the pathogenesis of pouchitis	381:409	This study aimed to investigate the role of fungi in the pathogenesis of pouchitis.
32608473	9	34	theme	increased	1261:1269	arg1	diversity					1273:1281	increased α diversity	1261:1281	increased α diversity	1261:1281	In the rat model of pouchitis, Flu treatment decreased fungal burden but induced fungal dysbiosis, characterized by increased α diversity, a decreased relative abundance of Kazachstania, and increased Polythrincium and Saccharomyces.
32608473	0	35	theme	Fungal	0:5	arg1	Dysbiosis					7:15	Fungal Dysbiosis	0:15	Fungal Dysbiosis	0:15	Fungal Dysbiosis Aggravates Pouchitis in a Rat Model of Ileal Pouch Anal Anastomosis.
32608473	8	36	theme	alpha	1000:1004	arg1	diversity					1010:1018	a lower alpha (α) diversity	992:1018	a lower alpha (α) diversity in mycobiota composition	992:1043	RESULTS Patients with pouchitis had a lower alpha (α) diversity in mycobiota composition and a higher abundance of Saccharomyces at the genus level compared with those with a normal pouch.
32608473	7	37	theme	pouchitis	848:856	arg1	Severity					836:843	Severity	836:843	Severity of pouchitis and activation of the CARD9-nuclear factor kappa-B pathway	836:915	Severity of pouchitis and activation of the CARD9-nuclear factor kappa-B pathway was determined among different groups.
32608473	4	38	theme	Experimental	504:515	arg1	pouchitis					517:525	Experimental pouchitis	504:525	Experimental pouchitis	504:525	Experimental pouchitis was induced by 5% dextran sulfate sodium for 7 consecutive days in a rat model of IPAA.
32608473	10	39	theme	sulfate	1423:1429	arg1	pouchitis					1438:1446	dextran sulfate sodium pouchitis	1415:1446	dextran sulfate sodium pouchitis	1415:1446	In addition, Flu treatment worsened dextran sulfate sodium pouchitis, as indicated by increased mortality, weight loss, higher histological score, and CD4+ cell infiltration.
32608473	3	40	theme	Fecal	420:424	arg1	samples					426:432	Fecal samples	420:432	Fecal samples	420:432	METHODS Fecal samples were collected from UC patients with or without pouchitis after IPAA.
32608473	5	41	theme	%	737:737	arg1	laminarin					739:747	5% laminarin	736:747	5% laminarin	736:747	Fungal dysbiosis was induced by 0.5% fluconazole (Flu), and commensal fungal recognition through dectin-1 was blocked by 5% laminarin.
32608473	8	42	theme	α	1007:1007	arg1	diversity					1010:1018	a lower alpha (α) diversity	992:1018	a lower alpha (α) diversity in mycobiota composition	992:1043	RESULTS Patients with pouchitis had a lower alpha (α) diversity in mycobiota composition and a higher abundance of Saccharomyces at the genus level compared with those with a normal pouch.
32608473	14	43	theme	experimental	1914:1925	arg1	pouchitis					1927:1935	experimental pouchitis	1914:1935	experimental pouchitis	1914:1935	Fungal dysbiosis or recognition deficiency by the host may exacerbate experimental pouchitis.
32608473	14	44	theme	dysbiosis	1851:1859	arg1	deficiency					1876:1885	Fungal dysbiosis or recognition deficiency	1844:1885	Fungal dysbiosis or recognition deficiency by the host	1844:1897	Fungal dysbiosis or recognition deficiency by the host may exacerbate experimental pouchitis.
32608473	1	45	theme	mycobiota	271:279	arg1	evidence					249:256	evidence	249:256	evidence of commensal mycobiota in the etiology of pouchitis	249:308	BACKGROUND Although the interaction between gut microbiota and pouchitis after ileal pouch anal anastomosis (IPAA) for ulcerative colitis (UC) has been confirmed, evidence of commensal mycobiota in the etiology of pouchitis is still lacking.
32608473	8	46	from	abundance	1058:1066	arg1	composition					1033:1043	mycobiota composition	1023:1043	mycobiota composition	1023:1043	RESULTS Patients with pouchitis had a lower alpha (α) diversity in mycobiota composition and a higher abundance of Saccharomyces at the genus level compared with those with a normal pouch.
32608473	12	47	theme	nuclear	1722:1728	arg1	alpha					1755:1759	phosphorylated nuclear factor kappa-B inhibitor alpha	1707:1759	phosphorylated nuclear factor kappa-B inhibitor alpha	1707:1759	In the Flu and laminarin groups, the expression of interferon-γ, tumor necrosis factor-α, CARD9, and phosphorylated nuclear factor kappa-B inhibitor alpha was decreased.
32608473	9	48	theme	α	1271:1271	arg1	diversity					1273:1281	increased α diversity	1261:1281	increased α diversity	1261:1281	In the rat model of pouchitis, Flu treatment decreased fungal burden but induced fungal dysbiosis, characterized by increased α diversity, a decreased relative abundance of Kazachstania, and increased Polythrincium and Saccharomyces.
32608473	0	49	theme	Rat	43:45	arg1	Model					47:51	a Rat Model	41:51	a Rat Model of Ileal Pouch Anal Anastomosis	41:83	Fungal Dysbiosis Aggravates Pouchitis in a Rat Model of Ileal Pouch Anal Anastomosis.
32608473	8	50	theme	mycobiota	1023:1031	arg1	composition					1033:1043	mycobiota composition	1023:1043	mycobiota composition	1023:1043	RESULTS Patients with pouchitis had a lower alpha (α) diversity in mycobiota composition and a higher abundance of Saccharomyces at the genus level compared with those with a normal pouch.
32608473	6	51	theme	fungal	756:761	arg1	composition					763:773	Fecal fungal composition	750:773	Fecal fungal composition	750:773	Fecal fungal composition was analyzed using internal transcribed spacer 2 sequencing.
32608473	12	52	theme	kappa-B	1737:1743	arg1	alpha					1755:1759	phosphorylated nuclear factor kappa-B inhibitor alpha	1707:1759	phosphorylated nuclear factor kappa-B inhibitor alpha	1707:1759	In the Flu and laminarin groups, the expression of interferon-γ, tumor necrosis factor-α, CARD9, and phosphorylated nuclear factor kappa-B inhibitor alpha was decreased.
32608473	1	53	theme	ulcerative	205:214	arg1	UC					225:226	UC	225:226	UC	225:226	BACKGROUND Although the interaction between gut microbiota and pouchitis after ileal pouch anal anastomosis (IPAA) for ulcerative colitis (UC) has been confirmed, evidence of commensal mycobiota in the etiology of pouchitis is still lacking.
32608473	1	53	theme	ulcerative	205:214	arg1	colitis					216:222	ulcerative colitis	205:222	ulcerative colitis (UC)	205:227	BACKGROUND Although the interaction between gut microbiota and pouchitis after ileal pouch anal anastomosis (IPAA) for ulcerative colitis (UC) has been confirmed, evidence of commensal mycobiota in the etiology of pouchitis is still lacking.
32608473	3	54	dep	METHODS	412:418	arg1	collected					439:447	collected	439:447	were collected from UC patients with or without pouchitis after IPAA	434:501	METHODS Fecal samples were collected from UC patients with or without pouchitis after IPAA.
32608473	12	55	theme	CARD9	1696:1700	arg1	expression					1643:1652	the expression	1639:1652	the expression of interferon-γ, tumor necrosis factor-α, CARD9, and phosphorylated nuclear factor kappa-B inhibitor alpha	1639:1759	In the Flu and laminarin groups, the expression of interferon-γ, tumor necrosis factor-α, CARD9, and phosphorylated nuclear factor kappa-B inhibitor alpha was decreased.
32608473	4	56	theme	sulfate	553:559	arg1	sodium					561:566	5% dextran sulfate sodium	542:566	5% dextran sulfate sodium for 7 consecutive days	542:589	Experimental pouchitis was induced by 5% dextran sulfate sodium for 7 consecutive days in a rat model of IPAA.
32608473	15	57	theme	therapeutic	1991:2001	arg1	potential					2003:2011	therapeutic potential	1991:2011	therapeutic potential	1991:2011	Strategies targeting commensal mycobiota may provide therapeutic potential against pouchitis, especially for antibiotic-refractory patients.
32608473	14	58	theme	Fungal	1844:1849	arg1	deficiency					1876:1885	Fungal dysbiosis or recognition deficiency	1844:1885	Fungal dysbiosis or recognition deficiency by the host	1844:1897	Fungal dysbiosis or recognition deficiency by the host may exacerbate experimental pouchitis.
32608473	8	59	theme	higher	1051:1056	arg1	abundance					1058:1066	a higher abundance	1049:1066	a higher abundance of Saccharomyces	1049:1083	RESULTS Patients with pouchitis had a lower alpha (α) diversity in mycobiota composition and a higher abundance of Saccharomyces at the genus level compared with those with a normal pouch.
32608473	5	60	theme	commensal	675:683	arg1	recognition					692:702	commensal fungal recognition	675:702	commensal fungal recognition through dectin-1	675:719	Fungal dysbiosis was induced by 0.5% fluconazole (Flu), and commensal fungal recognition through dectin-1 was blocked by 5% laminarin.
32608473	9	61	theme	Kazachstania	1318:1329	arg1	abundance					1305:1313	a decreased relative abundance	1284:1313	a decreased relative abundance of Kazachstania	1284:1329	In the rat model of pouchitis, Flu treatment decreased fungal burden but induced fungal dysbiosis, characterized by increased α diversity, a decreased relative abundance of Kazachstania, and increased Polythrincium and Saccharomyces.
32608473	9	61	theme	Kazachstania	1318:1329	arg1	diversity					1273:1281	increased α diversity	1261:1281	increased α diversity	1261:1281	In the rat model of pouchitis, Flu treatment decreased fungal burden but induced fungal dysbiosis, characterized by increased α diversity, a decreased relative abundance of Kazachstania, and increased Polythrincium and Saccharomyces.
32608473	9	61	theme	Kazachstania	1318:1329	arg1	Polythrincium					1346:1358	Polythrincium	1346:1358	Polythrincium	1346:1358	In the rat model of pouchitis, Flu treatment decreased fungal burden but induced fungal dysbiosis, characterized by increased α diversity, a decreased relative abundance of Kazachstania, and increased Polythrincium and Saccharomyces.
32608473	9	61	theme	Kazachstania	1318:1329	arg1	Saccharomyces					1364:1376	Saccharomyces	1364:1376	Saccharomyces	1364:1376	In the rat model of pouchitis, Flu treatment decreased fungal burden but induced fungal dysbiosis, characterized by increased α diversity, a decreased relative abundance of Kazachstania, and increased Polythrincium and Saccharomyces.
32608473	12	62	theme	tumor	1671:1675	arg1	factor-α					1686:1693	tumor necrosis factor-α	1671:1693	tumor necrosis factor-α	1671:1693	In the Flu and laminarin groups, the expression of interferon-γ, tumor necrosis factor-α, CARD9, and phosphorylated nuclear factor kappa-B inhibitor alpha was decreased.
32608473	12	63	theme	factor-α	1686:1693	arg1	expression					1643:1652	the expression	1639:1652	the expression of interferon-γ, tumor necrosis factor-α, CARD9, and phosphorylated nuclear factor kappa-B inhibitor alpha	1639:1759	In the Flu and laminarin groups, the expression of interferon-γ, tumor necrosis factor-α, CARD9, and phosphorylated nuclear factor kappa-B inhibitor alpha was decreased.
32608473	4	64	theme	%	543:543	arg1	sodium					561:566	5% dextran sulfate sodium	542:566	5% dextran sulfate sodium for 7 consecutive days	542:589	Experimental pouchitis was induced by 5% dextran sulfate sodium for 7 consecutive days in a rat model of IPAA.
32608473	10	65	theme	weight	1486:1491	arg1	loss					1493:1496	weight loss	1486:1496	weight loss	1486:1496	In addition, Flu treatment worsened dextran sulfate sodium pouchitis, as indicated by increased mortality, weight loss, higher histological score, and CD4+ cell infiltration.
32608473	11	66	theme	pouchitis	1595:1603	arg1	severity					1583:1590	the severity	1579:1590	the severity of pouchitis	1579:1603	Laminarin also increased the severity of pouchitis.
32608473	1	67	theme	ileal	165:169	arg1	IPAA					195:198	IPAA	195:198	IPAA	195:198	BACKGROUND Although the interaction between gut microbiota and pouchitis after ileal pouch anal anastomosis (IPAA) for ulcerative colitis (UC) has been confirmed, evidence of commensal mycobiota in the etiology of pouchitis is still lacking.
32608473	1	67	theme	ileal	165:169	arg1	anastomosis					182:192	ileal pouch anal anastomosis	165:192	ileal pouch anal anastomosis (IPAA) for ulcerative colitis (UC)	165:227	BACKGROUND Although the interaction between gut microbiota and pouchitis after ileal pouch anal anastomosis (IPAA) for ulcerative colitis (UC) has been confirmed, evidence of commensal mycobiota in the etiology of pouchitis is still lacking.
32608473	8	68	theme	genus	1092:1096	arg1	level					1098:1102	the genus level	1088:1102	the genus level	1088:1102	RESULTS Patients with pouchitis had a lower alpha (α) diversity in mycobiota composition and a higher abundance of Saccharomyces at the genus level compared with those with a normal pouch.
32608473	6	69	theme	transcribed	803:813	arg1	spacer					815:820	internal transcribed spacer	794:820	internal transcribed spacer 2 sequencing	794:833	Fecal fungal composition was analyzed using internal transcribed spacer 2 sequencing.
32608473	4	70	theme	rat	596:598	arg1	model					600:604	a rat model	594:604	a rat model of IPAA	594:612	Experimental pouchitis was induced by 5% dextran sulfate sodium for 7 consecutive days in a rat model of IPAA.
32608473	5	71	theme	fungal	685:690	arg1	recognition					692:702	commensal fungal recognition	675:702	commensal fungal recognition through dectin-1	675:719	Fungal dysbiosis was induced by 0.5% fluconazole (Flu), and commensal fungal recognition through dectin-1 was blocked by 5% laminarin.
32608473	12	72	theme	inhibitor	1745:1753	arg1	alpha					1755:1759	phosphorylated nuclear factor kappa-B inhibitor alpha	1707:1759	phosphorylated nuclear factor kappa-B inhibitor alpha	1707:1759	In the Flu and laminarin groups, the expression of interferon-γ, tumor necrosis factor-α, CARD9, and phosphorylated nuclear factor kappa-B inhibitor alpha was decreased.
32608473	1	73	theme	anal	177:180	arg1	IPAA					195:198	IPAA	195:198	IPAA	195:198	BACKGROUND Although the interaction between gut microbiota and pouchitis after ileal pouch anal anastomosis (IPAA) for ulcerative colitis (UC) has been confirmed, evidence of commensal mycobiota in the etiology of pouchitis is still lacking.
32608473	1	73	theme	anal	177:180	arg1	anastomosis					182:192	ileal pouch anal anastomosis	165:192	ileal pouch anal anastomosis (IPAA) for ulcerative colitis (UC)	165:227	BACKGROUND Although the interaction between gut microbiota and pouchitis after ileal pouch anal anastomosis (IPAA) for ulcerative colitis (UC) has been confirmed, evidence of commensal mycobiota in the etiology of pouchitis is still lacking.
32608473	12	74	theme	interferon-γ	1657:1668	arg1	expression					1643:1652	the expression	1639:1652	the expression of interferon-γ, tumor necrosis factor-α, CARD9, and phosphorylated nuclear factor kappa-B inhibitor alpha	1639:1759	In the Flu and laminarin groups, the expression of interferon-γ, tumor necrosis factor-α, CARD9, and phosphorylated nuclear factor kappa-B inhibitor alpha was decreased.
32608473	10	75	theme	histological	1506:1517	arg1	score					1519:1523	higher histological score	1499:1523	higher histological score	1499:1523	In addition, Flu treatment worsened dextran sulfate sodium pouchitis, as indicated by increased mortality, weight loss, higher histological score, and CD4+ cell infiltration.
32608473	4	76	theme	consecutive	574:584	arg1	days					586:589	7 consecutive days	572:589	7 consecutive days	572:589	Experimental pouchitis was induced by 5% dextran sulfate sodium for 7 consecutive days in a rat model of IPAA.
32608473	5	77	theme	0.5	647:649	arg1	%					650:650	%	650:650	%	650:650	Fungal dysbiosis was induced by 0.5% fluconazole (Flu), and commensal fungal recognition through dectin-1 was blocked by 5% laminarin.
32608473	10	78	theme	CD4+	1530:1533	arg1	infiltration					1540:1551	CD4+ cell infiltration	1530:1551	CD4+ cell infiltration	1530:1551	In addition, Flu treatment worsened dextran sulfate sodium pouchitis, as indicated by increased mortality, weight loss, higher histological score, and CD4+ cell infiltration.
32608473	13	79	with	Patients	1788:1795	arg1	pouchitis					1802:1810	pouchitis	1802:1810	pouchitis	1802:1810	CONCLUSIONS Patients with pouchitis had altered fungal composition.
32608473	9	80	theme	pouchitis	1165:1173	arg1	model					1156:1160	the rat model	1148:1160	the rat model of pouchitis	1148:1173	In the rat model of pouchitis, Flu treatment decreased fungal burden but induced fungal dysbiosis, characterized by increased α diversity, a decreased relative abundance of Kazachstania, and increased Polythrincium and Saccharomyces.
32608473	0	81	theme	Ileal	56:60	arg1	Anastomosis					73:83	Ileal Pouch Anal Anastomosis	56:83	Ileal Pouch Anal Anastomosis	56:83	Fungal Dysbiosis Aggravates Pouchitis in a Rat Model of Ileal Pouch Anal Anastomosis.
32608473	9	82	theme	Flu	1176:1178	arg1	treatment					1180:1188	Flu treatment	1176:1188	Flu treatment	1176:1188	In the rat model of pouchitis, Flu treatment decreased fungal burden but induced fungal dysbiosis, characterized by increased α diversity, a decreased relative abundance of Kazachstania, and increased Polythrincium and Saccharomyces.
32608473	9	83	theme	decreased	1286:1294	arg1	abundance					1305:1313	a decreased relative abundance	1284:1313	a decreased relative abundance of Kazachstania	1284:1329	In the rat model of pouchitis, Flu treatment decreased fungal burden but induced fungal dysbiosis, characterized by increased α diversity, a decreased relative abundance of Kazachstania, and increased Polythrincium and Saccharomyces.
32608473	0	84	theme	Anal	68:71	arg1	Anastomosis					73:83	Ileal Pouch Anal Anastomosis	56:83	Ileal Pouch Anal Anastomosis	56:83	Fungal Dysbiosis Aggravates Pouchitis in a Rat Model of Ileal Pouch Anal Anastomosis.
32608473	1	85	theme	pouchitis	300:308	arg1	etiology					288:295	the etiology	284:295	the etiology of pouchitis	284:308	BACKGROUND Although the interaction between gut microbiota and pouchitis after ileal pouch anal anastomosis (IPAA) for ulcerative colitis (UC) has been confirmed, evidence of commensal mycobiota in the etiology of pouchitis is still lacking.
32608473	13	86	theme	altered	1816:1822	arg1	composition					1831:1841	altered fungal composition	1816:1841	altered fungal composition	1816:1841	CONCLUSIONS Patients with pouchitis had altered fungal composition.
32608473	7	87	theme	kappa-B	901:907	arg1	pathway					909:915	the CARD9-nuclear factor kappa-B pathway	876:915	the CARD9-nuclear factor kappa-B pathway	876:915	Severity of pouchitis and activation of the CARD9-nuclear factor kappa-B pathway was determined among different groups.
32608473	8	88	dep	RESULTS	956:962	arg1	had					988:990	had	988:990	had a lower alpha (α) diversity in mycobiota composition and a higher abundance of Saccharomyces at the genus level	988:1102	RESULTS Patients with pouchitis had a lower alpha (α) diversity in mycobiota composition and a higher abundance of Saccharomyces at the genus level compared with those with a normal pouch.
32608473	8	89	theme	lower	994:998	arg1	diversity					1010:1018	a lower alpha (α) diversity	992:1018	a lower alpha (α) diversity in mycobiota composition	992:1043	RESULTS Patients with pouchitis had a lower alpha (α) diversity in mycobiota composition and a higher abundance of Saccharomyces at the genus level compared with those with a normal pouch.
32608473	7	90	theme	CARD9-nuclear	880:892	arg1	pathway					909:915	the CARD9-nuclear factor kappa-B pathway	876:915	the CARD9-nuclear factor kappa-B pathway	876:915	Severity of pouchitis and activation of the CARD9-nuclear factor kappa-B pathway was determined among different groups.
32608473	13	91	contain	had	1812:1814	arg1	Patients					1788:1795	CONCLUSIONS Patients	1776:1795	CONCLUSIONS Patients with pouchitis	1776:1810	CONCLUSIONS Patients with pouchitis had altered fungal composition.
32608473	13	91	contain	had	1812:1814	arg2	composition					1831:1841	altered fungal composition	1816:1841	altered fungal composition	1816:1841	CONCLUSIONS Patients with pouchitis had altered fungal composition.
32608473	5	92	theme	5	736:736	arg1	%					737:737	%	737:737	%	737:737	Fungal dysbiosis was induced by 0.5% fluconazole (Flu), and commensal fungal recognition through dectin-1 was blocked by 5% laminarin.
32608473	15	93	theme	antibiotic-refractory	2047:2067	arg1	patients					2069:2076	antibiotic-refractory patients	2047:2076	antibiotic-refractory patients	2047:2076	Strategies targeting commensal mycobiota may provide therapeutic potential against pouchitis, especially for antibiotic-refractory patients.
32608473	10	94	theme	dextran	1415:1421	arg1	sulfate					1423:1429	dextran sulfate	1415:1429	dextran sulfate sodium pouchitis	1415:1446	In addition, Flu treatment worsened dextran sulfate sodium pouchitis, as indicated by increased mortality, weight loss, higher histological score, and CD4+ cell infiltration.
32608473	2	95	theme	fungi	372:376	arg1	role					364:367	the role	360:367	the role of fungi in the pathogenesis of pouchitis	360:409	This study aimed to investigate the role of fungi in the pathogenesis of pouchitis.
32608473	1	96	theme	commensal	261:269	arg1	mycobiota					271:279	commensal mycobiota	261:279	commensal mycobiota	261:279	BACKGROUND Although the interaction between gut microbiota and pouchitis after ileal pouch anal anastomosis (IPAA) for ulcerative colitis (UC) has been confirmed, evidence of commensal mycobiota in the etiology of pouchitis is still lacking.
32608473	15	97	theme	commensal	1959:1967	arg1	mycobiota					1969:1977	commensal mycobiota	1959:1977	commensal mycobiota	1959:1977	Strategies targeting commensal mycobiota may provide therapeutic potential against pouchitis, especially for antibiotic-refractory patients.
32608473	12	98	theme	alpha	1755:1759	arg1	expression					1643:1652	the expression	1639:1652	the expression of interferon-γ, tumor necrosis factor-α, CARD9, and phosphorylated nuclear factor kappa-B inhibitor alpha	1639:1759	In the Flu and laminarin groups, the expression of interferon-γ, tumor necrosis factor-α, CARD9, and phosphorylated nuclear factor kappa-B inhibitor alpha was decreased.
32608473	12	99	theme	phosphorylated	1707:1720	arg1	alpha					1755:1759	phosphorylated nuclear factor kappa-B inhibitor alpha	1707:1759	phosphorylated nuclear factor kappa-B inhibitor alpha	1707:1759	In the Flu and laminarin groups, the expression of interferon-γ, tumor necrosis factor-α, CARD9, and phosphorylated nuclear factor kappa-B inhibitor alpha was decreased.
32608473	3	100	theme	UC	454:455	arg1	patients					457:464	UC patients	454:464	UC patients with or without pouchitis after IPAA	454:501	METHODS Fecal samples were collected from UC patients with or without pouchitis after IPAA.
32608473	6	101	theme	Fecal	750:754	arg1	composition					763:773	Fecal fungal composition	750:773	Fecal fungal composition	750:773	Fecal fungal composition was analyzed using internal transcribed spacer 2 sequencing.
32608473	12	102	theme	factor	1730:1735	arg1	alpha					1755:1759	phosphorylated nuclear factor kappa-B inhibitor alpha	1707:1759	phosphorylated nuclear factor kappa-B inhibitor alpha	1707:1759	In the Flu and laminarin groups, the expression of interferon-γ, tumor necrosis factor-α, CARD9, and phosphorylated nuclear factor kappa-B inhibitor alpha was decreased.
32608473	10	103	theme	sodium	1431:1436	arg1	pouchitis					1438:1446	dextran sulfate sodium pouchitis	1415:1446	dextran sulfate sodium pouchitis	1415:1446	In addition, Flu treatment worsened dextran sulfate sodium pouchitis, as indicated by increased mortality, weight loss, higher histological score, and CD4+ cell infiltration.
32608473	9	104	theme	relative	1296:1303	arg1	abundance					1305:1313	a decreased relative abundance	1284:1313	a decreased relative abundance of Kazachstania	1284:1329	In the rat model of pouchitis, Flu treatment decreased fungal burden but induced fungal dysbiosis, characterized by increased α diversity, a decreased relative abundance of Kazachstania, and increased Polythrincium and Saccharomyces.
32608473	14	105	theme	recognition	1864:1874	arg1	deficiency					1876:1885	Fungal dysbiosis or recognition deficiency	1844:1885	Fungal dysbiosis or recognition deficiency by the host	1844:1897	Fungal dysbiosis or recognition deficiency by the host may exacerbate experimental pouchitis.
32608473	2	106	from	role	364:367	arg1	pathogenesis					385:396	the pathogenesis	381:396	the pathogenesis of pouchitis	381:409	This study aimed to investigate the role of fungi in the pathogenesis of pouchitis.
32608473	10	107	theme	increased	1465:1473	arg1	mortality					1475:1483	increased mortality	1465:1483	increased mortality	1465:1483	In addition, Flu treatment worsened dextran sulfate sodium pouchitis, as indicated by increased mortality, weight loss, higher histological score, and CD4+ cell infiltration.
34817339	0	0	from	effects	10:16	arg1	Oxidase					32:38	Polyphenol Oxidase	21:38	Polyphenol Oxidase	21:38	Metal ion effects on Polyphenol Oxidase Covalently immobilized on a Bio-Composite.
34817339	12	1	theme	x10-3	1117:1121	arg1	mol/L					1123:1127	19.69x10-3 mol/L and 23.49 x10-3 mol/L	1090:1127	19.69x10-3 mol/L and 23.49 x10-3 mol/L	1090:1127	Co+3 and Cu+1 had very strong inhibitory effects with IC50 values of 19.69x10-3 mol/L and 23.49 x10-3 mol/L, respectively.
34817339	13	2	theme	metal	1194:1198	arg1	ions					1200:1203	metal ions	1194:1203	metal ions	1194:1203	Inhibition constants (Ki) and inhibition types of metal ions were determined with immobilized enzyme.
34817339	10	3	theme	x10-6	940:944	arg1	mol/L					946:950	mol/L	946:950	mol/L	946:950	Concentration range of metal ions is 1.0-10.0 x10-6 mol/L.
34817339	13	4	theme	immobilized	1226:1236	arg1	enzyme					1238:1243	immobilized enzyme	1226:1243	immobilized enzyme	1226:1243	Inhibition constants (Ki) and inhibition types of metal ions were determined with immobilized enzyme.
34817339	6	5	from	storage	592:598	arg1	enzyme					634:639	immobilized and free enzyme	613:639	immobilized and free enzyme	613:639	Various parameters were studied such as pH, temperature and storage stability on immobilized and free enzyme.
34817339	3	6	theme	Polyphenol	253:262	arg1	PPO					273:275	PPO	273:275	PPO	273:275	Polyphenol oxidase (PPO) was used generally in biosensor applications.
34817339	3	6	theme	Polyphenol	253:262	arg1	oxidase					264:270	Polyphenol oxidase	253:270	Polyphenol oxidase (PPO)	253:276	Polyphenol oxidase (PPO) was used generally in biosensor applications.
34817339	4	7	theme	chitosan-gelatin	416:431	arg1	bio-composite					433:445	chitosan-gelatin bio-composite	416:445	chitosan-gelatin bio-composite	416:445	For this purpose, Polyphenol oxidase from banana was purified and covalently immobilized on chitosan-gelatin bio-composite.
34817339	10	8	theme	1.0-10.0	931:938	arg1	mol/L					946:950	mol/L	946:950	mol/L	946:950	Concentration range of metal ions is 1.0-10.0 x10-6 mol/L.
34817339	7	9	theme	different	684:692	arg1	substrates					694:703	different substrates	684:703	different substrates on immobilized and free enzyme	684:734	Kinetic parameters were also evaluated by different substrates on immobilized and free enzyme.
34817339	6	10	dep	pH	572:573	arg1	stability					600:608	stability	600:608	stability	600:608	Various parameters were studied such as pH, temperature and storage stability on immobilized and free enzyme.
34817339	15	11	with	substrate	1449:1457	arg1	value					1483:1487	3.33x10-5 mol/L Ki value	1464:1487	3.33x10-5 mol/L Ki value on immobilized PPO	1464:1506	Mixed type inhibition was obtained with Co+3 ion using catechol as substrate with 3.33x10-5 mol/L Ki value on immobilized PPO.
34817339	5	12	theme	immobilized	466:476	arg1	enzyme					478:483	immobilized enzyme	466:483	immobilized enzyme	466:483	The properties of immobilized enzyme were investigated and compared to free enzyme.
34817339	11	13	theme	PPO	981:983	arg1	activity					957:964	The activity	953:964	The activity of immobilized PPO	953:983	The activity of immobilized PPO was increased by Fe+2 and Ag+1 ion.
34817339	16	14	theme	measurements	1575:1586	arg1	purpose					1564:1570	the purpose	1560:1570	the purpose of measurements of metal ions	1560:1600	Immobilized PPO can be evaluated for biosensor for the purpose of measurements of metal ions.
34817339	2	15	theme	immobilization	163:176	arg1	methods					178:184	immobilization methods	163:184	immobilization methods	163:184	Interest in immobilization methods have increased because biosensors have been important for science.
34817339	9	16	theme	immobilized	874:884	arg1	enzyme					886:891	immobilized enzyme	874:891	immobilized enzyme	874:891	In vitro effects of metal ions were studied on immobilized enzyme.
34817339	7	17	theme	Kinetic	642:648	arg1	parameters					650:659	Kinetic parameters	642:659	Kinetic parameters	642:659	Kinetic parameters were also evaluated by different substrates on immobilized and free enzyme.
34817339	9	18	dep	In	827:828	arg1	vitro					830:834	vitro	830:834	vitro	830:834	In vitro effects of metal ions were studied on immobilized enzyme.
34817339	14	19	theme	non-competitive	1330:1344	arg1	inhibition					1346:1355	non-competitive inhibition	1330:1355	non-competitive inhibition with immobilized enzyme	1330:1379	Zn+2 and Cr+3 ions were showed competitive inhibition and Pb+2 ions were determined non-competitive inhibition with immobilized enzyme.
34817339	6	20	from	pH	572:573	arg1	enzyme					634:639	immobilized and free enzyme	613:639	immobilized and free enzyme	613:639	Various parameters were studied such as pH, temperature and storage stability on immobilized and free enzyme.
34817339	14	21	theme	immobilized	1362:1372	arg1	enzyme					1374:1379	immobilized enzyme	1362:1379	immobilized enzyme	1362:1379	Zn+2 and Cr+3 ions were showed competitive inhibition and Pb+2 ions were determined non-competitive inhibition with immobilized enzyme.
34817339	16	22	theme	metal	1591:1595	arg1	ions					1597:1600	metal ions	1591:1600	metal ions	1591:1600	Immobilized PPO can be evaluated for biosensor for the purpose of measurements of metal ions.
34817339	11	23	theme	immobilized	969:979	arg1	PPO					981:983	immobilized PPO	969:983	immobilized PPO	969:983	The activity of immobilized PPO was increased by Fe+2 and Ag+1 ion.
34817339	15	24	theme	type	1388:1391	arg1	inhibition					1393:1402	Mixed type inhibition	1382:1402	Mixed type inhibition	1382:1402	Mixed type inhibition was obtained with Co+3 ion using catechol as substrate with 3.33x10-5 mol/L Ki value on immobilized PPO.
34817339	0	25	theme	ion	6:8	arg1	effects					10:16	Metal ion effects	0:16	Metal ion effects on Polyphenol Oxidase	0:38	Metal ion effects on Polyphenol Oxidase Covalently immobilized on a Bio-Composite.
34817339	15	26	theme	Ki	1480:1481	arg1	value					1483:1487	3.33x10-5 mol/L Ki value	1464:1487	3.33x10-5 mol/L Ki value on immobilized PPO	1464:1506	Mixed type inhibition was obtained with Co+3 ion using catechol as substrate with 3.33x10-5 mol/L Ki value on immobilized PPO.
34817339	14	27	theme	competitive	1277:1287	arg1	inhibition					1289:1298	competitive inhibition	1277:1298	competitive inhibition	1277:1298	Zn+2 and Cr+3 ions were showed competitive inhibition and Pb+2 ions were determined non-competitive inhibition with immobilized enzyme.
34817339	15	28	theme	Mixed	1382:1386	arg1	inhibition					1393:1402	Mixed type inhibition	1382:1402	Mixed type inhibition	1382:1402	Mixed type inhibition was obtained with Co+3 ion using catechol as substrate with 3.33x10-5 mol/L Ki value on immobilized PPO.
34817339	0	29	theme	Metal	0:4	arg1	effects					10:16	Metal ion effects	0:16	Metal ion effects on Polyphenol Oxidase	0:38	Metal ion effects on Polyphenol Oxidase Covalently immobilized on a Bio-Composite.
34817339	8	30	theme	best	765:768	arg1	substrate					770:778	the best substrate	761:778	the best substrate for immobilized enzyme with optimum condition	761:824	Catechol was determined the best substrate for immobilized enzyme with optimum condition.
34817339	13	31	theme	Inhibition	1144:1153	arg1	Ki					1166:1167	Ki	1166:1167	Ki	1166:1167	Inhibition constants (Ki) and inhibition types of metal ions were determined with immobilized enzyme.
34817339	13	31	theme	Inhibition	1144:1153	arg1	constants					1155:1163	Inhibition constants	1144:1163	Inhibition constants (Ki)	1144:1168	Inhibition constants (Ki) and inhibition types of metal ions were determined with immobilized enzyme.
34817339	14	32	with	inhibition	1346:1355	arg1	enzyme					1374:1379	immobilized enzyme	1362:1379	immobilized enzyme	1362:1379	Zn+2 and Cr+3 ions were showed competitive inhibition and Pb+2 ions were determined non-competitive inhibition with immobilized enzyme.
34817339	12	33	theme	mol/L	1123:1127	arg1	values					1080:1085	IC50 values	1075:1085	IC50 values	1075:1085	Co+3 and Cu+1 had very strong inhibitory effects with IC50 values of 19.69x10-3 mol/L and 23.49 x10-3 mol/L, respectively.
34817339	6	34	theme	free	629:632	arg1	enzyme					634:639	immobilized and free enzyme	613:639	immobilized and free enzyme	613:639	Various parameters were studied such as pH, temperature and storage stability on immobilized and free enzyme.
34817339	15	35	theme	mol/L	1474:1478	arg1	value					1483:1487	3.33x10-5 mol/L Ki value	1464:1487	3.33x10-5 mol/L Ki value on immobilized PPO	1464:1506	Mixed type inhibition was obtained with Co+3 ion using catechol as substrate with 3.33x10-5 mol/L Ki value on immobilized PPO.
34817339	12	36	contain	had	1035:1037	arg1	Co+3					1021:1024	Co+3	1021:1024	Co+3	1021:1024	Co+3 and Cu+1 had very strong inhibitory effects with IC50 values of 19.69x10-3 mol/L and 23.49 x10-3 mol/L, respectively.
34817339	12	36	contain	had	1035:1037	arg2	effects					1062:1068	very strong inhibitory effects	1039:1068	very strong inhibitory effects	1039:1068	Co+3 and Cu+1 had very strong inhibitory effects with IC50 values of 19.69x10-3 mol/L and 23.49 x10-3 mol/L, respectively.
34817339	12	36	contain	had	1035:1037	arg1	Cu+1					1030:1033	Cu+1	1030:1033	Cu+1	1030:1033	Co+3 and Cu+1 had very strong inhibitory effects with IC50 values of 19.69x10-3 mol/L and 23.49 x10-3 mol/L, respectively.
34817339	14	37	theme	Pb+2	1304:1307	arg1	ions					1309:1312	Pb+2 ions	1304:1312	Pb+2 ions	1304:1312	Zn+2 and Cr+3 ions were showed competitive inhibition and Pb+2 ions were determined non-competitive inhibition with immobilized enzyme.
34817339	5	38	theme	free	519:522	arg1	enzyme					524:529	free enzyme	519:529	free enzyme	519:529	The properties of immobilized enzyme were investigated and compared to free enzyme.
34817339	10	39	theme	Concentration	894:906	arg1	range					908:912	Concentration range	894:912	Concentration range of metal ions	894:926	Concentration range of metal ions is 1.0-10.0 x10-6 mol/L.
34817339	2	40	from	Interest	151:158	arg1	methods					178:184	immobilization methods	163:184	immobilization methods	163:184	Interest in immobilization methods have increased because biosensors have been important for science.
34817339	10	41	theme	ions	923:926	arg1	range					908:912	Concentration range	894:912	Concentration range of metal ions	894:926	Concentration range of metal ions is 1.0-10.0 x10-6 mol/L.
34817339	6	42	theme	Various	532:538	arg1	parameters					540:549	Various parameters	532:549	Various parameters	532:549	Various parameters were studied such as pH, temperature and storage stability on immobilized and free enzyme.
34817339	0	43	theme	Polyphenol	21:30	arg1	Oxidase					32:38	Polyphenol Oxidase	21:38	Polyphenol Oxidase	21:38	Metal ion effects on Polyphenol Oxidase Covalently immobilized on a Bio-Composite.
34817339	6	44	theme	immobilized	613:623	arg1	enzyme					634:639	immobilized and free enzyme	613:639	immobilized and free enzyme	613:639	Various parameters were studied such as pH, temperature and storage stability on immobilized and free enzyme.
34817339	4	45	from	banana	366:371	arg1	oxidase					353:359	Polyphenol oxidase	342:359	Polyphenol oxidase from banana	342:371	For this purpose, Polyphenol oxidase from banana was purified and covalently immobilized on chitosan-gelatin bio-composite.
34817339	13	46	theme	ions	1200:1203	arg1	Ki					1166:1167	Ki	1166:1167	Ki	1166:1167	Inhibition constants (Ki) and inhibition types of metal ions were determined with immobilized enzyme.
34817339	13	46	theme	ions	1200:1203	arg1	constants					1155:1163	Inhibition constants	1144:1163	Inhibition constants (Ki)	1144:1168	Inhibition constants (Ki) and inhibition types of metal ions were determined with immobilized enzyme.
34817339	13	46	theme	ions	1200:1203	arg1	types					1185:1189	inhibition types	1174:1189	inhibition types of metal ions	1174:1203	Inhibition constants (Ki) and inhibition types of metal ions were determined with immobilized enzyme.
34817339	7	47	from	substrates	694:703	arg1	enzyme					729:734	immobilized and free enzyme	708:734	immobilized and free enzyme	708:734	Kinetic parameters were also evaluated by different substrates on immobilized and free enzyme.
34817339	8	48	theme	immobilized	784:794	arg1	enzyme					796:801	immobilized enzyme	784:801	immobilized enzyme	784:801	Catechol was determined the best substrate for immobilized enzyme with optimum condition.
34817339	14	49	theme	Zn+2	1246:1249	arg1	ions					1260:1263	Zn+2 and Cr+3 ions	1246:1263	Zn+2 and Cr+3 ions	1246:1263	Zn+2 and Cr+3 ions were showed competitive inhibition and Pb+2 ions were determined non-competitive inhibition with immobilized enzyme.
34817339	9	50	theme	In	827:828	arg1	effects					836:842	In vitro effects	827:842	In vitro effects of metal ions	827:856	In vitro effects of metal ions were studied on immobilized enzyme.
34817339	12	51	theme	inhibitory	1051:1060	arg1	effects					1062:1068	very strong inhibitory effects	1039:1068	very strong inhibitory effects	1039:1068	Co+3 and Cu+1 had very strong inhibitory effects with IC50 values of 19.69x10-3 mol/L and 23.49 x10-3 mol/L, respectively.
34817339	14	52	theme	Cr+3	1255:1258	arg1	ions					1260:1263	Zn+2 and Cr+3 ions	1246:1263	Zn+2 and Cr+3 ions	1246:1263	Zn+2 and Cr+3 ions were showed competitive inhibition and Pb+2 ions were determined non-competitive inhibition with immobilized enzyme.
34817339	12	53	theme	IC50	1075:1078	arg1	values					1080:1085	IC50 values	1075:1085	IC50 values	1075:1085	Co+3 and Cu+1 had very strong inhibitory effects with IC50 values of 19.69x10-3 mol/L and 23.49 x10-3 mol/L, respectively.
34817339	8	54	theme	optimum	808:814	arg1	condition					816:824	optimum condition	808:824	optimum condition	808:824	Catechol was determined the best substrate for immobilized enzyme with optimum condition.
34817339	11	55	dep	Fe+2	1002:1005	arg1	ion					1016:1018	ion	1016:1018	ion	1016:1018	The activity of immobilized PPO was increased by Fe+2 and Ag+1 ion.
34817339	7	56	theme	free	724:727	arg1	enzyme					729:734	immobilized and free enzyme	708:734	immobilized and free enzyme	708:734	Kinetic parameters were also evaluated by different substrates on immobilized and free enzyme.
34817339	1	57	theme	different	117:125	arg1	methods					142:148	different immobilization methods	117:148	different immobilization methods	117:148	Biosensors can be developed using different immobilization methods.
34817339	5	58	theme	enzyme	478:483	arg1	properties					452:461	The properties	448:461	The properties of immobilized enzyme	448:483	The properties of immobilized enzyme were investigated and compared to free enzyme.
34817339	15	59	from	value	1483:1487	arg1	PPO					1504:1506	immobilized PPO	1492:1506	immobilized PPO	1492:1506	Mixed type inhibition was obtained with Co+3 ion using catechol as substrate with 3.33x10-5 mol/L Ki value on immobilized PPO.
34817339	3	60	theme	biosensor	300:308	arg1	applications					310:321	biosensor applications	300:321	biosensor applications	300:321	Polyphenol oxidase (PPO) was used generally in biosensor applications.
34817339	8	61	with	substrate	770:778	arg1	condition					816:824	optimum condition	808:824	optimum condition	808:824	Catechol was determined the best substrate for immobilized enzyme with optimum condition.
34817339	1	62	theme	immobilization	127:140	arg1	methods					142:148	different immobilization methods	117:148	different immobilization methods	117:148	Biosensors can be developed using different immobilization methods.
34817339	12	63	theme	strong	1044:1049	arg1	effects					1062:1068	very strong inhibitory effects	1039:1068	very strong inhibitory effects	1039:1068	Co+3 and Cu+1 had very strong inhibitory effects with IC50 values of 19.69x10-3 mol/L and 23.49 x10-3 mol/L, respectively.
34817339	7	64	theme	immobilized	708:718	arg1	enzyme					729:734	immobilized and free enzyme	708:734	immobilized and free enzyme	708:734	Kinetic parameters were also evaluated by different substrates on immobilized and free enzyme.
34817339	16	65	theme	ions	1597:1600	arg1	measurements					1575:1586	measurements	1575:1586	measurements of metal ions	1575:1600	Immobilized PPO can be evaluated for biosensor for the purpose of measurements of metal ions.
34817339	13	66	theme	inhibition	1174:1183	arg1	types					1185:1189	inhibition types	1174:1189	inhibition types of metal ions	1174:1203	Inhibition constants (Ki) and inhibition types of metal ions were determined with immobilized enzyme.
34817339	16	67	theme	Immobilized	1509:1519	arg1	PPO					1521:1523	Immobilized PPO	1509:1523	Immobilized PPO	1509:1523	Immobilized PPO can be evaluated for biosensor for the purpose of measurements of metal ions.
34817339	10	68	theme	metal	917:921	arg1	ions					923:926	metal ions	917:926	metal ions	917:926	Concentration range of metal ions is 1.0-10.0 x10-6 mol/L.
34817339	9	69	theme	metal	847:851	arg1	ions					853:856	metal ions	847:856	metal ions	847:856	In vitro effects of metal ions were studied on immobilized enzyme.
34817339	15	70	theme	immobilized	1492:1502	arg1	PPO					1504:1506	immobilized PPO	1492:1506	immobilized PPO	1492:1506	Mixed type inhibition was obtained with Co+3 ion using catechol as substrate with 3.33x10-5 mol/L Ki value on immobilized PPO.
34817339	6	71	from	temperature	576:586	arg1	enzyme					634:639	immobilized and free enzyme	613:639	immobilized and free enzyme	613:639	Various parameters were studied such as pH, temperature and storage stability on immobilized and free enzyme.
34817339	9	72	theme	ions	853:856	arg1	effects					836:842	In vitro effects	827:842	In vitro effects of metal ions	827:856	In vitro effects of metal ions were studied on immobilized enzyme.
34817339	4	73	theme	Polyphenol	342:351	arg1	oxidase					353:359	Polyphenol oxidase	342:359	Polyphenol oxidase from banana	342:371	For this purpose, Polyphenol oxidase from banana was purified and covalently immobilized on chitosan-gelatin bio-composite.
34817339	3	74	used	used	282:285	arg2	PPO					273:275	PPO	273:275	PPO	273:275	Polyphenol oxidase (PPO) was used generally in biosensor applications.
34817339	3	74	used	used	282:285	arg2	oxidase					264:270	Polyphenol oxidase	253:270	Polyphenol oxidase (PPO)	253:276	Polyphenol oxidase (PPO) was used generally in biosensor applications.
34817339	15	75	theme	Co+3	1422:1425	arg1	ion					1427:1429	Co+3 ion	1422:1429	Co+3 ion using catechol as substrate with 3.33x10-5 mol/L Ki value on immobilized PPO	1422:1506	Mixed type inhibition was obtained with Co+3 ion using catechol as substrate with 3.33x10-5 mol/L Ki value on immobilized PPO.
34817339	15	76	theme	3.33x10-5	1464:1472	arg1	mol/L					1474:1478	mol/L	1474:1478	mol/L	1474:1478	Mixed type inhibition was obtained with Co+3 ion using catechol as substrate with 3.33x10-5 mol/L Ki value on immobilized PPO.
33652358	6	0	theme	RAW	994:996	arg1	cells					1004:1008	lipopolysaccharide-stimulated RAW 264.7 cells	964:1008	lipopolysaccharide-stimulated RAW 264.7 cells	964:1008	Then, lipopolysaccharide-stimulated RAW 264.7 cells were used to screen effective anti-inflammatory ingredients.
33652358	13	1	dep	origins	2282:2288	arg1	The					2278:2280	The	2278:2280	The	2278:2280	The origins and consistency of D. odorifera could be efficiently discriminated by hierarchical cluster analysis (HCA).
33652358	7	2	from	Q-markers	1153:1161	arg1	samples					1179:1185	D. odorifera samples	1166:1185	D. odorifera samples	1166:1185	Finally, a UHPLC-HRMS method was developed and validated to quantify the selected Q-markers in D. odorifera samples.
33652358	10	3	theme	RAW	1682:1684	arg1	cells					1692:1696	RAW 264.7 cells	1682:1696	RAW 264.7 cells	1682:1696	It revealed that the extract of D. odorifera and nine flavonoids in the noncytotoxic range could alleviated lipopolysaccharide-stimulated inflammation in RAW 264.7 cells by decreasing the production of proinflammatory mediators such as nitric oxide and interleukin-6.
33652358	14	4	theme	comprehensive	2596:2608	arg1	assessment					2618:2627	a more comprehensive quality assessment	2589:2627	a more comprehensive quality assessment of D. odorifera	2589:2643	CONCLUSION The analysis strategy that combines phytochemical analysis with anti-inflammatory screening clarified the therapeutic material basis and discovered Q-markers, which possibly offers a more comprehensive quality assessment of D. odorifera.
33652358	9	5	theme	anti-inflammatory	1498:1514	arg1	activities					1516:1525	their anti-inflammatory activities	1492:1525	their anti-inflammatory activities	1492:1525	Among them, 31 compounds representing these 6 categories were further evaluated for their anti-inflammatory activities.
33652358	4	6	theme	"	679:679	arg1	strategy					692:699	a "phytochemical-specificity-effectiveness-Q-marker" analytical strategy	628:699	a "phytochemical-specificity-effectiveness-Q-marker" analytical strategy to improve the quality control of D. odorifera	628:746	PURPOSE Establishing a "phytochemical-specificity-effectiveness-Q-marker" analytical strategy to improve the quality control of D. odorifera.
33652358	1	7	theme	Quality	188:194	arg1	control					196:202	BACKGROUND Quality control	177:202	BACKGROUND Quality control	177:202	BACKGROUND Quality control, key for the clinical application of traditional Chinese medicines (TCMs), should be connected to the authentication and efficacy of TCMs.
33652358	7	8	theme	odorifera	1169:1177	arg1	samples					1179:1185	D. odorifera samples	1166:1185	D. odorifera samples	1166:1185	Finally, a UHPLC-HRMS method was developed and validated to quantify the selected Q-markers in D. odorifera samples.
33652358	6	9	theme	anti-inflammatory	1040:1056	arg1	ingredients					1058:1068	effective anti-inflammatory ingredients	1030:1068	effective anti-inflammatory ingredients	1030:1068	Then, lipopolysaccharide-stimulated RAW 264.7 cells were used to screen effective anti-inflammatory ingredients.
33652358	3	10	theme	clinical	548:555	arg1	efficacy					557:564	the clinical efficacy	544:564	the clinical efficacy	544:564	However, in the Chinese pharmacopeia, only the total volatile oil, which does not sufficiently reflect the clinical efficacy, is used as a quality control indicator.
33652358	10	11	theme	proinflammatory	1730:1744	arg1	interleukin-6					1781:1793	interleukin-6	1781:1793	interleukin-6	1781:1793	It revealed that the extract of D. odorifera and nine flavonoids in the noncytotoxic range could alleviated lipopolysaccharide-stimulated inflammation in RAW 264.7 cells by decreasing the production of proinflammatory mediators such as nitric oxide and interleukin-6.
33652358	10	11	theme	proinflammatory	1730:1744	arg1	oxide					1771:1775	nitric oxide	1764:1775	nitric oxide	1764:1775	It revealed that the extract of D. odorifera and nine flavonoids in the noncytotoxic range could alleviated lipopolysaccharide-stimulated inflammation in RAW 264.7 cells by decreasing the production of proinflammatory mediators such as nitric oxide and interleukin-6.
33652358	10	11	theme	proinflammatory	1730:1744	arg1	mediators					1746:1754	proinflammatory mediators	1730:1754	proinflammatory mediators such as nitric oxide and interleukin-6	1730:1793	It revealed that the extract of D. odorifera and nine flavonoids in the noncytotoxic range could alleviated lipopolysaccharide-stimulated inflammation in RAW 264.7 cells by decreasing the production of proinflammatory mediators such as nitric oxide and interleukin-6.
33652358	7	12	theme	selected	1144:1151	arg1	Q-markers					1153:1161	the selected Q-markers	1140:1161	the selected Q-markers in D. odorifera samples	1140:1185	Finally, a UHPLC-HRMS method was developed and validated to quantify the selected Q-markers in D. odorifera samples.
33652358	13	13	theme	D.	2309:2310	arg1	origins					2282:2288	origins	2282:2288	origins	2282:2288	The origins and consistency of D. odorifera could be efficiently discriminated by hierarchical cluster analysis (HCA).
33652358	13	13	theme	D.	2309:2310	arg1	consistency					2294:2304	consistency	2294:2304	consistency	2294:2304	The origins and consistency of D. odorifera could be efficiently discriminated by hierarchical cluster analysis (HCA).
33652358	12	14	from	origins	2269:2275	arg1	batches					2235:2241	10 batches	2232:2241	10 batches of samples from different origins	2232:2275	Finally, 12 Q-markers (butin, liquiritigenin, eriodictyol, melanettin, naringenin, butein, genistein, 4'-hydroxy-4-methoxydalbergione, isoliquiritigenin, 2,4-dihydroxy-5-methoxybenzophenone, medicarpin, and pinocembrin), which reflect specificity and effectiveness, were successfully quantified in 10 batches of samples from different origins.
33652358	12	14	from	origins	2269:2275	arg1	samples					2246:2252	samples	2246:2252	samples from different origins	2246:2275	Finally, 12 Q-markers (butin, liquiritigenin, eriodictyol, melanettin, naringenin, butein, genistein, 4'-hydroxy-4-methoxydalbergione, isoliquiritigenin, 2,4-dihydroxy-5-methoxybenzophenone, medicarpin, and pinocembrin), which reflect specificity and effectiveness, were successfully quantified in 10 batches of samples from different origins.
33652358	1	15	theme	clinical	217:224	arg1	application					226:236	the clinical application	213:236	the clinical application of traditional Chinese medicines (TCMs)	213:276	BACKGROUND Quality control, key for the clinical application of traditional Chinese medicines (TCMs), should be connected to the authentication and efficacy of TCMs.
33652358	3	16	used	used	570:573	arg2	oil					503:505	only the total volatile oil	479:505	only the total volatile oil	479:505	However, in the Chinese pharmacopeia, only the total volatile oil, which does not sufficiently reflect the clinical efficacy, is used as a quality control indicator.
33652358	3	16	used	used	570:573	arg2	indicator					596:604	a quality control indicator	578:604	a quality control indicator	578:604	However, in the Chinese pharmacopeia, only the total volatile oil, which does not sufficiently reflect the clinical efficacy, is used as a quality control indicator.
33652358	7	17	theme	UHPLC-HRMS	1082:1091	arg1	method					1093:1098	a UHPLC-HRMS method	1080:1098	a UHPLC-HRMS method	1080:1098	Finally, a UHPLC-HRMS method was developed and validated to quantify the selected Q-markers in D. odorifera samples.
33652358	10	18	from	extract	1549:1555	arg1	range					1613:1617	the noncytotoxic range	1596:1617	the noncytotoxic range	1596:1617	It revealed that the extract of D. odorifera and nine flavonoids in the noncytotoxic range could alleviated lipopolysaccharide-stimulated inflammation in RAW 264.7 cells by decreasing the production of proinflammatory mediators such as nitric oxide and interleukin-6.
33652358	10	19	dep	alleviated	1625:1634	arg1	could					1619:1623	could	1619:1623	could	1619:1623	It revealed that the extract of D. odorifera and nine flavonoids in the noncytotoxic range could alleviated lipopolysaccharide-stimulated inflammation in RAW 264.7 cells by decreasing the production of proinflammatory mediators such as nitric oxide and interleukin-6.
33652358	3	20	theme	Chinese	457:463	arg1	pharmacopeia					465:476	the Chinese pharmacopeia	453:476	the Chinese pharmacopeia	453:476	However, in the Chinese pharmacopeia, only the total volatile oil, which does not sufficiently reflect the clinical efficacy, is used as a quality control indicator.
33652358	11	21	theme	superior	1860:1867	arg1	activities					1887:1896	superior anti-inflammatory activities	1860:1896	superior anti-inflammatory activities	1860:1896	Notably, neoflavones, as species-specific components, exhibited superior anti-inflammatory activities among the representative compounds.
33652358	5	22	theme	pathway	784:790	arg1	analysis					792:799	biosynthetic pathway analysis	771:799	biosynthetic pathway analysis	771:799	METHODS Combined with biosynthetic pathway analysis, phytochemical compositions identified by UHPLC-Q-Orbitrap HRMS were used to build substantial phytochemical groups and further discover specific Q-markers.
33652358	3	23	theme	control	588:594	arg1	oil					503:505	only the total volatile oil	479:505	only the total volatile oil	479:505	However, in the Chinese pharmacopeia, only the total volatile oil, which does not sufficiently reflect the clinical efficacy, is used as a quality control indicator.
33652358	3	23	theme	control	588:594	arg1	indicator					596:604	a quality control indicator	578:604	a quality control indicator	578:604	However, in the Chinese pharmacopeia, only the total volatile oil, which does not sufficiently reflect the clinical efficacy, is used as a quality control indicator.
33652358	4	24	theme	quality	716:722	arg1	control					724:730	the quality control	712:730	the quality control of D. odorifera	712:746	PURPOSE Establishing a "phytochemical-specificity-effectiveness-Q-marker" analytical strategy to improve the quality control of D. odorifera.
33652358	14	25	theme	material	2526:2533	arg1	basis					2535:2539	the therapeutic material basis	2510:2539	the therapeutic material basis	2510:2539	CONCLUSION The analysis strategy that combines phytochemical analysis with anti-inflammatory screening clarified the therapeutic material basis and discovered Q-markers, which possibly offers a more comprehensive quality assessment of D. odorifera.
33652358	1	26	theme	TCMs	337:340	arg1	efficacy					325:332	efficacy	325:332	efficacy	325:332	BACKGROUND Quality control, key for the clinical application of traditional Chinese medicines (TCMs), should be connected to the authentication and efficacy of TCMs.
33652358	1	26	theme	TCMs	337:340	arg1	authentication					306:319	authentication	306:319	authentication	306:319	BACKGROUND Quality control, key for the clinical application of traditional Chinese medicines (TCMs), should be connected to the authentication and efficacy of TCMs.
33652358	10	27	from	flavonoids	1582:1591	arg1	range					1613:1617	the noncytotoxic range	1596:1617	the noncytotoxic range	1596:1617	It revealed that the extract of D. odorifera and nine flavonoids in the noncytotoxic range could alleviated lipopolysaccharide-stimulated inflammation in RAW 264.7 cells by decreasing the production of proinflammatory mediators such as nitric oxide and interleukin-6.
33652358	10	28	dep	D.	1560:1561	arg1	odorifera					1563:1571	D. odorifera	1560:1571	D. odorifera	1560:1571	It revealed that the extract of D. odorifera and nine flavonoids in the noncytotoxic range could alleviated lipopolysaccharide-stimulated inflammation in RAW 264.7 cells by decreasing the production of proinflammatory mediators such as nitric oxide and interleukin-6.
33652358	4	29	theme	D.	735:736	arg1	control					724:730	the quality control	712:730	the quality control of D. odorifera	712:746	PURPOSE Establishing a "phytochemical-specificity-effectiveness-Q-marker" analytical strategy to improve the quality control of D. odorifera.
33652358	0	30	theme	Quality	0:6	arg1	markers					8:14	Quality markers	0:14	Quality markers	0:14	Quality markers based on phytochemical analysis and anti-inflammatory screening: An integrated strategy for the quality control of Dalbergia odorifera by UHPLC-Q-Orbitrap HRMS.
33652358	4	31	dep	D.	735:736	arg1	odorifera					738:746	D. odorifera	735:746	D. odorifera	735:746	PURPOSE Establishing a "phytochemical-specificity-effectiveness-Q-marker" analytical strategy to improve the quality control of D. odorifera.
33652358	14	32	theme	phytochemical	2444:2456	arg1	analysis					2458:2465	phytochemical analysis	2444:2465	phytochemical analysis	2444:2465	CONCLUSION The analysis strategy that combines phytochemical analysis with anti-inflammatory screening clarified the therapeutic material basis and discovered Q-markers, which possibly offers a more comprehensive quality assessment of D. odorifera.
33652358	2	33	theme	inflammation-related	410:429	arg1	diseases					431:438	inflammation-related diseases	410:438	inflammation-related diseases	410:438	The heartwood of Dalbergia odorifera has been widely used to treat inflammation-related diseases.
33652358	0	34	theme	phytochemical	25:37	arg1	analysis					39:46	phytochemical analysis	25:46	phytochemical analysis	25:46	Quality markers based on phytochemical analysis and anti-inflammatory screening: An integrated strategy for the quality control of Dalbergia odorifera by UHPLC-Q-Orbitrap HRMS.
33652358	12	35	theme	butin	1957:1961	arg1	Q-markers					1946:1954	12 Q-markers	1943:1954	12 Q-markers (butin, liquiritigenin, eriodictyol, melanettin, naringenin, butein, genistein, 4'-hydroxy-4-methoxydalbergione, isoliquiritigenin, 2,4-dihydroxy-5-methoxybenzophenone, medicarpin, and pinocembrin)	1943:2152	Finally, 12 Q-markers (butin, liquiritigenin, eriodictyol, melanettin, naringenin, butein, genistein, 4'-hydroxy-4-methoxydalbergione, isoliquiritigenin, 2,4-dihydroxy-5-methoxybenzophenone, medicarpin, and pinocembrin), which reflect specificity and effectiveness, were successfully quantified in 10 batches of samples from different origins.
33652358	12	35	theme	butin	1957:1961	arg1	genistein					2025:2033	butin, liquiritigenin, eriodictyol, melanettin, naringenin, butein, genistein, 4'-hydroxy-4-methoxydalbergione, isoliquiritigenin, 2,4-dihydroxy-5-methoxybenzophenone, medicarpin, and pinocembrin	1957:2151	genistein	2025:2033	Finally, 12 Q-markers (butin, liquiritigenin, eriodictyol, melanettin, naringenin, butein, genistein, 4'-hydroxy-4-methoxydalbergione, isoliquiritigenin, 2,4-dihydroxy-5-methoxybenzophenone, medicarpin, and pinocembrin), which reflect specificity and effectiveness, were successfully quantified in 10 batches of samples from different origins.
33652358	10	36	theme	noncytotoxic	1600:1611	arg1	range					1613:1617	the noncytotoxic range	1596:1617	the noncytotoxic range	1596:1617	It revealed that the extract of D. odorifera and nine flavonoids in the noncytotoxic range could alleviated lipopolysaccharide-stimulated inflammation in RAW 264.7 cells by decreasing the production of proinflammatory mediators such as nitric oxide and interleukin-6.
33652358	8	37	dep	D.	1288:1289	arg1	odorifera					1291:1299	D. odorifera	1288:1299	D. odorifera	1288:1299	RESULTS Along the constructed biosynthetic pathways, 93 phytochemical components were identified in D. odorifera, including 7 chalcones, 13 flavanones, 21 isoflavones, 21 isoflavanones, 3 flavonols, 19 neoflavones, etc.
33652358	10	38	theme	lipopolysaccharide-stimulated	1636:1664	arg1	inflammation					1666:1677	lipopolysaccharide-stimulated inflammation	1636:1677	lipopolysaccharide-stimulated inflammation in RAW 264.7 cells	1636:1696	It revealed that the extract of D. odorifera and nine flavonoids in the noncytotoxic range could alleviated lipopolysaccharide-stimulated inflammation in RAW 264.7 cells by decreasing the production of proinflammatory mediators such as nitric oxide and interleukin-6.
33652358	1	39	theme	traditional	241:251	arg1	TCMs					272:275	TCMs	272:275	TCMs	272:275	BACKGROUND Quality control, key for the clinical application of traditional Chinese medicines (TCMs), should be connected to the authentication and efficacy of TCMs.
33652358	1	39	theme	traditional	241:251	arg1	medicines					261:269	traditional Chinese medicines	241:269	traditional Chinese medicines (TCMs)	241:276	BACKGROUND Quality control, key for the clinical application of traditional Chinese medicines (TCMs), should be connected to the authentication and efficacy of TCMs.
33652358	8	40	theme	biosynthetic	1218:1229	arg1	pathways					1231:1238	the constructed biosynthetic pathways	1202:1238	the constructed biosynthetic pathways	1202:1238	RESULTS Along the constructed biosynthetic pathways, 93 phytochemical components were identified in D. odorifera, including 7 chalcones, 13 flavanones, 21 isoflavones, 21 isoflavanones, 3 flavonols, 19 neoflavones, etc.
33652358	3	41	theme	volatile	494:501	arg1	oil					503:505	only the total volatile oil	479:505	only the total volatile oil	479:505	However, in the Chinese pharmacopeia, only the total volatile oil, which does not sufficiently reflect the clinical efficacy, is used as a quality control indicator.
33652358	3	41	theme	volatile	494:501	arg1	indicator					596:604	a quality control indicator	578:604	a quality control indicator	578:604	However, in the Chinese pharmacopeia, only the total volatile oil, which does not sufficiently reflect the clinical efficacy, is used as a quality control indicator.
33652358	1	42	theme	medicines	261:269	arg1	application					226:236	the clinical application	213:236	the clinical application of traditional Chinese medicines (TCMs)	213:276	BACKGROUND Quality control, key for the clinical application of traditional Chinese medicines (TCMs), should be connected to the authentication and efficacy of TCMs.
33652358	6	43	theme	lipopolysaccharide-stimulated	964:992	arg1	cells					1004:1008	lipopolysaccharide-stimulated RAW 264.7 cells	964:1008	lipopolysaccharide-stimulated RAW 264.7 cells	964:1008	Then, lipopolysaccharide-stimulated RAW 264.7 cells were used to screen effective anti-inflammatory ingredients.
33652358	14	44	theme	quality	2610:2616	arg1	assessment					2618:2627	a more comprehensive quality assessment	2589:2627	a more comprehensive quality assessment of D. odorifera	2589:2643	CONCLUSION The analysis strategy that combines phytochemical analysis with anti-inflammatory screening clarified the therapeutic material basis and discovered Q-markers, which possibly offers a more comprehensive quality assessment of D. odorifera.
33652358	8	45	theme	phytochemical	1244:1256	arg1	components					1258:1267	93 phytochemical components	1241:1267	93 phytochemical components	1241:1267	RESULTS Along the constructed biosynthetic pathways, 93 phytochemical components were identified in D. odorifera, including 7 chalcones, 13 flavanones, 21 isoflavones, 21 isoflavanones, 3 flavonols, 19 neoflavones, etc.
33652358	8	45	theme	phytochemical	1244:1256	arg1	RESULTS					1188:1194	RESULTS	1188:1194	RESULTS Along the constructed biosynthetic pathways	1188:1238	RESULTS Along the constructed biosynthetic pathways, 93 phytochemical components were identified in D. odorifera, including 7 chalcones, 13 flavanones, 21 isoflavones, 21 isoflavanones, 3 flavonols, 19 neoflavones, etc.
33652358	1	46	theme	BACKGROUND	177:186	arg1	control					196:202	BACKGROUND Quality control	177:202	BACKGROUND Quality control	177:202	BACKGROUND Quality control, key for the clinical application of traditional Chinese medicines (TCMs), should be connected to the authentication and efficacy of TCMs.
33652358	4	47	theme	phytochemical-specificity-effectiveness-Q-marker	631:678	arg1	strategy					692:699	a "phytochemical-specificity-effectiveness-Q-marker" analytical strategy	628:699	a "phytochemical-specificity-effectiveness-Q-marker" analytical strategy to improve the quality control of D. odorifera	628:746	PURPOSE Establishing a "phytochemical-specificity-effectiveness-Q-marker" analytical strategy to improve the quality control of D. odorifera.
33652358	7	48	theme	D.	1166:1167	arg1	samples					1179:1185	D. odorifera samples	1166:1185	D. odorifera samples	1166:1185	Finally, a UHPLC-HRMS method was developed and validated to quantify the selected Q-markers in D. odorifera samples.
33652358	4	49	theme	analytical	681:690	arg1	strategy					692:699	a "phytochemical-specificity-effectiveness-Q-marker" analytical strategy	628:699	a "phytochemical-specificity-effectiveness-Q-marker" analytical strategy to improve the quality control of D. odorifera	628:746	PURPOSE Establishing a "phytochemical-specificity-effectiveness-Q-marker" analytical strategy to improve the quality control of D. odorifera.
33652358	1	50	theme	key	205:207	arg1	control					196:202	BACKGROUND Quality control	177:202	BACKGROUND Quality control	177:202	BACKGROUND Quality control, key for the clinical application of traditional Chinese medicines (TCMs), should be connected to the authentication and efficacy of TCMs.
33652358	6	51	theme	effective	1030:1038	arg1	ingredients					1058:1068	effective anti-inflammatory ingredients	1030:1068	effective anti-inflammatory ingredients	1030:1068	Then, lipopolysaccharide-stimulated RAW 264.7 cells were used to screen effective anti-inflammatory ingredients.
33652358	10	52	theme	mediators	1746:1754	arg1	production					1716:1725	the production	1712:1725	the production of proinflammatory mediators such as nitric oxide and interleukin-6	1712:1793	It revealed that the extract of D. odorifera and nine flavonoids in the noncytotoxic range could alleviated lipopolysaccharide-stimulated inflammation in RAW 264.7 cells by decreasing the production of proinflammatory mediators such as nitric oxide and interleukin-6.
33652358	6	53	used	used	1015:1018	arg2	cells					1004:1008	lipopolysaccharide-stimulated RAW 264.7 cells	964:1008	lipopolysaccharide-stimulated RAW 264.7 cells	964:1008	Then, lipopolysaccharide-stimulated RAW 264.7 cells were used to screen effective anti-inflammatory ingredients.
33652358	5	54	theme	substantial	884:894	arg1	groups					910:915	substantial phytochemical groups	884:915	substantial phytochemical groups	884:915	METHODS Combined with biosynthetic pathway analysis, phytochemical compositions identified by UHPLC-Q-Orbitrap HRMS were used to build substantial phytochemical groups and further discover specific Q-markers.
33652358	2	55	used	used	396:399	arg2	heartwood					347:355	The heartwood	343:355	The heartwood of Dalbergia odorifera	343:378	The heartwood of Dalbergia odorifera has been widely used to treat inflammation-related diseases.
33652358	0	56	theme	UHPLC-Q-Orbitrap	154:169	arg1	HRMS					171:174	UHPLC-Q-Orbitrap HRMS	154:174	UHPLC-Q-Orbitrap HRMS	154:174	Quality markers based on phytochemical analysis and anti-inflammatory screening: An integrated strategy for the quality control of Dalbergia odorifera by UHPLC-Q-Orbitrap HRMS.
33652358	12	57	theme	samples	2246:2252	arg1	batches					2235:2241	10 batches	2232:2241	10 batches of samples from different origins	2232:2275	Finally, 12 Q-markers (butin, liquiritigenin, eriodictyol, melanettin, naringenin, butein, genistein, 4'-hydroxy-4-methoxydalbergione, isoliquiritigenin, 2,4-dihydroxy-5-methoxybenzophenone, medicarpin, and pinocembrin), which reflect specificity and effectiveness, were successfully quantified in 10 batches of samples from different origins.
33652358	12	58	theme	different	2259:2267	arg1	origins					2269:2275	different origins	2259:2275	different origins	2259:2275	Finally, 12 Q-markers (butin, liquiritigenin, eriodictyol, melanettin, naringenin, butein, genistein, 4'-hydroxy-4-methoxydalbergione, isoliquiritigenin, 2,4-dihydroxy-5-methoxybenzophenone, medicarpin, and pinocembrin), which reflect specificity and effectiveness, were successfully quantified in 10 batches of samples from different origins.
33652358	5	59	theme	biosynthetic	771:782	arg1	analysis					792:799	biosynthetic pathway analysis	771:799	biosynthetic pathway analysis	771:799	METHODS Combined with biosynthetic pathway analysis, phytochemical compositions identified by UHPLC-Q-Orbitrap HRMS were used to build substantial phytochemical groups and further discover specific Q-markers.
33652358	3	60	theme	quality	580:586	arg1	oil					503:505	only the total volatile oil	479:505	only the total volatile oil	479:505	However, in the Chinese pharmacopeia, only the total volatile oil, which does not sufficiently reflect the clinical efficacy, is used as a quality control indicator.
33652358	3	60	theme	quality	580:586	arg1	indicator					596:604	a quality control indicator	578:604	a quality control indicator	578:604	However, in the Chinese pharmacopeia, only the total volatile oil, which does not sufficiently reflect the clinical efficacy, is used as a quality control indicator.
33652358	12	61	from	batches	2235:2241	arg1	origins					2269:2275	different origins	2259:2275	different origins	2259:2275	Finally, 12 Q-markers (butin, liquiritigenin, eriodictyol, melanettin, naringenin, butein, genistein, 4'-hydroxy-4-methoxydalbergione, isoliquiritigenin, 2,4-dihydroxy-5-methoxybenzophenone, medicarpin, and pinocembrin), which reflect specificity and effectiveness, were successfully quantified in 10 batches of samples from different origins.
33652358	5	62	theme	specific	938:945	arg1	Q-markers					947:955	specific Q-markers	938:955	specific Q-markers	938:955	METHODS Combined with biosynthetic pathway analysis, phytochemical compositions identified by UHPLC-Q-Orbitrap HRMS were used to build substantial phytochemical groups and further discover specific Q-markers.
33652358	11	63	theme	anti-inflammatory	1869:1885	arg1	activities					1887:1896	superior anti-inflammatory activities	1860:1896	superior anti-inflammatory activities	1860:1896	Notably, neoflavones, as species-specific components, exhibited superior anti-inflammatory activities among the representative compounds.
33652358	0	64	theme	integrated	84:93	arg1	strategy					95:102	An integrated strategy	81:102	An integrated strategy for the quality control of Dalbergia odorifera by UHPLC-Q-Orbitrap HRMS	81:174	Quality markers based on phytochemical analysis and anti-inflammatory screening: An integrated strategy for the quality control of Dalbergia odorifera by UHPLC-Q-Orbitrap HRMS.
33652358	14	65	theme	therapeutic	2514:2524	arg1	basis					2535:2539	the therapeutic material basis	2510:2539	the therapeutic material basis	2510:2539	CONCLUSION The analysis strategy that combines phytochemical analysis with anti-inflammatory screening clarified the therapeutic material basis and discovered Q-markers, which possibly offers a more comprehensive quality assessment of D. odorifera.
33652358	14	66	dep	CONCLUSION	2397:2406	arg1	discovered					2545:2554	discovered	2545:2554	discovered Q-markers, which possibly offers a more comprehensive quality assessment of D. odorifera	2545:2643	CONCLUSION The analysis strategy that combines phytochemical analysis with anti-inflammatory screening clarified the therapeutic material basis and discovered Q-markers, which possibly offers a more comprehensive quality assessment of D. odorifera.
33652358	14	66	dep	CONCLUSION	2397:2406	arg1	clarified					2500:2508	clarified	2500:2508	clarified the therapeutic material basis	2500:2539	CONCLUSION The analysis strategy that combines phytochemical analysis with anti-inflammatory screening clarified the therapeutic material basis and discovered Q-markers, which possibly offers a more comprehensive quality assessment of D. odorifera.
33652358	1	67	dep	authentication	306:319	arg1	the					302:304	the	302:304	the	302:304	BACKGROUND Quality control, key for the clinical application of traditional Chinese medicines (TCMs), should be connected to the authentication and efficacy of TCMs.
33652358	11	68	theme	representative	1908:1921	arg1	compounds					1923:1931	the representative compounds	1904:1931	the representative compounds	1904:1931	Notably, neoflavones, as species-specific components, exhibited superior anti-inflammatory activities among the representative compounds.
33652358	0	69	theme	quality	112:118	arg1	control					120:126	the quality control	108:126	the quality control of Dalbergia odorifera by UHPLC-Q-Orbitrap HRMS	108:174	Quality markers based on phytochemical analysis and anti-inflammatory screening: An integrated strategy for the quality control of Dalbergia odorifera by UHPLC-Q-Orbitrap HRMS.
33652358	14	70	theme	anti-inflammatory	2472:2488	arg1	screening					2490:2498	anti-inflammatory screening	2472:2498	anti-inflammatory screening	2472:2498	CONCLUSION The analysis strategy that combines phytochemical analysis with anti-inflammatory screening clarified the therapeutic material basis and discovered Q-markers, which possibly offers a more comprehensive quality assessment of D. odorifera.
33652358	10	71	theme	nitric	1764:1769	arg1	oxide					1771:1775	nitric oxide	1764:1775	nitric oxide	1764:1775	It revealed that the extract of D. odorifera and nine flavonoids in the noncytotoxic range could alleviated lipopolysaccharide-stimulated inflammation in RAW 264.7 cells by decreasing the production of proinflammatory mediators such as nitric oxide and interleukin-6.
33652358	5	72	theme	UHPLC-Q-Orbitrap	843:858	arg1	HRMS					860:863	UHPLC-Q-Orbitrap HRMS	843:863	UHPLC-Q-Orbitrap HRMS	843:863	METHODS Combined with biosynthetic pathway analysis, phytochemical compositions identified by UHPLC-Q-Orbitrap HRMS were used to build substantial phytochemical groups and further discover specific Q-markers.
33652358	0	73	theme	odorifera	141:149	arg1	control					120:126	the quality control	108:126	the quality control of Dalbergia odorifera by UHPLC-Q-Orbitrap HRMS	108:174	Quality markers based on phytochemical analysis and anti-inflammatory screening: An integrated strategy for the quality control of Dalbergia odorifera by UHPLC-Q-Orbitrap HRMS.
33652358	2	74	theme	odorifera	370:378	arg1	heartwood					347:355	The heartwood	343:355	The heartwood of Dalbergia odorifera	343:378	The heartwood of Dalbergia odorifera has been widely used to treat inflammation-related diseases.
33652358	5	75	theme	phytochemical	802:814	arg1	compositions					816:827	phytochemical compositions	802:827	phytochemical compositions identified by UHPLC-Q-Orbitrap HRMS	802:863	METHODS Combined with biosynthetic pathway analysis, phytochemical compositions identified by UHPLC-Q-Orbitrap HRMS were used to build substantial phytochemical groups and further discover specific Q-markers.
33652358	10	76	theme	D.	1560:1561	arg1	extract					1549:1555	the extract	1545:1555	the extract of D. odorifera	1545:1571	It revealed that the extract of D. odorifera and nine flavonoids in the noncytotoxic range could alleviated lipopolysaccharide-stimulated inflammation in RAW 264.7 cells by decreasing the production of proinflammatory mediators such as nitric oxide and interleukin-6.
33652358	10	76	theme	D.	1560:1561	arg1	flavonoids					1582:1591	nine flavonoids	1577:1591	nine flavonoids in the noncytotoxic range	1577:1617	It revealed that the extract of D. odorifera and nine flavonoids in the noncytotoxic range could alleviated lipopolysaccharide-stimulated inflammation in RAW 264.7 cells by decreasing the production of proinflammatory mediators such as nitric oxide and interleukin-6.
33652358	13	77	theme	cluster	2373:2379	arg1	HCA					2391:2393	HCA	2391:2393	HCA	2391:2393	The origins and consistency of D. odorifera could be efficiently discriminated by hierarchical cluster analysis (HCA).
33652358	13	77	theme	cluster	2373:2379	arg1	analysis					2381:2388	hierarchical cluster analysis	2360:2388	hierarchical cluster analysis (HCA)	2360:2394	The origins and consistency of D. odorifera could be efficiently discriminated by hierarchical cluster analysis (HCA).
33652358	0	78	theme	anti-inflammatory	52:68	arg1	screening					70:78	anti-inflammatory screening	52:78	anti-inflammatory screening	52:78	Quality markers based on phytochemical analysis and anti-inflammatory screening: An integrated strategy for the quality control of Dalbergia odorifera by UHPLC-Q-Orbitrap HRMS.
33652358	5	79	used	used	870:873	arg2	compositions					816:827	phytochemical compositions	802:827	phytochemical compositions identified by UHPLC-Q-Orbitrap HRMS	802:863	METHODS Combined with biosynthetic pathway analysis, phytochemical compositions identified by UHPLC-Q-Orbitrap HRMS were used to build substantial phytochemical groups and further discover specific Q-markers.
33652358	5	79	used	used	870:873	arg2	METHODS					749:755	METHODS	749:755	METHODS Combined with biosynthetic pathway analysis	749:799	METHODS Combined with biosynthetic pathway analysis, phytochemical compositions identified by UHPLC-Q-Orbitrap HRMS were used to build substantial phytochemical groups and further discover specific Q-markers.
33652358	3	80	theme	total	488:492	arg1	oil					503:505	only the total volatile oil	479:505	only the total volatile oil	479:505	However, in the Chinese pharmacopeia, only the total volatile oil, which does not sufficiently reflect the clinical efficacy, is used as a quality control indicator.
33652358	3	80	theme	total	488:492	arg1	indicator					596:604	a quality control indicator	578:604	a quality control indicator	578:604	However, in the Chinese pharmacopeia, only the total volatile oil, which does not sufficiently reflect the clinical efficacy, is used as a quality control indicator.
33652358	8	81	theme	constructed	1206:1216	arg1	pathways					1231:1238	the constructed biosynthetic pathways	1202:1238	the constructed biosynthetic pathways	1202:1238	RESULTS Along the constructed biosynthetic pathways, 93 phytochemical components were identified in D. odorifera, including 7 chalcones, 13 flavanones, 21 isoflavones, 21 isoflavanones, 3 flavonols, 19 neoflavones, etc.
33652358	10	82	from	inflammation	1666:1677	arg1	cells					1692:1696	RAW 264.7 cells	1682:1696	RAW 264.7 cells	1682:1696	It revealed that the extract of D. odorifera and nine flavonoids in the noncytotoxic range could alleviated lipopolysaccharide-stimulated inflammation in RAW 264.7 cells by decreasing the production of proinflammatory mediators such as nitric oxide and interleukin-6.
33652358	13	83	dep	D.	2309:2310	arg1	odorifera					2312:2320	D. odorifera	2309:2320	D. odorifera	2309:2320	The origins and consistency of D. odorifera could be efficiently discriminated by hierarchical cluster analysis (HCA).
33652358	11	84	theme	species-specific	1821:1836	arg1	components					1838:1847	species-specific components	1821:1847	species-specific components	1821:1847	Notably, neoflavones, as species-specific components, exhibited superior anti-inflammatory activities among the representative compounds.
33652358	14	85	dep	D.	2632:2633	arg1	odorifera					2635:2643	D. odorifera	2632:2643	D. odorifera	2632:2643	CONCLUSION The analysis strategy that combines phytochemical analysis with anti-inflammatory screening clarified the therapeutic material basis and discovered Q-markers, which possibly offers a more comprehensive quality assessment of D. odorifera.
33652358	13	86	theme	hierarchical	2360:2371	arg1	HCA					2391:2393	HCA	2391:2393	HCA	2391:2393	The origins and consistency of D. odorifera could be efficiently discriminated by hierarchical cluster analysis (HCA).
33652358	13	86	theme	hierarchical	2360:2371	arg1	analysis					2381:2388	hierarchical cluster analysis	2360:2388	hierarchical cluster analysis (HCA)	2360:2394	The origins and consistency of D. odorifera could be efficiently discriminated by hierarchical cluster analysis (HCA).
33652358	5	87	theme	phytochemical	896:908	arg1	groups					910:915	substantial phytochemical groups	884:915	substantial phytochemical groups	884:915	METHODS Combined with biosynthetic pathway analysis, phytochemical compositions identified by UHPLC-Q-Orbitrap HRMS were used to build substantial phytochemical groups and further discover specific Q-markers.
33652358	14	88	theme	analysis	2412:2419	arg1	strategy					2421:2428	The analysis strategy	2408:2428	The analysis strategy that combines phytochemical analysis with anti-inflammatory screening	2408:2498	CONCLUSION The analysis strategy that combines phytochemical analysis with anti-inflammatory screening clarified the therapeutic material basis and discovered Q-markers, which possibly offers a more comprehensive quality assessment of D. odorifera.
33652358	14	89	theme	D.	2632:2633	arg1	assessment					2618:2627	a more comprehensive quality assessment	2589:2627	a more comprehensive quality assessment of D. odorifera	2589:2643	CONCLUSION The analysis strategy that combines phytochemical analysis with anti-inflammatory screening clarified the therapeutic material basis and discovered Q-markers, which possibly offers a more comprehensive quality assessment of D. odorifera.
33652358	1	90	theme	Chinese	253:259	arg1	TCMs					272:275	TCMs	272:275	TCMs	272:275	BACKGROUND Quality control, key for the clinical application of traditional Chinese medicines (TCMs), should be connected to the authentication and efficacy of TCMs.
33652358	1	90	theme	Chinese	253:259	arg1	medicines					261:269	traditional Chinese medicines	241:269	traditional Chinese medicines (TCMs)	241:276	BACKGROUND Quality control, key for the clinical application of traditional Chinese medicines (TCMs), should be connected to the authentication and efficacy of TCMs.
33668342	9	0	dep	g/100	1312:1316	arg1	to					1304:1305	to	1304:1305	to	1304:1305	Dietary fiber analysis revealed an increase of soluble dietary fiber from 12.6 to 17.2 g/100 g dry matter at maximum thermo-mechanical treatment.
33668342	9	1	theme	Dietary	1225:1231	arg1	analysis					1239:1246	Dietary fiber analysis	1225:1246	Dietary fiber analysis	1225:1246	Dietary fiber analysis revealed an increase of soluble dietary fiber from 12.6 to 17.2 g/100 g dry matter at maximum thermo-mechanical treatment.
33668342	0	2	from	Production	67:76	arg1	Pomace					49:54	Enzymatically Treated Apple Pomace	21:54	Enzymatically Treated Apple Pomace from Juice Production	21:76	Functionalization of Enzymatically Treated Apple Pomace from Juice Production by Extrusion Processing.
33668342	0	2	from	Production	67:76	arg1	Functionalization					0:16	Functionalization	0:16	Functionalization of Enzymatically Treated Apple Pomace from Juice Production by Extrusion Processing.	0:101	Functionalization of Enzymatically Treated Apple Pomace from Juice Production by Extrusion Processing.
33668342	8	3	theme	cell	1214:1217	arg1	wall					1219:1222	the cell wall	1210:1222	the cell wall	1210:1222	Scanning electron microscopy showed an extrusion-processing-related disruption of the cell wall.
33668342	4	4	theme	raw	584:586	arg1	material					588:595	the raw material	580:595	the raw material	580:595	To vary the range of the thermomechanical treatment, various screw speeds (200, 600, 1000 min-1), and screw configurations were applied to the raw material.
33668342	11	5	theme	side	1610:1613	arg1	chains					1615:1620	neutral side chains	1602:1620	neutral side chains of rhamnogalacturonan I	1602:1644	In short, pectin polysaccharides seem to be susceptible to thermo-mechanical stress, especially arabinans as neutral side chains of rhamnogalacturonan I.
33668342	2	6	theme	designated	289:298	arg1	applications					300:311	designated applications	289:311	designated applications	289:311	However, a modification is needed to improve the technofunctional properties according to the specific needs of designated applications.
33668342	5	7	theme	functional	620:629	arg1	analyses					631:638	Detailed chemical and functional analyses	598:638	Detailed chemical and functional analyses	598:638	Detailed chemical and functional analyses were performed to develop a comprehensive understanding of the impact of the extrusion processing on apple pomace composition and technofunctional properties as well as structures of individual polymers.
33668342	2	8	dep	specific	271:278	arg1	needs					280:284	needs	280:284	needs	280:284	However, a modification is needed to improve the technofunctional properties according to the specific needs of designated applications.
33668342	6	9	from	conditions	883:892	arg1	Extrusion					844:852	Extrusion	844:852	Extrusion at moderate thermomechanical conditions	844:892	Extrusion at moderate thermomechanical conditions increased the water absorption, swelling, and viscosity of the material.
33668342	1	10	used	used	127:130	arg2	ingredients					164:174	natural and sustainable food ingredients	135:174	natural and sustainable food ingredients	135:174	Food by-products can be used as natural and sustainable food ingredients.
33668342	1	10	used	used	127:130	arg2	by-products					108:118	Food by-products	103:118	Food by-products	103:118	Food by-products can be used as natural and sustainable food ingredients.
33668342	10	11	theme	dietary	1469:1475	arg1	fraction					1483:1490	the insoluble dietary fiber fraction	1455:1490	the insoluble dietary fiber fraction	1455:1490	Dietary fiber polysaccharide analysis demonstrated compositional changes, mainly in the insoluble dietary fiber fraction.
33668342	9	12	theme	dietary	1280:1286	arg1	fiber					1288:1292	soluble dietary fiber	1272:1292	soluble dietary fiber	1272:1292	Dietary fiber analysis revealed an increase of soluble dietary fiber from 12.6 to 17.2 g/100 g dry matter at maximum thermo-mechanical treatment.
33668342	5	13	theme	extrusion	717:725	arg1	processing					727:736	the extrusion processing	713:736	the extrusion processing	713:736	Detailed chemical and functional analyses were performed to develop a comprehensive understanding of the impact of the extrusion processing on apple pomace composition and technofunctional properties as well as structures of individual polymers.
33668342	2	14	theme	technofunctional	226:241	arg1	properties					243:252	the technofunctional properties	222:252	the technofunctional properties according to the specific needs of designated applications	222:311	However, a modification is needed to improve the technofunctional properties according to the specific needs of designated applications.
33668342	5	15	theme	polymers	834:841	arg1	structures					809:818	structures	809:818	structures of individual polymers	809:841	Detailed chemical and functional analyses were performed to develop a comprehensive understanding of the impact of the extrusion processing on apple pomace composition and technofunctional properties as well as structures of individual polymers.
33668342	5	15	theme	polymers	834:841	arg1	composition					754:764	apple pomace composition	741:764	apple pomace composition	741:764	Detailed chemical and functional analyses were performed to develop a comprehensive understanding of the impact of the extrusion processing on apple pomace composition and technofunctional properties as well as structures of individual polymers.
33668342	5	15	theme	polymers	834:841	arg1	properties					787:796	technofunctional properties	770:796	technofunctional properties	770:796	Detailed chemical and functional analyses were performed to develop a comprehensive understanding of the impact of the extrusion processing on apple pomace composition and technofunctional properties as well as structures of individual polymers.
33668342	3	16	theme	lab-scale	316:324	arg1	extruder					337:344	A lab-scale twin-screw extruder	314:344	A lab-scale twin-screw extruder	314:344	A lab-scale twin-screw extruder was used to process enzymatically treated apple pomace from commercial fruit juice production.
33668342	4	17	theme	treatment	483:491	arg1	range					453:457	the range	449:457	the range of the thermomechanical treatment	449:491	To vary the range of the thermomechanical treatment, various screw speeds (200, 600, 1000 min-1), and screw configurations were applied to the raw material.
33668342	7	18	theme	water	1079:1083	arg1	index					1096:1100	the water absorption index	1075:1100	the water absorption index	1075:1100	An increase in thermomechanical stress resulted in a higher water solubility index, but negatively affected the water absorption index, viscosity, and swelling.
33668342	7	19	theme	solubility	1033:1042	arg1	index					1044:1048	a higher water solubility index	1018:1048	a higher water solubility index	1018:1048	An increase in thermomechanical stress resulted in a higher water solubility index, but negatively affected the water absorption index, viscosity, and swelling.
33668342	6	20	theme	material	957:964	arg1	swelling					926:933	swelling	926:933	swelling	926:933	Extrusion at moderate thermomechanical conditions increased the water absorption, swelling, and viscosity of the material.
33668342	6	20	theme	material	957:964	arg1	viscosity					940:948	viscosity	940:948	viscosity	940:948	Extrusion at moderate thermomechanical conditions increased the water absorption, swelling, and viscosity of the material.
33668342	6	20	theme	material	957:964	arg1	absorption					914:923	water absorption	908:923	water absorption	908:923	Extrusion at moderate thermomechanical conditions increased the water absorption, swelling, and viscosity of the material.
33668342	11	21	theme	rhamnogalacturonan	1625:1642	arg1	chains					1615:1620	neutral side chains	1602:1620	neutral side chains of rhamnogalacturonan I	1602:1644	In short, pectin polysaccharides seem to be susceptible to thermo-mechanical stress, especially arabinans as neutral side chains of rhamnogalacturonan I.
33668342	7	22	theme	affected	1066:1073	arg1	index					1096:1100	the water absorption index	1075:1100	the water absorption index	1075:1100	An increase in thermomechanical stress resulted in a higher water solubility index, but negatively affected the water absorption index, viscosity, and swelling.
33668342	5	23	from	composition	754:764	arg1	understanding					682:694	a comprehensive understanding	666:694	a comprehensive understanding of the impact of the extrusion processing on apple pomace composition and technofunctional properties as well as structures of individual polymers	666:841	Detailed chemical and functional analyses were performed to develop a comprehensive understanding of the impact of the extrusion processing on apple pomace composition and technofunctional properties as well as structures of individual polymers.
33668342	7	24	theme	higher	1020:1025	arg1	index					1044:1048	a higher water solubility index	1018:1048	a higher water solubility index	1018:1048	An increase in thermomechanical stress resulted in a higher water solubility index, but negatively affected the water absorption index, viscosity, and swelling.
33668342	1	25	theme	natural	135:141	arg1	by-products					108:118	Food by-products	103:118	Food by-products	103:118	Food by-products can be used as natural and sustainable food ingredients.
33668342	1	25	theme	natural	135:141	arg1	ingredients					164:174	natural and sustainable food ingredients	135:174	natural and sustainable food ingredients	135:174	Food by-products can be used as natural and sustainable food ingredients.
33668342	11	26	theme	thermo-mechanical	1552:1568	arg1	stress					1570:1575	thermo-mechanical stress	1552:1575	thermo-mechanical stress	1552:1575	In short, pectin polysaccharides seem to be susceptible to thermo-mechanical stress, especially arabinans as neutral side chains of rhamnogalacturonan I.
33668342	9	27	theme	dry	1320:1322	arg1	matter					1324:1329	dry matter	1320:1329	12.6 to 17.2 g/100 g dry matter	1299:1329	Dietary fiber analysis revealed an increase of soluble dietary fiber from 12.6 to 17.2 g/100 g dry matter at maximum thermo-mechanical treatment.
33668342	1	28	theme	sustainable	147:157	arg1	by-products					108:118	Food by-products	103:118	Food by-products	103:118	Food by-products can be used as natural and sustainable food ingredients.
33668342	1	28	theme	sustainable	147:157	arg1	ingredients					164:174	natural and sustainable food ingredients	135:174	natural and sustainable food ingredients	135:174	Food by-products can be used as natural and sustainable food ingredients.
33668342	11	29	theme	neutral	1602:1608	arg1	chains					1615:1620	neutral side chains	1602:1620	neutral side chains of rhamnogalacturonan I	1602:1644	In short, pectin polysaccharides seem to be susceptible to thermo-mechanical stress, especially arabinans as neutral side chains of rhamnogalacturonan I.
33668342	0	30	theme	Extrusion	81:89	arg1	Processing					91:100	Extrusion Processing	81:100	Extrusion Processing	81:100	Functionalization of Enzymatically Treated Apple Pomace from Juice Production by Extrusion Processing.
33668342	3	31	theme	treated	380:386	arg1	pomace					394:399	enzymatically treated apple pomace	366:399	enzymatically treated apple pomace from commercial fruit juice production	366:438	A lab-scale twin-screw extruder was used to process enzymatically treated apple pomace from commercial fruit juice production.
33668342	0	32	from	Functionalization	0:16	arg1	Production					67:76	Juice Production	61:76	Juice Production	61:76	Functionalization of Enzymatically Treated Apple Pomace from Juice Production by Extrusion Processing.
33668342	9	33	theme	thermo-mechanical	1342:1358	arg1	treatment					1360:1368	maximum thermo-mechanical treatment	1334:1368	maximum thermo-mechanical treatment	1334:1368	Dietary fiber analysis revealed an increase of soluble dietary fiber from 12.6 to 17.2 g/100 g dry matter at maximum thermo-mechanical treatment.
33668342	3	34	used	used	350:353	arg2	extruder					337:344	A lab-scale twin-screw extruder	314:344	A lab-scale twin-screw extruder	314:344	A lab-scale twin-screw extruder was used to process enzymatically treated apple pomace from commercial fruit juice production.
33668342	5	35	theme	Detailed	598:605	arg1	analyses					631:638	Detailed chemical and functional analyses	598:638	Detailed chemical and functional analyses	598:638	Detailed chemical and functional analyses were performed to develop a comprehensive understanding of the impact of the extrusion processing on apple pomace composition and technofunctional properties as well as structures of individual polymers.
33668342	4	36	theme	various	494:500	arg1	speeds					508:513	various screw speeds	494:513	various screw speeds (200, 600, 1000 min-1)	494:536	To vary the range of the thermomechanical treatment, various screw speeds (200, 600, 1000 min-1), and screw configurations were applied to the raw material.
33668342	4	36	theme	various	494:500	arg1	min-1					531:535	200, 600, 1000 min-1	516:535	200, 600, 1000 min-1	516:535	To vary the range of the thermomechanical treatment, various screw speeds (200, 600, 1000 min-1), and screw configurations were applied to the raw material.
33668342	5	37	theme	pomace	747:752	arg1	composition					754:764	apple pomace composition	741:764	apple pomace composition	741:764	Detailed chemical and functional analyses were performed to develop a comprehensive understanding of the impact of the extrusion processing on apple pomace composition and technofunctional properties as well as structures of individual polymers.
33668342	3	38	theme	commercial	406:415	arg1	production					429:438	commercial fruit juice production	406:438	commercial fruit juice production	406:438	A lab-scale twin-screw extruder was used to process enzymatically treated apple pomace from commercial fruit juice production.
33668342	5	39	theme	individual	823:832	arg1	polymers					834:841	individual polymers	823:841	individual polymers	823:841	Detailed chemical and functional analyses were performed to develop a comprehensive understanding of the impact of the extrusion processing on apple pomace composition and technofunctional properties as well as structures of individual polymers.
33668342	1	40	theme	Food	103:106	arg1	by-products					108:118	Food by-products	103:118	Food by-products	103:118	Food by-products can be used as natural and sustainable food ingredients.
33668342	1	40	theme	Food	103:106	arg1	ingredients					164:174	natural and sustainable food ingredients	135:174	natural and sustainable food ingredients	135:174	Food by-products can be used as natural and sustainable food ingredients.
33668342	6	41	theme	water	908:912	arg1	absorption					914:923	water absorption	908:923	water absorption	908:923	Extrusion at moderate thermomechanical conditions increased the water absorption, swelling, and viscosity of the material.
33668342	0	42	theme	Apple	43:47	arg1	Pomace					49:54	Enzymatically Treated Apple Pomace	21:54	Enzymatically Treated Apple Pomace from Juice Production	21:76	Functionalization of Enzymatically Treated Apple Pomace from Juice Production by Extrusion Processing.
33668342	3	43	theme	juice	423:427	arg1	production					429:438	commercial fruit juice production	406:438	commercial fruit juice production	406:438	A lab-scale twin-screw extruder was used to process enzymatically treated apple pomace from commercial fruit juice production.
33668342	8	44	theme	Scanning	1128:1135	arg1	microscopy					1146:1155	Scanning electron microscopy	1128:1155	Scanning electron microscopy	1128:1155	Scanning electron microscopy showed an extrusion-processing-related disruption of the cell wall.
33668342	6	45	theme	thermomechanical	866:881	arg1	conditions					883:892	moderate thermomechanical conditions	857:892	moderate thermomechanical conditions	857:892	Extrusion at moderate thermomechanical conditions increased the water absorption, swelling, and viscosity of the material.
33668342	10	46	theme	fiber	1379:1383	arg1	analysis					1400:1407	Dietary fiber polysaccharide analysis	1371:1407	Dietary fiber polysaccharide analysis	1371:1407	Dietary fiber polysaccharide analysis demonstrated compositional changes, mainly in the insoluble dietary fiber fraction.
33668342	10	47	theme	compositional	1422:1434	arg1	changes					1436:1442	compositional changes	1422:1442	compositional changes	1422:1442	Dietary fiber polysaccharide analysis demonstrated compositional changes, mainly in the insoluble dietary fiber fraction.
33668342	5	48	from	structures	809:818	arg1	understanding					682:694	a comprehensive understanding	666:694	a comprehensive understanding of the impact of the extrusion processing on apple pomace composition and technofunctional properties as well as structures of individual polymers	666:841	Detailed chemical and functional analyses were performed to develop a comprehensive understanding of the impact of the extrusion processing on apple pomace composition and technofunctional properties as well as structures of individual polymers.
33668342	9	49	dep	g	1318:1318	arg1	matter					1324:1329	dry matter	1320:1329	12.6 to 17.2 g/100 g dry matter	1299:1329	Dietary fiber analysis revealed an increase of soluble dietary fiber from 12.6 to 17.2 g/100 g dry matter at maximum thermo-mechanical treatment.
33668342	9	50	theme	fiber	1233:1237	arg1	analysis					1239:1246	Dietary fiber analysis	1225:1246	Dietary fiber analysis	1225:1246	Dietary fiber analysis revealed an increase of soluble dietary fiber from 12.6 to 17.2 g/100 g dry matter at maximum thermo-mechanical treatment.
33668342	2	51	theme	applications	300:311	arg1	specific					271:278	specific	271:278	specific	271:278	However, a modification is needed to improve the technofunctional properties according to the specific needs of designated applications.
33668342	5	52	from	impact	703:708	arg1	structures					809:818	structures	809:818	structures of individual polymers	809:841	Detailed chemical and functional analyses were performed to develop a comprehensive understanding of the impact of the extrusion processing on apple pomace composition and technofunctional properties as well as structures of individual polymers.
33668342	5	52	from	impact	703:708	arg1	composition					754:764	apple pomace composition	741:764	apple pomace composition	741:764	Detailed chemical and functional analyses were performed to develop a comprehensive understanding of the impact of the extrusion processing on apple pomace composition and technofunctional properties as well as structures of individual polymers.
33668342	5	52	from	impact	703:708	arg1	properties					787:796	technofunctional properties	770:796	technofunctional properties	770:796	Detailed chemical and functional analyses were performed to develop a comprehensive understanding of the impact of the extrusion processing on apple pomace composition and technofunctional properties as well as structures of individual polymers.
33668342	8	53	theme	wall	1219:1222	arg1	disruption					1196:1205	an extrusion-processing-related disruption	1164:1205	an extrusion-processing-related disruption of the cell wall	1164:1222	Scanning electron microscopy showed an extrusion-processing-related disruption of the cell wall.
33668342	5	54	theme	impact	703:708	arg1	understanding					682:694	a comprehensive understanding	666:694	a comprehensive understanding of the impact of the extrusion processing on apple pomace composition and technofunctional properties as well as structures of individual polymers	666:841	Detailed chemical and functional analyses were performed to develop a comprehensive understanding of the impact of the extrusion processing on apple pomace composition and technofunctional properties as well as structures of individual polymers.
33668342	9	55	theme	soluble	1272:1278	arg1	fiber					1288:1292	soluble dietary fiber	1272:1292	soluble dietary fiber	1272:1292	Dietary fiber analysis revealed an increase of soluble dietary fiber from 12.6 to 17.2 g/100 g dry matter at maximum thermo-mechanical treatment.
33668342	4	56	theme	thermomechanical	466:481	arg1	treatment					483:491	the thermomechanical treatment	462:491	the thermomechanical treatment	462:491	To vary the range of the thermomechanical treatment, various screw speeds (200, 600, 1000 min-1), and screw configurations were applied to the raw material.
33668342	7	57	theme	absorption	1085:1094	arg1	index					1096:1100	the water absorption index	1075:1100	the water absorption index	1075:1100	An increase in thermomechanical stress resulted in a higher water solubility index, but negatively affected the water absorption index, viscosity, and swelling.
33668342	9	58	theme	fiber	1288:1292	arg1	increase					1260:1267	an increase	1257:1267	an increase of soluble dietary fiber	1257:1292	Dietary fiber analysis revealed an increase of soluble dietary fiber from 12.6 to 17.2 g/100 g dry matter at maximum thermo-mechanical treatment.
33668342	5	59	theme	comprehensive	668:680	arg1	understanding					682:694	a comprehensive understanding	666:694	a comprehensive understanding of the impact of the extrusion processing on apple pomace composition and technofunctional properties as well as structures of individual polymers	666:841	Detailed chemical and functional analyses were performed to develop a comprehensive understanding of the impact of the extrusion processing on apple pomace composition and technofunctional properties as well as structures of individual polymers.
33668342	10	60	theme	insoluble	1459:1467	arg1	fraction					1483:1490	the insoluble dietary fiber fraction	1455:1490	the insoluble dietary fiber fraction	1455:1490	Dietary fiber polysaccharide analysis demonstrated compositional changes, mainly in the insoluble dietary fiber fraction.
33668342	3	61	theme	twin-screw	326:335	arg1	extruder					337:344	A lab-scale twin-screw extruder	314:344	A lab-scale twin-screw extruder	314:344	A lab-scale twin-screw extruder was used to process enzymatically treated apple pomace from commercial fruit juice production.
33668342	10	62	theme	fiber	1477:1481	arg1	fraction					1483:1490	the insoluble dietary fiber fraction	1455:1490	the insoluble dietary fiber fraction	1455:1490	Dietary fiber polysaccharide analysis demonstrated compositional changes, mainly in the insoluble dietary fiber fraction.
33668342	5	63	from	properties	787:796	arg1	understanding					682:694	a comprehensive understanding	666:694	a comprehensive understanding of the impact of the extrusion processing on apple pomace composition and technofunctional properties as well as structures of individual polymers	666:841	Detailed chemical and functional analyses were performed to develop a comprehensive understanding of the impact of the extrusion processing on apple pomace composition and technofunctional properties as well as structures of individual polymers.
33668342	7	64	from	increase	970:977	arg1	stress					999:1004	thermomechanical stress	982:1004	thermomechanical stress	982:1004	An increase in thermomechanical stress resulted in a higher water solubility index, but negatively affected the water absorption index, viscosity, and swelling.
33668342	7	65	theme	water	1027:1031	arg1	index					1044:1048	a higher water solubility index	1018:1048	a higher water solubility index	1018:1048	An increase in thermomechanical stress resulted in a higher water solubility index, but negatively affected the water absorption index, viscosity, and swelling.
33668342	5	66	theme	technofunctional	770:785	arg1	properties					787:796	technofunctional properties	770:796	technofunctional properties	770:796	Detailed chemical and functional analyses were performed to develop a comprehensive understanding of the impact of the extrusion processing on apple pomace composition and technofunctional properties as well as structures of individual polymers.
33668342	3	67	from	production	429:438	arg1	pomace					394:399	enzymatically treated apple pomace	366:399	enzymatically treated apple pomace from commercial fruit juice production	366:438	A lab-scale twin-screw extruder was used to process enzymatically treated apple pomace from commercial fruit juice production.
33668342	1	68	theme	food	159:162	arg1	by-products					108:118	Food by-products	103:118	Food by-products	103:118	Food by-products can be used as natural and sustainable food ingredients.
33668342	1	68	theme	food	159:162	arg1	ingredients					164:174	natural and sustainable food ingredients	135:174	natural and sustainable food ingredients	135:174	Food by-products can be used as natural and sustainable food ingredients.
33668342	5	69	theme	processing	727:736	arg1	impact					703:708	the impact	699:708	the impact of the extrusion processing on apple pomace composition and technofunctional properties as well as structures of individual polymers	699:841	Detailed chemical and functional analyses were performed to develop a comprehensive understanding of the impact of the extrusion processing on apple pomace composition and technofunctional properties as well as structures of individual polymers.
33668342	9	70	theme	maximum	1334:1340	arg1	treatment					1360:1368	maximum thermo-mechanical treatment	1334:1368	maximum thermo-mechanical treatment	1334:1368	Dietary fiber analysis revealed an increase of soluble dietary fiber from 12.6 to 17.2 g/100 g dry matter at maximum thermo-mechanical treatment.
33668342	3	71	theme	apple	388:392	arg1	pomace					394:399	enzymatically treated apple pomace	366:399	enzymatically treated apple pomace from commercial fruit juice production	366:438	A lab-scale twin-screw extruder was used to process enzymatically treated apple pomace from commercial fruit juice production.
33668342	4	72	theme	screw	502:506	arg1	speeds					508:513	various screw speeds	494:513	various screw speeds (200, 600, 1000 min-1)	494:536	To vary the range of the thermomechanical treatment, various screw speeds (200, 600, 1000 min-1), and screw configurations were applied to the raw material.
33668342	4	72	theme	screw	502:506	arg1	min-1					531:535	200, 600, 1000 min-1	516:535	200, 600, 1000 min-1	516:535	To vary the range of the thermomechanical treatment, various screw speeds (200, 600, 1000 min-1), and screw configurations were applied to the raw material.
33668342	11	73	theme	pectin	1503:1508	arg1	polysaccharides					1510:1524	pectin polysaccharides	1503:1524	pectin polysaccharides	1503:1524	In short, pectin polysaccharides seem to be susceptible to thermo-mechanical stress, especially arabinans as neutral side chains of rhamnogalacturonan I.
33668342	5	74	theme	apple	741:745	arg1	composition					754:764	apple pomace composition	741:764	apple pomace composition	741:764	Detailed chemical and functional analyses were performed to develop a comprehensive understanding of the impact of the extrusion processing on apple pomace composition and technofunctional properties as well as structures of individual polymers.
33668342	5	75	theme	chemical	607:614	arg1	analyses					631:638	Detailed chemical and functional analyses	598:638	Detailed chemical and functional analyses	598:638	Detailed chemical and functional analyses were performed to develop a comprehensive understanding of the impact of the extrusion processing on apple pomace composition and technofunctional properties as well as structures of individual polymers.
33668342	3	76	theme	fruit	417:421	arg1	juice					423:427	fruit juice	417:427	commercial fruit juice production	406:438	A lab-scale twin-screw extruder was used to process enzymatically treated apple pomace from commercial fruit juice production.
33668342	4	77	theme	screw	543:547	arg1	configurations					549:562	screw configurations	543:562	screw configurations	543:562	To vary the range of the thermomechanical treatment, various screw speeds (200, 600, 1000 min-1), and screw configurations were applied to the raw material.
33668342	10	78	theme	Dietary	1371:1377	arg1	analysis					1400:1407	Dietary fiber polysaccharide analysis	1371:1407	Dietary fiber polysaccharide analysis	1371:1407	Dietary fiber polysaccharide analysis demonstrated compositional changes, mainly in the insoluble dietary fiber fraction.
33668342	0	79	theme	Treated	35:41	arg1	Pomace					49:54	Enzymatically Treated Apple Pomace	21:54	Enzymatically Treated Apple Pomace from Juice Production	21:76	Functionalization of Enzymatically Treated Apple Pomace from Juice Production by Extrusion Processing.
33668342	7	80	theme	thermomechanical	982:997	arg1	stress					999:1004	thermomechanical stress	982:1004	thermomechanical stress	982:1004	An increase in thermomechanical stress resulted in a higher water solubility index, but negatively affected the water absorption index, viscosity, and swelling.
33668342	0	81	theme	Pomace	49:54	arg1	Functionalization					0:16	Functionalization	0:16	Functionalization of Enzymatically Treated Apple Pomace from Juice Production by Extrusion Processing.	0:101	Functionalization of Enzymatically Treated Apple Pomace from Juice Production by Extrusion Processing.
33668342	0	82	theme	Juice	61:65	arg1	Production					67:76	Juice Production	61:76	Juice Production	61:76	Functionalization of Enzymatically Treated Apple Pomace from Juice Production by Extrusion Processing.
33668342	8	83	theme	electron	1137:1144	arg1	microscopy					1146:1155	Scanning electron microscopy	1128:1155	Scanning electron microscopy	1128:1155	Scanning electron microscopy showed an extrusion-processing-related disruption of the cell wall.
33668342	10	84	theme	polysaccharide	1385:1398	arg1	analysis					1400:1407	Dietary fiber polysaccharide analysis	1371:1407	Dietary fiber polysaccharide analysis	1371:1407	Dietary fiber polysaccharide analysis demonstrated compositional changes, mainly in the insoluble dietary fiber fraction.
33668342	6	85	theme	moderate	857:864	arg1	conditions					883:892	moderate thermomechanical conditions	857:892	moderate thermomechanical conditions	857:892	Extrusion at moderate thermomechanical conditions increased the water absorption, swelling, and viscosity of the material.
33668342	8	86	theme	extrusion-processing-related	1167:1194	arg1	disruption					1196:1205	an extrusion-processing-related disruption	1164:1205	an extrusion-processing-related disruption of the cell wall	1164:1222	Scanning electron microscopy showed an extrusion-processing-related disruption of the cell wall.
33668342	5	87	from	understanding	682:694	arg1	structures					809:818	structures	809:818	structures of individual polymers	809:841	Detailed chemical and functional analyses were performed to develop a comprehensive understanding of the impact of the extrusion processing on apple pomace composition and technofunctional properties as well as structures of individual polymers.
33668342	5	87	from	understanding	682:694	arg1	composition					754:764	apple pomace composition	741:764	apple pomace composition	741:764	Detailed chemical and functional analyses were performed to develop a comprehensive understanding of the impact of the extrusion processing on apple pomace composition and technofunctional properties as well as structures of individual polymers.
33668342	5	87	from	understanding	682:694	arg1	properties					787:796	technofunctional properties	770:796	technofunctional properties	770:796	Detailed chemical and functional analyses were performed to develop a comprehensive understanding of the impact of the extrusion processing on apple pomace composition and technofunctional properties as well as structures of individual polymers.
32919567	0	0	theme	Tunable	84:90	arg1	nanostructure					92:104	Tunable nanostructure	84:104	Tunable nanostructure	84:104	Synthesis of bimetallic silver-gold nanoparticle composites using a cellulose dope: Tunable nanostructure and its biological activity.
32919567	5	1	theme	atom	743:746	arg1	arrangement					748:758	NP atom arrangement	740:758	NP atom arrangement	740:758	Moreover, the correlation of bioactivity to NP atom arrangement was studied.
32919567	4	2	theme	bimetallic	680:689	arg1	NPs					691:693	the bimetallic NPs	676:693	the bimetallic NPs	676:693	Meanwhile, changing the addition order of Ag+ and AuCl4- generated different atom arrangement in the bimetallic NPs.
32919567	6	3	contain	containing	816:825	arg1	nanocomposite					802:814	the nanocomposite	798:814	the nanocomposite containing NPs with an ultrathin Ag-rich outermost shell around an Au-rich core	798:894	The result revealed that the nanocomposite containing NPs with an ultrathin Ag-rich outermost shell around an Au-rich core showed better bactericidal ability while lower cytotoxicity.
32919567	6	3	contain	containing	816:825	arg2	NPs					827:829	NPs	827:829	NPs with an ultrathin Ag-rich outermost shell around an Au-rich core	827:894	The result revealed that the nanocomposite containing NPs with an ultrathin Ag-rich outermost shell around an Au-rich core showed better bactericidal ability while lower cytotoxicity.
32919567	3	4	theme	mean	554:557	arg1	size					559:562	a mean size	552:562	a mean size of 7.9-9.7 nm	552:576	In the procedure, cellulose chain not only acts as a reducing agent but also a biocompatible support for NPs with a mean size of 7.9-9.7 nm.
32919567	6	5	theme	Au-rich	883:889	arg1	core					891:894	an Au-rich core	880:894	an Au-rich core	880:894	The result revealed that the nanocomposite containing NPs with an ultrathin Ag-rich outermost shell around an Au-rich core showed better bactericidal ability while lower cytotoxicity.
32919567	0	6	theme	biological	114:123	arg1	activity					125:132	its biological activity	110:132	its biological activity	110:132	Synthesis of bimetallic silver-gold nanoparticle composites using a cellulose dope: Tunable nanostructure and its biological activity.
32919567	4	7	theme	atom	656:659	arg1	arrangement					661:671	different atom arrangement	646:671	different atom arrangement in the bimetallic NPs	646:693	Meanwhile, changing the addition order of Ag+ and AuCl4- generated different atom arrangement in the bimetallic NPs.
32919567	5	8	theme	bioactivity	725:735	arg1	correlation					710:720	the correlation	706:720	the correlation of bioactivity to NP atom arrangement	706:758	Moreover, the correlation of bioactivity to NP atom arrangement was studied.
32919567	0	9	dep	nanostructure	92:104	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of bimetallic silver-gold nanoparticle composites using a cellulose dope	0:81	Synthesis of bimetallic silver-gold nanoparticle composites using a cellulose dope: Tunable nanostructure and its biological activity.
32919567	4	10	from	arrangement	661:671	arg1	NPs					691:693	the bimetallic NPs	676:693	the bimetallic NPs	676:693	Meanwhile, changing the addition order of Ag+ and AuCl4- generated different atom arrangement in the bimetallic NPs.
32919567	3	11	theme	cellulose	456:464	arg1	agent					500:504	a reducing agent	489:504	a reducing agent	489:504	In the procedure, cellulose chain not only acts as a reducing agent but also a biocompatible support for NPs with a mean size of 7.9-9.7 nm.
32919567	3	11	theme	cellulose	456:464	arg1	chain					466:470	cellulose chain	456:470	cellulose chain	456:470	In the procedure, cellulose chain not only acts as a reducing agent but also a biocompatible support for NPs with a mean size of 7.9-9.7 nm.
32919567	2	12	theme	unmodified	319:328	arg1	dope					330:333	its unmodified dope	315:333	its unmodified dope achieving freestanding nanocomposite decorated with bimetallic Ag-Au NPs through the one pot reaction	315:435	Here, we extend the synthesis of cellulose to its unmodified dope achieving freestanding nanocomposite decorated with bimetallic Ag-Au NPs through the one pot reaction.
32919567	1	13	theme	flexible	189:196	arg1	substrate					198:206	flexible substrate	189:206	flexible substrate	189:206	Introducing functional metal nanoparticles (NPs) into flexible substrate is being increasingly attempted to expand their application.
32919567	7	14	theme	108	1083:1085	arg1	factor					1073:1078	a high enhancement factor	1054:1078	a high enhancement factor of 108	1054:1085	In addition, the nanocomposite exhibited a sensitive SERS property for determination of R6G with a high enhancement factor of 108.
32919567	6	15	with	NPs	827:829	arg1	shell					867:871	an ultrathin Ag-rich outermost shell	836:871	an ultrathin Ag-rich outermost shell around an Au-rich core	836:894	The result revealed that the nanocomposite containing NPs with an ultrathin Ag-rich outermost shell around an Au-rich core showed better bactericidal ability while lower cytotoxicity.
32919567	4	16	theme	different	646:654	arg1	arrangement					661:671	different atom arrangement	646:671	different atom arrangement in the bimetallic NPs	646:693	Meanwhile, changing the addition order of Ag+ and AuCl4- generated different atom arrangement in the bimetallic NPs.
32919567	0	17	theme	silver-gold	24:34	arg1	composites					49:58	bimetallic silver-gold nanoparticle composites	13:58	bimetallic silver-gold nanoparticle composites using a cellulose dope	13:81	Synthesis of bimetallic silver-gold nanoparticle composites using a cellulose dope: Tunable nanostructure and its biological activity.
32919567	7	18	theme	enhancement	1061:1071	arg1	factor					1073:1078	a high enhancement factor	1054:1078	a high enhancement factor of 108	1054:1085	In addition, the nanocomposite exhibited a sensitive SERS property for determination of R6G with a high enhancement factor of 108.
32919567	0	19	theme	bimetallic	13:22	arg1	composites					49:58	bimetallic silver-gold nanoparticle composites	13:58	bimetallic silver-gold nanoparticle composites using a cellulose dope	13:81	Synthesis of bimetallic silver-gold nanoparticle composites using a cellulose dope: Tunable nanostructure and its biological activity.
32919567	6	20	theme	outermost	857:865	arg1	shell					867:871	an ultrathin Ag-rich outermost shell	836:871	an ultrathin Ag-rich outermost shell around an Au-rich core	836:894	The result revealed that the nanocomposite containing NPs with an ultrathin Ag-rich outermost shell around an Au-rich core showed better bactericidal ability while lower cytotoxicity.
32919567	2	21	theme	cellulose	302:310	arg1	synthesis					289:297	the synthesis	285:297	the synthesis of cellulose	285:310	Here, we extend the synthesis of cellulose to its unmodified dope achieving freestanding nanocomposite decorated with bimetallic Ag-Au NPs through the one pot reaction.
32919567	6	22	theme	better	903:908	arg1	ability					923:929	better bactericidal ability	903:929	better bactericidal ability	903:929	The result revealed that the nanocomposite containing NPs with an ultrathin Ag-rich outermost shell around an Au-rich core showed better bactericidal ability while lower cytotoxicity.
32919567	1	23	theme	metal	158:162	arg1	nanoparticles					164:176	functional metal nanoparticles	147:176	functional metal nanoparticles (NPs)	147:182	Introducing functional metal nanoparticles (NPs) into flexible substrate is being increasingly attempted to expand their application.
32919567	1	23	theme	metal	158:162	arg1	NPs					179:181	NPs	179:181	NPs	179:181	Introducing functional metal nanoparticles (NPs) into flexible substrate is being increasingly attempted to expand their application.
32919567	0	24	theme	composites	49:58	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of bimetallic silver-gold nanoparticle composites using a cellulose dope	0:81	Synthesis of bimetallic silver-gold nanoparticle composites using a cellulose dope: Tunable nanostructure and its biological activity.
32919567	6	25	theme	Ag-rich	849:855	arg1	shell					867:871	an ultrathin Ag-rich outermost shell	836:871	an ultrathin Ag-rich outermost shell around an Au-rich core	836:894	The result revealed that the nanocomposite containing NPs with an ultrathin Ag-rich outermost shell around an Au-rich core showed better bactericidal ability while lower cytotoxicity.
32919567	0	26	theme	nanoparticle	36:47	arg1	composites					49:58	bimetallic silver-gold nanoparticle composites	13:58	bimetallic silver-gold nanoparticle composites using a cellulose dope	13:81	Synthesis of bimetallic silver-gold nanoparticle composites using a cellulose dope: Tunable nanostructure and its biological activity.
32919567	7	27	theme	high	1056:1059	arg1	factor					1073:1078	a high enhancement factor	1054:1078	a high enhancement factor of 108	1054:1085	In addition, the nanocomposite exhibited a sensitive SERS property for determination of R6G with a high enhancement factor of 108.
32919567	6	28	theme	ultrathin	839:847	arg1	shell					867:871	an ultrathin Ag-rich outermost shell	836:871	an ultrathin Ag-rich outermost shell around an Au-rich core	836:894	The result revealed that the nanocomposite containing NPs with an ultrathin Ag-rich outermost shell around an Au-rich core showed better bactericidal ability while lower cytotoxicity.
32919567	2	29	theme	pot	424:426	arg1	reaction					428:435	the one pot reaction	416:435	the one pot reaction	416:435	Here, we extend the synthesis of cellulose to its unmodified dope achieving freestanding nanocomposite decorated with bimetallic Ag-Au NPs through the one pot reaction.
32919567	3	30	with	NPs	543:545	arg1	size					559:562	a mean size	552:562	a mean size of 7.9-9.7 nm	552:576	In the procedure, cellulose chain not only acts as a reducing agent but also a biocompatible support for NPs with a mean size of 7.9-9.7 nm.
32919567	6	31	theme	lower	937:941	arg1	cytotoxicity					943:954	lower cytotoxicity	937:954	lower cytotoxicity	937:954	The result revealed that the nanocomposite containing NPs with an ultrathin Ag-rich outermost shell around an Au-rich core showed better bactericidal ability while lower cytotoxicity.
32919567	7	32	theme	R6G	1045:1047	arg1	determination					1028:1040	determination	1028:1040	determination of R6G	1028:1047	In addition, the nanocomposite exhibited a sensitive SERS property for determination of R6G with a high enhancement factor of 108.
32919567	5	33	theme	NP	740:741	arg1	arrangement					748:758	NP atom arrangement	740:758	NP atom arrangement	740:758	Moreover, the correlation of bioactivity to NP atom arrangement was studied.
32919567	3	34	theme	reducing	491:498	arg1	agent					500:504	a reducing agent	489:504	a reducing agent	489:504	In the procedure, cellulose chain not only acts as a reducing agent but also a biocompatible support for NPs with a mean size of 7.9-9.7 nm.
32919567	3	34	theme	reducing	491:498	arg1	chain					466:470	cellulose chain	456:470	cellulose chain	456:470	In the procedure, cellulose chain not only acts as a reducing agent but also a biocompatible support for NPs with a mean size of 7.9-9.7 nm.
32919567	2	35	theme	bimetallic	387:396	arg1	NPs					404:406	bimetallic Ag-Au NPs	387:406	bimetallic Ag-Au NPs	387:406	Here, we extend the synthesis of cellulose to its unmodified dope achieving freestanding nanocomposite decorated with bimetallic Ag-Au NPs through the one pot reaction.
32919567	3	36	theme	nm	575:576	arg1	size					559:562	a mean size	552:562	a mean size of 7.9-9.7 nm	552:576	In the procedure, cellulose chain not only acts as a reducing agent but also a biocompatible support for NPs with a mean size of 7.9-9.7 nm.
32919567	7	37	theme	sensitive	1000:1008	arg1	property					1015:1022	a sensitive SERS property	998:1022	a sensitive SERS property for determination of R6G	998:1047	In addition, the nanocomposite exhibited a sensitive SERS property for determination of R6G with a high enhancement factor of 108.
32919567	2	38	theme	freestanding	345:356	arg1	nanocomposite					358:370	freestanding nanocomposite	345:370	freestanding nanocomposite decorated with bimetallic Ag-Au NPs	345:406	Here, we extend the synthesis of cellulose to its unmodified dope achieving freestanding nanocomposite decorated with bimetallic Ag-Au NPs through the one pot reaction.
32919567	7	39	theme	SERS	1010:1013	arg1	property					1015:1022	a sensitive SERS property	998:1022	a sensitive SERS property for determination of R6G	998:1047	In addition, the nanocomposite exhibited a sensitive SERS property for determination of R6G with a high enhancement factor of 108.
32919567	1	40	theme	functional	147:156	arg1	nanoparticles					164:176	functional metal nanoparticles	147:176	functional metal nanoparticles (NPs)	147:182	Introducing functional metal nanoparticles (NPs) into flexible substrate is being increasingly attempted to expand their application.
32919567	1	40	theme	functional	147:156	arg1	NPs					179:181	NPs	179:181	NPs	179:181	Introducing functional metal nanoparticles (NPs) into flexible substrate is being increasingly attempted to expand their application.
32919567	4	41	theme	Ag+	621:623	arg1	order					612:616	the addition order	599:616	the addition order of Ag+ and AuCl4-	599:634	Meanwhile, changing the addition order of Ag+ and AuCl4- generated different atom arrangement in the bimetallic NPs.
32919567	2	42	theme	Ag-Au	398:402	arg1	NPs					404:406	bimetallic Ag-Au NPs	387:406	bimetallic Ag-Au NPs	387:406	Here, we extend the synthesis of cellulose to its unmodified dope achieving freestanding nanocomposite decorated with bimetallic Ag-Au NPs through the one pot reaction.
32919567	4	43	theme	AuCl4-	629:634	arg1	order					612:616	the addition order	599:616	the addition order of Ag+ and AuCl4-	599:634	Meanwhile, changing the addition order of Ag+ and AuCl4- generated different atom arrangement in the bimetallic NPs.
32919567	4	44	theme	addition	603:610	arg1	order					612:616	the addition order	599:616	the addition order of Ag+ and AuCl4-	599:634	Meanwhile, changing the addition order of Ag+ and AuCl4- generated different atom arrangement in the bimetallic NPs.
32919567	0	45	theme	cellulose	68:76	arg1	dope					78:81	a cellulose dope	66:81	a cellulose dope	66:81	Synthesis of bimetallic silver-gold nanoparticle composites using a cellulose dope: Tunable nanostructure and its biological activity.
32919567	6	46	theme	bactericidal	910:921	arg1	ability					923:929	better bactericidal ability	903:929	better bactericidal ability	903:929	The result revealed that the nanocomposite containing NPs with an ultrathin Ag-rich outermost shell around an Au-rich core showed better bactericidal ability while lower cytotoxicity.
32919567	3	47	theme	biocompatible	517:529	arg1	support					531:537	a biocompatible support	515:537	a biocompatible support for NPs with a mean size of 7.9-9.7 nm	515:576	In the procedure, cellulose chain not only acts as a reducing agent but also a biocompatible support for NPs with a mean size of 7.9-9.7 nm.
32357197	2	0	theme	TB	515:516	arg1	LAM					537:539	LAM	537:539	LAM	537:539	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	2	0	theme	TB	515:516	arg1	lipoarabinomannan					518:534	TB lipoarabinomannan	515:534	TB lipoarabinomannan (LAM)	515:540	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	3	1	theme	unpublished	844:854	arg1	data					856:859	both published and unpublished data	825:859	both published and unpublished data	825:859	Here, we present an individual participant data meta-analysis of the diagnostic accuracy of SILVAMP-LAM in adult PLHIV, including both published and unpublished data.
32357197	11	2	theme	CD4	2126:2128	arg1	count					2130:2134	CD4 count	2126:2134	CD4 count ≤ 100 cells/μl	2126:2149	In patients with CD4 count ≤ 100 cells/μl, SILVAMP-LAM sensitivity was 87.1% (95% CI 79.3%-93.6%), compared to 56.0% (95% CI 43.9%-64.9%) for LF-LAM.
32357197	1	3	theme	traditional	366:376	arg1	diagnostics					378:388	traditional diagnostics	366:388	traditional diagnostics	366:388	BACKGROUND Tuberculosis (TB) is the most common cause of death in people living with HIV (PLHIV), yet TB often goes undiagnosed since many patients are not able to produce a sputum specimen, and traditional diagnostics are costly or unavailable.
32357197	15	4	from	technicians	2834:2844	arg1	laboratories					2858:2869	research laboratories	2849:2869	research laboratories	2849:2869	Limitations of this study include the use of biobanked, rather than fresh urine samples, and testing by skilled laboratory technicians in research laboratories, rather than at the point of care.
32357197	10	5	theme	standard	1897:1904	arg1	reference					1887:1895	a composite reference standard	1875:1904	a composite reference standard (which included patients with both microbiologically confirmed as well as clinically diagnosed TB)	1875:2003	Using a composite reference standard (which included patients with both microbiologically confirmed as well as clinically diagnosed TB), SILVAMP-LAM sensitivity was 65.8% (95% CI 55.9%-74.6%), and that of LF-LAM 31.4% (95% CI 19.1%-43.7%).
32357197	10	6	theme	composite	1877:1885	arg1	reference					1887:1895	a composite reference standard	1875:1904	a composite reference standard (which included patients with both microbiologically confirmed as well as clinically diagnosed TB)	1875:2003	Using a composite reference standard (which included patients with both microbiologically confirmed as well as clinically diagnosed TB), SILVAMP-LAM sensitivity was 65.8% (95% CI 55.9%-74.6%), and that of LF-LAM 31.4% (95% CI 19.1%-43.7%).
32357197	8	7	theme	urine	1567:1571	arg1	samples					1573:1579	Biobanked urine samples	1557:1579	Biobanked urine samples	1557:1579	Biobanked urine samples were tested, using blinded double reading, with SILVAMP-LAM and LF-LAM.
32357197	16	8	theme	TB	3007:3008	arg1	patients					3010:3017	TB patients	3007:3017	TB patients	3007:3017	CONCLUSIONS In this study, we found that SILVAMP-LAM identified a substantially higher proportion of TB patients in PLHIV than LF-LAM.
32357197	16	9	theme	higher	2986:2991	arg1	proportion					2993:3002	a substantially higher proportion	2970:3002	a substantially higher proportion of TB patients in PLHIV	2970:3026	CONCLUSIONS In this study, we found that SILVAMP-LAM identified a substantially higher proportion of TB patients in PLHIV than LF-LAM.
32357197	7	10	theme	published	1472:1480	arg1	data					1482:1485	Previously published data	1461:1485	Previously published data from inpatients	1461:1501	Previously published data from inpatients were combined with unpublished data from outpatients.
32357197	3	11	from	meta-analysis	743:755	arg1	PLHIV					808:812	adult PLHIV	802:812	adult PLHIV	802:812	Here, we present an individual participant data meta-analysis of the diagnostic accuracy of SILVAMP-LAM in adult PLHIV, including both published and unpublished data.
32357197	14	12	theme	SILVAMP-LAM	2620:2630	arg1	%					2640:2640	90.9%	2636:2640	90.9% (95% CI 87.2%-93.7%)	2636:2661	Using a microbiological reference standard, the specificity of SILVAMP-LAM was 90.9% (95% CI 87.2%-93.7%), and that of LF-LAM 95.3% (95% CI 92.2%-97.7%).
32357197	14	12	theme	SILVAMP-LAM	2620:2630	arg1	specificity					2605:2615	the specificity	2601:2615	the specificity of SILVAMP-LAM	2601:2630	Using a microbiological reference standard, the specificity of SILVAMP-LAM was 90.9% (95% CI 87.2%-93.7%), and that of LF-LAM 95.3% (95% CI 92.2%-97.7%).
32357197	18	13	theme	Further	3127:3133	arg1	work					3135:3138	Further work	3127:3138	Further work	3127:3138	Further work is needed to demonstrate accuracy when implemented as a point-of-care test.
32357197	1	14	theme	BACKGROUND	171:180	arg1	Tuberculosis					182:193	BACKGROUND Tuberculosis	171:193	BACKGROUND Tuberculosis (TB)	171:198	BACKGROUND Tuberculosis (TB) is the most common cause of death in people living with HIV (PLHIV), yet TB often goes undiagnosed since many patients are not able to produce a sputum specimen, and traditional diagnostics are costly or unavailable.
32357197	1	14	theme	BACKGROUND	171:180	arg1	cause					219:223	the most common cause	203:223	the most common cause of death in people living with HIV (PLHIV)	203:266	BACKGROUND Tuberculosis (TB) is the most common cause of death in people living with HIV (PLHIV), yet TB often goes undiagnosed since many patients are not able to produce a sputum specimen, and traditional diagnostics are costly or unavailable.
32357197	1	14	theme	BACKGROUND	171:180	arg1	TB					196:197	TB	196:197	TB	196:197	BACKGROUND Tuberculosis (TB) is the most common cause of death in people living with HIV (PLHIV), yet TB often goes undiagnosed since many patients are not able to produce a sputum specimen, and traditional diagnostics are costly or unavailable.
32357197	2	15	theme	LAM	631:633	arg1	assay					635:639	an earlier LAM assay	620:639	an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM])	620:692	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	12	16	theme	%	2381:2381	arg1	%					2396:2396	95% CI 15.8%-34.9%	2379:2396	95% CI 15.8%-34.9%	2379:2396	In patients with CD4 count 101-200 cells/μl, SILVAMP-LAM sensitivity was 62.7% (95% CI 52.4%-71.9%), compared to 25.3% (95% CI 15.8%-34.9%) for LF-LAM.
32357197	12	16	theme	%	2381:2381	arg1	%					2376:2376	25.3%	2372:2376	25.3% (95% CI 15.8%-34.9%) for LF-LAM	2372:2408	In patients with CD4 count 101-200 cells/μl, SILVAMP-LAM sensitivity was 62.7% (95% CI 52.4%-71.9%), compared to 25.3% (95% CI 15.8%-34.9%) for LF-LAM.
32357197	10	17	theme	confirmed	1959:1967	arg1	TB					2001:2002	both microbiologically confirmed as well as clinically diagnosed TB	1936:2002	both microbiologically confirmed as well as clinically diagnosed TB	1936:2002	Using a composite reference standard (which included patients with both microbiologically confirmed as well as clinically diagnosed TB), SILVAMP-LAM sensitivity was 65.8% (95% CI 55.9%-74.6%), and that of LF-LAM 31.4% (95% CI 19.1%-43.7%).
32357197	5	18	theme	CD4	1167:1169	arg1	count					1171:1175	a CD4 count	1165:1175	a CD4 count	1165:1175	Of the 1,595 PLHIV who met eligibility criteria, the majority (61%) were inpatients, median age was 37 years (IQR 30-43), 43% had a CD4 count ≤ 100 cells/μl, and 35% were receiving antiretroviral therapy.
32357197	8	19	theme	blinded	1600:1606	arg1	reading					1615:1621	blinded double reading	1600:1621	blinded double reading	1600:1621	Biobanked urine samples were tested, using blinded double reading, with SILVAMP-LAM and LF-LAM.
32357197	10	20	with	patients	1922:1929	arg1	TB					2001:2002	both microbiologically confirmed as well as clinically diagnosed TB	1936:2002	both microbiologically confirmed as well as clinically diagnosed TB	1936:2002	Using a composite reference standard (which included patients with both microbiologically confirmed as well as clinically diagnosed TB), SILVAMP-LAM sensitivity was 65.8% (95% CI 55.9%-74.6%), and that of LF-LAM 31.4% (95% CI 19.1%-43.7%).
32357197	6	21	contain	had	1264:1266	arg2	screen					1291:1296	symptom screen	1283:1296	a positive WHO symptom screen for TB	1268:1303	Most participants (94%) had a positive WHO symptom screen for TB on enrollment, and 45% were diagnosed with microbiologically confirmed TB, using mycobacterial culture or Xpert MTB/RIF testing of sputum, urine, or blood.
32357197	6	21	contain	had	1264:1266	arg1	participants					1245:1256	Most participants	1240:1256	Most participants (94%)	1240:1262	Most participants (94%) had a positive WHO symptom screen for TB on enrollment, and 45% were diagnosed with microbiologically confirmed TB, using mycobacterial culture or Xpert MTB/RIF testing of sputum, urine, or blood.
32357197	6	21	contain	had	1264:1266	arg1	%					1261:1261	94%	1259:1261	94%	1259:1261	Most participants (94%) had a positive WHO symptom screen for TB on enrollment, and 45% were diagnosed with microbiologically confirmed TB, using mycobacterial culture or Xpert MTB/RIF testing of sputum, urine, or blood.
32357197	2	22	theme	TB	469:470	arg1	LAM					472:474	Fujifilm SILVAMP TB LAM	452:474	Fujifilm SILVAMP TB LAM (SILVAMP-LAM)	452:488	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	2	22	theme	TB	469:470	arg1	assay					445:449	A novel, rapid lateral flow assay	417:449	A novel, rapid lateral flow assay	417:449	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	2	22	theme	TB	469:470	arg1	SILVAMP-LAM					477:487	SILVAMP-LAM	477:487	SILVAMP-LAM	477:487	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	6	23	theme	mycobacterial	1386:1398	arg1	culture					1400:1406	mycobacterial culture	1386:1406	mycobacterial culture	1386:1406	Most participants (94%) had a positive WHO symptom screen for TB on enrollment, and 45% were diagnosed with microbiologically confirmed TB, using mycobacterial culture or Xpert MTB/RIF testing of sputum, urine, or blood.
32357197	14	24	theme	%	2692:2692	arg1	%					2687:2687	95.3%	2683:2687	95.3% (95% CI 92.2%-97.7%)	2683:2708	Using a microbiological reference standard, the specificity of SILVAMP-LAM was 90.9% (95% CI 87.2%-93.7%), and that of LF-LAM 95.3% (95% CI 92.2%-97.7%).
32357197	14	24	theme	%	2692:2692	arg1	%					2707:2707	95% CI 92.2%-97.7%	2690:2707	95% CI 92.2%-97.7%	2690:2707	Using a microbiological reference standard, the specificity of SILVAMP-LAM was 90.9% (95% CI 87.2%-93.7%), and that of LF-LAM 95.3% (95% CI 92.2%-97.7%).
32357197	14	25	theme	microbiological	2565:2579	arg1	reference					2581:2589	a microbiological reference standard	2563:2598	a microbiological reference standard	2563:2598	Using a microbiological reference standard, the specificity of SILVAMP-LAM was 90.9% (95% CI 87.2%-93.7%), and that of LF-LAM 95.3% (95% CI 92.2%-97.7%).
32357197	10	26	theme	%	2090:2090	arg1	%					2105:2105	95% CI 19.1%-43.7%	2088:2105	95% CI 19.1%-43.7%	2088:2105	Using a composite reference standard (which included patients with both microbiologically confirmed as well as clinically diagnosed TB), SILVAMP-LAM sensitivity was 65.8% (95% CI 55.9%-74.6%), and that of LF-LAM 31.4% (95% CI 19.1%-43.7%).
32357197	10	26	theme	%	2090:2090	arg1	%					2085:2085	LF-LAM 31.4%	2074:2085	LF-LAM 31.4% (95% CI 19.1%-43.7%)	2074:2106	Using a composite reference standard (which included patients with both microbiologically confirmed as well as clinically diagnosed TB), SILVAMP-LAM sensitivity was 65.8% (95% CI 55.9%-74.6%), and that of LF-LAM 31.4% (95% CI 19.1%-43.7%).
32357197	9	27	theme	CI	1841:1842	arg1	%					1854:1854	95% CI 19.5%-50.9%	1837:1854	95% CI 19.5%-50.9%	1837:1854	Applying a microbiological reference standard for assessment of sensitivity, the overall sensitivity for TB detection was 70.7% (95% CI 59.0%-80.8%) for SILVAMP-LAM compared to 34.9% (95% CI 19.5%-50.9%) for LF-LAM.
32357197	9	27	theme	CI	1841:1842	arg1	%					1834:1834	34.9%	1830:1834	34.9% (95% CI 19.5%-50.9%) for LF-LAM	1830:1866	Applying a microbiological reference standard for assessment of sensitivity, the overall sensitivity for TB detection was 70.7% (95% CI 59.0%-80.8%) for SILVAMP-LAM compared to 34.9% (95% CI 19.5%-50.9%) for LF-LAM.
32357197	0	28	theme	in-	146:148	arg1	meta-analysis					118:130	A meta-analysis	116:130	A meta-analysis of individual in-	116:148	Diagnostic accuracy of a novel tuberculosis point-of-care urine lipoarabinomannan assay for people living with HIV: A meta-analysis of individual in- and outpatient data.
32357197	0	28	theme	in-	146:148	arg1	data					165:168	outpatient data	154:168	outpatient data	154:168	Diagnostic accuracy of a novel tuberculosis point-of-care urine lipoarabinomannan assay for people living with HIV: A meta-analysis of individual in- and outpatient data.
32357197	1	29	theme	sputum	345:350	arg1	specimen					352:359	a sputum specimen	343:359	a sputum specimen	343:359	BACKGROUND Tuberculosis (TB) is the most common cause of death in people living with HIV (PLHIV), yet TB often goes undiagnosed since many patients are not able to produce a sputum specimen, and traditional diagnostics are costly or unavailable.
32357197	6	30	theme	confirmed	1366:1374	arg1	TB					1376:1377	microbiologically confirmed TB	1348:1377	microbiologically confirmed TB	1348:1377	Most participants (94%) had a positive WHO symptom screen for TB on enrollment, and 45% were diagnosed with microbiologically confirmed TB, using mycobacterial culture or Xpert MTB/RIF testing of sputum, urine, or blood.
32357197	9	31	theme	microbiological	1664:1678	arg1	reference					1680:1688	a microbiological reference	1662:1688	a microbiological reference standard for assessment of sensitivity	1662:1727	Applying a microbiological reference standard for assessment of sensitivity, the overall sensitivity for TB detection was 70.7% (95% CI 59.0%-80.8%) for SILVAMP-LAM compared to 34.9% (95% CI 19.5%-50.9%) for LF-LAM.
32357197	9	32	theme	%	1848:1848	arg1	%					1854:1854	95% CI 19.5%-50.9%	1837:1854	95% CI 19.5%-50.9%	1837:1854	Applying a microbiological reference standard for assessment of sensitivity, the overall sensitivity for TB detection was 70.7% (95% CI 59.0%-80.8%) for SILVAMP-LAM compared to 34.9% (95% CI 19.5%-50.9%) for LF-LAM.
32357197	9	32	theme	%	1848:1848	arg1	%					1834:1834	34.9%	1830:1834	34.9% (95% CI 19.5%-50.9%) for LF-LAM	1830:1866	Applying a microbiological reference standard for assessment of sensitivity, the overall sensitivity for TB detection was 70.7% (95% CI 59.0%-80.8%) for SILVAMP-LAM compared to 34.9% (95% CI 19.5%-50.9%) for LF-LAM.
32357197	0	33	theme	outpatient	154:163	arg1	data					165:168	outpatient data	154:168	outpatient data	154:168	Diagnostic accuracy of a novel tuberculosis point-of-care urine lipoarabinomannan assay for people living with HIV: A meta-analysis of individual in- and outpatient data.
32357197	2	34	theme	lateral	432:438	arg1	LAM					472:474	Fujifilm SILVAMP TB LAM	452:474	Fujifilm SILVAMP TB LAM (SILVAMP-LAM)	452:488	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	2	34	theme	lateral	432:438	arg1	assay					445:449	A novel, rapid lateral flow assay	417:449	A novel, rapid lateral flow assay	417:449	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	2	34	theme	lateral	432:438	arg1	sensitive					578:586	sensitive	578:586	sensitive	578:586	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	12	35	theme	CD4	2276:2278	arg1	count					2280:2284	CD4 count 101-200	2276:2292	CD4 count 101-200 cells/μl	2276:2301	In patients with CD4 count 101-200 cells/μl, SILVAMP-LAM sensitivity was 62.7% (95% CI 52.4%-71.9%), compared to 25.3% (95% CI 15.8%-34.9%) for LF-LAM.
32357197	9	36	theme	standard	1690:1697	arg1	reference					1680:1688	a microbiological reference	1662:1688	a microbiological reference standard for assessment of sensitivity	1662:1727	Applying a microbiological reference standard for assessment of sensitivity, the overall sensitivity for TB detection was 70.7% (95% CI 59.0%-80.8%) for SILVAMP-LAM compared to 34.9% (95% CI 19.5%-50.9%) for LF-LAM.
32357197	11	37	theme	%	2198:2198	arg1	%					2204:2204	95% CI 79.3%-93.6%	2187:2204	95% CI 79.3%-93.6%	2187:2204	In patients with CD4 count ≤ 100 cells/μl, SILVAMP-LAM sensitivity was 87.1% (95% CI 79.3%-93.6%), compared to 56.0% (95% CI 43.9%-64.9%) for LF-LAM.
32357197	11	37	theme	%	2198:2198	arg1	%					2184:2184	87.1%	2180:2184	87.1% (95% CI 79.3%-93.6%)	2180:2205	In patients with CD4 count ≤ 100 cells/μl, SILVAMP-LAM sensitivity was 87.1% (95% CI 79.3%-93.6%), compared to 56.0% (95% CI 43.9%-64.9%) for LF-LAM.
32357197	0	38	theme	lipoarabinomannan	64:80	arg1	assay					82:86	a novel tuberculosis point-of-care urine lipoarabinomannan assay	23:86	a novel tuberculosis point-of-care urine lipoarabinomannan assay	23:86	Diagnostic accuracy of a novel tuberculosis point-of-care urine lipoarabinomannan assay for people living with HIV: A meta-analysis of individual in- and outpatient data.
32357197	13	39	dep	cells/μl	2441:2448	arg1	200					2437:2439	200	2437:2439	200	2437:2439	In those with CD4 count > 200 cells/μl, SILVAMP-LAM sensitivity was 43.9% (95% CI 34.3%-53.9%), compared to 10.9% (95% CI 5.2%-18.4%) for LF-LAM.
32357197	6	40	dep	screen	1291:1296	arg1	WHO					1279:1281	WHO	1279:1281	WHO	1279:1281	Most participants (94%) had a positive WHO symptom screen for TB on enrollment, and 45% were diagnosed with microbiologically confirmed TB, using mycobacterial culture or Xpert MTB/RIF testing of sputum, urine, or blood.
32357197	18	41	theme	point-of-care	3196:3208	arg1	test					3210:3213	a point-of-care test	3194:3213	a point-of-care test	3194:3213	Further work is needed to demonstrate accuracy when implemented as a point-of-care test.
32357197	12	42	theme	count	2280:2284	arg1	cells/μl					2294:2301	CD4 count 101-200 cells/μl	2276:2301	CD4 count 101-200 cells/μl	2276:2301	In patients with CD4 count 101-200 cells/μl, SILVAMP-LAM sensitivity was 62.7% (95% CI 52.4%-71.9%), compared to 25.3% (95% CI 15.8%-34.9%) for LF-LAM.
32357197	11	43	theme	CI	2191:2192	arg1	%					2204:2204	95% CI 79.3%-93.6%	2187:2204	95% CI 79.3%-93.6%	2187:2204	In patients with CD4 count ≤ 100 cells/μl, SILVAMP-LAM sensitivity was 87.1% (95% CI 79.3%-93.6%), compared to 56.0% (95% CI 43.9%-64.9%) for LF-LAM.
32357197	11	43	theme	CI	2191:2192	arg1	%					2184:2184	87.1%	2180:2184	87.1% (95% CI 79.3%-93.6%)	2180:2205	In patients with CD4 count ≤ 100 cells/μl, SILVAMP-LAM sensitivity was 87.1% (95% CI 79.3%-93.6%), compared to 56.0% (95% CI 43.9%-64.9%) for LF-LAM.
32357197	4	44	dep	METHODS	862:868	arg1	PLHIV					889:893	Adult PLHIV	883:893	Adult PLHIV	883:893	METHODS AND FINDINGS Adult PLHIV (≥18 years) were assessed in 5 prospective cohort studies in South Africa (3 cohorts), Vietnam, and Ghana, carried out during 2012 to 2017.
32357197	3	45	theme	participant	726:736	arg1	meta-analysis					743:755	an individual participant data meta-analysis	712:755	an individual participant data meta-analysis of the diagnostic accuracy of SILVAMP-LAM in adult PLHIV	712:812	Here, we present an individual participant data meta-analysis of the diagnostic accuracy of SILVAMP-LAM in adult PLHIV, including both published and unpublished data.
32357197	2	46	theme	Fujifilm	452:459	arg1	LAM					472:474	Fujifilm SILVAMP TB LAM	452:474	Fujifilm SILVAMP TB LAM (SILVAMP-LAM)	452:488	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	2	46	theme	Fujifilm	452:459	arg1	assay					445:449	A novel, rapid lateral flow assay	417:449	A novel, rapid lateral flow assay	417:449	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	2	46	theme	Fujifilm	452:459	arg1	SILVAMP-LAM					477:487	SILVAMP-LAM	477:487	SILVAMP-LAM	477:487	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	9	47	theme	sensitivity	1717:1727	arg1	assessment					1703:1712	assessment	1703:1712	assessment of sensitivity	1703:1727	Applying a microbiological reference standard for assessment of sensitivity, the overall sensitivity for TB detection was 70.7% (95% CI 59.0%-80.8%) for SILVAMP-LAM compared to 34.9% (95% CI 19.5%-50.9%) for LF-LAM.
32357197	1	48	theme	many	305:308	arg1	patients					310:317	many patients	305:317	many patients	305:317	BACKGROUND Tuberculosis (TB) is the most common cause of death in people living with HIV (PLHIV), yet TB often goes undiagnosed since many patients are not able to produce a sputum specimen, and traditional diagnostics are costly or unavailable.
32357197	3	49	theme	accuracy	775:782	arg1	meta-analysis					743:755	an individual participant data meta-analysis	712:755	an individual participant data meta-analysis of the diagnostic accuracy of SILVAMP-LAM in adult PLHIV	712:812	Here, we present an individual participant data meta-analysis of the diagnostic accuracy of SILVAMP-LAM in adult PLHIV, including both published and unpublished data.
32357197	14	50	dep	%	2640:2640	arg1	%					2707:2707	95% CI 92.2%-97.7%	2690:2707	95% CI 92.2%-97.7%	2690:2707	Using a microbiological reference standard, the specificity of SILVAMP-LAM was 90.9% (95% CI 87.2%-93.7%), and that of LF-LAM 95.3% (95% CI 92.2%-97.7%).
32357197	14	50	dep	%	2640:2640	arg1	%					2687:2687	95.3%	2683:2687	95.3% (95% CI 92.2%-97.7%)	2683:2708	Using a microbiological reference standard, the specificity of SILVAMP-LAM was 90.9% (95% CI 87.2%-93.7%), and that of LF-LAM 95.3% (95% CI 92.2%-97.7%).
32357197	6	51	theme	MTB/RIF	1417:1423	arg1	testing					1425:1431	Xpert MTB/RIF testing	1411:1431	Xpert MTB/RIF testing of sputum, urine, or blood	1411:1458	Most participants (94%) had a positive WHO symptom screen for TB on enrollment, and 45% were diagnosed with microbiologically confirmed TB, using mycobacterial culture or Xpert MTB/RIF testing of sputum, urine, or blood.
32357197	15	52	theme	skilled	2815:2821	arg1	technicians					2834:2844	skilled laboratory technicians	2815:2844	skilled laboratory technicians in research laboratories	2815:2869	Limitations of this study include the use of biobanked, rather than fresh urine samples, and testing by skilled laboratory technicians in research laboratories, rather than at the point of care.
32357197	12	53	theme	%	2350:2350	arg1	%					2336:2336	62.7%	2332:2336	62.7% (95% CI 52.4%-71.9%)	2332:2357	In patients with CD4 count 101-200 cells/μl, SILVAMP-LAM sensitivity was 62.7% (95% CI 52.4%-71.9%), compared to 25.3% (95% CI 15.8%-34.9%) for LF-LAM.
32357197	12	53	theme	%	2350:2350	arg1	%					2356:2356	95% CI 52.4%-71.9%	2339:2356	95% CI 52.4%-71.9%	2339:2356	In patients with CD4 count 101-200 cells/μl, SILVAMP-LAM sensitivity was 62.7% (95% CI 52.4%-71.9%), compared to 25.3% (95% CI 15.8%-34.9%) for LF-LAM.
32357197	16	54	theme	patients	3010:3017	arg1	proportion					2993:3002	a substantially higher proportion	2970:3002	a substantially higher proportion of TB patients in PLHIV	2970:3026	CONCLUSIONS In this study, we found that SILVAMP-LAM identified a substantially higher proportion of TB patients in PLHIV than LF-LAM.
32357197	10	55	theme	%	2043:2043	arg1	%					2058:2058	95% CI 55.9%-74.6%	2041:2058	95% CI 55.9%-74.6%	2041:2058	Using a composite reference standard (which included patients with both microbiologically confirmed as well as clinically diagnosed TB), SILVAMP-LAM sensitivity was 65.8% (95% CI 55.9%-74.6%), and that of LF-LAM 31.4% (95% CI 19.1%-43.7%).
32357197	10	55	theme	%	2043:2043	arg1	%					2038:2038	65.8%	2034:2038	65.8% (95% CI 55.9%-74.6%)	2034:2059	Using a composite reference standard (which included patients with both microbiologically confirmed as well as clinically diagnosed TB), SILVAMP-LAM sensitivity was 65.8% (95% CI 55.9%-74.6%), and that of LF-LAM 31.4% (95% CI 19.1%-43.7%).
32357197	14	56	theme	%	2654:2654	arg1	%					2640:2640	90.9%	2636:2640	90.9% (95% CI 87.2%-93.7%)	2636:2661	Using a microbiological reference standard, the specificity of SILVAMP-LAM was 90.9% (95% CI 87.2%-93.7%), and that of LF-LAM 95.3% (95% CI 92.2%-97.7%).
32357197	14	56	theme	%	2654:2654	arg1	%					2660:2660	95% CI 87.2%-93.7%	2643:2660	95% CI 87.2%-93.7%	2643:2660	Using a microbiological reference standard, the specificity of SILVAMP-LAM was 90.9% (95% CI 87.2%-93.7%), and that of LF-LAM 95.3% (95% CI 92.2%-97.7%).
32357197	15	57	theme	testing	2804:2810	arg1	use					2749:2751	the use	2745:2751	the use of biobanked, rather than fresh urine samples, and testing	2745:2810	Limitations of this study include the use of biobanked, rather than fresh urine samples, and testing by skilled laboratory technicians in research laboratories, rather than at the point of care.
32357197	0	58	theme	Diagnostic	0:9	arg1	accuracy					11:18	Diagnostic accuracy	0:18	Diagnostic accuracy of a novel tuberculosis point-of-care urine lipoarabinomannan assay for people living with HIV: A meta-analysis of individual in- and outpatient data.	0:169	Diagnostic accuracy of a novel tuberculosis point-of-care urine lipoarabinomannan assay for people living with HIV: A meta-analysis of individual in- and outpatient data.
32357197	13	59	theme	SILVAMP-LAM	2451:2461	arg1	sensitivity					2463:2473	SILVAMP-LAM sensitivity	2451:2473	SILVAMP-LAM sensitivity	2451:2473	In those with CD4 count > 200 cells/μl, SILVAMP-LAM sensitivity was 43.9% (95% CI 34.3%-53.9%), compared to 10.9% (95% CI 5.2%-18.4%) for LF-LAM.
32357197	3	60	theme	adult	802:806	arg1	PLHIV					808:812	adult PLHIV	802:812	adult PLHIV	802:812	Here, we present an individual participant data meta-analysis of the diagnostic accuracy of SILVAMP-LAM in adult PLHIV, including both published and unpublished data.
32357197	13	61	theme	%	2488:2488	arg1	%					2483:2483	43.9%	2479:2483	43.9% (95% CI 34.3%-53.9%)	2479:2504	In those with CD4 count > 200 cells/μl, SILVAMP-LAM sensitivity was 43.9% (95% CI 34.3%-53.9%), compared to 10.9% (95% CI 5.2%-18.4%) for LF-LAM.
32357197	13	61	theme	%	2488:2488	arg1	%					2503:2503	95% CI 34.3%-53.9%	2486:2503	95% CI 34.3%-53.9%	2486:2503	In those with CD4 count > 200 cells/μl, SILVAMP-LAM sensitivity was 43.9% (95% CI 34.3%-53.9%), compared to 10.9% (95% CI 5.2%-18.4%) for LF-LAM.
32357197	9	62	theme	TB	1758:1759	arg1	detection					1761:1769	TB detection	1758:1769	TB detection	1758:1769	Applying a microbiological reference standard for assessment of sensitivity, the overall sensitivity for TB detection was 70.7% (95% CI 59.0%-80.8%) for SILVAMP-LAM compared to 34.9% (95% CI 19.5%-50.9%) for LF-LAM.
32357197	13	63	from	%	2483:2483	arg1	those					2414:2418	those	2414:2418	those	2414:2418	In those with CD4 count > 200 cells/μl, SILVAMP-LAM sensitivity was 43.9% (95% CI 34.3%-53.9%), compared to 10.9% (95% CI 5.2%-18.4%) for LF-LAM.
32357197	15	64	theme	samples	2791:2797	arg1	use					2749:2751	the use	2745:2751	the use of biobanked, rather than fresh urine samples, and testing	2745:2810	Limitations of this study include the use of biobanked, rather than fresh urine samples, and testing by skilled laboratory technicians in research laboratories, rather than at the point of care.
32357197	3	65	theme	SILVAMP-LAM	787:797	arg1	accuracy					775:782	the diagnostic accuracy	760:782	the diagnostic accuracy of SILVAMP-LAM	760:797	Here, we present an individual participant data meta-analysis of the diagnostic accuracy of SILVAMP-LAM in adult PLHIV, including both published and unpublished data.
32357197	16	66	dep	CONCLUSIONS	2906:2916	arg1	found					2936:2940	found	2936:2940	found that SILVAMP-LAM identified a substantially higher proportion of TB patients in PLHIV than LF-LAM	2936:3038	CONCLUSIONS In this study, we found that SILVAMP-LAM identified a substantially higher proportion of TB patients in PLHIV than LF-LAM.
32357197	1	67	dep	goes	282:285	arg1	undiagnosed					287:297	undiagnosed	287:297	goes undiagnosed since many patients are not able to produce a sputum specimen, and traditional diagnostics are costly or unavailable	282:414	BACKGROUND Tuberculosis (TB) is the most common cause of death in people living with HIV (PLHIV), yet TB often goes undiagnosed since many patients are not able to produce a sputum specimen, and traditional diagnostics are costly or unavailable.
32357197	11	68	theme	CI	2231:2232	arg1	%					2224:2224	56.0%	2220:2224	56.0% (95% CI 43.9%-64.9%) for LF-LAM	2220:2256	In patients with CD4 count ≤ 100 cells/μl, SILVAMP-LAM sensitivity was 87.1% (95% CI 79.3%-93.6%), compared to 56.0% (95% CI 43.9%-64.9%) for LF-LAM.
32357197	11	68	theme	CI	2231:2232	arg1	%					2244:2244	95% CI 43.9%-64.9%	2227:2244	95% CI 43.9%-64.9%	2227:2244	In patients with CD4 count ≤ 100 cells/μl, SILVAMP-LAM sensitivity was 87.1% (95% CI 79.3%-93.6%), compared to 56.0% (95% CI 43.9%-64.9%) for LF-LAM.
32357197	12	69	theme	CI	2343:2344	arg1	%					2336:2336	62.7%	2332:2336	62.7% (95% CI 52.4%-71.9%)	2332:2357	In patients with CD4 count 101-200 cells/μl, SILVAMP-LAM sensitivity was 62.7% (95% CI 52.4%-71.9%), compared to 25.3% (95% CI 15.8%-34.9%) for LF-LAM.
32357197	12	69	theme	CI	2343:2344	arg1	%					2356:2356	95% CI 52.4%-71.9%	2339:2356	95% CI 52.4%-71.9%	2339:2356	In patients with CD4 count 101-200 cells/μl, SILVAMP-LAM sensitivity was 62.7% (95% CI 52.4%-71.9%), compared to 25.3% (95% CI 15.8%-34.9%) for LF-LAM.
32357197	0	70	theme	novel	25:29	arg1	assay					82:86	a novel tuberculosis point-of-care urine lipoarabinomannan assay	23:86	a novel tuberculosis point-of-care urine lipoarabinomannan assay	23:86	Diagnostic accuracy of a novel tuberculosis point-of-care urine lipoarabinomannan assay for people living with HIV: A meta-analysis of individual in- and outpatient data.
32357197	15	71	theme	fresh	2779:2783	arg1	samples					2791:2797	fresh urine samples	2779:2797	fresh urine samples	2779:2797	Limitations of this study include the use of biobanked, rather than fresh urine samples, and testing by skilled laboratory technicians in research laboratories, rather than at the point of care.
32357197	7	72	from	outpatients	1544:1554	arg1	data					1534:1537	unpublished data	1522:1537	unpublished data from outpatients	1522:1554	Previously published data from inpatients were combined with unpublished data from outpatients.
32357197	4	73	theme	Adult	883:887	arg1	PLHIV					889:893	Adult PLHIV	883:893	Adult PLHIV	883:893	METHODS AND FINDINGS Adult PLHIV (≥18 years) were assessed in 5 prospective cohort studies in South Africa (3 cohorts), Vietnam, and Ghana, carried out during 2012 to 2017.
32357197	6	74	theme	blood	1454:1458	arg1	testing					1425:1431	Xpert MTB/RIF testing	1411:1431	Xpert MTB/RIF testing of sputum, urine, or blood	1411:1458	Most participants (94%) had a positive WHO symptom screen for TB on enrollment, and 45% were diagnosed with microbiologically confirmed TB, using mycobacterial culture or Xpert MTB/RIF testing of sputum, urine, or blood.
32357197	6	74	theme	blood	1454:1458	arg1	culture					1400:1406	mycobacterial culture	1386:1406	mycobacterial culture	1386:1406	Most participants (94%) had a positive WHO symptom screen for TB on enrollment, and 45% were diagnosed with microbiologically confirmed TB, using mycobacterial culture or Xpert MTB/RIF testing of sputum, urine, or blood.
32357197	0	75	theme	point-of-care	44:56	arg1	assay					82:86	a novel tuberculosis point-of-care urine lipoarabinomannan assay	23:86	a novel tuberculosis point-of-care urine lipoarabinomannan assay	23:86	Diagnostic accuracy of a novel tuberculosis point-of-care urine lipoarabinomannan assay for people living with HIV: A meta-analysis of individual in- and outpatient data.
32357197	2	76	dep	novel	419:423	arg1	rapid					426:430	rapid	426:430	rapid	426:430	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	7	77	theme	unpublished	1522:1532	arg1	data					1534:1537	unpublished data	1522:1537	unpublished data from outpatients	1522:1554	Previously published data from inpatients were combined with unpublished data from outpatients.
32357197	13	78	theme	CD4	2425:2427	arg1	count					2429:2433	CD4 count	2425:2433	CD4 count > 200 cells/μl	2425:2448	In those with CD4 count > 200 cells/μl, SILVAMP-LAM sensitivity was 43.9% (95% CI 34.3%-53.9%), compared to 10.9% (95% CI 5.2%-18.4%) for LF-LAM.
32357197	4	79	theme	prospective	926:936	arg1	studies					945:951	5 prospective cohort studies	924:951	5 prospective cohort studies in South Africa (3 cohorts), Vietnam, and Ghana, carried out during 2012 to 2017	924:1032	METHODS AND FINDINGS Adult PLHIV (≥18 years) were assessed in 5 prospective cohort studies in South Africa (3 cohorts), Vietnam, and Ghana, carried out during 2012 to 2017.
32357197	4	80	dep	2017	1029:1032	arg1	to					1026:1027	to	1026:1027	to	1026:1027	METHODS AND FINDINGS Adult PLHIV (≥18 years) were assessed in 5 prospective cohort studies in South Africa (3 cohorts), Vietnam, and Ghana, carried out during 2012 to 2017.
32357197	13	81	theme	%	2536:2536	arg1	%					2542:2542	95% CI 5.2%-18.4%	2526:2542	95% CI 5.2%-18.4%	2526:2542	In those with CD4 count > 200 cells/μl, SILVAMP-LAM sensitivity was 43.9% (95% CI 34.3%-53.9%), compared to 10.9% (95% CI 5.2%-18.4%) for LF-LAM.
32357197	13	81	theme	%	2536:2536	arg1	%					2523:2523	10.9%	2519:2523	10.9% (95% CI 5.2%-18.4%) for LF-LAM	2519:2554	In those with CD4 count > 200 cells/μl, SILVAMP-LAM sensitivity was 43.9% (95% CI 34.3%-53.9%), compared to 10.9% (95% CI 5.2%-18.4%) for LF-LAM.
32357197	17	82	theme	SILVAMP-LAM	3060:3070	arg1	sensitivity					3045:3055	The sensitivity	3041:3055	The sensitivity of SILVAMP-LAM	3041:3070	The sensitivity of SILVAMP-LAM was highest in patients with CD4 count ≤ 100 cells/μl.
32357197	17	82	theme	SILVAMP-LAM	3060:3070	arg1	highest					3076:3082	highest	3076:3082	highest	3076:3082	The sensitivity of SILVAMP-LAM was highest in patients with CD4 count ≤ 100 cells/μl.
32357197	12	83	from	%	2336:2336	arg1	patients					2262:2269	patients	2262:2269	patients with CD4 count 101-200 cells/μl	2262:2301	In patients with CD4 count 101-200 cells/μl, SILVAMP-LAM sensitivity was 62.7% (95% CI 52.4%-71.9%), compared to 25.3% (95% CI 15.8%-34.9%) for LF-LAM.
32357197	2	84	dep	assay	635:639	arg1	Alere					642:646	Alere	642:646	Alere	642:646	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	2	85	theme	assay	678:682	arg1	[LF-LAM					684:690	lateral flow assay [LF-LAM	665:690	Alere Determine TB LAM lateral flow assay [LF-LAM	642:690	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	9	86	theme	CI	1786:1787	arg1	%					1779:1779	70.7%	1775:1779	70.7% (95% CI 59.0%-80.8%) for SILVAMP-LAM	1775:1816	Applying a microbiological reference standard for assessment of sensitivity, the overall sensitivity for TB detection was 70.7% (95% CI 59.0%-80.8%) for SILVAMP-LAM compared to 34.9% (95% CI 19.5%-50.9%) for LF-LAM.
32357197	9	86	theme	CI	1786:1787	arg1	%					1799:1799	95% CI 59.0%-80.8%	1782:1799	95% CI 59.0%-80.8%	1782:1799	Applying a microbiological reference standard for assessment of sensitivity, the overall sensitivity for TB detection was 70.7% (95% CI 59.0%-80.8%) for SILVAMP-LAM compared to 34.9% (95% CI 19.5%-50.9%) for LF-LAM.
32357197	11	87	theme	%	2238:2238	arg1	%					2224:2224	56.0%	2220:2224	56.0% (95% CI 43.9%-64.9%) for LF-LAM	2220:2256	In patients with CD4 count ≤ 100 cells/μl, SILVAMP-LAM sensitivity was 87.1% (95% CI 79.3%-93.6%), compared to 56.0% (95% CI 43.9%-64.9%) for LF-LAM.
32357197	11	87	theme	%	2238:2238	arg1	%					2244:2244	95% CI 43.9%-64.9%	2227:2244	95% CI 43.9%-64.9%	2227:2244	In patients with CD4 count ≤ 100 cells/μl, SILVAMP-LAM sensitivity was 87.1% (95% CI 79.3%-93.6%), compared to 56.0% (95% CI 43.9%-64.9%) for LF-LAM.
32357197	5	88	theme	1,595	1042:1046	arg1	PLHIV					1048:1052	the 1,595 PLHIV	1038:1052	the 1,595 PLHIV who met eligibility criteria	1038:1081	Of the 1,595 PLHIV who met eligibility criteria, the majority (61%) were inpatients, median age was 37 years (IQR 30-43), 43% had a CD4 count ≤ 100 cells/μl, and 35% were receiving antiretroviral therapy.
32357197	2	89	dep	Alere	642:646	arg1	[LF-LAM					684:690	lateral flow assay [LF-LAM	665:690	Alere Determine TB LAM lateral flow assay [LF-LAM	642:690	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	2	89	dep	Alere	642:646	arg1	Determine					648:656	Determine	648:656	Determine TB LAM	648:663	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	14	90	theme	CI	2647:2648	arg1	%					2640:2640	90.9%	2636:2640	90.9% (95% CI 87.2%-93.7%)	2636:2661	Using a microbiological reference standard, the specificity of SILVAMP-LAM was 90.9% (95% CI 87.2%-93.7%), and that of LF-LAM 95.3% (95% CI 92.2%-97.7%).
32357197	14	90	theme	CI	2647:2648	arg1	%					2660:2660	95% CI 87.2%-93.7%	2643:2660	95% CI 87.2%-93.7%	2643:2660	Using a microbiological reference standard, the specificity of SILVAMP-LAM was 90.9% (95% CI 87.2%-93.7%), and that of LF-LAM 95.3% (95% CI 92.2%-97.7%).
32357197	9	91	theme	%	1793:1793	arg1	%					1779:1779	70.7%	1775:1779	70.7% (95% CI 59.0%-80.8%) for SILVAMP-LAM	1775:1816	Applying a microbiological reference standard for assessment of sensitivity, the overall sensitivity for TB detection was 70.7% (95% CI 59.0%-80.8%) for SILVAMP-LAM compared to 34.9% (95% CI 19.5%-50.9%) for LF-LAM.
32357197	9	91	theme	%	1793:1793	arg1	%					1799:1799	95% CI 59.0%-80.8%	1782:1799	95% CI 59.0%-80.8%	1782:1799	Applying a microbiological reference standard for assessment of sensitivity, the overall sensitivity for TB detection was 70.7% (95% CI 59.0%-80.8%) for SILVAMP-LAM compared to 34.9% (95% CI 19.5%-50.9%) for LF-LAM.
32357197	8	92	theme	Biobanked	1557:1565	arg1	samples					1573:1579	Biobanked urine samples	1557:1579	Biobanked urine samples	1557:1579	Biobanked urine samples were tested, using blinded double reading, with SILVAMP-LAM and LF-LAM.
32357197	12	93	theme	SILVAMP-LAM	2304:2314	arg1	sensitivity					2316:2326	SILVAMP-LAM sensitivity	2304:2326	SILVAMP-LAM sensitivity	2304:2326	In patients with CD4 count 101-200 cells/μl, SILVAMP-LAM sensitivity was 62.7% (95% CI 52.4%-71.9%), compared to 25.3% (95% CI 15.8%-34.9%) for LF-LAM.
32357197	1	94	theme	death	228:232	arg1	cause					219:223	the most common cause	203:223	the most common cause of death in people living with HIV (PLHIV)	203:266	BACKGROUND Tuberculosis (TB) is the most common cause of death in people living with HIV (PLHIV), yet TB often goes undiagnosed since many patients are not able to produce a sputum specimen, and traditional diagnostics are costly or unavailable.
32357197	1	94	theme	death	228:232	arg1	Tuberculosis					182:193	BACKGROUND Tuberculosis	171:193	BACKGROUND Tuberculosis (TB)	171:198	BACKGROUND Tuberculosis (TB) is the most common cause of death in people living with HIV (PLHIV), yet TB often goes undiagnosed since many patients are not able to produce a sputum specimen, and traditional diagnostics are costly or unavailable.
32357197	12	95	theme	%	2341:2341	arg1	%					2336:2336	62.7%	2332:2336	62.7% (95% CI 52.4%-71.9%)	2332:2357	In patients with CD4 count 101-200 cells/μl, SILVAMP-LAM sensitivity was 62.7% (95% CI 52.4%-71.9%), compared to 25.3% (95% CI 15.8%-34.9%) for LF-LAM.
32357197	12	95	theme	%	2341:2341	arg1	%					2356:2356	95% CI 52.4%-71.9%	2339:2356	95% CI 52.4%-71.9%	2339:2356	In patients with CD4 count 101-200 cells/μl, SILVAMP-LAM sensitivity was 62.7% (95% CI 52.4%-71.9%), compared to 25.3% (95% CI 15.8%-34.9%) for LF-LAM.
32357197	13	96	dep	count	2429:2433	arg1	cells/μl					2441:2448	cells/μl	2441:2448	cells/μl	2441:2448	In those with CD4 count > 200 cells/μl, SILVAMP-LAM sensitivity was 43.9% (95% CI 34.3%-53.9%), compared to 10.9% (95% CI 5.2%-18.4%) for LF-LAM.
32357197	2	97	attach	presence	503:510	arg2	LAM					537:539	LAM	537:539	LAM	537:539	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	2	97	attach	presence	503:510	arg2	lipoarabinomannan					518:534	TB lipoarabinomannan	515:534	TB lipoarabinomannan (LAM)	515:540	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	2	97	attach	presence	503:510	arg1	urine					545:549	urine	545:549	urine	545:549	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	11	98	from	%	2184:2184	arg1	patients					2112:2119	patients	2112:2119	patients with CD4 count ≤ 100 cells/μl	2112:2149	In patients with CD4 count ≤ 100 cells/μl, SILVAMP-LAM sensitivity was 87.1% (95% CI 79.3%-93.6%), compared to 56.0% (95% CI 43.9%-64.9%) for LF-LAM.
32357197	14	99	theme	%	2645:2645	arg1	%					2640:2640	90.9%	2636:2640	90.9% (95% CI 87.2%-93.7%)	2636:2661	Using a microbiological reference standard, the specificity of SILVAMP-LAM was 90.9% (95% CI 87.2%-93.7%), and that of LF-LAM 95.3% (95% CI 92.2%-97.7%).
32357197	14	99	theme	%	2645:2645	arg1	%					2660:2660	95% CI 87.2%-93.7%	2643:2660	95% CI 87.2%-93.7%	2643:2660	Using a microbiological reference standard, the specificity of SILVAMP-LAM was 90.9% (95% CI 87.2%-93.7%), and that of LF-LAM 95.3% (95% CI 92.2%-97.7%).
32357197	10	100	theme	diagnosed	1991:1999	arg1	TB					2001:2002	both microbiologically confirmed as well as clinically diagnosed TB	1936:2002	both microbiologically confirmed as well as clinically diagnosed TB	1936:2002	Using a composite reference standard (which included patients with both microbiologically confirmed as well as clinically diagnosed TB), SILVAMP-LAM sensitivity was 65.8% (95% CI 55.9%-74.6%), and that of LF-LAM 31.4% (95% CI 19.1%-43.7%).
32357197	12	101	theme	CI	2383:2384	arg1	%					2396:2396	95% CI 15.8%-34.9%	2379:2396	95% CI 15.8%-34.9%	2379:2396	In patients with CD4 count 101-200 cells/μl, SILVAMP-LAM sensitivity was 62.7% (95% CI 52.4%-71.9%), compared to 25.3% (95% CI 15.8%-34.9%) for LF-LAM.
32357197	12	101	theme	CI	2383:2384	arg1	%					2376:2376	25.3%	2372:2376	25.3% (95% CI 15.8%-34.9%) for LF-LAM	2372:2408	In patients with CD4 count 101-200 cells/μl, SILVAMP-LAM sensitivity was 62.7% (95% CI 52.4%-71.9%), compared to 25.3% (95% CI 15.8%-34.9%) for LF-LAM.
32357197	13	102	theme	CI	2530:2531	arg1	%					2542:2542	95% CI 5.2%-18.4%	2526:2542	95% CI 5.2%-18.4%	2526:2542	In those with CD4 count > 200 cells/μl, SILVAMP-LAM sensitivity was 43.9% (95% CI 34.3%-53.9%), compared to 10.9% (95% CI 5.2%-18.4%) for LF-LAM.
32357197	13	102	theme	CI	2530:2531	arg1	%					2523:2523	10.9%	2519:2523	10.9% (95% CI 5.2%-18.4%) for LF-LAM	2519:2554	In those with CD4 count > 200 cells/μl, SILVAMP-LAM sensitivity was 43.9% (95% CI 34.3%-53.9%), compared to 10.9% (95% CI 5.2%-18.4%) for LF-LAM.
32357197	2	103	theme	lipoarabinomannan	518:534	arg1	presence					503:510	the presence	499:510	the presence of TB lipoarabinomannan (LAM) in urine	499:549	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	2	104	theme	lateral	665:671	arg1	[LF-LAM					684:690	lateral flow assay [LF-LAM	665:690	Alere Determine TB LAM lateral flow assay [LF-LAM	642:690	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	2	105	from	TB	603:604	arg1	PLHIV					609:613	PLHIV	609:613	PLHIV	609:613	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	8	106	theme	double	1608:1613	arg1	reading					1615:1621	blinded double reading	1600:1621	blinded double reading	1600:1621	Biobanked urine samples were tested, using blinded double reading, with SILVAMP-LAM and LF-LAM.
32357197	16	107	from	proportion	2993:3002	arg1	PLHIV					3022:3026	PLHIV	3022:3026	PLHIV	3022:3026	CONCLUSIONS In this study, we found that SILVAMP-LAM identified a substantially higher proportion of TB patients in PLHIV than LF-LAM.
32357197	12	108	theme	%	2390:2390	arg1	%					2396:2396	95% CI 15.8%-34.9%	2379:2396	95% CI 15.8%-34.9%	2379:2396	In patients with CD4 count 101-200 cells/μl, SILVAMP-LAM sensitivity was 62.7% (95% CI 52.4%-71.9%), compared to 25.3% (95% CI 15.8%-34.9%) for LF-LAM.
32357197	12	108	theme	%	2390:2390	arg1	%					2376:2376	25.3%	2372:2376	25.3% (95% CI 15.8%-34.9%) for LF-LAM	2372:2408	In patients with CD4 count 101-200 cells/μl, SILVAMP-LAM sensitivity was 62.7% (95% CI 52.4%-71.9%), compared to 25.3% (95% CI 15.8%-34.9%) for LF-LAM.
32357197	6	109	theme	Most	1240:1243	arg1	participants					1245:1256	Most participants	1240:1256	Most participants (94%)	1240:1262	Most participants (94%) had a positive WHO symptom screen for TB on enrollment, and 45% were diagnosed with microbiologically confirmed TB, using mycobacterial culture or Xpert MTB/RIF testing of sputum, urine, or blood.
32357197	6	109	theme	Most	1240:1243	arg1	%					1261:1261	94%	1259:1261	94%	1259:1261	Most participants (94%) had a positive WHO symptom screen for TB on enrollment, and 45% were diagnosed with microbiologically confirmed TB, using mycobacterial culture or Xpert MTB/RIF testing of sputum, urine, or blood.
32357197	2	110	theme	TB	658:659	arg1	LAM					661:663	TB LAM	658:663	TB LAM	658:663	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	10	111	theme	LF-LAM	2074:2079	arg1	%					2085:2085	LF-LAM 31.4%	2074:2085	LF-LAM 31.4% (95% CI 19.1%-43.7%)	2074:2106	Using a composite reference standard (which included patients with both microbiologically confirmed as well as clinically diagnosed TB), SILVAMP-LAM sensitivity was 65.8% (95% CI 55.9%-74.6%), and that of LF-LAM 31.4% (95% CI 19.1%-43.7%).
32357197	10	111	theme	LF-LAM	2074:2079	arg1	%					2105:2105	95% CI 19.1%-43.7%	2088:2105	95% CI 19.1%-43.7%	2088:2105	Using a composite reference standard (which included patients with both microbiologically confirmed as well as clinically diagnosed TB), SILVAMP-LAM sensitivity was 65.8% (95% CI 55.9%-74.6%), and that of LF-LAM 31.4% (95% CI 19.1%-43.7%).
32357197	0	112	theme	individual	135:144	arg1	in-					146:148	individual in-	135:148	individual in-	135:148	Diagnostic accuracy of a novel tuberculosis point-of-care urine lipoarabinomannan assay for people living with HIV: A meta-analysis of individual in- and outpatient data.
32357197	14	113	theme	%	2701:2701	arg1	%					2687:2687	95.3%	2683:2687	95.3% (95% CI 92.2%-97.7%)	2683:2708	Using a microbiological reference standard, the specificity of SILVAMP-LAM was 90.9% (95% CI 87.2%-93.7%), and that of LF-LAM 95.3% (95% CI 92.2%-97.7%).
32357197	14	113	theme	%	2701:2701	arg1	%					2707:2707	95% CI 92.2%-97.7%	2690:2707	95% CI 92.2%-97.7%	2690:2707	Using a microbiological reference standard, the specificity of SILVAMP-LAM was 90.9% (95% CI 87.2%-93.7%), and that of LF-LAM 95.3% (95% CI 92.2%-97.7%).
32357197	2	114	theme	SILVAMP	461:467	arg1	LAM					472:474	Fujifilm SILVAMP TB LAM	452:474	Fujifilm SILVAMP TB LAM (SILVAMP-LAM)	452:488	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	2	114	theme	SILVAMP	461:467	arg1	assay					445:449	A novel, rapid lateral flow assay	417:449	A novel, rapid lateral flow assay	417:449	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	2	114	theme	SILVAMP	461:467	arg1	SILVAMP-LAM					477:487	SILVAMP-LAM	477:487	SILVAMP-LAM	477:487	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	13	115	theme	%	2497:2497	arg1	%					2483:2483	43.9%	2479:2483	43.9% (95% CI 34.3%-53.9%)	2479:2504	In those with CD4 count > 200 cells/μl, SILVAMP-LAM sensitivity was 43.9% (95% CI 34.3%-53.9%), compared to 10.9% (95% CI 5.2%-18.4%) for LF-LAM.
32357197	13	115	theme	%	2497:2497	arg1	%					2503:2503	95% CI 34.3%-53.9%	2486:2503	95% CI 34.3%-53.9%	2486:2503	In those with CD4 count > 200 cells/μl, SILVAMP-LAM sensitivity was 43.9% (95% CI 34.3%-53.9%), compared to 10.9% (95% CI 5.2%-18.4%) for LF-LAM.
32357197	15	116	theme	care	2900:2903	arg1	point					2891:2895	the point	2887:2895	the point of care	2887:2903	Limitations of this study include the use of biobanked, rather than fresh urine samples, and testing by skilled laboratory technicians in research laboratories, rather than at the point of care.
32357197	9	117	theme	%	1839:1839	arg1	%					1854:1854	95% CI 19.5%-50.9%	1837:1854	95% CI 19.5%-50.9%	1837:1854	Applying a microbiological reference standard for assessment of sensitivity, the overall sensitivity for TB detection was 70.7% (95% CI 59.0%-80.8%) for SILVAMP-LAM compared to 34.9% (95% CI 19.5%-50.9%) for LF-LAM.
32357197	9	117	theme	%	1839:1839	arg1	%					1834:1834	34.9%	1830:1834	34.9% (95% CI 19.5%-50.9%) for LF-LAM	1830:1866	Applying a microbiological reference standard for assessment of sensitivity, the overall sensitivity for TB detection was 70.7% (95% CI 59.0%-80.8%) for SILVAMP-LAM compared to 34.9% (95% CI 19.5%-50.9%) for LF-LAM.
32357197	1	118	from	cause	219:223	arg1	people					237:242	people	237:242	people living with HIV (PLHIV)	237:266	BACKGROUND Tuberculosis (TB) is the most common cause of death in people living with HIV (PLHIV), yet TB often goes undiagnosed since many patients are not able to produce a sputum specimen, and traditional diagnostics are costly or unavailable.
32357197	4	119	from	studies	945:951	arg1	Africa					962:967	Africa	962:967	Africa	962:967	METHODS AND FINDINGS Adult PLHIV (≥18 years) were assessed in 5 prospective cohort studies in South Africa (3 cohorts), Vietnam, and Ghana, carried out during 2012 to 2017.
32357197	4	119	from	studies	945:951	arg1	cohorts					972:978	3 cohorts	970:978	3 cohorts	970:978	METHODS AND FINDINGS Adult PLHIV (≥18 years) were assessed in 5 prospective cohort studies in South Africa (3 cohorts), Vietnam, and Ghana, carried out during 2012 to 2017.
32357197	4	119	from	studies	945:951	arg1	Vietnam					982:988	Vietnam	982:988	Vietnam	982:988	METHODS AND FINDINGS Adult PLHIV (≥18 years) were assessed in 5 prospective cohort studies in South Africa (3 cohorts), Vietnam, and Ghana, carried out during 2012 to 2017.
32357197	4	119	from	studies	945:951	arg1	Ghana					995:999	Ghana	995:999	Ghana	995:999	METHODS AND FINDINGS Adult PLHIV (≥18 years) were assessed in 5 prospective cohort studies in South Africa (3 cohorts), Vietnam, and Ghana, carried out during 2012 to 2017.
32357197	11	120	theme	%	2189:2189	arg1	%					2204:2204	95% CI 79.3%-93.6%	2187:2204	95% CI 79.3%-93.6%	2187:2204	In patients with CD4 count ≤ 100 cells/μl, SILVAMP-LAM sensitivity was 87.1% (95% CI 79.3%-93.6%), compared to 56.0% (95% CI 43.9%-64.9%) for LF-LAM.
32357197	11	120	theme	%	2189:2189	arg1	%					2184:2184	87.1%	2180:2184	87.1% (95% CI 79.3%-93.6%)	2180:2205	In patients with CD4 count ≤ 100 cells/μl, SILVAMP-LAM sensitivity was 87.1% (95% CI 79.3%-93.6%), compared to 56.0% (95% CI 43.9%-64.9%) for LF-LAM.
32357197	6	121	theme	symptom	1283:1289	arg1	screen					1291:1296	symptom screen	1283:1296	a positive WHO symptom screen for TB	1268:1303	Most participants (94%) had a positive WHO symptom screen for TB on enrollment, and 45% were diagnosed with microbiologically confirmed TB, using mycobacterial culture or Xpert MTB/RIF testing of sputum, urine, or blood.
32357197	3	122	theme	individual	715:724	arg1	meta-analysis					743:755	an individual participant data meta-analysis	712:755	an individual participant data meta-analysis of the diagnostic accuracy of SILVAMP-LAM in adult PLHIV	712:812	Here, we present an individual participant data meta-analysis of the diagnostic accuracy of SILVAMP-LAM in adult PLHIV, including both published and unpublished data.
32357197	2	123	theme	earlier	623:629	arg1	assay					635:639	an earlier LAM assay	620:639	an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM])	620:692	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	0	124	theme	assay	82:86	arg1	accuracy					11:18	Diagnostic accuracy	0:18	Diagnostic accuracy of a novel tuberculosis point-of-care urine lipoarabinomannan assay for people living with HIV: A meta-analysis of individual in- and outpatient data.	0:169	Diagnostic accuracy of a novel tuberculosis point-of-care urine lipoarabinomannan assay for people living with HIV: A meta-analysis of individual in- and outpatient data.
32357197	6	125	theme	positive	1270:1277	arg1	screen					1291:1296	symptom screen	1283:1296	a positive WHO symptom screen for TB	1268:1303	Most participants (94%) had a positive WHO symptom screen for TB on enrollment, and 45% were diagnosed with microbiologically confirmed TB, using mycobacterial culture or Xpert MTB/RIF testing of sputum, urine, or blood.
32357197	6	126	theme	urine	1444:1448	arg1	testing					1425:1431	Xpert MTB/RIF testing	1411:1431	Xpert MTB/RIF testing of sputum, urine, or blood	1411:1458	Most participants (94%) had a positive WHO symptom screen for TB on enrollment, and 45% were diagnosed with microbiologically confirmed TB, using mycobacterial culture or Xpert MTB/RIF testing of sputum, urine, or blood.
32357197	6	126	theme	urine	1444:1448	arg1	culture					1400:1406	mycobacterial culture	1386:1406	mycobacterial culture	1386:1406	Most participants (94%) had a positive WHO symptom screen for TB on enrollment, and 45% were diagnosed with microbiologically confirmed TB, using mycobacterial culture or Xpert MTB/RIF testing of sputum, urine, or blood.
32357197	13	127	theme	CI	2490:2491	arg1	%					2483:2483	43.9%	2479:2483	43.9% (95% CI 34.3%-53.9%)	2479:2504	In those with CD4 count > 200 cells/μl, SILVAMP-LAM sensitivity was 43.9% (95% CI 34.3%-53.9%), compared to 10.9% (95% CI 5.2%-18.4%) for LF-LAM.
32357197	13	127	theme	CI	2490:2491	arg1	%					2503:2503	95% CI 34.3%-53.9%	2486:2503	95% CI 34.3%-53.9%	2486:2503	In those with CD4 count > 200 cells/μl, SILVAMP-LAM sensitivity was 43.9% (95% CI 34.3%-53.9%), compared to 10.9% (95% CI 5.2%-18.4%) for LF-LAM.
32357197	10	128	theme	%	2099:2099	arg1	%					2105:2105	95% CI 19.1%-43.7%	2088:2105	95% CI 19.1%-43.7%	2088:2105	Using a composite reference standard (which included patients with both microbiologically confirmed as well as clinically diagnosed TB), SILVAMP-LAM sensitivity was 65.8% (95% CI 55.9%-74.6%), and that of LF-LAM 31.4% (95% CI 19.1%-43.7%).
32357197	10	128	theme	%	2099:2099	arg1	%					2085:2085	LF-LAM 31.4%	2074:2085	LF-LAM 31.4% (95% CI 19.1%-43.7%)	2074:2106	Using a composite reference standard (which included patients with both microbiologically confirmed as well as clinically diagnosed TB), SILVAMP-LAM sensitivity was 65.8% (95% CI 55.9%-74.6%), and that of LF-LAM 31.4% (95% CI 19.1%-43.7%).
32357197	15	129	theme	biobanked	2756:2764	arg1	use					2749:2751	the use	2745:2751	the use of biobanked, rather than fresh urine samples, and testing	2745:2810	Limitations of this study include the use of biobanked, rather than fresh urine samples, and testing by skilled laboratory technicians in research laboratories, rather than at the point of care.
32357197	2	130	theme	novel	419:423	arg1	LAM					472:474	Fujifilm SILVAMP TB LAM	452:474	Fujifilm SILVAMP TB LAM (SILVAMP-LAM)	452:488	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	2	130	theme	novel	419:423	arg1	assay					445:449	A novel, rapid lateral flow assay	417:449	A novel, rapid lateral flow assay	417:449	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	2	130	theme	novel	419:423	arg1	sensitive					578:586	sensitive	578:586	sensitive	578:586	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	6	131	theme	sputum	1436:1441	arg1	testing					1425:1431	Xpert MTB/RIF testing	1411:1431	Xpert MTB/RIF testing of sputum, urine, or blood	1411:1458	Most participants (94%) had a positive WHO symptom screen for TB on enrollment, and 45% were diagnosed with microbiologically confirmed TB, using mycobacterial culture or Xpert MTB/RIF testing of sputum, urine, or blood.
32357197	6	131	theme	sputum	1436:1441	arg1	culture					1400:1406	mycobacterial culture	1386:1406	mycobacterial culture	1386:1406	Most participants (94%) had a positive WHO symptom screen for TB on enrollment, and 45% were diagnosed with microbiologically confirmed TB, using mycobacterial culture or Xpert MTB/RIF testing of sputum, urine, or blood.
32357197	10	132	theme	CI	2092:2093	arg1	%					2105:2105	95% CI 19.1%-43.7%	2088:2105	95% CI 19.1%-43.7%	2088:2105	Using a composite reference standard (which included patients with both microbiologically confirmed as well as clinically diagnosed TB), SILVAMP-LAM sensitivity was 65.8% (95% CI 55.9%-74.6%), and that of LF-LAM 31.4% (95% CI 19.1%-43.7%).
32357197	10	132	theme	CI	2092:2093	arg1	%					2085:2085	LF-LAM 31.4%	2074:2085	LF-LAM 31.4% (95% CI 19.1%-43.7%)	2074:2106	Using a composite reference standard (which included patients with both microbiologically confirmed as well as clinically diagnosed TB), SILVAMP-LAM sensitivity was 65.8% (95% CI 55.9%-74.6%), and that of LF-LAM 31.4% (95% CI 19.1%-43.7%).
32357197	5	133	theme	eligibility	1062:1072	arg1	criteria					1074:1081	eligibility criteria	1062:1081	eligibility criteria	1062:1081	Of the 1,595 PLHIV who met eligibility criteria, the majority (61%) were inpatients, median age was 37 years (IQR 30-43), 43% had a CD4 count ≤ 100 cells/μl, and 35% were receiving antiretroviral therapy.
32357197	15	134	theme	study	2731:2735	arg1	Limitations					2711:2721	Limitations	2711:2721	Limitations of this study	2711:2735	Limitations of this study include the use of biobanked, rather than fresh urine samples, and testing by skilled laboratory technicians in research laboratories, rather than at the point of care.
32357197	3	135	theme	data	738:741	arg1	meta-analysis					743:755	an individual participant data meta-analysis	712:755	an individual participant data meta-analysis of the diagnostic accuracy of SILVAMP-LAM in adult PLHIV	712:812	Here, we present an individual participant data meta-analysis of the diagnostic accuracy of SILVAMP-LAM in adult PLHIV, including both published and unpublished data.
32357197	5	136	theme	antiretroviral	1216:1229	arg1	therapy					1231:1237	antiretroviral therapy	1216:1237	antiretroviral therapy	1216:1237	Of the 1,595 PLHIV who met eligibility criteria, the majority (61%) were inpatients, median age was 37 years (IQR 30-43), 43% had a CD4 count ≤ 100 cells/μl, and 35% were receiving antiretroviral therapy.
32357197	14	137	theme	CI	2694:2695	arg1	%					2687:2687	95.3%	2683:2687	95.3% (95% CI 92.2%-97.7%)	2683:2708	Using a microbiological reference standard, the specificity of SILVAMP-LAM was 90.9% (95% CI 87.2%-93.7%), and that of LF-LAM 95.3% (95% CI 92.2%-97.7%).
32357197	14	137	theme	CI	2694:2695	arg1	%					2707:2707	95% CI 92.2%-97.7%	2690:2707	95% CI 92.2%-97.7%	2690:2707	Using a microbiological reference standard, the specificity of SILVAMP-LAM was 90.9% (95% CI 87.2%-93.7%), and that of LF-LAM 95.3% (95% CI 92.2%-97.7%).
32357197	7	138	from	inpatients	1492:1501	arg1	data					1482:1485	Previously published data	1461:1485	Previously published data from inpatients	1461:1501	Previously published data from inpatients were combined with unpublished data from outpatients.
32357197	2	139	theme	flow	440:443	arg1	LAM					472:474	Fujifilm SILVAMP TB LAM	452:474	Fujifilm SILVAMP TB LAM (SILVAMP-LAM)	452:488	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	2	139	theme	flow	440:443	arg1	assay					445:449	A novel, rapid lateral flow assay	417:449	A novel, rapid lateral flow assay	417:449	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	2	139	theme	flow	440:443	arg1	sensitive					578:586	sensitive	578:586	sensitive	578:586	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	6	140	theme	Xpert	1411:1415	arg1	testing					1425:1431	Xpert MTB/RIF testing	1411:1431	Xpert MTB/RIF testing of sputum, urine, or blood	1411:1458	Most participants (94%) had a positive WHO symptom screen for TB on enrollment, and 45% were diagnosed with microbiologically confirmed TB, using mycobacterial culture or Xpert MTB/RIF testing of sputum, urine, or blood.
32357197	9	141	theme	overall	1734:1740	arg1	sensitivity					1742:1752	the overall sensitivity	1730:1752	the overall sensitivity for TB detection	1730:1769	Applying a microbiological reference standard for assessment of sensitivity, the overall sensitivity for TB detection was 70.7% (95% CI 59.0%-80.8%) for SILVAMP-LAM compared to 34.9% (95% CI 19.5%-50.9%) for LF-LAM.
32357197	9	141	theme	overall	1734:1740	arg1	%					1779:1779	70.7%	1775:1779	70.7% (95% CI 59.0%-80.8%) for SILVAMP-LAM	1775:1816	Applying a microbiological reference standard for assessment of sensitivity, the overall sensitivity for TB detection was 70.7% (95% CI 59.0%-80.8%) for SILVAMP-LAM compared to 34.9% (95% CI 19.5%-50.9%) for LF-LAM.
32357197	15	142	theme	laboratory	2823:2832	arg1	technicians					2834:2844	skilled laboratory technicians	2815:2844	skilled laboratory technicians in research laboratories	2815:2869	Limitations of this study include the use of biobanked, rather than fresh urine samples, and testing by skilled laboratory technicians in research laboratories, rather than at the point of care.
32357197	5	143	theme	median	1120:1125	arg1	age					1127:1129	median age	1120:1129	median age	1120:1129	Of the 1,595 PLHIV who met eligibility criteria, the majority (61%) were inpatients, median age was 37 years (IQR 30-43), 43% had a CD4 count ≤ 100 cells/μl, and 35% were receiving antiretroviral therapy.
32357197	3	144	theme	diagnostic	764:773	arg1	accuracy					775:782	the diagnostic accuracy	760:782	the diagnostic accuracy of SILVAMP-LAM	760:797	Here, we present an individual participant data meta-analysis of the diagnostic accuracy of SILVAMP-LAM in adult PLHIV, including both published and unpublished data.
32357197	2	145	from	presence	503:510	arg1	urine					545:549	urine	545:549	urine	545:549	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	14	146	theme	standard	2591:2598	arg1	reference					2581:2589	a microbiological reference standard	2563:2598	a microbiological reference standard	2563:2598	Using a microbiological reference standard, the specificity of SILVAMP-LAM was 90.9% (95% CI 87.2%-93.7%), and that of LF-LAM 95.3% (95% CI 92.2%-97.7%).
32357197	15	147	theme	research	2849:2856	arg1	laboratories					2858:2869	research laboratories	2849:2869	research laboratories	2849:2869	Limitations of this study include the use of biobanked, rather than fresh urine samples, and testing by skilled laboratory technicians in research laboratories, rather than at the point of care.
32357197	15	148	theme	urine	2785:2789	arg1	samples					2791:2797	fresh urine samples	2779:2797	fresh urine samples	2779:2797	Limitations of this study include the use of biobanked, rather than fresh urine samples, and testing by skilled laboratory technicians in research laboratories, rather than at the point of care.
32357197	5	149	contain	had	1161:1163	arg2	count					1171:1175	a CD4 count	1165:1175	a CD4 count	1165:1175	Of the 1,595 PLHIV who met eligibility criteria, the majority (61%) were inpatients, median age was 37 years (IQR 30-43), 43% had a CD4 count ≤ 100 cells/μl, and 35% were receiving antiretroviral therapy.
32357197	5	149	contain	had	1161:1163	arg1	%					1159:1159	43%	1157:1159	43%	1157:1159	Of the 1,595 PLHIV who met eligibility criteria, the majority (61%) were inpatients, median age was 37 years (IQR 30-43), 43% had a CD4 count ≤ 100 cells/μl, and 35% were receiving antiretroviral therapy.
32357197	0	150	theme	tuberculosis	31:42	arg1	assay					82:86	a novel tuberculosis point-of-care urine lipoarabinomannan assay	23:86	a novel tuberculosis point-of-care urine lipoarabinomannan assay	23:86	Diagnostic accuracy of a novel tuberculosis point-of-care urine lipoarabinomannan assay for people living with HIV: A meta-analysis of individual in- and outpatient data.
32357197	2	151	theme	flow	673:676	arg1	[LF-LAM					684:690	lateral flow assay [LF-LAM	665:690	Alere Determine TB LAM lateral flow assay [LF-LAM	642:690	A novel, rapid lateral flow assay, Fujifilm SILVAMP TB LAM (SILVAMP-LAM), detects the presence of TB lipoarabinomannan (LAM) in urine, and is substantially more sensitive for diagnosing TB in PLHIV than an earlier LAM assay (Alere Determine TB LAM lateral flow assay [LF-LAM]).
32357197	10	152	theme	%	2052:2052	arg1	%					2058:2058	95% CI 55.9%-74.6%	2041:2058	95% CI 55.9%-74.6%	2041:2058	Using a composite reference standard (which included patients with both microbiologically confirmed as well as clinically diagnosed TB), SILVAMP-LAM sensitivity was 65.8% (95% CI 55.9%-74.6%), and that of LF-LAM 31.4% (95% CI 19.1%-43.7%).
32357197	10	152	theme	%	2052:2052	arg1	%					2038:2038	65.8%	2034:2038	65.8% (95% CI 55.9%-74.6%)	2034:2059	Using a composite reference standard (which included patients with both microbiologically confirmed as well as clinically diagnosed TB), SILVAMP-LAM sensitivity was 65.8% (95% CI 55.9%-74.6%), and that of LF-LAM 31.4% (95% CI 19.1%-43.7%).
32357197	17	153	theme	CD4	3101:3103	arg1	count					3105:3109	CD4 count	3101:3109	CD4 count ≤ 100 cells/μl	3101:3124	The sensitivity of SILVAMP-LAM was highest in patients with CD4 count ≤ 100 cells/μl.
32357197	11	154	with	patients	2112:2119	arg1	count					2130:2134	CD4 count	2126:2134	CD4 count ≤ 100 cells/μl	2126:2149	In patients with CD4 count ≤ 100 cells/μl, SILVAMP-LAM sensitivity was 87.1% (95% CI 79.3%-93.6%), compared to 56.0% (95% CI 43.9%-64.9%) for LF-LAM.
32357197	10	155	theme	SILVAMP-LAM	2006:2016	arg1	sensitivity					2018:2028	SILVAMP-LAM sensitivity	2006:2028	SILVAMP-LAM sensitivity	2006:2028	Using a composite reference standard (which included patients with both microbiologically confirmed as well as clinically diagnosed TB), SILVAMP-LAM sensitivity was 65.8% (95% CI 55.9%-74.6%), and that of LF-LAM 31.4% (95% CI 19.1%-43.7%).
32357197	0	156	theme	urine	58:62	arg1	assay					82:86	a novel tuberculosis point-of-care urine lipoarabinomannan assay	23:86	a novel tuberculosis point-of-care urine lipoarabinomannan assay	23:86	Diagnostic accuracy of a novel tuberculosis point-of-care urine lipoarabinomannan assay for people living with HIV: A meta-analysis of individual in- and outpatient data.
32357197	13	157	theme	%	2528:2528	arg1	%					2542:2542	95% CI 5.2%-18.4%	2526:2542	95% CI 5.2%-18.4%	2526:2542	In those with CD4 count > 200 cells/μl, SILVAMP-LAM sensitivity was 43.9% (95% CI 34.3%-53.9%), compared to 10.9% (95% CI 5.2%-18.4%) for LF-LAM.
32357197	13	157	theme	%	2528:2528	arg1	%					2523:2523	10.9%	2519:2523	10.9% (95% CI 5.2%-18.4%) for LF-LAM	2519:2554	In those with CD4 count > 200 cells/μl, SILVAMP-LAM sensitivity was 43.9% (95% CI 34.3%-53.9%), compared to 10.9% (95% CI 5.2%-18.4%) for LF-LAM.
32357197	12	158	with	patients	2262:2269	arg1	cells/μl					2294:2301	CD4 count 101-200 cells/μl	2276:2301	CD4 count 101-200 cells/μl	2276:2301	In patients with CD4 count 101-200 cells/μl, SILVAMP-LAM sensitivity was 62.7% (95% CI 52.4%-71.9%), compared to 25.3% (95% CI 15.8%-34.9%) for LF-LAM.
32357197	3	159	theme	published	830:838	arg1	data					856:859	both published and unpublished data	825:859	both published and unpublished data	825:859	Here, we present an individual participant data meta-analysis of the diagnostic accuracy of SILVAMP-LAM in adult PLHIV, including both published and unpublished data.
32357197	10	160	theme	CI	2045:2046	arg1	%					2058:2058	95% CI 55.9%-74.6%	2041:2058	95% CI 55.9%-74.6%	2041:2058	Using a composite reference standard (which included patients with both microbiologically confirmed as well as clinically diagnosed TB), SILVAMP-LAM sensitivity was 65.8% (95% CI 55.9%-74.6%), and that of LF-LAM 31.4% (95% CI 19.1%-43.7%).
32357197	10	160	theme	CI	2045:2046	arg1	%					2038:2038	65.8%	2034:2038	65.8% (95% CI 55.9%-74.6%)	2034:2059	Using a composite reference standard (which included patients with both microbiologically confirmed as well as clinically diagnosed TB), SILVAMP-LAM sensitivity was 65.8% (95% CI 55.9%-74.6%), and that of LF-LAM 31.4% (95% CI 19.1%-43.7%).
32357197	11	161	theme	SILVAMP-LAM	2152:2162	arg1	sensitivity					2164:2174	SILVAMP-LAM sensitivity	2152:2174	SILVAMP-LAM sensitivity	2152:2174	In patients with CD4 count ≤ 100 cells/μl, SILVAMP-LAM sensitivity was 87.1% (95% CI 79.3%-93.6%), compared to 56.0% (95% CI 43.9%-64.9%) for LF-LAM.
32357197	11	162	theme	%	2229:2229	arg1	%					2224:2224	56.0%	2220:2224	56.0% (95% CI 43.9%-64.9%) for LF-LAM	2220:2256	In patients with CD4 count ≤ 100 cells/μl, SILVAMP-LAM sensitivity was 87.1% (95% CI 79.3%-93.6%), compared to 56.0% (95% CI 43.9%-64.9%) for LF-LAM.
32357197	11	162	theme	%	2229:2229	arg1	%					2244:2244	95% CI 43.9%-64.9%	2227:2244	95% CI 43.9%-64.9%	2227:2244	In patients with CD4 count ≤ 100 cells/μl, SILVAMP-LAM sensitivity was 87.1% (95% CI 79.3%-93.6%), compared to 56.0% (95% CI 43.9%-64.9%) for LF-LAM.
32357197	9	163	theme	%	1784:1784	arg1	%					1779:1779	70.7%	1775:1779	70.7% (95% CI 59.0%-80.8%) for SILVAMP-LAM	1775:1816	Applying a microbiological reference standard for assessment of sensitivity, the overall sensitivity for TB detection was 70.7% (95% CI 59.0%-80.8%) for SILVAMP-LAM compared to 34.9% (95% CI 19.5%-50.9%) for LF-LAM.
32357197	9	163	theme	%	1784:1784	arg1	%					1799:1799	95% CI 59.0%-80.8%	1782:1799	95% CI 59.0%-80.8%	1782:1799	Applying a microbiological reference standard for assessment of sensitivity, the overall sensitivity for TB detection was 70.7% (95% CI 59.0%-80.8%) for SILVAMP-LAM compared to 34.9% (95% CI 19.5%-50.9%) for LF-LAM.
32357197	1	164	theme	common	212:217	arg1	cause					219:223	the most common cause	203:223	the most common cause of death in people living with HIV (PLHIV)	203:266	BACKGROUND Tuberculosis (TB) is the most common cause of death in people living with HIV (PLHIV), yet TB often goes undiagnosed since many patients are not able to produce a sputum specimen, and traditional diagnostics are costly or unavailable.
32357197	1	164	theme	common	212:217	arg1	Tuberculosis					182:193	BACKGROUND Tuberculosis	171:193	BACKGROUND Tuberculosis (TB)	171:198	BACKGROUND Tuberculosis (TB) is the most common cause of death in people living with HIV (PLHIV), yet TB often goes undiagnosed since many patients are not able to produce a sputum specimen, and traditional diagnostics are costly or unavailable.
32357197	0	165	dep	accuracy	11:18	arg1	meta-analysis					118:130	A meta-analysis	116:130	A meta-analysis of individual in-	116:148	Diagnostic accuracy of a novel tuberculosis point-of-care urine lipoarabinomannan assay for people living with HIV: A meta-analysis of individual in- and outpatient data.
32357197	0	165	dep	accuracy	11:18	arg1	data					165:168	outpatient data	154:168	outpatient data	154:168	Diagnostic accuracy of a novel tuberculosis point-of-care urine lipoarabinomannan assay for people living with HIV: A meta-analysis of individual in- and outpatient data.
32357197	4	166	theme	cohort	938:943	arg1	studies					945:951	5 prospective cohort studies	924:951	5 prospective cohort studies in South Africa (3 cohorts), Vietnam, and Ghana, carried out during 2012 to 2017	924:1032	METHODS AND FINDINGS Adult PLHIV (≥18 years) were assessed in 5 prospective cohort studies in South Africa (3 cohorts), Vietnam, and Ghana, carried out during 2012 to 2017.
32357197	17	167	with	patients	3087:3094	arg1	count					3105:3109	CD4 count	3101:3109	CD4 count ≤ 100 cells/μl	3101:3124	The sensitivity of SILVAMP-LAM was highest in patients with CD4 count ≤ 100 cells/μl.
32357197	5	168	theme	PLHIV	1048:1052	arg1	majority					1088:1095	the majority	1084:1095	the majority (61%)	1084:1101	Of the 1,595 PLHIV who met eligibility criteria, the majority (61%) were inpatients, median age was 37 years (IQR 30-43), 43% had a CD4 count ≤ 100 cells/μl, and 35% were receiving antiretroviral therapy.
32357197	5	168	theme	PLHIV	1048:1052	arg1	inpatients					1108:1117	inpatients	1108:1117	inpatients	1108:1117	Of the 1,595 PLHIV who met eligibility criteria, the majority (61%) were inpatients, median age was 37 years (IQR 30-43), 43% had a CD4 count ≤ 100 cells/μl, and 35% were receiving antiretroviral therapy.
32499514	11	0	with	neutralization	1870:1883	arg1	activation					1950:1959	dose-dependent, inhibiting, LPS-mediated Toll-like receptor activation	1890:1959	activation	1950:1959	Moreover, PA-13 showed an anti-inflammatory response via lipopolysaccharide (LPS) neutralization with dose-dependent, inhibiting, LPS-mediated Toll-like receptor activation.
32499514	3	1	theme	key	527:529	arg1	parameters					562:571	key structural and physicochemical parameters	527:571	key structural and physicochemical parameters	527:571	In this study, we utilized key structural and physicochemical parameters, in combination with rational engineering, to design novel short α-helical hybrid peptides inspired by the well-known natural peptides, cathelicidin and aurein.
32499514	8	2	theme	shorter	1338:1344	arg1	derivatives					1346:1356	shorter derivatives	1338:1356	shorter derivatives	1338:1356	Strikingly, C-terminal truncation of hybrid peptides containing only the α-helical segment (PA-18) and shorter derivatives confer potent antimicrobial activity with reduced hemolytic activity in a length-dependent manner.
32499514	10	3	theme	membrane	1742:1749	arg1	depolarization					1751:1764	membrane depolarization	1742:1764	membrane depolarization	1742:1764	PA-13 maintained antimicrobial activity in the presence of physiological salts and displayed rapid binding and penetration activity which resulted in membrane depolarization and permeabilization.
32499514	7	4	theme	higher	1135:1140	arg1	activity					1156:1163	higher antibacterial activity	1135:1163	higher antibacterial activity	1135:1163	The results demonstrated that P7A3 had higher antibacterial activity than the parental peptides with unexpectedly high hemolytic activity.
32499514	8	5	theme	C-terminal	1247:1256	arg1	truncation					1258:1267	C-terminal truncation	1247:1267	C-terminal truncation of hybrid peptides containing only the α-helical segment (PA-18)	1247:1332	Strikingly, C-terminal truncation of hybrid peptides containing only the α-helical segment (PA-18) and shorter derivatives confer potent antimicrobial activity with reduced hemolytic activity in a length-dependent manner.
32499514	3	6	theme	structural	531:540	arg1	parameters					562:571	key structural and physicochemical parameters	527:571	key structural and physicochemical parameters	527:571	In this study, we utilized key structural and physicochemical parameters, in combination with rational engineering, to design novel short α-helical hybrid peptides inspired by the well-known natural peptides, cathelicidin and aurein.
32499514	8	7	from	activity	1418:1425	arg1	manner					1449:1454	a length-dependent manner	1430:1454	a length-dependent manner	1430:1454	Strikingly, C-terminal truncation of hybrid peptides containing only the α-helical segment (PA-18) and shorter derivatives confer potent antimicrobial activity with reduced hemolytic activity in a length-dependent manner.
32499514	12	8	theme	new	2091:2093	arg1	agents					2109:2114	new antibacterial agents	2091:2114	new antibacterial agents	2091:2114	This study revealed the therapeutic potency of a novel hybrid peptide, and supports the use of rational design in development of new antibacterial agents.
32499514	10	9	theme	salts	1665:1669	arg1	presence					1639:1646	the presence	1635:1646	the presence of physiological salts	1635:1669	PA-13 maintained antimicrobial activity in the presence of physiological salts and displayed rapid binding and penetration activity which resulted in membrane depolarization and permeabilization.
32499514	12	10	theme	rational	2057:2064	arg1	design					2066:2071	rational design	2057:2071	rational design	2057:2071	This study revealed the therapeutic potency of a novel hybrid peptide, and supports the use of rational design in development of new antibacterial agents.
32499514	4	11	theme	sequence	812:819	arg1	templates					821:829	sequence templates	812:829	sequence templates of cathelicidin (P0) and aurein (A0)	812:866	By comparing homologous sequences and abstracting the conserved residue type, sequence templates of cathelicidin (P0) and aurein (A0) were obtained.
32499514	3	12	theme	rational	594:601	arg1	engineering					603:613	rational engineering	594:613	rational engineering	594:613	In this study, we utilized key structural and physicochemical parameters, in combination with rational engineering, to design novel short α-helical hybrid peptides inspired by the well-known natural peptides, cathelicidin and aurein.
32499514	1	13	theme	drug-resistant	268:281	arg1	infections					283:292	drug-resistant infections	268:292	drug-resistant infections	268:292	Antimicrobial peptides (AMPs) are promising alternatives to classical antibiotics for the treatment of drug-resistant infections.
32499514	4	14	theme	conserved	788:796	arg1	type					806:809	the conserved residue type	784:809	the conserved residue type	784:809	By comparing homologous sequences and abstracting the conserved residue type, sequence templates of cathelicidin (P0) and aurein (A0) were obtained.
32499514	8	15	theme	antimicrobial	1372:1384	arg1	activity					1386:1393	potent antimicrobial activity	1365:1393	potent antimicrobial activity	1365:1393	Strikingly, C-terminal truncation of hybrid peptides containing only the α-helical segment (PA-18) and shorter derivatives confer potent antimicrobial activity with reduced hemolytic activity in a length-dependent manner.
32499514	5	16	theme	residue	976:982	arg1	composition					984:994	their residue composition	970:994	their residue composition	970:994	Two peptide derivatives, P7 and A3, were generated by amino acid substitution based on their residue composition and distribution.
32499514	1	17	theme	infections	283:292	arg1	treatment					255:263	the treatment	251:263	the treatment of drug-resistant infections	251:292	Antimicrobial peptides (AMPs) are promising alternatives to classical antibiotics for the treatment of drug-resistant infections.
32499514	7	18	theme	high	1210:1213	arg1	activity					1225:1232	unexpectedly high hemolytic activity	1197:1232	unexpectedly high hemolytic activity	1197:1232	The results demonstrated that P7A3 had higher antibacterial activity than the parental peptides with unexpectedly high hemolytic activity.
32499514	2	19	theme	biological	442:451	arg1	parameters					453:462	desired biological parameters	434:462	desired biological parameters	434:462	Due to their versatility and unlimited sequence space, AMPs can be rationally designed by modulating physicochemical determinants to favor desired biological parameters and turned into novel therapeutics.
32499514	8	20	theme	reduced	1400:1406	arg1	activity					1418:1425	reduced hemolytic activity	1400:1425	reduced hemolytic activity in a length-dependent manner	1400:1454	Strikingly, C-terminal truncation of hybrid peptides containing only the α-helical segment (PA-18) and shorter derivatives confer potent antimicrobial activity with reduced hemolytic activity in a length-dependent manner.
32499514	6	21	theme	P7A3	1077:1080	arg1	analog					1067:1072	a hybrid analog	1058:1072	a hybrid analog of P7A3	1058:1080	In order to enhance antimicrobial activity, a hybrid analog of P7A3 was designed.
32499514	6	22	theme	hybrid	1060:1065	arg1	analog					1067:1072	a hybrid analog	1058:1072	a hybrid analog of P7A3	1058:1080	In order to enhance antimicrobial activity, a hybrid analog of P7A3 was designed.
32499514	7	23	theme	hemolytic	1215:1223	arg1	activity					1225:1232	unexpectedly high hemolytic activity	1197:1232	unexpectedly high hemolytic activity	1197:1232	The results demonstrated that P7A3 had higher antibacterial activity than the parental peptides with unexpectedly high hemolytic activity.
32499514	8	24	contain	containing	1288:1297	arg1	peptides					1279:1286	hybrid peptides	1272:1286	hybrid peptides containing only the α-helical segment (PA-18)	1272:1332	Strikingly, C-terminal truncation of hybrid peptides containing only the α-helical segment (PA-18) and shorter derivatives confer potent antimicrobial activity with reduced hemolytic activity in a length-dependent manner.
32499514	8	24	contain	containing	1288:1297	arg2	segment					1318:1324	only the α-helical segment	1299:1324	only the α-helical segment (PA-18)	1299:1332	Strikingly, C-terminal truncation of hybrid peptides containing only the α-helical segment (PA-18) and shorter derivatives confer potent antimicrobial activity with reduced hemolytic activity in a length-dependent manner.
32499514	8	24	contain	containing	1288:1297	arg2	PA-18					1327:1331	PA-18	1327:1331	PA-18	1327:1331	Strikingly, C-terminal truncation of hybrid peptides containing only the α-helical segment (PA-18) and shorter derivatives confer potent antimicrobial activity with reduced hemolytic activity in a length-dependent manner.
32499514	5	25	theme	acid	943:946	arg1	substitution					948:959	amino acid substitution	937:959	amino acid substitution based on their residue composition and distribution	937:1011	Two peptide derivatives, P7 and A3, were generated by amino acid substitution based on their residue composition and distribution.
32499514	5	26	theme	peptide	887:893	arg1	A3					915:916	A3	915:916	A3	915:916	Two peptide derivatives, P7 and A3, were generated by amino acid substitution based on their residue composition and distribution.
32499514	5	26	theme	peptide	887:893	arg1	P7					908:909	P7	908:909	P7	908:909	Two peptide derivatives, P7 and A3, were generated by amino acid substitution based on their residue composition and distribution.
32499514	5	26	theme	peptide	887:893	arg1	derivatives					895:905	Two peptide derivatives	883:905	Two peptide derivatives	883:905	Two peptide derivatives, P7 and A3, were generated by amino acid substitution based on their residue composition and distribution.
32499514	2	27	theme	novel	480:484	arg1	therapeutics					486:497	novel therapeutics	480:497	novel therapeutics	480:497	Due to their versatility and unlimited sequence space, AMPs can be rationally designed by modulating physicochemical determinants to favor desired biological parameters and turned into novel therapeutics.
32499514	10	28	theme	antimicrobial	1609:1621	arg1	activity					1623:1630	antimicrobial activity	1609:1630	antimicrobial activity	1609:1630	PA-13 maintained antimicrobial activity in the presence of physiological salts and displayed rapid binding and penetration activity which resulted in membrane depolarization and permeabilization.
32499514	12	29	theme	agents	2109:2114	arg1	development					2076:2086	development	2076:2086	development of new antibacterial agents	2076:2114	This study revealed the therapeutic potency of a novel hybrid peptide, and supports the use of rational design in development of new antibacterial agents.
32499514	2	30	theme	sequence	334:341	arg1	space					343:347	unlimited sequence space	324:347	unlimited sequence space	324:347	Due to their versatility and unlimited sequence space, AMPs can be rationally designed by modulating physicochemical determinants to favor desired biological parameters and turned into novel therapeutics.
32499514	2	31	theme	physicochemical	396:410	arg1	determinants					412:423	physicochemical determinants	396:423	physicochemical determinants	396:423	Due to their versatility and unlimited sequence space, AMPs can be rationally designed by modulating physicochemical determinants to favor desired biological parameters and turned into novel therapeutics.
32499514	10	32	theme	rapid	1685:1689	arg1	binding					1691:1697	rapid binding	1685:1697	rapid binding	1685:1697	PA-13 maintained antimicrobial activity in the presence of physiological salts and displayed rapid binding and penetration activity which resulted in membrane depolarization and permeabilization.
32499514	11	33	theme	Toll-like	1931:1939	arg1	activation					1950:1959	dose-dependent, inhibiting, LPS-mediated Toll-like receptor activation	1890:1959	activation	1950:1959	Moreover, PA-13 showed an anti-inflammatory response via lipopolysaccharide (LPS) neutralization with dose-dependent, inhibiting, LPS-mediated Toll-like receptor activation.
32499514	12	34	theme	peptide	2024:2030	arg1	potency					1998:2004	the therapeutic potency	1982:2004	the therapeutic potency of a novel hybrid peptide	1982:2030	This study revealed the therapeutic potency of a novel hybrid peptide, and supports the use of rational design in development of new antibacterial agents.
32499514	2	35	theme	unlimited	324:332	arg1	space					343:347	unlimited sequence space	324:347	unlimited sequence space	324:347	Due to their versatility and unlimited sequence space, AMPs can be rationally designed by modulating physicochemical determinants to favor desired biological parameters and turned into novel therapeutics.
32499514	8	36	theme	peptides	1279:1286	arg1	derivatives					1346:1356	shorter derivatives	1338:1356	shorter derivatives	1338:1356	Strikingly, C-terminal truncation of hybrid peptides containing only the α-helical segment (PA-18) and shorter derivatives confer potent antimicrobial activity with reduced hemolytic activity in a length-dependent manner.
32499514	8	36	theme	peptides	1279:1286	arg1	truncation					1258:1267	C-terminal truncation	1247:1267	C-terminal truncation of hybrid peptides containing only the α-helical segment (PA-18)	1247:1332	Strikingly, C-terminal truncation of hybrid peptides containing only the α-helical segment (PA-18) and shorter derivatives confer potent antimicrobial activity with reduced hemolytic activity in a length-dependent manner.
32499514	7	37	theme	parental	1174:1181	arg1	peptides					1183:1190	the parental peptides	1170:1190	the parental peptides	1170:1190	The results demonstrated that P7A3 had higher antibacterial activity than the parental peptides with unexpectedly high hemolytic activity.
32499514	3	38	theme	α-helical	638:646	arg1	cathelicidin					709:720	cathelicidin	709:720	cathelicidin	709:720	In this study, we utilized key structural and physicochemical parameters, in combination with rational engineering, to design novel short α-helical hybrid peptides inspired by the well-known natural peptides, cathelicidin and aurein.
32499514	3	38	theme	α-helical	638:646	arg1	aurein					726:731	aurein	726:731	aurein	726:731	In this study, we utilized key structural and physicochemical parameters, in combination with rational engineering, to design novel short α-helical hybrid peptides inspired by the well-known natural peptides, cathelicidin and aurein.
32499514	3	38	theme	α-helical	638:646	arg1	peptides					655:662	novel short α-helical hybrid peptides	626:662	novel short α-helical hybrid peptides inspired by the well-known natural peptides	626:706	In this study, we utilized key structural and physicochemical parameters, in combination with rational engineering, to design novel short α-helical hybrid peptides inspired by the well-known natural peptides, cathelicidin and aurein.
32499514	0	39	theme	membrane-active	106:120	arg1	mechanisms					122:131	membrane-active mechanisms	106:131	membrane-active mechanisms against Pseudomonas aeruginosa	106:162	A novel, rationally designed, hybrid antimicrobial peptide, inspired by cathelicidin and aurein, exhibits membrane-active mechanisms against Pseudomonas aeruginosa.
32499514	5	40	theme	amino	937:941	arg1	substitution					948:959	amino acid substitution	937:959	amino acid substitution based on their residue composition and distribution	937:1011	Two peptide derivatives, P7 and A3, were generated by amino acid substitution based on their residue composition and distribution.
32499514	4	41	theme	aurein	856:861	arg1	templates					821:829	sequence templates	812:829	sequence templates of cathelicidin (P0) and aurein (A0)	812:866	By comparing homologous sequences and abstracting the conserved residue type, sequence templates of cathelicidin (P0) and aurein (A0) were obtained.
32499514	12	42	theme	novel	2011:2015	arg1	peptide					2024:2030	a novel hybrid peptide	2009:2030	a novel hybrid peptide	2009:2030	This study revealed the therapeutic potency of a novel hybrid peptide, and supports the use of rational design in development of new antibacterial agents.
32499514	8	43	theme	hemolytic	1408:1416	arg1	activity					1418:1425	reduced hemolytic activity	1400:1425	reduced hemolytic activity in a length-dependent manner	1400:1454	Strikingly, C-terminal truncation of hybrid peptides containing only the α-helical segment (PA-18) and shorter derivatives confer potent antimicrobial activity with reduced hemolytic activity in a length-dependent manner.
32499514	3	44	theme	well-known	680:689	arg1	peptides					699:706	the well-known natural peptides	676:706	the well-known natural peptides	676:706	In this study, we utilized key structural and physicochemical parameters, in combination with rational engineering, to design novel short α-helical hybrid peptides inspired by the well-known natural peptides, cathelicidin and aurein.
32499514	1	45	theme	Antimicrobial	165:177	arg1	AMPs					189:192	AMPs	189:192	AMPs	189:192	Antimicrobial peptides (AMPs) are promising alternatives to classical antibiotics for the treatment of drug-resistant infections.
32499514	1	45	theme	Antimicrobial	165:177	arg1	peptides					179:186	Antimicrobial peptides	165:186	Antimicrobial peptides (AMPs)	165:193	Antimicrobial peptides (AMPs) are promising alternatives to classical antibiotics for the treatment of drug-resistant infections.
32499514	1	45	theme	Antimicrobial	165:177	arg1	alternatives					209:220	promising alternatives	199:220	promising alternatives to classical antibiotics for the treatment of drug-resistant infections	199:292	Antimicrobial peptides (AMPs) are promising alternatives to classical antibiotics for the treatment of drug-resistant infections.
32499514	1	46	theme	promising	199:207	arg1	peptides					179:186	Antimicrobial peptides	165:186	Antimicrobial peptides (AMPs)	165:193	Antimicrobial peptides (AMPs) are promising alternatives to classical antibiotics for the treatment of drug-resistant infections.
32499514	1	46	theme	promising	199:207	arg1	alternatives					209:220	promising alternatives	199:220	promising alternatives to classical antibiotics for the treatment of drug-resistant infections	199:292	Antimicrobial peptides (AMPs) are promising alternatives to classical antibiotics for the treatment of drug-resistant infections.
32499514	11	47	theme	LPS-mediated	1918:1929	arg1	activation					1950:1959	dose-dependent, inhibiting, LPS-mediated Toll-like receptor activation	1890:1959	activation	1950:1959	Moreover, PA-13 showed an anti-inflammatory response via lipopolysaccharide (LPS) neutralization with dose-dependent, inhibiting, LPS-mediated Toll-like receptor activation.
32499514	12	48	theme	hybrid	2017:2022	arg1	peptide					2024:2030	a novel hybrid peptide	2009:2030	a novel hybrid peptide	2009:2030	This study revealed the therapeutic potency of a novel hybrid peptide, and supports the use of rational design in development of new antibacterial agents.
32499514	3	49	theme	natural	691:697	arg1	peptides					699:706	the well-known natural peptides	676:706	the well-known natural peptides	676:706	In this study, we utilized key structural and physicochemical parameters, in combination with rational engineering, to design novel short α-helical hybrid peptides inspired by the well-known natural peptides, cathelicidin and aurein.
32499514	3	50	theme	novel	626:630	arg1	cathelicidin					709:720	cathelicidin	709:720	cathelicidin	709:720	In this study, we utilized key structural and physicochemical parameters, in combination with rational engineering, to design novel short α-helical hybrid peptides inspired by the well-known natural peptides, cathelicidin and aurein.
32499514	3	50	theme	novel	626:630	arg1	aurein					726:731	aurein	726:731	aurein	726:731	In this study, we utilized key structural and physicochemical parameters, in combination with rational engineering, to design novel short α-helical hybrid peptides inspired by the well-known natural peptides, cathelicidin and aurein.
32499514	3	50	theme	novel	626:630	arg1	peptides					655:662	novel short α-helical hybrid peptides	626:662	novel short α-helical hybrid peptides inspired by the well-known natural peptides	626:706	In this study, we utilized key structural and physicochemical parameters, in combination with rational engineering, to design novel short α-helical hybrid peptides inspired by the well-known natural peptides, cathelicidin and aurein.
32499514	9	51	theme	antibacterial	1508:1520	arg1	activity					1522:1529	remarkable broad-spectrum antibacterial activity	1482:1529	remarkable broad-spectrum antibacterial activity	1482:1529	Among all, PA-13, showed remarkable broad-spectrum antibacterial activity, especially against Pseudomonas aeruginosa with no toxicity.
32499514	2	52	theme	desired	434:440	arg1	parameters					453:462	desired biological parameters	434:462	desired biological parameters	434:462	Due to their versatility and unlimited sequence space, AMPs can be rationally designed by modulating physicochemical determinants to favor desired biological parameters and turned into novel therapeutics.
32499514	8	53	theme	hybrid	1272:1277	arg1	peptides					1279:1286	hybrid peptides	1272:1286	hybrid peptides containing only the α-helical segment (PA-18)	1272:1332	Strikingly, C-terminal truncation of hybrid peptides containing only the α-helical segment (PA-18) and shorter derivatives confer potent antimicrobial activity with reduced hemolytic activity in a length-dependent manner.
32499514	12	54	theme	design	2066:2071	arg1	use					2050:2052	the use	2046:2052	the use of rational design in development of new antibacterial agents	2046:2114	This study revealed the therapeutic potency of a novel hybrid peptide, and supports the use of rational design in development of new antibacterial agents.
32499514	3	55	theme	short	632:636	arg1	cathelicidin					709:720	cathelicidin	709:720	cathelicidin	709:720	In this study, we utilized key structural and physicochemical parameters, in combination with rational engineering, to design novel short α-helical hybrid peptides inspired by the well-known natural peptides, cathelicidin and aurein.
32499514	3	55	theme	short	632:636	arg1	aurein					726:731	aurein	726:731	aurein	726:731	In this study, we utilized key structural and physicochemical parameters, in combination with rational engineering, to design novel short α-helical hybrid peptides inspired by the well-known natural peptides, cathelicidin and aurein.
32499514	3	55	theme	short	632:636	arg1	peptides					655:662	novel short α-helical hybrid peptides	626:662	novel short α-helical hybrid peptides inspired by the well-known natural peptides	626:706	In this study, we utilized key structural and physicochemical parameters, in combination with rational engineering, to design novel short α-helical hybrid peptides inspired by the well-known natural peptides, cathelicidin and aurein.
32499514	8	56	theme	α-helical	1308:1316	arg1	PA-18					1327:1331	PA-18	1327:1331	PA-18	1327:1331	Strikingly, C-terminal truncation of hybrid peptides containing only the α-helical segment (PA-18) and shorter derivatives confer potent antimicrobial activity with reduced hemolytic activity in a length-dependent manner.
32499514	8	56	theme	α-helical	1308:1316	arg1	segment					1318:1324	only the α-helical segment	1299:1324	only the α-helical segment (PA-18)	1299:1332	Strikingly, C-terminal truncation of hybrid peptides containing only the α-helical segment (PA-18) and shorter derivatives confer potent antimicrobial activity with reduced hemolytic activity in a length-dependent manner.
32499514	7	57	theme	antibacterial	1142:1154	arg1	activity					1156:1163	higher antibacterial activity	1135:1163	higher antibacterial activity	1135:1163	The results demonstrated that P7A3 had higher antibacterial activity than the parental peptides with unexpectedly high hemolytic activity.
32499514	11	58	theme	receptor	1941:1948	arg1	activation					1950:1959	dose-dependent, inhibiting, LPS-mediated Toll-like receptor activation	1890:1959	activation	1950:1959	Moreover, PA-13 showed an anti-inflammatory response via lipopolysaccharide (LPS) neutralization with dose-dependent, inhibiting, LPS-mediated Toll-like receptor activation.
32499514	3	59	used	utilized	518:525	arg2	we					515:516	we	515:516	we	515:516	In this study, we utilized key structural and physicochemical parameters, in combination with rational engineering, to design novel short α-helical hybrid peptides inspired by the well-known natural peptides, cathelicidin and aurein.
32499514	3	60	with	combination	577:587	arg1	engineering					603:613	rational engineering	594:613	rational engineering	594:613	In this study, we utilized key structural and physicochemical parameters, in combination with rational engineering, to design novel short α-helical hybrid peptides inspired by the well-known natural peptides, cathelicidin and aurein.
32499514	11	61	theme	dose-dependent	1890:1903	arg1	activation					1950:1959	dose-dependent, inhibiting, LPS-mediated Toll-like receptor activation	1890:1959	activation	1950:1959	Moreover, PA-13 showed an anti-inflammatory response via lipopolysaccharide (LPS) neutralization with dose-dependent, inhibiting, LPS-mediated Toll-like receptor activation.
32499514	11	62	dep	dose-dependent	1890:1903	arg1	inhibiting					1906:1915	inhibiting	1906:1915	inhibiting	1906:1915	Moreover, PA-13 showed an anti-inflammatory response via lipopolysaccharide (LPS) neutralization with dose-dependent, inhibiting, LPS-mediated Toll-like receptor activation.
32499514	4	63	theme	homologous	747:756	arg1	sequences					758:766	homologous sequences	747:766	homologous sequences	747:766	By comparing homologous sequences and abstracting the conserved residue type, sequence templates of cathelicidin (P0) and aurein (A0) were obtained.
32499514	12	64	theme	antibacterial	2095:2107	arg1	agents					2109:2114	new antibacterial agents	2091:2114	new antibacterial agents	2091:2114	This study revealed the therapeutic potency of a novel hybrid peptide, and supports the use of rational design in development of new antibacterial agents.
32499514	11	65	theme	lipopolysaccharide	1845:1862	arg1	neutralization					1870:1883	lipopolysaccharide (LPS) neutralization	1845:1883	lipopolysaccharide (LPS) neutralization with dose-dependent, inhibiting, LPS-mediated Toll-like receptor activation	1845:1959	Moreover, PA-13 showed an anti-inflammatory response via lipopolysaccharide (LPS) neutralization with dose-dependent, inhibiting, LPS-mediated Toll-like receptor activation.
32499514	8	66	theme	length-dependent	1432:1447	arg1	manner					1449:1454	a length-dependent manner	1430:1454	a length-dependent manner	1430:1454	Strikingly, C-terminal truncation of hybrid peptides containing only the α-helical segment (PA-18) and shorter derivatives confer potent antimicrobial activity with reduced hemolytic activity in a length-dependent manner.
32499514	1	67	theme	classical	225:233	arg1	antibiotics					235:245	classical antibiotics	225:245	classical antibiotics	225:245	Antimicrobial peptides (AMPs) are promising alternatives to classical antibiotics for the treatment of drug-resistant infections.
32499514	12	68	theme	therapeutic	1986:1996	arg1	potency					1998:2004	the therapeutic potency	1982:2004	the therapeutic potency of a novel hybrid peptide	1982:2030	This study revealed the therapeutic potency of a novel hybrid peptide, and supports the use of rational design in development of new antibacterial agents.
32499514	11	69	theme	anti-inflammatory	1814:1830	arg1	response					1832:1839	an anti-inflammatory response	1811:1839	an anti-inflammatory response	1811:1839	Moreover, PA-13 showed an anti-inflammatory response via lipopolysaccharide (LPS) neutralization with dose-dependent, inhibiting, LPS-mediated Toll-like receptor activation.
32499514	4	70	theme	cathelicidin	834:845	arg1	templates					821:829	sequence templates	812:829	sequence templates of cathelicidin (P0) and aurein (A0)	812:866	By comparing homologous sequences and abstracting the conserved residue type, sequence templates of cathelicidin (P0) and aurein (A0) were obtained.
32499514	4	71	theme	residue	798:804	arg1	type					806:809	the conserved residue type	784:809	the conserved residue type	784:809	By comparing homologous sequences and abstracting the conserved residue type, sequence templates of cathelicidin (P0) and aurein (A0) were obtained.
32499514	3	72	theme	physicochemical	546:560	arg1	parameters					562:571	key structural and physicochemical parameters	527:571	key structural and physicochemical parameters	527:571	In this study, we utilized key structural and physicochemical parameters, in combination with rational engineering, to design novel short α-helical hybrid peptides inspired by the well-known natural peptides, cathelicidin and aurein.
32499514	8	73	theme	potent	1365:1370	arg1	activity					1386:1393	potent antimicrobial activity	1365:1393	potent antimicrobial activity	1365:1393	Strikingly, C-terminal truncation of hybrid peptides containing only the α-helical segment (PA-18) and shorter derivatives confer potent antimicrobial activity with reduced hemolytic activity in a length-dependent manner.
32499514	6	74	theme	antimicrobial	1034:1046	arg1	activity					1048:1055	antimicrobial activity	1034:1055	antimicrobial activity	1034:1055	In order to enhance antimicrobial activity, a hybrid analog of P7A3 was designed.
32499514	7	75	contain	had	1131:1133	arg1	P7A3					1126:1129	P7A3	1126:1129	P7A3	1126:1129	The results demonstrated that P7A3 had higher antibacterial activity than the parental peptides with unexpectedly high hemolytic activity.
32499514	7	75	contain	had	1131:1133	arg2	activity					1156:1163	higher antibacterial activity	1135:1163	higher antibacterial activity	1135:1163	The results demonstrated that P7A3 had higher antibacterial activity than the parental peptides with unexpectedly high hemolytic activity.
32499514	0	76	dep	novel	2:6	arg1	designed					20:27	designed	20:27	designed	20:27	A novel, rationally designed, hybrid antimicrobial peptide, inspired by cathelicidin and aurein, exhibits membrane-active mechanisms against Pseudomonas aeruginosa.
32499514	0	76	dep	novel	2:6	arg1	antimicrobial					37:49	antimicrobial	37:49	antimicrobial	37:49	A novel, rationally designed, hybrid antimicrobial peptide, inspired by cathelicidin and aurein, exhibits membrane-active mechanisms against Pseudomonas aeruginosa.
32499514	9	77	theme	remarkable	1482:1491	arg1	activity					1522:1529	remarkable broad-spectrum antibacterial activity	1482:1529	remarkable broad-spectrum antibacterial activity	1482:1529	Among all, PA-13, showed remarkable broad-spectrum antibacterial activity, especially against Pseudomonas aeruginosa with no toxicity.
32499514	12	78	from	use	2050:2052	arg1	development					2076:2086	development	2076:2086	development of new antibacterial agents	2076:2114	This study revealed the therapeutic potency of a novel hybrid peptide, and supports the use of rational design in development of new antibacterial agents.
32499514	10	79	theme	penetration	1703:1713	arg1	activity					1715:1722	penetration activity	1703:1722	penetration activity	1703:1722	PA-13 maintained antimicrobial activity in the presence of physiological salts and displayed rapid binding and penetration activity which resulted in membrane depolarization and permeabilization.
32499514	9	80	theme	broad-spectrum	1493:1506	arg1	activity					1522:1529	remarkable broad-spectrum antibacterial activity	1482:1529	remarkable broad-spectrum antibacterial activity	1482:1529	Among all, PA-13, showed remarkable broad-spectrum antibacterial activity, especially against Pseudomonas aeruginosa with no toxicity.
32499514	3	81	theme	hybrid	648:653	arg1	cathelicidin					709:720	cathelicidin	709:720	cathelicidin	709:720	In this study, we utilized key structural and physicochemical parameters, in combination with rational engineering, to design novel short α-helical hybrid peptides inspired by the well-known natural peptides, cathelicidin and aurein.
32499514	3	81	theme	hybrid	648:653	arg1	aurein					726:731	aurein	726:731	aurein	726:731	In this study, we utilized key structural and physicochemical parameters, in combination with rational engineering, to design novel short α-helical hybrid peptides inspired by the well-known natural peptides, cathelicidin and aurein.
32499514	3	81	theme	hybrid	648:653	arg1	peptides					655:662	novel short α-helical hybrid peptides	626:662	novel short α-helical hybrid peptides inspired by the well-known natural peptides	626:706	In this study, we utilized key structural and physicochemical parameters, in combination with rational engineering, to design novel short α-helical hybrid peptides inspired by the well-known natural peptides, cathelicidin and aurein.
32499514	0	82	theme	novel	2:6	arg1	peptide					51:57	A novel, rationally designed, hybrid antimicrobial peptide	0:57	A novel, rationally designed, hybrid antimicrobial peptide	0:57	A novel, rationally designed, hybrid antimicrobial peptide, inspired by cathelicidin and aurein, exhibits membrane-active mechanisms against Pseudomonas aeruginosa.
32499514	10	83	theme	physiological	1651:1663	arg1	salts					1665:1669	physiological salts	1651:1669	physiological salts	1651:1669	PA-13 maintained antimicrobial activity in the presence of physiological salts and displayed rapid binding and penetration activity which resulted in membrane depolarization and permeabilization.
32747249	3	0	theme	long-chain	341:350	arg1	group					358:362	long-chain alkyl group	341:362	long-chain alkyl group on the surface of chitosan	341:389	In this work, functional chitosan membranes were fabricated by a one-step hydrolysis method through grafting long-chain alkyl group on the surface of chitosan to increase its hydrophobicity.
32747249	6	1	theme	wax	935:937	arg1	oil					939:941	wax oil	935:941	wax oil	935:941	The product purity could reach more than 98 % using one crystallization step under the optimal conditions, and in this case, adsorption capacity of FCM-C8 for wax oil was 478.9 mg/g.
32747249	5	2	theme	chitosan	681:688	arg1	membrane					690:697	Octyl-trimethoxysilane modified chitosan membrane	649:697	Octyl-trimethoxysilane modified chitosan membrane (FCM-C8)	649:706	Octyl-trimethoxysilane modified chitosan membrane (FCM-C8) showed excellent capability to intensify this purification process.
32747249	5	2	theme	chitosan	681:688	arg1	FCM-C8					700:705	FCM-C8	700:705	FCM-C8	700:705	Octyl-trimethoxysilane modified chitosan membrane (FCM-C8) showed excellent capability to intensify this purification process.
32747249	3	3	theme	alkyl	352:356	arg1	group					358:362	long-chain alkyl group	341:362	long-chain alkyl group on the surface of chitosan	341:389	In this work, functional chitosan membranes were fabricated by a one-step hydrolysis method through grafting long-chain alkyl group on the surface of chitosan to increase its hydrophobicity.
32747249	3	4	from	group	358:362	arg1	surface					371:377	the surface	367:377	the surface of chitosan	367:389	In this work, functional chitosan membranes were fabricated by a one-step hydrolysis method through grafting long-chain alkyl group on the surface of chitosan to increase its hydrophobicity.
32747249	6	5	theme	FCM-C8	924:929	arg1	capacity					912:919	adsorption capacity	901:919	adsorption capacity of FCM-C8 for wax oil	901:941	The product purity could reach more than 98 % using one crystallization step under the optimal conditions, and in this case, adsorption capacity of FCM-C8 for wax oil was 478.9 mg/g.
32747249	3	6	theme	chitosan	382:389	arg1	surface					371:377	the surface	367:377	the surface of chitosan	367:389	In this work, functional chitosan membranes were fabricated by a one-step hydrolysis method through grafting long-chain alkyl group on the surface of chitosan to increase its hydrophobicity.
32747249	6	7	theme	crystallization	832:846	arg1	step					848:851	one crystallization step	828:851	one crystallization step	828:851	The product purity could reach more than 98 % using one crystallization step under the optimal conditions, and in this case, adsorption capacity of FCM-C8 for wax oil was 478.9 mg/g.
32747249	4	8	theme	as-prepared	427:437	arg1	membranes					439:447	The as-prepared membranes	423:447	The as-prepared membranes	423:447	The as-prepared membranes were used to adsorb wax oil (i.e., the impurity components) in Artemisia annua L. and to avoid co-precipitation of wax oil along with artemisinin using the crystallization technique for purification.
32747249	4	9	used	used	454:457	arg2	membranes					439:447	The as-prepared membranes	423:447	The as-prepared membranes	423:447	The as-prepared membranes were used to adsorb wax oil (i.e., the impurity components) in Artemisia annua L. and to avoid co-precipitation of wax oil along with artemisinin using the crystallization technique for purification.
32747249	2	10	theme	leaves	152:157	arg1	complex					174:180	complex	174:180	complex	174:180	Since the leaves composition is complex, artemisinin purification faces great challenges.
32747249	2	10	theme	leaves	152:157	arg1	composition					159:169	the leaves composition	148:169	the leaves composition	148:169	Since the leaves composition is complex, artemisinin purification faces great challenges.
32747249	7	11	from	kinetics	987:994	arg1	FCM-C8					1024:1029	FCM-C8	1024:1029	FCM-C8	1024:1029	In addition, the adsorption kinetics and mechanism of wax oil on FCM-C8 were studied.
32747249	0	12	theme	functional	14:23	arg1	chitosan					25:32	Biodegradable functional chitosan	0:32	Biodegradable functional chitosan	0:32	Biodegradable functional chitosan membrane for enhancement of artemisinin purification.
32747249	4	13	theme	impurity	488:495	arg1	oil					473:475	wax oil	469:475	wax oil (i.e., the impurity components)	469:507	The as-prepared membranes were used to adsorb wax oil (i.e., the impurity components) in Artemisia annua L. and to avoid co-precipitation of wax oil along with artemisinin using the crystallization technique for purification.
32747249	4	13	theme	impurity	488:495	arg1	components					497:506	the impurity components	484:506	the impurity components	484:506	The as-prepared membranes were used to adsorb wax oil (i.e., the impurity components) in Artemisia annua L. and to avoid co-precipitation of wax oil along with artemisinin using the crystallization technique for purification.
32747249	3	14	theme	functional	246:255	arg1	membranes					266:274	functional chitosan membranes	246:274	functional chitosan membranes	246:274	In this work, functional chitosan membranes were fabricated by a one-step hydrolysis method through grafting long-chain alkyl group on the surface of chitosan to increase its hydrophobicity.
32747249	0	15	theme	Biodegradable	0:12	arg1	chitosan					25:32	Biodegradable functional chitosan	0:32	Biodegradable functional chitosan	0:32	Biodegradable functional chitosan membrane for enhancement of artemisinin purification.
32747249	4	16	dep	components	497:506	arg1	i.e.					478:481	i.e.	478:481	i.e.	478:481	The as-prepared membranes were used to adsorb wax oil (i.e., the impurity components) in Artemisia annua L. and to avoid co-precipitation of wax oil along with artemisinin using the crystallization technique for purification.
32747249	3	17	theme	chitosan	257:264	arg1	membranes					266:274	functional chitosan membranes	246:274	functional chitosan membranes	246:274	In this work, functional chitosan membranes were fabricated by a one-step hydrolysis method through grafting long-chain alkyl group on the surface of chitosan to increase its hydrophobicity.
32747249	2	18	theme	artemisinin	183:193	arg1	purification					195:206	artemisinin purification	183:206	artemisinin purification	183:206	Since the leaves composition is complex, artemisinin purification faces great challenges.
32747249	8	19	theme	multiple	1084:1091	arg1	components					1093:1102	multiple components	1084:1102	multiple components in wax oil	1084:1113	The membrane can simultaneously adsorb multiple components in wax oil through interactions like electrostatic forces, hydrogen bondings.
32747249	4	20	theme	oil	568:570	arg1	co-precipitation					544:559	co-precipitation	544:559	co-precipitation of wax oil along with artemisinin using the crystallization technique for purification	544:646	The as-prepared membranes were used to adsorb wax oil (i.e., the impurity components) in Artemisia annua L. and to avoid co-precipitation of wax oil along with artemisinin using the crystallization technique for purification.
32747249	3	21	theme	one-step	297:304	arg1	method					317:322	a one-step hydrolysis method	295:322	a one-step hydrolysis method	295:322	In this work, functional chitosan membranes were fabricated by a one-step hydrolysis method through grafting long-chain alkyl group on the surface of chitosan to increase its hydrophobicity.
32747249	1	22	theme	Artemisia	123:131	arg1	L					139:139	Artemisia annua L	123:139	Artemisia annua L	123:139	Artemisinin is mainly derived from Artemisia annua L.
32747249	4	23	theme	wax	564:566	arg1	oil					568:570	wax oil	564:570	wax oil	564:570	The as-prepared membranes were used to adsorb wax oil (i.e., the impurity components) in Artemisia annua L. and to avoid co-precipitation of wax oil along with artemisinin using the crystallization technique for purification.
32747249	4	24	theme	wax	469:471	arg1	oil					473:475	wax oil	469:475	wax oil (i.e., the impurity components)	469:507	The as-prepared membranes were used to adsorb wax oil (i.e., the impurity components) in Artemisia annua L. and to avoid co-precipitation of wax oil along with artemisinin using the crystallization technique for purification.
32747249	4	24	theme	wax	469:471	arg1	components					497:506	the impurity components	484:506	the impurity components	484:506	The as-prepared membranes were used to adsorb wax oil (i.e., the impurity components) in Artemisia annua L. and to avoid co-precipitation of wax oil along with artemisinin using the crystallization technique for purification.
32747249	7	25	from	mechanism	1000:1008	arg1	FCM-C8					1024:1029	FCM-C8	1024:1029	FCM-C8	1024:1029	In addition, the adsorption kinetics and mechanism of wax oil on FCM-C8 were studied.
32747249	5	26	theme	Octyl-trimethoxysilane	649:670	arg1	membrane					690:697	Octyl-trimethoxysilane modified chitosan membrane	649:697	Octyl-trimethoxysilane modified chitosan membrane (FCM-C8)	649:706	Octyl-trimethoxysilane modified chitosan membrane (FCM-C8) showed excellent capability to intensify this purification process.
32747249	5	26	theme	Octyl-trimethoxysilane	649:670	arg1	FCM-C8					700:705	FCM-C8	700:705	FCM-C8	700:705	Octyl-trimethoxysilane modified chitosan membrane (FCM-C8) showed excellent capability to intensify this purification process.
32747249	1	27	attach	derived	110:116	arg1	L					139:139	Artemisia annua L	123:139	Artemisia annua L	123:139	Artemisinin is mainly derived from Artemisia annua L.
32747249	1	27	attach	derived	110:116	arg2	Artemisinin					88:98	Artemisinin	88:98	Artemisinin	88:98	Artemisinin is mainly derived from Artemisia annua L.
32747249	5	28	theme	purification	754:765	arg1	process					767:773	this purification process	749:773	this purification process	749:773	Octyl-trimethoxysilane modified chitosan membrane (FCM-C8) showed excellent capability to intensify this purification process.
32747249	7	29	theme	oil	1017:1019	arg1	mechanism					1000:1008	mechanism	1000:1008	mechanism	1000:1008	In addition, the adsorption kinetics and mechanism of wax oil on FCM-C8 were studied.
32747249	7	29	theme	oil	1017:1019	arg1	kinetics					987:994	adsorption kinetics	976:994	adsorption kinetics	976:994	In addition, the adsorption kinetics and mechanism of wax oil on FCM-C8 were studied.
32747249	0	30	theme	artemisinin	62:72	arg1	purification					74:85	artemisinin purification	62:85	artemisinin purification	62:85	Biodegradable functional chitosan membrane for enhancement of artemisinin purification.
32747249	4	31	dep	Artemisia	512:520	arg1	L.					528:529	Artemisia annua L.	512:529	Artemisia annua L.	512:529	The as-prepared membranes were used to adsorb wax oil (i.e., the impurity components) in Artemisia annua L. and to avoid co-precipitation of wax oil along with artemisinin using the crystallization technique for purification.
32747249	5	32	theme	excellent	715:723	arg1	capability					725:734	excellent capability	715:734	excellent capability to intensify this purification process	715:773	Octyl-trimethoxysilane modified chitosan membrane (FCM-C8) showed excellent capability to intensify this purification process.
32747249	1	33	theme	annua	133:137	arg1	L					139:139	Artemisia annua L	123:139	Artemisia annua L	123:139	Artemisinin is mainly derived from Artemisia annua L.
32747249	8	34	theme	wax	1107:1109	arg1	oil					1111:1113	wax oil	1107:1113	wax oil	1107:1113	The membrane can simultaneously adsorb multiple components in wax oil through interactions like electrostatic forces, hydrogen bondings.
32747249	5	35	theme	modified	672:679	arg1	membrane					690:697	Octyl-trimethoxysilane modified chitosan membrane	649:697	Octyl-trimethoxysilane modified chitosan membrane (FCM-C8)	649:706	Octyl-trimethoxysilane modified chitosan membrane (FCM-C8) showed excellent capability to intensify this purification process.
32747249	5	35	theme	modified	672:679	arg1	FCM-C8					700:705	FCM-C8	700:705	FCM-C8	700:705	Octyl-trimethoxysilane modified chitosan membrane (FCM-C8) showed excellent capability to intensify this purification process.
32747249	3	36	theme	hydrolysis	306:315	arg1	method					317:322	a one-step hydrolysis method	295:322	a one-step hydrolysis method	295:322	In this work, functional chitosan membranes were fabricated by a one-step hydrolysis method through grafting long-chain alkyl group on the surface of chitosan to increase its hydrophobicity.
32747249	7	37	theme	wax	1013:1015	arg1	oil					1017:1019	wax oil	1013:1019	wax oil	1013:1019	In addition, the adsorption kinetics and mechanism of wax oil on FCM-C8 were studied.
32747249	4	38	theme	crystallization	605:619	arg1	technique					621:629	the crystallization technique	601:629	the crystallization technique for purification	601:646	The as-prepared membranes were used to adsorb wax oil (i.e., the impurity components) in Artemisia annua L. and to avoid co-precipitation of wax oil along with artemisinin using the crystallization technique for purification.
32747249	6	39	from	mg/g	953:956	arg1	case					895:898	this case	890:898	this case	890:898	The product purity could reach more than 98 % using one crystallization step under the optimal conditions, and in this case, adsorption capacity of FCM-C8 for wax oil was 478.9 mg/g.
32747249	2	40	theme	great	214:218	arg1	challenges					220:229	great challenges	214:229	great challenges	214:229	Since the leaves composition is complex, artemisinin purification faces great challenges.
32747249	6	41	theme	product	780:786	arg1	purity					788:793	The product purity	776:793	The product purity	776:793	The product purity could reach more than 98 % using one crystallization step under the optimal conditions, and in this case, adsorption capacity of FCM-C8 for wax oil was 478.9 mg/g.
32747249	8	42	from	components	1093:1102	arg1	oil					1111:1113	wax oil	1107:1113	wax oil	1107:1113	The membrane can simultaneously adsorb multiple components in wax oil through interactions like electrostatic forces, hydrogen bondings.
32747249	8	43	theme	electrostatic	1141:1153	arg1	forces					1155:1160	electrostatic forces	1141:1160	electrostatic forces	1141:1160	The membrane can simultaneously adsorb multiple components in wax oil through interactions like electrostatic forces, hydrogen bondings.
32747249	6	44	theme	adsorption	901:910	arg1	capacity					912:919	adsorption capacity	901:919	adsorption capacity of FCM-C8 for wax oil	901:941	The product purity could reach more than 98 % using one crystallization step under the optimal conditions, and in this case, adsorption capacity of FCM-C8 for wax oil was 478.9 mg/g.
32747249	6	45	theme	optimal	863:869	arg1	conditions					871:880	the optimal conditions	859:880	the optimal conditions	859:880	The product purity could reach more than 98 % using one crystallization step under the optimal conditions, and in this case, adsorption capacity of FCM-C8 for wax oil was 478.9 mg/g.
32747249	0	46	theme	purification	74:85	arg1	enhancement					47:57	enhancement	47:57	enhancement of artemisinin purification	47:85	Biodegradable functional chitosan membrane for enhancement of artemisinin purification.
32747249	7	47	theme	adsorption	976:985	arg1	kinetics					987:994	adsorption kinetics	976:994	adsorption kinetics	976:994	In addition, the adsorption kinetics and mechanism of wax oil on FCM-C8 were studied.
32747249	8	48	theme	hydrogen	1163:1170	arg1	bondings					1172:1179	hydrogen bondings	1163:1179	hydrogen bondings	1163:1179	The membrane can simultaneously adsorb multiple components in wax oil through interactions like electrostatic forces, hydrogen bondings.
32600688	6	0	theme	in	961:962	arg1	performance					970:980	in vitro performance	961:980	in vitro performance	961:980	The coating surface was characterized by microscopic examination, and in vitro performance was evaluated after immersion in phosphate-buffered saline (PBS) solution, simulated body fluid (SBF), and cell culture medium, to analyze coating degradation, antibiotics release, cell attachment (ST-2 stromal cells), and antibacterial (Escherichia coli and Staphylococcus aureus) properties.
32600688	4	1	theme	work	591:594	arg1	aim					579:581	The aim	575:581	The aim of this work	575:594	The aim of this work is to generate a degradable coating with antibacterial properties for stainless steel to be used in implants/medical devices.
32600688	3	2	theme	osseointegration	520:535	arg1	absence					542:548	absence	542:548	absence of antibacterial effect	542:572	However, its use as permanent implant in orthopedic surgery is conditioned due to its limited corrosion resistance in physiological media, lack of osseointegration, and absence of antibacterial effect.
32600688	3	2	theme	osseointegration	520:535	arg1	lack					512:515	lack	512:515	lack of osseointegration	512:535	However, its use as permanent implant in orthopedic surgery is conditioned due to its limited corrosion resistance in physiological media, lack of osseointegration, and absence of antibacterial effect.
32600688	3	2	theme	osseointegration	520:535	arg1	resistance					477:486	its limited corrosion resistance	455:486	its limited corrosion resistance in physiological media	455:509	However, its use as permanent implant in orthopedic surgery is conditioned due to its limited corrosion resistance in physiological media, lack of osseointegration, and absence of antibacterial effect.
32600688	1	3	theme	several	151:157	arg1	applications					170:181	several orthopedic applications	151:181	several orthopedic applications as fixation elements for the stabilization of fractures or as prostheses	151:254	Metals are used in several orthopedic applications as fixation elements for the stabilization of fractures or as prostheses.
32600688	3	4	theme	effect	567:572	arg1	absence					542:548	absence	542:548	absence of antibacterial effect	542:572	However, its use as permanent implant in orthopedic surgery is conditioned due to its limited corrosion resistance in physiological media, lack of osseointegration, and absence of antibacterial effect.
32600688	3	4	theme	effect	567:572	arg1	lack					512:515	lack	512:515	lack of osseointegration	512:535	However, its use as permanent implant in orthopedic surgery is conditioned due to its limited corrosion resistance in physiological media, lack of osseointegration, and absence of antibacterial effect.
32600688	3	4	theme	effect	567:572	arg1	resistance					477:486	its limited corrosion resistance	455:486	its limited corrosion resistance in physiological media	455:509	However, its use as permanent implant in orthopedic surgery is conditioned due to its limited corrosion resistance in physiological media, lack of osseointegration, and absence of antibacterial effect.
32600688	6	5	theme	microscopic	932:942	arg1	examination					944:954	microscopic examination	932:954	microscopic examination	932:954	The coating surface was characterized by microscopic examination, and in vitro performance was evaluated after immersion in phosphate-buffered saline (PBS) solution, simulated body fluid (SBF), and cell culture medium, to analyze coating degradation, antibiotics release, cell attachment (ST-2 stromal cells), and antibacterial (Escherichia coli and Staphylococcus aureus) properties.
32600688	6	6	theme	stromal	1185:1191	arg1	attachment					1168:1177	cell attachment	1163:1177	cell attachment (ST-2 stromal cells)	1163:1198	The coating surface was characterized by microscopic examination, and in vitro performance was evaluated after immersion in phosphate-buffered saline (PBS) solution, simulated body fluid (SBF), and cell culture medium, to analyze coating degradation, antibiotics release, cell attachment (ST-2 stromal cells), and antibacterial (Escherichia coli and Staphylococcus aureus) properties.
32600688	6	6	theme	stromal	1185:1191	arg1	cells					1193:1197	ST-2 stromal cells	1180:1197	ST-2 stromal cells	1180:1197	The coating surface was characterized by microscopic examination, and in vitro performance was evaluated after immersion in phosphate-buffered saline (PBS) solution, simulated body fluid (SBF), and cell culture medium, to analyze coating degradation, antibiotics release, cell attachment (ST-2 stromal cells), and antibacterial (Escherichia coli and Staphylococcus aureus) properties.
32600688	10	7	from	culture	1658:1664	arg1	proliferation					1633:1645	the cell proliferation	1624:1645	meanwhile the cell proliferation (at 7 days culture)	1614:1665	The gentamicin release led to excellent antibacterial behavior at 24 h, meanwhile the cell proliferation (at 7 days culture) was not inhibited.
32600688	2	8	theme	grade	345:349	arg1	SS					368:369	SS	368:369	SS	368:369	One of the most common orthopedic biomaterials in many developing countries is surgical grade stainless steel (SS).
32600688	2	8	theme	grade	345:349	arg1	steel					361:365	surgical grade stainless steel	336:365	surgical grade stainless steel (SS)	336:370	One of the most common orthopedic biomaterials in many developing countries is surgical grade stainless steel (SS).
32600688	9	9	theme	chitosan-gelatin	1477:1492	arg1	coatings					1494:1501	the chitosan-gelatin coatings	1473:1501	the chitosan-gelatin coatings	1473:1501	The degradation of the chitosan-gelatin coatings was evident after 7 days of immersion.
32600688	6	10	theme	phosphate-buffered	1015:1032	arg1	PBS					1042:1044	PBS	1042:1044	PBS	1042:1044	The coating surface was characterized by microscopic examination, and in vitro performance was evaluated after immersion in phosphate-buffered saline (PBS) solution, simulated body fluid (SBF), and cell culture medium, to analyze coating degradation, antibiotics release, cell attachment (ST-2 stromal cells), and antibacterial (Escherichia coli and Staphylococcus aureus) properties.
32600688	6	10	theme	phosphate-buffered	1015:1032	arg1	saline					1034:1039	phosphate-buffered saline	1015:1039	phosphate-buffered saline (PBS) solution	1015:1054	The coating surface was characterized by microscopic examination, and in vitro performance was evaluated after immersion in phosphate-buffered saline (PBS) solution, simulated body fluid (SBF), and cell culture medium, to analyze coating degradation, antibiotics release, cell attachment (ST-2 stromal cells), and antibacterial (Escherichia coli and Staphylococcus aureus) properties.
32600688	6	11	theme	cell	1163:1166	arg1	attachment					1168:1177	cell attachment	1163:1177	cell attachment (ST-2 stromal cells)	1163:1198	The coating surface was characterized by microscopic examination, and in vitro performance was evaluated after immersion in phosphate-buffered saline (PBS) solution, simulated body fluid (SBF), and cell culture medium, to analyze coating degradation, antibiotics release, cell attachment (ST-2 stromal cells), and antibacterial (Escherichia coli and Staphylococcus aureus) properties.
32600688	6	11	theme	cell	1163:1166	arg1	cells					1193:1197	ST-2 stromal cells	1180:1197	ST-2 stromal cells	1180:1197	The coating surface was characterized by microscopic examination, and in vitro performance was evaluated after immersion in phosphate-buffered saline (PBS) solution, simulated body fluid (SBF), and cell culture medium, to analyze coating degradation, antibiotics release, cell attachment (ST-2 stromal cells), and antibacterial (Escherichia coli and Staphylococcus aureus) properties.
32600688	4	12	theme	antibacterial	637:649	arg1	properties					651:660	antibacterial properties	637:660	antibacterial properties for stainless steel	637:680	The aim of this work is to generate a degradable coating with antibacterial properties for stainless steel to be used in implants/medical devices.
32600688	5	13	theme	steel	877:881	arg1	plates					883:888	surgical grade stainless steel plates	852:888	surgical grade stainless steel plates	852:888	The coating is composed of a biopolymer/silica-gentamicin nanoparticles composite obtained by electrophoretic deposition (EPD) on surgical grade stainless steel plates.
32600688	5	14	theme	surgical	852:859	arg1	plates					883:888	surgical grade stainless steel plates	852:888	surgical grade stainless steel plates	852:888	The coating is composed of a biopolymer/silica-gentamicin nanoparticles composite obtained by electrophoretic deposition (EPD) on surgical grade stainless steel plates.
32600688	1	15	theme	prostheses	245:254	arg1	stabilization					212:224	the stabilization	208:224	the stabilization of fractures or as prostheses	208:254	Metals are used in several orthopedic applications as fixation elements for the stabilization of fractures or as prostheses.
32600688	2	16	theme	orthopedic	280:289	arg1	biomaterials					291:302	the most common orthopedic biomaterials	264:302	the most common orthopedic biomaterials in many developing countries	264:331	One of the most common orthopedic biomaterials in many developing countries is surgical grade stainless steel (SS).
32600688	1	17	theme	orthopedic	159:168	arg1	applications					170:181	several orthopedic applications	151:181	several orthopedic applications as fixation elements for the stabilization of fractures or as prostheses	151:254	Metals are used in several orthopedic applications as fixation elements for the stabilization of fractures or as prostheses.
32600688	6	18	theme	body	1067:1070	arg1	SBF					1079:1081	SBF	1079:1081	SBF	1079:1081	The coating surface was characterized by microscopic examination, and in vitro performance was evaluated after immersion in phosphate-buffered saline (PBS) solution, simulated body fluid (SBF), and cell culture medium, to analyze coating degradation, antibiotics release, cell attachment (ST-2 stromal cells), and antibacterial (Escherichia coli and Staphylococcus aureus) properties.
32600688	6	18	theme	body	1067:1070	arg1	fluid					1072:1076	simulated body fluid	1057:1076	simulated body fluid (SBF)	1057:1082	The coating surface was characterized by microscopic examination, and in vitro performance was evaluated after immersion in phosphate-buffered saline (PBS) solution, simulated body fluid (SBF), and cell culture medium, to analyze coating degradation, antibiotics release, cell attachment (ST-2 stromal cells), and antibacterial (Escherichia coli and Staphylococcus aureus) properties.
32600688	1	19	theme	fractures	229:237	arg1	stabilization					212:224	the stabilization	208:224	the stabilization of fractures or as prostheses	208:254	Metals are used in several orthopedic applications as fixation elements for the stabilization of fractures or as prostheses.
32600688	7	20	theme	substrate	1350:1358	arg1	surface					1332:1338	the surface	1328:1338	the surface of the SS substrate	1328:1358	EPD coatings were uniform and covered homogeneously the surface of the SS substrate.
32600688	11	21	theme	early	1812:1816	arg1	times					1831:1835	early implantation times	1812:1835	early implantation times	1812:1835	The results show that the coating system exhibits promising behavior which could contribute to prevent hospital infections at early implantation times.
32600688	2	22	theme	developing	312:321	arg1	countries					323:331	many developing countries	307:331	many developing countries	307:331	One of the most common orthopedic biomaterials in many developing countries is surgical grade stainless steel (SS).
32600688	5	23	theme	biopolymer/silica-gentamicin	751:778	arg1	composite					794:802	a biopolymer/silica-gentamicin nanoparticles composite	749:802	a biopolymer/silica-gentamicin nanoparticles composite obtained by electrophoretic deposition (EPD) on surgical grade stainless steel plates	749:888	The coating is composed of a biopolymer/silica-gentamicin nanoparticles composite obtained by electrophoretic deposition (EPD) on surgical grade stainless steel plates.
32600688	7	24	theme	SS	1347:1348	arg1	substrate					1350:1358	the SS substrate	1343:1358	the SS substrate	1343:1358	EPD coatings were uniform and covered homogeneously the surface of the SS substrate.
32600688	3	25	theme	permanent	393:401	arg1	implant					403:409	permanent implant	393:409	permanent implant in orthopedic surgery	393:431	However, its use as permanent implant in orthopedic surgery is conditioned due to its limited corrosion resistance in physiological media, lack of osseointegration, and absence of antibacterial effect.
32600688	0	26	theme	surgical	100:107	arg1	grade					109:113	surgical grade	100:113	surgical grade	100:113	Functional behavior of chitosan/gelatin/silica-gentamicin coatings by electrophoretic deposition on surgical grade stainless steel.
32600688	0	27	theme	Functional	0:9	arg1	behavior					11:18	Functional behavior	0:18	Functional behavior of chitosan/gelatin/silica-gentamicin coatings by electrophoretic deposition on surgical grade	0:113	Functional behavior of chitosan/gelatin/silica-gentamicin coatings by electrophoretic deposition on surgical grade stainless steel.
32600688	8	28	theme	nanoparticles	1404:1416	arg1	homogeneous					1422:1432	homogeneous	1422:1432	homogeneous	1422:1432	Also the distribution of silica-gentamicin nanoparticles was homogeneous on the coated area.
32600688	8	28	theme	nanoparticles	1404:1416	arg1	distribution					1370:1381	the distribution	1366:1381	the distribution of silica-gentamicin nanoparticles	1366:1416	Also the distribution of silica-gentamicin nanoparticles was homogeneous on the coated area.
32600688	5	29	theme	grade	861:865	arg1	plates					883:888	surgical grade stainless steel plates	852:888	surgical grade stainless steel plates	852:888	The coating is composed of a biopolymer/silica-gentamicin nanoparticles composite obtained by electrophoretic deposition (EPD) on surgical grade stainless steel plates.
32600688	2	30	from	biomaterials	291:302	arg1	countries					323:331	many developing countries	307:331	many developing countries	307:331	One of the most common orthopedic biomaterials in many developing countries is surgical grade stainless steel (SS).
32600688	3	31	theme	limited	459:465	arg1	resistance					477:486	its limited corrosion resistance	455:486	its limited corrosion resistance in physiological media	455:509	However, its use as permanent implant in orthopedic surgery is conditioned due to its limited corrosion resistance in physiological media, lack of osseointegration, and absence of antibacterial effect.
32600688	0	32	theme	coatings	58:65	arg1	behavior					11:18	Functional behavior	0:18	Functional behavior of chitosan/gelatin/silica-gentamicin coatings by electrophoretic deposition on surgical grade	0:113	Functional behavior of chitosan/gelatin/silica-gentamicin coatings by electrophoretic deposition on surgical grade stainless steel.
32600688	6	33	theme	coating	895:901	arg1	surface					903:909	The coating surface	891:909	The coating surface	891:909	The coating surface was characterized by microscopic examination, and in vitro performance was evaluated after immersion in phosphate-buffered saline (PBS) solution, simulated body fluid (SBF), and cell culture medium, to analyze coating degradation, antibiotics release, cell attachment (ST-2 stromal cells), and antibacterial (Escherichia coli and Staphylococcus aureus) properties.
32600688	11	34	theme	coating	1712:1718	arg1	system					1720:1725	the coating system	1708:1725	the coating system	1708:1725	The results show that the coating system exhibits promising behavior which could contribute to prevent hospital infections at early implantation times.
32600688	0	35	theme	electrophoretic	70:84	arg1	deposition					86:95	electrophoretic deposition	70:95	electrophoretic deposition on surgical grade	70:113	Functional behavior of chitosan/gelatin/silica-gentamicin coatings by electrophoretic deposition on surgical grade stainless steel.
32600688	6	36	theme	antibiotics	1142:1152	arg1	release					1154:1160	antibiotics release	1142:1160	antibiotics release	1142:1160	The coating surface was characterized by microscopic examination, and in vitro performance was evaluated after immersion in phosphate-buffered saline (PBS) solution, simulated body fluid (SBF), and cell culture medium, to analyze coating degradation, antibiotics release, cell attachment (ST-2 stromal cells), and antibacterial (Escherichia coli and Staphylococcus aureus) properties.
32600688	10	37	theme	gentamicin	1546:1555	arg1	release					1557:1563	The gentamicin release	1542:1563	The gentamicin release	1542:1563	The gentamicin release led to excellent antibacterial behavior at 24 h, meanwhile the cell proliferation (at 7 days culture) was not inhibited.
32600688	3	38	theme	physiological	491:503	arg1	media					505:509	physiological media	491:509	physiological media	491:509	However, its use as permanent implant in orthopedic surgery is conditioned due to its limited corrosion resistance in physiological media, lack of osseointegration, and absence of antibacterial effect.
32600688	4	39	theme	degradable	613:622	arg1	coating					624:630	a degradable coating	611:630	a degradable coating with antibacterial properties for stainless steel to be used in implants/medical devices	611:719	The aim of this work is to generate a degradable coating with antibacterial properties for stainless steel to be used in implants/medical devices.
32600688	10	40	theme	excellent	1572:1580	arg1	behavior					1596:1603	excellent antibacterial behavior	1572:1603	excellent antibacterial behavior at 24 h, meanwhile the cell proliferation (at 7 days culture) was not inhibited	1572:1683	The gentamicin release led to excellent antibacterial behavior at 24 h, meanwhile the cell proliferation (at 7 days culture) was not inhibited.
32600688	6	41	dep	properties	1264:1273	arg1	coli					1232:1235	Escherichia coli	1220:1235	Escherichia coli	1220:1235	The coating surface was characterized by microscopic examination, and in vitro performance was evaluated after immersion in phosphate-buffered saline (PBS) solution, simulated body fluid (SBF), and cell culture medium, to analyze coating degradation, antibiotics release, cell attachment (ST-2 stromal cells), and antibacterial (Escherichia coli and Staphylococcus aureus) properties.
32600688	6	41	dep	properties	1264:1273	arg1	aureus					1256:1261	Staphylococcus aureus	1241:1261	Staphylococcus aureus	1241:1261	The coating surface was characterized by microscopic examination, and in vitro performance was evaluated after immersion in phosphate-buffered saline (PBS) solution, simulated body fluid (SBF), and cell culture medium, to analyze coating degradation, antibiotics release, cell attachment (ST-2 stromal cells), and antibacterial (Escherichia coli and Staphylococcus aureus) properties.
32600688	3	42	theme	antibacterial	553:565	arg1	effect					567:572	antibacterial effect	553:572	antibacterial effect	553:572	However, its use as permanent implant in orthopedic surgery is conditioned due to its limited corrosion resistance in physiological media, lack of osseointegration, and absence of antibacterial effect.
32600688	6	43	dep	in	961:962	arg1	vitro					964:968	vitro	964:968	vitro	964:968	The coating surface was characterized by microscopic examination, and in vitro performance was evaluated after immersion in phosphate-buffered saline (PBS) solution, simulated body fluid (SBF), and cell culture medium, to analyze coating degradation, antibiotics release, cell attachment (ST-2 stromal cells), and antibacterial (Escherichia coli and Staphylococcus aureus) properties.
32600688	2	44	theme	many	307:310	arg1	countries					323:331	many developing countries	307:331	many developing countries	307:331	One of the most common orthopedic biomaterials in many developing countries is surgical grade stainless steel (SS).
32600688	6	45	theme	ST-2	1180:1183	arg1	attachment					1168:1177	cell attachment	1163:1177	cell attachment (ST-2 stromal cells)	1163:1198	The coating surface was characterized by microscopic examination, and in vitro performance was evaluated after immersion in phosphate-buffered saline (PBS) solution, simulated body fluid (SBF), and cell culture medium, to analyze coating degradation, antibiotics release, cell attachment (ST-2 stromal cells), and antibacterial (Escherichia coli and Staphylococcus aureus) properties.
32600688	6	45	theme	ST-2	1180:1183	arg1	cells					1193:1197	ST-2 stromal cells	1180:1197	ST-2 stromal cells	1180:1197	The coating surface was characterized by microscopic examination, and in vitro performance was evaluated after immersion in phosphate-buffered saline (PBS) solution, simulated body fluid (SBF), and cell culture medium, to analyze coating degradation, antibiotics release, cell attachment (ST-2 stromal cells), and antibacterial (Escherichia coli and Staphylococcus aureus) properties.
32600688	4	46	theme	stainless	666:674	arg1	steel					676:680	stainless steel	666:680	stainless steel	666:680	The aim of this work is to generate a degradable coating with antibacterial properties for stainless steel to be used in implants/medical devices.
32600688	2	47	theme	stainless	351:359	arg1	SS					368:369	SS	368:369	SS	368:369	One of the most common orthopedic biomaterials in many developing countries is surgical grade stainless steel (SS).
32600688	2	47	theme	stainless	351:359	arg1	steel					361:365	surgical grade stainless steel	336:365	surgical grade stainless steel (SS)	336:370	One of the most common orthopedic biomaterials in many developing countries is surgical grade stainless steel (SS).
32600688	6	48	theme	saline	1034:1039	arg1	solution					1047:1054	phosphate-buffered saline (PBS) solution	1015:1054	phosphate-buffered saline (PBS) solution	1015:1054	The coating surface was characterized by microscopic examination, and in vitro performance was evaluated after immersion in phosphate-buffered saline (PBS) solution, simulated body fluid (SBF), and cell culture medium, to analyze coating degradation, antibiotics release, cell attachment (ST-2 stromal cells), and antibacterial (Escherichia coli and Staphylococcus aureus) properties.
32600688	10	49	theme	cell	1628:1631	arg1	proliferation					1633:1645	the cell proliferation	1624:1645	meanwhile the cell proliferation (at 7 days culture)	1614:1665	The gentamicin release led to excellent antibacterial behavior at 24 h, meanwhile the cell proliferation (at 7 days culture) was not inhibited.
32600688	3	50	from	lack	512:515	arg1	media					505:509	physiological media	491:509	physiological media	491:509	However, its use as permanent implant in orthopedic surgery is conditioned due to its limited corrosion resistance in physiological media, lack of osseointegration, and absence of antibacterial effect.
32600688	2	51	theme	surgical	336:343	arg1	SS					368:369	SS	368:369	SS	368:369	One of the most common orthopedic biomaterials in many developing countries is surgical grade stainless steel (SS).
32600688	2	51	theme	surgical	336:343	arg1	steel					361:365	surgical grade stainless steel	336:365	surgical grade stainless steel (SS)	336:370	One of the most common orthopedic biomaterials in many developing countries is surgical grade stainless steel (SS).
32600688	5	52	theme	stainless	867:875	arg1	plates					883:888	surgical grade stainless steel plates	852:888	surgical grade stainless steel plates	852:888	The coating is composed of a biopolymer/silica-gentamicin nanoparticles composite obtained by electrophoretic deposition (EPD) on surgical grade stainless steel plates.
32600688	1	53	used	used	143:146	arg2	Metals					132:137	Metals	132:137	Metals	132:137	Metals are used in several orthopedic applications as fixation elements for the stabilization of fractures or as prostheses.
32600688	10	54	dep	meanwhile	1614:1622	arg1	proliferation					1633:1645	the cell proliferation	1624:1645	meanwhile the cell proliferation (at 7 days culture)	1614:1665	The gentamicin release led to excellent antibacterial behavior at 24 h, meanwhile the cell proliferation (at 7 days culture) was not inhibited.
32600688	4	55	with	coating	624:630	arg1	properties					651:660	antibacterial properties	637:660	antibacterial properties for stainless steel	637:680	The aim of this work is to generate a degradable coating with antibacterial properties for stainless steel to be used in implants/medical devices.
32600688	1	56	theme	as	242:243	arg1	prostheses					245:254	as prostheses	242:254	as prostheses	242:254	Metals are used in several orthopedic applications as fixation elements for the stabilization of fractures or as prostheses.
32600688	10	57	theme	7 days	1651:1656	arg1	culture					1658:1664	7 days culture	1651:1664	7 days culture	1651:1664	The gentamicin release led to excellent antibacterial behavior at 24 h, meanwhile the cell proliferation (at 7 days culture) was not inhibited.
32600688	10	58	dep	behavior	1596:1603	arg1	inhibited					1675:1683	inhibited	1675:1683	was not inhibited	1667:1683	The gentamicin release led to excellent antibacterial behavior at 24 h, meanwhile the cell proliferation (at 7 days culture) was not inhibited.
32600688	2	59	theme	biomaterials	291:302	arg1	One					257:259	One	257:259	One	257:259	One of the most common orthopedic biomaterials in many developing countries is surgical grade stainless steel (SS).
32600688	2	59	theme	biomaterials	291:302	arg1	biomaterials					291:302	the most common orthopedic biomaterials	264:302	the most common orthopedic biomaterials in many developing countries	264:331	One of the most common orthopedic biomaterials in many developing countries is surgical grade stainless steel (SS).
32600688	9	60	theme	immersion	1531:1539	arg1	7 days					1521:1526	7 days	1521:1526	7 days of immersion	1521:1539	The degradation of the chitosan-gelatin coatings was evident after 7 days of immersion.
32600688	6	61	theme	antibacterial	1205:1217	arg1	properties					1264:1273	antibacterial (Escherichia coli and Staphylococcus aureus) properties	1205:1273	antibacterial (Escherichia coli and Staphylococcus aureus) properties	1205:1273	The coating surface was characterized by microscopic examination, and in vitro performance was evaluated after immersion in phosphate-buffered saline (PBS) solution, simulated body fluid (SBF), and cell culture medium, to analyze coating degradation, antibiotics release, cell attachment (ST-2 stromal cells), and antibacterial (Escherichia coli and Staphylococcus aureus) properties.
32600688	11	62	theme	hospital	1789:1796	arg1	infections					1798:1807	hospital infections	1789:1807	hospital infections	1789:1807	The results show that the coating system exhibits promising behavior which could contribute to prevent hospital infections at early implantation times.
32600688	5	63	theme	nanoparticles	780:792	arg1	composite					794:802	a biopolymer/silica-gentamicin nanoparticles composite	749:802	a biopolymer/silica-gentamicin nanoparticles composite obtained by electrophoretic deposition (EPD) on surgical grade stainless steel plates	749:888	The coating is composed of a biopolymer/silica-gentamicin nanoparticles composite obtained by electrophoretic deposition (EPD) on surgical grade stainless steel plates.
32600688	2	64	theme	common	273:278	arg1	biomaterials					291:302	the most common orthopedic biomaterials	264:302	the most common orthopedic biomaterials in many developing countries	264:331	One of the most common orthopedic biomaterials in many developing countries is surgical grade stainless steel (SS).
32600688	9	65	theme	coatings	1494:1501	arg1	degradation					1458:1468	The degradation	1454:1468	The degradation of the chitosan-gelatin coatings	1454:1501	The degradation of the chitosan-gelatin coatings was evident after 7 days of immersion.
32600688	9	65	theme	coatings	1494:1501	arg1	evident					1507:1513	evident	1507:1513	evident	1507:1513	The degradation of the chitosan-gelatin coatings was evident after 7 days of immersion.
32600688	6	66	theme	simulated	1057:1065	arg1	SBF					1079:1081	SBF	1079:1081	SBF	1079:1081	The coating surface was characterized by microscopic examination, and in vitro performance was evaluated after immersion in phosphate-buffered saline (PBS) solution, simulated body fluid (SBF), and cell culture medium, to analyze coating degradation, antibiotics release, cell attachment (ST-2 stromal cells), and antibacterial (Escherichia coli and Staphylococcus aureus) properties.
32600688	6	66	theme	simulated	1057:1065	arg1	fluid					1072:1076	simulated body fluid	1057:1076	simulated body fluid (SBF)	1057:1082	The coating surface was characterized by microscopic examination, and in vitro performance was evaluated after immersion in phosphate-buffered saline (PBS) solution, simulated body fluid (SBF), and cell culture medium, to analyze coating degradation, antibiotics release, cell attachment (ST-2 stromal cells), and antibacterial (Escherichia coli and Staphylococcus aureus) properties.
32600688	3	67	from	absence	542:548	arg1	media					505:509	physiological media	491:509	physiological media	491:509	However, its use as permanent implant in orthopedic surgery is conditioned due to its limited corrosion resistance in physiological media, lack of osseointegration, and absence of antibacterial effect.
32600688	1	68	theme	fixation	186:193	arg1	elements					195:202	fixation elements	186:202	fixation elements for the stabilization of fractures or as prostheses	186:254	Metals are used in several orthopedic applications as fixation elements for the stabilization of fractures or as prostheses.
32600688	4	69	theme	implants/medical	696:711	arg1	devices					713:719	implants/medical devices	696:719	implants/medical devices	696:719	The aim of this work is to generate a degradable coating with antibacterial properties for stainless steel to be used in implants/medical devices.
32600688	11	70	theme	implantation	1818:1829	arg1	times					1831:1835	early implantation times	1812:1835	early implantation times	1812:1835	The results show that the coating system exhibits promising behavior which could contribute to prevent hospital infections at early implantation times.
32600688	5	71	from	deposition	832:841	arg1	plates					883:888	surgical grade stainless steel plates	852:888	surgical grade stainless steel plates	852:888	The coating is composed of a biopolymer/silica-gentamicin nanoparticles composite obtained by electrophoretic deposition (EPD) on surgical grade stainless steel plates.
32600688	0	72	from	deposition	86:95	arg1	grade					109:113	surgical grade	100:113	surgical grade	100:113	Functional behavior of chitosan/gelatin/silica-gentamicin coatings by electrophoretic deposition on surgical grade stainless steel.
32600688	3	73	from	implant	403:409	arg1	surgery					425:431	orthopedic surgery	414:431	orthopedic surgery	414:431	However, its use as permanent implant in orthopedic surgery is conditioned due to its limited corrosion resistance in physiological media, lack of osseointegration, and absence of antibacterial effect.
32600688	6	74	theme	cell	1089:1092	arg1	medium					1102:1107	cell culture medium	1089:1107	cell culture medium	1089:1107	The coating surface was characterized by microscopic examination, and in vitro performance was evaluated after immersion in phosphate-buffered saline (PBS) solution, simulated body fluid (SBF), and cell culture medium, to analyze coating degradation, antibiotics release, cell attachment (ST-2 stromal cells), and antibacterial (Escherichia coli and Staphylococcus aureus) properties.
32600688	0	75	theme	chitosan/gelatin/silica-gentamicin	23:56	arg1	coatings					58:65	chitosan/gelatin/silica-gentamicin coatings	23:65	chitosan/gelatin/silica-gentamicin coatings	23:65	Functional behavior of chitosan/gelatin/silica-gentamicin coatings by electrophoretic deposition on surgical grade stainless steel.
32600688	8	76	theme	silica-gentamicin	1386:1402	arg1	nanoparticles					1404:1416	silica-gentamicin nanoparticles	1386:1416	silica-gentamicin nanoparticles	1386:1416	Also the distribution of silica-gentamicin nanoparticles was homogeneous on the coated area.
32600688	5	77	theme	electrophoretic	816:830	arg1	EPD					844:846	EPD	844:846	EPD	844:846	The coating is composed of a biopolymer/silica-gentamicin nanoparticles composite obtained by electrophoretic deposition (EPD) on surgical grade stainless steel plates.
32600688	5	77	theme	electrophoretic	816:830	arg1	deposition					832:841	electrophoretic deposition	816:841	electrophoretic deposition (EPD) on surgical grade stainless steel plates	816:888	The coating is composed of a biopolymer/silica-gentamicin nanoparticles composite obtained by electrophoretic deposition (EPD) on surgical grade stainless steel plates.
32600688	3	78	theme	corrosion	467:475	arg1	resistance					477:486	its limited corrosion resistance	455:486	its limited corrosion resistance in physiological media	455:509	However, its use as permanent implant in orthopedic surgery is conditioned due to its limited corrosion resistance in physiological media, lack of osseointegration, and absence of antibacterial effect.
32600688	3	79	theme	orthopedic	414:423	arg1	surgery					425:431	orthopedic surgery	414:431	orthopedic surgery	414:431	However, its use as permanent implant in orthopedic surgery is conditioned due to its limited corrosion resistance in physiological media, lack of osseointegration, and absence of antibacterial effect.
32600688	3	80	from	resistance	477:486	arg1	media					505:509	physiological media	491:509	physiological media	491:509	However, its use as permanent implant in orthopedic surgery is conditioned due to its limited corrosion resistance in physiological media, lack of osseointegration, and absence of antibacterial effect.
32600688	7	81	theme	EPD	1276:1278	arg1	coatings					1280:1287	EPD coatings	1276:1287	EPD coatings	1276:1287	EPD coatings were uniform and covered homogeneously the surface of the SS substrate.
32600688	11	82	theme	promising	1736:1744	arg1	behavior					1746:1753	promising behavior	1736:1753	promising behavior which could contribute to prevent hospital infections at early implantation times	1736:1835	The results show that the coating system exhibits promising behavior which could contribute to prevent hospital infections at early implantation times.
32600688	8	83	theme	coated	1441:1446	arg1	area					1448:1451	the coated area	1437:1451	the coated area	1437:1451	Also the distribution of silica-gentamicin nanoparticles was homogeneous on the coated area.
32600688	6	84	theme	coating	1121:1127	arg1	degradation					1129:1139	coating degradation	1121:1139	coating degradation	1121:1139	The coating surface was characterized by microscopic examination, and in vitro performance was evaluated after immersion in phosphate-buffered saline (PBS) solution, simulated body fluid (SBF), and cell culture medium, to analyze coating degradation, antibiotics release, cell attachment (ST-2 stromal cells), and antibacterial (Escherichia coli and Staphylococcus aureus) properties.
32600688	6	85	theme	culture	1094:1100	arg1	medium					1102:1107	cell culture medium	1089:1107	cell culture medium	1089:1107	The coating surface was characterized by microscopic examination, and in vitro performance was evaluated after immersion in phosphate-buffered saline (PBS) solution, simulated body fluid (SBF), and cell culture medium, to analyze coating degradation, antibiotics release, cell attachment (ST-2 stromal cells), and antibacterial (Escherichia coli and Staphylococcus aureus) properties.
32600688	10	86	theme	antibacterial	1582:1594	arg1	behavior					1596:1603	excellent antibacterial behavior	1572:1603	excellent antibacterial behavior at 24 h, meanwhile the cell proliferation (at 7 days culture) was not inhibited	1572:1683	The gentamicin release led to excellent antibacterial behavior at 24 h, meanwhile the cell proliferation (at 7 days culture) was not inhibited.
34842675	4	0	theme	multilayered	580:591	arg1	scaffolds					593:601	3D multilayered scaffolds	577:601	3D multilayered scaffolds	577:601	Using the extrusion-based nozzle, the nanocomposites inks were printed to obtain 3D multilayered scaffolds.
34842675	5	1	theme	clay	652:655	arg1	type					644:647	each type	639:647	each type of clay	639:655	To observe the behavior induced by each type of clay on alginate-based inks, rheology studies were performed on composite inks.
34842675	9	2	theme	potential	1407:1415	arg1	methods					1431:1437	potential manufacturing methods	1407:1437	potential manufacturing methods for obtaining nanocomposite hydrogels suitable for 3D printing processes	1407:1510	This study provides novel information on potential manufacturing methods for obtaining nanocomposite hydrogels suitable for 3D printing processes, as well as valuable information on the clay type selection for enabling accurate 3D-printed constructs.
34842675	6	3	theme	scaffolds	903:911	arg1	morphology					874:883	the morphology	870:883	the morphology of the 3D-printed scaffolds	870:911	The structure of the nanocomposites samples was examined using Fourier Transform Infrared Spectrometry and X-ray Diffraction (XRD), while the morphology of the 3D-printed scaffolds was evaluated using Electron Microscopy (SEM, TEM) and Micro-Computed Tomography (Micro-CT).
34842675	5	4	theme	alginate-based	660:673	arg1	inks					675:678	alginate-based inks	660:678	alginate-based inks	660:678	To observe the behavior induced by each type of clay on alginate-based inks, rheology studies were performed on composite inks.
34842675	6	5	theme	Infrared	813:820	arg1	XRD					858:860	XRD	858:860	XRD	858:860	The structure of the nanocomposites samples was examined using Fourier Transform Infrared Spectrometry and X-ray Diffraction (XRD), while the morphology of the 3D-printed scaffolds was evaluated using Electron Microscopy (SEM, TEM) and Micro-Computed Tomography (Micro-CT).
34842675	6	5	theme	Infrared	813:820	arg1	Spectrometry					822:833	Infrared Spectrometry	813:833	Infrared Spectrometry	813:833	The structure of the nanocomposites samples was examined using Fourier Transform Infrared Spectrometry and X-ray Diffraction (XRD), while the morphology of the 3D-printed scaffolds was evaluated using Electron Microscopy (SEM, TEM) and Micro-Computed Tomography (Micro-CT).
34842675	10	6	theme	additive	1739:1746	arg1	manufacturing					1748:1760	the additive manufacturing	1735:1760	the additive manufacturing of 3D constructs designed for bone reconstruction therapy	1735:1818	Moreover, this study constitutes the first comprehensive report related to the screening of several natural clays for the additive manufacturing of 3D constructs designed for bone reconstruction therapy.
34842675	10	7	theme	3D	1765:1766	arg1	constructs					1768:1777	3D constructs	1765:1777	3D constructs designed for bone reconstruction therapy	1765:1818	Moreover, this study constitutes the first comprehensive report related to the screening of several natural clays for the additive manufacturing of 3D constructs designed for bone reconstruction therapy.
34842675	7	8	theme	time	1122:1125	arg1	function					1110:1117	function	1110:1117	function of time	1110:1125	The swelling and dissolvability of each composite scaffold in phosfate buffer solution were followed as function of time.
34842675	7	8	theme	time	1122:1125	arg1	dissolvability					1023:1036	dissolvability	1023:1036	dissolvability	1023:1036	The swelling and dissolvability of each composite scaffold in phosfate buffer solution were followed as function of time.
34842675	7	8	theme	time	1122:1125	arg1	swelling					1010:1017	swelling	1010:1017	swelling	1010:1017	The swelling and dissolvability of each composite scaffold in phosfate buffer solution were followed as function of time.
34842675	7	9	theme	phosfate	1068:1075	arg1	solution					1084:1091	phosfate buffer solution	1068:1091	phosfate buffer solution	1068:1091	The swelling and dissolvability of each composite scaffold in phosfate buffer solution were followed as function of time.
34842675	10	10	theme	bone	1792:1795	arg1	therapy					1812:1818	bone reconstruction therapy	1792:1818	bone reconstruction therapy	1792:1818	Moreover, this study constitutes the first comprehensive report related to the screening of several natural clays for the additive manufacturing of 3D constructs designed for bone reconstruction therapy.
34842675	1	11	theme	studied	207:213	arg1	polysaccharides					215:229	the most studied polysaccharides	198:229	the most studied polysaccharides in the field of regenerative medicine	198:267	Biocompatibility, biodegradability, shear tinning behavior, quick gelation and an easy crosslinking process makes alginate one of the most studied polysaccharides in the field of regenerative medicine.
34842675	8	12	theme	alginate	1200:1207	arg1	sample					1209:1214	the alginate sample	1196:1214	the alginate sample containing unmodified clay	1196:1241	Biological studies indicated that the cells grew in the presence of the alginate sample containing unmodified clay, and were able to proliferate and generate calcium deposits in MG-63 cells in the absence of specific signaling molecules.
34842675	10	13	theme	first	1654:1658	arg1	report					1674:1679	the first comprehensive report	1650:1679	the first comprehensive report related to the screening of several natural clays for the additive manufacturing of 3D constructs designed for bone reconstruction therapy	1650:1818	Moreover, this study constitutes the first comprehensive report related to the screening of several natural clays for the additive manufacturing of 3D constructs designed for bone reconstruction therapy.
34842675	7	14	theme	scaffold	1056:1063	arg1	function					1110:1117	function	1110:1117	function of time	1110:1125	The swelling and dissolvability of each composite scaffold in phosfate buffer solution were followed as function of time.
34842675	7	14	theme	scaffold	1056:1063	arg1	dissolvability					1023:1036	dissolvability	1023:1036	dissolvability	1023:1036	The swelling and dissolvability of each composite scaffold in phosfate buffer solution were followed as function of time.
34842675	7	14	theme	scaffold	1056:1063	arg1	swelling					1010:1017	swelling	1010:1017	swelling	1010:1017	The swelling and dissolvability of each composite scaffold in phosfate buffer solution were followed as function of time.
34842675	8	15	contain	containing	1216:1225	arg1	sample					1209:1214	the alginate sample	1196:1214	the alginate sample containing unmodified clay	1196:1241	Biological studies indicated that the cells grew in the presence of the alginate sample containing unmodified clay, and were able to proliferate and generate calcium deposits in MG-63 cells in the absence of specific signaling molecules.
34842675	8	15	contain	containing	1216:1225	arg2	clay					1238:1241	unmodified clay	1227:1241	unmodified clay	1227:1241	Biological studies indicated that the cells grew in the presence of the alginate sample containing unmodified clay, and were able to proliferate and generate calcium deposits in MG-63 cells in the absence of specific signaling molecules.
34842675	1	16	from	polysaccharides	215:229	arg1	field					238:242	the field	234:242	the field of regenerative medicine	234:267	Biocompatibility, biodegradability, shear tinning behavior, quick gelation and an easy crosslinking process makes alginate one of the most studied polysaccharides in the field of regenerative medicine.
34842675	9	17	theme	printing	1493:1500	arg1	processes					1502:1510	3D printing processes	1490:1510	3D printing processes	1490:1510	This study provides novel information on potential manufacturing methods for obtaining nanocomposite hydrogels suitable for 3D printing processes, as well as valuable information on the clay type selection for enabling accurate 3D-printed constructs.
34842675	1	18	theme	gelation	134:141	arg1	process					168:174	Biocompatibility, biodegradability, shear tinning behavior, quick gelation and an easy crosslinking process	68:174	Biocompatibility, biodegradability, shear tinning behavior, quick gelation and an easy crosslinking process	68:174	Biocompatibility, biodegradability, shear tinning behavior, quick gelation and an easy crosslinking process makes alginate one of the most studied polysaccharides in the field of regenerative medicine.
34842675	6	19	theme	Electron	933:940	arg1	SEM					954:956	SEM	954:956	SEM	954:956	The structure of the nanocomposites samples was examined using Fourier Transform Infrared Spectrometry and X-ray Diffraction (XRD), while the morphology of the 3D-printed scaffolds was evaluated using Electron Microscopy (SEM, TEM) and Micro-Computed Tomography (Micro-CT).
34842675	6	19	theme	Electron	933:940	arg1	Microscopy					942:951	Electron Microscopy	933:951	Electron Microscopy (SEM, TEM)	933:962	The structure of the nanocomposites samples was examined using Fourier Transform Infrared Spectrometry and X-ray Diffraction (XRD), while the morphology of the 3D-printed scaffolds was evaluated using Electron Microscopy (SEM, TEM) and Micro-Computed Tomography (Micro-CT).
34842675	9	20	theme	accurate	1585:1592	arg1	constructs					1605:1614	accurate 3D-printed constructs	1585:1614	accurate 3D-printed constructs	1585:1614	This study provides novel information on potential manufacturing methods for obtaining nanocomposite hydrogels suitable for 3D printing processes, as well as valuable information on the clay type selection for enabling accurate 3D-printed constructs.
34842675	8	21	theme	MG-63	1306:1310	arg1	cells					1312:1316	MG-63 cells	1306:1316	MG-63 cells	1306:1316	Biological studies indicated that the cells grew in the presence of the alginate sample containing unmodified clay, and were able to proliferate and generate calcium deposits in MG-63 cells in the absence of specific signaling molecules.
34842675	9	22	theme	valuable	1524:1531	arg1	information					1533:1543	valuable information	1524:1543	valuable information on the clay type selection for enabling accurate 3D-printed constructs	1524:1614	This study provides novel information on potential manufacturing methods for obtaining nanocomposite hydrogels suitable for 3D printing processes, as well as valuable information on the clay type selection for enabling accurate 3D-printed constructs.
34842675	8	23	from	deposits	1294:1301	arg1	cells					1312:1316	MG-63 cells	1306:1316	MG-63 cells	1306:1316	Biological studies indicated that the cells grew in the presence of the alginate sample containing unmodified clay, and were able to proliferate and generate calcium deposits in MG-63 cells in the absence of specific signaling molecules.
34842675	8	23	from	deposits	1294:1301	arg1	absence					1325:1331	the absence	1321:1331	the absence of specific signaling molecules	1321:1363	Biological studies indicated that the cells grew in the presence of the alginate sample containing unmodified clay, and were able to proliferate and generate calcium deposits in MG-63 cells in the absence of specific signaling molecules.
34842675	4	24	theme	extrusion-based	506:520	arg1	nozzle					522:527	the extrusion-based nozzle	502:527	the extrusion-based nozzle	502:527	Using the extrusion-based nozzle, the nanocomposites inks were printed to obtain 3D multilayered scaffolds.
34842675	9	25	theme	clay	1552:1555	arg1	selection					1562:1570	the clay type selection	1548:1570	the clay type selection for enabling accurate 3D-printed constructs	1548:1614	This study provides novel information on potential manufacturing methods for obtaining nanocomposite hydrogels suitable for 3D printing processes, as well as valuable information on the clay type selection for enabling accurate 3D-printed constructs.
34842675	6	26	theme	Micro-Computed	968:981	arg1	Micro-CT					995:1002	Micro-CT	995:1002	Micro-CT	995:1002	The structure of the nanocomposites samples was examined using Fourier Transform Infrared Spectrometry and X-ray Diffraction (XRD), while the morphology of the 3D-printed scaffolds was evaluated using Electron Microscopy (SEM, TEM) and Micro-Computed Tomography (Micro-CT).
34842675	6	26	theme	Micro-Computed	968:981	arg1	Tomography					983:992	Micro-Computed Tomography	968:992	Micro-Computed Tomography (Micro-CT)	968:1003	The structure of the nanocomposites samples was examined using Fourier Transform Infrared Spectrometry and X-ray Diffraction (XRD), while the morphology of the 3D-printed scaffolds was evaluated using Electron Microscopy (SEM, TEM) and Micro-Computed Tomography (Micro-CT).
34842675	8	27	theme	specific	1336:1343	arg1	molecules					1355:1363	specific signaling molecules	1336:1363	specific signaling molecules	1336:1363	Biological studies indicated that the cells grew in the presence of the alginate sample containing unmodified clay, and were able to proliferate and generate calcium deposits in MG-63 cells in the absence of specific signaling molecules.
34842675	10	28	theme	related	1681:1687	arg1	report					1674:1679	the first comprehensive report	1650:1679	the first comprehensive report related to the screening of several natural clays for the additive manufacturing of 3D constructs designed for bone reconstruction therapy	1650:1818	Moreover, this study constitutes the first comprehensive report related to the screening of several natural clays for the additive manufacturing of 3D constructs designed for bone reconstruction therapy.
34842675	8	29	theme	molecules	1355:1363	arg1	absence					1325:1331	the absence	1321:1331	the absence of specific signaling molecules	1321:1363	Biological studies indicated that the cells grew in the presence of the alginate sample containing unmodified clay, and were able to proliferate and generate calcium deposits in MG-63 cells in the absence of specific signaling molecules.
34842675	10	30	theme	comprehensive	1660:1672	arg1	report					1674:1679	the first comprehensive report	1650:1679	the first comprehensive report related to the screening of several natural clays for the additive manufacturing of 3D constructs designed for bone reconstruction therapy	1650:1818	Moreover, this study constitutes the first comprehensive report related to the screening of several natural clays for the additive manufacturing of 3D constructs designed for bone reconstruction therapy.
34842675	0	31	theme	3D	0:1	arg1	Printing					3:10	3D Printing	0:10	3D Printing of Alginate-Natural	0:30	3D Printing of Alginate-Natural Clay Hydrogel-Based Nanocomposites.
34842675	1	32	theme	tinning	110:116	arg1	behavior					118:125	shear tinning behavior	104:125	shear tinning behavior	104:125	Biocompatibility, biodegradability, shear tinning behavior, quick gelation and an easy crosslinking process makes alginate one of the most studied polysaccharides in the field of regenerative medicine.
34842675	9	33	theme	3D-printed	1594:1603	arg1	constructs					1605:1614	accurate 3D-printed constructs	1585:1614	accurate 3D-printed constructs	1585:1614	This study provides novel information on potential manufacturing methods for obtaining nanocomposite hydrogels suitable for 3D printing processes, as well as valuable information on the clay type selection for enabling accurate 3D-printed constructs.
34842675	3	34	theme	several	406:412	arg1	components					449:458	components	449:458	components of 3D-printing, nanocomposite inks	449:493	In this respect, alginate and several types of clay were investigated as components of 3D-printing, nanocomposite inks.
34842675	3	34	theme	several	406:412	arg1	alginate					393:400	alginate	393:400	alginate	393:400	In this respect, alginate and several types of clay were investigated as components of 3D-printing, nanocomposite inks.
34842675	3	34	theme	several	406:412	arg1	types					414:418	several types	406:418	several types of clay	406:426	In this respect, alginate and several types of clay were investigated as components of 3D-printing, nanocomposite inks.
34842675	0	35	dep	Nanocomposites	52:65	arg1	Printing					3:10	3D Printing	0:10	3D Printing of Alginate-Natural	0:30	3D Printing of Alginate-Natural Clay Hydrogel-Based Nanocomposites.
34842675	6	36	theme	nanocomposites	753:766	arg1	samples					768:774	the nanocomposites samples	749:774	the nanocomposites samples	749:774	The structure of the nanocomposites samples was examined using Fourier Transform Infrared Spectrometry and X-ray Diffraction (XRD), while the morphology of the 3D-printed scaffolds was evaluated using Electron Microscopy (SEM, TEM) and Micro-Computed Tomography (Micro-CT).
34842675	3	37	theme	inks	490:493	arg1	components					449:458	components	449:458	components of 3D-printing, nanocomposite inks	449:493	In this respect, alginate and several types of clay were investigated as components of 3D-printing, nanocomposite inks.
34842675	3	37	theme	inks	490:493	arg1	alginate					393:400	alginate	393:400	alginate	393:400	In this respect, alginate and several types of clay were investigated as components of 3D-printing, nanocomposite inks.
34842675	3	37	theme	inks	490:493	arg1	types					414:418	several types	406:418	several types of clay	406:426	In this respect, alginate and several types of clay were investigated as components of 3D-printing, nanocomposite inks.
34842675	9	38	from	information	1392:1402	arg1	selection					1562:1570	the clay type selection	1548:1570	the clay type selection for enabling accurate 3D-printed constructs	1548:1614	This study provides novel information on potential manufacturing methods for obtaining nanocomposite hydrogels suitable for 3D printing processes, as well as valuable information on the clay type selection for enabling accurate 3D-printed constructs.
34842675	9	38	from	information	1392:1402	arg1	methods					1431:1437	potential manufacturing methods	1407:1437	potential manufacturing methods for obtaining nanocomposite hydrogels suitable for 3D printing processes	1407:1510	This study provides novel information on potential manufacturing methods for obtaining nanocomposite hydrogels suitable for 3D printing processes, as well as valuable information on the clay type selection for enabling accurate 3D-printed constructs.
34842675	1	39	theme	biodegradability	86:101	arg1	process					168:174	Biocompatibility, biodegradability, shear tinning behavior, quick gelation and an easy crosslinking process	68:174	Biocompatibility, biodegradability, shear tinning behavior, quick gelation and an easy crosslinking process	68:174	Biocompatibility, biodegradability, shear tinning behavior, quick gelation and an easy crosslinking process makes alginate one of the most studied polysaccharides in the field of regenerative medicine.
34842675	2	40	theme	suitable	337:344	arg1	materials					327:335	tissue-like materials	315:335	tissue-like materials suitable for use in bone regeneration	315:373	The main purpose of this study was to obtain tissue-like materials suitable for use in bone regeneration.
34842675	1	41	theme	crosslinking	155:166	arg1	process					168:174	Biocompatibility, biodegradability, shear tinning behavior, quick gelation and an easy crosslinking process	68:174	Biocompatibility, biodegradability, shear tinning behavior, quick gelation and an easy crosslinking process	68:174	Biocompatibility, biodegradability, shear tinning behavior, quick gelation and an easy crosslinking process makes alginate one of the most studied polysaccharides in the field of regenerative medicine.
34842675	7	42	from	dissolvability	1023:1036	arg1	solution					1084:1091	phosfate buffer solution	1068:1091	phosfate buffer solution	1068:1091	The swelling and dissolvability of each composite scaffold in phosfate buffer solution were followed as function of time.
34842675	9	43	theme	novel	1386:1390	arg1	information					1392:1402	novel information	1386:1402	novel information on potential manufacturing methods for obtaining nanocomposite hydrogels suitable for 3D printing processes	1386:1510	This study provides novel information on potential manufacturing methods for obtaining nanocomposite hydrogels suitable for 3D printing processes, as well as valuable information on the clay type selection for enabling accurate 3D-printed constructs.
34842675	7	44	from	swelling	1010:1017	arg1	solution					1084:1091	phosfate buffer solution	1068:1091	phosfate buffer solution	1068:1091	The swelling and dissolvability of each composite scaffold in phosfate buffer solution were followed as function of time.
34842675	3	45	dep	3D-printing	463:473	arg1	nanocomposite					476:488	nanocomposite	476:488	nanocomposite	476:488	In this respect, alginate and several types of clay were investigated as components of 3D-printing, nanocomposite inks.
34842675	2	46	theme	bone	357:360	arg1	regeneration					362:373	bone regeneration	357:373	bone regeneration	357:373	The main purpose of this study was to obtain tissue-like materials suitable for use in bone regeneration.
34842675	1	47	theme	medicine	260:267	arg1	field					238:242	the field	234:242	the field of regenerative medicine	234:267	Biocompatibility, biodegradability, shear tinning behavior, quick gelation and an easy crosslinking process makes alginate one of the most studied polysaccharides in the field of regenerative medicine.
34842675	9	48	from	information	1533:1543	arg1	selection					1562:1570	the clay type selection	1548:1570	the clay type selection for enabling accurate 3D-printed constructs	1548:1614	This study provides novel information on potential manufacturing methods for obtaining nanocomposite hydrogels suitable for 3D printing processes, as well as valuable information on the clay type selection for enabling accurate 3D-printed constructs.
34842675	9	48	from	information	1533:1543	arg1	methods					1431:1437	potential manufacturing methods	1407:1437	potential manufacturing methods for obtaining nanocomposite hydrogels suitable for 3D printing processes	1407:1510	This study provides novel information on potential manufacturing methods for obtaining nanocomposite hydrogels suitable for 3D printing processes, as well as valuable information on the clay type selection for enabling accurate 3D-printed constructs.
34842675	4	49	theme	3D	577:578	arg1	scaffolds					593:601	3D multilayered scaffolds	577:601	3D multilayered scaffolds	577:601	Using the extrusion-based nozzle, the nanocomposites inks were printed to obtain 3D multilayered scaffolds.
34842675	2	50	theme	study	295:299	arg1	purpose					279:285	The main purpose	270:285	The main purpose of this study	270:299	The main purpose of this study was to obtain tissue-like materials suitable for use in bone regeneration.
34842675	9	51	theme	manufacturing	1417:1429	arg1	methods					1431:1437	potential manufacturing methods	1407:1437	potential manufacturing methods for obtaining nanocomposite hydrogels suitable for 3D printing processes	1407:1510	This study provides novel information on potential manufacturing methods for obtaining nanocomposite hydrogels suitable for 3D printing processes, as well as valuable information on the clay type selection for enabling accurate 3D-printed constructs.
34842675	6	52	theme	3D-printed	892:901	arg1	scaffolds					903:911	the 3D-printed scaffolds	888:911	the 3D-printed scaffolds	888:911	The structure of the nanocomposites samples was examined using Fourier Transform Infrared Spectrometry and X-ray Diffraction (XRD), while the morphology of the 3D-printed scaffolds was evaluated using Electron Microscopy (SEM, TEM) and Micro-Computed Tomography (Micro-CT).
34842675	2	53	from	use	350:352	arg1	regeneration					362:373	bone regeneration	357:373	bone regeneration	357:373	The main purpose of this study was to obtain tissue-like materials suitable for use in bone regeneration.
34842675	10	54	theme	constructs	1768:1777	arg1	manufacturing					1748:1760	the additive manufacturing	1735:1760	the additive manufacturing of 3D constructs designed for bone reconstruction therapy	1735:1818	Moreover, this study constitutes the first comprehensive report related to the screening of several natural clays for the additive manufacturing of 3D constructs designed for bone reconstruction therapy.
34842675	6	55	dep	Fourier	795:801	arg1	XRD					858:860	XRD	858:860	XRD	858:860	The structure of the nanocomposites samples was examined using Fourier Transform Infrared Spectrometry and X-ray Diffraction (XRD), while the morphology of the 3D-printed scaffolds was evaluated using Electron Microscopy (SEM, TEM) and Micro-Computed Tomography (Micro-CT).
34842675	6	55	dep	Fourier	795:801	arg1	Spectrometry					822:833	Infrared Spectrometry	813:833	Infrared Spectrometry	813:833	The structure of the nanocomposites samples was examined using Fourier Transform Infrared Spectrometry and X-ray Diffraction (XRD), while the morphology of the 3D-printed scaffolds was evaluated using Electron Microscopy (SEM, TEM) and Micro-Computed Tomography (Micro-CT).
34842675	6	55	dep	Fourier	795:801	arg1	Diffraction					845:855	X-ray Diffraction	839:855	X-ray Diffraction	839:855	The structure of the nanocomposites samples was examined using Fourier Transform Infrared Spectrometry and X-ray Diffraction (XRD), while the morphology of the 3D-printed scaffolds was evaluated using Electron Microscopy (SEM, TEM) and Micro-Computed Tomography (Micro-CT).
34842675	6	55	dep	Fourier	795:801	arg1	Transform					803:811	Transform	803:811	Transform	803:811	The structure of the nanocomposites samples was examined using Fourier Transform Infrared Spectrometry and X-ray Diffraction (XRD), while the morphology of the 3D-printed scaffolds was evaluated using Electron Microscopy (SEM, TEM) and Micro-Computed Tomography (Micro-CT).
34842675	8	56	theme	unmodified	1227:1236	arg1	clay					1238:1241	unmodified clay	1227:1241	unmodified clay	1227:1241	Biological studies indicated that the cells grew in the presence of the alginate sample containing unmodified clay, and were able to proliferate and generate calcium deposits in MG-63 cells in the absence of specific signaling molecules.
34842675	7	57	theme	buffer	1077:1082	arg1	solution					1084:1091	phosfate buffer solution	1068:1091	phosfate buffer solution	1068:1091	The swelling and dissolvability of each composite scaffold in phosfate buffer solution were followed as function of time.
34842675	2	58	theme	tissue-like	315:325	arg1	materials					327:335	tissue-like materials	315:335	tissue-like materials suitable for use in bone regeneration	315:373	The main purpose of this study was to obtain tissue-like materials suitable for use in bone regeneration.
34842675	9	59	theme	nanocomposite	1453:1465	arg1	hydrogels					1467:1475	nanocomposite hydrogels	1453:1475	nanocomposite hydrogels suitable for 3D printing processes	1453:1510	This study provides novel information on potential manufacturing methods for obtaining nanocomposite hydrogels suitable for 3D printing processes, as well as valuable information on the clay type selection for enabling accurate 3D-printed constructs.
34842675	10	60	theme	reconstruction	1797:1810	arg1	therapy					1812:1818	bone reconstruction therapy	1792:1818	bone reconstruction therapy	1792:1818	Moreover, this study constitutes the first comprehensive report related to the screening of several natural clays for the additive manufacturing of 3D constructs designed for bone reconstruction therapy.
34842675	9	61	theme	suitable	1477:1484	arg1	hydrogels					1467:1475	nanocomposite hydrogels	1453:1475	nanocomposite hydrogels suitable for 3D printing processes	1453:1510	This study provides novel information on potential manufacturing methods for obtaining nanocomposite hydrogels suitable for 3D printing processes, as well as valuable information on the clay type selection for enabling accurate 3D-printed constructs.
34842675	7	62	theme	composite	1046:1054	arg1	scaffold					1056:1063	each composite scaffold	1041:1063	each composite scaffold	1041:1063	The swelling and dissolvability of each composite scaffold in phosfate buffer solution were followed as function of time.
34842675	9	63	theme	3D	1490:1491	arg1	processes					1502:1510	3D printing processes	1490:1510	3D printing processes	1490:1510	This study provides novel information on potential manufacturing methods for obtaining nanocomposite hydrogels suitable for 3D printing processes, as well as valuable information on the clay type selection for enabling accurate 3D-printed constructs.
34842675	6	64	dep	SEM	954:956	arg1	TEM					959:961	TEM	959:961	TEM	959:961	The structure of the nanocomposites samples was examined using Fourier Transform Infrared Spectrometry and X-ray Diffraction (XRD), while the morphology of the 3D-printed scaffolds was evaluated using Electron Microscopy (SEM, TEM) and Micro-Computed Tomography (Micro-CT).
34842675	4	65	theme	nanocomposites	534:547	arg1	inks					549:552	the nanocomposites inks	530:552	the nanocomposites inks	530:552	Using the extrusion-based nozzle, the nanocomposites inks were printed to obtain 3D multilayered scaffolds.
34842675	1	66	theme	quick	128:132	arg1	gelation					134:141	quick gelation	128:141	quick gelation	128:141	Biocompatibility, biodegradability, shear tinning behavior, quick gelation and an easy crosslinking process makes alginate one of the most studied polysaccharides in the field of regenerative medicine.
34842675	5	67	theme	rheology	681:688	arg1	studies					690:696	rheology studies	681:696	rheology studies	681:696	To observe the behavior induced by each type of clay on alginate-based inks, rheology studies were performed on composite inks.
34842675	2	68	theme	main	274:277	arg1	purpose					279:285	The main purpose	270:285	The main purpose of this study	270:299	The main purpose of this study was to obtain tissue-like materials suitable for use in bone regeneration.
34842675	8	69	theme	calcium	1286:1292	arg1	deposits					1294:1301	calcium deposits	1286:1301	calcium deposits in MG-63 cells in the absence of specific signaling molecules	1286:1363	Biological studies indicated that the cells grew in the presence of the alginate sample containing unmodified clay, and were able to proliferate and generate calcium deposits in MG-63 cells in the absence of specific signaling molecules.
34842675	10	70	theme	natural	1717:1723	arg1	clays					1725:1729	several natural clays	1709:1729	several natural clays for the additive manufacturing of 3D constructs designed for bone reconstruction therapy	1709:1818	Moreover, this study constitutes the first comprehensive report related to the screening of several natural clays for the additive manufacturing of 3D constructs designed for bone reconstruction therapy.
34842675	1	71	theme	easy	150:153	arg1	crosslinking					155:166	an easy crosslinking	147:166	an easy crosslinking	147:166	Biocompatibility, biodegradability, shear tinning behavior, quick gelation and an easy crosslinking process makes alginate one of the most studied polysaccharides in the field of regenerative medicine.
34842675	1	72	theme	Biocompatibility	68:83	arg1	process					168:174	Biocompatibility, biodegradability, shear tinning behavior, quick gelation and an easy crosslinking process	68:174	Biocompatibility, biodegradability, shear tinning behavior, quick gelation and an easy crosslinking process	68:174	Biocompatibility, biodegradability, shear tinning behavior, quick gelation and an easy crosslinking process makes alginate one of the most studied polysaccharides in the field of regenerative medicine.
34842675	9	73	theme	type	1557:1560	arg1	selection					1562:1570	the clay type selection	1548:1570	the clay type selection for enabling accurate 3D-printed constructs	1548:1614	This study provides novel information on potential manufacturing methods for obtaining nanocomposite hydrogels suitable for 3D printing processes, as well as valuable information on the clay type selection for enabling accurate 3D-printed constructs.
34842675	1	74	theme	shear	104:108	arg1	behavior					118:125	shear tinning behavior	104:125	shear tinning behavior	104:125	Biocompatibility, biodegradability, shear tinning behavior, quick gelation and an easy crosslinking process makes alginate one of the most studied polysaccharides in the field of regenerative medicine.
34842675	8	75	theme	signaling	1345:1353	arg1	molecules					1355:1363	specific signaling molecules	1336:1363	specific signaling molecules	1336:1363	Biological studies indicated that the cells grew in the presence of the alginate sample containing unmodified clay, and were able to proliferate and generate calcium deposits in MG-63 cells in the absence of specific signaling molecules.
34842675	3	76	theme	clay	423:426	arg1	components					449:458	components	449:458	components of 3D-printing, nanocomposite inks	449:493	In this respect, alginate and several types of clay were investigated as components of 3D-printing, nanocomposite inks.
34842675	3	76	theme	clay	423:426	arg1	alginate					393:400	alginate	393:400	alginate	393:400	In this respect, alginate and several types of clay were investigated as components of 3D-printing, nanocomposite inks.
34842675	3	76	theme	clay	423:426	arg1	types					414:418	several types	406:418	several types of clay	406:426	In this respect, alginate and several types of clay were investigated as components of 3D-printing, nanocomposite inks.
34842675	0	77	theme	Alginate-Natural	15:30	arg1	Printing					3:10	3D Printing	0:10	3D Printing of Alginate-Natural	0:30	3D Printing of Alginate-Natural Clay Hydrogel-Based Nanocomposites.
34842675	1	78	theme	behavior	118:125	arg1	process					168:174	Biocompatibility, biodegradability, shear tinning behavior, quick gelation and an easy crosslinking process	68:174	Biocompatibility, biodegradability, shear tinning behavior, quick gelation and an easy crosslinking process	68:174	Biocompatibility, biodegradability, shear tinning behavior, quick gelation and an easy crosslinking process makes alginate one of the most studied polysaccharides in the field of regenerative medicine.
34842675	8	79	dep	sample	1209:1214	arg1	presence					1184:1191	presence	1184:1191	presence	1184:1191	Biological studies indicated that the cells grew in the presence of the alginate sample containing unmodified clay, and were able to proliferate and generate calcium deposits in MG-63 cells in the absence of specific signaling molecules.
34842675	8	79	dep	sample	1209:1214	arg1	the					1180:1182	the	1180:1182	the	1180:1182	Biological studies indicated that the cells grew in the presence of the alginate sample containing unmodified clay, and were able to proliferate and generate calcium deposits in MG-63 cells in the absence of specific signaling molecules.
34842675	3	80	theme	3D-printing	463:473	arg1	inks					490:493	3D-printing, nanocomposite inks	463:493	3D-printing, nanocomposite inks	463:493	In this respect, alginate and several types of clay were investigated as components of 3D-printing, nanocomposite inks.
34842675	1	81	theme	polysaccharides	215:229	arg1	polysaccharides					215:229	the most studied polysaccharides	198:229	the most studied polysaccharides in the field of regenerative medicine	198:267	Biocompatibility, biodegradability, shear tinning behavior, quick gelation and an easy crosslinking process makes alginate one of the most studied polysaccharides in the field of regenerative medicine.
34842675	1	81	theme	polysaccharides	215:229	arg1	one					191:193	one	191:193	one	191:193	Biocompatibility, biodegradability, shear tinning behavior, quick gelation and an easy crosslinking process makes alginate one of the most studied polysaccharides in the field of regenerative medicine.
34842675	6	82	theme	samples	768:774	arg1	structure					736:744	The structure	732:744	The structure of the nanocomposites samples	732:774	The structure of the nanocomposites samples was examined using Fourier Transform Infrared Spectrometry and X-ray Diffraction (XRD), while the morphology of the 3D-printed scaffolds was evaluated using Electron Microscopy (SEM, TEM) and Micro-Computed Tomography (Micro-CT).
34842675	5	83	theme	composite	716:724	arg1	inks					726:729	composite inks	716:729	composite inks	716:729	To observe the behavior induced by each type of clay on alginate-based inks, rheology studies were performed on composite inks.
34842675	10	84	theme	clays	1725:1729	arg1	screening					1696:1704	the screening	1692:1704	the screening of several natural clays for the additive manufacturing of 3D constructs designed for bone reconstruction therapy	1692:1818	Moreover, this study constitutes the first comprehensive report related to the screening of several natural clays for the additive manufacturing of 3D constructs designed for bone reconstruction therapy.
34842675	10	85	theme	several	1709:1715	arg1	clays					1725:1729	several natural clays	1709:1729	several natural clays for the additive manufacturing of 3D constructs designed for bone reconstruction therapy	1709:1818	Moreover, this study constitutes the first comprehensive report related to the screening of several natural clays for the additive manufacturing of 3D constructs designed for bone reconstruction therapy.
34842675	8	86	theme	Biological	1128:1137	arg1	studies					1139:1145	Biological studies	1128:1145	Biological studies	1128:1145	Biological studies indicated that the cells grew in the presence of the alginate sample containing unmodified clay, and were able to proliferate and generate calcium deposits in MG-63 cells in the absence of specific signaling molecules.
34842675	6	87	theme	X-ray	839:843	arg1	Diffraction					845:855	X-ray Diffraction	839:855	X-ray Diffraction	839:855	The structure of the nanocomposites samples was examined using Fourier Transform Infrared Spectrometry and X-ray Diffraction (XRD), while the morphology of the 3D-printed scaffolds was evaluated using Electron Microscopy (SEM, TEM) and Micro-Computed Tomography (Micro-CT).
34842675	1	88	theme	regenerative	247:258	arg1	medicine					260:267	regenerative medicine	247:267	regenerative medicine	247:267	Biocompatibility, biodegradability, shear tinning behavior, quick gelation and an easy crosslinking process makes alginate one of the most studied polysaccharides in the field of regenerative medicine.
32794528	1	0	theme	chitosan	161:168	arg1	ELCE					180:183	ELCE	180:183	ELCE	180:183	Eugenol-loaded chitosan emulsion (ELCE) has been proved to have an excellent antibacterial property.
32794528	1	0	theme	chitosan	161:168	arg1	emulsion					170:177	Eugenol-loaded chitosan emulsion	146:177	Eugenol-loaded chitosan emulsion (ELCE)	146:184	Eugenol-loaded chitosan emulsion (ELCE) has been proved to have an excellent antibacterial property.
32794528	5	1	theme	heavy	840:844	arg1	chain					846:850	slow myosin heavy chain 2	828:852	slow myosin heavy chain 2	828:852	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	1	theme	heavy	840:844	arg1	chain					821:825	myosin heavy chain	808:825	myosin heavy chain	808:825	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	1	theme	heavy	840:844	arg1	myosin					859:864	myosin	859:864	myosin	859:864	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	1	theme	heavy	840:844	arg1	proteins					908:915	the top 3 significantly high content proteins	871:915	the top 3 significantly high content proteins of ELCE-treated hairtail	871:940	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	2	theme	treated	753:759	arg1	groups					773:778	treated and control groups	753:778	treated and control groups at the endpoint of storage	753:805	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	6	3	theme	microbial	1030:1038	arg1	degradation					1040:1050	microbial degradation	1030:1050	microbial degradation of structural proteins	1030:1073	By preventing microbial degradation of structural proteins, ELCE kept the texture of hairtail, providing customers with high-quality hairtail.
32794528	3	4	theme	hairtail	508:515	arg1	texture					489:495	the texture	485:495	the texture of chilled hairtail	485:515	The method of iTRAQ labeled quantitative proteome analysis was used to clarify the mechanism for the phenomenon that ELCE retained the texture of chilled hairtail at the molecular level.
32794528	2	5	theme	ELCE-treated	277:288	arg1	hairtail					290:297	ELCE-treated hairtail	277:297	ELCE-treated hairtail	277:297	In this study, the texture of ELCE-treated hairtail was found to be better than that of the control group.
32794528	4	6	theme	protein	557:563	arg1	composition					565:575	the protein composition	553:575	the protein composition at the starting point of storage	553:608	Compared to the protein composition at the starting point of storage, for ELCE-treated and control groups, 277 and 187 proteins were selected as differential proteins for ELCE-treated hairtail, respectively.
32794528	4	7	theme	differential	686:697	arg1	proteins					699:706	differential proteins	686:706	differential proteins for ELCE-treated hairtail	686:732	Compared to the protein composition at the starting point of storage, for ELCE-treated and control groups, 277 and 187 proteins were selected as differential proteins for ELCE-treated hairtail, respectively.
32794528	4	7	theme	differential	686:697	arg1	proteins					660:667	277 and 187 proteins	648:667	277 and 187 proteins	648:667	Compared to the protein composition at the starting point of storage, for ELCE-treated and control groups, 277 and 187 proteins were selected as differential proteins for ELCE-treated hairtail, respectively.
32794528	5	8	theme	high	895:898	arg1	chain					846:850	slow myosin heavy chain 2	828:852	slow myosin heavy chain 2	828:852	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	8	theme	high	895:898	arg1	chain					821:825	myosin heavy chain	808:825	myosin heavy chain	808:825	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	8	theme	high	895:898	arg1	myosin					859:864	myosin	859:864	myosin	859:864	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	8	theme	high	895:898	arg1	proteins					908:915	the top 3 significantly high content proteins	871:915	the top 3 significantly high content proteins of ELCE-treated hairtail	871:940	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	4	9	theme	ELCE-treated	712:723	arg1	hairtail					725:732	ELCE-treated hairtail	712:732	ELCE-treated hairtail	712:732	Compared to the protein composition at the starting point of storage, for ELCE-treated and control groups, 277 and 187 proteins were selected as differential proteins for ELCE-treated hairtail, respectively.
32794528	5	10	theme	myosin	808:813	arg1	chain					846:850	slow myosin heavy chain 2	828:852	slow myosin heavy chain 2	828:852	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	10	theme	myosin	808:813	arg1	chain					821:825	myosin heavy chain	808:825	myosin heavy chain	808:825	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	10	theme	myosin	808:813	arg1	myosin					859:864	myosin	859:864	myosin	859:864	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	10	theme	myosin	808:813	arg1	proteins					908:915	the top 3 significantly high content proteins	871:915	the top 3 significantly high content proteins of ELCE-treated hairtail	871:940	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	0	11	theme	mechanism	101:109	arg1	exploration					111:121	mechanism exploration	101:121	mechanism exploration by proteomic analysis	101:143	Eugenol-loaded chitosan emulsion holds the texture of chilled hairtail (Trichiurus lepturus) better: mechanism exploration by proteomic analysis.
32794528	5	12	theme	control	765:771	arg1	groups					773:778	treated and control groups	753:778	treated and control groups at the endpoint of storage	753:805	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	3	13	used	used	417:420	arg2	method					358:363	The method	354:363	The method of iTRAQ labeled quantitative proteome analysis	354:411	The method of iTRAQ labeled quantitative proteome analysis was used to clarify the mechanism for the phenomenon that ELCE retained the texture of chilled hairtail at the molecular level.
32794528	4	14	theme	control	632:638	arg1	groups					640:645	ELCE-treated and control groups	615:645	ELCE-treated and control groups	615:645	Compared to the protein composition at the starting point of storage, for ELCE-treated and control groups, 277 and 187 proteins were selected as differential proteins for ELCE-treated hairtail, respectively.
32794528	4	15	theme	ELCE-treated	615:626	arg1	groups					640:645	ELCE-treated and control groups	615:645	ELCE-treated and control groups	615:645	Compared to the protein composition at the starting point of storage, for ELCE-treated and control groups, 277 and 187 proteins were selected as differential proteins for ELCE-treated hairtail, respectively.
32794528	3	16	theme	chilled	500:506	arg1	hairtail					508:515	chilled hairtail	500:515	chilled hairtail	500:515	The method of iTRAQ labeled quantitative proteome analysis was used to clarify the mechanism for the phenomenon that ELCE retained the texture of chilled hairtail at the molecular level.
32794528	5	17	theme	heavy	815:819	arg1	chain					846:850	slow myosin heavy chain 2	828:852	slow myosin heavy chain 2	828:852	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	17	theme	heavy	815:819	arg1	chain					821:825	myosin heavy chain	808:825	myosin heavy chain	808:825	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	17	theme	heavy	815:819	arg1	myosin					859:864	myosin	859:864	myosin	859:864	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	17	theme	heavy	815:819	arg1	proteins					908:915	the top 3 significantly high content proteins	871:915	the top 3 significantly high content proteins of ELCE-treated hairtail	871:940	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	0	18	theme	chitosan	15:22	arg1	emulsion					24:31	Eugenol-loaded chitosan emulsion	0:31	Eugenol-loaded chitosan emulsion	0:31	Eugenol-loaded chitosan emulsion holds the texture of chilled hairtail (Trichiurus lepturus) better: mechanism exploration by proteomic analysis.
32794528	3	19	dep	ELCE	471:474	arg1	retained					476:483	retained	476:483	ELCE retained the texture of chilled hairtail at the molecular level	471:538	The method of iTRAQ labeled quantitative proteome analysis was used to clarify the mechanism for the phenomenon that ELCE retained the texture of chilled hairtail at the molecular level.
32794528	3	20	theme	proteome	395:402	arg1	analysis					404:411	iTRAQ labeled quantitative proteome analysis	368:411	iTRAQ labeled quantitative proteome analysis	368:411	The method of iTRAQ labeled quantitative proteome analysis was used to clarify the mechanism for the phenomenon that ELCE retained the texture of chilled hairtail at the molecular level.
32794528	0	21	theme	Eugenol-loaded	0:13	arg1	emulsion					24:31	Eugenol-loaded chitosan emulsion	0:31	Eugenol-loaded chitosan emulsion	0:31	Eugenol-loaded chitosan emulsion holds the texture of chilled hairtail (Trichiurus lepturus) better: mechanism exploration by proteomic analysis.
32794528	3	22	theme	molecular	524:532	arg1	level					534:538	the molecular level	520:538	the molecular level	520:538	The method of iTRAQ labeled quantitative proteome analysis was used to clarify the mechanism for the phenomenon that ELCE retained the texture of chilled hairtail at the molecular level.
32794528	3	23	theme	analysis	404:411	arg1	method					358:363	The method	354:363	The method of iTRAQ labeled quantitative proteome analysis	354:411	The method of iTRAQ labeled quantitative proteome analysis was used to clarify the mechanism for the phenomenon that ELCE retained the texture of chilled hairtail at the molecular level.
32794528	2	24	located	found	303:307	arg2	better					315:320	better	315:320	better	315:320	In this study, the texture of ELCE-treated hairtail was found to be better than that of the control group.
32794528	2	24	located	found	303:307	arg2	texture					266:272	the texture	262:272	the texture of ELCE-treated hairtail	262:297	In this study, the texture of ELCE-treated hairtail was found to be better than that of the control group.
32794528	2	24	located	found	303:307	arg1	study					255:259	this study	250:259	this study	250:259	In this study, the texture of ELCE-treated hairtail was found to be better than that of the control group.
32794528	2	25	theme	hairtail	290:297	arg1	texture					266:272	the texture	262:272	the texture of ELCE-treated hairtail	262:297	In this study, the texture of ELCE-treated hairtail was found to be better than that of the control group.
32794528	2	25	theme	hairtail	290:297	arg1	better					315:320	better	315:320	better	315:320	In this study, the texture of ELCE-treated hairtail was found to be better than that of the control group.
32794528	6	26	theme	structural	1055:1064	arg1	proteins					1066:1073	structural proteins	1055:1073	structural proteins	1055:1073	By preventing microbial degradation of structural proteins, ELCE kept the texture of hairtail, providing customers with high-quality hairtail.
32794528	5	27	theme	content	900:906	arg1	chain					846:850	slow myosin heavy chain 2	828:852	slow myosin heavy chain 2	828:852	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	27	theme	content	900:906	arg1	chain					821:825	myosin heavy chain	808:825	myosin heavy chain	808:825	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	27	theme	content	900:906	arg1	myosin					859:864	myosin	859:864	myosin	859:864	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	27	theme	content	900:906	arg1	proteins					908:915	the top 3 significantly high content proteins	871:915	the top 3 significantly high content proteins of ELCE-treated hairtail	871:940	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	28	theme	structure-related	988:1004	arg1	proteins					1006:1013	structure-related proteins	988:1013	structure-related proteins	988:1013	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	0	29	dep	hairtail	62:69	arg1	lepturus					83:90	Trichiurus lepturus	72:90	Trichiurus lepturus	72:90	Eugenol-loaded chitosan emulsion holds the texture of chilled hairtail (Trichiurus lepturus) better: mechanism exploration by proteomic analysis.
32794528	6	30	theme	high-quality	1136:1147	arg1	hairtail					1149:1156	high-quality hairtail	1136:1156	high-quality hairtail	1136:1156	By preventing microbial degradation of structural proteins, ELCE kept the texture of hairtail, providing customers with high-quality hairtail.
32794528	5	31	theme	slow	828:831	arg1	chain					846:850	slow myosin heavy chain 2	828:852	slow myosin heavy chain 2	828:852	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	31	theme	slow	828:831	arg1	chain					821:825	myosin heavy chain	808:825	myosin heavy chain	808:825	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	31	theme	slow	828:831	arg1	myosin					859:864	myosin	859:864	myosin	859:864	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	31	theme	slow	828:831	arg1	proteins					908:915	the top 3 significantly high content proteins	871:915	the top 3 significantly high content proteins of ELCE-treated hairtail	871:940	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	3	32	theme	labeled	374:380	arg1	analysis					404:411	iTRAQ labeled quantitative proteome analysis	368:411	iTRAQ labeled quantitative proteome analysis	368:411	The method of iTRAQ labeled quantitative proteome analysis was used to clarify the mechanism for the phenomenon that ELCE retained the texture of chilled hairtail at the molecular level.
32794528	5	33	theme	proteins	1006:1013	arg1	view					980:983	the view	976:983	the view of structure-related proteins	976:1013	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	4	34	from	point	593:597	arg1	composition					565:575	the protein composition	553:575	the protein composition at the starting point of storage	553:608	Compared to the protein composition at the starting point of storage, for ELCE-treated and control groups, 277 and 187 proteins were selected as differential proteins for ELCE-treated hairtail, respectively.
32794528	5	35	theme	storage	799:805	arg1	endpoint					787:794	the endpoint	783:794	the endpoint of storage	783:805	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	3	36	theme	quantitative	382:393	arg1	analysis					404:411	iTRAQ labeled quantitative proteome analysis	368:411	iTRAQ labeled quantitative proteome analysis	368:411	The method of iTRAQ labeled quantitative proteome analysis was used to clarify the mechanism for the phenomenon that ELCE retained the texture of chilled hairtail at the molecular level.
32794528	0	37	theme	proteomic	126:134	arg1	analysis					136:143	proteomic analysis	126:143	proteomic analysis	126:143	Eugenol-loaded chitosan emulsion holds the texture of chilled hairtail (Trichiurus lepturus) better: mechanism exploration by proteomic analysis.
32794528	4	38	theme	starting	584:591	arg1	point					593:597	the starting point	580:597	the starting point of storage	580:608	Compared to the protein composition at the starting point of storage, for ELCE-treated and control groups, 277 and 187 proteins were selected as differential proteins for ELCE-treated hairtail, respectively.
32794528	4	39	theme	storage	602:608	arg1	point					593:597	the starting point	580:597	the starting point of storage	580:608	Compared to the protein composition at the starting point of storage, for ELCE-treated and control groups, 277 and 187 proteins were selected as differential proteins for ELCE-treated hairtail, respectively.
32794528	0	40	theme	chilled	54:60	arg1	hairtail					62:69	chilled hairtail	54:69	chilled hairtail (Trichiurus lepturus)	54:91	Eugenol-loaded chitosan emulsion holds the texture of chilled hairtail (Trichiurus lepturus) better: mechanism exploration by proteomic analysis.
32794528	5	41	theme	better	956:961	arg1	texture					963:969	a better texture	954:969	a better texture from the view of structure-related proteins	954:1013	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	3	42	theme	iTRAQ	368:372	arg1	analysis					404:411	iTRAQ labeled quantitative proteome analysis	368:411	iTRAQ labeled quantitative proteome analysis	368:411	The method of iTRAQ labeled quantitative proteome analysis was used to clarify the mechanism for the phenomenon that ELCE retained the texture of chilled hairtail at the molecular level.
32794528	1	43	contain	have	205:208	arg2	property					237:244	an excellent antibacterial property	210:244	an excellent antibacterial property	210:244	Eugenol-loaded chitosan emulsion (ELCE) has been proved to have an excellent antibacterial property.
32794528	1	43	contain	have	205:208	arg1	ELCE					180:183	ELCE	180:183	ELCE	180:183	Eugenol-loaded chitosan emulsion (ELCE) has been proved to have an excellent antibacterial property.
32794528	1	43	contain	have	205:208	arg1	emulsion					170:177	Eugenol-loaded chitosan emulsion	146:177	Eugenol-loaded chitosan emulsion (ELCE)	146:184	Eugenol-loaded chitosan emulsion (ELCE) has been proved to have an excellent antibacterial property.
32794528	5	44	from	endpoint	787:794	arg1	groups					773:778	treated and control groups	753:778	treated and control groups at the endpoint of storage	753:805	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	0	45	dep	holds	33:37	arg1	exploration					111:121	mechanism exploration	101:121	mechanism exploration by proteomic analysis	101:143	Eugenol-loaded chitosan emulsion holds the texture of chilled hairtail (Trichiurus lepturus) better: mechanism exploration by proteomic analysis.
32794528	6	46	theme	proteins	1066:1073	arg1	degradation					1040:1050	microbial degradation	1030:1050	microbial degradation of structural proteins	1030:1073	By preventing microbial degradation of structural proteins, ELCE kept the texture of hairtail, providing customers with high-quality hairtail.
32794528	5	47	theme	top	875:877	arg1	chain					846:850	slow myosin heavy chain 2	828:852	slow myosin heavy chain 2	828:852	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	47	theme	top	875:877	arg1	chain					821:825	myosin heavy chain	808:825	myosin heavy chain	808:825	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	47	theme	top	875:877	arg1	myosin					859:864	myosin	859:864	myosin	859:864	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	47	theme	top	875:877	arg1	proteins					908:915	the top 3 significantly high content proteins	871:915	the top 3 significantly high content proteins of ELCE-treated hairtail	871:940	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	6	48	theme	hairtail	1101:1108	arg1	texture					1090:1096	the texture	1086:1096	the texture of hairtail	1086:1108	By preventing microbial degradation of structural proteins, ELCE kept the texture of hairtail, providing customers with high-quality hairtail.
32794528	5	49	theme	ELCE-treated	920:931	arg1	hairtail					933:940	ELCE-treated hairtail	920:940	ELCE-treated hairtail	920:940	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	50	theme	myosin	833:838	arg1	chain					846:850	slow myosin heavy chain 2	828:852	slow myosin heavy chain 2	828:852	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	50	theme	myosin	833:838	arg1	chain					821:825	myosin heavy chain	808:825	myosin heavy chain	808:825	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	50	theme	myosin	833:838	arg1	myosin					859:864	myosin	859:864	myosin	859:864	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	50	theme	myosin	833:838	arg1	proteins					908:915	the top 3 significantly high content proteins	871:915	the top 3 significantly high content proteins of ELCE-treated hairtail	871:940	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	1	51	theme	excellent	213:221	arg1	property					237:244	an excellent antibacterial property	210:244	an excellent antibacterial property	210:244	Eugenol-loaded chitosan emulsion (ELCE) has been proved to have an excellent antibacterial property.
32794528	5	52	from	view	980:983	arg1	texture					963:969	a better texture	954:969	a better texture from the view of structure-related proteins	954:1013	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	53	theme	hairtail	933:940	arg1	chain					846:850	slow myosin heavy chain 2	828:852	slow myosin heavy chain 2	828:852	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	53	theme	hairtail	933:940	arg1	chain					821:825	myosin heavy chain	808:825	myosin heavy chain	808:825	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	53	theme	hairtail	933:940	arg1	myosin					859:864	myosin	859:864	myosin	859:864	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	5	53	theme	hairtail	933:940	arg1	proteins					908:915	the top 3 significantly high content proteins	871:915	the top 3 significantly high content proteins of ELCE-treated hairtail	871:940	For treated and control groups at the endpoint of storage, myosin heavy chain, slow myosin heavy chain 2, and myosin were the top 3 significantly high content proteins of ELCE-treated hairtail, suggesting a better texture from the view of structure-related proteins.
32794528	2	54	theme	control	339:345	arg1	group					347:351	the control group	335:351	the control group	335:351	In this study, the texture of ELCE-treated hairtail was found to be better than that of the control group.
32794528	1	55	theme	antibacterial	223:235	arg1	property					237:244	an excellent antibacterial property	210:244	an excellent antibacterial property	210:244	Eugenol-loaded chitosan emulsion (ELCE) has been proved to have an excellent antibacterial property.
32794528	0	56	theme	hairtail	62:69	arg1	texture					43:49	the texture	39:49	the texture of chilled hairtail (Trichiurus lepturus)	39:91	Eugenol-loaded chitosan emulsion holds the texture of chilled hairtail (Trichiurus lepturus) better: mechanism exploration by proteomic analysis.
32794528	1	57	theme	Eugenol-loaded	146:159	arg1	ELCE					180:183	ELCE	180:183	ELCE	180:183	Eugenol-loaded chitosan emulsion (ELCE) has been proved to have an excellent antibacterial property.
32794528	1	57	theme	Eugenol-loaded	146:159	arg1	emulsion					170:177	Eugenol-loaded chitosan emulsion	146:177	Eugenol-loaded chitosan emulsion (ELCE)	146:184	Eugenol-loaded chitosan emulsion (ELCE) has been proved to have an excellent antibacterial property.
33095697	8	0	theme	lipid	942:946	arg1	profile					948:954	The polar lipid profile	932:954	The polar lipid profile of strain SR4T	932:969	The polar lipid profile of strain SR4T comprised phosphatidylethanolamine, two glycolipids, two aminophospholipids and three unidentified lipids.
33095697	11	1	theme	strain	1210:1215	arg1	SR4T					1217:1220	strain SR4T	1210:1220	strain SR4T	1210:1220	On the basis of the polyphasic evidence, strain SR4T is proposed as representing a novel species of the genus Flammeovirga, for which the name Flammeovirga agarivorans sp.
33095697	4	2	theme	yaeyamensis	500:510	arg1	similarity					548:557	97.99 % 16S rRNA gene similarity	526:557	97.99 % 16S rRNA gene similarity	526:557	It was most closely related to Flammeovirga yaeyamensis NBRC 100898T (97.99 % 16S rRNA gene similarity).
33095697	4	2	theme	yaeyamensis	500:510	arg1	100898T					517:523	Flammeovirga yaeyamensis NBRC 100898T	487:523	Flammeovirga yaeyamensis NBRC 100898T (97.99 % 16S rRNA gene similarity)	487:558	It was most closely related to Flammeovirga yaeyamensis NBRC 100898T (97.99 % 16S rRNA gene similarity).
33095697	5	3	theme	strain	639:644	arg1	SR4T					646:649	strain SR4T	639:649	strain SR4T	639:649	The genome average nucleotide identity and DNA-DNA relatedness values between strain SR4T and its reference strains were less than 74.2 and 16.3 %, respectively.
33095697	5	4	theme	genome	565:570	arg1	identity					591:598	genome average nucleotide identity	565:598	genome average nucleotide identity	565:598	The genome average nucleotide identity and DNA-DNA relatedness values between strain SR4T and its reference strains were less than 74.2 and 16.3 %, respectively.
33095697	2	5	theme	Gram-stain-negative	104:122	arg1	strain					179:184	A Gram-stain-negative, aerobic, gliding, reddish-orange-coloured, rod-shaped strain	102:184	A Gram-stain-negative, aerobic, gliding, reddish-orange-coloured, rod-shaped strain	102:184	A Gram-stain-negative, aerobic, gliding, reddish-orange-coloured, rod-shaped strain, designated SR4T, was isolated from surface seawater sampled at Luhuitou fringing reef (South China Sea).
33095697	5	6	theme	average	572:578	arg1	identity					591:598	genome average nucleotide identity	565:598	genome average nucleotide identity	565:598	The genome average nucleotide identity and DNA-DNA relatedness values between strain SR4T and its reference strains were less than 74.2 and 16.3 %, respectively.
33095697	1	7	theme	marine	53:58	arg1	bacterium					60:68	an agar-digesting marine bacterium	35:68	an agar-digesting marine bacterium	35:68	nov., an agar-digesting marine bacterium isolated from surface seawater.
33095697	1	7	theme	marine	53:58	arg1	nov.					29:32	nov.	29:32	nov.	29:32	nov., an agar-digesting marine bacterium isolated from surface seawater.
33095697	3	8	theme	phylogenomic	342:353	arg1	analysis					355:362	phylogenomic analysis	342:362	phylogenomic analysis of single-copy gene families and whole genome data	342:413	Phylogenetic analyses based on the 16S rRNA gene, phylogenomic analysis of single-copy gene families and whole genome data affiliated it to the genus Flammeovirga.
33095697	5	9	theme	nucleotide	580:589	arg1	identity					591:598	genome average nucleotide identity	565:598	genome average nucleotide identity	565:598	The genome average nucleotide identity and DNA-DNA relatedness values between strain SR4T and its reference strains were less than 74.2 and 16.3 %, respectively.
33095697	2	10	theme	China	280:284	arg1	Sea					286:288	South China Sea	274:288	South China Sea	274:288	A Gram-stain-negative, aerobic, gliding, reddish-orange-coloured, rod-shaped strain, designated SR4T, was isolated from surface seawater sampled at Luhuitou fringing reef (South China Sea).
33095697	2	10	theme	China	280:284	arg1	reef					268:271	Luhuitou fringing reef	250:271	Luhuitou fringing reef (South China Sea)	250:289	A Gram-stain-negative, aerobic, gliding, reddish-orange-coloured, rod-shaped strain, designated SR4T, was isolated from surface seawater sampled at Luhuitou fringing reef (South China Sea).
33095697	10	11	theme	strain	1141:1146	arg1	SR4T					1148:1151	strain SR4T	1141:1151	strain SR4T	1141:1151	The DNA G+C content of strain SR4T was 34.20 mol%.
33095697	10	12	theme	G+C	1126:1128	arg1	content					1130:1136	The DNA G+C content	1118:1136	The DNA G+C content of strain SR4T	1118:1151	The DNA G+C content of strain SR4T was 34.20 mol%.
33095697	10	12	theme	G+C	1126:1128	arg1	%					1166:1166	34.20 mol%	1157:1166	34.20 mol%	1157:1166	The DNA G+C content of strain SR4T was 34.20 mol%.
33095697	3	13	theme	rRNA	331:334	arg1	gene					336:339	the 16S rRNA gene	323:339	the 16S rRNA gene	323:339	Phylogenetic analyses based on the 16S rRNA gene, phylogenomic analysis of single-copy gene families and whole genome data affiliated it to the genus Flammeovirga.
33095697	2	14	theme	South	274:278	arg1	Sea					286:288	South China Sea	274:288	South China Sea	274:288	A Gram-stain-negative, aerobic, gliding, reddish-orange-coloured, rod-shaped strain, designated SR4T, was isolated from surface seawater sampled at Luhuitou fringing reef (South China Sea).
33095697	2	14	theme	South	274:278	arg1	reef					268:271	Luhuitou fringing reef	250:271	Luhuitou fringing reef (South China Sea)	250:289	A Gram-stain-negative, aerobic, gliding, reddish-orange-coloured, rod-shaped strain, designated SR4T, was isolated from surface seawater sampled at Luhuitou fringing reef (South China Sea).
33095697	5	15	theme	DNA-DNA	604:610	arg1	values					624:629	DNA-DNA relatedness values	604:629	DNA-DNA relatedness values	604:629	The genome average nucleotide identity and DNA-DNA relatedness values between strain SR4T and its reference strains were less than 74.2 and 16.3 %, respectively.
33095697	11	16	theme	novel	1252:1256	arg1	species					1258:1264	a novel species	1250:1264	a novel species	1250:1264	On the basis of the polyphasic evidence, strain SR4T is proposed as representing a novel species of the genus Flammeovirga, for which the name Flammeovirga agarivorans sp.
33095697	2	17	attach	isolated	208:215	arg1	seawater					230:237	surface seawater	222:237	surface seawater sampled at Luhuitou fringing reef (South China Sea)	222:289	A Gram-stain-negative, aerobic, gliding, reddish-orange-coloured, rod-shaped strain, designated SR4T, was isolated from surface seawater sampled at Luhuitou fringing reef (South China Sea).
33095697	2	17	attach	isolated	208:215	arg2	strain					179:184	A Gram-stain-negative, aerobic, gliding, reddish-orange-coloured, rod-shaped strain	102:184	A Gram-stain-negative, aerobic, gliding, reddish-orange-coloured, rod-shaped strain	102:184	A Gram-stain-negative, aerobic, gliding, reddish-orange-coloured, rod-shaped strain, designated SR4T, was isolated from surface seawater sampled at Luhuitou fringing reef (South China Sea).
33095697	3	18	theme	single-copy	367:377	arg1	families					384:391	single-copy gene families	367:391	single-copy gene families	367:391	Phylogenetic analyses based on the 16S rRNA gene, phylogenomic analysis of single-copy gene families and whole genome data affiliated it to the genus Flammeovirga.
33095697	6	19	dep	optimum	781:787	arg1	pH					790:791	pH 7.0	790:795	pH 7.0	790:795	Growth occurred at 20-35 °C (optimum, 28 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 1-6 % (w/v) NaCl (optimum, 2-4 %).
33095697	5	20	theme	relatedness	612:622	arg1	values					624:629	DNA-DNA relatedness values	604:629	DNA-DNA relatedness values	604:629	The genome average nucleotide identity and DNA-DNA relatedness values between strain SR4T and its reference strains were less than 74.2 and 16.3 %, respectively.
33095697	1	21	theme	surface	84:90	arg1	seawater					92:99	surface seawater	84:99	surface seawater	84:99	nov., an agar-digesting marine bacterium isolated from surface seawater.
33095697	6	22	dep	optimum	752:758	arg1	28 °C					761:765	28 °C	761:765	28 °C	761:765	Growth occurred at 20-35 °C (optimum, 28 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 1-6 % (w/v) NaCl (optimum, 2-4 %).
33095697	3	23	theme	data	410:413	arg1	analysis					355:362	phylogenomic analysis	342:362	phylogenomic analysis of single-copy gene families and whole genome data	342:413	Phylogenetic analyses based on the 16S rRNA gene, phylogenomic analysis of single-copy gene families and whole genome data affiliated it to the genus Flammeovirga.
33095697	3	24	theme	gene	379:382	arg1	families					384:391	single-copy gene families	367:391	single-copy gene families	367:391	Phylogenetic analyses based on the 16S rRNA gene, phylogenomic analysis of single-copy gene families and whole genome data affiliated it to the genus Flammeovirga.
33095697	4	25	theme	16S	534:536	arg1	similarity					548:557	97.99 % 16S rRNA gene similarity	526:557	97.99 % 16S rRNA gene similarity	526:557	It was most closely related to Flammeovirga yaeyamensis NBRC 100898T (97.99 % 16S rRNA gene similarity).
33095697	4	25	theme	16S	534:536	arg1	100898T					517:523	Flammeovirga yaeyamensis NBRC 100898T	487:523	Flammeovirga yaeyamensis NBRC 100898T (97.99 % 16S rRNA gene similarity)	487:558	It was most closely related to Flammeovirga yaeyamensis NBRC 100898T (97.99 % 16S rRNA gene similarity).
33095697	11	26	dep	evidence	1200:1207	arg1	the					1172:1174	the	1172:1174	the	1172:1174	On the basis of the polyphasic evidence, strain SR4T is proposed as representing a novel species of the genus Flammeovirga, for which the name Flammeovirga agarivorans sp.
33095697	11	26	dep	evidence	1200:1207	arg1	basis					1176:1180	basis	1176:1180	basis	1176:1180	On the basis of the polyphasic evidence, strain SR4T is proposed as representing a novel species of the genus Flammeovirga, for which the name Flammeovirga agarivorans sp.
33095697	13	27	theme	=KCTC	1384:1388	arg1	1A17137T					1402:1409	=KCTC 82075T=MCCC 1A17137T	1384:1409	=KCTC 82075T=MCCC 1A17137T	1384:1409	The type strain is SR4T (=KCTC 82075T=MCCC 1A17137T).
33095697	13	27	theme	=KCTC	1384:1388	arg1	SR4T					1378:1381	SR4T	1378:1381	SR4T (=KCTC 82075T=MCCC 1A17137T)	1378:1410	The type strain is SR4T (=KCTC 82075T=MCCC 1A17137T).
33095697	4	28	theme	%	532:532	arg1	similarity					548:557	97.99 % 16S rRNA gene similarity	526:557	97.99 % 16S rRNA gene similarity	526:557	It was most closely related to Flammeovirga yaeyamensis NBRC 100898T (97.99 % 16S rRNA gene similarity).
33095697	4	28	theme	%	532:532	arg1	100898T					517:523	Flammeovirga yaeyamensis NBRC 100898T	487:523	Flammeovirga yaeyamensis NBRC 100898T (97.99 % 16S rRNA gene similarity)	487:558	It was most closely related to Flammeovirga yaeyamensis NBRC 100898T (97.99 % 16S rRNA gene similarity).
33095697	13	29	theme	82075T=MCCC	1390:1400	arg1	1A17137T					1402:1409	=KCTC 82075T=MCCC 1A17137T	1384:1409	=KCTC 82075T=MCCC 1A17137T	1384:1409	The type strain is SR4T (=KCTC 82075T=MCCC 1A17137T).
33095697	13	29	theme	82075T=MCCC	1390:1400	arg1	SR4T					1378:1381	SR4T	1378:1381	SR4T (=KCTC 82075T=MCCC 1A17137T)	1378:1410	The type strain is SR4T (=KCTC 82075T=MCCC 1A17137T).
33095697	10	30	theme	34.20 mol	1157:1165	arg1	content					1130:1136	The DNA G+C content	1118:1136	The DNA G+C content of strain SR4T	1118:1151	The DNA G+C content of strain SR4T was 34.20 mol%.
33095697	10	30	theme	34.20 mol	1157:1165	arg1	%					1166:1166	34.20 mol%	1157:1166	34.20 mol%	1157:1166	The DNA G+C content of strain SR4T was 34.20 mol%.
33095697	4	31	theme	gene	543:546	arg1	similarity					548:557	97.99 % 16S rRNA gene similarity	526:557	97.99 % 16S rRNA gene similarity	526:557	It was most closely related to Flammeovirga yaeyamensis NBRC 100898T (97.99 % 16S rRNA gene similarity).
33095697	4	31	theme	gene	543:546	arg1	100898T					517:523	Flammeovirga yaeyamensis NBRC 100898T	487:523	Flammeovirga yaeyamensis NBRC 100898T (97.99 % 16S rRNA gene similarity)	487:558	It was most closely related to Flammeovirga yaeyamensis NBRC 100898T (97.99 % 16S rRNA gene similarity).
33095697	9	32	theme	major	1082:1086	arg1	quinone					1100:1106	The major respiratory quinone	1078:1106	The major respiratory quinone	1078:1106	The major respiratory quinone was MK-7.
33095697	9	32	theme	major	1082:1086	arg1	MK-7					1112:1115	MK-7	1112:1115	MK-7	1112:1115	The major respiratory quinone was MK-7.
33095697	11	33	theme	Flammeovirga	1279:1290	arg1	species					1258:1264	a novel species	1250:1264	a novel species	1250:1264	On the basis of the polyphasic evidence, strain SR4T is proposed as representing a novel species of the genus Flammeovirga, for which the name Flammeovirga agarivorans sp.
33095697	11	34	theme	genus	1273:1277	arg1	Flammeovirga					1279:1290	the genus Flammeovirga	1269:1290	the genus Flammeovirga	1269:1290	On the basis of the polyphasic evidence, strain SR4T is proposed as representing a novel species of the genus Flammeovirga, for which the name Flammeovirga agarivorans sp.
33095697	3	35	theme	whole	397:401	arg1	data					410:413	whole genome data	397:413	whole genome data	397:413	Phylogenetic analyses based on the 16S rRNA gene, phylogenomic analysis of single-copy gene families and whole genome data affiliated it to the genus Flammeovirga.
33095697	4	36	theme	rRNA	538:541	arg1	similarity					548:557	97.99 % 16S rRNA gene similarity	526:557	97.99 % 16S rRNA gene similarity	526:557	It was most closely related to Flammeovirga yaeyamensis NBRC 100898T (97.99 % 16S rRNA gene similarity).
33095697	4	36	theme	rRNA	538:541	arg1	100898T					517:523	Flammeovirga yaeyamensis NBRC 100898T	487:523	Flammeovirga yaeyamensis NBRC 100898T (97.99 % 16S rRNA gene similarity)	487:558	It was most closely related to Flammeovirga yaeyamensis NBRC 100898T (97.99 % 16S rRNA gene similarity).
33095697	9	37	theme	respiratory	1088:1098	arg1	quinone					1100:1106	The major respiratory quinone	1078:1106	The major respiratory quinone	1078:1106	The major respiratory quinone was MK-7.
33095697	9	37	theme	respiratory	1088:1098	arg1	MK-7					1112:1115	MK-7	1112:1115	MK-7	1112:1115	The major respiratory quinone was MK-7.
33095697	13	38	theme	type	1363:1366	arg1	strain					1368:1373	The type strain	1359:1373	The type strain	1359:1373	The type strain is SR4T (=KCTC 82075T=MCCC 1A17137T).
33095697	13	38	theme	type	1363:1366	arg1	SR4T					1378:1381	SR4T	1378:1381	SR4T (=KCTC 82075T=MCCC 1A17137T)	1378:1410	The type strain is SR4T (=KCTC 82075T=MCCC 1A17137T).
33095697	10	39	theme	SR4T	1148:1151	arg1	content					1130:1136	The DNA G+C content	1118:1136	The DNA G+C content of strain SR4T	1118:1151	The DNA G+C content of strain SR4T was 34.20 mol%.
33095697	10	39	theme	SR4T	1148:1151	arg1	%					1166:1166	34.20 mol%	1157:1166	34.20 mol%	1157:1166	The DNA G+C content of strain SR4T was 34.20 mol%.
33095697	3	40	theme	genome	403:408	arg1	data					410:413	whole genome data	397:413	whole genome data	397:413	Phylogenetic analyses based on the 16S rRNA gene, phylogenomic analysis of single-copy gene families and whole genome data affiliated it to the genus Flammeovirga.
33095697	8	41	theme	unidentified	1057:1068	arg1	lipids					1070:1075	three unidentified lipids	1051:1075	three unidentified lipids	1051:1075	The polar lipid profile of strain SR4T comprised phosphatidylethanolamine, two glycolipids, two aminophospholipids and three unidentified lipids.
33095697	8	41	theme	unidentified	1057:1068	arg1	phosphatidylethanolamine					981:1004	phosphatidylethanolamine	981:1004	phosphatidylethanolamine	981:1004	The polar lipid profile of strain SR4T comprised phosphatidylethanolamine, two glycolipids, two aminophospholipids and three unidentified lipids.
33095697	6	42	dep	optimum	839:845	arg1	%					852:852	2-4 %	848:852	2-4 %	848:852	Growth occurred at 20-35 °C (optimum, 28 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 1-6 % (w/v) NaCl (optimum, 2-4 %).
33095697	3	43	theme	genus	436:440	arg1	Flammeovirga					442:453	the genus Flammeovirga	432:453	the genus Flammeovirga	432:453	Phylogenetic analyses based on the 16S rRNA gene, phylogenomic analysis of single-copy gene families and whole genome data affiliated it to the genus Flammeovirga.
33095697	3	44	theme	families	384:391	arg1	analysis					355:362	phylogenomic analysis	342:362	phylogenomic analysis of single-copy gene families and whole genome data	342:413	Phylogenetic analyses based on the 16S rRNA gene, phylogenomic analysis of single-copy gene families and whole genome data affiliated it to the genus Flammeovirga.
33095697	2	45	dep	Gram-stain-negative	104:122	arg1	aerobic					125:131	aerobic	125:131	aerobic	125:131	A Gram-stain-negative, aerobic, gliding, reddish-orange-coloured, rod-shaped strain, designated SR4T, was isolated from surface seawater sampled at Luhuitou fringing reef (South China Sea).
33095697	2	45	dep	Gram-stain-negative	104:122	arg1	rod-shaped					168:177	rod-shaped	168:177	rod-shaped	168:177	A Gram-stain-negative, aerobic, gliding, reddish-orange-coloured, rod-shaped strain, designated SR4T, was isolated from surface seawater sampled at Luhuitou fringing reef (South China Sea).
33095697	2	45	dep	Gram-stain-negative	104:122	arg1	reddish-orange-coloured					143:165	reddish-orange-coloured	143:165	reddish-orange-coloured	143:165	A Gram-stain-negative, aerobic, gliding, reddish-orange-coloured, rod-shaped strain, designated SR4T, was isolated from surface seawater sampled at Luhuitou fringing reef (South China Sea).
33095697	2	45	dep	Gram-stain-negative	104:122	arg1	gliding					134:140	gliding	134:140	gliding	134:140	A Gram-stain-negative, aerobic, gliding, reddish-orange-coloured, rod-shaped strain, designated SR4T, was isolated from surface seawater sampled at Luhuitou fringing reef (South China Sea).
33095697	3	46	theme	Phylogenetic	292:303	arg1	analyses					305:312	Phylogenetic analyses	292:312	Phylogenetic analyses based on the 16S rRNA gene	292:339	Phylogenetic analyses based on the 16S rRNA gene, phylogenomic analysis of single-copy gene families and whole genome data affiliated it to the genus Flammeovirga.
33095697	8	47	theme	strain	959:964	arg1	SR4T					966:969	strain SR4T	959:969	strain SR4T	959:969	The polar lipid profile of strain SR4T comprised phosphatidylethanolamine, two glycolipids, two aminophospholipids and three unidentified lipids.
33095697	2	48	theme	surface	222:228	arg1	seawater					230:237	surface seawater	222:237	surface seawater sampled at Luhuitou fringing reef (South China Sea)	222:289	A Gram-stain-negative, aerobic, gliding, reddish-orange-coloured, rod-shaped strain, designated SR4T, was isolated from surface seawater sampled at Luhuitou fringing reef (South China Sea).
33095697	8	49	theme	polar	936:940	arg1	profile					948:954	The polar lipid profile	932:954	The polar lipid profile of strain SR4T	932:969	The polar lipid profile of strain SR4T comprised phosphatidylethanolamine, two glycolipids, two aminophospholipids and three unidentified lipids.
33095697	4	50	theme	Flammeovirga	487:498	arg1	similarity					548:557	97.99 % 16S rRNA gene similarity	526:557	97.99 % 16S rRNA gene similarity	526:557	It was most closely related to Flammeovirga yaeyamensis NBRC 100898T (97.99 % 16S rRNA gene similarity).
33095697	4	50	theme	Flammeovirga	487:498	arg1	100898T					517:523	Flammeovirga yaeyamensis NBRC 100898T	487:523	Flammeovirga yaeyamensis NBRC 100898T (97.99 % 16S rRNA gene similarity)	487:558	It was most closely related to Flammeovirga yaeyamensis NBRC 100898T (97.99 % 16S rRNA gene similarity).
33095697	4	51	theme	97.99 	526:531	arg1	%					532:532	%	532:532	%	532:532	It was most closely related to Flammeovirga yaeyamensis NBRC 100898T (97.99 % 16S rRNA gene similarity).
33095697	5	52	theme	reference	659:667	arg1	strains					669:675	its reference strains	655:675	its reference strains	655:675	The genome average nucleotide identity and DNA-DNA relatedness values between strain SR4T and its reference strains were less than 74.2 and 16.3 %, respectively.
33095697	6	53	theme	%	825:825	arg1	optimum					839:845	optimum	839:845	optimum	839:845	Growth occurred at 20-35 °C (optimum, 28 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 1-6 % (w/v) NaCl (optimum, 2-4 %).
33095697	6	53	theme	%	825:825	arg1	NaCl					833:836	1-6 % (w/v) NaCl	821:836	1-6 % (w/v) NaCl (optimum, 2-4 %)	821:853	Growth occurred at 20-35 °C (optimum, 28 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 1-6 % (w/v) NaCl (optimum, 2-4 %).
33095697	11	54	theme	name	1307:1310	arg1	Flammeovirga					1312:1323	the name Flammeovirga	1303:1323	the name Flammeovirga	1303:1323	On the basis of the polyphasic evidence, strain SR4T is proposed as representing a novel species of the genus Flammeovirga, for which the name Flammeovirga agarivorans sp.
33095697	5	55	dep	identity	591:598	arg1	The					561:563	The	561:563	The	561:563	The genome average nucleotide identity and DNA-DNA relatedness values between strain SR4T and its reference strains were less than 74.2 and 16.3 %, respectively.
33095697	2	56	theme	fringing	259:266	arg1	Sea					286:288	South China Sea	274:288	South China Sea	274:288	A Gram-stain-negative, aerobic, gliding, reddish-orange-coloured, rod-shaped strain, designated SR4T, was isolated from surface seawater sampled at Luhuitou fringing reef (South China Sea).
33095697	2	56	theme	fringing	259:266	arg1	reef					268:271	Luhuitou fringing reef	250:271	Luhuitou fringing reef (South China Sea)	250:289	A Gram-stain-negative, aerobic, gliding, reddish-orange-coloured, rod-shaped strain, designated SR4T, was isolated from surface seawater sampled at Luhuitou fringing reef (South China Sea).
33095697	7	57	theme	dominant	860:867	arg1	C16 					886:889	C16 	886:889	C16 	886:889	The dominant fatty acids were C16 : 0, iso-C15 : 0 and C20 : 4 ω6,9,12,15c.
33095697	7	57	theme	dominant	860:867	arg1	acids					875:879	The dominant fatty acids	856:879	The dominant fatty acids	856:879	The dominant fatty acids were C16 : 0, iso-C15 : 0 and C20 : 4 ω6,9,12,15c.
33095697	8	58	theme	SR4T	966:969	arg1	profile					948:954	The polar lipid profile	932:954	The polar lipid profile of strain SR4T	932:969	The polar lipid profile of strain SR4T comprised phosphatidylethanolamine, two glycolipids, two aminophospholipids and three unidentified lipids.
33095697	2	59	theme	Luhuitou	250:257	arg1	Sea					286:288	South China Sea	274:288	South China Sea	274:288	A Gram-stain-negative, aerobic, gliding, reddish-orange-coloured, rod-shaped strain, designated SR4T, was isolated from surface seawater sampled at Luhuitou fringing reef (South China Sea).
33095697	2	59	theme	Luhuitou	250:257	arg1	reef					268:271	Luhuitou fringing reef	250:271	Luhuitou fringing reef (South China Sea)	250:289	A Gram-stain-negative, aerobic, gliding, reddish-orange-coloured, rod-shaped strain, designated SR4T, was isolated from surface seawater sampled at Luhuitou fringing reef (South China Sea).
33095697	7	60	theme	fatty	869:873	arg1	C16 					886:889	C16 	886:889	C16 	886:889	The dominant fatty acids were C16 : 0, iso-C15 : 0 and C20 : 4 ω6,9,12,15c.
33095697	7	60	theme	fatty	869:873	arg1	acids					875:879	The dominant fatty acids	856:879	The dominant fatty acids	856:879	The dominant fatty acids were C16 : 0, iso-C15 : 0 and C20 : 4 ω6,9,12,15c.
33095697	11	61	theme	polyphasic	1189:1198	arg1	evidence					1200:1207	the polyphasic evidence	1185:1207	the polyphasic evidence	1185:1207	On the basis of the polyphasic evidence, strain SR4T is proposed as representing a novel species of the genus Flammeovirga, for which the name Flammeovirga agarivorans sp.
33095697	6	62	theme	1-6 	821:824	arg1	optimum					839:845	optimum	839:845	optimum	839:845	Growth occurred at 20-35 °C (optimum, 28 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 1-6 % (w/v) NaCl (optimum, 2-4 %).
33095697	6	62	theme	1-6 	821:824	arg1	NaCl					833:836	1-6 % (w/v) NaCl	821:836	1-6 % (w/v) NaCl (optimum, 2-4 %)	821:853	Growth occurred at 20-35 °C (optimum, 28 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 1-6 % (w/v) NaCl (optimum, 2-4 %).
33095697	3	63	theme	16S	327:329	arg1	gene					336:339	the 16S rRNA gene	323:339	the 16S rRNA gene	323:339	Phylogenetic analyses based on the 16S rRNA gene, phylogenomic analysis of single-copy gene families and whole genome data affiliated it to the genus Flammeovirga.
33095697	6	64	theme	w/v	828:830	arg1	optimum					839:845	optimum	839:845	optimum	839:845	Growth occurred at 20-35 °C (optimum, 28 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 1-6 % (w/v) NaCl (optimum, 2-4 %).
33095697	6	64	theme	w/v	828:830	arg1	NaCl					833:836	1-6 % (w/v) NaCl	821:836	1-6 % (w/v) NaCl (optimum, 2-4 %)	821:853	Growth occurred at 20-35 °C (optimum, 28 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 1-6 % (w/v) NaCl (optimum, 2-4 %).
33095697	1	65	theme	agar-digesting	38:51	arg1	bacterium					60:68	an agar-digesting marine bacterium	35:68	an agar-digesting marine bacterium	35:68	nov., an agar-digesting marine bacterium isolated from surface seawater.
33095697	1	65	theme	agar-digesting	38:51	arg1	nov.					29:32	nov.	29:32	nov.	29:32	nov., an agar-digesting marine bacterium isolated from surface seawater.
33095697	6	66	theme	NaCl	833:836	arg1	presence					809:816	the presence	805:816	the presence of 1-6 % (w/v) NaCl (optimum, 2-4 %)	805:853	Growth occurred at 20-35 °C (optimum, 28 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 1-6 % (w/v) NaCl (optimum, 2-4 %).
33095697	10	67	theme	DNA	1122:1124	arg1	content					1130:1136	The DNA G+C content	1118:1136	The DNA G+C content of strain SR4T	1118:1151	The DNA G+C content of strain SR4T was 34.20 mol%.
33095697	10	67	theme	DNA	1122:1124	arg1	%					1166:1166	34.20 mol%	1157:1166	34.20 mol%	1157:1166	The DNA G+C content of strain SR4T was 34.20 mol%.
33095697	4	68	theme	NBRC	512:515	arg1	similarity					548:557	97.99 % 16S rRNA gene similarity	526:557	97.99 % 16S rRNA gene similarity	526:557	It was most closely related to Flammeovirga yaeyamensis NBRC 100898T (97.99 % 16S rRNA gene similarity).
33095697	4	68	theme	NBRC	512:515	arg1	100898T					517:523	Flammeovirga yaeyamensis NBRC 100898T	487:523	Flammeovirga yaeyamensis NBRC 100898T (97.99 % 16S rRNA gene similarity)	487:558	It was most closely related to Flammeovirga yaeyamensis NBRC 100898T (97.99 % 16S rRNA gene similarity).
33095133	9	0	theme	MD130-1T	800:807	arg1	phosphatidylglycerol					814:833	phosphatidylglycerol	814:833	phosphatidylglycerol	814:833	The polar lipids of strain MD130-1T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and triglycosyl diether-2.
33095133	9	0	theme	MD130-1T	800:807	arg1	lipids					783:788	The polar lipids	773:788	The polar lipids of strain MD130-1T	773:807	The polar lipids of strain MD130-1T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and triglycosyl diether-2.
33095133	5	1	theme	Strain	294:299	arg1	MD130-1T					301:308	Strain MD130-1T	294:308	Strain MD130-1T	294:308	Strain MD130-1T was able to grow at 1.5-4.6 M NaCl (optimum, 3.6 M) at pH 6.0-8.0 (optimum, pH 7.0) and at 25-50 °C (optimum, 40 °C).
33095133	7	2	theme	JCM	617:619	arg1	8911T					621:625	Haloarcula hispanica JCM 8911T	596:625	Haloarcula hispanica JCM 8911T	596:625	The orthologous 16S rRNA gene sequence showed the highest similarity (99.4 %) to those of Haloarcula japonica JCM 7785T and Haloarcula hispanica JCM 8911T.
33095133	6	3	theme	62.1 mol	452:459	arg1	content					440:446	The DNA G+C content	428:446	The DNA G+C content	428:446	The DNA G+C content was 62.1 mol% (genome).
33095133	6	3	theme	62.1 mol	452:459	arg1	%					460:460	62.1 mol%	452:460	62.1 mol% (genome)	452:469	The DNA G+C content was 62.1 mol% (genome).
33095133	6	3	theme	62.1 mol	452:459	arg1	genome					463:468	genome	463:468	genome	463:468	The DNA G+C content was 62.1 mol% (genome).
33095133	2	4	theme	archaeal	135:142	arg1	strain					144:149	A mannan-degrading halophilic archaeal strain	105:149	A mannan-degrading halophilic archaeal strain	105:149	A mannan-degrading halophilic archaeal strain, MD130-1T, was isolated from a commercial salt sample.
33095133	2	4	theme	archaeal	135:142	arg1	MD130-1T					152:159	MD130-1T	152:159	MD130-1T	152:159	A mannan-degrading halophilic archaeal strain, MD130-1T, was isolated from a commercial salt sample.
33095133	10	5	theme	phylogenetic	946:957	arg1	analyses					959:966	phenotypic and phylogenetic analyses	931:966	phenotypic and phylogenetic analyses	931:966	Based on the results of phenotypic and phylogenetic analyses, the strain represents a new species of the genus Haloarcula, for which the name Haloarcula mannanilytica sp.
33095133	10	6	theme	phenotypic	931:940	arg1	analyses					959:966	phenotypic and phylogenetic analyses	931:966	phenotypic and phylogenetic analyses	931:966	Based on the results of phenotypic and phylogenetic analyses, the strain represents a new species of the genus Haloarcula, for which the name Haloarcula mannanilytica sp.
33095133	2	7	theme	halophilic	124:133	arg1	strain					144:149	A mannan-degrading halophilic archaeal strain	105:149	A mannan-degrading halophilic archaeal strain	105:149	A mannan-degrading halophilic archaeal strain, MD130-1T, was isolated from a commercial salt sample.
33095133	2	7	theme	halophilic	124:133	arg1	MD130-1T					152:159	MD130-1T	152:159	MD130-1T	152:159	A mannan-degrading halophilic archaeal strain, MD130-1T, was isolated from a commercial salt sample.
33095133	7	8	theme	gene	497:500	arg1	sequence					502:509	The orthologous 16S rRNA gene sequence	472:509	The orthologous 16S rRNA gene sequence	472:509	The orthologous 16S rRNA gene sequence showed the highest similarity (99.4 %) to those of Haloarcula japonica JCM 7785T and Haloarcula hispanica JCM 8911T.
33095133	7	9	theme	Haloarcula	596:605	arg1	8911T					621:625	Haloarcula hispanica JCM 8911T	596:625	Haloarcula hispanica JCM 8911T	596:625	The orthologous 16S rRNA gene sequence showed the highest similarity (99.4 %) to those of Haloarcula japonica JCM 7785T and Haloarcula hispanica JCM 8911T.
33095133	7	10	theme	highest	522:528	arg1	similarity					530:539	the highest similarity	518:539	the highest similarity (99.4 %) to those of Haloarcula japonica JCM 7785T and Haloarcula hispanica JCM 8911T	518:625	The orthologous 16S rRNA gene sequence showed the highest similarity (99.4 %) to those of Haloarcula japonica JCM 7785T and Haloarcula hispanica JCM 8911T.
33095133	7	10	theme	highest	522:528	arg1	%					547:547	99.4 %	542:547	99.4 %	542:547	The orthologous 16S rRNA gene sequence showed the highest similarity (99.4 %) to those of Haloarcula japonica JCM 7785T and Haloarcula hispanica JCM 8911T.
33095133	8	11	from	%	725:725	arg1	ANIb					730:733	ANIb	730:733	ANIb	730:733	The values of genome relatedness between strain MD130-1T and Haloarcula species were 84.33-85.96 % in ANIb and 30.4-32.9 % using GGDC formula 2.
33095133	8	12	theme	GGDC	757:760	arg1	formula					762:768	GGDC formula 2	757:770	GGDC formula 2	757:770	The values of genome relatedness between strain MD130-1T and Haloarcula species were 84.33-85.96 % in ANIb and 30.4-32.9 % using GGDC formula 2.
33095133	7	13	theme	hispanica	607:615	arg1	8911T					621:625	Haloarcula hispanica JCM 8911T	596:625	Haloarcula hispanica JCM 8911T	596:625	The orthologous 16S rRNA gene sequence showed the highest similarity (99.4 %) to those of Haloarcula japonica JCM 7785T and Haloarcula hispanica JCM 8911T.
33095133	6	14	theme	G+C	436:438	arg1	content					440:446	The DNA G+C content	428:446	The DNA G+C content	428:446	The DNA G+C content was 62.1 mol% (genome).
33095133	6	14	theme	G+C	436:438	arg1	%					460:460	62.1 mol%	452:460	62.1 mol% (genome)	452:469	The DNA G+C content was 62.1 mol% (genome).
33095133	7	15	theme	16S	488:490	arg1	gene					497:500	The orthologous 16S rRNA gene	472:500	The orthologous 16S rRNA gene sequence	472:509	The orthologous 16S rRNA gene sequence showed the highest similarity (99.4 %) to those of Haloarcula japonica JCM 7785T and Haloarcula hispanica JCM 8911T.
33095133	6	16	theme	DNA	432:434	arg1	content					440:446	The DNA G+C content	428:446	The DNA G+C content	428:446	The DNA G+C content was 62.1 mol% (genome).
33095133	6	16	theme	DNA	432:434	arg1	%					460:460	62.1 mol%	452:460	62.1 mol% (genome)	452:469	The DNA G+C content was 62.1 mol% (genome).
33095133	12	17	attach	isolated	1149:1156	arg2	4287T					1142:1146	=JCM 33835T=KCTC 4287T	1125:1146	=JCM 33835T=KCTC 4287T	1125:1146	The type strain is MD130-1T (=JCM 33835T=KCTC 4287T) isolated from commercial salt made in Ishikawa prefecture, Japan.
33095133	12	17	attach	isolated	1149:1156	arg2	MD130-1T					1115:1122	MD130-1T	1115:1122	MD130-1T (=JCM 33835T=KCTC 4287T) isolated from commercial salt made in Ishikawa prefecture, Japan	1115:1212	The type strain is MD130-1T (=JCM 33835T=KCTC 4287T) isolated from commercial salt made in Ishikawa prefecture, Japan.
33095133	12	17	attach	isolated	1149:1156	arg2	strain					1105:1110	The type strain	1096:1110	The type strain	1096:1110	The type strain is MD130-1T (=JCM 33835T=KCTC 4287T) isolated from commercial salt made in Ishikawa prefecture, Japan.
33095133	12	17	attach	isolated	1149:1156	arg1	salt					1174:1177	commercial salt	1163:1177	commercial salt made in Ishikawa prefecture, Japan	1163:1212	The type strain is MD130-1T (=JCM 33835T=KCTC 4287T) isolated from commercial salt made in Ishikawa prefecture, Japan.
33095133	1	18	theme	commercial	88:97	arg1	salt					99:102	commercial salt	88:102	commercial salt	88:102	nov., a galactomannan-degrading haloarchaeon isolated from commercial salt.
33095133	9	19	theme	phosphatidylglycerol	836:855	arg1	ester					874:878	phosphatidylglycerol phosphate methyl ester	836:878	phosphatidylglycerol phosphate methyl ester	836:878	The polar lipids of strain MD130-1T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and triglycosyl diether-2.
33095133	0	20	theme	mannanilytica	11:23	arg1	sp					25:26	Haloarcula mannanilytica sp	0:26	Haloarcula mannanilytica sp.	0:27	Haloarcula mannanilytica sp.
33095133	10	21	theme	new	993:995	arg1	species					997:1003	a new species	991:1003	a new species	991:1003	Based on the results of phenotypic and phylogenetic analyses, the strain represents a new species of the genus Haloarcula, for which the name Haloarcula mannanilytica sp.
33095133	10	22	theme	Haloarcula	1049:1058	arg1	sp					1074:1075	the name Haloarcula mannanilytica sp	1040:1075	the name Haloarcula mannanilytica sp	1040:1075	Based on the results of phenotypic and phylogenetic analyses, the strain represents a new species of the genus Haloarcula, for which the name Haloarcula mannanilytica sp.
33095133	8	23	from	%	749:749	arg1	ANIb					730:733	ANIb	730:733	ANIb	730:733	The values of genome relatedness between strain MD130-1T and Haloarcula species were 84.33-85.96 % in ANIb and 30.4-32.9 % using GGDC formula 2.
33095133	0	24	theme	Haloarcula	0:9	arg1	sp					25:26	Haloarcula mannanilytica sp	0:26	Haloarcula mannanilytica sp.	0:27	Haloarcula mannanilytica sp.
33095133	5	25	theme	1.5-4.6	330:336	arg1	M					338:338	M	338:338	M	338:338	Strain MD130-1T was able to grow at 1.5-4.6 M NaCl (optimum, 3.6 M) at pH 6.0-8.0 (optimum, pH 7.0) and at 25-50 °C (optimum, 40 °C).
33095133	7	26	theme	orthologous	476:486	arg1	gene					497:500	The orthologous 16S rRNA gene	472:500	The orthologous 16S rRNA gene sequence	472:509	The orthologous 16S rRNA gene sequence showed the highest similarity (99.4 %) to those of Haloarcula japonica JCM 7785T and Haloarcula hispanica JCM 8911T.
33095133	5	27	theme	M	338:338	arg1	optimum					346:352	optimum	346:352	optimum	346:352	Strain MD130-1T was able to grow at 1.5-4.6 M NaCl (optimum, 3.6 M) at pH 6.0-8.0 (optimum, pH 7.0) and at 25-50 °C (optimum, 40 °C).
33095133	5	27	theme	M	338:338	arg1	NaCl					340:343	1.5-4.6 M NaCl	330:343	1.5-4.6 M NaCl (optimum, 3.6 M)	330:360	Strain MD130-1T was able to grow at 1.5-4.6 M NaCl (optimum, 3.6 M) at pH 6.0-8.0 (optimum, pH 7.0) and at 25-50 °C (optimum, 40 °C).
33095133	5	28	dep	optimum	377:383	arg1	pH					386:387	pH 7.0	386:391	pH 7.0	386:391	Strain MD130-1T was able to grow at 1.5-4.6 M NaCl (optimum, 3.6 M) at pH 6.0-8.0 (optimum, pH 7.0) and at 25-50 °C (optimum, 40 °C).
33095133	9	29	theme	triglycosyl	884:894	arg1	diether-2					896:904	triglycosyl diether-2	884:904	triglycosyl diether-2	884:904	The polar lipids of strain MD130-1T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and triglycosyl diether-2.
33095133	10	30	theme	Haloarcula	1018:1027	arg1	species					997:1003	a new species	991:1003	a new species	991:1003	Based on the results of phenotypic and phylogenetic analyses, the strain represents a new species of the genus Haloarcula, for which the name Haloarcula mannanilytica sp.
33095133	2	31	theme	salt	193:196	arg1	sample					198:203	a commercial salt sample	180:203	a commercial salt sample	180:203	A mannan-degrading halophilic archaeal strain, MD130-1T, was isolated from a commercial salt sample.
33095133	10	32	theme	genus	1012:1016	arg1	Haloarcula					1018:1027	the genus Haloarcula	1008:1027	the genus Haloarcula	1008:1027	Based on the results of phenotypic and phylogenetic analyses, the strain represents a new species of the genus Haloarcula, for which the name Haloarcula mannanilytica sp.
33095133	2	33	theme	commercial	182:191	arg1	sample					198:203	a commercial salt sample	180:203	a commercial salt sample	180:203	A mannan-degrading halophilic archaeal strain, MD130-1T, was isolated from a commercial salt sample.
33095133	12	34	theme	commercial	1163:1172	arg1	salt					1174:1177	commercial salt	1163:1177	commercial salt made in Ishikawa prefecture, Japan	1163:1212	The type strain is MD130-1T (=JCM 33835T=KCTC 4287T) isolated from commercial salt made in Ishikawa prefecture, Japan.
33095133	2	35	theme	mannan-degrading	107:122	arg1	strain					144:149	A mannan-degrading halophilic archaeal strain	105:149	A mannan-degrading halophilic archaeal strain	105:149	A mannan-degrading halophilic archaeal strain, MD130-1T, was isolated from a commercial salt sample.
33095133	2	35	theme	mannan-degrading	107:122	arg1	MD130-1T					152:159	MD130-1T	152:159	MD130-1T	152:159	A mannan-degrading halophilic archaeal strain, MD130-1T, was isolated from a commercial salt sample.
33095133	7	36	theme	japonica	573:580	arg1	7785T					586:590	Haloarcula japonica JCM 7785T	562:590	Haloarcula japonica JCM 7785T	562:590	The orthologous 16S rRNA gene sequence showed the highest similarity (99.4 %) to those of Haloarcula japonica JCM 7785T and Haloarcula hispanica JCM 8911T.
33095133	9	37	theme	phosphate	857:865	arg1	ester					874:878	phosphatidylglycerol phosphate methyl ester	836:878	phosphatidylglycerol phosphate methyl ester	836:878	The polar lipids of strain MD130-1T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and triglycosyl diether-2.
33095133	10	38	theme	name	1044:1047	arg1	sp					1074:1075	the name Haloarcula mannanilytica sp	1040:1075	the name Haloarcula mannanilytica sp	1040:1075	Based on the results of phenotypic and phylogenetic analyses, the strain represents a new species of the genus Haloarcula, for which the name Haloarcula mannanilytica sp.
33095133	7	39	theme	JCM	582:584	arg1	7785T					586:590	Haloarcula japonica JCM 7785T	562:590	Haloarcula japonica JCM 7785T	562:590	The orthologous 16S rRNA gene sequence showed the highest similarity (99.4 %) to those of Haloarcula japonica JCM 7785T and Haloarcula hispanica JCM 8911T.
33095133	12	40	theme	=JCM	1125:1128	arg1	MD130-1T					1115:1122	MD130-1T	1115:1122	MD130-1T (=JCM 33835T=KCTC 4287T) isolated from commercial salt made in Ishikawa prefecture, Japan	1115:1212	The type strain is MD130-1T (=JCM 33835T=KCTC 4287T) isolated from commercial salt made in Ishikawa prefecture, Japan.
33095133	12	40	theme	=JCM	1125:1128	arg1	4287T					1142:1146	=JCM 33835T=KCTC 4287T	1125:1146	=JCM 33835T=KCTC 4287T	1125:1146	The type strain is MD130-1T (=JCM 33835T=KCTC 4287T) isolated from commercial salt made in Ishikawa prefecture, Japan.
33095133	10	41	theme	analyses	959:966	arg1	results					920:926	the results	916:926	the results of phenotypic and phylogenetic analyses	916:966	Based on the results of phenotypic and phylogenetic analyses, the strain represents a new species of the genus Haloarcula, for which the name Haloarcula mannanilytica sp.
33095133	2	42	attach	isolated	166:173	arg2	MD130-1T					152:159	MD130-1T	152:159	MD130-1T	152:159	A mannan-degrading halophilic archaeal strain, MD130-1T, was isolated from a commercial salt sample.
33095133	2	42	attach	isolated	166:173	arg1	sample					198:203	a commercial salt sample	180:203	a commercial salt sample	180:203	A mannan-degrading halophilic archaeal strain, MD130-1T, was isolated from a commercial salt sample.
33095133	2	42	attach	isolated	166:173	arg2	strain					144:149	A mannan-degrading halophilic archaeal strain	105:149	A mannan-degrading halophilic archaeal strain	105:149	A mannan-degrading halophilic archaeal strain, MD130-1T, was isolated from a commercial salt sample.
33095133	12	43	theme	type	1100:1103	arg1	MD130-1T					1115:1122	MD130-1T	1115:1122	MD130-1T (=JCM 33835T=KCTC 4287T) isolated from commercial salt made in Ishikawa prefecture, Japan	1115:1212	The type strain is MD130-1T (=JCM 33835T=KCTC 4287T) isolated from commercial salt made in Ishikawa prefecture, Japan.
33095133	12	43	theme	type	1100:1103	arg1	strain					1105:1110	The type strain	1096:1110	The type strain	1096:1110	The type strain is MD130-1T (=JCM 33835T=KCTC 4287T) isolated from commercial salt made in Ishikawa prefecture, Japan.
33095133	9	44	theme	polar	777:781	arg1	phosphatidylglycerol					814:833	phosphatidylglycerol	814:833	phosphatidylglycerol	814:833	The polar lipids of strain MD130-1T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and triglycosyl diether-2.
33095133	9	44	theme	polar	777:781	arg1	lipids					783:788	The polar lipids	773:788	The polar lipids of strain MD130-1T	773:807	The polar lipids of strain MD130-1T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and triglycosyl diether-2.
33095133	7	45	theme	Haloarcula	562:571	arg1	7785T					586:590	Haloarcula japonica JCM 7785T	562:590	Haloarcula japonica JCM 7785T	562:590	The orthologous 16S rRNA gene sequence showed the highest similarity (99.4 %) to those of Haloarcula japonica JCM 7785T and Haloarcula hispanica JCM 8911T.
33095133	8	46	theme	relatedness	649:659	arg1	%					725:725	84.33-85.96 %	713:725	84.33-85.96 % in ANIb	713:733	The values of genome relatedness between strain MD130-1T and Haloarcula species were 84.33-85.96 % in ANIb and 30.4-32.9 % using GGDC formula 2.
33095133	8	46	theme	relatedness	649:659	arg1	values					632:637	The values	628:637	The values of genome relatedness between strain MD130-1T and Haloarcula species	628:706	The values of genome relatedness between strain MD130-1T and Haloarcula species were 84.33-85.96 % in ANIb and 30.4-32.9 % using GGDC formula 2.
33095133	7	47	theme	rRNA	492:495	arg1	gene					497:500	The orthologous 16S rRNA gene	472:500	The orthologous 16S rRNA gene sequence	472:509	The orthologous 16S rRNA gene sequence showed the highest similarity (99.4 %) to those of Haloarcula japonica JCM 7785T and Haloarcula hispanica JCM 8911T.
33095133	5	48	dep	optimum	346:352	arg1	M					359:359	3.6 M	355:359	3.6 M	355:359	Strain MD130-1T was able to grow at 1.5-4.6 M NaCl (optimum, 3.6 M) at pH 6.0-8.0 (optimum, pH 7.0) and at 25-50 °C (optimum, 40 °C).
33095133	5	49	dep	optimum	411:417	arg1	40 °C					420:424	40 °C	420:424	40 °C	420:424	Strain MD130-1T was able to grow at 1.5-4.6 M NaCl (optimum, 3.6 M) at pH 6.0-8.0 (optimum, pH 7.0) and at 25-50 °C (optimum, 40 °C).
33095133	12	50	theme	33835T=KCTC	1130:1140	arg1	MD130-1T					1115:1122	MD130-1T	1115:1122	MD130-1T (=JCM 33835T=KCTC 4287T) isolated from commercial salt made in Ishikawa prefecture, Japan	1115:1212	The type strain is MD130-1T (=JCM 33835T=KCTC 4287T) isolated from commercial salt made in Ishikawa prefecture, Japan.
33095133	12	50	theme	33835T=KCTC	1130:1140	arg1	4287T					1142:1146	=JCM 33835T=KCTC 4287T	1125:1146	=JCM 33835T=KCTC 4287T	1125:1146	The type strain is MD130-1T (=JCM 33835T=KCTC 4287T) isolated from commercial salt made in Ishikawa prefecture, Japan.
33095133	8	51	theme	genome	642:647	arg1	relatedness					649:659	genome relatedness	642:659	genome relatedness	642:659	The values of genome relatedness between strain MD130-1T and Haloarcula species were 84.33-85.96 % in ANIb and 30.4-32.9 % using GGDC formula 2.
33095133	8	52	theme	strain	669:674	arg1	MD130-1T					676:683	strain MD130-1T	669:683	strain MD130-1T	669:683	The values of genome relatedness between strain MD130-1T and Haloarcula species were 84.33-85.96 % in ANIb and 30.4-32.9 % using GGDC formula 2.
33095133	9	53	theme	strain	793:798	arg1	MD130-1T					800:807	strain MD130-1T	793:807	strain MD130-1T	793:807	The polar lipids of strain MD130-1T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and triglycosyl diether-2.
33095133	1	54	theme	galactomannan-degrading	37:59	arg1	haloarchaeon					61:72	a galactomannan-degrading haloarchaeon	35:72	a galactomannan-degrading haloarchaeon	35:72	nov., a galactomannan-degrading haloarchaeon isolated from commercial salt.
33095133	1	54	theme	galactomannan-degrading	37:59	arg1	nov.					29:32	nov.	29:32	nov.	29:32	nov., a galactomannan-degrading haloarchaeon isolated from commercial salt.
33095133	8	55	theme	Haloarcula	689:698	arg1	species					700:706	Haloarcula species	689:706	Haloarcula species	689:706	The values of genome relatedness between strain MD130-1T and Haloarcula species were 84.33-85.96 % in ANIb and 30.4-32.9 % using GGDC formula 2.
33095133	9	56	theme	methyl	867:872	arg1	ester					874:878	phosphatidylglycerol phosphate methyl ester	836:878	phosphatidylglycerol phosphate methyl ester	836:878	The polar lipids of strain MD130-1T were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and triglycosyl diether-2.
33095133	10	57	theme	mannanilytica	1060:1072	arg1	sp					1074:1075	the name Haloarcula mannanilytica sp	1040:1075	the name Haloarcula mannanilytica sp	1040:1075	Based on the results of phenotypic and phylogenetic analyses, the strain represents a new species of the genus Haloarcula, for which the name Haloarcula mannanilytica sp.
32507162	0	0	theme	bone	94:97	arg1	regeneration					99:110	guided bone regeneration	87:110	guided bone regeneration	87:110	Glycerylphytate crosslinker as a potential osteoinductor of chitosan-based systems for guided bone regeneration.
32507162	4	1	theme	composition	560:570	arg1	terms					551:555	terms	551:555	terms of composition, morphology, and thermal behavior	551:604	Physico-chemical characterization in terms of composition, morphology, and thermal behavior was further analyzed.
32507162	7	2	theme	stem	1176:1179	arg1	cells					1181:1185	cultured stem cells	1167:1185	cultured stem cells	1167:1185	Stem cells cultured on the membranes increased their viability over time, and the incorporation of glycerylphytate improved osteogenic and osteoinductivity potential of chitosan by increasing calcium deposition and alkaline phosphatase (ALP) activity on cultured stem cells.
32507162	0	3	theme	guided	87:92	arg1	regeneration					99:110	guided bone regeneration	87:110	guided bone regeneration	87:110	Glycerylphytate crosslinker as a potential osteoinductor of chitosan-based systems for guided bone regeneration.
32507162	7	4	theme	calcium	1105:1111	arg1	deposition					1113:1122	calcium deposition	1105:1122	calcium deposition	1105:1122	Stem cells cultured on the membranes increased their viability over time, and the incorporation of glycerylphytate improved osteogenic and osteoinductivity potential of chitosan by increasing calcium deposition and alkaline phosphatase (ALP) activity on cultured stem cells.
32507162	4	5	theme	morphology	573:582	arg1	terms					551:555	terms	551:555	terms of composition, morphology, and thermal behavior	551:604	Physico-chemical characterization in terms of composition, morphology, and thermal behavior was further analyzed.
32507162	7	6	theme	alkaline	1128:1135	arg1	phosphatase					1137:1147	alkaline phosphatase	1128:1147	alkaline phosphatase (ALP) activity	1128:1162	Stem cells cultured on the membranes increased their viability over time, and the incorporation of glycerylphytate improved osteogenic and osteoinductivity potential of chitosan by increasing calcium deposition and alkaline phosphatase (ALP) activity on cultured stem cells.
32507162	7	6	theme	alkaline	1128:1135	arg1	ALP					1150:1152	ALP	1150:1152	ALP	1150:1152	Stem cells cultured on the membranes increased their viability over time, and the incorporation of glycerylphytate improved osteogenic and osteoinductivity potential of chitosan by increasing calcium deposition and alkaline phosphatase (ALP) activity on cultured stem cells.
32507162	7	7	theme	phosphatase	1137:1147	arg1	activity					1155:1162	alkaline phosphatase (ALP) activity	1128:1162	alkaline phosphatase (ALP) activity	1128:1162	Stem cells cultured on the membranes increased their viability over time, and the incorporation of glycerylphytate improved osteogenic and osteoinductivity potential of chitosan by increasing calcium deposition and alkaline phosphatase (ALP) activity on cultured stem cells.
32507162	3	8	link	glycerylphytate-crosslinked	379:405	arg1	membranes					407:415	Three different glycerylphytate-crosslinked membranes	363:415	Three different glycerylphytate-crosslinked membranes	363:415	Three different glycerylphytate-crosslinked membranes were developed by changing the crosslinker concentration, from 2.5-10 wt-%, respect to chitosan.
32507162	5	9	theme	crosslinker	671:681	arg1	release					683:689	crosslinker release	671:689	crosslinker release	671:689	Swelling degree, crosslinking density, and crosslinker release showed a glycerylphytate content-dependent behavior.
32507162	2	10	theme	membranes	307:315	arg1	fabrication					283:293	the fabrication	279:293	the fabrication of chitosan membranes	279:315	In this work, we used glycerylphytate as ionic crosslinker and osteinductor compound for the fabrication of chitosan membranes as supports for human mesenchymal stem cells.
32507162	8	11	link	glycerylphytate-crosslinked	1242:1268	arg1	systems					1279:1285	glycerylphytate-crosslinked chitosan systems	1242:1285	glycerylphytate-crosslinked chitosan systems	1242:1285	These results demonstrated a potential application of glycerylphytate-crosslinked chitosan systems for promising bone tissue regeneration.
32507162	6	12	from	incubation	877:886	arg1	fluid					906:910	body simulated fluid	891:910	body simulated fluid	891:910	Glycerylphytate suggested to improve osteointegration ability of chitosan surfaces by the formation of apatite-like aggregates after incubation in body simulated fluid.
32507162	3	13	theme	different	369:377	arg1	membranes					407:415	Three different glycerylphytate-crosslinked membranes	363:415	Three different glycerylphytate-crosslinked membranes	363:415	Three different glycerylphytate-crosslinked membranes were developed by changing the crosslinker concentration, from 2.5-10 wt-%, respect to chitosan.
32507162	8	14	theme	bone	1301:1304	arg1	regeneration					1313:1324	promising bone tissue regeneration	1291:1324	promising bone tissue regeneration	1291:1324	These results demonstrated a potential application of glycerylphytate-crosslinked chitosan systems for promising bone tissue regeneration.
32507162	2	15	theme	chitosan	298:305	arg1	membranes					307:315	chitosan membranes	298:315	chitosan membranes	298:315	In this work, we used glycerylphytate as ionic crosslinker and osteinductor compound for the fabrication of chitosan membranes as supports for human mesenchymal stem cells.
32507162	6	16	theme	surfaces	818:825	arg1	ability					798:804	osteointegration ability	781:804	osteointegration ability of chitosan surfaces	781:825	Glycerylphytate suggested to improve osteointegration ability of chitosan surfaces by the formation of apatite-like aggregates after incubation in body simulated fluid.
32507162	3	17	theme	glycerylphytate-crosslinked	379:405	arg1	membranes					407:415	Three different glycerylphytate-crosslinked membranes	363:415	Three different glycerylphytate-crosslinked membranes	363:415	Three different glycerylphytate-crosslinked membranes were developed by changing the crosslinker concentration, from 2.5-10 wt-%, respect to chitosan.
32507162	6	18	theme	chitosan	809:816	arg1	surfaces					818:825	chitosan surfaces	809:825	chitosan surfaces	809:825	Glycerylphytate suggested to improve osteointegration ability of chitosan surfaces by the formation of apatite-like aggregates after incubation in body simulated fluid.
32507162	5	19	theme	glycerylphytate	700:714	arg1	behavior					734:741	a glycerylphytate content-dependent behavior	698:741	a glycerylphytate content-dependent behavior	698:741	Swelling degree, crosslinking density, and crosslinker release showed a glycerylphytate content-dependent behavior.
32507162	7	20	theme	chitosan	1082:1089	arg1	potential					1069:1077	osteogenic and osteoinductivity potential	1037:1077	potential	1069:1077	Stem cells cultured on the membranes increased their viability over time, and the incorporation of glycerylphytate improved osteogenic and osteoinductivity potential of chitosan by increasing calcium deposition and alkaline phosphatase (ALP) activity on cultured stem cells.
32507162	2	21	theme	mesenchymal	339:349	arg1	cells					356:360	human mesenchymal stem cells	333:360	human mesenchymal stem cells	333:360	In this work, we used glycerylphytate as ionic crosslinker and osteinductor compound for the fabrication of chitosan membranes as supports for human mesenchymal stem cells.
32507162	4	22	from	characterization	531:546	arg1	terms					551:555	terms	551:555	terms of composition, morphology, and thermal behavior	551:604	Physico-chemical characterization in terms of composition, morphology, and thermal behavior was further analyzed.
32507162	5	23	theme	crosslinking	645:656	arg1	density					658:664	crosslinking density	645:664	crosslinking density	645:664	Swelling degree, crosslinking density, and crosslinker release showed a glycerylphytate content-dependent behavior.
32507162	2	24	theme	human	333:337	arg1	cells					356:360	human mesenchymal stem cells	333:360	human mesenchymal stem cells	333:360	In this work, we used glycerylphytate as ionic crosslinker and osteinductor compound for the fabrication of chitosan membranes as supports for human mesenchymal stem cells.
32507162	6	25	theme	simulated	896:904	arg1	fluid					906:910	body simulated fluid	891:910	body simulated fluid	891:910	Glycerylphytate suggested to improve osteointegration ability of chitosan surfaces by the formation of apatite-like aggregates after incubation in body simulated fluid.
32507162	6	26	theme	body	891:894	arg1	fluid					906:910	body simulated fluid	891:910	body simulated fluid	891:910	Glycerylphytate suggested to improve osteointegration ability of chitosan surfaces by the formation of apatite-like aggregates after incubation in body simulated fluid.
32507162	1	27	theme	Chitosan-based	113:126	arg1	systems					152:158	promising systems	142:158	promising systems for guided bone regeneration	142:187	Chitosan-based membranes are promising systems for guided bone regeneration.
32507162	1	27	theme	Chitosan-based	113:126	arg1	membranes					128:136	Chitosan-based membranes	113:136	Chitosan-based membranes	113:136	Chitosan-based membranes are promising systems for guided bone regeneration.
32507162	6	28	theme	osteointegration	781:796	arg1	ability					798:804	osteointegration ability	781:804	osteointegration ability of chitosan surfaces	781:825	Glycerylphytate suggested to improve osteointegration ability of chitosan surfaces by the formation of apatite-like aggregates after incubation in body simulated fluid.
32507162	7	29	theme	Stem	913:916	arg1	cells					918:922	Stem cells	913:922	Stem cells cultured on the membranes	913:948	Stem cells cultured on the membranes increased their viability over time, and the incorporation of glycerylphytate improved osteogenic and osteoinductivity potential of chitosan by increasing calcium deposition and alkaline phosphatase (ALP) activity on cultured stem cells.
32507162	8	30	theme	potential	1217:1225	arg1	application					1227:1237	a potential application	1215:1237	a potential application of glycerylphytate-crosslinked chitosan systems for promising bone tissue regeneration	1215:1324	These results demonstrated a potential application of glycerylphytate-crosslinked chitosan systems for promising bone tissue regeneration.
32507162	0	31	theme	systems	75:81	arg1	osteoinductor					43:55	a potential osteoinductor	31:55	a potential osteoinductor of chitosan-based systems for guided bone regeneration	31:110	Glycerylphytate crosslinker as a potential osteoinductor of chitosan-based systems for guided bone regeneration.
32507162	0	31	theme	systems	75:81	arg1	crosslinker					16:26	crosslinker	16:26	crosslinker	16:26	Glycerylphytate crosslinker as a potential osteoinductor of chitosan-based systems for guided bone regeneration.
32507162	8	32	theme	glycerylphytate-crosslinked	1242:1268	arg1	systems					1279:1285	glycerylphytate-crosslinked chitosan systems	1242:1285	glycerylphytate-crosslinked chitosan systems	1242:1285	These results demonstrated a potential application of glycerylphytate-crosslinked chitosan systems for promising bone tissue regeneration.
32507162	2	33	theme	osteinductor	253:264	arg1	compound					266:273	osteinductor compound	253:273	osteinductor compound	253:273	In this work, we used glycerylphytate as ionic crosslinker and osteinductor compound for the fabrication of chitosan membranes as supports for human mesenchymal stem cells.
32507162	0	34	theme	potential	33:41	arg1	osteoinductor					43:55	a potential osteoinductor	31:55	a potential osteoinductor of chitosan-based systems for guided bone regeneration	31:110	Glycerylphytate crosslinker as a potential osteoinductor of chitosan-based systems for guided bone regeneration.
32507162	0	34	theme	potential	33:41	arg1	crosslinker					16:26	crosslinker	16:26	crosslinker	16:26	Glycerylphytate crosslinker as a potential osteoinductor of chitosan-based systems for guided bone regeneration.
32507162	4	35	theme	thermal	589:595	arg1	behavior					597:604	thermal behavior	589:604	thermal behavior	589:604	Physico-chemical characterization in terms of composition, morphology, and thermal behavior was further analyzed.
32507162	4	36	theme	Physico-chemical	514:529	arg1	characterization					531:546	Physico-chemical characterization	514:546	Physico-chemical characterization in terms of composition, morphology, and thermal behavior	514:604	Physico-chemical characterization in terms of composition, morphology, and thermal behavior was further analyzed.
32507162	3	37	theme	crosslinker	448:458	arg1	concentration					460:472	the crosslinker concentration	444:472	the crosslinker concentration	444:472	Three different glycerylphytate-crosslinked membranes were developed by changing the crosslinker concentration, from 2.5-10 wt-%, respect to chitosan.
32507162	8	38	theme	systems	1279:1285	arg1	application					1227:1237	a potential application	1215:1237	a potential application of glycerylphytate-crosslinked chitosan systems for promising bone tissue regeneration	1215:1324	These results demonstrated a potential application of glycerylphytate-crosslinked chitosan systems for promising bone tissue regeneration.
32507162	2	39	used	used	207:210	arg2	we					204:205	we	204:205	we	204:205	In this work, we used glycerylphytate as ionic crosslinker and osteinductor compound for the fabrication of chitosan membranes as supports for human mesenchymal stem cells.
32507162	8	40	theme	promising	1291:1299	arg1	regeneration					1313:1324	promising bone tissue regeneration	1291:1324	promising bone tissue regeneration	1291:1324	These results demonstrated a potential application of glycerylphytate-crosslinked chitosan systems for promising bone tissue regeneration.
32507162	8	41	theme	chitosan	1270:1277	arg1	systems					1279:1285	glycerylphytate-crosslinked chitosan systems	1242:1285	glycerylphytate-crosslinked chitosan systems	1242:1285	These results demonstrated a potential application of glycerylphytate-crosslinked chitosan systems for promising bone tissue regeneration.
32507162	2	42	theme	ionic	231:235	arg1	crosslinker					237:247	ionic crosslinker	231:247	ionic crosslinker	231:247	In this work, we used glycerylphytate as ionic crosslinker and osteinductor compound for the fabrication of chitosan membranes as supports for human mesenchymal stem cells.
32507162	1	43	theme	promising	142:150	arg1	systems					152:158	promising systems	142:158	promising systems for guided bone regeneration	142:187	Chitosan-based membranes are promising systems for guided bone regeneration.
32507162	1	43	theme	promising	142:150	arg1	membranes					128:136	Chitosan-based membranes	113:136	Chitosan-based membranes	113:136	Chitosan-based membranes are promising systems for guided bone regeneration.
32507162	7	44	theme	osteogenic	1037:1046	arg1	potential					1069:1077	osteogenic and osteoinductivity potential	1037:1077	potential	1069:1077	Stem cells cultured on the membranes increased their viability over time, and the incorporation of glycerylphytate improved osteogenic and osteoinductivity potential of chitosan by increasing calcium deposition and alkaline phosphatase (ALP) activity on cultured stem cells.
32507162	4	45	theme	behavior	597:604	arg1	terms					551:555	terms	551:555	terms of composition, morphology, and thermal behavior	551:604	Physico-chemical characterization in terms of composition, morphology, and thermal behavior was further analyzed.
32507162	7	46	theme	cultured	1167:1174	arg1	cells					1181:1185	cultured stem cells	1167:1185	cultured stem cells	1167:1185	Stem cells cultured on the membranes increased their viability over time, and the incorporation of glycerylphytate improved osteogenic and osteoinductivity potential of chitosan by increasing calcium deposition and alkaline phosphatase (ALP) activity on cultured stem cells.
32507162	5	47	theme	Swelling	628:635	arg1	degree					637:642	Swelling degree	628:642	Swelling degree	628:642	Swelling degree, crosslinking density, and crosslinker release showed a glycerylphytate content-dependent behavior.
32507162	6	48	theme	aggregates	860:869	arg1	formation					834:842	the formation	830:842	the formation of apatite-like aggregates	830:869	Glycerylphytate suggested to improve osteointegration ability of chitosan surfaces by the formation of apatite-like aggregates after incubation in body simulated fluid.
32507162	6	49	theme	apatite-like	847:858	arg1	aggregates					860:869	apatite-like aggregates	847:869	apatite-like aggregates	847:869	Glycerylphytate suggested to improve osteointegration ability of chitosan surfaces by the formation of apatite-like aggregates after incubation in body simulated fluid.
32507162	2	50	theme	stem	351:354	arg1	cells					356:360	human mesenchymal stem cells	333:360	human mesenchymal stem cells	333:360	In this work, we used glycerylphytate as ionic crosslinker and osteinductor compound for the fabrication of chitosan membranes as supports for human mesenchymal stem cells.
32507162	7	51	theme	glycerylphytate	1012:1026	arg1	incorporation					995:1007	the incorporation	991:1007	the incorporation of glycerylphytate	991:1026	Stem cells cultured on the membranes increased their viability over time, and the incorporation of glycerylphytate improved osteogenic and osteoinductivity potential of chitosan by increasing calcium deposition and alkaline phosphatase (ALP) activity on cultured stem cells.
32507162	8	52	theme	tissue	1306:1311	arg1	regeneration					1313:1324	promising bone tissue regeneration	1291:1324	promising bone tissue regeneration	1291:1324	These results demonstrated a potential application of glycerylphytate-crosslinked chitosan systems for promising bone tissue regeneration.
32507162	1	53	theme	guided	164:169	arg1	regeneration					176:187	guided bone regeneration	164:187	guided bone regeneration	164:187	Chitosan-based membranes are promising systems for guided bone regeneration.
32507162	0	54	theme	chitosan-based	60:73	arg1	systems					75:81	chitosan-based systems	60:81	chitosan-based systems	60:81	Glycerylphytate crosslinker as a potential osteoinductor of chitosan-based systems for guided bone regeneration.
32507162	1	55	theme	bone	171:174	arg1	regeneration					176:187	guided bone regeneration	164:187	guided bone regeneration	164:187	Chitosan-based membranes are promising systems for guided bone regeneration.
32507162	5	56	theme	content-dependent	716:732	arg1	behavior					734:741	a glycerylphytate content-dependent behavior	698:741	a glycerylphytate content-dependent behavior	698:741	Swelling degree, crosslinking density, and crosslinker release showed a glycerylphytate content-dependent behavior.
32507162	7	57	theme	osteoinductivity	1052:1067	arg1	potential					1069:1077	osteogenic and osteoinductivity potential	1037:1077	potential	1069:1077	Stem cells cultured on the membranes increased their viability over time, and the incorporation of glycerylphytate improved osteogenic and osteoinductivity potential of chitosan by increasing calcium deposition and alkaline phosphatase (ALP) activity on cultured stem cells.
32617606	3	0	theme	strain	292:297	arg1	H1T					299:301	strain H1T	292:301	strain H1T	292:301	The 16S rRNA gene sequence result indicated that strain H1T shared the highest 16S rRNA gene sequence identity with the type strain of Deinococcus alpinitundrae (96.2%).
32617606	2	1	theme	coccus	113:118	arg1	bacterium					140:148	A Gram-staining-positive, non-motile, coccus or short-rod-shaped bacterium	75:148	A Gram-staining-positive, non-motile, coccus or short-rod-shaped bacterium	75:148	A Gram-staining-positive, non-motile, coccus or short-rod-shaped bacterium, designated H1T, was isolated from a humus soil sample in the Detaille Island of Antarctica.
32617606	10	2	theme	average	822:828	arg1	identity					841:848	The average nucleotide identity	818:848	The average nucleotide identity (ANI) between strain H1T and the closely related Deinococcus members	818:917	The average nucleotide identity (ANI) between strain H1T and the closely related Deinococcus members was below the cut-off level (95-96%) for species identification.
32617606	10	2	theme	average	822:828	arg1	ANI					851:853	ANI	851:853	ANI	851:853	The average nucleotide identity (ANI) between strain H1T and the closely related Deinococcus members was below the cut-off level (95-96%) for species identification.
32617606	10	3	theme	species	960:966	arg1	identification					968:981	species identification	960:981	species identification	960:981	The average nucleotide identity (ANI) between strain H1T and the closely related Deinococcus members was below the cut-off level (95-96%) for species identification.
32617606	8	4	theme	peptidoglycan	745:757	arg1	type					759:762	The cell wall peptidoglycan type	731:762	The cell wall peptidoglycan type	731:762	The cell wall peptidoglycan type was A3β.
32617606	8	4	theme	peptidoglycan	745:757	arg1	A3β					768:770	A3β	768:770	A3β	768:770	The cell wall peptidoglycan type was A3β.
32617606	11	5	theme	name	1090:1093	arg1	sp					1120:1121	the name Deinococcus detaillensis sp	1086:1121	the name Deinococcus detaillensis sp	1086:1121	Based on the above results, strain H1T represents a novel species of the genus Deinococcus, for which the name Deinococcus detaillensis sp.
32617606	13	6	theme	= CGMCC	1162:1168	arg1	33291T					1185:1190	= CGMCC 1.13938T = JCM 33291T	1162:1190	= CGMCC 1.13938T = JCM 33291T	1162:1190	Type strain is H1T (= CGMCC 1.13938T = JCM 33291T).
32617606	13	6	theme	= CGMCC	1162:1168	arg1	H1T					1157:1159	H1T	1157:1159	H1T (= CGMCC 1.13938T = JCM 33291T)	1157:1191	Type strain is H1T (= CGMCC 1.13938T = JCM 33291T).
32617606	3	7	theme	rRNA	326:329	arg1	identity					345:352	the highest 16S rRNA gene sequence identity	310:352	the highest 16S rRNA gene sequence identity	310:352	The 16S rRNA gene sequence result indicated that strain H1T shared the highest 16S rRNA gene sequence identity with the type strain of Deinococcus alpinitundrae (96.2%).
32617606	2	8	theme	non-motile	101:110	arg1	bacterium					140:148	A Gram-staining-positive, non-motile, coccus or short-rod-shaped bacterium	75:148	A Gram-staining-positive, non-motile, coccus or short-rod-shaped bacterium	75:148	A Gram-staining-positive, non-motile, coccus or short-rod-shaped bacterium, designated H1T, was isolated from a humus soil sample in the Detaille Island of Antarctica.
32617606	5	9	theme	respiratory	512:522	arg1	MK-8					536:539	MK-8	536:539	MK-8	536:539	The respiratory quinone was MK-8.
32617606	5	9	theme	respiratory	512:522	arg1	quinone					524:530	The respiratory quinone	508:530	The respiratory quinone	508:530	The respiratory quinone was MK-8.
32617606	1	10	theme	humus	49:53	arg1	soil					55:58	humus soil	49:58	humus soil in Antarctica	49:72	nov., isolated from humus soil in Antarctica.
32617606	1	11	from	soil	55:58	arg1	Antarctica					63:72	Antarctica	63:72	Antarctica	63:72	nov., isolated from humus soil in Antarctica.
32617606	13	12	theme	1.13938T = JCM	1170:1183	arg1	33291T					1185:1190	= CGMCC 1.13938T = JCM 33291T	1162:1190	= CGMCC 1.13938T = JCM 33291T	1162:1190	Type strain is H1T (= CGMCC 1.13938T = JCM 33291T).
32617606	13	12	theme	1.13938T = JCM	1170:1183	arg1	H1T					1157:1159	H1T	1157:1159	H1T (= CGMCC 1.13938T = JCM 33291T)	1157:1191	Type strain is H1T (= CGMCC 1.13938T = JCM 33291T).
32617606	3	13	theme	gene	331:334	arg1	identity					345:352	the highest 16S rRNA gene sequence identity	310:352	the highest 16S rRNA gene sequence identity	310:352	The 16S rRNA gene sequence result indicated that strain H1T shared the highest 16S rRNA gene sequence identity with the type strain of Deinococcus alpinitundrae (96.2%).
32617606	4	14	theme	H1T	430:432	arg1	Growth					413:418	Growth	413:418	Growth of strain H1T	413:432	Growth of strain H1T occurred at 4-25 °C, pH 6.0-8.0 and in the presence of 0-1.0% NaCl (w/v).
32617606	3	15	theme	highest	314:320	arg1	rRNA					326:329	the highest 16S rRNA	310:329	the highest 16S rRNA gene sequence identity	310:352	The 16S rRNA gene sequence result indicated that strain H1T shared the highest 16S rRNA gene sequence identity with the type strain of Deinococcus alpinitundrae (96.2%).
32617606	4	16	theme	strain	423:428	arg1	H1T					430:432	strain H1T	423:432	strain H1T	423:432	Growth of strain H1T occurred at 4-25 °C, pH 6.0-8.0 and in the presence of 0-1.0% NaCl (w/v).
32617606	11	17	dep	Deinococcus	1095:1105	arg1	detaillensis					1107:1118	detaillensis	1107:1118	detaillensis	1107:1118	Based on the above results, strain H1T represents a novel species of the genus Deinococcus, for which the name Deinococcus detaillensis sp.
32617606	2	18	from	sample	198:203	arg1	Island					221:226	the Detaille Island	208:226	the Detaille Island of Antarctica	208:240	A Gram-staining-positive, non-motile, coccus or short-rod-shaped bacterium, designated H1T, was isolated from a humus soil sample in the Detaille Island of Antarctica.
32617606	3	19	theme	16S	322:324	arg1	rRNA					326:329	the highest 16S rRNA	310:329	the highest 16S rRNA gene sequence identity	310:352	The 16S rRNA gene sequence result indicated that strain H1T shared the highest 16S rRNA gene sequence identity with the type strain of Deinococcus alpinitundrae (96.2%).
32617606	11	20	theme	genus	1057:1061	arg1	Deinococcus					1063:1073	the genus Deinococcus	1053:1073	the genus Deinococcus	1053:1073	Based on the above results, strain H1T represents a novel species of the genus Deinococcus, for which the name Deinococcus detaillensis sp.
32617606	11	21	theme	novel	1036:1040	arg1	species					1042:1048	a novel species	1034:1048	a novel species	1034:1048	Based on the above results, strain H1T represents a novel species of the genus Deinococcus, for which the name Deinococcus detaillensis sp.
32617606	0	22	theme	detaillensis	12:23	arg1	sp					25:26	Deinococcus detaillensis sp	0:26	Deinococcus detaillensis sp.	0:27	Deinococcus detaillensis sp.
32617606	2	23	theme	short-rod-shaped	123:138	arg1	bacterium					140:148	A Gram-staining-positive, non-motile, coccus or short-rod-shaped bacterium	75:148	A Gram-staining-positive, non-motile, coccus or short-rod-shaped bacterium	75:148	A Gram-staining-positive, non-motile, coccus or short-rod-shaped bacterium, designated H1T, was isolated from a humus soil sample in the Detaille Island of Antarctica.
32617606	11	24	theme	Deinococcus	1063:1073	arg1	species					1042:1048	a novel species	1034:1048	a novel species	1034:1048	Based on the above results, strain H1T represents a novel species of the genus Deinococcus, for which the name Deinococcus detaillensis sp.
32617606	6	25	theme	summed	592:597	arg1	feature					599:605	summed feature 3	592:607	summed feature 3	592:607	The major fatty acids were C16:0, C17:0 cyclo and summed feature 3 (C16:1 ω7c/C16:1 ω6c).
32617606	6	25	theme	summed	592:597	arg1	C16:0					569:573	C16:0	569:573	C16:0	569:573	The major fatty acids were C16:0, C17:0 cyclo and summed feature 3 (C16:1 ω7c/C16:1 ω6c).
32617606	3	26	theme	alpinitundrae	390:402	arg1	strain					368:373	the type strain	359:373	the type strain of Deinococcus alpinitundrae (96.2%)	359:410	The 16S rRNA gene sequence result indicated that strain H1T shared the highest 16S rRNA gene sequence identity with the type strain of Deinococcus alpinitundrae (96.2%).
32617606	0	27	theme	Deinococcus	0:10	arg1	sp					25:26	Deinococcus detaillensis sp	0:26	Deinococcus detaillensis sp.	0:27	Deinococcus detaillensis sp.
32617606	4	28	theme	0-1.0	489:493	arg1	%					494:494	%	494:494	%	494:494	Growth of strain H1T occurred at 4-25 °C, pH 6.0-8.0 and in the presence of 0-1.0% NaCl (w/v).
32617606	3	29	theme	sequence	336:343	arg1	identity					345:352	the highest 16S rRNA gene sequence identity	310:352	the highest 16S rRNA gene sequence identity	310:352	The 16S rRNA gene sequence result indicated that strain H1T shared the highest 16S rRNA gene sequence identity with the type strain of Deinococcus alpinitundrae (96.2%).
32617606	4	30	dep	occurred	434:441	arg1	w/v					502:504	w/v	502:504	w/v	502:504	Growth of strain H1T occurred at 4-25 °C, pH 6.0-8.0 and in the presence of 0-1.0% NaCl (w/v).
32617606	3	31	theme	rRNA	251:254	arg1	result					270:275	The 16S rRNA gene sequence result	243:275	The 16S rRNA gene sequence result	243:275	The 16S rRNA gene sequence result indicated that strain H1T shared the highest 16S rRNA gene sequence identity with the type strain of Deinococcus alpinitundrae (96.2%).
32617606	11	32	theme	above	997:1001	arg1	results					1003:1009	the above results	993:1009	the above results	993:1009	Based on the above results, strain H1T represents a novel species of the genus Deinococcus, for which the name Deinococcus detaillensis sp.
32617606	4	33	theme	NaCl	496:499	arg1	presence					477:484	the presence	473:484	the presence of 0-1.0% NaCl	473:499	Growth of strain H1T occurred at 4-25 °C, pH 6.0-8.0 and in the presence of 0-1.0% NaCl (w/v).
32617606	3	34	theme	gene	256:259	arg1	result					270:275	The 16S rRNA gene sequence result	243:275	The 16S rRNA gene sequence result	243:275	The 16S rRNA gene sequence result indicated that strain H1T shared the highest 16S rRNA gene sequence identity with the type strain of Deinococcus alpinitundrae (96.2%).
32617606	13	35	theme	Type	1142:1145	arg1	strain					1147:1152	Type strain	1142:1152	Type strain	1142:1152	Type strain is H1T (= CGMCC 1.13938T = JCM 33291T).
32617606	10	36	theme	nucleotide	830:839	arg1	identity					841:848	The average nucleotide identity	818:848	The average nucleotide identity (ANI) between strain H1T and the closely related Deinococcus members	818:917	The average nucleotide identity (ANI) between strain H1T and the closely related Deinococcus members was below the cut-off level (95-96%) for species identification.
32617606	10	36	theme	nucleotide	830:839	arg1	ANI					851:853	ANI	851:853	ANI	851:853	The average nucleotide identity (ANI) between strain H1T and the closely related Deinococcus members was below the cut-off level (95-96%) for species identification.
32617606	6	37	theme	C17:0	576:580	arg1	cyclo					582:586	C17:0 cyclo	576:586	C17:0 cyclo	576:586	The major fatty acids were C16:0, C17:0 cyclo and summed feature 3 (C16:1 ω7c/C16:1 ω6c).
32617606	6	37	theme	C17:0	576:580	arg1	ω6c					626:628	ω6c	626:628	ω6c	626:628	The major fatty acids were C16:0, C17:0 cyclo and summed feature 3 (C16:1 ω7c/C16:1 ω6c).
32617606	6	37	theme	C17:0	576:580	arg1	C16:0					569:573	C16:0	569:573	C16:0	569:573	The major fatty acids were C16:0, C17:0 cyclo and summed feature 3 (C16:1 ω7c/C16:1 ω6c).
32617606	2	38	attach	isolated	171:178	arg2	bacterium					140:148	A Gram-staining-positive, non-motile, coccus or short-rod-shaped bacterium	75:148	A Gram-staining-positive, non-motile, coccus or short-rod-shaped bacterium	75:148	A Gram-staining-positive, non-motile, coccus or short-rod-shaped bacterium, designated H1T, was isolated from a humus soil sample in the Detaille Island of Antarctica.
32617606	2	38	attach	isolated	171:178	arg1	sample					198:203	a humus soil sample	185:203	a humus soil sample in the Detaille Island of Antarctica	185:240	A Gram-staining-positive, non-motile, coccus or short-rod-shaped bacterium, designated H1T, was isolated from a humus soil sample in the Detaille Island of Antarctica.
32617606	4	39	theme	%	494:494	arg1	NaCl					496:499	0-1.0% NaCl	489:499	0-1.0% NaCl	489:499	Growth of strain H1T occurred at 4-25 °C, pH 6.0-8.0 and in the presence of 0-1.0% NaCl (w/v).
32617606	9	40	theme	genomic	777:783	arg1	content					795:801	The genomic DNA G + C content	773:801	The genomic DNA G + C content	773:801	The genomic DNA G + C content was 61.3 mol%.
32617606	9	40	theme	genomic	777:783	arg1	%					815:815	61.3 mol%	807:815	61.3 mol%	807:815	The genomic DNA G + C content was 61.3 mol%.
32617606	2	41	theme	Detaille	212:219	arg1	Island					221:226	the Detaille Island	208:226	the Detaille Island of Antarctica	208:240	A Gram-staining-positive, non-motile, coccus or short-rod-shaped bacterium, designated H1T, was isolated from a humus soil sample in the Detaille Island of Antarctica.
32617606	8	42	theme	cell	735:738	arg1	type					759:762	The cell wall peptidoglycan type	731:762	The cell wall peptidoglycan type	731:762	The cell wall peptidoglycan type was A3β.
32617606	8	42	theme	cell	735:738	arg1	A3β					768:770	A3β	768:770	A3β	768:770	The cell wall peptidoglycan type was A3β.
32617606	3	43	theme	16S	247:249	arg1	rRNA					251:254	The 16S rRNA	243:254	The 16S rRNA gene sequence result	243:275	The 16S rRNA gene sequence result indicated that strain H1T shared the highest 16S rRNA gene sequence identity with the type strain of Deinococcus alpinitundrae (96.2%).
32617606	10	44	theme	strain	864:869	arg1	H1T					871:873	strain H1T	864:873	strain H1T	864:873	The average nucleotide identity (ANI) between strain H1T and the closely related Deinococcus members was below the cut-off level (95-96%) for species identification.
32617606	3	45	theme	type	363:366	arg1	strain					368:373	the type strain	359:373	the type strain of Deinococcus alpinitundrae (96.2%)	359:410	The 16S rRNA gene sequence result indicated that strain H1T shared the highest 16S rRNA gene sequence identity with the type strain of Deinococcus alpinitundrae (96.2%).
32617606	2	46	theme	Gram-staining-positive	77:98	arg1	bacterium					140:148	A Gram-staining-positive, non-motile, coccus or short-rod-shaped bacterium	75:148	A Gram-staining-positive, non-motile, coccus or short-rod-shaped bacterium	75:148	A Gram-staining-positive, non-motile, coccus or short-rod-shaped bacterium, designated H1T, was isolated from a humus soil sample in the Detaille Island of Antarctica.
32617606	9	47	theme	G + C	789:793	arg1	content					795:801	The genomic DNA G + C content	773:801	The genomic DNA G + C content	773:801	The genomic DNA G + C content was 61.3 mol%.
32617606	9	47	theme	G + C	789:793	arg1	%					815:815	61.3 mol%	807:815	61.3 mol%	807:815	The genomic DNA G + C content was 61.3 mol%.
32617606	10	48	theme	Deinococcus	899:909	arg1	members					911:917	the closely related Deinococcus members	879:917	the closely related Deinococcus members	879:917	The average nucleotide identity (ANI) between strain H1T and the closely related Deinococcus members was below the cut-off level (95-96%) for species identification.
32617606	11	49	theme	Deinococcus	1095:1105	arg1	sp					1120:1121	the name Deinococcus detaillensis sp	1086:1121	the name Deinococcus detaillensis sp	1086:1121	Based on the above results, strain H1T represents a novel species of the genus Deinococcus, for which the name Deinococcus detaillensis sp.
32617606	9	50	theme	DNA	785:787	arg1	content					795:801	The genomic DNA G + C content	773:801	The genomic DNA G + C content	773:801	The genomic DNA G + C content was 61.3 mol%.
32617606	9	50	theme	DNA	785:787	arg1	%					815:815	61.3 mol%	807:815	61.3 mol%	807:815	The genomic DNA G + C content was 61.3 mol%.
32617606	7	51	dep	aminoglycophospholipid	654:675	arg1	aminophospholipid					678:694	aminophospholipid	678:694	aminophospholipid	678:694	The polar lipids were aminoglycophospholipid, aminophospholipid, glycolipid and glycophospholipid.
32617606	2	52	theme	soil	193:196	arg1	sample					198:203	a humus soil sample	185:203	a humus soil sample in the Detaille Island of Antarctica	185:240	A Gram-staining-positive, non-motile, coccus or short-rod-shaped bacterium, designated H1T, was isolated from a humus soil sample in the Detaille Island of Antarctica.
32617606	7	53	theme	aminoglycophospholipid	654:675	arg1	glycolipid					697:706	glycolipid	697:706	glycolipid	697:706	The polar lipids were aminoglycophospholipid, aminophospholipid, glycolipid and glycophospholipid.
32617606	7	53	theme	aminoglycophospholipid	654:675	arg1	lipids					642:647	The polar lipids	632:647	The polar lipids	632:647	The polar lipids were aminoglycophospholipid, aminophospholipid, glycolipid and glycophospholipid.
32617606	3	54	theme	sequence	261:268	arg1	result					270:275	The 16S rRNA gene sequence result	243:275	The 16S rRNA gene sequence result	243:275	The 16S rRNA gene sequence result indicated that strain H1T shared the highest 16S rRNA gene sequence identity with the type strain of Deinococcus alpinitundrae (96.2%).
32617606	2	55	theme	humus	187:191	arg1	sample					198:203	a humus soil sample	185:203	a humus soil sample in the Detaille Island of Antarctica	185:240	A Gram-staining-positive, non-motile, coccus or short-rod-shaped bacterium, designated H1T, was isolated from a humus soil sample in the Detaille Island of Antarctica.
32617606	10	56	theme	related	891:897	arg1	members					911:917	the closely related Deinococcus members	879:917	the closely related Deinococcus members	879:917	The average nucleotide identity (ANI) between strain H1T and the closely related Deinococcus members was below the cut-off level (95-96%) for species identification.
32617606	7	57	theme	polar	636:640	arg1	glycolipid					697:706	glycolipid	697:706	glycolipid	697:706	The polar lipids were aminoglycophospholipid, aminophospholipid, glycolipid and glycophospholipid.
32617606	7	57	theme	polar	636:640	arg1	lipids					642:647	The polar lipids	632:647	The polar lipids	632:647	The polar lipids were aminoglycophospholipid, aminophospholipid, glycolipid and glycophospholipid.
32617606	9	58	theme	61.3 mol	807:814	arg1	content					795:801	The genomic DNA G + C content	773:801	The genomic DNA G + C content	773:801	The genomic DNA G + C content was 61.3 mol%.
32617606	9	58	theme	61.3 mol	807:814	arg1	%					815:815	61.3 mol%	807:815	61.3 mol%	807:815	The genomic DNA G + C content was 61.3 mol%.
32617606	10	59	theme	cut-off	933:939	arg1	level					941:945	the cut-off level	929:945	the cut-off level	929:945	The average nucleotide identity (ANI) between strain H1T and the closely related Deinococcus members was below the cut-off level (95-96%) for species identification.
32617606	2	60	theme	Antarctica	231:240	arg1	Island					221:226	the Detaille Island	208:226	the Detaille Island of Antarctica	208:240	A Gram-staining-positive, non-motile, coccus or short-rod-shaped bacterium, designated H1T, was isolated from a humus soil sample in the Detaille Island of Antarctica.
32617606	8	61	theme	wall	740:743	arg1	type					759:762	The cell wall peptidoglycan type	731:762	The cell wall peptidoglycan type	731:762	The cell wall peptidoglycan type was A3β.
32617606	8	61	theme	wall	740:743	arg1	A3β					768:770	A3β	768:770	A3β	768:770	The cell wall peptidoglycan type was A3β.
32617606	6	62	theme	fatty	552:556	arg1	acids					558:562	The major fatty acids	542:562	The major fatty acids	542:562	The major fatty acids were C16:0, C17:0 cyclo and summed feature 3 (C16:1 ω7c/C16:1 ω6c).
32617606	6	62	theme	fatty	552:556	arg1	C16:0					569:573	C16:0	569:573	C16:0	569:573	The major fatty acids were C16:0, C17:0 cyclo and summed feature 3 (C16:1 ω7c/C16:1 ω6c).
32617606	11	63	theme	strain	1012:1017	arg1	H1T					1019:1021	strain H1T	1012:1021	strain H1T	1012:1021	Based on the above results, strain H1T represents a novel species of the genus Deinococcus, for which the name Deinococcus detaillensis sp.
32617606	6	64	theme	major	546:550	arg1	acids					558:562	The major fatty acids	542:562	The major fatty acids	542:562	The major fatty acids were C16:0, C17:0 cyclo and summed feature 3 (C16:1 ω7c/C16:1 ω6c).
32617606	6	64	theme	major	546:550	arg1	C16:0					569:573	C16:0	569:573	C16:0	569:573	The major fatty acids were C16:0, C17:0 cyclo and summed feature 3 (C16:1 ω7c/C16:1 ω6c).
32617606	10	65	dep	level	941:945	arg1	%					953:953	95-96%	948:953	95-96%	948:953	The average nucleotide identity (ANI) between strain H1T and the closely related Deinococcus members was below the cut-off level (95-96%) for species identification.
34157328	8	0	theme	DHFR	1367:1370	arg1	inhibitors					1307:1316	possible inhibitors	1298:1316	possible inhibitors of β-lactamase (Binding score: -7.936 kcal/mol), DHFR (Binding score: -5.691 kcal/mol) and FabI (Binding score: -8.673 kcal/mol)	1298:1445	In silico findings suggested CNC doped ZnO nanocomposites as possible inhibitors of β-lactamase (Binding score: -7.936 kcal/mol), DHFR (Binding score: -5.691 kcal/mol) and FabI (Binding score: -8.673 kcal/mol).
34157328	4	1	theme	photocatalytic	575:588	arg1	performance					590:600	Highly efficient photocatalytic performance	558:600	Highly efficient photocatalytic performance	558:600	Highly efficient photocatalytic performance was observed in basic medium (98%) relative to neutral (65%), and acidic (83%) was observed upon Mg and CNC co-doping.
34157328	0	2	theme	nanoparticles	80:92	arg1	antibacterial					17:29	antibacterial	17:29	antibacterial	17:29	Dye degradation, antibacterial and in-silico analysis of Mg/cellulose-doped ZnO nanoparticles.
34157328	0	2	theme	nanoparticles	80:92	arg1	analysis					45:52	in-silico analysis	35:52	in-silico analysis	35:52	Dye degradation, antibacterial and in-silico analysis of Mg/cellulose-doped ZnO nanoparticles.
34157328	0	2	theme	nanoparticles	80:92	arg1	degradation					4:14	degradation	4:14	degradation	4:14	Dye degradation, antibacterial and in-silico analysis of Mg/cellulose-doped ZnO nanoparticles.
34157328	5	3	theme	Significant	721:731	arg1	activity					746:753	Significant bactericidal activity	721:753	Significant bactericidal activity of doped ZnO nanoparticles	721:780	Significant bactericidal activity of doped ZnO nanoparticles depicted inhibition zones for G -ve and +ve bacteria ranging (2.20 - 4.25 mm) and (5.80-7.25 mm) for E. coli and (1.05 - 2.75 mm) and (2.80 - 4.75 mm) for S. aureus at low and high doses, respectively.
34157328	8	4	theme	Binding	1373:1379	arg1	score					1381:1385	Binding score	1373:1385	Binding score: -5.691 kcal/mol	1373:1402	In silico findings suggested CNC doped ZnO nanocomposites as possible inhibitors of β-lactamase (Binding score: -7.936 kcal/mol), DHFR (Binding score: -5.691 kcal/mol) and FabI (Binding score: -8.673 kcal/mol).
34157328	4	5	theme	efficient	565:573	arg1	performance					590:600	Highly efficient photocatalytic performance	558:600	Highly efficient photocatalytic performance	558:600	Highly efficient photocatalytic performance was observed in basic medium (98%) relative to neutral (65%), and acidic (83%) was observed upon Mg and CNC co-doping.
34157328	6	6	theme	G	1074:1074	arg1	+ve					1076:1078	G +ve	1074:1078	G +ve relative to G -ve	1074:1096	Overall, doped nanostructures showed significant (P < 0.05) bactericidal efficacy against G +ve relative to G -ve.
34157328	6	7	theme	bactericidal	1044:1055	arg1	efficacy					1057:1064	significant (P < 0.05) bactericidal efficacy	1021:1064	significant (P < 0.05) bactericidal efficacy against G +ve relative to G -ve	1021:1096	Overall, doped nanostructures showed significant (P < 0.05) bactericidal efficacy against G +ve relative to G -ve.
34157328	6	8	theme	significant	1021:1031	arg1	efficacy					1057:1064	significant (P < 0.05) bactericidal efficacy	1021:1064	significant (P < 0.05) bactericidal efficacy against G +ve relative to G -ve	1021:1096	Overall, doped nanostructures showed significant (P < 0.05) bactericidal efficacy against G +ve relative to G -ve.
34157328	2	9	theme	dye	276:278	arg1	degradation					280:290	dye degradation	276:290	dye degradation of methylene blue (MB)	276:313	The aim of present study is to remove dye degradation of methylene blue (MB) and bactericidal behavior with synthesized product.
34157328	7	10	theme	docking	1126:1132	arg1	studies					1134:1140	the molecular docking studies	1112:1140	the molecular docking studies	1112:1140	Furthermore, the molecular docking studies were employed to rationalize possible mechanism behind these in vitro bactericidal activities.
34157328	5	11	theme	high	958:961	arg1	doses					963:967	low and high doses	950:967	low and high doses	950:967	Significant bactericidal activity of doped ZnO nanoparticles depicted inhibition zones for G -ve and +ve bacteria ranging (2.20 - 4.25 mm) and (5.80-7.25 mm) for E. coli and (1.05 - 2.75 mm) and (2.80 - 4.75 mm) for S. aureus at low and high doses, respectively.
34157328	7	12	theme	possible	1171:1178	arg1	mechanism					1180:1188	possible mechanism	1171:1188	possible mechanism behind these in vitro bactericidal activities	1171:1234	Furthermore, the molecular docking studies were employed to rationalize possible mechanism behind these in vitro bactericidal activities.
34157328	3	13	theme	UV-Vis	516:521	arg1	spectrophotometer					523:539	UV-Vis spectrophotometer	516:539	UV-Vis spectrophotometer	516:539	Phase constitution, functional group analysis, optical behavior, elemental composition, morphology and microstructure were examined using XRD, FTIR, UV-Vis spectrophotometer, EDS and HR-TEM.
34157328	3	14	theme	optical	414:420	arg1	behavior					422:429	optical behavior	414:429	optical behavior	414:429	Phase constitution, functional group analysis, optical behavior, elemental composition, morphology and microstructure were examined using XRD, FTIR, UV-Vis spectrophotometer, EDS and HR-TEM.
34157328	5	15	theme	bactericidal	733:744	arg1	activity					746:753	Significant bactericidal activity	721:753	Significant bactericidal activity of doped ZnO nanoparticles	721:780	Significant bactericidal activity of doped ZnO nanoparticles depicted inhibition zones for G -ve and +ve bacteria ranging (2.20 - 4.25 mm) and (5.80-7.25 mm) for E. coli and (1.05 - 2.75 mm) and (2.80 - 4.75 mm) for S. aureus at low and high doses, respectively.
34157328	8	16	dep	DHFR	1367:1370	arg1	score					1381:1385	Binding score	1373:1385	Binding score: -5.691 kcal/mol	1373:1402	In silico findings suggested CNC doped ZnO nanocomposites as possible inhibitors of β-lactamase (Binding score: -7.936 kcal/mol), DHFR (Binding score: -5.691 kcal/mol) and FabI (Binding score: -8.673 kcal/mol).
34157328	8	17	theme	doped	1270:1274	arg1	nanocomposites					1280:1293	CNC doped ZnO nanocomposites	1266:1293	CNC doped ZnO nanocomposites as possible inhibitors of β-lactamase (Binding score: -7.936 kcal/mol), DHFR (Binding score: -5.691 kcal/mol) and FabI (Binding score: -8.673 kcal/mol)	1266:1445	In silico findings suggested CNC doped ZnO nanocomposites as possible inhibitors of β-lactamase (Binding score: -7.936 kcal/mol), DHFR (Binding score: -5.691 kcal/mol) and FabI (Binding score: -8.673 kcal/mol).
34157328	1	18	theme	-doped	173:178	arg1	ZnO					180:182	Mg into fixed amount of cellulose nanocrystals (CNC)-doped ZnO	121:182	Mg into fixed amount of cellulose nanocrystals (CNC)-doped ZnO	121:182	Various concentrations of Mg into fixed amount of cellulose nanocrystals (CNC)-doped ZnO were synthesized using facile chemical precipitation.
34157328	4	19	theme	relative	637:644	arg1	%					634:634	98%	632:634	98%	632:634	Highly efficient photocatalytic performance was observed in basic medium (98%) relative to neutral (65%), and acidic (83%) was observed upon Mg and CNC co-doping.
34157328	4	19	theme	relative	637:644	arg1	medium					624:629	basic medium	618:629	basic medium (98%) relative to neutral (65%)	618:661	Highly efficient photocatalytic performance was observed in basic medium (98%) relative to neutral (65%), and acidic (83%) was observed upon Mg and CNC co-doping.
34157328	1	20	theme	Various	95:101	arg1	concentrations					103:116	Various concentrations	95:116	Various concentrations of Mg into fixed amount of cellulose nanocrystals (CNC)-doped ZnO	95:182	Various concentrations of Mg into fixed amount of cellulose nanocrystals (CNC)-doped ZnO were synthesized using facile chemical precipitation.
34157328	1	21	theme	ZnO	180:182	arg1	concentrations					103:116	Various concentrations	95:116	Various concentrations of Mg into fixed amount of cellulose nanocrystals (CNC)-doped ZnO	95:182	Various concentrations of Mg into fixed amount of cellulose nanocrystals (CNC)-doped ZnO were synthesized using facile chemical precipitation.
34157328	7	22	dep	in	1203:1204	arg1	vitro					1206:1210	vitro	1206:1210	vitro	1206:1210	Furthermore, the molecular docking studies were employed to rationalize possible mechanism behind these in vitro bactericidal activities.
34157328	2	23	theme	blue	305:308	arg1	methylene					295:303	methylene blue	295:308	methylene blue (MB)	295:313	The aim of present study is to remove dye degradation of methylene blue (MB) and bactericidal behavior with synthesized product.
34157328	2	23	theme	blue	305:308	arg1	MB					311:312	MB	311:312	MB	311:312	The aim of present study is to remove dye degradation of methylene blue (MB) and bactericidal behavior with synthesized product.
34157328	8	24	theme	β-lactamase	1321:1331	arg1	inhibitors					1307:1316	possible inhibitors	1298:1316	possible inhibitors of β-lactamase (Binding score: -7.936 kcal/mol), DHFR (Binding score: -5.691 kcal/mol) and FabI (Binding score: -8.673 kcal/mol)	1298:1445	In silico findings suggested CNC doped ZnO nanocomposites as possible inhibitors of β-lactamase (Binding score: -7.936 kcal/mol), DHFR (Binding score: -5.691 kcal/mol) and FabI (Binding score: -8.673 kcal/mol).
34157328	5	25	theme	doped	758:762	arg1	nanoparticles					768:780	doped ZnO nanoparticles	758:780	doped ZnO nanoparticles	758:780	Significant bactericidal activity of doped ZnO nanoparticles depicted inhibition zones for G -ve and +ve bacteria ranging (2.20 - 4.25 mm) and (5.80-7.25 mm) for E. coli and (1.05 - 2.75 mm) and (2.80 - 4.75 mm) for S. aureus at low and high doses, respectively.
34157328	8	26	theme	FabI	1409:1412	arg1	inhibitors					1307:1316	possible inhibitors	1298:1316	possible inhibitors of β-lactamase (Binding score: -7.936 kcal/mol), DHFR (Binding score: -5.691 kcal/mol) and FabI (Binding score: -8.673 kcal/mol)	1298:1445	In silico findings suggested CNC doped ZnO nanocomposites as possible inhibitors of β-lactamase (Binding score: -7.936 kcal/mol), DHFR (Binding score: -5.691 kcal/mol) and FabI (Binding score: -8.673 kcal/mol).
34157328	8	27	theme	Binding	1334:1340	arg1	score					1342:1346	Binding score	1334:1346	Binding score: -7.936 kcal/mol	1334:1363	In silico findings suggested CNC doped ZnO nanocomposites as possible inhibitors of β-lactamase (Binding score: -7.936 kcal/mol), DHFR (Binding score: -5.691 kcal/mol) and FabI (Binding score: -8.673 kcal/mol).
34157328	6	28	theme	relative	1080:1087	arg1	+ve					1076:1078	G +ve	1074:1078	G +ve relative to G -ve	1074:1096	Overall, doped nanostructures showed significant (P < 0.05) bactericidal efficacy against G +ve relative to G -ve.
34157328	2	29	theme	methylene	295:303	arg1	degradation					280:290	dye degradation	276:290	dye degradation of methylene blue (MB)	276:313	The aim of present study is to remove dye degradation of methylene blue (MB) and bactericidal behavior with synthesized product.
34157328	2	29	theme	methylene	295:303	arg1	behavior					332:339	bactericidal behavior	319:339	bactericidal behavior with synthesized product	319:364	The aim of present study is to remove dye degradation of methylene blue (MB) and bactericidal behavior with synthesized product.
34157328	7	30	dep	mechanism	1180:1188	arg1	behind					1190:1195	behind	1190:1195	behind these in vitro bactericidal activities	1190:1234	Furthermore, the molecular docking studies were employed to rationalize possible mechanism behind these in vitro bactericidal activities.
34157328	5	31	theme	nanoparticles	768:780	arg1	activity					746:753	Significant bactericidal activity	721:753	Significant bactericidal activity of doped ZnO nanoparticles	721:780	Significant bactericidal activity of doped ZnO nanoparticles depicted inhibition zones for G -ve and +ve bacteria ranging (2.20 - 4.25 mm) and (5.80-7.25 mm) for E. coli and (1.05 - 2.75 mm) and (2.80 - 4.75 mm) for S. aureus at low and high doses, respectively.
34157328	3	32	theme	Phase	367:371	arg1	constitution					373:384	Phase constitution	367:384	Phase constitution	367:384	Phase constitution, functional group analysis, optical behavior, elemental composition, morphology and microstructure were examined using XRD, FTIR, UV-Vis spectrophotometer, EDS and HR-TEM.
34157328	4	33	theme	CNC	706:708	arg1	co-doping					710:718	CNC co-doping	706:718	CNC co-doping	706:718	Highly efficient photocatalytic performance was observed in basic medium (98%) relative to neutral (65%), and acidic (83%) was observed upon Mg and CNC co-doping.
34157328	6	34	theme	doped	993:997	arg1	nanostructures					999:1012	doped nanostructures	993:1012	doped nanostructures	993:1012	Overall, doped nanostructures showed significant (P < 0.05) bactericidal efficacy against G +ve relative to G -ve.
34157328	5	35	theme	+ve	822:824	arg1	bacteria					826:833	G -ve and +ve bacteria	812:833	G -ve and +ve bacteria ranging (2.20 - 4.25 mm) and (5.80-7.25 mm) for E. coli and (1.05 - 2.75 mm) and (2.80 - 4.75 mm) for S. aureus at low and high doses, respectively	812:981	Significant bactericidal activity of doped ZnO nanoparticles depicted inhibition zones for G -ve and +ve bacteria ranging (2.20 - 4.25 mm) and (5.80-7.25 mm) for E. coli and (1.05 - 2.75 mm) and (2.80 - 4.75 mm) for S. aureus at low and high doses, respectively.
34157328	0	36	theme	in-silico	35:43	arg1	analysis					45:52	in-silico analysis	35:52	in-silico analysis	35:52	Dye degradation, antibacterial and in-silico analysis of Mg/cellulose-doped ZnO nanoparticles.
34157328	8	37	theme	Binding	1415:1421	arg1	score					1423:1427	Binding score	1415:1427	Binding score: -8.673 kcal/mol	1415:1444	In silico findings suggested CNC doped ZnO nanocomposites as possible inhibitors of β-lactamase (Binding score: -7.936 kcal/mol), DHFR (Binding score: -5.691 kcal/mol) and FabI (Binding score: -8.673 kcal/mol).
34157328	5	38	theme	inhibition	791:800	arg1	zones					802:806	inhibition zones	791:806	inhibition zones for G -ve and +ve bacteria ranging (2.20 - 4.25 mm) and (5.80-7.25 mm) for E. coli and (1.05 - 2.75 mm) and (2.80 - 4.75 mm) for S. aureus at low and high doses, respectively	791:981	Significant bactericidal activity of doped ZnO nanoparticles depicted inhibition zones for G -ve and +ve bacteria ranging (2.20 - 4.25 mm) and (5.80-7.25 mm) for E. coli and (1.05 - 2.75 mm) and (2.80 - 4.75 mm) for S. aureus at low and high doses, respectively.
34157328	4	39	located	observed	606:613	arg1	%					634:634	98%	632:634	98%	632:634	Highly efficient photocatalytic performance was observed in basic medium (98%) relative to neutral (65%), and acidic (83%) was observed upon Mg and CNC co-doping.
34157328	4	39	located	observed	606:613	arg2	performance					590:600	Highly efficient photocatalytic performance	558:600	Highly efficient photocatalytic performance	558:600	Highly efficient photocatalytic performance was observed in basic medium (98%) relative to neutral (65%), and acidic (83%) was observed upon Mg and CNC co-doping.
34157328	4	39	located	observed	606:613	arg1	medium					624:629	basic medium	618:629	basic medium (98%) relative to neutral (65%)	618:661	Highly efficient photocatalytic performance was observed in basic medium (98%) relative to neutral (65%), and acidic (83%) was observed upon Mg and CNC co-doping.
34157328	8	40	dep	In	1237:1238	arg1	silico					1240:1245	silico	1240:1245	silico	1240:1245	In silico findings suggested CNC doped ZnO nanocomposites as possible inhibitors of β-lactamase (Binding score: -7.936 kcal/mol), DHFR (Binding score: -5.691 kcal/mol) and FabI (Binding score: -8.673 kcal/mol).
34157328	1	41	theme	facile	207:212	arg1	precipitation					223:235	facile chemical precipitation	207:235	facile chemical precipitation	207:235	Various concentrations of Mg into fixed amount of cellulose nanocrystals (CNC)-doped ZnO were synthesized using facile chemical precipitation.
34157328	8	42	theme	ZnO	1276:1278	arg1	nanocomposites					1280:1293	CNC doped ZnO nanocomposites	1266:1293	CNC doped ZnO nanocomposites as possible inhibitors of β-lactamase (Binding score: -7.936 kcal/mol), DHFR (Binding score: -5.691 kcal/mol) and FabI (Binding score: -8.673 kcal/mol)	1266:1445	In silico findings suggested CNC doped ZnO nanocomposites as possible inhibitors of β-lactamase (Binding score: -7.936 kcal/mol), DHFR (Binding score: -5.691 kcal/mol) and FabI (Binding score: -8.673 kcal/mol).
34157328	8	43	dep	score	1342:1346	arg1	-7.936 kcal/mol					1349:1363	-7.936 kcal/mol	1349:1363	Binding score: -7.936 kcal/mol	1334:1363	In silico findings suggested CNC doped ZnO nanocomposites as possible inhibitors of β-lactamase (Binding score: -7.936 kcal/mol), DHFR (Binding score: -5.691 kcal/mol) and FabI (Binding score: -8.673 kcal/mol).
34157328	5	44	theme	-ve	814:816	arg1	bacteria					826:833	G -ve and +ve bacteria	812:833	G -ve and +ve bacteria ranging (2.20 - 4.25 mm) and (5.80-7.25 mm) for E. coli and (1.05 - 2.75 mm) and (2.80 - 4.75 mm) for S. aureus at low and high doses, respectively	812:981	Significant bactericidal activity of doped ZnO nanoparticles depicted inhibition zones for G -ve and +ve bacteria ranging (2.20 - 4.25 mm) and (5.80-7.25 mm) for E. coli and (1.05 - 2.75 mm) and (2.80 - 4.75 mm) for S. aureus at low and high doses, respectively.
34157328	6	45	theme	G	1092:1092	arg1	-ve					1094:1096	G -ve	1092:1096	G -ve	1092:1096	Overall, doped nanostructures showed significant (P < 0.05) bactericidal efficacy against G +ve relative to G -ve.
34157328	1	46	theme	chemical	214:221	arg1	precipitation					223:235	facile chemical precipitation	207:235	facile chemical precipitation	207:235	Various concentrations of Mg into fixed amount of cellulose nanocrystals (CNC)-doped ZnO were synthesized using facile chemical precipitation.
34157328	3	47	theme	functional	387:396	arg1	analysis					404:411	functional group analysis	387:411	functional group analysis	387:411	Phase constitution, functional group analysis, optical behavior, elemental composition, morphology and microstructure were examined using XRD, FTIR, UV-Vis spectrophotometer, EDS and HR-TEM.
34157328	5	48	theme	low	950:952	arg1	doses					963:967	low and high doses	950:967	low and high doses	950:967	Significant bactericidal activity of doped ZnO nanoparticles depicted inhibition zones for G -ve and +ve bacteria ranging (2.20 - 4.25 mm) and (5.80-7.25 mm) for E. coli and (1.05 - 2.75 mm) and (2.80 - 4.75 mm) for S. aureus at low and high doses, respectively.
34157328	8	49	theme	In	1237:1238	arg1	findings					1247:1254	In silico findings	1237:1254	In silico findings	1237:1254	In silico findings suggested CNC doped ZnO nanocomposites as possible inhibitors of β-lactamase (Binding score: -7.936 kcal/mol), DHFR (Binding score: -5.691 kcal/mol) and FabI (Binding score: -8.673 kcal/mol).
34157328	2	50	theme	bactericidal	319:330	arg1	behavior					332:339	bactericidal behavior	319:339	bactericidal behavior with synthesized product	319:364	The aim of present study is to remove dye degradation of methylene blue (MB) and bactericidal behavior with synthesized product.
34157328	3	51	theme	group	398:402	arg1	analysis					404:411	functional group analysis	387:411	functional group analysis	387:411	Phase constitution, functional group analysis, optical behavior, elemental composition, morphology and microstructure were examined using XRD, FTIR, UV-Vis spectrophotometer, EDS and HR-TEM.
34157328	8	52	dep	β-lactamase	1321:1331	arg1	score					1342:1346	Binding score	1334:1346	Binding score: -7.936 kcal/mol	1334:1363	In silico findings suggested CNC doped ZnO nanocomposites as possible inhibitors of β-lactamase (Binding score: -7.936 kcal/mol), DHFR (Binding score: -5.691 kcal/mol) and FabI (Binding score: -8.673 kcal/mol).
34157328	8	53	dep	score	1423:1427	arg1	-8.673 kcal/mol					1430:1444	-8.673 kcal/mol	1430:1444	Binding score: -8.673 kcal/mol	1415:1444	In silico findings suggested CNC doped ZnO nanocomposites as possible inhibitors of β-lactamase (Binding score: -7.936 kcal/mol), DHFR (Binding score: -5.691 kcal/mol) and FabI (Binding score: -8.673 kcal/mol).
34157328	1	54	dep	-doped	173:178	arg1	Mg					121:122	Mg	121:122	Mg into fixed amount of cellulose nanocrystals	121:166	Various concentrations of Mg into fixed amount of cellulose nanocrystals (CNC)-doped ZnO were synthesized using facile chemical precipitation.
34157328	7	55	theme	in	1203:1204	arg1	activities					1225:1234	these in vitro bactericidal activities	1197:1234	these in vitro bactericidal activities	1197:1234	Furthermore, the molecular docking studies were employed to rationalize possible mechanism behind these in vitro bactericidal activities.
34157328	1	56	theme	fixed	129:133	arg1	amount					135:140	fixed amount	129:140	fixed amount of cellulose nanocrystals	129:166	Various concentrations of Mg into fixed amount of cellulose nanocrystals (CNC)-doped ZnO were synthesized using facile chemical precipitation.
34157328	1	56	theme	fixed	129:133	arg1	nanocrystals					155:166	cellulose nanocrystals	145:166	cellulose nanocrystals	145:166	Various concentrations of Mg into fixed amount of cellulose nanocrystals (CNC)-doped ZnO were synthesized using facile chemical precipitation.
34157328	8	57	dep	score	1381:1385	arg1	-5.691 kcal/mol					1388:1402	-5.691 kcal/mol	1388:1402	Binding score: -5.691 kcal/mol	1373:1402	In silico findings suggested CNC doped ZnO nanocomposites as possible inhibitors of β-lactamase (Binding score: -7.936 kcal/mol), DHFR (Binding score: -5.691 kcal/mol) and FabI (Binding score: -8.673 kcal/mol).
34157328	7	58	theme	bactericidal	1212:1223	arg1	activities					1225:1234	these in vitro bactericidal activities	1197:1234	these in vitro bactericidal activities	1197:1234	Furthermore, the molecular docking studies were employed to rationalize possible mechanism behind these in vitro bactericidal activities.
34157328	2	59	with	behavior	332:339	arg1	product					358:364	synthesized product	346:364	synthesized product	346:364	The aim of present study is to remove dye degradation of methylene blue (MB) and bactericidal behavior with synthesized product.
34157328	8	60	theme	possible	1298:1305	arg1	inhibitors					1307:1316	possible inhibitors	1298:1316	possible inhibitors of β-lactamase (Binding score: -7.936 kcal/mol), DHFR (Binding score: -5.691 kcal/mol) and FabI (Binding score: -8.673 kcal/mol)	1298:1445	In silico findings suggested CNC doped ZnO nanocomposites as possible inhibitors of β-lactamase (Binding score: -7.936 kcal/mol), DHFR (Binding score: -5.691 kcal/mol) and FabI (Binding score: -8.673 kcal/mol).
34157328	8	61	theme	CNC	1266:1268	arg1	nanocomposites					1280:1293	CNC doped ZnO nanocomposites	1266:1293	CNC doped ZnO nanocomposites as possible inhibitors of β-lactamase (Binding score: -7.936 kcal/mol), DHFR (Binding score: -5.691 kcal/mol) and FabI (Binding score: -8.673 kcal/mol)	1266:1445	In silico findings suggested CNC doped ZnO nanocomposites as possible inhibitors of β-lactamase (Binding score: -7.936 kcal/mol), DHFR (Binding score: -5.691 kcal/mol) and FabI (Binding score: -8.673 kcal/mol).
34157328	7	62	theme	molecular	1116:1124	arg1	studies					1134:1140	the molecular docking studies	1112:1140	the molecular docking studies	1112:1140	Furthermore, the molecular docking studies were employed to rationalize possible mechanism behind these in vitro bactericidal activities.
34157328	2	63	theme	study	257:261	arg1	aim					242:244	The aim	238:244	The aim of present study	238:261	The aim of present study is to remove dye degradation of methylene blue (MB) and bactericidal behavior with synthesized product.
34157328	6	64	theme	P < 0.05	1034:1041	arg1	efficacy					1057:1064	significant (P < 0.05) bactericidal efficacy	1021:1064	significant (P < 0.05) bactericidal efficacy against G +ve relative to G -ve	1021:1096	Overall, doped nanostructures showed significant (P < 0.05) bactericidal efficacy against G +ve relative to G -ve.
34157328	1	65	theme	nanocrystals	155:166	arg1	amount					135:140	fixed amount	129:140	fixed amount of cellulose nanocrystals	129:166	Various concentrations of Mg into fixed amount of cellulose nanocrystals (CNC)-doped ZnO were synthesized using facile chemical precipitation.
34157328	1	65	theme	nanocrystals	155:166	arg1	nanocrystals					155:166	cellulose nanocrystals	145:166	cellulose nanocrystals	145:166	Various concentrations of Mg into fixed amount of cellulose nanocrystals (CNC)-doped ZnO were synthesized using facile chemical precipitation.
34157328	0	66	theme	ZnO	76:78	arg1	nanoparticles					80:92	Mg/cellulose-doped ZnO nanoparticles	57:92	Mg/cellulose-doped ZnO nanoparticles	57:92	Dye degradation, antibacterial and in-silico analysis of Mg/cellulose-doped ZnO nanoparticles.
34157328	8	67	dep	FabI	1409:1412	arg1	score					1423:1427	Binding score	1415:1427	Binding score: -8.673 kcal/mol	1415:1444	In silico findings suggested CNC doped ZnO nanocomposites as possible inhibitors of β-lactamase (Binding score: -7.936 kcal/mol), DHFR (Binding score: -5.691 kcal/mol) and FabI (Binding score: -8.673 kcal/mol).
34157328	2	68	theme	present	249:255	arg1	study					257:261	present study	249:261	present study	249:261	The aim of present study is to remove dye degradation of methylene blue (MB) and bactericidal behavior with synthesized product.
34157328	1	69	theme	cellulose	145:153	arg1	nanocrystals					155:166	cellulose nanocrystals	145:166	cellulose nanocrystals	145:166	Various concentrations of Mg into fixed amount of cellulose nanocrystals (CNC)-doped ZnO were synthesized using facile chemical precipitation.
34157328	3	70	theme	elemental	432:440	arg1	composition					442:452	elemental composition	432:452	elemental composition	432:452	Phase constitution, functional group analysis, optical behavior, elemental composition, morphology and microstructure were examined using XRD, FTIR, UV-Vis spectrophotometer, EDS and HR-TEM.
34157328	2	71	with	degradation	280:290	arg1	product					358:364	synthesized product	346:364	synthesized product	346:364	The aim of present study is to remove dye degradation of methylene blue (MB) and bactericidal behavior with synthesized product.
34157328	0	72	theme	Mg/cellulose-doped	57:74	arg1	nanoparticles					80:92	Mg/cellulose-doped ZnO nanoparticles	57:92	Mg/cellulose-doped ZnO nanoparticles	57:92	Dye degradation, antibacterial and in-silico analysis of Mg/cellulose-doped ZnO nanoparticles.
34157328	5	73	theme	ZnO	764:766	arg1	nanoparticles					768:780	doped ZnO nanoparticles	758:780	doped ZnO nanoparticles	758:780	Significant bactericidal activity of doped ZnO nanoparticles depicted inhibition zones for G -ve and +ve bacteria ranging (2.20 - 4.25 mm) and (5.80-7.25 mm) for E. coli and (1.05 - 2.75 mm) and (2.80 - 4.75 mm) for S. aureus at low and high doses, respectively.
34157328	2	74	theme	synthesized	346:356	arg1	product					358:364	synthesized product	346:364	synthesized product	346:364	The aim of present study is to remove dye degradation of methylene blue (MB) and bactericidal behavior with synthesized product.
34157328	4	75	theme	basic	618:622	arg1	%					634:634	98%	632:634	98%	632:634	Highly efficient photocatalytic performance was observed in basic medium (98%) relative to neutral (65%), and acidic (83%) was observed upon Mg and CNC co-doping.
34157328	4	75	theme	basic	618:622	arg1	medium					624:629	basic medium	618:629	basic medium (98%) relative to neutral (65%)	618:661	Highly efficient photocatalytic performance was observed in basic medium (98%) relative to neutral (65%), and acidic (83%) was observed upon Mg and CNC co-doping.
32000259	4	0	from	effects	591:597	arg1	solution					658:665	HypoRP solution	651:665	HypoRP solution	651:665	METHODS Using porcine swine mesenteric arteries, the effects of up to 6-day incubation in a saline (PBS), UW, or HypoRP solution on the structure, cell viability, metabolism, and function were determined.
32000259	4	0	from	effects	591:597	arg1	viability					690:698	cell viability	685:698	cell viability	685:698	METHODS Using porcine swine mesenteric arteries, the effects of up to 6-day incubation in a saline (PBS), UW, or HypoRP solution on the structure, cell viability, metabolism, and function were determined.
32000259	4	0	from	effects	591:597	arg1	metabolism					701:710	metabolism	701:710	metabolism	701:710	METHODS Using porcine swine mesenteric arteries, the effects of up to 6-day incubation in a saline (PBS), UW, or HypoRP solution on the structure, cell viability, metabolism, and function were determined.
32000259	4	0	from	effects	591:597	arg1	UW					644:645	UW	644:645	UW	644:645	METHODS Using porcine swine mesenteric arteries, the effects of up to 6-day incubation in a saline (PBS), UW, or HypoRP solution on the structure, cell viability, metabolism, and function were determined.
32000259	4	0	from	effects	591:597	arg1	structure					674:682	the structure	670:682	the structure	670:682	METHODS Using porcine swine mesenteric arteries, the effects of up to 6-day incubation in a saline (PBS), UW, or HypoRP solution on the structure, cell viability, metabolism, and function were determined.
32000259	4	0	from	effects	591:597	arg1	saline					630:635	a saline	628:635	a saline (PBS)	628:641	METHODS Using porcine swine mesenteric arteries, the effects of up to 6-day incubation in a saline (PBS), UW, or HypoRP solution on the structure, cell viability, metabolism, and function were determined.
32000259	4	0	from	effects	591:597	arg1	function					717:724	function	717:724	function	717:724	METHODS Using porcine swine mesenteric arteries, the effects of up to 6-day incubation in a saline (PBS), UW, or HypoRP solution on the structure, cell viability, metabolism, and function were determined.
32000259	4	0	from	effects	591:597	arg1	PBS					638:640	PBS	638:640	PBS	638:640	METHODS Using porcine swine mesenteric arteries, the effects of up to 6-day incubation in a saline (PBS), UW, or HypoRP solution on the structure, cell viability, metabolism, and function were determined.
32000259	10	1	dep	CONCLUSIONS	1570:1580	arg1	improved					1602:1609	improved	1602:1609	improved long-term cold storage of porcine arteries by limiting structural alterations, including the collagen matrix, reducing apoptosis, and maintaining artery contraction-relaxation functions for up to 6 days	1602:1812	CONCLUSIONS The HypoRP solution improved long-term cold storage of porcine arteries by limiting structural alterations, including the collagen matrix, reducing apoptosis, and maintaining artery contraction-relaxation functions for up to 6 days.
32000259	8	2	theme	artery	1400:1405	arg1	rings					1407:1411	artery rings	1400:1411	artery rings stored in PBS and UW solution	1400:1441	Functional responsiveness to phenylephrine (PE) and sodium nitroprusside (SNP) decreased over time for artery rings stored in PBS and UW solution but not for those stored in HypoRP solution.
32000259	3	3	theme	arteries	496:503	arg1	survival					466:473	survival	466:473	survival	466:473	Here we determined whether the new hypothermic resuscitation and preservation solution HypoRP improves both structure, survival, and function of pig arteries during storage for up to 6 days.
32000259	3	3	theme	arteries	496:503	arg1	function					480:487	function	480:487	function	480:487	Here we determined whether the new hypothermic resuscitation and preservation solution HypoRP improves both structure, survival, and function of pig arteries during storage for up to 6 days.
32000259	3	3	theme	arteries	496:503	arg1	structure					455:463	structure	455:463	structure	455:463	Here we determined whether the new hypothermic resuscitation and preservation solution HypoRP improves both structure, survival, and function of pig arteries during storage for up to 6 days.
32000259	4	4	from	UW	644:645	arg1	effects					591:597	the effects	587:597	the effects of up to 6-day incubation in a saline (PBS), UW, or HypoRP solution on the structure, cell viability, metabolism, and function	587:724	METHODS Using porcine swine mesenteric arteries, the effects of up to 6-day incubation in a saline (PBS), UW, or HypoRP solution on the structure, cell viability, metabolism, and function were determined.
32000259	4	5	theme	mesenteric	566:575	arg1	arteries					577:584	porcine swine mesenteric arteries	552:584	porcine swine mesenteric arteries	552:584	METHODS Using porcine swine mesenteric arteries, the effects of up to 6-day incubation in a saline (PBS), UW, or HypoRP solution on the structure, cell viability, metabolism, and function were determined.
32000259	7	6	from	incubation	1115:1124	arg1	PBS					1129:1131	PBS	1129:1131	PBS	1129:1131	Additionally, while incubation in PBS led to a significant decrease in the metabolic activity, UW and HypoRP solutions allowed a stable to increased metabolic activity following 6 days of cold storage.
32000259	9	7	from	solution	1533:1540	arg1	cold-stored					1511:1521	cold-stored	1511:1521	cold-stored	1511:1521	Moreover, artery rings cold-stored in HypoRP solution were more sensitive to ATP.
32000259	4	8	theme	swine	560:564	arg1	arteries					577:584	porcine swine mesenteric arteries	552:584	porcine swine mesenteric arteries	552:584	METHODS Using porcine swine mesenteric arteries, the effects of up to 6-day incubation in a saline (PBS), UW, or HypoRP solution on the structure, cell viability, metabolism, and function were determined.
32000259	7	9	theme	increased	1234:1242	arg1	activity					1254:1261	increased metabolic activity	1234:1261	increased metabolic activity	1234:1261	Additionally, while incubation in PBS led to a significant decrease in the metabolic activity, UW and HypoRP solutions allowed a stable to increased metabolic activity following 6 days of cold storage.
32000259	7	10	theme	metabolic	1244:1252	arg1	activity					1254:1261	increased metabolic activity	1234:1261	increased metabolic activity	1234:1261	Additionally, while incubation in PBS led to a significant decrease in the metabolic activity, UW and HypoRP solutions allowed a stable to increased metabolic activity following 6 days of cold storage.
32000259	5	11	theme	tunica	870:875	arg1	media					877:881	the tunica media	866:881	the tunica media	866:881	RESULTS After incubation at 4°C, for up to 6 days, the structures of the arteries were significantly disrupted, especially the tunica media, following incubation in PBS, in contrast with incubation in the HypoRP solution and to a lesser extent, in UW solution.
32000259	10	12	theme	arteries	1645:1652	arg1	storage					1626:1632	long-term cold storage	1611:1632	long-term cold storage of porcine arteries	1611:1652	CONCLUSIONS The HypoRP solution improved long-term cold storage of porcine arteries by limiting structural alterations, including the collagen matrix, reducing apoptosis, and maintaining artery contraction-relaxation functions for up to 6 days.
32000259	2	13	theme	significant	306:316	arg1	4-6 h					339:343	4-6 h	339:343	4-6 h	339:343	The University of Wisconsin (UW) solution has demonstrated significant short-term benefits (4-6 h).
32000259	2	13	theme	significant	306:316	arg1	benefits					329:336	significant short-term benefits	306:336	significant short-term benefits (4-6 h)	306:344	The University of Wisconsin (UW) solution has demonstrated significant short-term benefits (4-6 h).
32000259	9	14	theme	cold-stored	1511:1521	arg1	rings					1505:1509	artery rings	1498:1509	artery rings cold-stored in HypoRP solution	1498:1540	Moreover, artery rings cold-stored in HypoRP solution were more sensitive to ATP.
32000259	2	15	theme	UW	276:277	arg1	solution					280:287	Wisconsin (UW) solution	265:287	Wisconsin (UW) solution	265:287	The University of Wisconsin (UW) solution has demonstrated significant short-term benefits (4-6 h).
32000259	5	16	dep	media	877:881	arg1	solution					994:1001	UW solution	991:1001	UW solution	991:1001	RESULTS After incubation at 4°C, for up to 6 days, the structures of the arteries were significantly disrupted, especially the tunica media, following incubation in PBS, in contrast with incubation in the HypoRP solution and to a lesser extent, in UW solution.
32000259	0	17	theme	HypoRP	84:89	arg1	Solution					104:111	the HypoRP Cold-storage Solution	80:111	the HypoRP Cold-storage Solution	80:111	Structure and Function of Porcine Arteries Are Preserved for up to 6 Days Using the HypoRP Cold-storage Solution.
32000259	1	18	theme	vascularized	179:190	arg1	VCAs					214:217	VCAs	214:217	VCAs	214:217	BACKGROUND Maintaining functional vessels during preservation of vascularized composite allografts (VCAs) remains a major challenge.
32000259	1	18	theme	vascularized	179:190	arg1	allografts					202:211	vascularized composite allografts	179:211	vascularized composite allografts (VCAs)	179:218	BACKGROUND Maintaining functional vessels during preservation of vascularized composite allografts (VCAs) remains a major challenge.
32000259	5	19	with	contrast	916:923	arg1	incubation					930:939	incubation	930:939	incubation in the HypoRP solution	930:962	RESULTS After incubation at 4°C, for up to 6 days, the structures of the arteries were significantly disrupted, especially the tunica media, following incubation in PBS, in contrast with incubation in the HypoRP solution and to a lesser extent, in UW solution.
32000259	9	20	theme	HypoRP	1526:1531	arg1	solution					1533:1540	HypoRP solution	1526:1540	HypoRP solution	1526:1540	Moreover, artery rings cold-stored in HypoRP solution were more sensitive to ATP.
32000259	2	21	theme	Wisconsin	265:273	arg1	solution					280:287	Wisconsin (UW) solution	265:287	Wisconsin (UW) solution	265:287	The University of Wisconsin (UW) solution has demonstrated significant short-term benefits (4-6 h).
32000259	4	22	from	saline	630:635	arg1	effects					591:597	the effects	587:597	the effects of up to 6-day incubation in a saline (PBS), UW, or HypoRP solution on the structure, cell viability, metabolism, and function	587:724	METHODS Using porcine swine mesenteric arteries, the effects of up to 6-day incubation in a saline (PBS), UW, or HypoRP solution on the structure, cell viability, metabolism, and function were determined.
32000259	7	23	theme	cold	1283:1286	arg1	storage					1288:1294	cold storage	1283:1294	cold storage	1283:1294	Additionally, while incubation in PBS led to a significant decrease in the metabolic activity, UW and HypoRP solutions allowed a stable to increased metabolic activity following 6 days of cold storage.
32000259	10	24	theme	cold	1621:1624	arg1	storage					1626:1632	long-term cold storage	1611:1632	long-term cold storage of porcine arteries	1611:1652	CONCLUSIONS The HypoRP solution improved long-term cold storage of porcine arteries by limiting structural alterations, including the collagen matrix, reducing apoptosis, and maintaining artery contraction-relaxation functions for up to 6 days.
32000259	5	25	theme	UW	991:992	arg1	solution					994:1001	UW solution	991:1001	UW solution	991:1001	RESULTS After incubation at 4°C, for up to 6 days, the structures of the arteries were significantly disrupted, especially the tunica media, following incubation in PBS, in contrast with incubation in the HypoRP solution and to a lesser extent, in UW solution.
32000259	9	26	theme	artery	1498:1503	arg1	rings					1505:1509	artery rings	1498:1509	artery rings cold-stored in HypoRP solution	1498:1540	Moreover, artery rings cold-stored in HypoRP solution were more sensitive to ATP.
32000259	7	27	from	decrease	1154:1161	arg1	activity					1180:1187	the metabolic activity	1166:1187	the metabolic activity	1166:1187	Additionally, while incubation in PBS led to a significant decrease in the metabolic activity, UW and HypoRP solutions allowed a stable to increased metabolic activity following 6 days of cold storage.
32000259	0	28	dep	6	67:67	arg1	to					64:65	to	64:65	to	64:65	Structure and Function of Porcine Arteries Are Preserved for up to 6 Days Using the HypoRP Cold-storage Solution.
32000259	10	29	dep	6	1807:1807	arg1	to					1804:1805	to	1804:1805	to	1804:1805	CONCLUSIONS The HypoRP solution improved long-term cold storage of porcine arteries by limiting structural alterations, including the collagen matrix, reducing apoptosis, and maintaining artery contraction-relaxation functions for up to 6 days.
32000259	3	30	theme	solution	425:432	arg1	HypoRP					434:439	preservation solution HypoRP	412:439	preservation solution HypoRP	412:439	Here we determined whether the new hypothermic resuscitation and preservation solution HypoRP improves both structure, survival, and function of pig arteries during storage for up to 6 days.
32000259	3	31	theme	new	378:380	arg1	resuscitation					394:406	the new hypothermic resuscitation	374:406	the new hypothermic resuscitation	374:406	Here we determined whether the new hypothermic resuscitation and preservation solution HypoRP improves both structure, survival, and function of pig arteries during storage for up to 6 days.
32000259	8	32	theme	PBS	1423:1425	arg1	solution					1434:1441	PBS and UW solution	1423:1441	PBS and UW solution	1423:1441	Functional responsiveness to phenylephrine (PE) and sodium nitroprusside (SNP) decreased over time for artery rings stored in PBS and UW solution but not for those stored in HypoRP solution.
32000259	7	33	theme	storage	1288:1294	arg1	days					1275:1278	6 days	1273:1278	6 days of cold storage	1273:1294	Additionally, while incubation in PBS led to a significant decrease in the metabolic activity, UW and HypoRP solutions allowed a stable to increased metabolic activity following 6 days of cold storage.
32000259	1	34	theme	composite	192:200	arg1	VCAs					214:217	VCAs	214:217	VCAs	214:217	BACKGROUND Maintaining functional vessels during preservation of vascularized composite allografts (VCAs) remains a major challenge.
32000259	1	34	theme	composite	192:200	arg1	allografts					202:211	vascularized composite allografts	179:211	vascularized composite allografts (VCAs)	179:218	BACKGROUND Maintaining functional vessels during preservation of vascularized composite allografts (VCAs) remains a major challenge.
32000259	4	35	theme	cell	685:688	arg1	viability					690:698	cell viability	685:698	cell viability	685:698	METHODS Using porcine swine mesenteric arteries, the effects of up to 6-day incubation in a saline (PBS), UW, or HypoRP solution on the structure, cell viability, metabolism, and function were determined.
32000259	4	36	from	incubation	614:623	arg1	UW					644:645	UW	644:645	UW	644:645	METHODS Using porcine swine mesenteric arteries, the effects of up to 6-day incubation in a saline (PBS), UW, or HypoRP solution on the structure, cell viability, metabolism, and function were determined.
32000259	4	36	from	incubation	614:623	arg1	saline					630:635	a saline	628:635	a saline (PBS)	628:641	METHODS Using porcine swine mesenteric arteries, the effects of up to 6-day incubation in a saline (PBS), UW, or HypoRP solution on the structure, cell viability, metabolism, and function were determined.
32000259	4	36	from	incubation	614:623	arg1	solution					658:665	HypoRP solution	651:665	HypoRP solution	651:665	METHODS Using porcine swine mesenteric arteries, the effects of up to 6-day incubation in a saline (PBS), UW, or HypoRP solution on the structure, cell viability, metabolism, and function were determined.
32000259	4	36	from	incubation	614:623	arg1	PBS					638:640	PBS	638:640	PBS	638:640	METHODS Using porcine swine mesenteric arteries, the effects of up to 6-day incubation in a saline (PBS), UW, or HypoRP solution on the structure, cell viability, metabolism, and function were determined.
32000259	1	37	theme	allografts	202:211	arg1	preservation					163:174	preservation	163:174	preservation of vascularized composite allografts (VCAs)	163:218	BACKGROUND Maintaining functional vessels during preservation of vascularized composite allografts (VCAs) remains a major challenge.
32000259	10	38	theme	porcine	1637:1643	arg1	arteries					1645:1652	porcine arteries	1637:1652	porcine arteries	1637:1652	CONCLUSIONS The HypoRP solution improved long-term cold storage of porcine arteries by limiting structural alterations, including the collagen matrix, reducing apoptosis, and maintaining artery contraction-relaxation functions for up to 6 days.
32000259	4	39	theme	porcine	552:558	arg1	arteries					577:584	porcine swine mesenteric arteries	552:584	porcine swine mesenteric arteries	552:584	METHODS Using porcine swine mesenteric arteries, the effects of up to 6-day incubation in a saline (PBS), UW, or HypoRP solution on the structure, cell viability, metabolism, and function were determined.
32000259	0	40	theme	Cold-storage	91:102	arg1	Solution					104:111	the HypoRP Cold-storage Solution	80:111	the HypoRP Cold-storage Solution	80:111	Structure and Function of Porcine Arteries Are Preserved for up to 6 Days Using the HypoRP Cold-storage Solution.
32000259	10	41	theme	long-term	1611:1619	arg1	storage					1626:1632	long-term cold storage	1611:1632	long-term cold storage of porcine arteries	1611:1652	CONCLUSIONS The HypoRP solution improved long-term cold storage of porcine arteries by limiting structural alterations, including the collagen matrix, reducing apoptosis, and maintaining artery contraction-relaxation functions for up to 6 days.
32000259	0	42	theme	Arteries	34:41	arg1	Function					14:21	Function	14:21	Function	14:21	Structure and Function of Porcine Arteries Are Preserved for up to 6 Days Using the HypoRP Cold-storage Solution.
32000259	0	42	theme	Arteries	34:41	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and Function of Porcine Arteries Are Preserved for up to 6 Days Using the HypoRP Cold-storage Solution.
32000259	5	43	theme	arteries	816:823	arg1	structures					798:807	the structures	794:807	the structures of the arteries	794:823	RESULTS After incubation at 4°C, for up to 6 days, the structures of the arteries were significantly disrupted, especially the tunica media, following incubation in PBS, in contrast with incubation in the HypoRP solution and to a lesser extent, in UW solution.
32000259	7	44	theme	metabolic	1170:1178	arg1	activity					1180:1187	the metabolic activity	1166:1187	the metabolic activity	1166:1187	Additionally, while incubation in PBS led to a significant decrease in the metabolic activity, UW and HypoRP solutions allowed a stable to increased metabolic activity following 6 days of cold storage.
32000259	5	45	dep	6	786:786	arg1	to					783:784	to	783:784	to	783:784	RESULTS After incubation at 4°C, for up to 6 days, the structures of the arteries were significantly disrupted, especially the tunica media, following incubation in PBS, in contrast with incubation in the HypoRP solution and to a lesser extent, in UW solution.
32000259	6	46	theme	increased	1043:1051	arg1	caspase					1060:1066	increased active caspase 3	1043:1068	increased active caspase 3 indicative of apoptosis	1043:1092	Those disruptions were associated with increased active caspase 3 indicative of apoptosis.
32000259	0	47	theme	Porcine	26:32	arg1	Arteries					34:41	Porcine Arteries	26:41	Porcine Arteries	26:41	Structure and Function of Porcine Arteries Are Preserved for up to 6 Days Using the HypoRP Cold-storage Solution.
32000259	3	48	theme	preservation	412:423	arg1	HypoRP					434:439	preservation solution HypoRP	412:439	preservation solution HypoRP	412:439	Here we determined whether the new hypothermic resuscitation and preservation solution HypoRP improves both structure, survival, and function of pig arteries during storage for up to 6 days.
32000259	10	49	theme	structural	1666:1675	arg1	alterations					1677:1687	structural alterations	1666:1687	structural alterations	1666:1687	CONCLUSIONS The HypoRP solution improved long-term cold storage of porcine arteries by limiting structural alterations, including the collagen matrix, reducing apoptosis, and maintaining artery contraction-relaxation functions for up to 6 days.
32000259	4	50	theme	incubation	614:623	arg1	effects					591:597	the effects	587:597	the effects of up to 6-day incubation in a saline (PBS), UW, or HypoRP solution on the structure, cell viability, metabolism, and function	587:724	METHODS Using porcine swine mesenteric arteries, the effects of up to 6-day incubation in a saline (PBS), UW, or HypoRP solution on the structure, cell viability, metabolism, and function were determined.
32000259	3	51	theme	hypothermic	382:392	arg1	resuscitation					394:406	the new hypothermic resuscitation	374:406	the new hypothermic resuscitation	374:406	Here we determined whether the new hypothermic resuscitation and preservation solution HypoRP improves both structure, survival, and function of pig arteries during storage for up to 6 days.
32000259	8	52	theme	UW	1431:1432	arg1	solution					1434:1441	PBS and UW solution	1423:1441	PBS and UW solution	1423:1441	Functional responsiveness to phenylephrine (PE) and sodium nitroprusside (SNP) decreased over time for artery rings stored in PBS and UW solution but not for those stored in HypoRP solution.
32000259	5	53	theme	lesser	973:978	arg1	extent					980:985	a lesser extent	971:985	a lesser extent	971:985	RESULTS After incubation at 4°C, for up to 6 days, the structures of the arteries were significantly disrupted, especially the tunica media, following incubation in PBS, in contrast with incubation in the HypoRP solution and to a lesser extent, in UW solution.
32000259	4	54	dep	6-day	608:612	arg1	to					605:606	to	605:606	to	605:606	METHODS Using porcine swine mesenteric arteries, the effects of up to 6-day incubation in a saline (PBS), UW, or HypoRP solution on the structure, cell viability, metabolism, and function were determined.
32000259	4	55	theme	HypoRP	651:656	arg1	solution					658:665	HypoRP solution	651:665	HypoRP solution	651:665	METHODS Using porcine swine mesenteric arteries, the effects of up to 6-day incubation in a saline (PBS), UW, or HypoRP solution on the structure, cell viability, metabolism, and function were determined.
32000259	10	56	theme	contraction-relaxation	1764:1785	arg1	functions					1787:1795	artery contraction-relaxation functions	1757:1795	artery contraction-relaxation functions for up to 6 days	1757:1812	CONCLUSIONS The HypoRP solution improved long-term cold storage of porcine arteries by limiting structural alterations, including the collagen matrix, reducing apoptosis, and maintaining artery contraction-relaxation functions for up to 6 days.
32000259	10	57	theme	collagen	1704:1711	arg1	matrix					1713:1718	the collagen matrix	1700:1718	the collagen matrix	1700:1718	CONCLUSIONS The HypoRP solution improved long-term cold storage of porcine arteries by limiting structural alterations, including the collagen matrix, reducing apoptosis, and maintaining artery contraction-relaxation functions for up to 6 days.
32000259	8	58	theme	sodium	1349:1354	arg1	SNP					1371:1373	SNP	1371:1373	SNP	1371:1373	Functional responsiveness to phenylephrine (PE) and sodium nitroprusside (SNP) decreased over time for artery rings stored in PBS and UW solution but not for those stored in HypoRP solution.
32000259	8	58	theme	sodium	1349:1354	arg1	nitroprusside					1356:1368	sodium nitroprusside	1349:1368	sodium nitroprusside (SNP)	1349:1374	Functional responsiveness to phenylephrine (PE) and sodium nitroprusside (SNP) decreased over time for artery rings stored in PBS and UW solution but not for those stored in HypoRP solution.
32000259	10	59	theme	artery	1757:1762	arg1	functions					1787:1795	artery contraction-relaxation functions	1757:1795	artery contraction-relaxation functions for up to 6 days	1757:1812	CONCLUSIONS The HypoRP solution improved long-term cold storage of porcine arteries by limiting structural alterations, including the collagen matrix, reducing apoptosis, and maintaining artery contraction-relaxation functions for up to 6 days.
32000259	3	60	theme	pig	492:494	arg1	arteries					496:503	pig arteries	492:503	pig arteries	492:503	Here we determined whether the new hypothermic resuscitation and preservation solution HypoRP improves both structure, survival, and function of pig arteries during storage for up to 6 days.
32000259	8	61	theme	HypoRP	1471:1476	arg1	solution					1478:1485	HypoRP solution	1471:1485	HypoRP solution	1471:1485	Functional responsiveness to phenylephrine (PE) and sodium nitroprusside (SNP) decreased over time for artery rings stored in PBS and UW solution but not for those stored in HypoRP solution.
32000259	5	62	from	incubation	930:939	arg1	solution					955:962	the HypoRP solution	944:962	the HypoRP solution	944:962	RESULTS After incubation at 4°C, for up to 6 days, the structures of the arteries were significantly disrupted, especially the tunica media, following incubation in PBS, in contrast with incubation in the HypoRP solution and to a lesser extent, in UW solution.
32000259	8	63	theme	Functional	1297:1306	arg1	responsiveness					1308:1321	Functional responsiveness	1297:1321	Functional responsiveness to phenylephrine (PE) and sodium nitroprusside (SNP)	1297:1374	Functional responsiveness to phenylephrine (PE) and sodium nitroprusside (SNP) decreased over time for artery rings stored in PBS and UW solution but not for those stored in HypoRP solution.
32000259	5	64	from	4°C	771:773	arg1	incubation					757:766	incubation	757:766	incubation at 4°C	757:773	RESULTS After incubation at 4°C, for up to 6 days, the structures of the arteries were significantly disrupted, especially the tunica media, following incubation in PBS, in contrast with incubation in the HypoRP solution and to a lesser extent, in UW solution.
32000259	3	65	dep	6	530:530	arg1	to					527:528	to	527:528	to	527:528	Here we determined whether the new hypothermic resuscitation and preservation solution HypoRP improves both structure, survival, and function of pig arteries during storage for up to 6 days.
32000259	4	66	from	solution	658:665	arg1	effects					591:597	the effects	587:597	the effects of up to 6-day incubation in a saline (PBS), UW, or HypoRP solution on the structure, cell viability, metabolism, and function	587:724	METHODS Using porcine swine mesenteric arteries, the effects of up to 6-day incubation in a saline (PBS), UW, or HypoRP solution on the structure, cell viability, metabolism, and function were determined.
32000259	6	67	theme	active	1053:1058	arg1	caspase					1060:1066	increased active caspase 3	1043:1068	increased active caspase 3 indicative of apoptosis	1043:1092	Those disruptions were associated with increased active caspase 3 indicative of apoptosis.
32000259	5	68	theme	HypoRP	948:953	arg1	solution					955:962	the HypoRP solution	944:962	the HypoRP solution	944:962	RESULTS After incubation at 4°C, for up to 6 days, the structures of the arteries were significantly disrupted, especially the tunica media, following incubation in PBS, in contrast with incubation in the HypoRP solution and to a lesser extent, in UW solution.
32000259	6	69	theme	indicative	1070:1079	arg1	caspase					1060:1066	increased active caspase 3	1043:1068	increased active caspase 3 indicative of apoptosis	1043:1092	Those disruptions were associated with increased active caspase 3 indicative of apoptosis.
32000259	2	70	theme	short-term	318:327	arg1	4-6 h					339:343	4-6 h	339:343	4-6 h	339:343	The University of Wisconsin (UW) solution has demonstrated significant short-term benefits (4-6 h).
32000259	2	70	theme	short-term	318:327	arg1	benefits					329:336	significant short-term benefits	306:336	significant short-term benefits (4-6 h)	306:344	The University of Wisconsin (UW) solution has demonstrated significant short-term benefits (4-6 h).
32000259	5	71	from	incubation	894:903	arg1	PBS					908:910	PBS	908:910	PBS	908:910	RESULTS After incubation at 4°C, for up to 6 days, the structures of the arteries were significantly disrupted, especially the tunica media, following incubation in PBS, in contrast with incubation in the HypoRP solution and to a lesser extent, in UW solution.
32000259	5	71	from	incubation	894:903	arg1	contrast					916:923	contrast	916:923	contrast with incubation in the HypoRP solution	916:962	RESULTS After incubation at 4°C, for up to 6 days, the structures of the arteries were significantly disrupted, especially the tunica media, following incubation in PBS, in contrast with incubation in the HypoRP solution and to a lesser extent, in UW solution.
32000259	6	72	theme	apoptosis	1084:1092	arg1	indicative					1070:1079	indicative	1070:1079	indicative	1070:1079	Those disruptions were associated with increased active caspase 3 indicative of apoptosis.
32000259	4	73	theme	6-day	608:612	arg1	incubation					614:623	up to 6-day incubation	602:623	up to 6-day incubation in a saline (PBS), UW, or HypoRP solution	602:665	METHODS Using porcine swine mesenteric arteries, the effects of up to 6-day incubation in a saline (PBS), UW, or HypoRP solution on the structure, cell viability, metabolism, and function were determined.
32000259	10	74	theme	HypoRP	1586:1591	arg1	solution					1593:1600	The HypoRP solution	1582:1600	The HypoRP solution	1582:1600	CONCLUSIONS The HypoRP solution improved long-term cold storage of porcine arteries by limiting structural alterations, including the collagen matrix, reducing apoptosis, and maintaining artery contraction-relaxation functions for up to 6 days.
32000259	1	75	theme	major	230:234	arg1	challenge					236:244	a major challenge	228:244	a major challenge	228:244	BACKGROUND Maintaining functional vessels during preservation of vascularized composite allografts (VCAs) remains a major challenge.
32000259	7	76	dep	UW	1190:1191	arg1	solutions					1204:1212	solutions	1204:1212	solutions	1204:1212	Additionally, while incubation in PBS led to a significant decrease in the metabolic activity, UW and HypoRP solutions allowed a stable to increased metabolic activity following 6 days of cold storage.
32000259	7	77	theme	significant	1142:1152	arg1	decrease					1154:1161	a significant decrease	1140:1161	a significant decrease in the metabolic activity	1140:1187	Additionally, while incubation in PBS led to a significant decrease in the metabolic activity, UW and HypoRP solutions allowed a stable to increased metabolic activity following 6 days of cold storage.
32000259	1	78	theme	functional	137:146	arg1	vessels					148:154	functional vessels	137:154	functional vessels	137:154	BACKGROUND Maintaining functional vessels during preservation of vascularized composite allografts (VCAs) remains a major challenge.
32000259	9	79	from	cold-stored	1511:1521	arg1	solution					1533:1540	HypoRP solution	1526:1540	HypoRP solution	1526:1540	Moreover, artery rings cold-stored in HypoRP solution were more sensitive to ATP.
33853047	2	0	theme	DOX	487:489	arg1	drug					479:482	the chemotherapeutic drug	458:482	the chemotherapeutic drug of DOX	458:489	Mesoporous structured UiO-66 MOFs were synthesized, and carboxymethylcellulose (CMC) was loaded for sensitive pH response and also as a linker to encapsulate the chemotherapeutic drug of DOX.
33853047	7	1	theme	higher	1083:1088	arg1	solubility					1094:1103	higher DOX solubility	1083:1103	higher DOX solubility	1083:1103	A lower pH was more favorable for DOX release due to the CMC shrinking and higher DOX solubility in an acidic environment.
33853047	7	2	theme	lower	1010:1014	arg1	favorable					1028:1036	favorable	1028:1036	favorable	1028:1036	A lower pH was more favorable for DOX release due to the CMC shrinking and higher DOX solubility in an acidic environment.
33853047	7	2	theme	lower	1010:1014	arg1	pH					1016:1017	A lower pH	1008:1017	A lower pH	1008:1017	A lower pH was more favorable for DOX release due to the CMC shrinking and higher DOX solubility in an acidic environment.
33853047	4	3	theme	MOFs	660:663	arg1	structure					617:625	structure	617:625	structure	617:625	The structure and crystalization of the UiO-66 MOFs were determined by the Transmitting Electron Microscope (TEM) and x-ray diffraction methods, while the loading of CMC and DOX was inspected by Fourier Transform InfraRed (FT-IR) and UV-vis spectroscopy.
33853047	4	3	theme	MOFs	660:663	arg1	crystalization					631:644	crystalization	631:644	crystalization	631:644	The structure and crystalization of the UiO-66 MOFs were determined by the Transmitting Electron Microscope (TEM) and x-ray diffraction methods, while the loading of CMC and DOX was inspected by Fourier Transform InfraRed (FT-IR) and UV-vis spectroscopy.
33853047	4	4	theme	UV-vis	847:852	arg1	spectroscopy					854:865	InfraRed (FT-IR) and UV-vis spectroscopy	826:865	InfraRed (FT-IR) and UV-vis spectroscopy	826:865	The structure and crystalization of the UiO-66 MOFs were determined by the Transmitting Electron Microscope (TEM) and x-ray diffraction methods, while the loading of CMC and DOX was inspected by Fourier Transform InfraRed (FT-IR) and UV-vis spectroscopy.
33853047	9	5	theme	delivery	1395:1402	arg1	carrier					1404:1410	an efficient drug delivery carrier	1377:1410	an efficient drug delivery carrier for chemotherapeutic drug and release	1377:1448	UiO-66 MOFs were demonstrated to be an efficient drug delivery carrier for chemotherapeutic drug and release.
33853047	9	5	theme	delivery	1395:1402	arg1	MOFs					1348:1351	UiO-66 MOFs	1341:1351	UiO-66 MOFs	1341:1351	UiO-66 MOFs were demonstrated to be an efficient drug delivery carrier for chemotherapeutic drug and release.
33853047	7	6	theme	acidic	1111:1116	arg1	environment					1118:1128	an acidic environment	1108:1128	an acidic environment	1108:1128	A lower pH was more favorable for DOX release due to the CMC shrinking and higher DOX solubility in an acidic environment.
33853047	4	7	dep	structure	617:625	arg1	The					613:615	The	613:615	The	613:615	The structure and crystalization of the UiO-66 MOFs were determined by the Transmitting Electron Microscope (TEM) and x-ray diffraction methods, while the loading of CMC and DOX was inspected by Fourier Transform InfraRed (FT-IR) and UV-vis spectroscopy.
33853047	0	8	theme	pH-responsive	96:108	arg1	delivery					115:122	efficient pH-responsive drug delivery	86:122	efficient pH-responsive drug delivery	86:122	Synthesis and surface modification of mesoporous metal-organic framework (UiO-66) for efficient pH-responsive drug delivery and lung cancer treatment.
33853047	5	9	theme	different	921:929	arg1	pH					931:932	different pH	921:932	different pH	921:932	The DOX release from UiO-66/CMC@DOX was tested under different pH at 37 °C.
33853047	4	10	theme	InfraRed	826:833	arg1	spectroscopy					854:865	InfraRed (FT-IR) and UV-vis spectroscopy	826:865	InfraRed (FT-IR) and UV-vis spectroscopy	826:865	The structure and crystalization of the UiO-66 MOFs were determined by the Transmitting Electron Microscope (TEM) and x-ray diffraction methods, while the loading of CMC and DOX was inspected by Fourier Transform InfraRed (FT-IR) and UV-vis spectroscopy.
33853047	5	11	theme	@	899:899	arg1	DOX					900:902	UiO-66/CMC@DOX	889:902	UiO-66/CMC@DOX	889:902	The DOX release from UiO-66/CMC@DOX was tested under different pH at 37 °C.
33853047	5	12	theme	UiO-66/CMC	889:898	arg1	DOX					900:902	UiO-66/CMC@DOX	889:902	UiO-66/CMC@DOX	889:902	The DOX release from UiO-66/CMC@DOX was tested under different pH at 37 °C.
33853047	0	13	theme	efficient	86:94	arg1	delivery					115:122	efficient pH-responsive drug delivery	86:122	efficient pH-responsive drug delivery	86:122	Synthesis and surface modification of mesoporous metal-organic framework (UiO-66) for efficient pH-responsive drug delivery and lung cancer treatment.
33853047	9	14	theme	efficient	1380:1388	arg1	carrier					1404:1410	an efficient drug delivery carrier	1377:1410	an efficient drug delivery carrier for chemotherapeutic drug and release	1377:1448	UiO-66 MOFs were demonstrated to be an efficient drug delivery carrier for chemotherapeutic drug and release.
33853047	9	14	theme	efficient	1380:1388	arg1	MOFs					1348:1351	UiO-66 MOFs	1341:1351	UiO-66 MOFs	1341:1351	UiO-66 MOFs were demonstrated to be an efficient drug delivery carrier for chemotherapeutic drug and release.
33853047	8	15	theme	ml-1	1205:1208	arg1	concentration					1184:1196	the DOX concentration	1176:1196	the DOX concentration of 4μg ml-1	1176:1208	The cytotoxicity study indicated that, under the DOX concentration of 4μg ml-1, the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC was 28%, which was lower than that from free DOX solution (47%).
33853047	8	16	theme	4μg	1201:1203	arg1	ml-1					1205:1208	4μg ml-1	1201:1208	4μg ml-1	1201:1208	The cytotoxicity study indicated that, under the DOX concentration of 4μg ml-1, the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC was 28%, which was lower than that from free DOX solution (47%).
33853047	4	17	theme	Transmitting	688:699	arg1	Microscope					710:719	the Transmitting Electron Microscope	684:719	the Transmitting Electron Microscope (TEM)	684:725	The structure and crystalization of the UiO-66 MOFs were determined by the Transmitting Electron Microscope (TEM) and x-ray diffraction methods, while the loading of CMC and DOX was inspected by Fourier Transform InfraRed (FT-IR) and UV-vis spectroscopy.
33853047	4	17	theme	Transmitting	688:699	arg1	TEM					722:724	TEM	722:724	TEM	722:724	The structure and crystalization of the UiO-66 MOFs were determined by the Transmitting Electron Microscope (TEM) and x-ray diffraction methods, while the loading of CMC and DOX was inspected by Fourier Transform InfraRed (FT-IR) and UV-vis spectroscopy.
33853047	4	18	theme	DOX	787:789	arg1	loading					768:774	the loading	764:774	the loading of CMC and DOX	764:789	The structure and crystalization of the UiO-66 MOFs were determined by the Transmitting Electron Microscope (TEM) and x-ray diffraction methods, while the loading of CMC and DOX was inspected by Fourier Transform InfraRed (FT-IR) and UV-vis spectroscopy.
33853047	4	19	theme	Electron	701:708	arg1	Microscope					710:719	the Transmitting Electron Microscope	684:719	the Transmitting Electron Microscope (TEM)	684:725	The structure and crystalization of the UiO-66 MOFs were determined by the Transmitting Electron Microscope (TEM) and x-ray diffraction methods, while the loading of CMC and DOX was inspected by Fourier Transform InfraRed (FT-IR) and UV-vis spectroscopy.
33853047	4	19	theme	Electron	701:708	arg1	TEM					722:724	TEM	722:724	TEM	722:724	The structure and crystalization of the UiO-66 MOFs were determined by the Transmitting Electron Microscope (TEM) and x-ray diffraction methods, while the loading of CMC and DOX was inspected by Fourier Transform InfraRed (FT-IR) and UV-vis spectroscopy.
33853047	3	20	theme	DOX	520:522	arg1	DOX					520:522	UiO-66/CMC@DOX	509:522	UiO-66/CMC@DOX	509:522	The composite of UiO-66/CMC@DOX was synthesized, and the loading capacity was as high as 45μg DOX per mg of the carrier.
33853047	3	20	theme	DOX	520:522	arg1	composite					496:504	The composite	492:504	The composite of UiO-66/CMC@DOX	492:522	The composite of UiO-66/CMC@DOX was synthesized, and the loading capacity was as high as 45μg DOX per mg of the carrier.
33853047	1	21	theme	mesoporous	170:179	arg1	frameworks					195:204	mesoporous metal-organic frameworks	170:204	mesoporous metal-organic frameworks (MOFs) of UiO-66 particles for pH-responsive doxorubicin (DOX) delivery and cancer treatment	170:297	This paper applied mesoporous metal-organic frameworks (MOFs) of UiO-66 particles for pH-responsive doxorubicin (DOX) delivery and cancer treatment.
33853047	1	21	theme	mesoporous	170:179	arg1	MOFs					207:210	MOFs	207:210	MOFs	207:210	This paper applied mesoporous metal-organic frameworks (MOFs) of UiO-66 particles for pH-responsive doxorubicin (DOX) delivery and cancer treatment.
33853047	0	22	theme	drug	110:113	arg1	delivery					115:122	efficient pH-responsive drug delivery	86:122	efficient pH-responsive drug delivery	86:122	Synthesis and surface modification of mesoporous metal-organic framework (UiO-66) for efficient pH-responsive drug delivery and lung cancer treatment.
33853047	4	23	dep	InfraRed	826:833	arg1	FT-IR					836:840	FT-IR	836:840	FT-IR	836:840	The structure and crystalization of the UiO-66 MOFs were determined by the Transmitting Electron Microscope (TEM) and x-ray diffraction methods, while the loading of CMC and DOX was inspected by Fourier Transform InfraRed (FT-IR) and UV-vis spectroscopy.
33853047	5	24	theme	DOX	872:874	arg1	release					876:882	The DOX release	868:882	The DOX release from UiO-66/CMC@DOX	868:902	The DOX release from UiO-66/CMC@DOX was tested under different pH at 37 °C.
33853047	1	25	theme	metal-organic	181:193	arg1	frameworks					195:204	mesoporous metal-organic frameworks	170:204	mesoporous metal-organic frameworks (MOFs) of UiO-66 particles for pH-responsive doxorubicin (DOX) delivery and cancer treatment	170:297	This paper applied mesoporous metal-organic frameworks (MOFs) of UiO-66 particles for pH-responsive doxorubicin (DOX) delivery and cancer treatment.
33853047	1	25	theme	metal-organic	181:193	arg1	MOFs					207:210	MOFs	207:210	MOFs	207:210	This paper applied mesoporous metal-organic frameworks (MOFs) of UiO-66 particles for pH-responsive doxorubicin (DOX) delivery and cancer treatment.
33853047	2	26	theme	chemotherapeutic	462:477	arg1	drug					479:482	the chemotherapeutic drug	458:482	the chemotherapeutic drug of DOX	458:489	Mesoporous structured UiO-66 MOFs were synthesized, and carboxymethylcellulose (CMC) was loaded for sensitive pH response and also as a linker to encapsulate the chemotherapeutic drug of DOX.
33853047	7	27	theme	DOX	1090:1092	arg1	solubility					1094:1103	higher DOX solubility	1083:1103	higher DOX solubility	1083:1103	A lower pH was more favorable for DOX release due to the CMC shrinking and higher DOX solubility in an acidic environment.
33853047	3	28	theme	loading	549:555	arg1	high					573:576	high	573:576	high	573:576	The composite of UiO-66/CMC@DOX was synthesized, and the loading capacity was as high as 45μg DOX per mg of the carrier.
33853047	3	28	theme	loading	549:555	arg1	capacity					557:564	the loading capacity	545:564	the loading capacity	545:564	The composite of UiO-66/CMC@DOX was synthesized, and the loading capacity was as high as 45μg DOX per mg of the carrier.
33853047	2	29	theme	UiO-66	322:327	arg1	MOFs					329:332	Mesoporous structured UiO-66 MOFs	300:332	Mesoporous structured UiO-66 MOFs	300:332	Mesoporous structured UiO-66 MOFs were synthesized, and carboxymethylcellulose (CMC) was loaded for sensitive pH response and also as a linker to encapsulate the chemotherapeutic drug of DOX.
33853047	8	30	theme	A549	1215:1218	arg1	%					1282:1282	28%	1280:1282	28%	1280:1282	The cytotoxicity study indicated that, under the DOX concentration of 4μg ml-1, the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC was 28%, which was lower than that from free DOX solution (47%).
33853047	8	30	theme	A549	1215:1218	arg1	viability					1252:1260	the A549 cell (Lung Carcinoma Cell Line) viability	1211:1260	the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC	1211:1274	The cytotoxicity study indicated that, under the DOX concentration of 4μg ml-1, the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC was 28%, which was lower than that from free DOX solution (47%).
33853047	8	30	theme	A549	1215:1218	arg1	Line					1246:1249	Lung Carcinoma Cell Line	1226:1249	Lung Carcinoma Cell Line	1226:1249	The cytotoxicity study indicated that, under the DOX concentration of 4μg ml-1, the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC was 28%, which was lower than that from free DOX solution (47%).
33853047	7	31	theme	DOX	1042:1044	arg1	release					1046:1052	DOX release	1042:1052	DOX release	1042:1052	A lower pH was more favorable for DOX release due to the CMC shrinking and higher DOX solubility in an acidic environment.
33853047	1	32	theme	cancer	282:287	arg1	treatment					289:297	cancer treatment	282:297	cancer treatment	282:297	This paper applied mesoporous metal-organic frameworks (MOFs) of UiO-66 particles for pH-responsive doxorubicin (DOX) delivery and cancer treatment.
33853047	0	33	theme	lung	128:131	arg1	cancer					133:138	lung cancer	128:138	lung cancer treatment	128:148	Synthesis and surface modification of mesoporous metal-organic framework (UiO-66) for efficient pH-responsive drug delivery and lung cancer treatment.
33853047	2	34	theme	structured	311:320	arg1	MOFs					329:332	Mesoporous structured UiO-66 MOFs	300:332	Mesoporous structured UiO-66 MOFs	300:332	Mesoporous structured UiO-66 MOFs were synthesized, and carboxymethylcellulose (CMC) was loaded for sensitive pH response and also as a linker to encapsulate the chemotherapeutic drug of DOX.
33853047	8	35	theme	cytotoxicity	1135:1146	arg1	study					1148:1152	The cytotoxicity study	1131:1152	The cytotoxicity study	1131:1152	The cytotoxicity study indicated that, under the DOX concentration of 4μg ml-1, the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC was 28%, which was lower than that from free DOX solution (47%).
33853047	0	36	theme	surface	14:20	arg1	modification					22:33	surface modification	14:33	surface modification	14:33	Synthesis and surface modification of mesoporous metal-organic framework (UiO-66) for efficient pH-responsive drug delivery and lung cancer treatment.
33853047	4	37	theme	x-ray	731:735	arg1	methods					749:755	x-ray diffraction methods	731:755	x-ray diffraction methods	731:755	The structure and crystalization of the UiO-66 MOFs were determined by the Transmitting Electron Microscope (TEM) and x-ray diffraction methods, while the loading of CMC and DOX was inspected by Fourier Transform InfraRed (FT-IR) and UV-vis spectroscopy.
33853047	3	38	theme	carrier	604:610	arg1	mg					594:595	mg	594:595	mg of the carrier	594:610	The composite of UiO-66/CMC@DOX was synthesized, and the loading capacity was as high as 45μg DOX per mg of the carrier.
33853047	2	39	theme	Mesoporous	300:309	arg1	MOFs					329:332	Mesoporous structured UiO-66 MOFs	300:332	Mesoporous structured UiO-66 MOFs	300:332	Mesoporous structured UiO-66 MOFs were synthesized, and carboxymethylcellulose (CMC) was loaded for sensitive pH response and also as a linker to encapsulate the chemotherapeutic drug of DOX.
33853047	6	40	theme	5	1005:1005	arg1	pH					999:1000	the pH	995:1000	the pH of 5	995:1005	The DOX accumulative release could reach 78% under the pH of 5.
33853047	0	41	theme	mesoporous	38:47	arg1	UiO-66					74:79	UiO-66	74:79	UiO-66	74:79	Synthesis and surface modification of mesoporous metal-organic framework (UiO-66) for efficient pH-responsive drug delivery and lung cancer treatment.
33853047	0	41	theme	mesoporous	38:47	arg1	framework					63:71	mesoporous metal-organic framework	38:71	mesoporous metal-organic framework (UiO-66)	38:80	Synthesis and surface modification of mesoporous metal-organic framework (UiO-66) for efficient pH-responsive drug delivery and lung cancer treatment.
33853047	5	42	from	DOX	900:902	arg1	release					876:882	The DOX release	868:882	The DOX release from UiO-66/CMC@DOX	868:902	The DOX release from UiO-66/CMC@DOX was tested under different pH at 37 °C.
33853047	9	43	theme	drug	1390:1393	arg1	carrier					1404:1410	an efficient drug delivery carrier	1377:1410	an efficient drug delivery carrier for chemotherapeutic drug and release	1377:1448	UiO-66 MOFs were demonstrated to be an efficient drug delivery carrier for chemotherapeutic drug and release.
33853047	9	43	theme	drug	1390:1393	arg1	MOFs					1348:1351	UiO-66 MOFs	1341:1351	UiO-66 MOFs	1341:1351	UiO-66 MOFs were demonstrated to be an efficient drug delivery carrier for chemotherapeutic drug and release.
33853047	6	44	theme	accumulative	952:963	arg1	release					965:971	The DOX accumulative release	944:971	The DOX accumulative release	944:971	The DOX accumulative release could reach 78% under the pH of 5.
33853047	2	45	theme	sensitive	400:408	arg1	response					413:420	sensitive pH response	400:420	sensitive pH response	400:420	Mesoporous structured UiO-66 MOFs were synthesized, and carboxymethylcellulose (CMC) was loaded for sensitive pH response and also as a linker to encapsulate the chemotherapeutic drug of DOX.
33853047	0	46	theme	cancer	133:138	arg1	treatment					140:148	lung cancer treatment	128:148	lung cancer treatment	128:148	Synthesis and surface modification of mesoporous metal-organic framework (UiO-66) for efficient pH-responsive drug delivery and lung cancer treatment.
33853047	7	47	from	shrinking	1069:1077	arg1	environment					1118:1128	an acidic environment	1108:1128	an acidic environment	1108:1128	A lower pH was more favorable for DOX release due to the CMC shrinking and higher DOX solubility in an acidic environment.
33853047	8	48	theme	Cell	1241:1244	arg1	viability					1252:1260	the A549 cell (Lung Carcinoma Cell Line) viability	1211:1260	the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC	1211:1274	The cytotoxicity study indicated that, under the DOX concentration of 4μg ml-1, the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC was 28%, which was lower than that from free DOX solution (47%).
33853047	8	48	theme	Cell	1241:1244	arg1	Line					1246:1249	Lung Carcinoma Cell Line	1226:1249	Lung Carcinoma Cell Line	1226:1249	The cytotoxicity study indicated that, under the DOX concentration of 4μg ml-1, the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC was 28%, which was lower than that from free DOX solution (47%).
33853047	1	49	theme	UiO-66	216:221	arg1	particles					223:231	UiO-66 particles	216:231	UiO-66 particles	216:231	This paper applied mesoporous metal-organic frameworks (MOFs) of UiO-66 particles for pH-responsive doxorubicin (DOX) delivery and cancer treatment.
33853047	0	50	theme	framework	63:71	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis	0:8	Synthesis and surface modification of mesoporous metal-organic framework (UiO-66) for efficient pH-responsive drug delivery and lung cancer treatment.
33853047	0	50	theme	framework	63:71	arg1	modification					22:33	surface modification	14:33	surface modification	14:33	Synthesis and surface modification of mesoporous metal-organic framework (UiO-66) for efficient pH-responsive drug delivery and lung cancer treatment.
33853047	0	51	mod	modification	22:33	arg3	surface					14:20	surface modification	14:33	surface modification	14:33	Synthesis and surface modification of mesoporous metal-organic framework (UiO-66) for efficient pH-responsive drug delivery and lung cancer treatment.
33853047	0	51	mod	modification	22:33	arg1	UiO-66					74:79	UiO-66	74:79	UiO-66	74:79	Synthesis and surface modification of mesoporous metal-organic framework (UiO-66) for efficient pH-responsive drug delivery and lung cancer treatment.
33853047	0	51	mod	modification	22:33	arg1	framework					63:71	mesoporous metal-organic framework	38:71	mesoporous metal-organic framework (UiO-66)	38:80	Synthesis and surface modification of mesoporous metal-organic framework (UiO-66) for efficient pH-responsive drug delivery and lung cancer treatment.
33853047	6	52	theme	DOX	948:950	arg1	release					965:971	The DOX accumulative release	944:971	The DOX accumulative release	944:971	The DOX accumulative release could reach 78% under the pH of 5.
33853047	4	53	theme	CMC	779:781	arg1	loading					768:774	the loading	764:774	the loading of CMC and DOX	764:789	The structure and crystalization of the UiO-66 MOFs were determined by the Transmitting Electron Microscope (TEM) and x-ray diffraction methods, while the loading of CMC and DOX was inspected by Fourier Transform InfraRed (FT-IR) and UV-vis spectroscopy.
33853047	8	54	theme	cell	1220:1223	arg1	%					1282:1282	28%	1280:1282	28%	1280:1282	The cytotoxicity study indicated that, under the DOX concentration of 4μg ml-1, the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC was 28%, which was lower than that from free DOX solution (47%).
33853047	8	54	theme	cell	1220:1223	arg1	viability					1252:1260	the A549 cell (Lung Carcinoma Cell Line) viability	1211:1260	the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC	1211:1274	The cytotoxicity study indicated that, under the DOX concentration of 4μg ml-1, the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC was 28%, which was lower than that from free DOX solution (47%).
33853047	8	54	theme	cell	1220:1223	arg1	Line					1246:1249	Lung Carcinoma Cell Line	1226:1249	Lung Carcinoma Cell Line	1226:1249	The cytotoxicity study indicated that, under the DOX concentration of 4μg ml-1, the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC was 28%, which was lower than that from free DOX solution (47%).
33853047	0	55	theme	metal-organic	49:61	arg1	UiO-66					74:79	UiO-66	74:79	UiO-66	74:79	Synthesis and surface modification of mesoporous metal-organic framework (UiO-66) for efficient pH-responsive drug delivery and lung cancer treatment.
33853047	0	55	theme	metal-organic	49:61	arg1	framework					63:71	mesoporous metal-organic framework	38:71	mesoporous metal-organic framework (UiO-66)	38:80	Synthesis and surface modification of mesoporous metal-organic framework (UiO-66) for efficient pH-responsive drug delivery and lung cancer treatment.
33853047	7	56	theme	CMC	1065:1067	arg1	shrinking					1069:1077	CMC shrinking	1065:1077	CMC shrinking	1065:1077	A lower pH was more favorable for DOX release due to the CMC shrinking and higher DOX solubility in an acidic environment.
33853047	7	57	dep	shrinking	1069:1077	arg1	the					1061:1063	the	1061:1063	the	1061:1063	A lower pH was more favorable for DOX release due to the CMC shrinking and higher DOX solubility in an acidic environment.
33853047	9	58	theme	chemotherapeutic	1416:1431	arg1	drug					1433:1436	chemotherapeutic drug	1416:1436	chemotherapeutic drug	1416:1436	UiO-66 MOFs were demonstrated to be an efficient drug delivery carrier for chemotherapeutic drug and release.
33853047	8	59	theme	UiO-66/CMC	1265:1274	arg1	%					1282:1282	28%	1280:1282	28%	1280:1282	The cytotoxicity study indicated that, under the DOX concentration of 4μg ml-1, the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC was 28%, which was lower than that from free DOX solution (47%).
33853047	8	59	theme	UiO-66/CMC	1265:1274	arg1	viability					1252:1260	the A549 cell (Lung Carcinoma Cell Line) viability	1211:1260	the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC	1211:1274	The cytotoxicity study indicated that, under the DOX concentration of 4μg ml-1, the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC was 28%, which was lower than that from free DOX solution (47%).
33853047	8	59	theme	UiO-66/CMC	1265:1274	arg1	Line					1246:1249	Lung Carcinoma Cell Line	1226:1249	Lung Carcinoma Cell Line	1226:1249	The cytotoxicity study indicated that, under the DOX concentration of 4μg ml-1, the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC was 28%, which was lower than that from free DOX solution (47%).
33853047	8	60	theme	Lung	1226:1229	arg1	viability					1252:1260	the A549 cell (Lung Carcinoma Cell Line) viability	1211:1260	the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC	1211:1274	The cytotoxicity study indicated that, under the DOX concentration of 4μg ml-1, the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC was 28%, which was lower than that from free DOX solution (47%).
33853047	8	60	theme	Lung	1226:1229	arg1	Line					1246:1249	Lung Carcinoma Cell Line	1226:1249	Lung Carcinoma Cell Line	1226:1249	The cytotoxicity study indicated that, under the DOX concentration of 4μg ml-1, the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC was 28%, which was lower than that from free DOX solution (47%).
33853047	8	61	theme	Carcinoma	1231:1239	arg1	viability					1252:1260	the A549 cell (Lung Carcinoma Cell Line) viability	1211:1260	the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC	1211:1274	The cytotoxicity study indicated that, under the DOX concentration of 4μg ml-1, the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC was 28%, which was lower than that from free DOX solution (47%).
33853047	8	61	theme	Carcinoma	1231:1239	arg1	Line					1246:1249	Lung Carcinoma Cell Line	1226:1249	Lung Carcinoma Cell Line	1226:1249	The cytotoxicity study indicated that, under the DOX concentration of 4μg ml-1, the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC was 28%, which was lower than that from free DOX solution (47%).
33853047	1	62	theme	particles	223:231	arg1	frameworks					195:204	mesoporous metal-organic frameworks	170:204	mesoporous metal-organic frameworks (MOFs) of UiO-66 particles for pH-responsive doxorubicin (DOX) delivery and cancer treatment	170:297	This paper applied mesoporous metal-organic frameworks (MOFs) of UiO-66 particles for pH-responsive doxorubicin (DOX) delivery and cancer treatment.
33853047	1	62	theme	particles	223:231	arg1	MOFs					207:210	MOFs	207:210	MOFs	207:210	This paper applied mesoporous metal-organic frameworks (MOFs) of UiO-66 particles for pH-responsive doxorubicin (DOX) delivery and cancer treatment.
33853047	7	63	from	solubility	1094:1103	arg1	environment					1118:1128	an acidic environment	1108:1128	an acidic environment	1108:1128	A lower pH was more favorable for DOX release due to the CMC shrinking and higher DOX solubility in an acidic environment.
33853047	4	64	theme	diffraction	737:747	arg1	methods					749:755	x-ray diffraction methods	731:755	x-ray diffraction methods	731:755	The structure and crystalization of the UiO-66 MOFs were determined by the Transmitting Electron Microscope (TEM) and x-ray diffraction methods, while the loading of CMC and DOX was inspected by Fourier Transform InfraRed (FT-IR) and UV-vis spectroscopy.
33853047	8	65	theme	free	1316:1319	arg1	solution					1325:1332	free DOX solution	1316:1332	free DOX solution (47%)	1316:1338	The cytotoxicity study indicated that, under the DOX concentration of 4μg ml-1, the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC was 28%, which was lower than that from free DOX solution (47%).
33853047	8	65	theme	free	1316:1319	arg1	%					1337:1337	47%	1335:1337	47%	1335:1337	The cytotoxicity study indicated that, under the DOX concentration of 4μg ml-1, the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC was 28%, which was lower than that from free DOX solution (47%).
33853047	8	66	theme	DOX	1180:1182	arg1	concentration					1184:1196	the DOX concentration	1176:1196	the DOX concentration of 4μg ml-1	1176:1208	The cytotoxicity study indicated that, under the DOX concentration of 4μg ml-1, the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC was 28%, which was lower than that from free DOX solution (47%).
33853047	2	67	theme	pH	410:411	arg1	response					413:420	sensitive pH response	400:420	sensitive pH response	400:420	Mesoporous structured UiO-66 MOFs were synthesized, and carboxymethylcellulose (CMC) was loaded for sensitive pH response and also as a linker to encapsulate the chemotherapeutic drug of DOX.
33853047	3	68	theme	45μg	581:584	arg1	DOX					586:588	45μg DOX	581:588	45μg DOX	581:588	The composite of UiO-66/CMC@DOX was synthesized, and the loading capacity was as high as 45μg DOX per mg of the carrier.
33853047	3	69	theme	UiO-66/CMC	509:518	arg1	DOX					520:522	UiO-66/CMC@DOX	509:522	UiO-66/CMC@DOX	509:522	The composite of UiO-66/CMC@DOX was synthesized, and the loading capacity was as high as 45μg DOX per mg of the carrier.
33853047	1	70	theme	pH-responsive	237:249	arg1	delivery					269:276	pH-responsive doxorubicin (DOX) delivery	237:276	pH-responsive doxorubicin (DOX) delivery	237:276	This paper applied mesoporous metal-organic frameworks (MOFs) of UiO-66 particles for pH-responsive doxorubicin (DOX) delivery and cancer treatment.
33853047	4	71	dep	Fourier	808:814	arg1	Transform					816:824	Transform	816:824	Transform InfraRed (FT-IR) and UV-vis spectroscopy	816:865	The structure and crystalization of the UiO-66 MOFs were determined by the Transmitting Electron Microscope (TEM) and x-ray diffraction methods, while the loading of CMC and DOX was inspected by Fourier Transform InfraRed (FT-IR) and UV-vis spectroscopy.
33853047	8	72	theme	DOX	1321:1323	arg1	solution					1325:1332	free DOX solution	1316:1332	free DOX solution (47%)	1316:1338	The cytotoxicity study indicated that, under the DOX concentration of 4μg ml-1, the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC was 28%, which was lower than that from free DOX solution (47%).
33853047	8	72	theme	DOX	1321:1323	arg1	%					1337:1337	47%	1335:1337	47%	1335:1337	The cytotoxicity study indicated that, under the DOX concentration of 4μg ml-1, the A549 cell (Lung Carcinoma Cell Line) viability of UiO-66/CMC was 28%, which was lower than that from free DOX solution (47%).
33853047	3	73	theme	@	519:519	arg1	DOX					520:522	UiO-66/CMC@DOX	509:522	UiO-66/CMC@DOX	509:522	The composite of UiO-66/CMC@DOX was synthesized, and the loading capacity was as high as 45μg DOX per mg of the carrier.
33853047	1	74	theme	doxorubicin	251:261	arg1	delivery					269:276	pH-responsive doxorubicin (DOX) delivery	237:276	pH-responsive doxorubicin (DOX) delivery	237:276	This paper applied mesoporous metal-organic frameworks (MOFs) of UiO-66 particles for pH-responsive doxorubicin (DOX) delivery and cancer treatment.
34382257	10	0	theme	}	1597:1597	arg1	systems					1605:1611	novel {Mo154 }-based systems	1584:1611	novel {Mo154 }-based systems	1584:1611	Overall, this work paves the way for the development of novel {Mo154 }-based systems, incorporated in self-healing and injectable hydrogels for dual chemo-photothermal therapy.
34382257	6	1	theme	room	964:967	arg1	temperature					969:979	room temperature	964:979	room temperature	964:979	The gelation occurs within 60 s at room temperature, and the dual crosslinking by Schiff base and electrostatic interactions generates a dynamic network, which enables self-healing after injection.
34382257	1	2	theme	molecular	162:170	arg1	clusters					172:179	molecular clusters	162:179	molecular clusters	162:179	Polyoxometalates are an emerging class of molecular clusters, with well-defined structures and chemical compositions that are produced through simple, low-cost, and highly reproducible methods.
34382257	4	3	theme	injectable	590:599	arg1	hydrogel					618:625	an injectable and self-healing hydrogel	587:625	an injectable and self-healing hydrogel of easy preparation and administration	587:664	Herein, an injectable and self-healing hydrogel of easy preparation and administration is developed, incorporating both {Mo154 } and doxorubicin for synergistic photothermal and chemotherapy applications.
34382257	0	4	theme	NIR	69:71	arg1	Responsiveness					73:86	NIR Responsiveness	69:86	NIR Responsiveness	69:86	Dual-Crosslinked Dynamic Hydrogel Incorporating {Mo154 } with pH and NIR Responsiveness for Chemo-Photothermal Therapy.
34382257	1	5	theme	clusters	172:179	arg1	Polyoxometalates					120:135	Polyoxometalates	120:135	Polyoxometalates	120:135	Polyoxometalates are an emerging class of molecular clusters, with well-defined structures and chemical compositions that are produced through simple, low-cost, and highly reproducible methods.
34382257	1	5	theme	clusters	172:179	arg1	class					153:157	an emerging class	141:157	an emerging class of molecular clusters	141:179	Polyoxometalates are an emerging class of molecular clusters, with well-defined structures and chemical compositions that are produced through simple, low-cost, and highly reproducible methods.
34382257	1	6	theme	low-cost	271:278	arg1	methods					305:311	simple, low-cost, and highly reproducible methods	263:311	simple, low-cost, and highly reproducible methods	263:311	Polyoxometalates are an emerging class of molecular clusters, with well-defined structures and chemical compositions that are produced through simple, low-cost, and highly reproducible methods.
34382257	0	7	theme	Chemo-Photothermal	92:109	arg1	Therapy					111:117	Chemo-Photothermal Therapy	92:117	Chemo-Photothermal Therapy	92:117	Dual-Crosslinked Dynamic Hydrogel Incorporating {Mo154 } with pH and NIR Responsiveness for Chemo-Photothermal Therapy.
34382257	4	8	theme	easy	630:633	arg1	preparation					635:645	easy preparation	630:645	easy preparation	630:645	Herein, an injectable and self-healing hydrogel of easy preparation and administration is developed, incorporating both {Mo154 } and doxorubicin for synergistic photothermal and chemotherapy applications.
34382257	1	9	with	class	153:157	arg1	structures					200:209	well-defined structures	187:209	well-defined structures	187:209	Polyoxometalates are an emerging class of molecular clusters, with well-defined structures and chemical compositions that are produced through simple, low-cost, and highly reproducible methods.
34382257	1	9	with	class	153:157	arg1	compositions					224:235	chemical compositions	215:235	chemical compositions	215:235	Polyoxometalates are an emerging class of molecular clusters, with well-defined structures and chemical compositions that are produced through simple, low-cost, and highly reproducible methods.
34382257	3	10	theme	system	555:560	arg1	development					519:529	the development	515:529	the development of a localized delivery system still incipient	515:576	However, its toxicity hinders its systemic administration, being the development of a localized delivery system still incipient.
34382257	4	11	theme	self-healing	605:616	arg1	hydrogel					618:625	an injectable and self-healing hydrogel	587:625	an injectable and self-healing hydrogel of easy preparation and administration	587:664	Herein, an injectable and self-healing hydrogel of easy preparation and administration is developed, incorporating both {Mo154 } and doxorubicin for synergistic photothermal and chemotherapy applications.
34382257	7	12	theme	pH	1251:1252	arg1	changes					1254:1260	pH changes	1251:1260	pH changes	1251:1260	Moreover, the hydrogel delivers chemotherapeutic drugs, with a release triggered by dual near infra-red (NIR) radiation and pH changes.
34382257	10	13	theme	chemo-photothermal	1677:1694	arg1	therapy					1696:1702	dual chemo-photothermal therapy	1672:1702	dual chemo-photothermal therapy	1672:1702	Overall, this work paves the way for the development of novel {Mo154 }-based systems, incorporated in self-healing and injectable hydrogels for dual chemo-photothermal therapy.
34382257	8	14	theme	photothermal	1404:1415	arg1	combination					1389:1399	the simultaneous combination	1372:1399	the simultaneous combination of photothermal and chemotherapy	1372:1432	This stimuli-responsive release system along with the photothermal conversion ability of the hydrogel allows the simultaneous combination of photothermal and chemotherapy.
34382257	2	15	theme	wheel-shaped	333:344	arg1	agent					391:395	a promising photothermal agent	366:395	a promising photothermal agent due to its intervalence charge transfer transitions	366:447	In particular, the wheel-shaped cluster {Mo154 } is a promising photothermal agent due to its intervalence charge transfer transitions.
34382257	2	15	theme	wheel-shaped	333:344	arg1	}					361:361	the wheel-shaped cluster {Mo154 }	329:361	the wheel-shaped cluster {Mo154 }	329:361	In particular, the wheel-shaped cluster {Mo154 } is a promising photothermal agent due to its intervalence charge transfer transitions.
34382257	4	16	theme	synergistic	728:738	arg1	photothermal					740:751	synergistic photothermal	728:751	synergistic photothermal	728:751	Herein, an injectable and self-healing hydrogel of easy preparation and administration is developed, incorporating both {Mo154 } and doxorubicin for synergistic photothermal and chemotherapy applications.
34382257	5	17	theme	functionalized	891:904	arg1	chitosan					906:913	poly(N-isopropylacrylamide) functionalized chitosan	863:913	poly(N-isopropylacrylamide) functionalized chitosan	863:913	The hydrogel is composed of benzylaldehyde functionalized polyethylene glycol, poly(N-isopropylacrylamide) functionalized chitosan and {Mo154 }.
34382257	2	18	theme	charge	421:426	arg1	transitions					437:447	its intervalence charge transfer transitions	404:447	its intervalence charge transfer transitions	404:447	In particular, the wheel-shaped cluster {Mo154 } is a promising photothermal agent due to its intervalence charge transfer transitions.
34382257	3	19	theme	localized	536:544	arg1	system					555:560	a localized delivery system	534:560	a localized delivery system still incipient	534:576	However, its toxicity hinders its systemic administration, being the development of a localized delivery system still incipient.
34382257	4	20	theme	{	699:699	arg1	}					706:706	{Mo154 }	699:706	{Mo154 }	699:706	Herein, an injectable and self-healing hydrogel of easy preparation and administration is developed, incorporating both {Mo154 } and doxorubicin for synergistic photothermal and chemotherapy applications.
34382257	5	21	theme	{	919:919	arg1	}					926:926	{Mo154 }	919:926	{Mo154 }	919:926	The hydrogel is composed of benzylaldehyde functionalized polyethylene glycol, poly(N-isopropylacrylamide) functionalized chitosan and {Mo154 }.
34382257	5	22	theme	benzylaldehyde	812:825	arg1	glycol					855:860	benzylaldehyde functionalized polyethylene glycol	812:860	benzylaldehyde functionalized polyethylene glycol	812:860	The hydrogel is composed of benzylaldehyde functionalized polyethylene glycol, poly(N-isopropylacrylamide) functionalized chitosan and {Mo154 }.
34382257	3	23	theme	delivery	546:553	arg1	system					555:560	a localized delivery system	534:560	a localized delivery system still incipient	534:576	However, its toxicity hinders its systemic administration, being the development of a localized delivery system still incipient.
34382257	2	24	theme	due	397:399	arg1	agent					391:395	a promising photothermal agent	366:395	a promising photothermal agent due to its intervalence charge transfer transitions	366:447	In particular, the wheel-shaped cluster {Mo154 } is a promising photothermal agent due to its intervalence charge transfer transitions.
34382257	2	24	theme	due	397:399	arg1	}					361:361	the wheel-shaped cluster {Mo154 }	329:361	the wheel-shaped cluster {Mo154 }	329:361	In particular, the wheel-shaped cluster {Mo154 } is a promising photothermal agent due to its intervalence charge transfer transitions.
34382257	0	25	theme	Dynamic	17:23	arg1	Hydrogel					25:32	Dual-Crosslinked Dynamic Hydrogel	0:32	Dual-Crosslinked Dynamic Hydrogel	0:32	Dual-Crosslinked Dynamic Hydrogel Incorporating {Mo154 } with pH and NIR Responsiveness for Chemo-Photothermal Therapy.
34382257	10	26	theme	Mo154	1591:1595	arg1	}					1597:1597	novel {Mo154 }	1584:1597	novel {Mo154 }-based systems	1584:1611	Overall, this work paves the way for the development of novel {Mo154 }-based systems, incorporated in self-healing and injectable hydrogels for dual chemo-photothermal therapy.
34382257	10	27	theme	self-healing	1630:1641	arg1	hydrogels					1658:1666	self-healing and injectable hydrogels	1630:1666	self-healing and injectable hydrogels for dual chemo-photothermal therapy	1630:1702	Overall, this work paves the way for the development of novel {Mo154 }-based systems, incorporated in self-healing and injectable hydrogels for dual chemo-photothermal therapy.
34382257	10	28	theme	injectable	1647:1656	arg1	hydrogels					1658:1666	self-healing and injectable hydrogels	1630:1666	self-healing and injectable hydrogels for dual chemo-photothermal therapy	1630:1702	Overall, this work paves the way for the development of novel {Mo154 }-based systems, incorporated in self-healing and injectable hydrogels for dual chemo-photothermal therapy.
34382257	1	29	theme	well-defined	187:198	arg1	structures					200:209	well-defined structures	187:209	well-defined structures	187:209	Polyoxometalates are an emerging class of molecular clusters, with well-defined structures and chemical compositions that are produced through simple, low-cost, and highly reproducible methods.
34382257	0	30	theme	Dual-Crosslinked	0:15	arg1	Hydrogel					25:32	Dual-Crosslinked Dynamic Hydrogel	0:32	Dual-Crosslinked Dynamic Hydrogel	0:32	Dual-Crosslinked Dynamic Hydrogel Incorporating {Mo154 } with pH and NIR Responsiveness for Chemo-Photothermal Therapy.
34382257	7	31	theme	dual	1211:1214	arg1	radiation					1237:1245	dual near infra-red (NIR) radiation	1211:1245	dual near infra-red (NIR) radiation	1211:1245	Moreover, the hydrogel delivers chemotherapeutic drugs, with a release triggered by dual near infra-red (NIR) radiation and pH changes.
34382257	7	31	theme	dual	1211:1214	arg1	NIR					1232:1234	NIR	1232:1234	NIR	1232:1234	Moreover, the hydrogel delivers chemotherapeutic drugs, with a release triggered by dual near infra-red (NIR) radiation and pH changes.
34382257	4	32	dep	photothermal	740:751	arg1	applications					770:781	applications	770:781	applications	770:781	Herein, an injectable and self-healing hydrogel of easy preparation and administration is developed, incorporating both {Mo154 } and doxorubicin for synergistic photothermal and chemotherapy applications.
34382257	2	33	theme	photothermal	378:389	arg1	agent					391:395	a promising photothermal agent	366:395	a promising photothermal agent due to its intervalence charge transfer transitions	366:447	In particular, the wheel-shaped cluster {Mo154 } is a promising photothermal agent due to its intervalence charge transfer transitions.
34382257	2	33	theme	photothermal	378:389	arg1	}					361:361	the wheel-shaped cluster {Mo154 }	329:361	the wheel-shaped cluster {Mo154 }	329:361	In particular, the wheel-shaped cluster {Mo154 } is a promising photothermal agent due to its intervalence charge transfer transitions.
34382257	7	34	theme	near	1216:1219	arg1	radiation					1237:1245	dual near infra-red (NIR) radiation	1211:1245	dual near infra-red (NIR) radiation	1211:1245	Moreover, the hydrogel delivers chemotherapeutic drugs, with a release triggered by dual near infra-red (NIR) radiation and pH changes.
34382257	7	34	theme	near	1216:1219	arg1	NIR					1232:1234	NIR	1232:1234	NIR	1232:1234	Moreover, the hydrogel delivers chemotherapeutic drugs, with a release triggered by dual near infra-red (NIR) radiation and pH changes.
34382257	1	35	theme	reproducible	292:303	arg1	methods					305:311	simple, low-cost, and highly reproducible methods	263:311	simple, low-cost, and highly reproducible methods	263:311	Polyoxometalates are an emerging class of molecular clusters, with well-defined structures and chemical compositions that are produced through simple, low-cost, and highly reproducible methods.
34382257	8	36	theme	hydrogel	1356:1363	arg1	ability					1341:1347	the photothermal conversion ability	1313:1347	This stimuli-responsive release system along with the photothermal conversion ability of the hydrogel	1263:1363	This stimuli-responsive release system along with the photothermal conversion ability of the hydrogel allows the simultaneous combination of photothermal and chemotherapy.
34382257	0	37	link	Dual-Crosslinked	0:15	arg1	Hydrogel					25:32	Dual-Crosslinked Dynamic Hydrogel	0:32	Dual-Crosslinked Dynamic Hydrogel	0:32	Dual-Crosslinked Dynamic Hydrogel Incorporating {Mo154 } with pH and NIR Responsiveness for Chemo-Photothermal Therapy.
34382257	3	38	theme	incipient	568:576	arg1	system					555:560	a localized delivery system	534:560	a localized delivery system still incipient	534:576	However, its toxicity hinders its systemic administration, being the development of a localized delivery system still incipient.
34382257	2	39	theme	transfer	428:435	arg1	transitions					437:447	its intervalence charge transfer transitions	404:447	its intervalence charge transfer transitions	404:447	In particular, the wheel-shaped cluster {Mo154 } is a promising photothermal agent due to its intervalence charge transfer transitions.
34382257	7	40	theme	infra-red	1221:1229	arg1	radiation					1237:1245	dual near infra-red (NIR) radiation	1211:1245	dual near infra-red (NIR) radiation	1211:1245	Moreover, the hydrogel delivers chemotherapeutic drugs, with a release triggered by dual near infra-red (NIR) radiation and pH changes.
34382257	7	40	theme	infra-red	1221:1229	arg1	NIR					1232:1234	NIR	1232:1234	NIR	1232:1234	Moreover, the hydrogel delivers chemotherapeutic drugs, with a release triggered by dual near infra-red (NIR) radiation and pH changes.
34382257	8	41	theme	photothermal	1317:1328	arg1	ability					1341:1347	the photothermal conversion ability	1313:1347	This stimuli-responsive release system along with the photothermal conversion ability of the hydrogel	1263:1363	This stimuli-responsive release system along with the photothermal conversion ability of the hydrogel allows the simultaneous combination of photothermal and chemotherapy.
34382257	10	42	theme	-based	1598:1603	arg1	systems					1605:1611	novel {Mo154 }-based systems	1584:1611	novel {Mo154 }-based systems	1584:1611	Overall, this work paves the way for the development of novel {Mo154 }-based systems, incorporated in self-healing and injectable hydrogels for dual chemo-photothermal therapy.
34382257	1	43	theme	chemical	215:222	arg1	compositions					224:235	chemical compositions	215:235	chemical compositions	215:235	Polyoxometalates are an emerging class of molecular clusters, with well-defined structures and chemical compositions that are produced through simple, low-cost, and highly reproducible methods.
34382257	0	44	theme	Mo154	49:53	arg1	}					55:55	{Mo154 }	48:55	{Mo154 } with pH and NIR Responsiveness for Chemo-Photothermal Therapy	48:117	Dual-Crosslinked Dynamic Hydrogel Incorporating {Mo154 } with pH and NIR Responsiveness for Chemo-Photothermal Therapy.
34382257	2	45	theme	intervalence	408:419	arg1	transitions					437:447	its intervalence charge transfer transitions	404:447	its intervalence charge transfer transitions	404:447	In particular, the wheel-shaped cluster {Mo154 } is a promising photothermal agent due to its intervalence charge transfer transitions.
34382257	0	46	theme	{	48:48	arg1	}					55:55	{Mo154 }	48:55	{Mo154 } with pH and NIR Responsiveness for Chemo-Photothermal Therapy	48:117	Dual-Crosslinked Dynamic Hydrogel Incorporating {Mo154 } with pH and NIR Responsiveness for Chemo-Photothermal Therapy.
34382257	6	47	theme	dual	990:993	arg1	crosslinking					995:1006	the dual crosslinking	986:1006	the dual crosslinking by Schiff base and electrostatic interactions	986:1052	The gelation occurs within 60 s at room temperature, and the dual crosslinking by Schiff base and electrostatic interactions generates a dynamic network, which enables self-healing after injection.
34382257	5	48	theme	functionalized	827:840	arg1	glycol					855:860	benzylaldehyde functionalized polyethylene glycol	812:860	benzylaldehyde functionalized polyethylene glycol	812:860	The hydrogel is composed of benzylaldehyde functionalized polyethylene glycol, poly(N-isopropylacrylamide) functionalized chitosan and {Mo154 }.
34382257	6	49	theme	dynamic	1066:1072	arg1	network					1074:1080	a dynamic network	1064:1080	a dynamic network	1064:1080	The gelation occurs within 60 s at room temperature, and the dual crosslinking by Schiff base and electrostatic interactions generates a dynamic network, which enables self-healing after injection.
34382257	2	50	from	agent	391:395	arg1	particular					317:326	particular	317:326	particular	317:326	In particular, the wheel-shaped cluster {Mo154 } is a promising photothermal agent due to its intervalence charge transfer transitions.
34382257	8	51	theme	conversion	1330:1339	arg1	ability					1341:1347	the photothermal conversion ability	1313:1347	This stimuli-responsive release system along with the photothermal conversion ability of the hydrogel	1263:1363	This stimuli-responsive release system along with the photothermal conversion ability of the hydrogel allows the simultaneous combination of photothermal and chemotherapy.
34382257	5	52	theme	polyethylene	842:853	arg1	glycol					855:860	benzylaldehyde functionalized polyethylene glycol	812:860	benzylaldehyde functionalized polyethylene glycol	812:860	The hydrogel is composed of benzylaldehyde functionalized polyethylene glycol, poly(N-isopropylacrylamide) functionalized chitosan and {Mo154 }.
34382257	9	53	theme	synergic	1440:1447	arg1	system					1449:1454	This synergic system	1435:1454	This synergic system	1435:1454	This synergic system efficiently ablates the cancer tumor in vivo with no systemic toxicity.
34382257	10	54	theme	{	1590:1590	arg1	}					1597:1597	novel {Mo154 }	1584:1597	novel {Mo154 }-based systems	1584:1611	Overall, this work paves the way for the development of novel {Mo154 }-based systems, incorporated in self-healing and injectable hydrogels for dual chemo-photothermal therapy.
34382257	6	55	theme	Schiff	1011:1016	arg1	base					1018:1021	Schiff base	1011:1021	Schiff base	1011:1021	The gelation occurs within 60 s at room temperature, and the dual crosslinking by Schiff base and electrostatic interactions generates a dynamic network, which enables self-healing after injection.
34382257	9	56	theme	systemic	1509:1516	arg1	toxicity					1518:1525	no systemic toxicity	1506:1525	no systemic toxicity	1506:1525	This synergic system efficiently ablates the cancer tumor in vivo with no systemic toxicity.
34382257	2	57	theme	Mo154	355:359	arg1	agent					391:395	a promising photothermal agent	366:395	a promising photothermal agent due to its intervalence charge transfer transitions	366:447	In particular, the wheel-shaped cluster {Mo154 } is a promising photothermal agent due to its intervalence charge transfer transitions.
34382257	2	57	theme	Mo154	355:359	arg1	}					361:361	the wheel-shaped cluster {Mo154 }	329:361	the wheel-shaped cluster {Mo154 }	329:361	In particular, the wheel-shaped cluster {Mo154 } is a promising photothermal agent due to its intervalence charge transfer transitions.
34382257	10	58	theme	dual	1672:1675	arg1	therapy					1696:1702	dual chemo-photothermal therapy	1672:1702	dual chemo-photothermal therapy	1672:1702	Overall, this work paves the way for the development of novel {Mo154 }-based systems, incorporated in self-healing and injectable hydrogels for dual chemo-photothermal therapy.
34382257	10	59	theme	novel	1584:1588	arg1	}					1597:1597	novel {Mo154 }	1584:1597	novel {Mo154 }-based systems	1584:1611	Overall, this work paves the way for the development of novel {Mo154 }-based systems, incorporated in self-healing and injectable hydrogels for dual chemo-photothermal therapy.
34382257	7	60	theme	chemotherapeutic	1159:1174	arg1	drugs					1176:1180	chemotherapeutic drugs	1159:1180	chemotherapeutic drugs	1159:1180	Moreover, the hydrogel delivers chemotherapeutic drugs, with a release triggered by dual near infra-red (NIR) radiation and pH changes.
34382257	2	61	theme	{	354:354	arg1	agent					391:395	a promising photothermal agent	366:395	a promising photothermal agent due to its intervalence charge transfer transitions	366:447	In particular, the wheel-shaped cluster {Mo154 } is a promising photothermal agent due to its intervalence charge transfer transitions.
34382257	2	61	theme	{	354:354	arg1	}					361:361	the wheel-shaped cluster {Mo154 }	329:361	the wheel-shaped cluster {Mo154 }	329:361	In particular, the wheel-shaped cluster {Mo154 } is a promising photothermal agent due to its intervalence charge transfer transitions.
34382257	8	62	dep	along	1302:1306	arg1	with					1308:1311	with	1308:1311	with	1308:1311	This stimuli-responsive release system along with the photothermal conversion ability of the hydrogel allows the simultaneous combination of photothermal and chemotherapy.
34382257	2	63	theme	cluster	346:352	arg1	agent					391:395	a promising photothermal agent	366:395	a promising photothermal agent due to its intervalence charge transfer transitions	366:447	In particular, the wheel-shaped cluster {Mo154 } is a promising photothermal agent due to its intervalence charge transfer transitions.
34382257	2	63	theme	cluster	346:352	arg1	}					361:361	the wheel-shaped cluster {Mo154 }	329:361	the wheel-shaped cluster {Mo154 }	329:361	In particular, the wheel-shaped cluster {Mo154 } is a promising photothermal agent due to its intervalence charge transfer transitions.
34382257	8	64	theme	chemotherapy	1421:1432	arg1	combination					1389:1399	the simultaneous combination	1372:1399	the simultaneous combination of photothermal and chemotherapy	1372:1432	This stimuli-responsive release system along with the photothermal conversion ability of the hydrogel allows the simultaneous combination of photothermal and chemotherapy.
34382257	8	65	theme	simultaneous	1376:1387	arg1	combination					1389:1399	the simultaneous combination	1372:1399	the simultaneous combination of photothermal and chemotherapy	1372:1432	This stimuli-responsive release system along with the photothermal conversion ability of the hydrogel allows the simultaneous combination of photothermal and chemotherapy.
34382257	9	66	theme	cancer	1480:1485	arg1	tumor					1487:1491	the cancer tumor	1476:1491	the cancer tumor	1476:1491	This synergic system efficiently ablates the cancer tumor in vivo with no systemic toxicity.
34382257	1	67	theme	emerging	144:151	arg1	Polyoxometalates					120:135	Polyoxometalates	120:135	Polyoxometalates	120:135	Polyoxometalates are an emerging class of molecular clusters, with well-defined structures and chemical compositions that are produced through simple, low-cost, and highly reproducible methods.
34382257	1	67	theme	emerging	144:151	arg1	class					153:157	an emerging class	141:157	an emerging class of molecular clusters	141:179	Polyoxometalates are an emerging class of molecular clusters, with well-defined structures and chemical compositions that are produced through simple, low-cost, and highly reproducible methods.
34382257	8	68	theme	stimuli-responsive	1268:1285	arg1	system					1295:1300	This stimuli-responsive release system	1263:1300	This stimuli-responsive release system along with the photothermal conversion ability of the hydrogel	1263:1363	This stimuli-responsive release system along with the photothermal conversion ability of the hydrogel allows the simultaneous combination of photothermal and chemotherapy.
34382257	3	69	theme	systemic	484:491	arg1	administration					493:506	its systemic administration	480:506	its systemic administration	480:506	However, its toxicity hinders its systemic administration, being the development of a localized delivery system still incipient.
34382257	10	70	theme	systems	1605:1611	arg1	development					1569:1579	the development	1565:1579	the development of novel {Mo154 }-based systems, incorporated in self-healing and injectable hydrogels for dual chemo-photothermal therapy	1565:1702	Overall, this work paves the way for the development of novel {Mo154 }-based systems, incorporated in self-healing and injectable hydrogels for dual chemo-photothermal therapy.
34382257	0	71	with	}	55:55	arg1	pH					62:63	pH	62:63	pH	62:63	Dual-Crosslinked Dynamic Hydrogel Incorporating {Mo154 } with pH and NIR Responsiveness for Chemo-Photothermal Therapy.
34382257	0	71	with	}	55:55	arg1	Responsiveness					73:86	NIR Responsiveness	69:86	NIR Responsiveness	69:86	Dual-Crosslinked Dynamic Hydrogel Incorporating {Mo154 } with pH and NIR Responsiveness for Chemo-Photothermal Therapy.
34382257	2	72	theme	promising	368:376	arg1	agent					391:395	a promising photothermal agent	366:395	a promising photothermal agent due to its intervalence charge transfer transitions	366:447	In particular, the wheel-shaped cluster {Mo154 } is a promising photothermal agent due to its intervalence charge transfer transitions.
34382257	2	72	theme	promising	368:376	arg1	}					361:361	the wheel-shaped cluster {Mo154 }	329:361	the wheel-shaped cluster {Mo154 }	329:361	In particular, the wheel-shaped cluster {Mo154 } is a promising photothermal agent due to its intervalence charge transfer transitions.
34382257	5	73	theme	Mo154	920:924	arg1	}					926:926	{Mo154 }	919:926	{Mo154 }	919:926	The hydrogel is composed of benzylaldehyde functionalized polyethylene glycol, poly(N-isopropylacrylamide) functionalized chitosan and {Mo154 }.
34382257	4	74	theme	preparation	635:645	arg1	hydrogel					618:625	an injectable and self-healing hydrogel	587:625	an injectable and self-healing hydrogel of easy preparation and administration	587:664	Herein, an injectable and self-healing hydrogel of easy preparation and administration is developed, incorporating both {Mo154 } and doxorubicin for synergistic photothermal and chemotherapy applications.
34382257	6	75	theme	electrostatic	1027:1039	arg1	interactions					1041:1052	electrostatic interactions	1027:1052	electrostatic interactions	1027:1052	The gelation occurs within 60 s at room temperature, and the dual crosslinking by Schiff base and electrostatic interactions generates a dynamic network, which enables self-healing after injection.
34382257	8	76	theme	release	1287:1293	arg1	system					1295:1300	This stimuli-responsive release system	1263:1300	This stimuli-responsive release system along with the photothermal conversion ability of the hydrogel	1263:1363	This stimuli-responsive release system along with the photothermal conversion ability of the hydrogel allows the simultaneous combination of photothermal and chemotherapy.
34382257	4	77	theme	administration	651:664	arg1	hydrogel					618:625	an injectable and self-healing hydrogel	587:625	an injectable and self-healing hydrogel of easy preparation and administration	587:664	Herein, an injectable and self-healing hydrogel of easy preparation and administration is developed, incorporating both {Mo154 } and doxorubicin for synergistic photothermal and chemotherapy applications.
34382257	4	78	theme	Mo154	700:704	arg1	}					706:706	{Mo154 }	699:706	{Mo154 }	699:706	Herein, an injectable and self-healing hydrogel of easy preparation and administration is developed, incorporating both {Mo154 } and doxorubicin for synergistic photothermal and chemotherapy applications.
34382257	1	79	theme	simple	263:268	arg1	methods					305:311	simple, low-cost, and highly reproducible methods	263:311	simple, low-cost, and highly reproducible methods	263:311	Polyoxometalates are an emerging class of molecular clusters, with well-defined structures and chemical compositions that are produced through simple, low-cost, and highly reproducible methods.
34900436	6	0	theme	extracts	1030:1037	arg1	doses					1008:1012	increasing doses	997:1012	increasing doses of the prepared extracts	997:1037	Cell viability assays were performed on Caco-2 and IEC-6 intestinal epithelial cells using increasing doses of the prepared extracts.
34900436	3	1	theme	particular	445:454	arg1	nutrient					456:463	a particular nutrient	443:463	a particular nutrient for example antioxidants or omega-3 fatty acids	443:511	Typically, these 'superfoods' are rich in a particular nutrient for example antioxidants or omega-3 fatty acids.
34900436	4	2	theme	study	536:540	arg1	objective					518:526	The objective	514:526	The objective of this study	514:540	The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits.
34900436	7	3	theme	cell	1058:1061	arg1	viability					1063:1071	cell viability	1058:1071	cell viability	1058:1071	RESULTS Increased cell viability was observed in both cell lines with increasing concentrations of the flax seed, chia seed or hulled sunflower extracts (P < 0.05).
34900436	3	4	from	nutrient	456:463	arg1	rich					435:438	rich	435:438	rich	435:438	Typically, these 'superfoods' are rich in a particular nutrient for example antioxidants or omega-3 fatty acids.
34900436	2	5	theme	overall	262:268	arg1	health					270:275	overall health	262:275	overall health	262:275	'Superfoods' are claimed to prevent diseases as well as improving overall health, though the lack of explicit criteria means that any food can be labelled 'super' without support from scientific research.
34900436	4	6	theme	hulled	635:640	arg1	sunflower					642:650	hulled sunflower	635:650	hulled sunflower	635:650	The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits.
34900436	4	6	theme	hulled	635:640	arg1	properties					577:586	the nutritional properties	561:586	the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds	561:688	The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits.
34900436	5	7	theme	aqueous	783:789	arg1	method					802:807	a simple aqueous extraction method	774:807	a simple aqueous extraction method	774:807	METHODS We developed a simple aqueous extraction method for ground seeds and analysed their composition by mineral, protein and monosaccharide analyses.
34900436	1	8	used	used	127:130	arg2	superfoods					112:121	The term 'superfoods'	102:122	The term 'superfoods'	102:122	BACKGROUND The term 'superfoods' is used to market foods considered to have significant health benefits.
34900436	4	9	theme	seeds	616:620	arg1	selection					593:601	a selection	591:601	a selection of superfood seeds	591:620	The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits.
34900436	3	10	from	rich	435:438	arg1	nutrient					456:463	a particular nutrient	443:463	a particular nutrient for example antioxidants or omega-3 fatty acids	443:511	Typically, these 'superfoods' are rich in a particular nutrient for example antioxidants or omega-3 fatty acids.
34900436	1	11	theme	health	179:184	arg1	benefits					186:193	significant health benefits	167:193	significant health benefits	167:193	BACKGROUND The term 'superfoods' is used to market foods considered to have significant health benefits.
34900436	7	12	theme	sunflower	1174:1182	arg1	extracts					1184:1191	hulled sunflower extracts	1167:1191	hulled sunflower extracts (P < 0.05)	1167:1202	RESULTS Increased cell viability was observed in both cell lines with increasing concentrations of the flax seed, chia seed or hulled sunflower extracts (P < 0.05).
34900436	7	12	theme	sunflower	1174:1182	arg1	<					1196:1196	P < 0.05	1194:1201	P < 0.05	1194:1201	RESULTS Increased cell viability was observed in both cell lines with increasing concentrations of the flax seed, chia seed or hulled sunflower extracts (P < 0.05).
34900436	8	13	theme	antioxidant	1379:1389	arg1	activity					1391:1398	the highest antioxidant activity	1367:1398	the highest antioxidant activity	1367:1398	Compositional analyses revealed the presence of polysaccharides, proteins and essential minerals in the aqueous extracts and in vitro assays showed sunflower had the highest antioxidant activity.
34900436	8	14	theme	Compositional	1205:1217	arg1	analyses					1219:1226	Compositional analyses	1205:1226	Compositional analyses	1205:1226	Compositional analyses revealed the presence of polysaccharides, proteins and essential minerals in the aqueous extracts and in vitro assays showed sunflower had the highest antioxidant activity.
34900436	8	15	attach	presence	1241:1248	arg2	polysaccharides					1253:1267	polysaccharides	1253:1267	polysaccharides	1253:1267	Compositional analyses revealed the presence of polysaccharides, proteins and essential minerals in the aqueous extracts and in vitro assays showed sunflower had the highest antioxidant activity.
34900436	8	15	attach	presence	1241:1248	arg1	extracts					1317:1324	the aqueous extracts	1305:1324	the aqueous extracts	1305:1324	Compositional analyses revealed the presence of polysaccharides, proteins and essential minerals in the aqueous extracts and in vitro assays showed sunflower had the highest antioxidant activity.
34900436	8	15	attach	presence	1241:1248	arg2	minerals					1293:1300	essential minerals	1283:1300	essential minerals	1283:1300	Compositional analyses revealed the presence of polysaccharides, proteins and essential minerals in the aqueous extracts and in vitro assays showed sunflower had the highest antioxidant activity.
34900436	8	15	attach	presence	1241:1248	arg2	proteins					1270:1277	proteins	1270:1277	proteins	1270:1277	Compositional analyses revealed the presence of polysaccharides, proteins and essential minerals in the aqueous extracts and in vitro assays showed sunflower had the highest antioxidant activity.
34900436	9	16	theme	extract	1425:1431	arg1	composition					1433:1443	extract composition	1425:1443	extract composition	1425:1443	However, differences in extract composition and antioxidant properties could not be directly related to the observed increase in cell viability suggesting that other components in the extracts may be responsible.
34900436	9	17	from	increase	1518:1525	arg1	viability					1535:1543	cell viability	1530:1543	cell viability	1530:1543	However, differences in extract composition and antioxidant properties could not be directly related to the observed increase in cell viability suggesting that other components in the extracts may be responsible.
34900436	4	18	theme	processed	669:677	arg1	seeds					684:688	processed hemp seeds	669:688	processed hemp seeds	669:688	The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits.
34900436	0	19	theme	potential	64:72	arg1	benefits					81:88	their potential health benefits	58:88	their potential health benefits	58:88	Nutritional properties of selected superfood extracts and their potential health benefits.
34900436	8	20	dep	in	1330:1331	arg1	vitro					1333:1337	vitro	1333:1337	vitro	1333:1337	Compositional analyses revealed the presence of polysaccharides, proteins and essential minerals in the aqueous extracts and in vitro assays showed sunflower had the highest antioxidant activity.
34900436	1	21	dep	BACKGROUND	91:100	arg1	used					127:130	used	127:130	is used to market foods considered to have significant health benefits	124:193	BACKGROUND The term 'superfoods' is used to market foods considered to have significant health benefits.
34900436	0	22	theme	benefits	81:88	arg1	properties					12:21	Nutritional properties	0:21	Nutritional properties of selected superfood extracts and their potential health benefits	0:88	Nutritional properties of selected superfood extracts and their potential health benefits.
34900436	4	23	theme	seeds	684:688	arg1	properties					577:586	the nutritional properties	561:586	the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds	561:688	The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits.
34900436	4	23	theme	seeds	684:688	arg1	chia					629:632	chia	629:632	chia	629:632	The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits.
34900436	4	23	theme	seeds	684:688	arg1	sunflower					642:650	hulled sunflower	635:650	hulled sunflower	635:650	The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits.
34900436	4	23	theme	seeds	684:688	arg1	flax					623:626	flax	623:626	flax	623:626	The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits.
34900436	4	23	theme	seeds	684:688	arg1	types					660:664	two types	656:664	two types of processed hemp seeds	656:688	The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits.
34900436	7	24	theme	seed	1148:1151	arg1	flax					1143:1146	the flax seed	1139:1151	the flax seed	1139:1151	RESULTS Increased cell viability was observed in both cell lines with increasing concentrations of the flax seed, chia seed or hulled sunflower extracts (P < 0.05).
34900436	3	25	theme	omega-3	493:499	arg1	acids					507:511	omega-3 fatty acids	493:511	omega-3 fatty acids	493:511	Typically, these 'superfoods' are rich in a particular nutrient for example antioxidants or omega-3 fatty acids.
34900436	6	26	theme	viability	911:919	arg1	assays					921:926	Cell viability assays	906:926	Cell viability assays	906:926	Cell viability assays were performed on Caco-2 and IEC-6 intestinal epithelial cells using increasing doses of the prepared extracts.
34900436	8	27	theme	essential	1283:1291	arg1	minerals					1293:1300	essential minerals	1283:1300	essential minerals	1283:1300	Compositional analyses revealed the presence of polysaccharides, proteins and essential minerals in the aqueous extracts and in vitro assays showed sunflower had the highest antioxidant activity.
34900436	0	28	theme	health	74:79	arg1	benefits					81:88	their potential health benefits	58:88	their potential health benefits	58:88	Nutritional properties of selected superfood extracts and their potential health benefits.
34900436	0	29	theme	Nutritional	0:10	arg1	properties					12:21	Nutritional properties	0:21	Nutritional properties of selected superfood extracts and their potential health benefits	0:88	Nutritional properties of selected superfood extracts and their potential health benefits.
34900436	8	30	from	presence	1241:1248	arg1	extracts					1317:1324	the aqueous extracts	1305:1324	the aqueous extracts	1305:1324	Compositional analyses revealed the presence of polysaccharides, proteins and essential minerals in the aqueous extracts and in vitro assays showed sunflower had the highest antioxidant activity.
34900436	9	31	theme	observed	1509:1516	arg1	increase					1518:1525	the observed increase	1505:1525	the observed increase in cell viability	1505:1543	However, differences in extract composition and antioxidant properties could not be directly related to the observed increase in cell viability suggesting that other components in the extracts may be responsible.
34900436	6	32	theme	increasing	997:1006	arg1	doses					1008:1012	increasing doses	997:1012	increasing doses of the prepared extracts	997:1037	Cell viability assays were performed on Caco-2 and IEC-6 intestinal epithelial cells using increasing doses of the prepared extracts.
34900436	8	33	theme	aqueous	1309:1315	arg1	extracts					1317:1324	the aqueous extracts	1305:1324	the aqueous extracts	1305:1324	Compositional analyses revealed the presence of polysaccharides, proteins and essential minerals in the aqueous extracts and in vitro assays showed sunflower had the highest antioxidant activity.
34900436	4	34	theme	health	736:741	arg1	benefits					743:750	potential health benefits	726:750	potential health benefits	726:750	The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits.
34900436	10	35	theme	Future	1614:1619	arg1	studies					1621:1627	Future studies	1614:1627	Future studies	1614:1627	Future studies will further characterize these extracts and investigate whether they are beneficial for gastrointestinal health.
34900436	7	36	theme	cell	1094:1097	arg1	lines					1099:1103	both cell lines	1089:1103	both cell lines	1089:1103	RESULTS Increased cell viability was observed in both cell lines with increasing concentrations of the flax seed, chia seed or hulled sunflower extracts (P < 0.05).
34900436	0	37	theme	superfood	35:43	arg1	extracts					45:52	selected superfood extracts	26:52	selected superfood extracts	26:52	Nutritional properties of selected superfood extracts and their potential health benefits.
34900436	6	38	theme	intestinal	963:972	arg1	cells					985:989	IEC-6 intestinal epithelial cells	957:989	IEC-6 intestinal epithelial cells	957:989	Cell viability assays were performed on Caco-2 and IEC-6 intestinal epithelial cells using increasing doses of the prepared extracts.
34900436	2	39	theme	explicit	297:304	arg1	criteria					306:313	explicit criteria	297:313	explicit criteria	297:313	'Superfoods' are claimed to prevent diseases as well as improving overall health, though the lack of explicit criteria means that any food can be labelled 'super' without support from scientific research.
34900436	5	40	theme	ground	813:818	arg1	seeds					820:824	ground seeds	813:824	ground seeds	813:824	METHODS We developed a simple aqueous extraction method for ground seeds and analysed their composition by mineral, protein and monosaccharide analyses.
34900436	5	41	dep	METHODS	753:759	arg1	developed					764:772	developed	764:772	developed a simple aqueous extraction method for ground seeds	764:824	METHODS We developed a simple aqueous extraction method for ground seeds and analysed their composition by mineral, protein and monosaccharide analyses.
34900436	5	41	dep	METHODS	753:759	arg1	analysed					830:837	analysed	830:837	analysed their composition by mineral, protein and monosaccharide analyses	830:903	METHODS We developed a simple aqueous extraction method for ground seeds and analysed their composition by mineral, protein and monosaccharide analyses.
34900436	6	42	theme	prepared	1021:1028	arg1	extracts					1030:1037	the prepared extracts	1017:1037	the prepared extracts	1017:1037	Cell viability assays were performed on Caco-2 and IEC-6 intestinal epithelial cells using increasing doses of the prepared extracts.
34900436	10	43	theme	gastrointestinal	1718:1733	arg1	health					1735:1740	gastrointestinal health	1718:1740	gastrointestinal health	1718:1740	Future studies will further characterize these extracts and investigate whether they are beneficial for gastrointestinal health.
34900436	8	44	theme	polysaccharides	1253:1267	arg1	presence					1241:1248	the presence	1237:1248	the presence of polysaccharides, proteins and essential minerals in the aqueous extracts	1237:1324	Compositional analyses revealed the presence of polysaccharides, proteins and essential minerals in the aqueous extracts and in vitro assays showed sunflower had the highest antioxidant activity.
34900436	7	45	located	observed	1077:1084	arg2	viability					1063:1071	cell viability	1058:1071	cell viability	1058:1071	RESULTS Increased cell viability was observed in both cell lines with increasing concentrations of the flax seed, chia seed or hulled sunflower extracts (P < 0.05).
34900436	7	45	located	observed	1077:1084	arg1	lines					1099:1103	both cell lines	1089:1103	both cell lines	1089:1103	RESULTS Increased cell viability was observed in both cell lines with increasing concentrations of the flax seed, chia seed or hulled sunflower extracts (P < 0.05).
34900436	3	46	theme	example	469:475	arg1	antioxidants					477:488	example antioxidants	469:488	example antioxidants	469:488	Typically, these 'superfoods' are rich in a particular nutrient for example antioxidants or omega-3 fatty acids.
34900436	5	47	theme	extraction	791:800	arg1	method					802:807	a simple aqueous extraction method	774:807	a simple aqueous extraction method	774:807	METHODS We developed a simple aqueous extraction method for ground seeds and analysed their composition by mineral, protein and monosaccharide analyses.
34900436	5	48	theme	simple	776:781	arg1	method					802:807	a simple aqueous extraction method	774:807	a simple aqueous extraction method	774:807	METHODS We developed a simple aqueous extraction method for ground seeds and analysed their composition by mineral, protein and monosaccharide analyses.
34900436	4	49	theme	selection	593:601	arg1	chia					629:632	chia	629:632	chia	629:632	The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits.
34900436	4	49	theme	selection	593:601	arg1	sunflower					642:650	hulled sunflower	635:650	hulled sunflower	635:650	The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits.
34900436	4	49	theme	selection	593:601	arg1	properties					577:586	the nutritional properties	561:586	the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds	561:688	The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits.
34900436	4	49	theme	selection	593:601	arg1	flax					623:626	flax	623:626	flax	623:626	The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits.
34900436	7	50	theme	chia	1154:1157	arg1	seed					1159:1162	chia seed	1154:1162	chia seed	1154:1162	RESULTS Increased cell viability was observed in both cell lines with increasing concentrations of the flax seed, chia seed or hulled sunflower extracts (P < 0.05).
34900436	1	51	theme	significant	167:177	arg1	benefits					186:193	significant health benefits	167:193	significant health benefits	167:193	BACKGROUND The term 'superfoods' is used to market foods considered to have significant health benefits.
34900436	6	52	theme	Cell	906:909	arg1	assays					921:926	Cell viability assays	906:926	Cell viability assays	906:926	Cell viability assays were performed on Caco-2 and IEC-6 intestinal epithelial cells using increasing doses of the prepared extracts.
34900436	4	53	theme	superfood	606:614	arg1	seeds					616:620	superfood seeds	606:620	superfood seeds	606:620	The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits.
34900436	3	54	theme	fatty	501:505	arg1	acids					507:511	omega-3 fatty acids	493:511	omega-3 fatty acids	493:511	Typically, these 'superfoods' are rich in a particular nutrient for example antioxidants or omega-3 fatty acids.
34900436	8	55	theme	highest	1371:1377	arg1	activity					1391:1398	the highest antioxidant activity	1367:1398	the highest antioxidant activity	1367:1398	Compositional analyses revealed the presence of polysaccharides, proteins and essential minerals in the aqueous extracts and in vitro assays showed sunflower had the highest antioxidant activity.
34900436	4	56	theme	nutritional	565:575	arg1	chia					629:632	chia	629:632	chia	629:632	The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits.
34900436	4	56	theme	nutritional	565:575	arg1	sunflower					642:650	hulled sunflower	635:650	hulled sunflower	635:650	The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits.
34900436	4	56	theme	nutritional	565:575	arg1	properties					577:586	the nutritional properties	561:586	the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds	561:688	The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits.
34900436	4	56	theme	nutritional	565:575	arg1	flax					623:626	flax	623:626	flax	623:626	The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits.
34900436	1	57	theme	term	106:109	arg1	superfoods					112:121	The term 'superfoods'	102:122	The term 'superfoods'	102:122	BACKGROUND The term 'superfoods' is used to market foods considered to have significant health benefits.
34900436	2	58	dep	claimed	213:219	arg1	Superfoods					197:206	Superfoods	197:206	Superfoods	197:206	'Superfoods' are claimed to prevent diseases as well as improving overall health, though the lack of explicit criteria means that any food can be labelled 'super' without support from scientific research.
34900436	8	59	dep	showed	1346:1351	arg1	had					1363:1365	had	1363:1365	showed sunflower had the highest antioxidant activity	1346:1398	Compositional analyses revealed the presence of polysaccharides, proteins and essential minerals in the aqueous extracts and in vitro assays showed sunflower had the highest antioxidant activity.
34900436	6	60	theme	IEC-6	957:961	arg1	cells					985:989	IEC-6 intestinal epithelial cells	957:989	IEC-6 intestinal epithelial cells	957:989	Cell viability assays were performed on Caco-2 and IEC-6 intestinal epithelial cells using increasing doses of the prepared extracts.
34900436	8	61	theme	proteins	1270:1277	arg1	presence					1241:1248	the presence	1237:1248	the presence of polysaccharides, proteins and essential minerals in the aqueous extracts	1237:1324	Compositional analyses revealed the presence of polysaccharides, proteins and essential minerals in the aqueous extracts and in vitro assays showed sunflower had the highest antioxidant activity.
34900436	4	62	contain	have	721:724	arg2	benefits					743:750	potential health benefits	726:750	potential health benefits	726:750	The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits.
34900436	4	62	contain	have	721:724	arg1	they					712:715	they	712:715	they	712:715	The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits.
34900436	7	63	theme	seed	1159:1162	arg1	concentrations					1121:1134	increasing concentrations	1110:1134	increasing concentrations of the flax seed, chia seed or hulled sunflower extracts (P < 0.05)	1110:1202	RESULTS Increased cell viability was observed in both cell lines with increasing concentrations of the flax seed, chia seed or hulled sunflower extracts (P < 0.05).
34900436	9	64	theme	antioxidant	1449:1459	arg1	properties					1461:1470	antioxidant properties	1449:1470	antioxidant properties	1449:1470	However, differences in extract composition and antioxidant properties could not be directly related to the observed increase in cell viability suggesting that other components in the extracts may be responsible.
34900436	2	65	from	research	391:398	arg1	support					367:373	support	367:373	support from scientific research	367:398	'Superfoods' are claimed to prevent diseases as well as improving overall health, though the lack of explicit criteria means that any food can be labelled 'super' without support from scientific research.
34900436	7	66	theme	flax	1143:1146	arg1	concentrations					1121:1134	increasing concentrations	1110:1134	increasing concentrations of the flax seed, chia seed or hulled sunflower extracts (P < 0.05)	1110:1202	RESULTS Increased cell viability was observed in both cell lines with increasing concentrations of the flax seed, chia seed or hulled sunflower extracts (P < 0.05).
34900436	5	67	theme	monosaccharide	881:894	arg1	analyses					896:903	mineral, protein and monosaccharide analyses	860:903	mineral, protein and monosaccharide analyses	860:903	METHODS We developed a simple aqueous extraction method for ground seeds and analysed their composition by mineral, protein and monosaccharide analyses.
34900436	4	68	theme	hemp	679:682	arg1	seeds					684:688	processed hemp seeds	669:688	processed hemp seeds	669:688	The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits.
34900436	7	69	theme	increasing	1110:1119	arg1	concentrations					1121:1134	increasing concentrations	1110:1134	increasing concentrations of the flax seed, chia seed or hulled sunflower extracts (P < 0.05)	1110:1202	RESULTS Increased cell viability was observed in both cell lines with increasing concentrations of the flax seed, chia seed or hulled sunflower extracts (P < 0.05).
34900436	5	70	theme	mineral	860:866	arg1	analyses					896:903	mineral, protein and monosaccharide analyses	860:903	mineral, protein and monosaccharide analyses	860:903	METHODS We developed a simple aqueous extraction method for ground seeds and analysed their composition by mineral, protein and monosaccharide analyses.
34900436	9	71	from	components	1567:1576	arg1	extracts					1585:1592	the extracts	1581:1592	the extracts	1581:1592	However, differences in extract composition and antioxidant properties could not be directly related to the observed increase in cell viability suggesting that other components in the extracts may be responsible.
34900436	8	72	theme	minerals	1293:1300	arg1	presence					1241:1248	the presence	1237:1248	the presence of polysaccharides, proteins and essential minerals in the aqueous extracts	1237:1324	Compositional analyses revealed the presence of polysaccharides, proteins and essential minerals in the aqueous extracts and in vitro assays showed sunflower had the highest antioxidant activity.
34900436	0	73	theme	selected	26:33	arg1	extracts					45:52	selected superfood extracts	26:52	selected superfood extracts	26:52	Nutritional properties of selected superfood extracts and their potential health benefits.
34900436	5	74	theme	protein	869:875	arg1	analyses					896:903	mineral, protein and monosaccharide analyses	860:903	mineral, protein and monosaccharide analyses	860:903	METHODS We developed a simple aqueous extraction method for ground seeds and analysed their composition by mineral, protein and monosaccharide analyses.
34900436	2	75	theme	scientific	380:389	arg1	research					391:398	scientific research	380:398	scientific research	380:398	'Superfoods' are claimed to prevent diseases as well as improving overall health, though the lack of explicit criteria means that any food can be labelled 'super' without support from scientific research.
34900436	0	76	theme	extracts	45:52	arg1	properties					12:21	Nutritional properties	0:21	Nutritional properties of selected superfood extracts and their potential health benefits	0:88	Nutritional properties of selected superfood extracts and their potential health benefits.
34900436	9	77	theme	cell	1530:1533	arg1	viability					1535:1543	cell viability	1530:1543	cell viability	1530:1543	However, differences in extract composition and antioxidant properties could not be directly related to the observed increase in cell viability suggesting that other components in the extracts may be responsible.
34900436	6	78	theme	epithelial	974:983	arg1	cells					985:989	IEC-6 intestinal epithelial cells	957:989	IEC-6 intestinal epithelial cells	957:989	Cell viability assays were performed on Caco-2 and IEC-6 intestinal epithelial cells using increasing doses of the prepared extracts.
34900436	4	79	theme	potential	726:734	arg1	benefits					743:750	potential health benefits	726:750	potential health benefits	726:750	The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits.
34900436	4	80	dep	properties	577:586	arg1	properties					577:586	the nutritional properties	561:586	the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds	561:688	The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits.
34900436	4	80	dep	properties	577:586	arg1	chia					629:632	chia	629:632	chia	629:632	The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits.
34900436	4	80	dep	properties	577:586	arg1	sunflower					642:650	hulled sunflower	635:650	hulled sunflower	635:650	The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits.
34900436	4	80	dep	properties	577:586	arg1	flax					623:626	flax	623:626	flax	623:626	The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits.
34900436	4	80	dep	properties	577:586	arg1	types					660:664	two types	656:664	two types of processed hemp seeds	656:688	The objective of this study was to investigate the nutritional properties of a selection of superfood seeds: flax, chia, hulled sunflower and two types of processed hemp seeds and determine whether they may have potential health benefits.
34900436	9	81	from	differences	1410:1420	arg1	composition					1433:1443	extract composition	1425:1443	extract composition	1425:1443	However, differences in extract composition and antioxidant properties could not be directly related to the observed increase in cell viability suggesting that other components in the extracts may be responsible.
34900436	9	81	from	differences	1410:1420	arg1	properties					1461:1470	antioxidant properties	1449:1470	antioxidant properties	1449:1470	However, differences in extract composition and antioxidant properties could not be directly related to the observed increase in cell viability suggesting that other components in the extracts may be responsible.
34900436	8	82	theme	in	1330:1331	arg1	assays					1339:1344	in vitro assays	1330:1344	in vitro assays	1330:1344	Compositional analyses revealed the presence of polysaccharides, proteins and essential minerals in the aqueous extracts and in vitro assays showed sunflower had the highest antioxidant activity.
34900436	2	83	theme	criteria	306:313	arg1	lack					289:292	the lack	285:292	the lack of explicit criteria	285:313	'Superfoods' are claimed to prevent diseases as well as improving overall health, though the lack of explicit criteria means that any food can be labelled 'super' without support from scientific research.
34900436	9	84	theme	other	1561:1565	arg1	components					1567:1576	other components	1561:1576	other components in the extracts	1561:1592	However, differences in extract composition and antioxidant properties could not be directly related to the observed increase in cell viability suggesting that other components in the extracts may be responsible.
34900436	7	85	theme	P	1194:1194	arg1	extracts					1184:1191	hulled sunflower extracts	1167:1191	hulled sunflower extracts (P < 0.05)	1167:1202	RESULTS Increased cell viability was observed in both cell lines with increasing concentrations of the flax seed, chia seed or hulled sunflower extracts (P < 0.05).
34900436	7	85	theme	P	1194:1194	arg1	<					1196:1196	P < 0.05	1194:1201	P < 0.05	1194:1201	RESULTS Increased cell viability was observed in both cell lines with increasing concentrations of the flax seed, chia seed or hulled sunflower extracts (P < 0.05).
34900436	7	86	theme	extracts	1184:1191	arg1	concentrations					1121:1134	increasing concentrations	1110:1134	increasing concentrations of the flax seed, chia seed or hulled sunflower extracts (P < 0.05)	1110:1202	RESULTS Increased cell viability was observed in both cell lines with increasing concentrations of the flax seed, chia seed or hulled sunflower extracts (P < 0.05).
34900436	7	87	theme	hulled	1167:1172	arg1	extracts					1184:1191	hulled sunflower extracts	1167:1191	hulled sunflower extracts (P < 0.05)	1167:1202	RESULTS Increased cell viability was observed in both cell lines with increasing concentrations of the flax seed, chia seed or hulled sunflower extracts (P < 0.05).
34900436	7	87	theme	hulled	1167:1172	arg1	<					1196:1196	P < 0.05	1194:1201	P < 0.05	1194:1201	RESULTS Increased cell viability was observed in both cell lines with increasing concentrations of the flax seed, chia seed or hulled sunflower extracts (P < 0.05).
32779675	7	0	from	difference	1228:1237	arg1	composition					1246:1256	the composition	1242:1256	the composition of hepatic metabolic profiles	1242:1286	Principal component analysis and partial least-squares discriminant analysis showed a dramatic difference in the composition of hepatic metabolic profiles between the two groups.
32779675	10	1	theme	mitochondrial	1592:1604	arg1	capacity					1618:1625	the hepatic mitochondrial antioxidant capacity	1580:1625	the hepatic mitochondrial antioxidant capacity	1580:1625	Additionally, with the accumulation of antioxidant-related metabolites in the GOS piglet liver, the hepatic mitochondrial antioxidant capacity increased, and the phosphorylation of AMP-activated protein kinase and the protein expression levels of nuclear factor-erythroid-2-related factor 2 and heme oxygenase-1 were also upregulated in the liver.
32779675	1	2	theme	human	262:266	arg1	development					247:257	the development	243:257	the development of human and animal neonates	243:286	The suckling stage is a critical window time for the development of human and animal neonates.
32779675	4	3	theme	kg	818:819	arg1	weight					826:831	kg body weight	818:831	kg body weight per day	818:839	In the first week after birth, the piglets in the GOS and control (CON) groups were fed with GOS solution (10 mL for 1 g per kg body weight per day) and physiological saline, respectively.
32779675	1	4	theme	animal	272:277	arg1	development					247:257	the development	243:257	the development of human and animal neonates	243:286	The suckling stage is a critical window time for the development of human and animal neonates.
32779675	3	5	theme	present	541:547	arg1	study					549:553	the present study	537:553	the present study	537:553	In the present study, sixty neonatal piglets were used to investigate the effects of early-life GOS intervention on the hepatic metabolic profiles of piglets.
32779675	10	6	theme	protein	1679:1685	arg1	kinase					1687:1692	AMP-activated protein kinase	1665:1692	AMP-activated protein kinase	1665:1692	Additionally, with the accumulation of antioxidant-related metabolites in the GOS piglet liver, the hepatic mitochondrial antioxidant capacity increased, and the phosphorylation of AMP-activated protein kinase and the protein expression levels of nuclear factor-erythroid-2-related factor 2 and heme oxygenase-1 were also upregulated in the liver.
32779675	7	7	theme	metabolic	1269:1277	arg1	profiles					1279:1286	hepatic metabolic profiles	1261:1286	hepatic metabolic profiles	1261:1286	Principal component analysis and partial least-squares discriminant analysis showed a dramatic difference in the composition of hepatic metabolic profiles between the two groups.
32779675	6	8	theme	cholesterol	1032:1042	arg1	concentration					1044:1056	a lower total serum cholesterol concentration	1012:1056	a lower total serum cholesterol concentration on day 8	1012:1065	Compared with the CON piglets, the GOS piglets showed a lower total serum cholesterol concentration on day 8, but a higher concentration of serum HDL-C and albumin on day 21.
32779675	3	9	theme	neonatal	562:569	arg1	piglets					571:577	sixty neonatal piglets	556:577	sixty neonatal piglets	556:577	In the present study, sixty neonatal piglets were used to investigate the effects of early-life GOS intervention on the hepatic metabolic profiles of piglets.
32779675	7	10	theme	profiles	1279:1286	arg1	composition					1246:1256	the composition	1242:1256	the composition of hepatic metabolic profiles	1242:1286	Principal component analysis and partial least-squares discriminant analysis showed a dramatic difference in the composition of hepatic metabolic profiles between the two groups.
32779675	0	11	theme	signaling	150:158	arg1	pathway					160:166	the AMPK/Nrf2 signaling pathway	136:166	the AMPK/Nrf2 signaling pathway in suckling piglet liver	136:191	Early galactooligosaccharide intervention alters the metabolic profile, improves the antioxidant capacity of mitochondria and activates the AMPK/Nrf2 signaling pathway in suckling piglet liver.
32779675	10	12	theme	protein	1702:1708	arg1	levels					1721:1726	the protein expression levels	1698:1726	the protein expression levels of nuclear factor-erythroid-2-related factor 2 and heme oxygenase-1	1698:1794	Additionally, with the accumulation of antioxidant-related metabolites in the GOS piglet liver, the hepatic mitochondrial antioxidant capacity increased, and the phosphorylation of AMP-activated protein kinase and the protein expression levels of nuclear factor-erythroid-2-related factor 2 and heme oxygenase-1 were also upregulated in the liver.
32779675	1	13	theme	window	227:232	arg1	time					234:237	a critical window time	216:237	a critical window time for the development of human and animal neonates	216:286	The suckling stage is a critical window time for the development of human and animal neonates.
32779675	1	13	theme	window	227:232	arg1	stage					207:211	The suckling stage	194:211	The suckling stage	194:211	The suckling stage is a critical window time for the development of human and animal neonates.
32779675	7	14	theme	hepatic	1261:1267	arg1	profiles					1279:1286	hepatic metabolic profiles	1261:1286	hepatic metabolic profiles	1261:1286	Principal component analysis and partial least-squares discriminant analysis showed a dramatic difference in the composition of hepatic metabolic profiles between the two groups.
32779675	8	15	theme	amino	1363:1367	arg1	metabolism					1374:1383	the lipid and amino acid metabolism	1349:1383	metabolism	1374:1383	The GOS intervention mainly affected the lipid and amino acid metabolism of the piglets.
32779675	0	16	theme	piglet	180:185	arg1	liver					187:191	suckling piglet liver	171:191	suckling piglet liver	171:191	Early galactooligosaccharide intervention alters the metabolic profile, improves the antioxidant capacity of mitochondria and activates the AMPK/Nrf2 signaling pathway in suckling piglet liver.
32779675	3	17	theme	GOS	630:632	arg1	intervention					634:645	early-life GOS intervention	619:645	early-life GOS intervention	619:645	In the present study, sixty neonatal piglets were used to investigate the effects of early-life GOS intervention on the hepatic metabolic profiles of piglets.
32779675	1	18	dep	human	262:266	arg1	neonates					279:286	neonates	279:286	neonates	279:286	The suckling stage is a critical window time for the development of human and animal neonates.
32779675	6	19	theme	higher	1074:1079	arg1	concentration					1081:1093	a higher concentration	1072:1093	a higher concentration of serum HDL-C and albumin	1072:1120	Compared with the CON piglets, the GOS piglets showed a lower total serum cholesterol concentration on day 8, but a higher concentration of serum HDL-C and albumin on day 21.
32779675	2	20	theme	treatment	494:502	arg1	groups					504:509	different treatment groups	484:509	different treatment groups	484:509	Our previous study found that an early-life galactooligosaccharide (GOS) intervention could improve the growth status of suckling piglets; however, the difference in the metabolic status between different treatment groups still remains unclear.
32779675	4	21	theme	first	700:704	arg1	week					706:709	the first week	696:709	the first week after birth	696:721	In the first week after birth, the piglets in the GOS and control (CON) groups were fed with GOS solution (10 mL for 1 g per kg body weight per day) and physiological saline, respectively.
32779675	0	22	theme	antioxidant	85:95	arg1	capacity					97:104	the antioxidant capacity	81:104	the antioxidant capacity of mitochondria	81:120	Early galactooligosaccharide intervention alters the metabolic profile, improves the antioxidant capacity of mitochondria and activates the AMPK/Nrf2 signaling pathway in suckling piglet liver.
32779675	2	23	from	difference	441:450	arg1	status					469:474	the metabolic status	455:474	the metabolic status	455:474	Our previous study found that an early-life galactooligosaccharide (GOS) intervention could improve the growth status of suckling piglets; however, the difference in the metabolic status between different treatment groups still remains unclear.
32779675	9	24	theme	CPT-1	1431:1435	arg1	PCG1α					1441:1445	PCG1α	1441:1445	PCG1α	1441:1445	Meanwhile, the mRNA levels of CPT-1 and PCG1α increased in the GOS piglets' liver.
32779675	9	24	theme	CPT-1	1431:1435	arg1	Meanwhile					1401:1409	Meanwhile	1401:1409	Meanwhile	1401:1409	Meanwhile, the mRNA levels of CPT-1 and PCG1α increased in the GOS piglets' liver.
32779675	9	24	theme	CPT-1	1431:1435	arg1	levels					1421:1426	the mRNA levels	1412:1426	the mRNA levels of CPT-1	1412:1435	Meanwhile, the mRNA levels of CPT-1 and PCG1α increased in the GOS piglets' liver.
32779675	10	25	theme	metabolites	1543:1553	arg1	accumulation					1507:1518	the accumulation	1503:1518	the accumulation of antioxidant-related metabolites in the GOS piglet liver	1503:1577	Additionally, with the accumulation of antioxidant-related metabolites in the GOS piglet liver, the hepatic mitochondrial antioxidant capacity increased, and the phosphorylation of AMP-activated protein kinase and the protein expression levels of nuclear factor-erythroid-2-related factor 2 and heme oxygenase-1 were also upregulated in the liver.
32779675	6	26	theme	CON	976:978	arg1	piglets					980:986	the CON piglets	972:986	the CON piglets	972:986	Compared with the CON piglets, the GOS piglets showed a lower total serum cholesterol concentration on day 8, but a higher concentration of serum HDL-C and albumin on day 21.
32779675	2	27	theme	growth	393:398	arg1	status					400:405	the growth status	389:405	the growth status of suckling piglets	389:425	Our previous study found that an early-life galactooligosaccharide (GOS) intervention could improve the growth status of suckling piglets; however, the difference in the metabolic status between different treatment groups still remains unclear.
32779675	0	28	theme	Early	0:4	arg1	intervention					29:40	Early galactooligosaccharide intervention	0:40	Early galactooligosaccharide intervention	0:40	Early galactooligosaccharide intervention alters the metabolic profile, improves the antioxidant capacity of mitochondria and activates the AMPK/Nrf2 signaling pathway in suckling piglet liver.
32779675	4	29	from	piglets	728:734	arg1	groups					765:770	the GOS and control (CON) groups	739:770	the GOS and control (CON) groups	739:770	In the first week after birth, the piglets in the GOS and control (CON) groups were fed with GOS solution (10 mL for 1 g per kg body weight per day) and physiological saline, respectively.
32779675	10	30	theme	piglet	1566:1571	arg1	liver					1573:1577	the GOS piglet liver	1558:1577	the GOS piglet liver	1558:1577	Additionally, with the accumulation of antioxidant-related metabolites in the GOS piglet liver, the hepatic mitochondrial antioxidant capacity increased, and the phosphorylation of AMP-activated protein kinase and the protein expression levels of nuclear factor-erythroid-2-related factor 2 and heme oxygenase-1 were also upregulated in the liver.
32779675	9	31	theme	GOS	1464:1466	arg1	piglets					1468:1474	the GOS piglets'	1460:1475	the GOS piglets' liver	1460:1481	Meanwhile, the mRNA levels of CPT-1 and PCG1α increased in the GOS piglets' liver.
32779675	3	32	theme	hepatic	654:660	arg1	profiles					672:679	the hepatic metabolic profiles	650:679	the hepatic metabolic profiles of piglets	650:690	In the present study, sixty neonatal piglets were used to investigate the effects of early-life GOS intervention on the hepatic metabolic profiles of piglets.
32779675	6	33	from	concentration	1044:1056	arg1	day					1061:1063	day 8	1061:1065	day 8	1061:1065	Compared with the CON piglets, the GOS piglets showed a lower total serum cholesterol concentration on day 8, but a higher concentration of serum HDL-C and albumin on day 21.
32779675	2	34	theme	piglets	419:425	arg1	status					400:405	the growth status	389:405	the growth status of suckling piglets	389:425	Our previous study found that an early-life galactooligosaccharide (GOS) intervention could improve the growth status of suckling piglets; however, the difference in the metabolic status between different treatment groups still remains unclear.
32779675	7	35	theme	least-squares	1174:1186	arg1	analysis					1201:1208	partial least-squares discriminant analysis	1166:1208	partial least-squares discriminant analysis	1166:1208	Principal component analysis and partial least-squares discriminant analysis showed a dramatic difference in the composition of hepatic metabolic profiles between the two groups.
32779675	2	36	theme	metabolic	459:467	arg1	status					469:474	the metabolic status	455:474	the metabolic status	455:474	Our previous study found that an early-life galactooligosaccharide (GOS) intervention could improve the growth status of suckling piglets; however, the difference in the metabolic status between different treatment groups still remains unclear.
32779675	10	37	theme	factor	1766:1771	arg1	phosphorylation					1646:1660	the phosphorylation	1642:1660	the phosphorylation of AMP-activated protein kinase	1642:1692	Additionally, with the accumulation of antioxidant-related metabolites in the GOS piglet liver, the hepatic mitochondrial antioxidant capacity increased, and the phosphorylation of AMP-activated protein kinase and the protein expression levels of nuclear factor-erythroid-2-related factor 2 and heme oxygenase-1 were also upregulated in the liver.
32779675	10	37	theme	factor	1766:1771	arg1	levels					1721:1726	the protein expression levels	1698:1726	the protein expression levels of nuclear factor-erythroid-2-related factor 2 and heme oxygenase-1	1698:1794	Additionally, with the accumulation of antioxidant-related metabolites in the GOS piglet liver, the hepatic mitochondrial antioxidant capacity increased, and the phosphorylation of AMP-activated protein kinase and the protein expression levels of nuclear factor-erythroid-2-related factor 2 and heme oxygenase-1 were also upregulated in the liver.
32779675	2	38	theme	GOS	357:359	arg1	intervention					362:373	an early-life galactooligosaccharide (GOS) intervention	319:373	an early-life galactooligosaccharide (GOS) intervention	319:373	Our previous study found that an early-life galactooligosaccharide (GOS) intervention could improve the growth status of suckling piglets; however, the difference in the metabolic status between different treatment groups still remains unclear.
32779675	10	39	theme	factor-erythroid-2-related	1739:1764	arg1	factor					1766:1771	nuclear factor-erythroid-2-related factor 2	1731:1773	nuclear factor-erythroid-2-related factor 2	1731:1773	Additionally, with the accumulation of antioxidant-related metabolites in the GOS piglet liver, the hepatic mitochondrial antioxidant capacity increased, and the phosphorylation of AMP-activated protein kinase and the protein expression levels of nuclear factor-erythroid-2-related factor 2 and heme oxygenase-1 were also upregulated in the liver.
32779675	6	40	theme	lower	1014:1018	arg1	concentration					1044:1056	a lower total serum cholesterol concentration	1012:1056	a lower total serum cholesterol concentration on day 8	1012:1065	Compared with the CON piglets, the GOS piglets showed a lower total serum cholesterol concentration on day 8, but a higher concentration of serum HDL-C and albumin on day 21.
32779675	3	41	theme	piglets	684:690	arg1	profiles					672:679	the hepatic metabolic profiles	650:679	the hepatic metabolic profiles of piglets	650:690	In the present study, sixty neonatal piglets were used to investigate the effects of early-life GOS intervention on the hepatic metabolic profiles of piglets.
32779675	8	42	theme	GOS	1316:1318	arg1	intervention					1320:1331	The GOS intervention	1312:1331	The GOS intervention	1312:1331	The GOS intervention mainly affected the lipid and amino acid metabolism of the piglets.
32779675	2	43	theme	galactooligosaccharide	333:354	arg1	intervention					362:373	an early-life galactooligosaccharide (GOS) intervention	319:373	an early-life galactooligosaccharide (GOS) intervention	319:373	Our previous study found that an early-life galactooligosaccharide (GOS) intervention could improve the growth status of suckling piglets; however, the difference in the metabolic status between different treatment groups still remains unclear.
32779675	11	44	theme	new	1868:1870	arg1	insights					1872:1879	new insights	1868:1879	new insights into the effects of galactooligosaccharides on the growth of suckling piglets	1868:1957	In conclusion, our findings provide new insights into the effects of galactooligosaccharides on the growth of suckling piglets.
32779675	7	45	theme	component	1143:1151	arg1	analysis					1153:1160	Principal component analysis	1133:1160	Principal component analysis	1133:1160	Principal component analysis and partial least-squares discriminant analysis showed a dramatic difference in the composition of hepatic metabolic profiles between the two groups.
32779675	10	46	theme	oxygenase-1	1784:1794	arg1	phosphorylation					1646:1660	the phosphorylation	1642:1660	the phosphorylation of AMP-activated protein kinase	1642:1692	Additionally, with the accumulation of antioxidant-related metabolites in the GOS piglet liver, the hepatic mitochondrial antioxidant capacity increased, and the phosphorylation of AMP-activated protein kinase and the protein expression levels of nuclear factor-erythroid-2-related factor 2 and heme oxygenase-1 were also upregulated in the liver.
32779675	10	46	theme	oxygenase-1	1784:1794	arg1	levels					1721:1726	the protein expression levels	1698:1726	the protein expression levels of nuclear factor-erythroid-2-related factor 2 and heme oxygenase-1	1698:1794	Additionally, with the accumulation of antioxidant-related metabolites in the GOS piglet liver, the hepatic mitochondrial antioxidant capacity increased, and the phosphorylation of AMP-activated protein kinase and the protein expression levels of nuclear factor-erythroid-2-related factor 2 and heme oxygenase-1 were also upregulated in the liver.
32779675	6	47	theme	GOS	993:995	arg1	piglets					997:1003	the GOS piglets	989:1003	the GOS piglets	989:1003	Compared with the CON piglets, the GOS piglets showed a lower total serum cholesterol concentration on day 8, but a higher concentration of serum HDL-C and albumin on day 21.
32779675	10	48	theme	nuclear	1731:1737	arg1	factor					1766:1771	nuclear factor-erythroid-2-related factor 2	1731:1773	nuclear factor-erythroid-2-related factor 2	1731:1773	Additionally, with the accumulation of antioxidant-related metabolites in the GOS piglet liver, the hepatic mitochondrial antioxidant capacity increased, and the phosphorylation of AMP-activated protein kinase and the protein expression levels of nuclear factor-erythroid-2-related factor 2 and heme oxygenase-1 were also upregulated in the liver.
32779675	10	49	theme	antioxidant	1606:1616	arg1	capacity					1618:1625	the hepatic mitochondrial antioxidant capacity	1580:1625	the hepatic mitochondrial antioxidant capacity	1580:1625	Additionally, with the accumulation of antioxidant-related metabolites in the GOS piglet liver, the hepatic mitochondrial antioxidant capacity increased, and the phosphorylation of AMP-activated protein kinase and the protein expression levels of nuclear factor-erythroid-2-related factor 2 and heme oxygenase-1 were also upregulated in the liver.
32779675	2	50	theme	previous	293:300	arg1	study					302:306	Our previous study	289:306	Our previous study	289:306	Our previous study found that an early-life galactooligosaccharide (GOS) intervention could improve the growth status of suckling piglets; however, the difference in the metabolic status between different treatment groups still remains unclear.
32779675	11	51	theme	piglets	1951:1957	arg1	growth					1932:1937	the growth	1928:1937	the growth of suckling piglets	1928:1957	In conclusion, our findings provide new insights into the effects of galactooligosaccharides on the growth of suckling piglets.
32779675	10	52	theme	hepatic	1584:1590	arg1	capacity					1618:1625	the hepatic mitochondrial antioxidant capacity	1580:1625	the hepatic mitochondrial antioxidant capacity	1580:1625	Additionally, with the accumulation of antioxidant-related metabolites in the GOS piglet liver, the hepatic mitochondrial antioxidant capacity increased, and the phosphorylation of AMP-activated protein kinase and the protein expression levels of nuclear factor-erythroid-2-related factor 2 and heme oxygenase-1 were also upregulated in the liver.
32779675	11	53	theme	galactooligosaccharides	1901:1923	arg1	effects					1890:1896	the effects	1886:1896	the effects of galactooligosaccharides on the growth of suckling piglets	1886:1957	In conclusion, our findings provide new insights into the effects of galactooligosaccharides on the growth of suckling piglets.
32779675	11	54	from	effects	1890:1896	arg1	growth					1932:1937	the growth	1928:1937	the growth of suckling piglets	1928:1957	In conclusion, our findings provide new insights into the effects of galactooligosaccharides on the growth of suckling piglets.
32779675	10	55	theme	kinase	1687:1692	arg1	phosphorylation					1646:1660	the phosphorylation	1642:1660	the phosphorylation of AMP-activated protein kinase	1642:1692	Additionally, with the accumulation of antioxidant-related metabolites in the GOS piglet liver, the hepatic mitochondrial antioxidant capacity increased, and the phosphorylation of AMP-activated protein kinase and the protein expression levels of nuclear factor-erythroid-2-related factor 2 and heme oxygenase-1 were also upregulated in the liver.
32779675	10	55	theme	kinase	1687:1692	arg1	levels					1721:1726	the protein expression levels	1698:1726	the protein expression levels of nuclear factor-erythroid-2-related factor 2 and heme oxygenase-1	1698:1794	Additionally, with the accumulation of antioxidant-related metabolites in the GOS piglet liver, the hepatic mitochondrial antioxidant capacity increased, and the phosphorylation of AMP-activated protein kinase and the protein expression levels of nuclear factor-erythroid-2-related factor 2 and heme oxygenase-1 were also upregulated in the liver.
32779675	1	56	theme	suckling	198:205	arg1	time					234:237	a critical window time	216:237	a critical window time for the development of human and animal neonates	216:286	The suckling stage is a critical window time for the development of human and animal neonates.
32779675	1	56	theme	suckling	198:205	arg1	stage					207:211	The suckling stage	194:211	The suckling stage	194:211	The suckling stage is a critical window time for the development of human and animal neonates.
32779675	0	57	theme	AMPK/Nrf2	140:148	arg1	pathway					160:166	the AMPK/Nrf2 signaling pathway	136:166	the AMPK/Nrf2 signaling pathway in suckling piglet liver	136:191	Early galactooligosaccharide intervention alters the metabolic profile, improves the antioxidant capacity of mitochondria and activates the AMPK/Nrf2 signaling pathway in suckling piglet liver.
32779675	10	58	theme	AMP-activated	1665:1677	arg1	kinase					1687:1692	AMP-activated protein kinase	1665:1692	AMP-activated protein kinase	1665:1692	Additionally, with the accumulation of antioxidant-related metabolites in the GOS piglet liver, the hepatic mitochondrial antioxidant capacity increased, and the phosphorylation of AMP-activated protein kinase and the protein expression levels of nuclear factor-erythroid-2-related factor 2 and heme oxygenase-1 were also upregulated in the liver.
32779675	4	59	dep	solution	790:797	arg1	mL					803:804	10 mL	800:804	10 mL for 1 g per kg body weight per day	800:839	In the first week after birth, the piglets in the GOS and control (CON) groups were fed with GOS solution (10 mL for 1 g per kg body weight per day) and physiological saline, respectively.
32779675	6	60	theme	serum	1026:1030	arg1	concentration					1044:1056	a lower total serum cholesterol concentration	1012:1056	a lower total serum cholesterol concentration on day 8	1012:1065	Compared with the CON piglets, the GOS piglets showed a lower total serum cholesterol concentration on day 8, but a higher concentration of serum HDL-C and albumin on day 21.
32779675	10	61	theme	expression	1710:1719	arg1	levels					1721:1726	the protein expression levels	1698:1726	the protein expression levels of nuclear factor-erythroid-2-related factor 2 and heme oxygenase-1	1698:1794	Additionally, with the accumulation of antioxidant-related metabolites in the GOS piglet liver, the hepatic mitochondrial antioxidant capacity increased, and the phosphorylation of AMP-activated protein kinase and the protein expression levels of nuclear factor-erythroid-2-related factor 2 and heme oxygenase-1 were also upregulated in the liver.
32779675	5	62	from	group	922:926	arg1	piglets					904:910	six piglets	900:910	six piglets from each group	900:926	On days 8 and 21, six piglets from each group were euthanized for sampling.
32779675	1	63	theme	critical	218:225	arg1	time					234:237	a critical window time	216:237	a critical window time for the development of human and animal neonates	216:286	The suckling stage is a critical window time for the development of human and animal neonates.
32779675	1	63	theme	critical	218:225	arg1	stage					207:211	The suckling stage	194:211	The suckling stage	194:211	The suckling stage is a critical window time for the development of human and animal neonates.
32779675	4	64	theme	physiological	846:858	arg1	saline					860:865	physiological saline	846:865	physiological saline	846:865	In the first week after birth, the piglets in the GOS and control (CON) groups were fed with GOS solution (10 mL for 1 g per kg body weight per day) and physiological saline, respectively.
32779675	0	65	theme	suckling	171:178	arg1	liver					187:191	suckling piglet liver	171:191	suckling piglet liver	171:191	Early galactooligosaccharide intervention alters the metabolic profile, improves the antioxidant capacity of mitochondria and activates the AMPK/Nrf2 signaling pathway in suckling piglet liver.
32779675	4	66	theme	body	821:824	arg1	weight					826:831	kg body weight	818:831	kg body weight per day	818:839	In the first week after birth, the piglets in the GOS and control (CON) groups were fed with GOS solution (10 mL for 1 g per kg body weight per day) and physiological saline, respectively.
32779675	8	67	theme	acid	1369:1372	arg1	metabolism					1374:1383	the lipid and amino acid metabolism	1349:1383	metabolism	1374:1383	The GOS intervention mainly affected the lipid and amino acid metabolism of the piglets.
32779675	6	68	theme	serum	1098:1102	arg1	concentration					1081:1093	a higher concentration	1072:1093	a higher concentration of serum HDL-C and albumin	1072:1120	Compared with the CON piglets, the GOS piglets showed a lower total serum cholesterol concentration on day 8, but a higher concentration of serum HDL-C and albumin on day 21.
32779675	3	69	used	used	584:587	arg2	piglets					571:577	sixty neonatal piglets	556:577	sixty neonatal piglets	556:577	In the present study, sixty neonatal piglets were used to investigate the effects of early-life GOS intervention on the hepatic metabolic profiles of piglets.
32779675	3	70	theme	early-life	619:628	arg1	intervention					634:645	early-life GOS intervention	619:645	early-life GOS intervention	619:645	In the present study, sixty neonatal piglets were used to investigate the effects of early-life GOS intervention on the hepatic metabolic profiles of piglets.
32779675	8	71	theme	piglets	1392:1398	arg1	lipid					1353:1357	the lipid and amino acid metabolism	1349:1383	lipid	1353:1357	The GOS intervention mainly affected the lipid and amino acid metabolism of the piglets.
32779675	8	71	theme	piglets	1392:1398	arg1	metabolism					1374:1383	the lipid and amino acid metabolism	1349:1383	metabolism	1374:1383	The GOS intervention mainly affected the lipid and amino acid metabolism of the piglets.
32779675	6	72	dep	serum	1098:1102	arg1	HDL-C					1104:1108	HDL-C	1104:1108	HDL-C	1104:1108	Compared with the CON piglets, the GOS piglets showed a lower total serum cholesterol concentration on day 8, but a higher concentration of serum HDL-C and albumin on day 21.
32779675	9	73	theme	mRNA	1416:1419	arg1	levels					1421:1426	the mRNA levels	1412:1426	the mRNA levels of CPT-1	1412:1435	Meanwhile, the mRNA levels of CPT-1 and PCG1α increased in the GOS piglets' liver.
32779675	2	74	theme	different	484:492	arg1	groups					504:509	different treatment groups	484:509	different treatment groups	484:509	Our previous study found that an early-life galactooligosaccharide (GOS) intervention could improve the growth status of suckling piglets; however, the difference in the metabolic status between different treatment groups still remains unclear.
32779675	0	75	theme	mitochondria	109:120	arg1	capacity					97:104	the antioxidant capacity	81:104	the antioxidant capacity of mitochondria	81:120	Early galactooligosaccharide intervention alters the metabolic profile, improves the antioxidant capacity of mitochondria and activates the AMPK/Nrf2 signaling pathway in suckling piglet liver.
32779675	11	76	theme	suckling	1942:1949	arg1	piglets					1951:1957	suckling piglets	1942:1957	suckling piglets	1942:1957	In conclusion, our findings provide new insights into the effects of galactooligosaccharides on the growth of suckling piglets.
32779675	4	77	theme	GOS	743:745	arg1	groups					765:770	the GOS and control (CON) groups	739:770	the GOS and control (CON) groups	739:770	In the first week after birth, the piglets in the GOS and control (CON) groups were fed with GOS solution (10 mL for 1 g per kg body weight per day) and physiological saline, respectively.
32779675	3	78	from	effects	608:614	arg1	profiles					672:679	the hepatic metabolic profiles	650:679	the hepatic metabolic profiles of piglets	650:690	In the present study, sixty neonatal piglets were used to investigate the effects of early-life GOS intervention on the hepatic metabolic profiles of piglets.
32779675	7	79	theme	dramatic	1219:1226	arg1	difference					1228:1237	a dramatic difference	1217:1237	a dramatic difference in the composition of hepatic metabolic profiles between the two groups	1217:1309	Principal component analysis and partial least-squares discriminant analysis showed a dramatic difference in the composition of hepatic metabolic profiles between the two groups.
32779675	4	80	theme	control	751:757	arg1	groups					765:770	the GOS and control (CON) groups	739:770	the GOS and control (CON) groups	739:770	In the first week after birth, the piglets in the GOS and control (CON) groups were fed with GOS solution (10 mL for 1 g per kg body weight per day) and physiological saline, respectively.
32779675	10	81	theme	antioxidant-related	1523:1541	arg1	metabolites					1543:1553	antioxidant-related metabolites	1523:1553	antioxidant-related metabolites	1523:1553	Additionally, with the accumulation of antioxidant-related metabolites in the GOS piglet liver, the hepatic mitochondrial antioxidant capacity increased, and the phosphorylation of AMP-activated protein kinase and the protein expression levels of nuclear factor-erythroid-2-related factor 2 and heme oxygenase-1 were also upregulated in the liver.
32779675	0	82	theme	galactooligosaccharide	6:27	arg1	intervention					29:40	Early galactooligosaccharide intervention	0:40	Early galactooligosaccharide intervention	0:40	Early galactooligosaccharide intervention alters the metabolic profile, improves the antioxidant capacity of mitochondria and activates the AMPK/Nrf2 signaling pathway in suckling piglet liver.
32779675	6	83	theme	albumin	1114:1120	arg1	concentration					1081:1093	a higher concentration	1072:1093	a higher concentration of serum HDL-C and albumin	1072:1120	Compared with the CON piglets, the GOS piglets showed a lower total serum cholesterol concentration on day 8, but a higher concentration of serum HDL-C and albumin on day 21.
32779675	7	84	theme	discriminant	1188:1199	arg1	analysis					1201:1208	partial least-squares discriminant analysis	1166:1208	partial least-squares discriminant analysis	1166:1208	Principal component analysis and partial least-squares discriminant analysis showed a dramatic difference in the composition of hepatic metabolic profiles between the two groups.
32779675	10	85	theme	heme	1779:1782	arg1	oxygenase-1					1784:1794	heme oxygenase-1	1779:1794	heme oxygenase-1	1779:1794	Additionally, with the accumulation of antioxidant-related metabolites in the GOS piglet liver, the hepatic mitochondrial antioxidant capacity increased, and the phosphorylation of AMP-activated protein kinase and the protein expression levels of nuclear factor-erythroid-2-related factor 2 and heme oxygenase-1 were also upregulated in the liver.
32779675	10	86	theme	GOS	1562:1564	arg1	liver					1573:1577	the GOS piglet liver	1558:1577	the GOS piglet liver	1558:1577	Additionally, with the accumulation of antioxidant-related metabolites in the GOS piglet liver, the hepatic mitochondrial antioxidant capacity increased, and the phosphorylation of AMP-activated protein kinase and the protein expression levels of nuclear factor-erythroid-2-related factor 2 and heme oxygenase-1 were also upregulated in the liver.
32779675	0	87	theme	metabolic	53:61	arg1	profile					63:69	the metabolic profile	49:69	the metabolic profile	49:69	Early galactooligosaccharide intervention alters the metabolic profile, improves the antioxidant capacity of mitochondria and activates the AMPK/Nrf2 signaling pathway in suckling piglet liver.
32779675	7	88	theme	partial	1166:1172	arg1	analysis					1201:1208	partial least-squares discriminant analysis	1166:1208	partial least-squares discriminant analysis	1166:1208	Principal component analysis and partial least-squares discriminant analysis showed a dramatic difference in the composition of hepatic metabolic profiles between the two groups.
32779675	3	89	theme	intervention	634:645	arg1	effects					608:614	the effects	604:614	the effects of early-life GOS intervention on the hepatic metabolic profiles of piglets	604:690	In the present study, sixty neonatal piglets were used to investigate the effects of early-life GOS intervention on the hepatic metabolic profiles of piglets.
32779675	2	90	theme	suckling	410:417	arg1	piglets					419:425	suckling piglets	410:425	suckling piglets	410:425	Our previous study found that an early-life galactooligosaccharide (GOS) intervention could improve the growth status of suckling piglets; however, the difference in the metabolic status between different treatment groups still remains unclear.
32779675	10	91	from	accumulation	1507:1518	arg1	liver					1573:1577	the GOS piglet liver	1558:1577	the GOS piglet liver	1558:1577	Additionally, with the accumulation of antioxidant-related metabolites in the GOS piglet liver, the hepatic mitochondrial antioxidant capacity increased, and the phosphorylation of AMP-activated protein kinase and the protein expression levels of nuclear factor-erythroid-2-related factor 2 and heme oxygenase-1 were also upregulated in the liver.
32779675	6	92	theme	total	1020:1024	arg1	concentration					1044:1056	a lower total serum cholesterol concentration	1012:1056	a lower total serum cholesterol concentration on day 8	1012:1065	Compared with the CON piglets, the GOS piglets showed a lower total serum cholesterol concentration on day 8, but a higher concentration of serum HDL-C and albumin on day 21.
32779675	4	93	theme	GOS	786:788	arg1	solution					790:797	GOS solution	786:797	GOS solution (10 mL for 1 g per kg body weight per day)	786:840	In the first week after birth, the piglets in the GOS and control (CON) groups were fed with GOS solution (10 mL for 1 g per kg body weight per day) and physiological saline, respectively.
32779675	7	94	theme	Principal	1133:1141	arg1	analysis					1153:1160	Principal component analysis	1133:1160	Principal component analysis	1133:1160	Principal component analysis and partial least-squares discriminant analysis showed a dramatic difference in the composition of hepatic metabolic profiles between the two groups.
32779675	0	95	from	pathway	160:166	arg1	liver					187:191	suckling piglet liver	171:191	suckling piglet liver	171:191	Early galactooligosaccharide intervention alters the metabolic profile, improves the antioxidant capacity of mitochondria and activates the AMPK/Nrf2 signaling pathway in suckling piglet liver.
32779675	3	96	theme	metabolic	662:670	arg1	profiles					672:679	the hepatic metabolic profiles	650:679	the hepatic metabolic profiles of piglets	650:690	In the present study, sixty neonatal piglets were used to investigate the effects of early-life GOS intervention on the hepatic metabolic profiles of piglets.
32779675	2	97	theme	early-life	322:331	arg1	intervention					362:373	an early-life galactooligosaccharide (GOS) intervention	319:373	an early-life galactooligosaccharide (GOS) intervention	319:373	Our previous study found that an early-life galactooligosaccharide (GOS) intervention could improve the growth status of suckling piglets; however, the difference in the metabolic status between different treatment groups still remains unclear.
32375988	11	0	theme	wall	1117:1120	arg1	components					1094:1103	Amino acid components	1083:1103	Amino acid components of the cell wall	1083:1120	Amino acid components of the cell wall were l-alanine, d-glucose, l-lysine, glycine and aspartic acid.
32375988	5	1	theme	gene	500:503	arg1	sequences					505:513	The 16S rRNA gene sequences	487:513	The 16S rRNA gene sequences of the strain	487:527	The 16S rRNA gene sequences of the strain showed 97.06-97.34, 96.17-96.31 and 95.93-96.07 % similarity to the type strains of Jeotgalibaca arthritidis, J. porci and J. dankookensis, respectively.
32375988	13	2	theme	Average	1235:1241	arg1	identity					1254:1261	Average nucleotide identity	1235:1261	Average nucleotide identity between strain H21T32T and J. arthritidis CECT 9157T	1235:1314	Average nucleotide identity between strain H21T32T and J. arthritidis CECT 9157T was 77.02 %, confirming that strain H21T32T represents a novel species of the genus Jeotgalibaca, for which the name Jeotgalibaca ciconiae sp.
32375988	6	3	dep	10-37 °C	702:709	arg1	temperature					720:730	optimum temperature	712:730	optimum temperature: 30 °C	712:737	The strain grew at 10-37 °C (optimum temperature: 30 °C), with 0-7 % (w/v) NaCl (optimum salinity: 0.5 %) and at pH 7-9 (optimum pH: 8).
32375988	6	4	theme	optimum	804:810	arg1	pH					812:813	optimum pH	804:813	optimum pH: 8	804:816	The strain grew at 10-37 °C (optimum temperature: 30 °C), with 0-7 % (w/v) NaCl (optimum salinity: 0.5 %) and at pH 7-9 (optimum pH: 8).
32375988	12	5	theme	DNA	1190:1192	arg1	content					1198:1204	The DNA G+C content	1186:1204	The DNA G+C content of the strain	1186:1218	The DNA G+C content of the strain was 37.1 mol%.
32375988	12	5	theme	DNA	1190:1192	arg1	%					1232:1232	37.1 mol%	1224:1232	37.1 mol%	1224:1232	The DNA G+C content of the strain was 37.1 mol%.
32375988	7	6	theme	main	824:827	arg1	C16 					855:858	C16 	855:858	C16 	855:858	The main cellular fatty acids were C16 : 1 ω9c, C18 : 1 ω9c and C16 : 0.
32375988	7	6	theme	main	824:827	arg1	acids					844:848	The main cellular fatty acids	820:848	The main cellular fatty acids	820:848	The main cellular fatty acids were C16 : 1 ω9c, C18 : 1 ω9c and C16 : 0.
32375988	6	7	theme	optimum	764:770	arg1	salinity					772:779	optimum salinity	764:779	optimum salinity: 0.5 %	764:786	The strain grew at 10-37 °C (optimum temperature: 30 °C), with 0-7 % (w/v) NaCl (optimum salinity: 0.5 %) and at pH 7-9 (optimum pH: 8).
32375988	6	8	dep	temperature	720:730	arg1	30 °C					733:737	30 °C	733:737	optimum temperature: 30 °C	712:737	The strain grew at 10-37 °C (optimum temperature: 30 °C), with 0-7 % (w/v) NaCl (optimum salinity: 0.5 %) and at pH 7-9 (optimum pH: 8).
32375988	6	9	dep	pH	796:797	arg1	pH					812:813	optimum pH	804:813	optimum pH: 8	804:816	The strain grew at 10-37 °C (optimum temperature: 30 °C), with 0-7 % (w/v) NaCl (optimum salinity: 0.5 %) and at pH 7-9 (optimum pH: 8).
32375988	13	10	dep	Jeotgalibaca	1433:1444	arg1	ciconiae					1446:1453	ciconiae	1446:1453	ciconiae	1446:1453	Average nucleotide identity between strain H21T32T and J. arthritidis CECT 9157T was 77.02 %, confirming that strain H21T32T represents a novel species of the genus Jeotgalibaca, for which the name Jeotgalibaca ciconiae sp.
32375988	2	11	theme	novel	81:85	arg1	bacterium					152:160	A novel, Gram-stain-positive, non-spore-forming, facultatively anaerobic bacterium	79:160	A novel, Gram-stain-positive, non-spore-forming, facultatively anaerobic bacterium	79:160	A novel, Gram-stain-positive, non-spore-forming, facultatively anaerobic bacterium, designated strain H21T32T, was isolated from the faeces of an Oriental stork, Ciconia boyciana.
32375988	7	12	theme	cellular	829:836	arg1	C16 					855:858	C16 	855:858	C16 	855:858	The main cellular fatty acids were C16 : 1 ω9c, C18 : 1 ω9c and C16 : 0.
32375988	7	12	theme	cellular	829:836	arg1	acids					844:848	The main cellular fatty acids	820:848	The main cellular fatty acids	820:848	The main cellular fatty acids were C16 : 1 ω9c, C18 : 1 ω9c and C16 : 0.
32375988	6	13	theme	%	750:750	arg1	NaCl					758:761	0-7 % (w/v) NaCl	746:761	0-7 % (w/v) NaCl (optimum salinity: 0.5 %)	746:787	The strain grew at 10-37 °C (optimum temperature: 30 °C), with 0-7 % (w/v) NaCl (optimum salinity: 0.5 %) and at pH 7-9 (optimum pH: 8).
32375988	12	14	theme	strain	1213:1218	arg1	content					1198:1204	The DNA G+C content	1186:1204	The DNA G+C content of the strain	1186:1218	The DNA G+C content of the strain was 37.1 mol%.
32375988	12	14	theme	strain	1213:1218	arg1	%					1232:1232	37.1 mol%	1224:1232	37.1 mol%	1224:1232	The DNA G+C content of the strain was 37.1 mol%.
32375988	13	15	theme	genus	1394:1398	arg1	Jeotgalibaca					1400:1411	the genus Jeotgalibaca	1390:1411	the genus Jeotgalibaca	1390:1411	Average nucleotide identity between strain H21T32T and J. arthritidis CECT 9157T was 77.02 %, confirming that strain H21T32T represents a novel species of the genus Jeotgalibaca, for which the name Jeotgalibaca ciconiae sp.
32375988	12	16	theme	G+C	1194:1196	arg1	content					1198:1204	The DNA G+C content	1186:1204	The DNA G+C content of the strain	1186:1218	The DNA G+C content of the strain was 37.1 mol%.
32375988	12	16	theme	G+C	1194:1196	arg1	%					1232:1232	37.1 mol%	1224:1232	37.1 mol%	1224:1232	The DNA G+C content of the strain was 37.1 mol%.
32375988	11	17	theme	cell	1112:1115	arg1	wall					1117:1120	the cell wall	1108:1120	the cell wall	1108:1120	Amino acid components of the cell wall were l-alanine, d-glucose, l-lysine, glycine and aspartic acid.
32375988	4	18	theme	Strain	369:374	arg1	H21T32T					376:382	Strain H21T32T	369:382	Strain H21T32T	369:382	Strain H21T32T belonged to the genus Jeotgalibaca, family Carnobacteriaceae, order Lactobacillales and class Bacilli.
32375988	10	19	theme	peptidoglycan	1034:1046	arg1	components					1016:1025	Sugar components	1010:1025	Sugar components of the peptidoglycan	1010:1046	Sugar components of the peptidoglycan were rhamnose, ribose and glucose.
32375988	12	20	theme	37.1 mol	1224:1231	arg1	content					1198:1204	The DNA G+C content	1186:1204	The DNA G+C content of the strain	1186:1218	The DNA G+C content of the strain was 37.1 mol%.
32375988	12	20	theme	37.1 mol	1224:1231	arg1	%					1232:1232	37.1 mol%	1224:1232	37.1 mol%	1224:1232	The DNA G+C content of the strain was 37.1 mol%.
32375988	13	21	theme	Jeotgalibaca	1433:1444	arg1	sp					1455:1456	the name Jeotgalibaca ciconiae sp	1424:1456	the name Jeotgalibaca ciconiae sp	1424:1456	Average nucleotide identity between strain H21T32T and J. arthritidis CECT 9157T was 77.02 %, confirming that strain H21T32T represents a novel species of the genus Jeotgalibaca, for which the name Jeotgalibaca ciconiae sp.
32375988	5	22	dep	porci	642:646	arg1	J.					639:640	J.	639:640	J.	639:640	The 16S rRNA gene sequences of the strain showed 97.06-97.34, 96.17-96.31 and 95.93-96.07 % similarity to the type strains of Jeotgalibaca arthritidis, J. porci and J. dankookensis, respectively.
32375988	2	23	attach	isolated	194:201	arg1	faeces					212:217	the faeces	208:217	the faeces of an Oriental stork	208:238	A novel, Gram-stain-positive, non-spore-forming, facultatively anaerobic bacterium, designated strain H21T32T, was isolated from the faeces of an Oriental stork, Ciconia boyciana.
32375988	2	23	attach	isolated	194:201	arg2	bacterium					152:160	A novel, Gram-stain-positive, non-spore-forming, facultatively anaerobic bacterium	79:160	A novel, Gram-stain-positive, non-spore-forming, facultatively anaerobic bacterium	79:160	A novel, Gram-stain-positive, non-spore-forming, facultatively anaerobic bacterium, designated strain H21T32T, was isolated from the faeces of an Oriental stork, Ciconia boyciana.
32375988	0	24	theme	ciconiae	13:20	arg1	sp					22:23	Jeotgalibaca ciconiae sp	0:23	Jeotgalibaca ciconiae sp.	0:24	Jeotgalibaca ciconiae sp.
32375988	4	25	theme	order	446:450	arg1	Lactobacillales					452:466	order Lactobacillales	446:466	order Lactobacillales	446:466	Strain H21T32T belonged to the genus Jeotgalibaca, family Carnobacteriaceae, order Lactobacillales and class Bacilli.
32375988	5	26	theme	type	597:600	arg1	strains					602:608	the type strains	593:608	the type strains of Jeotgalibaca arthritidis, J. porci and J. dankookensis	593:666	The 16S rRNA gene sequences of the strain showed 97.06-97.34, 96.17-96.31 and 95.93-96.07 % similarity to the type strains of Jeotgalibaca arthritidis, J. porci and J. dankookensis, respectively.
32375988	13	27	theme	Jeotgalibaca	1400:1411	arg1	species					1379:1385	a novel species	1371:1385	a novel species	1371:1385	Average nucleotide identity between strain H21T32T and J. arthritidis CECT 9157T was 77.02 %, confirming that strain H21T32T represents a novel species of the genus Jeotgalibaca, for which the name Jeotgalibaca ciconiae sp.
32375988	1	28	theme	Oriental	63:70	arg1	stork					72:76	an Oriental stork	60:76	an Oriental stork	60:76	nov., isolated from the faeces of an Oriental stork.
32375988	13	29	theme	name	1428:1431	arg1	sp					1455:1456	the name Jeotgalibaca ciconiae sp	1424:1456	the name Jeotgalibaca ciconiae sp	1424:1456	Average nucleotide identity between strain H21T32T and J. arthritidis CECT 9157T was 77.02 %, confirming that strain H21T32T represents a novel species of the genus Jeotgalibaca, for which the name Jeotgalibaca ciconiae sp.
32375988	0	30	theme	Jeotgalibaca	0:11	arg1	sp					22:23	Jeotgalibaca ciconiae sp	0:23	Jeotgalibaca ciconiae sp.	0:24	Jeotgalibaca ciconiae sp.
32375988	1	31	theme	stork	72:76	arg1	faeces					50:55	the faeces	46:55	the faeces of an Oriental stork	46:76	nov., isolated from the faeces of an Oriental stork.
32375988	6	32	dep	pH	812:813	arg1	8					816:816	8	816:816	8	816:816	The strain grew at 10-37 °C (optimum temperature: 30 °C), with 0-7 % (w/v) NaCl (optimum salinity: 0.5 %) and at pH 7-9 (optimum pH: 8).
32375988	5	33	theme	dankookensis	655:666	arg1	strains					602:608	the type strains	593:608	the type strains of Jeotgalibaca arthritidis, J. porci and J. dankookensis	593:666	The 16S rRNA gene sequences of the strain showed 97.06-97.34, 96.17-96.31 and 95.93-96.07 % similarity to the type strains of Jeotgalibaca arthritidis, J. porci and J. dankookensis, respectively.
32375988	3	34	theme	solid	338:342	arg1	medium					344:349	solid medium	338:349	solid medium	338:349	Cells formed cocci grouped in pairs, tetrads or conglomerates, and colonies on solid medium were pale yellow.
32375988	15	35	theme	=KCTC	1505:1509	arg1	33222T					1522:1527	=KCTC 33991T=JCM 33222T	1505:1527	=KCTC 33991T=JCM 33222T	1505:1527	The type strain is H21T32T (=KCTC 33991T=JCM 33222T).
32375988	15	35	theme	=KCTC	1505:1509	arg1	H21T32T					1496:1502	H21T32T	1496:1502	H21T32T (=KCTC 33991T=JCM 33222T)	1496:1528	The type strain is H21T32T (=KCTC 33991T=JCM 33222T).
32375988	7	36	theme	fatty	838:842	arg1	C16 					855:858	C16 	855:858	C16 	855:858	The main cellular fatty acids were C16 : 1 ω9c, C18 : 1 ω9c and C16 : 0.
32375988	7	36	theme	fatty	838:842	arg1	acids					844:848	The main cellular fatty acids	820:848	The main cellular fatty acids	820:848	The main cellular fatty acids were C16 : 1 ω9c, C18 : 1 ω9c and C16 : 0.
32375988	2	37	theme	strain	174:179	arg1	H21T32T					181:187	strain H21T32T	174:187	strain H21T32T	174:187	A novel, Gram-stain-positive, non-spore-forming, facultatively anaerobic bacterium, designated strain H21T32T, was isolated from the faeces of an Oriental stork, Ciconia boyciana.
32375988	13	38	theme	nucleotide	1243:1252	arg1	identity					1254:1261	Average nucleotide identity	1235:1261	Average nucleotide identity between strain H21T32T and J. arthritidis CECT 9157T	1235:1314	Average nucleotide identity between strain H21T32T and J. arthritidis CECT 9157T was 77.02 %, confirming that strain H21T32T represents a novel species of the genus Jeotgalibaca, for which the name Jeotgalibaca ciconiae sp.
32375988	15	39	theme	33991T=JCM	1511:1520	arg1	33222T					1522:1527	=KCTC 33991T=JCM 33222T	1505:1527	=KCTC 33991T=JCM 33222T	1505:1527	The type strain is H21T32T (=KCTC 33991T=JCM 33222T).
32375988	15	39	theme	33991T=JCM	1511:1520	arg1	H21T32T					1496:1502	H21T32T	1496:1502	H21T32T (=KCTC 33991T=JCM 33222T)	1496:1528	The type strain is H21T32T (=KCTC 33991T=JCM 33222T).
32375988	13	40	theme	J.	1290:1291	arg1	9157T					1310:1314	strain H21T32T and J. arthritidis CECT 9157T	1271:1314	strain H21T32T and J. arthritidis CECT 9157T	1271:1314	Average nucleotide identity between strain H21T32T and J. arthritidis CECT 9157T was 77.02 %, confirming that strain H21T32T represents a novel species of the genus Jeotgalibaca, for which the name Jeotgalibaca ciconiae sp.
32375988	6	41	dep	NaCl	758:761	arg1	salinity					772:779	optimum salinity	764:779	optimum salinity: 0.5 %	764:786	The strain grew at 10-37 °C (optimum temperature: 30 °C), with 0-7 % (w/v) NaCl (optimum salinity: 0.5 %) and at pH 7-9 (optimum pH: 8).
32375988	8	42	theme	polar	903:907	arg1	diphosphatidylglycerol					921:942	diphosphatidylglycerol	921:942	diphosphatidylglycerol	921:942	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol.
32375988	8	42	theme	polar	903:907	arg1	lipids					909:914	The major polar lipids	893:914	The major polar lipids	893:914	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol.
32375988	4	43	theme	genus	400:404	arg1	Jeotgalibaca					406:417	the genus Jeotgalibaca	396:417	the genus Jeotgalibaca	396:417	Strain H21T32T belonged to the genus Jeotgalibaca, family Carnobacteriaceae, order Lactobacillales and class Bacilli.
32375988	7	44	dep	C16 	855:858	arg1	C16 					884:887	C16 	884:887	C16 	884:887	The main cellular fatty acids were C16 : 1 ω9c, C18 : 1 ω9c and C16 : 0.
32375988	7	44	dep	C16 	855:858	arg1	C18 					868:871	C18 	868:871	C18 	868:871	The main cellular fatty acids were C16 : 1 ω9c, C18 : 1 ω9c and C16 : 0.
32375988	7	44	dep	C16 	855:858	arg1	ω9c					876:878	ω9c	876:878	ω9c	876:878	The main cellular fatty acids were C16 : 1 ω9c, C18 : 1 ω9c and C16 : 0.
32375988	7	44	dep	C16 	855:858	arg1	 0					889:890	 0	889:890	C16 : 1 ω9c, C18 : 1 ω9c and C16 : 0	855:890	The main cellular fatty acids were C16 : 1 ω9c, C18 : 1 ω9c and C16 : 0.
32375988	13	45	theme	arthritidis	1293:1303	arg1	9157T					1310:1314	strain H21T32T and J. arthritidis CECT 9157T	1271:1314	strain H21T32T and J. arthritidis CECT 9157T	1271:1314	Average nucleotide identity between strain H21T32T and J. arthritidis CECT 9157T was 77.02 %, confirming that strain H21T32T represents a novel species of the genus Jeotgalibaca, for which the name Jeotgalibaca ciconiae sp.
32375988	6	46	dep	salinity	772:779	arg1	%					786:786	0.5 %	782:786	optimum salinity: 0.5 %	764:786	The strain grew at 10-37 °C (optimum temperature: 30 °C), with 0-7 % (w/v) NaCl (optimum salinity: 0.5 %) and at pH 7-9 (optimum pH: 8).
32375988	8	47	theme	major	897:901	arg1	diphosphatidylglycerol					921:942	diphosphatidylglycerol	921:942	diphosphatidylglycerol	921:942	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol.
32375988	8	47	theme	major	897:901	arg1	lipids					909:914	The major polar lipids	893:914	The major polar lipids	893:914	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol.
32375988	4	48	theme	family	420:425	arg1	Carnobacteriaceae					427:443	family Carnobacteriaceae	420:443	family Carnobacteriaceae	420:443	Strain H21T32T belonged to the genus Jeotgalibaca, family Carnobacteriaceae, order Lactobacillales and class Bacilli.
32375988	13	49	theme	CECT	1305:1308	arg1	9157T					1310:1314	strain H21T32T and J. arthritidis CECT 9157T	1271:1314	strain H21T32T and J. arthritidis CECT 9157T	1271:1314	Average nucleotide identity between strain H21T32T and J. arthritidis CECT 9157T was 77.02 %, confirming that strain H21T32T represents a novel species of the genus Jeotgalibaca, for which the name Jeotgalibaca ciconiae sp.
32375988	3	50	theme	pale	356:359	arg1	yellow					361:366	pale yellow	356:366	pale yellow	356:366	Cells formed cocci grouped in pairs, tetrads or conglomerates, and colonies on solid medium were pale yellow.
32375988	2	51	dep	novel	81:85	arg1	Gram-stain-positive					88:106	Gram-stain-positive	88:106	Gram-stain-positive	88:106	A novel, Gram-stain-positive, non-spore-forming, facultatively anaerobic bacterium, designated strain H21T32T, was isolated from the faeces of an Oriental stork, Ciconia boyciana.
32375988	2	51	dep	novel	81:85	arg1	non-spore-forming					109:125	non-spore-forming	109:125	non-spore-forming	109:125	A novel, Gram-stain-positive, non-spore-forming, facultatively anaerobic bacterium, designated strain H21T32T, was isolated from the faeces of an Oriental stork, Ciconia boyciana.
32375988	2	51	dep	novel	81:85	arg1	anaerobic					142:150	anaerobic	142:150	anaerobic	142:150	A novel, Gram-stain-positive, non-spore-forming, facultatively anaerobic bacterium, designated strain H21T32T, was isolated from the faeces of an Oriental stork, Ciconia boyciana.
32375988	3	52	from	colonies	326:333	arg1	medium					344:349	solid medium	338:349	solid medium	338:349	Cells formed cocci grouped in pairs, tetrads or conglomerates, and colonies on solid medium were pale yellow.
32375988	6	53	theme	optimum	712:718	arg1	temperature					720:730	optimum temperature	712:730	optimum temperature: 30 °C	712:737	The strain grew at 10-37 °C (optimum temperature: 30 °C), with 0-7 % (w/v) NaCl (optimum salinity: 0.5 %) and at pH 7-9 (optimum pH: 8).
32375988	5	54	theme	porci	642:646	arg1	strains					602:608	the type strains	593:608	the type strains of Jeotgalibaca arthritidis, J. porci and J. dankookensis	593:666	The 16S rRNA gene sequences of the strain showed 97.06-97.34, 96.17-96.31 and 95.93-96.07 % similarity to the type strains of Jeotgalibaca arthritidis, J. porci and J. dankookensis, respectively.
32375988	13	55	theme	novel	1373:1377	arg1	species					1379:1385	a novel species	1371:1385	a novel species	1371:1385	Average nucleotide identity between strain H21T32T and J. arthritidis CECT 9157T was 77.02 %, confirming that strain H21T32T represents a novel species of the genus Jeotgalibaca, for which the name Jeotgalibaca ciconiae sp.
32375988	9	56	theme	Respiratory	970:980	arg1	quinones					982:989	Respiratory quinones	970:989	Respiratory quinones	970:989	Respiratory quinones were not detected.
32375988	5	57	dep	%	577:577	arg1	95.93-96.07 					565:576	95.93-96.07 	565:576	95.93-96.07 	565:576	The 16S rRNA gene sequences of the strain showed 97.06-97.34, 96.17-96.31 and 95.93-96.07 % similarity to the type strains of Jeotgalibaca arthritidis, J. porci and J. dankookensis, respectively.
32375988	5	57	dep	%	577:577	arg1	96.17-96.31					549:559	96.17-96.31	549:559	96.17-96.31	549:559	The 16S rRNA gene sequences of the strain showed 97.06-97.34, 96.17-96.31 and 95.93-96.07 % similarity to the type strains of Jeotgalibaca arthritidis, J. porci and J. dankookensis, respectively.
32375988	11	58	theme	Amino	1083:1087	arg1	components					1094:1103	Amino acid components	1083:1103	Amino acid components of the cell wall	1083:1120	Amino acid components of the cell wall were l-alanine, d-glucose, l-lysine, glycine and aspartic acid.
32375988	5	59	theme	strain	522:527	arg1	sequences					505:513	The 16S rRNA gene sequences	487:513	The 16S rRNA gene sequences of the strain	487:527	The 16S rRNA gene sequences of the strain showed 97.06-97.34, 96.17-96.31 and 95.93-96.07 % similarity to the type strains of Jeotgalibaca arthritidis, J. porci and J. dankookensis, respectively.
32375988	13	60	theme	strain	1271:1276	arg1	9157T					1310:1314	strain H21T32T and J. arthritidis CECT 9157T	1271:1314	strain H21T32T and J. arthritidis CECT 9157T	1271:1314	Average nucleotide identity between strain H21T32T and J. arthritidis CECT 9157T was 77.02 %, confirming that strain H21T32T represents a novel species of the genus Jeotgalibaca, for which the name Jeotgalibaca ciconiae sp.
32375988	5	61	dep	similarity	579:588	arg1	%					577:577	%	577:577	%	577:577	The 16S rRNA gene sequences of the strain showed 97.06-97.34, 96.17-96.31 and 95.93-96.07 % similarity to the type strains of Jeotgalibaca arthritidis, J. porci and J. dankookensis, respectively.
32375988	15	62	theme	type	1481:1484	arg1	H21T32T					1496:1502	H21T32T	1496:1502	H21T32T (=KCTC 33991T=JCM 33222T)	1496:1528	The type strain is H21T32T (=KCTC 33991T=JCM 33222T).
32375988	15	62	theme	type	1481:1484	arg1	strain					1486:1491	The type strain	1477:1491	The type strain	1477:1491	The type strain is H21T32T (=KCTC 33991T=JCM 33222T).
32375988	11	63	theme	acid	1089:1092	arg1	components					1094:1103	Amino acid components	1083:1103	Amino acid components of the cell wall	1083:1120	Amino acid components of the cell wall were l-alanine, d-glucose, l-lysine, glycine and aspartic acid.
32375988	2	64	theme	stork	234:238	arg1	faeces					212:217	the faeces	208:217	the faeces of an Oriental stork	208:238	A novel, Gram-stain-positive, non-spore-forming, facultatively anaerobic bacterium, designated strain H21T32T, was isolated from the faeces of an Oriental stork, Ciconia boyciana.
32375988	13	65	theme	H21T32T	1278:1284	arg1	9157T					1310:1314	strain H21T32T and J. arthritidis CECT 9157T	1271:1314	strain H21T32T and J. arthritidis CECT 9157T	1271:1314	Average nucleotide identity between strain H21T32T and J. arthritidis CECT 9157T was 77.02 %, confirming that strain H21T32T represents a novel species of the genus Jeotgalibaca, for which the name Jeotgalibaca ciconiae sp.
32375988	6	66	theme	w/v	753:755	arg1	NaCl					758:761	0-7 % (w/v) NaCl	746:761	0-7 % (w/v) NaCl (optimum salinity: 0.5 %)	746:787	The strain grew at 10-37 °C (optimum temperature: 30 °C), with 0-7 % (w/v) NaCl (optimum salinity: 0.5 %) and at pH 7-9 (optimum pH: 8).
32375988	2	67	theme	Oriental	225:232	arg1	stork					234:238	an Oriental stork	222:238	an Oriental stork	222:238	A novel, Gram-stain-positive, non-spore-forming, facultatively anaerobic bacterium, designated strain H21T32T, was isolated from the faeces of an Oriental stork, Ciconia boyciana.
32375988	5	68	theme	16S	491:493	arg1	sequences					505:513	The 16S rRNA gene sequences	487:513	The 16S rRNA gene sequences of the strain	487:527	The 16S rRNA gene sequences of the strain showed 97.06-97.34, 96.17-96.31 and 95.93-96.07 % similarity to the type strains of Jeotgalibaca arthritidis, J. porci and J. dankookensis, respectively.
32375988	11	69	theme	aspartic	1171:1178	arg1	acid					1180:1183	aspartic acid	1171:1183	aspartic acid	1171:1183	Amino acid components of the cell wall were l-alanine, d-glucose, l-lysine, glycine and aspartic acid.
32375988	4	70	theme	class	472:476	arg1	Bacilli					478:484	class Bacilli	472:484	class Bacilli	472:484	Strain H21T32T belonged to the genus Jeotgalibaca, family Carnobacteriaceae, order Lactobacillales and class Bacilli.
32375988	13	71	theme	strain	1345:1350	arg1	H21T32T					1352:1358	strain H21T32T	1345:1358	strain H21T32T	1345:1358	Average nucleotide identity between strain H21T32T and J. arthritidis CECT 9157T was 77.02 %, confirming that strain H21T32T represents a novel species of the genus Jeotgalibaca, for which the name Jeotgalibaca ciconiae sp.
32375988	5	72	theme	rRNA	495:498	arg1	sequences					505:513	The 16S rRNA gene sequences	487:513	The 16S rRNA gene sequences of the strain	487:527	The 16S rRNA gene sequences of the strain showed 97.06-97.34, 96.17-96.31 and 95.93-96.07 % similarity to the type strains of Jeotgalibaca arthritidis, J. porci and J. dankookensis, respectively.
32375988	10	73	theme	Sugar	1010:1014	arg1	components					1016:1025	Sugar components	1010:1025	Sugar components of the peptidoglycan	1010:1046	Sugar components of the peptidoglycan were rhamnose, ribose and glucose.
32340366	5	0	theme	palmitic	615:622	arg1	acids					624:628	oleic and palmitic acids	605:628	oleic and palmitic acids	605:628	Buriti oil is composed of oleic and palmitic acids, and the main volatile compounds were identified.
32340366	6	1	theme	buriti	684:689	arg1	oil					691:693	The buriti oil	680:693	The buriti oil	680:693	The buriti oil did not show antimicrobial activity, on the other hand, the composite gel (chitosan and oil) proved to be efficient against Staphylococcus aureus and Klebsiella pneumonia at the 10 mg/mL.
32340366	9	2	theme	chitosan-buriti	1324:1338	arg1	oil					1340:1342	the gel chitosan-buriti oil	1316:1342	the gel chitosan-buriti oil	1316:1342	The results indicated that the gel chitosan-buriti oil has a set of properties that improve its antibacterial, antioxidant and healing action, suggesting that this material can be used to treat skin lesions.
32340366	4	3	dep	antimicrobial	489:501	arg1	effects					547:553	effects	547:553	effects	547:553	Biological tests including antimicrobial, antioxidant, anti-inflammatory and healing effects were also investigated.
32340366	1	4	theme	polymeric	145:153	arg1	matrices					155:162	polymeric matrices	145:162	polymeric matrices for biomedical applications	145:190	The buriti oil (Mauritia flexuosa L.) can be associated with polymeric matrices for biomedical applications.
32340366	7	5	theme	free	1134:1137	arg1	radicals					1139:1146	free radicals	1134:1146	free radicals	1134:1146	Similar behavior was observed for antioxidant activity, determined by the β-carotene bleaching assay, composite gels presenting higher activity and buriti oil showed anti-inflammatory activity, which may be related to the inhibition of the release of free radicals.
32340366	4	6	theme	Biological	462:471	arg1	anti-inflammatory					517:533	anti-inflammatory	517:533	anti-inflammatory	517:533	Biological tests including antimicrobial, antioxidant, anti-inflammatory and healing effects were also investigated.
32340366	4	6	theme	Biological	462:471	arg1	antioxidant					504:514	antioxidant	504:514	antioxidant	504:514	Biological tests including antimicrobial, antioxidant, anti-inflammatory and healing effects were also investigated.
32340366	4	6	theme	Biological	462:471	arg1	antimicrobial					489:501	antimicrobial	489:501	antimicrobial	489:501	Biological tests including antimicrobial, antioxidant, anti-inflammatory and healing effects were also investigated.
32340366	4	6	theme	Biological	462:471	arg1	healing					539:545	healing	539:545	healing	539:545	Biological tests including antimicrobial, antioxidant, anti-inflammatory and healing effects were also investigated.
32340366	4	6	theme	Biological	462:471	arg1	tests					473:477	Biological tests	462:477	Biological tests including antimicrobial, antioxidant, anti-inflammatory and healing effects	462:553	Biological tests including antimicrobial, antioxidant, anti-inflammatory and healing effects were also investigated.
32340366	7	7	theme	anti-inflammatory	1049:1065	arg1	activity					1067:1074	anti-inflammatory activity	1049:1074	anti-inflammatory activity	1049:1074	Similar behavior was observed for antioxidant activity, determined by the β-carotene bleaching assay, composite gels presenting higher activity and buriti oil showed anti-inflammatory activity, which may be related to the inhibition of the release of free radicals.
32340366	3	8	theme	compounds	336:344	arg1	composition					346:356	The fatty acids and volatile compounds composition	307:356	The fatty acids and volatile compounds composition of buriti oil	307:370	The fatty acids and volatile compounds composition of buriti oil were performed and the composite gels were characterized using FTIR and thermal analysis.
32340366	7	9	theme	antioxidant	917:927	arg1	activity					929:936	antioxidant activity	917:936	antioxidant activity	917:936	Similar behavior was observed for antioxidant activity, determined by the β-carotene bleaching assay, composite gels presenting higher activity and buriti oil showed anti-inflammatory activity, which may be related to the inhibition of the release of free radicals.
32340366	3	10	theme	fatty	311:315	arg1	acids					317:321	fatty acids	311:321	fatty acids	311:321	The fatty acids and volatile compounds composition of buriti oil were performed and the composite gels were characterized using FTIR and thermal analysis.
32340366	7	11	theme	Similar	883:889	arg1	behavior					891:898	Similar behavior	883:898	Similar behavior	883:898	Similar behavior was observed for antioxidant activity, determined by the β-carotene bleaching assay, composite gels presenting higher activity and buriti oil showed anti-inflammatory activity, which may be related to the inhibition of the release of free radicals.
32340366	8	12	theme	faster	1251:1256	arg1	retraction					1277:1286	faster and complete wound retraction	1251:1286	faster and complete wound retraction	1251:1286	Regarding wound healing performed using in vivo testing, the composite gel (CGB) was found to promote faster and complete wound retraction.
32340366	7	13	theme	release	1123:1129	arg1	inhibition					1105:1114	the inhibition	1101:1114	the inhibition of the release of free radicals	1101:1146	Similar behavior was observed for antioxidant activity, determined by the β-carotene bleaching assay, composite gels presenting higher activity and buriti oil showed anti-inflammatory activity, which may be related to the inhibition of the release of free radicals.
32340366	9	14	theme	antioxidant	1400:1410	arg1	action					1424:1429	its antibacterial, antioxidant and healing action	1381:1429	its antibacterial, antioxidant and healing action	1381:1429	The results indicated that the gel chitosan-buriti oil has a set of properties that improve its antibacterial, antioxidant and healing action, suggesting that this material can be used to treat skin lesions.
32340366	8	15	theme	wound	1271:1275	arg1	retraction					1277:1286	faster and complete wound retraction	1251:1286	faster and complete wound retraction	1251:1286	Regarding wound healing performed using in vivo testing, the composite gel (CGB) was found to promote faster and complete wound retraction.
32340366	9	16	theme	properties	1357:1366	arg1	properties					1357:1366	properties	1357:1366	properties that improve its antibacterial, antioxidant and healing action	1357:1429	The results indicated that the gel chitosan-buriti oil has a set of properties that improve its antibacterial, antioxidant and healing action, suggesting that this material can be used to treat skin lesions.
32340366	9	16	theme	properties	1357:1366	arg1	set					1350:1352	a set	1348:1352	a set of properties that improve its antibacterial, antioxidant and healing action	1348:1429	The results indicated that the gel chitosan-buriti oil has a set of properties that improve its antibacterial, antioxidant and healing action, suggesting that this material can be used to treat skin lesions.
32340366	8	17	theme	wound	1159:1163	arg1	healing					1165:1171	wound healing	1159:1171	wound healing performed using in vivo testing	1159:1203	Regarding wound healing performed using in vivo testing, the composite gel (CGB) was found to promote faster and complete wound retraction.
32340366	8	18	theme	complete	1262:1269	arg1	retraction					1277:1286	faster and complete wound retraction	1251:1286	faster and complete wound retraction	1251:1286	Regarding wound healing performed using in vivo testing, the composite gel (CGB) was found to promote faster and complete wound retraction.
32340366	9	19	used	used	1469:1472	arg2	material					1453:1460	this material	1448:1460	this material	1448:1460	The results indicated that the gel chitosan-buriti oil has a set of properties that improve its antibacterial, antioxidant and healing action, suggesting that this material can be used to treat skin lesions.
32340366	2	20	theme	CG	250:251	arg1	effect					226:231	the effect	222:231	the effect of chitosan gel (CG) associated with buriti oil (CGB)	222:285	This study aimed to evaluate the effect of chitosan gel (CG) associated with buriti oil (CGB) as a healing agent.
32340366	2	20	theme	CG	250:251	arg1	agent					300:304	a healing agent	290:304	a healing agent	290:304	This study aimed to evaluate the effect of chitosan gel (CG) associated with buriti oil (CGB) as a healing agent.
32340366	9	21	contain	has	1344:1346	arg1	oil					1340:1342	the gel chitosan-buriti oil	1316:1342	the gel chitosan-buriti oil	1316:1342	The results indicated that the gel chitosan-buriti oil has a set of properties that improve its antibacterial, antioxidant and healing action, suggesting that this material can be used to treat skin lesions.
32340366	9	21	contain	has	1344:1346	arg2	properties					1357:1366	properties	1357:1366	properties that improve its antibacterial, antioxidant and healing action	1357:1429	The results indicated that the gel chitosan-buriti oil has a set of properties that improve its antibacterial, antioxidant and healing action, suggesting that this material can be used to treat skin lesions.
32340366	9	21	contain	has	1344:1346	arg2	set					1350:1352	a set	1348:1352	a set of properties that improve its antibacterial, antioxidant and healing action	1348:1429	The results indicated that the gel chitosan-buriti oil has a set of properties that improve its antibacterial, antioxidant and healing action, suggesting that this material can be used to treat skin lesions.
32340366	7	22	theme	bleaching	968:976	arg1	assay					978:982	the β-carotene bleaching assay	953:982	the β-carotene bleaching assay	953:982	Similar behavior was observed for antioxidant activity, determined by the β-carotene bleaching assay, composite gels presenting higher activity and buriti oil showed anti-inflammatory activity, which may be related to the inhibition of the release of free radicals.
32340366	3	23	theme	volatile	327:334	arg1	compounds					336:344	volatile compounds	327:344	volatile compounds	327:344	The fatty acids and volatile compounds composition of buriti oil were performed and the composite gels were characterized using FTIR and thermal analysis.
32340366	5	24	theme	oleic	605:609	arg1	acids					624:628	oleic and palmitic acids	605:628	oleic and palmitic acids	605:628	Buriti oil is composed of oleic and palmitic acids, and the main volatile compounds were identified.
32340366	6	25	theme	other	739:743	arg1	hand					745:748	the other hand	735:748	the other hand	735:748	The buriti oil did not show antimicrobial activity, on the other hand, the composite gel (chitosan and oil) proved to be efficient against Staphylococcus aureus and Klebsiella pneumonia at the 10 mg/mL.
32340366	1	26	theme	buriti	88:93	arg1	oil					95:97	The buriti oil	84:97	The buriti oil (Mauritia flexuosa L.)	84:120	The buriti oil (Mauritia flexuosa L.) can be associated with polymeric matrices for biomedical applications.
32340366	1	26	theme	buriti	88:93	arg1	Mauritia					100:107	Mauritia	100:107	Mauritia	100:107	The buriti oil (Mauritia flexuosa L.) can be associated with polymeric matrices for biomedical applications.
32340366	7	27	theme	composite	985:993	arg1	gels					995:998	composite gels	985:998	composite gels presenting higher activity and buriti oil	985:1040	Similar behavior was observed for antioxidant activity, determined by the β-carotene bleaching assay, composite gels presenting higher activity and buriti oil showed anti-inflammatory activity, which may be related to the inhibition of the release of free radicals.
32340366	1	28	theme	biomedical	168:177	arg1	applications					179:190	biomedical applications	168:190	biomedical applications	168:190	The buriti oil (Mauritia flexuosa L.) can be associated with polymeric matrices for biomedical applications.
32340366	0	29	theme	Biocompatible	0:12	arg1	Gels					14:17	Biocompatible Gels	0:17	Biocompatible Gels of Chitosan-Buriti Oil for Potential Wound Healing Applications.	0:82	Biocompatible Gels of Chitosan-Buriti Oil for Potential Wound Healing Applications.
32340366	7	30	theme	buriti	1031:1036	arg1	oil					1038:1040	buriti oil	1031:1040	buriti oil	1031:1040	Similar behavior was observed for antioxidant activity, determined by the β-carotene bleaching assay, composite gels presenting higher activity and buriti oil showed anti-inflammatory activity, which may be related to the inhibition of the release of free radicals.
32340366	8	31	dep	in	1189:1190	arg1	vivo					1192:1195	vivo	1192:1195	vivo	1192:1195	Regarding wound healing performed using in vivo testing, the composite gel (CGB) was found to promote faster and complete wound retraction.
32340366	8	32	theme	in	1189:1190	arg1	testing					1197:1203	in vivo testing	1189:1203	in vivo testing	1189:1203	Regarding wound healing performed using in vivo testing, the composite gel (CGB) was found to promote faster and complete wound retraction.
32340366	3	33	theme	acids	317:321	arg1	composition					346:356	The fatty acids and volatile compounds composition	307:356	The fatty acids and volatile compounds composition of buriti oil	307:370	The fatty acids and volatile compounds composition of buriti oil were performed and the composite gels were characterized using FTIR and thermal analysis.
32340366	7	34	theme	higher	1011:1016	arg1	activity					1018:1025	higher activity	1011:1025	higher activity	1011:1025	Similar behavior was observed for antioxidant activity, determined by the β-carotene bleaching assay, composite gels presenting higher activity and buriti oil showed anti-inflammatory activity, which may be related to the inhibition of the release of free radicals.
32340366	6	35	theme	composite	755:763	arg1	efficient					801:809	efficient	801:809	efficient	801:809	The buriti oil did not show antimicrobial activity, on the other hand, the composite gel (chitosan and oil) proved to be efficient against Staphylococcus aureus and Klebsiella pneumonia at the 10 mg/mL.
32340366	6	35	theme	composite	755:763	arg1	gel					765:767	the composite gel	751:767	the composite gel (chitosan and oil)	751:786	The buriti oil did not show antimicrobial activity, on the other hand, the composite gel (chitosan and oil) proved to be efficient against Staphylococcus aureus and Klebsiella pneumonia at the 10 mg/mL.
32340366	2	36	theme	buriti	270:275	arg1	oil					277:279	buriti oil	270:279	buriti oil (CGB)	270:285	This study aimed to evaluate the effect of chitosan gel (CG) associated with buriti oil (CGB) as a healing agent.
32340366	2	36	theme	buriti	270:275	arg1	CGB					282:284	CGB	282:284	CGB	282:284	This study aimed to evaluate the effect of chitosan gel (CG) associated with buriti oil (CGB) as a healing agent.
32340366	6	37	dep	gel	765:767	arg1	oil					783:785	oil	783:785	oil	783:785	The buriti oil did not show antimicrobial activity, on the other hand, the composite gel (chitosan and oil) proved to be efficient against Staphylococcus aureus and Klebsiella pneumonia at the 10 mg/mL.
32340366	6	37	dep	gel	765:767	arg1	chitosan					770:777	chitosan	770:777	chitosan	770:777	The buriti oil did not show antimicrobial activity, on the other hand, the composite gel (chitosan and oil) proved to be efficient against Staphylococcus aureus and Klebsiella pneumonia at the 10 mg/mL.
32340366	3	38	theme	buriti	361:366	arg1	oil					368:370	buriti oil	361:370	buriti oil	361:370	The fatty acids and volatile compounds composition of buriti oil were performed and the composite gels were characterized using FTIR and thermal analysis.
32340366	8	39	theme	composite	1210:1218	arg1	CGB					1225:1227	CGB	1225:1227	CGB	1225:1227	Regarding wound healing performed using in vivo testing, the composite gel (CGB) was found to promote faster and complete wound retraction.
32340366	8	39	theme	composite	1210:1218	arg1	gel					1220:1222	the composite gel	1206:1222	the composite gel (CGB)	1206:1228	Regarding wound healing performed using in vivo testing, the composite gel (CGB) was found to promote faster and complete wound retraction.
32340366	3	40	theme	composite	395:403	arg1	gels					405:408	the composite gels	391:408	the composite gels	391:408	The fatty acids and volatile compounds composition of buriti oil were performed and the composite gels were characterized using FTIR and thermal analysis.
32340366	3	41	theme	oil	368:370	arg1	composition					346:356	The fatty acids and volatile compounds composition	307:356	The fatty acids and volatile compounds composition of buriti oil	307:370	The fatty acids and volatile compounds composition of buriti oil were performed and the composite gels were characterized using FTIR and thermal analysis.
32340366	0	42	theme	Oil	38:40	arg1	Gels					14:17	Biocompatible Gels	0:17	Biocompatible Gels of Chitosan-Buriti Oil for Potential Wound Healing Applications.	0:82	Biocompatible Gels of Chitosan-Buriti Oil for Potential Wound Healing Applications.
32340366	0	43	theme	Chitosan-Buriti	22:36	arg1	Oil					38:40	Chitosan-Buriti Oil	22:40	Chitosan-Buriti Oil	22:40	Biocompatible Gels of Chitosan-Buriti Oil for Potential Wound Healing Applications.
32340366	0	44	theme	Wound	56:60	arg1	Applications					70:81	Potential Wound Healing Applications	46:81	Potential Wound Healing Applications	46:81	Biocompatible Gels of Chitosan-Buriti Oil for Potential Wound Healing Applications.
32340366	9	45	theme	antibacterial	1385:1397	arg1	action					1424:1429	its antibacterial, antioxidant and healing action	1381:1429	its antibacterial, antioxidant and healing action	1381:1429	The results indicated that the gel chitosan-buriti oil has a set of properties that improve its antibacterial, antioxidant and healing action, suggesting that this material can be used to treat skin lesions.
32340366	7	46	theme	β-carotene	957:966	arg1	assay					978:982	the β-carotene bleaching assay	953:982	the β-carotene bleaching assay	953:982	Similar behavior was observed for antioxidant activity, determined by the β-carotene bleaching assay, composite gels presenting higher activity and buriti oil showed anti-inflammatory activity, which may be related to the inhibition of the release of free radicals.
32340366	2	47	theme	healing	292:298	arg1	effect					226:231	the effect	222:231	the effect of chitosan gel (CG) associated with buriti oil (CGB)	222:285	This study aimed to evaluate the effect of chitosan gel (CG) associated with buriti oil (CGB) as a healing agent.
32340366	2	47	theme	healing	292:298	arg1	agent					300:304	a healing agent	290:304	a healing agent	290:304	This study aimed to evaluate the effect of chitosan gel (CG) associated with buriti oil (CGB) as a healing agent.
32340366	0	48	theme	Potential	46:54	arg1	Applications					70:81	Potential Wound Healing Applications	46:81	Potential Wound Healing Applications	46:81	Biocompatible Gels of Chitosan-Buriti Oil for Potential Wound Healing Applications.
32340366	5	49	theme	main	639:642	arg1	compounds					653:661	the main volatile compounds	635:661	the main volatile compounds	635:661	Buriti oil is composed of oleic and palmitic acids, and the main volatile compounds were identified.
32340366	6	50	theme	antimicrobial	708:720	arg1	activity					722:729	antimicrobial activity	708:729	antimicrobial activity	708:729	The buriti oil did not show antimicrobial activity, on the other hand, the composite gel (chitosan and oil) proved to be efficient against Staphylococcus aureus and Klebsiella pneumonia at the 10 mg/mL.
32340366	3	51	theme	thermal	444:450	arg1	analysis					452:459	FTIR and thermal analysis	435:459	FTIR and thermal analysis	435:459	The fatty acids and volatile compounds composition of buriti oil were performed and the composite gels were characterized using FTIR and thermal analysis.
32340366	5	52	theme	volatile	644:651	arg1	compounds					653:661	the main volatile compounds	635:661	the main volatile compounds	635:661	Buriti oil is composed of oleic and palmitic acids, and the main volatile compounds were identified.
32340366	5	53	theme	Buriti	579:584	arg1	oil					586:588	Buriti oil	579:588	Buriti oil	579:588	Buriti oil is composed of oleic and palmitic acids, and the main volatile compounds were identified.
32340366	9	54	theme	healing	1416:1422	arg1	action					1424:1429	its antibacterial, antioxidant and healing action	1381:1429	its antibacterial, antioxidant and healing action	1381:1429	The results indicated that the gel chitosan-buriti oil has a set of properties that improve its antibacterial, antioxidant and healing action, suggesting that this material can be used to treat skin lesions.
32340366	9	55	theme	skin	1483:1486	arg1	lesions					1488:1494	skin lesions	1483:1494	skin lesions	1483:1494	The results indicated that the gel chitosan-buriti oil has a set of properties that improve its antibacterial, antioxidant and healing action, suggesting that this material can be used to treat skin lesions.
32340366	6	56	from	mg/mL	876:880	arg1	efficient					801:809	efficient	801:809	efficient	801:809	The buriti oil did not show antimicrobial activity, on the other hand, the composite gel (chitosan and oil) proved to be efficient against Staphylococcus aureus and Klebsiella pneumonia at the 10 mg/mL.
32340366	6	56	from	mg/mL	876:880	arg1	gel					765:767	the composite gel	751:767	the composite gel (chitosan and oil)	751:786	The buriti oil did not show antimicrobial activity, on the other hand, the composite gel (chitosan and oil) proved to be efficient against Staphylococcus aureus and Klebsiella pneumonia at the 10 mg/mL.
32340366	2	57	theme	gel	245:247	arg1	CG					250:251	chitosan gel (CG)	236:252	chitosan gel (CG) associated with buriti oil (CGB)	236:285	This study aimed to evaluate the effect of chitosan gel (CG) associated with buriti oil (CGB) as a healing agent.
32340366	3	58	theme	FTIR	435:438	arg1	analysis					452:459	FTIR and thermal analysis	435:459	FTIR and thermal analysis	435:459	The fatty acids and volatile compounds composition of buriti oil were performed and the composite gels were characterized using FTIR and thermal analysis.
32340366	2	59	theme	chitosan	236:243	arg1	CG					250:251	chitosan gel (CG)	236:252	chitosan gel (CG) associated with buriti oil (CGB)	236:285	This study aimed to evaluate the effect of chitosan gel (CG) associated with buriti oil (CGB) as a healing agent.
32340366	0	60	theme	Healing	62:68	arg1	Applications					70:81	Potential Wound Healing Applications	46:81	Potential Wound Healing Applications	46:81	Biocompatible Gels of Chitosan-Buriti Oil for Potential Wound Healing Applications.
32340366	9	61	theme	gel	1320:1322	arg1	oil					1340:1342	the gel chitosan-buriti oil	1316:1342	the gel chitosan-buriti oil	1316:1342	The results indicated that the gel chitosan-buriti oil has a set of properties that improve its antibacterial, antioxidant and healing action, suggesting that this material can be used to treat skin lesions.
32340366	1	62	dep	Mauritia	100:107	arg1	L.					118:119	Mauritia flexuosa L.	100:119	Mauritia flexuosa L.	100:119	The buriti oil (Mauritia flexuosa L.) can be associated with polymeric matrices for biomedical applications.
32340366	7	63	theme	radicals	1139:1146	arg1	release					1123:1129	the release	1119:1129	the release of free radicals	1119:1146	Similar behavior was observed for antioxidant activity, determined by the β-carotene bleaching assay, composite gels presenting higher activity and buriti oil showed anti-inflammatory activity, which may be related to the inhibition of the release of free radicals.
32160015	6	0	theme	pulmonary	719:727	arg1	vessels					729:735	IPAH pulmonary vessels	714:735	IPAH pulmonary vessels	714:735	On an ultrastructural level, we observed a marked increase in BM thickness in IPAH pulmonary vessels.
32160015	10	1	theme	cell	1123:1126	arg1	adhesion					1128:1135	cell adhesion	1123:1135	cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs)	1123:1211	However, only type IV collagen concentration dependently increased cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs) and induced nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein).
32160015	6	2	theme	BM	698:699	arg1	thickness					701:709	BM thickness	698:709	BM thickness	698:709	On an ultrastructural level, we observed a marked increase in BM thickness in IPAH pulmonary vessels.
32160015	1	3	theme	several	181:187	arg1	diseases					189:196	several diseases	181:196	several diseases	181:196	The extracellular matrix (ECM) increasingly emerges as an active driver in several diseases, including idiopathic pulmonary arterial hypertension (IPAH).
32160015	1	3	theme	several	181:187	arg1	hypertension					239:250	idiopathic pulmonary arterial hypertension	209:250	idiopathic pulmonary arterial hypertension (IPAH)	209:257	The extracellular matrix (ECM) increasingly emerges as an active driver in several diseases, including idiopathic pulmonary arterial hypertension (IPAH).
32160015	7	4	theme	BM	738:739	arg1	composition					741:751	BM composition	738:751	BM composition	738:751	BM composition was distinct in small and large vessels and altered in IPAH.
32160015	1	5	theme	idiopathic	209:218	arg1	IPAH					253:256	IPAH	253:256	IPAH	253:256	The extracellular matrix (ECM) increasingly emerges as an active driver in several diseases, including idiopathic pulmonary arterial hypertension (IPAH).
32160015	1	5	theme	idiopathic	209:218	arg1	hypertension					239:250	idiopathic pulmonary arterial hypertension	209:250	idiopathic pulmonary arterial hypertension (IPAH)	209:257	The extracellular matrix (ECM) increasingly emerges as an active driver in several diseases, including idiopathic pulmonary arterial hypertension (IPAH).
32160015	1	6	theme	arterial	230:237	arg1	IPAH					253:256	IPAH	253:256	IPAH	253:256	The extracellular matrix (ECM) increasingly emerges as an active driver in several diseases, including idiopathic pulmonary arterial hypertension (IPAH).
32160015	1	6	theme	arterial	230:237	arg1	hypertension					239:250	idiopathic pulmonary arterial hypertension	209:250	idiopathic pulmonary arterial hypertension (IPAH)	209:257	The extracellular matrix (ECM) increasingly emerges as an active driver in several diseases, including idiopathic pulmonary arterial hypertension (IPAH).
32160015	12	7	theme	junction	1511:1518	arg1	formation					1520:1528	tight junction formation	1505:1528	tight junction formation	1505:1528	Accordingly, silencing of COL4A5 and LAMC1, respectively, differentially affected tight junction formation and barrier integrity in both donor and IPAH PAECs.
32160015	10	8	theme	donor	1145:1149	arg1	adhesion					1128:1135	cell adhesion	1123:1135	cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs)	1123:1211	However, only type IV collagen concentration dependently increased cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs) and induced nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein).
32160015	10	9	theme	IPAH-derived	1155:1166	arg1	PAECs					1206:1210	PAECs	1206:1210	PAECs	1206:1210	However, only type IV collagen concentration dependently increased cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs) and induced nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein).
32160015	10	9	theme	IPAH-derived	1155:1166	arg1	cells					1199:1203	IPAH-derived pulmonary arterial endothelial cells	1155:1203	IPAH-derived pulmonary arterial endothelial cells (PAECs)	1155:1211	However, only type IV collagen concentration dependently increased cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs) and induced nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein).
32160015	2	10	theme	basement	264:271	arg1	class					304:308	a specialized class	290:308	a specialized class of ECM proteins	290:324	The basement membrane (BM) is a specialized class of ECM proteins.
32160015	2	10	theme	basement	264:271	arg1	BM					283:284	BM	283:284	BM	283:284	The basement membrane (BM) is a specialized class of ECM proteins.
32160015	2	10	theme	basement	264:271	arg1	membrane					273:280	The basement membrane	260:280	The basement membrane (BM)	260:285	The basement membrane (BM) is a specialized class of ECM proteins.
32160015	11	11	theme	YAP	1404:1406	arg1	retention					1391:1399	cytoplasmic retention	1379:1399	cytoplasmic retention of YAP in IPAH PAECs	1379:1420	On the other hand, laminin caused cytoplasmic retention of YAP in IPAH PAECs.
32160015	0	12	theme	Pulmonary	73:81	arg1	Hypertension					92:103	Idiopathic Pulmonary Arterial Hypertension	62:103	Idiopathic Pulmonary Arterial Hypertension	62:103	Basement Membrane Remodeling Controls Endothelial Function in Idiopathic Pulmonary Arterial Hypertension.
32160015	3	13	from	contact	369:375	arg1	arteries					340:347	pulmonary arteries	330:347	pulmonary arteries	330:347	In pulmonary arteries, the BM is in close contact and direct proximity to vascular cells, including endothelial cells.
32160015	5	14	from	involvement	549:559	arg1	IPAH					574:577	IPAH	574:577	IPAH	574:577	Here, we aimed to shed light on the involvement of the BM in IPAH, by addressing its structure, composition, and function.
32160015	10	15	theme	cells	1199:1203	arg1	adhesion					1128:1135	cell adhesion	1123:1135	cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs)	1123:1211	However, only type IV collagen concentration dependently increased cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs) and induced nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein).
32160015	13	16	theme	BM	1654:1655	arg1	homeostasis					1657:1667	a well-maintained BM homeostasis	1636:1667	a well-maintained BM homeostasis	1636:1667	Collectively, our results highlight the importance of a well-maintained BM homeostasis.
32160015	10	17	theme	arterial	1178:1185	arg1	PAECs					1206:1210	PAECs	1206:1210	PAECs	1206:1210	However, only type IV collagen concentration dependently increased cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs) and induced nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein).
32160015	10	17	theme	arterial	1178:1185	arg1	cells					1199:1203	IPAH-derived pulmonary arterial endothelial cells	1155:1203	IPAH-derived pulmonary arterial endothelial cells (PAECs)	1155:1211	However, only type IV collagen concentration dependently increased cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs) and induced nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein).
32160015	3	18	theme	pulmonary	330:338	arg1	arteries					340:347	pulmonary arteries	330:347	pulmonary arteries	330:347	In pulmonary arteries, the BM is in close contact and direct proximity to vascular cells, including endothelial cells.
32160015	7	19	from	vessels	785:791	arg1	distinct					757:764	distinct	757:764	distinct	757:764	BM composition was distinct in small and large vessels and altered in IPAH.
32160015	11	20	theme	cytoplasmic	1379:1389	arg1	retention					1391:1399	cytoplasmic retention	1379:1399	cytoplasmic retention of YAP in IPAH PAECs	1379:1420	On the other hand, laminin caused cytoplasmic retention of YAP in IPAH PAECs.
32160015	0	21	theme	Basement	0:7	arg1	Remodeling					18:27	Basement Membrane Remodeling	0:27	Basement Membrane Remodeling	0:27	Basement Membrane Remodeling Controls Endothelial Function in Idiopathic Pulmonary Arterial Hypertension.
32160015	1	22	theme	extracellular	110:122	arg1	ECM					132:134	ECM	132:134	ECM	132:134	The extracellular matrix (ECM) increasingly emerges as an active driver in several diseases, including idiopathic pulmonary arterial hypertension (IPAH).
32160015	1	22	theme	extracellular	110:122	arg1	matrix					124:129	The extracellular matrix	106:129	The extracellular matrix (ECM)	106:135	The extracellular matrix (ECM) increasingly emerges as an active driver in several diseases, including idiopathic pulmonary arterial hypertension (IPAH).
32160015	12	23	theme	IPAH	1570:1573	arg1	PAECs					1575:1579	both donor and IPAH PAECs	1555:1579	PAECs	1575:1579	Accordingly, silencing of COL4A5 and LAMC1, respectively, differentially affected tight junction formation and barrier integrity in both donor and IPAH PAECs.
32160015	9	24	theme	endothelial	1025:1035	arg1	integrity					1045:1053	endothelial barrier integrity	1025:1053	endothelial barrier integrity	1025:1053	Type IV collagen and laminin both strengthened endothelial barrier integrity.
32160015	3	25	theme	direct	381:386	arg1	proximity					388:396	direct proximity	381:396	direct proximity	381:396	In pulmonary arteries, the BM is in close contact and direct proximity to vascular cells, including endothelial cells.
32160015	10	26	theme	nuclear	1225:1231	arg1	translocation					1233:1245	nuclear translocation	1225:1245	nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein)	1225:1342	However, only type IV collagen concentration dependently increased cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs) and induced nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein).
32160015	0	27	theme	Endothelial	38:48	arg1	Function					50:57	Endothelial Function	38:57	Endothelial Function in Idiopathic Pulmonary Arterial Hypertension	38:103	Basement Membrane Remodeling Controls Endothelial Function in Idiopathic Pulmonary Arterial Hypertension.
32160015	10	28	theme	IV	1075:1076	arg1	concentration					1087:1099	only type IV collagen concentration	1065:1099	only type IV collagen concentration	1065:1099	However, only type IV collagen concentration dependently increased cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs) and induced nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein).
32160015	12	29	theme	COL4A5	1449:1454	arg1	silencing					1436:1444	silencing	1436:1444	silencing of COL4A5 and LAMC1, respectively	1436:1478	Accordingly, silencing of COL4A5 and LAMC1, respectively, differentially affected tight junction formation and barrier integrity in both donor and IPAH PAECs.
32160015	14	30	theme	endothelial	1732:1742	arg1	function					1749:1756	altered endothelial cell function	1724:1756	altered endothelial cell function	1724:1756	By linking changes in BM structure and composition to altered endothelial cell function, we here suggest an active involvement of the BM in IPAH pathogenesis.
32160015	10	31	theme	only	1065:1068	arg1	concentration					1087:1099	only type IV collagen concentration	1065:1099	only type IV collagen concentration	1065:1099	However, only type IV collagen concentration dependently increased cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs) and induced nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein).
32160015	10	32	theme	transcriptional	1267:1281	arg1	coactivator					1283:1293	mechanosensitive transcriptional coactivator	1250:1293	mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein)	1250:1342	However, only type IV collagen concentration dependently increased cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs) and induced nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein).
32160015	2	33	theme	proteins	317:324	arg1	class					304:308	a specialized class	290:308	a specialized class of ECM proteins	290:324	The basement membrane (BM) is a specialized class of ECM proteins.
32160015	2	33	theme	proteins	317:324	arg1	membrane					273:280	The basement membrane	260:280	The basement membrane (BM)	260:285	The basement membrane (BM) is a specialized class of ECM proteins.
32160015	14	34	from	involvement	1785:1795	arg1	pathogenesis					1815:1826	IPAH pathogenesis	1810:1826	IPAH pathogenesis	1810:1826	By linking changes in BM structure and composition to altered endothelial cell function, we here suggest an active involvement of the BM in IPAH pathogenesis.
32160015	9	35	dep	strengthened	1012:1023	arg1	both					1007:1010	both	1007:1010	both	1007:1010	Type IV collagen and laminin both strengthened endothelial barrier integrity.
32160015	10	36	theme	YAP	1316:1318	arg1	coactivator					1283:1293	mechanosensitive transcriptional coactivator	1250:1293	mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein)	1250:1342	However, only type IV collagen concentration dependently increased cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs) and induced nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein).
32160015	14	37	theme	BM	1692:1693	arg1	structure					1695:1703	BM structure	1692:1703	BM structure	1692:1703	By linking changes in BM structure and composition to altered endothelial cell function, we here suggest an active involvement of the BM in IPAH pathogenesis.
32160015	8	38	theme	smaller	876:882	arg1	vessels					895:901	smaller and larger vessels	876:901	smaller and larger vessels	876:901	Proteoglycans were mostly responsible for distinction between smaller and larger vessels, whereas BM collagens and laminins were more abundantly expressed in IPAH.
32160015	14	39	theme	altered	1724:1730	arg1	function					1749:1756	altered endothelial cell function	1724:1756	altered endothelial cell function	1724:1756	By linking changes in BM structure and composition to altered endothelial cell function, we here suggest an active involvement of the BM in IPAH pathogenesis.
32160015	10	40	theme	hippo	1302:1306	arg1	YAP					1316:1318	the hippo pathway YAP	1298:1318	the hippo pathway YAP (Yes-activated protein)	1298:1342	However, only type IV collagen concentration dependently increased cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs) and induced nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein).
32160015	10	40	theme	hippo	1302:1306	arg1	protein					1335:1341	Yes-activated protein	1321:1341	Yes-activated protein	1321:1341	However, only type IV collagen concentration dependently increased cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs) and induced nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein).
32160015	8	41	theme	larger	888:893	arg1	vessels					895:901	smaller and larger vessels	876:901	smaller and larger vessels	876:901	Proteoglycans were mostly responsible for distinction between smaller and larger vessels, whereas BM collagens and laminins were more abundantly expressed in IPAH.
32160015	11	42	theme	other	1352:1356	arg1	hand					1358:1361	the other hand	1348:1361	the other hand	1348:1361	On the other hand, laminin caused cytoplasmic retention of YAP in IPAH PAECs.
32160015	6	43	theme	IPAH	714:717	arg1	vessels					729:735	IPAH pulmonary vessels	714:735	IPAH pulmonary vessels	714:735	On an ultrastructural level, we observed a marked increase in BM thickness in IPAH pulmonary vessels.
32160015	7	44	theme	large	779:783	arg1	vessels					785:791	small and large vessels	769:791	small and large vessels	769:791	BM composition was distinct in small and large vessels and altered in IPAH.
32160015	10	45	theme	Yes-activated	1321:1333	arg1	YAP					1316:1318	the hippo pathway YAP	1298:1318	the hippo pathway YAP (Yes-activated protein)	1298:1342	However, only type IV collagen concentration dependently increased cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs) and induced nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein).
32160015	10	45	theme	Yes-activated	1321:1333	arg1	protein					1335:1341	Yes-activated protein	1321:1341	Yes-activated protein	1321:1341	However, only type IV collagen concentration dependently increased cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs) and induced nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein).
32160015	7	46	theme	small	769:773	arg1	vessels					785:791	small and large vessels	769:791	small and large vessels	769:791	BM composition was distinct in small and large vessels and altered in IPAH.
32160015	8	47	theme	BM	912:913	arg1	collagens					915:923	BM collagens	912:923	BM collagens	912:923	Proteoglycans were mostly responsible for distinction between smaller and larger vessels, whereas BM collagens and laminins were more abundantly expressed in IPAH.
32160015	5	48	theme	BM	568:569	arg1	involvement					549:559	the involvement	545:559	the involvement of the BM in IPAH	545:577	Here, we aimed to shed light on the involvement of the BM in IPAH, by addressing its structure, composition, and function.
32160015	6	49	theme	marked	679:684	arg1	increase					686:693	a marked increase	677:693	a marked increase in BM thickness in IPAH pulmonary vessels	677:735	On an ultrastructural level, we observed a marked increase in BM thickness in IPAH pulmonary vessels.
32160015	2	50	theme	specialized	292:302	arg1	class					304:308	a specialized class	290:308	a specialized class of ECM proteins	290:324	The basement membrane (BM) is a specialized class of ECM proteins.
32160015	2	50	theme	specialized	292:302	arg1	membrane					273:280	The basement membrane	260:280	The basement membrane (BM)	260:285	The basement membrane (BM) is a specialized class of ECM proteins.
32160015	12	51	theme	donor	1560:1564	arg1	PAECs					1575:1579	both donor and IPAH PAECs	1555:1579	PAECs	1575:1579	Accordingly, silencing of COL4A5 and LAMC1, respectively, differentially affected tight junction formation and barrier integrity in both donor and IPAH PAECs.
32160015	14	52	theme	IPAH	1810:1813	arg1	pathogenesis					1815:1826	IPAH pathogenesis	1810:1826	IPAH pathogenesis	1810:1826	By linking changes in BM structure and composition to altered endothelial cell function, we here suggest an active involvement of the BM in IPAH pathogenesis.
32160015	14	53	theme	BM	1804:1805	arg1	involvement					1785:1795	an active involvement	1775:1795	an active involvement of the BM in IPAH pathogenesis	1775:1826	By linking changes in BM structure and composition to altered endothelial cell function, we here suggest an active involvement of the BM in IPAH pathogenesis.
32160015	12	54	theme	barrier	1534:1540	arg1	integrity					1542:1550	barrier integrity	1534:1550	barrier integrity	1534:1550	Accordingly, silencing of COL4A5 and LAMC1, respectively, differentially affected tight junction formation and barrier integrity in both donor and IPAH PAECs.
32160015	10	55	theme	pulmonary	1168:1176	arg1	PAECs					1206:1210	PAECs	1206:1210	PAECs	1206:1210	However, only type IV collagen concentration dependently increased cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs) and induced nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein).
32160015	10	55	theme	pulmonary	1168:1176	arg1	cells					1199:1203	IPAH-derived pulmonary arterial endothelial cells	1155:1203	IPAH-derived pulmonary arterial endothelial cells (PAECs)	1155:1211	However, only type IV collagen concentration dependently increased cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs) and induced nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein).
32160015	11	56	theme	IPAH	1411:1414	arg1	PAECs					1416:1420	IPAH PAECs	1411:1420	IPAH PAECs	1411:1420	On the other hand, laminin caused cytoplasmic retention of YAP in IPAH PAECs.
32160015	0	57	theme	Arterial	83:90	arg1	Hypertension					92:103	Idiopathic Pulmonary Arterial Hypertension	62:103	Idiopathic Pulmonary Arterial Hypertension	62:103	Basement Membrane Remodeling Controls Endothelial Function in Idiopathic Pulmonary Arterial Hypertension.
32160015	14	58	theme	active	1778:1783	arg1	involvement					1785:1795	an active involvement	1775:1795	an active involvement of the BM in IPAH pathogenesis	1775:1826	By linking changes in BM structure and composition to altered endothelial cell function, we here suggest an active involvement of the BM in IPAH pathogenesis.
32160015	12	59	theme	tight	1505:1509	arg1	formation					1520:1528	tight junction formation	1505:1528	tight junction formation	1505:1528	Accordingly, silencing of COL4A5 and LAMC1, respectively, differentially affected tight junction formation and barrier integrity in both donor and IPAH PAECs.
32160015	1	60	theme	active	164:169	arg1	driver					171:176	an active driver	161:176	an active driver in several diseases, including idiopathic pulmonary arterial hypertension (IPAH)	161:257	The extracellular matrix (ECM) increasingly emerges as an active driver in several diseases, including idiopathic pulmonary arterial hypertension (IPAH).
32160015	12	61	theme	LAMC1	1460:1464	arg1	silencing					1436:1444	silencing	1436:1444	silencing of COL4A5 and LAMC1, respectively	1436:1478	Accordingly, silencing of COL4A5 and LAMC1, respectively, differentially affected tight junction formation and barrier integrity in both donor and IPAH PAECs.
32160015	4	62	theme	BM	470:471	arg1	role					458:461	the role	454:461	the role of the BM	454:471	So far, the role of the BM has remained underinvestigated in IPAH.
32160015	13	63	theme	well-maintained	1638:1652	arg1	homeostasis					1657:1667	a well-maintained BM homeostasis	1636:1667	a well-maintained BM homeostasis	1636:1667	Collectively, our results highlight the importance of a well-maintained BM homeostasis.
32160015	10	64	link	IPAH-derived	1155:1166	arg1	PAECs					1206:1210	PAECs	1206:1210	PAECs	1206:1210	However, only type IV collagen concentration dependently increased cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs) and induced nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein).
32160015	10	64	link	IPAH-derived	1155:1166	arg1	cells					1199:1203	IPAH-derived pulmonary arterial endothelial cells	1155:1203	IPAH-derived pulmonary arterial endothelial cells (PAECs)	1155:1211	However, only type IV collagen concentration dependently increased cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs) and induced nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein).
32160015	10	65	theme	endothelial	1187:1197	arg1	PAECs					1206:1210	PAECs	1206:1210	PAECs	1206:1210	However, only type IV collagen concentration dependently increased cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs) and induced nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein).
32160015	10	65	theme	endothelial	1187:1197	arg1	cells					1199:1203	IPAH-derived pulmonary arterial endothelial cells	1155:1203	IPAH-derived pulmonary arterial endothelial cells (PAECs)	1155:1211	However, only type IV collagen concentration dependently increased cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs) and induced nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein).
32160015	3	66	from	proximity	388:396	arg1	arteries					340:347	pulmonary arteries	330:347	pulmonary arteries	330:347	In pulmonary arteries, the BM is in close contact and direct proximity to vascular cells, including endothelial cells.
32160015	1	67	from	driver	171:176	arg1	diseases					189:196	several diseases	181:196	several diseases	181:196	The extracellular matrix (ECM) increasingly emerges as an active driver in several diseases, including idiopathic pulmonary arterial hypertension (IPAH).
32160015	1	67	from	driver	171:176	arg1	hypertension					239:250	idiopathic pulmonary arterial hypertension	209:250	idiopathic pulmonary arterial hypertension (IPAH)	209:257	The extracellular matrix (ECM) increasingly emerges as an active driver in several diseases, including idiopathic pulmonary arterial hypertension (IPAH).
32160015	13	68	theme	homeostasis	1657:1667	arg1	importance					1622:1631	the importance	1618:1631	the importance of a well-maintained BM homeostasis	1618:1667	Collectively, our results highlight the importance of a well-maintained BM homeostasis.
32160015	9	69	theme	Type	978:981	arg1	collagen					986:993	Type IV collagen	978:993	Type IV collagen	978:993	Type IV collagen and laminin both strengthened endothelial barrier integrity.
32160015	14	70	from	changes	1681:1687	arg1	composition					1709:1719	composition	1709:1719	composition	1709:1719	By linking changes in BM structure and composition to altered endothelial cell function, we here suggest an active involvement of the BM in IPAH pathogenesis.
32160015	14	70	from	changes	1681:1687	arg1	structure					1695:1703	BM structure	1692:1703	BM structure	1692:1703	By linking changes in BM structure and composition to altered endothelial cell function, we here suggest an active involvement of the BM in IPAH pathogenesis.
32160015	0	71	from	Function	50:57	arg1	Hypertension					92:103	Idiopathic Pulmonary Arterial Hypertension	62:103	Idiopathic Pulmonary Arterial Hypertension	62:103	Basement Membrane Remodeling Controls Endothelial Function in Idiopathic Pulmonary Arterial Hypertension.
32160015	0	72	theme	Membrane	9:16	arg1	Remodeling					18:27	Basement Membrane Remodeling	0:27	Basement Membrane Remodeling	0:27	Basement Membrane Remodeling Controls Endothelial Function in Idiopathic Pulmonary Arterial Hypertension.
32160015	3	73	theme	close	363:367	arg1	contact					369:375	close contact	363:375	close contact	363:375	In pulmonary arteries, the BM is in close contact and direct proximity to vascular cells, including endothelial cells.
32160015	10	74	theme	mechanosensitive	1250:1265	arg1	coactivator					1283:1293	mechanosensitive transcriptional coactivator	1250:1293	mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein)	1250:1342	However, only type IV collagen concentration dependently increased cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs) and induced nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein).
32160015	3	75	theme	vascular	401:408	arg1	cells					410:414	vascular cells	401:414	vascular cells	401:414	In pulmonary arteries, the BM is in close contact and direct proximity to vascular cells, including endothelial cells.
32160015	3	75	theme	vascular	401:408	arg1	cells					439:443	endothelial cells	427:443	endothelial cells	427:443	In pulmonary arteries, the BM is in close contact and direct proximity to vascular cells, including endothelial cells.
32160015	6	76	from	increase	686:693	arg1	vessels					729:735	IPAH pulmonary vessels	714:735	IPAH pulmonary vessels	714:735	On an ultrastructural level, we observed a marked increase in BM thickness in IPAH pulmonary vessels.
32160015	6	76	from	increase	686:693	arg1	thickness					701:709	BM thickness	698:709	BM thickness	698:709	On an ultrastructural level, we observed a marked increase in BM thickness in IPAH pulmonary vessels.
32160015	6	77	theme	ultrastructural	642:656	arg1	level					658:662	an ultrastructural level	639:662	an ultrastructural level	639:662	On an ultrastructural level, we observed a marked increase in BM thickness in IPAH pulmonary vessels.
32160015	14	78	theme	cell	1744:1747	arg1	function					1749:1756	altered endothelial cell function	1724:1756	altered endothelial cell function	1724:1756	By linking changes in BM structure and composition to altered endothelial cell function, we here suggest an active involvement of the BM in IPAH pathogenesis.
32160015	1	79	theme	pulmonary	220:228	arg1	IPAH					253:256	IPAH	253:256	IPAH	253:256	The extracellular matrix (ECM) increasingly emerges as an active driver in several diseases, including idiopathic pulmonary arterial hypertension (IPAH).
32160015	1	79	theme	pulmonary	220:228	arg1	hypertension					239:250	idiopathic pulmonary arterial hypertension	209:250	idiopathic pulmonary arterial hypertension (IPAH)	209:257	The extracellular matrix (ECM) increasingly emerges as an active driver in several diseases, including idiopathic pulmonary arterial hypertension (IPAH).
32160015	11	80	from	retention	1391:1399	arg1	PAECs					1416:1420	IPAH PAECs	1411:1420	IPAH PAECs	1411:1420	On the other hand, laminin caused cytoplasmic retention of YAP in IPAH PAECs.
32160015	9	81	theme	barrier	1037:1043	arg1	integrity					1045:1053	endothelial barrier integrity	1025:1053	endothelial barrier integrity	1025:1053	Type IV collagen and laminin both strengthened endothelial barrier integrity.
32160015	3	82	theme	endothelial	427:437	arg1	cells					439:443	endothelial cells	427:443	endothelial cells	427:443	In pulmonary arteries, the BM is in close contact and direct proximity to vascular cells, including endothelial cells.
32160015	0	83	theme	Idiopathic	62:71	arg1	Hypertension					92:103	Idiopathic Pulmonary Arterial Hypertension	62:103	Idiopathic Pulmonary Arterial Hypertension	62:103	Basement Membrane Remodeling Controls Endothelial Function in Idiopathic Pulmonary Arterial Hypertension.
32160015	10	84	theme	type	1070:1073	arg1	concentration					1087:1099	only type IV collagen concentration	1065:1099	only type IV collagen concentration	1065:1099	However, only type IV collagen concentration dependently increased cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs) and induced nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein).
32160015	10	85	theme	coactivator	1283:1293	arg1	translocation					1233:1245	nuclear translocation	1225:1245	nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein)	1225:1342	However, only type IV collagen concentration dependently increased cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs) and induced nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein).
32160015	7	86	from	distinct	757:764	arg1	vessels					785:791	small and large vessels	769:791	small and large vessels	769:791	BM composition was distinct in small and large vessels and altered in IPAH.
32160015	2	87	theme	ECM	313:315	arg1	proteins					317:324	ECM proteins	313:324	ECM proteins	313:324	The basement membrane (BM) is a specialized class of ECM proteins.
32160015	10	88	theme	pathway	1308:1314	arg1	YAP					1316:1318	the hippo pathway YAP	1298:1318	the hippo pathway YAP (Yes-activated protein)	1298:1342	However, only type IV collagen concentration dependently increased cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs) and induced nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein).
32160015	10	88	theme	pathway	1308:1314	arg1	protein					1335:1341	Yes-activated protein	1321:1341	Yes-activated protein	1321:1341	However, only type IV collagen concentration dependently increased cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs) and induced nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein).
32160015	10	89	theme	collagen	1078:1085	arg1	concentration					1087:1099	only type IV collagen concentration	1065:1099	only type IV collagen concentration	1065:1099	However, only type IV collagen concentration dependently increased cell adhesion of both donor and IPAH-derived pulmonary arterial endothelial cells (PAECs) and induced nuclear translocation of mechanosensitive transcriptional coactivator of the hippo pathway YAP (Yes-activated protein).
32701080	8	0	from	alteration	1183:1192	arg1	mice					1227:1230	colitic mice	1219:1230	colitic mice	1219:1230	Furthermore, SH partially restored the alteration of the gut microbiota in colitic mice.
32701080	7	1	theme	MAPK	1116:1119	arg1	pathways					1121:1128	MAPK pathways	1116:1128	MAPK pathways in the colon	1116:1141	Western blotting results revealed that SH suppressed DSS-induced activation of the NF-κB and MAPK pathways in the colon.
32701080	7	2	theme	Western	1023:1029	arg1	blotting					1031:1038	Western blotting	1023:1038	Western blotting	1023:1038	Western blotting results revealed that SH suppressed DSS-induced activation of the NF-κB and MAPK pathways in the colon.
32701080	3	3	theme	much	502:505	arg1	promise					507:513	much promise	502:513	much promise	502:513	Studying the biological activities of sturgeon muscle-derived hydrolysates holds much promise for adding value to sturgeon.
32701080	9	4	theme	bacteria	1426:1433	arg1	abundance					1393:1401	the abundance	1389:1401	the abundance of potentially harmful bacteria Erysipelotrichaceae and Enterococcaceae	1389:1473	SH increased the Bacteroidetes/Firmicutes ratio and the relative abundance of Ruminococcaceae, Porphyromonadaceae, and Bacteroidetes S24-7, while decreased the abundance of potentially harmful bacteria Erysipelotrichaceae and Enterococcaceae.
32701080	2	5	attach	derived	320:326	arg2	hydrolysates					293:304	dietary protein hydrolysates	277:304	dietary protein hydrolysates	277:304	Yet dietary protein hydrolysates, which may be derived from sturgeon muscle, have been reported to have versatile beneficial biological activities.
32701080	2	5	attach	derived	320:326	arg1	muscle					342:347	sturgeon muscle	333:347	sturgeon muscle	333:347	Yet dietary protein hydrolysates, which may be derived from sturgeon muscle, have been reported to have versatile beneficial biological activities.
32701080	9	6	dep	bacteria	1426:1433	arg1	Erysipelotrichaceae					1435:1453	Erysipelotrichaceae	1435:1453	Erysipelotrichaceae	1435:1453	SH increased the Bacteroidetes/Firmicutes ratio and the relative abundance of Ruminococcaceae, Porphyromonadaceae, and Bacteroidetes S24-7, while decreased the abundance of potentially harmful bacteria Erysipelotrichaceae and Enterococcaceae.
32701080	9	6	dep	bacteria	1426:1433	arg1	bacteria					1426:1433	potentially harmful bacteria	1406:1433	potentially harmful bacteria Erysipelotrichaceae and Enterococcaceae	1406:1473	SH increased the Bacteroidetes/Firmicutes ratio and the relative abundance of Ruminococcaceae, Porphyromonadaceae, and Bacteroidetes S24-7, while decreased the abundance of potentially harmful bacteria Erysipelotrichaceae and Enterococcaceae.
32701080	9	6	dep	bacteria	1426:1433	arg1	Enterococcaceae					1459:1473	Enterococcaceae	1459:1473	Enterococcaceae	1459:1473	SH increased the Bacteroidetes/Firmicutes ratio and the relative abundance of Ruminococcaceae, Porphyromonadaceae, and Bacteroidetes S24-7, while decreased the abundance of potentially harmful bacteria Erysipelotrichaceae and Enterococcaceae.
32701080	1	7	theme	caviar	163:168	arg1	production					170:179	caviar production	163:179	caviar production	163:179	Sturgeon muscle byproduct collected after caviar production is usually not fully utilized, and sometimes may be discarded, thus causing a lot of waste.
32701080	6	8	theme	IL-6	999:1002	arg1	levels					989:994	the serum levels	979:994	the serum levels of IL-6, IL-1β, and TNF-α	979:1020	SH also inhibited myeloperoxidase (MPO) activity and reduced the serum levels of IL-6, IL-1β, and TNF-α.
32701080	10	9	theme	NF-κB	1554:1558	arg1	pathways					1569:1576	the NF-κB and MAPK pathways	1550:1576	the NF-κB and MAPK pathways	1550:1576	These results suggest that SH inhibited DSS-induced colitis by regulating the NF-κB and MAPK pathways and modulating microbiota composition.
32701080	7	10	theme	NF-κB	1106:1110	arg1	activation					1088:1097	DSS-induced activation	1076:1097	DSS-induced activation of the NF-κB	1076:1110	Western blotting results revealed that SH suppressed DSS-induced activation of the NF-κB and MAPK pathways in the colon.
32701080	7	10	theme	NF-κB	1106:1110	arg1	pathways					1121:1128	MAPK pathways	1116:1128	MAPK pathways in the colon	1116:1141	Western blotting results revealed that SH suppressed DSS-induced activation of the NF-κB and MAPK pathways in the colon.
32701080	5	11	theme	histological	897:908	arg1	scores					910:915	histological scores	897:915	histological scores	897:915	The administration of sturgeon hydrolysates (SH) significantly decreased the severity of DSS-induced damage, evidenced by increased body weight, colon length, and decreased disease activity index (DAI) and histological scores.
32701080	4	12	theme	therapeutic	582:592	arg1	effects					612:618	the therapeutic anti-inflammatory effects	578:618	the therapeutic anti-inflammatory effects of sturgeon muscle-derived hydrolysates	578:658	The current study aimed to study the therapeutic anti-inflammatory effects of sturgeon muscle-derived hydrolysates and the underlying mechanisms.
32701080	3	13	theme	muscle-derived	468:481	arg1	hydrolysates					483:494	sturgeon muscle-derived hydrolysates	459:494	sturgeon muscle-derived hydrolysates	459:494	Studying the biological activities of sturgeon muscle-derived hydrolysates holds much promise for adding value to sturgeon.
32701080	0	14	theme	microbiota	97:106	arg1	composition					108:118	microbiota composition	97:118	microbiota composition	97:118	Sturgeon hydrolysates alleviate DSS-induced colon colitis in mice by modulating NF-κB, MAPK, and microbiota composition.
32701080	9	15	theme	Ruminococcaceae	1311:1325	arg1	abundance					1298:1306	the relative abundance	1285:1306	the relative abundance of Ruminococcaceae, Porphyromonadaceae, and Bacteroidetes S24-7	1285:1370	SH increased the Bacteroidetes/Firmicutes ratio and the relative abundance of Ruminococcaceae, Porphyromonadaceae, and Bacteroidetes S24-7, while decreased the abundance of potentially harmful bacteria Erysipelotrichaceae and Enterococcaceae.
32701080	9	15	theme	Ruminococcaceae	1311:1325	arg1	ratio					1275:1279	the Bacteroidetes/Firmicutes ratio	1246:1279	the Bacteroidetes/Firmicutes ratio	1246:1279	SH increased the Bacteroidetes/Firmicutes ratio and the relative abundance of Ruminococcaceae, Porphyromonadaceae, and Bacteroidetes S24-7, while decreased the abundance of potentially harmful bacteria Erysipelotrichaceae and Enterococcaceae.
32701080	5	16	theme	decreased	854:862	arg1	DAI					888:890	DAI	888:890	DAI	888:890	The administration of sturgeon hydrolysates (SH) significantly decreased the severity of DSS-induced damage, evidenced by increased body weight, colon length, and decreased disease activity index (DAI) and histological scores.
32701080	5	16	theme	decreased	854:862	arg1	index					881:885	decreased disease activity index	854:885	decreased disease activity index (DAI)	854:891	The administration of sturgeon hydrolysates (SH) significantly decreased the severity of DSS-induced damage, evidenced by increased body weight, colon length, and decreased disease activity index (DAI) and histological scores.
32701080	4	17	theme	muscle-derived	632:645	arg1	hydrolysates					647:658	sturgeon muscle-derived hydrolysates	623:658	sturgeon muscle-derived hydrolysates	623:658	The current study aimed to study the therapeutic anti-inflammatory effects of sturgeon muscle-derived hydrolysates and the underlying mechanisms.
32701080	9	18	theme	relative	1289:1296	arg1	abundance					1298:1306	the relative abundance	1285:1306	the relative abundance of Ruminococcaceae, Porphyromonadaceae, and Bacteroidetes S24-7	1285:1370	SH increased the Bacteroidetes/Firmicutes ratio and the relative abundance of Ruminococcaceae, Porphyromonadaceae, and Bacteroidetes S24-7, while decreased the abundance of potentially harmful bacteria Erysipelotrichaceae and Enterococcaceae.
32701080	7	19	dep	results	1040:1046	arg1	revealed					1048:1055	revealed	1048:1055	results revealed that SH suppressed DSS-induced activation of the NF-κB and MAPK pathways in the colon	1040:1141	Western blotting results revealed that SH suppressed DSS-induced activation of the NF-κB and MAPK pathways in the colon.
32701080	0	20	from	colitis	50:56	arg1	mice					61:64	mice	61:64	mice	61:64	Sturgeon hydrolysates alleviate DSS-induced colon colitis in mice by modulating NF-κB, MAPK, and microbiota composition.
32701080	4	21	theme	anti-inflammatory	594:610	arg1	effects					612:618	the therapeutic anti-inflammatory effects	578:618	the therapeutic anti-inflammatory effects of sturgeon muscle-derived hydrolysates	578:658	The current study aimed to study the therapeutic anti-inflammatory effects of sturgeon muscle-derived hydrolysates and the underlying mechanisms.
32701080	4	22	theme	underlying	668:677	arg1	mechanisms					679:688	the underlying mechanisms	664:688	the underlying mechanisms	664:688	The current study aimed to study the therapeutic anti-inflammatory effects of sturgeon muscle-derived hydrolysates and the underlying mechanisms.
32701080	9	23	theme	Porphyromonadaceae	1328:1345	arg1	abundance					1298:1306	the relative abundance	1285:1306	the relative abundance of Ruminococcaceae, Porphyromonadaceae, and Bacteroidetes S24-7	1285:1370	SH increased the Bacteroidetes/Firmicutes ratio and the relative abundance of Ruminococcaceae, Porphyromonadaceae, and Bacteroidetes S24-7, while decreased the abundance of potentially harmful bacteria Erysipelotrichaceae and Enterococcaceae.
32701080	9	23	theme	Porphyromonadaceae	1328:1345	arg1	ratio					1275:1279	the Bacteroidetes/Firmicutes ratio	1246:1279	the Bacteroidetes/Firmicutes ratio	1246:1279	SH increased the Bacteroidetes/Firmicutes ratio and the relative abundance of Ruminococcaceae, Porphyromonadaceae, and Bacteroidetes S24-7, while decreased the abundance of potentially harmful bacteria Erysipelotrichaceae and Enterococcaceae.
32701080	7	24	theme	DSS-induced	1076:1086	arg1	activation					1088:1097	DSS-induced activation	1076:1097	DSS-induced activation of the NF-κB	1076:1110	Western blotting results revealed that SH suppressed DSS-induced activation of the NF-κB and MAPK pathways in the colon.
32701080	6	25	theme	serum	983:987	arg1	levels					989:994	the serum levels	979:994	the serum levels of IL-6, IL-1β, and TNF-α	979:1020	SH also inhibited myeloperoxidase (MPO) activity and reduced the serum levels of IL-6, IL-1β, and TNF-α.
32701080	10	26	theme	MAPK	1564:1567	arg1	pathways					1569:1576	the NF-κB and MAPK pathways	1550:1576	the NF-κB and MAPK pathways	1550:1576	These results suggest that SH inhibited DSS-induced colitis by regulating the NF-κB and MAPK pathways and modulating microbiota composition.
32701080	0	27	theme	Sturgeon	0:7	arg1	hydrolysates					9:20	Sturgeon hydrolysates	0:20	Sturgeon hydrolysates	0:20	Sturgeon hydrolysates alleviate DSS-induced colon colitis in mice by modulating NF-κB, MAPK, and microbiota composition.
32701080	4	28	theme	current	549:555	arg1	study					557:561	The current study	545:561	The current study	545:561	The current study aimed to study the therapeutic anti-inflammatory effects of sturgeon muscle-derived hydrolysates and the underlying mechanisms.
32701080	2	29	theme	protein	285:291	arg1	hydrolysates					293:304	dietary protein hydrolysates	277:304	dietary protein hydrolysates	277:304	Yet dietary protein hydrolysates, which may be derived from sturgeon muscle, have been reported to have versatile beneficial biological activities.
32701080	9	30	theme	harmful	1418:1424	arg1	Erysipelotrichaceae					1435:1453	Erysipelotrichaceae	1435:1453	Erysipelotrichaceae	1435:1453	SH increased the Bacteroidetes/Firmicutes ratio and the relative abundance of Ruminococcaceae, Porphyromonadaceae, and Bacteroidetes S24-7, while decreased the abundance of potentially harmful bacteria Erysipelotrichaceae and Enterococcaceae.
32701080	9	30	theme	harmful	1418:1424	arg1	bacteria					1426:1433	potentially harmful bacteria	1406:1433	potentially harmful bacteria Erysipelotrichaceae and Enterococcaceae	1406:1473	SH increased the Bacteroidetes/Firmicutes ratio and the relative abundance of Ruminococcaceae, Porphyromonadaceae, and Bacteroidetes S24-7, while decreased the abundance of potentially harmful bacteria Erysipelotrichaceae and Enterococcaceae.
32701080	9	30	theme	harmful	1418:1424	arg1	Enterococcaceae					1459:1473	Enterococcaceae	1459:1473	Enterococcaceae	1459:1473	SH increased the Bacteroidetes/Firmicutes ratio and the relative abundance of Ruminococcaceae, Porphyromonadaceae, and Bacteroidetes S24-7, while decreased the abundance of potentially harmful bacteria Erysipelotrichaceae and Enterococcaceae.
32701080	0	31	theme	DSS-induced	32:42	arg1	colitis					50:56	DSS-induced colon colitis	32:56	DSS-induced colon colitis in mice	32:64	Sturgeon hydrolysates alleviate DSS-induced colon colitis in mice by modulating NF-κB, MAPK, and microbiota composition.
32701080	5	32	theme	colon	836:840	arg1	length					842:847	colon length	836:847	colon length	836:847	The administration of sturgeon hydrolysates (SH) significantly decreased the severity of DSS-induced damage, evidenced by increased body weight, colon length, and decreased disease activity index (DAI) and histological scores.
32701080	2	33	theme	dietary	277:283	arg1	hydrolysates					293:304	dietary protein hydrolysates	277:304	dietary protein hydrolysates	277:304	Yet dietary protein hydrolysates, which may be derived from sturgeon muscle, have been reported to have versatile beneficial biological activities.
32701080	2	34	theme	beneficial	387:396	arg1	activities					409:418	versatile beneficial biological activities	377:418	versatile beneficial biological activities	377:418	Yet dietary protein hydrolysates, which may be derived from sturgeon muscle, have been reported to have versatile beneficial biological activities.
32701080	2	35	theme	biological	398:407	arg1	activities					409:418	versatile beneficial biological activities	377:418	versatile beneficial biological activities	377:418	Yet dietary protein hydrolysates, which may be derived from sturgeon muscle, have been reported to have versatile beneficial biological activities.
32701080	1	36	theme	waste	266:270	arg1	waste					266:270	waste	266:270	waste	266:270	Sturgeon muscle byproduct collected after caviar production is usually not fully utilized, and sometimes may be discarded, thus causing a lot of waste.
32701080	1	36	theme	waste	266:270	arg1	lot					259:261	a lot	257:261	a lot of waste	257:270	Sturgeon muscle byproduct collected after caviar production is usually not fully utilized, and sometimes may be discarded, thus causing a lot of waste.
32701080	10	37	theme	microbiota	1593:1602	arg1	composition					1604:1614	microbiota composition	1593:1614	microbiota composition	1593:1614	These results suggest that SH inhibited DSS-induced colitis by regulating the NF-κB and MAPK pathways and modulating microbiota composition.
32701080	2	38	theme	versatile	377:385	arg1	activities					409:418	versatile beneficial biological activities	377:418	versatile beneficial biological activities	377:418	Yet dietary protein hydrolysates, which may be derived from sturgeon muscle, have been reported to have versatile beneficial biological activities.
32701080	8	39	theme	gut	1201:1203	arg1	microbiota					1205:1214	the gut microbiota	1197:1214	the gut microbiota	1197:1214	Furthermore, SH partially restored the alteration of the gut microbiota in colitic mice.
32701080	9	40	theme	S24-7	1366:1370	arg1	abundance					1298:1306	the relative abundance	1285:1306	the relative abundance of Ruminococcaceae, Porphyromonadaceae, and Bacteroidetes S24-7	1285:1370	SH increased the Bacteroidetes/Firmicutes ratio and the relative abundance of Ruminococcaceae, Porphyromonadaceae, and Bacteroidetes S24-7, while decreased the abundance of potentially harmful bacteria Erysipelotrichaceae and Enterococcaceae.
32701080	9	40	theme	S24-7	1366:1370	arg1	ratio					1275:1279	the Bacteroidetes/Firmicutes ratio	1246:1279	the Bacteroidetes/Firmicutes ratio	1246:1279	SH increased the Bacteroidetes/Firmicutes ratio and the relative abundance of Ruminococcaceae, Porphyromonadaceae, and Bacteroidetes S24-7, while decreased the abundance of potentially harmful bacteria Erysipelotrichaceae and Enterococcaceae.
32701080	5	41	theme	disease	864:870	arg1	DAI					888:890	DAI	888:890	DAI	888:890	The administration of sturgeon hydrolysates (SH) significantly decreased the severity of DSS-induced damage, evidenced by increased body weight, colon length, and decreased disease activity index (DAI) and histological scores.
32701080	5	41	theme	disease	864:870	arg1	index					881:885	decreased disease activity index	854:885	decreased disease activity index (DAI)	854:891	The administration of sturgeon hydrolysates (SH) significantly decreased the severity of DSS-induced damage, evidenced by increased body weight, colon length, and decreased disease activity index (DAI) and histological scores.
32701080	0	42	theme	colon	44:48	arg1	colitis					50:56	DSS-induced colon colitis	32:56	DSS-induced colon colitis in mice	32:64	Sturgeon hydrolysates alleviate DSS-induced colon colitis in mice by modulating NF-κB, MAPK, and microbiota composition.
32701080	5	43	theme	increased	813:821	arg1	weight					828:833	increased body weight	813:833	increased body weight	813:833	The administration of sturgeon hydrolysates (SH) significantly decreased the severity of DSS-induced damage, evidenced by increased body weight, colon length, and decreased disease activity index (DAI) and histological scores.
32701080	3	44	theme	biological	434:443	arg1	activities					445:454	the biological activities	430:454	the biological activities of sturgeon muscle-derived hydrolysates	430:494	Studying the biological activities of sturgeon muscle-derived hydrolysates holds much promise for adding value to sturgeon.
32701080	5	45	theme	body	823:826	arg1	weight					828:833	increased body weight	813:833	increased body weight	813:833	The administration of sturgeon hydrolysates (SH) significantly decreased the severity of DSS-induced damage, evidenced by increased body weight, colon length, and decreased disease activity index (DAI) and histological scores.
32701080	7	46	from	activation	1088:1097	arg1	colon					1137:1141	the colon	1133:1141	the colon	1133:1141	Western blotting results revealed that SH suppressed DSS-induced activation of the NF-κB and MAPK pathways in the colon.
32701080	1	47	used	utilized	202:209	arg2	production					170:179	caviar production	163:179	caviar production	163:179	Sturgeon muscle byproduct collected after caviar production is usually not fully utilized, and sometimes may be discarded, thus causing a lot of waste.
32701080	3	48	theme	hydrolysates	483:494	arg1	activities					445:454	the biological activities	430:454	the biological activities of sturgeon muscle-derived hydrolysates	430:494	Studying the biological activities of sturgeon muscle-derived hydrolysates holds much promise for adding value to sturgeon.
32701080	8	49	theme	microbiota	1205:1214	arg1	alteration					1183:1192	the alteration	1179:1192	the alteration of the gut microbiota in colitic mice	1179:1230	Furthermore, SH partially restored the alteration of the gut microbiota in colitic mice.
32701080	3	50	link	muscle-derived	468:481	arg1	hydrolysates					483:494	sturgeon muscle-derived hydrolysates	459:494	sturgeon muscle-derived hydrolysates	459:494	Studying the biological activities of sturgeon muscle-derived hydrolysates holds much promise for adding value to sturgeon.
32701080	7	51	from	pathways	1121:1128	arg1	colon					1137:1141	the colon	1133:1141	the colon	1133:1141	Western blotting results revealed that SH suppressed DSS-induced activation of the NF-κB and MAPK pathways in the colon.
32701080	5	52	theme	sturgeon	713:720	arg1	SH					736:737	SH	736:737	SH	736:737	The administration of sturgeon hydrolysates (SH) significantly decreased the severity of DSS-induced damage, evidenced by increased body weight, colon length, and decreased disease activity index (DAI) and histological scores.
32701080	5	52	theme	sturgeon	713:720	arg1	hydrolysates					722:733	sturgeon hydrolysates	713:733	sturgeon hydrolysates (SH)	713:738	The administration of sturgeon hydrolysates (SH) significantly decreased the severity of DSS-induced damage, evidenced by increased body weight, colon length, and decreased disease activity index (DAI) and histological scores.
32701080	2	53	contain	have	372:375	arg2	activities					409:418	versatile beneficial biological activities	377:418	versatile beneficial biological activities	377:418	Yet dietary protein hydrolysates, which may be derived from sturgeon muscle, have been reported to have versatile beneficial biological activities.
32701080	2	53	contain	have	372:375	arg1	hydrolysates					293:304	dietary protein hydrolysates	277:304	dietary protein hydrolysates	277:304	Yet dietary protein hydrolysates, which may be derived from sturgeon muscle, have been reported to have versatile beneficial biological activities.
32701080	9	54	theme	Bacteroidetes	1352:1364	arg1	S24-7					1366:1370	Bacteroidetes S24-7	1352:1370	Bacteroidetes S24-7	1352:1370	SH increased the Bacteroidetes/Firmicutes ratio and the relative abundance of Ruminococcaceae, Porphyromonadaceae, and Bacteroidetes S24-7, while decreased the abundance of potentially harmful bacteria Erysipelotrichaceae and Enterococcaceae.
32701080	3	55	theme	sturgeon	459:466	arg1	hydrolysates					483:494	sturgeon muscle-derived hydrolysates	459:494	sturgeon muscle-derived hydrolysates	459:494	Studying the biological activities of sturgeon muscle-derived hydrolysates holds much promise for adding value to sturgeon.
32701080	6	56	theme	TNF-α	1016:1020	arg1	levels					989:994	the serum levels	979:994	the serum levels of IL-6, IL-1β, and TNF-α	979:1020	SH also inhibited myeloperoxidase (MPO) activity and reduced the serum levels of IL-6, IL-1β, and TNF-α.
32701080	5	57	theme	hydrolysates	722:733	arg1	administration					695:708	The administration	691:708	The administration of sturgeon hydrolysates (SH)	691:738	The administration of sturgeon hydrolysates (SH) significantly decreased the severity of DSS-induced damage, evidenced by increased body weight, colon length, and decreased disease activity index (DAI) and histological scores.
32701080	9	58	theme	Bacteroidetes/Firmicutes	1250:1273	arg1	ratio					1275:1279	the Bacteroidetes/Firmicutes ratio	1246:1279	the Bacteroidetes/Firmicutes ratio	1246:1279	SH increased the Bacteroidetes/Firmicutes ratio and the relative abundance of Ruminococcaceae, Porphyromonadaceae, and Bacteroidetes S24-7, while decreased the abundance of potentially harmful bacteria Erysipelotrichaceae and Enterococcaceae.
32701080	10	59	theme	DSS-induced	1516:1526	arg1	colitis					1528:1534	DSS-induced colitis	1516:1534	DSS-induced colitis	1516:1534	These results suggest that SH inhibited DSS-induced colitis by regulating the NF-κB and MAPK pathways and modulating microbiota composition.
32701080	1	60	theme	muscle	130:135	arg1	byproduct					137:145	Sturgeon muscle byproduct	121:145	Sturgeon muscle byproduct	121:145	Sturgeon muscle byproduct collected after caviar production is usually not fully utilized, and sometimes may be discarded, thus causing a lot of waste.
32701080	1	61	theme	Sturgeon	121:128	arg1	byproduct					137:145	Sturgeon muscle byproduct	121:145	Sturgeon muscle byproduct	121:145	Sturgeon muscle byproduct collected after caviar production is usually not fully utilized, and sometimes may be discarded, thus causing a lot of waste.
32701080	5	62	theme	DSS-induced	780:790	arg1	damage					792:797	DSS-induced damage	780:797	DSS-induced damage	780:797	The administration of sturgeon hydrolysates (SH) significantly decreased the severity of DSS-induced damage, evidenced by increased body weight, colon length, and decreased disease activity index (DAI) and histological scores.
32701080	5	63	theme	activity	872:879	arg1	DAI					888:890	DAI	888:890	DAI	888:890	The administration of sturgeon hydrolysates (SH) significantly decreased the severity of DSS-induced damage, evidenced by increased body weight, colon length, and decreased disease activity index (DAI) and histological scores.
32701080	5	63	theme	activity	872:879	arg1	index					881:885	decreased disease activity index	854:885	decreased disease activity index (DAI)	854:891	The administration of sturgeon hydrolysates (SH) significantly decreased the severity of DSS-induced damage, evidenced by increased body weight, colon length, and decreased disease activity index (DAI) and histological scores.
32701080	4	64	theme	hydrolysates	647:658	arg1	effects					612:618	the therapeutic anti-inflammatory effects	578:618	the therapeutic anti-inflammatory effects of sturgeon muscle-derived hydrolysates	578:658	The current study aimed to study the therapeutic anti-inflammatory effects of sturgeon muscle-derived hydrolysates and the underlying mechanisms.
32701080	4	64	theme	hydrolysates	647:658	arg1	mechanisms					679:688	the underlying mechanisms	664:688	the underlying mechanisms	664:688	The current study aimed to study the therapeutic anti-inflammatory effects of sturgeon muscle-derived hydrolysates and the underlying mechanisms.
32701080	8	65	theme	colitic	1219:1225	arg1	mice					1227:1230	colitic mice	1219:1230	colitic mice	1219:1230	Furthermore, SH partially restored the alteration of the gut microbiota in colitic mice.
32701080	4	66	theme	sturgeon	623:630	arg1	hydrolysates					647:658	sturgeon muscle-derived hydrolysates	623:658	sturgeon muscle-derived hydrolysates	623:658	The current study aimed to study the therapeutic anti-inflammatory effects of sturgeon muscle-derived hydrolysates and the underlying mechanisms.
32701080	5	67	theme	damage	792:797	arg1	severity					768:775	the severity	764:775	the severity of DSS-induced damage	764:797	The administration of sturgeon hydrolysates (SH) significantly decreased the severity of DSS-induced damage, evidenced by increased body weight, colon length, and decreased disease activity index (DAI) and histological scores.
32701080	6	68	theme	myeloperoxidase	936:950	arg1	activity					958:965	myeloperoxidase (MPO) activity	936:965	myeloperoxidase (MPO) activity	936:965	SH also inhibited myeloperoxidase (MPO) activity and reduced the serum levels of IL-6, IL-1β, and TNF-α.
32701080	4	69	link	muscle-derived	632:645	arg1	hydrolysates					647:658	sturgeon muscle-derived hydrolysates	623:658	sturgeon muscle-derived hydrolysates	623:658	The current study aimed to study the therapeutic anti-inflammatory effects of sturgeon muscle-derived hydrolysates and the underlying mechanisms.
32701080	2	70	theme	sturgeon	333:340	arg1	muscle					342:347	sturgeon muscle	333:347	sturgeon muscle	333:347	Yet dietary protein hydrolysates, which may be derived from sturgeon muscle, have been reported to have versatile beneficial biological activities.
32701080	6	71	theme	IL-1β	1005:1009	arg1	levels					989:994	the serum levels	979:994	the serum levels of IL-6, IL-1β, and TNF-α	979:1020	SH also inhibited myeloperoxidase (MPO) activity and reduced the serum levels of IL-6, IL-1β, and TNF-α.
34163471	7	0	from	effect	1236:1241	arg1	secretion					1278:1286	cytokine secretion	1269:1286	cytokine secretion in monocyte cultures	1269:1307	To further explore whether chronic inflammation in ESRD patients could be more related to LPS composition rather than its quantity, we tested the effect of HV and patient sera on cytokine secretion in monocyte cultures.
34163471	7	1	theme	chronic	1117:1123	arg1	inflammation					1125:1136	chronic inflammation	1117:1136	chronic inflammation in ESRD patients	1117:1153	To further explore whether chronic inflammation in ESRD patients could be more related to LPS composition rather than its quantity, we tested the effect of HV and patient sera on cytokine secretion in monocyte cultures.
34163471	1	2	theme	gut	291:293	arg1	translocation					305:317	gut bacterial translocation	291:317	gut bacterial translocation	291:317	Chronic kidney disease induces disruption of the intestinal epithelial barrier, leading to gut bacterial translocation.
34163471	6	3	from	cytokines	1036:1044	arg1	patients					1054:1061	ESRD patients	1049:1061	ESRD patients	1049:1061	Higher serum concentrations of soluble CD14 and pro-inflammatory cytokines in ESRD patients confirmed this hypothesis.
34163471	7	4	theme	LPS	1180:1182	arg1	composition					1184:1194	LPS composition	1180:1194	LPS composition rather than its quantity	1180:1219	To further explore whether chronic inflammation in ESRD patients could be more related to LPS composition rather than its quantity, we tested the effect of HV and patient sera on cytokine secretion in monocyte cultures.
34163471	5	5	theme	higher	839:844	arg1	concentration					846:858	higher concentration	839:858	higher concentration of high-density lipoproteins	839:887	Lower LPS activity to mass ratio and higher concentration of high-density lipoproteins were found in HV, suggesting a better plasma capacity to neutralize LPS activity.
34163471	10	6	from	capacity	1741:1748	arg1	subtypes					1786:1793	translocated LPS subtypes	1769:1793	translocated LPS subtypes	1769:1793	Thereby, septic inflammation observed in ESRD patients may be not related to higher bacterial translocation, but to reduced LPS neutralization capacity and differences in translocated LPS subtypes.
34163471	3	7	theme	end-stage	568:576	arg1	ESRD					593:596	ESRD	593:596	ESRD	593:596	This was done in end-stage renal disease (ESRD) patients and healthy volunteers (HV).
34163471	3	7	theme	end-stage	568:576	arg1	disease					584:590	end-stage renal disease	568:590	end-stage renal disease (ESRD) patients	568:606	This was done in end-stage renal disease (ESRD) patients and healthy volunteers (HV).
34163471	1	8	theme	kidney	208:213	arg1	disease					215:221	Chronic kidney disease	200:221	Chronic kidney disease	200:221	Chronic kidney disease induces disruption of the intestinal epithelial barrier, leading to gut bacterial translocation.
34163471	10	9	from	differences	1754:1764	arg1	subtypes					1786:1793	translocated LPS subtypes	1769:1793	translocated LPS subtypes	1769:1793	Thereby, septic inflammation observed in ESRD patients may be not related to higher bacterial translocation, but to reduced LPS neutralization capacity and differences in translocated LPS subtypes.
34163471	10	10	theme	septic	1607:1612	arg1	inflammation					1614:1625	septic inflammation	1607:1625	septic inflammation observed in ESRD patients	1607:1651	Thereby, septic inflammation observed in ESRD patients may be not related to higher bacterial translocation, but to reduced LPS neutralization capacity and differences in translocated LPS subtypes.
34163471	5	11	theme	Lower	802:806	arg1	activity					812:819	Lower LPS activity	802:819	Lower LPS activity to mass ratio	802:833	Lower LPS activity to mass ratio and higher concentration of high-density lipoproteins were found in HV, suggesting a better plasma capacity to neutralize LPS activity.
34163471	8	12	theme	chain	1461:1465	arg1	A-LPS					1473:1477	18-carbon chain lipid A-LPS	1451:1477	18-carbon chain lipid A-LPS	1451:1477	Sera with predominance of 14-carbon chain lipid A-LPS induced higher secretion of pro-inflammatory cytokines than those with predominance of 18-carbon chain lipid A-LPS.
34163471	10	13	theme	ESRD	1639:1642	arg1	patients					1644:1651	ESRD patients	1639:1651	ESRD patients	1639:1651	Thereby, septic inflammation observed in ESRD patients may be not related to higher bacterial translocation, but to reduced LPS neutralization capacity and differences in translocated LPS subtypes.
34163471	3	14	theme	disease	584:590	arg1	patients					599:606	end-stage renal disease (ESRD) patients	568:606	end-stage renal disease (ESRD) patients	568:606	This was done in end-stage renal disease (ESRD) patients and healthy volunteers (HV).
34163471	8	15	theme	A-LPS	1473:1477	arg1	predominance					1435:1446	predominance	1435:1446	predominance of 18-carbon chain lipid A-LPS	1435:1477	Sera with predominance of 14-carbon chain lipid A-LPS induced higher secretion of pro-inflammatory cytokines than those with predominance of 18-carbon chain lipid A-LPS.
34163471	4	16	theme	significant	705:715	arg1	differences					717:727	significant differences	705:727	significant differences in composition of translocated LPS based on lipid A carbon chain length	705:799	We observed both higher LPS concentration in healthy volunteers and significant differences in composition of translocated LPS based on lipid A carbon chain length.
34163471	0	17	theme	Monocyte	168:175	arg1	Response					190:197	Monocyte Inflammatory Response	168:197	Monocyte Inflammatory Response	168:197	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.
34163471	3	18	theme	healthy	612:618	arg1	HV					632:633	HV	632:633	HV	632:633	This was done in end-stage renal disease (ESRD) patients and healthy volunteers (HV).
34163471	3	18	theme	healthy	612:618	arg1	volunteers					620:629	healthy volunteers	612:629	healthy volunteers (HV)	612:634	This was done in end-stage renal disease (ESRD) patients and healthy volunteers (HV).
34163471	9	19	theme	LPS-induced	1514:1524	arg1	production					1535:1544	LPS-induced cytokine production	1514:1544	LPS-induced cytokine production by monocytes	1514:1557	TLR4 or LPS antagonists decreased LPS-induced cytokine production by monocytes, demonstrating an LPS-specific effect.
34163471	6	20	theme	CD14	1010:1013	arg1	concentrations					984:997	Higher serum concentrations	971:997	Higher serum concentrations of soluble CD14 and pro-inflammatory cytokines in ESRD patients	971:1061	Higher serum concentrations of soluble CD14 and pro-inflammatory cytokines in ESRD patients confirmed this hypothesis.
34163471	4	21	theme	LPS	661:663	arg1	concentration					665:677	higher LPS concentration	654:677	higher LPS concentration in healthy volunteers	654:699	We observed both higher LPS concentration in healthy volunteers and significant differences in composition of translocated LPS based on lipid A carbon chain length.
34163471	0	22	theme	Individual	52:61	arg1	Translocation					77:89	Healthy Individual Gut Bacterial Translocation	44:89	Healthy Individual Gut Bacterial Translocation	44:89	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.
34163471	5	23	theme	lipoproteins	876:887	arg1	activity					812:819	Lower LPS activity	802:819	Lower LPS activity to mass ratio	802:833	Lower LPS activity to mass ratio and higher concentration of high-density lipoproteins were found in HV, suggesting a better plasma capacity to neutralize LPS activity.
34163471	5	23	theme	lipoproteins	876:887	arg1	concentration					846:858	higher concentration	839:858	higher concentration of high-density lipoproteins	839:887	Lower LPS activity to mass ratio and higher concentration of high-density lipoproteins were found in HV, suggesting a better plasma capacity to neutralize LPS activity.
34163471	0	24	theme	Bacterial	67:75	arg1	Translocation					77:89	Healthy Individual Gut Bacterial Translocation	44:89	Healthy Individual Gut Bacterial Translocation	44:89	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.
34163471	6	25	theme	serum	978:982	arg1	concentrations					984:997	Higher serum concentrations	971:997	Higher serum concentrations of soluble CD14 and pro-inflammatory cytokines in ESRD patients	971:1061	Higher serum concentrations of soluble CD14 and pro-inflammatory cytokines in ESRD patients confirmed this hypothesis.
34163471	2	26	theme	carbon	530:535	arg1	length					543:548	lipid A carbon chain length	522:548	total LPS as well as LPS lipid A carbon chain length	497:548	Here, we appreciated bacterial translocation by analyzing circulating lipopolysaccharides (LPS) using two methods, one measuring only active free LPS, and the other quantifying total LPS as well as LPS lipid A carbon chain length.
34163471	4	27	theme	LPS	760:762	arg1	composition					732:742	composition	732:742	composition of translocated LPS based on lipid A carbon chain length	732:799	We observed both higher LPS concentration in healthy volunteers and significant differences in composition of translocated LPS based on lipid A carbon chain length.
34163471	2	28	theme	lipid	522:526	arg1	length					543:548	lipid A carbon chain length	522:548	total LPS as well as LPS lipid A carbon chain length	497:548	Here, we appreciated bacterial translocation by analyzing circulating lipopolysaccharides (LPS) using two methods, one measuring only active free LPS, and the other quantifying total LPS as well as LPS lipid A carbon chain length.
34163471	8	29	theme	chain	1346:1350	arg1	A-LPS					1358:1362	14-carbon chain lipid A-LPS	1336:1362	14-carbon chain lipid A-LPS	1336:1362	Sera with predominance of 14-carbon chain lipid A-LPS induced higher secretion of pro-inflammatory cytokines than those with predominance of 18-carbon chain lipid A-LPS.
34163471	10	30	theme	higher	1675:1680	arg1	translocation					1692:1704	higher bacterial translocation	1675:1704	higher bacterial translocation	1675:1704	Thereby, septic inflammation observed in ESRD patients may be not related to higher bacterial translocation, but to reduced LPS neutralization capacity and differences in translocated LPS subtypes.
34163471	0	31	theme	Carbon	102:107	arg1	Insights					4:11	New Insights	0:11	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.	0:198	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.
34163471	0	31	theme	Carbon	102:107	arg1	Composition					109:119	Different Carbon Composition	92:119	Different Carbon Composition of Lipopolysaccharides	92:142	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.
34163471	5	32	located	found	894:898	arg1	HV					903:904	HV	903:904	HV	903:904	Lower LPS activity to mass ratio and higher concentration of high-density lipoproteins were found in HV, suggesting a better plasma capacity to neutralize LPS activity.
34163471	5	32	located	found	894:898	arg2	concentration					846:858	higher concentration	839:858	higher concentration of high-density lipoproteins	839:887	Lower LPS activity to mass ratio and higher concentration of high-density lipoproteins were found in HV, suggesting a better plasma capacity to neutralize LPS activity.
34163471	5	32	located	found	894:898	arg2	activity					812:819	Lower LPS activity	802:819	Lower LPS activity to mass ratio	802:833	Lower LPS activity to mass ratio and higher concentration of high-density lipoproteins were found in HV, suggesting a better plasma capacity to neutralize LPS activity.
34163471	9	33	theme	LPS-specific	1577:1588	arg1	effect					1590:1595	an LPS-specific effect	1574:1595	an LPS-specific effect	1574:1595	TLR4 or LPS antagonists decreased LPS-induced cytokine production by monocytes, demonstrating an LPS-specific effect.
34163471	6	34	from	concentrations	984:997	arg1	patients					1054:1061	ESRD patients	1049:1061	ESRD patients	1049:1061	Higher serum concentrations of soluble CD14 and pro-inflammatory cytokines in ESRD patients confirmed this hypothesis.
34163471	8	35	theme	A-LPS	1358:1362	arg1	predominance					1320:1331	predominance	1320:1331	predominance of 14-carbon chain lipid A-LPS	1320:1362	Sera with predominance of 14-carbon chain lipid A-LPS induced higher secretion of pro-inflammatory cytokines than those with predominance of 18-carbon chain lipid A-LPS.
34163471	0	36	theme	New	0:2	arg1	Insights					4:11	New Insights	0:11	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.	0:198	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.
34163471	0	36	theme	New	0:2	arg1	Impact					158:163	Different Impact	148:163	Different Impact on Monocyte Inflammatory Response	148:197	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.
34163471	0	36	theme	New	0:2	arg1	Composition					109:119	Different Carbon Composition	92:119	Different Carbon Composition of Lipopolysaccharides	92:142	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.
34163471	1	37	theme	bacterial	295:303	arg1	translocation					305:317	gut bacterial translocation	291:317	gut bacterial translocation	291:317	Chronic kidney disease induces disruption of the intestinal epithelial barrier, leading to gut bacterial translocation.
34163471	5	38	theme	better	920:925	arg1	capacity					934:941	a better plasma capacity	918:941	a better plasma capacity to neutralize LPS activity	918:968	Lower LPS activity to mass ratio and higher concentration of high-density lipoproteins were found in HV, suggesting a better plasma capacity to neutralize LPS activity.
34163471	8	39	theme	higher	1372:1377	arg1	secretion					1379:1387	higher secretion	1372:1387	higher secretion of pro-inflammatory cytokines	1372:1417	Sera with predominance of 14-carbon chain lipid A-LPS induced higher secretion of pro-inflammatory cytokines than those with predominance of 18-carbon chain lipid A-LPS.
34163471	2	40	theme	active	454:459	arg1	LPS					466:468	only active free LPS	449:468	only active free LPS	449:468	Here, we appreciated bacterial translocation by analyzing circulating lipopolysaccharides (LPS) using two methods, one measuring only active free LPS, and the other quantifying total LPS as well as LPS lipid A carbon chain length.
34163471	5	41	theme	LPS	808:810	arg1	activity					812:819	Lower LPS activity	802:819	Lower LPS activity to mass ratio	802:833	Lower LPS activity to mass ratio and higher concentration of high-density lipoproteins were found in HV, suggesting a better plasma capacity to neutralize LPS activity.
34163471	2	42	dep	LPS	518:520	arg1	length					543:548	lipid A carbon chain length	522:548	total LPS as well as LPS lipid A carbon chain length	497:548	Here, we appreciated bacterial translocation by analyzing circulating lipopolysaccharides (LPS) using two methods, one measuring only active free LPS, and the other quantifying total LPS as well as LPS lipid A carbon chain length.
34163471	7	43	theme	monocyte	1291:1298	arg1	cultures					1300:1307	monocyte cultures	1291:1307	monocyte cultures	1291:1307	To further explore whether chronic inflammation in ESRD patients could be more related to LPS composition rather than its quantity, we tested the effect of HV and patient sera on cytokine secretion in monocyte cultures.
34163471	2	44	theme	total	497:501	arg1	LPS					503:505	total LPS	497:505	total LPS as well as LPS lipid A carbon chain length	497:548	Here, we appreciated bacterial translocation by analyzing circulating lipopolysaccharides (LPS) using two methods, one measuring only active free LPS, and the other quantifying total LPS as well as LPS lipid A carbon chain length.
34163471	6	45	theme	cytokines	1036:1044	arg1	concentrations					984:997	Higher serum concentrations	971:997	Higher serum concentrations of soluble CD14 and pro-inflammatory cytokines in ESRD patients	971:1061	Higher serum concentrations of soluble CD14 and pro-inflammatory cytokines in ESRD patients confirmed this hypothesis.
34163471	0	46	theme	Renal	26:30	arg1	Disease					32:38	End-Stage Renal Disease	16:38	End-Stage Renal Disease	16:38	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.
34163471	6	47	theme	pro-inflammatory	1019:1034	arg1	cytokines					1036:1044	pro-inflammatory cytokines	1019:1044	pro-inflammatory cytokines	1019:1044	Higher serum concentrations of soluble CD14 and pro-inflammatory cytokines in ESRD patients confirmed this hypothesis.
34163471	8	48	theme	cytokines	1409:1417	arg1	secretion					1379:1387	higher secretion	1372:1387	higher secretion of pro-inflammatory cytokines	1372:1417	Sera with predominance of 14-carbon chain lipid A-LPS induced higher secretion of pro-inflammatory cytokines than those with predominance of 18-carbon chain lipid A-LPS.
34163471	2	49	theme	circulating	378:388	arg1	LPS					411:413	LPS	411:413	LPS	411:413	Here, we appreciated bacterial translocation by analyzing circulating lipopolysaccharides (LPS) using two methods, one measuring only active free LPS, and the other quantifying total LPS as well as LPS lipid A carbon chain length.
34163471	2	49	theme	circulating	378:388	arg1	lipopolysaccharides					390:408	circulating lipopolysaccharides	378:408	circulating lipopolysaccharides (LPS) using two methods, one measuring only active free LPS, and the other quantifying total LPS as well as LPS lipid A carbon chain length	378:548	Here, we appreciated bacterial translocation by analyzing circulating lipopolysaccharides (LPS) using two methods, one measuring only active free LPS, and the other quantifying total LPS as well as LPS lipid A carbon chain length.
34163471	8	50	with	Sera	1310:1313	arg1	predominance					1320:1331	predominance	1320:1331	predominance of 14-carbon chain lipid A-LPS	1320:1362	Sera with predominance of 14-carbon chain lipid A-LPS induced higher secretion of pro-inflammatory cytokines than those with predominance of 18-carbon chain lipid A-LPS.
34163471	4	51	theme	A	779:779	arg1	length					794:799	lipid A carbon chain length	773:799	lipid A carbon chain length	773:799	We observed both higher LPS concentration in healthy volunteers and significant differences in composition of translocated LPS based on lipid A carbon chain length.
34163471	10	52	theme	reduced	1714:1720	arg1	capacity					1741:1748	reduced LPS neutralization capacity	1714:1748	reduced LPS neutralization capacity	1714:1748	Thereby, septic inflammation observed in ESRD patients may be not related to higher bacterial translocation, but to reduced LPS neutralization capacity and differences in translocated LPS subtypes.
34163471	10	53	theme	translocated	1769:1780	arg1	subtypes					1786:1793	translocated LPS subtypes	1769:1793	translocated LPS subtypes	1769:1793	Thereby, septic inflammation observed in ESRD patients may be not related to higher bacterial translocation, but to reduced LPS neutralization capacity and differences in translocated LPS subtypes.
34163471	7	54	theme	patient	1253:1259	arg1	sera					1261:1264	HV and patient sera	1246:1264	sera	1261:1264	To further explore whether chronic inflammation in ESRD patients could be more related to LPS composition rather than its quantity, we tested the effect of HV and patient sera on cytokine secretion in monocyte cultures.
34163471	4	55	theme	chain	788:792	arg1	length					794:799	lipid A carbon chain length	773:799	lipid A carbon chain length	773:799	We observed both higher LPS concentration in healthy volunteers and significant differences in composition of translocated LPS based on lipid A carbon chain length.
34163471	1	56	theme	epithelial	260:269	arg1	barrier					271:277	the intestinal epithelial barrier	245:277	the intestinal epithelial barrier	245:277	Chronic kidney disease induces disruption of the intestinal epithelial barrier, leading to gut bacterial translocation.
34163471	0	57	theme	Healthy	44:50	arg1	Translocation					77:89	Healthy Individual Gut Bacterial Translocation	44:89	Healthy Individual Gut Bacterial Translocation	44:89	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.
34163471	7	58	theme	HV	1246:1247	arg1	sera					1261:1264	HV and patient sera	1246:1264	sera	1261:1264	To further explore whether chronic inflammation in ESRD patients could be more related to LPS composition rather than its quantity, we tested the effect of HV and patient sera on cytokine secretion in monocyte cultures.
34163471	4	59	from	concentration	665:677	arg1	composition					732:742	composition	732:742	composition of translocated LPS based on lipid A carbon chain length	732:799	We observed both higher LPS concentration in healthy volunteers and significant differences in composition of translocated LPS based on lipid A carbon chain length.
34163471	4	59	from	concentration	665:677	arg1	volunteers					690:699	healthy volunteers	682:699	healthy volunteers	682:699	We observed both higher LPS concentration in healthy volunteers and significant differences in composition of translocated LPS based on lipid A carbon chain length.
34163471	6	60	from	patients	1054:1061	arg1	concentrations					984:997	Higher serum concentrations	971:997	Higher serum concentrations of soluble CD14 and pro-inflammatory cytokines in ESRD patients	971:1061	Higher serum concentrations of soluble CD14 and pro-inflammatory cytokines in ESRD patients confirmed this hypothesis.
34163471	0	61	from	Impact	158:163	arg1	Response					190:197	Monocyte Inflammatory Response	168:197	Monocyte Inflammatory Response	168:197	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.
34163471	10	62	theme	neutralization	1726:1739	arg1	capacity					1741:1748	reduced LPS neutralization capacity	1714:1748	reduced LPS neutralization capacity	1714:1748	Thereby, septic inflammation observed in ESRD patients may be not related to higher bacterial translocation, but to reduced LPS neutralization capacity and differences in translocated LPS subtypes.
34163471	10	63	theme	LPS	1782:1784	arg1	subtypes					1786:1793	translocated LPS subtypes	1769:1793	translocated LPS subtypes	1769:1793	Thereby, septic inflammation observed in ESRD patients may be not related to higher bacterial translocation, but to reduced LPS neutralization capacity and differences in translocated LPS subtypes.
34163471	2	64	theme	bacterial	341:349	arg1	translocation					351:363	bacterial translocation	341:363	bacterial translocation	341:363	Here, we appreciated bacterial translocation by analyzing circulating lipopolysaccharides (LPS) using two methods, one measuring only active free LPS, and the other quantifying total LPS as well as LPS lipid A carbon chain length.
34163471	1	65	theme	Chronic	200:206	arg1	disease					215:221	Chronic kidney disease	200:221	Chronic kidney disease	200:221	Chronic kidney disease induces disruption of the intestinal epithelial barrier, leading to gut bacterial translocation.
34163471	0	66	theme	Lipopolysaccharides	124:142	arg1	Insights					4:11	New Insights	0:11	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.	0:198	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.
34163471	0	66	theme	Lipopolysaccharides	124:142	arg1	Impact					158:163	Different Impact	148:163	Different Impact on Monocyte Inflammatory Response	148:197	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.
34163471	0	66	theme	Lipopolysaccharides	124:142	arg1	Composition					109:119	Different Carbon Composition	92:119	Different Carbon Composition of Lipopolysaccharides	92:142	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.
34163471	8	67	theme	18-carbon	1451:1459	arg1	A-LPS					1473:1477	18-carbon chain lipid A-LPS	1451:1477	18-carbon chain lipid A-LPS	1451:1477	Sera with predominance of 14-carbon chain lipid A-LPS induced higher secretion of pro-inflammatory cytokines than those with predominance of 18-carbon chain lipid A-LPS.
34163471	0	68	theme	Different	148:156	arg1	Insights					4:11	New Insights	0:11	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.	0:198	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.
34163471	0	68	theme	Different	148:156	arg1	Impact					158:163	Different Impact	148:163	Different Impact on Monocyte Inflammatory Response	148:197	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.
34163471	5	69	theme	mass	824:827	arg1	ratio					829:833	mass ratio	824:833	mass ratio	824:833	Lower LPS activity to mass ratio and higher concentration of high-density lipoproteins were found in HV, suggesting a better plasma capacity to neutralize LPS activity.
34163471	2	70	theme	chain	537:541	arg1	length					543:548	lipid A carbon chain length	522:548	total LPS as well as LPS lipid A carbon chain length	497:548	Here, we appreciated bacterial translocation by analyzing circulating lipopolysaccharides (LPS) using two methods, one measuring only active free LPS, and the other quantifying total LPS as well as LPS lipid A carbon chain length.
34163471	8	71	theme	lipid	1467:1471	arg1	A-LPS					1473:1477	18-carbon chain lipid A-LPS	1451:1477	18-carbon chain lipid A-LPS	1451:1477	Sera with predominance of 14-carbon chain lipid A-LPS induced higher secretion of pro-inflammatory cytokines than those with predominance of 18-carbon chain lipid A-LPS.
34163471	3	72	theme	renal	578:582	arg1	ESRD					593:596	ESRD	593:596	ESRD	593:596	This was done in end-stage renal disease (ESRD) patients and healthy volunteers (HV).
34163471	3	72	theme	renal	578:582	arg1	disease					584:590	end-stage renal disease	568:590	end-stage renal disease (ESRD) patients	568:606	This was done in end-stage renal disease (ESRD) patients and healthy volunteers (HV).
34163471	9	73	theme	LPS	1488:1490	arg1	antagonists					1492:1502	TLR4 or LPS antagonists	1480:1502	TLR4 or LPS antagonists	1480:1502	TLR4 or LPS antagonists decreased LPS-induced cytokine production by monocytes, demonstrating an LPS-specific effect.
34163471	0	74	from	Composition	109:119	arg1	Response					190:197	Monocyte Inflammatory Response	168:197	Monocyte Inflammatory Response	168:197	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.
34163471	4	75	theme	healthy	682:688	arg1	volunteers					690:699	healthy volunteers	682:699	healthy volunteers	682:699	We observed both higher LPS concentration in healthy volunteers and significant differences in composition of translocated LPS based on lipid A carbon chain length.
34163471	0	76	theme	Inflammatory	177:188	arg1	Response					190:197	Monocyte Inflammatory Response	168:197	Monocyte Inflammatory Response	168:197	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.
34163471	10	77	located	observed	1627:1634	arg1	patients					1644:1651	ESRD patients	1639:1651	ESRD patients	1639:1651	Thereby, septic inflammation observed in ESRD patients may be not related to higher bacterial translocation, but to reduced LPS neutralization capacity and differences in translocated LPS subtypes.
34163471	10	77	located	observed	1627:1634	arg2	inflammation					1614:1625	septic inflammation	1607:1625	septic inflammation observed in ESRD patients	1607:1651	Thereby, septic inflammation observed in ESRD patients may be not related to higher bacterial translocation, but to reduced LPS neutralization capacity and differences in translocated LPS subtypes.
34163471	0	78	theme	Gut	63:65	arg1	Translocation					77:89	Healthy Individual Gut Bacterial Translocation	44:89	Healthy Individual Gut Bacterial Translocation	44:89	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.
34163471	9	79	theme	cytokine	1526:1533	arg1	production					1535:1544	LPS-induced cytokine production	1514:1544	LPS-induced cytokine production by monocytes	1514:1557	TLR4 or LPS antagonists decreased LPS-induced cytokine production by monocytes, demonstrating an LPS-specific effect.
34163471	5	80	theme	high-density	863:874	arg1	lipoproteins					876:887	high-density lipoproteins	863:887	high-density lipoproteins	863:887	Lower LPS activity to mass ratio and higher concentration of high-density lipoproteins were found in HV, suggesting a better plasma capacity to neutralize LPS activity.
34163471	6	81	theme	soluble	1002:1008	arg1	CD14					1010:1013	soluble CD14	1002:1013	soluble CD14	1002:1013	Higher serum concentrations of soluble CD14 and pro-inflammatory cytokines in ESRD patients confirmed this hypothesis.
34163471	4	82	theme	higher	654:659	arg1	concentration					665:677	higher LPS concentration	654:677	higher LPS concentration in healthy volunteers	654:699	We observed both higher LPS concentration in healthy volunteers and significant differences in composition of translocated LPS based on lipid A carbon chain length.
34163471	0	83	from	Insights	4:11	arg1	Disease					32:38	End-Stage Renal Disease	16:38	End-Stage Renal Disease	16:38	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.
34163471	0	83	from	Insights	4:11	arg1	Translocation					77:89	Healthy Individual Gut Bacterial Translocation	44:89	Healthy Individual Gut Bacterial Translocation	44:89	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.
34163471	0	84	theme	Different	92:100	arg1	Insights					4:11	New Insights	0:11	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.	0:198	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.
34163471	0	84	theme	Different	92:100	arg1	Composition					109:119	Different Carbon Composition	92:119	Different Carbon Composition of Lipopolysaccharides	92:142	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.
34163471	6	85	theme	Higher	971:976	arg1	concentrations					984:997	Higher serum concentrations	971:997	Higher serum concentrations of soluble CD14 and pro-inflammatory cytokines in ESRD patients	971:1061	Higher serum concentrations of soluble CD14 and pro-inflammatory cytokines in ESRD patients confirmed this hypothesis.
34163471	2	86	theme	A	528:528	arg1	length					543:548	lipid A carbon chain length	522:548	total LPS as well as LPS lipid A carbon chain length	497:548	Here, we appreciated bacterial translocation by analyzing circulating lipopolysaccharides (LPS) using two methods, one measuring only active free LPS, and the other quantifying total LPS as well as LPS lipid A carbon chain length.
34163471	6	87	from	CD14	1010:1013	arg1	patients					1054:1061	ESRD patients	1049:1061	ESRD patients	1049:1061	Higher serum concentrations of soluble CD14 and pro-inflammatory cytokines in ESRD patients confirmed this hypothesis.
34163471	4	88	theme	translocated	747:758	arg1	LPS					760:762	translocated LPS	747:762	translocated LPS based on lipid A carbon chain length	747:799	We observed both higher LPS concentration in healthy volunteers and significant differences in composition of translocated LPS based on lipid A carbon chain length.
34163471	5	89	theme	LPS	957:959	arg1	activity					961:968	LPS activity	957:968	LPS activity	957:968	Lower LPS activity to mass ratio and higher concentration of high-density lipoproteins were found in HV, suggesting a better plasma capacity to neutralize LPS activity.
34163471	8	90	theme	14-carbon	1336:1344	arg1	A-LPS					1358:1362	14-carbon chain lipid A-LPS	1336:1362	14-carbon chain lipid A-LPS	1336:1362	Sera with predominance of 14-carbon chain lipid A-LPS induced higher secretion of pro-inflammatory cytokines than those with predominance of 18-carbon chain lipid A-LPS.
34163471	8	91	theme	lipid	1352:1356	arg1	A-LPS					1358:1362	14-carbon chain lipid A-LPS	1336:1362	14-carbon chain lipid A-LPS	1336:1362	Sera with predominance of 14-carbon chain lipid A-LPS induced higher secretion of pro-inflammatory cytokines than those with predominance of 18-carbon chain lipid A-LPS.
34163471	0	92	theme	End-Stage	16:24	arg1	Disease					32:38	End-Stage Renal Disease	16:38	End-Stage Renal Disease	16:38	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.
34163471	4	93	from	differences	717:727	arg1	composition					732:742	composition	732:742	composition of translocated LPS based on lipid A carbon chain length	732:799	We observed both higher LPS concentration in healthy volunteers and significant differences in composition of translocated LPS based on lipid A carbon chain length.
34163471	4	93	from	differences	717:727	arg1	volunteers					690:699	healthy volunteers	682:699	healthy volunteers	682:699	We observed both higher LPS concentration in healthy volunteers and significant differences in composition of translocated LPS based on lipid A carbon chain length.
34163471	5	94	theme	plasma	927:932	arg1	capacity					934:941	a better plasma capacity	918:941	a better plasma capacity to neutralize LPS activity	918:968	Lower LPS activity to mass ratio and higher concentration of high-density lipoproteins were found in HV, suggesting a better plasma capacity to neutralize LPS activity.
34163471	6	95	theme	ESRD	1049:1052	arg1	patients					1054:1061	ESRD patients	1049:1061	ESRD patients	1049:1061	Higher serum concentrations of soluble CD14 and pro-inflammatory cytokines in ESRD patients confirmed this hypothesis.
34163471	2	96	theme	free	461:464	arg1	LPS					466:468	only active free LPS	449:468	only active free LPS	449:468	Here, we appreciated bacterial translocation by analyzing circulating lipopolysaccharides (LPS) using two methods, one measuring only active free LPS, and the other quantifying total LPS as well as LPS lipid A carbon chain length.
34163471	9	97	theme	TLR4	1480:1483	arg1	antagonists					1492:1502	TLR4 or LPS antagonists	1480:1502	TLR4 or LPS antagonists	1480:1502	TLR4 or LPS antagonists decreased LPS-induced cytokine production by monocytes, demonstrating an LPS-specific effect.
34163471	7	98	theme	cytokine	1269:1276	arg1	secretion					1278:1286	cytokine secretion	1269:1286	cytokine secretion in monocyte cultures	1269:1307	To further explore whether chronic inflammation in ESRD patients could be more related to LPS composition rather than its quantity, we tested the effect of HV and patient sera on cytokine secretion in monocyte cultures.
34163471	10	99	theme	bacterial	1682:1690	arg1	translocation					1692:1704	higher bacterial translocation	1675:1704	higher bacterial translocation	1675:1704	Thereby, septic inflammation observed in ESRD patients may be not related to higher bacterial translocation, but to reduced LPS neutralization capacity and differences in translocated LPS subtypes.
34163471	7	100	theme	ESRD	1141:1144	arg1	patients					1146:1153	ESRD patients	1141:1153	ESRD patients	1141:1153	To further explore whether chronic inflammation in ESRD patients could be more related to LPS composition rather than its quantity, we tested the effect of HV and patient sera on cytokine secretion in monocyte cultures.
34163471	7	101	from	secretion	1278:1286	arg1	cultures					1300:1307	monocyte cultures	1291:1307	monocyte cultures	1291:1307	To further explore whether chronic inflammation in ESRD patients could be more related to LPS composition rather than its quantity, we tested the effect of HV and patient sera on cytokine secretion in monocyte cultures.
34163471	7	102	theme	sera	1261:1264	arg1	effect					1236:1241	the effect	1232:1241	the effect of HV and patient sera on cytokine secretion in monocyte cultures	1232:1307	To further explore whether chronic inflammation in ESRD patients could be more related to LPS composition rather than its quantity, we tested the effect of HV and patient sera on cytokine secretion in monocyte cultures.
34163471	7	103	from	inflammation	1125:1136	arg1	patients					1146:1153	ESRD patients	1141:1153	ESRD patients	1141:1153	To further explore whether chronic inflammation in ESRD patients could be more related to LPS composition rather than its quantity, we tested the effect of HV and patient sera on cytokine secretion in monocyte cultures.
34163471	8	104	theme	pro-inflammatory	1392:1407	arg1	cytokines					1409:1417	pro-inflammatory cytokines	1392:1417	pro-inflammatory cytokines	1392:1417	Sera with predominance of 14-carbon chain lipid A-LPS induced higher secretion of pro-inflammatory cytokines than those with predominance of 18-carbon chain lipid A-LPS.
34163471	10	105	theme	LPS	1722:1724	arg1	capacity					1741:1748	reduced LPS neutralization capacity	1714:1748	reduced LPS neutralization capacity	1714:1748	Thereby, septic inflammation observed in ESRD patients may be not related to higher bacterial translocation, but to reduced LPS neutralization capacity and differences in translocated LPS subtypes.
34163471	4	106	theme	lipid	773:777	arg1	length					794:799	lipid A carbon chain length	773:799	lipid A carbon chain length	773:799	We observed both higher LPS concentration in healthy volunteers and significant differences in composition of translocated LPS based on lipid A carbon chain length.
34163471	1	107	theme	intestinal	249:258	arg1	barrier					271:277	the intestinal epithelial barrier	245:277	the intestinal epithelial barrier	245:277	Chronic kidney disease induces disruption of the intestinal epithelial barrier, leading to gut bacterial translocation.
34163471	0	108	dep	Insights	4:11	arg1	Insights					4:11	New Insights	0:11	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.	0:198	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.
34163471	0	108	dep	Insights	4:11	arg1	Impact					158:163	Different Impact	148:163	Different Impact on Monocyte Inflammatory Response	148:197	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.
34163471	0	108	dep	Insights	4:11	arg1	Composition					109:119	Different Carbon Composition	92:119	Different Carbon Composition of Lipopolysaccharides	92:142	New Insights on End-Stage Renal Disease and Healthy Individual Gut Bacterial Translocation: Different Carbon Composition of Lipopolysaccharides and Different Impact on Monocyte Inflammatory Response.
34163471	4	109	theme	carbon	781:786	arg1	length					794:799	lipid A carbon chain length	773:799	lipid A carbon chain length	773:799	We observed both higher LPS concentration in healthy volunteers and significant differences in composition of translocated LPS based on lipid A carbon chain length.
34163471	1	110	theme	barrier	271:277	arg1	disruption					231:240	disruption	231:240	disruption of the intestinal epithelial barrier	231:277	Chronic kidney disease induces disruption of the intestinal epithelial barrier, leading to gut bacterial translocation.
33179481	5	0	theme	quantitative	571:582	arg1	evaluation					584:593	a qualitative and quantitative evaluation	553:593	a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour)	553:771	METHODS The aim of the study was to conduct a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour), as well as wheat bran.
33179481	16	1	theme	nutrients	2289:2297	arg1	contents					2277:2284	the contents	2273:2284	the contents of nutrients and natural antioxidants	2273:2322	It was also shown that dehulling of barley and oat grain causes statistically significant differences in the contents of nutrients and natural antioxidants.
33179481	5	2	dep	METHODS	509:515	arg1	was					538:540	was	538:540	was to conduct a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour), as well as wheat bran	538:794	METHODS The aim of the study was to conduct a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour), as well as wheat bran.
33179481	7	3	from	RESULTS	955:961	arg1	materials					979:987	The studied raw materials	963:987	The studied raw materials	963:987	RESULTS The studied raw materials in commercial cereal differ in their chemical composition.
33179481	14	4	theme	saccharides	2050:2060	arg1	exception					1982:1990	the exception	1978:1990	the exception	1978:1990	They are characterized by high contents of tocochromanols, phenolic compounds and water-extractable arabinoxylans, with the exception of white refined wheat flour, which is mainly a source of saccharides.
33179481	14	4	theme	saccharides	2050:2060	arg1	source					2040:2045	a source	2038:2045	a source of saccharides	2038:2060	They are characterized by high contents of tocochromanols, phenolic compounds and water-extractable arabinoxylans, with the exception of white refined wheat flour, which is mainly a source of saccharides.
33179481	2	5	theme	requirements	125:136	arg1	BACKGROUND					103:112	BACKGROUND One	103:116	BACKGROUND One of the requirements for proper nutrition and maintenance of good health	103:188	BACKGROUND One of the requirements for proper nutrition and maintenance of good health is to supply the body, through diet, with an appropriately increased quantity of bioactive compounds.
33179481	4	6	from	bran	439:442	arg1	products					453:460	baked products	447:460	baked products	447:460	The use of such additives as wholegrain flours and bran in baked products provided the basis for research in this paper.
33179481	16	7	theme	natural	2303:2309	arg1	antioxidants					2311:2322	natural antioxidants	2303:2322	natural antioxidants	2303:2322	It was also shown that dehulling of barley and oat grain causes statistically significant differences in the contents of nutrients and natural antioxidants.
33179481	4	8	from	flours	428:433	arg1	products					453:460	baked products	447:460	baked products	447:460	The use of such additives as wholegrain flours and bran in baked products provided the basis for research in this paper.
33179481	13	9	theme	CONCLUSIONS	1776:1786	arg1	products					1794:1801	CONCLUSIONS Wheat products	1776:1801	CONCLUSIONS Wheat products	1776:1801	CONCLUSIONS Wheat products differ in the contents of their individual components.
33179481	9	10	theme	other	1286:1290	arg1	products					1296:1303	other oat products	1286:1303	other oat products	1286:1303	Oat bran, in contrast to other oat products, was characterized by the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch.
33179481	11	11	theme	hydrothermal	1619:1630	arg1	treatment					1632:1640	hydrothermal treatment	1619:1640	hydrothermal treatment	1619:1640	Thermal processes affect the concentration of tocochromanols in the grain, with tocotrienols being more resistant to hydrothermal treatment than tocopherols.
33179481	5	12	theme	qualitative	555:565	arg1	evaluation					584:593	a qualitative and quantitative evaluation	553:593	a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour)	553:771	METHODS The aim of the study was to conduct a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour), as well as wheat bran.
33179481	8	13	theme	grains	1083:1088	arg1	Dehulling					1048:1056	Dehulling	1048:1056	Dehulling of wheat, barley and oat grains	1048:1088	Dehulling of wheat, barley and oat grains significantly contributed to the reduction of minerals, protein and total dietary fiber (TDF) contents, except for the amount of protein in dehulled wheat and oat grains.
33179481	12	14	from	amount	1715:1720	arg1	relation					1743:1750	relation	1743:1750	relation to the original grains	1743:1773	Grain dehulling also significantly decreased the total amount of tocochromanols in relation to the original grains.
33179481	1	15	theme	oat	70:72	arg1	Study					46:50	Study	46:50	Study of wheat, barley, oat and selected grain products.	46:101	Study of wheat, barley, oat and selected grain products.
33179481	5	16	theme	oat	627:629	arg1	grain					631:635	oat grain	627:635	oat grain	627:635	METHODS The aim of the study was to conduct a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour), as well as wheat bran.
33179481	2	17	theme	increased	249:257	arg1	quantity					259:266	an appropriately increased quantity	232:266	an appropriately increased quantity of bioactive compounds	232:289	BACKGROUND One of the requirements for proper nutrition and maintenance of good health is to supply the body, through diet, with an appropriately increased quantity of bioactive compounds.
33179481	5	18	theme	type	735:738	arg1	bran					791:794	wheat bran	785:794	wheat bran	785:794	METHODS The aim of the study was to conduct a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour), as well as wheat bran.
33179481	5	18	theme	type	735:738	arg1	evaluation					584:593	a qualitative and quantitative evaluation	553:593	a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour)	553:771	METHODS The aim of the study was to conduct a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour), as well as wheat bran.
33179481	1	19	theme	products	93:100	arg1	Study					46:50	Study	46:50	Study of wheat, barley, oat and selected grain products.	46:101	Study of wheat, barley, oat and selected grain products.
33179481	10	20	theme	minerals	1455:1462	arg1	content					1444:1450	The lowest content	1433:1450	The lowest content of minerals	1433:1462	The lowest content of minerals was recorded in wheat flour type 550.
33179481	8	21	theme	dietary	1164:1170	arg1	contents					1184:1191	total dietary fiber (TDF) contents	1158:1191	total dietary fiber (TDF) contents	1158:1191	Dehulling of wheat, barley and oat grains significantly contributed to the reduction of minerals, protein and total dietary fiber (TDF) contents, except for the amount of protein in dehulled wheat and oat grains.
33179481	14	22	theme	flour	2015:2019	arg1	exception					1982:1990	the exception	1978:1990	the exception	1978:1990	They are characterized by high contents of tocochromanols, phenolic compounds and water-extractable arabinoxylans, with the exception of white refined wheat flour, which is mainly a source of saccharides.
33179481	14	22	theme	flour	2015:2019	arg1	source					2040:2045	a source	2038:2045	a source of saccharides	2038:2060	They are characterized by high contents of tocochromanols, phenolic compounds and water-extractable arabinoxylans, with the exception of white refined wheat flour, which is mainly a source of saccharides.
33179481	4	23	theme	such	399:402	arg1	additives					404:412	such additives	399:412	such additives	399:412	The use of such additives as wholegrain flours and bran in baked products provided the basis for research in this paper.
33179481	14	24	theme	refined	2001:2007	arg1	flour					2015:2019	white refined wheat flour	1995:2019	white refined wheat flour	1995:2019	They are characterized by high contents of tocochromanols, phenolic compounds and water-extractable arabinoxylans, with the exception of white refined wheat flour, which is mainly a source of saccharides.
33179481	5	25	theme	wheat	609:613	arg1	bran					791:794	wheat bran	785:794	wheat bran	785:794	METHODS The aim of the study was to conduct a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour), as well as wheat bran.
33179481	5	25	theme	wheat	609:613	arg1	evaluation					584:593	a qualitative and quantitative evaluation	553:593	a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour)	553:771	METHODS The aim of the study was to conduct a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour), as well as wheat bran.
33179481	5	26	theme	wheat	785:789	arg1	bran					791:794	wheat bran	785:794	wheat bran	785:794	METHODS The aim of the study was to conduct a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour), as well as wheat bran.
33179481	5	27	theme	barley	616:621	arg1	bran					791:794	wheat bran	785:794	wheat bran	785:794	METHODS The aim of the study was to conduct a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour), as well as wheat bran.
33179481	5	27	theme	barley	616:621	arg1	evaluation					584:593	a qualitative and quantitative evaluation	553:593	a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour)	553:771	METHODS The aim of the study was to conduct a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour), as well as wheat bran.
33179481	5	28	theme	wheat	723:727	arg1	type					735:738	wheat flour type 550	723:742	wheat flour type 550 (all-purpose or plain flour)	723:771	METHODS The aim of the study was to conduct a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour), as well as wheat bran.
33179481	14	29	theme	white	1995:1999	arg1	flour					2015:2019	white refined wheat flour	1995:2019	white refined wheat flour	1995:2019	They are characterized by high contents of tocochromanols, phenolic compounds and water-extractable arabinoxylans, with the exception of white refined wheat flour, which is mainly a source of saccharides.
33179481	0	30	from	Evaluation	0:9	arg1	cereals					37:43	cereals	37:43	cereals	37:43	Evaluation of bioactive compounds in cereals.
33179481	9	31	theme	saccharides	1403:1413	arg1	starch					1425:1430	total starch	1419:1430	total starch	1419:1430	Oat bran, in contrast to other oat products, was characterized by the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch.
33179481	9	31	theme	saccharides	1403:1413	arg1	protein					1361:1367	protein	1361:1367	protein	1361:1367	Oat bran, in contrast to other oat products, was characterized by the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch.
33179481	9	31	theme	saccharides	1403:1413	arg1	amounts					1392:1398	the smallest amounts	1379:1398	the smallest amounts of saccharides and total starch	1379:1430	Oat bran, in contrast to other oat products, was characterized by the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch.
33179481	9	31	theme	saccharides	1403:1413	arg1	saccharides					1403:1413	saccharides	1403:1413	saccharides	1403:1413	Oat bran, in contrast to other oat products, was characterized by the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch.
33179481	9	31	theme	saccharides	1403:1413	arg1	TDF					1370:1372	TDF	1370:1372	TDF	1370:1372	Oat bran, in contrast to other oat products, was characterized by the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch.
33179481	9	31	theme	saccharides	1403:1413	arg1	minerals					1351:1358	minerals	1351:1358	minerals	1351:1358	Oat bran, in contrast to other oat products, was characterized by the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch.
33179481	5	32	theme	study	532:536	arg1	aim					521:523	The aim	517:523	The aim of the study	517:536	METHODS The aim of the study was to conduct a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour), as well as wheat bran.
33179481	1	33	theme	wheat	55:59	arg1	Study					46:50	Study	46:50	Study of wheat, barley, oat and selected grain products.	46:101	Study of wheat, barley, oat and selected grain products.
33179481	6	34	theme	chemical	852:859	arg1	composition					861:871	chemical composition	852:871	chemical composition	852:871	Additionally, analyses were performed to determine the chemical composition and contents of nutrients, selected bioactive compounds and antioxidant activity.
33179481	8	35	theme	protein	1219:1225	arg1	protein					1219:1225	protein	1219:1225	protein	1219:1225	Dehulling of wheat, barley and oat grains significantly contributed to the reduction of minerals, protein and total dietary fiber (TDF) contents, except for the amount of protein in dehulled wheat and oat grains.
33179481	8	35	theme	protein	1219:1225	arg1	amount					1209:1214	the amount	1205:1214	the amount of protein in dehulled wheat and oat grains	1205:1258	Dehulling of wheat, barley and oat grains significantly contributed to the reduction of minerals, protein and total dietary fiber (TDF) contents, except for the amount of protein in dehulled wheat and oat grains.
33179481	12	36	theme	tocochromanols	1725:1738	arg1	tocochromanols					1725:1738	tocochromanols	1725:1738	tocochromanols	1725:1738	Grain dehulling also significantly decreased the total amount of tocochromanols in relation to the original grains.
33179481	12	36	theme	tocochromanols	1725:1738	arg1	amount					1715:1720	the total amount	1705:1720	the total amount of tocochromanols in relation to the original grains	1705:1773	Grain dehulling also significantly decreased the total amount of tocochromanols in relation to the original grains.
33179481	1	37	theme	barley	62:67	arg1	Study					46:50	Study	46:50	Study of wheat, barley, oat and selected grain products.	46:101	Study of wheat, barley, oat and selected grain products.
33179481	14	38	theme	wheat	2009:2013	arg1	flour					2015:2019	white refined wheat flour	1995:2019	white refined wheat flour	1995:2019	They are characterized by high contents of tocochromanols, phenolic compounds and water-extractable arabinoxylans, with the exception of white refined wheat flour, which is mainly a source of saccharides.
33179481	3	39	theme	baking	330:335	arg1	industries					337:346	modern milling and baking industries	311:346	modern milling and baking industries	311:346	With this in mind, modern milling and baking industries keep introducing new types of products.
33179481	14	40	theme	arabinoxylans	1958:1970	arg1	contents					1889:1896	high contents	1884:1896	high contents of tocochromanols, phenolic compounds and water-extractable arabinoxylans	1884:1970	They are characterized by high contents of tocochromanols, phenolic compounds and water-extractable arabinoxylans, with the exception of white refined wheat flour, which is mainly a source of saccharides.
33179481	8	41	theme	dehulled	1230:1237	arg1	wheat					1239:1243	dehulled wheat and oat grains	1230:1258	wheat	1239:1243	Dehulling of wheat, barley and oat grains significantly contributed to the reduction of minerals, protein and total dietary fiber (TDF) contents, except for the amount of protein in dehulled wheat and oat grains.
33179481	5	42	theme	flour	713:717	arg1	bran					791:794	wheat bran	785:794	wheat bran	785:794	METHODS The aim of the study was to conduct a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour), as well as wheat bran.
33179481	5	42	theme	flour	713:717	arg1	evaluation					584:593	a qualitative and quantitative evaluation	553:593	a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour)	553:771	METHODS The aim of the study was to conduct a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour), as well as wheat bran.
33179481	9	43	theme	amounts	1392:1398	arg1	contents					1339:1346	the highest contents	1327:1346	the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch	1327:1430	Oat bran, in contrast to other oat products, was characterized by the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch.
33179481	6	44	theme	activity	945:952	arg1	contents					877:884	contents	877:884	contents	877:884	Additionally, analyses were performed to determine the chemical composition and contents of nutrients, selected bioactive compounds and antioxidant activity.
33179481	6	44	theme	activity	945:952	arg1	composition					861:871	chemical composition	852:871	chemical composition	852:871	Additionally, analyses were performed to determine the chemical composition and contents of nutrients, selected bioactive compounds and antioxidant activity.
33179481	3	45	theme	milling	318:324	arg1	industries					337:346	modern milling and baking industries	311:346	modern milling and baking industries	311:346	With this in mind, modern milling and baking industries keep introducing new types of products.
33179481	0	46	theme	bioactive	14:22	arg1	compounds					24:32	bioactive compounds	14:32	bioactive compounds	14:32	Evaluation of bioactive compounds in cereals.
33179481	1	47	theme	selected	78:85	arg1	products					93:100	selected grain products	78:100	selected grain products	78:100	Study of wheat, barley, oat and selected grain products.
33179481	3	48	theme	new	365:367	arg1	types					369:373	introducing new types	353:373	introducing new types of products	353:385	With this in mind, modern milling and baking industries keep introducing new types of products.
33179481	14	49	theme	phenolic	1917:1924	arg1	compounds					1926:1934	phenolic compounds	1917:1934	phenolic compounds	1917:1934	They are characterized by high contents of tocochromanols, phenolic compounds and water-extractable arabinoxylans, with the exception of white refined wheat flour, which is mainly a source of saccharides.
33179481	10	50	theme	flour	1486:1490	arg1	type					1492:1495	wheat flour type 550	1480:1499	wheat flour type 550	1480:1499	The lowest content of minerals was recorded in wheat flour type 550.
33179481	5	51	theme	ground	689:694	arg1	grain					696:700	ground grain	689:700	ground grain	689:700	METHODS The aim of the study was to conduct a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour), as well as wheat bran.
33179481	15	52	theme	oat	2089:2091	arg1	products					2093:2100	oat products	2089:2100	oat products	2089:2100	In the case of barley and oat products, the analysis showed no differences between these product groups.
33179481	9	53	theme	total	1419:1423	arg1	starch					1425:1430	total starch	1419:1430	total starch	1419:1430	Oat bran, in contrast to other oat products, was characterized by the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch.
33179481	6	54	theme	bioactive	909:917	arg1	compounds					919:927	selected bioactive compounds	900:927	selected bioactive compounds	900:927	Additionally, analyses were performed to determine the chemical composition and contents of nutrients, selected bioactive compounds and antioxidant activity.
33179481	14	55	theme	tocochromanols	1901:1914	arg1	contents					1889:1896	high contents	1884:1896	high contents of tocochromanols, phenolic compounds and water-extractable arabinoxylans	1884:1970	They are characterized by high contents of tocochromanols, phenolic compounds and water-extractable arabinoxylans, with the exception of white refined wheat flour, which is mainly a source of saccharides.
33179481	11	56	theme	Thermal	1502:1508	arg1	processes					1510:1518	Thermal processes	1502:1518	Thermal processes	1502:1518	Thermal processes affect the concentration of tocochromanols in the grain, with tocotrienols being more resistant to hydrothermal treatment than tocopherols.
33179481	16	57	theme	oat	2215:2217	arg1	dehulling					2191:2199	dehulling	2191:2199	dehulling of barley and oat grain	2191:2223	It was also shown that dehulling of barley and oat grain causes statistically significant differences in the contents of nutrients and natural antioxidants.
33179481	7	58	theme	chemical	1026:1033	arg1	composition					1035:1045	their chemical composition	1020:1045	their chemical composition	1020:1045	RESULTS The studied raw materials in commercial cereal differ in their chemical composition.
33179481	15	59	theme	barley	2078:2083	arg1	case					2070:2073	the case	2066:2073	the case of barley and oat products	2066:2100	In the case of barley and oat products, the analysis showed no differences between these product groups.
33179481	12	60	theme	Grain	1660:1664	arg1	dehulling					1666:1674	Grain dehulling	1660:1674	Grain dehulling	1660:1674	Grain dehulling also significantly decreased the total amount of tocochromanols in relation to the original grains.
33179481	2	61	theme	good	178:181	arg1	health					183:188	good health	178:188	good health	178:188	BACKGROUND One of the requirements for proper nutrition and maintenance of good health is to supply the body, through diet, with an appropriately increased quantity of bioactive compounds.
33179481	9	62	dep	products	1296:1303	arg1	contrast					1274:1281	contrast	1274:1281	contrast	1274:1281	Oat bran, in contrast to other oat products, was characterized by the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch.
33179481	13	63	theme	individual	1835:1844	arg1	components					1846:1855	their individual components	1829:1855	their individual components	1829:1855	CONCLUSIONS Wheat products differ in the contents of their individual components.
33179481	15	64	theme	product	2152:2158	arg1	groups					2160:2165	these product groups	2146:2165	these product groups	2146:2165	In the case of barley and oat products, the analysis showed no differences between these product groups.
33179481	16	65	theme	antioxidants	2311:2322	arg1	contents					2277:2284	the contents	2273:2284	the contents of nutrients and natural antioxidants	2273:2322	It was also shown that dehulling of barley and oat grain causes statistically significant differences in the contents of nutrients and natural antioxidants.
33179481	9	66	theme	oat	1292:1294	arg1	products					1296:1303	other oat products	1286:1303	other oat products	1286:1303	Oat bran, in contrast to other oat products, was characterized by the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch.
33179481	8	67	theme	wheat	1061:1065	arg1	grains					1083:1088	wheat, barley and oat grains	1061:1088	wheat, barley and oat grains	1061:1088	Dehulling of wheat, barley and oat grains significantly contributed to the reduction of minerals, protein and total dietary fiber (TDF) contents, except for the amount of protein in dehulled wheat and oat grains.
33179481	7	68	theme	studied	967:973	arg1	materials					979:987	The studied raw materials	963:987	The studied raw materials	963:987	RESULTS The studied raw materials in commercial cereal differ in their chemical composition.
33179481	13	69	theme	Wheat	1788:1792	arg1	products					1794:1801	CONCLUSIONS Wheat products	1776:1801	CONCLUSIONS Wheat products	1776:1801	CONCLUSIONS Wheat products differ in the contents of their individual components.
33179481	2	70	theme	bioactive	271:279	arg1	compounds					281:289	bioactive compounds	271:289	bioactive compounds	271:289	BACKGROUND One of the requirements for proper nutrition and maintenance of good health is to supply the body, through diet, with an appropriately increased quantity of bioactive compounds.
33179481	5	71	theme	plain	760:764	arg1	flour					766:770	plain flour	760:770	plain flour	760:770	METHODS The aim of the study was to conduct a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour), as well as wheat bran.
33179481	8	72	theme	barley	1068:1073	arg1	grains					1083:1088	wheat, barley and oat grains	1061:1088	wheat, barley and oat grains	1061:1088	Dehulling of wheat, barley and oat grains significantly contributed to the reduction of minerals, protein and total dietary fiber (TDF) contents, except for the amount of protein in dehulled wheat and oat grains.
33179481	7	73	theme	raw	975:977	arg1	materials					979:987	The studied raw materials	963:987	The studied raw materials	963:987	RESULTS The studied raw materials in commercial cereal differ in their chemical composition.
33179481	8	74	theme	minerals	1136:1143	arg1	reduction					1123:1131	the reduction	1119:1131	the reduction of minerals, protein and total dietary fiber (TDF) contents	1119:1191	Dehulling of wheat, barley and oat grains significantly contributed to the reduction of minerals, protein and total dietary fiber (TDF) contents, except for the amount of protein in dehulled wheat and oat grains.
33179481	16	75	theme	barley	2204:2209	arg1	dehulling					2191:2199	dehulling	2191:2199	dehulling of barley and oat grain	2191:2223	It was also shown that dehulling of barley and oat grain causes statistically significant differences in the contents of nutrients and natural antioxidants.
33179481	5	76	theme	flour	729:733	arg1	type					735:738	wheat flour type 550	723:742	wheat flour type 550 (all-purpose or plain flour)	723:771	METHODS The aim of the study was to conduct a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour), as well as wheat bran.
33179481	8	77	theme	oat	1079:1081	arg1	grains					1083:1088	wheat, barley and oat grains	1061:1088	wheat, barley and oat grains	1061:1088	Dehulling of wheat, barley and oat grains significantly contributed to the reduction of minerals, protein and total dietary fiber (TDF) contents, except for the amount of protein in dehulled wheat and oat grains.
33179481	8	78	theme	protein	1146:1152	arg1	reduction					1123:1131	the reduction	1119:1131	the reduction of minerals, protein and total dietary fiber (TDF) contents	1119:1191	Dehulling of wheat, barley and oat grains significantly contributed to the reduction of minerals, protein and total dietary fiber (TDF) contents, except for the amount of protein in dehulled wheat and oat grains.
33179481	4	79	theme	additives	404:412	arg1	use					392:394	The use	388:394	The use of such additives as wholegrain flours and bran in baked products	388:460	The use of such additives as wholegrain flours and bran in baked products provided the basis for research in this paper.
33179481	8	80	theme	total	1158:1162	arg1	contents					1184:1191	total dietary fiber (TDF) contents	1158:1191	total dietary fiber (TDF) contents	1158:1191	Dehulling of wheat, barley and oat grains significantly contributed to the reduction of minerals, protein and total dietary fiber (TDF) contents, except for the amount of protein in dehulled wheat and oat grains.
33179481	7	81	dep	materials	979:987	arg1	cereal					1003:1008	cereal	1003:1008	cereal	1003:1008	RESULTS The studied raw materials in commercial cereal differ in their chemical composition.
33179481	5	82	theme	marketable	598:607	arg1	wheat					609:613	marketable wheat	598:613	marketable wheat	598:613	METHODS The aim of the study was to conduct a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour), as well as wheat bran.
33179481	1	83	theme	grain	87:91	arg1	products					93:100	selected grain products	78:100	selected grain products	78:100	Study of wheat, barley, oat and selected grain products.
33179481	4	84	theme	baked	447:451	arg1	products					453:460	baked products	447:460	baked products	447:460	The use of such additives as wholegrain flours and bran in baked products provided the basis for research in this paper.
33179481	2	85	with	diet	221:224	arg1	quantity					259:266	an appropriately increased quantity	232:266	an appropriately increased quantity of bioactive compounds	232:289	BACKGROUND One of the requirements for proper nutrition and maintenance of good health is to supply the body, through diet, with an appropriately increased quantity of bioactive compounds.
33179481	11	86	theme	tocochromanols	1548:1561	arg1	concentration					1531:1543	the concentration	1527:1543	the concentration of tocochromanols in the grain	1527:1574	Thermal processes affect the concentration of tocochromanols in the grain, with tocotrienols being more resistant to hydrothermal treatment than tocopherols.
33179481	8	87	theme	fiber	1172:1176	arg1	contents					1184:1191	total dietary fiber (TDF) contents	1158:1191	total dietary fiber (TDF) contents	1158:1191	Dehulling of wheat, barley and oat grains significantly contributed to the reduction of minerals, protein and total dietary fiber (TDF) contents, except for the amount of protein in dehulled wheat and oat grains.
33179481	6	88	theme	selected	900:907	arg1	compounds					919:927	selected bioactive compounds	900:927	selected bioactive compounds	900:927	Additionally, analyses were performed to determine the chemical composition and contents of nutrients, selected bioactive compounds and antioxidant activity.
33179481	8	89	theme	TDF	1179:1181	arg1	contents					1184:1191	total dietary fiber (TDF) contents	1158:1191	total dietary fiber (TDF) contents	1158:1191	Dehulling of wheat, barley and oat grains significantly contributed to the reduction of minerals, protein and total dietary fiber (TDF) contents, except for the amount of protein in dehulled wheat and oat grains.
33179481	9	90	theme	minerals	1351:1358	arg1	contents					1339:1346	the highest contents	1327:1346	the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch	1327:1430	Oat bran, in contrast to other oat products, was characterized by the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch.
33179481	6	91	theme	nutrients	889:897	arg1	contents					877:884	contents	877:884	contents	877:884	Additionally, analyses were performed to determine the chemical composition and contents of nutrients, selected bioactive compounds and antioxidant activity.
33179481	6	91	theme	nutrients	889:897	arg1	composition					861:871	chemical composition	852:871	chemical composition	852:871	Additionally, analyses were performed to determine the chemical composition and contents of nutrients, selected bioactive compounds and antioxidant activity.
33179481	11	92	from	concentration	1531:1543	arg1	grain					1570:1574	the grain	1566:1574	the grain	1566:1574	Thermal processes affect the concentration of tocochromanols in the grain, with tocotrienols being more resistant to hydrothermal treatment than tocopherols.
33179481	6	93	dep	composition	861:871	arg1	the					848:850	the	848:850	the	848:850	Additionally, analyses were performed to determine the chemical composition and contents of nutrients, selected bioactive compounds and antioxidant activity.
33179481	8	94	theme	contents	1184:1191	arg1	reduction					1123:1131	the reduction	1119:1131	the reduction of minerals, protein and total dietary fiber (TDF) contents	1119:1191	Dehulling of wheat, barley and oat grains significantly contributed to the reduction of minerals, protein and total dietary fiber (TDF) contents, except for the amount of protein in dehulled wheat and oat grains.
33179481	5	95	theme	dehulled	671:678	arg1	kernels					680:686	dehulled kernels	671:686	dehulled kernels	671:686	METHODS The aim of the study was to conduct a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour), as well as wheat bran.
33179481	12	96	theme	original	1759:1766	arg1	grains					1768:1773	the original grains	1755:1773	the original grains	1755:1773	Grain dehulling also significantly decreased the total amount of tocochromanols in relation to the original grains.
33179481	9	97	theme	protein	1361:1367	arg1	contents					1339:1346	the highest contents	1327:1346	the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch	1327:1430	Oat bran, in contrast to other oat products, was characterized by the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch.
33179481	16	98	dep	barley	2204:2209	arg1	grain					2219:2223	grain	2219:2223	grain	2219:2223	It was also shown that dehulling of barley and oat grain causes statistically significant differences in the contents of nutrients and natural antioxidants.
33179481	9	99	theme	TDF	1370:1372	arg1	contents					1339:1346	the highest contents	1327:1346	the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch	1327:1430	Oat bran, in contrast to other oat products, was characterized by the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch.
33179481	3	100	theme	modern	311:316	arg1	industries					337:346	modern milling and baking industries	311:346	modern milling and baking industries	311:346	With this in mind, modern milling and baking industries keep introducing new types of products.
33179481	4	101	theme	wholegrain	417:426	arg1	flours					428:433	wholegrain flours	417:433	wholegrain flours	417:433	The use of such additives as wholegrain flours and bran in baked products provided the basis for research in this paper.
33179481	5	102	theme	raw	646:648	arg1	materials					650:658	raw materials	646:658	raw materials	646:658	METHODS The aim of the study was to conduct a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour), as well as wheat bran.
33179481	9	103	theme	highest	1331:1337	arg1	contents					1339:1346	the highest contents	1327:1346	the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch	1327:1430	Oat bran, in contrast to other oat products, was characterized by the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch.
33179481	5	104	theme	wholemeal	703:711	arg1	flour					713:717	wholemeal flour	703:717	wholemeal flour	703:717	METHODS The aim of the study was to conduct a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour), as well as wheat bran.
33179481	9	105	theme	smallest	1383:1390	arg1	starch					1425:1430	total starch	1419:1430	total starch	1419:1430	Oat bran, in contrast to other oat products, was characterized by the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch.
33179481	9	105	theme	smallest	1383:1390	arg1	amounts					1392:1398	the smallest amounts	1379:1398	the smallest amounts of saccharides and total starch	1379:1430	Oat bran, in contrast to other oat products, was characterized by the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch.
33179481	9	105	theme	smallest	1383:1390	arg1	saccharides					1403:1413	saccharides	1403:1413	saccharides	1403:1413	Oat bran, in contrast to other oat products, was characterized by the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch.
33179481	14	106	theme	water-extractable	1940:1956	arg1	arabinoxylans					1958:1970	water-extractable arabinoxylans	1940:1970	water-extractable arabinoxylans	1940:1970	They are characterized by high contents of tocochromanols, phenolic compounds and water-extractable arabinoxylans, with the exception of white refined wheat flour, which is mainly a source of saccharides.
33179481	3	107	theme	products	378:385	arg1	types					369:373	introducing new types	353:373	introducing new types of products	353:385	With this in mind, modern milling and baking industries keep introducing new types of products.
33179481	0	108	theme	compounds	24:32	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of bioactive compounds in cereals.	0:44	Evaluation of bioactive compounds in cereals.
33179481	12	109	theme	total	1709:1713	arg1	tocochromanols					1725:1738	tocochromanols	1725:1738	tocochromanols	1725:1738	Grain dehulling also significantly decreased the total amount of tocochromanols in relation to the original grains.
33179481	12	109	theme	total	1709:1713	arg1	amount					1715:1720	the total amount	1705:1720	the total amount of tocochromanols in relation to the original grains	1705:1773	Grain dehulling also significantly decreased the total amount of tocochromanols in relation to the original grains.
33179481	6	110	theme	antioxidant	933:943	arg1	activity					945:952	antioxidant activity	933:952	antioxidant activity	933:952	Additionally, analyses were performed to determine the chemical composition and contents of nutrients, selected bioactive compounds and antioxidant activity.
33179481	16	111	theme	significant	2246:2256	arg1	differences					2258:2268	statistically significant differences	2232:2268	statistically significant differences	2232:2268	It was also shown that dehulling of barley and oat grain causes statistically significant differences in the contents of nutrients and natural antioxidants.
33179481	14	112	theme	compounds	1926:1934	arg1	contents					1889:1896	high contents	1884:1896	high contents of tocochromanols, phenolic compounds and water-extractable arabinoxylans	1884:1970	They are characterized by high contents of tocochromanols, phenolic compounds and water-extractable arabinoxylans, with the exception of white refined wheat flour, which is mainly a source of saccharides.
33179481	6	113	theme	compounds	919:927	arg1	contents					877:884	contents	877:884	contents	877:884	Additionally, analyses were performed to determine the chemical composition and contents of nutrients, selected bioactive compounds and antioxidant activity.
33179481	6	113	theme	compounds	919:927	arg1	composition					861:871	chemical composition	852:871	chemical composition	852:871	Additionally, analyses were performed to determine the chemical composition and contents of nutrients, selected bioactive compounds and antioxidant activity.
33179481	2	114	theme	proper	142:147	arg1	nutrition					149:157	proper nutrition and maintenance	142:173	nutrition	149:157	BACKGROUND One of the requirements for proper nutrition and maintenance of good health is to supply the body, through diet, with an appropriately increased quantity of bioactive compounds.
33179481	8	115	theme	oat	1249:1251	arg1	grains					1253:1258	dehulled wheat and oat grains	1230:1258	grains	1253:1258	Dehulling of wheat, barley and oat grains significantly contributed to the reduction of minerals, protein and total dietary fiber (TDF) contents, except for the amount of protein in dehulled wheat and oat grains.
33179481	10	116	theme	wheat	1480:1484	arg1	type					1492:1495	wheat flour type 550	1480:1499	wheat flour type 550	1480:1499	The lowest content of minerals was recorded in wheat flour type 550.
33179481	5	117	theme	grain	696:700	arg1	bran					791:794	wheat bran	785:794	wheat bran	785:794	METHODS The aim of the study was to conduct a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour), as well as wheat bran.
33179481	5	117	theme	grain	696:700	arg1	evaluation					584:593	a qualitative and quantitative evaluation	553:593	a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour)	553:771	METHODS The aim of the study was to conduct a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour), as well as wheat bran.
33179481	15	118	theme	products	2093:2100	arg1	case					2070:2073	the case	2066:2073	the case of barley and oat products	2066:2100	In the case of barley and oat products, the analysis showed no differences between these product groups.
33179481	9	119	theme	starch	1425:1430	arg1	starch					1425:1430	total starch	1419:1430	total starch	1419:1430	Oat bran, in contrast to other oat products, was characterized by the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch.
33179481	9	119	theme	starch	1425:1430	arg1	protein					1361:1367	protein	1361:1367	protein	1361:1367	Oat bran, in contrast to other oat products, was characterized by the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch.
33179481	9	119	theme	starch	1425:1430	arg1	amounts					1392:1398	the smallest amounts	1379:1398	the smallest amounts of saccharides and total starch	1379:1430	Oat bran, in contrast to other oat products, was characterized by the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch.
33179481	9	119	theme	starch	1425:1430	arg1	saccharides					1403:1413	saccharides	1403:1413	saccharides	1403:1413	Oat bran, in contrast to other oat products, was characterized by the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch.
33179481	9	119	theme	starch	1425:1430	arg1	TDF					1370:1372	TDF	1370:1372	TDF	1370:1372	Oat bran, in contrast to other oat products, was characterized by the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch.
33179481	9	119	theme	starch	1425:1430	arg1	minerals					1351:1358	minerals	1351:1358	minerals	1351:1358	Oat bran, in contrast to other oat products, was characterized by the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch.
33179481	3	120	theme	introducing	353:363	arg1	types					369:373	introducing new types	353:373	introducing new types of products	353:385	With this in mind, modern milling and baking industries keep introducing new types of products.
33179481	2	121	theme	health	183:188	arg1	nutrition					149:157	proper nutrition and maintenance	142:173	nutrition	149:157	BACKGROUND One of the requirements for proper nutrition and maintenance of good health is to supply the body, through diet, with an appropriately increased quantity of bioactive compounds.
33179481	2	121	theme	health	183:188	arg1	maintenance					163:173	proper nutrition and maintenance	142:173	maintenance	163:173	BACKGROUND One of the requirements for proper nutrition and maintenance of good health is to supply the body, through diet, with an appropriately increased quantity of bioactive compounds.
33179481	14	122	theme	high	1884:1887	arg1	contents					1889:1896	high contents	1884:1896	high contents of tocochromanols, phenolic compounds and water-extractable arabinoxylans	1884:1970	They are characterized by high contents of tocochromanols, phenolic compounds and water-extractable arabinoxylans, with the exception of white refined wheat flour, which is mainly a source of saccharides.
33179481	4	123	from	research	485:492	arg1	paper					502:506	this paper	497:506	this paper	497:506	The use of such additives as wholegrain flours and bran in baked products provided the basis for research in this paper.
33179481	2	124	theme	compounds	281:289	arg1	quantity					259:266	an appropriately increased quantity	232:266	an appropriately increased quantity of bioactive compounds	232:289	BACKGROUND One of the requirements for proper nutrition and maintenance of good health is to supply the body, through diet, with an appropriately increased quantity of bioactive compounds.
33179481	8	125	from	amount	1209:1214	arg1	wheat					1239:1243	dehulled wheat and oat grains	1230:1258	wheat	1239:1243	Dehulling of wheat, barley and oat grains significantly contributed to the reduction of minerals, protein and total dietary fiber (TDF) contents, except for the amount of protein in dehulled wheat and oat grains.
33179481	8	125	from	amount	1209:1214	arg1	grains					1253:1258	dehulled wheat and oat grains	1230:1258	grains	1253:1258	Dehulling of wheat, barley and oat grains significantly contributed to the reduction of minerals, protein and total dietary fiber (TDF) contents, except for the amount of protein in dehulled wheat and oat grains.
33179481	10	126	theme	lowest	1437:1442	arg1	content					1444:1450	The lowest content	1433:1450	The lowest content of minerals	1433:1462	The lowest content of minerals was recorded in wheat flour type 550.
33179481	5	127	dep	type	735:738	arg1	all-purpose					745:755	all-purpose	745:755	all-purpose	745:755	METHODS The aim of the study was to conduct a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour), as well as wheat bran.
33179481	5	127	dep	type	735:738	arg1	flour					766:770	plain flour	760:770	plain flour	760:770	METHODS The aim of the study was to conduct a qualitative and quantitative evaluation of marketable wheat, barley and oat grain, used as raw materials to produce dehulled kernels, ground grain, wholemeal flour and wheat flour type 550 (all-purpose or plain flour), as well as wheat bran.
33179481	13	128	theme	components	1846:1855	arg1	contents					1817:1824	the contents	1813:1824	the contents of their individual components	1813:1855	CONCLUSIONS Wheat products differ in the contents of their individual components.
33179481	9	129	theme	Oat	1261:1263	arg1	bran					1265:1268	Oat bran	1261:1268	Oat bran	1261:1268	Oat bran, in contrast to other oat products, was characterized by the highest contents of minerals, protein, TDF, and the smallest amounts of saccharides and total starch.
33325943	8	0	theme	UPLC	1094:1097	arg1	results					1099:1105	the UPLC results	1090:1105	the UPLC results	1090:1105	LC-Mass spectrometry (MS) analysis of the N-linked glycans and lectin blotting of samples was carried out to confirm the UPLC results.
33325943	7	1	theme	liquid	829:834	arg1	UPLC					852:855	UPLC	852:855	UPLC	852:855	Ultra-performance liquid chromatography (UPLC) analysis of exoglycosidase digested N-linked glycans was carried out on TIS compared to non-treated control cells.
33325943	7	1	theme	liquid	829:834	arg1	chromatography					836:849	Ultra-performance liquid chromatography	811:849	Ultra-performance liquid chromatography (UPLC) analysis of exoglycosidase digested N-linked glycans	811:909	Ultra-performance liquid chromatography (UPLC) analysis of exoglycosidase digested N-linked glycans was carried out on TIS compared to non-treated control cells.
33325943	13	2	theme	EV	1926:1927	arg1	progeny					1929:1935	their resultant EV progeny	1910:1935	their resultant EV progeny	1910:1935	This study has shown that therapeutic-induced-senescent TNBC cells and their EV progeny, display differential N-glycan moieties compared to non-senescent Cal51 cells and their resultant EV progeny.
33325943	13	3	theme	N-glycan	1850:1857	arg1	moieties					1859:1866	differential N-glycan moieties	1837:1866	differential N-glycan moieties	1837:1866	This study has shown that therapeutic-induced-senescent TNBC cells and their EV progeny, display differential N-glycan moieties compared to non-senescent Cal51 cells and their resultant EV progeny.
33325943	11	4	theme	non-senescent	1573:1585	arg1	cells					1595:1599	non-senescent control cells	1573:1599	non-senescent control cells with a major 32 kDa band only	1573:1629	The lectin, calnexin demonstrated a lower kDa size (∼58 kDa) in TIS compared to control cells (∼90 kDa) while Galectin 3 demonstrated potential proteolytic cleavage with 32 kDa and ∼22 kDa bands evident in TIS compared to non-senescent control cells with a major 32 kDa band only.
33325943	10	5	theme	modifying	1332:1340	arg1	enzymes					1342:1348	different glycan modifying enzymes	1315:1348	different glycan modifying enzymes	1315:1348	Protein mass spectrometry showed that the TIS cells contain different glycan modifying enzymes.
33325943	1	6	theme	limited	228:234	arg1	options					246:252	limited treatment options	228:252	limited treatment options	228:252	Triple negative breast cancer (TNBC) has poor clinical outcomes and limited treatment options.
33325943	9	7	theme	N-glycan	1150:1157	arg1	profile					1159:1165	the N-glycan profile	1146:1165	the N-glycan profile of the Cal51 membrane, cytoplasm and EV progeny of TIS compared to non-senescent cells	1146:1252	Significant differences were found in the N-glycan profile of the Cal51 membrane, cytoplasm and EV progeny of TIS compared to non-senescent cells.
33325943	0	8	theme	extracellular	124:136	arg1	vesicle					138:144	their extracellular vesicle	118:144	their extracellular vesicle (EV) progeny	118:157	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.
33325943	0	8	theme	extracellular	124:136	arg1	EV					147:148	EV	147:148	EV	147:148	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.
33325943	14	9	theme	response	2070:2077	arg1	monitoring					2046:2055	the monitoring	2042:2055	(i) the monitoring of treatment response as a liquid biopsy	2038:2096	For the future, N-glycan moieties on cancer senescent cells and their EV progeny hold potential for (i) the monitoring of treatment response as a liquid biopsy, and (ii) cancer senescent cell targeting with lectin therapies.
33325943	14	9	theme	response	2070:2077	arg1	targeting					2130:2138	(ii) cancer senescent cell targeting	2103:2138	(ii) cancer senescent cell targeting	2103:2138	For the future, N-glycan moieties on cancer senescent cells and their EV progeny hold potential for (i) the monitoring of treatment response as a liquid biopsy, and (ii) cancer senescent cell targeting with lectin therapies.
33325943	11	10	with	cells	1595:1599	arg1	band					1621:1624	a major 32 kDa band	1606:1624	a major 32 kDa band only	1606:1629	The lectin, calnexin demonstrated a lower kDa size (∼58 kDa) in TIS compared to control cells (∼90 kDa) while Galectin 3 demonstrated potential proteolytic cleavage with 32 kDa and ∼22 kDa bands evident in TIS compared to non-senescent control cells with a major 32 kDa band only.
33325943	1	11	theme	Triple	160:165	arg1	TNBC					191:194	TNBC	191:194	TNBC	191:194	Triple negative breast cancer (TNBC) has poor clinical outcomes and limited treatment options.
33325943	1	11	theme	Triple	160:165	arg1	cancer					183:188	Triple negative breast cancer	160:188	Triple negative breast cancer (TNBC)	160:195	Triple negative breast cancer (TNBC) has poor clinical outcomes and limited treatment options.
33325943	4	12	link	O-linked	474:481	arg1	glycosylation					483:495	O-linked glycosylation	474:495	O-linked glycosylation	474:495	Recently, N- and O-linked glycosylation alterations have been associated with senescence.
33325943	8	13	theme	LC-Mass	973:979	arg1	MS					995:996	MS	995:996	MS	995:996	LC-Mass spectrometry (MS) analysis of the N-linked glycans and lectin blotting of samples was carried out to confirm the UPLC results.
33325943	8	13	theme	LC-Mass	973:979	arg1	spectrometry					981:992	LC-Mass spectrometry	973:992	LC-Mass spectrometry (MS) analysis of the N-linked glycans	973:1030	LC-Mass spectrometry (MS) analysis of the N-linked glycans and lectin blotting of samples was carried out to confirm the UPLC results.
33325943	9	14	theme	Cal51	1174:1178	arg1	membrane					1180:1187	the Cal51 membrane	1170:1187	the Cal51 membrane	1170:1187	Significant differences were found in the N-glycan profile of the Cal51 membrane, cytoplasm and EV progeny of TIS compared to non-senescent cells.
33325943	1	15	theme	breast	176:181	arg1	TNBC					191:194	TNBC	191:194	TNBC	191:194	Triple negative breast cancer (TNBC) has poor clinical outcomes and limited treatment options.
33325943	1	15	theme	breast	176:181	arg1	cancer					183:188	Triple negative breast cancer	160:188	Triple negative breast cancer (TNBC)	160:195	Triple negative breast cancer (TNBC) has poor clinical outcomes and limited treatment options.
33325943	1	16	contain	has	197:199	arg2	outcomes					215:222	poor clinical outcomes	201:222	poor clinical outcomes	201:222	Triple negative breast cancer (TNBC) has poor clinical outcomes and limited treatment options.
33325943	1	16	contain	has	197:199	arg1	cancer					183:188	Triple negative breast cancer	160:188	Triple negative breast cancer (TNBC)	160:195	Triple negative breast cancer (TNBC) has poor clinical outcomes and limited treatment options.
33325943	1	16	contain	has	197:199	arg1	TNBC					191:194	TNBC	191:194	TNBC	191:194	Triple negative breast cancer (TNBC) has poor clinical outcomes and limited treatment options.
33325943	1	16	contain	has	197:199	arg2	options					246:252	limited treatment options	228:252	limited treatment options	228:252	Triple negative breast cancer (TNBC) has poor clinical outcomes and limited treatment options.
33325943	5	17	theme	TIS	647:649	arg1	cells					656:660	TIS TNBC cells	647:660	TIS TNBC cells	647:660	We aimed to profile the N-linked glycans of whole cells, membrane, cytoplasm and EVs harvested from TIS TNBC cells and to compare these to results from non-senescent cells.
33325943	9	18	located	found	1137:1141	arg2	differences					1120:1130	Significant differences	1108:1130	Significant differences	1108:1130	Significant differences were found in the N-glycan profile of the Cal51 membrane, cytoplasm and EV progeny of TIS compared to non-senescent cells.
33325943	9	18	located	found	1137:1141	arg1	profile					1159:1165	the N-glycan profile	1146:1165	the N-glycan profile of the Cal51 membrane, cytoplasm and EV progeny of TIS compared to non-senescent cells	1146:1252	Significant differences were found in the N-glycan profile of the Cal51 membrane, cytoplasm and EV progeny of TIS compared to non-senescent cells.
33325943	9	19	theme	progeny	1207:1213	arg1	profile					1159:1165	the N-glycan profile	1146:1165	the N-glycan profile of the Cal51 membrane, cytoplasm and EV progeny of TIS compared to non-senescent cells	1146:1252	Significant differences were found in the N-glycan profile of the Cal51 membrane, cytoplasm and EV progeny of TIS compared to non-senescent cells.
33325943	7	20	theme	non-treated	946:956	arg1	cells					966:970	non-treated control cells	946:970	non-treated control cells	946:970	Ultra-performance liquid chromatography (UPLC) analysis of exoglycosidase digested N-linked glycans was carried out on TIS compared to non-treated control cells.
33325943	14	21	theme	cell	2125:2128	arg1	targeting					2130:2138	(ii) cancer senescent cell targeting	2103:2138	(ii) cancer senescent cell targeting	2103:2138	For the future, N-glycan moieties on cancer senescent cells and their EV progeny hold potential for (i) the monitoring of treatment response as a liquid biopsy, and (ii) cancer senescent cell targeting with lectin therapies.
33325943	0	22	theme	TIS	66:68	arg1	TNBC					108:111	TNBC	108:111	TNBC	108:111	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.
33325943	0	22	theme	TIS	66:68	arg1	cells					101:105	therapeutic induced senescent (TIS) triple negative breast cancer cells	35:105	therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC)	35:112	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.
33325943	9	23	theme	TIS	1218:1220	arg1	cytoplasm					1190:1198	cytoplasm	1190:1198	cytoplasm	1190:1198	Significant differences were found in the N-glycan profile of the Cal51 membrane, cytoplasm and EV progeny of TIS compared to non-senescent cells.
33325943	9	23	theme	TIS	1218:1220	arg1	progeny					1207:1213	EV progeny	1204:1213	EV progeny	1204:1213	Significant differences were found in the N-glycan profile of the Cal51 membrane, cytoplasm and EV progeny of TIS compared to non-senescent cells.
33325943	9	23	theme	TIS	1218:1220	arg1	membrane					1180:1187	the Cal51 membrane	1170:1187	the Cal51 membrane	1170:1187	Significant differences were found in the N-glycan profile of the Cal51 membrane, cytoplasm and EV progeny of TIS compared to non-senescent cells.
33325943	4	24	theme	N-	467:468	arg1	alterations					497:507	N- and O-linked glycosylation alterations	467:507	N- and O-linked glycosylation alterations	467:507	Recently, N- and O-linked glycosylation alterations have been associated with senescence.
33325943	0	25	theme	triple	71:76	arg1	TNBC					108:111	TNBC	108:111	TNBC	108:111	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.
33325943	0	25	theme	triple	71:76	arg1	cells					101:105	therapeutic induced senescent (TIS) triple negative breast cancer cells	35:105	therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC)	35:112	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.
33325943	5	26	from	cells	713:717	arg1	results					686:692	results	686:692	results from non-senescent cells	686:717	We aimed to profile the N-linked glycans of whole cells, membrane, cytoplasm and EVs harvested from TIS TNBC cells and to compare these to results from non-senescent cells.
33325943	5	27	theme	N-linked	571:578	arg1	glycans					580:586	the N-linked glycans	567:586	the N-linked glycans of whole cells, membrane, cytoplasm and EVs harvested from TIS TNBC cells	567:660	We aimed to profile the N-linked glycans of whole cells, membrane, cytoplasm and EVs harvested from TIS TNBC cells and to compare these to results from non-senescent cells.
33325943	4	28	theme	O-linked	474:481	arg1	glycosylation					483:495	O-linked glycosylation	474:495	O-linked glycosylation	474:495	Recently, N- and O-linked glycosylation alterations have been associated with senescence.
33325943	13	29	theme	Cal51	1894:1898	arg1	cells					1900:1904	non-senescent Cal51 cells	1880:1904	non-senescent Cal51 cells	1880:1904	This study has shown that therapeutic-induced-senescent TNBC cells and their EV progeny, display differential N-glycan moieties compared to non-senescent Cal51 cells and their resultant EV progeny.
33325943	11	30	theme	control	1431:1437	arg1	cells					1439:1443	control cells	1431:1443	control cells (∼90 kDa)	1431:1453	The lectin, calnexin demonstrated a lower kDa size (∼58 kDa) in TIS compared to control cells (∼90 kDa) while Galectin 3 demonstrated potential proteolytic cleavage with 32 kDa and ∼22 kDa bands evident in TIS compared to non-senescent control cells with a major 32 kDa band only.
33325943	11	30	theme	control	1431:1437	arg1	kDa					1450:1452	∼90 kDa	1446:1452	∼90 kDa	1446:1452	The lectin, calnexin demonstrated a lower kDa size (∼58 kDa) in TIS compared to control cells (∼90 kDa) while Galectin 3 demonstrated potential proteolytic cleavage with 32 kDa and ∼22 kDa bands evident in TIS compared to non-senescent control cells with a major 32 kDa band only.
33325943	5	31	link	N-linked	571:578	arg1	glycans					580:586	the N-linked glycans	567:586	the N-linked glycans of whole cells, membrane, cytoplasm and EVs harvested from TIS TNBC cells	567:660	We aimed to profile the N-linked glycans of whole cells, membrane, cytoplasm and EVs harvested from TIS TNBC cells and to compare these to results from non-senescent cells.
33325943	0	32	theme	breast	87:92	arg1	TNBC					108:111	TNBC	108:111	TNBC	108:111	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.
33325943	0	32	theme	breast	87:92	arg1	cells					101:105	therapeutic induced senescent (TIS) triple negative breast cancer cells	35:105	therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC)	35:112	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.
33325943	11	33	theme	kDa	1393:1395	arg1	size					1397:1400	a lower kDa size	1385:1400	a lower kDa size (∼58 kDa) in TIS compared to control cells (∼90 kDa) while Galectin 3	1385:1470	The lectin, calnexin demonstrated a lower kDa size (∼58 kDa) in TIS compared to control cells (∼90 kDa) while Galectin 3 demonstrated potential proteolytic cleavage with 32 kDa and ∼22 kDa bands evident in TIS compared to non-senescent control cells with a major 32 kDa band only.
33325943	11	33	theme	kDa	1393:1395	arg1	kDa					1407:1409	∼58 kDa	1403:1409	∼58 kDa	1403:1409	The lectin, calnexin demonstrated a lower kDa size (∼58 kDa) in TIS compared to control cells (∼90 kDa) while Galectin 3 demonstrated potential proteolytic cleavage with 32 kDa and ∼22 kDa bands evident in TIS compared to non-senescent control cells with a major 32 kDa band only.
33325943	0	34	theme	cells	101:105	arg1	profiles					23:30	N-Linked glycosylation profiles	0:30	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.	0:158	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.
33325943	7	35	theme	glycans	903:909	arg1	analysis					858:865	Ultra-performance liquid chromatography (UPLC) analysis	811:865	Ultra-performance liquid chromatography (UPLC) analysis of exoglycosidase digested N-linked glycans	811:909	Ultra-performance liquid chromatography (UPLC) analysis of exoglycosidase digested N-linked glycans was carried out on TIS compared to non-treated control cells.
33325943	13	36	theme	therapeutic-induced-senescent	1766:1794	arg1	cells					1801:1805	therapeutic-induced-senescent TNBC cells	1766:1805	therapeutic-induced-senescent TNBC cells	1766:1805	This study has shown that therapeutic-induced-senescent TNBC cells and their EV progeny, display differential N-glycan moieties compared to non-senescent Cal51 cells and their resultant EV progeny.
33325943	3	37	theme	more	397:400	arg1	vesicles					416:423	significantly more extracellular vesicles	383:423	significantly more extracellular vesicles (EVs)	383:429	Senescent cells release significantly more extracellular vesicles (EVs) than non-senescent cells.
33325943	3	37	theme	more	397:400	arg1	EVs					426:428	EVs	426:428	EVs	426:428	Senescent cells release significantly more extracellular vesicles (EVs) than non-senescent cells.
33325943	0	38	theme	N-Linked	0:7	arg1	profiles					23:30	N-Linked glycosylation profiles	0:30	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.	0:158	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.
33325943	1	39	theme	treatment	236:244	arg1	options					246:252	limited treatment options	228:252	limited treatment options	228:252	Triple negative breast cancer (TNBC) has poor clinical outcomes and limited treatment options.
33325943	8	40	theme	N-linked	1015:1022	arg1	glycans					1024:1030	the N-linked glycans	1011:1030	the N-linked glycans	1011:1030	LC-Mass spectrometry (MS) analysis of the N-linked glycans and lectin blotting of samples was carried out to confirm the UPLC results.
33325943	7	41	theme	digested	885:892	arg1	glycans					903:909	exoglycosidase digested N-linked glycans	870:909	exoglycosidase digested N-linked glycans	870:909	Ultra-performance liquid chromatography (UPLC) analysis of exoglycosidase digested N-linked glycans was carried out on TIS compared to non-treated control cells.
33325943	14	42	dep	monitoring	2046:2055	arg1	i					2039:2039	i	2039:2039	i	2039:2039	For the future, N-glycan moieties on cancer senescent cells and their EV progeny hold potential for (i) the monitoring of treatment response as a liquid biopsy, and (ii) cancer senescent cell targeting with lectin therapies.
33325943	6	43	theme	TNBC	749:752	arg1	cells					754:758	the Cal51 TNBC cells	739:758	the Cal51 TNBC cells using the chemotherapeutic agent paclitaxel (PTX)	739:808	TIS was induced in the Cal51 TNBC cells using the chemotherapeutic agent paclitaxel (PTX).
33325943	10	44	theme	Protein	1255:1261	arg1	spectrometry					1268:1279	Protein mass spectrometry	1255:1279	Protein mass spectrometry	1255:1279	Protein mass spectrometry showed that the TIS cells contain different glycan modifying enzymes.
33325943	14	45	theme	cancer	1975:1980	arg1	cells					1992:1996	cancer senescent cells	1975:1996	cancer senescent cells	1975:1996	For the future, N-glycan moieties on cancer senescent cells and their EV progeny hold potential for (i) the monitoring of treatment response as a liquid biopsy, and (ii) cancer senescent cell targeting with lectin therapies.
33325943	11	46	theme	major	1608:1612	arg1	band					1621:1624	a major 32 kDa band	1606:1624	a major 32 kDa band only	1606:1629	The lectin, calnexin demonstrated a lower kDa size (∼58 kDa) in TIS compared to control cells (∼90 kDa) while Galectin 3 demonstrated potential proteolytic cleavage with 32 kDa and ∼22 kDa bands evident in TIS compared to non-senescent control cells with a major 32 kDa band only.
33325943	14	47	theme	cancer	2108:2113	arg1	targeting					2130:2138	(ii) cancer senescent cell targeting	2103:2138	(ii) cancer senescent cell targeting	2103:2138	For the future, N-glycan moieties on cancer senescent cells and their EV progeny hold potential for (i) the monitoring of treatment response as a liquid biopsy, and (ii) cancer senescent cell targeting with lectin therapies.
33325943	0	48	theme	therapeutic	35:45	arg1	TNBC					108:111	TNBC	108:111	TNBC	108:111	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.
33325943	0	48	theme	therapeutic	35:45	arg1	cells					101:105	therapeutic induced senescent (TIS) triple negative breast cancer cells	35:105	therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC)	35:112	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.
33325943	0	49	gly	glycosylation	9:21	arg1	TNBC					108:111	TNBC	108:111	TNBC	108:111	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.
33325943	0	49	gly	glycosylation	9:21	arg1	cells					101:105	therapeutic induced senescent (TIS) triple negative breast cancer cells	35:105	therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC)	35:112	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.
33325943	0	49	gly	glycosylation	9:21	arg1	progeny					151:157	their extracellular vesicle (EV) progeny	118:157	their extracellular vesicle (EV) progeny	118:157	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.
33325943	3	50	theme	Senescent	359:367	arg1	cells					369:373	Senescent cells	359:373	Senescent cells	359:373	Senescent cells release significantly more extracellular vesicles (EVs) than non-senescent cells.
33325943	0	51	theme	senescent	55:63	arg1	TNBC					108:111	TNBC	108:111	TNBC	108:111	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.
33325943	0	51	theme	senescent	55:63	arg1	cells					101:105	therapeutic induced senescent (TIS) triple negative breast cancer cells	35:105	therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC)	35:112	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.
33325943	10	52	contain	contain	1307:1313	arg1	cells					1301:1305	the TIS cells	1293:1305	the TIS cells	1293:1305	Protein mass spectrometry showed that the TIS cells contain different glycan modifying enzymes.
33325943	10	52	contain	contain	1307:1313	arg2	enzymes					1342:1348	different glycan modifying enzymes	1315:1348	different glycan modifying enzymes	1315:1348	Protein mass spectrometry showed that the TIS cells contain different glycan modifying enzymes.
33325943	8	53	theme	samples	1055:1061	arg1	blotting					1043:1050	lectin blotting	1036:1050	lectin blotting of samples	1036:1061	LC-Mass spectrometry (MS) analysis of the N-linked glycans and lectin blotting of samples was carried out to confirm the UPLC results.
33325943	8	53	theme	samples	1055:1061	arg1	analysis					999:1006	LC-Mass spectrometry (MS) analysis	973:1006	LC-Mass spectrometry (MS) analysis of the N-linked glycans	973:1030	LC-Mass spectrometry (MS) analysis of the N-linked glycans and lectin blotting of samples was carried out to confirm the UPLC results.
33325943	12	54	theme	non-senescent	1719:1731	arg1	cells					1733:1737	control non-senescent cells	1711:1737	control non-senescent cells	1711:1737	TIS CAL51 cells also demonstrated a reduced adhesion to collagen I compared to control non-senescent cells.
33325943	6	55	theme	Cal51	743:747	arg1	cells					754:758	the Cal51 TNBC cells	739:758	the Cal51 TNBC cells using the chemotherapeutic agent paclitaxel (PTX)	739:808	TIS was induced in the Cal51 TNBC cells using the chemotherapeutic agent paclitaxel (PTX).
33325943	7	56	theme	exoglycosidase	870:883	arg1	glycans					903:909	exoglycosidase digested N-linked glycans	870:909	exoglycosidase digested N-linked glycans	870:909	Ultra-performance liquid chromatography (UPLC) analysis of exoglycosidase digested N-linked glycans was carried out on TIS compared to non-treated control cells.
33325943	6	57	theme	agent	787:791	arg1	paclitaxel					793:802	the chemotherapeutic agent paclitaxel	766:802	the chemotherapeutic agent paclitaxel (PTX)	766:808	TIS was induced in the Cal51 TNBC cells using the chemotherapeutic agent paclitaxel (PTX).
33325943	6	57	theme	agent	787:791	arg1	PTX					805:807	PTX	805:807	PTX	805:807	TIS was induced in the Cal51 TNBC cells using the chemotherapeutic agent paclitaxel (PTX).
33325943	11	58	theme	kDa	1617:1619	arg1	band					1621:1624	a major 32 kDa band	1606:1624	a major 32 kDa band only	1606:1629	The lectin, calnexin demonstrated a lower kDa size (∼58 kDa) in TIS compared to control cells (∼90 kDa) while Galectin 3 demonstrated potential proteolytic cleavage with 32 kDa and ∼22 kDa bands evident in TIS compared to non-senescent control cells with a major 32 kDa band only.
33325943	11	59	theme	kDa	1536:1538	arg1	bands					1540:1544	∼22 kDa bands	1532:1544	∼22 kDa bands evident in TIS	1532:1559	The lectin, calnexin demonstrated a lower kDa size (∼58 kDa) in TIS compared to control cells (∼90 kDa) while Galectin 3 demonstrated potential proteolytic cleavage with 32 kDa and ∼22 kDa bands evident in TIS compared to non-senescent control cells with a major 32 kDa band only.
33325943	0	60	theme	progeny	151:157	arg1	profiles					23:30	N-Linked glycosylation profiles	0:30	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.	0:158	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.
33325943	10	61	theme	TIS	1297:1299	arg1	cells					1301:1305	the TIS cells	1293:1305	the TIS cells	1293:1305	Protein mass spectrometry showed that the TIS cells contain different glycan modifying enzymes.
33325943	8	62	link	N-linked	1015:1022	arg1	glycans					1024:1030	the N-linked glycans	1011:1030	the N-linked glycans	1011:1030	LC-Mass spectrometry (MS) analysis of the N-linked glycans and lectin blotting of samples was carried out to confirm the UPLC results.
33325943	1	63	theme	clinical	206:213	arg1	outcomes					215:222	poor clinical outcomes	201:222	poor clinical outcomes	201:222	Triple negative breast cancer (TNBC) has poor clinical outcomes and limited treatment options.
33325943	11	64	with	cleavage	1507:1514	arg1	kDa					1524:1526	32 kDa	1521:1526	32 kDa	1521:1526	The lectin, calnexin demonstrated a lower kDa size (∼58 kDa) in TIS compared to control cells (∼90 kDa) while Galectin 3 demonstrated potential proteolytic cleavage with 32 kDa and ∼22 kDa bands evident in TIS compared to non-senescent control cells with a major 32 kDa band only.
33325943	11	65	theme	evident	1546:1552	arg1	bands					1540:1544	∼22 kDa bands	1532:1544	∼22 kDa bands evident in TIS	1532:1559	The lectin, calnexin demonstrated a lower kDa size (∼58 kDa) in TIS compared to control cells (∼90 kDa) while Galectin 3 demonstrated potential proteolytic cleavage with 32 kDa and ∼22 kDa bands evident in TIS compared to non-senescent control cells with a major 32 kDa band only.
33325943	13	66	theme	non-senescent	1880:1892	arg1	cells					1900:1904	non-senescent Cal51 cells	1880:1904	non-senescent Cal51 cells	1880:1904	This study has shown that therapeutic-induced-senescent TNBC cells and their EV progeny, display differential N-glycan moieties compared to non-senescent Cal51 cells and their resultant EV progeny.
33325943	7	67	theme	chromatography	836:849	arg1	analysis					858:865	Ultra-performance liquid chromatography (UPLC) analysis	811:865	Ultra-performance liquid chromatography (UPLC) analysis of exoglycosidase digested N-linked glycans	811:909	Ultra-performance liquid chromatography (UPLC) analysis of exoglycosidase digested N-linked glycans was carried out on TIS compared to non-treated control cells.
33325943	12	68	theme	reduced	1668:1674	arg1	adhesion					1676:1683	a reduced adhesion	1666:1683	a reduced adhesion to collagen I compared to control non-senescent cells	1666:1737	TIS CAL51 cells also demonstrated a reduced adhesion to collagen I compared to control non-senescent cells.
33325943	14	69	theme	liquid	2084:2089	arg1	biopsy					2091:2096	a liquid biopsy	2082:2096	a liquid biopsy	2082:2096	For the future, N-glycan moieties on cancer senescent cells and their EV progeny hold potential for (i) the monitoring of treatment response as a liquid biopsy, and (ii) cancer senescent cell targeting with lectin therapies.
33325943	11	70	theme	control	1587:1593	arg1	cells					1595:1599	non-senescent control cells	1573:1599	non-senescent control cells with a major 32 kDa band only	1573:1629	The lectin, calnexin demonstrated a lower kDa size (∼58 kDa) in TIS compared to control cells (∼90 kDa) while Galectin 3 demonstrated potential proteolytic cleavage with 32 kDa and ∼22 kDa bands evident in TIS compared to non-senescent control cells with a major 32 kDa band only.
33325943	5	71	theme	cells	597:601	arg1	glycans					580:586	the N-linked glycans	567:586	the N-linked glycans of whole cells, membrane, cytoplasm and EVs harvested from TIS TNBC cells	567:660	We aimed to profile the N-linked glycans of whole cells, membrane, cytoplasm and EVs harvested from TIS TNBC cells and to compare these to results from non-senescent cells.
33325943	10	72	theme	glycan	1325:1330	arg1	enzymes					1342:1348	different glycan modifying enzymes	1315:1348	different glycan modifying enzymes	1315:1348	Protein mass spectrometry showed that the TIS cells contain different glycan modifying enzymes.
33325943	7	73	theme	Ultra-performance	811:827	arg1	UPLC					852:855	UPLC	852:855	UPLC	852:855	Ultra-performance liquid chromatography (UPLC) analysis of exoglycosidase digested N-linked glycans was carried out on TIS compared to non-treated control cells.
33325943	7	73	theme	Ultra-performance	811:827	arg1	chromatography					836:849	Ultra-performance liquid chromatography	811:849	Ultra-performance liquid chromatography (UPLC) analysis of exoglycosidase digested N-linked glycans	811:909	Ultra-performance liquid chromatography (UPLC) analysis of exoglycosidase digested N-linked glycans was carried out on TIS compared to non-treated control cells.
33325943	12	74	theme	TIS	1632:1634	arg1	cells					1642:1646	TIS CAL51 cells	1632:1646	TIS CAL51 cells	1632:1646	TIS CAL51 cells also demonstrated a reduced adhesion to collagen I compared to control non-senescent cells.
33325943	14	75	dep	targeting	2130:2138	arg1	ii					2104:2105	ii	2104:2105	ii	2104:2105	For the future, N-glycan moieties on cancer senescent cells and their EV progeny hold potential for (i) the monitoring of treatment response as a liquid biopsy, and (ii) cancer senescent cell targeting with lectin therapies.
33325943	11	76	theme	potential	1485:1493	arg1	cleavage					1507:1514	potential proteolytic cleavage	1485:1514	potential proteolytic cleavage with 32 kDa	1485:1526	The lectin, calnexin demonstrated a lower kDa size (∼58 kDa) in TIS compared to control cells (∼90 kDa) while Galectin 3 demonstrated potential proteolytic cleavage with 32 kDa and ∼22 kDa bands evident in TIS compared to non-senescent control cells with a major 32 kDa band only.
33325943	8	77	theme	spectrometry	981:992	arg1	analysis					999:1006	LC-Mass spectrometry (MS) analysis	973:1006	LC-Mass spectrometry (MS) analysis of the N-linked glycans	973:1030	LC-Mass spectrometry (MS) analysis of the N-linked glycans and lectin blotting of samples was carried out to confirm the UPLC results.
33325943	14	78	theme	treatment	2060:2068	arg1	response					2070:2077	treatment response	2060:2077	treatment response	2060:2077	For the future, N-glycan moieties on cancer senescent cells and their EV progeny hold potential for (i) the monitoring of treatment response as a liquid biopsy, and (ii) cancer senescent cell targeting with lectin therapies.
33325943	1	79	theme	negative	167:174	arg1	TNBC					191:194	TNBC	191:194	TNBC	191:194	Triple negative breast cancer (TNBC) has poor clinical outcomes and limited treatment options.
33325943	1	79	theme	negative	167:174	arg1	cancer					183:188	Triple negative breast cancer	160:188	Triple negative breast cancer (TNBC)	160:195	Triple negative breast cancer (TNBC) has poor clinical outcomes and limited treatment options.
33325943	9	80	theme	membrane	1180:1187	arg1	profile					1159:1165	the N-glycan profile	1146:1165	the N-glycan profile of the Cal51 membrane, cytoplasm and EV progeny of TIS compared to non-senescent cells	1146:1252	Significant differences were found in the N-glycan profile of the Cal51 membrane, cytoplasm and EV progeny of TIS compared to non-senescent cells.
33325943	11	81	dep	demonstrated	1372:1383	arg1	demonstrated					1472:1483	demonstrated	1472:1483	demonstrated a lower kDa size (∼58 kDa) in TIS compared to control cells (∼90 kDa) while Galectin 3 demonstrated potential proteolytic cleavage with 32 kDa and ∼22 kDa bands evident in TIS compared to non-senescent control cells with a major 32 kDa band only	1372:1629	The lectin, calnexin demonstrated a lower kDa size (∼58 kDa) in TIS compared to control cells (∼90 kDa) while Galectin 3 demonstrated potential proteolytic cleavage with 32 kDa and ∼22 kDa bands evident in TIS compared to non-senescent control cells with a major 32 kDa band only.
33325943	0	82	theme	vesicle	138:144	arg1	progeny					151:157	their extracellular vesicle (EV) progeny	118:157	their extracellular vesicle (EV) progeny	118:157	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.
33325943	9	83	theme	cytoplasm	1190:1198	arg1	profile					1159:1165	the N-glycan profile	1146:1165	the N-glycan profile of the Cal51 membrane, cytoplasm and EV progeny of TIS compared to non-senescent cells	1146:1252	Significant differences were found in the N-glycan profile of the Cal51 membrane, cytoplasm and EV progeny of TIS compared to non-senescent cells.
33325943	13	84	theme	EV	1817:1818	arg1	progeny					1820:1826	their EV progeny	1811:1826	their EV progeny	1811:1826	This study has shown that therapeutic-induced-senescent TNBC cells and their EV progeny, display differential N-glycan moieties compared to non-senescent Cal51 cells and their resultant EV progeny.
33325943	12	85	theme	CAL51	1636:1640	arg1	cells					1642:1646	TIS CAL51 cells	1632:1646	TIS CAL51 cells	1632:1646	TIS CAL51 cells also demonstrated a reduced adhesion to collagen I compared to control non-senescent cells.
33325943	5	86	theme	TNBC	651:654	arg1	cells					656:660	TIS TNBC cells	647:660	TIS TNBC cells	647:660	We aimed to profile the N-linked glycans of whole cells, membrane, cytoplasm and EVs harvested from TIS TNBC cells and to compare these to results from non-senescent cells.
33325943	9	87	theme	EV	1204:1205	arg1	progeny					1207:1213	EV progeny	1204:1213	EV progeny	1204:1213	Significant differences were found in the N-glycan profile of the Cal51 membrane, cytoplasm and EV progeny of TIS compared to non-senescent cells.
33325943	7	88	link	N-linked	894:901	arg1	glycans					903:909	exoglycosidase digested N-linked glycans	870:909	exoglycosidase digested N-linked glycans	870:909	Ultra-performance liquid chromatography (UPLC) analysis of exoglycosidase digested N-linked glycans was carried out on TIS compared to non-treated control cells.
33325943	7	89	theme	control	958:964	arg1	cells					966:970	non-treated control cells	946:970	non-treated control cells	946:970	Ultra-performance liquid chromatography (UPLC) analysis of exoglycosidase digested N-linked glycans was carried out on TIS compared to non-treated control cells.
33325943	13	90	theme	differential	1837:1848	arg1	moieties					1859:1866	differential N-glycan moieties	1837:1866	differential N-glycan moieties	1837:1866	This study has shown that therapeutic-induced-senescent TNBC cells and their EV progeny, display differential N-glycan moieties compared to non-senescent Cal51 cells and their resultant EV progeny.
33325943	13	91	theme	TNBC	1796:1799	arg1	cells					1801:1805	therapeutic-induced-senescent TNBC cells	1766:1805	therapeutic-induced-senescent TNBC cells	1766:1805	This study has shown that therapeutic-induced-senescent TNBC cells and their EV progeny, display differential N-glycan moieties compared to non-senescent Cal51 cells and their resultant EV progeny.
33325943	0	92	theme	negative	78:85	arg1	TNBC					108:111	TNBC	108:111	TNBC	108:111	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.
33325943	0	92	theme	negative	78:85	arg1	cells					101:105	therapeutic induced senescent (TIS) triple negative breast cancer cells	35:105	therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC)	35:112	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.
33325943	4	93	theme	glycosylation	483:495	arg1	alterations					497:507	N- and O-linked glycosylation alterations	467:507	N- and O-linked glycosylation alterations	467:507	Recently, N- and O-linked glycosylation alterations have been associated with senescence.
33325943	14	94	from	moieties	1963:1970	arg1	progeny					2011:2017	their EV progeny	2002:2017	their EV progeny	2002:2017	For the future, N-glycan moieties on cancer senescent cells and their EV progeny hold potential for (i) the monitoring of treatment response as a liquid biopsy, and (ii) cancer senescent cell targeting with lectin therapies.
33325943	14	94	from	moieties	1963:1970	arg1	cells					1992:1996	cancer senescent cells	1975:1996	cancer senescent cells	1975:1996	For the future, N-glycan moieties on cancer senescent cells and their EV progeny hold potential for (i) the monitoring of treatment response as a liquid biopsy, and (ii) cancer senescent cell targeting with lectin therapies.
33325943	0	95	theme	cancer	94:99	arg1	TNBC					108:111	TNBC	108:111	TNBC	108:111	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.
33325943	0	95	theme	cancer	94:99	arg1	cells					101:105	therapeutic induced senescent (TIS) triple negative breast cancer cells	35:105	therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC)	35:112	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.
33325943	9	96	theme	non-senescent	1234:1246	arg1	cells					1248:1252	non-senescent cells	1234:1252	non-senescent cells	1234:1252	Significant differences were found in the N-glycan profile of the Cal51 membrane, cytoplasm and EV progeny of TIS compared to non-senescent cells.
33325943	2	97	dep	result	306:311	arg1	killing					275:281	killing	275:281	killing some cancer cells	275:299	Chemotherapy, while killing some cancer cells, can result in therapeutic-induced-senescent (TIS) cells.
33325943	11	98	theme	lower	1387:1391	arg1	size					1397:1400	a lower kDa size	1385:1400	a lower kDa size (∼58 kDa) in TIS compared to control cells (∼90 kDa) while Galectin 3	1385:1470	The lectin, calnexin demonstrated a lower kDa size (∼58 kDa) in TIS compared to control cells (∼90 kDa) while Galectin 3 demonstrated potential proteolytic cleavage with 32 kDa and ∼22 kDa bands evident in TIS compared to non-senescent control cells with a major 32 kDa band only.
33325943	11	98	theme	lower	1387:1391	arg1	kDa					1407:1409	∼58 kDa	1403:1409	∼58 kDa	1403:1409	The lectin, calnexin demonstrated a lower kDa size (∼58 kDa) in TIS compared to control cells (∼90 kDa) while Galectin 3 demonstrated potential proteolytic cleavage with 32 kDa and ∼22 kDa bands evident in TIS compared to non-senescent control cells with a major 32 kDa band only.
33325943	5	99	theme	whole	591:595	arg1	membrane					604:611	membrane	604:611	membrane	604:611	We aimed to profile the N-linked glycans of whole cells, membrane, cytoplasm and EVs harvested from TIS TNBC cells and to compare these to results from non-senescent cells.
33325943	5	99	theme	whole	591:595	arg1	cells					597:601	whole cells	591:601	whole cells	591:601	We aimed to profile the N-linked glycans of whole cells, membrane, cytoplasm and EVs harvested from TIS TNBC cells and to compare these to results from non-senescent cells.
33325943	5	99	theme	whole	591:595	arg1	EVs					628:630	EVs	628:630	EVs	628:630	We aimed to profile the N-linked glycans of whole cells, membrane, cytoplasm and EVs harvested from TIS TNBC cells and to compare these to results from non-senescent cells.
33325943	5	99	theme	whole	591:595	arg1	cytoplasm					614:622	cytoplasm	614:622	cytoplasm	614:622	We aimed to profile the N-linked glycans of whole cells, membrane, cytoplasm and EVs harvested from TIS TNBC cells and to compare these to results from non-senescent cells.
33325943	14	100	theme	EV	2008:2009	arg1	progeny					2011:2017	their EV progeny	2002:2017	their EV progeny	2002:2017	For the future, N-glycan moieties on cancer senescent cells and their EV progeny hold potential for (i) the monitoring of treatment response as a liquid biopsy, and (ii) cancer senescent cell targeting with lectin therapies.
33325943	14	101	theme	lectin	2145:2150	arg1	therapies					2152:2160	lectin therapies	2145:2160	lectin therapies	2145:2160	For the future, N-glycan moieties on cancer senescent cells and their EV progeny hold potential for (i) the monitoring of treatment response as a liquid biopsy, and (ii) cancer senescent cell targeting with lectin therapies.
33325943	0	102	theme	glycosylation	9:21	arg1	profiles					23:30	N-Linked glycosylation profiles	0:30	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.	0:158	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.
33325943	8	103	theme	glycans	1024:1030	arg1	blotting					1043:1050	lectin blotting	1036:1050	lectin blotting of samples	1036:1061	LC-Mass spectrometry (MS) analysis of the N-linked glycans and lectin blotting of samples was carried out to confirm the UPLC results.
33325943	8	103	theme	glycans	1024:1030	arg1	analysis					999:1006	LC-Mass spectrometry (MS) analysis	973:1006	LC-Mass spectrometry (MS) analysis of the N-linked glycans	973:1030	LC-Mass spectrometry (MS) analysis of the N-linked glycans and lectin blotting of samples was carried out to confirm the UPLC results.
33325943	10	104	theme	mass	1263:1266	arg1	spectrometry					1268:1279	Protein mass spectrometry	1255:1279	Protein mass spectrometry	1255:1279	Protein mass spectrometry showed that the TIS cells contain different glycan modifying enzymes.
33325943	3	105	theme	extracellular	402:414	arg1	vesicles					416:423	significantly more extracellular vesicles	383:423	significantly more extracellular vesicles (EVs)	383:429	Senescent cells release significantly more extracellular vesicles (EVs) than non-senescent cells.
33325943	3	105	theme	extracellular	402:414	arg1	EVs					426:428	EVs	426:428	EVs	426:428	Senescent cells release significantly more extracellular vesicles (EVs) than non-senescent cells.
33325943	8	106	theme	lectin	1036:1041	arg1	blotting					1043:1050	lectin blotting	1036:1050	lectin blotting of samples	1036:1061	LC-Mass spectrometry (MS) analysis of the N-linked glycans and lectin blotting of samples was carried out to confirm the UPLC results.
33325943	7	107	theme	N-linked	894:901	arg1	glycans					903:909	exoglycosidase digested N-linked glycans	870:909	exoglycosidase digested N-linked glycans	870:909	Ultra-performance liquid chromatography (UPLC) analysis of exoglycosidase digested N-linked glycans was carried out on TIS compared to non-treated control cells.
33325943	14	108	theme	senescent	1982:1990	arg1	cells					1992:1996	cancer senescent cells	1975:1996	cancer senescent cells	1975:1996	For the future, N-glycan moieties on cancer senescent cells and their EV progeny hold potential for (i) the monitoring of treatment response as a liquid biopsy, and (ii) cancer senescent cell targeting with lectin therapies.
33325943	14	109	theme	senescent	2115:2123	arg1	targeting					2130:2138	(ii) cancer senescent cell targeting	2103:2138	(ii) cancer senescent cell targeting	2103:2138	For the future, N-glycan moieties on cancer senescent cells and their EV progeny hold potential for (i) the monitoring of treatment response as a liquid biopsy, and (ii) cancer senescent cell targeting with lectin therapies.
33325943	3	110	theme	non-senescent	436:448	arg1	cells					450:454	non-senescent cells	436:454	non-senescent cells	436:454	Senescent cells release significantly more extracellular vesicles (EVs) than non-senescent cells.
33325943	0	111	theme	induced	47:53	arg1	TNBC					108:111	TNBC	108:111	TNBC	108:111	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.
33325943	0	111	theme	induced	47:53	arg1	cells					101:105	therapeutic induced senescent (TIS) triple negative breast cancer cells	35:105	therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC)	35:112	N-Linked glycosylation profiles of therapeutic induced senescent (TIS) triple negative breast cancer cells (TNBC) and their extracellular vesicle (EV) progeny.
33325943	11	112	from	size	1397:1400	arg1	TIS					1415:1417	TIS	1415:1417	TIS compared to control cells (∼90 kDa) while Galectin 3	1415:1470	The lectin, calnexin demonstrated a lower kDa size (∼58 kDa) in TIS compared to control cells (∼90 kDa) while Galectin 3 demonstrated potential proteolytic cleavage with 32 kDa and ∼22 kDa bands evident in TIS compared to non-senescent control cells with a major 32 kDa band only.
33325943	11	113	from	evident	1546:1552	arg1	TIS					1557:1559	TIS	1557:1559	TIS	1557:1559	The lectin, calnexin demonstrated a lower kDa size (∼58 kDa) in TIS compared to control cells (∼90 kDa) while Galectin 3 demonstrated potential proteolytic cleavage with 32 kDa and ∼22 kDa bands evident in TIS compared to non-senescent control cells with a major 32 kDa band only.
33325943	14	114	with	potential	2024:2032	arg1	therapies					2152:2160	lectin therapies	2145:2160	lectin therapies	2145:2160	For the future, N-glycan moieties on cancer senescent cells and their EV progeny hold potential for (i) the monitoring of treatment response as a liquid biopsy, and (ii) cancer senescent cell targeting with lectin therapies.
33325943	5	115	theme	non-senescent	699:711	arg1	cells					713:717	non-senescent cells	699:717	non-senescent cells	699:717	We aimed to profile the N-linked glycans of whole cells, membrane, cytoplasm and EVs harvested from TIS TNBC cells and to compare these to results from non-senescent cells.
33325943	11	116	from	TIS	1557:1559	arg1	evident					1546:1552	evident	1546:1552	evident	1546:1552	The lectin, calnexin demonstrated a lower kDa size (∼58 kDa) in TIS compared to control cells (∼90 kDa) while Galectin 3 demonstrated potential proteolytic cleavage with 32 kDa and ∼22 kDa bands evident in TIS compared to non-senescent control cells with a major 32 kDa band only.
33325943	14	117	theme	N-glycan	1954:1961	arg1	moieties					1963:1970	N-glycan moieties	1954:1970	N-glycan moieties on cancer senescent cells and their EV progeny	1954:2017	For the future, N-glycan moieties on cancer senescent cells and their EV progeny hold potential for (i) the monitoring of treatment response as a liquid biopsy, and (ii) cancer senescent cell targeting with lectin therapies.
33325943	11	118	theme	proteolytic	1495:1505	arg1	cleavage					1507:1514	potential proteolytic cleavage	1485:1514	potential proteolytic cleavage with 32 kDa	1485:1526	The lectin, calnexin demonstrated a lower kDa size (∼58 kDa) in TIS compared to control cells (∼90 kDa) while Galectin 3 demonstrated potential proteolytic cleavage with 32 kDa and ∼22 kDa bands evident in TIS compared to non-senescent control cells with a major 32 kDa band only.
33325943	12	119	theme	control	1711:1717	arg1	cells					1733:1737	control non-senescent cells	1711:1737	control non-senescent cells	1711:1737	TIS CAL51 cells also demonstrated a reduced adhesion to collagen I compared to control non-senescent cells.
33325943	13	120	theme	resultant	1916:1924	arg1	progeny					1929:1935	their resultant EV progeny	1910:1935	their resultant EV progeny	1910:1935	This study has shown that therapeutic-induced-senescent TNBC cells and their EV progeny, display differential N-glycan moieties compared to non-senescent Cal51 cells and their resultant EV progeny.
33325943	10	121	theme	different	1315:1323	arg1	enzymes					1342:1348	different glycan modifying enzymes	1315:1348	different glycan modifying enzymes	1315:1348	Protein mass spectrometry showed that the TIS cells contain different glycan modifying enzymes.
33325943	2	122	theme	cancer	288:293	arg1	cells					295:299	some cancer cells	283:299	some cancer cells	283:299	Chemotherapy, while killing some cancer cells, can result in therapeutic-induced-senescent (TIS) cells.
33325943	6	123	theme	chemotherapeutic	770:785	arg1	paclitaxel					793:802	the chemotherapeutic agent paclitaxel	766:802	the chemotherapeutic agent paclitaxel (PTX)	766:808	TIS was induced in the Cal51 TNBC cells using the chemotherapeutic agent paclitaxel (PTX).
33325943	6	123	theme	chemotherapeutic	770:785	arg1	PTX					805:807	PTX	805:807	PTX	805:807	TIS was induced in the Cal51 TNBC cells using the chemotherapeutic agent paclitaxel (PTX).
33325943	9	124	theme	Significant	1108:1118	arg1	differences					1120:1130	Significant differences	1108:1130	Significant differences	1108:1130	Significant differences were found in the N-glycan profile of the Cal51 membrane, cytoplasm and EV progeny of TIS compared to non-senescent cells.
33325943	2	125	theme	therapeutic-induced-senescent	316:344	arg1	cells					352:356	therapeutic-induced-senescent (TIS) cells	316:356	therapeutic-induced-senescent (TIS) cells	316:356	Chemotherapy, while killing some cancer cells, can result in therapeutic-induced-senescent (TIS) cells.
33325943	2	125	theme	therapeutic-induced-senescent	316:344	arg1	TIS					347:349	TIS	347:349	TIS	347:349	Chemotherapy, while killing some cancer cells, can result in therapeutic-induced-senescent (TIS) cells.
33325943	11	126	with	bands	1540:1544	arg1	kDa					1524:1526	32 kDa	1521:1526	32 kDa	1521:1526	The lectin, calnexin demonstrated a lower kDa size (∼58 kDa) in TIS compared to control cells (∼90 kDa) while Galectin 3 demonstrated potential proteolytic cleavage with 32 kDa and ∼22 kDa bands evident in TIS compared to non-senescent control cells with a major 32 kDa band only.
33325943	1	127	theme	poor	201:204	arg1	outcomes					215:222	poor clinical outcomes	201:222	poor clinical outcomes	201:222	Triple negative breast cancer (TNBC) has poor clinical outcomes and limited treatment options.
33069824	3	0	with	favorable	854:862	arg1	chemisorption					933:945	chemisorption	933:945	chemisorption	933:945	The adsorptive removal of lead ions by the nanocomposite was investigated through batch experiments conducted under different pH, contact times and Pb(II) initial concentrations, proving that the process was highly favorable according to the Langmuir isotherm model (monolayer adsorption) with chemisorption as the main mechanism and kinetic data obeying a nonlinear pseudo-second order kinetic model.
33069824	1	1	theme	renewable	176:184	arg1	nanofibrils					225:235	bacterial cellulose (BC) nanofibrils	200:235	bacterial cellulose (BC) nanofibrils	200:235	An environmentally friendly nanocomposite adsorbent composed of two renewable biomaterials, bacterial cellulose (BC) nanofibrils and hydroxyapatite (HA) nanocrystals, was synthetized by an in situ wet chemical precipitation technique, using clam shell biowaste as feedstock.
33069824	1	1	theme	renewable	176:184	arg1	nanocrystals					261:272	hydroxyapatite (HA) nanocrystals	241:272	hydroxyapatite (HA) nanocrystals	241:272	An environmentally friendly nanocomposite adsorbent composed of two renewable biomaterials, bacterial cellulose (BC) nanofibrils and hydroxyapatite (HA) nanocrystals, was synthetized by an in situ wet chemical precipitation technique, using clam shell biowaste as feedstock.
33069824	1	1	theme	renewable	176:184	arg1	biomaterials					186:197	two renewable biomaterials	172:197	two renewable biomaterials	172:197	An environmentally friendly nanocomposite adsorbent composed of two renewable biomaterials, bacterial cellulose (BC) nanofibrils and hydroxyapatite (HA) nanocrystals, was synthetized by an in situ wet chemical precipitation technique, using clam shell biowaste as feedstock.
33069824	3	2	dep	pH	765:766	arg1	concentrations					802:815	initial concentrations	794:815	initial concentrations	794:815	The adsorptive removal of lead ions by the nanocomposite was investigated through batch experiments conducted under different pH, contact times and Pb(II) initial concentrations, proving that the process was highly favorable according to the Langmuir isotherm model (monolayer adsorption) with chemisorption as the main mechanism and kinetic data obeying a nonlinear pseudo-second order kinetic model.
33069824	1	3	theme	clam	349:352	arg1	biowaste					360:367	clam shell biowaste	349:367	clam shell biowaste	349:367	An environmentally friendly nanocomposite adsorbent composed of two renewable biomaterials, bacterial cellulose (BC) nanofibrils and hydroxyapatite (HA) nanocrystals, was synthetized by an in situ wet chemical precipitation technique, using clam shell biowaste as feedstock.
33069824	1	4	theme	shell	354:358	arg1	biowaste					360:367	clam shell biowaste	349:367	clam shell biowaste	349:367	An environmentally friendly nanocomposite adsorbent composed of two renewable biomaterials, bacterial cellulose (BC) nanofibrils and hydroxyapatite (HA) nanocrystals, was synthetized by an in situ wet chemical precipitation technique, using clam shell biowaste as feedstock.
33069824	2	5	dep	techniques	498:507	arg1	XRD					521:523	XRD	521:523	XRD	521:523	HA nanocrystals embedded in an ultrafine BC network were confirmed and characterized trough different instrumental techniques (SEM, FTIR, XRD, EDS, surface charge and BET analysis), describing its nanostructure, chemical composition and thermal stability.
33069824	2	5	dep	techniques	498:507	arg1	EDS					526:528	EDS	526:528	EDS	526:528	HA nanocrystals embedded in an ultrafine BC network were confirmed and characterized trough different instrumental techniques (SEM, FTIR, XRD, EDS, surface charge and BET analysis), describing its nanostructure, chemical composition and thermal stability.
33069824	2	5	dep	techniques	498:507	arg1	analysis					554:561	BET analysis	550:561	BET analysis	550:561	HA nanocrystals embedded in an ultrafine BC network were confirmed and characterized trough different instrumental techniques (SEM, FTIR, XRD, EDS, surface charge and BET analysis), describing its nanostructure, chemical composition and thermal stability.
33069824	2	5	dep	techniques	498:507	arg1	SEM					510:512	SEM	510:512	SEM	510:512	HA nanocrystals embedded in an ultrafine BC network were confirmed and characterized trough different instrumental techniques (SEM, FTIR, XRD, EDS, surface charge and BET analysis), describing its nanostructure, chemical composition and thermal stability.
33069824	2	5	dep	techniques	498:507	arg1	FTIR					515:518	FTIR	515:518	FTIR	515:518	HA nanocrystals embedded in an ultrafine BC network were confirmed and characterized trough different instrumental techniques (SEM, FTIR, XRD, EDS, surface charge and BET analysis), describing its nanostructure, chemical composition and thermal stability.
33069824	2	5	dep	techniques	498:507	arg1	charge					539:544	surface charge	531:544	surface charge	531:544	HA nanocrystals embedded in an ultrafine BC network were confirmed and characterized trough different instrumental techniques (SEM, FTIR, XRD, EDS, surface charge and BET analysis), describing its nanostructure, chemical composition and thermal stability.
33069824	3	6	theme	nonlinear	996:1004	arg1	model					1034:1038	a nonlinear pseudo-second order kinetic model	994:1038	a nonlinear pseudo-second order kinetic model	994:1038	The adsorptive removal of lead ions by the nanocomposite was investigated through batch experiments conducted under different pH, contact times and Pb(II) initial concentrations, proving that the process was highly favorable according to the Langmuir isotherm model (monolayer adsorption) with chemisorption as the main mechanism and kinetic data obeying a nonlinear pseudo-second order kinetic model.
33069824	4	7	theme	developed	1045:1053	arg1	nanocomposite					1055:1067	The developed nanocomposite	1041:1067	The developed nanocomposite	1041:1067	The developed nanocomposite showed a strong removal capacity of Pb(II) both in batch experiments (192 mg/g) and packed-bed column systems (188 mg/g), placing this new nanocomposite among top-performing BC-based biomaterials for lead removal.
33069824	3	8	theme	initial	794:800	arg1	concentrations					802:815	initial concentrations	794:815	initial concentrations	794:815	The adsorptive removal of lead ions by the nanocomposite was investigated through batch experiments conducted under different pH, contact times and Pb(II) initial concentrations, proving that the process was highly favorable according to the Langmuir isotherm model (monolayer adsorption) with chemisorption as the main mechanism and kinetic data obeying a nonlinear pseudo-second order kinetic model.
33069824	4	9	theme	packed-bed	1153:1162	arg1	188 mg/g					1180:1187	188 mg/g	1180:1187	188 mg/g	1180:1187	The developed nanocomposite showed a strong removal capacity of Pb(II) both in batch experiments (192 mg/g) and packed-bed column systems (188 mg/g), placing this new nanocomposite among top-performing BC-based biomaterials for lead removal.
33069824	4	9	theme	packed-bed	1153:1162	arg1	systems					1171:1177	packed-bed column systems	1153:1177	packed-bed column systems (188 mg/g)	1153:1188	The developed nanocomposite showed a strong removal capacity of Pb(II) both in batch experiments (192 mg/g) and packed-bed column systems (188 mg/g), placing this new nanocomposite among top-performing BC-based biomaterials for lead removal.
33069824	3	10	theme	batch	721:725	arg1	experiments					727:737	batch experiments	721:737	batch experiments conducted under different pH, contact times and Pb(II) initial concentrations	721:815	The adsorptive removal of lead ions by the nanocomposite was investigated through batch experiments conducted under different pH, contact times and Pb(II) initial concentrations, proving that the process was highly favorable according to the Langmuir isotherm model (monolayer adsorption) with chemisorption as the main mechanism and kinetic data obeying a nonlinear pseudo-second order kinetic model.
33069824	4	11	theme	new	1204:1206	arg1	nanocomposite					1208:1220	this new nanocomposite	1199:1220	this new nanocomposite	1199:1220	The developed nanocomposite showed a strong removal capacity of Pb(II) both in batch experiments (192 mg/g) and packed-bed column systems (188 mg/g), placing this new nanocomposite among top-performing BC-based biomaterials for lead removal.
33069824	3	12	theme	isotherm	890:897	arg1	adsorption					916:925	the Langmuir isotherm model (monolayer adsorption)	877:926	the Langmuir isotherm model (monolayer adsorption)	877:926	The adsorptive removal of lead ions by the nanocomposite was investigated through batch experiments conducted under different pH, contact times and Pb(II) initial concentrations, proving that the process was highly favorable according to the Langmuir isotherm model (monolayer adsorption) with chemisorption as the main mechanism and kinetic data obeying a nonlinear pseudo-second order kinetic model.
33069824	2	13	theme	thermal	620:626	arg1	stability					628:636	thermal stability	620:636	thermal stability	620:636	HA nanocrystals embedded in an ultrafine BC network were confirmed and characterized trough different instrumental techniques (SEM, FTIR, XRD, EDS, surface charge and BET analysis), describing its nanostructure, chemical composition and thermal stability.
33069824	3	14	theme	model	899:903	arg1	adsorption					916:925	the Langmuir isotherm model (monolayer adsorption)	877:926	the Langmuir isotherm model (monolayer adsorption)	877:926	The adsorptive removal of lead ions by the nanocomposite was investigated through batch experiments conducted under different pH, contact times and Pb(II) initial concentrations, proving that the process was highly favorable according to the Langmuir isotherm model (monolayer adsorption) with chemisorption as the main mechanism and kinetic data obeying a nonlinear pseudo-second order kinetic model.
33069824	3	15	theme	kinetic	1026:1032	arg1	model					1034:1038	a nonlinear pseudo-second order kinetic model	994:1038	a nonlinear pseudo-second order kinetic model	994:1038	The adsorptive removal of lead ions by the nanocomposite was investigated through batch experiments conducted under different pH, contact times and Pb(II) initial concentrations, proving that the process was highly favorable according to the Langmuir isotherm model (monolayer adsorption) with chemisorption as the main mechanism and kinetic data obeying a nonlinear pseudo-second order kinetic model.
33069824	1	16	theme	bacterial	200:208	arg1	cellulose					210:218	bacterial cellulose	200:218	bacterial cellulose (BC) nanofibrils	200:235	An environmentally friendly nanocomposite adsorbent composed of two renewable biomaterials, bacterial cellulose (BC) nanofibrils and hydroxyapatite (HA) nanocrystals, was synthetized by an in situ wet chemical precipitation technique, using clam shell biowaste as feedstock.
33069824	1	16	theme	bacterial	200:208	arg1	BC					221:222	BC	221:222	BC	221:222	An environmentally friendly nanocomposite adsorbent composed of two renewable biomaterials, bacterial cellulose (BC) nanofibrils and hydroxyapatite (HA) nanocrystals, was synthetized by an in situ wet chemical precipitation technique, using clam shell biowaste as feedstock.
33069824	0	17	theme	ecofriendly	3:13	arg1	nanocomposite					15:27	An ecofriendly nanocomposite	0:27	An ecofriendly nanocomposite of bacterial cellulose and hydroxyapatite	0:69	An ecofriendly nanocomposite of bacterial cellulose and hydroxyapatite efficiently removes lead from water.
33069824	0	17	theme	ecofriendly	3:13	arg1	cellulose					42:50	bacterial cellulose	32:50	bacterial cellulose	32:50	An ecofriendly nanocomposite of bacterial cellulose and hydroxyapatite efficiently removes lead from water.
33069824	0	17	theme	ecofriendly	3:13	arg1	hydroxyapatite					56:69	hydroxyapatite	56:69	hydroxyapatite	56:69	An ecofriendly nanocomposite of bacterial cellulose and hydroxyapatite efficiently removes lead from water.
33069824	3	18	theme	Langmuir	881:888	arg1	adsorption					916:925	the Langmuir isotherm model (monolayer adsorption)	877:926	the Langmuir isotherm model (monolayer adsorption)	877:926	The adsorptive removal of lead ions by the nanocomposite was investigated through batch experiments conducted under different pH, contact times and Pb(II) initial concentrations, proving that the process was highly favorable according to the Langmuir isotherm model (monolayer adsorption) with chemisorption as the main mechanism and kinetic data obeying a nonlinear pseudo-second order kinetic model.
33069824	1	19	theme	cellulose	210:218	arg1	nanofibrils					225:235	bacterial cellulose (BC) nanofibrils	200:235	bacterial cellulose (BC) nanofibrils	200:235	An environmentally friendly nanocomposite adsorbent composed of two renewable biomaterials, bacterial cellulose (BC) nanofibrils and hydroxyapatite (HA) nanocrystals, was synthetized by an in situ wet chemical precipitation technique, using clam shell biowaste as feedstock.
33069824	1	19	theme	cellulose	210:218	arg1	biomaterials					186:197	two renewable biomaterials	172:197	two renewable biomaterials	172:197	An environmentally friendly nanocomposite adsorbent composed of two renewable biomaterials, bacterial cellulose (BC) nanofibrils and hydroxyapatite (HA) nanocrystals, was synthetized by an in situ wet chemical precipitation technique, using clam shell biowaste as feedstock.
33069824	3	20	theme	different	755:763	arg1	pH					765:766	different pH	755:766	different pH	755:766	The adsorptive removal of lead ions by the nanocomposite was investigated through batch experiments conducted under different pH, contact times and Pb(II) initial concentrations, proving that the process was highly favorable according to the Langmuir isotherm model (monolayer adsorption) with chemisorption as the main mechanism and kinetic data obeying a nonlinear pseudo-second order kinetic model.
33069824	2	21	theme	chemical	595:602	arg1	composition					604:614	chemical composition	595:614	chemical composition	595:614	HA nanocrystals embedded in an ultrafine BC network were confirmed and characterized trough different instrumental techniques (SEM, FTIR, XRD, EDS, surface charge and BET analysis), describing its nanostructure, chemical composition and thermal stability.
33069824	3	22	theme	pseudo-second	1006:1018	arg1	model					1034:1038	a nonlinear pseudo-second order kinetic model	994:1038	a nonlinear pseudo-second order kinetic model	994:1038	The adsorptive removal of lead ions by the nanocomposite was investigated through batch experiments conducted under different pH, contact times and Pb(II) initial concentrations, proving that the process was highly favorable according to the Langmuir isotherm model (monolayer adsorption) with chemisorption as the main mechanism and kinetic data obeying a nonlinear pseudo-second order kinetic model.
33069824	2	23	theme	HA	383:384	arg1	nanocrystals					386:397	HA nanocrystals	383:397	HA nanocrystals embedded in an ultrafine BC network	383:433	HA nanocrystals embedded in an ultrafine BC network were confirmed and characterized trough different instrumental techniques (SEM, FTIR, XRD, EDS, surface charge and BET analysis), describing its nanostructure, chemical composition and thermal stability.
33069824	3	24	theme	order	1020:1024	arg1	model					1034:1038	a nonlinear pseudo-second order kinetic model	994:1038	a nonlinear pseudo-second order kinetic model	994:1038	The adsorptive removal of lead ions by the nanocomposite was investigated through batch experiments conducted under different pH, contact times and Pb(II) initial concentrations, proving that the process was highly favorable according to the Langmuir isotherm model (monolayer adsorption) with chemisorption as the main mechanism and kinetic data obeying a nonlinear pseudo-second order kinetic model.
33069824	0	25	dep	removes	83:89	arg1	lead					91:94	lead	91:94	removes lead from water	83:105	An ecofriendly nanocomposite of bacterial cellulose and hydroxyapatite efficiently removes lead from water.
33069824	3	26	dep	mechanism	959:967	arg1	the					950:952	the	950:952	the	950:952	The adsorptive removal of lead ions by the nanocomposite was investigated through batch experiments conducted under different pH, contact times and Pb(II) initial concentrations, proving that the process was highly favorable according to the Langmuir isotherm model (monolayer adsorption) with chemisorption as the main mechanism and kinetic data obeying a nonlinear pseudo-second order kinetic model.
33069824	4	27	theme	batch	1120:1124	arg1	192 mg/g					1139:1146	192 mg/g	1139:1146	192 mg/g	1139:1146	The developed nanocomposite showed a strong removal capacity of Pb(II) both in batch experiments (192 mg/g) and packed-bed column systems (188 mg/g), placing this new nanocomposite among top-performing BC-based biomaterials for lead removal.
33069824	4	27	theme	batch	1120:1124	arg1	experiments					1126:1136	batch experiments	1120:1136	batch experiments (192 mg/g)	1120:1147	The developed nanocomposite showed a strong removal capacity of Pb(II) both in batch experiments (192 mg/g) and packed-bed column systems (188 mg/g), placing this new nanocomposite among top-performing BC-based biomaterials for lead removal.
33069824	1	28	theme	in	297:298	arg1	technique					332:340	an in situ wet chemical precipitation technique	294:340	an in situ wet chemical precipitation technique	294:340	An environmentally friendly nanocomposite adsorbent composed of two renewable biomaterials, bacterial cellulose (BC) nanofibrils and hydroxyapatite (HA) nanocrystals, was synthetized by an in situ wet chemical precipitation technique, using clam shell biowaste as feedstock.
33069824	1	29	theme	environmentally	111:125	arg1	nanocomposite					136:148	An environmentally friendly nanocomposite adsorbent	108:158	An environmentally friendly nanocomposite adsorbent composed of two renewable biomaterials, bacterial cellulose (BC) nanofibrils and hydroxyapatite (HA) nanocrystals,	108:273	An environmentally friendly nanocomposite adsorbent composed of two renewable biomaterials, bacterial cellulose (BC) nanofibrils and hydroxyapatite (HA) nanocrystals, was synthetized by an in situ wet chemical precipitation technique, using clam shell biowaste as feedstock.
33069824	2	30	theme	BC	424:425	arg1	network					427:433	an ultrafine BC network	411:433	an ultrafine BC network	411:433	HA nanocrystals embedded in an ultrafine BC network were confirmed and characterized trough different instrumental techniques (SEM, FTIR, XRD, EDS, surface charge and BET analysis), describing its nanostructure, chemical composition and thermal stability.
33069824	0	31	theme	cellulose	42:50	arg1	nanocomposite					15:27	An ecofriendly nanocomposite	0:27	An ecofriendly nanocomposite of bacterial cellulose and hydroxyapatite	0:69	An ecofriendly nanocomposite of bacterial cellulose and hydroxyapatite efficiently removes lead from water.
33069824	0	31	theme	cellulose	42:50	arg1	cellulose					42:50	bacterial cellulose	32:50	bacterial cellulose	32:50	An ecofriendly nanocomposite of bacterial cellulose and hydroxyapatite efficiently removes lead from water.
33069824	0	31	theme	cellulose	42:50	arg1	hydroxyapatite					56:69	hydroxyapatite	56:69	hydroxyapatite	56:69	An ecofriendly nanocomposite of bacterial cellulose and hydroxyapatite efficiently removes lead from water.
33069824	2	32	theme	surface	531:537	arg1	charge					539:544	surface charge	531:544	surface charge	531:544	HA nanocrystals embedded in an ultrafine BC network were confirmed and characterized trough different instrumental techniques (SEM, FTIR, XRD, EDS, surface charge and BET analysis), describing its nanostructure, chemical composition and thermal stability.
33069824	2	32	theme	surface	531:537	arg1	SEM					510:512	SEM	510:512	SEM	510:512	HA nanocrystals embedded in an ultrafine BC network were confirmed and characterized trough different instrumental techniques (SEM, FTIR, XRD, EDS, surface charge and BET analysis), describing its nanostructure, chemical composition and thermal stability.
33069824	3	33	theme	adsorptive	643:652	arg1	removal					654:660	The adsorptive removal	639:660	The adsorptive removal of lead ions by the nanocomposite	639:694	The adsorptive removal of lead ions by the nanocomposite was investigated through batch experiments conducted under different pH, contact times and Pb(II) initial concentrations, proving that the process was highly favorable according to the Langmuir isotherm model (monolayer adsorption) with chemisorption as the main mechanism and kinetic data obeying a nonlinear pseudo-second order kinetic model.
33069824	3	34	theme	monolayer	906:914	arg1	adsorption					916:925	the Langmuir isotherm model (monolayer adsorption)	877:926	the Langmuir isotherm model (monolayer adsorption)	877:926	The adsorptive removal of lead ions by the nanocomposite was investigated through batch experiments conducted under different pH, contact times and Pb(II) initial concentrations, proving that the process was highly favorable according to the Langmuir isotherm model (monolayer adsorption) with chemisorption as the main mechanism and kinetic data obeying a nonlinear pseudo-second order kinetic model.
33069824	2	35	theme	ultrafine	414:422	arg1	network					427:433	an ultrafine BC network	411:433	an ultrafine BC network	411:433	HA nanocrystals embedded in an ultrafine BC network were confirmed and characterized trough different instrumental techniques (SEM, FTIR, XRD, EDS, surface charge and BET analysis), describing its nanostructure, chemical composition and thermal stability.
33069824	0	36	theme	bacterial	32:40	arg1	cellulose					42:50	bacterial cellulose	32:50	bacterial cellulose	32:50	An ecofriendly nanocomposite of bacterial cellulose and hydroxyapatite efficiently removes lead from water.
33069824	1	37	theme	wet	305:307	arg1	technique					332:340	an in situ wet chemical precipitation technique	294:340	an in situ wet chemical precipitation technique	294:340	An environmentally friendly nanocomposite adsorbent composed of two renewable biomaterials, bacterial cellulose (BC) nanofibrils and hydroxyapatite (HA) nanocrystals, was synthetized by an in situ wet chemical precipitation technique, using clam shell biowaste as feedstock.
33069824	2	38	theme	instrumental	485:496	arg1	techniques					498:507	trough different instrumental techniques	468:507	trough different instrumental techniques (SEM, FTIR, XRD, EDS, surface charge and BET analysis)	468:562	HA nanocrystals embedded in an ultrafine BC network were confirmed and characterized trough different instrumental techniques (SEM, FTIR, XRD, EDS, surface charge and BET analysis), describing its nanostructure, chemical composition and thermal stability.
33069824	0	39	theme	hydroxyapatite	56:69	arg1	nanocomposite					15:27	An ecofriendly nanocomposite	0:27	An ecofriendly nanocomposite of bacterial cellulose and hydroxyapatite	0:69	An ecofriendly nanocomposite of bacterial cellulose and hydroxyapatite efficiently removes lead from water.
33069824	0	39	theme	hydroxyapatite	56:69	arg1	cellulose					42:50	bacterial cellulose	32:50	bacterial cellulose	32:50	An ecofriendly nanocomposite of bacterial cellulose and hydroxyapatite efficiently removes lead from water.
33069824	0	39	theme	hydroxyapatite	56:69	arg1	hydroxyapatite					56:69	hydroxyapatite	56:69	hydroxyapatite	56:69	An ecofriendly nanocomposite of bacterial cellulose and hydroxyapatite efficiently removes lead from water.
33069824	1	40	theme	chemical	309:316	arg1	technique					332:340	an in situ wet chemical precipitation technique	294:340	an in situ wet chemical precipitation technique	294:340	An environmentally friendly nanocomposite adsorbent composed of two renewable biomaterials, bacterial cellulose (BC) nanofibrils and hydroxyapatite (HA) nanocrystals, was synthetized by an in situ wet chemical precipitation technique, using clam shell biowaste as feedstock.
33069824	4	41	theme	lead	1269:1272	arg1	removal					1274:1280	lead removal	1269:1280	lead removal	1269:1280	The developed nanocomposite showed a strong removal capacity of Pb(II) both in batch experiments (192 mg/g) and packed-bed column systems (188 mg/g), placing this new nanocomposite among top-performing BC-based biomaterials for lead removal.
33069824	4	42	dep	Pb	1105:1106	arg1	both					1112:1115	both	1112:1115	both	1112:1115	The developed nanocomposite showed a strong removal capacity of Pb(II) both in batch experiments (192 mg/g) and packed-bed column systems (188 mg/g), placing this new nanocomposite among top-performing BC-based biomaterials for lead removal.
33069824	1	43	theme	friendly	127:134	arg1	nanocomposite					136:148	An environmentally friendly nanocomposite adsorbent	108:158	An environmentally friendly nanocomposite adsorbent composed of two renewable biomaterials, bacterial cellulose (BC) nanofibrils and hydroxyapatite (HA) nanocrystals,	108:273	An environmentally friendly nanocomposite adsorbent composed of two renewable biomaterials, bacterial cellulose (BC) nanofibrils and hydroxyapatite (HA) nanocrystals, was synthetized by an in situ wet chemical precipitation technique, using clam shell biowaste as feedstock.
33069824	2	44	theme	trough	468:473	arg1	techniques					498:507	trough different instrumental techniques	468:507	trough different instrumental techniques (SEM, FTIR, XRD, EDS, surface charge and BET analysis)	468:562	HA nanocrystals embedded in an ultrafine BC network were confirmed and characterized trough different instrumental techniques (SEM, FTIR, XRD, EDS, surface charge and BET analysis), describing its nanostructure, chemical composition and thermal stability.
33069824	1	45	theme	precipitation	318:330	arg1	technique					332:340	an in situ wet chemical precipitation technique	294:340	an in situ wet chemical precipitation technique	294:340	An environmentally friendly nanocomposite adsorbent composed of two renewable biomaterials, bacterial cellulose (BC) nanofibrils and hydroxyapatite (HA) nanocrystals, was synthetized by an in situ wet chemical precipitation technique, using clam shell biowaste as feedstock.
33069824	3	46	theme	lead	665:668	arg1	ions					670:673	lead ions	665:673	lead ions	665:673	The adsorptive removal of lead ions by the nanocomposite was investigated through batch experiments conducted under different pH, contact times and Pb(II) initial concentrations, proving that the process was highly favorable according to the Langmuir isotherm model (monolayer adsorption) with chemisorption as the main mechanism and kinetic data obeying a nonlinear pseudo-second order kinetic model.
33069824	4	47	theme	column	1164:1169	arg1	188 mg/g					1180:1187	188 mg/g	1180:1187	188 mg/g	1180:1187	The developed nanocomposite showed a strong removal capacity of Pb(II) both in batch experiments (192 mg/g) and packed-bed column systems (188 mg/g), placing this new nanocomposite among top-performing BC-based biomaterials for lead removal.
33069824	4	47	theme	column	1164:1169	arg1	systems					1171:1177	packed-bed column systems	1153:1177	packed-bed column systems (188 mg/g)	1153:1188	The developed nanocomposite showed a strong removal capacity of Pb(II) both in batch experiments (192 mg/g) and packed-bed column systems (188 mg/g), placing this new nanocomposite among top-performing BC-based biomaterials for lead removal.
33069824	4	48	theme	strong	1078:1083	arg1	capacity					1093:1100	a strong removal capacity	1076:1100	a strong removal capacity of Pb(II) both in batch experiments (192 mg/g) and packed-bed column systems (188 mg/g)	1076:1188	The developed nanocomposite showed a strong removal capacity of Pb(II) both in batch experiments (192 mg/g) and packed-bed column systems (188 mg/g), placing this new nanocomposite among top-performing BC-based biomaterials for lead removal.
33069824	3	49	theme	contact	769:775	arg1	times					777:781	contact times	769:781	contact times	769:781	The adsorptive removal of lead ions by the nanocomposite was investigated through batch experiments conducted under different pH, contact times and Pb(II) initial concentrations, proving that the process was highly favorable according to the Langmuir isotherm model (monolayer adsorption) with chemisorption as the main mechanism and kinetic data obeying a nonlinear pseudo-second order kinetic model.
33069824	3	50	theme	ions	670:673	arg1	removal					654:660	The adsorptive removal	639:660	The adsorptive removal of lead ions by the nanocomposite	639:694	The adsorptive removal of lead ions by the nanocomposite was investigated through batch experiments conducted under different pH, contact times and Pb(II) initial concentrations, proving that the process was highly favorable according to the Langmuir isotherm model (monolayer adsorption) with chemisorption as the main mechanism and kinetic data obeying a nonlinear pseudo-second order kinetic model.
33069824	2	51	from	embedded	399:406	arg1	network					427:433	an ultrafine BC network	411:433	an ultrafine BC network	411:433	HA nanocrystals embedded in an ultrafine BC network were confirmed and characterized trough different instrumental techniques (SEM, FTIR, XRD, EDS, surface charge and BET analysis), describing its nanostructure, chemical composition and thermal stability.
33069824	1	52	theme	adsorbent	150:158	arg1	nanocomposite					136:148	An environmentally friendly nanocomposite adsorbent	108:158	An environmentally friendly nanocomposite adsorbent composed of two renewable biomaterials, bacterial cellulose (BC) nanofibrils and hydroxyapatite (HA) nanocrystals,	108:273	An environmentally friendly nanocomposite adsorbent composed of two renewable biomaterials, bacterial cellulose (BC) nanofibrils and hydroxyapatite (HA) nanocrystals, was synthetized by an in situ wet chemical precipitation technique, using clam shell biowaste as feedstock.
33069824	3	53	theme	main	954:957	arg1	mechanism					959:967	main mechanism	954:967	main mechanism	954:967	The adsorptive removal of lead ions by the nanocomposite was investigated through batch experiments conducted under different pH, contact times and Pb(II) initial concentrations, proving that the process was highly favorable according to the Langmuir isotherm model (monolayer adsorption) with chemisorption as the main mechanism and kinetic data obeying a nonlinear pseudo-second order kinetic model.
33069824	1	54	theme	hydroxyapatite	241:254	arg1	nanocrystals					261:272	hydroxyapatite (HA) nanocrystals	241:272	hydroxyapatite (HA) nanocrystals	241:272	An environmentally friendly nanocomposite adsorbent composed of two renewable biomaterials, bacterial cellulose (BC) nanofibrils and hydroxyapatite (HA) nanocrystals, was synthetized by an in situ wet chemical precipitation technique, using clam shell biowaste as feedstock.
33069824	1	54	theme	hydroxyapatite	241:254	arg1	biomaterials					186:197	two renewable biomaterials	172:197	two renewable biomaterials	172:197	An environmentally friendly nanocomposite adsorbent composed of two renewable biomaterials, bacterial cellulose (BC) nanofibrils and hydroxyapatite (HA) nanocrystals, was synthetized by an in situ wet chemical precipitation technique, using clam shell biowaste as feedstock.
33069824	2	55	theme	BET	550:552	arg1	analysis					554:561	BET analysis	550:561	BET analysis	550:561	HA nanocrystals embedded in an ultrafine BC network were confirmed and characterized trough different instrumental techniques (SEM, FTIR, XRD, EDS, surface charge and BET analysis), describing its nanostructure, chemical composition and thermal stability.
33069824	2	55	theme	BET	550:552	arg1	SEM					510:512	SEM	510:512	SEM	510:512	HA nanocrystals embedded in an ultrafine BC network were confirmed and characterized trough different instrumental techniques (SEM, FTIR, XRD, EDS, surface charge and BET analysis), describing its nanostructure, chemical composition and thermal stability.
33069824	4	56	theme	BC-based	1243:1250	arg1	biomaterials					1252:1263	top-performing BC-based biomaterials	1228:1263	top-performing BC-based biomaterials for lead removal	1228:1280	The developed nanocomposite showed a strong removal capacity of Pb(II) both in batch experiments (192 mg/g) and packed-bed column systems (188 mg/g), placing this new nanocomposite among top-performing BC-based biomaterials for lead removal.
33069824	4	57	theme	removal	1085:1091	arg1	capacity					1093:1100	a strong removal capacity	1076:1100	a strong removal capacity of Pb(II) both in batch experiments (192 mg/g) and packed-bed column systems (188 mg/g)	1076:1188	The developed nanocomposite showed a strong removal capacity of Pb(II) both in batch experiments (192 mg/g) and packed-bed column systems (188 mg/g), placing this new nanocomposite among top-performing BC-based biomaterials for lead removal.
33069824	4	58	theme	Pb	1105:1106	arg1	capacity					1093:1100	a strong removal capacity	1076:1100	a strong removal capacity of Pb(II) both in batch experiments (192 mg/g) and packed-bed column systems (188 mg/g)	1076:1188	The developed nanocomposite showed a strong removal capacity of Pb(II) both in batch experiments (192 mg/g) and packed-bed column systems (188 mg/g), placing this new nanocomposite among top-performing BC-based biomaterials for lead removal.
33069824	4	59	theme	top-performing	1228:1241	arg1	biomaterials					1252:1263	top-performing BC-based biomaterials	1228:1263	top-performing BC-based biomaterials for lead removal	1228:1280	The developed nanocomposite showed a strong removal capacity of Pb(II) both in batch experiments (192 mg/g) and packed-bed column systems (188 mg/g), placing this new nanocomposite among top-performing BC-based biomaterials for lead removal.
33069824	3	60	theme	kinetic	973:979	arg1	data					981:984	kinetic data	973:984	kinetic data	973:984	The adsorptive removal of lead ions by the nanocomposite was investigated through batch experiments conducted under different pH, contact times and Pb(II) initial concentrations, proving that the process was highly favorable according to the Langmuir isotherm model (monolayer adsorption) with chemisorption as the main mechanism and kinetic data obeying a nonlinear pseudo-second order kinetic model.
33069824	2	61	theme	embedded	399:406	arg1	nanocrystals					386:397	HA nanocrystals	383:397	HA nanocrystals embedded in an ultrafine BC network	383:433	HA nanocrystals embedded in an ultrafine BC network were confirmed and characterized trough different instrumental techniques (SEM, FTIR, XRD, EDS, surface charge and BET analysis), describing its nanostructure, chemical composition and thermal stability.
33069824	2	62	theme	different	475:483	arg1	techniques					498:507	trough different instrumental techniques	468:507	trough different instrumental techniques (SEM, FTIR, XRD, EDS, surface charge and BET analysis)	468:562	HA nanocrystals embedded in an ultrafine BC network were confirmed and characterized trough different instrumental techniques (SEM, FTIR, XRD, EDS, surface charge and BET analysis), describing its nanostructure, chemical composition and thermal stability.
33069824	1	63	dep	in	297:298	arg1	situ					300:303	situ	300:303	situ	300:303	An environmentally friendly nanocomposite adsorbent composed of two renewable biomaterials, bacterial cellulose (BC) nanofibrils and hydroxyapatite (HA) nanocrystals, was synthetized by an in situ wet chemical precipitation technique, using clam shell biowaste as feedstock.
33069824	2	64	from	network	427:433	arg1	embedded					399:406	embedded	399:406	embedded	399:406	HA nanocrystals embedded in an ultrafine BC network were confirmed and characterized trough different instrumental techniques (SEM, FTIR, XRD, EDS, surface charge and BET analysis), describing its nanostructure, chemical composition and thermal stability.
33436248	0	0	theme	smart	105:109	arg1	materials					126:134	biodegradable smart food packaging materials	91:134	biodegradable smart food packaging materials	91:134	Carbohydrate-based films containing pH-sensitive red barberry anthocyanins: Application as biodegradable smart food packaging materials.
33436248	0	1	theme	biodegradable	91:103	arg1	materials					126:134	biodegradable smart food packaging materials	91:134	biodegradable smart food packaging materials	91:134	Carbohydrate-based films containing pH-sensitive red barberry anthocyanins: Application as biodegradable smart food packaging materials.
33436248	3	2	theme	food	516:519	arg1	spoilage					521:528	food spoilage	516:528	food spoilage	516:528	Moreover, the RBAs could be used as colorimetric indicators to detect food spoilage because they are sensitive to changes in pH and ammonia gas production.
33436248	2	3	theme	CNFs	340:343	arg1	incorporation					323:335	The incorporation	319:335	The incorporation of CNFs and RBAs	319:352	The incorporation of CNFs and RBAs improved their mechanical properties, moisture resistance, and UV-vis screening properties.
33436248	0	4	theme	packaging	116:124	arg1	materials					126:134	biodegradable smart food packaging materials	91:134	biodegradable smart food packaging materials	91:134	Carbohydrate-based films containing pH-sensitive red barberry anthocyanins: Application as biodegradable smart food packaging materials.
33436248	7	5	theme	grade	1083:1087	arg1	ingredients					1089:1099	food grade ingredients	1078:1099	food grade ingredients that can protect and monitor the freshness of products	1078:1154	Overall, this study shows that active/smart films can be assembled from food grade ingredients that can protect and monitor the freshness of products, like meat and fish.
33436248	4	6	theme	vapor	744:748	arg1	concentration					750:762	ammonia vapor concentration	736:762	ammonia vapor concentration	736:762	The RBA-halochromic indicator changed from reddish/crimson → pink → yellow with increasing pH, and from pink → yellow with increasing ammonia vapor concentration.
33436248	0	7	theme	food	111:114	arg1	materials					126:134	biodegradable smart food packaging materials	91:134	biodegradable smart food packaging materials	91:134	Carbohydrate-based films containing pH-sensitive red barberry anthocyanins: Application as biodegradable smart food packaging materials.
33436248	3	8	theme	colorimetric	482:493	arg1	indicators					495:504	colorimetric indicators	482:504	colorimetric indicators	482:504	Moreover, the RBAs could be used as colorimetric indicators to detect food spoilage because they are sensitive to changes in pH and ammonia gas production.
33436248	3	8	theme	colorimetric	482:493	arg1	RBAs					460:463	the RBAs	456:463	the RBAs	456:463	Moreover, the RBAs could be used as colorimetric indicators to detect food spoilage because they are sensitive to changes in pH and ammonia gas production.
33436248	4	9	theme	pink	663:666	arg1	yellow					670:675	reddish/crimson → pink → yellow	645:675	reddish/crimson → pink → yellow	645:675	The RBA-halochromic indicator changed from reddish/crimson → pink → yellow with increasing pH, and from pink → yellow with increasing ammonia vapor concentration.
33436248	2	10	theme	moisture	392:399	arg1	resistance					401:410	moisture resistance	392:410	moisture resistance	392:410	The incorporation of CNFs and RBAs improved their mechanical properties, moisture resistance, and UV-vis screening properties.
33436248	6	11	theme	food	976:979	arg1	freshness/spoilage					946:963	the freshness/spoilage	942:963	the freshness/spoilage of a model food (fish)	942:986	Finally, the validity of the indicator to monitor the freshness/spoilage of a model food (fish) was demonstrated.
33436248	5	12	theme	smart	782:786	arg1	films					788:792	the smart films	778:792	the smart films	778:792	Furthermore, the smart films possessed good antioxidant and antimicrobial activity owing to the presence of the RBAs and CNFs.
33436248	0	13	contain	containing	25:34	arg2	anthocyanins					62:73	pH-sensitive red barberry anthocyanins	36:73	pH-sensitive red barberry anthocyanins	36:73	Carbohydrate-based films containing pH-sensitive red barberry anthocyanins: Application as biodegradable smart food packaging materials.
33436248	0	13	contain	containing	25:34	arg1	films					19:23	Carbohydrate-based films	0:23	Carbohydrate-based films containing pH-sensitive red barberry anthocyanins	0:73	Carbohydrate-based films containing pH-sensitive red barberry anthocyanins: Application as biodegradable smart food packaging materials.
33436248	4	14	theme	→	668:668	arg1	yellow					670:675	reddish/crimson → pink → yellow	645:675	reddish/crimson → pink → yellow	645:675	The RBA-halochromic indicator changed from reddish/crimson → pink → yellow with increasing pH, and from pink → yellow with increasing ammonia vapor concentration.
33436248	5	15	theme	antioxidant	809:819	arg1	activity					839:846	good antioxidant and antimicrobial activity	804:846	good antioxidant and antimicrobial activity owing to the presence of the RBAs and CNFs	804:889	Furthermore, the smart films possessed good antioxidant and antimicrobial activity owing to the presence of the RBAs and CNFs.
33436248	4	16	theme	ammonia	736:742	arg1	concentration					750:762	ammonia vapor concentration	736:762	ammonia vapor concentration	736:762	The RBA-halochromic indicator changed from reddish/crimson → pink → yellow with increasing pH, and from pink → yellow with increasing ammonia vapor concentration.
33436248	7	17	theme	products	1147:1154	arg1	freshness					1134:1142	the freshness	1130:1142	the freshness of products	1130:1154	Overall, this study shows that active/smart films can be assembled from food grade ingredients that can protect and monitor the freshness of products, like meat and fish.
33436248	1	18	theme	red	211:213	arg1	RBAs					238:241	RBAs	238:241	RBAs	238:241	A novel pH-sensitive colorimetric film was prepared based on immobilizing red barberry anthocyanins (RBAs) within composite chitin nanofiber (CNF) and methylcellulose (MC) matrices.
33436248	1	18	theme	red	211:213	arg1	anthocyanins					224:235	red barberry anthocyanins	211:235	red barberry anthocyanins (RBAs)	211:242	A novel pH-sensitive colorimetric film was prepared based on immobilizing red barberry anthocyanins (RBAs) within composite chitin nanofiber (CNF) and methylcellulose (MC) matrices.
33436248	0	19	theme	Carbohydrate-based	0:17	arg1	films					19:23	Carbohydrate-based films	0:23	Carbohydrate-based films containing pH-sensitive red barberry anthocyanins	0:73	Carbohydrate-based films containing pH-sensitive red barberry anthocyanins: Application as biodegradable smart food packaging materials.
33436248	6	20	theme	model	970:974	arg1	fish					982:985	fish	982:985	fish	982:985	Finally, the validity of the indicator to monitor the freshness/spoilage of a model food (fish) was demonstrated.
33436248	6	20	theme	model	970:974	arg1	food					976:979	a model food	968:979	a model food (fish)	968:986	Finally, the validity of the indicator to monitor the freshness/spoilage of a model food (fish) was demonstrated.
33436248	7	21	theme	active/smart	1037:1048	arg1	films					1050:1054	active/smart films	1037:1054	active/smart films	1037:1054	Overall, this study shows that active/smart films can be assembled from food grade ingredients that can protect and monitor the freshness of products, like meat and fish.
33436248	1	22	theme	barberry	215:222	arg1	RBAs					238:241	RBAs	238:241	RBAs	238:241	A novel pH-sensitive colorimetric film was prepared based on immobilizing red barberry anthocyanins (RBAs) within composite chitin nanofiber (CNF) and methylcellulose (MC) matrices.
33436248	1	22	theme	barberry	215:222	arg1	anthocyanins					224:235	red barberry anthocyanins	211:235	red barberry anthocyanins (RBAs)	211:242	A novel pH-sensitive colorimetric film was prepared based on immobilizing red barberry anthocyanins (RBAs) within composite chitin nanofiber (CNF) and methylcellulose (MC) matrices.
33436248	0	23	theme	pH-sensitive	36:47	arg1	anthocyanins					62:73	pH-sensitive red barberry anthocyanins	36:73	pH-sensitive red barberry anthocyanins	36:73	Carbohydrate-based films containing pH-sensitive red barberry anthocyanins: Application as biodegradable smart food packaging materials.
33436248	3	24	used	used	474:477	arg2	RBAs					460:463	the RBAs	456:463	the RBAs	456:463	Moreover, the RBAs could be used as colorimetric indicators to detect food spoilage because they are sensitive to changes in pH and ammonia gas production.
33436248	3	24	used	used	474:477	arg2	indicators					495:504	colorimetric indicators	482:504	colorimetric indicators	482:504	Moreover, the RBAs could be used as colorimetric indicators to detect food spoilage because they are sensitive to changes in pH and ammonia gas production.
33436248	7	25	theme	food	1078:1081	arg1	ingredients					1089:1099	food grade ingredients	1078:1099	food grade ingredients that can protect and monitor the freshness of products	1078:1154	Overall, this study shows that active/smart films can be assembled from food grade ingredients that can protect and monitor the freshness of products, like meat and fish.
33436248	4	26	theme	reddish/crimson	645:659	arg1	yellow					670:675	reddish/crimson → pink → yellow	645:675	reddish/crimson → pink → yellow	645:675	The RBA-halochromic indicator changed from reddish/crimson → pink → yellow with increasing pH, and from pink → yellow with increasing ammonia vapor concentration.
33436248	0	27	dep	Application	76:86	arg1	films					19:23	Carbohydrate-based films	0:23	Carbohydrate-based films containing pH-sensitive red barberry anthocyanins	0:73	Carbohydrate-based films containing pH-sensitive red barberry anthocyanins: Application as biodegradable smart food packaging materials.
33436248	2	28	theme	screening	424:432	arg1	properties					434:443	UV-vis screening properties	417:443	UV-vis screening properties	417:443	The incorporation of CNFs and RBAs improved their mechanical properties, moisture resistance, and UV-vis screening properties.
33436248	0	29	theme	barberry	53:60	arg1	anthocyanins					62:73	pH-sensitive red barberry anthocyanins	36:73	pH-sensitive red barberry anthocyanins	36:73	Carbohydrate-based films containing pH-sensitive red barberry anthocyanins: Application as biodegradable smart food packaging materials.
33436248	4	30	theme	pink	706:709	arg1	→					711:711	pink →	706:711	pink →	706:711	The RBA-halochromic indicator changed from reddish/crimson → pink → yellow with increasing pH, and from pink → yellow with increasing ammonia vapor concentration.
33436248	1	31	dep	nanofiber	268:276	arg1	matrices					309:316	matrices	309:316	matrices	309:316	A novel pH-sensitive colorimetric film was prepared based on immobilizing red barberry anthocyanins (RBAs) within composite chitin nanofiber (CNF) and methylcellulose (MC) matrices.
33436248	5	32	dep	RBAs	877:880	arg1	the					873:875	the	873:875	the	873:875	Furthermore, the smart films possessed good antioxidant and antimicrobial activity owing to the presence of the RBAs and CNFs.
33436248	2	33	theme	UV-vis	417:422	arg1	properties					434:443	UV-vis screening properties	417:443	UV-vis screening properties	417:443	The incorporation of CNFs and RBAs improved their mechanical properties, moisture resistance, and UV-vis screening properties.
33436248	0	34	theme	red	49:51	arg1	anthocyanins					62:73	pH-sensitive red barberry anthocyanins	36:73	pH-sensitive red barberry anthocyanins	36:73	Carbohydrate-based films containing pH-sensitive red barberry anthocyanins: Application as biodegradable smart food packaging materials.
33436248	5	35	theme	RBAs	877:880	arg1	presence					861:868	the presence	857:868	the presence of the RBAs and CNFs	857:889	Furthermore, the smart films possessed good antioxidant and antimicrobial activity owing to the presence of the RBAs and CNFs.
33436248	5	36	theme	antimicrobial	825:837	arg1	activity					839:846	good antioxidant and antimicrobial activity	804:846	good antioxidant and antimicrobial activity owing to the presence of the RBAs and CNFs	804:889	Furthermore, the smart films possessed good antioxidant and antimicrobial activity owing to the presence of the RBAs and CNFs.
33436248	4	37	theme	→	661:661	arg1	yellow					670:675	reddish/crimson → pink → yellow	645:675	reddish/crimson → pink → yellow	645:675	The RBA-halochromic indicator changed from reddish/crimson → pink → yellow with increasing pH, and from pink → yellow with increasing ammonia vapor concentration.
33436248	3	38	theme	ammonia	578:584	arg1	production					590:599	ammonia gas production	578:599	ammonia gas production	578:599	Moreover, the RBAs could be used as colorimetric indicators to detect food spoilage because they are sensitive to changes in pH and ammonia gas production.
33436248	2	39	theme	RBAs	349:352	arg1	incorporation					323:335	The incorporation	319:335	The incorporation of CNFs and RBAs	319:352	The incorporation of CNFs and RBAs improved their mechanical properties, moisture resistance, and UV-vis screening properties.
33436248	5	40	theme	good	804:807	arg1	activity					839:846	good antioxidant and antimicrobial activity	804:846	good antioxidant and antimicrobial activity owing to the presence of the RBAs and CNFs	804:889	Furthermore, the smart films possessed good antioxidant and antimicrobial activity owing to the presence of the RBAs and CNFs.
33436248	3	41	theme	gas	586:588	arg1	production					590:599	ammonia gas production	578:599	ammonia gas production	578:599	Moreover, the RBAs could be used as colorimetric indicators to detect food spoilage because they are sensitive to changes in pH and ammonia gas production.
33436248	1	42	theme	novel	139:143	arg1	film					171:174	A novel pH-sensitive colorimetric film	137:174	A novel pH-sensitive colorimetric film	137:174	A novel pH-sensitive colorimetric film was prepared based on immobilizing red barberry anthocyanins (RBAs) within composite chitin nanofiber (CNF) and methylcellulose (MC) matrices.
33436248	3	43	from	changes	560:566	arg1	production					590:599	ammonia gas production	578:599	ammonia gas production	578:599	Moreover, the RBAs could be used as colorimetric indicators to detect food spoilage because they are sensitive to changes in pH and ammonia gas production.
33436248	3	43	from	changes	560:566	arg1	pH					571:572	pH	571:572	pH	571:572	Moreover, the RBAs could be used as colorimetric indicators to detect food spoilage because they are sensitive to changes in pH and ammonia gas production.
33436248	6	44	theme	indicator	921:929	arg1	validity					905:912	the validity	901:912	the validity of the indicator to monitor the freshness/spoilage of a model food (fish)	901:986	Finally, the validity of the indicator to monitor the freshness/spoilage of a model food (fish) was demonstrated.
33436248	5	45	contain	possessed	794:802	arg2	activity					839:846	good antioxidant and antimicrobial activity	804:846	good antioxidant and antimicrobial activity owing to the presence of the RBAs and CNFs	804:889	Furthermore, the smart films possessed good antioxidant and antimicrobial activity owing to the presence of the RBAs and CNFs.
33436248	5	45	contain	possessed	794:802	arg1	films					788:792	the smart films	778:792	the smart films	778:792	Furthermore, the smart films possessed good antioxidant and antimicrobial activity owing to the presence of the RBAs and CNFs.
33436248	4	46	dep	increasing	682:691	arg1	yellow					713:718	yellow	713:718	yellow with increasing ammonia vapor concentration	713:762	The RBA-halochromic indicator changed from reddish/crimson → pink → yellow with increasing pH, and from pink → yellow with increasing ammonia vapor concentration.
33436248	1	47	theme	pH-sensitive	145:156	arg1	film					171:174	A novel pH-sensitive colorimetric film	137:174	A novel pH-sensitive colorimetric film	137:174	A novel pH-sensitive colorimetric film was prepared based on immobilizing red barberry anthocyanins (RBAs) within composite chitin nanofiber (CNF) and methylcellulose (MC) matrices.
33436248	4	48	theme	RBA-halochromic	606:620	arg1	indicator					622:630	The RBA-halochromic indicator	602:630	The RBA-halochromic indicator	602:630	The RBA-halochromic indicator changed from reddish/crimson → pink → yellow with increasing pH, and from pink → yellow with increasing ammonia vapor concentration.
33436248	1	49	theme	colorimetric	158:169	arg1	film					171:174	A novel pH-sensitive colorimetric film	137:174	A novel pH-sensitive colorimetric film	137:174	A novel pH-sensitive colorimetric film was prepared based on immobilizing red barberry anthocyanins (RBAs) within composite chitin nanofiber (CNF) and methylcellulose (MC) matrices.
33436248	1	50	theme	composite	251:259	arg1	CNF					279:281	CNF	279:281	CNF	279:281	A novel pH-sensitive colorimetric film was prepared based on immobilizing red barberry anthocyanins (RBAs) within composite chitin nanofiber (CNF) and methylcellulose (MC) matrices.
33436248	1	50	theme	composite	251:259	arg1	nanofiber					268:276	composite chitin nanofiber	251:276	composite chitin nanofiber (CNF)	251:282	A novel pH-sensitive colorimetric film was prepared based on immobilizing red barberry anthocyanins (RBAs) within composite chitin nanofiber (CNF) and methylcellulose (MC) matrices.
33436248	5	51	theme	CNFs	886:889	arg1	presence					861:868	the presence	857:868	the presence of the RBAs and CNFs	857:889	Furthermore, the smart films possessed good antioxidant and antimicrobial activity owing to the presence of the RBAs and CNFs.
33436248	1	52	theme	chitin	261:266	arg1	CNF					279:281	CNF	279:281	CNF	279:281	A novel pH-sensitive colorimetric film was prepared based on immobilizing red barberry anthocyanins (RBAs) within composite chitin nanofiber (CNF) and methylcellulose (MC) matrices.
33436248	1	52	theme	chitin	261:266	arg1	nanofiber					268:276	composite chitin nanofiber	251:276	composite chitin nanofiber (CNF)	251:282	A novel pH-sensitive colorimetric film was prepared based on immobilizing red barberry anthocyanins (RBAs) within composite chitin nanofiber (CNF) and methylcellulose (MC) matrices.
33436248	2	53	theme	mechanical	369:378	arg1	properties					380:389	their mechanical properties	363:389	their mechanical properties	363:389	The incorporation of CNFs and RBAs improved their mechanical properties, moisture resistance, and UV-vis screening properties.
34481906	0	0	theme	stable	74:79	arg1	formulation					92:102	a safe and stable injectable formulation	63:102	a safe and stable injectable formulation	63:102	Design of ultra-fine carvedilol nanococrystals: Development of a safe and stable injectable formulation.
34481906	4	1	theme	concentrations	632:645	arg1	impact					586:591	the impact	582:591	the impact of CAR, TA, poloxamer 188 (stabilizer) concentrations, and anti-solvent/solvent ratio on CAR NCCs particle size (PS)	582:708	Central Composite Design (CCD) considering the impact of CAR, TA, poloxamer 188 (stabilizer) concentrations, and anti-solvent/solvent ratio on CAR NCCs particle size (PS) could produce ultra-fine NCCs (about 1 nm).
34481906	1	2	theme	beta-blocker	137:148	arg1	Carvedilol					105:114	Carvedilol	105:114	Carvedilol (CAR)	105:120	Carvedilol (CAR) is a strategic beta-blocker agent which its application has been limited by its very low water solubility.
34481906	1	2	theme	beta-blocker	137:148	arg1	agent					150:154	a strategic beta-blocker agent	125:154	a strategic beta-blocker agent which its application has been limited by its very low water solubility	125:226	Carvedilol (CAR) is a strategic beta-blocker agent which its application has been limited by its very low water solubility.
34481906	0	3	theme	ultra-fine	10:19	arg1	nanococrystals					32:45	ultra-fine carvedilol nanococrystals	10:45	ultra-fine carvedilol nanococrystals	10:45	Design of ultra-fine carvedilol nanococrystals: Development of a safe and stable injectable formulation.
34481906	4	4	theme	Composite	547:555	arg1	Design					557:562	Central Composite Design	539:562	Central Composite Design (CCD) considering the impact of CAR, TA, poloxamer 188 (stabilizer) concentrations, and anti-solvent/solvent ratio on CAR NCCs particle size (PS)	539:708	Central Composite Design (CCD) considering the impact of CAR, TA, poloxamer 188 (stabilizer) concentrations, and anti-solvent/solvent ratio on CAR NCCs particle size (PS) could produce ultra-fine NCCs (about 1 nm).
34481906	4	4	theme	Composite	547:555	arg1	CCD					565:567	CCD	565:567	CCD	565:567	Central Composite Design (CCD) considering the impact of CAR, TA, poloxamer 188 (stabilizer) concentrations, and anti-solvent/solvent ratio on CAR NCCs particle size (PS) could produce ultra-fine NCCs (about 1 nm).
34481906	5	5	theme	various	821:827	arg1	types					829:833	various types	821:833	various types	821:833	The lyophilization of NCCs investigating slow/fast freezing rates, various types and concentrations of cryprotectants and lyoprotectants indicated that PEG and trehalose (5 % w/vconcentration) under slow freezing rate could re-produce the initial PSs successfully.
34481906	2	6	theme	anti-solvent	310:321	arg1	technique					337:345	nano-cocrystal (NCC) anti-solvent precipitation technique	289:345	nano-cocrystal (NCC) anti-solvent precipitation technique	289:345	The present study describes a soluble form of drug based on nano-cocrystal (NCC) anti-solvent precipitation technique.
34481906	4	7	theme	Central	539:545	arg1	Design					557:562	Central Composite Design	539:562	Central Composite Design (CCD) considering the impact of CAR, TA, poloxamer 188 (stabilizer) concentrations, and anti-solvent/solvent ratio on CAR NCCs particle size (PS)	539:708	Central Composite Design (CCD) considering the impact of CAR, TA, poloxamer 188 (stabilizer) concentrations, and anti-solvent/solvent ratio on CAR NCCs particle size (PS) could produce ultra-fine NCCs (about 1 nm).
34481906	4	7	theme	Central	539:545	arg1	CCD					565:567	CCD	565:567	CCD	565:567	Central Composite Design (CCD) considering the impact of CAR, TA, poloxamer 188 (stabilizer) concentrations, and anti-solvent/solvent ratio on CAR NCCs particle size (PS) could produce ultra-fine NCCs (about 1 nm).
34481906	0	8	theme	formulation	92:102	arg1	Development					48:58	Development	48:58	Design of ultra-fine carvedilol nanococrystals: Development of a safe and stable injectable formulation.	0:103	Design of ultra-fine carvedilol nanococrystals: Development of a safe and stable injectable formulation.
34481906	5	9	theme	%	927:927	arg1	w/vconcentration					929:944	5 % w/vconcentration	925:944	5 % w/vconcentration	925:944	The lyophilization of NCCs investigating slow/fast freezing rates, various types and concentrations of cryprotectants and lyoprotectants indicated that PEG and trehalose (5 % w/vconcentration) under slow freezing rate could re-produce the initial PSs successfully.
34481906	5	9	theme	%	927:927	arg1	PEG					906:908	PEG	906:908	PEG	906:908	The lyophilization of NCCs investigating slow/fast freezing rates, various types and concentrations of cryprotectants and lyoprotectants indicated that PEG and trehalose (5 % w/vconcentration) under slow freezing rate could re-produce the initial PSs successfully.
34481906	7	10	theme	amorphous	1240:1248	arg1	structures					1250:1259	more amorphous structures	1235:1259	more amorphous structures	1235:1259	DSC and PXRD experiments proved that the formulations containing trehalose led to more crystalline and the ones comprising PEG led to more amorphous structures.
34481906	0	11	theme	injectable	81:90	arg1	formulation					92:102	a safe and stable injectable formulation	63:102	a safe and stable injectable formulation	63:102	Design of ultra-fine carvedilol nanococrystals: Development of a safe and stable injectable formulation.
34481906	9	12	theme	easy	1479:1482	arg1	urgencies					1469:1477	hypertension urgencies	1456:1477	hypertension urgencies easy for industrial scale-up	1456:1506	In conclusion, the NCC technology could produce the first safe soluble form of CAR for treating hypertension urgencies easy for industrial scale-up.
34481906	7	13	theme	more	1183:1186	arg1	crystalline					1188:1198	more crystalline	1183:1198	more crystalline	1183:1198	DSC and PXRD experiments proved that the formulations containing trehalose led to more crystalline and the ones comprising PEG led to more amorphous structures.
34481906	2	14	theme	soluble	259:265	arg1	form					267:270	a soluble form	257:270	a soluble form of drug based on nano-cocrystal (NCC) anti-solvent precipitation technique	257:345	The present study describes a soluble form of drug based on nano-cocrystal (NCC) anti-solvent precipitation technique.
34481906	4	15	theme	anti-solvent/solvent	652:671	arg1	ratio					673:677	anti-solvent/solvent ratio	652:677	anti-solvent/solvent ratio	652:677	Central Composite Design (CCD) considering the impact of CAR, TA, poloxamer 188 (stabilizer) concentrations, and anti-solvent/solvent ratio on CAR NCCs particle size (PS) could produce ultra-fine NCCs (about 1 nm).
34481906	7	16	theme	DSC	1101:1103	arg1	experiments					1114:1124	DSC and PXRD experiments	1101:1124	DSC and PXRD experiments	1101:1124	DSC and PXRD experiments proved that the formulations containing trehalose led to more crystalline and the ones comprising PEG led to more amorphous structures.
34481906	4	17	theme	CAR	682:684	arg1	PS					706:707	PS	706:707	PS	706:707	Central Composite Design (CCD) considering the impact of CAR, TA, poloxamer 188 (stabilizer) concentrations, and anti-solvent/solvent ratio on CAR NCCs particle size (PS) could produce ultra-fine NCCs (about 1 nm).
34481906	4	17	theme	CAR	682:684	arg1	size					700:703	CAR NCCs particle size	682:703	CAR NCCs particle size (PS)	682:708	Central Composite Design (CCD) considering the impact of CAR, TA, poloxamer 188 (stabilizer) concentrations, and anti-solvent/solvent ratio on CAR NCCs particle size (PS) could produce ultra-fine NCCs (about 1 nm).
34481906	8	18	theme	PEG	1294:1296	arg1	NCCs					1308:1311	the slow freezed PEG protected NCCs	1277:1311	the slow freezed PEG protected NCCs	1277:1311	Interestingly, the slow freezed PEG protected NCCs were physically stable for at least 18 months.
34481906	8	18	theme	PEG	1294:1296	arg1	stable					1329:1334	stable	1329:1334	stable	1329:1334	Interestingly, the slow freezed PEG protected NCCs were physically stable for at least 18 months.
34481906	4	19	theme	188	615:617	arg1	concentrations					632:645	poloxamer 188 (stabilizer) concentrations	605:645	poloxamer 188 (stabilizer) concentrations	605:645	Central Composite Design (CCD) considering the impact of CAR, TA, poloxamer 188 (stabilizer) concentrations, and anti-solvent/solvent ratio on CAR NCCs particle size (PS) could produce ultra-fine NCCs (about 1 nm).
34481906	4	20	dep	NCCs	735:738	arg1	1 nm					747:750	1 nm	747:750	1 nm	747:750	Central Composite Design (CCD) considering the impact of CAR, TA, poloxamer 188 (stabilizer) concentrations, and anti-solvent/solvent ratio on CAR NCCs particle size (PS) could produce ultra-fine NCCs (about 1 nm).
34481906	3	21	theme	enthalpy	481:488	arg1	changes					490:496	the enthalpy changes	477:496	the enthalpy changes of cocrystallization and solubilization	477:536	The COSMOquick software was employed to select the optimum coformer (tartaric acid, TA) and organic solvent (acetone) relying on the enthalpy changes of cocrystallization and solubilization.
34481906	3	22	theme	tartaric	417:424	arg1	TA					432:433	TA	432:433	TA	432:433	The COSMOquick software was employed to select the optimum coformer (tartaric acid, TA) and organic solvent (acetone) relying on the enthalpy changes of cocrystallization and solubilization.
34481906	3	22	theme	tartaric	417:424	arg1	acid					426:429	tartaric acid	417:429	tartaric acid	417:429	The COSMOquick software was employed to select the optimum coformer (tartaric acid, TA) and organic solvent (acetone) relying on the enthalpy changes of cocrystallization and solubilization.
34481906	6	23	theme	CAR	1019:1021	arg1	NCCs					1023:1026	CAR NCCs	1019:1026	CAR NCCs	1019:1026	CAR NCCs indicated about 2000 fold increase in solubility compared with pure CAR.
34481906	9	24	theme	industrial	1488:1497	arg1	scale-up					1499:1506	industrial scale-up	1488:1506	industrial scale-up	1488:1506	In conclusion, the NCC technology could produce the first safe soluble form of CAR for treating hypertension urgencies easy for industrial scale-up.
34481906	3	25	theme	solubilization	523:536	arg1	changes					490:496	the enthalpy changes	477:496	the enthalpy changes of cocrystallization and solubilization	477:536	The COSMOquick software was employed to select the optimum coformer (tartaric acid, TA) and organic solvent (acetone) relying on the enthalpy changes of cocrystallization and solubilization.
34481906	3	26	theme	optimum	399:405	arg1	coformer					407:414	the optimum coformer	395:414	the optimum coformer (tartaric acid, TA)	395:434	The COSMOquick software was employed to select the optimum coformer (tartaric acid, TA) and organic solvent (acetone) relying on the enthalpy changes of cocrystallization and solubilization.
34481906	6	27	dep	fold	1049:1052	arg1	2000					1044:1047	2000	1044:1047	2000	1044:1047	CAR NCCs indicated about 2000 fold increase in solubility compared with pure CAR.
34481906	5	28	theme	initial	993:999	arg1	PSs					1001:1003	the initial PSs	989:1003	the initial PSs	989:1003	The lyophilization of NCCs investigating slow/fast freezing rates, various types and concentrations of cryprotectants and lyoprotectants indicated that PEG and trehalose (5 % w/vconcentration) under slow freezing rate could re-produce the initial PSs successfully.
34481906	5	29	theme	NCCs	776:779	arg1	lyophilization					758:771	The lyophilization	754:771	The lyophilization of NCCs investigating slow/fast freezing rates, various types and concentrations of cryprotectants and lyoprotectants	754:889	The lyophilization of NCCs investigating slow/fast freezing rates, various types and concentrations of cryprotectants and lyoprotectants indicated that PEG and trehalose (5 % w/vconcentration) under slow freezing rate could re-produce the initial PSs successfully.
34481906	5	30	theme	lyoprotectants	876:889	arg1	concentrations					839:852	concentrations	839:852	concentrations of cryprotectants and lyoprotectants	839:889	The lyophilization of NCCs investigating slow/fast freezing rates, various types and concentrations of cryprotectants and lyoprotectants indicated that PEG and trehalose (5 % w/vconcentration) under slow freezing rate could re-produce the initial PSs successfully.
34481906	5	30	theme	lyoprotectants	876:889	arg1	rates					814:818	slow/fast freezing rates	795:818	slow/fast freezing rates	795:818	The lyophilization of NCCs investigating slow/fast freezing rates, various types and concentrations of cryprotectants and lyoprotectants indicated that PEG and trehalose (5 % w/vconcentration) under slow freezing rate could re-produce the initial PSs successfully.
34481906	5	30	theme	lyoprotectants	876:889	arg1	types					829:833	various types	821:833	various types	821:833	The lyophilization of NCCs investigating slow/fast freezing rates, various types and concentrations of cryprotectants and lyoprotectants indicated that PEG and trehalose (5 % w/vconcentration) under slow freezing rate could re-produce the initial PSs successfully.
34481906	4	31	theme	poloxamer	605:613	arg1	stabilizer					620:629	stabilizer	620:629	stabilizer	620:629	Central Composite Design (CCD) considering the impact of CAR, TA, poloxamer 188 (stabilizer) concentrations, and anti-solvent/solvent ratio on CAR NCCs particle size (PS) could produce ultra-fine NCCs (about 1 nm).
34481906	4	31	theme	poloxamer	605:613	arg1	188					615:617	poloxamer 188	605:617	poloxamer 188 (stabilizer) concentrations	605:645	Central Composite Design (CCD) considering the impact of CAR, TA, poloxamer 188 (stabilizer) concentrations, and anti-solvent/solvent ratio on CAR NCCs particle size (PS) could produce ultra-fine NCCs (about 1 nm).
34481906	0	32	theme	carvedilol	21:30	arg1	nanococrystals					32:45	ultra-fine carvedilol nanococrystals	10:45	ultra-fine carvedilol nanococrystals	10:45	Design of ultra-fine carvedilol nanococrystals: Development of a safe and stable injectable formulation.
34481906	5	33	theme	cryprotectants	857:870	arg1	concentrations					839:852	concentrations	839:852	concentrations of cryprotectants and lyoprotectants	839:889	The lyophilization of NCCs investigating slow/fast freezing rates, various types and concentrations of cryprotectants and lyoprotectants indicated that PEG and trehalose (5 % w/vconcentration) under slow freezing rate could re-produce the initial PSs successfully.
34481906	5	33	theme	cryprotectants	857:870	arg1	rates					814:818	slow/fast freezing rates	795:818	slow/fast freezing rates	795:818	The lyophilization of NCCs investigating slow/fast freezing rates, various types and concentrations of cryprotectants and lyoprotectants indicated that PEG and trehalose (5 % w/vconcentration) under slow freezing rate could re-produce the initial PSs successfully.
34481906	5	33	theme	cryprotectants	857:870	arg1	types					829:833	various types	821:833	various types	821:833	The lyophilization of NCCs investigating slow/fast freezing rates, various types and concentrations of cryprotectants and lyoprotectants indicated that PEG and trehalose (5 % w/vconcentration) under slow freezing rate could re-produce the initial PSs successfully.
34481906	3	34	theme	cocrystallization	501:517	arg1	changes					490:496	the enthalpy changes	477:496	the enthalpy changes of cocrystallization and solubilization	477:536	The COSMOquick software was employed to select the optimum coformer (tartaric acid, TA) and organic solvent (acetone) relying on the enthalpy changes of cocrystallization and solubilization.
34481906	7	35	theme	more	1235:1238	arg1	structures					1250:1259	more amorphous structures	1235:1259	more amorphous structures	1235:1259	DSC and PXRD experiments proved that the formulations containing trehalose led to more crystalline and the ones comprising PEG led to more amorphous structures.
34481906	3	36	theme	COSMOquick	352:361	arg1	software					363:370	The COSMOquick software	348:370	The COSMOquick software	348:370	The COSMOquick software was employed to select the optimum coformer (tartaric acid, TA) and organic solvent (acetone) relying on the enthalpy changes of cocrystallization and solubilization.
34481906	4	37	theme	NCCs	686:689	arg1	PS					706:707	PS	706:707	PS	706:707	Central Composite Design (CCD) considering the impact of CAR, TA, poloxamer 188 (stabilizer) concentrations, and anti-solvent/solvent ratio on CAR NCCs particle size (PS) could produce ultra-fine NCCs (about 1 nm).
34481906	4	37	theme	NCCs	686:689	arg1	size					700:703	CAR NCCs particle size	682:703	CAR NCCs particle size (PS)	682:708	Central Composite Design (CCD) considering the impact of CAR, TA, poloxamer 188 (stabilizer) concentrations, and anti-solvent/solvent ratio on CAR NCCs particle size (PS) could produce ultra-fine NCCs (about 1 nm).
34481906	9	38	theme	hypertension	1456:1467	arg1	urgencies					1469:1477	hypertension urgencies	1456:1477	hypertension urgencies easy for industrial scale-up	1456:1506	In conclusion, the NCC technology could produce the first safe soluble form of CAR for treating hypertension urgencies easy for industrial scale-up.
34481906	6	39	theme	pure	1091:1094	arg1	CAR					1096:1098	pure CAR	1091:1098	pure CAR	1091:1098	CAR NCCs indicated about 2000 fold increase in solubility compared with pure CAR.
34481906	7	40	theme	PXRD	1109:1112	arg1	experiments					1114:1124	DSC and PXRD experiments	1101:1124	DSC and PXRD experiments	1101:1124	DSC and PXRD experiments proved that the formulations containing trehalose led to more crystalline and the ones comprising PEG led to more amorphous structures.
34481906	4	41	from	impact	586:591	arg1	PS					706:707	PS	706:707	PS	706:707	Central Composite Design (CCD) considering the impact of CAR, TA, poloxamer 188 (stabilizer) concentrations, and anti-solvent/solvent ratio on CAR NCCs particle size (PS) could produce ultra-fine NCCs (about 1 nm).
34481906	4	41	from	impact	586:591	arg1	size					700:703	CAR NCCs particle size	682:703	CAR NCCs particle size (PS)	682:708	Central Composite Design (CCD) considering the impact of CAR, TA, poloxamer 188 (stabilizer) concentrations, and anti-solvent/solvent ratio on CAR NCCs particle size (PS) could produce ultra-fine NCCs (about 1 nm).
34481906	0	42	theme	nanococrystals	32:45	arg1	Design					0:5	Design	0:5	Design of ultra-fine carvedilol nanococrystals: Development of a safe and stable injectable formulation.	0:103	Design of ultra-fine carvedilol nanococrystals: Development of a safe and stable injectable formulation.
34481906	5	43	theme	slow/fast	795:803	arg1	rates					814:818	slow/fast freezing rates	795:818	slow/fast freezing rates	795:818	The lyophilization of NCCs investigating slow/fast freezing rates, various types and concentrations of cryprotectants and lyoprotectants indicated that PEG and trehalose (5 % w/vconcentration) under slow freezing rate could re-produce the initial PSs successfully.
34481906	9	44	theme	first	1412:1416	arg1	form					1431:1434	the first safe soluble form	1408:1434	the first safe soluble form of CAR for treating hypertension urgencies easy for industrial scale-up	1408:1506	In conclusion, the NCC technology could produce the first safe soluble form of CAR for treating hypertension urgencies easy for industrial scale-up.
34481906	3	45	dep	coformer	407:414	arg1	TA					432:433	TA	432:433	TA	432:433	The COSMOquick software was employed to select the optimum coformer (tartaric acid, TA) and organic solvent (acetone) relying on the enthalpy changes of cocrystallization and solubilization.
34481906	3	45	dep	coformer	407:414	arg1	acid					426:429	tartaric acid	417:429	tartaric acid	417:429	The COSMOquick software was employed to select the optimum coformer (tartaric acid, TA) and organic solvent (acetone) relying on the enthalpy changes of cocrystallization and solubilization.
34481906	6	46	from	increase	1054:1061	arg1	solubility					1066:1075	solubility	1066:1075	solubility	1066:1075	CAR NCCs indicated about 2000 fold increase in solubility compared with pure CAR.
34481906	4	47	theme	particle	691:698	arg1	PS					706:707	PS	706:707	PS	706:707	Central Composite Design (CCD) considering the impact of CAR, TA, poloxamer 188 (stabilizer) concentrations, and anti-solvent/solvent ratio on CAR NCCs particle size (PS) could produce ultra-fine NCCs (about 1 nm).
34481906	4	47	theme	particle	691:698	arg1	size					700:703	CAR NCCs particle size	682:703	CAR NCCs particle size (PS)	682:708	Central Composite Design (CCD) considering the impact of CAR, TA, poloxamer 188 (stabilizer) concentrations, and anti-solvent/solvent ratio on CAR NCCs particle size (PS) could produce ultra-fine NCCs (about 1 nm).
34481906	8	48	theme	slow	1281:1284	arg1	NCCs					1308:1311	the slow freezed PEG protected NCCs	1277:1311	the slow freezed PEG protected NCCs	1277:1311	Interestingly, the slow freezed PEG protected NCCs were physically stable for at least 18 months.
34481906	8	48	theme	slow	1281:1284	arg1	stable					1329:1334	stable	1329:1334	stable	1329:1334	Interestingly, the slow freezed PEG protected NCCs were physically stable for at least 18 months.
34481906	5	49	theme	freezing	958:965	arg1	rate					967:970	slow freezing rate	953:970	slow freezing rate	953:970	The lyophilization of NCCs investigating slow/fast freezing rates, various types and concentrations of cryprotectants and lyoprotectants indicated that PEG and trehalose (5 % w/vconcentration) under slow freezing rate could re-produce the initial PSs successfully.
34481906	8	50	theme	protected	1298:1306	arg1	NCCs					1308:1311	the slow freezed PEG protected NCCs	1277:1311	the slow freezed PEG protected NCCs	1277:1311	Interestingly, the slow freezed PEG protected NCCs were physically stable for at least 18 months.
34481906	8	50	theme	protected	1298:1306	arg1	stable					1329:1334	stable	1329:1334	stable	1329:1334	Interestingly, the slow freezed PEG protected NCCs were physically stable for at least 18 months.
34481906	9	51	theme	safe	1418:1421	arg1	form					1431:1434	the first safe soluble form	1408:1434	the first safe soluble form of CAR for treating hypertension urgencies easy for industrial scale-up	1408:1506	In conclusion, the NCC technology could produce the first safe soluble form of CAR for treating hypertension urgencies easy for industrial scale-up.
34481906	9	52	theme	soluble	1423:1429	arg1	form					1431:1434	the first safe soluble form	1408:1434	the first safe soluble form of CAR for treating hypertension urgencies easy for industrial scale-up	1408:1506	In conclusion, the NCC technology could produce the first safe soluble form of CAR for treating hypertension urgencies easy for industrial scale-up.
34481906	4	53	theme	ultra-fine	724:733	arg1	NCCs					735:738	ultra-fine NCCs	724:738	ultra-fine NCCs (about 1 nm)	724:751	Central Composite Design (CCD) considering the impact of CAR, TA, poloxamer 188 (stabilizer) concentrations, and anti-solvent/solvent ratio on CAR NCCs particle size (PS) could produce ultra-fine NCCs (about 1 nm).
34481906	5	54	theme	freezing	805:812	arg1	rates					814:818	slow/fast freezing rates	795:818	slow/fast freezing rates	795:818	The lyophilization of NCCs investigating slow/fast freezing rates, various types and concentrations of cryprotectants and lyoprotectants indicated that PEG and trehalose (5 % w/vconcentration) under slow freezing rate could re-produce the initial PSs successfully.
34481906	6	55	dep	increase	1054:1061	arg1	fold					1049:1052	fold	1049:1052	fold	1049:1052	CAR NCCs indicated about 2000 fold increase in solubility compared with pure CAR.
34481906	5	56	theme	slow	953:956	arg1	rate					967:970	slow freezing rate	953:970	slow freezing rate	953:970	The lyophilization of NCCs investigating slow/fast freezing rates, various types and concentrations of cryprotectants and lyoprotectants indicated that PEG and trehalose (5 % w/vconcentration) under slow freezing rate could re-produce the initial PSs successfully.
34481906	4	57	theme	ratio	673:677	arg1	impact					586:591	the impact	582:591	the impact of CAR, TA, poloxamer 188 (stabilizer) concentrations, and anti-solvent/solvent ratio on CAR NCCs particle size (PS)	582:708	Central Composite Design (CCD) considering the impact of CAR, TA, poloxamer 188 (stabilizer) concentrations, and anti-solvent/solvent ratio on CAR NCCs particle size (PS) could produce ultra-fine NCCs (about 1 nm).
34481906	2	58	theme	drug	275:278	arg1	form					267:270	a soluble form	257:270	a soluble form of drug based on nano-cocrystal (NCC) anti-solvent precipitation technique	257:345	The present study describes a soluble form of drug based on nano-cocrystal (NCC) anti-solvent precipitation technique.
34481906	8	59	theme	freezed	1286:1292	arg1	NCCs					1308:1311	the slow freezed PEG protected NCCs	1277:1311	the slow freezed PEG protected NCCs	1277:1311	Interestingly, the slow freezed PEG protected NCCs were physically stable for at least 18 months.
34481906	8	59	theme	freezed	1286:1292	arg1	stable					1329:1334	stable	1329:1334	stable	1329:1334	Interestingly, the slow freezed PEG protected NCCs were physically stable for at least 18 months.
34481906	2	60	theme	present	233:239	arg1	study					241:245	The present study	229:245	The present study	229:245	The present study describes a soluble form of drug based on nano-cocrystal (NCC) anti-solvent precipitation technique.
34481906	7	61	contain	containing	1155:1164	arg2	trehalose					1166:1174	trehalose	1166:1174	trehalose	1166:1174	DSC and PXRD experiments proved that the formulations containing trehalose led to more crystalline and the ones comprising PEG led to more amorphous structures.
34481906	7	61	contain	containing	1155:1164	arg1	formulations					1142:1153	the formulations	1138:1153	the formulations containing trehalose	1138:1174	DSC and PXRD experiments proved that the formulations containing trehalose led to more crystalline and the ones comprising PEG led to more amorphous structures.
34481906	3	62	theme	organic	440:446	arg1	solvent					448:454	organic solvent	440:454	organic solvent (acetone)	440:464	The COSMOquick software was employed to select the optimum coformer (tartaric acid, TA) and organic solvent (acetone) relying on the enthalpy changes of cocrystallization and solubilization.
34481906	3	62	theme	organic	440:446	arg1	acetone					457:463	acetone	457:463	acetone	457:463	The COSMOquick software was employed to select the optimum coformer (tartaric acid, TA) and organic solvent (acetone) relying on the enthalpy changes of cocrystallization and solubilization.
34481906	1	63	theme	low	207:209	arg1	solubility					217:226	its very low water solubility	198:226	its very low water solubility	198:226	Carvedilol (CAR) is a strategic beta-blocker agent which its application has been limited by its very low water solubility.
34481906	0	64	theme	safe	65:68	arg1	formulation					92:102	a safe and stable injectable formulation	63:102	a safe and stable injectable formulation	63:102	Design of ultra-fine carvedilol nanococrystals: Development of a safe and stable injectable formulation.
34481906	4	65	theme	CAR	596:598	arg1	impact					586:591	the impact	582:591	the impact of CAR, TA, poloxamer 188 (stabilizer) concentrations, and anti-solvent/solvent ratio on CAR NCCs particle size (PS)	582:708	Central Composite Design (CCD) considering the impact of CAR, TA, poloxamer 188 (stabilizer) concentrations, and anti-solvent/solvent ratio on CAR NCCs particle size (PS) could produce ultra-fine NCCs (about 1 nm).
34481906	9	66	theme	CAR	1439:1441	arg1	form					1431:1434	the first safe soluble form	1408:1434	the first safe soluble form of CAR for treating hypertension urgencies easy for industrial scale-up	1408:1506	In conclusion, the NCC technology could produce the first safe soluble form of CAR for treating hypertension urgencies easy for industrial scale-up.
34481906	2	67	theme	precipitation	323:335	arg1	technique					337:345	nano-cocrystal (NCC) anti-solvent precipitation technique	289:345	nano-cocrystal (NCC) anti-solvent precipitation technique	289:345	The present study describes a soluble form of drug based on nano-cocrystal (NCC) anti-solvent precipitation technique.
34481906	4	68	theme	TA	601:602	arg1	impact					586:591	the impact	582:591	the impact of CAR, TA, poloxamer 188 (stabilizer) concentrations, and anti-solvent/solvent ratio on CAR NCCs particle size (PS)	582:708	Central Composite Design (CCD) considering the impact of CAR, TA, poloxamer 188 (stabilizer) concentrations, and anti-solvent/solvent ratio on CAR NCCs particle size (PS) could produce ultra-fine NCCs (about 1 nm).
34481906	1	69	theme	water	211:215	arg1	solubility					217:226	its very low water solubility	198:226	its very low water solubility	198:226	Carvedilol (CAR) is a strategic beta-blocker agent which its application has been limited by its very low water solubility.
34481906	9	70	theme	NCC	1379:1381	arg1	technology					1383:1392	the NCC technology	1375:1392	the NCC technology	1375:1392	In conclusion, the NCC technology could produce the first safe soluble form of CAR for treating hypertension urgencies easy for industrial scale-up.
34481906	0	71	dep	Design	0:5	arg1	Development					48:58	Development	48:58	Design of ultra-fine carvedilol nanococrystals: Development of a safe and stable injectable formulation.	0:103	Design of ultra-fine carvedilol nanococrystals: Development of a safe and stable injectable formulation.
34481906	1	72	theme	strategic	127:135	arg1	Carvedilol					105:114	Carvedilol	105:114	Carvedilol (CAR)	105:120	Carvedilol (CAR) is a strategic beta-blocker agent which its application has been limited by its very low water solubility.
34481906	1	72	theme	strategic	127:135	arg1	agent					150:154	a strategic beta-blocker agent	125:154	a strategic beta-blocker agent which its application has been limited by its very low water solubility	125:226	Carvedilol (CAR) is a strategic beta-blocker agent which its application has been limited by its very low water solubility.
33483066	6	0	theme	healing	788:794	arg1	potential					796:804	The superior wound healing potential	769:804	The superior wound healing potential	769:804	The superior wound healing potential was ascribed to the enhanced levels of reduced glutathione, glutathione-S-transferase, catalase and decreased lipid peroxidation.
33483066	7	1	theme	epidermal	985:993	arg1	reasons					1126:1132	the reasons	1122:1132	the reasons of improved wound healing	1122:1158	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	7	1	theme	epidermal	985:993	arg1	regulation					1017:1026	down regulation	1012:1026	down regulation	1012:1026	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	7	1	theme	epidermal	985:993	arg1	granulation					972:982	granulation	972:982	granulation	972:982	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	7	1	theme	epidermal	985:993	arg1	regeneration					995:1006	epidermal regeneration	985:1006	epidermal regeneration	985:1006	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	7	1	theme	epidermal	985:993	arg1	angiogenesis					958:969	improved angiogenesis	949:969	improved angiogenesis	949:969	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	4	2	theme	prepared	512:519	arg1	VCF2					549:552	The prepared vancomycin-chitosan film 2 (VCF2)	508:553	The prepared vancomycin-chitosan film 2 (VCF2)	508:553	The prepared vancomycin-chitosan film 2 (VCF2) physically displayed a substantial tensile strength and swelling ratio.
33483066	6	3	theme	lipid	916:920	arg1	peroxidation					922:933	decreased lipid peroxidation	906:933	decreased lipid peroxidation	906:933	The superior wound healing potential was ascribed to the enhanced levels of reduced glutathione, glutathione-S-transferase, catalase and decreased lipid peroxidation.
33483066	7	4	theme	factor	1065:1070	arg1	expressions					1035:1045	the expressions	1031:1045	the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B	1031:1115	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	5	5	theme	wound	737:741	arg1	healing					743:749	improved wound healing	728:749	improved wound healing	728:749	Pharmacologically, VCF2 exhibited sustained vancomycin release, excellent antibacterial activity and improved wound healing efficacy in rats.
33483066	6	6	theme	decreased	906:914	arg1	peroxidation					922:933	decreased lipid peroxidation	906:933	decreased lipid peroxidation	906:933	The superior wound healing potential was ascribed to the enhanced levels of reduced glutathione, glutathione-S-transferase, catalase and decreased lipid peroxidation.
33483066	5	7	theme	improved	728:735	arg1	healing					743:749	improved wound healing	728:749	improved wound healing	728:749	Pharmacologically, VCF2 exhibited sustained vancomycin release, excellent antibacterial activity and improved wound healing efficacy in rats.
33483066	6	8	theme	catalase	893:900	arg1	levels					835:840	the enhanced levels	822:840	the enhanced levels of reduced glutathione, glutathione-S-transferase, catalase and decreased lipid peroxidation	822:933	The superior wound healing potential was ascribed to the enhanced levels of reduced glutathione, glutathione-S-transferase, catalase and decreased lipid peroxidation.
33483066	7	9	from	regulation	1017:1026	arg1	expressions					1035:1045	the expressions	1031:1045	the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B	1031:1115	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	2	10	theme	Current	154:160	arg1	study					162:166	Current study	154:166	Current study	154:166	Current study aimed to fabricate novel chitosan-based composite films of vancomycin for wound healing applications.
33483066	3	11	dep	aureus	447:452	arg1	study					488:492	efficacy study	479:492	efficacy study	479:492	The developed vancomycin-chitosan films were evaluated for various quality attributes and were subjected to anti-bacterial activity against methicillin resistant Staphylococcus aureus (MRSA) and wound healing efficacy study in rat model.
33483066	5	12	theme	sustained	661:669	arg1	release					682:688	sustained vancomycin release	661:688	sustained vancomycin release	661:688	Pharmacologically, VCF2 exhibited sustained vancomycin release, excellent antibacterial activity and improved wound healing efficacy in rats.
33483066	7	13	from	granulation	972:982	arg1	expressions					1035:1045	the expressions	1031:1045	the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B	1031:1115	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	6	14	theme	wound	782:786	arg1	healing					788:794	wound healing	782:794	The superior wound healing potential	769:804	The superior wound healing potential was ascribed to the enhanced levels of reduced glutathione, glutathione-S-transferase, catalase and decreased lipid peroxidation.
33483066	8	15	theme	potential	1275:1283	arg1	approach					1297:1304	a potential therapeutic approach	1273:1304	a potential therapeutic approach in burn wounds	1273:1319	Thus, it is plausible to say that VCF2 could provide a potential therapeutic approach in burn wounds.
33483066	7	16	theme	improved	949:956	arg1	reasons					1126:1132	the reasons	1122:1132	the reasons of improved wound healing	1122:1158	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	7	16	theme	improved	949:956	arg1	regulation					1017:1026	down regulation	1012:1026	down regulation	1012:1026	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	7	16	theme	improved	949:956	arg1	granulation					972:982	granulation	972:982	granulation	972:982	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	7	16	theme	improved	949:956	arg1	regeneration					995:1006	epidermal regeneration	985:1006	epidermal regeneration	985:1006	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	7	16	theme	improved	949:956	arg1	angiogenesis					958:969	improved angiogenesis	949:969	improved angiogenesis	949:969	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	7	17	theme	molecular	1198:1206	arg1	techniques					1208:1217	histopathological and molecular techniques	1176:1217	histopathological and molecular techniques	1176:1217	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	5	18	theme	vancomycin	671:680	arg1	release					682:688	sustained vancomycin release	661:688	sustained vancomycin release	661:688	Pharmacologically, VCF2 exhibited sustained vancomycin release, excellent antibacterial activity and improved wound healing efficacy in rats.
33483066	1	19	theme	Burn	80:83	arg1	form					133:136	the most prevalent and devastating form	98:136	the most prevalent and devastating form of skin trauma	98:151	Burn injuries are the most prevalent and devastating form of skin trauma.
33483066	1	19	theme	Burn	80:83	arg1	injuries					85:92	Burn injuries	80:92	Burn injuries	80:92	Burn injuries are the most prevalent and devastating form of skin trauma.
33483066	6	20	theme	superior	773:780	arg1	potential					796:804	The superior wound healing potential	769:804	The superior wound healing potential	769:804	The superior wound healing potential was ascribed to the enhanced levels of reduced glutathione, glutathione-S-transferase, catalase and decreased lipid peroxidation.
33483066	3	21	theme	efficacy	479:486	arg1	study					488:492	efficacy study	479:492	efficacy study	479:492	The developed vancomycin-chitosan films were evaluated for various quality attributes and were subjected to anti-bacterial activity against methicillin resistant Staphylococcus aureus (MRSA) and wound healing efficacy study in rat model.
33483066	3	22	theme	anti-bacterial	378:391	arg1	activity					393:400	anti-bacterial activity	378:400	anti-bacterial activity against methicillin resistant Staphylococcus aureus (MRSA) and wound healing efficacy study in rat model	378:505	The developed vancomycin-chitosan films were evaluated for various quality attributes and were subjected to anti-bacterial activity against methicillin resistant Staphylococcus aureus (MRSA) and wound healing efficacy study in rat model.
33483066	4	23	theme	film	541:544	arg1	VCF2					549:552	The prepared vancomycin-chitosan film 2 (VCF2)	508:553	The prepared vancomycin-chitosan film 2 (VCF2)	508:553	The prepared vancomycin-chitosan film 2 (VCF2) physically displayed a substantial tensile strength and swelling ratio.
33483066	8	24	theme	burn	1309:1312	arg1	wounds					1314:1319	burn wounds	1309:1319	burn wounds	1309:1319	Thus, it is plausible to say that VCF2 could provide a potential therapeutic approach in burn wounds.
33483066	7	25	theme	tumor	1050:1054	arg1	factor					1065:1070	tumor necrosis factor	1050:1070	tumor necrosis factor	1050:1070	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	4	26	theme	vancomycin-chitosan	521:539	arg1	VCF2					549:552	The prepared vancomycin-chitosan film 2 (VCF2)	508:553	The prepared vancomycin-chitosan film 2 (VCF2)	508:553	The prepared vancomycin-chitosan film 2 (VCF2) physically displayed a substantial tensile strength and swelling ratio.
33483066	5	27	theme	excellent	691:699	arg1	activity					715:722	excellent antibacterial activity	691:722	excellent antibacterial activity	691:722	Pharmacologically, VCF2 exhibited sustained vancomycin release, excellent antibacterial activity and improved wound healing efficacy in rats.
33483066	6	28	theme	glutathione	853:863	arg1	levels					835:840	the enhanced levels	822:840	the enhanced levels of reduced glutathione, glutathione-S-transferase, catalase and decreased lipid peroxidation	822:933	The superior wound healing potential was ascribed to the enhanced levels of reduced glutathione, glutathione-S-transferase, catalase and decreased lipid peroxidation.
33483066	7	29	from	regeneration	995:1006	arg1	expressions					1035:1045	the expressions	1031:1045	the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B	1031:1115	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	7	30	theme	wound	1146:1150	arg1	healing					1152:1158	improved wound healing	1137:1158	improved wound healing	1137:1158	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	3	31	theme	wound	465:469	arg1	healing					471:477	wound healing	465:477	wound healing	465:477	The developed vancomycin-chitosan films were evaluated for various quality attributes and were subjected to anti-bacterial activity against methicillin resistant Staphylococcus aureus (MRSA) and wound healing efficacy study in rat model.
33483066	7	32	theme	healing	1152:1158	arg1	reasons					1126:1132	the reasons	1122:1132	the reasons of improved wound healing	1122:1158	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	7	32	theme	healing	1152:1158	arg1	regulation					1017:1026	down regulation	1012:1026	down regulation	1012:1026	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	7	32	theme	healing	1152:1158	arg1	granulation					972:982	granulation	972:982	granulation	972:982	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	7	32	theme	healing	1152:1158	arg1	regeneration					995:1006	epidermal regeneration	985:1006	epidermal regeneration	985:1006	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	7	32	theme	healing	1152:1158	arg1	angiogenesis					958:969	improved angiogenesis	949:969	improved angiogenesis	949:969	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	5	33	theme	antibacterial	701:713	arg1	activity					715:722	excellent antibacterial activity	691:722	excellent antibacterial activity	691:722	Pharmacologically, VCF2 exhibited sustained vancomycin release, excellent antibacterial activity and improved wound healing efficacy in rats.
33483066	6	34	theme	reduced	845:851	arg1	glutathione					853:863	glutathione	853:863	glutathione	853:863	The superior wound healing potential was ascribed to the enhanced levels of reduced glutathione, glutathione-S-transferase, catalase and decreased lipid peroxidation.
33483066	4	35	theme	tensile	590:596	arg1	strength					598:605	a substantial tensile strength	576:605	a substantial tensile strength	576:605	The prepared vancomycin-chitosan film 2 (VCF2) physically displayed a substantial tensile strength and swelling ratio.
33483066	6	36	theme	glutathione-S-transferase	866:890	arg1	levels					835:840	the enhanced levels	822:840	the enhanced levels of reduced glutathione, glutathione-S-transferase, catalase and decreased lipid peroxidation	822:933	The superior wound healing potential was ascribed to the enhanced levels of reduced glutathione, glutathione-S-transferase, catalase and decreased lipid peroxidation.
33483066	0	37	theme	pharmacological	16:30	arg1	evaluation					32:41	pharmacological evaluation	16:41	pharmacological evaluation	16:41	Development and pharmacological evaluation of vancomycin loaded chitosan films.
33483066	4	38	theme	swelling	611:618	arg1	ratio					620:624	swelling ratio	611:624	swelling ratio	611:624	The prepared vancomycin-chitosan film 2 (VCF2) physically displayed a substantial tensile strength and swelling ratio.
33483066	1	39	theme	prevalent	107:115	arg1	form					133:136	the most prevalent and devastating form	98:136	the most prevalent and devastating form of skin trauma	98:151	Burn injuries are the most prevalent and devastating form of skin trauma.
33483066	1	39	theme	prevalent	107:115	arg1	injuries					85:92	Burn injuries	80:92	Burn injuries	80:92	Burn injuries are the most prevalent and devastating form of skin trauma.
33483066	7	40	theme	necrosis	1056:1063	arg1	factor					1065:1070	tumor necrosis factor	1050:1070	tumor necrosis factor	1050:1070	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	0	41	theme	vancomycin	46:55	arg1	Development					0:10	Development	0:10	Development	0:10	Development and pharmacological evaluation of vancomycin loaded chitosan films.
33483066	0	41	theme	vancomycin	46:55	arg1	evaluation					32:41	pharmacological evaluation	16:41	pharmacological evaluation	16:41	Development and pharmacological evaluation of vancomycin loaded chitosan films.
33483066	3	42	theme	developed	274:282	arg1	films					304:308	The developed vancomycin-chitosan films	270:308	The developed vancomycin-chitosan films	270:308	The developed vancomycin-chitosan films were evaluated for various quality attributes and were subjected to anti-bacterial activity against methicillin resistant Staphylococcus aureus (MRSA) and wound healing efficacy study in rat model.
33483066	2	43	theme	wound	242:246	arg1	healing					248:254	wound healing	242:254	wound healing applications	242:267	Current study aimed to fabricate novel chitosan-based composite films of vancomycin for wound healing applications.
33483066	3	44	theme	vancomycin-chitosan	284:302	arg1	films					304:308	The developed vancomycin-chitosan films	270:308	The developed vancomycin-chitosan films	270:308	The developed vancomycin-chitosan films were evaluated for various quality attributes and were subjected to anti-bacterial activity against methicillin resistant Staphylococcus aureus (MRSA) and wound healing efficacy study in rat model.
33483066	3	45	from	activity	393:400	arg1	model					501:505	rat model	497:505	rat model	497:505	The developed vancomycin-chitosan films were evaluated for various quality attributes and were subjected to anti-bacterial activity against methicillin resistant Staphylococcus aureus (MRSA) and wound healing efficacy study in rat model.
33483066	3	46	theme	rat	497:499	arg1	model					501:505	rat model	497:505	rat model	497:505	The developed vancomycin-chitosan films were evaluated for various quality attributes and were subjected to anti-bacterial activity against methicillin resistant Staphylococcus aureus (MRSA) and wound healing efficacy study in rat model.
33483066	0	47	theme	chitosan	64:71	arg1	films					73:77	chitosan films	64:77	chitosan films	64:77	Development and pharmacological evaluation of vancomycin loaded chitosan films.
33483066	3	48	theme	various	329:335	arg1	attributes					345:354	various quality attributes	329:354	various quality attributes	329:354	The developed vancomycin-chitosan films were evaluated for various quality attributes and were subjected to anti-bacterial activity against methicillin resistant Staphylococcus aureus (MRSA) and wound healing efficacy study in rat model.
33483066	7	49	theme	histopathological	1176:1192	arg1	techniques					1208:1217	histopathological and molecular techniques	1176:1217	histopathological and molecular techniques	1176:1217	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	2	50	theme	vancomycin	227:236	arg1	films					218:222	novel chitosan-based composite films	187:222	novel chitosan-based composite films of vancomycin for wound healing applications	187:267	Current study aimed to fabricate novel chitosan-based composite films of vancomycin for wound healing applications.
33483066	6	51	theme	enhanced	826:833	arg1	levels					835:840	the enhanced levels	822:840	the enhanced levels of reduced glutathione, glutathione-S-transferase, catalase and decreased lipid peroxidation	822:933	The superior wound healing potential was ascribed to the enhanced levels of reduced glutathione, glutathione-S-transferase, catalase and decreased lipid peroxidation.
33483066	4	52	theme	substantial	578:588	arg1	strength					598:605	a substantial tensile strength	576:605	a substantial tensile strength	576:605	The prepared vancomycin-chitosan film 2 (VCF2) physically displayed a substantial tensile strength and swelling ratio.
33483066	3	53	theme	quality	337:343	arg1	attributes					345:354	various quality attributes	329:354	various quality attributes	329:354	The developed vancomycin-chitosan films were evaluated for various quality attributes and were subjected to anti-bacterial activity against methicillin resistant Staphylococcus aureus (MRSA) and wound healing efficacy study in rat model.
33483066	6	54	theme	peroxidation	922:933	arg1	levels					835:840	the enhanced levels	822:840	the enhanced levels of reduced glutathione, glutathione-S-transferase, catalase and decreased lipid peroxidation	822:933	The superior wound healing potential was ascribed to the enhanced levels of reduced glutathione, glutathione-S-transferase, catalase and decreased lipid peroxidation.
33483066	7	55	theme	kappa	1109:1113	arg1	B					1115:1115	nuclear factor kappa B	1094:1115	nuclear factor kappa B	1094:1115	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	1	56	theme	devastating	121:131	arg1	form					133:136	the most prevalent and devastating form	98:136	the most prevalent and devastating form of skin trauma	98:151	Burn injuries are the most prevalent and devastating form of skin trauma.
33483066	1	56	theme	devastating	121:131	arg1	injuries					85:92	Burn injuries	80:92	Burn injuries	80:92	Burn injuries are the most prevalent and devastating form of skin trauma.
33483066	7	57	theme	down	1012:1015	arg1	reasons					1126:1132	the reasons	1122:1132	the reasons of improved wound healing	1122:1158	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	7	57	theme	down	1012:1015	arg1	angiogenesis					958:969	improved angiogenesis	949:969	improved angiogenesis	949:969	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	7	57	theme	down	1012:1015	arg1	granulation					972:982	granulation	972:982	granulation	972:982	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	7	57	theme	down	1012:1015	arg1	regeneration					995:1006	epidermal regeneration	985:1006	epidermal regeneration	985:1006	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	7	57	theme	down	1012:1015	arg1	regulation					1017:1026	down regulation	1012:1026	down regulation	1012:1026	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	7	58	from	angiogenesis	958:969	arg1	expressions					1035:1045	the expressions	1031:1045	the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B	1031:1115	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	8	59	dep	potential	1275:1283	arg1	therapeutic					1285:1295	therapeutic	1285:1295	therapeutic	1285:1295	Thus, it is plausible to say that VCF2 could provide a potential therapeutic approach in burn wounds.
33483066	2	60	theme	composite	208:216	arg1	films					218:222	novel chitosan-based composite films	187:222	novel chitosan-based composite films of vancomycin for wound healing applications	187:267	Current study aimed to fabricate novel chitosan-based composite films of vancomycin for wound healing applications.
33483066	7	61	theme	B	1115:1115	arg1	expressions					1035:1045	the expressions	1031:1045	the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B	1031:1115	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	2	62	theme	chitosan-based	193:206	arg1	films					218:222	novel chitosan-based composite films	187:222	novel chitosan-based composite films of vancomycin for wound healing applications	187:267	Current study aimed to fabricate novel chitosan-based composite films of vancomycin for wound healing applications.
33483066	7	63	theme	nuclear	1094:1100	arg1	B					1115:1115	nuclear factor kappa B	1094:1115	nuclear factor kappa B	1094:1115	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	2	64	theme	novel	187:191	arg1	films					218:222	novel chitosan-based composite films	187:222	novel chitosan-based composite films of vancomycin for wound healing applications	187:267	Current study aimed to fabricate novel chitosan-based composite films of vancomycin for wound healing applications.
33483066	7	65	theme	improved	1137:1144	arg1	healing					1152:1158	improved wound healing	1137:1158	improved wound healing	1137:1158	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	7	66	theme	factor	1102:1107	arg1	B					1115:1115	nuclear factor kappa B	1094:1115	nuclear factor kappa B	1094:1115	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	1	67	theme	skin	141:144	arg1	trauma					146:151	skin trauma	141:151	skin trauma	141:151	Burn injuries are the most prevalent and devastating form of skin trauma.
33483066	5	68	dep	release	682:688	arg1	efficacy					751:758	efficacy	751:758	efficacy	751:758	Pharmacologically, VCF2 exhibited sustained vancomycin release, excellent antibacterial activity and improved wound healing efficacy in rats.
33483066	8	69	from	approach	1297:1304	arg1	wounds					1314:1319	burn wounds	1309:1319	burn wounds	1309:1319	Thus, it is plausible to say that VCF2 could provide a potential therapeutic approach in burn wounds.
33483066	2	70	theme	healing	248:254	arg1	applications					256:267	wound healing applications	242:267	wound healing applications	242:267	Current study aimed to fabricate novel chitosan-based composite films of vancomycin for wound healing applications.
33483066	7	71	theme	cyclooxygenase-2	1073:1088	arg1	expressions					1035:1045	the expressions	1031:1045	the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B	1031:1115	Furthermore, improved angiogenesis, granulation, epidermal regeneration and down regulation in the expressions of tumor necrosis factor, cyclooxygenase-2 and nuclear factor kappa B were the reasons of improved wound healing as confirmed by histopathological and molecular techniques.
33483066	1	72	theme	trauma	146:151	arg1	form					133:136	the most prevalent and devastating form	98:136	the most prevalent and devastating form of skin trauma	98:151	Burn injuries are the most prevalent and devastating form of skin trauma.
33483066	1	72	theme	trauma	146:151	arg1	injuries					85:92	Burn injuries	80:92	Burn injuries	80:92	Burn injuries are the most prevalent and devastating form of skin trauma.
32828406	4	0	from	weaning	738:744	arg1	intake					770:775	sow average daily feed intake	747:775	sow average daily feed intake	747:775	When sows were fed 0.8 % and 1.6 % IN, there was a larger litter weight at weaning, sow average daily feed intake and piglet average daily gain during lactation compared with values for these variables in the CON group (P < 0.05).
32828406	4	0	from	weaning	738:744	arg1	gain					802:805	piglet average daily gain	781:805	piglet average daily gain	781:805	When sows were fed 0.8 % and 1.6 % IN, there was a larger litter weight at weaning, sow average daily feed intake and piglet average daily gain during lactation compared with values for these variables in the CON group (P < 0.05).
32828406	4	0	from	weaning	738:744	arg1	weight					728:733	a larger litter weight	712:733	a larger litter weight at weaning	712:744	When sows were fed 0.8 % and 1.6 % IN, there was a larger litter weight at weaning, sow average daily feed intake and piglet average daily gain during lactation compared with values for these variables in the CON group (P < 0.05).
32828406	2	1	theme	groups：basal	317:328	arg1	diet					330:333	four groups：basal diet	312:333	four groups：basal diet (CON);	312:340	At day 80 of gestation sows were randomly assigned to four groups：basal diet (CON); or basal diet with 0.8 %; 1.6 %; or 2.4 % inulin.
32828406	2	1	theme	groups：basal	317:328	arg1	CON					336:338	CON	336:338	CON	336:338	At day 80 of gestation sows were randomly assigned to four groups：basal diet (CON); or basal diet with 0.8 %; 1.6 %; or 2.4 % inulin.
32828406	5	2	theme	acid	967:970	arg1	concentration					939:951	serum concentration	933:951	serum concentration of free fatty acid, total cholesterol, and high-density lipoprotein cholesterol	933:1031	Additionally, there was an increase in serum concentration of free fatty acid, total cholesterol, and high-density lipoprotein cholesterol with increasing amounts of inulin in the diet (linear, P < 0.05).
32828406	1	3	theme	performance	245:255	arg1	evaluation					134:143	evaluation	134:143	evaluation of effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance	134:255	In this study there was evaluation of effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance.
32828406	5	4	theme	fatty	961:965	arg1	acid					967:970	free fatty acid	956:970	free fatty acid	956:970	Additionally, there was an increase in serum concentration of free fatty acid, total cholesterol, and high-density lipoprotein cholesterol with increasing amounts of inulin in the diet (linear, P < 0.05).
32828406	8	5	theme	pig	1575:1577	arg1	industry					1579:1586	the pig industry	1571:1586	the pig industry	1571:1586	In conclusion, results of the present study indicated feeding inulin during late gestation improved reproductive performance of sows, thus, may be a novel additive for the pig industry in improving efficiency of pork production.
32828406	1	6	theme	inulin	167:172	arg1	effects					148:154	effects	148:154	effects of dietary inulin during late gestation on sow physiology	148:212	In this study there was evaluation of effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance.
32828406	1	6	theme	inulin	167:172	arg1	duration					225:232	farrowing duration	215:232	farrowing duration	215:232	In this study there was evaluation of effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance.
32828406	1	6	theme	inulin	167:172	arg1	performance					245:255	piglet performance	238:255	piglet performance	238:255	In this study there was evaluation of effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance.
32828406	4	7	theme	litter	721:726	arg1	weight					728:733	a larger litter weight	712:733	a larger litter weight at weaning	712:744	When sows were fed 0.8 % and 1.6 % IN, there was a larger litter weight at weaning, sow average daily feed intake and piglet average daily gain during lactation compared with values for these variables in the CON group (P < 0.05).
32828406	1	8	from	performance	245:255	arg1	physiology					203:212	sow physiology	199:212	sow physiology	199:212	In this study there was evaluation of effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance.
32828406	3	9	theme	diet	411:414	arg1	feeding					396:402	The feeding	392:402	The feeding of the diet with 1.6 % inulin	392:432	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	8	10	theme	present	1433:1439	arg1	study					1441:1445	the present study	1429:1445	the present study	1429:1445	In conclusion, results of the present study indicated feeding inulin during late gestation improved reproductive performance of sows, thus, may be a novel additive for the pig industry in improving efficiency of pork production.
32828406	1	11	theme	late	181:184	arg1	gestation					186:194	late gestation	181:194	late gestation	181:194	In this study there was evaluation of effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance.
32828406	7	12	theme	increasing	1350:1359	arg1	inulin					1372:1377	inulin	1372:1377	inulin	1372:1377	Furthermore, there was a linear increase in serum activity of total antioxidant capacity, total superoxide dismutase and glutathione peroxidase with increasing amounts of inulin in the diet (P < 0.05).
32828406	7	12	theme	increasing	1350:1359	arg1	amounts					1361:1367	increasing amounts	1350:1367	increasing amounts of inulin	1350:1377	Furthermore, there was a linear increase in serum activity of total antioxidant capacity, total superoxide dismutase and glutathione peroxidase with increasing amounts of inulin in the diet (P < 0.05).
32828406	8	13	theme	pork	1615:1618	arg1	production					1620:1629	pork production	1615:1629	pork production	1615:1629	In conclusion, results of the present study indicated feeding inulin during late gestation improved reproductive performance of sows, thus, may be a novel additive for the pig industry in improving efficiency of pork production.
32828406	3	14	from	weights	453:459	arg1	birth					478:482	birth	478:482	birth	478:482	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	1	15	from	physiology	203:212	arg1	evaluation					134:143	evaluation	134:143	evaluation of effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance	134:255	In this study there was evaluation of effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance.
32828406	8	16	theme	late	1479:1482	arg1	gestation					1484:1492	late gestation	1479:1492	late gestation improved reproductive performance of sows, thus, may be a novel additive for the pig industry in improving efficiency of pork production	1479:1629	In conclusion, results of the present study indicated feeding inulin during late gestation improved reproductive performance of sows, thus, may be a novel additive for the pig industry in improving efficiency of pork production.
32828406	7	17	theme	superoxide	1297:1306	arg1	increase					1233:1240	a linear increase	1224:1240	a linear increase in serum activity of total antioxidant capacity	1224:1288	Furthermore, there was a linear increase in serum activity of total antioxidant capacity, total superoxide dismutase and glutathione peroxidase with increasing amounts of inulin in the diet (P < 0.05).
32828406	7	17	theme	superoxide	1297:1306	arg1	dismutase					1308:1316	total superoxide dismutase	1291:1316	total superoxide dismutase	1291:1316	Furthermore, there was a linear increase in serum activity of total antioxidant capacity, total superoxide dismutase and glutathione peroxidase with increasing amounts of inulin in the diet (P < 0.05).
32828406	4	18	theme	feed	765:768	arg1	intake					770:775	sow average daily feed intake	747:775	sow average daily feed intake	747:775	When sows were fed 0.8 % and 1.6 % IN, there was a larger litter weight at weaning, sow average daily feed intake and piglet average daily gain during lactation compared with values for these variables in the CON group (P < 0.05).
32828406	0	19	theme	sow	51:53	arg1	physiology					55:64	sow physiology	51:64	sow physiology	51:64	Effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance.
32828406	1	20	from	effects	148:154	arg1	physiology					203:212	sow physiology	199:212	sow physiology	199:212	In this study there was evaluation of effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance.
32828406	5	21	theme	cholesterol	1021:1031	arg1	concentration					939:951	serum concentration	933:951	serum concentration of free fatty acid, total cholesterol, and high-density lipoprotein cholesterol	933:1031	Additionally, there was an increase in serum concentration of free fatty acid, total cholesterol, and high-density lipoprotein cholesterol with increasing amounts of inulin in the diet (linear, P < 0.05).
32828406	1	22	from	evaluation	134:143	arg1	physiology					203:212	sow physiology	199:212	sow physiology	199:212	In this study there was evaluation of effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance.
32828406	7	23	theme	antioxidant	1269:1279	arg1	capacity					1281:1288	total antioxidant capacity	1263:1288	total antioxidant capacity	1263:1288	Furthermore, there was a linear increase in serum activity of total antioxidant capacity, total superoxide dismutase and glutathione peroxidase with increasing amounts of inulin in the diet (P < 0.05).
32828406	0	24	from	Effects	0:6	arg1	physiology					55:64	sow physiology	51:64	sow physiology	51:64	Effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance.
32828406	0	24	from	Effects	0:6	arg1	duration					77:84	farrowing duration	67:84	farrowing duration	67:84	Effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance.
32828406	0	24	from	Effects	0:6	arg1	performance					97:107	piglet performance	90:107	piglet performance	90:107	Effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance.
32828406	5	25	theme	increasing	1038:1047	arg1	inulin					1060:1065	inulin	1060:1065	inulin	1060:1065	Additionally, there was an increase in serum concentration of free fatty acid, total cholesterol, and high-density lipoprotein cholesterol with increasing amounts of inulin in the diet (linear, P < 0.05).
32828406	5	25	theme	increasing	1038:1047	arg1	amounts					1049:1055	increasing amounts	1038:1055	increasing amounts of inulin	1038:1065	Additionally, there was an increase in serum concentration of free fatty acid, total cholesterol, and high-density lipoprotein cholesterol with increasing amounts of inulin in the diet (linear, P < 0.05).
32828406	2	26	dep	%	382:382	arg1	inulin					384:389	inulin	384:389	2.4 % inulin	378:389	At day 80 of gestation sows were randomly assigned to four groups：basal diet (CON); or basal diet with 0.8 %; 1.6 %; or 2.4 % inulin.
32828406	8	27	theme	sows	1531:1534	arg1	additive					1558:1565	additive	1558:1565	additive	1558:1565	In conclusion, results of the present study indicated feeding inulin during late gestation improved reproductive performance of sows, thus, may be a novel additive for the pig industry in improving efficiency of pork production.
32828406	8	27	theme	sows	1531:1534	arg1	performance					1516:1526	improved reproductive performance	1494:1526	improved reproductive performance of sows	1494:1534	In conclusion, results of the present study indicated feeding inulin during late gestation improved reproductive performance of sows, thus, may be a novel additive for the pig industry in improving efficiency of pork production.
32828406	4	28	theme	average	751:757	arg1	intake					770:775	sow average daily feed intake	747:775	sow average daily feed intake	747:775	When sows were fed 0.8 % and 1.6 % IN, there was a larger litter weight at weaning, sow average daily feed intake and piglet average daily gain during lactation compared with values for these variables in the CON group (P < 0.05).
32828406	3	29	from	birth	608:612	arg1	dead					600:603	dead	600:603	dead	600:603	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	2	30	theme	basal	345:349	arg1	diet					351:354	basal diet	345:354	basal diet	345:354	At day 80 of gestation sows were randomly assigned to four groups：basal diet (CON); or basal diet with 0.8 %; 1.6 %; or 2.4 % inulin.
32828406	5	31	theme	total	973:977	arg1	cholesterol					979:989	total cholesterol	973:989	total cholesterol	973:989	Additionally, there was an increase in serum concentration of free fatty acid, total cholesterol, and high-density lipoprotein cholesterol with increasing amounts of inulin in the diet (linear, P < 0.05).
32828406	0	32	theme	late	33:36	arg1	gestation					38:46	late gestation	33:46	late gestation	33:46	Effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance.
32828406	5	33	theme	serum	933:937	arg1	concentration					939:951	serum concentration	933:951	serum concentration of free fatty acid, total cholesterol, and high-density lipoprotein cholesterol	933:1031	Additionally, there was an increase in serum concentration of free fatty acid, total cholesterol, and high-density lipoprotein cholesterol with increasing amounts of inulin in the diet (linear, P < 0.05).
32828406	0	34	theme	dietary	11:17	arg1	inulin					19:24	dietary inulin	11:24	dietary inulin during late gestation	11:46	Effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance.
32828406	7	35	theme	inulin	1372:1377	arg1	inulin					1372:1377	inulin	1372:1377	inulin	1372:1377	Furthermore, there was a linear increase in serum activity of total antioxidant capacity, total superoxide dismutase and glutathione peroxidase with increasing amounts of inulin in the diet (P < 0.05).
32828406	7	35	theme	inulin	1372:1377	arg1	amounts					1361:1367	increasing amounts	1350:1367	increasing amounts of inulin	1350:1377	Furthermore, there was a linear increase in serum activity of total antioxidant capacity, total superoxide dismutase and glutathione peroxidase with increasing amounts of inulin in the diet (P < 0.05).
32828406	4	36	theme	average	788:794	arg1	gain					802:805	piglet average daily gain	781:805	piglet average daily gain	781:805	When sows were fed 0.8 % and 1.6 % IN, there was a larger litter weight at weaning, sow average daily feed intake and piglet average daily gain during lactation compared with values for these variables in the CON group (P < 0.05).
32828406	3	37	theme	lesser	528:533	arg1	duration					494:501	a shorter duration	484:501	a shorter duration of the farrowing period	484:525	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	3	37	theme	lesser	528:533	arg1	interval					549:556	lesser average birth interval	528:556	lesser average birth interval between piglets	528:572	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	7	38	theme	linear	1226:1231	arg1	increase					1233:1240	a linear increase	1224:1240	a linear increase in serum activity of total antioxidant capacity	1224:1288	Furthermore, there was a linear increase in serum activity of total antioxidant capacity, total superoxide dismutase and glutathione peroxidase with increasing amounts of inulin in the diet (P < 0.05).
32828406	7	38	theme	linear	1226:1231	arg1	dismutase					1308:1316	total superoxide dismutase	1291:1316	total superoxide dismutase	1291:1316	Furthermore, there was a linear increase in serum activity of total antioxidant capacity, total superoxide dismutase and glutathione peroxidase with increasing amounts of inulin in the diet (P < 0.05).
32828406	7	38	theme	linear	1226:1231	arg1	peroxidase					1334:1343	glutathione peroxidase	1322:1343	glutathione peroxidase	1322:1343	Furthermore, there was a linear increase in serum activity of total antioxidant capacity, total superoxide dismutase and glutathione peroxidase with increasing amounts of inulin in the diet (P < 0.05).
32828406	3	39	theme	period	520:525	arg1	duration					494:501	a shorter duration	484:501	a shorter duration of the farrowing period	484:525	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	3	39	theme	period	520:525	arg1	number					582:587	lesser number	575:587	lesser number of piglets	575:598	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	3	39	theme	period	520:525	arg1	interval					549:556	lesser average birth interval	528:556	lesser average birth interval between piglets	528:572	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	6	40	theme	serum	1129:1133	arg1	concentrations					1135:1148	greater serum concentrations	1121:1148	greater serum concentrations of glucose	1121:1159	Sows fed 1.6 % IN had greater serum concentrations of glucose than those in the CON group (P < 0.05).
32828406	8	41	theme	improved	1494:1501	arg1	additive					1558:1565	additive	1558:1565	additive	1558:1565	In conclusion, results of the present study indicated feeding inulin during late gestation improved reproductive performance of sows, thus, may be a novel additive for the pig industry in improving efficiency of pork production.
32828406	8	41	theme	improved	1494:1501	arg1	performance					1516:1526	improved reproductive performance	1494:1526	improved reproductive performance of sows	1494:1534	In conclusion, results of the present study indicated feeding inulin during late gestation improved reproductive performance of sows, thus, may be a novel additive for the pig industry in improving efficiency of pork production.
32828406	5	42	theme	cholesterol	979:989	arg1	concentration					939:951	serum concentration	933:951	serum concentration of free fatty acid, total cholesterol, and high-density lipoprotein cholesterol	933:1031	Additionally, there was an increase in serum concentration of free fatty acid, total cholesterol, and high-density lipoprotein cholesterol with increasing amounts of inulin in the diet (linear, P < 0.05).
32828406	3	43	theme	birth	543:547	arg1	duration					494:501	a shorter duration	484:501	a shorter duration of the farrowing period	484:525	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	3	43	theme	birth	543:547	arg1	interval					549:556	lesser average birth interval	528:556	lesser average birth interval between piglets	528:572	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	5	44	from	increase	921:928	arg1	concentration					939:951	serum concentration	933:951	serum concentration of free fatty acid, total cholesterol, and high-density lipoprotein cholesterol	933:1031	Additionally, there was an increase in serum concentration of free fatty acid, total cholesterol, and high-density lipoprotein cholesterol with increasing amounts of inulin in the diet (linear, P < 0.05).
32828406	1	45	theme	duration	225:232	arg1	evaluation					134:143	evaluation	134:143	evaluation of effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance	134:255	In this study there was evaluation of effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance.
32828406	6	46	theme	CON	1179:1181	arg1	P <					1190:1192	P < 0.05	1190:1197	P < 0.05	1190:1197	Sows fed 1.6 % IN had greater serum concentrations of glucose than those in the CON group (P < 0.05).
32828406	6	46	theme	CON	1179:1181	arg1	group					1183:1187	the CON group	1175:1187	the CON group (P < 0.05)	1175:1198	Sows fed 1.6 % IN had greater serum concentrations of glucose than those in the CON group (P < 0.05).
32828406	1	47	theme	piglet	238:243	arg1	performance					245:255	piglet performance	238:255	piglet performance	238:255	In this study there was evaluation of effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance.
32828406	7	48	from	increase	1233:1240	arg1	activity					1251:1258	serum activity	1245:1258	serum activity of total antioxidant capacity	1245:1288	Furthermore, there was a linear increase in serum activity of total antioxidant capacity, total superoxide dismutase and glutathione peroxidase with increasing amounts of inulin in the diet (P < 0.05).
32828406	6	49	contain	had	1117:1119	arg1	Sows					1099:1102	Sows	1099:1102	Sows fed 1.6 % IN	1099:1115	Sows fed 1.6 % IN had greater serum concentrations of glucose than those in the CON group (P < 0.05).
32828406	6	49	contain	had	1117:1119	arg2	concentrations					1135:1148	greater serum concentrations	1121:1148	greater serum concentrations of glucose	1121:1159	Sows fed 1.6 % IN had greater serum concentrations of glucose than those in the CON group (P < 0.05).
32828406	3	50	theme	%	425:425	arg1	inulin					427:432	1.6 % inulin	421:432	1.6 % inulin	421:432	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	1	51	theme	effects	148:154	arg1	evaluation					134:143	evaluation	134:143	evaluation of effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance	134:255	In this study there was evaluation of effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance.
32828406	3	52	theme	piglets	592:598	arg1	duration					494:501	a shorter duration	484:501	a shorter duration of the farrowing period	484:525	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	3	52	theme	piglets	592:598	arg1	number					582:587	lesser number	575:587	lesser number of piglets	575:598	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	3	53	with	feeding	396:402	arg1	inulin					427:432	1.6 % inulin	421:432	1.6 % inulin	421:432	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	1	54	theme	dietary	159:165	arg1	inulin					167:172	dietary inulin	159:172	dietary inulin during late gestation	159:194	In this study there was evaluation of effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance.
32828406	2	55	theme	gestation	271:279	arg1	day					261:263	day 80	261:266	day 80 of gestation	261:279	At day 80 of gestation sows were randomly assigned to four groups：basal diet (CON); or basal diet with 0.8 %; 1.6 %; or 2.4 % inulin.
32828406	4	56	theme	larger	714:719	arg1	weight					728:733	a larger litter weight	712:733	a larger litter weight at weaning	712:744	When sows were fed 0.8 % and 1.6 % IN, there was a larger litter weight at weaning, sow average daily feed intake and piglet average daily gain during lactation compared with values for these variables in the CON group (P < 0.05).
32828406	1	57	from	duration	225:232	arg1	physiology					203:212	sow physiology	199:212	sow physiology	199:212	In this study there was evaluation of effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance.
32828406	7	58	theme	glutathione	1322:1332	arg1	increase					1233:1240	a linear increase	1224:1240	a linear increase in serum activity of total antioxidant capacity	1224:1288	Furthermore, there was a linear increase in serum activity of total antioxidant capacity, total superoxide dismutase and glutathione peroxidase with increasing amounts of inulin in the diet (P < 0.05).
32828406	7	58	theme	glutathione	1322:1332	arg1	peroxidase					1334:1343	glutathione peroxidase	1322:1343	glutathione peroxidase	1322:1343	Furthermore, there was a linear increase in serum activity of total antioxidant capacity, total superoxide dismutase and glutathione peroxidase with increasing amounts of inulin in the diet (P < 0.05).
32828406	8	59	theme	production	1620:1629	arg1	efficiency					1601:1610	efficiency	1601:1610	efficiency of pork production	1601:1629	In conclusion, results of the present study indicated feeding inulin during late gestation improved reproductive performance of sows, thus, may be a novel additive for the pig industry in improving efficiency of pork production.
32828406	3	60	theme	litter	468:473	arg1	weights					453:459	larger weights	446:459	larger weights of the litter at birth	446:482	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	8	61	theme	study	1441:1445	arg1	results					1418:1424	results	1418:1424	results of the present study	1418:1445	In conclusion, results of the present study indicated feeding inulin during late gestation improved reproductive performance of sows, thus, may be a novel additive for the pig industry in improving efficiency of pork production.
32828406	8	62	theme	feeding	1457:1463	arg1	inulin					1465:1470	feeding inulin	1457:1470	feeding inulin	1457:1470	In conclusion, results of the present study indicated feeding inulin during late gestation improved reproductive performance of sows, thus, may be a novel additive for the pig industry in improving efficiency of pork production.
32828406	3	63	theme	larger	446:451	arg1	weights					453:459	larger weights	446:459	larger weights of the litter at birth	446:482	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	7	64	theme	total	1291:1295	arg1	increase					1233:1240	a linear increase	1224:1240	a linear increase in serum activity of total antioxidant capacity	1224:1288	Furthermore, there was a linear increase in serum activity of total antioxidant capacity, total superoxide dismutase and glutathione peroxidase with increasing amounts of inulin in the diet (P < 0.05).
32828406	7	64	theme	total	1291:1295	arg1	dismutase					1308:1316	total superoxide dismutase	1291:1316	total superoxide dismutase	1291:1316	Furthermore, there was a linear increase in serum activity of total antioxidant capacity, total superoxide dismutase and glutathione peroxidase with increasing amounts of inulin in the diet (P < 0.05).
32828406	5	65	theme	inulin	1060:1065	arg1	inulin					1060:1065	inulin	1060:1065	inulin	1060:1065	Additionally, there was an increase in serum concentration of free fatty acid, total cholesterol, and high-density lipoprotein cholesterol with increasing amounts of inulin in the diet (linear, P < 0.05).
32828406	5	65	theme	inulin	1060:1065	arg1	amounts					1049:1055	increasing amounts	1038:1055	increasing amounts of inulin	1038:1065	Additionally, there was an increase in serum concentration of free fatty acid, total cholesterol, and high-density lipoprotein cholesterol with increasing amounts of inulin in the diet (linear, P < 0.05).
32828406	6	66	theme	greater	1121:1127	arg1	concentrations					1135:1148	greater serum concentrations	1121:1148	greater serum concentrations of glucose	1121:1159	Sows fed 1.6 % IN had greater serum concentrations of glucose than those in the CON group (P < 0.05).
32828406	3	67	theme	shorter	486:492	arg1	duration					494:501	a shorter duration	484:501	a shorter duration of the farrowing period	484:525	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	3	67	theme	shorter	486:492	arg1	number					582:587	lesser number	575:587	lesser number of piglets	575:598	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	3	67	theme	shorter	486:492	arg1	interval					549:556	lesser average birth interval	528:556	lesser average birth interval between piglets	528:572	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	4	68	from	variables	855:863	arg1	P <					883:885	P < 0.05	883:890	P < 0.05	883:890	When sows were fed 0.8 % and 1.6 % IN, there was a larger litter weight at weaning, sow average daily feed intake and piglet average daily gain during lactation compared with values for these variables in the CON group (P < 0.05).
32828406	4	68	from	variables	855:863	arg1	group					876:880	the CON group	868:880	the CON group (P < 0.05)	868:891	When sows were fed 0.8 % and 1.6 % IN, there was a larger litter weight at weaning, sow average daily feed intake and piglet average daily gain during lactation compared with values for these variables in the CON group (P < 0.05).
32828406	7	69	theme	capacity	1281:1288	arg1	activity					1251:1258	serum activity	1245:1258	serum activity of total antioxidant capacity	1245:1288	Furthermore, there was a linear increase in serum activity of total antioxidant capacity, total superoxide dismutase and glutathione peroxidase with increasing amounts of inulin in the diet (P < 0.05).
32828406	4	70	theme	daily	759:763	arg1	intake					770:775	sow average daily feed intake	747:775	sow average daily feed intake	747:775	When sows were fed 0.8 % and 1.6 % IN, there was a larger litter weight at weaning, sow average daily feed intake and piglet average daily gain during lactation compared with values for these variables in the CON group (P < 0.05).
32828406	1	71	theme	sow	199:201	arg1	physiology					203:212	sow physiology	199:212	sow physiology	199:212	In this study there was evaluation of effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance.
32828406	5	72	theme	lipoprotein	1009:1019	arg1	cholesterol					1021:1031	high-density lipoprotein cholesterol	996:1031	high-density lipoprotein cholesterol	996:1031	Additionally, there was an increase in serum concentration of free fatty acid, total cholesterol, and high-density lipoprotein cholesterol with increasing amounts of inulin in the diet (linear, P < 0.05).
32828406	3	73	theme	P	652:652	arg1	<					654:654	P < 0.05	652:659	P < 0.05	652:659	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	3	73	theme	P	652:652	arg1	birth					645:649	birth	645:649	birth (P < 0.05)	645:660	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	0	74	theme	farrowing	67:75	arg1	duration					77:84	farrowing duration	67:84	farrowing duration	67:84	Effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance.
32828406	7	75	theme	total	1263:1267	arg1	capacity					1281:1288	total antioxidant capacity	1263:1288	total antioxidant capacity	1263:1288	Furthermore, there was a linear increase in serum activity of total antioxidant capacity, total superoxide dismutase and glutathione peroxidase with increasing amounts of inulin in the diet (P < 0.05).
32828406	8	76	theme	reproductive	1503:1514	arg1	additive					1558:1565	additive	1558:1565	additive	1558:1565	In conclusion, results of the present study indicated feeding inulin during late gestation improved reproductive performance of sows, thus, may be a novel additive for the pig industry in improving efficiency of pork production.
32828406	8	76	theme	reproductive	1503:1514	arg1	performance					1516:1526	improved reproductive performance	1494:1526	improved reproductive performance of sows	1494:1534	In conclusion, results of the present study indicated feeding inulin during late gestation improved reproductive performance of sows, thus, may be a novel additive for the pig industry in improving efficiency of pork production.
32828406	3	77	from	birth	645:649	arg1	dead					637:640	dead	637:640	dead	637:640	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	3	78	dep	dead	637:640	arg1	fewer					619:623	fewer	619:623	fewer	619:623	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	4	79	theme	sow	747:749	arg1	intake					770:775	sow average daily feed intake	747:775	sow average daily feed intake	747:775	When sows were fed 0.8 % and 1.6 % IN, there was a larger litter weight at weaning, sow average daily feed intake and piglet average daily gain during lactation compared with values for these variables in the CON group (P < 0.05).
32828406	0	80	theme	inulin	19:24	arg1	Effects					0:6	Effects	0:6	Effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance.	0:108	Effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance.
32828406	3	81	from	birth	478:482	arg1	weights					453:459	larger weights	446:459	larger weights of the litter at birth	446:482	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	3	81	from	birth	478:482	arg1	litter					468:473	the litter	464:473	the litter at birth	464:482	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	6	82	theme	glucose	1153:1159	arg1	concentrations					1135:1148	greater serum concentrations	1121:1148	greater serum concentrations of glucose	1121:1159	Sows fed 1.6 % IN had greater serum concentrations of glucose than those in the CON group (P < 0.05).
32828406	5	83	theme	free	956:959	arg1	acid					967:970	free fatty acid	956:970	free fatty acid	956:970	Additionally, there was an increase in serum concentration of free fatty acid, total cholesterol, and high-density lipoprotein cholesterol with increasing amounts of inulin in the diet (linear, P < 0.05).
32828406	7	84	theme	serum	1245:1249	arg1	activity					1251:1258	serum activity	1245:1258	serum activity of total antioxidant capacity	1245:1288	Furthermore, there was a linear increase in serum activity of total antioxidant capacity, total superoxide dismutase and glutathione peroxidase with increasing amounts of inulin in the diet (P < 0.05).
32828406	4	85	theme	piglet	781:786	arg1	gain					802:805	piglet average daily gain	781:805	piglet average daily gain	781:805	When sows were fed 0.8 % and 1.6 % IN, there was a larger litter weight at weaning, sow average daily feed intake and piglet average daily gain during lactation compared with values for these variables in the CON group (P < 0.05).
32828406	3	86	theme	farrowing	510:518	arg1	period					520:525	the farrowing period	506:525	the farrowing period	506:525	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	0	87	theme	piglet	90:95	arg1	performance					97:107	piglet performance	90:107	piglet performance	90:107	Effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance.
32828406	4	88	theme	daily	796:800	arg1	gain					802:805	piglet average daily gain	781:805	piglet average daily gain	781:805	When sows were fed 0.8 % and 1.6 % IN, there was a larger litter weight at weaning, sow average daily feed intake and piglet average daily gain during lactation compared with values for these variables in the CON group (P < 0.05).
32828406	8	89	dep	gestation	1484:1492	arg1	additive					1558:1565	additive	1558:1565	additive	1558:1565	In conclusion, results of the present study indicated feeding inulin during late gestation improved reproductive performance of sows, thus, may be a novel additive for the pig industry in improving efficiency of pork production.
32828406	8	89	dep	gestation	1484:1492	arg1	performance					1516:1526	improved reproductive performance	1494:1526	improved reproductive performance of sows	1494:1534	In conclusion, results of the present study indicated feeding inulin during late gestation improved reproductive performance of sows, thus, may be a novel additive for the pig industry in improving efficiency of pork production.
32828406	3	90	theme	average	535:541	arg1	duration					494:501	a shorter duration	484:501	a shorter duration of the farrowing period	484:525	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	3	90	theme	average	535:541	arg1	interval					549:556	lesser average birth interval	528:556	lesser average birth interval between piglets	528:572	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	5	91	dep	linear	1080:1085	arg1	P <					1088:1090	P < 0.05	1088:1095	P < 0.05	1088:1095	Additionally, there was an increase in serum concentration of free fatty acid, total cholesterol, and high-density lipoprotein cholesterol with increasing amounts of inulin in the diet (linear, P < 0.05).
32828406	4	92	theme	CON	872:874	arg1	P <					883:885	P < 0.05	883:890	P < 0.05	883:890	When sows were fed 0.8 % and 1.6 % IN, there was a larger litter weight at weaning, sow average daily feed intake and piglet average daily gain during lactation compared with values for these variables in the CON group (P < 0.05).
32828406	4	92	theme	CON	872:874	arg1	group					876:880	the CON group	868:880	the CON group (P < 0.05)	868:891	When sows were fed 0.8 % and 1.6 % IN, there was a larger litter weight at weaning, sow average daily feed intake and piglet average daily gain during lactation compared with values for these variables in the CON group (P < 0.05).
32828406	3	93	theme	lesser	575:580	arg1	duration					494:501	a shorter duration	484:501	a shorter duration of the farrowing period	484:525	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	3	93	theme	lesser	575:580	arg1	number					582:587	lesser number	575:587	lesser number of piglets	575:598	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	1	94	theme	farrowing	215:223	arg1	duration					225:232	farrowing duration	215:232	farrowing duration	215:232	In this study there was evaluation of effects of dietary inulin during late gestation on sow physiology, farrowing duration and piglet performance.
32828406	3	95	theme	1.6	421:423	arg1	%					425:425	%	425:425	%	425:425	The feeding of the diet with 1.6 % inulin resulted in larger weights of the litter at birth a shorter duration of the farrowing period, lesser average birth interval between piglets, lesser number of piglets dead at birth, and fewer piglets/sow dead at birth (P < 0.05).
32828406	5	96	theme	high-density	996:1007	arg1	cholesterol					1021:1031	high-density lipoprotein cholesterol	996:1031	high-density lipoprotein cholesterol	996:1031	Additionally, there was an increase in serum concentration of free fatty acid, total cholesterol, and high-density lipoprotein cholesterol with increasing amounts of inulin in the diet (linear, P < 0.05).
33480834	9	0	theme	Vibrio	1541:1546	arg1	species					1520:1526	a novel species	1512:1526	a novel species	1512:1526	On the basis of evidence from the present polyphasic study, strain SM6T is considered to represent a novel species of the genus Vibrio, for which the name Vibrio agarilyticus sp.
33480834	4	1	theme	closest	541:547	arg1	relatives					562:570	The closest phylogenetic relatives	537:570	The closest phylogenetic relatives of SM6T	537:578	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	4	1	theme	closest	541:547	arg1	MSSRF60T					606:613	Vibrio plantisponsor MSSRF60T	585:613	Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity)	585:666	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	7	2	theme	genomic	1129:1135	arg1	sequences					1137:1145	the assembled genomic sequences	1115:1145	the assembled genomic sequences	1115:1145	The DNA G+C content of the assembled genomic sequences was 47.37 % for strain SM6T.
33480834	11	3	theme	1K04327	1653:1659	arg1	T					1661:1661	=KCTC 82076T=MCCC 1K04327 T	1635:1661	=KCTC 82076T=MCCC 1K04327 T	1635:1661	The type strain is SM6T (=KCTC 82076T=MCCC 1K04327 T).
33480834	11	3	theme	1K04327	1653:1659	arg1	SM6T					1629:1632	SM6T	1629:1632	SM6T (=KCTC 82076T=MCCC 1K04327 T)	1629:1662	The type strain is SM6T (=KCTC 82076T=MCCC 1K04327 T).
33480834	4	4	theme	Vibrio	669:674	arg1	%					703:703	97.27 %	697:703	97.27 %	697:703	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	4	4	theme	Vibrio	669:674	arg1	R-40492T					687:694	Vibrio variabilis R-40492T	669:694	Vibrio variabilis R-40492T (97.27 %)	669:704	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	4	5	theme	16S	624:626	arg1	similarity					656:665	97.38 % 16S rRNA gene sequence pairwise similarity	616:665	97.38 % 16S rRNA gene sequence pairwise similarity	616:665	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	4	5	theme	16S	624:626	arg1	MSSRF60T					606:613	Vibrio plantisponsor MSSRF60T	585:613	Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity)	585:666	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	3	6	theme	16S	355:357	arg1	sequences					369:377	16S rRNA gene sequences	355:377	16S rRNA gene sequences	355:377	Phylogenetic analysis based on 16S rRNA gene sequences, multilocus sequence analysis, phylogenomic analysis of single-copy gene families and whole genome data showed that strain SM6T belonged to the genus Vibrio.
33480834	6	7	dep	feature	1048:1054	arg1	C18					1059:1061	C18	1059:1061	C18 : 1 ω7c or/and C18 : 1 ω6c	1059:1088	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1 ω7c or/and C16 : 1 ω6c), C16 : 0 and summed feature 8 (C18 : 1 ω7c or/and C18 : 1 ω6c).
33480834	9	8	theme	Vibrio	1568:1573	arg1	sp					1588:1589	the name Vibrio agarilyticus sp	1559:1589	the name Vibrio agarilyticus sp	1559:1589	On the basis of evidence from the present polyphasic study, strain SM6T is considered to represent a novel species of the genus Vibrio, for which the name Vibrio agarilyticus sp.
33480834	4	9	theme	gene	633:636	arg1	similarity					656:665	97.38 % 16S rRNA gene sequence pairwise similarity	616:665	97.38 % 16S rRNA gene sequence pairwise similarity	616:665	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	4	9	theme	gene	633:636	arg1	MSSRF60T					606:613	Vibrio plantisponsor MSSRF60T	585:613	Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity)	585:666	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	7	10	theme	DNA	1096:1098	arg1	content					1104:1110	The DNA G+C content	1092:1110	The DNA G+C content of the assembled genomic sequences	1092:1145	The DNA G+C content of the assembled genomic sequences was 47.37 % for strain SM6T.
33480834	7	10	theme	DNA	1096:1098	arg1	%					1157:1157	47.37 %	1151:1157	47.37 %	1151:1157	The DNA G+C content of the assembled genomic sequences was 47.37 % for strain SM6T.
33480834	3	11	theme	genus	523:527	arg1	Vibrio					529:534	the genus Vibrio	519:534	the genus Vibrio	519:534	Phylogenetic analysis based on 16S rRNA gene sequences, multilocus sequence analysis, phylogenomic analysis of single-copy gene families and whole genome data showed that strain SM6T belonged to the genus Vibrio.
33480834	2	12	theme	marine	212:217	arg1	bacterium					219:227	A Gram-strain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacterium	128:227	A Gram-strain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacterium	128:227	A Gram-strain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacterium, designated SM6T, was isolated from surface seawater collected in Daya Bay (Guangdong, China).
33480834	6	13	theme	or/and	1008:1013	arg1	C16					1015:1017	1 ω7c or/and C16	1002:1017	1 ω7c or/and C16 : 1 ω6c	1002:1025	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1 ω7c or/and C16 : 1 ω6c), C16 : 0 and summed feature 8 (C18 : 1 ω7c or/and C18 : 1 ω6c).
33480834	6	14	dep	C16	996:998	arg1	C16					1015:1017	1 ω7c or/and C16	1002:1017	1 ω7c or/and C16 : 1 ω6c	1002:1025	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1 ω7c or/and C16 : 1 ω6c), C16 : 0 and summed feature 8 (C18 : 1 ω7c or/and C18 : 1 ω6c).
33480834	2	15	attach	isolated	251:258	arg2	bacterium					219:227	A Gram-strain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacterium	128:227	A Gram-strain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacterium	128:227	A Gram-strain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacterium, designated SM6T, was isolated from surface seawater collected in Daya Bay (Guangdong, China).
33480834	2	15	attach	isolated	251:258	arg1	seawater					273:280	surface seawater	265:280	surface seawater collected in Daya Bay (Guangdong, China)	265:321	A Gram-strain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacterium, designated SM6T, was isolated from surface seawater collected in Daya Bay (Guangdong, China).
33480834	6	16	theme	summed	1041:1046	arg1	feature					1048:1054	summed feature 8	1041:1056	summed feature 8 (C18 : 1 ω7c or/and C18 : 1 ω6c)	1041:1089	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1 ω7c or/and C16 : 1 ω6c), C16 : 0 and summed feature 8 (C18 : 1 ω7c or/and C18 : 1 ω6c).
33480834	11	17	theme	82076T=MCCC	1641:1651	arg1	T					1661:1661	=KCTC 82076T=MCCC 1K04327 T	1635:1661	=KCTC 82076T=MCCC 1K04327 T	1635:1661	The type strain is SM6T (=KCTC 82076T=MCCC 1K04327 T).
33480834	11	17	theme	82076T=MCCC	1641:1651	arg1	SM6T					1629:1632	SM6T	1629:1632	SM6T (=KCTC 82076T=MCCC 1K04327 T)	1629:1662	The type strain is SM6T (=KCTC 82076T=MCCC 1K04327 T).
33480834	5	18	theme	optimum	835:841	arg1	°C					831:832	10-45 °C	825:832	10-45 °C (optimum 30 °C)	825:848	Growth of strain SM6T occurred at 10-45 °C (optimum 30 °C), at pH 6.0-9.0 (optimum 6.0) and in the presence of 0-10 % (w/v) NaCl (optimum 3-8 %).
33480834	5	18	theme	optimum	835:841	arg1	°C					846:847	optimum 30 °C	835:847	optimum 30 °C	835:847	Growth of strain SM6T occurred at 10-45 °C (optimum 30 °C), at pH 6.0-9.0 (optimum 6.0) and in the presence of 0-10 % (w/v) NaCl (optimum 3-8 %).
33480834	4	19	theme	pairwise	647:654	arg1	similarity					656:665	97.38 % 16S rRNA gene sequence pairwise similarity	616:665	97.38 % 16S rRNA gene sequence pairwise similarity	616:665	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	4	19	theme	pairwise	647:654	arg1	MSSRF60T					606:613	Vibrio plantisponsor MSSRF60T	585:613	Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity)	585:666	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	5	20	theme	SM6T	808:811	arg1	Growth					791:796	Growth	791:796	Growth of strain SM6T	791:811	Growth of strain SM6T occurred at 10-45 °C (optimum 30 °C), at pH 6.0-9.0 (optimum 6.0) and in the presence of 0-10 % (w/v) NaCl (optimum 3-8 %).
33480834	8	21	theme	nucleotide	1184:1193	arg1	values					1204:1209	Average nucleotide identity values	1176:1209	Average nucleotide identity values between SM6T and its reference species	1176:1248	Average nucleotide identity values between SM6T and its reference species were lower than the threshold for species delineation (95-96 %); in silico DNA-DNA hybridization further showed that the strains shared less than 70 % similarity.
33480834	4	22	theme	Vibrio	707:712	arg1	%					746:746	97.21 %	740:746	97.21 %	740:746	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	4	22	theme	Vibrio	707:712	arg1	ATCC					727:730	Vibrio aestuarianus ATCC 35048T	707:737	Vibrio aestuarianus ATCC 35048T (97.21 %)	707:747	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	1	23	dep	bacterium	55:63	arg1	nov.					24:27	nov.	24:27	nov.	24:27	nov., an agar-digesting marine bacterium isolated from coastal seawater in Daya Bay (Guangdong, China).
33480834	9	24	theme	polyphasic	1455:1464	arg1	study					1466:1470	the present polyphasic study	1443:1470	the present polyphasic study	1443:1470	On the basis of evidence from the present polyphasic study, strain SM6T is considered to represent a novel species of the genus Vibrio, for which the name Vibrio agarilyticus sp.
33480834	8	25	dep	similarity	1401:1410	arg1	%					1399:1399	%	1399:1399	%	1399:1399	Average nucleotide identity values between SM6T and its reference species were lower than the threshold for species delineation (95-96 %); in silico DNA-DNA hybridization further showed that the strains shared less than 70 % similarity.
33480834	4	26	theme	phylogenetic	549:560	arg1	relatives					562:570	The closest phylogenetic relatives	537:570	The closest phylogenetic relatives of SM6T	537:578	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	4	26	theme	phylogenetic	549:560	arg1	MSSRF60T					606:613	Vibrio plantisponsor MSSRF60T	585:613	Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity)	585:666	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	3	27	theme	multilocus	380:389	arg1	analysis					400:407	multilocus sequence analysis	380:407	multilocus sequence analysis	380:407	Phylogenetic analysis based on 16S rRNA gene sequences, multilocus sequence analysis, phylogenomic analysis of single-copy gene families and whole genome data showed that strain SM6T belonged to the genus Vibrio.
33480834	0	28	theme	Vibrio	0:5	arg1	sp					20:21	Vibrio agarilyticus sp	0:21	Vibrio agarilyticus sp.	0:22	Vibrio agarilyticus sp.
33480834	3	29	theme	phylogenomic	410:421	arg1	analysis					423:430	phylogenomic analysis	410:430	phylogenomic analysis of single-copy gene families	410:459	Phylogenetic analysis based on 16S rRNA gene sequences, multilocus sequence analysis, phylogenomic analysis of single-copy gene families and whole genome data showed that strain SM6T belonged to the genus Vibrio.
33480834	3	30	theme	rRNA	359:362	arg1	sequences					369:377	16S rRNA gene sequences	355:377	16S rRNA gene sequences	355:377	Phylogenetic analysis based on 16S rRNA gene sequences, multilocus sequence analysis, phylogenomic analysis of single-copy gene families and whole genome data showed that strain SM6T belonged to the genus Vibrio.
33480834	2	31	theme	Gram-strain-negative	130:149	arg1	bacterium					219:227	A Gram-strain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacterium	128:227	A Gram-strain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacterium	128:227	A Gram-strain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacterium, designated SM6T, was isolated from surface seawater collected in Daya Bay (Guangdong, China).
33480834	4	32	theme	sagamiensis	760:770	arg1	LC2-047T					772:779	Vibrio sagamiensis LC2-047T	753:779	Vibrio sagamiensis LC2-047T (97.3 %)	753:788	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	4	32	theme	sagamiensis	760:770	arg1	%					787:787	97.3 %	782:787	97.3 %	782:787	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	6	33	theme	fatty	953:957	arg1	%					970:970	>10 %	966:970	>10 %	966:970	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1 ω7c or/and C16 : 1 ω6c), C16 : 0 and summed feature 8 (C18 : 1 ω7c or/and C18 : 1 ω6c).
33480834	6	33	theme	fatty	953:957	arg1	acids					959:963	The predominant fatty acids	937:963	The predominant fatty acids (>10 %)	937:971	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1 ω7c or/and C16 : 1 ω6c), C16 : 0 and summed feature 8 (C18 : 1 ω7c or/and C18 : 1 ω6c).
33480834	9	34	theme	novel	1514:1518	arg1	species					1520:1526	a novel species	1512:1526	a novel species	1512:1526	On the basis of evidence from the present polyphasic study, strain SM6T is considered to represent a novel species of the genus Vibrio, for which the name Vibrio agarilyticus sp.
33480834	3	35	theme	gene	447:450	arg1	families					452:459	single-copy gene families	435:459	single-copy gene families	435:459	Phylogenetic analysis based on 16S rRNA gene sequences, multilocus sequence analysis, phylogenomic analysis of single-copy gene families and whole genome data showed that strain SM6T belonged to the genus Vibrio.
33480834	8	36	theme	DNA-DNA	1325:1331	arg1	hybridization					1333:1345	in silico DNA-DNA hybridization	1315:1345	in silico DNA-DNA hybridization	1315:1345	Average nucleotide identity values between SM6T and its reference species were lower than the threshold for species delineation (95-96 %); in silico DNA-DNA hybridization further showed that the strains shared less than 70 % similarity.
33480834	11	37	theme	=KCTC	1635:1639	arg1	T					1661:1661	=KCTC 82076T=MCCC 1K04327 T	1635:1661	=KCTC 82076T=MCCC 1K04327 T	1635:1661	The type strain is SM6T (=KCTC 82076T=MCCC 1K04327 T).
33480834	11	37	theme	=KCTC	1635:1639	arg1	SM6T					1629:1632	SM6T	1629:1632	SM6T (=KCTC 82076T=MCCC 1K04327 T)	1629:1662	The type strain is SM6T (=KCTC 82076T=MCCC 1K04327 T).
33480834	1	38	from	seawater	87:94	arg1	Bay					104:106	Bay	104:106	Bay	104:106	nov., an agar-digesting marine bacterium isolated from coastal seawater in Daya Bay (Guangdong, China).
33480834	1	38	from	seawater	87:94	arg1	China					120:124	China	120:124	China	120:124	nov., an agar-digesting marine bacterium isolated from coastal seawater in Daya Bay (Guangdong, China).
33480834	1	39	theme	agar-digesting	33:46	arg1	bacterium					55:63	an agar-digesting marine bacterium	30:63	an agar-digesting marine bacterium isolated from coastal seawater in Daya Bay (Guangdong, China)	30:125	nov., an agar-digesting marine bacterium isolated from coastal seawater in Daya Bay (Guangdong, China).
33480834	9	40	theme	genus	1535:1539	arg1	Vibrio					1541:1546	the genus Vibrio	1531:1546	the genus Vibrio	1531:1546	On the basis of evidence from the present polyphasic study, strain SM6T is considered to represent a novel species of the genus Vibrio, for which the name Vibrio agarilyticus sp.
33480834	3	41	theme	genome	471:476	arg1	data					478:481	whole genome data	465:481	whole genome data	465:481	Phylogenetic analysis based on 16S rRNA gene sequences, multilocus sequence analysis, phylogenomic analysis of single-copy gene families and whole genome data showed that strain SM6T belonged to the genus Vibrio.
33480834	7	42	theme	strain	1163:1168	arg1	SM6T					1170:1173	strain SM6T	1163:1173	strain SM6T	1163:1173	The DNA G+C content of the assembled genomic sequences was 47.37 % for strain SM6T.
33480834	2	43	theme	surface	265:271	arg1	seawater					273:280	surface seawater	265:280	surface seawater collected in Daya Bay (Guangdong, China)	265:321	A Gram-strain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacterium, designated SM6T, was isolated from surface seawater collected in Daya Bay (Guangdong, China).
33480834	7	44	theme	sequences	1137:1145	arg1	content					1104:1110	The DNA G+C content	1092:1110	The DNA G+C content of the assembled genomic sequences	1092:1145	The DNA G+C content of the assembled genomic sequences was 47.37 % for strain SM6T.
33480834	7	44	theme	sequences	1137:1145	arg1	%					1157:1157	47.37 %	1151:1157	47.37 %	1151:1157	The DNA G+C content of the assembled genomic sequences was 47.37 % for strain SM6T.
33480834	8	45	theme	in	1315:1316	arg1	hybridization					1333:1345	in silico DNA-DNA hybridization	1315:1345	in silico DNA-DNA hybridization	1315:1345	Average nucleotide identity values between SM6T and its reference species were lower than the threshold for species delineation (95-96 %); in silico DNA-DNA hybridization further showed that the strains shared less than 70 % similarity.
33480834	3	46	theme	strain	495:500	arg1	SM6T					502:505	strain SM6T	495:505	strain SM6T	495:505	Phylogenetic analysis based on 16S rRNA gene sequences, multilocus sequence analysis, phylogenomic analysis of single-copy gene families and whole genome data showed that strain SM6T belonged to the genus Vibrio.
33480834	7	47	theme	assembled	1119:1127	arg1	sequences					1137:1145	the assembled genomic sequences	1115:1145	the assembled genomic sequences	1115:1145	The DNA G+C content of the assembled genomic sequences was 47.37 % for strain SM6T.
33480834	9	48	theme	name	1563:1566	arg1	sp					1588:1589	the name Vibrio agarilyticus sp	1559:1589	the name Vibrio agarilyticus sp	1559:1589	On the basis of evidence from the present polyphasic study, strain SM6T is considered to represent a novel species of the genus Vibrio, for which the name Vibrio agarilyticus sp.
33480834	4	49	theme	%	622:622	arg1	similarity					656:665	97.38 % 16S rRNA gene sequence pairwise similarity	616:665	97.38 % 16S rRNA gene sequence pairwise similarity	616:665	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	4	49	theme	%	622:622	arg1	MSSRF60T					606:613	Vibrio plantisponsor MSSRF60T	585:613	Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity)	585:666	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	9	50	theme	agarilyticus	1575:1586	arg1	sp					1588:1589	the name Vibrio agarilyticus sp	1559:1589	the name Vibrio agarilyticus sp	1559:1589	On the basis of evidence from the present polyphasic study, strain SM6T is considered to represent a novel species of the genus Vibrio, for which the name Vibrio agarilyticus sp.
33480834	4	51	theme	rRNA	628:631	arg1	similarity					656:665	97.38 % 16S rRNA gene sequence pairwise similarity	616:665	97.38 % 16S rRNA gene sequence pairwise similarity	616:665	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	4	51	theme	rRNA	628:631	arg1	MSSRF60T					606:613	Vibrio plantisponsor MSSRF60T	585:613	Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity)	585:666	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	6	52	theme	ω7c	1067:1069	arg1	ω6c					1086:1088	1 ω7c or/and C18 : 1 ω6c	1065:1088	C18 : 1 ω7c or/and C18 : 1 ω6c	1059:1088	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1 ω7c or/and C16 : 1 ω6c), C16 : 0 and summed feature 8 (C18 : 1 ω7c or/and C18 : 1 ω6c).
33480834	7	53	theme	G+C	1100:1102	arg1	content					1104:1110	The DNA G+C content	1092:1110	The DNA G+C content of the assembled genomic sequences	1092:1145	The DNA G+C content of the assembled genomic sequences was 47.37 % for strain SM6T.
33480834	7	53	theme	G+C	1100:1102	arg1	%					1157:1157	47.37 %	1151:1157	47.37 %	1151:1157	The DNA G+C content of the assembled genomic sequences was 47.37 % for strain SM6T.
33480834	4	54	theme	plantisponsor	592:604	arg1	similarity					656:665	97.38 % 16S rRNA gene sequence pairwise similarity	616:665	97.38 % 16S rRNA gene sequence pairwise similarity	616:665	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	4	54	theme	plantisponsor	592:604	arg1	relatives					562:570	The closest phylogenetic relatives	537:570	The closest phylogenetic relatives of SM6T	537:578	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	4	54	theme	plantisponsor	592:604	arg1	MSSRF60T					606:613	Vibrio plantisponsor MSSRF60T	585:613	Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity)	585:666	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	1	55	attach	isolated	65:72	arg1	seawater					87:94	coastal seawater	79:94	coastal seawater in Daya Bay (Guangdong, China)	79:125	nov., an agar-digesting marine bacterium isolated from coastal seawater in Daya Bay (Guangdong, China).
33480834	1	55	attach	isolated	65:72	arg2	bacterium					55:63	an agar-digesting marine bacterium	30:63	an agar-digesting marine bacterium isolated from coastal seawater in Daya Bay (Guangdong, China)	30:125	nov., an agar-digesting marine bacterium isolated from coastal seawater in Daya Bay (Guangdong, China).
33480834	5	56	theme	NaCl	915:918	arg1	presence					890:897	the presence	886:897	the presence of 0-10 % (w/v) NaCl (optimum 3-8 %)	886:934	Growth of strain SM6T occurred at 10-45 °C (optimum 30 °C), at pH 6.0-9.0 (optimum 6.0) and in the presence of 0-10 % (w/v) NaCl (optimum 3-8 %).
33480834	6	57	dep	C18	1059:1061	arg1	ω6c					1086:1088	1 ω7c or/and C18 : 1 ω6c	1065:1088	C18 : 1 ω7c or/and C18 : 1 ω6c	1059:1088	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1 ω7c or/and C16 : 1 ω6c), C16 : 0 and summed feature 8 (C18 : 1 ω7c or/and C18 : 1 ω6c).
33480834	6	58	dep	feature	985:991	arg1	C16					996:998	C16	996:998	C16	996:998	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1 ω7c or/and C16 : 1 ω6c), C16 : 0 and summed feature 8 (C18 : 1 ω7c or/and C18 : 1 ω6c).
33480834	5	59	theme	optimum	921:927	arg1	NaCl					915:918	0-10 % (w/v) NaCl	902:918	0-10 % (w/v) NaCl (optimum 3-8 %)	902:934	Growth of strain SM6T occurred at 10-45 °C (optimum 30 °C), at pH 6.0-9.0 (optimum 6.0) and in the presence of 0-10 % (w/v) NaCl (optimum 3-8 %).
33480834	5	59	theme	optimum	921:927	arg1	%					933:933	optimum 3-8 %	921:933	optimum 3-8 %	921:933	Growth of strain SM6T occurred at 10-45 °C (optimum 30 °C), at pH 6.0-9.0 (optimum 6.0) and in the presence of 0-10 % (w/v) NaCl (optimum 3-8 %).
33480834	2	60	theme	flagellated	200:210	arg1	bacterium					219:227	A Gram-strain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacterium	128:227	A Gram-strain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacterium	128:227	A Gram-strain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacterium, designated SM6T, was isolated from surface seawater collected in Daya Bay (Guangdong, China).
33480834	9	61	dep	evidence	1429:1436	arg1	basis					1420:1424	basis	1420:1424	basis	1420:1424	On the basis of evidence from the present polyphasic study, strain SM6T is considered to represent a novel species of the genus Vibrio, for which the name Vibrio agarilyticus sp.
33480834	9	61	dep	evidence	1429:1436	arg1	the					1416:1418	the	1416:1418	the	1416:1418	On the basis of evidence from the present polyphasic study, strain SM6T is considered to represent a novel species of the genus Vibrio, for which the name Vibrio agarilyticus sp.
33480834	6	62	theme	ω7c	1004:1006	arg1	C16					1015:1017	1 ω7c or/and C16	1002:1017	1 ω7c or/and C16 : 1 ω6c	1002:1025	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1 ω7c or/and C16 : 1 ω6c), C16 : 0 and summed feature 8 (C18 : 1 ω7c or/and C18 : 1 ω6c).
33480834	5	63	theme	%	907:907	arg1	NaCl					915:918	0-10 % (w/v) NaCl	902:918	0-10 % (w/v) NaCl (optimum 3-8 %)	902:934	Growth of strain SM6T occurred at 10-45 °C (optimum 30 °C), at pH 6.0-9.0 (optimum 6.0) and in the presence of 0-10 % (w/v) NaCl (optimum 3-8 %).
33480834	5	63	theme	%	907:907	arg1	%					933:933	optimum 3-8 %	921:933	optimum 3-8 %	921:933	Growth of strain SM6T occurred at 10-45 °C (optimum 30 °C), at pH 6.0-9.0 (optimum 6.0) and in the presence of 0-10 % (w/v) NaCl (optimum 3-8 %).
33480834	8	64	theme	Average	1176:1182	arg1	values					1204:1209	Average nucleotide identity values	1176:1209	Average nucleotide identity values between SM6T and its reference species	1176:1248	Average nucleotide identity values between SM6T and its reference species were lower than the threshold for species delineation (95-96 %); in silico DNA-DNA hybridization further showed that the strains shared less than 70 % similarity.
33480834	4	65	theme	variabilis	676:685	arg1	%					703:703	97.27 %	697:703	97.27 %	697:703	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	4	65	theme	variabilis	676:685	arg1	R-40492T					687:694	Vibrio variabilis R-40492T	669:694	Vibrio variabilis R-40492T (97.27 %)	669:704	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	5	66	theme	w/v	910:912	arg1	NaCl					915:918	0-10 % (w/v) NaCl	902:918	0-10 % (w/v) NaCl (optimum 3-8 %)	902:934	Growth of strain SM6T occurred at 10-45 °C (optimum 30 °C), at pH 6.0-9.0 (optimum 6.0) and in the presence of 0-10 % (w/v) NaCl (optimum 3-8 %).
33480834	5	66	theme	w/v	910:912	arg1	%					933:933	optimum 3-8 %	921:933	optimum 3-8 %	921:933	Growth of strain SM6T occurred at 10-45 °C (optimum 30 °C), at pH 6.0-9.0 (optimum 6.0) and in the presence of 0-10 % (w/v) NaCl (optimum 3-8 %).
33480834	11	67	theme	type	1614:1617	arg1	strain					1619:1624	The type strain	1610:1624	The type strain	1610:1624	The type strain is SM6T (=KCTC 82076T=MCCC 1K04327 T).
33480834	11	67	theme	type	1614:1617	arg1	SM6T					1629:1632	SM6T	1629:1632	SM6T (=KCTC 82076T=MCCC 1K04327 T)	1629:1662	The type strain is SM6T (=KCTC 82076T=MCCC 1K04327 T).
33480834	5	68	theme	strain	801:806	arg1	SM6T					808:811	strain SM6T	801:811	strain SM6T	801:811	Growth of strain SM6T occurred at 10-45 °C (optimum 30 °C), at pH 6.0-9.0 (optimum 6.0) and in the presence of 0-10 % (w/v) NaCl (optimum 3-8 %).
33480834	8	69	theme	identity	1195:1202	arg1	values					1204:1209	Average nucleotide identity values	1176:1209	Average nucleotide identity values between SM6T and its reference species	1176:1248	Average nucleotide identity values between SM6T and its reference species were lower than the threshold for species delineation (95-96 %); in silico DNA-DNA hybridization further showed that the strains shared less than 70 % similarity.
33480834	4	70	theme	sequence	638:645	arg1	similarity					656:665	97.38 % 16S rRNA gene sequence pairwise similarity	616:665	97.38 % 16S rRNA gene sequence pairwise similarity	616:665	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	4	70	theme	sequence	638:645	arg1	MSSRF60T					606:613	Vibrio plantisponsor MSSRF60T	585:613	Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity)	585:666	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	9	71	theme	present	1447:1453	arg1	study					1466:1470	the present polyphasic study	1443:1470	the present polyphasic study	1443:1470	On the basis of evidence from the present polyphasic study, strain SM6T is considered to represent a novel species of the genus Vibrio, for which the name Vibrio agarilyticus sp.
33480834	8	72	dep	in	1315:1316	arg1	silico					1318:1323	silico	1318:1323	silico	1318:1323	Average nucleotide identity values between SM6T and its reference species were lower than the threshold for species delineation (95-96 %); in silico DNA-DNA hybridization further showed that the strains shared less than 70 % similarity.
33480834	6	73	dep	C16	1015:1017	arg1	ω6c					1023:1025	1 ω6c	1021:1025	1 ω7c or/and C16 : 1 ω6c	1002:1025	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1 ω7c or/and C16 : 1 ω6c), C16 : 0 and summed feature 8 (C18 : 1 ω7c or/and C18 : 1 ω6c).
33480834	1	74	theme	marine	48:53	arg1	bacterium					55:63	an agar-digesting marine bacterium	30:63	an agar-digesting marine bacterium isolated from coastal seawater in Daya Bay (Guangdong, China)	30:125	nov., an agar-digesting marine bacterium isolated from coastal seawater in Daya Bay (Guangdong, China).
33480834	3	75	theme	Phylogenetic	324:335	arg1	analysis					337:344	Phylogenetic analysis	324:344	Phylogenetic analysis based on 16S rRNA gene sequences	324:377	Phylogenetic analysis based on 16S rRNA gene sequences, multilocus sequence analysis, phylogenomic analysis of single-copy gene families and whole genome data showed that strain SM6T belonged to the genus Vibrio.
33480834	6	76	theme	C18	1078:1080	arg1	ω6c					1086:1088	1 ω7c or/and C18 : 1 ω6c	1065:1088	C18 : 1 ω7c or/and C18 : 1 ω6c	1059:1088	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1 ω7c or/and C16 : 1 ω6c), C16 : 0 and summed feature 8 (C18 : 1 ω7c or/and C18 : 1 ω6c).
33480834	8	77	theme	reference	1232:1240	arg1	species					1242:1248	its reference species	1228:1248	its reference species	1228:1248	Average nucleotide identity values between SM6T and its reference species were lower than the threshold for species delineation (95-96 %); in silico DNA-DNA hybridization further showed that the strains shared less than 70 % similarity.
33480834	8	78	dep	%	1399:1399	arg1	70 					1396:1398	70 	1396:1398	70 	1396:1398	Average nucleotide identity values between SM6T and its reference species were lower than the threshold for species delineation (95-96 %); in silico DNA-DNA hybridization further showed that the strains shared less than 70 % similarity.
33480834	4	79	theme	aestuarianus	714:725	arg1	%					746:746	97.21 %	740:746	97.21 %	740:746	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	4	79	theme	aestuarianus	714:725	arg1	ATCC					727:730	Vibrio aestuarianus ATCC 35048T	707:737	Vibrio aestuarianus ATCC 35048T (97.21 %)	707:747	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	0	80	theme	agarilyticus	7:18	arg1	sp					20:21	Vibrio agarilyticus sp	0:21	Vibrio agarilyticus sp.	0:22	Vibrio agarilyticus sp.
33480834	1	81	theme	coastal	79:85	arg1	seawater					87:94	coastal seawater	79:94	coastal seawater in Daya Bay (Guangdong, China)	79:125	nov., an agar-digesting marine bacterium isolated from coastal seawater in Daya Bay (Guangdong, China).
33480834	9	82	theme	strain	1473:1478	arg1	SM6T					1480:1483	strain SM6T	1473:1483	strain SM6T	1473:1483	On the basis of evidence from the present polyphasic study, strain SM6T is considered to represent a novel species of the genus Vibrio, for which the name Vibrio agarilyticus sp.
33480834	3	83	theme	sequence	391:398	arg1	analysis					400:407	multilocus sequence analysis	380:407	multilocus sequence analysis	380:407	Phylogenetic analysis based on 16S rRNA gene sequences, multilocus sequence analysis, phylogenomic analysis of single-copy gene families and whole genome data showed that strain SM6T belonged to the genus Vibrio.
33480834	2	84	theme	anaerobic	166:174	arg1	bacterium					219:227	A Gram-strain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacterium	128:227	A Gram-strain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacterium	128:227	A Gram-strain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacterium, designated SM6T, was isolated from surface seawater collected in Daya Bay (Guangdong, China).
33480834	3	85	theme	single-copy	435:445	arg1	families					452:459	single-copy gene families	435:459	single-copy gene families	435:459	Phylogenetic analysis based on 16S rRNA gene sequences, multilocus sequence analysis, phylogenomic analysis of single-copy gene families and whole genome data showed that strain SM6T belonged to the genus Vibrio.
33480834	9	86	from	study	1466:1470	arg1	evidence					1429:1436	evidence	1429:1436	evidence from the present polyphasic study	1429:1470	On the basis of evidence from the present polyphasic study, strain SM6T is considered to represent a novel species of the genus Vibrio, for which the name Vibrio agarilyticus sp.
33480834	6	87	theme	or/and	1071:1076	arg1	ω6c					1086:1088	1 ω7c or/and C18 : 1 ω6c	1065:1088	C18 : 1 ω7c or/and C18 : 1 ω6c	1059:1088	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1 ω7c or/and C16 : 1 ω6c), C16 : 0 and summed feature 8 (C18 : 1 ω7c or/and C18 : 1 ω6c).
33480834	3	88	theme	gene	364:367	arg1	sequences					369:377	16S rRNA gene sequences	355:377	16S rRNA gene sequences	355:377	Phylogenetic analysis based on 16S rRNA gene sequences, multilocus sequence analysis, phylogenomic analysis of single-copy gene families and whole genome data showed that strain SM6T belonged to the genus Vibrio.
33480834	8	89	theme	species	1284:1290	arg1	delineation					1292:1302	species delineation	1284:1302	species delineation (95-96 %)	1284:1312	Average nucleotide identity values between SM6T and its reference species were lower than the threshold for species delineation (95-96 %); in silico DNA-DNA hybridization further showed that the strains shared less than 70 % similarity.
33480834	8	89	theme	species	1284:1290	arg1	%					1311:1311	95-96 %	1305:1311	95-96 %	1305:1311	Average nucleotide identity values between SM6T and its reference species were lower than the threshold for species delineation (95-96 %); in silico DNA-DNA hybridization further showed that the strains shared less than 70 % similarity.
33480834	2	90	theme	rod-shaped	185:194	arg1	bacterium					219:227	A Gram-strain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacterium	128:227	A Gram-strain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacterium	128:227	A Gram-strain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacterium, designated SM6T, was isolated from surface seawater collected in Daya Bay (Guangdong, China).
33480834	4	91	theme	Vibrio	753:758	arg1	LC2-047T					772:779	Vibrio sagamiensis LC2-047T	753:779	Vibrio sagamiensis LC2-047T (97.3 %)	753:788	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	4	91	theme	Vibrio	753:758	arg1	%					787:787	97.3 %	782:787	97.3 %	782:787	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	3	92	theme	whole	465:469	arg1	data					478:481	whole genome data	465:481	whole genome data	465:481	Phylogenetic analysis based on 16S rRNA gene sequences, multilocus sequence analysis, phylogenomic analysis of single-copy gene families and whole genome data showed that strain SM6T belonged to the genus Vibrio.
33480834	6	93	theme	predominant	941:951	arg1	%					970:970	>10 %	966:970	>10 %	966:970	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1 ω7c or/and C16 : 1 ω6c), C16 : 0 and summed feature 8 (C18 : 1 ω7c or/and C18 : 1 ω6c).
33480834	6	93	theme	predominant	941:951	arg1	acids					959:963	The predominant fatty acids	937:963	The predominant fatty acids (>10 %)	937:971	The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1 ω7c or/and C16 : 1 ω6c), C16 : 0 and summed feature 8 (C18 : 1 ω7c or/and C18 : 1 ω6c).
33480834	4	94	theme	SM6T	575:578	arg1	relatives					562:570	The closest phylogenetic relatives	537:570	The closest phylogenetic relatives of SM6T	537:578	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	4	94	theme	SM6T	575:578	arg1	MSSRF60T					606:613	Vibrio plantisponsor MSSRF60T	585:613	Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity)	585:666	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	2	95	theme	motile	177:182	arg1	bacterium					219:227	A Gram-strain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacterium	128:227	A Gram-strain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacterium	128:227	A Gram-strain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacterium, designated SM6T, was isolated from surface seawater collected in Daya Bay (Guangdong, China).
33480834	3	96	theme	families	452:459	arg1	analysis					400:407	multilocus sequence analysis	380:407	multilocus sequence analysis	380:407	Phylogenetic analysis based on 16S rRNA gene sequences, multilocus sequence analysis, phylogenomic analysis of single-copy gene families and whole genome data showed that strain SM6T belonged to the genus Vibrio.
33480834	3	96	theme	families	452:459	arg1	analysis					423:430	phylogenomic analysis	410:430	phylogenomic analysis of single-copy gene families	410:459	Phylogenetic analysis based on 16S rRNA gene sequences, multilocus sequence analysis, phylogenomic analysis of single-copy gene families and whole genome data showed that strain SM6T belonged to the genus Vibrio.
33480834	3	96	theme	families	452:459	arg1	analysis					337:344	Phylogenetic analysis	324:344	Phylogenetic analysis based on 16S rRNA gene sequences	324:377	Phylogenetic analysis based on 16S rRNA gene sequences, multilocus sequence analysis, phylogenomic analysis of single-copy gene families and whole genome data showed that strain SM6T belonged to the genus Vibrio.
33480834	3	96	theme	families	452:459	arg1	data					478:481	whole genome data	465:481	whole genome data	465:481	Phylogenetic analysis based on 16S rRNA gene sequences, multilocus sequence analysis, phylogenomic analysis of single-copy gene families and whole genome data showed that strain SM6T belonged to the genus Vibrio.
33480834	4	97	theme	Vibrio	585:590	arg1	similarity					656:665	97.38 % 16S rRNA gene sequence pairwise similarity	616:665	97.38 % 16S rRNA gene sequence pairwise similarity	616:665	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	4	97	theme	Vibrio	585:590	arg1	relatives					562:570	The closest phylogenetic relatives	537:570	The closest phylogenetic relatives of SM6T	537:578	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
33480834	4	97	theme	Vibrio	585:590	arg1	MSSRF60T					606:613	Vibrio plantisponsor MSSRF60T	585:613	Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity)	585:666	The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %).
32487393	2	0	from	concentration	440:452	arg1	elastic					411:417	elastic	411:417	elastic	411:417	By increasing the concentration of the protein isolate, chitosan hydrogels were more elastic at a protein isolate concentration not exceeding 15% (w/w).
32487393	0	1	dep	In	133:134	arg1	vitro					136:140	vitro	136:140	vitro	136:140	A novel blue crab chitosan/protein composite hydrogel enriched with carotenoids endowed with distinguished wound healing capability: In vitro characterization and in vivo assessment.
32487393	1	2	theme	composite	250:258	arg1	hydrogels					260:268	chitosan and protein isolate composite hydrogels	221:268	chitosan and protein isolate composite hydrogels	221:268	This work aimed to the development of chitosan and protein isolate composite hydrogels, for carotenoids-controlled delivery and wound healing.
32487393	8	3	theme	wound	1285:1289	arg1	healing					1291:1297	wound healing	1285:1297	wound healing	1285:1297	Topical application of hydrogel-based patches allowed the acceleration of wound healing and the complete healing, for composite hydrogel enriched with carotenoids.
32487393	7	4	dep	in	1135:1136	arg1	vivo					1138:1141	vivo	1138:1141	vivo	1138:1141	The in vivo healing potential of hydrogels in rats' models was further studied.
32487393	2	5	theme	protein	365:371	arg1	isolate					373:379	the protein isolate	361:379	the protein isolate	361:379	By increasing the concentration of the protein isolate, chitosan hydrogels were more elastic at a protein isolate concentration not exceeding 15% (w/w).
32487393	1	6	theme	hydrogels	260:268	arg1	development					206:216	the development	202:216	the development of chitosan and protein isolate composite hydrogels	202:268	This work aimed to the development of chitosan and protein isolate composite hydrogels, for carotenoids-controlled delivery and wound healing.
32487393	0	7	theme	wound	107:111	arg1	healing					113:119	distinguished wound healing	93:119	distinguished wound healing capability	93:130	A novel blue crab chitosan/protein composite hydrogel enriched with carotenoids endowed with distinguished wound healing capability: In vitro characterization and in vivo assessment.
32487393	8	8	theme	healing	1316:1322	arg1	acceleration					1269:1280	the acceleration	1265:1280	the acceleration of wound healing and the complete healing	1265:1322	Topical application of hydrogel-based patches allowed the acceleration of wound healing and the complete healing, for composite hydrogel enriched with carotenoids.
32487393	0	9	with	endowed	80:86	arg1	capability					121:130	distinguished wound healing capability	93:130	distinguished wound healing capability	93:130	A novel blue crab chitosan/protein composite hydrogel enriched with carotenoids endowed with distinguished wound healing capability: In vitro characterization and in vivo assessment.
32487393	3	10	theme	osteosarcoma	564:575	arg1	cells					577:581	MG-63 osteosarcoma cells	558:581	MG-63 osteosarcoma cells	558:581	Chitosan-protein isolate composite hydrogels revealed low cytotoxicity towards MG-63 osteosarcoma cells.
32487393	6	11	theme	interesting	1096:1106	arg1	abilities					1120:1128	interesting antioxidant abilities	1096:1128	interesting antioxidant abilities	1096:1128	Thus, developed hydrogels could be applied as pH-sensitive intelligent carriers, for drugs-controlled release, with interesting antioxidant abilities.
32487393	4	12	theme	swelling	622:629	arg1	properties					657:666	its appropriate structural, swelling and mechanical resistance properties	594:666	its appropriate structural, swelling and mechanical resistance properties	594:666	Thanks to its appropriate structural, swelling and mechanical resistance properties, chitosan hydrogel (3%; w/v), reinforced with 15% (w/w) of protein isolate, was selected for the carotenoids in vitro release study.
32487393	0	13	theme	distinguished	93:105	arg1	healing					113:119	distinguished wound healing	93:119	distinguished wound healing capability	93:130	A novel blue crab chitosan/protein composite hydrogel enriched with carotenoids endowed with distinguished wound healing capability: In vitro characterization and in vivo assessment.
32487393	8	14	theme	complete	1307:1314	arg1	healing					1316:1322	the complete healing	1303:1322	the complete healing	1303:1322	Topical application of hydrogel-based patches allowed the acceleration of wound healing and the complete healing, for composite hydrogel enriched with carotenoids.
32487393	2	15	theme	isolate	373:379	arg1	concentration					344:356	the concentration	340:356	the concentration of the protein isolate	340:379	By increasing the concentration of the protein isolate, chitosan hydrogels were more elastic at a protein isolate concentration not exceeding 15% (w/w).
32487393	6	16	theme	drugs-controlled	1065:1080	arg1	release					1082:1088	drugs-controlled release	1065:1088	drugs-controlled release	1065:1088	Thus, developed hydrogels could be applied as pH-sensitive intelligent carriers, for drugs-controlled release, with interesting antioxidant abilities.
32487393	7	17	theme	in	1135:1136	arg1	potential					1151:1159	The in vivo healing potential	1131:1159	The in vivo healing potential of hydrogels in rats' models	1131:1188	The in vivo healing potential of hydrogels in rats' models was further studied.
32487393	6	18	theme	pH-sensitive	1026:1037	arg1	hydrogels					996:1004	developed hydrogels	986:1004	developed hydrogels	986:1004	Thus, developed hydrogels could be applied as pH-sensitive intelligent carriers, for drugs-controlled release, with interesting antioxidant abilities.
32487393	6	18	theme	pH-sensitive	1026:1037	arg1	carriers					1051:1058	pH-sensitive intelligent carriers	1026:1058	pH-sensitive intelligent carriers	1026:1058	Thus, developed hydrogels could be applied as pH-sensitive intelligent carriers, for drugs-controlled release, with interesting antioxidant abilities.
32487393	0	19	theme	healing	113:119	arg1	capability					121:130	distinguished wound healing capability	93:130	distinguished wound healing capability	93:130	A novel blue crab chitosan/protein composite hydrogel enriched with carotenoids endowed with distinguished wound healing capability: In vitro characterization and in vivo assessment.
32487393	4	20	theme	structural	610:619	arg1	properties					657:666	its appropriate structural, swelling and mechanical resistance properties	594:666	its appropriate structural, swelling and mechanical resistance properties	594:666	Thanks to its appropriate structural, swelling and mechanical resistance properties, chitosan hydrogel (3%; w/v), reinforced with 15% (w/w) of protein isolate, was selected for the carotenoids in vitro release study.
32487393	3	21	theme	MG-63	558:562	arg1	cells					577:581	MG-63 osteosarcoma cells	558:581	MG-63 osteosarcoma cells	558:581	Chitosan-protein isolate composite hydrogels revealed low cytotoxicity towards MG-63 osteosarcoma cells.
32487393	6	22	theme	intelligent	1039:1049	arg1	hydrogels					996:1004	developed hydrogels	986:1004	developed hydrogels	986:1004	Thus, developed hydrogels could be applied as pH-sensitive intelligent carriers, for drugs-controlled release, with interesting antioxidant abilities.
32487393	6	22	theme	intelligent	1039:1049	arg1	carriers					1051:1058	pH-sensitive intelligent carriers	1026:1058	pH-sensitive intelligent carriers	1026:1058	Thus, developed hydrogels could be applied as pH-sensitive intelligent carriers, for drugs-controlled release, with interesting antioxidant abilities.
32487393	4	23	dep	in	777:778	arg1	vitro					780:784	vitro	780:784	vitro	780:784	Thanks to its appropriate structural, swelling and mechanical resistance properties, chitosan hydrogel (3%; w/v), reinforced with 15% (w/w) of protein isolate, was selected for the carotenoids in vitro release study.
32487393	0	24	theme	novel	2:6	arg1	crab					13:16	A novel blue crab	0:16	A novel blue crab chitosan/protein composite hydrogel	0:52	A novel blue crab chitosan/protein composite hydrogel enriched with carotenoids endowed with distinguished wound healing capability: In vitro characterization and in vivo assessment.
32487393	3	25	theme	low	533:535	arg1	cytotoxicity					537:548	low cytotoxicity	533:548	low cytotoxicity towards MG-63 osteosarcoma cells	533:581	Chitosan-protein isolate composite hydrogels revealed low cytotoxicity towards MG-63 osteosarcoma cells.
32487393	8	26	theme	Topical	1211:1217	arg1	application					1219:1229	Topical application	1211:1229	Topical application of hydrogel-based patches	1211:1255	Topical application of hydrogel-based patches allowed the acceleration of wound healing and the complete healing, for composite hydrogel enriched with carotenoids.
32487393	7	27	theme	healing	1143:1149	arg1	potential					1151:1159	The in vivo healing potential	1131:1159	The in vivo healing potential of hydrogels in rats' models	1131:1188	The in vivo healing potential of hydrogels in rats' models was further studied.
32487393	1	28	theme	carotenoids-controlled	275:296	arg1	delivery					298:305	carotenoids-controlled delivery	275:305	carotenoids-controlled delivery	275:305	This work aimed to the development of chitosan and protein isolate composite hydrogels, for carotenoids-controlled delivery and wound healing.
32487393	8	29	theme	composite	1329:1337	arg1	hydrogel					1339:1346	composite hydrogel	1329:1346	composite hydrogel enriched with carotenoids	1329:1372	Topical application of hydrogel-based patches allowed the acceleration of wound healing and the complete healing, for composite hydrogel enriched with carotenoids.
32487393	0	30	theme	crab	13:16	arg1	hydrogel					45:52	A novel blue crab chitosan/protein composite hydrogel	0:52	A novel blue crab chitosan/protein composite hydrogel	0:52	A novel blue crab chitosan/protein composite hydrogel enriched with carotenoids endowed with distinguished wound healing capability: In vitro characterization and in vivo assessment.
32487393	0	31	theme	In	133:134	arg1	characterization					142:157	In vitro characterization	133:157	In vitro characterization	133:157	A novel blue crab chitosan/protein composite hydrogel enriched with carotenoids endowed with distinguished wound healing capability: In vitro characterization and in vivo assessment.
32487393	0	31	theme	In	133:134	arg1	carotenoids					68:78	carotenoids	68:78	carotenoids endowed with distinguished wound healing capability: In vitro characterization and in vivo assessment	68:180	A novel blue crab chitosan/protein composite hydrogel enriched with carotenoids endowed with distinguished wound healing capability: In vitro characterization and in vivo assessment.
32487393	0	32	dep	carotenoids	68:78	arg1	endowed					80:86	endowed	80:86	endowed	80:86	A novel blue crab chitosan/protein composite hydrogel enriched with carotenoids endowed with distinguished wound healing capability: In vitro characterization and in vivo assessment.
32487393	0	32	dep	carotenoids	68:78	arg1	characterization					142:157	In vitro characterization	133:157	In vitro characterization	133:157	A novel blue crab chitosan/protein composite hydrogel enriched with carotenoids endowed with distinguished wound healing capability: In vitro characterization and in vivo assessment.
32487393	0	32	dep	carotenoids	68:78	arg1	carotenoids					68:78	carotenoids	68:78	carotenoids endowed with distinguished wound healing capability: In vitro characterization and in vivo assessment	68:180	A novel blue crab chitosan/protein composite hydrogel enriched with carotenoids endowed with distinguished wound healing capability: In vitro characterization and in vivo assessment.
32487393	0	32	dep	carotenoids	68:78	arg1	assessment					171:180	in vivo assessment	163:180	in vivo assessment	163:180	A novel blue crab chitosan/protein composite hydrogel enriched with carotenoids endowed with distinguished wound healing capability: In vitro characterization and in vivo assessment.
32487393	2	33	theme	isolate	432:438	arg1	concentration					440:452	a protein isolate concentration	422:452	a protein isolate concentration not exceeding 15% (w/w)	422:476	By increasing the concentration of the protein isolate, chitosan hydrogels were more elastic at a protein isolate concentration not exceeding 15% (w/w).
32487393	0	34	theme	blue	8:11	arg1	crab					13:16	A novel blue crab	0:16	A novel blue crab chitosan/protein composite hydrogel	0:52	A novel blue crab chitosan/protein composite hydrogel enriched with carotenoids endowed with distinguished wound healing capability: In vitro characterization and in vivo assessment.
32487393	4	35	theme	protein	727:733	arg1	isolate					735:741	protein isolate	727:741	protein isolate	727:741	Thanks to its appropriate structural, swelling and mechanical resistance properties, chitosan hydrogel (3%; w/v), reinforced with 15% (w/w) of protein isolate, was selected for the carotenoids in vitro release study.
32487393	2	36	theme	protein	424:430	arg1	concentration					440:452	a protein isolate concentration	422:452	a protein isolate concentration not exceeding 15% (w/w)	422:476	By increasing the concentration of the protein isolate, chitosan hydrogels were more elastic at a protein isolate concentration not exceeding 15% (w/w).
32487393	0	37	theme	composite	35:43	arg1	hydrogel					45:52	A novel blue crab chitosan/protein composite hydrogel	0:52	A novel blue crab chitosan/protein composite hydrogel	0:52	A novel blue crab chitosan/protein composite hydrogel enriched with carotenoids endowed with distinguished wound healing capability: In vitro characterization and in vivo assessment.
32487393	1	38	theme	wound	311:315	arg1	healing					317:323	wound healing	311:323	wound healing	311:323	This work aimed to the development of chitosan and protein isolate composite hydrogels, for carotenoids-controlled delivery and wound healing.
32487393	6	39	theme	developed	986:994	arg1	hydrogels					996:1004	developed hydrogels	986:1004	developed hydrogels	986:1004	Thus, developed hydrogels could be applied as pH-sensitive intelligent carriers, for drugs-controlled release, with interesting antioxidant abilities.
32487393	6	39	theme	developed	986:994	arg1	carriers					1051:1058	pH-sensitive intelligent carriers	1026:1058	pH-sensitive intelligent carriers	1026:1058	Thus, developed hydrogels could be applied as pH-sensitive intelligent carriers, for drugs-controlled release, with interesting antioxidant abilities.
32487393	8	40	theme	healing	1291:1297	arg1	acceleration					1269:1280	the acceleration	1265:1280	the acceleration of wound healing and the complete healing	1265:1322	Topical application of hydrogel-based patches allowed the acceleration of wound healing and the complete healing, for composite hydrogel enriched with carotenoids.
32487393	5	41	dep	microenvironments	941:957	arg1	pH 4.0					960:965	pH 4.0	960:965	pH 4.0	960:965	Release profiles, show delivery patterns, where carotenoids were more barely released at a pH 7.4 medium (p < .05), compared to more acidic microenvironments (pH 4.0 and pH 2.0).
32487393	5	41	dep	microenvironments	941:957	arg1	microenvironments					941:957	more acidic microenvironments	929:957	more acidic microenvironments (pH 4.0 and pH 2.0)	929:977	Release profiles, show delivery patterns, where carotenoids were more barely released at a pH 7.4 medium (p < .05), compared to more acidic microenvironments (pH 4.0 and pH 2.0).
32487393	5	41	dep	microenvironments	941:957	arg1	pH 2.0					971:976	pH 2.0	971:976	pH 2.0	971:976	Release profiles, show delivery patterns, where carotenoids were more barely released at a pH 7.4 medium (p < .05), compared to more acidic microenvironments (pH 4.0 and pH 2.0).
32487393	0	42	theme	chitosan/protein	18:33	arg1	hydrogel					45:52	A novel blue crab chitosan/protein composite hydrogel	0:52	A novel blue crab chitosan/protein composite hydrogel	0:52	A novel blue crab chitosan/protein composite hydrogel enriched with carotenoids endowed with distinguished wound healing capability: In vitro characterization and in vivo assessment.
32487393	4	43	theme	appropriate	598:608	arg1	properties					657:666	its appropriate structural, swelling and mechanical resistance properties	594:666	its appropriate structural, swelling and mechanical resistance properties	594:666	Thanks to its appropriate structural, swelling and mechanical resistance properties, chitosan hydrogel (3%; w/v), reinforced with 15% (w/w) of protein isolate, was selected for the carotenoids in vitro release study.
32487393	8	44	theme	patches	1249:1255	arg1	application					1219:1229	Topical application	1211:1229	Topical application of hydrogel-based patches	1211:1255	Topical application of hydrogel-based patches allowed the acceleration of wound healing and the complete healing, for composite hydrogel enriched with carotenoids.
32487393	4	45	theme	release	786:792	arg1	study					794:798	the carotenoids in vitro release study	761:798	the carotenoids in vitro release study	761:798	Thanks to its appropriate structural, swelling and mechanical resistance properties, chitosan hydrogel (3%; w/v), reinforced with 15% (w/w) of protein isolate, was selected for the carotenoids in vitro release study.
32487393	5	46	theme	delivery	824:831	arg1	patterns					833:840	delivery patterns	824:840	delivery patterns	824:840	Release profiles, show delivery patterns, where carotenoids were more barely released at a pH 7.4 medium (p < .05), compared to more acidic microenvironments (pH 4.0 and pH 2.0).
32487393	7	47	theme	hydrogels	1164:1172	arg1	potential					1151:1159	The in vivo healing potential	1131:1159	The in vivo healing potential of hydrogels in rats' models	1131:1188	The in vivo healing potential of hydrogels in rats' models was further studied.
32487393	0	48	theme	in	163:164	arg1	carotenoids					68:78	carotenoids	68:78	carotenoids endowed with distinguished wound healing capability: In vitro characterization and in vivo assessment	68:180	A novel blue crab chitosan/protein composite hydrogel enriched with carotenoids endowed with distinguished wound healing capability: In vitro characterization and in vivo assessment.
32487393	0	48	theme	in	163:164	arg1	assessment					171:180	in vivo assessment	163:180	in vivo assessment	163:180	A novel blue crab chitosan/protein composite hydrogel enriched with carotenoids endowed with distinguished wound healing capability: In vitro characterization and in vivo assessment.
32487393	3	49	theme	isolate	496:502	arg1	hydrogels					514:522	Chitosan-protein isolate composite hydrogels	479:522	Chitosan-protein isolate composite hydrogels	479:522	Chitosan-protein isolate composite hydrogels revealed low cytotoxicity towards MG-63 osteosarcoma cells.
32487393	6	50	theme	antioxidant	1108:1118	arg1	abilities					1120:1128	interesting antioxidant abilities	1096:1128	interesting antioxidant abilities	1096:1128	Thus, developed hydrogels could be applied as pH-sensitive intelligent carriers, for drugs-controlled release, with interesting antioxidant abilities.
32487393	3	51	theme	composite	504:512	arg1	hydrogels					514:522	Chitosan-protein isolate composite hydrogels	479:522	Chitosan-protein isolate composite hydrogels	479:522	Chitosan-protein isolate composite hydrogels revealed low cytotoxicity towards MG-63 osteosarcoma cells.
32487393	4	52	theme	in	777:778	arg1	study					794:798	the carotenoids in vitro release study	761:798	the carotenoids in vitro release study	761:798	Thanks to its appropriate structural, swelling and mechanical resistance properties, chitosan hydrogel (3%; w/v), reinforced with 15% (w/w) of protein isolate, was selected for the carotenoids in vitro release study.
32487393	4	53	theme	carotenoids	765:775	arg1	study					794:798	the carotenoids in vitro release study	761:798	the carotenoids in vitro release study	761:798	Thanks to its appropriate structural, swelling and mechanical resistance properties, chitosan hydrogel (3%; w/v), reinforced with 15% (w/w) of protein isolate, was selected for the carotenoids in vitro release study.
32487393	4	54	dep	hydrogel	678:685	arg1	%					689:689	3%	688:689	3%; w/v	688:694	Thanks to its appropriate structural, swelling and mechanical resistance properties, chitosan hydrogel (3%; w/v), reinforced with 15% (w/w) of protein isolate, was selected for the carotenoids in vitro release study.
32487393	0	55	dep	in	163:164	arg1	vivo					166:169	vivo	166:169	vivo	166:169	A novel blue crab chitosan/protein composite hydrogel enriched with carotenoids endowed with distinguished wound healing capability: In vitro characterization and in vivo assessment.
32487393	4	56	theme	isolate	735:741	arg1	%					716:716	15%	714:716	15% (w/w) of protein isolate	714:741	Thanks to its appropriate structural, swelling and mechanical resistance properties, chitosan hydrogel (3%; w/v), reinforced with 15% (w/w) of protein isolate, was selected for the carotenoids in vitro release study.
32487393	4	56	theme	isolate	735:741	arg1	isolate					735:741	protein isolate	727:741	protein isolate	727:741	Thanks to its appropriate structural, swelling and mechanical resistance properties, chitosan hydrogel (3%; w/v), reinforced with 15% (w/w) of protein isolate, was selected for the carotenoids in vitro release study.
32487393	4	56	theme	isolate	735:741	arg1	w/w					719:721	w/w	719:721	w/w	719:721	Thanks to its appropriate structural, swelling and mechanical resistance properties, chitosan hydrogel (3%; w/v), reinforced with 15% (w/w) of protein isolate, was selected for the carotenoids in vitro release study.
32487393	1	57	theme	chitosan	221:228	arg1	hydrogels					260:268	chitosan and protein isolate composite hydrogels	221:268	chitosan and protein isolate composite hydrogels	221:268	This work aimed to the development of chitosan and protein isolate composite hydrogels, for carotenoids-controlled delivery and wound healing.
32487393	5	58	theme	acidic	934:939	arg1	pH 4.0					960:965	pH 4.0	960:965	pH 4.0	960:965	Release profiles, show delivery patterns, where carotenoids were more barely released at a pH 7.4 medium (p < .05), compared to more acidic microenvironments (pH 4.0 and pH 2.0).
32487393	5	58	theme	acidic	934:939	arg1	microenvironments					941:957	more acidic microenvironments	929:957	more acidic microenvironments (pH 4.0 and pH 2.0)	929:977	Release profiles, show delivery patterns, where carotenoids were more barely released at a pH 7.4 medium (p < .05), compared to more acidic microenvironments (pH 4.0 and pH 2.0).
32487393	5	58	theme	acidic	934:939	arg1	pH 2.0					971:976	pH 2.0	971:976	pH 2.0	971:976	Release profiles, show delivery patterns, where carotenoids were more barely released at a pH 7.4 medium (p < .05), compared to more acidic microenvironments (pH 4.0 and pH 2.0).
32487393	5	59	theme	Release	801:807	arg1	profiles					809:816	Release profiles,	801:817	profiles	809:816	Release profiles, show delivery patterns, where carotenoids were more barely released at a pH 7.4 medium (p < .05), compared to more acidic microenvironments (pH 4.0 and pH 2.0).
32487393	3	60	theme	Chitosan-protein	479:494	arg1	hydrogels					514:522	Chitosan-protein isolate composite hydrogels	479:522	Chitosan-protein isolate composite hydrogels	479:522	Chitosan-protein isolate composite hydrogels revealed low cytotoxicity towards MG-63 osteosarcoma cells.
32487393	5	61	theme	pH 7.4	892:897	arg1	p < .05					907:913	p < .05	907:913	p < .05	907:913	Release profiles, show delivery patterns, where carotenoids were more barely released at a pH 7.4 medium (p < .05), compared to more acidic microenvironments (pH 4.0 and pH 2.0).
32487393	5	61	theme	pH 7.4	892:897	arg1	medium					899:904	a pH 7.4 medium	890:904	a pH 7.4 medium (p < .05)	890:914	Release profiles, show delivery patterns, where carotenoids were more barely released at a pH 7.4 medium (p < .05), compared to more acidic microenvironments (pH 4.0 and pH 2.0).
32487393	8	62	theme	hydrogel-based	1234:1247	arg1	patches					1249:1255	hydrogel-based patches	1234:1255	hydrogel-based patches	1234:1255	Topical application of hydrogel-based patches allowed the acceleration of wound healing and the complete healing, for composite hydrogel enriched with carotenoids.
32487393	4	63	dep	%	689:689	arg1	w/v					692:694	w/v	692:694	3%; w/v	688:694	Thanks to its appropriate structural, swelling and mechanical resistance properties, chitosan hydrogel (3%; w/v), reinforced with 15% (w/w) of protein isolate, was selected for the carotenoids in vitro release study.
32487393	4	64	theme	chitosan	669:676	arg1	hydrogel					678:685	chitosan hydrogel	669:685	chitosan hydrogel (3%; w/v)	669:695	Thanks to its appropriate structural, swelling and mechanical resistance properties, chitosan hydrogel (3%; w/v), reinforced with 15% (w/w) of protein isolate, was selected for the carotenoids in vitro release study.
32487393	2	65	theme	chitosan	382:389	arg1	hydrogels					391:399	chitosan hydrogels	382:399	chitosan hydrogels	382:399	By increasing the concentration of the protein isolate, chitosan hydrogels were more elastic at a protein isolate concentration not exceeding 15% (w/w).
32487393	1	66	theme	protein	234:240	arg1	hydrogels					260:268	chitosan and protein isolate composite hydrogels	221:268	chitosan and protein isolate composite hydrogels	221:268	This work aimed to the development of chitosan and protein isolate composite hydrogels, for carotenoids-controlled delivery and wound healing.
32487393	4	67	theme	resistance	646:655	arg1	properties					657:666	its appropriate structural, swelling and mechanical resistance properties	594:666	its appropriate structural, swelling and mechanical resistance properties	594:666	Thanks to its appropriate structural, swelling and mechanical resistance properties, chitosan hydrogel (3%; w/v), reinforced with 15% (w/w) of protein isolate, was selected for the carotenoids in vitro release study.
32487393	4	68	theme	mechanical	635:644	arg1	properties					657:666	its appropriate structural, swelling and mechanical resistance properties	594:666	its appropriate structural, swelling and mechanical resistance properties	594:666	Thanks to its appropriate structural, swelling and mechanical resistance properties, chitosan hydrogel (3%; w/v), reinforced with 15% (w/w) of protein isolate, was selected for the carotenoids in vitro release study.
32487393	1	69	theme	isolate	242:248	arg1	hydrogels					260:268	chitosan and protein isolate composite hydrogels	221:268	chitosan and protein isolate composite hydrogels	221:268	This work aimed to the development of chitosan and protein isolate composite hydrogels, for carotenoids-controlled delivery and wound healing.
32219464	7	0	from	dioxide	1332:1338	arg1	product					1300:1306	the end product	1292:1306	the end product from methane and carbon dioxide	1292:1338	The sulfate addition had a significant impact on the carbon flow within the system, shifting the end product from methane and carbon dioxide to acetate and carbon dioxide.
32219464	2	1	theme	reduction	344:352	arg1	toxic					376:380	toxic	376:380	toxic	376:380	In addition, the end product of sulfate reduction, hydrogen sulfide, is toxic and corrosive.
32219464	2	1	theme	reduction	344:352	arg1	product					325:331	the end product	317:331	the end product of sulfate reduction	317:352	In addition, the end product of sulfate reduction, hydrogen sulfide, is toxic and corrosive.
32219464	2	1	theme	reduction	344:352	arg1	sulfide					364:370	hydrogen sulfide	355:370	hydrogen sulfide	355:370	In addition, the end product of sulfate reduction, hydrogen sulfide, is toxic and corrosive.
32219464	3	2	theme	microbial	538:546	arg1	community					548:556	the microbial community	534:556	the microbial community with quantitative PCR and amplicon sequencing of the 16s rRNA gene	534:623	Here we show how sulfate addition affects physiological processes in a thermophilic methanogenic system by analyzing the carbon flow and the microbial community with quantitative PCR and amplicon sequencing of the 16s rRNA gene.
32219464	9	3	theme	sulfate	1683:1689	arg1	introduction					1667:1678	unexpected introduction	1656:1678	unexpected introduction of sulfate	1656:1689	Despite the strong impact of sulfate addition on the carbon flow and the microbial community structure during thermophilic biogas production, short-term process disturbances caused by unexpected introduction of sulfate may be overcome due to the high resilience of the engaged microorganisms.
32219464	4	4	theme	0.5	648:650	arg1	addition					636:643	A sulfate addition	626:643	A sulfate addition of 0.5 to 3 g/L	626:659	A sulfate addition of 0.5 to 3 g/L caused a decline in methane production by 73-92%, while higher sulfate concentrations had no additional inhibitory effect.
32219464	1	5	theme	biogas	176:181	arg1	production					183:192	biogas production	176:192	biogas production	176:192	Substrates with high sulfate levels pose problems for biogas production as they allow sulfate reducing bacteria to compete with syntrophic and methanogenic members of the community.
32219464	9	6	theme	community	1555:1563	arg1	structure					1565:1573	the microbial community structure	1541:1573	the microbial community structure	1541:1573	Despite the strong impact of sulfate addition on the carbon flow and the microbial community structure during thermophilic biogas production, short-term process disturbances caused by unexpected introduction of sulfate may be overcome due to the high resilience of the engaged microorganisms.
32219464	6	7	theme	reducing	1075:1082	arg1	Desulforudis					1116:1127	Desulforudis	1116:1127	Desulforudis	1116:1127	The abundance of methanogens (e.g., Methanoculleus and Methanosarcina) was reduced, while sulfate reducing bacteria (especially Candidatus Desulforudis and Desulfotomaculum) increased significantly in presence of sulfate.
32219464	6	7	theme	reducing	1075:1082	arg1	bacteria					1084:1091	sulfate reducing bacteria	1067:1091	sulfate reducing bacteria (especially Candidatus Desulforudis and Desulfotomaculum)	1067:1149	The abundance of methanogens (e.g., Methanoculleus and Methanosarcina) was reduced, while sulfate reducing bacteria (especially Candidatus Desulforudis and Desulfotomaculum) increased significantly in presence of sulfate.
32219464	6	7	theme	reducing	1075:1082	arg1	Desulfotomaculum					1133:1148	Desulfotomaculum	1133:1148	Desulfotomaculum	1133:1148	The abundance of methanogens (e.g., Methanoculleus and Methanosarcina) was reduced, while sulfate reducing bacteria (especially Candidatus Desulforudis and Desulfotomaculum) increased significantly in presence of sulfate.
32219464	4	8	contain	had	747:749	arg1	concentrations					732:745	higher sulfate concentrations	717:745	higher sulfate concentrations	717:745	A sulfate addition of 0.5 to 3 g/L caused a decline in methane production by 73-92%, while higher sulfate concentrations had no additional inhibitory effect.
32219464	4	8	contain	had	747:749	arg2	effect					776:781	no additional inhibitory effect	751:781	no additional inhibitory effect	751:781	A sulfate addition of 0.5 to 3 g/L caused a decline in methane production by 73-92%, while higher sulfate concentrations had no additional inhibitory effect.
32219464	5	9	theme	sulfate	795:801	arg1	addition					803:810	sulfate addition	795:810	sulfate addition	795:810	Generally, sulfate addition induced a shift in the composition of the microbial community towards a higher dominance of Firmicutes and decreasing abundances of Bacteroidetes and Euryarchaeota.
32219464	5	10	from	shift	822:826	arg1	composition					835:845	the composition	831:845	the composition of the microbial community	831:872	Generally, sulfate addition induced a shift in the composition of the microbial community towards a higher dominance of Firmicutes and decreasing abundances of Bacteroidetes and Euryarchaeota.
32219464	1	11	theme	reducing	216:223	arg1	bacteria					225:232	sulfate reducing bacteria	208:232	sulfate reducing bacteria	208:232	Substrates with high sulfate levels pose problems for biogas production as they allow sulfate reducing bacteria to compete with syntrophic and methanogenic members of the community.
32219464	3	12	theme	amplicon	584:591	arg1	sequencing					593:602	amplicon sequencing	584:602	amplicon sequencing	584:602	Here we show how sulfate addition affects physiological processes in a thermophilic methanogenic system by analyzing the carbon flow and the microbial community with quantitative PCR and amplicon sequencing of the 16s rRNA gene.
32219464	9	13	theme	strong	1484:1489	arg1	impact					1491:1496	the strong impact	1480:1496	the strong impact of sulfate addition on the carbon flow and the microbial community structure during thermophilic biogas production	1480:1611	Despite the strong impact of sulfate addition on the carbon flow and the microbial community structure during thermophilic biogas production, short-term process disturbances caused by unexpected introduction of sulfate may be overcome due to the high resilience of the engaged microorganisms.
32219464	3	14	theme	gene	620:623	arg1	sequencing					593:602	amplicon sequencing	584:602	amplicon sequencing	584:602	Here we show how sulfate addition affects physiological processes in a thermophilic methanogenic system by analyzing the carbon flow and the microbial community with quantitative PCR and amplicon sequencing of the 16s rRNA gene.
32219464	3	14	theme	gene	620:623	arg1	PCR					576:578	quantitative PCR	563:578	quantitative PCR	563:578	Here we show how sulfate addition affects physiological processes in a thermophilic methanogenic system by analyzing the carbon flow and the microbial community with quantitative PCR and amplicon sequencing of the 16s rRNA gene.
32219464	1	15	theme	high	138:141	arg1	levels					151:156	high sulfate levels	138:156	high sulfate levels	138:156	Substrates with high sulfate levels pose problems for biogas production as they allow sulfate reducing bacteria to compete with syntrophic and methanogenic members of the community.
32219464	8	16	located	present	1449:1455	arg1	system					1464:1469	the system	1460:1469	the system	1460:1469	Interestingly, methane production quickly resumed, when sulfate was no longer present in the system.
32219464	8	16	located	present	1449:1455	arg2	sulfate					1427:1433	sulfate	1427:1433	sulfate	1427:1433	Interestingly, methane production quickly resumed, when sulfate was no longer present in the system.
32219464	9	17	theme	microorganisms	1749:1762	arg1	resilience					1723:1732	the high resilience	1714:1732	the high resilience of the engaged microorganisms	1714:1762	Despite the strong impact of sulfate addition on the carbon flow and the microbial community structure during thermophilic biogas production, short-term process disturbances caused by unexpected introduction of sulfate may be overcome due to the high resilience of the engaged microorganisms.
32219464	8	18	from	present	1449:1455	arg1	system					1464:1469	the system	1460:1469	the system	1460:1469	Interestingly, methane production quickly resumed, when sulfate was no longer present in the system.
32219464	3	19	theme	16s	611:613	arg1	gene					620:623	the 16s rRNA gene	607:623	the 16s rRNA gene	607:623	Here we show how sulfate addition affects physiological processes in a thermophilic methanogenic system by analyzing the carbon flow and the microbial community with quantitative PCR and amplicon sequencing of the 16s rRNA gene.
32219464	5	20	theme	community	864:872	arg1	composition					835:845	the composition	831:845	the composition of the microbial community	831:872	Generally, sulfate addition induced a shift in the composition of the microbial community towards a higher dominance of Firmicutes and decreasing abundances of Bacteroidetes and Euryarchaeota.
32219464	2	21	from	addition	307:314	arg1	toxic					376:380	toxic	376:380	toxic	376:380	In addition, the end product of sulfate reduction, hydrogen sulfide, is toxic and corrosive.
32219464	2	21	from	addition	307:314	arg1	product					325:331	the end product	317:331	the end product of sulfate reduction	317:352	In addition, the end product of sulfate reduction, hydrogen sulfide, is toxic and corrosive.
32219464	7	22	theme	significant	1226:1236	arg1	impact					1238:1243	a significant impact	1224:1243	a significant impact	1224:1243	The sulfate addition had a significant impact on the carbon flow within the system, shifting the end product from methane and carbon dioxide to acetate and carbon dioxide.
32219464	0	23	from	Effect	0:5	arg1	composition					66:76	microbial community composition	46:76	microbial community composition	46:76	Effect of sulfate addition on carbon flow and microbial community composition during thermophilic digestion of cellulose.
32219464	0	23	from	Effect	0:5	arg1	flow					37:40	carbon flow	30:40	carbon flow	30:40	Effect of sulfate addition on carbon flow and microbial community composition during thermophilic digestion of cellulose.
32219464	0	24	theme	thermophilic	85:96	arg1	digestion					98:106	thermophilic digestion	85:106	thermophilic digestion of cellulose	85:119	Effect of sulfate addition on carbon flow and microbial community composition during thermophilic digestion of cellulose.
32219464	5	25	theme	Firmicutes	904:913	arg1	abundances					930:939	decreasing abundances	919:939	decreasing abundances of Bacteroidetes and Euryarchaeota	919:974	Generally, sulfate addition induced a shift in the composition of the microbial community towards a higher dominance of Firmicutes and decreasing abundances of Bacteroidetes and Euryarchaeota.
32219464	5	25	theme	Firmicutes	904:913	arg1	dominance					891:899	a higher dominance	882:899	a higher dominance of Firmicutes	882:913	Generally, sulfate addition induced a shift in the composition of the microbial community towards a higher dominance of Firmicutes and decreasing abundances of Bacteroidetes and Euryarchaeota.
32219464	3	26	theme	sulfate	414:420	arg1	addition					422:429	sulfate addition	414:429	sulfate addition	414:429	Here we show how sulfate addition affects physiological processes in a thermophilic methanogenic system by analyzing the carbon flow and the microbial community with quantitative PCR and amplicon sequencing of the 16s rRNA gene.
32219464	7	27	theme	sulfate	1203:1209	arg1	addition					1211:1218	The sulfate addition	1199:1218	The sulfate addition	1199:1218	The sulfate addition had a significant impact on the carbon flow within the system, shifting the end product from methane and carbon dioxide to acetate and carbon dioxide.
32219464	0	28	theme	carbon	30:35	arg1	flow					37:40	carbon flow	30:40	carbon flow	30:40	Effect of sulfate addition on carbon flow and microbial community composition during thermophilic digestion of cellulose.
32219464	1	29	with	Substrates	122:131	arg1	levels					151:156	high sulfate levels	138:156	high sulfate levels	138:156	Substrates with high sulfate levels pose problems for biogas production as they allow sulfate reducing bacteria to compete with syntrophic and methanogenic members of the community.
32219464	5	30	theme	decreasing	919:928	arg1	abundances					930:939	decreasing abundances	919:939	decreasing abundances of Bacteroidetes and Euryarchaeota	919:974	Generally, sulfate addition induced a shift in the composition of the microbial community towards a higher dominance of Firmicutes and decreasing abundances of Bacteroidetes and Euryarchaeota.
32219464	4	31	theme	higher	717:722	arg1	concentrations					732:745	higher sulfate concentrations	717:745	higher sulfate concentrations	717:745	A sulfate addition of 0.5 to 3 g/L caused a decline in methane production by 73-92%, while higher sulfate concentrations had no additional inhibitory effect.
32219464	0	32	theme	sulfate	10:16	arg1	addition					18:25	sulfate addition	10:25	sulfate addition	10:25	Effect of sulfate addition on carbon flow and microbial community composition during thermophilic digestion of cellulose.
32219464	1	33	theme	community	293:301	arg1	members					278:284	syntrophic and methanogenic members	250:284	syntrophic and methanogenic members of the community	250:301	Substrates with high sulfate levels pose problems for biogas production as they allow sulfate reducing bacteria to compete with syntrophic and methanogenic members of the community.
32219464	9	34	theme	sulfate	1501:1507	arg1	addition					1509:1516	sulfate addition	1501:1516	sulfate addition	1501:1516	Despite the strong impact of sulfate addition on the carbon flow and the microbial community structure during thermophilic biogas production, short-term process disturbances caused by unexpected introduction of sulfate may be overcome due to the high resilience of the engaged microorganisms.
32219464	6	35	dep	bacteria	1084:1091	arg1	Desulforudis					1116:1127	Desulforudis	1116:1127	Desulforudis	1116:1127	The abundance of methanogens (e.g., Methanoculleus and Methanosarcina) was reduced, while sulfate reducing bacteria (especially Candidatus Desulforudis and Desulfotomaculum) increased significantly in presence of sulfate.
32219464	6	35	dep	bacteria	1084:1091	arg1	bacteria					1084:1091	sulfate reducing bacteria	1067:1091	sulfate reducing bacteria (especially Candidatus Desulforudis and Desulfotomaculum)	1067:1149	The abundance of methanogens (e.g., Methanoculleus and Methanosarcina) was reduced, while sulfate reducing bacteria (especially Candidatus Desulforudis and Desulfotomaculum) increased significantly in presence of sulfate.
32219464	6	35	dep	bacteria	1084:1091	arg1	Desulfotomaculum					1133:1148	Desulfotomaculum	1133:1148	Desulfotomaculum	1133:1148	The abundance of methanogens (e.g., Methanoculleus and Methanosarcina) was reduced, while sulfate reducing bacteria (especially Candidatus Desulforudis and Desulfotomaculum) increased significantly in presence of sulfate.
32219464	9	36	theme	biogas	1595:1600	arg1	production					1602:1611	thermophilic biogas production	1582:1611	thermophilic biogas production	1582:1611	Despite the strong impact of sulfate addition on the carbon flow and the microbial community structure during thermophilic biogas production, short-term process disturbances caused by unexpected introduction of sulfate may be overcome due to the high resilience of the engaged microorganisms.
32219464	7	37	theme	end	1296:1298	arg1	product					1300:1306	the end product	1292:1306	the end product from methane and carbon dioxide	1292:1338	The sulfate addition had a significant impact on the carbon flow within the system, shifting the end product from methane and carbon dioxide to acetate and carbon dioxide.
32219464	0	38	theme	microbial	46:54	arg1	composition					66:76	microbial community composition	46:76	microbial community composition	46:76	Effect of sulfate addition on carbon flow and microbial community composition during thermophilic digestion of cellulose.
32219464	3	39	theme	methanogenic	481:492	arg1	system					494:499	a thermophilic methanogenic system	466:499	a thermophilic methanogenic system	466:499	Here we show how sulfate addition affects physiological processes in a thermophilic methanogenic system by analyzing the carbon flow and the microbial community with quantitative PCR and amplicon sequencing of the 16s rRNA gene.
32219464	4	40	theme	inhibitory	765:774	arg1	effect					776:781	no additional inhibitory effect	751:781	no additional inhibitory effect	751:781	A sulfate addition of 0.5 to 3 g/L caused a decline in methane production by 73-92%, while higher sulfate concentrations had no additional inhibitory effect.
32219464	9	41	theme	process	1625:1631	arg1	disturbances					1633:1644	short-term process disturbances	1614:1644	short-term process disturbances caused by unexpected introduction of sulfate	1614:1689	Despite the strong impact of sulfate addition on the carbon flow and the microbial community structure during thermophilic biogas production, short-term process disturbances caused by unexpected introduction of sulfate may be overcome due to the high resilience of the engaged microorganisms.
32219464	7	42	from	methane	1313:1319	arg1	product					1300:1306	the end product	1292:1306	the end product from methane and carbon dioxide	1292:1338	The sulfate addition had a significant impact on the carbon flow within the system, shifting the end product from methane and carbon dioxide to acetate and carbon dioxide.
32219464	9	43	theme	addition	1509:1516	arg1	impact					1491:1496	the strong impact	1480:1496	the strong impact of sulfate addition on the carbon flow and the microbial community structure during thermophilic biogas production	1480:1611	Despite the strong impact of sulfate addition on the carbon flow and the microbial community structure during thermophilic biogas production, short-term process disturbances caused by unexpected introduction of sulfate may be overcome due to the high resilience of the engaged microorganisms.
32219464	4	44	from	decline	670:676	arg1	production					689:698	methane production	681:698	methane production	681:698	A sulfate addition of 0.5 to 3 g/L caused a decline in methane production by 73-92%, while higher sulfate concentrations had no additional inhibitory effect.
32219464	1	45	theme	syntrophic	250:259	arg1	members					278:284	syntrophic and methanogenic members	250:284	syntrophic and methanogenic members of the community	250:301	Substrates with high sulfate levels pose problems for biogas production as they allow sulfate reducing bacteria to compete with syntrophic and methanogenic members of the community.
32219464	9	46	theme	unexpected	1656:1665	arg1	introduction					1667:1678	unexpected introduction	1656:1678	unexpected introduction of sulfate	1656:1689	Despite the strong impact of sulfate addition on the carbon flow and the microbial community structure during thermophilic biogas production, short-term process disturbances caused by unexpected introduction of sulfate may be overcome due to the high resilience of the engaged microorganisms.
32219464	7	47	theme	carbon	1252:1257	arg1	flow					1259:1262	the carbon flow	1248:1262	the carbon flow within the system	1248:1280	The sulfate addition had a significant impact on the carbon flow within the system, shifting the end product from methane and carbon dioxide to acetate and carbon dioxide.
32219464	1	48	theme	methanogenic	265:276	arg1	members					278:284	syntrophic and methanogenic members	250:284	syntrophic and methanogenic members of the community	250:301	Substrates with high sulfate levels pose problems for biogas production as they allow sulfate reducing bacteria to compete with syntrophic and methanogenic members of the community.
32219464	6	49	theme	sulfate	1190:1196	arg1	presence					1178:1185	presence	1178:1185	presence of sulfate	1178:1196	The abundance of methanogens (e.g., Methanoculleus and Methanosarcina) was reduced, while sulfate reducing bacteria (especially Candidatus Desulforudis and Desulfotomaculum) increased significantly in presence of sulfate.
32219464	2	50	theme	sulfate	336:342	arg1	reduction					344:352	sulfate reduction	336:352	sulfate reduction	336:352	In addition, the end product of sulfate reduction, hydrogen sulfide, is toxic and corrosive.
32219464	9	51	from	impact	1491:1496	arg1	structure					1565:1573	the microbial community structure	1541:1573	the microbial community structure	1541:1573	Despite the strong impact of sulfate addition on the carbon flow and the microbial community structure during thermophilic biogas production, short-term process disturbances caused by unexpected introduction of sulfate may be overcome due to the high resilience of the engaged microorganisms.
32219464	9	51	from	impact	1491:1496	arg1	flow					1532:1535	the carbon flow	1521:1535	the carbon flow	1521:1535	Despite the strong impact of sulfate addition on the carbon flow and the microbial community structure during thermophilic biogas production, short-term process disturbances caused by unexpected introduction of sulfate may be overcome due to the high resilience of the engaged microorganisms.
32219464	3	52	theme	quantitative	563:574	arg1	PCR					576:578	quantitative PCR	563:578	quantitative PCR	563:578	Here we show how sulfate addition affects physiological processes in a thermophilic methanogenic system by analyzing the carbon flow and the microbial community with quantitative PCR and amplicon sequencing of the 16s rRNA gene.
32219464	3	53	from	processes	453:461	arg1	system					494:499	a thermophilic methanogenic system	466:499	a thermophilic methanogenic system	466:499	Here we show how sulfate addition affects physiological processes in a thermophilic methanogenic system by analyzing the carbon flow and the microbial community with quantitative PCR and amplicon sequencing of the 16s rRNA gene.
32219464	1	54	theme	sulfate	143:149	arg1	levels					151:156	high sulfate levels	138:156	high sulfate levels	138:156	Substrates with high sulfate levels pose problems for biogas production as they allow sulfate reducing bacteria to compete with syntrophic and methanogenic members of the community.
32219464	1	55	theme	sulfate	208:214	arg1	bacteria					225:232	sulfate reducing bacteria	208:232	sulfate reducing bacteria	208:232	Substrates with high sulfate levels pose problems for biogas production as they allow sulfate reducing bacteria to compete with syntrophic and methanogenic members of the community.
32219464	9	56	theme	high	1718:1721	arg1	resilience					1723:1732	the high resilience	1714:1732	the high resilience of the engaged microorganisms	1714:1762	Despite the strong impact of sulfate addition on the carbon flow and the microbial community structure during thermophilic biogas production, short-term process disturbances caused by unexpected introduction of sulfate may be overcome due to the high resilience of the engaged microorganisms.
32219464	4	57	theme	sulfate	628:634	arg1	addition					636:643	A sulfate addition	626:643	A sulfate addition of 0.5 to 3 g/L	626:659	A sulfate addition of 0.5 to 3 g/L caused a decline in methane production by 73-92%, while higher sulfate concentrations had no additional inhibitory effect.
32219464	5	58	theme	higher	884:889	arg1	dominance					891:899	a higher dominance	882:899	a higher dominance of Firmicutes	882:913	Generally, sulfate addition induced a shift in the composition of the microbial community towards a higher dominance of Firmicutes and decreasing abundances of Bacteroidetes and Euryarchaeota.
32219464	4	59	theme	methane	681:687	arg1	production					689:698	methane production	681:698	methane production	681:698	A sulfate addition of 0.5 to 3 g/L caused a decline in methane production by 73-92%, while higher sulfate concentrations had no additional inhibitory effect.
32219464	3	60	theme	rRNA	615:618	arg1	gene					620:623	the 16s rRNA gene	607:623	the 16s rRNA gene	607:623	Here we show how sulfate addition affects physiological processes in a thermophilic methanogenic system by analyzing the carbon flow and the microbial community with quantitative PCR and amplicon sequencing of the 16s rRNA gene.
32219464	9	61	theme	engaged	1741:1747	arg1	microorganisms					1749:1762	the engaged microorganisms	1737:1762	the engaged microorganisms	1737:1762	Despite the strong impact of sulfate addition on the carbon flow and the microbial community structure during thermophilic biogas production, short-term process disturbances caused by unexpected introduction of sulfate may be overcome due to the high resilience of the engaged microorganisms.
32219464	7	62	theme	carbon	1355:1360	arg1	dioxide					1362:1368	carbon dioxide	1355:1368	carbon dioxide	1355:1368	The sulfate addition had a significant impact on the carbon flow within the system, shifting the end product from methane and carbon dioxide to acetate and carbon dioxide.
32219464	0	63	theme	community	56:64	arg1	composition					66:76	microbial community composition	46:76	microbial community composition	46:76	Effect of sulfate addition on carbon flow and microbial community composition during thermophilic digestion of cellulose.
32219464	5	64	theme	microbial	854:862	arg1	community					864:872	the microbial community	850:872	the microbial community	850:872	Generally, sulfate addition induced a shift in the composition of the microbial community towards a higher dominance of Firmicutes and decreasing abundances of Bacteroidetes and Euryarchaeota.
32219464	6	65	theme	methanogens	994:1004	arg1	abundance					981:989	The abundance	977:989	The abundance of methanogens (e.g., Methanoculleus and Methanosarcina)	977:1046	The abundance of methanogens (e.g., Methanoculleus and Methanosarcina) was reduced, while sulfate reducing bacteria (especially Candidatus Desulforudis and Desulfotomaculum) increased significantly in presence of sulfate.
32219464	5	66	theme	Bacteroidetes	944:956	arg1	abundances					930:939	decreasing abundances	919:939	decreasing abundances of Bacteroidetes and Euryarchaeota	919:974	Generally, sulfate addition induced a shift in the composition of the microbial community towards a higher dominance of Firmicutes and decreasing abundances of Bacteroidetes and Euryarchaeota.
32219464	5	66	theme	Bacteroidetes	944:956	arg1	dominance					891:899	a higher dominance	882:899	a higher dominance of Firmicutes	882:913	Generally, sulfate addition induced a shift in the composition of the microbial community towards a higher dominance of Firmicutes and decreasing abundances of Bacteroidetes and Euryarchaeota.
32219464	4	67	theme	sulfate	724:730	arg1	concentrations					732:745	higher sulfate concentrations	717:745	higher sulfate concentrations	717:745	A sulfate addition of 0.5 to 3 g/L caused a decline in methane production by 73-92%, while higher sulfate concentrations had no additional inhibitory effect.
32219464	3	68	theme	thermophilic	468:479	arg1	system					494:499	a thermophilic methanogenic system	466:499	a thermophilic methanogenic system	466:499	Here we show how sulfate addition affects physiological processes in a thermophilic methanogenic system by analyzing the carbon flow and the microbial community with quantitative PCR and amplicon sequencing of the 16s rRNA gene.
32219464	8	69	from	system	1464:1469	arg1	present					1449:1455	present	1449:1455	present	1449:1455	Interestingly, methane production quickly resumed, when sulfate was no longer present in the system.
32219464	5	70	theme	Euryarchaeota	962:974	arg1	abundances					930:939	decreasing abundances	919:939	decreasing abundances of Bacteroidetes and Euryarchaeota	919:974	Generally, sulfate addition induced a shift in the composition of the microbial community towards a higher dominance of Firmicutes and decreasing abundances of Bacteroidetes and Euryarchaeota.
32219464	5	70	theme	Euryarchaeota	962:974	arg1	dominance					891:899	a higher dominance	882:899	a higher dominance of Firmicutes	882:913	Generally, sulfate addition induced a shift in the composition of the microbial community towards a higher dominance of Firmicutes and decreasing abundances of Bacteroidetes and Euryarchaeota.
32219464	6	71	theme	sulfate	1067:1073	arg1	Desulforudis					1116:1127	Desulforudis	1116:1127	Desulforudis	1116:1127	The abundance of methanogens (e.g., Methanoculleus and Methanosarcina) was reduced, while sulfate reducing bacteria (especially Candidatus Desulforudis and Desulfotomaculum) increased significantly in presence of sulfate.
32219464	6	71	theme	sulfate	1067:1073	arg1	bacteria					1084:1091	sulfate reducing bacteria	1067:1091	sulfate reducing bacteria (especially Candidatus Desulforudis and Desulfotomaculum)	1067:1149	The abundance of methanogens (e.g., Methanoculleus and Methanosarcina) was reduced, while sulfate reducing bacteria (especially Candidatus Desulforudis and Desulfotomaculum) increased significantly in presence of sulfate.
32219464	6	71	theme	sulfate	1067:1073	arg1	Desulfotomaculum					1133:1148	Desulfotomaculum	1133:1148	Desulfotomaculum	1133:1148	The abundance of methanogens (e.g., Methanoculleus and Methanosarcina) was reduced, while sulfate reducing bacteria (especially Candidatus Desulforudis and Desulfotomaculum) increased significantly in presence of sulfate.
32219464	6	72	dep	Methanoculleus	1013:1026	arg1	e.g.					1007:1010	e.g.	1007:1010	e.g.	1007:1010	The abundance of methanogens (e.g., Methanoculleus and Methanosarcina) was reduced, while sulfate reducing bacteria (especially Candidatus Desulforudis and Desulfotomaculum) increased significantly in presence of sulfate.
32219464	0	73	theme	cellulose	111:119	arg1	digestion					98:106	thermophilic digestion	85:106	thermophilic digestion of cellulose	85:119	Effect of sulfate addition on carbon flow and microbial community composition during thermophilic digestion of cellulose.
32219464	3	74	with	community	548:556	arg1	sequencing					593:602	amplicon sequencing	584:602	amplicon sequencing	584:602	Here we show how sulfate addition affects physiological processes in a thermophilic methanogenic system by analyzing the carbon flow and the microbial community with quantitative PCR and amplicon sequencing of the 16s rRNA gene.
32219464	3	74	with	community	548:556	arg1	PCR					576:578	quantitative PCR	563:578	quantitative PCR	563:578	Here we show how sulfate addition affects physiological processes in a thermophilic methanogenic system by analyzing the carbon flow and the microbial community with quantitative PCR and amplicon sequencing of the 16s rRNA gene.
32219464	9	75	theme	microbial	1545:1553	arg1	structure					1565:1573	the microbial community structure	1541:1573	the microbial community structure	1541:1573	Despite the strong impact of sulfate addition on the carbon flow and the microbial community structure during thermophilic biogas production, short-term process disturbances caused by unexpected introduction of sulfate may be overcome due to the high resilience of the engaged microorganisms.
32219464	0	76	theme	addition	18:25	arg1	Effect					0:5	Effect	0:5	Effect of sulfate addition on carbon flow and microbial community composition during thermophilic digestion of cellulose.	0:120	Effect of sulfate addition on carbon flow and microbial community composition during thermophilic digestion of cellulose.
32219464	3	77	with	flow	525:528	arg1	sequencing					593:602	amplicon sequencing	584:602	amplicon sequencing	584:602	Here we show how sulfate addition affects physiological processes in a thermophilic methanogenic system by analyzing the carbon flow and the microbial community with quantitative PCR and amplicon sequencing of the 16s rRNA gene.
32219464	3	77	with	flow	525:528	arg1	PCR					576:578	quantitative PCR	563:578	quantitative PCR	563:578	Here we show how sulfate addition affects physiological processes in a thermophilic methanogenic system by analyzing the carbon flow and the microbial community with quantitative PCR and amplicon sequencing of the 16s rRNA gene.
32219464	9	78	theme	thermophilic	1582:1593	arg1	production					1602:1611	thermophilic biogas production	1582:1611	thermophilic biogas production	1582:1611	Despite the strong impact of sulfate addition on the carbon flow and the microbial community structure during thermophilic biogas production, short-term process disturbances caused by unexpected introduction of sulfate may be overcome due to the high resilience of the engaged microorganisms.
32219464	7	79	theme	carbon	1325:1330	arg1	dioxide					1332:1338	carbon dioxide	1325:1338	carbon dioxide	1325:1338	The sulfate addition had a significant impact on the carbon flow within the system, shifting the end product from methane and carbon dioxide to acetate and carbon dioxide.
32219464	8	80	theme	methane	1386:1392	arg1	production					1394:1403	methane production	1386:1403	methane production	1386:1403	Interestingly, methane production quickly resumed, when sulfate was no longer present in the system.
32219464	3	81	theme	physiological	439:451	arg1	processes					453:461	physiological processes	439:461	physiological processes in a thermophilic methanogenic system	439:499	Here we show how sulfate addition affects physiological processes in a thermophilic methanogenic system by analyzing the carbon flow and the microbial community with quantitative PCR and amplicon sequencing of the 16s rRNA gene.
32219464	2	82	theme	hydrogen	355:362	arg1	product					325:331	the end product	317:331	the end product of sulfate reduction	317:352	In addition, the end product of sulfate reduction, hydrogen sulfide, is toxic and corrosive.
32219464	2	82	theme	hydrogen	355:362	arg1	sulfide					364:370	hydrogen sulfide	355:370	hydrogen sulfide	355:370	In addition, the end product of sulfate reduction, hydrogen sulfide, is toxic and corrosive.
32219464	9	83	theme	carbon	1525:1530	arg1	flow					1532:1535	the carbon flow	1521:1535	the carbon flow	1521:1535	Despite the strong impact of sulfate addition on the carbon flow and the microbial community structure during thermophilic biogas production, short-term process disturbances caused by unexpected introduction of sulfate may be overcome due to the high resilience of the engaged microorganisms.
32219464	7	84	contain	had	1220:1222	arg1	addition					1211:1218	The sulfate addition	1199:1218	The sulfate addition	1199:1218	The sulfate addition had a significant impact on the carbon flow within the system, shifting the end product from methane and carbon dioxide to acetate and carbon dioxide.
32219464	7	84	contain	had	1220:1222	arg2	impact					1238:1243	a significant impact	1224:1243	a significant impact	1224:1243	The sulfate addition had a significant impact on the carbon flow within the system, shifting the end product from methane and carbon dioxide to acetate and carbon dioxide.
32219464	9	85	theme	short-term	1614:1623	arg1	disturbances					1633:1644	short-term process disturbances	1614:1644	short-term process disturbances caused by unexpected introduction of sulfate	1614:1689	Despite the strong impact of sulfate addition on the carbon flow and the microbial community structure during thermophilic biogas production, short-term process disturbances caused by unexpected introduction of sulfate may be overcome due to the high resilience of the engaged microorganisms.
32219464	2	86	from	toxic	376:380	arg1	addition					307:314	addition	307:314	addition	307:314	In addition, the end product of sulfate reduction, hydrogen sulfide, is toxic and corrosive.
32219464	4	87	theme	additional	754:763	arg1	effect					776:781	no additional inhibitory effect	751:781	no additional inhibitory effect	751:781	A sulfate addition of 0.5 to 3 g/L caused a decline in methane production by 73-92%, while higher sulfate concentrations had no additional inhibitory effect.
32219464	2	88	theme	end	321:323	arg1	toxic					376:380	toxic	376:380	toxic	376:380	In addition, the end product of sulfate reduction, hydrogen sulfide, is toxic and corrosive.
32219464	2	88	theme	end	321:323	arg1	product					325:331	the end product	317:331	the end product of sulfate reduction	317:352	In addition, the end product of sulfate reduction, hydrogen sulfide, is toxic and corrosive.
32219464	2	88	theme	end	321:323	arg1	sulfide					364:370	hydrogen sulfide	355:370	hydrogen sulfide	355:370	In addition, the end product of sulfate reduction, hydrogen sulfide, is toxic and corrosive.
32219464	8	89	attach	present	1449:1455	arg1	system					1464:1469	the system	1460:1469	the system	1460:1469	Interestingly, methane production quickly resumed, when sulfate was no longer present in the system.
32219464	8	89	attach	present	1449:1455	arg2	sulfate					1427:1433	sulfate	1427:1433	sulfate	1427:1433	Interestingly, methane production quickly resumed, when sulfate was no longer present in the system.
32219464	3	90	theme	carbon	518:523	arg1	flow					525:528	the carbon flow	514:528	the carbon flow	514:528	Here we show how sulfate addition affects physiological processes in a thermophilic methanogenic system by analyzing the carbon flow and the microbial community with quantitative PCR and amplicon sequencing of the 16s rRNA gene.
33419222	5	0	theme	liquid	764:769	arg1	HPLC					787:790	HPLC	787:790	HPLC	787:790	Based on analyses of changes in body chemical composition using Fourier transform infrared (FTIR) and changes in cryoprotectant content using high performance liquid chromatography (HPLC), we showed how aphid C. tujafilina adapted to overwintering as an active stage.
33419222	5	0	theme	liquid	764:769	arg1	chromatography					771:784	high performance liquid chromatography	747:784	high performance liquid chromatography (HPLC)	747:791	Based on analyses of changes in body chemical composition using Fourier transform infrared (FTIR) and changes in cryoprotectant content using high performance liquid chromatography (HPLC), we showed how aphid C. tujafilina adapted to overwintering as an active stage.
33419222	6	1	located	observed	1012:1019	arg1	spectrum					885:892	the FTIR spectrum	876:892	the FTIR spectrum of the winter type of C. tujafilina	876:928	In the FTIR spectrum of the winter type of C. tujafilina, higher peak values originating from the carbohydrates, proteins and lipids, were observed.
33419222	6	1	located	observed	1012:1019	arg2	values					943:948	higher peak values	931:948	higher peak values originating from the carbohydrates, proteins and lipids,	931:1005	In the FTIR spectrum of the winter type of C. tujafilina, higher peak values originating from the carbohydrates, proteins and lipids, were observed.
33419222	5	2	theme	body	637:640	arg1	composition					651:661	body chemical composition	637:661	body chemical composition using Fourier transform infrared (FTIR)	637:701	Based on analyses of changes in body chemical composition using Fourier transform infrared (FTIR) and changes in cryoprotectant content using high performance liquid chromatography (HPLC), we showed how aphid C. tujafilina adapted to overwintering as an active stage.
33419222	1	3	theme	consequences	155:166	arg1	One					144:146	One	144:146	One	144:146	One of the consequences of climate change is the expansion of insects' ranges.
33419222	1	3	theme	consequences	155:166	arg1	consequences					155:166	the consequences	151:166	the consequences of climate change	151:184	One of the consequences of climate change is the expansion of insects' ranges.
33419222	1	3	theme	consequences	155:166	arg1	expansion					193:201	the expansion	189:201	the expansion of insects' ranges	189:220	One of the consequences of climate change is the expansion of insects' ranges.
33419222	5	4	theme	chemical	642:649	arg1	composition					651:661	body chemical composition	637:661	body chemical composition using Fourier transform infrared (FTIR)	637:701	Based on analyses of changes in body chemical composition using Fourier transform infrared (FTIR) and changes in cryoprotectant content using high performance liquid chromatography (HPLC), we showed how aphid C. tujafilina adapted to overwintering as an active stage.
33419222	2	5	from	temperatures	307:318	arg1	winter					323:328	winter	323:328	winter	323:328	Colonization of new habitats forces insects to adapt to new conditions, such as low temperatures in winter.
33419222	8	6	theme	low	1290:1292	arg1	temperatures					1294:1305	unfavorable low temperatures	1278:1305	unfavorable low temperatures	1278:1305	The complex sugar-polyol cryoprotectant system facilitates aphids' survival in unfavorable low temperatures.
33419222	5	7	from	changes	707:713	arg1	content					733:739	cryoprotectant content	718:739	cryoprotectant content using high performance liquid chromatography (HPLC)	718:791	Based on analyses of changes in body chemical composition using Fourier transform infrared (FTIR) and changes in cryoprotectant content using high performance liquid chromatography (HPLC), we showed how aphid C. tujafilina adapted to overwintering as an active stage.
33419222	5	7	from	changes	707:713	arg1	composition					651:661	body chemical composition	637:661	body chemical composition using Fourier transform infrared (FTIR)	637:701	Based on analyses of changes in body chemical composition using Fourier transform infrared (FTIR) and changes in cryoprotectant content using high performance liquid chromatography (HPLC), we showed how aphid C. tujafilina adapted to overwintering as an active stage.
33419222	0	8	theme	Aphid	102:106	arg1	tujafilina					115:124	Anholocyclic Aphid Cinara tujafilina	89:124	Anholocyclic Aphid Cinara tujafilina	89:124	Changes in Chemical Composition and Accumulation of Cryoprotectants as the Adaptation of Anholocyclic Aphid Cinara tujafilina to Overwintering.
33419222	0	9	from	Changes	0:6	arg1	Accumulation					36:47	Accumulation	36:47	Accumulation of Cryoprotectants	36:66	Changes in Chemical Composition and Accumulation of Cryoprotectants as the Adaptation of Anholocyclic Aphid Cinara tujafilina to Overwintering.
33419222	0	9	from	Changes	0:6	arg1	Composition					20:30	Chemical Composition	11:30	Chemical Composition	11:30	Changes in Chemical Composition and Accumulation of Cryoprotectants as the Adaptation of Anholocyclic Aphid Cinara tujafilina to Overwintering.
33419222	2	10	theme	new	279:281	arg1	temperatures					307:318	low temperatures	303:318	low temperatures in winter	303:328	Colonization of new habitats forces insects to adapt to new conditions, such as low temperatures in winter.
33419222	2	10	theme	new	279:281	arg1	conditions					283:292	new conditions	279:292	new conditions	279:292	Colonization of new habitats forces insects to adapt to new conditions, such as low temperatures in winter.
33419222	4	11	theme	colder	589:594	arg1	winters					596:602	surviving colder winters	579:602	surviving colder winters	579:602	On the areas where the populations of C. tujafilina had expanded, it demonstrated its adaptation for surviving colder winters.
33419222	0	12	theme	Anholocyclic	89:100	arg1	tujafilina					115:124	Anholocyclic Aphid Cinara tujafilina	89:124	Anholocyclic Aphid Cinara tujafilina	89:124	Changes in Chemical Composition and Accumulation of Cryoprotectants as the Adaptation of Anholocyclic Aphid Cinara tujafilina to Overwintering.
33419222	4	13	theme	surviving	579:587	arg1	winters					596:602	surviving colder winters	579:602	surviving colder winters	579:602	On the areas where the populations of C. tujafilina had expanded, it demonstrated its adaptation for surviving colder winters.
33419222	0	14	theme	tujafilina	115:124	arg1	Adaptation					75:84	the Adaptation	71:84	the Adaptation of Anholocyclic Aphid Cinara tujafilina to Overwintering	71:141	Changes in Chemical Composition and Accumulation of Cryoprotectants as the Adaptation of Anholocyclic Aphid Cinara tujafilina to Overwintering.
33419222	1	15	theme	climate	171:177	arg1	change					179:184	climate change	171:184	climate change	171:184	One of the consequences of climate change is the expansion of insects' ranges.
33419222	0	16	theme	Cinara	108:113	arg1	tujafilina					115:124	Anholocyclic Aphid Cinara tujafilina	89:124	Anholocyclic Aphid Cinara tujafilina	89:124	Changes in Chemical Composition and Accumulation of Cryoprotectants as the Adaptation of Anholocyclic Aphid Cinara tujafilina to Overwintering.
33419222	5	17	dep	Fourier	669:675	arg1	transform					677:685	transform	677:685	transform infrared (FTIR)	677:701	Based on analyses of changes in body chemical composition using Fourier transform infrared (FTIR) and changes in cryoprotectant content using high performance liquid chromatography (HPLC), we showed how aphid C. tujafilina adapted to overwintering as an active stage.
33419222	7	18	theme	main	1125:1128	arg1	glycerol					1069:1076	glycerol	1069:1076	glycerol	1069:1076	Glucose, trehalose, mannitol, myo-inositol and glycerol were identified in the aphid body in winter as main putative cryoprotectants to increase the insects' tolerance to cold.
33419222	7	18	theme	main	1125:1128	arg1	mannitol					1042:1049	mannitol	1042:1049	mannitol	1042:1049	Glucose, trehalose, mannitol, myo-inositol and glycerol were identified in the aphid body in winter as main putative cryoprotectants to increase the insects' tolerance to cold.
33419222	7	18	theme	main	1125:1128	arg1	myo-inositol					1052:1063	myo-inositol	1052:1063	myo-inositol	1052:1063	Glucose, trehalose, mannitol, myo-inositol and glycerol were identified in the aphid body in winter as main putative cryoprotectants to increase the insects' tolerance to cold.
33419222	7	18	theme	main	1125:1128	arg1	Glucose					1022:1028	Glucose	1022:1028	Glucose	1022:1028	Glucose, trehalose, mannitol, myo-inositol and glycerol were identified in the aphid body in winter as main putative cryoprotectants to increase the insects' tolerance to cold.
33419222	7	18	theme	main	1125:1128	arg1	cryoprotectants					1139:1153	main putative cryoprotectants	1125:1153	main putative cryoprotectants	1125:1153	Glucose, trehalose, mannitol, myo-inositol and glycerol were identified in the aphid body in winter as main putative cryoprotectants to increase the insects' tolerance to cold.
33419222	7	18	theme	main	1125:1128	arg1	trehalose					1031:1039	trehalose	1031:1039	trehalose	1031:1039	Glucose, trehalose, mannitol, myo-inositol and glycerol were identified in the aphid body in winter as main putative cryoprotectants to increase the insects' tolerance to cold.
33419222	1	19	theme	change	179:184	arg1	consequences					155:166	the consequences	151:166	the consequences of climate change	151:184	One of the consequences of climate change is the expansion of insects' ranges.
33419222	3	20	theme	anholocyclic	367:378	arg1	tujafilina					338:347	Cinara tujafilina	331:347	Cinara tujafilina	331:347	Cinara tujafilina is a thermophilic anholocyclic aphid species, which reproduce exclusively parthenogenetic throughout the year, including winter.
33419222	3	20	theme	anholocyclic	367:378	arg1	species					386:392	a thermophilic anholocyclic aphid species	352:392	a thermophilic anholocyclic aphid species	352:392	Cinara tujafilina is a thermophilic anholocyclic aphid species, which reproduce exclusively parthenogenetic throughout the year, including winter.
33419222	5	21	theme	changes	626:632	arg1	analyses					614:621	analyses	614:621	analyses of changes in body chemical composition using Fourier transform infrared (FTIR) and changes in cryoprotectant content using high performance liquid chromatography (HPLC)	614:791	Based on analyses of changes in body chemical composition using Fourier transform infrared (FTIR) and changes in cryoprotectant content using high performance liquid chromatography (HPLC), we showed how aphid C. tujafilina adapted to overwintering as an active stage.
33419222	6	22	theme	peak	938:941	arg1	values					943:948	higher peak values	931:948	higher peak values originating from the carbohydrates, proteins and lipids,	931:1005	In the FTIR spectrum of the winter type of C. tujafilina, higher peak values originating from the carbohydrates, proteins and lipids, were observed.
33419222	3	23	theme	aphid	380:384	arg1	tujafilina					338:347	Cinara tujafilina	331:347	Cinara tujafilina	331:347	Cinara tujafilina is a thermophilic anholocyclic aphid species, which reproduce exclusively parthenogenetic throughout the year, including winter.
33419222	3	23	theme	aphid	380:384	arg1	species					386:392	a thermophilic anholocyclic aphid species	352:392	a thermophilic anholocyclic aphid species	352:392	Cinara tujafilina is a thermophilic anholocyclic aphid species, which reproduce exclusively parthenogenetic throughout the year, including winter.
33419222	5	24	from	changes	626:632	arg1	content					733:739	cryoprotectant content	718:739	cryoprotectant content using high performance liquid chromatography (HPLC)	718:791	Based on analyses of changes in body chemical composition using Fourier transform infrared (FTIR) and changes in cryoprotectant content using high performance liquid chromatography (HPLC), we showed how aphid C. tujafilina adapted to overwintering as an active stage.
33419222	5	24	from	changes	626:632	arg1	composition					651:661	body chemical composition	637:661	body chemical composition using Fourier transform infrared (FTIR)	637:701	Based on analyses of changes in body chemical composition using Fourier transform infrared (FTIR) and changes in cryoprotectant content using high performance liquid chromatography (HPLC), we showed how aphid C. tujafilina adapted to overwintering as an active stage.
33419222	5	25	theme	active	859:864	arg1	stage					866:870	an active stage	856:870	an active stage	856:870	Based on analyses of changes in body chemical composition using Fourier transform infrared (FTIR) and changes in cryoprotectant content using high performance liquid chromatography (HPLC), we showed how aphid C. tujafilina adapted to overwintering as an active stage.
33419222	6	26	theme	higher	931:936	arg1	values					943:948	higher peak values	931:948	higher peak values originating from the carbohydrates, proteins and lipids,	931:1005	In the FTIR spectrum of the winter type of C. tujafilina, higher peak values originating from the carbohydrates, proteins and lipids, were observed.
33419222	8	27	from	survival	1266:1273	arg1	temperatures					1294:1305	unfavorable low temperatures	1278:1305	unfavorable low temperatures	1278:1305	The complex sugar-polyol cryoprotectant system facilitates aphids' survival in unfavorable low temperatures.
33419222	5	28	theme	C.	814:815	arg1	tujafilina					817:826	aphid C. tujafilina	808:826	aphid C. tujafilina	808:826	Based on analyses of changes in body chemical composition using Fourier transform infrared (FTIR) and changes in cryoprotectant content using high performance liquid chromatography (HPLC), we showed how aphid C. tujafilina adapted to overwintering as an active stage.
33419222	3	29	theme	thermophilic	354:365	arg1	tujafilina					338:347	Cinara tujafilina	331:347	Cinara tujafilina	331:347	Cinara tujafilina is a thermophilic anholocyclic aphid species, which reproduce exclusively parthenogenetic throughout the year, including winter.
33419222	3	29	theme	thermophilic	354:365	arg1	species					386:392	a thermophilic anholocyclic aphid species	352:392	a thermophilic anholocyclic aphid species	352:392	Cinara tujafilina is a thermophilic anholocyclic aphid species, which reproduce exclusively parthenogenetic throughout the year, including winter.
33419222	5	30	from	analyses	614:621	arg1	content					733:739	cryoprotectant content	718:739	cryoprotectant content using high performance liquid chromatography (HPLC)	718:791	Based on analyses of changes in body chemical composition using Fourier transform infrared (FTIR) and changes in cryoprotectant content using high performance liquid chromatography (HPLC), we showed how aphid C. tujafilina adapted to overwintering as an active stage.
33419222	5	30	from	analyses	614:621	arg1	composition					651:661	body chemical composition	637:661	body chemical composition using Fourier transform infrared (FTIR)	637:701	Based on analyses of changes in body chemical composition using Fourier transform infrared (FTIR) and changes in cryoprotectant content using high performance liquid chromatography (HPLC), we showed how aphid C. tujafilina adapted to overwintering as an active stage.
33419222	5	31	from	content	733:739	arg1	analyses					614:621	analyses	614:621	analyses of changes in body chemical composition using Fourier transform infrared (FTIR) and changes in cryoprotectant content using high performance liquid chromatography (HPLC)	614:791	Based on analyses of changes in body chemical composition using Fourier transform infrared (FTIR) and changes in cryoprotectant content using high performance liquid chromatography (HPLC), we showed how aphid C. tujafilina adapted to overwintering as an active stage.
33419222	8	32	theme	complex	1203:1209	arg1	system					1239:1244	The complex sugar-polyol cryoprotectant system	1199:1244	The complex sugar-polyol cryoprotectant system	1199:1244	The complex sugar-polyol cryoprotectant system facilitates aphids' survival in unfavorable low temperatures.
33419222	7	33	theme	aphid	1101:1105	arg1	body					1107:1110	the aphid body	1097:1110	the aphid body	1097:1110	Glucose, trehalose, mannitol, myo-inositol and glycerol were identified in the aphid body in winter as main putative cryoprotectants to increase the insects' tolerance to cold.
33419222	6	34	theme	tujafilina	919:928	arg1	type					908:911	the winter type	897:911	the winter type of C. tujafilina	897:928	In the FTIR spectrum of the winter type of C. tujafilina, higher peak values originating from the carbohydrates, proteins and lipids, were observed.
33419222	0	35	theme	Chemical	11:18	arg1	Composition					20:30	Chemical Composition	11:30	Chemical Composition	11:30	Changes in Chemical Composition and Accumulation of Cryoprotectants as the Adaptation of Anholocyclic Aphid Cinara tujafilina to Overwintering.
33419222	4	36	theme	tujafilina	519:528	arg1	populations					501:511	the populations	497:511	the populations of C. tujafilina	497:528	On the areas where the populations of C. tujafilina had expanded, it demonstrated its adaptation for surviving colder winters.
33419222	5	37	theme	changes	707:713	arg1	analyses					614:621	analyses	614:621	analyses of changes in body chemical composition using Fourier transform infrared (FTIR) and changes in cryoprotectant content using high performance liquid chromatography (HPLC)	614:791	Based on analyses of changes in body chemical composition using Fourier transform infrared (FTIR) and changes in cryoprotectant content using high performance liquid chromatography (HPLC), we showed how aphid C. tujafilina adapted to overwintering as an active stage.
33419222	3	38	theme	Cinara	331:336	arg1	tujafilina					338:347	Cinara tujafilina	331:347	Cinara tujafilina	331:347	Cinara tujafilina is a thermophilic anholocyclic aphid species, which reproduce exclusively parthenogenetic throughout the year, including winter.
33419222	3	38	theme	Cinara	331:336	arg1	species					386:392	a thermophilic anholocyclic aphid species	352:392	a thermophilic anholocyclic aphid species	352:392	Cinara tujafilina is a thermophilic anholocyclic aphid species, which reproduce exclusively parthenogenetic throughout the year, including winter.
33419222	0	39	theme	Cryoprotectants	52:66	arg1	Accumulation					36:47	Accumulation	36:47	Accumulation of Cryoprotectants	36:66	Changes in Chemical Composition and Accumulation of Cryoprotectants as the Adaptation of Anholocyclic Aphid Cinara tujafilina to Overwintering.
33419222	0	39	theme	Cryoprotectants	52:66	arg1	Composition					20:30	Chemical Composition	11:30	Chemical Composition	11:30	Changes in Chemical Composition and Accumulation of Cryoprotectants as the Adaptation of Anholocyclic Aphid Cinara tujafilina to Overwintering.
33419222	5	40	from	composition	651:661	arg1	analyses					614:621	analyses	614:621	analyses of changes in body chemical composition using Fourier transform infrared (FTIR) and changes in cryoprotectant content using high performance liquid chromatography (HPLC)	614:791	Based on analyses of changes in body chemical composition using Fourier transform infrared (FTIR) and changes in cryoprotectant content using high performance liquid chromatography (HPLC), we showed how aphid C. tujafilina adapted to overwintering as an active stage.
33419222	6	41	theme	FTIR	880:883	arg1	spectrum					885:892	the FTIR spectrum	876:892	the FTIR spectrum of the winter type of C. tujafilina	876:928	In the FTIR spectrum of the winter type of C. tujafilina, higher peak values originating from the carbohydrates, proteins and lipids, were observed.
33419222	6	42	theme	type	908:911	arg1	spectrum					885:892	the FTIR spectrum	876:892	the FTIR spectrum of the winter type of C. tujafilina	876:928	In the FTIR spectrum of the winter type of C. tujafilina, higher peak values originating from the carbohydrates, proteins and lipids, were observed.
33419222	6	43	theme	winter	901:906	arg1	type					908:911	the winter type	897:911	the winter type of C. tujafilina	897:928	In the FTIR spectrum of the winter type of C. tujafilina, higher peak values originating from the carbohydrates, proteins and lipids, were observed.
33419222	5	44	theme	aphid	808:812	arg1	tujafilina					817:826	aphid C. tujafilina	808:826	aphid C. tujafilina	808:826	Based on analyses of changes in body chemical composition using Fourier transform infrared (FTIR) and changes in cryoprotectant content using high performance liquid chromatography (HPLC), we showed how aphid C. tujafilina adapted to overwintering as an active stage.
33419222	2	45	theme	low	303:305	arg1	temperatures					307:318	low temperatures	303:318	low temperatures in winter	303:328	Colonization of new habitats forces insects to adapt to new conditions, such as low temperatures in winter.
33419222	5	46	theme	high	747:750	arg1	HPLC					787:790	HPLC	787:790	HPLC	787:790	Based on analyses of changes in body chemical composition using Fourier transform infrared (FTIR) and changes in cryoprotectant content using high performance liquid chromatography (HPLC), we showed how aphid C. tujafilina adapted to overwintering as an active stage.
33419222	5	46	theme	high	747:750	arg1	chromatography					771:784	high performance liquid chromatography	747:784	high performance liquid chromatography (HPLC)	747:791	Based on analyses of changes in body chemical composition using Fourier transform infrared (FTIR) and changes in cryoprotectant content using high performance liquid chromatography (HPLC), we showed how aphid C. tujafilina adapted to overwintering as an active stage.
33419222	8	47	theme	cryoprotectant	1224:1237	arg1	system					1239:1244	The complex sugar-polyol cryoprotectant system	1199:1244	The complex sugar-polyol cryoprotectant system	1199:1244	The complex sugar-polyol cryoprotectant system facilitates aphids' survival in unfavorable low temperatures.
33419222	2	48	theme	habitats	243:250	arg1	Colonization					223:234	Colonization	223:234	Colonization of new habitats	223:250	Colonization of new habitats forces insects to adapt to new conditions, such as low temperatures in winter.
33419222	8	49	theme	sugar-polyol	1211:1222	arg1	system					1239:1244	The complex sugar-polyol cryoprotectant system	1199:1244	The complex sugar-polyol cryoprotectant system	1199:1244	The complex sugar-polyol cryoprotectant system facilitates aphids' survival in unfavorable low temperatures.
33419222	8	50	theme	unfavorable	1278:1288	arg1	temperatures					1294:1305	unfavorable low temperatures	1278:1305	unfavorable low temperatures	1278:1305	The complex sugar-polyol cryoprotectant system facilitates aphids' survival in unfavorable low temperatures.
33419222	5	51	dep	infrared	687:694	arg1	FTIR					697:700	FTIR	697:700	FTIR	697:700	Based on analyses of changes in body chemical composition using Fourier transform infrared (FTIR) and changes in cryoprotectant content using high performance liquid chromatography (HPLC), we showed how aphid C. tujafilina adapted to overwintering as an active stage.
33419222	5	52	theme	performance	752:762	arg1	HPLC					787:790	HPLC	787:790	HPLC	787:790	Based on analyses of changes in body chemical composition using Fourier transform infrared (FTIR) and changes in cryoprotectant content using high performance liquid chromatography (HPLC), we showed how aphid C. tujafilina adapted to overwintering as an active stage.
33419222	5	52	theme	performance	752:762	arg1	chromatography					771:784	high performance liquid chromatography	747:784	high performance liquid chromatography (HPLC)	747:791	Based on analyses of changes in body chemical composition using Fourier transform infrared (FTIR) and changes in cryoprotectant content using high performance liquid chromatography (HPLC), we showed how aphid C. tujafilina adapted to overwintering as an active stage.
33419222	7	53	theme	putative	1130:1137	arg1	glycerol					1069:1076	glycerol	1069:1076	glycerol	1069:1076	Glucose, trehalose, mannitol, myo-inositol and glycerol were identified in the aphid body in winter as main putative cryoprotectants to increase the insects' tolerance to cold.
33419222	7	53	theme	putative	1130:1137	arg1	mannitol					1042:1049	mannitol	1042:1049	mannitol	1042:1049	Glucose, trehalose, mannitol, myo-inositol and glycerol were identified in the aphid body in winter as main putative cryoprotectants to increase the insects' tolerance to cold.
33419222	7	53	theme	putative	1130:1137	arg1	myo-inositol					1052:1063	myo-inositol	1052:1063	myo-inositol	1052:1063	Glucose, trehalose, mannitol, myo-inositol and glycerol were identified in the aphid body in winter as main putative cryoprotectants to increase the insects' tolerance to cold.
33419222	7	53	theme	putative	1130:1137	arg1	Glucose					1022:1028	Glucose	1022:1028	Glucose	1022:1028	Glucose, trehalose, mannitol, myo-inositol and glycerol were identified in the aphid body in winter as main putative cryoprotectants to increase the insects' tolerance to cold.
33419222	7	53	theme	putative	1130:1137	arg1	cryoprotectants					1139:1153	main putative cryoprotectants	1125:1153	main putative cryoprotectants	1125:1153	Glucose, trehalose, mannitol, myo-inositol and glycerol were identified in the aphid body in winter as main putative cryoprotectants to increase the insects' tolerance to cold.
33419222	7	53	theme	putative	1130:1137	arg1	trehalose					1031:1039	trehalose	1031:1039	trehalose	1031:1039	Glucose, trehalose, mannitol, myo-inositol and glycerol were identified in the aphid body in winter as main putative cryoprotectants to increase the insects' tolerance to cold.
33419222	2	54	theme	new	239:241	arg1	habitats					243:250	new habitats	239:250	new habitats	239:250	Colonization of new habitats forces insects to adapt to new conditions, such as low temperatures in winter.
33419222	5	55	theme	cryoprotectant	718:731	arg1	content					733:739	cryoprotectant content	718:739	cryoprotectant content using high performance liquid chromatography (HPLC)	718:791	Based on analyses of changes in body chemical composition using Fourier transform infrared (FTIR) and changes in cryoprotectant content using high performance liquid chromatography (HPLC), we showed how aphid C. tujafilina adapted to overwintering as an active stage.
32710966	7	0	theme	intestinal	1774:1783	arg1	barrier					1785:1791	intestinal barrier	1774:1791	intestinal barrier	1774:1791	In conclusion, pre-administration of PM could provide neuroprotective effects against PD pathogenesis by suppressing inflammation in gut, brain and systemic circulation, and by improving integrity of intestinal barrier and BBB.
32710966	4	1	theme	protein	1046:1052	arg1	MAPK					1063:1066	MAPK	1063:1066	MAPK	1063:1066	PM significantly alleviated inflammation in gut, brain and systemic circulation as shown by reduced levels or expressions of pro-inflammatory cytokines concurrently and inhibited mitogen-activated protein kinases (MAPK) signaling pathway in mice colon.
32710966	4	1	theme	protein	1046:1052	arg1	kinases					1054:1060	mitogen-activated protein kinases	1028:1060	mitogen-activated protein kinases (MAPK) signaling pathway	1028:1085	PM significantly alleviated inflammation in gut, brain and systemic circulation as shown by reduced levels or expressions of pro-inflammatory cytokines concurrently and inhibited mitogen-activated protein kinases (MAPK) signaling pathway in mice colon.
32710966	5	2	dep	mice	1279:1282	arg1	SNpc					1294:1297	SNpc	1294:1297	SNpc	1294:1297	Meanwhile, PM greatly improved integrity of intestinal barrier and blood brain barrier (BBB) as indicated by increased expressions of tight junction associated proteins in both mice colon and SNpc.
32710966	5	2	dep	mice	1279:1282	arg1	colon					1284:1288	colon	1284:1288	colon	1284:1288	Meanwhile, PM greatly improved integrity of intestinal barrier and blood brain barrier (BBB) as indicated by increased expressions of tight junction associated proteins in both mice colon and SNpc.
32710966	5	2	dep	mice	1279:1282	arg1	mice					1279:1282	both mice colon and SNpc	1274:1297	both mice colon and SNpc	1274:1297	Meanwhile, PM greatly improved integrity of intestinal barrier and blood brain barrier (BBB) as indicated by increased expressions of tight junction associated proteins in both mice colon and SNpc.
32710966	6	3	theme	chain	1523:1527	arg1	SCFAs					1542:1546	SCFAs	1542:1546	SCFAs	1542:1546	Further studies indicated PM treatment resulted in changes of gut microbial compositions, together with great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice.
32710966	6	3	theme	chain	1523:1527	arg1	acids					1535:1539	fecal short chain fatty acids	1511:1539	fecal short chain fatty acids (SCFAs)	1511:1547	Further studies indicated PM treatment resulted in changes of gut microbial compositions, together with great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice.
32710966	3	4	theme	homovanillic	726:737	arg1	acid					739:742	striatal homovanillic acid	717:742	striatal homovanillic acid (HVA)	717:748	The results showed PM administration improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc) and enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice.
32710966	3	4	theme	homovanillic	726:737	arg1	HVA					745:747	HVA	745:747	HVA	745:747	The results showed PM administration improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc) and enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice.
32710966	6	5	theme	digestion	1425:1433	arg1	alterations					1410:1420	great alterations	1404:1420	great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice	1404:1571	Further studies indicated PM treatment resulted in changes of gut microbial compositions, together with great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice.
32710966	6	6	theme	fecal	1511:1515	arg1	SCFAs					1542:1546	SCFAs	1542:1546	SCFAs	1542:1546	Further studies indicated PM treatment resulted in changes of gut microbial compositions, together with great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice.
32710966	6	6	theme	fecal	1511:1515	arg1	acids					1535:1539	fecal short chain fatty acids	1511:1539	fecal short chain fatty acids (SCFAs)	1511:1547	Further studies indicated PM treatment resulted in changes of gut microbial compositions, together with great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice.
32710966	1	7	theme	underlying	190:199	arg1	mechanisms					201:210	underlying mechanisms	190:210	underlying mechanisms for brown seaweed polysaccharide	190:243	The study aims to investigate the potentially neuroprotective effects and underlying mechanisms for brown seaweed polysaccharide of polymannuronic acid (PM) against Parkinson's disease (PD) pathogenesis.
32710966	8	8	theme	derived	1884:1890	arg1	SCFAs					1892:1896	gut microbiota derived SCFAs	1869:1896	gut microbiota derived SCFAs as mediators	1869:1909	PM might modulate brain-gut-microbiota axis, at least in part, via gut microbiota derived SCFAs as mediators.
32710966	3	9	theme	serotonin	751:759	arg1	contents					705:712	contents	705:712	contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA)	705:835	The results showed PM administration improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc) and enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice.
32710966	2	10	theme	PD	320:321	arg1	mice					329:332	PD model mice	320:332	PD model mice	320:332	PD model mice were pretreated with PM via oral gavage once per day for 4 weeks and the preventative effects of PM against neuronal loss together with its modulation on brain-gut-microbiota axis were systematically explored.
32710966	7	11	theme	systemic	1722:1729	arg1	circulation					1731:1741	systemic circulation	1722:1741	systemic circulation	1722:1741	In conclusion, pre-administration of PM could provide neuroprotective effects against PD pathogenesis by suppressing inflammation in gut, brain and systemic circulation, and by improving integrity of intestinal barrier and BBB.
32710966	5	12	from	proteins	1262:1269	arg1	SNpc					1294:1297	SNpc	1294:1297	SNpc	1294:1297	Meanwhile, PM greatly improved integrity of intestinal barrier and blood brain barrier (BBB) as indicated by increased expressions of tight junction associated proteins in both mice colon and SNpc.
32710966	5	12	from	proteins	1262:1269	arg1	colon					1284:1288	colon	1284:1288	colon	1284:1288	Meanwhile, PM greatly improved integrity of intestinal barrier and blood brain barrier (BBB) as indicated by increased expressions of tight junction associated proteins in both mice colon and SNpc.
32710966	5	12	from	proteins	1262:1269	arg1	mice					1279:1282	both mice colon and SNpc	1274:1297	both mice colon and SNpc	1274:1297	Meanwhile, PM greatly improved integrity of intestinal barrier and blood brain barrier (BBB) as indicated by increased expressions of tight junction associated proteins in both mice colon and SNpc.
32710966	4	13	theme	mice	1090:1093	arg1	colon					1095:1099	mice colon	1090:1099	mice colon	1090:1099	PM significantly alleviated inflammation in gut, brain and systemic circulation as shown by reduced levels or expressions of pro-inflammatory cytokines concurrently and inhibited mitogen-activated protein kinases (MAPK) signaling pathway in mice colon.
32710966	1	14	theme	seaweed	222:228	arg1	polysaccharide					230:243	brown seaweed polysaccharide	216:243	brown seaweed polysaccharide	216:243	The study aims to investigate the potentially neuroprotective effects and underlying mechanisms for brown seaweed polysaccharide of polymannuronic acid (PM) against Parkinson's disease (PD) pathogenesis.
32710966	6	15	theme	Further	1300:1306	arg1	studies					1308:1314	Further studies	1300:1314	Further studies	1300:1314	Further studies indicated PM treatment resulted in changes of gut microbial compositions, together with great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice.
32710966	3	16	theme	dopaminergic	620:631	arg1	loss					642:645	dopaminergic neuronal loss	620:645	dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc)	620:690	The results showed PM administration improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc) and enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice.
32710966	0	17	from	axis	81:84	arg1	model					109:113	Parkinson's disease model	89:113	Parkinson's disease model	89:113	Polymannuronic acid prevents dopaminergic neuronal loss via brain-gut-microbiota axis in Parkinson's disease model.
32710966	5	18	from	expressions	1221:1231	arg1	SNpc					1294:1297	SNpc	1294:1297	SNpc	1294:1297	Meanwhile, PM greatly improved integrity of intestinal barrier and blood brain barrier (BBB) as indicated by increased expressions of tight junction associated proteins in both mice colon and SNpc.
32710966	5	18	from	expressions	1221:1231	arg1	colon					1284:1288	colon	1284:1288	colon	1284:1288	Meanwhile, PM greatly improved integrity of intestinal barrier and blood brain barrier (BBB) as indicated by increased expressions of tight junction associated proteins in both mice colon and SNpc.
32710966	5	18	from	expressions	1221:1231	arg1	mice					1279:1282	both mice colon and SNpc	1274:1297	both mice colon and SNpc	1274:1297	Meanwhile, PM greatly improved integrity of intestinal barrier and blood brain barrier (BBB) as indicated by increased expressions of tight junction associated proteins in both mice colon and SNpc.
32710966	3	19	dep	showed	556:561	arg1	enhanced					696:703	enhanced	696:703	enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice	696:846	The results showed PM administration improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc) and enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice.
32710966	3	19	dep	showed	556:561	arg1	improved					581:588	improved	581:588	improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc)	581:690	The results showed PM administration improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc) and enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice.
32710966	4	20	theme	reduced	941:947	arg1	levels					949:954	reduced levels	941:954	reduced levels	941:954	PM significantly alleviated inflammation in gut, brain and systemic circulation as shown by reduced levels or expressions of pro-inflammatory cytokines concurrently and inhibited mitogen-activated protein kinases (MAPK) signaling pathway in mice colon.
32710966	3	21	theme	acetic	785:790	arg1	5-HIAA					798:803	5-HIAA	798:803	5-HIAA	798:803	The results showed PM administration improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc) and enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice.
32710966	3	21	theme	acetic	785:790	arg1	acid					792:795	5-hydroxyindole acetic acid	769:795	5-hydroxyindole acetic acid (5-HIAA)	769:804	The results showed PM administration improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc) and enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice.
32710966	6	22	theme	microbial	1366:1374	arg1	compositions					1376:1387	gut microbial compositions	1362:1387	gut microbial compositions	1362:1387	Further studies indicated PM treatment resulted in changes of gut microbial compositions, together with great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice.
32710966	6	23	theme	acids	1535:1539	arg1	increase					1499:1506	surge increase	1493:1506	surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice	1493:1571	Further studies indicated PM treatment resulted in changes of gut microbial compositions, together with great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice.
32710966	1	24	theme	PD	302:303	arg1	pathogenesis					306:317	Parkinson's disease (PD) pathogenesis	281:317	Parkinson's disease (PD) pathogenesis	281:317	The study aims to investigate the potentially neuroprotective effects and underlying mechanisms for brown seaweed polysaccharide of polymannuronic acid (PM) against Parkinson's disease (PD) pathogenesis.
32710966	7	25	dep	provide	1620:1626	arg1	suppressing					1679:1689	suppressing	1679:1689	suppressing inflammation in gut, brain and systemic circulation	1679:1741	In conclusion, pre-administration of PM could provide neuroprotective effects against PD pathogenesis by suppressing inflammation in gut, brain and systemic circulation, and by improving integrity of intestinal barrier and BBB.
32710966	7	25	dep	provide	1620:1626	arg1	improving					1751:1759	improving	1751:1759	improving integrity of intestinal barrier and BBB	1751:1799	In conclusion, pre-administration of PM could provide neuroprotective effects against PD pathogenesis by suppressing inflammation in gut, brain and systemic circulation, and by improving integrity of intestinal barrier and BBB.
32710966	5	26	theme	tight	1236:1240	arg1	proteins					1262:1269	tight junction associated proteins	1236:1269	tight junction associated proteins in both mice colon and SNpc	1236:1297	Meanwhile, PM greatly improved integrity of intestinal barrier and blood brain barrier (BBB) as indicated by increased expressions of tight junction associated proteins in both mice colon and SNpc.
32710966	3	27	theme	γ-aminobutyric	810:823	arg1	GABA					831:834	GABA	831:834	GABA	831:834	The results showed PM administration improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc) and enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice.
32710966	3	27	theme	γ-aminobutyric	810:823	arg1	acid					825:828	γ-aminobutyric acid	810:828	γ-aminobutyric acid (GABA)	810:835	The results showed PM administration improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc) and enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice.
32710966	3	28	theme	PD	840:841	arg1	mice					843:846	PD mice	840:846	PD mice	840:846	The results showed PM administration improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc) and enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice.
32710966	5	29	theme	associated	1251:1260	arg1	proteins					1262:1269	tight junction associated proteins	1236:1269	tight junction associated proteins in both mice colon and SNpc	1236:1297	Meanwhile, PM greatly improved integrity of intestinal barrier and blood brain barrier (BBB) as indicated by increased expressions of tight junction associated proteins in both mice colon and SNpc.
32710966	0	30	theme	disease	101:107	arg1	model					109:113	Parkinson's disease model	89:113	Parkinson's disease model	89:113	Polymannuronic acid prevents dopaminergic neuronal loss via brain-gut-microbiota axis in Parkinson's disease model.
32710966	2	31	dep	together	456:463	arg1	with					465:468	with	465:468	with	465:468	PD model mice were pretreated with PM via oral gavage once per day for 4 weeks and the preventative effects of PM against neuronal loss together with its modulation on brain-gut-microbiota axis were systematically explored.
32710966	3	32	from	loss	642:645	arg1	SNpc					686:689	SNpc	686:689	SNpc	686:689	The results showed PM administration improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc) and enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice.
32710966	3	32	from	loss	642:645	arg1	compacta					676:683	the substantia nigra pars compacta	650:683	the substantia nigra pars compacta (SNpc)	650:690	The results showed PM administration improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc) and enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice.
32710966	7	33	theme	neuroprotective	1628:1642	arg1	effects					1644:1650	neuroprotective effects	1628:1650	neuroprotective effects	1628:1650	In conclusion, pre-administration of PM could provide neuroprotective effects against PD pathogenesis by suppressing inflammation in gut, brain and systemic circulation, and by improving integrity of intestinal barrier and BBB.
32710966	2	34	theme	brain-gut-microbiota	488:507	arg1	axis					509:512	brain-gut-microbiota axis	488:512	brain-gut-microbiota axis	488:512	PD model mice were pretreated with PM via oral gavage once per day for 4 weeks and the preventative effects of PM against neuronal loss together with its modulation on brain-gut-microbiota axis were systematically explored.
32710966	0	35	theme	Polymannuronic	0:13	arg1	acid					15:18	Polymannuronic acid	0:18	Polymannuronic acid	0:18	Polymannuronic acid prevents dopaminergic neuronal loss via brain-gut-microbiota axis in Parkinson's disease model.
32710966	1	36	theme	disease	293:299	arg1	pathogenesis					306:317	Parkinson's disease (PD) pathogenesis	281:317	Parkinson's disease (PD) pathogenesis	281:317	The study aims to investigate the potentially neuroprotective effects and underlying mechanisms for brown seaweed polysaccharide of polymannuronic acid (PM) against Parkinson's disease (PD) pathogenesis.
32710966	6	37	theme	PD	1565:1566	arg1	mice					1568:1571	PD mice	1565:1571	PD mice	1565:1571	Further studies indicated PM treatment resulted in changes of gut microbial compositions, together with great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice.
32710966	4	38	theme	mitogen-activated	1028:1044	arg1	MAPK					1063:1066	MAPK	1063:1066	MAPK	1063:1066	PM significantly alleviated inflammation in gut, brain and systemic circulation as shown by reduced levels or expressions of pro-inflammatory cytokines concurrently and inhibited mitogen-activated protein kinases (MAPK) signaling pathway in mice colon.
32710966	4	38	theme	mitogen-activated	1028:1044	arg1	kinases					1054:1060	mitogen-activated protein kinases	1028:1060	mitogen-activated protein kinases (MAPK) signaling pathway	1028:1085	PM significantly alleviated inflammation in gut, brain and systemic circulation as shown by reduced levels or expressions of pro-inflammatory cytokines concurrently and inhibited mitogen-activated protein kinases (MAPK) signaling pathway in mice colon.
32710966	2	39	theme	preventative	407:418	arg1	effects					420:426	the preventative effects	403:426	the preventative effects of PM against neuronal loss together with its modulation on brain-gut-microbiota axis	403:512	PD model mice were pretreated with PM via oral gavage once per day for 4 weeks and the preventative effects of PM against neuronal loss together with its modulation on brain-gut-microbiota axis were systematically explored.
32710966	5	40	theme	brain	1175:1179	arg1	BBB					1190:1192	BBB	1190:1192	BBB	1190:1192	Meanwhile, PM greatly improved integrity of intestinal barrier and blood brain barrier (BBB) as indicated by increased expressions of tight junction associated proteins in both mice colon and SNpc.
32710966	5	40	theme	brain	1175:1179	arg1	barrier					1181:1187	blood brain barrier	1169:1187	blood brain barrier (BBB)	1169:1193	Meanwhile, PM greatly improved integrity of intestinal barrier and blood brain barrier (BBB) as indicated by increased expressions of tight junction associated proteins in both mice colon and SNpc.
32710966	8	41	link	derived	1884:1890	arg1	SCFAs					1892:1896	gut microbiota derived SCFAs	1869:1896	gut microbiota derived SCFAs as mediators	1869:1909	PM might modulate brain-gut-microbiota axis, at least in part, via gut microbiota derived SCFAs as mediators.
32710966	0	42	theme	neuronal	42:49	arg1	loss					51:54	dopaminergic neuronal loss	29:54	dopaminergic neuronal loss	29:54	Polymannuronic acid prevents dopaminergic neuronal loss via brain-gut-microbiota axis in Parkinson's disease model.
32710966	4	43	theme	cytokines	991:999	arg1	expressions					959:969	expressions	959:969	expressions	959:969	PM significantly alleviated inflammation in gut, brain and systemic circulation as shown by reduced levels or expressions of pro-inflammatory cytokines concurrently and inhibited mitogen-activated protein kinases (MAPK) signaling pathway in mice colon.
32710966	4	43	theme	cytokines	991:999	arg1	levels					949:954	reduced levels	941:954	reduced levels	941:954	PM significantly alleviated inflammation in gut, brain and systemic circulation as shown by reduced levels or expressions of pro-inflammatory cytokines concurrently and inhibited mitogen-activated protein kinases (MAPK) signaling pathway in mice colon.
32710966	3	44	theme	nigra	665:669	arg1	SNpc					686:689	SNpc	686:689	SNpc	686:689	The results showed PM administration improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc) and enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice.
32710966	3	44	theme	nigra	665:669	arg1	compacta					676:683	the substantia nigra pars compacta	650:683	the substantia nigra pars compacta (SNpc)	650:690	The results showed PM administration improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc) and enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice.
32710966	5	45	theme	intestinal	1146:1155	arg1	barrier					1157:1163	intestinal barrier	1146:1163	intestinal barrier	1146:1163	Meanwhile, PM greatly improved integrity of intestinal barrier and blood brain barrier (BBB) as indicated by increased expressions of tight junction associated proteins in both mice colon and SNpc.
32710966	7	46	theme	BBB	1797:1799	arg1	integrity					1761:1769	integrity	1761:1769	integrity of intestinal barrier and BBB	1761:1799	In conclusion, pre-administration of PM could provide neuroprotective effects against PD pathogenesis by suppressing inflammation in gut, brain and systemic circulation, and by improving integrity of intestinal barrier and BBB.
32710966	4	47	theme	signaling	1069:1077	arg1	pathway					1079:1085	mitogen-activated protein kinases (MAPK) signaling pathway	1028:1085	mitogen-activated protein kinases (MAPK) signaling pathway	1028:1085	PM significantly alleviated inflammation in gut, brain and systemic circulation as shown by reduced levels or expressions of pro-inflammatory cytokines concurrently and inhibited mitogen-activated protein kinases (MAPK) signaling pathway in mice colon.
32710966	1	48	theme	polymannuronic	248:261	arg1	PM					269:270	PM	269:270	PM	269:270	The study aims to investigate the potentially neuroprotective effects and underlying mechanisms for brown seaweed polysaccharide of polymannuronic acid (PM) against Parkinson's disease (PD) pathogenesis.
32710966	1	48	theme	polymannuronic	248:261	arg1	acid					263:266	polymannuronic acid	248:266	polymannuronic acid (PM)	248:271	The study aims to investigate the potentially neuroprotective effects and underlying mechanisms for brown seaweed polysaccharide of polymannuronic acid (PM) against Parkinson's disease (PD) pathogenesis.
32710966	7	49	theme	barrier	1785:1791	arg1	integrity					1761:1769	integrity	1761:1769	integrity of intestinal barrier and BBB	1761:1799	In conclusion, pre-administration of PM could provide neuroprotective effects against PD pathogenesis by suppressing inflammation in gut, brain and systemic circulation, and by improving integrity of intestinal barrier and BBB.
32710966	5	50	theme	increased	1211:1219	arg1	expressions					1221:1231	increased expressions	1211:1231	increased expressions of tight junction associated proteins in both mice colon and SNpc	1211:1297	Meanwhile, PM greatly improved integrity of intestinal barrier and blood brain barrier (BBB) as indicated by increased expressions of tight junction associated proteins in both mice colon and SNpc.
32710966	4	51	theme	kinases	1054:1060	arg1	pathway					1079:1085	mitogen-activated protein kinases (MAPK) signaling pathway	1028:1085	mitogen-activated protein kinases (MAPK) signaling pathway	1028:1085	PM significantly alleviated inflammation in gut, brain and systemic circulation as shown by reduced levels or expressions of pro-inflammatory cytokines concurrently and inhibited mitogen-activated protein kinases (MAPK) signaling pathway in mice colon.
32710966	3	52	theme	striatal	717:724	arg1	acid					739:742	striatal homovanillic acid	717:742	striatal homovanillic acid (HVA)	717:748	The results showed PM administration improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc) and enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice.
32710966	3	52	theme	striatal	717:724	arg1	HVA					745:747	HVA	745:747	HVA	745:747	The results showed PM administration improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc) and enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice.
32710966	8	53	theme	brain-gut-microbiota	1820:1839	arg1	axis					1841:1844	brain-gut-microbiota axis	1820:1844	brain-gut-microbiota axis	1820:1844	PM might modulate brain-gut-microbiota axis, at least in part, via gut microbiota derived SCFAs as mediators.
32710966	6	54	theme	dietary	1453:1459	arg1	proteins					1461:1468	dietary proteins and fats	1453:1477	proteins	1461:1468	Further studies indicated PM treatment resulted in changes of gut microbial compositions, together with great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice.
32710966	6	55	theme	metabolism	1439:1448	arg1	alterations					1410:1420	great alterations	1404:1420	great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice	1404:1571	Further studies indicated PM treatment resulted in changes of gut microbial compositions, together with great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice.
32710966	6	56	theme	short	1517:1521	arg1	SCFAs					1542:1546	SCFAs	1542:1546	SCFAs	1542:1546	Further studies indicated PM treatment resulted in changes of gut microbial compositions, together with great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice.
32710966	6	56	theme	short	1517:1521	arg1	acids					1535:1539	fecal short chain fatty acids	1511:1539	fecal short chain fatty acids (SCFAs)	1511:1547	Further studies indicated PM treatment resulted in changes of gut microbial compositions, together with great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice.
32710966	2	57	theme	neuronal	442:449	arg1	loss					451:454	neuronal loss	442:454	neuronal loss together with its modulation on brain-gut-microbiota axis	442:512	PD model mice were pretreated with PM via oral gavage once per day for 4 weeks and the preventative effects of PM against neuronal loss together with its modulation on brain-gut-microbiota axis were systematically explored.
32710966	7	58	from	inflammation	1691:1702	arg1	circulation					1731:1741	systemic circulation	1722:1741	systemic circulation	1722:1741	In conclusion, pre-administration of PM could provide neuroprotective effects against PD pathogenesis by suppressing inflammation in gut, brain and systemic circulation, and by improving integrity of intestinal barrier and BBB.
32710966	7	58	from	inflammation	1691:1702	arg1	gut					1707:1709	gut	1707:1709	gut	1707:1709	In conclusion, pre-administration of PM could provide neuroprotective effects against PD pathogenesis by suppressing inflammation in gut, brain and systemic circulation, and by improving integrity of intestinal barrier and BBB.
32710966	7	58	from	inflammation	1691:1702	arg1	brain					1712:1716	brain	1712:1716	brain	1712:1716	In conclusion, pre-administration of PM could provide neuroprotective effects against PD pathogenesis by suppressing inflammation in gut, brain and systemic circulation, and by improving integrity of intestinal barrier and BBB.
32710966	3	59	theme	acid	739:742	arg1	contents					705:712	contents	705:712	contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA)	705:835	The results showed PM administration improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc) and enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice.
32710966	2	60	theme	model	323:327	arg1	mice					329:332	PD model mice	320:332	PD model mice	320:332	PD model mice were pretreated with PM via oral gavage once per day for 4 weeks and the preventative effects of PM against neuronal loss together with its modulation on brain-gut-microbiota axis were systematically explored.
32710966	5	61	from	mice	1279:1282	arg1	expressions					1221:1231	increased expressions	1211:1231	increased expressions of tight junction associated proteins in both mice colon and SNpc	1211:1297	Meanwhile, PM greatly improved integrity of intestinal barrier and blood brain barrier (BBB) as indicated by increased expressions of tight junction associated proteins in both mice colon and SNpc.
32710966	6	62	theme	PM	1326:1327	arg1	treatment					1329:1337	PM treatment	1326:1337	PM treatment	1326:1337	Further studies indicated PM treatment resulted in changes of gut microbial compositions, together with great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice.
32710966	3	63	theme	PM	563:564	arg1	administration					566:579	PM administration	563:579	PM administration	563:579	The results showed PM administration improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc) and enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice.
32710966	6	64	theme	surge	1493:1497	arg1	increase					1499:1506	surge increase	1493:1506	surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice	1493:1571	Further studies indicated PM treatment resulted in changes of gut microbial compositions, together with great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice.
32710966	1	65	theme	brown	216:220	arg1	polysaccharide					230:243	brown seaweed polysaccharide	216:243	brown seaweed polysaccharide	216:243	The study aims to investigate the potentially neuroprotective effects and underlying mechanisms for brown seaweed polysaccharide of polymannuronic acid (PM) against Parkinson's disease (PD) pathogenesis.
32710966	3	66	theme	motor	590:594	arg1	functions					596:604	motor functions	590:604	motor functions	590:604	The results showed PM administration improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc) and enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice.
32710966	2	67	from	modulation	474:483	arg1	axis					509:512	brain-gut-microbiota axis	488:512	brain-gut-microbiota axis	488:512	PD model mice were pretreated with PM via oral gavage once per day for 4 weeks and the preventative effects of PM against neuronal loss together with its modulation on brain-gut-microbiota axis were systematically explored.
32710966	3	68	theme	5-hydroxyindole	769:783	arg1	5-HIAA					798:803	5-HIAA	798:803	5-HIAA	798:803	The results showed PM administration improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc) and enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice.
32710966	3	68	theme	5-hydroxyindole	769:783	arg1	acid					792:795	5-hydroxyindole acetic acid	769:795	5-hydroxyindole acetic acid (5-HIAA)	769:804	The results showed PM administration improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc) and enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice.
32710966	5	69	theme	proteins	1262:1269	arg1	expressions					1221:1231	increased expressions	1211:1231	increased expressions of tight junction associated proteins in both mice colon and SNpc	1211:1297	Meanwhile, PM greatly improved integrity of intestinal barrier and blood brain barrier (BBB) as indicated by increased expressions of tight junction associated proteins in both mice colon and SNpc.
32710966	4	70	from	inflammation	877:888	arg1	brain					898:902	brain	898:902	brain	898:902	PM significantly alleviated inflammation in gut, brain and systemic circulation as shown by reduced levels or expressions of pro-inflammatory cytokines concurrently and inhibited mitogen-activated protein kinases (MAPK) signaling pathway in mice colon.
32710966	4	70	from	inflammation	877:888	arg1	circulation					917:927	systemic circulation	908:927	systemic circulation	908:927	PM significantly alleviated inflammation in gut, brain and systemic circulation as shown by reduced levels or expressions of pro-inflammatory cytokines concurrently and inhibited mitogen-activated protein kinases (MAPK) signaling pathway in mice colon.
32710966	4	70	from	inflammation	877:888	arg1	gut					893:895	gut	893:895	gut	893:895	PM significantly alleviated inflammation in gut, brain and systemic circulation as shown by reduced levels or expressions of pro-inflammatory cytokines concurrently and inhibited mitogen-activated protein kinases (MAPK) signaling pathway in mice colon.
32710966	6	71	theme	compositions	1376:1387	arg1	changes					1351:1357	changes	1351:1357	changes of gut microbial compositions	1351:1387	Further studies indicated PM treatment resulted in changes of gut microbial compositions, together with great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice.
32710966	3	72	theme	neuronal	633:640	arg1	loss					642:645	dopaminergic neuronal loss	620:645	dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc)	620:690	The results showed PM administration improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc) and enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice.
32710966	3	73	theme	acid	792:795	arg1	contents					705:712	contents	705:712	contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA)	705:835	The results showed PM administration improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc) and enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice.
32710966	6	74	theme	gut	1362:1364	arg1	compositions					1376:1387	gut microbial compositions	1362:1387	gut microbial compositions	1362:1387	Further studies indicated PM treatment resulted in changes of gut microbial compositions, together with great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice.
32710966	6	75	theme	fatty	1529:1533	arg1	SCFAs					1542:1546	SCFAs	1542:1546	SCFAs	1542:1546	Further studies indicated PM treatment resulted in changes of gut microbial compositions, together with great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice.
32710966	6	75	theme	fatty	1529:1533	arg1	acids					1535:1539	fecal short chain fatty acids	1511:1539	fecal short chain fatty acids (SCFAs)	1511:1547	Further studies indicated PM treatment resulted in changes of gut microbial compositions, together with great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice.
32710966	1	76	theme	neuroprotective	162:176	arg1	effects					178:184	potentially neuroprotective effects	150:184	potentially neuroprotective effects	150:184	The study aims to investigate the potentially neuroprotective effects and underlying mechanisms for brown seaweed polysaccharide of polymannuronic acid (PM) against Parkinson's disease (PD) pathogenesis.
32710966	5	77	theme	junction	1242:1249	arg1	proteins					1262:1269	tight junction associated proteins	1236:1269	tight junction associated proteins in both mice colon and SNpc	1236:1297	Meanwhile, PM greatly improved integrity of intestinal barrier and blood brain barrier (BBB) as indicated by increased expressions of tight junction associated proteins in both mice colon and SNpc.
32710966	3	78	theme	acid	825:828	arg1	contents					705:712	contents	705:712	contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA)	705:835	The results showed PM administration improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc) and enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice.
32710966	4	79	theme	systemic	908:915	arg1	circulation					917:927	systemic circulation	908:927	systemic circulation	908:927	PM significantly alleviated inflammation in gut, brain and systemic circulation as shown by reduced levels or expressions of pro-inflammatory cytokines concurrently and inhibited mitogen-activated protein kinases (MAPK) signaling pathway in mice colon.
32710966	8	80	theme	gut	1869:1871	arg1	microbiota					1873:1882	gut microbiota	1869:1882	gut microbiota	1869:1882	PM might modulate brain-gut-microbiota axis, at least in part, via gut microbiota derived SCFAs as mediators.
32710966	7	81	theme	PD	1660:1661	arg1	pathogenesis					1663:1674	PD pathogenesis	1660:1674	PD pathogenesis	1660:1674	In conclusion, pre-administration of PM could provide neuroprotective effects against PD pathogenesis by suppressing inflammation in gut, brain and systemic circulation, and by improving integrity of intestinal barrier and BBB.
32710966	6	82	theme	mice	1568:1571	arg1	colon					1556:1560	the colon	1552:1560	the colon of PD mice	1552:1571	Further studies indicated PM treatment resulted in changes of gut microbial compositions, together with great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice.
32710966	2	83	theme	PM	431:432	arg1	effects					420:426	the preventative effects	403:426	the preventative effects of PM against neuronal loss together with its modulation on brain-gut-microbiota axis	403:512	PD model mice were pretreated with PM via oral gavage once per day for 4 weeks and the preventative effects of PM against neuronal loss together with its modulation on brain-gut-microbiota axis were systematically explored.
32710966	1	84	dep	effects	178:184	arg1	the					146:148	the	146:148	the	146:148	The study aims to investigate the potentially neuroprotective effects and underlying mechanisms for brown seaweed polysaccharide of polymannuronic acid (PM) against Parkinson's disease (PD) pathogenesis.
32710966	0	85	theme	dopaminergic	29:40	arg1	loss					51:54	dopaminergic neuronal loss	29:54	dopaminergic neuronal loss	29:54	Polymannuronic acid prevents dopaminergic neuronal loss via brain-gut-microbiota axis in Parkinson's disease model.
32710966	5	86	theme	blood	1169:1173	arg1	BBB					1190:1192	BBB	1190:1192	BBB	1190:1192	Meanwhile, PM greatly improved integrity of intestinal barrier and blood brain barrier (BBB) as indicated by increased expressions of tight junction associated proteins in both mice colon and SNpc.
32710966	5	86	theme	blood	1169:1173	arg1	barrier					1181:1187	blood brain barrier	1169:1187	blood brain barrier (BBB)	1169:1193	Meanwhile, PM greatly improved integrity of intestinal barrier and blood brain barrier (BBB) as indicated by increased expressions of tight junction associated proteins in both mice colon and SNpc.
32710966	6	87	dep	together	1390:1397	arg1	with					1399:1402	with	1399:1402	with	1399:1402	Further studies indicated PM treatment resulted in changes of gut microbial compositions, together with great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice.
32710966	6	88	from	increase	1499:1506	arg1	colon					1556:1560	the colon	1552:1560	the colon of PD mice	1552:1571	Further studies indicated PM treatment resulted in changes of gut microbial compositions, together with great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice.
32710966	8	89	dep	derived	1884:1890	arg1	microbiota					1873:1882	gut microbiota	1869:1882	gut microbiota	1869:1882	PM might modulate brain-gut-microbiota axis, at least in part, via gut microbiota derived SCFAs as mediators.
32710966	3	90	theme	pars	671:674	arg1	SNpc					686:689	SNpc	686:689	SNpc	686:689	The results showed PM administration improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc) and enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice.
32710966	3	90	theme	pars	671:674	arg1	compacta					676:683	the substantia nigra pars compacta	650:683	the substantia nigra pars compacta (SNpc)	650:690	The results showed PM administration improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc) and enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice.
32710966	6	91	theme	great	1404:1408	arg1	alterations					1410:1420	great alterations	1404:1420	great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice	1404:1571	Further studies indicated PM treatment resulted in changes of gut microbial compositions, together with great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice.
32710966	5	92	theme	barrier	1181:1187	arg1	integrity					1133:1141	integrity	1133:1141	integrity of intestinal barrier and blood brain barrier (BBB)	1133:1193	Meanwhile, PM greatly improved integrity of intestinal barrier and blood brain barrier (BBB) as indicated by increased expressions of tight junction associated proteins in both mice colon and SNpc.
32710966	7	93	theme	PM	1611:1612	arg1	pre-administration					1589:1606	pre-administration	1589:1606	pre-administration of PM	1589:1612	In conclusion, pre-administration of PM could provide neuroprotective effects against PD pathogenesis by suppressing inflammation in gut, brain and systemic circulation, and by improving integrity of intestinal barrier and BBB.
32710966	3	94	theme	substantia	654:663	arg1	SNpc					686:689	SNpc	686:689	SNpc	686:689	The results showed PM administration improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc) and enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice.
32710966	3	94	theme	substantia	654:663	arg1	compacta					676:683	the substantia nigra pars compacta	650:683	the substantia nigra pars compacta (SNpc)	650:690	The results showed PM administration improved motor functions by preventing dopaminergic neuronal loss in the substantia nigra pars compacta (SNpc) and enhanced contents of striatal homovanillic acid (HVA), serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) and γ-aminobutyric acid (GABA) in PD mice.
32710966	0	95	theme	brain-gut-microbiota	60:79	arg1	axis					81:84	brain-gut-microbiota axis	60:84	brain-gut-microbiota axis in Parkinson's disease model	60:113	Polymannuronic acid prevents dopaminergic neuronal loss via brain-gut-microbiota axis in Parkinson's disease model.
32710966	4	96	theme	pro-inflammatory	974:989	arg1	cytokines					991:999	pro-inflammatory cytokines	974:999	pro-inflammatory cytokines	974:999	PM significantly alleviated inflammation in gut, brain and systemic circulation as shown by reduced levels or expressions of pro-inflammatory cytokines concurrently and inhibited mitogen-activated protein kinases (MAPK) signaling pathway in mice colon.
32710966	5	97	theme	barrier	1157:1163	arg1	integrity					1133:1141	integrity	1133:1141	integrity of intestinal barrier and blood brain barrier (BBB)	1133:1193	Meanwhile, PM greatly improved integrity of intestinal barrier and blood brain barrier (BBB) as indicated by increased expressions of tight junction associated proteins in both mice colon and SNpc.
32710966	6	98	theme	fats	1474:1477	arg1	metabolism					1439:1448	metabolism	1439:1448	metabolism	1439:1448	Further studies indicated PM treatment resulted in changes of gut microbial compositions, together with great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice.
32710966	6	98	theme	fats	1474:1477	arg1	digestion					1425:1433	digestion	1425:1433	digestion	1425:1433	Further studies indicated PM treatment resulted in changes of gut microbial compositions, together with great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice.
32710966	1	99	theme	acid	263:266	arg1	mechanisms					201:210	underlying mechanisms	190:210	underlying mechanisms for brown seaweed polysaccharide	190:243	The study aims to investigate the potentially neuroprotective effects and underlying mechanisms for brown seaweed polysaccharide of polymannuronic acid (PM) against Parkinson's disease (PD) pathogenesis.
32710966	1	99	theme	acid	263:266	arg1	effects					178:184	potentially neuroprotective effects	150:184	potentially neuroprotective effects	150:184	The study aims to investigate the potentially neuroprotective effects and underlying mechanisms for brown seaweed polysaccharide of polymannuronic acid (PM) against Parkinson's disease (PD) pathogenesis.
32710966	2	100	theme	oral	362:365	arg1	gavage					367:372	oral gavage	362:372	oral gavage once per day for 4 weeks	362:397	PD model mice were pretreated with PM via oral gavage once per day for 4 weeks and the preventative effects of PM against neuronal loss together with its modulation on brain-gut-microbiota axis were systematically explored.
32710966	6	101	theme	proteins	1461:1468	arg1	metabolism					1439:1448	metabolism	1439:1448	metabolism	1439:1448	Further studies indicated PM treatment resulted in changes of gut microbial compositions, together with great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice.
32710966	6	101	theme	proteins	1461:1468	arg1	digestion					1425:1433	digestion	1425:1433	digestion	1425:1433	Further studies indicated PM treatment resulted in changes of gut microbial compositions, together with great alterations of digestion and metabolism of dietary proteins and fats, which led to surge increase of fecal short chain fatty acids (SCFAs) in the colon of PD mice.
33203008	2	0	from	effect	363:368	arg1	sensations					420:429	appetite sensations	411:429	appetite sensations	411:429	We, therefore, aimed to investigate the effect of daily intake of FiberBind and RG-I on appetite sensations and fecal fat excretion.
33203008	2	0	from	effect	363:368	arg1	excretion					445:453	fecal fat excretion	435:453	fecal fat excretion	435:453	We, therefore, aimed to investigate the effect of daily intake of FiberBind and RG-I on appetite sensations and fecal fat excretion.
33203008	9	1	theme	peak	1384:1387	arg1	<					1419:1419	p < 0.05	1417:1424	p < 0.05	1417:1424	Compared to the control, RG-I and FiberBind lowered peak insulin concentration (both p < 0.05) and delayed time to peak for glucose (both p < 0.05).
33203008	9	1	theme	peak	1384:1387	arg1	concentration					1397:1409	peak insulin concentration	1384:1409	peak insulin concentration (both p < 0.05)	1384:1425	Compared to the control, RG-I and FiberBind lowered peak insulin concentration (both p < 0.05) and delayed time to peak for glucose (both p < 0.05).
33203008	8	2	theme	RG-I	1163:1166	arg1	fiber					1168:1172	The RG-I fiber	1159:1172	The RG-I fiber	1159:1172	The RG-I fiber caused higher postprandial glucose concentration compared to FiberBind (p < 0.05) and higher insulin concentration at 180 min compared to control (p < 0.05).
33203008	0	3	theme	Crossover	155:163	arg1	Trial					165:169	A Randomized Controlled Single-Blind Crossover Trial	118:169	A Randomized Controlled Single-Blind Crossover Trial	118:169	Potato Fibers Have Positive Effects on Subjective Appetite Sensations in Healthy Men, but Not on Fecal Fat Excretion: A Randomized Controlled Single-Blind Crossover Trial.
33203008	6	4	theme	%	1055:1055	arg1	fullness					1044:1051	fullness	1044:1051	fullness (9% ± 3%)	1044:1061	FiberBind also caused higher satiety (6% ± 2%) and fullness (9% ± 3%) compared to RG-I (all p < 0.01).
33203008	6	4	theme	%	1055:1055	arg1	%					1060:1060	9% ± 3%	1054:1060	9% ± 3%	1054:1060	FiberBind also caused higher satiety (6% ± 2%) and fullness (9% ± 3%) compared to RG-I (all p < 0.01).
33203008	5	5	theme	lower	935:939	arg1	desire					941:946	lower desire	935:946	lower desire to eat (7% ± 3% and 6% ± 3%) (all p < 0.05)	935:990	Compared to RG-I and control, FiberBind caused a higher composite satiety score (6% ± 2% and 5% ± 2%), lower prospective food consumption (5% ± 2% and 6% ± 2%), and lower desire to eat (7% ± 3% and 6% ± 3%) (all p < 0.05).
33203008	9	6	dep	peak	1447:1450	arg1	to					1444:1445	to	1444:1445	to	1444:1445	Compared to the control, RG-I and FiberBind lowered peak insulin concentration (both p < 0.05) and delayed time to peak for glucose (both p < 0.05).
33203008	4	7	theme	ad	712:713	arg1	intake					730:735	ad libitum energy intake	712:735	ad libitum energy intake	712:735	Appetite sensation and blood samples during a 3 h meal test, fecal fat content, and ad libitum energy intake were assessed after each period.
33203008	0	8	contain	Have	14:17	arg1	Fibers					7:12	Potato Fibers	0:12	Potato Fibers	0:12	Potato Fibers Have Positive Effects on Subjective Appetite Sensations in Healthy Men, but Not on Fecal Fat Excretion: A Randomized Controlled Single-Blind Crossover Trial.
33203008	0	8	contain	Have	14:17	arg2	Effects					28:34	Positive Effects	19:34	Positive Effects on Subjective Appetite Sensations in Healthy Men, but Not on Fecal Fat Excretion	19:115	Potato Fibers Have Positive Effects on Subjective Appetite Sensations in Healthy Men, but Not on Fecal Fat Excretion: A Randomized Controlled Single-Blind Crossover Trial.
33203008	4	9	theme	energy	723:728	arg1	intake					730:735	ad libitum energy intake	712:735	ad libitum energy intake	712:735	Appetite sensation and blood samples during a 3 h meal test, fecal fat content, and ad libitum energy intake were assessed after each period.
33203008	6	10	theme	p	1085:1085	arg1	RG-I					1075:1078	RG-I	1075:1078	RG-I (all p < 0.01)	1075:1093	FiberBind also caused higher satiety (6% ± 2%) and fullness (9% ± 3%) compared to RG-I (all p < 0.01).
33203008	6	10	theme	p	1085:1085	arg1	<					1087:1087	all p < 0.01	1081:1092	all p < 0.01	1081:1092	FiberBind also caused higher satiety (6% ± 2%) and fullness (9% ± 3%) compared to RG-I (all p < 0.01).
33203008	1	11	theme	Dietary	172:178	arg1	fibers					180:185	Dietary fibers	172:185	Dietary fibers	172:185	Dietary fibers can affect appetite and gut metabolism, but the effect of the novel potato fibers FiberBind and rhamnogalacturonan I (RG-I) is unknown.
33203008	10	12	theme	fecal	1598:1602	arg1	excretion					1608:1616	fecal fat excretion	1598:1616	fecal fat excretion	1598:1616	In conclusion, FiberBind intake could be beneficial for appetite regulation, but neither FiberBind nor RG-I affected fecal fat excretion or energy intake.
33203008	0	13	theme	Fecal	97:101	arg1	Excretion					107:115	Fecal Fat Excretion	97:115	Fecal Fat Excretion	97:115	Potato Fibers Have Positive Effects on Subjective Appetite Sensations in Healthy Men, but Not on Fecal Fat Excretion: A Randomized Controlled Single-Blind Crossover Trial.
33203008	3	14	theme	crossover	499:507	arg1	trial					509:513	a single-blinded, randomized, three-way crossover trial	459:513	a single-blinded, randomized, three-way crossover trial	459:513	In a single-blinded, randomized, three-way crossover trial, wheat buns with FiberBind, RG-I, or low fiber (control) were consumed by 18 healthy men during a 21-day period.
33203008	10	15	theme	appetite	1537:1544	arg1	regulation					1546:1555	appetite regulation	1537:1555	appetite regulation	1537:1555	In conclusion, FiberBind intake could be beneficial for appetite regulation, but neither FiberBind nor RG-I affected fecal fat excretion or energy intake.
33203008	0	16	from	Sensations	59:68	arg1	Men					81:83	Healthy Men	73:83	Healthy Men	73:83	Potato Fibers Have Positive Effects on Subjective Appetite Sensations in Healthy Men, but Not on Fecal Fat Excretion: A Randomized Controlled Single-Blind Crossover Trial.
33203008	10	17	theme	energy	1621:1626	arg1	intake					1628:1633	energy intake	1621:1633	energy intake	1621:1633	In conclusion, FiberBind intake could be beneficial for appetite regulation, but neither FiberBind nor RG-I affected fecal fat excretion or energy intake.
33203008	5	18	theme	%	864:864	arg1	%					869:869	6% ± 2% and 5% ± 2%	851:869	%	869:869	Compared to RG-I and control, FiberBind caused a higher composite satiety score (6% ± 2% and 5% ± 2%), lower prospective food consumption (5% ± 2% and 6% ± 2%), and lower desire to eat (7% ± 3% and 6% ± 3%) (all p < 0.05).
33203008	5	19	theme	%	969:969	arg1	%					974:974	7% ± 3% and 6% ± 3%	956:974	%	974:974	Compared to RG-I and control, FiberBind caused a higher composite satiety score (6% ± 2% and 5% ± 2%), lower prospective food consumption (5% ± 2% and 6% ± 2%), and lower desire to eat (7% ± 3% and 6% ± 3%) (all p < 0.05).
33203008	0	20	theme	Controlled	131:140	arg1	Trial					165:169	A Randomized Controlled Single-Blind Crossover Trial	118:169	A Randomized Controlled Single-Blind Crossover Trial	118:169	Potato Fibers Have Positive Effects on Subjective Appetite Sensations in Healthy Men, but Not on Fecal Fat Excretion: A Randomized Controlled Single-Blind Crossover Trial.
33203008	7	21	theme	energy	1133:1138	arg1	intake					1140:1145	energy intake	1133:1145	energy intake	1133:1145	No effects on fecal fat excretion or energy intake were found.
33203008	2	22	theme	fat	441:443	arg1	excretion					445:453	fecal fat excretion	435:453	fecal fat excretion	435:453	We, therefore, aimed to investigate the effect of daily intake of FiberBind and RG-I on appetite sensations and fecal fat excretion.
33203008	6	23	theme	higher	1015:1020	arg1	satiety					1022:1028	higher satiety	1015:1028	higher satiety (6% ± 2%)	1015:1038	FiberBind also caused higher satiety (6% ± 2%) and fullness (9% ± 3%) compared to RG-I (all p < 0.01).
33203008	6	23	theme	higher	1015:1020	arg1	%					1037:1037	6% ± 2%	1031:1037	6% ± 2%	1031:1037	FiberBind also caused higher satiety (6% ± 2%) and fullness (9% ± 3%) compared to RG-I (all p < 0.01).
33203008	5	24	theme	%	910:910	arg1	%					915:915	5% ± 2% and 6% ± 2%	909:927	%	915:915	Compared to RG-I and control, FiberBind caused a higher composite satiety score (6% ± 2% and 5% ± 2%), lower prospective food consumption (5% ± 2% and 6% ± 2%), and lower desire to eat (7% ± 3% and 6% ± 3%) (all p < 0.05).
33203008	8	25	theme	p	1321:1321	arg1	control					1312:1318	control	1312:1318	control (p < 0.05)	1312:1329	The RG-I fiber caused higher postprandial glucose concentration compared to FiberBind (p < 0.05) and higher insulin concentration at 180 min compared to control (p < 0.05).
33203008	8	25	theme	p	1321:1321	arg1	<					1323:1323	p < 0.05	1321:1328	p < 0.05	1321:1328	The RG-I fiber caused higher postprandial glucose concentration compared to FiberBind (p < 0.05) and higher insulin concentration at 180 min compared to control (p < 0.05).
33203008	1	26	dep	fibers	262:267	arg1	fibers					262:267	the novel potato fibers	245:267	the novel potato fibers FiberBind and rhamnogalacturonan I (RG-I)	245:309	Dietary fibers can affect appetite and gut metabolism, but the effect of the novel potato fibers FiberBind and rhamnogalacturonan I (RG-I) is unknown.
33203008	1	26	dep	fibers	262:267	arg1	RG-I					305:308	RG-I	305:308	RG-I	305:308	Dietary fibers can affect appetite and gut metabolism, but the effect of the novel potato fibers FiberBind and rhamnogalacturonan I (RG-I) is unknown.
33203008	1	26	dep	fibers	262:267	arg1	FiberBind					269:277	FiberBind	269:277	FiberBind	269:277	Dietary fibers can affect appetite and gut metabolism, but the effect of the novel potato fibers FiberBind and rhamnogalacturonan I (RG-I) is unknown.
33203008	1	26	dep	fibers	262:267	arg1	I					302:302	rhamnogalacturonan I	283:302	rhamnogalacturonan I (RG-I)	283:309	Dietary fibers can affect appetite and gut metabolism, but the effect of the novel potato fibers FiberBind and rhamnogalacturonan I (RG-I) is unknown.
33203008	9	27	theme	p	1470:1470	arg1	peak					1447:1450	time to peak	1439:1450	time to peak for glucose (both p < 0.05)	1439:1478	Compared to the control, RG-I and FiberBind lowered peak insulin concentration (both p < 0.05) and delayed time to peak for glucose (both p < 0.05).
33203008	9	27	theme	p	1470:1470	arg1	<					1472:1472	p < 0.05	1470:1477	p < 0.05	1470:1477	Compared to the control, RG-I and FiberBind lowered peak insulin concentration (both p < 0.05) and delayed time to peak for glucose (both p < 0.05).
33203008	10	28	theme	FiberBind	1496:1504	arg1	intake					1506:1511	FiberBind intake	1496:1511	FiberBind intake	1496:1511	In conclusion, FiberBind intake could be beneficial for appetite regulation, but neither FiberBind nor RG-I affected fecal fat excretion or energy intake.
33203008	0	29	theme	Healthy	73:79	arg1	Men					81:83	Healthy Men	73:83	Healthy Men	73:83	Potato Fibers Have Positive Effects on Subjective Appetite Sensations in Healthy Men, but Not on Fecal Fat Excretion: A Randomized Controlled Single-Blind Crossover Trial.
33203008	7	30	theme	fecal	1110:1114	arg1	excretion					1120:1128	fecal fat excretion	1110:1128	fecal fat excretion	1110:1128	No effects on fecal fat excretion or energy intake were found.
33203008	3	31	with	buns	522:525	arg1	RG-I					543:546	RG-I	543:546	RG-I	543:546	In a single-blinded, randomized, three-way crossover trial, wheat buns with FiberBind, RG-I, or low fiber (control) were consumed by 18 healthy men during a 21-day period.
33203008	3	31	with	buns	522:525	arg1	control					563:569	control	563:569	control	563:569	In a single-blinded, randomized, three-way crossover trial, wheat buns with FiberBind, RG-I, or low fiber (control) were consumed by 18 healthy men during a 21-day period.
33203008	3	31	with	buns	522:525	arg1	FiberBind					532:540	FiberBind	532:540	FiberBind	532:540	In a single-blinded, randomized, three-way crossover trial, wheat buns with FiberBind, RG-I, or low fiber (control) were consumed by 18 healthy men during a 21-day period.
33203008	3	31	with	buns	522:525	arg1	fiber					556:560	low fiber	552:560	low fiber (control)	552:570	In a single-blinded, randomized, three-way crossover trial, wheat buns with FiberBind, RG-I, or low fiber (control) were consumed by 18 healthy men during a 21-day period.
33203008	5	32	theme	food	891:894	arg1	consumption					896:906	lower prospective food consumption	873:906	lower prospective food consumption (5% ± 2% and 6% ± 2%)	873:928	Compared to RG-I and control, FiberBind caused a higher composite satiety score (6% ± 2% and 5% ± 2%), lower prospective food consumption (5% ± 2% and 6% ± 2%), and lower desire to eat (7% ± 3% and 6% ± 3%) (all p < 0.05).
33203008	5	33	theme	composite	826:834	arg1	score					844:848	a higher composite satiety score	817:848	a higher composite satiety score (6% ± 2% and 5% ± 2%)	817:870	Compared to RG-I and control, FiberBind caused a higher composite satiety score (6% ± 2% and 5% ± 2%), lower prospective food consumption (5% ± 2% and 6% ± 2%), and lower desire to eat (7% ± 3% and 6% ± 3%) (all p < 0.05).
33203008	4	34	theme	3	674:674	arg1	h					676:676	h	676:676	h	676:676	Appetite sensation and blood samples during a 3 h meal test, fecal fat content, and ad libitum energy intake were assessed after each period.
33203008	2	35	theme	FiberBind	389:397	arg1	intake					379:384	daily intake	373:384	daily intake of FiberBind and RG-I	373:406	We, therefore, aimed to investigate the effect of daily intake of FiberBind and RG-I on appetite sensations and fecal fat excretion.
33203008	0	36	from	Effects	28:34	arg1	Excretion					107:115	Fecal Fat Excretion	97:115	Fecal Fat Excretion	97:115	Potato Fibers Have Positive Effects on Subjective Appetite Sensations in Healthy Men, but Not on Fecal Fat Excretion: A Randomized Controlled Single-Blind Crossover Trial.
33203008	0	36	from	Effects	28:34	arg1	Sensations					59:68	Subjective Appetite Sensations	39:68	Subjective Appetite Sensations in Healthy Men	39:83	Potato Fibers Have Positive Effects on Subjective Appetite Sensations in Healthy Men, but Not on Fecal Fat Excretion: A Randomized Controlled Single-Blind Crossover Trial.
33203008	0	37	theme	Potato	0:5	arg1	Fibers					7:12	Potato Fibers	0:12	Potato Fibers	0:12	Potato Fibers Have Positive Effects on Subjective Appetite Sensations in Healthy Men, but Not on Fecal Fat Excretion: A Randomized Controlled Single-Blind Crossover Trial.
33203008	2	38	theme	intake	379:384	arg1	effect					363:368	the effect	359:368	the effect of daily intake of FiberBind and RG-I on appetite sensations and fecal fat excretion	359:453	We, therefore, aimed to investigate the effect of daily intake of FiberBind and RG-I on appetite sensations and fecal fat excretion.
33203008	4	39	theme	blood	651:655	arg1	samples					657:663	Appetite sensation and blood samples	628:663	samples	657:663	Appetite sensation and blood samples during a 3 h meal test, fecal fat content, and ad libitum energy intake were assessed after each period.
33203008	1	40	theme	potato	255:260	arg1	fibers					262:267	the novel potato fibers	245:267	the novel potato fibers FiberBind and rhamnogalacturonan I (RG-I)	245:309	Dietary fibers can affect appetite and gut metabolism, but the effect of the novel potato fibers FiberBind and rhamnogalacturonan I (RG-I) is unknown.
33203008	1	40	theme	potato	255:260	arg1	FiberBind					269:277	FiberBind	269:277	FiberBind	269:277	Dietary fibers can affect appetite and gut metabolism, but the effect of the novel potato fibers FiberBind and rhamnogalacturonan I (RG-I) is unknown.
33203008	1	40	theme	potato	255:260	arg1	I					302:302	rhamnogalacturonan I	283:302	rhamnogalacturonan I (RG-I)	283:309	Dietary fibers can affect appetite and gut metabolism, but the effect of the novel potato fibers FiberBind and rhamnogalacturonan I (RG-I) is unknown.
33203008	7	41	from	effects	1099:1105	arg1	excretion					1120:1128	fecal fat excretion	1110:1128	fecal fat excretion	1110:1128	No effects on fecal fat excretion or energy intake were found.
33203008	7	41	from	effects	1099:1105	arg1	intake					1140:1145	energy intake	1133:1145	energy intake	1133:1145	No effects on fecal fat excretion or energy intake were found.
33203008	2	42	theme	appetite	411:418	arg1	sensations					420:429	appetite sensations	411:429	appetite sensations	411:429	We, therefore, aimed to investigate the effect of daily intake of FiberBind and RG-I on appetite sensations and fecal fat excretion.
33203008	4	43	theme	fat	695:697	arg1	content					699:705	fecal fat content	689:705	fecal fat content	689:705	Appetite sensation and blood samples during a 3 h meal test, fecal fat content, and ad libitum energy intake were assessed after each period.
33203008	2	44	theme	RG-I	403:406	arg1	intake					379:384	daily intake	373:384	daily intake of FiberBind and RG-I	373:406	We, therefore, aimed to investigate the effect of daily intake of FiberBind and RG-I on appetite sensations and fecal fat excretion.
33203008	6	45	theme	%	1032:1032	arg1	satiety					1022:1028	higher satiety	1015:1028	higher satiety (6% ± 2%)	1015:1038	FiberBind also caused higher satiety (6% ± 2%) and fullness (9% ± 3%) compared to RG-I (all p < 0.01).
33203008	6	45	theme	%	1032:1032	arg1	%					1037:1037	6% ± 2%	1031:1037	6% ± 2%	1031:1037	FiberBind also caused higher satiety (6% ± 2%) and fullness (9% ± 3%) compared to RG-I (all p < 0.01).
33203008	5	46	theme	%	922:922	arg1	%					927:927	5% ± 2% and 6% ± 2%	909:927	%	927:927	Compared to RG-I and control, FiberBind caused a higher composite satiety score (6% ± 2% and 5% ± 2%), lower prospective food consumption (5% ± 2% and 6% ± 2%), and lower desire to eat (7% ± 3% and 6% ± 3%) (all p < 0.05).
33203008	5	47	dep	consumption	896:906	arg1	%					927:927	5% ± 2% and 6% ± 2%	909:927	%	927:927	Compared to RG-I and control, FiberBind caused a higher composite satiety score (6% ± 2% and 5% ± 2%), lower prospective food consumption (5% ± 2% and 6% ± 2%), and lower desire to eat (7% ± 3% and 6% ± 3%) (all p < 0.05).
33203008	5	47	dep	consumption	896:906	arg1	%					915:915	5% ± 2% and 6% ± 2%	909:927	%	915:915	Compared to RG-I and control, FiberBind caused a higher composite satiety score (6% ± 2% and 5% ± 2%), lower prospective food consumption (5% ± 2% and 6% ± 2%), and lower desire to eat (7% ± 3% and 6% ± 3%) (all p < 0.05).
33203008	4	48	theme	meal	678:681	arg1	test					683:686	a 3 h meal test	672:686	a 3 h meal test	672:686	Appetite sensation and blood samples during a 3 h meal test, fecal fat content, and ad libitum energy intake were assessed after each period.
33203008	8	49	theme	higher	1181:1186	arg1	concentration					1209:1221	higher postprandial glucose concentration	1181:1221	higher postprandial glucose concentration	1181:1221	The RG-I fiber caused higher postprandial glucose concentration compared to FiberBind (p < 0.05) and higher insulin concentration at 180 min compared to control (p < 0.05).
33203008	0	50	dep	Have	14:17	arg1	Trial					165:169	A Randomized Controlled Single-Blind Crossover Trial	118:169	A Randomized Controlled Single-Blind Crossover Trial	118:169	Potato Fibers Have Positive Effects on Subjective Appetite Sensations in Healthy Men, but Not on Fecal Fat Excretion: A Randomized Controlled Single-Blind Crossover Trial.
33203008	8	51	theme	glucose	1201:1207	arg1	concentration					1209:1221	higher postprandial glucose concentration	1181:1221	higher postprandial glucose concentration	1181:1221	The RG-I fiber caused higher postprandial glucose concentration compared to FiberBind (p < 0.05) and higher insulin concentration at 180 min compared to control (p < 0.05).
33203008	0	52	theme	Appetite	50:57	arg1	Sensations					59:68	Subjective Appetite Sensations	39:68	Subjective Appetite Sensations in Healthy Men	39:83	Potato Fibers Have Positive Effects on Subjective Appetite Sensations in Healthy Men, but Not on Fecal Fat Excretion: A Randomized Controlled Single-Blind Crossover Trial.
33203008	5	53	theme	±	854:854	arg1	%					857:857	6% ± 2% and 5% ± 2%	851:869	%	857:857	Compared to RG-I and control, FiberBind caused a higher composite satiety score (6% ± 2% and 5% ± 2%), lower prospective food consumption (5% ± 2% and 6% ± 2%), and lower desire to eat (7% ± 3% and 6% ± 3%) (all p < 0.05).
33203008	6	54	theme	±	1057:1057	arg1	fullness					1044:1051	fullness	1044:1051	fullness (9% ± 3%)	1044:1061	FiberBind also caused higher satiety (6% ± 2%) and fullness (9% ± 3%) compared to RG-I (all p < 0.01).
33203008	6	54	theme	±	1057:1057	arg1	%					1060:1060	9% ± 3%	1054:1060	9% ± 3%	1054:1060	FiberBind also caused higher satiety (6% ± 2%) and fullness (9% ± 3%) compared to RG-I (all p < 0.01).
33203008	9	55	theme	insulin	1389:1395	arg1	<					1419:1419	p < 0.05	1417:1424	p < 0.05	1417:1424	Compared to the control, RG-I and FiberBind lowered peak insulin concentration (both p < 0.05) and delayed time to peak for glucose (both p < 0.05).
33203008	9	55	theme	insulin	1389:1395	arg1	concentration					1397:1409	peak insulin concentration	1384:1409	peak insulin concentration (both p < 0.05)	1384:1425	Compared to the control, RG-I and FiberBind lowered peak insulin concentration (both p < 0.05) and delayed time to peak for glucose (both p < 0.05).
33203008	3	56	theme	healthy	592:598	arg1	men					600:602	18 healthy men	589:602	18 healthy men	589:602	In a single-blinded, randomized, three-way crossover trial, wheat buns with FiberBind, RG-I, or low fiber (control) were consumed by 18 healthy men during a 21-day period.
33203008	3	57	theme	single-blinded	461:474	arg1	trial					509:513	a single-blinded, randomized, three-way crossover trial	459:513	a single-blinded, randomized, three-way crossover trial	459:513	In a single-blinded, randomized, three-way crossover trial, wheat buns with FiberBind, RG-I, or low fiber (control) were consumed by 18 healthy men during a 21-day period.
33203008	8	58	theme	p	1246:1246	arg1	FiberBind					1235:1243	FiberBind	1235:1243	FiberBind (p < 0.05)	1235:1254	The RG-I fiber caused higher postprandial glucose concentration compared to FiberBind (p < 0.05) and higher insulin concentration at 180 min compared to control (p < 0.05).
33203008	8	58	theme	p	1246:1246	arg1	<					1248:1248	p < 0.05	1246:1253	p < 0.05	1246:1253	The RG-I fiber caused higher postprandial glucose concentration compared to FiberBind (p < 0.05) and higher insulin concentration at 180 min compared to control (p < 0.05).
33203008	0	59	theme	Subjective	39:48	arg1	Sensations					59:68	Subjective Appetite Sensations	39:68	Subjective Appetite Sensations in Healthy Men	39:83	Potato Fibers Have Positive Effects on Subjective Appetite Sensations in Healthy Men, but Not on Fecal Fat Excretion: A Randomized Controlled Single-Blind Crossover Trial.
33203008	9	60	theme	p	1417:1417	arg1	<					1419:1419	p < 0.05	1417:1424	p < 0.05	1417:1424	Compared to the control, RG-I and FiberBind lowered peak insulin concentration (both p < 0.05) and delayed time to peak for glucose (both p < 0.05).
33203008	9	60	theme	p	1417:1417	arg1	concentration					1397:1409	peak insulin concentration	1384:1409	peak insulin concentration (both p < 0.05)	1384:1425	Compared to the control, RG-I and FiberBind lowered peak insulin concentration (both p < 0.05) and delayed time to peak for glucose (both p < 0.05).
33203008	1	61	theme	rhamnogalacturonan	283:300	arg1	fibers					262:267	the novel potato fibers	245:267	the novel potato fibers FiberBind and rhamnogalacturonan I (RG-I)	245:309	Dietary fibers can affect appetite and gut metabolism, but the effect of the novel potato fibers FiberBind and rhamnogalacturonan I (RG-I) is unknown.
33203008	1	61	theme	rhamnogalacturonan	283:300	arg1	RG-I					305:308	RG-I	305:308	RG-I	305:308	Dietary fibers can affect appetite and gut metabolism, but the effect of the novel potato fibers FiberBind and rhamnogalacturonan I (RG-I) is unknown.
33203008	1	61	theme	rhamnogalacturonan	283:300	arg1	I					302:302	rhamnogalacturonan I	283:302	rhamnogalacturonan I (RG-I)	283:309	Dietary fibers can affect appetite and gut metabolism, but the effect of the novel potato fibers FiberBind and rhamnogalacturonan I (RG-I) is unknown.
33203008	3	62	theme	21-day	613:618	arg1	period					620:625	a 21-day period	611:625	a 21-day period	611:625	In a single-blinded, randomized, three-way crossover trial, wheat buns with FiberBind, RG-I, or low fiber (control) were consumed by 18 healthy men during a 21-day period.
33203008	4	63	theme	libitum	715:721	arg1	intake					730:735	ad libitum energy intake	712:735	ad libitum energy intake	712:735	Appetite sensation and blood samples during a 3 h meal test, fecal fat content, and ad libitum energy intake were assessed after each period.
33203008	10	64	theme	fat	1604:1606	arg1	excretion					1608:1616	fecal fat excretion	1598:1616	fecal fat excretion	1598:1616	In conclusion, FiberBind intake could be beneficial for appetite regulation, but neither FiberBind nor RG-I affected fecal fat excretion or energy intake.
33203008	0	65	theme	Fat	103:105	arg1	Excretion					107:115	Fecal Fat Excretion	97:115	Fecal Fat Excretion	97:115	Potato Fibers Have Positive Effects on Subjective Appetite Sensations in Healthy Men, but Not on Fecal Fat Excretion: A Randomized Controlled Single-Blind Crossover Trial.
33203008	8	66	theme	higher	1260:1265	arg1	concentration					1275:1287	higher insulin concentration	1260:1287	higher insulin concentration	1260:1287	The RG-I fiber caused higher postprandial glucose concentration compared to FiberBind (p < 0.05) and higher insulin concentration at 180 min compared to control (p < 0.05).
33203008	8	67	theme	insulin	1267:1273	arg1	concentration					1275:1287	higher insulin concentration	1260:1287	higher insulin concentration	1260:1287	The RG-I fiber caused higher postprandial glucose concentration compared to FiberBind (p < 0.05) and higher insulin concentration at 180 min compared to control (p < 0.05).
33203008	5	68	dep	eat	951:953	arg1	%					962:962	7% ± 3% and 6% ± 3%	956:974	%	962:962	Compared to RG-I and control, FiberBind caused a higher composite satiety score (6% ± 2% and 5% ± 2%), lower prospective food consumption (5% ± 2% and 6% ± 2%), and lower desire to eat (7% ± 3% and 6% ± 3%) (all p < 0.05).
33203008	5	68	dep	eat	951:953	arg1	%					974:974	7% ± 3% and 6% ± 3%	956:974	%	974:974	Compared to RG-I and control, FiberBind caused a higher composite satiety score (6% ± 2% and 5% ± 2%), lower prospective food consumption (5% ± 2% and 6% ± 2%), and lower desire to eat (7% ± 3% and 6% ± 3%) (all p < 0.05).
33203008	9	69	theme	time	1439:1442	arg1	peak					1447:1450	time to peak	1439:1450	time to peak for glucose (both p < 0.05)	1439:1478	Compared to the control, RG-I and FiberBind lowered peak insulin concentration (both p < 0.05) and delayed time to peak for glucose (both p < 0.05).
33203008	9	69	theme	time	1439:1442	arg1	<					1472:1472	p < 0.05	1470:1477	p < 0.05	1470:1477	Compared to the control, RG-I and FiberBind lowered peak insulin concentration (both p < 0.05) and delayed time to peak for glucose (both p < 0.05).
33203008	0	70	theme	Randomized	120:129	arg1	Trial					165:169	A Randomized Controlled Single-Blind Crossover Trial	118:169	A Randomized Controlled Single-Blind Crossover Trial	118:169	Potato Fibers Have Positive Effects on Subjective Appetite Sensations in Healthy Men, but Not on Fecal Fat Excretion: A Randomized Controlled Single-Blind Crossover Trial.
33203008	5	71	theme	prospective	879:889	arg1	consumption					896:906	lower prospective food consumption	873:906	lower prospective food consumption (5% ± 2% and 6% ± 2%)	873:928	Compared to RG-I and control, FiberBind caused a higher composite satiety score (6% ± 2% and 5% ± 2%), lower prospective food consumption (5% ± 2% and 6% ± 2%), and lower desire to eat (7% ± 3% and 6% ± 3%) (all p < 0.05).
33203008	3	72	theme	wheat	516:520	arg1	buns					522:525	wheat buns	516:525	wheat buns with FiberBind, RG-I, or low fiber (control)	516:570	In a single-blinded, randomized, three-way crossover trial, wheat buns with FiberBind, RG-I, or low fiber (control) were consumed by 18 healthy men during a 21-day period.
33203008	9	73	dep	<	1419:1419	arg1	both					1412:1415	both	1412:1415	both	1412:1415	Compared to the control, RG-I and FiberBind lowered peak insulin concentration (both p < 0.05) and delayed time to peak for glucose (both p < 0.05).
33203008	3	74	dep	single-blinded	461:474	arg1	randomized					477:486	randomized	477:486	randomized	477:486	In a single-blinded, randomized, three-way crossover trial, wheat buns with FiberBind, RG-I, or low fiber (control) were consumed by 18 healthy men during a 21-day period.
33203008	3	74	dep	single-blinded	461:474	arg1	three-way					489:497	three-way	489:497	three-way	489:497	In a single-blinded, randomized, three-way crossover trial, wheat buns with FiberBind, RG-I, or low fiber (control) were consumed by 18 healthy men during a 21-day period.
33203008	5	75	dep	score	844:848	arg1	%					857:857	6% ± 2% and 5% ± 2%	851:869	%	857:857	Compared to RG-I and control, FiberBind caused a higher composite satiety score (6% ± 2% and 5% ± 2%), lower prospective food consumption (5% ± 2% and 6% ± 2%), and lower desire to eat (7% ± 3% and 6% ± 3%) (all p < 0.05).
33203008	5	75	dep	score	844:848	arg1	%					869:869	6% ± 2% and 5% ± 2%	851:869	%	869:869	Compared to RG-I and control, FiberBind caused a higher composite satiety score (6% ± 2% and 5% ± 2%), lower prospective food consumption (5% ± 2% and 6% ± 2%), and lower desire to eat (7% ± 3% and 6% ± 3%) (all p < 0.05).
33203008	0	76	theme	Single-Blind	142:153	arg1	Trial					165:169	A Randomized Controlled Single-Blind Crossover Trial	118:169	A Randomized Controlled Single-Blind Crossover Trial	118:169	Potato Fibers Have Positive Effects on Subjective Appetite Sensations in Healthy Men, but Not on Fecal Fat Excretion: A Randomized Controlled Single-Blind Crossover Trial.
33203008	5	77	theme	±	866:866	arg1	%					869:869	6% ± 2% and 5% ± 2%	851:869	%	869:869	Compared to RG-I and control, FiberBind caused a higher composite satiety score (6% ± 2% and 5% ± 2%), lower prospective food consumption (5% ± 2% and 6% ± 2%), and lower desire to eat (7% ± 3% and 6% ± 3%) (all p < 0.05).
33203008	5	78	theme	±	971:971	arg1	%					974:974	7% ± 3% and 6% ± 3%	956:974	%	974:974	Compared to RG-I and control, FiberBind caused a higher composite satiety score (6% ± 2% and 5% ± 2%), lower prospective food consumption (5% ± 2% and 6% ± 2%), and lower desire to eat (7% ± 3% and 6% ± 3%) (all p < 0.05).
33203008	7	79	theme	fat	1116:1118	arg1	excretion					1120:1128	fecal fat excretion	1110:1128	fecal fat excretion	1110:1128	No effects on fecal fat excretion or energy intake were found.
33203008	2	80	theme	fecal	435:439	arg1	excretion					445:453	fecal fat excretion	435:453	fecal fat excretion	435:453	We, therefore, aimed to investigate the effect of daily intake of FiberBind and RG-I on appetite sensations and fecal fat excretion.
33203008	5	81	theme	±	959:959	arg1	%					962:962	7% ± 3% and 6% ± 3%	956:974	%	962:962	Compared to RG-I and control, FiberBind caused a higher composite satiety score (6% ± 2% and 5% ± 2%), lower prospective food consumption (5% ± 2% and 6% ± 2%), and lower desire to eat (7% ± 3% and 6% ± 3%) (all p < 0.05).
33203008	5	82	theme	±	912:912	arg1	%					915:915	5% ± 2% and 6% ± 2%	909:927	%	915:915	Compared to RG-I and control, FiberBind caused a higher composite satiety score (6% ± 2% and 5% ± 2%), lower prospective food consumption (5% ± 2% and 6% ± 2%), and lower desire to eat (7% ± 3% and 6% ± 3%) (all p < 0.05).
33203008	5	83	theme	higher	819:824	arg1	score					844:848	a higher composite satiety score	817:848	a higher composite satiety score (6% ± 2% and 5% ± 2%)	817:870	Compared to RG-I and control, FiberBind caused a higher composite satiety score (6% ± 2% and 5% ± 2%), lower prospective food consumption (5% ± 2% and 6% ± 2%), and lower desire to eat (7% ± 3% and 6% ± 3%) (all p < 0.05).
33203008	5	84	theme	p	982:982	arg1	<					984:984	all p < 0.05	978:989	all p < 0.05	978:989	Compared to RG-I and control, FiberBind caused a higher composite satiety score (6% ± 2% and 5% ± 2%), lower prospective food consumption (5% ± 2% and 6% ± 2%), and lower desire to eat (7% ± 3% and 6% ± 3%) (all p < 0.05).
33203008	5	84	theme	p	982:982	arg1	eat					951:953	eat	951:953	eat (7% ± 3% and 6% ± 3%) (all p < 0.05)	951:990	Compared to RG-I and control, FiberBind caused a higher composite satiety score (6% ± 2% and 5% ± 2%), lower prospective food consumption (5% ± 2% and 6% ± 2%), and lower desire to eat (7% ± 3% and 6% ± 3%) (all p < 0.05).
33203008	5	85	theme	satiety	836:842	arg1	score					844:848	a higher composite satiety score	817:848	a higher composite satiety score (6% ± 2% and 5% ± 2%)	817:870	Compared to RG-I and control, FiberBind caused a higher composite satiety score (6% ± 2% and 5% ± 2%), lower prospective food consumption (5% ± 2% and 6% ± 2%), and lower desire to eat (7% ± 3% and 6% ± 3%) (all p < 0.05).
33203008	4	86	theme	Appetite	628:635	arg1	sensation					637:645	Appetite sensation and blood samples	628:663	sensation	637:645	Appetite sensation and blood samples during a 3 h meal test, fecal fat content, and ad libitum energy intake were assessed after each period.
33203008	0	87	theme	Positive	19:26	arg1	Effects					28:34	Positive Effects	19:34	Positive Effects on Subjective Appetite Sensations in Healthy Men, but Not on Fecal Fat Excretion	19:115	Potato Fibers Have Positive Effects on Subjective Appetite Sensations in Healthy Men, but Not on Fecal Fat Excretion: A Randomized Controlled Single-Blind Crossover Trial.
33203008	1	88	theme	novel	249:253	arg1	fibers					262:267	the novel potato fibers	245:267	the novel potato fibers FiberBind and rhamnogalacturonan I (RG-I)	245:309	Dietary fibers can affect appetite and gut metabolism, but the effect of the novel potato fibers FiberBind and rhamnogalacturonan I (RG-I) is unknown.
33203008	1	88	theme	novel	249:253	arg1	FiberBind					269:277	FiberBind	269:277	FiberBind	269:277	Dietary fibers can affect appetite and gut metabolism, but the effect of the novel potato fibers FiberBind and rhamnogalacturonan I (RG-I) is unknown.
33203008	1	88	theme	novel	249:253	arg1	I					302:302	rhamnogalacturonan I	283:302	rhamnogalacturonan I (RG-I)	283:309	Dietary fibers can affect appetite and gut metabolism, but the effect of the novel potato fibers FiberBind and rhamnogalacturonan I (RG-I) is unknown.
33203008	2	89	theme	daily	373:377	arg1	intake					379:384	daily intake	373:384	daily intake of FiberBind and RG-I	373:406	We, therefore, aimed to investigate the effect of daily intake of FiberBind and RG-I on appetite sensations and fecal fat excretion.
33203008	10	90	from	beneficial	1522:1531	arg1	conclusion					1484:1493	conclusion	1484:1493	conclusion	1484:1493	In conclusion, FiberBind intake could be beneficial for appetite regulation, but neither FiberBind nor RG-I affected fecal fat excretion or energy intake.
33203008	1	91	theme	fibers	262:267	arg1	effect					235:240	the effect	231:240	the effect of the novel potato fibers FiberBind and rhamnogalacturonan I (RG-I)	231:309	Dietary fibers can affect appetite and gut metabolism, but the effect of the novel potato fibers FiberBind and rhamnogalacturonan I (RG-I) is unknown.
33203008	1	91	theme	fibers	262:267	arg1	unknown					314:320	unknown	314:320	unknown	314:320	Dietary fibers can affect appetite and gut metabolism, but the effect of the novel potato fibers FiberBind and rhamnogalacturonan I (RG-I) is unknown.
33203008	3	92	theme	low	552:554	arg1	control					563:569	control	563:569	control	563:569	In a single-blinded, randomized, three-way crossover trial, wheat buns with FiberBind, RG-I, or low fiber (control) were consumed by 18 healthy men during a 21-day period.
33203008	3	92	theme	low	552:554	arg1	fiber					556:560	low fiber	552:560	low fiber (control)	552:570	In a single-blinded, randomized, three-way crossover trial, wheat buns with FiberBind, RG-I, or low fiber (control) were consumed by 18 healthy men during a 21-day period.
33203008	4	93	theme	fecal	689:693	arg1	content					699:705	fecal fat content	689:705	fecal fat content	689:705	Appetite sensation and blood samples during a 3 h meal test, fecal fat content, and ad libitum energy intake were assessed after each period.
33203008	6	94	theme	±	1034:1034	arg1	satiety					1022:1028	higher satiety	1015:1028	higher satiety (6% ± 2%)	1015:1038	FiberBind also caused higher satiety (6% ± 2%) and fullness (9% ± 3%) compared to RG-I (all p < 0.01).
33203008	6	94	theme	±	1034:1034	arg1	%					1037:1037	6% ± 2%	1031:1037	6% ± 2%	1031:1037	FiberBind also caused higher satiety (6% ± 2%) and fullness (9% ± 3%) compared to RG-I (all p < 0.01).
33203008	10	95	from	conclusion	1484:1493	arg1	beneficial					1522:1531	beneficial	1522:1531	beneficial	1522:1531	In conclusion, FiberBind intake could be beneficial for appetite regulation, but neither FiberBind nor RG-I affected fecal fat excretion or energy intake.
33203008	8	96	theme	postprandial	1188:1199	arg1	concentration					1209:1221	higher postprandial glucose concentration	1181:1221	higher postprandial glucose concentration	1181:1221	The RG-I fiber caused higher postprandial glucose concentration compared to FiberBind (p < 0.05) and higher insulin concentration at 180 min compared to control (p < 0.05).
33203008	5	97	theme	±	924:924	arg1	%					927:927	5% ± 2% and 6% ± 2%	909:927	%	927:927	Compared to RG-I and control, FiberBind caused a higher composite satiety score (6% ± 2% and 5% ± 2%), lower prospective food consumption (5% ± 2% and 6% ± 2%), and lower desire to eat (7% ± 3% and 6% ± 3%) (all p < 0.05).
33203008	4	98	theme	h	676:676	arg1	test					683:686	a 3 h meal test	672:686	a 3 h meal test	672:686	Appetite sensation and blood samples during a 3 h meal test, fecal fat content, and ad libitum energy intake were assessed after each period.
33203008	9	99	dep	<	1472:1472	arg1	both					1465:1468	both	1465:1468	both	1465:1468	Compared to the control, RG-I and FiberBind lowered peak insulin concentration (both p < 0.05) and delayed time to peak for glucose (both p < 0.05).
33203008	1	100	theme	gut	211:213	arg1	metabolism					215:224	gut metabolism	211:224	gut metabolism	211:224	Dietary fibers can affect appetite and gut metabolism, but the effect of the novel potato fibers FiberBind and rhamnogalacturonan I (RG-I) is unknown.
33203008	5	101	theme	%	852:852	arg1	%					857:857	6% ± 2% and 5% ± 2%	851:869	%	857:857	Compared to RG-I and control, FiberBind caused a higher composite satiety score (6% ± 2% and 5% ± 2%), lower prospective food consumption (5% ± 2% and 6% ± 2%), and lower desire to eat (7% ± 3% and 6% ± 3%) (all p < 0.05).
33203008	5	102	theme	%	957:957	arg1	%					962:962	7% ± 3% and 6% ± 3%	956:974	%	962:962	Compared to RG-I and control, FiberBind caused a higher composite satiety score (6% ± 2% and 5% ± 2%), lower prospective food consumption (5% ± 2% and 6% ± 2%), and lower desire to eat (7% ± 3% and 6% ± 3%) (all p < 0.05).
33086111	0	0	theme	polycaprolactone-chitosan	96:120	arg1	fibers					122:127	electrospun polycaprolactone-chitosan fibers	84:127	electrospun polycaprolactone-chitosan fibers	84:127	The response surface methodology for optimization of tyrosinase immobilization onto electrospun polycaprolactone-chitosan fibers for use in bisphenol A removal.
33086111	8	1	from	pH 7-8	1513:1518	arg1	%					1493:1493	80%	1491:1493	80% of bisphenol A at pH 7-8 and temperature range 15-35 °C	1491:1549	By contrast, free enzyme was able to remove over 80% of bisphenol A at pH 7-8 and temperature range 15-35 °C, and retained less than 60% of its initial activity after 30 days of storage.
33086111	8	1	from	pH 7-8	1513:1518	arg1	A					1508:1508	bisphenol A	1498:1508	bisphenol A at pH 7-8 and temperature range 15-35 °C	1498:1549	By contrast, free enzyme was able to remove over 80% of bisphenol A at pH 7-8 and temperature range 15-35 °C, and retained less than 60% of its initial activity after 30 days of storage.
33086111	6	2	from	concentration	1094:1106	arg1	solutions					1081:1089	solutions	1081:1089	solutions at concentration up to 3 mg/L	1081:1119	It was found that over 80% of the pollutant was removed after 120 min of the process, in the temperature range 15-45 °C and pH 6-9, using solutions at concentration up to 3 mg/L.
33086111	2	3	theme	enzyme	394:399	arg1	deposition					401:410	enzyme deposition	394:410	enzyme deposition	394:410	The morphology of the fibers and enzyme deposition were confirmed by SEM images.
33086111	0	4	theme	electrospun	84:94	arg1	fibers					122:127	electrospun polycaprolactone-chitosan fibers	84:127	electrospun polycaprolactone-chitosan fibers	84:127	The response surface methodology for optimization of tyrosinase immobilization onto electrospun polycaprolactone-chitosan fibers for use in bisphenol A removal.
33086111	7	5	theme	A	1411:1411	arg1	%					1396:1396	80%	1394:1396	80% of bisphenol A	1394:1411	Experimental data collected proved that the stability and reusability of the tyrosinase were significantly improved upon immobilization: the immobilized biomolecule retained around 90% of its initial activity after 30 days of storage, and was still capable to remove over 80% of bisphenol A even after 10 repeated uses.
33086111	7	5	theme	A	1411:1411	arg1	A					1411:1411	bisphenol A	1401:1411	bisphenol A	1401:1411	Experimental data collected proved that the stability and reusability of the tyrosinase were significantly improved upon immobilization: the immobilized biomolecule retained around 90% of its initial activity after 30 days of storage, and was still capable to remove over 80% of bisphenol A even after 10 repeated uses.
33086111	5	6	theme	remediation	919:929	arg1	conditions					931:940	various remediation conditions	911:940	various remediation conditions	911:940	Furthermore, as prepared system was applied in batch experiments related to biodegradation of bisphenol A under various remediation conditions.
33086111	1	7	theme	tyrosinase	292:301	arg1	immobilization					274:287	immobilization	274:287	immobilization of tyrosinase by mixed ionic interactions and hydrogen bonds formation	274:358	Composite polycaprolactone-chitosan material was produced by an electrospinning method and used as a support for immobilization of tyrosinase by mixed ionic interactions and hydrogen bonds formation.
33086111	7	8	theme	tyrosinase	1199:1208	arg1	stability					1166:1174	stability	1166:1174	stability	1166:1174	Experimental data collected proved that the stability and reusability of the tyrosinase were significantly improved upon immobilization: the immobilized biomolecule retained around 90% of its initial activity after 30 days of storage, and was still capable to remove over 80% of bisphenol A even after 10 repeated uses.
33086111	7	8	theme	tyrosinase	1199:1208	arg1	reusability					1180:1190	reusability	1180:1190	reusability	1180:1190	Experimental data collected proved that the stability and reusability of the tyrosinase were significantly improved upon immobilization: the immobilized biomolecule retained around 90% of its initial activity after 30 days of storage, and was still capable to remove over 80% of bisphenol A even after 10 repeated uses.
33086111	8	9	theme	activity	1594:1601	arg1	activity					1594:1601	its initial activity	1582:1601	its initial activity	1582:1601	By contrast, free enzyme was able to remove over 80% of bisphenol A at pH 7-8 and temperature range 15-35 °C, and retained less than 60% of its initial activity after 30 days of storage.
33086111	8	9	theme	activity	1594:1601	arg1	%					1577:1577	less than 60%	1565:1577	less than 60% of its initial activity	1565:1601	By contrast, free enzyme was able to remove over 80% of bisphenol A at pH 7-8 and temperature range 15-35 °C, and retained less than 60% of its initial activity after 30 days of storage.
33086111	5	10	theme	various	911:917	arg1	conditions					931:940	various remediation conditions	911:940	various remediation conditions	911:940	Furthermore, as prepared system was applied in batch experiments related to biodegradation of bisphenol A under various remediation conditions.
33086111	8	11	theme	storage	1620:1626	arg1	30 days					1609:1615	30 days	1609:1615	30 days of storage	1609:1626	By contrast, free enzyme was able to remove over 80% of bisphenol A at pH 7-8 and temperature range 15-35 °C, and retained less than 60% of its initial activity after 30 days of storage.
33086111	3	12	theme	optimal	543:549	arg1	conditions					551:560	optimal conditions	543:560	optimal conditions for immobilization process	543:587	Further, multivariate polynomial regression was used to model the experimental data and to determine optimal conditions for immobilization process, which were found to be pH 7, temperature 25 °C and 16 h process duration.
33086111	1	13	theme	mixed	306:310	arg1	interactions					318:329	mixed ionic interactions	306:329	mixed ionic interactions	306:329	Composite polycaprolactone-chitosan material was produced by an electrospinning method and used as a support for immobilization of tyrosinase by mixed ionic interactions and hydrogen bonds formation.
33086111	7	14	theme	immobilized	1263:1273	arg1	biomolecule					1275:1285	the immobilized biomolecule	1259:1285	the immobilized biomolecule	1259:1285	Experimental data collected proved that the stability and reusability of the tyrosinase were significantly improved upon immobilization: the immobilized biomolecule retained around 90% of its initial activity after 30 days of storage, and was still capable to remove over 80% of bisphenol A even after 10 repeated uses.
33086111	7	14	theme	immobilized	1263:1273	arg1	capable					1371:1377	capable	1371:1377	capable	1371:1377	Experimental data collected proved that the stability and reusability of the tyrosinase were significantly improved upon immobilization: the immobilized biomolecule retained around 90% of its initial activity after 30 days of storage, and was still capable to remove over 80% of bisphenol A even after 10 repeated uses.
33086111	7	15	theme	bisphenol	1401:1409	arg1	A					1411:1411	bisphenol A	1401:1411	bisphenol A	1401:1411	Experimental data collected proved that the stability and reusability of the tyrosinase were significantly improved upon immobilization: the immobilized biomolecule retained around 90% of its initial activity after 30 days of storage, and was still capable to remove over 80% of bisphenol A even after 10 repeated uses.
33086111	3	16	used	used	490:493	arg2	regression					475:484	multivariate polynomial regression	451:484	multivariate polynomial regression	451:484	Further, multivariate polynomial regression was used to model the experimental data and to determine optimal conditions for immobilization process, which were found to be pH 7, temperature 25 °C and 16 h process duration.
33086111	4	17	theme	biocatalytic	702:713	arg1	system					715:720	biocatalytic system	702:720	biocatalytic system	702:720	Under these conditions, novel type of biocatalytic system was produced with immobilization yield of 93% and expressed activity of 95%.
33086111	8	18	from	range	1536:1540	arg1	%					1493:1493	80%	1491:1493	80% of bisphenol A at pH 7-8 and temperature range 15-35 °C	1491:1549	By contrast, free enzyme was able to remove over 80% of bisphenol A at pH 7-8 and temperature range 15-35 °C, and retained less than 60% of its initial activity after 30 days of storage.
33086111	8	18	from	range	1536:1540	arg1	A					1508:1508	bisphenol A	1498:1508	bisphenol A at pH 7-8 and temperature range 15-35 °C	1498:1549	By contrast, free enzyme was able to remove over 80% of bisphenol A at pH 7-8 and temperature range 15-35 °C, and retained less than 60% of its initial activity after 30 days of storage.
33086111	7	19	theme	activity	1322:1329	arg1	activity					1322:1329	its initial activity	1310:1329	its initial activity	1310:1329	Experimental data collected proved that the stability and reusability of the tyrosinase were significantly improved upon immobilization: the immobilized biomolecule retained around 90% of its initial activity after 30 days of storage, and was still capable to remove over 80% of bisphenol A even after 10 repeated uses.
33086111	7	19	theme	activity	1322:1329	arg1	%					1305:1305	90%	1303:1305	90% of its initial activity	1303:1329	Experimental data collected proved that the stability and reusability of the tyrosinase were significantly improved upon immobilization: the immobilized biomolecule retained around 90% of its initial activity after 30 days of storage, and was still capable to remove over 80% of bisphenol A even after 10 repeated uses.
33086111	4	20	theme	%	796:796	arg1	yield					755:759	immobilization yield	740:759	immobilization yield of 93%	740:766	Under these conditions, novel type of biocatalytic system was produced with immobilization yield of 93% and expressed activity of 95%.
33086111	4	20	theme	%	796:796	arg1	activity					782:789	expressed activity	772:789	expressed activity of 95%	772:796	Under these conditions, novel type of biocatalytic system was produced with immobilization yield of 93% and expressed activity of 95%.
33086111	2	21	theme	SEM	430:432	arg1	images					434:439	SEM images	430:439	SEM images	430:439	The morphology of the fibers and enzyme deposition were confirmed by SEM images.
33086111	0	22	theme	response	4:11	arg1	methodology					21:31	The response surface methodology	0:31	The response surface methodology for optimization of tyrosinase immobilization onto electrospun polycaprolactone-chitosan fibers for use in bisphenol A removal.	0:159	The response surface methodology for optimization of tyrosinase immobilization onto electrospun polycaprolactone-chitosan fibers for use in bisphenol A removal.
33086111	3	23	theme	immobilization	566:579	arg1	process					581:587	immobilization process	566:587	immobilization process	566:587	Further, multivariate polynomial regression was used to model the experimental data and to determine optimal conditions for immobilization process, which were found to be pH 7, temperature 25 °C and 16 h process duration.
33086111	8	24	theme	A	1508:1508	arg1	%					1493:1493	80%	1491:1493	80% of bisphenol A at pH 7-8 and temperature range 15-35 °C	1491:1549	By contrast, free enzyme was able to remove over 80% of bisphenol A at pH 7-8 and temperature range 15-35 °C, and retained less than 60% of its initial activity after 30 days of storage.
33086111	8	24	theme	A	1508:1508	arg1	A					1508:1508	bisphenol A	1498:1508	bisphenol A at pH 7-8 and temperature range 15-35 °C	1498:1549	By contrast, free enzyme was able to remove over 80% of bisphenol A at pH 7-8 and temperature range 15-35 °C, and retained less than 60% of its initial activity after 30 days of storage.
33086111	3	25	theme	polynomial	464:473	arg1	regression					475:484	multivariate polynomial regression	451:484	multivariate polynomial regression	451:484	Further, multivariate polynomial regression was used to model the experimental data and to determine optimal conditions for immobilization process, which were found to be pH 7, temperature 25 °C and 16 h process duration.
33086111	1	26	theme	ionic	312:316	arg1	interactions					318:329	mixed ionic interactions	306:329	mixed ionic interactions	306:329	Composite polycaprolactone-chitosan material was produced by an electrospinning method and used as a support for immobilization of tyrosinase by mixed ionic interactions and hydrogen bonds formation.
33086111	4	27	theme	%	766:766	arg1	yield					755:759	immobilization yield	740:759	immobilization yield of 93%	740:766	Under these conditions, novel type of biocatalytic system was produced with immobilization yield of 93% and expressed activity of 95%.
33086111	4	27	theme	%	766:766	arg1	activity					782:789	expressed activity	772:789	expressed activity of 95%	772:796	Under these conditions, novel type of biocatalytic system was produced with immobilization yield of 93% and expressed activity of 95%.
33086111	1	28	theme	electrospinning	225:239	arg1	method					241:246	an electrospinning method	222:246	an electrospinning method	222:246	Composite polycaprolactone-chitosan material was produced by an electrospinning method and used as a support for immobilization of tyrosinase by mixed ionic interactions and hydrogen bonds formation.
33086111	6	29	theme	pollutant	977:985	arg1	%					968:968	over 80%	961:968	over 80% of the pollutant	961:985	It was found that over 80% of the pollutant was removed after 120 min of the process, in the temperature range 15-45 °C and pH 6-9, using solutions at concentration up to 3 mg/L.
33086111	6	29	theme	pollutant	977:985	arg1	pollutant					977:985	the pollutant	973:985	the pollutant	973:985	It was found that over 80% of the pollutant was removed after 120 min of the process, in the temperature range 15-45 °C and pH 6-9, using solutions at concentration up to 3 mg/L.
33086111	0	30	theme	A	150:150	arg1	removal					152:158	bisphenol A removal	140:158	bisphenol A removal	140:158	The response surface methodology for optimization of tyrosinase immobilization onto electrospun polycaprolactone-chitosan fibers for use in bisphenol A removal.
33086111	5	31	theme	batch	846:850	arg1	experiments					852:862	batch experiments	846:862	batch experiments related to biodegradation of bisphenol A under various remediation conditions	846:940	Furthermore, as prepared system was applied in batch experiments related to biodegradation of bisphenol A under various remediation conditions.
33086111	0	32	theme	surface	13:19	arg1	methodology					21:31	The response surface methodology	0:31	The response surface methodology for optimization of tyrosinase immobilization onto electrospun polycaprolactone-chitosan fibers for use in bisphenol A removal.	0:159	The response surface methodology for optimization of tyrosinase immobilization onto electrospun polycaprolactone-chitosan fibers for use in bisphenol A removal.
33086111	0	33	theme	bisphenol	140:148	arg1	removal					152:158	bisphenol A removal	140:158	bisphenol A removal	140:158	The response surface methodology for optimization of tyrosinase immobilization onto electrospun polycaprolactone-chitosan fibers for use in bisphenol A removal.
33086111	8	34	from	%	1493:1493	arg1	pH 7-8					1513:1518	pH 7-8	1513:1518	pH 7-8	1513:1518	By contrast, free enzyme was able to remove over 80% of bisphenol A at pH 7-8 and temperature range 15-35 °C, and retained less than 60% of its initial activity after 30 days of storage.
33086111	8	34	from	%	1493:1493	arg1	range					1536:1540	temperature range 15-35 °C	1524:1549	temperature range 15-35 °C	1524:1549	By contrast, free enzyme was able to remove over 80% of bisphenol A at pH 7-8 and temperature range 15-35 °C, and retained less than 60% of its initial activity after 30 days of storage.
33086111	4	35	theme	expressed	772:780	arg1	activity					782:789	expressed activity	772:789	expressed activity of 95%	772:796	Under these conditions, novel type of biocatalytic system was produced with immobilization yield of 93% and expressed activity of 95%.
33086111	3	36	theme	experimental	508:519	arg1	data					521:524	the experimental data	504:524	the experimental data	504:524	Further, multivariate polynomial regression was used to model the experimental data and to determine optimal conditions for immobilization process, which were found to be pH 7, temperature 25 °C and 16 h process duration.
33086111	1	37	theme	hydrogen	335:342	arg1	bonds					344:348	hydrogen bonds	335:348	hydrogen bonds formation	335:358	Composite polycaprolactone-chitosan material was produced by an electrospinning method and used as a support for immobilization of tyrosinase by mixed ionic interactions and hydrogen bonds formation.
33086111	6	38	theme	temperature	1036:1046	arg1	range					1048:1052	the temperature range 15-45 °C and pH 6-9	1032:1072	the temperature range 15-45 °C and pH 6-9	1032:1072	It was found that over 80% of the pollutant was removed after 120 min of the process, in the temperature range 15-45 °C and pH 6-9, using solutions at concentration up to 3 mg/L.
33086111	5	39	theme	A	903:903	arg1	biodegradation					875:888	biodegradation	875:888	biodegradation of bisphenol A under various remediation conditions	875:940	Furthermore, as prepared system was applied in batch experiments related to biodegradation of bisphenol A under various remediation conditions.
33086111	5	40	theme	related	864:870	arg1	experiments					852:862	batch experiments	846:862	batch experiments related to biodegradation of bisphenol A under various remediation conditions	846:940	Furthermore, as prepared system was applied in batch experiments related to biodegradation of bisphenol A under various remediation conditions.
33086111	3	41	theme	multivariate	451:462	arg1	regression					475:484	multivariate polynomial regression	451:484	multivariate polynomial regression	451:484	Further, multivariate polynomial regression was used to model the experimental data and to determine optimal conditions for immobilization process, which were found to be pH 7, temperature 25 °C and 16 h process duration.
33086111	1	42	theme	bonds	344:348	arg1	formation					350:358	hydrogen bonds formation	335:358	hydrogen bonds formation	335:358	Composite polycaprolactone-chitosan material was produced by an electrospinning method and used as a support for immobilization of tyrosinase by mixed ionic interactions and hydrogen bonds formation.
33086111	5	43	theme	bisphenol	893:901	arg1	A					903:903	bisphenol A	893:903	bisphenol A	893:903	Furthermore, as prepared system was applied in batch experiments related to biodegradation of bisphenol A under various remediation conditions.
33086111	1	44	used	used	252:255	arg2	material					197:204	Composite polycaprolactone-chitosan material	161:204	Composite polycaprolactone-chitosan material	161:204	Composite polycaprolactone-chitosan material was produced by an electrospinning method and used as a support for immobilization of tyrosinase by mixed ionic interactions and hydrogen bonds formation.
33086111	1	44	used	used	252:255	arg2	support					262:268	a support	260:268	a support for immobilization of tyrosinase by mixed ionic interactions and hydrogen bonds formation	260:358	Composite polycaprolactone-chitosan material was produced by an electrospinning method and used as a support for immobilization of tyrosinase by mixed ionic interactions and hydrogen bonds formation.
33086111	8	45	theme	initial	1586:1592	arg1	activity					1594:1601	its initial activity	1582:1601	its initial activity	1582:1601	By contrast, free enzyme was able to remove over 80% of bisphenol A at pH 7-8 and temperature range 15-35 °C, and retained less than 60% of its initial activity after 30 days of storage.
33086111	0	46	theme	tyrosinase	53:62	arg1	immobilization					64:77	tyrosinase immobilization	53:77	tyrosinase immobilization	53:77	The response surface methodology for optimization of tyrosinase immobilization onto electrospun polycaprolactone-chitosan fibers for use in bisphenol A removal.
33086111	7	47	dep	repeated	1427:1434	arg1	uses					1436:1439	uses	1436:1439	uses	1436:1439	Experimental data collected proved that the stability and reusability of the tyrosinase were significantly improved upon immobilization: the immobilized biomolecule retained around 90% of its initial activity after 30 days of storage, and was still capable to remove over 80% of bisphenol A even after 10 repeated uses.
33086111	8	48	theme	bisphenol	1498:1506	arg1	A					1508:1508	bisphenol A	1498:1508	bisphenol A at pH 7-8 and temperature range 15-35 °C	1498:1549	By contrast, free enzyme was able to remove over 80% of bisphenol A at pH 7-8 and temperature range 15-35 °C, and retained less than 60% of its initial activity after 30 days of storage.
33086111	2	49	theme	fibers	383:388	arg1	morphology					365:374	The morphology	361:374	The morphology of the fibers and enzyme deposition	361:410	The morphology of the fibers and enzyme deposition were confirmed by SEM images.
33086111	5	50	theme	prepared	815:822	arg1	system					824:829	as prepared system	812:829	as prepared system	812:829	Furthermore, as prepared system was applied in batch experiments related to biodegradation of bisphenol A under various remediation conditions.
33086111	8	51	theme	temperature	1524:1534	arg1	range					1536:1540	temperature range 15-35 °C	1524:1549	temperature range 15-35 °C	1524:1549	By contrast, free enzyme was able to remove over 80% of bisphenol A at pH 7-8 and temperature range 15-35 °C, and retained less than 60% of its initial activity after 30 days of storage.
33086111	3	52	theme	temperature	619:629	arg1	25 °C					631:635	temperature 25 °C	619:635	temperature 25 °C	619:635	Further, multivariate polynomial regression was used to model the experimental data and to determine optimal conditions for immobilization process, which were found to be pH 7, temperature 25 °C and 16 h process duration.
33086111	7	53	theme	storage	1348:1354	arg1	30 days					1337:1343	30 days	1337:1343	30 days of storage	1337:1354	Experimental data collected proved that the stability and reusability of the tyrosinase were significantly improved upon immobilization: the immobilized biomolecule retained around 90% of its initial activity after 30 days of storage, and was still capable to remove over 80% of bisphenol A even after 10 repeated uses.
33086111	4	54	theme	immobilization	740:753	arg1	yield					755:759	immobilization yield	740:759	immobilization yield of 93%	740:766	Under these conditions, novel type of biocatalytic system was produced with immobilization yield of 93% and expressed activity of 95%.
33086111	7	55	theme	Experimental	1122:1133	arg1	data					1135:1138	Experimental data	1122:1138	Experimental data collected	1122:1148	Experimental data collected proved that the stability and reusability of the tyrosinase were significantly improved upon immobilization: the immobilized biomolecule retained around 90% of its initial activity after 30 days of storage, and was still capable to remove over 80% of bisphenol A even after 10 repeated uses.
33086111	0	56	from	use	133:135	arg1	removal					152:158	bisphenol A removal	140:158	bisphenol A removal	140:158	The response surface methodology for optimization of tyrosinase immobilization onto electrospun polycaprolactone-chitosan fibers for use in bisphenol A removal.
33086111	4	57	theme	system	715:720	arg1	type					694:697	novel type	688:697	novel type of biocatalytic system	688:720	Under these conditions, novel type of biocatalytic system was produced with immobilization yield of 93% and expressed activity of 95%.
33086111	6	58	dep	range	1048:1052	arg1	pH 6-9					1067:1072	pH 6-9	1067:1072	pH 6-9	1067:1072	It was found that over 80% of the pollutant was removed after 120 min of the process, in the temperature range 15-45 °C and pH 6-9, using solutions at concentration up to 3 mg/L.
33086111	6	58	dep	range	1048:1052	arg1	15-45 °C					1054:1061	15-45 °C	1054:1061	15-45 °C	1054:1061	It was found that over 80% of the pollutant was removed after 120 min of the process, in the temperature range 15-45 °C and pH 6-9, using solutions at concentration up to 3 mg/L.
33086111	1	59	theme	polycaprolactone-chitosan	171:195	arg1	material					197:204	Composite polycaprolactone-chitosan material	161:204	Composite polycaprolactone-chitosan material	161:204	Composite polycaprolactone-chitosan material was produced by an electrospinning method and used as a support for immobilization of tyrosinase by mixed ionic interactions and hydrogen bonds formation.
33086111	1	59	theme	polycaprolactone-chitosan	171:195	arg1	support					262:268	a support	260:268	a support for immobilization of tyrosinase by mixed ionic interactions and hydrogen bonds formation	260:358	Composite polycaprolactone-chitosan material was produced by an electrospinning method and used as a support for immobilization of tyrosinase by mixed ionic interactions and hydrogen bonds formation.
33086111	7	60	dep	stability	1166:1174	arg1	the					1162:1164	the	1162:1164	the	1162:1164	Experimental data collected proved that the stability and reusability of the tyrosinase were significantly improved upon immobilization: the immobilized biomolecule retained around 90% of its initial activity after 30 days of storage, and was still capable to remove over 80% of bisphenol A even after 10 repeated uses.
33086111	8	61	theme	free	1455:1458	arg1	enzyme					1460:1465	free enzyme	1455:1465	free enzyme	1455:1465	By contrast, free enzyme was able to remove over 80% of bisphenol A at pH 7-8 and temperature range 15-35 °C, and retained less than 60% of its initial activity after 30 days of storage.
33086111	3	62	theme	16 h	641:644	arg1	duration					654:661	16 h process duration	641:661	16 h process duration	641:661	Further, multivariate polynomial regression was used to model the experimental data and to determine optimal conditions for immobilization process, which were found to be pH 7, temperature 25 °C and 16 h process duration.
33086111	7	63	theme	initial	1314:1320	arg1	activity					1322:1329	its initial activity	1310:1329	its initial activity	1310:1329	Experimental data collected proved that the stability and reusability of the tyrosinase were significantly improved upon immobilization: the immobilized biomolecule retained around 90% of its initial activity after 30 days of storage, and was still capable to remove over 80% of bisphenol A even after 10 repeated uses.
33086111	1	64	theme	Composite	161:169	arg1	material					197:204	Composite polycaprolactone-chitosan material	161:204	Composite polycaprolactone-chitosan material	161:204	Composite polycaprolactone-chitosan material was produced by an electrospinning method and used as a support for immobilization of tyrosinase by mixed ionic interactions and hydrogen bonds formation.
33086111	1	64	theme	Composite	161:169	arg1	support					262:268	a support	260:268	a support for immobilization of tyrosinase by mixed ionic interactions and hydrogen bonds formation	260:358	Composite polycaprolactone-chitosan material was produced by an electrospinning method and used as a support for immobilization of tyrosinase by mixed ionic interactions and hydrogen bonds formation.
33086111	4	65	theme	novel	688:692	arg1	type					694:697	novel type	688:697	novel type of biocatalytic system	688:720	Under these conditions, novel type of biocatalytic system was produced with immobilization yield of 93% and expressed activity of 95%.
33086111	6	66	theme	process	1020:1026	arg1	120 min					1005:1011	120 min	1005:1011	120 min of the process	1005:1026	It was found that over 80% of the pollutant was removed after 120 min of the process, in the temperature range 15-45 °C and pH 6-9, using solutions at concentration up to 3 mg/L.
33086111	0	67	theme	immobilization	64:77	arg1	optimization					37:48	optimization	37:48	optimization of tyrosinase immobilization onto electrospun polycaprolactone-chitosan fibers	37:127	The response surface methodology for optimization of tyrosinase immobilization onto electrospun polycaprolactone-chitosan fibers for use in bisphenol A removal.
33086111	3	68	theme	process	646:652	arg1	duration					654:661	16 h process duration	641:661	16 h process duration	641:661	Further, multivariate polynomial regression was used to model the experimental data and to determine optimal conditions for immobilization process, which were found to be pH 7, temperature 25 °C and 16 h process duration.
33086111	2	69	theme	deposition	401:410	arg1	morphology					365:374	The morphology	361:374	The morphology of the fibers and enzyme deposition	361:410	The morphology of the fibers and enzyme deposition were confirmed by SEM images.
31928547	3	0	from	composition	776:786	arg1	contents					802:809	intestinal contents	791:809	intestinal contents	791:809	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	0	from	composition	776:786	arg1	plasma					857:862	plasma	857:862	plasma	857:862	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	4	1	theme	proinflammatory	960:974	arg1	TNF-α					996:1000	TNF-α	996:1000	TNF-α	996:1000	Our results indicated that HP enhanced intestinal disaccharidase activities and reduced plasma proinflammatory cytokines including TNF-α and IL-6 in LPS-challenged piglets.
31928547	4	1	theme	proinflammatory	960:974	arg1	cytokines					976:984	plasma proinflammatory cytokines	953:984	plasma proinflammatory cytokines including TNF-α and IL-6 in LPS-challenged piglets	953:1035	Our results indicated that HP enhanced intestinal disaccharidase activities and reduced plasma proinflammatory cytokines including TNF-α and IL-6 in LPS-challenged piglets.
31928547	4	1	theme	proinflammatory	960:974	arg1	IL-6					1006:1009	IL-6	1006:1009	IL-6	1006:1009	Our results indicated that HP enhanced intestinal disaccharidase activities and reduced plasma proinflammatory cytokines including TNF-α and IL-6 in LPS-challenged piglets.
31928547	7	2	theme	SCFA	1504:1507	arg1	production					1509:1518	SCFA production	1504:1518	SCFA production	1504:1518	These results collectively suggest that HP is capable of alleviating LPS-triggered intestinal injury by improving intestinal disaccharidase activities, barrier function and SCFA production, while reducing intestinal inflammation.
31928547	3	3	with	feeding	424:430	arg1	diet					445:448	a basal diet	437:448	a basal diet supplemented with or without 250 mg/kg HP	437:490	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	1	4	theme	intestinal	164:173	arg1	inflammation					175:186	intestinal inflammation	164:186	intestinal inflammation	164:186	The effect of holly polyphenols (HP) on intestinal inflammation and microbiota composition was evaluated in a piglet model of lipopolysaccharide (LPS)-induced intestinal injury.
31928547	3	5	theme	levels	847:852	arg1	analysis					597:604	analysis	597:604	analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma	597:862	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	6	theme	composition	776:786	arg1	analysis					597:604	analysis	597:604	analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma	597:862	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	0	7	theme	lipopolysaccharide-challenged	88:116	arg1	pigs					118:121	lipopolysaccharide-challenged pigs	88:121	lipopolysaccharide-challenged pigs	88:121	Holly polyphenols alleviate intestinal inflammation and alter microbiota composition in lipopolysaccharide-challenged pigs.
31928547	6	8	theme	SCFA	1290:1293	arg1	production					1295:1304	SCFA production	1290:1304	SCFA production following LPS challenge	1290:1328	In addition, bacterial 16S rRNA gene sequencing showed that HP altered hindgut microbiota composition by enriching Prevotella and enhancing SCFA production following LPS challenge.
31928547	2	9	theme	×	350:350	arg1	design					364:369	a 2 × 2 factorial design	346:369	a 2 × 2 factorial design including diet type and LPS challenge	346:407	A total of twenty-four piglets were used in a 2 × 2 factorial design including diet type and LPS challenge.
31928547	4	10	theme	intestinal	904:913	arg1	activities					930:939	intestinal disaccharidase activities	904:939	intestinal disaccharidase activities	904:939	Our results indicated that HP enhanced intestinal disaccharidase activities and reduced plasma proinflammatory cytokines including TNF-α and IL-6 in LPS-challenged piglets.
31928547	3	11	theme	disaccharidase	609:622	arg1	activities					624:633	disaccharidase activities	609:633	disaccharidase activities	609:633	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	12	from	contents	802:809	arg1	analysis					597:604	analysis	597:604	analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma	597:862	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	6	13	theme	rRNA	1177:1180	arg1	sequencing					1187:1196	bacterial 16S rRNA gene sequencing	1163:1196	bacterial 16S rRNA gene sequencing	1163:1196	In addition, bacterial 16S rRNA gene sequencing showed that HP altered hindgut microbiota composition by enriching Prevotella and enhancing SCFA production following LPS challenge.
31928547	3	14	theme	proinflammatory	822:836	arg1	levels					847:852	proinflammatory cytokine levels	822:852	disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma	609:862	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	1	15	theme	-induced	274:281	arg1	injury					294:299	lipopolysaccharide (LPS)-induced intestinal injury	250:299	lipopolysaccharide (LPS)-induced intestinal injury	250:299	The effect of holly polyphenols (HP) on intestinal inflammation and microbiota composition was evaluated in a piglet model of lipopolysaccharide (LPS)-induced intestinal injury.
31928547	3	16	theme	levels	652:657	arg1	analysis					597:604	analysis	597:604	analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma	597:862	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	6	17	theme	16S	1173:1175	arg1	sequencing					1187:1196	bacterial 16S rRNA gene sequencing	1163:1196	bacterial 16S rRNA gene sequencing	1163:1196	In addition, bacterial 16S rRNA gene sequencing showed that HP altered hindgut microbiota composition by enriching Prevotella and enhancing SCFA production following LPS challenge.
31928547	3	18	theme	250	479:481	arg1	mg/kg					483:487	mg/kg	483:487	mg/kg	483:487	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	1	19	theme	intestinal	283:292	arg1	injury					294:299	lipopolysaccharide (LPS)-induced intestinal injury	250:299	lipopolysaccharide (LPS)-induced intestinal injury	250:299	The effect of holly polyphenols (HP) on intestinal inflammation and microbiota composition was evaluated in a piglet model of lipopolysaccharide (LPS)-induced intestinal injury.
31928547	5	20	theme	tight	1094:1098	arg1	occludin					1140:1147	occludin	1140:1147	occludin	1140:1147	Moreover, HP up-regulated mRNA expression of intestinal tight junction proteins such as claudin-1 and occludin.
31928547	5	20	theme	tight	1094:1098	arg1	proteins					1109:1116	intestinal tight junction proteins	1083:1116	intestinal tight junction proteins such as claudin-1 and occludin	1083:1147	Moreover, HP up-regulated mRNA expression of intestinal tight junction proteins such as claudin-1 and occludin.
31928547	5	20	theme	tight	1094:1098	arg1	claudin-1					1126:1134	claudin-1	1126:1134	claudin-1	1126:1134	Moreover, HP up-regulated mRNA expression of intestinal tight junction proteins such as claudin-1 and occludin.
31928547	7	21	theme	intestinal	1414:1423	arg1	injury					1425:1430	LPS-triggered intestinal injury	1400:1430	LPS-triggered intestinal injury	1400:1430	These results collectively suggest that HP is capable of alleviating LPS-triggered intestinal injury by improving intestinal disaccharidase activities, barrier function and SCFA production, while reducing intestinal inflammation.
31928547	7	22	theme	intestinal	1445:1454	arg1	activities					1471:1480	intestinal disaccharidase activities	1445:1480	intestinal disaccharidase activities	1445:1480	These results collectively suggest that HP is capable of alleviating LPS-triggered intestinal injury by improving intestinal disaccharidase activities, barrier function and SCFA production, while reducing intestinal inflammation.
31928547	6	23	theme	bacterial	1163:1171	arg1	sequencing					1187:1196	bacterial 16S rRNA gene sequencing	1163:1196	bacterial 16S rRNA gene sequencing	1163:1196	In addition, bacterial 16S rRNA gene sequencing showed that HP altered hindgut microbiota composition by enriching Prevotella and enhancing SCFA production following LPS challenge.
31928547	3	24	theme	microbiota	765:774	arg1	composition					776:786	microbiota composition	765:786	microbiota composition	765:786	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	25	theme	gene	636:639	arg1	levels					652:657	gene expression levels	636:657	gene expression levels of several representative tight junction proteins and inflammatory mediators	636:734	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	2	26	used	used	338:341	arg2	total					304:308	A total	302:308	A total of twenty-four piglets	302:331	A total of twenty-four piglets were used in a 2 × 2 factorial design including diet type and LPS challenge.
31928547	3	27	from	levels	847:852	arg1	contents					802:809	intestinal contents	791:809	intestinal contents	791:809	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	27	from	levels	847:852	arg1	plasma					857:862	plasma	857:862	plasma	857:862	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	1	28	theme	microbiota	192:201	arg1	composition					203:213	microbiota composition	192:213	microbiota composition	192:213	The effect of holly polyphenols (HP) on intestinal inflammation and microbiota composition was evaluated in a piglet model of lipopolysaccharide (LPS)-induced intestinal injury.
31928547	3	29	theme	expression	641:650	arg1	levels					652:657	gene expression levels	636:657	gene expression levels of several representative tight junction proteins and inflammatory mediators	636:734	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	30	theme	tight	685:689	arg1	proteins					700:707	several representative tight junction proteins	662:707	several representative tight junction proteins	662:707	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	2	31	theme	diet	381:384	arg1	type					386:389	diet type	381:389	diet type	381:389	A total of twenty-four piglets were used in a 2 × 2 factorial design including diet type and LPS challenge.
31928547	0	32	theme	Holly	0:4	arg1	polyphenols					6:16	Holly polyphenols	0:16	Holly polyphenols	0:16	Holly polyphenols alleviate intestinal inflammation and alter microbiota composition in lipopolysaccharide-challenged pigs.
31928547	1	33	theme	injury	294:299	arg1	model					241:245	a piglet model	232:245	a piglet model of lipopolysaccharide (LPS)-induced intestinal injury	232:299	The effect of holly polyphenols (HP) on intestinal inflammation and microbiota composition was evaluated in a piglet model of lipopolysaccharide (LPS)-induced intestinal injury.
31928547	7	34	theme	intestinal	1536:1545	arg1	inflammation					1547:1558	intestinal inflammation	1536:1558	intestinal inflammation	1536:1558	These results collectively suggest that HP is capable of alleviating LPS-triggered intestinal injury by improving intestinal disaccharidase activities, barrier function and SCFA production, while reducing intestinal inflammation.
31928547	2	35	theme	piglets	325:331	arg1	total					304:308	A total	302:308	A total of twenty-four piglets	302:331	A total of twenty-four piglets were used in a 2 × 2 factorial design including diet type and LPS challenge.
31928547	0	36	theme	intestinal	28:37	arg1	inflammation					39:50	intestinal inflammation	28:50	intestinal inflammation	28:50	Holly polyphenols alleviate intestinal inflammation and alter microbiota composition in lipopolysaccharide-challenged pigs.
31928547	3	37	theme	junction	691:698	arg1	proteins					700:707	several representative tight junction proteins	662:707	several representative tight junction proteins	662:707	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	4	38	theme	disaccharidase	915:928	arg1	activities					930:939	intestinal disaccharidase activities	904:939	intestinal disaccharidase activities	904:939	Our results indicated that HP enhanced intestinal disaccharidase activities and reduced plasma proinflammatory cytokines including TNF-α and IL-6 in LPS-challenged piglets.
31928547	7	39	theme	LPS-triggered	1400:1412	arg1	injury					1425:1430	LPS-triggered intestinal injury	1400:1430	LPS-triggered intestinal injury	1400:1430	These results collectively suggest that HP is capable of alleviating LPS-triggered intestinal injury by improving intestinal disaccharidase activities, barrier function and SCFA production, while reducing intestinal inflammation.
31928547	3	40	theme	basal	439:443	arg1	diet					445:448	a basal diet	437:448	a basal diet supplemented with or without 250 mg/kg HP	437:490	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	41	theme	μg/kg	528:532	arg1	weight					539:544	100 μg/kg body weight	524:544	100 μg/kg body weight	524:544	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	41	theme	μg/kg	528:532	arg1	LPS					519:521	LPS	519:521	LPS (100 μg/kg body weight)	519:545	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	42	theme	several	662:668	arg1	proteins					700:707	several representative tight junction proteins	662:707	several representative tight junction proteins	662:707	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	7	43	theme	disaccharidase	1456:1469	arg1	activities					1471:1480	intestinal disaccharidase activities	1445:1480	intestinal disaccharidase activities	1445:1480	These results collectively suggest that HP is capable of alleviating LPS-triggered intestinal injury by improving intestinal disaccharidase activities, barrier function and SCFA production, while reducing intestinal inflammation.
31928547	4	44	theme	plasma	953:958	arg1	TNF-α					996:1000	TNF-α	996:1000	TNF-α	996:1000	Our results indicated that HP enhanced intestinal disaccharidase activities and reduced plasma proinflammatory cytokines including TNF-α and IL-6 in LPS-challenged piglets.
31928547	4	44	theme	plasma	953:958	arg1	cytokines					976:984	plasma proinflammatory cytokines	953:984	plasma proinflammatory cytokines including TNF-α and IL-6 in LPS-challenged piglets	953:1035	Our results indicated that HP enhanced intestinal disaccharidase activities and reduced plasma proinflammatory cytokines including TNF-α and IL-6 in LPS-challenged piglets.
31928547	4	44	theme	plasma	953:958	arg1	IL-6					1006:1009	IL-6	1006:1009	IL-6	1006:1009	Our results indicated that HP enhanced intestinal disaccharidase activities and reduced plasma proinflammatory cytokines including TNF-α and IL-6 in LPS-challenged piglets.
31928547	3	45	theme	mg/kg	483:487	arg1	HP					489:490	250 mg/kg HP	479:490	250 mg/kg HP	479:490	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	4	46	from	IL-6	1006:1009	arg1	piglets					1029:1035	LPS-challenged piglets	1014:1035	LPS-challenged piglets	1014:1035	Our results indicated that HP enhanced intestinal disaccharidase activities and reduced plasma proinflammatory cytokines including TNF-α and IL-6 in LPS-challenged piglets.
31928547	3	47	theme	representative	670:683	arg1	proteins					700:707	several representative tight junction proteins	662:707	several representative tight junction proteins	662:707	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	48	from	analysis	597:604	arg1	contents					802:809	intestinal contents	791:809	intestinal contents	791:809	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	48	from	analysis	597:604	arg1	plasma					857:862	plasma	857:862	plasma	857:862	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	5	49	theme	mRNA	1064:1067	arg1	expression					1069:1078	mRNA expression	1064:1078	mRNA expression of intestinal tight junction proteins such as claudin-1 and occludin	1064:1147	Moreover, HP up-regulated mRNA expression of intestinal tight junction proteins such as claudin-1 and occludin.
31928547	6	50	theme	microbiota	1229:1238	arg1	composition					1240:1250	hindgut microbiota composition	1221:1250	hindgut microbiota composition	1221:1250	In addition, bacterial 16S rRNA gene sequencing showed that HP altered hindgut microbiota composition by enriching Prevotella and enhancing SCFA production following LPS challenge.
31928547	7	51	theme	barrier	1483:1489	arg1	function					1491:1498	barrier function	1483:1498	barrier function	1483:1498	These results collectively suggest that HP is capable of alleviating LPS-triggered intestinal injury by improving intestinal disaccharidase activities, barrier function and SCFA production, while reducing intestinal inflammation.
31928547	3	52	theme	inflammatory	713:724	arg1	mediators					726:734	inflammatory mediators	713:734	inflammatory mediators	713:734	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	53	theme	saline	569:574	arg1	volume					559:564	an equal volume	550:564	an equal volume of saline	550:574	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	53	theme	saline	569:574	arg1	weight					539:544	100 μg/kg body weight	524:544	100 μg/kg body weight	524:544	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	53	theme	saline	569:574	arg1	LPS					519:521	LPS	519:521	LPS (100 μg/kg body weight)	519:545	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	54	from	levels	652:657	arg1	contents					802:809	intestinal contents	791:809	intestinal contents	791:809	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	54	from	levels	652:657	arg1	plasma					857:862	plasma	857:862	plasma	857:862	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	1	55	theme	piglet	234:239	arg1	model					241:245	a piglet model	232:245	a piglet model of lipopolysaccharide (LPS)-induced intestinal injury	232:299	The effect of holly polyphenols (HP) on intestinal inflammation and microbiota composition was evaluated in a piglet model of lipopolysaccharide (LPS)-induced intestinal injury.
31928547	0	56	theme	microbiota	62:71	arg1	composition					73:83	microbiota composition	62:83	microbiota composition in lipopolysaccharide-challenged pigs	62:121	Holly polyphenols alleviate intestinal inflammation and alter microbiota composition in lipopolysaccharide-challenged pigs.
31928547	3	57	theme	mediators	726:734	arg1	activities					624:633	disaccharidase activities	609:633	disaccharidase activities	609:633	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	57	theme	mediators	726:734	arg1	levels					652:657	gene expression levels	636:657	gene expression levels of several representative tight junction proteins and inflammatory mediators	636:734	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	57	theme	mediators	726:734	arg1	concentrations					746:759	the SCFA concentrations	737:759	the SCFA concentrations	737:759	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	57	theme	mediators	726:734	arg1	composition					776:786	microbiota composition	765:786	microbiota composition	765:786	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	57	theme	mediators	726:734	arg1	levels					847:852	proinflammatory cytokine levels	822:852	disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma	609:862	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	5	58	theme	intestinal	1083:1092	arg1	occludin					1140:1147	occludin	1140:1147	occludin	1140:1147	Moreover, HP up-regulated mRNA expression of intestinal tight junction proteins such as claudin-1 and occludin.
31928547	5	58	theme	intestinal	1083:1092	arg1	proteins					1109:1116	intestinal tight junction proteins	1083:1116	intestinal tight junction proteins such as claudin-1 and occludin	1083:1147	Moreover, HP up-regulated mRNA expression of intestinal tight junction proteins such as claudin-1 and occludin.
31928547	5	58	theme	intestinal	1083:1092	arg1	claudin-1					1126:1134	claudin-1	1126:1134	claudin-1	1126:1134	Moreover, HP up-regulated mRNA expression of intestinal tight junction proteins such as claudin-1 and occludin.
31928547	0	59	from	composition	73:83	arg1	pigs					118:121	lipopolysaccharide-challenged pigs	88:121	lipopolysaccharide-challenged pigs	88:121	Holly polyphenols alleviate intestinal inflammation and alter microbiota composition in lipopolysaccharide-challenged pigs.
31928547	3	60	theme	body	534:537	arg1	weight					539:544	100 μg/kg body weight	524:544	100 μg/kg body weight	524:544	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	60	theme	body	534:537	arg1	LPS					519:521	LPS	519:521	LPS (100 μg/kg body weight)	519:545	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	5	61	theme	junction	1100:1107	arg1	occludin					1140:1147	occludin	1140:1147	occludin	1140:1147	Moreover, HP up-regulated mRNA expression of intestinal tight junction proteins such as claudin-1 and occludin.
31928547	5	61	theme	junction	1100:1107	arg1	proteins					1109:1116	intestinal tight junction proteins	1083:1116	intestinal tight junction proteins such as claudin-1 and occludin	1083:1147	Moreover, HP up-regulated mRNA expression of intestinal tight junction proteins such as claudin-1 and occludin.
31928547	5	61	theme	junction	1100:1107	arg1	claudin-1					1126:1134	claudin-1	1126:1134	claudin-1	1126:1134	Moreover, HP up-regulated mRNA expression of intestinal tight junction proteins such as claudin-1 and occludin.
31928547	1	62	theme	holly	138:142	arg1	HP					157:158	HP	157:158	HP	157:158	The effect of holly polyphenols (HP) on intestinal inflammation and microbiota composition was evaluated in a piglet model of lipopolysaccharide (LPS)-induced intestinal injury.
31928547	1	62	theme	holly	138:142	arg1	polyphenols					144:154	holly polyphenols	138:154	holly polyphenols (HP)	138:159	The effect of holly polyphenols (HP) on intestinal inflammation and microbiota composition was evaluated in a piglet model of lipopolysaccharide (LPS)-induced intestinal injury.
31928547	3	63	theme	proteins	700:707	arg1	activities					624:633	disaccharidase activities	609:633	disaccharidase activities	609:633	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	63	theme	proteins	700:707	arg1	levels					652:657	gene expression levels	636:657	gene expression levels of several representative tight junction proteins and inflammatory mediators	636:734	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	63	theme	proteins	700:707	arg1	concentrations					746:759	the SCFA concentrations	737:759	the SCFA concentrations	737:759	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	63	theme	proteins	700:707	arg1	composition					776:786	microbiota composition	765:786	microbiota composition	765:786	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	63	theme	proteins	700:707	arg1	levels					847:852	proinflammatory cytokine levels	822:852	disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma	609:862	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	5	64	theme	proteins	1109:1116	arg1	expression					1069:1078	mRNA expression	1064:1078	mRNA expression of intestinal tight junction proteins such as claudin-1 and occludin	1064:1147	Moreover, HP up-regulated mRNA expression of intestinal tight junction proteins such as claudin-1 and occludin.
31928547	1	65	theme	polyphenols	144:154	arg1	effect					128:133	The effect	124:133	The effect of holly polyphenols (HP) on intestinal inflammation and microbiota composition	124:213	The effect of holly polyphenols (HP) on intestinal inflammation and microbiota composition was evaluated in a piglet model of lipopolysaccharide (LPS)-induced intestinal injury.
31928547	1	66	from	effect	128:133	arg1	composition					203:213	microbiota composition	192:213	microbiota composition	192:213	The effect of holly polyphenols (HP) on intestinal inflammation and microbiota composition was evaluated in a piglet model of lipopolysaccharide (LPS)-induced intestinal injury.
31928547	1	66	from	effect	128:133	arg1	inflammation					175:186	intestinal inflammation	164:186	intestinal inflammation	164:186	The effect of holly polyphenols (HP) on intestinal inflammation and microbiota composition was evaluated in a piglet model of lipopolysaccharide (LPS)-induced intestinal injury.
31928547	6	67	theme	gene	1182:1185	arg1	sequencing					1187:1196	bacterial 16S rRNA gene sequencing	1163:1196	bacterial 16S rRNA gene sequencing	1163:1196	In addition, bacterial 16S rRNA gene sequencing showed that HP altered hindgut microbiota composition by enriching Prevotella and enhancing SCFA production following LPS challenge.
31928547	6	68	theme	LPS	1316:1318	arg1	challenge					1320:1328	LPS challenge	1316:1328	LPS challenge	1316:1328	In addition, bacterial 16S rRNA gene sequencing showed that HP altered hindgut microbiota composition by enriching Prevotella and enhancing SCFA production following LPS challenge.
31928547	4	69	theme	LPS-challenged	1014:1027	arg1	piglets					1029:1035	LPS-challenged piglets	1014:1035	LPS-challenged piglets	1014:1035	Our results indicated that HP enhanced intestinal disaccharidase activities and reduced plasma proinflammatory cytokines including TNF-α and IL-6 in LPS-challenged piglets.
31928547	6	70	theme	hindgut	1221:1227	arg1	composition					1240:1250	hindgut microbiota composition	1221:1250	hindgut microbiota composition	1221:1250	In addition, bacterial 16S rRNA gene sequencing showed that HP altered hindgut microbiota composition by enriching Prevotella and enhancing SCFA production following LPS challenge.
31928547	3	71	theme	SCFA	741:744	arg1	concentrations					746:759	the SCFA concentrations	737:759	the SCFA concentrations	737:759	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	72	from	concentrations	746:759	arg1	contents					802:809	intestinal contents	791:809	intestinal contents	791:809	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	72	from	concentrations	746:759	arg1	plasma					857:862	plasma	857:862	plasma	857:862	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	73	from	plasma	857:862	arg1	analysis					597:604	analysis	597:604	analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma	597:862	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	74	theme	intestinal	791:800	arg1	contents					802:809	intestinal contents	791:809	intestinal contents	791:809	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	75	theme	concentrations	746:759	arg1	analysis					597:604	analysis	597:604	analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma	597:862	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	2	76	theme	LPS	395:397	arg1	challenge					399:407	LPS challenge	395:407	LPS challenge	395:407	A total of twenty-four piglets were used in a 2 × 2 factorial design including diet type and LPS challenge.
31928547	3	77	theme	feeding	424:430	arg1	d					419:419	16 d	416:419	16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP	416:490	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	78	from	activities	624:633	arg1	contents					802:809	intestinal contents	791:809	intestinal contents	791:809	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	78	from	activities	624:633	arg1	plasma					857:862	plasma	857:862	plasma	857:862	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	79	theme	activities	624:633	arg1	analysis					597:604	analysis	597:604	analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma	597:862	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	3	80	theme	equal	553:557	arg1	volume					559:564	an equal volume	550:564	an equal volume of saline	550:574	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	2	81	theme	factorial	354:362	arg1	design					364:369	a 2 × 2 factorial design	346:369	a 2 × 2 factorial design including diet type and LPS challenge	346:407	A total of twenty-four piglets were used in a 2 × 2 factorial design including diet type and LPS challenge.
31928547	3	82	theme	cytokine	838:845	arg1	levels					847:852	proinflammatory cytokine levels	822:852	disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma	609:862	After 16 d of feeding with a basal diet supplemented with or without 250 mg/kg HP, pigs were challenged with LPS (100 μg/kg body weight) or an equal volume of saline for 4 h, followed by analysis of disaccharidase activities, gene expression levels of several representative tight junction proteins and inflammatory mediators, the SCFA concentrations and microbiota composition in intestinal contents as well as proinflammatory cytokine levels in plasma.
31928547	4	83	from	TNF-α	996:1000	arg1	piglets					1029:1035	LPS-challenged piglets	1014:1035	LPS-challenged piglets	1014:1035	Our results indicated that HP enhanced intestinal disaccharidase activities and reduced plasma proinflammatory cytokines including TNF-α and IL-6 in LPS-challenged piglets.
32840368	2	0	theme	located	688:694	arg1	chains					681:686	fatty acyl chains	670:686	fatty acyl chains located in sn-1/sn-2 position on the glycerol backbone (O-linked)	670:752	From an analytical point of view, the critical aspect of these bioactive lipids lies in the determination of fatty acyl chains located in sn-1/sn-2 position on the glycerol backbone (O-linked), along with the amide-bound (N-linked) fatty acyl chain.
32840368	3	1	dep	high-	1064:1068	arg1	trap					1112:1115	linear ion trap	1101:1115	linear ion trap	1101:1115	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	3	1	dep	high-	1064:1068	arg1	FTMS					1086:1089	Orbitrap FTMS	1077:1089	Orbitrap FTMS	1077:1089	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	3	1	dep	high-	1064:1068	arg1	LIT					1118:1120	LIT	1118:1120	LIT	1118:1120	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	1	2	theme	membranes	334:342	arg1	constituents					304:315	important constituents	294:315	important constituents of intracellular membranes of plant tissues	294:359	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines (NAPE) are low abundance phospholipids but important constituents of intracellular membranes of plant tissues, responsible for generating bioactive N-acylethanolamine (NAE), which participates in several physiological processes such as regulation of seed germination and protection against pathogenic attacks.
32840368	2	3	theme	acyl	676:679	arg1	chains					681:686	fatty acyl chains	670:686	fatty acyl chains located in sn-1/sn-2 position on the glycerol backbone (O-linked)	670:752	From an analytical point of view, the critical aspect of these bioactive lipids lies in the determination of fatty acyl chains located in sn-1/sn-2 position on the glycerol backbone (O-linked), along with the amide-bound (N-linked) fatty acyl chain.
32840368	4	4	theme	CID	1178:1180	arg1	MS					1190:1191	Collisional induced dissociation (CID)-tandem MS and MS3 acquisitions	1144:1212	MS	1190:1191	Collisional induced dissociation (CID)-tandem MS and MS3 acquisitions of chemically prepared NAPE allowed to unequivocally recognize the N-linked fatty acyl chain and to establish the diagnostic product ions that were successfully applied to identify NAPE in lipid extracts of yellow lupin seeds.
32840368	1	5	theme	pathogenic	541:550	arg1	attacks					552:558	pathogenic attacks	541:558	pathogenic attacks	541:558	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines (NAPE) are low abundance phospholipids but important constituents of intracellular membranes of plant tissues, responsible for generating bioactive N-acylethanolamine (NAE), which participates in several physiological processes such as regulation of seed germination and protection against pathogenic attacks.
32840368	1	6	theme	plant	347:351	arg1	tissues					353:359	plant tissues	347:359	plant tissues	347:359	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines (NAPE) are low abundance phospholipids but important constituents of intracellular membranes of plant tissues, responsible for generating bioactive N-acylethanolamine (NAE), which participates in several physiological processes such as regulation of seed germination and protection against pathogenic attacks.
32840368	2	7	theme	fatty	793:797	arg1	chain					804:808	the amide-bound (N-linked) fatty acyl chain	766:808	the amide-bound (N-linked) fatty acyl chain	766:808	From an analytical point of view, the critical aspect of these bioactive lipids lies in the determination of fatty acyl chains located in sn-1/sn-2 position on the glycerol backbone (O-linked), along with the amide-bound (N-linked) fatty acyl chain.
32840368	7	8	theme	total	2003:2007	arg1	NAPE					2019:2022	NAPE	2019:2022	NAPE	2019:2022	The total amount of NAPE in lupin seeds ranged in the interval of 2.00 ± 0.13 mg/g dw, in agreement with other edible legumes.
32840368	7	8	theme	total	2003:2007	arg1	amount					2009:2014	The total amount	1999:2014	The total amount of NAPE in lupin seeds	1999:2037	The total amount of NAPE in lupin seeds ranged in the interval of 2.00 ± 0.13 mg/g dw, in agreement with other edible legumes.
32840368	8	9	theme	robust	2161:2166	arg1	method					2179:2184	a robust assessment method	2159:2184	a robust assessment method potentially applicable to biological extracts containing NAPE species	2159:2254	We anticipate our approach to be a robust assessment method potentially applicable to biological extracts containing NAPE species and can provide comprehensive profiles and contents.
32840368	2	10	theme	sn-1/sn-2	699:707	arg1	position					709:716	sn-1/sn-2 position	699:716	sn-1/sn-2 position	699:716	From an analytical point of view, the critical aspect of these bioactive lipids lies in the determination of fatty acyl chains located in sn-1/sn-2 position on the glycerol backbone (O-linked), along with the amide-bound (N-linked) fatty acyl chain.
32840368	5	11	theme	acyl	1600:1603	arg1	chains					1605:1610	almost the same acyl chains	1584:1610	almost the same acyl chains O-linked on the glycerol backbone in several sn-1/sn-2 combinations	1584:1678	The most abundant NAPE species were those containing N-acyl groups C18:1, C18:2; a minor prevalence was found for C16:0, C18:0, and C18:3, and almost the same acyl chains O-linked on the glycerol backbone in several sn-1/sn-2 combinations were observed.
32840368	6	12	from	m/z	1788:1790	arg1	54:3					1952:1955	two isomers 54:3	1940:1955	two isomers 54:3	1940:1955	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	12	from	m/z	1788:1790	arg1	m/z					1891:1893	m/z 1004.7686	1891:1903	m/z 1004.7686 (two isomers 54:4)	1891:1922	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	12	from	m/z	1788:1790	arg1	molecules					1766:1774	deprotonated molecules	1753:1774	deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2)	1753:1996	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	12	from	m/z	1788:1790	arg1	52:2					1849:1852	two isomers 52:2	1837:1852	two isomers 52:2	1837:1852	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	12	from	m/z	1788:1790	arg1	m/z					1925:1927	m/z 1006.7837	1925:1937	m/z 1006.7837 (two isomers 54:3)	1925:1956	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	12	from	m/z	1788:1790	arg1	54:2					1992:1995	single isomer 54:2	1978:1995	single isomer 54:2	1978:1995	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	12	from	m/z	1788:1790	arg1	54:5					1884:1887	four isomers 54:5	1871:1887	four isomers 54:5	1871:1887	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	12	from	m/z	1788:1790	arg1	m/z					1823:1825	m/z 980.7694	1823:1834	m/z 980.7694 (two isomers 52:2)	1823:1853	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	12	from	m/z	1788:1790	arg1	m/z					1963:1965	m/z 1008.8026	1963:1975	m/z 1008.8026 (single isomer 54:2)	1963:1996	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	12	from	m/z	1788:1790	arg1	54:4					1918:1921	two isomers 54:4	1906:1921	two isomers 54:4	1906:1921	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	12	from	m/z	1788:1790	arg1	m/z					1856:1858	m/z 1002.7535	1856:1868	m/z 1002.7535 (four isomers 54:5)	1856:1888	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	5	13	theme	O-linked	1612:1619	arg1	chains					1605:1610	almost the same acyl chains	1584:1610	almost the same acyl chains O-linked on the glycerol backbone in several sn-1/sn-2 combinations	1584:1678	The most abundant NAPE species were those containing N-acyl groups C18:1, C18:2; a minor prevalence was found for C16:0, C18:0, and C18:3, and almost the same acyl chains O-linked on the glycerol backbone in several sn-1/sn-2 combinations were observed.
32840368	4	14	theme	lipid	1403:1407	arg1	extracts					1409:1416	lipid extracts	1403:1416	lipid extracts of yellow lupin seeds	1403:1438	Collisional induced dissociation (CID)-tandem MS and MS3 acquisitions of chemically prepared NAPE allowed to unequivocally recognize the N-linked fatty acyl chain and to establish the diagnostic product ions that were successfully applied to identify NAPE in lipid extracts of yellow lupin seeds.
32840368	1	15	theme	low	262:264	arg1	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines					198:249	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines	198:249	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines (NAPE)	198:256	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines (NAPE) are low abundance phospholipids but important constituents of intracellular membranes of plant tissues, responsible for generating bioactive N-acylethanolamine (NAE), which participates in several physiological processes such as regulation of seed germination and protection against pathogenic attacks.
32840368	1	15	theme	low	262:264	arg1	phospholipids					276:288	low abundance phospholipids	262:288	low abundance phospholipids	262:288	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines (NAPE) are low abundance phospholipids but important constituents of intracellular membranes of plant tissues, responsible for generating bioactive N-acylethanolamine (NAE), which participates in several physiological processes such as regulation of seed germination and protection against pathogenic attacks.
32840368	8	16	theme	applicable	2198:2207	arg1	method					2179:2184	a robust assessment method	2159:2184	a robust assessment method potentially applicable to biological extracts containing NAPE species	2159:2254	We anticipate our approach to be a robust assessment method potentially applicable to biological extracts containing NAPE species and can provide comprehensive profiles and contents.
32840368	2	17	theme	bioactive	624:632	arg1	lipids					634:639	these bioactive lipids	618:639	these bioactive lipids	618:639	From an analytical point of view, the critical aspect of these bioactive lipids lies in the determination of fatty acyl chains located in sn-1/sn-2 position on the glycerol backbone (O-linked), along with the amide-bound (N-linked) fatty acyl chain.
32840368	6	18	theme	52:3	1816:1819	arg1	isomers					1808:1814	three isomers 52:3	1802:1819	three isomers 52:3	1802:1819	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	18	theme	52:3	1816:1819	arg1	m/z					1788:1790	m/z 978.7541	1788:1799	m/z 978.7541 (three isomers 52:3)	1788:1820	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	2	19	dep	located	688:694	arg1	O-linked					744:751	O-linked	744:751	O-linked	744:751	From an analytical point of view, the critical aspect of these bioactive lipids lies in the determination of fatty acyl chains located in sn-1/sn-2 position on the glycerol backbone (O-linked), along with the amide-bound (N-linked) fatty acyl chain.
32840368	8	20	theme	biological	2212:2221	arg1	extracts					2223:2230	biological extracts	2212:2230	biological extracts containing NAPE species	2212:2254	We anticipate our approach to be a robust assessment method potentially applicable to biological extracts containing NAPE species and can provide comprehensive profiles and contents.
32840368	2	21	theme	amide-bound	770:780	arg1	chain					804:808	the amide-bound (N-linked) fatty acyl chain	766:808	the amide-bound (N-linked) fatty acyl chain	766:808	From an analytical point of view, the critical aspect of these bioactive lipids lies in the determination of fatty acyl chains located in sn-1/sn-2 position on the glycerol backbone (O-linked), along with the amide-bound (N-linked) fatty acyl chain.
32840368	4	22	theme	-tandem	1182:1188	arg1	MS					1190:1191	Collisional induced dissociation (CID)-tandem MS and MS3 acquisitions	1144:1212	MS	1190:1191	Collisional induced dissociation (CID)-tandem MS and MS3 acquisitions of chemically prepared NAPE allowed to unequivocally recognize the N-linked fatty acyl chain and to establish the diagnostic product ions that were successfully applied to identify NAPE in lipid extracts of yellow lupin seeds.
32840368	0	23	theme	Seeds	191:195	arg1	Extracts					173:180	Extracts	173:180	Extracts of Lupin Seeds	173:195	Regiochemical Assignment of N-Acylphosphatidylethanolamines (NAPE) by Liquid Chromatography/Electrospray Ionization with Multistage Mass Spectrometry and Its Application to Extracts of Lupin Seeds.
32840368	1	24	theme	germination	506:516	arg1	protection					522:531	protection	522:531	protection against pathogenic attacks	522:558	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines (NAPE) are low abundance phospholipids but important constituents of intracellular membranes of plant tissues, responsible for generating bioactive N-acylethanolamine (NAE), which participates in several physiological processes such as regulation of seed germination and protection against pathogenic attacks.
32840368	1	24	theme	germination	506:516	arg1	regulation					487:496	regulation	487:496	regulation of seed germination	487:516	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines (NAPE) are low abundance phospholipids but important constituents of intracellular membranes of plant tissues, responsible for generating bioactive N-acylethanolamine (NAE), which participates in several physiological processes such as regulation of seed germination and protection against pathogenic attacks.
32840368	4	25	theme	Collisional	1144:1154	arg1	MS					1190:1191	Collisional induced dissociation (CID)-tandem MS and MS3 acquisitions	1144:1212	MS	1190:1191	Collisional induced dissociation (CID)-tandem MS and MS3 acquisitions of chemically prepared NAPE allowed to unequivocally recognize the N-linked fatty acyl chain and to establish the diagnostic product ions that were successfully applied to identify NAPE in lipid extracts of yellow lupin seeds.
32840368	5	26	contain	containing	1483:1492	arg2	groups					1501:1506	N-acyl groups C18:1, C18:2	1494:1519	N-acyl groups C18:1, C18:2	1494:1519	The most abundant NAPE species were those containing N-acyl groups C18:1, C18:2; a minor prevalence was found for C16:0, C18:0, and C18:3, and almost the same acyl chains O-linked on the glycerol backbone in several sn-1/sn-2 combinations were observed.
32840368	5	26	contain	containing	1483:1492	arg1	species					1464:1470	The most abundant NAPE species	1441:1470	The most abundant NAPE species	1441:1470	The most abundant NAPE species were those containing N-acyl groups C18:1, C18:2; a minor prevalence was found for C16:0, C18:0, and C18:3, and almost the same acyl chains O-linked on the glycerol backbone in several sn-1/sn-2 combinations were observed.
32840368	5	26	contain	containing	1483:1492	arg1	those					1477:1481	those	1477:1481	those	1477:1481	The most abundant NAPE species were those containing N-acyl groups C18:1, C18:2; a minor prevalence was found for C16:0, C18:0, and C18:3, and almost the same acyl chains O-linked on the glycerol backbone in several sn-1/sn-2 combinations were observed.
32840368	0	27	theme	Liquid	70:75	arg1	Spectrometry					137:148	Liquid Chromatography/Electrospray Ionization with Multistage Mass Spectrometry	70:148	Liquid Chromatography/Electrospray Ionization with Multistage Mass Spectrometry	70:148	Regiochemical Assignment of N-Acylphosphatidylethanolamines (NAPE) by Liquid Chromatography/Electrospray Ionization with Multistage Mass Spectrometry and Its Application to Extracts of Lupin Seeds.
32840368	2	28	theme	N-linked	783:790	arg1	chain					804:808	the amide-bound (N-linked) fatty acyl chain	766:808	the amide-bound (N-linked) fatty acyl chain	766:808	From an analytical point of view, the critical aspect of these bioactive lipids lies in the determination of fatty acyl chains located in sn-1/sn-2 position on the glycerol backbone (O-linked), along with the amide-bound (N-linked) fatty acyl chain.
32840368	3	29	theme	lupin	874:878	arg1	Lupinus					887:893	Lupinus	887:893	Lupinus	887:893	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	3	29	theme	lupin	874:878	arg1	seeds					880:884	lupin seeds	874:884	lupin seeds (Lupinus luteus L.)	874:904	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	0	30	theme	Ionization	105:114	arg1	Spectrometry					137:148	Liquid Chromatography/Electrospray Ionization with Multistage Mass Spectrometry	70:148	Liquid Chromatography/Electrospray Ionization with Multistage Mass Spectrometry	70:148	Regiochemical Assignment of N-Acylphosphatidylethanolamines (NAPE) by Liquid Chromatography/Electrospray Ionization with Multistage Mass Spectrometry and Its Application to Extracts of Lupin Seeds.
32840368	8	31	theme	comprehensive	2272:2284	arg1	profiles					2286:2293	comprehensive profiles	2272:2293	comprehensive profiles	2272:2293	We anticipate our approach to be a robust assessment method potentially applicable to biological extracts containing NAPE species and can provide comprehensive profiles and contents.
32840368	6	32	theme	positional	1699:1708	arg1	isomers					1710:1716	The positional isomers	1695:1716	The positional isomers of NAPE species	1695:1732	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	32	theme	positional	1699:1708	arg1	molecules					1766:1774	deprotonated molecules	1753:1774	deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2)	1753:1996	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	5	33	theme	N-acyl	1494:1499	arg1	groups					1501:1506	N-acyl groups C18:1, C18:2	1494:1519	N-acyl groups C18:1, C18:2	1494:1519	The most abundant NAPE species were those containing N-acyl groups C18:1, C18:2; a minor prevalence was found for C16:0, C18:0, and C18:3, and almost the same acyl chains O-linked on the glycerol backbone in several sn-1/sn-2 combinations were observed.
32840368	7	34	theme	dw	2082:2083	arg1	interval					2053:2060	the interval	2049:2060	the interval of 2.00 ± 0.13 mg/g dw	2049:2083	The total amount of NAPE in lupin seeds ranged in the interval of 2.00 ± 0.13 mg/g dw, in agreement with other edible legumes.
32840368	4	35	theme	lupin	1428:1432	arg1	seeds					1434:1438	yellow lupin seeds	1421:1438	yellow lupin seeds	1421:1438	Collisional induced dissociation (CID)-tandem MS and MS3 acquisitions of chemically prepared NAPE allowed to unequivocally recognize the N-linked fatty acyl chain and to establish the diagnostic product ions that were successfully applied to identify NAPE in lipid extracts of yellow lupin seeds.
32840368	0	36	theme	Multistage	121:130	arg1	Spectrometry					137:148	Liquid Chromatography/Electrospray Ionization with Multistage Mass Spectrometry	70:148	Liquid Chromatography/Electrospray Ionization with Multistage Mass Spectrometry	70:148	Regiochemical Assignment of N-Acylphosphatidylethanolamines (NAPE) by Liquid Chromatography/Electrospray Ionization with Multistage Mass Spectrometry and Its Application to Extracts of Lupin Seeds.
32840368	5	37	attach	O-linked	1612:1619	arg3	backbone					1637:1644	the glycerol backbone	1624:1644	the glycerol backbone in several sn-1/sn-2 combinations	1624:1678	The most abundant NAPE species were those containing N-acyl groups C18:1, C18:2; a minor prevalence was found for C16:0, C18:0, and C18:3, and almost the same acyl chains O-linked on the glycerol backbone in several sn-1/sn-2 combinations were observed.
32840368	2	38	theme	critical	599:606	arg1	aspect					608:613	the critical aspect	595:613	the critical aspect of these bioactive lipids	595:639	From an analytical point of view, the critical aspect of these bioactive lipids lies in the determination of fatty acyl chains located in sn-1/sn-2 position on the glycerol backbone (O-linked), along with the amide-bound (N-linked) fatty acyl chain.
32840368	7	39	theme	other	2104:2108	arg1	legumes					2117:2123	other edible legumes	2104:2123	other edible legumes	2104:2123	The total amount of NAPE in lupin seeds ranged in the interval of 2.00 ± 0.13 mg/g dw, in agreement with other edible legumes.
32840368	5	40	theme	abundant	1450:1457	arg1	species					1464:1470	The most abundant NAPE species	1441:1470	The most abundant NAPE species	1441:1470	The most abundant NAPE species were those containing N-acyl groups C18:1, C18:2; a minor prevalence was found for C16:0, C18:0, and C18:3, and almost the same acyl chains O-linked on the glycerol backbone in several sn-1/sn-2 combinations were observed.
32840368	5	40	theme	abundant	1450:1457	arg1	those					1477:1481	those	1477:1481	those	1477:1481	The most abundant NAPE species were those containing N-acyl groups C18:1, C18:2; a minor prevalence was found for C16:0, C18:0, and C18:3, and almost the same acyl chains O-linked on the glycerol backbone in several sn-1/sn-2 combinations were observed.
32840368	4	41	theme	NAPE	1237:1240	arg1	acquisitions					1201:1212	Collisional induced dissociation (CID)-tandem MS and MS3 acquisitions	1144:1212	acquisitions	1201:1212	Collisional induced dissociation (CID)-tandem MS and MS3 acquisitions of chemically prepared NAPE allowed to unequivocally recognize the N-linked fatty acyl chain and to establish the diagnostic product ions that were successfully applied to identify NAPE in lipid extracts of yellow lupin seeds.
32840368	4	41	theme	NAPE	1237:1240	arg1	MS					1190:1191	Collisional induced dissociation (CID)-tandem MS and MS3 acquisitions	1144:1212	MS	1190:1191	Collisional induced dissociation (CID)-tandem MS and MS3 acquisitions of chemically prepared NAPE allowed to unequivocally recognize the N-linked fatty acyl chain and to establish the diagnostic product ions that were successfully applied to identify NAPE in lipid extracts of yellow lupin seeds.
32840368	7	42	theme	0.13	2072:2075	arg1	±					2070:2070	±	2070:2070	±	2070:2070	The total amount of NAPE in lupin seeds ranged in the interval of 2.00 ± 0.13 mg/g dw, in agreement with other edible legumes.
32840368	1	43	theme	several	447:453	arg1	processes					469:477	several physiological processes	447:477	several physiological processes such as regulation of seed germination and protection against pathogenic attacks	447:558	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines (NAPE) are low abundance phospholipids but important constituents of intracellular membranes of plant tissues, responsible for generating bioactive N-acylethanolamine (NAE), which participates in several physiological processes such as regulation of seed germination and protection against pathogenic attacks.
32840368	1	43	theme	several	447:453	arg1	regulation					487:496	regulation	487:496	regulation of seed germination	487:516	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines (NAPE) are low abundance phospholipids but important constituents of intracellular membranes of plant tissues, responsible for generating bioactive N-acylethanolamine (NAE), which participates in several physiological processes such as regulation of seed germination and protection against pathogenic attacks.
32840368	1	43	theme	several	447:453	arg1	protection					522:531	protection	522:531	protection against pathogenic attacks	522:558	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines (NAPE) are low abundance phospholipids but important constituents of intracellular membranes of plant tissues, responsible for generating bioactive N-acylethanolamine (NAE), which participates in several physiological processes such as regulation of seed germination and protection against pathogenic attacks.
32840368	4	44	theme	seeds	1434:1438	arg1	extracts					1409:1416	lipid extracts	1403:1416	lipid extracts of yellow lupin seeds	1403:1438	Collisional induced dissociation (CID)-tandem MS and MS3 acquisitions of chemically prepared NAPE allowed to unequivocally recognize the N-linked fatty acyl chain and to establish the diagnostic product ions that were successfully applied to identify NAPE in lipid extracts of yellow lupin seeds.
32840368	3	45	theme	electrospray	922:933	arg1	ionization					935:944	electrospray ionization	922:944	electrospray ionization	922:944	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	7	46	theme	2.00	2065:2068	arg1	±					2070:2070	±	2070:2070	±	2070:2070	The total amount of NAPE in lupin seeds ranged in the interval of 2.00 ± 0.13 mg/g dw, in agreement with other edible legumes.
32840368	5	47	theme	sn-1/sn-2	1657:1665	arg1	combinations					1667:1678	several sn-1/sn-2 combinations	1649:1678	several sn-1/sn-2 combinations	1649:1678	The most abundant NAPE species were those containing N-acyl groups C18:1, C18:2; a minor prevalence was found for C16:0, C18:0, and C18:3, and almost the same acyl chains O-linked on the glycerol backbone in several sn-1/sn-2 combinations were observed.
32840368	0	48	theme	Regiochemical	0:12	arg1	Assignment					14:23	Regiochemical Assignment	0:23	Regiochemical Assignment of N-Acylphosphatidylethanolamines (NAPE) by Liquid Chromatography/Electrospray Ionization with Multistage Mass Spectrometry and Its Application to Extracts of Lupin Seeds.	0:196	Regiochemical Assignment of N-Acylphosphatidylethanolamines (NAPE) by Liquid Chromatography/Electrospray Ionization with Multistage Mass Spectrometry and Its Application to Extracts of Lupin Seeds.
32840368	1	49	theme	tissues	353:359	arg1	membranes					334:342	intracellular membranes	320:342	intracellular membranes of plant tissues	320:359	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines (NAPE) are low abundance phospholipids but important constituents of intracellular membranes of plant tissues, responsible for generating bioactive N-acylethanolamine (NAE), which participates in several physiological processes such as regulation of seed germination and protection against pathogenic attacks.
32840368	6	50	theme	isomers	1944:1950	arg1	54:3					1952:1955	two isomers 54:3	1940:1955	two isomers 54:3	1940:1955	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	50	theme	isomers	1944:1950	arg1	m/z					1925:1927	m/z 1006.7837	1925:1937	m/z 1006.7837 (two isomers 54:3)	1925:1956	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	3	51	from	extracts	862:869	arg1	identity					821:828	identity	821:828	identity	821:828	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	3	51	from	extracts	862:869	arg1	occurrence					834:843	occurrence	834:843	occurrence	834:843	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	3	52	dep	FTMS	1086:1089	arg1	i.e.					1071:1074	i.e.	1071:1074	i.e.	1071:1074	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	1	53	theme	responsible	362:372	arg1	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines					198:249	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines	198:249	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines (NAPE)	198:256	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines (NAPE) are low abundance phospholipids but important constituents of intracellular membranes of plant tissues, responsible for generating bioactive N-acylethanolamine (NAE), which participates in several physiological processes such as regulation of seed germination and protection against pathogenic attacks.
32840368	1	53	theme	responsible	362:372	arg1	phospholipids					276:288	low abundance phospholipids	262:288	low abundance phospholipids	262:288	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines (NAPE) are low abundance phospholipids but important constituents of intracellular membranes of plant tissues, responsible for generating bioactive N-acylethanolamine (NAE), which participates in several physiological processes such as regulation of seed germination and protection against pathogenic attacks.
32840368	3	54	theme	ion	958:960	arg1	mode					962:965	negative ion mode	949:965	negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS)	949:1052	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	2	55	theme	analytical	569:578	arg1	point					580:584	an analytical point	566:584	an analytical point of view	566:592	From an analytical point of view, the critical aspect of these bioactive lipids lies in the determination of fatty acyl chains located in sn-1/sn-2 position on the glycerol backbone (O-linked), along with the amide-bound (N-linked) fatty acyl chain.
32840368	6	56	theme	deprotonated	1753:1764	arg1	isomers					1710:1716	The positional isomers	1695:1716	The positional isomers of NAPE species	1695:1732	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	56	theme	deprotonated	1753:1764	arg1	m/z					1891:1893	m/z 1004.7686	1891:1903	m/z 1004.7686 (two isomers 54:4)	1891:1922	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	56	theme	deprotonated	1753:1764	arg1	molecules					1766:1774	deprotonated molecules	1753:1774	deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2)	1753:1996	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	56	theme	deprotonated	1753:1764	arg1	m/z					1925:1927	m/z 1006.7837	1925:1937	m/z 1006.7837 (two isomers 54:3)	1925:1956	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	56	theme	deprotonated	1753:1764	arg1	[M-H					1777:1780	[M-H	1777:1780	[M-H	1777:1780	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	56	theme	deprotonated	1753:1764	arg1	m/z					1823:1825	m/z 980.7694	1823:1834	m/z 980.7694 (two isomers 52:2)	1823:1853	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	56	theme	deprotonated	1753:1764	arg1	m/z					1963:1965	m/z 1008.8026	1963:1975	m/z 1008.8026 (single isomer 54:2)	1963:1996	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	56	theme	deprotonated	1753:1764	arg1	m/z					1856:1858	m/z 1002.7535	1856:1868	m/z 1002.7535 (four isomers 54:5)	1856:1888	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	4	57	theme	N-linked	1281:1288	arg1	chain					1301:1305	the N-linked fatty acyl chain	1277:1305	the N-linked fatty acyl chain	1277:1305	Collisional induced dissociation (CID)-tandem MS and MS3 acquisitions of chemically prepared NAPE allowed to unequivocally recognize the N-linked fatty acyl chain and to establish the diagnostic product ions that were successfully applied to identify NAPE in lipid extracts of yellow lupin seeds.
32840368	5	58	theme	glycerol	1628:1635	arg1	backbone					1637:1644	the glycerol backbone	1624:1644	the glycerol backbone in several sn-1/sn-2 combinations	1624:1678	The most abundant NAPE species were those containing N-acyl groups C18:1, C18:2; a minor prevalence was found for C16:0, C18:0, and C18:3, and almost the same acyl chains O-linked on the glycerol backbone in several sn-1/sn-2 combinations were observed.
32840368	3	59	theme	ion	1108:1110	arg1	trap					1112:1115	linear ion trap	1101:1115	linear ion trap	1101:1115	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	3	59	theme	ion	1108:1110	arg1	LIT					1118:1120	LIT	1118:1120	LIT	1118:1120	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	4	60	theme	acyl	1296:1299	arg1	chain					1301:1305	the N-linked fatty acyl chain	1277:1305	the N-linked fatty acyl chain	1277:1305	Collisional induced dissociation (CID)-tandem MS and MS3 acquisitions of chemically prepared NAPE allowed to unequivocally recognize the N-linked fatty acyl chain and to establish the diagnostic product ions that were successfully applied to identify NAPE in lipid extracts of yellow lupin seeds.
32840368	6	61	dep	molecules	1766:1774	arg1	54:3					1952:1955	two isomers 54:3	1940:1955	two isomers 54:3	1940:1955	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	61	dep	molecules	1766:1774	arg1	m/z					1891:1893	m/z 1004.7686	1891:1903	m/z 1004.7686 (two isomers 54:4)	1891:1922	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	61	dep	molecules	1766:1774	arg1	molecules					1766:1774	deprotonated molecules	1753:1774	deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2)	1753:1996	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	61	dep	molecules	1766:1774	arg1	52:2					1849:1852	two isomers 52:2	1837:1852	two isomers 52:2	1837:1852	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	61	dep	molecules	1766:1774	arg1	m/z					1925:1927	m/z 1006.7837	1925:1937	m/z 1006.7837 (two isomers 54:3)	1925:1956	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	61	dep	molecules	1766:1774	arg1	54:2					1992:1995	single isomer 54:2	1978:1995	single isomer 54:2	1978:1995	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	61	dep	molecules	1766:1774	arg1	54:5					1884:1887	four isomers 54:5	1871:1887	four isomers 54:5	1871:1887	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	61	dep	molecules	1766:1774	arg1	m/z					1823:1825	m/z 980.7694	1823:1834	m/z 980.7694 (two isomers 52:2)	1823:1853	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	61	dep	molecules	1766:1774	arg1	m/z					1963:1965	m/z 1008.8026	1963:1975	m/z 1008.8026 (single isomer 54:2)	1963:1996	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	61	dep	molecules	1766:1774	arg1	54:4					1918:1921	two isomers 54:4	1906:1921	two isomers 54:4	1906:1921	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	61	dep	molecules	1766:1774	arg1	m/z					1856:1858	m/z 1002.7535	1856:1868	m/z 1002.7535 (four isomers 54:5)	1856:1888	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	3	62	theme	liquid	987:992	arg1	RPLC-ESI					1010:1017	RPLC-ESI	1010:1017	RPLC-ESI	1010:1017	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	3	62	theme	liquid	987:992	arg1	chromatography					994:1007	reversed-phase liquid chromatography	972:1007	reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS)	972:1052	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	2	63	from	position	709:716	arg1	located					688:694	located	688:694	located	688:694	From an analytical point of view, the critical aspect of these bioactive lipids lies in the determination of fatty acyl chains located in sn-1/sn-2 position on the glycerol backbone (O-linked), along with the amide-bound (N-linked) fatty acyl chain.
32840368	7	64	dep	dw	2082:2083	arg1	mg/g					2077:2080	mg/g	2077:2080	mg/g	2077:2080	The total amount of NAPE in lupin seeds ranged in the interval of 2.00 ± 0.13 mg/g dw, in agreement with other edible legumes.
32840368	6	65	theme	single	1978:1983	arg1	54:2					1992:1995	single isomer 54:2	1978:1995	single isomer 54:2	1978:1995	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	65	theme	single	1978:1983	arg1	m/z					1963:1965	m/z 1008.8026	1963:1975	m/z 1008.8026 (single isomer 54:2)	1963:1996	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	4	66	theme	diagnostic	1328:1337	arg1	ions					1347:1350	the diagnostic product ions	1324:1350	the diagnostic product ions that were successfully applied to identify NAPE in lipid extracts of yellow lupin seeds	1324:1438	Collisional induced dissociation (CID)-tandem MS and MS3 acquisitions of chemically prepared NAPE allowed to unequivocally recognize the N-linked fatty acyl chain and to establish the diagnostic product ions that were successfully applied to identify NAPE in lipid extracts of yellow lupin seeds.
32840368	1	67	theme	intracellular	320:332	arg1	membranes					334:342	intracellular membranes	320:342	intracellular membranes of plant tissues	320:359	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines (NAPE) are low abundance phospholipids but important constituents of intracellular membranes of plant tissues, responsible for generating bioactive N-acylethanolamine (NAE), which participates in several physiological processes such as regulation of seed germination and protection against pathogenic attacks.
32840368	2	68	theme	chains	681:686	arg1	determination					653:665	the determination	649:665	the determination of fatty acyl chains located in sn-1/sn-2 position on the glycerol backbone (O-linked)	649:752	From an analytical point of view, the critical aspect of these bioactive lipids lies in the determination of fatty acyl chains located in sn-1/sn-2 position on the glycerol backbone (O-linked), along with the amide-bound (N-linked) fatty acyl chain.
32840368	4	69	theme	dissociation	1164:1175	arg1	MS					1190:1191	Collisional induced dissociation (CID)-tandem MS and MS3 acquisitions	1144:1212	MS	1190:1191	Collisional induced dissociation (CID)-tandem MS and MS3 acquisitions of chemically prepared NAPE allowed to unequivocally recognize the N-linked fatty acyl chain and to establish the diagnostic product ions that were successfully applied to identify NAPE in lipid extracts of yellow lupin seeds.
32840368	2	70	theme	fatty	670:674	arg1	chains					681:686	fatty acyl chains	670:686	fatty acyl chains located in sn-1/sn-2 position on the glycerol backbone (O-linked)	670:752	From an analytical point of view, the critical aspect of these bioactive lipids lies in the determination of fatty acyl chains located in sn-1/sn-2 position on the glycerol backbone (O-linked), along with the amide-bound (N-linked) fatty acyl chain.
32840368	8	71	theme	assessment	2168:2177	arg1	method					2179:2184	a robust assessment method	2159:2184	a robust assessment method potentially applicable to biological extracts containing NAPE species	2159:2254	We anticipate our approach to be a robust assessment method potentially applicable to biological extracts containing NAPE species and can provide comprehensive profiles and contents.
32840368	3	72	theme	high-	1064:1068	arg1	resolution/accuracy					1123:1141	high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy	1064:1141	high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy	1064:1141	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	5	73	theme	same	1595:1598	arg1	chains					1605:1610	almost the same acyl chains	1584:1610	almost the same acyl chains O-linked on the glycerol backbone in several sn-1/sn-2 combinations	1584:1678	The most abundant NAPE species were those containing N-acyl groups C18:1, C18:2; a minor prevalence was found for C16:0, C18:0, and C18:3, and almost the same acyl chains O-linked on the glycerol backbone in several sn-1/sn-2 combinations were observed.
32840368	5	74	dep	groups	1501:1506	arg1	C18:2					1515:1519	C18:2	1515:1519	C18:2	1515:1519	The most abundant NAPE species were those containing N-acyl groups C18:1, C18:2; a minor prevalence was found for C16:0, C18:0, and C18:3, and almost the same acyl chains O-linked on the glycerol backbone in several sn-1/sn-2 combinations were observed.
32840368	4	75	from	NAPE	1395:1398	arg1	extracts					1409:1416	lipid extracts	1403:1416	lipid extracts of yellow lupin seeds	1403:1438	Collisional induced dissociation (CID)-tandem MS and MS3 acquisitions of chemically prepared NAPE allowed to unequivocally recognize the N-linked fatty acyl chain and to establish the diagnostic product ions that were successfully applied to identify NAPE in lipid extracts of yellow lupin seeds.
32840368	2	76	theme	lipids	634:639	arg1	aspect					608:613	the critical aspect	595:613	the critical aspect of these bioactive lipids	595:639	From an analytical point of view, the critical aspect of these bioactive lipids lies in the determination of fatty acyl chains located in sn-1/sn-2 position on the glycerol backbone (O-linked), along with the amide-bound (N-linked) fatty acyl chain.
32840368	1	77	theme	abundance	266:274	arg1	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines					198:249	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines	198:249	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines (NAPE)	198:256	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines (NAPE) are low abundance phospholipids but important constituents of intracellular membranes of plant tissues, responsible for generating bioactive N-acylethanolamine (NAE), which participates in several physiological processes such as regulation of seed germination and protection against pathogenic attacks.
32840368	1	77	theme	abundance	266:274	arg1	phospholipids					276:288	low abundance phospholipids	262:288	low abundance phospholipids	262:288	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines (NAPE) are low abundance phospholipids but important constituents of intracellular membranes of plant tissues, responsible for generating bioactive N-acylethanolamine (NAE), which participates in several physiological processes such as regulation of seed germination and protection against pathogenic attacks.
32840368	6	78	theme	species	1726:1732	arg1	isomers					1710:1716	The positional isomers	1695:1716	The positional isomers of NAPE species	1695:1732	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	78	theme	species	1726:1732	arg1	molecules					1766:1774	deprotonated molecules	1753:1774	deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2)	1753:1996	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	0	79	theme	Lupin	185:189	arg1	Seeds					191:195	Lupin Seeds	185:195	Lupin Seeds	185:195	Regiochemical Assignment of N-Acylphosphatidylethanolamines (NAPE) by Liquid Chromatography/Electrospray Ionization with Multistage Mass Spectrometry and Its Application to Extracts of Lupin Seeds.
32840368	8	80	theme	NAPE	2243:2246	arg1	species					2248:2254	NAPE species	2243:2254	NAPE species	2243:2254	We anticipate our approach to be a robust assessment method potentially applicable to biological extracts containing NAPE species and can provide comprehensive profiles and contents.
32840368	1	81	theme	seed	501:504	arg1	germination					506:516	seed germination	501:516	seed germination	501:516	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines (NAPE) are low abundance phospholipids but important constituents of intracellular membranes of plant tissues, responsible for generating bioactive N-acylethanolamine (NAE), which participates in several physiological processes such as regulation of seed germination and protection against pathogenic attacks.
32840368	7	82	with	agreement	2089:2097	arg1	legumes					2117:2123	other edible legumes	2104:2123	other edible legumes	2104:2123	The total amount of NAPE in lupin seeds ranged in the interval of 2.00 ± 0.13 mg/g dw, in agreement with other edible legumes.
32840368	4	83	theme	induced	1156:1162	arg1	MS					1190:1191	Collisional induced dissociation (CID)-tandem MS and MS3 acquisitions	1144:1212	MS	1190:1191	Collisional induced dissociation (CID)-tandem MS and MS3 acquisitions of chemically prepared NAPE allowed to unequivocally recognize the N-linked fatty acyl chain and to establish the diagnostic product ions that were successfully applied to identify NAPE in lipid extracts of yellow lupin seeds.
32840368	3	84	theme	lipid	856:860	arg1	extracts					862:869	lipid extracts	856:869	lipid extracts of lupin seeds (Lupinus luteus L.)	856:904	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	0	85	theme	Chromatography/Electrospray	77:103	arg1	Spectrometry					137:148	Liquid Chromatography/Electrospray Ionization with Multistage Mass Spectrometry	70:148	Liquid Chromatography/Electrospray Ionization with Multistage Mass Spectrometry	70:148	Regiochemical Assignment of N-Acylphosphatidylethanolamines (NAPE) by Liquid Chromatography/Electrospray Ionization with Multistage Mass Spectrometry and Its Application to Extracts of Lupin Seeds.
32840368	3	86	dep	mode	962:965	arg1	either					1054:1059	either	1054:1059	either	1054:1059	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	2	87	theme	view	589:592	arg1	point					580:584	an analytical point	566:584	an analytical point of view	566:592	From an analytical point of view, the critical aspect of these bioactive lipids lies in the determination of fatty acyl chains located in sn-1/sn-2 position on the glycerol backbone (O-linked), along with the amide-bound (N-linked) fatty acyl chain.
32840368	6	88	theme	NAPE	1721:1724	arg1	species					1726:1732	NAPE species	1721:1732	NAPE species	1721:1732	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	3	89	theme	NAPE	848:851	arg1	identity					821:828	identity	821:828	identity	821:828	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	3	89	theme	NAPE	848:851	arg1	occurrence					834:843	occurrence	834:843	occurrence	834:843	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	0	90	theme	with	116:119	arg1	Spectrometry					137:148	Liquid Chromatography/Electrospray Ionization with Multistage Mass Spectrometry	70:148	Liquid Chromatography/Electrospray Ionization with Multistage Mass Spectrometry	70:148	Regiochemical Assignment of N-Acylphosphatidylethanolamines (NAPE) by Liquid Chromatography/Electrospray Ionization with Multistage Mass Spectrometry and Its Application to Extracts of Lupin Seeds.
32840368	3	91	theme	Orbitrap	1077:1084	arg1	FTMS					1086:1089	Orbitrap FTMS	1077:1089	Orbitrap FTMS	1077:1089	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	7	92	from	amount	2009:2014	arg1	seeds					2033:2037	lupin seeds	2027:2037	lupin seeds	2027:2037	The total amount of NAPE in lupin seeds ranged in the interval of 2.00 ± 0.13 mg/g dw, in agreement with other edible legumes.
32840368	2	93	theme	acyl	799:802	arg1	chain					804:808	the amide-bound (N-linked) fatty acyl chain	766:808	the amide-bound (N-linked) fatty acyl chain	766:808	From an analytical point of view, the critical aspect of these bioactive lipids lies in the determination of fatty acyl chains located in sn-1/sn-2 position on the glycerol backbone (O-linked), along with the amide-bound (N-linked) fatty acyl chain.
32840368	7	94	theme	edible	2110:2115	arg1	legumes					2117:2123	other edible legumes	2104:2123	other edible legumes	2104:2123	The total amount of NAPE in lupin seeds ranged in the interval of 2.00 ± 0.13 mg/g dw, in agreement with other edible legumes.
32840368	3	95	from	identity	821:828	arg1	extracts					862:869	lipid extracts	856:869	lipid extracts of lupin seeds (Lupinus luteus L.)	856:904	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	4	96	theme	prepared	1228:1235	arg1	NAPE					1237:1240	chemically prepared NAPE	1217:1240	chemically prepared NAPE	1217:1240	Collisional induced dissociation (CID)-tandem MS and MS3 acquisitions of chemically prepared NAPE allowed to unequivocally recognize the N-linked fatty acyl chain and to establish the diagnostic product ions that were successfully applied to identify NAPE in lipid extracts of yellow lupin seeds.
32840368	6	97	theme	isomers	1841:1847	arg1	52:2					1849:1852	two isomers 52:2	1837:1852	two isomers 52:2	1837:1852	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	97	theme	isomers	1841:1847	arg1	m/z					1823:1825	m/z 980.7694	1823:1834	m/z 980.7694 (two isomers 52:2)	1823:1853	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	3	98	theme	seeds	880:884	arg1	extracts					862:869	lipid extracts	856:869	lipid extracts of lupin seeds (Lupinus luteus L.)	856:904	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	0	99	theme	Mass	132:135	arg1	Spectrometry					137:148	Liquid Chromatography/Electrospray Ionization with Multistage Mass Spectrometry	70:148	Liquid Chromatography/Electrospray Ionization with Multistage Mass Spectrometry	70:148	Regiochemical Assignment of N-Acylphosphatidylethanolamines (NAPE) by Liquid Chromatography/Electrospray Ionization with Multistage Mass Spectrometry and Its Application to Extracts of Lupin Seeds.
32840368	7	100	theme	lupin	2027:2031	arg1	seeds					2033:2037	lupin seeds	2027:2037	lupin seeds	2027:2037	The total amount of NAPE in lupin seeds ranged in the interval of 2.00 ± 0.13 mg/g dw, in agreement with other edible legumes.
32840368	3	101	dep	Lupinus	887:893	arg1	L.					902:903	Lupinus luteus L.	887:903	Lupinus luteus L.	887:903	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	2	102	link	N-linked	783:790	arg1	chain					804:808	the amide-bound (N-linked) fatty acyl chain	766:808	the amide-bound (N-linked) fatty acyl chain	766:808	From an analytical point of view, the critical aspect of these bioactive lipids lies in the determination of fatty acyl chains located in sn-1/sn-2 position on the glycerol backbone (O-linked), along with the amide-bound (N-linked) fatty acyl chain.
32840368	3	103	from	occurrence	834:843	arg1	extracts					862:869	lipid extracts	856:869	lipid extracts of lupin seeds (Lupinus luteus L.)	856:904	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	2	104	theme	glycerol	725:732	arg1	backbone					734:741	the glycerol backbone	721:741	the glycerol backbone	721:741	From an analytical point of view, the critical aspect of these bioactive lipids lies in the determination of fatty acyl chains located in sn-1/sn-2 position on the glycerol backbone (O-linked), along with the amide-bound (N-linked) fatty acyl chain.
32840368	4	105	theme	yellow	1421:1426	arg1	seeds					1434:1438	yellow lupin seeds	1421:1438	yellow lupin seeds	1421:1438	Collisional induced dissociation (CID)-tandem MS and MS3 acquisitions of chemically prepared NAPE allowed to unequivocally recognize the N-linked fatty acyl chain and to establish the diagnostic product ions that were successfully applied to identify NAPE in lipid extracts of yellow lupin seeds.
32840368	3	106	dep	identity	821:828	arg1	the					817:819	the	817:819	the	817:819	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	3	107	theme	low-	1095:1098	arg1	resolution/accuracy					1123:1141	high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy	1064:1141	high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy	1064:1141	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	3	108	theme	negative	949:956	arg1	mode					962:965	negative ion mode	949:965	negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS)	949:1052	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	5	109	theme	NAPE	1459:1462	arg1	species					1464:1470	The most abundant NAPE species	1441:1470	The most abundant NAPE species	1441:1470	The most abundant NAPE species were those containing N-acyl groups C18:1, C18:2; a minor prevalence was found for C16:0, C18:0, and C18:3, and almost the same acyl chains O-linked on the glycerol backbone in several sn-1/sn-2 combinations were observed.
32840368	5	109	theme	NAPE	1459:1462	arg1	those					1477:1481	those	1477:1481	those	1477:1481	The most abundant NAPE species were those containing N-acyl groups C18:1, C18:2; a minor prevalence was found for C16:0, C18:0, and C18:3, and almost the same acyl chains O-linked on the glycerol backbone in several sn-1/sn-2 combinations were observed.
32840368	4	110	theme	MS3	1197:1199	arg1	acquisitions					1201:1212	Collisional induced dissociation (CID)-tandem MS and MS3 acquisitions	1144:1212	acquisitions	1201:1212	Collisional induced dissociation (CID)-tandem MS and MS3 acquisitions of chemically prepared NAPE allowed to unequivocally recognize the N-linked fatty acyl chain and to establish the diagnostic product ions that were successfully applied to identify NAPE in lipid extracts of yellow lupin seeds.
32840368	5	111	theme	several	1649:1655	arg1	combinations					1667:1678	several sn-1/sn-2 combinations	1649:1678	several sn-1/sn-2 combinations	1649:1678	The most abundant NAPE species were those containing N-acyl groups C18:1, C18:2; a minor prevalence was found for C16:0, C18:0, and C18:3, and almost the same acyl chains O-linked on the glycerol backbone in several sn-1/sn-2 combinations were observed.
32840368	1	112	theme	physiological	455:467	arg1	processes					469:477	several physiological processes	447:477	several physiological processes such as regulation of seed germination and protection against pathogenic attacks	447:558	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines (NAPE) are low abundance phospholipids but important constituents of intracellular membranes of plant tissues, responsible for generating bioactive N-acylethanolamine (NAE), which participates in several physiological processes such as regulation of seed germination and protection against pathogenic attacks.
32840368	1	112	theme	physiological	455:467	arg1	regulation					487:496	regulation	487:496	regulation of seed germination	487:516	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines (NAPE) are low abundance phospholipids but important constituents of intracellular membranes of plant tissues, responsible for generating bioactive N-acylethanolamine (NAE), which participates in several physiological processes such as regulation of seed germination and protection against pathogenic attacks.
32840368	1	112	theme	physiological	455:467	arg1	protection					522:531	protection	522:531	protection against pathogenic attacks	522:558	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines (NAPE) are low abundance phospholipids but important constituents of intracellular membranes of plant tissues, responsible for generating bioactive N-acylethanolamine (NAE), which participates in several physiological processes such as regulation of seed germination and protection against pathogenic attacks.
32840368	0	113	theme	N-Acylphosphatidylethanolamines	28:58	arg1	Assignment					14:23	Regiochemical Assignment	0:23	Regiochemical Assignment of N-Acylphosphatidylethanolamines (NAPE) by Liquid Chromatography/Electrospray Ionization with Multistage Mass Spectrometry and Its Application to Extracts of Lupin Seeds.	0:196	Regiochemical Assignment of N-Acylphosphatidylethanolamines (NAPE) by Liquid Chromatography/Electrospray Ionization with Multistage Mass Spectrometry and Its Application to Extracts of Lupin Seeds.
32840368	3	114	theme	reversed-phase	972:985	arg1	RPLC-ESI					1010:1017	RPLC-ESI	1010:1017	RPLC-ESI	1010:1017	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	3	114	theme	reversed-phase	972:985	arg1	chromatography					994:1007	reversed-phase liquid chromatography	972:1007	reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS)	972:1052	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	2	115	from	located	688:694	arg1	position					709:716	sn-1/sn-2 position	699:716	sn-1/sn-2 position	699:716	From an analytical point of view, the critical aspect of these bioactive lipids lies in the determination of fatty acyl chains located in sn-1/sn-2 position on the glycerol backbone (O-linked), along with the amide-bound (N-linked) fatty acyl chain.
32840368	5	116	theme	minor	1524:1528	arg1	prevalence					1530:1539	a minor prevalence	1522:1539	a minor prevalence	1522:1539	The most abundant NAPE species were those containing N-acyl groups C18:1, C18:2; a minor prevalence was found for C16:0, C18:0, and C18:3, and almost the same acyl chains O-linked on the glycerol backbone in several sn-1/sn-2 combinations were observed.
32840368	4	117	theme	fatty	1290:1294	arg1	chain					1301:1305	the N-linked fatty acyl chain	1277:1305	the N-linked fatty acyl chain	1277:1305	Collisional induced dissociation (CID)-tandem MS and MS3 acquisitions of chemically prepared NAPE allowed to unequivocally recognize the N-linked fatty acyl chain and to establish the diagnostic product ions that were successfully applied to identify NAPE in lipid extracts of yellow lupin seeds.
32840368	6	118	theme	isomers	1876:1882	arg1	54:5					1884:1887	four isomers 54:5	1871:1887	four isomers 54:5	1871:1887	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	118	theme	isomers	1876:1882	arg1	m/z					1856:1858	m/z 1002.7535	1856:1868	m/z 1002.7535 (four isomers 54:5)	1856:1888	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	3	119	theme	linear	1101:1106	arg1	trap					1112:1115	linear ion trap	1101:1115	linear ion trap	1101:1115	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	3	119	theme	linear	1101:1106	arg1	LIT					1118:1120	LIT	1118:1120	LIT	1118:1120	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	8	120	contain	containing	2232:2241	arg1	extracts					2223:2230	biological extracts	2212:2230	biological extracts containing NAPE species	2212:2254	We anticipate our approach to be a robust assessment method potentially applicable to biological extracts containing NAPE species and can provide comprehensive profiles and contents.
32840368	8	120	contain	containing	2232:2241	arg2	species					2248:2254	NAPE species	2243:2254	NAPE species	2243:2254	We anticipate our approach to be a robust assessment method potentially applicable to biological extracts containing NAPE species and can provide comprehensive profiles and contents.
32840368	1	121	theme	bioactive	389:397	arg1	NAE					419:421	NAE	419:421	NAE	419:421	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines (NAPE) are low abundance phospholipids but important constituents of intracellular membranes of plant tissues, responsible for generating bioactive N-acylethanolamine (NAE), which participates in several physiological processes such as regulation of seed germination and protection against pathogenic attacks.
32840368	1	121	theme	bioactive	389:397	arg1	N-acylethanolamine					399:416	bioactive N-acylethanolamine	389:416	bioactive N-acylethanolamine (NAE)	389:422	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines (NAPE) are low abundance phospholipids but important constituents of intracellular membranes of plant tissues, responsible for generating bioactive N-acylethanolamine (NAE), which participates in several physiological processes such as regulation of seed germination and protection against pathogenic attacks.
32840368	5	122	from	backbone	1637:1644	arg1	combinations					1667:1678	several sn-1/sn-2 combinations	1649:1678	several sn-1/sn-2 combinations	1649:1678	The most abundant NAPE species were those containing N-acyl groups C18:1, C18:2; a minor prevalence was found for C16:0, C18:0, and C18:3, and almost the same acyl chains O-linked on the glycerol backbone in several sn-1/sn-2 combinations were observed.
32840368	3	123	from	NAPE	848:851	arg1	extracts					862:869	lipid extracts	856:869	lipid extracts of lupin seeds (Lupinus luteus L.)	856:904	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	7	124	dep	mg/g	2077:2080	arg1	±					2070:2070	±	2070:2070	±	2070:2070	The total amount of NAPE in lupin seeds ranged in the interval of 2.00 ± 0.13 mg/g dw, in agreement with other edible legumes.
32840368	7	125	theme	NAPE	2019:2022	arg1	NAPE					2019:2022	NAPE	2019:2022	NAPE	2019:2022	The total amount of NAPE in lupin seeds ranged in the interval of 2.00 ± 0.13 mg/g dw, in agreement with other edible legumes.
32840368	7	125	theme	NAPE	2019:2022	arg1	amount					2009:2014	The total amount	1999:2014	The total amount of NAPE in lupin seeds	1999:2037	The total amount of NAPE in lupin seeds ranged in the interval of 2.00 ± 0.13 mg/g dw, in agreement with other edible legumes.
32840368	6	126	theme	isomer	1985:1990	arg1	54:2					1992:1995	single isomer 54:2	1978:1995	single isomer 54:2	1978:1995	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	126	theme	isomer	1985:1990	arg1	m/z					1963:1965	m/z 1008.8026	1963:1975	m/z 1008.8026 (single isomer 54:2)	1963:1996	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	5	127	link	O-linked	1612:1619	arg1	chains					1605:1610	almost the same acyl chains	1584:1610	almost the same acyl chains O-linked on the glycerol backbone in several sn-1/sn-2 combinations	1584:1678	The most abundant NAPE species were those containing N-acyl groups C18:1, C18:2; a minor prevalence was found for C16:0, C18:0, and C18:3, and almost the same acyl chains O-linked on the glycerol backbone in several sn-1/sn-2 combinations were observed.
32840368	1	128	theme	important	294:302	arg1	constituents					304:315	important constituents	294:315	important constituents of intracellular membranes of plant tissues	294:359	1,2-Diacyl-sn-glycero-3-phospho-N-acyl-ethanolamines (NAPE) are low abundance phospholipids but important constituents of intracellular membranes of plant tissues, responsible for generating bioactive N-acylethanolamine (NAE), which participates in several physiological processes such as regulation of seed germination and protection against pathogenic attacks.
32840368	3	129	theme	mass	1031:1034	arg1	MS					1050:1051	MS	1050:1051	MS	1050:1051	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	3	129	theme	mass	1031:1034	arg1	spectrometry					1036:1047	mass spectrometry	1031:1047	mass spectrometry (MS)	1031:1052	Here, the identity and occurrence of NAPE in lipid extracts of lupin seeds (Lupinus luteus L.) was assessed by electrospray ionization in negative ion mode upon reversed-phase liquid chromatography (RPLC-ESI) coupled to mass spectrometry (MS) either at high- (i.e., Orbitrap FTMS) or low- (linear ion trap, LIT) resolution/accuracy.
32840368	4	130	theme	product	1339:1345	arg1	ions					1347:1350	the diagnostic product ions	1324:1350	the diagnostic product ions that were successfully applied to identify NAPE in lipid extracts of yellow lupin seeds	1324:1438	Collisional induced dissociation (CID)-tandem MS and MS3 acquisitions of chemically prepared NAPE allowed to unequivocally recognize the N-linked fatty acyl chain and to establish the diagnostic product ions that were successfully applied to identify NAPE in lipid extracts of yellow lupin seeds.
32840368	6	131	theme	isomers	1910:1916	arg1	54:4					1918:1921	two isomers 54:4	1906:1921	two isomers 54:4	1906:1921	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	6	131	theme	isomers	1910:1916	arg1	m/z					1891:1893	m/z 1004.7686	1891:1903	m/z 1004.7686 (two isomers 54:4)	1891:1922	The positional isomers of NAPE species were identified as deprotonated molecules ([M-H]-) at m/z 978.7541 (three isomers 52:3), m/z 980.7694 (two isomers 52:2), m/z 1002.7535 (four isomers 54:5), m/z 1004.7686 (two isomers 54:4), m/z 1006.7837 (two isomers 54:3), and m/z 1008.8026 (single isomer 54:2).
32840368	4	132	link	N-linked	1281:1288	arg1	chain					1301:1305	the N-linked fatty acyl chain	1277:1305	the N-linked fatty acyl chain	1277:1305	Collisional induced dissociation (CID)-tandem MS and MS3 acquisitions of chemically prepared NAPE allowed to unequivocally recognize the N-linked fatty acyl chain and to establish the diagnostic product ions that were successfully applied to identify NAPE in lipid extracts of yellow lupin seeds.
32230742	10	0	theme	studies	1372:1378	arg1	results					1352:1358	The results	1348:1358	The results of in vitro studies along with physicochemical characterizations	1348:1423	The results of in vitro studies along with physicochemical characterizations reflect the great potentials of the produced samples as scaffolds for application in skin tissue engineering.
32230742	1	1	theme	made-on-purpose	162:176	arg1	scaffolds					189:197	made-on-purpose biomimetic scaffolds	162:197	made-on-purpose biomimetic scaffolds	162:197	Electrospinning is a versatile technique for fabrication of made-on-purpose biomimetic scaffolds.
32230742	10	2	theme	skin	1510:1513	arg1	engineering					1522:1532	skin tissue engineering	1510:1532	skin tissue engineering	1510:1532	The results of in vitro studies along with physicochemical characterizations reflect the great potentials of the produced samples as scaffolds for application in skin tissue engineering.
32230742	7	3	theme	biocompatible	959:971	arg1	scaffolds					944:952	the scaffolds	940:952	the scaffolds more biocompatible	940:971	Furthermore, grafting the surface with the collagen/chitosan blend rendered the scaffolds more biocompatible with improved attachment and spreading of keratinocyte cell lines (HaCaT).
32230742	1	4	theme	biomimetic	178:187	arg1	scaffolds					189:197	made-on-purpose biomimetic scaffolds	162:197	made-on-purpose biomimetic scaffolds	162:197	Electrospinning is a versatile technique for fabrication of made-on-purpose biomimetic scaffolds.
32230742	4	5	dep	in	543:544	arg1	vitro					546:550	vitro	546:550	vitro	546:550	Physicochemical characterizations as well as in vitro evaluations were conducted using different methods.
32230742	9	6	theme	coated	1334:1339	arg1	blend					1341:1345	the coated blend	1330:1345	the coated blend	1330:1345	Antibacterial assay with Staphylococcus aureus as Gram-positive and Escherichia coli as Gram-negative species corroborated the bactericidal effects of chitosan utilized in the composition of the coated blend.
32230742	10	7	theme	samples	1470:1476	arg1	scaffolds					1481:1489	scaffolds	1481:1489	scaffolds for application in skin tissue engineering	1481:1532	The results of in vitro studies along with physicochemical characterizations reflect the great potentials of the produced samples as scaffolds for application in skin tissue engineering.
32230742	10	7	theme	samples	1470:1476	arg1	potentials					1443:1452	the great potentials	1433:1452	the great potentials of the produced samples	1433:1476	The results of in vitro studies along with physicochemical characterizations reflect the great potentials of the produced samples as scaffolds for application in skin tissue engineering.
32230742	2	8	theme	selective	375:383	arg1	removal					385:391	the selective removal	371:391	the selective removal of PVP from the PCL/PVP mesh	371:420	In this study, optimized electrospun fibrous membranes were produced by simultaneous electrospinning of polycaprolactone (PCL) and polyvinylpyrrolidone (PVP), followed by the selective removal of PVP from the PCL/PVP mesh.
32230742	10	9	dep	along	1380:1384	arg1	with					1386:1389	with	1386:1389	with	1386:1389	The results of in vitro studies along with physicochemical characterizations reflect the great potentials of the produced samples as scaffolds for application in skin tissue engineering.
32230742	7	10	with	surface	890:896	arg1	blend					925:929	the collagen/chitosan blend	903:929	the collagen/chitosan blend	903:929	Furthermore, grafting the surface with the collagen/chitosan blend rendered the scaffolds more biocompatible with improved attachment and spreading of keratinocyte cell lines (HaCaT).
32230742	10	11	theme	tissue	1515:1520	arg1	engineering					1522:1532	skin tissue engineering	1510:1532	skin tissue engineering	1510:1532	The results of in vitro studies along with physicochemical characterizations reflect the great potentials of the produced samples as scaffolds for application in skin tissue engineering.
32230742	9	12	theme	Antibacterial	1139:1151	arg1	assay					1153:1157	Antibacterial assay	1139:1157	Antibacterial assay with Staphylococcus aureus as Gram-positive and Escherichia coli as Gram-negative species	1139:1247	Antibacterial assay with Staphylococcus aureus as Gram-positive and Escherichia coli as Gram-negative species corroborated the bactericidal effects of chitosan utilized in the composition of the coated blend.
32230742	6	13	theme	pore	736:739	arg1	size					741:744	the proper pore size	725:744	the proper pore size obtained after removal of pvp (from 4.46 μm before immersion in water to 33.55 μm after immersion in water for 24 h)	725:861	It seems that the positive trend of cell ingress originates from the proper pore size obtained after removal of pvp (from 4.46 μm before immersion in water to 33.55 μm after immersion in water for 24 h).
32230742	1	14	theme	scaffolds	189:197	arg1	fabrication					147:157	fabrication	147:157	fabrication of made-on-purpose biomimetic scaffolds	147:197	Electrospinning is a versatile technique for fabrication of made-on-purpose biomimetic scaffolds.
32230742	4	15	theme	Physicochemical	498:512	arg1	characterizations					514:530	Physicochemical characterizations	498:530	Physicochemical characterizations as well as in vitro evaluations	498:562	Physicochemical characterizations as well as in vitro evaluations were conducted using different methods.
32230742	8	16	theme	Viability	1048:1056	arg1	evaluation					1058:1067	Viability evaluation	1048:1067	Viability evaluation through MTT assay for HDF cells	1048:1099	Viability evaluation through MTT assay for HDF cells did not reveal any cytotoxic effects.
32230742	10	17	theme	produced	1461:1468	arg1	samples					1470:1476	the produced samples	1457:1476	the produced samples	1457:1476	The results of in vitro studies along with physicochemical characterizations reflect the great potentials of the produced samples as scaffolds for application in skin tissue engineering.
32230742	2	18	theme	polycaprolactone	304:319	arg1	electrospinning					285:299	simultaneous electrospinning	272:299	simultaneous electrospinning of polycaprolactone (PCL) and polyvinylpyrrolidone (PVP)	272:356	In this study, optimized electrospun fibrous membranes were produced by simultaneous electrospinning of polycaprolactone (PCL) and polyvinylpyrrolidone (PVP), followed by the selective removal of PVP from the PCL/PVP mesh.
32230742	6	19	theme	proper	729:734	arg1	size					741:744	the proper pore size	725:744	the proper pore size obtained after removal of pvp (from 4.46 μm before immersion in water to 33.55 μm after immersion in water for 24 h)	725:861	It seems that the positive trend of cell ingress originates from the proper pore size obtained after removal of pvp (from 4.46 μm before immersion in water to 33.55 μm after immersion in water for 24 h).
32230742	8	20	theme	MTT	1077:1079	arg1	assay					1081:1085	MTT assay	1077:1085	MTT assay	1077:1085	Viability evaluation through MTT assay for HDF cells did not reveal any cytotoxic effects.
32230742	5	21	theme	Successful	604:613	arg1	infiltration					620:631	Successful cell infiltration	604:631	Successful cell infiltration into samples	604:644	Successful cell infiltration into samples was observed.
32230742	9	22	theme	blend	1341:1345	arg1	composition					1315:1325	the composition	1311:1325	the composition of the coated blend	1311:1345	Antibacterial assay with Staphylococcus aureus as Gram-positive and Escherichia coli as Gram-negative species corroborated the bactericidal effects of chitosan utilized in the composition of the coated blend.
32230742	0	23	theme	Tailored	0:7	arg1	PCL					9:11	Tailored PCL	0:11	Tailored PCL	0:11	Tailored PCL Scaffolds as Skin Substitutes Using Sacrificial PVP Fibers and Collagen/Chitosan Blends.
32230742	5	24	theme	cell	615:618	arg1	infiltration					620:631	Successful cell infiltration	604:631	Successful cell infiltration into samples	604:644	Successful cell infiltration into samples was observed.
32230742	2	25	theme	PVP	396:398	arg1	removal					385:391	the selective removal	371:391	the selective removal of PVP from the PCL/PVP mesh	371:420	In this study, optimized electrospun fibrous membranes were produced by simultaneous electrospinning of polycaprolactone (PCL) and polyvinylpyrrolidone (PVP), followed by the selective removal of PVP from the PCL/PVP mesh.
32230742	7	26	theme	collagen/chitosan	907:923	arg1	blend					925:929	the collagen/chitosan blend	903:929	the collagen/chitosan blend	903:929	Furthermore, grafting the surface with the collagen/chitosan blend rendered the scaffolds more biocompatible with improved attachment and spreading of keratinocyte cell lines (HaCaT).
32230742	10	27	theme	characterizations	1407:1423	arg1	results					1352:1358	The results	1348:1358	The results of in vitro studies along with physicochemical characterizations	1348:1423	The results of in vitro studies along with physicochemical characterizations reflect the great potentials of the produced samples as scaffolds for application in skin tissue engineering.
32230742	2	28	theme	polyvinylpyrrolidone	331:350	arg1	electrospinning					285:299	simultaneous electrospinning	272:299	simultaneous electrospinning of polycaprolactone (PCL) and polyvinylpyrrolidone (PVP)	272:356	In this study, optimized electrospun fibrous membranes were produced by simultaneous electrospinning of polycaprolactone (PCL) and polyvinylpyrrolidone (PVP), followed by the selective removal of PVP from the PCL/PVP mesh.
32230742	10	29	theme	great	1437:1441	arg1	scaffolds					1481:1489	scaffolds	1481:1489	scaffolds for application in skin tissue engineering	1481:1532	The results of in vitro studies along with physicochemical characterizations reflect the great potentials of the produced samples as scaffolds for application in skin tissue engineering.
32230742	10	29	theme	great	1437:1441	arg1	potentials					1443:1452	the great potentials	1433:1452	the great potentials of the produced samples	1433:1476	The results of in vitro studies along with physicochemical characterizations reflect the great potentials of the produced samples as scaffolds for application in skin tissue engineering.
32230742	9	30	theme	Gram-negative	1227:1239	arg1	species					1241:1247	Gram-negative species	1227:1247	Gram-negative species	1227:1247	Antibacterial assay with Staphylococcus aureus as Gram-positive and Escherichia coli as Gram-negative species corroborated the bactericidal effects of chitosan utilized in the composition of the coated blend.
32230742	6	31	from	removal	761:767	arg1	μm					787:788	4.46 μm	782:788	4.46 μm before immersion in water to 33.55 μm after immersion in water for 24 h	782:860	It seems that the positive trend of cell ingress originates from the proper pore size obtained after removal of pvp (from 4.46 μm before immersion in water to 33.55 μm after immersion in water for 24 h).
32230742	6	32	theme	ingress	701:707	arg1	trend					687:691	the positive trend	674:691	the positive trend of cell ingress	674:707	It seems that the positive trend of cell ingress originates from the proper pore size obtained after removal of pvp (from 4.46 μm before immersion in water to 33.55 μm after immersion in water for 24 h).
32230742	6	33	from	μm	787:788	arg1	removal					761:767	removal	761:767	removal of pvp (from 4.46 μm before immersion in water to 33.55 μm after immersion in water for 24 h)	761:861	It seems that the positive trend of cell ingress originates from the proper pore size obtained after removal of pvp (from 4.46 μm before immersion in water to 33.55 μm after immersion in water for 24 h).
32230742	6	33	from	μm	787:788	arg1	pvp					772:774	pvp	772:774	pvp (from 4.46 μm before immersion in water to 33.55 μm after immersion in water for 24 h)	772:861	It seems that the positive trend of cell ingress originates from the proper pore size obtained after removal of pvp (from 4.46 μm before immersion in water to 33.55 μm after immersion in water for 24 h).
32230742	6	34	from	immersion	834:842	arg1	water					847:851	water	847:851	water for 24 h	847:860	It seems that the positive trend of cell ingress originates from the proper pore size obtained after removal of pvp (from 4.46 μm before immersion in water to 33.55 μm after immersion in water for 24 h).
32230742	4	35	theme	different	585:593	arg1	methods					595:601	different methods	585:601	different methods	585:601	Physicochemical characterizations as well as in vitro evaluations were conducted using different methods.
32230742	2	36	dep	produced	260:267	arg1	followed					359:366	followed	359:366	followed by the selective removal of PVP from the PCL/PVP mesh	359:420	In this study, optimized electrospun fibrous membranes were produced by simultaneous electrospinning of polycaprolactone (PCL) and polyvinylpyrrolidone (PVP), followed by the selective removal of PVP from the PCL/PVP mesh.
32230742	6	37	theme	cell	696:699	arg1	ingress					701:707	cell ingress	696:707	cell ingress	696:707	It seems that the positive trend of cell ingress originates from the proper pore size obtained after removal of pvp (from 4.46 μm before immersion in water to 33.55 μm after immersion in water for 24 h).
32230742	3	38	theme	collagen/chitosan	452:468	arg1	blend					443:447	a blend	441:447	a blend of collagen/chitosan	441:468	After aminolysis, a blend of collagen/chitosan was grafted on the surface.
32230742	2	39	theme	PCL/PVP	409:415	arg1	mesh					417:420	the PCL/PVP mesh	405:420	the PCL/PVP mesh	405:420	In this study, optimized electrospun fibrous membranes were produced by simultaneous electrospinning of polycaprolactone (PCL) and polyvinylpyrrolidone (PVP), followed by the selective removal of PVP from the PCL/PVP mesh.
32230742	8	40	theme	HDF	1091:1093	arg1	cells					1095:1099	HDF cells	1091:1099	HDF cells	1091:1099	Viability evaluation through MTT assay for HDF cells did not reveal any cytotoxic effects.
32230742	9	41	theme	bactericidal	1266:1277	arg1	effects					1279:1285	the bactericidal effects	1262:1285	the bactericidal effects of chitosan utilized in the composition of the coated blend	1262:1345	Antibacterial assay with Staphylococcus aureus as Gram-positive and Escherichia coli as Gram-negative species corroborated the bactericidal effects of chitosan utilized in the composition of the coated blend.
32230742	7	42	theme	cell	1028:1031	arg1	HaCaT					1040:1044	HaCaT	1040:1044	HaCaT	1040:1044	Furthermore, grafting the surface with the collagen/chitosan blend rendered the scaffolds more biocompatible with improved attachment and spreading of keratinocyte cell lines (HaCaT).
32230742	7	42	theme	cell	1028:1031	arg1	lines					1033:1037	keratinocyte cell lines	1015:1037	keratinocyte cell lines (HaCaT)	1015:1045	Furthermore, grafting the surface with the collagen/chitosan blend rendered the scaffolds more biocompatible with improved attachment and spreading of keratinocyte cell lines (HaCaT).
32230742	6	43	from	immersion	797:805	arg1	water					810:814	water	810:814	water	810:814	It seems that the positive trend of cell ingress originates from the proper pore size obtained after removal of pvp (from 4.46 μm before immersion in water to 33.55 μm after immersion in water for 24 h).
32230742	0	44	theme	Sacrificial	49:59	arg1	Fibers					65:70	Sacrificial PVP Fibers	49:70	Sacrificial PVP Fibers	49:70	Tailored PCL Scaffolds as Skin Substitutes Using Sacrificial PVP Fibers and Collagen/Chitosan Blends.
32230742	4	45	theme	in	543:544	arg1	evaluations					552:562	in vitro evaluations	543:562	Physicochemical characterizations as well as in vitro evaluations	498:562	Physicochemical characterizations as well as in vitro evaluations were conducted using different methods.
32230742	8	46	theme	cytotoxic	1120:1128	arg1	effects					1130:1136	any cytotoxic effects	1116:1136	any cytotoxic effects	1116:1136	Viability evaluation through MTT assay for HDF cells did not reveal any cytotoxic effects.
32230742	7	47	theme	lines	1033:1037	arg1	spreading					1002:1010	spreading	1002:1010	spreading	1002:1010	Furthermore, grafting the surface with the collagen/chitosan blend rendered the scaffolds more biocompatible with improved attachment and spreading of keratinocyte cell lines (HaCaT).
32230742	7	47	theme	lines	1033:1037	arg1	attachment					987:996	improved attachment	978:996	improved attachment	978:996	Furthermore, grafting the surface with the collagen/chitosan blend rendered the scaffolds more biocompatible with improved attachment and spreading of keratinocyte cell lines (HaCaT).
32230742	2	48	theme	electrospun	225:235	arg1	membranes					245:253	optimized electrospun fibrous membranes	215:253	optimized electrospun fibrous membranes	215:253	In this study, optimized electrospun fibrous membranes were produced by simultaneous electrospinning of polycaprolactone (PCL) and polyvinylpyrrolidone (PVP), followed by the selective removal of PVP from the PCL/PVP mesh.
32230742	2	49	theme	fibrous	237:243	arg1	membranes					245:253	optimized electrospun fibrous membranes	215:253	optimized electrospun fibrous membranes	215:253	In this study, optimized electrospun fibrous membranes were produced by simultaneous electrospinning of polycaprolactone (PCL) and polyvinylpyrrolidone (PVP), followed by the selective removal of PVP from the PCL/PVP mesh.
32230742	1	50	theme	versatile	123:131	arg1	technique					133:141	a versatile technique	121:141	a versatile technique for fabrication of made-on-purpose biomimetic scaffolds	121:197	Electrospinning is a versatile technique for fabrication of made-on-purpose biomimetic scaffolds.
32230742	1	50	theme	versatile	123:131	arg1	Electrospinning					102:116	Electrospinning	102:116	Electrospinning	102:116	Electrospinning is a versatile technique for fabrication of made-on-purpose biomimetic scaffolds.
32230742	10	51	theme	in	1363:1364	arg1	studies					1372:1378	in vitro studies	1363:1378	in vitro studies along with physicochemical characterizations	1363:1423	The results of in vitro studies along with physicochemical characterizations reflect the great potentials of the produced samples as scaffolds for application in skin tissue engineering.
32230742	2	52	theme	optimized	215:223	arg1	membranes					245:253	optimized electrospun fibrous membranes	215:253	optimized electrospun fibrous membranes	215:253	In this study, optimized electrospun fibrous membranes were produced by simultaneous electrospinning of polycaprolactone (PCL) and polyvinylpyrrolidone (PVP), followed by the selective removal of PVP from the PCL/PVP mesh.
32230742	6	53	theme	pvp	772:774	arg1	removal					761:767	removal	761:767	removal of pvp (from 4.46 μm before immersion in water to 33.55 μm after immersion in water for 24 h)	761:861	It seems that the positive trend of cell ingress originates from the proper pore size obtained after removal of pvp (from 4.46 μm before immersion in water to 33.55 μm after immersion in water for 24 h).
32230742	10	54	dep	in	1363:1364	arg1	vitro					1366:1370	vitro	1366:1370	vitro	1366:1370	The results of in vitro studies along with physicochemical characterizations reflect the great potentials of the produced samples as scaffolds for application in skin tissue engineering.
32230742	7	55	theme	keratinocyte	1015:1026	arg1	HaCaT					1040:1044	HaCaT	1040:1044	HaCaT	1040:1044	Furthermore, grafting the surface with the collagen/chitosan blend rendered the scaffolds more biocompatible with improved attachment and spreading of keratinocyte cell lines (HaCaT).
32230742	7	55	theme	keratinocyte	1015:1026	arg1	lines					1033:1037	keratinocyte cell lines	1015:1037	keratinocyte cell lines (HaCaT)	1015:1045	Furthermore, grafting the surface with the collagen/chitosan blend rendered the scaffolds more biocompatible with improved attachment and spreading of keratinocyte cell lines (HaCaT).
32230742	10	56	theme	physicochemical	1391:1405	arg1	characterizations					1407:1423	physicochemical characterizations	1391:1423	in vitro studies along with physicochemical characterizations	1363:1423	The results of in vitro studies along with physicochemical characterizations reflect the great potentials of the produced samples as scaffolds for application in skin tissue engineering.
32230742	6	57	theme	positive	678:685	arg1	trend					687:691	the positive trend	674:691	the positive trend of cell ingress	674:707	It seems that the positive trend of cell ingress originates from the proper pore size obtained after removal of pvp (from 4.46 μm before immersion in water to 33.55 μm after immersion in water for 24 h).
32230742	2	58	theme	simultaneous	272:283	arg1	electrospinning					285:299	simultaneous electrospinning	272:299	simultaneous electrospinning of polycaprolactone (PCL) and polyvinylpyrrolidone (PVP)	272:356	In this study, optimized electrospun fibrous membranes were produced by simultaneous electrospinning of polycaprolactone (PCL) and polyvinylpyrrolidone (PVP), followed by the selective removal of PVP from the PCL/PVP mesh.
32230742	2	59	from	mesh	417:420	arg1	removal					385:391	the selective removal	371:391	the selective removal of PVP from the PCL/PVP mesh	371:420	In this study, optimized electrospun fibrous membranes were produced by simultaneous electrospinning of polycaprolactone (PCL) and polyvinylpyrrolidone (PVP), followed by the selective removal of PVP from the PCL/PVP mesh.
32230742	9	60	theme	chitosan	1290:1297	arg1	effects					1279:1285	the bactericidal effects	1262:1285	the bactericidal effects of chitosan utilized in the composition of the coated blend	1262:1345	Antibacterial assay with Staphylococcus aureus as Gram-positive and Escherichia coli as Gram-negative species corroborated the bactericidal effects of chitosan utilized in the composition of the coated blend.
32230742	9	61	with	assay	1153:1157	arg1	coli					1219:1222	Escherichia coli	1207:1222	Escherichia coli	1207:1222	Antibacterial assay with Staphylococcus aureus as Gram-positive and Escherichia coli as Gram-negative species corroborated the bactericidal effects of chitosan utilized in the composition of the coated blend.
32230742	9	61	with	assay	1153:1157	arg1	aureus					1179:1184	Staphylococcus aureus	1164:1184	Staphylococcus aureus as Gram-positive	1164:1201	Antibacterial assay with Staphylococcus aureus as Gram-positive and Escherichia coli as Gram-negative species corroborated the bactericidal effects of chitosan utilized in the composition of the coated blend.
32230742	0	62	theme	PVP	61:63	arg1	Fibers					65:70	Sacrificial PVP Fibers	49:70	Sacrificial PVP Fibers	49:70	Tailored PCL Scaffolds as Skin Substitutes Using Sacrificial PVP Fibers and Collagen/Chitosan Blends.
32230742	7	63	theme	improved	978:985	arg1	attachment					987:996	improved attachment	978:996	improved attachment	978:996	Furthermore, grafting the surface with the collagen/chitosan blend rendered the scaffolds more biocompatible with improved attachment and spreading of keratinocyte cell lines (HaCaT).
32230742	10	64	from	application	1495:1505	arg1	engineering					1522:1532	skin tissue engineering	1510:1532	skin tissue engineering	1510:1532	The results of in vitro studies along with physicochemical characterizations reflect the great potentials of the produced samples as scaffolds for application in skin tissue engineering.
32173442	8	0	theme	inhibition	1277:1286	arg1	zones					1288:1292	high inhibition zones	1272:1292	high inhibition zones against Gram positive and Gram Negative bacteria (Escherichia coli, streptococcus mutans, Staphylococcus aureus and Pseudomonas aeruginosa)	1272:1432	Antibacterial analysis of the films shows high inhibition zones against Gram positive and Gram Negative bacteria (Escherichia coli, streptococcus mutans, Staphylococcus aureus and Pseudomonas aeruginosa).
32173442	9	1	theme	bone	1527:1530	arg1	application					1545:1555	bone regeneration application	1527:1555	bone regeneration application	1527:1555	Thus, the obtained nanocomposites bio-films are highly biocompatible and it can be used for bone regeneration application.
32173442	1	2	theme	surgical	181:188	arg1	sutures					190:196	surgical sutures	181:196	surgical sutures	181:196	Recently, polymer based biomaterials are utilized in medical fields including surgical sutures, drug delivery devices, tissue supports and implants for interior bone fixation.
32173442	8	3	dep	bacteria	1334:1341	arg1	Escherichia					1344:1354	Escherichia	1344:1354	Escherichia	1344:1354	Antibacterial analysis of the films shows high inhibition zones against Gram positive and Gram Negative bacteria (Escherichia coli, streptococcus mutans, Staphylococcus aureus and Pseudomonas aeruginosa).
32173442	8	3	dep	bacteria	1334:1341	arg1	streptococcus					1362:1374	streptococcus	1362:1374	streptococcus	1362:1374	Antibacterial analysis of the films shows high inhibition zones against Gram positive and Gram Negative bacteria (Escherichia coli, streptococcus mutans, Staphylococcus aureus and Pseudomonas aeruginosa).
32173442	8	3	dep	bacteria	1334:1341	arg1	Staphylococcus					1384:1397	Staphylococcus	1384:1397	Staphylococcus	1384:1397	Antibacterial analysis of the films shows high inhibition zones against Gram positive and Gram Negative bacteria (Escherichia coli, streptococcus mutans, Staphylococcus aureus and Pseudomonas aeruginosa).
32173442	8	3	dep	bacteria	1334:1341	arg1	Pseudomonas					1410:1420	Pseudomonas	1410:1420	Pseudomonas	1410:1420	Antibacterial analysis of the films shows high inhibition zones against Gram positive and Gram Negative bacteria (Escherichia coli, streptococcus mutans, Staphylococcus aureus and Pseudomonas aeruginosa).
32173442	4	4	theme	polymeric	686:694	arg1	film					696:699	polymeric film	686:699	polymeric film	686:699	Graphene oxide/Hydroxyapatite/gold nanocomposite (GO/HAP/Au) was synthesized by simple hydrothermal method and GO/HAP/Au nanocomposite incorporated polymeric film was fabricated using gel casting method.
32173442	8	5	theme	high	1272:1275	arg1	zones					1288:1292	high inhibition zones	1272:1292	high inhibition zones against Gram positive and Gram Negative bacteria (Escherichia coli, streptococcus mutans, Staphylococcus aureus and Pseudomonas aeruginosa)	1272:1432	Antibacterial analysis of the films shows high inhibition zones against Gram positive and Gram Negative bacteria (Escherichia coli, streptococcus mutans, Staphylococcus aureus and Pseudomonas aeruginosa).
32173442	5	6	theme	phase	758:762	arg1	composition					764:774	phase composition	758:774	phase composition	758:774	The morphology, phase composition, crystalline structure and chemical state of the nanocomposite were characterized using as XRD, HR-TEM, FE-SEM and FT-IR.
32173442	9	7	theme	obtained	1445:1452	arg1	biocompatible					1490:1502	biocompatible	1490:1502	biocompatible	1490:1502	Thus, the obtained nanocomposites bio-films are highly biocompatible and it can be used for bone regeneration application.
32173442	9	7	theme	obtained	1445:1452	arg1	bio-films					1469:1477	the obtained nanocomposites bio-films	1441:1477	the obtained nanocomposites bio-films	1441:1477	Thus, the obtained nanocomposites bio-films are highly biocompatible and it can be used for bone regeneration application.
32173442	4	8	theme	gel	722:724	arg1	method					734:739	gel casting method	722:739	gel casting method	722:739	Graphene oxide/Hydroxyapatite/gold nanocomposite (GO/HAP/Au) was synthesized by simple hydrothermal method and GO/HAP/Au nanocomposite incorporated polymeric film was fabricated using gel casting method.
32173442	4	9	theme	Graphene	538:545	arg1	GO/HAP/Au					588:596	GO/HAP/Au	588:596	GO/HAP/Au	588:596	Graphene oxide/Hydroxyapatite/gold nanocomposite (GO/HAP/Au) was synthesized by simple hydrothermal method and GO/HAP/Au nanocomposite incorporated polymeric film was fabricated using gel casting method.
32173442	4	9	theme	Graphene	538:545	arg1	nanocomposite					573:585	Graphene oxide/Hydroxyapatite/gold nanocomposite	538:585	Graphene oxide/Hydroxyapatite/gold nanocomposite (GO/HAP/Au)	538:597	Graphene oxide/Hydroxyapatite/gold nanocomposite (GO/HAP/Au) was synthesized by simple hydrothermal method and GO/HAP/Au nanocomposite incorporated polymeric film was fabricated using gel casting method.
32173442	6	10	theme	phosphatase	1045:1055	arg1	activity					1057:1064	more alkaline phosphatase activity	1031:1064	more alkaline phosphatase activity at the cellular level	1031:1086	The bio-films were found to be biocompatible with mouse mesenchymal cells and it enhanced osteoblast differentiation as evidenced by more alkaline phosphatase activity at the cellular level.
32173442	8	11	dep	Pseudomonas	1410:1420	arg1	aeruginosa					1422:1431	aeruginosa	1422:1431	aeruginosa	1422:1431	Antibacterial analysis of the films shows high inhibition zones against Gram positive and Gram Negative bacteria (Escherichia coli, streptococcus mutans, Staphylococcus aureus and Pseudomonas aeruginosa).
32173442	1	12	theme	medical	156:162	arg1	tissue					222:227	tissue supports	222:236	tissue supports	222:236	Recently, polymer based biomaterials are utilized in medical fields including surgical sutures, drug delivery devices, tissue supports and implants for interior bone fixation.
32173442	1	12	theme	medical	156:162	arg1	implants					242:249	implants	242:249	implants for interior bone fixation	242:276	Recently, polymer based biomaterials are utilized in medical fields including surgical sutures, drug delivery devices, tissue supports and implants for interior bone fixation.
32173442	1	12	theme	medical	156:162	arg1	devices					213:219	drug delivery devices	199:219	drug delivery devices	199:219	Recently, polymer based biomaterials are utilized in medical fields including surgical sutures, drug delivery devices, tissue supports and implants for interior bone fixation.
32173442	1	12	theme	medical	156:162	arg1	fields					164:169	medical fields	156:169	medical fields including surgical sutures, drug delivery devices, tissue supports and implants for interior bone fixation	156:276	Recently, polymer based biomaterials are utilized in medical fields including surgical sutures, drug delivery devices, tissue supports and implants for interior bone fixation.
32173442	1	12	theme	medical	156:162	arg1	sutures					190:196	surgical sutures	181:196	surgical sutures	181:196	Recently, polymer based biomaterials are utilized in medical fields including surgical sutures, drug delivery devices, tissue supports and implants for interior bone fixation.
32173442	8	13	theme	positive	1307:1314	arg1	bacteria					1334:1341	Gram positive and Gram Negative bacteria	1302:1341	Gram positive and Gram Negative bacteria (Escherichia coli, streptococcus mutans, Staphylococcus aureus and Pseudomonas aeruginosa)	1302:1432	Antibacterial analysis of the films shows high inhibition zones against Gram positive and Gram Negative bacteria (Escherichia coli, streptococcus mutans, Staphylococcus aureus and Pseudomonas aeruginosa).
32173442	6	14	theme	osteoblast	988:997	arg1	differentiation					999:1013	osteoblast differentiation	988:1013	osteoblast differentiation	988:1013	The bio-films were found to be biocompatible with mouse mesenchymal cells and it enhanced osteoblast differentiation as evidenced by more alkaline phosphatase activity at the cellular level.
32173442	8	15	theme	films	1260:1264	arg1	analysis					1244:1251	Antibacterial analysis	1230:1251	Antibacterial analysis of the films	1230:1264	Antibacterial analysis of the films shows high inhibition zones against Gram positive and Gram Negative bacteria (Escherichia coli, streptococcus mutans, Staphylococcus aureus and Pseudomonas aeruginosa).
32173442	2	16	theme	based	296:300	arg1	implants					302:309	polymer based implants	288:309	polymer based implants	288:309	However, polymer based implants leads to the formation of bio-films that are highly susceptible to microbial adhesion.
32173442	1	17	theme	delivery	204:211	arg1	devices					213:219	drug delivery devices	199:219	drug delivery devices	199:219	Recently, polymer based biomaterials are utilized in medical fields including surgical sutures, drug delivery devices, tissue supports and implants for interior bone fixation.
32173442	3	18	theme	oxide/Hydroxyapatite/gold	468:492	arg1	films					494:498	Chitosan/Polyvinyl alcohol/Graphene oxide/Hydroxyapatite/gold films	432:498	Chitosan/Polyvinyl alcohol/Graphene oxide/Hydroxyapatite/gold films	432:498	In this study, we have fabricated Chitosan/Polyvinyl alcohol/Graphene oxide/Hydroxyapatite/gold films for potential orthopedic application.
32173442	9	19	theme	nanocomposites	1454:1467	arg1	biocompatible					1490:1502	biocompatible	1490:1502	biocompatible	1490:1502	Thus, the obtained nanocomposites bio-films are highly biocompatible and it can be used for bone regeneration application.
32173442	9	19	theme	nanocomposites	1454:1467	arg1	bio-films					1469:1477	the obtained nanocomposites bio-films	1441:1477	the obtained nanocomposites bio-films	1441:1477	Thus, the obtained nanocomposites bio-films are highly biocompatible and it can be used for bone regeneration application.
32173442	2	20	theme	polymer	288:294	arg1	implants					302:309	polymer based implants	288:309	polymer based implants	288:309	However, polymer based implants leads to the formation of bio-films that are highly susceptible to microbial adhesion.
32173442	1	21	theme	drug	199:202	arg1	devices					213:219	drug delivery devices	199:219	drug delivery devices	199:219	Recently, polymer based biomaterials are utilized in medical fields including surgical sutures, drug delivery devices, tissue supports and implants for interior bone fixation.
32173442	9	22	used	used	1518:1521	arg2	it					1508:1509	it	1508:1509	it	1508:1509	Thus, the obtained nanocomposites bio-films are highly biocompatible and it can be used for bone regeneration application.
32173442	4	23	theme	oxide/Hydroxyapatite/gold	547:571	arg1	GO/HAP/Au					588:596	GO/HAP/Au	588:596	GO/HAP/Au	588:596	Graphene oxide/Hydroxyapatite/gold nanocomposite (GO/HAP/Au) was synthesized by simple hydrothermal method and GO/HAP/Au nanocomposite incorporated polymeric film was fabricated using gel casting method.
32173442	4	23	theme	oxide/Hydroxyapatite/gold	547:571	arg1	nanocomposite					573:585	Graphene oxide/Hydroxyapatite/gold nanocomposite	538:585	Graphene oxide/Hydroxyapatite/gold nanocomposite (GO/HAP/Au)	538:597	Graphene oxide/Hydroxyapatite/gold nanocomposite (GO/HAP/Au) was synthesized by simple hydrothermal method and GO/HAP/Au nanocomposite incorporated polymeric film was fabricated using gel casting method.
32173442	8	24	dep	Staphylococcus	1384:1397	arg1	aureus					1399:1404	aureus	1399:1404	aureus	1399:1404	Antibacterial analysis of the films shows high inhibition zones against Gram positive and Gram Negative bacteria (Escherichia coli, streptococcus mutans, Staphylococcus aureus and Pseudomonas aeruginosa).
32173442	8	25	theme	Antibacterial	1230:1242	arg1	analysis					1244:1251	Antibacterial analysis	1230:1251	Antibacterial analysis of the films	1230:1264	Antibacterial analysis of the films shows high inhibition zones against Gram positive and Gram Negative bacteria (Escherichia coli, streptococcus mutans, Staphylococcus aureus and Pseudomonas aeruginosa).
32173442	3	26	theme	Chitosan/Polyvinyl	432:449	arg1	films					494:498	Chitosan/Polyvinyl alcohol/Graphene oxide/Hydroxyapatite/gold films	432:498	Chitosan/Polyvinyl alcohol/Graphene oxide/Hydroxyapatite/gold films	432:498	In this study, we have fabricated Chitosan/Polyvinyl alcohol/Graphene oxide/Hydroxyapatite/gold films for potential orthopedic application.
32173442	0	27	theme	Nanocomposite	0:12	arg1	chitosan					14:21	Nanocomposite chitosan film	0:26	Nanocomposite chitosan film	0:26	Nanocomposite chitosan film containing graphene oxide/hydroxyapatite/gold for bone tissue engineering.
32173442	6	28	theme	alkaline	1036:1043	arg1	phosphatase					1045:1055	more alkaline phosphatase	1031:1055	more alkaline phosphatase activity at the cellular level	1031:1086	The bio-films were found to be biocompatible with mouse mesenchymal cells and it enhanced osteoblast differentiation as evidenced by more alkaline phosphatase activity at the cellular level.
32173442	1	29	theme	polymer	113:119	arg1	biomaterials					127:138	polymer based biomaterials	113:138	polymer based biomaterials	113:138	Recently, polymer based biomaterials are utilized in medical fields including surgical sutures, drug delivery devices, tissue supports and implants for interior bone fixation.
32173442	3	30	theme	alcohol/Graphene	451:466	arg1	films					494:498	Chitosan/Polyvinyl alcohol/Graphene oxide/Hydroxyapatite/gold films	432:498	Chitosan/Polyvinyl alcohol/Graphene oxide/Hydroxyapatite/gold films	432:498	In this study, we have fabricated Chitosan/Polyvinyl alcohol/Graphene oxide/Hydroxyapatite/gold films for potential orthopedic application.
32173442	1	31	theme	based	121:125	arg1	biomaterials					127:138	polymer based biomaterials	113:138	polymer based biomaterials	113:138	Recently, polymer based biomaterials are utilized in medical fields including surgical sutures, drug delivery devices, tissue supports and implants for interior bone fixation.
32173442	3	32	theme	orthopedic	514:523	arg1	application					525:535	potential orthopedic application	504:535	potential orthopedic application	504:535	In this study, we have fabricated Chitosan/Polyvinyl alcohol/Graphene oxide/Hydroxyapatite/gold films for potential orthopedic application.
32173442	8	33	theme	Negative	1325:1332	arg1	bacteria					1334:1341	Gram positive and Gram Negative bacteria	1302:1341	Gram positive and Gram Negative bacteria (Escherichia coli, streptococcus mutans, Staphylococcus aureus and Pseudomonas aeruginosa)	1302:1432	Antibacterial analysis of the films shows high inhibition zones against Gram positive and Gram Negative bacteria (Escherichia coli, streptococcus mutans, Staphylococcus aureus and Pseudomonas aeruginosa).
32173442	6	34	theme	cellular	1073:1080	arg1	level					1082:1086	the cellular level	1069:1086	the cellular level	1069:1086	The bio-films were found to be biocompatible with mouse mesenchymal cells and it enhanced osteoblast differentiation as evidenced by more alkaline phosphatase activity at the cellular level.
32173442	7	35	theme	bone	1198:1201	arg1	diseases					1220:1227	bone and bone-related diseases	1198:1227	diseases	1220:1227	Hence, these results suggested that the developed nanocomposites films are osteogenic potential for treating bone and bone-related diseases.
32173442	8	36	dep	streptococcus	1362:1374	arg1	mutans					1376:1381	mutans	1376:1381	mutans	1376:1381	Antibacterial analysis of the films shows high inhibition zones against Gram positive and Gram Negative bacteria (Escherichia coli, streptococcus mutans, Staphylococcus aureus and Pseudomonas aeruginosa).
32173442	4	37	theme	GO/HAP/Au	649:657	arg1	nanocomposite					659:671	GO/HAP/Au nanocomposite	649:671	GO/HAP/Au nanocomposite incorporated polymeric film	649:699	Graphene oxide/Hydroxyapatite/gold nanocomposite (GO/HAP/Au) was synthesized by simple hydrothermal method and GO/HAP/Au nanocomposite incorporated polymeric film was fabricated using gel casting method.
32173442	8	38	dep	Escherichia	1344:1354	arg1	coli					1356:1359	coli	1356:1359	coli	1356:1359	Antibacterial analysis of the films shows high inhibition zones against Gram positive and Gram Negative bacteria (Escherichia coli, streptococcus mutans, Staphylococcus aureus and Pseudomonas aeruginosa).
32173442	0	39	theme	graphene	39:46	arg1	oxide/hydroxyapatite/gold					48:72	graphene oxide/hydroxyapatite/gold	39:72	graphene oxide/hydroxyapatite/gold for bone tissue engineering	39:100	Nanocomposite chitosan film containing graphene oxide/hydroxyapatite/gold for bone tissue engineering.
32173442	5	40	theme	nanocomposite	825:837	arg1	morphology					746:755	The morphology	742:755	The morphology	742:755	The morphology, phase composition, crystalline structure and chemical state of the nanocomposite were characterized using as XRD, HR-TEM, FE-SEM and FT-IR.
32173442	5	40	theme	nanocomposite	825:837	arg1	composition					764:774	phase composition	758:774	phase composition	758:774	The morphology, phase composition, crystalline structure and chemical state of the nanocomposite were characterized using as XRD, HR-TEM, FE-SEM and FT-IR.
32173442	5	40	theme	nanocomposite	825:837	arg1	structure					789:797	crystalline structure	777:797	crystalline structure	777:797	The morphology, phase composition, crystalline structure and chemical state of the nanocomposite were characterized using as XRD, HR-TEM, FE-SEM and FT-IR.
32173442	5	40	theme	nanocomposite	825:837	arg1	state					812:816	chemical state	803:816	chemical state	803:816	The morphology, phase composition, crystalline structure and chemical state of the nanocomposite were characterized using as XRD, HR-TEM, FE-SEM and FT-IR.
32173442	6	41	theme	mesenchymal	954:964	arg1	cells					966:970	mouse mesenchymal cells	948:970	mouse mesenchymal cells	948:970	The bio-films were found to be biocompatible with mouse mesenchymal cells and it enhanced osteoblast differentiation as evidenced by more alkaline phosphatase activity at the cellular level.
32173442	4	42	theme	casting	726:732	arg1	method					734:739	gel casting method	722:739	gel casting method	722:739	Graphene oxide/Hydroxyapatite/gold nanocomposite (GO/HAP/Au) was synthesized by simple hydrothermal method and GO/HAP/Au nanocomposite incorporated polymeric film was fabricated using gel casting method.
32173442	3	43	theme	potential	504:512	arg1	application					525:535	potential orthopedic application	504:535	potential orthopedic application	504:535	In this study, we have fabricated Chitosan/Polyvinyl alcohol/Graphene oxide/Hydroxyapatite/gold films for potential orthopedic application.
32173442	0	44	theme	bone	78:81	arg1	engineering					90:100	bone tissue engineering	78:100	bone tissue engineering	78:100	Nanocomposite chitosan film containing graphene oxide/hydroxyapatite/gold for bone tissue engineering.
32173442	5	45	theme	crystalline	777:787	arg1	structure					789:797	crystalline structure	777:797	crystalline structure	777:797	The morphology, phase composition, crystalline structure and chemical state of the nanocomposite were characterized using as XRD, HR-TEM, FE-SEM and FT-IR.
32173442	6	46	theme	mouse	948:952	arg1	cells					966:970	mouse mesenchymal cells	948:970	mouse mesenchymal cells	948:970	The bio-films were found to be biocompatible with mouse mesenchymal cells and it enhanced osteoblast differentiation as evidenced by more alkaline phosphatase activity at the cellular level.
32173442	7	47	theme	developed	1129:1137	arg1	films					1154:1158	the developed nanocomposites films	1125:1158	the developed nanocomposites films	1125:1158	Hence, these results suggested that the developed nanocomposites films are osteogenic potential for treating bone and bone-related diseases.
32173442	7	47	theme	developed	1129:1137	arg1	potential					1175:1183	osteogenic potential	1164:1183	osteogenic potential for treating bone and bone-related diseases	1164:1227	Hence, these results suggested that the developed nanocomposites films are osteogenic potential for treating bone and bone-related diseases.
32173442	6	48	from	level	1082:1086	arg1	activity					1057:1064	more alkaline phosphatase activity	1031:1064	more alkaline phosphatase activity at the cellular level	1031:1086	The bio-films were found to be biocompatible with mouse mesenchymal cells and it enhanced osteoblast differentiation as evidenced by more alkaline phosphatase activity at the cellular level.
32173442	4	49	theme	hydrothermal	625:636	arg1	method					638:643	simple hydrothermal method	618:643	simple hydrothermal method	618:643	Graphene oxide/Hydroxyapatite/gold nanocomposite (GO/HAP/Au) was synthesized by simple hydrothermal method and GO/HAP/Au nanocomposite incorporated polymeric film was fabricated using gel casting method.
32173442	7	50	theme	osteogenic	1164:1173	arg1	films					1154:1158	the developed nanocomposites films	1125:1158	the developed nanocomposites films	1125:1158	Hence, these results suggested that the developed nanocomposites films are osteogenic potential for treating bone and bone-related diseases.
32173442	7	50	theme	osteogenic	1164:1173	arg1	potential					1175:1183	osteogenic potential	1164:1183	osteogenic potential for treating bone and bone-related diseases	1164:1227	Hence, these results suggested that the developed nanocomposites films are osteogenic potential for treating bone and bone-related diseases.
32173442	4	51	theme	simple	618:623	arg1	method					638:643	simple hydrothermal method	618:643	simple hydrothermal method	618:643	Graphene oxide/Hydroxyapatite/gold nanocomposite (GO/HAP/Au) was synthesized by simple hydrothermal method and GO/HAP/Au nanocomposite incorporated polymeric film was fabricated using gel casting method.
32173442	7	52	theme	nanocomposites	1139:1152	arg1	films					1154:1158	the developed nanocomposites films	1125:1158	the developed nanocomposites films	1125:1158	Hence, these results suggested that the developed nanocomposites films are osteogenic potential for treating bone and bone-related diseases.
32173442	7	52	theme	nanocomposites	1139:1152	arg1	potential					1175:1183	osteogenic potential	1164:1183	osteogenic potential for treating bone and bone-related diseases	1164:1227	Hence, these results suggested that the developed nanocomposites films are osteogenic potential for treating bone and bone-related diseases.
32173442	1	53	used	utilized	144:151	arg2	biomaterials					127:138	polymer based biomaterials	113:138	polymer based biomaterials	113:138	Recently, polymer based biomaterials are utilized in medical fields including surgical sutures, drug delivery devices, tissue supports and implants for interior bone fixation.
32173442	5	54	theme	chemical	803:810	arg1	state					812:816	chemical state	803:816	chemical state	803:816	The morphology, phase composition, crystalline structure and chemical state of the nanocomposite were characterized using as XRD, HR-TEM, FE-SEM and FT-IR.
32173442	9	55	theme	regeneration	1532:1543	arg1	application					1545:1555	bone regeneration application	1527:1555	bone regeneration application	1527:1555	Thus, the obtained nanocomposites bio-films are highly biocompatible and it can be used for bone regeneration application.
32173442	2	56	theme	bio-films	337:345	arg1	formation					324:332	the formation	320:332	the formation of bio-films that are highly susceptible to microbial adhesion	320:395	However, polymer based implants leads to the formation of bio-films that are highly susceptible to microbial adhesion.
32173442	7	57	theme	bone-related	1207:1218	arg1	diseases					1220:1227	bone and bone-related diseases	1198:1227	diseases	1220:1227	Hence, these results suggested that the developed nanocomposites films are osteogenic potential for treating bone and bone-related diseases.
32173442	6	58	with	biocompatible	929:941	arg1	cells					966:970	mouse mesenchymal cells	948:970	mouse mesenchymal cells	948:970	The bio-films were found to be biocompatible with mouse mesenchymal cells and it enhanced osteoblast differentiation as evidenced by more alkaline phosphatase activity at the cellular level.
32173442	1	59	theme	interior	255:262	arg1	fixation					269:276	interior bone fixation	255:276	interior bone fixation	255:276	Recently, polymer based biomaterials are utilized in medical fields including surgical sutures, drug delivery devices, tissue supports and implants for interior bone fixation.
32173442	0	60	theme	tissue	83:88	arg1	engineering					90:100	bone tissue engineering	78:100	bone tissue engineering	78:100	Nanocomposite chitosan film containing graphene oxide/hydroxyapatite/gold for bone tissue engineering.
32173442	1	61	dep	tissue	222:227	arg1	supports					229:236	supports	229:236	supports	229:236	Recently, polymer based biomaterials are utilized in medical fields including surgical sutures, drug delivery devices, tissue supports and implants for interior bone fixation.
32173442	2	62	theme	microbial	378:386	arg1	adhesion					388:395	microbial adhesion	378:395	microbial adhesion	378:395	However, polymer based implants leads to the formation of bio-films that are highly susceptible to microbial adhesion.
32173442	1	63	theme	bone	264:267	arg1	fixation					269:276	interior bone fixation	255:276	interior bone fixation	255:276	Recently, polymer based biomaterials are utilized in medical fields including surgical sutures, drug delivery devices, tissue supports and implants for interior bone fixation.
32950139	0	0	theme	mold	70:73	arg1	growth					75:80	mold growth	70:80	mold growth on PDO Pecorino Toscano cheese rind	70:116	Use of chitosan and tannins as alternatives to antibiotics to control mold growth on PDO Pecorino Toscano cheese rind.
32950139	6	1	theme	Toscano	1433:1439	arg1	ripening					1448:1455	PDO Pecorino Toscano cheese ripening	1420:1455	PDO Pecorino Toscano cheese ripening	1420:1455	Overall, grape marc tannins and chitosan did not significantly affect the number and composition of fungal communities developing during PDO Pecorino Toscano cheese ripening, as well as its physical, chemical and nutritional profiles, showing that they may represent effective alternatives to the antibiotic natamycin.
32950139	4	2	theme	cheese	892:897	arg1	communities					857:867	rind fungal communities	845:867	rind fungal communities of PDO Tuscan pecorino cheese	845:897	Morphological and molecular methods revealed that the main components of rind fungal communities of PDO Tuscan pecorino cheese were represented by P. solitum, P. discolour and P. verrucosum.
32950139	3	3	dep	tannins	675:681	arg1	i.e.					670:673	i.e.	670:673	i.e.	670:673	Here, agroindustrial by-products with natural antimicrobial properties, i.e. tannins and chitosan, were tested in a cheese-making trial producing PDO Tuscan pecorino cheese.
32950139	6	4	theme	Pecorino	1424:1431	arg1	ripening					1448:1455	PDO Pecorino Toscano cheese ripening	1420:1455	PDO Pecorino Toscano cheese ripening	1420:1455	Overall, grape marc tannins and chitosan did not significantly affect the number and composition of fungal communities developing during PDO Pecorino Toscano cheese ripening, as well as its physical, chemical and nutritional profiles, showing that they may represent effective alternatives to the antibiotic natamycin.
32950139	5	5	theme	whole	1093:1097	arg1	process					1108:1114	the whole ripening process	1089:1114	the whole ripening process compared with controls treated with natamycin, whose numbers ranged from 3.4 ± 1.3 × 103 to 3.2 ± 1.8 × 104 and from 6.3 ± 3.5 × 102 to 4.0 ± 1.5 × 104, respectively	1089:1280	The use of chitosan on cheese rinds did not significantly affect the composition of rind fungal communities developing during the whole ripening process compared with controls treated with natamycin, whose numbers ranged from 3.4 ± 1.3 × 103 to 3.2 ± 1.8 × 104 and from 6.3 ± 3.5 × 102 to 4.0 ± 1.5 × 104, respectively.
32950139	4	6	theme	pecorino	883:890	arg1	cheese					892:897	PDO Tuscan pecorino cheese	872:897	PDO Tuscan pecorino cheese	872:897	Morphological and molecular methods revealed that the main components of rind fungal communities of PDO Tuscan pecorino cheese were represented by P. solitum, P. discolour and P. verrucosum.
32950139	6	7	theme	marc	1298:1301	arg1	tannins					1303:1309	grape marc tannins	1292:1309	grape marc tannins	1292:1309	Overall, grape marc tannins and chitosan did not significantly affect the number and composition of fungal communities developing during PDO Pecorino Toscano cheese ripening, as well as its physical, chemical and nutritional profiles, showing that they may represent effective alternatives to the antibiotic natamycin.
32950139	5	8	theme	ripening	1099:1106	arg1	process					1108:1114	the whole ripening process	1089:1114	the whole ripening process compared with controls treated with natamycin, whose numbers ranged from 3.4 ± 1.3 × 103 to 3.2 ± 1.8 × 104 and from 6.3 ± 3.5 × 102 to 4.0 ± 1.5 × 104, respectively	1089:1280	The use of chitosan on cheese rinds did not significantly affect the composition of rind fungal communities developing during the whole ripening process compared with controls treated with natamycin, whose numbers ranged from 3.4 ± 1.3 × 103 to 3.2 ± 1.8 × 104 and from 6.3 ± 3.5 × 102 to 4.0 ± 1.5 × 104, respectively.
32950139	6	9	theme	nutritional	1496:1506	arg1	profiles					1508:1515	its physical, chemical and nutritional profiles	1469:1515	its physical, chemical and nutritional profiles	1469:1515	Overall, grape marc tannins and chitosan did not significantly affect the number and composition of fungal communities developing during PDO Pecorino Toscano cheese ripening, as well as its physical, chemical and nutritional profiles, showing that they may represent effective alternatives to the antibiotic natamycin.
32950139	5	10	theme	cheese	986:991	arg1	rinds					993:997	cheese rinds	986:997	cheese rinds	986:997	The use of chitosan on cheese rinds did not significantly affect the composition of rind fungal communities developing during the whole ripening process compared with controls treated with natamycin, whose numbers ranged from 3.4 ± 1.3 × 103 to 3.2 ± 1.8 × 104 and from 6.3 ± 3.5 × 102 to 4.0 ± 1.5 × 104, respectively.
32950139	6	11	theme	PDO	1420:1422	arg1	ripening					1448:1455	PDO Pecorino Toscano cheese ripening	1420:1455	PDO Pecorino Toscano cheese ripening	1420:1455	Overall, grape marc tannins and chitosan did not significantly affect the number and composition of fungal communities developing during PDO Pecorino Toscano cheese ripening, as well as its physical, chemical and nutritional profiles, showing that they may represent effective alternatives to the antibiotic natamycin.
32950139	5	12	theme	chitosan	974:981	arg1	use					967:969	The use	963:969	The use of chitosan on cheese rinds	963:997	The use of chitosan on cheese rinds did not significantly affect the composition of rind fungal communities developing during the whole ripening process compared with controls treated with natamycin, whose numbers ranged from 3.4 ± 1.3 × 103 to 3.2 ± 1.8 × 104 and from 6.3 ± 3.5 × 102 to 4.0 ± 1.5 × 104, respectively.
32950139	4	13	theme	Morphological	772:784	arg1	methods					800:806	Morphological and molecular methods	772:806	Morphological and molecular methods	772:806	Morphological and molecular methods revealed that the main components of rind fungal communities of PDO Tuscan pecorino cheese were represented by P. solitum, P. discolour and P. verrucosum.
32950139	1	14	theme	organoleptic	251:262	arg1	characteristics					264:278	organoleptic characteristics	251:278	organoleptic characteristics	251:278	The fungal microbiota usually growing on the cheese surface during ripening processes promote rind formation and the development of organoleptic characteristics, imparting positive sensory attributes to cheeses.
32950139	0	15	theme	Pecorino	89:96	arg1	rind					113:116	PDO Pecorino Toscano cheese rind	85:116	PDO Pecorino Toscano cheese rind	85:116	Use of chitosan and tannins as alternatives to antibiotics to control mold growth on PDO Pecorino Toscano cheese rind.
32950139	3	16	theme	PDO	744:746	arg1	cheese					764:769	PDO Tuscan pecorino cheese	744:769	PDO Tuscan pecorino cheese	744:769	Here, agroindustrial by-products with natural antimicrobial properties, i.e. tannins and chitosan, were tested in a cheese-making trial producing PDO Tuscan pecorino cheese.
32950139	2	17	theme	specific	392:399	arg1	actions					401:407	specific actions	392:407	specific actions for preventing their growth	392:435	As cheese contamination may also occur by undesirable molds, specific actions for preventing their growth are usually realized in dairy industries by using the antibiotic natamycin, which may represent a risk factor for human health and environmental sustainability.
32950139	0	18	theme	PDO	85:87	arg1	rind					113:116	PDO Pecorino Toscano cheese rind	85:116	PDO Pecorino Toscano cheese rind	85:116	Use of chitosan and tannins as alternatives to antibiotics to control mold growth on PDO Pecorino Toscano cheese rind.
32950139	3	19	theme	cheese-making	714:726	arg1	trial					728:732	a cheese-making trial	712:732	a cheese-making trial producing PDO Tuscan pecorino cheese	712:769	Here, agroindustrial by-products with natural antimicrobial properties, i.e. tannins and chitosan, were tested in a cheese-making trial producing PDO Tuscan pecorino cheese.
32950139	4	20	theme	communities	857:867	arg1	components					831:840	the main components	822:840	the main components of rind fungal communities of PDO Tuscan pecorino cheese	822:897	Morphological and molecular methods revealed that the main components of rind fungal communities of PDO Tuscan pecorino cheese were represented by P. solitum, P. discolour and P. verrucosum.
32950139	4	21	theme	molecular	790:798	arg1	methods					800:806	Morphological and molecular methods	772:806	Morphological and molecular methods	772:806	Morphological and molecular methods revealed that the main components of rind fungal communities of PDO Tuscan pecorino cheese were represented by P. solitum, P. discolour and P. verrucosum.
32950139	6	22	theme	antibiotic	1580:1589	arg1	natamycin					1591:1599	the antibiotic natamycin	1576:1599	the antibiotic natamycin	1576:1599	Overall, grape marc tannins and chitosan did not significantly affect the number and composition of fungal communities developing during PDO Pecorino Toscano cheese ripening, as well as its physical, chemical and nutritional profiles, showing that they may represent effective alternatives to the antibiotic natamycin.
32950139	6	23	theme	chemical	1483:1490	arg1	profiles					1508:1515	its physical, chemical and nutritional profiles	1469:1515	its physical, chemical and nutritional profiles	1469:1515	Overall, grape marc tannins and chitosan did not significantly affect the number and composition of fungal communities developing during PDO Pecorino Toscano cheese ripening, as well as its physical, chemical and nutritional profiles, showing that they may represent effective alternatives to the antibiotic natamycin.
32950139	3	24	theme	pecorino	755:762	arg1	cheese					764:769	PDO Tuscan pecorino cheese	744:769	PDO Tuscan pecorino cheese	744:769	Here, agroindustrial by-products with natural antimicrobial properties, i.e. tannins and chitosan, were tested in a cheese-making trial producing PDO Tuscan pecorino cheese.
32950139	4	25	theme	Tuscan	876:881	arg1	cheese					892:897	PDO Tuscan pecorino cheese	872:897	PDO Tuscan pecorino cheese	872:897	Morphological and molecular methods revealed that the main components of rind fungal communities of PDO Tuscan pecorino cheese were represented by P. solitum, P. discolour and P. verrucosum.
32950139	1	26	theme	characteristics	264:278	arg1	formation					218:226	rind formation	213:226	rind formation	213:226	The fungal microbiota usually growing on the cheese surface during ripening processes promote rind formation and the development of organoleptic characteristics, imparting positive sensory attributes to cheeses.
32950139	1	26	theme	characteristics	264:278	arg1	development					236:246	the development	232:246	the development of organoleptic characteristics	232:278	The fungal microbiota usually growing on the cheese surface during ripening processes promote rind formation and the development of organoleptic characteristics, imparting positive sensory attributes to cheeses.
32950139	4	27	theme	PDO	872:874	arg1	cheese					892:897	PDO Tuscan pecorino cheese	872:897	PDO Tuscan pecorino cheese	872:897	Morphological and molecular methods revealed that the main components of rind fungal communities of PDO Tuscan pecorino cheese were represented by P. solitum, P. discolour and P. verrucosum.
32950139	2	28	theme	undesirable	373:383	arg1	molds					385:389	undesirable molds	373:389	undesirable molds	373:389	As cheese contamination may also occur by undesirable molds, specific actions for preventing their growth are usually realized in dairy industries by using the antibiotic natamycin, which may represent a risk factor for human health and environmental sustainability.
32950139	6	29	theme	physical	1473:1480	arg1	profiles					1508:1515	its physical, chemical and nutritional profiles	1469:1515	its physical, chemical and nutritional profiles	1469:1515	Overall, grape marc tannins and chitosan did not significantly affect the number and composition of fungal communities developing during PDO Pecorino Toscano cheese ripening, as well as its physical, chemical and nutritional profiles, showing that they may represent effective alternatives to the antibiotic natamycin.
32950139	4	30	theme	main	826:829	arg1	components					831:840	the main components	822:840	the main components of rind fungal communities of PDO Tuscan pecorino cheese	822:897	Morphological and molecular methods revealed that the main components of rind fungal communities of PDO Tuscan pecorino cheese were represented by P. solitum, P. discolour and P. verrucosum.
32950139	0	31	theme	cheese	106:111	arg1	rind					113:116	PDO Pecorino Toscano cheese rind	85:116	PDO Pecorino Toscano cheese rind	85:116	Use of chitosan and tannins as alternatives to antibiotics to control mold growth on PDO Pecorino Toscano cheese rind.
32950139	6	32	theme	communities	1390:1400	arg1	profiles					1508:1515	its physical, chemical and nutritional profiles	1469:1515	its physical, chemical and nutritional profiles	1469:1515	Overall, grape marc tannins and chitosan did not significantly affect the number and composition of fungal communities developing during PDO Pecorino Toscano cheese ripening, as well as its physical, chemical and nutritional profiles, showing that they may represent effective alternatives to the antibiotic natamycin.
32950139	6	32	theme	communities	1390:1400	arg1	composition					1368:1378	composition	1368:1378	composition	1368:1378	Overall, grape marc tannins and chitosan did not significantly affect the number and composition of fungal communities developing during PDO Pecorino Toscano cheese ripening, as well as its physical, chemical and nutritional profiles, showing that they may represent effective alternatives to the antibiotic natamycin.
32950139	6	32	theme	communities	1390:1400	arg1	number					1357:1362	number	1357:1362	number	1357:1362	Overall, grape marc tannins and chitosan did not significantly affect the number and composition of fungal communities developing during PDO Pecorino Toscano cheese ripening, as well as its physical, chemical and nutritional profiles, showing that they may represent effective alternatives to the antibiotic natamycin.
32950139	0	33	theme	chitosan	7:14	arg1	Use					0:2	Use	0:2	Use of chitosan and tannins as alternatives to antibiotics	0:57	Use of chitosan and tannins as alternatives to antibiotics to control mold growth on PDO Pecorino Toscano cheese rind.
32950139	3	34	theme	Tuscan	748:753	arg1	cheese					764:769	PDO Tuscan pecorino cheese	744:769	PDO Tuscan pecorino cheese	744:769	Here, agroindustrial by-products with natural antimicrobial properties, i.e. tannins and chitosan, were tested in a cheese-making trial producing PDO Tuscan pecorino cheese.
32950139	0	35	theme	Toscano	98:104	arg1	rind					113:116	PDO Pecorino Toscano cheese rind	85:116	PDO Pecorino Toscano cheese rind	85:116	Use of chitosan and tannins as alternatives to antibiotics to control mold growth on PDO Pecorino Toscano cheese rind.
32950139	6	36	theme	effective	1550:1558	arg1	alternatives					1560:1571	effective alternatives	1550:1571	effective alternatives to the antibiotic natamycin	1550:1599	Overall, grape marc tannins and chitosan did not significantly affect the number and composition of fungal communities developing during PDO Pecorino Toscano cheese ripening, as well as its physical, chemical and nutritional profiles, showing that they may represent effective alternatives to the antibiotic natamycin.
32950139	1	37	theme	positive	291:298	arg1	attributes					308:317	positive sensory attributes	291:317	positive sensory attributes	291:317	The fungal microbiota usually growing on the cheese surface during ripening processes promote rind formation and the development of organoleptic characteristics, imparting positive sensory attributes to cheeses.
32950139	2	38	theme	risk	535:538	arg1	factor					540:545	a risk factor	533:545	a risk factor for human health and environmental sustainability	533:595	As cheese contamination may also occur by undesirable molds, specific actions for preventing their growth are usually realized in dairy industries by using the antibiotic natamycin, which may represent a risk factor for human health and environmental sustainability.
32950139	1	39	theme	cheese	164:169	arg1	surface					171:177	the cheese surface	160:177	the cheese surface	160:177	The fungal microbiota usually growing on the cheese surface during ripening processes promote rind formation and the development of organoleptic characteristics, imparting positive sensory attributes to cheeses.
32950139	1	40	theme	ripening	186:193	arg1	processes					195:203	ripening processes	186:203	ripening processes	186:203	The fungal microbiota usually growing on the cheese surface during ripening processes promote rind formation and the development of organoleptic characteristics, imparting positive sensory attributes to cheeses.
32950139	0	41	theme	tannins	20:26	arg1	Use					0:2	Use	0:2	Use of chitosan and tannins as alternatives to antibiotics	0:57	Use of chitosan and tannins as alternatives to antibiotics to control mold growth on PDO Pecorino Toscano cheese rind.
32950139	1	42	theme	sensory	300:306	arg1	attributes					308:317	positive sensory attributes	291:317	positive sensory attributes	291:317	The fungal microbiota usually growing on the cheese surface during ripening processes promote rind formation and the development of organoleptic characteristics, imparting positive sensory attributes to cheeses.
32950139	3	43	theme	antimicrobial	644:656	arg1	properties					658:667	natural antimicrobial properties	636:667	natural antimicrobial properties	636:667	Here, agroindustrial by-products with natural antimicrobial properties, i.e. tannins and chitosan, were tested in a cheese-making trial producing PDO Tuscan pecorino cheese.
32950139	6	44	dep	number	1357:1362	arg1	the					1353:1355	the	1353:1355	the	1353:1355	Overall, grape marc tannins and chitosan did not significantly affect the number and composition of fungal communities developing during PDO Pecorino Toscano cheese ripening, as well as its physical, chemical and nutritional profiles, showing that they may represent effective alternatives to the antibiotic natamycin.
32950139	6	45	theme	cheese	1441:1446	arg1	ripening					1448:1455	PDO Pecorino Toscano cheese ripening	1420:1455	PDO Pecorino Toscano cheese ripening	1420:1455	Overall, grape marc tannins and chitosan did not significantly affect the number and composition of fungal communities developing during PDO Pecorino Toscano cheese ripening, as well as its physical, chemical and nutritional profiles, showing that they may represent effective alternatives to the antibiotic natamycin.
32950139	2	46	theme	dairy	461:465	arg1	industries					467:476	dairy industries	461:476	dairy industries	461:476	As cheese contamination may also occur by undesirable molds, specific actions for preventing their growth are usually realized in dairy industries by using the antibiotic natamycin, which may represent a risk factor for human health and environmental sustainability.
32950139	2	47	theme	environmental	568:580	arg1	sustainability					582:595	environmental sustainability	568:595	environmental sustainability	568:595	As cheese contamination may also occur by undesirable molds, specific actions for preventing their growth are usually realized in dairy industries by using the antibiotic natamycin, which may represent a risk factor for human health and environmental sustainability.
32950139	3	48	with	by-products	619:629	arg1	properties					658:667	natural antimicrobial properties	636:667	natural antimicrobial properties	636:667	Here, agroindustrial by-products with natural antimicrobial properties, i.e. tannins and chitosan, were tested in a cheese-making trial producing PDO Tuscan pecorino cheese.
32950139	4	49	theme	fungal	850:855	arg1	communities					857:867	rind fungal communities	845:867	rind fungal communities of PDO Tuscan pecorino cheese	845:897	Morphological and molecular methods revealed that the main components of rind fungal communities of PDO Tuscan pecorino cheese were represented by P. solitum, P. discolour and P. verrucosum.
32950139	6	50	theme	fungal	1383:1388	arg1	communities					1390:1400	fungal communities	1383:1400	fungal communities developing during PDO Pecorino Toscano cheese ripening	1383:1455	Overall, grape marc tannins and chitosan did not significantly affect the number and composition of fungal communities developing during PDO Pecorino Toscano cheese ripening, as well as its physical, chemical and nutritional profiles, showing that they may represent effective alternatives to the antibiotic natamycin.
32950139	5	51	theme	rind	1047:1050	arg1	communities					1059:1069	rind fungal communities	1047:1069	rind fungal communities developing during the whole ripening process compared with controls treated with natamycin, whose numbers ranged from 3.4 ± 1.3 × 103 to 3.2 ± 1.8 × 104 and from 6.3 ± 3.5 × 102 to 4.0 ± 1.5 × 104, respectively	1047:1280	The use of chitosan on cheese rinds did not significantly affect the composition of rind fungal communities developing during the whole ripening process compared with controls treated with natamycin, whose numbers ranged from 3.4 ± 1.3 × 103 to 3.2 ± 1.8 × 104 and from 6.3 ± 3.5 × 102 to 4.0 ± 1.5 × 104, respectively.
32950139	4	52	theme	rind	845:848	arg1	communities					857:867	rind fungal communities	845:867	rind fungal communities of PDO Tuscan pecorino cheese	845:897	Morphological and molecular methods revealed that the main components of rind fungal communities of PDO Tuscan pecorino cheese were represented by P. solitum, P. discolour and P. verrucosum.
32950139	2	53	theme	cheese	334:339	arg1	contamination					341:353	cheese contamination	334:353	cheese contamination	334:353	As cheese contamination may also occur by undesirable molds, specific actions for preventing their growth are usually realized in dairy industries by using the antibiotic natamycin, which may represent a risk factor for human health and environmental sustainability.
32950139	5	54	theme	fungal	1052:1057	arg1	communities					1059:1069	rind fungal communities	1047:1069	rind fungal communities developing during the whole ripening process compared with controls treated with natamycin, whose numbers ranged from 3.4 ± 1.3 × 103 to 3.2 ± 1.8 × 104 and from 6.3 ± 3.5 × 102 to 4.0 ± 1.5 × 104, respectively	1047:1280	The use of chitosan on cheese rinds did not significantly affect the composition of rind fungal communities developing during the whole ripening process compared with controls treated with natamycin, whose numbers ranged from 3.4 ± 1.3 × 103 to 3.2 ± 1.8 × 104 and from 6.3 ± 3.5 × 102 to 4.0 ± 1.5 × 104, respectively.
32950139	6	55	theme	grape	1292:1296	arg1	tannins					1303:1309	grape marc tannins	1292:1309	grape marc tannins	1292:1309	Overall, grape marc tannins and chitosan did not significantly affect the number and composition of fungal communities developing during PDO Pecorino Toscano cheese ripening, as well as its physical, chemical and nutritional profiles, showing that they may represent effective alternatives to the antibiotic natamycin.
32950139	5	56	from	use	967:969	arg1	rinds					993:997	cheese rinds	986:997	cheese rinds	986:997	The use of chitosan on cheese rinds did not significantly affect the composition of rind fungal communities developing during the whole ripening process compared with controls treated with natamycin, whose numbers ranged from 3.4 ± 1.3 × 103 to 3.2 ± 1.8 × 104 and from 6.3 ± 3.5 × 102 to 4.0 ± 1.5 × 104, respectively.
32950139	2	57	theme	human	551:555	arg1	health					557:562	human health	551:562	human health	551:562	As cheese contamination may also occur by undesirable molds, specific actions for preventing their growth are usually realized in dairy industries by using the antibiotic natamycin, which may represent a risk factor for human health and environmental sustainability.
32950139	5	58	theme	communities	1059:1069	arg1	composition					1032:1042	the composition	1028:1042	the composition of rind fungal communities developing during the whole ripening process compared with controls treated with natamycin, whose numbers ranged from 3.4 ± 1.3 × 103 to 3.2 ± 1.8 × 104 and from 6.3 ± 3.5 × 102 to 4.0 ± 1.5 × 104, respectively	1028:1280	The use of chitosan on cheese rinds did not significantly affect the composition of rind fungal communities developing during the whole ripening process compared with controls treated with natamycin, whose numbers ranged from 3.4 ± 1.3 × 103 to 3.2 ± 1.8 × 104 and from 6.3 ± 3.5 × 102 to 4.0 ± 1.5 × 104, respectively.
32950139	3	59	theme	natural	636:642	arg1	properties					658:667	natural antimicrobial properties	636:667	natural antimicrobial properties	636:667	Here, agroindustrial by-products with natural antimicrobial properties, i.e. tannins and chitosan, were tested in a cheese-making trial producing PDO Tuscan pecorino cheese.
32950139	5	60	dep	4.0 ± 1.5 × 104	1252:1266	arg1	to					1249:1250	to	1249:1250	to	1249:1250	The use of chitosan on cheese rinds did not significantly affect the composition of rind fungal communities developing during the whole ripening process compared with controls treated with natamycin, whose numbers ranged from 3.4 ± 1.3 × 103 to 3.2 ± 1.8 × 104 and from 6.3 ± 3.5 × 102 to 4.0 ± 1.5 × 104, respectively.
32950139	1	61	theme	rind	213:216	arg1	formation					218:226	rind formation	213:226	rind formation	213:226	The fungal microbiota usually growing on the cheese surface during ripening processes promote rind formation and the development of organoleptic characteristics, imparting positive sensory attributes to cheeses.
32950139	0	62	from	growth	75:80	arg1	rind					113:116	PDO Pecorino Toscano cheese rind	85:116	PDO Pecorino Toscano cheese rind	85:116	Use of chitosan and tannins as alternatives to antibiotics to control mold growth on PDO Pecorino Toscano cheese rind.
32950139	3	63	theme	agroindustrial	604:617	arg1	by-products					619:629	agroindustrial by-products	604:629	agroindustrial by-products	604:629	Here, agroindustrial by-products with natural antimicrobial properties, i.e. tannins and chitosan, were tested in a cheese-making trial producing PDO Tuscan pecorino cheese.
32950139	2	64	theme	antibiotic	491:500	arg1	natamycin					502:510	the antibiotic natamycin	487:510	the antibiotic natamycin	487:510	As cheese contamination may also occur by undesirable molds, specific actions for preventing their growth are usually realized in dairy industries by using the antibiotic natamycin, which may represent a risk factor for human health and environmental sustainability.
32950139	5	65	dep	3.2 ± 1.8 × 104	1208:1222	arg1	to					1205:1206	to	1205:1206	to	1205:1206	The use of chitosan on cheese rinds did not significantly affect the composition of rind fungal communities developing during the whole ripening process compared with controls treated with natamycin, whose numbers ranged from 3.4 ± 1.3 × 103 to 3.2 ± 1.8 × 104 and from 6.3 ± 3.5 × 102 to 4.0 ± 1.5 × 104, respectively.
32950139	1	66	theme	fungal	123:128	arg1	microbiota					130:139	The fungal microbiota	119:139	The fungal microbiota usually growing on the cheese surface during ripening processes	119:203	The fungal microbiota usually growing on the cheese surface during ripening processes promote rind formation and the development of organoleptic characteristics, imparting positive sensory attributes to cheeses.
32097809	2	0	theme	cellulolytic	563:574	arg1	enzymes					598:604	ligninolytic, cellulolytic, and hemicellulolytic enzymes	549:604	ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group	549:630	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	3	1	theme	cellulose	966:974	arg1	resistance					952:961	the resistance	948:961	the resistance of cellulose and hemicellulose to biodegradation	948:1010	An accurate and a good of fit of the Weibull kinetic model, decreased crystallinity index values, and characteristic absorbance bands in the Fourier transform-infrared spectra further confirmed that cosubstrate addition with FW decreased the resistance of cellulose and hemicellulose to biodegradation.
32097809	5	2	theme	cosubstrate	1215:1225	arg1	addition					1227:1234	The cosubstrate addition	1211:1234	The cosubstrate addition with FW	1211:1242	The cosubstrate addition with FW significantly affected the composition of the ruminal bacteria and fungi in rumen fermentation.
32097809	2	3	theme	ligninolytic	549:560	arg1	enzymes					598:604	ligninolytic, cellulolytic, and hemicellulolytic enzymes	549:604	ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group	549:630	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	1	4	with	groups	319:324	arg1	group					374:378	food waste (FW) group	358:378	food waste (FW) group	358:378	To enhance the degradation of wheat straw (WS) and corn straw (CS) in rumen fermentation, characterization of degradation and ruminal microorganisms of monosubstrate (WS/CS) groups and a cosubstrate strategy with food waste (FW) group was performed.
32097809	1	4	with	groups	319:324	arg1	FW					370:371	FW	370:371	FW	370:371	To enhance the degradation of wheat straw (WS) and corn straw (CS) in rumen fermentation, characterization of degradation and ruminal microorganisms of monosubstrate (WS/CS) groups and a cosubstrate strategy with food waste (FW) group was performed.
32097809	8	5	theme	strategy	1873:1880	arg1	enhancement					1842:1852	an enhancement	1839:1852	an enhancement of the cosubstrate strategy	1839:1880	Moreover, the fermentation properties and rumen flora in the FW rumen fermentation also provided some evidence to suggest an enhancement of the cosubstrate strategy compared with the monosubstrate strategy.
32097809	2	6	theme	enzymes	598:604	arg1	efficiency					448:457	lignin degradation efficiency	429:457	lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group	429:630	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	2	6	theme	enzymes	598:604	arg1	hemicellulose					410:422	hemicellulose	410:422	hemicellulose	410:422	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	2	6	theme	enzymes	598:604	arg1	cellulose					399:407	The cellulose	395:407	The cellulose	395:407	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	5	7	from	composition	1271:1281	arg1	fermentation					1326:1337	rumen fermentation	1320:1337	rumen fermentation	1320:1337	The cosubstrate addition with FW significantly affected the composition of the ruminal bacteria and fungi in rumen fermentation.
32097809	6	8	theme	WS	1548:1549	arg1	fermentation					1557:1568	WS rumen fermentation	1548:1568	WS rumen fermentation with FW supplementation	1548:1592	The relative abundances (RAs) of rumen bacteria were increased in the cosubstrate CS/WS and FW fermentation conditions, and the enhancement of CS degradation with FW supplementation was stronger than that of WS rumen fermentation with FW supplementation.
32097809	4	9	theme	rumen	1152:1156	arg1	fermentation					1158:1169	WS rumen fermentation	1149:1169	WS rumen fermentation	1149:1169	High-throughput sequencing results suggested that the bacterial diversity in CS rumen fermentation and fungal diversity and richness in WS rumen fermentation were promoted with FW as a cosubstrate.
32097809	8	10	from	flora	1765:1769	arg1	fermentation					1787:1798	the FW rumen fermentation	1774:1798	the FW rumen fermentation	1774:1798	Moreover, the fermentation properties and rumen flora in the FW rumen fermentation also provided some evidence to suggest an enhancement of the cosubstrate strategy compared with the monosubstrate strategy.
32097809	8	11	theme	monosubstrate	1900:1912	arg1	strategy					1914:1921	the monosubstrate strategy	1896:1921	the monosubstrate strategy	1896:1921	Moreover, the fermentation properties and rumen flora in the FW rumen fermentation also provided some evidence to suggest an enhancement of the cosubstrate strategy compared with the monosubstrate strategy.
32097809	8	12	theme	fermentation	1731:1742	arg1	properties					1744:1753	fermentation properties	1731:1753	fermentation properties	1731:1753	Moreover, the fermentation properties and rumen flora in the FW rumen fermentation also provided some evidence to suggest an enhancement of the cosubstrate strategy compared with the monosubstrate strategy.
32097809	1	13	theme	corn	196:199	arg1	CS					208:209	CS	208:209	CS	208:209	To enhance the degradation of wheat straw (WS) and corn straw (CS) in rumen fermentation, characterization of degradation and ruminal microorganisms of monosubstrate (WS/CS) groups and a cosubstrate strategy with food waste (FW) group was performed.
32097809	1	13	theme	corn	196:199	arg1	straw					201:205	corn straw	196:205	corn straw (CS)	196:210	To enhance the degradation of wheat straw (WS) and corn straw (CS) in rumen fermentation, characterization of degradation and ruminal microorganisms of monosubstrate (WS/CS) groups and a cosubstrate strategy with food waste (FW) group was performed.
32097809	2	14	theme	acid	520:523	arg1	yields					525:530	volatile fatty acid yields	505:530	volatile fatty acid yields	505:530	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	4	15	theme	fungal	1116:1121	arg1	diversity					1077:1085	the bacterial diversity	1063:1085	the bacterial diversity in CS rumen fermentation	1063:1110	High-throughput sequencing results suggested that the bacterial diversity in CS rumen fermentation and fungal diversity and richness in WS rumen fermentation were promoted with FW as a cosubstrate.
32097809	4	15	theme	fungal	1116:1121	arg1	cosubstrate					1198:1208	a cosubstrate	1196:1208	a cosubstrate	1196:1208	High-throughput sequencing results suggested that the bacterial diversity in CS rumen fermentation and fungal diversity and richness in WS rumen fermentation were promoted with FW as a cosubstrate.
32097809	4	15	theme	fungal	1116:1121	arg1	diversity					1123:1131	diversity	1123:1131	diversity	1123:1131	High-throughput sequencing results suggested that the bacterial diversity in CS rumen fermentation and fungal diversity and richness in WS rumen fermentation were promoted with FW as a cosubstrate.
32097809	7	16	theme	Fusarium	1645:1652	arg1	RAs					1599:1601	The RAs	1595:1601	The RAs of the ruminal fungal genera Ustilago and Fusarium	1595:1652	The RAs of the ruminal fungal genera Ustilago and Fusarium were promoted in CS and WS fermentation with FW, respectively.
32097809	2	17	theme	volatile	505:512	arg1	yields					525:530	volatile fatty acid yields	505:530	volatile fatty acid yields	505:530	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	3	18	theme	characteristic	812:825	arg1	bands					838:842	characteristic absorbance bands	812:842	characteristic absorbance bands	812:842	An accurate and a good of fit of the Weibull kinetic model, decreased crystallinity index values, and characteristic absorbance bands in the Fourier transform-infrared spectra further confirmed that cosubstrate addition with FW decreased the resistance of cellulose and hemicellulose to biodegradation.
32097809	6	19	theme	relative	1344:1351	arg1	abundances					1353:1362	The relative abundances	1340:1362	The relative abundances (RAs) of rumen bacteria	1340:1386	The relative abundances (RAs) of rumen bacteria were increased in the cosubstrate CS/WS and FW fermentation conditions, and the enhancement of CS degradation with FW supplementation was stronger than that of WS rumen fermentation with FW supplementation.
32097809	6	19	theme	relative	1344:1351	arg1	RAs					1365:1367	RAs	1365:1367	RAs	1365:1367	The relative abundances (RAs) of rumen bacteria were increased in the cosubstrate CS/WS and FW fermentation conditions, and the enhancement of CS degradation with FW supplementation was stronger than that of WS rumen fermentation with FW supplementation.
32097809	3	20	with	addition	921:928	arg1	FW					935:936	FW	935:936	FW	935:936	An accurate and a good of fit of the Weibull kinetic model, decreased crystallinity index values, and characteristic absorbance bands in the Fourier transform-infrared spectra further confirmed that cosubstrate addition with FW decreased the resistance of cellulose and hemicellulose to biodegradation.
32097809	7	21	theme	Ustilago	1632:1639	arg1	RAs					1599:1601	The RAs	1595:1601	The RAs of the ruminal fungal genera Ustilago and Fusarium	1595:1652	The RAs of the ruminal fungal genera Ustilago and Fusarium were promoted in CS and WS fermentation with FW, respectively.
32097809	2	22	theme	activity	537:544	arg1	efficiency					448:457	lignin degradation efficiency	429:457	lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group	429:630	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	2	22	theme	activity	537:544	arg1	hemicellulose					410:422	hemicellulose	410:422	hemicellulose	410:422	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	2	22	theme	activity	537:544	arg1	cellulose					399:407	The cellulose	395:407	The cellulose	395:407	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	2	23	dep	improved	637:644	arg1	compared					646:653	compared	646:653	compared with those for the corresponding monosubstrate groups	646:707	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	7	24	theme	fungal	1618:1623	arg1	Ustilago					1632:1639	the ruminal fungal genera Ustilago and Fusarium	1606:1652	Ustilago	1632:1639	The RAs of the ruminal fungal genera Ustilago and Fusarium were promoted in CS and WS fermentation with FW, respectively.
32097809	3	25	theme	model	763:767	arg1	fit					736:738	fit	736:738	fit of the Weibull kinetic model	736:767	An accurate and a good of fit of the Weibull kinetic model, decreased crystallinity index values, and characteristic absorbance bands in the Fourier transform-infrared spectra further confirmed that cosubstrate addition with FW decreased the resistance of cellulose and hemicellulose to biodegradation.
32097809	3	25	theme	model	763:767	arg1	bands					838:842	characteristic absorbance bands	812:842	characteristic absorbance bands	812:842	An accurate and a good of fit of the Weibull kinetic model, decreased crystallinity index values, and characteristic absorbance bands in the Fourier transform-infrared spectra further confirmed that cosubstrate addition with FW decreased the resistance of cellulose and hemicellulose to biodegradation.
32097809	3	25	theme	model	763:767	arg1	values					800:805	decreased crystallinity index values	770:805	decreased crystallinity index values	770:805	An accurate and a good of fit of the Weibull kinetic model, decreased crystallinity index values, and characteristic absorbance bands in the Fourier transform-infrared spectra further confirmed that cosubstrate addition with FW decreased the resistance of cellulose and hemicellulose to biodegradation.
32097809	8	26	theme	rumen	1781:1785	arg1	fermentation					1787:1798	the FW rumen fermentation	1774:1798	the FW rumen fermentation	1774:1798	Moreover, the fermentation properties and rumen flora in the FW rumen fermentation also provided some evidence to suggest an enhancement of the cosubstrate strategy compared with the monosubstrate strategy.
32097809	3	27	theme	Weibull	747:753	arg1	model					763:767	the Weibull kinetic model	743:767	the Weibull kinetic model	743:767	An accurate and a good of fit of the Weibull kinetic model, decreased crystallinity index values, and characteristic absorbance bands in the Fourier transform-infrared spectra further confirmed that cosubstrate addition with FW decreased the resistance of cellulose and hemicellulose to biodegradation.
32097809	0	28	theme	fermentation	75:86	arg1	in vitro					88:95	rumen fermentation in vitro	69:95	rumen fermentation in vitro	69:95	Cosubstrate strategy for enhancing lignocellulose degradation during rumen fermentation in vitro: Characteristics and microorganism composition.
32097809	6	29	theme	FW	1575:1576	arg1	supplementation					1578:1592	FW supplementation	1575:1592	FW supplementation	1575:1592	The relative abundances (RAs) of rumen bacteria were increased in the cosubstrate CS/WS and FW fermentation conditions, and the enhancement of CS degradation with FW supplementation was stronger than that of WS rumen fermentation with FW supplementation.
32097809	4	30	from	diversity	1077:1085	arg1	fermentation					1099:1110	CS rumen fermentation	1090:1110	CS rumen fermentation	1090:1110	High-throughput sequencing results suggested that the bacterial diversity in CS rumen fermentation and fungal diversity and richness in WS rumen fermentation were promoted with FW as a cosubstrate.
32097809	4	30	from	diversity	1077:1085	arg1	fermentation					1158:1169	WS rumen fermentation	1149:1169	WS rumen fermentation	1149:1169	High-throughput sequencing results suggested that the bacterial diversity in CS rumen fermentation and fungal diversity and richness in WS rumen fermentation were promoted with FW as a cosubstrate.
32097809	2	31	theme	monosubstrate	688:700	arg1	groups					702:707	the corresponding monosubstrate groups	670:707	the corresponding monosubstrate groups	670:707	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	3	32	theme	index	794:798	arg1	values					800:805	decreased crystallinity index values	770:805	decreased crystallinity index values	770:805	An accurate and a good of fit of the Weibull kinetic model, decreased crystallinity index values, and characteristic absorbance bands in the Fourier transform-infrared spectra further confirmed that cosubstrate addition with FW decreased the resistance of cellulose and hemicellulose to biodegradation.
32097809	1	33	theme	cosubstrate	332:342	arg1	strategy					344:351	a cosubstrate strategy	330:351	a cosubstrate strategy with food waste (FW) group	330:378	To enhance the degradation of wheat straw (WS) and corn straw (CS) in rumen fermentation, characterization of degradation and ruminal microorganisms of monosubstrate (WS/CS) groups and a cosubstrate strategy with food waste (FW) group was performed.
32097809	3	34	theme	bands	838:842	arg1	accurate					713:720	accurate	713:720	accurate	713:720	An accurate and a good of fit of the Weibull kinetic model, decreased crystallinity index values, and characteristic absorbance bands in the Fourier transform-infrared spectra further confirmed that cosubstrate addition with FW decreased the resistance of cellulose and hemicellulose to biodegradation.
32097809	2	35	theme	demand	497:502	arg1	efficiency					448:457	lignin degradation efficiency	429:457	lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group	429:630	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	2	35	theme	demand	497:502	arg1	hemicellulose					410:422	hemicellulose	410:422	hemicellulose	410:422	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	2	35	theme	demand	497:502	arg1	cellulose					399:407	The cellulose	395:407	The cellulose	395:407	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	2	36	theme	cosubstrate	614:624	arg1	group					626:630	the cosubstrate group	610:630	the cosubstrate group	610:630	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	5	37	theme	ruminal	1290:1296	arg1	bacteria					1298:1305	the ruminal bacteria	1286:1305	the ruminal bacteria	1286:1305	The cosubstrate addition with FW significantly affected the composition of the ruminal bacteria and fungi in rumen fermentation.
32097809	3	38	theme	decreased	770:778	arg1	values					800:805	decreased crystallinity index values	770:805	decreased crystallinity index values	770:805	An accurate and a good of fit of the Weibull kinetic model, decreased crystallinity index values, and characteristic absorbance bands in the Fourier transform-infrared spectra further confirmed that cosubstrate addition with FW decreased the resistance of cellulose and hemicellulose to biodegradation.
32097809	2	39	theme	chemical	481:488	arg1	demand					497:502	soluble chemical oxygen demand	473:502	soluble chemical oxygen demand	473:502	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	3	40	from	accurate	713:720	arg1	spectra					878:884	the Fourier transform-infrared spectra	847:884	the Fourier transform-infrared spectra	847:884	An accurate and a good of fit of the Weibull kinetic model, decreased crystallinity index values, and characteristic absorbance bands in the Fourier transform-infrared spectra further confirmed that cosubstrate addition with FW decreased the resistance of cellulose and hemicellulose to biodegradation.
32097809	1	41	theme	waste	363:367	arg1	group					374:378	food waste (FW) group	358:378	food waste (FW) group	358:378	To enhance the degradation of wheat straw (WS) and corn straw (CS) in rumen fermentation, characterization of degradation and ruminal microorganisms of monosubstrate (WS/CS) groups and a cosubstrate strategy with food waste (FW) group was performed.
32097809	1	41	theme	waste	363:367	arg1	FW					370:371	FW	370:371	FW	370:371	To enhance the degradation of wheat straw (WS) and corn straw (CS) in rumen fermentation, characterization of degradation and ruminal microorganisms of monosubstrate (WS/CS) groups and a cosubstrate strategy with food waste (FW) group was performed.
32097809	3	42	theme	transform-infrared	859:876	arg1	spectra					878:884	the Fourier transform-infrared spectra	847:884	the Fourier transform-infrared spectra	847:884	An accurate and a good of fit of the Weibull kinetic model, decreased crystallinity index values, and characteristic absorbance bands in the Fourier transform-infrared spectra further confirmed that cosubstrate addition with FW decreased the resistance of cellulose and hemicellulose to biodegradation.
32097809	0	43	theme	Cosubstrate	0:10	arg1	strategy					12:19	Cosubstrate strategy	0:19	Cosubstrate strategy for enhancing lignocellulose degradation during rumen fermentation in vitro	0:95	Cosubstrate strategy for enhancing lignocellulose degradation during rumen fermentation in vitro: Characteristics and microorganism composition.
32097809	1	44	theme	ruminal	271:277	arg1	microorganisms					279:292	ruminal microorganisms	271:292	ruminal microorganisms	271:292	To enhance the degradation of wheat straw (WS) and corn straw (CS) in rumen fermentation, characterization of degradation and ruminal microorganisms of monosubstrate (WS/CS) groups and a cosubstrate strategy with food waste (FW) group was performed.
32097809	6	45	theme	fermentation	1435:1446	arg1	conditions					1448:1457	FW fermentation conditions	1432:1457	FW fermentation conditions	1432:1457	The relative abundances (RAs) of rumen bacteria were increased in the cosubstrate CS/WS and FW fermentation conditions, and the enhancement of CS degradation with FW supplementation was stronger than that of WS rumen fermentation with FW supplementation.
32097809	7	46	theme	CS	1671:1672	arg1	fermentation					1681:1692	CS and WS fermentation	1671:1692	CS and WS fermentation with FW	1671:1700	The RAs of the ruminal fungal genera Ustilago and Fusarium were promoted in CS and WS fermentation with FW, respectively.
32097809	0	47	theme	lignocellulose	35:48	arg1	degradation					50:60	lignocellulose degradation	35:60	lignocellulose degradation	35:60	Cosubstrate strategy for enhancing lignocellulose degradation during rumen fermentation in vitro: Characteristics and microorganism composition.
32097809	4	48	theme	High-throughput	1013:1027	arg1	results					1040:1046	High-throughput sequencing results	1013:1046	High-throughput sequencing results	1013:1046	High-throughput sequencing results suggested that the bacterial diversity in CS rumen fermentation and fungal diversity and richness in WS rumen fermentation were promoted with FW as a cosubstrate.
32097809	6	49	theme	cosubstrate	1410:1420	arg1	CS/WS					1422:1426	the cosubstrate CS/WS	1406:1426	the cosubstrate CS/WS	1406:1426	The relative abundances (RAs) of rumen bacteria were increased in the cosubstrate CS/WS and FW fermentation conditions, and the enhancement of CS degradation with FW supplementation was stronger than that of WS rumen fermentation with FW supplementation.
32097809	4	50	theme	rumen	1093:1097	arg1	fermentation					1099:1110	CS rumen fermentation	1090:1110	CS rumen fermentation	1090:1110	High-throughput sequencing results suggested that the bacterial diversity in CS rumen fermentation and fungal diversity and richness in WS rumen fermentation were promoted with FW as a cosubstrate.
32097809	8	51	theme	FW	1778:1779	arg1	fermentation					1787:1798	the FW rumen fermentation	1774:1798	the FW rumen fermentation	1774:1798	Moreover, the fermentation properties and rumen flora in the FW rumen fermentation also provided some evidence to suggest an enhancement of the cosubstrate strategy compared with the monosubstrate strategy.
32097809	6	52	theme	FW	1503:1504	arg1	supplementation					1506:1520	FW supplementation	1503:1520	FW supplementation	1503:1520	The relative abundances (RAs) of rumen bacteria were increased in the cosubstrate CS/WS and FW fermentation conditions, and the enhancement of CS degradation with FW supplementation was stronger than that of WS rumen fermentation with FW supplementation.
32097809	1	53	theme	wheat	175:179	arg1	WS					188:189	WS	188:189	WS	188:189	To enhance the degradation of wheat straw (WS) and corn straw (CS) in rumen fermentation, characterization of degradation and ruminal microorganisms of monosubstrate (WS/CS) groups and a cosubstrate strategy with food waste (FW) group was performed.
32097809	1	53	theme	wheat	175:179	arg1	straw					181:185	wheat straw	175:185	wheat straw (WS)	175:190	To enhance the degradation of wheat straw (WS) and corn straw (CS) in rumen fermentation, characterization of degradation and ruminal microorganisms of monosubstrate (WS/CS) groups and a cosubstrate strategy with food waste (FW) group was performed.
32097809	6	54	theme	degradation	1486:1496	arg1	enhancement					1468:1478	the enhancement	1464:1478	the enhancement of CS degradation with FW supplementation	1464:1520	The relative abundances (RAs) of rumen bacteria were increased in the cosubstrate CS/WS and FW fermentation conditions, and the enhancement of CS degradation with FW supplementation was stronger than that of WS rumen fermentation with FW supplementation.
32097809	6	54	theme	degradation	1486:1496	arg1	stronger					1526:1533	stronger	1526:1533	stronger	1526:1533	The relative abundances (RAs) of rumen bacteria were increased in the cosubstrate CS/WS and FW fermentation conditions, and the enhancement of CS degradation with FW supplementation was stronger than that of WS rumen fermentation with FW supplementation.
32097809	2	55	theme	degradation	436:446	arg1	efficiency					448:457	lignin degradation efficiency	429:457	lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group	429:630	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	4	56	theme	bacterial	1067:1075	arg1	diversity					1077:1085	the bacterial diversity	1063:1085	the bacterial diversity in CS rumen fermentation	1063:1110	High-throughput sequencing results suggested that the bacterial diversity in CS rumen fermentation and fungal diversity and richness in WS rumen fermentation were promoted with FW as a cosubstrate.
32097809	4	56	theme	bacterial	1067:1075	arg1	cosubstrate					1198:1208	a cosubstrate	1196:1208	a cosubstrate	1196:1208	High-throughput sequencing results suggested that the bacterial diversity in CS rumen fermentation and fungal diversity and richness in WS rumen fermentation were promoted with FW as a cosubstrate.
32097809	4	56	theme	bacterial	1067:1075	arg1	diversity					1123:1131	diversity	1123:1131	diversity	1123:1131	High-throughput sequencing results suggested that the bacterial diversity in CS rumen fermentation and fungal diversity and richness in WS rumen fermentation were promoted with FW as a cosubstrate.
32097809	8	57	theme	cosubstrate	1861:1871	arg1	strategy					1873:1880	the cosubstrate strategy	1857:1880	the cosubstrate strategy	1857:1880	Moreover, the fermentation properties and rumen flora in the FW rumen fermentation also provided some evidence to suggest an enhancement of the cosubstrate strategy compared with the monosubstrate strategy.
32097809	1	58	theme	degradation	255:265	arg1	characterization					235:250	characterization	235:250	characterization of degradation and ruminal microorganisms of monosubstrate (WS/CS) groups and a cosubstrate strategy with food waste (FW) group	235:378	To enhance the degradation of wheat straw (WS) and corn straw (CS) in rumen fermentation, characterization of degradation and ruminal microorganisms of monosubstrate (WS/CS) groups and a cosubstrate strategy with food waste (FW) group was performed.
32097809	4	59	theme	WS	1149:1150	arg1	fermentation					1158:1169	WS rumen fermentation	1149:1169	WS rumen fermentation	1149:1169	High-throughput sequencing results suggested that the bacterial diversity in CS rumen fermentation and fungal diversity and richness in WS rumen fermentation were promoted with FW as a cosubstrate.
32097809	6	60	with	enhancement	1468:1478	arg1	supplementation					1506:1520	FW supplementation	1503:1520	FW supplementation	1503:1520	The relative abundances (RAs) of rumen bacteria were increased in the cosubstrate CS/WS and FW fermentation conditions, and the enhancement of CS degradation with FW supplementation was stronger than that of WS rumen fermentation with FW supplementation.
32097809	3	61	theme	Fourier	851:857	arg1	spectra					878:884	the Fourier transform-infrared spectra	847:884	the Fourier transform-infrared spectra	847:884	An accurate and a good of fit of the Weibull kinetic model, decreased crystallinity index values, and characteristic absorbance bands in the Fourier transform-infrared spectra further confirmed that cosubstrate addition with FW decreased the resistance of cellulose and hemicellulose to biodegradation.
32097809	6	62	theme	rumen	1551:1555	arg1	fermentation					1557:1568	WS rumen fermentation	1548:1568	WS rumen fermentation with FW supplementation	1548:1592	The relative abundances (RAs) of rumen bacteria were increased in the cosubstrate CS/WS and FW fermentation conditions, and the enhancement of CS degradation with FW supplementation was stronger than that of WS rumen fermentation with FW supplementation.
32097809	2	63	theme	hemicellulolytic	581:596	arg1	enzymes					598:604	ligninolytic, cellulolytic, and hemicellulolytic enzymes	549:604	ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group	549:630	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	1	64	theme	straw	201:205	arg1	degradation					160:170	the degradation	156:170	the degradation of wheat straw (WS) and corn straw (CS) in rumen fermentation	156:232	To enhance the degradation of wheat straw (WS) and corn straw (CS) in rumen fermentation, characterization of degradation and ruminal microorganisms of monosubstrate (WS/CS) groups and a cosubstrate strategy with food waste (FW) group was performed.
32097809	2	65	theme	fatty	514:518	arg1	yields					525:530	volatile fatty acid yields	505:530	volatile fatty acid yields	505:530	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	6	66	with	fermentation	1557:1568	arg1	supplementation					1578:1592	FW supplementation	1575:1592	FW supplementation	1575:1592	The relative abundances (RAs) of rumen bacteria were increased in the cosubstrate CS/WS and FW fermentation conditions, and the enhancement of CS degradation with FW supplementation was stronger than that of WS rumen fermentation with FW supplementation.
32097809	4	67	from	diversity	1123:1131	arg1	fermentation					1099:1110	CS rumen fermentation	1090:1110	CS rumen fermentation	1090:1110	High-throughput sequencing results suggested that the bacterial diversity in CS rumen fermentation and fungal diversity and richness in WS rumen fermentation were promoted with FW as a cosubstrate.
32097809	4	67	from	diversity	1123:1131	arg1	fermentation					1158:1169	WS rumen fermentation	1149:1169	WS rumen fermentation	1149:1169	High-throughput sequencing results suggested that the bacterial diversity in CS rumen fermentation and fungal diversity and richness in WS rumen fermentation were promoted with FW as a cosubstrate.
32097809	7	68	theme	genera	1625:1630	arg1	Ustilago					1632:1639	the ruminal fungal genera Ustilago and Fusarium	1606:1652	Ustilago	1632:1639	The RAs of the ruminal fungal genera Ustilago and Fusarium were promoted in CS and WS fermentation with FW, respectively.
32097809	2	69	theme	corresponding	674:686	arg1	groups					702:707	the corresponding monosubstrate groups	670:707	the corresponding monosubstrate groups	670:707	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	6	70	theme	bacteria	1379:1386	arg1	abundances					1353:1362	The relative abundances	1340:1362	The relative abundances (RAs) of rumen bacteria	1340:1386	The relative abundances (RAs) of rumen bacteria were increased in the cosubstrate CS/WS and FW fermentation conditions, and the enhancement of CS degradation with FW supplementation was stronger than that of WS rumen fermentation with FW supplementation.
32097809	6	70	theme	bacteria	1379:1386	arg1	RAs					1365:1367	RAs	1365:1367	RAs	1365:1367	The relative abundances (RAs) of rumen bacteria were increased in the cosubstrate CS/WS and FW fermentation conditions, and the enhancement of CS degradation with FW supplementation was stronger than that of WS rumen fermentation with FW supplementation.
32097809	8	71	theme	rumen	1759:1763	arg1	flora					1765:1769	rumen flora	1759:1769	rumen flora	1759:1769	Moreover, the fermentation properties and rumen flora in the FW rumen fermentation also provided some evidence to suggest an enhancement of the cosubstrate strategy compared with the monosubstrate strategy.
32097809	7	72	theme	ruminal	1610:1616	arg1	Ustilago					1632:1639	the ruminal fungal genera Ustilago and Fusarium	1606:1652	Ustilago	1632:1639	The RAs of the ruminal fungal genera Ustilago and Fusarium were promoted in CS and WS fermentation with FW, respectively.
32097809	7	73	with	fermentation	1681:1692	arg1	FW					1699:1700	FW	1699:1700	FW	1699:1700	The RAs of the ruminal fungal genera Ustilago and Fusarium were promoted in CS and WS fermentation with FW, respectively.
32097809	5	74	theme	bacteria	1298:1305	arg1	composition					1271:1281	the composition	1267:1281	the composition of the ruminal bacteria and fungi in rumen fermentation	1267:1337	The cosubstrate addition with FW significantly affected the composition of the ruminal bacteria and fungi in rumen fermentation.
32097809	3	75	theme	fit	736:738	arg1	accurate					713:720	accurate	713:720	accurate	713:720	An accurate and a good of fit of the Weibull kinetic model, decreased crystallinity index values, and characteristic absorbance bands in the Fourier transform-infrared spectra further confirmed that cosubstrate addition with FW decreased the resistance of cellulose and hemicellulose to biodegradation.
32097809	8	76	dep	properties	1744:1753	arg1	the					1727:1729	the	1727:1729	the	1727:1729	Moreover, the fermentation properties and rumen flora in the FW rumen fermentation also provided some evidence to suggest an enhancement of the cosubstrate strategy compared with the monosubstrate strategy.
32097809	3	77	theme	absorbance	827:836	arg1	bands					838:842	characteristic absorbance bands	812:842	characteristic absorbance bands	812:842	An accurate and a good of fit of the Weibull kinetic model, decreased crystallinity index values, and characteristic absorbance bands in the Fourier transform-infrared spectra further confirmed that cosubstrate addition with FW decreased the resistance of cellulose and hemicellulose to biodegradation.
32097809	2	78	theme	yields	525:530	arg1	efficiency					448:457	lignin degradation efficiency	429:457	lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group	429:630	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	2	78	theme	yields	525:530	arg1	hemicellulose					410:422	hemicellulose	410:422	hemicellulose	410:422	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	2	78	theme	yields	525:530	arg1	cellulose					399:407	The cellulose	395:407	The cellulose	395:407	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	3	79	from	spectra	878:884	arg1	accurate					713:720	accurate	713:720	accurate	713:720	An accurate and a good of fit of the Weibull kinetic model, decreased crystallinity index values, and characteristic absorbance bands in the Fourier transform-infrared spectra further confirmed that cosubstrate addition with FW decreased the resistance of cellulose and hemicellulose to biodegradation.
32097809	5	80	theme	fungi	1311:1315	arg1	composition					1271:1281	the composition	1267:1281	the composition of the ruminal bacteria and fungi in rumen fermentation	1267:1337	The cosubstrate addition with FW significantly affected the composition of the ruminal bacteria and fungi in rumen fermentation.
32097809	4	81	from	richness	1137:1144	arg1	fermentation					1099:1110	CS rumen fermentation	1090:1110	CS rumen fermentation	1090:1110	High-throughput sequencing results suggested that the bacterial diversity in CS rumen fermentation and fungal diversity and richness in WS rumen fermentation were promoted with FW as a cosubstrate.
32097809	4	81	from	richness	1137:1144	arg1	fermentation					1158:1169	WS rumen fermentation	1149:1169	WS rumen fermentation	1149:1169	High-throughput sequencing results suggested that the bacterial diversity in CS rumen fermentation and fungal diversity and richness in WS rumen fermentation were promoted with FW as a cosubstrate.
32097809	2	82	theme	CS	469:470	arg1	efficiency					448:457	lignin degradation efficiency	429:457	lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group	429:630	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	2	82	theme	CS	469:470	arg1	hemicellulose					410:422	hemicellulose	410:422	hemicellulose	410:422	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	2	82	theme	CS	469:470	arg1	cellulose					399:407	The cellulose	395:407	The cellulose	395:407	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	3	83	theme	kinetic	755:761	arg1	model					763:767	the Weibull kinetic model	743:767	the Weibull kinetic model	743:767	An accurate and a good of fit of the Weibull kinetic model, decreased crystallinity index values, and characteristic absorbance bands in the Fourier transform-infrared spectra further confirmed that cosubstrate addition with FW decreased the resistance of cellulose and hemicellulose to biodegradation.
32097809	1	84	theme	straw	181:185	arg1	degradation					160:170	the degradation	156:170	the degradation of wheat straw (WS) and corn straw (CS) in rumen fermentation	156:232	To enhance the degradation of wheat straw (WS) and corn straw (CS) in rumen fermentation, characterization of degradation and ruminal microorganisms of monosubstrate (WS/CS) groups and a cosubstrate strategy with food waste (FW) group was performed.
32097809	1	85	with	strategy	344:351	arg1	group					374:378	food waste (FW) group	358:378	food waste (FW) group	358:378	To enhance the degradation of wheat straw (WS) and corn straw (CS) in rumen fermentation, characterization of degradation and ruminal microorganisms of monosubstrate (WS/CS) groups and a cosubstrate strategy with food waste (FW) group was performed.
32097809	1	85	with	strategy	344:351	arg1	FW					370:371	FW	370:371	FW	370:371	To enhance the degradation of wheat straw (WS) and corn straw (CS) in rumen fermentation, characterization of degradation and ruminal microorganisms of monosubstrate (WS/CS) groups and a cosubstrate strategy with food waste (FW) group was performed.
32097809	2	86	theme	WS	462:463	arg1	efficiency					448:457	lignin degradation efficiency	429:457	lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group	429:630	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	2	86	theme	WS	462:463	arg1	hemicellulose					410:422	hemicellulose	410:422	hemicellulose	410:422	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	2	86	theme	WS	462:463	arg1	cellulose					399:407	The cellulose	395:407	The cellulose	395:407	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	3	87	theme	values	800:805	arg1	accurate					713:720	accurate	713:720	accurate	713:720	An accurate and a good of fit of the Weibull kinetic model, decreased crystallinity index values, and characteristic absorbance bands in the Fourier transform-infrared spectra further confirmed that cosubstrate addition with FW decreased the resistance of cellulose and hemicellulose to biodegradation.
32097809	0	88	theme	microorganism	118:130	arg1	composition					132:142	microorganism composition	118:142	microorganism composition	118:142	Cosubstrate strategy for enhancing lignocellulose degradation during rumen fermentation in vitro: Characteristics and microorganism composition.
32097809	1	89	theme	strategy	344:351	arg1	characterization					235:250	characterization	235:250	characterization of degradation and ruminal microorganisms of monosubstrate (WS/CS) groups and a cosubstrate strategy with food waste (FW) group	235:378	To enhance the degradation of wheat straw (WS) and corn straw (CS) in rumen fermentation, characterization of degradation and ruminal microorganisms of monosubstrate (WS/CS) groups and a cosubstrate strategy with food waste (FW) group was performed.
32097809	2	90	theme	oxygen	490:495	arg1	demand					497:502	soluble chemical oxygen demand	473:502	soluble chemical oxygen demand	473:502	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	3	91	theme	crystallinity	780:792	arg1	values					800:805	decreased crystallinity index values	770:805	decreased crystallinity index values	770:805	An accurate and a good of fit of the Weibull kinetic model, decreased crystallinity index values, and characteristic absorbance bands in the Fourier transform-infrared spectra further confirmed that cosubstrate addition with FW decreased the resistance of cellulose and hemicellulose to biodegradation.
32097809	1	92	theme	food	358:361	arg1	group					374:378	food waste (FW) group	358:378	food waste (FW) group	358:378	To enhance the degradation of wheat straw (WS) and corn straw (CS) in rumen fermentation, characterization of degradation and ruminal microorganisms of monosubstrate (WS/CS) groups and a cosubstrate strategy with food waste (FW) group was performed.
32097809	1	92	theme	food	358:361	arg1	FW					370:371	FW	370:371	FW	370:371	To enhance the degradation of wheat straw (WS) and corn straw (CS) in rumen fermentation, characterization of degradation and ruminal microorganisms of monosubstrate (WS/CS) groups and a cosubstrate strategy with food waste (FW) group was performed.
32097809	2	93	theme	soluble	473:479	arg1	demand					497:502	soluble chemical oxygen demand	473:502	soluble chemical oxygen demand	473:502	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	1	94	from	degradation	160:170	arg1	fermentation					221:232	rumen fermentation	215:232	rumen fermentation	215:232	To enhance the degradation of wheat straw (WS) and corn straw (CS) in rumen fermentation, characterization of degradation and ruminal microorganisms of monosubstrate (WS/CS) groups and a cosubstrate strategy with food waste (FW) group was performed.
32097809	0	95	dep	Characteristics	98:112	arg1	strategy					12:19	Cosubstrate strategy	0:19	Cosubstrate strategy for enhancing lignocellulose degradation during rumen fermentation in vitro	0:95	Cosubstrate strategy for enhancing lignocellulose degradation during rumen fermentation in vitro: Characteristics and microorganism composition.
32097809	4	96	theme	sequencing	1029:1038	arg1	results					1040:1046	High-throughput sequencing results	1013:1046	High-throughput sequencing results	1013:1046	High-throughput sequencing results suggested that the bacterial diversity in CS rumen fermentation and fungal diversity and richness in WS rumen fermentation were promoted with FW as a cosubstrate.
32097809	5	97	theme	rumen	1320:1324	arg1	fermentation					1326:1337	rumen fermentation	1320:1337	rumen fermentation	1320:1337	The cosubstrate addition with FW significantly affected the composition of the ruminal bacteria and fungi in rumen fermentation.
32097809	1	98	theme	microorganisms	279:292	arg1	characterization					235:250	characterization	235:250	characterization of degradation and ruminal microorganisms of monosubstrate (WS/CS) groups and a cosubstrate strategy with food waste (FW) group	235:378	To enhance the degradation of wheat straw (WS) and corn straw (CS) in rumen fermentation, characterization of degradation and ruminal microorganisms of monosubstrate (WS/CS) groups and a cosubstrate strategy with food waste (FW) group was performed.
32097809	7	99	theme	WS	1678:1679	arg1	fermentation					1681:1692	CS and WS fermentation	1671:1692	CS and WS fermentation with FW	1671:1700	The RAs of the ruminal fungal genera Ustilago and Fusarium were promoted in CS and WS fermentation with FW, respectively.
32097809	6	100	theme	FW	1432:1433	arg1	conditions					1448:1457	FW fermentation conditions	1432:1457	FW fermentation conditions	1432:1457	The relative abundances (RAs) of rumen bacteria were increased in the cosubstrate CS/WS and FW fermentation conditions, and the enhancement of CS degradation with FW supplementation was stronger than that of WS rumen fermentation with FW supplementation.
32097809	1	101	theme	monosubstrate	297:309	arg1	groups					319:324	monosubstrate (WS/CS) groups	297:324	monosubstrate (WS/CS) groups	297:324	To enhance the degradation of wheat straw (WS) and corn straw (CS) in rumen fermentation, characterization of degradation and ruminal microorganisms of monosubstrate (WS/CS) groups and a cosubstrate strategy with food waste (FW) group was performed.
32097809	3	102	theme	cosubstrate	909:919	arg1	addition					921:928	cosubstrate addition	909:928	cosubstrate addition with FW	909:936	An accurate and a good of fit of the Weibull kinetic model, decreased crystallinity index values, and characteristic absorbance bands in the Fourier transform-infrared spectra further confirmed that cosubstrate addition with FW decreased the resistance of cellulose and hemicellulose to biodegradation.
32097809	5	103	with	addition	1227:1234	arg1	FW					1241:1242	FW	1241:1242	FW	1241:1242	The cosubstrate addition with FW significantly affected the composition of the ruminal bacteria and fungi in rumen fermentation.
32097809	0	104	theme	rumen	69:73	arg1	in vitro					88:95	rumen fermentation in vitro	69:95	rumen fermentation in vitro	69:95	Cosubstrate strategy for enhancing lignocellulose degradation during rumen fermentation in vitro: Characteristics and microorganism composition.
32097809	1	105	theme	WS/CS	312:316	arg1	groups					319:324	monosubstrate (WS/CS) groups	297:324	monosubstrate (WS/CS) groups	297:324	To enhance the degradation of wheat straw (WS) and corn straw (CS) in rumen fermentation, characterization of degradation and ruminal microorganisms of monosubstrate (WS/CS) groups and a cosubstrate strategy with food waste (FW) group was performed.
32097809	2	106	theme	lignin	429:434	arg1	efficiency					448:457	lignin degradation efficiency	429:457	lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group	429:630	The cellulose, hemicellulose, and lignin degradation efficiency of WS and CS; soluble chemical oxygen demand; volatile fatty acid yields; and activity of ligninolytic, cellulolytic, and hemicellulolytic enzymes for the cosubstrate group were improved compared with those for the corresponding monosubstrate groups.
32097809	1	107	theme	groups	319:324	arg1	characterization					235:250	characterization	235:250	characterization of degradation and ruminal microorganisms of monosubstrate (WS/CS) groups and a cosubstrate strategy with food waste (FW) group	235:378	To enhance the degradation of wheat straw (WS) and corn straw (CS) in rumen fermentation, characterization of degradation and ruminal microorganisms of monosubstrate (WS/CS) groups and a cosubstrate strategy with food waste (FW) group was performed.
32097809	4	108	theme	CS	1090:1091	arg1	fermentation					1099:1110	CS rumen fermentation	1090:1110	CS rumen fermentation	1090:1110	High-throughput sequencing results suggested that the bacterial diversity in CS rumen fermentation and fungal diversity and richness in WS rumen fermentation were promoted with FW as a cosubstrate.
32097809	8	109	from	properties	1744:1753	arg1	fermentation					1787:1798	the FW rumen fermentation	1774:1798	the FW rumen fermentation	1774:1798	Moreover, the fermentation properties and rumen flora in the FW rumen fermentation also provided some evidence to suggest an enhancement of the cosubstrate strategy compared with the monosubstrate strategy.
32097809	1	110	theme	rumen	215:219	arg1	fermentation					221:232	rumen fermentation	215:232	rumen fermentation	215:232	To enhance the degradation of wheat straw (WS) and corn straw (CS) in rumen fermentation, characterization of degradation and ruminal microorganisms of monosubstrate (WS/CS) groups and a cosubstrate strategy with food waste (FW) group was performed.
32097809	3	111	theme	hemicellulose	980:992	arg1	resistance					952:961	the resistance	948:961	the resistance of cellulose and hemicellulose to biodegradation	948:1010	An accurate and a good of fit of the Weibull kinetic model, decreased crystallinity index values, and characteristic absorbance bands in the Fourier transform-infrared spectra further confirmed that cosubstrate addition with FW decreased the resistance of cellulose and hemicellulose to biodegradation.
32097809	6	112	theme	CS	1483:1484	arg1	degradation					1486:1496	CS degradation	1483:1496	CS degradation	1483:1496	The relative abundances (RAs) of rumen bacteria were increased in the cosubstrate CS/WS and FW fermentation conditions, and the enhancement of CS degradation with FW supplementation was stronger than that of WS rumen fermentation with FW supplementation.
32961355	6	0	theme	histologic	846:855	arg1	analyses					857:864	histologic analyses	846:864	histologic analyses	846:864	Mucus thickness and quality were determined by histologic analyses.
32961355	11	1	theme	reduced	1527:1533	arg1	commensal					1546:1554	reduced protective commensal	1527:1554	reduced protective commensal	1527:1554	Feeding a HFrD also altered gut microbial populations and metabolism including reduced protective commensal and bile salt hydrolase-expressing microbes and increased luminal conjugated bile acids.
32961355	11	2	theme	hydrolase-expressing	1570:1589	arg1	microbes					1591:1598	hydrolase-expressing microbes	1570:1598	hydrolase-expressing microbes	1570:1598	Feeding a HFrD also altered gut microbial populations and metabolism including reduced protective commensal and bile salt hydrolase-expressing microbes and increased luminal conjugated bile acids.
32961355	1	3	theme	inflammatory	162:173	arg1	diseases					181:188	inflammatory bowel diseases	162:188	inflammatory bowel diseases	162:188	BACKGROUND & AIMS The incidence of inflammatory bowel diseases has increased over the last half century, suggesting a role for dietary factors.
32961355	8	4	theme	Citrobacter	1144:1154	arg1	rodentium					1156:1164	Citrobacter rodentium infection and Il10-/- mice	1144:1191	rodentium	1156:1164	The significance of HFrD on pathogen and genetic-driven models of colitis was determined by using Citrobacter rodentium infection and Il10-/- mice, respectively.
32961355	14	5	dep	CONCLUSIONS	1806:1816	arg1	has					1854:1856	has	1854:1856	has a pro-colitic effect that can be explained by changes in the composition, distribution, and metabolic function of resident enteric microbiota	1854:1998	CONCLUSIONS Excess dietary fructose consumption has a pro-colitic effect that can be explained by changes in the composition, distribution, and metabolic function of resident enteric microbiota.
32961355	11	6	theme	bile	1560:1563	arg1	salt					1565:1568	bile salt	1560:1568	bile salt	1560:1568	Feeding a HFrD also altered gut microbial populations and metabolism including reduced protective commensal and bile salt hydrolase-expressing microbes and increased luminal conjugated bile acids.
32961355	1	7	theme	diseases	181:188	arg1	incidence					149:157	The incidence	145:157	The incidence of inflammatory bowel diseases	145:188	BACKGROUND & AIMS The incidence of inflammatory bowel diseases has increased over the last half century, suggesting a role for dietary factors.
32961355	0	8	theme	Worsening	108:116	arg1	Colitis					118:124	Worsening Colitis	108:124	Worsening Colitis	108:124	Dietary Fructose Alters the Composition, Localization, and Metabolism of Gut Microbiota in Association With Worsening Colitis.
32961355	8	9	theme	Il10-/-	1180:1186	arg1	mice					1188:1191	Il10-/- mice	1180:1191	Il10-/- mice	1180:1191	The significance of HFrD on pathogen and genetic-driven models of colitis was determined by using Citrobacter rodentium infection and Il10-/- mice, respectively.
32961355	5	10	theme	colitis	790:796	arg1	worsening					777:785	HFrD-induced worsening	764:785	HFrD-induced worsening of colitis	764:796	METHODS Antibiotics and germ-free mice were used to determine the relevance of microbes for HFrD-induced worsening of colitis.
32961355	5	11	dep	METHODS	672:678	arg1	Antibiotics					680:690	Antibiotics	680:690	Antibiotics	680:690	METHODS Antibiotics and germ-free mice were used to determine the relevance of microbes for HFrD-induced worsening of colitis.
32961355	4	12	theme	genetic	645:651	arg1	models					653:658	both microbially driven and genetic models	617:658	both microbially driven and genetic models of colitis	617:669	The primary objectives of the current study were to elucidate the mechanism(s) underlying the pro-colitic effects of dietary fructose and to determine whether this effect occurs in both microbially driven and genetic models of colitis.
32961355	11	13	theme	microbial	1480:1488	arg1	commensal					1546:1554	reduced protective commensal	1527:1554	reduced protective commensal	1527:1554	Feeding a HFrD also altered gut microbial populations and metabolism including reduced protective commensal and bile salt hydrolase-expressing microbes and increased luminal conjugated bile acids.
32961355	11	13	theme	microbial	1480:1488	arg1	salt					1565:1568	bile salt	1560:1568	bile salt	1560:1568	Feeding a HFrD also altered gut microbial populations and metabolism including reduced protective commensal and bile salt hydrolase-expressing microbes and increased luminal conjugated bile acids.
32961355	11	13	theme	microbial	1480:1488	arg1	populations					1490:1500	gut microbial populations	1476:1500	gut microbial populations	1476:1500	Feeding a HFrD also altered gut microbial populations and metabolism including reduced protective commensal and bile salt hydrolase-expressing microbes and increased luminal conjugated bile acids.
32961355	7	14	theme	microbial	985:993	arg1	composition					995:1005	microbial composition	985:1005	microbial composition	985:1005	16S rRNA profiling, in situ hybridization, metatranscriptomic analyses, and fecal metabolomics were used to determine microbial composition, spatial distribution, and metabolism.
32961355	1	15	theme	BACKGROUND	127:136	arg1	&					138:138	BACKGROUND &	127:138	BACKGROUND &	127:138	BACKGROUND & AIMS The incidence of inflammatory bowel diseases has increased over the last half century, suggesting a role for dietary factors.
32961355	5	16	theme	germ-free	696:704	arg1	mice					706:709	germ-free mice	696:709	germ-free mice	696:709	METHODS Antibiotics and germ-free mice were used to determine the relevance of microbes for HFrD-induced worsening of colitis.
32961355	9	17	theme	HFrD-mediated	1260:1272	arg1	worsening					1274:1282	HFrD-mediated worsening	1260:1282	HFrD-mediated worsening of DSS-induced colitis	1260:1305	RESULTS Reducing or eliminating bacteria attenuated HFrD-mediated worsening of DSS-induced colitis.
32961355	4	18	theme	primary	440:446	arg1	objectives					448:457	The primary objectives	436:457	The primary objectives of the current study	436:478	The primary objectives of the current study were to elucidate the mechanism(s) underlying the pro-colitic effects of dietary fructose and to determine whether this effect occurs in both microbially driven and genetic models of colitis.
32961355	10	19	theme	mucus	1441:1445	arg1	quality					1422:1428	the quality	1418:1428	the quality of colonic mucus	1418:1445	HFrD feeding enhanced access of gut luminal microbes to the colonic mucosa by reducing thickness and altering the quality of colonic mucus.
32961355	4	20	theme	driven	634:639	arg1	models					653:658	both microbially driven and genetic models	617:658	both microbially driven and genetic models of colitis	617:669	The primary objectives of the current study were to elucidate the mechanism(s) underlying the pro-colitic effects of dietary fructose and to determine whether this effect occurs in both microbially driven and genetic models of colitis.
32961355	14	21	theme	pro-colitic	1860:1870	arg1	effect					1872:1877	a pro-colitic effect	1858:1877	a pro-colitic effect that can be explained by changes in the composition, distribution, and metabolic function of resident enteric microbiota	1858:1998	CONCLUSIONS Excess dietary fructose consumption has a pro-colitic effect that can be explained by changes in the composition, distribution, and metabolic function of resident enteric microbiota.
32961355	3	22	theme	high	335:338	arg1	HFrD					355:358	HFrD	355:358	HFrD	355:358	Recently, a high fructose diet (HFrD) was shown to enhance dextran sodium sulfate (DSS)-induced colitis in mice.
32961355	3	22	theme	high	335:338	arg1	diet					349:352	a high fructose diet	333:352	a high fructose diet (HFrD)	333:359	Recently, a high fructose diet (HFrD) was shown to enhance dextran sodium sulfate (DSS)-induced colitis in mice.
32961355	7	23	dep	in	887:888	arg1	situ					890:893	situ	890:893	situ	890:893	16S rRNA profiling, in situ hybridization, metatranscriptomic analyses, and fecal metabolomics were used to determine microbial composition, spatial distribution, and metabolism.
32961355	0	24	theme	Microbiota	77:86	arg1	Localization					41:52	Localization	41:52	Localization	41:52	Dietary Fructose Alters the Composition, Localization, and Metabolism of Gut Microbiota in Association With Worsening Colitis.
32961355	0	24	theme	Microbiota	77:86	arg1	Metabolism					59:68	Metabolism	59:68	Metabolism	59:68	Dietary Fructose Alters the Composition, Localization, and Metabolism of Gut Microbiota in Association With Worsening Colitis.
32961355	0	24	theme	Microbiota	77:86	arg1	Composition					28:38	Composition	28:38	Composition	28:38	Dietary Fructose Alters the Composition, Localization, and Metabolism of Gut Microbiota in Association With Worsening Colitis.
32961355	9	25	theme	colitis	1299:1305	arg1	worsening					1274:1282	HFrD-mediated worsening	1260:1282	HFrD-mediated worsening of DSS-induced colitis	1260:1305	RESULTS Reducing or eliminating bacteria attenuated HFrD-mediated worsening of DSS-induced colitis.
32961355	0	26	from	Localization	41:52	arg1	Association					91:101	Association	91:101	Association With Worsening Colitis	91:124	Dietary Fructose Alters the Composition, Localization, and Metabolism of Gut Microbiota in Association With Worsening Colitis.
32961355	12	27	theme	DSS-induced	1702:1712	arg1	colitis					1714:1720	DSS-induced colitis	1702:1720	DSS-induced colitis	1702:1720	Administration of conjugated bile acids to mice worsened DSS-induced colitis.
32961355	0	28	from	Composition	28:38	arg1	Association					91:101	Association	91:101	Association With Worsening Colitis	91:124	Dietary Fructose Alters the Composition, Localization, and Metabolism of Gut Microbiota in Association With Worsening Colitis.
32961355	0	29	theme	Dietary	0:6	arg1	Fructose					8:15	Dietary Fructose	0:15	Dietary Fructose	0:15	Dietary Fructose Alters the Composition, Localization, and Metabolism of Gut Microbiota in Association With Worsening Colitis.
32961355	1	30	theme	dietary	254:260	arg1	factors					262:268	dietary factors	254:268	dietary factors	254:268	BACKGROUND & AIMS The incidence of inflammatory bowel diseases has increased over the last half century, suggesting a role for dietary factors.
32961355	12	31	theme	conjugated	1663:1672	arg1	acids					1679:1683	conjugated bile acids	1663:1683	conjugated bile acids	1663:1683	Administration of conjugated bile acids to mice worsened DSS-induced colitis.
32961355	11	32	theme	protective	1535:1544	arg1	commensal					1546:1554	reduced protective commensal	1527:1554	reduced protective commensal	1527:1554	Feeding a HFrD also altered gut microbial populations and metabolism including reduced protective commensal and bile salt hydrolase-expressing microbes and increased luminal conjugated bile acids.
32961355	10	33	theme	HFrD	1308:1311	arg1	feeding					1313:1319	HFrD feeding	1308:1319	HFrD feeding	1308:1319	HFrD feeding enhanced access of gut luminal microbes to the colonic mucosa by reducing thickness and altering the quality of colonic mucus.
32961355	3	34	theme	-induced	410:417	arg1	colitis					419:425	dextran sodium sulfate (DSS)-induced colitis	382:425	dextran sodium sulfate (DSS)-induced colitis in mice	382:433	Recently, a high fructose diet (HFrD) was shown to enhance dextran sodium sulfate (DSS)-induced colitis in mice.
32961355	12	35	theme	acids	1679:1683	arg1	Administration					1645:1658	Administration	1645:1658	Administration of conjugated bile acids to mice	1645:1691	Administration of conjugated bile acids to mice worsened DSS-induced colitis.
32961355	4	36	theme	mechanism	502:510	arg1	s					512:512	the mechanism(s)	498:513	the mechanism(s) underlying the pro-colitic effects of dietary fructose	498:568	The primary objectives of the current study were to elucidate the mechanism(s) underlying the pro-colitic effects of dietary fructose and to determine whether this effect occurs in both microbially driven and genetic models of colitis.
32961355	11	37	theme	Feeding	1448:1454	arg1	HFrD					1458:1461	Feeding a HFrD	1448:1461	Feeding a HFrD	1448:1461	Feeding a HFrD also altered gut microbial populations and metabolism including reduced protective commensal and bile salt hydrolase-expressing microbes and increased luminal conjugated bile acids.
32961355	14	38	theme	dietary	1825:1831	arg1	consumption					1842:1852	Excess dietary fructose consumption	1818:1852	Excess dietary fructose consumption	1818:1852	CONCLUSIONS Excess dietary fructose consumption has a pro-colitic effect that can be explained by changes in the composition, distribution, and metabolic function of resident enteric microbiota.
32961355	5	39	used	used	716:719	arg2	mice					706:709	germ-free mice	696:709	germ-free mice	696:709	METHODS Antibiotics and germ-free mice were used to determine the relevance of microbes for HFrD-induced worsening of colitis.
32961355	5	39	used	used	716:719	arg2	METHODS					672:678	METHODS	672:678	METHODS	672:678	METHODS Antibiotics and germ-free mice were used to determine the relevance of microbes for HFrD-induced worsening of colitis.
32961355	6	40	theme	Mucus	799:803	arg1	thickness					805:813	Mucus thickness	799:813	Mucus thickness	799:813	Mucus thickness and quality were determined by histologic analyses.
32961355	4	41	theme	current	466:472	arg1	study					474:478	the current study	462:478	the current study	462:478	The primary objectives of the current study were to elucidate the mechanism(s) underlying the pro-colitic effects of dietary fructose and to determine whether this effect occurs in both microbially driven and genetic models of colitis.
32961355	11	42	theme	conjugated	1622:1631	arg1	acids					1638:1642	luminal conjugated bile acids	1614:1642	luminal conjugated bile acids	1614:1642	Feeding a HFrD also altered gut microbial populations and metabolism including reduced protective commensal and bile salt hydrolase-expressing microbes and increased luminal conjugated bile acids.
32961355	0	43	dep	Composition	28:38	arg1	the					24:26	the	24:26	the	24:26	Dietary Fructose Alters the Composition, Localization, and Metabolism of Gut Microbiota in Association With Worsening Colitis.
32961355	3	44	from	colitis	419:425	arg1	mice					430:433	mice	430:433	mice	430:433	Recently, a high fructose diet (HFrD) was shown to enhance dextran sodium sulfate (DSS)-induced colitis in mice.
32961355	14	45	contain	has	1854:1856	arg1	consumption					1842:1852	Excess dietary fructose consumption	1818:1852	Excess dietary fructose consumption	1818:1852	CONCLUSIONS Excess dietary fructose consumption has a pro-colitic effect that can be explained by changes in the composition, distribution, and metabolic function of resident enteric microbiota.
32961355	14	45	contain	has	1854:1856	arg2	effect					1872:1877	a pro-colitic effect	1858:1877	a pro-colitic effect that can be explained by changes in the composition, distribution, and metabolic function of resident enteric microbiota	1858:1998	CONCLUSIONS Excess dietary fructose consumption has a pro-colitic effect that can be explained by changes in the composition, distribution, and metabolic function of resident enteric microbiota.
32961355	7	46	theme	16S	867:869	arg1	rRNA					871:874	16S rRNA profiling	867:884	16S rRNA profiling	867:884	16S rRNA profiling, in situ hybridization, metatranscriptomic analyses, and fecal metabolomics were used to determine microbial composition, spatial distribution, and metabolism.
32961355	1	47	theme	last	213:216	arg1	century					223:229	the last half century	209:229	the last half century	209:229	BACKGROUND & AIMS The incidence of inflammatory bowel diseases has increased over the last half century, suggesting a role for dietary factors.
32961355	11	48	theme	luminal	1614:1620	arg1	acids					1638:1642	luminal conjugated bile acids	1614:1642	luminal conjugated bile acids	1614:1642	Feeding a HFrD also altered gut microbial populations and metabolism including reduced protective commensal and bile salt hydrolase-expressing microbes and increased luminal conjugated bile acids.
32961355	11	49	dep	commensal	1546:1554	arg1	microbes					1591:1598	hydrolase-expressing microbes	1570:1598	hydrolase-expressing microbes	1570:1598	Feeding a HFrD also altered gut microbial populations and metabolism including reduced protective commensal and bile salt hydrolase-expressing microbes and increased luminal conjugated bile acids.
32961355	4	50	theme	pro-colitic	530:540	arg1	effects					542:548	the pro-colitic effects	526:548	the pro-colitic effects of dietary fructose	526:568	The primary objectives of the current study were to elucidate the mechanism(s) underlying the pro-colitic effects of dietary fructose and to determine whether this effect occurs in both microbially driven and genetic models of colitis.
32961355	10	51	theme	luminal	1344:1350	arg1	microbes					1352:1359	gut luminal microbes	1340:1359	gut luminal microbes	1340:1359	HFrD feeding enhanced access of gut luminal microbes to the colonic mucosa by reducing thickness and altering the quality of colonic mucus.
32961355	2	52	theme	Fructose	271:278	arg1	consumption					280:290	Fructose consumption	271:290	Fructose consumption	271:290	Fructose consumption has increased in recent years.
32961355	8	53	theme	colitis	1112:1118	arg1	models					1102:1107	pathogen and genetic-driven models	1074:1107	models	1102:1107	The significance of HFrD on pathogen and genetic-driven models of colitis was determined by using Citrobacter rodentium infection and Il10-/- mice, respectively.
32961355	10	54	theme	colonic	1368:1374	arg1	mucosa					1376:1381	the colonic mucosa	1364:1381	the colonic mucosa	1364:1381	HFrD feeding enhanced access of gut luminal microbes to the colonic mucosa by reducing thickness and altering the quality of colonic mucus.
32961355	14	55	theme	metabolic	1950:1958	arg1	function					1960:1967	metabolic function	1950:1967	metabolic function	1950:1967	CONCLUSIONS Excess dietary fructose consumption has a pro-colitic effect that can be explained by changes in the composition, distribution, and metabolic function of resident enteric microbiota.
32961355	0	56	with	Association	91:101	arg1	Colitis					118:124	Worsening Colitis	108:124	Worsening Colitis	108:124	Dietary Fructose Alters the Composition, Localization, and Metabolism of Gut Microbiota in Association With Worsening Colitis.
32961355	1	57	theme	bowel	175:179	arg1	diseases					181:188	inflammatory bowel diseases	162:188	inflammatory bowel diseases	162:188	BACKGROUND & AIMS The incidence of inflammatory bowel diseases has increased over the last half century, suggesting a role for dietary factors.
32961355	13	58	theme	C	1793:1793	arg1	rodentium					1795:1803	C rodentium	1793:1803	C rodentium	1793:1803	The HFrD also worsened colitis in Il10-/- mice and mice infected with C rodentium.
32961355	14	59	theme	microbiota	1989:1998	arg1	composition					1919:1929	composition	1919:1929	composition	1919:1929	CONCLUSIONS Excess dietary fructose consumption has a pro-colitic effect that can be explained by changes in the composition, distribution, and metabolic function of resident enteric microbiota.
32961355	14	59	theme	microbiota	1989:1998	arg1	function					1960:1967	metabolic function	1950:1967	metabolic function	1950:1967	CONCLUSIONS Excess dietary fructose consumption has a pro-colitic effect that can be explained by changes in the composition, distribution, and metabolic function of resident enteric microbiota.
32961355	14	59	theme	microbiota	1989:1998	arg1	distribution					1932:1943	distribution	1932:1943	distribution	1932:1943	CONCLUSIONS Excess dietary fructose consumption has a pro-colitic effect that can be explained by changes in the composition, distribution, and metabolic function of resident enteric microbiota.
32961355	5	60	theme	HFrD-induced	764:775	arg1	worsening					777:785	HFrD-induced worsening	764:785	HFrD-induced worsening of colitis	764:796	METHODS Antibiotics and germ-free mice were used to determine the relevance of microbes for HFrD-induced worsening of colitis.
32961355	4	61	theme	fructose	561:568	arg1	effects					542:548	the pro-colitic effects	526:548	the pro-colitic effects of dietary fructose	526:568	The primary objectives of the current study were to elucidate the mechanism(s) underlying the pro-colitic effects of dietary fructose and to determine whether this effect occurs in both microbially driven and genetic models of colitis.
32961355	13	62	theme	Il10-/-	1757:1763	arg1	mice					1765:1768	mice	1765:1768	mice	1765:1768	The HFrD also worsened colitis in Il10-/- mice and mice infected with C rodentium.
32961355	7	63	theme	spatial	1008:1014	arg1	distribution					1016:1027	spatial distribution	1008:1027	spatial distribution	1008:1027	16S rRNA profiling, in situ hybridization, metatranscriptomic analyses, and fecal metabolomics were used to determine microbial composition, spatial distribution, and metabolism.
32961355	0	64	from	Metabolism	59:68	arg1	Association					91:101	Association	91:101	Association With Worsening Colitis	91:124	Dietary Fructose Alters the Composition, Localization, and Metabolism of Gut Microbiota in Association With Worsening Colitis.
32961355	11	65	theme	gut	1476:1478	arg1	commensal					1546:1554	reduced protective commensal	1527:1554	reduced protective commensal	1527:1554	Feeding a HFrD also altered gut microbial populations and metabolism including reduced protective commensal and bile salt hydrolase-expressing microbes and increased luminal conjugated bile acids.
32961355	11	65	theme	gut	1476:1478	arg1	salt					1565:1568	bile salt	1560:1568	bile salt	1560:1568	Feeding a HFrD also altered gut microbial populations and metabolism including reduced protective commensal and bile salt hydrolase-expressing microbes and increased luminal conjugated bile acids.
32961355	11	65	theme	gut	1476:1478	arg1	populations					1490:1500	gut microbial populations	1476:1500	gut microbial populations	1476:1500	Feeding a HFrD also altered gut microbial populations and metabolism including reduced protective commensal and bile salt hydrolase-expressing microbes and increased luminal conjugated bile acids.
32961355	9	66	theme	eliminating	1228:1238	arg1	bacteria					1240:1247	eliminating bacteria	1228:1247	eliminating bacteria	1228:1247	RESULTS Reducing or eliminating bacteria attenuated HFrD-mediated worsening of DSS-induced colitis.
32961355	8	67	from	significance	1050:1061	arg1	models					1102:1107	pathogen and genetic-driven models	1074:1107	models	1102:1107	The significance of HFrD on pathogen and genetic-driven models of colitis was determined by using Citrobacter rodentium infection and Il10-/- mice, respectively.
32961355	7	68	theme	fecal	943:947	arg1	metabolomics					949:960	fecal metabolomics	943:960	fecal metabolomics	943:960	16S rRNA profiling, in situ hybridization, metatranscriptomic analyses, and fecal metabolomics were used to determine microbial composition, spatial distribution, and metabolism.
32961355	7	69	used	used	967:970	arg2	rRNA					871:874	16S rRNA profiling	867:884	16S rRNA profiling	867:884	16S rRNA profiling, in situ hybridization, metatranscriptomic analyses, and fecal metabolomics were used to determine microbial composition, spatial distribution, and metabolism.
32961355	7	69	used	used	967:970	arg2	hybridization					895:907	in situ hybridization	887:907	in situ hybridization	887:907	16S rRNA profiling, in situ hybridization, metatranscriptomic analyses, and fecal metabolomics were used to determine microbial composition, spatial distribution, and metabolism.
32961355	7	69	used	used	967:970	arg2	metabolomics					949:960	fecal metabolomics	943:960	fecal metabolomics	943:960	16S rRNA profiling, in situ hybridization, metatranscriptomic analyses, and fecal metabolomics were used to determine microbial composition, spatial distribution, and metabolism.
32961355	7	69	used	used	967:970	arg2	analyses					929:936	metatranscriptomic analyses	910:936	metatranscriptomic analyses	910:936	16S rRNA profiling, in situ hybridization, metatranscriptomic analyses, and fecal metabolomics were used to determine microbial composition, spatial distribution, and metabolism.
32961355	0	70	theme	Gut	73:75	arg1	Microbiota					77:86	Gut Microbiota	73:86	Gut Microbiota	73:86	Dietary Fructose Alters the Composition, Localization, and Metabolism of Gut Microbiota in Association With Worsening Colitis.
32961355	10	71	theme	colonic	1433:1439	arg1	mucus					1441:1445	colonic mucus	1433:1445	colonic mucus	1433:1445	HFrD feeding enhanced access of gut luminal microbes to the colonic mucosa by reducing thickness and altering the quality of colonic mucus.
32961355	9	72	theme	DSS-induced	1287:1297	arg1	colitis					1299:1305	DSS-induced colitis	1287:1305	DSS-induced colitis	1287:1305	RESULTS Reducing or eliminating bacteria attenuated HFrD-mediated worsening of DSS-induced colitis.
32961355	7	73	theme	metatranscriptomic	910:927	arg1	analyses					929:936	metatranscriptomic analyses	910:936	metatranscriptomic analyses	910:936	16S rRNA profiling, in situ hybridization, metatranscriptomic analyses, and fecal metabolomics were used to determine microbial composition, spatial distribution, and metabolism.
32961355	3	74	theme	fructose	340:347	arg1	HFrD					355:358	HFrD	355:358	HFrD	355:358	Recently, a high fructose diet (HFrD) was shown to enhance dextran sodium sulfate (DSS)-induced colitis in mice.
32961355	3	74	theme	fructose	340:347	arg1	diet					349:352	a high fructose diet	333:352	a high fructose diet (HFrD)	333:359	Recently, a high fructose diet (HFrD) was shown to enhance dextran sodium sulfate (DSS)-induced colitis in mice.
32961355	7	75	theme	in	887:888	arg1	hybridization					895:907	in situ hybridization	887:907	in situ hybridization	887:907	16S rRNA profiling, in situ hybridization, metatranscriptomic analyses, and fecal metabolomics were used to determine microbial composition, spatial distribution, and metabolism.
32961355	4	76	theme	colitis	663:669	arg1	models					653:658	both microbially driven and genetic models	617:658	both microbially driven and genetic models of colitis	617:669	The primary objectives of the current study were to elucidate the mechanism(s) underlying the pro-colitic effects of dietary fructose and to determine whether this effect occurs in both microbially driven and genetic models of colitis.
32961355	11	77	theme	a	1456:1456	arg1	HFrD					1458:1461	Feeding a HFrD	1448:1461	Feeding a HFrD	1448:1461	Feeding a HFrD also altered gut microbial populations and metabolism including reduced protective commensal and bile salt hydrolase-expressing microbes and increased luminal conjugated bile acids.
32961355	5	78	theme	microbes	751:758	arg1	relevance					738:746	the relevance	734:746	the relevance of microbes for HFrD-induced worsening of colitis	734:796	METHODS Antibiotics and germ-free mice were used to determine the relevance of microbes for HFrD-induced worsening of colitis.
32961355	7	79	theme	profiling	876:884	arg1	rRNA					871:874	16S rRNA profiling	867:884	16S rRNA profiling	867:884	16S rRNA profiling, in situ hybridization, metatranscriptomic analyses, and fecal metabolomics were used to determine microbial composition, spatial distribution, and metabolism.
32961355	14	80	theme	fructose	1833:1840	arg1	consumption					1842:1852	Excess dietary fructose consumption	1818:1852	Excess dietary fructose consumption	1818:1852	CONCLUSIONS Excess dietary fructose consumption has a pro-colitic effect that can be explained by changes in the composition, distribution, and metabolic function of resident enteric microbiota.
32961355	12	81	theme	bile	1674:1677	arg1	acids					1679:1683	conjugated bile acids	1663:1683	conjugated bile acids	1663:1683	Administration of conjugated bile acids to mice worsened DSS-induced colitis.
32961355	4	82	theme	study	474:478	arg1	objectives					448:457	The primary objectives	436:457	The primary objectives of the current study	436:478	The primary objectives of the current study were to elucidate the mechanism(s) underlying the pro-colitic effects of dietary fructose and to determine whether this effect occurs in both microbially driven and genetic models of colitis.
32961355	10	83	theme	gut	1340:1342	arg1	microbes					1352:1359	gut luminal microbes	1340:1359	gut luminal microbes	1340:1359	HFrD feeding enhanced access of gut luminal microbes to the colonic mucosa by reducing thickness and altering the quality of colonic mucus.
32961355	14	84	theme	Excess	1818:1823	arg1	consumption					1842:1852	Excess dietary fructose consumption	1818:1852	Excess dietary fructose consumption	1818:1852	CONCLUSIONS Excess dietary fructose consumption has a pro-colitic effect that can be explained by changes in the composition, distribution, and metabolic function of resident enteric microbiota.
32961355	8	85	theme	HFrD	1066:1069	arg1	significance					1050:1061	The significance	1046:1061	The significance of HFrD on pathogen and genetic-driven models of colitis	1046:1118	The significance of HFrD on pathogen and genetic-driven models of colitis was determined by using Citrobacter rodentium infection and Il10-/- mice, respectively.
32961355	8	86	dep	rodentium	1156:1164	arg1	infection					1166:1174	infection	1166:1174	infection	1166:1174	The significance of HFrD on pathogen and genetic-driven models of colitis was determined by using Citrobacter rodentium infection and Il10-/- mice, respectively.
32961355	14	87	theme	resident	1972:1979	arg1	microbiota					1989:1998	resident enteric microbiota	1972:1998	resident enteric microbiota	1972:1998	CONCLUSIONS Excess dietary fructose consumption has a pro-colitic effect that can be explained by changes in the composition, distribution, and metabolic function of resident enteric microbiota.
32961355	8	88	theme	pathogen	1074:1081	arg1	models					1102:1107	pathogen and genetic-driven models	1074:1107	models	1102:1107	The significance of HFrD on pathogen and genetic-driven models of colitis was determined by using Citrobacter rodentium infection and Il10-/- mice, respectively.
32961355	14	89	theme	enteric	1981:1987	arg1	microbiota					1989:1998	resident enteric microbiota	1972:1998	resident enteric microbiota	1972:1998	CONCLUSIONS Excess dietary fructose consumption has a pro-colitic effect that can be explained by changes in the composition, distribution, and metabolic function of resident enteric microbiota.
32961355	10	90	theme	microbes	1352:1359	arg1	access					1330:1335	access	1330:1335	access of gut luminal microbes to the colonic mucosa	1330:1381	HFrD feeding enhanced access of gut luminal microbes to the colonic mucosa by reducing thickness and altering the quality of colonic mucus.
32961355	9	91	dep	RESULTS	1208:1214	arg1	Reducing					1216:1223	Reducing	1216:1223	Reducing	1216:1223	RESULTS Reducing or eliminating bacteria attenuated HFrD-mediated worsening of DSS-induced colitis.
32961355	9	91	dep	RESULTS	1208:1214	arg1	bacteria					1240:1247	eliminating bacteria	1228:1247	eliminating bacteria	1228:1247	RESULTS Reducing or eliminating bacteria attenuated HFrD-mediated worsening of DSS-induced colitis.
32961355	2	92	theme	recent	309:314	arg1	years					316:320	recent years	309:320	recent years	309:320	Fructose consumption has increased in recent years.
32961355	14	93	from	changes	1904:1910	arg1	composition					1919:1929	composition	1919:1929	composition	1919:1929	CONCLUSIONS Excess dietary fructose consumption has a pro-colitic effect that can be explained by changes in the composition, distribution, and metabolic function of resident enteric microbiota.
32961355	14	93	from	changes	1904:1910	arg1	function					1960:1967	metabolic function	1950:1967	metabolic function	1950:1967	CONCLUSIONS Excess dietary fructose consumption has a pro-colitic effect that can be explained by changes in the composition, distribution, and metabolic function of resident enteric microbiota.
32961355	14	93	from	changes	1904:1910	arg1	distribution					1932:1943	distribution	1932:1943	distribution	1932:1943	CONCLUSIONS Excess dietary fructose consumption has a pro-colitic effect that can be explained by changes in the composition, distribution, and metabolic function of resident enteric microbiota.
32961355	1	94	theme	half	218:221	arg1	century					223:229	the last half century	209:229	the last half century	209:229	BACKGROUND & AIMS The incidence of inflammatory bowel diseases has increased over the last half century, suggesting a role for dietary factors.
32961355	8	95	theme	genetic-driven	1087:1100	arg1	models					1102:1107	pathogen and genetic-driven models	1074:1107	models	1102:1107	The significance of HFrD on pathogen and genetic-driven models of colitis was determined by using Citrobacter rodentium infection and Il10-/- mice, respectively.
32961355	4	96	theme	dietary	553:559	arg1	fructose					561:568	dietary fructose	553:568	dietary fructose	553:568	The primary objectives of the current study were to elucidate the mechanism(s) underlying the pro-colitic effects of dietary fructose and to determine whether this effect occurs in both microbially driven and genetic models of colitis.
32961355	11	97	theme	bile	1633:1636	arg1	acids					1638:1642	luminal conjugated bile acids	1614:1642	luminal conjugated bile acids	1614:1642	Feeding a HFrD also altered gut microbial populations and metabolism including reduced protective commensal and bile salt hydrolase-expressing microbes and increased luminal conjugated bile acids.
31944159	0	0	theme	post-operative	81:94	arg1	enterocolitis					96:108	post-operative enterocolitis	81:108	post-operative enterocolitis	81:108	Prospective study reveals a microbiome signature that predicts the occurrence of post-operative enterocolitis in Hirschsprung disease (HSCR) patients.
31944159	4	1	contain	containing	663:672	arg1	signature					653:661	a microbiome signature	640:661	a microbiome signature containing 21 operational taxonomic units (OTUs) that can potentially predict postoperative HAEC with ~85% accuracy	640:777	We identified a microbiome signature containing 21 operational taxonomic units (OTUs) that can potentially predict postoperative HAEC with ~85% accuracy.
31944159	4	1	contain	containing	663:672	arg2	units					699:703	21 operational taxonomic units	674:703	21 operational taxonomic units (OTUs) that can potentially predict postoperative HAEC with ~85% accuracy	674:777	We identified a microbiome signature containing 21 operational taxonomic units (OTUs) that can potentially predict postoperative HAEC with ~85% accuracy.
31944159	4	1	contain	containing	663:672	arg2	OTUs					706:709	OTUs	706:709	OTUs	706:709	We identified a microbiome signature containing 21 operational taxonomic units (OTUs) that can potentially predict postoperative HAEC with ~85% accuracy.
31944159	5	2	theme	total	864:868	arg1	HAEC					870:873	total HAEC	864:873	total HAEC (i.e., preoperative and postoperative HAEC combined)	864:926	Furthermore, we identified exclusive breastfeeding as a novel protective factor for total HAEC (i.e., preoperative and postoperative HAEC combined).
31944159	1	3	theme	live	238:241	arg1	births					243:248	1/5,000 live births	230:248	1/5,000 live births	230:248	Hirschsprung disease (HSCR) is a birth defect with an approximate incidence of 1/5,000 live births, and up to one-third of HSCR patients develop Hirschsprung-associated enterocolitis (HAEC), the leading cause of HSCR-related death.
31944159	1	4	theme	births	243:248	arg1	incidence					217:225	an approximate incidence	202:225	an approximate incidence of 1/5,000 live births	202:248	Hirschsprung disease (HSCR) is a birth defect with an approximate incidence of 1/5,000 live births, and up to one-third of HSCR patients develop Hirschsprung-associated enterocolitis (HAEC), the leading cause of HSCR-related death.
31944159	6	5	theme	LPS	1156:1158	arg1	concentrations					1160:1173	lower LPS concentrations	1150:1173	lower LPS concentrations	1150:1173	In addition, we discovered that breastfeeding was associated with a lowered risk for HAEC potentially mediated by modulating the gut microbiome composition characterized by a lower abundance of Gram-negative bacteria and lower LPS concentrations.
31944159	0	6	theme	enterocolitis	96:108	arg1	occurrence					67:76	the occurrence	63:76	the occurrence of post-operative enterocolitis in Hirschsprung disease (HSCR) patients	63:148	Prospective study reveals a microbiome signature that predicts the occurrence of post-operative enterocolitis in Hirschsprung disease (HSCR) patients.
31944159	6	7	theme	lower	1150:1154	arg1	concentrations					1160:1173	lower LPS concentrations	1150:1173	lower LPS concentrations	1150:1173	In addition, we discovered that breastfeeding was associated with a lowered risk for HAEC potentially mediated by modulating the gut microbiome composition characterized by a lower abundance of Gram-negative bacteria and lower LPS concentrations.
31944159	4	8	with	HAEC	755:758	arg1	accuracy					770:777	~85% accuracy	765:777	~85% accuracy	765:777	We identified a microbiome signature containing 21 operational taxonomic units (OTUs) that can potentially predict postoperative HAEC with ~85% accuracy.
31944159	0	9	theme	disease	126:132	arg1	patients					141:148	Hirschsprung disease (HSCR) patients	113:148	Hirschsprung disease (HSCR) patients	113:148	Prospective study reveals a microbiome signature that predicts the occurrence of post-operative enterocolitis in Hirschsprung disease (HSCR) patients.
31944159	7	10	theme	HAEC	1264:1267	arg1	progression					1269:1279	HAEC progression	1264:1279	HAEC progression	1264:1279	In conclusion, modulating the gut microbiome by encouraging breastfeeding might prevent HAEC progression in HSCR patients.
31944159	4	11	theme	postoperative	741:753	arg1	HAEC					755:758	postoperative HAEC	741:758	postoperative HAEC with ~85% accuracy	741:777	We identified a microbiome signature containing 21 operational taxonomic units (OTUs) that can potentially predict postoperative HAEC with ~85% accuracy.
31944159	0	12	theme	Hirschsprung	113:124	arg1	HSCR					135:138	HSCR	135:138	HSCR	135:138	Prospective study reveals a microbiome signature that predicts the occurrence of post-operative enterocolitis in Hirschsprung disease (HSCR) patients.
31944159	0	12	theme	Hirschsprung	113:124	arg1	disease					126:132	Hirschsprung disease	113:132	Hirschsprung disease (HSCR) patients	113:148	Prospective study reveals a microbiome signature that predicts the occurrence of post-operative enterocolitis in Hirschsprung disease (HSCR) patients.
31944159	1	13	theme	leading	346:352	arg1	enterocolitis					320:332	Hirschsprung-associated enterocolitis	296:332	Hirschsprung-associated enterocolitis (HAEC)	296:339	Hirschsprung disease (HSCR) is a birth defect with an approximate incidence of 1/5,000 live births, and up to one-third of HSCR patients develop Hirschsprung-associated enterocolitis (HAEC), the leading cause of HSCR-related death.
31944159	1	13	theme	leading	346:352	arg1	cause					354:358	the leading cause	342:358	the leading cause of HSCR-related death	342:380	Hirschsprung disease (HSCR) is a birth defect with an approximate incidence of 1/5,000 live births, and up to one-third of HSCR patients develop Hirschsprung-associated enterocolitis (HAEC), the leading cause of HSCR-related death.
31944159	3	14	theme	prospective	485:495	arg1	study					497:501	a prospective study	483:501	a prospective study to investigate the enteric microbiome composition at the time of surgery as a predictor for developing postoperative HAEC	483:623	Here, we used a prospective study to investigate the enteric microbiome composition at the time of surgery as a predictor for developing postoperative HAEC.
31944159	6	15	theme	Gram-negative	1123:1135	arg1	bacteria					1137:1144	Gram-negative bacteria	1123:1144	Gram-negative bacteria	1123:1144	In addition, we discovered that breastfeeding was associated with a lowered risk for HAEC potentially mediated by modulating the gut microbiome composition characterized by a lower abundance of Gram-negative bacteria and lower LPS concentrations.
31944159	3	16	theme	enteric	522:528	arg1	composition					541:551	the enteric microbiome composition	518:551	the enteric microbiome composition	518:551	Here, we used a prospective study to investigate the enteric microbiome composition at the time of surgery as a predictor for developing postoperative HAEC.
31944159	3	16	theme	enteric	522:528	arg1	predictor					581:589	a predictor	579:589	a predictor for developing postoperative HAEC	579:623	Here, we used a prospective study to investigate the enteric microbiome composition at the time of surgery as a predictor for developing postoperative HAEC.
31944159	5	17	theme	novel	836:840	arg1	factor					853:858	a novel protective factor	834:858	a novel protective factor for total HAEC (i.e., preoperative and postoperative HAEC combined)	834:926	Furthermore, we identified exclusive breastfeeding as a novel protective factor for total HAEC (i.e., preoperative and postoperative HAEC combined).
31944159	5	17	theme	novel	836:840	arg1	breastfeeding					817:829	exclusive breastfeeding	807:829	exclusive breastfeeding	807:829	Furthermore, we identified exclusive breastfeeding as a novel protective factor for total HAEC (i.e., preoperative and postoperative HAEC combined).
31944159	0	18	theme	Prospective	0:10	arg1	study					12:16	Prospective study	0:16	Prospective study	0:16	Prospective study reveals a microbiome signature that predicts the occurrence of post-operative enterocolitis in Hirschsprung disease (HSCR) patients.
31944159	2	19	dep	pathogenesis	414:425	arg1	the					410:412	the	410:412	the	410:412	Very little is known about the pathogenesis, prevention, and early diagnosis of HAEC.
31944159	3	20	theme	microbiome	530:539	arg1	composition					541:551	the enteric microbiome composition	518:551	the enteric microbiome composition	518:551	Here, we used a prospective study to investigate the enteric microbiome composition at the time of surgery as a predictor for developing postoperative HAEC.
31944159	3	20	theme	microbiome	530:539	arg1	predictor					581:589	a predictor	579:589	a predictor for developing postoperative HAEC	579:623	Here, we used a prospective study to investigate the enteric microbiome composition at the time of surgery as a predictor for developing postoperative HAEC.
31944159	3	21	theme	postoperative	606:618	arg1	HAEC					620:623	postoperative HAEC	606:623	postoperative HAEC	606:623	Here, we used a prospective study to investigate the enteric microbiome composition at the time of surgery as a predictor for developing postoperative HAEC.
31944159	4	22	theme	%	768:768	arg1	accuracy					770:777	~85% accuracy	765:777	~85% accuracy	765:777	We identified a microbiome signature containing 21 operational taxonomic units (OTUs) that can potentially predict postoperative HAEC with ~85% accuracy.
31944159	5	23	theme	preoperative	882:893	arg1	HAEC					913:916	preoperative and postoperative HAEC	882:916	preoperative and postoperative HAEC combined	882:925	Furthermore, we identified exclusive breastfeeding as a novel protective factor for total HAEC (i.e., preoperative and postoperative HAEC combined).
31944159	6	24	theme	lowered	997:1003	arg1	risk					1005:1008	a lowered risk	995:1008	a lowered risk for HAEC potentially mediated by modulating the gut microbiome composition characterized by a lower abundance of Gram-negative bacteria and lower LPS concentrations	995:1173	In addition, we discovered that breastfeeding was associated with a lowered risk for HAEC potentially mediated by modulating the gut microbiome composition characterized by a lower abundance of Gram-negative bacteria and lower LPS concentrations.
31944159	1	25	theme	birth	184:188	arg1	defect					190:195	a birth defect	182:195	a birth defect with an approximate incidence of 1/5,000 live births	182:248	Hirschsprung disease (HSCR) is a birth defect with an approximate incidence of 1/5,000 live births, and up to one-third of HSCR patients develop Hirschsprung-associated enterocolitis (HAEC), the leading cause of HSCR-related death.
31944159	1	25	theme	birth	184:188	arg1	disease					164:170	Hirschsprung disease	151:170	Hirschsprung disease (HSCR)	151:177	Hirschsprung disease (HSCR) is a birth defect with an approximate incidence of 1/5,000 live births, and up to one-third of HSCR patients develop Hirschsprung-associated enterocolitis (HAEC), the leading cause of HSCR-related death.
31944159	4	26	theme	~85	765:767	arg1	%					768:768	%	768:768	%	768:768	We identified a microbiome signature containing 21 operational taxonomic units (OTUs) that can potentially predict postoperative HAEC with ~85% accuracy.
31944159	5	27	theme	postoperative	899:911	arg1	HAEC					913:916	preoperative and postoperative HAEC	882:916	preoperative and postoperative HAEC combined	882:925	Furthermore, we identified exclusive breastfeeding as a novel protective factor for total HAEC (i.e., preoperative and postoperative HAEC combined).
31944159	6	28	theme	lower	1104:1108	arg1	abundance					1110:1118	a lower abundance	1102:1118	a lower abundance of Gram-negative bacteria	1102:1144	In addition, we discovered that breastfeeding was associated with a lowered risk for HAEC potentially mediated by modulating the gut microbiome composition characterized by a lower abundance of Gram-negative bacteria and lower LPS concentrations.
31944159	5	29	dep	HAEC	913:916	arg1	i.e.					876:879	i.e.	876:879	i.e.	876:879	Furthermore, we identified exclusive breastfeeding as a novel protective factor for total HAEC (i.e., preoperative and postoperative HAEC combined).
31944159	2	30	theme	early	444:448	arg1	diagnosis					450:458	early diagnosis	444:458	early diagnosis	444:458	Very little is known about the pathogenesis, prevention, and early diagnosis of HAEC.
31944159	6	31	theme	microbiome	1062:1071	arg1	composition					1073:1083	the gut microbiome composition	1054:1083	the gut microbiome composition characterized by a lower abundance of Gram-negative bacteria and lower LPS concentrations	1054:1173	In addition, we discovered that breastfeeding was associated with a lowered risk for HAEC potentially mediated by modulating the gut microbiome composition characterized by a lower abundance of Gram-negative bacteria and lower LPS concentrations.
31944159	1	32	theme	HSCR-related	363:374	arg1	death					376:380	HSCR-related death	363:380	HSCR-related death	363:380	Hirschsprung disease (HSCR) is a birth defect with an approximate incidence of 1/5,000 live births, and up to one-third of HSCR patients develop Hirschsprung-associated enterocolitis (HAEC), the leading cause of HSCR-related death.
31944159	5	33	theme	protective	842:851	arg1	factor					853:858	a novel protective factor	834:858	a novel protective factor for total HAEC (i.e., preoperative and postoperative HAEC combined)	834:926	Furthermore, we identified exclusive breastfeeding as a novel protective factor for total HAEC (i.e., preoperative and postoperative HAEC combined).
31944159	5	33	theme	protective	842:851	arg1	breastfeeding					817:829	exclusive breastfeeding	807:829	exclusive breastfeeding	807:829	Furthermore, we identified exclusive breastfeeding as a novel protective factor for total HAEC (i.e., preoperative and postoperative HAEC combined).
31944159	5	34	theme	exclusive	807:815	arg1	factor					853:858	a novel protective factor	834:858	a novel protective factor for total HAEC (i.e., preoperative and postoperative HAEC combined)	834:926	Furthermore, we identified exclusive breastfeeding as a novel protective factor for total HAEC (i.e., preoperative and postoperative HAEC combined).
31944159	5	34	theme	exclusive	807:815	arg1	breastfeeding					817:829	exclusive breastfeeding	807:829	exclusive breastfeeding	807:829	Furthermore, we identified exclusive breastfeeding as a novel protective factor for total HAEC (i.e., preoperative and postoperative HAEC combined).
31944159	0	35	theme	microbiome	28:37	arg1	signature					39:47	a microbiome signature	26:47	a microbiome signature that predicts the occurrence of post-operative enterocolitis in Hirschsprung disease (HSCR) patients	26:148	Prospective study reveals a microbiome signature that predicts the occurrence of post-operative enterocolitis in Hirschsprung disease (HSCR) patients.
31944159	1	36	theme	HSCR	274:277	arg1	patients					279:286	HSCR patients	274:286	HSCR patients	274:286	Hirschsprung disease (HSCR) is a birth defect with an approximate incidence of 1/5,000 live births, and up to one-third of HSCR patients develop Hirschsprung-associated enterocolitis (HAEC), the leading cause of HSCR-related death.
31944159	1	37	with	defect	190:195	arg1	incidence					217:225	an approximate incidence	202:225	an approximate incidence of 1/5,000 live births	202:248	Hirschsprung disease (HSCR) is a birth defect with an approximate incidence of 1/5,000 live births, and up to one-third of HSCR patients develop Hirschsprung-associated enterocolitis (HAEC), the leading cause of HSCR-related death.
31944159	1	38	theme	death	376:380	arg1	enterocolitis					320:332	Hirschsprung-associated enterocolitis	296:332	Hirschsprung-associated enterocolitis (HAEC)	296:339	Hirschsprung disease (HSCR) is a birth defect with an approximate incidence of 1/5,000 live births, and up to one-third of HSCR patients develop Hirschsprung-associated enterocolitis (HAEC), the leading cause of HSCR-related death.
31944159	1	38	theme	death	376:380	arg1	cause					354:358	the leading cause	342:358	the leading cause of HSCR-related death	342:380	Hirschsprung disease (HSCR) is a birth defect with an approximate incidence of 1/5,000 live births, and up to one-third of HSCR patients develop Hirschsprung-associated enterocolitis (HAEC), the leading cause of HSCR-related death.
31944159	7	39	theme	HSCR	1284:1287	arg1	patients					1289:1296	HSCR patients	1284:1296	HSCR patients	1284:1296	In conclusion, modulating the gut microbiome by encouraging breastfeeding might prevent HAEC progression in HSCR patients.
31944159	1	40	theme	patients	279:286	arg1	one-third					261:269	one-third	261:269	one-third	261:269	Hirschsprung disease (HSCR) is a birth defect with an approximate incidence of 1/5,000 live births, and up to one-third of HSCR patients develop Hirschsprung-associated enterocolitis (HAEC), the leading cause of HSCR-related death.
31944159	1	40	theme	patients	279:286	arg1	patients					279:286	HSCR patients	274:286	HSCR patients	274:286	Hirschsprung disease (HSCR) is a birth defect with an approximate incidence of 1/5,000 live births, and up to one-third of HSCR patients develop Hirschsprung-associated enterocolitis (HAEC), the leading cause of HSCR-related death.
31944159	7	41	theme	gut	1206:1208	arg1	microbiome					1210:1219	the gut microbiome	1202:1219	the gut microbiome	1202:1219	In conclusion, modulating the gut microbiome by encouraging breastfeeding might prevent HAEC progression in HSCR patients.
31944159	5	42	dep	HAEC	870:873	arg1	HAEC					913:916	preoperative and postoperative HAEC	882:916	preoperative and postoperative HAEC combined	882:925	Furthermore, we identified exclusive breastfeeding as a novel protective factor for total HAEC (i.e., preoperative and postoperative HAEC combined).
31944159	4	43	theme	operational	677:687	arg1	OTUs					706:709	OTUs	706:709	OTUs	706:709	We identified a microbiome signature containing 21 operational taxonomic units (OTUs) that can potentially predict postoperative HAEC with ~85% accuracy.
31944159	4	43	theme	operational	677:687	arg1	units					699:703	21 operational taxonomic units	674:703	21 operational taxonomic units (OTUs) that can potentially predict postoperative HAEC with ~85% accuracy	674:777	We identified a microbiome signature containing 21 operational taxonomic units (OTUs) that can potentially predict postoperative HAEC with ~85% accuracy.
31944159	0	44	from	occurrence	67:76	arg1	patients					141:148	Hirschsprung disease (HSCR) patients	113:148	Hirschsprung disease (HSCR) patients	113:148	Prospective study reveals a microbiome signature that predicts the occurrence of post-operative enterocolitis in Hirschsprung disease (HSCR) patients.
31944159	1	45	theme	Hirschsprung-associated	296:318	arg1	HAEC					335:338	HAEC	335:338	HAEC	335:338	Hirschsprung disease (HSCR) is a birth defect with an approximate incidence of 1/5,000 live births, and up to one-third of HSCR patients develop Hirschsprung-associated enterocolitis (HAEC), the leading cause of HSCR-related death.
31944159	1	45	theme	Hirschsprung-associated	296:318	arg1	enterocolitis					320:332	Hirschsprung-associated enterocolitis	296:332	Hirschsprung-associated enterocolitis (HAEC)	296:339	Hirschsprung disease (HSCR) is a birth defect with an approximate incidence of 1/5,000 live births, and up to one-third of HSCR patients develop Hirschsprung-associated enterocolitis (HAEC), the leading cause of HSCR-related death.
31944159	1	45	theme	Hirschsprung-associated	296:318	arg1	cause					354:358	the leading cause	342:358	the leading cause of HSCR-related death	342:380	Hirschsprung disease (HSCR) is a birth defect with an approximate incidence of 1/5,000 live births, and up to one-third of HSCR patients develop Hirschsprung-associated enterocolitis (HAEC), the leading cause of HSCR-related death.
31944159	1	46	theme	approximate	205:215	arg1	incidence					217:225	an approximate incidence	202:225	an approximate incidence of 1/5,000 live births	202:248	Hirschsprung disease (HSCR) is a birth defect with an approximate incidence of 1/5,000 live births, and up to one-third of HSCR patients develop Hirschsprung-associated enterocolitis (HAEC), the leading cause of HSCR-related death.
31944159	6	47	theme	bacteria	1137:1144	arg1	concentrations					1160:1173	lower LPS concentrations	1150:1173	lower LPS concentrations	1150:1173	In addition, we discovered that breastfeeding was associated with a lowered risk for HAEC potentially mediated by modulating the gut microbiome composition characterized by a lower abundance of Gram-negative bacteria and lower LPS concentrations.
31944159	6	47	theme	bacteria	1137:1144	arg1	abundance					1110:1118	a lower abundance	1102:1118	a lower abundance of Gram-negative bacteria	1102:1144	In addition, we discovered that breastfeeding was associated with a lowered risk for HAEC potentially mediated by modulating the gut microbiome composition characterized by a lower abundance of Gram-negative bacteria and lower LPS concentrations.
31944159	4	48	theme	taxonomic	689:697	arg1	OTUs					706:709	OTUs	706:709	OTUs	706:709	We identified a microbiome signature containing 21 operational taxonomic units (OTUs) that can potentially predict postoperative HAEC with ~85% accuracy.
31944159	4	48	theme	taxonomic	689:697	arg1	units					699:703	21 operational taxonomic units	674:703	21 operational taxonomic units (OTUs) that can potentially predict postoperative HAEC with ~85% accuracy	674:777	We identified a microbiome signature containing 21 operational taxonomic units (OTUs) that can potentially predict postoperative HAEC with ~85% accuracy.
31944159	6	49	theme	gut	1058:1060	arg1	composition					1073:1083	the gut microbiome composition	1054:1083	the gut microbiome composition characterized by a lower abundance of Gram-negative bacteria and lower LPS concentrations	1054:1173	In addition, we discovered that breastfeeding was associated with a lowered risk for HAEC potentially mediated by modulating the gut microbiome composition characterized by a lower abundance of Gram-negative bacteria and lower LPS concentrations.
31944159	3	50	used	used	478:481	arg2	we					475:476	we	475:476	we	475:476	Here, we used a prospective study to investigate the enteric microbiome composition at the time of surgery as a predictor for developing postoperative HAEC.
31944159	4	51	theme	microbiome	642:651	arg1	signature					653:661	a microbiome signature	640:661	a microbiome signature containing 21 operational taxonomic units (OTUs) that can potentially predict postoperative HAEC with ~85% accuracy	640:777	We identified a microbiome signature containing 21 operational taxonomic units (OTUs) that can potentially predict postoperative HAEC with ~85% accuracy.
31944159	2	52	theme	HAEC	463:466	arg1	pathogenesis					414:425	pathogenesis	414:425	pathogenesis	414:425	Very little is known about the pathogenesis, prevention, and early diagnosis of HAEC.
31944159	2	52	theme	HAEC	463:466	arg1	diagnosis					450:458	early diagnosis	444:458	early diagnosis	444:458	Very little is known about the pathogenesis, prevention, and early diagnosis of HAEC.
31944159	2	52	theme	HAEC	463:466	arg1	prevention					428:437	prevention	428:437	prevention	428:437	Very little is known about the pathogenesis, prevention, and early diagnosis of HAEC.
31944159	1	53	dep	one-third	261:269	arg1	to					258:259	to	258:259	to	258:259	Hirschsprung disease (HSCR) is a birth defect with an approximate incidence of 1/5,000 live births, and up to one-third of HSCR patients develop Hirschsprung-associated enterocolitis (HAEC), the leading cause of HSCR-related death.
31944159	3	54	theme	surgery	568:574	arg1	time					560:563	the time	556:563	the time of surgery	556:574	Here, we used a prospective study to investigate the enteric microbiome composition at the time of surgery as a predictor for developing postoperative HAEC.
31944159	1	55	theme	Hirschsprung	151:162	arg1	defect					190:195	a birth defect	182:195	a birth defect with an approximate incidence of 1/5,000 live births	182:248	Hirschsprung disease (HSCR) is a birth defect with an approximate incidence of 1/5,000 live births, and up to one-third of HSCR patients develop Hirschsprung-associated enterocolitis (HAEC), the leading cause of HSCR-related death.
31944159	1	55	theme	Hirschsprung	151:162	arg1	HSCR					173:176	HSCR	173:176	HSCR	173:176	Hirschsprung disease (HSCR) is a birth defect with an approximate incidence of 1/5,000 live births, and up to one-third of HSCR patients develop Hirschsprung-associated enterocolitis (HAEC), the leading cause of HSCR-related death.
31944159	1	55	theme	Hirschsprung	151:162	arg1	disease					164:170	Hirschsprung disease	151:170	Hirschsprung disease (HSCR)	151:177	Hirschsprung disease (HSCR) is a birth defect with an approximate incidence of 1/5,000 live births, and up to one-third of HSCR patients develop Hirschsprung-associated enterocolitis (HAEC), the leading cause of HSCR-related death.
33375482	0	0	theme	eupatoria	165:173	arg1	Extract					178:184	Agrimonia eupatoria L. Extract	155:184	Agrimonia eupatoria L. Extract	155:184	Designing New Antibacterial Wound Dressings: Development of a Dual Layer Cotton Material Coated with Poly(Vinyl Alcohol)_Chitosan Nanofibers Incorporating Agrimonia eupatoria L. Extract.
33375482	3	1	theme	medicinal	536:544	arg1	extracts					552:559	medicinal plant extracts	536:559	medicinal plant extracts	536:559	Among the most used antimicrobial agents, medicinal plant extracts demonstrate considerable potential for clinical use, due primarily to their efficacy allied to relatively low incidence of adverse side-effects.
33375482	4	2	theme	Chitosan	895:902	arg1	layer					856:860	a coating layer	846:860	a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG)	846:946	In this context, the present work aimed to develop a unique dual-layer composite material with enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG).
33375482	2	3	contain	containing	461:470	arg1	membranes					451:459	electrospun membranes	439:459	electrospun membranes containing antimicrobial agents	439:491	Therefore, to reduce the risk of wound infections, researchers have expended considerable energy on developing advanced therapeutic dressings, such as electrospun membranes containing antimicrobial agents.
33375482	2	3	contain	containing	461:470	arg2	agents					486:491	antimicrobial agents	472:491	antimicrobial agents	472:491	Therefore, to reduce the risk of wound infections, researchers have expended considerable energy on developing advanced therapeutic dressings, such as electrospun membranes containing antimicrobial agents.
33375482	9	4	theme	cotton/PVA_CS	1727:1739	arg1	material					1750:1757	the TEMPO-oxidized cotton/PVA_CS dressing material	1708:1757	the TEMPO-oxidized cotton/PVA_CS dressing material containing AG extract	1708:1779	In summary, these data provide initial confidence that the TEMPO-oxidized cotton/PVA_CS dressing material containing AG extract demonstrates adequate mechanical attributes for use as a wound dressing and represents a promising approach to prevention of bacterial wound contamination.
33375482	7	5	theme	aeruginosa	1472:1481	arg1	growth					1499:1504	Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) growth	1422:1504	Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) growth	1422:1504	Moreover, antibacterial assays showed that AG-incorporated into PVA_CS's coating layer could effectively inhibit Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) growth.
33375482	6	6	theme	swelling	1290:1297	arg1	capacity					1299:1306	swelling capacity	1290:1306	swelling capacity	1290:1306	The produced dual-layer composite material demonstrated features attractive in production of wound dressings, specifically, wettability, porosity, and swelling capacity.
33375482	3	7	theme	considerable	573:584	arg1	potential					586:594	considerable potential	573:594	considerable potential	573:594	Among the most used antimicrobial agents, medicinal plant extracts demonstrate considerable potential for clinical use, due primarily to their efficacy allied to relatively low incidence of adverse side-effects.
33375482	7	8	dep	aureus	1437:1442	arg1	aureus					1448:1453	S. aureus	1445:1453	S. aureus	1445:1453	Moreover, antibacterial assays showed that AG-incorporated into PVA_CS's coating layer could effectively inhibit Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) growth.
33375482	4	9	theme	eupatoria	930:938	arg1	L					940:940	Agrimonia eupatoria L	920:940	Agrimonia eupatoria L. (AG)	920:946	In this context, the present work aimed to develop a unique dual-layer composite material with enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG).
33375482	4	9	theme	eupatoria	930:938	arg1	AG					944:945	AG	944:945	AG	944:945	In this context, the present work aimed to develop a unique dual-layer composite material with enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG).
33375482	4	10	with	material	787:794	arg1	activity					824:831	enhanced antibacterial activity	801:831	enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG)	801:946	In this context, the present work aimed to develop a unique dual-layer composite material with enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG).
33375482	5	11	theme	2,2,6,6-tetramethylpiperidinyl-1-oxy	1080:1115	arg1	TEMPO					1131:1135	TEMPO	1131:1135	TEMPO	1131:1135	This novel material has properties that facilitate it being electrospun above a conventional cotton gauze bandage pre-treated with 2,2,6,6-tetramethylpiperidinyl-1-oxy free radical (TEMPO).
33375482	5	11	theme	2,2,6,6-tetramethylpiperidinyl-1-oxy	1080:1115	arg1	radical					1122:1128	2,2,6,6-tetramethylpiperidinyl-1-oxy free radical	1080:1128	2,2,6,6-tetramethylpiperidinyl-1-oxy free radical (TEMPO)	1080:1136	This novel material has properties that facilitate it being electrospun above a conventional cotton gauze bandage pre-treated with 2,2,6,6-tetramethylpiperidinyl-1-oxy free radical (TEMPO).
33375482	9	12	theme	mechanical	1803:1812	arg1	attributes					1814:1823	adequate mechanical attributes	1794:1823	adequate mechanical attributes for use as a wound dressing	1794:1851	In summary, these data provide initial confidence that the TEMPO-oxidized cotton/PVA_CS dressing material containing AG extract demonstrates adequate mechanical attributes for use as a wound dressing and represents a promising approach to prevention of bacterial wound contamination.
33375482	0	13	theme	_Chitosan	120:128	arg1	Nanofibers					130:139	Poly(Vinyl Alcohol)_Chitosan Nanofibers	101:139	Poly(Vinyl Alcohol)_Chitosan Nanofibers Incorporating Agrimonia eupatoria L. Extract	101:184	Designing New Antibacterial Wound Dressings: Development of a Dual Layer Cotton Material Coated with Poly(Vinyl Alcohol)_Chitosan Nanofibers Incorporating Agrimonia eupatoria L. Extract.
33375482	5	14	theme	conventional	1029:1040	arg1	bandage					1055:1061	a conventional cotton gauze bandage	1027:1061	a conventional cotton gauze bandage pre-treated with 2,2,6,6-tetramethylpiperidinyl-1-oxy free radical (TEMPO)	1027:1136	This novel material has properties that facilitate it being electrospun above a conventional cotton gauze bandage pre-treated with 2,2,6,6-tetramethylpiperidinyl-1-oxy free radical (TEMPO).
33375482	5	15	theme	gauze	1049:1053	arg1	bandage					1055:1061	a conventional cotton gauze bandage	1027:1061	a conventional cotton gauze bandage pre-treated with 2,2,6,6-tetramethylpiperidinyl-1-oxy free radical (TEMPO)	1027:1136	This novel material has properties that facilitate it being electrospun above a conventional cotton gauze bandage pre-treated with 2,2,6,6-tetramethylpiperidinyl-1-oxy free radical (TEMPO).
33375482	3	16	theme	low	667:669	arg1	incidence					671:679	relatively low incidence	656:679	relatively low incidence of adverse side-effects	656:703	Among the most used antimicrobial agents, medicinal plant extracts demonstrate considerable potential for clinical use, due primarily to their efficacy allied to relatively low incidence of adverse side-effects.
33375482	6	17	theme	dressings	1238:1246	arg1	production					1218:1227	production	1218:1227	production of wound dressings	1218:1246	The produced dual-layer composite material demonstrated features attractive in production of wound dressings, specifically, wettability, porosity, and swelling capacity.
33375482	0	18	theme	Antibacterial	14:26	arg1	Dressings					34:42	New Antibacterial Wound Dressings	10:42	New Antibacterial Wound Dressings	10:42	Designing New Antibacterial Wound Dressings: Development of a Dual Layer Cotton Material Coated with Poly(Vinyl Alcohol)_Chitosan Nanofibers Incorporating Agrimonia eupatoria L. Extract.
33375482	9	19	theme	wound	1838:1842	arg1	dressing					1844:1851	a wound dressing	1836:1851	a wound dressing	1836:1851	In summary, these data provide initial confidence that the TEMPO-oxidized cotton/PVA_CS dressing material containing AG extract demonstrates adequate mechanical attributes for use as a wound dressing and represents a promising approach to prevention of bacterial wound contamination.
33375482	4	20	attach	derived	833:839	arg2	activity					824:831	enhanced antibacterial activity	801:831	enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG)	801:946	In this context, the present work aimed to develop a unique dual-layer composite material with enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG).
33375482	4	20	attach	derived	833:839	arg1	layer					856:860	a coating layer	846:860	a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG)	846:946	In this context, the present work aimed to develop a unique dual-layer composite material with enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG).
33375482	0	21	theme	Layer	67:71	arg1	Material					80:87	a Dual Layer Cotton Material	60:87	a Dual Layer Cotton Material Coated with Poly(Vinyl Alcohol)_Chitosan Nanofibers Incorporating Agrimonia eupatoria L. Extract	60:184	Designing New Antibacterial Wound Dressings: Development of a Dual Layer Cotton Material Coated with Poly(Vinyl Alcohol)_Chitosan Nanofibers Incorporating Agrimonia eupatoria L. Extract.
33375482	4	22	theme	enhanced	801:808	arg1	activity					824:831	enhanced antibacterial activity	801:831	enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG)	801:946	In this context, the present work aimed to develop a unique dual-layer composite material with enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG).
33375482	0	23	theme	Material	80:87	arg1	Development					45:55	Development	45:55	Development of a Dual Layer Cotton Material Coated with Poly(Vinyl Alcohol)_Chitosan Nanofibers Incorporating Agrimonia eupatoria L. Extract	45:184	Designing New Antibacterial Wound Dressings: Development of a Dual Layer Cotton Material Coated with Poly(Vinyl Alcohol)_Chitosan Nanofibers Incorporating Agrimonia eupatoria L. Extract.
33375482	2	24	contain	have	351:354	arg2	energy					378:383	expended considerable energy	356:383	expended considerable energy	356:383	Therefore, to reduce the risk of wound infections, researchers have expended considerable energy on developing advanced therapeutic dressings, such as electrospun membranes containing antimicrobial agents.
33375482	2	24	contain	have	351:354	arg1	researchers					339:349	researchers	339:349	researchers	339:349	Therefore, to reduce the risk of wound infections, researchers have expended considerable energy on developing advanced therapeutic dressings, such as electrospun membranes containing antimicrobial agents.
33375482	5	25	theme	novel	954:958	arg1	material					960:967	This novel material	949:967	This novel material	949:967	This novel material has properties that facilitate it being electrospun above a conventional cotton gauze bandage pre-treated with 2,2,6,6-tetramethylpiperidinyl-1-oxy free radical (TEMPO).
33375482	8	26	theme	material	1566:1573	arg1	profile					1540:1546	the cytotoxic profile	1526:1546	the cytotoxic profile of the dual-layer material in normal human dermal fibroblast (NHDF) cells	1526:1620	Equally important, the cytotoxic profile of the dual-layer material in normal human dermal fibroblast (NHDF) cells demonstrated biocompatibility.
33375482	6	27	from	attractive	1204:1213	arg1	production					1218:1227	production	1218:1227	production of wound dressings	1218:1246	The produced dual-layer composite material demonstrated features attractive in production of wound dressings, specifically, wettability, porosity, and swelling capacity.
33375482	2	28	theme	therapeutic	408:418	arg1	membranes					451:459	electrospun membranes	439:459	electrospun membranes containing antimicrobial agents	439:491	Therefore, to reduce the risk of wound infections, researchers have expended considerable energy on developing advanced therapeutic dressings, such as electrospun membranes containing antimicrobial agents.
33375482	2	28	theme	therapeutic	408:418	arg1	dressings					420:428	advanced therapeutic dressings	399:428	advanced therapeutic dressings	399:428	Therefore, to reduce the risk of wound infections, researchers have expended considerable energy on developing advanced therapeutic dressings, such as electrospun membranes containing antimicrobial agents.
33375482	7	29	theme	coating	1382:1388	arg1	layer					1390:1394	PVA_CS's coating layer	1373:1394	PVA_CS's coating layer	1373:1394	Moreover, antibacterial assays showed that AG-incorporated into PVA_CS's coating layer could effectively inhibit Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) growth.
33375482	2	30	theme	antimicrobial	472:484	arg1	agents					486:491	antimicrobial agents	472:491	antimicrobial agents	472:491	Therefore, to reduce the risk of wound infections, researchers have expended considerable energy on developing advanced therapeutic dressings, such as electrospun membranes containing antimicrobial agents.
33375482	8	31	theme	normal	1578:1583	arg1	cells					1616:1620	normal human dermal fibroblast (NHDF) cells	1578:1620	normal human dermal fibroblast (NHDF) cells	1578:1620	Equally important, the cytotoxic profile of the dual-layer material in normal human dermal fibroblast (NHDF) cells demonstrated biocompatibility.
33375482	4	32	theme	dual-layer	766:775	arg1	material					787:794	a unique dual-layer composite material	757:794	a unique dual-layer composite material with enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG)	757:946	In this context, the present work aimed to develop a unique dual-layer composite material with enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG).
33375482	6	33	from	production	1218:1227	arg1	attractive					1204:1213	attractive	1204:1213	attractive	1204:1213	The produced dual-layer composite material demonstrated features attractive in production of wound dressings, specifically, wettability, porosity, and swelling capacity.
33375482	8	34	theme	dermal	1591:1596	arg1	cells					1616:1620	normal human dermal fibroblast (NHDF) cells	1578:1620	normal human dermal fibroblast (NHDF) cells	1578:1620	Equally important, the cytotoxic profile of the dual-layer material in normal human dermal fibroblast (NHDF) cells demonstrated biocompatibility.
33375482	9	35	contain	containing	1759:1768	arg1	material					1750:1757	the TEMPO-oxidized cotton/PVA_CS dressing material	1708:1757	the TEMPO-oxidized cotton/PVA_CS dressing material containing AG extract	1708:1779	In summary, these data provide initial confidence that the TEMPO-oxidized cotton/PVA_CS dressing material containing AG extract demonstrates adequate mechanical attributes for use as a wound dressing and represents a promising approach to prevention of bacterial wound contamination.
33375482	9	35	contain	containing	1759:1768	arg2	extract					1773:1779	AG extract	1770:1779	AG extract	1770:1779	In summary, these data provide initial confidence that the TEMPO-oxidized cotton/PVA_CS dressing material containing AG extract demonstrates adequate mechanical attributes for use as a wound dressing and represents a promising approach to prevention of bacterial wound contamination.
33375482	8	36	from	profile	1540:1546	arg1	cells					1616:1620	normal human dermal fibroblast (NHDF) cells	1578:1620	normal human dermal fibroblast (NHDF) cells	1578:1620	Equally important, the cytotoxic profile of the dual-layer material in normal human dermal fibroblast (NHDF) cells demonstrated biocompatibility.
33375482	9	37	theme	bacterial	1906:1914	arg1	contamination					1922:1934	bacterial wound contamination	1906:1934	bacterial wound contamination	1906:1934	In summary, these data provide initial confidence that the TEMPO-oxidized cotton/PVA_CS dressing material containing AG extract demonstrates adequate mechanical attributes for use as a wound dressing and represents a promising approach to prevention of bacterial wound contamination.
33375482	3	38	theme	side-effects	692:703	arg1	incidence					671:679	relatively low incidence	656:679	relatively low incidence of adverse side-effects	656:703	Among the most used antimicrobial agents, medicinal plant extracts demonstrate considerable potential for clinical use, due primarily to their efficacy allied to relatively low incidence of adverse side-effects.
33375482	9	39	theme	contamination	1922:1934	arg1	prevention					1892:1901	prevention	1892:1901	prevention of bacterial wound contamination	1892:1934	In summary, these data provide initial confidence that the TEMPO-oxidized cotton/PVA_CS dressing material containing AG extract demonstrates adequate mechanical attributes for use as a wound dressing and represents a promising approach to prevention of bacterial wound contamination.
33375482	3	40	theme	antimicrobial	514:526	arg1	agents					528:533	the most used antimicrobial agents	500:533	the most used antimicrobial agents	500:533	Among the most used antimicrobial agents, medicinal plant extracts demonstrate considerable potential for clinical use, due primarily to their efficacy allied to relatively low incidence of adverse side-effects.
33375482	9	41	theme	dressing	1741:1748	arg1	material					1750:1757	the TEMPO-oxidized cotton/PVA_CS dressing material	1708:1757	the TEMPO-oxidized cotton/PVA_CS dressing material containing AG extract	1708:1779	In summary, these data provide initial confidence that the TEMPO-oxidized cotton/PVA_CS dressing material containing AG extract demonstrates adequate mechanical attributes for use as a wound dressing and represents a promising approach to prevention of bacterial wound contamination.
33375482	6	42	theme	produced	1143:1150	arg1	material					1173:1180	The produced dual-layer composite material	1139:1180	The produced dual-layer composite material	1139:1180	The produced dual-layer composite material demonstrated features attractive in production of wound dressings, specifically, wettability, porosity, and swelling capacity.
33375482	2	43	theme	infections	327:336	arg1	risk					313:316	the risk	309:316	the risk of wound infections	309:336	Therefore, to reduce the risk of wound infections, researchers have expended considerable energy on developing advanced therapeutic dressings, such as electrospun membranes containing antimicrobial agents.
33375482	4	44	theme	coating	848:854	arg1	layer					856:860	a coating layer	846:860	a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG)	846:946	In this context, the present work aimed to develop a unique dual-layer composite material with enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG).
33375482	3	45	theme	plant	546:550	arg1	extracts					552:559	medicinal plant extracts	536:559	medicinal plant extracts	536:559	Among the most used antimicrobial agents, medicinal plant extracts demonstrate considerable potential for clinical use, due primarily to their efficacy allied to relatively low incidence of adverse side-effects.
33375482	0	46	dep	Designing	0:8	arg1	Development					45:55	Development	45:55	Development of a Dual Layer Cotton Material Coated with Poly(Vinyl Alcohol)_Chitosan Nanofibers Incorporating Agrimonia eupatoria L. Extract	45:184	Designing New Antibacterial Wound Dressings: Development of a Dual Layer Cotton Material Coated with Poly(Vinyl Alcohol)_Chitosan Nanofibers Incorporating Agrimonia eupatoria L. Extract.
33375482	0	47	theme	L.	175:176	arg1	Extract					178:184	Agrimonia eupatoria L. Extract	155:184	Agrimonia eupatoria L. Extract	155:184	Designing New Antibacterial Wound Dressings: Development of a Dual Layer Cotton Material Coated with Poly(Vinyl Alcohol)_Chitosan Nanofibers Incorporating Agrimonia eupatoria L. Extract.
33375482	2	48	theme	expended	356:363	arg1	energy					378:383	expended considerable energy	356:383	expended considerable energy	356:383	Therefore, to reduce the risk of wound infections, researchers have expended considerable energy on developing advanced therapeutic dressings, such as electrospun membranes containing antimicrobial agents.
33375482	1	49	theme	pathogenic	267:276	arg1	bacteria					278:285	pathogenic bacteria	267:285	pathogenic bacteria	267:285	Wounds display particular vulnerability to microbial invasion and infections by pathogenic bacteria.
33375482	9	50	theme	TEMPO-oxidized	1712:1725	arg1	material					1750:1757	the TEMPO-oxidized cotton/PVA_CS dressing material	1708:1757	the TEMPO-oxidized cotton/PVA_CS dressing material containing AG extract	1708:1779	In summary, these data provide initial confidence that the TEMPO-oxidized cotton/PVA_CS dressing material containing AG extract demonstrates adequate mechanical attributes for use as a wound dressing and represents a promising approach to prevention of bacterial wound contamination.
33375482	5	51	contain	has	969:971	arg2	properties					973:982	properties	973:982	properties that facilitate it being electrospun above a conventional cotton gauze bandage pre-treated with 2,2,6,6-tetramethylpiperidinyl-1-oxy free radical (TEMPO)	973:1136	This novel material has properties that facilitate it being electrospun above a conventional cotton gauze bandage pre-treated with 2,2,6,6-tetramethylpiperidinyl-1-oxy free radical (TEMPO).
33375482	5	51	contain	has	969:971	arg1	material					960:967	This novel material	949:967	This novel material	949:967	This novel material has properties that facilitate it being electrospun above a conventional cotton gauze bandage pre-treated with 2,2,6,6-tetramethylpiperidinyl-1-oxy free radical (TEMPO).
33375482	4	52	theme	vinyl	870:874	arg1	Poly					865:868	Poly	865:868	Poly(vinyl alcohol) (PVA)	865:889	In this context, the present work aimed to develop a unique dual-layer composite material with enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG).
33375482	4	52	theme	vinyl	870:874	arg1	alcohol					876:882	vinyl alcohol	870:882	vinyl alcohol	870:882	In this context, the present work aimed to develop a unique dual-layer composite material with enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG).
33375482	6	53	theme	attractive	1204:1213	arg1	features					1195:1202	features	1195:1202	features attractive in production of wound dressings	1195:1246	The produced dual-layer composite material demonstrated features attractive in production of wound dressings, specifically, wettability, porosity, and swelling capacity.
33375482	0	54	theme	Vinyl	106:110	arg1	Alcohol					112:118	Vinyl Alcohol	106:118	Vinyl Alcohol	106:118	Designing New Antibacterial Wound Dressings: Development of a Dual Layer Cotton Material Coated with Poly(Vinyl Alcohol)_Chitosan Nanofibers Incorporating Agrimonia eupatoria L. Extract.
33375482	0	54	theme	Vinyl	106:110	arg1	Poly					101:104	Poly	101:104	Poly(Vinyl Alcohol)_Chitosan Nanofibers Incorporating Agrimonia eupatoria L. Extract	101:184	Designing New Antibacterial Wound Dressings: Development of a Dual Layer Cotton Material Coated with Poly(Vinyl Alcohol)_Chitosan Nanofibers Incorporating Agrimonia eupatoria L. Extract.
33375482	3	55	theme	clinical	600:607	arg1	use					609:611	clinical use	600:611	clinical use	600:611	Among the most used antimicrobial agents, medicinal plant extracts demonstrate considerable potential for clinical use, due primarily to their efficacy allied to relatively low incidence of adverse side-effects.
33375482	4	56	theme	Agrimonia	920:928	arg1	L					940:940	Agrimonia eupatoria L	920:940	Agrimonia eupatoria L. (AG)	920:946	In this context, the present work aimed to develop a unique dual-layer composite material with enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG).
33375482	4	56	theme	Agrimonia	920:928	arg1	AG					944:945	AG	944:945	AG	944:945	In this context, the present work aimed to develop a unique dual-layer composite material with enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG).
33375482	6	57	theme	composite	1163:1171	arg1	material					1173:1180	The produced dual-layer composite material	1139:1180	The produced dual-layer composite material	1139:1180	The produced dual-layer composite material demonstrated features attractive in production of wound dressings, specifically, wettability, porosity, and swelling capacity.
33375482	7	58	theme	antibacterial	1319:1331	arg1	assays					1333:1338	antibacterial assays	1319:1338	antibacterial assays	1319:1338	Moreover, antibacterial assays showed that AG-incorporated into PVA_CS's coating layer could effectively inhibit Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) growth.
33375482	8	59	dep	demonstrated	1622:1633	arg1	important					1515:1523	important	1515:1523	important	1515:1523	Equally important, the cytotoxic profile of the dual-layer material in normal human dermal fibroblast (NHDF) cells demonstrated biocompatibility.
33375482	1	60	theme	particular	202:211	arg1	vulnerability					213:225	particular vulnerability	202:225	particular vulnerability to microbial invasion	202:247	Wounds display particular vulnerability to microbial invasion and infections by pathogenic bacteria.
33375482	9	61	theme	adequate	1794:1801	arg1	attributes					1814:1823	adequate mechanical attributes	1794:1823	adequate mechanical attributes for use as a wound dressing	1794:1851	In summary, these data provide initial confidence that the TEMPO-oxidized cotton/PVA_CS dressing material containing AG extract demonstrates adequate mechanical attributes for use as a wound dressing and represents a promising approach to prevention of bacterial wound contamination.
33375482	5	62	theme	free	1117:1120	arg1	TEMPO					1131:1135	TEMPO	1131:1135	TEMPO	1131:1135	This novel material has properties that facilitate it being electrospun above a conventional cotton gauze bandage pre-treated with 2,2,6,6-tetramethylpiperidinyl-1-oxy free radical (TEMPO).
33375482	5	62	theme	free	1117:1120	arg1	radical					1122:1128	2,2,6,6-tetramethylpiperidinyl-1-oxy free radical	1080:1128	2,2,6,6-tetramethylpiperidinyl-1-oxy free radical (TEMPO)	1080:1136	This novel material has properties that facilitate it being electrospun above a conventional cotton gauze bandage pre-treated with 2,2,6,6-tetramethylpiperidinyl-1-oxy free radical (TEMPO).
33375482	7	63	dep	aeruginosa	1472:1481	arg1	aeruginosa					1487:1496	P. aeruginosa	1484:1496	P. aeruginosa	1484:1496	Moreover, antibacterial assays showed that AG-incorporated into PVA_CS's coating layer could effectively inhibit Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) growth.
33375482	9	64	theme	AG	1770:1771	arg1	extract					1773:1779	AG extract	1770:1779	AG extract	1770:1779	In summary, these data provide initial confidence that the TEMPO-oxidized cotton/PVA_CS dressing material containing AG extract demonstrates adequate mechanical attributes for use as a wound dressing and represents a promising approach to prevention of bacterial wound contamination.
33375482	7	65	theme	aureus	1437:1442	arg1	growth					1499:1504	Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) growth	1422:1504	Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) growth	1422:1504	Moreover, antibacterial assays showed that AG-incorporated into PVA_CS's coating layer could effectively inhibit Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) growth.
33375482	0	66	theme	Agrimonia	155:163	arg1	Extract					178:184	Agrimonia eupatoria L. Extract	155:184	Agrimonia eupatoria L. Extract	155:184	Designing New Antibacterial Wound Dressings: Development of a Dual Layer Cotton Material Coated with Poly(Vinyl Alcohol)_Chitosan Nanofibers Incorporating Agrimonia eupatoria L. Extract.
33375482	5	67	theme	cotton	1042:1047	arg1	bandage					1055:1061	a conventional cotton gauze bandage	1027:1061	a conventional cotton gauze bandage pre-treated with 2,2,6,6-tetramethylpiperidinyl-1-oxy free radical (TEMPO)	1027:1136	This novel material has properties that facilitate it being electrospun above a conventional cotton gauze bandage pre-treated with 2,2,6,6-tetramethylpiperidinyl-1-oxy free radical (TEMPO).
33375482	8	68	theme	cytotoxic	1530:1538	arg1	profile					1540:1546	the cytotoxic profile	1526:1546	the cytotoxic profile of the dual-layer material in normal human dermal fibroblast (NHDF) cells	1526:1620	Equally important, the cytotoxic profile of the dual-layer material in normal human dermal fibroblast (NHDF) cells demonstrated biocompatibility.
33375482	0	69	theme	Dual	62:65	arg1	Material					80:87	a Dual Layer Cotton Material	60:87	a Dual Layer Cotton Material Coated with Poly(Vinyl Alcohol)_Chitosan Nanofibers Incorporating Agrimonia eupatoria L. Extract	60:184	Designing New Antibacterial Wound Dressings: Development of a Dual Layer Cotton Material Coated with Poly(Vinyl Alcohol)_Chitosan Nanofibers Incorporating Agrimonia eupatoria L. Extract.
33375482	3	70	theme	allied	646:651	arg1	efficacy					637:644	their efficacy	631:644	their efficacy allied to relatively low incidence of adverse side-effects	631:703	Among the most used antimicrobial agents, medicinal plant extracts demonstrate considerable potential for clinical use, due primarily to their efficacy allied to relatively low incidence of adverse side-effects.
33375482	6	71	theme	wound	1232:1236	arg1	dressings					1238:1246	wound dressings	1232:1246	wound dressings	1232:1246	The produced dual-layer composite material demonstrated features attractive in production of wound dressings, specifically, wettability, porosity, and swelling capacity.
33375482	0	72	theme	Cotton	73:78	arg1	Material					80:87	a Dual Layer Cotton Material	60:87	a Dual Layer Cotton Material Coated with Poly(Vinyl Alcohol)_Chitosan Nanofibers Incorporating Agrimonia eupatoria L. Extract	60:184	Designing New Antibacterial Wound Dressings: Development of a Dual Layer Cotton Material Coated with Poly(Vinyl Alcohol)_Chitosan Nanofibers Incorporating Agrimonia eupatoria L. Extract.
33375482	8	73	theme	dual-layer	1555:1564	arg1	material					1566:1573	the dual-layer material	1551:1573	the dual-layer material	1551:1573	Equally important, the cytotoxic profile of the dual-layer material in normal human dermal fibroblast (NHDF) cells demonstrated biocompatibility.
33375482	4	74	theme	present	727:733	arg1	work					735:738	the present work	723:738	the present work	723:738	In this context, the present work aimed to develop a unique dual-layer composite material with enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG).
33375482	2	75	theme	wound	321:325	arg1	infections					327:336	wound infections	321:336	wound infections	321:336	Therefore, to reduce the risk of wound infections, researchers have expended considerable energy on developing advanced therapeutic dressings, such as electrospun membranes containing antimicrobial agents.
33375482	0	76	theme	Poly	101:104	arg1	Nanofibers					130:139	Poly(Vinyl Alcohol)_Chitosan Nanofibers	101:139	Poly(Vinyl Alcohol)_Chitosan Nanofibers Incorporating Agrimonia eupatoria L. Extract	101:184	Designing New Antibacterial Wound Dressings: Development of a Dual Layer Cotton Material Coated with Poly(Vinyl Alcohol)_Chitosan Nanofibers Incorporating Agrimonia eupatoria L. Extract.
33375482	4	77	contain	containing	909:918	arg2	L					940:940	Agrimonia eupatoria L	920:940	Agrimonia eupatoria L. (AG)	920:946	In this context, the present work aimed to develop a unique dual-layer composite material with enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG).
33375482	4	77	contain	containing	909:918	arg1	CS					905:906	CS	905:906	CS	905:906	In this context, the present work aimed to develop a unique dual-layer composite material with enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG).
33375482	4	77	contain	containing	909:918	arg2	AG					944:945	AG	944:945	AG	944:945	In this context, the present work aimed to develop a unique dual-layer composite material with enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG).
33375482	4	77	contain	containing	909:918	arg1	Chitosan					895:902	Chitosan	895:902	Chitosan (CS) containing Agrimonia eupatoria L. (AG)	895:946	In this context, the present work aimed to develop a unique dual-layer composite material with enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG).
33375482	9	78	theme	promising	1870:1878	arg1	approach					1880:1887	a promising approach	1868:1887	a promising approach to prevention of bacterial wound contamination	1868:1934	In summary, these data provide initial confidence that the TEMPO-oxidized cotton/PVA_CS dressing material containing AG extract demonstrates adequate mechanical attributes for use as a wound dressing and represents a promising approach to prevention of bacterial wound contamination.
33375482	8	79	theme	human	1585:1589	arg1	cells					1616:1620	normal human dermal fibroblast (NHDF) cells	1578:1620	normal human dermal fibroblast (NHDF) cells	1578:1620	Equally important, the cytotoxic profile of the dual-layer material in normal human dermal fibroblast (NHDF) cells demonstrated biocompatibility.
33375482	4	80	theme	antibacterial	810:822	arg1	activity					824:831	enhanced antibacterial activity	801:831	enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG)	801:946	In this context, the present work aimed to develop a unique dual-layer composite material with enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG).
33375482	2	81	theme	advanced	399:406	arg1	membranes					451:459	electrospun membranes	439:459	electrospun membranes containing antimicrobial agents	439:491	Therefore, to reduce the risk of wound infections, researchers have expended considerable energy on developing advanced therapeutic dressings, such as electrospun membranes containing antimicrobial agents.
33375482	2	81	theme	advanced	399:406	arg1	dressings					420:428	advanced therapeutic dressings	399:428	advanced therapeutic dressings	399:428	Therefore, to reduce the risk of wound infections, researchers have expended considerable energy on developing advanced therapeutic dressings, such as electrospun membranes containing antimicrobial agents.
33375482	0	82	theme	New	10:12	arg1	Dressings					34:42	New Antibacterial Wound Dressings	10:42	New Antibacterial Wound Dressings	10:42	Designing New Antibacterial Wound Dressings: Development of a Dual Layer Cotton Material Coated with Poly(Vinyl Alcohol)_Chitosan Nanofibers Incorporating Agrimonia eupatoria L. Extract.
33375482	8	83	theme	fibroblast	1598:1607	arg1	cells					1616:1620	normal human dermal fibroblast (NHDF) cells	1578:1620	normal human dermal fibroblast (NHDF) cells	1578:1620	Equally important, the cytotoxic profile of the dual-layer material in normal human dermal fibroblast (NHDF) cells demonstrated biocompatibility.
33375482	4	84	theme	unique	759:764	arg1	material					787:794	a unique dual-layer composite material	757:794	a unique dual-layer composite material with enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG)	757:946	In this context, the present work aimed to develop a unique dual-layer composite material with enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG).
33375482	0	85	theme	Wound	28:32	arg1	Dressings					34:42	New Antibacterial Wound Dressings	10:42	New Antibacterial Wound Dressings	10:42	Designing New Antibacterial Wound Dressings: Development of a Dual Layer Cotton Material Coated with Poly(Vinyl Alcohol)_Chitosan Nanofibers Incorporating Agrimonia eupatoria L. Extract.
33375482	2	86	theme	electrospun	439:449	arg1	membranes					451:459	electrospun membranes	439:459	electrospun membranes containing antimicrobial agents	439:491	Therefore, to reduce the risk of wound infections, researchers have expended considerable energy on developing advanced therapeutic dressings, such as electrospun membranes containing antimicrobial agents.
33375482	8	87	theme	NHDF	1610:1613	arg1	cells					1616:1620	normal human dermal fibroblast (NHDF) cells	1578:1620	normal human dermal fibroblast (NHDF) cells	1578:1620	Equally important, the cytotoxic profile of the dual-layer material in normal human dermal fibroblast (NHDF) cells demonstrated biocompatibility.
33375482	3	88	theme	adverse	684:690	arg1	side-effects					692:703	adverse side-effects	684:703	adverse side-effects	684:703	Among the most used antimicrobial agents, medicinal plant extracts demonstrate considerable potential for clinical use, due primarily to their efficacy allied to relatively low incidence of adverse side-effects.
33375482	4	89	theme	composite	777:785	arg1	material					787:794	a unique dual-layer composite material	757:794	a unique dual-layer composite material with enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG)	757:946	In this context, the present work aimed to develop a unique dual-layer composite material with enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG).
33375482	9	90	theme	wound	1916:1920	arg1	contamination					1922:1934	bacterial wound contamination	1906:1934	bacterial wound contamination	1906:1934	In summary, these data provide initial confidence that the TEMPO-oxidized cotton/PVA_CS dressing material containing AG extract demonstrates adequate mechanical attributes for use as a wound dressing and represents a promising approach to prevention of bacterial wound contamination.
33375482	6	91	theme	dual-layer	1152:1161	arg1	material					1173:1180	The produced dual-layer composite material	1139:1180	The produced dual-layer composite material	1139:1180	The produced dual-layer composite material demonstrated features attractive in production of wound dressings, specifically, wettability, porosity, and swelling capacity.
33375482	4	92	theme	Poly	865:868	arg1	layer					856:860	a coating layer	846:860	a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG)	846:946	In this context, the present work aimed to develop a unique dual-layer composite material with enhanced antibacterial activity derived from a coating layer of Poly(vinyl alcohol) (PVA) and Chitosan (CS) containing Agrimonia eupatoria L. (AG).
33375482	9	93	theme	initial	1684:1690	arg1	confidence					1692:1701	initial confidence that the TEMPO-oxidized cotton/PVA_CS dressing material containing AG extract demonstrates adequate mechanical attributes for use as a wound dressing and represents a promising approach to prevention of bacterial wound contamination	1684:1934	initial confidence that the TEMPO-oxidized cotton/PVA_CS dressing material containing AG extract demonstrates adequate mechanical attributes for use as a wound dressing and represents a promising approach to prevention of bacterial wound contamination	1684:1934	In summary, these data provide initial confidence that the TEMPO-oxidized cotton/PVA_CS dressing material containing AG extract demonstrates adequate mechanical attributes for use as a wound dressing and represents a promising approach to prevention of bacterial wound contamination.
33375482	3	94	theme	used	509:512	arg1	agents					528:533	the most used antimicrobial agents	500:533	the most used antimicrobial agents	500:533	Among the most used antimicrobial agents, medicinal plant extracts demonstrate considerable potential for clinical use, due primarily to their efficacy allied to relatively low incidence of adverse side-effects.
33375482	1	95	theme	microbial	230:238	arg1	invasion					240:247	microbial invasion	230:247	microbial invasion	230:247	Wounds display particular vulnerability to microbial invasion and infections by pathogenic bacteria.
33375482	2	96	theme	considerable	365:376	arg1	energy					378:383	expended considerable energy	356:383	expended considerable energy	356:383	Therefore, to reduce the risk of wound infections, researchers have expended considerable energy on developing advanced therapeutic dressings, such as electrospun membranes containing antimicrobial agents.
31982530	6	0	theme	GFPS-based	1008:1017	arg1	complex					1019:1025	This GFPS-based complex	1003:1025	This GFPS-based complex	1003:1025	This GFPS-based complex could incorporate TNFα-A60 into the macrophage cells via dectin-1 receptor and attenuate lipopolysaccharide-induced secretion of TNFα.
31982530	3	1	theme	-linked	609:615	arg1	residue					632:638	a single β-D-(1-6)-linked glucopyranosyl residue	591:638	a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue	591:677	The structure of GFPS was determined with FT-IR, NMR, and monosaccharide composition analysis, and was identified to be a β-D-(1-3)-linked glucan backbone with a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue.
31982530	5	2	theme	cytokine	977:984	arg1	TNFα-A60					992:999	TNFα-A60	992:999	TNFα-A60	992:999	Further studies demonstrated that GFPS could interact with poly[A] moiety of a designed antisense oligonucleotide (ASO) targeting the primary transcript of proinflammatory cytokine TNFα (TNFα-A60).
31982530	5	2	theme	cytokine	977:984	arg1	TNFα					986:989	proinflammatory cytokine TNFα	961:989	proinflammatory cytokine TNFα (TNFα-A60)	961:1000	Further studies demonstrated that GFPS could interact with poly[A] moiety of a designed antisense oligonucleotide (ASO) targeting the primary transcript of proinflammatory cytokine TNFα (TNFα-A60).
31982530	2	3	theme	5.42 × 106 Da	342:354	arg1	mass					334:337	high molecular mass	319:337	high molecular mass of 5.42 × 106 Da	319:354	In the present study, a soluble homogeneous β-glucan, GFPS, with high molecular mass of 5.42 × 106 Da was purified from the fruit bodies of Grifola frondosa using 5% cold NaOH.
31982530	5	4	theme	antisense	893:901	arg1	ASO					920:922	ASO	920:922	ASO	920:922	Further studies demonstrated that GFPS could interact with poly[A] moiety of a designed antisense oligonucleotide (ASO) targeting the primary transcript of proinflammatory cytokine TNFα (TNFα-A60).
31982530	5	4	theme	antisense	893:901	arg1	oligonucleotide					903:917	a designed antisense oligonucleotide	882:917	a designed antisense oligonucleotide (ASO) targeting the primary transcript of proinflammatory cytokine TNFα (TNFα-A60)	882:1000	Further studies demonstrated that GFPS could interact with poly[A] moiety of a designed antisense oligonucleotide (ASO) targeting the primary transcript of proinflammatory cytokine TNFα (TNFα-A60).
31982530	3	5	theme	glucopyranosyl	617:630	arg1	residue					632:638	a single β-D-(1-6)-linked glucopyranosyl residue	591:638	a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue	591:677	The structure of GFPS was determined with FT-IR, NMR, and monosaccharide composition analysis, and was identified to be a β-D-(1-3)-linked glucan backbone with a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue.
31982530	6	6	theme	lipopolysaccharide-induced	1116:1141	arg1	secretion					1143:1151	lipopolysaccharide-induced secretion	1116:1151	lipopolysaccharide-induced secretion of TNFα	1116:1159	This GFPS-based complex could incorporate TNFα-A60 into the macrophage cells via dectin-1 receptor and attenuate lipopolysaccharide-induced secretion of TNFα.
31982530	0	7	theme	dectin-1	97:104	arg1	receptor					106:113	dectin-1 receptor	97:113	dectin-1 receptor	97:113	A β-glucan from Grifola frondosa effectively delivers therapeutic oligonucleotide into cells via dectin-1 receptor and attenuates TNFα gene expression.
31982530	6	8	theme	TNFα	1156:1159	arg1	secretion					1143:1151	lipopolysaccharide-induced secretion	1116:1151	lipopolysaccharide-induced secretion of TNFα	1116:1159	This GFPS-based complex could incorporate TNFα-A60 into the macrophage cells via dectin-1 receptor and attenuate lipopolysaccharide-induced secretion of TNFα.
31982530	2	9	theme	homogeneous	286:296	arg1	GFPS					308:311	GFPS	308:311	GFPS	308:311	In the present study, a soluble homogeneous β-glucan, GFPS, with high molecular mass of 5.42 × 106 Da was purified from the fruit bodies of Grifola frondosa using 5% cold NaOH.
31982530	2	9	theme	homogeneous	286:296	arg1	β-glucan					298:305	a soluble homogeneous β-glucan	276:305	a soluble homogeneous β-glucan	276:305	In the present study, a soluble homogeneous β-glucan, GFPS, with high molecular mass of 5.42 × 106 Da was purified from the fruit bodies of Grifola frondosa using 5% cold NaOH.
31982530	3	10	theme	GFPS	448:451	arg1	structure					435:443	The structure	431:443	The structure of GFPS	431:451	The structure of GFPS was determined with FT-IR, NMR, and monosaccharide composition analysis, and was identified to be a β-D-(1-3)-linked glucan backbone with a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue.
31982530	3	10	theme	GFPS	448:451	arg1	backbone					577:584	a β-D-(1-3)-linked glucan backbone	551:584	a β-D-(1-3)-linked glucan backbone with a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue	551:677	The structure of GFPS was determined with FT-IR, NMR, and monosaccharide composition analysis, and was identified to be a β-D-(1-3)-linked glucan backbone with a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue.
31982530	2	11	theme	soluble	278:284	arg1	GFPS					308:311	GFPS	308:311	GFPS	308:311	In the present study, a soluble homogeneous β-glucan, GFPS, with high molecular mass of 5.42 × 106 Da was purified from the fruit bodies of Grifola frondosa using 5% cold NaOH.
31982530	2	11	theme	soluble	278:284	arg1	β-glucan					298:305	a soluble homogeneous β-glucan	276:305	a soluble homogeneous β-glucan	276:305	In the present study, a soluble homogeneous β-glucan, GFPS, with high molecular mass of 5.42 × 106 Da was purified from the fruit bodies of Grifola frondosa using 5% cold NaOH.
31982530	2	12	theme	fruit	378:382	arg1	bodies					384:389	the fruit bodies	374:389	the fruit bodies of Grifola frondosa	374:409	In the present study, a soluble homogeneous β-glucan, GFPS, with high molecular mass of 5.42 × 106 Da was purified from the fruit bodies of Grifola frondosa using 5% cold NaOH.
31982530	1	13	theme	edible	175:180	arg1	frondosa					160:167	Grifola frondosa	152:167	Grifola frondosa	152:167	Grifola frondosa is an edible and medicinal mushroom with great nutritional values and bioactivities.
31982530	1	13	theme	edible	175:180	arg1	mushroom					196:203	an edible and medicinal mushroom	172:203	mushroom	196:203	Grifola frondosa is an edible and medicinal mushroom with great nutritional values and bioactivities.
31982530	3	14	theme	composition	504:514	arg1	analysis					516:523	monosaccharide composition analysis	489:523	monosaccharide composition analysis	489:523	The structure of GFPS was determined with FT-IR, NMR, and monosaccharide composition analysis, and was identified to be a β-D-(1-3)-linked glucan backbone with a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue.
31982530	0	15	from	frondosa	24:31	arg1	β-glucan					2:9	A β-glucan	0:9	A β-glucan from Grifola frondosa	0:31	A β-glucan from Grifola frondosa effectively delivers therapeutic oligonucleotide into cells via dectin-1 receptor and attenuates TNFα gene expression.
31982530	6	16	theme	dectin-1	1084:1091	arg1	receptor					1093:1100	dectin-1 receptor	1084:1100	dectin-1 receptor	1084:1100	This GFPS-based complex could incorporate TNFα-A60 into the macrophage cells via dectin-1 receptor and attenuate lipopolysaccharide-induced secretion of TNFα.
31982530	3	17	theme	monosaccharide	489:502	arg1	analysis					516:523	monosaccharide composition analysis	489:523	monosaccharide composition analysis	489:523	The structure of GFPS was determined with FT-IR, NMR, and monosaccharide composition analysis, and was identified to be a β-D-(1-3)-linked glucan backbone with a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue.
31982530	1	18	theme	medicinal	186:194	arg1	frondosa					160:167	Grifola frondosa	152:167	Grifola frondosa	152:167	Grifola frondosa is an edible and medicinal mushroom with great nutritional values and bioactivities.
31982530	1	18	theme	medicinal	186:194	arg1	mushroom					196:203	an edible and medicinal mushroom	172:203	mushroom	196:203	Grifola frondosa is an edible and medicinal mushroom with great nutritional values and bioactivities.
31982530	4	19	theme	polydeoxyadenylic	771:787	arg1	poly[A					795:800	poly[A	795:800	poly[A	795:800	Our results indicated that GFPS had a triple helical structure and could form complex with polydeoxyadenylic acid (poly[A]).
31982530	4	19	theme	polydeoxyadenylic	771:787	arg1	acid					789:792	polydeoxyadenylic acid	771:792	polydeoxyadenylic acid (poly[A])	771:802	Our results indicated that GFPS had a triple helical structure and could form complex with polydeoxyadenylic acid (poly[A]).
31982530	5	20	theme	poly[A	864:869	arg1	moiety					872:877	poly[A] moiety	864:877	poly[A] moiety of a designed antisense oligonucleotide (ASO) targeting the primary transcript of proinflammatory cytokine TNFα (TNFα-A60)	864:1000	Further studies demonstrated that GFPS could interact with poly[A] moiety of a designed antisense oligonucleotide (ASO) targeting the primary transcript of proinflammatory cytokine TNFα (TNFα-A60).
31982530	3	21	link	-linked	609:615	arg1	residue					632:638	a single β-D-(1-6)-linked glucopyranosyl residue	591:638	a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue	591:677	The structure of GFPS was determined with FT-IR, NMR, and monosaccharide composition analysis, and was identified to be a β-D-(1-3)-linked glucan backbone with a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue.
31982530	3	22	theme	single	593:598	arg1	residue					632:638	a single β-D-(1-6)-linked glucopyranosyl residue	591:638	a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue	591:677	The structure of GFPS was determined with FT-IR, NMR, and monosaccharide composition analysis, and was identified to be a β-D-(1-3)-linked glucan backbone with a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue.
31982530	2	23	theme	cold	420:423	arg1	NaOH					425:428	5% cold NaOH	417:428	5% cold NaOH	417:428	In the present study, a soluble homogeneous β-glucan, GFPS, with high molecular mass of 5.42 × 106 Da was purified from the fruit bodies of Grifola frondosa using 5% cold NaOH.
31982530	4	24	theme	helical	725:731	arg1	structure					733:741	a triple helical structure	716:741	a triple helical structure	716:741	Our results indicated that GFPS had a triple helical structure and could form complex with polydeoxyadenylic acid (poly[A]).
31982530	0	25	theme	gene	135:138	arg1	expression					140:149	TNFα gene expression	130:149	TNFα gene expression	130:149	A β-glucan from Grifola frondosa effectively delivers therapeutic oligonucleotide into cells via dectin-1 receptor and attenuates TNFα gene expression.
31982530	3	26	link	-linked	562:568	arg1	structure					435:443	The structure	431:443	The structure of GFPS	431:451	The structure of GFPS was determined with FT-IR, NMR, and monosaccharide composition analysis, and was identified to be a β-D-(1-3)-linked glucan backbone with a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue.
31982530	3	26	link	-linked	562:568	arg1	backbone					577:584	a β-D-(1-3)-linked glucan backbone	551:584	a β-D-(1-3)-linked glucan backbone with a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue	551:677	The structure of GFPS was determined with FT-IR, NMR, and monosaccharide composition analysis, and was identified to be a β-D-(1-3)-linked glucan backbone with a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue.
31982530	2	27	theme	5	417:417	arg1	%					418:418	%	418:418	%	418:418	In the present study, a soluble homogeneous β-glucan, GFPS, with high molecular mass of 5.42 × 106 Da was purified from the fruit bodies of Grifola frondosa using 5% cold NaOH.
31982530	2	28	theme	%	418:418	arg1	NaOH					425:428	5% cold NaOH	417:428	5% cold NaOH	417:428	In the present study, a soluble homogeneous β-glucan, GFPS, with high molecular mass of 5.42 × 106 Da was purified from the fruit bodies of Grifola frondosa using 5% cold NaOH.
31982530	3	29	theme	third	665:669	arg1	residue					671:677	every third residue	659:677	every third residue	659:677	The structure of GFPS was determined with FT-IR, NMR, and monosaccharide composition analysis, and was identified to be a β-D-(1-3)-linked glucan backbone with a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue.
31982530	0	30	theme	TNFα	130:133	arg1	expression					140:149	TNFα gene expression	130:149	TNFα gene expression	130:149	A β-glucan from Grifola frondosa effectively delivers therapeutic oligonucleotide into cells via dectin-1 receptor and attenuates TNFα gene expression.
31982530	6	31	theme	macrophage	1063:1072	arg1	cells					1074:1078	the macrophage cells	1059:1078	the macrophage cells	1059:1078	This GFPS-based complex could incorporate TNFα-A60 into the macrophage cells via dectin-1 receptor and attenuate lipopolysaccharide-induced secretion of TNFα.
31982530	1	32	theme	great	210:214	arg1	values					228:233	great nutritional values	210:233	great nutritional values	210:233	Grifola frondosa is an edible and medicinal mushroom with great nutritional values and bioactivities.
31982530	1	33	theme	nutritional	216:226	arg1	values					228:233	great nutritional values	210:233	great nutritional values	210:233	Grifola frondosa is an edible and medicinal mushroom with great nutritional values and bioactivities.
31982530	2	34	theme	frondosa	402:409	arg1	bodies					384:389	the fruit bodies	374:389	the fruit bodies of Grifola frondosa	374:409	In the present study, a soluble homogeneous β-glucan, GFPS, with high molecular mass of 5.42 × 106 Da was purified from the fruit bodies of Grifola frondosa using 5% cold NaOH.
31982530	0	35	theme	Grifola	16:22	arg1	frondosa					24:31	Grifola frondosa	16:31	Grifola frondosa	16:31	A β-glucan from Grifola frondosa effectively delivers therapeutic oligonucleotide into cells via dectin-1 receptor and attenuates TNFα gene expression.
31982530	0	36	theme	therapeutic	54:64	arg1	oligonucleotide					66:80	therapeutic oligonucleotide	54:80	therapeutic oligonucleotide into cells	54:91	A β-glucan from Grifola frondosa effectively delivers therapeutic oligonucleotide into cells via dectin-1 receptor and attenuates TNFα gene expression.
31982530	5	37	theme	designed	884:891	arg1	ASO					920:922	ASO	920:922	ASO	920:922	Further studies demonstrated that GFPS could interact with poly[A] moiety of a designed antisense oligonucleotide (ASO) targeting the primary transcript of proinflammatory cytokine TNFα (TNFα-A60).
31982530	5	37	theme	designed	884:891	arg1	oligonucleotide					903:917	a designed antisense oligonucleotide	882:917	a designed antisense oligonucleotide (ASO) targeting the primary transcript of proinflammatory cytokine TNFα (TNFα-A60)	882:1000	Further studies demonstrated that GFPS could interact with poly[A] moiety of a designed antisense oligonucleotide (ASO) targeting the primary transcript of proinflammatory cytokine TNFα (TNFα-A60).
31982530	5	38	theme	primary	939:945	arg1	transcript					947:956	the primary transcript	935:956	the primary transcript of proinflammatory cytokine TNFα (TNFα-A60)	935:1000	Further studies demonstrated that GFPS could interact with poly[A] moiety of a designed antisense oligonucleotide (ASO) targeting the primary transcript of proinflammatory cytokine TNFα (TNFα-A60).
31982530	7	39	theme	therapeutic	1222:1232	arg1	oligonucleotides					1234:1249	therapeutic oligonucleotides	1222:1249	therapeutic oligonucleotides	1222:1249	Our results suggested that GFPS could be applied to deliver therapeutic oligonucleotides for the treatment of diseases such as inflammation and cancers.
31982530	1	40	with	mushroom	196:203	arg1	bioactivities					239:251	bioactivities	239:251	bioactivities	239:251	Grifola frondosa is an edible and medicinal mushroom with great nutritional values and bioactivities.
31982530	1	40	with	mushroom	196:203	arg1	values					228:233	great nutritional values	210:233	great nutritional values	210:233	Grifola frondosa is an edible and medicinal mushroom with great nutritional values and bioactivities.
31982530	3	41	theme	1-6	605:607	arg1	residue					632:638	a single β-D-(1-6)-linked glucopyranosyl residue	591:638	a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue	591:677	The structure of GFPS was determined with FT-IR, NMR, and monosaccharide composition analysis, and was identified to be a β-D-(1-3)-linked glucan backbone with a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue.
31982530	5	42	theme	proinflammatory	961:975	arg1	TNFα-A60					992:999	TNFα-A60	992:999	TNFα-A60	992:999	Further studies demonstrated that GFPS could interact with poly[A] moiety of a designed antisense oligonucleotide (ASO) targeting the primary transcript of proinflammatory cytokine TNFα (TNFα-A60).
31982530	5	42	theme	proinflammatory	961:975	arg1	TNFα					986:989	proinflammatory cytokine TNFα	961:989	proinflammatory cytokine TNFα (TNFα-A60)	961:1000	Further studies demonstrated that GFPS could interact with poly[A] moiety of a designed antisense oligonucleotide (ASO) targeting the primary transcript of proinflammatory cytokine TNFα (TNFα-A60).
31982530	5	43	theme	oligonucleotide	903:917	arg1	moiety					872:877	poly[A] moiety	864:877	poly[A] moiety of a designed antisense oligonucleotide (ASO) targeting the primary transcript of proinflammatory cytokine TNFα (TNFα-A60)	864:1000	Further studies demonstrated that GFPS could interact with poly[A] moiety of a designed antisense oligonucleotide (ASO) targeting the primary transcript of proinflammatory cytokine TNFα (TNFα-A60).
31982530	3	44	theme	-linked	562:568	arg1	structure					435:443	The structure	431:443	The structure of GFPS	431:451	The structure of GFPS was determined with FT-IR, NMR, and monosaccharide composition analysis, and was identified to be a β-D-(1-3)-linked glucan backbone with a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue.
31982530	3	44	theme	-linked	562:568	arg1	backbone					577:584	a β-D-(1-3)-linked glucan backbone	551:584	a β-D-(1-3)-linked glucan backbone with a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue	551:677	The structure of GFPS was determined with FT-IR, NMR, and monosaccharide composition analysis, and was identified to be a β-D-(1-3)-linked glucan backbone with a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue.
31982530	2	45	theme	molecular	324:332	arg1	mass					334:337	high molecular mass	319:337	high molecular mass of 5.42 × 106 Da	319:354	In the present study, a soluble homogeneous β-glucan, GFPS, with high molecular mass of 5.42 × 106 Da was purified from the fruit bodies of Grifola frondosa using 5% cold NaOH.
31982530	4	46	contain	had	712:714	arg2	structure					733:741	a triple helical structure	716:741	a triple helical structure	716:741	Our results indicated that GFPS had a triple helical structure and could form complex with polydeoxyadenylic acid (poly[A]).
31982530	4	46	contain	had	712:714	arg1	GFPS					707:710	GFPS	707:710	GFPS	707:710	Our results indicated that GFPS had a triple helical structure and could form complex with polydeoxyadenylic acid (poly[A]).
31982530	3	47	with	backbone	577:584	arg1	residue					632:638	a single β-D-(1-6)-linked glucopyranosyl residue	591:638	a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue	591:677	The structure of GFPS was determined with FT-IR, NMR, and monosaccharide composition analysis, and was identified to be a β-D-(1-3)-linked glucan backbone with a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue.
31982530	3	48	theme	β-D-	600:603	arg1	residue					632:638	a single β-D-(1-6)-linked glucopyranosyl residue	591:638	a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue	591:677	The structure of GFPS was determined with FT-IR, NMR, and monosaccharide composition analysis, and was identified to be a β-D-(1-3)-linked glucan backbone with a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue.
31982530	3	49	theme	glucan	570:575	arg1	structure					435:443	The structure	431:443	The structure of GFPS	431:451	The structure of GFPS was determined with FT-IR, NMR, and monosaccharide composition analysis, and was identified to be a β-D-(1-3)-linked glucan backbone with a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue.
31982530	3	49	theme	glucan	570:575	arg1	backbone					577:584	a β-D-(1-3)-linked glucan backbone	551:584	a β-D-(1-3)-linked glucan backbone with a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue	551:677	The structure of GFPS was determined with FT-IR, NMR, and monosaccharide composition analysis, and was identified to be a β-D-(1-3)-linked glucan backbone with a single β-D-(1-6)-linked glucopyranosyl residue branched at C-6 on every third residue.
31982530	2	50	theme	high	319:322	arg1	mass					334:337	high molecular mass	319:337	high molecular mass of 5.42 × 106 Da	319:354	In the present study, a soluble homogeneous β-glucan, GFPS, with high molecular mass of 5.42 × 106 Da was purified from the fruit bodies of Grifola frondosa using 5% cold NaOH.
31982530	7	51	theme	diseases	1272:1279	arg1	treatment					1259:1267	the treatment	1255:1267	the treatment of diseases such as inflammation and cancers	1255:1312	Our results suggested that GFPS could be applied to deliver therapeutic oligonucleotides for the treatment of diseases such as inflammation and cancers.
31982530	5	52	theme	TNFα	986:989	arg1	transcript					947:956	the primary transcript	935:956	the primary transcript of proinflammatory cytokine TNFα (TNFα-A60)	935:1000	Further studies demonstrated that GFPS could interact with poly[A] moiety of a designed antisense oligonucleotide (ASO) targeting the primary transcript of proinflammatory cytokine TNFα (TNFα-A60).
31982530	2	53	theme	present	261:267	arg1	study					269:273	the present study	257:273	the present study	257:273	In the present study, a soluble homogeneous β-glucan, GFPS, with high molecular mass of 5.42 × 106 Da was purified from the fruit bodies of Grifola frondosa using 5% cold NaOH.
31982530	1	54	theme	Grifola	152:158	arg1	frondosa					160:167	Grifola frondosa	152:167	Grifola frondosa	152:167	Grifola frondosa is an edible and medicinal mushroom with great nutritional values and bioactivities.
31982530	1	54	theme	Grifola	152:158	arg1	mushroom					196:203	an edible and medicinal mushroom	172:203	mushroom	196:203	Grifola frondosa is an edible and medicinal mushroom with great nutritional values and bioactivities.
31982530	2	55	with	β-glucan	298:305	arg1	mass					334:337	high molecular mass	319:337	high molecular mass of 5.42 × 106 Da	319:354	In the present study, a soluble homogeneous β-glucan, GFPS, with high molecular mass of 5.42 × 106 Da was purified from the fruit bodies of Grifola frondosa using 5% cold NaOH.
31982530	4	56	theme	triple	718:723	arg1	structure					733:741	a triple helical structure	716:741	a triple helical structure	716:741	Our results indicated that GFPS had a triple helical structure and could form complex with polydeoxyadenylic acid (poly[A]).
31982530	5	57	theme	Further	805:811	arg1	studies					813:819	Further studies	805:819	Further studies	805:819	Further studies demonstrated that GFPS could interact with poly[A] moiety of a designed antisense oligonucleotide (ASO) targeting the primary transcript of proinflammatory cytokine TNFα (TNFα-A60).
34065932	3	0	theme	population	699:708	arg1	fractionation					671:683	fractionation	671:683	fractionation of the pectin population	671:708	On the one hand, pectin is extracted stepwise using hot water, chelating agents and low-alkaline conditions targeting fractionation of the pectin population.
34065932	0	1	theme	Acid	186:189	arg1	Extraction					191:200	High-Pressure Homogenization-Facilitated Acid Extraction	145:200	High-Pressure Homogenization-Facilitated Acid Extraction	145:200	The Structure and Composition of Extracted Pectin and Residual Cell Wall Material from Processing Tomato: The Role of a Stepwise Approach versus High-Pressure Homogenization-Facilitated Acid Extraction.
34065932	2	2	theme	tomato	355:360	arg1	tomato					355:360	processing tomato	344:360	processing tomato	344:360	In this study, two approaches starting from the alcohol-insoluble residue (AIR) of processing tomato are performed in a parallel way to facilitate the comparison of pectin yield and the compositional and structural properties of the extracted pectin and residual cell wall material obtained.
34065932	2	2	theme	tomato	355:360	arg1	AIR					336:338	AIR	336:338	AIR	336:338	In this study, two approaches starting from the alcohol-insoluble residue (AIR) of processing tomato are performed in a parallel way to facilitate the comparison of pectin yield and the compositional and structural properties of the extracted pectin and residual cell wall material obtained.
34065932	2	2	theme	tomato	355:360	arg1	residue					327:333	the alcohol-insoluble residue	305:333	the alcohol-insoluble residue (AIR) of processing tomato	305:360	In this study, two approaches starting from the alcohol-insoluble residue (AIR) of processing tomato are performed in a parallel way to facilitate the comparison of pectin yield and the compositional and structural properties of the extracted pectin and residual cell wall material obtained.
34065932	0	3	theme	Wall	68:71	arg1	Material					73:80	Residual Cell Wall Material	54:80	Residual Cell Wall Material from Processing Tomato	54:103	The Structure and Composition of Extracted Pectin and Residual Cell Wall Material from Processing Tomato: The Role of a Stepwise Approach versus High-Pressure Homogenization-Facilitated Acid Extraction.
34065932	0	4	theme	Homogenization-Facilitated	159:184	arg1	Extraction					191:200	High-Pressure Homogenization-Facilitated Acid Extraction	145:200	High-Pressure Homogenization-Facilitated Acid Extraction	145:200	The Structure and Composition of Extracted Pectin and Residual Cell Wall Material from Processing Tomato: The Role of a Stepwise Approach versus High-Pressure Homogenization-Facilitated Acid Extraction.
34065932	2	5	theme	processing	344:353	arg1	tomato					355:360	processing tomato	344:360	processing tomato	344:360	In this study, two approaches starting from the alcohol-insoluble residue (AIR) of processing tomato are performed in a parallel way to facilitate the comparison of pectin yield and the compositional and structural properties of the extracted pectin and residual cell wall material obtained.
34065932	0	6	theme	Processing	87:96	arg1	Tomato					98:103	Processing Tomato	87:103	Processing Tomato	87:103	The Structure and Composition of Extracted Pectin and Residual Cell Wall Material from Processing Tomato: The Role of a Stepwise Approach versus High-Pressure Homogenization-Facilitated Acid Extraction.
34065932	5	7	theme	further	980:986	arg1	extraction					995:1004	further pectin extraction	980:1004	further pectin extraction	980:1004	In addition to these conventional solvent pectin extractions, the role of high-pressure homogenization (HPH) as a physically disruptive treatment to facilitate further pectin extraction from the partially pectin-depleted fraction obtained after acid extraction is addressed.
34065932	7	8	theme	molecular	1373:1381	arg1	mass					1383:1386	a higher molecular mass	1364:1386	a higher molecular mass	1364:1386	For both extraction approaches, pectin obtained further in the sequence was characterized by a higher molecular mass and a higher amount of rhamnogalacturonan I domains.
34065932	4	9	theme	single-step	755:765	arg1	pH					798:799	pH 1.6	798:803	pH 1.6	798:803	On the other hand, an industrially relevant single-step nitric acid pectin extraction (pH 1.6) is performed.
34065932	4	9	theme	single-step	755:765	arg1	extraction					786:795	an industrially relevant single-step nitric acid pectin extraction	730:795	an industrially relevant single-step nitric acid pectin extraction (pH 1.6)	730:804	On the other hand, an industrially relevant single-step nitric acid pectin extraction (pH 1.6) is performed.
34065932	2	10	theme	residual	515:522	arg1	wall					529:532	residual cell wall	515:532	residual cell wall	515:532	In this study, two approaches starting from the alcohol-insoluble residue (AIR) of processing tomato are performed in a parallel way to facilitate the comparison of pectin yield and the compositional and structural properties of the extracted pectin and residual cell wall material obtained.
34065932	0	11	theme	Extraction	191:200	arg1	Role					110:113	The Role	106:113	The Structure and Composition of Extracted Pectin and Residual Cell Wall Material from Processing Tomato: The Role of a Stepwise Approach versus High-Pressure Homogenization-Facilitated Acid Extraction.	0:201	The Structure and Composition of Extracted Pectin and Residual Cell Wall Material from Processing Tomato: The Role of a Stepwise Approach versus High-Pressure Homogenization-Facilitated Acid Extraction.
34065932	4	12	theme	relevant	746:753	arg1	pH					798:799	pH 1.6	798:803	pH 1.6	798:803	On the other hand, an industrially relevant single-step nitric acid pectin extraction (pH 1.6) is performed.
34065932	4	12	theme	relevant	746:753	arg1	extraction					786:795	an industrially relevant single-step nitric acid pectin extraction	730:795	an industrially relevant single-step nitric acid pectin extraction (pH 1.6)	730:804	On the other hand, an industrially relevant single-step nitric acid pectin extraction (pH 1.6) is performed.
34065932	7	13	theme	extraction	1280:1289	arg1	approaches					1291:1300	both extraction approaches	1275:1300	both extraction approaches	1275:1300	For both extraction approaches, pectin obtained further in the sequence was characterized by a higher molecular mass and a higher amount of rhamnogalacturonan I domains.
34065932	4	14	theme	acid	774:777	arg1	pH					798:799	pH 1.6	798:803	pH 1.6	798:803	On the other hand, an industrially relevant single-step nitric acid pectin extraction (pH 1.6) is performed.
34065932	4	14	theme	acid	774:777	arg1	extraction					786:795	an industrially relevant single-step nitric acid pectin extraction	730:795	an industrially relevant single-step nitric acid pectin extraction (pH 1.6)	730:804	On the other hand, an industrially relevant single-step nitric acid pectin extraction (pH 1.6) is performed.
34065932	5	15	theme	homogenization	908:921	arg1	role					886:889	the role	882:889	the role of high-pressure homogenization (HPH) as a physically disruptive treatment to facilitate further pectin extraction from the partially pectin-depleted fraction obtained after acid extraction	882:1079	In addition to these conventional solvent pectin extractions, the role of high-pressure homogenization (HPH) as a physically disruptive treatment to facilitate further pectin extraction from the partially pectin-depleted fraction obtained after acid extraction is addressed.
34065932	2	16	theme	pectin	504:509	arg1	material					534:541	the extracted pectin and residual cell wall material	490:541	the extracted pectin and residual cell wall material obtained	490:550	In this study, two approaches starting from the alcohol-insoluble residue (AIR) of processing tomato are performed in a parallel way to facilitate the comparison of pectin yield and the compositional and structural properties of the extracted pectin and residual cell wall material obtained.
34065932	2	17	theme	structural	465:474	arg1	properties					476:485	the compositional and structural properties	443:485	the compositional and structural properties of the extracted pectin and residual cell wall material obtained	443:550	In this study, two approaches starting from the alcohol-insoluble residue (AIR) of processing tomato are performed in a parallel way to facilitate the comparison of pectin yield and the compositional and structural properties of the extracted pectin and residual cell wall material obtained.
34065932	4	18	theme	nitric	767:772	arg1	pH					798:799	pH 1.6	798:803	pH 1.6	798:803	On the other hand, an industrially relevant single-step nitric acid pectin extraction (pH 1.6) is performed.
34065932	4	18	theme	nitric	767:772	arg1	extraction					786:795	an industrially relevant single-step nitric acid pectin extraction	730:795	an industrially relevant single-step nitric acid pectin extraction (pH 1.6)	730:804	On the other hand, an industrially relevant single-step nitric acid pectin extraction (pH 1.6) is performed.
34065932	2	19	theme	parallel	381:388	arg1	way					390:392	a parallel way	379:392	a parallel way	379:392	In this study, two approaches starting from the alcohol-insoluble residue (AIR) of processing tomato are performed in a parallel way to facilitate the comparison of pectin yield and the compositional and structural properties of the extracted pectin and residual cell wall material obtained.
34065932	6	20	theme	subsequent	1247:1256	arg1	extractions					1258:1268	the subsequent extractions	1243:1268	the subsequent extractions	1243:1268	The impact of HPH on the pectin cell wall polysaccharide interactions was shown as almost two thirds of the residual pectin were extractable during the subsequent extractions.
34065932	2	21	theme	extracted	494:502	arg1	material					534:541	the extracted pectin and residual cell wall material	490:541	the extracted pectin and residual cell wall material obtained	490:550	In this study, two approaches starting from the alcohol-insoluble residue (AIR) of processing tomato are performed in a parallel way to facilitate the comparison of pectin yield and the compositional and structural properties of the extracted pectin and residual cell wall material obtained.
34065932	8	22	theme	methods	1590:1596	arg1	fractions					1572:1580	the final unextractable fractions	1548:1580	the final unextractable fractions of both methods	1548:1596	The estimated hemicellulose and cellulose content increased from 56 mol% for the AIR to almost 90 mol% for the final unextractable fractions of both methods.
34065932	5	23	theme	solvent	854:860	arg1	extractions					869:879	these conventional solvent pectin extractions	835:879	these conventional solvent pectin extractions	835:879	In addition to these conventional solvent pectin extractions, the role of high-pressure homogenization (HPH) as a physically disruptive treatment to facilitate further pectin extraction from the partially pectin-depleted fraction obtained after acid extraction is addressed.
34065932	2	24	theme	yield	433:437	arg1	properties					476:485	the compositional and structural properties	443:485	the compositional and structural properties of the extracted pectin and residual cell wall material obtained	443:550	In this study, two approaches starting from the alcohol-insoluble residue (AIR) of processing tomato are performed in a parallel way to facilitate the comparison of pectin yield and the compositional and structural properties of the extracted pectin and residual cell wall material obtained.
34065932	2	24	theme	yield	433:437	arg1	comparison					412:421	the comparison	408:421	the comparison of pectin yield	408:437	In this study, two approaches starting from the alcohol-insoluble residue (AIR) of processing tomato are performed in a parallel way to facilitate the comparison of pectin yield and the compositional and structural properties of the extracted pectin and residual cell wall material obtained.
34065932	8	25	theme	mol	1509:1511	arg1	%					1512:1512	56 mol%	1506:1512	56 mol%	1506:1512	The estimated hemicellulose and cellulose content increased from 56 mol% for the AIR to almost 90 mol% for the final unextractable fractions of both methods.
34065932	2	26	theme	alcohol-insoluble	309:325	arg1	tomato					355:360	processing tomato	344:360	processing tomato	344:360	In this study, two approaches starting from the alcohol-insoluble residue (AIR) of processing tomato are performed in a parallel way to facilitate the comparison of pectin yield and the compositional and structural properties of the extracted pectin and residual cell wall material obtained.
34065932	2	26	theme	alcohol-insoluble	309:325	arg1	AIR					336:338	AIR	336:338	AIR	336:338	In this study, two approaches starting from the alcohol-insoluble residue (AIR) of processing tomato are performed in a parallel way to facilitate the comparison of pectin yield and the compositional and structural properties of the extracted pectin and residual cell wall material obtained.
34065932	2	26	theme	alcohol-insoluble	309:325	arg1	residue					327:333	the alcohol-insoluble residue	305:333	the alcohol-insoluble residue (AIR) of processing tomato	305:360	In this study, two approaches starting from the alcohol-insoluble residue (AIR) of processing tomato are performed in a parallel way to facilitate the comparison of pectin yield and the compositional and structural properties of the extracted pectin and residual cell wall material obtained.
34065932	5	27	theme	pectin	862:867	arg1	extractions					869:879	these conventional solvent pectin extractions	835:879	these conventional solvent pectin extractions	835:879	In addition to these conventional solvent pectin extractions, the role of high-pressure homogenization (HPH) as a physically disruptive treatment to facilitate further pectin extraction from the partially pectin-depleted fraction obtained after acid extraction is addressed.
34065932	2	28	theme	pectin	426:431	arg1	yield					433:437	pectin yield	426:437	pectin yield	426:437	In this study, two approaches starting from the alcohol-insoluble residue (AIR) of processing tomato are performed in a parallel way to facilitate the comparison of pectin yield and the compositional and structural properties of the extracted pectin and residual cell wall material obtained.
34065932	5	29	theme	pectin	988:993	arg1	extraction					995:1004	further pectin extraction	980:1004	further pectin extraction	980:1004	In addition to these conventional solvent pectin extractions, the role of high-pressure homogenization (HPH) as a physically disruptive treatment to facilitate further pectin extraction from the partially pectin-depleted fraction obtained after acid extraction is addressed.
34065932	6	30	theme	pectin	1212:1217	arg1	thirds					1189:1194	almost two thirds	1178:1194	almost two thirds of the residual pectin	1178:1217	The impact of HPH on the pectin cell wall polysaccharide interactions was shown as almost two thirds of the residual pectin were extractable during the subsequent extractions.
34065932	5	31	dep	extractions	869:879	arg1	addition					823:830	addition	823:830	addition	823:830	In addition to these conventional solvent pectin extractions, the role of high-pressure homogenization (HPH) as a physically disruptive treatment to facilitate further pectin extraction from the partially pectin-depleted fraction obtained after acid extraction is addressed.
34065932	7	32	theme	rhamnogalacturonan	1411:1428	arg1	domains					1432:1438	rhamnogalacturonan I domains	1411:1438	rhamnogalacturonan I domains	1411:1438	For both extraction approaches, pectin obtained further in the sequence was characterized by a higher molecular mass and a higher amount of rhamnogalacturonan I domains.
34065932	7	33	theme	I	1430:1430	arg1	domains					1432:1438	rhamnogalacturonan I domains	1411:1438	rhamnogalacturonan I domains	1411:1438	For both extraction approaches, pectin obtained further in the sequence was characterized by a higher molecular mass and a higher amount of rhamnogalacturonan I domains.
34065932	8	34	theme	hemicellulose	1455:1467	arg1	content					1483:1489	The estimated hemicellulose and cellulose content	1441:1489	The estimated hemicellulose and cellulose content	1441:1489	The estimated hemicellulose and cellulose content increased from 56 mol% for the AIR to almost 90 mol% for the final unextractable fractions of both methods.
34065932	6	35	theme	wall	1132:1135	arg1	interactions					1152:1163	the pectin cell wall polysaccharide interactions	1116:1163	the pectin cell wall polysaccharide interactions	1116:1163	The impact of HPH on the pectin cell wall polysaccharide interactions was shown as almost two thirds of the residual pectin were extractable during the subsequent extractions.
34065932	7	36	theme	higher	1366:1371	arg1	mass					1383:1386	a higher molecular mass	1364:1386	a higher molecular mass	1364:1386	For both extraction approaches, pectin obtained further in the sequence was characterized by a higher molecular mass and a higher amount of rhamnogalacturonan I domains.
34065932	0	37	theme	Extracted	33:41	arg1	Pectin					43:48	Extracted Pectin	33:48	Extracted Pectin	33:48	The Structure and Composition of Extracted Pectin and Residual Cell Wall Material from Processing Tomato: The Role of a Stepwise Approach versus High-Pressure Homogenization-Facilitated Acid Extraction.
34065932	6	38	theme	pectin	1120:1125	arg1	interactions					1152:1163	the pectin cell wall polysaccharide interactions	1116:1163	the pectin cell wall polysaccharide interactions	1116:1163	The impact of HPH on the pectin cell wall polysaccharide interactions was shown as almost two thirds of the residual pectin were extractable during the subsequent extractions.
34065932	2	39	theme	material	534:541	arg1	properties					476:485	the compositional and structural properties	443:485	the compositional and structural properties of the extracted pectin and residual cell wall material obtained	443:550	In this study, two approaches starting from the alcohol-insoluble residue (AIR) of processing tomato are performed in a parallel way to facilitate the comparison of pectin yield and the compositional and structural properties of the extracted pectin and residual cell wall material obtained.
34065932	2	39	theme	material	534:541	arg1	comparison					412:421	the comparison	408:421	the comparison of pectin yield	408:437	In this study, two approaches starting from the alcohol-insoluble residue (AIR) of processing tomato are performed in a parallel way to facilitate the comparison of pectin yield and the compositional and structural properties of the extracted pectin and residual cell wall material obtained.
34065932	8	40	theme	estimated	1445:1453	arg1	content					1483:1489	The estimated hemicellulose and cellulose content	1441:1489	The estimated hemicellulose and cellulose content	1441:1489	The estimated hemicellulose and cellulose content increased from 56 mol% for the AIR to almost 90 mol% for the final unextractable fractions of both methods.
34065932	7	41	theme	higher	1394:1399	arg1	domains					1432:1438	rhamnogalacturonan I domains	1411:1438	rhamnogalacturonan I domains	1411:1438	For both extraction approaches, pectin obtained further in the sequence was characterized by a higher molecular mass and a higher amount of rhamnogalacturonan I domains.
34065932	7	41	theme	higher	1394:1399	arg1	amount					1401:1406	a higher amount	1392:1406	a higher amount of rhamnogalacturonan I domains	1392:1438	For both extraction approaches, pectin obtained further in the sequence was characterized by a higher molecular mass and a higher amount of rhamnogalacturonan I domains.
34065932	8	42	theme	unextractable	1558:1570	arg1	fractions					1572:1580	the final unextractable fractions	1548:1580	the final unextractable fractions of both methods	1548:1596	The estimated hemicellulose and cellulose content increased from 56 mol% for the AIR to almost 90 mol% for the final unextractable fractions of both methods.
34065932	2	43	theme	wall	529:532	arg1	material					534:541	the extracted pectin and residual cell wall material	490:541	the extracted pectin and residual cell wall material obtained	490:550	In this study, two approaches starting from the alcohol-insoluble residue (AIR) of processing tomato are performed in a parallel way to facilitate the comparison of pectin yield and the compositional and structural properties of the extracted pectin and residual cell wall material obtained.
34065932	8	44	theme	cellulose	1473:1481	arg1	content					1483:1489	The estimated hemicellulose and cellulose content	1441:1489	The estimated hemicellulose and cellulose content	1441:1489	The estimated hemicellulose and cellulose content increased from 56 mol% for the AIR to almost 90 mol% for the final unextractable fractions of both methods.
34065932	1	45	theme	different	218:226	arg1	methods					246:252	different pectin extraction methods	218:252	different pectin extraction methods	218:252	In literature, different pectin extraction methods exist.
34065932	2	46	theme	compositional	447:459	arg1	properties					476:485	the compositional and structural properties	443:485	the compositional and structural properties of the extracted pectin and residual cell wall material obtained	443:550	In this study, two approaches starting from the alcohol-insoluble residue (AIR) of processing tomato are performed in a parallel way to facilitate the comparison of pectin yield and the compositional and structural properties of the extracted pectin and residual cell wall material obtained.
34065932	2	47	theme	cell	524:527	arg1	wall					529:532	residual cell wall	515:532	residual cell wall	515:532	In this study, two approaches starting from the alcohol-insoluble residue (AIR) of processing tomato are performed in a parallel way to facilitate the comparison of pectin yield and the compositional and structural properties of the extracted pectin and residual cell wall material obtained.
34065932	0	48	theme	Approach	129:136	arg1	Role					110:113	The Role	106:113	The Structure and Composition of Extracted Pectin and Residual Cell Wall Material from Processing Tomato: The Role of a Stepwise Approach versus High-Pressure Homogenization-Facilitated Acid Extraction.	0:201	The Structure and Composition of Extracted Pectin and Residual Cell Wall Material from Processing Tomato: The Role of a Stepwise Approach versus High-Pressure Homogenization-Facilitated Acid Extraction.
34065932	3	49	theme	chelating	616:624	arg1	agents					626:631	chelating agents	616:631	chelating agents	616:631	On the one hand, pectin is extracted stepwise using hot water, chelating agents and low-alkaline conditions targeting fractionation of the pectin population.
34065932	3	49	theme	chelating	616:624	arg1	water					609:613	hot water	605:613	hot water	605:613	On the one hand, pectin is extracted stepwise using hot water, chelating agents and low-alkaline conditions targeting fractionation of the pectin population.
34065932	5	50	theme	disruptive	945:954	arg1	treatment					956:964	a physically disruptive treatment	932:964	a physically disruptive treatment to facilitate further pectin extraction from the partially pectin-depleted fraction obtained after acid extraction	932:1079	In addition to these conventional solvent pectin extractions, the role of high-pressure homogenization (HPH) as a physically disruptive treatment to facilitate further pectin extraction from the partially pectin-depleted fraction obtained after acid extraction is addressed.
34065932	6	51	theme	cell	1127:1130	arg1	interactions					1152:1163	the pectin cell wall polysaccharide interactions	1116:1163	the pectin cell wall polysaccharide interactions	1116:1163	The impact of HPH on the pectin cell wall polysaccharide interactions was shown as almost two thirds of the residual pectin were extractable during the subsequent extractions.
34065932	0	52	theme	Pectin	43:48	arg1	Material					73:80	Residual Cell Wall Material	54:80	Residual Cell Wall Material from Processing Tomato	54:103	The Structure and Composition of Extracted Pectin and Residual Cell Wall Material from Processing Tomato: The Role of a Stepwise Approach versus High-Pressure Homogenization-Facilitated Acid Extraction.
34065932	0	52	theme	Pectin	43:48	arg1	Structure					4:12	Structure	4:12	Structure	4:12	The Structure and Composition of Extracted Pectin and Residual Cell Wall Material from Processing Tomato: The Role of a Stepwise Approach versus High-Pressure Homogenization-Facilitated Acid Extraction.
34065932	0	52	theme	Pectin	43:48	arg1	Composition					18:28	Composition	18:28	Composition	18:28	The Structure and Composition of Extracted Pectin and Residual Cell Wall Material from Processing Tomato: The Role of a Stepwise Approach versus High-Pressure Homogenization-Facilitated Acid Extraction.
34065932	0	53	theme	Stepwise	120:127	arg1	Approach					129:136	a Stepwise Approach	118:136	a Stepwise Approach	118:136	The Structure and Composition of Extracted Pectin and Residual Cell Wall Material from Processing Tomato: The Role of a Stepwise Approach versus High-Pressure Homogenization-Facilitated Acid Extraction.
34065932	5	54	theme	pectin-depleted	1025:1039	arg1	fraction					1041:1048	the partially pectin-depleted fraction	1011:1048	the partially pectin-depleted fraction obtained after acid extraction	1011:1079	In addition to these conventional solvent pectin extractions, the role of high-pressure homogenization (HPH) as a physically disruptive treatment to facilitate further pectin extraction from the partially pectin-depleted fraction obtained after acid extraction is addressed.
34065932	5	55	theme	high-pressure	894:906	arg1	HPH					924:926	HPH	924:926	HPH	924:926	In addition to these conventional solvent pectin extractions, the role of high-pressure homogenization (HPH) as a physically disruptive treatment to facilitate further pectin extraction from the partially pectin-depleted fraction obtained after acid extraction is addressed.
34065932	5	55	theme	high-pressure	894:906	arg1	homogenization					908:921	high-pressure homogenization	894:921	high-pressure homogenization (HPH)	894:927	In addition to these conventional solvent pectin extractions, the role of high-pressure homogenization (HPH) as a physically disruptive treatment to facilitate further pectin extraction from the partially pectin-depleted fraction obtained after acid extraction is addressed.
34065932	0	56	theme	High-Pressure	145:157	arg1	Extraction					191:200	High-Pressure Homogenization-Facilitated Acid Extraction	145:200	High-Pressure Homogenization-Facilitated Acid Extraction	145:200	The Structure and Composition of Extracted Pectin and Residual Cell Wall Material from Processing Tomato: The Role of a Stepwise Approach versus High-Pressure Homogenization-Facilitated Acid Extraction.
34065932	0	57	from	Tomato	98:103	arg1	Material					73:80	Residual Cell Wall Material	54:80	Residual Cell Wall Material from Processing Tomato	54:103	The Structure and Composition of Extracted Pectin and Residual Cell Wall Material from Processing Tomato: The Role of a Stepwise Approach versus High-Pressure Homogenization-Facilitated Acid Extraction.
34065932	0	57	from	Tomato	98:103	arg1	Structure					4:12	Structure	4:12	Structure	4:12	The Structure and Composition of Extracted Pectin and Residual Cell Wall Material from Processing Tomato: The Role of a Stepwise Approach versus High-Pressure Homogenization-Facilitated Acid Extraction.
34065932	0	57	from	Tomato	98:103	arg1	Composition					18:28	Composition	18:28	Composition	18:28	The Structure and Composition of Extracted Pectin and Residual Cell Wall Material from Processing Tomato: The Role of a Stepwise Approach versus High-Pressure Homogenization-Facilitated Acid Extraction.
34065932	3	58	theme	hot	605:607	arg1	agents					626:631	chelating agents	616:631	chelating agents	616:631	On the one hand, pectin is extracted stepwise using hot water, chelating agents and low-alkaline conditions targeting fractionation of the pectin population.
34065932	3	58	theme	hot	605:607	arg1	conditions					650:659	low-alkaline conditions	637:659	low-alkaline conditions	637:659	On the one hand, pectin is extracted stepwise using hot water, chelating agents and low-alkaline conditions targeting fractionation of the pectin population.
34065932	3	58	theme	hot	605:607	arg1	water					609:613	hot water	605:613	hot water	605:613	On the one hand, pectin is extracted stepwise using hot water, chelating agents and low-alkaline conditions targeting fractionation of the pectin population.
34065932	4	59	theme	other	718:722	arg1	hand					724:727	the other hand	714:727	the other hand	714:727	On the other hand, an industrially relevant single-step nitric acid pectin extraction (pH 1.6) is performed.
34065932	6	60	theme	polysaccharide	1137:1150	arg1	interactions					1152:1163	the pectin cell wall polysaccharide interactions	1116:1163	the pectin cell wall polysaccharide interactions	1116:1163	The impact of HPH on the pectin cell wall polysaccharide interactions was shown as almost two thirds of the residual pectin were extractable during the subsequent extractions.
34065932	1	61	theme	pectin	228:233	arg1	methods					246:252	different pectin extraction methods	218:252	different pectin extraction methods	218:252	In literature, different pectin extraction methods exist.
34065932	7	62	theme	domains	1432:1438	arg1	mass					1383:1386	a higher molecular mass	1364:1386	a higher molecular mass	1364:1386	For both extraction approaches, pectin obtained further in the sequence was characterized by a higher molecular mass and a higher amount of rhamnogalacturonan I domains.
34065932	7	62	theme	domains	1432:1438	arg1	amount					1401:1406	a higher amount	1392:1406	a higher amount of rhamnogalacturonan I domains	1392:1438	For both extraction approaches, pectin obtained further in the sequence was characterized by a higher molecular mass and a higher amount of rhamnogalacturonan I domains.
34065932	7	62	theme	domains	1432:1438	arg1	domains					1432:1438	rhamnogalacturonan I domains	1411:1438	rhamnogalacturonan I domains	1411:1438	For both extraction approaches, pectin obtained further in the sequence was characterized by a higher molecular mass and a higher amount of rhamnogalacturonan I domains.
34065932	3	63	theme	low-alkaline	637:648	arg1	conditions					650:659	low-alkaline conditions	637:659	low-alkaline conditions	637:659	On the one hand, pectin is extracted stepwise using hot water, chelating agents and low-alkaline conditions targeting fractionation of the pectin population.
34065932	3	63	theme	low-alkaline	637:648	arg1	water					609:613	hot water	605:613	hot water	605:613	On the one hand, pectin is extracted stepwise using hot water, chelating agents and low-alkaline conditions targeting fractionation of the pectin population.
34065932	4	64	theme	pectin	779:784	arg1	pH					798:799	pH 1.6	798:803	pH 1.6	798:803	On the other hand, an industrially relevant single-step nitric acid pectin extraction (pH 1.6) is performed.
34065932	4	64	theme	pectin	779:784	arg1	extraction					786:795	an industrially relevant single-step nitric acid pectin extraction	730:795	an industrially relevant single-step nitric acid pectin extraction (pH 1.6)	730:804	On the other hand, an industrially relevant single-step nitric acid pectin extraction (pH 1.6) is performed.
34065932	6	65	from	impact	1099:1104	arg1	interactions					1152:1163	the pectin cell wall polysaccharide interactions	1116:1163	the pectin cell wall polysaccharide interactions	1116:1163	The impact of HPH on the pectin cell wall polysaccharide interactions was shown as almost two thirds of the residual pectin were extractable during the subsequent extractions.
34065932	1	66	theme	extraction	235:244	arg1	methods					246:252	different pectin extraction methods	218:252	different pectin extraction methods	218:252	In literature, different pectin extraction methods exist.
34065932	0	67	theme	Cell	63:66	arg1	Material					73:80	Residual Cell Wall Material	54:80	Residual Cell Wall Material from Processing Tomato	54:103	The Structure and Composition of Extracted Pectin and Residual Cell Wall Material from Processing Tomato: The Role of a Stepwise Approach versus High-Pressure Homogenization-Facilitated Acid Extraction.
34065932	5	68	theme	acid	1065:1068	arg1	extraction					1070:1079	acid extraction	1065:1079	acid extraction	1065:1079	In addition to these conventional solvent pectin extractions, the role of high-pressure homogenization (HPH) as a physically disruptive treatment to facilitate further pectin extraction from the partially pectin-depleted fraction obtained after acid extraction is addressed.
34065932	0	69	dep	Structure	4:12	arg1	The					0:2	The	0:2	The	0:2	The Structure and Composition of Extracted Pectin and Residual Cell Wall Material from Processing Tomato: The Role of a Stepwise Approach versus High-Pressure Homogenization-Facilitated Acid Extraction.
34065932	0	69	dep	Structure	4:12	arg1	Role					110:113	The Role	106:113	The Structure and Composition of Extracted Pectin and Residual Cell Wall Material from Processing Tomato: The Role of a Stepwise Approach versus High-Pressure Homogenization-Facilitated Acid Extraction.	0:201	The Structure and Composition of Extracted Pectin and Residual Cell Wall Material from Processing Tomato: The Role of a Stepwise Approach versus High-Pressure Homogenization-Facilitated Acid Extraction.
34065932	8	70	theme	mol	1539:1541	arg1	%					1542:1542	almost 90 mol%	1529:1542	almost 90 mol%	1529:1542	The estimated hemicellulose and cellulose content increased from 56 mol% for the AIR to almost 90 mol% for the final unextractable fractions of both methods.
34065932	6	71	theme	HPH	1109:1111	arg1	impact					1099:1104	The impact	1095:1104	The impact of HPH on the pectin cell wall polysaccharide interactions	1095:1163	The impact of HPH on the pectin cell wall polysaccharide interactions was shown as almost two thirds of the residual pectin were extractable during the subsequent extractions.
34065932	5	72	theme	conventional	841:852	arg1	extractions					869:879	these conventional solvent pectin extractions	835:879	these conventional solvent pectin extractions	835:879	In addition to these conventional solvent pectin extractions, the role of high-pressure homogenization (HPH) as a physically disruptive treatment to facilitate further pectin extraction from the partially pectin-depleted fraction obtained after acid extraction is addressed.
34065932	0	73	theme	Residual	54:61	arg1	Material					73:80	Residual Cell Wall Material	54:80	Residual Cell Wall Material from Processing Tomato	54:103	The Structure and Composition of Extracted Pectin and Residual Cell Wall Material from Processing Tomato: The Role of a Stepwise Approach versus High-Pressure Homogenization-Facilitated Acid Extraction.
34065932	3	74	theme	pectin	692:697	arg1	population					699:708	the pectin population	688:708	the pectin population	688:708	On the one hand, pectin is extracted stepwise using hot water, chelating agents and low-alkaline conditions targeting fractionation of the pectin population.
34065932	6	75	theme	residual	1203:1210	arg1	pectin					1212:1217	the residual pectin	1199:1217	the residual pectin	1199:1217	The impact of HPH on the pectin cell wall polysaccharide interactions was shown as almost two thirds of the residual pectin were extractable during the subsequent extractions.
34065932	8	76	theme	final	1552:1556	arg1	fractions					1572:1580	the final unextractable fractions	1548:1580	the final unextractable fractions of both methods	1548:1596	The estimated hemicellulose and cellulose content increased from 56 mol% for the AIR to almost 90 mol% for the final unextractable fractions of both methods.
35573890	5	0	theme	mass	884:887	arg1	approach					902:909	a top-down mass spectrometry approach	873:909	a top-down mass spectrometry approach	873:909	First, the differences between CIs were explored at the intact protein level using a top-down mass spectrometry approach; this showed differences in glycoforms and deamidation status.
35573890	3	1	theme	posttranslational	507:523	arg1	modifications					525:537	posttranslational modifications	507:537	posttranslational modifications	507:537	We intended to quantitatively characterize the posttranslational modification status of CIs of antibody drugs and explore the impact of posttranslational modifications on charge heterogeneity.
35573890	12	2	theme	analysis	1788:1795	arg1	control					1759:1765	the quality control	1747:1765	the quality control of biopharmaceutical analysis	1747:1795	This study demonstrated the importance of the comprehensive analyses of antibody CIs and provides a reference method for the quality control of biopharmaceutical analysis.
35573890	4	3	theme	strong	607:612	arg1	chromatography					630:643	strong cation exchange chromatography	607:643	strong cation exchange chromatography	607:643	The CIs of antibodies were fractionated by strong cation exchange chromatography and verified by capillary isoelectric focusing-whole column imaging detection, followed by stepwise structural characterization at three levels.
35573890	0	4	theme	antibody	70:77	arg1	isomers					55:61	the charge isomers	44:61	the charge isomers of IgG antibody	44:77	Global characterization of modifications to the charge isomers of IgG antibody.
35573890	9	5	theme	in-depth	1243:1250	arg1	analysis					1252:1259	an in-depth analysis	1240:1259	an in-depth analysis of glycan variants of CIs	1240:1285	Finally, an in-depth analysis of glycan variants of CIs was performed through the detection of enriched glycopeptides.
35573890	3	6	theme	modifications	525:537	arg1	impact					497:502	the impact	493:502	the impact of posttranslational modifications on charge heterogeneity	493:561	We intended to quantitatively characterize the posttranslational modification status of CIs of antibody drugs and explore the impact of posttranslational modifications on charge heterogeneity.
35573890	2	7	theme	charge	305:310	arg1	CIs					321:323	CIs	321:323	CIs	321:323	The comprehensive mapping of antibody modifications and different charge isomers (CIs) is of utmost importance, but is challenging.
35573890	2	7	theme	charge	305:310	arg1	isomers					312:318	different charge isomers	295:318	different charge isomers (CIs)	295:324	The comprehensive mapping of antibody modifications and different charge isomers (CIs) is of utmost importance, but is challenging.
35573890	1	8	theme	antibody	115:122	arg1	products					124:131	antibody products	115:131	antibody products	115:131	Posttranslational modifications of antibody products affect their stability, charge distribution, and drug activity and are thus a critical quality attribute.
35573890	4	9	theme	exchange	621:628	arg1	chromatography					630:643	strong cation exchange chromatography	607:643	strong cation exchange chromatography	607:643	The CIs of antibodies were fractionated by strong cation exchange chromatography and verified by capillary isoelectric focusing-whole column imaging detection, followed by stepwise structural characterization at three levels.
35573890	11	10	theme	charge	1521:1526	arg1	heterogeneity					1528:1540	charge heterogeneity	1521:1540	charge heterogeneity	1521:1540	The results revealed that sialic acid modification is a critical factor accounting for charge heterogeneity, which is otherwise missed in peptide mapping and intact molecular weight analyses.
35573890	2	11	theme	different	295:303	arg1	CIs					321:323	CIs	321:323	CIs	321:323	The comprehensive mapping of antibody modifications and different charge isomers (CIs) is of utmost importance, but is challenging.
35573890	2	11	theme	different	295:303	arg1	isomers					312:318	different charge isomers	295:318	different charge isomers (CIs)	295:324	The comprehensive mapping of antibody modifications and different charge isomers (CIs) is of utmost importance, but is challenging.
35573890	12	12	theme	biopharmaceutical	1770:1786	arg1	analysis					1788:1795	biopharmaceutical analysis	1770:1795	biopharmaceutical analysis	1770:1795	This study demonstrated the importance of the comprehensive analyses of antibody CIs and provides a reference method for the quality control of biopharmaceutical analysis.
35573890	4	13	theme	cation	614:619	arg1	chromatography					630:643	strong cation exchange chromatography	607:643	strong cation exchange chromatography	607:643	The CIs of antibodies were fractionated by strong cation exchange chromatography and verified by capillary isoelectric focusing-whole column imaging detection, followed by stepwise structural characterization at three levels.
35573890	4	14	theme	structural	745:754	arg1	characterization					756:771	stepwise structural characterization	736:771	stepwise structural characterization at three levels	736:787	The CIs of antibodies were fractionated by strong cation exchange chromatography and verified by capillary isoelectric focusing-whole column imaging detection, followed by stepwise structural characterization at three levels.
35573890	5	15	theme	spectrometry	889:900	arg1	approach					902:909	a top-down mass spectrometry approach	873:909	a top-down mass spectrometry approach	873:909	First, the differences between CIs were explored at the intact protein level using a top-down mass spectrometry approach; this showed differences in glycoforms and deamidation status.
35573890	10	16	theme	quantitative	1366:1377	arg1	analyses					1379:1386	Qualitative and quantitative analyses	1350:1386	Qualitative and quantitative analyses	1350:1386	Qualitative and quantitative analyses demonstrated the dynamics of 24 N-glycoforms.
35573890	5	17	theme	protein	853:859	arg1	level					861:865	the intact protein level	842:865	the intact protein level	842:865	First, the differences between CIs were explored at the intact protein level using a top-down mass spectrometry approach; this showed differences in glycoforms and deamidation status.
35573890	9	18	theme	variants	1271:1278	arg1	analysis					1252:1259	an in-depth analysis	1240:1259	an in-depth analysis of glycan variants of CIs	1240:1285	Finally, an in-depth analysis of glycan variants of CIs was performed through the detection of enriched glycopeptides.
35573890	2	19	theme	modifications	277:289	arg1	mapping					257:263	The comprehensive mapping	239:263	The comprehensive mapping of antibody modifications and different charge isomers (CIs)	239:324	The comprehensive mapping of antibody modifications and different charge isomers (CIs) is of utmost importance, but is challenging.
35573890	1	20	theme	drug	182:185	arg1	activity					187:194	drug activity	182:194	drug activity	182:194	Posttranslational modifications of antibody products affect their stability, charge distribution, and drug activity and are thus a critical quality attribute.
35573890	12	21	theme	reference	1726:1734	arg1	method					1736:1741	a reference method	1724:1741	a reference method for the quality control of biopharmaceutical analysis	1724:1795	This study demonstrated the importance of the comprehensive analyses of antibody CIs and provides a reference method for the quality control of biopharmaceutical analysis.
35573890	8	22	theme	peptide	1214:1220	arg1	mapping					1222:1228	peptide mapping	1214:1228	peptide mapping	1214:1228	In total, 10 N-glycoforms were detected by peptide mapping.
35573890	6	23	theme	oxidation	1028:1036	arg1	modifications					1011:1023	common modifications	1004:1023	common modifications of oxidation, deamidation, and glycosylation	1004:1068	Second, at the peptide level, common modifications of oxidation, deamidation, and glycosylation were identified.
35573890	1	24	theme	Posttranslational	80:96	arg1	modifications					98:110	Posttranslational modifications	80:110	Posttranslational modifications of antibody products	80:131	Posttranslational modifications of antibody products affect their stability, charge distribution, and drug activity and are thus a critical quality attribute.
35573890	10	25	theme	Qualitative	1350:1360	arg1	analyses					1379:1386	Qualitative and quantitative analyses	1350:1386	Qualitative and quantitative analyses	1350:1386	Qualitative and quantitative analyses demonstrated the dynamics of 24 N-glycoforms.
35573890	3	26	theme	modification	436:447	arg1	status					449:454	the posttranslational modification status	414:454	the posttranslational modification status of CIs of antibody drugs	414:479	We intended to quantitatively characterize the posttranslational modification status of CIs of antibody drugs and explore the impact of posttranslational modifications on charge heterogeneity.
35573890	0	27	theme	Global	0:5	arg1	characterization					7:22	Global characterization	0:22	Global characterization of modifications to the charge isomers of IgG antibody.	0:78	Global characterization of modifications to the charge isomers of IgG antibody.
35573890	4	28	theme	antibodies	575:584	arg1	CIs					568:570	The CIs	564:570	The CIs of antibodies	564:584	The CIs of antibodies were fractionated by strong cation exchange chromatography and verified by capillary isoelectric focusing-whole column imaging detection, followed by stepwise structural characterization at three levels.
35573890	3	29	from	impact	497:502	arg1	heterogeneity					549:561	charge heterogeneity	542:561	charge heterogeneity	542:561	We intended to quantitatively characterize the posttranslational modification status of CIs of antibody drugs and explore the impact of posttranslational modifications on charge heterogeneity.
35573890	0	30	theme	modifications	27:39	arg1	characterization					7:22	Global characterization	0:22	Global characterization of modifications to the charge isomers of IgG antibody.	0:78	Global characterization of modifications to the charge isomers of IgG antibody.
35573890	9	31	theme	CIs	1283:1285	arg1	variants					1271:1278	glycan variants	1264:1278	glycan variants of CIs	1264:1285	Finally, an in-depth analysis of glycan variants of CIs was performed through the detection of enriched glycopeptides.
35573890	7	32	theme	glycoform	1137:1145	arg1	distribution					1147:1158	nonuniform deamidation and glycoform distribution	1110:1158	nonuniform deamidation and glycoform distribution among CIs	1110:1168	Peptide mapping showed nonuniform deamidation and glycoform distribution among CIs.
35573890	9	33	theme	glycan	1264:1269	arg1	variants					1271:1278	glycan variants	1264:1278	glycan variants of CIs	1264:1285	Finally, an in-depth analysis of glycan variants of CIs was performed through the detection of enriched glycopeptides.
35573890	5	34	dep	glycoforms	939:948	arg1	status					966:971	status	966:971	status	966:971	First, the differences between CIs were explored at the intact protein level using a top-down mass spectrometry approach; this showed differences in glycoforms and deamidation status.
35573890	11	35	theme	peptide	1572:1578	arg1	mapping					1580:1586	peptide mapping	1572:1586	peptide mapping	1572:1586	The results revealed that sialic acid modification is a critical factor accounting for charge heterogeneity, which is otherwise missed in peptide mapping and intact molecular weight analyses.
35573890	3	36	theme	drugs	475:479	arg1	status					449:454	the posttranslational modification status	414:454	the posttranslational modification status of CIs of antibody drugs	414:479	We intended to quantitatively characterize the posttranslational modification status of CIs of antibody drugs and explore the impact of posttranslational modifications on charge heterogeneity.
35573890	5	37	theme	top-down	875:882	arg1	approach					902:909	a top-down mass spectrometry approach	873:909	a top-down mass spectrometry approach	873:909	First, the differences between CIs were explored at the intact protein level using a top-down mass spectrometry approach; this showed differences in glycoforms and deamidation status.
35573890	11	38	theme	molecular	1599:1607	arg1	weight					1609:1614	intact molecular weight	1592:1614	intact molecular weight	1592:1614	The results revealed that sialic acid modification is a critical factor accounting for charge heterogeneity, which is otherwise missed in peptide mapping and intact molecular weight analyses.
35573890	2	39	theme	isomers	312:318	arg1	mapping					257:263	The comprehensive mapping	239:263	The comprehensive mapping of antibody modifications and different charge isomers (CIs)	239:324	The comprehensive mapping of antibody modifications and different charge isomers (CIs) is of utmost importance, but is challenging.
35573890	4	40	theme	capillary	661:669	arg1	column					698:703	capillary isoelectric focusing-whole column	661:703	capillary isoelectric focusing-whole column imaging detection	661:721	The CIs of antibodies were fractionated by strong cation exchange chromatography and verified by capillary isoelectric focusing-whole column imaging detection, followed by stepwise structural characterization at three levels.
35573890	11	41	theme	sialic	1460:1465	arg1	modification					1472:1483	sialic acid modification	1460:1483	sialic acid modification	1460:1483	The results revealed that sialic acid modification is a critical factor accounting for charge heterogeneity, which is otherwise missed in peptide mapping and intact molecular weight analyses.
35573890	11	41	theme	sialic	1460:1465	arg1	factor					1499:1504	a critical factor	1488:1504	a critical factor	1488:1504	The results revealed that sialic acid modification is a critical factor accounting for charge heterogeneity, which is otherwise missed in peptide mapping and intact molecular weight analyses.
35573890	6	42	theme	common	1004:1009	arg1	modifications					1011:1023	common modifications	1004:1023	common modifications of oxidation, deamidation, and glycosylation	1004:1068	Second, at the peptide level, common modifications of oxidation, deamidation, and glycosylation were identified.
35573890	7	43	theme	deamidation	1121:1131	arg1	distribution					1147:1158	nonuniform deamidation and glycoform distribution	1110:1158	nonuniform deamidation and glycoform distribution among CIs	1110:1168	Peptide mapping showed nonuniform deamidation and glycoform distribution among CIs.
35573890	4	44	theme	focusing-whole	683:696	arg1	column					698:703	capillary isoelectric focusing-whole column	661:703	capillary isoelectric focusing-whole column imaging detection	661:721	The CIs of antibodies were fractionated by strong cation exchange chromatography and verified by capillary isoelectric focusing-whole column imaging detection, followed by stepwise structural characterization at three levels.
35573890	12	45	theme	quality	1751:1757	arg1	control					1759:1765	the quality control	1747:1765	the quality control of biopharmaceutical analysis	1747:1795	This study demonstrated the importance of the comprehensive analyses of antibody CIs and provides a reference method for the quality control of biopharmaceutical analysis.
35573890	3	46	theme	posttranslational	418:434	arg1	modification					436:447	posttranslational modification	418:447	the posttranslational modification status of CIs of antibody drugs	414:479	We intended to quantitatively characterize the posttranslational modification status of CIs of antibody drugs and explore the impact of posttranslational modifications on charge heterogeneity.
35573890	11	47	dep	mapping	1580:1586	arg1	analyses					1616:1623	analyses	1616:1623	analyses	1616:1623	The results revealed that sialic acid modification is a critical factor accounting for charge heterogeneity, which is otherwise missed in peptide mapping and intact molecular weight analyses.
35573890	11	48	theme	acid	1467:1470	arg1	modification					1472:1483	sialic acid modification	1460:1483	sialic acid modification	1460:1483	The results revealed that sialic acid modification is a critical factor accounting for charge heterogeneity, which is otherwise missed in peptide mapping and intact molecular weight analyses.
35573890	11	48	theme	acid	1467:1470	arg1	factor					1499:1504	a critical factor	1488:1504	a critical factor	1488:1504	The results revealed that sialic acid modification is a critical factor accounting for charge heterogeneity, which is otherwise missed in peptide mapping and intact molecular weight analyses.
35573890	12	49	theme	comprehensive	1672:1684	arg1	analyses					1686:1693	the comprehensive analyses	1668:1693	the comprehensive analyses of antibody CIs	1668:1709	This study demonstrated the importance of the comprehensive analyses of antibody CIs and provides a reference method for the quality control of biopharmaceutical analysis.
35573890	1	50	theme	critical	211:218	arg1	attribute					228:236	a critical quality attribute	209:236	a critical quality attribute	209:236	Posttranslational modifications of antibody products affect their stability, charge distribution, and drug activity and are thus a critical quality attribute.
35573890	5	51	theme	intact	846:851	arg1	level					861:865	the intact protein level	842:865	the intact protein level	842:865	First, the differences between CIs were explored at the intact protein level using a top-down mass spectrometry approach; this showed differences in glycoforms and deamidation status.
35573890	9	52	gly	glycopeptides	1335:1347	arg2	glycopeptides					1335:1347	enriched glycopeptides	1326:1347	enriched glycopeptides	1326:1347	Finally, an in-depth analysis of glycan variants of CIs was performed through the detection of enriched glycopeptides.
35573890	11	53	theme	critical	1490:1497	arg1	modification					1472:1483	sialic acid modification	1460:1483	sialic acid modification	1460:1483	The results revealed that sialic acid modification is a critical factor accounting for charge heterogeneity, which is otherwise missed in peptide mapping and intact molecular weight analyses.
35573890	11	53	theme	critical	1490:1497	arg1	factor					1499:1504	a critical factor	1488:1504	a critical factor	1488:1504	The results revealed that sialic acid modification is a critical factor accounting for charge heterogeneity, which is otherwise missed in peptide mapping and intact molecular weight analyses.
35573890	1	54	theme	products	124:131	arg1	modifications					98:110	Posttranslational modifications	80:110	Posttranslational modifications of antibody products	80:131	Posttranslational modifications of antibody products affect their stability, charge distribution, and drug activity and are thus a critical quality attribute.
35573890	0	55	theme	charge	48:53	arg1	isomers					55:61	the charge isomers	44:61	the charge isomers of IgG antibody	44:77	Global characterization of modifications to the charge isomers of IgG antibody.
35573890	6	56	theme	peptide	989:995	arg1	level					997:1001	the peptide level	985:1001	the peptide level	985:1001	Second, at the peptide level, common modifications of oxidation, deamidation, and glycosylation were identified.
35573890	7	57	theme	nonuniform	1110:1119	arg1	distribution					1147:1158	nonuniform deamidation and glycoform distribution	1110:1158	nonuniform deamidation and glycoform distribution among CIs	1110:1168	Peptide mapping showed nonuniform deamidation and glycoform distribution among CIs.
35573890	12	58	theme	antibody	1698:1705	arg1	CIs					1707:1709	antibody CIs	1698:1709	antibody CIs	1698:1709	This study demonstrated the importance of the comprehensive analyses of antibody CIs and provides a reference method for the quality control of biopharmaceutical analysis.
35573890	2	59	theme	utmost	332:337	arg1	importance					339:348	utmost importance	332:348	utmost importance	332:348	The comprehensive mapping of antibody modifications and different charge isomers (CIs) is of utmost importance, but is challenging.
35573890	11	60	theme	intact	1592:1597	arg1	weight					1609:1614	intact molecular weight	1592:1614	intact molecular weight	1592:1614	The results revealed that sialic acid modification is a critical factor accounting for charge heterogeneity, which is otherwise missed in peptide mapping and intact molecular weight analyses.
35573890	12	61	theme	CIs	1707:1709	arg1	analyses					1686:1693	the comprehensive analyses	1668:1693	the comprehensive analyses of antibody CIs	1668:1709	This study demonstrated the importance of the comprehensive analyses of antibody CIs and provides a reference method for the quality control of biopharmaceutical analysis.
35573890	7	62	theme	Peptide	1087:1093	arg1	mapping					1095:1101	Peptide mapping	1087:1101	Peptide mapping	1087:1101	Peptide mapping showed nonuniform deamidation and glycoform distribution among CIs.
35573890	9	63	theme	enriched	1326:1333	arg1	glycopeptides					1335:1347	enriched glycopeptides	1326:1347	enriched glycopeptides	1326:1347	Finally, an in-depth analysis of glycan variants of CIs was performed through the detection of enriched glycopeptides.
35573890	2	64	theme	antibody	268:275	arg1	modifications					277:289	antibody modifications	268:289	antibody modifications	268:289	The comprehensive mapping of antibody modifications and different charge isomers (CIs) is of utmost importance, but is challenging.
35573890	10	65	theme	24 N-glycoforms	1417:1431	arg1	dynamics					1405:1412	the dynamics	1401:1412	the dynamics of 24 N-glycoforms	1401:1431	Qualitative and quantitative analyses demonstrated the dynamics of 24 N-glycoforms.
35573890	4	66	from	levels	782:787	arg1	characterization					756:771	stepwise structural characterization	736:771	stepwise structural characterization at three levels	736:787	The CIs of antibodies were fractionated by strong cation exchange chromatography and verified by capillary isoelectric focusing-whole column imaging detection, followed by stepwise structural characterization at three levels.
35573890	1	67	theme	quality	220:226	arg1	attribute					228:236	a critical quality attribute	209:236	a critical quality attribute	209:236	Posttranslational modifications of antibody products affect their stability, charge distribution, and drug activity and are thus a critical quality attribute.
35573890	9	68	theme	glycopeptides	1335:1347	arg1	detection					1313:1321	the detection	1309:1321	the detection of enriched glycopeptides	1309:1347	Finally, an in-depth analysis of glycan variants of CIs was performed through the detection of enriched glycopeptides.
35573890	8	69	located	detected	1202:1209	arg2	10 N-glycoforms					1181:1195	10 N-glycoforms	1181:1195	10 N-glycoforms	1181:1195	In total, 10 N-glycoforms were detected by peptide mapping.
35573890	8	69	located	detected	1202:1209	arg1	total					1174:1178	total	1174:1178	total	1174:1178	In total, 10 N-glycoforms were detected by peptide mapping.
35573890	5	70	from	differences	924:934	arg1	deamidation					954:964	deamidation	954:964	deamidation	954:964	First, the differences between CIs were explored at the intact protein level using a top-down mass spectrometry approach; this showed differences in glycoforms and deamidation status.
35573890	5	70	from	differences	924:934	arg1	glycoforms					939:948	glycoforms	939:948	glycoforms	939:948	First, the differences between CIs were explored at the intact protein level using a top-down mass spectrometry approach; this showed differences in glycoforms and deamidation status.
35573890	1	71	theme	charge	157:162	arg1	distribution					164:175	charge distribution	157:175	charge distribution	157:175	Posttranslational modifications of antibody products affect their stability, charge distribution, and drug activity and are thus a critical quality attribute.
35573890	0	72	theme	IgG	66:68	arg1	antibody					70:77	IgG antibody	66:77	IgG antibody	66:77	Global characterization of modifications to the charge isomers of IgG antibody.
35573890	3	73	theme	antibody	466:473	arg1	drugs					475:479	antibody drugs	466:479	antibody drugs	466:479	We intended to quantitatively characterize the posttranslational modification status of CIs of antibody drugs and explore the impact of posttranslational modifications on charge heterogeneity.
35573890	4	74	theme	isoelectric	671:681	arg1	column					698:703	capillary isoelectric focusing-whole column	661:703	capillary isoelectric focusing-whole column imaging detection	661:721	The CIs of antibodies were fractionated by strong cation exchange chromatography and verified by capillary isoelectric focusing-whole column imaging detection, followed by stepwise structural characterization at three levels.
35573890	4	75	theme	stepwise	736:743	arg1	characterization					756:771	stepwise structural characterization	736:771	stepwise structural characterization at three levels	736:787	The CIs of antibodies were fractionated by strong cation exchange chromatography and verified by capillary isoelectric focusing-whole column imaging detection, followed by stepwise structural characterization at three levels.
35573890	3	76	theme	CIs	459:461	arg1	status					449:454	the posttranslational modification status	414:454	the posttranslational modification status of CIs of antibody drugs	414:479	We intended to quantitatively characterize the posttranslational modification status of CIs of antibody drugs and explore the impact of posttranslational modifications on charge heterogeneity.
35573890	12	77	theme	analyses	1686:1693	arg1	importance					1654:1663	the importance	1650:1663	the importance of the comprehensive analyses of antibody CIs	1650:1709	This study demonstrated the importance of the comprehensive analyses of antibody CIs and provides a reference method for the quality control of biopharmaceutical analysis.
35573890	6	78	theme	deamidation	1039:1049	arg1	modifications					1011:1023	common modifications	1004:1023	common modifications of oxidation, deamidation, and glycosylation	1004:1068	Second, at the peptide level, common modifications of oxidation, deamidation, and glycosylation were identified.
35573890	2	79	theme	comprehensive	243:255	arg1	mapping					257:263	The comprehensive mapping	239:263	The comprehensive mapping of antibody modifications and different charge isomers (CIs)	239:324	The comprehensive mapping of antibody modifications and different charge isomers (CIs) is of utmost importance, but is challenging.
35573890	3	80	theme	charge	542:547	arg1	heterogeneity					549:561	charge heterogeneity	542:561	charge heterogeneity	542:561	We intended to quantitatively characterize the posttranslational modification status of CIs of antibody drugs and explore the impact of posttranslational modifications on charge heterogeneity.
35573890	6	81	theme	glycosylation	1056:1068	arg1	modifications					1011:1023	common modifications	1004:1023	common modifications of oxidation, deamidation, and glycosylation	1004:1068	Second, at the peptide level, common modifications of oxidation, deamidation, and glycosylation were identified.
31242281	0	0	theme	oomycete	78:85	arg1	colonization					87:98	oomycete colonization	78:98	oomycete colonization	78:98	Extensin arabinosylation is involved in root response to elicitors and limits oomycete colonization.
31242281	6	1	from	effect	964:969	arg1	colonization					1007:1018	root colonization	1002:1018	root colonization	1002:1018	Roots were also inoculated with a soilborne oomycete, Phytophthora parasitica, to assess the effect of extensin arabinosylation on root colonization.
31242281	4	2	used	used	569:572	arg2	We					566:567	We	566:567	We	566:567	METHODS We used Arabidopsis thaliana mutants impaired in extensin arabinosylation to investigate the role of extensin arabinosylation in root-microbe interactions.
31242281	10	3	theme	pathogenic	1585:1594	arg1	oomycetes					1596:1604	pathogenic oomycetes	1585:1604	pathogenic oomycetes	1585:1604	CONCLUSIONS We provide evidence for a link between extensin arabinosylation and root defence, and propose a model to explain the importance of glycosylation in limiting invasion of root cells by pathogenic oomycetes.
31242281	2	4	theme	correct	361:367	arg1	conformation					380:391	the correct structural conformation	357:391	the correct structural conformation	357:391	The glycan moiety of extensins is believed to confer the correct structural conformation to the glycoprotein, leading to self-assembly within the cell wall that helps limit microbial adherence and invasion.
31242281	8	5	theme	epitope	1177:1183	arg1	expression					1185:1194	altered epitope expression	1169:1194	altered epitope expression in mutant roots	1169:1210	Elicitation also triggers altered epitope expression in mutant roots compared with wild-type and non-elicited roots.
31242281	7	6	dep	elicitation	1130:1140	arg1	response					1118:1125	response	1118:1125	response	1118:1125	KEY RESULTS A differential distribution of extensin epitopes was observed in wild-type plants in response to elicitation.
31242281	9	7	dep	pathogen	1281:1288	arg1	parasitica					1293:1302	the pathogen P. parasitica	1277:1302	the pathogen P. parasitica	1277:1302	Inoculation with the pathogen P. parasitica resulted in enhanced root colonization for two mutants, specifically xeg113 and rra2.
31242281	4	8	theme	thaliana	586:593	arg1	mutants					595:601	Arabidopsis thaliana mutants	574:601	Arabidopsis thaliana mutants impaired in extensin arabinosylation	574:638	METHODS We used Arabidopsis thaliana mutants impaired in extensin arabinosylation to investigate the role of extensin arabinosylation in root-microbe interactions.
31242281	9	9	theme	enhanced	1316:1323	arg1	colonization					1330:1341	enhanced root colonization	1316:1341	enhanced root colonization for two mutants, specifically xeg113 and rra2	1316:1387	Inoculation with the pathogen P. parasitica resulted in enhanced root colonization for two mutants, specifically xeg113 and rra2.
31242281	7	10	theme	KEY	1021:1023	arg1	RESULTS					1025:1031	KEY RESULTS	1021:1031	KEY RESULTS A differential distribution of extensin epitopes was observed in wild-type plants in response to elicitation.	1021:1141	KEY RESULTS A differential distribution of extensin epitopes was observed in wild-type plants in response to elicitation.
31242281	8	11	theme	wild-type	1226:1234	arg1	roots					1253:1257	wild-type and non-elicited roots	1226:1257	wild-type and non-elicited roots	1226:1257	Elicitation also triggers altered epitope expression in mutant roots compared with wild-type and non-elicited roots.
31242281	7	12	located	observed	1086:1093	arg1	plants					1108:1113	wild-type plants	1098:1113	wild-type plants	1098:1113	KEY RESULTS A differential distribution of extensin epitopes was observed in wild-type plants in response to elicitation.
31242281	7	12	located	observed	1086:1093	arg2	distribution					1048:1059	A differential distribution	1033:1059	A differential distribution of extensin epitopes	1033:1080	KEY RESULTS A differential distribution of extensin epitopes was observed in wild-type plants in response to elicitation.
31242281	7	12	located	observed	1086:1093	arg1	elicitation					1130:1140	elicitation	1130:1140	elicitation	1130:1140	KEY RESULTS A differential distribution of extensin epitopes was observed in wild-type plants in response to elicitation.
31242281	5	13	theme	immune	778:783	arg1	response					785:792	an immune response	775:792	an immune response with flagellin 22	775:810	Mutant and wild-type roots were stimulated to elicit an immune response with flagellin 22 and immunolabelled with a set of anti-extensin antibodies.
31242281	2	14	gly	glycoprotein	400:411	arg1	glycoprotein					400:411	the glycoprotein	396:411	the glycoprotein	396:411	The glycan moiety of extensins is believed to confer the correct structural conformation to the glycoprotein, leading to self-assembly within the cell wall that helps limit microbial adherence and invasion.
31242281	10	15	theme	root	1470:1473	arg1	defence					1475:1481	root defence	1470:1481	root defence	1470:1481	CONCLUSIONS We provide evidence for a link between extensin arabinosylation and root defence, and propose a model to explain the importance of glycosylation in limiting invasion of root cells by pathogenic oomycetes.
31242281	8	16	theme	non-elicited	1240:1251	arg1	roots					1253:1257	wild-type and non-elicited roots	1226:1257	wild-type and non-elicited roots	1226:1257	Elicitation also triggers altered epitope expression in mutant roots compared with wild-type and non-elicited roots.
31242281	6	17	theme	Phytophthora	925:936	arg1	parasitica					938:947	Phytophthora parasitica	925:947	Phytophthora parasitica	925:947	Roots were also inoculated with a soilborne oomycete, Phytophthora parasitica, to assess the effect of extensin arabinosylation on root colonization.
31242281	6	17	theme	Phytophthora	925:936	arg1	oomycete					915:922	a soilborne oomycete	903:922	a soilborne oomycete	903:922	Roots were also inoculated with a soilborne oomycete, Phytophthora parasitica, to assess the effect of extensin arabinosylation on root colonization.
31242281	4	18	theme	extensin	615:622	arg1	arabinosylation					624:638	extensin arabinosylation	615:638	extensin arabinosylation	615:638	METHODS We used Arabidopsis thaliana mutants impaired in extensin arabinosylation to investigate the role of extensin arabinosylation in root-microbe interactions.
31242281	1	19	theme	plant	195:199	arg1	one					212:214	one	212:214	one	212:214	BACKGROUND AND AIMS Extensins are hydroxyproline-rich glycoproteins thought to strengthen the plant cell wall, one of the first barriers against pathogens, through intra- and intermolecular cross-links.
31242281	1	19	theme	plant	195:199	arg1	wall					206:209	the plant cell wall	191:209	the plant cell wall	191:209	BACKGROUND AND AIMS Extensins are hydroxyproline-rich glycoproteins thought to strengthen the plant cell wall, one of the first barriers against pathogens, through intra- and intermolecular cross-links.
31242281	1	19	theme	plant	195:199	arg1	barriers					229:236	the first barriers	219:236	the first barriers against pathogens	219:254	BACKGROUND AND AIMS Extensins are hydroxyproline-rich glycoproteins thought to strengthen the plant cell wall, one of the first barriers against pathogens, through intra- and intermolecular cross-links.
31242281	4	20	dep	METHODS	558:564	arg1	used					569:572	used	569:572	used Arabidopsis thaliana mutants impaired in extensin arabinosylation to investigate the role of extensin arabinosylation in root-microbe interactions	569:719	METHODS We used Arabidopsis thaliana mutants impaired in extensin arabinosylation to investigate the role of extensin arabinosylation in root-microbe interactions.
31242281	9	21	theme	P.	1290:1291	arg1	pathogen					1281:1288	the pathogen P. parasitica	1277:1302	the pathogen P. parasitica	1277:1302	Inoculation with the pathogen P. parasitica resulted in enhanced root colonization for two mutants, specifically xeg113 and rra2.
31242281	2	22	theme	extensins	325:333	arg1	moiety					315:320	The glycan moiety	304:320	The glycan moiety of extensins	304:333	The glycan moiety of extensins is believed to confer the correct structural conformation to the glycoprotein, leading to self-assembly within the cell wall that helps limit microbial adherence and invasion.
31242281	5	23	theme	Mutant	722:727	arg1	roots					743:747	Mutant and wild-type roots	722:747	Mutant and wild-type roots	722:747	Mutant and wild-type roots were stimulated to elicit an immune response with flagellin 22 and immunolabelled with a set of anti-extensin antibodies.
31242281	1	24	theme	cell	201:204	arg1	one					212:214	one	212:214	one	212:214	BACKGROUND AND AIMS Extensins are hydroxyproline-rich glycoproteins thought to strengthen the plant cell wall, one of the first barriers against pathogens, through intra- and intermolecular cross-links.
31242281	1	24	theme	cell	201:204	arg1	wall					206:209	the plant cell wall	191:209	the plant cell wall	191:209	BACKGROUND AND AIMS Extensins are hydroxyproline-rich glycoproteins thought to strengthen the plant cell wall, one of the first barriers against pathogens, through intra- and intermolecular cross-links.
31242281	1	24	theme	cell	201:204	arg1	barriers					229:236	the first barriers	219:236	the first barriers against pathogens	219:254	BACKGROUND AND AIMS Extensins are hydroxyproline-rich glycoproteins thought to strengthen the plant cell wall, one of the first barriers against pathogens, through intra- and intermolecular cross-links.
31242281	0	25	theme	Extensin	0:7	arg1	arabinosylation					9:23	Extensin arabinosylation	0:23	Extensin arabinosylation	0:23	Extensin arabinosylation is involved in root response to elicitors and limits oomycete colonization.
31242281	8	26	theme	altered	1169:1175	arg1	expression					1185:1194	altered epitope expression	1169:1194	altered epitope expression in mutant roots	1169:1210	Elicitation also triggers altered epitope expression in mutant roots compared with wild-type and non-elicited roots.
31242281	10	27	theme	glycosylation	1533:1545	arg1	importance					1519:1528	the importance	1515:1528	the importance of glycosylation in limiting invasion of root cells by pathogenic oomycetes	1515:1604	CONCLUSIONS We provide evidence for a link between extensin arabinosylation and root defence, and propose a model to explain the importance of glycosylation in limiting invasion of root cells by pathogenic oomycetes.
31242281	7	28	dep	RESULTS	1025:1031	arg1	observed					1086:1093	observed	1086:1093	was observed in wild-type plants in response to elicitation	1082:1140	KEY RESULTS A differential distribution of extensin epitopes was observed in wild-type plants in response to elicitation.
31242281	4	29	theme	root-microbe	695:706	arg1	interactions					708:719	root-microbe interactions	695:719	root-microbe interactions	695:719	METHODS We used Arabidopsis thaliana mutants impaired in extensin arabinosylation to investigate the role of extensin arabinosylation in root-microbe interactions.
31242281	2	30	theme	cell	450:453	arg1	wall					455:458	the cell wall	446:458	the cell wall that helps limit microbial adherence and invasion	446:508	The glycan moiety of extensins is believed to confer the correct structural conformation to the glycoprotein, leading to self-assembly within the cell wall that helps limit microbial adherence and invasion.
31242281	5	31	theme	anti-extensin	845:857	arg1	antibodies					859:868	anti-extensin antibodies	845:868	anti-extensin antibodies	845:868	Mutant and wild-type roots were stimulated to elicit an immune response with flagellin 22 and immunolabelled with a set of anti-extensin antibodies.
31242281	4	32	theme	Arabidopsis	574:584	arg1	mutants					595:601	Arabidopsis thaliana mutants	574:601	Arabidopsis thaliana mutants impaired in extensin arabinosylation	574:638	METHODS We used Arabidopsis thaliana mutants impaired in extensin arabinosylation to investigate the role of extensin arabinosylation in root-microbe interactions.
31242281	10	33	theme	cells	1576:1580	arg1	invasion					1559:1566	invasion	1559:1566	invasion of root cells by pathogenic oomycetes	1559:1604	CONCLUSIONS We provide evidence for a link between extensin arabinosylation and root defence, and propose a model to explain the importance of glycosylation in limiting invasion of root cells by pathogenic oomycetes.
31242281	2	34	theme	glycan	308:313	arg1	moiety					315:320	The glycan moiety	304:320	The glycan moiety of extensins	304:333	The glycan moiety of extensins is believed to confer the correct structural conformation to the glycoprotein, leading to self-assembly within the cell wall that helps limit microbial adherence and invasion.
31242281	4	35	from	role	659:662	arg1	interactions					708:719	root-microbe interactions	695:719	root-microbe interactions	695:719	METHODS We used Arabidopsis thaliana mutants impaired in extensin arabinosylation to investigate the role of extensin arabinosylation in root-microbe interactions.
31242281	0	36	theme	root	40:43	arg1	response					45:52	root response	40:52	root response to elicitors	40:65	Extensin arabinosylation is involved in root response to elicitors and limits oomycete colonization.
31242281	6	37	theme	root	1002:1005	arg1	colonization					1007:1018	root colonization	1002:1018	root colonization	1002:1018	Roots were also inoculated with a soilborne oomycete, Phytophthora parasitica, to assess the effect of extensin arabinosylation on root colonization.
31242281	10	38	theme	root	1571:1574	arg1	cells					1576:1580	root cells	1571:1580	root cells	1571:1580	CONCLUSIONS We provide evidence for a link between extensin arabinosylation and root defence, and propose a model to explain the importance of glycosylation in limiting invasion of root cells by pathogenic oomycetes.
31242281	6	39	theme	soilborne	905:913	arg1	parasitica					938:947	Phytophthora parasitica	925:947	Phytophthora parasitica	925:947	Roots were also inoculated with a soilborne oomycete, Phytophthora parasitica, to assess the effect of extensin arabinosylation on root colonization.
31242281	6	39	theme	soilborne	905:913	arg1	oomycete					915:922	a soilborne oomycete	903:922	a soilborne oomycete	903:922	Roots were also inoculated with a soilborne oomycete, Phytophthora parasitica, to assess the effect of extensin arabinosylation on root colonization.
31242281	4	40	theme	extensin	667:674	arg1	arabinosylation					676:690	extensin arabinosylation	667:690	extensin arabinosylation	667:690	METHODS We used Arabidopsis thaliana mutants impaired in extensin arabinosylation to investigate the role of extensin arabinosylation in root-microbe interactions.
31242281	2	41	theme	microbial	477:485	arg1	adherence					487:495	microbial adherence	477:495	microbial adherence	477:495	The glycan moiety of extensins is believed to confer the correct structural conformation to the glycoprotein, leading to self-assembly within the cell wall that helps limit microbial adherence and invasion.
31242281	8	42	from	expression	1185:1194	arg1	roots					1206:1210	mutant roots	1199:1210	mutant roots	1199:1210	Elicitation also triggers altered epitope expression in mutant roots compared with wild-type and non-elicited roots.
31242281	9	43	theme	root	1325:1328	arg1	colonization					1330:1341	enhanced root colonization	1316:1341	enhanced root colonization for two mutants, specifically xeg113 and rra2	1316:1387	Inoculation with the pathogen P. parasitica resulted in enhanced root colonization for two mutants, specifically xeg113 and rra2.
31242281	5	44	theme	antibodies	859:868	arg1	set					838:840	a set	836:840	a set of anti-extensin antibodies	836:868	Mutant and wild-type roots were stimulated to elicit an immune response with flagellin 22 and immunolabelled with a set of anti-extensin antibodies.
31242281	5	44	theme	antibodies	859:868	arg1	antibodies					859:868	anti-extensin antibodies	845:868	anti-extensin antibodies	845:868	Mutant and wild-type roots were stimulated to elicit an immune response with flagellin 22 and immunolabelled with a set of anti-extensin antibodies.
31242281	7	45	theme	epitopes	1073:1080	arg1	distribution					1048:1059	A differential distribution	1033:1059	A differential distribution of extensin epitopes	1033:1080	KEY RESULTS A differential distribution of extensin epitopes was observed in wild-type plants in response to elicitation.
31242281	6	46	theme	arabinosylation	983:997	arg1	effect					964:969	the effect	960:969	the effect of extensin arabinosylation on root colonization	960:1018	Roots were also inoculated with a soilborne oomycete, Phytophthora parasitica, to assess the effect of extensin arabinosylation on root colonization.
31242281	7	47	theme	wild-type	1098:1106	arg1	plants					1108:1113	wild-type plants	1098:1113	wild-type plants	1098:1113	KEY RESULTS A differential distribution of extensin epitopes was observed in wild-type plants in response to elicitation.
31242281	6	48	theme	extensin	974:981	arg1	arabinosylation					983:997	extensin arabinosylation	974:997	extensin arabinosylation	974:997	Roots were also inoculated with a soilborne oomycete, Phytophthora parasitica, to assess the effect of extensin arabinosylation on root colonization.
31242281	1	49	gly	glycoproteins	155:167	arg1	AIMS					116:119	AIMS	116:119	AIMS	116:119	BACKGROUND AND AIMS Extensins are hydroxyproline-rich glycoproteins thought to strengthen the plant cell wall, one of the first barriers against pathogens, through intra- and intermolecular cross-links.
31242281	1	49	gly	glycoproteins	155:167	arg1	BACKGROUND					101:110	BACKGROUND	101:110	BACKGROUND	101:110	BACKGROUND AND AIMS Extensins are hydroxyproline-rich glycoproteins thought to strengthen the plant cell wall, one of the first barriers against pathogens, through intra- and intermolecular cross-links.
31242281	1	49	gly	glycoproteins	155:167	arg1	glycoproteins					155:167	hydroxyproline-rich glycoproteins	135:167	hydroxyproline-rich glycoproteins thought to strengthen the plant cell wall, one of the first barriers against pathogens, through intra- and intermolecular cross-links	135:301	BACKGROUND AND AIMS Extensins are hydroxyproline-rich glycoproteins thought to strengthen the plant cell wall, one of the first barriers against pathogens, through intra- and intermolecular cross-links.
31242281	1	50	dep	intra-	265:270	arg1	cross-links					291:301	cross-links	291:301	cross-links	291:301	BACKGROUND AND AIMS Extensins are hydroxyproline-rich glycoproteins thought to strengthen the plant cell wall, one of the first barriers against pathogens, through intra- and intermolecular cross-links.
31242281	9	51	with	Inoculation	1260:1270	arg1	pathogen					1281:1288	the pathogen P. parasitica	1277:1302	the pathogen P. parasitica	1277:1302	Inoculation with the pathogen P. parasitica resulted in enhanced root colonization for two mutants, specifically xeg113 and rra2.
31242281	5	52	with	response	785:792	arg1	flagellin					799:807	flagellin 22	799:810	flagellin 22	799:810	Mutant and wild-type roots were stimulated to elicit an immune response with flagellin 22 and immunolabelled with a set of anti-extensin antibodies.
31242281	1	53	theme	first	223:227	arg1	barriers					229:236	the first barriers	219:236	the first barriers against pathogens	219:254	BACKGROUND AND AIMS Extensins are hydroxyproline-rich glycoproteins thought to strengthen the plant cell wall, one of the first barriers against pathogens, through intra- and intermolecular cross-links.
31242281	5	54	theme	wild-type	733:741	arg1	roots					743:747	Mutant and wild-type roots	722:747	Mutant and wild-type roots	722:747	Mutant and wild-type roots were stimulated to elicit an immune response with flagellin 22 and immunolabelled with a set of anti-extensin antibodies.
31242281	10	55	dep	CONCLUSIONS	1390:1400	arg1	propose					1488:1494	propose	1488:1494	propose a model to explain the importance of glycosylation in limiting invasion of root cells by pathogenic oomycetes	1488:1604	CONCLUSIONS We provide evidence for a link between extensin arabinosylation and root defence, and propose a model to explain the importance of glycosylation in limiting invasion of root cells by pathogenic oomycetes.
31242281	10	55	dep	CONCLUSIONS	1390:1400	arg1	provide					1405:1411	provide	1405:1411	provide evidence for a link between extensin arabinosylation and root defence	1405:1481	CONCLUSIONS We provide evidence for a link between extensin arabinosylation and root defence, and propose a model to explain the importance of glycosylation in limiting invasion of root cells by pathogenic oomycetes.
31242281	7	56	theme	differential	1035:1046	arg1	distribution					1048:1059	A differential distribution	1033:1059	A differential distribution of extensin epitopes	1033:1080	KEY RESULTS A differential distribution of extensin epitopes was observed in wild-type plants in response to elicitation.
31242281	1	57	theme	hydroxyproline-rich	135:153	arg1	AIMS					116:119	AIMS	116:119	AIMS	116:119	BACKGROUND AND AIMS Extensins are hydroxyproline-rich glycoproteins thought to strengthen the plant cell wall, one of the first barriers against pathogens, through intra- and intermolecular cross-links.
31242281	1	57	theme	hydroxyproline-rich	135:153	arg1	BACKGROUND					101:110	BACKGROUND	101:110	BACKGROUND	101:110	BACKGROUND AND AIMS Extensins are hydroxyproline-rich glycoproteins thought to strengthen the plant cell wall, one of the first barriers against pathogens, through intra- and intermolecular cross-links.
31242281	1	57	theme	hydroxyproline-rich	135:153	arg1	glycoproteins					155:167	hydroxyproline-rich glycoproteins	135:167	hydroxyproline-rich glycoproteins thought to strengthen the plant cell wall, one of the first barriers against pathogens, through intra- and intermolecular cross-links	135:301	BACKGROUND AND AIMS Extensins are hydroxyproline-rich glycoproteins thought to strengthen the plant cell wall, one of the first barriers against pathogens, through intra- and intermolecular cross-links.
31242281	1	58	theme	barriers	229:236	arg1	one					212:214	one	212:214	one	212:214	BACKGROUND AND AIMS Extensins are hydroxyproline-rich glycoproteins thought to strengthen the plant cell wall, one of the first barriers against pathogens, through intra- and intermolecular cross-links.
31242281	1	58	theme	barriers	229:236	arg1	wall					206:209	the plant cell wall	191:209	the plant cell wall	191:209	BACKGROUND AND AIMS Extensins are hydroxyproline-rich glycoproteins thought to strengthen the plant cell wall, one of the first barriers against pathogens, through intra- and intermolecular cross-links.
31242281	1	58	theme	barriers	229:236	arg1	barriers					229:236	the first barriers	219:236	the first barriers against pathogens	219:254	BACKGROUND AND AIMS Extensins are hydroxyproline-rich glycoproteins thought to strengthen the plant cell wall, one of the first barriers against pathogens, through intra- and intermolecular cross-links.
31242281	7	59	theme	extensin	1064:1071	arg1	epitopes					1073:1080	extensin epitopes	1064:1080	extensin epitopes	1064:1080	KEY RESULTS A differential distribution of extensin epitopes was observed in wild-type plants in response to elicitation.
31242281	1	60	dep	BACKGROUND	101:110	arg1	Extensins					121:129	Extensins	121:129	Extensins	121:129	BACKGROUND AND AIMS Extensins are hydroxyproline-rich glycoproteins thought to strengthen the plant cell wall, one of the first barriers against pathogens, through intra- and intermolecular cross-links.
31242281	4	61	theme	arabinosylation	676:690	arg1	role					659:662	the role	655:662	the role of extensin arabinosylation in root-microbe interactions	655:719	METHODS We used Arabidopsis thaliana mutants impaired in extensin arabinosylation to investigate the role of extensin arabinosylation in root-microbe interactions.
31242281	8	62	theme	mutant	1199:1204	arg1	roots					1206:1210	mutant roots	1199:1210	mutant roots	1199:1210	Elicitation also triggers altered epitope expression in mutant roots compared with wild-type and non-elicited roots.
31242281	10	63	theme	extensin	1441:1448	arg1	arabinosylation					1450:1464	extensin arabinosylation	1441:1464	extensin arabinosylation	1441:1464	CONCLUSIONS We provide evidence for a link between extensin arabinosylation and root defence, and propose a model to explain the importance of glycosylation in limiting invasion of root cells by pathogenic oomycetes.
31242281	2	64	theme	structural	369:378	arg1	conformation					380:391	the correct structural conformation	357:391	the correct structural conformation	357:391	The glycan moiety of extensins is believed to confer the correct structural conformation to the glycoprotein, leading to self-assembly within the cell wall that helps limit microbial adherence and invasion.
33708211	5	0	theme	in	807:808	arg1	recruitment					748:758	neutrophil recruitment	737:758	neutrophil recruitment under basal conditions and during inflammation in vivo	737:813	Moreover, the impact of fiber deficiency on neutrophil recruitment under basal conditions and during inflammation in vivo is unknown.
33708211	8	1	theme	no-fiber	1193:1200	arg1	diet					1202:1205	a no-fiber diet	1191:1205	a no-fiber diet	1191:1205	Although mice fed a no-fiber diet alone did not have observable colitis-associated symptoms, these animals were highly susceptible to low dose (0.5%) dextran sodium sulphate (DSS)-induced model of colitis.
33708211	7	2	theme	no-fiber	1005:1012	arg1	diet					1014:1017	a no-fiber diet	1003:1017	a no-fiber diet for 14 days	1003:1029	Mice fed a no-fiber diet for 14 days showed significant changes in the gut microbiota and exhibited increased neutrophil-endothelial interactions in the colonic microvasculature.
33708211	8	3	theme	-induced	1352:1359	arg1	model					1361:1365	dextran sodium sulphate (DSS)-induced model	1323:1365	low dose (0.5%) dextran sodium sulphate (DSS)-induced model of colitis	1307:1376	Although mice fed a no-fiber diet alone did not have observable colitis-associated symptoms, these animals were highly susceptible to low dose (0.5%) dextran sodium sulphate (DSS)-induced model of colitis.
33708211	9	4	theme	no-fiber	1441:1448	arg1	enrichment					1464:1473	no-fiber diet-mediated enrichment	1441:1473	no-fiber diet-mediated enrichment of colonic neutrophils and colitis pathology	1441:1518	Supplementation of the most abundant SCFA, acetate, prevented no-fiber diet-mediated enrichment of colonic neutrophils and colitis pathology.
33708211	10	5	theme	dietary	1532:1538	arg1	fiber					1540:1544	dietary fiber	1532:1544	dietary fiber	1532:1544	Therefore, dietary fiber, possibly through the actions of acetate, plays an important role in regulating neutrophil recruitment and host protection against inflammatory colonic damage in an experimental model of colitis.
33708211	4	6	theme	initial	601:607	arg1	steps					609:613	the key initial steps	593:613	the key initial steps	593:613	However, the effect of fiber deficiency on the key initial steps of inflammation, such as leukocyte-endothelial cell interactions, is unknown.
33708211	4	6	theme	initial	601:607	arg1	interactions					667:678	leukocyte-endothelial cell interactions	640:678	leukocyte-endothelial cell interactions	640:678	However, the effect of fiber deficiency on the key initial steps of inflammation, such as leukocyte-endothelial cell interactions, is unknown.
33708211	0	7	theme	Experimental	101:112	arg1	Colitis					114:120	Experimental Colitis	101:120	Experimental Colitis	101:120	Deficiency of Dietary Fiber Modulates Gut Microbiota Composition, Neutrophil Recruitment and Worsens Experimental Colitis.
33708211	7	8	theme	colonic	1147:1153	arg1	microvasculature					1155:1170	the colonic microvasculature	1143:1170	the colonic microvasculature	1143:1170	Mice fed a no-fiber diet for 14 days showed significant changes in the gut microbiota and exhibited increased neutrophil-endothelial interactions in the colonic microvasculature.
33708211	2	9	theme	gut	305:307	arg1	microbiota					309:318	the gut microbiota	301:318	the gut microbiota	301:318	Recent evidence indicates that diet alters the composition of the gut microbiota and influences host-pathogen interactions.
33708211	4	10	theme	inflammation	618:629	arg1	steps					609:613	the key initial steps	593:613	the key initial steps	593:613	However, the effect of fiber deficiency on the key initial steps of inflammation, such as leukocyte-endothelial cell interactions, is unknown.
33708211	4	10	theme	inflammation	618:629	arg1	interactions					667:678	leukocyte-endothelial cell interactions	640:678	leukocyte-endothelial cell interactions	640:678	However, the effect of fiber deficiency on the key initial steps of inflammation, such as leukocyte-endothelial cell interactions, is unknown.
33708211	3	11	theme	dietary	403:409	arg1	fiber					411:415	dietary fiber	403:415	dietary fiber	403:415	Specifically, bacterial fermentation of dietary fiber produces metabolites called short-chain fatty acids (SCFAs), which have been shown to protect against various inflammatory diseases.
33708211	10	12	theme	host	1653:1656	arg1	protection					1658:1667	host protection	1653:1667	host protection	1653:1667	Therefore, dietary fiber, possibly through the actions of acetate, plays an important role in regulating neutrophil recruitment and host protection against inflammatory colonic damage in an experimental model of colitis.
33708211	9	13	theme	diet-mediated	1450:1462	arg1	enrichment					1464:1473	no-fiber diet-mediated enrichment	1441:1473	no-fiber diet-mediated enrichment of colonic neutrophils and colitis pathology	1441:1518	Supplementation of the most abundant SCFA, acetate, prevented no-fiber diet-mediated enrichment of colonic neutrophils and colitis pathology.
33708211	3	14	theme	bacterial	377:385	arg1	fermentation					387:398	bacterial fermentation	377:398	bacterial fermentation of dietary fiber	377:415	Specifically, bacterial fermentation of dietary fiber produces metabolites called short-chain fatty acids (SCFAs), which have been shown to protect against various inflammatory diseases.
33708211	3	15	theme	various	519:525	arg1	diseases					540:547	various inflammatory diseases	519:547	various inflammatory diseases	519:547	Specifically, bacterial fermentation of dietary fiber produces metabolites called short-chain fatty acids (SCFAs), which have been shown to protect against various inflammatory diseases.
33708211	4	16	theme	cell	662:665	arg1	interactions					667:678	leukocyte-endothelial cell interactions	640:678	leukocyte-endothelial cell interactions	640:678	However, the effect of fiber deficiency on the key initial steps of inflammation, such as leukocyte-endothelial cell interactions, is unknown.
33708211	10	17	theme	acetate	1579:1585	arg1	actions					1568:1574	the actions	1564:1574	the actions of acetate	1564:1585	Therefore, dietary fiber, possibly through the actions of acetate, plays an important role in regulating neutrophil recruitment and host protection against inflammatory colonic damage in an experimental model of colitis.
33708211	8	18	theme	colitis-associated	1237:1254	arg1	symptoms					1256:1263	observable colitis-associated symptoms	1226:1263	observable colitis-associated symptoms	1226:1263	Although mice fed a no-fiber diet alone did not have observable colitis-associated symptoms, these animals were highly susceptible to low dose (0.5%) dextran sodium sulphate (DSS)-induced model of colitis.
33708211	1	19	theme	colon	176:180	arg1	disease					161:167	an inflammatory disease	145:167	an inflammatory disease of the colon that is associated with colonic neutrophil accumulation	145:236	Ulcerative colitis is an inflammatory disease of the colon that is associated with colonic neutrophil accumulation.
33708211	1	19	theme	colon	176:180	arg1	colitis					134:140	Ulcerative colitis	123:140	Ulcerative colitis	123:140	Ulcerative colitis is an inflammatory disease of the colon that is associated with colonic neutrophil accumulation.
33708211	0	20	theme	Microbiota	42:51	arg1	Recruitment					77:87	Neutrophil Recruitment	66:87	Neutrophil Recruitment	66:87	Deficiency of Dietary Fiber Modulates Gut Microbiota Composition, Neutrophil Recruitment and Worsens Experimental Colitis.
33708211	0	20	theme	Microbiota	42:51	arg1	Composition					53:63	Gut Microbiota Composition	38:63	Gut Microbiota Composition	38:63	Deficiency of Dietary Fiber Modulates Gut Microbiota Composition, Neutrophil Recruitment and Worsens Experimental Colitis.
33708211	10	21	theme	experimental	1711:1722	arg1	model					1724:1728	an experimental model	1708:1728	an experimental model of colitis	1708:1739	Therefore, dietary fiber, possibly through the actions of acetate, plays an important role in regulating neutrophil recruitment and host protection against inflammatory colonic damage in an experimental model of colitis.
33708211	8	22	theme	colitis	1370:1376	arg1	model					1361:1365	dextran sodium sulphate (DSS)-induced model	1323:1365	low dose (0.5%) dextran sodium sulphate (DSS)-induced model of colitis	1307:1376	Although mice fed a no-fiber diet alone did not have observable colitis-associated symptoms, these animals were highly susceptible to low dose (0.5%) dextran sodium sulphate (DSS)-induced model of colitis.
33708211	8	23	theme	observable	1226:1235	arg1	symptoms					1256:1263	observable colitis-associated symptoms	1226:1263	observable colitis-associated symptoms	1226:1263	Although mice fed a no-fiber diet alone did not have observable colitis-associated symptoms, these animals were highly susceptible to low dose (0.5%) dextran sodium sulphate (DSS)-induced model of colitis.
33708211	7	24	theme	neutrophil-endothelial	1104:1125	arg1	interactions					1127:1138	increased neutrophil-endothelial interactions	1094:1138	increased neutrophil-endothelial interactions in the colonic microvasculature	1094:1170	Mice fed a no-fiber diet for 14 days showed significant changes in the gut microbiota and exhibited increased neutrophil-endothelial interactions in the colonic microvasculature.
33708211	2	25	theme	host-pathogen	335:347	arg1	interactions					349:360	host-pathogen interactions	335:360	host-pathogen interactions	335:360	Recent evidence indicates that diet alters the composition of the gut microbiota and influences host-pathogen interactions.
33708211	7	26	theme	gut	1065:1067	arg1	microbiota					1069:1078	the gut microbiota	1061:1078	the gut microbiota	1061:1078	Mice fed a no-fiber diet for 14 days showed significant changes in the gut microbiota and exhibited increased neutrophil-endothelial interactions in the colonic microvasculature.
33708211	8	27	theme	low	1307:1309	arg1	dose					1311:1314	low dose	1307:1314	low dose (0.5%) dextran sodium sulphate (DSS)-induced model of colitis	1307:1376	Although mice fed a no-fiber diet alone did not have observable colitis-associated symptoms, these animals were highly susceptible to low dose (0.5%) dextran sodium sulphate (DSS)-induced model of colitis.
33708211	8	27	theme	low	1307:1309	arg1	%					1320:1320	0.5%	1317:1320	0.5%	1317:1320	Although mice fed a no-fiber diet alone did not have observable colitis-associated symptoms, these animals were highly susceptible to low dose (0.5%) dextran sodium sulphate (DSS)-induced model of colitis.
33708211	10	28	theme	neutrophil	1626:1635	arg1	recruitment					1637:1647	neutrophil recruitment	1626:1647	neutrophil recruitment	1626:1647	Therefore, dietary fiber, possibly through the actions of acetate, plays an important role in regulating neutrophil recruitment and host protection against inflammatory colonic damage in an experimental model of colitis.
33708211	10	29	theme	colitis	1733:1739	arg1	model					1724:1728	an experimental model	1708:1728	an experimental model of colitis	1708:1739	Therefore, dietary fiber, possibly through the actions of acetate, plays an important role in regulating neutrophil recruitment and host protection against inflammatory colonic damage in an experimental model of colitis.
33708211	9	30	theme	neutrophils	1486:1496	arg1	enrichment					1464:1473	no-fiber diet-mediated enrichment	1441:1473	no-fiber diet-mediated enrichment of colonic neutrophils and colitis pathology	1441:1518	Supplementation of the most abundant SCFA, acetate, prevented no-fiber diet-mediated enrichment of colonic neutrophils and colitis pathology.
33708211	3	31	theme	fiber	411:415	arg1	fermentation					387:398	bacterial fermentation	377:398	bacterial fermentation of dietary fiber	377:415	Specifically, bacterial fermentation of dietary fiber produces metabolites called short-chain fatty acids (SCFAs), which have been shown to protect against various inflammatory diseases.
33708211	4	32	theme	deficiency	579:588	arg1	effect					563:568	the effect	559:568	the effect of fiber deficiency on the key initial steps of inflammation, such as leukocyte-endothelial cell interactions,	559:679	However, the effect of fiber deficiency on the key initial steps of inflammation, such as leukocyte-endothelial cell interactions, is unknown.
33708211	4	32	theme	deficiency	579:588	arg1	unknown					684:690	unknown	684:690	unknown	684:690	However, the effect of fiber deficiency on the key initial steps of inflammation, such as leukocyte-endothelial cell interactions, is unknown.
33708211	5	33	theme	deficiency	723:732	arg1	impact					707:712	the impact	703:712	the impact of fiber deficiency on neutrophil recruitment under basal conditions and during inflammation in vivo	703:813	Moreover, the impact of fiber deficiency on neutrophil recruitment under basal conditions and during inflammation in vivo is unknown.
33708211	5	33	theme	deficiency	723:732	arg1	unknown					818:824	unknown	818:824	unknown	818:824	Moreover, the impact of fiber deficiency on neutrophil recruitment under basal conditions and during inflammation in vivo is unknown.
33708211	10	34	theme	colonic	1690:1696	arg1	damage					1698:1703	inflammatory colonic damage	1677:1703	inflammatory colonic damage in an experimental model of colitis	1677:1739	Therefore, dietary fiber, possibly through the actions of acetate, plays an important role in regulating neutrophil recruitment and host protection against inflammatory colonic damage in an experimental model of colitis.
33708211	8	35	dep	dose	1311:1314	arg1	model					1361:1365	dextran sodium sulphate (DSS)-induced model	1323:1365	low dose (0.5%) dextran sodium sulphate (DSS)-induced model of colitis	1307:1376	Although mice fed a no-fiber diet alone did not have observable colitis-associated symptoms, these animals were highly susceptible to low dose (0.5%) dextran sodium sulphate (DSS)-induced model of colitis.
33708211	9	36	theme	colitis	1502:1508	arg1	pathology					1510:1518	colitis pathology	1502:1518	colitis pathology	1502:1518	Supplementation of the most abundant SCFA, acetate, prevented no-fiber diet-mediated enrichment of colonic neutrophils and colitis pathology.
33708211	0	37	theme	Dietary	14:20	arg1	Fiber					22:26	Dietary Fiber	14:26	Dietary Fiber	14:26	Deficiency of Dietary Fiber Modulates Gut Microbiota Composition, Neutrophil Recruitment and Worsens Experimental Colitis.
33708211	4	38	theme	key	597:599	arg1	steps					609:613	the key initial steps	593:613	the key initial steps	593:613	However, the effect of fiber deficiency on the key initial steps of inflammation, such as leukocyte-endothelial cell interactions, is unknown.
33708211	4	38	theme	key	597:599	arg1	interactions					667:678	leukocyte-endothelial cell interactions	640:678	leukocyte-endothelial cell interactions	640:678	However, the effect of fiber deficiency on the key initial steps of inflammation, such as leukocyte-endothelial cell interactions, is unknown.
33708211	7	39	theme	increased	1094:1102	arg1	interactions					1127:1138	increased neutrophil-endothelial interactions	1094:1138	increased neutrophil-endothelial interactions in the colonic microvasculature	1094:1170	Mice fed a no-fiber diet for 14 days showed significant changes in the gut microbiota and exhibited increased neutrophil-endothelial interactions in the colonic microvasculature.
33708211	2	40	theme	microbiota	309:318	arg1	composition					286:296	the composition	282:296	the composition of the gut microbiota	282:318	Recent evidence indicates that diet alters the composition of the gut microbiota and influences host-pathogen interactions.
33708211	1	41	theme	colonic	206:212	arg1	accumulation					225:236	colonic neutrophil accumulation	206:236	colonic neutrophil accumulation	206:236	Ulcerative colitis is an inflammatory disease of the colon that is associated with colonic neutrophil accumulation.
33708211	0	42	theme	Gut	38:40	arg1	Recruitment					77:87	Neutrophil Recruitment	66:87	Neutrophil Recruitment	66:87	Deficiency of Dietary Fiber Modulates Gut Microbiota Composition, Neutrophil Recruitment and Worsens Experimental Colitis.
33708211	0	42	theme	Gut	38:40	arg1	Composition					53:63	Gut Microbiota Composition	38:63	Gut Microbiota Composition	38:63	Deficiency of Dietary Fiber Modulates Gut Microbiota Composition, Neutrophil Recruitment and Worsens Experimental Colitis.
33708211	5	43	theme	neutrophil	737:746	arg1	recruitment					748:758	neutrophil recruitment	737:758	neutrophil recruitment under basal conditions and during inflammation in vivo	737:813	Moreover, the impact of fiber deficiency on neutrophil recruitment under basal conditions and during inflammation in vivo is unknown.
33708211	9	44	theme	pathology	1510:1518	arg1	enrichment					1464:1473	no-fiber diet-mediated enrichment	1441:1473	no-fiber diet-mediated enrichment of colonic neutrophils and colitis pathology	1441:1518	Supplementation of the most abundant SCFA, acetate, prevented no-fiber diet-mediated enrichment of colonic neutrophils and colitis pathology.
33708211	10	45	theme	inflammatory	1677:1688	arg1	damage					1698:1703	inflammatory colonic damage	1677:1703	inflammatory colonic damage in an experimental model of colitis	1677:1739	Therefore, dietary fiber, possibly through the actions of acetate, plays an important role in regulating neutrophil recruitment and host protection against inflammatory colonic damage in an experimental model of colitis.
33708211	1	46	theme	neutrophil	214:223	arg1	accumulation					225:236	colonic neutrophil accumulation	206:236	colonic neutrophil accumulation	206:236	Ulcerative colitis is an inflammatory disease of the colon that is associated with colonic neutrophil accumulation.
33708211	6	47	theme	colitis	975:981	arg1	pathology					983:991	more severe colitis pathology	963:991	more severe colitis pathology	963:991	Herein, we hypothesized that a fiber-deficient diet promotes an inflammatory state in the colon at baseline and predisposes the host to more severe colitis pathology.
33708211	6	48	theme	inflammatory	891:902	arg1	state					904:908	an inflammatory state	888:908	an inflammatory state in the colon	888:921	Herein, we hypothesized that a fiber-deficient diet promotes an inflammatory state in the colon at baseline and predisposes the host to more severe colitis pathology.
33708211	0	49	theme	Fiber	22:26	arg1	Deficiency					0:9	Deficiency	0:9	Deficiency of Dietary Fiber	0:26	Deficiency of Dietary Fiber Modulates Gut Microbiota Composition, Neutrophil Recruitment and Worsens Experimental Colitis.
33708211	3	50	theme	short-chain	445:455	arg1	SCFAs					470:474	SCFAs	470:474	SCFAs	470:474	Specifically, bacterial fermentation of dietary fiber produces metabolites called short-chain fatty acids (SCFAs), which have been shown to protect against various inflammatory diseases.
33708211	3	50	theme	short-chain	445:455	arg1	acids					463:467	short-chain fatty acids	445:467	short-chain fatty acids (SCFAs)	445:475	Specifically, bacterial fermentation of dietary fiber produces metabolites called short-chain fatty acids (SCFAs), which have been shown to protect against various inflammatory diseases.
33708211	6	51	theme	severe	968:973	arg1	pathology					983:991	more severe colitis pathology	963:991	more severe colitis pathology	963:991	Herein, we hypothesized that a fiber-deficient diet promotes an inflammatory state in the colon at baseline and predisposes the host to more severe colitis pathology.
33708211	7	52	from	interactions	1127:1138	arg1	microvasculature					1155:1170	the colonic microvasculature	1143:1170	the colonic microvasculature	1143:1170	Mice fed a no-fiber diet for 14 days showed significant changes in the gut microbiota and exhibited increased neutrophil-endothelial interactions in the colonic microvasculature.
33708211	2	53	theme	Recent	239:244	arg1	evidence					246:253	Recent evidence	239:253	Recent evidence	239:253	Recent evidence indicates that diet alters the composition of the gut microbiota and influences host-pathogen interactions.
33708211	7	54	theme	significant	1038:1048	arg1	changes					1050:1056	significant changes	1038:1056	significant changes in the gut microbiota	1038:1078	Mice fed a no-fiber diet for 14 days showed significant changes in the gut microbiota and exhibited increased neutrophil-endothelial interactions in the colonic microvasculature.
33708211	1	55	theme	Ulcerative	123:132	arg1	disease					161:167	an inflammatory disease	145:167	an inflammatory disease of the colon that is associated with colonic neutrophil accumulation	145:236	Ulcerative colitis is an inflammatory disease of the colon that is associated with colonic neutrophil accumulation.
33708211	1	55	theme	Ulcerative	123:132	arg1	colitis					134:140	Ulcerative colitis	123:140	Ulcerative colitis	123:140	Ulcerative colitis is an inflammatory disease of the colon that is associated with colonic neutrophil accumulation.
33708211	3	56	theme	fatty	457:461	arg1	SCFAs					470:474	SCFAs	470:474	SCFAs	470:474	Specifically, bacterial fermentation of dietary fiber produces metabolites called short-chain fatty acids (SCFAs), which have been shown to protect against various inflammatory diseases.
33708211	3	56	theme	fatty	457:461	arg1	acids					463:467	short-chain fatty acids	445:467	short-chain fatty acids (SCFAs)	445:475	Specifically, bacterial fermentation of dietary fiber produces metabolites called short-chain fatty acids (SCFAs), which have been shown to protect against various inflammatory diseases.
33708211	4	57	theme	fiber	573:577	arg1	deficiency					579:588	fiber deficiency	573:588	fiber deficiency	573:588	However, the effect of fiber deficiency on the key initial steps of inflammation, such as leukocyte-endothelial cell interactions, is unknown.
33708211	5	58	dep	in	807:808	arg1	vivo					810:813	vivo	810:813	vivo	810:813	Moreover, the impact of fiber deficiency on neutrophil recruitment under basal conditions and during inflammation in vivo is unknown.
33708211	3	59	theme	inflammatory	527:538	arg1	diseases					540:547	various inflammatory diseases	519:547	various inflammatory diseases	519:547	Specifically, bacterial fermentation of dietary fiber produces metabolites called short-chain fatty acids (SCFAs), which have been shown to protect against various inflammatory diseases.
33708211	10	60	theme	important	1597:1605	arg1	role					1607:1610	an important role	1594:1610	an important role	1594:1610	Therefore, dietary fiber, possibly through the actions of acetate, plays an important role in regulating neutrophil recruitment and host protection against inflammatory colonic damage in an experimental model of colitis.
33708211	10	61	from	damage	1698:1703	arg1	model					1724:1728	an experimental model	1708:1728	an experimental model of colitis	1708:1739	Therefore, dietary fiber, possibly through the actions of acetate, plays an important role in regulating neutrophil recruitment and host protection against inflammatory colonic damage in an experimental model of colitis.
33708211	6	62	from	state	904:908	arg1	colon					917:921	the colon	913:921	the colon	913:921	Herein, we hypothesized that a fiber-deficient diet promotes an inflammatory state in the colon at baseline and predisposes the host to more severe colitis pathology.
33708211	7	63	from	changes	1050:1056	arg1	microbiota					1069:1078	the gut microbiota	1061:1078	the gut microbiota	1061:1078	Mice fed a no-fiber diet for 14 days showed significant changes in the gut microbiota and exhibited increased neutrophil-endothelial interactions in the colonic microvasculature.
33708211	5	64	theme	fiber	717:721	arg1	deficiency					723:732	fiber deficiency	717:732	fiber deficiency	717:732	Moreover, the impact of fiber deficiency on neutrophil recruitment under basal conditions and during inflammation in vivo is unknown.
33708211	6	65	theme	fiber-deficient	858:872	arg1	diet					874:877	a fiber-deficient diet	856:877	a fiber-deficient diet	856:877	Herein, we hypothesized that a fiber-deficient diet promotes an inflammatory state in the colon at baseline and predisposes the host to more severe colitis pathology.
33708211	9	66	theme	colonic	1478:1484	arg1	neutrophils					1486:1496	colonic neutrophils	1478:1496	colonic neutrophils	1478:1496	Supplementation of the most abundant SCFA, acetate, prevented no-fiber diet-mediated enrichment of colonic neutrophils and colitis pathology.
33708211	9	67	theme	SCFA	1416:1419	arg1	Supplementation					1379:1393	Supplementation	1379:1393	Supplementation of the most abundant SCFA, acetate,	1379:1429	Supplementation of the most abundant SCFA, acetate, prevented no-fiber diet-mediated enrichment of colonic neutrophils and colitis pathology.
33708211	0	68	theme	Neutrophil	66:75	arg1	Recruitment					77:87	Neutrophil Recruitment	66:87	Neutrophil Recruitment	66:87	Deficiency of Dietary Fiber Modulates Gut Microbiota Composition, Neutrophil Recruitment and Worsens Experimental Colitis.
33708211	0	68	theme	Neutrophil	66:75	arg1	Composition					53:63	Gut Microbiota Composition	38:63	Gut Microbiota Composition	38:63	Deficiency of Dietary Fiber Modulates Gut Microbiota Composition, Neutrophil Recruitment and Worsens Experimental Colitis.
33708211	8	69	contain	have	1221:1224	arg1	mice					1182:1185	mice	1182:1185	mice fed a no-fiber diet	1182:1205	Although mice fed a no-fiber diet alone did not have observable colitis-associated symptoms, these animals were highly susceptible to low dose (0.5%) dextran sodium sulphate (DSS)-induced model of colitis.
33708211	8	69	contain	have	1221:1224	arg2	symptoms					1256:1263	observable colitis-associated symptoms	1226:1263	observable colitis-associated symptoms	1226:1263	Although mice fed a no-fiber diet alone did not have observable colitis-associated symptoms, these animals were highly susceptible to low dose (0.5%) dextran sodium sulphate (DSS)-induced model of colitis.
33708211	1	70	theme	inflammatory	148:159	arg1	disease					161:167	an inflammatory disease	145:167	an inflammatory disease of the colon that is associated with colonic neutrophil accumulation	145:236	Ulcerative colitis is an inflammatory disease of the colon that is associated with colonic neutrophil accumulation.
33708211	1	70	theme	inflammatory	148:159	arg1	colitis					134:140	Ulcerative colitis	123:140	Ulcerative colitis	123:140	Ulcerative colitis is an inflammatory disease of the colon that is associated with colonic neutrophil accumulation.
33708211	4	71	theme	leukocyte-endothelial	640:660	arg1	interactions					667:678	leukocyte-endothelial cell interactions	640:678	leukocyte-endothelial cell interactions	640:678	However, the effect of fiber deficiency on the key initial steps of inflammation, such as leukocyte-endothelial cell interactions, is unknown.
33708211	4	72	from	effect	563:568	arg1	steps					609:613	the key initial steps	593:613	the key initial steps	593:613	However, the effect of fiber deficiency on the key initial steps of inflammation, such as leukocyte-endothelial cell interactions, is unknown.
33708211	4	72	from	effect	563:568	arg1	interactions					667:678	leukocyte-endothelial cell interactions	640:678	leukocyte-endothelial cell interactions	640:678	However, the effect of fiber deficiency on the key initial steps of inflammation, such as leukocyte-endothelial cell interactions, is unknown.
33708211	5	73	from	impact	707:712	arg1	recruitment					748:758	neutrophil recruitment	737:758	neutrophil recruitment under basal conditions and during inflammation in vivo	737:813	Moreover, the impact of fiber deficiency on neutrophil recruitment under basal conditions and during inflammation in vivo is unknown.
33708211	9	74	theme	abundant	1407:1414	arg1	acetate					1422:1428	acetate	1422:1428	acetate	1422:1428	Supplementation of the most abundant SCFA, acetate, prevented no-fiber diet-mediated enrichment of colonic neutrophils and colitis pathology.
33708211	9	74	theme	abundant	1407:1414	arg1	SCFA					1416:1419	the most abundant SCFA	1398:1419	the most abundant SCFA	1398:1419	Supplementation of the most abundant SCFA, acetate, prevented no-fiber diet-mediated enrichment of colonic neutrophils and colitis pathology.
33708211	5	75	theme	basal	766:770	arg1	conditions					772:781	basal conditions	766:781	basal conditions	766:781	Moreover, the impact of fiber deficiency on neutrophil recruitment under basal conditions and during inflammation in vivo is unknown.
33295623	7	0	theme	mechanical	1237:1246	arg1	properties					1248:1257	the mechanical properties	1233:1257	the mechanical properties of α-dystroglycan	1233:1275	The conformational effects are relevant to the mechanical properties of α-dystroglycan.
33295623	4	1	theme	modification	713:724	arg1	impact					691:696	the impact	687:696	the impact of the α-O-Man modification on the peptide scaffold	687:748	Comparing the results for an unmodified peptide Ac- PPTTTTKKP-NH2 sequence from α-dystroglycan, and glycoconjugates with either modification on the Ts, we find that the impact of the α-O-Man modification on the peptide scaffold is quite limited, while that of the α-O-GalNAc is more profound.
33295623	0	2	theme	modifications	80:92	arg1	impact					104:109	their impact	98:109	their impact on the mucin-like region of alpha-dystroglycan	98:156	Contrasting the conformational effects of α-O-GalNAc and α-O-Man glycan protein modifications and their impact on the mucin-like region of alpha-dystroglycan.
33295623	0	2	theme	modifications	80:92	arg1	effects					31:37	the conformational effects	12:37	the conformational effects of α-O-GalNAc and α-O-Man glycan protein modifications	12:92	Contrasting the conformational effects of α-O-GalNAc and α-O-Man glycan protein modifications and their impact on the mucin-like region of alpha-dystroglycan.
33295623	1	3	theme	mucin-like	337:346	arg1	region					348:353	the mucin-like region	333:353	the mucin-like region of α-dystroglycan	333:371	We have carried out a comparative study of the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan.
33295623	4	4	theme	α-O-Man	705:711	arg1	modification					713:724	the α-O-Man modification	701:724	the α-O-Man modification	701:724	Comparing the results for an unmodified peptide Ac- PPTTTTKKP-NH2 sequence from α-dystroglycan, and glycoconjugates with either modification on the Ts, we find that the impact of the α-O-Man modification on the peptide scaffold is quite limited, while that of the α-O-GalNAc is more profound.
33295623	1	5	from	context	306:312	arg1	region					348:353	the mucin-like region	333:353	the mucin-like region of α-dystroglycan	333:371	We have carried out a comparative study of the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan.
33295623	1	5	from	context	306:312	arg1	α-O-GalNAc					288:297	α-O-GalNAc	288:297	α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan	288:371	We have carried out a comparative study of the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan.
33295623	1	5	from	context	306:312	arg1	residues					258:265	threonine residues	248:265	threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan	248:371	We have carried out a comparative study of the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan.
33295623	1	5	from	context	306:312	arg1	α-O-Man					277:283	α-O-Man	277:283	α-O-Man	277:283	We have carried out a comparative study of the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan.
33295623	6	6	dep	residue	1087:1093	arg1	appreciated					1120:1130	appreciated	1120:1130	appreciated	1120:1130	Further examination of the NMR-based structure and the MD results suggest a more extensive network of hydrogen bond interactions within the α-O-GalNAc-threonine residue than has been previously appreciated, which influences the properties of the protein backbone.
33295623	6	7	theme	protein	1172:1178	arg1	backbone					1180:1187	the protein backbone	1168:1187	the protein backbone	1168:1187	Further examination of the NMR-based structure and the MD results suggest a more extensive network of hydrogen bond interactions within the α-O-GalNAc-threonine residue than has been previously appreciated, which influences the properties of the protein backbone.
33295623	4	8	from	impact	691:696	arg1	scaffold					741:748	the peptide scaffold	729:748	the peptide scaffold	729:748	Comparing the results for an unmodified peptide Ac- PPTTTTKKP-NH2 sequence from α-dystroglycan, and glycoconjugates with either modification on the Ts, we find that the impact of the α-O-Man modification on the peptide scaffold is quite limited, while that of the α-O-GalNAc is more profound.
33295623	1	9	theme	α-O-Man	277:283	arg1	α-O-GalNAc					288:297	α-O-GalNAc	288:297	α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan	288:371	We have carried out a comparative study of the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan.
33295623	1	9	theme	α-O-Man	277:283	arg1	residues					258:265	threonine residues	248:265	threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan	248:371	We have carried out a comparative study of the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan.
33295623	1	9	theme	α-O-Man	277:283	arg1	α-O-Man					277:283	α-O-Man	277:283	α-O-Man	277:283	We have carried out a comparative study of the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan.
33295623	1	10	theme	α-dystroglycan	358:371	arg1	region					348:353	the mucin-like region	333:353	the mucin-like region of α-dystroglycan	333:371	We have carried out a comparative study of the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan.
33295623	5	11	theme	α-O-GalNAc	835:844	arg1	glycoconjugate					846:859	the α-O-GalNAc glycoconjugate	831:859	the α-O-GalNAc glycoconjugate	831:859	The results for the α-O-GalNAc glycoconjugate are consistent with what has been seen earlier in other systems.
33295623	4	12	from	α-dystroglycan	602:615	arg1	sequence					588:595	an unmodified peptide Ac- PPTTTTKKP-NH2 sequence	548:595	an unmodified peptide Ac- PPTTTTKKP-NH2 sequence from α-dystroglycan	548:615	Comparing the results for an unmodified peptide Ac- PPTTTTKKP-NH2 sequence from α-dystroglycan, and glycoconjugates with either modification on the Ts, we find that the impact of the α-O-Man modification on the peptide scaffold is quite limited, while that of the α-O-GalNAc is more profound.
33295623	4	13	theme	unmodified	551:560	arg1	sequence					588:595	an unmodified peptide Ac- PPTTTTKKP-NH2 sequence	548:595	an unmodified peptide Ac- PPTTTTKKP-NH2 sequence from α-dystroglycan	548:615	Comparing the results for an unmodified peptide Ac- PPTTTTKKP-NH2 sequence from α-dystroglycan, and glycoconjugates with either modification on the Ts, we find that the impact of the α-O-Man modification on the peptide scaffold is quite limited, while that of the α-O-GalNAc is more profound.
33295623	3	14	theme	Solution	446:453	arg1	experiments					459:469	Solution NMR experiments	446:469	Solution NMR experiments	446:469	Solution NMR experiments and molecular dynamics calculations were employed.
33295623	7	15	theme	conformational	1194:1207	arg1	relevant					1221:1228	relevant	1221:1228	relevant	1221:1228	The conformational effects are relevant to the mechanical properties of α-dystroglycan.
33295623	7	15	theme	conformational	1194:1207	arg1	effects					1209:1215	The conformational effects	1190:1215	The conformational effects	1190:1215	The conformational effects are relevant to the mechanical properties of α-dystroglycan.
33295623	6	16	theme	MD	981:982	arg1	results					984:990	the MD results	977:990	the MD results	977:990	Further examination of the NMR-based structure and the MD results suggest a more extensive network of hydrogen bond interactions within the α-O-GalNAc-threonine residue than has been previously appreciated, which influences the properties of the protein backbone.
33295623	6	17	theme	backbone	1180:1187	arg1	properties					1154:1163	the properties	1150:1163	the properties of the protein backbone	1150:1187	Further examination of the NMR-based structure and the MD results suggest a more extensive network of hydrogen bond interactions within the α-O-GalNAc-threonine residue than has been previously appreciated, which influences the properties of the protein backbone.
33295623	1	18	theme	comparative	181:191	arg1	study					193:197	a comparative study	179:197	a comparative study of the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan	179:371	We have carried out a comparative study of the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan.
33295623	1	19	from	residues	258:265	arg1	context					306:312	the context	302:312	the context of a sequence from the mucin-like region of α-dystroglycan	302:371	We have carried out a comparative study of the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan.
33295623	4	20	theme	PPTTTTKKP-NH2	574:586	arg1	sequence					588:595	an unmodified peptide Ac- PPTTTTKKP-NH2 sequence	548:595	an unmodified peptide Ac- PPTTTTKKP-NH2 sequence from α-dystroglycan	548:615	Comparing the results for an unmodified peptide Ac- PPTTTTKKP-NH2 sequence from α-dystroglycan, and glycoconjugates with either modification on the Ts, we find that the impact of the α-O-Man modification on the peptide scaffold is quite limited, while that of the α-O-GalNAc is more profound.
33295623	1	21	from	α-O-GalNAc	288:297	arg1	context					306:312	the context	302:312	the context of a sequence from the mucin-like region of α-dystroglycan	302:371	We have carried out a comparative study of the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan.
33295623	1	22	dep	carried	167:173	arg1	out					175:177	out	175:177	out	175:177	We have carried out a comparative study of the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan.
33295623	2	23	theme	such	379:382	arg1	modifications					384:396	Both such modifications	374:396	Both such modifications	374:396	Both such modifications can coexist in this domain of the glycoprotein.
33295623	2	24	theme	glycoprotein	432:443	arg1	domain					418:423	this domain	413:423	this domain of the glycoprotein	413:443	Both such modifications can coexist in this domain of the glycoprotein.
33295623	2	24	theme	glycoprotein	432:443	arg1	glycoprotein					432:443	the glycoprotein	428:443	the glycoprotein	428:443	Both such modifications can coexist in this domain of the glycoprotein.
33295623	1	25	theme	α-O-GalNAc	288:297	arg1	α-O-GalNAc					288:297	α-O-GalNAc	288:297	α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan	288:371	We have carried out a comparative study of the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan.
33295623	1	25	theme	α-O-GalNAc	288:297	arg1	residues					258:265	threonine residues	248:265	threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan	248:371	We have carried out a comparative study of the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan.
33295623	1	25	theme	α-O-GalNAc	288:297	arg1	α-O-Man					277:283	α-O-Man	277:283	α-O-Man	277:283	We have carried out a comparative study of the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan.
33295623	5	26	theme	other	911:915	arg1	systems					917:923	other systems	911:923	other systems	911:923	The results for the α-O-GalNAc glycoconjugate are consistent with what has been seen earlier in other systems.
33295623	6	27	theme	interactions	1042:1053	arg1	network					1017:1023	a more extensive network	1000:1023	a more extensive network of hydrogen bond interactions within the α-O-GalNAc-threonine residue than has been previously appreciated, which influences the properties of the protein backbone	1000:1187	Further examination of the NMR-based structure and the MD results suggest a more extensive network of hydrogen bond interactions within the α-O-GalNAc-threonine residue than has been previously appreciated, which influences the properties of the protein backbone.
33295623	0	28	theme	conformational	16:29	arg1	effects					31:37	the conformational effects	12:37	the conformational effects of α-O-GalNAc and α-O-Man glycan protein modifications	12:92	Contrasting the conformational effects of α-O-GalNAc and α-O-Man glycan protein modifications and their impact on the mucin-like region of alpha-dystroglycan.
33295623	0	29	theme	mucin-like	118:127	arg1	region					129:134	the mucin-like region	114:134	the mucin-like region of alpha-dystroglycan	114:156	Contrasting the conformational effects of α-O-GalNAc and α-O-Man glycan protein modifications and their impact on the mucin-like region of alpha-dystroglycan.
33295623	3	30	theme	molecular	475:483	arg1	calculations					494:505	molecular dynamics calculations	475:505	molecular dynamics calculations	475:505	Solution NMR experiments and molecular dynamics calculations were employed.
33295623	0	31	theme	α-O-GalNAc	42:51	arg1	modifications					80:92	α-O-GalNAc and α-O-Man glycan protein modifications	42:92	α-O-GalNAc and α-O-Man glycan protein modifications	42:92	Contrasting the conformational effects of α-O-GalNAc and α-O-Man glycan protein modifications and their impact on the mucin-like region of alpha-dystroglycan.
33295623	6	32	theme	structure	963:971	arg1	examination					934:944	Further examination	926:944	Further examination of the NMR-based structure	926:971	Further examination of the NMR-based structure and the MD results suggest a more extensive network of hydrogen bond interactions within the α-O-GalNAc-threonine residue than has been previously appreciated, which influences the properties of the protein backbone.
33295623	6	32	theme	structure	963:971	arg1	results					984:990	the MD results	977:990	the MD results	977:990	Further examination of the NMR-based structure and the MD results suggest a more extensive network of hydrogen bond interactions within the α-O-GalNAc-threonine residue than has been previously appreciated, which influences the properties of the protein backbone.
33295623	0	33	theme	alpha-dystroglycan	139:156	arg1	region					129:134	the mucin-like region	114:134	the mucin-like region of alpha-dystroglycan	114:156	Contrasting the conformational effects of α-O-GalNAc and α-O-Man glycan protein modifications and their impact on the mucin-like region of alpha-dystroglycan.
33295623	3	34	theme	NMR	455:457	arg1	experiments					459:469	Solution NMR experiments	446:469	Solution NMR experiments	446:469	Solution NMR experiments and molecular dynamics calculations were employed.
33295623	6	35	theme	α-O-GalNAc-threonine	1066:1085	arg1	residue					1087:1093	the α-O-GalNAc-threonine residue	1062:1093	the α-O-GalNAc-threonine residue than has been previously appreciated, which influences the properties of the protein backbone	1062:1187	Further examination of the NMR-based structure and the MD results suggest a more extensive network of hydrogen bond interactions within the α-O-GalNAc-threonine residue than has been previously appreciated, which influences the properties of the protein backbone.
33295623	1	36	theme	conformational	206:219	arg1	impact					221:226	the conformational impact	202:226	the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan	202:371	We have carried out a comparative study of the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan.
33295623	1	37	from	region	348:353	arg1	context					306:312	the context	302:312	the context of a sequence from the mucin-like region of α-dystroglycan	302:371	We have carried out a comparative study of the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan.
33295623	1	37	from	region	348:353	arg1	sequence					319:326	a sequence	317:326	a sequence from the mucin-like region of α-dystroglycan	317:371	We have carried out a comparative study of the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan.
33295623	6	38	theme	NMR-based	953:961	arg1	structure					963:971	the NMR-based structure	949:971	the NMR-based structure	949:971	Further examination of the NMR-based structure and the MD results suggest a more extensive network of hydrogen bond interactions within the α-O-GalNAc-threonine residue than has been previously appreciated, which influences the properties of the protein backbone.
33295623	6	39	theme	extensive	1007:1015	arg1	network					1017:1023	a more extensive network	1000:1023	a more extensive network of hydrogen bond interactions within the α-O-GalNAc-threonine residue than has been previously appreciated, which influences the properties of the protein backbone	1000:1187	Further examination of the NMR-based structure and the MD results suggest a more extensive network of hydrogen bond interactions within the α-O-GalNAc-threonine residue than has been previously appreciated, which influences the properties of the protein backbone.
33295623	1	40	theme	impact	221:226	arg1	study					193:197	a comparative study	179:197	a comparative study of the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan	179:371	We have carried out a comparative study of the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan.
33295623	0	41	theme	α-O-Man	57:63	arg1	modifications					80:92	α-O-GalNAc and α-O-Man glycan protein modifications	42:92	α-O-GalNAc and α-O-Man glycan protein modifications	42:92	Contrasting the conformational effects of α-O-GalNAc and α-O-Man glycan protein modifications and their impact on the mucin-like region of alpha-dystroglycan.
33295623	4	42	theme	Ac-	570:572	arg1	sequence					588:595	an unmodified peptide Ac- PPTTTTKKP-NH2 sequence	548:595	an unmodified peptide Ac- PPTTTTKKP-NH2 sequence from α-dystroglycan	548:615	Comparing the results for an unmodified peptide Ac- PPTTTTKKP-NH2 sequence from α-dystroglycan, and glycoconjugates with either modification on the Ts, we find that the impact of the α-O-Man modification on the peptide scaffold is quite limited, while that of the α-O-GalNAc is more profound.
33295623	4	43	with	results	536:542	arg1	modification					650:661	either modification	643:661	either modification	643:661	Comparing the results for an unmodified peptide Ac- PPTTTTKKP-NH2 sequence from α-dystroglycan, and glycoconjugates with either modification on the Ts, we find that the impact of the α-O-Man modification on the peptide scaffold is quite limited, while that of the α-O-GalNAc is more profound.
33295623	4	44	theme	peptide	562:568	arg1	sequence					588:595	an unmodified peptide Ac- PPTTTTKKP-NH2 sequence	548:595	an unmodified peptide Ac- PPTTTTKKP-NH2 sequence from α-dystroglycan	548:615	Comparing the results for an unmodified peptide Ac- PPTTTTKKP-NH2 sequence from α-dystroglycan, and glycoconjugates with either modification on the Ts, we find that the impact of the α-O-Man modification on the peptide scaffold is quite limited, while that of the α-O-GalNAc is more profound.
33295623	6	45	theme	Further	926:932	arg1	examination					934:944	Further examination	926:944	Further examination of the NMR-based structure	926:971	Further examination of the NMR-based structure and the MD results suggest a more extensive network of hydrogen bond interactions within the α-O-GalNAc-threonine residue than has been previously appreciated, which influences the properties of the protein backbone.
33295623	1	46	theme	sequence	319:326	arg1	context					306:312	the context	302:312	the context of a sequence from the mucin-like region of α-dystroglycan	302:371	We have carried out a comparative study of the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan.
33295623	4	47	with	glycoconjugates	622:636	arg1	modification					650:661	either modification	643:661	either modification	643:661	Comparing the results for an unmodified peptide Ac- PPTTTTKKP-NH2 sequence from α-dystroglycan, and glycoconjugates with either modification on the Ts, we find that the impact of the α-O-Man modification on the peptide scaffold is quite limited, while that of the α-O-GalNAc is more profound.
33295623	3	48	theme	dynamics	485:492	arg1	calculations					494:505	molecular dynamics calculations	475:505	molecular dynamics calculations	475:505	Solution NMR experiments and molecular dynamics calculations were employed.
33295623	6	49	theme	hydrogen	1028:1035	arg1	interactions					1042:1053	hydrogen bond interactions	1028:1053	hydrogen bond interactions within the α-O-GalNAc-threonine residue than has been previously appreciated, which influences the properties of the protein backbone	1028:1187	Further examination of the NMR-based structure and the MD results suggest a more extensive network of hydrogen bond interactions within the α-O-GalNAc-threonine residue than has been previously appreciated, which influences the properties of the protein backbone.
33295623	0	50	from	impact	104:109	arg1	region					129:134	the mucin-like region	114:134	the mucin-like region of alpha-dystroglycan	114:156	Contrasting the conformational effects of α-O-GalNAc and α-O-Man glycan protein modifications and their impact on the mucin-like region of alpha-dystroglycan.
33295623	4	51	theme	peptide	733:739	arg1	scaffold					741:748	the peptide scaffold	729:748	the peptide scaffold	729:748	Comparing the results for an unmodified peptide Ac- PPTTTTKKP-NH2 sequence from α-dystroglycan, and glycoconjugates with either modification on the Ts, we find that the impact of the α-O-Man modification on the peptide scaffold is quite limited, while that of the α-O-GalNAc is more profound.
33295623	1	52	theme	modifications	231:243	arg1	impact					221:226	the conformational impact	202:226	the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan	202:371	We have carried out a comparative study of the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan.
33295623	0	53	theme	protein	72:78	arg1	modifications					80:92	α-O-GalNAc and α-O-Man glycan protein modifications	42:92	α-O-GalNAc and α-O-Man glycan protein modifications	42:92	Contrasting the conformational effects of α-O-GalNAc and α-O-Man glycan protein modifications and their impact on the mucin-like region of alpha-dystroglycan.
33295623	6	54	theme	bond	1037:1040	arg1	interactions					1042:1053	hydrogen bond interactions	1028:1053	hydrogen bond interactions within the α-O-GalNAc-threonine residue than has been previously appreciated, which influences the properties of the protein backbone	1028:1187	Further examination of the NMR-based structure and the MD results suggest a more extensive network of hydrogen bond interactions within the α-O-GalNAc-threonine residue than has been previously appreciated, which influences the properties of the protein backbone.
33295623	1	55	from	α-O-Man	277:283	arg1	context					306:312	the context	302:312	the context of a sequence from the mucin-like region of α-dystroglycan	302:371	We have carried out a comparative study of the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan.
33295623	7	56	theme	α-dystroglycan	1262:1275	arg1	properties					1248:1257	the mechanical properties	1233:1257	the mechanical properties of α-dystroglycan	1233:1275	The conformational effects are relevant to the mechanical properties of α-dystroglycan.
33295623	0	57	theme	glycan	65:70	arg1	modifications					80:92	α-O-GalNAc and α-O-Man glycan protein modifications	42:92	α-O-GalNAc and α-O-Man glycan protein modifications	42:92	Contrasting the conformational effects of α-O-GalNAc and α-O-Man glycan protein modifications and their impact on the mucin-like region of alpha-dystroglycan.
33295623	0	58	from	effects	31:37	arg1	region					129:134	the mucin-like region	114:134	the mucin-like region of alpha-dystroglycan	114:156	Contrasting the conformational effects of α-O-GalNAc and α-O-Man glycan protein modifications and their impact on the mucin-like region of alpha-dystroglycan.
33295623	2	59	gly	glycoprotein	432:443	arg1	glycoprotein					432:443	the glycoprotein	428:443	the glycoprotein	428:443	Both such modifications can coexist in this domain of the glycoprotein.
33295623	1	60	theme	threonine	248:256	arg1	α-O-GalNAc					288:297	α-O-GalNAc	288:297	α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan	288:371	We have carried out a comparative study of the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan.
33295623	1	60	theme	threonine	248:256	arg1	residues					258:265	threonine residues	248:265	threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan	248:371	We have carried out a comparative study of the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan.
33295623	1	60	theme	threonine	248:256	arg1	α-O-Man					277:283	α-O-Man	277:283	α-O-Man	277:283	We have carried out a comparative study of the conformational impact of modifications to threonine residues of either α-O-Man or α-O-GalNAc in the context of a sequence from the mucin-like region of α-dystroglycan.
33104820	8	0	theme	relatedness	992:1002	arg1	%					1125:1125	21.2%	1121:1125	21.2%	1121:1125	The DNA‒DNA relatedness (DDH) values of isolate Hz 6-5T with N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T were 21.2%, 36.5%, and 32.0%, respectively.
33104820	8	0	theme	relatedness	992:1002	arg1	values					1010:1015	The DNA‒DNA relatedness (DDH) values	980:1015	The DNA‒DNA relatedness (DDH) values of isolate Hz 6-5T with N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T	980:1114	The DNA‒DNA relatedness (DDH) values of isolate Hz 6-5T with N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T were 21.2%, 36.5%, and 32.0%, respectively.
33104820	4	1	theme	KCl	506:508	arg1	presence					466:473	the presence	462:473	the presence of 0.5% (w/v) NaCl or 3% (w/v) KCl	462:508	The strain was aerobic with optimal growth at 33 °C, pH 9.0, and in the presence of 0.5% (w/v) NaCl or 3% (w/v) KCl.
33104820	9	2	theme	strain	1195:1200	arg1	6-5T					1205:1208	strain Hz 6-5T	1195:1208	strain Hz 6-5T	1195:1208	The characteristic diamino acid of strain Hz 6-5T was found to be lysine.
33104820	8	3	theme	DDH	1005:1007	arg1	%					1125:1125	21.2%	1121:1125	21.2%	1121:1125	The DNA‒DNA relatedness (DDH) values of isolate Hz 6-5T with N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T were 21.2%, 36.5%, and 32.0%, respectively.
33104820	8	3	theme	DDH	1005:1007	arg1	values					1010:1015	The DNA‒DNA relatedness (DDH) values	980:1015	The DNA‒DNA relatedness (DDH) values of isolate Hz 6-5T with N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T	980:1114	The DNA‒DNA relatedness (DDH) values of isolate Hz 6-5T with N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T were 21.2%, 36.5%, and 32.0%, respectively.
33104820	13	4	theme	physiological	1725:1737	arg1	evidence					1793:1800	morphological, physiological, chemotaxonomic, and other differentiating phenotypic evidence	1710:1800	morphological, physiological, chemotaxonomic, and other differentiating phenotypic evidence	1710:1800	Based on the results of phylogenetic analysis supported by morphological, physiological, chemotaxonomic, and other differentiating phenotypic evidence, strain Hz 6-5T is considered to represent a novel species of the genus Nesterenkonia, for which the name Nesterenkonia haasae sp.
33104820	13	5	theme	analysis	1688:1695	arg1	results					1664:1670	the results	1660:1670	the results of phylogenetic analysis supported by morphological, physiological, chemotaxonomic, and other differentiating phenotypic evidence	1660:1800	Based on the results of phylogenetic analysis supported by morphological, physiological, chemotaxonomic, and other differentiating phenotypic evidence, strain Hz 6-5T is considered to represent a novel species of the genus Nesterenkonia, for which the name Nesterenkonia haasae sp.
33104820	9	6	theme	6-5T	1205:1208	arg1	acid					1187:1190	The characteristic diamino acid	1160:1190	The characteristic diamino acid of strain Hz 6-5T	1160:1208	The characteristic diamino acid of strain Hz 6-5T was found to be lysine.
33104820	9	6	theme	6-5T	1205:1208	arg1	lysine					1226:1231	lysine	1226:1231	lysine	1226:1231	The characteristic diamino acid of strain Hz 6-5T was found to be lysine.
33104820	8	7	theme	N.	1062:1063	arg1	1.2323T					1108:1114	N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T	1041:1114	N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T	1041:1114	The DNA‒DNA relatedness (DDH) values of isolate Hz 6-5T with N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T were 21.2%, 36.5%, and 32.0%, respectively.
33104820	8	8	theme	isolate	1020:1026	arg1	6-5T					1031:1034	isolate Hz 6-5T	1020:1034	isolate Hz 6-5T	1020:1034	The DNA‒DNA relatedness (DDH) values of isolate Hz 6-5T with N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T were 21.2%, 36.5%, and 32.0%, respectively.
33104820	8	9	theme	DSM	1075:1077	arg1	16378T					1079:1084	DSM 16378T	1075:1084	N. halophila DSM 16378T	1062:1084	The DNA‒DNA relatedness (DDH) values of isolate Hz 6-5T with N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T were 21.2%, 36.5%, and 32.0%, respectively.
33104820	1	10	attach	isolated	63:70	arg2	actinobacterium					47:61	an alkaliphilic actinobacterium	31:61	an alkaliphilic actinobacterium isolated from a degraded pasture in Songnen Plain	31:111	nov., an alkaliphilic actinobacterium isolated from a degraded pasture in Songnen Plain.
33104820	1	10	attach	isolated	63:70	arg1	pasture					88:94	a degraded pasture	77:94	a degraded pasture in Songnen Plain	77:111	nov., an alkaliphilic actinobacterium isolated from a degraded pasture in Songnen Plain.
33104820	7	11	theme	phylogenomic	928:939	arg1	analysis					941:948	the phylogenomic analysis	924:948	the phylogenomic analysis	924:948	The phylogenetic tree based on the phylogenomic analysis also showed the same results.
33104820	8	12	theme	6-5T	1031:1034	arg1	%					1125:1125	21.2%	1121:1125	21.2%	1121:1125	The DNA‒DNA relatedness (DDH) values of isolate Hz 6-5T with N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T were 21.2%, 36.5%, and 32.0%, respectively.
33104820	8	12	theme	6-5T	1031:1034	arg1	values					1010:1015	The DNA‒DNA relatedness (DDH) values	980:1015	The DNA‒DNA relatedness (DDH) values of isolate Hz 6-5T with N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T	980:1114	The DNA‒DNA relatedness (DDH) values of isolate Hz 6-5T with N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T were 21.2%, 36.5%, and 32.0%, respectively.
33104820	6	13	theme	species	818:824	arg1	subclade					778:785	a subclade	776:785	a subclade with the type strains of these species	776:824	The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences.
33104820	3	14	theme	light	275:279	arg1	colonies					296:303	light yellow-colored colonies	275:303	light yellow-colored colonies	275:303	The isolate formed light yellow-colored colonies and its cells were Gram-staining positive, non-motile, and non-spore-forming short rods.
33104820	8	15	theme	N.	1091:1092	arg1	1.2323T					1108:1114	N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T	1041:1114	N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T	1041:1114	The DNA‒DNA relatedness (DDH) values of isolate Hz 6-5T with N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T were 21.2%, 36.5%, and 32.0%, respectively.
33104820	13	16	theme	genus	1868:1872	arg1	Nesterenkonia					1874:1886	the genus Nesterenkonia	1864:1886	the genus Nesterenkonia	1864:1886	Based on the results of phylogenetic analysis supported by morphological, physiological, chemotaxonomic, and other differentiating phenotypic evidence, strain Hz 6-5T is considered to represent a novel species of the genus Nesterenkonia, for which the name Nesterenkonia haasae sp.
33104820	8	17	theme	M8T	1057:1059	arg1	1.2323T					1108:1114	N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T	1041:1114	N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T	1041:1114	The DNA‒DNA relatedness (DDH) values of isolate Hz 6-5T with N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T were 21.2%, 36.5%, and 32.0%, respectively.
33104820	6	18	theme	type	796:799	arg1	strains					801:807	the type strains	792:807	the type strains	792:807	The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences.
33104820	8	19	theme	CGMCC	1102:1106	arg1	1.2323T					1108:1114	N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T	1041:1114	N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T	1041:1114	The DNA‒DNA relatedness (DDH) values of isolate Hz 6-5T with N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T were 21.2%, 36.5%, and 32.0%, respectively.
33104820	10	20	theme	cellular	1318:1325	arg1	acids					1333:1337	the major cellular fatty acids	1308:1337	the major cellular fatty acids (> 10%)	1308:1345	The respiratory quinones were MK-9, MK-8, MK-7(H4), MK-7(H2) and MK-7 and the major cellular fatty acids (> 10%) were anteiso-C15:0, anteiso-C17:0 and iso-C16:0.
33104820	10	20	theme	cellular	1318:1325	arg1	%					1344:1344	> 10%	1340:1344	> 10%	1340:1344	The respiratory quinones were MK-9, MK-8, MK-7(H4), MK-7(H2) and MK-7 and the major cellular fatty acids (> 10%) were anteiso-C15:0, anteiso-C17:0 and iso-C16:0.
33104820	10	20	theme	cellular	1318:1325	arg1	anteiso-C15:0					1352:1364	anteiso-C15:0	1352:1364	anteiso-C15:0	1352:1364	The respiratory quinones were MK-9, MK-8, MK-7(H4), MK-7(H2) and MK-7 and the major cellular fatty acids (> 10%) were anteiso-C15:0, anteiso-C17:0 and iso-C16:0.
33104820	11	21	theme	unidentified	1538:1549	arg1	glycolipid					1551:1560	an unidentified glycolipid	1535:1560	an unidentified glycolipid	1535:1560	The polar lipids detected for strain Hz 6-5T were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol, an unidentified glycolipid, and two unidentified phospholipids.
33104820	11	21	theme	unidentified	1538:1549	arg1	diphosphatidylglycerol					1446:1467	diphosphatidylglycerol	1446:1467	diphosphatidylglycerol	1446:1467	The polar lipids detected for strain Hz 6-5T were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol, an unidentified glycolipid, and two unidentified phospholipids.
33104820	4	22	theme	optimal	422:428	arg1	growth					430:435	optimal growth	422:435	optimal growth at 33 °C, pH 9.0,	422:453	The strain was aerobic with optimal growth at 33 °C, pH 9.0, and in the presence of 0.5% (w/v) NaCl or 3% (w/v) KCl.
33104820	1	23	dep	actinobacterium	47:61	arg1	nov.					25:28	nov.	25:28	nov.	25:28	nov., an alkaliphilic actinobacterium isolated from a degraded pasture in Songnen Plain.
33104820	6	24	theme	rRNA	586:589	arg1	similarities					605:616	highest 16S rRNA gene sequence similarities	574:616	highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T	574:742	The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences.
33104820	6	25	theme	16S	868:870	arg1	sequences					882:890	the 16S rRNA gene sequences	864:890	the 16S rRNA gene sequences	864:890	The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences.
33104820	3	26	theme	positive	338:345	arg1	rods					388:391	Gram-staining positive, non-motile, and non-spore-forming short rods	324:391	Gram-staining positive, non-motile, and non-spore-forming short rods	324:391	The isolate formed light yellow-colored colonies and its cells were Gram-staining positive, non-motile, and non-spore-forming short rods.
33104820	6	27	theme	highest	574:580	arg1	similarities					605:616	highest 16S rRNA gene sequence similarities	574:616	highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T	574:742	The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences.
33104820	11	28	theme	Hz	1433:1434	arg1	6-5T					1436:1439	strain Hz 6-5T	1426:1439	strain Hz 6-5T	1426:1439	The polar lipids detected for strain Hz 6-5T were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol, an unidentified glycolipid, and two unidentified phospholipids.
33104820	13	29	theme	Nesterenkonia	1874:1886	arg1	species					1853:1859	a novel species	1845:1859	a novel species	1845:1859	Based on the results of phylogenetic analysis supported by morphological, physiological, chemotaxonomic, and other differentiating phenotypic evidence, strain Hz 6-5T is considered to represent a novel species of the genus Nesterenkonia, for which the name Nesterenkonia haasae sp.
33104820	13	30	theme	Hz	1810:1811	arg1	6-5T					1813:1816	strain Hz 6-5T	1803:1816	strain Hz 6-5T	1803:1816	Based on the results of phylogenetic analysis supported by morphological, physiological, chemotaxonomic, and other differentiating phenotypic evidence, strain Hz 6-5T is considered to represent a novel species of the genus Nesterenkonia, for which the name Nesterenkonia haasae sp.
33104820	4	31	theme	%	481:481	arg1	NaCl					489:492	0.5% (w/v) NaCl	478:492	0.5% (w/v) NaCl	478:492	The strain was aerobic with optimal growth at 33 °C, pH 9.0, and in the presence of 0.5% (w/v) NaCl or 3% (w/v) KCl.
33104820	13	32	theme	other	1760:1764	arg1	evidence					1793:1800	morphological, physiological, chemotaxonomic, and other differentiating phenotypic evidence	1710:1800	morphological, physiological, chemotaxonomic, and other differentiating phenotypic evidence	1710:1800	Based on the results of phylogenetic analysis supported by morphological, physiological, chemotaxonomic, and other differentiating phenotypic evidence, strain Hz 6-5T is considered to represent a novel species of the genus Nesterenkonia, for which the name Nesterenkonia haasae sp.
33104820	3	33	theme	non-motile	348:357	arg1	rods					388:391	Gram-staining positive, non-motile, and non-spore-forming short rods	324:391	Gram-staining positive, non-motile, and non-spore-forming short rods	324:391	The isolate formed light yellow-colored colonies and its cells were Gram-staining positive, non-motile, and non-spore-forming short rods.
33104820	10	34	theme	respiratory	1238:1248	arg1	MK-9					1264:1267	MK-9	1264:1267	MK-9	1264:1267	The respiratory quinones were MK-9, MK-8, MK-7(H4), MK-7(H2) and MK-7 and the major cellular fatty acids (> 10%) were anteiso-C15:0, anteiso-C17:0 and iso-C16:0.
33104820	10	34	theme	respiratory	1238:1248	arg1	quinones					1250:1257	The respiratory quinones	1234:1257	The respiratory quinones	1234:1257	The respiratory quinones were MK-9, MK-8, MK-7(H4), MK-7(H2) and MK-7 and the major cellular fatty acids (> 10%) were anteiso-C15:0, anteiso-C17:0 and iso-C16:0.
33104820	4	35	dep	%	498:498	arg1	w/v					501:503	w/v	501:503	w/v	501:503	The strain was aerobic with optimal growth at 33 °C, pH 9.0, and in the presence of 0.5% (w/v) NaCl or 3% (w/v) KCl.
33104820	6	36	theme	type	625:628	arg1	strains					630:636	the type strains	621:636	the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T	621:742	The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences.
33104820	6	36	theme	type	625:628	arg1	M8T					679:681	M8T	679:681	M8T	679:681	The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences.
33104820	6	36	theme	type	625:628	arg1	GY074T					704:709	GY074T	704:709	GY074T	704:709	The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences.
33104820	6	36	theme	type	625:628	arg1	GY239T					737:742	GY239T	737:742	GY239T	737:742	The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences.
33104820	0	37	theme	Nesterenkonia	0:12	arg1	sp					21:22	Nesterenkonia haasae sp	0:22	Nesterenkonia haasae sp.	0:23	Nesterenkonia haasae sp.
33104820	12	38	theme	Hz	1632:1633	arg1	6-5T					1635:1638	isolate Hz 6-5T	1624:1638	isolate Hz 6-5T	1624:1638	The DNA G + C content of isolate Hz 6-5T was 60.8%.
33104820	3	39	theme	short	382:386	arg1	rods					388:391	Gram-staining positive, non-motile, and non-spore-forming short rods	324:391	Gram-staining positive, non-motile, and non-spore-forming short rods	324:391	The isolate formed light yellow-colored colonies and its cells were Gram-staining positive, non-motile, and non-spore-forming short rods.
33104820	4	40	theme	3	497:497	arg1	%					498:498	%	498:498	%	498:498	The strain was aerobic with optimal growth at 33 °C, pH 9.0, and in the presence of 0.5% (w/v) NaCl or 3% (w/v) KCl.
33104820	15	41	theme	Hz	1970:1971	arg1	strain					1960:1965	The type strain	1951:1965	The type strain	1951:1965	The type strain is Hz 6-5T (=CPCC 205100T=NBRC 113521T).
33104820	15	41	theme	Hz	1970:1971	arg1	6-5T					1973:1976	Hz 6-5T	1970:1976	Hz 6-5T (=CPCC 205100T=NBRC 113521T)	1970:2005	The type strain is Hz 6-5T (=CPCC 205100T=NBRC 113521T).
33104820	15	41	theme	Hz	1970:1971	arg1	113521T					1998:2004	=CPCC 205100T=NBRC 113521T	1979:2004	=CPCC 205100T=NBRC 113521T	1979:2004	The type strain is Hz 6-5T (=CPCC 205100T=NBRC 113521T).
33104820	1	42	theme	Songnen	99:105	arg1	Plain					107:111	Songnen Plain	99:111	Songnen Plain	99:111	nov., an alkaliphilic actinobacterium isolated from a degraded pasture in Songnen Plain.
33104820	11	43	theme	polar	1400:1404	arg1	diphosphatidylglycerol					1446:1467	diphosphatidylglycerol	1446:1467	diphosphatidylglycerol	1446:1467	The polar lipids detected for strain Hz 6-5T were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol, an unidentified glycolipid, and two unidentified phospholipids.
33104820	11	43	theme	polar	1400:1404	arg1	lipids					1406:1411	The polar lipids	1396:1411	The polar lipids detected for strain Hz 6-5T	1396:1439	The polar lipids detected for strain Hz 6-5T were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol, an unidentified glycolipid, and two unidentified phospholipids.
33104820	6	44	theme	sequence	596:603	arg1	similarities					605:616	highest 16S rRNA gene sequence similarities	574:616	highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T	574:742	The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences.
33104820	13	45	theme	Nesterenkonia	1908:1920	arg1	sp					1929:1930	the name Nesterenkonia haasae sp	1899:1930	the name Nesterenkonia haasae sp	1899:1930	Based on the results of phylogenetic analysis supported by morphological, physiological, chemotaxonomic, and other differentiating phenotypic evidence, strain Hz 6-5T is considered to represent a novel species of the genus Nesterenkonia, for which the name Nesterenkonia haasae sp.
33104820	12	46	theme	G + C	1607:1611	arg1	%					1648:1648	60.8%	1644:1648	60.8%	1644:1648	The DNA G + C content of isolate Hz 6-5T was 60.8%.
33104820	12	46	theme	G + C	1607:1611	arg1	content					1613:1619	The DNA G + C content	1599:1619	The DNA G + C content of isolate Hz 6-5T	1599:1638	The DNA G + C content of isolate Hz 6-5T was 60.8%.
33104820	6	47	theme	gene	877:880	arg1	sequences					882:890	the 16S rRNA gene sequences	864:890	the 16S rRNA gene sequences	864:890	The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences.
33104820	2	48	theme	Hz	165:166	arg1	6-5T					168:171	Hz 6-5T	165:171	Hz 6-5T	165:171	An alkaliphilic actinobacterial strain, designated Hz 6-5T, was isolated from saline-alkaline soil from Songnen Plain in north-eastern China.
33104820	13	49	theme	chemotaxonomic	1740:1753	arg1	evidence					1793:1800	morphological, physiological, chemotaxonomic, and other differentiating phenotypic evidence	1710:1800	morphological, physiological, chemotaxonomic, and other differentiating phenotypic evidence	1710:1800	Based on the results of phylogenetic analysis supported by morphological, physiological, chemotaxonomic, and other differentiating phenotypic evidence, strain Hz 6-5T is considered to represent a novel species of the genus Nesterenkonia, for which the name Nesterenkonia haasae sp.
33104820	4	50	theme	NaCl	489:492	arg1	presence					466:473	the presence	462:473	the presence of 0.5% (w/v) NaCl or 3% (w/v) KCl	462:508	The strain was aerobic with optimal growth at 33 °C, pH 9.0, and in the presence of 0.5% (w/v) NaCl or 3% (w/v) KCl.
33104820	11	51	theme	strain	1426:1431	arg1	6-5T					1436:1439	strain Hz 6-5T	1426:1439	strain Hz 6-5T	1426:1439	The polar lipids detected for strain Hz 6-5T were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol, an unidentified glycolipid, and two unidentified phospholipids.
33104820	13	52	theme	morphological	1710:1722	arg1	evidence					1793:1800	morphological, physiological, chemotaxonomic, and other differentiating phenotypic evidence	1710:1800	morphological, physiological, chemotaxonomic, and other differentiating phenotypic evidence	1710:1800	Based on the results of phylogenetic analysis supported by morphological, physiological, chemotaxonomic, and other differentiating phenotypic evidence, strain Hz 6-5T is considered to represent a novel species of the genus Nesterenkonia, for which the name Nesterenkonia haasae sp.
33104820	1	53	from	pasture	88:94	arg1	Plain					107:111	Songnen Plain	99:111	Songnen Plain	99:111	nov., an alkaliphilic actinobacterium isolated from a degraded pasture in Songnen Plain.
33104820	9	54	theme	diamino	1179:1185	arg1	acid					1187:1190	The characteristic diamino acid	1160:1190	The characteristic diamino acid of strain Hz 6-5T	1160:1208	The characteristic diamino acid of strain Hz 6-5T was found to be lysine.
33104820	9	54	theme	diamino	1179:1185	arg1	lysine					1226:1231	lysine	1226:1231	lysine	1226:1231	The characteristic diamino acid of strain Hz 6-5T was found to be lysine.
33104820	1	55	theme	alkaliphilic	34:45	arg1	actinobacterium					47:61	an alkaliphilic actinobacterium	31:61	an alkaliphilic actinobacterium isolated from a degraded pasture in Songnen Plain	31:111	nov., an alkaliphilic actinobacterium isolated from a degraded pasture in Songnen Plain.
33104820	13	56	theme	haasae	1922:1927	arg1	sp					1929:1930	the name Nesterenkonia haasae sp	1899:1930	the name Nesterenkonia haasae sp	1899:1930	Based on the results of phylogenetic analysis supported by morphological, physiological, chemotaxonomic, and other differentiating phenotypic evidence, strain Hz 6-5T is considered to represent a novel species of the genus Nesterenkonia, for which the name Nesterenkonia haasae sp.
33104820	7	57	theme	same	966:969	arg1	results					971:977	the same results	962:977	the same results	962:977	The phylogenetic tree based on the phylogenomic analysis also showed the same results.
33104820	6	58	theme	species	645:651	arg1	strains					630:636	the type strains	621:636	the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T	621:742	The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences.
33104820	6	58	theme	species	645:651	arg1	M8T					679:681	M8T	679:681	M8T	679:681	The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences.
33104820	6	58	theme	species	645:651	arg1	GY074T					704:709	GY074T	704:709	GY074T	704:709	The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences.
33104820	6	58	theme	species	645:651	arg1	GY239T					737:742	GY239T	737:742	GY239T	737:742	The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences.
33104820	8	59	theme	DNA‒DNA	984:990	arg1	%					1125:1125	21.2%	1121:1125	21.2%	1121:1125	The DNA‒DNA relatedness (DDH) values of isolate Hz 6-5T with N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T were 21.2%, 36.5%, and 32.0%, respectively.
33104820	8	59	theme	DNA‒DNA	984:990	arg1	values					1010:1015	The DNA‒DNA relatedness (DDH) values	980:1015	The DNA‒DNA relatedness (DDH) values of isolate Hz 6-5T with N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T	980:1114	The DNA‒DNA relatedness (DDH) values of isolate Hz 6-5T with N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T were 21.2%, 36.5%, and 32.0%, respectively.
33104820	13	60	theme	phylogenetic	1675:1686	arg1	analysis					1688:1695	phylogenetic analysis	1675:1695	phylogenetic analysis supported by morphological, physiological, chemotaxonomic, and other differentiating phenotypic evidence	1675:1800	Based on the results of phylogenetic analysis supported by morphological, physiological, chemotaxonomic, and other differentiating phenotypic evidence, strain Hz 6-5T is considered to represent a novel species of the genus Nesterenkonia, for which the name Nesterenkonia haasae sp.
33104820	9	61	theme	Hz	1202:1203	arg1	6-5T					1205:1208	strain Hz 6-5T	1195:1208	strain Hz 6-5T	1195:1208	The characteristic diamino acid of strain Hz 6-5T was found to be lysine.
33104820	10	62	theme	major	1312:1316	arg1	acids					1333:1337	the major cellular fatty acids	1308:1337	the major cellular fatty acids (> 10%)	1308:1345	The respiratory quinones were MK-9, MK-8, MK-7(H4), MK-7(H2) and MK-7 and the major cellular fatty acids (> 10%) were anteiso-C15:0, anteiso-C17:0 and iso-C16:0.
33104820	10	62	theme	major	1312:1316	arg1	%					1344:1344	> 10%	1340:1344	> 10%	1340:1344	The respiratory quinones were MK-9, MK-8, MK-7(H4), MK-7(H2) and MK-7 and the major cellular fatty acids (> 10%) were anteiso-C15:0, anteiso-C17:0 and iso-C16:0.
33104820	10	62	theme	major	1312:1316	arg1	anteiso-C15:0					1352:1364	anteiso-C15:0	1352:1364	anteiso-C15:0	1352:1364	The respiratory quinones were MK-9, MK-8, MK-7(H4), MK-7(H2) and MK-7 and the major cellular fatty acids (> 10%) were anteiso-C15:0, anteiso-C17:0 and iso-C16:0.
33104820	2	63	theme	actinobacterial	130:144	arg1	strain					146:151	An alkaliphilic actinobacterial strain	114:151	An alkaliphilic actinobacterial strain	114:151	An alkaliphilic actinobacterial strain, designated Hz 6-5T, was isolated from saline-alkaline soil from Songnen Plain in north-eastern China.
33104820	8	64	theme	Hz	1028:1029	arg1	6-5T					1031:1034	isolate Hz 6-5T	1020:1034	isolate Hz 6-5T	1020:1034	The DNA‒DNA relatedness (DDH) values of isolate Hz 6-5T with N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T were 21.2%, 36.5%, and 32.0%, respectively.
33104820	3	65	theme	yellow-colored	281:294	arg1	colonies					296:303	light yellow-colored colonies	275:303	light yellow-colored colonies	275:303	The isolate formed light yellow-colored colonies and its cells were Gram-staining positive, non-motile, and non-spore-forming short rods.
33104820	7	66	theme	phylogenetic	897:908	arg1	tree					910:913	The phylogenetic tree	893:913	The phylogenetic tree based on the phylogenomic analysis	893:948	The phylogenetic tree based on the phylogenomic analysis also showed the same results.
33104820	13	67	theme	novel	1847:1851	arg1	species					1853:1859	a novel species	1845:1859	a novel species	1845:1859	Based on the results of phylogenetic analysis supported by morphological, physiological, chemotaxonomic, and other differentiating phenotypic evidence, strain Hz 6-5T is considered to represent a novel species of the genus Nesterenkonia, for which the name Nesterenkonia haasae sp.
33104820	10	68	theme	fatty	1327:1331	arg1	acids					1333:1337	the major cellular fatty acids	1308:1337	the major cellular fatty acids (> 10%)	1308:1345	The respiratory quinones were MK-9, MK-8, MK-7(H4), MK-7(H2) and MK-7 and the major cellular fatty acids (> 10%) were anteiso-C15:0, anteiso-C17:0 and iso-C16:0.
33104820	10	68	theme	fatty	1327:1331	arg1	%					1344:1344	> 10%	1340:1344	> 10%	1340:1344	The respiratory quinones were MK-9, MK-8, MK-7(H4), MK-7(H2) and MK-7 and the major cellular fatty acids (> 10%) were anteiso-C15:0, anteiso-C17:0 and iso-C16:0.
33104820	10	68	theme	fatty	1327:1331	arg1	anteiso-C15:0					1352:1364	anteiso-C15:0	1352:1364	anteiso-C15:0	1352:1364	The respiratory quinones were MK-9, MK-8, MK-7(H4), MK-7(H2) and MK-7 and the major cellular fatty acids (> 10%) were anteiso-C15:0, anteiso-C17:0 and iso-C16:0.
33104820	4	69	from	33 °C	440:444	arg1	growth					430:435	optimal growth	422:435	optimal growth at 33 °C, pH 9.0,	422:453	The strain was aerobic with optimal growth at 33 °C, pH 9.0, and in the presence of 0.5% (w/v) NaCl or 3% (w/v) KCl.
33104820	2	70	theme	Songnen	218:224	arg1	Plain					226:230	Songnen Plain	218:230	Songnen Plain in north-eastern China	218:253	An alkaliphilic actinobacterial strain, designated Hz 6-5T, was isolated from saline-alkaline soil from Songnen Plain in north-eastern China.
33104820	2	71	theme	saline-alkaline	192:206	arg1	soil					208:211	saline-alkaline soil	192:211	saline-alkaline soil	192:211	An alkaliphilic actinobacterial strain, designated Hz 6-5T, was isolated from saline-alkaline soil from Songnen Plain in north-eastern China.
33104820	6	72	dep	had	570:572	arg1	%					749:749	97.4%	745:749	97.4%	745:749	The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences.
33104820	8	73	theme	halobia	1094:1100	arg1	1.2323T					1108:1114	N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T	1041:1114	N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T	1041:1114	The DNA‒DNA relatedness (DDH) values of isolate Hz 6-5T with N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T were 21.2%, 36.5%, and 32.0%, respectively.
33104820	6	74	theme	gene	591:594	arg1	similarities					605:616	highest 16S rRNA gene sequence similarities	574:616	highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T	574:742	The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences.
33104820	6	75	theme	rRNA	872:875	arg1	sequences					882:890	the 16S rRNA gene sequences	864:890	the 16S rRNA gene sequences	864:890	The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences.
33104820	6	76	contain	had	570:572	arg1	isolate					562:568	The isolate	558:568	The isolate	558:568	The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences.
33104820	6	76	contain	had	570:572	arg2	similarities					605:616	highest 16S rRNA gene sequence similarities	574:616	highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T	574:742	The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences.
33104820	6	77	theme	16S	582:584	arg1	similarities					605:616	highest 16S rRNA gene sequence similarities	574:616	highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T	574:742	The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences.
33104820	4	78	theme	0.5	478:480	arg1	%					481:481	%	481:481	%	481:481	The strain was aerobic with optimal growth at 33 °C, pH 9.0, and in the presence of 0.5% (w/v) NaCl or 3% (w/v) KCl.
33104820	13	79	theme	strain	1803:1808	arg1	6-5T					1813:1816	strain Hz 6-5T	1803:1816	strain Hz 6-5T	1803:1816	Based on the results of phylogenetic analysis supported by morphological, physiological, chemotaxonomic, and other differentiating phenotypic evidence, strain Hz 6-5T is considered to represent a novel species of the genus Nesterenkonia, for which the name Nesterenkonia haasae sp.
33104820	3	80	theme	Gram-staining	324:336	arg1	rods					388:391	Gram-staining positive, non-motile, and non-spore-forming short rods	324:391	Gram-staining positive, non-motile, and non-spore-forming short rods	324:391	The isolate formed light yellow-colored colonies and its cells were Gram-staining positive, non-motile, and non-spore-forming short rods.
33104820	6	81	theme	neighbor-joining	833:848	arg1	tree					850:853	the neighbor-joining tree	829:853	the neighbor-joining tree based on the 16S rRNA gene sequences	829:890	The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences.
33104820	3	82	theme	non-spore-forming	364:380	arg1	rods					388:391	Gram-staining positive, non-motile, and non-spore-forming short rods	324:391	Gram-staining positive, non-motile, and non-spore-forming short rods	324:391	The isolate formed light yellow-colored colonies and its cells were Gram-staining positive, non-motile, and non-spore-forming short rods.
33104820	12	83	theme	isolate	1624:1630	arg1	6-5T					1635:1638	isolate Hz 6-5T	1624:1638	isolate Hz 6-5T	1624:1638	The DNA G + C content of isolate Hz 6-5T was 60.8%.
33104820	8	84	with	values	1010:1015	arg1	1.2323T					1108:1114	N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T	1041:1114	N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T	1041:1114	The DNA‒DNA relatedness (DDH) values of isolate Hz 6-5T with N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T were 21.2%, 36.5%, and 32.0%, respectively.
33104820	0	85	theme	haasae	14:19	arg1	sp					21:22	Nesterenkonia haasae sp	0:22	Nesterenkonia haasae sp.	0:23	Nesterenkonia haasae sp.
33104820	12	86	theme	6-5T	1635:1638	arg1	%					1648:1648	60.8%	1644:1648	60.8%	1644:1648	The DNA G + C content of isolate Hz 6-5T was 60.8%.
33104820	12	86	theme	6-5T	1635:1638	arg1	content					1613:1619	The DNA G + C content	1599:1619	The DNA G + C content of isolate Hz 6-5T	1599:1638	The DNA G + C content of isolate Hz 6-5T was 60.8%.
33104820	1	87	theme	degraded	79:86	arg1	pasture					88:94	a degraded pasture	77:94	a degraded pasture in Songnen Plain	77:111	nov., an alkaliphilic actinobacterium isolated from a degraded pasture in Songnen Plain.
33104820	2	88	from	Plain	226:230	arg1	China					249:253	China	249:253	China	249:253	An alkaliphilic actinobacterial strain, designated Hz 6-5T, was isolated from saline-alkaline soil from Songnen Plain in north-eastern China.
33104820	2	89	attach	isolated	178:185	arg1	Plain					226:230	Songnen Plain	218:230	Songnen Plain in north-eastern China	218:253	An alkaliphilic actinobacterial strain, designated Hz 6-5T, was isolated from saline-alkaline soil from Songnen Plain in north-eastern China.
33104820	2	89	attach	isolated	178:185	arg2	strain					146:151	An alkaliphilic actinobacterial strain	114:151	An alkaliphilic actinobacterial strain	114:151	An alkaliphilic actinobacterial strain, designated Hz 6-5T, was isolated from saline-alkaline soil from Songnen Plain in north-eastern China.
33104820	2	89	attach	isolated	178:185	arg1	soil					208:211	saline-alkaline soil	192:211	saline-alkaline soil	192:211	An alkaliphilic actinobacterial strain, designated Hz 6-5T, was isolated from saline-alkaline soil from Songnen Plain in north-eastern China.
33104820	4	90	with	aerobic	409:415	arg1	growth					430:435	optimal growth	422:435	optimal growth at 33 °C, pH 9.0,	422:453	The strain was aerobic with optimal growth at 33 °C, pH 9.0, and in the presence of 0.5% (w/v) NaCl or 3% (w/v) KCl.
33104820	4	91	theme	%	498:498	arg1	KCl					506:508	3% (w/v) KCl	497:508	3% (w/v) KCl	497:508	The strain was aerobic with optimal growth at 33 °C, pH 9.0, and in the presence of 0.5% (w/v) NaCl or 3% (w/v) KCl.
33104820	15	92	theme	=CPCC	1979:1983	arg1	6-5T					1973:1976	Hz 6-5T	1970:1976	Hz 6-5T (=CPCC 205100T=NBRC 113521T)	1970:2005	The type strain is Hz 6-5T (=CPCC 205100T=NBRC 113521T).
33104820	15	92	theme	=CPCC	1979:1983	arg1	113521T					1998:2004	=CPCC 205100T=NBRC 113521T	1979:2004	=CPCC 205100T=NBRC 113521T	1979:2004	The type strain is Hz 6-5T (=CPCC 205100T=NBRC 113521T).
33104820	12	93	theme	DNA	1603:1605	arg1	%					1648:1648	60.8%	1644:1648	60.8%	1644:1648	The DNA G + C content of isolate Hz 6-5T was 60.8%.
33104820	12	93	theme	DNA	1603:1605	arg1	content					1613:1619	The DNA G + C content	1599:1619	The DNA G + C content of isolate Hz 6-5T	1599:1638	The DNA G + C content of isolate Hz 6-5T was 60.8%.
33104820	8	94	dep	N.	1062:1063	arg1	halophila					1065:1073	N. halophila DSM 16378T	1062:1084	N. halophila DSM 16378T	1062:1084	The DNA‒DNA relatedness (DDH) values of isolate Hz 6-5T with N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T were 21.2%, 36.5%, and 32.0%, respectively.
33104820	8	94	dep	N.	1062:1063	arg1	16378T					1079:1084	DSM 16378T	1075:1084	N. halophila DSM 16378T	1062:1084	The DNA‒DNA relatedness (DDH) values of isolate Hz 6-5T with N. natronophila M8T, N. halophila DSM 16378T, and N. halobia CGMCC 1.2323T were 21.2%, 36.5%, and 32.0%, respectively.
33104820	13	95	dep	morphological	1710:1722	arg1	phenotypic					1782:1791	phenotypic	1782:1791	phenotypic	1782:1791	Based on the results of phylogenetic analysis supported by morphological, physiological, chemotaxonomic, and other differentiating phenotypic evidence, strain Hz 6-5T is considered to represent a novel species of the genus Nesterenkonia, for which the name Nesterenkonia haasae sp.
33104820	13	95	dep	morphological	1710:1722	arg1	differentiating					1766:1780	differentiating	1766:1780	differentiating	1766:1780	Based on the results of phylogenetic analysis supported by morphological, physiological, chemotaxonomic, and other differentiating phenotypic evidence, strain Hz 6-5T is considered to represent a novel species of the genus Nesterenkonia, for which the name Nesterenkonia haasae sp.
33104820	4	96	dep	%	481:481	arg1	w/v					484:486	w/v	484:486	w/v	484:486	The strain was aerobic with optimal growth at 33 °C, pH 9.0, and in the presence of 0.5% (w/v) NaCl or 3% (w/v) KCl.
33104820	15	97	theme	type	1955:1958	arg1	strain					1960:1965	The type strain	1951:1965	The type strain	1951:1965	The type strain is Hz 6-5T (=CPCC 205100T=NBRC 113521T).
33104820	15	97	theme	type	1955:1958	arg1	6-5T					1973:1976	Hz 6-5T	1970:1976	Hz 6-5T (=CPCC 205100T=NBRC 113521T)	1970:2005	The type strain is Hz 6-5T (=CPCC 205100T=NBRC 113521T).
33104820	6	98	dep	strains	630:636	arg1	strains					630:636	the type strains	621:636	the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T	621:742	The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences.
33104820	6	98	dep	strains	630:636	arg1	GY074T					704:709	GY074T	704:709	GY074T	704:709	The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences.
33104820	6	98	dep	strains	630:636	arg1	%					688:688	98.2%	684:688	98.2%	684:688	The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences.
33104820	6	98	dep	strains	630:636	arg1	%					716:716	98.1%	712:716	98.1%	712:716	The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences.
33104820	6	98	dep	strains	630:636	arg1	GY239T					737:742	GY239T	737:742	GY239T	737:742	The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences.
33104820	6	98	dep	strains	630:636	arg1	M8T					679:681	M8T	679:681	M8T	679:681	The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences.
33104820	15	99	theme	205100T=NBRC	1985:1996	arg1	6-5T					1973:1976	Hz 6-5T	1970:1976	Hz 6-5T (=CPCC 205100T=NBRC 113521T)	1970:2005	The type strain is Hz 6-5T (=CPCC 205100T=NBRC 113521T).
33104820	15	99	theme	205100T=NBRC	1985:1996	arg1	113521T					1998:2004	=CPCC 205100T=NBRC 113521T	1979:2004	=CPCC 205100T=NBRC 113521T	1979:2004	The type strain is Hz 6-5T (=CPCC 205100T=NBRC 113521T).
33104820	6	100	with	subclade	778:785	arg1	strains					801:807	the type strains	792:807	the type strains	792:807	The isolate had highest 16S rRNA gene sequence similarities to the type strains of the species Nesternkonia natronophila M8T (98.2%), N. salmonea GY074T (98.1%), and N. sphaerica GY239T (97.4%), and the isolate formed a subclade with the type strains of these species in the neighbor-joining tree based on the 16S rRNA gene sequences.
33104820	11	101	theme	unidentified	1571:1582	arg1	phospholipids					1584:1596	two unidentified phospholipids	1567:1596	two unidentified phospholipids	1567:1596	The polar lipids detected for strain Hz 6-5T were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol, an unidentified glycolipid, and two unidentified phospholipids.
33104820	11	101	theme	unidentified	1571:1582	arg1	diphosphatidylglycerol					1446:1467	diphosphatidylglycerol	1446:1467	diphosphatidylglycerol	1446:1467	The polar lipids detected for strain Hz 6-5T were diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol, an unidentified glycolipid, and two unidentified phospholipids.
33104820	9	102	theme	characteristic	1164:1177	arg1	acid					1187:1190	The characteristic diamino acid	1160:1190	The characteristic diamino acid of strain Hz 6-5T	1160:1208	The characteristic diamino acid of strain Hz 6-5T was found to be lysine.
33104820	9	102	theme	characteristic	1164:1177	arg1	lysine					1226:1231	lysine	1226:1231	lysine	1226:1231	The characteristic diamino acid of strain Hz 6-5T was found to be lysine.
33104820	2	103	theme	alkaliphilic	117:128	arg1	strain					146:151	An alkaliphilic actinobacterial strain	114:151	An alkaliphilic actinobacterial strain	114:151	An alkaliphilic actinobacterial strain, designated Hz 6-5T, was isolated from saline-alkaline soil from Songnen Plain in north-eastern China.
33104820	13	104	theme	name	1903:1906	arg1	sp					1929:1930	the name Nesterenkonia haasae sp	1899:1930	the name Nesterenkonia haasae sp	1899:1930	Based on the results of phylogenetic analysis supported by morphological, physiological, chemotaxonomic, and other differentiating phenotypic evidence, strain Hz 6-5T is considered to represent a novel species of the genus Nesterenkonia, for which the name Nesterenkonia haasae sp.
33432773	13	0	dep	hemolysis	1621:1629	arg1	%					1619:1619	%	1619:1619	%	1619:1619	Furthermore, the biocomposites exhibited better antibacterial activity, no effect on the blood clotting (normal in vitro blood clotting), and less than 5% hemolysis.
33432773	4	1	theme	structural	512:521	arg1	changes					523:529	structural changes	512:529	structural changes	512:529	The samples were characterized for structural changes, surface morphology, porosity, and mechanical properties through spectroscopic (Fourier transform-infrared [FT-IR]), microscopic (scanning electron microscope [SEM]), X-ray (powder X-ray diffraction and energy-dispersive X-ray), and other analytical (Brunauer-Emmett-Teller, universal testing machine Instron) techniques.
33432773	4	2	theme	powder	705:710	arg1	diffraction					718:728	powder X-ray diffraction	705:728	powder X-ray diffraction	705:728	The samples were characterized for structural changes, surface morphology, porosity, and mechanical properties through spectroscopic (Fourier transform-infrared [FT-IR]), microscopic (scanning electron microscope [SEM]), X-ray (powder X-ray diffraction and energy-dispersive X-ray), and other analytical (Brunauer-Emmett-Teller, universal testing machine Instron) techniques.
33432773	1	3	theme	tissue	266:271	arg1	regeneration					273:284	the implantation and tissue regeneration	245:284	regeneration	273:284	The importance of bone scaffolds has increased many folds in the last few years; however, during bone implantation, bacterial infections compromise the implantation and tissue regeneration.
33432773	8	4	theme	-67.28	1115:1120	arg1	%					1114:1114	%	1114:1114	%	1114:1114	The percentage porosity was in the range of 49.75%-67.28%, and the pore size was 215.65-470.87 µm.
33432773	4	5	theme	surface	532:538	arg1	morphology					540:549	surface morphology	532:549	surface morphology	532:549	The samples were characterized for structural changes, surface morphology, porosity, and mechanical properties through spectroscopic (Fourier transform-infrared [FT-IR]), microscopic (scanning electron microscope [SEM]), X-ray (powder X-ray diffraction and energy-dispersive X-ray), and other analytical (Brunauer-Emmett-Teller, universal testing machine Instron) techniques.
33432773	11	6	theme	nanohydroxyapatite	1323:1340	arg1	presence					1311:1318	the presence	1307:1318	the presence of nanohydroxyapatite and graphene oxide (GO)	1307:1364	X-ray studies confirmed the presence of nanohydroxyapatite and graphene oxide (GO).
33432773	9	7	theme	cellular	1194:1201	arg1	penetration					1203:1213	cellular penetration	1194:1213	cellular penetration	1194:1213	The pore size was perfect for cellular penetration.
33432773	13	8	dep	in	1578:1579	arg1	vitro					1581:1585	vitro	1581:1585	vitro	1581:1585	Furthermore, the biocomposites exhibited better antibacterial activity, no effect on the blood clotting (normal in vitro blood clotting), and less than 5% hemolysis.
33432773	12	9	theme	biocomposite	1452:1463	arg1	toxicity					1436:1443	no significant toxicity	1421:1443	no significant toxicity of the biocomposite	1421:1463	The cell viability was 85%-98% (BS1-BS3), which shows no significant toxicity of the biocomposite.
33432773	4	10	theme	transform-infrared	619:636	arg1	spectroscopic					596:608	spectroscopic	596:608	spectroscopic	596:608	The samples were characterized for structural changes, surface morphology, porosity, and mechanical properties through spectroscopic (Fourier transform-infrared [FT-IR]), microscopic (scanning electron microscope [SEM]), X-ray (powder X-ray diffraction and energy-dispersive X-ray), and other analytical (Brunauer-Emmett-Teller, universal testing machine Instron) techniques.
33432773	4	10	theme	transform-infrared	619:636	arg1	[FT-IR					638:643	Fourier transform-infrared [FT-IR	611:643	Fourier transform-infrared [FT-IR	611:643	The samples were characterized for structural changes, surface morphology, porosity, and mechanical properties through spectroscopic (Fourier transform-infrared [FT-IR]), microscopic (scanning electron microscope [SEM]), X-ray (powder X-ray diffraction and energy-dispersive X-ray), and other analytical (Brunauer-Emmett-Teller, universal testing machine Instron) techniques.
33432773	8	11	theme	49.75	1109:1113	arg1	%					1114:1114	%	1114:1114	%	1114:1114	The percentage porosity was in the range of 49.75%-67.28%, and the pore size was 215.65-470.87 µm.
33432773	5	12	theme	standard	928:935	arg1	protocols					937:945	standard protocols	928:945	standard protocols	928:945	Antibacterial, cellular, and hemocompatibility assays were performed using standard protocols.
33432773	13	13	from	effect	1541:1546	arg1	clotting					1561:1568	the blood clotting	1551:1568	the blood clotting (normal in vitro blood clotting)	1551:1601	Furthermore, the biocomposites exhibited better antibacterial activity, no effect on the blood clotting (normal in vitro blood clotting), and less than 5% hemolysis.
33432773	13	13	from	effect	1541:1546	arg1	clotting					1593:1600	normal in vitro blood clotting	1571:1600	normal in vitro blood clotting	1571:1600	Furthermore, the biocomposites exhibited better antibacterial activity, no effect on the blood clotting (normal in vitro blood clotting), and less than 5% hemolysis.
33432773	4	14	theme	Fourier	611:617	arg1	spectroscopic					596:608	spectroscopic	596:608	spectroscopic	596:608	The samples were characterized for structural changes, surface morphology, porosity, and mechanical properties through spectroscopic (Fourier transform-infrared [FT-IR]), microscopic (scanning electron microscope [SEM]), X-ray (powder X-ray diffraction and energy-dispersive X-ray), and other analytical (Brunauer-Emmett-Teller, universal testing machine Instron) techniques.
33432773	4	14	theme	Fourier	611:617	arg1	[FT-IR					638:643	Fourier transform-infrared [FT-IR	611:643	Fourier transform-infrared [FT-IR	611:643	The samples were characterized for structural changes, surface morphology, porosity, and mechanical properties through spectroscopic (Fourier transform-infrared [FT-IR]), microscopic (scanning electron microscope [SEM]), X-ray (powder X-ray diffraction and energy-dispersive X-ray), and other analytical (Brunauer-Emmett-Teller, universal testing machine Instron) techniques.
33432773	11	15	theme	oxide	1355:1359	arg1	presence					1311:1318	the presence	1307:1318	the presence of nanohydroxyapatite and graphene oxide (GO)	1307:1364	X-ray studies confirmed the presence of nanohydroxyapatite and graphene oxide (GO).
33432773	1	16	dep	many	144:147	arg1	folds					149:153	folds	149:153	folds	149:153	The importance of bone scaffolds has increased many folds in the last few years; however, during bone implantation, bacterial infections compromise the implantation and tissue regeneration.
33432773	13	17	theme	antibacterial	1514:1526	arg1	activity					1528:1535	better antibacterial activity	1507:1535	better antibacterial activity	1507:1535	Furthermore, the biocomposites exhibited better antibacterial activity, no effect on the blood clotting (normal in vitro blood clotting), and less than 5% hemolysis.
33432773	15	18	theme	tissue	1854:1859	arg1	engineering					1861:1871	bone tissue engineering	1849:1871	bone tissue engineering	1849:1871	These exciting results revealed that this material has the potential for possible application in bone tissue engineering.
33432773	10	19	theme	significant	1235:1245	arg1	proliferation					1247:1259	significant proliferation	1235:1259	significant proliferation	1235:1259	Thus, cells showed significant proliferation onto these scaffolds.
33432773	0	20	theme	Arabinoxylan/graphene-oxide/nHAp-NPs/PVA	0:39	arg1	composite					49:57	Arabinoxylan/graphene-oxide/nHAp-NPs/PVA bionano composite	0:57	Arabinoxylan/graphene-oxide/nHAp-NPs/PVA bionano composite	0:57	Arabinoxylan/graphene-oxide/nHAp-NPs/PVA bionano composite scaffolds for fractured bone healing.
33432773	13	21	theme	better	1507:1512	arg1	activity					1528:1535	better antibacterial activity	1507:1535	better antibacterial activity	1507:1535	Furthermore, the biocomposites exhibited better antibacterial activity, no effect on the blood clotting (normal in vitro blood clotting), and less than 5% hemolysis.
33432773	0	22	theme	bionano	41:47	arg1	composite					49:57	Arabinoxylan/graphene-oxide/nHAp-NPs/PVA bionano composite	0:57	Arabinoxylan/graphene-oxide/nHAp-NPs/PVA bionano composite	0:57	Arabinoxylan/graphene-oxide/nHAp-NPs/PVA bionano composite scaffolds for fractured bone healing.
33432773	12	23	theme	significant	1424:1434	arg1	toxicity					1436:1443	no significant toxicity	1421:1443	no significant toxicity of the biocomposite	1421:1463	The cell viability was 85%-98% (BS1-BS3), which shows no significant toxicity of the biocomposite.
33432773	11	24	theme	graphene	1346:1353	arg1	oxide					1355:1359	graphene oxide	1346:1359	graphene oxide (GO)	1346:1364	X-ray studies confirmed the presence of nanohydroxyapatite and graphene oxide (GO).
33432773	1	25	theme	bone	115:118	arg1	scaffolds					120:128	bone scaffolds	115:128	bone scaffolds	115:128	The importance of bone scaffolds has increased many folds in the last few years; however, during bone implantation, bacterial infections compromise the implantation and tissue regeneration.
33432773	4	26	theme	electron	670:677	arg1	microscopic					648:658	microscopic	648:658	microscopic	648:658	The samples were characterized for structural changes, surface morphology, porosity, and mechanical properties through spectroscopic (Fourier transform-infrared [FT-IR]), microscopic (scanning electron microscope [SEM]), X-ray (powder X-ray diffraction and energy-dispersive X-ray), and other analytical (Brunauer-Emmett-Teller, universal testing machine Instron) techniques.
33432773	4	26	theme	electron	670:677	arg1	microscope					679:688	scanning electron microscope [SEM]	661:694	scanning electron microscope [SEM]	661:694	The samples were characterized for structural changes, surface morphology, porosity, and mechanical properties through spectroscopic (Fourier transform-infrared [FT-IR]), microscopic (scanning electron microscope [SEM]), X-ray (powder X-ray diffraction and energy-dispersive X-ray), and other analytical (Brunauer-Emmett-Teller, universal testing machine Instron) techniques.
33432773	3	27	theme	Biocomposite	400:411	arg1	BS					424:425	BS	424:425	BS	424:425	Biocomposite scaffolds (BS) were fabricated via the freeze-drying technique.
33432773	3	27	theme	Biocomposite	400:411	arg1	scaffolds					413:421	Biocomposite scaffolds	400:421	Biocomposite scaffolds (BS)	400:426	Biocomposite scaffolds (BS) were fabricated via the freeze-drying technique.
33432773	4	28	theme	mechanical	566:575	arg1	properties					577:586	mechanical properties	566:586	mechanical properties	566:586	The samples were characterized for structural changes, surface morphology, porosity, and mechanical properties through spectroscopic (Fourier transform-infrared [FT-IR]), microscopic (scanning electron microscope [SEM]), X-ray (powder X-ray diffraction and energy-dispersive X-ray), and other analytical (Brunauer-Emmett-Teller, universal testing machine Instron) techniques.
33432773	4	29	dep	X-ray	698:702	arg1	diffraction					718:728	powder X-ray diffraction	705:728	powder X-ray diffraction	705:728	The samples were characterized for structural changes, surface morphology, porosity, and mechanical properties through spectroscopic (Fourier transform-infrared [FT-IR]), microscopic (scanning electron microscope [SEM]), X-ray (powder X-ray diffraction and energy-dispersive X-ray), and other analytical (Brunauer-Emmett-Teller, universal testing machine Instron) techniques.
33432773	4	29	dep	X-ray	698:702	arg1	X-ray					752:756	energy-dispersive X-ray	734:756	energy-dispersive X-ray	734:756	The samples were characterized for structural changes, surface morphology, porosity, and mechanical properties through spectroscopic (Fourier transform-infrared [FT-IR]), microscopic (scanning electron microscope [SEM]), X-ray (powder X-ray diffraction and energy-dispersive X-ray), and other analytical (Brunauer-Emmett-Teller, universal testing machine Instron) techniques.
33432773	8	30	theme	percentage	1069:1078	arg1	porosity					1080:1087	The percentage porosity	1065:1087	The percentage porosity	1065:1087	The percentage porosity was in the range of 49.75%-67.28%, and the pore size was 215.65-470.87 µm.
33432773	1	31	theme	scaffolds	120:128	arg1	importance					101:110	The importance	97:110	The importance of bone scaffolds	97:128	The importance of bone scaffolds has increased many folds in the last few years; however, during bone implantation, bacterial infections compromise the implantation and tissue regeneration.
33432773	2	32	theme	bone	381:384	arg1	regeneration					386:397	bone regeneration	381:397	bone regeneration	381:397	This work is focused on this issue while not compromising on the properties of a scaffold for bone regeneration.
33432773	15	33	theme	possible	1825:1832	arg1	application					1834:1844	possible application	1825:1844	possible application in bone tissue engineering	1825:1871	These exciting results revealed that this material has the potential for possible application in bone tissue engineering.
33432773	5	34	dep	Antibacterial	853:865	arg1	assays					900:905	assays	900:905	assays	900:905	Antibacterial, cellular, and hemocompatibility assays were performed using standard protocols.
33432773	8	35	theme	%	1121:1121	arg1	range					1100:1104	the range	1096:1104	the range of 49.75%-67.28%	1096:1121	The percentage porosity was in the range of 49.75%-67.28%, and the pore size was 215.65-470.87 µm.
33432773	15	36	theme	exciting	1758:1765	arg1	results					1767:1773	These exciting results	1752:1773	These exciting results	1752:1773	These exciting results revealed that this material has the potential for possible application in bone tissue engineering.
33432773	12	37	theme	%	1392:1392	arg1	%					1396:1396	85%-98%	1390:1396	85%-98% (BS1-BS3)	1390:1406	The cell viability was 85%-98% (BS1-BS3), which shows no significant toxicity of the biocomposite.
33432773	12	37	theme	%	1392:1392	arg1	viability					1376:1384	The cell viability	1367:1384	The cell viability	1367:1384	The cell viability was 85%-98% (BS1-BS3), which shows no significant toxicity of the biocomposite.
33432773	12	37	theme	%	1392:1392	arg1	BS1-BS3					1399:1405	BS1-BS3	1399:1405	BS1-BS3	1399:1405	The cell viability was 85%-98% (BS1-BS3), which shows no significant toxicity of the biocomposite.
33432773	4	38	theme	Brunauer-Emmett-Teller	782:803	arg1	analytical					770:779	analytical	770:779	analytical	770:779	The samples were characterized for structural changes, surface morphology, porosity, and mechanical properties through spectroscopic (Fourier transform-infrared [FT-IR]), microscopic (scanning electron microscope [SEM]), X-ray (powder X-ray diffraction and energy-dispersive X-ray), and other analytical (Brunauer-Emmett-Teller, universal testing machine Instron) techniques.
33432773	4	38	theme	Brunauer-Emmett-Teller	782:803	arg1	Instron					832:838	Brunauer-Emmett-Teller, universal testing machine Instron	782:838	Instron	832:838	The samples were characterized for structural changes, surface morphology, porosity, and mechanical properties through spectroscopic (Fourier transform-infrared [FT-IR]), microscopic (scanning electron microscope [SEM]), X-ray (powder X-ray diffraction and energy-dispersive X-ray), and other analytical (Brunauer-Emmett-Teller, universal testing machine Instron) techniques.
33432773	13	39	theme	blood	1587:1591	arg1	clotting					1561:1568	the blood clotting	1551:1568	the blood clotting (normal in vitro blood clotting)	1551:1601	Furthermore, the biocomposites exhibited better antibacterial activity, no effect on the blood clotting (normal in vitro blood clotting), and less than 5% hemolysis.
33432773	13	39	theme	blood	1587:1591	arg1	clotting					1593:1600	normal in vitro blood clotting	1571:1600	normal in vitro blood clotting	1571:1600	Furthermore, the biocomposites exhibited better antibacterial activity, no effect on the blood clotting (normal in vitro blood clotting), and less than 5% hemolysis.
33432773	0	40	theme	fractured	73:81	arg1	healing					88:94	fractured bone healing	73:94	fractured bone healing	73:94	Arabinoxylan/graphene-oxide/nHAp-NPs/PVA bionano composite scaffolds for fractured bone healing.
33432773	6	41	theme	components	992:1001	arg1	interactions					968:979	the interactions	964:979	the interactions of all the components	964:1001	FT-IR confirmed the interactions of all the components.
33432773	1	42	theme	bacterial	213:221	arg1	infections					223:232	bacterial infections	213:232	bacterial infections	213:232	The importance of bone scaffolds has increased many folds in the last few years; however, during bone implantation, bacterial infections compromise the implantation and tissue regeneration.
33432773	13	43	theme	blood	1555:1559	arg1	clotting					1561:1568	the blood clotting	1551:1568	the blood clotting (normal in vitro blood clotting)	1551:1601	Furthermore, the biocomposites exhibited better antibacterial activity, no effect on the blood clotting (normal in vitro blood clotting), and less than 5% hemolysis.
33432773	13	43	theme	blood	1555:1559	arg1	clotting					1593:1600	normal in vitro blood clotting	1571:1600	normal in vitro blood clotting	1571:1600	Furthermore, the biocomposites exhibited better antibacterial activity, no effect on the blood clotting (normal in vitro blood clotting), and less than 5% hemolysis.
33432773	1	44	theme	bone	194:197	arg1	implantation					199:210	bone implantation	194:210	bone implantation	194:210	The importance of bone scaffolds has increased many folds in the last few years; however, during bone implantation, bacterial infections compromise the implantation and tissue regeneration.
33432773	3	45	theme	freeze-drying	452:464	arg1	technique					466:474	the freeze-drying technique	448:474	the freeze-drying technique	448:474	Biocomposite scaffolds (BS) were fabricated via the freeze-drying technique.
33432773	13	46	theme	in	1578:1579	arg1	clotting					1561:1568	the blood clotting	1551:1568	the blood clotting (normal in vitro blood clotting)	1551:1601	Furthermore, the biocomposites exhibited better antibacterial activity, no effect on the blood clotting (normal in vitro blood clotting), and less than 5% hemolysis.
33432773	13	46	theme	in	1578:1579	arg1	clotting					1593:1600	normal in vitro blood clotting	1571:1600	normal in vitro blood clotting	1571:1600	Furthermore, the biocomposites exhibited better antibacterial activity, no effect on the blood clotting (normal in vitro blood clotting), and less than 5% hemolysis.
33432773	13	47	from	activity	1528:1535	arg1	clotting					1561:1568	the blood clotting	1551:1568	the blood clotting (normal in vitro blood clotting)	1551:1601	Furthermore, the biocomposites exhibited better antibacterial activity, no effect on the blood clotting (normal in vitro blood clotting), and less than 5% hemolysis.
33432773	13	47	from	activity	1528:1535	arg1	clotting					1593:1600	normal in vitro blood clotting	1571:1600	normal in vitro blood clotting	1571:1600	Furthermore, the biocomposites exhibited better antibacterial activity, no effect on the blood clotting (normal in vitro blood clotting), and less than 5% hemolysis.
33432773	12	48	theme	cell	1371:1374	arg1	%					1396:1396	85%-98%	1390:1396	85%-98% (BS1-BS3)	1390:1406	The cell viability was 85%-98% (BS1-BS3), which shows no significant toxicity of the biocomposite.
33432773	12	48	theme	cell	1371:1374	arg1	viability					1376:1384	The cell viability	1367:1384	The cell viability	1367:1384	The cell viability was 85%-98% (BS1-BS3), which shows no significant toxicity of the biocomposite.
33432773	15	49	contain	has	1803:1805	arg1	material					1794:1801	this material	1789:1801	this material	1789:1801	These exciting results revealed that this material has the potential for possible application in bone tissue engineering.
33432773	15	49	contain	has	1803:1805	arg2	potential					1811:1819	the potential	1807:1819	the potential for possible application in bone tissue engineering	1807:1871	These exciting results revealed that this material has the potential for possible application in bone tissue engineering.
33432773	4	50	theme	scanning	661:668	arg1	microscopic					648:658	microscopic	648:658	microscopic	648:658	The samples were characterized for structural changes, surface morphology, porosity, and mechanical properties through spectroscopic (Fourier transform-infrared [FT-IR]), microscopic (scanning electron microscope [SEM]), X-ray (powder X-ray diffraction and energy-dispersive X-ray), and other analytical (Brunauer-Emmett-Teller, universal testing machine Instron) techniques.
33432773	4	50	theme	scanning	661:668	arg1	microscope					679:688	scanning electron microscope [SEM]	661:694	scanning electron microscope [SEM]	661:694	The samples were characterized for structural changes, surface morphology, porosity, and mechanical properties through spectroscopic (Fourier transform-infrared [FT-IR]), microscopic (scanning electron microscope [SEM]), X-ray (powder X-ray diffraction and energy-dispersive X-ray), and other analytical (Brunauer-Emmett-Teller, universal testing machine Instron) techniques.
33432773	11	51	dep	oxide	1355:1359	arg1	GO					1362:1363	GO	1362:1363	GO	1362:1363	X-ray studies confirmed the presence of nanohydroxyapatite and graphene oxide (GO).
33432773	8	52	theme	pore	1132:1135	arg1	215.65-470.87 µm					1146:1161	215.65-470.87 µm	1146:1161	215.65-470.87 µm	1146:1161	The percentage porosity was in the range of 49.75%-67.28%, and the pore size was 215.65-470.87 µm.
33432773	8	52	theme	pore	1132:1135	arg1	size					1137:1140	the pore size	1128:1140	the pore size	1128:1140	The percentage porosity was in the range of 49.75%-67.28%, and the pore size was 215.65-470.87 µm.
33432773	4	53	dep	spectroscopic	596:608	arg1	techniques					841:850	techniques	841:850	techniques	841:850	The samples were characterized for structural changes, surface morphology, porosity, and mechanical properties through spectroscopic (Fourier transform-infrared [FT-IR]), microscopic (scanning electron microscope [SEM]), X-ray (powder X-ray diffraction and energy-dispersive X-ray), and other analytical (Brunauer-Emmett-Teller, universal testing machine Instron) techniques.
33432773	15	54	theme	bone	1849:1852	arg1	engineering					1861:1871	bone tissue engineering	1849:1871	bone tissue engineering	1849:1871	These exciting results revealed that this material has the potential for possible application in bone tissue engineering.
33432773	0	55	theme	bone	83:86	arg1	healing					88:94	fractured bone healing	73:94	fractured bone healing	73:94	Arabinoxylan/graphene-oxide/nHAp-NPs/PVA bionano composite scaffolds for fractured bone healing.
33432773	14	56	dep	content	1743:1749	arg1	GO					1740:1741	GO	1740:1741	GO	1740:1741	The ultimate compression strength for the biocomposites increased from 4.05 to 7.94 with an increase in the GO content.
33432773	4	57	theme	X-ray	712:716	arg1	diffraction					718:728	powder X-ray diffraction	705:728	powder X-ray diffraction	705:728	The samples were characterized for structural changes, surface morphology, porosity, and mechanical properties through spectroscopic (Fourier transform-infrared [FT-IR]), microscopic (scanning electron microscope [SEM]), X-ray (powder X-ray diffraction and energy-dispersive X-ray), and other analytical (Brunauer-Emmett-Teller, universal testing machine Instron) techniques.
33432773	13	58	theme	normal	1571:1576	arg1	clotting					1561:1568	the blood clotting	1551:1568	the blood clotting (normal in vitro blood clotting)	1551:1601	Furthermore, the biocomposites exhibited better antibacterial activity, no effect on the blood clotting (normal in vitro blood clotting), and less than 5% hemolysis.
33432773	13	58	theme	normal	1571:1576	arg1	clotting					1593:1600	normal in vitro blood clotting	1571:1600	normal in vitro blood clotting	1571:1600	Furthermore, the biocomposites exhibited better antibacterial activity, no effect on the blood clotting (normal in vitro blood clotting), and less than 5% hemolysis.
33432773	4	59	theme	universal	806:814	arg1	analytical					770:779	analytical	770:779	analytical	770:779	The samples were characterized for structural changes, surface morphology, porosity, and mechanical properties through spectroscopic (Fourier transform-infrared [FT-IR]), microscopic (scanning electron microscope [SEM]), X-ray (powder X-ray diffraction and energy-dispersive X-ray), and other analytical (Brunauer-Emmett-Teller, universal testing machine Instron) techniques.
33432773	4	59	theme	universal	806:814	arg1	Instron					832:838	Brunauer-Emmett-Teller, universal testing machine Instron	782:838	Instron	832:838	The samples were characterized for structural changes, surface morphology, porosity, and mechanical properties through spectroscopic (Fourier transform-infrared [FT-IR]), microscopic (scanning electron microscope [SEM]), X-ray (powder X-ray diffraction and energy-dispersive X-ray), and other analytical (Brunauer-Emmett-Teller, universal testing machine Instron) techniques.
33432773	9	60	theme	pore	1168:1171	arg1	perfect					1182:1188	perfect	1182:1188	perfect	1182:1188	The pore size was perfect for cellular penetration.
33432773	9	60	theme	pore	1168:1171	arg1	size					1173:1176	The pore size	1164:1176	The pore size	1164:1176	The pore size was perfect for cellular penetration.
33432773	4	61	theme	energy-dispersive	734:750	arg1	X-ray					752:756	energy-dispersive X-ray	734:756	energy-dispersive X-ray	734:756	The samples were characterized for structural changes, surface morphology, porosity, and mechanical properties through spectroscopic (Fourier transform-infrared [FT-IR]), microscopic (scanning electron microscope [SEM]), X-ray (powder X-ray diffraction and energy-dispersive X-ray), and other analytical (Brunauer-Emmett-Teller, universal testing machine Instron) techniques.
33432773	14	62	from	increase	1724:1731	arg1	content					1743:1749	the GO content	1736:1749	the GO content	1736:1749	The ultimate compression strength for the biocomposites increased from 4.05 to 7.94 with an increase in the GO content.
33432773	14	63	dep	7.94	1711:1714	arg1	to					1708:1709	to	1708:1709	to	1708:1709	The ultimate compression strength for the biocomposites increased from 4.05 to 7.94 with an increase in the GO content.
33432773	11	64	theme	X-ray	1283:1287	arg1	studies					1289:1295	X-ray studies	1283:1295	X-ray studies	1283:1295	X-ray studies confirmed the presence of nanohydroxyapatite and graphene oxide (GO).
33432773	15	65	from	application	1834:1844	arg1	engineering					1861:1871	bone tissue engineering	1849:1871	bone tissue engineering	1849:1871	These exciting results revealed that this material has the potential for possible application in bone tissue engineering.
33432773	7	66	theme	interconnected	1031:1044	arg1	morphology					1053:1062	porous and interconnected porous morphology	1020:1062	porous and interconnected porous morphology	1020:1062	SEM illustrated porous and interconnected porous morphology.
33432773	1	67	theme	few	167:169	arg1	years					171:175	the last few years	158:175	the last few years	158:175	The importance of bone scaffolds has increased many folds in the last few years; however, during bone implantation, bacterial infections compromise the implantation and tissue regeneration.
33432773	14	68	theme	compression	1645:1655	arg1	strength					1657:1664	The ultimate compression strength	1632:1664	The ultimate compression strength for the biocomposites	1632:1686	The ultimate compression strength for the biocomposites increased from 4.05 to 7.94 with an increase in the GO content.
33432773	4	69	theme	machine	824:830	arg1	analytical					770:779	analytical	770:779	analytical	770:779	The samples were characterized for structural changes, surface morphology, porosity, and mechanical properties through spectroscopic (Fourier transform-infrared [FT-IR]), microscopic (scanning electron microscope [SEM]), X-ray (powder X-ray diffraction and energy-dispersive X-ray), and other analytical (Brunauer-Emmett-Teller, universal testing machine Instron) techniques.
33432773	4	69	theme	machine	824:830	arg1	Instron					832:838	Brunauer-Emmett-Teller, universal testing machine Instron	782:838	Instron	832:838	The samples were characterized for structural changes, surface morphology, porosity, and mechanical properties through spectroscopic (Fourier transform-infrared [FT-IR]), microscopic (scanning electron microscope [SEM]), X-ray (powder X-ray diffraction and energy-dispersive X-ray), and other analytical (Brunauer-Emmett-Teller, universal testing machine Instron) techniques.
33432773	7	70	theme	porous	1046:1051	arg1	morphology					1053:1062	porous and interconnected porous morphology	1020:1062	porous and interconnected porous morphology	1020:1062	SEM illustrated porous and interconnected porous morphology.
33432773	1	71	theme	last	162:165	arg1	years					171:175	the last few years	158:175	the last few years	158:175	The importance of bone scaffolds has increased many folds in the last few years; however, during bone implantation, bacterial infections compromise the implantation and tissue regeneration.
33432773	1	72	theme	implantation	249:260	arg1	regeneration					273:284	the implantation and tissue regeneration	245:284	regeneration	273:284	The importance of bone scaffolds has increased many folds in the last few years; however, during bone implantation, bacterial infections compromise the implantation and tissue regeneration.
33432773	13	73	from	hemolysis	1621:1629	arg1	clotting					1561:1568	the blood clotting	1551:1568	the blood clotting (normal in vitro blood clotting)	1551:1601	Furthermore, the biocomposites exhibited better antibacterial activity, no effect on the blood clotting (normal in vitro blood clotting), and less than 5% hemolysis.
33432773	13	73	from	hemolysis	1621:1629	arg1	clotting					1593:1600	normal in vitro blood clotting	1571:1600	normal in vitro blood clotting	1571:1600	Furthermore, the biocomposites exhibited better antibacterial activity, no effect on the blood clotting (normal in vitro blood clotting), and less than 5% hemolysis.
33432773	14	74	theme	ultimate	1636:1643	arg1	strength					1657:1664	The ultimate compression strength	1632:1664	The ultimate compression strength for the biocomposites	1632:1686	The ultimate compression strength for the biocomposites increased from 4.05 to 7.94 with an increase in the GO content.
33432773	4	75	theme	testing	816:822	arg1	analytical					770:779	analytical	770:779	analytical	770:779	The samples were characterized for structural changes, surface morphology, porosity, and mechanical properties through spectroscopic (Fourier transform-infrared [FT-IR]), microscopic (scanning electron microscope [SEM]), X-ray (powder X-ray diffraction and energy-dispersive X-ray), and other analytical (Brunauer-Emmett-Teller, universal testing machine Instron) techniques.
33432773	4	75	theme	testing	816:822	arg1	Instron					832:838	Brunauer-Emmett-Teller, universal testing machine Instron	782:838	Instron	832:838	The samples were characterized for structural changes, surface morphology, porosity, and mechanical properties through spectroscopic (Fourier transform-infrared [FT-IR]), microscopic (scanning electron microscope [SEM]), X-ray (powder X-ray diffraction and energy-dispersive X-ray), and other analytical (Brunauer-Emmett-Teller, universal testing machine Instron) techniques.
33432773	7	76	theme	porous	1020:1025	arg1	morphology					1053:1062	porous and interconnected porous morphology	1020:1062	porous and interconnected porous morphology	1020:1062	SEM illustrated porous and interconnected porous morphology.
33432773	2	77	theme	scaffold	368:375	arg1	properties					352:361	the properties	348:361	the properties of a scaffold for bone regeneration	348:397	This work is focused on this issue while not compromising on the properties of a scaffold for bone regeneration.
33432773	13	78	dep	%	1619:1619	arg1	5					1618:1618	5	1618:1618	5	1618:1618	Furthermore, the biocomposites exhibited better antibacterial activity, no effect on the blood clotting (normal in vitro blood clotting), and less than 5% hemolysis.
34634561	8	0	theme	Akkermansia	1655:1665	arg1	genera					1667:1672	the Parabacteroides, Sutterella, Allobaculum, and Akkermansia genera	1605:1672	the Parabacteroides, Sutterella, Allobaculum, and Akkermansia genera	1605:1672	The Lachnospiraceae and Enterobacteriaceae families and the Parabacteroides, Sutterella, Allobaculum, and Akkermansia genera were enriched more in the HF-J10 and HF-J15 groups than in the HF group.
34634561	2	1	theme	jaboticaba	347:356	arg1	peel					358:361	polyphenol-rich jaboticaba peel	331:361	polyphenol-rich jaboticaba peel	331:361	We evaluated the effect of polyphenol-rich jaboticaba peel and seed powder (JPSP) on the gut microbial community composition and liver health in a mouse model of NAFLD.
34634561	7	2	theme	drastic	1234:1240	arg1	depletion					1242:1250	a drastic depletion	1232:1250	a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae)	1232:1452	The effects on the microbial community structure were determined in all JPSP-supplemented groups; however, the HF-J10 and HF-J15 diets led to a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae) compared to the HF diet, some of which represented a reversal of increases associated with HF.
34634561	7	3	theme	HF-J10	1201:1206	arg1	diets					1219:1223	the HF-J10 and HF-J15 diets	1197:1223	diets	1219:1223	The effects on the microbial community structure were determined in all JPSP-supplemented groups; however, the HF-J10 and HF-J15 diets led to a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae) compared to the HF diet, some of which represented a reversal of increases associated with HF.
34634561	3	4	theme	Three-month-old	473:487	arg1	mice					504:507	Three-month-old C57BL/6 J male mice	473:507	Three-month-old C57BL/6 J male mice	473:507	Three-month-old C57BL/6 J male mice, received either a control (C, 10% of lipids as energy, n = 16) or high-fat (HF, 50% of lipids as energy, n = 64) diet for nine weeks.
34634561	1	5	theme	fatty	275:279	arg1	NAFLD					296:300	NAFLD	296:300	NAFLD	296:300	The consumption of a high-fat diet can cause metabolic syndrome and induces host gut microbial dysbiosis and non-alcoholic fatty liver disease (NAFLD).
34634561	1	5	theme	fatty	275:279	arg1	disease					287:293	non-alcoholic fatty liver disease	261:293	non-alcoholic fatty liver disease (NAFLD)	261:301	The consumption of a high-fat diet can cause metabolic syndrome and induces host gut microbial dysbiosis and non-alcoholic fatty liver disease (NAFLD).
34634561	1	6	theme	high-fat	173:180	arg1	diet					182:185	a high-fat diet	171:185	a high-fat diet	171:185	The consumption of a high-fat diet can cause metabolic syndrome and induces host gut microbial dysbiosis and non-alcoholic fatty liver disease (NAFLD).
34634561	6	7	theme	lipogenesis	1036:1046	arg1	ABCG8					1082:1086	ABCG8	1082:1086	ABCG8	1082:1086	In a dose-dependent manner, JPSP consumption ameliorated the expression of hepatic lipogenesis genes (AMPK, SREBP-1, HGMCoA, and ABCG8).
34634561	6	7	theme	lipogenesis	1036:1046	arg1	SREBP-1					1061:1067	SREBP-1	1061:1067	SREBP-1	1061:1067	In a dose-dependent manner, JPSP consumption ameliorated the expression of hepatic lipogenesis genes (AMPK, SREBP-1, HGMCoA, and ABCG8).
34634561	6	7	theme	lipogenesis	1036:1046	arg1	AMPK					1055:1058	AMPK	1055:1058	AMPK	1055:1058	In a dose-dependent manner, JPSP consumption ameliorated the expression of hepatic lipogenesis genes (AMPK, SREBP-1, HGMCoA, and ABCG8).
34634561	6	7	theme	lipogenesis	1036:1046	arg1	genes					1048:1052	hepatic lipogenesis genes	1028:1052	hepatic lipogenesis genes (AMPK, SREBP-1, HGMCoA, and ABCG8)	1028:1087	In a dose-dependent manner, JPSP consumption ameliorated the expression of hepatic lipogenesis genes (AMPK, SREBP-1, HGMCoA, and ABCG8).
34634561	6	7	theme	lipogenesis	1036:1046	arg1	HGMCoA					1070:1075	HGMCoA	1070:1075	HGMCoA	1070:1075	In a dose-dependent manner, JPSP consumption ameliorated the expression of hepatic lipogenesis genes (AMPK, SREBP-1, HGMCoA, and ABCG8).
34634561	7	8	theme	JPSP-supplemented	1162:1178	arg1	groups					1180:1185	all JPSP-supplemented groups	1158:1185	all JPSP-supplemented groups	1158:1185	The effects on the microbial community structure were determined in all JPSP-supplemented groups; however, the HF-J10 and HF-J15 diets led to a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae) compared to the HF diet, some of which represented a reversal of increases associated with HF.
34634561	3	9	theme	C57BL/6 J	489:497	arg1	mice					504:507	Three-month-old C57BL/6 J male mice	473:507	Three-month-old C57BL/6 J male mice	473:507	Three-month-old C57BL/6 J male mice, received either a control (C, 10% of lipids as energy, n = 16) or high-fat (HF, 50% of lipids as energy, n = 64) diet for nine weeks.
34634561	1	10	theme	metabolic	197:205	arg1	syndrome					207:214	metabolic syndrome	197:214	metabolic syndrome	197:214	The consumption of a high-fat diet can cause metabolic syndrome and induces host gut microbial dysbiosis and non-alcoholic fatty liver disease (NAFLD).
34634561	6	11	theme	hepatic	1028:1034	arg1	ABCG8					1082:1086	ABCG8	1082:1086	ABCG8	1082:1086	In a dose-dependent manner, JPSP consumption ameliorated the expression of hepatic lipogenesis genes (AMPK, SREBP-1, HGMCoA, and ABCG8).
34634561	6	11	theme	hepatic	1028:1034	arg1	SREBP-1					1061:1067	SREBP-1	1061:1067	SREBP-1	1061:1067	In a dose-dependent manner, JPSP consumption ameliorated the expression of hepatic lipogenesis genes (AMPK, SREBP-1, HGMCoA, and ABCG8).
34634561	6	11	theme	hepatic	1028:1034	arg1	AMPK					1055:1058	AMPK	1055:1058	AMPK	1055:1058	In a dose-dependent manner, JPSP consumption ameliorated the expression of hepatic lipogenesis genes (AMPK, SREBP-1, HGMCoA, and ABCG8).
34634561	6	11	theme	hepatic	1028:1034	arg1	genes					1048:1052	hepatic lipogenesis genes	1028:1052	hepatic lipogenesis genes (AMPK, SREBP-1, HGMCoA, and ABCG8)	1028:1087	In a dose-dependent manner, JPSP consumption ameliorated the expression of hepatic lipogenesis genes (AMPK, SREBP-1, HGMCoA, and ABCG8).
34634561	6	11	theme	hepatic	1028:1034	arg1	HGMCoA					1070:1075	HGMCoA	1070:1075	HGMCoA	1070:1075	In a dose-dependent manner, JPSP consumption ameliorated the expression of hepatic lipogenesis genes (AMPK, SREBP-1, HGMCoA, and ABCG8).
34634561	0	12	theme	diet-fed	137:144	arg1	mice					146:149	high-fat diet-fed mice	128:149	high-fat diet-fed mice	128:149	Jaboticaba (Myrciaria jaboticaba) powder consumption improves the metabolic profile and regulates gut microbiome composition in high-fat diet-fed mice.
34634561	3	13	theme	lipids	547:552	arg1	lipids					547:552	lipids	547:552	lipids as energy	547:562	Three-month-old C57BL/6 J male mice, received either a control (C, 10% of lipids as energy, n = 16) or high-fat (HF, 50% of lipids as energy, n = 64) diet for nine weeks.
34634561	3	13	theme	lipids	547:552	arg1	C					537:537	C	537:537	C	537:537	Three-month-old C57BL/6 J male mice, received either a control (C, 10% of lipids as energy, n = 16) or high-fat (HF, 50% of lipids as energy, n = 64) diet for nine weeks.
34634561	3	13	theme	lipids	547:552	arg1	%					542:542	10%	540:542	10% of lipids as energy	540:562	Three-month-old C57BL/6 J male mice, received either a control (C, 10% of lipids as energy, n = 16) or high-fat (HF, 50% of lipids as energy, n = 64) diet for nine weeks.
34634561	4	14	dep	groups	691:696	arg1	n = 16					699:704	n = 16	699:704	n = 16	699:704	The HF mice were randomly subdivided into four groups (n = 16 in each group), three of which (HF-J5, HF-J10, and HF-J15) were supplemented with dietary JPSP for four weeks (5%, 10%, and 15%, respectively).
34634561	7	15	from	depletion	1242:1250	arg1	species					1259:1265	the species	1255:1265	the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae)	1255:1452	The effects on the microbial community structure were determined in all JPSP-supplemented groups; however, the HF-J10 and HF-J15 diets led to a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae) compared to the HF diet, some of which represented a reversal of increases associated with HF.
34634561	4	16	dep	weeks	810:814	arg1	%					832:832	15%	830:832	15%	830:832	The HF mice were randomly subdivided into four groups (n = 16 in each group), three of which (HF-J5, HF-J10, and HF-J15) were supplemented with dietary JPSP for four weeks (5%, 10%, and 15%, respectively).
34634561	4	16	dep	weeks	810:814	arg1	%					818:818	5%	817:818	5%	817:818	The HF mice were randomly subdivided into four groups (n = 16 in each group), three of which (HF-J5, HF-J10, and HF-J15) were supplemented with dietary JPSP for four weeks (5%, 10%, and 15%, respectively).
34634561	4	16	dep	weeks	810:814	arg1	%					823:823	10%	821:823	10%	821:823	The HF mice were randomly subdivided into four groups (n = 16 in each group), three of which (HF-J5, HF-J10, and HF-J15) were supplemented with dietary JPSP for four weeks (5%, 10%, and 15%, respectively).
34634561	8	17	theme	Lachnospiraceae	1553:1567	arg1	families					1592:1599	The Lachnospiraceae and Enterobacteriaceae families	1549:1599	families	1592:1599	The Lachnospiraceae and Enterobacteriaceae families and the Parabacteroides, Sutterella, Allobaculum, and Akkermansia genera were enriched more in the HF-J10 and HF-J15 groups than in the HF group.
34634561	7	18	theme	community	1119:1127	arg1	structure					1129:1137	the microbial community structure	1105:1137	the microbial community structure	1105:1137	The effects on the microbial community structure were determined in all JPSP-supplemented groups; however, the HF-J10 and HF-J15 diets led to a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae) compared to the HF diet, some of which represented a reversal of increases associated with HF.
34634561	9	19	contain	had	1827:1829	arg1	consumption					1767:1777	JPSP consumption	1762:1777	JPSP consumption	1762:1777	In conclusion, JPSP consumption improved obesity-related metabolic profiles and had a strong impact on the microbial community structure, thereby reversing NAFLD and decreasing its severity.
34634561	9	19	contain	had	1827:1829	arg2	impact					1840:1845	a strong impact	1831:1845	a strong impact	1831:1845	In conclusion, JPSP consumption improved obesity-related metabolic profiles and had a strong impact on the microbial community structure, thereby reversing NAFLD and decreasing its severity.
34634561	8	20	theme	HF	1737:1738	arg1	group					1740:1744	the HF group	1733:1744	the HF group	1733:1744	The Lachnospiraceae and Enterobacteriaceae families and the Parabacteroides, Sutterella, Allobaculum, and Akkermansia genera were enriched more in the HF-J10 and HF-J15 groups than in the HF group.
34634561	1	21	theme	gut	233:235	arg1	dysbiosis					247:255	host gut microbial dysbiosis	228:255	host gut microbial dysbiosis	228:255	The consumption of a high-fat diet can cause metabolic syndrome and induces host gut microbial dysbiosis and non-alcoholic fatty liver disease (NAFLD).
34634561	8	22	theme	Enterobacteriaceae	1573:1590	arg1	families					1592:1599	The Lachnospiraceae and Enterobacteriaceae families	1549:1599	families	1592:1599	The Lachnospiraceae and Enterobacteriaceae families and the Parabacteroides, Sutterella, Allobaculum, and Akkermansia genera were enriched more in the HF-J10 and HF-J15 groups than in the HF group.
34634561	6	23	theme	JPSP	981:984	arg1	consumption					986:996	JPSP consumption	981:996	JPSP consumption	981:996	In a dose-dependent manner, JPSP consumption ameliorated the expression of hepatic lipogenesis genes (AMPK, SREBP-1, HGMCoA, and ABCG8).
34634561	5	24	theme	JPSP	890:893	arg1	consumption					895:905	JPSP consumption	890:905	JPSP consumption	890:905	In addition to attenuating weight gain, JPSP consumption improved dyslipidemia and insulin resistance.
34634561	9	25	theme	obesity-related	1788:1802	arg1	profiles					1814:1821	obesity-related metabolic profiles	1788:1821	obesity-related metabolic profiles	1788:1821	In conclusion, JPSP consumption improved obesity-related metabolic profiles and had a strong impact on the microbial community structure, thereby reversing NAFLD and decreasing its severity.
34634561	4	26	theme	HF	648:649	arg1	mice					651:654	The HF mice	644:654	The HF mice	644:654	The HF mice were randomly subdivided into four groups (n = 16 in each group), three of which (HF-J5, HF-J10, and HF-J15) were supplemented with dietary JPSP for four weeks (5%, 10%, and 15%, respectively).
34634561	8	27	theme	Sutterella	1626:1635	arg1	genera					1667:1672	the Parabacteroides, Sutterella, Allobaculum, and Akkermansia genera	1605:1672	the Parabacteroides, Sutterella, Allobaculum, and Akkermansia genera	1605:1672	The Lachnospiraceae and Enterobacteriaceae families and the Parabacteroides, Sutterella, Allobaculum, and Akkermansia genera were enriched more in the HF-J10 and HF-J15 groups than in the HF group.
34634561	2	28	from	health	439:444	arg1	model					457:461	a mouse model	449:461	a mouse model of NAFLD	449:470	We evaluated the effect of polyphenol-rich jaboticaba peel and seed powder (JPSP) on the gut microbial community composition and liver health in a mouse model of NAFLD.
34634561	0	29	theme	microbiome	102:111	arg1	composition					113:123	gut microbiome composition	98:123	gut microbiome composition in high-fat diet-fed mice	98:149	Jaboticaba (Myrciaria jaboticaba) powder consumption improves the metabolic profile and regulates gut microbiome composition in high-fat diet-fed mice.
34634561	8	30	theme	Allobaculum	1638:1648	arg1	genera					1667:1672	the Parabacteroides, Sutterella, Allobaculum, and Akkermansia genera	1605:1672	the Parabacteroides, Sutterella, Allobaculum, and Akkermansia genera	1605:1672	The Lachnospiraceae and Enterobacteriaceae families and the Parabacteroides, Sutterella, Allobaculum, and Akkermansia genera were enriched more in the HF-J10 and HF-J15 groups than in the HF group.
34634561	2	31	theme	liver	433:437	arg1	health					439:444	liver health	433:444	liver health	433:444	We evaluated the effect of polyphenol-rich jaboticaba peel and seed powder (JPSP) on the gut microbial community composition and liver health in a mouse model of NAFLD.
34634561	9	32	theme	strong	1833:1838	arg1	impact					1840:1845	a strong impact	1831:1845	a strong impact	1831:1845	In conclusion, JPSP consumption improved obesity-related metabolic profiles and had a strong impact on the microbial community structure, thereby reversing NAFLD and decreasing its severity.
34634561	0	33	theme	Jaboticaba	0:9	arg1	consumption					41:51	Jaboticaba (Myrciaria jaboticaba) powder consumption	0:51	Jaboticaba (Myrciaria jaboticaba) powder consumption	0:51	Jaboticaba (Myrciaria jaboticaba) powder consumption improves the metabolic profile and regulates gut microbiome composition in high-fat diet-fed mice.
34634561	1	34	theme	liver	281:285	arg1	NAFLD					296:300	NAFLD	296:300	NAFLD	296:300	The consumption of a high-fat diet can cause metabolic syndrome and induces host gut microbial dysbiosis and non-alcoholic fatty liver disease (NAFLD).
34634561	1	34	theme	liver	281:285	arg1	disease					287:293	non-alcoholic fatty liver disease	261:293	non-alcoholic fatty liver disease (NAFLD)	261:301	The consumption of a high-fat diet can cause metabolic syndrome and induces host gut microbial dysbiosis and non-alcoholic fatty liver disease (NAFLD).
34634561	2	35	theme	microbial	397:405	arg1	composition					417:427	gut microbial community composition	393:427	gut microbial community composition	393:427	We evaluated the effect of polyphenol-rich jaboticaba peel and seed powder (JPSP) on the gut microbial community composition and liver health in a mouse model of NAFLD.
34634561	0	36	theme	Myrciaria	12:20	arg1	consumption					41:51	Jaboticaba (Myrciaria jaboticaba) powder consumption	0:51	Jaboticaba (Myrciaria jaboticaba) powder consumption	0:51	Jaboticaba (Myrciaria jaboticaba) powder consumption improves the metabolic profile and regulates gut microbiome composition in high-fat diet-fed mice.
34634561	2	37	theme	mouse	451:455	arg1	model					457:461	a mouse model	449:461	a mouse model of NAFLD	449:470	We evaluated the effect of polyphenol-rich jaboticaba peel and seed powder (JPSP) on the gut microbial community composition and liver health in a mouse model of NAFLD.
34634561	9	38	theme	microbial	1854:1862	arg1	structure					1874:1882	the microbial community structure	1850:1882	the microbial community structure	1850:1882	In conclusion, JPSP consumption improved obesity-related metabolic profiles and had a strong impact on the microbial community structure, thereby reversing NAFLD and decreasing its severity.
34634561	7	39	theme	bacterial	1279:1287	arg1	families					1289:1296	numerous bacterial families	1270:1296	numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae)	1270:1452	The effects on the microbial community structure were determined in all JPSP-supplemented groups; however, the HF-J10 and HF-J15 diets led to a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae) compared to the HF diet, some of which represented a reversal of increases associated with HF.
34634561	7	39	theme	bacterial	1279:1287	arg1	Peptostreptococcaceae					1410:1430	Peptostreptococcaceae	1410:1430	Peptostreptococcaceae	1410:1430	The effects on the microbial community structure were determined in all JPSP-supplemented groups; however, the HF-J10 and HF-J15 diets led to a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae) compared to the HF diet, some of which represented a reversal of increases associated with HF.
34634561	7	39	theme	bacterial	1279:1287	arg1	Clostridiaceae					1358:1371	Clostridiaceae	1358:1371	Clostridiaceae	1358:1371	The effects on the microbial community structure were determined in all JPSP-supplemented groups; however, the HF-J10 and HF-J15 diets led to a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae) compared to the HF diet, some of which represented a reversal of increases associated with HF.
34634561	7	39	theme	bacterial	1279:1287	arg1	Ruminococcaceae					1437:1451	Ruminococcaceae	1437:1451	Ruminococcaceae	1437:1451	The effects on the microbial community structure were determined in all JPSP-supplemented groups; however, the HF-J10 and HF-J15 diets led to a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae) compared to the HF diet, some of which represented a reversal of increases associated with HF.
34634561	7	39	theme	bacterial	1279:1287	arg1	Mogibacteriaceae					1319:1334	Mogibacteriaceae	1319:1334	Mogibacteriaceae	1319:1334	The effects on the microbial community structure were determined in all JPSP-supplemented groups; however, the HF-J10 and HF-J15 diets led to a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae) compared to the HF diet, some of which represented a reversal of increases associated with HF.
34634561	7	39	theme	bacterial	1279:1287	arg1	Peptococcaceae					1394:1407	Peptococcaceae	1394:1407	Peptococcaceae	1394:1407	The effects on the microbial community structure were determined in all JPSP-supplemented groups; however, the HF-J10 and HF-J15 diets led to a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae) compared to the HF diet, some of which represented a reversal of increases associated with HF.
34634561	2	40	theme	polyphenol-rich	331:345	arg1	peel					358:361	polyphenol-rich jaboticaba peel	331:361	polyphenol-rich jaboticaba peel	331:361	We evaluated the effect of polyphenol-rich jaboticaba peel and seed powder (JPSP) on the gut microbial community composition and liver health in a mouse model of NAFLD.
34634561	3	41	theme	male	499:502	arg1	mice					504:507	Three-month-old C57BL/6 J male mice	473:507	Three-month-old C57BL/6 J male mice	473:507	Three-month-old C57BL/6 J male mice, received either a control (C, 10% of lipids as energy, n = 16) or high-fat (HF, 50% of lipids as energy, n = 64) diet for nine weeks.
34634561	3	42	dep	high-fat	576:583	arg1	n = 64					615:620	n = 64	615:620	n = 64	615:620	Three-month-old C57BL/6 J male mice, received either a control (C, 10% of lipids as energy, n = 16) or high-fat (HF, 50% of lipids as energy, n = 64) diet for nine weeks.
34634561	3	42	dep	high-fat	576:583	arg1	lipids					597:602	lipids	597:602	lipids as energy	597:612	Three-month-old C57BL/6 J male mice, received either a control (C, 10% of lipids as energy, n = 16) or high-fat (HF, 50% of lipids as energy, n = 64) diet for nine weeks.
34634561	3	42	dep	high-fat	576:583	arg1	HF					586:587	HF	586:587	HF	586:587	Three-month-old C57BL/6 J male mice, received either a control (C, 10% of lipids as energy, n = 16) or high-fat (HF, 50% of lipids as energy, n = 64) diet for nine weeks.
34634561	3	42	dep	high-fat	576:583	arg1	%					592:592	50%	590:592	50% of lipids as energy	590:612	Three-month-old C57BL/6 J male mice, received either a control (C, 10% of lipids as energy, n = 16) or high-fat (HF, 50% of lipids as energy, n = 64) diet for nine weeks.
34634561	7	43	from	effects	1094:1100	arg1	structure					1129:1137	the microbial community structure	1105:1137	the microbial community structure	1105:1137	The effects on the microbial community structure were determined in all JPSP-supplemented groups; however, the HF-J10 and HF-J15 diets led to a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae) compared to the HF diet, some of which represented a reversal of increases associated with HF.
34634561	2	44	theme	peel	358:361	arg1	effect					321:326	the effect	317:326	the effect of polyphenol-rich jaboticaba peel and seed powder (JPSP) on the gut microbial community composition and liver health in a mouse model of NAFLD	317:470	We evaluated the effect of polyphenol-rich jaboticaba peel and seed powder (JPSP) on the gut microbial community composition and liver health in a mouse model of NAFLD.
34634561	6	45	theme	genes	1048:1052	arg1	expression					1014:1023	the expression	1010:1023	the expression of hepatic lipogenesis genes (AMPK, SREBP-1, HGMCoA, and ABCG8)	1010:1087	In a dose-dependent manner, JPSP consumption ameliorated the expression of hepatic lipogenesis genes (AMPK, SREBP-1, HGMCoA, and ABCG8).
34634561	2	46	from	effect	321:326	arg1	composition					417:427	gut microbial community composition	393:427	gut microbial community composition	393:427	We evaluated the effect of polyphenol-rich jaboticaba peel and seed powder (JPSP) on the gut microbial community composition and liver health in a mouse model of NAFLD.
34634561	2	46	from	effect	321:326	arg1	health					439:444	liver health	433:444	liver health	433:444	We evaluated the effect of polyphenol-rich jaboticaba peel and seed powder (JPSP) on the gut microbial community composition and liver health in a mouse model of NAFLD.
34634561	3	47	theme	lipids	597:602	arg1	lipids					597:602	lipids	597:602	lipids as energy	597:612	Three-month-old C57BL/6 J male mice, received either a control (C, 10% of lipids as energy, n = 16) or high-fat (HF, 50% of lipids as energy, n = 64) diet for nine weeks.
34634561	3	47	theme	lipids	597:602	arg1	HF					586:587	HF	586:587	HF	586:587	Three-month-old C57BL/6 J male mice, received either a control (C, 10% of lipids as energy, n = 16) or high-fat (HF, 50% of lipids as energy, n = 64) diet for nine weeks.
34634561	3	47	theme	lipids	597:602	arg1	%					592:592	50%	590:592	50% of lipids as energy	590:612	Three-month-old C57BL/6 J male mice, received either a control (C, 10% of lipids as energy, n = 16) or high-fat (HF, 50% of lipids as energy, n = 64) diet for nine weeks.
34634561	1	48	theme	non-alcoholic	261:273	arg1	NAFLD					296:300	NAFLD	296:300	NAFLD	296:300	The consumption of a high-fat diet can cause metabolic syndrome and induces host gut microbial dysbiosis and non-alcoholic fatty liver disease (NAFLD).
34634561	1	48	theme	non-alcoholic	261:273	arg1	disease					287:293	non-alcoholic fatty liver disease	261:293	non-alcoholic fatty liver disease (NAFLD)	261:301	The consumption of a high-fat diet can cause metabolic syndrome and induces host gut microbial dysbiosis and non-alcoholic fatty liver disease (NAFLD).
34634561	6	49	theme	dose-dependent	958:971	arg1	manner					973:978	a dose-dependent manner	956:978	a dose-dependent manner	956:978	In a dose-dependent manner, JPSP consumption ameliorated the expression of hepatic lipogenesis genes (AMPK, SREBP-1, HGMCoA, and ABCG8).
34634561	7	50	theme	HF-J15	1212:1217	arg1	diets					1219:1223	the HF-J10 and HF-J15 diets	1197:1223	diets	1219:1223	The effects on the microbial community structure were determined in all JPSP-supplemented groups; however, the HF-J10 and HF-J15 diets led to a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae) compared to the HF diet, some of which represented a reversal of increases associated with HF.
34634561	8	51	theme	HF-J10	1700:1705	arg1	groups					1718:1723	the HF-J10 and HF-J15 groups	1696:1723	groups	1718:1723	The Lachnospiraceae and Enterobacteriaceae families and the Parabacteroides, Sutterella, Allobaculum, and Akkermansia genera were enriched more in the HF-J10 and HF-J15 groups than in the HF group.
34634561	1	52	theme	diet	182:185	arg1	consumption					156:166	The consumption	152:166	The consumption of a high-fat diet	152:185	The consumption of a high-fat diet can cause metabolic syndrome and induces host gut microbial dysbiosis and non-alcoholic fatty liver disease (NAFLD).
34634561	0	53	theme	high-fat	128:135	arg1	mice					146:149	high-fat diet-fed mice	128:149	high-fat diet-fed mice	128:149	Jaboticaba (Myrciaria jaboticaba) powder consumption improves the metabolic profile and regulates gut microbiome composition in high-fat diet-fed mice.
34634561	2	54	theme	seed	367:370	arg1	JPSP					380:383	JPSP	380:383	JPSP	380:383	We evaluated the effect of polyphenol-rich jaboticaba peel and seed powder (JPSP) on the gut microbial community composition and liver health in a mouse model of NAFLD.
34634561	2	54	theme	seed	367:370	arg1	powder					372:377	seed powder	367:377	seed powder (JPSP)	367:384	We evaluated the effect of polyphenol-rich jaboticaba peel and seed powder (JPSP) on the gut microbial community composition and liver health in a mouse model of NAFLD.
34634561	4	55	from	n = 16	699:704	arg1	group					714:718	each group	709:718	each group	709:718	The HF mice were randomly subdivided into four groups (n = 16 in each group), three of which (HF-J5, HF-J10, and HF-J15) were supplemented with dietary JPSP for four weeks (5%, 10%, and 15%, respectively).
34634561	8	56	theme	HF-J15	1711:1716	arg1	groups					1718:1723	the HF-J10 and HF-J15 groups	1696:1723	groups	1718:1723	The Lachnospiraceae and Enterobacteriaceae families and the Parabacteroides, Sutterella, Allobaculum, and Akkermansia genera were enriched more in the HF-J10 and HF-J15 groups than in the HF group.
34634561	0	57	from	composition	113:123	arg1	mice					146:149	high-fat diet-fed mice	128:149	high-fat diet-fed mice	128:149	Jaboticaba (Myrciaria jaboticaba) powder consumption improves the metabolic profile and regulates gut microbiome composition in high-fat diet-fed mice.
34634561	9	58	theme	JPSP	1762:1765	arg1	consumption					1767:1777	JPSP consumption	1762:1777	JPSP consumption	1762:1777	In conclusion, JPSP consumption improved obesity-related metabolic profiles and had a strong impact on the microbial community structure, thereby reversing NAFLD and decreasing its severity.
34634561	0	59	theme	metabolic	66:74	arg1	profile					76:82	the metabolic profile	62:82	the metabolic profile	62:82	Jaboticaba (Myrciaria jaboticaba) powder consumption improves the metabolic profile and regulates gut microbiome composition in high-fat diet-fed mice.
34634561	3	60	dep	control	528:534	arg1	lipids					547:552	lipids	547:552	lipids as energy	547:562	Three-month-old C57BL/6 J male mice, received either a control (C, 10% of lipids as energy, n = 16) or high-fat (HF, 50% of lipids as energy, n = 64) diet for nine weeks.
34634561	3	60	dep	control	528:534	arg1	C					537:537	C	537:537	C	537:537	Three-month-old C57BL/6 J male mice, received either a control (C, 10% of lipids as energy, n = 16) or high-fat (HF, 50% of lipids as energy, n = 64) diet for nine weeks.
34634561	3	60	dep	control	528:534	arg1	%					542:542	10%	540:542	10% of lipids as energy	540:562	Three-month-old C57BL/6 J male mice, received either a control (C, 10% of lipids as energy, n = 16) or high-fat (HF, 50% of lipids as energy, n = 64) diet for nine weeks.
34634561	3	60	dep	control	528:534	arg1	n = 16					565:570	n = 16	565:570	n = 16	565:570	Three-month-old C57BL/6 J male mice, received either a control (C, 10% of lipids as energy, n = 16) or high-fat (HF, 50% of lipids as energy, n = 64) diet for nine weeks.
34634561	7	61	theme	increases	1519:1527	arg1	reversal					1507:1514	a reversal	1505:1514	a reversal of increases associated with HF	1505:1546	The effects on the microbial community structure were determined in all JPSP-supplemented groups; however, the HF-J10 and HF-J15 diets led to a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae) compared to the HF diet, some of which represented a reversal of increases associated with HF.
34634561	2	62	theme	NAFLD	466:470	arg1	model					457:461	a mouse model	449:461	a mouse model of NAFLD	449:470	We evaluated the effect of polyphenol-rich jaboticaba peel and seed powder (JPSP) on the gut microbial community composition and liver health in a mouse model of NAFLD.
34634561	2	63	from	composition	417:427	arg1	model					457:461	a mouse model	449:461	a mouse model of NAFLD	449:470	We evaluated the effect of polyphenol-rich jaboticaba peel and seed powder (JPSP) on the gut microbial community composition and liver health in a mouse model of NAFLD.
34634561	7	64	theme	microbial	1109:1117	arg1	structure					1129:1137	the microbial community structure	1105:1137	the microbial community structure	1105:1137	The effects on the microbial community structure were determined in all JPSP-supplemented groups; however, the HF-J10 and HF-J15 diets led to a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae) compared to the HF diet, some of which represented a reversal of increases associated with HF.
34634561	9	65	theme	metabolic	1804:1812	arg1	profiles					1814:1821	obesity-related metabolic profiles	1788:1821	obesity-related metabolic profiles	1788:1821	In conclusion, JPSP consumption improved obesity-related metabolic profiles and had a strong impact on the microbial community structure, thereby reversing NAFLD and decreasing its severity.
34634561	0	66	theme	gut	98:100	arg1	composition					113:123	gut microbiome composition	98:123	gut microbiome composition in high-fat diet-fed mice	98:149	Jaboticaba (Myrciaria jaboticaba) powder consumption improves the metabolic profile and regulates gut microbiome composition in high-fat diet-fed mice.
34634561	5	67	theme	weight	877:882	arg1	gain					884:887	weight gain	877:887	weight gain	877:887	In addition to attenuating weight gain, JPSP consumption improved dyslipidemia and insulin resistance.
34634561	2	68	dep	composition	417:427	arg1	the					389:391	the	389:391	the	389:391	We evaluated the effect of polyphenol-rich jaboticaba peel and seed powder (JPSP) on the gut microbial community composition and liver health in a mouse model of NAFLD.
34634561	4	69	theme	dietary	788:794	arg1	JPSP					796:799	dietary JPSP	788:799	dietary JPSP	788:799	The HF mice were randomly subdivided into four groups (n = 16 in each group), three of which (HF-J5, HF-J10, and HF-J15) were supplemented with dietary JPSP for four weeks (5%, 10%, and 15%, respectively).
34634561	1	70	theme	host	228:231	arg1	dysbiosis					247:255	host gut microbial dysbiosis	228:255	host gut microbial dysbiosis	228:255	The consumption of a high-fat diet can cause metabolic syndrome and induces host gut microbial dysbiosis and non-alcoholic fatty liver disease (NAFLD).
34634561	2	71	theme	powder	372:377	arg1	effect					321:326	the effect	317:326	the effect of polyphenol-rich jaboticaba peel and seed powder (JPSP) on the gut microbial community composition and liver health in a mouse model of NAFLD	317:470	We evaluated the effect of polyphenol-rich jaboticaba peel and seed powder (JPSP) on the gut microbial community composition and liver health in a mouse model of NAFLD.
34634561	0	72	theme	jaboticaba	22:31	arg1	consumption					41:51	Jaboticaba (Myrciaria jaboticaba) powder consumption	0:51	Jaboticaba (Myrciaria jaboticaba) powder consumption	0:51	Jaboticaba (Myrciaria jaboticaba) powder consumption improves the metabolic profile and regulates gut microbiome composition in high-fat diet-fed mice.
34634561	7	73	theme	HF	1470:1471	arg1	diet					1473:1476	the HF diet	1466:1476	the HF diet	1466:1476	The effects on the microbial community structure were determined in all JPSP-supplemented groups; however, the HF-J10 and HF-J15 diets led to a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae) compared to the HF diet, some of which represented a reversal of increases associated with HF.
34634561	2	74	theme	community	407:415	arg1	composition					417:427	gut microbial community composition	393:427	gut microbial community composition	393:427	We evaluated the effect of polyphenol-rich jaboticaba peel and seed powder (JPSP) on the gut microbial community composition and liver health in a mouse model of NAFLD.
34634561	3	75	theme	high-fat	576:583	arg1	diet					623:626	high-fat (HF, 50% of lipids as energy, n = 64) diet	576:626	high-fat (HF, 50% of lipids as energy, n = 64) diet for nine weeks	576:641	Three-month-old C57BL/6 J male mice, received either a control (C, 10% of lipids as energy, n = 16) or high-fat (HF, 50% of lipids as energy, n = 64) diet for nine weeks.
34634561	2	76	theme	gut	393:395	arg1	composition					417:427	gut microbial community composition	393:427	gut microbial community composition	393:427	We evaluated the effect of polyphenol-rich jaboticaba peel and seed powder (JPSP) on the gut microbial community composition and liver health in a mouse model of NAFLD.
34634561	0	77	theme	powder	34:39	arg1	consumption					41:51	Jaboticaba (Myrciaria jaboticaba) powder consumption	0:51	Jaboticaba (Myrciaria jaboticaba) powder consumption	0:51	Jaboticaba (Myrciaria jaboticaba) powder consumption improves the metabolic profile and regulates gut microbiome composition in high-fat diet-fed mice.
34634561	9	78	theme	community	1864:1872	arg1	structure					1874:1882	the microbial community structure	1850:1882	the microbial community structure	1850:1882	In conclusion, JPSP consumption improved obesity-related metabolic profiles and had a strong impact on the microbial community structure, thereby reversing NAFLD and decreasing its severity.
34634561	4	79	theme	groups	691:696	arg1	groups					691:696	four groups	686:696	four groups (n = 16 in each group)	686:719	The HF mice were randomly subdivided into four groups (n = 16 in each group), three of which (HF-J5, HF-J10, and HF-J15) were supplemented with dietary JPSP for four weeks (5%, 10%, and 15%, respectively).
34634561	4	79	theme	groups	691:696	arg1	three					722:726	three	722:726	three	722:726	The HF mice were randomly subdivided into four groups (n = 16 in each group), three of which (HF-J5, HF-J10, and HF-J15) were supplemented with dietary JPSP for four weeks (5%, 10%, and 15%, respectively).
34634561	5	80	theme	insulin	933:939	arg1	resistance					941:950	insulin resistance	933:950	insulin resistance	933:950	In addition to attenuating weight gain, JPSP consumption improved dyslipidemia and insulin resistance.
34634561	7	81	theme	families	1289:1296	arg1	species					1259:1265	the species	1255:1265	the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae)	1255:1452	The effects on the microbial community structure were determined in all JPSP-supplemented groups; however, the HF-J10 and HF-J15 diets led to a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae) compared to the HF diet, some of which represented a reversal of increases associated with HF.
34634561	4	82	dep	supplemented	770:781	arg1	HF-J10					745:750	HF-J10	745:750	HF-J10	745:750	The HF mice were randomly subdivided into four groups (n = 16 in each group), three of which (HF-J5, HF-J10, and HF-J15) were supplemented with dietary JPSP for four weeks (5%, 10%, and 15%, respectively).
34634561	4	82	dep	supplemented	770:781	arg1	HF-J15					757:762	HF-J15	757:762	HF-J15	757:762	The HF mice were randomly subdivided into four groups (n = 16 in each group), three of which (HF-J5, HF-J10, and HF-J15) were supplemented with dietary JPSP for four weeks (5%, 10%, and 15%, respectively).
34634561	4	82	dep	supplemented	770:781	arg1	HF-J5					738:742	HF-J5	738:742	HF-J5	738:742	The HF mice were randomly subdivided into four groups (n = 16 in each group), three of which (HF-J5, HF-J10, and HF-J15) were supplemented with dietary JPSP for four weeks (5%, 10%, and 15%, respectively).
34634561	5	83	dep	attenuating	865:875	arg1	addition					853:860	addition	853:860	addition	853:860	In addition to attenuating weight gain, JPSP consumption improved dyslipidemia and insulin resistance.
34634561	7	84	theme	numerous	1270:1277	arg1	families					1289:1296	numerous bacterial families	1270:1296	numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae)	1270:1452	The effects on the microbial community structure were determined in all JPSP-supplemented groups; however, the HF-J10 and HF-J15 diets led to a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae) compared to the HF diet, some of which represented a reversal of increases associated with HF.
34634561	7	84	theme	numerous	1270:1277	arg1	Peptostreptococcaceae					1410:1430	Peptostreptococcaceae	1410:1430	Peptostreptococcaceae	1410:1430	The effects on the microbial community structure were determined in all JPSP-supplemented groups; however, the HF-J10 and HF-J15 diets led to a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae) compared to the HF diet, some of which represented a reversal of increases associated with HF.
34634561	7	84	theme	numerous	1270:1277	arg1	Clostridiaceae					1358:1371	Clostridiaceae	1358:1371	Clostridiaceae	1358:1371	The effects on the microbial community structure were determined in all JPSP-supplemented groups; however, the HF-J10 and HF-J15 diets led to a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae) compared to the HF diet, some of which represented a reversal of increases associated with HF.
34634561	7	84	theme	numerous	1270:1277	arg1	Ruminococcaceae					1437:1451	Ruminococcaceae	1437:1451	Ruminococcaceae	1437:1451	The effects on the microbial community structure were determined in all JPSP-supplemented groups; however, the HF-J10 and HF-J15 diets led to a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae) compared to the HF diet, some of which represented a reversal of increases associated with HF.
34634561	7	84	theme	numerous	1270:1277	arg1	Mogibacteriaceae					1319:1334	Mogibacteriaceae	1319:1334	Mogibacteriaceae	1319:1334	The effects on the microbial community structure were determined in all JPSP-supplemented groups; however, the HF-J10 and HF-J15 diets led to a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae) compared to the HF diet, some of which represented a reversal of increases associated with HF.
34634561	7	84	theme	numerous	1270:1277	arg1	Peptococcaceae					1394:1407	Peptococcaceae	1394:1407	Peptococcaceae	1394:1407	The effects on the microbial community structure were determined in all JPSP-supplemented groups; however, the HF-J10 and HF-J15 diets led to a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae) compared to the HF diet, some of which represented a reversal of increases associated with HF.
34634561	7	85	dep	families	1289:1296	arg1	families					1289:1296	numerous bacterial families	1270:1296	numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae)	1270:1452	The effects on the microbial community structure were determined in all JPSP-supplemented groups; however, the HF-J10 and HF-J15 diets led to a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae) compared to the HF diet, some of which represented a reversal of increases associated with HF.
34634561	7	85	dep	families	1289:1296	arg1	Peptostreptococcaceae					1410:1430	Peptostreptococcaceae	1410:1430	Peptostreptococcaceae	1410:1430	The effects on the microbial community structure were determined in all JPSP-supplemented groups; however, the HF-J10 and HF-J15 diets led to a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae) compared to the HF diet, some of which represented a reversal of increases associated with HF.
34634561	7	85	dep	families	1289:1296	arg1	Clostridiaceae					1358:1371	Clostridiaceae	1358:1371	Clostridiaceae	1358:1371	The effects on the microbial community structure were determined in all JPSP-supplemented groups; however, the HF-J10 and HF-J15 diets led to a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae) compared to the HF diet, some of which represented a reversal of increases associated with HF.
34634561	7	85	dep	families	1289:1296	arg1	Ruminococcaceae					1437:1451	Ruminococcaceae	1437:1451	Ruminococcaceae	1437:1451	The effects on the microbial community structure were determined in all JPSP-supplemented groups; however, the HF-J10 and HF-J15 diets led to a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae) compared to the HF diet, some of which represented a reversal of increases associated with HF.
34634561	7	85	dep	families	1289:1296	arg1	Mogibacteriaceae					1319:1334	Mogibacteriaceae	1319:1334	Mogibacteriaceae	1319:1334	The effects on the microbial community structure were determined in all JPSP-supplemented groups; however, the HF-J10 and HF-J15 diets led to a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae) compared to the HF diet, some of which represented a reversal of increases associated with HF.
34634561	7	85	dep	families	1289:1296	arg1	Peptococcaceae					1394:1407	Peptococcaceae	1394:1407	Peptococcaceae	1394:1407	The effects on the microbial community structure were determined in all JPSP-supplemented groups; however, the HF-J10 and HF-J15 diets led to a drastic depletion in the species of numerous bacterial families (Bifidobacteriaceae, Mogibacteriaceae, Christensenellaceae, Clostridiaceae, Dehalobacteriaceae, Peptococcaceae, Peptostreptococcaceae, and Ruminococcaceae) compared to the HF diet, some of which represented a reversal of increases associated with HF.
34634561	6	86	dep	genes	1048:1052	arg1	ABCG8					1082:1086	ABCG8	1082:1086	ABCG8	1082:1086	In a dose-dependent manner, JPSP consumption ameliorated the expression of hepatic lipogenesis genes (AMPK, SREBP-1, HGMCoA, and ABCG8).
34634561	6	86	dep	genes	1048:1052	arg1	SREBP-1					1061:1067	SREBP-1	1061:1067	SREBP-1	1061:1067	In a dose-dependent manner, JPSP consumption ameliorated the expression of hepatic lipogenesis genes (AMPK, SREBP-1, HGMCoA, and ABCG8).
34634561	6	86	dep	genes	1048:1052	arg1	AMPK					1055:1058	AMPK	1055:1058	AMPK	1055:1058	In a dose-dependent manner, JPSP consumption ameliorated the expression of hepatic lipogenesis genes (AMPK, SREBP-1, HGMCoA, and ABCG8).
34634561	6	86	dep	genes	1048:1052	arg1	genes					1048:1052	hepatic lipogenesis genes	1028:1052	hepatic lipogenesis genes (AMPK, SREBP-1, HGMCoA, and ABCG8)	1028:1087	In a dose-dependent manner, JPSP consumption ameliorated the expression of hepatic lipogenesis genes (AMPK, SREBP-1, HGMCoA, and ABCG8).
34634561	6	86	dep	genes	1048:1052	arg1	HGMCoA					1070:1075	HGMCoA	1070:1075	HGMCoA	1070:1075	In a dose-dependent manner, JPSP consumption ameliorated the expression of hepatic lipogenesis genes (AMPK, SREBP-1, HGMCoA, and ABCG8).
34634561	1	87	theme	microbial	237:245	arg1	dysbiosis					247:255	host gut microbial dysbiosis	228:255	host gut microbial dysbiosis	228:255	The consumption of a high-fat diet can cause metabolic syndrome and induces host gut microbial dysbiosis and non-alcoholic fatty liver disease (NAFLD).
32313101	0	0	theme	standard	87:94	arg1	bioassays					96:104	standard bioassays	87:104	standard bioassays	87:104	Ultrabright fluorescent nanoscale labels for the femtomolar detection of analytes with standard bioassays.
32313101	3	1	theme	high-affinity	720:732	arg1	element					749:755	a high-affinity biorecognition element	718:755	a high-affinity biorecognition element	718:755	The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element.
32313101	5	2	theme	overall	1135:1141	arg1	times					1149:1153	overall assay times	1135:1153	overall assay times	1135:1153	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	5	3	dep	4,750-fold	981:990	arg1	to					978:979	to	978:979	to	978:979	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	5	4	from	limit	909:913	arg1	assays					965:970	fluorescence-linked immunosorbent assays	931:970	fluorescence-linked immunosorbent assays	931:970	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	1	5	theme	biological	181:190	arg1	samples					192:198	biological samples	181:198	biological samples	181:198	The detection and quantification of low-abundance molecular biomarkers in biological samples is challenging.
32313101	3	6	theme	IRDye	526:530	arg1	biotin					708:713	biotin	708:713	biotin	708:713	The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element.
32313101	3	6	theme	IRDye	526:530	arg1	fluorophores					538:549	approximately 210 IRDye 800CW fluorophores	508:549	approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore)	508:641	The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element.
32313101	3	6	theme	IRDye	526:530	arg1	nanorod					666:672	a polymer-coated gold nanorod	644:672	a polymer-coated gold nanorod acting as a plasmonic antenna	644:702	The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element.
32313101	4	7	theme	emission	762:769	arg1	wavelength					771:780	Its emission wavelength	758:780	Its emission wavelength	758:780	Its emission wavelength can be tuned over the visible and near-infrared spectral regions by modifying its size, shape and composition.
32313101	3	8	theme	biorecognition	734:747	arg1	element					749:755	a high-affinity biorecognition element	718:755	a high-affinity biorecognition element	718:755	The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element.
32313101	0	9	with	analytes	73:80	arg1	bioassays					96:104	standard bioassays	87:104	standard bioassays	87:104	Ultrabright fluorescent nanoscale labels for the femtomolar detection of analytes with standard bioassays.
32313101	4	10	theme	visible	804:810	arg1	regions					839:845	the visible and near-infrared spectral regions	800:845	the visible and near-infrared spectral regions	800:845	Its emission wavelength can be tuned over the visible and near-infrared spectral regions by modifying its size, shape and composition.
32313101	1	11	theme	low-abundance	143:155	arg1	biomarkers					167:176	low-abundance molecular biomarkers	143:176	low-abundance molecular biomarkers	143:176	The detection and quantification of low-abundance molecular biomarkers in biological samples is challenging.
32313101	5	12	theme	assay	1143:1147	arg1	times					1149:1153	overall assay times	1135:1153	overall assay times	1135:1153	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	3	13	theme	bovine	473:478	arg1	albumin					486:492	a bovine serum albumin	471:492	a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element	471:755	The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element.
32313101	5	14	theme	immunosorbent	951:963	arg1	assays					965:970	fluorescence-linked immunosorbent assays	931:970	fluorescence-linked immunosorbent assays	931:970	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	5	15	theme	fluorescence-linked	931:949	arg1	assays					965:970	fluorescence-linked immunosorbent assays	931:970	fluorescence-linked immunosorbent assays	931:970	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	3	16	theme	plasmonic	439:447	arg1	construct					449:457	The plasmonic construct	435:457	The plasmonic construct	435:457	The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element.
32313101	5	17	theme	cytokine	1243:1250	arg1	detection					1211:1219	the detection	1207:1219	the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples	1207:1323	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	0	18	theme	fluorescent	12:22	arg1	labels					34:39	fluorescent nanoscale labels	12:39	fluorescent nanoscale labels for the femtomolar detection of analytes with standard bioassays	12:104	Ultrabright fluorescent nanoscale labels for the femtomolar detection of analytes with standard bioassays.
32313101	3	19	theme	800CW	532:536	arg1	biotin					708:713	biotin	708:713	biotin	708:713	The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element.
32313101	3	19	theme	800CW	532:536	arg1	fluorophores					538:549	approximately 210 IRDye 800CW fluorophores	508:549	approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore)	508:641	The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element.
32313101	3	19	theme	800CW	532:536	arg1	nanorod					666:672	a polymer-coated gold nanorod	644:672	a polymer-coated gold nanorod acting as a plasmonic antenna	644:702	The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element.
32313101	3	20	with	scaffold	494:501	arg1	biotin					708:713	biotin	708:713	biotin	708:713	The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element.
32313101	3	20	with	scaffold	494:501	arg1	fluorophores					538:549	approximately 210 IRDye 800CW fluorophores	508:549	approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore)	508:641	The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element.
32313101	3	20	with	scaffold	494:501	arg1	nanorod					666:672	a polymer-coated gold nanorod	644:672	a polymer-coated gold nanorod acting as a plasmonic antenna	644:702	The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element.
32313101	5	21	theme	bead-based	1027:1036	arg1	immunoassays					1038:1049	multiplexed bead-based immunoassays	1015:1049	multiplexed bead-based immunoassays	1015:1049	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	5	22	from	detection	918:926	arg1	assays					965:970	fluorescence-linked immunosorbent assays	931:970	fluorescence-linked immunosorbent assays	931:970	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	2	23	theme	broad	302:306	arg1	range					308:312	a broad range	300:312	a broad range of bioassays	300:325	Here, we show that a plasmonic nanoscale construct serving as an 'add-on' label for a broad range of bioassays improves their signal-to-noise ratio and dynamic range without altering their workflow and readout devices.
32313101	4	24	theme	near-infrared	816:828	arg1	regions					839:845	the visible and near-infrared spectral regions	800:845	the visible and near-infrared spectral regions	800:845	Its emission wavelength can be tuned over the visible and near-infrared spectral regions by modifying its size, shape and composition.
32313101	1	25	from	quantification	125:138	arg1	samples					192:198	biological samples	181:198	biological samples	181:198	The detection and quantification of low-abundance molecular biomarkers in biological samples is challenging.
32313101	3	26	theme	fluorescence	559:570	arg1	intensity					572:580	a fluorescence intensity	557:580	a fluorescence intensity	557:580	The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element.
32313101	0	27	theme	nanoscale	24:32	arg1	labels					34:39	fluorescent nanoscale labels	12:39	fluorescent nanoscale labels for the femtomolar detection of analytes with standard bioassays	12:104	Ultrabright fluorescent nanoscale labels for the femtomolar detection of analytes with standard bioassays.
32313101	3	28	theme	gold	661:664	arg1	fluorophores					538:549	approximately 210 IRDye 800CW fluorophores	508:549	approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore)	508:641	The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element.
32313101	3	28	theme	gold	661:664	arg1	nanorod					666:672	a polymer-coated gold nanorod	644:672	a polymer-coated gold nanorod acting as a plasmonic antenna	644:702	The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element.
32313101	2	29	theme	signal-to-noise	342:356	arg1	ratio					358:362	their signal-to-noise ratio	336:362	their signal-to-noise ratio	336:362	Here, we show that a plasmonic nanoscale construct serving as an 'add-on' label for a broad range of bioassays improves their signal-to-noise ratio and dynamic range without altering their workflow and readout devices.
32313101	3	30	dep	6,700-fold	596:605	arg1	that					607:610	that	607:610	that	607:610	The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element.
32313101	1	31	theme	molecular	157:165	arg1	biomarkers					167:176	low-abundance molecular biomarkers	143:176	low-abundance molecular biomarkers	143:176	The detection and quantification of low-abundance molecular biomarkers in biological samples is challenging.
32313101	3	32	with	fluorophores	538:549	arg1	intensity					572:580	a fluorescence intensity	557:580	a fluorescence intensity	557:580	The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element.
32313101	2	33	theme	nanoscale	247:255	arg1	construct					257:265	a plasmonic nanoscale construct	235:265	a plasmonic nanoscale construct serving as an 'add-on' label for a broad range of bioassays	235:325	Here, we show that a plasmonic nanoscale construct serving as an 'add-on' label for a broad range of bioassays improves their signal-to-noise ratio and dynamic range without altering their workflow and readout devices.
32313101	5	34	with	compatible	999:1008	arg1	immunomicroarrays					1052:1068	immunomicroarrays	1052:1068	immunomicroarrays	1052:1068	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	5	34	with	compatible	999:1008	arg1	cytometry					1076:1084	flow cytometry	1071:1084	flow cytometry	1071:1084	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	5	34	with	compatible	999:1008	arg1	immunoassays					1038:1049	multiplexed bead-based immunoassays	1015:1049	multiplexed bead-based immunoassays	1015:1049	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	5	34	with	compatible	999:1008	arg1	methods					1110:1116	immunocytochemistry methods	1090:1116	immunocytochemistry methods	1090:1116	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	5	35	from	detection	1211:1219	arg1	samples					1317:1323	patient samples	1309:1323	patient samples	1309:1323	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	5	35	from	detection	1211:1219	arg1	fluid					1274:1278	mouse interstitial fluid	1255:1278	mouse interstitial fluid	1255:1278	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	2	36	dep	workflow	405:412	arg1	devices					426:432	devices	426:432	devices	426:432	Here, we show that a plasmonic nanoscale construct serving as an 'add-on' label for a broad range of bioassays improves their signal-to-noise ratio and dynamic range without altering their workflow and readout devices.
32313101	5	37	from	cytokine	1243:1250	arg1	fluid					1274:1278	mouse interstitial fluid	1255:1278	mouse interstitial fluid	1255:1278	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	5	38	link	fluorescence-linked	931:949	arg1	assays					965:970	fluorescence-linked immunosorbent assays	931:970	fluorescence-linked immunosorbent assays	931:970	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	3	39	theme	polymer-coated	646:659	arg1	fluorophores					538:549	approximately 210 IRDye 800CW fluorophores	508:549	approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore)	508:641	The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element.
32313101	3	39	theme	polymer-coated	646:659	arg1	nanorod					666:672	a polymer-coated gold nanorod	644:672	a polymer-coated gold nanorod acting as a plasmonic antenna	644:702	The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element.
32313101	5	40	from	samples	1317:1323	arg1	detection					1211:1219	the detection	1207:1219	the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples	1207:1323	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	5	41	theme	detection	918:926	arg1	limit					909:913	the limit	905:913	the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold	905:990	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	0	42	theme	femtomolar	49:58	arg1	detection					60:68	the femtomolar detection	45:68	the femtomolar detection of analytes with standard bioassays	45:104	Ultrabright fluorescent nanoscale labels for the femtomolar detection of analytes with standard bioassays.
32313101	5	43	theme	sample	1178:1183	arg1	volumes					1185:1191	sample volumes	1178:1191	sample volumes	1178:1191	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	2	44	dep	add-on	282:287	arg1	label					290:294	label	290:294	label for a broad range of bioassays	290:325	Here, we show that a plasmonic nanoscale construct serving as an 'add-on' label for a broad range of bioassays improves their signal-to-noise ratio and dynamic range without altering their workflow and readout devices.
32313101	5	45	theme	flow	1071:1074	arg1	cytometry					1076:1084	flow cytometry	1071:1084	flow cytometry	1071:1084	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	1	46	theme	biomarkers	167:176	arg1	quantification					125:138	quantification	125:138	quantification	125:138	The detection and quantification of low-abundance molecular biomarkers in biological samples is challenging.
32313101	1	46	theme	biomarkers	167:176	arg1	detection					111:119	detection	111:119	detection	111:119	The detection and quantification of low-abundance molecular biomarkers in biological samples is challenging.
32313101	5	47	theme	urinary	1287:1293	arg1	biomarkers					1295:1304	urinary biomarkers	1287:1304	urinary biomarkers in patient samples	1287:1323	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	4	48	theme	spectral	830:837	arg1	regions					839:845	the visible and near-infrared spectral regions	800:845	the visible and near-infrared spectral regions	800:845	Its emission wavelength can be tuned over the visible and near-infrared spectral regions by modifying its size, shape and composition.
32313101	3	49	theme	plasmonic	686:694	arg1	antenna					696:702	a plasmonic antenna	684:702	a plasmonic antenna	684:702	The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element.
32313101	3	50	theme	single	617:622	arg1	fluorophore					630:640	a single 800CW fluorophore	615:640	a single 800CW fluorophore	615:640	The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element.
32313101	2	51	theme	plasmonic	237:245	arg1	construct					257:265	a plasmonic nanoscale construct	235:265	a plasmonic nanoscale construct serving as an 'add-on' label for a broad range of bioassays	235:325	Here, we show that a plasmonic nanoscale construct serving as an 'add-on' label for a broad range of bioassays improves their signal-to-noise ratio and dynamic range without altering their workflow and readout devices.
32313101	2	52	theme	bioassays	317:325	arg1	range					308:312	a broad range	300:312	a broad range of bioassays	300:325	Here, we show that a plasmonic nanoscale construct serving as an 'add-on' label for a broad range of bioassays improves their signal-to-noise ratio and dynamic range without altering their workflow and readout devices.
32313101	1	53	from	detection	111:119	arg1	samples					192:198	biological samples	181:198	biological samples	181:198	The detection and quantification of low-abundance molecular biomarkers in biological samples is challenging.
32313101	5	54	theme	pro-inflammatory	1226:1241	arg1	cytokine					1243:1250	a pro-inflammatory cytokine	1224:1250	a pro-inflammatory cytokine in mouse interstitial fluid	1224:1278	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	5	55	from	fluid	1274:1278	arg1	detection					1211:1219	the detection	1207:1219	the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples	1207:1323	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	5	56	theme	immunocytochemistry	1090:1108	arg1	methods					1110:1116	immunocytochemistry methods	1090:1116	immunocytochemistry methods	1090:1116	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	5	57	theme	multiplexed	1015:1025	arg1	immunoassays					1038:1049	multiplexed bead-based immunoassays	1015:1049	multiplexed bead-based immunoassays	1015:1049	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	5	58	theme	biomarkers	1295:1304	arg1	detection					1211:1219	the detection	1207:1219	the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples	1207:1323	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	3	59	theme	albumin	486:492	arg1	scaffold					494:501	a bovine serum albumin scaffold	471:501	a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element	471:755	The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element.
32313101	5	60	from	assays	965:970	arg1	limit					909:913	the limit	905:913	the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold	905:990	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	3	61	theme	serum	480:484	arg1	albumin					486:492	a bovine serum albumin	471:492	a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element	471:755	The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element.
32313101	0	62	theme	analytes	73:80	arg1	detection					60:68	the femtomolar detection	45:68	the femtomolar detection of analytes with standard bioassays	45:104	Ultrabright fluorescent nanoscale labels for the femtomolar detection of analytes with standard bioassays.
32313101	3	63	theme	800CW	624:628	arg1	fluorophore					630:640	a single 800CW fluorophore	615:640	a single 800CW fluorophore	615:640	The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element.
32313101	2	64	theme	dynamic	368:374	arg1	range					376:380	dynamic range	368:380	dynamic range	368:380	Here, we show that a plasmonic nanoscale construct serving as an 'add-on' label for a broad range of bioassays improves their signal-to-noise ratio and dynamic range without altering their workflow and readout devices.
32313101	5	65	theme	patient	1309:1315	arg1	samples					1317:1323	patient samples	1309:1323	patient samples	1309:1323	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	5	66	from	biomarkers	1295:1304	arg1	samples					1317:1323	patient samples	1309:1323	patient samples	1309:1323	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	3	67	theme	fluorophore	630:640	arg1	fluorophores					538:549	approximately 210 IRDye 800CW fluorophores	508:549	approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore)	508:641	The plasmonic construct consists of a bovine serum albumin scaffold with approximately 210 IRDye 800CW fluorophores (with a fluorescence intensity approximately 6,700-fold that of a single 800CW fluorophore), a polymer-coated gold nanorod acting as a plasmonic antenna and biotin as a high-affinity biorecognition element.
32313101	1	68	dep	detection	111:119	arg1	The					107:109	The	107:109	The	107:109	The detection and quantification of low-abundance molecular biomarkers in biological samples is challenging.
32313101	5	69	theme	interstitial	1261:1272	arg1	fluid					1274:1278	mouse interstitial fluid	1255:1278	mouse interstitial fluid	1255:1278	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32313101	5	70	theme	mouse	1255:1259	arg1	fluid					1274:1278	mouse interstitial fluid	1255:1278	mouse interstitial fluid	1255:1278	It improves the limit of detection in fluorescence-linked immunosorbent assays by up to 4,750-fold and is compatible with multiplexed bead-based immunoassays, immunomicroarrays, flow cytometry and immunocytochemistry methods, and it shortens overall assay times (to 20 min) and lowers sample volumes, as shown for the detection of a pro-inflammatory cytokine in mouse interstitial fluid and of urinary biomarkers in patient samples.
32744986	10	0	theme	ignavus	1414:1420	arg1	species					1372:1378	The type species	1363:1378	The type species of the novel genus	1363:1397	The type species of the novel genus is Fundicoccus ignavus gen. nov., sp.
32744986	10	0	theme	ignavus	1414:1420	arg1	sp					1433:1434	sp	1433:1434	sp	1433:1434	The type species of the novel genus is Fundicoccus ignavus gen. nov., sp.
32744986	10	0	theme	ignavus	1414:1420	arg1	nov.					1427:1430	Fundicoccus ignavus gen. nov.	1402:1430	Fundicoccus ignavus gen. nov.	1402:1430	The type species of the novel genus is Fundicoccus ignavus gen. nov., sp.
32744986	1	1	attach	isolated	83:90	arg2	Aerococcaceae					69:81	the family Aerococcaceae	58:81	the family Aerococcaceae isolated from bulk tank milk	58:110	nov., a novel genus of the family Aerococcaceae isolated from bulk tank milk.
32744986	1	1	attach	isolated	83:90	arg1	milk					107:110	bulk tank milk	97:110	bulk tank milk	97:110	nov., a novel genus of the family Aerococcaceae isolated from bulk tank milk.
32744986	9	2	theme	family	1275:1280	arg1	Aerococcaceae					1282:1294	the family Aerococcaceae	1271:1294	the family Aerococcaceae	1271:1294	Based on phylogenetic, phylogenomic and biochemical characterizations, the isolates can be demarcated from all other genera of the family Aerococcaceae and, therefore, the novel genus Fundicoccus gen. nov. is proposed.
32744986	3	3	theme	16S	292:294	arg1	sequences					306:314	the 16S rRNA gene sequences	288:314	the 16S rRNA gene sequences	288:314	Phylogenetic analyses based on the 16S rRNA gene sequences indicated that the three strains (WS4937T, WS4759 and WS5303) constitute an independent phylogenetic lineage within the family Aerococcaceae with Facklamia hominis CCUG 36813T (93.7-94.1 %) and Eremococcus coleocola M1831/95/2T (93.5 %) as most closely related type species.
32744986	3	4	theme	CCUG	480:483	arg1	36813T					485:490	Facklamia hominis CCUG 36813T	462:490	Facklamia hominis CCUG 36813T (93.7-94.1 %)	462:504	Phylogenetic analyses based on the 16S rRNA gene sequences indicated that the three strains (WS4937T, WS4759 and WS5303) constitute an independent phylogenetic lineage within the family Aerococcaceae with Facklamia hominis CCUG 36813T (93.7-94.1 %) and Eremococcus coleocola M1831/95/2T (93.5 %) as most closely related type species.
32744986	3	4	theme	CCUG	480:483	arg1	%					503:503	93.7-94.1 %	493:503	93.7-94.1 %	493:503	Phylogenetic analyses based on the 16S rRNA gene sequences indicated that the three strains (WS4937T, WS4759 and WS5303) constitute an independent phylogenetic lineage within the family Aerococcaceae with Facklamia hominis CCUG 36813T (93.7-94.1 %) and Eremococcus coleocola M1831/95/2T (93.5 %) as most closely related type species.
32744986	10	5	theme	type	1367:1370	arg1	species					1372:1378	The type species	1363:1378	The type species of the novel genus	1363:1397	The type species of the novel genus is Fundicoccus ignavus gen. nov., sp.
32744986	10	5	theme	type	1367:1370	arg1	nov.					1427:1430	Fundicoccus ignavus gen. nov.	1402:1430	Fundicoccus ignavus gen. nov.	1402:1430	The type species of the novel genus is Fundicoccus ignavus gen. nov., sp.
32744986	6	6	theme	glutamic	823:830	arg1	acid					832:835	glutamic acid	823:835	glutamic acid	823:835	Their cell-wall peptidoglycan belongs to the A1α type (l-Lys-direct) consisting of alanine, glutamic acid and lysine.
32744986	6	7	theme	cell-wall	737:745	arg1	peptidoglycan					747:759	Their cell-wall peptidoglycan	731:759	Their cell-wall peptidoglycan	731:759	Their cell-wall peptidoglycan belongs to the A1α type (l-Lys-direct) consisting of alanine, glutamic acid and lysine.
32744986	4	8	dep	%	654:654	arg1	w/v					657:659	w/v	657:659	w/v	657:659	The unclassified strains demonstrated variable growth with 6.5 % (w/v) NaCl and tolerated pH 6.5-9.5.
32744986	9	9	theme	other	1255:1259	arg1	genera					1261:1266	all other genera	1251:1266	all other genera of the family Aerococcaceae	1251:1294	Based on phylogenetic, phylogenomic and biochemical characterizations, the isolates can be demarcated from all other genera of the family Aerococcaceae and, therefore, the novel genus Fundicoccus gen. nov. is proposed.
32744986	2	10	dep	Gram-stain-positive	132:150	arg1	facultative					172:182	facultative	172:182	facultative	172:182	Three strains of a Gram-stain-positive, catalase-negative, facultative anaerobic, and coccoid species were isolated from German bulk tank milk.
32744986	2	10	dep	Gram-stain-positive	132:150	arg1	catalase-negative					153:169	catalase-negative	153:169	catalase-negative	153:169	Three strains of a Gram-stain-positive, catalase-negative, facultative anaerobic, and coccoid species were isolated from German bulk tank milk.
32744986	4	11	theme	%	654:654	arg1	NaCl					662:665	6.5 % (w/v) NaCl	650:665	6.5 % (w/v) NaCl	650:665	The unclassified strains demonstrated variable growth with 6.5 % (w/v) NaCl and tolerated pH 6.5-9.5.
32744986	5	12	dep	39	724:725	arg1	to					721:722	to	721:722	to	721:722	Growth was observed from 12 to 39 °C.
32744986	1	13	theme	novel	43:47	arg1	genus					49:53	a novel genus	41:53	a novel genus of the family Aerococcaceae isolated from bulk tank milk	41:110	nov., a novel genus of the family Aerococcaceae isolated from bulk tank milk.
32744986	1	13	theme	novel	43:47	arg1	nov.					35:38	nov.	35:38	nov.	35:38	nov., a novel genus of the family Aerococcaceae isolated from bulk tank milk.
32744986	8	14	theme	~3.0 Mb	1135:1141	arg1	size					1127:1130	a genome size	1118:1130	a genome size of ~3.0 Mb	1118:1141	The G+C content of strain WS4937T was 37.4 mol% with a genome size of ~3.0 Mb.
32744986	9	15	theme	novel	1316:1320	arg1	nov.					1345:1348	the novel genus Fundicoccus gen. nov.	1312:1348	the novel genus Fundicoccus gen. nov.	1312:1348	Based on phylogenetic, phylogenomic and biochemical characterizations, the isolates can be demarcated from all other genera of the family Aerococcaceae and, therefore, the novel genus Fundicoccus gen. nov. is proposed.
32744986	8	16	theme	G+C	1069:1071	arg1	%					1111:1111	37.4 mol%	1103:1111	37.4 mol%	1103:1111	The G+C content of strain WS4937T was 37.4 mol% with a genome size of ~3.0 Mb.
32744986	8	16	theme	G+C	1069:1071	arg1	content					1073:1079	The G+C content	1065:1079	The G+C content of strain WS4937T	1065:1097	The G+C content of strain WS4937T was 37.4 mol% with a genome size of ~3.0 Mb.
32744986	3	17	with	Aerococcaceae	443:455	arg1	M1831/95/2T					532:542	M1831/95/2T	532:542	M1831/95/2T	532:542	Phylogenetic analyses based on the 16S rRNA gene sequences indicated that the three strains (WS4937T, WS4759 and WS5303) constitute an independent phylogenetic lineage within the family Aerococcaceae with Facklamia hominis CCUG 36813T (93.7-94.1 %) and Eremococcus coleocola M1831/95/2T (93.5 %) as most closely related type species.
32744986	3	17	with	Aerococcaceae	443:455	arg1	36813T					485:490	Facklamia hominis CCUG 36813T	462:490	Facklamia hominis CCUG 36813T (93.7-94.1 %)	462:504	Phylogenetic analyses based on the 16S rRNA gene sequences indicated that the three strains (WS4937T, WS4759 and WS5303) constitute an independent phylogenetic lineage within the family Aerococcaceae with Facklamia hominis CCUG 36813T (93.7-94.1 %) and Eremococcus coleocola M1831/95/2T (93.5 %) as most closely related type species.
32744986	3	17	with	Aerococcaceae	443:455	arg1	%					503:503	93.7-94.1 %	493:503	93.7-94.1 %	493:503	Phylogenetic analyses based on the 16S rRNA gene sequences indicated that the three strains (WS4937T, WS4759 and WS5303) constitute an independent phylogenetic lineage within the family Aerococcaceae with Facklamia hominis CCUG 36813T (93.7-94.1 %) and Eremococcus coleocola M1831/95/2T (93.5 %) as most closely related type species.
32744986	3	17	with	Aerococcaceae	443:455	arg1	%					550:550	93.5 %	545:550	93.5 %	545:550	Phylogenetic analyses based on the 16S rRNA gene sequences indicated that the three strains (WS4937T, WS4759 and WS5303) constitute an independent phylogenetic lineage within the family Aerococcaceae with Facklamia hominis CCUG 36813T (93.7-94.1 %) and Eremococcus coleocola M1831/95/2T (93.5 %) as most closely related type species.
32744986	7	18	theme	predominant	853:863	arg1	C16 					882:885	C16 	882:885	C16 	882:885	The predominant fatty acids were C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c and in the polar lipids profile three glycolipids, a phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol were found.
32744986	7	18	theme	predominant	853:863	arg1	acids					871:875	The predominant fatty acids	849:875	The predominant fatty acids	849:875	The predominant fatty acids were C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c and in the polar lipids profile three glycolipids, a phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol were found.
32744986	2	19	theme	bulk	241:244	arg1	milk					251:254	German bulk tank milk	234:254	German bulk tank milk	234:254	Three strains of a Gram-stain-positive, catalase-negative, facultative anaerobic, and coccoid species were isolated from German bulk tank milk.
32744986	3	20	theme	independent	392:402	arg1	lineage					417:423	an independent phylogenetic lineage	389:423	an independent phylogenetic lineage within the family Aerococcaceae with Facklamia hominis CCUG 36813T (93.7-94.1 %) and Eremococcus coleocola M1831/95/2T (93.5 %) as most closely related type species	389:588	Phylogenetic analyses based on the 16S rRNA gene sequences indicated that the three strains (WS4937T, WS4759 and WS5303) constitute an independent phylogenetic lineage within the family Aerococcaceae with Facklamia hominis CCUG 36813T (93.7-94.1 %) and Eremococcus coleocola M1831/95/2T (93.5 %) as most closely related type species.
32744986	0	21	theme	ignavus	12:18	arg1	nov.					25:28	Fundicoccus ignavus gen. nov.	0:28	Fundicoccus ignavus gen. nov.	0:28	Fundicoccus ignavus gen. nov., sp.
32744986	10	22	theme	Fundicoccus	1402:1412	arg1	species					1372:1378	The type species	1363:1378	The type species of the novel genus	1363:1397	The type species of the novel genus is Fundicoccus ignavus gen. nov., sp.
32744986	10	22	theme	Fundicoccus	1402:1412	arg1	sp					1433:1434	sp	1433:1434	sp	1433:1434	The type species of the novel genus is Fundicoccus ignavus gen. nov., sp.
32744986	10	22	theme	Fundicoccus	1402:1412	arg1	nov.					1427:1430	Fundicoccus ignavus gen. nov.	1402:1430	Fundicoccus ignavus gen. nov.	1402:1430	The type species of the novel genus is Fundicoccus ignavus gen. nov., sp.
32744986	7	23	theme	fatty	865:869	arg1	C16 					882:885	C16 	882:885	C16 	882:885	The predominant fatty acids were C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c and in the polar lipids profile three glycolipids, a phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol were found.
32744986	7	23	theme	fatty	865:869	arg1	acids					871:875	The predominant fatty acids	849:875	The predominant fatty acids	849:875	The predominant fatty acids were C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c and in the polar lipids profile three glycolipids, a phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol were found.
32744986	4	24	theme	pH	681:682	arg1	6.5-9.5					684:690	tolerated pH 6.5-9.5	671:690	tolerated pH 6.5-9.5	671:690	The unclassified strains demonstrated variable growth with 6.5 % (w/v) NaCl and tolerated pH 6.5-9.5.
32744986	4	25	theme	unclassified	595:606	arg1	strains					608:614	The unclassified strains	591:614	The unclassified strains	591:614	The unclassified strains demonstrated variable growth with 6.5 % (w/v) NaCl and tolerated pH 6.5-9.5.
32744986	0	26	theme	Fundicoccus	0:10	arg1	nov.					25:28	Fundicoccus ignavus gen. nov.	0:28	Fundicoccus ignavus gen. nov.	0:28	Fundicoccus ignavus gen. nov., sp.
32744986	4	27	theme	tolerated	671:679	arg1	6.5-9.5					684:690	tolerated pH 6.5-9.5	671:690	tolerated pH 6.5-9.5	671:690	The unclassified strains demonstrated variable growth with 6.5 % (w/v) NaCl and tolerated pH 6.5-9.5.
32744986	4	28	theme	variable	629:636	arg1	growth					638:643	variable growth	629:643	variable growth	629:643	The unclassified strains demonstrated variable growth with 6.5 % (w/v) NaCl and tolerated pH 6.5-9.5.
32744986	12	29	theme	109652T=LMG	1456:1466	arg1	31441T					1468:1473	=DSM 109652T=LMG 31441T	1451:1473	=DSM 109652T=LMG 31441T	1451:1473	WS4937T (=DSM 109652T=LMG 31441T).
32744986	12	29	theme	109652T=LMG	1456:1466	arg1	WS4937T					1442:1448	WS4937T	1442:1448	WS4937T (=DSM 109652T=LMG 31441T).	1442:1475	WS4937T (=DSM 109652T=LMG 31441T).
32744986	7	30	theme	profile	943:949	arg1	phosphatidylglycerol					986:1005	phosphatidylglycerol	986:1005	phosphatidylglycerol	986:1005	The predominant fatty acids were C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c and in the polar lipids profile three glycolipids, a phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol were found.
32744986	7	30	theme	profile	943:949	arg1	glycolipids					957:967	the polar lipids profile three glycolipids	926:967	the polar lipids profile three glycolipids	926:967	The predominant fatty acids were C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c and in the polar lipids profile three glycolipids, a phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol were found.
32744986	7	30	theme	profile	943:949	arg1	phospholipid					972:983	a phospholipid	970:983	a phospholipid	970:983	The predominant fatty acids were C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c and in the polar lipids profile three glycolipids, a phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol were found.
32744986	7	30	theme	profile	943:949	arg1	diphosphatidylglycerol					1030:1051	diphosphatidylglycerol	1030:1051	diphosphatidylglycerol	1030:1051	The predominant fatty acids were C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c and in the polar lipids profile three glycolipids, a phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol were found.
32744986	7	30	theme	profile	943:949	arg1	phosphoglycolipid					1008:1024	phosphoglycolipid	1008:1024	phosphoglycolipid	1008:1024	The predominant fatty acids were C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c and in the polar lipids profile three glycolipids, a phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol were found.
32744986	1	31	theme	family	62:67	arg1	Aerococcaceae					69:81	the family Aerococcaceae	58:81	the family Aerococcaceae isolated from bulk tank milk	58:110	nov., a novel genus of the family Aerococcaceae isolated from bulk tank milk.
32744986	0	32	theme	gen.	20:23	arg1	nov.					25:28	Fundicoccus ignavus gen. nov.	0:28	Fundicoccus ignavus gen. nov.	0:28	Fundicoccus ignavus gen. nov., sp.
32744986	6	33	theme	A1α	776:778	arg1	l-Lys-direct					786:797	l-Lys-direct	786:797	l-Lys-direct	786:797	Their cell-wall peptidoglycan belongs to the A1α type (l-Lys-direct) consisting of alanine, glutamic acid and lysine.
32744986	6	33	theme	A1α	776:778	arg1	type					780:783	the A1α type	772:783	the A1α type (l-Lys-direct) consisting of alanine, glutamic acid and lysine	772:846	Their cell-wall peptidoglycan belongs to the A1α type (l-Lys-direct) consisting of alanine, glutamic acid and lysine.
32744986	2	34	theme	tank	246:249	arg1	milk					251:254	German bulk tank milk	234:254	German bulk tank milk	234:254	Three strains of a Gram-stain-positive, catalase-negative, facultative anaerobic, and coccoid species were isolated from German bulk tank milk.
32744986	1	35	theme	Aerococcaceae	69:81	arg1	genus					49:53	a novel genus	41:53	a novel genus of the family Aerococcaceae isolated from bulk tank milk	41:110	nov., a novel genus of the family Aerococcaceae isolated from bulk tank milk.
32744986	1	35	theme	Aerococcaceae	69:81	arg1	nov.					35:38	nov.	35:38	nov.	35:38	nov., a novel genus of the family Aerococcaceae isolated from bulk tank milk.
32744986	7	36	theme	polar	930:934	arg1	phosphatidylglycerol					986:1005	phosphatidylglycerol	986:1005	phosphatidylglycerol	986:1005	The predominant fatty acids were C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c and in the polar lipids profile three glycolipids, a phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol were found.
32744986	7	36	theme	polar	930:934	arg1	glycolipids					957:967	the polar lipids profile three glycolipids	926:967	the polar lipids profile three glycolipids	926:967	The predominant fatty acids were C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c and in the polar lipids profile three glycolipids, a phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol were found.
32744986	7	36	theme	polar	930:934	arg1	phospholipid					972:983	a phospholipid	970:983	a phospholipid	970:983	The predominant fatty acids were C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c and in the polar lipids profile three glycolipids, a phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol were found.
32744986	7	36	theme	polar	930:934	arg1	diphosphatidylglycerol					1030:1051	diphosphatidylglycerol	1030:1051	diphosphatidylglycerol	1030:1051	The predominant fatty acids were C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c and in the polar lipids profile three glycolipids, a phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol were found.
32744986	7	36	theme	polar	930:934	arg1	phosphoglycolipid					1008:1024	phosphoglycolipid	1008:1024	phosphoglycolipid	1008:1024	The predominant fatty acids were C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c and in the polar lipids profile three glycolipids, a phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol were found.
32744986	2	37	theme	Gram-stain-positive	132:150	arg1	anaerobic					184:192	a Gram-stain-positive, catalase-negative, facultative anaerobic	130:192	a Gram-stain-positive, catalase-negative, facultative anaerobic	130:192	Three strains of a Gram-stain-positive, catalase-negative, facultative anaerobic, and coccoid species were isolated from German bulk tank milk.
32744986	9	38	theme	gen.	1340:1343	arg1	nov.					1345:1348	the novel genus Fundicoccus gen. nov.	1312:1348	the novel genus Fundicoccus gen. nov.	1312:1348	Based on phylogenetic, phylogenomic and biochemical characterizations, the isolates can be demarcated from all other genera of the family Aerococcaceae and, therefore, the novel genus Fundicoccus gen. nov. is proposed.
32744986	3	39	theme	phylogenetic	404:415	arg1	lineage					417:423	an independent phylogenetic lineage	389:423	an independent phylogenetic lineage within the family Aerococcaceae with Facklamia hominis CCUG 36813T (93.7-94.1 %) and Eremococcus coleocola M1831/95/2T (93.5 %) as most closely related type species	389:588	Phylogenetic analyses based on the 16S rRNA gene sequences indicated that the three strains (WS4937T, WS4759 and WS5303) constitute an independent phylogenetic lineage within the family Aerococcaceae with Facklamia hominis CCUG 36813T (93.7-94.1 %) and Eremococcus coleocola M1831/95/2T (93.5 %) as most closely related type species.
32744986	8	40	theme	37.4 mol	1103:1110	arg1	%					1111:1111	37.4 mol%	1103:1111	37.4 mol%	1103:1111	The G+C content of strain WS4937T was 37.4 mol% with a genome size of ~3.0 Mb.
32744986	8	40	theme	37.4 mol	1103:1110	arg1	content					1073:1079	The G+C content	1065:1079	The G+C content of strain WS4937T	1065:1097	The G+C content of strain WS4937T was 37.4 mol% with a genome size of ~3.0 Mb.
32744986	7	41	theme	lipids	936:941	arg1	phosphatidylglycerol					986:1005	phosphatidylglycerol	986:1005	phosphatidylglycerol	986:1005	The predominant fatty acids were C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c and in the polar lipids profile three glycolipids, a phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol were found.
32744986	7	41	theme	lipids	936:941	arg1	glycolipids					957:967	the polar lipids profile three glycolipids	926:967	the polar lipids profile three glycolipids	926:967	The predominant fatty acids were C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c and in the polar lipids profile three glycolipids, a phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol were found.
32744986	7	41	theme	lipids	936:941	arg1	phospholipid					972:983	a phospholipid	970:983	a phospholipid	970:983	The predominant fatty acids were C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c and in the polar lipids profile three glycolipids, a phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol were found.
32744986	7	41	theme	lipids	936:941	arg1	diphosphatidylglycerol					1030:1051	diphosphatidylglycerol	1030:1051	diphosphatidylglycerol	1030:1051	The predominant fatty acids were C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c and in the polar lipids profile three glycolipids, a phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol were found.
32744986	7	41	theme	lipids	936:941	arg1	phosphoglycolipid					1008:1024	phosphoglycolipid	1008:1024	phosphoglycolipid	1008:1024	The predominant fatty acids were C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c and in the polar lipids profile three glycolipids, a phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol were found.
32744986	2	42	theme	coccoid	199:205	arg1	species					207:213	coccoid species	199:213	coccoid species	199:213	Three strains of a Gram-stain-positive, catalase-negative, facultative anaerobic, and coccoid species were isolated from German bulk tank milk.
32744986	9	43	theme	genus	1322:1326	arg1	nov.					1345:1348	the novel genus Fundicoccus gen. nov.	1312:1348	the novel genus Fundicoccus gen. nov.	1312:1348	Based on phylogenetic, phylogenomic and biochemical characterizations, the isolates can be demarcated from all other genera of the family Aerococcaceae and, therefore, the novel genus Fundicoccus gen. nov. is proposed.
32744986	10	44	theme	gen.	1422:1425	arg1	species					1372:1378	The type species	1363:1378	The type species of the novel genus	1363:1397	The type species of the novel genus is Fundicoccus ignavus gen. nov., sp.
32744986	10	44	theme	gen.	1422:1425	arg1	sp					1433:1434	sp	1433:1434	sp	1433:1434	The type species of the novel genus is Fundicoccus ignavus gen. nov., sp.
32744986	10	44	theme	gen.	1422:1425	arg1	nov.					1427:1430	Fundicoccus ignavus gen. nov.	1402:1430	Fundicoccus ignavus gen. nov.	1402:1430	The type species of the novel genus is Fundicoccus ignavus gen. nov., sp.
32744986	0	45	dep	sp	31:32	arg1	nov.					25:28	Fundicoccus ignavus gen. nov.	0:28	Fundicoccus ignavus gen. nov.	0:28	Fundicoccus ignavus gen. nov., sp.
32744986	10	46	theme	novel	1387:1391	arg1	genus					1393:1397	the novel genus	1383:1397	the novel genus	1383:1397	The type species of the novel genus is Fundicoccus ignavus gen. nov., sp.
32744986	9	47	theme	biochemical	1184:1194	arg1	characterizations					1196:1212	phylogenetic, phylogenomic and biochemical characterizations	1153:1212	phylogenetic, phylogenomic and biochemical characterizations	1153:1212	Based on phylogenetic, phylogenomic and biochemical characterizations, the isolates can be demarcated from all other genera of the family Aerococcaceae and, therefore, the novel genus Fundicoccus gen. nov. is proposed.
32744986	12	48	theme	=DSM	1451:1454	arg1	31441T					1468:1473	=DSM 109652T=LMG 31441T	1451:1473	=DSM 109652T=LMG 31441T	1451:1473	WS4937T (=DSM 109652T=LMG 31441T).
32744986	12	48	theme	=DSM	1451:1454	arg1	WS4937T					1442:1448	WS4937T	1442:1448	WS4937T (=DSM 109652T=LMG 31441T).	1442:1475	WS4937T (=DSM 109652T=LMG 31441T).
32744986	10	49	theme	genus	1393:1397	arg1	species					1372:1378	The type species	1363:1378	The type species of the novel genus	1363:1397	The type species of the novel genus is Fundicoccus ignavus gen. nov., sp.
32744986	10	49	theme	genus	1393:1397	arg1	nov.					1427:1430	Fundicoccus ignavus gen. nov.	1402:1430	Fundicoccus ignavus gen. nov.	1402:1430	The type species of the novel genus is Fundicoccus ignavus gen. nov., sp.
32744986	2	50	theme	anaerobic	184:192	arg1	species					207:213	coccoid species	199:213	coccoid species	199:213	Three strains of a Gram-stain-positive, catalase-negative, facultative anaerobic, and coccoid species were isolated from German bulk tank milk.
32744986	2	50	theme	anaerobic	184:192	arg1	strains					119:125	Three strains	113:125	Three strains of a Gram-stain-positive, catalase-negative, facultative anaerobic	113:192	Three strains of a Gram-stain-positive, catalase-negative, facultative anaerobic, and coccoid species were isolated from German bulk tank milk.
32744986	9	51	theme	phylogenomic	1167:1178	arg1	characterizations					1196:1212	phylogenetic, phylogenomic and biochemical characterizations	1153:1212	phylogenetic, phylogenomic and biochemical characterizations	1153:1212	Based on phylogenetic, phylogenomic and biochemical characterizations, the isolates can be demarcated from all other genera of the family Aerococcaceae and, therefore, the novel genus Fundicoccus gen. nov. is proposed.
32744986	3	52	theme	Facklamia	462:470	arg1	36813T					485:490	Facklamia hominis CCUG 36813T	462:490	Facklamia hominis CCUG 36813T (93.7-94.1 %)	462:504	Phylogenetic analyses based on the 16S rRNA gene sequences indicated that the three strains (WS4937T, WS4759 and WS5303) constitute an independent phylogenetic lineage within the family Aerococcaceae with Facklamia hominis CCUG 36813T (93.7-94.1 %) and Eremococcus coleocola M1831/95/2T (93.5 %) as most closely related type species.
32744986	3	52	theme	Facklamia	462:470	arg1	%					503:503	93.7-94.1 %	493:503	93.7-94.1 %	493:503	Phylogenetic analyses based on the 16S rRNA gene sequences indicated that the three strains (WS4937T, WS4759 and WS5303) constitute an independent phylogenetic lineage within the family Aerococcaceae with Facklamia hominis CCUG 36813T (93.7-94.1 %) and Eremococcus coleocola M1831/95/2T (93.5 %) as most closely related type species.
32744986	3	53	theme	Phylogenetic	257:268	arg1	analyses					270:277	Phylogenetic analyses	257:277	Phylogenetic analyses based on the 16S rRNA gene sequences	257:314	Phylogenetic analyses based on the 16S rRNA gene sequences indicated that the three strains (WS4937T, WS4759 and WS5303) constitute an independent phylogenetic lineage within the family Aerococcaceae with Facklamia hominis CCUG 36813T (93.7-94.1 %) and Eremococcus coleocola M1831/95/2T (93.5 %) as most closely related type species.
32744986	3	54	theme	related	569:575	arg1	species					582:588	most closely related type species	556:588	most closely related type species	556:588	Phylogenetic analyses based on the 16S rRNA gene sequences indicated that the three strains (WS4937T, WS4759 and WS5303) constitute an independent phylogenetic lineage within the family Aerococcaceae with Facklamia hominis CCUG 36813T (93.7-94.1 %) and Eremococcus coleocola M1831/95/2T (93.5 %) as most closely related type species.
32744986	2	55	theme	German	234:239	arg1	milk					251:254	German bulk tank milk	234:254	German bulk tank milk	234:254	Three strains of a Gram-stain-positive, catalase-negative, facultative anaerobic, and coccoid species were isolated from German bulk tank milk.
32744986	1	56	theme	bulk	97:100	arg1	milk					107:110	bulk tank milk	97:110	bulk tank milk	97:110	nov., a novel genus of the family Aerococcaceae isolated from bulk tank milk.
32744986	7	57	from	 1 ω9c	912:917	arg1	phosphatidylglycerol					986:1005	phosphatidylglycerol	986:1005	phosphatidylglycerol	986:1005	The predominant fatty acids were C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c and in the polar lipids profile three glycolipids, a phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol were found.
32744986	7	57	from	 1 ω9c	912:917	arg1	glycolipids					957:967	the polar lipids profile three glycolipids	926:967	the polar lipids profile three glycolipids	926:967	The predominant fatty acids were C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c and in the polar lipids profile three glycolipids, a phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol were found.
32744986	7	57	from	 1 ω9c	912:917	arg1	phospholipid					972:983	a phospholipid	970:983	a phospholipid	970:983	The predominant fatty acids were C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c and in the polar lipids profile three glycolipids, a phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol were found.
32744986	7	57	from	 1 ω9c	912:917	arg1	diphosphatidylglycerol					1030:1051	diphosphatidylglycerol	1030:1051	diphosphatidylglycerol	1030:1051	The predominant fatty acids were C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c and in the polar lipids profile three glycolipids, a phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol were found.
32744986	7	57	from	 1 ω9c	912:917	arg1	phosphoglycolipid					1008:1024	phosphoglycolipid	1008:1024	phosphoglycolipid	1008:1024	The predominant fatty acids were C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c and in the polar lipids profile three glycolipids, a phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol were found.
32744986	3	58	dep	Facklamia	462:470	arg1	hominis					472:478	hominis	472:478	hominis	472:478	Phylogenetic analyses based on the 16S rRNA gene sequences indicated that the three strains (WS4937T, WS4759 and WS5303) constitute an independent phylogenetic lineage within the family Aerococcaceae with Facklamia hominis CCUG 36813T (93.7-94.1 %) and Eremococcus coleocola M1831/95/2T (93.5 %) as most closely related type species.
32744986	3	59	theme	type	577:580	arg1	species					582:588	most closely related type species	556:588	most closely related type species	556:588	Phylogenetic analyses based on the 16S rRNA gene sequences indicated that the three strains (WS4937T, WS4759 and WS5303) constitute an independent phylogenetic lineage within the family Aerococcaceae with Facklamia hominis CCUG 36813T (93.7-94.1 %) and Eremococcus coleocola M1831/95/2T (93.5 %) as most closely related type species.
32744986	1	60	theme	tank	102:105	arg1	milk					107:110	bulk tank milk	97:110	bulk tank milk	97:110	nov., a novel genus of the family Aerococcaceae isolated from bulk tank milk.
32744986	7	61	dep	C16 	882:885	arg1	C16 					895:898	C16 	895:898	C16 	895:898	The predominant fatty acids were C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c and in the polar lipids profile three glycolipids, a phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol were found.
32744986	7	61	dep	C16 	882:885	arg1	C18 					907:910	C18 	907:910	C18 	907:910	The predominant fatty acids were C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c and in the polar lipids profile three glycolipids, a phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol were found.
32744986	7	61	dep	C16 	882:885	arg1	 0					900:901	 0	900:901	 0	900:901	The predominant fatty acids were C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c and in the polar lipids profile three glycolipids, a phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol were found.
32744986	3	62	theme	family	436:441	arg1	Aerococcaceae					443:455	the family Aerococcaceae	432:455	the family Aerococcaceae with Facklamia hominis CCUG 36813T (93.7-94.1 %) and Eremococcus coleocola M1831/95/2T (93.5 %) as most closely related type species	432:588	Phylogenetic analyses based on the 16S rRNA gene sequences indicated that the three strains (WS4937T, WS4759 and WS5303) constitute an independent phylogenetic lineage within the family Aerococcaceae with Facklamia hominis CCUG 36813T (93.7-94.1 %) and Eremococcus coleocola M1831/95/2T (93.5 %) as most closely related type species.
32744986	3	63	theme	rRNA	296:299	arg1	sequences					306:314	the 16S rRNA gene sequences	288:314	the 16S rRNA gene sequences	288:314	Phylogenetic analyses based on the 16S rRNA gene sequences indicated that the three strains (WS4937T, WS4759 and WS5303) constitute an independent phylogenetic lineage within the family Aerococcaceae with Facklamia hominis CCUG 36813T (93.7-94.1 %) and Eremococcus coleocola M1831/95/2T (93.5 %) as most closely related type species.
32744986	9	64	theme	phylogenetic	1153:1164	arg1	characterizations					1196:1212	phylogenetic, phylogenomic and biochemical characterizations	1153:1212	phylogenetic, phylogenomic and biochemical characterizations	1153:1212	Based on phylogenetic, phylogenomic and biochemical characterizations, the isolates can be demarcated from all other genera of the family Aerococcaceae and, therefore, the novel genus Fundicoccus gen. nov. is proposed.
32744986	4	65	theme	6.5 	650:653	arg1	%					654:654	%	654:654	%	654:654	The unclassified strains demonstrated variable growth with 6.5 % (w/v) NaCl and tolerated pH 6.5-9.5.
32744986	8	66	theme	genome	1120:1125	arg1	size					1127:1130	a genome size	1118:1130	a genome size of ~3.0 Mb	1118:1141	The G+C content of strain WS4937T was 37.4 mol% with a genome size of ~3.0 Mb.
32744986	9	67	theme	Aerococcaceae	1282:1294	arg1	genera					1261:1266	all other genera	1251:1266	all other genera of the family Aerococcaceae	1251:1294	Based on phylogenetic, phylogenomic and biochemical characterizations, the isolates can be demarcated from all other genera of the family Aerococcaceae and, therefore, the novel genus Fundicoccus gen. nov. is proposed.
32744986	3	68	theme	gene	301:304	arg1	sequences					306:314	the 16S rRNA gene sequences	288:314	the 16S rRNA gene sequences	288:314	Phylogenetic analyses based on the 16S rRNA gene sequences indicated that the three strains (WS4937T, WS4759 and WS5303) constitute an independent phylogenetic lineage within the family Aerococcaceae with Facklamia hominis CCUG 36813T (93.7-94.1 %) and Eremococcus coleocola M1831/95/2T (93.5 %) as most closely related type species.
32744986	9	69	theme	Fundicoccus	1328:1338	arg1	nov.					1345:1348	the novel genus Fundicoccus gen. nov.	1312:1348	the novel genus Fundicoccus gen. nov.	1312:1348	Based on phylogenetic, phylogenomic and biochemical characterizations, the isolates can be demarcated from all other genera of the family Aerococcaceae and, therefore, the novel genus Fundicoccus gen. nov. is proposed.
32744986	8	70	theme	WS4937T	1091:1097	arg1	%					1111:1111	37.4 mol%	1103:1111	37.4 mol%	1103:1111	The G+C content of strain WS4937T was 37.4 mol% with a genome size of ~3.0 Mb.
32744986	8	70	theme	WS4937T	1091:1097	arg1	content					1073:1079	The G+C content	1065:1079	The G+C content of strain WS4937T	1065:1097	The G+C content of strain WS4937T was 37.4 mol% with a genome size of ~3.0 Mb.
32744986	8	71	with	%	1111:1111	arg1	size					1127:1130	a genome size	1118:1130	a genome size of ~3.0 Mb	1118:1141	The G+C content of strain WS4937T was 37.4 mol% with a genome size of ~3.0 Mb.
32744986	8	72	theme	strain	1084:1089	arg1	WS4937T					1091:1097	strain WS4937T	1084:1097	strain WS4937T	1084:1097	The G+C content of strain WS4937T was 37.4 mol% with a genome size of ~3.0 Mb.
32744986	2	73	attach	isolated	220:227	arg2	strains					119:125	Three strains	113:125	Three strains of a Gram-stain-positive, catalase-negative, facultative anaerobic	113:192	Three strains of a Gram-stain-positive, catalase-negative, facultative anaerobic, and coccoid species were isolated from German bulk tank milk.
32744986	2	73	attach	isolated	220:227	arg1	milk					251:254	German bulk tank milk	234:254	German bulk tank milk	234:254	Three strains of a Gram-stain-positive, catalase-negative, facultative anaerobic, and coccoid species were isolated from German bulk tank milk.
32744986	2	73	attach	isolated	220:227	arg2	species					207:213	coccoid species	199:213	coccoid species	199:213	Three strains of a Gram-stain-positive, catalase-negative, facultative anaerobic, and coccoid species were isolated from German bulk tank milk.
32253039	11	0	theme	repeated	1813:1820	arg1	subject					1822:1828	repeated subject	1813:1828	repeated subject	1813:1828	Data were evaluated using mixed models with time, group, and time × group interaction as fixed effects and cow as repeated subject.
32253039	6	1	theme	60-min	985:990	arg1	intervals					992:1000	30- and 60-min intervals	977:1000	30- and 60-min intervals	977:1000	Blood and milk samples were taken in parallel at 30- and 60-min intervals, respectively, until 10 h after the LPS application.
32253039	16	2	theme	serum	2454:2458	arg1	albumin					2460:2466	serum albumin	2454:2466	serum albumin	2454:2466	During 8 d after LPS-CH, SCC, LDH, IgG, and serum albumin in milk were lower in HG compared with LG.
32253039	8	3	theme	cell	1229:1232	arg1	SCC					1241:1243	SCC	1241:1243	SCC	1241:1243	In milk, serum albumin, IgG concentration, somatic cell count (SCC), and lactate dehydrogenase (LDH) activity were determined.
32253039	8	3	theme	cell	1229:1232	arg1	count					1234:1238	somatic cell count	1221:1238	somatic cell count (SCC)	1221:1244	In milk, serum albumin, IgG concentration, somatic cell count (SCC), and lactate dehydrogenase (LDH) activity were determined.
32253039	17	4	theme	metabolic	2610:2618	arg1	responses					2620:2628	metabolic responses	2610:2628	metabolic responses during an LPS-CH as well as the recovery of udder health and performance thereafter	2610:2712	In conclusion, the level of circulating glucose and BHB concentrations in cows was associated with metabolic responses during an LPS-CH as well as the recovery of udder health and performance thereafter.
32253039	15	5	with	LPS-CH	2311:2316	arg1	differences					2326:2336	no differences	2323:2336	no differences in morning milkings	2323:2356	Milk yield recovered within 2 d after the LPS-CH with no differences in morning milkings, whereas evening milk yield increased faster in HG.
32253039	15	6	theme	evening	2367:2373	arg1	yield					2380:2384	evening milk yield	2367:2384	evening milk yield	2367:2384	Milk yield recovered within 2 d after the LPS-CH with no differences in morning milkings, whereas evening milk yield increased faster in HG.
32253039	15	7	theme	morning	2341:2347	arg1	milkings					2349:2356	morning milkings	2341:2356	morning milkings	2341:2356	Milk yield recovered within 2 d after the LPS-CH with no differences in morning milkings, whereas evening milk yield increased faster in HG.
32253039	1	8	theme	mammary	170:176	arg1	gland					178:182	the mammary gland	166:182	the mammary gland in dairy cows	166:196	Infections of the mammary gland in dairy cows are commonly accompanied by reduced milk production and feed intake and poor milk quality.
32253039	6	9	theme	LPS	1038:1040	arg1	application					1042:1052	the LPS application	1034:1052	the LPS application	1034:1052	Blood and milk samples were taken in parallel at 30- and 60-min intervals, respectively, until 10 h after the LPS application.
32253039	2	10	theme	metabolic	293:301	arg1	status					303:308	The metabolic status	289:308	The metabolic status of early-lactating cows	289:332	The metabolic status of early-lactating cows is known to affect immune response to pathogens and imposed immune challenges.
32253039	11	11	theme	group	1767:1771	arg1	interaction					1773:1783	time × group interaction	1760:1783	time × group interaction	1760:1783	Data were evaluated using mixed models with time, group, and time × group interaction as fixed effects and cow as repeated subject.
32253039	8	12	theme	somatic	1221:1227	arg1	SCC					1241:1243	SCC	1241:1243	SCC	1241:1243	In milk, serum albumin, IgG concentration, somatic cell count (SCC), and lactate dehydrogenase (LDH) activity were determined.
32253039	8	12	theme	somatic	1221:1227	arg1	count					1234:1238	somatic cell count	1221:1238	somatic cell count (SCC)	1221:1244	In milk, serum albumin, IgG concentration, somatic cell count (SCC), and lactate dehydrogenase (LDH) activity were determined.
32253039	0	13	theme	lipopolysaccharide-induced	101:126	arg1	mastitis					128:135	lipopolysaccharide-induced mastitis	101:135	lipopolysaccharide-induced mastitis in dairy cows	101:149	Metabolic status is associated with the recovery of milk somatic cell count and milk secretion after lipopolysaccharide-induced mastitis in dairy cows.
32253039	9	14	theme	Dry	1305:1307	arg1	intake					1316:1321	Dry matter intake	1305:1321	Dry matter intake	1305:1321	Dry matter intake and milk yield were recorded for an additional 6 d. Milk of the LPS-treated quarter was sampled at every milking for 8 d after the challenge.
32253039	14	15	theme	prechallenge	2235:2246	arg1	values					2248:2253	prechallenge values	2235:2253	prechallenge values	2235:2253	Dry matter intake declined in both groups during the day of the LPS-CH but recovered to prechallenge values faster in HG.
32253039	11	16	theme	time	1760:1763	arg1	interaction					1773:1783	time × group interaction	1760:1783	time × group interaction	1760:1783	Data were evaluated using mixed models with time, group, and time × group interaction as fixed effects and cow as repeated subject.
32253039	4	17	theme	daily	675:679	arg1	recording					681:689	daily recording	675:689	daily recording	675:689	In 15 Holstein cows, weekly blood sampling and daily recording of dry matter intake, milk yield, milk composition, and body weight (to calculate energy balance) was started immediately after parturition.
32253039	13	18	theme	nonesterified	2085:2097	arg1	acids					2105:2109	nonesterified fatty acids	2085:2109	nonesterified fatty acids	2085:2109	During LPS-CH, SCC and LDH increased similarly in HG and LG, body temperature increased less in HG, and BHB and nonesterified fatty acids were higher in LG compared with HG.
32253039	3	19	theme	milk	569:572	arg1	production					574:583	milk production	569:583	milk production	569:583	We investigated the extent to which metabolic status before an intramammary lipopolysaccharide (LPS) challenge (LPS-CH) is associated with immune response, milk production, and feed intake and the recovery thereof.
32253039	9	20	theme	milk	1327:1330	arg1	yield					1332:1336	milk yield	1327:1336	milk yield	1327:1336	Dry matter intake and milk yield were recorded for an additional 6 d. Milk of the LPS-treated quarter was sampled at every milking for 8 d after the challenge.
32253039	14	21	theme	LPS-CH	2211:2216	arg1	day					2200:2202	the day	2196:2202	the day of the LPS-CH	2196:2216	Dry matter intake declined in both groups during the day of the LPS-CH but recovered to prechallenge values faster in HG.
32253039	9	22	theme	quarter	1399:1405	arg1	Milk					1375:1378	an additional 6 d. Milk	1356:1378	an additional 6 d. Milk of the LPS-treated quarter was sampled at every milking for 8 d after the challenge	1356:1462	Dry matter intake and milk yield were recorded for an additional 6 d. Milk of the LPS-treated quarter was sampled at every milking for 8 d after the challenge.
32253039	17	23	theme	health	2680:2685	arg1	recovery					2662:2669	the recovery	2658:2669	the recovery of udder health and performance thereafter	2658:2712	In conclusion, the level of circulating glucose and BHB concentrations in cows was associated with metabolic responses during an LPS-CH as well as the recovery of udder health and performance thereafter.
32253039	17	23	theme	health	2680:2685	arg1	LPS-CH					2640:2645	an LPS-CH	2637:2645	an LPS-CH	2637:2645	In conclusion, the level of circulating glucose and BHB concentrations in cows was associated with metabolic responses during an LPS-CH as well as the recovery of udder health and performance thereafter.
32253039	1	24	from	gland	178:182	arg1	cows					193:196	dairy cows	187:196	dairy cows	187:196	Infections of the mammary gland in dairy cows are commonly accompanied by reduced milk production and feed intake and poor milk quality.
32253039	6	25	theme	Blood	928:932	arg1	samples					943:949	Blood and milk samples	928:949	Blood and milk samples	928:949	Blood and milk samples were taken in parallel at 30- and 60-min intervals, respectively, until 10 h after the LPS application.
32253039	4	26	theme	weekly	649:654	arg1	sampling					662:669	weekly blood sampling	649:669	weekly blood sampling	649:669	In 15 Holstein cows, weekly blood sampling and daily recording of dry matter intake, milk yield, milk composition, and body weight (to calculate energy balance) was started immediately after parturition.
32253039	17	27	theme	performance	2691:2701	arg1	recovery					2662:2669	the recovery	2658:2669	the recovery of udder health and performance thereafter	2658:2712	In conclusion, the level of circulating glucose and BHB concentrations in cows was associated with metabolic responses during an LPS-CH as well as the recovery of udder health and performance thereafter.
32253039	17	27	theme	performance	2691:2701	arg1	LPS-CH					2640:2645	an LPS-CH	2637:2645	an LPS-CH	2637:2645	In conclusion, the level of circulating glucose and BHB concentrations in cows was associated with metabolic responses during an LPS-CH as well as the recovery of udder health and performance thereafter.
32253039	7	28	theme	β-hydroxybutyrate	1116:1132	arg1	concentrations					1062:1075	Plasma concentrations	1055:1075	Plasma concentrations of glucose, nonesterified fatty acids, β-hydroxybutyrate (BHB), cortisol, and insulin	1055:1161	Plasma concentrations of glucose, nonesterified fatty acids, β-hydroxybutyrate (BHB), cortisol, and insulin were analyzed.
32253039	3	29	theme	feed	590:593	arg1	intake					595:600	feed intake	590:600	feed intake	590:600	We investigated the extent to which metabolic status before an intramammary lipopolysaccharide (LPS) challenge (LPS-CH) is associated with immune response, milk production, and feed intake and the recovery thereof.
32253039	0	30	theme	cell	65:68	arg1	count					70:74	milk somatic cell count	52:74	milk somatic cell count	52:74	Metabolic status is associated with the recovery of milk somatic cell count and milk secretion after lipopolysaccharide-induced mastitis in dairy cows.
32253039	1	31	from	Infections	152:161	arg1	cows					193:196	dairy cows	187:196	dairy cows	187:196	Infections of the mammary gland in dairy cows are commonly accompanied by reduced milk production and feed intake and poor milk quality.
32253039	9	32	theme	additional	1359:1368	arg1	Milk					1375:1378	an additional 6 d. Milk	1356:1378	an additional 6 d. Milk of the LPS-treated quarter was sampled at every milking for 8 d after the challenge	1356:1462	Dry matter intake and milk yield were recorded for an additional 6 d. Milk of the LPS-treated quarter was sampled at every milking for 8 d after the challenge.
32253039	8	33	theme	IgG	1202:1204	arg1	concentration					1206:1218	IgG concentration	1202:1218	IgG concentration	1202:1218	In milk, serum albumin, IgG concentration, somatic cell count (SCC), and lactate dehydrogenase (LDH) activity were determined.
32253039	7	34	theme	nonesterified	1089:1101	arg1	acids					1109:1113	nonesterified fatty acids	1089:1113	nonesterified fatty acids	1089:1113	Plasma concentrations of glucose, nonesterified fatty acids, β-hydroxybutyrate (BHB), cortisol, and insulin were analyzed.
32253039	9	35	theme	d.	1372:1373	arg1	Milk					1375:1378	an additional 6 d. Milk	1356:1378	an additional 6 d. Milk of the LPS-treated quarter was sampled at every milking for 8 d after the challenge	1356:1462	Dry matter intake and milk yield were recorded for an additional 6 d. Milk of the LPS-treated quarter was sampled at every milking for 8 d after the challenge.
32253039	15	36	from	differences	2326:2336	arg1	milkings					2349:2356	morning milkings	2341:2356	morning milkings	2341:2356	Milk yield recovered within 2 d after the LPS-CH with no differences in morning milkings, whereas evening milk yield increased faster in HG.
32253039	4	37	theme	milk	725:728	arg1	composition					730:740	milk composition	725:740	milk composition	725:740	In 15 Holstein cows, weekly blood sampling and daily recording of dry matter intake, milk yield, milk composition, and body weight (to calculate energy balance) was started immediately after parturition.
32253039	0	38	theme	secretion	85:93	arg1	recovery					40:47	the recovery	36:47	the recovery of milk somatic cell count and milk secretion after lipopolysaccharide-induced mastitis in dairy cows	36:149	Metabolic status is associated with the recovery of milk somatic cell count and milk secretion after lipopolysaccharide-induced mastitis in dairy cows.
32253039	4	39	theme	matter	698:703	arg1	intake					705:710	dry matter intake	694:710	dry matter intake	694:710	In 15 Holstein cows, weekly blood sampling and daily recording of dry matter intake, milk yield, milk composition, and body weight (to calculate energy balance) was started immediately after parturition.
32253039	0	40	theme	Metabolic	0:8	arg1	status					10:15	Metabolic status	0:15	Metabolic status	0:15	Metabolic status is associated with the recovery of milk somatic cell count and milk secretion after lipopolysaccharide-induced mastitis in dairy cows.
32253039	9	41	theme	LPS-treated	1387:1397	arg1	quarter					1399:1405	the LPS-treated quarter	1383:1405	the LPS-treated quarter was sampled at every milking for 8 d after the challenge	1383:1462	Dry matter intake and milk yield were recorded for an additional 6 d. Milk of the LPS-treated quarter was sampled at every milking for 8 d after the challenge.
32253039	3	42	theme	metabolic	449:457	arg1	status					459:464	metabolic status	449:464	metabolic status before an intramammary lipopolysaccharide (LPS) challenge (LPS-CH)	449:531	We investigated the extent to which metabolic status before an intramammary lipopolysaccharide (LPS) challenge (LPS-CH) is associated with immune response, milk production, and feed intake and the recovery thereof.
32253039	1	43	theme	milk	275:278	arg1	quality					280:286	poor milk quality	270:286	poor milk quality	270:286	Infections of the mammary gland in dairy cows are commonly accompanied by reduced milk production and feed intake and poor milk quality.
32253039	10	44	theme	=	1639:1639	arg1	n					1637:1637	n = 7	1637:1641	n = 7	1637:1641	Based on plasma glucose concentrations in wk 1 to 4 after parturition before the LPS-CH, cows were retrospectively grouped into a high-glucose group (HG; 3.34-3.93 mmol/L, n = 7) and a low-glucose group (LG; 2.87-3.31 mmol/L, n = 8).
32253039	10	45	theme	plasma	1474:1479	arg1	concentrations					1489:1502	plasma glucose concentrations	1474:1502	plasma glucose concentrations in wk 1 to 4 after parturition before the LPS-CH	1474:1551	Based on plasma glucose concentrations in wk 1 to 4 after parturition before the LPS-CH, cows were retrospectively grouped into a high-glucose group (HG; 3.34-3.93 mmol/L, n = 7) and a low-glucose group (LG; 2.87-3.31 mmol/L, n = 8).
32253039	11	46	with	models	1731:1736	arg1	group					1749:1753	group	1749:1753	group	1749:1753	Data were evaluated using mixed models with time, group, and time × group interaction as fixed effects and cow as repeated subject.
32253039	11	46	with	models	1731:1736	arg1	interaction					1773:1783	time × group interaction	1760:1783	time × group interaction	1760:1783	Data were evaluated using mixed models with time, group, and time × group interaction as fixed effects and cow as repeated subject.
32253039	11	46	with	models	1731:1736	arg1	time					1743:1746	time	1743:1746	time	1743:1746	Data were evaluated using mixed models with time, group, and time × group interaction as fixed effects and cow as repeated subject.
32253039	14	47	theme	matter	2151:2156	arg1	intake					2158:2163	Dry matter intake	2147:2163	Dry matter intake	2147:2163	Dry matter intake declined in both groups during the day of the LPS-CH but recovered to prechallenge values faster in HG.
32253039	6	48	theme	milk	938:941	arg1	samples					943:949	Blood and milk samples	928:949	Blood and milk samples	928:949	Blood and milk samples were taken in parallel at 30- and 60-min intervals, respectively, until 10 h after the LPS application.
32253039	0	49	theme	milk	52:55	arg1	count					70:74	milk somatic cell count	52:74	milk somatic cell count	52:74	Metabolic status is associated with the recovery of milk somatic cell count and milk secretion after lipopolysaccharide-induced mastitis in dairy cows.
32253039	3	50	theme	intramammary	476:487	arg1	LPS					509:511	LPS	509:511	LPS	509:511	We investigated the extent to which metabolic status before an intramammary lipopolysaccharide (LPS) challenge (LPS-CH) is associated with immune response, milk production, and feed intake and the recovery thereof.
32253039	3	50	theme	intramammary	476:487	arg1	lipopolysaccharide					489:506	an intramammary lipopolysaccharide	473:506	an intramammary lipopolysaccharide (LPS) challenge (LPS-CH)	473:531	We investigated the extent to which metabolic status before an intramammary lipopolysaccharide (LPS) challenge (LPS-CH) is associated with immune response, milk production, and feed intake and the recovery thereof.
32253039	13	51	theme	body	2034:2037	arg1	temperature					2039:2049	body temperature	2034:2049	body temperature	2034:2049	During LPS-CH, SCC and LDH increased similarly in HG and LG, body temperature increased less in HG, and BHB and nonesterified fatty acids were higher in LG compared with HG.
32253039	7	52	theme	insulin	1155:1161	arg1	concentrations					1062:1075	Plasma concentrations	1055:1075	Plasma concentrations of glucose, nonesterified fatty acids, β-hydroxybutyrate (BHB), cortisol, and insulin	1055:1161	Plasma concentrations of glucose, nonesterified fatty acids, β-hydroxybutyrate (BHB), cortisol, and insulin were analyzed.
32253039	10	53	dep	LG	1669:1670	arg1	mmol/L					1683:1688	2.87-3.31 mmol/L	1673:1688	2.87-3.31 mmol/L	1673:1688	Based on plasma glucose concentrations in wk 1 to 4 after parturition before the LPS-CH, cows were retrospectively grouped into a high-glucose group (HG; 3.34-3.93 mmol/L, n = 7) and a low-glucose group (LG; 2.87-3.31 mmol/L, n = 8).
32253039	10	53	dep	LG	1669:1670	arg1	n					1691:1691	n = 8	1691:1695	n = 8	1691:1695	Based on plasma glucose concentrations in wk 1 to 4 after parturition before the LPS-CH, cows were retrospectively grouped into a high-glucose group (HG; 3.34-3.93 mmol/L, n = 7) and a low-glucose group (LG; 2.87-3.31 mmol/L, n = 8).
32253039	8	54	theme	dehydrogenase	1259:1271	arg1	activity					1279:1286	lactate dehydrogenase (LDH) activity	1251:1286	lactate dehydrogenase (LDH) activity	1251:1286	In milk, serum albumin, IgG concentration, somatic cell count (SCC), and lactate dehydrogenase (LDH) activity were determined.
32253039	2	55	theme	early-lactating	313:327	arg1	cows					329:332	early-lactating cows	313:332	early-lactating cows	313:332	The metabolic status of early-lactating cows is known to affect immune response to pathogens and imposed immune challenges.
32253039	17	56	theme	glucose	2551:2557	arg1	concentrations					2567:2580	glucose and BHB concentrations	2551:2580	glucose and BHB concentrations	2551:2580	In conclusion, the level of circulating glucose and BHB concentrations in cows was associated with metabolic responses during an LPS-CH as well as the recovery of udder health and performance thereafter.
32253039	12	57	theme	dry	1914:1916	arg1	intake					1925:1930	dry matter intake	1914:1930	dry matter intake	1914:1930	Glucose was lower and BHB was higher in LG compared with HG before LPS-CH, whereas dry matter intake, energy balance, and SCC did not differ.
32253039	17	58	theme	udder	2674:2678	arg1	health					2680:2685	udder health	2674:2685	udder health	2674:2685	In conclusion, the level of circulating glucose and BHB concentrations in cows was associated with metabolic responses during an LPS-CH as well as the recovery of udder health and performance thereafter.
32253039	4	59	theme	body	747:750	arg1	weight					752:757	body weight	747:757	body weight	747:757	In 15 Holstein cows, weekly blood sampling and daily recording of dry matter intake, milk yield, milk composition, and body weight (to calculate energy balance) was started immediately after parturition.
32253039	2	60	theme	immune	353:358	arg1	response					360:367	immune response	353:367	immune response to pathogens and imposed immune challenges	353:410	The metabolic status of early-lactating cows is known to affect immune response to pathogens and imposed immune challenges.
32253039	1	61	theme	reduced	226:232	arg1	production					239:248	reduced milk production	226:248	reduced milk production	226:248	Infections of the mammary gland in dairy cows are commonly accompanied by reduced milk production and feed intake and poor milk quality.
32253039	10	62	from	concentrations	1489:1502	arg1	wk					1507:1508	wk 1 to 4	1507:1515	wk 1 to 4 after parturition before the LPS-CH	1507:1551	Based on plasma glucose concentrations in wk 1 to 4 after parturition before the LPS-CH, cows were retrospectively grouped into a high-glucose group (HG; 3.34-3.93 mmol/L, n = 7) and a low-glucose group (LG; 2.87-3.31 mmol/L, n = 8).
32253039	7	63	theme	fatty	1103:1107	arg1	acids					1109:1113	nonesterified fatty acids	1089:1113	nonesterified fatty acids	1089:1113	Plasma concentrations of glucose, nonesterified fatty acids, β-hydroxybutyrate (BHB), cortisol, and insulin were analyzed.
32253039	15	64	theme	milk	2375:2378	arg1	yield					2380:2384	evening milk yield	2367:2384	evening milk yield	2367:2384	Milk yield recovered within 2 d after the LPS-CH with no differences in morning milkings, whereas evening milk yield increased faster in HG.
32253039	1	65	theme	feed	254:257	arg1	intake					259:264	feed intake	254:264	feed intake	254:264	Infections of the mammary gland in dairy cows are commonly accompanied by reduced milk production and feed intake and poor milk quality.
32253039	11	66	theme	mixed	1725:1729	arg1	effects					1794:1800	fixed effects	1788:1800	fixed effects	1788:1800	Data were evaluated using mixed models with time, group, and time × group interaction as fixed effects and cow as repeated subject.
32253039	11	66	theme	mixed	1725:1729	arg1	models					1731:1736	mixed models	1725:1736	mixed models with time, group, and time × group interaction	1725:1783	Data were evaluated using mixed models with time, group, and time × group interaction as fixed effects and cow as repeated subject.
32253039	4	67	theme	energy	773:778	arg1	balance					780:786	energy balance	773:786	energy balance	773:786	In 15 Holstein cows, weekly blood sampling and daily recording of dry matter intake, milk yield, milk composition, and body weight (to calculate energy balance) was started immediately after parturition.
32253039	10	68	dep	HG	1615:1616	arg1	n					1637:1637	n = 7	1637:1641	n = 7	1637:1641	Based on plasma glucose concentrations in wk 1 to 4 after parturition before the LPS-CH, cows were retrospectively grouped into a high-glucose group (HG; 3.34-3.93 mmol/L, n = 7) and a low-glucose group (LG; 2.87-3.31 mmol/L, n = 8).
32253039	10	68	dep	HG	1615:1616	arg1	mmol/L					1629:1634	3.34-3.93 mmol/L	1619:1634	3.34-3.93 mmol/L	1619:1634	Based on plasma glucose concentrations in wk 1 to 4 after parturition before the LPS-CH, cows were retrospectively grouped into a high-glucose group (HG; 3.34-3.93 mmol/L, n = 7) and a low-glucose group (LG; 2.87-3.31 mmol/L, n = 8).
32253039	7	69	theme	acids	1109:1113	arg1	concentrations					1062:1075	Plasma concentrations	1055:1075	Plasma concentrations of glucose, nonesterified fatty acids, β-hydroxybutyrate (BHB), cortisol, and insulin	1055:1161	Plasma concentrations of glucose, nonesterified fatty acids, β-hydroxybutyrate (BHB), cortisol, and insulin were analyzed.
32253039	3	70	theme	immune	552:557	arg1	response					559:566	immune response	552:566	immune response	552:566	We investigated the extent to which metabolic status before an intramammary lipopolysaccharide (LPS) challenge (LPS-CH) is associated with immune response, milk production, and feed intake and the recovery thereof.
32253039	1	71	theme	gland	178:182	arg1	Infections					152:161	Infections	152:161	Infections of the mammary gland in dairy cows	152:196	Infections of the mammary gland in dairy cows are commonly accompanied by reduced milk production and feed intake and poor milk quality.
32253039	4	72	theme	Holstein	634:641	arg1	cows					643:646	15 Holstein cows	631:646	15 Holstein cows	631:646	In 15 Holstein cows, weekly blood sampling and daily recording of dry matter intake, milk yield, milk composition, and body weight (to calculate energy balance) was started immediately after parturition.
32253039	1	73	theme	dairy	187:191	arg1	cows					193:196	dairy cows	187:196	dairy cows	187:196	Infections of the mammary gland in dairy cows are commonly accompanied by reduced milk production and feed intake and poor milk quality.
32253039	17	74	theme	BHB	2563:2565	arg1	concentrations					2567:2580	glucose and BHB concentrations	2551:2580	glucose and BHB concentrations	2551:2580	In conclusion, the level of circulating glucose and BHB concentrations in cows was associated with metabolic responses during an LPS-CH as well as the recovery of udder health and performance thereafter.
32253039	16	75	from	albumin	2460:2466	arg1	milk					2471:2474	milk	2471:2474	milk	2471:2474	During 8 d after LPS-CH, SCC, LDH, IgG, and serum albumin in milk were lower in HG compared with LG.
32253039	8	76	theme	serum	1187:1191	arg1	albumin					1193:1199	serum albumin	1187:1199	serum albumin	1187:1199	In milk, serum albumin, IgG concentration, somatic cell count (SCC), and lactate dehydrogenase (LDH) activity were determined.
32253039	10	77	theme	=	1693:1693	arg1	n					1691:1691	n = 8	1691:1695	n = 8	1691:1695	Based on plasma glucose concentrations in wk 1 to 4 after parturition before the LPS-CH, cows were retrospectively grouped into a high-glucose group (HG; 3.34-3.93 mmol/L, n = 7) and a low-glucose group (LG; 2.87-3.31 mmol/L, n = 8).
32253039	0	78	theme	dairy	140:144	arg1	cows					146:149	dairy cows	140:149	dairy cows	140:149	Metabolic status is associated with the recovery of milk somatic cell count and milk secretion after lipopolysaccharide-induced mastitis in dairy cows.
32253039	9	79	theme	matter	1309:1314	arg1	intake					1316:1321	Dry matter intake	1305:1321	Dry matter intake	1305:1321	Dry matter intake and milk yield were recorded for an additional 6 d. Milk of the LPS-treated quarter was sampled at every milking for 8 d after the challenge.
32253039	11	80	theme	×	1765:1765	arg1	interaction					1773:1783	time × group interaction	1760:1783	time × group interaction	1760:1783	Data were evaluated using mixed models with time, group, and time × group interaction as fixed effects and cow as repeated subject.
32253039	15	81	theme	Milk	2269:2272	arg1	yield					2274:2278	Milk yield	2269:2278	Milk yield	2269:2278	Milk yield recovered within 2 d after the LPS-CH with no differences in morning milkings, whereas evening milk yield increased faster in HG.
32253039	10	82	theme	high-glucose	1595:1606	arg1	group					1608:1612	a high-glucose group	1593:1612	a high-glucose group (HG; 3.34-3.93 mmol/L, n = 7)	1593:1642	Based on plasma glucose concentrations in wk 1 to 4 after parturition before the LPS-CH, cows were retrospectively grouped into a high-glucose group (HG; 3.34-3.93 mmol/L, n = 7) and a low-glucose group (LG; 2.87-3.31 mmol/L, n = 8).
32253039	10	82	theme	high-glucose	1595:1606	arg1	HG					1615:1616	HG	1615:1616	HG	1615:1616	Based on plasma glucose concentrations in wk 1 to 4 after parturition before the LPS-CH, cows were retrospectively grouped into a high-glucose group (HG; 3.34-3.93 mmol/L, n = 7) and a low-glucose group (LG; 2.87-3.31 mmol/L, n = 8).
32253039	10	83	dep	wk	1507:1508	arg1	to					1512:1513	to	1512:1513	to	1512:1513	Based on plasma glucose concentrations in wk 1 to 4 after parturition before the LPS-CH, cows were retrospectively grouped into a high-glucose group (HG; 3.34-3.93 mmol/L, n = 7) and a low-glucose group (LG; 2.87-3.31 mmol/L, n = 8).
32253039	13	84	theme	fatty	2099:2103	arg1	acids					2105:2109	nonesterified fatty acids	2085:2109	nonesterified fatty acids	2085:2109	During LPS-CH, SCC and LDH increased similarly in HG and LG, body temperature increased less in HG, and BHB and nonesterified fatty acids were higher in LG compared with HG.
32253039	1	85	from	cows	193:196	arg1	Infections					152:161	Infections	152:161	Infections of the mammary gland in dairy cows	152:196	Infections of the mammary gland in dairy cows are commonly accompanied by reduced milk production and feed intake and poor milk quality.
32253039	16	86	from	LDH	2440:2442	arg1	milk					2471:2474	milk	2471:2474	milk	2471:2474	During 8 d after LPS-CH, SCC, LDH, IgG, and serum albumin in milk were lower in HG compared with LG.
32253039	11	87	theme	fixed	1788:1792	arg1	effects					1794:1800	fixed effects	1788:1800	fixed effects	1788:1800	Data were evaluated using mixed models with time, group, and time × group interaction as fixed effects and cow as repeated subject.
32253039	11	87	theme	fixed	1788:1792	arg1	models					1731:1736	mixed models	1725:1736	mixed models with time, group, and time × group interaction	1725:1783	Data were evaluated using mixed models with time, group, and time × group interaction as fixed effects and cow as repeated subject.
32253039	4	88	theme	yield	718:722	arg1	sampling					662:669	weekly blood sampling	649:669	weekly blood sampling	649:669	In 15 Holstein cows, weekly blood sampling and daily recording of dry matter intake, milk yield, milk composition, and body weight (to calculate energy balance) was started immediately after parturition.
32253039	4	88	theme	yield	718:722	arg1	recording					681:689	daily recording	675:689	daily recording	675:689	In 15 Holstein cows, weekly blood sampling and daily recording of dry matter intake, milk yield, milk composition, and body weight (to calculate energy balance) was started immediately after parturition.
32253039	0	89	theme	somatic	57:63	arg1	count					70:74	milk somatic cell count	52:74	milk somatic cell count	52:74	Metabolic status is associated with the recovery of milk somatic cell count and milk secretion after lipopolysaccharide-induced mastitis in dairy cows.
32253039	2	90	theme	cows	329:332	arg1	status					303:308	The metabolic status	289:308	The metabolic status of early-lactating cows	289:332	The metabolic status of early-lactating cows is known to affect immune response to pathogens and imposed immune challenges.
32253039	16	91	from	SCC	2435:2437	arg1	milk					2471:2474	milk	2471:2474	milk	2471:2474	During 8 d after LPS-CH, SCC, LDH, IgG, and serum albumin in milk were lower in HG compared with LG.
32253039	5	92	dep	LPS-CH	890:895	arg1	μg					901:902	50 μg	898:902	50 μg of LPS into 1 quarter	898:924	In wk 4 after parturition, cows underwent an intramammary LPS-CH (50 μg of LPS into 1 quarter).
32253039	0	93	theme	count	70:74	arg1	recovery					40:47	the recovery	36:47	the recovery of milk somatic cell count and milk secretion after lipopolysaccharide-induced mastitis in dairy cows	36:149	Metabolic status is associated with the recovery of milk somatic cell count and milk secretion after lipopolysaccharide-induced mastitis in dairy cows.
32253039	4	94	theme	blood	656:660	arg1	sampling					662:669	weekly blood sampling	649:669	weekly blood sampling	649:669	In 15 Holstein cows, weekly blood sampling and daily recording of dry matter intake, milk yield, milk composition, and body weight (to calculate energy balance) was started immediately after parturition.
32253039	0	95	theme	milk	80:83	arg1	secretion					85:93	milk secretion	80:93	milk secretion	80:93	Metabolic status is associated with the recovery of milk somatic cell count and milk secretion after lipopolysaccharide-induced mastitis in dairy cows.
32253039	4	96	theme	composition	730:740	arg1	sampling					662:669	weekly blood sampling	649:669	weekly blood sampling	649:669	In 15 Holstein cows, weekly blood sampling and daily recording of dry matter intake, milk yield, milk composition, and body weight (to calculate energy balance) was started immediately after parturition.
32253039	4	96	theme	composition	730:740	arg1	recording					681:689	daily recording	675:689	daily recording	675:689	In 15 Holstein cows, weekly blood sampling and daily recording of dry matter intake, milk yield, milk composition, and body weight (to calculate energy balance) was started immediately after parturition.
32253039	4	97	dep	sampling	662:669	arg1	calculate					763:771	calculate	763:771	to calculate energy balance	760:786	In 15 Holstein cows, weekly blood sampling and daily recording of dry matter intake, milk yield, milk composition, and body weight (to calculate energy balance) was started immediately after parturition.
32253039	16	98	from	IgG	2445:2447	arg1	milk					2471:2474	milk	2471:2474	milk	2471:2474	During 8 d after LPS-CH, SCC, LDH, IgG, and serum albumin in milk were lower in HG compared with LG.
32253039	7	99	theme	glucose	1080:1086	arg1	concentrations					1062:1075	Plasma concentrations	1055:1075	Plasma concentrations of glucose, nonesterified fatty acids, β-hydroxybutyrate (BHB), cortisol, and insulin	1055:1161	Plasma concentrations of glucose, nonesterified fatty acids, β-hydroxybutyrate (BHB), cortisol, and insulin were analyzed.
32253039	12	100	theme	energy	1933:1938	arg1	balance					1940:1946	energy balance	1933:1946	energy balance	1933:1946	Glucose was lower and BHB was higher in LG compared with HG before LPS-CH, whereas dry matter intake, energy balance, and SCC did not differ.
32253039	4	101	theme	milk	713:716	arg1	yield					718:722	milk yield	713:722	milk yield	713:722	In 15 Holstein cows, weekly blood sampling and daily recording of dry matter intake, milk yield, milk composition, and body weight (to calculate energy balance) was started immediately after parturition.
32253039	6	102	theme	30-	977:979	arg1	intervals					992:1000	30- and 60-min intervals	977:1000	30- and 60-min intervals	977:1000	Blood and milk samples were taken in parallel at 30- and 60-min intervals, respectively, until 10 h after the LPS application.
32253039	2	103	theme	immune	394:399	arg1	challenges					401:410	imposed immune challenges	386:410	imposed immune challenges	386:410	The metabolic status of early-lactating cows is known to affect immune response to pathogens and imposed immune challenges.
32253039	12	104	theme	matter	1918:1923	arg1	intake					1925:1930	dry matter intake	1914:1930	dry matter intake	1914:1930	Glucose was lower and BHB was higher in LG compared with HG before LPS-CH, whereas dry matter intake, energy balance, and SCC did not differ.
32253039	7	105	theme	Plasma	1055:1060	arg1	concentrations					1062:1075	Plasma concentrations	1055:1075	Plasma concentrations of glucose, nonesterified fatty acids, β-hydroxybutyrate (BHB), cortisol, and insulin	1055:1161	Plasma concentrations of glucose, nonesterified fatty acids, β-hydroxybutyrate (BHB), cortisol, and insulin were analyzed.
32253039	4	106	theme	dry	694:696	arg1	intake					705:710	dry matter intake	694:710	dry matter intake	694:710	In 15 Holstein cows, weekly blood sampling and daily recording of dry matter intake, milk yield, milk composition, and body weight (to calculate energy balance) was started immediately after parturition.
32253039	1	107	theme	poor	270:273	arg1	quality					280:286	poor milk quality	270:286	poor milk quality	270:286	Infections of the mammary gland in dairy cows are commonly accompanied by reduced milk production and feed intake and poor milk quality.
32253039	5	108	theme	intramammary	877:888	arg1	LPS-CH					890:895	an intramammary LPS-CH	874:895	an intramammary LPS-CH (50 μg of LPS into 1 quarter)	874:925	In wk 4 after parturition, cows underwent an intramammary LPS-CH (50 μg of LPS into 1 quarter).
32253039	4	109	theme	intake	705:710	arg1	sampling					662:669	weekly blood sampling	649:669	weekly blood sampling	649:669	In 15 Holstein cows, weekly blood sampling and daily recording of dry matter intake, milk yield, milk composition, and body weight (to calculate energy balance) was started immediately after parturition.
32253039	4	109	theme	intake	705:710	arg1	recording					681:689	daily recording	675:689	daily recording	675:689	In 15 Holstein cows, weekly blood sampling and daily recording of dry matter intake, milk yield, milk composition, and body weight (to calculate energy balance) was started immediately after parturition.
32253039	10	110	theme	glucose	1481:1487	arg1	concentrations					1489:1502	plasma glucose concentrations	1474:1502	plasma glucose concentrations in wk 1 to 4 after parturition before the LPS-CH	1474:1551	Based on plasma glucose concentrations in wk 1 to 4 after parturition before the LPS-CH, cows were retrospectively grouped into a high-glucose group (HG; 3.34-3.93 mmol/L, n = 7) and a low-glucose group (LG; 2.87-3.31 mmol/L, n = 8).
32253039	4	111	theme	weight	752:757	arg1	sampling					662:669	weekly blood sampling	649:669	weekly blood sampling	649:669	In 15 Holstein cows, weekly blood sampling and daily recording of dry matter intake, milk yield, milk composition, and body weight (to calculate energy balance) was started immediately after parturition.
32253039	4	111	theme	weight	752:757	arg1	recording					681:689	daily recording	675:689	daily recording	675:689	In 15 Holstein cows, weekly blood sampling and daily recording of dry matter intake, milk yield, milk composition, and body weight (to calculate energy balance) was started immediately after parturition.
32253039	2	112	theme	imposed	386:392	arg1	challenges					401:410	imposed immune challenges	386:410	imposed immune challenges	386:410	The metabolic status of early-lactating cows is known to affect immune response to pathogens and imposed immune challenges.
32253039	14	113	theme	Dry	2147:2149	arg1	intake					2158:2163	Dry matter intake	2147:2163	Dry matter intake	2147:2163	Dry matter intake declined in both groups during the day of the LPS-CH but recovered to prechallenge values faster in HG.
32253039	10	114	theme	low-glucose	1650:1660	arg1	LG					1669:1670	LG	1669:1670	LG	1669:1670	Based on plasma glucose concentrations in wk 1 to 4 after parturition before the LPS-CH, cows were retrospectively grouped into a high-glucose group (HG; 3.34-3.93 mmol/L, n = 7) and a low-glucose group (LG; 2.87-3.31 mmol/L, n = 8).
32253039	10	114	theme	low-glucose	1650:1660	arg1	group					1662:1666	a low-glucose group	1648:1666	a low-glucose group (LG; 2.87-3.31 mmol/L, n = 8)	1648:1696	Based on plasma glucose concentrations in wk 1 to 4 after parturition before the LPS-CH, cows were retrospectively grouped into a high-glucose group (HG; 3.34-3.93 mmol/L, n = 7) and a low-glucose group (LG; 2.87-3.31 mmol/L, n = 8).
32253039	3	115	theme	lipopolysaccharide	489:506	arg1	LPS-CH					525:530	LPS-CH	525:530	LPS-CH	525:530	We investigated the extent to which metabolic status before an intramammary lipopolysaccharide (LPS) challenge (LPS-CH) is associated with immune response, milk production, and feed intake and the recovery thereof.
32253039	3	115	theme	lipopolysaccharide	489:506	arg1	challenge					514:522	an intramammary lipopolysaccharide (LPS) challenge	473:522	an intramammary lipopolysaccharide (LPS) challenge (LPS-CH)	473:531	We investigated the extent to which metabolic status before an intramammary lipopolysaccharide (LPS) challenge (LPS-CH) is associated with immune response, milk production, and feed intake and the recovery thereof.
32253039	5	116	theme	LPS	907:909	arg1	μg					901:902	50 μg	898:902	50 μg of LPS into 1 quarter	898:924	In wk 4 after parturition, cows underwent an intramammary LPS-CH (50 μg of LPS into 1 quarter).
32253039	7	117	theme	cortisol	1141:1148	arg1	concentrations					1062:1075	Plasma concentrations	1055:1075	Plasma concentrations of glucose, nonesterified fatty acids, β-hydroxybutyrate (BHB), cortisol, and insulin	1055:1161	Plasma concentrations of glucose, nonesterified fatty acids, β-hydroxybutyrate (BHB), cortisol, and insulin were analyzed.
32253039	8	118	theme	lactate	1251:1257	arg1	dehydrogenase					1259:1271	lactate dehydrogenase	1251:1271	lactate dehydrogenase (LDH) activity	1251:1286	In milk, serum albumin, IgG concentration, somatic cell count (SCC), and lactate dehydrogenase (LDH) activity were determined.
32253039	8	118	theme	lactate	1251:1257	arg1	LDH					1274:1276	LDH	1274:1276	LDH	1274:1276	In milk, serum albumin, IgG concentration, somatic cell count (SCC), and lactate dehydrogenase (LDH) activity were determined.
32253039	0	119	from	mastitis	128:135	arg1	cows					146:149	dairy cows	140:149	dairy cows	140:149	Metabolic status is associated with the recovery of milk somatic cell count and milk secretion after lipopolysaccharide-induced mastitis in dairy cows.
32253039	16	120	from	LPS-CH	2427:2432	arg1	milk					2471:2474	milk	2471:2474	milk	2471:2474	During 8 d after LPS-CH, SCC, LDH, IgG, and serum albumin in milk were lower in HG compared with LG.
32253039	1	121	theme	milk	234:237	arg1	production					239:248	reduced milk production	226:248	reduced milk production	226:248	Infections of the mammary gland in dairy cows are commonly accompanied by reduced milk production and feed intake and poor milk quality.
33821278	4	0	theme	SARS-CoV-2	572:581	arg1	S					583:583	SARS-CoV-2 S	572:583	SARS-CoV-2 S	572:583	We found that virus-like particles produced by coexpression of SARS-CoV-2 S, M, E and N proteins contained spike glycoproteins that were extensively modified by complex carbohydrates.
33821278	4	0	theme	SARS-CoV-2	572:581	arg1	E					589:589	E	589:589	E	589:589	We found that virus-like particles produced by coexpression of SARS-CoV-2 S, M, E and N proteins contained spike glycoproteins that were extensively modified by complex carbohydrates.
33821278	4	0	theme	SARS-CoV-2	572:581	arg1	proteins					597:604	N proteins	595:604	N proteins	595:604	We found that virus-like particles produced by coexpression of SARS-CoV-2 S, M, E and N proteins contained spike glycoproteins that were extensively modified by complex carbohydrates.
33821278	4	0	theme	SARS-CoV-2	572:581	arg1	M					586:586	M	586:586	M	586:586	We found that virus-like particles produced by coexpression of SARS-CoV-2 S, M, E and N proteins contained spike glycoproteins that were extensively modified by complex carbohydrates.
33821278	3	1	theme	antibody	488:495	arg1	inhibition					497:506	antibody inhibition	488:506	antibody inhibition	488:506	Approximately 35% of the SARS-CoV-2 S glycoprotein consists of carbohydrate, which can influence virus infectivity and susceptibility to antibody inhibition.
33821278	7	2	theme	significant	1111:1121	arg1	Asn					1100:1102	Asn 234	1100:1106	Even Asn 234	1095:1106	Even Asn 234, a significant percentage of which is decorated by high-mannose glycans on soluble and virion S trimers, is predominantly modified in the Golgi by processed glycans.
33821278	7	2	theme	significant	1111:1121	arg1	percentage					1123:1132	a significant percentage	1109:1132	a significant percentage of which is decorated by high-mannose glycans on soluble and virion S trimers	1109:1210	Even Asn 234, a significant percentage of which is decorated by high-mannose glycans on soluble and virion S trimers, is predominantly modified in the Golgi by processed glycans.
33821278	6	3	theme	solubilized	900:910	arg1	glycoproteins					914:926	soluble or solubilized S glycoproteins	889:926	soluble or solubilized S glycoproteins modified to prevent proteolytic cleavage and to retain a prefusion conformation	889:1006	Compared with soluble or solubilized S glycoproteins modified to prevent proteolytic cleavage and to retain a prefusion conformation, more of the wild-type S glycoprotein N-linked glycans are processed to complex forms.
33821278	10	4	theme	other	1623:1627	arg1	variants					1646:1653	other natural cysteine variants	1623:1653	other natural cysteine variants	1623:1653	Unlike other natural cysteine variants, a Cys15Phe (C15F) mutant retained partial, but unstable, infectivity.
33821278	6	5	link	N-linked	1046:1053	arg1	glycans					1055:1061	the wild-type S glycoprotein N-linked glycans	1017:1061	the wild-type S glycoprotein N-linked glycans	1017:1061	Compared with soluble or solubilized S glycoproteins modified to prevent proteolytic cleavage and to retain a prefusion conformation, more of the wild-type S glycoprotein N-linked glycans are processed to complex forms.
33821278	6	6	theme	soluble	889:895	arg1	glycoproteins					914:926	soluble or solubilized S glycoproteins	889:926	soluble or solubilized S glycoproteins modified to prevent proteolytic cleavage and to retain a prefusion conformation	889:1006	Compared with soluble or solubilized S glycoproteins modified to prevent proteolytic cleavage and to retain a prefusion conformation, more of the wild-type S glycoprotein N-linked glycans are processed to complex forms.
33821278	9	7	link	O-linked	1438:1445	arg1	glycosylation					1447:1459	O-linked glycosylation	1438:1459	O-linked glycosylation	1438:1459	Viruses pseudotyped with natural variants of the serine/threonine residues implicated in O-linked glycosylation were generally infectious and exhibited sensitivity to neutralization by soluble ACE2 and convalescent antisera comparable to that of the wild-type virus.
33821278	5	8	theme	wild-type	770:778	arg1	trimer					806:811	a wild-type SARS-CoV-2 S glycoprotein trimer	768:811	a wild-type SARS-CoV-2 S glycoprotein trimer	768:811	We used a fucose-selective lectin to enrich the Golgi-resident fraction of a wild-type SARS-CoV-2 S glycoprotein trimer, and determined its glycosylation and disulfide bond profile.
33821278	2	9	gly	glycoprotein	243:254	arg1	target					269:274	the major target	259:274	the major target for neutralizing antibodies elicited by natural infection and by vaccines	259:348	The S glycoprotein is the major target for neutralizing antibodies elicited by natural infection and by vaccines.
33821278	2	9	gly	glycoprotein	243:254	arg1	glycoprotein					243:254	The S glycoprotein	237:254	The S glycoprotein	237:254	The S glycoprotein is the major target for neutralizing antibodies elicited by natural infection and by vaccines.
33821278	1	10	gly	glycoprotein	172:183	arg1	glycoprotein					172:183	its spike (S) glycoprotein	158:183	its spike (S) glycoprotein anchored in the viral membrane	158:214	The SARS-CoV-2 coronavirus, the etiologic agent of COVID-19, uses its spike (S) glycoprotein anchored in the viral membrane to enter host cells.
33821278	2	11	theme	S	241:241	arg1	target					269:274	the major target	259:274	the major target for neutralizing antibodies elicited by natural infection and by vaccines	259:348	The S glycoprotein is the major target for neutralizing antibodies elicited by natural infection and by vaccines.
33821278	2	11	theme	S	241:241	arg1	glycoprotein					243:254	The S glycoprotein	237:254	The S glycoprotein	237:254	The S glycoprotein is the major target for neutralizing antibodies elicited by natural infection and by vaccines.
33821278	5	12	theme	bond	861:864	arg1	profile					866:872	its glycosylation and disulfide bond profile	829:872	profile	866:872	We used a fucose-selective lectin to enrich the Golgi-resident fraction of a wild-type SARS-CoV-2 S glycoprotein trimer, and determined its glycosylation and disulfide bond profile.
33821278	10	13	theme	cysteine	1637:1644	arg1	variants					1646:1653	other natural cysteine variants	1623:1653	other natural cysteine variants	1623:1653	Unlike other natural cysteine variants, a Cys15Phe (C15F) mutant retained partial, but unstable, infectivity.
33821278	2	14	theme	major	263:267	arg1	target					269:274	the major target	259:274	the major target for neutralizing antibodies elicited by natural infection and by vaccines	259:348	The S glycoprotein is the major target for neutralizing antibodies elicited by natural infection and by vaccines.
33821278	2	14	theme	major	263:267	arg1	glycoprotein					243:254	The S glycoprotein	237:254	The S glycoprotein	237:254	The S glycoprotein is the major target for neutralizing antibodies elicited by natural infection and by vaccines.
33821278	4	15	theme	spike	616:620	arg1	glycoproteins					622:634	spike glycoproteins	616:634	spike glycoproteins that were extensively modified by complex carbohydrates	616:690	We found that virus-like particles produced by coexpression of SARS-CoV-2 S, M, E and N proteins contained spike glycoproteins that were extensively modified by complex carbohydrates.
33821278	10	16	theme	C15F	1668:1671	arg1	mutant					1674:1679	a Cys15Phe (C15F) mutant	1656:1679	a Cys15Phe (C15F) mutant	1656:1679	Unlike other natural cysteine variants, a Cys15Phe (C15F) mutant retained partial, but unstable, infectivity.
33821278	9	17	theme	O-linked	1438:1445	arg1	glycosylation					1447:1459	O-linked glycosylation	1438:1459	O-linked glycosylation	1438:1459	Viruses pseudotyped with natural variants of the serine/threonine residues implicated in O-linked glycosylation were generally infectious and exhibited sensitivity to neutralization by soluble ACE2 and convalescent antisera comparable to that of the wild-type virus.
33821278	6	18	theme	proteolytic	948:958	arg1	cleavage					960:967	proteolytic cleavage	948:967	proteolytic cleavage	948:967	Compared with soluble or solubilized S glycoproteins modified to prevent proteolytic cleavage and to retain a prefusion conformation, more of the wild-type S glycoprotein N-linked glycans are processed to complex forms.
33821278	10	19	theme	Cys15Phe	1658:1665	arg1	mutant					1674:1679	a Cys15Phe (C15F) mutant	1656:1679	a Cys15Phe (C15F) mutant	1656:1679	Unlike other natural cysteine variants, a Cys15Phe (C15F) mutant retained partial, but unstable, infectivity.
33821278	1	20	theme	etiologic	124:132	arg1	agent					134:138	the etiologic agent	120:138	the etiologic agent of COVID-19	120:150	The SARS-CoV-2 coronavirus, the etiologic agent of COVID-19, uses its spike (S) glycoprotein anchored in the viral membrane to enter host cells.
33821278	1	20	theme	etiologic	124:132	arg1	coronavirus					107:117	The SARS-CoV-2 coronavirus	92:117	The SARS-CoV-2 coronavirus	92:117	The SARS-CoV-2 coronavirus, the etiologic agent of COVID-19, uses its spike (S) glycoprotein anchored in the viral membrane to enter host cells.
33821278	9	21	theme	convalescent	1551:1562	arg1	antisera					1564:1571	convalescent antisera	1551:1571	convalescent antisera comparable to that of the wild-type virus	1551:1613	Viruses pseudotyped with natural variants of the serine/threonine residues implicated in O-linked glycosylation were generally infectious and exhibited sensitivity to neutralization by soluble ACE2 and convalescent antisera comparable to that of the wild-type virus.
33821278	6	22	theme	wild-type	1021:1029	arg1	glycans					1055:1061	the wild-type S glycoprotein N-linked glycans	1017:1061	the wild-type S glycoprotein N-linked glycans	1017:1061	Compared with soluble or solubilized S glycoproteins modified to prevent proteolytic cleavage and to retain a prefusion conformation, more of the wild-type S glycoprotein N-linked glycans are processed to complex forms.
33821278	8	23	theme	O-linked	1311:1318	arg1	glycosylation					1320:1332	O-linked glycosylation	1311:1332	O-linked glycosylation	1311:1332	Three incompletely occupied sites of O-linked glycosylation were detected.
33821278	11	24	theme	Golgi	1774:1778	arg1	processing					1780:1789	the Golgi processing	1770:1789	the Golgi processing of the native SARS-CoV-2 S glycoprotein	1770:1829	These findings enhance our understanding of the Golgi processing of the native SARS-CoV-2 S glycoprotein carbohydrates and could assist the design of interventions.
33821278	5	25	theme	Golgi-resident	741:754	arg1	fraction					756:763	the Golgi-resident fraction	737:763	the Golgi-resident fraction of a wild-type SARS-CoV-2 S glycoprotein trimer	737:811	We used a fucose-selective lectin to enrich the Golgi-resident fraction of a wild-type SARS-CoV-2 S glycoprotein trimer, and determined its glycosylation and disulfide bond profile.
33821278	0	26	theme	glycosylation	12:24	arg1	bonding					40:46	disulfide bonding	30:46	disulfide bonding of wild-type SARS-CoV-2	30:70	Analysis of glycosylation and disulfide bonding of wild-type SARS-CoV-2 spike glycoprotein.
33821278	0	26	theme	glycosylation	12:24	arg1	Analysis					0:7	Analysis	0:7	Analysis of glycosylation	0:24	Analysis of glycosylation and disulfide bonding of wild-type SARS-CoV-2 spike glycoprotein.
33821278	9	27	theme	comparable	1573:1582	arg1	antisera					1564:1571	convalescent antisera	1551:1571	convalescent antisera comparable to that of the wild-type virus	1551:1613	Viruses pseudotyped with natural variants of the serine/threonine residues implicated in O-linked glycosylation were generally infectious and exhibited sensitivity to neutralization by soluble ACE2 and convalescent antisera comparable to that of the wild-type virus.
33821278	11	28	theme	S	1816:1816	arg1	glycoprotein					1818:1829	the native SARS-CoV-2 S glycoprotein	1794:1829	the native SARS-CoV-2 S glycoprotein	1794:1829	These findings enhance our understanding of the Golgi processing of the native SARS-CoV-2 S glycoprotein carbohydrates and could assist the design of interventions.
33821278	11	29	gly	glycoprotein	1818:1829	arg1	glycoprotein					1818:1829	the native SARS-CoV-2 S glycoprotein	1794:1829	the native SARS-CoV-2 S glycoprotein	1794:1829	These findings enhance our understanding of the Golgi processing of the native SARS-CoV-2 S glycoprotein carbohydrates and could assist the design of interventions.
33821278	11	30	theme	native	1798:1803	arg1	glycoprotein					1818:1829	the native SARS-CoV-2 S glycoprotein	1794:1829	the native SARS-CoV-2 S glycoprotein	1794:1829	These findings enhance our understanding of the Golgi processing of the native SARS-CoV-2 S glycoprotein carbohydrates and could assist the design of interventions.
33821278	7	31	theme	S	1202:1202	arg1	trimers					1204:1210	soluble and virion S trimers	1183:1210	soluble and virion S trimers	1183:1210	Even Asn 234, a significant percentage of which is decorated by high-mannose glycans on soluble and virion S trimers, is predominantly modified in the Golgi by processed glycans.
33821278	6	32	theme	prefusion	985:993	arg1	conformation					995:1006	a prefusion conformation	983:1006	a prefusion conformation	983:1006	Compared with soluble or solubilized S glycoproteins modified to prevent proteolytic cleavage and to retain a prefusion conformation, more of the wild-type S glycoprotein N-linked glycans are processed to complex forms.
33821278	8	33	gly	glycosylation	1320:1332	arg2	glycosylation					1320:1332	O-linked glycosylation	1311:1332	O-linked glycosylation	1311:1332	Three incompletely occupied sites of O-linked glycosylation were detected.
33821278	8	33	gly	glycosylation	1320:1332	arg2	sites					1302:1306	Three incompletely occupied sites	1274:1306	Three incompletely occupied sites of O-linked glycosylation	1274:1332	Three incompletely occupied sites of O-linked glycosylation were detected.
33821278	6	34	theme	complex	1080:1086	arg1	forms					1088:1092	complex forms	1080:1092	complex forms	1080:1092	Compared with soluble or solubilized S glycoproteins modified to prevent proteolytic cleavage and to retain a prefusion conformation, more of the wild-type S glycoprotein N-linked glycans are processed to complex forms.
33821278	3	35	theme	glycoprotein	389:400	arg1	glycoprotein					389:400	the SARS-CoV-2 S glycoprotein	372:400	the SARS-CoV-2 S glycoprotein	372:400	Approximately 35% of the SARS-CoV-2 S glycoprotein consists of carbohydrate, which can influence virus infectivity and susceptibility to antibody inhibition.
33821278	3	35	theme	glycoprotein	389:400	arg1	%					367:367	Approximately 35%	351:367	Approximately 35% of the SARS-CoV-2 S glycoprotein	351:400	Approximately 35% of the SARS-CoV-2 S glycoprotein consists of carbohydrate, which can influence virus infectivity and susceptibility to antibody inhibition.
33821278	5	36	theme	trimer	806:811	arg1	fraction					756:763	the Golgi-resident fraction	737:763	the Golgi-resident fraction of a wild-type SARS-CoV-2 S glycoprotein trimer	737:811	We used a fucose-selective lectin to enrich the Golgi-resident fraction of a wild-type SARS-CoV-2 S glycoprotein trimer, and determined its glycosylation and disulfide bond profile.
33821278	1	37	theme	SARS-CoV-2	96:105	arg1	agent					134:138	the etiologic agent	120:138	the etiologic agent of COVID-19	120:150	The SARS-CoV-2 coronavirus, the etiologic agent of COVID-19, uses its spike (S) glycoprotein anchored in the viral membrane to enter host cells.
33821278	1	37	theme	SARS-CoV-2	96:105	arg1	coronavirus					107:117	The SARS-CoV-2 coronavirus	92:117	The SARS-CoV-2 coronavirus	92:117	The SARS-CoV-2 coronavirus, the etiologic agent of COVID-19, uses its spike (S) glycoprotein anchored in the viral membrane to enter host cells.
33821278	7	38	theme	high-mannose	1159:1170	arg1	glycans					1172:1178	high-mannose glycans	1159:1178	high-mannose glycans on soluble and virion S trimers	1159:1210	Even Asn 234, a significant percentage of which is decorated by high-mannose glycans on soluble and virion S trimers, is predominantly modified in the Golgi by processed glycans.
33821278	3	39	theme	SARS-CoV-2	376:385	arg1	glycoprotein					389:400	the SARS-CoV-2 S glycoprotein	372:400	the SARS-CoV-2 S glycoprotein	372:400	Approximately 35% of the SARS-CoV-2 S glycoprotein consists of carbohydrate, which can influence virus infectivity and susceptibility to antibody inhibition.
33821278	6	40	gly	glycoprotein	1033:1044	arg1	glycoprotein					1033:1044	the wild-type S glycoprotein N-linked glycans	1017:1061	the wild-type S glycoprotein N-linked glycans	1017:1061	Compared with soluble or solubilized S glycoproteins modified to prevent proteolytic cleavage and to retain a prefusion conformation, more of the wild-type S glycoprotein N-linked glycans are processed to complex forms.
33821278	10	41	theme	unstable	1703:1710	arg1	infectivity					1713:1723	partial, but unstable, infectivity	1690:1723	partial, but unstable, infectivity	1690:1723	Unlike other natural cysteine variants, a Cys15Phe (C15F) mutant retained partial, but unstable, infectivity.
33821278	5	42	theme	disulfide	851:859	arg1	profile					866:872	its glycosylation and disulfide bond profile	829:872	profile	866:872	We used a fucose-selective lectin to enrich the Golgi-resident fraction of a wild-type SARS-CoV-2 S glycoprotein trimer, and determined its glycosylation and disulfide bond profile.
33821278	0	43	theme	disulfide	30:38	arg1	bonding					40:46	disulfide bonding	30:46	disulfide bonding of wild-type SARS-CoV-2	30:70	Analysis of glycosylation and disulfide bonding of wild-type SARS-CoV-2 spike glycoprotein.
33821278	6	44	theme	glycoprotein	1033:1044	arg1	glycans					1055:1061	the wild-type S glycoprotein N-linked glycans	1017:1061	the wild-type S glycoprotein N-linked glycans	1017:1061	Compared with soluble or solubilized S glycoproteins modified to prevent proteolytic cleavage and to retain a prefusion conformation, more of the wild-type S glycoprotein N-linked glycans are processed to complex forms.
33821278	0	45	theme	SARS-CoV-2	61:70	arg1	bonding					40:46	disulfide bonding	30:46	disulfide bonding of wild-type SARS-CoV-2	30:70	Analysis of glycosylation and disulfide bonding of wild-type SARS-CoV-2 spike glycoprotein.
33821278	0	45	theme	SARS-CoV-2	61:70	arg1	Analysis					0:7	Analysis	0:7	Analysis of glycosylation	0:24	Analysis of glycosylation and disulfide bonding of wild-type SARS-CoV-2 spike glycoprotein.
33821278	2	46	theme	natural	316:322	arg1	infection					324:332	natural infection	316:332	natural infection	316:332	The S glycoprotein is the major target for neutralizing antibodies elicited by natural infection and by vaccines.
33821278	5	47	theme	S	791:791	arg1	trimer					806:811	a wild-type SARS-CoV-2 S glycoprotein trimer	768:811	a wild-type SARS-CoV-2 S glycoprotein trimer	768:811	We used a fucose-selective lectin to enrich the Golgi-resident fraction of a wild-type SARS-CoV-2 S glycoprotein trimer, and determined its glycosylation and disulfide bond profile.
33821278	3	48	theme	virus	448:452	arg1	infectivity					454:464	virus infectivity	448:464	virus infectivity	448:464	Approximately 35% of the SARS-CoV-2 S glycoprotein consists of carbohydrate, which can influence virus infectivity and susceptibility to antibody inhibition.
33821278	1	49	theme	viral	201:205	arg1	membrane					207:214	the viral membrane	197:214	the viral membrane	197:214	The SARS-CoV-2 coronavirus, the etiologic agent of COVID-19, uses its spike (S) glycoprotein anchored in the viral membrane to enter host cells.
33821278	7	50	theme	processed	1255:1263	arg1	glycans					1265:1271	processed glycans	1255:1271	processed glycans	1255:1271	Even Asn 234, a significant percentage of which is decorated by high-mannose glycans on soluble and virion S trimers, is predominantly modified in the Golgi by processed glycans.
33821278	9	51	theme	residues	1415:1422	arg1	variants					1382:1389	natural variants	1374:1389	natural variants of the serine/threonine residues implicated in O-linked glycosylation	1374:1459	Viruses pseudotyped with natural variants of the serine/threonine residues implicated in O-linked glycosylation were generally infectious and exhibited sensitivity to neutralization by soluble ACE2 and convalescent antisera comparable to that of the wild-type virus.
33821278	0	52	theme	wild-type	51:59	arg1	SARS-CoV-2					61:70	wild-type SARS-CoV-2	51:70	wild-type SARS-CoV-2	51:70	Analysis of glycosylation and disulfide bonding of wild-type SARS-CoV-2 spike glycoprotein.
33821278	4	53	gly	glycoproteins	622:634	arg1	glycoproteins					622:634	spike glycoproteins	616:634	spike glycoproteins that were extensively modified by complex carbohydrates	616:690	We found that virus-like particles produced by coexpression of SARS-CoV-2 S, M, E and N proteins contained spike glycoproteins that were extensively modified by complex carbohydrates.
33821278	1	54	theme	host	225:228	arg1	cells					230:234	host cells	225:234	host cells	225:234	The SARS-CoV-2 coronavirus, the etiologic agent of COVID-19, uses its spike (S) glycoprotein anchored in the viral membrane to enter host cells.
33821278	6	55	theme	S	912:912	arg1	glycoproteins					914:926	soluble or solubilized S glycoproteins	889:926	soluble or solubilized S glycoproteins modified to prevent proteolytic cleavage and to retain a prefusion conformation	889:1006	Compared with soluble or solubilized S glycoproteins modified to prevent proteolytic cleavage and to retain a prefusion conformation, more of the wild-type S glycoprotein N-linked glycans are processed to complex forms.
33821278	10	56	theme	natural	1629:1635	arg1	variants					1646:1653	other natural cysteine variants	1623:1653	other natural cysteine variants	1623:1653	Unlike other natural cysteine variants, a Cys15Phe (C15F) mutant retained partial, but unstable, infectivity.
33821278	4	57	theme	S	583:583	arg1	coexpression					556:567	coexpression	556:567	coexpression of SARS-CoV-2 S, M, E and N proteins	556:604	We found that virus-like particles produced by coexpression of SARS-CoV-2 S, M, E and N proteins contained spike glycoproteins that were extensively modified by complex carbohydrates.
33821278	1	58	theme	spike	162:166	arg1	glycoprotein					172:183	its spike (S) glycoprotein	158:183	its spike (S) glycoprotein anchored in the viral membrane	158:214	The SARS-CoV-2 coronavirus, the etiologic agent of COVID-19, uses its spike (S) glycoprotein anchored in the viral membrane to enter host cells.
33821278	8	59	link	O-linked	1311:1318	arg1	glycosylation					1320:1332	O-linked glycosylation	1311:1332	O-linked glycosylation	1311:1332	Three incompletely occupied sites of O-linked glycosylation were detected.
33821278	7	60	mod	modified	1230:1237	arg1	Asn					1100:1102	Asn 234	1100:1106	Even Asn 234	1095:1106	Even Asn 234, a significant percentage of which is decorated by high-mannose glycans on soluble and virion S trimers, is predominantly modified in the Golgi by processed glycans.
33821278	7	60	mod	modified	1230:1237	arg3	glycans					1265:1271	processed glycans	1255:1271	processed glycans	1255:1271	Even Asn 234, a significant percentage of which is decorated by high-mannose glycans on soluble and virion S trimers, is predominantly modified in the Golgi by processed glycans.
33821278	7	60	mod	modified	1230:1237	arg1	percentage					1123:1132	a significant percentage	1109:1132	a significant percentage of which is decorated by high-mannose glycans on soluble and virion S trimers	1109:1210	Even Asn 234, a significant percentage of which is decorated by high-mannose glycans on soluble and virion S trimers, is predominantly modified in the Golgi by processed glycans.
33821278	1	61	theme	S	169:169	arg1	glycoprotein					172:183	its spike (S) glycoprotein	158:183	its spike (S) glycoprotein anchored in the viral membrane	158:214	The SARS-CoV-2 coronavirus, the etiologic agent of COVID-19, uses its spike (S) glycoprotein anchored in the viral membrane to enter host cells.
33821278	4	62	theme	complex	670:676	arg1	carbohydrates					678:690	complex carbohydrates	670:690	complex carbohydrates	670:690	We found that virus-like particles produced by coexpression of SARS-CoV-2 S, M, E and N proteins contained spike glycoproteins that were extensively modified by complex carbohydrates.
33821278	4	63	theme	N	595:595	arg1	S					583:583	SARS-CoV-2 S	572:583	SARS-CoV-2 S	572:583	We found that virus-like particles produced by coexpression of SARS-CoV-2 S, M, E and N proteins contained spike glycoproteins that were extensively modified by complex carbohydrates.
33821278	4	63	theme	N	595:595	arg1	proteins					597:604	N proteins	595:604	N proteins	595:604	We found that virus-like particles produced by coexpression of SARS-CoV-2 S, M, E and N proteins contained spike glycoproteins that were extensively modified by complex carbohydrates.
33821278	5	64	used	used	696:699	arg2	We					693:694	We	693:694	We	693:694	We used a fucose-selective lectin to enrich the Golgi-resident fraction of a wild-type SARS-CoV-2 S glycoprotein trimer, and determined its glycosylation and disulfide bond profile.
33821278	7	65	theme	virion	1195:1200	arg1	trimers					1204:1210	soluble and virion S trimers	1183:1210	soluble and virion S trimers	1183:1210	Even Asn 234, a significant percentage of which is decorated by high-mannose glycans on soluble and virion S trimers, is predominantly modified in the Golgi by processed glycans.
33821278	9	66	theme	soluble	1534:1540	arg1	ACE2					1542:1545	soluble ACE2	1534:1545	soluble ACE2	1534:1545	Viruses pseudotyped with natural variants of the serine/threonine residues implicated in O-linked glycosylation were generally infectious and exhibited sensitivity to neutralization by soluble ACE2 and convalescent antisera comparable to that of the wild-type virus.
33821278	6	67	theme	S	1031:1031	arg1	glycans					1055:1061	the wild-type S glycoprotein N-linked glycans	1017:1061	the wild-type S glycoprotein N-linked glycans	1017:1061	Compared with soluble or solubilized S glycoproteins modified to prevent proteolytic cleavage and to retain a prefusion conformation, more of the wild-type S glycoprotein N-linked glycans are processed to complex forms.
33821278	8	68	theme	occupied	1293:1300	arg1	sites					1302:1306	Three incompletely occupied sites	1274:1306	Three incompletely occupied sites of O-linked glycosylation	1274:1332	Three incompletely occupied sites of O-linked glycosylation were detected.
33821278	8	68	theme	occupied	1293:1300	arg1	glycosylation					1320:1332	O-linked glycosylation	1311:1332	O-linked glycosylation	1311:1332	Three incompletely occupied sites of O-linked glycosylation were detected.
33821278	1	69	theme	COVID-19	143:150	arg1	agent					134:138	the etiologic agent	120:138	the etiologic agent of COVID-19	120:150	The SARS-CoV-2 coronavirus, the etiologic agent of COVID-19, uses its spike (S) glycoprotein anchored in the viral membrane to enter host cells.
33821278	1	69	theme	COVID-19	143:150	arg1	coronavirus					107:117	The SARS-CoV-2 coronavirus	92:117	The SARS-CoV-2 coronavirus	92:117	The SARS-CoV-2 coronavirus, the etiologic agent of COVID-19, uses its spike (S) glycoprotein anchored in the viral membrane to enter host cells.
33821278	6	70	gly	glycoproteins	914:926	arg1	glycoproteins					914:926	soluble or solubilized S glycoproteins	889:926	soluble or solubilized S glycoproteins modified to prevent proteolytic cleavage and to retain a prefusion conformation	889:1006	Compared with soluble or solubilized S glycoproteins modified to prevent proteolytic cleavage and to retain a prefusion conformation, more of the wild-type S glycoprotein N-linked glycans are processed to complex forms.
33821278	11	71	theme	processing	1780:1789	arg1	understanding					1753:1765	our understanding	1749:1765	our understanding of the Golgi processing of the native SARS-CoV-2 S glycoprotein	1749:1829	These findings enhance our understanding of the Golgi processing of the native SARS-CoV-2 S glycoprotein carbohydrates and could assist the design of interventions.
33821278	8	72	theme	glycosylation	1320:1332	arg1	sites					1302:1306	Three incompletely occupied sites	1274:1306	Three incompletely occupied sites of O-linked glycosylation	1274:1332	Three incompletely occupied sites of O-linked glycosylation were detected.
33821278	8	72	theme	glycosylation	1320:1332	arg1	glycosylation					1320:1332	O-linked glycosylation	1311:1332	O-linked glycosylation	1311:1332	Three incompletely occupied sites of O-linked glycosylation were detected.
33821278	11	73	theme	glycoprotein	1818:1829	arg1	processing					1780:1789	the Golgi processing	1770:1789	the Golgi processing of the native SARS-CoV-2 S glycoprotein	1770:1829	These findings enhance our understanding of the Golgi processing of the native SARS-CoV-2 S glycoprotein carbohydrates and could assist the design of interventions.
33821278	5	74	theme	fucose-selective	703:718	arg1	lectin					720:725	a fucose-selective lectin	701:725	a fucose-selective lectin	701:725	We used a fucose-selective lectin to enrich the Golgi-resident fraction of a wild-type SARS-CoV-2 S glycoprotein trimer, and determined its glycosylation and disulfide bond profile.
33821278	11	75	theme	SARS-CoV-2	1805:1814	arg1	glycoprotein					1818:1829	the native SARS-CoV-2 S glycoprotein	1794:1829	the native SARS-CoV-2 S glycoprotein	1794:1829	These findings enhance our understanding of the Golgi processing of the native SARS-CoV-2 S glycoprotein carbohydrates and could assist the design of interventions.
33821278	7	76	theme	soluble	1183:1189	arg1	trimers					1204:1210	soluble and virion S trimers	1183:1210	soluble and virion S trimers	1183:1210	Even Asn 234, a significant percentage of which is decorated by high-mannose glycans on soluble and virion S trimers, is predominantly modified in the Golgi by processed glycans.
33821278	0	77	gly	glycoprotein	78:89	arg1	glycoprotein					78:89	glycoprotein	78:89	glycoprotein	78:89	Analysis of glycosylation and disulfide bonding of wild-type SARS-CoV-2 spike glycoprotein.
33821278	7	78	from	glycans	1172:1178	arg1	trimers					1204:1210	soluble and virion S trimers	1183:1210	soluble and virion S trimers	1183:1210	Even Asn 234, a significant percentage of which is decorated by high-mannose glycans on soluble and virion S trimers, is predominantly modified in the Golgi by processed glycans.
33821278	10	79	theme	partial	1690:1696	arg1	infectivity					1713:1723	partial, but unstable, infectivity	1690:1723	partial, but unstable, infectivity	1690:1723	Unlike other natural cysteine variants, a Cys15Phe (C15F) mutant retained partial, but unstable, infectivity.
33821278	5	80	theme	glycoprotein	793:804	arg1	trimer					806:811	a wild-type SARS-CoV-2 S glycoprotein trimer	768:811	a wild-type SARS-CoV-2 S glycoprotein trimer	768:811	We used a fucose-selective lectin to enrich the Golgi-resident fraction of a wild-type SARS-CoV-2 S glycoprotein trimer, and determined its glycosylation and disulfide bond profile.
33821278	9	81	theme	wild-type	1599:1607	arg1	virus					1609:1613	the wild-type virus	1595:1613	the wild-type virus	1595:1613	Viruses pseudotyped with natural variants of the serine/threonine residues implicated in O-linked glycosylation were generally infectious and exhibited sensitivity to neutralization by soluble ACE2 and convalescent antisera comparable to that of the wild-type virus.
33821278	4	82	contain	contained	606:614	arg2	glycoproteins					622:634	spike glycoproteins	616:634	spike glycoproteins that were extensively modified by complex carbohydrates	616:690	We found that virus-like particles produced by coexpression of SARS-CoV-2 S, M, E and N proteins contained spike glycoproteins that were extensively modified by complex carbohydrates.
33821278	4	82	contain	contained	606:614	arg1	particles					534:542	virus-like particles	523:542	virus-like particles produced by coexpression of SARS-CoV-2 S, M, E and N proteins	523:604	We found that virus-like particles produced by coexpression of SARS-CoV-2 S, M, E and N proteins contained spike glycoproteins that were extensively modified by complex carbohydrates.
33821278	6	83	theme	N-linked	1046:1053	arg1	glycans					1055:1061	the wild-type S glycoprotein N-linked glycans	1017:1061	the wild-type S glycoprotein N-linked glycans	1017:1061	Compared with soluble or solubilized S glycoproteins modified to prevent proteolytic cleavage and to retain a prefusion conformation, more of the wild-type S glycoprotein N-linked glycans are processed to complex forms.
33821278	3	84	theme	S	387:387	arg1	glycoprotein					389:400	the SARS-CoV-2 S glycoprotein	372:400	the SARS-CoV-2 S glycoprotein	372:400	Approximately 35% of the SARS-CoV-2 S glycoprotein consists of carbohydrate, which can influence virus infectivity and susceptibility to antibody inhibition.
33821278	5	85	gly	glycoprotein	793:804	arg1	glycoprotein					793:804	a wild-type SARS-CoV-2 S glycoprotein trimer	768:811	a wild-type SARS-CoV-2 S glycoprotein trimer	768:811	We used a fucose-selective lectin to enrich the Golgi-resident fraction of a wild-type SARS-CoV-2 S glycoprotein trimer, and determined its glycosylation and disulfide bond profile.
33821278	5	86	theme	SARS-CoV-2	780:789	arg1	trimer					806:811	a wild-type SARS-CoV-2 S glycoprotein trimer	768:811	a wild-type SARS-CoV-2 S glycoprotein trimer	768:811	We used a fucose-selective lectin to enrich the Golgi-resident fraction of a wild-type SARS-CoV-2 S glycoprotein trimer, and determined its glycosylation and disulfide bond profile.
33821278	9	87	theme	natural	1374:1380	arg1	variants					1382:1389	natural variants	1374:1389	natural variants of the serine/threonine residues implicated in O-linked glycosylation	1374:1459	Viruses pseudotyped with natural variants of the serine/threonine residues implicated in O-linked glycosylation were generally infectious and exhibited sensitivity to neutralization by soluble ACE2 and convalescent antisera comparable to that of the wild-type virus.
33821278	4	88	theme	virus-like	523:532	arg1	particles					534:542	virus-like particles	523:542	virus-like particles produced by coexpression of SARS-CoV-2 S, M, E and N proteins	523:604	We found that virus-like particles produced by coexpression of SARS-CoV-2 S, M, E and N proteins contained spike glycoproteins that were extensively modified by complex carbohydrates.
33821278	4	89	mod	modified	658:665	arg3	carbohydrates					678:690	complex carbohydrates	670:690	complex carbohydrates	670:690	We found that virus-like particles produced by coexpression of SARS-CoV-2 S, M, E and N proteins contained spike glycoproteins that were extensively modified by complex carbohydrates.
33821278	4	89	mod	modified	658:665	arg1	glycoproteins					622:634	spike glycoproteins	616:634	spike glycoproteins that were extensively modified by complex carbohydrates	616:690	We found that virus-like particles produced by coexpression of SARS-CoV-2 S, M, E and N proteins contained spike glycoproteins that were extensively modified by complex carbohydrates.
33821278	9	90	theme	serine/threonine	1398:1413	arg1	residues					1415:1422	the serine/threonine residues	1394:1422	the serine/threonine residues implicated in O-linked glycosylation	1394:1459	Viruses pseudotyped with natural variants of the serine/threonine residues implicated in O-linked glycosylation were generally infectious and exhibited sensitivity to neutralization by soluble ACE2 and convalescent antisera comparable to that of the wild-type virus.
33821278	3	91	gly	glycoprotein	389:400	arg1	glycoprotein					389:400	the SARS-CoV-2 S glycoprotein	372:400	the SARS-CoV-2 S glycoprotein	372:400	Approximately 35% of the SARS-CoV-2 S glycoprotein consists of carbohydrate, which can influence virus infectivity and susceptibility to antibody inhibition.
33821278	11	92	theme	interventions	1876:1888	arg1	design					1866:1871	the design	1862:1871	the design of interventions	1862:1888	These findings enhance our understanding of the Golgi processing of the native SARS-CoV-2 S glycoprotein carbohydrates and could assist the design of interventions.
34155230	1	0	theme	stem	195:198	arg1	hPSCs					207:211	hPSCs	207:211	hPSCs	207:211	Current protocols for producing cerebellar neurons from human pluripotent stem cells (hPSCs) often rely on animal co-culture and mostly exist as monolayers, limiting their capability to recapitulate the complex processes in the developing cerebellum.
34155230	1	0	theme	stem	195:198	arg1	cells					200:204	human pluripotent stem cells	177:204	human pluripotent stem cells (hPSCs)	177:212	Current protocols for producing cerebellar neurons from human pluripotent stem cells (hPSCs) often rely on animal co-culture and mostly exist as monolayers, limiting their capability to recapitulate the complex processes in the developing cerebellum.
34155230	0	1	theme	cerebellar	99:108	arg1	organoids					110:118	pluripotent stem cell-derived cerebellar organoids	69:118	pluripotent stem cell-derived cerebellar organoids	69:118	High-resolution transcriptional landscape of xeno-free human induced pluripotent stem cell-derived cerebellar organoids.
34155230	3	2	theme	cell	755:758	arg1	types					760:764	major cerebellar cell types	738:764	major cerebellar cell types	738:764	Single-cell profiling followed by comparison to human and mouse cerebellar atlases revealed the presence and maturity of transcriptionally distinct populations encompassing major cerebellar cell types.
34155230	2	3	theme	development	552:562	arg1	hallmarks					520:528	hallmarks	520:528	hallmarks of in vivo cerebellar development	520:562	Here, we employed a robust method, without the need for mouse co-culture to generate three-dimensional cerebellar organoids from hPSCs that display hallmarks of in vivo cerebellar development.
34155230	5	4	theme	hPSC-derived	1135:1146	arg1	organoids					1159:1167	the hPSC-derived cerebellar organoids	1131:1167	the hPSC-derived cerebellar organoids	1131:1167	We identified enrichment of cerebellar disease genes in distinct cell populations in the hPSC-derived cerebellar organoids.
34155230	4	5	theme	relevant	1009:1016	arg1	programmes					1034:1043	developmentally relevant gene expression programmes	993:1043	developmentally relevant gene expression programmes	993:1043	Encapsulation with Matrigel aimed to provide more physiologically-relevant conditions through recapitulation of basement-membrane signalling, influenced both growth dynamics and cellular composition of the organoids, altering developmentally relevant gene expression programmes.
34155230	4	6	theme	cellular	945:952	arg1	composition					954:964	cellular composition	945:964	cellular composition	945:964	Encapsulation with Matrigel aimed to provide more physiologically-relevant conditions through recapitulation of basement-membrane signalling, influenced both growth dynamics and cellular composition of the organoids, altering developmentally relevant gene expression programmes.
34155230	5	7	theme	distinct	1102:1109	arg1	populations					1116:1126	distinct cell populations	1102:1126	distinct cell populations in the hPSC-derived cerebellar organoids	1102:1167	We identified enrichment of cerebellar disease genes in distinct cell populations in the hPSC-derived cerebellar organoids.
34155230	6	8	theme	associated	1302:1311	arg1	disorders					1313:1321	its associated disorders	1298:1321	its associated disorders	1298:1321	These findings ascertain xeno-free human cerebellar organoids as a unique model to gain insight into cerebellar development and its associated disorders.
34155230	2	9	theme	cerebellar	541:550	arg1	development					552:562	in vivo cerebellar development	533:562	in vivo cerebellar development	533:562	Here, we employed a robust method, without the need for mouse co-culture to generate three-dimensional cerebellar organoids from hPSCs that display hallmarks of in vivo cerebellar development.
34155230	4	10	with	Encapsulation	767:779	arg1	Matrigel					786:793	Matrigel	786:793	Matrigel	786:793	Encapsulation with Matrigel aimed to provide more physiologically-relevant conditions through recapitulation of basement-membrane signalling, influenced both growth dynamics and cellular composition of the organoids, altering developmentally relevant gene expression programmes.
34155230	1	11	from	processes	332:340	arg1	cerebellum					360:369	the developing cerebellum	345:369	the developing cerebellum	345:369	Current protocols for producing cerebellar neurons from human pluripotent stem cells (hPSCs) often rely on animal co-culture and mostly exist as monolayers, limiting their capability to recapitulate the complex processes in the developing cerebellum.
34155230	5	12	theme	cell	1111:1114	arg1	populations					1116:1126	distinct cell populations	1102:1126	distinct cell populations in the hPSC-derived cerebellar organoids	1102:1167	We identified enrichment of cerebellar disease genes in distinct cell populations in the hPSC-derived cerebellar organoids.
34155230	4	13	dep	provide	804:810	arg1	influenced					909:918	influenced	909:918	influenced both growth dynamics and cellular composition of the organoids	909:981	Encapsulation with Matrigel aimed to provide more physiologically-relevant conditions through recapitulation of basement-membrane signalling, influenced both growth dynamics and cellular composition of the organoids, altering developmentally relevant gene expression programmes.
34155230	5	14	theme	disease	1085:1091	arg1	genes					1093:1097	cerebellar disease genes	1074:1097	cerebellar disease genes	1074:1097	We identified enrichment of cerebellar disease genes in distinct cell populations in the hPSC-derived cerebellar organoids.
34155230	4	15	theme	basement-membrane	879:895	arg1	signalling					897:906	basement-membrane signalling	879:906	basement-membrane signalling	879:906	Encapsulation with Matrigel aimed to provide more physiologically-relevant conditions through recapitulation of basement-membrane signalling, influenced both growth dynamics and cellular composition of the organoids, altering developmentally relevant gene expression programmes.
34155230	2	16	theme	in	533:534	arg1	development					552:562	in vivo cerebellar development	533:562	in vivo cerebellar development	533:562	Here, we employed a robust method, without the need for mouse co-culture to generate three-dimensional cerebellar organoids from hPSCs that display hallmarks of in vivo cerebellar development.
34155230	2	17	from	hPSCs	501:505	arg1	organoids					486:494	three-dimensional cerebellar organoids	457:494	three-dimensional cerebellar organoids from hPSCs that display hallmarks of in vivo cerebellar development	457:562	Here, we employed a robust method, without the need for mouse co-culture to generate three-dimensional cerebellar organoids from hPSCs that display hallmarks of in vivo cerebellar development.
34155230	5	18	from	enrichment	1060:1069	arg1	populations					1116:1126	distinct cell populations	1102:1126	distinct cell populations in the hPSC-derived cerebellar organoids	1102:1167	We identified enrichment of cerebellar disease genes in distinct cell populations in the hPSC-derived cerebellar organoids.
34155230	3	19	theme	Single-cell	565:575	arg1	profiling					577:585	Single-cell profiling	565:585	Single-cell profiling followed by comparison to human and mouse cerebellar atlases	565:646	Single-cell profiling followed by comparison to human and mouse cerebellar atlases revealed the presence and maturity of transcriptionally distinct populations encompassing major cerebellar cell types.
34155230	6	20	theme	unique	1237:1242	arg1	organoids					1222:1230	xeno-free human cerebellar organoids	1195:1230	xeno-free human cerebellar organoids	1195:1230	These findings ascertain xeno-free human cerebellar organoids as a unique model to gain insight into cerebellar development and its associated disorders.
34155230	6	20	theme	unique	1237:1242	arg1	model					1244:1248	a unique model	1235:1248	a unique model to gain insight into cerebellar development and its associated disorders	1235:1321	These findings ascertain xeno-free human cerebellar organoids as a unique model to gain insight into cerebellar development and its associated disorders.
34155230	2	21	theme	three-dimensional	457:473	arg1	organoids					486:494	three-dimensional cerebellar organoids	457:494	three-dimensional cerebellar organoids from hPSCs that display hallmarks of in vivo cerebellar development	457:562	Here, we employed a robust method, without the need for mouse co-culture to generate three-dimensional cerebellar organoids from hPSCs that display hallmarks of in vivo cerebellar development.
34155230	4	22	theme	organoids	973:981	arg1	dynamics					932:939	growth dynamics	925:939	growth dynamics	925:939	Encapsulation with Matrigel aimed to provide more physiologically-relevant conditions through recapitulation of basement-membrane signalling, influenced both growth dynamics and cellular composition of the organoids, altering developmentally relevant gene expression programmes.
34155230	4	22	theme	organoids	973:981	arg1	composition					954:964	cellular composition	945:964	cellular composition	945:964	Encapsulation with Matrigel aimed to provide more physiologically-relevant conditions through recapitulation of basement-membrane signalling, influenced both growth dynamics and cellular composition of the organoids, altering developmentally relevant gene expression programmes.
34155230	5	23	theme	cerebellar	1074:1083	arg1	genes					1093:1097	cerebellar disease genes	1074:1097	cerebellar disease genes	1074:1097	We identified enrichment of cerebellar disease genes in distinct cell populations in the hPSC-derived cerebellar organoids.
34155230	0	24	theme	High-resolution	0:14	arg1	landscape					32:40	High-resolution transcriptional landscape	0:40	High-resolution transcriptional landscape of xeno-free human	0:59	High-resolution transcriptional landscape of xeno-free human induced pluripotent stem cell-derived cerebellar organoids.
34155230	1	25	theme	Current	121:127	arg1	protocols					129:137	Current protocols	121:137	Current protocols for producing cerebellar neurons from human pluripotent stem cells (hPSCs)	121:212	Current protocols for producing cerebellar neurons from human pluripotent stem cells (hPSCs) often rely on animal co-culture and mostly exist as monolayers, limiting their capability to recapitulate the complex processes in the developing cerebellum.
34155230	2	26	theme	robust	392:397	arg1	method					399:404	a robust method	390:404	a robust method	390:404	Here, we employed a robust method, without the need for mouse co-culture to generate three-dimensional cerebellar organoids from hPSCs that display hallmarks of in vivo cerebellar development.
34155230	5	27	theme	genes	1093:1097	arg1	enrichment					1060:1069	enrichment	1060:1069	enrichment of cerebellar disease genes in distinct cell populations in the hPSC-derived cerebellar organoids	1060:1167	We identified enrichment of cerebellar disease genes in distinct cell populations in the hPSC-derived cerebellar organoids.
34155230	2	28	theme	mouse	428:432	arg1	co-culture					434:443	mouse co-culture	428:443	mouse co-culture	428:443	Here, we employed a robust method, without the need for mouse co-culture to generate three-dimensional cerebellar organoids from hPSCs that display hallmarks of in vivo cerebellar development.
34155230	3	29	theme	human	613:617	arg1	atlases					640:646	human and mouse cerebellar atlases	613:646	human and mouse cerebellar atlases	613:646	Single-cell profiling followed by comparison to human and mouse cerebellar atlases revealed the presence and maturity of transcriptionally distinct populations encompassing major cerebellar cell types.
34155230	2	30	dep	in	533:534	arg1	vivo					536:539	vivo	536:539	vivo	536:539	Here, we employed a robust method, without the need for mouse co-culture to generate three-dimensional cerebellar organoids from hPSCs that display hallmarks of in vivo cerebellar development.
34155230	4	31	theme	physiologically-relevant	817:840	arg1	conditions					842:851	more physiologically-relevant conditions	812:851	more physiologically-relevant conditions	812:851	Encapsulation with Matrigel aimed to provide more physiologically-relevant conditions through recapitulation of basement-membrane signalling, influenced both growth dynamics and cellular composition of the organoids, altering developmentally relevant gene expression programmes.
34155230	6	32	theme	cerebellar	1211:1220	arg1	organoids					1222:1230	xeno-free human cerebellar organoids	1195:1230	xeno-free human cerebellar organoids	1195:1230	These findings ascertain xeno-free human cerebellar organoids as a unique model to gain insight into cerebellar development and its associated disorders.
34155230	6	32	theme	cerebellar	1211:1220	arg1	model					1244:1248	a unique model	1235:1248	a unique model to gain insight into cerebellar development and its associated disorders	1235:1321	These findings ascertain xeno-free human cerebellar organoids as a unique model to gain insight into cerebellar development and its associated disorders.
34155230	0	33	theme	human	55:59	arg1	landscape					32:40	High-resolution transcriptional landscape	0:40	High-resolution transcriptional landscape of xeno-free human	0:59	High-resolution transcriptional landscape of xeno-free human induced pluripotent stem cell-derived cerebellar organoids.
34155230	3	34	theme	distinct	704:711	arg1	populations					713:723	transcriptionally distinct populations	686:723	transcriptionally distinct populations encompassing major cerebellar cell types	686:764	Single-cell profiling followed by comparison to human and mouse cerebellar atlases revealed the presence and maturity of transcriptionally distinct populations encompassing major cerebellar cell types.
34155230	3	35	theme	cerebellar	629:638	arg1	atlases					640:646	human and mouse cerebellar atlases	613:646	human and mouse cerebellar atlases	613:646	Single-cell profiling followed by comparison to human and mouse cerebellar atlases revealed the presence and maturity of transcriptionally distinct populations encompassing major cerebellar cell types.
34155230	1	36	theme	animal	228:233	arg1	co-culture					235:244	animal co-culture	228:244	animal co-culture	228:244	Current protocols for producing cerebellar neurons from human pluripotent stem cells (hPSCs) often rely on animal co-culture and mostly exist as monolayers, limiting their capability to recapitulate the complex processes in the developing cerebellum.
34155230	1	37	theme	complex	324:330	arg1	processes					332:340	the complex processes	320:340	the complex processes in the developing cerebellum	320:369	Current protocols for producing cerebellar neurons from human pluripotent stem cells (hPSCs) often rely on animal co-culture and mostly exist as monolayers, limiting their capability to recapitulate the complex processes in the developing cerebellum.
34155230	4	38	theme	growth	925:930	arg1	dynamics					932:939	growth dynamics	925:939	growth dynamics	925:939	Encapsulation with Matrigel aimed to provide more physiologically-relevant conditions through recapitulation of basement-membrane signalling, influenced both growth dynamics and cellular composition of the organoids, altering developmentally relevant gene expression programmes.
34155230	6	39	theme	xeno-free	1195:1203	arg1	organoids					1222:1230	xeno-free human cerebellar organoids	1195:1230	xeno-free human cerebellar organoids	1195:1230	These findings ascertain xeno-free human cerebellar organoids as a unique model to gain insight into cerebellar development and its associated disorders.
34155230	6	39	theme	xeno-free	1195:1203	arg1	model					1244:1248	a unique model	1235:1248	a unique model to gain insight into cerebellar development and its associated disorders	1235:1321	These findings ascertain xeno-free human cerebellar organoids as a unique model to gain insight into cerebellar development and its associated disorders.
34155230	0	40	theme	pluripotent	69:79	arg1	organoids					110:118	pluripotent stem cell-derived cerebellar organoids	69:118	pluripotent stem cell-derived cerebellar organoids	69:118	High-resolution transcriptional landscape of xeno-free human induced pluripotent stem cell-derived cerebellar organoids.
34155230	4	41	theme	expression	1023:1032	arg1	programmes					1034:1043	developmentally relevant gene expression programmes	993:1043	developmentally relevant gene expression programmes	993:1043	Encapsulation with Matrigel aimed to provide more physiologically-relevant conditions through recapitulation of basement-membrane signalling, influenced both growth dynamics and cellular composition of the organoids, altering developmentally relevant gene expression programmes.
34155230	3	42	dep	presence	661:668	arg1	the					657:659	the	657:659	the	657:659	Single-cell profiling followed by comparison to human and mouse cerebellar atlases revealed the presence and maturity of transcriptionally distinct populations encompassing major cerebellar cell types.
34155230	2	43	theme	cerebellar	475:484	arg1	organoids					486:494	three-dimensional cerebellar organoids	457:494	three-dimensional cerebellar organoids from hPSCs that display hallmarks of in vivo cerebellar development	457:562	Here, we employed a robust method, without the need for mouse co-culture to generate three-dimensional cerebellar organoids from hPSCs that display hallmarks of in vivo cerebellar development.
34155230	4	44	theme	gene	1018:1021	arg1	programmes					1034:1043	developmentally relevant gene expression programmes	993:1043	developmentally relevant gene expression programmes	993:1043	Encapsulation with Matrigel aimed to provide more physiologically-relevant conditions through recapitulation of basement-membrane signalling, influenced both growth dynamics and cellular composition of the organoids, altering developmentally relevant gene expression programmes.
34155230	3	45	theme	major	738:742	arg1	types					760:764	major cerebellar cell types	738:764	major cerebellar cell types	738:764	Single-cell profiling followed by comparison to human and mouse cerebellar atlases revealed the presence and maturity of transcriptionally distinct populations encompassing major cerebellar cell types.
34155230	3	46	theme	mouse	623:627	arg1	atlases					640:646	human and mouse cerebellar atlases	613:646	human and mouse cerebellar atlases	613:646	Single-cell profiling followed by comparison to human and mouse cerebellar atlases revealed the presence and maturity of transcriptionally distinct populations encompassing major cerebellar cell types.
34155230	0	47	link	cell-derived	86:97	arg1	organoids					110:118	pluripotent stem cell-derived cerebellar organoids	69:118	pluripotent stem cell-derived cerebellar organoids	69:118	High-resolution transcriptional landscape of xeno-free human induced pluripotent stem cell-derived cerebellar organoids.
34155230	1	48	theme	cerebellar	153:162	arg1	neurons					164:170	cerebellar neurons	153:170	cerebellar neurons	153:170	Current protocols for producing cerebellar neurons from human pluripotent stem cells (hPSCs) often rely on animal co-culture and mostly exist as monolayers, limiting their capability to recapitulate the complex processes in the developing cerebellum.
34155230	3	49	theme	cerebellar	744:753	arg1	types					760:764	major cerebellar cell types	738:764	major cerebellar cell types	738:764	Single-cell profiling followed by comparison to human and mouse cerebellar atlases revealed the presence and maturity of transcriptionally distinct populations encompassing major cerebellar cell types.
34155230	6	50	theme	cerebellar	1271:1280	arg1	development					1282:1292	cerebellar development	1271:1292	cerebellar development	1271:1292	These findings ascertain xeno-free human cerebellar organoids as a unique model to gain insight into cerebellar development and its associated disorders.
34155230	3	51	theme	populations	713:723	arg1	maturity					674:681	maturity	674:681	maturity	674:681	Single-cell profiling followed by comparison to human and mouse cerebellar atlases revealed the presence and maturity of transcriptionally distinct populations encompassing major cerebellar cell types.
34155230	3	51	theme	populations	713:723	arg1	presence					661:668	presence	661:668	presence	661:668	Single-cell profiling followed by comparison to human and mouse cerebellar atlases revealed the presence and maturity of transcriptionally distinct populations encompassing major cerebellar cell types.
34155230	4	52	theme	signalling	897:906	arg1	recapitulation					861:874	recapitulation	861:874	recapitulation of basement-membrane signalling	861:906	Encapsulation with Matrigel aimed to provide more physiologically-relevant conditions through recapitulation of basement-membrane signalling, influenced both growth dynamics and cellular composition of the organoids, altering developmentally relevant gene expression programmes.
34155230	1	53	theme	pluripotent	183:193	arg1	hPSCs					207:211	hPSCs	207:211	hPSCs	207:211	Current protocols for producing cerebellar neurons from human pluripotent stem cells (hPSCs) often rely on animal co-culture and mostly exist as monolayers, limiting their capability to recapitulate the complex processes in the developing cerebellum.
34155230	1	53	theme	pluripotent	183:193	arg1	cells					200:204	human pluripotent stem cells	177:204	human pluripotent stem cells (hPSCs)	177:212	Current protocols for producing cerebellar neurons from human pluripotent stem cells (hPSCs) often rely on animal co-culture and mostly exist as monolayers, limiting their capability to recapitulate the complex processes in the developing cerebellum.
34155230	0	54	theme	cell-derived	86:97	arg1	organoids					110:118	pluripotent stem cell-derived cerebellar organoids	69:118	pluripotent stem cell-derived cerebellar organoids	69:118	High-resolution transcriptional landscape of xeno-free human induced pluripotent stem cell-derived cerebellar organoids.
34155230	5	55	theme	cerebellar	1148:1157	arg1	organoids					1159:1167	the hPSC-derived cerebellar organoids	1131:1167	the hPSC-derived cerebellar organoids	1131:1167	We identified enrichment of cerebellar disease genes in distinct cell populations in the hPSC-derived cerebellar organoids.
34155230	1	56	theme	human	177:181	arg1	hPSCs					207:211	hPSCs	207:211	hPSCs	207:211	Current protocols for producing cerebellar neurons from human pluripotent stem cells (hPSCs) often rely on animal co-culture and mostly exist as monolayers, limiting their capability to recapitulate the complex processes in the developing cerebellum.
34155230	1	56	theme	human	177:181	arg1	cells					200:204	human pluripotent stem cells	177:204	human pluripotent stem cells (hPSCs)	177:212	Current protocols for producing cerebellar neurons from human pluripotent stem cells (hPSCs) often rely on animal co-culture and mostly exist as monolayers, limiting their capability to recapitulate the complex processes in the developing cerebellum.
34155230	0	57	theme	stem	81:84	arg1	organoids					110:118	pluripotent stem cell-derived cerebellar organoids	69:118	pluripotent stem cell-derived cerebellar organoids	69:118	High-resolution transcriptional landscape of xeno-free human induced pluripotent stem cell-derived cerebellar organoids.
34155230	1	58	theme	developing	349:358	arg1	cerebellum					360:369	the developing cerebellum	345:369	the developing cerebellum	345:369	Current protocols for producing cerebellar neurons from human pluripotent stem cells (hPSCs) often rely on animal co-culture and mostly exist as monolayers, limiting their capability to recapitulate the complex processes in the developing cerebellum.
34155230	5	59	link	hPSC-derived	1135:1146	arg1	organoids					1159:1167	the hPSC-derived cerebellar organoids	1131:1167	the hPSC-derived cerebellar organoids	1131:1167	We identified enrichment of cerebellar disease genes in distinct cell populations in the hPSC-derived cerebellar organoids.
34155230	0	60	theme	transcriptional	16:30	arg1	landscape					32:40	High-resolution transcriptional landscape	0:40	High-resolution transcriptional landscape of xeno-free human	0:59	High-resolution transcriptional landscape of xeno-free human induced pluripotent stem cell-derived cerebellar organoids.
34155230	5	61	from	populations	1116:1126	arg1	organoids					1159:1167	the hPSC-derived cerebellar organoids	1131:1167	the hPSC-derived cerebellar organoids	1131:1167	We identified enrichment of cerebellar disease genes in distinct cell populations in the hPSC-derived cerebellar organoids.
34155230	6	62	theme	human	1205:1209	arg1	organoids					1222:1230	xeno-free human cerebellar organoids	1195:1230	xeno-free human cerebellar organoids	1195:1230	These findings ascertain xeno-free human cerebellar organoids as a unique model to gain insight into cerebellar development and its associated disorders.
34155230	6	62	theme	human	1205:1209	arg1	model					1244:1248	a unique model	1235:1248	a unique model to gain insight into cerebellar development and its associated disorders	1235:1321	These findings ascertain xeno-free human cerebellar organoids as a unique model to gain insight into cerebellar development and its associated disorders.
32732931	7	0	theme	rapid	1382:1386	arg1	modulation					1388:1397	rapid modulation	1382:1397	rapid modulation of microbiome composition and activity that could be beneficial for the host (i.e., increasing eubiosis or decreasing dysbiosis)	1382:1526	In conclusion, the addition of nitrate to oral communities led to rapid modulation of microbiome composition and activity that could be beneficial for the host (i.e., increasing eubiosis or decreasing dysbiosis).
32732931	6	1	theme	Rothia	957:962	arg1	levels					874:879	Significantly higher levels	853:879	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×)	853:970	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×) were detected in the nitrate condition already after 5 h (both p < 0.01), while several caries-associated genera (Streptococcus, Veillonella and Oribacterium) and halitosis- and periodontitis-associated genera (Porphyromonas, Fusobacterium, Leptotrichia, Prevotella, and Alloprevotella) were significantly reduced (p < 0.05 at 5 h and/or 9 h).
32732931	1	2	theme	nitrate	140:146	arg1	5-8 mM					164:169	5-8 mM	164:169	5-8 mM	164:169	The salivary glands actively concentrate plasma nitrate, leading to high salivary nitrate concentrations (5-8 mM) after a nitrate-rich vegetable meal.
32732931	1	2	theme	nitrate	140:146	arg1	concentrations					148:161	high salivary nitrate concentrations	126:161	high salivary nitrate concentrations (5-8 mM) after a nitrate-rich vegetable meal	126:206	The salivary glands actively concentrate plasma nitrate, leading to high salivary nitrate concentrations (5-8 mM) after a nitrate-rich vegetable meal.
32732931	6	3	theme	nitrate-reducing	911:926	arg1	Neisseria					935:943	the oral health-associated nitrate-reducing genera Neisseria	884:943	the oral health-associated nitrate-reducing genera Neisseria (3.1 ×)	884:951	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×) were detected in the nitrate condition already after 5 h (both p < 0.01), while several caries-associated genera (Streptococcus, Veillonella and Oribacterium) and halitosis- and periodontitis-associated genera (Porphyromonas, Fusobacterium, Leptotrichia, Prevotella, and Alloprevotella) were significantly reduced (p < 0.05 at 5 h and/or 9 h).
32732931	6	3	theme	nitrate-reducing	911:926	arg1	3.1 ×					946:950	3.1 ×	946:950	3.1 ×	946:950	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×) were detected in the nitrate condition already after 5 h (both p < 0.01), while several caries-associated genera (Streptococcus, Veillonella and Oribacterium) and halitosis- and periodontitis-associated genera (Porphyromonas, Fusobacterium, Leptotrichia, Prevotella, and Alloprevotella) were significantly reduced (p < 0.05 at 5 h and/or 9 h).
32732931	5	4	theme	observed	801:808	arg1	production					819:828	the observed ammonium production	797:828	the observed ammonium production	797:828	Nitrate did not affect biofilm growth significantly, but reduced lactate production, while increasing the observed ammonium production and pH (all p < 0.01).
32732931	5	5	theme	ammonium	810:817	arg1	production					819:828	the observed ammonium production	797:828	the observed ammonium production	797:828	Nitrate did not affect biofilm growth significantly, but reduced lactate production, while increasing the observed ammonium production and pH (all p < 0.01).
32732931	6	6	theme	health-associated	893:909	arg1	Neisseria					935:943	the oral health-associated nitrate-reducing genera Neisseria	884:943	the oral health-associated nitrate-reducing genera Neisseria (3.1 ×)	884:951	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×) were detected in the nitrate condition already after 5 h (both p < 0.01), while several caries-associated genera (Streptococcus, Veillonella and Oribacterium) and halitosis- and periodontitis-associated genera (Porphyromonas, Fusobacterium, Leptotrichia, Prevotella, and Alloprevotella) were significantly reduced (p < 0.05 at 5 h and/or 9 h).
32732931	6	6	theme	health-associated	893:909	arg1	3.1 ×					946:950	3.1 ×	946:950	3.1 ×	946:950	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×) were detected in the nitrate condition already after 5 h (both p < 0.01), while several caries-associated genera (Streptococcus, Veillonella and Oribacterium) and halitosis- and periodontitis-associated genera (Porphyromonas, Fusobacterium, Leptotrichia, Prevotella, and Alloprevotella) were significantly reduced (p < 0.05 at 5 h and/or 9 h).
32732931	6	7	dep	Porphyromonas	1183:1195	arg1	Prevotella					1227:1236	Prevotella	1227:1236	Prevotella	1227:1236	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×) were detected in the nitrate condition already after 5 h (both p < 0.01), while several caries-associated genera (Streptococcus, Veillonella and Oribacterium) and halitosis- and periodontitis-associated genera (Porphyromonas, Fusobacterium, Leptotrichia, Prevotella, and Alloprevotella) were significantly reduced (p < 0.05 at 5 h and/or 9 h).
32732931	6	7	dep	Porphyromonas	1183:1195	arg1	Alloprevotella					1243:1256	Alloprevotella	1243:1256	Alloprevotella	1243:1256	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×) were detected in the nitrate condition already after 5 h (both p < 0.01), while several caries-associated genera (Streptococcus, Veillonella and Oribacterium) and halitosis- and periodontitis-associated genera (Porphyromonas, Fusobacterium, Leptotrichia, Prevotella, and Alloprevotella) were significantly reduced (p < 0.05 at 5 h and/or 9 h).
32732931	6	7	dep	Porphyromonas	1183:1195	arg1	Leptotrichia					1213:1224	Leptotrichia	1213:1224	Leptotrichia	1213:1224	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×) were detected in the nitrate condition already after 5 h (both p < 0.01), while several caries-associated genera (Streptococcus, Veillonella and Oribacterium) and halitosis- and periodontitis-associated genera (Porphyromonas, Fusobacterium, Leptotrichia, Prevotella, and Alloprevotella) were significantly reduced (p < 0.05 at 5 h and/or 9 h).
32732931	6	8	from	5 h	1299:1301	arg1	p < 0.05					1287:1294	p < 0.05	1287:1294	p < 0.05 at 5 h and/or 9 h	1287:1312	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×) were detected in the nitrate condition already after 5 h (both p < 0.01), while several caries-associated genera (Streptococcus, Veillonella and Oribacterium) and halitosis- and periodontitis-associated genera (Porphyromonas, Fusobacterium, Leptotrichia, Prevotella, and Alloprevotella) were significantly reduced (p < 0.05 at 5 h and/or 9 h).
32732931	4	9	theme	rRNA	664:667	arg1	sequencing					683:692	16S rRNA gene Illumina sequencing	660:692	16S rRNA gene Illumina sequencing	660:692	Samples were taken at 5 h (most nitrate reduced) and 9 h (all nitrate reduced) of biofilm formation for ammonium, lactate and pH measurements, as well as 16S rRNA gene Illumina sequencing.
32732931	8	10	theme	potential	1570:1578	arg1	Nitrate					1529:1535	Nitrate	1529:1535	Nitrate	1529:1535	Nitrate should thus be investigated as a potential prebiotic for oral health.
32732931	8	10	theme	potential	1570:1578	arg1	prebiotic					1580:1588	a potential prebiotic	1568:1588	a potential prebiotic for oral health	1568:1604	Nitrate should thus be investigated as a potential prebiotic for oral health.
32732931	4	11	theme	most	533:536	arg1	nitrate					538:544	most nitrate	533:544	most nitrate	533:544	Samples were taken at 5 h (most nitrate reduced) and 9 h (all nitrate reduced) of biofilm formation for ammonium, lactate and pH measurements, as well as 16S rRNA gene Illumina sequencing.
32732931	7	12	theme	activity	1429:1436	arg1	modulation					1388:1397	rapid modulation	1382:1397	rapid modulation of microbiome composition and activity that could be beneficial for the host (i.e., increasing eubiosis or decreasing dysbiosis)	1382:1526	In conclusion, the addition of nitrate to oral communities led to rapid modulation of microbiome composition and activity that could be beneficial for the host (i.e., increasing eubiosis or decreasing dysbiosis).
32732931	6	13	theme	caries-associated	1060:1076	arg1	genera					1078:1083	several caries-associated genera	1052:1083	several caries-associated genera (Streptococcus, Veillonella and Oribacterium)	1052:1129	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×) were detected in the nitrate condition already after 5 h (both p < 0.01), while several caries-associated genera (Streptococcus, Veillonella and Oribacterium) and halitosis- and periodontitis-associated genera (Porphyromonas, Fusobacterium, Leptotrichia, Prevotella, and Alloprevotella) were significantly reduced (p < 0.05 at 5 h and/or 9 h).
32732931	4	14	theme	16S	660:662	arg1	sequencing					683:692	16S rRNA gene Illumina sequencing	660:692	16S rRNA gene Illumina sequencing	660:692	Samples were taken at 5 h (most nitrate reduced) and 9 h (all nitrate reduced) of biofilm formation for ammonium, lactate and pH measurements, as well as 16S rRNA gene Illumina sequencing.
32732931	8	15	theme	oral	1594:1597	arg1	health					1599:1604	oral health	1594:1604	oral health	1594:1604	Nitrate should thus be investigated as a potential prebiotic for oral health.
32732931	4	16	theme	pH	632:633	arg1	measurements					635:646	pH measurements	632:646	pH measurements	632:646	Samples were taken at 5 h (most nitrate reduced) and 9 h (all nitrate reduced) of biofilm formation for ammonium, lactate and pH measurements, as well as 16S rRNA gene Illumina sequencing.
32732931	6	17	located	detected	977:984	arg1	condition					1001:1009	the nitrate condition	989:1009	the nitrate condition	989:1009	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×) were detected in the nitrate condition already after 5 h (both p < 0.01), while several caries-associated genera (Streptococcus, Veillonella and Oribacterium) and halitosis- and periodontitis-associated genera (Porphyromonas, Fusobacterium, Leptotrichia, Prevotella, and Alloprevotella) were significantly reduced (p < 0.05 at 5 h and/or 9 h).
32732931	6	17	located	detected	977:984	arg2	levels					874:879	Significantly higher levels	853:879	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×)	853:970	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×) were detected in the nitrate condition already after 5 h (both p < 0.01), while several caries-associated genera (Streptococcus, Veillonella and Oribacterium) and halitosis- and periodontitis-associated genera (Porphyromonas, Fusobacterium, Leptotrichia, Prevotella, and Alloprevotella) were significantly reduced (p < 0.05 at 5 h and/or 9 h).
32732931	3	18	theme	donors	426:431	arg1	saliva					405:410	saliva	405:410	saliva of 12 healthy donors	405:431	To test this, saliva of 12 healthy donors was collected to grow in vitro biofilms with and without 6.5 mM nitrate.
32732931	2	19	theme	rapid	257:261	arg1	changes					263:269	rapid changes	257:269	rapid changes in structure and function of polymicrobial communities	257:324	Nitrate is an ecological factor that can induce rapid changes in structure and function of polymicrobial communities, but the effects on the oral microbiota have not been clarified.
32732931	5	20	theme	lactate	760:766	arg1	production					768:777	lactate production	760:777	lactate production	760:777	Nitrate did not affect biofilm growth significantly, but reduced lactate production, while increasing the observed ammonium production and pH (all p < 0.01).
32732931	7	21	dep	host	1471:1474	arg1	i.e.					1477:1480	i.e., increasing eubiosis or decreasing dysbiosis	1477:1525	i.e.	1477:1480	In conclusion, the addition of nitrate to oral communities led to rapid modulation of microbiome composition and activity that could be beneficial for the host (i.e., increasing eubiosis or decreasing dysbiosis).
32732931	6	22	theme	periodontitis-associated	1150:1173	arg1	genera					1175:1180	periodontitis-associated genera	1150:1180	periodontitis-associated genera	1150:1180	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×) were detected in the nitrate condition already after 5 h (both p < 0.01), while several caries-associated genera (Streptococcus, Veillonella and Oribacterium) and halitosis- and periodontitis-associated genera (Porphyromonas, Fusobacterium, Leptotrichia, Prevotella, and Alloprevotella) were significantly reduced (p < 0.05 at 5 h and/or 9 h).
32732931	2	23	from	effects	335:341	arg1	microbiota					355:364	the oral microbiota	346:364	the oral microbiota	346:364	Nitrate is an ecological factor that can induce rapid changes in structure and function of polymicrobial communities, but the effects on the oral microbiota have not been clarified.
32732931	1	24	theme	salivary	62:69	arg1	glands					71:76	The salivary glands	58:76	The salivary glands	58:76	The salivary glands actively concentrate plasma nitrate, leading to high salivary nitrate concentrations (5-8 mM) after a nitrate-rich vegetable meal.
32732931	7	25	theme	nitrate	1347:1353	arg1	addition					1335:1342	the addition	1331:1342	the addition of nitrate to oral communities	1331:1373	In conclusion, the addition of nitrate to oral communities led to rapid modulation of microbiome composition and activity that could be beneficial for the host (i.e., increasing eubiosis or decreasing dysbiosis).
32732931	7	26	theme	oral	1358:1361	arg1	communities					1363:1373	oral communities	1358:1373	oral communities	1358:1373	In conclusion, the addition of nitrate to oral communities led to rapid modulation of microbiome composition and activity that could be beneficial for the host (i.e., increasing eubiosis or decreasing dysbiosis).
32732931	4	27	theme	biofilm	588:594	arg1	formation					596:604	biofilm formation	588:604	biofilm formation	588:604	Samples were taken at 5 h (most nitrate reduced) and 9 h (all nitrate reduced) of biofilm formation for ammonium, lactate and pH measurements, as well as 16S rRNA gene Illumina sequencing.
32732931	2	28	theme	oral	350:353	arg1	microbiota					355:364	the oral microbiota	346:364	the oral microbiota	346:364	Nitrate is an ecological factor that can induce rapid changes in structure and function of polymicrobial communities, but the effects on the oral microbiota have not been clarified.
32732931	0	29	theme	potential	13:21	arg1	prebiotic					23:31	a potential prebiotic	11:31	a potential prebiotic for the oral microbiome	11:55	Nitrate as a potential prebiotic for the oral microbiome.
32732931	6	30	dep	detected	977:984	arg1	p < 0.01					1035:1042	p < 0.01	1035:1042	p < 0.01	1035:1042	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×) were detected in the nitrate condition already after 5 h (both p < 0.01), while several caries-associated genera (Streptococcus, Veillonella and Oribacterium) and halitosis- and periodontitis-associated genera (Porphyromonas, Fusobacterium, Leptotrichia, Prevotella, and Alloprevotella) were significantly reduced (p < 0.05 at 5 h and/or 9 h).
32732931	3	31	theme	healthy	418:424	arg1	donors					426:431	12 healthy donors	415:431	12 healthy donors	415:431	To test this, saliva of 12 healthy donors was collected to grow in vitro biofilms with and without 6.5 mM nitrate.
32732931	6	32	theme	higher	867:872	arg1	levels					874:879	Significantly higher levels	853:879	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×)	853:970	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×) were detected in the nitrate condition already after 5 h (both p < 0.01), while several caries-associated genera (Streptococcus, Veillonella and Oribacterium) and halitosis- and periodontitis-associated genera (Porphyromonas, Fusobacterium, Leptotrichia, Prevotella, and Alloprevotella) were significantly reduced (p < 0.05 at 5 h and/or 9 h).
32732931	4	33	theme	Illumina	674:681	arg1	sequencing					683:692	16S rRNA gene Illumina sequencing	660:692	16S rRNA gene Illumina sequencing	660:692	Samples were taken at 5 h (most nitrate reduced) and 9 h (all nitrate reduced) of biofilm formation for ammonium, lactate and pH measurements, as well as 16S rRNA gene Illumina sequencing.
32732931	6	34	from	9 h	1310:1312	arg1	p < 0.05					1287:1294	p < 0.05	1287:1294	p < 0.05 at 5 h and/or 9 h	1287:1312	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×) were detected in the nitrate condition already after 5 h (both p < 0.01), while several caries-associated genera (Streptococcus, Veillonella and Oribacterium) and halitosis- and periodontitis-associated genera (Porphyromonas, Fusobacterium, Leptotrichia, Prevotella, and Alloprevotella) were significantly reduced (p < 0.05 at 5 h and/or 9 h).
32732931	1	35	theme	plasma	99:104	arg1	nitrate					106:112	plasma nitrate	99:112	plasma nitrate	99:112	The salivary glands actively concentrate plasma nitrate, leading to high salivary nitrate concentrations (5-8 mM) after a nitrate-rich vegetable meal.
32732931	1	36	theme	nitrate-rich	180:191	arg1	meal					203:206	a nitrate-rich vegetable meal	178:206	a nitrate-rich vegetable meal	178:206	The salivary glands actively concentrate plasma nitrate, leading to high salivary nitrate concentrations (5-8 mM) after a nitrate-rich vegetable meal.
32732931	6	37	dep	genera	1078:1083	arg1	Oribacterium					1117:1128	Oribacterium	1117:1128	Oribacterium	1117:1128	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×) were detected in the nitrate condition already after 5 h (both p < 0.01), while several caries-associated genera (Streptococcus, Veillonella and Oribacterium) and halitosis- and periodontitis-associated genera (Porphyromonas, Fusobacterium, Leptotrichia, Prevotella, and Alloprevotella) were significantly reduced (p < 0.05 at 5 h and/or 9 h).
32732931	6	37	dep	genera	1078:1083	arg1	Veillonella					1101:1111	Veillonella	1101:1111	Veillonella	1101:1111	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×) were detected in the nitrate condition already after 5 h (both p < 0.01), while several caries-associated genera (Streptococcus, Veillonella and Oribacterium) and halitosis- and periodontitis-associated genera (Porphyromonas, Fusobacterium, Leptotrichia, Prevotella, and Alloprevotella) were significantly reduced (p < 0.05 at 5 h and/or 9 h).
32732931	7	38	theme	composition	1413:1423	arg1	modulation					1388:1397	rapid modulation	1382:1397	rapid modulation of microbiome composition and activity that could be beneficial for the host (i.e., increasing eubiosis or decreasing dysbiosis)	1382:1526	In conclusion, the addition of nitrate to oral communities led to rapid modulation of microbiome composition and activity that could be beneficial for the host (i.e., increasing eubiosis or decreasing dysbiosis).
32732931	1	39	theme	vegetable	193:201	arg1	meal					203:206	a nitrate-rich vegetable meal	178:206	a nitrate-rich vegetable meal	178:206	The salivary glands actively concentrate plasma nitrate, leading to high salivary nitrate concentrations (5-8 mM) after a nitrate-rich vegetable meal.
32732931	6	40	dep	p < 0.01	1035:1042	arg1	both					1030:1033	both	1030:1033	both	1030:1033	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×) were detected in the nitrate condition already after 5 h (both p < 0.01), while several caries-associated genera (Streptococcus, Veillonella and Oribacterium) and halitosis- and periodontitis-associated genera (Porphyromonas, Fusobacterium, Leptotrichia, Prevotella, and Alloprevotella) were significantly reduced (p < 0.05 at 5 h and/or 9 h).
32732931	4	41	dep	5 h	528:530	arg1	reduced					546:552	reduced	546:552	reduced	546:552	Samples were taken at 5 h (most nitrate reduced) and 9 h (all nitrate reduced) of biofilm formation for ammonium, lactate and pH measurements, as well as 16S rRNA gene Illumina sequencing.
32732931	5	42	theme	biofilm	718:724	arg1	growth					726:731	biofilm growth	718:731	biofilm growth	718:731	Nitrate did not affect biofilm growth significantly, but reduced lactate production, while increasing the observed ammonium production and pH (all p < 0.01).
32732931	0	43	theme	oral	41:44	arg1	microbiome					46:55	the oral microbiome	37:55	the oral microbiome	37:55	Nitrate as a potential prebiotic for the oral microbiome.
32732931	6	44	dep	halitosis-	1135:1144	arg1	Porphyromonas					1183:1195	Porphyromonas	1183:1195	Porphyromonas	1183:1195	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×) were detected in the nitrate condition already after 5 h (both p < 0.01), while several caries-associated genera (Streptococcus, Veillonella and Oribacterium) and halitosis- and periodontitis-associated genera (Porphyromonas, Fusobacterium, Leptotrichia, Prevotella, and Alloprevotella) were significantly reduced (p < 0.05 at 5 h and/or 9 h).
32732931	7	45	theme	microbiome	1402:1411	arg1	composition					1413:1423	microbiome composition	1402:1423	microbiome composition	1402:1423	In conclusion, the addition of nitrate to oral communities led to rapid modulation of microbiome composition and activity that could be beneficial for the host (i.e., increasing eubiosis or decreasing dysbiosis).
32732931	4	46	dep	9 h	559:561	arg1	reduced					576:582	reduced	576:582	reduced	576:582	Samples were taken at 5 h (most nitrate reduced) and 9 h (all nitrate reduced) of biofilm formation for ammonium, lactate and pH measurements, as well as 16S rRNA gene Illumina sequencing.
32732931	7	47	dep	i.e.	1477:1480	arg1	increasing					1483:1492	increasing	1483:1492	increasing eubiosis	1483:1501	In conclusion, the addition of nitrate to oral communities led to rapid modulation of microbiome composition and activity that could be beneficial for the host (i.e., increasing eubiosis or decreasing dysbiosis).
32732931	7	47	dep	i.e.	1477:1480	arg1	decreasing					1506:1515	decreasing	1506:1515	decreasing dysbiosis	1506:1525	In conclusion, the addition of nitrate to oral communities led to rapid modulation of microbiome composition and activity that could be beneficial for the host (i.e., increasing eubiosis or decreasing dysbiosis).
32732931	6	48	theme	several	1052:1058	arg1	genera					1078:1083	several caries-associated genera	1052:1083	several caries-associated genera (Streptococcus, Veillonella and Oribacterium)	1052:1129	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×) were detected in the nitrate condition already after 5 h (both p < 0.01), while several caries-associated genera (Streptococcus, Veillonella and Oribacterium) and halitosis- and periodontitis-associated genera (Porphyromonas, Fusobacterium, Leptotrichia, Prevotella, and Alloprevotella) were significantly reduced (p < 0.05 at 5 h and/or 9 h).
32732931	4	49	theme	formation	596:604	arg1	9 h					559:561	9 h	559:561	9 h (all nitrate reduced)	559:583	Samples were taken at 5 h (most nitrate reduced) and 9 h (all nitrate reduced) of biofilm formation for ammonium, lactate and pH measurements, as well as 16S rRNA gene Illumina sequencing.
32732931	4	49	theme	formation	596:604	arg1	5 h					528:530	5 h	528:530	5 h (most nitrate reduced)	528:553	Samples were taken at 5 h (most nitrate reduced) and 9 h (all nitrate reduced) of biofilm formation for ammonium, lactate and pH measurements, as well as 16S rRNA gene Illumina sequencing.
32732931	4	49	theme	formation	596:604	arg1	sequencing					683:692	16S rRNA gene Illumina sequencing	660:692	16S rRNA gene Illumina sequencing	660:692	Samples were taken at 5 h (most nitrate reduced) and 9 h (all nitrate reduced) of biofilm formation for ammonium, lactate and pH measurements, as well as 16S rRNA gene Illumina sequencing.
32732931	2	50	theme	communities	314:324	arg1	function					288:295	function	288:295	function	288:295	Nitrate is an ecological factor that can induce rapid changes in structure and function of polymicrobial communities, but the effects on the oral microbiota have not been clarified.
32732931	2	50	theme	communities	314:324	arg1	structure					274:282	structure	274:282	structure	274:282	Nitrate is an ecological factor that can induce rapid changes in structure and function of polymicrobial communities, but the effects on the oral microbiota have not been clarified.
32732931	4	51	theme	gene	669:672	arg1	sequencing					683:692	16S rRNA gene Illumina sequencing	660:692	16S rRNA gene Illumina sequencing	660:692	Samples were taken at 5 h (most nitrate reduced) and 9 h (all nitrate reduced) of biofilm formation for ammonium, lactate and pH measurements, as well as 16S rRNA gene Illumina sequencing.
32732931	3	52	theme	6.5 mM	490:495	arg1	nitrate					497:503	6.5 mM nitrate	490:503	6.5 mM nitrate	490:503	To test this, saliva of 12 healthy donors was collected to grow in vitro biofilms with and without 6.5 mM nitrate.
32732931	2	53	theme	polymicrobial	300:312	arg1	communities					314:324	polymicrobial communities	300:324	polymicrobial communities	300:324	Nitrate is an ecological factor that can induce rapid changes in structure and function of polymicrobial communities, but the effects on the oral microbiota have not been clarified.
32732931	6	54	theme	nitrate	993:999	arg1	condition					1001:1009	the nitrate condition	989:1009	the nitrate condition	989:1009	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×) were detected in the nitrate condition already after 5 h (both p < 0.01), while several caries-associated genera (Streptococcus, Veillonella and Oribacterium) and halitosis- and periodontitis-associated genera (Porphyromonas, Fusobacterium, Leptotrichia, Prevotella, and Alloprevotella) were significantly reduced (p < 0.05 at 5 h and/or 9 h).
32732931	1	55	theme	high	126:129	arg1	5-8 mM					164:169	5-8 mM	164:169	5-8 mM	164:169	The salivary glands actively concentrate plasma nitrate, leading to high salivary nitrate concentrations (5-8 mM) after a nitrate-rich vegetable meal.
32732931	1	55	theme	high	126:129	arg1	concentrations					148:161	high salivary nitrate concentrations	126:161	high salivary nitrate concentrations (5-8 mM) after a nitrate-rich vegetable meal	126:206	The salivary glands actively concentrate plasma nitrate, leading to high salivary nitrate concentrations (5-8 mM) after a nitrate-rich vegetable meal.
32732931	2	56	from	changes	263:269	arg1	function					288:295	function	288:295	function	288:295	Nitrate is an ecological factor that can induce rapid changes in structure and function of polymicrobial communities, but the effects on the oral microbiota have not been clarified.
32732931	2	56	from	changes	263:269	arg1	structure					274:282	structure	274:282	structure	274:282	Nitrate is an ecological factor that can induce rapid changes in structure and function of polymicrobial communities, but the effects on the oral microbiota have not been clarified.
32732931	6	57	theme	Neisseria	935:943	arg1	levels					874:879	Significantly higher levels	853:879	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×)	853:970	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×) were detected in the nitrate condition already after 5 h (both p < 0.01), while several caries-associated genera (Streptococcus, Veillonella and Oribacterium) and halitosis- and periodontitis-associated genera (Porphyromonas, Fusobacterium, Leptotrichia, Prevotella, and Alloprevotella) were significantly reduced (p < 0.05 at 5 h and/or 9 h).
32732931	6	58	theme	oral	888:891	arg1	Neisseria					935:943	the oral health-associated nitrate-reducing genera Neisseria	884:943	the oral health-associated nitrate-reducing genera Neisseria (3.1 ×)	884:951	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×) were detected in the nitrate condition already after 5 h (both p < 0.01), while several caries-associated genera (Streptococcus, Veillonella and Oribacterium) and halitosis- and periodontitis-associated genera (Porphyromonas, Fusobacterium, Leptotrichia, Prevotella, and Alloprevotella) were significantly reduced (p < 0.05 at 5 h and/or 9 h).
32732931	6	58	theme	oral	888:891	arg1	3.1 ×					946:950	3.1 ×	946:950	3.1 ×	946:950	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×) were detected in the nitrate condition already after 5 h (both p < 0.01), while several caries-associated genera (Streptococcus, Veillonella and Oribacterium) and halitosis- and periodontitis-associated genera (Porphyromonas, Fusobacterium, Leptotrichia, Prevotella, and Alloprevotella) were significantly reduced (p < 0.05 at 5 h and/or 9 h).
32732931	2	59	theme	ecological	223:232	arg1	factor					234:239	an ecological factor	220:239	an ecological factor that can induce rapid changes in structure and function of polymicrobial communities	220:324	Nitrate is an ecological factor that can induce rapid changes in structure and function of polymicrobial communities, but the effects on the oral microbiota have not been clarified.
32732931	2	59	theme	ecological	223:232	arg1	Nitrate					209:215	Nitrate	209:215	Nitrate	209:215	Nitrate is an ecological factor that can induce rapid changes in structure and function of polymicrobial communities, but the effects on the oral microbiota have not been clarified.
32732931	6	60	dep	reduced	1278:1284	arg1	p < 0.05					1287:1294	p < 0.05	1287:1294	p < 0.05 at 5 h and/or 9 h	1287:1312	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×) were detected in the nitrate condition already after 5 h (both p < 0.01), while several caries-associated genera (Streptococcus, Veillonella and Oribacterium) and halitosis- and periodontitis-associated genera (Porphyromonas, Fusobacterium, Leptotrichia, Prevotella, and Alloprevotella) were significantly reduced (p < 0.05 at 5 h and/or 9 h).
32732931	1	61	theme	salivary	131:138	arg1	5-8 mM					164:169	5-8 mM	164:169	5-8 mM	164:169	The salivary glands actively concentrate plasma nitrate, leading to high salivary nitrate concentrations (5-8 mM) after a nitrate-rich vegetable meal.
32732931	1	61	theme	salivary	131:138	arg1	concentrations					148:161	high salivary nitrate concentrations	126:161	high salivary nitrate concentrations (5-8 mM) after a nitrate-rich vegetable meal	126:206	The salivary glands actively concentrate plasma nitrate, leading to high salivary nitrate concentrations (5-8 mM) after a nitrate-rich vegetable meal.
32732931	6	62	theme	genera	928:933	arg1	Neisseria					935:943	the oral health-associated nitrate-reducing genera Neisseria	884:943	the oral health-associated nitrate-reducing genera Neisseria (3.1 ×)	884:951	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×) were detected in the nitrate condition already after 5 h (both p < 0.01), while several caries-associated genera (Streptococcus, Veillonella and Oribacterium) and halitosis- and periodontitis-associated genera (Porphyromonas, Fusobacterium, Leptotrichia, Prevotella, and Alloprevotella) were significantly reduced (p < 0.05 at 5 h and/or 9 h).
32732931	6	62	theme	genera	928:933	arg1	3.1 ×					946:950	3.1 ×	946:950	3.1 ×	946:950	Significantly higher levels of the oral health-associated nitrate-reducing genera Neisseria (3.1 ×) and Rothia (2.9 ×) were detected in the nitrate condition already after 5 h (both p < 0.01), while several caries-associated genera (Streptococcus, Veillonella and Oribacterium) and halitosis- and periodontitis-associated genera (Porphyromonas, Fusobacterium, Leptotrichia, Prevotella, and Alloprevotella) were significantly reduced (p < 0.05 at 5 h and/or 9 h).
33572881	6	0	with	women	1021:1025	arg1	obesity					1032:1038	obesity	1032:1038	obesity	1032:1038	Maternal obesity was associated with lower concentrations of several fucosylated and sialylated HMOs and infants born to women with obesity had lower intakes of these HMOs.
33572881	7	1	theme	total	1472:1476	arg1	HMOs					1485:1488	total acidic HMOs	1472:1488	total acidic HMOs	1472:1488	Maternal BMI was positively associated with lacto-N-neotetraose, 3-fucosyllactose, 3-sialyllactose and 6-sialyllactose and negatively associated with disialyllacto-N-tetraose, disialyllacto-N-hexaose, fucodisialyllacto-N-hexaose and total acidic HMOs concentrations at 2 M. Infant intakes of 3-fucosyllactose, 3-sialyllactose, 6-sialyllactose, disialyllacto-N-tetraose, disialyllacto-N-hexaose, and total acidic HMOs were positively associated with infant growth over the first 6 M of life.
33572881	3	2	dep	months	488:493	arg1	=					503:503	=	503:503	=	503:503	Human milk (HM) samples collected at 2 months (2 M; n = 194) postpartum were analyzed for HMO concentrations via high-performance liquid chromatography.
33572881	7	3	theme	first	1545:1549	arg1	M					1553:1553	the first 6 M	1541:1553	the first 6 M	1541:1553	Maternal BMI was positively associated with lacto-N-neotetraose, 3-fucosyllactose, 3-sialyllactose and 6-sialyllactose and negatively associated with disialyllacto-N-tetraose, disialyllacto-N-hexaose, fucodisialyllacto-N-hexaose and total acidic HMOs concentrations at 2 M. Infant intakes of 3-fucosyllactose, 3-sialyllactose, 6-sialyllactose, disialyllacto-N-tetraose, disialyllacto-N-hexaose, and total acidic HMOs were positively associated with infant growth over the first 6 M of life.
33572881	2	4	with	women	289:293	arg1	kg/m2					385:389	30.0-60.0 kg/m2	375:389	30.0-60.0 kg/m2	375:389	We aimed to quantify the composition of HMOs of women with normal weight (18.5-24.9 kg/m2), overweight (25.0-29.9 kg/m2), or obesity (30.0-60.0 kg/m2) and determine the effect of HMO intake on infant growth.
33572881	2	4	with	women	289:293	arg1	overweight					333:342	overweight	333:342	overweight (25.0-29.9 kg/m2)	333:360	We aimed to quantify the composition of HMOs of women with normal weight (18.5-24.9 kg/m2), overweight (25.0-29.9 kg/m2), or obesity (30.0-60.0 kg/m2) and determine the effect of HMO intake on infant growth.
33572881	2	4	with	women	289:293	arg1	obesity					366:372	obesity	366:372	obesity (30.0-60.0 kg/m2)	366:390	We aimed to quantify the composition of HMOs of women with normal weight (18.5-24.9 kg/m2), overweight (25.0-29.9 kg/m2), or obesity (30.0-60.0 kg/m2) and determine the effect of HMO intake on infant growth.
33572881	2	4	with	women	289:293	arg1	weight					307:312	normal weight	300:312	normal weight (18.5-24.9 kg/m2)	300:330	We aimed to quantify the composition of HMOs of women with normal weight (18.5-24.9 kg/m2), overweight (25.0-29.9 kg/m2), or obesity (30.0-60.0 kg/m2) and determine the effect of HMO intake on infant growth.
33572881	2	4	with	women	289:293	arg1	kg/m2					325:329	18.5-24.9 kg/m2	315:329	18.5-24.9 kg/m2	315:329	We aimed to quantify the composition of HMOs of women with normal weight (18.5-24.9 kg/m2), overweight (25.0-29.9 kg/m2), or obesity (30.0-60.0 kg/m2) and determine the effect of HMO intake on infant growth.
33572881	2	4	with	women	289:293	arg1	kg/m2					355:359	25.0-29.9 kg/m2	345:359	25.0-29.9 kg/m2	345:359	We aimed to quantify the composition of HMOs of women with normal weight (18.5-24.9 kg/m2), overweight (25.0-29.9 kg/m2), or obesity (30.0-60.0 kg/m2) and determine the effect of HMO intake on infant growth.
33572881	0	5	theme	Maternal	81:88	arg1	Overweight					90:99	Maternal Overweight	81:99	Maternal Overweight	81:99	Human Milk Oligosaccharide Concentrations and Infant Intakes Are Associated with Maternal Overweight and Obesity and Predict Infant Growth.
33572881	6	6	theme	several	961:967	arg1	HMOs					996:999	several fucosylated and sialylated HMOs	961:999	several fucosylated and sialylated HMOs	961:999	Maternal obesity was associated with lower concentrations of several fucosylated and sialylated HMOs and infants born to women with obesity had lower intakes of these HMOs.
33572881	3	7	theme	Human	449:453	arg1	HM					461:462	HM	461:462	HM	461:462	Human milk (HM) samples collected at 2 months (2 M; n = 194) postpartum were analyzed for HMO concentrations via high-performance liquid chromatography.
33572881	3	7	theme	Human	449:453	arg1	milk					455:458	Human milk	449:458	Human milk (HM) samples collected at 2 months (2 M; n = 194) postpartum	449:519	Human milk (HM) samples collected at 2 months (2 M; n = 194) postpartum were analyzed for HMO concentrations via high-performance liquid chromatography.
33572881	7	8	theme	M.	1344:1345	arg1	intakes					1354:1360	2 M. Infant intakes	1342:1360	2 M. Infant intakes of 3-fucosyllactose, 3-sialyllactose, 6-sialyllactose, disialyllacto-N-tetraose, disialyllacto-N-hexaose, and total acidic HMOs	1342:1488	Maternal BMI was positively associated with lacto-N-neotetraose, 3-fucosyllactose, 3-sialyllactose and 6-sialyllactose and negatively associated with disialyllacto-N-tetraose, disialyllacto-N-hexaose, fucodisialyllacto-N-hexaose and total acidic HMOs concentrations at 2 M. Infant intakes of 3-fucosyllactose, 3-sialyllactose, 6-sialyllactose, disialyllacto-N-tetraose, disialyllacto-N-hexaose, and total acidic HMOs were positively associated with infant growth over the first 6 M of life.
33572881	7	9	dep	concentrations	1324:1337	arg1	associated					1506:1515	associated	1506:1515	were positively associated with infant growth over the first 6 M of life	1490:1561	Maternal BMI was positively associated with lacto-N-neotetraose, 3-fucosyllactose, 3-sialyllactose and 6-sialyllactose and negatively associated with disialyllacto-N-tetraose, disialyllacto-N-hexaose, fucodisialyllacto-N-hexaose and total acidic HMOs concentrations at 2 M. Infant intakes of 3-fucosyllactose, 3-sialyllactose, 6-sialyllactose, disialyllacto-N-tetraose, disialyllacto-N-hexaose, and total acidic HMOs were positively associated with infant growth over the first 6 M of life.
33572881	2	10	theme	HMOs	281:284	arg1	composition					266:276	the composition	262:276	the composition of HMOs of women with normal weight (18.5-24.9 kg/m2), overweight (25.0-29.9 kg/m2), or obesity (30.0-60.0 kg/m2)	262:390	We aimed to quantify the composition of HMOs of women with normal weight (18.5-24.9 kg/m2), overweight (25.0-29.9 kg/m2), or obesity (30.0-60.0 kg/m2) and determine the effect of HMO intake on infant growth.
33572881	6	11	theme	lower	1044:1048	arg1	intakes					1050:1056	lower intakes	1044:1056	lower intakes of these HMOs	1044:1070	Maternal obesity was associated with lower concentrations of several fucosylated and sialylated HMOs and infants born to women with obesity had lower intakes of these HMOs.
33572881	3	12	theme	months	488:493	arg1	postpartum					510:519	2 months (2 M; n = 194) postpartum	486:519	2 months (2 M; n = 194) postpartum	486:519	Human milk (HM) samples collected at 2 months (2 M; n = 194) postpartum were analyzed for HMO concentrations via high-performance liquid chromatography.
33572881	2	13	from	effect	410:415	arg1	growth					441:446	infant growth	434:446	infant growth	434:446	We aimed to quantify the composition of HMOs of women with normal weight (18.5-24.9 kg/m2), overweight (25.0-29.9 kg/m2), or obesity (30.0-60.0 kg/m2) and determine the effect of HMO intake on infant growth.
33572881	5	14	theme	first	875:879	arg1	M					883:883	the first 6 M	871:883	the first 6 M	871:883	Linear regressions and linear mixed-effects models were conducted examining the relationships between maternal BMI and HMO composition and HMO intake and infant growth over the first 6 M, respectively.
33572881	4	15	theme	HM	609:610	arg1	intake					612:617	Infant HM intake	602:617	Infant HM intake	602:617	Infant HM intake, anthropometrics and body composition were assessed at 2 M and 6 M postpartum.
33572881	7	16	theme	life	1558:1561	arg1	growth					1529:1534	infant growth	1522:1534	infant growth over the first 6 M of life	1522:1561	Maternal BMI was positively associated with lacto-N-neotetraose, 3-fucosyllactose, 3-sialyllactose and 6-sialyllactose and negatively associated with disialyllacto-N-tetraose, disialyllacto-N-hexaose, fucodisialyllacto-N-hexaose and total acidic HMOs concentrations at 2 M. Infant intakes of 3-fucosyllactose, 3-sialyllactose, 6-sialyllactose, disialyllacto-N-tetraose, disialyllacto-N-hexaose, and total acidic HMOs were positively associated with infant growth over the first 6 M of life.
33572881	4	17	theme	Infant	602:607	arg1	intake					612:617	Infant HM intake	602:617	Infant HM intake	602:617	Infant HM intake, anthropometrics and body composition were assessed at 2 M and 6 M postpartum.
33572881	7	18	theme	acidic	1478:1483	arg1	HMOs					1485:1488	total acidic HMOs	1472:1488	total acidic HMOs	1472:1488	Maternal BMI was positively associated with lacto-N-neotetraose, 3-fucosyllactose, 3-sialyllactose and 6-sialyllactose and negatively associated with disialyllacto-N-tetraose, disialyllacto-N-hexaose, fucodisialyllacto-N-hexaose and total acidic HMOs concentrations at 2 M. Infant intakes of 3-fucosyllactose, 3-sialyllactose, 6-sialyllactose, disialyllacto-N-tetraose, disialyllacto-N-hexaose, and total acidic HMOs were positively associated with infant growth over the first 6 M of life.
33572881	5	19	theme	infant	852:857	arg1	growth					859:864	infant growth	852:864	infant growth	852:864	Linear regressions and linear mixed-effects models were conducted examining the relationships between maternal BMI and HMO composition and HMO intake and infant growth over the first 6 M, respectively.
33572881	6	20	theme	HMOs	996:999	arg1	concentrations					943:956	lower concentrations	937:956	lower concentrations of several fucosylated and sialylated HMOs	937:999	Maternal obesity was associated with lower concentrations of several fucosylated and sialylated HMOs and infants born to women with obesity had lower intakes of these HMOs.
33572881	5	21	theme	HMO	817:819	arg1	composition					821:831	HMO composition	817:831	HMO composition	817:831	Linear regressions and linear mixed-effects models were conducted examining the relationships between maternal BMI and HMO composition and HMO intake and infant growth over the first 6 M, respectively.
33572881	0	22	theme	Infant	46:51	arg1	Intakes					53:59	Infant Intakes	46:59	Infant Intakes	46:59	Human Milk Oligosaccharide Concentrations and Infant Intakes Are Associated with Maternal Overweight and Obesity and Predict Infant Growth.
33572881	6	23	theme	HMOs	1067:1070	arg1	intakes					1050:1056	lower intakes	1044:1056	lower intakes of these HMOs	1044:1070	Maternal obesity was associated with lower concentrations of several fucosylated and sialylated HMOs and infants born to women with obesity had lower intakes of these HMOs.
33572881	6	24	theme	sialylated	985:994	arg1	HMOs					996:999	several fucosylated and sialylated HMOs	961:999	several fucosylated and sialylated HMOs	961:999	Maternal obesity was associated with lower concentrations of several fucosylated and sialylated HMOs and infants born to women with obesity had lower intakes of these HMOs.
33572881	0	25	theme	Milk	6:9	arg1	Concentrations					27:40	Human Milk Oligosaccharide Concentrations	0:40	Human Milk Oligosaccharide Concentrations	0:40	Human Milk Oligosaccharide Concentrations and Infant Intakes Are Associated with Maternal Overweight and Obesity and Predict Infant Growth.
33572881	2	26	theme	intake	424:429	arg1	effect					410:415	the effect	406:415	the effect of HMO intake on infant growth	406:446	We aimed to quantify the composition of HMOs of women with normal weight (18.5-24.9 kg/m2), overweight (25.0-29.9 kg/m2), or obesity (30.0-60.0 kg/m2) and determine the effect of HMO intake on infant growth.
33572881	2	27	theme	normal	300:305	arg1	weight					307:312	normal weight	300:312	normal weight (18.5-24.9 kg/m2)	300:330	We aimed to quantify the composition of HMOs of women with normal weight (18.5-24.9 kg/m2), overweight (25.0-29.9 kg/m2), or obesity (30.0-60.0 kg/m2) and determine the effect of HMO intake on infant growth.
33572881	2	27	theme	normal	300:305	arg1	kg/m2					325:329	18.5-24.9 kg/m2	315:329	18.5-24.9 kg/m2	315:329	We aimed to quantify the composition of HMOs of women with normal weight (18.5-24.9 kg/m2), overweight (25.0-29.9 kg/m2), or obesity (30.0-60.0 kg/m2) and determine the effect of HMO intake on infant growth.
33572881	7	28	theme	Maternal	1073:1080	arg1	BMI					1082:1084	Maternal BMI	1073:1084	Maternal BMI	1073:1084	Maternal BMI was positively associated with lacto-N-neotetraose, 3-fucosyllactose, 3-sialyllactose and 6-sialyllactose and negatively associated with disialyllacto-N-tetraose, disialyllacto-N-hexaose, fucodisialyllacto-N-hexaose and total acidic HMOs concentrations at 2 M. Infant intakes of 3-fucosyllactose, 3-sialyllactose, 6-sialyllactose, disialyllacto-N-tetraose, disialyllacto-N-hexaose, and total acidic HMOs were positively associated with infant growth over the first 6 M of life.
33572881	1	29	theme	bioactive	179:187	arg1	oligosaccharides					151:166	Human milk oligosaccharides	140:166	Human milk oligosaccharides (HMOs)	140:173	Human milk oligosaccharides (HMOs) are bioactive molecules playing a critical role in infant health.
33572881	1	29	theme	bioactive	179:187	arg1	molecules					189:197	bioactive molecules	179:197	bioactive molecules playing a critical role in infant health	179:238	Human milk oligosaccharides (HMOs) are bioactive molecules playing a critical role in infant health.
33572881	0	30	theme	Human	0:4	arg1	Concentrations					27:40	Human Milk Oligosaccharide Concentrations	0:40	Human Milk Oligosaccharide Concentrations	0:40	Human Milk Oligosaccharide Concentrations and Infant Intakes Are Associated with Maternal Overweight and Obesity and Predict Infant Growth.
33572881	4	31	theme	body	640:643	arg1	composition					645:655	body composition	640:655	body composition	640:655	Infant HM intake, anthropometrics and body composition were assessed at 2 M and 6 M postpartum.
33572881	6	32	theme	Maternal	900:907	arg1	obesity					909:915	Maternal obesity	900:915	Maternal obesity	900:915	Maternal obesity was associated with lower concentrations of several fucosylated and sialylated HMOs and infants born to women with obesity had lower intakes of these HMOs.
33572881	6	33	theme	fucosylated	969:979	arg1	HMOs					996:999	several fucosylated and sialylated HMOs	961:999	several fucosylated and sialylated HMOs	961:999	Maternal obesity was associated with lower concentrations of several fucosylated and sialylated HMOs and infants born to women with obesity had lower intakes of these HMOs.
33572881	7	34	theme	6-sialyllactose	1400:1414	arg1	intakes					1354:1360	2 M. Infant intakes	1342:1360	2 M. Infant intakes of 3-fucosyllactose, 3-sialyllactose, 6-sialyllactose, disialyllacto-N-tetraose, disialyllacto-N-hexaose, and total acidic HMOs	1342:1488	Maternal BMI was positively associated with lacto-N-neotetraose, 3-fucosyllactose, 3-sialyllactose and 6-sialyllactose and negatively associated with disialyllacto-N-tetraose, disialyllacto-N-hexaose, fucodisialyllacto-N-hexaose and total acidic HMOs concentrations at 2 M. Infant intakes of 3-fucosyllactose, 3-sialyllactose, 6-sialyllactose, disialyllacto-N-tetraose, disialyllacto-N-hexaose, and total acidic HMOs were positively associated with infant growth over the first 6 M of life.
33572881	2	35	theme	women	289:293	arg1	composition					266:276	the composition	262:276	the composition of HMOs of women with normal weight (18.5-24.9 kg/m2), overweight (25.0-29.9 kg/m2), or obesity (30.0-60.0 kg/m2)	262:390	We aimed to quantify the composition of HMOs of women with normal weight (18.5-24.9 kg/m2), overweight (25.0-29.9 kg/m2), or obesity (30.0-60.0 kg/m2) and determine the effect of HMO intake on infant growth.
33572881	3	36	dep	=	503:503	arg1	M					498:498	2 M	496:498	2 M	496:498	Human milk (HM) samples collected at 2 months (2 M; n = 194) postpartum were analyzed for HMO concentrations via high-performance liquid chromatography.
33572881	0	37	theme	Oligosaccharide	11:25	arg1	Concentrations					27:40	Human Milk Oligosaccharide Concentrations	0:40	Human Milk Oligosaccharide Concentrations	0:40	Human Milk Oligosaccharide Concentrations and Infant Intakes Are Associated with Maternal Overweight and Obesity and Predict Infant Growth.
33572881	8	38	theme	Maternal	1564:1571	arg1	obesity					1573:1579	Maternal obesity	1564:1579	Maternal obesity	1564:1579	Maternal obesity is associated with changes in HMO concentrations that are associated with infant adiposity.
33572881	0	39	theme	Infant	125:130	arg1	Growth					132:137	Infant Growth	125:137	Infant Growth	125:137	Human Milk Oligosaccharide Concentrations and Infant Intakes Are Associated with Maternal Overweight and Obesity and Predict Infant Growth.
33572881	3	40	theme	2	486:486	arg1	months					488:493	months	488:493	months	488:493	Human milk (HM) samples collected at 2 months (2 M; n = 194) postpartum were analyzed for HMO concentrations via high-performance liquid chromatography.
33572881	3	41	theme	milk	455:458	arg1	samples					465:471	Human milk (HM) samples	449:471	Human milk (HM) samples collected at 2 months (2 M; n = 194) postpartum	449:519	Human milk (HM) samples collected at 2 months (2 M; n = 194) postpartum were analyzed for HMO concentrations via high-performance liquid chromatography.
33572881	2	42	theme	HMO	420:422	arg1	intake					424:429	HMO intake	420:429	HMO intake	420:429	We aimed to quantify the composition of HMOs of women with normal weight (18.5-24.9 kg/m2), overweight (25.0-29.9 kg/m2), or obesity (30.0-60.0 kg/m2) and determine the effect of HMO intake on infant growth.
33572881	3	43	theme	HMO	539:541	arg1	concentrations					543:556	HMO concentrations	539:556	HMO concentrations	539:556	Human milk (HM) samples collected at 2 months (2 M; n = 194) postpartum were analyzed for HMO concentrations via high-performance liquid chromatography.
33572881	7	44	theme	disialyllacto-N-hexaose	1443:1465	arg1	intakes					1354:1360	2 M. Infant intakes	1342:1360	2 M. Infant intakes of 3-fucosyllactose, 3-sialyllactose, 6-sialyllactose, disialyllacto-N-tetraose, disialyllacto-N-hexaose, and total acidic HMOs	1342:1488	Maternal BMI was positively associated with lacto-N-neotetraose, 3-fucosyllactose, 3-sialyllactose and 6-sialyllactose and negatively associated with disialyllacto-N-tetraose, disialyllacto-N-hexaose, fucodisialyllacto-N-hexaose and total acidic HMOs concentrations at 2 M. Infant intakes of 3-fucosyllactose, 3-sialyllactose, 6-sialyllactose, disialyllacto-N-tetraose, disialyllacto-N-hexaose, and total acidic HMOs were positively associated with infant growth over the first 6 M of life.
33572881	1	45	theme	critical	209:216	arg1	role					218:221	a critical role	207:221	a critical role	207:221	Human milk oligosaccharides (HMOs) are bioactive molecules playing a critical role in infant health.
33572881	7	46	theme	acidic	1312:1317	arg1	concentrations					1324:1337	total acidic HMOs concentrations	1306:1337	total acidic HMOs concentrations at 2 M. Infant intakes of 3-fucosyllactose, 3-sialyllactose, 6-sialyllactose, disialyllacto-N-tetraose, disialyllacto-N-hexaose, and total acidic HMOs were positively associated with infant growth over the first 6 M of life	1306:1561	Maternal BMI was positively associated with lacto-N-neotetraose, 3-fucosyllactose, 3-sialyllactose and 6-sialyllactose and negatively associated with disialyllacto-N-tetraose, disialyllacto-N-hexaose, fucodisialyllacto-N-hexaose and total acidic HMOs concentrations at 2 M. Infant intakes of 3-fucosyllactose, 3-sialyllactose, 6-sialyllactose, disialyllacto-N-tetraose, disialyllacto-N-hexaose, and total acidic HMOs were positively associated with infant growth over the first 6 M of life.
33572881	7	47	theme	HMOs	1485:1488	arg1	intakes					1354:1360	2 M. Infant intakes	1342:1360	2 M. Infant intakes of 3-fucosyllactose, 3-sialyllactose, 6-sialyllactose, disialyllacto-N-tetraose, disialyllacto-N-hexaose, and total acidic HMOs	1342:1488	Maternal BMI was positively associated with lacto-N-neotetraose, 3-fucosyllactose, 3-sialyllactose and 6-sialyllactose and negatively associated with disialyllacto-N-tetraose, disialyllacto-N-hexaose, fucodisialyllacto-N-hexaose and total acidic HMOs concentrations at 2 M. Infant intakes of 3-fucosyllactose, 3-sialyllactose, 6-sialyllactose, disialyllacto-N-tetraose, disialyllacto-N-hexaose, and total acidic HMOs were positively associated with infant growth over the first 6 M of life.
33572881	8	48	from	changes	1600:1606	arg1	concentrations					1615:1628	HMO concentrations	1611:1628	HMO concentrations that are associated with infant adiposity	1611:1670	Maternal obesity is associated with changes in HMO concentrations that are associated with infant adiposity.
33572881	7	49	theme	3-fucosyllactose	1365:1380	arg1	intakes					1354:1360	2 M. Infant intakes	1342:1360	2 M. Infant intakes of 3-fucosyllactose, 3-sialyllactose, 6-sialyllactose, disialyllacto-N-tetraose, disialyllacto-N-hexaose, and total acidic HMOs	1342:1488	Maternal BMI was positively associated with lacto-N-neotetraose, 3-fucosyllactose, 3-sialyllactose and 6-sialyllactose and negatively associated with disialyllacto-N-tetraose, disialyllacto-N-hexaose, fucodisialyllacto-N-hexaose and total acidic HMOs concentrations at 2 M. Infant intakes of 3-fucosyllactose, 3-sialyllactose, 6-sialyllactose, disialyllacto-N-tetraose, disialyllacto-N-hexaose, and total acidic HMOs were positively associated with infant growth over the first 6 M of life.
33572881	6	50	theme	lower	937:941	arg1	concentrations					943:956	lower concentrations	937:956	lower concentrations of several fucosylated and sialylated HMOs	937:999	Maternal obesity was associated with lower concentrations of several fucosylated and sialylated HMOs and infants born to women with obesity had lower intakes of these HMOs.
33572881	5	51	theme	maternal	800:807	arg1	BMI					809:811	maternal BMI	800:811	maternal BMI	800:811	Linear regressions and linear mixed-effects models were conducted examining the relationships between maternal BMI and HMO composition and HMO intake and infant growth over the first 6 M, respectively.
33572881	6	52	gly	sialylated	985:994	arg1	HMOs					996:999	several fucosylated and sialylated HMOs	961:999	several fucosylated and sialylated HMOs	961:999	Maternal obesity was associated with lower concentrations of several fucosylated and sialylated HMOs and infants born to women with obesity had lower intakes of these HMOs.
33572881	6	53	contain	had	1040:1042	arg1	infants					1005:1011	infants	1005:1011	infants born to women with obesity	1005:1038	Maternal obesity was associated with lower concentrations of several fucosylated and sialylated HMOs and infants born to women with obesity had lower intakes of these HMOs.
33572881	6	53	contain	had	1040:1042	arg2	intakes					1050:1056	lower intakes	1044:1056	lower intakes of these HMOs	1044:1070	Maternal obesity was associated with lower concentrations of several fucosylated and sialylated HMOs and infants born to women with obesity had lower intakes of these HMOs.
33572881	7	54	theme	Infant	1347:1352	arg1	intakes					1354:1360	2 M. Infant intakes	1342:1360	2 M. Infant intakes of 3-fucosyllactose, 3-sialyllactose, 6-sialyllactose, disialyllacto-N-tetraose, disialyllacto-N-hexaose, and total acidic HMOs	1342:1488	Maternal BMI was positively associated with lacto-N-neotetraose, 3-fucosyllactose, 3-sialyllactose and 6-sialyllactose and negatively associated with disialyllacto-N-tetraose, disialyllacto-N-hexaose, fucodisialyllacto-N-hexaose and total acidic HMOs concentrations at 2 M. Infant intakes of 3-fucosyllactose, 3-sialyllactose, 6-sialyllactose, disialyllacto-N-tetraose, disialyllacto-N-hexaose, and total acidic HMOs were positively associated with infant growth over the first 6 M of life.
33572881	7	55	theme	total	1306:1310	arg1	concentrations					1324:1337	total acidic HMOs concentrations	1306:1337	total acidic HMOs concentrations at 2 M. Infant intakes of 3-fucosyllactose, 3-sialyllactose, 6-sialyllactose, disialyllacto-N-tetraose, disialyllacto-N-hexaose, and total acidic HMOs were positively associated with infant growth over the first 6 M of life	1306:1561	Maternal BMI was positively associated with lacto-N-neotetraose, 3-fucosyllactose, 3-sialyllactose and 6-sialyllactose and negatively associated with disialyllacto-N-tetraose, disialyllacto-N-hexaose, fucodisialyllacto-N-hexaose and total acidic HMOs concentrations at 2 M. Infant intakes of 3-fucosyllactose, 3-sialyllactose, 6-sialyllactose, disialyllacto-N-tetraose, disialyllacto-N-hexaose, and total acidic HMOs were positively associated with infant growth over the first 6 M of life.
33572881	3	56	theme	high-performance	562:577	arg1	chromatography					586:599	high-performance liquid chromatography	562:599	high-performance liquid chromatography	562:599	Human milk (HM) samples collected at 2 months (2 M; n = 194) postpartum were analyzed for HMO concentrations via high-performance liquid chromatography.
33572881	6	57	gly	fucosylated	969:979	arg1	HMOs					996:999	several fucosylated and sialylated HMOs	961:999	several fucosylated and sialylated HMOs	961:999	Maternal obesity was associated with lower concentrations of several fucosylated and sialylated HMOs and infants born to women with obesity had lower intakes of these HMOs.
33572881	3	58	theme	liquid	579:584	arg1	chromatography					586:599	high-performance liquid chromatography	562:599	high-performance liquid chromatography	562:599	Human milk (HM) samples collected at 2 months (2 M; n = 194) postpartum were analyzed for HMO concentrations via high-performance liquid chromatography.
33572881	4	59	theme	M	684:684	arg1	postpartum					686:695	6 M postpartum	682:695	6 M postpartum	682:695	Infant HM intake, anthropometrics and body composition were assessed at 2 M and 6 M postpartum.
33572881	5	60	theme	HMO	837:839	arg1	intake					841:846	HMO intake	837:846	HMO intake	837:846	Linear regressions and linear mixed-effects models were conducted examining the relationships between maternal BMI and HMO composition and HMO intake and infant growth over the first 6 M, respectively.
33572881	8	61	theme	infant	1655:1660	arg1	adiposity					1662:1670	infant adiposity	1655:1670	infant adiposity	1655:1670	Maternal obesity is associated with changes in HMO concentrations that are associated with infant adiposity.
33572881	7	62	theme	3-sialyllactose	1383:1397	arg1	intakes					1354:1360	2 M. Infant intakes	1342:1360	2 M. Infant intakes of 3-fucosyllactose, 3-sialyllactose, 6-sialyllactose, disialyllacto-N-tetraose, disialyllacto-N-hexaose, and total acidic HMOs	1342:1488	Maternal BMI was positively associated with lacto-N-neotetraose, 3-fucosyllactose, 3-sialyllactose and 6-sialyllactose and negatively associated with disialyllacto-N-tetraose, disialyllacto-N-hexaose, fucodisialyllacto-N-hexaose and total acidic HMOs concentrations at 2 M. Infant intakes of 3-fucosyllactose, 3-sialyllactose, 6-sialyllactose, disialyllacto-N-tetraose, disialyllacto-N-hexaose, and total acidic HMOs were positively associated with infant growth over the first 6 M of life.
33572881	7	63	theme	disialyllacto-N-tetraose	1417:1440	arg1	intakes					1354:1360	2 M. Infant intakes	1342:1360	2 M. Infant intakes of 3-fucosyllactose, 3-sialyllactose, 6-sialyllactose, disialyllacto-N-tetraose, disialyllacto-N-hexaose, and total acidic HMOs	1342:1488	Maternal BMI was positively associated with lacto-N-neotetraose, 3-fucosyllactose, 3-sialyllactose and 6-sialyllactose and negatively associated with disialyllacto-N-tetraose, disialyllacto-N-hexaose, fucodisialyllacto-N-hexaose and total acidic HMOs concentrations at 2 M. Infant intakes of 3-fucosyllactose, 3-sialyllactose, 6-sialyllactose, disialyllacto-N-tetraose, disialyllacto-N-hexaose, and total acidic HMOs were positively associated with infant growth over the first 6 M of life.
33572881	4	64	theme	6	682:682	arg1	M					684:684	M	684:684	M	684:684	Infant HM intake, anthropometrics and body composition were assessed at 2 M and 6 M postpartum.
33572881	1	65	theme	Human	140:144	arg1	oligosaccharides					151:166	Human milk oligosaccharides	140:166	Human milk oligosaccharides (HMOs)	140:173	Human milk oligosaccharides (HMOs) are bioactive molecules playing a critical role in infant health.
33572881	1	65	theme	Human	140:144	arg1	molecules					189:197	bioactive molecules	179:197	bioactive molecules playing a critical role in infant health	179:238	Human milk oligosaccharides (HMOs) are bioactive molecules playing a critical role in infant health.
33572881	1	65	theme	Human	140:144	arg1	HMOs					169:172	HMOs	169:172	HMOs	169:172	Human milk oligosaccharides (HMOs) are bioactive molecules playing a critical role in infant health.
33572881	1	66	theme	infant	226:231	arg1	health					233:238	infant health	226:238	infant health	226:238	Human milk oligosaccharides (HMOs) are bioactive molecules playing a critical role in infant health.
33572881	7	67	theme	infant	1522:1527	arg1	growth					1529:1534	infant growth	1522:1534	infant growth over the first 6 M of life	1522:1561	Maternal BMI was positively associated with lacto-N-neotetraose, 3-fucosyllactose, 3-sialyllactose and 6-sialyllactose and negatively associated with disialyllacto-N-tetraose, disialyllacto-N-hexaose, fucodisialyllacto-N-hexaose and total acidic HMOs concentrations at 2 M. Infant intakes of 3-fucosyllactose, 3-sialyllactose, 6-sialyllactose, disialyllacto-N-tetraose, disialyllacto-N-hexaose, and total acidic HMOs were positively associated with infant growth over the first 6 M of life.
33572881	5	68	theme	mixed-effects	728:740	arg1	models					742:747	linear mixed-effects models	721:747	linear mixed-effects models	721:747	Linear regressions and linear mixed-effects models were conducted examining the relationships between maternal BMI and HMO composition and HMO intake and infant growth over the first 6 M, respectively.
33572881	1	69	theme	milk	146:149	arg1	oligosaccharides					151:166	Human milk oligosaccharides	140:166	Human milk oligosaccharides (HMOs)	140:173	Human milk oligosaccharides (HMOs) are bioactive molecules playing a critical role in infant health.
33572881	1	69	theme	milk	146:149	arg1	molecules					189:197	bioactive molecules	179:197	bioactive molecules playing a critical role in infant health	179:238	Human milk oligosaccharides (HMOs) are bioactive molecules playing a critical role in infant health.
33572881	1	69	theme	milk	146:149	arg1	HMOs					169:172	HMOs	169:172	HMOs	169:172	Human milk oligosaccharides (HMOs) are bioactive molecules playing a critical role in infant health.
33572881	7	70	theme	HMOs	1319:1322	arg1	concentrations					1324:1337	total acidic HMOs concentrations	1306:1337	total acidic HMOs concentrations at 2 M. Infant intakes of 3-fucosyllactose, 3-sialyllactose, 6-sialyllactose, disialyllacto-N-tetraose, disialyllacto-N-hexaose, and total acidic HMOs were positively associated with infant growth over the first 6 M of life	1306:1561	Maternal BMI was positively associated with lacto-N-neotetraose, 3-fucosyllactose, 3-sialyllactose and 6-sialyllactose and negatively associated with disialyllacto-N-tetraose, disialyllacto-N-hexaose, fucodisialyllacto-N-hexaose and total acidic HMOs concentrations at 2 M. Infant intakes of 3-fucosyllactose, 3-sialyllactose, 6-sialyllactose, disialyllacto-N-tetraose, disialyllacto-N-hexaose, and total acidic HMOs were positively associated with infant growth over the first 6 M of life.
33572881	2	71	theme	infant	434:439	arg1	growth					441:446	infant growth	434:446	infant growth	434:446	We aimed to quantify the composition of HMOs of women with normal weight (18.5-24.9 kg/m2), overweight (25.0-29.9 kg/m2), or obesity (30.0-60.0 kg/m2) and determine the effect of HMO intake on infant growth.
33572881	5	72	theme	linear	721:726	arg1	models					742:747	linear mixed-effects models	721:747	linear mixed-effects models	721:747	Linear regressions and linear mixed-effects models were conducted examining the relationships between maternal BMI and HMO composition and HMO intake and infant growth over the first 6 M, respectively.
33572881	5	73	theme	Linear	698:703	arg1	regressions					705:715	Linear regressions	698:715	Linear regressions	698:715	Linear regressions and linear mixed-effects models were conducted examining the relationships between maternal BMI and HMO composition and HMO intake and infant growth over the first 6 M, respectively.
33572881	8	74	theme	HMO	1611:1613	arg1	concentrations					1615:1628	HMO concentrations	1611:1628	HMO concentrations that are associated with infant adiposity	1611:1670	Maternal obesity is associated with changes in HMO concentrations that are associated with infant adiposity.
35494573	6	0	theme	glucuronic	735:744	arg1	acid					596:599	galacturonic acid	583:599	galacturonic acid (GalA)	583:606	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	6	0	theme	glucuronic	735:744	arg1	acid					746:749	glucuronic acid	735:749	glucuronic acid	735:749	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	11	1	theme	effective	1541:1549	arg1	activity					1563:1570	the effective coagulation activity	1537:1570	the effective coagulation activity of Leonurus artemisia polysaccharides	1537:1608	These results demonstrated the effective coagulation activity of Leonurus artemisia polysaccharides.
35494573	9	2	theme	SEM	1315:1317	arg1	results					1332:1338	SEM, TEM and AFM results	1315:1338	SEM, TEM and AFM results	1315:1338	SEM, TEM and AFM results indicated that LAP-1 was a highly branched structure.
35494573	7	3	theme	-β-d-GlcAp-	1093:1103	arg1	-β-d-Manp-					1132:1141	→1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→	953:1146	-β-d-Manp-	1132:1141	Primary structure analysis results indicated that LAP-1 contained characteristic glycosyl linkages such as →1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→.
35494573	8	4	theme	LAP-1	1186:1190	arg1	w/M					1155:1157	The M w/M n (PDI), M n, M and M w	1149:1181	The M w/M n (PDI), M n, M and M w of LAP-1	1149:1190	The M w/M n (PDI), M n, M and M w of LAP-1 were determined to be 1.423, 6.979 × 103 g mol-1, 1.409 × 104 g mol-1, and 9.930 × 103 g mol-1 by HPSEC-MALLS-RID and DLS.
35494573	8	4	theme	LAP-1	1186:1190	arg1	1.423					1214:1218	1.423	1214:1218	1.423	1214:1218	The M w/M n (PDI), M n, M and M w of LAP-1 were determined to be 1.423, 6.979 × 103 g mol-1, 1.409 × 104 g mol-1, and 9.930 × 103 g mol-1 by HPSEC-MALLS-RID and DLS.
35494573	9	5	theme	TEM	1320:1322	arg1	results					1332:1338	SEM, TEM and AFM results	1315:1338	SEM, TEM and AFM results	1315:1338	SEM, TEM and AFM results indicated that LAP-1 was a highly branched structure.
35494573	8	6	theme	M	1168:1168	arg1	n					1170:1170	M n	1168:1170	M n	1168:1170	The M w/M n (PDI), M n, M and M w of LAP-1 were determined to be 1.423, 6.979 × 103 g mol-1, 1.409 × 104 g mol-1, and 9.930 × 103 g mol-1 by HPSEC-MALLS-RID and DLS.
35494573	6	7	from	ribose	717:722	arg1	ratio					771:775	the molar ratio	761:775	the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47	761:843	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	7	8	theme	structure	854:862	arg1	results					873:879	Primary structure analysis results	846:879	Primary structure analysis results	846:879	Primary structure analysis results indicated that LAP-1 contained characteristic glycosyl linkages such as →1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→.
35494573	7	9	contain	contained	902:910	arg1	LAP-1					896:900	LAP-1	896:900	LAP-1	896:900	Primary structure analysis results indicated that LAP-1 contained characteristic glycosyl linkages such as →1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→.
35494573	7	9	contain	contained	902:910	arg2	linkages					936:943	characteristic glycosyl linkages	912:943	characteristic glycosyl linkages such as →1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→	912:1146	Primary structure analysis results indicated that LAP-1 contained characteristic glycosyl linkages such as →1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→.
35494573	7	9	contain	contained	902:910	arg2	-β-d-Manp-					1132:1141	→1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→	953:1146	-β-d-Manp-	1132:1141	Primary structure analysis results indicated that LAP-1 contained characteristic glycosyl linkages such as →1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→.
35494573	11	10	theme	Leonurus	1575:1582	arg1	polysaccharides					1594:1608	Leonurus artemisia polysaccharides	1575:1608	Leonurus artemisia polysaccharides	1575:1608	These results demonstrated the effective coagulation activity of Leonurus artemisia polysaccharides.
35494573	4	11	theme	single-factor	378:390	arg1	experiments					392:402	single-factor experiments	378:402	single-factor experiments	378:402	The extraction conditions of the crude polysaccharides were optimized by single-factor experiments and response surface methodology.
35494573	6	12	from	rhamnose	638:645	arg1	ratio					771:775	the molar ratio	761:775	the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47	761:843	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	9	13	theme	AFM	1328:1330	arg1	results					1332:1338	SEM, TEM and AFM results	1315:1338	SEM, TEM and AFM results	1315:1338	SEM, TEM and AFM results indicated that LAP-1 was a highly branched structure.
35494573	7	14	theme	-β-d-GalAp-	1074:1084	arg1	-β-d-Manp-					1132:1141	→1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→	953:1146	-β-d-Manp-	1132:1141	Primary structure analysis results indicated that LAP-1 contained characteristic glycosyl linkages such as →1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→.
35494573	4	15	theme	polysaccharides	344:358	arg1	conditions					320:329	The extraction conditions	305:329	The extraction conditions of the crude polysaccharides	305:358	The extraction conditions of the crude polysaccharides were optimized by single-factor experiments and response surface methodology.
35494573	8	16	theme	×	1227:1227	arg1	mol-1					1235:1239	6.979 × 103 g mol-1	1221:1239	6.979 × 103 g mol-1	1221:1239	The M w/M n (PDI), M n, M and M w of LAP-1 were determined to be 1.423, 6.979 × 103 g mol-1, 1.409 × 104 g mol-1, and 9.930 × 103 g mol-1 by HPSEC-MALLS-RID and DLS.
35494573	6	17	from	mannose	609:615	arg1	ratio					771:775	the molar ratio	761:775	the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47	761:843	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	10	18	theme	anticoagulant	1412:1424	arg1	activity					1426:1433	mild anticoagulant activity	1407:1433	mild anticoagulant activity	1407:1433	LAP-1 showed mild anticoagulant activity, low toxicity, and less spontaneous bleeding compared with heparin sodium.
35494573	7	19	theme	characteristic	912:925	arg1	-β-d-Manp-					1132:1141	→1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→	953:1146	-β-d-Manp-	1132:1141	Primary structure analysis results indicated that LAP-1 contained characteristic glycosyl linkages such as →1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→.
35494573	7	19	theme	characteristic	912:925	arg1	linkages					936:943	characteristic glycosyl linkages	912:943	characteristic glycosyl linkages such as →1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→	912:1146	Primary structure analysis results indicated that LAP-1 contained characteristic glycosyl linkages such as →1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→.
35494573	8	20	theme	g	1233:1233	arg1	mol-1					1235:1239	6.979 × 103 g mol-1	1221:1239	6.979 × 103 g mol-1	1221:1239	The M w/M n (PDI), M n, M and M w of LAP-1 were determined to be 1.423, 6.979 × 103 g mol-1, 1.409 × 104 g mol-1, and 9.930 × 103 g mol-1 by HPSEC-MALLS-RID and DLS.
35494573	3	21	theme	exchange	257:264	arg1	chromatography					266:279	ion exchange chromatography	253:279	ion exchange chromatography	253:279	, and was further purified with ion exchange chromatography and gel chromatography.
35494573	9	22	theme	branched	1374:1381	arg1	structure					1383:1391	a highly branched structure	1365:1391	a highly branched structure	1365:1391	SEM, TEM and AFM results indicated that LAP-1 was a highly branched structure.
35494573	9	22	theme	branched	1374:1381	arg1	LAP-1					1355:1359	LAP-1	1355:1359	LAP-1	1355:1359	SEM, TEM and AFM results indicated that LAP-1 was a highly branched structure.
35494573	5	23	theme	purified	467:474	arg1	polysaccharide					476:489	the purified polysaccharide	463:489	the purified polysaccharide	463:489	The primary structure of the purified polysaccharide was measured by FT-IR, GC-MS, and NMR.
35494573	8	24	theme	×	1248:1248	arg1	mol-1					1256:1260	1.409 × 104 g mol-1	1242:1260	1.409 × 104 g mol-1	1242:1260	The M w/M n (PDI), M n, M and M w of LAP-1 were determined to be 1.423, 6.979 × 103 g mol-1, 1.409 × 104 g mol-1, and 9.930 × 103 g mol-1 by HPSEC-MALLS-RID and DLS.
35494573	6	25	dep	ratio	771:775	arg1	 3.45 					786:791	 3.45 	786:791	 3.45 	786:791	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	6	25	dep	ratio	771:775	arg1	 2.11 					804:809	 2.11 	804:809	 2.11 	804:809	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	6	25	dep	ratio	771:775	arg1	 5.60 					811:816	 5.60 	811:816	 5.60 	811:816	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	6	25	dep	ratio	771:775	arg1	 1.09 					832:837	 1.09 	832:837	 1.09 	832:837	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	6	25	dep	ratio	771:775	arg1	 1.02 					793:798	 1.02 	793:798	 1.02 	793:798	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	6	25	dep	ratio	771:775	arg1	 1.47					839:843	 1.47	839:843	 1.47	839:843	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	6	25	dep	ratio	771:775	arg1	 1 					800:802	 1 	800:802	 1 	800:802	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	6	25	dep	ratio	771:775	arg1	 4.73 					818:823	 4.73 	818:823	 4.73 	818:823	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	6	25	dep	ratio	771:775	arg1	 1.08 					825:830	 1.08 	825:830	 1.08 	825:830	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	7	26	theme	-α-d-Glcp	970:978	arg1	-β-d-GalAp-					1074:1084	-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-	956:1084	-β-d-GalAp-	1074:1084	Primary structure analysis results indicated that LAP-1 contained characteristic glycosyl linkages such as →1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→.
35494573	8	27	theme	g	1254:1254	arg1	mol-1					1256:1260	1.409 × 104 g mol-1	1242:1260	1.409 × 104 g mol-1	1242:1260	The M w/M n (PDI), M n, M and M w of LAP-1 were determined to be 1.423, 6.979 × 103 g mol-1, 1.409 × 104 g mol-1, and 9.930 × 103 g mol-1 by HPSEC-MALLS-RID and DLS.
35494573	12	28	theme	disorders	1722:1730	arg1	treatment					1697:1705	the treatment	1693:1705	the treatment of coagulation disorders	1693:1730	Thus, the purified LAP-1 could be explored as a promising anticoagulant agent for the treatment of coagulation disorders.
35494573	12	29	theme	anticoagulant	1669:1681	arg1	LAP-1					1630:1634	the purified LAP-1	1617:1634	the purified LAP-1	1617:1634	Thus, the purified LAP-1 could be explored as a promising anticoagulant agent for the treatment of coagulation disorders.
35494573	12	29	theme	anticoagulant	1669:1681	arg1	agent					1683:1687	a promising anticoagulant agent	1657:1687	a promising anticoagulant agent for the treatment of coagulation disorders	1657:1730	Thus, the purified LAP-1 could be explored as a promising anticoagulant agent for the treatment of coagulation disorders.
35494573	7	30	theme	-α-d-Manp	956:964	arg1	-β-d-GalAp-					1074:1084	-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-	956:1084	-β-d-GalAp-	1074:1084	Primary structure analysis results indicated that LAP-1 contained characteristic glycosyl linkages such as →1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→.
35494573	6	31	theme	8.74 	780:784	arg1	ratio					771:775	the molar ratio	761:775	the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47	761:843	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	8	32	dep	w/M	1155:1157	arg1	n					1159:1159	n	1159:1159	n (PDI)	1159:1165	The M w/M n (PDI), M n, M and M w of LAP-1 were determined to be 1.423, 6.979 × 103 g mol-1, 1.409 × 104 g mol-1, and 9.930 × 103 g mol-1 by HPSEC-MALLS-RID and DLS.
35494573	8	32	dep	w/M	1155:1157	arg1	M					1173:1173	M	1173:1173	M	1173:1173	The M w/M n (PDI), M n, M and M w of LAP-1 were determined to be 1.423, 6.979 × 103 g mol-1, 1.409 × 104 g mol-1, and 9.930 × 103 g mol-1 by HPSEC-MALLS-RID and DLS.
35494573	8	32	dep	w/M	1155:1157	arg1	n					1170:1170	M n	1168:1170	M n	1168:1170	The M w/M n (PDI), M n, M and M w of LAP-1 were determined to be 1.423, 6.979 × 103 g mol-1, 1.409 × 104 g mol-1, and 9.930 × 103 g mol-1 by HPSEC-MALLS-RID and DLS.
35494573	8	32	dep	w/M	1155:1157	arg1	PDI					1162:1164	PDI	1162:1164	PDI	1162:1164	The M w/M n (PDI), M n, M and M w of LAP-1 were determined to be 1.423, 6.979 × 103 g mol-1, 1.409 × 104 g mol-1, and 9.930 × 103 g mol-1 by HPSEC-MALLS-RID and DLS.
35494573	8	32	dep	w/M	1155:1157	arg1	M					1179:1179	M	1179:1179	M	1179:1179	The M w/M n (PDI), M n, M and M w of LAP-1 were determined to be 1.423, 6.979 × 103 g mol-1, 1.409 × 104 g mol-1, and 9.930 × 103 g mol-1 by HPSEC-MALLS-RID and DLS.
35494573	2	33	attach	isolated	181:188	arg2	polysaccharide					134:147	An acidic polysaccharide	124:147	An acidic polysaccharide	124:147	An acidic polysaccharide, named LAP-1, was extracted and isolated from Leonurus artemisia (Laur.)
35494573	2	33	attach	isolated	181:188	arg1	artemisia					204:212	Leonurus artemisia	195:212	Leonurus artemisia	195:212	An acidic polysaccharide, named LAP-1, was extracted and isolated from Leonurus artemisia (Laur.)
35494573	0	34	from	activity	45:52	arg1	artemisia					94:102	Leonurus artemisia	85:102	Leonurus artemisia	85:102	Structure characterization and anticoagulant activity of a novel polysaccharide from Leonurus artemisia (Laur.)
35494573	0	35	theme	Structure	0:8	arg1	Laur					105:108	Laur	105:108	Laur	105:108	Structure characterization and anticoagulant activity of a novel polysaccharide from Leonurus artemisia (Laur.)
35494573	0	35	theme	Structure	0:8	arg1	characterization					10:25	Structure characterization	0:25	Structure characterization	0:25	Structure characterization and anticoagulant activity of a novel polysaccharide from Leonurus artemisia (Laur.)
35494573	4	36	theme	surface	417:423	arg1	methodology					425:435	response surface methodology	408:435	response surface methodology	408:435	The extraction conditions of the crude polysaccharides were optimized by single-factor experiments and response surface methodology.
35494573	6	37	from	galactose	686:694	arg1	ratio					771:775	the molar ratio	761:775	the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47	761:843	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	11	38	theme	polysaccharides	1594:1608	arg1	activity					1563:1570	the effective coagulation activity	1537:1570	the effective coagulation activity of Leonurus artemisia polysaccharides	1537:1608	These results demonstrated the effective coagulation activity of Leonurus artemisia polysaccharides.
35494573	7	39	theme	glycosyl	927:934	arg1	-β-d-Manp-					1132:1141	→1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→	953:1146	-β-d-Manp-	1132:1141	Primary structure analysis results indicated that LAP-1 contained characteristic glycosyl linkages such as →1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→.
35494573	7	39	theme	glycosyl	927:934	arg1	linkages					936:943	characteristic glycosyl linkages	912:943	characteristic glycosyl linkages such as →1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→	912:1146	Primary structure analysis results indicated that LAP-1 contained characteristic glycosyl linkages such as →1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→.
35494573	12	40	theme	purified	1621:1628	arg1	agent					1683:1687	a promising anticoagulant agent	1657:1687	a promising anticoagulant agent for the treatment of coagulation disorders	1657:1730	Thus, the purified LAP-1 could be explored as a promising anticoagulant agent for the treatment of coagulation disorders.
35494573	12	40	theme	purified	1621:1628	arg1	LAP-1					1630:1634	the purified LAP-1	1617:1634	the purified LAP-1	1617:1634	Thus, the purified LAP-1 could be explored as a promising anticoagulant agent for the treatment of coagulation disorders.
35494573	4	41	theme	extraction	309:318	arg1	conditions					320:329	The extraction conditions	305:329	The extraction conditions of the crude polysaccharides	305:358	The extraction conditions of the crude polysaccharides were optimized by single-factor experiments and response surface methodology.
35494573	7	42	theme	-β-d-Galp-	1002:1011	arg1	-β-d-GalAp-					1074:1084	-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-	956:1084	-β-d-GalAp-	1074:1084	Primary structure analysis results indicated that LAP-1 contained characteristic glycosyl linkages such as →1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→.
35494573	0	43	theme	polysaccharide	65:78	arg1	Laur					105:108	Laur	105:108	Laur	105:108	Structure characterization and anticoagulant activity of a novel polysaccharide from Leonurus artemisia (Laur.)
35494573	0	43	theme	polysaccharide	65:78	arg1	characterization					10:25	Structure characterization	0:25	Structure characterization	0:25	Structure characterization and anticoagulant activity of a novel polysaccharide from Leonurus artemisia (Laur.)
35494573	0	43	theme	polysaccharide	65:78	arg1	activity					45:52	anticoagulant activity	31:52	anticoagulant activity	31:52	Structure characterization and anticoagulant activity of a novel polysaccharide from Leonurus artemisia (Laur.)
35494573	6	44	from	arabinose	654:662	arg1	ratio					771:775	the molar ratio	761:775	the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47	761:843	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	10	45	theme	heparin	1494:1500	arg1	sodium					1502:1507	heparin sodium	1494:1507	heparin sodium	1494:1507	LAP-1 showed mild anticoagulant activity, low toxicity, and less spontaneous bleeding compared with heparin sodium.
35494573	8	46	theme	M	1153:1153	arg1	w/M					1155:1157	The M w/M n (PDI), M n, M and M w	1149:1181	The M w/M n (PDI), M n, M and M w of LAP-1	1149:1190	The M w/M n (PDI), M n, M and M w of LAP-1 were determined to be 1.423, 6.979 × 103 g mol-1, 1.409 × 104 g mol-1, and 9.930 × 103 g mol-1 by HPSEC-MALLS-RID and DLS.
35494573	8	46	theme	M	1153:1153	arg1	1.423					1214:1218	1.423	1214:1218	1.423	1214:1218	The M w/M n (PDI), M n, M and M w of LAP-1 were determined to be 1.423, 6.979 × 103 g mol-1, 1.409 × 104 g mol-1, and 9.930 × 103 g mol-1 by HPSEC-MALLS-RID and DLS.
35494573	7	47	theme	-α-d-Arap-	984:993	arg1	-β-d-GalAp-					1074:1084	-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-	956:1084	-β-d-GalAp-	1074:1084	Primary structure analysis results indicated that LAP-1 contained characteristic glycosyl linkages such as →1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→.
35494573	6	48	from	acid	746:749	arg1	ratio					771:775	the molar ratio	761:775	the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47	761:843	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	7	49	theme	analysis	864:871	arg1	results					873:879	Primary structure analysis results	846:879	Primary structure analysis results	846:879	Primary structure analysis results indicated that LAP-1 contained characteristic glycosyl linkages such as →1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→.
35494573	11	50	theme	coagulation	1551:1561	arg1	activity					1563:1570	the effective coagulation activity	1537:1570	the effective coagulation activity of Leonurus artemisia polysaccharides	1537:1608	These results demonstrated the effective coagulation activity of Leonurus artemisia polysaccharides.
35494573	7	51	theme	Primary	846:852	arg1	results					873:879	Primary structure analysis results	846:879	Primary structure analysis results	846:879	Primary structure analysis results indicated that LAP-1 contained characteristic glycosyl linkages such as →1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→.
35494573	2	52	theme	acidic	127:132	arg1	polysaccharide					134:147	An acidic polysaccharide	124:147	An acidic polysaccharide	124:147	An acidic polysaccharide, named LAP-1, was extracted and isolated from Leonurus artemisia (Laur.)
35494573	11	53	theme	artemisia	1584:1592	arg1	polysaccharides					1594:1608	Leonurus artemisia polysaccharides	1575:1608	Leonurus artemisia polysaccharides	1575:1608	These results demonstrated the effective coagulation activity of Leonurus artemisia polysaccharides.
35494573	4	54	theme	crude	338:342	arg1	polysaccharides					344:358	the crude polysaccharides	334:358	the crude polysaccharides	334:358	The extraction conditions of the crude polysaccharides were optimized by single-factor experiments and response surface methodology.
35494573	6	55	theme	galacturonic	583:594	arg1	acid					596:599	galacturonic acid	583:599	galacturonic acid (GalA)	583:606	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	6	55	theme	galacturonic	583:594	arg1	fucose					703:708	fucose	703:708	fucose (Fuc)	703:714	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	6	55	theme	galacturonic	583:594	arg1	arabinose					654:662	arabinose	654:662	arabinose	654:662	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	6	55	theme	galacturonic	583:594	arg1	rhamnose					638:645	rhamnose	638:645	rhamnose (Rha)	638:651	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	6	55	theme	galacturonic	583:594	arg1	GalA					602:605	GalA	602:605	GalA	602:605	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	6	55	theme	galacturonic	583:594	arg1	ribose					717:722	ribose	717:722	ribose (Rib)	717:728	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	6	55	theme	galacturonic	583:594	arg1	acid					746:749	glucuronic acid	735:749	glucuronic acid	735:749	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	6	55	theme	galacturonic	583:594	arg1	xylose					624:629	xylose	624:629	xylose (Xyl)	624:635	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	6	55	theme	galacturonic	583:594	arg1	galactose					686:694	galactose	686:694	galactose	686:694	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	6	55	theme	galacturonic	583:594	arg1	mannose					609:615	mannose	609:615	mannose (Man)	609:621	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	6	55	theme	galacturonic	583:594	arg1	glucose					671:677	glucose	671:677	glucose	671:677	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	7	56	theme	-β-d-Glcp-	1056:1065	arg1	-β-d-GalAp-					1074:1084	-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-	956:1084	-β-d-GalAp-	1074:1084	Primary structure analysis results indicated that LAP-1 contained characteristic glycosyl linkages such as →1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→.
35494573	6	57	theme	molar	765:769	arg1	ratio					771:775	the molar ratio	761:775	the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47	761:843	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	0	58	from	artemisia	94:102	arg1	Laur					105:108	Laur	105:108	Laur	105:108	Structure characterization and anticoagulant activity of a novel polysaccharide from Leonurus artemisia (Laur.)
35494573	0	58	from	artemisia	94:102	arg1	polysaccharide					65:78	a novel polysaccharide	57:78	a novel polysaccharide from Leonurus artemisia	57:102	Structure characterization and anticoagulant activity of a novel polysaccharide from Leonurus artemisia (Laur.)
35494573	0	58	from	artemisia	94:102	arg1	characterization					10:25	Structure characterization	0:25	Structure characterization	0:25	Structure characterization and anticoagulant activity of a novel polysaccharide from Leonurus artemisia (Laur.)
35494573	0	58	from	artemisia	94:102	arg1	activity					45:52	anticoagulant activity	31:52	anticoagulant activity	31:52	Structure characterization and anticoagulant activity of a novel polysaccharide from Leonurus artemisia (Laur.)
35494573	6	59	from	xylose	624:629	arg1	ratio					771:775	the molar ratio	761:775	the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47	761:843	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	5	60	theme	polysaccharide	476:489	arg1	structure					450:458	The primary structure	438:458	The primary structure of the purified polysaccharide	438:489	The primary structure of the purified polysaccharide was measured by FT-IR, GC-MS, and NMR.
35494573	6	61	from	fucose	703:708	arg1	ratio					771:775	the molar ratio	761:775	the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47	761:843	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	10	62	theme	mild	1407:1410	arg1	activity					1426:1433	mild anticoagulant activity	1407:1433	mild anticoagulant activity	1407:1433	LAP-1 showed mild anticoagulant activity, low toxicity, and less spontaneous bleeding compared with heparin sodium.
35494573	2	63	theme	Leonurus	195:202	arg1	artemisia					204:212	Leonurus artemisia	195:212	Leonurus artemisia	195:212	An acidic polysaccharide, named LAP-1, was extracted and isolated from Leonurus artemisia (Laur.)
35494573	4	64	theme	response	408:415	arg1	methodology					425:435	response surface methodology	408:435	response surface methodology	408:435	The extraction conditions of the crude polysaccharides were optimized by single-factor experiments and response surface methodology.
35494573	7	65	theme	-β-d-Galp-	1038:1047	arg1	-β-d-GalAp-					1074:1084	-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-	956:1084	-β-d-GalAp-	1074:1084	Primary structure analysis results indicated that LAP-1 contained characteristic glycosyl linkages such as →1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→.
35494573	1	66	theme	Hu	118:119	arg1	F					121:121	Hu F	118:121	S. Y. Hu F.	112:122	S. Y. Hu F.
35494573	3	67	theme	ion	253:255	arg1	chromatography					266:279	ion exchange chromatography	253:279	ion exchange chromatography	253:279	, and was further purified with ion exchange chromatography and gel chromatography.
35494573	10	68	theme	low	1436:1438	arg1	toxicity					1440:1447	low toxicity	1436:1447	low toxicity	1436:1447	LAP-1 showed mild anticoagulant activity, low toxicity, and less spontaneous bleeding compared with heparin sodium.
35494573	7	69	theme	4→	1031:1032	arg1	-β-d-GalAp-					1074:1084	-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-	956:1084	-β-d-GalAp-	1074:1084	Primary structure analysis results indicated that LAP-1 contained characteristic glycosyl linkages such as →1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→.
35494573	3	70	theme	gel	285:287	arg1	chromatography					289:302	gel chromatography	285:302	gel chromatography	285:302	, and was further purified with ion exchange chromatography and gel chromatography.
35494573	0	71	from	characterization	10:25	arg1	artemisia					94:102	Leonurus artemisia	85:102	Leonurus artemisia	85:102	Structure characterization and anticoagulant activity of a novel polysaccharide from Leonurus artemisia (Laur.)
35494573	12	72	theme	promising	1659:1667	arg1	LAP-1					1630:1634	the purified LAP-1	1617:1634	the purified LAP-1	1617:1634	Thus, the purified LAP-1 could be explored as a promising anticoagulant agent for the treatment of coagulation disorders.
35494573	12	72	theme	promising	1659:1667	arg1	agent					1683:1687	a promising anticoagulant agent	1657:1687	a promising anticoagulant agent for the treatment of coagulation disorders	1657:1730	Thus, the purified LAP-1 could be explored as a promising anticoagulant agent for the treatment of coagulation disorders.
35494573	8	73	dep	n	1159:1159	arg1	w					1181:1181	w	1181:1181	w	1181:1181	The M w/M n (PDI), M n, M and M w of LAP-1 were determined to be 1.423, 6.979 × 103 g mol-1, 1.409 × 104 g mol-1, and 9.930 × 103 g mol-1 by HPSEC-MALLS-RID and DLS.
35494573	12	74	theme	coagulation	1710:1720	arg1	disorders					1722:1730	coagulation disorders	1710:1730	coagulation disorders	1710:1730	Thus, the purified LAP-1 could be explored as a promising anticoagulant agent for the treatment of coagulation disorders.
35494573	1	75	dep	Y.	115:116	arg1	F					121:121	Hu F	118:121	S. Y. Hu F.	112:122	S. Y. Hu F.
35494573	8	76	theme	×	1273:1273	arg1	mol-1					1281:1285	9.930 × 103 g mol-1	1267:1285	9.930 × 103 g mol-1	1267:1285	The M w/M n (PDI), M n, M and M w of LAP-1 were determined to be 1.423, 6.979 × 103 g mol-1, 1.409 × 104 g mol-1, and 9.930 × 103 g mol-1 by HPSEC-MALLS-RID and DLS.
35494573	0	77	theme	anticoagulant	31:43	arg1	activity					45:52	anticoagulant activity	31:52	anticoagulant activity	31:52	Structure characterization and anticoagulant activity of a novel polysaccharide from Leonurus artemisia (Laur.)
35494573	8	78	theme	g	1279:1279	arg1	mol-1					1281:1285	9.930 × 103 g mol-1	1267:1285	9.930 × 103 g mol-1	1267:1285	The M w/M n (PDI), M n, M and M w of LAP-1 were determined to be 1.423, 6.979 × 103 g mol-1, 1.409 × 104 g mol-1, and 9.930 × 103 g mol-1 by HPSEC-MALLS-RID and DLS.
35494573	6	79	from	glucose	671:677	arg1	ratio					771:775	the molar ratio	761:775	the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47	761:843	The results showed that LAP-1 was mainly composed of galacturonic acid (GalA), mannose (Man), xylose (Xyl), rhamnose (Rha), arabinose (Ara), glucose (Glc), galactose (Gal), fucose (Fuc), ribose (Rib), and glucuronic acid (GlcA) in the molar ratio of 8.74 : 3.45 : 1.02 : 1 : 2.11 : 5.60 : 4.73 : 1.08 : 1.09 : 1.47.
35494573	0	80	theme	novel	59:63	arg1	polysaccharide					65:78	a novel polysaccharide	57:78	a novel polysaccharide from Leonurus artemisia	57:102	Structure characterization and anticoagulant activity of a novel polysaccharide from Leonurus artemisia (Laur.)
35494573	7	81	dep	-β-d-GalAp-	1074:1084	arg1	→1					967:968	-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-	956:1084	→1	967:968	Primary structure analysis results indicated that LAP-1 contained characteristic glycosyl linkages such as →1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→.
35494573	7	81	dep	-β-d-GalAp-	1074:1084	arg1	→1					1035:1036	-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-	956:1084	→1	1035:1036	Primary structure analysis results indicated that LAP-1 contained characteristic glycosyl linkages such as →1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→.
35494573	7	81	dep	-β-d-GalAp-	1074:1084	arg1	→1					981:982	-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-	956:1084	→1	981:982	Primary structure analysis results indicated that LAP-1 contained characteristic glycosyl linkages such as →1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→.
35494573	7	82	theme	-β-d-Manp-	1112:1121	arg1	-β-d-Manp-					1132:1141	→1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→	953:1146	-β-d-Manp-	1132:1141	Primary structure analysis results indicated that LAP-1 contained characteristic glycosyl linkages such as →1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→.
35494573	2	83	dep	extracted	167:175	arg1	Laur					215:218	Laur	215:218	Laur	215:218	An acidic polysaccharide, named LAP-1, was extracted and isolated from Leonurus artemisia (Laur.)
35494573	5	84	theme	primary	442:448	arg1	structure					450:458	The primary structure	438:458	The primary structure of the purified polysaccharide	438:489	The primary structure of the purified polysaccharide was measured by FT-IR, GC-MS, and NMR.
35494573	7	85	theme	-β-d-Manp-	1020:1029	arg1	-β-d-GalAp-					1074:1084	-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-	956:1084	-β-d-GalAp-	1074:1084	Primary structure analysis results indicated that LAP-1 contained characteristic glycosyl linkages such as →1)-α-d-Manp, →1)-α-d-Glcp, →1)-α-d-Arap-(2→, →1)-β-d-Galp-(3→, →1)-β-d-Manp-(4→, →1)-β-d-Galp-(4→, →1)-β-d-Glcp-(4→, →1)-β-d-GalAp-(4→, →1)-β-d-GlcAp-(4→, →1)-β-d-Manp-(4,6→, →1)-β-d-Manp-(3,4→.
32600722	3	0	theme	DS-loaded	633:641	arg1	p					643:643	DS-loaded p	633:643	DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C]	633:726	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	0	theme	DS-loaded	633:641	arg1	phase					751:755	the dispersed phase	737:755	the dispersed phase	737:755	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	0	theme	DS-loaded	633:641	arg1	nanogels					593:600	thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels	537:600	thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C)	537:627	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	2	1	theme	basic	328:332	arg1	bFGF					360:363	bFGF	360:363	bFGF	360:363	In this study, we developed a composite hydrogel dressing with stepwise delivery of diclofenac sodium (DS) and basic fibroblast growth factor (bFGF) in the inflammation stage and new tissue formation stage respectively for wound repair.
32600722	2	1	theme	basic	328:332	arg1	factor					352:357	basic fibroblast growth factor	328:357	basic fibroblast growth factor (bFGF)	328:364	In this study, we developed a composite hydrogel dressing with stepwise delivery of diclofenac sodium (DS) and basic fibroblast growth factor (bFGF) in the inflammation stage and new tissue formation stage respectively for wound repair.
32600722	3	2	theme	nanogels	684:691	arg1	NGs					709:711	N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs	645:711	N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs	645:711	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	2	theme	nanogels	684:691	arg1	~40 °C					720:725	LCST2 ~40 °C	714:725	LCST2 ~40 °C	714:725	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	4	3	theme	@	781:781	arg1	hydrogel					807:814	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel	758:814	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel	758:814	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	3	4	theme	acid	678:681	arg1	NGs					709:711	N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs	645:711	N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs	645:711	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	4	theme	acid	678:681	arg1	~40 °C					720:725	LCST2 ~40 °C	714:725	LCST2 ~40 °C	714:725	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	5	5	theme	new	1291:1293	arg1	formation					1302:1310	new tissue formation stage	1291:1316	new tissue formation stage	1291:1316	The in vitro thermosensitive cargo delivery of this hydrogel showed that 92% of DS was sustainably delivered at 37 °C within the early three days mimicking the inflammation stage, while 80% of bFGF was controlled released at 25 °C within the later eight days mimicking new tissue formation stage.
32600722	2	6	theme	hydrogel	257:264	arg1	dressing					266:273	a composite hydrogel dressing	245:273	a composite hydrogel dressing with stepwise delivery of diclofenac sodium (DS) and basic fibroblast growth factor (bFGF)	245:364	In this study, we developed a composite hydrogel dressing with stepwise delivery of diclofenac sodium (DS) and basic fibroblast growth factor (bFGF) in the inflammation stage and new tissue formation stage respectively for wound repair.
32600722	4	7	theme	@	791:791	arg1	hydrogel					807:814	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel	758:814	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel	758:814	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	7	8	theme	potential	1613:1621	arg1	hydrogel					1599:1606	SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel	1556:1606	SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel	1556:1606	These findings indicate SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel is a potential dressing for wound repair.
32600722	7	8	theme	potential	1613:1621	arg1	dressing					1623:1630	a potential dressing	1611:1630	a potential dressing for wound repair	1611:1647	These findings indicate SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel is a potential dressing for wound repair.
32600722	2	9	theme	diclofenac	301:310	arg1	DS					320:321	DS	320:321	DS	320:321	In this study, we developed a composite hydrogel dressing with stepwise delivery of diclofenac sodium (DS) and basic fibroblast growth factor (bFGF) in the inflammation stage and new tissue formation stage respectively for wound repair.
32600722	2	9	theme	diclofenac	301:310	arg1	sodium					312:317	diclofenac sodium	301:317	diclofenac sodium (DS)	301:322	In this study, we developed a composite hydrogel dressing with stepwise delivery of diclofenac sodium (DS) and basic fibroblast growth factor (bFGF) in the inflammation stage and new tissue formation stage respectively for wound repair.
32600722	6	10	theme	better	1401:1406	arg1	effect					1416:1421	a better healing effect	1399:1421	a better healing effect	1399:1421	The in vivo wound healing of rats showed that this composite hydrogel presented a better healing effect with a wound contraction of 96% at 14 d, less inflammation and higher angiogenesis, than all control groups.
32600722	0	11	theme	factor	116:121	arg1	delivery					88:95	stepwise delivery	79:95	stepwise delivery of drug and growth factor for wound repair	79:138	An alginate/poly(N-isopropylacrylamide)-based composite hydrogel dressing with stepwise delivery of drug and growth factor for wound repair.
32600722	3	12	theme	continuous	515:524	arg1	alginate					461:468	Sodium alginate	454:468	Sodium alginate (SA) crosslinked by calcium ion	454:500	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	12	theme	continuous	515:524	arg1	phase					526:530	the continuous phase	511:530	the continuous phase	511:530	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	1	13	theme	wound	202:206	arg1	healing					208:214	wound healing	202:214	wound healing	202:214	Anti-inflammation and angiogenesis play an essential role in wound healing.
32600722	4	14	theme	~4500 Pa	857:864	arg1	modulus					846:852	a desirable storage modulus	826:852	a desirable storage modulus of ~4500 Pa	826:864	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	4	14	theme	~4500 Pa	857:864	arg1	ratio					901:905	a high water equilibrium swelling ratio	867:905	a high water equilibrium swelling ratio of ~90	867:912	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	4	14	theme	~4500 Pa	857:864	arg1	nontoxicity					983:993	nontoxicity	983:993	nontoxicity to human skin fibroblasts	983:1019	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	4	14	theme	~4500 Pa	857:864	arg1	rate					955:958	an appropriate water vapor transmission rate	915:958	an appropriate water vapor transmission rate of ~2300 g/m2/day	915:976	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	0	15	theme	wound	127:131	arg1	repair					133:138	wound repair	127:138	wound repair	127:138	An alginate/poly(N-isopropylacrylamide)-based composite hydrogel dressing with stepwise delivery of drug and growth factor for wound repair.
32600722	3	16	theme	poly	565:568	arg1	p					643:643	DS-loaded p	633:643	DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C]	633:726	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	16	theme	poly	565:568	arg1	phase					751:755	the dispersed phase	737:755	the dispersed phase	737:755	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	16	theme	poly	565:568	arg1	nanogels					593:600	thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels	537:600	thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C)	537:627	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	17	dep	nanogels	593:600	arg1	NGs					610:612	pNIPAM NGs	603:612	pNIPAM NGs	603:612	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	17	dep	nanogels	593:600	arg1	~33 °C					621:626	LCST1 ~33 °C	615:626	LCST1 ~33 °C	615:626	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	18	theme	thermosensitive	537:551	arg1	p					643:643	DS-loaded p	633:643	DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C]	633:726	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	18	theme	thermosensitive	537:551	arg1	phase					751:755	the dispersed phase	737:755	the dispersed phase	737:755	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	18	theme	thermosensitive	537:551	arg1	nanogels					593:600	thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels	537:600	thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C)	537:627	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	4	19	theme	desirable	828:836	arg1	modulus					846:852	a desirable storage modulus	826:852	a desirable storage modulus of ~4500 Pa	826:864	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	5	20	theme	inflammation	1182:1193	arg1	stage					1195:1199	the inflammation stage	1178:1199	the inflammation stage	1178:1199	The in vitro thermosensitive cargo delivery of this hydrogel showed that 92% of DS was sustainably delivered at 37 °C within the early three days mimicking the inflammation stage, while 80% of bFGF was controlled released at 25 °C within the later eight days mimicking new tissue formation stage.
32600722	4	21	theme	swelling	892:899	arg1	ratio					901:905	a high water equilibrium swelling ratio	867:905	a high water equilibrium swelling ratio of ~90	867:912	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	4	22	theme	transmission	942:953	arg1	rate					955:958	an appropriate water vapor transmission rate	915:958	an appropriate water vapor transmission rate of ~2300 g/m2/day	915:976	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	5	23	dep	formation	1302:1310	arg1	stage					1312:1316	stage	1312:1316	new tissue formation stage	1291:1316	The in vitro thermosensitive cargo delivery of this hydrogel showed that 92% of DS was sustainably delivered at 37 °C within the early three days mimicking the inflammation stage, while 80% of bFGF was controlled released at 25 °C within the later eight days mimicking new tissue formation stage.
32600722	0	24	theme	stepwise	79:86	arg1	delivery					88:95	stepwise delivery	79:95	stepwise delivery of drug and growth factor for wound repair	79:138	An alginate/poly(N-isopropylacrylamide)-based composite hydrogel dressing with stepwise delivery of drug and growth factor for wound repair.
32600722	5	25	theme	cargo	1051:1055	arg1	delivery					1057:1064	The in vitro thermosensitive cargo delivery	1022:1064	The in vitro thermosensitive cargo delivery of this hydrogel	1022:1081	The in vitro thermosensitive cargo delivery of this hydrogel showed that 92% of DS was sustainably delivered at 37 °C within the early three days mimicking the inflammation stage, while 80% of bFGF was controlled released at 25 °C within the later eight days mimicking new tissue formation stage.
32600722	7	26	theme	@	1563:1563	arg1	hydrogel					1599:1606	SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel	1556:1606	SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel	1556:1606	These findings indicate SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel is a potential dressing for wound repair.
32600722	7	26	theme	@	1563:1563	arg1	dressing					1623:1630	a potential dressing	1611:1630	a potential dressing for wound repair	1611:1647	These findings indicate SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel is a potential dressing for wound repair.
32600722	4	27	theme	water	874:878	arg1	ratio					901:905	a high water equilibrium swelling ratio	867:905	a high water equilibrium swelling ratio of ~90	867:912	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	4	28	theme	vapor	936:940	arg1	rate					955:958	an appropriate water vapor transmission rate	915:958	an appropriate water vapor transmission rate of ~2300 g/m2/day	915:976	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	6	29	theme	in	1323:1324	arg1	healing					1337:1343	The in vivo wound healing	1319:1343	The in vivo wound healing of rats	1319:1351	The in vivo wound healing of rats showed that this composite hydrogel presented a better healing effect with a wound contraction of 96% at 14 d, less inflammation and higher angiogenesis, than all control groups.
32600722	6	30	theme	less	1464:1467	arg1	inflammation					1469:1480	less inflammation	1464:1480	less inflammation	1464:1480	The in vivo wound healing of rats showed that this composite hydrogel presented a better healing effect with a wound contraction of 96% at 14 d, less inflammation and higher angiogenesis, than all control groups.
32600722	2	31	theme	tissue	400:405	arg1	formation					407:415	new tissue formation	396:415	new tissue formation	396:415	In this study, we developed a composite hydrogel dressing with stepwise delivery of diclofenac sodium (DS) and basic fibroblast growth factor (bFGF) in the inflammation stage and new tissue formation stage respectively for wound repair.
32600722	2	32	theme	inflammation	373:384	arg1	stage					386:390	inflammation stage	373:390	inflammation stage	373:390	In this study, we developed a composite hydrogel dressing with stepwise delivery of diclofenac sodium (DS) and basic fibroblast growth factor (bFGF) in the inflammation stage and new tissue formation stage respectively for wound repair.
32600722	2	33	theme	wound	440:444	arg1	repair					446:451	wound repair	440:451	wound repair	440:451	In this study, we developed a composite hydrogel dressing with stepwise delivery of diclofenac sodium (DS) and basic fibroblast growth factor (bFGF) in the inflammation stage and new tissue formation stage respectively for wound repair.
32600722	5	34	dep	controlled	1224:1233	arg1	released					1235:1242	released	1235:1242	was controlled released at 25 °C within the later eight days mimicking new tissue formation stage	1220:1316	The in vitro thermosensitive cargo delivery of this hydrogel showed that 92% of DS was sustainably delivered at 37 °C within the early three days mimicking the inflammation stage, while 80% of bFGF was controlled released at 25 °C within the later eight days mimicking new tissue formation stage.
32600722	4	35	theme	appropriate	918:928	arg1	rate					955:958	an appropriate water vapor transmission rate	915:958	an appropriate water vapor transmission rate of ~2300 g/m2/day	915:976	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	5	36	dep	in	1026:1027	arg1	vitro					1029:1033	vitro	1029:1033	vitro	1029:1033	The in vitro thermosensitive cargo delivery of this hydrogel showed that 92% of DS was sustainably delivered at 37 °C within the early three days mimicking the inflammation stage, while 80% of bFGF was controlled released at 25 °C within the later eight days mimicking new tissue formation stage.
32600722	3	37	theme	pNIPAM	603:608	arg1	NGs					610:612	pNIPAM NGs	603:612	pNIPAM NGs	603:612	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	37	theme	pNIPAM	603:608	arg1	~33 °C					621:626	LCST1 ~33 °C	615:626	LCST1 ~33 °C	615:626	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	4	38	theme	synthesized	762:772	arg1	hydrogel					807:814	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel	758:814	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel	758:814	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	0	39	theme	composite	46:54	arg1	dressing					65:72	An alginate/poly(N-isopropylacrylamide)-based composite hydrogel dressing	0:72	An alginate/poly(N-isopropylacrylamide)-based composite hydrogel dressing with stepwise delivery of drug and growth factor for wound repair.	0:139	An alginate/poly(N-isopropylacrylamide)-based composite hydrogel dressing with stepwise delivery of drug and growth factor for wound repair.
32600722	5	40	theme	hydrogel	1074:1081	arg1	delivery					1057:1064	The in vitro thermosensitive cargo delivery	1022:1064	The in vitro thermosensitive cargo delivery of this hydrogel	1022:1081	The in vitro thermosensitive cargo delivery of this hydrogel showed that 92% of DS was sustainably delivered at 37 °C within the early three days mimicking the inflammation stage, while 80% of bFGF was controlled released at 25 °C within the later eight days mimicking new tissue formation stage.
32600722	3	41	theme	N-isopropylacrylamide	570:590	arg1	p					643:643	DS-loaded p	633:643	DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C]	633:726	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	41	theme	N-isopropylacrylamide	570:590	arg1	phase					751:755	the dispersed phase	737:755	the dispersed phase	737:755	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	41	theme	N-isopropylacrylamide	570:590	arg1	nanogels					593:600	thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels	537:600	thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C)	537:627	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	6	42	dep	in	1323:1324	arg1	vivo					1326:1329	vivo	1326:1329	vivo	1326:1329	The in vivo wound healing of rats showed that this composite hydrogel presented a better healing effect with a wound contraction of 96% at 14 d, less inflammation and higher angiogenesis, than all control groups.
32600722	4	43	theme	skin	1004:1007	arg1	fibroblasts					1009:1019	human skin fibroblasts	998:1019	human skin fibroblasts	998:1019	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	7	44	theme	@	1573:1573	arg1	hydrogel					1599:1606	SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel	1556:1606	SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel	1556:1606	These findings indicate SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel is a potential dressing for wound repair.
32600722	7	44	theme	@	1573:1573	arg1	dressing					1623:1630	a potential dressing	1611:1630	a potential dressing for wound repair	1611:1647	These findings indicate SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel is a potential dressing for wound repair.
32600722	2	45	theme	factor	352:357	arg1	delivery					289:296	stepwise delivery	280:296	stepwise delivery of diclofenac sodium (DS) and basic fibroblast growth factor (bFGF)	280:364	In this study, we developed a composite hydrogel dressing with stepwise delivery of diclofenac sodium (DS) and basic fibroblast growth factor (bFGF) in the inflammation stage and new tissue formation stage respectively for wound repair.
32600722	4	46	theme	~2300 g/m2/day	963:976	arg1	modulus					846:852	a desirable storage modulus	826:852	a desirable storage modulus of ~4500 Pa	826:864	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	4	46	theme	~2300 g/m2/day	963:976	arg1	ratio					901:905	a high water equilibrium swelling ratio	867:905	a high water equilibrium swelling ratio of ~90	867:912	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	4	46	theme	~2300 g/m2/day	963:976	arg1	nontoxicity					983:993	nontoxicity	983:993	nontoxicity to human skin fibroblasts	983:1019	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	4	46	theme	~2300 g/m2/day	963:976	arg1	rate					955:958	an appropriate water vapor transmission rate	915:958	an appropriate water vapor transmission rate of ~2300 g/m2/day	915:976	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	3	47	theme	N-isopropylacrylamide-co-acrylic	645:676	arg1	NGs					709:711	N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs	645:711	N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs	645:711	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	47	theme	N-isopropylacrylamide-co-acrylic	645:676	arg1	~40 °C					720:725	LCST2 ~40 °C	714:725	LCST2 ~40 °C	714:725	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	2	48	theme	fibroblast	334:343	arg1	bFGF					360:363	bFGF	360:363	bFGF	360:363	In this study, we developed a composite hydrogel dressing with stepwise delivery of diclofenac sodium (DS) and basic fibroblast growth factor (bFGF) in the inflammation stage and new tissue formation stage respectively for wound repair.
32600722	2	48	theme	fibroblast	334:343	arg1	factor					352:357	basic fibroblast growth factor	328:357	basic fibroblast growth factor (bFGF)	328:364	In this study, we developed a composite hydrogel dressing with stepwise delivery of diclofenac sodium (DS) and basic fibroblast growth factor (bFGF) in the inflammation stage and new tissue formation stage respectively for wound repair.
32600722	5	49	theme	DS	1102:1103	arg1	DS					1102:1103	DS	1102:1103	DS	1102:1103	The in vitro thermosensitive cargo delivery of this hydrogel showed that 92% of DS was sustainably delivered at 37 °C within the early three days mimicking the inflammation stage, while 80% of bFGF was controlled released at 25 °C within the later eight days mimicking new tissue formation stage.
32600722	5	49	theme	DS	1102:1103	arg1	%					1097:1097	92%	1095:1097	92% of DS	1095:1103	The in vitro thermosensitive cargo delivery of this hydrogel showed that 92% of DS was sustainably delivered at 37 °C within the early three days mimicking the inflammation stage, while 80% of bFGF was controlled released at 25 °C within the later eight days mimicking new tissue formation stage.
32600722	4	50	theme	p	792:792	arg1	hydrogel					807:814	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel	758:814	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel	758:814	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	7	51	theme	wound	1636:1640	arg1	repair					1642:1647	wound repair	1636:1647	wound repair	1636:1647	These findings indicate SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel is a potential dressing for wound repair.
32600722	2	52	theme	stepwise	280:287	arg1	delivery					289:296	stepwise delivery	280:296	stepwise delivery of diclofenac sodium (DS) and basic fibroblast growth factor (bFGF)	280:364	In this study, we developed a composite hydrogel dressing with stepwise delivery of diclofenac sodium (DS) and basic fibroblast growth factor (bFGF) in the inflammation stage and new tissue formation stage respectively for wound repair.
32600722	6	53	theme	wound	1331:1335	arg1	healing					1337:1343	The in vivo wound healing	1319:1343	The in vivo wound healing of rats	1319:1351	The in vivo wound healing of rats showed that this composite hydrogel presented a better healing effect with a wound contraction of 96% at 14 d, less inflammation and higher angiogenesis, than all control groups.
32600722	4	54	theme	NIPAM-co-AA	794:804	arg1	hydrogel					807:814	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel	758:814	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel	758:814	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	3	55	theme	[p	693:694	arg1	NGs					709:711	N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs	645:711	N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs	645:711	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	55	theme	[p	693:694	arg1	~40 °C					720:725	LCST2 ~40 °C	714:725	LCST2 ~40 °C	714:725	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	0	56	with	dressing	65:72	arg1	delivery					88:95	stepwise delivery	79:95	stepwise delivery of drug and growth factor for wound repair	79:138	An alginate/poly(N-isopropylacrylamide)-based composite hydrogel dressing with stepwise delivery of drug and growth factor for wound repair.
32600722	3	57	theme	calcium	490:496	arg1	ion					498:500	calcium ion	490:500	calcium ion	490:500	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	6	58	from	angiogenesis	1493:1504	arg1	d					1461:1461	14 d	1458:1461	14 d	1458:1461	The in vivo wound healing of rats showed that this composite hydrogel presented a better healing effect with a wound contraction of 96% at 14 d, less inflammation and higher angiogenesis, than all control groups.
32600722	4	59	theme	SA/bFGF	774:780	arg1	hydrogel					807:814	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel	758:814	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel	758:814	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	5	60	theme	tissue	1295:1300	arg1	formation					1302:1310	new tissue formation stage	1291:1316	new tissue formation stage	1291:1316	The in vitro thermosensitive cargo delivery of this hydrogel showed that 92% of DS was sustainably delivered at 37 °C within the early three days mimicking the inflammation stage, while 80% of bFGF was controlled released at 25 °C within the later eight days mimicking new tissue formation stage.
32600722	2	61	theme	sodium	312:317	arg1	delivery					289:296	stepwise delivery	280:296	stepwise delivery of diclofenac sodium (DS) and basic fibroblast growth factor (bFGF)	280:364	In this study, we developed a composite hydrogel dressing with stepwise delivery of diclofenac sodium (DS) and basic fibroblast growth factor (bFGF) in the inflammation stage and new tissue formation stage respectively for wound repair.
32600722	6	62	theme	healing	1408:1414	arg1	effect					1416:1421	a better healing effect	1399:1421	a better healing effect	1399:1421	The in vivo wound healing of rats showed that this composite hydrogel presented a better healing effect with a wound contraction of 96% at 14 d, less inflammation and higher angiogenesis, than all control groups.
32600722	1	63	theme	essential	184:192	arg1	role					194:197	an essential role	181:197	an essential role	181:197	Anti-inflammation and angiogenesis play an essential role in wound healing.
32600722	4	64	theme	pNIPAM/DS	782:790	arg1	hydrogel					807:814	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel	758:814	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel	758:814	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	4	65	theme	storage	838:844	arg1	modulus					846:852	a desirable storage modulus	826:852	a desirable storage modulus of ~4500 Pa	826:864	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	3	66	theme	NIPAM-co-AA	696:706	arg1	NGs					709:711	N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs	645:711	N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs	645:711	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	66	theme	NIPAM-co-AA	696:706	arg1	~40 °C					720:725	LCST2 ~40 °C	714:725	LCST2 ~40 °C	714:725	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	67	theme	bFGF-loaded	553:563	arg1	p					643:643	DS-loaded p	633:643	DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C]	633:726	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	67	theme	bFGF-loaded	553:563	arg1	phase					751:755	the dispersed phase	737:755	the dispersed phase	737:755	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	67	theme	bFGF-loaded	553:563	arg1	nanogels					593:600	thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels	537:600	thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C)	537:627	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	68	theme	LCST2	714:718	arg1	NGs					709:711	N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs	645:711	N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs	645:711	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	68	theme	LCST2	714:718	arg1	~40 °C					720:725	LCST2 ~40 °C	714:725	LCST2 ~40 °C	714:725	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	2	69	theme	composite	247:255	arg1	dressing					266:273	a composite hydrogel dressing	245:273	a composite hydrogel dressing with stepwise delivery of diclofenac sodium (DS) and basic fibroblast growth factor (bFGF)	245:364	In this study, we developed a composite hydrogel dressing with stepwise delivery of diclofenac sodium (DS) and basic fibroblast growth factor (bFGF) in the inflammation stage and new tissue formation stage respectively for wound repair.
32600722	6	70	theme	%	1453:1453	arg1	inflammation					1469:1480	less inflammation	1464:1480	less inflammation	1464:1480	The in vivo wound healing of rats showed that this composite hydrogel presented a better healing effect with a wound contraction of 96% at 14 d, less inflammation and higher angiogenesis, than all control groups.
32600722	6	70	theme	%	1453:1453	arg1	angiogenesis					1493:1504	higher angiogenesis	1486:1504	higher angiogenesis	1486:1504	The in vivo wound healing of rats showed that this composite hydrogel presented a better healing effect with a wound contraction of 96% at 14 d, less inflammation and higher angiogenesis, than all control groups.
32600722	6	70	theme	%	1453:1453	arg1	contraction					1436:1446	a wound contraction	1428:1446	a wound contraction of 96% at 14 d	1428:1461	The in vivo wound healing of rats showed that this composite hydrogel presented a better healing effect with a wound contraction of 96% at 14 d, less inflammation and higher angiogenesis, than all control groups.
32600722	6	71	from	d	1461:1461	arg1	inflammation					1469:1480	less inflammation	1464:1480	less inflammation	1464:1480	The in vivo wound healing of rats showed that this composite hydrogel presented a better healing effect with a wound contraction of 96% at 14 d, less inflammation and higher angiogenesis, than all control groups.
32600722	6	71	from	d	1461:1461	arg1	angiogenesis					1493:1504	higher angiogenesis	1486:1504	higher angiogenesis	1486:1504	The in vivo wound healing of rats showed that this composite hydrogel presented a better healing effect with a wound contraction of 96% at 14 d, less inflammation and higher angiogenesis, than all control groups.
32600722	6	71	from	d	1461:1461	arg1	contraction					1436:1446	a wound contraction	1428:1446	a wound contraction of 96% at 14 d	1428:1461	The in vivo wound healing of rats showed that this composite hydrogel presented a better healing effect with a wound contraction of 96% at 14 d, less inflammation and higher angiogenesis, than all control groups.
32600722	6	71	from	d	1461:1461	arg1	%					1453:1453	96%	1451:1453	96% at 14 d	1451:1461	The in vivo wound healing of rats showed that this composite hydrogel presented a better healing effect with a wound contraction of 96% at 14 d, less inflammation and higher angiogenesis, than all control groups.
32600722	7	72	theme	composite	1589:1597	arg1	hydrogel					1599:1606	SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel	1556:1606	SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel	1556:1606	These findings indicate SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel is a potential dressing for wound repair.
32600722	7	72	theme	composite	1589:1597	arg1	dressing					1623:1630	a potential dressing	1611:1630	a potential dressing for wound repair	1611:1647	These findings indicate SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel is a potential dressing for wound repair.
32600722	2	73	with	dressing	266:273	arg1	delivery					289:296	stepwise delivery	280:296	stepwise delivery of diclofenac sodium (DS) and basic fibroblast growth factor (bFGF)	280:364	In this study, we developed a composite hydrogel dressing with stepwise delivery of diclofenac sodium (DS) and basic fibroblast growth factor (bFGF) in the inflammation stage and new tissue formation stage respectively for wound repair.
32600722	6	74	theme	control	1516:1522	arg1	groups					1524:1529	all control groups	1512:1529	all control groups	1512:1529	The in vivo wound healing of rats showed that this composite hydrogel presented a better healing effect with a wound contraction of 96% at 14 d, less inflammation and higher angiogenesis, than all control groups.
32600722	3	75	theme	dispersed	741:749	arg1	p					643:643	DS-loaded p	633:643	DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C]	633:726	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	75	theme	dispersed	741:749	arg1	phase					751:755	the dispersed phase	737:755	the dispersed phase	737:755	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	75	theme	dispersed	741:749	arg1	nanogels					593:600	thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels	537:600	thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C)	537:627	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	6	76	from	contraction	1436:1446	arg1	d					1461:1461	14 d	1458:1461	14 d	1458:1461	The in vivo wound healing of rats showed that this composite hydrogel presented a better healing effect with a wound contraction of 96% at 14 d, less inflammation and higher angiogenesis, than all control groups.
32600722	7	77	theme	NIPAM-co-AA	1576:1586	arg1	hydrogel					1599:1606	SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel	1556:1606	SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel	1556:1606	These findings indicate SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel is a potential dressing for wound repair.
32600722	7	77	theme	NIPAM-co-AA	1576:1586	arg1	dressing					1623:1630	a potential dressing	1611:1630	a potential dressing for wound repair	1611:1647	These findings indicate SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel is a potential dressing for wound repair.
32600722	4	78	theme	equilibrium	880:890	arg1	ratio					901:905	a high water equilibrium swelling ratio	867:905	a high water equilibrium swelling ratio of ~90	867:912	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	5	79	theme	early	1151:1155	arg1	days					1163:1166	the early three days	1147:1166	the early three days mimicking the inflammation stage	1147:1199	The in vitro thermosensitive cargo delivery of this hydrogel showed that 92% of DS was sustainably delivered at 37 °C within the early three days mimicking the inflammation stage, while 80% of bFGF was controlled released at 25 °C within the later eight days mimicking new tissue formation stage.
32600722	5	80	theme	thermosensitive	1035:1049	arg1	delivery					1057:1064	The in vitro thermosensitive cargo delivery	1022:1064	The in vitro thermosensitive cargo delivery of this hydrogel	1022:1081	The in vitro thermosensitive cargo delivery of this hydrogel showed that 92% of DS was sustainably delivered at 37 °C within the early three days mimicking the inflammation stage, while 80% of bFGF was controlled released at 25 °C within the later eight days mimicking new tissue formation stage.
32600722	6	81	theme	wound	1430:1434	arg1	contraction					1436:1446	a wound contraction	1428:1446	a wound contraction of 96% at 14 d	1428:1461	The in vivo wound healing of rats showed that this composite hydrogel presented a better healing effect with a wound contraction of 96% at 14 d, less inflammation and higher angiogenesis, than all control groups.
32600722	7	82	theme	p	1574:1574	arg1	hydrogel					1599:1606	SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel	1556:1606	SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel	1556:1606	These findings indicate SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel is a potential dressing for wound repair.
32600722	7	82	theme	p	1574:1574	arg1	dressing					1623:1630	a potential dressing	1611:1630	a potential dressing for wound repair	1611:1647	These findings indicate SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel is a potential dressing for wound repair.
32600722	0	83	theme	drug	100:103	arg1	delivery					88:95	stepwise delivery	79:95	stepwise delivery of drug and growth factor for wound repair	79:138	An alginate/poly(N-isopropylacrylamide)-based composite hydrogel dressing with stepwise delivery of drug and growth factor for wound repair.
32600722	7	84	theme	pNIPAM/DS	1564:1572	arg1	hydrogel					1599:1606	SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel	1556:1606	SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel	1556:1606	These findings indicate SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel is a potential dressing for wound repair.
32600722	7	84	theme	pNIPAM/DS	1564:1572	arg1	dressing					1623:1630	a potential dressing	1611:1630	a potential dressing for wound repair	1611:1647	These findings indicate SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel is a potential dressing for wound repair.
32600722	5	85	theme	bFGF	1215:1218	arg1	%					1210:1210	80%	1208:1210	80% of bFGF	1208:1218	The in vitro thermosensitive cargo delivery of this hydrogel showed that 92% of DS was sustainably delivered at 37 °C within the early three days mimicking the inflammation stage, while 80% of bFGF was controlled released at 25 °C within the later eight days mimicking new tissue formation stage.
32600722	5	85	theme	bFGF	1215:1218	arg1	bFGF					1215:1218	bFGF	1215:1218	bFGF	1215:1218	The in vitro thermosensitive cargo delivery of this hydrogel showed that 92% of DS was sustainably delivered at 37 °C within the early three days mimicking the inflammation stage, while 80% of bFGF was controlled released at 25 °C within the later eight days mimicking new tissue formation stage.
32600722	0	86	theme	growth	109:114	arg1	factor					116:121	growth factor	109:121	growth factor	109:121	An alginate/poly(N-isopropylacrylamide)-based composite hydrogel dressing with stepwise delivery of drug and growth factor for wound repair.
32600722	6	87	theme	higher	1486:1491	arg1	angiogenesis					1493:1504	higher angiogenesis	1486:1504	higher angiogenesis	1486:1504	The in vivo wound healing of rats showed that this composite hydrogel presented a better healing effect with a wound contraction of 96% at 14 d, less inflammation and higher angiogenesis, than all control groups.
32600722	7	88	theme	SA/bFGF	1556:1562	arg1	hydrogel					1599:1606	SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel	1556:1606	SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel	1556:1606	These findings indicate SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel is a potential dressing for wound repair.
32600722	7	88	theme	SA/bFGF	1556:1562	arg1	dressing					1623:1630	a potential dressing	1611:1630	a potential dressing for wound repair	1611:1647	These findings indicate SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel is a potential dressing for wound repair.
32600722	4	89	theme	high	869:872	arg1	ratio					901:905	a high water equilibrium swelling ratio	867:905	a high water equilibrium swelling ratio of ~90	867:912	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	5	90	theme	in	1026:1027	arg1	delivery					1057:1064	The in vitro thermosensitive cargo delivery	1022:1064	The in vitro thermosensitive cargo delivery of this hydrogel	1022:1081	The in vitro thermosensitive cargo delivery of this hydrogel showed that 92% of DS was sustainably delivered at 37 °C within the early three days mimicking the inflammation stage, while 80% of bFGF was controlled released at 25 °C within the later eight days mimicking new tissue formation stage.
32600722	4	91	theme	water	930:934	arg1	rate					955:958	an appropriate water vapor transmission rate	915:958	an appropriate water vapor transmission rate of ~2300 g/m2/day	915:976	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	3	92	dep	p	643:643	arg1	NGs					709:711	N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs	645:711	N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs	645:711	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	92	dep	p	643:643	arg1	~40 °C					720:725	LCST2 ~40 °C	714:725	LCST2 ~40 °C	714:725	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	2	93	theme	new	396:398	arg1	formation					407:415	new tissue formation	396:415	new tissue formation	396:415	In this study, we developed a composite hydrogel dressing with stepwise delivery of diclofenac sodium (DS) and basic fibroblast growth factor (bFGF) in the inflammation stage and new tissue formation stage respectively for wound repair.
32600722	0	94	theme	-based	39:44	arg1	dressing					65:72	An alginate/poly(N-isopropylacrylamide)-based composite hydrogel dressing	0:72	An alginate/poly(N-isopropylacrylamide)-based composite hydrogel dressing with stepwise delivery of drug and growth factor for wound repair.	0:139	An alginate/poly(N-isopropylacrylamide)-based composite hydrogel dressing with stepwise delivery of drug and growth factor for wound repair.
32600722	4	95	theme	~90	910:912	arg1	modulus					846:852	a desirable storage modulus	826:852	a desirable storage modulus of ~4500 Pa	826:864	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	4	95	theme	~90	910:912	arg1	ratio					901:905	a high water equilibrium swelling ratio	867:905	a high water equilibrium swelling ratio of ~90	867:912	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	4	95	theme	~90	910:912	arg1	nontoxicity					983:993	nontoxicity	983:993	nontoxicity to human skin fibroblasts	983:1019	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	4	95	theme	~90	910:912	arg1	rate					955:958	an appropriate water vapor transmission rate	915:958	an appropriate water vapor transmission rate of ~2300 g/m2/day	915:976	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	5	96	theme	later	1264:1268	arg1	days					1276:1279	the later eight days	1260:1279	the later eight days mimicking new tissue formation stage	1260:1316	The in vitro thermosensitive cargo delivery of this hydrogel showed that 92% of DS was sustainably delivered at 37 °C within the early three days mimicking the inflammation stage, while 80% of bFGF was controlled released at 25 °C within the later eight days mimicking new tissue formation stage.
32600722	0	97	theme	hydrogel	56:63	arg1	dressing					65:72	An alginate/poly(N-isopropylacrylamide)-based composite hydrogel dressing	0:72	An alginate/poly(N-isopropylacrylamide)-based composite hydrogel dressing with stepwise delivery of drug and growth factor for wound repair.	0:139	An alginate/poly(N-isopropylacrylamide)-based composite hydrogel dressing with stepwise delivery of drug and growth factor for wound repair.
32600722	2	98	dep	stage	386:390	arg1	stage					417:421	stage	417:421	stage	417:421	In this study, we developed a composite hydrogel dressing with stepwise delivery of diclofenac sodium (DS) and basic fibroblast growth factor (bFGF) in the inflammation stage and new tissue formation stage respectively for wound repair.
32600722	2	98	dep	stage	386:390	arg1	the					369:371	the	369:371	the	369:371	In this study, we developed a composite hydrogel dressing with stepwise delivery of diclofenac sodium (DS) and basic fibroblast growth factor (bFGF) in the inflammation stage and new tissue formation stage respectively for wound repair.
32600722	6	99	theme	composite	1370:1378	arg1	hydrogel					1380:1387	this composite hydrogel	1365:1387	this composite hydrogel	1365:1387	The in vivo wound healing of rats showed that this composite hydrogel presented a better healing effect with a wound contraction of 96% at 14 d, less inflammation and higher angiogenesis, than all control groups.
32600722	3	100	theme	LCST1	615:619	arg1	NGs					610:612	pNIPAM NGs	603:612	pNIPAM NGs	603:612	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	100	theme	LCST1	615:619	arg1	~33 °C					621:626	LCST1 ~33 °C	615:626	LCST1 ~33 °C	615:626	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	101	theme	Sodium	454:459	arg1	alginate					461:468	Sodium alginate	454:468	Sodium alginate (SA) crosslinked by calcium ion	454:500	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	101	theme	Sodium	454:459	arg1	phase					526:530	the continuous phase	511:530	the continuous phase	511:530	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	3	101	theme	Sodium	454:459	arg1	SA					471:472	SA	471:472	SA	471:472	Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase.
32600722	6	102	from	inflammation	1469:1480	arg1	d					1461:1461	14 d	1458:1461	14 d	1458:1461	The in vivo wound healing of rats showed that this composite hydrogel presented a better healing effect with a wound contraction of 96% at 14 d, less inflammation and higher angiogenesis, than all control groups.
32600722	4	103	theme	human	998:1002	arg1	fibroblasts					1009:1019	human skin fibroblasts	998:1019	human skin fibroblasts	998:1019	The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts.
32600722	2	104	theme	growth	345:350	arg1	bFGF					360:363	bFGF	360:363	bFGF	360:363	In this study, we developed a composite hydrogel dressing with stepwise delivery of diclofenac sodium (DS) and basic fibroblast growth factor (bFGF) in the inflammation stage and new tissue formation stage respectively for wound repair.
32600722	2	104	theme	growth	345:350	arg1	factor					352:357	basic fibroblast growth factor	328:357	basic fibroblast growth factor (bFGF)	328:364	In this study, we developed a composite hydrogel dressing with stepwise delivery of diclofenac sodium (DS) and basic fibroblast growth factor (bFGF) in the inflammation stage and new tissue formation stage respectively for wound repair.
32600722	6	105	theme	rats	1348:1351	arg1	healing					1337:1343	The in vivo wound healing	1319:1343	The in vivo wound healing of rats	1319:1351	The in vivo wound healing of rats showed that this composite hydrogel presented a better healing effect with a wound contraction of 96% at 14 d, less inflammation and higher angiogenesis, than all control groups.
33410539	2	0	theme	biallelic	409:417	arg1	variants					419:426	biallelic variants	409:426	biallelic variants in the PIGS gene, of whom two presented with fetal akinesia	409:486	Previous studies described seven patients with biallelic variants in the PIGS gene, of whom two presented with fetal akinesia and five with global developmental delay and epileptic developmental encephalopathy.
33410539	1	1	theme	recessive	275:283	arg1	deficiency					350:359	autosomal recessive inherited glycosylphosphatidylinositol-anchored protein (GPI-AP) deficiency	265:359	autosomal recessive inherited glycosylphosphatidylinositol-anchored protein (GPI-AP) deficiency	265:359	The phosphatidylinositol glycan anchor biosynthesis class S protein (PIGS) gene has recently been implicated in a novel congenital disorder of glycosylation resulting in autosomal recessive inherited glycosylphosphatidylinositol-anchored protein (GPI-AP) deficiency.
33410539	5	2	theme	facial	1046:1051	arg1	gestalt					1053:1059	a distinctive facial gestalt	1032:1059	a distinctive facial gestalt	1032:1059	Additional findings include vision impairment, hearing loss, renal malformation, and hypotonic facial appearances with minor dysmorphic features but without a distinctive facial gestalt.
33410539	1	3	theme	glycosylphosphatidylinositol-anchored	295:331	arg1	GPI-AP					342:347	GPI-AP	342:347	GPI-AP	342:347	The phosphatidylinositol glycan anchor biosynthesis class S protein (PIGS) gene has recently been implicated in a novel congenital disorder of glycosylation resulting in autosomal recessive inherited glycosylphosphatidylinositol-anchored protein (GPI-AP) deficiency.
33410539	1	3	theme	glycosylphosphatidylinositol-anchored	295:331	arg1	protein					333:339	glycosylphosphatidylinositol-anchored protein	295:339	autosomal recessive inherited glycosylphosphatidylinositol-anchored protein (GPI-AP) deficiency	265:359	The phosphatidylinositol glycan anchor biosynthesis class S protein (PIGS) gene has recently been implicated in a novel congenital disorder of glycosylation resulting in autosomal recessive inherited glycosylphosphatidylinositol-anchored protein (GPI-AP) deficiency.
33410539	8	4	theme	GPI	1316:1318	arg1	deficiency					1320:1329	inherited GPI deficiency	1306:1329	inherited GPI deficiency	1306:1329	We confirm that biallelic variants in PIGS cause vitamin pyridoxine-responsive epilepsy due to inherited GPI deficiency and expand the genotype and phenotype of PIGS-related disorder.
33410539	1	5	theme	class	147:151	arg1	gene					170:173	biosynthesis class S protein (PIGS) gene	134:173	The phosphatidylinositol glycan anchor biosynthesis class S protein (PIGS) gene	95:173	The phosphatidylinositol glycan anchor biosynthesis class S protein (PIGS) gene has recently been implicated in a novel congenital disorder of glycosylation resulting in autosomal recessive inherited glycosylphosphatidylinositol-anchored protein (GPI-AP) deficiency.
33410539	8	6	theme	disorder	1385:1392	arg1	phenotype					1359:1367	phenotype	1359:1367	phenotype	1359:1367	We confirm that biallelic variants in PIGS cause vitamin pyridoxine-responsive epilepsy due to inherited GPI deficiency and expand the genotype and phenotype of PIGS-related disorder.
33410539	8	6	theme	disorder	1385:1392	arg1	genotype					1346:1353	genotype	1346:1353	genotype	1346:1353	We confirm that biallelic variants in PIGS cause vitamin pyridoxine-responsive epilepsy due to inherited GPI deficiency and expand the genotype and phenotype of PIGS-related disorder.
33410539	5	7	theme	hypotonic	960:968	arg1	appearances					977:987	hypotonic facial appearances	960:987	hypotonic facial appearances with minor dysmorphic features but without a distinctive facial gestalt	960:1059	Additional findings include vision impairment, hearing loss, renal malformation, and hypotonic facial appearances with minor dysmorphic features but without a distinctive facial gestalt.
33410539	4	8	theme	severe	752:757	arg1	delay					780:784	severe global developmental delay	752:784	severe global developmental delay	752:784	All individuals presented with hypotonia, severe global developmental delay, microcephaly, intractable early infantile epilepsy, and structural brain abnormalities.
33410539	2	9	theme	Previous	362:369	arg1	studies					371:377	Previous studies	362:377	Previous studies	362:377	Previous studies described seven patients with biallelic variants in the PIGS gene, of whom two presented with fetal akinesia and five with global developmental delay and epileptic developmental encephalopathy.
33410539	5	10	with	impairment	910:919	arg1	features					1011:1018	minor dysmorphic features	994:1018	minor dysmorphic features	994:1018	Additional findings include vision impairment, hearing loss, renal malformation, and hypotonic facial appearances with minor dysmorphic features but without a distinctive facial gestalt.
33410539	5	11	theme	renal	936:940	arg1	malformation					942:953	renal malformation	936:953	renal malformation	936:953	Additional findings include vision impairment, hearing loss, renal malformation, and hypotonic facial appearances with minor dysmorphic features but without a distinctive facial gestalt.
33410539	7	12	theme	anchoring	1121:1129	arg1	studies					1131:1137	GPI anchoring studies	1117:1137	GPI anchoring studies performed on one individual	1117:1165	GPI anchoring studies performed on one individual revealed a significant decrease in GPI-APs.
33410539	1	13	theme	S	153:153	arg1	gene					170:173	biosynthesis class S protein (PIGS) gene	134:173	The phosphatidylinositol glycan anchor biosynthesis class S protein (PIGS) gene	95:173	The phosphatidylinositol glycan anchor biosynthesis class S protein (PIGS) gene has recently been implicated in a novel congenital disorder of glycosylation resulting in autosomal recessive inherited glycosylphosphatidylinositol-anchored protein (GPI-AP) deficiency.
33410539	4	14	theme	early	813:817	arg1	epilepsy					829:836	intractable early infantile epilepsy	801:836	intractable early infantile epilepsy	801:836	All individuals presented with hypotonia, severe global developmental delay, microcephaly, intractable early infantile epilepsy, and structural brain abnormalities.
33410539	5	15	theme	distinctive	1034:1044	arg1	gestalt					1053:1059	a distinctive facial gestalt	1032:1059	a distinctive facial gestalt	1032:1059	Additional findings include vision impairment, hearing loss, renal malformation, and hypotonic facial appearances with minor dysmorphic features but without a distinctive facial gestalt.
33410539	4	16	theme	developmental	766:778	arg1	delay					780:784	severe global developmental delay	752:784	severe global developmental delay	752:784	All individuals presented with hypotonia, severe global developmental delay, microcephaly, intractable early infantile epilepsy, and structural brain abnormalities.
33410539	0	17	theme	developmental	65:77	arg1	encephalopathy					79:92	PIGS-associated early onset epileptic developmental encephalopathy	27:92	PIGS-associated early onset epileptic developmental encephalopathy	27:92	Expanding the phenotype of PIGS-associated early onset epileptic developmental encephalopathy.
33410539	9	18	theme	spectrum	1432:1439	arg1	delineation					1403:1413	Further delineation	1395:1413	Further delineation of the molecular spectrum of PIGS-related disorders	1395:1465	Further delineation of the molecular spectrum of PIGS-related disorders would improve management, help develop treatments, and encourage the expansion of diagnostic genetic testing to include this gene as a potential cause of neurodevelopmental disorders and epilepsy.
33410539	5	19	theme	minor	994:998	arg1	features					1011:1018	minor dysmorphic features	994:1018	minor dysmorphic features	994:1018	Additional findings include vision impairment, hearing loss, renal malformation, and hypotonic facial appearances with minor dysmorphic features but without a distinctive facial gestalt.
33410539	2	20	theme	epileptic	533:541	arg1	encephalopathy					557:570	epileptic developmental encephalopathy	533:570	epileptic developmental encephalopathy	533:570	Previous studies described seven patients with biallelic variants in the PIGS gene, of whom two presented with fetal akinesia and five with global developmental delay and epileptic developmental encephalopathy.
33410539	9	21	theme	PIGS-related	1444:1455	arg1	disorders					1457:1465	PIGS-related disorders	1444:1465	PIGS-related disorders	1444:1465	Further delineation of the molecular spectrum of PIGS-related disorders would improve management, help develop treatments, and encourage the expansion of diagnostic genetic testing to include this gene as a potential cause of neurodevelopmental disorders and epilepsy.
33410539	4	22	theme	brain	854:858	arg1	abnormalities					860:872	structural brain abnormalities	843:872	structural brain abnormalities	843:872	All individuals presented with hypotonia, severe global developmental delay, microcephaly, intractable early infantile epilepsy, and structural brain abnormalities.
33410539	3	23	from	characteristics	611:625	arg1	families					667:674	five families	662:674	five families	662:674	We present the molecular and clinical characteristics of six additional individuals from five families with unreported variants in PIGS.
33410539	6	24	theme	neurologic	1091:1100	arg1	complications					1102:1114	neurologic complications	1091:1114	neurologic complications	1091:1114	Four individuals died due to neurologic complications.
33410539	3	25	from	variants	692:699	arg1	PIGS					704:707	PIGS	704:707	PIGS	704:707	We present the molecular and clinical characteristics of six additional individuals from five families with unreported variants in PIGS.
33410539	0	26	theme	early	43:47	arg1	encephalopathy					79:92	PIGS-associated early onset epileptic developmental encephalopathy	27:92	PIGS-associated early onset epileptic developmental encephalopathy	27:92	Expanding the phenotype of PIGS-associated early onset epileptic developmental encephalopathy.
33410539	1	27	theme	protein	333:339	arg1	deficiency					350:359	autosomal recessive inherited glycosylphosphatidylinositol-anchored protein (GPI-AP) deficiency	265:359	autosomal recessive inherited glycosylphosphatidylinositol-anchored protein (GPI-AP) deficiency	265:359	The phosphatidylinositol glycan anchor biosynthesis class S protein (PIGS) gene has recently been implicated in a novel congenital disorder of glycosylation resulting in autosomal recessive inherited glycosylphosphatidylinositol-anchored protein (GPI-AP) deficiency.
33410539	1	28	theme	glycan	120:125	arg1	anchor					127:132	The phosphatidylinositol glycan anchor biosynthesis class S protein (PIGS) gene	95:173	The phosphatidylinositol glycan anchor biosynthesis class S protein (PIGS) gene	95:173	The phosphatidylinositol glycan anchor biosynthesis class S protein (PIGS) gene has recently been implicated in a novel congenital disorder of glycosylation resulting in autosomal recessive inherited glycosylphosphatidylinositol-anchored protein (GPI-AP) deficiency.
33410539	5	29	with	malformation	942:953	arg1	features					1011:1018	minor dysmorphic features	994:1018	minor dysmorphic features	994:1018	Additional findings include vision impairment, hearing loss, renal malformation, and hypotonic facial appearances with minor dysmorphic features but without a distinctive facial gestalt.
33410539	3	30	with	individuals	645:655	arg1	variants					692:699	unreported variants	681:699	unreported variants in PIGS	681:707	We present the molecular and clinical characteristics of six additional individuals from five families with unreported variants in PIGS.
33410539	1	31	theme	biosynthesis	134:145	arg1	gene					170:173	biosynthesis class S protein (PIGS) gene	134:173	The phosphatidylinositol glycan anchor biosynthesis class S protein (PIGS) gene	95:173	The phosphatidylinositol glycan anchor biosynthesis class S protein (PIGS) gene has recently been implicated in a novel congenital disorder of glycosylation resulting in autosomal recessive inherited glycosylphosphatidylinositol-anchored protein (GPI-AP) deficiency.
33410539	1	32	dep	anchor	127:132	arg1	gene					170:173	biosynthesis class S protein (PIGS) gene	134:173	The phosphatidylinositol glycan anchor biosynthesis class S protein (PIGS) gene	95:173	The phosphatidylinositol glycan anchor biosynthesis class S protein (PIGS) gene has recently been implicated in a novel congenital disorder of glycosylation resulting in autosomal recessive inherited glycosylphosphatidylinositol-anchored protein (GPI-AP) deficiency.
33410539	0	33	theme	PIGS-associated	27:41	arg1	encephalopathy					79:92	PIGS-associated early onset epileptic developmental encephalopathy	27:92	PIGS-associated early onset epileptic developmental encephalopathy	27:92	Expanding the phenotype of PIGS-associated early onset epileptic developmental encephalopathy.
33410539	2	34	theme	global	502:507	arg1	delay					523:527	global developmental delay	502:527	global developmental delay	502:527	Previous studies described seven patients with biallelic variants in the PIGS gene, of whom two presented with fetal akinesia and five with global developmental delay and epileptic developmental encephalopathy.
33410539	5	35	theme	vision	903:908	arg1	impairment					910:919	vision impairment	903:919	vision impairment	903:919	Additional findings include vision impairment, hearing loss, renal malformation, and hypotonic facial appearances with minor dysmorphic features but without a distinctive facial gestalt.
33410539	8	36	theme	biallelic	1227:1235	arg1	variants					1237:1244	biallelic variants	1227:1244	biallelic variants in PIGS	1227:1252	We confirm that biallelic variants in PIGS cause vitamin pyridoxine-responsive epilepsy due to inherited GPI deficiency and expand the genotype and phenotype of PIGS-related disorder.
33410539	9	37	theme	genetic	1560:1566	arg1	testing					1568:1574	diagnostic genetic testing	1549:1574	diagnostic genetic testing	1549:1574	Further delineation of the molecular spectrum of PIGS-related disorders would improve management, help develop treatments, and encourage the expansion of diagnostic genetic testing to include this gene as a potential cause of neurodevelopmental disorders and epilepsy.
33410539	8	38	theme	pyridoxine-responsive	1268:1288	arg1	epilepsy					1290:1297	vitamin pyridoxine-responsive epilepsy	1260:1297	vitamin pyridoxine-responsive epilepsy due to inherited GPI deficiency	1260:1329	We confirm that biallelic variants in PIGS cause vitamin pyridoxine-responsive epilepsy due to inherited GPI deficiency and expand the genotype and phenotype of PIGS-related disorder.
33410539	7	39	from	decrease	1190:1197	arg1	GPI-APs					1202:1208	GPI-APs	1202:1208	GPI-APs	1202:1208	GPI anchoring studies performed on one individual revealed a significant decrease in GPI-APs.
33410539	1	40	theme	novel	209:213	arg1	disorder					226:233	a novel congenital disorder	207:233	a novel congenital disorder of glycosylation resulting in autosomal recessive inherited glycosylphosphatidylinositol-anchored protein (GPI-AP) deficiency	207:359	The phosphatidylinositol glycan anchor biosynthesis class S protein (PIGS) gene has recently been implicated in a novel congenital disorder of glycosylation resulting in autosomal recessive inherited glycosylphosphatidylinositol-anchored protein (GPI-AP) deficiency.
33410539	3	41	theme	clinical	602:609	arg1	characteristics					611:625	the molecular and clinical characteristics	584:625	the molecular and clinical characteristics of six additional individuals from five families with unreported variants in PIGS	584:707	We present the molecular and clinical characteristics of six additional individuals from five families with unreported variants in PIGS.
33410539	1	42	theme	inherited	285:293	arg1	deficiency					350:359	autosomal recessive inherited glycosylphosphatidylinositol-anchored protein (GPI-AP) deficiency	265:359	autosomal recessive inherited glycosylphosphatidylinositol-anchored protein (GPI-AP) deficiency	265:359	The phosphatidylinositol glycan anchor biosynthesis class S protein (PIGS) gene has recently been implicated in a novel congenital disorder of glycosylation resulting in autosomal recessive inherited glycosylphosphatidylinositol-anchored protein (GPI-AP) deficiency.
33410539	8	43	theme	due	1299:1301	arg1	epilepsy					1290:1297	vitamin pyridoxine-responsive epilepsy	1260:1297	vitamin pyridoxine-responsive epilepsy due to inherited GPI deficiency	1260:1329	We confirm that biallelic variants in PIGS cause vitamin pyridoxine-responsive epilepsy due to inherited GPI deficiency and expand the genotype and phenotype of PIGS-related disorder.
33410539	3	44	theme	molecular	588:596	arg1	characteristics					611:625	the molecular and clinical characteristics	584:625	the molecular and clinical characteristics of six additional individuals from five families with unreported variants in PIGS	584:707	We present the molecular and clinical characteristics of six additional individuals from five families with unreported variants in PIGS.
33410539	5	45	theme	facial	970:975	arg1	appearances					977:987	hypotonic facial appearances	960:987	hypotonic facial appearances with minor dysmorphic features but without a distinctive facial gestalt	960:1059	Additional findings include vision impairment, hearing loss, renal malformation, and hypotonic facial appearances with minor dysmorphic features but without a distinctive facial gestalt.
33410539	8	46	theme	inherited	1306:1314	arg1	deficiency					1320:1329	inherited GPI deficiency	1306:1329	inherited GPI deficiency	1306:1329	We confirm that biallelic variants in PIGS cause vitamin pyridoxine-responsive epilepsy due to inherited GPI deficiency and expand the genotype and phenotype of PIGS-related disorder.
33410539	2	47	from	variants	419:426	arg1	gene					440:443	the PIGS gene	431:443	the PIGS gene	431:443	Previous studies described seven patients with biallelic variants in the PIGS gene, of whom two presented with fetal akinesia and five with global developmental delay and epileptic developmental encephalopathy.
33410539	8	48	theme	PIGS-related	1372:1383	arg1	disorder					1385:1392	PIGS-related disorder	1372:1392	PIGS-related disorder	1372:1392	We confirm that biallelic variants in PIGS cause vitamin pyridoxine-responsive epilepsy due to inherited GPI deficiency and expand the genotype and phenotype of PIGS-related disorder.
33410539	3	49	theme	additional	634:643	arg1	individuals					645:655	six additional individuals	630:655	six additional individuals from five families with unreported variants in PIGS	630:707	We present the molecular and clinical characteristics of six additional individuals from five families with unreported variants in PIGS.
33410539	9	50	theme	potential	1602:1610	arg1	cause					1612:1616	a potential cause	1600:1616	a potential cause of neurodevelopmental disorders and epilepsy	1600:1661	Further delineation of the molecular spectrum of PIGS-related disorders would improve management, help develop treatments, and encourage the expansion of diagnostic genetic testing to include this gene as a potential cause of neurodevelopmental disorders and epilepsy.
33410539	9	50	theme	potential	1602:1610	arg1	gene					1592:1595	this gene	1587:1595	this gene	1587:1595	Further delineation of the molecular spectrum of PIGS-related disorders would improve management, help develop treatments, and encourage the expansion of diagnostic genetic testing to include this gene as a potential cause of neurodevelopmental disorders and epilepsy.
33410539	5	51	with	loss	930:933	arg1	features					1011:1018	minor dysmorphic features	994:1018	minor dysmorphic features	994:1018	Additional findings include vision impairment, hearing loss, renal malformation, and hypotonic facial appearances with minor dysmorphic features but without a distinctive facial gestalt.
33410539	0	52	theme	onset	49:53	arg1	encephalopathy					79:92	PIGS-associated early onset epileptic developmental encephalopathy	27:92	PIGS-associated early onset epileptic developmental encephalopathy	27:92	Expanding the phenotype of PIGS-associated early onset epileptic developmental encephalopathy.
33410539	3	53	theme	individuals	645:655	arg1	characteristics					611:625	the molecular and clinical characteristics	584:625	the molecular and clinical characteristics of six additional individuals from five families with unreported variants in PIGS	584:707	We present the molecular and clinical characteristics of six additional individuals from five families with unreported variants in PIGS.
33410539	7	54	theme	significant	1178:1188	arg1	decrease					1190:1197	a significant decrease	1176:1197	a significant decrease in GPI-APs	1176:1208	GPI anchoring studies performed on one individual revealed a significant decrease in GPI-APs.
33410539	9	55	theme	disorders	1640:1648	arg1	cause					1612:1616	a potential cause	1600:1616	a potential cause of neurodevelopmental disorders and epilepsy	1600:1661	Further delineation of the molecular spectrum of PIGS-related disorders would improve management, help develop treatments, and encourage the expansion of diagnostic genetic testing to include this gene as a potential cause of neurodevelopmental disorders and epilepsy.
33410539	9	55	theme	disorders	1640:1648	arg1	gene					1592:1595	this gene	1587:1595	this gene	1587:1595	Further delineation of the molecular spectrum of PIGS-related disorders would improve management, help develop treatments, and encourage the expansion of diagnostic genetic testing to include this gene as a potential cause of neurodevelopmental disorders and epilepsy.
33410539	9	56	theme	Further	1395:1401	arg1	delineation					1403:1413	Further delineation	1395:1413	Further delineation of the molecular spectrum of PIGS-related disorders	1395:1465	Further delineation of the molecular spectrum of PIGS-related disorders would improve management, help develop treatments, and encourage the expansion of diagnostic genetic testing to include this gene as a potential cause of neurodevelopmental disorders and epilepsy.
33410539	9	57	theme	epilepsy	1654:1661	arg1	cause					1612:1616	a potential cause	1600:1616	a potential cause of neurodevelopmental disorders and epilepsy	1600:1661	Further delineation of the molecular spectrum of PIGS-related disorders would improve management, help develop treatments, and encourage the expansion of diagnostic genetic testing to include this gene as a potential cause of neurodevelopmental disorders and epilepsy.
33410539	9	57	theme	epilepsy	1654:1661	arg1	gene					1592:1595	this gene	1587:1595	this gene	1587:1595	Further delineation of the molecular spectrum of PIGS-related disorders would improve management, help develop treatments, and encourage the expansion of diagnostic genetic testing to include this gene as a potential cause of neurodevelopmental disorders and epilepsy.
33410539	1	58	theme	protein	155:161	arg1	gene					170:173	biosynthesis class S protein (PIGS) gene	134:173	The phosphatidylinositol glycan anchor biosynthesis class S protein (PIGS) gene	95:173	The phosphatidylinositol glycan anchor biosynthesis class S protein (PIGS) gene has recently been implicated in a novel congenital disorder of glycosylation resulting in autosomal recessive inherited glycosylphosphatidylinositol-anchored protein (GPI-AP) deficiency.
33410539	4	59	theme	intractable	801:811	arg1	epilepsy					829:836	intractable early infantile epilepsy	801:836	intractable early infantile epilepsy	801:836	All individuals presented with hypotonia, severe global developmental delay, microcephaly, intractable early infantile epilepsy, and structural brain abnormalities.
33410539	0	60	theme	encephalopathy	79:92	arg1	phenotype					14:22	the phenotype	10:22	the phenotype of PIGS-associated early onset epileptic developmental encephalopathy	10:92	Expanding the phenotype of PIGS-associated early onset epileptic developmental encephalopathy.
33410539	9	61	theme	molecular	1422:1430	arg1	spectrum					1432:1439	the molecular spectrum	1418:1439	the molecular spectrum of PIGS-related disorders	1418:1465	Further delineation of the molecular spectrum of PIGS-related disorders would improve management, help develop treatments, and encourage the expansion of diagnostic genetic testing to include this gene as a potential cause of neurodevelopmental disorders and epilepsy.
33410539	3	62	theme	unreported	681:690	arg1	variants					692:699	unreported variants	681:699	unreported variants in PIGS	681:707	We present the molecular and clinical characteristics of six additional individuals from five families with unreported variants in PIGS.
33410539	7	63	theme	GPI	1117:1119	arg1	studies					1131:1137	GPI anchoring studies	1117:1137	GPI anchoring studies performed on one individual	1117:1165	GPI anchoring studies performed on one individual revealed a significant decrease in GPI-APs.
33410539	1	64	theme	PIGS	164:167	arg1	gene					170:173	biosynthesis class S protein (PIGS) gene	134:173	The phosphatidylinositol glycan anchor biosynthesis class S protein (PIGS) gene	95:173	The phosphatidylinositol glycan anchor biosynthesis class S protein (PIGS) gene has recently been implicated in a novel congenital disorder of glycosylation resulting in autosomal recessive inherited glycosylphosphatidylinositol-anchored protein (GPI-AP) deficiency.
33410539	2	65	theme	developmental	543:555	arg1	encephalopathy					557:570	epileptic developmental encephalopathy	533:570	epileptic developmental encephalopathy	533:570	Previous studies described seven patients with biallelic variants in the PIGS gene, of whom two presented with fetal akinesia and five with global developmental delay and epileptic developmental encephalopathy.
33410539	4	66	theme	global	759:764	arg1	delay					780:784	severe global developmental delay	752:784	severe global developmental delay	752:784	All individuals presented with hypotonia, severe global developmental delay, microcephaly, intractable early infantile epilepsy, and structural brain abnormalities.
33410539	5	67	theme	dysmorphic	1000:1009	arg1	features					1011:1018	minor dysmorphic features	994:1018	minor dysmorphic features	994:1018	Additional findings include vision impairment, hearing loss, renal malformation, and hypotonic facial appearances with minor dysmorphic features but without a distinctive facial gestalt.
33410539	9	68	theme	disorders	1457:1465	arg1	spectrum					1432:1439	the molecular spectrum	1418:1439	the molecular spectrum of PIGS-related disorders	1418:1465	Further delineation of the molecular spectrum of PIGS-related disorders would improve management, help develop treatments, and encourage the expansion of diagnostic genetic testing to include this gene as a potential cause of neurodevelopmental disorders and epilepsy.
33410539	8	69	from	variants	1237:1244	arg1	PIGS					1249:1252	PIGS	1249:1252	PIGS	1249:1252	We confirm that biallelic variants in PIGS cause vitamin pyridoxine-responsive epilepsy due to inherited GPI deficiency and expand the genotype and phenotype of PIGS-related disorder.
33410539	4	70	theme	structural	843:852	arg1	abnormalities					860:872	structural brain abnormalities	843:872	structural brain abnormalities	843:872	All individuals presented with hypotonia, severe global developmental delay, microcephaly, intractable early infantile epilepsy, and structural brain abnormalities.
33410539	9	71	theme	neurodevelopmental	1621:1638	arg1	disorders					1640:1648	neurodevelopmental disorders	1621:1648	neurodevelopmental disorders	1621:1648	Further delineation of the molecular spectrum of PIGS-related disorders would improve management, help develop treatments, and encourage the expansion of diagnostic genetic testing to include this gene as a potential cause of neurodevelopmental disorders and epilepsy.
33410539	1	72	theme	glycosylation	238:250	arg1	disorder					226:233	a novel congenital disorder	207:233	a novel congenital disorder of glycosylation resulting in autosomal recessive inherited glycosylphosphatidylinositol-anchored protein (GPI-AP) deficiency	207:359	The phosphatidylinositol glycan anchor biosynthesis class S protein (PIGS) gene has recently been implicated in a novel congenital disorder of glycosylation resulting in autosomal recessive inherited glycosylphosphatidylinositol-anchored protein (GPI-AP) deficiency.
33410539	1	73	theme	phosphatidylinositol	99:118	arg1	anchor					127:132	The phosphatidylinositol glycan anchor biosynthesis class S protein (PIGS) gene	95:173	The phosphatidylinositol glycan anchor biosynthesis class S protein (PIGS) gene	95:173	The phosphatidylinositol glycan anchor biosynthesis class S protein (PIGS) gene has recently been implicated in a novel congenital disorder of glycosylation resulting in autosomal recessive inherited glycosylphosphatidylinositol-anchored protein (GPI-AP) deficiency.
33410539	4	74	theme	infantile	819:827	arg1	epilepsy					829:836	intractable early infantile epilepsy	801:836	intractable early infantile epilepsy	801:836	All individuals presented with hypotonia, severe global developmental delay, microcephaly, intractable early infantile epilepsy, and structural brain abnormalities.
33410539	2	75	theme	PIGS	435:438	arg1	gene					440:443	the PIGS gene	431:443	the PIGS gene	431:443	Previous studies described seven patients with biallelic variants in the PIGS gene, of whom two presented with fetal akinesia and five with global developmental delay and epileptic developmental encephalopathy.
33410539	5	76	theme	hearing	922:928	arg1	loss					930:933	hearing loss	922:933	hearing loss	922:933	Additional findings include vision impairment, hearing loss, renal malformation, and hypotonic facial appearances with minor dysmorphic features but without a distinctive facial gestalt.
33410539	2	77	theme	fetal	473:477	arg1	akinesia					479:486	fetal akinesia	473:486	fetal akinesia	473:486	Previous studies described seven patients with biallelic variants in the PIGS gene, of whom two presented with fetal akinesia and five with global developmental delay and epileptic developmental encephalopathy.
33410539	3	78	from	families	667:674	arg1	characteristics					611:625	the molecular and clinical characteristics	584:625	the molecular and clinical characteristics of six additional individuals from five families with unreported variants in PIGS	584:707	We present the molecular and clinical characteristics of six additional individuals from five families with unreported variants in PIGS.
33410539	3	78	from	families	667:674	arg1	individuals					645:655	six additional individuals	630:655	six additional individuals from five families with unreported variants in PIGS	630:707	We present the molecular and clinical characteristics of six additional individuals from five families with unreported variants in PIGS.
33410539	5	79	theme	Additional	875:884	arg1	findings					886:893	Additional findings	875:893	Additional findings	875:893	Additional findings include vision impairment, hearing loss, renal malformation, and hypotonic facial appearances with minor dysmorphic features but without a distinctive facial gestalt.
33410539	1	80	theme	congenital	215:224	arg1	disorder					226:233	a novel congenital disorder	207:233	a novel congenital disorder of glycosylation resulting in autosomal recessive inherited glycosylphosphatidylinositol-anchored protein (GPI-AP) deficiency	207:359	The phosphatidylinositol glycan anchor biosynthesis class S protein (PIGS) gene has recently been implicated in a novel congenital disorder of glycosylation resulting in autosomal recessive inherited glycosylphosphatidylinositol-anchored protein (GPI-AP) deficiency.
33410539	2	81	theme	developmental	509:521	arg1	delay					523:527	global developmental delay	502:527	global developmental delay	502:527	Previous studies described seven patients with biallelic variants in the PIGS gene, of whom two presented with fetal akinesia and five with global developmental delay and epileptic developmental encephalopathy.
33410539	8	82	dep	genotype	1346:1353	arg1	the					1342:1344	the	1342:1344	the	1342:1344	We confirm that biallelic variants in PIGS cause vitamin pyridoxine-responsive epilepsy due to inherited GPI deficiency and expand the genotype and phenotype of PIGS-related disorder.
33410539	0	83	theme	epileptic	55:63	arg1	encephalopathy					79:92	PIGS-associated early onset epileptic developmental encephalopathy	27:92	PIGS-associated early onset epileptic developmental encephalopathy	27:92	Expanding the phenotype of PIGS-associated early onset epileptic developmental encephalopathy.
33410539	2	84	with	patients	395:402	arg1	variants					419:426	biallelic variants	409:426	biallelic variants in the PIGS gene, of whom two presented with fetal akinesia	409:486	Previous studies described seven patients with biallelic variants in the PIGS gene, of whom two presented with fetal akinesia and five with global developmental delay and epileptic developmental encephalopathy.
33410539	5	85	with	appearances	977:987	arg1	features					1011:1018	minor dysmorphic features	994:1018	minor dysmorphic features	994:1018	Additional findings include vision impairment, hearing loss, renal malformation, and hypotonic facial appearances with minor dysmorphic features but without a distinctive facial gestalt.
33410539	9	86	theme	diagnostic	1549:1558	arg1	testing					1568:1574	diagnostic genetic testing	1549:1574	diagnostic genetic testing	1549:1574	Further delineation of the molecular spectrum of PIGS-related disorders would improve management, help develop treatments, and encourage the expansion of diagnostic genetic testing to include this gene as a potential cause of neurodevelopmental disorders and epilepsy.
33410539	8	87	theme	vitamin	1260:1266	arg1	epilepsy					1290:1297	vitamin pyridoxine-responsive epilepsy	1260:1297	vitamin pyridoxine-responsive epilepsy due to inherited GPI deficiency	1260:1329	We confirm that biallelic variants in PIGS cause vitamin pyridoxine-responsive epilepsy due to inherited GPI deficiency and expand the genotype and phenotype of PIGS-related disorder.
33410539	9	88	theme	testing	1568:1574	arg1	expansion					1536:1544	the expansion	1532:1544	the expansion of diagnostic genetic testing to include this gene as a potential cause of neurodevelopmental disorders and epilepsy	1532:1661	Further delineation of the molecular spectrum of PIGS-related disorders would improve management, help develop treatments, and encourage the expansion of diagnostic genetic testing to include this gene as a potential cause of neurodevelopmental disorders and epilepsy.
32985911	5	0	theme	@	766:766	arg1	emulsion					756:763	BD emulsion	753:763	BD emulsion (@BD) without PRO	753:781	BD emulsion (@BD) without PRO was produced for comparation.
32985911	5	0	theme	@	766:766	arg1	BD					767:768	@BD	766:768	@BD	766:768	BD emulsion (@BD) without PRO was produced for comparation.
32985911	9	1	theme	commercial	1270:1279	arg1	injection					1285:1293	intravenously injected commercial PTX injection	1247:1293	intravenously injected commercial PTX injection	1247:1293	More importantly, orally administrated PTX@BD/PRO had the same anti-tumor efficacy as intravenously injected commercial PTX injection.
32985911	10	2	theme	oral	1373:1376	arg1	administration					1378:1391	consecutive oral administration	1361:1391	consecutive oral administration of PTX@BD/PRO	1361:1405	No abnormality was observed in the main organs of the mice after consecutive oral administration of PTX@BD/PRO.
32985911	3	3	theme	Maillard	520:527	arg1	reaction					529:536	Maillard reaction	520:536	Maillard reaction	520:536	The electrostatic complex of PRO and BSA-dextran conjugate (BD) produced by Maillard reaction was used as emulsifier to produce oil-in-water emulsion (@BD/PRO).
32985911	10	4	theme	PTX	1396:1398	arg1	BD/PRO					1400:1405	PTX@BD/PRO	1396:1405	PTX@BD/PRO	1396:1405	No abnormality was observed in the main organs of the mice after consecutive oral administration of PTX@BD/PRO.
32985911	8	5	theme	@	1103:1103	arg1	BD					1104:1105	PTX@BD	1100:1105	PTX@BD	1100:1105	The bioavailability and H22 tumor inhibition efficacy of PTX@BD/PRO were 40% and 70% higher than those of PTX@BD, respectively, after oral administration in the mice.
32985911	4	6	theme	PRO	695:697	arg1	chains					699:704	the PRO chains	691:704	the PRO chains	691:704	The BSA molecules were crosslinked at the oil-water interface by a heat treatment and the PRO chains were simultaneously anchored in the interface.
32985911	8	7	theme	PTX	1100:1102	arg1	BD					1104:1105	PTX@BD	1100:1105	PTX@BD	1100:1105	The bioavailability and H22 tumor inhibition efficacy of PTX@BD/PRO were 40% and 70% higher than those of PTX@BD, respectively, after oral administration in the mice.
32985911	8	8	theme	H22	1018:1020	arg1	efficacy					1039:1046	H22 tumor inhibition efficacy	1018:1046	H22 tumor inhibition efficacy	1018:1046	The bioavailability and H22 tumor inhibition efficacy of PTX@BD/PRO were 40% and 70% higher than those of PTX@BD, respectively, after oral administration in the mice.
32985911	0	9	theme	paclitaxel	100:109	arg1	efficacy					88:95	anti-tumor efficacy	77:95	anti-tumor efficacy	77:95	Protamine and BSA-dextran complex emulsion improves oral bioavailability and anti-tumor efficacy of paclitaxel.
32985911	0	9	theme	paclitaxel	100:109	arg1	bioavailability					57:71	oral bioavailability	52:71	oral bioavailability	52:71	Protamine and BSA-dextran complex emulsion improves oral bioavailability and anti-tumor efficacy of paclitaxel.
32985911	9	10	theme	same	1219:1222	arg1	efficacy					1235:1242	the same anti-tumor efficacy	1215:1242	the same anti-tumor efficacy	1215:1242	More importantly, orally administrated PTX@BD/PRO had the same anti-tumor efficacy as intravenously injected commercial PTX injection.
32985911	10	11	theme	mice	1350:1353	arg1	organs					1336:1341	the main organs	1327:1341	the main organs of the mice	1327:1353	No abnormality was observed in the main organs of the mice after consecutive oral administration of PTX@BD/PRO.
32985911	10	12	located	observed	1315:1322	arg2	abnormality					1299:1309	No abnormality	1296:1309	No abnormality	1296:1309	No abnormality was observed in the main organs of the mice after consecutive oral administration of PTX@BD/PRO.
32985911	10	12	located	observed	1315:1322	arg1	organs					1336:1341	the main organs	1327:1341	the main organs of the mice	1327:1353	No abnormality was observed in the main organs of the mice after consecutive oral administration of PTX@BD/PRO.
32985911	8	13	dep	bioavailability	998:1012	arg1	The					994:996	The	994:996	The	994:996	The bioavailability and H22 tumor inhibition efficacy of PTX@BD/PRO were 40% and 70% higher than those of PTX@BD, respectively, after oral administration in the mice.
32985911	9	14	theme	anti-tumor	1224:1233	arg1	efficacy					1235:1242	the same anti-tumor efficacy	1215:1242	the same anti-tumor efficacy	1215:1242	More importantly, orally administrated PTX@BD/PRO had the same anti-tumor efficacy as intravenously injected commercial PTX injection.
32985911	8	15	theme	inhibition	1028:1037	arg1	efficacy					1039:1046	H22 tumor inhibition efficacy	1018:1046	H22 tumor inhibition efficacy	1018:1046	The bioavailability and H22 tumor inhibition efficacy of PTX@BD/PRO were 40% and 70% higher than those of PTX@BD, respectively, after oral administration in the mice.
32985911	10	16	theme	consecutive	1361:1371	arg1	administration					1378:1391	consecutive oral administration	1361:1391	consecutive oral administration of PTX@BD/PRO	1361:1405	No abnormality was observed in the main organs of the mice after consecutive oral administration of PTX@BD/PRO.
32985911	9	17	contain	had	1211:1213	arg2	efficacy					1235:1242	the same anti-tumor efficacy	1215:1242	the same anti-tumor efficacy	1215:1242	More importantly, orally administrated PTX@BD/PRO had the same anti-tumor efficacy as intravenously injected commercial PTX injection.
32985911	9	17	contain	had	1211:1213	arg1	BD/PRO					1204:1209	orally administrated PTX@BD/PRO	1179:1209	orally administrated PTX@BD/PRO	1179:1209	More importantly, orally administrated PTX@BD/PRO had the same anti-tumor efficacy as intravenously injected commercial PTX injection.
32985911	1	18	theme	hydrophobic	183:193	arg1	drugs					195:199	hydrophobic drugs	183:199	hydrophobic drugs	183:199	Food protein and polysaccharide complex emulsions are safe carriers of hydrophobic drugs and nutrients.
32985911	5	19	theme	BD	753:754	arg1	emulsion					756:763	BD emulsion	753:763	BD emulsion (@BD) without PRO	753:781	BD emulsion (@BD) without PRO was produced for comparation.
32985911	5	19	theme	BD	753:754	arg1	BD					767:768	@BD	766:768	@BD	766:768	BD emulsion (@BD) without PRO was produced for comparation.
32985911	8	20	from	administration	1133:1146	arg1	mice					1155:1158	the mice	1151:1158	the mice	1151:1158	The bioavailability and H22 tumor inhibition efficacy of PTX@BD/PRO were 40% and 70% higher than those of PTX@BD, respectively, after oral administration in the mice.
32985911	8	21	theme	tumor	1022:1026	arg1	efficacy					1039:1046	H22 tumor inhibition efficacy	1018:1046	H22 tumor inhibition efficacy	1018:1046	The bioavailability and H22 tumor inhibition efficacy of PTX@BD/PRO were 40% and 70% higher than those of PTX@BD, respectively, after oral administration in the mice.
32985911	10	22	theme	BD/PRO	1400:1405	arg1	administration					1378:1391	consecutive oral administration	1361:1391	consecutive oral administration of PTX@BD/PRO	1361:1405	No abnormality was observed in the main organs of the mice after consecutive oral administration of PTX@BD/PRO.
32985911	2	23	theme	nutrients	306:314	arg1	bioavailability					232:246	oral bioavailability	227:246	oral bioavailability	227:246	To improve oral bioavailability and therapeutic/healthy efficacy of hydrophobic drugs and nutrients, herein, protamine (PRO), a cationic cell-penetrating peptide, was introduced into protein and polysaccharide complex emulsion.
32985911	2	23	theme	nutrients	306:314	arg1	efficacy					272:279	therapeutic/healthy efficacy	252:279	therapeutic/healthy efficacy	252:279	To improve oral bioavailability and therapeutic/healthy efficacy of hydrophobic drugs and nutrients, herein, protamine (PRO), a cationic cell-penetrating peptide, was introduced into protein and polysaccharide complex emulsion.
32985911	1	24	theme	drugs	195:199	arg1	emulsions					152:160	Food protein and polysaccharide complex emulsions	112:160	Food protein and polysaccharide complex emulsions	112:160	Food protein and polysaccharide complex emulsions are safe carriers of hydrophobic drugs and nutrients.
32985911	1	24	theme	drugs	195:199	arg1	carriers					171:178	safe carriers	166:178	safe carriers of hydrophobic drugs and nutrients	166:213	Food protein and polysaccharide complex emulsions are safe carriers of hydrophobic drugs and nutrients.
32985911	9	25	theme	PTX	1281:1283	arg1	injection					1285:1293	intravenously injected commercial PTX injection	1247:1293	intravenously injected commercial PTX injection	1247:1293	More importantly, orally administrated PTX@BD/PRO had the same anti-tumor efficacy as intravenously injected commercial PTX injection.
32985911	8	26	theme	higher	1079:1084	arg1	%					1077:1077	70%	1075:1077	70% higher than those of PTX@BD	1075:1105	The bioavailability and H22 tumor inhibition efficacy of PTX@BD/PRO were 40% and 70% higher than those of PTX@BD, respectively, after oral administration in the mice.
32985911	3	27	theme	electrostatic	448:460	arg1	complex					462:468	The electrostatic complex	444:468	The electrostatic complex of PRO and BSA-dextran conjugate (BD) produced by Maillard reaction	444:536	The electrostatic complex of PRO and BSA-dextran conjugate (BD) produced by Maillard reaction was used as emulsifier to produce oil-in-water emulsion (@BD/PRO).
32985911	3	27	theme	electrostatic	448:460	arg1	emulsifier					550:559	emulsifier	550:559	emulsifier	550:559	The electrostatic complex of PRO and BSA-dextran conjugate (BD) produced by Maillard reaction was used as emulsifier to produce oil-in-water emulsion (@BD/PRO).
32985911	9	28	theme	injected	1261:1268	arg1	injection					1285:1293	intravenously injected commercial PTX injection	1247:1293	intravenously injected commercial PTX injection	1247:1293	More importantly, orally administrated PTX@BD/PRO had the same anti-tumor efficacy as intravenously injected commercial PTX injection.
32985911	6	29	theme	loading	937:943	arg1	capacity					945:952	6.4% loading capacity	932:952	6.4% loading capacity	932:952	Paclitaxel (PTX), a hydrophobic antineoplastic drug, was encapsulated in the emulsions with 99% loading efficiency and 6.4% loading capacity.
32985911	9	30	theme	PTX	1200:1202	arg1	BD/PRO					1204:1209	orally administrated PTX@BD/PRO	1179:1209	orally administrated PTX@BD/PRO	1179:1209	More importantly, orally administrated PTX@BD/PRO had the same anti-tumor efficacy as intravenously injected commercial PTX injection.
32985911	3	31	theme	@	595:595	arg1	emulsion					585:592	oil-in-water emulsion	572:592	oil-in-water emulsion (@BD/PRO)	572:602	The electrostatic complex of PRO and BSA-dextran conjugate (BD) produced by Maillard reaction was used as emulsifier to produce oil-in-water emulsion (@BD/PRO).
32985911	3	31	theme	@	595:595	arg1	BD/PRO					596:601	@BD/PRO	595:601	@BD/PRO	595:601	The electrostatic complex of PRO and BSA-dextran conjugate (BD) produced by Maillard reaction was used as emulsifier to produce oil-in-water emulsion (@BD/PRO).
32985911	1	32	theme	nutrients	205:213	arg1	emulsions					152:160	Food protein and polysaccharide complex emulsions	112:160	Food protein and polysaccharide complex emulsions	112:160	Food protein and polysaccharide complex emulsions are safe carriers of hydrophobic drugs and nutrients.
32985911	1	32	theme	nutrients	205:213	arg1	carriers					171:178	safe carriers	166:178	safe carriers of hydrophobic drugs and nutrients	166:213	Food protein and polysaccharide complex emulsions are safe carriers of hydrophobic drugs and nutrients.
32985911	0	33	theme	complex	26:32	arg1	emulsion					34:41	BSA-dextran complex emulsion	14:41	BSA-dextran complex emulsion	14:41	Protamine and BSA-dextran complex emulsion improves oral bioavailability and anti-tumor efficacy of paclitaxel.
32985911	3	34	used	used	542:545	arg2	emulsifier					550:559	emulsifier	550:559	emulsifier	550:559	The electrostatic complex of PRO and BSA-dextran conjugate (BD) produced by Maillard reaction was used as emulsifier to produce oil-in-water emulsion (@BD/PRO).
32985911	3	34	used	used	542:545	arg2	complex					462:468	The electrostatic complex	444:468	The electrostatic complex of PRO and BSA-dextran conjugate (BD) produced by Maillard reaction	444:536	The electrostatic complex of PRO and BSA-dextran conjugate (BD) produced by Maillard reaction was used as emulsifier to produce oil-in-water emulsion (@BD/PRO).
32985911	8	35	theme	@	1054:1054	arg1	BD/PRO					1055:1060	PTX@BD/PRO	1051:1060	PTX@BD/PRO	1051:1060	The bioavailability and H22 tumor inhibition efficacy of PTX@BD/PRO were 40% and 70% higher than those of PTX@BD, respectively, after oral administration in the mice.
32985911	10	36	theme	main	1331:1334	arg1	organs					1336:1341	the main organs	1327:1341	the main organs of the mice	1327:1353	No abnormality was observed in the main organs of the mice after consecutive oral administration of PTX@BD/PRO.
32985911	1	37	theme	Food	112:115	arg1	protein					117:123	Food protein	112:123	Food protein	112:123	Food protein and polysaccharide complex emulsions are safe carriers of hydrophobic drugs and nutrients.
32985911	11	38	theme	excellent	1448:1456	arg1	carrier					1458:1464	an excellent carrier	1445:1464	an excellent carrier of hydrophobic drugs/nutrients	1445:1495	This study indicates that @BD/PRO is an excellent carrier of hydrophobic drugs/nutrients and is suitable for long-term oral administration.
32985911	11	38	theme	excellent	1448:1456	arg1	BD/PRO					1435:1440	@BD/PRO	1434:1440	@BD/PRO	1434:1440	This study indicates that @BD/PRO is an excellent carrier of hydrophobic drugs/nutrients and is suitable for long-term oral administration.
32985911	3	39	theme	conjugate	493:501	arg1	complex					462:468	The electrostatic complex	444:468	The electrostatic complex of PRO and BSA-dextran conjugate (BD) produced by Maillard reaction	444:536	The electrostatic complex of PRO and BSA-dextran conjugate (BD) produced by Maillard reaction was used as emulsifier to produce oil-in-water emulsion (@BD/PRO).
32985911	3	39	theme	conjugate	493:501	arg1	emulsifier					550:559	emulsifier	550:559	emulsifier	550:559	The electrostatic complex of PRO and BSA-dextran conjugate (BD) produced by Maillard reaction was used as emulsifier to produce oil-in-water emulsion (@BD/PRO).
32985911	0	40	theme	BSA-dextran	14:24	arg1	emulsion					34:41	BSA-dextran complex emulsion	14:41	BSA-dextran complex emulsion	14:41	Protamine and BSA-dextran complex emulsion improves oral bioavailability and anti-tumor efficacy of paclitaxel.
32985911	6	41	theme	6.4	932:934	arg1	%					935:935	%	935:935	%	935:935	Paclitaxel (PTX), a hydrophobic antineoplastic drug, was encapsulated in the emulsions with 99% loading efficiency and 6.4% loading capacity.
32985911	8	42	theme	PTX	1051:1053	arg1	BD/PRO					1055:1060	PTX@BD/PRO	1051:1060	PTX@BD/PRO	1051:1060	The bioavailability and H22 tumor inhibition efficacy of PTX@BD/PRO were 40% and 70% higher than those of PTX@BD, respectively, after oral administration in the mice.
32985911	6	43	theme	antineoplastic	845:858	arg1	drug					860:863	a hydrophobic antineoplastic drug	831:863	a hydrophobic antineoplastic drug	831:863	Paclitaxel (PTX), a hydrophobic antineoplastic drug, was encapsulated in the emulsions with 99% loading efficiency and 6.4% loading capacity.
32985911	6	43	theme	antineoplastic	845:858	arg1	Paclitaxel					813:822	Paclitaxel	813:822	Paclitaxel (PTX)	813:828	Paclitaxel (PTX), a hydrophobic antineoplastic drug, was encapsulated in the emulsions with 99% loading efficiency and 6.4% loading capacity.
32985911	1	44	theme	protein	117:123	arg1	emulsions					152:160	Food protein and polysaccharide complex emulsions	112:160	Food protein and polysaccharide complex emulsions	112:160	Food protein and polysaccharide complex emulsions are safe carriers of hydrophobic drugs and nutrients.
32985911	1	44	theme	protein	117:123	arg1	carriers					171:178	safe carriers	166:178	safe carriers of hydrophobic drugs and nutrients	166:213	Food protein and polysaccharide complex emulsions are safe carriers of hydrophobic drugs and nutrients.
32985911	11	45	theme	hydrophobic	1469:1479	arg1	drugs/nutrients					1481:1495	hydrophobic drugs/nutrients	1469:1495	hydrophobic drugs/nutrients	1469:1495	This study indicates that @BD/PRO is an excellent carrier of hydrophobic drugs/nutrients and is suitable for long-term oral administration.
32985911	2	46	theme	cationic	344:351	arg1	protamine					325:333	protamine	325:333	protamine (PRO)	325:339	To improve oral bioavailability and therapeutic/healthy efficacy of hydrophobic drugs and nutrients, herein, protamine (PRO), a cationic cell-penetrating peptide, was introduced into protein and polysaccharide complex emulsion.
32985911	2	46	theme	cationic	344:351	arg1	peptide					370:376	a cationic cell-penetrating peptide	342:376	a cationic cell-penetrating peptide	342:376	To improve oral bioavailability and therapeutic/healthy efficacy of hydrophobic drugs and nutrients, herein, protamine (PRO), a cationic cell-penetrating peptide, was introduced into protein and polysaccharide complex emulsion.
32985911	6	47	theme	hydrophobic	833:843	arg1	drug					860:863	a hydrophobic antineoplastic drug	831:863	a hydrophobic antineoplastic drug	831:863	Paclitaxel (PTX), a hydrophobic antineoplastic drug, was encapsulated in the emulsions with 99% loading efficiency and 6.4% loading capacity.
32985911	6	47	theme	hydrophobic	833:843	arg1	Paclitaxel					813:822	Paclitaxel	813:822	Paclitaxel (PTX)	813:828	Paclitaxel (PTX), a hydrophobic antineoplastic drug, was encapsulated in the emulsions with 99% loading efficiency and 6.4% loading capacity.
32985911	4	48	theme	oil-water	647:655	arg1	interface					657:665	the oil-water interface	643:665	the oil-water interface	643:665	The BSA molecules were crosslinked at the oil-water interface by a heat treatment and the PRO chains were simultaneously anchored in the interface.
32985911	4	49	theme	heat	672:675	arg1	treatment					677:685	a heat treatment	670:685	a heat treatment	670:685	The BSA molecules were crosslinked at the oil-water interface by a heat treatment and the PRO chains were simultaneously anchored in the interface.
32985911	2	50	theme	polysaccharide	411:424	arg1	emulsion					434:441	polysaccharide complex emulsion	411:441	polysaccharide complex emulsion	411:441	To improve oral bioavailability and therapeutic/healthy efficacy of hydrophobic drugs and nutrients, herein, protamine (PRO), a cationic cell-penetrating peptide, was introduced into protein and polysaccharide complex emulsion.
32985911	8	51	theme	BD/PRO	1055:1060	arg1	efficacy					1039:1046	H22 tumor inhibition efficacy	1018:1046	H22 tumor inhibition efficacy	1018:1046	The bioavailability and H22 tumor inhibition efficacy of PTX@BD/PRO were 40% and 70% higher than those of PTX@BD, respectively, after oral administration in the mice.
32985911	8	51	theme	BD/PRO	1055:1060	arg1	bioavailability					998:1012	bioavailability	998:1012	bioavailability	998:1012	The bioavailability and H22 tumor inhibition efficacy of PTX@BD/PRO were 40% and 70% higher than those of PTX@BD, respectively, after oral administration in the mice.
32985911	2	52	theme	therapeutic/healthy	252:270	arg1	efficacy					272:279	therapeutic/healthy efficacy	252:279	therapeutic/healthy efficacy	252:279	To improve oral bioavailability and therapeutic/healthy efficacy of hydrophobic drugs and nutrients, herein, protamine (PRO), a cationic cell-penetrating peptide, was introduced into protein and polysaccharide complex emulsion.
32985911	2	53	theme	complex	426:432	arg1	emulsion					434:441	polysaccharide complex emulsion	411:441	polysaccharide complex emulsion	411:441	To improve oral bioavailability and therapeutic/healthy efficacy of hydrophobic drugs and nutrients, herein, protamine (PRO), a cationic cell-penetrating peptide, was introduced into protein and polysaccharide complex emulsion.
32985911	6	54	theme	loading	909:915	arg1	efficiency					917:926	99% loading efficiency	905:926	99% loading efficiency	905:926	Paclitaxel (PTX), a hydrophobic antineoplastic drug, was encapsulated in the emulsions with 99% loading efficiency and 6.4% loading capacity.
32985911	8	55	theme	oral	1128:1131	arg1	administration					1133:1146	oral administration	1128:1146	oral administration in the mice	1128:1158	The bioavailability and H22 tumor inhibition efficacy of PTX@BD/PRO were 40% and 70% higher than those of PTX@BD, respectively, after oral administration in the mice.
32985911	6	56	theme	%	935:935	arg1	capacity					945:952	6.4% loading capacity	932:952	6.4% loading capacity	932:952	Paclitaxel (PTX), a hydrophobic antineoplastic drug, was encapsulated in the emulsions with 99% loading efficiency and 6.4% loading capacity.
32985911	11	57	theme	long-term	1517:1525	arg1	administration					1532:1545	long-term oral administration	1517:1545	long-term oral administration	1517:1545	This study indicates that @BD/PRO is an excellent carrier of hydrophobic drugs/nutrients and is suitable for long-term oral administration.
32985911	2	58	theme	cell-penetrating	353:368	arg1	protamine					325:333	protamine	325:333	protamine (PRO)	325:339	To improve oral bioavailability and therapeutic/healthy efficacy of hydrophobic drugs and nutrients, herein, protamine (PRO), a cationic cell-penetrating peptide, was introduced into protein and polysaccharide complex emulsion.
32985911	2	58	theme	cell-penetrating	353:368	arg1	peptide					370:376	a cationic cell-penetrating peptide	342:376	a cationic cell-penetrating peptide	342:376	To improve oral bioavailability and therapeutic/healthy efficacy of hydrophobic drugs and nutrients, herein, protamine (PRO), a cationic cell-penetrating peptide, was introduced into protein and polysaccharide complex emulsion.
32985911	0	59	theme	oral	52:55	arg1	bioavailability					57:71	oral bioavailability	52:71	oral bioavailability	52:71	Protamine and BSA-dextran complex emulsion improves oral bioavailability and anti-tumor efficacy of paclitaxel.
32985911	6	60	theme	%	907:907	arg1	efficiency					917:926	99% loading efficiency	905:926	99% loading efficiency	905:926	Paclitaxel (PTX), a hydrophobic antineoplastic drug, was encapsulated in the emulsions with 99% loading efficiency and 6.4% loading capacity.
32985911	2	61	theme	oral	227:230	arg1	bioavailability					232:246	oral bioavailability	227:246	oral bioavailability	227:246	To improve oral bioavailability and therapeutic/healthy efficacy of hydrophobic drugs and nutrients, herein, protamine (PRO), a cationic cell-penetrating peptide, was introduced into protein and polysaccharide complex emulsion.
32985911	4	62	attach	crosslinked	628:638	arg3	interface					657:665	the oil-water interface	643:665	the oil-water interface	643:665	The BSA molecules were crosslinked at the oil-water interface by a heat treatment and the PRO chains were simultaneously anchored in the interface.
32985911	1	63	theme	polysaccharide	129:142	arg1	complex					144:150	polysaccharide complex	129:150	polysaccharide complex	129:150	Food protein and polysaccharide complex emulsions are safe carriers of hydrophobic drugs and nutrients.
32985911	6	64	theme	99	905:906	arg1	%					907:907	%	907:907	%	907:907	Paclitaxel (PTX), a hydrophobic antineoplastic drug, was encapsulated in the emulsions with 99% loading efficiency and 6.4% loading capacity.
32985911	11	65	theme	drugs/nutrients	1481:1495	arg1	carrier					1458:1464	an excellent carrier	1445:1464	an excellent carrier of hydrophobic drugs/nutrients	1445:1495	This study indicates that @BD/PRO is an excellent carrier of hydrophobic drugs/nutrients and is suitable for long-term oral administration.
32985911	11	65	theme	drugs/nutrients	1481:1495	arg1	BD/PRO					1435:1440	@BD/PRO	1434:1440	@BD/PRO	1434:1440	This study indicates that @BD/PRO is an excellent carrier of hydrophobic drugs/nutrients and is suitable for long-term oral administration.
32985911	7	66	theme	long-term	973:981	arg1	stability					983:991	long-term stability	973:991	long-term stability	973:991	The emulsions had long-term stability.
32985911	1	67	theme	complex	144:150	arg1	emulsions					152:160	Food protein and polysaccharide complex emulsions	112:160	Food protein and polysaccharide complex emulsions	112:160	Food protein and polysaccharide complex emulsions are safe carriers of hydrophobic drugs and nutrients.
32985911	1	67	theme	complex	144:150	arg1	carriers					171:178	safe carriers	166:178	safe carriers of hydrophobic drugs and nutrients	166:213	Food protein and polysaccharide complex emulsions are safe carriers of hydrophobic drugs and nutrients.
32985911	3	68	theme	oil-in-water	572:583	arg1	emulsion					585:592	oil-in-water emulsion	572:592	oil-in-water emulsion (@BD/PRO)	572:602	The electrostatic complex of PRO and BSA-dextran conjugate (BD) produced by Maillard reaction was used as emulsifier to produce oil-in-water emulsion (@BD/PRO).
32985911	3	68	theme	oil-in-water	572:583	arg1	BD/PRO					596:601	@BD/PRO	595:601	@BD/PRO	595:601	The electrostatic complex of PRO and BSA-dextran conjugate (BD) produced by Maillard reaction was used as emulsifier to produce oil-in-water emulsion (@BD/PRO).
32985911	9	69	theme	administrated	1186:1198	arg1	BD/PRO					1204:1209	orally administrated PTX@BD/PRO	1179:1209	orally administrated PTX@BD/PRO	1179:1209	More importantly, orally administrated PTX@BD/PRO had the same anti-tumor efficacy as intravenously injected commercial PTX injection.
32985911	2	70	theme	drugs	296:300	arg1	bioavailability					232:246	oral bioavailability	227:246	oral bioavailability	227:246	To improve oral bioavailability and therapeutic/healthy efficacy of hydrophobic drugs and nutrients, herein, protamine (PRO), a cationic cell-penetrating peptide, was introduced into protein and polysaccharide complex emulsion.
32985911	2	70	theme	drugs	296:300	arg1	efficacy					272:279	therapeutic/healthy efficacy	252:279	therapeutic/healthy efficacy	252:279	To improve oral bioavailability and therapeutic/healthy efficacy of hydrophobic drugs and nutrients, herein, protamine (PRO), a cationic cell-penetrating peptide, was introduced into protein and polysaccharide complex emulsion.
32985911	10	71	theme	@	1399:1399	arg1	BD/PRO					1400:1405	PTX@BD/PRO	1396:1405	PTX@BD/PRO	1396:1405	No abnormality was observed in the main organs of the mice after consecutive oral administration of PTX@BD/PRO.
32985911	6	72	with	emulsions	890:898	arg1	capacity					945:952	6.4% loading capacity	932:952	6.4% loading capacity	932:952	Paclitaxel (PTX), a hydrophobic antineoplastic drug, was encapsulated in the emulsions with 99% loading efficiency and 6.4% loading capacity.
32985911	6	72	with	emulsions	890:898	arg1	efficiency					917:926	99% loading efficiency	905:926	99% loading efficiency	905:926	Paclitaxel (PTX), a hydrophobic antineoplastic drug, was encapsulated in the emulsions with 99% loading efficiency and 6.4% loading capacity.
32985911	4	73	theme	BSA	609:611	arg1	molecules					613:621	The BSA molecules	605:621	The BSA molecules	605:621	The BSA molecules were crosslinked at the oil-water interface by a heat treatment and the PRO chains were simultaneously anchored in the interface.
32985911	0	74	theme	anti-tumor	77:86	arg1	efficacy					88:95	anti-tumor efficacy	77:95	anti-tumor efficacy	77:95	Protamine and BSA-dextran complex emulsion improves oral bioavailability and anti-tumor efficacy of paclitaxel.
32985911	3	75	theme	BSA-dextran	481:491	arg1	conjugate					493:501	BSA-dextran conjugate	481:501	BSA-dextran conjugate	481:501	The electrostatic complex of PRO and BSA-dextran conjugate (BD) produced by Maillard reaction was used as emulsifier to produce oil-in-water emulsion (@BD/PRO).
32985911	2	76	theme	hydrophobic	284:294	arg1	drugs					296:300	hydrophobic drugs	284:300	hydrophobic drugs	284:300	To improve oral bioavailability and therapeutic/healthy efficacy of hydrophobic drugs and nutrients, herein, protamine (PRO), a cationic cell-penetrating peptide, was introduced into protein and polysaccharide complex emulsion.
32985911	3	77	theme	PRO	473:475	arg1	complex					462:468	The electrostatic complex	444:468	The electrostatic complex of PRO and BSA-dextran conjugate (BD) produced by Maillard reaction	444:536	The electrostatic complex of PRO and BSA-dextran conjugate (BD) produced by Maillard reaction was used as emulsifier to produce oil-in-water emulsion (@BD/PRO).
32985911	3	77	theme	PRO	473:475	arg1	emulsifier					550:559	emulsifier	550:559	emulsifier	550:559	The electrostatic complex of PRO and BSA-dextran conjugate (BD) produced by Maillard reaction was used as emulsifier to produce oil-in-water emulsion (@BD/PRO).
32985911	9	78	theme	@	1203:1203	arg1	BD/PRO					1204:1209	orally administrated PTX@BD/PRO	1179:1209	orally administrated PTX@BD/PRO	1179:1209	More importantly, orally administrated PTX@BD/PRO had the same anti-tumor efficacy as intravenously injected commercial PTX injection.
32985911	11	79	theme	oral	1527:1530	arg1	administration					1532:1545	long-term oral administration	1517:1545	long-term oral administration	1517:1545	This study indicates that @BD/PRO is an excellent carrier of hydrophobic drugs/nutrients and is suitable for long-term oral administration.
32985911	1	80	theme	safe	166:169	arg1	emulsions					152:160	Food protein and polysaccharide complex emulsions	112:160	Food protein and polysaccharide complex emulsions	112:160	Food protein and polysaccharide complex emulsions are safe carriers of hydrophobic drugs and nutrients.
32985911	1	80	theme	safe	166:169	arg1	carriers					171:178	safe carriers	166:178	safe carriers of hydrophobic drugs and nutrients	166:213	Food protein and polysaccharide complex emulsions are safe carriers of hydrophobic drugs and nutrients.
32985911	11	81	theme	@	1434:1434	arg1	carrier					1458:1464	an excellent carrier	1445:1464	an excellent carrier of hydrophobic drugs/nutrients	1445:1495	This study indicates that @BD/PRO is an excellent carrier of hydrophobic drugs/nutrients and is suitable for long-term oral administration.
32985911	11	81	theme	@	1434:1434	arg1	BD/PRO					1435:1440	@BD/PRO	1434:1440	@BD/PRO	1434:1440	This study indicates that @BD/PRO is an excellent carrier of hydrophobic drugs/nutrients and is suitable for long-term oral administration.
32985911	7	82	contain	had	969:971	arg1	emulsions					959:967	The emulsions	955:967	The emulsions	955:967	The emulsions had long-term stability.
32985911	7	82	contain	had	969:971	arg2	stability					983:991	long-term stability	973:991	long-term stability	973:991	The emulsions had long-term stability.
34042922	0	0	theme	fecal	71:75	arg1	fermentation					77:88	in vitro fecal fermentation	62:88	in vitro fecal fermentation	62:88	Cell wall permeability of pinto bean cotyledon cells regulate in vitro fecal fermentation and gut microbiota.
34042922	6	1	theme	microbial	1199:1207	arg1	enzymes					1209:1215	microbial enzymes	1199:1215	microbial enzymes	1199:1215	With an increase in cell wall permeability, microbes and/or microbial enzymes have easier access to intracellular starch for fermentation, leading to an increase in the production of metabolites and the abundance of beneficial microbes.
34042922	1	2	theme	whole	151:155	arg1	foods					157:161	whole foods	151:161	whole foods for the regulation of the colonic fermentation rate and microbiota composition	151:240	Processing induced structural changes of whole foods for the regulation of the colonic fermentation rate and microbiota composition are least understood and often overlooked.
34042922	6	3	contain	have	1217:1220	arg1	microbes					1183:1190	microbes	1183:1190	microbes	1183:1190	With an increase in cell wall permeability, microbes and/or microbial enzymes have easier access to intracellular starch for fermentation, leading to an increase in the production of metabolites and the abundance of beneficial microbes.
34042922	6	3	contain	have	1217:1220	arg1	enzymes					1209:1215	microbial enzymes	1199:1215	microbial enzymes	1199:1215	With an increase in cell wall permeability, microbes and/or microbial enzymes have easier access to intracellular starch for fermentation, leading to an increase in the production of metabolites and the abundance of beneficial microbes.
34042922	6	3	contain	have	1217:1220	arg2	access					1229:1234	easier access	1222:1234	easier access to intracellular starch for fermentation	1222:1275	With an increase in cell wall permeability, microbes and/or microbial enzymes have easier access to intracellular starch for fermentation, leading to an increase in the production of metabolites and the abundance of beneficial microbes.
34042922	3	4	theme	fatty	780:784	arg1	SCFAs					793:797	SCFAs	793:797	SCFAs	793:797	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	3	4	theme	fatty	780:784	arg1	acids					786:790	short-chain fatty acids	768:790	short-chain fatty acids (SCFAs) from the pinto bean cells	768:824	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	7	5	theme	desired	1382:1388	arg1	profiles					1411:1418	desired colonic fermentation profiles	1382:1418	desired colonic fermentation profiles	1382:1418	Thus, desired colonic fermentation profiles can be achieved with the controlled processing of whole foods for enhanced gut health.
34042922	6	6	theme	cell	1159:1162	arg1	permeability					1169:1180	cell wall permeability	1159:1180	cell wall permeability	1159:1180	With an increase in cell wall permeability, microbes and/or microbial enzymes have easier access to intracellular starch for fermentation, leading to an increase in the production of metabolites and the abundance of beneficial microbes.
34042922	1	7	theme	foods	157:161	arg1	changes					140:146	Processing induced structural changes	110:146	Processing induced structural changes of whole foods for the regulation of the colonic fermentation rate and microbiota composition	110:240	Processing induced structural changes of whole foods for the regulation of the colonic fermentation rate and microbiota composition are least understood and often overlooked.
34042922	3	8	theme	acids	786:790	arg1	production					754:763	production	754:763	production of short-chain fatty acids (SCFAs) from the pinto bean cells	754:824	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	3	8	theme	acids	786:790	arg1	rate					745:748	the in vitro fecal fermentation rate	713:748	the in vitro fecal fermentation rate	713:748	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	3	9	theme	hydrothermal	653:664	arg1	temperature					666:676	the hydrothermal temperature	649:676	the hydrothermal temperature	649:676	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	3	10	theme	cell	517:520	arg1	function					637:644	a function	635:644	a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells	635:824	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	3	10	theme	cell	517:520	arg1	permeability					527:538	The cell wall permeability	513:538	The cell wall permeability	513:538	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	3	11	theme	pinto	809:813	arg1	cells					820:824	the pinto bean cells	805:824	the pinto bean cells	805:824	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	5	12	theme	intact	1125:1130	arg1	cells					1132:1136	intact cells	1125:1136	intact cells	1125:1136	We conclude that cell wall provides an effective barrier for the microbial fermentation of intact cells.
34042922	2	13	theme	wall	494:497	arg1	permeability					499:510	the cell wall permeability	485:510	the cell wall permeability	485:510	In the present study, intact cotyledon cells from pinto beans were isolated as a whole pulse food model and subjected to a series of processing temperatures to modulate the structure, most dominantly the cell wall permeability.
34042922	2	14	dep	modulate	445:452	arg1	permeability					499:510	the cell wall permeability	485:510	the cell wall permeability	485:510	In the present study, intact cotyledon cells from pinto beans were isolated as a whole pulse food model and subjected to a series of processing temperatures to modulate the structure, most dominantly the cell wall permeability.
34042922	5	15	theme	effective	1073:1081	arg1	barrier					1083:1089	an effective barrier	1070:1089	an effective barrier for the microbial fermentation of intact cells	1070:1136	We conclude that cell wall provides an effective barrier for the microbial fermentation of intact cells.
34042922	3	16	theme	wall	522:525	arg1	function					637:644	a function	635:644	a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells	635:824	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	3	16	theme	wall	522:525	arg1	permeability					527:538	The cell wall permeability	513:538	The cell wall permeability	513:538	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	2	17	theme	cotyledon	314:322	arg1	cells					324:328	intact cotyledon cells	307:328	intact cotyledon cells from pinto beans	307:345	In the present study, intact cotyledon cells from pinto beans were isolated as a whole pulse food model and subjected to a series of processing temperatures to modulate the structure, most dominantly the cell wall permeability.
34042922	2	17	theme	cotyledon	314:322	arg1	model					383:387	a whole pulse food model	364:387	a whole pulse food model	364:387	In the present study, intact cotyledon cells from pinto beans were isolated as a whole pulse food model and subjected to a series of processing temperatures to modulate the structure, most dominantly the cell wall permeability.
34042922	2	18	theme	cell	489:492	arg1	permeability					499:510	the cell wall permeability	485:510	the cell wall permeability	485:510	In the present study, intact cotyledon cells from pinto beans were isolated as a whole pulse food model and subjected to a series of processing temperatures to modulate the structure, most dominantly the cell wall permeability.
34042922	3	19	dep	in	717:718	arg1	vitro					720:724	vitro	720:724	vitro	720:724	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	6	20	theme	easier	1222:1227	arg1	access					1229:1234	easier access	1222:1234	easier access to intracellular starch for fermentation	1222:1275	With an increase in cell wall permeability, microbes and/or microbial enzymes have easier access to intracellular starch for fermentation, leading to an increase in the production of metabolites and the abundance of beneficial microbes.
34042922	5	21	theme	cells	1132:1136	arg1	fermentation					1109:1120	the microbial fermentation	1095:1120	the microbial fermentation of intact cells	1095:1136	We conclude that cell wall provides an effective barrier for the microbial fermentation of intact cells.
34042922	6	22	from	increase	1147:1154	arg1	permeability					1169:1180	cell wall permeability	1159:1180	cell wall permeability	1159:1180	With an increase in cell wall permeability, microbes and/or microbial enzymes have easier access to intracellular starch for fermentation, leading to an increase in the production of metabolites and the abundance of beneficial microbes.
34042922	2	23	theme	food	378:381	arg1	cells					324:328	intact cotyledon cells	307:328	intact cotyledon cells from pinto beans	307:345	In the present study, intact cotyledon cells from pinto beans were isolated as a whole pulse food model and subjected to a series of processing temperatures to modulate the structure, most dominantly the cell wall permeability.
34042922	2	23	theme	food	378:381	arg1	model					383:387	a whole pulse food model	364:387	a whole pulse food model	364:387	In the present study, intact cotyledon cells from pinto beans were isolated as a whole pulse food model and subjected to a series of processing temperatures to modulate the structure, most dominantly the cell wall permeability.
34042922	0	24	theme	gut	94:96	arg1	microbiota					98:107	gut microbiota	94:107	gut microbiota	94:107	Cell wall permeability of pinto bean cotyledon cells regulate in vitro fecal fermentation and gut microbiota.
34042922	6	25	theme	wall	1164:1167	arg1	permeability					1169:1180	cell wall permeability	1159:1180	cell wall permeability	1159:1180	With an increase in cell wall permeability, microbes and/or microbial enzymes have easier access to intracellular starch for fermentation, leading to an increase in the production of metabolites and the abundance of beneficial microbes.
34042922	3	26	from	cells	820:824	arg1	SCFAs					793:797	SCFAs	793:797	SCFAs	793:797	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	3	26	from	cells	820:824	arg1	production					754:763	production	754:763	production of short-chain fatty acids (SCFAs) from the pinto bean cells	754:824	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	3	26	from	cells	820:824	arg1	rate					745:748	the in vitro fecal fermentation rate	713:748	the in vitro fecal fermentation rate	713:748	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	3	26	from	cells	820:824	arg1	acids					786:790	short-chain fatty acids	768:790	short-chain fatty acids (SCFAs) from the pinto bean cells	768:824	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	2	27	theme	pulse	372:376	arg1	cells					324:328	intact cotyledon cells	307:328	intact cotyledon cells from pinto beans	307:345	In the present study, intact cotyledon cells from pinto beans were isolated as a whole pulse food model and subjected to a series of processing temperatures to modulate the structure, most dominantly the cell wall permeability.
34042922	2	27	theme	pulse	372:376	arg1	model					383:387	a whole pulse food model	364:387	a whole pulse food model	364:387	In the present study, intact cotyledon cells from pinto beans were isolated as a whole pulse food model and subjected to a series of processing temperatures to modulate the structure, most dominantly the cell wall permeability.
34042922	0	28	theme	wall	5:8	arg1	permeability					10:21	Cell wall permeability	0:21	Cell wall permeability of pinto bean cotyledon cells	0:51	Cell wall permeability of pinto bean cotyledon cells regulate in vitro fecal fermentation and gut microbiota.
34042922	3	29	from	rise	705:708	arg1	production					754:763	production	754:763	production of short-chain fatty acids (SCFAs) from the pinto bean cells	754:824	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	3	29	from	rise	705:708	arg1	rate					745:748	the in vitro fecal fermentation rate	713:748	the in vitro fecal fermentation rate	713:748	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	2	30	from	beans	341:345	arg1	cells					324:328	intact cotyledon cells	307:328	intact cotyledon cells from pinto beans	307:345	In the present study, intact cotyledon cells from pinto beans were isolated as a whole pulse food model and subjected to a series of processing temperatures to modulate the structure, most dominantly the cell wall permeability.
34042922	2	30	from	beans	341:345	arg1	model					383:387	a whole pulse food model	364:387	a whole pulse food model	364:387	In the present study, intact cotyledon cells from pinto beans were isolated as a whole pulse food model and subjected to a series of processing temperatures to modulate the structure, most dominantly the cell wall permeability.
34042922	2	31	theme	intact	307:312	arg1	cells					324:328	intact cotyledon cells	307:328	intact cotyledon cells from pinto beans	307:345	In the present study, intact cotyledon cells from pinto beans were isolated as a whole pulse food model and subjected to a series of processing temperatures to modulate the structure, most dominantly the cell wall permeability.
34042922	2	31	theme	intact	307:312	arg1	model					383:387	a whole pulse food model	364:387	a whole pulse food model	364:387	In the present study, intact cotyledon cells from pinto beans were isolated as a whole pulse food model and subjected to a series of processing temperatures to modulate the structure, most dominantly the cell wall permeability.
34042922	3	32	theme	short-chain	768:778	arg1	SCFAs					793:797	SCFAs	793:797	SCFAs	793:797	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	3	32	theme	short-chain	768:778	arg1	acids					786:790	short-chain fatty acids	768:790	short-chain fatty acids (SCFAs) from the pinto bean cells	768:824	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	2	33	theme	whole	366:370	arg1	cells					324:328	intact cotyledon cells	307:328	intact cotyledon cells from pinto beans	307:345	In the present study, intact cotyledon cells from pinto beans were isolated as a whole pulse food model and subjected to a series of processing temperatures to modulate the structure, most dominantly the cell wall permeability.
34042922	2	33	theme	whole	366:370	arg1	model					383:387	a whole pulse food model	364:387	a whole pulse food model	364:387	In the present study, intact cotyledon cells from pinto beans were isolated as a whole pulse food model and subjected to a series of processing temperatures to modulate the structure, most dominantly the cell wall permeability.
34042922	0	34	theme	Cell	0:3	arg1	permeability					10:21	Cell wall permeability	0:21	Cell wall permeability of pinto bean cotyledon cells	0:51	Cell wall permeability of pinto bean cotyledon cells regulate in vitro fecal fermentation and gut microbiota.
34042922	5	35	theme	cell	1051:1054	arg1	wall					1056:1059	cell wall	1051:1059	cell wall	1051:1059	We conclude that cell wall provides an effective barrier for the microbial fermentation of intact cells.
34042922	4	36	theme	°C-treated	1017:1026	arg1	ones					1028:1031	the 60 °C-treated ones	1010:1031	the 60 °C-treated ones	1010:1031	Further, the abundance of beneficial microbiota, such as Roseburia, Lachnospiraceae, Bacteroides, and Coprococcus, were significantly higher for cells processed at 100 °C compared to the 60 °C-treated ones.
34042922	6	37	from	increase	1292:1299	arg1	abundance					1342:1350	the abundance	1338:1350	the abundance of beneficial microbes	1338:1373	With an increase in cell wall permeability, microbes and/or microbial enzymes have easier access to intracellular starch for fermentation, leading to an increase in the production of metabolites and the abundance of beneficial microbes.
34042922	6	37	from	increase	1292:1299	arg1	production					1308:1317	the production	1304:1317	the production of metabolites	1304:1332	With an increase in cell wall permeability, microbes and/or microbial enzymes have easier access to intracellular starch for fermentation, leading to an increase in the production of metabolites and the abundance of beneficial microbes.
34042922	0	38	dep	in	62:63	arg1	vitro					65:69	vitro	65:69	vitro	65:69	Cell wall permeability of pinto bean cotyledon cells regulate in vitro fecal fermentation and gut microbiota.
34042922	6	39	theme	beneficial	1355:1364	arg1	microbes					1366:1373	beneficial microbes	1355:1373	beneficial microbes	1355:1373	With an increase in cell wall permeability, microbes and/or microbial enzymes have easier access to intracellular starch for fermentation, leading to an increase in the production of metabolites and the abundance of beneficial microbes.
34042922	7	40	theme	enhanced	1486:1493	arg1	health					1499:1504	enhanced gut health	1486:1504	enhanced gut health	1486:1504	Thus, desired colonic fermentation profiles can be achieved with the controlled processing of whole foods for enhanced gut health.
34042922	6	41	theme	metabolites	1322:1332	arg1	abundance					1342:1350	the abundance	1338:1350	the abundance of beneficial microbes	1338:1373	With an increase in cell wall permeability, microbes and/or microbial enzymes have easier access to intracellular starch for fermentation, leading to an increase in the production of metabolites and the abundance of beneficial microbes.
34042922	6	41	theme	metabolites	1322:1332	arg1	production					1308:1317	the production	1304:1317	the production of metabolites	1304:1332	With an increase in cell wall permeability, microbes and/or microbial enzymes have easier access to intracellular starch for fermentation, leading to an increase in the production of metabolites and the abundance of beneficial microbes.
34042922	2	42	theme	present	292:298	arg1	study					300:304	the present study	288:304	the present study	288:304	In the present study, intact cotyledon cells from pinto beans were isolated as a whole pulse food model and subjected to a series of processing temperatures to modulate the structure, most dominantly the cell wall permeability.
34042922	1	43	theme	colonic	189:195	arg1	rate					210:213	the colonic fermentation rate	185:213	the colonic fermentation rate	185:213	Processing induced structural changes of whole foods for the regulation of the colonic fermentation rate and microbiota composition are least understood and often overlooked.
34042922	0	44	theme	bean	32:35	arg1	cells					47:51	pinto bean cotyledon cells	26:51	pinto bean cotyledon cells	26:51	Cell wall permeability of pinto bean cotyledon cells regulate in vitro fecal fermentation and gut microbiota.
34042922	5	45	theme	microbial	1099:1107	arg1	fermentation					1109:1120	the microbial fermentation	1095:1120	the microbial fermentation of intact cells	1095:1136	We conclude that cell wall provides an effective barrier for the microbial fermentation of intact cells.
34042922	4	46	theme	microbiota	864:873	arg1	higher					961:966	higher	961:966	higher	961:966	Further, the abundance of beneficial microbiota, such as Roseburia, Lachnospiraceae, Bacteroides, and Coprococcus, were significantly higher for cells processed at 100 °C compared to the 60 °C-treated ones.
34042922	4	46	theme	microbiota	864:873	arg1	abundance					840:848	the abundance	836:848	the abundance of beneficial microbiota, such as Roseburia, Lachnospiraceae, Bacteroides, and Coprococcus,	836:940	Further, the abundance of beneficial microbiota, such as Roseburia, Lachnospiraceae, Bacteroides, and Coprococcus, were significantly higher for cells processed at 100 °C compared to the 60 °C-treated ones.
34042922	4	47	theme	Lachnospiraceae	895:909	arg1	higher					961:966	higher	961:966	higher	961:966	Further, the abundance of beneficial microbiota, such as Roseburia, Lachnospiraceae, Bacteroides, and Coprococcus, were significantly higher for cells processed at 100 °C compared to the 60 °C-treated ones.
34042922	4	47	theme	Lachnospiraceae	895:909	arg1	abundance					840:848	the abundance	836:848	the abundance of beneficial microbiota, such as Roseburia, Lachnospiraceae, Bacteroides, and Coprococcus,	836:940	Further, the abundance of beneficial microbiota, such as Roseburia, Lachnospiraceae, Bacteroides, and Coprococcus, were significantly higher for cells processed at 100 °C compared to the 60 °C-treated ones.
34042922	1	48	theme	fermentation	197:208	arg1	rate					210:213	the colonic fermentation rate	185:213	the colonic fermentation rate	185:213	Processing induced structural changes of whole foods for the regulation of the colonic fermentation rate and microbiota composition are least understood and often overlooked.
34042922	0	49	theme	pinto	26:30	arg1	cells					47:51	pinto bean cotyledon cells	26:51	pinto bean cotyledon cells	26:51	Cell wall permeability of pinto bean cotyledon cells regulate in vitro fecal fermentation and gut microbiota.
34042922	4	50	theme	beneficial	853:862	arg1	microbiota					864:873	beneficial microbiota	853:873	beneficial microbiota	853:873	Further, the abundance of beneficial microbiota, such as Roseburia, Lachnospiraceae, Bacteroides, and Coprococcus, were significantly higher for cells processed at 100 °C compared to the 60 °C-treated ones.
34042922	6	51	theme	intracellular	1239:1251	arg1	starch					1253:1258	intracellular starch	1239:1258	intracellular starch	1239:1258	With an increase in cell wall permeability, microbes and/or microbial enzymes have easier access to intracellular starch for fermentation, leading to an increase in the production of metabolites and the abundance of beneficial microbes.
34042922	3	52	theme	fermentation	732:743	arg1	rate					745:748	the in vitro fecal fermentation rate	713:748	the in vitro fecal fermentation rate	713:748	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	1	53	theme	rate	210:213	arg1	regulation					171:180	the regulation	167:180	the regulation of the colonic fermentation rate and microbiota composition	167:240	Processing induced structural changes of whole foods for the regulation of the colonic fermentation rate and microbiota composition are least understood and often overlooked.
34042922	0	54	theme	cells	47:51	arg1	permeability					10:21	Cell wall permeability	0:21	Cell wall permeability of pinto bean cotyledon cells	0:51	Cell wall permeability of pinto bean cotyledon cells regulate in vitro fecal fermentation and gut microbiota.
34042922	3	55	theme	fecal	726:730	arg1	rate					745:748	the in vitro fecal fermentation rate	713:748	the in vitro fecal fermentation rate	713:748	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	7	56	theme	controlled	1445:1454	arg1	processing					1456:1465	the controlled processing	1441:1465	the controlled processing of whole foods for enhanced gut health	1441:1504	Thus, desired colonic fermentation profiles can be achieved with the controlled processing of whole foods for enhanced gut health.
34042922	7	57	theme	foods	1476:1480	arg1	processing					1456:1465	the controlled processing	1441:1465	the controlled processing of whole foods for enhanced gut health	1441:1504	Thus, desired colonic fermentation profiles can be achieved with the controlled processing of whole foods for enhanced gut health.
34042922	2	58	theme	pinto	335:339	arg1	beans					341:345	pinto beans	335:345	pinto beans	335:345	In the present study, intact cotyledon cells from pinto beans were isolated as a whole pulse food model and subjected to a series of processing temperatures to modulate the structure, most dominantly the cell wall permeability.
34042922	0	59	theme	cotyledon	37:45	arg1	cells					47:51	pinto bean cotyledon cells	26:51	pinto bean cotyledon cells	26:51	Cell wall permeability of pinto bean cotyledon cells regulate in vitro fecal fermentation and gut microbiota.
34042922	3	60	from	rate	745:748	arg1	cells					820:824	the pinto bean cells	805:824	the pinto bean cells	805:824	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	2	61	theme	temperatures	429:440	arg1	series					408:413	a series	406:413	a series of processing temperatures	406:440	In the present study, intact cotyledon cells from pinto beans were isolated as a whole pulse food model and subjected to a series of processing temperatures to modulate the structure, most dominantly the cell wall permeability.
34042922	1	62	theme	Processing	110:119	arg1	changes					140:146	Processing induced structural changes	110:146	Processing induced structural changes of whole foods for the regulation of the colonic fermentation rate and microbiota composition	110:240	Processing induced structural changes of whole foods for the regulation of the colonic fermentation rate and microbiota composition are least understood and often overlooked.
34042922	7	63	theme	colonic	1390:1396	arg1	profiles					1411:1418	desired colonic fermentation profiles	1382:1418	desired colonic fermentation profiles	1382:1418	Thus, desired colonic fermentation profiles can be achieved with the controlled processing of whole foods for enhanced gut health.
34042922	3	64	theme	in	717:718	arg1	rate					745:748	the in vitro fecal fermentation rate	713:748	the in vitro fecal fermentation rate	713:748	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	7	65	theme	gut	1495:1497	arg1	health					1499:1504	enhanced gut health	1486:1504	enhanced gut health	1486:1504	Thus, desired colonic fermentation profiles can be achieved with the controlled processing of whole foods for enhanced gut health.
34042922	1	66	theme	induced	121:127	arg1	changes					140:146	Processing induced structural changes	110:146	Processing induced structural changes of whole foods for the regulation of the colonic fermentation rate and microbiota composition	110:240	Processing induced structural changes of whole foods for the regulation of the colonic fermentation rate and microbiota composition are least understood and often overlooked.
34042922	7	67	theme	fermentation	1398:1409	arg1	profiles					1411:1418	desired colonic fermentation profiles	1382:1418	desired colonic fermentation profiles	1382:1418	Thus, desired colonic fermentation profiles can be achieved with the controlled processing of whole foods for enhanced gut health.
34042922	3	68	from	production	754:763	arg1	cells					820:824	the pinto bean cells	805:824	the pinto bean cells	805:824	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	1	69	theme	structural	129:138	arg1	changes					140:146	Processing induced structural changes	110:146	Processing induced structural changes of whole foods for the regulation of the colonic fermentation rate and microbiota composition	110:240	Processing induced structural changes of whole foods for the regulation of the colonic fermentation rate and microbiota composition are least understood and often overlooked.
34042922	4	70	dep	such	876:879	arg1	as					881:882	as	881:882	as	881:882	Further, the abundance of beneficial microbiota, such as Roseburia, Lachnospiraceae, Bacteroides, and Coprococcus, were significantly higher for cells processed at 100 °C compared to the 60 °C-treated ones.
34042922	1	71	theme	microbiota	219:228	arg1	composition					230:240	microbiota composition	219:240	microbiota composition	219:240	Processing induced structural changes of whole foods for the regulation of the colonic fermentation rate and microbiota composition are least understood and often overlooked.
34042922	0	72	theme	in	62:63	arg1	fermentation					77:88	in vitro fecal fermentation	62:88	in vitro fecal fermentation	62:88	Cell wall permeability of pinto bean cotyledon cells regulate in vitro fecal fermentation and gut microbiota.
34042922	6	73	theme	microbes	1366:1373	arg1	abundance					1342:1350	the abundance	1338:1350	the abundance of beneficial microbes	1338:1373	With an increase in cell wall permeability, microbes and/or microbial enzymes have easier access to intracellular starch for fermentation, leading to an increase in the production of metabolites and the abundance of beneficial microbes.
34042922	6	73	theme	microbes	1366:1373	arg1	production					1308:1317	the production	1304:1317	the production of metabolites	1304:1332	With an increase in cell wall permeability, microbes and/or microbial enzymes have easier access to intracellular starch for fermentation, leading to an increase in the production of metabolites and the abundance of beneficial microbes.
34042922	3	74	theme	labeled	586:592	arg1	dextran					594:600	dextran	594:600	fluorescently labeled dextran (FITC-dextran)	572:615	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	3	74	theme	labeled	586:592	arg1	FITC-dextran					603:614	FITC-dextran	603:614	FITC-dextran	603:614	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	3	75	with	line	691:694	arg1	rise					705:708	the rise	701:708	the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells	701:824	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	1	76	theme	composition	230:240	arg1	regulation					171:180	the regulation	167:180	the regulation of the colonic fermentation rate and microbiota composition	167:240	Processing induced structural changes of whole foods for the regulation of the colonic fermentation rate and microbiota composition are least understood and often overlooked.
34042922	7	77	theme	whole	1470:1474	arg1	foods					1476:1480	whole foods	1470:1480	whole foods for enhanced gut health	1470:1504	Thus, desired colonic fermentation profiles can be achieved with the controlled processing of whole foods for enhanced gut health.
34042922	3	78	theme	temperature	666:676	arg1	function					637:644	a function	635:644	a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells	635:824	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	3	78	theme	temperature	666:676	arg1	permeability					527:538	The cell wall permeability	513:538	The cell wall permeability	513:538	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	3	79	theme	dextran	594:600	arg1	diffusion					559:567	the diffusion	555:567	the diffusion of fluorescently labeled dextran (FITC-dextran)	555:615	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
34042922	2	80	theme	processing	418:427	arg1	temperatures					429:440	processing temperatures	418:440	processing temperatures	418:440	In the present study, intact cotyledon cells from pinto beans were isolated as a whole pulse food model and subjected to a series of processing temperatures to modulate the structure, most dominantly the cell wall permeability.
34042922	3	81	theme	bean	815:818	arg1	cells					820:824	the pinto bean cells	805:824	the pinto bean cells	805:824	The cell wall permeability, observed with the diffusion of fluorescently labeled dextran (FITC-dextran), was increased as a function of the hydrothermal temperature, which is in line with the rise in the in vitro fecal fermentation rate and production of short-chain fatty acids (SCFAs) from the pinto bean cells.
33050458	4	0	theme	cinnamic	921:928	arg1	pinobanksin					1088:1098	4'-methoxy pinobanksin	1077:1098	4'-methoxy pinobanksin	1077:1098	The results showed that a total of 24 compounds belonging to cinnamic acids and flavonoids, including 3,5-diisopentenyl-4-hydroxycinnamic acid (artepillin C), kaempferide, 3-isoprenyl p-coumaric acid, pinocembrin and 4'-methoxy pinobanksin, were identified in EEP-B.
33050458	4	0	theme	cinnamic	921:928	arg1	acid					1055:1058	3-isoprenyl p-coumaric acid	1032:1058	3-isoprenyl p-coumaric acid	1032:1058	The results showed that a total of 24 compounds belonging to cinnamic acids and flavonoids, including 3,5-diisopentenyl-4-hydroxycinnamic acid (artepillin C), kaempferide, 3-isoprenyl p-coumaric acid, pinocembrin and 4'-methoxy pinobanksin, were identified in EEP-B.
33050458	4	0	theme	cinnamic	921:928	arg1	pinocembrin					1061:1071	pinocembrin	1061:1071	pinocembrin	1061:1071	The results showed that a total of 24 compounds belonging to cinnamic acids and flavonoids, including 3,5-diisopentenyl-4-hydroxycinnamic acid (artepillin C), kaempferide, 3-isoprenyl p-coumaric acid, pinocembrin and 4'-methoxy pinobanksin, were identified in EEP-B.
33050458	4	0	theme	cinnamic	921:928	arg1	acid					998:1001	3,5-diisopentenyl-4-hydroxycinnamic acid	962:1001	3,5-diisopentenyl-4-hydroxycinnamic acid (artepillin C)	962:1016	The results showed that a total of 24 compounds belonging to cinnamic acids and flavonoids, including 3,5-diisopentenyl-4-hydroxycinnamic acid (artepillin C), kaempferide, 3-isoprenyl p-coumaric acid, pinocembrin and 4'-methoxy pinobanksin, were identified in EEP-B.
33050458	4	0	theme	cinnamic	921:928	arg1	kaempferide					1019:1029	kaempferide	1019:1029	kaempferide	1019:1029	The results showed that a total of 24 compounds belonging to cinnamic acids and flavonoids, including 3,5-diisopentenyl-4-hydroxycinnamic acid (artepillin C), kaempferide, 3-isoprenyl p-coumaric acid, pinocembrin and 4'-methoxy pinobanksin, were identified in EEP-B.
33050458	4	0	theme	cinnamic	921:928	arg1	acids					930:934	cinnamic acids	921:934	cinnamic acids	921:934	The results showed that a total of 24 compounds belonging to cinnamic acids and flavonoids, including 3,5-diisopentenyl-4-hydroxycinnamic acid (artepillin C), kaempferide, 3-isoprenyl p-coumaric acid, pinocembrin and 4'-methoxy pinobanksin, were identified in EEP-B.
33050458	0	1	from	Composition	13:23	arg1	Cells					108:112	Mouse Aortic Endothelial Cells	83:112	Mouse Aortic Endothelial Cells against Inflammatory Injury	83:140	The Chemical Composition of Brazilian Green Propolis and Its Protective Effects on Mouse Aortic Endothelial Cells against Inflammatory Injury.
33050458	2	2	theme	Cell	584:587	arg1	Kit-8					598:602	Cell Counting Kit-8	584:602	Cell Counting Kit-8 (CCK-8) assays	584:617	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	2	2	theme	Cell	584:587	arg1	CCK-8					605:609	CCK-8	605:609	CCK-8	605:609	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	6	3	theme	μg/mL	1364:1368	arg1	EEP-B					1370:1374	5, 10 and 20 μg/mL EEP-B	1351:1374	5, 10 and 20 μg/mL EEP-B	1351:1374	The LPS-induced levels of TNF-α, IL-6, ICAM-1, VCAM-1 and MCP-1 were downregulated in response to 5, 10 and 20 μg/mL EEP-B.
33050458	4	4	theme	artepillin	1004:1013	arg1	acid					998:1001	3,5-diisopentenyl-4-hydroxycinnamic acid	962:1001	3,5-diisopentenyl-4-hydroxycinnamic acid (artepillin C)	962:1016	The results showed that a total of 24 compounds belonging to cinnamic acids and flavonoids, including 3,5-diisopentenyl-4-hydroxycinnamic acid (artepillin C), kaempferide, 3-isoprenyl p-coumaric acid, pinocembrin and 4'-methoxy pinobanksin, were identified in EEP-B.
33050458	4	4	theme	artepillin	1004:1013	arg1	C					1015:1015	artepillin C	1004:1015	artepillin C	1004:1015	The results showed that a total of 24 compounds belonging to cinnamic acids and flavonoids, including 3,5-diisopentenyl-4-hydroxycinnamic acid (artepillin C), kaempferide, 3-isoprenyl p-coumaric acid, pinocembrin and 4'-methoxy pinobanksin, were identified in EEP-B.
33050458	5	5	theme	new	1141:1143	arg1	component					1145:1153	a new component	1139:1153	a new component	1139:1153	Among them, a new component, suggested to be 5-isoprenyl caffeic acid p-coumaric acid ester, was reported for the first time.
33050458	3	6	theme	cytokines	655:663	arg1	levels					632:637	The protein levels	620:637	The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6)	620:722	The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were analysed by western blotting.
33050458	2	7	theme	effect	350:355	arg1	extracts					284:291	ethanol extracts	276:291	ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs)	276:397	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	3	8	theme	MCP-1	807:811	arg1	expressions					813:823	ICAM-1, VCAM-1 and MCP-1 expressions	788:823	ICAM-1, VCAM-1 and MCP-1 expressions	788:823	The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were analysed by western blotting.
33050458	0	9	theme	Aortic	89:94	arg1	Cells					108:112	Mouse Aortic Endothelial Cells	83:112	Mouse Aortic Endothelial Cells against Inflammatory Injury	83:140	The Chemical Composition of Brazilian Green Propolis and Its Protective Effects on Mouse Aortic Endothelial Cells against Inflammatory Injury.
33050458	3	10	theme	tumour	665:670	arg1	TNF-α					691:695	TNF-α	691:695	TNF-α	691:695	The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were analysed by western blotting.
33050458	3	10	theme	tumour	665:670	arg1	cytokines					655:663	inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6)	642:722	inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6)	642:722	The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were analysed by western blotting.
33050458	3	10	theme	tumour	665:670	arg1	factor-α					681:688	tumour necrosis factor-α	665:688	tumour necrosis factor-α (TNF-α)	665:696	The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were analysed by western blotting.
33050458	5	11	theme	p-coumaric	1197:1206	arg1	ester					1213:1217	5-isoprenyl caffeic acid p-coumaric acid ester	1172:1217	5-isoprenyl caffeic acid p-coumaric acid ester	1172:1217	Among them, a new component, suggested to be 5-isoprenyl caffeic acid p-coumaric acid ester, was reported for the first time.
33050458	2	12	theme	Brazilian	296:304	arg1	propolis					312:319	Brazilian green propolis	296:319	Brazilian green propolis (EEP-B)	296:327	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	2	12	theme	Brazilian	296:304	arg1	EEP-B					322:326	EEP-B	322:326	EEP-B	322:326	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	8	13	theme	theoretical	1580:1590	arg1	basis					1592:1596	a theoretical basis	1578:1596	a theoretical basis for MAEC treatment using EEP-B	1578:1627	These findings could provide a theoretical basis for MAEC treatment using EEP-B.
33050458	0	14	theme	Mouse	83:87	arg1	Cells					108:112	Mouse Aortic Endothelial Cells	83:112	Mouse Aortic Endothelial Cells against Inflammatory Injury	83:140	The Chemical Composition of Brazilian Green Propolis and Its Protective Effects on Mouse Aortic Endothelial Cells against Inflammatory Injury.
33050458	7	15	theme	LPS-induced	1421:1431	arg1	reactions					1446:1454	LPS-induced inflammatory reactions	1421:1454	LPS-induced inflammatory reactions	1421:1454	This study revealed that EEP-B could reduce LPS-induced inflammatory reactions, improve cell survival, and protect MAECs by regulating ICAM-1, VCAM-1 and MCP-1 expression.
33050458	4	16	theme	3,5-diisopentenyl-4-hydroxycinnamic	962:996	arg1	acid					998:1001	3,5-diisopentenyl-4-hydroxycinnamic acid	962:1001	3,5-diisopentenyl-4-hydroxycinnamic acid (artepillin C)	962:1016	The results showed that a total of 24 compounds belonging to cinnamic acids and flavonoids, including 3,5-diisopentenyl-4-hydroxycinnamic acid (artepillin C), kaempferide, 3-isoprenyl p-coumaric acid, pinocembrin and 4'-methoxy pinobanksin, were identified in EEP-B.
33050458	4	16	theme	3,5-diisopentenyl-4-hydroxycinnamic	962:996	arg1	C					1015:1015	artepillin C	1004:1015	artepillin C	1004:1015	The results showed that a total of 24 compounds belonging to cinnamic acids and flavonoids, including 3,5-diisopentenyl-4-hydroxycinnamic acid (artepillin C), kaempferide, 3-isoprenyl p-coumaric acid, pinocembrin and 4'-methoxy pinobanksin, were identified in EEP-B.
33050458	8	17	theme	MAEC	1602:1605	arg1	treatment					1607:1615	MAEC treatment	1602:1615	MAEC treatment using EEP-B	1602:1627	These findings could provide a theoretical basis for MAEC treatment using EEP-B.
33050458	2	18	theme	EEP-B	497:501	arg1	effect					487:492	the protective effect	472:492	the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs	472:564	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	4	19	theme	p-coumaric	1044:1053	arg1	acid					1055:1058	3-isoprenyl p-coumaric acid	1032:1058	3-isoprenyl p-coumaric acid	1032:1058	The results showed that a total of 24 compounds belonging to cinnamic acids and flavonoids, including 3,5-diisopentenyl-4-hydroxycinnamic acid (artepillin C), kaempferide, 3-isoprenyl p-coumaric acid, pinocembrin and 4'-methoxy pinobanksin, were identified in EEP-B.
33050458	5	20	theme	acid	1192:1195	arg1	ester					1213:1217	5-isoprenyl caffeic acid p-coumaric acid ester	1172:1217	5-isoprenyl caffeic acid p-coumaric acid ester	1172:1217	Among them, a new component, suggested to be 5-isoprenyl caffeic acid p-coumaric acid ester, was reported for the first time.
33050458	2	21	theme	extracts	284:291	arg1	composition					261:271	the composition	257:271	the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs)	257:397	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	4	22	theme	4'-methoxy	1077:1086	arg1	pinobanksin					1088:1098	4'-methoxy pinobanksin	1077:1098	4'-methoxy pinobanksin	1077:1098	The results showed that a total of 24 compounds belonging to cinnamic acids and flavonoids, including 3,5-diisopentenyl-4-hydroxycinnamic acid (artepillin C), kaempferide, 3-isoprenyl p-coumaric acid, pinocembrin and 4'-methoxy pinobanksin, were identified in EEP-B.
33050458	1	23	theme	complex	163:169	arg1	composition					171:181	a very complex composition	156:181	a very complex composition	156:181	Propolis has a very complex composition, with antibacterial, anti-inflammatory and other properties.
33050458	0	24	theme	Endothelial	96:106	arg1	Cells					108:112	Mouse Aortic Endothelial Cells	83:112	Mouse Aortic Endothelial Cells against Inflammatory Injury	83:140	The Chemical Composition of Brazilian Green Propolis and Its Protective Effects on Mouse Aortic Endothelial Cells against Inflammatory Injury.
33050458	1	25	contain	has	152:154	arg2	composition					171:181	a very complex composition	156:181	a very complex composition	156:181	Propolis has a very complex composition, with antibacterial, anti-inflammatory and other properties.
33050458	1	25	contain	has	152:154	arg1	Propolis					143:150	Propolis	143:150	Propolis	143:150	Propolis has a very complex composition, with antibacterial, anti-inflammatory and other properties.
33050458	7	26	theme	cell	1465:1468	arg1	survival					1470:1477	cell survival	1465:1477	cell survival	1465:1477	This study revealed that EEP-B could reduce LPS-induced inflammatory reactions, improve cell survival, and protect MAECs by regulating ICAM-1, VCAM-1 and MCP-1 expression.
33050458	3	27	link	enzyme-linked	741:753	arg1	assay					769:773	enzyme-linked immunosorbent assay	741:773	enzyme-linked immunosorbent assay (ELISA)	741:781	The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were analysed by western blotting.
33050458	3	27	link	enzyme-linked	741:753	arg1	ELISA					776:780	ELISA	776:780	ELISA	776:780	The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were analysed by western blotting.
33050458	2	28	theme	ethanol	276:282	arg1	extracts					284:291	ethanol extracts	276:291	ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs)	276:397	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	0	29	theme	Chemical	4:11	arg1	Composition					13:23	The Chemical Composition	0:23	The Chemical Composition of Brazilian Green Propolis	0:51	The Chemical Composition of Brazilian Green Propolis and Its Protective Effects on Mouse Aortic Endothelial Cells against Inflammatory Injury.
33050458	2	30	theme	Kit-8	598:602	arg1	assays					612:617	Cell Counting Kit-8 (CCK-8) assays	584:617	Cell Counting Kit-8 (CCK-8) assays	584:617	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	2	31	from	effect	350:355	arg1	MAECs					392:396	MAECs	392:396	MAECs	392:396	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	2	31	from	effect	350:355	arg1	cells					385:389	mouse aortic endothelial cells	360:389	mouse aortic endothelial cells (MAECs)	360:397	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	3	32	theme	western	842:848	arg1	blotting					850:857	western blotting	842:857	western blotting	842:857	The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were analysed by western blotting.
33050458	2	33	theme	EEP-B	428:432	arg1	composition					413:423	the chemical composition	400:423	the chemical composition of EEP-B	400:432	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	3	34	dep	cytokines	655:663	arg1	TNF-α					691:695	TNF-α	691:695	TNF-α	691:695	The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were analysed by western blotting.
33050458	3	34	dep	cytokines	655:663	arg1	interleukin-					702:713	interleukin- 6	702:715	interleukin- 6 (IL-6)	702:722	The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were analysed by western blotting.
33050458	3	34	dep	cytokines	655:663	arg1	factor-α					681:688	tumour necrosis factor-α	665:688	tumour necrosis factor-α (TNF-α)	665:696	The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were analysed by western blotting.
33050458	3	34	dep	cytokines	655:663	arg1	cytokines					655:663	inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6)	642:722	inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6)	642:722	The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were analysed by western blotting.
33050458	3	34	dep	cytokines	655:663	arg1	IL-6					718:721	IL-6	718:721	IL-6	718:721	The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were analysed by western blotting.
33050458	2	35	theme	Counting	589:596	arg1	Kit-8					598:602	Cell Counting Kit-8	584:602	Cell Counting Kit-8 (CCK-8) assays	584:617	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	2	35	theme	Counting	589:596	arg1	CCK-8					605:609	CCK-8	605:609	CCK-8	605:609	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	2	36	theme	endothelial	373:383	arg1	MAECs					392:396	MAECs	392:396	MAECs	392:396	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	2	36	theme	endothelial	373:383	arg1	cells					385:389	mouse aortic endothelial cells	360:389	mouse aortic endothelial cells (MAECs)	360:397	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	7	37	theme	MCP-1	1531:1535	arg1	expression					1537:1546	ICAM-1, VCAM-1 and MCP-1 expression	1512:1546	ICAM-1, VCAM-1 and MCP-1 expression	1512:1546	This study revealed that EEP-B could reduce LPS-induced inflammatory reactions, improve cell survival, and protect MAECs by regulating ICAM-1, VCAM-1 and MCP-1 expression.
33050458	0	38	theme	Inflammatory	122:133	arg1	Injury					135:140	Inflammatory Injury	122:140	Inflammatory Injury	122:140	The Chemical Composition of Brazilian Green Propolis and Its Protective Effects on Mouse Aortic Endothelial Cells against Inflammatory Injury.
33050458	2	39	theme	propolis	312:319	arg1	extracts					284:291	ethanol extracts	276:291	ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs)	276:397	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	5	40	theme	first	1241:1245	arg1	time					1247:1250	the first time	1237:1250	the first time	1237:1250	Among them, a new component, suggested to be 5-isoprenyl caffeic acid p-coumaric acid ester, was reported for the first time.
33050458	1	41	theme	antibacterial	189:201	arg1	properties					232:241	antibacterial, anti-inflammatory and other properties	189:241	antibacterial, anti-inflammatory and other properties	189:241	Propolis has a very complex composition, with antibacterial, anti-inflammatory and other properties.
33050458	7	42	theme	VCAM-1	1520:1525	arg1	expression					1537:1546	ICAM-1, VCAM-1 and MCP-1 expression	1512:1546	ICAM-1, VCAM-1 and MCP-1 expression	1512:1546	This study revealed that EEP-B could reduce LPS-induced inflammatory reactions, improve cell survival, and protect MAECs by regulating ICAM-1, VCAM-1 and MCP-1 expression.
33050458	7	43	theme	inflammatory	1433:1444	arg1	reactions					1446:1454	LPS-induced inflammatory reactions	1421:1454	LPS-induced inflammatory reactions	1421:1454	This study revealed that EEP-B could reduce LPS-induced inflammatory reactions, improve cell survival, and protect MAECs by regulating ICAM-1, VCAM-1 and MCP-1 expression.
33050458	2	44	theme	green	306:310	arg1	propolis					312:319	Brazilian green propolis	296:319	Brazilian green propolis (EEP-B)	296:327	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	2	44	theme	green	306:310	arg1	EEP-B					322:326	EEP-B	322:326	EEP-B	322:326	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	5	45	theme	acid	1208:1211	arg1	ester					1213:1217	5-isoprenyl caffeic acid p-coumaric acid ester	1172:1217	5-isoprenyl caffeic acid p-coumaric acid ester	1172:1217	Among them, a new component, suggested to be 5-isoprenyl caffeic acid p-coumaric acid ester, was reported for the first time.
33050458	0	46	theme	Green	38:42	arg1	Propolis					44:51	Brazilian Green Propolis	28:51	Brazilian Green Propolis	28:51	The Chemical Composition of Brazilian Green Propolis and Its Protective Effects on Mouse Aortic Endothelial Cells against Inflammatory Injury.
33050458	6	47	theme	LPS-induced	1257:1267	arg1	levels					1269:1274	The LPS-induced levels	1253:1274	The LPS-induced levels of TNF-α, IL-6, ICAM-1, VCAM-1 and MCP-1	1253:1315	The LPS-induced levels of TNF-α, IL-6, ICAM-1, VCAM-1 and MCP-1 were downregulated in response to 5, 10 and 20 μg/mL EEP-B.
33050458	0	48	from	Effects	72:78	arg1	Cells					108:112	Mouse Aortic Endothelial Cells	83:112	Mouse Aortic Endothelial Cells against Inflammatory Injury	83:140	The Chemical Composition of Brazilian Green Propolis and Its Protective Effects on Mouse Aortic Endothelial Cells against Inflammatory Injury.
33050458	1	49	theme	anti-inflammatory	204:220	arg1	properties					232:241	antibacterial, anti-inflammatory and other properties	189:241	antibacterial, anti-inflammatory and other properties	189:241	Propolis has a very complex composition, with antibacterial, anti-inflammatory and other properties.
33050458	0	50	theme	Brazilian	28:36	arg1	Propolis					44:51	Brazilian Green Propolis	28:51	Brazilian Green Propolis	28:51	The Chemical Composition of Brazilian Green Propolis and Its Protective Effects on Mouse Aortic Endothelial Cells against Inflammatory Injury.
33050458	7	51	theme	ICAM-1	1512:1517	arg1	expression					1537:1546	ICAM-1, VCAM-1 and MCP-1 expression	1512:1546	ICAM-1, VCAM-1 and MCP-1 expression	1512:1546	This study revealed that EEP-B could reduce LPS-induced inflammatory reactions, improve cell survival, and protect MAECs by regulating ICAM-1, VCAM-1 and MCP-1 expression.
33050458	2	52	from	effect	487:492	arg1	proliferation					510:522	the proliferation	506:522	the proliferation of lipopolysaccharide (LPS)-induced MAECs	506:564	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	5	53	theme	5-isoprenyl	1172:1182	arg1	ester					1213:1217	5-isoprenyl caffeic acid p-coumaric acid ester	1172:1217	5-isoprenyl caffeic acid p-coumaric acid ester	1172:1217	Among them, a new component, suggested to be 5-isoprenyl caffeic acid p-coumaric acid ester, was reported for the first time.
33050458	2	54	from	cells	385:389	arg1	extracts					284:291	ethanol extracts	276:291	ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs)	276:397	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	6	55	theme	TNF-α	1279:1283	arg1	levels					1269:1274	The LPS-induced levels	1253:1274	The LPS-induced levels of TNF-α, IL-6, ICAM-1, VCAM-1 and MCP-1	1253:1315	The LPS-induced levels of TNF-α, IL-6, ICAM-1, VCAM-1 and MCP-1 were downregulated in response to 5, 10 and 20 μg/mL EEP-B.
33050458	2	56	theme	MAECs	560:564	arg1	proliferation					510:522	the proliferation	506:522	the proliferation of lipopolysaccharide (LPS)-induced MAECs	506:564	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	4	57	theme	compounds	898:906	arg1	total					886:890	a total	884:890	a total of 24 compounds belonging to cinnamic acids and flavonoids, including 3,5-diisopentenyl-4-hydroxycinnamic acid (artepillin C), kaempferide, 3-isoprenyl p-coumaric acid, pinocembrin and 4'-methoxy pinobanksin,	884:1099	The results showed that a total of 24 compounds belonging to cinnamic acids and flavonoids, including 3,5-diisopentenyl-4-hydroxycinnamic acid (artepillin C), kaempferide, 3-isoprenyl p-coumaric acid, pinocembrin and 4'-methoxy pinobanksin, were identified in EEP-B.
33050458	2	58	theme	protective	339:348	arg1	effect					350:355	their protective effect	333:355	their protective effect on mouse aortic endothelial cells (MAECs)	333:397	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	0	59	theme	Propolis	44:51	arg1	Composition					13:23	The Chemical Composition	0:23	The Chemical Composition of Brazilian Green Propolis	0:51	The Chemical Composition of Brazilian Green Propolis and Its Protective Effects on Mouse Aortic Endothelial Cells against Inflammatory Injury.
33050458	0	59	theme	Propolis	44:51	arg1	Effects					72:78	Its Protective Effects	57:78	Its Protective Effects on Mouse Aortic Endothelial Cells against Inflammatory Injury	57:140	The Chemical Composition of Brazilian Green Propolis and Its Protective Effects on Mouse Aortic Endothelial Cells against Inflammatory Injury.
33050458	2	60	theme	-induced	551:558	arg1	MAECs					560:564	lipopolysaccharide (LPS)-induced MAECs	527:564	lipopolysaccharide (LPS)-induced MAECs	527:564	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	3	61	theme	inflammatory	642:653	arg1	cytokines					655:663	inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6)	642:722	inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6)	642:722	The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were analysed by western blotting.
33050458	3	61	theme	inflammatory	642:653	arg1	factor-α					681:688	tumour necrosis factor-α	665:688	tumour necrosis factor-α (TNF-α)	665:696	The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were analysed by western blotting.
33050458	3	61	theme	inflammatory	642:653	arg1	interleukin-					702:713	interleukin- 6	702:715	interleukin- 6 (IL-6)	702:722	The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were analysed by western blotting.
33050458	2	62	from	extracts	284:291	arg1	MAECs					392:396	MAECs	392:396	MAECs	392:396	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	2	62	from	extracts	284:291	arg1	cells					385:389	mouse aortic endothelial cells	360:389	mouse aortic endothelial cells (MAECs)	360:397	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	3	63	theme	VCAM-1	796:801	arg1	expressions					813:823	ICAM-1, VCAM-1 and MCP-1 expressions	788:823	ICAM-1, VCAM-1 and MCP-1 expressions	788:823	The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were analysed by western blotting.
33050458	1	64	theme	other	226:230	arg1	properties					232:241	antibacterial, anti-inflammatory and other properties	189:241	antibacterial, anti-inflammatory and other properties	189:241	Propolis has a very complex composition, with antibacterial, anti-inflammatory and other properties.
33050458	1	65	with	composition	171:181	arg1	properties					232:241	antibacterial, anti-inflammatory and other properties	189:241	antibacterial, anti-inflammatory and other properties	189:241	Propolis has a very complex composition, with antibacterial, anti-inflammatory and other properties.
33050458	3	66	theme	protein	624:630	arg1	levels					632:637	The protein levels	620:637	The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6)	620:722	The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were analysed by western blotting.
33050458	3	67	theme	ICAM-1	788:793	arg1	expressions					813:823	ICAM-1, VCAM-1 and MCP-1 expressions	788:823	ICAM-1, VCAM-1 and MCP-1 expressions	788:823	The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were analysed by western blotting.
33050458	0	68	theme	Protective	61:70	arg1	Effects					72:78	Its Protective Effects	57:78	Its Protective Effects on Mouse Aortic Endothelial Cells against Inflammatory Injury	57:140	The Chemical Composition of Brazilian Green Propolis and Its Protective Effects on Mouse Aortic Endothelial Cells against Inflammatory Injury.
33050458	2	69	theme	chemical	404:411	arg1	composition					413:423	the chemical composition	400:423	the chemical composition of EEP-B	400:432	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	2	70	theme	aortic	366:371	arg1	MAECs					392:396	MAECs	392:396	MAECs	392:396	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	2	70	theme	aortic	366:371	arg1	cells					385:389	mouse aortic endothelial cells	360:389	mouse aortic endothelial cells (MAECs)	360:397	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	4	71	theme	3-isoprenyl	1032:1042	arg1	acid					1055:1058	3-isoprenyl p-coumaric acid	1032:1058	3-isoprenyl p-coumaric acid	1032:1058	The results showed that a total of 24 compounds belonging to cinnamic acids and flavonoids, including 3,5-diisopentenyl-4-hydroxycinnamic acid (artepillin C), kaempferide, 3-isoprenyl p-coumaric acid, pinocembrin and 4'-methoxy pinobanksin, were identified in EEP-B.
33050458	3	72	theme	enzyme-linked	741:753	arg1	assay					769:773	enzyme-linked immunosorbent assay	741:773	enzyme-linked immunosorbent assay (ELISA)	741:781	The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were analysed by western blotting.
33050458	3	72	theme	enzyme-linked	741:753	arg1	ELISA					776:780	ELISA	776:780	ELISA	776:780	The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were analysed by western blotting.
33050458	2	73	theme	protective	476:485	arg1	effect					487:492	the protective effect	472:492	the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs	472:564	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	3	74	theme	necrosis	672:679	arg1	TNF-α					691:695	TNF-α	691:695	TNF-α	691:695	The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were analysed by western blotting.
33050458	3	74	theme	necrosis	672:679	arg1	cytokines					655:663	inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6)	642:722	inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6)	642:722	The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were analysed by western blotting.
33050458	3	74	theme	necrosis	672:679	arg1	factor-α					681:688	tumour necrosis factor-α	665:688	tumour necrosis factor-α (TNF-α)	665:696	The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were analysed by western blotting.
33050458	5	75	theme	caffeic	1184:1190	arg1	ester					1213:1217	5-isoprenyl caffeic acid p-coumaric acid ester	1172:1217	5-isoprenyl caffeic acid p-coumaric acid ester	1172:1217	Among them, a new component, suggested to be 5-isoprenyl caffeic acid p-coumaric acid ester, was reported for the first time.
33050458	2	76	theme	mouse	360:364	arg1	MAECs					392:396	MAECs	392:396	MAECs	392:396	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	2	76	theme	mouse	360:364	arg1	cells					385:389	mouse aortic endothelial cells	360:389	mouse aortic endothelial cells (MAECs)	360:397	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	2	77	from	propolis	312:319	arg1	MAECs					392:396	MAECs	392:396	MAECs	392:396	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	2	77	from	propolis	312:319	arg1	cells					385:389	mouse aortic endothelial cells	360:389	mouse aortic endothelial cells (MAECs)	360:397	To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays.
33050458	3	78	theme	immunosorbent	755:767	arg1	assay					769:773	enzyme-linked immunosorbent assay	741:773	enzyme-linked immunosorbent assay (ELISA)	741:781	The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were analysed by western blotting.
33050458	3	78	theme	immunosorbent	755:767	arg1	ELISA					776:780	ELISA	776:780	ELISA	776:780	The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were analysed by western blotting.
34467572	2	0	theme	high	551:554	arg1	strength					556:563	high strength	551:563	high strength	551:563	Hereby, inspired by the impact-resistant dactyl club of the stomatopod, a mineralized biocomposite is rationally designed and produced in the complex shapes of dental implant crowns exhibiting high strength, stiffness, and fracture toughness.
34467572	1	1	theme	properties	298:307	arg1	combination					272:282	a combination	270:282	a combination of mechanical properties that are challenging to replicate synthetically	270:355	Nature provides unique insights into design strategies evolved by living organisms to construct robust materials with a combination of mechanical properties that are challenging to replicate synthetically.
34467572	0	2	theme	Engineered	97:106	arg1	Proteins					108:115	Genetically Engineered Proteins	85:115	Genetically Engineered Proteins with Controlled Biomineralization	85:149	Bioinspired Functionally Graded Composite Assembled Using Cellulose Nanocrystals and Genetically Engineered Proteins with Controlled Biomineralization.
34467572	5	3	theme	constitutive	1267:1278	arg1	protein					1280:1286	its constitutive protein	1263:1286	its constitutive protein building blocks	1263:1302	This work replicates multiscale biomanufacturing of a model biological material and also offers an innovative platform to synthesize multifunctional biocomposites whose properties can be finely regulated by colloidal self-assembly and engineering of its constitutive protein building blocks.
34467572	0	4	with	Proteins	108:115	arg1	Biomineralization					133:149	Controlled Biomineralization	122:149	Controlled Biomineralization	122:149	Bioinspired Functionally Graded Composite Assembled Using Cellulose Nanocrystals and Genetically Engineered Proteins with Controlled Biomineralization.
34467572	0	5	theme	Controlled	122:131	arg1	Biomineralization					133:149	Controlled Biomineralization	122:149	Controlled Biomineralization	122:149	Bioinspired Functionally Graded Composite Assembled Using Cellulose Nanocrystals and Genetically Engineered Proteins with Controlled Biomineralization.
34467572	5	6	theme	protein	1280:1286	arg1	engineering					1248:1258	engineering	1248:1258	engineering	1248:1258	This work replicates multiscale biomanufacturing of a model biological material and also offers an innovative platform to synthesize multifunctional biocomposites whose properties can be finely regulated by colloidal self-assembly and engineering of its constitutive protein building blocks.
34467572	5	6	theme	protein	1280:1286	arg1	self-assembly					1230:1242	colloidal self-assembly	1220:1242	colloidal self-assembly	1220:1242	This work replicates multiscale biomanufacturing of a model biological material and also offers an innovative platform to synthesize multifunctional biocomposites whose properties can be finely regulated by colloidal self-assembly and engineering of its constitutive protein building blocks.
34467572	4	7	theme	length	917:922	arg1	scales					924:929	multiple length scales	908:929	multiple length scales regulated by rational engineering and phase separation of the protein components	908:1010	Critically, the structural properties emerge from controlled self-assembly across multiple length scales regulated by rational engineering and phase separation of the protein components.
34467572	5	8	theme	model	1067:1071	arg1	material					1084:1091	a model biological material	1065:1091	a model biological material	1065:1091	This work replicates multiscale biomanufacturing of a model biological material and also offers an innovative platform to synthesize multifunctional biocomposites whose properties can be finely regulated by colloidal self-assembly and engineering of its constitutive protein building blocks.
34467572	5	9	theme	colloidal	1220:1228	arg1	self-assembly					1230:1242	colloidal self-assembly	1220:1242	colloidal self-assembly	1220:1242	This work replicates multiscale biomanufacturing of a model biological material and also offers an innovative platform to synthesize multifunctional biocomposites whose properties can be finely regulated by colloidal self-assembly and engineering of its constitutive protein building blocks.
34467572	4	10	theme	phase	969:973	arg1	separation					975:984	phase separation	969:984	phase separation	969:984	Critically, the structural properties emerge from controlled self-assembly across multiple length scales regulated by rational engineering and phase separation of the protein components.
34467572	5	11	theme	biological	1073:1082	arg1	material					1084:1091	a model biological material	1065:1091	a model biological material	1065:1091	This work replicates multiscale biomanufacturing of a model biological material and also offers an innovative platform to synthesize multifunctional biocomposites whose properties can be finely regulated by colloidal self-assembly and engineering of its constitutive protein building blocks.
34467572	4	12	theme	structural	842:851	arg1	properties					853:862	the structural properties	838:862	the structural properties	838:862	Critically, the structural properties emerge from controlled self-assembly across multiple length scales regulated by rational engineering and phase separation of the protein components.
34467572	3	13	theme	in	778:779	arg1	growth					786:791	in situ growth	778:791	in situ growth of reinforcing apatite crystals	778:823	This material consists of an expanded helicoidal organization of cellulose nanocrystals (CNCs) mixed with genetically engineered proteins that regulate both binding to CNCs and in situ growth of reinforcing apatite crystals.
34467572	0	14	theme	Bioinspired	0:10	arg1	Composite					32:40	Bioinspired Functionally Graded Composite	0:40	Bioinspired Functionally Graded Composite	0:40	Bioinspired Functionally Graded Composite Assembled Using Cellulose Nanocrystals and Genetically Engineered Proteins with Controlled Biomineralization.
34467572	1	15	theme	living	218:223	arg1	organisms					225:233	living organisms	218:233	living organisms	218:233	Nature provides unique insights into design strategies evolved by living organisms to construct robust materials with a combination of mechanical properties that are challenging to replicate synthetically.
34467572	4	16	theme	rational	944:951	arg1	engineering					953:963	rational engineering	944:963	rational engineering	944:963	Critically, the structural properties emerge from controlled self-assembly across multiple length scales regulated by rational engineering and phase separation of the protein components.
34467572	5	17	theme	multiscale	1034:1043	arg1	biomanufacturing					1045:1060	multiscale biomanufacturing	1034:1060	multiscale biomanufacturing of a model biological material	1034:1091	This work replicates multiscale biomanufacturing of a model biological material and also offers an innovative platform to synthesize multifunctional biocomposites whose properties can be finely regulated by colloidal self-assembly and engineering of its constitutive protein building blocks.
34467572	2	18	theme	mineralized	432:442	arg1	biocomposite					444:455	a mineralized biocomposite	430:455	a mineralized biocomposite	430:455	Hereby, inspired by the impact-resistant dactyl club of the stomatopod, a mineralized biocomposite is rationally designed and produced in the complex shapes of dental implant crowns exhibiting high strength, stiffness, and fracture toughness.
34467572	2	19	theme	complex	500:506	arg1	shapes					508:513	the complex shapes	496:513	the complex shapes of dental implant crowns exhibiting high strength, stiffness, and fracture toughness	496:598	Hereby, inspired by the impact-resistant dactyl club of the stomatopod, a mineralized biocomposite is rationally designed and produced in the complex shapes of dental implant crowns exhibiting high strength, stiffness, and fracture toughness.
34467572	3	20	theme	crystals	816:823	arg1	binding					758:764	binding	758:764	binding to CNCs	758:772	This material consists of an expanded helicoidal organization of cellulose nanocrystals (CNCs) mixed with genetically engineered proteins that regulate both binding to CNCs and in situ growth of reinforcing apatite crystals.
34467572	3	20	theme	crystals	816:823	arg1	growth					786:791	in situ growth	778:791	in situ growth of reinforcing apatite crystals	778:823	This material consists of an expanded helicoidal organization of cellulose nanocrystals (CNCs) mixed with genetically engineered proteins that regulate both binding to CNCs and in situ growth of reinforcing apatite crystals.
34467572	3	21	theme	expanded	630:637	arg1	organization					650:661	an expanded helicoidal organization	627:661	an expanded helicoidal organization of cellulose nanocrystals (CNCs) mixed with genetically engineered proteins that regulate both binding to CNCs and in situ growth of reinforcing apatite crystals	627:823	This material consists of an expanded helicoidal organization of cellulose nanocrystals (CNCs) mixed with genetically engineered proteins that regulate both binding to CNCs and in situ growth of reinforcing apatite crystals.
34467572	4	22	theme	controlled	876:885	arg1	self-assembly					887:899	controlled self-assembly	876:899	controlled self-assembly	876:899	Critically, the structural properties emerge from controlled self-assembly across multiple length scales regulated by rational engineering and phase separation of the protein components.
34467572	5	23	theme	multifunctional	1146:1160	arg1	biocomposites					1162:1174	multifunctional biocomposites	1146:1174	multifunctional biocomposites whose properties can be finely regulated by colloidal self-assembly and engineering of its constitutive protein building blocks	1146:1302	This work replicates multiscale biomanufacturing of a model biological material and also offers an innovative platform to synthesize multifunctional biocomposites whose properties can be finely regulated by colloidal self-assembly and engineering of its constitutive protein building blocks.
34467572	0	24	theme	Graded	25:30	arg1	Composite					32:40	Bioinspired Functionally Graded Composite	0:40	Bioinspired Functionally Graded Composite	0:40	Bioinspired Functionally Graded Composite Assembled Using Cellulose Nanocrystals and Genetically Engineered Proteins with Controlled Biomineralization.
34467572	3	25	theme	reinforcing	796:806	arg1	crystals					816:823	reinforcing apatite crystals	796:823	reinforcing apatite crystals	796:823	This material consists of an expanded helicoidal organization of cellulose nanocrystals (CNCs) mixed with genetically engineered proteins that regulate both binding to CNCs and in situ growth of reinforcing apatite crystals.
34467572	5	26	theme	material	1084:1091	arg1	biomanufacturing					1045:1060	multiscale biomanufacturing	1034:1060	multiscale biomanufacturing of a model biological material	1034:1091	This work replicates multiscale biomanufacturing of a model biological material and also offers an innovative platform to synthesize multifunctional biocomposites whose properties can be finely regulated by colloidal self-assembly and engineering of its constitutive protein building blocks.
34467572	1	27	theme	robust	248:253	arg1	materials					255:263	robust materials	248:263	robust materials	248:263	Nature provides unique insights into design strategies evolved by living organisms to construct robust materials with a combination of mechanical properties that are challenging to replicate synthetically.
34467572	2	28	theme	crowns	533:538	arg1	shapes					508:513	the complex shapes	496:513	the complex shapes of dental implant crowns exhibiting high strength, stiffness, and fracture toughness	496:598	Hereby, inspired by the impact-resistant dactyl club of the stomatopod, a mineralized biocomposite is rationally designed and produced in the complex shapes of dental implant crowns exhibiting high strength, stiffness, and fracture toughness.
34467572	3	29	theme	apatite	808:814	arg1	crystals					816:823	reinforcing apatite crystals	796:823	reinforcing apatite crystals	796:823	This material consists of an expanded helicoidal organization of cellulose nanocrystals (CNCs) mixed with genetically engineered proteins that regulate both binding to CNCs and in situ growth of reinforcing apatite crystals.
34467572	3	30	theme	engineered	719:728	arg1	proteins					730:737	genetically engineered proteins	707:737	genetically engineered proteins that regulate both binding to CNCs and in situ growth of reinforcing apatite crystals	707:823	This material consists of an expanded helicoidal organization of cellulose nanocrystals (CNCs) mixed with genetically engineered proteins that regulate both binding to CNCs and in situ growth of reinforcing apatite crystals.
34467572	2	31	theme	implant	525:531	arg1	crowns					533:538	dental implant crowns	518:538	dental implant crowns exhibiting high strength, stiffness, and fracture toughness	518:598	Hereby, inspired by the impact-resistant dactyl club of the stomatopod, a mineralized biocomposite is rationally designed and produced in the complex shapes of dental implant crowns exhibiting high strength, stiffness, and fracture toughness.
34467572	3	32	dep	in	778:779	arg1	situ					781:784	situ	781:784	situ	781:784	This material consists of an expanded helicoidal organization of cellulose nanocrystals (CNCs) mixed with genetically engineered proteins that regulate both binding to CNCs and in situ growth of reinforcing apatite crystals.
34467572	3	33	theme	cellulose	666:674	arg1	CNCs					690:693	CNCs	690:693	CNCs	690:693	This material consists of an expanded helicoidal organization of cellulose nanocrystals (CNCs) mixed with genetically engineered proteins that regulate both binding to CNCs and in situ growth of reinforcing apatite crystals.
34467572	3	33	theme	cellulose	666:674	arg1	nanocrystals					676:687	cellulose nanocrystals	666:687	cellulose nanocrystals (CNCs) mixed with genetically engineered proteins that regulate both binding to CNCs and in situ growth of reinforcing apatite crystals	666:823	This material consists of an expanded helicoidal organization of cellulose nanocrystals (CNCs) mixed with genetically engineered proteins that regulate both binding to CNCs and in situ growth of reinforcing apatite crystals.
34467572	2	34	theme	dental	518:523	arg1	crowns					533:538	dental implant crowns	518:538	dental implant crowns exhibiting high strength, stiffness, and fracture toughness	518:598	Hereby, inspired by the impact-resistant dactyl club of the stomatopod, a mineralized biocomposite is rationally designed and produced in the complex shapes of dental implant crowns exhibiting high strength, stiffness, and fracture toughness.
34467572	2	35	theme	fracture	581:588	arg1	toughness					590:598	fracture toughness	581:598	fracture toughness	581:598	Hereby, inspired by the impact-resistant dactyl club of the stomatopod, a mineralized biocomposite is rationally designed and produced in the complex shapes of dental implant crowns exhibiting high strength, stiffness, and fracture toughness.
34467572	3	36	theme	helicoidal	639:648	arg1	organization					650:661	an expanded helicoidal organization	627:661	an expanded helicoidal organization of cellulose nanocrystals (CNCs) mixed with genetically engineered proteins that regulate both binding to CNCs and in situ growth of reinforcing apatite crystals	627:823	This material consists of an expanded helicoidal organization of cellulose nanocrystals (CNCs) mixed with genetically engineered proteins that regulate both binding to CNCs and in situ growth of reinforcing apatite crystals.
34467572	0	37	theme	Cellulose	58:66	arg1	Nanocrystals					68:79	Cellulose Nanocrystals	58:79	Cellulose Nanocrystals	58:79	Bioinspired Functionally Graded Composite Assembled Using Cellulose Nanocrystals and Genetically Engineered Proteins with Controlled Biomineralization.
34467572	4	38	theme	multiple	908:915	arg1	scales					924:929	multiple length scales	908:929	multiple length scales regulated by rational engineering and phase separation of the protein components	908:1010	Critically, the structural properties emerge from controlled self-assembly across multiple length scales regulated by rational engineering and phase separation of the protein components.
34467572	2	39	theme	dactyl	399:404	arg1	club					406:409	the impact-resistant dactyl club	378:409	the impact-resistant dactyl club of the stomatopod	378:427	Hereby, inspired by the impact-resistant dactyl club of the stomatopod, a mineralized biocomposite is rationally designed and produced in the complex shapes of dental implant crowns exhibiting high strength, stiffness, and fracture toughness.
34467572	4	40	theme	components	1001:1010	arg1	engineering					953:963	rational engineering	944:963	rational engineering	944:963	Critically, the structural properties emerge from controlled self-assembly across multiple length scales regulated by rational engineering and phase separation of the protein components.
34467572	4	40	theme	components	1001:1010	arg1	separation					975:984	phase separation	969:984	phase separation	969:984	Critically, the structural properties emerge from controlled self-assembly across multiple length scales regulated by rational engineering and phase separation of the protein components.
34467572	1	41	theme	unique	168:173	arg1	insights					175:182	unique insights	168:182	unique insights into design strategies evolved by living organisms	168:233	Nature provides unique insights into design strategies evolved by living organisms to construct robust materials with a combination of mechanical properties that are challenging to replicate synthetically.
34467572	4	42	theme	protein	993:999	arg1	components					1001:1010	the protein components	989:1010	the protein components	989:1010	Critically, the structural properties emerge from controlled self-assembly across multiple length scales regulated by rational engineering and phase separation of the protein components.
34467572	0	43	with	Nanocrystals	68:79	arg1	Biomineralization					133:149	Controlled Biomineralization	122:149	Controlled Biomineralization	122:149	Bioinspired Functionally Graded Composite Assembled Using Cellulose Nanocrystals and Genetically Engineered Proteins with Controlled Biomineralization.
34467572	1	44	theme	design	189:194	arg1	strategies					196:205	design strategies	189:205	design strategies evolved by living organisms	189:233	Nature provides unique insights into design strategies evolved by living organisms to construct robust materials with a combination of mechanical properties that are challenging to replicate synthetically.
34467572	2	45	theme	impact-resistant	382:397	arg1	club					406:409	the impact-resistant dactyl club	378:409	the impact-resistant dactyl club of the stomatopod	378:427	Hereby, inspired by the impact-resistant dactyl club of the stomatopod, a mineralized biocomposite is rationally designed and produced in the complex shapes of dental implant crowns exhibiting high strength, stiffness, and fracture toughness.
34467572	3	46	theme	nanocrystals	676:687	arg1	organization					650:661	an expanded helicoidal organization	627:661	an expanded helicoidal organization of cellulose nanocrystals (CNCs) mixed with genetically engineered proteins that regulate both binding to CNCs and in situ growth of reinforcing apatite crystals	627:823	This material consists of an expanded helicoidal organization of cellulose nanocrystals (CNCs) mixed with genetically engineered proteins that regulate both binding to CNCs and in situ growth of reinforcing apatite crystals.
34467572	5	47	theme	innovative	1112:1121	arg1	platform					1123:1130	an innovative platform	1109:1130	an innovative platform to synthesize multifunctional biocomposites whose properties can be finely regulated by colloidal self-assembly and engineering of its constitutive protein building blocks	1109:1302	This work replicates multiscale biomanufacturing of a model biological material and also offers an innovative platform to synthesize multifunctional biocomposites whose properties can be finely regulated by colloidal self-assembly and engineering of its constitutive protein building blocks.
34467572	2	48	theme	stomatopod	418:427	arg1	club					406:409	the impact-resistant dactyl club	378:409	the impact-resistant dactyl club of the stomatopod	378:427	Hereby, inspired by the impact-resistant dactyl club of the stomatopod, a mineralized biocomposite is rationally designed and produced in the complex shapes of dental implant crowns exhibiting high strength, stiffness, and fracture toughness.
34467572	1	49	theme	mechanical	287:296	arg1	properties					298:307	mechanical properties	287:307	mechanical properties that are challenging to replicate synthetically	287:355	Nature provides unique insights into design strategies evolved by living organisms to construct robust materials with a combination of mechanical properties that are challenging to replicate synthetically.
32386244	0	0	theme	vortex-assisted	113:127	arg1	micro-solid-phase					149:165	vortex-assisted magnetic dispersive micro-solid-phase	113:165	vortex-assisted magnetic dispersive micro-solid-phase	113:165	Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan nanoparticles as an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction of celecoxib from human breast milk, plasma and urine samples.
32386244	1	1	theme	X-ray	444:448	arg1	diffraction					450:460	X-ray diffraction	444:460	X-ray diffraction	444:460	A polymer/layered silicate composite based on dimethyldioctadecylanimonium bentonite/chitosan magnetic nanoparticles was synthesized and characterized by field emission transmission electron microscopy, X-ray diffraction and Fourier transform infrared spectrometry.
32386244	0	2	theme	urine	226:230	arg1	samples					232:238	plasma and urine samples	215:238	plasma and urine samples	215:238	Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan nanoparticles as an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction of celecoxib from human breast milk, plasma and urine samples.
32386244	0	2	theme	urine	226:230	arg1	extraction					167:176	an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction	86:176	an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction of celecoxib from human breast milk	86:212	Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan nanoparticles as an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction of celecoxib from human breast milk, plasma and urine samples.
32386244	6	3	theme	%	1259:1259	arg1	range					1241:1245	the range	1237:1245	the range of 96.7-99.0%	1237:1259	Under the optimized conditions, the developed method exhibited wide linear ranges of 5-500 μg L-1 for plasma and urine and 10-500 μg L-1 for breast milk samples with satisfactory recoveries in the range of 96.7-99.0%.
32386244	6	4	theme	optimized	1054:1062	arg1	conditions					1064:1073	the optimized conditions	1050:1073	the optimized conditions	1050:1073	Under the optimized conditions, the developed method exhibited wide linear ranges of 5-500 μg L-1 for plasma and urine and 10-500 μg L-1 for breast milk samples with satisfactory recoveries in the range of 96.7-99.0%.
32386244	1	5	theme	magnetic	335:342	arg1	nanoparticles					344:356	dimethyldioctadecylanimonium bentonite/chitosan magnetic nanoparticles	287:356	dimethyldioctadecylanimonium bentonite/chitosan magnetic nanoparticles	287:356	A polymer/layered silicate composite based on dimethyldioctadecylanimonium bentonite/chitosan magnetic nanoparticles was synthesized and characterized by field emission transmission electron microscopy, X-ray diffraction and Fourier transform infrared spectrometry.
32386244	6	6	theme	developed	1080:1088	arg1	method					1090:1095	the developed method	1076:1095	the developed method	1076:1095	Under the optimized conditions, the developed method exhibited wide linear ranges of 5-500 μg L-1 for plasma and urine and 10-500 μg L-1 for breast milk samples with satisfactory recoveries in the range of 96.7-99.0%.
32386244	7	7	theme	quantification	1285:1298	arg1	Limit					1262:1266	Limit	1262:1266	Limit of detection and quantification of celecoxib	1262:1311	Limit of detection and quantification of celecoxib were in the ranges 0.3-3.2 and 0.99-10.56, respectively.
32386244	0	8	theme	dispersive	138:147	arg1	micro-solid-phase					149:165	vortex-assisted magnetic dispersive micro-solid-phase	113:165	vortex-assisted magnetic dispersive micro-solid-phase	113:165	Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan nanoparticles as an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction of celecoxib from human breast milk, plasma and urine samples.
32386244	4	9	theme	factorial	903:911	arg1	design					913:918	A fractional factorial design	890:918	A fractional factorial design	890:918	A fractional factorial design was utilized for screening the experimental parameters.
32386244	0	10	from	milk	209:212	arg1	samples					232:238	plasma and urine samples	215:238	plasma and urine samples	215:238	Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan nanoparticles as an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction of celecoxib from human breast milk, plasma and urine samples.
32386244	0	10	from	milk	209:212	arg1	extraction					167:176	an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction	86:176	an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction of celecoxib from human breast milk	86:212	Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan nanoparticles as an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction of celecoxib from human breast milk, plasma and urine samples.
32386244	0	11	theme	magnetic	129:136	arg1	micro-solid-phase					149:165	vortex-assisted magnetic dispersive micro-solid-phase	113:165	vortex-assisted magnetic dispersive micro-solid-phase	113:165	Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan nanoparticles as an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction of celecoxib from human breast milk, plasma and urine samples.
32386244	3	12	theme	magnetic	850:857	arg1	support					881:887	a magnetic chitosan nanoparticle support	848:887	a magnetic chitosan nanoparticle support	848:887	In this method, dimethyldioctadecylanimonium bentonite increases the accessibility of binding sites and adsorption capacity by high microporosity and large surface area, that has been realized for the first time in a magnetic chitosan nanoparticle support.
32386244	7	13	theme	detection	1271:1279	arg1	Limit					1262:1266	Limit	1262:1266	Limit of detection and quantification of celecoxib	1262:1311	Limit of detection and quantification of celecoxib were in the ranges 0.3-3.2 and 0.99-10.56, respectively.
32386244	4	14	theme	fractional	892:901	arg1	design					913:918	A fractional factorial design	890:918	A fractional factorial design	890:918	A fractional factorial design was utilized for screening the experimental parameters.
32386244	3	15	theme	chitosan	859:866	arg1	support					881:887	a magnetic chitosan nanoparticle support	848:887	a magnetic chitosan nanoparticle support	848:887	In this method, dimethyldioctadecylanimonium bentonite increases the accessibility of binding sites and adsorption capacity by high microporosity and large surface area, that has been realized for the first time in a magnetic chitosan nanoparticle support.
32386244	6	16	theme	satisfactory	1210:1221	arg1	recoveries					1223:1232	satisfactory recoveries	1210:1232	satisfactory recoveries in the range of 96.7-99.0%	1210:1259	Under the optimized conditions, the developed method exhibited wide linear ranges of 5-500 μg L-1 for plasma and urine and 10-500 μg L-1 for breast milk samples with satisfactory recoveries in the range of 96.7-99.0%.
32386244	8	17	theme	ranges	1414:1419	arg1	64.5-66.0					1421:1429	the ranges 64.5-66.0	1410:1429	the ranges 64.5-66.0	1410:1429	The enrichment factors were obtained in the ranges 64.5-66.0, while precisions were <3.7%.
32386244	3	18	from	time	840:843	arg1	support					881:887	a magnetic chitosan nanoparticle support	848:887	a magnetic chitosan nanoparticle support	848:887	In this method, dimethyldioctadecylanimonium bentonite increases the accessibility of binding sites and adsorption capacity by high microporosity and large surface area, that has been realized for the first time in a magnetic chitosan nanoparticle support.
32386244	3	19	theme	dimethyldioctadecylanimonium	649:676	arg1	bentonite					678:686	dimethyldioctadecylanimonium bentonite	649:686	dimethyldioctadecylanimonium bentonite	649:686	In this method, dimethyldioctadecylanimonium bentonite increases the accessibility of binding sites and adsorption capacity by high microporosity and large surface area, that has been realized for the first time in a magnetic chitosan nanoparticle support.
32386244	1	20	dep	Fourier	466:472	arg1	transform					474:482	transform	474:482	transform infrared spectrometry	474:504	A polymer/layered silicate composite based on dimethyldioctadecylanimonium bentonite/chitosan magnetic nanoparticles was synthesized and characterized by field emission transmission electron microscopy, X-ray diffraction and Fourier transform infrared spectrometry.
32386244	0	21	theme	bentonite	29:37	arg1	chitosan					60:67	Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan	0:67	Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan	0:67	Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan nanoparticles as an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction of celecoxib from human breast milk, plasma and urine samples.
32386244	5	22	theme	Box-Behnken	1024:1034	arg1	design					1036:1041	Box-Behnken design	1024:1041	Box-Behnken design	1024:1041	The effective parameters were then optimized by Box-Behnken design.
32386244	0	23	theme	Dimethyldioctadecylanimonium	0:27	arg1	chitosan					60:67	Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan	0:67	Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan	0:67	Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan nanoparticles as an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction of celecoxib from human breast milk, plasma and urine samples.
32386244	6	24	from	recoveries	1223:1232	arg1	range					1241:1245	the range	1237:1245	the range of 96.7-99.0%	1237:1259	Under the optimized conditions, the developed method exhibited wide linear ranges of 5-500 μg L-1 for plasma and urine and 10-500 μg L-1 for breast milk samples with satisfactory recoveries in the range of 96.7-99.0%.
32386244	3	25	theme	binding	719:725	arg1	sites					727:731	binding sites	719:731	binding sites	719:731	In this method, dimethyldioctadecylanimonium bentonite increases the accessibility of binding sites and adsorption capacity by high microporosity and large surface area, that has been realized for the first time in a magnetic chitosan nanoparticle support.
32386244	7	26	dep	ranges	1325:1330	arg1	0.99-10.56					1344:1353	0.99-10.56	1344:1353	0.99-10.56	1344:1353	Limit of detection and quantification of celecoxib were in the ranges 0.3-3.2 and 0.99-10.56, respectively.
32386244	7	26	dep	ranges	1325:1330	arg1	0.3-3.2					1332:1338	0.3-3.2	1332:1338	0.3-3.2	1332:1338	Limit of detection and quantification of celecoxib were in the ranges 0.3-3.2 and 0.99-10.56, respectively.
32386244	0	27	theme	magnetic	51:58	arg1	chitosan					60:67	Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan	0:67	Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan	0:67	Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan nanoparticles as an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction of celecoxib from human breast milk, plasma and urine samples.
32386244	6	28	theme	5-500 μg L-1	1129:1140	arg1	ranges					1119:1124	wide linear ranges	1107:1124	wide linear ranges of 5-500 μg L-1 for plasma and urine and 10-500 μg L-1 for breast milk samples with satisfactory recoveries in the range of 96.7-99.0%	1107:1259	Under the optimized conditions, the developed method exhibited wide linear ranges of 5-500 μg L-1 for plasma and urine and 10-500 μg L-1 for breast milk samples with satisfactory recoveries in the range of 96.7-99.0%.
32386244	0	29	theme	celecoxib	181:189	arg1	samples					232:238	plasma and urine samples	215:238	plasma and urine samples	215:238	Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan nanoparticles as an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction of celecoxib from human breast milk, plasma and urine samples.
32386244	0	29	theme	celecoxib	181:189	arg1	extraction					167:176	an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction	86:176	an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction of celecoxib from human breast milk	86:212	Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan nanoparticles as an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction of celecoxib from human breast milk, plasma and urine samples.
32386244	2	30	dep	samples	624:630	arg1	plasma					617:622	plasma	617:622	plasma	617:622	The prepared nanocomposite was used to isolate and preconcentrate celecoxib from human breast milk, urine and plasma samples.
32386244	2	30	dep	samples	624:630	arg1	urine					607:611	urine	607:611	urine	607:611	The prepared nanocomposite was used to isolate and preconcentrate celecoxib from human breast milk, urine and plasma samples.
32386244	2	30	dep	samples	624:630	arg1	samples					624:630	human breast milk, urine and plasma samples	588:630	samples	624:630	The prepared nanocomposite was used to isolate and preconcentrate celecoxib from human breast milk, urine and plasma samples.
32386244	5	31	theme	effective	980:988	arg1	parameters					990:999	The effective parameters	976:999	The effective parameters	976:999	The effective parameters were then optimized by Box-Behnken design.
32386244	0	32	theme	immobilized	39:49	arg1	chitosan					60:67	Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan	0:67	Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan	0:67	Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan nanoparticles as an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction of celecoxib from human breast milk, plasma and urine samples.
32386244	3	33	theme	nanoparticle	868:879	arg1	support					881:887	a magnetic chitosan nanoparticle support	848:887	a magnetic chitosan nanoparticle support	848:887	In this method, dimethyldioctadecylanimonium bentonite increases the accessibility of binding sites and adsorption capacity by high microporosity and large surface area, that has been realized for the first time in a magnetic chitosan nanoparticle support.
32386244	4	34	used	utilized	924:931	arg2	design					913:918	A fractional factorial design	890:918	A fractional factorial design	890:918	A fractional factorial design was utilized for screening the experimental parameters.
32386244	1	35	theme	polymer/layered	243:257	arg1	composite					268:276	A polymer/layered silicate composite	241:276	A polymer/layered silicate composite based on dimethyldioctadecylanimonium bentonite/chitosan magnetic nanoparticles	241:356	A polymer/layered silicate composite based on dimethyldioctadecylanimonium bentonite/chitosan magnetic nanoparticles was synthesized and characterized by field emission transmission electron microscopy, X-ray diffraction and Fourier transform infrared spectrometry.
32386244	8	36	theme	enrichment	1374:1383	arg1	factors					1385:1391	The enrichment factors	1370:1391	The enrichment factors	1370:1391	The enrichment factors were obtained in the ranges 64.5-66.0, while precisions were <3.7%.
32386244	1	37	theme	field	395:399	arg1	microscopy					432:441	field emission transmission electron microscopy	395:441	field emission transmission electron microscopy	395:441	A polymer/layered silicate composite based on dimethyldioctadecylanimonium bentonite/chitosan magnetic nanoparticles was synthesized and characterized by field emission transmission electron microscopy, X-ray diffraction and Fourier transform infrared spectrometry.
32386244	0	38	theme	human	196:200	arg1	milk					209:212	human breast milk	196:212	human breast milk	196:212	Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan nanoparticles as an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction of celecoxib from human breast milk, plasma and urine samples.
32386244	3	39	theme	adsorption	737:746	arg1	capacity					748:755	adsorption capacity	737:755	adsorption capacity	737:755	In this method, dimethyldioctadecylanimonium bentonite increases the accessibility of binding sites and adsorption capacity by high microporosity and large surface area, that has been realized for the first time in a magnetic chitosan nanoparticle support.
32386244	1	40	theme	silicate	259:266	arg1	composite					268:276	A polymer/layered silicate composite	241:276	A polymer/layered silicate composite based on dimethyldioctadecylanimonium bentonite/chitosan magnetic nanoparticles	241:356	A polymer/layered silicate composite based on dimethyldioctadecylanimonium bentonite/chitosan magnetic nanoparticles was synthesized and characterized by field emission transmission electron microscopy, X-ray diffraction and Fourier transform infrared spectrometry.
32386244	2	41	theme	milk	601:604	arg1	plasma					617:622	plasma	617:622	plasma	617:622	The prepared nanocomposite was used to isolate and preconcentrate celecoxib from human breast milk, urine and plasma samples.
32386244	2	41	theme	milk	601:604	arg1	urine					607:611	urine	607:611	urine	607:611	The prepared nanocomposite was used to isolate and preconcentrate celecoxib from human breast milk, urine and plasma samples.
32386244	2	41	theme	milk	601:604	arg1	samples					624:630	human breast milk, urine and plasma samples	588:630	samples	624:630	The prepared nanocomposite was used to isolate and preconcentrate celecoxib from human breast milk, urine and plasma samples.
32386244	1	42	theme	emission	401:408	arg1	microscopy					432:441	field emission transmission electron microscopy	395:441	field emission transmission electron microscopy	395:441	A polymer/layered silicate composite based on dimethyldioctadecylanimonium bentonite/chitosan magnetic nanoparticles was synthesized and characterized by field emission transmission electron microscopy, X-ray diffraction and Fourier transform infrared spectrometry.
32386244	2	43	from	samples	624:630	arg1	celecoxib					573:581	celecoxib	573:581	celecoxib from human breast milk, urine and plasma samples	573:630	The prepared nanocomposite was used to isolate and preconcentrate celecoxib from human breast milk, urine and plasma samples.
32386244	3	44	theme	capacity	748:755	arg1	accessibility					702:714	the accessibility	698:714	the accessibility of binding sites and adsorption capacity	698:755	In this method, dimethyldioctadecylanimonium bentonite increases the accessibility of binding sites and adsorption capacity by high microporosity and large surface area, that has been realized for the first time in a magnetic chitosan nanoparticle support.
32386244	2	45	theme	breast	594:599	arg1	plasma					617:622	plasma	617:622	plasma	617:622	The prepared nanocomposite was used to isolate and preconcentrate celecoxib from human breast milk, urine and plasma samples.
32386244	2	45	theme	breast	594:599	arg1	urine					607:611	urine	607:611	urine	607:611	The prepared nanocomposite was used to isolate and preconcentrate celecoxib from human breast milk, urine and plasma samples.
32386244	2	45	theme	breast	594:599	arg1	samples					624:630	human breast milk, urine and plasma samples	588:630	samples	624:630	The prepared nanocomposite was used to isolate and preconcentrate celecoxib from human breast milk, urine and plasma samples.
32386244	1	46	theme	transmission	410:421	arg1	microscopy					432:441	field emission transmission electron microscopy	395:441	field emission transmission electron microscopy	395:441	A polymer/layered silicate composite based on dimethyldioctadecylanimonium bentonite/chitosan magnetic nanoparticles was synthesized and characterized by field emission transmission electron microscopy, X-ray diffraction and Fourier transform infrared spectrometry.
32386244	3	47	theme	surface	789:795	arg1	area					797:800	large surface area	783:800	large surface area	783:800	In this method, dimethyldioctadecylanimonium bentonite increases the accessibility of binding sites and adsorption capacity by high microporosity and large surface area, that has been realized for the first time in a magnetic chitosan nanoparticle support.
32386244	3	48	theme	sites	727:731	arg1	accessibility					702:714	the accessibility	698:714	the accessibility of binding sites and adsorption capacity	698:755	In this method, dimethyldioctadecylanimonium bentonite increases the accessibility of binding sites and adsorption capacity by high microporosity and large surface area, that has been realized for the first time in a magnetic chitosan nanoparticle support.
32386244	2	49	used	used	538:541	arg2	nanocomposite					520:532	The prepared nanocomposite	507:532	The prepared nanocomposite	507:532	The prepared nanocomposite was used to isolate and preconcentrate celecoxib from human breast milk, urine and plasma samples.
32386244	6	50	theme	linear	1112:1117	arg1	ranges					1119:1124	wide linear ranges	1107:1124	wide linear ranges of 5-500 μg L-1 for plasma and urine and 10-500 μg L-1 for breast milk samples with satisfactory recoveries in the range of 96.7-99.0%	1107:1259	Under the optimized conditions, the developed method exhibited wide linear ranges of 5-500 μg L-1 for plasma and urine and 10-500 μg L-1 for breast milk samples with satisfactory recoveries in the range of 96.7-99.0%.
32386244	2	51	theme	human	588:592	arg1	plasma					617:622	plasma	617:622	plasma	617:622	The prepared nanocomposite was used to isolate and preconcentrate celecoxib from human breast milk, urine and plasma samples.
32386244	2	51	theme	human	588:592	arg1	urine					607:611	urine	607:611	urine	607:611	The prepared nanocomposite was used to isolate and preconcentrate celecoxib from human breast milk, urine and plasma samples.
32386244	2	51	theme	human	588:592	arg1	samples					624:630	human breast milk, urine and plasma samples	588:630	samples	624:630	The prepared nanocomposite was used to isolate and preconcentrate celecoxib from human breast milk, urine and plasma samples.
32386244	1	52	theme	electron	423:430	arg1	microscopy					432:441	field emission transmission electron microscopy	395:441	field emission transmission electron microscopy	395:441	A polymer/layered silicate composite based on dimethyldioctadecylanimonium bentonite/chitosan magnetic nanoparticles was synthesized and characterized by field emission transmission electron microscopy, X-ray diffraction and Fourier transform infrared spectrometry.
32386244	0	53	theme	breast	202:207	arg1	milk					209:212	human breast milk	196:212	human breast milk	196:212	Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan nanoparticles as an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction of celecoxib from human breast milk, plasma and urine samples.
32386244	3	54	theme	large	783:787	arg1	area					797:800	large surface area	783:800	large surface area	783:800	In this method, dimethyldioctadecylanimonium bentonite increases the accessibility of binding sites and adsorption capacity by high microporosity and large surface area, that has been realized for the first time in a magnetic chitosan nanoparticle support.
32386244	1	55	dep	transform	474:482	arg1	infrared					484:491	infrared	484:491	transform infrared spectrometry	474:504	A polymer/layered silicate composite based on dimethyldioctadecylanimonium bentonite/chitosan magnetic nanoparticles was synthesized and characterized by field emission transmission electron microscopy, X-ray diffraction and Fourier transform infrared spectrometry.
32386244	0	56	theme	plasma	215:220	arg1	samples					232:238	plasma and urine samples	215:238	plasma and urine samples	215:238	Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan nanoparticles as an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction of celecoxib from human breast milk, plasma and urine samples.
32386244	0	56	theme	plasma	215:220	arg1	extraction					167:176	an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction	86:176	an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction of celecoxib from human breast milk	86:212	Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan nanoparticles as an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction of celecoxib from human breast milk, plasma and urine samples.
32386244	6	57	with	samples	1197:1203	arg1	recoveries					1223:1232	satisfactory recoveries	1210:1232	satisfactory recoveries in the range of 96.7-99.0%	1210:1259	Under the optimized conditions, the developed method exhibited wide linear ranges of 5-500 μg L-1 for plasma and urine and 10-500 μg L-1 for breast milk samples with satisfactory recoveries in the range of 96.7-99.0%.
32386244	7	58	theme	celecoxib	1303:1311	arg1	detection					1271:1279	detection	1271:1279	detection	1271:1279	Limit of detection and quantification of celecoxib were in the ranges 0.3-3.2 and 0.99-10.56, respectively.
32386244	7	58	theme	celecoxib	1303:1311	arg1	quantification					1285:1298	quantification	1285:1298	quantification	1285:1298	Limit of detection and quantification of celecoxib were in the ranges 0.3-3.2 and 0.99-10.56, respectively.
32386244	4	59	theme	experimental	951:962	arg1	parameters					964:973	the experimental parameters	947:973	the experimental parameters	947:973	A fractional factorial design was utilized for screening the experimental parameters.
32386244	6	60	theme	milk	1192:1195	arg1	samples					1197:1203	breast milk samples	1185:1203	breast milk samples with satisfactory recoveries in the range of 96.7-99.0%	1185:1259	Under the optimized conditions, the developed method exhibited wide linear ranges of 5-500 μg L-1 for plasma and urine and 10-500 μg L-1 for breast milk samples with satisfactory recoveries in the range of 96.7-99.0%.
32386244	6	61	theme	breast	1185:1190	arg1	samples					1197:1203	breast milk samples	1185:1203	breast milk samples with satisfactory recoveries in the range of 96.7-99.0%	1185:1259	Under the optimized conditions, the developed method exhibited wide linear ranges of 5-500 μg L-1 for plasma and urine and 10-500 μg L-1 for breast milk samples with satisfactory recoveries in the range of 96.7-99.0%.
32386244	0	62	theme	adsorbent	99:107	arg1	samples					232:238	plasma and urine samples	215:238	plasma and urine samples	215:238	Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan nanoparticles as an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction of celecoxib from human breast milk, plasma and urine samples.
32386244	0	62	theme	adsorbent	99:107	arg1	extraction					167:176	an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction	86:176	an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction of celecoxib from human breast milk	86:212	Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan nanoparticles as an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction of celecoxib from human breast milk, plasma and urine samples.
32386244	2	63	theme	prepared	511:518	arg1	nanocomposite					520:532	The prepared nanocomposite	507:532	The prepared nanocomposite	507:532	The prepared nanocomposite was used to isolate and preconcentrate celecoxib from human breast milk, urine and plasma samples.
32386244	1	64	theme	dimethyldioctadecylanimonium	287:314	arg1	nanoparticles					344:356	dimethyldioctadecylanimonium bentonite/chitosan magnetic nanoparticles	287:356	dimethyldioctadecylanimonium bentonite/chitosan magnetic nanoparticles	287:356	A polymer/layered silicate composite based on dimethyldioctadecylanimonium bentonite/chitosan magnetic nanoparticles was synthesized and characterized by field emission transmission electron microscopy, X-ray diffraction and Fourier transform infrared spectrometry.
32386244	0	65	theme	efficient	89:97	arg1	samples					232:238	plasma and urine samples	215:238	plasma and urine samples	215:238	Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan nanoparticles as an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction of celecoxib from human breast milk, plasma and urine samples.
32386244	0	65	theme	efficient	89:97	arg1	extraction					167:176	an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction	86:176	an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction of celecoxib from human breast milk	86:212	Dimethyldioctadecylanimonium bentonite immobilized magnetic chitosan nanoparticles as an efficient adsorbent for vortex-assisted magnetic dispersive micro-solid-phase extraction of celecoxib from human breast milk, plasma and urine samples.
32386244	3	66	theme	first	834:838	arg1	time					840:843	the first time	830:843	the first time in a magnetic chitosan nanoparticle support	830:887	In this method, dimethyldioctadecylanimonium bentonite increases the accessibility of binding sites and adsorption capacity by high microporosity and large surface area, that has been realized for the first time in a magnetic chitosan nanoparticle support.
32386244	3	67	theme	high	760:763	arg1	microporosity					765:777	high microporosity	760:777	high microporosity	760:777	In this method, dimethyldioctadecylanimonium bentonite increases the accessibility of binding sites and adsorption capacity by high microporosity and large surface area, that has been realized for the first time in a magnetic chitosan nanoparticle support.
32386244	6	68	theme	wide	1107:1110	arg1	ranges					1119:1124	wide linear ranges	1107:1124	wide linear ranges of 5-500 μg L-1 for plasma and urine and 10-500 μg L-1 for breast milk samples with satisfactory recoveries in the range of 96.7-99.0%	1107:1259	Under the optimized conditions, the developed method exhibited wide linear ranges of 5-500 μg L-1 for plasma and urine and 10-500 μg L-1 for breast milk samples with satisfactory recoveries in the range of 96.7-99.0%.
32386244	1	69	theme	bentonite/chitosan	316:333	arg1	nanoparticles					344:356	dimethyldioctadecylanimonium bentonite/chitosan magnetic nanoparticles	287:356	dimethyldioctadecylanimonium bentonite/chitosan magnetic nanoparticles	287:356	A polymer/layered silicate composite based on dimethyldioctadecylanimonium bentonite/chitosan magnetic nanoparticles was synthesized and characterized by field emission transmission electron microscopy, X-ray diffraction and Fourier transform infrared spectrometry.
33587639	3	0	theme	butyrate	734:741	arg1	production					743:752	Elevated butyrate production	725:752	Elevated butyrate production	725:752	Elevated butyrate production further increased the acetylation of histone proteins H3 and H4 and thus enhanced the transcription of the major colonic mucin gene Muc2 and the secretion of mucin elements, which represents a new molecular mechanism of KGM oligosaccharide consumption.
33587639	2	1	theme	colonic	707:713	arg1	butyrate					715:722	colonic butyrate	707:722	colonic butyrate	707:722	The results are the first to reveal that KGMOS regulated intestinal microflora composition to facilitate the production of colonic butyrate.
33587639	3	2	theme	Muc2	886:889	arg1	secretion					899:907	the secretion	895:907	the secretion of mucin elements, which represents a new molecular mechanism of KGM oligosaccharide consumption	895:1004	Elevated butyrate production further increased the acetylation of histone proteins H3 and H4 and thus enhanced the transcription of the major colonic mucin gene Muc2 and the secretion of mucin elements, which represents a new molecular mechanism of KGM oligosaccharide consumption.
33587639	3	2	theme	Muc2	886:889	arg1	transcription					840:852	the transcription	836:852	the transcription of the major colonic mucin gene Muc2	836:889	Elevated butyrate production further increased the acetylation of histone proteins H3 and H4 and thus enhanced the transcription of the major colonic mucin gene Muc2 and the secretion of mucin elements, which represents a new molecular mechanism of KGM oligosaccharide consumption.
33587639	1	3	theme	high	269:272	arg1	weight					284:289	a relatively high molecular weight	256:289	a relatively high molecular weight	256:289	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice.
33587639	0	4	theme	Barrier	91:97	arg1	Function					99:106	Colonic Mucosal Barrier Function	75:106	Colonic Mucosal Barrier Function	75:106	A Purified Glucomannan Oligosaccharide from Amorphophallus konjac Improves Colonic Mucosal Barrier Function via Enhancing Butyrate Production and Histone Protein H3 and H4 Acetylation.
33587639	2	5	theme	regulated	631:639	arg1	microflora					652:661	KGMOS regulated intestinal microflora	625:661	KGMOS regulated intestinal microflora	625:661	The results are the first to reveal that KGMOS regulated intestinal microflora composition to facilitate the production of colonic butyrate.
33587639	3	6	theme	oligosaccharide	978:992	arg1	consumption					994:1004	KGM oligosaccharide consumption	974:1004	KGM oligosaccharide consumption	974:1004	Elevated butyrate production further increased the acetylation of histone proteins H3 and H4 and thus enhanced the transcription of the major colonic mucin gene Muc2 and the secretion of mucin elements, which represents a new molecular mechanism of KGM oligosaccharide consumption.
33587639	3	7	theme	colonic	867:873	arg1	Muc2					886:889	the major colonic mucin gene Muc2	857:889	the major colonic mucin gene Muc2	857:889	Elevated butyrate production further increased the acetylation of histone proteins H3 and H4 and thus enhanced the transcription of the major colonic mucin gene Muc2 and the secretion of mucin elements, which represents a new molecular mechanism of KGM oligosaccharide consumption.
33587639	1	8	theme	molecular	274:282	arg1	weight					284:289	a relatively high molecular weight	256:289	a relatively high molecular weight	256:289	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice.
33587639	3	9	theme	consumption	994:1004	arg1	mechanism					961:969	a new molecular mechanism	945:969	a new molecular mechanism of KGM oligosaccharide consumption	945:1004	Elevated butyrate production further increased the acetylation of histone proteins H3 and H4 and thus enhanced the transcription of the major colonic mucin gene Muc2 and the secretion of mucin elements, which represents a new molecular mechanism of KGM oligosaccharide consumption.
33587639	1	10	theme	native	434:439	arg1	KGM					461:463	KGM	461:463	KGM	461:463	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice.
33587639	1	10	theme	native	434:439	arg1	glucomannan					448:458	native konjac glucomannan	434:458	native konjac glucomannan (KGM)	434:464	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice.
33587639	3	11	theme	mucin	875:879	arg1	Muc2					886:889	the major colonic mucin gene Muc2	857:889	the major colonic mucin gene Muc2	857:889	Elevated butyrate production further increased the acetylation of histone proteins H3 and H4 and thus enhanced the transcription of the major colonic mucin gene Muc2 and the secretion of mucin elements, which represents a new molecular mechanism of KGM oligosaccharide consumption.
33587639	1	12	theme	konjac	441:446	arg1	KGM					461:463	KGM	461:463	KGM	461:463	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice.
33587639	1	12	theme	konjac	441:446	arg1	glucomannan					448:458	native konjac glucomannan	434:458	native konjac glucomannan (KGM)	434:464	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice.
33587639	1	13	theme	KGMOS	522:526	arg1	effects					486:492	the beneficial effects	471:492	the beneficial effects	471:492	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice.
33587639	1	13	theme	KGMOS	522:526	arg1	mechanisms					508:517	molecular mechanisms	498:517	molecular mechanisms	498:517	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice.
33587639	0	14	from	konjac	59:64	arg1	Oligosaccharide					23:37	A Purified Glucomannan Oligosaccharide	0:37	A Purified Glucomannan Oligosaccharide from Amorphophallus konjac	0:64	A Purified Glucomannan Oligosaccharide from Amorphophallus konjac Improves Colonic Mucosal Barrier Function via Enhancing Butyrate Production and Histone Protein H3 and H4 Acetylation.
33587639	1	15	from	mechanisms	508:517	arg1	functions					539:547	colonic functions	531:547	colonic functions	531:547	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice.
33587639	0	16	theme	Butyrate	122:129	arg1	Production					131:140	Butyrate Production	122:140	Butyrate Production	122:140	A Purified Glucomannan Oligosaccharide from Amorphophallus konjac Improves Colonic Mucosal Barrier Function via Enhancing Butyrate Production and Histone Protein H3 and H4 Acetylation.
33587639	4	17	theme	barrier	1187:1193	arg1	function					1195:1202	the barrier function	1183:1202	the barrier function of the colonic mucosa	1183:1224	The findings indicate that KGM oligosaccharides with specific molecular sizes have highly desirable functional properties and potentially could improve gut health by promoting the barrier function of the colonic mucosa.
33587639	1	18	theme	colonic	531:537	arg1	functions					539:547	colonic functions	531:547	colonic functions	531:547	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice.
33587639	0	19	theme	Purified	2:9	arg1	Oligosaccharide					23:37	A Purified Glucomannan Oligosaccharide	0:37	A Purified Glucomannan Oligosaccharide from Amorphophallus konjac	0:64	A Purified Glucomannan Oligosaccharide from Amorphophallus konjac Improves Colonic Mucosal Barrier Function via Enhancing Butyrate Production and Histone Protein H3 and H4 Acetylation.
33587639	4	20	contain	have	1085:1088	arg2	properties					1118:1127	highly desirable functional properties	1090:1127	highly desirable functional properties	1090:1127	The findings indicate that KGM oligosaccharides with specific molecular sizes have highly desirable functional properties and potentially could improve gut health by promoting the barrier function of the colonic mucosa.
33587639	4	20	contain	have	1085:1088	arg1	oligosaccharides					1038:1053	KGM oligosaccharides	1034:1053	KGM oligosaccharides with specific molecular sizes	1034:1083	The findings indicate that KGM oligosaccharides with specific molecular sizes have highly desirable functional properties and potentially could improve gut health by promoting the barrier function of the colonic mucosa.
33587639	3	21	theme	Elevated	725:732	arg1	production					743:752	Elevated butyrate production	725:752	Elevated butyrate production	725:752	Elevated butyrate production further increased the acetylation of histone proteins H3 and H4 and thus enhanced the transcription of the major colonic mucin gene Muc2 and the secretion of mucin elements, which represents a new molecular mechanism of KGM oligosaccharide consumption.
33587639	1	22	theme	defined	200:206	arg1	oligosaccharide					227:241	A structurally defined konjac glucomannan oligosaccharide	185:241	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11)	185:414	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice.
33587639	1	22	theme	defined	200:206	arg1	KGMOS					244:248	KGMOS	244:248	KGMOS	244:248	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice.
33587639	1	23	theme	narrow	295:300	arg1	distribution					319:330	narrow molecular weight distribution	295:330	narrow molecular weight distribution	295:330	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice.
33587639	1	24	theme	konjac	208:213	arg1	oligosaccharide					227:241	A structurally defined konjac glucomannan oligosaccharide	185:241	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11)	185:414	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice.
33587639	1	24	theme	konjac	208:213	arg1	KGMOS					244:248	KGMOS	244:248	KGMOS	244:248	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice.
33587639	4	25	theme	gut	1159:1161	arg1	health					1163:1168	gut health	1159:1168	gut health	1159:1168	The findings indicate that KGM oligosaccharides with specific molecular sizes have highly desirable functional properties and potentially could improve gut health by promoting the barrier function of the colonic mucosa.
33587639	1	26	theme	molecular	302:310	arg1	distribution					319:330	narrow molecular weight distribution	295:330	narrow molecular weight distribution	295:330	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice.
33587639	0	27	theme	Histone	146:152	arg1	H3					162:163	Histone Protein H3 and H4 Acetylation	146:182	H3	162:163	A Purified Glucomannan Oligosaccharide from Amorphophallus konjac Improves Colonic Mucosal Barrier Function via Enhancing Butyrate Production and Histone Protein H3 and H4 Acetylation.
33587639	2	28	theme	intestinal	641:650	arg1	microflora					652:661	KGMOS regulated intestinal microflora	625:661	KGMOS regulated intestinal microflora	625:661	The results are the first to reveal that KGMOS regulated intestinal microflora composition to facilitate the production of colonic butyrate.
33587639	4	29	with	oligosaccharides	1038:1053	arg1	sizes					1079:1083	specific molecular sizes	1060:1083	specific molecular sizes	1060:1083	The findings indicate that KGM oligosaccharides with specific molecular sizes have highly desirable functional properties and potentially could improve gut health by promoting the barrier function of the colonic mucosa.
33587639	1	30	theme	glucomannan	215:225	arg1	oligosaccharide					227:241	A structurally defined konjac glucomannan oligosaccharide	185:241	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11)	185:414	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice.
33587639	1	30	theme	glucomannan	215:225	arg1	KGMOS					244:248	KGMOS	244:248	KGMOS	244:248	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice.
33587639	0	31	theme	Glucomannan	11:21	arg1	Oligosaccharide					23:37	A Purified Glucomannan Oligosaccharide	0:37	A Purified Glucomannan Oligosaccharide from Amorphophallus konjac	0:64	A Purified Glucomannan Oligosaccharide from Amorphophallus konjac Improves Colonic Mucosal Barrier Function via Enhancing Butyrate Production and Histone Protein H3 and H4 Acetylation.
33587639	1	32	theme	weight	312:317	arg1	distribution					319:330	narrow molecular weight distribution	295:330	narrow molecular weight distribution	295:330	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice.
33587639	4	33	theme	mucosa	1219:1224	arg1	function					1195:1202	the barrier function	1183:1202	the barrier function of the colonic mucosa	1183:1224	The findings indicate that KGM oligosaccharides with specific molecular sizes have highly desirable functional properties and potentially could improve gut health by promoting the barrier function of the colonic mucosa.
33587639	1	34	theme	polymerization	390:403	arg1	degree					380:385	degree	380:385	degree of polymerization (dp) 8-11	380:413	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice.
33587639	3	35	theme	elements	918:925	arg1	secretion					899:907	the secretion	895:907	the secretion of mucin elements, which represents a new molecular mechanism of KGM oligosaccharide consumption	895:1004	Elevated butyrate production further increased the acetylation of histone proteins H3 and H4 and thus enhanced the transcription of the major colonic mucin gene Muc2 and the secretion of mucin elements, which represents a new molecular mechanism of KGM oligosaccharide consumption.
33587639	3	35	theme	elements	918:925	arg1	transcription					840:852	the transcription	836:852	the transcription of the major colonic mucin gene Muc2	836:889	Elevated butyrate production further increased the acetylation of histone proteins H3 and H4 and thus enhanced the transcription of the major colonic mucin gene Muc2 and the secretion of mucin elements, which represents a new molecular mechanism of KGM oligosaccharide consumption.
33587639	3	36	theme	mucin	912:916	arg1	elements					918:925	mucin elements	912:925	mucin elements	912:925	Elevated butyrate production further increased the acetylation of histone proteins H3 and H4 and thus enhanced the transcription of the major colonic mucin gene Muc2 and the secretion of mucin elements, which represents a new molecular mechanism of KGM oligosaccharide consumption.
33587639	1	37	from	effects	486:492	arg1	functions					539:547	colonic functions	531:547	colonic functions	531:547	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice.
33587639	0	38	theme	Amorphophallus	44:57	arg1	konjac					59:64	Amorphophallus konjac	44:64	Amorphophallus konjac	44:64	A Purified Glucomannan Oligosaccharide from Amorphophallus konjac Improves Colonic Mucosal Barrier Function via Enhancing Butyrate Production and Histone Protein H3 and H4 Acetylation.
33587639	1	39	theme	C57BL/6	570:576	arg1	mice					578:581	C57BL/6 mice	570:581	C57BL/6 mice	570:581	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice.
33587639	0	40	theme	Protein	154:160	arg1	H3					162:163	Histone Protein H3 and H4 Acetylation	146:182	H3	162:163	A Purified Glucomannan Oligosaccharide from Amorphophallus konjac Improves Colonic Mucosal Barrier Function via Enhancing Butyrate Production and Histone Protein H3 and H4 Acetylation.
33587639	4	41	theme	specific	1060:1067	arg1	sizes					1079:1083	specific molecular sizes	1060:1083	specific molecular sizes	1060:1083	The findings indicate that KGM oligosaccharides with specific molecular sizes have highly desirable functional properties and potentially could improve gut health by promoting the barrier function of the colonic mucosa.
33587639	3	42	theme	new	947:949	arg1	mechanism					961:969	a new molecular mechanism	945:969	a new molecular mechanism of KGM oligosaccharide consumption	945:1004	Elevated butyrate production further increased the acetylation of histone proteins H3 and H4 and thus enhanced the transcription of the major colonic mucin gene Muc2 and the secretion of mucin elements, which represents a new molecular mechanism of KGM oligosaccharide consumption.
33587639	1	43	theme	molecular	333:341	arg1	weight					343:348	molecular weight	333:348	molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11	333:413	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice.
33587639	0	44	theme	H4	169:170	arg1	Acetylation					172:182	Histone Protein H3 and H4 Acetylation	146:182	Acetylation	172:182	A Purified Glucomannan Oligosaccharide from Amorphophallus konjac Improves Colonic Mucosal Barrier Function via Enhancing Butyrate Production and Histone Protein H3 and H4 Acetylation.
33587639	1	45	dep	4000	371:374	arg1	to					368:369	to	368:369	to	368:369	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice.
33587639	3	46	theme	molecular	951:959	arg1	mechanism					961:969	a new molecular mechanism	945:969	a new molecular mechanism of KGM oligosaccharide consumption	945:1004	Elevated butyrate production further increased the acetylation of histone proteins H3 and H4 and thus enhanced the transcription of the major colonic mucin gene Muc2 and the secretion of mucin elements, which represents a new molecular mechanism of KGM oligosaccharide consumption.
33587639	1	47	with	oligosaccharide	227:241	arg1	distribution					319:330	narrow molecular weight distribution	295:330	narrow molecular weight distribution	295:330	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice.
33587639	1	47	with	oligosaccharide	227:241	arg1	weight					284:289	a relatively high molecular weight	256:289	a relatively high molecular weight	256:289	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice.
33587639	1	48	dep	weight	284:289	arg1	weight					343:348	molecular weight	333:348	molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11	333:413	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice.
33587639	1	49	theme	beneficial	475:484	arg1	effects					486:492	the beneficial effects	471:492	the beneficial effects	471:492	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice.
33587639	2	50	theme	KGMOS	625:629	arg1	microflora					652:661	KGMOS regulated intestinal microflora	625:661	KGMOS regulated intestinal microflora	625:661	The results are the first to reveal that KGMOS regulated intestinal microflora composition to facilitate the production of colonic butyrate.
33587639	3	51	theme	major	861:865	arg1	Muc2					886:889	the major colonic mucin gene Muc2	857:889	the major colonic mucin gene Muc2	857:889	Elevated butyrate production further increased the acetylation of histone proteins H3 and H4 and thus enhanced the transcription of the major colonic mucin gene Muc2 and the secretion of mucin elements, which represents a new molecular mechanism of KGM oligosaccharide consumption.
33587639	4	52	theme	colonic	1211:1217	arg1	mucosa					1219:1224	the colonic mucosa	1207:1224	the colonic mucosa	1207:1224	The findings indicate that KGM oligosaccharides with specific molecular sizes have highly desirable functional properties and potentially could improve gut health by promoting the barrier function of the colonic mucosa.
33587639	3	53	theme	KGM	974:976	arg1	consumption					994:1004	KGM oligosaccharide consumption	974:1004	KGM oligosaccharide consumption	974:1004	Elevated butyrate production further increased the acetylation of histone proteins H3 and H4 and thus enhanced the transcription of the major colonic mucin gene Muc2 and the secretion of mucin elements, which represents a new molecular mechanism of KGM oligosaccharide consumption.
33587639	4	54	theme	functional	1107:1116	arg1	properties					1118:1127	highly desirable functional properties	1090:1127	highly desirable functional properties	1090:1127	The findings indicate that KGM oligosaccharides with specific molecular sizes have highly desirable functional properties and potentially could improve gut health by promoting the barrier function of the colonic mucosa.
33587639	0	55	theme	Mucosal	83:89	arg1	Function					99:106	Colonic Mucosal Barrier Function	75:106	Colonic Mucosal Barrier Function	75:106	A Purified Glucomannan Oligosaccharide from Amorphophallus konjac Improves Colonic Mucosal Barrier Function via Enhancing Butyrate Production and Histone Protein H3 and H4 Acetylation.
33587639	4	56	theme	molecular	1069:1077	arg1	sizes					1079:1083	specific molecular sizes	1060:1083	specific molecular sizes	1060:1083	The findings indicate that KGM oligosaccharides with specific molecular sizes have highly desirable functional properties and potentially could improve gut health by promoting the barrier function of the colonic mucosa.
33587639	3	57	dep	proteins	799:806	arg1	proteins					799:806	histone proteins H3 and H4	791:816	histone proteins H3 and H4	791:816	Elevated butyrate production further increased the acetylation of histone proteins H3 and H4 and thus enhanced the transcription of the major colonic mucin gene Muc2 and the secretion of mucin elements, which represents a new molecular mechanism of KGM oligosaccharide consumption.
33587639	3	57	dep	proteins	799:806	arg1	H4					815:816	H4	815:816	H4	815:816	Elevated butyrate production further increased the acetylation of histone proteins H3 and H4 and thus enhanced the transcription of the major colonic mucin gene Muc2 and the secretion of mucin elements, which represents a new molecular mechanism of KGM oligosaccharide consumption.
33587639	3	57	dep	proteins	799:806	arg1	H3					808:809	H3	808:809	H3	808:809	Elevated butyrate production further increased the acetylation of histone proteins H3 and H4 and thus enhanced the transcription of the major colonic mucin gene Muc2 and the secretion of mucin elements, which represents a new molecular mechanism of KGM oligosaccharide consumption.
33587639	1	58	theme	molecular	498:506	arg1	mechanisms					508:517	molecular mechanisms	498:517	molecular mechanisms	498:517	A structurally defined konjac glucomannan oligosaccharide (KGMOS) with a relatively high molecular weight and narrow molecular weight distribution (molecular weight ranging from 3000 to 4000 Da, degree of polymerization (dp) 8-11) was prepared from native konjac glucomannan (KGM), and the beneficial effects and molecular mechanisms of KGMOS on colonic functions were investigated in C57BL/6 mice.
33587639	4	59	theme	desirable	1097:1105	arg1	properties					1118:1127	highly desirable functional properties	1090:1127	highly desirable functional properties	1090:1127	The findings indicate that KGM oligosaccharides with specific molecular sizes have highly desirable functional properties and potentially could improve gut health by promoting the barrier function of the colonic mucosa.
33587639	3	60	theme	proteins	799:806	arg1	acetylation					776:786	the acetylation	772:786	the acetylation of histone proteins H3 and H4	772:816	Elevated butyrate production further increased the acetylation of histone proteins H3 and H4 and thus enhanced the transcription of the major colonic mucin gene Muc2 and the secretion of mucin elements, which represents a new molecular mechanism of KGM oligosaccharide consumption.
33587639	0	61	theme	Colonic	75:81	arg1	Function					99:106	Colonic Mucosal Barrier Function	75:106	Colonic Mucosal Barrier Function	75:106	A Purified Glucomannan Oligosaccharide from Amorphophallus konjac Improves Colonic Mucosal Barrier Function via Enhancing Butyrate Production and Histone Protein H3 and H4 Acetylation.
33587639	4	62	theme	KGM	1034:1036	arg1	oligosaccharides					1038:1053	KGM oligosaccharides	1034:1053	KGM oligosaccharides with specific molecular sizes	1034:1083	The findings indicate that KGM oligosaccharides with specific molecular sizes have highly desirable functional properties and potentially could improve gut health by promoting the barrier function of the colonic mucosa.
33587639	2	63	theme	butyrate	715:722	arg1	production					693:702	the production	689:702	the production of colonic butyrate	689:722	The results are the first to reveal that KGMOS regulated intestinal microflora composition to facilitate the production of colonic butyrate.
33587639	3	64	theme	gene	881:884	arg1	Muc2					886:889	the major colonic mucin gene Muc2	857:889	the major colonic mucin gene Muc2	857:889	Elevated butyrate production further increased the acetylation of histone proteins H3 and H4 and thus enhanced the transcription of the major colonic mucin gene Muc2 and the secretion of mucin elements, which represents a new molecular mechanism of KGM oligosaccharide consumption.
33587639	3	65	theme	histone	791:797	arg1	proteins					799:806	histone proteins H3 and H4	791:816	histone proteins H3 and H4	791:816	Elevated butyrate production further increased the acetylation of histone proteins H3 and H4 and thus enhanced the transcription of the major colonic mucin gene Muc2 and the secretion of mucin elements, which represents a new molecular mechanism of KGM oligosaccharide consumption.
33587639	3	65	theme	histone	791:797	arg1	H4					815:816	H4	815:816	H4	815:816	Elevated butyrate production further increased the acetylation of histone proteins H3 and H4 and thus enhanced the transcription of the major colonic mucin gene Muc2 and the secretion of mucin elements, which represents a new molecular mechanism of KGM oligosaccharide consumption.
33587639	3	65	theme	histone	791:797	arg1	H3					808:809	H3	808:809	H3	808:809	Elevated butyrate production further increased the acetylation of histone proteins H3 and H4 and thus enhanced the transcription of the major colonic mucin gene Muc2 and the secretion of mucin elements, which represents a new molecular mechanism of KGM oligosaccharide consumption.
33713445	6	0	theme	%	863:863	arg1	decrease					865:872	a 90% decrease	859:872	a 90% decrease in cellulose in these mutant woods	859:907	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods revealed that the three classes of SCW PtrCesAs are essential for multilayered SCW structure and wood G-fibre.
33713445	9	1	theme	wall	1538:1541	arg1	structures					1543:1552	phloem fibre wall structures	1525:1552	phloem fibre wall structures	1525:1552	Overall, this functional understanding of the SCW PtrCesAs provides further insights into the impact of lacking cellulose biosynthesis on growth, SCW, wood G-fibre and phloem fibre wall structures in the tree.
33713445	4	2	theme	composition	546:556	arg1	evidence					558:565	anatomical, immunohistochemical and wood composition evidence	505:565	anatomical, immunohistochemical and wood composition evidence	505:565	Based on anatomical, immunohistochemical and wood composition evidence, we gained a comprehensive understanding of five SCW PtrCesAs at the genetic level.
33713445	7	3	theme	secondary	1056:1064	arg1	fibres					1073:1078	the mutant primary and secondary phloem fibres	1033:1078	the mutant primary and secondary phloem fibres	1033:1078	In addition, the mutant primary and secondary phloem fibres lost the n(G + L)- and G-layers and retained the thicker S-layers (L, lignified; S, secondary).
33713445	7	4	theme	primary	1044:1050	arg1	fibres					1073:1078	the mutant primary and secondary phloem fibres	1033:1078	the mutant primary and secondary phloem fibres	1033:1078	In addition, the mutant primary and secondary phloem fibres lost the n(G + L)- and G-layers and retained the thicker S-layers (L, lignified; S, secondary).
33713445	2	5	theme	wall	299:302	arg1	CesAs					310:314	secondary cell wall (SCW) CesAs	284:314	secondary cell wall (SCW) CesAs	284:314	However, an overall functional determination of secondary cell wall (SCW) CesAs is still lacking in trees, especially one based on gene knockouts.
33713445	6	6	theme	fibres	848:853	arg1	absence					794:800	The absence	790:800	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods	790:907	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods revealed that the three classes of SCW PtrCesAs are essential for multilayered SCW structure and wood G-fibre.
33713445	6	7	from	fibres	848:853	arg1	woods					903:907	these mutant woods	890:907	these mutant woods	890:907	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods revealed that the three classes of SCW PtrCesAs are essential for multilayered SCW structure and wood G-fibre.
33713445	6	7	from	fibres	848:853	arg1	cellulose					877:885	cellulose	877:885	cellulose	877:885	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods revealed that the three classes of SCW PtrCesAs are essential for multilayered SCW structure and wood G-fibre.
33713445	2	8	theme	secondary	284:292	arg1	wall					299:302	secondary cell wall	284:302	secondary cell wall (SCW) CesAs	284:314	However, an overall functional determination of secondary cell wall (SCW) CesAs is still lacking in trees, especially one based on gene knockouts.
33713445	2	8	theme	secondary	284:292	arg1	SCW					305:307	SCW	305:307	SCW	305:307	However, an overall functional determination of secondary cell wall (SCW) CesAs is still lacking in trees, especially one based on gene knockouts.
33713445	1	9	theme	membrane-localized	182:199	arg1	complex					227:233	a large plasma membrane-localized cellulose synthase (CesA) complex	167:233	a large plasma membrane-localized cellulose synthase (CesA) complex	167:233	Plant cellulose is synthesized by a large plasma membrane-localized cellulose synthase (CesA) complex.
33713445	4	10	theme	genetic	636:642	arg1	level					644:648	the genetic level	632:648	the genetic level	632:648	Based on anatomical, immunohistochemical and wood composition evidence, we gained a comprehensive understanding of five SCW PtrCesAs at the genetic level.
33713445	2	11	theme	CesAs	310:314	arg1	determination					267:279	an overall functional determination	245:279	an overall functional determination of secondary cell wall (SCW) CesAs	245:314	However, an overall functional determination of secondary cell wall (SCW) CesAs is still lacking in trees, especially one based on gene knockouts.
33713445	1	12	theme	synthase	211:218	arg1	complex					227:233	a large plasma membrane-localized cellulose synthase (CesA) complex	167:233	a large plasma membrane-localized cellulose synthase (CesA) complex	167:233	Plant cellulose is synthesized by a large plasma membrane-localized cellulose synthase (CesA) complex.
33713445	9	13	theme	biosynthesis	1479:1490	arg1	impact					1451:1456	the impact	1447:1456	the impact of lacking cellulose biosynthesis on growth, SCW, wood G-fibre and phloem fibre wall structures in the tree	1447:1564	Overall, this functional understanding of the SCW PtrCesAs provides further insights into the impact of lacking cellulose biosynthesis on growth, SCW, wood G-fibre and phloem fibre wall structures in the tree.
33713445	5	14	theme	PtrCesA4	668:675	arg1	loss					660:663	Complete loss	651:663	Complete loss of PtrCesA4, 7A/B or 8A/B	651:689	Complete loss of PtrCesA4, 7A/B or 8A/B led to similar morphological abnormalities, indicating similar and nonredundant genetic functions.
33713445	1	15	theme	CesA	221:224	arg1	complex					227:233	a large plasma membrane-localized cellulose synthase (CesA) complex	167:233	a large plasma membrane-localized cellulose synthase (CesA) complex	167:233	Plant cellulose is synthesized by a large plasma membrane-localized cellulose synthase (CesA) complex.
33713445	6	16	theme	mutant	896:901	arg1	woods					903:907	these mutant woods	890:907	these mutant woods	890:907	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods revealed that the three classes of SCW PtrCesAs are essential for multilayered SCW structure and wood G-fibre.
33713445	8	17	theme	wall	1340:1343	arg1	structures					1345:1354	wood and phloem fibre wall structures	1318:1354	wood and phloem fibre wall structures	1318:1354	Together with polysaccharide immunolocalization data, these findings suggest differences in the role of SCW PtrCesAs-synthesized cellulose in wood and phloem fibre wall structures.
33713445	4	18	theme	SCW	616:618	arg1	PtrCesAs					620:627	five SCW PtrCesAs	611:627	five SCW PtrCesAs	611:627	Based on anatomical, immunohistochemical and wood composition evidence, we gained a comprehensive understanding of five SCW PtrCesAs at the genetic level.
33713445	6	19	from	absence	794:800	arg1	woods					903:907	these mutant woods	890:907	these mutant woods	890:907	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods revealed that the three classes of SCW PtrCesAs are essential for multilayered SCW structure and wood G-fibre.
33713445	6	19	from	absence	794:800	arg1	cellulose					877:885	cellulose	877:885	cellulose	877:885	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods revealed that the three classes of SCW PtrCesAs are essential for multilayered SCW structure and wood G-fibre.
33713445	5	20	theme	7A/B	678:681	arg1	loss					660:663	Complete loss	651:663	Complete loss of PtrCesA4, 7A/B or 8A/B	651:689	Complete loss of PtrCesA4, 7A/B or 8A/B led to similar morphological abnormalities, indicating similar and nonredundant genetic functions.
33713445	7	21	theme	n	1089:1089	arg1	G + L					1091:1095	the n(G + L)-	1085:1097	the n(G + L)-	1085:1097	In addition, the mutant primary and secondary phloem fibres lost the n(G + L)- and G-layers and retained the thicker S-layers (L, lignified; S, secondary).
33713445	1	22	theme	Plant	133:137	arg1	cellulose					139:147	Plant cellulose	133:147	Plant cellulose	133:147	Plant cellulose is synthesized by a large plasma membrane-localized cellulose synthase (CesA) complex.
33713445	9	23	from	structures	1543:1552	arg1	tree					1561:1564	the tree	1557:1564	the tree	1557:1564	Overall, this functional understanding of the SCW PtrCesAs provides further insights into the impact of lacking cellulose biosynthesis on growth, SCW, wood G-fibre and phloem fibre wall structures in the tree.
33713445	9	24	theme	PtrCesAs	1407:1414	arg1	understanding					1382:1394	this functional understanding	1366:1394	this functional understanding of the SCW PtrCesAs	1366:1414	Overall, this functional understanding of the SCW PtrCesAs provides further insights into the impact of lacking cellulose biosynthesis on growth, SCW, wood G-fibre and phloem fibre wall structures in the tree.
33713445	8	25	theme	immunolocalization	1205:1222	arg1	data					1224:1227	polysaccharide immunolocalization data	1190:1227	polysaccharide immunolocalization data	1190:1227	Together with polysaccharide immunolocalization data, these findings suggest differences in the role of SCW PtrCesAs-synthesized cellulose in wood and phloem fibre wall structures.
33713445	5	26	theme	morphological	706:718	arg1	abnormalities					720:732	similar morphological abnormalities	698:732	similar morphological abnormalities	698:732	Complete loss of PtrCesA4, 7A/B or 8A/B led to similar morphological abnormalities, indicating similar and nonredundant genetic functions.
33713445	2	27	theme	overall	248:254	arg1	determination					267:279	an overall functional determination	245:279	an overall functional determination of secondary cell wall (SCW) CesAs	245:314	However, an overall functional determination of secondary cell wall (SCW) CesAs is still lacking in trees, especially one based on gene knockouts.
33713445	9	28	theme	further	1425:1431	arg1	insights					1433:1440	further insights	1425:1440	further insights into the impact of lacking cellulose biosynthesis on growth, SCW, wood G-fibre and phloem fibre wall structures in the tree	1425:1564	Overall, this functional understanding of the SCW PtrCesAs provides further insights into the impact of lacking cellulose biosynthesis on growth, SCW, wood G-fibre and phloem fibre wall structures in the tree.
33713445	6	29	theme	SCW	944:946	arg1	PtrCesAs					948:955	SCW PtrCesAs	944:955	SCW PtrCesAs	944:955	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods revealed that the three classes of SCW PtrCesAs are essential for multilayered SCW structure and wood G-fibre.
33713445	1	30	theme	large	169:173	arg1	complex					227:233	a large plasma membrane-localized cellulose synthase (CesA) complex	167:233	a large plasma membrane-localized cellulose synthase (CesA) complex	167:233	Plant cellulose is synthesized by a large plasma membrane-localized cellulose synthase (CesA) complex.
33713445	5	31	theme	8A/B	686:689	arg1	loss					660:663	Complete loss	651:663	Complete loss of PtrCesA4, 7A/B or 8A/B	651:689	Complete loss of PtrCesA4, 7A/B or 8A/B led to similar morphological abnormalities, indicating similar and nonredundant genetic functions.
33713445	3	32	theme	PtrCesA4	424:431	arg1	knockouts					411:419	the Cas9/gRNA-induced knockouts	389:419	the Cas9/gRNA-induced knockouts of PtrCesA4, 7A, 7B, 8A and 8B genes	389:456	Here, the Cas9/gRNA-induced knockouts of PtrCesA4, 7A, 7B, 8A and 8B genes were produced in Populus trichocarpa.
33713445	0	33	theme	gene	117:120	arg1	knockouts					122:130	the Cas9/gRNA-induced gene knockouts	95:130	the Cas9/gRNA-induced gene knockouts	95:130	Functional understanding of secondary cell wall cellulose synthases in Populus trichocarpa via the Cas9/gRNA-induced gene knockouts.
33713445	9	34	theme	lacking	1461:1467	arg1	biosynthesis					1479:1490	lacking cellulose biosynthesis	1461:1490	lacking cellulose biosynthesis	1461:1490	Overall, this functional understanding of the SCW PtrCesAs provides further insights into the impact of lacking cellulose biosynthesis on growth, SCW, wood G-fibre and phloem fibre wall structures in the tree.
33713445	9	35	from	G-fibre	1513:1519	arg1	tree					1561:1564	the tree	1557:1564	the tree	1557:1564	Overall, this functional understanding of the SCW PtrCesAs provides further insights into the impact of lacking cellulose biosynthesis on growth, SCW, wood G-fibre and phloem fibre wall structures in the tree.
33713445	5	36	theme	similar	698:704	arg1	abnormalities					720:732	similar morphological abnormalities	698:732	similar morphological abnormalities	698:732	Complete loss of PtrCesA4, 7A/B or 8A/B led to similar morphological abnormalities, indicating similar and nonredundant genetic functions.
33713445	2	37	theme	gene	367:370	arg1	knockouts					372:380	gene knockouts	367:380	gene knockouts	367:380	However, an overall functional determination of secondary cell wall (SCW) CesAs is still lacking in trees, especially one based on gene knockouts.
33713445	0	38	theme	Functional	0:9	arg1	understanding					11:23	Functional understanding	0:23	Functional understanding of secondary cell wall cellulose	0:56	Functional understanding of secondary cell wall cellulose synthases in Populus trichocarpa via the Cas9/gRNA-induced gene knockouts.
33713445	7	39	dep	L	1147:1147	arg1	S					1161:1161	S	1161:1161	S	1161:1161	In addition, the mutant primary and secondary phloem fibres lost the n(G + L)- and G-layers and retained the thicker S-layers (L, lignified; S, secondary).
33713445	4	40	theme	anatomical	505:514	arg1	evidence					558:565	anatomical, immunohistochemical and wood composition evidence	505:565	anatomical, immunohistochemical and wood composition evidence	505:565	Based on anatomical, immunohistochemical and wood composition evidence, we gained a comprehensive understanding of five SCW PtrCesAs at the genetic level.
33713445	6	41	from	woods	903:907	arg1	absence					794:800	The absence	790:800	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods	790:907	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods revealed that the three classes of SCW PtrCesAs are essential for multilayered SCW structure and wood G-fibre.
33713445	6	42	theme	layer	824:828	arg1	absence					794:800	The absence	790:800	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods	790:907	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods revealed that the three classes of SCW PtrCesAs are essential for multilayered SCW structure and wood G-fibre.
33713445	0	43	dep	in	68:69	arg1	Populus					71:77	Populus	71:77	Populus	71:77	Functional understanding of secondary cell wall cellulose synthases in Populus trichocarpa via the Cas9/gRNA-induced gene knockouts.
33713445	6	44	theme	SCW	988:990	arg1	structure					992:1000	multilayered SCW structure	975:1000	multilayered SCW structure	975:1000	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods revealed that the three classes of SCW PtrCesAs are essential for multilayered SCW structure and wood G-fibre.
33713445	4	45	theme	immunohistochemical	517:535	arg1	evidence					558:565	anatomical, immunohistochemical and wood composition evidence	505:565	anatomical, immunohistochemical and wood composition evidence	505:565	Based on anatomical, immunohistochemical and wood composition evidence, we gained a comprehensive understanding of five SCW PtrCesAs at the genetic level.
33713445	8	46	theme	PtrCesAs-synthesized	1284:1303	arg1	cellulose					1305:1313	SCW PtrCesAs-synthesized cellulose	1280:1313	SCW PtrCesAs-synthesized cellulose	1280:1313	Together with polysaccharide immunolocalization data, these findings suggest differences in the role of SCW PtrCesAs-synthesized cellulose in wood and phloem fibre wall structures.
33713445	0	47	theme	cell	38:41	arg1	cellulose					48:56	secondary cell wall cellulose	28:56	secondary cell wall cellulose	28:56	Functional understanding of secondary cell wall cellulose synthases in Populus trichocarpa via the Cas9/gRNA-induced gene knockouts.
33713445	5	48	theme	similar	746:752	arg1	functions					779:787	similar and nonredundant genetic functions	746:787	similar and nonredundant genetic functions	746:787	Complete loss of PtrCesA4, 7A/B or 8A/B led to similar morphological abnormalities, indicating similar and nonredundant genetic functions.
33713445	6	49	theme	G	821:821	arg1	layer					824:828	the gelatinous (G) layer	805:828	the gelatinous (G) layer	805:828	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods revealed that the three classes of SCW PtrCesAs are essential for multilayered SCW structure and wood G-fibre.
33713445	6	50	from	cellulose	877:885	arg1	absence					794:800	The absence	790:800	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods	790:907	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods revealed that the three classes of SCW PtrCesAs are essential for multilayered SCW structure and wood G-fibre.
33713445	0	51	theme	in	68:69	arg1	trichocarpa					79:89	in Populus trichocarpa	68:89	in Populus trichocarpa	68:89	Functional understanding of secondary cell wall cellulose synthases in Populus trichocarpa via the Cas9/gRNA-induced gene knockouts.
33713445	3	52	theme	8B	449:450	arg1	PtrCesA4					424:431	PtrCesA4	424:431	PtrCesA4	424:431	Here, the Cas9/gRNA-induced knockouts of PtrCesA4, 7A, 7B, 8A and 8B genes were produced in Populus trichocarpa.
33713445	3	52	theme	8B	449:450	arg1	genes					452:456	8B genes	449:456	8B genes	449:456	Here, the Cas9/gRNA-induced knockouts of PtrCesA4, 7A, 7B, 8A and 8B genes were produced in Populus trichocarpa.
33713445	5	53	theme	nonredundant	758:769	arg1	functions					779:787	similar and nonredundant genetic functions	746:787	similar and nonredundant genetic functions	746:787	Complete loss of PtrCesA4, 7A/B or 8A/B led to similar morphological abnormalities, indicating similar and nonredundant genetic functions.
33713445	6	54	theme	gelatinous	809:818	arg1	layer					824:828	the gelatinous (G) layer	805:828	the gelatinous (G) layer	805:828	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods revealed that the three classes of SCW PtrCesAs are essential for multilayered SCW structure and wood G-fibre.
33713445	9	55	theme	wood	1508:1511	arg1	G-fibre					1513:1519	wood G-fibre	1508:1519	wood G-fibre	1508:1519	Overall, this functional understanding of the SCW PtrCesAs provides further insights into the impact of lacking cellulose biosynthesis on growth, SCW, wood G-fibre and phloem fibre wall structures in the tree.
33713445	8	56	theme	fibre	1334:1338	arg1	structures					1345:1354	wood and phloem fibre wall structures	1318:1354	wood and phloem fibre wall structures	1318:1354	Together with polysaccharide immunolocalization data, these findings suggest differences in the role of SCW PtrCesAs-synthesized cellulose in wood and phloem fibre wall structures.
33713445	6	57	theme	decrease	865:872	arg1	absence					794:800	The absence	790:800	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods	790:907	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods revealed that the three classes of SCW PtrCesAs are essential for multilayered SCW structure and wood G-fibre.
33713445	9	58	theme	fibre	1532:1536	arg1	structures					1543:1552	phloem fibre wall structures	1525:1552	phloem fibre wall structures	1525:1552	Overall, this functional understanding of the SCW PtrCesAs provides further insights into the impact of lacking cellulose biosynthesis on growth, SCW, wood G-fibre and phloem fibre wall structures in the tree.
33713445	6	59	from	decrease	865:872	arg1	woods					903:907	these mutant woods	890:907	these mutant woods	890:907	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods revealed that the three classes of SCW PtrCesAs are essential for multilayered SCW structure and wood G-fibre.
33713445	6	59	from	decrease	865:872	arg1	cellulose					877:885	cellulose	877:885	cellulose	877:885	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods revealed that the three classes of SCW PtrCesAs are essential for multilayered SCW structure and wood G-fibre.
33713445	8	60	from	role	1272:1275	arg1	structures					1345:1354	wood and phloem fibre wall structures	1318:1354	wood and phloem fibre wall structures	1318:1354	Together with polysaccharide immunolocalization data, these findings suggest differences in the role of SCW PtrCesAs-synthesized cellulose in wood and phloem fibre wall structures.
33713445	6	61	theme	90	861:862	arg1	%					863:863	%	863:863	%	863:863	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods revealed that the three classes of SCW PtrCesAs are essential for multilayered SCW structure and wood G-fibre.
33713445	4	62	theme	wood	541:544	arg1	evidence					558:565	anatomical, immunohistochemical and wood composition evidence	505:565	anatomical, immunohistochemical and wood composition evidence	505:565	Based on anatomical, immunohistochemical and wood composition evidence, we gained a comprehensive understanding of five SCW PtrCesAs at the genetic level.
33713445	7	63	theme	mutant	1037:1042	arg1	fibres					1073:1078	the mutant primary and secondary phloem fibres	1033:1078	the mutant primary and secondary phloem fibres	1033:1078	In addition, the mutant primary and secondary phloem fibres lost the n(G + L)- and G-layers and retained the thicker S-layers (L, lignified; S, secondary).
33713445	2	64	theme	cell	294:297	arg1	wall					299:302	secondary cell wall	284:302	secondary cell wall (SCW) CesAs	284:314	However, an overall functional determination of secondary cell wall (SCW) CesAs is still lacking in trees, especially one based on gene knockouts.
33713445	2	64	theme	cell	294:297	arg1	SCW					305:307	SCW	305:307	SCW	305:307	However, an overall functional determination of secondary cell wall (SCW) CesAs is still lacking in trees, especially one based on gene knockouts.
33713445	1	65	theme	plasma	175:180	arg1	complex					227:233	a large plasma membrane-localized cellulose synthase (CesA) complex	167:233	a large plasma membrane-localized cellulose synthase (CesA) complex	167:233	Plant cellulose is synthesized by a large plasma membrane-localized cellulose synthase (CesA) complex.
33713445	0	66	theme	cellulose	48:56	arg1	understanding					11:23	Functional understanding	0:23	Functional understanding of secondary cell wall cellulose	0:56	Functional understanding of secondary cell wall cellulose synthases in Populus trichocarpa via the Cas9/gRNA-induced gene knockouts.
33713445	6	67	theme	one-layer-walled	831:846	arg1	fibres					848:853	one-layer-walled fibres	831:853	one-layer-walled fibres	831:853	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods revealed that the three classes of SCW PtrCesAs are essential for multilayered SCW structure and wood G-fibre.
33713445	4	68	theme	PtrCesAs	620:627	arg1	understanding					594:606	a comprehensive understanding	578:606	a comprehensive understanding of five SCW PtrCesAs	578:627	Based on anatomical, immunohistochemical and wood composition evidence, we gained a comprehensive understanding of five SCW PtrCesAs at the genetic level.
33713445	1	69	theme	cellulose	201:209	arg1	complex					227:233	a large plasma membrane-localized cellulose synthase (CesA) complex	167:233	a large plasma membrane-localized cellulose synthase (CesA) complex	167:233	Plant cellulose is synthesized by a large plasma membrane-localized cellulose synthase (CesA) complex.
33713445	6	70	from	layer	824:828	arg1	woods					903:907	these mutant woods	890:907	these mutant woods	890:907	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods revealed that the three classes of SCW PtrCesAs are essential for multilayered SCW structure and wood G-fibre.
33713445	6	70	from	layer	824:828	arg1	cellulose					877:885	cellulose	877:885	cellulose	877:885	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods revealed that the three classes of SCW PtrCesAs are essential for multilayered SCW structure and wood G-fibre.
33713445	9	71	theme	functional	1371:1380	arg1	understanding					1382:1394	this functional understanding	1366:1394	this functional understanding of the SCW PtrCesAs	1366:1414	Overall, this functional understanding of the SCW PtrCesAs provides further insights into the impact of lacking cellulose biosynthesis on growth, SCW, wood G-fibre and phloem fibre wall structures in the tree.
33713445	5	72	theme	Complete	651:658	arg1	loss					660:663	Complete loss	651:663	Complete loss of PtrCesA4, 7A/B or 8A/B	651:689	Complete loss of PtrCesA4, 7A/B or 8A/B led to similar morphological abnormalities, indicating similar and nonredundant genetic functions.
33713445	9	73	theme	SCW	1403:1405	arg1	PtrCesAs					1407:1414	the SCW PtrCesAs	1399:1414	the SCW PtrCesAs	1399:1414	Overall, this functional understanding of the SCW PtrCesAs provides further insights into the impact of lacking cellulose biosynthesis on growth, SCW, wood G-fibre and phloem fibre wall structures in the tree.
33713445	9	74	from	growth	1495:1500	arg1	tree					1561:1564	the tree	1557:1564	the tree	1557:1564	Overall, this functional understanding of the SCW PtrCesAs provides further insights into the impact of lacking cellulose biosynthesis on growth, SCW, wood G-fibre and phloem fibre wall structures in the tree.
33713445	7	75	theme	thicker	1129:1135	arg1	S-layers					1137:1144	the thicker S-layers	1125:1144	the thicker S-layers (L, lignified; S, secondary)	1125:1173	In addition, the mutant primary and secondary phloem fibres lost the n(G + L)- and G-layers and retained the thicker S-layers (L, lignified; S, secondary).
33713445	2	76	theme	functional	256:265	arg1	determination					267:279	an overall functional determination	245:279	an overall functional determination of secondary cell wall (SCW) CesAs	245:314	However, an overall functional determination of secondary cell wall (SCW) CesAs is still lacking in trees, especially one based on gene knockouts.
33713445	6	77	theme	PtrCesAs	948:955	arg1	classes					933:939	the three classes	923:939	the three classes of SCW PtrCesAs	923:955	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods revealed that the three classes of SCW PtrCesAs are essential for multilayered SCW structure and wood G-fibre.
33713445	6	77	theme	PtrCesAs	948:955	arg1	essential					961:969	essential	961:969	essential	961:969	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods revealed that the three classes of SCW PtrCesAs are essential for multilayered SCW structure and wood G-fibre.
33713445	8	78	theme	polysaccharide	1190:1203	arg1	data					1224:1227	polysaccharide immunolocalization data	1190:1227	polysaccharide immunolocalization data	1190:1227	Together with polysaccharide immunolocalization data, these findings suggest differences in the role of SCW PtrCesAs-synthesized cellulose in wood and phloem fibre wall structures.
33713445	0	79	theme	Cas9/gRNA-induced	99:115	arg1	knockouts					122:130	the Cas9/gRNA-induced gene knockouts	95:130	the Cas9/gRNA-induced gene knockouts	95:130	Functional understanding of secondary cell wall cellulose synthases in Populus trichocarpa via the Cas9/gRNA-induced gene knockouts.
33713445	9	80	from	impact	1451:1456	arg1	structures					1543:1552	phloem fibre wall structures	1525:1552	phloem fibre wall structures	1525:1552	Overall, this functional understanding of the SCW PtrCesAs provides further insights into the impact of lacking cellulose biosynthesis on growth, SCW, wood G-fibre and phloem fibre wall structures in the tree.
33713445	9	80	from	impact	1451:1456	arg1	SCW					1503:1505	SCW	1503:1505	SCW	1503:1505	Overall, this functional understanding of the SCW PtrCesAs provides further insights into the impact of lacking cellulose biosynthesis on growth, SCW, wood G-fibre and phloem fibre wall structures in the tree.
33713445	9	80	from	impact	1451:1456	arg1	G-fibre					1513:1519	wood G-fibre	1508:1519	wood G-fibre	1508:1519	Overall, this functional understanding of the SCW PtrCesAs provides further insights into the impact of lacking cellulose biosynthesis on growth, SCW, wood G-fibre and phloem fibre wall structures in the tree.
33713445	9	80	from	impact	1451:1456	arg1	growth					1495:1500	growth	1495:1500	growth	1495:1500	Overall, this functional understanding of the SCW PtrCesAs provides further insights into the impact of lacking cellulose biosynthesis on growth, SCW, wood G-fibre and phloem fibre wall structures in the tree.
33713445	5	81	theme	genetic	771:777	arg1	functions					779:787	similar and nonredundant genetic functions	746:787	similar and nonredundant genetic functions	746:787	Complete loss of PtrCesA4, 7A/B or 8A/B led to similar morphological abnormalities, indicating similar and nonredundant genetic functions.
33713445	9	82	theme	cellulose	1469:1477	arg1	biosynthesis					1479:1490	lacking cellulose biosynthesis	1461:1490	lacking cellulose biosynthesis	1461:1490	Overall, this functional understanding of the SCW PtrCesAs provides further insights into the impact of lacking cellulose biosynthesis on growth, SCW, wood G-fibre and phloem fibre wall structures in the tree.
33713445	6	83	theme	wood	1006:1009	arg1	G-fibre					1011:1017	wood G-fibre	1006:1017	wood G-fibre	1006:1017	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods revealed that the three classes of SCW PtrCesAs are essential for multilayered SCW structure and wood G-fibre.
33713445	0	84	theme	secondary	28:36	arg1	cellulose					48:56	secondary cell wall cellulose	28:56	secondary cell wall cellulose	28:56	Functional understanding of secondary cell wall cellulose synthases in Populus trichocarpa via the Cas9/gRNA-induced gene knockouts.
33713445	0	85	theme	wall	43:46	arg1	cellulose					48:56	secondary cell wall cellulose	28:56	secondary cell wall cellulose	28:56	Functional understanding of secondary cell wall cellulose synthases in Populus trichocarpa via the Cas9/gRNA-induced gene knockouts.
33713445	9	86	from	SCW	1503:1505	arg1	tree					1561:1564	the tree	1557:1564	the tree	1557:1564	Overall, this functional understanding of the SCW PtrCesAs provides further insights into the impact of lacking cellulose biosynthesis on growth, SCW, wood G-fibre and phloem fibre wall structures in the tree.
33713445	6	87	theme	multilayered	975:986	arg1	structure					992:1000	multilayered SCW structure	975:1000	multilayered SCW structure	975:1000	The absence of the gelatinous (G) layer, one-layer-walled fibres and a 90% decrease in cellulose in these mutant woods revealed that the three classes of SCW PtrCesAs are essential for multilayered SCW structure and wood G-fibre.
33713445	3	88	theme	Cas9/gRNA-induced	393:409	arg1	knockouts					411:419	the Cas9/gRNA-induced knockouts	389:419	the Cas9/gRNA-induced knockouts of PtrCesA4, 7A, 7B, 8A and 8B genes	389:456	Here, the Cas9/gRNA-induced knockouts of PtrCesA4, 7A, 7B, 8A and 8B genes were produced in Populus trichocarpa.
33713445	7	89	dep	S-layers	1137:1144	arg1	lignified					1150:1158	lignified	1150:1158	lignified	1150:1158	In addition, the mutant primary and secondary phloem fibres lost the n(G + L)- and G-layers and retained the thicker S-layers (L, lignified; S, secondary).
33713445	7	89	dep	S-layers	1137:1144	arg1	L					1147:1147	L	1147:1147	L	1147:1147	In addition, the mutant primary and secondary phloem fibres lost the n(G + L)- and G-layers and retained the thicker S-layers (L, lignified; S, secondary).
33713445	7	89	dep	S-layers	1137:1144	arg1	secondary					1164:1172	secondary	1164:1172	secondary	1164:1172	In addition, the mutant primary and secondary phloem fibres lost the n(G + L)- and G-layers and retained the thicker S-layers (L, lignified; S, secondary).
33713445	8	90	theme	SCW	1280:1282	arg1	cellulose					1305:1313	SCW PtrCesAs-synthesized cellulose	1280:1313	SCW PtrCesAs-synthesized cellulose	1280:1313	Together with polysaccharide immunolocalization data, these findings suggest differences in the role of SCW PtrCesAs-synthesized cellulose in wood and phloem fibre wall structures.
33713445	7	91	theme	phloem	1066:1071	arg1	fibres					1073:1078	the mutant primary and secondary phloem fibres	1033:1078	the mutant primary and secondary phloem fibres	1033:1078	In addition, the mutant primary and secondary phloem fibres lost the n(G + L)- and G-layers and retained the thicker S-layers (L, lignified; S, secondary).
33713445	8	92	theme	cellulose	1305:1313	arg1	role					1272:1275	the role	1268:1275	the role of SCW PtrCesAs-synthesized cellulose in wood and phloem fibre wall structures	1268:1354	Together with polysaccharide immunolocalization data, these findings suggest differences in the role of SCW PtrCesAs-synthesized cellulose in wood and phloem fibre wall structures.
33713445	8	93	from	differences	1253:1263	arg1	role					1272:1275	the role	1268:1275	the role of SCW PtrCesAs-synthesized cellulose in wood and phloem fibre wall structures	1268:1354	Together with polysaccharide immunolocalization data, these findings suggest differences in the role of SCW PtrCesAs-synthesized cellulose in wood and phloem fibre wall structures.
33713445	8	94	theme	wood	1318:1321	arg1	structures					1345:1354	wood and phloem fibre wall structures	1318:1354	wood and phloem fibre wall structures	1318:1354	Together with polysaccharide immunolocalization data, these findings suggest differences in the role of SCW PtrCesAs-synthesized cellulose in wood and phloem fibre wall structures.
33713445	9	95	theme	phloem	1525:1530	arg1	structures					1543:1552	phloem fibre wall structures	1525:1552	phloem fibre wall structures	1525:1552	Overall, this functional understanding of the SCW PtrCesAs provides further insights into the impact of lacking cellulose biosynthesis on growth, SCW, wood G-fibre and phloem fibre wall structures in the tree.
33713445	4	96	theme	comprehensive	580:592	arg1	understanding					594:606	a comprehensive understanding	578:606	a comprehensive understanding of five SCW PtrCesAs	578:627	Based on anatomical, immunohistochemical and wood composition evidence, we gained a comprehensive understanding of five SCW PtrCesAs at the genetic level.
33713445	8	97	theme	phloem	1327:1332	arg1	structures					1345:1354	wood and phloem fibre wall structures	1318:1354	wood and phloem fibre wall structures	1318:1354	Together with polysaccharide immunolocalization data, these findings suggest differences in the role of SCW PtrCesAs-synthesized cellulose in wood and phloem fibre wall structures.
33713445	3	98	theme	Populus	475:481	arg1	trichocarpa					483:493	Populus trichocarpa	475:493	Populus trichocarpa	475:493	Here, the Cas9/gRNA-induced knockouts of PtrCesA4, 7A, 7B, 8A and 8B genes were produced in Populus trichocarpa.
32107309	3	0	theme	kinetic	752:758	arg1	assays					776:781	kinetic and biochemical assays	752:781	kinetic and biochemical assays	752:781	Herein, using recombinantly expressed mycobacterial protein, bioinformatics analyses, and kinetic and biochemical assays, we demonstrate that the AG can be remodeled by a mycobacterial endogenous enzyme.
32107309	4	1	theme	mycobacterial	899:911	arg1	protein					928:934	the mycobacterial GlfH1 (Rv3096) protein	895:934	the mycobacterial GlfH1 (Rv3096) protein	895:934	In particular, we found that the mycobacterial GlfH1 (Rv3096) protein exhibits exo-β-d-galactofuranose hydrolase activity and is capable of hydrolyzing the galactan chain of AG by recurrent cleavage of the terminal β-(1,5) and β-(1,6)-Galf linkages.
32107309	4	1	theme	mycobacterial	899:911	arg1	capable					995:1001	capable	995:1001	capable	995:1001	In particular, we found that the mycobacterial GlfH1 (Rv3096) protein exhibits exo-β-d-galactofuranose hydrolase activity and is capable of hydrolyzing the galactan chain of AG by recurrent cleavage of the terminal β-(1,5) and β-(1,6)-Galf linkages.
32107309	1	2	theme	mAGP	201:204	arg1	biogenesis					207:216	mycolylarabinogalactan-peptidoglycan (mAGP) biogenesis	163:216	mycolylarabinogalactan-peptidoglycan (mAGP) biogenesis	163:216	Despite impressive progress made over the past 20 years in our understanding of mycolylarabinogalactan-peptidoglycan (mAGP) biogenesis, the mechanisms by which the tubercle bacillus Mycobacterium tuberculosis adapts its cell wall structure and composition to various environmental conditions, especially during infection, remain poorly understood.
32107309	5	3	theme	mycobacterial	1222:1234	arg1	AG					1236:1237	mycobacterial AG	1222:1237	mycobacterial AG	1222:1237	The characterization of this galactosidase represents a first step toward understanding the remodeling of mycobacterial AG.
32107309	2	4	theme	envelope	559:566	arg1	constituent					521:531	the constituent	517:531	the constituent of the mycobacterial cell envelope that undergoes the least structural changes	517:610	Being the central portion of the mAGP complex, arabinogalactan (AG) is believed to be the constituent of the mycobacterial cell envelope that undergoes the least structural changes, but no reports exist supporting this assumption.
32107309	2	4	theme	envelope	559:566	arg1	arabinogalactan					478:492	arabinogalactan	478:492	arabinogalactan (AG)	478:497	Being the central portion of the mAGP complex, arabinogalactan (AG) is believed to be the constituent of the mycobacterial cell envelope that undergoes the least structural changes, but no reports exist supporting this assumption.
32107309	5	5	theme	galactosidase	1145:1157	arg1	characterization					1120:1135	The characterization	1116:1135	The characterization of this galactosidase	1116:1157	The characterization of this galactosidase represents a first step toward understanding the remodeling of mycobacterial AG.
32107309	1	6	theme	cell	303:306	arg1	structure					313:321	cell wall structure	303:321	cell wall structure	303:321	Despite impressive progress made over the past 20 years in our understanding of mycolylarabinogalactan-peptidoglycan (mAGP) biogenesis, the mechanisms by which the tubercle bacillus Mycobacterium tuberculosis adapts its cell wall structure and composition to various environmental conditions, especially during infection, remain poorly understood.
32107309	2	7	theme	cell	554:557	arg1	envelope					559:566	the mycobacterial cell envelope	536:566	the mycobacterial cell envelope that undergoes the least structural changes	536:610	Being the central portion of the mAGP complex, arabinogalactan (AG) is believed to be the constituent of the mycobacterial cell envelope that undergoes the least structural changes, but no reports exist supporting this assumption.
32107309	4	8	theme	GlfH1	913:917	arg1	protein					928:934	the mycobacterial GlfH1 (Rv3096) protein	895:934	the mycobacterial GlfH1 (Rv3096) protein	895:934	In particular, we found that the mycobacterial GlfH1 (Rv3096) protein exhibits exo-β-d-galactofuranose hydrolase activity and is capable of hydrolyzing the galactan chain of AG by recurrent cleavage of the terminal β-(1,5) and β-(1,6)-Galf linkages.
32107309	4	8	theme	GlfH1	913:917	arg1	capable					995:1001	capable	995:1001	capable	995:1001	In particular, we found that the mycobacterial GlfH1 (Rv3096) protein exhibits exo-β-d-galactofuranose hydrolase activity and is capable of hydrolyzing the galactan chain of AG by recurrent cleavage of the terminal β-(1,5) and β-(1,6)-Galf linkages.
32107309	1	9	theme	impressive	91:100	arg1	progress					102:109	impressive progress	91:109	impressive progress made over the past 20 years in our understanding of mycolylarabinogalactan-peptidoglycan (mAGP) biogenesis	91:216	Despite impressive progress made over the past 20 years in our understanding of mycolylarabinogalactan-peptidoglycan (mAGP) biogenesis, the mechanisms by which the tubercle bacillus Mycobacterium tuberculosis adapts its cell wall structure and composition to various environmental conditions, especially during infection, remain poorly understood.
32107309	1	10	theme	wall	308:311	arg1	structure					313:321	cell wall structure	303:321	cell wall structure	303:321	Despite impressive progress made over the past 20 years in our understanding of mycolylarabinogalactan-peptidoglycan (mAGP) biogenesis, the mechanisms by which the tubercle bacillus Mycobacterium tuberculosis adapts its cell wall structure and composition to various environmental conditions, especially during infection, remain poorly understood.
32107309	2	11	theme	mycobacterial	540:552	arg1	envelope					559:566	the mycobacterial cell envelope	536:566	the mycobacterial cell envelope that undergoes the least structural changes	536:610	Being the central portion of the mAGP complex, arabinogalactan (AG) is believed to be the constituent of the mycobacterial cell envelope that undergoes the least structural changes, but no reports exist supporting this assumption.
32107309	4	12	dep	β-	1081:1082	arg1	linkages					1106:1113	-Galf linkages	1100:1113	-Galf linkages	1100:1113	In particular, we found that the mycobacterial GlfH1 (Rv3096) protein exhibits exo-β-d-galactofuranose hydrolase activity and is capable of hydrolyzing the galactan chain of AG by recurrent cleavage of the terminal β-(1,5) and β-(1,6)-Galf linkages.
32107309	1	13	theme	biogenesis	207:216	arg1	understanding					146:158	our understanding	142:158	our understanding of mycolylarabinogalactan-peptidoglycan (mAGP) biogenesis	142:216	Despite impressive progress made over the past 20 years in our understanding of mycolylarabinogalactan-peptidoglycan (mAGP) biogenesis, the mechanisms by which the tubercle bacillus Mycobacterium tuberculosis adapts its cell wall structure and composition to various environmental conditions, especially during infection, remain poorly understood.
32107309	0	14	theme	endogenous	4:13	arg1	GlfH1					35:39	The endogenous galactofuranosidase GlfH1	0:39	The endogenous galactofuranosidase GlfH1	0:39	The endogenous galactofuranosidase GlfH1 hydrolyzes mycobacterial arabinogalactan.
32107309	3	15	theme	biochemical	764:774	arg1	assays					776:781	kinetic and biochemical assays	752:781	kinetic and biochemical assays	752:781	Herein, using recombinantly expressed mycobacterial protein, bioinformatics analyses, and kinetic and biochemical assays, we demonstrate that the AG can be remodeled by a mycobacterial endogenous enzyme.
32107309	4	16	theme	Rv3096	920:925	arg1	protein					928:934	the mycobacterial GlfH1 (Rv3096) protein	895:934	the mycobacterial GlfH1 (Rv3096) protein	895:934	In particular, we found that the mycobacterial GlfH1 (Rv3096) protein exhibits exo-β-d-galactofuranose hydrolase activity and is capable of hydrolyzing the galactan chain of AG by recurrent cleavage of the terminal β-(1,5) and β-(1,6)-Galf linkages.
32107309	4	16	theme	Rv3096	920:925	arg1	capable					995:1001	capable	995:1001	capable	995:1001	In particular, we found that the mycobacterial GlfH1 (Rv3096) protein exhibits exo-β-d-galactofuranose hydrolase activity and is capable of hydrolyzing the galactan chain of AG by recurrent cleavage of the terminal β-(1,5) and β-(1,6)-Galf linkages.
32107309	1	17	from	years	133:137	arg1	understanding					146:158	our understanding	142:158	our understanding of mycolylarabinogalactan-peptidoglycan (mAGP) biogenesis	142:216	Despite impressive progress made over the past 20 years in our understanding of mycolylarabinogalactan-peptidoglycan (mAGP) biogenesis, the mechanisms by which the tubercle bacillus Mycobacterium tuberculosis adapts its cell wall structure and composition to various environmental conditions, especially during infection, remain poorly understood.
32107309	4	18	theme	β-	1081:1082	arg1	cleavage					1056:1063	recurrent cleavage	1046:1063	recurrent cleavage of the terminal β-(1,5) and β-(1,6)-Galf linkages	1046:1113	In particular, we found that the mycobacterial GlfH1 (Rv3096) protein exhibits exo-β-d-galactofuranose hydrolase activity and is capable of hydrolyzing the galactan chain of AG by recurrent cleavage of the terminal β-(1,5) and β-(1,6)-Galf linkages.
32107309	2	19	theme	least	587:591	arg1	changes					604:610	the least structural changes	583:610	the least structural changes	583:610	Being the central portion of the mAGP complex, arabinogalactan (AG) is believed to be the constituent of the mycobacterial cell envelope that undergoes the least structural changes, but no reports exist supporting this assumption.
32107309	1	20	theme	past	125:128	arg1	years					133:137	the past 20 years	121:137	the past 20 years in our understanding of mycolylarabinogalactan-peptidoglycan (mAGP) biogenesis	121:216	Despite impressive progress made over the past 20 years in our understanding of mycolylarabinogalactan-peptidoglycan (mAGP) biogenesis, the mechanisms by which the tubercle bacillus Mycobacterium tuberculosis adapts its cell wall structure and composition to various environmental conditions, especially during infection, remain poorly understood.
32107309	4	21	theme	-Galf	1100:1104	arg1	linkages					1106:1113	-Galf linkages	1100:1113	-Galf linkages	1100:1113	In particular, we found that the mycobacterial GlfH1 (Rv3096) protein exhibits exo-β-d-galactofuranose hydrolase activity and is capable of hydrolyzing the galactan chain of AG by recurrent cleavage of the terminal β-(1,5) and β-(1,6)-Galf linkages.
32107309	2	22	theme	complex	469:475	arg1	portion					449:455	the central portion	437:455	the central portion of the mAGP complex	437:475	Being the central portion of the mAGP complex, arabinogalactan (AG) is believed to be the constituent of the mycobacterial cell envelope that undergoes the least structural changes, but no reports exist supporting this assumption.
32107309	0	23	theme	galactofuranosidase	15:33	arg1	GlfH1					35:39	The endogenous galactofuranosidase GlfH1	0:39	The endogenous galactofuranosidase GlfH1	0:39	The endogenous galactofuranosidase GlfH1 hydrolyzes mycobacterial arabinogalactan.
32107309	4	24	theme	exo-β-d-galactofuranose	945:967	arg1	activity					979:986	exo-β-d-galactofuranose hydrolase activity	945:986	exo-β-d-galactofuranose hydrolase activity	945:986	In particular, we found that the mycobacterial GlfH1 (Rv3096) protein exhibits exo-β-d-galactofuranose hydrolase activity and is capable of hydrolyzing the galactan chain of AG by recurrent cleavage of the terminal β-(1,5) and β-(1,6)-Galf linkages.
32107309	2	25	theme	mAGP	464:467	arg1	complex					469:475	the mAGP complex	460:475	the mAGP complex	460:475	Being the central portion of the mAGP complex, arabinogalactan (AG) is believed to be the constituent of the mycobacterial cell envelope that undergoes the least structural changes, but no reports exist supporting this assumption.
32107309	0	26	theme	mycobacterial	52:64	arg1	arabinogalactan					66:80	mycobacterial arabinogalactan	52:80	mycobacterial arabinogalactan	52:80	The endogenous galactofuranosidase GlfH1 hydrolyzes mycobacterial arabinogalactan.
32107309	3	27	theme	expressed	690:698	arg1	protein					714:720	recombinantly expressed mycobacterial protein	676:720	recombinantly expressed mycobacterial protein	676:720	Herein, using recombinantly expressed mycobacterial protein, bioinformatics analyses, and kinetic and biochemical assays, we demonstrate that the AG can be remodeled by a mycobacterial endogenous enzyme.
32107309	4	28	theme	AG	1040:1041	arg1	chain					1031:1035	the galactan chain	1018:1035	the galactan chain of AG	1018:1041	In particular, we found that the mycobacterial GlfH1 (Rv3096) protein exhibits exo-β-d-galactofuranose hydrolase activity and is capable of hydrolyzing the galactan chain of AG by recurrent cleavage of the terminal β-(1,5) and β-(1,6)-Galf linkages.
32107309	1	29	theme	various	342:348	arg1	conditions					364:373	various environmental conditions	342:373	various environmental conditions	342:373	Despite impressive progress made over the past 20 years in our understanding of mycolylarabinogalactan-peptidoglycan (mAGP) biogenesis, the mechanisms by which the tubercle bacillus Mycobacterium tuberculosis adapts its cell wall structure and composition to various environmental conditions, especially during infection, remain poorly understood.
32107309	1	30	theme	environmental	350:362	arg1	conditions					364:373	various environmental conditions	342:373	various environmental conditions	342:373	Despite impressive progress made over the past 20 years in our understanding of mycolylarabinogalactan-peptidoglycan (mAGP) biogenesis, the mechanisms by which the tubercle bacillus Mycobacterium tuberculosis adapts its cell wall structure and composition to various environmental conditions, especially during infection, remain poorly understood.
32107309	4	31	theme	recurrent	1046:1054	arg1	cleavage					1056:1063	recurrent cleavage	1046:1063	recurrent cleavage of the terminal β-(1,5) and β-(1,6)-Galf linkages	1046:1113	In particular, we found that the mycobacterial GlfH1 (Rv3096) protein exhibits exo-β-d-galactofuranose hydrolase activity and is capable of hydrolyzing the galactan chain of AG by recurrent cleavage of the terminal β-(1,5) and β-(1,6)-Galf linkages.
32107309	5	32	theme	first	1172:1176	arg1	step					1178:1181	a first step	1170:1181	a first step toward understanding the remodeling of mycobacterial AG	1170:1237	The characterization of this galactosidase represents a first step toward understanding the remodeling of mycobacterial AG.
32107309	4	33	theme	hydrolase	969:977	arg1	activity					979:986	exo-β-d-galactofuranose hydrolase activity	945:986	exo-β-d-galactofuranose hydrolase activity	945:986	In particular, we found that the mycobacterial GlfH1 (Rv3096) protein exhibits exo-β-d-galactofuranose hydrolase activity and is capable of hydrolyzing the galactan chain of AG by recurrent cleavage of the terminal β-(1,5) and β-(1,6)-Galf linkages.
32107309	3	34	theme	bioinformatics	723:736	arg1	analyses					738:745	bioinformatics analyses	723:745	bioinformatics analyses	723:745	Herein, using recombinantly expressed mycobacterial protein, bioinformatics analyses, and kinetic and biochemical assays, we demonstrate that the AG can be remodeled by a mycobacterial endogenous enzyme.
32107309	4	35	theme	β-	1093:1094	arg1	cleavage					1056:1063	recurrent cleavage	1046:1063	recurrent cleavage of the terminal β-(1,5) and β-(1,6)-Galf linkages	1046:1113	In particular, we found that the mycobacterial GlfH1 (Rv3096) protein exhibits exo-β-d-galactofuranose hydrolase activity and is capable of hydrolyzing the galactan chain of AG by recurrent cleavage of the terminal β-(1,5) and β-(1,6)-Galf linkages.
32107309	3	36	theme	mycobacterial	833:845	arg1	enzyme					858:863	a mycobacterial endogenous enzyme	831:863	a mycobacterial endogenous enzyme	831:863	Herein, using recombinantly expressed mycobacterial protein, bioinformatics analyses, and kinetic and biochemical assays, we demonstrate that the AG can be remodeled by a mycobacterial endogenous enzyme.
32107309	2	37	theme	structural	593:602	arg1	changes					604:610	the least structural changes	583:610	the least structural changes	583:610	Being the central portion of the mAGP complex, arabinogalactan (AG) is believed to be the constituent of the mycobacterial cell envelope that undergoes the least structural changes, but no reports exist supporting this assumption.
32107309	3	38	theme	mycobacterial	700:712	arg1	protein					714:720	recombinantly expressed mycobacterial protein	676:720	recombinantly expressed mycobacterial protein	676:720	Herein, using recombinantly expressed mycobacterial protein, bioinformatics analyses, and kinetic and biochemical assays, we demonstrate that the AG can be remodeled by a mycobacterial endogenous enzyme.
32107309	3	39	theme	endogenous	847:856	arg1	enzyme					858:863	a mycobacterial endogenous enzyme	831:863	a mycobacterial endogenous enzyme	831:863	Herein, using recombinantly expressed mycobacterial protein, bioinformatics analyses, and kinetic and biochemical assays, we demonstrate that the AG can be remodeled by a mycobacterial endogenous enzyme.
32107309	4	40	theme	galactan	1022:1029	arg1	chain					1031:1035	the galactan chain	1018:1035	the galactan chain of AG	1018:1041	In particular, we found that the mycobacterial GlfH1 (Rv3096) protein exhibits exo-β-d-galactofuranose hydrolase activity and is capable of hydrolyzing the galactan chain of AG by recurrent cleavage of the terminal β-(1,5) and β-(1,6)-Galf linkages.
32107309	2	41	theme	central	441:447	arg1	portion					449:455	the central portion	437:455	the central portion of the mAGP complex	437:475	Being the central portion of the mAGP complex, arabinogalactan (AG) is believed to be the constituent of the mycobacterial cell envelope that undergoes the least structural changes, but no reports exist supporting this assumption.
32107309	5	42	theme	AG	1236:1237	arg1	remodeling					1208:1217	the remodeling	1204:1217	the remodeling of mycobacterial AG	1204:1237	The characterization of this galactosidase represents a first step toward understanding the remodeling of mycobacterial AG.
32107309	4	43	theme	terminal	1072:1079	arg1	1,5					1084:1086	1,5	1084:1086	1,5	1084:1086	In particular, we found that the mycobacterial GlfH1 (Rv3096) protein exhibits exo-β-d-galactofuranose hydrolase activity and is capable of hydrolyzing the galactan chain of AG by recurrent cleavage of the terminal β-(1,5) and β-(1,6)-Galf linkages.
32107309	4	43	theme	terminal	1072:1079	arg1	β-					1081:1082	the terminal β-	1068:1082	the terminal β-(1,5)	1068:1087	In particular, we found that the mycobacterial GlfH1 (Rv3096) protein exhibits exo-β-d-galactofuranose hydrolase activity and is capable of hydrolyzing the galactan chain of AG by recurrent cleavage of the terminal β-(1,5) and β-(1,6)-Galf linkages.
32107309	1	44	theme	mycolylarabinogalactan-peptidoglycan	163:198	arg1	biogenesis					207:216	mycolylarabinogalactan-peptidoglycan (mAGP) biogenesis	163:216	mycolylarabinogalactan-peptidoglycan (mAGP) biogenesis	163:216	Despite impressive progress made over the past 20 years in our understanding of mycolylarabinogalactan-peptidoglycan (mAGP) biogenesis, the mechanisms by which the tubercle bacillus Mycobacterium tuberculosis adapts its cell wall structure and composition to various environmental conditions, especially during infection, remain poorly understood.
32560213	0	0	theme	Renewable	95:103	arg1	Substrates					105:114	Renewable Substrates	95:114	Renewable Substrates	95:114	Biotechnological Strategies for Chitosan Production by Mucoralean Strains and Dimorphism Using Renewable Substrates.
32560213	1	1	theme	UCP	315:317	arg1	1262					319:322	Mucor subtilissimus UCP 1262	295:322	Mucor subtilissimus UCP 1262	295:322	We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by Mucor subtilissimus UCP 1262 and Lichtheimia hyalospora UCP 1266.
32560213	6	2	from	dimorphism	1101:1110	arg1	CWW					1137:1139	8% CWW	1134:1139	8% CWW	1134:1139	M. subtilissimus (UCP 1262) showed dimorphism in assay 4-6% CSL and 8% CWW and central point.
32560213	6	2	from	dimorphism	1101:1110	arg1	CSL					1126:1128	assay 4-6% CSL	1115:1128	assay 4-6% CSL	1115:1128	M. subtilissimus (UCP 1262) showed dimorphism in assay 4-6% CSL and 8% CWW and central point.
32560213	5	3	theme	chitosan	973:980	arg1	%					953:953	80.29%	948:953	80.29%	948:953	The FTIR bands confirmed the deacetylation degree of 80.29% and 83.61% of the chitosan produced by M. subtilissimus (UCP 1262) and L. hyalospora (UCP 1266), respectively.
32560213	5	3	theme	chitosan	973:980	arg1	%					964:964	83.61%	959:964	83.61% of the chitosan produced by M. subtilissimus (UCP 1262) and L. hyalospora (UCP 1266)	959:1049	The FTIR bands confirmed the deacetylation degree of 80.29% and 83.61% of the chitosan produced by M. subtilissimus (UCP 1262) and L. hyalospora (UCP 1266), respectively.
32560213	5	3	theme	chitosan	973:980	arg1	chitosan					973:980	the chitosan	969:980	the chitosan produced by M. subtilissimus (UCP 1262) and L. hyalospora (UCP 1266)	969:1049	The FTIR bands confirmed the deacetylation degree of 80.29% and 83.61% of the chitosan produced by M. subtilissimus (UCP 1262) and L. hyalospora (UCP 1266), respectively.
32560213	2	4	theme	g/L	439:441	arg1	yields					381:386	The highest biomass yields	361:386	The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266)	361:466	The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 22, and also favored higher chitosan production (32.471 mg/g) for M. subtilissimus.
32560213	5	5	theme	deacetylation	924:936	arg1	degree					938:943	the deacetylation degree	920:943	the deacetylation degree of 80.29% and 83.61% of the chitosan produced by M. subtilissimus (UCP 1262) and L. hyalospora (UCP 1266), respectively	920:1063	The FTIR bands confirmed the deacetylation degree of 80.29% and 83.61% of the chitosan produced by M. subtilissimus (UCP 1262) and L. hyalospora (UCP 1266), respectively.
32560213	6	6	from	point	1153:1157	arg1	CWW					1137:1139	8% CWW	1134:1139	8% CWW	1134:1139	M. subtilissimus (UCP 1262) showed dimorphism in assay 4-6% CSL and 8% CWW and central point.
32560213	6	6	from	point	1153:1157	arg1	CSL					1126:1128	assay 4-6% CSL	1115:1128	assay 4-6% CSL	1115:1128	M. subtilissimus (UCP 1262) showed dimorphism in assay 4-6% CSL and 8% CWW and central point.
32560213	8	7	theme	experimental	1351:1362	arg1	data					1364:1367	The experimental data	1347:1367	The experimental data	1347:1367	The experimental data suggest that the use of CSL and CWW is a promising association to chitosan production.
32560213	7	8	dep	%	1329:1329	arg1	CSL					1331:1333	CSL	1331:1333	8.82% CSL	1325:1333	L. hyalospora (UCP 1266) was optimized using a central composite rotational design, and the highest yield of chitosan (63.18 mg/g) was obtained in medium containing 8.82% CSL and 7% CWW.
32560213	7	9	dep	%	1340:1340	arg1	CWW					1342:1344	CWW	1342:1344	7% CWW	1339:1344	L. hyalospora (UCP 1266) was optimized using a central composite rotational design, and the highest yield of chitosan (63.18 mg/g) was obtained in medium containing 8.82% CSL and 7% CWW.
32560213	3	10	theme	UCP	678:680	arg1	hyalospora					666:675	L. hyalospora	663:675	L. hyalospora (UCP 1266)	663:686	The highest chitosan production (44.91 mg/g) by L. hyalospora (UCP 1266) was obtained at the central point (4% of CWW and 6% of CSL).
32560213	3	10	theme	UCP	678:680	arg1	1266					682:685	UCP 1266	678:685	UCP 1266	678:685	The highest chitosan production (44.91 mg/g) by L. hyalospora (UCP 1266) was obtained at the central point (4% of CWW and 6% of CSL).
32560213	1	11	theme	Lichtheimia	328:338	arg1	1266					355:358	Lichtheimia hyalospora UCP 1266	328:358	Lichtheimia hyalospora UCP 1266	328:358	We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by Mucor subtilissimus UCP 1262 and Lichtheimia hyalospora UCP 1266.
32560213	7	12	theme	L.	1160:1161	arg1	hyalospora					1163:1172	L. hyalospora	1160:1172	L. hyalospora (UCP 1266)	1160:1183	L. hyalospora (UCP 1266) was optimized using a central composite rotational design, and the highest yield of chitosan (63.18 mg/g) was obtained in medium containing 8.82% CSL and 7% CWW.
32560213	7	12	theme	L.	1160:1161	arg1	1266					1179:1182	UCP 1266	1175:1182	UCP 1266	1175:1182	L. hyalospora (UCP 1266) was optimized using a central composite rotational design, and the highest yield of chitosan (63.18 mg/g) was obtained in medium containing 8.82% CSL and 7% CWW.
32560213	7	13	theme	highest	1252:1258	arg1	yield					1260:1264	the highest yield	1248:1264	the highest yield of chitosan (63.18 mg/g)	1248:1289	L. hyalospora (UCP 1266) was optimized using a central composite rotational design, and the highest yield of chitosan (63.18 mg/g) was obtained in medium containing 8.82% CSL and 7% CWW.
32560213	7	14	theme	UCP	1175:1177	arg1	hyalospora					1163:1172	L. hyalospora	1160:1172	L. hyalospora (UCP 1266)	1160:1183	L. hyalospora (UCP 1266) was optimized using a central composite rotational design, and the highest yield of chitosan (63.18 mg/g) was obtained in medium containing 8.82% CSL and 7% CWW.
32560213	7	14	theme	UCP	1175:1177	arg1	1266					1179:1182	UCP 1266	1175:1182	UCP 1266	1175:1182	L. hyalospora (UCP 1266) was optimized using a central composite rotational design, and the highest yield of chitosan (63.18 mg/g) was obtained in medium containing 8.82% CSL and 7% CWW.
32560213	1	15	theme	hyalospora	340:349	arg1	1266					355:358	Lichtheimia hyalospora UCP 1266	328:358	Lichtheimia hyalospora UCP 1266	328:358	We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by Mucor subtilissimus UCP 1262 and Lichtheimia hyalospora UCP 1266.
32560213	2	16	theme	biomass	373:379	arg1	yields					381:386	The highest biomass yields	361:386	The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266)	361:466	The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 22, and also favored higher chitosan production (32.471 mg/g) for M. subtilissimus.
32560213	2	17	theme	UCP	458:460	arg1	1266					462:465	L. hyalospora UCP 1266	444:465	L. hyalospora UCP 1266	444:465	The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 22, and also favored higher chitosan production (32.471 mg/g) for M. subtilissimus.
32560213	2	17	theme	UCP	458:460	arg1	g/L					439:441	6.345 g/L	433:441	6.345 g/L (L. hyalospora UCP 1266)	433:466	The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 22, and also favored higher chitosan production (32.471 mg/g) for M. subtilissimus.
32560213	1	18	theme	UCP	351:353	arg1	1266					355:358	Lichtheimia hyalospora UCP 1266	328:358	Lichtheimia hyalospora UCP 1266	328:358	We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by Mucor subtilissimus UCP 1262 and Lichtheimia hyalospora UCP 1266.
32560213	2	19	theme	factorial	514:522	arg1	assay					485:489	assay 2	485:491	assay 2 (6% CSL and 4% CWW)	485:511	The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 22, and also favored higher chitosan production (32.471 mg/g) for M. subtilissimus.
32560213	2	19	theme	factorial	514:522	arg1	design					524:529	factorial design 22	514:532	factorial design 22	514:532	The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 22, and also favored higher chitosan production (32.471 mg/g) for M. subtilissimus.
32560213	3	20	dep	point	716:720	arg1	CSL					743:745	CSL	743:745	CSL	743:745	The highest chitosan production (44.91 mg/g) by L. hyalospora (UCP 1266) was obtained at the central point (4% of CWW and 6% of CSL).
32560213	3	20	dep	point	716:720	arg1	CWW					729:731	CWW	729:731	CWW	729:731	The highest chitosan production (44.91 mg/g) by L. hyalospora (UCP 1266) was obtained at the central point (4% of CWW and 6% of CSL).
32560213	3	20	dep	point	716:720	arg1	%					738:738	6%	737:738	6% of CSL	737:745	The highest chitosan production (44.91 mg/g) by L. hyalospora (UCP 1266) was obtained at the central point (4% of CWW and 6% of CSL).
32560213	3	20	dep	point	716:720	arg1	%					724:724	4%	723:724	4% of CWW	723:731	The highest chitosan production (44.91 mg/g) by L. hyalospora (UCP 1266) was obtained at the central point (4% of CWW and 6% of CSL).
32560213	2	21	theme	subtilissimus	405:417	arg1	1262					423:426	M. subtilissimus UCP 1262	402:426	M. subtilissimus UCP 1262	402:426	The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 22, and also favored higher chitosan production (32.471 mg/g) for M. subtilissimus.
32560213	2	21	theme	subtilissimus	405:417	arg1	g/L					397:399	4.832 g/L	391:399	4.832 g/L (M. subtilissimus UCP 1262)	391:427	The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 22, and also favored higher chitosan production (32.471 mg/g) for M. subtilissimus.
32560213	2	22	theme	highest	365:371	arg1	yields					381:386	The highest biomass yields	361:386	The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266)	361:466	The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 22, and also favored higher chitosan production (32.471 mg/g) for M. subtilissimus.
32560213	2	23	theme	UCP	419:421	arg1	1262					423:426	M. subtilissimus UCP 1262	402:426	M. subtilissimus UCP 1262	402:426	The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 22, and also favored higher chitosan production (32.471 mg/g) for M. subtilissimus.
32560213	2	23	theme	UCP	419:421	arg1	g/L					397:399	4.832 g/L	391:399	4.832 g/L (M. subtilissimus UCP 1262)	391:427	The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 22, and also favored higher chitosan production (32.471 mg/g) for M. subtilissimus.
32560213	3	24	theme	highest	619:625	arg1	production					636:645	The highest chitosan production	615:645	The highest chitosan production (44.91 mg/g) by L. hyalospora (UCP 1266)	615:686	The highest chitosan production (44.91 mg/g) by L. hyalospora (UCP 1266) was obtained at the central point (4% of CWW and 6% of CSL).
32560213	3	24	theme	highest	619:625	arg1	mg/g					654:657	44.91 mg/g	648:657	44.91 mg/g	648:657	The highest chitosan production (44.91 mg/g) by L. hyalospora (UCP 1266) was obtained at the central point (4% of CWW and 6% of CSL).
32560213	2	25	theme	chitosan	559:566	arg1	mg/g					587:590	32.471 mg/g	580:590	32.471 mg/g	580:590	The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 22, and also favored higher chitosan production (32.471 mg/g) for M. subtilissimus.
32560213	2	25	theme	chitosan	559:566	arg1	production					568:577	higher chitosan production	552:577	higher chitosan production (32.471 mg/g)	552:591	The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 22, and also favored higher chitosan production (32.471 mg/g) for M. subtilissimus.
32560213	7	26	contain	containing	1314:1323	arg2	%					1329:1329	8.82%	1325:1329	8.82% CSL	1325:1333	L. hyalospora (UCP 1266) was optimized using a central composite rotational design, and the highest yield of chitosan (63.18 mg/g) was obtained in medium containing 8.82% CSL and 7% CWW.
32560213	7	26	contain	containing	1314:1323	arg2	%					1340:1340	7%	1339:1340	7% CWW	1339:1344	L. hyalospora (UCP 1266) was optimized using a central composite rotational design, and the highest yield of chitosan (63.18 mg/g) was obtained in medium containing 8.82% CSL and 7% CWW.
32560213	7	26	contain	containing	1314:1323	arg1	medium					1307:1312	medium	1307:1312	medium containing 8.82% CSL and 7% CWW	1307:1344	L. hyalospora (UCP 1266) was optimized using a central composite rotational design, and the highest yield of chitosan (63.18 mg/g) was obtained in medium containing 8.82% CSL and 7% CWW.
32560213	3	27	theme	CWW	729:731	arg1	CSL					743:745	CSL	743:745	CSL	743:745	The highest chitosan production (44.91 mg/g) by L. hyalospora (UCP 1266) was obtained at the central point (4% of CWW and 6% of CSL).
32560213	3	27	theme	CWW	729:731	arg1	CWW					729:731	CWW	729:731	CWW	729:731	The highest chitosan production (44.91 mg/g) by L. hyalospora (UCP 1266) was obtained at the central point (4% of CWW and 6% of CSL).
32560213	3	27	theme	CWW	729:731	arg1	%					738:738	6%	737:738	6% of CSL	737:745	The highest chitosan production (44.91 mg/g) by L. hyalospora (UCP 1266) was obtained at the central point (4% of CWW and 6% of CSL).
32560213	3	27	theme	CWW	729:731	arg1	%					724:724	4%	723:724	4% of CWW	723:731	The highest chitosan production (44.91 mg/g) by L. hyalospora (UCP 1266) was obtained at the central point (4% of CWW and 6% of CSL).
32560213	1	28	theme	cassava	178:184	arg1	CWW					199:201	CWW	199:201	CWW	199:201	We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by Mucor subtilissimus UCP 1262 and Lichtheimia hyalospora UCP 1266.
32560213	1	28	theme	cassava	178:184	arg1	water					192:196	cassava waste water	178:196	cassava waste water (CWW)	178:202	We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by Mucor subtilissimus UCP 1262 and Lichtheimia hyalospora UCP 1266.
32560213	0	29	theme	Biotechnological	0:15	arg1	Strategies					17:26	Biotechnological Strategies	0:26	Biotechnological Strategies for Chitosan Production by Mucoralean Strains and Dimorphism	0:87	Biotechnological Strategies for Chitosan Production by Mucoralean Strains and Dimorphism Using Renewable Substrates.
32560213	3	30	theme	chitosan	627:634	arg1	production					636:645	The highest chitosan production	615:645	The highest chitosan production (44.91 mg/g) by L. hyalospora (UCP 1266)	615:686	The highest chitosan production (44.91 mg/g) by L. hyalospora (UCP 1266) was obtained at the central point (4% of CWW and 6% of CSL).
32560213	3	30	theme	chitosan	627:634	arg1	mg/g					654:657	44.91 mg/g	648:657	44.91 mg/g	648:657	The highest chitosan production (44.91 mg/g) by L. hyalospora (UCP 1266) was obtained at the central point (4% of CWW and 6% of CSL).
32560213	2	31	theme	higher	552:557	arg1	mg/g					587:590	32.471 mg/g	580:590	32.471 mg/g	580:590	The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 22, and also favored higher chitosan production (32.471 mg/g) for M. subtilissimus.
32560213	2	31	theme	higher	552:557	arg1	production					568:577	higher chitosan production	552:577	higher chitosan production (32.471 mg/g)	552:591	The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 22, and also favored higher chitosan production (32.471 mg/g) for M. subtilissimus.
32560213	4	32	theme	lower	812:816	arg1	concentration					818:830	lower concentration	812:830	lower concentration of CWW	812:837	The statistical analysis, the higher concentration of CSL, and lower concentration of CWW significantly contributed to the growth of the strains.
32560213	3	33	theme	CSL	743:745	arg1	CSL					743:745	CSL	743:745	CSL	743:745	The highest chitosan production (44.91 mg/g) by L. hyalospora (UCP 1266) was obtained at the central point (4% of CWW and 6% of CSL).
32560213	3	33	theme	CSL	743:745	arg1	CWW					729:731	CWW	729:731	CWW	729:731	The highest chitosan production (44.91 mg/g) by L. hyalospora (UCP 1266) was obtained at the central point (4% of CWW and 6% of CSL).
32560213	3	33	theme	CSL	743:745	arg1	%					738:738	6%	737:738	6% of CSL	737:745	The highest chitosan production (44.91 mg/g) by L. hyalospora (UCP 1266) was obtained at the central point (4% of CWW and 6% of CSL).
32560213	3	33	theme	CSL	743:745	arg1	%					724:724	4%	723:724	4% of CWW	723:731	The highest chitosan production (44.91 mg/g) by L. hyalospora (UCP 1266) was obtained at the central point (4% of CWW and 6% of CSL).
32560213	1	34	theme	waste	186:190	arg1	CWW					199:201	CWW	199:201	CWW	199:201	We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by Mucor subtilissimus UCP 1262 and Lichtheimia hyalospora UCP 1266.
32560213	1	34	theme	waste	186:190	arg1	water					192:196	cassava waste water	178:196	cassava waste water (CWW)	178:202	We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by Mucor subtilissimus UCP 1262 and Lichtheimia hyalospora UCP 1266.
32560213	0	35	theme	Chitosan	32:39	arg1	Production					41:50	Chitosan Production	32:50	Chitosan Production by Mucoralean Strains	32:72	Biotechnological Strategies for Chitosan Production by Mucoralean Strains and Dimorphism Using Renewable Substrates.
32560213	5	36	theme	%	964:964	arg1	degree					938:943	the deacetylation degree	920:943	the deacetylation degree of 80.29% and 83.61% of the chitosan produced by M. subtilissimus (UCP 1262) and L. hyalospora (UCP 1266), respectively	920:1063	The FTIR bands confirmed the deacetylation degree of 80.29% and 83.61% of the chitosan produced by M. subtilissimus (UCP 1262) and L. hyalospora (UCP 1266), respectively.
32560213	4	37	theme	statistical	753:763	arg1	analysis					765:772	The statistical analysis	749:772	The statistical analysis	749:772	The statistical analysis, the higher concentration of CSL, and lower concentration of CWW significantly contributed to the growth of the strains.
32560213	1	38	from	influence	137:145	arg1	production					257:266	production	257:266	production	257:266	We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by Mucor subtilissimus UCP 1262 and Lichtheimia hyalospora UCP 1266.
32560213	1	38	from	influence	137:145	arg1	morphology					242:251	morphology	242:251	morphology	242:251	We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by Mucor subtilissimus UCP 1262 and Lichtheimia hyalospora UCP 1266.
32560213	6	39	theme	assay	1115:1119	arg1	CSL					1126:1128	assay 4-6% CSL	1115:1128	assay 4-6% CSL	1115:1128	M. subtilissimus (UCP 1262) showed dimorphism in assay 4-6% CSL and 8% CWW and central point.
32560213	1	40	theme	water	192:196	arg1	influence					137:145	the influence	133:145	the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan	133:290	We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by Mucor subtilissimus UCP 1262 and Lichtheimia hyalospora UCP 1266.
32560213	8	41	theme	CSL	1393:1395	arg1	association					1420:1430	a promising association	1408:1430	a promising association to chitosan production	1408:1453	The experimental data suggest that the use of CSL and CWW is a promising association to chitosan production.
32560213	8	41	theme	CSL	1393:1395	arg1	use					1386:1388	the use	1382:1388	the use of CSL and CWW	1382:1403	The experimental data suggest that the use of CSL and CWW is a promising association to chitosan production.
32560213	4	42	theme	CSL	803:805	arg1	concentration					818:830	lower concentration	812:830	lower concentration of CWW	812:837	The statistical analysis, the higher concentration of CSL, and lower concentration of CWW significantly contributed to the growth of the strains.
32560213	4	42	theme	CSL	803:805	arg1	analysis					765:772	The statistical analysis	749:772	The statistical analysis	749:772	The statistical analysis, the higher concentration of CSL, and lower concentration of CWW significantly contributed to the growth of the strains.
32560213	4	42	theme	CSL	803:805	arg1	concentration					786:798	the higher concentration	775:798	the higher concentration of CSL	775:805	The statistical analysis, the higher concentration of CSL, and lower concentration of CWW significantly contributed to the growth of the strains.
32560213	1	43	theme	biomass	271:277	arg1	production					257:266	production	257:266	production	257:266	We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by Mucor subtilissimus UCP 1262 and Lichtheimia hyalospora UCP 1266.
32560213	1	43	theme	biomass	271:277	arg1	morphology					242:251	morphology	242:251	morphology	242:251	We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by Mucor subtilissimus UCP 1262 and Lichtheimia hyalospora UCP 1266.
32560213	4	44	theme	CWW	835:837	arg1	concentration					818:830	lower concentration	812:830	lower concentration of CWW	812:837	The statistical analysis, the higher concentration of CSL, and lower concentration of CWW significantly contributed to the growth of the strains.
32560213	4	44	theme	CWW	835:837	arg1	analysis					765:772	The statistical analysis	749:772	The statistical analysis	749:772	The statistical analysis, the higher concentration of CSL, and lower concentration of CWW significantly contributed to the growth of the strains.
32560213	4	44	theme	CWW	835:837	arg1	concentration					786:798	the higher concentration	775:798	the higher concentration of CSL	775:805	The statistical analysis, the higher concentration of CSL, and lower concentration of CWW significantly contributed to the growth of the strains.
32560213	6	45	theme	%	1135:1135	arg1	CWW					1137:1139	8% CWW	1134:1139	8% CWW	1134:1139	M. subtilissimus (UCP 1262) showed dimorphism in assay 4-6% CSL and 8% CWW and central point.
32560213	4	46	theme	higher	779:784	arg1	concentration					786:798	the higher concentration	775:798	the higher concentration of CSL	775:805	The statistical analysis, the higher concentration of CSL, and lower concentration of CWW significantly contributed to the growth of the strains.
32560213	7	47	theme	rotational	1225:1234	arg1	design					1236:1241	a central composite rotational design	1205:1241	a central composite rotational design	1205:1241	L. hyalospora (UCP 1266) was optimized using a central composite rotational design, and the highest yield of chitosan (63.18 mg/g) was obtained in medium containing 8.82% CSL and 7% CWW.
32560213	2	48	theme	M.	402:403	arg1	1262					423:426	M. subtilissimus UCP 1262	402:426	M. subtilissimus UCP 1262	402:426	The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 22, and also favored higher chitosan production (32.471 mg/g) for M. subtilissimus.
32560213	2	48	theme	M.	402:403	arg1	g/L					397:399	4.832 g/L	391:399	4.832 g/L (M. subtilissimus UCP 1262)	391:427	The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 22, and also favored higher chitosan production (32.471 mg/g) for M. subtilissimus.
32560213	2	49	dep	assay	485:489	arg1	%					506:506	6% CSL and 4% CWW	494:510	%	506:506	The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 22, and also favored higher chitosan production (32.471 mg/g) for M. subtilissimus.
32560213	2	49	dep	assay	485:489	arg1	CSL					497:499	6% CSL and 4% CWW	494:510	CSL	497:499	The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 22, and also favored higher chitosan production (32.471 mg/g) for M. subtilissimus.
32560213	6	50	theme	8	1134:1134	arg1	%					1135:1135	%	1135:1135	%	1135:1135	M. subtilissimus (UCP 1262) showed dimorphism in assay 4-6% CSL and 8% CWW and central point.
32560213	1	51	theme	chitosan	283:290	arg1	production					257:266	production	257:266	production	257:266	We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by Mucor subtilissimus UCP 1262 and Lichtheimia hyalospora UCP 1266.
32560213	1	51	theme	chitosan	283:290	arg1	morphology					242:251	morphology	242:251	morphology	242:251	We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by Mucor subtilissimus UCP 1262 and Lichtheimia hyalospora UCP 1266.
32560213	6	52	theme	%	1124:1124	arg1	CSL					1126:1128	assay 4-6% CSL	1115:1128	assay 4-6% CSL	1115:1128	M. subtilissimus (UCP 1262) showed dimorphism in assay 4-6% CSL and 8% CWW and central point.
32560213	5	53	theme	FTIR	899:902	arg1	bands					904:908	The FTIR bands	895:908	The FTIR bands	895:908	The FTIR bands confirmed the deacetylation degree of 80.29% and 83.61% of the chitosan produced by M. subtilissimus (UCP 1262) and L. hyalospora (UCP 1266), respectively.
32560213	0	54	theme	Mucoralean	55:64	arg1	Strains					66:72	Mucoralean Strains	55:72	Mucoralean Strains	55:72	Biotechnological Strategies for Chitosan Production by Mucoralean Strains and Dimorphism Using Renewable Substrates.
32560213	5	55	theme	%	953:953	arg1	degree					938:943	the deacetylation degree	920:943	the deacetylation degree of 80.29% and 83.61% of the chitosan produced by M. subtilissimus (UCP 1262) and L. hyalospora (UCP 1266), respectively	920:1063	The FTIR bands confirmed the deacetylation degree of 80.29% and 83.61% of the chitosan produced by M. subtilissimus (UCP 1262) and L. hyalospora (UCP 1266), respectively.
32560213	2	56	theme	%	495:495	arg1	CSL					497:499	6% CSL and 4% CWW	494:510	CSL	497:499	The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 22, and also favored higher chitosan production (32.471 mg/g) for M. subtilissimus.
32560213	7	57	theme	central	1207:1213	arg1	design					1236:1241	a central composite rotational design	1205:1241	a central composite rotational design	1205:1241	L. hyalospora (UCP 1266) was optimized using a central composite rotational design, and the highest yield of chitosan (63.18 mg/g) was obtained in medium containing 8.82% CSL and 7% CWW.
32560213	7	58	theme	composite	1215:1223	arg1	design					1236:1241	a central composite rotational design	1205:1241	a central composite rotational design	1205:1241	L. hyalospora (UCP 1266) was optimized using a central composite rotational design, and the highest yield of chitosan (63.18 mg/g) was obtained in medium containing 8.82% CSL and 7% CWW.
32560213	8	59	theme	promising	1410:1418	arg1	use					1386:1388	the use	1382:1388	the use of CSL and CWW	1382:1403	The experimental data suggest that the use of CSL and CWW is a promising association to chitosan production.
32560213	8	59	theme	promising	1410:1418	arg1	association					1420:1430	a promising association	1408:1430	a promising association to chitosan production	1408:1453	The experimental data suggest that the use of CSL and CWW is a promising association to chitosan production.
32560213	8	60	theme	chitosan	1435:1442	arg1	production					1444:1453	chitosan production	1435:1453	chitosan production	1435:1453	The experimental data suggest that the use of CSL and CWW is a promising association to chitosan production.
32560213	1	61	theme	Mucor	295:299	arg1	1262					319:322	Mucor subtilissimus UCP 1262	295:322	Mucor subtilissimus UCP 1262	295:322	We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by Mucor subtilissimus UCP 1262 and Lichtheimia hyalospora UCP 1266.
32560213	1	62	dep	morphology	242:251	arg1	the					238:240	the	238:240	the	238:240	We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by Mucor subtilissimus UCP 1262 and Lichtheimia hyalospora UCP 1266.
32560213	3	63	theme	central	708:714	arg1	point					716:720	the central point	704:720	the central point (4% of CWW and 6% of CSL)	704:746	The highest chitosan production (44.91 mg/g) by L. hyalospora (UCP 1266) was obtained at the central point (4% of CWW and 6% of CSL).
32560213	1	64	theme	corn	150:153	arg1	liquor					161:166	corn steep liquor	150:166	corn steep liquor (CSL)	150:172	We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by Mucor subtilissimus UCP 1262 and Lichtheimia hyalospora UCP 1266.
32560213	1	64	theme	corn	150:153	arg1	CSL					169:171	CSL	169:171	CSL	169:171	We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by Mucor subtilissimus UCP 1262 and Lichtheimia hyalospora UCP 1266.
32560213	4	65	theme	strains	886:892	arg1	growth					872:877	the growth	868:877	the growth of the strains	868:892	The statistical analysis, the higher concentration of CSL, and lower concentration of CWW significantly contributed to the growth of the strains.
32560213	1	66	theme	carbon	207:212	arg1	sources					227:233	carbon and nitrogen sources	207:233	sources	227:233	We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by Mucor subtilissimus UCP 1262 and Lichtheimia hyalospora UCP 1266.
32560213	2	67	theme	hyalospora	447:456	arg1	1266					462:465	L. hyalospora UCP 1266	444:465	L. hyalospora UCP 1266	444:465	The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 22, and also favored higher chitosan production (32.471 mg/g) for M. subtilissimus.
32560213	2	67	theme	hyalospora	447:456	arg1	g/L					439:441	6.345 g/L	433:441	6.345 g/L (L. hyalospora UCP 1266)	433:466	The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 22, and also favored higher chitosan production (32.471 mg/g) for M. subtilissimus.
32560213	6	68	theme	central	1145:1151	arg1	point					1153:1157	central point	1145:1157	central point	1145:1157	M. subtilissimus (UCP 1262) showed dimorphism in assay 4-6% CSL and 8% CWW and central point.
32560213	2	69	theme	g/L	397:399	arg1	yields					381:386	The highest biomass yields	361:386	The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266)	361:466	The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 22, and also favored higher chitosan production (32.471 mg/g) for M. subtilissimus.
32560213	1	70	theme	steep	155:159	arg1	liquor					161:166	corn steep liquor	150:166	corn steep liquor (CSL)	150:172	We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by Mucor subtilissimus UCP 1262 and Lichtheimia hyalospora UCP 1266.
32560213	1	70	theme	steep	155:159	arg1	CSL					169:171	CSL	169:171	CSL	169:171	We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by Mucor subtilissimus UCP 1262 and Lichtheimia hyalospora UCP 1266.
32560213	5	71	theme	UCP	1041:1043	arg1	hyalospora					1029:1038	L. hyalospora	1026:1038	L. hyalospora (UCP 1266)	1026:1049	The FTIR bands confirmed the deacetylation degree of 80.29% and 83.61% of the chitosan produced by M. subtilissimus (UCP 1262) and L. hyalospora (UCP 1266), respectively.
32560213	5	71	theme	UCP	1041:1043	arg1	1266					1045:1048	UCP 1266	1041:1048	UCP 1266	1041:1048	The FTIR bands confirmed the deacetylation degree of 80.29% and 83.61% of the chitosan produced by M. subtilissimus (UCP 1262) and L. hyalospora (UCP 1266), respectively.
32560213	7	72	theme	chitosan	1269:1276	arg1	yield					1260:1264	the highest yield	1248:1264	the highest yield of chitosan (63.18 mg/g)	1248:1289	L. hyalospora (UCP 1266) was optimized using a central composite rotational design, and the highest yield of chitosan (63.18 mg/g) was obtained in medium containing 8.82% CSL and 7% CWW.
32560213	2	73	theme	L.	444:445	arg1	1266					462:465	L. hyalospora UCP 1266	444:465	L. hyalospora UCP 1266	444:465	The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 22, and also favored higher chitosan production (32.471 mg/g) for M. subtilissimus.
32560213	2	73	theme	L.	444:445	arg1	g/L					439:441	6.345 g/L	433:441	6.345 g/L (L. hyalospora UCP 1266)	433:466	The highest biomass yields of 4.832 g/L (M. subtilissimus UCP 1262) and 6.345 g/L (L. hyalospora UCP 1266) were produced in assay 2 (6% CSL and 4% CWW), factorial design 22, and also favored higher chitosan production (32.471 mg/g) for M. subtilissimus.
32560213	1	74	theme	subtilissimus	301:313	arg1	1262					319:322	Mucor subtilissimus UCP 1262	295:322	Mucor subtilissimus UCP 1262	295:322	We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by Mucor subtilissimus UCP 1262 and Lichtheimia hyalospora UCP 1266.
32560213	1	75	theme	liquor	161:166	arg1	influence					137:145	the influence	133:145	the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan	133:290	We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by Mucor subtilissimus UCP 1262 and Lichtheimia hyalospora UCP 1266.
32560213	8	76	theme	CWW	1401:1403	arg1	association					1420:1430	a promising association	1408:1430	a promising association to chitosan production	1408:1453	The experimental data suggest that the use of CSL and CWW is a promising association to chitosan production.
32560213	8	76	theme	CWW	1401:1403	arg1	use					1386:1388	the use	1382:1388	the use of CSL and CWW	1382:1403	The experimental data suggest that the use of CSL and CWW is a promising association to chitosan production.
32560213	1	77	theme	nitrogen	218:225	arg1	sources					227:233	carbon and nitrogen sources	207:233	sources	227:233	We investigated the influence of corn steep liquor (CSL) and cassava waste water (CWW) as carbon and nitrogen sources on the morphology and production of biomass and chitosan by Mucor subtilissimus UCP 1262 and Lichtheimia hyalospora UCP 1266.
33124483	0	0	theme	cell	95:98	arg1	line					100:103	human lung cancer cell line A549	77:108	human lung cancer cell line A549	77:108	Antiproliferative effects of boswellic acid-loaded chitosan nanoparticles on human lung cancer cell line A549.
33124483	1	1	theme	acids-loaded	147:158	arg1	nanoparticles					169:181	boswellic acids-loaded chitosan nanoparticles	137:181	boswellic acids-loaded chitosan nanoparticles	137:181	Aim: In the present study boswellic acids-loaded chitosan nanoparticles were synthesized using ionic gelation technique.
33124483	6	2	theme	acids-loaded	924:935	arg1	nanoparticles					946:958	the boswellic acids-loaded chitosan nanoparticles	910:958	the boswellic acids-loaded chitosan nanoparticles	910:958	The observed results justified the boswellic acids-loaded chitosan nanoparticles were effective due to greater cellular uptake, sustained intercellular drug retention and enhanced antiproliferative effect by inducing apoptosis.
33124483	3	3	dep	results	367:373	arg1	observed					408:415	observed	408:415	were observed spherical in shape with an average size of 67.5-187.2 nm	403:472	Methodology & results: The designed nanoparticles were observed spherical in shape with an average size of 67.5-187.2 nm and have also shown an excellent entrapment efficiency (80.06 ± 0.48).
33124483	3	3	dep	results	367:373	arg1	shown					488:492	shown	488:492	have also shown an excellent entrapment efficiency (80.06 ± 0.48)	478:542	Methodology & results: The designed nanoparticles were observed spherical in shape with an average size of 67.5-187.2 nm and have also shown an excellent entrapment efficiency (80.06 ± 0.48).
33124483	0	4	theme	cancer	88:93	arg1	line					100:103	human lung cancer cell line A549	77:108	human lung cancer cell line A549	77:108	Antiproliferative effects of boswellic acid-loaded chitosan nanoparticles on human lung cancer cell line A549.
33124483	0	5	from	effects	18:24	arg1	line					100:103	human lung cancer cell line A549	77:108	human lung cancer cell line A549	77:108	Antiproliferative effects of boswellic acid-loaded chitosan nanoparticles on human lung cancer cell line A549.
33124483	1	6	theme	chitosan	160:167	arg1	nanoparticles					169:181	boswellic acids-loaded chitosan nanoparticles	137:181	boswellic acids-loaded chitosan nanoparticles	137:181	Aim: In the present study boswellic acids-loaded chitosan nanoparticles were synthesized using ionic gelation technique.
33124483	5	7	theme	arrested	783:790	arg1	%					803:803	arrested 22.75 ± 0.3%	783:803	arrested 22.75 ± 0.3%	783:803	Flow cytometry confirmed that treatment of cells with 40 μg/ml had arrested 22.75 ± 0.3% at SubG0 phase of the cell cycle when compared with untreated A459 cells.
33124483	3	8	theme	designed	380:387	arg1	nanoparticles					389:401	The designed nanoparticles	376:401	The designed nanoparticles	376:401	Methodology & results: The designed nanoparticles were observed spherical in shape with an average size of 67.5-187.2 nm and have also shown an excellent entrapment efficiency (80.06 ± 0.48).
33124483	6	9	theme	chitosan	937:944	arg1	nanoparticles					946:958	the boswellic acids-loaded chitosan nanoparticles	910:958	the boswellic acids-loaded chitosan nanoparticles	910:958	The observed results justified the boswellic acids-loaded chitosan nanoparticles were effective due to greater cellular uptake, sustained intercellular drug retention and enhanced antiproliferative effect by inducing apoptosis.
33124483	5	10	contain	had	779:781	arg2	%					803:803	arrested 22.75 ± 0.3%	783:803	arrested 22.75 ± 0.3%	783:803	Flow cytometry confirmed that treatment of cells with 40 μg/ml had arrested 22.75 ± 0.3% at SubG0 phase of the cell cycle when compared with untreated A459 cells.
33124483	5	10	contain	had	779:781	arg1	treatment					746:754	treatment	746:754	treatment of cells with 40 μg/ml	746:777	Flow cytometry confirmed that treatment of cells with 40 μg/ml had arrested 22.75 ± 0.3% at SubG0 phase of the cell cycle when compared with untreated A459 cells.
33124483	2	11	theme	dependent	301:309	arg1	variables					311:319	dependent variables	301:319	dependent variables using central composite design	301:350	The influence of independent variables were studied and optimized on dependent variables using central composite design.
33124483	3	12	theme	excellent	497:505	arg1	80.06 ± 0.48					530:541	80.06 ± 0.48	530:541	80.06 ± 0.48	530:541	Methodology & results: The designed nanoparticles were observed spherical in shape with an average size of 67.5-187.2 nm and have also shown an excellent entrapment efficiency (80.06 ± 0.48).
33124483	3	12	theme	excellent	497:505	arg1	efficiency					518:527	an excellent entrapment efficiency	494:527	an excellent entrapment efficiency (80.06 ± 0.48)	494:542	Methodology & results: The designed nanoparticles were observed spherical in shape with an average size of 67.5-187.2 nm and have also shown an excellent entrapment efficiency (80.06 ± 0.48).
33124483	6	13	theme	boswellic	914:922	arg1	nanoparticles					946:958	the boswellic acids-loaded chitosan nanoparticles	910:958	the boswellic acids-loaded chitosan nanoparticles	910:958	The observed results justified the boswellic acids-loaded chitosan nanoparticles were effective due to greater cellular uptake, sustained intercellular drug retention and enhanced antiproliferative effect by inducing apoptosis.
33124483	5	14	theme	untreated	857:865	arg1	cells					872:876	untreated A459 cells	857:876	untreated A459 cells	857:876	Flow cytometry confirmed that treatment of cells with 40 μg/ml had arrested 22.75 ± 0.3% at SubG0 phase of the cell cycle when compared with untreated A459 cells.
33124483	6	15	theme	drug	1031:1034	arg1	retention					1036:1044	intercellular drug retention	1017:1044	intercellular drug retention	1017:1044	The observed results justified the boswellic acids-loaded chitosan nanoparticles were effective due to greater cellular uptake, sustained intercellular drug retention and enhanced antiproliferative effect by inducing apoptosis.
33124483	3	16	from	spherical	417:425	arg1	shape					430:434	shape	430:434	shape	430:434	Methodology & results: The designed nanoparticles were observed spherical in shape with an average size of 67.5-187.2 nm and have also shown an excellent entrapment efficiency (80.06 ± 0.48).
33124483	5	17	theme	SubG0	808:812	arg1	phase					814:818	SubG0 phase	808:818	SubG0 phase of the cell cycle	808:836	Flow cytometry confirmed that treatment of cells with 40 μg/ml had arrested 22.75 ± 0.3% at SubG0 phase of the cell cycle when compared with untreated A459 cells.
33124483	5	18	theme	cycle	832:836	arg1	phase					814:818	SubG0 phase	808:818	SubG0 phase of the cell cycle	808:836	Flow cytometry confirmed that treatment of cells with 40 μg/ml had arrested 22.75 ± 0.3% at SubG0 phase of the cell cycle when compared with untreated A459 cells.
33124483	3	19	theme	nm	471:472	arg1	size					452:455	an average size	441:455	an average size of 67.5-187.2 nm	441:472	Methodology & results: The designed nanoparticles were observed spherical in shape with an average size of 67.5-187.2 nm and have also shown an excellent entrapment efficiency (80.06 ± 0.48).
33124483	5	20	theme	cell	827:830	arg1	cycle					832:836	the cell cycle	823:836	the cell cycle	823:836	Flow cytometry confirmed that treatment of cells with 40 μg/ml had arrested 22.75 ± 0.3% at SubG0 phase of the cell cycle when compared with untreated A459 cells.
33124483	3	21	theme	Methodology	353:363	arg1	results					367:373	Methodology & results	353:373	Methodology & results: The designed nanoparticles were observed spherical in shape with an average size of 67.5-187.2 nm and have also shown an excellent entrapment efficiency (80.06 ± 0.48).	353:543	Methodology & results: The designed nanoparticles were observed spherical in shape with an average size of 67.5-187.2 nm and have also shown an excellent entrapment efficiency (80.06 ± 0.48).
33124483	3	22	theme	&	365:365	arg1	results					367:373	Methodology & results	353:373	Methodology & results: The designed nanoparticles were observed spherical in shape with an average size of 67.5-187.2 nm and have also shown an excellent entrapment efficiency (80.06 ± 0.48).	353:543	Methodology & results: The designed nanoparticles were observed spherical in shape with an average size of 67.5-187.2 nm and have also shown an excellent entrapment efficiency (80.06 ± 0.48).
33124483	0	23	theme	Antiproliferative	0:16	arg1	effects					18:24	Antiproliferative effects	0:24	Antiproliferative effects of boswellic acid-loaded chitosan nanoparticles on human lung cancer cell line A549.	0:109	Antiproliferative effects of boswellic acid-loaded chitosan nanoparticles on human lung cancer cell line A549.
33124483	6	24	theme	intercellular	1017:1029	arg1	retention					1036:1044	intercellular drug retention	1017:1044	intercellular drug retention	1017:1044	The observed results justified the boswellic acids-loaded chitosan nanoparticles were effective due to greater cellular uptake, sustained intercellular drug retention and enhanced antiproliferative effect by inducing apoptosis.
33124483	2	25	theme	composite	335:343	arg1	design					345:350	central composite design	327:350	central composite design	327:350	The influence of independent variables were studied and optimized on dependent variables using central composite design.
33124483	4	26	theme	29.59 μM	692:699	arg1	value					673:677	an IC50 value	665:677	an IC50 value of 17.29 and 29.59 μM	665:699	The cytotoxicity assay revealed enhanced cytotoxicity for drug-loaded nanoparticles in contrast to the free drug having an IC50 value of 17.29 and 29.59 μM, respectively.
33124483	0	27	theme	boswellic	29:37	arg1	nanoparticles					60:72	boswellic acid-loaded chitosan nanoparticles	29:72	boswellic acid-loaded chitosan nanoparticles	29:72	Antiproliferative effects of boswellic acid-loaded chitosan nanoparticles on human lung cancer cell line A549.
33124483	4	28	theme	enhanced	577:584	arg1	cytotoxicity					586:597	enhanced cytotoxicity	577:597	enhanced cytotoxicity	577:597	The cytotoxicity assay revealed enhanced cytotoxicity for drug-loaded nanoparticles in contrast to the free drug having an IC50 value of 17.29 and 29.59 μM, respectively.
33124483	2	29	theme	central	327:333	arg1	design					345:350	central composite design	327:350	central composite design	327:350	The influence of independent variables were studied and optimized on dependent variables using central composite design.
33124483	1	30	theme	ionic	206:210	arg1	technique					221:229	ionic gelation technique	206:229	ionic gelation technique	206:229	Aim: In the present study boswellic acids-loaded chitosan nanoparticles were synthesized using ionic gelation technique.
33124483	4	31	theme	drug-loaded	603:613	arg1	nanoparticles					615:627	drug-loaded nanoparticles	603:627	drug-loaded nanoparticles	603:627	The cytotoxicity assay revealed enhanced cytotoxicity for drug-loaded nanoparticles in contrast to the free drug having an IC50 value of 17.29 and 29.59 μM, respectively.
33124483	1	32	theme	gelation	212:219	arg1	technique					221:229	ionic gelation technique	206:229	ionic gelation technique	206:229	Aim: In the present study boswellic acids-loaded chitosan nanoparticles were synthesized using ionic gelation technique.
33124483	0	33	theme	chitosan	51:58	arg1	nanoparticles					60:72	boswellic acid-loaded chitosan nanoparticles	29:72	boswellic acid-loaded chitosan nanoparticles	29:72	Antiproliferative effects of boswellic acid-loaded chitosan nanoparticles on human lung cancer cell line A549.
33124483	5	34	with	treatment	746:754	arg1	40 μg/ml					770:777	40 μg/ml	770:777	40 μg/ml	770:777	Flow cytometry confirmed that treatment of cells with 40 μg/ml had arrested 22.75 ± 0.3% at SubG0 phase of the cell cycle when compared with untreated A459 cells.
33124483	6	35	theme	observed	883:890	arg1	effective					965:973	effective	965:973	effective	965:973	The observed results justified the boswellic acids-loaded chitosan nanoparticles were effective due to greater cellular uptake, sustained intercellular drug retention and enhanced antiproliferative effect by inducing apoptosis.
33124483	6	35	theme	observed	883:890	arg1	results					892:898	The observed results	879:898	The observed results justified the boswellic acids-loaded chitosan nanoparticles	879:958	The observed results justified the boswellic acids-loaded chitosan nanoparticles were effective due to greater cellular uptake, sustained intercellular drug retention and enhanced antiproliferative effect by inducing apoptosis.
33124483	2	36	theme	independent	249:259	arg1	variables					261:269	independent variables	249:269	independent variables	249:269	The influence of independent variables were studied and optimized on dependent variables using central composite design.
33124483	5	37	theme	A459	867:870	arg1	cells					872:876	untreated A459 cells	857:876	untreated A459 cells	857:876	Flow cytometry confirmed that treatment of cells with 40 μg/ml had arrested 22.75 ± 0.3% at SubG0 phase of the cell cycle when compared with untreated A459 cells.
33124483	0	38	theme	acid-loaded	39:49	arg1	nanoparticles					60:72	boswellic acid-loaded chitosan nanoparticles	29:72	boswellic acid-loaded chitosan nanoparticles	29:72	Antiproliferative effects of boswellic acid-loaded chitosan nanoparticles on human lung cancer cell line A549.
33124483	4	39	theme	cytotoxicity	549:560	arg1	assay					562:566	The cytotoxicity assay	545:566	The cytotoxicity assay	545:566	The cytotoxicity assay revealed enhanced cytotoxicity for drug-loaded nanoparticles in contrast to the free drug having an IC50 value of 17.29 and 29.59 μM, respectively.
33124483	3	40	theme	average	444:450	arg1	size					452:455	an average size	441:455	an average size of 67.5-187.2 nm	441:472	Methodology & results: The designed nanoparticles were observed spherical in shape with an average size of 67.5-187.2 nm and have also shown an excellent entrapment efficiency (80.06 ± 0.48).
33124483	5	41	theme	cells	759:763	arg1	treatment					746:754	treatment	746:754	treatment of cells with 40 μg/ml	746:777	Flow cytometry confirmed that treatment of cells with 40 μg/ml had arrested 22.75 ± 0.3% at SubG0 phase of the cell cycle when compared with untreated A459 cells.
33124483	3	42	theme	entrapment	507:516	arg1	80.06 ± 0.48					530:541	80.06 ± 0.48	530:541	80.06 ± 0.48	530:541	Methodology & results: The designed nanoparticles were observed spherical in shape with an average size of 67.5-187.2 nm and have also shown an excellent entrapment efficiency (80.06 ± 0.48).
33124483	3	42	theme	entrapment	507:516	arg1	efficiency					518:527	an excellent entrapment efficiency	494:527	an excellent entrapment efficiency (80.06 ± 0.48)	494:542	Methodology & results: The designed nanoparticles were observed spherical in shape with an average size of 67.5-187.2 nm and have also shown an excellent entrapment efficiency (80.06 ± 0.48).
33124483	0	43	theme	nanoparticles	60:72	arg1	effects					18:24	Antiproliferative effects	0:24	Antiproliferative effects of boswellic acid-loaded chitosan nanoparticles on human lung cancer cell line A549.	0:109	Antiproliferative effects of boswellic acid-loaded chitosan nanoparticles on human lung cancer cell line A549.
33124483	4	44	dep	having	658:663	arg1	contrast					632:639	contrast	632:639	contrast	632:639	The cytotoxicity assay revealed enhanced cytotoxicity for drug-loaded nanoparticles in contrast to the free drug having an IC50 value of 17.29 and 29.59 μM, respectively.
33124483	6	45	theme	cellular	990:997	arg1	uptake					999:1004	greater cellular uptake	982:1004	greater cellular uptake	982:1004	The observed results justified the boswellic acids-loaded chitosan nanoparticles were effective due to greater cellular uptake, sustained intercellular drug retention and enhanced antiproliferative effect by inducing apoptosis.
33124483	5	46	theme	Flow	716:719	arg1	cytometry					721:729	Flow cytometry	716:729	Flow cytometry	716:729	Flow cytometry confirmed that treatment of cells with 40 μg/ml had arrested 22.75 ± 0.3% at SubG0 phase of the cell cycle when compared with untreated A459 cells.
33124483	3	47	with	spherical	417:425	arg1	size					452:455	an average size	441:455	an average size of 67.5-187.2 nm	441:472	Methodology & results: The designed nanoparticles were observed spherical in shape with an average size of 67.5-187.2 nm and have also shown an excellent entrapment efficiency (80.06 ± 0.48).
33124483	4	48	theme	free	648:651	arg1	drug					653:656	the free drug	644:656	the free drug	644:656	The cytotoxicity assay revealed enhanced cytotoxicity for drug-loaded nanoparticles in contrast to the free drug having an IC50 value of 17.29 and 29.59 μM, respectively.
33124483	1	49	theme	present	123:129	arg1	study					131:135	the present study	119:135	the present study	119:135	Aim: In the present study boswellic acids-loaded chitosan nanoparticles were synthesized using ionic gelation technique.
33124483	0	50	theme	lung	83:86	arg1	line					100:103	human lung cancer cell line A549	77:108	human lung cancer cell line A549	77:108	Antiproliferative effects of boswellic acid-loaded chitosan nanoparticles on human lung cancer cell line A549.
33124483	4	51	theme	IC50	668:671	arg1	value					673:677	an IC50 value	665:677	an IC50 value of 17.29 and 29.59 μM	665:699	The cytotoxicity assay revealed enhanced cytotoxicity for drug-loaded nanoparticles in contrast to the free drug having an IC50 value of 17.29 and 29.59 μM, respectively.
33124483	4	52	theme	17.29	682:686	arg1	value					673:677	an IC50 value	665:677	an IC50 value of 17.29 and 29.59 μM	665:699	The cytotoxicity assay revealed enhanced cytotoxicity for drug-loaded nanoparticles in contrast to the free drug having an IC50 value of 17.29 and 29.59 μM, respectively.
33124483	0	53	theme	human	77:81	arg1	line					100:103	human lung cancer cell line A549	77:108	human lung cancer cell line A549	77:108	Antiproliferative effects of boswellic acid-loaded chitosan nanoparticles on human lung cancer cell line A549.
33124483	3	54	from	shape	430:434	arg1	spherical					417:425	spherical	417:425	spherical	417:425	Methodology & results: The designed nanoparticles were observed spherical in shape with an average size of 67.5-187.2 nm and have also shown an excellent entrapment efficiency (80.06 ± 0.48).
33124483	2	55	theme	variables	261:269	arg1	influence					236:244	The influence	232:244	The influence of independent variables	232:269	The influence of independent variables were studied and optimized on dependent variables using central composite design.
33124483	1	56	theme	boswellic	137:145	arg1	nanoparticles					169:181	boswellic acids-loaded chitosan nanoparticles	137:181	boswellic acids-loaded chitosan nanoparticles	137:181	Aim: In the present study boswellic acids-loaded chitosan nanoparticles were synthesized using ionic gelation technique.
33124483	6	57	theme	greater	982:988	arg1	uptake					999:1004	greater cellular uptake	982:1004	greater cellular uptake	982:1004	The observed results justified the boswellic acids-loaded chitosan nanoparticles were effective due to greater cellular uptake, sustained intercellular drug retention and enhanced antiproliferative effect by inducing apoptosis.
33124483	6	58	theme	antiproliferative	1059:1075	arg1	effect					1077:1082	antiproliferative effect	1059:1082	antiproliferative effect	1059:1082	The observed results justified the boswellic acids-loaded chitosan nanoparticles were effective due to greater cellular uptake, sustained intercellular drug retention and enhanced antiproliferative effect by inducing apoptosis.
34707774	7	0	from	apoptosis	1572:1580	arg1	tissues					1598:1604	rat prostate tissues	1585:1604	rat prostate tissues	1585:1604	We found that simultaneous periodontitis and BPH synergistically aggravated prostate histological changes, significantly increased Ki67 proliferation, and reduced apoptosis in rat prostate tissues.
34707774	1	1	theme	prostatic	282:290	arg1	symptom					292:298	the prostatic symptom	278:298	the prostatic symptom	278:298	Epidemiological studies demonstrate that men with periodontitis are also susceptible to benign prostatic hyperplasia (BPH) and that periodontal treatment can improve the prostatic symptom.
34707774	7	2	theme	Ki67	1540:1543	arg1	proliferation					1545:1557	significantly increased Ki67 proliferation	1516:1557	significantly increased Ki67 proliferation	1516:1557	We found that simultaneous periodontitis and BPH synergistically aggravated prostate histological changes, significantly increased Ki67 proliferation, and reduced apoptosis in rat prostate tissues.
34707774	11	3	theme	process	2205:2211	arg1	regulation					2157:2166	regulation	2157:2166	regulation of oxidative stress and inflammatory process	2157:2211	Our experimental evidence demonstrated that periodontitis might promote BPH development through regulation of oxidative stress and inflammatory process, thus providing new strategies for prevention and treatment of BPH.
34707774	8	4	theme	Bax	1710:1712	arg1	expression					1714:1723	Bax expression	1710:1723	Bax expression	1710:1723	Also, our results showed that periodontal ligation induced increased Bcl-2 protein expression, whereas Bax expression was decreased in BPH rats than in normal rats.
34707774	2	5	theme	molecular	310:318	arg1	links					320:324	molecular links	310:324	molecular links of this relationship	310:345	However, molecular links of this relationship are largely unknown.
34707774	10	6	theme	intracellular	2004:2016	arg1	expression					2018:2027	intracellular expression	2004:2027	intracellular expression of ROS and H2O2 in BPH-1 cells	2004:2058	Furthermore, Porphyromonas gingivalis lipopolysaccharide induced enhanced generation of intracellular expression of ROS and H2O2 in BPH-1 cells.
34707774	1	7	theme	prostatic	207:215	arg1	hyperplasia					217:227	benign prostatic hyperplasia	200:227	benign prostatic hyperplasia (BPH)	200:233	Epidemiological studies demonstrate that men with periodontitis are also susceptible to benign prostatic hyperplasia (BPH) and that periodontal treatment can improve the prostatic symptom.
34707774	1	7	theme	prostatic	207:215	arg1	BPH					230:232	BPH	230:232	BPH	230:232	Epidemiological studies demonstrate that men with periodontitis are also susceptible to benign prostatic hyperplasia (BPH) and that periodontal treatment can improve the prostatic symptom.
34707774	11	8	theme	new	2229:2231	arg1	strategies					2233:2242	new strategies	2229:2242	new strategies for prevention and treatment of BPH	2229:2278	Our experimental evidence demonstrated that periodontitis might promote BPH development through regulation of oxidative stress and inflammatory process, thus providing new strategies for prevention and treatment of BPH.
34707774	6	9	theme	oxidative	1199:1207	arg1	stress					1209:1214	oxidative stress	1199:1214	oxidative stress	1199:1214	To further investigate whether oxidative stress participates in the aggravation of BPH, in vitro models were also conducted to measure the production of intracellular reactive oxygen species (ROS) and hydrogen peroxide (H2O2) concentration.
34707774	2	10	theme	relationship	334:345	arg1	links					320:324	molecular links	310:324	molecular links of this relationship	310:345	However, molecular links of this relationship are largely unknown.
34707774	5	11	theme	prostate	911:918	arg1	index					920:924	prostate index	911:924	prostate index	911:924	Four weeks later, all the rats were sacrificed and the following items were measured: alveolar bone loss and histological examination of periodontal tissues were taken to assess the establishment of periodontitis model, prostate index and histological examination of prostate tissues were taken to test the establishment of the BPH model, inflammatory cytokines in plasma were assessed, and Bax/Bcl-2 proteins related to cell apoptosis were analyzed via western blot analysis.
34707774	10	12	from	generation	1990:1999	arg1	cells					2054:2058	BPH-1 cells	2048:2058	BPH-1 cells	2048:2058	Furthermore, Porphyromonas gingivalis lipopolysaccharide induced enhanced generation of intracellular expression of ROS and H2O2 in BPH-1 cells.
34707774	5	13	theme	related	1101:1107	arg1	proteins					1092:1099	Bax/Bcl-2 proteins	1082:1099	Bax/Bcl-2 proteins related to cell apoptosis	1082:1125	Four weeks later, all the rats were sacrificed and the following items were measured: alveolar bone loss and histological examination of periodontal tissues were taken to assess the establishment of periodontitis model, prostate index and histological examination of prostate tissues were taken to test the establishment of the BPH model, inflammatory cytokines in plasma were assessed, and Bax/Bcl-2 proteins related to cell apoptosis were analyzed via western blot analysis.
34707774	5	14	theme	following	746:754	arg1	items					756:760	the following items	742:760	the following items	742:760	Four weeks later, all the rats were sacrificed and the following items were measured: alveolar bone loss and histological examination of periodontal tissues were taken to assess the establishment of periodontitis model, prostate index and histological examination of prostate tissues were taken to test the establishment of the BPH model, inflammatory cytokines in plasma were assessed, and Bax/Bcl-2 proteins related to cell apoptosis were analyzed via western blot analysis.
34707774	5	15	theme	cell	1112:1115	arg1	apoptosis					1117:1125	cell apoptosis	1112:1125	cell apoptosis	1112:1125	Four weeks later, all the rats were sacrificed and the following items were measured: alveolar bone loss and histological examination of periodontal tissues were taken to assess the establishment of periodontitis model, prostate index and histological examination of prostate tissues were taken to test the establishment of the BPH model, inflammatory cytokines in plasma were assessed, and Bax/Bcl-2 proteins related to cell apoptosis were analyzed via western blot analysis.
34707774	7	16	from	proliferation	1545:1557	arg1	tissues					1598:1604	rat prostate tissues	1585:1604	rat prostate tissues	1585:1604	We found that simultaneous periodontitis and BPH synergistically aggravated prostate histological changes, significantly increased Ki67 proliferation, and reduced apoptosis in rat prostate tissues.
34707774	11	17	theme	oxidative	2171:2179	arg1	stress					2181:2186	oxidative stress	2171:2186	oxidative stress	2171:2186	Our experimental evidence demonstrated that periodontitis might promote BPH development through regulation of oxidative stress and inflammatory process, thus providing new strategies for prevention and treatment of BPH.
34707774	6	18	theme	species	1351:1357	arg1	production					1307:1316	the production	1303:1316	the production of intracellular reactive oxygen species (ROS) and hydrogen peroxide (H2O2) concentration	1303:1406	To further investigate whether oxidative stress participates in the aggravation of BPH, in vitro models were also conducted to measure the production of intracellular reactive oxygen species (ROS) and hydrogen peroxide (H2O2) concentration.
34707774	5	19	theme	prostate	958:965	arg1	tissues					967:973	prostate tissues	958:973	prostate tissues	958:973	Four weeks later, all the rats were sacrificed and the following items were measured: alveolar bone loss and histological examination of periodontal tissues were taken to assess the establishment of periodontitis model, prostate index and histological examination of prostate tissues were taken to test the establishment of the BPH model, inflammatory cytokines in plasma were assessed, and Bax/Bcl-2 proteins related to cell apoptosis were analyzed via western blot analysis.
34707774	9	20	theme	control	1790:1796	arg1	group					1798:1802	the control group	1786:1802	the control group	1786:1802	Compared with the control group, periodontitis and BPH both significantly enhanced inflammatory cytokine levels of TNF-α, IL-6, IL-1β, and CRP.
34707774	6	21	theme	reactive	1335:1342	arg1	species					1351:1357	intracellular reactive oxygen species	1321:1357	intracellular reactive oxygen species (ROS)	1321:1363	To further investigate whether oxidative stress participates in the aggravation of BPH, in vitro models were also conducted to measure the production of intracellular reactive oxygen species (ROS) and hydrogen peroxide (H2O2) concentration.
34707774	6	21	theme	reactive	1335:1342	arg1	ROS					1360:1362	ROS	1360:1362	ROS	1360:1362	To further investigate whether oxidative stress participates in the aggravation of BPH, in vitro models were also conducted to measure the production of intracellular reactive oxygen species (ROS) and hydrogen peroxide (H2O2) concentration.
34707774	5	22	theme	Bax/Bcl-2	1082:1090	arg1	proteins					1092:1099	Bax/Bcl-2 proteins	1082:1099	Bax/Bcl-2 proteins related to cell apoptosis	1082:1125	Four weeks later, all the rats were sacrificed and the following items were measured: alveolar bone loss and histological examination of periodontal tissues were taken to assess the establishment of periodontitis model, prostate index and histological examination of prostate tissues were taken to test the establishment of the BPH model, inflammatory cytokines in plasma were assessed, and Bax/Bcl-2 proteins related to cell apoptosis were analyzed via western blot analysis.
34707774	4	23	from	models	658:663	arg1	rats					668:671	rats	668:671	rats	668:671	For this purpose, ligature-induced periodontitis, testosterone-induced BPH, and the composite models in rats were established.
34707774	5	24	theme	blot	1153:1156	arg1	analysis					1158:1165	western blot analysis	1145:1165	western blot analysis	1145:1165	Four weeks later, all the rats were sacrificed and the following items were measured: alveolar bone loss and histological examination of periodontal tissues were taken to assess the establishment of periodontitis model, prostate index and histological examination of prostate tissues were taken to test the establishment of the BPH model, inflammatory cytokines in plasma were assessed, and Bax/Bcl-2 proteins related to cell apoptosis were analyzed via western blot analysis.
34707774	6	25	theme	concentration	1394:1406	arg1	production					1307:1316	the production	1303:1316	the production of intracellular reactive oxygen species (ROS) and hydrogen peroxide (H2O2) concentration	1303:1406	To further investigate whether oxidative stress participates in the aggravation of BPH, in vitro models were also conducted to measure the production of intracellular reactive oxygen species (ROS) and hydrogen peroxide (H2O2) concentration.
34707774	6	26	theme	peroxide	1378:1385	arg1	concentration					1394:1406	hydrogen peroxide (H2O2) concentration	1369:1406	hydrogen peroxide (H2O2) concentration	1369:1406	To further investigate whether oxidative stress participates in the aggravation of BPH, in vitro models were also conducted to measure the production of intracellular reactive oxygen species (ROS) and hydrogen peroxide (H2O2) concentration.
34707774	5	27	theme	BPH	1019:1021	arg1	model					1023:1027	the BPH model	1015:1027	the BPH model	1015:1027	Four weeks later, all the rats were sacrificed and the following items were measured: alveolar bone loss and histological examination of periodontal tissues were taken to assess the establishment of periodontitis model, prostate index and histological examination of prostate tissues were taken to test the establishment of the BPH model, inflammatory cytokines in plasma were assessed, and Bax/Bcl-2 proteins related to cell apoptosis were analyzed via western blot analysis.
34707774	9	28	theme	cytokine	1868:1875	arg1	levels					1877:1882	inflammatory cytokine levels	1855:1882	inflammatory cytokine levels of TNF-α, IL-6, IL-1β, and CRP	1855:1913	Compared with the control group, periodontitis and BPH both significantly enhanced inflammatory cytokine levels of TNF-α, IL-6, IL-1β, and CRP.
34707774	0	29	theme	Stress	87:92	arg1	Regulation					63:72	Regulation	63:72	Regulation of Oxidative Stress and Inflammation	63:109	Periodontitis Exacerbates Benign Prostatic Hyperplasia through Regulation of Oxidative Stress and Inflammation.
34707774	3	30	theme	periodontitis	443:455	arg1	effects					419:425	the effects	415:425	the effects of experimental periodontitis on the hyperplasia of prostate and whether oxidative stress and inflammation participated in this process	415:561	The goal of the current study was to elucidate the effects of experimental periodontitis on the hyperplasia of prostate and whether oxidative stress and inflammation participated in this process.
34707774	4	31	from	periodontitis	599:611	arg1	rats					668:671	rats	668:671	rats	668:671	For this purpose, ligature-induced periodontitis, testosterone-induced BPH, and the composite models in rats were established.
34707774	1	32	with	men	153:155	arg1	periodontitis					162:174	periodontitis	162:174	periodontitis	162:174	Epidemiological studies demonstrate that men with periodontitis are also susceptible to benign prostatic hyperplasia (BPH) and that periodontal treatment can improve the prostatic symptom.
34707774	1	33	theme	Epidemiological	112:126	arg1	studies					128:134	Epidemiological studies	112:134	Epidemiological studies	112:134	Epidemiological studies demonstrate that men with periodontitis are also susceptible to benign prostatic hyperplasia (BPH) and that periodontal treatment can improve the prostatic symptom.
34707774	3	34	theme	study	392:396	arg1	goal					372:375	The goal	368:375	The goal of the current study	368:396	The goal of the current study was to elucidate the effects of experimental periodontitis on the hyperplasia of prostate and whether oxidative stress and inflammation participated in this process.
34707774	0	35	theme	Benign	26:31	arg1	Hyperplasia					43:53	Benign Prostatic Hyperplasia	26:53	Benign Prostatic Hyperplasia	26:53	Periodontitis Exacerbates Benign Prostatic Hyperplasia through Regulation of Oxidative Stress and Inflammation.
34707774	8	36	theme	normal	1759:1764	arg1	rats					1766:1769	normal rats	1759:1769	normal rats	1759:1769	Also, our results showed that periodontal ligation induced increased Bcl-2 protein expression, whereas Bax expression was decreased in BPH rats than in normal rats.
34707774	1	37	theme	benign	200:205	arg1	hyperplasia					217:227	benign prostatic hyperplasia	200:227	benign prostatic hyperplasia (BPH)	200:233	Epidemiological studies demonstrate that men with periodontitis are also susceptible to benign prostatic hyperplasia (BPH) and that periodontal treatment can improve the prostatic symptom.
34707774	1	37	theme	benign	200:205	arg1	BPH					230:232	BPH	230:232	BPH	230:232	Epidemiological studies demonstrate that men with periodontitis are also susceptible to benign prostatic hyperplasia (BPH) and that periodontal treatment can improve the prostatic symptom.
34707774	3	38	theme	experimental	430:441	arg1	periodontitis					443:455	experimental periodontitis	430:455	experimental periodontitis	430:455	The goal of the current study was to elucidate the effects of experimental periodontitis on the hyperplasia of prostate and whether oxidative stress and inflammation participated in this process.
34707774	7	39	theme	rat	1585:1587	arg1	tissues					1598:1604	rat prostate tissues	1585:1604	rat prostate tissues	1585:1604	We found that simultaneous periodontitis and BPH synergistically aggravated prostate histological changes, significantly increased Ki67 proliferation, and reduced apoptosis in rat prostate tissues.
34707774	5	40	theme	bone	786:789	arg1	loss					791:794	alveolar bone loss	777:794	alveolar bone loss	777:794	Four weeks later, all the rats were sacrificed and the following items were measured: alveolar bone loss and histological examination of periodontal tissues were taken to assess the establishment of periodontitis model, prostate index and histological examination of prostate tissues were taken to test the establishment of the BPH model, inflammatory cytokines in plasma were assessed, and Bax/Bcl-2 proteins related to cell apoptosis were analyzed via western blot analysis.
34707774	5	41	theme	periodontal	828:838	arg1	tissues					840:846	periodontal tissues	828:846	periodontal tissues	828:846	Four weeks later, all the rats were sacrificed and the following items were measured: alveolar bone loss and histological examination of periodontal tissues were taken to assess the establishment of periodontitis model, prostate index and histological examination of prostate tissues were taken to test the establishment of the BPH model, inflammatory cytokines in plasma were assessed, and Bax/Bcl-2 proteins related to cell apoptosis were analyzed via western blot analysis.
34707774	8	42	theme	periodontal	1637:1647	arg1	ligation					1649:1656	periodontal ligation	1637:1656	periodontal ligation	1637:1656	Also, our results showed that periodontal ligation induced increased Bcl-2 protein expression, whereas Bax expression was decreased in BPH rats than in normal rats.
34707774	10	43	theme	Porphyromonas	1929:1941	arg1	lipopolysaccharide					1954:1971	Porphyromonas gingivalis lipopolysaccharide	1929:1971	Porphyromonas gingivalis lipopolysaccharide	1929:1971	Furthermore, Porphyromonas gingivalis lipopolysaccharide induced enhanced generation of intracellular expression of ROS and H2O2 in BPH-1 cells.
34707774	3	44	theme	oxidative	500:508	arg1	stress					510:515	oxidative stress	500:515	oxidative stress	500:515	The goal of the current study was to elucidate the effects of experimental periodontitis on the hyperplasia of prostate and whether oxidative stress and inflammation participated in this process.
34707774	1	45	theme	periodontal	244:254	arg1	treatment					256:264	periodontal treatment	244:264	periodontal treatment	244:264	Epidemiological studies demonstrate that men with periodontitis are also susceptible to benign prostatic hyperplasia (BPH) and that periodontal treatment can improve the prostatic symptom.
34707774	9	46	theme	CRP	1911:1913	arg1	levels					1877:1882	inflammatory cytokine levels	1855:1882	inflammatory cytokine levels of TNF-α, IL-6, IL-1β, and CRP	1855:1913	Compared with the control group, periodontitis and BPH both significantly enhanced inflammatory cytokine levels of TNF-α, IL-6, IL-1β, and CRP.
34707774	5	47	theme	model	904:908	arg1	establishment					873:885	the establishment	869:885	the establishment of periodontitis model	869:908	Four weeks later, all the rats were sacrificed and the following items were measured: alveolar bone loss and histological examination of periodontal tissues were taken to assess the establishment of periodontitis model, prostate index and histological examination of prostate tissues were taken to test the establishment of the BPH model, inflammatory cytokines in plasma were assessed, and Bax/Bcl-2 proteins related to cell apoptosis were analyzed via western blot analysis.
34707774	8	48	theme	Bcl-2	1676:1680	arg1	expression					1690:1699	increased Bcl-2 protein expression	1666:1699	increased Bcl-2 protein expression	1666:1699	Also, our results showed that periodontal ligation induced increased Bcl-2 protein expression, whereas Bax expression was decreased in BPH rats than in normal rats.
34707774	10	49	theme	enhanced	1981:1988	arg1	generation					1990:1999	enhanced generation	1981:1999	enhanced generation of intracellular expression of ROS and H2O2 in BPH-1 cells	1981:2058	Furthermore, Porphyromonas gingivalis lipopolysaccharide induced enhanced generation of intracellular expression of ROS and H2O2 in BPH-1 cells.
34707774	6	50	theme	intracellular	1321:1333	arg1	species					1351:1357	intracellular reactive oxygen species	1321:1357	intracellular reactive oxygen species (ROS)	1321:1363	To further investigate whether oxidative stress participates in the aggravation of BPH, in vitro models were also conducted to measure the production of intracellular reactive oxygen species (ROS) and hydrogen peroxide (H2O2) concentration.
34707774	6	50	theme	intracellular	1321:1333	arg1	ROS					1360:1362	ROS	1360:1362	ROS	1360:1362	To further investigate whether oxidative stress participates in the aggravation of BPH, in vitro models were also conducted to measure the production of intracellular reactive oxygen species (ROS) and hydrogen peroxide (H2O2) concentration.
34707774	8	51	theme	BPH	1742:1744	arg1	rats					1746:1749	BPH rats	1742:1749	BPH rats	1742:1749	Also, our results showed that periodontal ligation induced increased Bcl-2 protein expression, whereas Bax expression was decreased in BPH rats than in normal rats.
34707774	7	52	theme	increased	1530:1538	arg1	proliferation					1545:1557	significantly increased Ki67 proliferation	1516:1557	significantly increased Ki67 proliferation	1516:1557	We found that simultaneous periodontitis and BPH synergistically aggravated prostate histological changes, significantly increased Ki67 proliferation, and reduced apoptosis in rat prostate tissues.
34707774	4	53	theme	testosterone-induced	614:633	arg1	BPH					635:637	testosterone-induced BPH	614:637	testosterone-induced BPH	614:637	For this purpose, ligature-induced periodontitis, testosterone-induced BPH, and the composite models in rats were established.
34707774	7	54	theme	prostate	1485:1492	arg1	changes					1507:1513	prostate histological changes	1485:1513	prostate histological changes	1485:1513	We found that simultaneous periodontitis and BPH synergistically aggravated prostate histological changes, significantly increased Ki67 proliferation, and reduced apoptosis in rat prostate tissues.
34707774	10	55	theme	expression	2018:2027	arg1	generation					1990:1999	enhanced generation	1981:1999	enhanced generation of intracellular expression of ROS and H2O2 in BPH-1 cells	1981:2058	Furthermore, Porphyromonas gingivalis lipopolysaccharide induced enhanced generation of intracellular expression of ROS and H2O2 in BPH-1 cells.
34707774	11	56	theme	experimental	2065:2076	arg1	evidence					2078:2085	Our experimental evidence	2061:2085	Our experimental evidence	2061:2085	Our experimental evidence demonstrated that periodontitis might promote BPH development through regulation of oxidative stress and inflammatory process, thus providing new strategies for prevention and treatment of BPH.
34707774	5	57	from	cytokines	1043:1051	arg1	plasma					1056:1061	plasma	1056:1061	plasma	1056:1061	Four weeks later, all the rats were sacrificed and the following items were measured: alveolar bone loss and histological examination of periodontal tissues were taken to assess the establishment of periodontitis model, prostate index and histological examination of prostate tissues were taken to test the establishment of the BPH model, inflammatory cytokines in plasma were assessed, and Bax/Bcl-2 proteins related to cell apoptosis were analyzed via western blot analysis.
34707774	5	58	theme	periodontitis	890:902	arg1	model					904:908	periodontitis model	890:908	periodontitis model	890:908	Four weeks later, all the rats were sacrificed and the following items were measured: alveolar bone loss and histological examination of periodontal tissues were taken to assess the establishment of periodontitis model, prostate index and histological examination of prostate tissues were taken to test the establishment of the BPH model, inflammatory cytokines in plasma were assessed, and Bax/Bcl-2 proteins related to cell apoptosis were analyzed via western blot analysis.
34707774	7	59	theme	histological	1494:1505	arg1	changes					1507:1513	prostate histological changes	1485:1513	prostate histological changes	1485:1513	We found that simultaneous periodontitis and BPH synergistically aggravated prostate histological changes, significantly increased Ki67 proliferation, and reduced apoptosis in rat prostate tissues.
34707774	7	60	from	changes	1507:1513	arg1	tissues					1598:1604	rat prostate tissues	1585:1604	rat prostate tissues	1585:1604	We found that simultaneous periodontitis and BPH synergistically aggravated prostate histological changes, significantly increased Ki67 proliferation, and reduced apoptosis in rat prostate tissues.
34707774	10	61	theme	H2O2	2040:2043	arg1	expression					2018:2027	intracellular expression	2004:2027	intracellular expression of ROS and H2O2 in BPH-1 cells	2004:2058	Furthermore, Porphyromonas gingivalis lipopolysaccharide induced enhanced generation of intracellular expression of ROS and H2O2 in BPH-1 cells.
34707774	6	62	theme	hydrogen	1369:1376	arg1	H2O2					1388:1391	H2O2	1388:1391	H2O2	1388:1391	To further investigate whether oxidative stress participates in the aggravation of BPH, in vitro models were also conducted to measure the production of intracellular reactive oxygen species (ROS) and hydrogen peroxide (H2O2) concentration.
34707774	6	62	theme	hydrogen	1369:1376	arg1	peroxide					1378:1385	hydrogen peroxide	1369:1385	hydrogen peroxide (H2O2) concentration	1369:1406	To further investigate whether oxidative stress participates in the aggravation of BPH, in vitro models were also conducted to measure the production of intracellular reactive oxygen species (ROS) and hydrogen peroxide (H2O2) concentration.
34707774	11	63	theme	BPH	2133:2135	arg1	development					2137:2147	BPH development	2133:2147	BPH development	2133:2147	Our experimental evidence demonstrated that periodontitis might promote BPH development through regulation of oxidative stress and inflammatory process, thus providing new strategies for prevention and treatment of BPH.
34707774	7	64	theme	simultaneous	1423:1434	arg1	periodontitis					1436:1448	simultaneous periodontitis	1423:1448	simultaneous periodontitis	1423:1448	We found that simultaneous periodontitis and BPH synergistically aggravated prostate histological changes, significantly increased Ki67 proliferation, and reduced apoptosis in rat prostate tissues.
34707774	10	65	theme	ROS	2032:2034	arg1	expression					2018:2027	intracellular expression	2004:2027	intracellular expression of ROS and H2O2 in BPH-1 cells	2004:2058	Furthermore, Porphyromonas gingivalis lipopolysaccharide induced enhanced generation of intracellular expression of ROS and H2O2 in BPH-1 cells.
34707774	10	66	from	expression	2018:2027	arg1	cells					2054:2058	BPH-1 cells	2048:2058	BPH-1 cells	2048:2058	Furthermore, Porphyromonas gingivalis lipopolysaccharide induced enhanced generation of intracellular expression of ROS and H2O2 in BPH-1 cells.
34707774	11	67	theme	inflammatory	2192:2203	arg1	process					2205:2211	inflammatory process	2192:2211	inflammatory process	2192:2211	Our experimental evidence demonstrated that periodontitis might promote BPH development through regulation of oxidative stress and inflammatory process, thus providing new strategies for prevention and treatment of BPH.
34707774	6	68	theme	in	1256:1257	arg1	models					1265:1270	in vitro models	1256:1270	in vitro models	1256:1270	To further investigate whether oxidative stress participates in the aggravation of BPH, in vitro models were also conducted to measure the production of intracellular reactive oxygen species (ROS) and hydrogen peroxide (H2O2) concentration.
34707774	10	69	theme	BPH-1	2048:2052	arg1	cells					2054:2058	BPH-1 cells	2048:2058	BPH-1 cells	2048:2058	Furthermore, Porphyromonas gingivalis lipopolysaccharide induced enhanced generation of intracellular expression of ROS and H2O2 in BPH-1 cells.
34707774	6	70	theme	oxygen	1344:1349	arg1	species					1351:1357	intracellular reactive oxygen species	1321:1357	intracellular reactive oxygen species (ROS)	1321:1363	To further investigate whether oxidative stress participates in the aggravation of BPH, in vitro models were also conducted to measure the production of intracellular reactive oxygen species (ROS) and hydrogen peroxide (H2O2) concentration.
34707774	6	70	theme	oxygen	1344:1349	arg1	ROS					1360:1362	ROS	1360:1362	ROS	1360:1362	To further investigate whether oxidative stress participates in the aggravation of BPH, in vitro models were also conducted to measure the production of intracellular reactive oxygen species (ROS) and hydrogen peroxide (H2O2) concentration.
34707774	5	71	theme	tissues	967:973	arg1	index					920:924	prostate index	911:924	prostate index	911:924	Four weeks later, all the rats were sacrificed and the following items were measured: alveolar bone loss and histological examination of periodontal tissues were taken to assess the establishment of periodontitis model, prostate index and histological examination of prostate tissues were taken to test the establishment of the BPH model, inflammatory cytokines in plasma were assessed, and Bax/Bcl-2 proteins related to cell apoptosis were analyzed via western blot analysis.
34707774	5	71	theme	tissues	967:973	arg1	examination					943:953	histological examination	930:953	histological examination	930:953	Four weeks later, all the rats were sacrificed and the following items were measured: alveolar bone loss and histological examination of periodontal tissues were taken to assess the establishment of periodontitis model, prostate index and histological examination of prostate tissues were taken to test the establishment of the BPH model, inflammatory cytokines in plasma were assessed, and Bax/Bcl-2 proteins related to cell apoptosis were analyzed via western blot analysis.
34707774	11	72	theme	stress	2181:2186	arg1	regulation					2157:2166	regulation	2157:2166	regulation of oxidative stress and inflammatory process	2157:2211	Our experimental evidence demonstrated that periodontitis might promote BPH development through regulation of oxidative stress and inflammatory process, thus providing new strategies for prevention and treatment of BPH.
34707774	6	73	theme	BPH	1251:1253	arg1	aggravation					1236:1246	the aggravation	1232:1246	the aggravation of BPH	1232:1253	To further investigate whether oxidative stress participates in the aggravation of BPH, in vitro models were also conducted to measure the production of intracellular reactive oxygen species (ROS) and hydrogen peroxide (H2O2) concentration.
34707774	5	74	theme	alveolar	777:784	arg1	loss					791:794	alveolar bone loss	777:794	alveolar bone loss	777:794	Four weeks later, all the rats were sacrificed and the following items were measured: alveolar bone loss and histological examination of periodontal tissues were taken to assess the establishment of periodontitis model, prostate index and histological examination of prostate tissues were taken to test the establishment of the BPH model, inflammatory cytokines in plasma were assessed, and Bax/Bcl-2 proteins related to cell apoptosis were analyzed via western blot analysis.
34707774	6	75	dep	in	1256:1257	arg1	vitro					1259:1263	vitro	1259:1263	vitro	1259:1263	To further investigate whether oxidative stress participates in the aggravation of BPH, in vitro models were also conducted to measure the production of intracellular reactive oxygen species (ROS) and hydrogen peroxide (H2O2) concentration.
34707774	0	76	theme	Inflammation	98:109	arg1	Regulation					63:72	Regulation	63:72	Regulation of Oxidative Stress and Inflammation	63:109	Periodontitis Exacerbates Benign Prostatic Hyperplasia through Regulation of Oxidative Stress and Inflammation.
34707774	3	77	from	effects	419:425	arg1	hyperplasia					464:474	the hyperplasia	460:474	the hyperplasia of prostate	460:486	The goal of the current study was to elucidate the effects of experimental periodontitis on the hyperplasia of prostate and whether oxidative stress and inflammation participated in this process.
34707774	5	78	theme	histological	930:941	arg1	examination					943:953	histological examination	930:953	histological examination	930:953	Four weeks later, all the rats were sacrificed and the following items were measured: alveolar bone loss and histological examination of periodontal tissues were taken to assess the establishment of periodontitis model, prostate index and histological examination of prostate tissues were taken to test the establishment of the BPH model, inflammatory cytokines in plasma were assessed, and Bax/Bcl-2 proteins related to cell apoptosis were analyzed via western blot analysis.
34707774	3	79	theme	current	384:390	arg1	study					392:396	the current study	380:396	the current study	380:396	The goal of the current study was to elucidate the effects of experimental periodontitis on the hyperplasia of prostate and whether oxidative stress and inflammation participated in this process.
34707774	4	80	from	BPH	635:637	arg1	rats					668:671	rats	668:671	rats	668:671	For this purpose, ligature-induced periodontitis, testosterone-induced BPH, and the composite models in rats were established.
34707774	10	81	from	cells	2054:2058	arg1	generation					1990:1999	enhanced generation	1981:1999	enhanced generation of intracellular expression of ROS and H2O2 in BPH-1 cells	1981:2058	Furthermore, Porphyromonas gingivalis lipopolysaccharide induced enhanced generation of intracellular expression of ROS and H2O2 in BPH-1 cells.
34707774	9	82	theme	inflammatory	1855:1866	arg1	levels					1877:1882	inflammatory cytokine levels	1855:1882	inflammatory cytokine levels of TNF-α, IL-6, IL-1β, and CRP	1855:1913	Compared with the control group, periodontitis and BPH both significantly enhanced inflammatory cytokine levels of TNF-α, IL-6, IL-1β, and CRP.
34707774	5	83	theme	tissues	840:846	arg1	loss					791:794	alveolar bone loss	777:794	alveolar bone loss	777:794	Four weeks later, all the rats were sacrificed and the following items were measured: alveolar bone loss and histological examination of periodontal tissues were taken to assess the establishment of periodontitis model, prostate index and histological examination of prostate tissues were taken to test the establishment of the BPH model, inflammatory cytokines in plasma were assessed, and Bax/Bcl-2 proteins related to cell apoptosis were analyzed via western blot analysis.
34707774	5	83	theme	tissues	840:846	arg1	examination					813:823	histological examination	800:823	histological examination	800:823	Four weeks later, all the rats were sacrificed and the following items were measured: alveolar bone loss and histological examination of periodontal tissues were taken to assess the establishment of periodontitis model, prostate index and histological examination of prostate tissues were taken to test the establishment of the BPH model, inflammatory cytokines in plasma were assessed, and Bax/Bcl-2 proteins related to cell apoptosis were analyzed via western blot analysis.
34707774	5	84	theme	western	1145:1151	arg1	analysis					1158:1165	western blot analysis	1145:1165	western blot analysis	1145:1165	Four weeks later, all the rats were sacrificed and the following items were measured: alveolar bone loss and histological examination of periodontal tissues were taken to assess the establishment of periodontitis model, prostate index and histological examination of prostate tissues were taken to test the establishment of the BPH model, inflammatory cytokines in plasma were assessed, and Bax/Bcl-2 proteins related to cell apoptosis were analyzed via western blot analysis.
34707774	11	85	theme	BPH	2276:2278	arg1	treatment					2263:2271	treatment	2263:2271	treatment	2263:2271	Our experimental evidence demonstrated that periodontitis might promote BPH development through regulation of oxidative stress and inflammatory process, thus providing new strategies for prevention and treatment of BPH.
34707774	11	85	theme	BPH	2276:2278	arg1	prevention					2248:2257	prevention	2248:2257	prevention	2248:2257	Our experimental evidence demonstrated that periodontitis might promote BPH development through regulation of oxidative stress and inflammatory process, thus providing new strategies for prevention and treatment of BPH.
34707774	4	86	theme	ligature-induced	582:597	arg1	periodontitis					599:611	ligature-induced periodontitis	582:611	ligature-induced periodontitis	582:611	For this purpose, ligature-induced periodontitis, testosterone-induced BPH, and the composite models in rats were established.
34707774	0	87	theme	Prostatic	33:41	arg1	Hyperplasia					43:53	Benign Prostatic Hyperplasia	26:53	Benign Prostatic Hyperplasia	26:53	Periodontitis Exacerbates Benign Prostatic Hyperplasia through Regulation of Oxidative Stress and Inflammation.
34707774	9	88	theme	TNF-α	1887:1891	arg1	levels					1877:1882	inflammatory cytokine levels	1855:1882	inflammatory cytokine levels of TNF-α, IL-6, IL-1β, and CRP	1855:1913	Compared with the control group, periodontitis and BPH both significantly enhanced inflammatory cytokine levels of TNF-α, IL-6, IL-1β, and CRP.
34707774	4	89	theme	composite	648:656	arg1	models					658:663	the composite models	644:663	the composite models	644:663	For this purpose, ligature-induced periodontitis, testosterone-induced BPH, and the composite models in rats were established.
34707774	5	90	theme	histological	800:811	arg1	examination					813:823	histological examination	800:823	histological examination	800:823	Four weeks later, all the rats were sacrificed and the following items were measured: alveolar bone loss and histological examination of periodontal tissues were taken to assess the establishment of periodontitis model, prostate index and histological examination of prostate tissues were taken to test the establishment of the BPH model, inflammatory cytokines in plasma were assessed, and Bax/Bcl-2 proteins related to cell apoptosis were analyzed via western blot analysis.
34707774	9	91	theme	IL-6	1894:1897	arg1	levels					1877:1882	inflammatory cytokine levels	1855:1882	inflammatory cytokine levels of TNF-α, IL-6, IL-1β, and CRP	1855:1913	Compared with the control group, periodontitis and BPH both significantly enhanced inflammatory cytokine levels of TNF-α, IL-6, IL-1β, and CRP.
34707774	3	92	theme	prostate	479:486	arg1	hyperplasia					464:474	the hyperplasia	460:474	the hyperplasia of prostate	460:486	The goal of the current study was to elucidate the effects of experimental periodontitis on the hyperplasia of prostate and whether oxidative stress and inflammation participated in this process.
34707774	7	93	theme	prostate	1589:1596	arg1	tissues					1598:1604	rat prostate tissues	1585:1604	rat prostate tissues	1585:1604	We found that simultaneous periodontitis and BPH synergistically aggravated prostate histological changes, significantly increased Ki67 proliferation, and reduced apoptosis in rat prostate tissues.
34707774	0	94	theme	Oxidative	77:85	arg1	Stress					87:92	Oxidative Stress	77:92	Oxidative Stress	77:92	Periodontitis Exacerbates Benign Prostatic Hyperplasia through Regulation of Oxidative Stress and Inflammation.
34707774	9	95	theme	IL-1β	1900:1904	arg1	levels					1877:1882	inflammatory cytokine levels	1855:1882	inflammatory cytokine levels of TNF-α, IL-6, IL-1β, and CRP	1855:1913	Compared with the control group, periodontitis and BPH both significantly enhanced inflammatory cytokine levels of TNF-α, IL-6, IL-1β, and CRP.
34707774	10	96	theme	gingivalis	1943:1952	arg1	lipopolysaccharide					1954:1971	Porphyromonas gingivalis lipopolysaccharide	1929:1971	Porphyromonas gingivalis lipopolysaccharide	1929:1971	Furthermore, Porphyromonas gingivalis lipopolysaccharide induced enhanced generation of intracellular expression of ROS and H2O2 in BPH-1 cells.
34707774	5	97	theme	model	1023:1027	arg1	establishment					998:1010	the establishment	994:1010	the establishment of the BPH model	994:1027	Four weeks later, all the rats were sacrificed and the following items were measured: alveolar bone loss and histological examination of periodontal tissues were taken to assess the establishment of periodontitis model, prostate index and histological examination of prostate tissues were taken to test the establishment of the BPH model, inflammatory cytokines in plasma were assessed, and Bax/Bcl-2 proteins related to cell apoptosis were analyzed via western blot analysis.
34707774	8	98	theme	increased	1666:1674	arg1	expression					1690:1699	increased Bcl-2 protein expression	1666:1699	increased Bcl-2 protein expression	1666:1699	Also, our results showed that periodontal ligation induced increased Bcl-2 protein expression, whereas Bax expression was decreased in BPH rats than in normal rats.
34707774	7	99	theme	reduced	1564:1570	arg1	apoptosis					1572:1580	reduced apoptosis	1564:1580	reduced apoptosis in rat prostate tissues	1564:1604	We found that simultaneous periodontitis and BPH synergistically aggravated prostate histological changes, significantly increased Ki67 proliferation, and reduced apoptosis in rat prostate tissues.
34707774	5	100	theme	inflammatory	1030:1041	arg1	cytokines					1043:1051	inflammatory cytokines	1030:1051	inflammatory cytokines in plasma	1030:1061	Four weeks later, all the rats were sacrificed and the following items were measured: alveolar bone loss and histological examination of periodontal tissues were taken to assess the establishment of periodontitis model, prostate index and histological examination of prostate tissues were taken to test the establishment of the BPH model, inflammatory cytokines in plasma were assessed, and Bax/Bcl-2 proteins related to cell apoptosis were analyzed via western blot analysis.
34707774	8	101	theme	protein	1682:1688	arg1	expression					1690:1699	increased Bcl-2 protein expression	1666:1699	increased Bcl-2 protein expression	1666:1699	Also, our results showed that periodontal ligation induced increased Bcl-2 protein expression, whereas Bax expression was decreased in BPH rats than in normal rats.
33467664	4	0	from	gain	548:551	arg1	bees					621:624	honey bees	615:624	honey bees	615:624	Excess dietary fat, especially palm oil, elicited higher weight gain, lower survival rates, hyperglycemic, and fat accumulation in honey bees.
33467664	8	1	theme	signaling	1092:1100	arg1	pathways					1102:1109	Toll and Imd signaling pathways	1079:1109	pathways	1102:1109	Gene set enrichment analysis showed that biological processes, including transcription factors, insulin secretion, and Toll and Imd signaling pathways, were significantly different in the gut of bees on different dietary fats.
33467664	8	2	theme	enrichment	969:978	arg1	analysis					980:987	Gene set enrichment analysis	960:987	Gene set enrichment analysis	960:987	Gene set enrichment analysis showed that biological processes, including transcription factors, insulin secretion, and Toll and Imd signaling pathways, were significantly different in the gut of bees on different dietary fats.
33467664	9	3	theme	oil	1328:1330	arg1	groups					1332:1337	palm oil groups	1323:1337	palm oil groups	1323:1337	Moreover, a high-fat diet increased the relative abundance of Gilliamella, while the level of Bartonella was significantly decreased in palm oil groups.
33467664	0	4	theme	Gut	72:74	arg1	Microbiota					76:85	Perturb Gut Microbiota	64:85	Perturb Gut Microbiota	64:85	High-Fat Diets with Differential Fatty Acids Induce Obesity and Perturb Gut Microbiota in Honey Bee.
33467664	6	5	theme	body	837:840	arg1	profiles					811:818	the lipid profiles	801:818	the lipid profiles of the honey bee body	801:840	Different fatty acid compositions in palm and soybean oil altered the lipid profiles of the honey bee body.
33467664	8	6	theme	set	965:967	arg1	analysis					980:987	Gene set enrichment analysis	960:987	Gene set enrichment analysis	960:987	Gene set enrichment analysis showed that biological processes, including transcription factors, insulin secretion, and Toll and Imd signaling pathways, were significantly different in the gut of bees on different dietary fats.
33467664	10	7	theme	dietary	1421:1427	arg1	fat					1429:1431	dietary fat	1421:1431	dietary fat	1421:1431	This study establishes a novel honey bee model of studying the crosstalk between dietary fat, gut microbiota, and host metabolism.
33467664	10	8	theme	model	1381:1385	arg1	bee					1377:1379	a novel honey bee	1363:1379	a novel honey bee model of studying the crosstalk between dietary fat, gut microbiota, and host metabolism	1363:1468	This study establishes a novel honey bee model of studying the crosstalk between dietary fat, gut microbiota, and host metabolism.
33467664	0	9	theme	Honey	90:94	arg1	Bee					96:98	Honey Bee	90:98	Honey Bee	90:98	High-Fat Diets with Differential Fatty Acids Induce Obesity and Perturb Gut Microbiota in Honey Bee.
33467664	6	10	theme	bee	833:835	arg1	body					837:840	the honey bee body	823:840	the honey bee body	823:840	Different fatty acid compositions in palm and soybean oil altered the lipid profiles of the honey bee body.
33467664	10	11	theme	honey	1371:1375	arg1	bee					1377:1379	a novel honey bee	1363:1379	a novel honey bee model of studying the crosstalk between dietary fat, gut microbiota, and host metabolism	1363:1468	This study establishes a novel honey bee model of studying the crosstalk between dietary fat, gut microbiota, and host metabolism.
33467664	6	12	theme	acid	751:754	arg1	compositions					756:767	Different fatty acid compositions	735:767	Different fatty acid compositions in palm and soybean oil	735:791	Different fatty acid compositions in palm and soybean oil altered the lipid profiles of the honey bee body.
33467664	4	13	theme	higher	534:539	arg1	gain					548:551	higher weight gain	534:551	higher weight gain	534:551	Excess dietary fat, especially palm oil, elicited higher weight gain, lower survival rates, hyperglycemic, and fat accumulation in honey bees.
33467664	6	14	theme	honey	827:831	arg1	bee					833:835	the honey bee	823:835	the honey bee body	823:840	Different fatty acid compositions in palm and soybean oil altered the lipid profiles of the honey bee body.
33467664	10	15	theme	novel	1365:1369	arg1	bee					1377:1379	a novel honey bee	1363:1379	a novel honey bee model of studying the crosstalk between dietary fat, gut microbiota, and host metabolism	1363:1468	This study establishes a novel honey bee model of studying the crosstalk between dietary fat, gut microbiota, and host metabolism.
33467664	6	16	theme	fatty	745:749	arg1	compositions					756:767	Different fatty acid compositions	735:767	Different fatty acid compositions in palm and soybean oil	735:791	Different fatty acid compositions in palm and soybean oil altered the lipid profiles of the honey bee body.
33467664	2	17	theme	molecular	254:262	arg1	mechanisms					264:273	the underlying molecular mechanisms	239:273	the underlying molecular mechanisms of the processes	239:290	However, the underlying molecular mechanisms of the processes are poorly understood.
33467664	4	18	from	accumulation	599:610	arg1	bees					621:624	honey bees	615:624	honey bees	615:624	Excess dietary fat, especially palm oil, elicited higher weight gain, lower survival rates, hyperglycemic, and fat accumulation in honey bees.
33467664	9	19	theme	Gilliamella	1249:1259	arg1	abundance					1236:1244	the relative abundance	1223:1244	the relative abundance of Gilliamella	1223:1259	Moreover, a high-fat diet increased the relative abundance of Gilliamella, while the level of Bartonella was significantly decreased in palm oil groups.
33467664	8	20	theme	biological	1001:1010	arg1	factors					1047:1053	transcription factors	1033:1053	transcription factors	1033:1053	Gene set enrichment analysis showed that biological processes, including transcription factors, insulin secretion, and Toll and Imd signaling pathways, were significantly different in the gut of bees on different dietary fats.
33467664	8	20	theme	biological	1001:1010	arg1	secretion					1064:1072	insulin secretion	1056:1072	insulin secretion	1056:1072	Gene set enrichment analysis showed that biological processes, including transcription factors, insulin secretion, and Toll and Imd signaling pathways, were significantly different in the gut of bees on different dietary fats.
33467664	8	20	theme	biological	1001:1010	arg1	processes					1012:1020	biological processes	1001:1020	biological processes	1001:1020	Gene set enrichment analysis showed that biological processes, including transcription factors, insulin secretion, and Toll and Imd signaling pathways, were significantly different in the gut of bees on different dietary fats.
33467664	8	20	theme	biological	1001:1010	arg1	pathways					1102:1109	Toll and Imd signaling pathways	1079:1109	pathways	1102:1109	Gene set enrichment analysis showed that biological processes, including transcription factors, insulin secretion, and Toll and Imd signaling pathways, were significantly different in the gut of bees on different dietary fats.
33467664	4	21	theme	survival	560:567	arg1	rates					569:573	lower survival rates	554:573	lower survival rates	554:573	Excess dietary fat, especially palm oil, elicited higher weight gain, lower survival rates, hyperglycemic, and fat accumulation in honey bees.
33467664	2	22	theme	underlying	243:252	arg1	mechanisms					264:273	the underlying molecular mechanisms	239:273	the underlying molecular mechanisms of the processes	239:290	However, the underlying molecular mechanisms of the processes are poorly understood.
33467664	1	23	theme	metabolic	141:149	arg1	disorders					151:159	metabolic disorders	141:159	metabolic disorders	141:159	HFD (high-fat diet) induces obesity and metabolic disorders, which is associated with the alteration in gut microbiota profiles.
33467664	5	24	theme	high-fat	673:680	arg1	diets					682:686	high-fat diets	673:686	high-fat diets	673:686	However, microbiota-free honey bees reared on high-fat diets did not significantly change their phenotypes.
33467664	0	25	theme	High-Fat	0:7	arg1	Diets					9:13	High-Fat Diets	0:13	High-Fat Diets with Differential Fatty Acids	0:43	High-Fat Diets with Differential Fatty Acids Induce Obesity and Perturb Gut Microbiota in Honey Bee.
33467664	8	26	theme	dietary	1173:1179	arg1	fats					1181:1184	different dietary fats	1163:1184	different dietary fats	1163:1184	Gene set enrichment analysis showed that biological processes, including transcription factors, insulin secretion, and Toll and Imd signaling pathways, were significantly different in the gut of bees on different dietary fats.
33467664	3	27	theme	simple	342:347	arg1	organism					355:362	the simple model organism	338:362	the simple model organism honey bee	338:372	In this study, we used the simple model organism honey bee to explore how different amounts and types of dietary fats affect the host metabolism and the gut microbiota.
33467664	8	28	theme	Imd	1088:1090	arg1	pathways					1102:1109	Toll and Imd signaling pathways	1079:1109	pathways	1102:1109	Gene set enrichment analysis showed that biological processes, including transcription factors, insulin secretion, and Toll and Imd signaling pathways, were significantly different in the gut of bees on different dietary fats.
33467664	7	29	theme	dietary	855:861	arg1	fats					863:866	dietary fats	855:866	dietary fats	855:866	Remarkably, dietary fats regulated lipid metabolism and immune-related gene expression at the transcriptional level.
33467664	6	30	from	compositions	756:767	arg1	palm					772:775	palm	772:775	palm	772:775	Different fatty acid compositions in palm and soybean oil altered the lipid profiles of the honey bee body.
33467664	6	30	from	compositions	756:767	arg1	oil					789:791	soybean oil	781:791	soybean oil	781:791	Different fatty acid compositions in palm and soybean oil altered the lipid profiles of the honey bee body.
33467664	3	31	theme	model	349:353	arg1	organism					355:362	the simple model organism	338:362	the simple model organism honey bee	338:372	In this study, we used the simple model organism honey bee to explore how different amounts and types of dietary fats affect the host metabolism and the gut microbiota.
33467664	8	32	theme	Gene	960:963	arg1	analysis					980:987	Gene set enrichment analysis	960:987	Gene set enrichment analysis	960:987	Gene set enrichment analysis showed that biological processes, including transcription factors, insulin secretion, and Toll and Imd signaling pathways, were significantly different in the gut of bees on different dietary fats.
33467664	2	33	theme	processes	282:290	arg1	mechanisms					264:273	the underlying molecular mechanisms	239:273	the underlying molecular mechanisms of the processes	239:290	However, the underlying molecular mechanisms of the processes are poorly understood.
33467664	4	34	from	hyperglycemic	576:588	arg1	bees					621:624	honey bees	615:624	honey bees	615:624	Excess dietary fat, especially palm oil, elicited higher weight gain, lower survival rates, hyperglycemic, and fat accumulation in honey bees.
33467664	4	35	theme	fat	595:597	arg1	accumulation					599:610	fat accumulation	595:610	fat accumulation in honey bees	595:624	Excess dietary fat, especially palm oil, elicited higher weight gain, lower survival rates, hyperglycemic, and fat accumulation in honey bees.
33467664	0	36	theme	Differential	20:31	arg1	Acids					39:43	Differential Fatty Acids	20:43	Differential Fatty Acids	20:43	High-Fat Diets with Differential Fatty Acids Induce Obesity and Perturb Gut Microbiota in Honey Bee.
33467664	7	37	theme	lipid	878:882	arg1	metabolism					884:893	lipid metabolism	878:893	lipid metabolism	878:893	Remarkably, dietary fats regulated lipid metabolism and immune-related gene expression at the transcriptional level.
33467664	8	38	theme	transcription	1033:1045	arg1	factors					1047:1053	transcription factors	1033:1053	transcription factors	1033:1053	Gene set enrichment analysis showed that biological processes, including transcription factors, insulin secretion, and Toll and Imd signaling pathways, were significantly different in the gut of bees on different dietary fats.
33467664	6	39	theme	Different	735:743	arg1	compositions					756:767	Different fatty acid compositions	735:767	Different fatty acid compositions in palm and soybean oil	735:791	Different fatty acid compositions in palm and soybean oil altered the lipid profiles of the honey bee body.
33467664	4	40	theme	palm	515:518	arg1	oil					520:522	palm oil	515:522	especially palm oil	504:522	Excess dietary fat, especially palm oil, elicited higher weight gain, lower survival rates, hyperglycemic, and fat accumulation in honey bees.
33467664	4	40	theme	palm	515:518	arg1	fat					499:501	Excess dietary fat	484:501	Excess dietary fat	484:501	Excess dietary fat, especially palm oil, elicited higher weight gain, lower survival rates, hyperglycemic, and fat accumulation in honey bees.
33467664	3	41	used	used	333:336	arg2	we					330:331	we	330:331	we	330:331	In this study, we used the simple model organism honey bee to explore how different amounts and types of dietary fats affect the host metabolism and the gut microbiota.
33467664	6	42	theme	lipid	805:809	arg1	profiles					811:818	the lipid profiles	801:818	the lipid profiles of the honey bee body	801:840	Different fatty acid compositions in palm and soybean oil altered the lipid profiles of the honey bee body.
33467664	4	43	from	rates	569:573	arg1	bees					621:624	honey bees	615:624	honey bees	615:624	Excess dietary fat, especially palm oil, elicited higher weight gain, lower survival rates, hyperglycemic, and fat accumulation in honey bees.
33467664	3	44	theme	dietary	420:426	arg1	fats					428:431	dietary fats	420:431	dietary fats	420:431	In this study, we used the simple model organism honey bee to explore how different amounts and types of dietary fats affect the host metabolism and the gut microbiota.
33467664	8	45	theme	bees	1155:1158	arg1	gut					1148:1150	the gut	1144:1150	the gut of bees on different dietary fats	1144:1184	Gene set enrichment analysis showed that biological processes, including transcription factors, insulin secretion, and Toll and Imd signaling pathways, were significantly different in the gut of bees on different dietary fats.
33467664	9	46	theme	Bartonella	1281:1290	arg1	level					1272:1276	the level	1268:1276	the level of Bartonella	1268:1290	Moreover, a high-fat diet increased the relative abundance of Gilliamella, while the level of Bartonella was significantly decreased in palm oil groups.
33467664	5	47	theme	microbiota-free	636:650	arg1	bees					658:661	microbiota-free honey bees	636:661	microbiota-free honey bees reared on high-fat diets	636:686	However, microbiota-free honey bees reared on high-fat diets did not significantly change their phenotypes.
33467664	8	48	from	different	1131:1139	arg1	gut					1148:1150	the gut	1144:1150	the gut of bees on different dietary fats	1144:1184	Gene set enrichment analysis showed that biological processes, including transcription factors, insulin secretion, and Toll and Imd signaling pathways, were significantly different in the gut of bees on different dietary fats.
33467664	0	49	theme	Fatty	33:37	arg1	Acids					39:43	Differential Fatty Acids	20:43	Differential Fatty Acids	20:43	High-Fat Diets with Differential Fatty Acids Induce Obesity and Perturb Gut Microbiota in Honey Bee.
33467664	5	50	theme	honey	652:656	arg1	bees					658:661	microbiota-free honey bees	636:661	microbiota-free honey bees reared on high-fat diets	636:686	However, microbiota-free honey bees reared on high-fat diets did not significantly change their phenotypes.
33467664	4	51	theme	lower	554:558	arg1	rates					569:573	lower survival rates	554:573	lower survival rates	554:573	Excess dietary fat, especially palm oil, elicited higher weight gain, lower survival rates, hyperglycemic, and fat accumulation in honey bees.
33467664	10	52	theme	host	1454:1457	arg1	metabolism					1459:1468	host metabolism	1454:1468	host metabolism	1454:1468	This study establishes a novel honey bee model of studying the crosstalk between dietary fat, gut microbiota, and host metabolism.
33467664	3	53	theme	organism	355:362	arg1	bee					370:372	the simple model organism honey bee	338:372	the simple model organism honey bee	338:372	In this study, we used the simple model organism honey bee to explore how different amounts and types of dietary fats affect the host metabolism and the gut microbiota.
33467664	3	54	theme	different	389:397	arg1	fats					428:431	dietary fats	420:431	dietary fats	420:431	In this study, we used the simple model organism honey bee to explore how different amounts and types of dietary fats affect the host metabolism and the gut microbiota.
33467664	3	54	theme	different	389:397	arg1	amounts					399:405	different amounts	389:405	different amounts	389:405	In this study, we used the simple model organism honey bee to explore how different amounts and types of dietary fats affect the host metabolism and the gut microbiota.
33467664	7	55	theme	transcriptional	937:951	arg1	level					953:957	the transcriptional level	933:957	the transcriptional level	933:957	Remarkably, dietary fats regulated lipid metabolism and immune-related gene expression at the transcriptional level.
33467664	3	56	theme	honey	364:368	arg1	bee					370:372	the simple model organism honey bee	338:372	the simple model organism honey bee	338:372	In this study, we used the simple model organism honey bee to explore how different amounts and types of dietary fats affect the host metabolism and the gut microbiota.
33467664	4	57	theme	dietary	491:497	arg1	oil					520:522	palm oil	515:522	especially palm oil	504:522	Excess dietary fat, especially palm oil, elicited higher weight gain, lower survival rates, hyperglycemic, and fat accumulation in honey bees.
33467664	4	57	theme	dietary	491:497	arg1	fat					499:501	Excess dietary fat	484:501	Excess dietary fat	484:501	Excess dietary fat, especially palm oil, elicited higher weight gain, lower survival rates, hyperglycemic, and fat accumulation in honey bees.
33467664	8	58	theme	insulin	1056:1062	arg1	secretion					1064:1072	insulin secretion	1056:1072	insulin secretion	1056:1072	Gene set enrichment analysis showed that biological processes, including transcription factors, insulin secretion, and Toll and Imd signaling pathways, were significantly different in the gut of bees on different dietary fats.
33467664	6	59	theme	soybean	781:787	arg1	oil					789:791	soybean oil	781:791	soybean oil	781:791	Different fatty acid compositions in palm and soybean oil altered the lipid profiles of the honey bee body.
33467664	0	60	with	Diets	9:13	arg1	Acids					39:43	Differential Fatty Acids	20:43	Differential Fatty Acids	20:43	High-Fat Diets with Differential Fatty Acids Induce Obesity and Perturb Gut Microbiota in Honey Bee.
33467664	3	61	theme	host	444:447	arg1	metabolism					449:458	the host metabolism	440:458	the host metabolism	440:458	In this study, we used the simple model organism honey bee to explore how different amounts and types of dietary fats affect the host metabolism and the gut microbiota.
33467664	4	62	theme	Excess	484:489	arg1	oil					520:522	palm oil	515:522	especially palm oil	504:522	Excess dietary fat, especially palm oil, elicited higher weight gain, lower survival rates, hyperglycemic, and fat accumulation in honey bees.
33467664	4	62	theme	Excess	484:489	arg1	fat					499:501	Excess dietary fat	484:501	Excess dietary fat	484:501	Excess dietary fat, especially palm oil, elicited higher weight gain, lower survival rates, hyperglycemic, and fat accumulation in honey bees.
33467664	1	63	from	alteration	191:200	arg1	profiles					220:227	gut microbiota profiles	205:227	gut microbiota profiles	205:227	HFD (high-fat diet) induces obesity and metabolic disorders, which is associated with the alteration in gut microbiota profiles.
33467664	7	64	theme	immune-related	899:912	arg1	expression					919:928	immune-related gene expression	899:928	immune-related gene expression	899:928	Remarkably, dietary fats regulated lipid metabolism and immune-related gene expression at the transcriptional level.
33467664	1	65	theme	high-fat	106:113	arg1	HFD					101:103	HFD	101:103	HFD (high-fat diet)	101:119	HFD (high-fat diet) induces obesity and metabolic disorders, which is associated with the alteration in gut microbiota profiles.
33467664	1	65	theme	high-fat	106:113	arg1	diet					115:118	high-fat diet	106:118	high-fat diet	106:118	HFD (high-fat diet) induces obesity and metabolic disorders, which is associated with the alteration in gut microbiota profiles.
33467664	7	66	theme	gene	914:917	arg1	expression					919:928	immune-related gene expression	899:928	immune-related gene expression	899:928	Remarkably, dietary fats regulated lipid metabolism and immune-related gene expression at the transcriptional level.
33467664	9	67	theme	palm	1323:1326	arg1	groups					1332:1337	palm oil groups	1323:1337	palm oil groups	1323:1337	Moreover, a high-fat diet increased the relative abundance of Gilliamella, while the level of Bartonella was significantly decreased in palm oil groups.
33467664	10	68	theme	gut	1434:1436	arg1	microbiota					1438:1447	gut microbiota	1434:1447	gut microbiota	1434:1447	This study establishes a novel honey bee model of studying the crosstalk between dietary fat, gut microbiota, and host metabolism.
33467664	9	69	theme	high-fat	1199:1206	arg1	diet					1208:1211	a high-fat diet	1197:1211	a high-fat diet	1197:1211	Moreover, a high-fat diet increased the relative abundance of Gilliamella, while the level of Bartonella was significantly decreased in palm oil groups.
33467664	8	70	theme	different	1163:1171	arg1	fats					1181:1184	different dietary fats	1163:1184	different dietary fats	1163:1184	Gene set enrichment analysis showed that biological processes, including transcription factors, insulin secretion, and Toll and Imd signaling pathways, were significantly different in the gut of bees on different dietary fats.
33467664	3	71	theme	fats	428:431	arg1	types					411:415	types	411:415	types	411:415	In this study, we used the simple model organism honey bee to explore how different amounts and types of dietary fats affect the host metabolism and the gut microbiota.
33467664	3	71	theme	fats	428:431	arg1	fats					428:431	dietary fats	420:431	dietary fats	420:431	In this study, we used the simple model organism honey bee to explore how different amounts and types of dietary fats affect the host metabolism and the gut microbiota.
33467664	3	71	theme	fats	428:431	arg1	amounts					399:405	different amounts	389:405	different amounts	389:405	In this study, we used the simple model organism honey bee to explore how different amounts and types of dietary fats affect the host metabolism and the gut microbiota.
33467664	0	72	theme	Perturb	64:70	arg1	Microbiota					76:85	Perturb Gut Microbiota	64:85	Perturb Gut Microbiota	64:85	High-Fat Diets with Differential Fatty Acids Induce Obesity and Perturb Gut Microbiota in Honey Bee.
33467664	4	73	theme	honey	615:619	arg1	bees					621:624	honey bees	615:624	honey bees	615:624	Excess dietary fat, especially palm oil, elicited higher weight gain, lower survival rates, hyperglycemic, and fat accumulation in honey bees.
33467664	9	74	theme	relative	1227:1234	arg1	abundance					1236:1244	the relative abundance	1223:1244	the relative abundance of Gilliamella	1223:1259	Moreover, a high-fat diet increased the relative abundance of Gilliamella, while the level of Bartonella was significantly decreased in palm oil groups.
33467664	1	75	theme	gut	205:207	arg1	profiles					220:227	gut microbiota profiles	205:227	gut microbiota profiles	205:227	HFD (high-fat diet) induces obesity and metabolic disorders, which is associated with the alteration in gut microbiota profiles.
33467664	8	76	theme	Toll	1079:1082	arg1	pathways					1102:1109	Toll and Imd signaling pathways	1079:1109	pathways	1102:1109	Gene set enrichment analysis showed that biological processes, including transcription factors, insulin secretion, and Toll and Imd signaling pathways, were significantly different in the gut of bees on different dietary fats.
33467664	8	77	from	gut	1148:1150	arg1	different					1131:1139	different	1131:1139	different	1131:1139	Gene set enrichment analysis showed that biological processes, including transcription factors, insulin secretion, and Toll and Imd signaling pathways, were significantly different in the gut of bees on different dietary fats.
33467664	8	77	from	gut	1148:1150	arg1	fats					1181:1184	different dietary fats	1163:1184	different dietary fats	1163:1184	Gene set enrichment analysis showed that biological processes, including transcription factors, insulin secretion, and Toll and Imd signaling pathways, were significantly different in the gut of bees on different dietary fats.
33467664	4	78	theme	weight	541:546	arg1	gain					548:551	higher weight gain	534:551	higher weight gain	534:551	Excess dietary fat, especially palm oil, elicited higher weight gain, lower survival rates, hyperglycemic, and fat accumulation in honey bees.
33467664	3	79	theme	gut	468:470	arg1	microbiota					472:481	the gut microbiota	464:481	the gut microbiota	464:481	In this study, we used the simple model organism honey bee to explore how different amounts and types of dietary fats affect the host metabolism and the gut microbiota.
33467664	1	80	theme	microbiota	209:218	arg1	profiles					220:227	gut microbiota profiles	205:227	gut microbiota profiles	205:227	HFD (high-fat diet) induces obesity and metabolic disorders, which is associated with the alteration in gut microbiota profiles.
32683259	5	0	theme	water	1087:1091	arg1	activity					1093:1100	water activity	1087:1100	water activity	1087:1100	Finally, decreasing the pH led to the decrease of the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity).
32683259	5	1	theme	camel	1030:1034	arg1	temperature					1015:1025	the glass transition temperature	994:1025	the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity)	994:1101	Finally, decreasing the pH led to the decrease of the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity).
32683259	4	2	theme	water	781:785	arg1	isotherms					796:804	the water sorption isotherms	777:804	the water sorption isotherms	777:804	Analysis of the water sorption isotherms indicated that decreasing the pH induced the increase of the water activity of lactose crystallization for camel and bovine whey powders.
32683259	1	3	from	effect	161:166	arg1	temperature					289:299	the glass transition temperature	268:299	the glass transition temperature	268:299	This study investigated the effect of pH on the denaturation extent, the surface chemical composition, the water sorption isotherm and the glass transition temperature of camel and bovine whey protein's powders.
32683259	1	3	from	effect	161:166	arg1	isotherm					255:262	the water sorption isotherm	236:262	the water sorption isotherm	236:262	This study investigated the effect of pH on the denaturation extent, the surface chemical composition, the water sorption isotherm and the glass transition temperature of camel and bovine whey protein's powders.
32683259	1	3	from	effect	161:166	arg1	composition					223:233	the surface chemical composition	202:233	the surface chemical composition	202:233	This study investigated the effect of pH on the denaturation extent, the surface chemical composition, the water sorption isotherm and the glass transition temperature of camel and bovine whey protein's powders.
32683259	1	3	from	effect	161:166	arg1	extent					194:199	the denaturation extent	177:199	the denaturation extent	177:199	This study investigated the effect of pH on the denaturation extent, the surface chemical composition, the water sorption isotherm and the glass transition temperature of camel and bovine whey protein's powders.
32683259	2	4	from	whey	525:528	arg1	absent					509:514	absent	509:514	absent	509:514	The LC-MS analysis indicated that the β-Lactoglobulin was the most denatured protein in bovine whey powders regardless the pH value, while this protein was totally absent in camel whey.
32683259	0	5	theme	chemical	68:75	arg1	composition					77:87	the surface chemical composition	56:87	the surface chemical composition	56:87	Effect of pH on the physicochemical characteristics and the surface chemical composition of camel and bovine whey protein's powders.
32683259	1	6	theme	bovine	314:319	arg1	protein					326:332	bovine whey protein	314:332	bovine whey protein	314:332	This study investigated the effect of pH on the denaturation extent, the surface chemical composition, the water sorption isotherm and the glass transition temperature of camel and bovine whey protein's powders.
32683259	5	7	from	0.13	1063:1066	arg1	camel					1030:1034	camel	1030:1034	camel	1030:1034	Finally, decreasing the pH led to the decrease of the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity).
32683259	5	7	from	0.13	1063:1066	arg1	temperature					1015:1025	the glass transition temperature	994:1025	the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity)	994:1101	Finally, decreasing the pH led to the decrease of the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity).
32683259	5	7	from	0.13	1063:1066	arg1	powder					1052:1057	bovine whey powder	1040:1057	bovine whey powder (at 0.13, 0.23, and 0.33 of water activity)	1040:1101	Finally, decreasing the pH led to the decrease of the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity).
32683259	0	8	from	Effect	0:5	arg1	composition					77:87	the surface chemical composition	56:87	the surface chemical composition	56:87	Effect of pH on the physicochemical characteristics and the surface chemical composition of camel and bovine whey protein's powders.
32683259	0	8	from	Effect	0:5	arg1	characteristics					36:50	the physicochemical characteristics	16:50	the physicochemical characteristics	16:50	Effect of pH on the physicochemical characteristics and the surface chemical composition of camel and bovine whey protein's powders.
32683259	4	9	theme	isotherms	796:804	arg1	Analysis					765:772	Analysis	765:772	Analysis of the water sorption isotherms	765:804	Analysis of the water sorption isotherms indicated that decreasing the pH induced the increase of the water activity of lactose crystallization for camel and bovine whey powders.
32683259	3	10	dep	pH	721:722	arg1	acidic					742:747	acidic	742:747	acidic	742:747	The α-Lactalbumin was relatively heat stable after drying and predominated the powder surface (X-ray photoelectron spectroscopy results) in both camel and bovine whey powders regardless the pH (neutral (6.7) or acidic (4.3 and 4.6)).
32683259	3	10	dep	pH	721:722	arg1	neutral					725:731	neutral	725:731	neutral	725:731	The α-Lactalbumin was relatively heat stable after drying and predominated the powder surface (X-ray photoelectron spectroscopy results) in both camel and bovine whey powders regardless the pH (neutral (6.7) or acidic (4.3 and 4.6)).
32683259	3	10	dep	pH	721:722	arg1	4.6					758:760	4.6	758:760	4.6	758:760	The α-Lactalbumin was relatively heat stable after drying and predominated the powder surface (X-ray photoelectron spectroscopy results) in both camel and bovine whey powders regardless the pH (neutral (6.7) or acidic (4.3 and 4.6)).
32683259	3	10	dep	pH	721:722	arg1	4.3					750:752	4.3	750:752	4.3	750:752	The α-Lactalbumin was relatively heat stable after drying and predominated the powder surface (X-ray photoelectron spectroscopy results) in both camel and bovine whey powders regardless the pH (neutral (6.7) or acidic (4.3 and 4.6)).
32683259	3	10	dep	pH	721:722	arg1	6.7					734:736	6.7	734:736	6.7	734:736	The α-Lactalbumin was relatively heat stable after drying and predominated the powder surface (X-ray photoelectron spectroscopy results) in both camel and bovine whey powders regardless the pH (neutral (6.7) or acidic (4.3 and 4.6)).
32683259	3	11	theme	bovine	686:691	arg1	powders					698:704	both camel and bovine whey powders	671:704	both camel and bovine whey powders	671:704	The α-Lactalbumin was relatively heat stable after drying and predominated the powder surface (X-ray photoelectron spectroscopy results) in both camel and bovine whey powders regardless the pH (neutral (6.7) or acidic (4.3 and 4.6)).
32683259	4	12	theme	sorption	787:794	arg1	isotherms					796:804	the water sorption isotherms	777:804	the water sorption isotherms	777:804	Analysis of the water sorption isotherms indicated that decreasing the pH induced the increase of the water activity of lactose crystallization for camel and bovine whey powders.
32683259	4	13	theme	decreasing	821:830	arg1	pH					836:837	the pH	832:837	decreasing the pH	821:837	Analysis of the water sorption isotherms indicated that decreasing the pH induced the increase of the water activity of lactose crystallization for camel and bovine whey powders.
32683259	1	14	theme	water	240:244	arg1	isotherm					255:262	the water sorption isotherm	236:262	the water sorption isotherm	236:262	This study investigated the effect of pH on the denaturation extent, the surface chemical composition, the water sorption isotherm and the glass transition temperature of camel and bovine whey protein's powders.
32683259	0	15	theme	bovine	102:107	arg1	protein					114:120	bovine whey protein	102:120	bovine whey protein	102:120	Effect of pH on the physicochemical characteristics and the surface chemical composition of camel and bovine whey protein's powders.
32683259	3	16	theme	whey	693:696	arg1	powders					698:704	both camel and bovine whey powders	671:704	both camel and bovine whey powders	671:704	The α-Lactalbumin was relatively heat stable after drying and predominated the powder surface (X-ray photoelectron spectroscopy results) in both camel and bovine whey powders regardless the pH (neutral (6.7) or acidic (4.3 and 4.6)).
32683259	5	17	from	0.23	1069:1072	arg1	camel					1030:1034	camel	1030:1034	camel	1030:1034	Finally, decreasing the pH led to the decrease of the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity).
32683259	5	17	from	0.23	1069:1072	arg1	temperature					1015:1025	the glass transition temperature	994:1025	the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity)	994:1101	Finally, decreasing the pH led to the decrease of the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity).
32683259	5	17	from	0.23	1069:1072	arg1	powder					1052:1057	bovine whey powder	1040:1057	bovine whey powder (at 0.13, 0.23, and 0.33 of water activity)	1040:1101	Finally, decreasing the pH led to the decrease of the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity).
32683259	4	18	theme	water	867:871	arg1	activity					873:880	the water activity	863:880	the water activity of lactose crystallization for camel and bovine whey powders	863:941	Analysis of the water sorption isotherms indicated that decreasing the pH induced the increase of the water activity of lactose crystallization for camel and bovine whey powders.
32683259	1	19	theme	powders	336:342	arg1	temperature					289:299	the glass transition temperature	268:299	the glass transition temperature	268:299	This study investigated the effect of pH on the denaturation extent, the surface chemical composition, the water sorption isotherm and the glass transition temperature of camel and bovine whey protein's powders.
32683259	1	19	theme	powders	336:342	arg1	isotherm					255:262	the water sorption isotherm	236:262	the water sorption isotherm	236:262	This study investigated the effect of pH on the denaturation extent, the surface chemical composition, the water sorption isotherm and the glass transition temperature of camel and bovine whey protein's powders.
32683259	1	19	theme	powders	336:342	arg1	composition					223:233	the surface chemical composition	202:233	the surface chemical composition	202:233	This study investigated the effect of pH on the denaturation extent, the surface chemical composition, the water sorption isotherm and the glass transition temperature of camel and bovine whey protein's powders.
32683259	1	19	theme	powders	336:342	arg1	extent					194:199	the denaturation extent	177:199	the denaturation extent	177:199	This study investigated the effect of pH on the denaturation extent, the surface chemical composition, the water sorption isotherm and the glass transition temperature of camel and bovine whey protein's powders.
32683259	5	20	from	temperature	1015:1025	arg1	activity					1093:1100	water activity	1087:1100	water activity	1087:1100	Finally, decreasing the pH led to the decrease of the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity).
32683259	5	20	from	temperature	1015:1025	arg1	0.23					1069:1072	0.23	1069:1072	0.23	1069:1072	Finally, decreasing the pH led to the decrease of the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity).
32683259	5	20	from	temperature	1015:1025	arg1	0.33					1079:1082	0.33	1079:1082	0.33	1079:1082	Finally, decreasing the pH led to the decrease of the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity).
32683259	5	20	from	temperature	1015:1025	arg1	0.13					1063:1066	0.13	1063:1066	0.13	1063:1066	Finally, decreasing the pH led to the decrease of the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity).
32683259	4	21	theme	activity	873:880	arg1	increase					851:858	the increase	847:858	the increase of the water activity of lactose crystallization for camel and bovine whey powders	847:941	Analysis of the water sorption isotherms indicated that decreasing the pH induced the increase of the water activity of lactose crystallization for camel and bovine whey powders.
32683259	4	22	theme	crystallization	893:907	arg1	activity					873:880	the water activity	863:880	the water activity of lactose crystallization for camel and bovine whey powders	863:941	Analysis of the water sorption isotherms indicated that decreasing the pH induced the increase of the water activity of lactose crystallization for camel and bovine whey powders.
32683259	2	23	dep	protein	422:428	arg1	value					471:475	regardless the pH value	453:475	the most denatured protein in bovine whey powders regardless the pH value	403:475	The LC-MS analysis indicated that the β-Lactoglobulin was the most denatured protein in bovine whey powders regardless the pH value, while this protein was totally absent in camel whey.
32683259	4	24	theme	lactose	885:891	arg1	crystallization					893:907	lactose crystallization	885:907	lactose crystallization for camel and bovine whey powders	885:941	Analysis of the water sorption isotherms indicated that decreasing the pH induced the increase of the water activity of lactose crystallization for camel and bovine whey powders.
32683259	5	25	theme	glass	998:1002	arg1	temperature					1015:1025	the glass transition temperature	994:1025	the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity)	994:1101	Finally, decreasing the pH led to the decrease of the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity).
32683259	2	26	theme	LC-MS	349:353	arg1	analysis					355:362	The LC-MS analysis	345:362	The LC-MS analysis	345:362	The LC-MS analysis indicated that the β-Lactoglobulin was the most denatured protein in bovine whey powders regardless the pH value, while this protein was totally absent in camel whey.
32683259	2	27	theme	denatured	412:420	arg1	protein					422:428	the most denatured protein	403:428	the most denatured protein in bovine whey powders regardless the pH value	403:475	The LC-MS analysis indicated that the β-Lactoglobulin was the most denatured protein in bovine whey powders regardless the pH value, while this protein was totally absent in camel whey.
32683259	2	27	theme	denatured	412:420	arg1	β-Lactoglobulin					383:397	the β-Lactoglobulin	379:397	the β-Lactoglobulin	379:397	The LC-MS analysis indicated that the β-Lactoglobulin was the most denatured protein in bovine whey powders regardless the pH value, while this protein was totally absent in camel whey.
32683259	1	28	theme	sorption	246:253	arg1	isotherm					255:262	the water sorption isotherm	236:262	the water sorption isotherm	236:262	This study investigated the effect of pH on the denaturation extent, the surface chemical composition, the water sorption isotherm and the glass transition temperature of camel and bovine whey protein's powders.
32683259	4	29	theme	whey	930:933	arg1	powders					935:941	camel and bovine whey powders	913:941	camel and bovine whey powders	913:941	Analysis of the water sorption isotherms indicated that decreasing the pH induced the increase of the water activity of lactose crystallization for camel and bovine whey powders.
32683259	1	30	theme	pH	171:172	arg1	effect					161:166	the effect	157:166	the effect of pH on the denaturation extent, the surface chemical composition, the water sorption isotherm and the glass transition temperature of camel and bovine whey protein's powders	157:342	This study investigated the effect of pH on the denaturation extent, the surface chemical composition, the water sorption isotherm and the glass transition temperature of camel and bovine whey protein's powders.
32683259	0	31	theme	pH	10:11	arg1	Effect					0:5	Effect	0:5	Effect of pH on the physicochemical characteristics and the surface chemical composition of camel and bovine whey protein's powders.	0:131	Effect of pH on the physicochemical characteristics and the surface chemical composition of camel and bovine whey protein's powders.
32683259	4	32	theme	bovine	923:928	arg1	powders					935:941	camel and bovine whey powders	913:941	camel and bovine whey powders	913:941	Analysis of the water sorption isotherms indicated that decreasing the pH induced the increase of the water activity of lactose crystallization for camel and bovine whey powders.
32683259	0	33	theme	whey	109:112	arg1	protein					114:120	bovine whey protein	102:120	bovine whey protein	102:120	Effect of pH on the physicochemical characteristics and the surface chemical composition of camel and bovine whey protein's powders.
32683259	3	34	theme	powder	610:615	arg1	surface					617:623	the powder surface	606:623	the powder surface	606:623	The α-Lactalbumin was relatively heat stable after drying and predominated the powder surface (X-ray photoelectron spectroscopy results) in both camel and bovine whey powders regardless the pH (neutral (6.7) or acidic (4.3 and 4.6)).
32683259	5	35	theme	transition	1004:1013	arg1	temperature					1015:1025	the glass transition temperature	994:1025	the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity)	994:1101	Finally, decreasing the pH led to the decrease of the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity).
32683259	0	36	theme	physicochemical	20:34	arg1	characteristics					36:50	the physicochemical characteristics	16:50	the physicochemical characteristics	16:50	Effect of pH on the physicochemical characteristics and the surface chemical composition of camel and bovine whey protein's powders.
32683259	0	37	theme	powders	124:130	arg1	composition					77:87	the surface chemical composition	56:87	the surface chemical composition	56:87	Effect of pH on the physicochemical characteristics and the surface chemical composition of camel and bovine whey protein's powders.
32683259	0	37	theme	powders	124:130	arg1	characteristics					36:50	the physicochemical characteristics	16:50	the physicochemical characteristics	16:50	Effect of pH on the physicochemical characteristics and the surface chemical composition of camel and bovine whey protein's powders.
32683259	1	38	theme	denaturation	181:192	arg1	extent					194:199	the denaturation extent	177:199	the denaturation extent	177:199	This study investigated the effect of pH on the denaturation extent, the surface chemical composition, the water sorption isotherm and the glass transition temperature of camel and bovine whey protein's powders.
32683259	2	39	theme	whey	440:443	arg1	powders					445:451	bovine whey powders	433:451	bovine whey powders	433:451	The LC-MS analysis indicated that the β-Lactoglobulin was the most denatured protein in bovine whey powders regardless the pH value, while this protein was totally absent in camel whey.
32683259	1	40	theme	glass	272:276	arg1	transition					278:287	the glass transition	268:287	the glass transition temperature	268:299	This study investigated the effect of pH on the denaturation extent, the surface chemical composition, the water sorption isotherm and the glass transition temperature of camel and bovine whey protein's powders.
32683259	4	41	theme	camel	913:917	arg1	powders					935:941	camel and bovine whey powders	913:941	camel and bovine whey powders	913:941	Analysis of the water sorption isotherms indicated that decreasing the pH induced the increase of the water activity of lactose crystallization for camel and bovine whey powders.
32683259	2	42	theme	bovine	433:438	arg1	powders					445:451	bovine whey powders	433:451	bovine whey powders	433:451	The LC-MS analysis indicated that the β-Lactoglobulin was the most denatured protein in bovine whey powders regardless the pH value, while this protein was totally absent in camel whey.
32683259	1	43	theme	transition	278:287	arg1	temperature					289:299	the glass transition temperature	268:299	the glass transition temperature	268:299	This study investigated the effect of pH on the denaturation extent, the surface chemical composition, the water sorption isotherm and the glass transition temperature of camel and bovine whey protein's powders.
32683259	3	44	theme	camel	676:680	arg1	powders					698:704	both camel and bovine whey powders	671:704	both camel and bovine whey powders	671:704	The α-Lactalbumin was relatively heat stable after drying and predominated the powder surface (X-ray photoelectron spectroscopy results) in both camel and bovine whey powders regardless the pH (neutral (6.7) or acidic (4.3 and 4.6)).
32683259	1	45	theme	whey	321:324	arg1	protein					326:332	bovine whey protein	314:332	bovine whey protein	314:332	This study investigated the effect of pH on the denaturation extent, the surface chemical composition, the water sorption isotherm and the glass transition temperature of camel and bovine whey protein's powders.
32683259	5	46	theme	temperature	1015:1025	arg1	decrease					982:989	the decrease	978:989	the decrease of the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity)	978:1101	Finally, decreasing the pH led to the decrease of the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity).
32683259	3	47	theme	photoelectron	632:644	arg1	results					659:665	X-ray photoelectron spectroscopy results	626:665	X-ray photoelectron spectroscopy results	626:665	The α-Lactalbumin was relatively heat stable after drying and predominated the powder surface (X-ray photoelectron spectroscopy results) in both camel and bovine whey powders regardless the pH (neutral (6.7) or acidic (4.3 and 4.6)).
32683259	5	48	theme	whey	1047:1050	arg1	powder					1052:1057	bovine whey powder	1040:1057	bovine whey powder (at 0.13, 0.23, and 0.33 of water activity)	1040:1101	Finally, decreasing the pH led to the decrease of the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity).
32683259	2	49	from	protein	422:428	arg1	powders					445:451	bovine whey powders	433:451	bovine whey powders	433:451	The LC-MS analysis indicated that the β-Lactoglobulin was the most denatured protein in bovine whey powders regardless the pH value, while this protein was totally absent in camel whey.
32683259	3	50	theme	spectroscopy	646:657	arg1	results					659:665	X-ray photoelectron spectroscopy results	626:665	X-ray photoelectron spectroscopy results	626:665	The α-Lactalbumin was relatively heat stable after drying and predominated the powder surface (X-ray photoelectron spectroscopy results) in both camel and bovine whey powders regardless the pH (neutral (6.7) or acidic (4.3 and 4.6)).
32683259	3	51	dep	predominated	593:604	arg1	results					659:665	X-ray photoelectron spectroscopy results	626:665	X-ray photoelectron spectroscopy results	626:665	The α-Lactalbumin was relatively heat stable after drying and predominated the powder surface (X-ray photoelectron spectroscopy results) in both camel and bovine whey powders regardless the pH (neutral (6.7) or acidic (4.3 and 4.6)).
32683259	2	52	theme	camel	519:523	arg1	whey					525:528	camel whey	519:528	camel whey	519:528	The LC-MS analysis indicated that the β-Lactoglobulin was the most denatured protein in bovine whey powders regardless the pH value, while this protein was totally absent in camel whey.
32683259	0	53	theme	surface	60:66	arg1	composition					77:87	the surface chemical composition	56:87	the surface chemical composition	56:87	Effect of pH on the physicochemical characteristics and the surface chemical composition of camel and bovine whey protein's powders.
32683259	3	54	theme	X-ray	626:630	arg1	results					659:665	X-ray photoelectron spectroscopy results	626:665	X-ray photoelectron spectroscopy results	626:665	The α-Lactalbumin was relatively heat stable after drying and predominated the powder surface (X-ray photoelectron spectroscopy results) in both camel and bovine whey powders regardless the pH (neutral (6.7) or acidic (4.3 and 4.6)).
32683259	5	55	theme	bovine	1040:1045	arg1	powder					1052:1057	bovine whey powder	1040:1057	bovine whey powder (at 0.13, 0.23, and 0.33 of water activity)	1040:1101	Finally, decreasing the pH led to the decrease of the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity).
32683259	1	56	theme	surface	206:212	arg1	composition					223:233	the surface chemical composition	202:233	the surface chemical composition	202:233	This study investigated the effect of pH on the denaturation extent, the surface chemical composition, the water sorption isotherm and the glass transition temperature of camel and bovine whey protein's powders.
32683259	2	57	theme	pH	468:469	arg1	value					471:475	regardless the pH value	453:475	the most denatured protein in bovine whey powders regardless the pH value	403:475	The LC-MS analysis indicated that the β-Lactoglobulin was the most denatured protein in bovine whey powders regardless the pH value, while this protein was totally absent in camel whey.
32683259	5	58	theme	powder	1052:1057	arg1	temperature					1015:1025	the glass transition temperature	994:1025	the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity)	994:1101	Finally, decreasing the pH led to the decrease of the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity).
32683259	2	59	from	absent	509:514	arg1	whey					525:528	camel whey	519:528	camel whey	519:528	The LC-MS analysis indicated that the β-Lactoglobulin was the most denatured protein in bovine whey powders regardless the pH value, while this protein was totally absent in camel whey.
32683259	5	60	theme	activity	1093:1100	arg1	activity					1093:1100	water activity	1087:1100	water activity	1087:1100	Finally, decreasing the pH led to the decrease of the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity).
32683259	5	60	theme	activity	1093:1100	arg1	0.13					1063:1066	0.13	1063:1066	0.13	1063:1066	Finally, decreasing the pH led to the decrease of the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity).
32683259	5	61	from	0.33	1079:1082	arg1	camel					1030:1034	camel	1030:1034	camel	1030:1034	Finally, decreasing the pH led to the decrease of the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity).
32683259	5	61	from	0.33	1079:1082	arg1	temperature					1015:1025	the glass transition temperature	994:1025	the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity)	994:1101	Finally, decreasing the pH led to the decrease of the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity).
32683259	5	61	from	0.33	1079:1082	arg1	powder					1052:1057	bovine whey powder	1040:1057	bovine whey powder (at 0.13, 0.23, and 0.33 of water activity)	1040:1101	Finally, decreasing the pH led to the decrease of the glass transition temperature of camel and bovine whey powder (at 0.13, 0.23, and 0.33 of water activity).
32683259	1	62	theme	chemical	214:221	arg1	composition					223:233	the surface chemical composition	202:233	the surface chemical composition	202:233	This study investigated the effect of pH on the denaturation extent, the surface chemical composition, the water sorption isotherm and the glass transition temperature of camel and bovine whey protein's powders.
32436815	0	0	theme	Mg-Zn-Y-Nd	75:84	arg1	alloy					86:90	the silanic Mg-Zn-Y-Nd alloy	63:90	the silanic Mg-Zn-Y-Nd alloy for better endothelialization	63:120	Conjugating heparin, Arg-Glu-Asp-Val peptide, and anti-CD34 to the silanic Mg-Zn-Y-Nd alloy for better endothelialization.
32436815	4	1	theme	anti-coagulation	1012:1027	arg1	consideration					995:1007	the consideration	991:1007	the consideration of anti-coagulation and anti-inflammation	991:1049	In addition, the heparin was also immobilized to the Mg-Zn-Y-Nd surface for the consideration of anti-coagulation and anti-inflammation.
32436815	5	2	theme	Mg-Zn-Y-Nd	1182:1191	arg1	alloy					1193:1197	Mg-Zn-Y-Nd alloy	1182:1197	Mg-Zn-Y-Nd alloy	1182:1197	Systematic material characterization and biological evaluation show that we have successfully developed this composite surface on Mg-Zn-Y-Nd alloy, and achieved multiple functions such as corrosion resistance, promoting endothelialization, and inhibiting platelet/macrophage adhesion.
32436815	5	3	theme	composite	1161:1169	arg1	surface					1171:1177	this composite surface	1156:1177	this composite surface on Mg-Zn-Y-Nd alloy	1156:1197	Systematic material characterization and biological evaluation show that we have successfully developed this composite surface on Mg-Zn-Y-Nd alloy, and achieved multiple functions such as corrosion resistance, promoting endothelialization, and inhibiting platelet/macrophage adhesion.
32436815	3	4	theme	progenitor	838:847	arg1	cells					849:853	endothelial progenitor cells	826:853	endothelial progenitor cells	826:853	In this study, we selected Mg-Zn-Y-Nd, a kind of biodegradable magnesium alloy for cardiovascular stent, and passivated its surface by alkali heat treatment and silane treatment to improve the corrosion resistance, subsequently conjugated Arg-Glu-Asp-Val (REDV) peptide and anti-CD34 to promote endothelial cells adhesion and capture endothelial progenitor cells respectively, further improving surface endothelialization.
32436815	2	5	theme	stent	485:489	arg1	application					454:464	further application	446:464	further application of magnesium alloy stent	446:489	However, too fast degradation rate and delayed surface endothelialization have been the bottleneck of further application of magnesium alloy stent.
32436815	1	6	from	hotspots	320:327	arg1	field					337:341	this field	332:341	this field	332:341	Magnesium alloy is generally accepted as a potential cardiovascular stent material due to its good mechanical properties, biocompatibility, and biodegradability, and has become one of the research hotspots in this field.
32436815	3	7	theme	surface	887:893	arg1	endothelialization					895:912	surface endothelialization	887:912	surface endothelialization	887:912	In this study, we selected Mg-Zn-Y-Nd, a kind of biodegradable magnesium alloy for cardiovascular stent, and passivated its surface by alkali heat treatment and silane treatment to improve the corrosion resistance, subsequently conjugated Arg-Glu-Asp-Val (REDV) peptide and anti-CD34 to promote endothelial cells adhesion and capture endothelial progenitor cells respectively, further improving surface endothelialization.
32436815	4	8	theme	Mg-Zn-Y-Nd	968:977	arg1	surface					979:985	the Mg-Zn-Y-Nd surface	964:985	the Mg-Zn-Y-Nd surface	964:985	In addition, the heparin was also immobilized to the Mg-Zn-Y-Nd surface for the consideration of anti-coagulation and anti-inflammation.
32436815	3	9	theme	conjugated	720:729	arg1	peptide					754:760	subsequently conjugated Arg-Glu-Asp-Val (REDV) peptide	707:760	subsequently conjugated Arg-Glu-Asp-Val (REDV) peptide	707:760	In this study, we selected Mg-Zn-Y-Nd, a kind of biodegradable magnesium alloy for cardiovascular stent, and passivated its surface by alkali heat treatment and silane treatment to improve the corrosion resistance, subsequently conjugated Arg-Glu-Asp-Val (REDV) peptide and anti-CD34 to promote endothelial cells adhesion and capture endothelial progenitor cells respectively, further improving surface endothelialization.
32436815	3	10	theme	endothelial	826:836	arg1	cells					849:853	endothelial progenitor cells	826:853	endothelial progenitor cells	826:853	In this study, we selected Mg-Zn-Y-Nd, a kind of biodegradable magnesium alloy for cardiovascular stent, and passivated its surface by alkali heat treatment and silane treatment to improve the corrosion resistance, subsequently conjugated Arg-Glu-Asp-Val (REDV) peptide and anti-CD34 to promote endothelial cells adhesion and capture endothelial progenitor cells respectively, further improving surface endothelialization.
32436815	3	11	theme	Arg-Glu-Asp-Val	731:745	arg1	peptide					754:760	subsequently conjugated Arg-Glu-Asp-Val (REDV) peptide	707:760	subsequently conjugated Arg-Glu-Asp-Val (REDV) peptide	707:760	In this study, we selected Mg-Zn-Y-Nd, a kind of biodegradable magnesium alloy for cardiovascular stent, and passivated its surface by alkali heat treatment and silane treatment to improve the corrosion resistance, subsequently conjugated Arg-Glu-Asp-Val (REDV) peptide and anti-CD34 to promote endothelial cells adhesion and capture endothelial progenitor cells respectively, further improving surface endothelialization.
32436815	1	12	theme	potential	166:174	arg1	alloy					133:137	Magnesium alloy	123:137	Magnesium alloy	123:137	Magnesium alloy is generally accepted as a potential cardiovascular stent material due to its good mechanical properties, biocompatibility, and biodegradability, and has become one of the research hotspots in this field.
32436815	1	12	theme	potential	166:174	arg1	material					197:204	a potential cardiovascular stent material	164:204	a potential cardiovascular stent material due to its good mechanical properties, biocompatibility, and biodegradability	164:282	Magnesium alloy is generally accepted as a potential cardiovascular stent material due to its good mechanical properties, biocompatibility, and biodegradability, and has become one of the research hotspots in this field.
32436815	0	13	theme	better	96:101	arg1	endothelialization					103:120	better endothelialization	96:120	better endothelialization	96:120	Conjugating heparin, Arg-Glu-Asp-Val peptide, and anti-CD34 to the silanic Mg-Zn-Y-Nd alloy for better endothelialization.
32436815	4	14	theme	anti-inflammation	1033:1049	arg1	consideration					995:1007	the consideration	991:1007	the consideration of anti-coagulation and anti-inflammation	991:1049	In addition, the heparin was also immobilized to the Mg-Zn-Y-Nd surface for the consideration of anti-coagulation and anti-inflammation.
32436815	1	15	theme	cardiovascular	176:189	arg1	alloy					133:137	Magnesium alloy	123:137	Magnesium alloy	123:137	Magnesium alloy is generally accepted as a potential cardiovascular stent material due to its good mechanical properties, biocompatibility, and biodegradability, and has become one of the research hotspots in this field.
32436815	1	15	theme	cardiovascular	176:189	arg1	material					197:204	a potential cardiovascular stent material	164:204	a potential cardiovascular stent material due to its good mechanical properties, biocompatibility, and biodegradability	164:282	Magnesium alloy is generally accepted as a potential cardiovascular stent material due to its good mechanical properties, biocompatibility, and biodegradability, and has become one of the research hotspots in this field.
32436815	2	16	theme	further	446:452	arg1	application					454:464	further application	446:464	further application of magnesium alloy stent	446:489	However, too fast degradation rate and delayed surface endothelialization have been the bottleneck of further application of magnesium alloy stent.
32436815	0	17	theme	silanic	67:73	arg1	alloy					86:90	the silanic Mg-Zn-Y-Nd alloy	63:90	the silanic Mg-Zn-Y-Nd alloy for better endothelialization	63:120	Conjugating heparin, Arg-Glu-Asp-Val peptide, and anti-CD34 to the silanic Mg-Zn-Y-Nd alloy for better endothelialization.
32436815	3	18	theme	magnesium	555:563	arg1	alloy					565:569	biodegradable magnesium alloy	541:569	biodegradable magnesium alloy for cardiovascular stent	541:594	In this study, we selected Mg-Zn-Y-Nd, a kind of biodegradable magnesium alloy for cardiovascular stent, and passivated its surface by alkali heat treatment and silane treatment to improve the corrosion resistance, subsequently conjugated Arg-Glu-Asp-Val (REDV) peptide and anti-CD34 to promote endothelial cells adhesion and capture endothelial progenitor cells respectively, further improving surface endothelialization.
32436815	1	19	theme	stent	191:195	arg1	alloy					133:137	Magnesium alloy	123:137	Magnesium alloy	123:137	Magnesium alloy is generally accepted as a potential cardiovascular stent material due to its good mechanical properties, biocompatibility, and biodegradability, and has become one of the research hotspots in this field.
32436815	1	19	theme	stent	191:195	arg1	material					197:204	a potential cardiovascular stent material	164:204	a potential cardiovascular stent material due to its good mechanical properties, biocompatibility, and biodegradability	164:282	Magnesium alloy is generally accepted as a potential cardiovascular stent material due to its good mechanical properties, biocompatibility, and biodegradability, and has become one of the research hotspots in this field.
32436815	5	20	theme	corrosion	1240:1248	arg1	resistance					1250:1259	corrosion resistance	1240:1259	corrosion resistance	1240:1259	Systematic material characterization and biological evaluation show that we have successfully developed this composite surface on Mg-Zn-Y-Nd alloy, and achieved multiple functions such as corrosion resistance, promoting endothelialization, and inhibiting platelet/macrophage adhesion.
32436815	3	21	theme	silane	653:658	arg1	treatment					660:668	silane treatment	653:668	silane treatment	653:668	In this study, we selected Mg-Zn-Y-Nd, a kind of biodegradable magnesium alloy for cardiovascular stent, and passivated its surface by alkali heat treatment and silane treatment to improve the corrosion resistance, subsequently conjugated Arg-Glu-Asp-Val (REDV) peptide and anti-CD34 to promote endothelial cells adhesion and capture endothelial progenitor cells respectively, further improving surface endothelialization.
32436815	3	22	theme	alloy	565:569	arg1	Mg-Zn-Y-Nd					519:528	Mg-Zn-Y-Nd	519:528	Mg-Zn-Y-Nd	519:528	In this study, we selected Mg-Zn-Y-Nd, a kind of biodegradable magnesium alloy for cardiovascular stent, and passivated its surface by alkali heat treatment and silane treatment to improve the corrosion resistance, subsequently conjugated Arg-Glu-Asp-Val (REDV) peptide and anti-CD34 to promote endothelial cells adhesion and capture endothelial progenitor cells respectively, further improving surface endothelialization.
32436815	3	22	theme	alloy	565:569	arg1	kind					533:536	a kind	531:536	a kind of biodegradable magnesium alloy for cardiovascular stent	531:594	In this study, we selected Mg-Zn-Y-Nd, a kind of biodegradable magnesium alloy for cardiovascular stent, and passivated its surface by alkali heat treatment and silane treatment to improve the corrosion resistance, subsequently conjugated Arg-Glu-Asp-Val (REDV) peptide and anti-CD34 to promote endothelial cells adhesion and capture endothelial progenitor cells respectively, further improving surface endothelialization.
32436815	2	23	theme	delayed	383:389	arg1	endothelialization					399:416	delayed surface endothelialization	383:416	delayed surface endothelialization	383:416	However, too fast degradation rate and delayed surface endothelialization have been the bottleneck of further application of magnesium alloy stent.
32436815	2	23	theme	delayed	383:389	arg1	bottleneck					432:441	the bottleneck	428:441	the bottleneck of further application of magnesium alloy stent	428:489	However, too fast degradation rate and delayed surface endothelialization have been the bottleneck of further application of magnesium alloy stent.
32436815	2	23	theme	delayed	383:389	arg1	rate					374:377	too fast degradation rate	353:377	too fast degradation rate	353:377	However, too fast degradation rate and delayed surface endothelialization have been the bottleneck of further application of magnesium alloy stent.
32436815	2	24	theme	degradation	362:372	arg1	endothelialization					399:416	delayed surface endothelialization	383:416	delayed surface endothelialization	383:416	However, too fast degradation rate and delayed surface endothelialization have been the bottleneck of further application of magnesium alloy stent.
32436815	2	24	theme	degradation	362:372	arg1	rate					374:377	too fast degradation rate	353:377	too fast degradation rate	353:377	However, too fast degradation rate and delayed surface endothelialization have been the bottleneck of further application of magnesium alloy stent.
32436815	2	24	theme	degradation	362:372	arg1	bottleneck					432:441	the bottleneck	428:441	the bottleneck of further application of magnesium alloy stent	428:489	However, too fast degradation rate and delayed surface endothelialization have been the bottleneck of further application of magnesium alloy stent.
32436815	0	25	theme	Arg-Glu-Asp-Val	21:35	arg1	peptide					37:43	Arg-Glu-Asp-Val peptide	21:43	Arg-Glu-Asp-Val peptide	21:43	Conjugating heparin, Arg-Glu-Asp-Val peptide, and anti-CD34 to the silanic Mg-Zn-Y-Nd alloy for better endothelialization.
32436815	5	26	theme	biological	1093:1102	arg1	evaluation					1104:1113	biological evaluation	1093:1113	biological evaluation	1093:1113	Systematic material characterization and biological evaluation show that we have successfully developed this composite surface on Mg-Zn-Y-Nd alloy, and achieved multiple functions such as corrosion resistance, promoting endothelialization, and inhibiting platelet/macrophage adhesion.
32436815	1	27	theme	due	206:208	arg1	alloy					133:137	Magnesium alloy	123:137	Magnesium alloy	123:137	Magnesium alloy is generally accepted as a potential cardiovascular stent material due to its good mechanical properties, biocompatibility, and biodegradability, and has become one of the research hotspots in this field.
32436815	1	27	theme	due	206:208	arg1	material					197:204	a potential cardiovascular stent material	164:204	a potential cardiovascular stent material due to its good mechanical properties, biocompatibility, and biodegradability	164:282	Magnesium alloy is generally accepted as a potential cardiovascular stent material due to its good mechanical properties, biocompatibility, and biodegradability, and has become one of the research hotspots in this field.
32436815	5	28	from	surface	1171:1177	arg1	alloy					1193:1197	Mg-Zn-Y-Nd alloy	1182:1197	Mg-Zn-Y-Nd alloy	1182:1197	Systematic material characterization and biological evaluation show that we have successfully developed this composite surface on Mg-Zn-Y-Nd alloy, and achieved multiple functions such as corrosion resistance, promoting endothelialization, and inhibiting platelet/macrophage adhesion.
32436815	3	29	theme	heat	634:637	arg1	treatment					639:647	alkali heat treatment	627:647	alkali heat treatment	627:647	In this study, we selected Mg-Zn-Y-Nd, a kind of biodegradable magnesium alloy for cardiovascular stent, and passivated its surface by alkali heat treatment and silane treatment to improve the corrosion resistance, subsequently conjugated Arg-Glu-Asp-Val (REDV) peptide and anti-CD34 to promote endothelial cells adhesion and capture endothelial progenitor cells respectively, further improving surface endothelialization.
32436815	3	30	theme	biodegradable	541:553	arg1	alloy					565:569	biodegradable magnesium alloy	541:569	biodegradable magnesium alloy for cardiovascular stent	541:594	In this study, we selected Mg-Zn-Y-Nd, a kind of biodegradable magnesium alloy for cardiovascular stent, and passivated its surface by alkali heat treatment and silane treatment to improve the corrosion resistance, subsequently conjugated Arg-Glu-Asp-Val (REDV) peptide and anti-CD34 to promote endothelial cells adhesion and capture endothelial progenitor cells respectively, further improving surface endothelialization.
32436815	2	31	theme	alloy	479:483	arg1	stent					485:489	magnesium alloy stent	469:489	magnesium alloy stent	469:489	However, too fast degradation rate and delayed surface endothelialization have been the bottleneck of further application of magnesium alloy stent.
32436815	3	32	theme	REDV	748:751	arg1	peptide					754:760	subsequently conjugated Arg-Glu-Asp-Val (REDV) peptide	707:760	subsequently conjugated Arg-Glu-Asp-Val (REDV) peptide	707:760	In this study, we selected Mg-Zn-Y-Nd, a kind of biodegradable magnesium alloy for cardiovascular stent, and passivated its surface by alkali heat treatment and silane treatment to improve the corrosion resistance, subsequently conjugated Arg-Glu-Asp-Val (REDV) peptide and anti-CD34 to promote endothelial cells adhesion and capture endothelial progenitor cells respectively, further improving surface endothelialization.
32436815	5	33	theme	material	1063:1070	arg1	characterization					1072:1087	Systematic material characterization	1052:1087	Systematic material characterization	1052:1087	Systematic material characterization and biological evaluation show that we have successfully developed this composite surface on Mg-Zn-Y-Nd alloy, and achieved multiple functions such as corrosion resistance, promoting endothelialization, and inhibiting platelet/macrophage adhesion.
32436815	2	34	theme	magnesium	469:477	arg1	stent					485:489	magnesium alloy stent	469:489	magnesium alloy stent	469:489	However, too fast degradation rate and delayed surface endothelialization have been the bottleneck of further application of magnesium alloy stent.
32436815	5	35	theme	multiple	1213:1220	arg1	resistance					1250:1259	corrosion resistance	1240:1259	corrosion resistance	1240:1259	Systematic material characterization and biological evaluation show that we have successfully developed this composite surface on Mg-Zn-Y-Nd alloy, and achieved multiple functions such as corrosion resistance, promoting endothelialization, and inhibiting platelet/macrophage adhesion.
32436815	5	35	theme	multiple	1213:1220	arg1	functions					1222:1230	multiple functions	1213:1230	multiple functions such as corrosion resistance	1213:1259	Systematic material characterization and biological evaluation show that we have successfully developed this composite surface on Mg-Zn-Y-Nd alloy, and achieved multiple functions such as corrosion resistance, promoting endothelialization, and inhibiting platelet/macrophage adhesion.
32436815	1	36	theme	good	217:220	arg1	properties					233:242	its good mechanical properties	213:242	its good mechanical properties	213:242	Magnesium alloy is generally accepted as a potential cardiovascular stent material due to its good mechanical properties, biocompatibility, and biodegradability, and has become one of the research hotspots in this field.
32436815	3	37	theme	alkali	627:632	arg1	treatment					639:647	alkali heat treatment	627:647	alkali heat treatment	627:647	In this study, we selected Mg-Zn-Y-Nd, a kind of biodegradable magnesium alloy for cardiovascular stent, and passivated its surface by alkali heat treatment and silane treatment to improve the corrosion resistance, subsequently conjugated Arg-Glu-Asp-Val (REDV) peptide and anti-CD34 to promote endothelial cells adhesion and capture endothelial progenitor cells respectively, further improving surface endothelialization.
32436815	2	38	theme	application	454:464	arg1	endothelialization					399:416	delayed surface endothelialization	383:416	delayed surface endothelialization	383:416	However, too fast degradation rate and delayed surface endothelialization have been the bottleneck of further application of magnesium alloy stent.
32436815	2	38	theme	application	454:464	arg1	bottleneck					432:441	the bottleneck	428:441	the bottleneck of further application of magnesium alloy stent	428:489	However, too fast degradation rate and delayed surface endothelialization have been the bottleneck of further application of magnesium alloy stent.
32436815	2	38	theme	application	454:464	arg1	rate					374:377	too fast degradation rate	353:377	too fast degradation rate	353:377	However, too fast degradation rate and delayed surface endothelialization have been the bottleneck of further application of magnesium alloy stent.
32436815	1	39	theme	research	311:318	arg1	hotspots					320:327	the research hotspots	307:327	the research hotspots in this field	307:341	Magnesium alloy is generally accepted as a potential cardiovascular stent material due to its good mechanical properties, biocompatibility, and biodegradability, and has become one of the research hotspots in this field.
32436815	3	40	theme	cardiovascular	575:588	arg1	stent					590:594	cardiovascular stent	575:594	cardiovascular stent	575:594	In this study, we selected Mg-Zn-Y-Nd, a kind of biodegradable magnesium alloy for cardiovascular stent, and passivated its surface by alkali heat treatment and silane treatment to improve the corrosion resistance, subsequently conjugated Arg-Glu-Asp-Val (REDV) peptide and anti-CD34 to promote endothelial cells adhesion and capture endothelial progenitor cells respectively, further improving surface endothelialization.
32436815	1	41	theme	Magnesium	123:131	arg1	alloy					133:137	Magnesium alloy	123:137	Magnesium alloy	123:137	Magnesium alloy is generally accepted as a potential cardiovascular stent material due to its good mechanical properties, biocompatibility, and biodegradability, and has become one of the research hotspots in this field.
32436815	1	41	theme	Magnesium	123:131	arg1	material					197:204	a potential cardiovascular stent material	164:204	a potential cardiovascular stent material due to its good mechanical properties, biocompatibility, and biodegradability	164:282	Magnesium alloy is generally accepted as a potential cardiovascular stent material due to its good mechanical properties, biocompatibility, and biodegradability, and has become one of the research hotspots in this field.
32436815	1	42	theme	hotspots	320:327	arg1	one					300:302	one	300:302	one	300:302	Magnesium alloy is generally accepted as a potential cardiovascular stent material due to its good mechanical properties, biocompatibility, and biodegradability, and has become one of the research hotspots in this field.
32436815	1	42	theme	hotspots	320:327	arg1	hotspots					320:327	the research hotspots	307:327	the research hotspots in this field	307:341	Magnesium alloy is generally accepted as a potential cardiovascular stent material due to its good mechanical properties, biocompatibility, and biodegradability, and has become one of the research hotspots in this field.
32436815	1	43	theme	mechanical	222:231	arg1	properties					233:242	its good mechanical properties	213:242	its good mechanical properties	213:242	Magnesium alloy is generally accepted as a potential cardiovascular stent material due to its good mechanical properties, biocompatibility, and biodegradability, and has become one of the research hotspots in this field.
32436815	2	44	theme	surface	391:397	arg1	endothelialization					399:416	delayed surface endothelialization	383:416	delayed surface endothelialization	383:416	However, too fast degradation rate and delayed surface endothelialization have been the bottleneck of further application of magnesium alloy stent.
32436815	2	44	theme	surface	391:397	arg1	bottleneck					432:441	the bottleneck	428:441	the bottleneck of further application of magnesium alloy stent	428:489	However, too fast degradation rate and delayed surface endothelialization have been the bottleneck of further application of magnesium alloy stent.
32436815	2	44	theme	surface	391:397	arg1	rate					374:377	too fast degradation rate	353:377	too fast degradation rate	353:377	However, too fast degradation rate and delayed surface endothelialization have been the bottleneck of further application of magnesium alloy stent.
32436815	2	45	theme	fast	357:360	arg1	endothelialization					399:416	delayed surface endothelialization	383:416	delayed surface endothelialization	383:416	However, too fast degradation rate and delayed surface endothelialization have been the bottleneck of further application of magnesium alloy stent.
32436815	2	45	theme	fast	357:360	arg1	rate					374:377	too fast degradation rate	353:377	too fast degradation rate	353:377	However, too fast degradation rate and delayed surface endothelialization have been the bottleneck of further application of magnesium alloy stent.
32436815	2	45	theme	fast	357:360	arg1	bottleneck					432:441	the bottleneck	428:441	the bottleneck of further application of magnesium alloy stent	428:489	However, too fast degradation rate and delayed surface endothelialization have been the bottleneck of further application of magnesium alloy stent.
32436815	3	46	theme	endothelial	787:797	arg1	adhesion					805:812	endothelial cells adhesion	787:812	endothelial cells adhesion	787:812	In this study, we selected Mg-Zn-Y-Nd, a kind of biodegradable magnesium alloy for cardiovascular stent, and passivated its surface by alkali heat treatment and silane treatment to improve the corrosion resistance, subsequently conjugated Arg-Glu-Asp-Val (REDV) peptide and anti-CD34 to promote endothelial cells adhesion and capture endothelial progenitor cells respectively, further improving surface endothelialization.
32436815	3	47	theme	corrosion	685:693	arg1	resistance					695:704	the corrosion resistance	681:704	the corrosion resistance	681:704	In this study, we selected Mg-Zn-Y-Nd, a kind of biodegradable magnesium alloy for cardiovascular stent, and passivated its surface by alkali heat treatment and silane treatment to improve the corrosion resistance, subsequently conjugated Arg-Glu-Asp-Val (REDV) peptide and anti-CD34 to promote endothelial cells adhesion and capture endothelial progenitor cells respectively, further improving surface endothelialization.
32436815	5	48	theme	Systematic	1052:1061	arg1	characterization					1072:1087	Systematic material characterization	1052:1087	Systematic material characterization	1052:1087	Systematic material characterization and biological evaluation show that we have successfully developed this composite surface on Mg-Zn-Y-Nd alloy, and achieved multiple functions such as corrosion resistance, promoting endothelialization, and inhibiting platelet/macrophage adhesion.
32436815	5	49	theme	platelet/macrophage	1307:1325	arg1	adhesion					1327:1334	platelet/macrophage adhesion	1307:1334	platelet/macrophage adhesion	1307:1334	Systematic material characterization and biological evaluation show that we have successfully developed this composite surface on Mg-Zn-Y-Nd alloy, and achieved multiple functions such as corrosion resistance, promoting endothelialization, and inhibiting platelet/macrophage adhesion.
32436815	3	50	theme	cells	799:803	arg1	adhesion					805:812	endothelial cells adhesion	787:812	endothelial cells adhesion	787:812	In this study, we selected Mg-Zn-Y-Nd, a kind of biodegradable magnesium alloy for cardiovascular stent, and passivated its surface by alkali heat treatment and silane treatment to improve the corrosion resistance, subsequently conjugated Arg-Glu-Asp-Val (REDV) peptide and anti-CD34 to promote endothelial cells adhesion and capture endothelial progenitor cells respectively, further improving surface endothelialization.
32135252	0	0	theme	levodopa	105:112	arg1	release					94:100	prolonged release	84:100	prolonged release of levodopa	84:112	Multi-stimuli responsive nanogel/hydrogel nanocomposites based on κ-carrageenan for prolonged release of levodopa as model drug.
32135252	6	1	theme	prolonged	1089:1097	arg1	carrier					1114:1120	a prolonged releasing drug carrier	1087:1120	a prolonged releasing drug carrier for levodopa (L-DOPA)	1087:1142	This biopolymer based nanogel/hydrogel used as a prolonged releasing drug carrier for levodopa (L-DOPA) as a main drug for treating Parkinson's symptoms.
32135252	3	2	theme	poly	545:548	arg1	nanoparticles					642:654	poly (acrylic acid) grafted onto κ-carrageenan polysaccharide with entrapped magnetic iron oxide nanoparticles	545:654	poly (acrylic acid) grafted onto κ-carrageenan polysaccharide with entrapped magnetic iron oxide nanoparticles as crosslinker	545:669	The nanogels were introduced during the formation of a hydrogel network to produce nanogel/hydrogel soft nanocomposites, while the biocompatible hydrogel was based on poly (acrylic acid) grafted onto κ-carrageenan polysaccharide with entrapped magnetic iron oxide nanoparticles as crosslinker.
32135252	1	3	theme	novel	171:175	arg1	system					222:227	a novel biocompatible nanogel/hydrogel nanocomposite system	169:227	a novel biocompatible nanogel/hydrogel nanocomposite system for long-term delivery of bioactive molecules	169:273	This study describes the development of a novel biocompatible nanogel/hydrogel nanocomposite system for long-term delivery of bioactive molecules.
32135252	8	4	theme	pH-dependent	1260:1271	arg1	release					1273:1279	pH-dependent release	1260:1279	pH-dependent release of the active drug	1260:1298	pH-dependent release of the active drug can be sustained for >11 days from this soft nanocomposite.
32135252	9	5	theme	other	1436:1440	arg1	drugs					1442:1446	other drugs	1436:1446	other drugs	1436:1446	The results demonstrated a sustained release model that can be extended for other drugs.
32135252	0	6	theme	model	117:121	arg1	drug					123:126	model drug	117:126	model drug	117:126	Multi-stimuli responsive nanogel/hydrogel nanocomposites based on κ-carrageenan for prolonged release of levodopa as model drug.
32135252	7	7	dep	in	1198:1199	arg1	vitro					1201:1205	vitro	1201:1205	vitro	1201:1205	The in vitro release of L-DOPA was investigated at different pHs.
32135252	5	8	theme	scanning	1004:1011	arg1	SEM					1034:1036	SEM	1034:1036	SEM	1034:1036	The obtained soft nanocomposite was characterized by Fourier transform infrared spectrum (FT-IR), thermogravimetric (TG) analysis, and scanning electron microscopy (SEM).
32135252	5	8	theme	scanning	1004:1011	arg1	microscopy					1022:1031	scanning electron microscopy	1004:1031	scanning electron microscopy (SEM)	1004:1037	The obtained soft nanocomposite was characterized by Fourier transform infrared spectrum (FT-IR), thermogravimetric (TG) analysis, and scanning electron microscopy (SEM).
32135252	4	9	theme	functional	718:727	arg1	polymers					729:736	stimuli-responsive functional polymers	699:736	stimuli-responsive functional polymers	699:736	In these systems, by using stimuli-responsive functional polymers in both gels (nanogels and hydrogels), pH, thermal and magnetic-responsive properties can be brought to final soft nanocomposites.
32135252	3	10	theme	entrapped	612:620	arg1	oxide					636:640	entrapped magnetic iron oxide	612:640	entrapped magnetic iron oxide	612:640	The nanogels were introduced during the formation of a hydrogel network to produce nanogel/hydrogel soft nanocomposites, while the biocompatible hydrogel was based on poly (acrylic acid) grafted onto κ-carrageenan polysaccharide with entrapped magnetic iron oxide nanoparticles as crosslinker.
32135252	4	11	theme	magnetic-responsive	793:811	arg1	properties					813:822	magnetic-responsive properties	793:822	magnetic-responsive properties	793:822	In these systems, by using stimuli-responsive functional polymers in both gels (nanogels and hydrogels), pH, thermal and magnetic-responsive properties can be brought to final soft nanocomposites.
32135252	3	12	theme	κ-carrageenan	578:590	arg1	polysaccharide					592:605	κ-carrageenan polysaccharide	578:605	κ-carrageenan polysaccharide with entrapped magnetic iron oxide	578:640	The nanogels were introduced during the formation of a hydrogel network to produce nanogel/hydrogel soft nanocomposites, while the biocompatible hydrogel was based on poly (acrylic acid) grafted onto κ-carrageenan polysaccharide with entrapped magnetic iron oxide nanoparticles as crosslinker.
32135252	9	13	theme	sustained	1387:1395	arg1	model					1405:1409	a sustained release model	1385:1409	a sustained release model that can be extended for other drugs	1385:1446	The results demonstrated a sustained release model that can be extended for other drugs.
32135252	8	14	theme	active	1288:1293	arg1	drug					1295:1298	the active drug	1284:1298	the active drug	1284:1298	pH-dependent release of the active drug can be sustained for >11 days from this soft nanocomposite.
32135252	3	15	theme	biocompatible	509:521	arg1	hydrogel					523:530	the biocompatible hydrogel	505:530	the biocompatible hydrogel	505:530	The nanogels were introduced during the formation of a hydrogel network to produce nanogel/hydrogel soft nanocomposites, while the biocompatible hydrogel was based on poly (acrylic acid) grafted onto κ-carrageenan polysaccharide with entrapped magnetic iron oxide nanoparticles as crosslinker.
32135252	4	16	dep	gels	746:749	arg1	hydrogels					765:773	hydrogels	765:773	hydrogels	765:773	In these systems, by using stimuli-responsive functional polymers in both gels (nanogels and hydrogels), pH, thermal and magnetic-responsive properties can be brought to final soft nanocomposites.
32135252	4	16	dep	gels	746:749	arg1	nanogels					752:759	nanogels	752:759	nanogels	752:759	In these systems, by using stimuli-responsive functional polymers in both gels (nanogels and hydrogels), pH, thermal and magnetic-responsive properties can be brought to final soft nanocomposites.
32135252	1	17	theme	biocompatible	177:189	arg1	system					222:227	a novel biocompatible nanogel/hydrogel nanocomposite system	169:227	a novel biocompatible nanogel/hydrogel nanocomposite system for long-term delivery of bioactive molecules	169:273	This study describes the development of a novel biocompatible nanogel/hydrogel nanocomposite system for long-term delivery of bioactive molecules.
32135252	8	18	from	nanocomposite	1345:1357	arg1	>11 days					1321:1328	>11 days	1321:1328	>11 days from this soft nanocomposite	1321:1357	pH-dependent release of the active drug can be sustained for >11 days from this soft nanocomposite.
32135252	1	19	theme	nanogel/hydrogel	191:206	arg1	system					222:227	a novel biocompatible nanogel/hydrogel nanocomposite system	169:227	a novel biocompatible nanogel/hydrogel nanocomposite system for long-term delivery of bioactive molecules	169:273	This study describes the development of a novel biocompatible nanogel/hydrogel nanocomposite system for long-term delivery of bioactive molecules.
32135252	0	20	theme	responsive	14:23	arg1	nanogel/hydrogel					25:40	Multi-stimuli responsive nanogel/hydrogel	0:40	Multi-stimuli responsive nanogel/hydrogel	0:40	Multi-stimuli responsive nanogel/hydrogel nanocomposites based on κ-carrageenan for prolonged release of levodopa as model drug.
32135252	3	21	dep	network	442:448	arg1	produce					453:459	produce	453:459	to produce nanogel/hydrogel soft nanocomposites	450:496	The nanogels were introduced during the formation of a hydrogel network to produce nanogel/hydrogel soft nanocomposites, while the biocompatible hydrogel was based on poly (acrylic acid) grafted onto κ-carrageenan polysaccharide with entrapped magnetic iron oxide nanoparticles as crosslinker.
32135252	2	22	theme	poly	349:352	arg1	polymerization					331:344	radical polymerization	323:344	radical polymerization of poly(N-isopropylacrylamide)	323:375	To this aim, the nanogels were synthesized via radical polymerization of poly(N-isopropylacrylamide).
32135252	7	23	theme	in	1198:1199	arg1	release					1207:1213	The in vitro release	1194:1213	The in vitro release of L-DOPA	1194:1223	The in vitro release of L-DOPA was investigated at different pHs.
32135252	1	24	theme	nanocomposite	208:220	arg1	system					222:227	a novel biocompatible nanogel/hydrogel nanocomposite system	169:227	a novel biocompatible nanogel/hydrogel nanocomposite system for long-term delivery of bioactive molecules	169:273	This study describes the development of a novel biocompatible nanogel/hydrogel nanocomposite system for long-term delivery of bioactive molecules.
32135252	0	25	theme	Multi-stimuli	0:12	arg1	nanogel/hydrogel					25:40	Multi-stimuli responsive nanogel/hydrogel	0:40	Multi-stimuli responsive nanogel/hydrogel	0:40	Multi-stimuli responsive nanogel/hydrogel nanocomposites based on κ-carrageenan for prolonged release of levodopa as model drug.
32135252	6	26	used	used	1079:1082	arg2	nanogel/hydrogel					1062:1077	This biopolymer based nanogel/hydrogel	1040:1077	This biopolymer based nanogel/hydrogel	1040:1077	This biopolymer based nanogel/hydrogel used as a prolonged releasing drug carrier for levodopa (L-DOPA) as a main drug for treating Parkinson's symptoms.
32135252	3	27	theme	magnetic	622:629	arg1	oxide					636:640	entrapped magnetic iron oxide	612:640	entrapped magnetic iron oxide	612:640	The nanogels were introduced during the formation of a hydrogel network to produce nanogel/hydrogel soft nanocomposites, while the biocompatible hydrogel was based on poly (acrylic acid) grafted onto κ-carrageenan polysaccharide with entrapped magnetic iron oxide nanoparticles as crosslinker.
32135252	1	28	theme	system	222:227	arg1	development					154:164	the development	150:164	the development of a novel biocompatible nanogel/hydrogel nanocomposite system for long-term delivery of bioactive molecules	150:273	This study describes the development of a novel biocompatible nanogel/hydrogel nanocomposite system for long-term delivery of bioactive molecules.
32135252	8	29	theme	soft	1340:1343	arg1	nanocomposite					1345:1357	this soft nanocomposite	1335:1357	this soft nanocomposite	1335:1357	pH-dependent release of the active drug can be sustained for >11 days from this soft nanocomposite.
32135252	5	30	theme	soft	882:885	arg1	nanocomposite					887:899	The obtained soft nanocomposite	869:899	The obtained soft nanocomposite	869:899	The obtained soft nanocomposite was characterized by Fourier transform infrared spectrum (FT-IR), thermogravimetric (TG) analysis, and scanning electron microscopy (SEM).
32135252	5	31	theme	obtained	873:880	arg1	nanocomposite					887:899	The obtained soft nanocomposite	869:899	The obtained soft nanocomposite	869:899	The obtained soft nanocomposite was characterized by Fourier transform infrared spectrum (FT-IR), thermogravimetric (TG) analysis, and scanning electron microscopy (SEM).
32135252	2	32	theme	radical	323:329	arg1	polymerization					331:344	radical polymerization	323:344	radical polymerization of poly(N-isopropylacrylamide)	323:375	To this aim, the nanogels were synthesized via radical polymerization of poly(N-isopropylacrylamide).
32135252	1	33	theme	long-term	233:241	arg1	delivery					243:250	long-term delivery	233:250	long-term delivery of bioactive molecules	233:273	This study describes the development of a novel biocompatible nanogel/hydrogel nanocomposite system for long-term delivery of bioactive molecules.
32135252	3	34	theme	network	442:448	arg1	formation					418:426	the formation	414:426	the formation of a hydrogel network to produce nanogel/hydrogel soft nanocomposites	414:496	The nanogels were introduced during the formation of a hydrogel network to produce nanogel/hydrogel soft nanocomposites, while the biocompatible hydrogel was based on poly (acrylic acid) grafted onto κ-carrageenan polysaccharide with entrapped magnetic iron oxide nanoparticles as crosslinker.
32135252	5	35	dep	transform	930:938	arg1	infrared					940:947	infrared	940:947	transform infrared spectrum (FT-IR), thermogravimetric (TG) analysis, and scanning electron microscopy (SEM)	930:1037	The obtained soft nanocomposite was characterized by Fourier transform infrared spectrum (FT-IR), thermogravimetric (TG) analysis, and scanning electron microscopy (SEM).
32135252	3	36	theme	nanogel/hydrogel	461:476	arg1	nanocomposites					483:496	nanogel/hydrogel soft nanocomposites	461:496	nanogel/hydrogel soft nanocomposites	461:496	The nanogels were introduced during the formation of a hydrogel network to produce nanogel/hydrogel soft nanocomposites, while the biocompatible hydrogel was based on poly (acrylic acid) grafted onto κ-carrageenan polysaccharide with entrapped magnetic iron oxide nanoparticles as crosslinker.
32135252	3	37	theme	soft	478:481	arg1	nanocomposites					483:496	nanogel/hydrogel soft nanocomposites	461:496	nanogel/hydrogel soft nanocomposites	461:496	The nanogels were introduced during the formation of a hydrogel network to produce nanogel/hydrogel soft nanocomposites, while the biocompatible hydrogel was based on poly (acrylic acid) grafted onto κ-carrageenan polysaccharide with entrapped magnetic iron oxide nanoparticles as crosslinker.
32135252	8	38	theme	drug	1295:1298	arg1	release					1273:1279	pH-dependent release	1260:1279	pH-dependent release of the active drug	1260:1298	pH-dependent release of the active drug can be sustained for >11 days from this soft nanocomposite.
32135252	7	39	theme	L-DOPA	1218:1223	arg1	release					1207:1213	The in vitro release	1194:1213	The in vitro release of L-DOPA	1194:1223	The in vitro release of L-DOPA was investigated at different pHs.
32135252	3	40	theme	iron	631:634	arg1	oxide					636:640	entrapped magnetic iron oxide	612:640	entrapped magnetic iron oxide	612:640	The nanogels were introduced during the formation of a hydrogel network to produce nanogel/hydrogel soft nanocomposites, while the biocompatible hydrogel was based on poly (acrylic acid) grafted onto κ-carrageenan polysaccharide with entrapped magnetic iron oxide nanoparticles as crosslinker.
32135252	3	41	theme	hydrogel	433:440	arg1	network					442:448	a hydrogel network	431:448	a hydrogel network to produce nanogel/hydrogel soft nanocomposites	431:496	The nanogels were introduced during the formation of a hydrogel network to produce nanogel/hydrogel soft nanocomposites, while the biocompatible hydrogel was based on poly (acrylic acid) grafted onto κ-carrageenan polysaccharide with entrapped magnetic iron oxide nanoparticles as crosslinker.
32135252	9	42	theme	release	1397:1403	arg1	model					1405:1409	a sustained release model	1385:1409	a sustained release model that can be extended for other drugs	1385:1446	The results demonstrated a sustained release model that can be extended for other drugs.
32135252	5	43	dep	Fourier	922:928	arg1	transform					930:938	transform	930:938	transform infrared spectrum (FT-IR), thermogravimetric (TG) analysis, and scanning electron microscopy (SEM)	930:1037	The obtained soft nanocomposite was characterized by Fourier transform infrared spectrum (FT-IR), thermogravimetric (TG) analysis, and scanning electron microscopy (SEM).
32135252	5	44	theme	thermogravimetric	967:983	arg1	analysis					990:997	thermogravimetric (TG) analysis	967:997	thermogravimetric (TG) analysis	967:997	The obtained soft nanocomposite was characterized by Fourier transform infrared spectrum (FT-IR), thermogravimetric (TG) analysis, and scanning electron microscopy (SEM).
32135252	0	45	dep	nanocomposites	42:55	arg1	based					57:61	based	57:61	nanocomposites based on κ-carrageenan for prolonged release of levodopa as model drug	42:126	Multi-stimuli responsive nanogel/hydrogel nanocomposites based on κ-carrageenan for prolonged release of levodopa as model drug.
32135252	6	46	theme	main	1149:1152	arg1	drug					1154:1157	a main drug	1147:1157	a main drug for treating Parkinson's symptoms	1147:1191	This biopolymer based nanogel/hydrogel used as a prolonged releasing drug carrier for levodopa (L-DOPA) as a main drug for treating Parkinson's symptoms.
32135252	1	47	theme	bioactive	255:263	arg1	molecules					265:273	bioactive molecules	255:273	bioactive molecules	255:273	This study describes the development of a novel biocompatible nanogel/hydrogel nanocomposite system for long-term delivery of bioactive molecules.
32135252	6	48	theme	drug	1109:1112	arg1	carrier					1114:1120	a prolonged releasing drug carrier	1087:1120	a prolonged releasing drug carrier for levodopa (L-DOPA)	1087:1142	This biopolymer based nanogel/hydrogel used as a prolonged releasing drug carrier for levodopa (L-DOPA) as a main drug for treating Parkinson's symptoms.
32135252	1	49	theme	molecules	265:273	arg1	delivery					243:250	long-term delivery	233:250	long-term delivery of bioactive molecules	233:273	This study describes the development of a novel biocompatible nanogel/hydrogel nanocomposite system for long-term delivery of bioactive molecules.
32135252	7	50	theme	different	1245:1253	arg1	pHs					1255:1257	different pHs	1245:1257	different pHs	1245:1257	The in vitro release of L-DOPA was investigated at different pHs.
32135252	4	51	theme	soft	848:851	arg1	nanocomposites					853:866	final soft nanocomposites	842:866	final soft nanocomposites	842:866	In these systems, by using stimuli-responsive functional polymers in both gels (nanogels and hydrogels), pH, thermal and magnetic-responsive properties can be brought to final soft nanocomposites.
32135252	6	52	theme	releasing	1099:1107	arg1	carrier					1114:1120	a prolonged releasing drug carrier	1087:1120	a prolonged releasing drug carrier for levodopa (L-DOPA)	1087:1142	This biopolymer based nanogel/hydrogel used as a prolonged releasing drug carrier for levodopa (L-DOPA) as a main drug for treating Parkinson's symptoms.
32135252	3	53	theme	acrylic	551:557	arg1	poly					545:548	poly	545:548	poly (acrylic acid) grafted onto κ-carrageenan polysaccharide with entrapped magnetic iron oxide nanoparticles as crosslinker	545:669	The nanogels were introduced during the formation of a hydrogel network to produce nanogel/hydrogel soft nanocomposites, while the biocompatible hydrogel was based on poly (acrylic acid) grafted onto κ-carrageenan polysaccharide with entrapped magnetic iron oxide nanoparticles as crosslinker.
32135252	3	53	theme	acrylic	551:557	arg1	acid					559:562	acrylic acid	551:562	acrylic acid	551:562	The nanogels were introduced during the formation of a hydrogel network to produce nanogel/hydrogel soft nanocomposites, while the biocompatible hydrogel was based on poly (acrylic acid) grafted onto κ-carrageenan polysaccharide with entrapped magnetic iron oxide nanoparticles as crosslinker.
32135252	4	54	theme	stimuli-responsive	699:716	arg1	polymers					729:736	stimuli-responsive functional polymers	699:736	stimuli-responsive functional polymers	699:736	In these systems, by using stimuli-responsive functional polymers in both gels (nanogels and hydrogels), pH, thermal and magnetic-responsive properties can be brought to final soft nanocomposites.
32135252	6	55	theme	based	1056:1060	arg1	nanogel/hydrogel					1062:1077	This biopolymer based nanogel/hydrogel	1040:1077	This biopolymer based nanogel/hydrogel	1040:1077	This biopolymer based nanogel/hydrogel used as a prolonged releasing drug carrier for levodopa (L-DOPA) as a main drug for treating Parkinson's symptoms.
32135252	0	56	theme	prolonged	84:92	arg1	release					94:100	prolonged release	84:100	prolonged release of levodopa	84:112	Multi-stimuli responsive nanogel/hydrogel nanocomposites based on κ-carrageenan for prolonged release of levodopa as model drug.
32135252	5	57	theme	electron	1013:1020	arg1	SEM					1034:1036	SEM	1034:1036	SEM	1034:1036	The obtained soft nanocomposite was characterized by Fourier transform infrared spectrum (FT-IR), thermogravimetric (TG) analysis, and scanning electron microscopy (SEM).
32135252	5	57	theme	electron	1013:1020	arg1	microscopy					1022:1031	scanning electron microscopy	1004:1031	scanning electron microscopy (SEM)	1004:1037	The obtained soft nanocomposite was characterized by Fourier transform infrared spectrum (FT-IR), thermogravimetric (TG) analysis, and scanning electron microscopy (SEM).
32135252	4	58	theme	final	842:846	arg1	nanocomposites					853:866	final soft nanocomposites	842:866	final soft nanocomposites	842:866	In these systems, by using stimuli-responsive functional polymers in both gels (nanogels and hydrogels), pH, thermal and magnetic-responsive properties can be brought to final soft nanocomposites.
32135252	3	59	with	polysaccharide	592:605	arg1	oxide					636:640	entrapped magnetic iron oxide	612:640	entrapped magnetic iron oxide	612:640	The nanogels were introduced during the formation of a hydrogel network to produce nanogel/hydrogel soft nanocomposites, while the biocompatible hydrogel was based on poly (acrylic acid) grafted onto κ-carrageenan polysaccharide with entrapped magnetic iron oxide nanoparticles as crosslinker.
32135252	6	60	theme	biopolymer	1045:1054	arg1	nanogel/hydrogel					1062:1077	This biopolymer based nanogel/hydrogel	1040:1077	This biopolymer based nanogel/hydrogel	1040:1077	This biopolymer based nanogel/hydrogel used as a prolonged releasing drug carrier for levodopa (L-DOPA) as a main drug for treating Parkinson's symptoms.
34893280	3	0	theme	ionization	689:698	arg1	mode					700:703	positive ionization mode	680:703	positive ionization mode	680:703	The target components were detected in selected ion monitoring (SIM) mode by mass spectrometry with an electrospray ionization (ESI) source operating in positive ionization mode.
34893280	6	1	theme	comprehensive	1074:1086	arg1	protocol					1060:1067	this protocol	1055:1067	this protocol	1055:1067	The results demonstrate that this protocol is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from TCMs.
34893280	6	1	theme	comprehensive	1074:1086	arg1	technique					1102:1110	a comprehensive and effective technique	1072:1110	a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from TCMs	1072:1187	The results demonstrate that this protocol is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from TCMs.
34893280	1	2	theme	baseline	266:273	arg1	separation					275:284	simultaneous baseline separation	253:284	simultaneous baseline separation	253:284	Here, we report a new application using liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) using aldononitrile acetate derivatives for simultaneous baseline separation and detection of eight neutral saccharides, two uronic acids, one ketose, and eight alditols within 14 min.
34893280	0	3	from	analysis	14:21	arg1	TCMs					55:58	TCMs	55:58	TCMs	55:58	Comprehensive analysis of natural polysaccharides from TCMs: a generic approach based on UPLC-MS/MS.
34893280	1	4	theme	liquid	141:146	arg1	spectrometry					181:192	liquid chromatography-electrospray mass spectrometry	141:192	liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS)	141:207	Here, we report a new application using liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) using aldononitrile acetate derivatives for simultaneous baseline separation and detection of eight neutral saccharides, two uronic acids, one ketose, and eight alditols within 14 min.
34893280	1	4	theme	liquid	141:146	arg1	UHPLC-ESI-MS					195:206	UHPLC-ESI-MS	195:206	UHPLC-ESI-MS	195:206	Here, we report a new application using liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) using aldononitrile acetate derivatives for simultaneous baseline separation and detection of eight neutral saccharides, two uronic acids, one ketose, and eight alditols within 14 min.
34893280	3	5	located	detected	554:561	arg2	components					538:547	The target components	527:547	The target components	527:547	The target components were detected in selected ion monitoring (SIM) mode by mass spectrometry with an electrospray ionization (ESI) source operating in positive ionization mode.
34893280	3	5	located	detected	554:561	arg1	mode					596:599	selected ion monitoring (SIM) mode	566:599	selected ion monitoring (SIM) mode	566:599	The target components were detected in selected ion monitoring (SIM) mode by mass spectrometry with an electrospray ionization (ESI) source operating in positive ionization mode.
34893280	2	6	theme	mobile	491:496	arg1	phase					498:502	the mobile phase	487:502	the mobile phase with gradient elution	487:524	The separation was performed on a Cortecs C₁₈ column using acetonitrile (A) and water (B) as the mobile phase with gradient elution.
34893280	2	6	theme	mobile	491:496	arg1	water					474:478	water	474:478	water (B)	474:482	The separation was performed on a Cortecs C₁₈ column using acetonitrile (A) and water (B) as the mobile phase with gradient elution.
34893280	2	6	theme	mobile	491:496	arg1	acetonitrile					453:464	acetonitrile	453:464	acetonitrile (A)	453:468	The separation was performed on a Cortecs C₁₈ column using acetonitrile (A) and water (B) as the mobile phase with gradient elution.
34893280	5	7	theme	cyrtonema	959:967	arg1	determination					930:942	determination	930:942	quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides	832:1004	The UHPLC-ESI-MS method was used for quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides in polysaccharides.
34893280	5	7	theme	cyrtonema	959:967	arg1	analysis					845:852	quantitative analysis	832:852	quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides	832:1004	The UHPLC-ESI-MS method was used for quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides in polysaccharides.
34893280	6	8	theme	quantitative	1132:1143	arg1	analysis					1145:1152	qualitative and quantitative analysis	1116:1152	qualitative and quantitative analysis of plant polysaccharides from TCMs	1116:1187	The results demonstrate that this protocol is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from TCMs.
34893280	6	9	theme	plant	1157:1161	arg1	polysaccharides					1163:1177	plant polysaccharides	1157:1177	plant polysaccharides from TCMs	1157:1187	The results demonstrate that this protocol is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from TCMs.
34893280	3	10	theme	ionization	643:652	arg1	source					660:665	an electrospray ionization (ESI) source	627:665	an electrospray ionization (ESI) source operating in positive ionization mode	627:703	The target components were detected in selected ion monitoring (SIM) mode by mass spectrometry with an electrospray ionization (ESI) source operating in positive ionization mode.
34893280	3	11	with	spectrometry	609:620	arg1	source					660:665	an electrospray ionization (ESI) source	627:665	an electrospray ionization (ESI) source operating in positive ionization mode	627:703	The target components were detected in selected ion monitoring (SIM) mode by mass spectrometry with an electrospray ionization (ESI) source operating in positive ionization mode.
34893280	6	12	from	TCMs	1184:1187	arg1	analysis					1145:1152	qualitative and quantitative analysis	1116:1152	qualitative and quantitative analysis of plant polysaccharides from TCMs	1116:1187	The results demonstrate that this protocol is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from TCMs.
34893280	6	12	from	TCMs	1184:1187	arg1	polysaccharides					1163:1177	plant polysaccharides	1157:1177	plant polysaccharides from TCMs	1157:1187	The results demonstrate that this protocol is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from TCMs.
34893280	0	13	from	TCMs	55:58	arg1	analysis					14:21	Comprehensive analysis	0:21	Comprehensive analysis of natural polysaccharides from TCMs: a generic approach based on UPLC-MS/MS.	0:99	Comprehensive analysis of natural polysaccharides from TCMs: a generic approach based on UPLC-MS/MS.
34893280	0	13	from	TCMs	55:58	arg1	polysaccharides					34:48	natural polysaccharides	26:48	natural polysaccharides from TCMs	26:58	Comprehensive analysis of natural polysaccharides from TCMs: a generic approach based on UPLC-MS/MS.
34893280	5	14	from	analysis	845:852	arg1	extracts					885:892	water extracts	879:892	water extracts of Crataegus pinnatifida	879:917	The UHPLC-ESI-MS method was used for quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides in polysaccharides.
34893280	1	15	theme	chromatography-electrospray	148:174	arg1	spectrometry					181:192	liquid chromatography-electrospray mass spectrometry	141:192	liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS)	141:207	Here, we report a new application using liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) using aldononitrile acetate derivatives for simultaneous baseline separation and detection of eight neutral saccharides, two uronic acids, one ketose, and eight alditols within 14 min.
34893280	1	15	theme	chromatography-electrospray	148:174	arg1	UHPLC-ESI-MS					195:206	UHPLC-ESI-MS	195:206	UHPLC-ESI-MS	195:206	Here, we report a new application using liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) using aldononitrile acetate derivatives for simultaneous baseline separation and detection of eight neutral saccharides, two uronic acids, one ketose, and eight alditols within 14 min.
34893280	1	16	theme	alditols	370:377	arg1	separation					275:284	simultaneous baseline separation	253:284	simultaneous baseline separation	253:284	Here, we report a new application using liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) using aldononitrile acetate derivatives for simultaneous baseline separation and detection of eight neutral saccharides, two uronic acids, one ketose, and eight alditols within 14 min.
34893280	1	16	theme	alditols	370:377	arg1	detection					290:298	detection	290:298	detection	290:298	Here, we report a new application using liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) using aldononitrile acetate derivatives for simultaneous baseline separation and detection of eight neutral saccharides, two uronic acids, one ketose, and eight alditols within 14 min.
34893280	4	17	theme	results	786:792	arg1	validity					770:777	the validity	766:777	the validity of the results	766:792	A comparison with traditional methods was used to determine the validity of the results.
34893280	4	18	theme	traditional	724:734	arg1	methods					736:742	traditional methods	724:742	traditional methods	724:742	A comparison with traditional methods was used to determine the validity of the results.
34893280	1	19	theme	mass	176:179	arg1	spectrometry					181:192	liquid chromatography-electrospray mass spectrometry	141:192	liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS)	141:207	Here, we report a new application using liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) using aldononitrile acetate derivatives for simultaneous baseline separation and detection of eight neutral saccharides, two uronic acids, one ketose, and eight alditols within 14 min.
34893280	1	19	theme	mass	176:179	arg1	UHPLC-ESI-MS					195:206	UHPLC-ESI-MS	195:206	UHPLC-ESI-MS	195:206	Here, we report a new application using liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) using aldononitrile acetate derivatives for simultaneous baseline separation and detection of eight neutral saccharides, two uronic acids, one ketose, and eight alditols within 14 min.
34893280	4	20	with	comparison	708:717	arg1	methods					736:742	traditional methods	724:742	traditional methods	724:742	A comparison with traditional methods was used to determine the validity of the results.
34893280	5	21	from	extracts	885:892	arg1	determination					930:942	determination	930:942	quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides	832:1004	The UHPLC-ESI-MS method was used for quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides in polysaccharides.
34893280	5	21	from	extracts	885:892	arg1	analysis					845:852	quantitative analysis	832:852	quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides	832:1004	The UHPLC-ESI-MS method was used for quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides in polysaccharides.
34893280	3	22	theme	target	531:536	arg1	components					538:547	The target components	527:547	The target components	527:547	The target components were detected in selected ion monitoring (SIM) mode by mass spectrometry with an electrospray ionization (ESI) source operating in positive ionization mode.
34893280	3	23	theme	positive	680:687	arg1	mode					700:703	positive ionization mode	680:703	positive ionization mode	680:703	The target components were detected in selected ion monitoring (SIM) mode by mass spectrometry with an electrospray ionization (ESI) source operating in positive ionization mode.
34893280	0	24	theme	Comprehensive	0:12	arg1	analysis					14:21	Comprehensive analysis	0:21	Comprehensive analysis of natural polysaccharides from TCMs: a generic approach based on UPLC-MS/MS.	0:99	Comprehensive analysis of natural polysaccharides from TCMs: a generic approach based on UPLC-MS/MS.
34893280	5	25	from	carbohydrates	862:874	arg1	extracts					885:892	water extracts	879:892	water extracts of Crataegus pinnatifida	879:917	The UHPLC-ESI-MS method was used for quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides in polysaccharides.
34893280	5	26	used	used	823:826	arg2	method					812:817	The UHPLC-ESI-MS method	795:817	The UHPLC-ESI-MS method	795:817	The UHPLC-ESI-MS method was used for quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides in polysaccharides.
34893280	6	27	from	analysis	1145:1152	arg1	TCMs					1184:1187	TCMs	1184:1187	TCMs	1184:1187	The results demonstrate that this protocol is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from TCMs.
34893280	0	28	theme	natural	26:32	arg1	polysaccharides					34:48	natural polysaccharides	26:48	natural polysaccharides from TCMs	26:58	Comprehensive analysis of natural polysaccharides from TCMs: a generic approach based on UPLC-MS/MS.
34893280	3	29	theme	ion	575:577	arg1	mode					596:599	selected ion monitoring (SIM) mode	566:599	selected ion monitoring (SIM) mode	566:599	The target components were detected in selected ion monitoring (SIM) mode by mass spectrometry with an electrospray ionization (ESI) source operating in positive ionization mode.
34893280	5	30	theme	pinnatifida	907:917	arg1	extracts					885:892	water extracts	879:892	water extracts of Crataegus pinnatifida	879:917	The UHPLC-ESI-MS method was used for quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides in polysaccharides.
34893280	5	31	theme	ganoderma	980:988	arg1	monosaccharides					990:1004	ganoderma monosaccharides	980:1004	ganoderma monosaccharides	980:1004	The UHPLC-ESI-MS method was used for quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides in polysaccharides.
34893280	2	32	theme	Cortecs	428:434	arg1	column					440:445	a Cortecs C₁₈ column	426:445	a Cortecs C₁₈ column using acetonitrile (A) and water (B) as the mobile phase with gradient elution	426:524	The separation was performed on a Cortecs C₁₈ column using acetonitrile (A) and water (B) as the mobile phase with gradient elution.
34893280	1	33	theme	neutral	309:315	arg1	saccharides					317:327	eight neutral saccharides	303:327	eight neutral saccharides	303:327	Here, we report a new application using liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) using aldononitrile acetate derivatives for simultaneous baseline separation and detection of eight neutral saccharides, two uronic acids, one ketose, and eight alditols within 14 min.
34893280	2	34	theme	gradient	509:516	arg1	elution					518:524	gradient elution	509:524	gradient elution	509:524	The separation was performed on a Cortecs C₁₈ column using acetonitrile (A) and water (B) as the mobile phase with gradient elution.
34893280	5	35	theme	free	857:860	arg1	carbohydrates					862:874	free carbohydrates	857:874	free carbohydrates in water extracts of Crataegus pinnatifida	857:917	The UHPLC-ESI-MS method was used for quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides in polysaccharides.
34893280	5	36	theme	quantitative	832:843	arg1	analysis					845:852	quantitative analysis	832:852	quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides	832:1004	The UHPLC-ESI-MS method was used for quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides in polysaccharides.
34893280	1	37	theme	saccharides	317:327	arg1	separation					275:284	simultaneous baseline separation	253:284	simultaneous baseline separation	253:284	Here, we report a new application using liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) using aldononitrile acetate derivatives for simultaneous baseline separation and detection of eight neutral saccharides, two uronic acids, one ketose, and eight alditols within 14 min.
34893280	1	37	theme	saccharides	317:327	arg1	detection					290:298	detection	290:298	detection	290:298	Here, we report a new application using liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) using aldononitrile acetate derivatives for simultaneous baseline separation and detection of eight neutral saccharides, two uronic acids, one ketose, and eight alditols within 14 min.
34893280	3	38	theme	mass	604:607	arg1	spectrometry					609:620	mass spectrometry	604:620	mass spectrometry with an electrospray ionization (ESI) source operating in positive ionization mode	604:703	The target components were detected in selected ion monitoring (SIM) mode by mass spectrometry with an electrospray ionization (ESI) source operating in positive ionization mode.
34893280	1	39	theme	ketose	352:357	arg1	separation					275:284	simultaneous baseline separation	253:284	simultaneous baseline separation	253:284	Here, we report a new application using liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) using aldononitrile acetate derivatives for simultaneous baseline separation and detection of eight neutral saccharides, two uronic acids, one ketose, and eight alditols within 14 min.
34893280	1	39	theme	ketose	352:357	arg1	detection					290:298	detection	290:298	detection	290:298	Here, we report a new application using liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) using aldononitrile acetate derivatives for simultaneous baseline separation and detection of eight neutral saccharides, two uronic acids, one ketose, and eight alditols within 14 min.
34893280	4	40	used	used	748:751	arg2	comparison					708:717	A comparison	706:717	A comparison with traditional methods	706:742	A comparison with traditional methods was used to determine the validity of the results.
34893280	0	41	theme	polysaccharides	34:48	arg1	analysis					14:21	Comprehensive analysis	0:21	Comprehensive analysis of natural polysaccharides from TCMs: a generic approach based on UPLC-MS/MS.	0:99	Comprehensive analysis of natural polysaccharides from TCMs: a generic approach based on UPLC-MS/MS.
34893280	5	42	dep	cyrtonema	959:967	arg1	monosaccharides					990:1004	ganoderma monosaccharides	980:1004	ganoderma monosaccharides	980:1004	The UHPLC-ESI-MS method was used for quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides in polysaccharides.
34893280	2	43	theme	C₁₈	436:438	arg1	column					440:445	a Cortecs C₁₈ column	426:445	a Cortecs C₁₈ column using acetonitrile (A) and water (B) as the mobile phase with gradient elution	426:524	The separation was performed on a Cortecs C₁₈ column using acetonitrile (A) and water (B) as the mobile phase with gradient elution.
34893280	1	44	theme	aldononitrile	215:227	arg1	derivatives					237:247	aldononitrile acetate derivatives	215:247	aldononitrile acetate derivatives for simultaneous baseline separation and detection of eight neutral saccharides, two uronic acids, one ketose, and eight alditols within 14 min	215:391	Here, we report a new application using liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) using aldononitrile acetate derivatives for simultaneous baseline separation and detection of eight neutral saccharides, two uronic acids, one ketose, and eight alditols within 14 min.
34893280	5	45	theme	Glossy	973:978	arg1	determination					930:942	determination	930:942	quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides	832:1004	The UHPLC-ESI-MS method was used for quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides in polysaccharides.
34893280	5	45	theme	Glossy	973:978	arg1	analysis					845:852	quantitative analysis	832:852	quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides	832:1004	The UHPLC-ESI-MS method was used for quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides in polysaccharides.
34893280	6	46	theme	effective	1092:1100	arg1	protocol					1060:1067	this protocol	1055:1067	this protocol	1055:1067	The results demonstrate that this protocol is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from TCMs.
34893280	6	46	theme	effective	1092:1100	arg1	technique					1102:1110	a comprehensive and effective technique	1072:1110	a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from TCMs	1072:1187	The results demonstrate that this protocol is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from TCMs.
34893280	5	47	theme	carbohydrates	862:874	arg1	determination					930:942	determination	930:942	quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides	832:1004	The UHPLC-ESI-MS method was used for quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides in polysaccharides.
34893280	5	47	theme	carbohydrates	862:874	arg1	analysis					845:852	quantitative analysis	832:852	quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides	832:1004	The UHPLC-ESI-MS method was used for quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides in polysaccharides.
34893280	1	48	theme	uronic	334:339	arg1	acids					341:345	two uronic acids	330:345	two uronic acids	330:345	Here, we report a new application using liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) using aldononitrile acetate derivatives for simultaneous baseline separation and detection of eight neutral saccharides, two uronic acids, one ketose, and eight alditols within 14 min.
34893280	6	49	theme	qualitative	1116:1126	arg1	analysis					1145:1152	qualitative and quantitative analysis	1116:1152	qualitative and quantitative analysis of plant polysaccharides from TCMs	1116:1187	The results demonstrate that this protocol is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from TCMs.
34893280	1	50	theme	acids	341:345	arg1	separation					275:284	simultaneous baseline separation	253:284	simultaneous baseline separation	253:284	Here, we report a new application using liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) using aldononitrile acetate derivatives for simultaneous baseline separation and detection of eight neutral saccharides, two uronic acids, one ketose, and eight alditols within 14 min.
34893280	1	50	theme	acids	341:345	arg1	detection					290:298	detection	290:298	detection	290:298	Here, we report a new application using liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) using aldononitrile acetate derivatives for simultaneous baseline separation and detection of eight neutral saccharides, two uronic acids, one ketose, and eight alditols within 14 min.
34893280	3	51	theme	electrospray	630:641	arg1	source					660:665	an electrospray ionization (ESI) source	627:665	an electrospray ionization (ESI) source operating in positive ionization mode	627:703	The target components were detected in selected ion monitoring (SIM) mode by mass spectrometry with an electrospray ionization (ESI) source operating in positive ionization mode.
34893280	3	52	theme	selected	566:573	arg1	mode					596:599	selected ion monitoring (SIM) mode	566:599	selected ion monitoring (SIM) mode	566:599	The target components were detected in selected ion monitoring (SIM) mode by mass spectrometry with an electrospray ionization (ESI) source operating in positive ionization mode.
34893280	5	53	theme	water	879:883	arg1	extracts					885:892	water extracts	879:892	water extracts of Crataegus pinnatifida	879:917	The UHPLC-ESI-MS method was used for quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides in polysaccharides.
34893280	2	54	with	phase	498:502	arg1	elution					518:524	gradient elution	509:524	gradient elution	509:524	The separation was performed on a Cortecs C₁₈ column using acetonitrile (A) and water (B) as the mobile phase with gradient elution.
34893280	1	55	theme	acetate	229:235	arg1	derivatives					237:247	aldononitrile acetate derivatives	215:247	aldononitrile acetate derivatives for simultaneous baseline separation and detection of eight neutral saccharides, two uronic acids, one ketose, and eight alditols within 14 min	215:391	Here, we report a new application using liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) using aldononitrile acetate derivatives for simultaneous baseline separation and detection of eight neutral saccharides, two uronic acids, one ketose, and eight alditols within 14 min.
34893280	6	56	theme	polysaccharides	1163:1177	arg1	analysis					1145:1152	qualitative and quantitative analysis	1116:1152	qualitative and quantitative analysis of plant polysaccharides from TCMs	1116:1187	The results demonstrate that this protocol is a comprehensive and effective technique for qualitative and quantitative analysis of plant polysaccharides from TCMs.
34893280	3	57	theme	SIM	591:593	arg1	mode					596:599	selected ion monitoring (SIM) mode	566:599	selected ion monitoring (SIM) mode	566:599	The target components were detected in selected ion monitoring (SIM) mode by mass spectrometry with an electrospray ionization (ESI) source operating in positive ionization mode.
34893280	0	58	theme	generic	63:69	arg1	approach					71:78	a generic approach	61:78	Comprehensive analysis of natural polysaccharides from TCMs: a generic approach based on UPLC-MS/MS.	0:99	Comprehensive analysis of natural polysaccharides from TCMs: a generic approach based on UPLC-MS/MS.
34893280	5	59	theme	UHPLC-ESI-MS	799:810	arg1	method					812:817	The UHPLC-ESI-MS method	795:817	The UHPLC-ESI-MS method	795:817	The UHPLC-ESI-MS method was used for quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides in polysaccharides.
34893280	0	60	dep	analysis	14:21	arg1	approach					71:78	a generic approach	61:78	Comprehensive analysis of natural polysaccharides from TCMs: a generic approach based on UPLC-MS/MS.	0:99	Comprehensive analysis of natural polysaccharides from TCMs: a generic approach based on UPLC-MS/MS.
34893280	1	61	theme	new	119:121	arg1	application					123:133	a new application	117:133	a new application using liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS)	117:207	Here, we report a new application using liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) using aldononitrile acetate derivatives for simultaneous baseline separation and detection of eight neutral saccharides, two uronic acids, one ketose, and eight alditols within 14 min.
34893280	3	62	theme	ESI	655:657	arg1	source					660:665	an electrospray ionization (ESI) source	627:665	an electrospray ionization (ESI) source operating in positive ionization mode	627:703	The target components were detected in selected ion monitoring (SIM) mode by mass spectrometry with an electrospray ionization (ESI) source operating in positive ionization mode.
34893280	3	63	theme	monitoring	579:588	arg1	mode					596:599	selected ion monitoring (SIM) mode	566:599	selected ion monitoring (SIM) mode	566:599	The target components were detected in selected ion monitoring (SIM) mode by mass spectrometry with an electrospray ionization (ESI) source operating in positive ionization mode.
34893280	5	64	from	determination	930:942	arg1	extracts					885:892	water extracts	879:892	water extracts of Crataegus pinnatifida	879:917	The UHPLC-ESI-MS method was used for quantitative analysis of free carbohydrates in water extracts of Crataegus pinnatifida as well as determination of Polygonatum cyrtonema and Glossy ganoderma monosaccharides in polysaccharides.
34893280	1	65	theme	simultaneous	253:264	arg1	separation					275:284	simultaneous baseline separation	253:284	simultaneous baseline separation	253:284	Here, we report a new application using liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) using aldononitrile acetate derivatives for simultaneous baseline separation and detection of eight neutral saccharides, two uronic acids, one ketose, and eight alditols within 14 min.
33722892	1	0	from	common	339:344	arg1	Africa					358:363	Africa	358:363	Africa	358:363	Xpert MTB/RIF rapidly detects resistance to rifampicin (RR); however, this test misses I491F-RR conferring rpoB mutation, common in southern Africa.
33722892	5	1	from	indirect-DST	849:860	arg1	media					897:901	Löwenstein-Jensen or 7H11 solid media	865:901	media	897:901	TLA results were compared at the Reference Laboratory in Antwerp with indirect-DST on Löwenstein-Jensen or 7H11 solid media and additional phenotypic and genotypic testing to resolve discordance.
33722892	5	1	from	indirect-DST	849:860	arg1	testing					943:949	additional phenotypic and genotypic testing	907:949	additional phenotypic and genotypic testing	907:949	TLA results were compared at the Reference Laboratory in Antwerp with indirect-DST on Löwenstein-Jensen or 7H11 solid media and additional phenotypic and genotypic testing to resolve discordance.
33722892	3	2	theme	thin-layer	511:520	arg1	DST					569:571	DST	569:571	DST	569:571	We aimed to investigate the ability of thin-layer agar (TLA) direct drug-susceptibility testing (DST) to detect MTB and its drug-resistance profiles in field conditions in Eswatini.
33722892	3	2	theme	thin-layer	511:520	arg1	testing					560:566	thin-layer agar (TLA) direct drug-susceptibility testing	511:566	thin-layer agar (TLA) direct drug-susceptibility testing (DST)	511:572	We aimed to investigate the ability of thin-layer agar (TLA) direct drug-susceptibility testing (DST) to detect MTB and its drug-resistance profiles in field conditions in Eswatini.
33722892	9	3	theme	I491F	1495:1499	arg1	mutation					1501:1508	the I491F mutation	1491:1508	the I491F mutation	1491:1508	Eight isolates, 28.6% of all RR-confirmed isolates, carried the I491F mutation, all detected by TLA.
33722892	9	3	theme	I491F	1495:1499	arg1	all					1511:1513	all	1511:1513	all	1511:1513	Eight isolates, 28.6% of all RR-confirmed isolates, carried the I491F mutation, all detected by TLA.
33722892	8	4	theme	Xpert	1416:1420	arg1	MTB/RIF					1422:1428	Xpert MTB/RIF	1416:1428	Xpert MTB/RIF	1416:1428	Within a median of 18.4 days, TLA detected RR with 93.0% sensitivity (95% confidence interval [CI], 77.4 to 98.0) and 99.4% specificity (95% CI, 96.7 to 99.9) versus 62.5% (95% CI, 42.7 to 78.8) and 99.3% (95% CI, 96.2 to 99.9) for Xpert MTB/RIF.
33722892	6	5	theme	%	1041:1041	arg1	detection					1012:1020	MTB detection	1008:1020	MTB detection of 7.1% versus 10.0% for Xpert MTB/RIF	1008:1059	TLA showed a positivity rate for MTB detection of 7.1% versus 10.0% for Xpert MTB/RIF.
33722892	8	6	dep	RR	1227:1228	arg1	specificity					1308:1318	specificity	1308:1318	specificity	1308:1318	Within a median of 18.4 days, TLA detected RR with 93.0% sensitivity (95% confidence interval [CI], 77.4 to 98.0) and 99.4% specificity (95% CI, 96.7 to 99.9) versus 62.5% (95% CI, 42.7 to 78.8) and 99.3% (95% CI, 96.2 to 99.9) for Xpert MTB/RIF.
33722892	5	7	theme	solid	891:895	arg1	media					897:901	Löwenstein-Jensen or 7H11 solid media	865:901	media	897:901	TLA results were compared at the Reference Laboratory in Antwerp with indirect-DST on Löwenstein-Jensen or 7H11 solid media and additional phenotypic and genotypic testing to resolve discordance.
33722892	3	8	theme	agar	522:525	arg1	DST					569:571	DST	569:571	DST	569:571	We aimed to investigate the ability of thin-layer agar (TLA) direct drug-susceptibility testing (DST) to detect MTB and its drug-resistance profiles in field conditions in Eswatini.
33722892	3	8	theme	agar	522:525	arg1	testing					560:566	thin-layer agar (TLA) direct drug-susceptibility testing	511:566	thin-layer agar (TLA) direct drug-susceptibility testing (DST)	511:572	We aimed to investigate the ability of thin-layer agar (TLA) direct drug-susceptibility testing (DST) to detect MTB and its drug-resistance profiles in field conditions in Eswatini.
33722892	8	9	dep	99.9	1337:1340	arg1	to					1334:1335	to	1334:1335	to	1334:1335	Within a median of 18.4 days, TLA detected RR with 93.0% sensitivity (95% confidence interval [CI], 77.4 to 98.0) and 99.4% specificity (95% CI, 96.7 to 99.9) versus 62.5% (95% CI, 42.7 to 78.8) and 99.3% (95% CI, 96.2 to 99.9) for Xpert MTB/RIF.
33722892	0	10	theme	tuberculosis	120:131	arg1	Growth					133:138	Mycobacterium tuberculosis Growth	106:138	Mycobacterium tuberculosis Growth	106:138	Thin-Layer-Agar-Based Direct Phenotypic Drug Susceptibility Testing on Sputum in Eswatini Rapidly Detects Mycobacterium tuberculosis Growth and Rifampicin Resistance Otherwise Missed by WHO-Endorsed Diagnostic Tests.
33722892	7	11	theme	4,547	1076:1080	arg1	samples					1082:1088	4,547 samples	1076:1088	4,547 samples included in the study	1076:1110	Of a total of 4,547 samples included in the study, 200 isolates were available for comparison to the composite reference.
33722892	3	12	theme	drug-susceptibility	540:558	arg1	DST					569:571	DST	569:571	DST	569:571	We aimed to investigate the ability of thin-layer agar (TLA) direct drug-susceptibility testing (DST) to detect MTB and its drug-resistance profiles in field conditions in Eswatini.
33722892	3	12	theme	drug-susceptibility	540:558	arg1	testing					560:566	thin-layer agar (TLA) direct drug-susceptibility testing	511:566	thin-layer agar (TLA) direct drug-susceptibility testing (DST)	511:572	We aimed to investigate the ability of thin-layer agar (TLA) direct drug-susceptibility testing (DST) to detect MTB and its drug-resistance profiles in field conditions in Eswatini.
33722892	6	13	theme	positivity	988:997	arg1	rate					999:1002	a positivity rate	986:1002	a positivity rate for MTB detection of 7.1% versus 10.0% for Xpert MTB/RIF	986:1059	TLA showed a positivity rate for MTB detection of 7.1% versus 10.0% for Xpert MTB/RIF.
33722892	0	14	theme	Mycobacterium	106:118	arg1	Growth					133:138	Mycobacterium tuberculosis Growth	106:138	Mycobacterium tuberculosis Growth	106:138	Thin-Layer-Agar-Based Direct Phenotypic Drug Susceptibility Testing on Sputum in Eswatini Rapidly Detects Mycobacterium tuberculosis Growth and Rifampicin Resistance Otherwise Missed by WHO-Endorsed Diagnostic Tests.
33722892	7	15	theme	samples	1082:1088	arg1	total					1067:1071	a total	1065:1071	a total of 4,547 samples included in the study	1065:1110	Of a total of 4,547 samples included in the study, 200 isolates were available for comparison to the composite reference.
33722892	11	16	theme	field	1654:1658	arg1	conditions					1660:1669	field conditions	1654:1669	field conditions	1654:1669	In field conditions, TLA rapidly detects RR, and in this specific setting, it contributed to detection of additional RR patients over Xpert MTB/RIF, mainly but not exclusively due to I491F.
33722892	3	17	theme	testing	560:566	arg1	ability					500:506	the ability	496:506	the ability of thin-layer agar (TLA) direct drug-susceptibility testing (DST) to detect MTB and its drug-resistance profiles in field conditions in Eswatini	496:651	We aimed to investigate the ability of thin-layer agar (TLA) direct drug-susceptibility testing (DST) to detect MTB and its drug-resistance profiles in field conditions in Eswatini.
33722892	10	18	dep	%	1630:1630	arg1	99.9					1644:1647	99.9	1644:1647	99.9	1644:1647	TLA also correctly identified 183 of the 184 ofloxacin-susceptible isolates (99.5% specificity; 95% CI, 97.0 to 99.9).
33722892	8	19	dep	99.9	1406:1409	arg1	to					1403:1404	to	1403:1404	to	1403:1404	Within a median of 18.4 days, TLA detected RR with 93.0% sensitivity (95% confidence interval [CI], 77.4 to 98.0) and 99.4% specificity (95% CI, 96.7 to 99.9) versus 62.5% (95% CI, 42.7 to 78.8) and 99.3% (95% CI, 96.2 to 99.9) for Xpert MTB/RIF.
33722892	7	20	theme	total	1067:1071	arg1	available					1131:1139	available	1131:1139	available	1131:1139	Of a total of 4,547 samples included in the study, 200 isolates were available for comparison to the composite reference.
33722892	8	21	theme	93.0	1235:1238	arg1	%					1239:1239	%	1239:1239	%	1239:1239	Within a median of 18.4 days, TLA detected RR with 93.0% sensitivity (95% confidence interval [CI], 77.4 to 98.0) and 99.4% specificity (95% CI, 96.7 to 99.9) versus 62.5% (95% CI, 42.7 to 78.8) and 99.3% (95% CI, 96.2 to 99.9) for Xpert MTB/RIF.
33722892	11	22	theme	specific	1708:1715	arg1	setting					1717:1723	this specific setting	1703:1723	this specific setting	1703:1723	In field conditions, TLA rapidly detects RR, and in this specific setting, it contributed to detection of additional RR patients over Xpert MTB/RIF, mainly but not exclusively due to I491F.
33722892	8	23	dep	98.0	1292:1295	arg1	to					1289:1290	to	1289:1290	to	1289:1290	Within a median of 18.4 days, TLA detected RR with 93.0% sensitivity (95% confidence interval [CI], 77.4 to 98.0) and 99.4% specificity (95% CI, 96.7 to 99.9) versus 62.5% (95% CI, 42.7 to 78.8) and 99.3% (95% CI, 96.2 to 99.9) for Xpert MTB/RIF.
33722892	8	24	theme	interval	1269:1276	arg1	[CI					1278:1280	95% confidence interval [CI	1254:1280	95% confidence interval [CI	1254:1280	Within a median of 18.4 days, TLA detected RR with 93.0% sensitivity (95% confidence interval [CI], 77.4 to 98.0) and 99.4% specificity (95% CI, 96.7 to 99.9) versus 62.5% (95% CI, 42.7 to 78.8) and 99.3% (95% CI, 96.2 to 99.9) for Xpert MTB/RIF.
33722892	8	24	theme	interval	1269:1276	arg1	RR					1227:1228	RR	1227:1228	RR	1227:1228	Within a median of 18.4 days, TLA detected RR with 93.0% sensitivity (95% confidence interval [CI], 77.4 to 98.0) and 99.4% specificity (95% CI, 96.7 to 99.9) versus 62.5% (95% CI, 42.7 to 78.8) and 99.3% (95% CI, 96.2 to 99.9) for Xpert MTB/RIF.
33722892	0	25	from	Testing	60:66	arg1	Eswatini					81:88	Eswatini	81:88	Eswatini	81:88	Thin-Layer-Agar-Based Direct Phenotypic Drug Susceptibility Testing on Sputum in Eswatini Rapidly Detects Mycobacterium tuberculosis Growth and Rifampicin Resistance Otherwise Missed by WHO-Endorsed Diagnostic Tests.
33722892	0	25	from	Testing	60:66	arg1	Sputum					71:76	Sputum	71:76	Sputum	71:76	Thin-Layer-Agar-Based Direct Phenotypic Drug Susceptibility Testing on Sputum in Eswatini Rapidly Detects Mycobacterium tuberculosis Growth and Rifampicin Resistance Otherwise Missed by WHO-Endorsed Diagnostic Tests.
33722892	3	26	theme	direct	533:538	arg1	DST					569:571	DST	569:571	DST	569:571	We aimed to investigate the ability of thin-layer agar (TLA) direct drug-susceptibility testing (DST) to detect MTB and its drug-resistance profiles in field conditions in Eswatini.
33722892	3	26	theme	direct	533:538	arg1	testing					560:566	thin-layer agar (TLA) direct drug-susceptibility testing	511:566	thin-layer agar (TLA) direct drug-susceptibility testing (DST)	511:572	We aimed to investigate the ability of thin-layer agar (TLA) direct drug-susceptibility testing (DST) to detect MTB and its drug-resistance profiles in field conditions in Eswatini.
33722892	11	27	theme	additional	1757:1766	arg1	patients					1771:1778	additional RR patients	1757:1778	additional RR patients over Xpert MTB/RIF	1757:1797	In field conditions, TLA rapidly detects RR, and in this specific setting, it contributed to detection of additional RR patients over Xpert MTB/RIF, mainly but not exclusively due to I491F.
33722892	1	28	theme	Xpert	217:221	arg1	MTB/RIF					223:229	Xpert MTB/RIF	217:229	Xpert MTB/RIF	217:229	Xpert MTB/RIF rapidly detects resistance to rifampicin (RR); however, this test misses I491F-RR conferring rpoB mutation, common in southern Africa.
33722892	0	29	theme	Direct	22:27	arg1	Testing					60:66	Thin-Layer-Agar-Based Direct Phenotypic Drug Susceptibility Testing	0:66	Thin-Layer-Agar-Based Direct Phenotypic Drug Susceptibility Testing on Sputum in Eswatini	0:88	Thin-Layer-Agar-Based Direct Phenotypic Drug Susceptibility Testing on Sputum in Eswatini Rapidly Detects Mycobacterium tuberculosis Growth and Rifampicin Resistance Otherwise Missed by WHO-Endorsed Diagnostic Tests.
33722892	5	30	theme	genotypic	933:941	arg1	testing					943:949	additional phenotypic and genotypic testing	907:949	additional phenotypic and genotypic testing	907:949	TLA results were compared at the Reference Laboratory in Antwerp with indirect-DST on Löwenstein-Jensen or 7H11 solid media and additional phenotypic and genotypic testing to resolve discordance.
33722892	10	31	dep	identified	1551:1560	arg1	%					1630:1630	99.5% specificity; 95% CI	1609:1633	99.5% specificity; 95% CI	1609:1633	TLA also correctly identified 183 of the 184 ofloxacin-susceptible isolates (99.5% specificity; 95% CI, 97.0 to 99.9).
33722892	6	32	theme	Xpert	1047:1051	arg1	MTB/RIF					1053:1059	Xpert MTB/RIF	1047:1059	Xpert MTB/RIF	1047:1059	TLA showed a positivity rate for MTB detection of 7.1% versus 10.0% for Xpert MTB/RIF.
33722892	0	33	theme	Thin-Layer-Agar-Based	0:20	arg1	Testing					60:66	Thin-Layer-Agar-Based Direct Phenotypic Drug Susceptibility Testing	0:66	Thin-Layer-Agar-Based Direct Phenotypic Drug Susceptibility Testing on Sputum in Eswatini	0:88	Thin-Layer-Agar-Based Direct Phenotypic Drug Susceptibility Testing on Sputum in Eswatini Rapidly Detects Mycobacterium tuberculosis Growth and Rifampicin Resistance Otherwise Missed by WHO-Endorsed Diagnostic Tests.
33722892	9	34	theme	RR-confirmed	1460:1471	arg1	isolates					1473:1480	all RR-confirmed isolates	1456:1480	all RR-confirmed isolates	1456:1480	Eight isolates, 28.6% of all RR-confirmed isolates, carried the I491F mutation, all detected by TLA.
33722892	10	35	theme	specificity	1615:1625	arg1	%					1630:1630	99.5% specificity; 95% CI	1609:1633	99.5% specificity; 95% CI	1609:1633	TLA also correctly identified 183 of the 184 ofloxacin-susceptible isolates (99.5% specificity; 95% CI, 97.0 to 99.9).
33722892	5	36	theme	Löwenstein-Jensen	865:881	arg1	media					897:901	Löwenstein-Jensen or 7H11 solid media	865:901	media	897:901	TLA results were compared at the Reference Laboratory in Antwerp with indirect-DST on Löwenstein-Jensen or 7H11 solid media and additional phenotypic and genotypic testing to resolve discordance.
33722892	0	37	theme	Drug	40:43	arg1	Testing					60:66	Thin-Layer-Agar-Based Direct Phenotypic Drug Susceptibility Testing	0:66	Thin-Layer-Agar-Based Direct Phenotypic Drug Susceptibility Testing on Sputum in Eswatini	0:88	Thin-Layer-Agar-Based Direct Phenotypic Drug Susceptibility Testing on Sputum in Eswatini Rapidly Detects Mycobacterium tuberculosis Growth and Rifampicin Resistance Otherwise Missed by WHO-Endorsed Diagnostic Tests.
33722892	10	38	theme	isolates	1599:1606	arg1	isolates					1599:1606	the 184 ofloxacin-susceptible isolates	1569:1606	the 184 ofloxacin-susceptible isolates	1569:1606	TLA also correctly identified 183 of the 184 ofloxacin-susceptible isolates (99.5% specificity; 95% CI, 97.0 to 99.9).
33722892	10	38	theme	isolates	1599:1606	arg1	183					1562:1564	183	1562:1564	183	1562:1564	TLA also correctly identified 183 of the 184 ofloxacin-susceptible isolates (99.5% specificity; 95% CI, 97.0 to 99.9).
33722892	9	39	theme	isolates	1473:1480	arg1	isolates					1473:1480	all RR-confirmed isolates	1456:1480	all RR-confirmed isolates	1456:1480	Eight isolates, 28.6% of all RR-confirmed isolates, carried the I491F mutation, all detected by TLA.
33722892	9	39	theme	isolates	1473:1480	arg1	isolates					1437:1444	Eight isolates	1431:1444	Eight isolates	1431:1444	Eight isolates, 28.6% of all RR-confirmed isolates, carried the I491F mutation, all detected by TLA.
33722892	9	39	theme	isolates	1473:1480	arg1	%					1451:1451	28.6%	1447:1451	28.6% of all RR-confirmed isolates	1447:1480	Eight isolates, 28.6% of all RR-confirmed isolates, carried the I491F mutation, all detected by TLA.
33722892	2	40	theme	Mycobacterium	438:450	arg1	MTB					466:468	MTB	466:468	MTB	466:468	In addition, Xpert MTB/RIF does not distinguish between viable and dead Mycobacterium tuberculosis (MTB).
33722892	2	40	theme	Mycobacterium	438:450	arg1	tuberculosis					452:463	viable and dead Mycobacterium tuberculosis	422:463	viable and dead Mycobacterium tuberculosis (MTB)	422:469	In addition, Xpert MTB/RIF does not distinguish between viable and dead Mycobacterium tuberculosis (MTB).
33722892	0	41	theme	Phenotypic	29:38	arg1	Testing					60:66	Thin-Layer-Agar-Based Direct Phenotypic Drug Susceptibility Testing	0:66	Thin-Layer-Agar-Based Direct Phenotypic Drug Susceptibility Testing on Sputum in Eswatini	0:88	Thin-Layer-Agar-Based Direct Phenotypic Drug Susceptibility Testing on Sputum in Eswatini Rapidly Detects Mycobacterium tuberculosis Growth and Rifampicin Resistance Otherwise Missed by WHO-Endorsed Diagnostic Tests.
33722892	0	42	theme	Rifampicin	144:153	arg1	Resistance					155:164	Rifampicin Resistance	144:164	Rifampicin Resistance	144:164	Thin-Layer-Agar-Based Direct Phenotypic Drug Susceptibility Testing on Sputum in Eswatini Rapidly Detects Mycobacterium tuberculosis Growth and Rifampicin Resistance Otherwise Missed by WHO-Endorsed Diagnostic Tests.
33722892	9	43	contain	carried	1483:1489	arg2	all					1511:1513	all	1511:1513	all	1511:1513	Eight isolates, 28.6% of all RR-confirmed isolates, carried the I491F mutation, all detected by TLA.
33722892	9	43	contain	carried	1483:1489	arg2	mutation					1501:1508	the I491F mutation	1491:1508	the I491F mutation	1491:1508	Eight isolates, 28.6% of all RR-confirmed isolates, carried the I491F mutation, all detected by TLA.
33722892	9	43	contain	carried	1483:1489	arg1	isolates					1437:1444	Eight isolates	1431:1444	Eight isolates	1431:1444	Eight isolates, 28.6% of all RR-confirmed isolates, carried the I491F mutation, all detected by TLA.
33722892	9	43	contain	carried	1483:1489	arg1	%					1451:1451	28.6%	1447:1451	28.6% of all RR-confirmed isolates	1447:1480	Eight isolates, 28.6% of all RR-confirmed isolates, carried the I491F mutation, all detected by TLA.
33722892	9	43	contain	carried	1483:1489	arg1	isolates					1473:1480	all RR-confirmed isolates	1456:1480	all RR-confirmed isolates	1456:1480	Eight isolates, 28.6% of all RR-confirmed isolates, carried the I491F mutation, all detected by TLA.
33722892	8	44	theme	confidence	1258:1267	arg1	[CI					1278:1280	95% confidence interval [CI	1254:1280	95% confidence interval [CI	1254:1280	Within a median of 18.4 days, TLA detected RR with 93.0% sensitivity (95% confidence interval [CI], 77.4 to 98.0) and 99.4% specificity (95% CI, 96.7 to 99.9) versus 62.5% (95% CI, 42.7 to 78.8) and 99.3% (95% CI, 96.2 to 99.9) for Xpert MTB/RIF.
33722892	8	44	theme	confidence	1258:1267	arg1	RR					1227:1228	RR	1227:1228	RR	1227:1228	Within a median of 18.4 days, TLA detected RR with 93.0% sensitivity (95% confidence interval [CI], 77.4 to 98.0) and 99.4% specificity (95% CI, 96.7 to 99.9) versus 62.5% (95% CI, 42.7 to 78.8) and 99.3% (95% CI, 96.2 to 99.9) for Xpert MTB/RIF.
33722892	2	45	theme	dead	433:436	arg1	MTB					466:468	MTB	466:468	MTB	466:468	In addition, Xpert MTB/RIF does not distinguish between viable and dead Mycobacterium tuberculosis (MTB).
33722892	2	45	theme	dead	433:436	arg1	tuberculosis					452:463	viable and dead Mycobacterium tuberculosis	422:463	viable and dead Mycobacterium tuberculosis (MTB)	422:469	In addition, Xpert MTB/RIF does not distinguish between viable and dead Mycobacterium tuberculosis (MTB).
33722892	1	46	theme	rpoB	324:327	arg1	mutation					329:336	rpoB mutation	324:336	rpoB mutation	324:336	Xpert MTB/RIF rapidly detects resistance to rifampicin (RR); however, this test misses I491F-RR conferring rpoB mutation, common in southern Africa.
33722892	0	47	theme	Missed	176:181	arg1	Growth					133:138	Mycobacterium tuberculosis Growth	106:138	Mycobacterium tuberculosis Growth	106:138	Thin-Layer-Agar-Based Direct Phenotypic Drug Susceptibility Testing on Sputum in Eswatini Rapidly Detects Mycobacterium tuberculosis Growth and Rifampicin Resistance Otherwise Missed by WHO-Endorsed Diagnostic Tests.
33722892	8	48	theme	%	1256:1256	arg1	[CI					1278:1280	95% confidence interval [CI	1254:1280	95% confidence interval [CI	1254:1280	Within a median of 18.4 days, TLA detected RR with 93.0% sensitivity (95% confidence interval [CI], 77.4 to 98.0) and 99.4% specificity (95% CI, 96.7 to 99.9) versus 62.5% (95% CI, 42.7 to 78.8) and 99.3% (95% CI, 96.2 to 99.9) for Xpert MTB/RIF.
33722892	8	48	theme	%	1256:1256	arg1	RR					1227:1228	RR	1227:1228	RR	1227:1228	Within a median of 18.4 days, TLA detected RR with 93.0% sensitivity (95% confidence interval [CI], 77.4 to 98.0) and 99.4% specificity (95% CI, 96.7 to 99.9) versus 62.5% (95% CI, 42.7 to 78.8) and 99.3% (95% CI, 96.2 to 99.9) for Xpert MTB/RIF.
33722892	8	49	theme	18.4 days	1203:1211	arg1	median					1193:1198	a median	1191:1198	a median of 18.4 days	1191:1211	Within a median of 18.4 days, TLA detected RR with 93.0% sensitivity (95% confidence interval [CI], 77.4 to 98.0) and 99.4% specificity (95% CI, 96.7 to 99.9) versus 62.5% (95% CI, 42.7 to 78.8) and 99.3% (95% CI, 96.2 to 99.9) for Xpert MTB/RIF.
33722892	0	50	theme	Susceptibility	45:58	arg1	Testing					60:66	Thin-Layer-Agar-Based Direct Phenotypic Drug Susceptibility Testing	0:66	Thin-Layer-Agar-Based Direct Phenotypic Drug Susceptibility Testing on Sputum in Eswatini	0:88	Thin-Layer-Agar-Based Direct Phenotypic Drug Susceptibility Testing on Sputum in Eswatini Rapidly Detects Mycobacterium tuberculosis Growth and Rifampicin Resistance Otherwise Missed by WHO-Endorsed Diagnostic Tests.
33722892	10	51	dep	99.9	1644:1647	arg1	to					1641:1642	to	1641:1642	to	1641:1642	TLA also correctly identified 183 of the 184 ofloxacin-susceptible isolates (99.5% specificity; 95% CI, 97.0 to 99.9).
33722892	11	52	theme	RR	1768:1769	arg1	patients					1771:1778	additional RR patients	1757:1778	additional RR patients over Xpert MTB/RIF	1757:1797	In field conditions, TLA rapidly detects RR, and in this specific setting, it contributed to detection of additional RR patients over Xpert MTB/RIF, mainly but not exclusively due to I491F.
33722892	8	53	dep	%	1392:1392	arg1	99.9					1406:1409	99.9	1406:1409	99.9	1406:1409	Within a median of 18.4 days, TLA detected RR with 93.0% sensitivity (95% confidence interval [CI], 77.4 to 98.0) and 99.4% specificity (95% CI, 96.7 to 99.9) versus 62.5% (95% CI, 42.7 to 78.8) and 99.3% (95% CI, 96.2 to 99.9) for Xpert MTB/RIF.
33722892	8	54	dep	%	1323:1323	arg1	99.9					1337:1340	99.9	1337:1340	99.9	1337:1340	Within a median of 18.4 days, TLA detected RR with 93.0% sensitivity (95% confidence interval [CI], 77.4 to 98.0) and 99.4% specificity (95% CI, 96.7 to 99.9) versus 62.5% (95% CI, 42.7 to 78.8) and 99.3% (95% CI, 96.2 to 99.9) for Xpert MTB/RIF.
33722892	2	55	theme	viable	422:427	arg1	MTB					466:468	MTB	466:468	MTB	466:468	In addition, Xpert MTB/RIF does not distinguish between viable and dead Mycobacterium tuberculosis (MTB).
33722892	2	55	theme	viable	422:427	arg1	tuberculosis					452:463	viable and dead Mycobacterium tuberculosis	422:463	viable and dead Mycobacterium tuberculosis (MTB)	422:469	In addition, Xpert MTB/RIF does not distinguish between viable and dead Mycobacterium tuberculosis (MTB).
33722892	5	56	theme	TLA	779:781	arg1	results					783:789	TLA results	779:789	TLA results	779:789	TLA results were compared at the Reference Laboratory in Antwerp with indirect-DST on Löwenstein-Jensen or 7H11 solid media and additional phenotypic and genotypic testing to resolve discordance.
33722892	3	57	from	conditions	630:639	arg1	Eswatini					644:651	Eswatini	644:651	Eswatini	644:651	We aimed to investigate the ability of thin-layer agar (TLA) direct drug-susceptibility testing (DST) to detect MTB and its drug-resistance profiles in field conditions in Eswatini.
33722892	0	58	theme	WHO-Endorsed	186:197	arg1	Tests					210:214	WHO-Endorsed Diagnostic Tests	186:214	WHO-Endorsed Diagnostic Tests	186:214	Thin-Layer-Agar-Based Direct Phenotypic Drug Susceptibility Testing on Sputum in Eswatini Rapidly Detects Mycobacterium tuberculosis Growth and Rifampicin Resistance Otherwise Missed by WHO-Endorsed Diagnostic Tests.
33722892	10	59	theme	%	1613:1613	arg1	%					1630:1630	99.5% specificity; 95% CI	1609:1633	99.5% specificity; 95% CI	1609:1633	TLA also correctly identified 183 of the 184 ofloxacin-susceptible isolates (99.5% specificity; 95% CI, 97.0 to 99.9).
33722892	4	60	with	parallel	689:696	arg1	TLA					721:723	TLA	721:723	TLA	721:723	Consecutive samples were tested in parallel with Xpert MTB/RIF and TLA for rifampicin (1.0 μg/ml) and ofloxacin (2.0 μg/ml).
33722892	4	60	with	parallel	689:696	arg1	MTB/RIF					709:715	MTB/RIF	709:715	MTB/RIF	709:715	Consecutive samples were tested in parallel with Xpert MTB/RIF and TLA for rifampicin (1.0 μg/ml) and ofloxacin (2.0 μg/ml).
33722892	8	61	theme	%	1239:1239	arg1	sensitivity					1241:1251	93.0% sensitivity	1235:1251	93.0% sensitivity	1235:1251	Within a median of 18.4 days, TLA detected RR with 93.0% sensitivity (95% confidence interval [CI], 77.4 to 98.0) and 99.4% specificity (95% CI, 96.7 to 99.9) versus 62.5% (95% CI, 42.7 to 78.8) and 99.3% (95% CI, 96.2 to 99.9) for Xpert MTB/RIF.
33722892	1	62	from	Africa	358:363	arg1	common					339:344	common	339:344	common	339:344	Xpert MTB/RIF rapidly detects resistance to rifampicin (RR); however, this test misses I491F-RR conferring rpoB mutation, common in southern Africa.
33722892	1	63	theme	common	339:344	arg1	mutation					329:336	rpoB mutation	324:336	rpoB mutation	324:336	Xpert MTB/RIF rapidly detects resistance to rifampicin (RR); however, this test misses I491F-RR conferring rpoB mutation, common in southern Africa.
33722892	11	64	theme	patients	1771:1778	arg1	detection					1744:1752	detection	1744:1752	detection of additional RR patients over Xpert MTB/RIF	1744:1797	In field conditions, TLA rapidly detects RR, and in this specific setting, it contributed to detection of additional RR patients over Xpert MTB/RIF, mainly but not exclusively due to I491F.
33722892	7	65	theme	composite	1163:1171	arg1	reference					1173:1181	the composite reference	1159:1181	the composite reference	1159:1181	Of a total of 4,547 samples included in the study, 200 isolates were available for comparison to the composite reference.
33722892	5	66	theme	additional	907:916	arg1	testing					943:949	additional phenotypic and genotypic testing	907:949	additional phenotypic and genotypic testing	907:949	TLA results were compared at the Reference Laboratory in Antwerp with indirect-DST on Löwenstein-Jensen or 7H11 solid media and additional phenotypic and genotypic testing to resolve discordance.
33722892	11	67	theme	Xpert	1785:1789	arg1	MTB/RIF					1791:1797	Xpert MTB/RIF	1785:1797	Xpert MTB/RIF	1785:1797	In field conditions, TLA rapidly detects RR, and in this specific setting, it contributed to detection of additional RR patients over Xpert MTB/RIF, mainly but not exclusively due to I491F.
33722892	12	68	theme	fluoroquinolone	1870:1884	arg1	resistance					1886:1895	fluoroquinolone resistance	1870:1895	fluoroquinolone resistance	1870:1895	TLA also accurately excluded fluoroquinolone resistance.
33722892	5	69	from	Laboratory	822:831	arg1	Antwerp					836:842	Antwerp	836:842	Antwerp	836:842	TLA results were compared at the Reference Laboratory in Antwerp with indirect-DST on Löwenstein-Jensen or 7H11 solid media and additional phenotypic and genotypic testing to resolve discordance.
33722892	5	70	theme	phenotypic	918:927	arg1	testing					943:949	additional phenotypic and genotypic testing	907:949	additional phenotypic and genotypic testing	907:949	TLA results were compared at the Reference Laboratory in Antwerp with indirect-DST on Löwenstein-Jensen or 7H11 solid media and additional phenotypic and genotypic testing to resolve discordance.
33722892	0	71	theme	Diagnostic	199:208	arg1	Tests					210:214	WHO-Endorsed Diagnostic Tests	186:214	WHO-Endorsed Diagnostic Tests	186:214	Thin-Layer-Agar-Based Direct Phenotypic Drug Susceptibility Testing on Sputum in Eswatini Rapidly Detects Mycobacterium tuberculosis Growth and Rifampicin Resistance Otherwise Missed by WHO-Endorsed Diagnostic Tests.
33722892	10	72	theme	CI	1632:1633	arg1	%					1630:1630	99.5% specificity; 95% CI	1609:1633	99.5% specificity; 95% CI	1609:1633	TLA also correctly identified 183 of the 184 ofloxacin-susceptible isolates (99.5% specificity; 95% CI, 97.0 to 99.9).
33722892	3	73	theme	field	624:628	arg1	conditions					630:639	field conditions	624:639	field conditions in Eswatini	624:651	We aimed to investigate the ability of thin-layer agar (TLA) direct drug-susceptibility testing (DST) to detect MTB and its drug-resistance profiles in field conditions in Eswatini.
33722892	3	74	theme	TLA	528:530	arg1	DST					569:571	DST	569:571	DST	569:571	We aimed to investigate the ability of thin-layer agar (TLA) direct drug-susceptibility testing (DST) to detect MTB and its drug-resistance profiles in field conditions in Eswatini.
33722892	3	74	theme	TLA	528:530	arg1	testing					560:566	thin-layer agar (TLA) direct drug-susceptibility testing	511:566	thin-layer agar (TLA) direct drug-susceptibility testing (DST)	511:572	We aimed to investigate the ability of thin-layer agar (TLA) direct drug-susceptibility testing (DST) to detect MTB and its drug-resistance profiles in field conditions in Eswatini.
33722892	8	75	dep	78.8	1373:1376	arg1	to					1370:1371	to	1370:1371	to	1370:1371	Within a median of 18.4 days, TLA detected RR with 93.0% sensitivity (95% confidence interval [CI], 77.4 to 98.0) and 99.4% specificity (95% CI, 96.7 to 99.9) versus 62.5% (95% CI, 42.7 to 78.8) and 99.3% (95% CI, 96.2 to 99.9) for Xpert MTB/RIF.
33722892	6	76	theme	%	1028:1028	arg1	detection					1012:1020	MTB detection	1008:1020	MTB detection of 7.1% versus 10.0% for Xpert MTB/RIF	1008:1059	TLA showed a positivity rate for MTB detection of 7.1% versus 10.0% for Xpert MTB/RIF.
33722892	3	77	theme	drug-resistance	596:610	arg1	profiles					612:619	its drug-resistance profiles	592:619	its drug-resistance profiles	592:619	We aimed to investigate the ability of thin-layer agar (TLA) direct drug-susceptibility testing (DST) to detect MTB and its drug-resistance profiles in field conditions in Eswatini.
33722892	5	78	theme	Reference	812:820	arg1	Laboratory					822:831	the Reference Laboratory	808:831	the Reference Laboratory in Antwerp	808:842	TLA results were compared at the Reference Laboratory in Antwerp with indirect-DST on Löwenstein-Jensen or 7H11 solid media and additional phenotypic and genotypic testing to resolve discordance.
33722892	5	79	theme	7H11	886:889	arg1	media					897:901	Löwenstein-Jensen or 7H11 solid media	865:901	media	897:901	TLA results were compared at the Reference Laboratory in Antwerp with indirect-DST on Löwenstein-Jensen or 7H11 solid media and additional phenotypic and genotypic testing to resolve discordance.
33722892	4	80	theme	Consecutive	654:664	arg1	samples					666:672	Consecutive samples	654:672	Consecutive samples	654:672	Consecutive samples were tested in parallel with Xpert MTB/RIF and TLA for rifampicin (1.0 μg/ml) and ofloxacin (2.0 μg/ml).
33722892	8	81	dep	%	1359:1359	arg1	78.8					1373:1376	78.8	1373:1376	78.8	1373:1376	Within a median of 18.4 days, TLA detected RR with 93.0% sensitivity (95% confidence interval [CI], 77.4 to 98.0) and 99.4% specificity (95% CI, 96.7 to 99.9) versus 62.5% (95% CI, 42.7 to 78.8) and 99.3% (95% CI, 96.2 to 99.9) for Xpert MTB/RIF.
33722892	10	82	theme	ofloxacin-susceptible	1577:1597	arg1	isolates					1599:1606	the 184 ofloxacin-susceptible isolates	1569:1606	the 184 ofloxacin-susceptible isolates	1569:1606	TLA also correctly identified 183 of the 184 ofloxacin-susceptible isolates (99.5% specificity; 95% CI, 97.0 to 99.9).
33722892	2	83	theme	Xpert	379:383	arg1	MTB/RIF					385:391	Xpert MTB/RIF	379:391	Xpert MTB/RIF	379:391	In addition, Xpert MTB/RIF does not distinguish between viable and dead Mycobacterium tuberculosis (MTB).
33722892	6	84	theme	MTB	1008:1010	arg1	detection					1012:1020	MTB detection	1008:1020	MTB detection of 7.1% versus 10.0% for Xpert MTB/RIF	1008:1059	TLA showed a positivity rate for MTB detection of 7.1% versus 10.0% for Xpert MTB/RIF.
33149259	6	0	theme	PSA	886:888	arg1	N-glycans					890:898	PSA N-glycans	886:898	PSA N-glycans	886:898	PSA N-glycans were released, labelled and analysed by hydrophilic interaction liquid chromatography combined with exoglycosidase digestions.
33149259	3	1	theme	acid	520:523	arg1	percentage					494:503	the percentage	490:503	the percentage of α2,6-sialic acid (SA)	490:528	Aberrant glycosylation is involved in neoplastic progression and specific changes in PSA glycosylation pattern, as the reduction in the percentage of α2,6-sialic acid (SA) are associated with PCa aggressiveness.
33149259	7	2	from	glycoforms	1270:1279	arg1	PSA					1293:1295	standard PSA	1284:1295	standard PSA from healthy individuals	1284:1320	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	7	2	from	glycoforms	1270:1279	arg1	individuals					1310:1320	healthy individuals	1302:1320	healthy individuals	1302:1320	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	4	3	theme	main	611:614	arg1	glycoforms					631:640	the main sialylated PSA glycoforms	607:640	the main sialylated PSA glycoforms from blood serum of aggressive PCa patients	607:684	In this study, we have characterised the main sialylated PSA glycoforms from blood serum of aggressive PCa patients and have compared with those of standard PSA from healthy individuals' seminal plasma.
33149259	2	4	theme	prostate	235:242	arg1	pathologies					244:254	benign prostate pathologies	228:254	benign prostate pathologies	228:254	However, their lack of specificity to distinguish benign prostate pathologies from PCa, or indolent from aggressive PCa have prompted the study of new non-invasive PCa biomarkers.
33149259	1	5	theme	cancer	154:159	arg1	detection					167:175	prostate cancer (PCa) detection	145:175	prostate cancer (PCa) detection	145:175	Serum levels of prostate specific antigen (PSA) are commonly used for prostate cancer (PCa) detection.
33149259	4	6	theme	PSA	627:629	arg1	glycoforms					631:640	the main sialylated PSA glycoforms	607:640	the main sialylated PSA glycoforms from blood serum of aggressive PCa patients	607:684	In this study, we have characterised the main sialylated PSA glycoforms from blood serum of aggressive PCa patients and have compared with those of standard PSA from healthy individuals' seminal plasma.
33149259	5	7	theme	α2,3-SA	832:838	arg1	glycoforms					840:849	α2,3-SA glycoforms	832:849	α2,3-SA glycoforms using SNA affinity chromatography	832:883	PSA was immunoprecipitated and α2,6-SA were separated from α2,3-SA glycoforms using SNA affinity chromatography.
33149259	7	8	theme	sialylated	1067:1076	arg1	glycoforms					1078:1087	blood serum PSA sialylated glycoforms	1051:1087	blood serum PSA sialylated glycoforms containing GalNAc residues	1051:1114	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	3	9	theme	neoplastic	396:405	arg1	progression					407:417	neoplastic progression	396:417	neoplastic progression	396:417	Aberrant glycosylation is involved in neoplastic progression and specific changes in PSA glycosylation pattern, as the reduction in the percentage of α2,6-sialic acid (SA) are associated with PCa aggressiveness.
33149259	7	10	theme	serum	1057:1061	arg1	PSA					1063:1065	blood serum PSA	1051:1065	blood serum PSA sialylated glycoforms containing GalNAc residues	1051:1114	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	8	11	theme	specific	1462:1469	arg1	strategies					1471:1480	specific strategies to target them	1462:1495	specific strategies to target them	1462:1495	The identification of these main PSA glycoforms altered in aggressive PCa opens the way to design specific strategies to target them, which will be useful to improve PCa risk stratification.
33149259	7	12	with	structures	1221:1230	arg1	glycoforms					1270:1279	the major PSA glycoforms	1256:1279	the major PSA glycoforms in standard PSA from healthy individuals	1256:1320	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	7	12	with	structures	1221:1230	arg1	α2,6-SA					1237:1243	α2,6-SA	1237:1243	α2,6-SA	1237:1243	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	4	13	theme	aggressive	662:671	arg1	patients					677:684	aggressive PCa patients	662:684	aggressive PCa patients	662:684	In this study, we have characterised the main sialylated PSA glycoforms from blood serum of aggressive PCa patients and have compared with those of standard PSA from healthy individuals' seminal plasma.
33149259	4	14	theme	patients	677:684	arg1	serum					653:657	blood serum	647:657	blood serum of aggressive PCa patients	647:684	In this study, we have characterised the main sialylated PSA glycoforms from blood serum of aggressive PCa patients and have compared with those of standard PSA from healthy individuals' seminal plasma.
33149259	7	15	gly	sialylated	1067:1076	arg1	glycoforms					1078:1087	blood serum PSA sialylated glycoforms	1051:1087	blood serum PSA sialylated glycoforms containing GalNAc residues	1051:1114	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	0	16	theme	prostate	58:65	arg1	cancer					67:72	aggressive prostate cancer	47:72	aggressive prostate cancer	47:72	Characterisation of the main PSA glycoforms in aggressive prostate cancer.
33149259	2	17	theme	specificity	201:211	arg1	lack					193:196	their lack	187:196	their lack of specificity	187:211	However, their lack of specificity to distinguish benign prostate pathologies from PCa, or indolent from aggressive PCa have prompted the study of new non-invasive PCa biomarkers.
33149259	5	18	theme	affinity	861:868	arg1	chromatography					870:883	SNA affinity chromatography	857:883	SNA affinity chromatography	857:883	PSA was immunoprecipitated and α2,6-SA were separated from α2,3-SA glycoforms using SNA affinity chromatography.
33149259	0	19	from	Characterisation	0:15	arg1	cancer					67:72	aggressive prostate cancer	47:72	aggressive prostate cancer	47:72	Characterisation of the main PSA glycoforms in aggressive prostate cancer.
33149259	6	20	theme	interaction	952:962	arg1	chromatography					971:984	hydrophilic interaction liquid chromatography	940:984	hydrophilic interaction liquid chromatography combined with exoglycosidase digestions	940:1024	PSA N-glycans were released, labelled and analysed by hydrophilic interaction liquid chromatography combined with exoglycosidase digestions.
33149259	7	21	theme	biantennary	1209:1219	arg1	structures					1221:1230	the disialylated core fucosylated biantennary structures	1175:1230	the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals,	1175:1321	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	1	22	theme	Serum	75:79	arg1	levels					81:86	Serum levels	75:86	Serum levels of prostate specific antigen (PSA)	75:121	Serum levels of prostate specific antigen (PSA) are commonly used for prostate cancer (PCa) detection.
33149259	7	23	theme	aggressive	1348:1357	arg1	PCa					1359:1361	aggressive PCa	1348:1361	aggressive PCa	1348:1361	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	8	24	theme	PCa	1530:1532	arg1	stratification					1539:1552	PCa risk stratification	1530:1552	PCa risk stratification	1530:1552	The identification of these main PSA glycoforms altered in aggressive PCa opens the way to design specific strategies to target them, which will be useful to improve PCa risk stratification.
33149259	2	25	theme	PCa	342:344	arg1	biomarkers					346:355	new non-invasive PCa biomarkers	325:355	new non-invasive PCa biomarkers	325:355	However, their lack of specificity to distinguish benign prostate pathologies from PCa, or indolent from aggressive PCa have prompted the study of new non-invasive PCa biomarkers.
33149259	7	26	theme	core	1192:1195	arg1	structures					1221:1230	the disialylated core fucosylated biantennary structures	1175:1230	the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals,	1175:1321	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	8	27	theme	PSA	1397:1399	arg1	glycoforms					1401:1410	these main PSA glycoforms	1386:1410	these main PSA glycoforms altered in aggressive PCa	1386:1436	The identification of these main PSA glycoforms altered in aggressive PCa opens the way to design specific strategies to target them, which will be useful to improve PCa risk stratification.
33149259	1	28	theme	specific	100:107	arg1	PSA					118:120	PSA	118:120	PSA	118:120	Serum levels of prostate specific antigen (PSA) are commonly used for prostate cancer (PCa) detection.
33149259	1	28	theme	specific	100:107	arg1	antigen					109:115	prostate specific antigen	91:115	prostate specific antigen (PSA)	91:121	Serum levels of prostate specific antigen (PSA) are commonly used for prostate cancer (PCa) detection.
33149259	3	29	theme	specific	423:430	arg1	changes					432:438	specific changes	423:438	specific changes in PSA glycosylation pattern	423:467	Aberrant glycosylation is involved in neoplastic progression and specific changes in PSA glycosylation pattern, as the reduction in the percentage of α2,6-sialic acid (SA) are associated with PCa aggressiveness.
33149259	0	30	theme	PSA	29:31	arg1	glycoforms					33:42	the main PSA glycoforms	20:42	the main PSA glycoforms in aggressive prostate cancer	20:72	Characterisation of the main PSA glycoforms in aggressive prostate cancer.
33149259	3	31	theme	Aberrant	358:365	arg1	glycosylation					367:379	Aberrant glycosylation	358:379	Aberrant glycosylation	358:379	Aberrant glycosylation is involved in neoplastic progression and specific changes in PSA glycosylation pattern, as the reduction in the percentage of α2,6-sialic acid (SA) are associated with PCa aggressiveness.
33149259	8	32	theme	aggressive	1423:1432	arg1	PCa					1434:1436	aggressive PCa	1423:1436	aggressive PCa	1423:1436	The identification of these main PSA glycoforms altered in aggressive PCa opens the way to design specific strategies to target them, which will be useful to improve PCa risk stratification.
33149259	3	33	from	changes	432:438	arg1	pattern					461:467	PSA glycosylation pattern	443:467	PSA glycosylation pattern	443:467	Aberrant glycosylation is involved in neoplastic progression and specific changes in PSA glycosylation pattern, as the reduction in the percentage of α2,6-sialic acid (SA) are associated with PCa aggressiveness.
33149259	7	34	theme	PCa	1153:1155	arg1	patients					1157:1164	aggressive PCa patients	1142:1164	aggressive PCa patients	1142:1164	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	4	35	theme	healthy	736:742	arg1	individuals					744:754	healthy individuals	736:754	healthy individuals	736:754	In this study, we have characterised the main sialylated PSA glycoforms from blood serum of aggressive PCa patients and have compared with those of standard PSA from healthy individuals' seminal plasma.
33149259	8	36	theme	glycoforms	1401:1410	arg1	identification					1368:1381	The identification	1364:1381	The identification of these main PSA glycoforms altered in aggressive PCa	1364:1436	The identification of these main PSA glycoforms altered in aggressive PCa opens the way to design specific strategies to target them, which will be useful to improve PCa risk stratification.
33149259	3	37	theme	glycosylation	447:459	arg1	pattern					461:467	PSA glycosylation pattern	443:467	PSA glycosylation pattern	443:467	Aberrant glycosylation is involved in neoplastic progression and specific changes in PSA glycosylation pattern, as the reduction in the percentage of α2,6-sialic acid (SA) are associated with PCa aggressiveness.
33149259	8	38	gly	glycoforms	1401:1410	arg1	PSA					1397:1399	these main PSA glycoforms	1386:1410	these main PSA glycoforms altered in aggressive PCa	1386:1436	The identification of these main PSA glycoforms altered in aggressive PCa opens the way to design specific strategies to target them, which will be useful to improve PCa risk stratification.
33149259	0	39	gly	glycoforms	33:42	arg1	PSA					29:31	the main PSA glycoforms	20:42	the main PSA glycoforms in aggressive prostate cancer	20:72	Characterisation of the main PSA glycoforms in aggressive prostate cancer.
33149259	0	39	gly	glycoforms	33:42	arg2	cancer					67:72	aggressive prostate cancer	47:72	aggressive prostate cancer	47:72	Characterisation of the main PSA glycoforms in aggressive prostate cancer.
33149259	7	40	theme	standard	1284:1291	arg1	PSA					1293:1295	standard PSA	1284:1295	standard PSA from healthy individuals	1284:1320	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	2	41	theme	new	325:327	arg1	biomarkers					346:355	new non-invasive PCa biomarkers	325:355	new non-invasive PCa biomarkers	325:355	However, their lack of specificity to distinguish benign prostate pathologies from PCa, or indolent from aggressive PCa have prompted the study of new non-invasive PCa biomarkers.
33149259	0	42	theme	aggressive	47:56	arg1	cancer					67:72	aggressive prostate cancer	47:72	aggressive prostate cancer	47:72	Characterisation of the main PSA glycoforms in aggressive prostate cancer.
33149259	2	43	from	PCa	294:296	arg1	indolent					269:276	indolent	269:276	indolent	269:276	However, their lack of specificity to distinguish benign prostate pathologies from PCa, or indolent from aggressive PCa have prompted the study of new non-invasive PCa biomarkers.
33149259	7	44	theme	major	1260:1264	arg1	glycoforms					1270:1279	the major PSA glycoforms	1256:1279	the major PSA glycoforms in standard PSA from healthy individuals	1256:1320	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	7	44	theme	major	1260:1264	arg1	α2,6-SA					1237:1243	α2,6-SA	1237:1243	α2,6-SA	1237:1243	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	4	45	gly	sialylated	616:625	arg1	glycoforms					631:640	the main sialylated PSA glycoforms	607:640	the main sialylated PSA glycoforms from blood serum of aggressive PCa patients	607:684	In this study, we have characterised the main sialylated PSA glycoforms from blood serum of aggressive PCa patients and have compared with those of standard PSA from healthy individuals' seminal plasma.
33149259	3	46	from	reduction	477:485	arg1	percentage					494:503	the percentage	490:503	the percentage of α2,6-sialic acid (SA)	490:528	Aberrant glycosylation is involved in neoplastic progression and specific changes in PSA glycosylation pattern, as the reduction in the percentage of α2,6-sialic acid (SA) are associated with PCa aggressiveness.
33149259	3	47	theme	α2,6-sialic	508:518	arg1	SA					526:527	SA	526:527	SA	526:527	Aberrant glycosylation is involved in neoplastic progression and specific changes in PSA glycosylation pattern, as the reduction in the percentage of α2,6-sialic acid (SA) are associated with PCa aggressiveness.
33149259	3	47	theme	α2,6-sialic	508:518	arg1	acid					520:523	α2,6-sialic acid	508:523	α2,6-sialic acid (SA)	508:528	Aberrant glycosylation is involved in neoplastic progression and specific changes in PSA glycosylation pattern, as the reduction in the percentage of α2,6-sialic acid (SA) are associated with PCa aggressiveness.
33149259	1	48	used	used	136:139	arg2	levels					81:86	Serum levels	75:86	Serum levels of prostate specific antigen (PSA)	75:121	Serum levels of prostate specific antigen (PSA) are commonly used for prostate cancer (PCa) detection.
33149259	7	49	theme	GalNAc	1100:1105	arg1	residues					1107:1114	GalNAc residues	1100:1114	GalNAc residues	1100:1114	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	1	50	theme	prostate	145:152	arg1	PCa					162:164	PCa	162:164	PCa	162:164	Serum levels of prostate specific antigen (PSA) are commonly used for prostate cancer (PCa) detection.
33149259	1	50	theme	prostate	145:152	arg1	cancer					154:159	prostate cancer	145:159	prostate cancer (PCa) detection	145:175	Serum levels of prostate specific antigen (PSA) are commonly used for prostate cancer (PCa) detection.
33149259	6	51	theme	hydrophilic	940:950	arg1	chromatography					971:984	hydrophilic interaction liquid chromatography	940:984	hydrophilic interaction liquid chromatography combined with exoglycosidase digestions	940:1024	PSA N-glycans were released, labelled and analysed by hydrophilic interaction liquid chromatography combined with exoglycosidase digestions.
33149259	7	52	gly	glycoforms	1078:1087	arg1	PSA					1063:1065	blood serum PSA	1051:1065	blood serum PSA sialylated glycoforms containing GalNAc residues	1051:1114	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	7	53	dep	increased	1129:1137	arg1	whereas					1167:1173	whereas	1167:1173	whereas	1167:1173	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	7	54	theme	PSA	1063:1065	arg1	glycoforms					1078:1087	blood serum PSA sialylated glycoforms	1051:1087	blood serum PSA sialylated glycoforms containing GalNAc residues	1051:1114	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	4	55	dep	those	709:713	arg1	plasma					765:770	seminal plasma	757:770	those of standard PSA from healthy individuals' seminal plasma	709:770	In this study, we have characterised the main sialylated PSA glycoforms from blood serum of aggressive PCa patients and have compared with those of standard PSA from healthy individuals' seminal plasma.
33149259	7	56	theme	blood	1051:1055	arg1	PSA					1063:1065	blood serum PSA	1051:1065	blood serum PSA sialylated glycoforms containing GalNAc residues	1051:1114	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	3	57	theme	PCa	550:552	arg1	aggressiveness					554:567	PCa aggressiveness	550:567	PCa aggressiveness	550:567	Aberrant glycosylation is involved in neoplastic progression and specific changes in PSA glycosylation pattern, as the reduction in the percentage of α2,6-sialic acid (SA) are associated with PCa aggressiveness.
33149259	4	58	theme	sialylated	616:625	arg1	glycoforms					631:640	the main sialylated PSA glycoforms	607:640	the main sialylated PSA glycoforms from blood serum of aggressive PCa patients	607:684	In this study, we have characterised the main sialylated PSA glycoforms from blood serum of aggressive PCa patients and have compared with those of standard PSA from healthy individuals' seminal plasma.
33149259	0	59	from	cancer	67:72	arg1	Characterisation					0:15	Characterisation	0:15	Characterisation of the main PSA glycoforms in aggressive prostate cancer.	0:73	Characterisation of the main PSA glycoforms in aggressive prostate cancer.
33149259	4	60	gly	glycoforms	631:640	arg1	PSA					627:629	the main sialylated PSA glycoforms	607:640	the main sialylated PSA glycoforms from blood serum of aggressive PCa patients	607:684	In this study, we have characterised the main sialylated PSA glycoforms from blood serum of aggressive PCa patients and have compared with those of standard PSA from healthy individuals' seminal plasma.
33149259	1	61	theme	antigen	109:115	arg1	levels					81:86	Serum levels	75:86	Serum levels of prostate specific antigen (PSA)	75:121	Serum levels of prostate specific antigen (PSA) are commonly used for prostate cancer (PCa) detection.
33149259	5	62	gly	glycoforms	840:849	arg1	α2,3-SA					832:838	α2,3-SA glycoforms	832:849	α2,3-SA glycoforms using SNA affinity chromatography	832:883	PSA was immunoprecipitated and α2,6-SA were separated from α2,3-SA glycoforms using SNA affinity chromatography.
33149259	7	63	contain	containing	1089:1098	arg1	glycoforms					1078:1087	blood serum PSA sialylated glycoforms	1051:1087	blood serum PSA sialylated glycoforms containing GalNAc residues	1051:1114	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	7	63	contain	containing	1089:1098	arg2	residues					1107:1114	GalNAc residues	1100:1114	GalNAc residues	1100:1114	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	2	64	theme	benign	228:233	arg1	pathologies					244:254	benign prostate pathologies	228:254	benign prostate pathologies	228:254	However, their lack of specificity to distinguish benign prostate pathologies from PCa, or indolent from aggressive PCa have prompted the study of new non-invasive PCa biomarkers.
33149259	4	65	theme	PCa	673:675	arg1	patients					677:684	aggressive PCa patients	662:684	aggressive PCa patients	662:684	In this study, we have characterised the main sialylated PSA glycoforms from blood serum of aggressive PCa patients and have compared with those of standard PSA from healthy individuals' seminal plasma.
33149259	4	66	from	individuals	744:754	arg1	PSA					727:729	standard PSA	718:729	standard PSA from healthy individuals	718:754	In this study, we have characterised the main sialylated PSA glycoforms from blood serum of aggressive PCa patients and have compared with those of standard PSA from healthy individuals' seminal plasma.
33149259	6	67	theme	exoglycosidase	1000:1013	arg1	digestions					1015:1024	exoglycosidase digestions	1000:1024	exoglycosidase digestions	1000:1024	PSA N-glycans were released, labelled and analysed by hydrophilic interaction liquid chromatography combined with exoglycosidase digestions.
33149259	5	68	theme	SNA	857:859	arg1	chromatography					870:883	SNA affinity chromatography	857:883	SNA affinity chromatography	857:883	PSA was immunoprecipitated and α2,6-SA were separated from α2,3-SA glycoforms using SNA affinity chromatography.
33149259	4	69	theme	blood	647:651	arg1	serum					653:657	blood serum	647:657	blood serum of aggressive PCa patients	647:684	In this study, we have characterised the main sialylated PSA glycoforms from blood serum of aggressive PCa patients and have compared with those of standard PSA from healthy individuals' seminal plasma.
33149259	3	70	gly	glycosylation	367:379	arg1	progression					407:417	neoplastic progression	396:417	neoplastic progression	396:417	Aberrant glycosylation is involved in neoplastic progression and specific changes in PSA glycosylation pattern, as the reduction in the percentage of α2,6-sialic acid (SA) are associated with PCa aggressiveness.
33149259	3	70	gly	glycosylation	367:379	arg1	changes					432:438	specific changes	423:438	specific changes in PSA glycosylation pattern	423:467	Aberrant glycosylation is involved in neoplastic progression and specific changes in PSA glycosylation pattern, as the reduction in the percentage of α2,6-sialic acid (SA) are associated with PCa aggressiveness.
33149259	6	71	theme	liquid	964:969	arg1	chromatography					971:984	hydrophilic interaction liquid chromatography	940:984	hydrophilic interaction liquid chromatography combined with exoglycosidase digestions	940:1024	PSA N-glycans were released, labelled and analysed by hydrophilic interaction liquid chromatography combined with exoglycosidase digestions.
33149259	7	72	theme	fucosylated	1197:1207	arg1	structures					1221:1230	the disialylated core fucosylated biantennary structures	1175:1230	the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals,	1175:1321	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	0	73	from	glycoforms	33:42	arg1	cancer					67:72	aggressive prostate cancer	47:72	aggressive prostate cancer	47:72	Characterisation of the main PSA glycoforms in aggressive prostate cancer.
33149259	4	74	theme	standard	718:725	arg1	PSA					727:729	standard PSA	718:729	standard PSA from healthy individuals	718:754	In this study, we have characterised the main sialylated PSA glycoforms from blood serum of aggressive PCa patients and have compared with those of standard PSA from healthy individuals' seminal plasma.
33149259	2	75	theme	biomarkers	346:355	arg1	study					316:320	the study	312:320	the study of new non-invasive PCa biomarkers	312:355	However, their lack of specificity to distinguish benign prostate pathologies from PCa, or indolent from aggressive PCa have prompted the study of new non-invasive PCa biomarkers.
33149259	7	76	theme	disialylated	1179:1190	arg1	structures					1221:1230	the disialylated core fucosylated biantennary structures	1175:1230	the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals,	1175:1321	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	2	77	theme	non-invasive	329:340	arg1	biomarkers					346:355	new non-invasive PCa biomarkers	325:355	new non-invasive PCa biomarkers	325:355	However, their lack of specificity to distinguish benign prostate pathologies from PCa, or indolent from aggressive PCa have prompted the study of new non-invasive PCa biomarkers.
33149259	0	78	theme	main	24:27	arg1	glycoforms					33:42	the main PSA glycoforms	20:42	the main PSA glycoforms in aggressive prostate cancer	20:72	Characterisation of the main PSA glycoforms in aggressive prostate cancer.
33149259	7	79	gly	glycoforms	1270:1279	arg1	PSA					1266:1268	the major PSA glycoforms	1256:1279	the major PSA glycoforms in standard PSA from healthy individuals	1256:1320	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	7	79	gly	glycoforms	1270:1279	arg2	PSA					1293:1295	standard PSA	1284:1295	standard PSA from healthy individuals	1284:1320	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	1	80	theme	prostate	91:98	arg1	PSA					118:120	PSA	118:120	PSA	118:120	Serum levels of prostate specific antigen (PSA) are commonly used for prostate cancer (PCa) detection.
33149259	1	80	theme	prostate	91:98	arg1	antigen					109:115	prostate specific antigen	91:115	prostate specific antigen (PSA)	91:121	Serum levels of prostate specific antigen (PSA) are commonly used for prostate cancer (PCa) detection.
33149259	8	81	theme	main	1392:1395	arg1	glycoforms					1401:1410	these main PSA glycoforms	1386:1410	these main PSA glycoforms altered in aggressive PCa	1386:1436	The identification of these main PSA glycoforms altered in aggressive PCa opens the way to design specific strategies to target them, which will be useful to improve PCa risk stratification.
33149259	8	82	theme	risk	1534:1537	arg1	stratification					1539:1552	PCa risk stratification	1530:1552	PCa risk stratification	1530:1552	The identification of these main PSA glycoforms altered in aggressive PCa opens the way to design specific strategies to target them, which will be useful to improve PCa risk stratification.
33149259	3	83	theme	PSA	443:445	arg1	pattern					461:467	PSA glycosylation pattern	443:467	PSA glycosylation pattern	443:467	Aberrant glycosylation is involved in neoplastic progression and specific changes in PSA glycosylation pattern, as the reduction in the percentage of α2,6-sialic acid (SA) are associated with PCa aggressiveness.
33149259	0	84	theme	glycoforms	33:42	arg1	Characterisation					0:15	Characterisation	0:15	Characterisation of the main PSA glycoforms in aggressive prostate cancer.	0:73	Characterisation of the main PSA glycoforms in aggressive prostate cancer.
33149259	7	85	theme	healthy	1302:1308	arg1	individuals					1310:1320	healthy individuals	1302:1320	healthy individuals	1302:1320	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	4	86	theme	seminal	757:763	arg1	plasma					765:770	seminal plasma	757:770	those of standard PSA from healthy individuals' seminal plasma	709:770	In this study, we have characterised the main sialylated PSA glycoforms from blood serum of aggressive PCa patients and have compared with those of standard PSA from healthy individuals' seminal plasma.
33149259	7	87	theme	aggressive	1142:1151	arg1	patients					1157:1164	aggressive PCa patients	1142:1164	aggressive PCa patients	1142:1164	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	7	88	from	individuals	1310:1320	arg1	glycoforms					1270:1279	the major PSA glycoforms	1256:1279	the major PSA glycoforms in standard PSA from healthy individuals	1256:1320	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	7	88	from	individuals	1310:1320	arg1	PSA					1293:1295	standard PSA	1284:1295	standard PSA from healthy individuals	1284:1320	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	7	88	from	individuals	1310:1320	arg1	α2,6-SA					1237:1243	α2,6-SA	1237:1243	α2,6-SA	1237:1243	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	7	89	gly	fucosylated	1197:1207	arg1	structures					1221:1230	the disialylated core fucosylated biantennary structures	1175:1230	the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals,	1175:1321	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	7	90	gly	disialylated	1179:1190	arg1	structures					1221:1230	the disialylated core fucosylated biantennary structures	1175:1230	the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals,	1175:1321	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	2	91	theme	aggressive	283:292	arg1	PCa					294:296	aggressive PCa	283:296	aggressive PCa	283:296	However, their lack of specificity to distinguish benign prostate pathologies from PCa, or indolent from aggressive PCa have prompted the study of new non-invasive PCa biomarkers.
33149259	7	92	theme	PSA	1266:1268	arg1	glycoforms					1270:1279	the major PSA glycoforms	1256:1279	the major PSA glycoforms in standard PSA from healthy individuals	1256:1320	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	7	92	theme	PSA	1266:1268	arg1	α2,6-SA					1237:1243	α2,6-SA	1237:1243	α2,6-SA	1237:1243	The results showed that blood serum PSA sialylated glycoforms containing GalNAc residues were largely increased in aggressive PCa patients, whereas the disialylated core fucosylated biantennary structures with α2,6-SA, which are the major PSA glycoforms in standard PSA from healthy individuals, were markedly reduced in aggressive PCa.
33149259	3	93	from	progression	407:417	arg1	pattern					461:467	PSA glycosylation pattern	443:467	PSA glycosylation pattern	443:467	Aberrant glycosylation is involved in neoplastic progression and specific changes in PSA glycosylation pattern, as the reduction in the percentage of α2,6-sialic acid (SA) are associated with PCa aggressiveness.
33149259	4	94	from	serum	653:657	arg1	glycoforms					631:640	the main sialylated PSA glycoforms	607:640	the main sialylated PSA glycoforms from blood serum of aggressive PCa patients	607:684	In this study, we have characterised the main sialylated PSA glycoforms from blood serum of aggressive PCa patients and have compared with those of standard PSA from healthy individuals' seminal plasma.
34579036	5	0	theme	tissues	1126:1132	arg1	hyperlipidemia					1020:1033	hyperlipidemia	1020:1033	hyperlipidemia	1020:1033	The ASEC-based synbiotic intervention helped improve HFD-induced body weight gain, hyperlipidemia, impaired glucose tolerance, insulin resistance, and inflammation of the adipose and liver tissues.
34579036	5	0	theme	tissues	1126:1132	arg1	gain					1014:1017	HFD-induced body weight gain	990:1017	HFD-induced body weight gain	990:1017	The ASEC-based synbiotic intervention helped improve HFD-induced body weight gain, hyperlipidemia, impaired glucose tolerance, insulin resistance, and inflammation of the adipose and liver tissues.
34579036	5	0	theme	tissues	1126:1132	arg1	tolerance					1053:1061	impaired glucose tolerance	1036:1061	impaired glucose tolerance	1036:1061	The ASEC-based synbiotic intervention helped improve HFD-induced body weight gain, hyperlipidemia, impaired glucose tolerance, insulin resistance, and inflammation of the adipose and liver tissues.
34579036	5	0	theme	tissues	1126:1132	arg1	resistance					1072:1081	insulin resistance	1064:1081	insulin resistance	1064:1081	The ASEC-based synbiotic intervention helped improve HFD-induced body weight gain, hyperlipidemia, impaired glucose tolerance, insulin resistance, and inflammation of the adipose and liver tissues.
34579036	5	0	theme	tissues	1126:1132	arg1	inflammation					1088:1099	inflammation	1088:1099	inflammation	1088:1099	The ASEC-based synbiotic intervention helped improve HFD-induced body weight gain, hyperlipidemia, impaired glucose tolerance, insulin resistance, and inflammation of the adipose and liver tissues.
34579036	4	1	theme	intervention	702:713	arg1	effects					673:679	the effects	669:679	the effects of a novel synbiotic intervention, consisting of an adlay seed extrusion cooked (ASEC)-based prebiotic and probiotic (Lactobacillus paracasei and Bacillus coagulans) on metabolic disorders and microbial dysbiosis in high-fat diet (HFD)-induced obese mice	669:934	Here, we investigated the effects of a novel synbiotic intervention, consisting of an adlay seed extrusion cooked (ASEC)-based prebiotic and probiotic (Lactobacillus paracasei and Bacillus coagulans) on metabolic disorders and microbial dysbiosis in high-fat diet (HFD)-induced obese mice.
34579036	7	2	from	families	1501:1508	arg1	mice					1591:1594	HFD-induced obese mice	1573:1594	HFD-induced obese mice	1573:1594	In particular, the ASEC-based synbiotic intervention increased the relative abundance of families Ruminococcaceae and Muribaculaceae and order Bacteroidales and reduced that of families Lactobacillaceae, Erysipelotrichaceae, and Streptococcaceae in HFD-induced obese mice.
34579036	7	3	dep	families	1413:1420	arg1	families					1413:1420	families	1413:1420	families Ruminococcaceae and Muribaculaceae	1413:1455	In particular, the ASEC-based synbiotic intervention increased the relative abundance of families Ruminococcaceae and Muribaculaceae and order Bacteroidales and reduced that of families Lactobacillaceae, Erysipelotrichaceae, and Streptococcaceae in HFD-induced obese mice.
34579036	7	3	dep	families	1413:1420	arg1	Muribaculaceae					1442:1455	Muribaculaceae	1442:1455	Muribaculaceae	1442:1455	In particular, the ASEC-based synbiotic intervention increased the relative abundance of families Ruminococcaceae and Muribaculaceae and order Bacteroidales and reduced that of families Lactobacillaceae, Erysipelotrichaceae, and Streptococcaceae in HFD-induced obese mice.
34579036	7	3	dep	families	1413:1420	arg1	Ruminococcaceae					1422:1436	Ruminococcaceae	1422:1436	Ruminococcaceae	1422:1436	In particular, the ASEC-based synbiotic intervention increased the relative abundance of families Ruminococcaceae and Muribaculaceae and order Bacteroidales and reduced that of families Lactobacillaceae, Erysipelotrichaceae, and Streptococcaceae in HFD-induced obese mice.
34579036	0	4	theme	Metabolic	90:98	arg1	Disturbance					100:110	Diet-Induced Metabolic Disturbance	77:110	Diet-Induced Metabolic Disturbance	77:110	Synbiotic Intervention with an Adlay-Based Prebiotic and Probiotics Improved Diet-Induced Metabolic Disturbance in Mice by Modulation of the Gut Microbiota.
34579036	6	5	theme	diversity	1271:1279	arg1	reconstitution					1239:1252	reconstitution	1239:1252	reconstitution of gut bacterial diversity and composition in HFD-induced obese mice	1239:1321	In addition, data from fecal metagenomics indicated that the ASEC-based synbiotic intervention fostered reconstitution of gut bacterial diversity and composition in HFD-induced obese mice.
34579036	4	6	theme	synbiotic	692:700	arg1	intervention					702:713	a novel synbiotic intervention	684:713	a novel synbiotic intervention	684:713	Here, we investigated the effects of a novel synbiotic intervention, consisting of an adlay seed extrusion cooked (ASEC)-based prebiotic and probiotic (Lactobacillus paracasei and Bacillus coagulans) on metabolic disorders and microbial dysbiosis in high-fat diet (HFD)-induced obese mice.
34579036	4	7	from	disorders	860:868	arg1	mice					931:934	high-fat diet (HFD)-induced obese mice	897:934	high-fat diet (HFD)-induced obese mice	897:934	Here, we investigated the effects of a novel synbiotic intervention, consisting of an adlay seed extrusion cooked (ASEC)-based prebiotic and probiotic (Lactobacillus paracasei and Bacillus coagulans) on metabolic disorders and microbial dysbiosis in high-fat diet (HFD)-induced obese mice.
34579036	6	8	theme	HFD-induced	1300:1310	arg1	mice					1318:1321	HFD-induced obese mice	1300:1321	HFD-induced obese mice	1300:1321	In addition, data from fecal metagenomics indicated that the ASEC-based synbiotic intervention fostered reconstitution of gut bacterial diversity and composition in HFD-induced obese mice.
34579036	4	9	theme	ASEC	762:765	arg1	prebiotic					774:782	an adlay seed extrusion cooked (ASEC)-based prebiotic	730:782	an adlay seed extrusion cooked (ASEC)-based prebiotic	730:782	Here, we investigated the effects of a novel synbiotic intervention, consisting of an adlay seed extrusion cooked (ASEC)-based prebiotic and probiotic (Lactobacillus paracasei and Bacillus coagulans) on metabolic disorders and microbial dysbiosis in high-fat diet (HFD)-induced obese mice.
34579036	5	10	theme	ASEC-based	941:950	arg1	intervention					962:973	The ASEC-based synbiotic intervention	937:973	The ASEC-based synbiotic intervention	937:973	The ASEC-based synbiotic intervention helped improve HFD-induced body weight gain, hyperlipidemia, impaired glucose tolerance, insulin resistance, and inflammation of the adipose and liver tissues.
34579036	7	11	theme	order	1461:1465	arg1	Bacteroidales					1467:1479	order Bacteroidales	1461:1479	order Bacteroidales	1461:1479	In particular, the ASEC-based synbiotic intervention increased the relative abundance of families Ruminococcaceae and Muribaculaceae and order Bacteroidales and reduced that of families Lactobacillaceae, Erysipelotrichaceae, and Streptococcaceae in HFD-induced obese mice.
34579036	1	12	from	issue	286:290	arg1	societies					302:310	modern societies	295:310	modern societies	295:310	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	6	13	from	metagenomics	1164:1175	arg1	data					1148:1151	data	1148:1151	data from fecal metagenomics	1148:1175	In addition, data from fecal metagenomics indicated that the ASEC-based synbiotic intervention fostered reconstitution of gut bacterial diversity and composition in HFD-induced obese mice.
34579036	8	14	theme	metabolic	1734:1742	arg1	disorders					1744:1752	metabolic disorders	1734:1752	metabolic disorders	1734:1752	Collectively, our results suggest that delayed dietary intervention with the novel ASEC-based synbiotic ameliorates HFD-induced obesity, metabolic disorders, and dysbiosis.
34579036	6	15	theme	composition	1285:1295	arg1	reconstitution					1239:1252	reconstitution	1239:1252	reconstitution of gut bacterial diversity and composition in HFD-induced obese mice	1239:1321	In addition, data from fecal metagenomics indicated that the ASEC-based synbiotic intervention fostered reconstitution of gut bacterial diversity and composition in HFD-induced obese mice.
34579036	7	16	theme	Bacteroidales	1467:1479	arg1	abundance					1400:1408	the relative abundance	1387:1408	the relative abundance of families Ruminococcaceae and Muribaculaceae and order Bacteroidales	1387:1479	In particular, the ASEC-based synbiotic intervention increased the relative abundance of families Ruminococcaceae and Muribaculaceae and order Bacteroidales and reduced that of families Lactobacillaceae, Erysipelotrichaceae, and Streptococcaceae in HFD-induced obese mice.
34579036	4	17	theme	-based	767:772	arg1	prebiotic					774:782	an adlay seed extrusion cooked (ASEC)-based prebiotic	730:782	an adlay seed extrusion cooked (ASEC)-based prebiotic	730:782	Here, we investigated the effects of a novel synbiotic intervention, consisting of an adlay seed extrusion cooked (ASEC)-based prebiotic and probiotic (Lactobacillus paracasei and Bacillus coagulans) on metabolic disorders and microbial dysbiosis in high-fat diet (HFD)-induced obese mice.
34579036	4	18	from	effects	673:679	arg1	disorders					860:868	metabolic disorders	850:868	metabolic disorders	850:868	Here, we investigated the effects of a novel synbiotic intervention, consisting of an adlay seed extrusion cooked (ASEC)-based prebiotic and probiotic (Lactobacillus paracasei and Bacillus coagulans) on metabolic disorders and microbial dysbiosis in high-fat diet (HFD)-induced obese mice.
34579036	4	18	from	effects	673:679	arg1	dysbiosis					884:892	microbial dysbiosis	874:892	microbial dysbiosis	874:892	Here, we investigated the effects of a novel synbiotic intervention, consisting of an adlay seed extrusion cooked (ASEC)-based prebiotic and probiotic (Lactobacillus paracasei and Bacillus coagulans) on metabolic disorders and microbial dysbiosis in high-fat diet (HFD)-induced obese mice.
34579036	6	19	theme	synbiotic	1207:1215	arg1	intervention					1217:1228	the ASEC-based synbiotic intervention	1192:1228	the ASEC-based synbiotic intervention	1192:1228	In addition, data from fecal metagenomics indicated that the ASEC-based synbiotic intervention fostered reconstitution of gut bacterial diversity and composition in HFD-induced obese mice.
34579036	5	20	theme	adipose	1108:1114	arg1	tissues					1126:1132	the adipose and liver tissues	1104:1132	tissues	1126:1132	The ASEC-based synbiotic intervention helped improve HFD-induced body weight gain, hyperlipidemia, impaired glucose tolerance, insulin resistance, and inflammation of the adipose and liver tissues.
34579036	6	21	theme	fecal	1158:1162	arg1	metagenomics					1164:1175	fecal metagenomics	1158:1175	fecal metagenomics	1158:1175	In addition, data from fecal metagenomics indicated that the ASEC-based synbiotic intervention fostered reconstitution of gut bacterial diversity and composition in HFD-induced obese mice.
34579036	4	22	theme	obese	925:929	arg1	mice					931:934	high-fat diet (HFD)-induced obese mice	897:934	high-fat diet (HFD)-induced obese mice	897:934	Here, we investigated the effects of a novel synbiotic intervention, consisting of an adlay seed extrusion cooked (ASEC)-based prebiotic and probiotic (Lactobacillus paracasei and Bacillus coagulans) on metabolic disorders and microbial dysbiosis in high-fat diet (HFD)-induced obese mice.
34579036	1	23	theme	associated	184:193	arg1	conditions					195:204	its associated conditions	180:204	its associated conditions	180:204	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	1	23	theme	associated	184:193	arg1	issue					286:290	a major public health issue	264:290	a major public health issue in modern societies	264:310	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	1	23	theme	associated	184:193	arg1	mellitus					243:250	type 2 diabetes mellitus	227:250	type 2 diabetes mellitus (T2DM)	227:257	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	1	23	theme	associated	184:193	arg1	syndrome					167:174	Metabolic syndrome	157:174	Metabolic syndrome	157:174	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	1	23	theme	associated	184:193	arg1	obesity					215:221	obesity	215:221	obesity	215:221	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	2	24	theme	associated	463:472	arg1	comorbidities					474:486	associated comorbidities	463:486	associated comorbidities	463:486	Dietary interventions, including microbiota-directed foods which effectively modulate the gut microbiome, may influence the regulation of obesity and associated comorbidities.
34579036	8	25	theme	delayed	1636:1642	arg1	intervention					1652:1663	delayed dietary intervention	1636:1663	delayed dietary intervention with the novel ASEC-based synbiotic	1636:1699	Collectively, our results suggest that delayed dietary intervention with the novel ASEC-based synbiotic ameliorates HFD-induced obesity, metabolic disorders, and dysbiosis.
34579036	4	26	theme	metabolic	850:858	arg1	disorders					860:868	metabolic disorders	850:868	metabolic disorders	850:868	Here, we investigated the effects of a novel synbiotic intervention, consisting of an adlay seed extrusion cooked (ASEC)-based prebiotic and probiotic (Lactobacillus paracasei and Bacillus coagulans) on metabolic disorders and microbial dysbiosis in high-fat diet (HFD)-induced obese mice.
34579036	4	27	theme	seed	739:742	arg1	prebiotic					774:782	an adlay seed extrusion cooked (ASEC)-based prebiotic	730:782	an adlay seed extrusion cooked (ASEC)-based prebiotic	730:782	Here, we investigated the effects of a novel synbiotic intervention, consisting of an adlay seed extrusion cooked (ASEC)-based prebiotic and probiotic (Lactobacillus paracasei and Bacillus coagulans) on metabolic disorders and microbial dysbiosis in high-fat diet (HFD)-induced obese mice.
34579036	0	28	theme	Synbiotic	0:8	arg1	Intervention					10:21	Synbiotic Intervention	0:21	Synbiotic Intervention with an Adlay-Based Prebiotic and Probiotics	0:66	Synbiotic Intervention with an Adlay-Based Prebiotic and Probiotics Improved Diet-Induced Metabolic Disturbance in Mice by Modulation of the Gut Microbiota.
34579036	6	29	theme	ASEC-based	1196:1205	arg1	intervention					1217:1228	the ASEC-based synbiotic intervention	1192:1228	the ASEC-based synbiotic intervention	1192:1228	In addition, data from fecal metagenomics indicated that the ASEC-based synbiotic intervention fostered reconstitution of gut bacterial diversity and composition in HFD-induced obese mice.
34579036	6	30	theme	bacterial	1261:1269	arg1	diversity					1271:1279	gut bacterial diversity	1257:1279	gut bacterial diversity	1257:1279	In addition, data from fecal metagenomics indicated that the ASEC-based synbiotic intervention fostered reconstitution of gut bacterial diversity and composition in HFD-induced obese mice.
34579036	3	31	from	research	498:505	arg1	prebiotics					525:534	prebiotics	525:534	prebiotics	525:534	Although research on probiotics and prebiotics has been conducted extensively in recent years, diets with the use of synbiotics remain relatively unexplored.
34579036	3	31	from	research	498:505	arg1	probiotics					510:519	probiotics	510:519	probiotics	510:519	Although research on probiotics and prebiotics has been conducted extensively in recent years, diets with the use of synbiotics remain relatively unexplored.
34579036	4	32	theme	adlay	733:737	arg1	prebiotic					774:782	an adlay seed extrusion cooked (ASEC)-based prebiotic	730:782	an adlay seed extrusion cooked (ASEC)-based prebiotic	730:782	Here, we investigated the effects of a novel synbiotic intervention, consisting of an adlay seed extrusion cooked (ASEC)-based prebiotic and probiotic (Lactobacillus paracasei and Bacillus coagulans) on metabolic disorders and microbial dysbiosis in high-fat diet (HFD)-induced obese mice.
34579036	6	33	theme	obese	1312:1316	arg1	mice					1318:1321	HFD-induced obese mice	1300:1321	HFD-induced obese mice	1300:1321	In addition, data from fecal metagenomics indicated that the ASEC-based synbiotic intervention fostered reconstitution of gut bacterial diversity and composition in HFD-induced obese mice.
34579036	5	34	theme	weight	1007:1012	arg1	gain					1014:1017	HFD-induced body weight gain	990:1017	HFD-induced body weight gain	990:1017	The ASEC-based synbiotic intervention helped improve HFD-induced body weight gain, hyperlipidemia, impaired glucose tolerance, insulin resistance, and inflammation of the adipose and liver tissues.
34579036	7	35	theme	families	1413:1420	arg1	abundance					1400:1408	the relative abundance	1387:1408	the relative abundance of families Ruminococcaceae and Muribaculaceae and order Bacteroidales	1387:1479	In particular, the ASEC-based synbiotic intervention increased the relative abundance of families Ruminococcaceae and Muribaculaceae and order Bacteroidales and reduced that of families Lactobacillaceae, Erysipelotrichaceae, and Streptococcaceae in HFD-induced obese mice.
34579036	3	36	theme	recent	570:575	arg1	years					577:581	recent years	570:581	recent years	570:581	Although research on probiotics and prebiotics has been conducted extensively in recent years, diets with the use of synbiotics remain relatively unexplored.
34579036	5	37	theme	synbiotic	952:960	arg1	intervention					962:973	The ASEC-based synbiotic intervention	937:973	The ASEC-based synbiotic intervention	937:973	The ASEC-based synbiotic intervention helped improve HFD-induced body weight gain, hyperlipidemia, impaired glucose tolerance, insulin resistance, and inflammation of the adipose and liver tissues.
34579036	3	38	with	diets	584:588	arg1	use					599:601	the use	595:601	the use of synbiotics	595:615	Although research on probiotics and prebiotics has been conducted extensively in recent years, diets with the use of synbiotics remain relatively unexplored.
34579036	1	39	theme	major	266:270	arg1	conditions					195:204	its associated conditions	180:204	its associated conditions	180:204	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	1	39	theme	major	266:270	arg1	issue					286:290	a major public health issue	264:290	a major public health issue in modern societies	264:310	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	1	39	theme	major	266:270	arg1	mellitus					243:250	type 2 diabetes mellitus	227:250	type 2 diabetes mellitus (T2DM)	227:257	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	1	39	theme	major	266:270	arg1	syndrome					167:174	Metabolic syndrome	157:174	Metabolic syndrome	157:174	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	1	39	theme	major	266:270	arg1	obesity					215:221	obesity	215:221	obesity	215:221	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	4	40	theme	-induced	916:923	arg1	mice					931:934	high-fat diet (HFD)-induced obese mice	897:934	high-fat diet (HFD)-induced obese mice	897:934	Here, we investigated the effects of a novel synbiotic intervention, consisting of an adlay seed extrusion cooked (ASEC)-based prebiotic and probiotic (Lactobacillus paracasei and Bacillus coagulans) on metabolic disorders and microbial dysbiosis in high-fat diet (HFD)-induced obese mice.
34579036	4	41	theme	extrusion	744:752	arg1	prebiotic					774:782	an adlay seed extrusion cooked (ASEC)-based prebiotic	730:782	an adlay seed extrusion cooked (ASEC)-based prebiotic	730:782	Here, we investigated the effects of a novel synbiotic intervention, consisting of an adlay seed extrusion cooked (ASEC)-based prebiotic and probiotic (Lactobacillus paracasei and Bacillus coagulans) on metabolic disorders and microbial dysbiosis in high-fat diet (HFD)-induced obese mice.
34579036	8	42	theme	dietary	1644:1650	arg1	intervention					1652:1663	delayed dietary intervention	1636:1663	delayed dietary intervention with the novel ASEC-based synbiotic	1636:1699	Collectively, our results suggest that delayed dietary intervention with the novel ASEC-based synbiotic ameliorates HFD-induced obesity, metabolic disorders, and dysbiosis.
34579036	2	43	theme	comorbidities	474:486	arg1	regulation					437:446	the regulation	433:446	the regulation of obesity and associated comorbidities	433:486	Dietary interventions, including microbiota-directed foods which effectively modulate the gut microbiome, may influence the regulation of obesity and associated comorbidities.
34579036	1	44	theme	public	272:277	arg1	conditions					195:204	its associated conditions	180:204	its associated conditions	180:204	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	1	44	theme	public	272:277	arg1	issue					286:290	a major public health issue	264:290	a major public health issue in modern societies	264:310	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	1	44	theme	public	272:277	arg1	mellitus					243:250	type 2 diabetes mellitus	227:250	type 2 diabetes mellitus (T2DM)	227:257	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	1	44	theme	public	272:277	arg1	syndrome					167:174	Metabolic syndrome	157:174	Metabolic syndrome	157:174	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	1	44	theme	public	272:277	arg1	obesity					215:221	obesity	215:221	obesity	215:221	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	0	45	theme	Microbiota	145:154	arg1	Modulation					123:132	Modulation	123:132	Modulation of the Gut Microbiota	123:154	Synbiotic Intervention with an Adlay-Based Prebiotic and Probiotics Improved Diet-Induced Metabolic Disturbance in Mice by Modulation of the Gut Microbiota.
34579036	6	46	theme	gut	1257:1259	arg1	diversity					1271:1279	gut bacterial diversity	1257:1279	gut bacterial diversity	1257:1279	In addition, data from fecal metagenomics indicated that the ASEC-based synbiotic intervention fostered reconstitution of gut bacterial diversity and composition in HFD-induced obese mice.
34579036	7	47	theme	ASEC-based	1343:1352	arg1	intervention					1364:1375	the ASEC-based synbiotic intervention	1339:1375	the ASEC-based synbiotic intervention	1339:1375	In particular, the ASEC-based synbiotic intervention increased the relative abundance of families Ruminococcaceae and Muribaculaceae and order Bacteroidales and reduced that of families Lactobacillaceae, Erysipelotrichaceae, and Streptococcaceae in HFD-induced obese mice.
34579036	0	48	theme	Adlay-Based	31:41	arg1	Prebiotic					43:51	an Adlay-Based Prebiotic	28:51	an Adlay-Based Prebiotic	28:51	Synbiotic Intervention with an Adlay-Based Prebiotic and Probiotics Improved Diet-Induced Metabolic Disturbance in Mice by Modulation of the Gut Microbiota.
34579036	1	49	theme	health	279:284	arg1	conditions					195:204	its associated conditions	180:204	its associated conditions	180:204	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	1	49	theme	health	279:284	arg1	issue					286:290	a major public health issue	264:290	a major public health issue in modern societies	264:310	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	1	49	theme	health	279:284	arg1	mellitus					243:250	type 2 diabetes mellitus	227:250	type 2 diabetes mellitus (T2DM)	227:257	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	1	49	theme	health	279:284	arg1	syndrome					167:174	Metabolic syndrome	157:174	Metabolic syndrome	157:174	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	1	49	theme	health	279:284	arg1	obesity					215:221	obesity	215:221	obesity	215:221	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	0	50	theme	Gut	141:143	arg1	Microbiota					145:154	the Gut Microbiota	137:154	the Gut Microbiota	137:154	Synbiotic Intervention with an Adlay-Based Prebiotic and Probiotics Improved Diet-Induced Metabolic Disturbance in Mice by Modulation of the Gut Microbiota.
34579036	7	51	theme	synbiotic	1354:1362	arg1	intervention					1364:1375	the ASEC-based synbiotic intervention	1339:1375	the ASEC-based synbiotic intervention	1339:1375	In particular, the ASEC-based synbiotic intervention increased the relative abundance of families Ruminococcaceae and Muribaculaceae and order Bacteroidales and reduced that of families Lactobacillaceae, Erysipelotrichaceae, and Streptococcaceae in HFD-induced obese mice.
34579036	8	52	theme	HFD-induced	1713:1723	arg1	obesity					1725:1731	HFD-induced obesity	1713:1731	HFD-induced obesity	1713:1731	Collectively, our results suggest that delayed dietary intervention with the novel ASEC-based synbiotic ameliorates HFD-induced obesity, metabolic disorders, and dysbiosis.
34579036	2	53	theme	gut	403:405	arg1	microbiome					407:416	the gut microbiome	399:416	the gut microbiome	399:416	Dietary interventions, including microbiota-directed foods which effectively modulate the gut microbiome, may influence the regulation of obesity and associated comorbidities.
34579036	7	54	dep	families	1501:1508	arg1	families					1501:1508	families Lactobacillaceae, Erysipelotrichaceae, and Streptococcaceae	1501:1568	families Lactobacillaceae, Erysipelotrichaceae, and Streptococcaceae in HFD-induced obese mice	1501:1594	In particular, the ASEC-based synbiotic intervention increased the relative abundance of families Ruminococcaceae and Muribaculaceae and order Bacteroidales and reduced that of families Lactobacillaceae, Erysipelotrichaceae, and Streptococcaceae in HFD-induced obese mice.
34579036	7	54	dep	families	1501:1508	arg1	Erysipelotrichaceae					1528:1546	Erysipelotrichaceae	1528:1546	Erysipelotrichaceae	1528:1546	In particular, the ASEC-based synbiotic intervention increased the relative abundance of families Ruminococcaceae and Muribaculaceae and order Bacteroidales and reduced that of families Lactobacillaceae, Erysipelotrichaceae, and Streptococcaceae in HFD-induced obese mice.
34579036	7	54	dep	families	1501:1508	arg1	Streptococcaceae					1553:1568	Streptococcaceae	1553:1568	Streptococcaceae	1553:1568	In particular, the ASEC-based synbiotic intervention increased the relative abundance of families Ruminococcaceae and Muribaculaceae and order Bacteroidales and reduced that of families Lactobacillaceae, Erysipelotrichaceae, and Streptococcaceae in HFD-induced obese mice.
34579036	7	54	dep	families	1501:1508	arg1	Lactobacillaceae					1510:1525	Lactobacillaceae	1510:1525	Lactobacillaceae	1510:1525	In particular, the ASEC-based synbiotic intervention increased the relative abundance of families Ruminococcaceae and Muribaculaceae and order Bacteroidales and reduced that of families Lactobacillaceae, Erysipelotrichaceae, and Streptococcaceae in HFD-induced obese mice.
34579036	4	55	theme	high-fat	897:904	arg1	diet					906:909	high-fat diet	897:909	high-fat diet (HFD)-induced obese mice	897:934	Here, we investigated the effects of a novel synbiotic intervention, consisting of an adlay seed extrusion cooked (ASEC)-based prebiotic and probiotic (Lactobacillus paracasei and Bacillus coagulans) on metabolic disorders and microbial dysbiosis in high-fat diet (HFD)-induced obese mice.
34579036	4	55	theme	high-fat	897:904	arg1	HFD					912:914	HFD	912:914	HFD	912:914	Here, we investigated the effects of a novel synbiotic intervention, consisting of an adlay seed extrusion cooked (ASEC)-based prebiotic and probiotic (Lactobacillus paracasei and Bacillus coagulans) on metabolic disorders and microbial dysbiosis in high-fat diet (HFD)-induced obese mice.
34579036	2	56	theme	obesity	451:457	arg1	regulation					437:446	the regulation	433:446	the regulation of obesity and associated comorbidities	433:486	Dietary interventions, including microbiota-directed foods which effectively modulate the gut microbiome, may influence the regulation of obesity and associated comorbidities.
34579036	8	57	theme	novel	1674:1678	arg1	synbiotic					1691:1699	the novel ASEC-based synbiotic	1670:1699	the novel ASEC-based synbiotic	1670:1699	Collectively, our results suggest that delayed dietary intervention with the novel ASEC-based synbiotic ameliorates HFD-induced obesity, metabolic disorders, and dysbiosis.
34579036	5	58	theme	liver	1120:1124	arg1	tissues					1126:1132	the adipose and liver tissues	1104:1132	tissues	1126:1132	The ASEC-based synbiotic intervention helped improve HFD-induced body weight gain, hyperlipidemia, impaired glucose tolerance, insulin resistance, and inflammation of the adipose and liver tissues.
34579036	5	59	theme	glucose	1045:1051	arg1	tolerance					1053:1061	impaired glucose tolerance	1036:1061	impaired glucose tolerance	1036:1061	The ASEC-based synbiotic intervention helped improve HFD-induced body weight gain, hyperlipidemia, impaired glucose tolerance, insulin resistance, and inflammation of the adipose and liver tissues.
34579036	5	60	theme	impaired	1036:1043	arg1	tolerance					1053:1061	impaired glucose tolerance	1036:1061	impaired glucose tolerance	1036:1061	The ASEC-based synbiotic intervention helped improve HFD-induced body weight gain, hyperlipidemia, impaired glucose tolerance, insulin resistance, and inflammation of the adipose and liver tissues.
34579036	0	61	with	Intervention	10:21	arg1	Prebiotic					43:51	an Adlay-Based Prebiotic	28:51	an Adlay-Based Prebiotic	28:51	Synbiotic Intervention with an Adlay-Based Prebiotic and Probiotics Improved Diet-Induced Metabolic Disturbance in Mice by Modulation of the Gut Microbiota.
34579036	0	61	with	Intervention	10:21	arg1	Probiotics					57:66	Probiotics	57:66	Probiotics	57:66	Synbiotic Intervention with an Adlay-Based Prebiotic and Probiotics Improved Diet-Induced Metabolic Disturbance in Mice by Modulation of the Gut Microbiota.
34579036	2	62	theme	Dietary	313:319	arg1	interventions					321:333	Dietary interventions	313:333	Dietary interventions	313:333	Dietary interventions, including microbiota-directed foods which effectively modulate the gut microbiome, may influence the regulation of obesity and associated comorbidities.
34579036	2	62	theme	Dietary	313:319	arg1	foods					366:370	microbiota-directed foods	346:370	microbiota-directed foods which effectively modulate the gut microbiome	346:416	Dietary interventions, including microbiota-directed foods which effectively modulate the gut microbiome, may influence the regulation of obesity and associated comorbidities.
34579036	6	63	from	reconstitution	1239:1252	arg1	mice					1318:1321	HFD-induced obese mice	1300:1321	HFD-induced obese mice	1300:1321	In addition, data from fecal metagenomics indicated that the ASEC-based synbiotic intervention fostered reconstitution of gut bacterial diversity and composition in HFD-induced obese mice.
34579036	1	64	theme	modern	295:300	arg1	societies					302:310	modern societies	295:310	modern societies	295:310	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	7	65	theme	relative	1391:1398	arg1	abundance					1400:1408	the relative abundance	1387:1408	the relative abundance of families Ruminococcaceae and Muribaculaceae and order Bacteroidales	1387:1479	In particular, the ASEC-based synbiotic intervention increased the relative abundance of families Ruminococcaceae and Muribaculaceae and order Bacteroidales and reduced that of families Lactobacillaceae, Erysipelotrichaceae, and Streptococcaceae in HFD-induced obese mice.
34579036	5	66	theme	HFD-induced	990:1000	arg1	gain					1014:1017	HFD-induced body weight gain	990:1017	HFD-induced body weight gain	990:1017	The ASEC-based synbiotic intervention helped improve HFD-induced body weight gain, hyperlipidemia, impaired glucose tolerance, insulin resistance, and inflammation of the adipose and liver tissues.
34579036	4	67	theme	cooked	754:759	arg1	prebiotic					774:782	an adlay seed extrusion cooked (ASEC)-based prebiotic	730:782	an adlay seed extrusion cooked (ASEC)-based prebiotic	730:782	Here, we investigated the effects of a novel synbiotic intervention, consisting of an adlay seed extrusion cooked (ASEC)-based prebiotic and probiotic (Lactobacillus paracasei and Bacillus coagulans) on metabolic disorders and microbial dysbiosis in high-fat diet (HFD)-induced obese mice.
34579036	1	68	theme	type	227:230	arg1	T2DM					253:256	T2DM	253:256	T2DM	253:256	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	1	68	theme	type	227:230	arg1	mellitus					243:250	type 2 diabetes mellitus	227:250	type 2 diabetes mellitus (T2DM)	227:257	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	4	69	theme	diet	906:909	arg1	mice					931:934	high-fat diet (HFD)-induced obese mice	897:934	high-fat diet (HFD)-induced obese mice	897:934	Here, we investigated the effects of a novel synbiotic intervention, consisting of an adlay seed extrusion cooked (ASEC)-based prebiotic and probiotic (Lactobacillus paracasei and Bacillus coagulans) on metabolic disorders and microbial dysbiosis in high-fat diet (HFD)-induced obese mice.
34579036	7	70	theme	HFD-induced	1573:1583	arg1	mice					1591:1594	HFD-induced obese mice	1573:1594	HFD-induced obese mice	1573:1594	In particular, the ASEC-based synbiotic intervention increased the relative abundance of families Ruminococcaceae and Muribaculaceae and order Bacteroidales and reduced that of families Lactobacillaceae, Erysipelotrichaceae, and Streptococcaceae in HFD-induced obese mice.
34579036	5	71	theme	body	1002:1005	arg1	gain					1014:1017	HFD-induced body weight gain	990:1017	HFD-induced body weight gain	990:1017	The ASEC-based synbiotic intervention helped improve HFD-induced body weight gain, hyperlipidemia, impaired glucose tolerance, insulin resistance, and inflammation of the adipose and liver tissues.
34579036	1	72	theme	Metabolic	157:165	arg1	conditions					195:204	its associated conditions	180:204	its associated conditions	180:204	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	1	72	theme	Metabolic	157:165	arg1	issue					286:290	a major public health issue	264:290	a major public health issue in modern societies	264:310	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	1	72	theme	Metabolic	157:165	arg1	mellitus					243:250	type 2 diabetes mellitus	227:250	type 2 diabetes mellitus (T2DM)	227:257	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	1	72	theme	Metabolic	157:165	arg1	syndrome					167:174	Metabolic syndrome	157:174	Metabolic syndrome	157:174	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	1	72	theme	Metabolic	157:165	arg1	obesity					215:221	obesity	215:221	obesity	215:221	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	4	73	theme	microbial	874:882	arg1	dysbiosis					884:892	microbial dysbiosis	874:892	microbial dysbiosis	874:892	Here, we investigated the effects of a novel synbiotic intervention, consisting of an adlay seed extrusion cooked (ASEC)-based prebiotic and probiotic (Lactobacillus paracasei and Bacillus coagulans) on metabolic disorders and microbial dysbiosis in high-fat diet (HFD)-induced obese mice.
34579036	8	74	theme	ASEC-based	1680:1689	arg1	synbiotic					1691:1699	the novel ASEC-based synbiotic	1670:1699	the novel ASEC-based synbiotic	1670:1699	Collectively, our results suggest that delayed dietary intervention with the novel ASEC-based synbiotic ameliorates HFD-induced obesity, metabolic disorders, and dysbiosis.
34579036	0	75	theme	Diet-Induced	77:88	arg1	Disturbance					100:110	Diet-Induced Metabolic Disturbance	77:110	Diet-Induced Metabolic Disturbance	77:110	Synbiotic Intervention with an Adlay-Based Prebiotic and Probiotics Improved Diet-Induced Metabolic Disturbance in Mice by Modulation of the Gut Microbiota.
34579036	5	76	theme	insulin	1064:1070	arg1	resistance					1072:1081	insulin resistance	1064:1081	insulin resistance	1064:1081	The ASEC-based synbiotic intervention helped improve HFD-induced body weight gain, hyperlipidemia, impaired glucose tolerance, insulin resistance, and inflammation of the adipose and liver tissues.
34579036	3	77	theme	synbiotics	606:615	arg1	use					599:601	the use	595:601	the use of synbiotics	595:615	Although research on probiotics and prebiotics has been conducted extensively in recent years, diets with the use of synbiotics remain relatively unexplored.
34579036	4	78	theme	novel	686:690	arg1	intervention					702:713	a novel synbiotic intervention	684:713	a novel synbiotic intervention	684:713	Here, we investigated the effects of a novel synbiotic intervention, consisting of an adlay seed extrusion cooked (ASEC)-based prebiotic and probiotic (Lactobacillus paracasei and Bacillus coagulans) on metabolic disorders and microbial dysbiosis in high-fat diet (HFD)-induced obese mice.
34579036	4	79	dep	probiotic	788:796	arg1	paracasei					813:821	Lactobacillus paracasei	799:821	Lactobacillus paracasei	799:821	Here, we investigated the effects of a novel synbiotic intervention, consisting of an adlay seed extrusion cooked (ASEC)-based prebiotic and probiotic (Lactobacillus paracasei and Bacillus coagulans) on metabolic disorders and microbial dysbiosis in high-fat diet (HFD)-induced obese mice.
34579036	4	79	dep	probiotic	788:796	arg1	coagulans					836:844	Bacillus coagulans	827:844	Bacillus coagulans	827:844	Here, we investigated the effects of a novel synbiotic intervention, consisting of an adlay seed extrusion cooked (ASEC)-based prebiotic and probiotic (Lactobacillus paracasei and Bacillus coagulans) on metabolic disorders and microbial dysbiosis in high-fat diet (HFD)-induced obese mice.
34579036	1	80	theme	diabetes	234:241	arg1	T2DM					253:256	T2DM	253:256	T2DM	253:256	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	1	80	theme	diabetes	234:241	arg1	mellitus					243:250	type 2 diabetes mellitus	227:250	type 2 diabetes mellitus (T2DM)	227:257	Metabolic syndrome and its associated conditions, such as obesity and type 2 diabetes mellitus (T2DM), are a major public health issue in modern societies.
34579036	4	81	from	dysbiosis	884:892	arg1	mice					931:934	high-fat diet (HFD)-induced obese mice	897:934	high-fat diet (HFD)-induced obese mice	897:934	Here, we investigated the effects of a novel synbiotic intervention, consisting of an adlay seed extrusion cooked (ASEC)-based prebiotic and probiotic (Lactobacillus paracasei and Bacillus coagulans) on metabolic disorders and microbial dysbiosis in high-fat diet (HFD)-induced obese mice.
34579036	8	82	with	intervention	1652:1663	arg1	synbiotic					1691:1699	the novel ASEC-based synbiotic	1670:1699	the novel ASEC-based synbiotic	1670:1699	Collectively, our results suggest that delayed dietary intervention with the novel ASEC-based synbiotic ameliorates HFD-induced obesity, metabolic disorders, and dysbiosis.
34579036	2	83	theme	microbiota-directed	346:364	arg1	foods					366:370	microbiota-directed foods	346:370	microbiota-directed foods which effectively modulate the gut microbiome	346:416	Dietary interventions, including microbiota-directed foods which effectively modulate the gut microbiome, may influence the regulation of obesity and associated comorbidities.
34579036	7	84	theme	obese	1585:1589	arg1	mice					1591:1594	HFD-induced obese mice	1573:1594	HFD-induced obese mice	1573:1594	In particular, the ASEC-based synbiotic intervention increased the relative abundance of families Ruminococcaceae and Muribaculaceae and order Bacteroidales and reduced that of families Lactobacillaceae, Erysipelotrichaceae, and Streptococcaceae in HFD-induced obese mice.
33256764	0	0	theme	wound	93:97	arg1	healing					99:105	experimental diabetic wound healing	71:105	experimental diabetic wound healing	71:105	Preparation of PLA/chitosan nanoscaffolds containing cod liver oil and experimental diabetic wound healing in male rats study.
33256764	7	1	dep	structure	1082:1090	arg1	The					1078:1080	The	1078:1080	The	1078:1080	The structure and functional groups of the final products were characterized by Fourier-transform infrared spectroscopy (FT-IR) and energy dispersive spectroscopy (EDAX) as elemental analysis.
33256764	4	2	theme	lactic	689:694	arg1	nanoscaffolds					710:722	poly lactic acid/chitosan nanoscaffolds	684:722	poly lactic acid/chitosan nanoscaffolds	684:722	In this study, electrospinning method was used to produce poly lactic acid/chitosan nanoscaffolds as a suitable bio-substitute.
33256764	4	2	theme	lactic	689:694	arg1	bio-substitute					738:751	a suitable bio-substitute	727:751	a suitable bio-substitute	727:751	In this study, electrospinning method was used to produce poly lactic acid/chitosan nanoscaffolds as a suitable bio-substitute.
33256764	0	3	theme	diabetic	84:91	arg1	healing					99:105	experimental diabetic wound healing	71:105	experimental diabetic wound healing	71:105	Preparation of PLA/chitosan nanoscaffolds containing cod liver oil and experimental diabetic wound healing in male rats study.
33256764	4	4	theme	poly	684:687	arg1	nanoscaffolds					710:722	poly lactic acid/chitosan nanoscaffolds	684:722	poly lactic acid/chitosan nanoscaffolds	684:722	In this study, electrospinning method was used to produce poly lactic acid/chitosan nanoscaffolds as a suitable bio-substitute.
33256764	4	4	theme	poly	684:687	arg1	bio-substitute					738:751	a suitable bio-substitute	727:751	a suitable bio-substitute	727:751	In this study, electrospinning method was used to produce poly lactic acid/chitosan nanoscaffolds as a suitable bio-substitute.
33256764	9	5	theme	poly	1479:1482	arg1	nanoscaffolds					1505:1517	poly lactic acid/chitosan nanoscaffolds	1479:1517	poly lactic acid/chitosan nanoscaffolds containing cod liver oil	1479:1542	According to the results, poly lactic acid/chitosan nanoscaffolds containing cod liver oil can be a suitable bio-product to be used in treating the diabetic foot ulcer syndrome.
33256764	9	5	theme	poly	1479:1482	arg1	bio-product					1562:1572	a suitable bio-product	1551:1572	a suitable bio-product to be used in treating the diabetic foot ulcer syndrome	1551:1628	According to the results, poly lactic acid/chitosan nanoscaffolds containing cod liver oil can be a suitable bio-product to be used in treating the diabetic foot ulcer syndrome.
33256764	9	6	theme	ulcer	1615:1619	arg1	syndrome					1621:1628	the diabetic foot ulcer syndrome	1597:1628	the diabetic foot ulcer syndrome	1597:1628	According to the results, poly lactic acid/chitosan nanoscaffolds containing cod liver oil can be a suitable bio-product to be used in treating the diabetic foot ulcer syndrome.
33256764	7	7	theme	final	1121:1125	arg1	products					1127:1134	the final products	1117:1134	the final products	1117:1134	The structure and functional groups of the final products were characterized by Fourier-transform infrared spectroscopy (FT-IR) and energy dispersive spectroscopy (EDAX) as elemental analysis.
33256764	3	8	theme	phospholipids	564:576	arg1	composition					578:588	the membrane phospholipids composition	551:588	the membrane phospholipids composition	551:588	The formulation of nanoscaffolds containing cod liver oil by altering the hemodynamic balance toward the vasodilators state, increasing wound blood supply, and altering plasma membrane properties, namely altering the membrane phospholipids composition, can be effective in wound healing.
33256764	0	9	from	oil	63:65	arg1	rats					115:118	male rats	110:118	male rats	110:118	Preparation of PLA/chitosan nanoscaffolds containing cod liver oil and experimental diabetic wound healing in male rats study.
33256764	7	10	theme	products	1127:1134	arg1	products					1127:1134	the final products	1117:1134	the final products	1117:1134	The structure and functional groups of the final products were characterized by Fourier-transform infrared spectroscopy (FT-IR) and energy dispersive spectroscopy (EDAX) as elemental analysis.
33256764	7	10	theme	products	1127:1134	arg1	analysis					1261:1268	elemental analysis	1251:1268	elemental analysis	1251:1268	The structure and functional groups of the final products were characterized by Fourier-transform infrared spectroscopy (FT-IR) and energy dispersive spectroscopy (EDAX) as elemental analysis.
33256764	7	10	theme	products	1127:1134	arg1	structure					1082:1090	structure	1082:1090	structure	1082:1090	The structure and functional groups of the final products were characterized by Fourier-transform infrared spectroscopy (FT-IR) and energy dispersive spectroscopy (EDAX) as elemental analysis.
33256764	7	10	theme	products	1127:1134	arg1	groups					1107:1112	functional groups	1096:1112	functional groups	1096:1112	The structure and functional groups of the final products were characterized by Fourier-transform infrared spectroscopy (FT-IR) and energy dispersive spectroscopy (EDAX) as elemental analysis.
33256764	0	11	contain	containing	42:51	arg2	oil					63:65	cod liver oil	53:65	cod liver oil	53:65	Preparation of PLA/chitosan nanoscaffolds containing cod liver oil and experimental diabetic wound healing in male rats study.
33256764	0	11	contain	containing	42:51	arg1	nanoscaffolds					28:40	PLA/chitosan nanoscaffolds	15:40	PLA/chitosan nanoscaffolds containing cod liver oil and experimental diabetic wound healing in male rats	15:118	Preparation of PLA/chitosan nanoscaffolds containing cod liver oil and experimental diabetic wound healing in male rats study.
33256764	0	11	contain	containing	42:51	arg2	healing					99:105	experimental diabetic wound healing	71:105	experimental diabetic wound healing	71:105	Preparation of PLA/chitosan nanoscaffolds containing cod liver oil and experimental diabetic wound healing in male rats study.
33256764	9	12	theme	liver	1534:1538	arg1	oil					1540:1542	cod liver oil	1530:1542	cod liver oil	1530:1542	According to the results, poly lactic acid/chitosan nanoscaffolds containing cod liver oil can be a suitable bio-product to be used in treating the diabetic foot ulcer syndrome.
33256764	8	13	theme	cod	1322:1324	arg1	oil					1332:1334	cod liver oil	1322:1334	cod liver oil	1322:1334	The results showed that the optimum formulation of cod liver oil was 30%, which formed a very thin fiber that rapidly absorbed to the wound and produced significant healing effects.
33256764	7	14	theme	energy	1210:1215	arg1	spectroscopy					1228:1239	energy dispersive spectroscopy	1210:1239	energy dispersive spectroscopy (EDAX)	1210:1246	The structure and functional groups of the final products were characterized by Fourier-transform infrared spectroscopy (FT-IR) and energy dispersive spectroscopy (EDAX) as elemental analysis.
33256764	7	14	theme	energy	1210:1215	arg1	EDAX					1242:1245	EDAX	1242:1245	EDAX	1242:1245	The structure and functional groups of the final products were characterized by Fourier-transform infrared spectroscopy (FT-IR) and energy dispersive spectroscopy (EDAX) as elemental analysis.
33256764	5	15	theme	electron	910:917	arg1	microscopy					919:928	scanning electron microscopy	901:928	scanning electron microscopy (SEM)	901:934	After preparing the nanoscaffolds, the products were characterized with dynamic light scattering (DLS), transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
33256764	5	15	theme	electron	910:917	arg1	SEM					931:933	SEM	931:933	SEM	931:933	After preparing the nanoscaffolds, the products were characterized with dynamic light scattering (DLS), transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
33256764	6	16	theme	UV-Vis	1062:1067	arg1	spectra					1069:1075	UV-Vis spectra	1062:1075	UV-Vis spectra	1062:1075	Also optical properties of polymer and comparison between adsorption between single polymer and polymer-drug calculated with UV-Vis spectra.
33256764	3	17	theme	hemodynamic	412:422	arg1	balance					424:430	the hemodynamic balance	408:430	the hemodynamic balance toward the vasodilators state	408:460	The formulation of nanoscaffolds containing cod liver oil by altering the hemodynamic balance toward the vasodilators state, increasing wound blood supply, and altering plasma membrane properties, namely altering the membrane phospholipids composition, can be effective in wound healing.
33256764	5	18	theme	transmission	858:869	arg1	TEM					892:894	TEM	892:894	TEM	892:894	After preparing the nanoscaffolds, the products were characterized with dynamic light scattering (DLS), transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
33256764	5	18	theme	transmission	858:869	arg1	microscopy					880:889	transmission electron microscopy	858:889	transmission electron microscopy (TEM)	858:895	After preparing the nanoscaffolds, the products were characterized with dynamic light scattering (DLS), transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
33256764	0	19	theme	male	110:113	arg1	rats					115:118	male rats	110:118	male rats	110:118	Preparation of PLA/chitosan nanoscaffolds containing cod liver oil and experimental diabetic wound healing in male rats study.
33256764	5	20	theme	scanning	901:908	arg1	microscopy					919:928	scanning electron microscopy	901:928	scanning electron microscopy (SEM)	901:934	After preparing the nanoscaffolds, the products were characterized with dynamic light scattering (DLS), transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
33256764	5	20	theme	scanning	901:908	arg1	SEM					931:933	SEM	931:933	SEM	931:933	After preparing the nanoscaffolds, the products were characterized with dynamic light scattering (DLS), transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
33256764	7	21	theme	infrared	1176:1183	arg1	FT-IR					1199:1203	FT-IR	1199:1203	FT-IR	1199:1203	The structure and functional groups of the final products were characterized by Fourier-transform infrared spectroscopy (FT-IR) and energy dispersive spectroscopy (EDAX) as elemental analysis.
33256764	7	21	theme	infrared	1176:1183	arg1	spectroscopy					1185:1196	Fourier-transform infrared spectroscopy	1158:1196	Fourier-transform infrared spectroscopy (FT-IR)	1158:1204	The structure and functional groups of the final products were characterized by Fourier-transform infrared spectroscopy (FT-IR) and energy dispersive spectroscopy (EDAX) as elemental analysis.
33256764	3	22	contain	containing	371:380	arg1	nanoscaffolds					357:369	nanoscaffolds	357:369	nanoscaffolds containing cod liver oil	357:394	The formulation of nanoscaffolds containing cod liver oil by altering the hemodynamic balance toward the vasodilators state, increasing wound blood supply, and altering plasma membrane properties, namely altering the membrane phospholipids composition, can be effective in wound healing.
33256764	3	22	contain	containing	371:380	arg2	oil					392:394	cod liver oil	382:394	cod liver oil	382:394	The formulation of nanoscaffolds containing cod liver oil by altering the hemodynamic balance toward the vasodilators state, increasing wound blood supply, and altering plasma membrane properties, namely altering the membrane phospholipids composition, can be effective in wound healing.
33256764	2	23	theme	ulcer	266:270	arg1	syndrome					272:279	diabetic foot ulcer syndrome	252:279	diabetic foot ulcer syndrome	252:279	One of the important metabolic complications in diabetes is diabetic foot ulcer syndrome, which causes delayed and abnormal healing of the wound.
33256764	3	24	from	healing	617:623	arg1	effective					598:606	effective	598:606	effective	598:606	The formulation of nanoscaffolds containing cod liver oil by altering the hemodynamic balance toward the vasodilators state, increasing wound blood supply, and altering plasma membrane properties, namely altering the membrane phospholipids composition, can be effective in wound healing.
33256764	3	24	from	healing	617:623	arg1	formulation					342:352	The formulation	338:352	The formulation of nanoscaffolds containing cod liver oil	338:394	The formulation of nanoscaffolds containing cod liver oil by altering the hemodynamic balance toward the vasodilators state, increasing wound blood supply, and altering plasma membrane properties, namely altering the membrane phospholipids composition, can be effective in wound healing.
33256764	1	25	theme	common	164:169	arg1	disorders					181:189	the most common metabolic disorders	155:189	the most common metabolic disorders	155:189	Diabetes mellitus is one of the most common metabolic disorders.
33256764	3	26	theme	cod	382:384	arg1	oil					392:394	cod liver oil	382:394	cod liver oil	382:394	The formulation of nanoscaffolds containing cod liver oil by altering the hemodynamic balance toward the vasodilators state, increasing wound blood supply, and altering plasma membrane properties, namely altering the membrane phospholipids composition, can be effective in wound healing.
33256764	3	27	theme	nanoscaffolds	357:369	arg1	effective					598:606	effective	598:606	effective	598:606	The formulation of nanoscaffolds containing cod liver oil by altering the hemodynamic balance toward the vasodilators state, increasing wound blood supply, and altering plasma membrane properties, namely altering the membrane phospholipids composition, can be effective in wound healing.
33256764	3	27	theme	nanoscaffolds	357:369	arg1	formulation					342:352	The formulation	338:352	The formulation of nanoscaffolds containing cod liver oil	338:394	The formulation of nanoscaffolds containing cod liver oil by altering the hemodynamic balance toward the vasodilators state, increasing wound blood supply, and altering plasma membrane properties, namely altering the membrane phospholipids composition, can be effective in wound healing.
33256764	7	28	theme	functional	1096:1105	arg1	products					1127:1134	the final products	1117:1134	the final products	1117:1134	The structure and functional groups of the final products were characterized by Fourier-transform infrared spectroscopy (FT-IR) and energy dispersive spectroscopy (EDAX) as elemental analysis.
33256764	7	28	theme	functional	1096:1105	arg1	analysis					1261:1268	elemental analysis	1251:1268	elemental analysis	1251:1268	The structure and functional groups of the final products were characterized by Fourier-transform infrared spectroscopy (FT-IR) and energy dispersive spectroscopy (EDAX) as elemental analysis.
33256764	7	28	theme	functional	1096:1105	arg1	structure					1082:1090	structure	1082:1090	structure	1082:1090	The structure and functional groups of the final products were characterized by Fourier-transform infrared spectroscopy (FT-IR) and energy dispersive spectroscopy (EDAX) as elemental analysis.
33256764	7	28	theme	functional	1096:1105	arg1	groups					1107:1112	functional groups	1096:1112	functional groups	1096:1112	The structure and functional groups of the final products were characterized by Fourier-transform infrared spectroscopy (FT-IR) and energy dispersive spectroscopy (EDAX) as elemental analysis.
33256764	9	29	theme	suitable	1553:1560	arg1	nanoscaffolds					1505:1517	poly lactic acid/chitosan nanoscaffolds	1479:1517	poly lactic acid/chitosan nanoscaffolds containing cod liver oil	1479:1542	According to the results, poly lactic acid/chitosan nanoscaffolds containing cod liver oil can be a suitable bio-product to be used in treating the diabetic foot ulcer syndrome.
33256764	9	29	theme	suitable	1553:1560	arg1	bio-product					1562:1572	a suitable bio-product	1551:1572	a suitable bio-product to be used in treating the diabetic foot ulcer syndrome	1551:1628	According to the results, poly lactic acid/chitosan nanoscaffolds containing cod liver oil can be a suitable bio-product to be used in treating the diabetic foot ulcer syndrome.
33256764	2	30	theme	abnormal	307:314	arg1	healing					316:322	delayed and abnormal healing	295:322	delayed and abnormal healing of the wound	295:335	One of the important metabolic complications in diabetes is diabetic foot ulcer syndrome, which causes delayed and abnormal healing of the wound.
33256764	2	31	from	complications	223:235	arg1	diabetes					240:247	diabetes	240:247	diabetes	240:247	One of the important metabolic complications in diabetes is diabetic foot ulcer syndrome, which causes delayed and abnormal healing of the wound.
33256764	1	32	theme	metabolic	171:179	arg1	disorders					181:189	the most common metabolic disorders	155:189	the most common metabolic disorders	155:189	Diabetes mellitus is one of the most common metabolic disorders.
33256764	3	33	theme	liver	386:390	arg1	oil					392:394	cod liver oil	382:394	cod liver oil	382:394	The formulation of nanoscaffolds containing cod liver oil by altering the hemodynamic balance toward the vasodilators state, increasing wound blood supply, and altering plasma membrane properties, namely altering the membrane phospholipids composition, can be effective in wound healing.
33256764	4	34	theme	suitable	729:736	arg1	nanoscaffolds					710:722	poly lactic acid/chitosan nanoscaffolds	684:722	poly lactic acid/chitosan nanoscaffolds	684:722	In this study, electrospinning method was used to produce poly lactic acid/chitosan nanoscaffolds as a suitable bio-substitute.
33256764	4	34	theme	suitable	729:736	arg1	bio-substitute					738:751	a suitable bio-substitute	727:751	a suitable bio-substitute	727:751	In this study, electrospinning method was used to produce poly lactic acid/chitosan nanoscaffolds as a suitable bio-substitute.
33256764	1	35	theme	disorders	181:189	arg1	one					148:150	one	148:150	one	148:150	Diabetes mellitus is one of the most common metabolic disorders.
33256764	1	35	theme	disorders	181:189	arg1	disorders					181:189	the most common metabolic disorders	155:189	the most common metabolic disorders	155:189	Diabetes mellitus is one of the most common metabolic disorders.
33256764	0	36	theme	nanoscaffolds	28:40	arg1	Preparation					0:10	Preparation	0:10	Preparation of PLA/chitosan nanoscaffolds containing cod liver oil and experimental diabetic wound healing in male rats	0:118	Preparation of PLA/chitosan nanoscaffolds containing cod liver oil and experimental diabetic wound healing in male rats study.
33256764	5	37	theme	dynamic	826:832	arg1	DLS					852:854	DLS	852:854	DLS	852:854	After preparing the nanoscaffolds, the products were characterized with dynamic light scattering (DLS), transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
33256764	5	37	theme	dynamic	826:832	arg1	scattering					840:849	dynamic light scattering	826:849	dynamic light scattering (DLS)	826:855	After preparing the nanoscaffolds, the products were characterized with dynamic light scattering (DLS), transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
33256764	9	38	theme	diabetic	1601:1608	arg1	syndrome					1621:1628	the diabetic foot ulcer syndrome	1597:1628	the diabetic foot ulcer syndrome	1597:1628	According to the results, poly lactic acid/chitosan nanoscaffolds containing cod liver oil can be a suitable bio-product to be used in treating the diabetic foot ulcer syndrome.
33256764	2	39	theme	delayed	295:301	arg1	healing					316:322	delayed and abnormal healing	295:322	delayed and abnormal healing of the wound	295:335	One of the important metabolic complications in diabetes is diabetic foot ulcer syndrome, which causes delayed and abnormal healing of the wound.
33256764	0	40	theme	PLA/chitosan	15:26	arg1	nanoscaffolds					28:40	PLA/chitosan nanoscaffolds	15:40	PLA/chitosan nanoscaffolds containing cod liver oil and experimental diabetic wound healing in male rats	15:118	Preparation of PLA/chitosan nanoscaffolds containing cod liver oil and experimental diabetic wound healing in male rats study.
33256764	9	41	theme	acid/chitosan	1491:1503	arg1	nanoscaffolds					1505:1517	poly lactic acid/chitosan nanoscaffolds	1479:1517	poly lactic acid/chitosan nanoscaffolds containing cod liver oil	1479:1542	According to the results, poly lactic acid/chitosan nanoscaffolds containing cod liver oil can be a suitable bio-product to be used in treating the diabetic foot ulcer syndrome.
33256764	9	41	theme	acid/chitosan	1491:1503	arg1	bio-product					1562:1572	a suitable bio-product	1551:1572	a suitable bio-product to be used in treating the diabetic foot ulcer syndrome	1551:1628	According to the results, poly lactic acid/chitosan nanoscaffolds containing cod liver oil can be a suitable bio-product to be used in treating the diabetic foot ulcer syndrome.
33256764	3	42	theme	membrane	555:562	arg1	composition					578:588	the membrane phospholipids composition	551:588	the membrane phospholipids composition	551:588	The formulation of nanoscaffolds containing cod liver oil by altering the hemodynamic balance toward the vasodilators state, increasing wound blood supply, and altering plasma membrane properties, namely altering the membrane phospholipids composition, can be effective in wound healing.
33256764	5	43	theme	electron	871:878	arg1	TEM					892:894	TEM	892:894	TEM	892:894	After preparing the nanoscaffolds, the products were characterized with dynamic light scattering (DLS), transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
33256764	5	43	theme	electron	871:878	arg1	microscopy					880:889	transmission electron microscopy	858:889	transmission electron microscopy (TEM)	858:895	After preparing the nanoscaffolds, the products were characterized with dynamic light scattering (DLS), transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
33256764	0	44	theme	cod	53:55	arg1	oil					63:65	cod liver oil	53:65	cod liver oil	53:65	Preparation of PLA/chitosan nanoscaffolds containing cod liver oil and experimental diabetic wound healing in male rats study.
33256764	9	45	theme	lactic	1484:1489	arg1	nanoscaffolds					1505:1517	poly lactic acid/chitosan nanoscaffolds	1479:1517	poly lactic acid/chitosan nanoscaffolds containing cod liver oil	1479:1542	According to the results, poly lactic acid/chitosan nanoscaffolds containing cod liver oil can be a suitable bio-product to be used in treating the diabetic foot ulcer syndrome.
33256764	9	45	theme	lactic	1484:1489	arg1	bio-product					1562:1572	a suitable bio-product	1551:1572	a suitable bio-product to be used in treating the diabetic foot ulcer syndrome	1551:1628	According to the results, poly lactic acid/chitosan nanoscaffolds containing cod liver oil can be a suitable bio-product to be used in treating the diabetic foot ulcer syndrome.
33256764	8	46	theme	healing	1436:1442	arg1	effects					1444:1450	significant healing effects	1424:1450	significant healing effects	1424:1450	The results showed that the optimum formulation of cod liver oil was 30%, which formed a very thin fiber that rapidly absorbed to the wound and produced significant healing effects.
33256764	3	47	from	effective	598:606	arg1	healing					617:623	wound healing	611:623	wound healing	611:623	The formulation of nanoscaffolds containing cod liver oil by altering the hemodynamic balance toward the vasodilators state, increasing wound blood supply, and altering plasma membrane properties, namely altering the membrane phospholipids composition, can be effective in wound healing.
33256764	3	48	theme	vasodilators	443:454	arg1	state					456:460	the vasodilators state	439:460	the vasodilators state	439:460	The formulation of nanoscaffolds containing cod liver oil by altering the hemodynamic balance toward the vasodilators state, increasing wound blood supply, and altering plasma membrane properties, namely altering the membrane phospholipids composition, can be effective in wound healing.
33256764	6	49	theme	optical	942:948	arg1	properties					950:959	optical properties	942:959	optical properties of polymer	942:970	Also optical properties of polymer and comparison between adsorption between single polymer and polymer-drug calculated with UV-Vis spectra.
33256764	8	50	theme	liver	1326:1330	arg1	oil					1332:1334	cod liver oil	1322:1334	cod liver oil	1322:1334	The results showed that the optimum formulation of cod liver oil was 30%, which formed a very thin fiber that rapidly absorbed to the wound and produced significant healing effects.
33256764	8	51	theme	oil	1332:1334	arg1	formulation					1307:1317	the optimum formulation	1295:1317	the optimum formulation of cod liver oil	1295:1334	The results showed that the optimum formulation of cod liver oil was 30%, which formed a very thin fiber that rapidly absorbed to the wound and produced significant healing effects.
33256764	8	51	theme	oil	1332:1334	arg1	%					1342:1342	30%	1340:1342	30%	1340:1342	The results showed that the optimum formulation of cod liver oil was 30%, which formed a very thin fiber that rapidly absorbed to the wound and produced significant healing effects.
33256764	2	52	theme	complications	223:235	arg1	complications					223:235	the important metabolic complications	199:235	the important metabolic complications in diabetes	199:247	One of the important metabolic complications in diabetes is diabetic foot ulcer syndrome, which causes delayed and abnormal healing of the wound.
33256764	2	52	theme	complications	223:235	arg1	One					192:194	One	192:194	One	192:194	One of the important metabolic complications in diabetes is diabetic foot ulcer syndrome, which causes delayed and abnormal healing of the wound.
33256764	5	53	theme	light	834:838	arg1	DLS					852:854	DLS	852:854	DLS	852:854	After preparing the nanoscaffolds, the products were characterized with dynamic light scattering (DLS), transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
33256764	5	53	theme	light	834:838	arg1	scattering					840:849	dynamic light scattering	826:849	dynamic light scattering (DLS)	826:855	After preparing the nanoscaffolds, the products were characterized with dynamic light scattering (DLS), transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
33256764	8	54	theme	thin	1365:1368	arg1	fiber					1370:1374	a very thin fiber	1358:1374	a very thin fiber that rapidly absorbed to the wound and produced significant healing effects	1358:1450	The results showed that the optimum formulation of cod liver oil was 30%, which formed a very thin fiber that rapidly absorbed to the wound and produced significant healing effects.
33256764	6	55	theme	polymer	964:970	arg1	comparison					976:985	comparison	976:985	comparison between adsorption between single polymer and polymer-drug	976:1044	Also optical properties of polymer and comparison between adsorption between single polymer and polymer-drug calculated with UV-Vis spectra.
33256764	6	55	theme	polymer	964:970	arg1	properties					950:959	optical properties	942:959	optical properties of polymer	942:970	Also optical properties of polymer and comparison between adsorption between single polymer and polymer-drug calculated with UV-Vis spectra.
33256764	2	56	theme	metabolic	213:221	arg1	complications					223:235	the important metabolic complications	199:235	the important metabolic complications in diabetes	199:247	One of the important metabolic complications in diabetes is diabetic foot ulcer syndrome, which causes delayed and abnormal healing of the wound.
33256764	4	57	used	used	668:671	arg2	method					657:662	electrospinning method	641:662	electrospinning method	641:662	In this study, electrospinning method was used to produce poly lactic acid/chitosan nanoscaffolds as a suitable bio-substitute.
33256764	2	58	theme	wound	331:335	arg1	healing					316:322	delayed and abnormal healing	295:322	delayed and abnormal healing of the wound	295:335	One of the important metabolic complications in diabetes is diabetic foot ulcer syndrome, which causes delayed and abnormal healing of the wound.
33256764	0	59	theme	liver	57:61	arg1	oil					63:65	cod liver oil	53:65	cod liver oil	53:65	Preparation of PLA/chitosan nanoscaffolds containing cod liver oil and experimental diabetic wound healing in male rats study.
33256764	7	60	theme	dispersive	1217:1226	arg1	spectroscopy					1228:1239	energy dispersive spectroscopy	1210:1239	energy dispersive spectroscopy (EDAX)	1210:1246	The structure and functional groups of the final products were characterized by Fourier-transform infrared spectroscopy (FT-IR) and energy dispersive spectroscopy (EDAX) as elemental analysis.
33256764	7	60	theme	dispersive	1217:1226	arg1	EDAX					1242:1245	EDAX	1242:1245	EDAX	1242:1245	The structure and functional groups of the final products were characterized by Fourier-transform infrared spectroscopy (FT-IR) and energy dispersive spectroscopy (EDAX) as elemental analysis.
33256764	2	61	theme	important	203:211	arg1	complications					223:235	the important metabolic complications	199:235	the important metabolic complications in diabetes	199:247	One of the important metabolic complications in diabetes is diabetic foot ulcer syndrome, which causes delayed and abnormal healing of the wound.
33256764	4	62	theme	acid/chitosan	696:708	arg1	nanoscaffolds					710:722	poly lactic acid/chitosan nanoscaffolds	684:722	poly lactic acid/chitosan nanoscaffolds	684:722	In this study, electrospinning method was used to produce poly lactic acid/chitosan nanoscaffolds as a suitable bio-substitute.
33256764	4	62	theme	acid/chitosan	696:708	arg1	bio-substitute					738:751	a suitable bio-substitute	727:751	a suitable bio-substitute	727:751	In this study, electrospinning method was used to produce poly lactic acid/chitosan nanoscaffolds as a suitable bio-substitute.
33256764	7	63	theme	Fourier-transform	1158:1174	arg1	FT-IR					1199:1203	FT-IR	1199:1203	FT-IR	1199:1203	The structure and functional groups of the final products were characterized by Fourier-transform infrared spectroscopy (FT-IR) and energy dispersive spectroscopy (EDAX) as elemental analysis.
33256764	7	63	theme	Fourier-transform	1158:1174	arg1	spectroscopy					1185:1196	Fourier-transform infrared spectroscopy	1158:1196	Fourier-transform infrared spectroscopy (FT-IR)	1158:1204	The structure and functional groups of the final products were characterized by Fourier-transform infrared spectroscopy (FT-IR) and energy dispersive spectroscopy (EDAX) as elemental analysis.
33256764	9	64	theme	foot	1610:1613	arg1	syndrome					1621:1628	the diabetic foot ulcer syndrome	1597:1628	the diabetic foot ulcer syndrome	1597:1628	According to the results, poly lactic acid/chitosan nanoscaffolds containing cod liver oil can be a suitable bio-product to be used in treating the diabetic foot ulcer syndrome.
33256764	2	65	theme	foot	261:264	arg1	syndrome					272:279	diabetic foot ulcer syndrome	252:279	diabetic foot ulcer syndrome	252:279	One of the important metabolic complications in diabetes is diabetic foot ulcer syndrome, which causes delayed and abnormal healing of the wound.
33256764	9	66	theme	cod	1530:1532	arg1	oil					1540:1542	cod liver oil	1530:1542	cod liver oil	1530:1542	According to the results, poly lactic acid/chitosan nanoscaffolds containing cod liver oil can be a suitable bio-product to be used in treating the diabetic foot ulcer syndrome.
33256764	1	67	theme	Diabetes	127:134	arg1	mellitus					136:143	Diabetes mellitus	127:143	Diabetes mellitus	127:143	Diabetes mellitus is one of the most common metabolic disorders.
33256764	9	68	contain	containing	1519:1528	arg2	oil					1540:1542	cod liver oil	1530:1542	cod liver oil	1530:1542	According to the results, poly lactic acid/chitosan nanoscaffolds containing cod liver oil can be a suitable bio-product to be used in treating the diabetic foot ulcer syndrome.
33256764	9	68	contain	containing	1519:1528	arg1	nanoscaffolds					1505:1517	poly lactic acid/chitosan nanoscaffolds	1479:1517	poly lactic acid/chitosan nanoscaffolds containing cod liver oil	1479:1542	According to the results, poly lactic acid/chitosan nanoscaffolds containing cod liver oil can be a suitable bio-product to be used in treating the diabetic foot ulcer syndrome.
33256764	9	68	contain	containing	1519:1528	arg1	bio-product					1562:1572	a suitable bio-product	1551:1572	a suitable bio-product to be used in treating the diabetic foot ulcer syndrome	1551:1628	According to the results, poly lactic acid/chitosan nanoscaffolds containing cod liver oil can be a suitable bio-product to be used in treating the diabetic foot ulcer syndrome.
33256764	8	69	theme	optimum	1299:1305	arg1	formulation					1307:1317	the optimum formulation	1295:1317	the optimum formulation of cod liver oil	1295:1334	The results showed that the optimum formulation of cod liver oil was 30%, which formed a very thin fiber that rapidly absorbed to the wound and produced significant healing effects.
33256764	8	69	theme	optimum	1299:1305	arg1	%					1342:1342	30%	1340:1342	30%	1340:1342	The results showed that the optimum formulation of cod liver oil was 30%, which formed a very thin fiber that rapidly absorbed to the wound and produced significant healing effects.
33256764	2	70	theme	diabetic	252:259	arg1	syndrome					272:279	diabetic foot ulcer syndrome	252:279	diabetic foot ulcer syndrome	252:279	One of the important metabolic complications in diabetes is diabetic foot ulcer syndrome, which causes delayed and abnormal healing of the wound.
33256764	3	71	theme	blood	480:484	arg1	supply					486:491	wound blood supply	474:491	wound blood supply	474:491	The formulation of nanoscaffolds containing cod liver oil by altering the hemodynamic balance toward the vasodilators state, increasing wound blood supply, and altering plasma membrane properties, namely altering the membrane phospholipids composition, can be effective in wound healing.
33256764	3	72	theme	wound	474:478	arg1	supply					486:491	wound blood supply	474:491	wound blood supply	474:491	The formulation of nanoscaffolds containing cod liver oil by altering the hemodynamic balance toward the vasodilators state, increasing wound blood supply, and altering plasma membrane properties, namely altering the membrane phospholipids composition, can be effective in wound healing.
33256764	0	73	theme	experimental	71:82	arg1	healing					99:105	experimental diabetic wound healing	71:105	experimental diabetic wound healing	71:105	Preparation of PLA/chitosan nanoscaffolds containing cod liver oil and experimental diabetic wound healing in male rats study.
33256764	0	74	from	healing	99:105	arg1	rats					115:118	male rats	110:118	male rats	110:118	Preparation of PLA/chitosan nanoscaffolds containing cod liver oil and experimental diabetic wound healing in male rats study.
33256764	8	75	theme	significant	1424:1434	arg1	effects					1444:1450	significant healing effects	1424:1450	significant healing effects	1424:1450	The results showed that the optimum formulation of cod liver oil was 30%, which formed a very thin fiber that rapidly absorbed to the wound and produced significant healing effects.
33256764	3	76	theme	plasma	507:512	arg1	properties					523:532	plasma membrane properties	507:532	plasma membrane properties	507:532	The formulation of nanoscaffolds containing cod liver oil by altering the hemodynamic balance toward the vasodilators state, increasing wound blood supply, and altering plasma membrane properties, namely altering the membrane phospholipids composition, can be effective in wound healing.
33256764	4	77	theme	electrospinning	641:655	arg1	method					657:662	electrospinning method	641:662	electrospinning method	641:662	In this study, electrospinning method was used to produce poly lactic acid/chitosan nanoscaffolds as a suitable bio-substitute.
33256764	7	78	theme	elemental	1251:1259	arg1	products					1127:1134	the final products	1117:1134	the final products	1117:1134	The structure and functional groups of the final products were characterized by Fourier-transform infrared spectroscopy (FT-IR) and energy dispersive spectroscopy (EDAX) as elemental analysis.
33256764	7	78	theme	elemental	1251:1259	arg1	analysis					1261:1268	elemental analysis	1251:1268	elemental analysis	1251:1268	The structure and functional groups of the final products were characterized by Fourier-transform infrared spectroscopy (FT-IR) and energy dispersive spectroscopy (EDAX) as elemental analysis.
33256764	7	78	theme	elemental	1251:1259	arg1	structure					1082:1090	structure	1082:1090	structure	1082:1090	The structure and functional groups of the final products were characterized by Fourier-transform infrared spectroscopy (FT-IR) and energy dispersive spectroscopy (EDAX) as elemental analysis.
33256764	7	78	theme	elemental	1251:1259	arg1	groups					1107:1112	functional groups	1096:1112	functional groups	1096:1112	The structure and functional groups of the final products were characterized by Fourier-transform infrared spectroscopy (FT-IR) and energy dispersive spectroscopy (EDAX) as elemental analysis.
33256764	3	79	theme	wound	611:615	arg1	healing					617:623	wound healing	611:623	wound healing	611:623	The formulation of nanoscaffolds containing cod liver oil by altering the hemodynamic balance toward the vasodilators state, increasing wound blood supply, and altering plasma membrane properties, namely altering the membrane phospholipids composition, can be effective in wound healing.
33256764	3	80	theme	membrane	514:521	arg1	properties					523:532	plasma membrane properties	507:532	plasma membrane properties	507:532	The formulation of nanoscaffolds containing cod liver oil by altering the hemodynamic balance toward the vasodilators state, increasing wound blood supply, and altering plasma membrane properties, namely altering the membrane phospholipids composition, can be effective in wound healing.
33256764	6	81	theme	single	1014:1019	arg1	polymer					1021:1027	single polymer	1014:1027	single polymer	1014:1027	Also optical properties of polymer and comparison between adsorption between single polymer and polymer-drug calculated with UV-Vis spectra.
34369950	7	0	theme	lipid	1343:1347	arg1	levels					1349:1354	the serum lipid levels	1333:1354	the serum lipid levels	1333:1354	In vivo, IDF supplementation reduced the serum lipid levels and inhibited hepatic fat accumulation.
34369950	4	1	theme	SIDF	809:812	arg1	%					820:820	90.50%	815:820	90.50%	815:820	The results showed that the main monosaccharides of SIDF (90.50%) identified were galactose, arabinose, xylose, rhamnose and glucose.
34369950	4	1	theme	SIDF	809:812	arg1	monosaccharides					790:804	the main monosaccharides	781:804	the main monosaccharides of SIDF (90.50%) identified	781:832	The results showed that the main monosaccharides of SIDF (90.50%) identified were galactose, arabinose, xylose, rhamnose and glucose.
34369950	4	1	theme	SIDF	809:812	arg1	galactose					839:847	galactose	839:847	galactose	839:847	The results showed that the main monosaccharides of SIDF (90.50%) identified were galactose, arabinose, xylose, rhamnose and glucose.
34369950	1	2	theme	lipid	239:243	arg1	metabolism					245:254	lipid metabolism	239:254	lipid metabolism	239:254	The potential benefits of insoluble dietary fiber (IDF) in the regulation of lipid metabolism have been reported in large prospective cohort studies although the molecular regulatory mechanism is still unclear.
34369950	10	3	theme	positive	1792:1799	arg1	SIDF					1702:1705	SIDF	1702:1705	SIDF	1702:1705	The above results show that SIDF could be used as an ideal functional ingredient in food processing as well as play a positive role in improving the added value of okara and promoting its comprehensive utilization.
34369950	10	3	theme	positive	1792:1799	arg1	role					1801:1804	a positive role	1790:1804	a positive role	1790:1804	The above results show that SIDF could be used as an ideal functional ingredient in food processing as well as play a positive role in improving the added value of okara and promoting its comprehensive utilization.
34369950	10	3	theme	positive	1792:1799	arg1	ingredient					1744:1753	an ideal functional ingredient	1724:1753	an ideal functional ingredient in food processing	1724:1772	The above results show that SIDF could be used as an ideal functional ingredient in food processing as well as play a positive role in improving the added value of okara and promoting its comprehensive utilization.
34369950	5	4	dep	transform	935:943	arg1	infrared					945:952	infrared	945:952	transform infrared spectroscopy (FT-IR)	935:973	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses suggested that SIDF had a loose and porous structure, polysaccharide functional groups, and a typical crystalline cellulose I structure.
34369950	6	5	dep	capacity	1193:1200	arg1	OAC					1203:1205	OAC	1203:1205	OAC; 7.95 g g-1	1203:1217	In addition, SIDF had ideal oil-adsorption capacity (OAC; 7.95 g g-1) and significantly improved cholesterol adsorption (11.14 mg g-1) at pH 7.0.
34369950	9	6	theme	systematic	1546:1555	arg1	This					1528:1531	This	1528:1531	This	1528:1531	This is the first systematic study on the composition, structure, physicochemical properties, adsorption function and biological effects of SIDF.
34369950	9	6	theme	systematic	1546:1555	arg1	study					1557:1561	the first systematic study	1536:1561	the first systematic study on the composition, structure, physicochemical properties, adsorption function and biological effects of SIDF	1536:1671	This is the first systematic study on the composition, structure, physicochemical properties, adsorption function and biological effects of SIDF.
34369950	1	7	theme	insoluble	188:196	arg1	fiber					206:210	insoluble dietary fiber	188:210	insoluble dietary fiber (IDF)	188:216	The potential benefits of insoluble dietary fiber (IDF) in the regulation of lipid metabolism have been reported in large prospective cohort studies although the molecular regulatory mechanism is still unclear.
34369950	1	7	theme	insoluble	188:196	arg1	IDF					213:215	IDF	213:215	IDF	213:215	The potential benefits of insoluble dietary fiber (IDF) in the regulation of lipid metabolism have been reported in large prospective cohort studies although the molecular regulatory mechanism is still unclear.
34369950	6	8	dep	OAC	1203:1205	arg1	g-1					1215:1217	7.95 g g-1	1208:1217	OAC; 7.95 g g-1	1203:1217	In addition, SIDF had ideal oil-adsorption capacity (OAC; 7.95 g g-1) and significantly improved cholesterol adsorption (11.14 mg g-1) at pH 7.0.
34369950	9	9	from	study	1557:1561	arg1	function					1633:1640	adsorption function	1622:1640	adsorption function	1622:1640	This is the first systematic study on the composition, structure, physicochemical properties, adsorption function and biological effects of SIDF.
34369950	9	9	from	study	1557:1561	arg1	effects					1657:1663	biological effects	1646:1663	biological effects	1646:1663	This is the first systematic study on the composition, structure, physicochemical properties, adsorption function and biological effects of SIDF.
34369950	9	9	from	study	1557:1561	arg1	structure					1583:1591	structure	1583:1591	structure	1583:1591	This is the first systematic study on the composition, structure, physicochemical properties, adsorption function and biological effects of SIDF.
34369950	9	9	from	study	1557:1561	arg1	composition					1570:1580	composition	1570:1580	composition	1570:1580	This is the first systematic study on the composition, structure, physicochemical properties, adsorption function and biological effects of SIDF.
34369950	9	9	from	study	1557:1561	arg1	properties					1610:1619	physicochemical properties	1594:1619	physicochemical properties	1594:1619	This is the first systematic study on the composition, structure, physicochemical properties, adsorption function and biological effects of SIDF.
34369950	2	10	theme	potential	512:520	arg1	fiber					530:534	potential dietary fiber	512:534	potential dietary fiber (DF)	512:539	Okara is a by-product obtained during soybean processing for soy milk and soybean curd (tofu), which is rarely utilized and can be a cheap potential dietary fiber (DF) resource.
34369950	2	10	theme	potential	512:520	arg1	DF					537:538	DF	537:538	DF	537:538	Okara is a by-product obtained during soybean processing for soy milk and soybean curd (tofu), which is rarely utilized and can be a cheap potential dietary fiber (DF) resource.
34369950	6	11	theme	g	1213:1213	arg1	g-1					1215:1217	7.95 g g-1	1208:1217	OAC; 7.95 g g-1	1203:1217	In addition, SIDF had ideal oil-adsorption capacity (OAC; 7.95 g g-1) and significantly improved cholesterol adsorption (11.14 mg g-1) at pH 7.0.
34369950	1	12	theme	fiber	206:210	arg1	benefits					176:183	The potential benefits	162:183	The potential benefits of insoluble dietary fiber (IDF) in the regulation of lipid metabolism	162:254	The potential benefits of insoluble dietary fiber (IDF) in the regulation of lipid metabolism have been reported in large prospective cohort studies although the molecular regulatory mechanism is still unclear.
34369950	10	13	theme	okara	1838:1842	arg1	value					1829:1833	the added value	1819:1833	the added value of okara	1819:1842	The above results show that SIDF could be used as an ideal functional ingredient in food processing as well as play a positive role in improving the added value of okara and promoting its comprehensive utilization.
34369950	3	14	theme	fat	715:717	arg1	metabolism					719:728	fat metabolism	715:728	fat metabolism	715:728	In this study, the structure and physicochemical properties of insoluble dietary fiber (SIDF) extracted from okara were characterized, and the prebiotic effects on fat metabolism were investigated in vivo.
34369950	0	15	theme	high	134:137	arg1	mice					156:159	high fat-fed C57BL/6J mice	134:159	high fat-fed C57BL/6J mice	134:159	Preparation and characterization of soybean insoluble dietary fiber and its prebiotic effect on dyslipidemia and hepatic steatosis in high fat-fed C57BL/6J mice.
34369950	5	16	theme	X-ray	980:984	arg1	XRD					999:1001	XRD	999:1001	XRD	999:1001	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses suggested that SIDF had a loose and porous structure, polysaccharide functional groups, and a typical crystalline cellulose I structure.
34369950	5	16	theme	X-ray	980:984	arg1	diffraction					986:996	X-ray diffraction	980:996	X-ray diffraction (XRD)	980:1002	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses suggested that SIDF had a loose and porous structure, polysaccharide functional groups, and a typical crystalline cellulose I structure.
34369950	10	17	theme	above	1678:1682	arg1	results					1684:1690	The above results	1674:1690	The above results	1674:1690	The above results show that SIDF could be used as an ideal functional ingredient in food processing as well as play a positive role in improving the added value of okara and promoting its comprehensive utilization.
34369950	0	18	theme	C57BL/6J	147:154	arg1	mice					156:159	high fat-fed C57BL/6J mice	134:159	high fat-fed C57BL/6J mice	134:159	Preparation and characterization of soybean insoluble dietary fiber and its prebiotic effect on dyslipidemia and hepatic steatosis in high fat-fed C57BL/6J mice.
34369950	9	19	theme	physicochemical	1594:1608	arg1	properties					1610:1619	physicochemical properties	1594:1619	physicochemical properties	1594:1619	This is the first systematic study on the composition, structure, physicochemical properties, adsorption function and biological effects of SIDF.
34369950	10	20	theme	comprehensive	1862:1874	arg1	utilization					1876:1886	its comprehensive utilization	1858:1886	its comprehensive utilization	1858:1886	The above results show that SIDF could be used as an ideal functional ingredient in food processing as well as play a positive role in improving the added value of okara and promoting its comprehensive utilization.
34369950	6	21	theme	oil-adsorption	1178:1191	arg1	capacity					1193:1200	ideal oil-adsorption capacity	1172:1200	ideal oil-adsorption capacity (OAC; 7.95 g g-1)	1172:1218	In addition, SIDF had ideal oil-adsorption capacity (OAC; 7.95 g g-1) and significantly improved cholesterol adsorption (11.14 mg g-1) at pH 7.0.
34369950	5	22	contain	had	1033:1035	arg2	groups					1093:1098	polysaccharide functional groups	1067:1098	polysaccharide functional groups	1067:1098	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses suggested that SIDF had a loose and porous structure, polysaccharide functional groups, and a typical crystalline cellulose I structure.
34369950	5	22	contain	had	1033:1035	arg1	SIDF					1028:1031	SIDF	1028:1031	SIDF	1028:1031	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses suggested that SIDF had a loose and porous structure, polysaccharide functional groups, and a typical crystalline cellulose I structure.
34369950	5	22	contain	had	1033:1035	arg2	structure					1056:1064	a loose and porous structure	1037:1064	structure	1056:1064	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses suggested that SIDF had a loose and porous structure, polysaccharide functional groups, and a typical crystalline cellulose I structure.
34369950	5	22	contain	had	1033:1035	arg2	structure					1139:1147	a typical crystalline cellulose I structure	1105:1147	a typical crystalline cellulose I structure	1105:1147	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses suggested that SIDF had a loose and porous structure, polysaccharide functional groups, and a typical crystalline cellulose I structure.
34369950	0	23	from	characterization	16:31	arg1	dyslipidemia					96:107	dyslipidemia	96:107	dyslipidemia	96:107	Preparation and characterization of soybean insoluble dietary fiber and its prebiotic effect on dyslipidemia and hepatic steatosis in high fat-fed C57BL/6J mice.
34369950	0	23	from	characterization	16:31	arg1	steatosis					121:129	hepatic steatosis	113:129	hepatic steatosis	113:129	Preparation and characterization of soybean insoluble dietary fiber and its prebiotic effect on dyslipidemia and hepatic steatosis in high fat-fed C57BL/6J mice.
34369950	3	24	from	effects	704:710	arg1	metabolism					719:728	fat metabolism	715:728	fat metabolism	715:728	In this study, the structure and physicochemical properties of insoluble dietary fiber (SIDF) extracted from okara were characterized, and the prebiotic effects on fat metabolism were investigated in vivo.
34369950	0	25	theme	prebiotic	76:84	arg1	effect					86:91	its prebiotic effect	72:91	its prebiotic effect on dyslipidemia and hepatic steatosis in high fat-fed C57BL/6J mice	72:159	Preparation and characterization of soybean insoluble dietary fiber and its prebiotic effect on dyslipidemia and hepatic steatosis in high fat-fed C57BL/6J mice.
34369950	1	26	theme	regulatory	334:343	arg1	mechanism					345:353	the molecular regulatory mechanism	320:353	the molecular regulatory mechanism	320:353	The potential benefits of insoluble dietary fiber (IDF) in the regulation of lipid metabolism have been reported in large prospective cohort studies although the molecular regulatory mechanism is still unclear.
34369950	1	26	theme	regulatory	334:343	arg1	unclear					364:370	unclear	364:370	unclear	364:370	The potential benefits of insoluble dietary fiber (IDF) in the regulation of lipid metabolism have been reported in large prospective cohort studies although the molecular regulatory mechanism is still unclear.
34369950	9	27	theme	biological	1646:1655	arg1	effects					1657:1663	biological effects	1646:1663	biological effects	1646:1663	This is the first systematic study on the composition, structure, physicochemical properties, adsorption function and biological effects of SIDF.
34369950	0	28	from	Preparation	0:10	arg1	dyslipidemia					96:107	dyslipidemia	96:107	dyslipidemia	96:107	Preparation and characterization of soybean insoluble dietary fiber and its prebiotic effect on dyslipidemia and hepatic steatosis in high fat-fed C57BL/6J mice.
34369950	0	28	from	Preparation	0:10	arg1	steatosis					121:129	hepatic steatosis	113:129	hepatic steatosis	113:129	Preparation and characterization of soybean insoluble dietary fiber and its prebiotic effect on dyslipidemia and hepatic steatosis in high fat-fed C57BL/6J mice.
34369950	5	29	theme	electron	900:907	arg1	SEM					921:923	SEM	921:923	SEM	921:923	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses suggested that SIDF had a loose and porous structure, polysaccharide functional groups, and a typical crystalline cellulose I structure.
34369950	5	29	theme	electron	900:907	arg1	microscopy					909:918	Scanning electron microscopy	891:918	Scanning electron microscopy (SEM)	891:924	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses suggested that SIDF had a loose and porous structure, polysaccharide functional groups, and a typical crystalline cellulose I structure.
34369950	6	30	contain	had	1168:1170	arg1	SIDF					1163:1166	SIDF	1163:1166	SIDF	1163:1166	In addition, SIDF had ideal oil-adsorption capacity (OAC; 7.95 g g-1) and significantly improved cholesterol adsorption (11.14 mg g-1) at pH 7.0.
34369950	6	30	contain	had	1168:1170	arg2	adsorption					1259:1268	significantly improved cholesterol adsorption	1224:1268	significantly improved cholesterol adsorption (11.14 mg g-1)	1224:1283	In addition, SIDF had ideal oil-adsorption capacity (OAC; 7.95 g g-1) and significantly improved cholesterol adsorption (11.14 mg g-1) at pH 7.0.
34369950	6	30	contain	had	1168:1170	arg2	g-1					1280:1282	11.14 mg g-1	1271:1282	11.14 mg g-1	1271:1282	In addition, SIDF had ideal oil-adsorption capacity (OAC; 7.95 g g-1) and significantly improved cholesterol adsorption (11.14 mg g-1) at pH 7.0.
34369950	6	30	contain	had	1168:1170	arg2	capacity					1193:1200	ideal oil-adsorption capacity	1172:1200	ideal oil-adsorption capacity (OAC; 7.95 g g-1)	1172:1218	In addition, SIDF had ideal oil-adsorption capacity (OAC; 7.95 g g-1) and significantly improved cholesterol adsorption (11.14 mg g-1) at pH 7.0.
34369950	5	31	theme	functional	1082:1091	arg1	groups					1093:1098	polysaccharide functional groups	1067:1098	polysaccharide functional groups	1067:1098	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses suggested that SIDF had a loose and porous structure, polysaccharide functional groups, and a typical crystalline cellulose I structure.
34369950	9	32	dep	composition	1570:1580	arg1	the					1566:1568	the	1566:1568	the	1566:1568	This is the first systematic study on the composition, structure, physicochemical properties, adsorption function and biological effects of SIDF.
34369950	2	33	theme	soy	434:436	arg1	milk					438:441	soy milk	434:441	soy milk	434:441	Okara is a by-product obtained during soybean processing for soy milk and soybean curd (tofu), which is rarely utilized and can be a cheap potential dietary fiber (DF) resource.
34369950	5	34	theme	polysaccharide	1067:1080	arg1	groups					1093:1098	polysaccharide functional groups	1067:1098	polysaccharide functional groups	1067:1098	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses suggested that SIDF had a loose and porous structure, polysaccharide functional groups, and a typical crystalline cellulose I structure.
34369950	2	35	used	utilized	484:491	arg2	Okara					373:377	Okara	373:377	Okara	373:377	Okara is a by-product obtained during soybean processing for soy milk and soybean curd (tofu), which is rarely utilized and can be a cheap potential dietary fiber (DF) resource.
34369950	2	35	used	utilized	484:491	arg2	by-product					384:393	a by-product	382:393	a by-product	382:393	Okara is a by-product obtained during soybean processing for soy milk and soybean curd (tofu), which is rarely utilized and can be a cheap potential dietary fiber (DF) resource.
34369950	0	36	theme	soybean	36:42	arg1	fiber					62:66	soybean insoluble dietary fiber	36:66	soybean insoluble dietary fiber	36:66	Preparation and characterization of soybean insoluble dietary fiber and its prebiotic effect on dyslipidemia and hepatic steatosis in high fat-fed C57BL/6J mice.
34369950	1	37	theme	prospective	284:294	arg1	studies					303:309	large prospective cohort studies	278:309	large prospective cohort studies	278:309	The potential benefits of insoluble dietary fiber (IDF) in the regulation of lipid metabolism have been reported in large prospective cohort studies although the molecular regulatory mechanism is still unclear.
34369950	7	38	theme	fat	1378:1380	arg1	accumulation					1382:1393	hepatic fat accumulation	1370:1393	hepatic fat accumulation	1370:1393	In vivo, IDF supplementation reduced the serum lipid levels and inhibited hepatic fat accumulation.
34369950	5	39	theme	Fourier	927:933	arg1	analyses					1004:1011	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses	891:1011	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses	891:1011	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses suggested that SIDF had a loose and porous structure, polysaccharide functional groups, and a typical crystalline cellulose I structure.
34369950	10	40	from	ingredient	1744:1753	arg1	processing					1763:1772	food processing	1758:1772	food processing	1758:1772	The above results show that SIDF could be used as an ideal functional ingredient in food processing as well as play a positive role in improving the added value of okara and promoting its comprehensive utilization.
34369950	8	41	theme	hepatic	1439:1445	arg1	steatosis					1447:1455	hepatic steatosis	1439:1455	hepatic steatosis	1439:1455	Additionally, SIDF administration improved hepatic steatosis by stimulating lipolysis via upregulation of PPARα, CYP4a10 and CPT1a.
34369950	10	42	used	used	1716:1719	arg2	role					1801:1804	a positive role	1790:1804	a positive role	1790:1804	The above results show that SIDF could be used as an ideal functional ingredient in food processing as well as play a positive role in improving the added value of okara and promoting its comprehensive utilization.
34369950	10	42	used	used	1716:1719	arg2	SIDF					1702:1705	SIDF	1702:1705	SIDF	1702:1705	The above results show that SIDF could be used as an ideal functional ingredient in food processing as well as play a positive role in improving the added value of okara and promoting its comprehensive utilization.
34369950	10	42	used	used	1716:1719	arg2	ingredient					1744:1753	an ideal functional ingredient	1724:1753	an ideal functional ingredient in food processing	1724:1772	The above results show that SIDF could be used as an ideal functional ingredient in food processing as well as play a positive role in improving the added value of okara and promoting its comprehensive utilization.
34369950	0	43	theme	dietary	54:60	arg1	fiber					62:66	soybean insoluble dietary fiber	36:66	soybean insoluble dietary fiber	36:66	Preparation and characterization of soybean insoluble dietary fiber and its prebiotic effect on dyslipidemia and hepatic steatosis in high fat-fed C57BL/6J mice.
34369950	3	44	theme	fiber	632:636	arg1	structure					570:578	structure	570:578	structure	570:578	In this study, the structure and physicochemical properties of insoluble dietary fiber (SIDF) extracted from okara were characterized, and the prebiotic effects on fat metabolism were investigated in vivo.
34369950	3	44	theme	fiber	632:636	arg1	properties					600:609	physicochemical properties	584:609	physicochemical properties	584:609	In this study, the structure and physicochemical properties of insoluble dietary fiber (SIDF) extracted from okara were characterized, and the prebiotic effects on fat metabolism were investigated in vivo.
34369950	8	45	theme	CPT1a	1521:1525	arg1	upregulation					1486:1497	upregulation	1486:1497	upregulation of PPARα, CYP4a10 and CPT1a	1486:1525	Additionally, SIDF administration improved hepatic steatosis by stimulating lipolysis via upregulation of PPARα, CYP4a10 and CPT1a.
34369950	3	46	dep	structure	570:578	arg1	the					566:568	the	566:568	the	566:568	In this study, the structure and physicochemical properties of insoluble dietary fiber (SIDF) extracted from okara were characterized, and the prebiotic effects on fat metabolism were investigated in vivo.
34369950	3	47	theme	insoluble	614:622	arg1	SIDF					639:642	SIDF	639:642	SIDF	639:642	In this study, the structure and physicochemical properties of insoluble dietary fiber (SIDF) extracted from okara were characterized, and the prebiotic effects on fat metabolism were investigated in vivo.
34369950	3	47	theme	insoluble	614:622	arg1	fiber					632:636	insoluble dietary fiber	614:636	insoluble dietary fiber (SIDF) extracted from okara	614:664	In this study, the structure and physicochemical properties of insoluble dietary fiber (SIDF) extracted from okara were characterized, and the prebiotic effects on fat metabolism were investigated in vivo.
34369950	5	48	theme	cellulose	1127:1135	arg1	structure					1139:1147	a typical crystalline cellulose I structure	1105:1147	a typical crystalline cellulose I structure	1105:1147	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses suggested that SIDF had a loose and porous structure, polysaccharide functional groups, and a typical crystalline cellulose I structure.
34369950	7	49	theme	serum	1337:1341	arg1	levels					1349:1354	the serum lipid levels	1333:1354	the serum lipid levels	1333:1354	In vivo, IDF supplementation reduced the serum lipid levels and inhibited hepatic fat accumulation.
34369950	1	50	theme	cohort	296:301	arg1	studies					303:309	large prospective cohort studies	278:309	large prospective cohort studies	278:309	The potential benefits of insoluble dietary fiber (IDF) in the regulation of lipid metabolism have been reported in large prospective cohort studies although the molecular regulatory mechanism is still unclear.
34369950	6	51	theme	ideal	1172:1176	arg1	capacity					1193:1200	ideal oil-adsorption capacity	1172:1200	ideal oil-adsorption capacity (OAC; 7.95 g g-1)	1172:1218	In addition, SIDF had ideal oil-adsorption capacity (OAC; 7.95 g g-1) and significantly improved cholesterol adsorption (11.14 mg g-1) at pH 7.0.
34369950	8	52	theme	PPARα	1502:1506	arg1	upregulation					1486:1497	upregulation	1486:1497	upregulation of PPARα, CYP4a10 and CPT1a	1486:1525	Additionally, SIDF administration improved hepatic steatosis by stimulating lipolysis via upregulation of PPARα, CYP4a10 and CPT1a.
34369950	10	53	theme	food	1758:1761	arg1	processing					1763:1772	food processing	1758:1772	food processing	1758:1772	The above results show that SIDF could be used as an ideal functional ingredient in food processing as well as play a positive role in improving the added value of okara and promoting its comprehensive utilization.
34369950	1	54	theme	metabolism	245:254	arg1	regulation					225:234	the regulation	221:234	the regulation of lipid metabolism	221:254	The potential benefits of insoluble dietary fiber (IDF) in the regulation of lipid metabolism have been reported in large prospective cohort studies although the molecular regulatory mechanism is still unclear.
34369950	7	55	theme	IDF	1305:1307	arg1	supplementation					1309:1323	IDF supplementation	1305:1323	IDF supplementation	1305:1323	In vivo, IDF supplementation reduced the serum lipid levels and inhibited hepatic fat accumulation.
34369950	8	56	theme	CYP4a10	1509:1515	arg1	upregulation					1486:1497	upregulation	1486:1497	upregulation of PPARα, CYP4a10 and CPT1a	1486:1525	Additionally, SIDF administration improved hepatic steatosis by stimulating lipolysis via upregulation of PPARα, CYP4a10 and CPT1a.
34369950	1	57	theme	potential	166:174	arg1	benefits					176:183	The potential benefits	162:183	The potential benefits of insoluble dietary fiber (IDF) in the regulation of lipid metabolism	162:254	The potential benefits of insoluble dietary fiber (IDF) in the regulation of lipid metabolism have been reported in large prospective cohort studies although the molecular regulatory mechanism is still unclear.
34369950	2	58	dep	cheap	506:510	arg1	fiber					530:534	potential dietary fiber	512:534	potential dietary fiber (DF)	512:539	Okara is a by-product obtained during soybean processing for soy milk and soybean curd (tofu), which is rarely utilized and can be a cheap potential dietary fiber (DF) resource.
34369950	2	58	dep	cheap	506:510	arg1	DF					537:538	DF	537:538	DF	537:538	Okara is a by-product obtained during soybean processing for soy milk and soybean curd (tofu), which is rarely utilized and can be a cheap potential dietary fiber (DF) resource.
34369950	9	59	theme	first	1540:1544	arg1	This					1528:1531	This	1528:1531	This	1528:1531	This is the first systematic study on the composition, structure, physicochemical properties, adsorption function and biological effects of SIDF.
34369950	9	59	theme	first	1540:1544	arg1	study					1557:1561	the first systematic study	1536:1561	the first systematic study on the composition, structure, physicochemical properties, adsorption function and biological effects of SIDF	1536:1671	This is the first systematic study on the composition, structure, physicochemical properties, adsorption function and biological effects of SIDF.
34369950	6	60	theme	cholesterol	1247:1257	arg1	adsorption					1259:1268	significantly improved cholesterol adsorption	1224:1268	significantly improved cholesterol adsorption (11.14 mg g-1)	1224:1283	In addition, SIDF had ideal oil-adsorption capacity (OAC; 7.95 g g-1) and significantly improved cholesterol adsorption (11.14 mg g-1) at pH 7.0.
34369950	6	60	theme	cholesterol	1247:1257	arg1	g-1					1280:1282	11.14 mg g-1	1271:1282	11.14 mg g-1	1271:1282	In addition, SIDF had ideal oil-adsorption capacity (OAC; 7.95 g g-1) and significantly improved cholesterol adsorption (11.14 mg g-1) at pH 7.0.
34369950	4	61	theme	main	785:788	arg1	%					820:820	90.50%	815:820	90.50%	815:820	The results showed that the main monosaccharides of SIDF (90.50%) identified were galactose, arabinose, xylose, rhamnose and glucose.
34369950	4	61	theme	main	785:788	arg1	monosaccharides					790:804	the main monosaccharides	781:804	the main monosaccharides of SIDF (90.50%) identified	781:832	The results showed that the main monosaccharides of SIDF (90.50%) identified were galactose, arabinose, xylose, rhamnose and glucose.
34369950	4	61	theme	main	785:788	arg1	galactose					839:847	galactose	839:847	galactose	839:847	The results showed that the main monosaccharides of SIDF (90.50%) identified were galactose, arabinose, xylose, rhamnose and glucose.
34369950	5	62	dep	Fourier	927:933	arg1	transform					935:943	transform	935:943	transform infrared spectroscopy (FT-IR)	935:973	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses suggested that SIDF had a loose and porous structure, polysaccharide functional groups, and a typical crystalline cellulose I structure.
34369950	3	63	theme	prebiotic	694:702	arg1	effects					704:710	the prebiotic effects	690:710	the prebiotic effects on fat metabolism	690:728	In this study, the structure and physicochemical properties of insoluble dietary fiber (SIDF) extracted from okara were characterized, and the prebiotic effects on fat metabolism were investigated in vivo.
34369950	1	64	theme	dietary	198:204	arg1	fiber					206:210	insoluble dietary fiber	188:210	insoluble dietary fiber (IDF)	188:216	The potential benefits of insoluble dietary fiber (IDF) in the regulation of lipid metabolism have been reported in large prospective cohort studies although the molecular regulatory mechanism is still unclear.
34369950	1	64	theme	dietary	198:204	arg1	IDF					213:215	IDF	213:215	IDF	213:215	The potential benefits of insoluble dietary fiber (IDF) in the regulation of lipid metabolism have been reported in large prospective cohort studies although the molecular regulatory mechanism is still unclear.
34369950	2	65	theme	cheap	506:510	arg1	resource					541:548	a cheap potential dietary fiber (DF) resource	504:548	a cheap potential dietary fiber (DF) resource	504:548	Okara is a by-product obtained during soybean processing for soy milk and soybean curd (tofu), which is rarely utilized and can be a cheap potential dietary fiber (DF) resource.
34369950	0	66	from	effect	86:91	arg1	dyslipidemia					96:107	dyslipidemia	96:107	dyslipidemia	96:107	Preparation and characterization of soybean insoluble dietary fiber and its prebiotic effect on dyslipidemia and hepatic steatosis in high fat-fed C57BL/6J mice.
34369950	0	66	from	effect	86:91	arg1	steatosis					121:129	hepatic steatosis	113:129	hepatic steatosis	113:129	Preparation and characterization of soybean insoluble dietary fiber and its prebiotic effect on dyslipidemia and hepatic steatosis in high fat-fed C57BL/6J mice.
34369950	0	67	theme	fat-fed	139:145	arg1	mice					156:159	high fat-fed C57BL/6J mice	134:159	high fat-fed C57BL/6J mice	134:159	Preparation and characterization of soybean insoluble dietary fiber and its prebiotic effect on dyslipidemia and hepatic steatosis in high fat-fed C57BL/6J mice.
34369950	5	68	theme	diffraction	986:996	arg1	analyses					1004:1011	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses	891:1011	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses	891:1011	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses suggested that SIDF had a loose and porous structure, polysaccharide functional groups, and a typical crystalline cellulose I structure.
34369950	10	69	theme	added	1823:1827	arg1	value					1829:1833	the added value	1819:1833	the added value of okara	1819:1842	The above results show that SIDF could be used as an ideal functional ingredient in food processing as well as play a positive role in improving the added value of okara and promoting its comprehensive utilization.
34369950	2	70	theme	dietary	522:528	arg1	fiber					530:534	potential dietary fiber	512:534	potential dietary fiber (DF)	512:539	Okara is a by-product obtained during soybean processing for soy milk and soybean curd (tofu), which is rarely utilized and can be a cheap potential dietary fiber (DF) resource.
34369950	2	70	theme	dietary	522:528	arg1	DF					537:538	DF	537:538	DF	537:538	Okara is a by-product obtained during soybean processing for soy milk and soybean curd (tofu), which is rarely utilized and can be a cheap potential dietary fiber (DF) resource.
34369950	0	71	from	dyslipidemia	96:107	arg1	mice					156:159	high fat-fed C57BL/6J mice	134:159	high fat-fed C57BL/6J mice	134:159	Preparation and characterization of soybean insoluble dietary fiber and its prebiotic effect on dyslipidemia and hepatic steatosis in high fat-fed C57BL/6J mice.
34369950	6	72	theme	11.14	1271:1275	arg1	mg					1277:1278	mg	1277:1278	mg	1277:1278	In addition, SIDF had ideal oil-adsorption capacity (OAC; 7.95 g g-1) and significantly improved cholesterol adsorption (11.14 mg g-1) at pH 7.0.
34369950	9	73	theme	adsorption	1622:1631	arg1	function					1633:1640	adsorption function	1622:1640	adsorption function	1622:1640	This is the first systematic study on the composition, structure, physicochemical properties, adsorption function and biological effects of SIDF.
34369950	5	74	theme	typical	1107:1113	arg1	structure					1139:1147	a typical crystalline cellulose I structure	1105:1147	a typical crystalline cellulose I structure	1105:1147	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses suggested that SIDF had a loose and porous structure, polysaccharide functional groups, and a typical crystalline cellulose I structure.
34369950	1	75	theme	molecular	324:332	arg1	mechanism					345:353	the molecular regulatory mechanism	320:353	the molecular regulatory mechanism	320:353	The potential benefits of insoluble dietary fiber (IDF) in the regulation of lipid metabolism have been reported in large prospective cohort studies although the molecular regulatory mechanism is still unclear.
34369950	1	75	theme	molecular	324:332	arg1	unclear					364:370	unclear	364:370	unclear	364:370	The potential benefits of insoluble dietary fiber (IDF) in the regulation of lipid metabolism have been reported in large prospective cohort studies although the molecular regulatory mechanism is still unclear.
34369950	1	76	from	benefits	176:183	arg1	regulation					225:234	the regulation	221:234	the regulation of lipid metabolism	221:254	The potential benefits of insoluble dietary fiber (IDF) in the regulation of lipid metabolism have been reported in large prospective cohort studies although the molecular regulatory mechanism is still unclear.
34369950	6	77	theme	improved	1238:1245	arg1	adsorption					1259:1268	significantly improved cholesterol adsorption	1224:1268	significantly improved cholesterol adsorption (11.14 mg g-1)	1224:1283	In addition, SIDF had ideal oil-adsorption capacity (OAC; 7.95 g g-1) and significantly improved cholesterol adsorption (11.14 mg g-1) at pH 7.0.
34369950	6	77	theme	improved	1238:1245	arg1	g-1					1280:1282	11.14 mg g-1	1271:1282	11.14 mg g-1	1271:1282	In addition, SIDF had ideal oil-adsorption capacity (OAC; 7.95 g g-1) and significantly improved cholesterol adsorption (11.14 mg g-1) at pH 7.0.
34369950	0	78	theme	hepatic	113:119	arg1	steatosis					121:129	hepatic steatosis	113:129	hepatic steatosis	113:129	Preparation and characterization of soybean insoluble dietary fiber and its prebiotic effect on dyslipidemia and hepatic steatosis in high fat-fed C57BL/6J mice.
34369950	5	79	theme	Scanning	891:898	arg1	SEM					921:923	SEM	921:923	SEM	921:923	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses suggested that SIDF had a loose and porous structure, polysaccharide functional groups, and a typical crystalline cellulose I structure.
34369950	5	79	theme	Scanning	891:898	arg1	microscopy					909:918	Scanning electron microscopy	891:918	Scanning electron microscopy (SEM)	891:924	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses suggested that SIDF had a loose and porous structure, polysaccharide functional groups, and a typical crystalline cellulose I structure.
34369950	9	80	theme	SIDF	1668:1671	arg1	composition					1570:1580	composition	1570:1580	composition	1570:1580	This is the first systematic study on the composition, structure, physicochemical properties, adsorption function and biological effects of SIDF.
34369950	9	80	theme	SIDF	1668:1671	arg1	effects					1657:1663	biological effects	1646:1663	biological effects	1646:1663	This is the first systematic study on the composition, structure, physicochemical properties, adsorption function and biological effects of SIDF.
34369950	9	80	theme	SIDF	1668:1671	arg1	function					1633:1640	adsorption function	1622:1640	adsorption function	1622:1640	This is the first systematic study on the composition, structure, physicochemical properties, adsorption function and biological effects of SIDF.
34369950	9	80	theme	SIDF	1668:1671	arg1	structure					1583:1591	structure	1583:1591	structure	1583:1591	This is the first systematic study on the composition, structure, physicochemical properties, adsorption function and biological effects of SIDF.
34369950	9	80	theme	SIDF	1668:1671	arg1	properties					1610:1619	physicochemical properties	1594:1619	physicochemical properties	1594:1619	This is the first systematic study on the composition, structure, physicochemical properties, adsorption function and biological effects of SIDF.
34369950	8	81	theme	SIDF	1410:1413	arg1	administration					1415:1428	SIDF administration	1410:1428	SIDF administration	1410:1428	Additionally, SIDF administration improved hepatic steatosis by stimulating lipolysis via upregulation of PPARα, CYP4a10 and CPT1a.
34369950	10	82	theme	ideal	1727:1731	arg1	SIDF					1702:1705	SIDF	1702:1705	SIDF	1702:1705	The above results show that SIDF could be used as an ideal functional ingredient in food processing as well as play a positive role in improving the added value of okara and promoting its comprehensive utilization.
34369950	10	82	theme	ideal	1727:1731	arg1	role					1801:1804	a positive role	1790:1804	a positive role	1790:1804	The above results show that SIDF could be used as an ideal functional ingredient in food processing as well as play a positive role in improving the added value of okara and promoting its comprehensive utilization.
34369950	10	82	theme	ideal	1727:1731	arg1	ingredient					1744:1753	an ideal functional ingredient	1724:1753	an ideal functional ingredient in food processing	1724:1772	The above results show that SIDF could be used as an ideal functional ingredient in food processing as well as play a positive role in improving the added value of okara and promoting its comprehensive utilization.
34369950	3	83	theme	physicochemical	584:598	arg1	properties					600:609	physicochemical properties	584:609	physicochemical properties	584:609	In this study, the structure and physicochemical properties of insoluble dietary fiber (SIDF) extracted from okara were characterized, and the prebiotic effects on fat metabolism were investigated in vivo.
34369950	2	84	theme	soybean	411:417	arg1	processing					419:428	soybean processing	411:428	soybean processing for soy milk and soybean curd (tofu)	411:465	Okara is a by-product obtained during soybean processing for soy milk and soybean curd (tofu), which is rarely utilized and can be a cheap potential dietary fiber (DF) resource.
34369950	0	85	theme	insoluble	44:52	arg1	fiber					62:66	soybean insoluble dietary fiber	36:66	soybean insoluble dietary fiber	36:66	Preparation and characterization of soybean insoluble dietary fiber and its prebiotic effect on dyslipidemia and hepatic steatosis in high fat-fed C57BL/6J mice.
34369950	1	86	theme	large	278:282	arg1	studies					303:309	large prospective cohort studies	278:309	large prospective cohort studies	278:309	The potential benefits of insoluble dietary fiber (IDF) in the regulation of lipid metabolism have been reported in large prospective cohort studies although the molecular regulatory mechanism is still unclear.
34369950	0	87	theme	fiber	62:66	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of soybean insoluble dietary fiber and its prebiotic effect on dyslipidemia and hepatic steatosis in high fat-fed C57BL/6J mice.
34369950	0	87	theme	fiber	62:66	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of soybean insoluble dietary fiber and its prebiotic effect on dyslipidemia and hepatic steatosis in high fat-fed C57BL/6J mice.
34369950	0	87	theme	fiber	62:66	arg1	effect					86:91	its prebiotic effect	72:91	its prebiotic effect on dyslipidemia and hepatic steatosis in high fat-fed C57BL/6J mice	72:159	Preparation and characterization of soybean insoluble dietary fiber and its prebiotic effect on dyslipidemia and hepatic steatosis in high fat-fed C57BL/6J mice.
34369950	5	88	theme	loose	1039:1043	arg1	structure					1056:1064	a loose and porous structure	1037:1064	structure	1056:1064	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses suggested that SIDF had a loose and porous structure, polysaccharide functional groups, and a typical crystalline cellulose I structure.
34369950	2	89	theme	soybean	447:453	arg1	curd					455:458	soybean curd	447:458	soybean curd (tofu)	447:465	Okara is a by-product obtained during soybean processing for soy milk and soybean curd (tofu), which is rarely utilized and can be a cheap potential dietary fiber (DF) resource.
34369950	2	89	theme	soybean	447:453	arg1	tofu					461:464	tofu	461:464	tofu	461:464	Okara is a by-product obtained during soybean processing for soy milk and soybean curd (tofu), which is rarely utilized and can be a cheap potential dietary fiber (DF) resource.
34369950	3	90	theme	dietary	624:630	arg1	SIDF					639:642	SIDF	639:642	SIDF	639:642	In this study, the structure and physicochemical properties of insoluble dietary fiber (SIDF) extracted from okara were characterized, and the prebiotic effects on fat metabolism were investigated in vivo.
34369950	3	90	theme	dietary	624:630	arg1	fiber					632:636	insoluble dietary fiber	614:636	insoluble dietary fiber (SIDF) extracted from okara	614:664	In this study, the structure and physicochemical properties of insoluble dietary fiber (SIDF) extracted from okara were characterized, and the prebiotic effects on fat metabolism were investigated in vivo.
34369950	5	91	theme	porous	1049:1054	arg1	structure					1056:1064	a loose and porous structure	1037:1064	structure	1056:1064	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses suggested that SIDF had a loose and porous structure, polysaccharide functional groups, and a typical crystalline cellulose I structure.
34369950	7	92	theme	hepatic	1370:1376	arg1	accumulation					1382:1393	hepatic fat accumulation	1370:1393	hepatic fat accumulation	1370:1393	In vivo, IDF supplementation reduced the serum lipid levels and inhibited hepatic fat accumulation.
34369950	5	93	theme	microscopy	909:918	arg1	analyses					1004:1011	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses	891:1011	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses	891:1011	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses suggested that SIDF had a loose and porous structure, polysaccharide functional groups, and a typical crystalline cellulose I structure.
34369950	10	94	theme	functional	1733:1742	arg1	SIDF					1702:1705	SIDF	1702:1705	SIDF	1702:1705	The above results show that SIDF could be used as an ideal functional ingredient in food processing as well as play a positive role in improving the added value of okara and promoting its comprehensive utilization.
34369950	10	94	theme	functional	1733:1742	arg1	role					1801:1804	a positive role	1790:1804	a positive role	1790:1804	The above results show that SIDF could be used as an ideal functional ingredient in food processing as well as play a positive role in improving the added value of okara and promoting its comprehensive utilization.
34369950	10	94	theme	functional	1733:1742	arg1	ingredient					1744:1753	an ideal functional ingredient	1724:1753	an ideal functional ingredient in food processing	1724:1772	The above results show that SIDF could be used as an ideal functional ingredient in food processing as well as play a positive role in improving the added value of okara and promoting its comprehensive utilization.
34369950	5	95	theme	crystalline	1115:1125	arg1	structure					1139:1147	a typical crystalline cellulose I structure	1105:1147	a typical crystalline cellulose I structure	1105:1147	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses suggested that SIDF had a loose and porous structure, polysaccharide functional groups, and a typical crystalline cellulose I structure.
34369950	6	96	theme	mg	1277:1278	arg1	adsorption					1259:1268	significantly improved cholesterol adsorption	1224:1268	significantly improved cholesterol adsorption (11.14 mg g-1)	1224:1283	In addition, SIDF had ideal oil-adsorption capacity (OAC; 7.95 g g-1) and significantly improved cholesterol adsorption (11.14 mg g-1) at pH 7.0.
34369950	6	96	theme	mg	1277:1278	arg1	g-1					1280:1282	11.14 mg g-1	1271:1282	11.14 mg g-1	1271:1282	In addition, SIDF had ideal oil-adsorption capacity (OAC; 7.95 g g-1) and significantly improved cholesterol adsorption (11.14 mg g-1) at pH 7.0.
34369950	0	97	from	steatosis	121:129	arg1	mice					156:159	high fat-fed C57BL/6J mice	134:159	high fat-fed C57BL/6J mice	134:159	Preparation and characterization of soybean insoluble dietary fiber and its prebiotic effect on dyslipidemia and hepatic steatosis in high fat-fed C57BL/6J mice.
34369950	5	98	theme	I	1137:1137	arg1	structure					1139:1147	a typical crystalline cellulose I structure	1105:1147	a typical crystalline cellulose I structure	1105:1147	Scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analyses suggested that SIDF had a loose and porous structure, polysaccharide functional groups, and a typical crystalline cellulose I structure.
33970399	9	0	theme	stents	1519:1524	arg1	rates					1506:1510	the biodegradation rates	1487:1510	the biodegradation rates of the stents	1487:1524	An addition of nanofibers to alginate hydrogel significantly enhanced the biodegradation rates of the stents.
33970399	3	1	theme	head	584:587	arg1	nozzle					589:594	an 22G print head nozzle	571:594	an 22G print head nozzle attached to the BD-syringe	571:621	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	2	theme	w/v	440:442	arg1	resources					397:405	hydrogel (~5 ml) resources	380:405	hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe	380:621	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	2	theme	w/v	440:442	arg1	SA-CYS					470:475	SA-CYS	470:475	SA-CYS	470:475	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	2	theme	w/v	440:442	arg1	alginate					460:467	10%w/v cysteine-sodium alginate	437:467	10%w/v cysteine-sodium alginate (SA-CYS)	437:476	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	2	3	theme	printing	218:225	arg1	technique					227:235	Three-dimensional (3D) printing technique	195:235	Three-dimensional (3D) printing technique	195:235	Three-dimensional (3D) printing technique has offered easy and fast fabrication of BDS with enhanced reproducibility and efficacy.
33970399	3	4	with	alginate	460:467	arg1	SA-CYS-NF					544:552	SA-CYS-NF	544:552	SA-CYS-NF	544:552	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	4	with	alginate	460:467	arg1	PLA-nanofibers					528:541	0.4%w/v PLA-nanofibers	520:541	0.4%w/v PLA-nanofibers (SA-CYS-NF)	520:553	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	2	5	theme	BDS	278:280	arg1	fabrication					263:273	easy and fast fabrication	249:273	easy and fast fabrication of BDS with enhanced reproducibility and efficacy	249:323	Three-dimensional (3D) printing technique has offered easy and fast fabrication of BDS with enhanced reproducibility and efficacy.
33970399	4	6	theme	printing	735:742	arg1	distance					744:751	printing distance	735:751	printing distance	735:751	The printability of hydrogels into 3D structures was examined based on such variables as hydrogel's viscosity, printing distance, printing speed and the nozzle size.
33970399	10	7	theme	In	1527:1528	arg1	studies					1549:1555	In vitro cell culture studies	1527:1555	In vitro cell culture studies	1527:1555	In vitro cell culture studies revealed that stents had no cytotoxic effects on human umbilical vein endothelial cells (HUVECs) and Raw 264.7 cells (i.e., Monocyte/macrophage-like cells), supporting that stents are biocompatible and can be explored for future clinical applications.
33970399	10	8	theme	clinical	1786:1793	arg1	applications					1795:1806	future clinical applications	1779:1806	future clinical applications	1779:1806	In vitro cell culture studies revealed that stents had no cytotoxic effects on human umbilical vein endothelial cells (HUVECs) and Raw 264.7 cells (i.e., Monocyte/macrophage-like cells), supporting that stents are biocompatible and can be explored for future clinical applications.
33970399	10	9	theme	umbilical	1612:1620	arg1	HUVECs					1646:1651	HUVECs	1646:1651	HUVECs	1646:1651	In vitro cell culture studies revealed that stents had no cytotoxic effects on human umbilical vein endothelial cells (HUVECs) and Raw 264.7 cells (i.e., Monocyte/macrophage-like cells), supporting that stents are biocompatible and can be explored for future clinical applications.
33970399	10	9	theme	umbilical	1612:1620	arg1	cells					1639:1643	human umbilical vein endothelial cells	1606:1643	human umbilical vein endothelial cells (HUVECs)	1606:1652	In vitro cell culture studies revealed that stents had no cytotoxic effects on human umbilical vein endothelial cells (HUVECs) and Raw 264.7 cells (i.e., Monocyte/macrophage-like cells), supporting that stents are biocompatible and can be explored for future clinical applications.
33970399	1	10	theme	coronary	171:178	arg1	disease					186:192	coronary heart disease	171:192	coronary heart disease	171:192	PURPOSE This study aimed to develop personalized biodegradable stent (BDS) for the treatment of coronary heart disease.
33970399	10	11	dep	cells	1706:1710	arg1	i.e.					1675:1678	i.e.	1675:1678	i.e.	1675:1678	In vitro cell culture studies revealed that stents had no cytotoxic effects on human umbilical vein endothelial cells (HUVECs) and Raw 264.7 cells (i.e., Monocyte/macrophage-like cells), supporting that stents are biocompatible and can be explored for future clinical applications.
33970399	3	12	theme	w/v	486:488	arg1	resources					397:405	hydrogel (~5 ml) resources	380:405	hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe	380:621	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	12	theme	w/v	486:488	arg1	alginate					506:513	10%w/v cysteine-sodium alginate	483:513	10%w/v cysteine-sodium alginate	483:513	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	1	13	theme	disease	186:192	arg1	treatment					158:166	the treatment	154:166	the treatment of coronary heart disease	154:192	PURPOSE This study aimed to develop personalized biodegradable stent (BDS) for the treatment of coronary heart disease.
33970399	2	14	theme	enhanced	287:294	arg1	reproducibility					296:310	reproducibility	296:310	reproducibility	296:310	Three-dimensional (3D) printing technique has offered easy and fast fabrication of BDS with enhanced reproducibility and efficacy.
33970399	10	15	contain	had	1578:1580	arg2	effects					1595:1601	no cytotoxic effects	1582:1601	no cytotoxic effects	1582:1601	In vitro cell culture studies revealed that stents had no cytotoxic effects on human umbilical vein endothelial cells (HUVECs) and Raw 264.7 cells (i.e., Monocyte/macrophage-like cells), supporting that stents are biocompatible and can be explored for future clinical applications.
33970399	10	15	contain	had	1578:1580	arg1	stents					1571:1576	stents	1571:1576	stents	1571:1576	In vitro cell culture studies revealed that stents had no cytotoxic effects on human umbilical vein endothelial cells (HUVECs) and Raw 264.7 cells (i.e., Monocyte/macrophage-like cells), supporting that stents are biocompatible and can be explored for future clinical applications.
33970399	5	16	theme	struts	950:955	arg1	ratio					931:935	the thickness and swelling ratio	904:935	the thickness and swelling ratio of the stent struts	904:955	RESULTS It was demonstrated that alginate composition (10%w/v) offered BDS with sufficient viscosity that defined the thickness and swelling ratio of the stent struts.
33970399	3	17	theme	0.4	520:522	arg1	%					523:523	%	523:523	%	523:523	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	10	18	theme	Raw	1658:1660	arg1	cells					1668:1672	Raw 264.7 cells	1658:1672	Raw 264.7 cells (i.e., Monocyte/macrophage-like cells)	1658:1711	In vitro cell culture studies revealed that stents had no cytotoxic effects on human umbilical vein endothelial cells (HUVECs) and Raw 264.7 cells (i.e., Monocyte/macrophage-like cells), supporting that stents are biocompatible and can be explored for future clinical applications.
33970399	10	18	theme	Raw	1658:1660	arg1	cells					1706:1710	Monocyte/macrophage-like cells	1681:1710	Monocyte/macrophage-like cells	1681:1710	In vitro cell culture studies revealed that stents had no cytotoxic effects on human umbilical vein endothelial cells (HUVECs) and Raw 264.7 cells (i.e., Monocyte/macrophage-like cells), supporting that stents are biocompatible and can be explored for future clinical applications.
33970399	10	19	theme	Monocyte/macrophage-like	1681:1704	arg1	cells					1668:1672	Raw 264.7 cells	1658:1672	Raw 264.7 cells (i.e., Monocyte/macrophage-like cells)	1658:1711	In vitro cell culture studies revealed that stents had no cytotoxic effects on human umbilical vein endothelial cells (HUVECs) and Raw 264.7 cells (i.e., Monocyte/macrophage-like cells), supporting that stents are biocompatible and can be explored for future clinical applications.
33970399	10	19	theme	Monocyte/macrophage-like	1681:1704	arg1	cells					1706:1710	Monocyte/macrophage-like cells	1681:1710	Monocyte/macrophage-like cells	1681:1710	In vitro cell culture studies revealed that stents had no cytotoxic effects on human umbilical vein endothelial cells (HUVECs) and Raw 264.7 cells (i.e., Monocyte/macrophage-like cells), supporting that stents are biocompatible and can be explored for future clinical applications.
33970399	8	20	theme	sufficient	1322:1331	arg1	strength					1344:1351	sufficient mechanical strength	1322:1351	sufficient mechanical strength	1322:1351	CONCLUSION The printed stents had sufficient mechanical strength and were stable against pseudo-physiological wall shear stress.
33970399	3	21	theme	w/v	524:526	arg1	SA-CYS-NF					544:552	SA-CYS-NF	544:552	SA-CYS-NF	544:552	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	21	theme	w/v	524:526	arg1	PLA-nanofibers					528:541	0.4%w/v PLA-nanofibers	520:541	0.4%w/v PLA-nanofibers (SA-CYS-NF)	520:553	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	2	22	with	BDS	278:280	arg1	reproducibility					296:310	reproducibility	296:310	reproducibility	296:310	Three-dimensional (3D) printing technique has offered easy and fast fabrication of BDS with enhanced reproducibility and efficacy.
33970399	2	22	with	BDS	278:280	arg1	efficacy					316:323	efficacy	316:323	efficacy	316:323	Three-dimensional (3D) printing technique has offered easy and fast fabrication of BDS with enhanced reproducibility and efficacy.
33970399	10	23	theme	cytotoxic	1585:1593	arg1	effects					1595:1601	no cytotoxic effects	1582:1601	no cytotoxic effects	1582:1601	In vitro cell culture studies revealed that stents had no cytotoxic effects on human umbilical vein endothelial cells (HUVECs) and Raw 264.7 cells (i.e., Monocyte/macrophage-like cells), supporting that stents are biocompatible and can be explored for future clinical applications.
33970399	9	24	theme	alginate	1446:1453	arg1	hydrogel					1455:1462	alginate hydrogel	1446:1462	alginate hydrogel	1446:1462	An addition of nanofibers to alginate hydrogel significantly enhanced the biodegradation rates of the stents.
33970399	3	25	with	alginate	506:513	arg1	SA-CYS-NF					544:552	SA-CYS-NF	544:552	SA-CYS-NF	544:552	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	25	with	alginate	506:513	arg1	PLA-nanofibers					528:541	0.4%w/v PLA-nanofibers	520:541	0.4%w/v PLA-nanofibers (SA-CYS-NF)	520:553	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	10	26	theme	endothelial	1627:1637	arg1	HUVECs					1646:1651	HUVECs	1646:1651	HUVECs	1646:1651	In vitro cell culture studies revealed that stents had no cytotoxic effects on human umbilical vein endothelial cells (HUVECs) and Raw 264.7 cells (i.e., Monocyte/macrophage-like cells), supporting that stents are biocompatible and can be explored for future clinical applications.
33970399	10	26	theme	endothelial	1627:1637	arg1	cells					1639:1643	human umbilical vein endothelial cells	1606:1643	human umbilical vein endothelial cells (HUVECs)	1606:1652	In vitro cell culture studies revealed that stents had no cytotoxic effects on human umbilical vein endothelial cells (HUVECs) and Raw 264.7 cells (i.e., Monocyte/macrophage-like cells), supporting that stents are biocompatible and can be explored for future clinical applications.
33970399	8	27	theme	wall	1398:1401	arg1	stress					1409:1414	pseudo-physiological wall shear stress	1377:1414	pseudo-physiological wall shear stress	1377:1414	CONCLUSION The printed stents had sufficient mechanical strength and were stable against pseudo-physiological wall shear stress.
33970399	3	28	theme	resources	397:405	arg1	types					371:375	3 types	369:375	3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe	369:621	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	0	29	theme	Parametric	0:9	arg1	Optimization					11:22	Parametric Optimization	0:22	Parametric Optimization of 3D Printed Hydrogel-Based Cardiovascular Stent.	0:73	Parametric Optimization of 3D Printed Hydrogel-Based Cardiovascular Stent.
33970399	9	30	theme	nanofibers	1432:1441	arg1	addition					1420:1427	An addition	1417:1427	An addition of nanofibers to alginate hydrogel	1417:1462	An addition of nanofibers to alginate hydrogel significantly enhanced the biodegradation rates of the stents.
33970399	2	31	theme	easy	249:252	arg1	fabrication					263:273	easy and fast fabrication	249:273	easy and fast fabrication of BDS with enhanced reproducibility and efficacy	249:323	Three-dimensional (3D) printing technique has offered easy and fast fabrication of BDS with enhanced reproducibility and efficacy.
33970399	1	32	theme	personalized	111:122	arg1	BDS					145:147	BDS	145:147	BDS	145:147	PURPOSE This study aimed to develop personalized biodegradable stent (BDS) for the treatment of coronary heart disease.
33970399	1	32	theme	personalized	111:122	arg1	stent					138:142	personalized biodegradable stent	111:142	personalized biodegradable stent (BDS) for the treatment of coronary heart disease	111:192	PURPOSE This study aimed to develop personalized biodegradable stent (BDS) for the treatment of coronary heart disease.
33970399	7	33	theme	%	1179:1179	arg1	ratio					1160:1164	the highest swelling ratio	1139:1164	the highest swelling ratio of 38.8 ± 2.9%	1139:1179	SA-CYS-NF stent displayed the highest swelling ratio of 38.8 ± 2.9% at the initial 30 min, whereas stents made of SA and SA-CYS had 23.1 ± 2.4% and 22.0 ± 2.4%, respectively.
33970399	3	34	theme	~5 ml	390:394	arg1	resources					397:405	hydrogel (~5 ml) resources	380:405	hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe	380:621	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	34	theme	~5 ml	390:394	arg1	alginate					422:429	10%w/v sodium alginate	408:429	10%w/v sodium alginate (SA)	408:434	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	34	theme	~5 ml	390:394	arg1	alginate					506:513	10%w/v cysteine-sodium alginate	483:513	10%w/v cysteine-sodium alginate	483:513	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	34	theme	~5 ml	390:394	arg1	alginate					460:467	10%w/v cysteine-sodium alginate	437:467	10%w/v cysteine-sodium alginate (SA-CYS)	437:476	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	35	theme	w/v	411:413	arg1	SA					432:433	SA	432:433	SA	432:433	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	35	theme	w/v	411:413	arg1	alginate					422:429	10%w/v sodium alginate	408:429	10%w/v sodium alginate (SA)	408:434	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	35	theme	w/v	411:413	arg1	resources					397:405	hydrogel (~5 ml) resources	380:405	hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe	380:621	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	10	36	theme	culture	1541:1547	arg1	studies					1549:1555	In vitro cell culture studies	1527:1555	In vitro cell culture studies	1527:1555	In vitro cell culture studies revealed that stents had no cytotoxic effects on human umbilical vein endothelial cells (HUVECs) and Raw 264.7 cells (i.e., Monocyte/macrophage-like cells), supporting that stents are biocompatible and can be explored for future clinical applications.
33970399	0	37	theme	Printed	30:36	arg1	Stent					68:72	3D Printed Hydrogel-Based Cardiovascular Stent	27:72	3D Printed Hydrogel-Based Cardiovascular Stent	27:72	Parametric Optimization of 3D Printed Hydrogel-Based Cardiovascular Stent.
33970399	3	38	theme	hydrogel	380:387	arg1	resources					397:405	hydrogel (~5 ml) resources	380:405	hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe	380:621	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	38	theme	hydrogel	380:387	arg1	alginate					422:429	10%w/v sodium alginate	408:429	10%w/v sodium alginate (SA)	408:434	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	38	theme	hydrogel	380:387	arg1	alginate					506:513	10%w/v cysteine-sodium alginate	483:513	10%w/v cysteine-sodium alginate	483:513	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	38	theme	hydrogel	380:387	arg1	alginate					460:467	10%w/v cysteine-sodium alginate	437:467	10%w/v cysteine-sodium alginate (SA-CYS)	437:476	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	0	39	theme	Cardiovascular	53:66	arg1	Stent					68:72	3D Printed Hydrogel-Based Cardiovascular Stent	27:72	3D Printed Hydrogel-Based Cardiovascular Stent	27:72	Parametric Optimization of 3D Printed Hydrogel-Based Cardiovascular Stent.
33970399	8	40	dep	CONCLUSION	1288:1297	arg1	had					1318:1320	had	1318:1320	had sufficient mechanical strength	1318:1351	CONCLUSION The printed stents had sufficient mechanical strength and were stable against pseudo-physiological wall shear stress.
33970399	8	40	dep	CONCLUSION	1288:1297	arg1	stable					1362:1367	stable	1362:1367	stable	1362:1367	CONCLUSION The printed stents had sufficient mechanical strength and were stable against pseudo-physiological wall shear stress.
33970399	8	40	dep	CONCLUSION	1288:1297	arg1	stents					1311:1316	The printed stents	1299:1316	The printed stents	1299:1316	CONCLUSION The printed stents had sufficient mechanical strength and were stable against pseudo-physiological wall shear stress.
33970399	5	41	theme	thickness	908:916	arg1	ratio					931:935	the thickness and swelling ratio	904:935	the thickness and swelling ratio of the stent struts	904:955	RESULTS It was demonstrated that alginate composition (10%w/v) offered BDS with sufficient viscosity that defined the thickness and swelling ratio of the stent struts.
33970399	7	42	theme	swelling	1151:1158	arg1	ratio					1160:1164	the highest swelling ratio	1139:1164	the highest swelling ratio of 38.8 ± 2.9%	1139:1179	SA-CYS-NF stent displayed the highest swelling ratio of 38.8 ± 2.9% at the initial 30 min, whereas stents made of SA and SA-CYS had 23.1 ± 2.4% and 22.0 ± 2.4%, respectively.
33970399	3	43	theme	print	578:582	arg1	nozzle					589:594	an 22G print head nozzle	571:594	an 22G print head nozzle attached to the BD-syringe	571:621	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	6	44	theme	strut	979:983	arg1	thickness					962:970	The thickness	958:970	The thickness of the strut	958:983	The thickness of the strut was found to be 338.7 ± 29.3 μm, 262.5 ± 14.7 μm and 237.1 ± 14.7 μm for stents made of SA, SA-CYS and SA-CYS-NF, respectively.
33970399	6	44	theme	strut	979:983	arg1	338.7 ± 29.3 μm					1001:1015	338.7 ± 29.3 μm	1001:1015	338.7 ± 29.3 μm	1001:1015	The thickness of the strut was found to be 338.7 ± 29.3 μm, 262.5 ± 14.7 μm and 237.1 ± 14.7 μm for stents made of SA, SA-CYS and SA-CYS-NF, respectively.
33970399	10	45	theme	cell	1536:1539	arg1	studies					1549:1555	In vitro cell culture studies	1527:1555	In vitro cell culture studies	1527:1555	In vitro cell culture studies revealed that stents had no cytotoxic effects on human umbilical vein endothelial cells (HUVECs) and Raw 264.7 cells (i.e., Monocyte/macrophage-like cells), supporting that stents are biocompatible and can be explored for future clinical applications.
33970399	3	46	theme	cysteine-sodium	444:458	arg1	resources					397:405	hydrogel (~5 ml) resources	380:405	hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe	380:621	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	46	theme	cysteine-sodium	444:458	arg1	SA-CYS					470:475	SA-CYS	470:475	SA-CYS	470:475	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	46	theme	cysteine-sodium	444:458	arg1	alginate					460:467	10%w/v cysteine-sodium alginate	437:467	10%w/v cysteine-sodium alginate (SA-CYS)	437:476	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	5	47	with	BDS	861:863	arg1	viscosity					881:889	sufficient viscosity	870:889	sufficient viscosity	870:889	RESULTS It was demonstrated that alginate composition (10%w/v) offered BDS with sufficient viscosity that defined the thickness and swelling ratio of the stent struts.
33970399	3	48	theme	%	439:439	arg1	resources					397:405	hydrogel (~5 ml) resources	380:405	hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe	380:621	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	48	theme	%	439:439	arg1	SA-CYS					470:475	SA-CYS	470:475	SA-CYS	470:475	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	48	theme	%	439:439	arg1	alginate					460:467	10%w/v cysteine-sodium alginate	437:467	10%w/v cysteine-sodium alginate (SA-CYS)	437:476	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	4	49	theme	printing	754:761	arg1	speed					763:767	printing speed	754:767	printing speed	754:767	The printability of hydrogels into 3D structures was examined based on such variables as hydrogel's viscosity, printing distance, printing speed and the nozzle size.
33970399	3	50	attach	attached	596:603	arg1	BD-syringe					612:621	the BD-syringe	608:621	the BD-syringe	608:621	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	50	attach	attached	596:603	arg2	nozzle					589:594	an 22G print head nozzle	571:594	an 22G print head nozzle attached to the BD-syringe	571:621	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	10	51	theme	vein	1622:1625	arg1	HUVECs					1646:1651	HUVECs	1646:1651	HUVECs	1646:1651	In vitro cell culture studies revealed that stents had no cytotoxic effects on human umbilical vein endothelial cells (HUVECs) and Raw 264.7 cells (i.e., Monocyte/macrophage-like cells), supporting that stents are biocompatible and can be explored for future clinical applications.
33970399	10	51	theme	vein	1622:1625	arg1	cells					1639:1643	human umbilical vein endothelial cells	1606:1643	human umbilical vein endothelial cells (HUVECs)	1606:1652	In vitro cell culture studies revealed that stents had no cytotoxic effects on human umbilical vein endothelial cells (HUVECs) and Raw 264.7 cells (i.e., Monocyte/macrophage-like cells), supporting that stents are biocompatible and can be explored for future clinical applications.
33970399	10	52	theme	future	1779:1784	arg1	applications					1795:1806	future clinical applications	1779:1806	future clinical applications	1779:1806	In vitro cell culture studies revealed that stents had no cytotoxic effects on human umbilical vein endothelial cells (HUVECs) and Raw 264.7 cells (i.e., Monocyte/macrophage-like cells), supporting that stents are biocompatible and can be explored for future clinical applications.
33970399	3	53	theme	%	485:485	arg1	resources					397:405	hydrogel (~5 ml) resources	380:405	hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe	380:621	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	53	theme	%	485:485	arg1	alginate					506:513	10%w/v cysteine-sodium alginate	483:513	10%w/v cysteine-sodium alginate	483:513	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	1	54	theme	heart	180:184	arg1	disease					186:192	coronary heart disease	171:192	coronary heart disease	171:192	PURPOSE This study aimed to develop personalized biodegradable stent (BDS) for the treatment of coronary heart disease.
33970399	3	55	with	alginate	422:429	arg1	SA-CYS-NF					544:552	SA-CYS-NF	544:552	SA-CYS-NF	544:552	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	55	with	alginate	422:429	arg1	PLA-nanofibers					528:541	0.4%w/v PLA-nanofibers	520:541	0.4%w/v PLA-nanofibers (SA-CYS-NF)	520:553	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	5	56	theme	stent	944:948	arg1	struts					950:955	the stent struts	940:955	the stent struts	940:955	RESULTS It was demonstrated that alginate composition (10%w/v) offered BDS with sufficient viscosity that defined the thickness and swelling ratio of the stent struts.
33970399	8	57	theme	printed	1303:1309	arg1	stable					1362:1367	stable	1362:1367	stable	1362:1367	CONCLUSION The printed stents had sufficient mechanical strength and were stable against pseudo-physiological wall shear stress.
33970399	8	57	theme	printed	1303:1309	arg1	stents					1311:1316	The printed stents	1299:1316	The printed stents	1299:1316	CONCLUSION The printed stents had sufficient mechanical strength and were stable against pseudo-physiological wall shear stress.
33970399	3	58	theme	cysteine-sodium	490:504	arg1	resources					397:405	hydrogel (~5 ml) resources	380:405	hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe	380:621	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	58	theme	cysteine-sodium	490:504	arg1	alginate					506:513	10%w/v cysteine-sodium alginate	483:513	10%w/v cysteine-sodium alginate	483:513	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	1	59	theme	biodegradable	124:136	arg1	BDS					145:147	BDS	145:147	BDS	145:147	PURPOSE This study aimed to develop personalized biodegradable stent (BDS) for the treatment of coronary heart disease.
33970399	1	59	theme	biodegradable	124:136	arg1	stent					138:142	personalized biodegradable stent	111:142	personalized biodegradable stent (BDS) for the treatment of coronary heart disease	111:192	PURPOSE This study aimed to develop personalized biodegradable stent (BDS) for the treatment of coronary heart disease.
33970399	4	60	theme	nozzle	777:782	arg1	size					784:787	the nozzle size	773:787	the nozzle size	773:787	The printability of hydrogels into 3D structures was examined based on such variables as hydrogel's viscosity, printing distance, printing speed and the nozzle size.
33970399	5	61	theme	swelling	922:929	arg1	ratio					931:935	the thickness and swelling ratio	904:935	the thickness and swelling ratio of the stent struts	904:955	RESULTS It was demonstrated that alginate composition (10%w/v) offered BDS with sufficient viscosity that defined the thickness and swelling ratio of the stent struts.
33970399	2	62	theme	fast	258:261	arg1	fabrication					263:273	easy and fast fabrication	249:273	easy and fast fabrication of BDS with enhanced reproducibility and efficacy	249:323	Three-dimensional (3D) printing technique has offered easy and fast fabrication of BDS with enhanced reproducibility and efficacy.
33970399	8	63	theme	mechanical	1333:1342	arg1	strength					1344:1351	sufficient mechanical strength	1322:1351	sufficient mechanical strength	1322:1351	CONCLUSION The printed stents had sufficient mechanical strength and were stable against pseudo-physiological wall shear stress.
33970399	1	64	dep	PURPOSE	75:81	arg1	aimed					94:98	aimed	94:98	aimed to develop personalized biodegradable stent (BDS) for the treatment of coronary heart disease	94:192	PURPOSE This study aimed to develop personalized biodegradable stent (BDS) for the treatment of coronary heart disease.
33970399	3	65	theme	%	523:523	arg1	SA-CYS-NF					544:552	SA-CYS-NF	544:552	SA-CYS-NF	544:552	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	65	theme	%	523:523	arg1	PLA-nanofibers					528:541	0.4%w/v PLA-nanofibers	520:541	0.4%w/v PLA-nanofibers (SA-CYS-NF)	520:553	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	5	66	theme	%	847:847	arg1	w/v					848:850	10%w/v	845:850	10%w/v	845:850	RESULTS It was demonstrated that alginate composition (10%w/v) offered BDS with sufficient viscosity that defined the thickness and swelling ratio of the stent struts.
33970399	5	66	theme	%	847:847	arg1	composition					832:842	alginate composition	823:842	alginate composition (10%w/v)	823:851	RESULTS It was demonstrated that alginate composition (10%w/v) offered BDS with sufficient viscosity that defined the thickness and swelling ratio of the stent struts.
33970399	3	67	theme	10	483:484	arg1	%					485:485	%	485:485	%	485:485	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	8	68	theme	pseudo-physiological	1377:1396	arg1	stress					1409:1414	pseudo-physiological wall shear stress	1377:1414	pseudo-physiological wall shear stress	1377:1414	CONCLUSION The printed stents had sufficient mechanical strength and were stable against pseudo-physiological wall shear stress.
33970399	4	69	theme	3D	659:660	arg1	structures					662:671	3D structures	659:671	3D structures	659:671	The printability of hydrogels into 3D structures was examined based on such variables as hydrogel's viscosity, printing distance, printing speed and the nozzle size.
33970399	7	70	theme	initial	1188:1194	arg1	30 min					1196:1201	the initial 30 min	1184:1201	the initial 30 min	1184:1201	SA-CYS-NF stent displayed the highest swelling ratio of 38.8 ± 2.9% at the initial 30 min, whereas stents made of SA and SA-CYS had 23.1 ± 2.4% and 22.0 ± 2.4%, respectively.
33970399	3	71	theme	BDS	347:349	arg1	BDS					347:349	BDS	347:349	BDS	347:349	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	71	theme	BDS	347:349	arg1	variety					336:342	A variety	334:342	METHODS A variety of BDS	326:349	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	8	72	theme	shear	1403:1407	arg1	stress					1409:1414	pseudo-physiological wall shear stress	1377:1414	pseudo-physiological wall shear stress	1377:1414	CONCLUSION The printed stents had sufficient mechanical strength and were stable against pseudo-physiological wall shear stress.
33970399	3	73	theme	%	410:410	arg1	SA					432:433	SA	432:433	SA	432:433	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	73	theme	%	410:410	arg1	alginate					422:429	10%w/v sodium alginate	408:429	10%w/v sodium alginate (SA)	408:434	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	73	theme	%	410:410	arg1	resources					397:405	hydrogel (~5 ml) resources	380:405	hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe	380:621	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	5	74	theme	alginate	823:830	arg1	w/v					848:850	10%w/v	845:850	10%w/v	845:850	RESULTS It was demonstrated that alginate composition (10%w/v) offered BDS with sufficient viscosity that defined the thickness and swelling ratio of the stent struts.
33970399	5	74	theme	alginate	823:830	arg1	composition					832:842	alginate composition	823:842	alginate composition (10%w/v)	823:851	RESULTS It was demonstrated that alginate composition (10%w/v) offered BDS with sufficient viscosity that defined the thickness and swelling ratio of the stent struts.
33970399	5	75	dep	RESULTS	790:796	arg1	demonstrated					805:816	demonstrated	805:816	was demonstrated that alginate composition (10%w/v) offered BDS with sufficient viscosity that defined the thickness and swelling ratio of the stent struts	801:955	RESULTS It was demonstrated that alginate composition (10%w/v) offered BDS with sufficient viscosity that defined the thickness and swelling ratio of the stent struts.
33970399	0	76	theme	3D	27:28	arg1	Stent					68:72	3D Printed Hydrogel-Based Cardiovascular Stent	27:72	3D Printed Hydrogel-Based Cardiovascular Stent	27:72	Parametric Optimization of 3D Printed Hydrogel-Based Cardiovascular Stent.
33970399	7	77	contain	had	1241:1243	arg1	stents					1212:1217	stents	1212:1217	stents made of SA and SA-CYS	1212:1239	SA-CYS-NF stent displayed the highest swelling ratio of 38.8 ± 2.9% at the initial 30 min, whereas stents made of SA and SA-CYS had 23.1 ± 2.4% and 22.0 ± 2.4%, respectively.
33970399	7	77	contain	had	1241:1243	arg2	%					1271:1271	22.0 ± 2.4%	1261:1271	22.0 ± 2.4%	1261:1271	SA-CYS-NF stent displayed the highest swelling ratio of 38.8 ± 2.9% at the initial 30 min, whereas stents made of SA and SA-CYS had 23.1 ± 2.4% and 22.0 ± 2.4%, respectively.
33970399	7	77	contain	had	1241:1243	arg2	%					1255:1255	23.1 ± 2.4%	1245:1255	23.1 ± 2.4%	1245:1255	SA-CYS-NF stent displayed the highest swelling ratio of 38.8 ± 2.9% at the initial 30 min, whereas stents made of SA and SA-CYS had 23.1 ± 2.4% and 22.0 ± 2.4%, respectively.
33970399	3	78	dep	METHODS	326:332	arg1	BDS					347:349	BDS	347:349	BDS	347:349	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	78	dep	METHODS	326:332	arg1	variety					336:342	A variety	334:342	METHODS A variety of BDS	326:349	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	4	79	theme	hydrogels	644:652	arg1	printability					628:639	The printability	624:639	The printability of hydrogels into 3D structures	624:671	The printability of hydrogels into 3D structures was examined based on such variables as hydrogel's viscosity, printing distance, printing speed and the nozzle size.
33970399	10	80	theme	human	1606:1610	arg1	HUVECs					1646:1651	HUVECs	1646:1651	HUVECs	1646:1651	In vitro cell culture studies revealed that stents had no cytotoxic effects on human umbilical vein endothelial cells (HUVECs) and Raw 264.7 cells (i.e., Monocyte/macrophage-like cells), supporting that stents are biocompatible and can be explored for future clinical applications.
33970399	10	80	theme	human	1606:1610	arg1	cells					1639:1643	human umbilical vein endothelial cells	1606:1643	human umbilical vein endothelial cells (HUVECs)	1606:1652	In vitro cell culture studies revealed that stents had no cytotoxic effects on human umbilical vein endothelial cells (HUVECs) and Raw 264.7 cells (i.e., Monocyte/macrophage-like cells), supporting that stents are biocompatible and can be explored for future clinical applications.
33970399	0	81	theme	Hydrogel-Based	38:51	arg1	Stent					68:72	3D Printed Hydrogel-Based Cardiovascular Stent	27:72	3D Printed Hydrogel-Based Cardiovascular Stent	27:72	Parametric Optimization of 3D Printed Hydrogel-Based Cardiovascular Stent.
33970399	3	82	dep	resources	397:405	arg1	resources					397:405	hydrogel (~5 ml) resources	380:405	hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe	380:621	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	82	dep	resources	397:405	arg1	alginate					460:467	10%w/v cysteine-sodium alginate	437:467	10%w/v cysteine-sodium alginate (SA-CYS)	437:476	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	82	dep	resources	397:405	arg1	SA					432:433	SA	432:433	SA	432:433	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	82	dep	resources	397:405	arg1	SA-CYS					470:475	SA-CYS	470:475	SA-CYS	470:475	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	82	dep	resources	397:405	arg1	alginate					422:429	10%w/v sodium alginate	408:429	10%w/v sodium alginate (SA)	408:434	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	82	dep	resources	397:405	arg1	alginate					506:513	10%w/v cysteine-sodium alginate	483:513	10%w/v cysteine-sodium alginate	483:513	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	83	theme	sodium	415:420	arg1	SA					432:433	SA	432:433	SA	432:433	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	83	theme	sodium	415:420	arg1	alginate					422:429	10%w/v sodium alginate	408:429	10%w/v sodium alginate (SA)	408:434	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	83	theme	sodium	415:420	arg1	resources					397:405	hydrogel (~5 ml) resources	380:405	hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe	380:621	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	0	84	theme	Stent	68:72	arg1	Optimization					11:22	Parametric Optimization	0:22	Parametric Optimization of 3D Printed Hydrogel-Based Cardiovascular Stent.	0:73	Parametric Optimization of 3D Printed Hydrogel-Based Cardiovascular Stent.
33970399	7	85	theme	highest	1143:1149	arg1	ratio					1160:1164	the highest swelling ratio	1139:1164	the highest swelling ratio of 38.8 ± 2.9%	1139:1179	SA-CYS-NF stent displayed the highest swelling ratio of 38.8 ± 2.9% at the initial 30 min, whereas stents made of SA and SA-CYS had 23.1 ± 2.4% and 22.0 ± 2.4%, respectively.
33970399	3	86	theme	22G	574:576	arg1	nozzle					589:594	an 22G print head nozzle	571:594	an 22G print head nozzle attached to the BD-syringe	571:621	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	3	87	theme	10	437:438	arg1	%					439:439	%	439:439	%	439:439	METHODS A variety of BDS were printed with 3 types of hydrogel (~5 ml) resources (10%w/v sodium alginate (SA), 10%w/v cysteine-sodium alginate (SA-CYS), and 10%w/v cysteine-sodium alginate with 0.4%w/v PLA-nanofibers (SA-CYS-NF)) dispersed from an 22G print head nozzle attached to the BD-syringe.
33970399	10	88	dep	In	1527:1528	arg1	vitro					1530:1534	vitro	1530:1534	vitro	1530:1534	In vitro cell culture studies revealed that stents had no cytotoxic effects on human umbilical vein endothelial cells (HUVECs) and Raw 264.7 cells (i.e., Monocyte/macrophage-like cells), supporting that stents are biocompatible and can be explored for future clinical applications.
33970399	9	89	theme	biodegradation	1491:1504	arg1	rates					1506:1510	the biodegradation rates	1487:1510	the biodegradation rates of the stents	1487:1524	An addition of nanofibers to alginate hydrogel significantly enhanced the biodegradation rates of the stents.
33970399	2	90	dep	Three-dimensional	195:211	arg1	3D					214:215	3D	214:215	3D	214:215	Three-dimensional (3D) printing technique has offered easy and fast fabrication of BDS with enhanced reproducibility and efficacy.
33970399	2	91	theme	Three-dimensional	195:211	arg1	technique					227:235	Three-dimensional (3D) printing technique	195:235	Three-dimensional (3D) printing technique	195:235	Three-dimensional (3D) printing technique has offered easy and fast fabrication of BDS with enhanced reproducibility and efficacy.
33970399	5	92	theme	sufficient	870:879	arg1	viscosity					881:889	sufficient viscosity	870:889	sufficient viscosity	870:889	RESULTS It was demonstrated that alginate composition (10%w/v) offered BDS with sufficient viscosity that defined the thickness and swelling ratio of the stent struts.
33970399	7	93	theme	SA-CYS-NF	1113:1121	arg1	stent					1123:1127	SA-CYS-NF stent	1113:1127	SA-CYS-NF stent	1113:1127	SA-CYS-NF stent displayed the highest swelling ratio of 38.8 ± 2.9% at the initial 30 min, whereas stents made of SA and SA-CYS had 23.1 ± 2.4% and 22.0 ± 2.4%, respectively.
33970399	8	94	contain	had	1318:1320	arg2	strength					1344:1351	sufficient mechanical strength	1322:1351	sufficient mechanical strength	1322:1351	CONCLUSION The printed stents had sufficient mechanical strength and were stable against pseudo-physiological wall shear stress.
33970399	8	94	contain	had	1318:1320	arg1	stable					1362:1367	stable	1362:1367	stable	1362:1367	CONCLUSION The printed stents had sufficient mechanical strength and were stable against pseudo-physiological wall shear stress.
33970399	8	94	contain	had	1318:1320	arg1	stents					1311:1316	The printed stents	1299:1316	The printed stents	1299:1316	CONCLUSION The printed stents had sufficient mechanical strength and were stable against pseudo-physiological wall shear stress.
33970399	4	95	theme	such	695:698	arg1	variables					700:708	such variables	695:708	such variables as hydrogel's viscosity, printing distance, printing speed and the nozzle size	695:787	The printability of hydrogels into 3D structures was examined based on such variables as hydrogel's viscosity, printing distance, printing speed and the nozzle size.
32614762	10	0	dep	Strains	1119:1125	arg1	Strains					1119:1125	Strains	1119:1125	Strains Hs20B0-1T and Hs30E4-3T	1119:1149	Strains Hs20B0-1T and Hs30E4-3T had 75.4 % ANI similarity and 21.1 % DDH similarity to each other.
32614762	10	0	dep	Strains	1119:1125	arg1	Hs30E4-3T					1141:1149	Hs30E4-3T	1141:1149	Hs30E4-3T	1141:1149	Strains Hs20B0-1T and Hs30E4-3T had 75.4 % ANI similarity and 21.1 % DDH similarity to each other.
32614762	10	0	dep	Strains	1119:1125	arg1	Hs20B0-1T					1127:1135	Hs20B0-1T	1127:1135	Hs20B0-1T	1127:1135	Strains Hs20B0-1T and Hs30E4-3T had 75.4 % ANI similarity and 21.1 % DDH similarity to each other.
32614762	10	1	contain	had	1151:1153	arg2	similarity					1192:1201	21.1 % DDH similarity	1181:1201	21.1 % DDH similarity	1181:1201	Strains Hs20B0-1T and Hs30E4-3T had 75.4 % ANI similarity and 21.1 % DDH similarity to each other.
32614762	10	1	contain	had	1151:1153	arg2	similarity					1166:1175	75.4 % ANI similarity	1155:1175	75.4 % ANI similarity	1155:1175	Strains Hs20B0-1T and Hs30E4-3T had 75.4 % ANI similarity and 21.1 % DDH similarity to each other.
32614762	10	1	contain	had	1151:1153	arg1	Strains					1119:1125	Strains	1119:1125	Strains Hs20B0-1T and Hs30E4-3T	1119:1149	Strains Hs20B0-1T and Hs30E4-3T had 75.4 % ANI similarity and 21.1 % DDH similarity to each other.
32614762	10	1	contain	had	1151:1153	arg1	Hs30E4-3T					1141:1149	Hs30E4-3T	1141:1149	Hs30E4-3T	1141:1149	Strains Hs20B0-1T and Hs30E4-3T had 75.4 % ANI similarity and 21.1 % DDH similarity to each other.
32614762	10	1	contain	had	1151:1153	arg1	Hs20B0-1T					1127:1135	Hs20B0-1T	1127:1135	Hs20B0-1T	1127:1135	Strains Hs20B0-1T and Hs30E4-3T had 75.4 % ANI similarity and 21.1 % DDH similarity to each other.
32614762	7	2	theme	gene	711:714	arg1	similarity					725:734	The 16S rRNA gene sequence similarity	698:734	The 16S rRNA gene sequence similarity between strains Hs20B0-1T and Hs30E4-3T	698:774	The 16S rRNA gene sequence similarity between strains Hs20B0-1T and Hs30E4-3T was 95.7 %.
32614762	7	2	theme	gene	711:714	arg1	%					785:785	95.7 %	780:785	95.7 %	780:785	The 16S rRNA gene sequence similarity between strains Hs20B0-1T and Hs30E4-3T was 95.7 %.
32614762	13	3	dep	Lactococcus	1641:1651	arg1	hodotermopsidis					1653:1667	hodotermopsidis	1653:1667	hodotermopsidis	1653:1667	In conclusion, two novel species of the genus Lactococcus are proposed, namely Lactococcus insecticola Hs20B0-1T (=JCM 33485T=DSM 110147T) and Lactococcus hodotermopsidis Hs30E4-3T (=JCM 33486T=DSM 110148T), respectively.
32614762	2	4	theme	Hodotermopsis	131:143	arg1	sjostedti					145:153	the wood-feeding lower termite Hodotermopsis sjostedti	100:153	the wood-feeding lower termite Hodotermopsis sjostedti	100:153	nov., isolated from the gut of the wood-feeding lower termite Hodotermopsis sjostedti.
32614762	5	5	theme	gene	503:506	arg1	sequences					508:516	16S rRNA gene and rpoB gene sequences	480:516	sequences	508:516	Strain Hs20B0-1T was related to Lactococcus piscium DSM 6634T showing 96.3 and 84.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively.
32614762	12	6	from	species	1453:1459	arg1	distinguishable					1387:1401	distinguishable	1387:1401	distinguishable	1387:1401	The two strains, Hs20B0-1T and Hs30E4-3T, are distinguishable from each other and other established Lactococcus species phylogenetically and phenotypically.
32614762	12	6	from	species	1453:1459	arg1	strains					1349:1355	The two strains	1341:1355	The two strains	1341:1355	The two strains, Hs20B0-1T and Hs30E4-3T, are distinguishable from each other and other established Lactococcus species phylogenetically and phenotypically.
32614762	13	7	theme	Lactococcus	1544:1554	arg1	species					1523:1529	two novel species	1513:1529	two novel species of the genus Lactococcus	1513:1554	In conclusion, two novel species of the genus Lactococcus are proposed, namely Lactococcus insecticola Hs20B0-1T (=JCM 33485T=DSM 110147T) and Lactococcus hodotermopsidis Hs30E4-3T (=JCM 33486T=DSM 110148T), respectively.
32614762	5	8	theme	rRNA	484:487	arg1	gene					489:492	16S rRNA gene and rpoB gene sequences	480:516	gene	489:492	Strain Hs20B0-1T was related to Lactococcus piscium DSM 6634T showing 96.3 and 84.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively.
32614762	6	9	theme	DSM	587:589	arg1	20686T					591:596	Lactococcus plantarum DSM 20686T	565:596	Lactococcus plantarum DSM 20686T showing 94.8 and 82.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively	565:695	Strain Hs30E4-3T was related to Lactococcus plantarum DSM 20686T showing 94.8 and 82.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively.
32614762	3	10	theme	sjostedti	295:303	arg1	gut					252:254	the gut	248:254	the gut of the damp-wood termite Hodotermopsis sjostedti	248:303	Two strains of lactic acid bacteria, designated Hs20B0-1T and Hs30E4-3T, were isolated from the gut of the damp-wood termite Hodotermopsis sjostedti.
32614762	6	11	dep	%	620:620	arg1	94.8					606:609	94.8	606:609	94.8	606:609	Strain Hs30E4-3T was related to Lactococcus plantarum DSM 20686T showing 94.8 and 82.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively.
32614762	6	11	dep	%	620:620	arg1	82.2					615:618	82.2	615:618	82.2	615:618	Strain Hs30E4-3T was related to Lactococcus plantarum DSM 20686T showing 94.8 and 82.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively.
32614762	6	12	theme	Lactococcus	565:575	arg1	20686T					591:596	Lactococcus plantarum DSM 20686T	565:596	Lactococcus plantarum DSM 20686T showing 94.8 and 82.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively	565:695	Strain Hs30E4-3T was related to Lactococcus plantarum DSM 20686T showing 94.8 and 82.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively.
32614762	3	13	theme	termite	273:279	arg1	sjostedti					295:303	the damp-wood termite Hodotermopsis sjostedti	259:303	the damp-wood termite Hodotermopsis sjostedti	259:303	Two strains of lactic acid bacteria, designated Hs20B0-1T and Hs30E4-3T, were isolated from the gut of the damp-wood termite Hodotermopsis sjostedti.
32614762	6	14	dep	similarity	631:640	arg1	%					620:620	%	620:620	%	620:620	Strain Hs30E4-3T was related to Lactococcus plantarum DSM 20686T showing 94.8 and 82.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively.
32614762	1	15	dep	sp	65:66	arg1	hodotermopsidis					49:63	nov. and Lactococcus hodotermopsidis	28:63	nov. and Lactococcus hodotermopsidis	28:63	nov. and Lactococcus hodotermopsidis sp.
32614762	10	16	theme	DDH	1188:1190	arg1	similarity					1192:1201	21.1 % DDH similarity	1181:1201	21.1 % DDH similarity	1181:1201	Strains Hs20B0-1T and Hs30E4-3T had 75.4 % ANI similarity and 21.1 % DDH similarity to each other.
32614762	8	17	theme	average	836:842	arg1	identity					855:862	pairwise average nucleotide identity	827:862	pairwise average nucleotide identity (ANI)	827:868	Furthermore, genomic comparisons using pairwise average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) analyses between strain Hs20B0-1T and L. piscium DSM 6634T resulted in values of 73.5 and 20.1 %, respectively.
32614762	8	17	theme	average	836:842	arg1	ANI					865:867	ANI	865:867	ANI	865:867	Furthermore, genomic comparisons using pairwise average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) analyses between strain Hs20B0-1T and L. piscium DSM 6634T resulted in values of 73.5 and 20.1 %, respectively.
32614762	5	18	theme	Lactococcus	403:413	arg1	6634T					427:431	Lactococcus piscium DSM 6634T	403:431	Lactococcus piscium DSM 6634T showing 96.3 and 84.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively	403:530	Strain Hs20B0-1T was related to Lactococcus piscium DSM 6634T showing 96.3 and 84.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively.
32614762	10	19	theme	21.1	1181:1184	arg1	%					1186:1186	%	1186:1186	%	1186:1186	Strains Hs20B0-1T and Hs30E4-3T had 75.4 % ANI similarity and 21.1 % DDH similarity to each other.
32614762	10	20	theme	ANI	1162:1164	arg1	similarity					1166:1175	75.4 % ANI similarity	1155:1175	75.4 % ANI similarity	1155:1175	Strains Hs20B0-1T and Hs30E4-3T had 75.4 % ANI similarity and 21.1 % DDH similarity to each other.
32614762	5	21	theme	DSM	423:425	arg1	6634T					427:431	Lactococcus piscium DSM 6634T	403:431	Lactococcus piscium DSM 6634T showing 96.3 and 84.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively	403:530	Strain Hs20B0-1T was related to Lactococcus piscium DSM 6634T showing 96.3 and 84.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively.
32614762	11	22	theme	peptidoglycan	1232:1244	arg1	types					1246:1250	The cell-wall peptidoglycan types	1218:1250	The cell-wall peptidoglycan types of strains Hs20B0-1T and Hs30E4-3T	1218:1285	The cell-wall peptidoglycan types of strains Hs20B0-1T and Hs30E4-3T were A4α, Lys-Asp and A3α, Lys-Thr-Ala, respectively.
32614762	11	22	theme	peptidoglycan	1232:1244	arg1	Lys-Asp					1297:1303	Lys-Asp	1297:1303	Lys-Asp	1297:1303	The cell-wall peptidoglycan types of strains Hs20B0-1T and Hs30E4-3T were A4α, Lys-Asp and A3α, Lys-Thr-Ala, respectively.
32614762	8	23	theme	%	1005:1005	arg1	values					981:986	values	981:986	values	981:986	Furthermore, genomic comparisons using pairwise average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) analyses between strain Hs20B0-1T and L. piscium DSM 6634T resulted in values of 73.5 and 20.1 %, respectively.
32614762	13	24	theme	33486T=DSM	1685:1694	arg1	Hs30E4-3T					1669:1677	Lactococcus hodotermopsidis Hs30E4-3T	1641:1677	Lactococcus hodotermopsidis Hs30E4-3T (=JCM 33486T=DSM 110148T)	1641:1703	In conclusion, two novel species of the genus Lactococcus are proposed, namely Lactococcus insecticola Hs20B0-1T (=JCM 33485T=DSM 110147T) and Lactococcus hodotermopsidis Hs30E4-3T (=JCM 33486T=DSM 110148T), respectively.
32614762	13	24	theme	33486T=DSM	1685:1694	arg1	110148T					1696:1702	=JCM 33486T=DSM 110148T	1680:1702	=JCM 33486T=DSM 110148T	1680:1702	In conclusion, two novel species of the genus Lactococcus are proposed, namely Lactococcus insecticola Hs20B0-1T (=JCM 33485T=DSM 110147T) and Lactococcus hodotermopsidis Hs30E4-3T (=JCM 33486T=DSM 110148T), respectively.
32614762	3	25	theme	lactic	171:176	arg1	bacteria					183:190	lactic acid bacteria	171:190	lactic acid bacteria	171:190	Two strains of lactic acid bacteria, designated Hs20B0-1T and Hs30E4-3T, were isolated from the gut of the damp-wood termite Hodotermopsis sjostedti.
32614762	9	26	theme	72.8	1043:1046	arg1	%					1048:1048	%	1048:1048	%	1048:1048	Strain Hs30E4-3T had 72.8 % ANI similarity and 21.3 % DDH similarity to L. plantarum DSM 20686T.
32614762	8	27	theme	genomic	801:807	arg1	comparisons					809:819	genomic comparisons	801:819	genomic comparisons using pairwise average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) analyses between strain Hs20B0-1T and L. piscium DSM 6634T	801:967	Furthermore, genomic comparisons using pairwise average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) analyses between strain Hs20B0-1T and L. piscium DSM 6634T resulted in values of 73.5 and 20.1 %, respectively.
32614762	9	28	theme	DDH	1076:1078	arg1	similarity					1080:1089	21.3 % DDH similarity	1069:1089	21.3 % DDH similarity	1069:1089	Strain Hs30E4-3T had 72.8 % ANI similarity and 21.3 % DDH similarity to L. plantarum DSM 20686T.
32614762	5	29	dep	%	455:455	arg1	96.3					441:444	96.3	441:444	96.3	441:444	Strain Hs20B0-1T was related to Lactococcus piscium DSM 6634T showing 96.3 and 84.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively.
32614762	5	29	dep	%	455:455	arg1	84.2					450:453	84.2	450:453	84.2	450:453	Strain Hs20B0-1T was related to Lactococcus piscium DSM 6634T showing 96.3 and 84.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively.
32614762	9	30	theme	ANI	1050:1052	arg1	similarity					1054:1063	72.8 % ANI similarity	1043:1063	72.8 % ANI similarity	1043:1063	Strain Hs30E4-3T had 72.8 % ANI similarity and 21.3 % DDH similarity to L. plantarum DSM 20686T.
32614762	10	31	theme	%	1160:1160	arg1	similarity					1166:1175	75.4 % ANI similarity	1155:1175	75.4 % ANI similarity	1155:1175	Strains Hs20B0-1T and Hs30E4-3T had 75.4 % ANI similarity and 21.1 % DDH similarity to each other.
32614762	8	32	theme	DNA-DNA	882:888	arg1	DDH					905:907	DDH	905:907	DDH	905:907	Furthermore, genomic comparisons using pairwise average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) analyses between strain Hs20B0-1T and L. piscium DSM 6634T resulted in values of 73.5 and 20.1 %, respectively.
32614762	8	32	theme	DNA-DNA	882:888	arg1	hybridization					890:902	digital DNA-DNA hybridization	874:902	digital DNA-DNA hybridization (DDH) analyses between strain Hs20B0-1T and L. piscium DSM 6634T	874:967	Furthermore, genomic comparisons using pairwise average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) analyses between strain Hs20B0-1T and L. piscium DSM 6634T resulted in values of 73.5 and 20.1 %, respectively.
32614762	0	33	theme	Lactococcus	0:10	arg1	sp					24:25	Lactococcus insecticola sp	0:25	Lactococcus insecticola sp.	0:26	Lactococcus insecticola sp.
32614762	5	34	from	similarity	466:475	arg1	gene					489:492	16S rRNA gene and rpoB gene sequences	480:516	gene	489:492	Strain Hs20B0-1T was related to Lactococcus piscium DSM 6634T showing 96.3 and 84.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively.
32614762	5	34	from	similarity	466:475	arg1	sequences					508:516	16S rRNA gene and rpoB gene sequences	480:516	sequences	508:516	Strain Hs20B0-1T was related to Lactococcus piscium DSM 6634T showing 96.3 and 84.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively.
32614762	12	35	theme	Lactococcus	1441:1451	arg1	species					1453:1459	each other and other established Lactococcus species	1408:1459	species	1453:1459	The two strains, Hs20B0-1T and Hs30E4-3T, are distinguishable from each other and other established Lactococcus species phylogenetically and phenotypically.
32614762	13	36	theme	=JCM	1612:1615	arg1	Hs20B0-1T					1601:1609	Lactococcus insecticola Hs20B0-1T	1577:1609	Lactococcus insecticola Hs20B0-1T (=JCM 33485T=DSM 110147T)	1577:1635	In conclusion, two novel species of the genus Lactococcus are proposed, namely Lactococcus insecticola Hs20B0-1T (=JCM 33485T=DSM 110147T) and Lactococcus hodotermopsidis Hs30E4-3T (=JCM 33486T=DSM 110148T), respectively.
32614762	13	36	theme	=JCM	1612:1615	arg1	110147T					1628:1634	=JCM 33485T=DSM 110147T	1612:1634	=JCM 33485T=DSM 110147T	1612:1634	In conclusion, two novel species of the genus Lactococcus are proposed, namely Lactococcus insecticola Hs20B0-1T (=JCM 33485T=DSM 110147T) and Lactococcus hodotermopsidis Hs30E4-3T (=JCM 33486T=DSM 110148T), respectively.
32614762	3	37	theme	bacteria	183:190	arg1	strains					160:166	Two strains	156:166	Two strains	156:166	Two strains of lactic acid bacteria, designated Hs20B0-1T and Hs30E4-3T, were isolated from the gut of the damp-wood termite Hodotermopsis sjostedti.
32614762	9	38	theme	%	1074:1074	arg1	similarity					1080:1089	21.3 % DDH similarity	1069:1089	21.3 % DDH similarity	1069:1089	Strain Hs30E4-3T had 72.8 % ANI similarity and 21.3 % DDH similarity to L. plantarum DSM 20686T.
32614762	6	39	theme	rpoB	663:666	arg1	sequences					673:681	16S rRNA gene and rpoB gene sequences	645:681	sequences	673:681	Strain Hs30E4-3T was related to Lactococcus plantarum DSM 20686T showing 94.8 and 82.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively.
32614762	13	40	theme	Lactococcus	1577:1587	arg1	Hs20B0-1T					1601:1609	Lactococcus insecticola Hs20B0-1T	1577:1609	Lactococcus insecticola Hs20B0-1T (=JCM 33485T=DSM 110147T)	1577:1635	In conclusion, two novel species of the genus Lactococcus are proposed, namely Lactococcus insecticola Hs20B0-1T (=JCM 33485T=DSM 110147T) and Lactococcus hodotermopsidis Hs30E4-3T (=JCM 33486T=DSM 110148T), respectively.
32614762	13	40	theme	Lactococcus	1577:1587	arg1	110147T					1628:1634	=JCM 33485T=DSM 110147T	1612:1634	=JCM 33485T=DSM 110147T	1612:1634	In conclusion, two novel species of the genus Lactococcus are proposed, namely Lactococcus insecticola Hs20B0-1T (=JCM 33485T=DSM 110147T) and Lactococcus hodotermopsidis Hs30E4-3T (=JCM 33486T=DSM 110148T), respectively.
32614762	5	41	dep	Lactococcus	403:413	arg1	piscium					415:421	piscium	415:421	piscium	415:421	Strain Hs20B0-1T was related to Lactococcus piscium DSM 6634T showing 96.3 and 84.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively.
32614762	9	42	theme	L.	1094:1095	arg1	20686T					1111:1116	L. plantarum DSM 20686T	1094:1116	L. plantarum DSM 20686T	1094:1116	Strain Hs30E4-3T had 72.8 % ANI similarity and 21.3 % DDH similarity to L. plantarum DSM 20686T.
32614762	6	43	theme	rRNA	649:652	arg1	gene					654:657	16S rRNA gene and rpoB gene sequences	645:681	gene	654:657	Strain Hs30E4-3T was related to Lactococcus plantarum DSM 20686T showing 94.8 and 82.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively.
32614762	6	44	from	similarity	631:640	arg1	sequences					673:681	16S rRNA gene and rpoB gene sequences	645:681	sequences	673:681	Strain Hs30E4-3T was related to Lactococcus plantarum DSM 20686T showing 94.8 and 82.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively.
32614762	6	44	from	similarity	631:640	arg1	gene					654:657	16S rRNA gene and rpoB gene sequences	645:681	gene	654:657	Strain Hs30E4-3T was related to Lactococcus plantarum DSM 20686T showing 94.8 and 82.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively.
32614762	9	45	theme	DSM	1107:1109	arg1	20686T					1111:1116	L. plantarum DSM 20686T	1094:1116	L. plantarum DSM 20686T	1094:1116	Strain Hs30E4-3T had 72.8 % ANI similarity and 21.3 % DDH similarity to L. plantarum DSM 20686T.
32614762	10	46	theme	75.4	1155:1158	arg1	%					1160:1160	%	1160:1160	%	1160:1160	Strains Hs20B0-1T and Hs30E4-3T had 75.4 % ANI similarity and 21.1 % DDH similarity to each other.
32614762	12	47	theme	established	1429:1439	arg1	species					1453:1459	each other and other established Lactococcus species	1408:1459	species	1453:1459	The two strains, Hs20B0-1T and Hs30E4-3T, are distinguishable from each other and other established Lactococcus species phylogenetically and phenotypically.
32614762	8	48	theme	L.	948:949	arg1	6634T					963:967	L. piscium DSM 6634T	948:967	L. piscium DSM 6634T	948:967	Furthermore, genomic comparisons using pairwise average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) analyses between strain Hs20B0-1T and L. piscium DSM 6634T resulted in values of 73.5 and 20.1 %, respectively.
32614762	8	49	theme	DSM	959:961	arg1	6634T					963:967	L. piscium DSM 6634T	948:967	L. piscium DSM 6634T	948:967	Furthermore, genomic comparisons using pairwise average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) analyses between strain Hs20B0-1T and L. piscium DSM 6634T resulted in values of 73.5 and 20.1 %, respectively.
32614762	2	50	theme	wood-feeding	104:115	arg1	sjostedti					145:153	the wood-feeding lower termite Hodotermopsis sjostedti	100:153	the wood-feeding lower termite Hodotermopsis sjostedti	100:153	nov., isolated from the gut of the wood-feeding lower termite Hodotermopsis sjostedti.
32614762	7	51	theme	sequence	716:723	arg1	similarity					725:734	The 16S rRNA gene sequence similarity	698:734	The 16S rRNA gene sequence similarity between strains Hs20B0-1T and Hs30E4-3T	698:774	The 16S rRNA gene sequence similarity between strains Hs20B0-1T and Hs30E4-3T was 95.7 %.
32614762	7	51	theme	sequence	716:723	arg1	%					785:785	95.7 %	780:785	95.7 %	780:785	The 16S rRNA gene sequence similarity between strains Hs20B0-1T and Hs30E4-3T was 95.7 %.
32614762	6	52	theme	Strain	533:538	arg1	Hs30E4-3T					540:548	Strain Hs30E4-3T	533:548	Strain Hs30E4-3T	533:548	Strain Hs30E4-3T was related to Lactococcus plantarum DSM 20686T showing 94.8 and 82.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively.
32614762	7	53	theme	rRNA	706:709	arg1	similarity					725:734	The 16S rRNA gene sequence similarity	698:734	The 16S rRNA gene sequence similarity between strains Hs20B0-1T and Hs30E4-3T	698:774	The 16S rRNA gene sequence similarity between strains Hs20B0-1T and Hs30E4-3T was 95.7 %.
32614762	7	53	theme	rRNA	706:709	arg1	%					785:785	95.7 %	780:785	95.7 %	780:785	The 16S rRNA gene sequence similarity between strains Hs20B0-1T and Hs30E4-3T was 95.7 %.
32614762	2	54	theme	sjostedti	145:153	arg1	gut					93:95	the gut	89:95	the gut of the wood-feeding lower termite Hodotermopsis sjostedti	89:153	nov., isolated from the gut of the wood-feeding lower termite Hodotermopsis sjostedti.
32614762	5	55	theme	rpoB	498:501	arg1	sequences					508:516	16S rRNA gene and rpoB gene sequences	480:516	sequences	508:516	Strain Hs20B0-1T was related to Lactococcus piscium DSM 6634T showing 96.3 and 84.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively.
32614762	2	56	theme	termite	123:129	arg1	sjostedti					145:153	the wood-feeding lower termite Hodotermopsis sjostedti	100:153	the wood-feeding lower termite Hodotermopsis sjostedti	100:153	nov., isolated from the gut of the wood-feeding lower termite Hodotermopsis sjostedti.
32614762	13	57	theme	genus	1538:1542	arg1	Lactococcus					1544:1554	the genus Lactococcus	1534:1554	the genus Lactococcus	1534:1554	In conclusion, two novel species of the genus Lactococcus are proposed, namely Lactococcus insecticola Hs20B0-1T (=JCM 33485T=DSM 110147T) and Lactococcus hodotermopsidis Hs30E4-3T (=JCM 33486T=DSM 110148T), respectively.
32614762	5	58	theme	16S	480:482	arg1	gene					489:492	16S rRNA gene and rpoB gene sequences	480:516	gene	489:492	Strain Hs20B0-1T was related to Lactococcus piscium DSM 6634T showing 96.3 and 84.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively.
32614762	6	59	dep	Lactococcus	565:575	arg1	plantarum					577:585	plantarum	577:585	plantarum	577:585	Strain Hs30E4-3T was related to Lactococcus plantarum DSM 20686T showing 94.8 and 82.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively.
32614762	7	60	theme	16S	702:704	arg1	similarity					725:734	The 16S rRNA gene sequence similarity	698:734	The 16S rRNA gene sequence similarity between strains Hs20B0-1T and Hs30E4-3T	698:774	The 16S rRNA gene sequence similarity between strains Hs20B0-1T and Hs30E4-3T was 95.7 %.
32614762	7	60	theme	16S	702:704	arg1	%					785:785	95.7 %	780:785	95.7 %	780:785	The 16S rRNA gene sequence similarity between strains Hs20B0-1T and Hs30E4-3T was 95.7 %.
32614762	13	61	theme	novel	1517:1521	arg1	species					1523:1529	two novel species	1513:1529	two novel species of the genus Lactococcus	1513:1554	In conclusion, two novel species of the genus Lactococcus are proposed, namely Lactococcus insecticola Hs20B0-1T (=JCM 33485T=DSM 110147T) and Lactococcus hodotermopsidis Hs30E4-3T (=JCM 33486T=DSM 110148T), respectively.
32614762	12	62	from	other	1413:1417	arg1	distinguishable					1387:1401	distinguishable	1387:1401	distinguishable	1387:1401	The two strains, Hs20B0-1T and Hs30E4-3T, are distinguishable from each other and other established Lactococcus species phylogenetically and phenotypically.
32614762	12	62	from	other	1413:1417	arg1	strains					1349:1355	The two strains	1341:1355	The two strains	1341:1355	The two strains, Hs20B0-1T and Hs30E4-3T, are distinguishable from each other and other established Lactococcus species phylogenetically and phenotypically.
32614762	9	63	contain	had	1039:1041	arg2	similarity					1080:1089	21.3 % DDH similarity	1069:1089	21.3 % DDH similarity	1069:1089	Strain Hs30E4-3T had 72.8 % ANI similarity and 21.3 % DDH similarity to L. plantarum DSM 20686T.
32614762	9	63	contain	had	1039:1041	arg1	Hs30E4-3T					1029:1037	Strain Hs30E4-3T	1022:1037	Strain Hs30E4-3T	1022:1037	Strain Hs30E4-3T had 72.8 % ANI similarity and 21.3 % DDH similarity to L. plantarum DSM 20686T.
32614762	9	63	contain	had	1039:1041	arg2	similarity					1054:1063	72.8 % ANI similarity	1043:1063	72.8 % ANI similarity	1043:1063	Strain Hs30E4-3T had 72.8 % ANI similarity and 21.3 % DDH similarity to L. plantarum DSM 20686T.
32614762	5	64	dep	similarity	466:475	arg1	%					455:455	%	455:455	%	455:455	Strain Hs20B0-1T was related to Lactococcus piscium DSM 6634T showing 96.3 and 84.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively.
32614762	3	65	theme	Hodotermopsis	281:293	arg1	sjostedti					295:303	the damp-wood termite Hodotermopsis sjostedti	259:303	the damp-wood termite Hodotermopsis sjostedti	259:303	Two strains of lactic acid bacteria, designated Hs20B0-1T and Hs30E4-3T, were isolated from the gut of the damp-wood termite Hodotermopsis sjostedti.
32614762	8	66	dep	L.	948:949	arg1	piscium					951:957	piscium	951:957	piscium	951:957	Furthermore, genomic comparisons using pairwise average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) analyses between strain Hs20B0-1T and L. piscium DSM 6634T resulted in values of 73.5 and 20.1 %, respectively.
32614762	8	67	theme	pairwise	827:834	arg1	identity					855:862	pairwise average nucleotide identity	827:862	pairwise average nucleotide identity (ANI)	827:868	Furthermore, genomic comparisons using pairwise average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) analyses between strain Hs20B0-1T and L. piscium DSM 6634T resulted in values of 73.5 and 20.1 %, respectively.
32614762	8	67	theme	pairwise	827:834	arg1	ANI					865:867	ANI	865:867	ANI	865:867	Furthermore, genomic comparisons using pairwise average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) analyses between strain Hs20B0-1T and L. piscium DSM 6634T resulted in values of 73.5 and 20.1 %, respectively.
32614762	6	68	theme	16S	645:647	arg1	gene					654:657	16S rRNA gene and rpoB gene sequences	645:681	gene	654:657	Strain Hs30E4-3T was related to Lactococcus plantarum DSM 20686T showing 94.8 and 82.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively.
32614762	9	69	theme	Strain	1022:1027	arg1	Hs30E4-3T					1029:1037	Strain Hs30E4-3T	1022:1037	Strain Hs30E4-3T	1022:1037	Strain Hs30E4-3T had 72.8 % ANI similarity and 21.3 % DDH similarity to L. plantarum DSM 20686T.
32614762	10	70	theme	%	1186:1186	arg1	similarity					1192:1201	21.1 % DDH similarity	1181:1201	21.1 % DDH similarity	1181:1201	Strains Hs20B0-1T and Hs30E4-3T had 75.4 % ANI similarity and 21.1 % DDH similarity to each other.
32614762	8	71	theme	nucleotide	844:853	arg1	identity					855:862	pairwise average nucleotide identity	827:862	pairwise average nucleotide identity (ANI)	827:868	Furthermore, genomic comparisons using pairwise average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) analyses between strain Hs20B0-1T and L. piscium DSM 6634T resulted in values of 73.5 and 20.1 %, respectively.
32614762	8	71	theme	nucleotide	844:853	arg1	ANI					865:867	ANI	865:867	ANI	865:867	Furthermore, genomic comparisons using pairwise average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) analyses between strain Hs20B0-1T and L. piscium DSM 6634T resulted in values of 73.5 and 20.1 %, respectively.
32614762	5	72	theme	Strain	371:376	arg1	Hs20B0-1T					378:386	Strain Hs20B0-1T	371:386	Strain Hs20B0-1T	371:386	Strain Hs20B0-1T was related to Lactococcus piscium DSM 6634T showing 96.3 and 84.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively.
32614762	3	73	theme	damp-wood	263:271	arg1	sjostedti					295:303	the damp-wood termite Hodotermopsis sjostedti	259:303	the damp-wood termite Hodotermopsis sjostedti	259:303	Two strains of lactic acid bacteria, designated Hs20B0-1T and Hs30E4-3T, were isolated from the gut of the damp-wood termite Hodotermopsis sjostedti.
32614762	13	74	theme	=JCM	1680:1683	arg1	Hs30E4-3T					1669:1677	Lactococcus hodotermopsidis Hs30E4-3T	1641:1677	Lactococcus hodotermopsidis Hs30E4-3T (=JCM 33486T=DSM 110148T)	1641:1703	In conclusion, two novel species of the genus Lactococcus are proposed, namely Lactococcus insecticola Hs20B0-1T (=JCM 33485T=DSM 110147T) and Lactococcus hodotermopsidis Hs30E4-3T (=JCM 33486T=DSM 110148T), respectively.
32614762	13	74	theme	=JCM	1680:1683	arg1	110148T					1696:1702	=JCM 33486T=DSM 110148T	1680:1702	=JCM 33486T=DSM 110148T	1680:1702	In conclusion, two novel species of the genus Lactococcus are proposed, namely Lactococcus insecticola Hs20B0-1T (=JCM 33485T=DSM 110147T) and Lactococcus hodotermopsidis Hs30E4-3T (=JCM 33486T=DSM 110148T), respectively.
32614762	6	75	theme	sequence	622:629	arg1	similarity					631:640	94.8 and 82.2 % sequence similarity	606:640	94.8 and 82.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively	606:695	Strain Hs30E4-3T was related to Lactococcus plantarum DSM 20686T showing 94.8 and 82.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively.
32614762	11	76	theme	cell-wall	1222:1230	arg1	types					1246:1250	The cell-wall peptidoglycan types	1218:1250	The cell-wall peptidoglycan types of strains Hs20B0-1T and Hs30E4-3T	1218:1285	The cell-wall peptidoglycan types of strains Hs20B0-1T and Hs30E4-3T were A4α, Lys-Asp and A3α, Lys-Thr-Ala, respectively.
32614762	11	76	theme	cell-wall	1222:1230	arg1	Lys-Asp					1297:1303	Lys-Asp	1297:1303	Lys-Asp	1297:1303	The cell-wall peptidoglycan types of strains Hs20B0-1T and Hs30E4-3T were A4α, Lys-Asp and A3α, Lys-Thr-Ala, respectively.
32614762	9	77	theme	%	1048:1048	arg1	similarity					1054:1063	72.8 % ANI similarity	1043:1063	72.8 % ANI similarity	1043:1063	Strain Hs30E4-3T had 72.8 % ANI similarity and 21.3 % DDH similarity to L. plantarum DSM 20686T.
32614762	13	78	theme	Lactococcus	1641:1651	arg1	Hs30E4-3T					1669:1677	Lactococcus hodotermopsidis Hs30E4-3T	1641:1677	Lactococcus hodotermopsidis Hs30E4-3T (=JCM 33486T=DSM 110148T)	1641:1703	In conclusion, two novel species of the genus Lactococcus are proposed, namely Lactococcus insecticola Hs20B0-1T (=JCM 33485T=DSM 110147T) and Lactococcus hodotermopsidis Hs30E4-3T (=JCM 33486T=DSM 110148T), respectively.
32614762	13	78	theme	Lactococcus	1641:1651	arg1	110148T					1696:1702	=JCM 33486T=DSM 110148T	1680:1702	=JCM 33486T=DSM 110148T	1680:1702	In conclusion, two novel species of the genus Lactococcus are proposed, namely Lactococcus insecticola Hs20B0-1T (=JCM 33485T=DSM 110147T) and Lactococcus hodotermopsidis Hs30E4-3T (=JCM 33486T=DSM 110148T), respectively.
32614762	0	79	theme	insecticola	12:22	arg1	sp					24:25	Lactococcus insecticola sp	0:25	Lactococcus insecticola sp.	0:26	Lactococcus insecticola sp.
32614762	8	80	theme	digital	874:880	arg1	DDH					905:907	DDH	905:907	DDH	905:907	Furthermore, genomic comparisons using pairwise average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) analyses between strain Hs20B0-1T and L. piscium DSM 6634T resulted in values of 73.5 and 20.1 %, respectively.
32614762	8	80	theme	digital	874:880	arg1	hybridization					890:902	digital DNA-DNA hybridization	874:902	digital DNA-DNA hybridization (DDH) analyses between strain Hs20B0-1T and L. piscium DSM 6634T	874:967	Furthermore, genomic comparisons using pairwise average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) analyses between strain Hs20B0-1T and L. piscium DSM 6634T resulted in values of 73.5 and 20.1 %, respectively.
32614762	11	81	theme	strains	1255:1261	arg1	types					1246:1250	The cell-wall peptidoglycan types	1218:1250	The cell-wall peptidoglycan types of strains Hs20B0-1T and Hs30E4-3T	1218:1285	The cell-wall peptidoglycan types of strains Hs20B0-1T and Hs30E4-3T were A4α, Lys-Asp and A3α, Lys-Thr-Ala, respectively.
32614762	11	81	theme	strains	1255:1261	arg1	Lys-Asp					1297:1303	Lys-Asp	1297:1303	Lys-Asp	1297:1303	The cell-wall peptidoglycan types of strains Hs20B0-1T and Hs30E4-3T were A4α, Lys-Asp and A3α, Lys-Thr-Ala, respectively.
32614762	3	82	theme	acid	178:181	arg1	bacteria					183:190	lactic acid bacteria	171:190	lactic acid bacteria	171:190	Two strains of lactic acid bacteria, designated Hs20B0-1T and Hs30E4-3T, were isolated from the gut of the damp-wood termite Hodotermopsis sjostedti.
32614762	9	83	theme	21.3	1069:1072	arg1	%					1074:1074	%	1074:1074	%	1074:1074	Strain Hs30E4-3T had 72.8 % ANI similarity and 21.3 % DDH similarity to L. plantarum DSM 20686T.
32614762	8	84	theme	hybridization	890:902	arg1	analyses					910:917	digital DNA-DNA hybridization (DDH) analyses	874:917	digital DNA-DNA hybridization (DDH) analyses between strain Hs20B0-1T and L. piscium DSM 6634T	874:967	Furthermore, genomic comparisons using pairwise average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) analyses between strain Hs20B0-1T and L. piscium DSM 6634T resulted in values of 73.5 and 20.1 %, respectively.
32614762	13	85	theme	33485T=DSM	1617:1626	arg1	Hs20B0-1T					1601:1609	Lactococcus insecticola Hs20B0-1T	1577:1609	Lactococcus insecticola Hs20B0-1T (=JCM 33485T=DSM 110147T)	1577:1635	In conclusion, two novel species of the genus Lactococcus are proposed, namely Lactococcus insecticola Hs20B0-1T (=JCM 33485T=DSM 110147T) and Lactococcus hodotermopsidis Hs30E4-3T (=JCM 33486T=DSM 110148T), respectively.
32614762	13	85	theme	33485T=DSM	1617:1626	arg1	110147T					1628:1634	=JCM 33485T=DSM 110147T	1612:1634	=JCM 33485T=DSM 110147T	1612:1634	In conclusion, two novel species of the genus Lactococcus are proposed, namely Lactococcus insecticola Hs20B0-1T (=JCM 33485T=DSM 110147T) and Lactococcus hodotermopsidis Hs30E4-3T (=JCM 33486T=DSM 110148T), respectively.
32614762	6	86	theme	gene	668:671	arg1	sequences					673:681	16S rRNA gene and rpoB gene sequences	645:681	sequences	673:681	Strain Hs30E4-3T was related to Lactococcus plantarum DSM 20686T showing 94.8 and 82.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively.
32614762	7	87	dep	strains	744:750	arg1	strains					744:750	strains Hs20B0-1T and Hs30E4-3T	744:774	strains Hs20B0-1T and Hs30E4-3T	744:774	The 16S rRNA gene sequence similarity between strains Hs20B0-1T and Hs30E4-3T was 95.7 %.
32614762	7	87	dep	strains	744:750	arg1	Hs30E4-3T					766:774	Hs30E4-3T	766:774	Hs30E4-3T	766:774	The 16S rRNA gene sequence similarity between strains Hs20B0-1T and Hs30E4-3T was 95.7 %.
32614762	7	87	dep	strains	744:750	arg1	Hs20B0-1T					752:760	Hs20B0-1T	752:760	Hs20B0-1T	752:760	The 16S rRNA gene sequence similarity between strains Hs20B0-1T and Hs30E4-3T was 95.7 %.
32614762	11	88	dep	strains	1255:1261	arg1	strains					1255:1261	strains Hs20B0-1T and Hs30E4-3T	1255:1285	strains Hs20B0-1T and Hs30E4-3T	1255:1285	The cell-wall peptidoglycan types of strains Hs20B0-1T and Hs30E4-3T were A4α, Lys-Asp and A3α, Lys-Thr-Ala, respectively.
32614762	11	88	dep	strains	1255:1261	arg1	Hs30E4-3T					1277:1285	Hs30E4-3T	1277:1285	Hs30E4-3T	1277:1285	The cell-wall peptidoglycan types of strains Hs20B0-1T and Hs30E4-3T were A4α, Lys-Asp and A3α, Lys-Thr-Ala, respectively.
32614762	11	88	dep	strains	1255:1261	arg1	Hs20B0-1T					1263:1271	Hs20B0-1T	1263:1271	Hs20B0-1T	1263:1271	The cell-wall peptidoglycan types of strains Hs20B0-1T and Hs30E4-3T were A4α, Lys-Asp and A3α, Lys-Thr-Ala, respectively.
32614762	13	89	theme	insecticola	1589:1599	arg1	Hs20B0-1T					1601:1609	Lactococcus insecticola Hs20B0-1T	1577:1609	Lactococcus insecticola Hs20B0-1T (=JCM 33485T=DSM 110147T)	1577:1635	In conclusion, two novel species of the genus Lactococcus are proposed, namely Lactococcus insecticola Hs20B0-1T (=JCM 33485T=DSM 110147T) and Lactococcus hodotermopsidis Hs30E4-3T (=JCM 33486T=DSM 110148T), respectively.
32614762	13	89	theme	insecticola	1589:1599	arg1	110147T					1628:1634	=JCM 33485T=DSM 110147T	1612:1634	=JCM 33485T=DSM 110147T	1612:1634	In conclusion, two novel species of the genus Lactococcus are proposed, namely Lactococcus insecticola Hs20B0-1T (=JCM 33485T=DSM 110147T) and Lactococcus hodotermopsidis Hs30E4-3T (=JCM 33486T=DSM 110148T), respectively.
32614762	9	90	theme	plantarum	1097:1105	arg1	20686T					1111:1116	L. plantarum DSM 20686T	1094:1116	L. plantarum DSM 20686T	1094:1116	Strain Hs30E4-3T had 72.8 % ANI similarity and 21.3 % DDH similarity to L. plantarum DSM 20686T.
32614762	1	91	theme	nov.	28:31	arg1	hodotermopsidis					49:63	nov. and Lactococcus hodotermopsidis	28:63	nov. and Lactococcus hodotermopsidis	28:63	nov. and Lactococcus hodotermopsidis sp.
32614762	8	92	theme	strain	927:932	arg1	Hs20B0-1T					934:942	strain Hs20B0-1T	927:942	strain Hs20B0-1T	927:942	Furthermore, genomic comparisons using pairwise average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) analyses between strain Hs20B0-1T and L. piscium DSM 6634T resulted in values of 73.5 and 20.1 %, respectively.
32614762	5	93	theme	sequence	457:464	arg1	similarity					466:475	96.3 and 84.2 % sequence similarity	441:475	96.3 and 84.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively	441:530	Strain Hs20B0-1T was related to Lactococcus piscium DSM 6634T showing 96.3 and 84.2 % sequence similarity in 16S rRNA gene and rpoB gene sequences, respectively.
32614762	12	94	theme	other	1423:1427	arg1	species					1453:1459	each other and other established Lactococcus species	1408:1459	species	1453:1459	The two strains, Hs20B0-1T and Hs30E4-3T, are distinguishable from each other and other established Lactococcus species phylogenetically and phenotypically.
32614762	1	95	theme	Lactococcus	37:47	arg1	hodotermopsidis					49:63	nov. and Lactococcus hodotermopsidis	28:63	nov. and Lactococcus hodotermopsidis	28:63	nov. and Lactococcus hodotermopsidis sp.
32614762	3	96	attach	isolated	234:241	arg1	gut					252:254	the gut	248:254	the gut of the damp-wood termite Hodotermopsis sjostedti	248:303	Two strains of lactic acid bacteria, designated Hs20B0-1T and Hs30E4-3T, were isolated from the gut of the damp-wood termite Hodotermopsis sjostedti.
32614762	3	96	attach	isolated	234:241	arg2	strains					160:166	Two strains	156:166	Two strains	156:166	Two strains of lactic acid bacteria, designated Hs20B0-1T and Hs30E4-3T, were isolated from the gut of the damp-wood termite Hodotermopsis sjostedti.
32788375	14	0	theme	prebiotics	2379:2388	arg1	result					2398:2403	(i) dietary prebiotics (fiber) result	2367:2403	two important findings: (i) dietary prebiotics (fiber) result	2343:2403	Our research has two important findings: (i) dietary prebiotics (fiber) result in the microbiota from adolescents with obesity producing more SCFA, and (ii) the effectiveness of each prebiotic is donor dependent.
32788375	3	1	theme	metabolic	518:526	arg1	syndrome					528:535	metabolic syndrome	518:535	metabolic syndrome	518:535	Specifically, the microbial production of short-chain fatty acids (SCFA) from the fermentation of otherwise indigestible dietary carbohydrates may protect against pediatric obesity and metabolic syndrome.
32788375	8	2	theme	SCFA	1166:1169	arg1	production					1171:1180	SCFA production	1166:1180	SCFA production	1166:1180	Significant interdonor variation also existed in SCFA production, which 16S rRNA sequencing supported as being associated with differences in the host microbiota composition.
32788375	14	3	theme	more	2463:2466	arg1	SCFA					2468:2471	more SCFA	2463:2471	more SCFA	2463:2471	Our research has two important findings: (i) dietary prebiotics (fiber) result in the microbiota from adolescents with obesity producing more SCFA, and (ii) the effectiveness of each prebiotic is donor dependent.
32788375	5	4	dep	in	784:785	arg1	vitro					787:791	vitro	787:791	vitro	787:791	Here, we used an in vitro system to examine the SCFA production by fecal microbiota from 17 children with obesity when exposed to five different commercially available over-the-counter (OTC) prebiotic supplements.
32788375	11	5	theme	higher	1942:1947	arg1	risks					1956:1960	higher health risks	1942:1960	higher health risks	1942:1960	Children and adolescents with obesity face higher health risks, and noninvasive therapies for pediatric obesity often have limited success.
32788375	10	6	theme	SCFA	1579:1582	arg1	production					1584:1593	SCFA production	1579:1593	SCFA production	1579:1593	Together, these in vitro findings suggest the hypothesis that OTC prebiotic supplements may be unequal in their ability to stimulate SCFA production in children and adolescents with obesity and that the most acidogenic prebiotic may differ across individuals.IMPORTANCE Pediatric obesity remains a major public health problem in the United States, where 17% of children and adolescents are obese, and rates of pediatric "severe obesity" are increasing.
32788375	10	7	theme	United	1779:1784	arg1	States					1786:1791	the United States	1775:1791	the United States	1775:1791	Together, these in vitro findings suggest the hypothesis that OTC prebiotic supplements may be unequal in their ability to stimulate SCFA production in children and adolescents with obesity and that the most acidogenic prebiotic may differ across individuals.IMPORTANCE Pediatric obesity remains a major public health problem in the United States, where 17% of children and adolescents are obese, and rates of pediatric "severe obesity" are increasing.
32788375	11	8	contain	have	2017:2020	arg1	Children					1899:1906	Children	1899:1906	Children	1899:1906	Children and adolescents with obesity face higher health risks, and noninvasive therapies for pediatric obesity often have limited success.
32788375	11	8	contain	have	2017:2020	arg2	success					2030:2036	limited success	2022:2036	limited success	2022:2036	Children and adolescents with obesity face higher health risks, and noninvasive therapies for pediatric obesity often have limited success.
32788375	11	8	contain	have	2017:2020	arg1	therapies					1979:1987	noninvasive therapies	1967:1987	noninvasive therapies for pediatric obesity	1967:2009	Children and adolescents with obesity face higher health risks, and noninvasive therapies for pediatric obesity often have limited success.
32788375	11	8	contain	have	2017:2020	arg1	adolescents					1912:1922	adolescents	1912:1922	adolescents	1912:1922	Children and adolescents with obesity face higher health risks, and noninvasive therapies for pediatric obesity often have limited success.
32788375	4	9	theme	SCFA	683:686	arg1	production					688:697	gut bacterial SCFA production	669:697	gut bacterial SCFA production	669:697	Still, it has not been demonstrated that therapies involving microbiota-targeting carbohydrates, known as prebiotics, will enhance gut bacterial SCFA production in children and adolescents with obesity (age, 10 to 18 years old).
32788375	2	10	theme	causal	271:276	arg1	role					278:281	a causal role	269:281	a causal role	269:281	The gut microbiota plays a causal role in obesity and is a promising therapeutic target.
32788375	1	11	theme	public	182:187	arg1	burden					196:201	a public health burden	180:201	a public health burden	180:201	Pediatric obesity remains a public health burden and continues to increase in prevalence.
32788375	8	12	theme	16S	1189:1191	arg1	sequencing					1198:1207	16S rRNA sequencing	1189:1207	16S rRNA sequencing	1189:1207	Significant interdonor variation also existed in SCFA production, which 16S rRNA sequencing supported as being associated with differences in the host microbiota composition.
32788375	12	13	theme	weight	2144:2149	arg1	loss					2151:2154	weight loss	2144:2154	weight loss	2144:2154	The human gut microbiome has been implicated in adult obesity, and microbiota-directed therapies can aid weight loss in adults with obesity.
32788375	13	14	from	microbiome	2213:2222	arg1	obesity					2237:2243	pediatric obesity	2227:2243	pediatric obesity	2227:2243	However, less is known about the microbiome in pediatric obesity, and microbiota-directed therapies are understudied in children and adolescents.
32788375	15	15	theme	prebiotic	2577:2585	arg1	supplements					2587:2597	prebiotic supplements	2577:2597	prebiotic supplements	2577:2597	Together, these findings suggest that prebiotic supplements could help children and adolescents with obesity, but that these therapies may not be "one size fits all."
32788375	8	16	theme	microbiota	1268:1277	arg1	composition					1279:1289	the host microbiota composition	1259:1289	the host microbiota composition	1259:1289	Significant interdonor variation also existed in SCFA production, which 16S rRNA sequencing supported as being associated with differences in the host microbiota composition.
32788375	10	17	theme	major	1744:1748	arg1	problem					1764:1770	a major public health problem	1742:1770	a major public health problem	1742:1770	Together, these in vitro findings suggest the hypothesis that OTC prebiotic supplements may be unequal in their ability to stimulate SCFA production in children and adolescents with obesity and that the most acidogenic prebiotic may differ across individuals.IMPORTANCE Pediatric obesity remains a major public health problem in the United States, where 17% of children and adolescents are obese, and rates of pediatric "severe obesity" are increasing.
32788375	15	18	with	adolescents	2623:2633	arg1	obesity					2640:2646	obesity	2640:2646	obesity	2640:2646	Together, these findings suggest that prebiotic supplements could help children and adolescents with obesity, but that these therapies may not be "one size fits all."
32788375	4	19	theme	gut	669:671	arg1	production					688:697	gut bacterial SCFA production	669:697	gut bacterial SCFA production	669:697	Still, it has not been demonstrated that therapies involving microbiota-targeting carbohydrates, known as prebiotics, will enhance gut bacterial SCFA production in children and adolescents with obesity (age, 10 to 18 years old).
32788375	0	20	theme	Community	131:139	arg1	Composition					141:151	Bacterial Community Composition	121:151	Bacterial Community Composition	121:151	Short-Chain Fatty Acid Production by Gut Microbiota from Children with Obesity Differs According to Prebiotic Choice and Bacterial Community Composition.
32788375	0	21	from	Children	57:64	arg1	Production					23:32	Short-Chain Fatty Acid Production	0:32	Short-Chain Fatty Acid Production by Gut Microbiota from Children with Obesity	0:77	Short-Chain Fatty Acid Production by Gut Microbiota from Children with Obesity Differs According to Prebiotic Choice and Bacterial Community Composition.
32788375	11	22	theme	health	1949:1954	arg1	risks					1956:1960	higher health risks	1942:1960	higher health risks	1942:1960	Children and adolescents with obesity face higher health risks, and noninvasive therapies for pediatric obesity often have limited success.
32788375	8	23	from	differences	1244:1254	arg1	composition					1279:1289	the host microbiota composition	1259:1289	the host microbiota composition	1259:1289	Significant interdonor variation also existed in SCFA production, which 16S rRNA sequencing supported as being associated with differences in the host microbiota composition.
32788375	4	24	dep	18	752:753	arg1	to					749:750	to	749:750	to	749:750	Still, it has not been demonstrated that therapies involving microbiota-targeting carbohydrates, known as prebiotics, will enhance gut bacterial SCFA production in children and adolescents with obesity (age, 10 to 18 years old).
32788375	12	25	theme	adult	2087:2091	arg1	obesity					2093:2099	adult obesity	2087:2099	adult obesity	2087:2099	The human gut microbiome has been implicated in adult obesity, and microbiota-directed therapies can aid weight loss in adults with obesity.
32788375	3	26	theme	carbohydrates	462:474	arg1	fermentation					415:426	the fermentation	411:426	the fermentation of otherwise indigestible dietary carbohydrates	411:474	Specifically, the microbial production of short-chain fatty acids (SCFA) from the fermentation of otherwise indigestible dietary carbohydrates may protect against pediatric obesity and metabolic syndrome.
32788375	5	27	dep	available	925:933	arg1	prebiotic					958:966	prebiotic	958:966	prebiotic	958:966	Here, we used an in vitro system to examine the SCFA production by fecal microbiota from 17 children with obesity when exposed to five different commercially available over-the-counter (OTC) prebiotic supplements.
32788375	2	28	theme	gut	248:250	arg1	microbiota					252:261	The gut microbiota	244:261	The gut microbiota	244:261	The gut microbiota plays a causal role in obesity and is a promising therapeutic target.
32788375	1	29	theme	Pediatric	154:162	arg1	obesity					164:170	Pediatric obesity	154:170	Pediatric obesity	154:170	Pediatric obesity remains a public health burden and continues to increase in prevalence.
32788375	13	30	theme	microbiota-directed	2250:2268	arg1	therapies					2270:2278	microbiota-directed therapies	2250:2278	microbiota-directed therapies	2250:2278	However, less is known about the microbiome in pediatric obesity, and microbiota-directed therapies are understudied in children and adolescents.
32788375	3	31	theme	microbial	351:359	arg1	production					361:370	the microbial production	347:370	the microbial production of short-chain fatty acids (SCFA) from the fermentation of otherwise indigestible dietary carbohydrates	347:474	Specifically, the microbial production of short-chain fatty acids (SCFA) from the fermentation of otherwise indigestible dietary carbohydrates may protect against pediatric obesity and metabolic syndrome.
32788375	4	32	dep	adolescents	715:725	arg1	years					755:759	10 to 18 years	746:759	10 to 18 years old	746:763	Still, it has not been demonstrated that therapies involving microbiota-targeting carbohydrates, known as prebiotics, will enhance gut bacterial SCFA production in children and adolescents with obesity (age, 10 to 18 years old).
32788375	4	32	dep	adolescents	715:725	arg1	age					741:743	age	741:743	age	741:743	Still, it has not been demonstrated that therapies involving microbiota-targeting carbohydrates, known as prebiotics, will enhance gut bacterial SCFA production in children and adolescents with obesity (age, 10 to 18 years old).
32788375	0	33	theme	Prebiotic	100:108	arg1	Choice					110:115	Prebiotic Choice	100:115	Prebiotic Choice	100:115	Short-Chain Fatty Acid Production by Gut Microbiota from Children with Obesity Differs According to Prebiotic Choice and Bacterial Community Composition.
32788375	9	34	theme	fecal	1320:1324	arg1	concentration					1331:1343	neither fecal SCFA concentration	1312:1343	neither fecal SCFA concentration	1312:1343	Last, we found that neither fecal SCFA concentration, microbiota SCFA production capacity, nor markers of obesity positively correlated with one another.
32788375	10	35	dep	in	1462:1463	arg1	vitro					1465:1469	vitro	1465:1469	vitro	1465:1469	Together, these in vitro findings suggest the hypothesis that OTC prebiotic supplements may be unequal in their ability to stimulate SCFA production in children and adolescents with obesity and that the most acidogenic prebiotic may differ across individuals.IMPORTANCE Pediatric obesity remains a major public health problem in the United States, where 17% of children and adolescents are obese, and rates of pediatric "severe obesity" are increasing.
32788375	3	36	theme	fatty	387:391	arg1	SCFA					400:403	SCFA	400:403	SCFA	400:403	Specifically, the microbial production of short-chain fatty acids (SCFA) from the fermentation of otherwise indigestible dietary carbohydrates may protect against pediatric obesity and metabolic syndrome.
32788375	3	36	theme	fatty	387:391	arg1	acids					393:397	short-chain fatty acids	375:397	short-chain fatty acids (SCFA) from the fermentation of otherwise indigestible dietary carbohydrates	375:474	Specifically, the microbial production of short-chain fatty acids (SCFA) from the fermentation of otherwise indigestible dietary carbohydrates may protect against pediatric obesity and metabolic syndrome.
32788375	13	37	theme	pediatric	2227:2235	arg1	obesity					2237:2243	pediatric obesity	2227:2243	pediatric obesity	2227:2243	However, less is known about the microbiome in pediatric obesity, and microbiota-directed therapies are understudied in children and adolescents.
32788375	0	38	theme	Short-Chain	0:10	arg1	Acid					18:21	Short-Chain Fatty Acid	0:21	Short-Chain Fatty Acid Production by Gut Microbiota from Children with Obesity	0:77	Short-Chain Fatty Acid Production by Gut Microbiota from Children with Obesity Differs According to Prebiotic Choice and Bacterial Community Composition.
32788375	14	39	contain	has	2339:2341	arg1	research					2330:2337	Our research	2326:2337	Our research	2326:2337	Our research has two important findings: (i) dietary prebiotics (fiber) result in the microbiota from adolescents with obesity producing more SCFA, and (ii) the effectiveness of each prebiotic is donor dependent.
32788375	14	39	contain	has	2339:2341	arg2	findings					2357:2364	two important findings	2343:2364	two important findings: (i) dietary prebiotics (fiber) result	2343:2403	Our research has two important findings: (i) dietary prebiotics (fiber) result in the microbiota from adolescents with obesity producing more SCFA, and (ii) the effectiveness of each prebiotic is donor dependent.
32788375	10	40	theme	OTC	1508:1510	arg1	supplements					1522:1532	OTC prebiotic supplements	1508:1532	OTC prebiotic supplements	1508:1532	Together, these in vitro findings suggest the hypothesis that OTC prebiotic supplements may be unequal in their ability to stimulate SCFA production in children and adolescents with obesity and that the most acidogenic prebiotic may differ across individuals.IMPORTANCE Pediatric obesity remains a major public health problem in the United States, where 17% of children and adolescents are obese, and rates of pediatric "severe obesity" are increasing.
32788375	12	41	with	adults	2159:2164	arg1	obesity					2171:2177	obesity	2171:2177	obesity	2171:2177	The human gut microbiome has been implicated in adult obesity, and microbiota-directed therapies can aid weight loss in adults with obesity.
32788375	10	42	theme	individuals.IMPORTANCE	1693:1714	arg1	obesity					1726:1732	individuals.IMPORTANCE Pediatric obesity	1693:1732	individuals.IMPORTANCE Pediatric obesity	1693:1732	Together, these in vitro findings suggest the hypothesis that OTC prebiotic supplements may be unequal in their ability to stimulate SCFA production in children and adolescents with obesity and that the most acidogenic prebiotic may differ across individuals.IMPORTANCE Pediatric obesity remains a major public health problem in the United States, where 17% of children and adolescents are obese, and rates of pediatric "severe obesity" are increasing.
32788375	0	43	theme	Acid	18:21	arg1	Production					23:32	Short-Chain Fatty Acid Production	0:32	Short-Chain Fatty Acid Production by Gut Microbiota from Children with Obesity	0:77	Short-Chain Fatty Acid Production by Gut Microbiota from Children with Obesity Differs According to Prebiotic Choice and Bacterial Community Composition.
32788375	9	44	theme	microbiota	1346:1355	arg1	capacity					1373:1380	microbiota SCFA production capacity	1346:1380	microbiota SCFA production capacity	1346:1380	Last, we found that neither fecal SCFA concentration, microbiota SCFA production capacity, nor markers of obesity positively correlated with one another.
32788375	5	45	used	used	776:779	arg2	we					773:774	we	773:774	we	773:774	Here, we used an in vitro system to examine the SCFA production by fecal microbiota from 17 children with obesity when exposed to five different commercially available over-the-counter (OTC) prebiotic supplements.
32788375	14	46	theme	prebiotic	2509:2517	arg1	dependent					2528:2536	dependent	2528:2536	dependent	2528:2536	Our research has two important findings: (i) dietary prebiotics (fiber) result in the microbiota from adolescents with obesity producing more SCFA, and (ii) the effectiveness of each prebiotic is donor dependent.
32788375	14	46	theme	prebiotic	2509:2517	arg1	ii					2479:2480	ii	2479:2480	ii	2479:2480	Our research has two important findings: (i) dietary prebiotics (fiber) result in the microbiota from adolescents with obesity producing more SCFA, and (ii) the effectiveness of each prebiotic is donor dependent.
32788375	14	46	theme	prebiotic	2509:2517	arg1	effectiveness					2487:2499	the effectiveness	2483:2499	the effectiveness of each prebiotic	2483:2517	Our research has two important findings: (i) dietary prebiotics (fiber) result in the microbiota from adolescents with obesity producing more SCFA, and (ii) the effectiveness of each prebiotic is donor dependent.
32788375	12	47	theme	gut	2049:2051	arg1	microbiome					2053:2062	The human gut microbiome	2039:2062	The human gut microbiome	2039:2062	The human gut microbiome has been implicated in adult obesity, and microbiota-directed therapies can aid weight loss in adults with obesity.
32788375	10	48	theme	pediatric	1856:1864	arg1	"					1881:1881	pediatric "severe obesity"	1856:1881	pediatric "severe obesity"	1856:1881	Together, these in vitro findings suggest the hypothesis that OTC prebiotic supplements may be unequal in their ability to stimulate SCFA production in children and adolescents with obesity and that the most acidogenic prebiotic may differ across individuals.IMPORTANCE Pediatric obesity remains a major public health problem in the United States, where 17% of children and adolescents are obese, and rates of pediatric "severe obesity" are increasing.
32788375	3	49	theme	indigestible	441:452	arg1	carbohydrates					462:474	otherwise indigestible dietary carbohydrates	431:474	otherwise indigestible dietary carbohydrates	431:474	Specifically, the microbial production of short-chain fatty acids (SCFA) from the fermentation of otherwise indigestible dietary carbohydrates may protect against pediatric obesity and metabolic syndrome.
32788375	9	50	theme	production	1362:1371	arg1	capacity					1373:1380	microbiota SCFA production capacity	1346:1380	microbiota SCFA production capacity	1346:1380	Last, we found that neither fecal SCFA concentration, microbiota SCFA production capacity, nor markers of obesity positively correlated with one another.
32788375	10	51	from	ability	1558:1564	arg1	unequal					1541:1547	unequal	1541:1547	unequal	1541:1547	Together, these in vitro findings suggest the hypothesis that OTC prebiotic supplements may be unequal in their ability to stimulate SCFA production in children and adolescents with obesity and that the most acidogenic prebiotic may differ across individuals.IMPORTANCE Pediatric obesity remains a major public health problem in the United States, where 17% of children and adolescents are obese, and rates of pediatric "severe obesity" are increasing.
32788375	10	52	with	children	1598:1605	arg1	obesity					1628:1634	obesity	1628:1634	obesity	1628:1634	Together, these in vitro findings suggest the hypothesis that OTC prebiotic supplements may be unequal in their ability to stimulate SCFA production in children and adolescents with obesity and that the most acidogenic prebiotic may differ across individuals.IMPORTANCE Pediatric obesity remains a major public health problem in the United States, where 17% of children and adolescents are obese, and rates of pediatric "severe obesity" are increasing.
32788375	3	53	theme	pediatric	496:504	arg1	obesity					506:512	pediatric obesity	496:512	pediatric obesity	496:512	Specifically, the microbial production of short-chain fatty acids (SCFA) from the fermentation of otherwise indigestible dietary carbohydrates may protect against pediatric obesity and metabolic syndrome.
32788375	10	54	with	adolescents	1611:1621	arg1	obesity					1628:1634	obesity	1628:1634	obesity	1628:1634	Together, these in vitro findings suggest the hypothesis that OTC prebiotic supplements may be unequal in their ability to stimulate SCFA production in children and adolescents with obesity and that the most acidogenic prebiotic may differ across individuals.IMPORTANCE Pediatric obesity remains a major public health problem in the United States, where 17% of children and adolescents are obese, and rates of pediatric "severe obesity" are increasing.
32788375	11	55	theme	noninvasive	1967:1977	arg1	therapies					1979:1987	noninvasive therapies	1967:1987	noninvasive therapies for pediatric obesity	1967:2009	Children and adolescents with obesity face higher health risks, and noninvasive therapies for pediatric obesity often have limited success.
32788375	10	56	theme	"	1881:1881	arg1	rates					1847:1851	rates	1847:1851	rates of pediatric "severe obesity"	1847:1881	Together, these in vitro findings suggest the hypothesis that OTC prebiotic supplements may be unequal in their ability to stimulate SCFA production in children and adolescents with obesity and that the most acidogenic prebiotic may differ across individuals.IMPORTANCE Pediatric obesity remains a major public health problem in the United States, where 17% of children and adolescents are obese, and rates of pediatric "severe obesity" are increasing.
32788375	15	57	with	children	2610:2617	arg1	obesity					2640:2646	obesity	2640:2646	obesity	2640:2646	Together, these findings suggest that prebiotic supplements could help children and adolescents with obesity, but that these therapies may not be "one size fits all."
32788375	10	58	theme	severe	1867:1872	arg1	"					1881:1881	pediatric "severe obesity"	1856:1881	pediatric "severe obesity"	1856:1881	Together, these in vitro findings suggest the hypothesis that OTC prebiotic supplements may be unequal in their ability to stimulate SCFA production in children and adolescents with obesity and that the most acidogenic prebiotic may differ across individuals.IMPORTANCE Pediatric obesity remains a major public health problem in the United States, where 17% of children and adolescents are obese, and rates of pediatric "severe obesity" are increasing.
32788375	3	59	from	fermentation	415:426	arg1	SCFA					400:403	SCFA	400:403	SCFA	400:403	Specifically, the microbial production of short-chain fatty acids (SCFA) from the fermentation of otherwise indigestible dietary carbohydrates may protect against pediatric obesity and metabolic syndrome.
32788375	3	59	from	fermentation	415:426	arg1	acids					393:397	short-chain fatty acids	375:397	short-chain fatty acids (SCFA) from the fermentation of otherwise indigestible dietary carbohydrates	375:474	Specifically, the microbial production of short-chain fatty acids (SCFA) from the fermentation of otherwise indigestible dietary carbohydrates may protect against pediatric obesity and metabolic syndrome.
32788375	3	59	from	fermentation	415:426	arg1	production					361:370	the microbial production	347:370	the microbial production of short-chain fatty acids (SCFA) from the fermentation of otherwise indigestible dietary carbohydrates	347:474	Specifically, the microbial production of short-chain fatty acids (SCFA) from the fermentation of otherwise indigestible dietary carbohydrates may protect against pediatric obesity and metabolic syndrome.
32788375	5	60	theme	fecal	834:838	arg1	microbiota					840:849	fecal microbiota	834:849	fecal microbiota	834:849	Here, we used an in vitro system to examine the SCFA production by fecal microbiota from 17 children with obesity when exposed to five different commercially available over-the-counter (OTC) prebiotic supplements.
32788375	9	61	theme	obesity	1398:1404	arg1	markers					1387:1393	markers	1387:1393	markers of obesity	1387:1404	Last, we found that neither fecal SCFA concentration, microbiota SCFA production capacity, nor markers of obesity positively correlated with one another.
32788375	8	62	theme	interdonor	1129:1138	arg1	variation					1140:1148	Significant interdonor variation	1117:1148	Significant interdonor variation	1117:1148	Significant interdonor variation also existed in SCFA production, which 16S rRNA sequencing supported as being associated with differences in the host microbiota composition.
32788375	14	63	dep	dependent	2528:2536	arg1	dependent					2528:2536	dependent	2528:2536	dependent	2528:2536	Our research has two important findings: (i) dietary prebiotics (fiber) result in the microbiota from adolescents with obesity producing more SCFA, and (ii) the effectiveness of each prebiotic is donor dependent.
32788375	14	63	dep	dependent	2528:2536	arg1	ii					2479:2480	ii	2479:2480	ii	2479:2480	Our research has two important findings: (i) dietary prebiotics (fiber) result in the microbiota from adolescents with obesity producing more SCFA, and (ii) the effectiveness of each prebiotic is donor dependent.
32788375	14	63	dep	dependent	2528:2536	arg1	effectiveness					2487:2499	the effectiveness	2483:2499	the effectiveness of each prebiotic	2483:2517	Our research has two important findings: (i) dietary prebiotics (fiber) result in the microbiota from adolescents with obesity producing more SCFA, and (ii) the effectiveness of each prebiotic is donor dependent.
32788375	10	64	theme	public	1750:1755	arg1	problem					1764:1770	a major public health problem	1742:1770	a major public health problem	1742:1770	Together, these in vitro findings suggest the hypothesis that OTC prebiotic supplements may be unequal in their ability to stimulate SCFA production in children and adolescents with obesity and that the most acidogenic prebiotic may differ across individuals.IMPORTANCE Pediatric obesity remains a major public health problem in the United States, where 17% of children and adolescents are obese, and rates of pediatric "severe obesity" are increasing.
32788375	7	65	theme	acidogenic	1095:1104	arg1	potential					1106:1114	their acidogenic potential	1089:1114	their acidogenic potential	1089:1114	Still, supplements varied in their acidogenic potential.
32788375	14	66	theme	dietary	2371:2377	arg1	result					2398:2403	(i) dietary prebiotics (fiber) result	2367:2403	two important findings: (i) dietary prebiotics (fiber) result	2343:2403	Our research has two important findings: (i) dietary prebiotics (fiber) result in the microbiota from adolescents with obesity producing more SCFA, and (ii) the effectiveness of each prebiotic is donor dependent.
32788375	4	67	theme	microbiota-targeting	599:618	arg1	carbohydrates					620:632	microbiota-targeting carbohydrates	599:632	microbiota-targeting carbohydrates	599:632	Still, it has not been demonstrated that therapies involving microbiota-targeting carbohydrates, known as prebiotics, will enhance gut bacterial SCFA production in children and adolescents with obesity (age, 10 to 18 years old).
32788375	4	68	with	adolescents	715:725	arg1	obesity					732:738	obesity	732:738	obesity	732:738	Still, it has not been demonstrated that therapies involving microbiota-targeting carbohydrates, known as prebiotics, will enhance gut bacterial SCFA production in children and adolescents with obesity (age, 10 to 18 years old).
32788375	0	69	with	Children	57:64	arg1	Obesity					71:77	Obesity	71:77	Obesity	71:77	Short-Chain Fatty Acid Production by Gut Microbiota from Children with Obesity Differs According to Prebiotic Choice and Bacterial Community Composition.
32788375	11	70	theme	pediatric	1993:2001	arg1	obesity					2003:2009	pediatric obesity	1993:2009	pediatric obesity	1993:2009	Children and adolescents with obesity face higher health risks, and noninvasive therapies for pediatric obesity often have limited success.
32788375	4	71	theme	bacterial	673:681	arg1	production					688:697	gut bacterial SCFA production	669:697	gut bacterial SCFA production	669:697	Still, it has not been demonstrated that therapies involving microbiota-targeting carbohydrates, known as prebiotics, will enhance gut bacterial SCFA production in children and adolescents with obesity (age, 10 to 18 years old).
32788375	4	72	with	children	702:709	arg1	obesity					732:738	obesity	732:738	obesity	732:738	Still, it has not been demonstrated that therapies involving microbiota-targeting carbohydrates, known as prebiotics, will enhance gut bacterial SCFA production in children and adolescents with obesity (age, 10 to 18 years old).
32788375	6	73	from	patients	1013:1020	arg1	microbiota					990:999	microbiota	990:999	microbiota from all 17 patients actively metabolized most prebiotics	990:1057	We found microbiota from all 17 patients actively metabolized most prebiotics.
32788375	2	74	theme	promising	303:311	arg1	target					325:330	a promising therapeutic target	301:330	a promising therapeutic target	301:330	The gut microbiota plays a causal role in obesity and is a promising therapeutic target.
32788375	1	75	theme	health	189:194	arg1	burden					196:201	a public health burden	180:201	a public health burden	180:201	Pediatric obesity remains a public health burden and continues to increase in prevalence.
32788375	0	76	theme	Bacterial	121:129	arg1	Composition					141:151	Bacterial Community Composition	121:151	Bacterial Community Composition	121:151	Short-Chain Fatty Acid Production by Gut Microbiota from Children with Obesity Differs According to Prebiotic Choice and Bacterial Community Composition.
32788375	14	77	theme	important	2347:2355	arg1	findings					2357:2364	two important findings	2343:2364	two important findings: (i) dietary prebiotics (fiber) result	2343:2403	Our research has two important findings: (i) dietary prebiotics (fiber) result in the microbiota from adolescents with obesity producing more SCFA, and (ii) the effectiveness of each prebiotic is donor dependent.
32788375	11	78	theme	limited	2022:2028	arg1	success					2030:2036	limited success	2022:2036	limited success	2022:2036	Children and adolescents with obesity face higher health risks, and noninvasive therapies for pediatric obesity often have limited success.
32788375	8	79	theme	rRNA	1193:1196	arg1	sequencing					1198:1207	16S rRNA sequencing	1189:1207	16S rRNA sequencing	1189:1207	Significant interdonor variation also existed in SCFA production, which 16S rRNA sequencing supported as being associated with differences in the host microbiota composition.
32788375	8	80	theme	host	1263:1266	arg1	composition					1279:1289	the host microbiota composition	1259:1289	the host microbiota composition	1259:1289	Significant interdonor variation also existed in SCFA production, which 16S rRNA sequencing supported as being associated with differences in the host microbiota composition.
32788375	10	81	theme	adolescents	1820:1830	arg1	adolescents					1820:1830	adolescents	1820:1830	adolescents	1820:1830	Together, these in vitro findings suggest the hypothesis that OTC prebiotic supplements may be unequal in their ability to stimulate SCFA production in children and adolescents with obesity and that the most acidogenic prebiotic may differ across individuals.IMPORTANCE Pediatric obesity remains a major public health problem in the United States, where 17% of children and adolescents are obese, and rates of pediatric "severe obesity" are increasing.
32788375	10	81	theme	adolescents	1820:1830	arg1	children					1807:1814	children	1807:1814	children	1807:1814	Together, these in vitro findings suggest the hypothesis that OTC prebiotic supplements may be unequal in their ability to stimulate SCFA production in children and adolescents with obesity and that the most acidogenic prebiotic may differ across individuals.IMPORTANCE Pediatric obesity remains a major public health problem in the United States, where 17% of children and adolescents are obese, and rates of pediatric "severe obesity" are increasing.
32788375	10	81	theme	adolescents	1820:1830	arg1	%					1802:1802	17%	1800:1802	17% of children and adolescents	1800:1830	Together, these in vitro findings suggest the hypothesis that OTC prebiotic supplements may be unequal in their ability to stimulate SCFA production in children and adolescents with obesity and that the most acidogenic prebiotic may differ across individuals.IMPORTANCE Pediatric obesity remains a major public health problem in the United States, where 17% of children and adolescents are obese, and rates of pediatric "severe obesity" are increasing.
32788375	5	82	theme	SCFA	815:818	arg1	production					820:829	the SCFA production	811:829	the SCFA production by fecal microbiota from 17 children with obesity	811:879	Here, we used an in vitro system to examine the SCFA production by fecal microbiota from 17 children with obesity when exposed to five different commercially available over-the-counter (OTC) prebiotic supplements.
32788375	3	83	from	production	361:370	arg1	fermentation					415:426	the fermentation	411:426	the fermentation of otherwise indigestible dietary carbohydrates	411:474	Specifically, the microbial production of short-chain fatty acids (SCFA) from the fermentation of otherwise indigestible dietary carbohydrates may protect against pediatric obesity and metabolic syndrome.
32788375	10	84	theme	children	1807:1814	arg1	adolescents					1820:1830	adolescents	1820:1830	adolescents	1820:1830	Together, these in vitro findings suggest the hypothesis that OTC prebiotic supplements may be unequal in their ability to stimulate SCFA production in children and adolescents with obesity and that the most acidogenic prebiotic may differ across individuals.IMPORTANCE Pediatric obesity remains a major public health problem in the United States, where 17% of children and adolescents are obese, and rates of pediatric "severe obesity" are increasing.
32788375	10	84	theme	children	1807:1814	arg1	children					1807:1814	children	1807:1814	children	1807:1814	Together, these in vitro findings suggest the hypothesis that OTC prebiotic supplements may be unequal in their ability to stimulate SCFA production in children and adolescents with obesity and that the most acidogenic prebiotic may differ across individuals.IMPORTANCE Pediatric obesity remains a major public health problem in the United States, where 17% of children and adolescents are obese, and rates of pediatric "severe obesity" are increasing.
32788375	10	84	theme	children	1807:1814	arg1	%					1802:1802	17%	1800:1802	17% of children and adolescents	1800:1830	Together, these in vitro findings suggest the hypothesis that OTC prebiotic supplements may be unequal in their ability to stimulate SCFA production in children and adolescents with obesity and that the most acidogenic prebiotic may differ across individuals.IMPORTANCE Pediatric obesity remains a major public health problem in the United States, where 17% of children and adolescents are obese, and rates of pediatric "severe obesity" are increasing.
32788375	10	85	theme	health	1757:1762	arg1	problem					1764:1770	a major public health problem	1742:1770	a major public health problem	1742:1770	Together, these in vitro findings suggest the hypothesis that OTC prebiotic supplements may be unequal in their ability to stimulate SCFA production in children and adolescents with obesity and that the most acidogenic prebiotic may differ across individuals.IMPORTANCE Pediatric obesity remains a major public health problem in the United States, where 17% of children and adolescents are obese, and rates of pediatric "severe obesity" are increasing.
32788375	11	86	with	adolescents	1912:1922	arg1	obesity					1929:1935	obesity	1929:1935	obesity face higher health risks	1929:1960	Children and adolescents with obesity face higher health risks, and noninvasive therapies for pediatric obesity often have limited success.
32788375	9	87	dep	one	1433:1435	arg1	another					1437:1443	another	1437:1443	another	1437:1443	Last, we found that neither fecal SCFA concentration, microbiota SCFA production capacity, nor markers of obesity positively correlated with one another.
32788375	14	88	dep	result	2398:2403	arg1	i					2368:2368	i	2368:2368	i	2368:2368	Our research has two important findings: (i) dietary prebiotics (fiber) result in the microbiota from adolescents with obesity producing more SCFA, and (ii) the effectiveness of each prebiotic is donor dependent.
32788375	11	89	with	Children	1899:1906	arg1	obesity					1929:1935	obesity	1929:1935	obesity face higher health risks	1929:1960	Children and adolescents with obesity face higher health risks, and noninvasive therapies for pediatric obesity often have limited success.
32788375	14	90	with	adolescents	2428:2438	arg1	obesity					2445:2451	obesity	2445:2451	obesity producing more SCFA	2445:2471	Our research has two important findings: (i) dietary prebiotics (fiber) result in the microbiota from adolescents with obesity producing more SCFA, and (ii) the effectiveness of each prebiotic is donor dependent.
32788375	12	91	theme	human	2043:2047	arg1	microbiome					2053:2062	The human gut microbiome	2039:2062	The human gut microbiome	2039:2062	The human gut microbiome has been implicated in adult obesity, and microbiota-directed therapies can aid weight loss in adults with obesity.
32788375	11	92	with	therapies	1979:1987	arg1	obesity					1929:1935	obesity	1929:1935	obesity face higher health risks	1929:1960	Children and adolescents with obesity face higher health risks, and noninvasive therapies for pediatric obesity often have limited success.
32788375	15	93	dep	size	2690:2693	arg1	fits					2695:2698	fits	2695:2698	fits all	2695:2702	Together, these findings suggest that prebiotic supplements could help children and adolescents with obesity, but that these therapies may not be "one size fits all."
32788375	14	94	dep	findings	2357:2364	arg1	result					2398:2403	(i) dietary prebiotics (fiber) result	2367:2403	two important findings: (i) dietary prebiotics (fiber) result	2343:2403	Our research has two important findings: (i) dietary prebiotics (fiber) result in the microbiota from adolescents with obesity producing more SCFA, and (ii) the effectiveness of each prebiotic is donor dependent.
32788375	12	95	theme	microbiota-directed	2106:2124	arg1	therapies					2126:2134	microbiota-directed therapies	2106:2134	microbiota-directed therapies	2106:2134	The human gut microbiome has been implicated in adult obesity, and microbiota-directed therapies can aid weight loss in adults with obesity.
32788375	5	96	with	children	859:866	arg1	obesity					873:879	obesity	873:879	obesity	873:879	Here, we used an in vitro system to examine the SCFA production by fecal microbiota from 17 children with obesity when exposed to five different commercially available over-the-counter (OTC) prebiotic supplements.
32788375	4	97	theme	old	761:763	arg1	years					755:759	10 to 18 years	746:759	10 to 18 years old	746:763	Still, it has not been demonstrated that therapies involving microbiota-targeting carbohydrates, known as prebiotics, will enhance gut bacterial SCFA production in children and adolescents with obesity (age, 10 to 18 years old).
32788375	4	97	theme	old	761:763	arg1	age					741:743	age	741:743	age	741:743	Still, it has not been demonstrated that therapies involving microbiota-targeting carbohydrates, known as prebiotics, will enhance gut bacterial SCFA production in children and adolescents with obesity (age, 10 to 18 years old).
32788375	2	98	theme	therapeutic	313:323	arg1	target					325:330	a promising therapeutic target	301:330	a promising therapeutic target	301:330	The gut microbiota plays a causal role in obesity and is a promising therapeutic target.
32788375	5	99	from	children	859:866	arg1	production					820:829	the SCFA production	811:829	the SCFA production by fecal microbiota from 17 children with obesity	811:879	Here, we used an in vitro system to examine the SCFA production by fecal microbiota from 17 children with obesity when exposed to five different commercially available over-the-counter (OTC) prebiotic supplements.
32788375	14	100	from	adolescents	2428:2438	arg1	microbiota					2412:2421	the microbiota	2408:2421	the microbiota from adolescents with obesity producing more SCFA	2408:2471	Our research has two important findings: (i) dietary prebiotics (fiber) result in the microbiota from adolescents with obesity producing more SCFA, and (ii) the effectiveness of each prebiotic is donor dependent.
32788375	10	101	theme	acidogenic	1654:1663	arg1	prebiotic					1665:1673	the most acidogenic prebiotic	1645:1673	the most acidogenic prebiotic	1645:1673	Together, these in vitro findings suggest the hypothesis that OTC prebiotic supplements may be unequal in their ability to stimulate SCFA production in children and adolescents with obesity and that the most acidogenic prebiotic may differ across individuals.IMPORTANCE Pediatric obesity remains a major public health problem in the United States, where 17% of children and adolescents are obese, and rates of pediatric "severe obesity" are increasing.
32788375	5	102	theme	in	784:785	arg1	system					793:798	an in vitro system	781:798	an in vitro system	781:798	Here, we used an in vitro system to examine the SCFA production by fecal microbiota from 17 children with obesity when exposed to five different commercially available over-the-counter (OTC) prebiotic supplements.
32788375	9	103	theme	SCFA	1326:1329	arg1	concentration					1331:1343	neither fecal SCFA concentration	1312:1343	neither fecal SCFA concentration	1312:1343	Last, we found that neither fecal SCFA concentration, microbiota SCFA production capacity, nor markers of obesity positively correlated with one another.
32788375	5	104	theme	different	902:910	arg1	supplements					968:978	five different commercially available over-the-counter (OTC) prebiotic supplements	897:978	five different commercially available over-the-counter (OTC) prebiotic supplements	897:978	Here, we used an in vitro system to examine the SCFA production by fecal microbiota from 17 children with obesity when exposed to five different commercially available over-the-counter (OTC) prebiotic supplements.
32788375	3	105	theme	acids	393:397	arg1	production					361:370	the microbial production	347:370	the microbial production of short-chain fatty acids (SCFA) from the fermentation of otherwise indigestible dietary carbohydrates	347:474	Specifically, the microbial production of short-chain fatty acids (SCFA) from the fermentation of otherwise indigestible dietary carbohydrates may protect against pediatric obesity and metabolic syndrome.
32788375	10	106	theme	in	1462:1463	arg1	findings					1471:1478	these in vitro findings	1456:1478	these in vitro findings	1456:1478	Together, these in vitro findings suggest the hypothesis that OTC prebiotic supplements may be unequal in their ability to stimulate SCFA production in children and adolescents with obesity and that the most acidogenic prebiotic may differ across individuals.IMPORTANCE Pediatric obesity remains a major public health problem in the United States, where 17% of children and adolescents are obese, and rates of pediatric "severe obesity" are increasing.
32788375	10	107	dep	suggest	1480:1486	arg1	remains					1734:1740	remains	1734:1740	remains	1734:1740	Together, these in vitro findings suggest the hypothesis that OTC prebiotic supplements may be unequal in their ability to stimulate SCFA production in children and adolescents with obesity and that the most acidogenic prebiotic may differ across individuals.IMPORTANCE Pediatric obesity remains a major public health problem in the United States, where 17% of children and adolescents are obese, and rates of pediatric "severe obesity" are increasing.
32788375	5	108	theme	available	925:933	arg1	supplements					968:978	five different commercially available over-the-counter (OTC) prebiotic supplements	897:978	five different commercially available over-the-counter (OTC) prebiotic supplements	897:978	Here, we used an in vitro system to examine the SCFA production by fecal microbiota from 17 children with obesity when exposed to five different commercially available over-the-counter (OTC) prebiotic supplements.
32788375	6	109	theme	most	1043:1046	arg1	prebiotics					1048:1057	most prebiotics	1043:1057	most prebiotics	1043:1057	We found microbiota from all 17 patients actively metabolized most prebiotics.
32788375	3	110	theme	short-chain	375:385	arg1	SCFA					400:403	SCFA	400:403	SCFA	400:403	Specifically, the microbial production of short-chain fatty acids (SCFA) from the fermentation of otherwise indigestible dietary carbohydrates may protect against pediatric obesity and metabolic syndrome.
32788375	3	110	theme	short-chain	375:385	arg1	acids					393:397	short-chain fatty acids	375:397	short-chain fatty acids (SCFA) from the fermentation of otherwise indigestible dietary carbohydrates	375:474	Specifically, the microbial production of short-chain fatty acids (SCFA) from the fermentation of otherwise indigestible dietary carbohydrates may protect against pediatric obesity and metabolic syndrome.
32788375	0	111	theme	Gut	37:39	arg1	Microbiota					41:50	Gut Microbiota	37:50	Gut Microbiota	37:50	Short-Chain Fatty Acid Production by Gut Microbiota from Children with Obesity Differs According to Prebiotic Choice and Bacterial Community Composition.
32788375	9	112	theme	SCFA	1357:1360	arg1	capacity					1373:1380	microbiota SCFA production capacity	1346:1380	microbiota SCFA production capacity	1346:1380	Last, we found that neither fecal SCFA concentration, microbiota SCFA production capacity, nor markers of obesity positively correlated with one another.
32788375	10	113	from	unequal	1541:1547	arg1	ability					1558:1564	their ability to stimulate SCFA production in children and adolescents with obesity	1552:1634	their ability to stimulate SCFA production in children and adolescents with obesity	1552:1634	Together, these in vitro findings suggest the hypothesis that OTC prebiotic supplements may be unequal in their ability to stimulate SCFA production in children and adolescents with obesity and that the most acidogenic prebiotic may differ across individuals.IMPORTANCE Pediatric obesity remains a major public health problem in the United States, where 17% of children and adolescents are obese, and rates of pediatric "severe obesity" are increasing.
32788375	3	114	theme	dietary	454:460	arg1	carbohydrates					462:474	otherwise indigestible dietary carbohydrates	431:474	otherwise indigestible dietary carbohydrates	431:474	Specifically, the microbial production of short-chain fatty acids (SCFA) from the fermentation of otherwise indigestible dietary carbohydrates may protect against pediatric obesity and metabolic syndrome.
32788375	8	115	theme	Significant	1117:1127	arg1	variation					1140:1148	Significant interdonor variation	1117:1148	Significant interdonor variation	1117:1148	Significant interdonor variation also existed in SCFA production, which 16S rRNA sequencing supported as being associated with differences in the host microbiota composition.
32788375	10	116	theme	prebiotic	1512:1520	arg1	supplements					1522:1532	OTC prebiotic supplements	1508:1532	OTC prebiotic supplements	1508:1532	Together, these in vitro findings suggest the hypothesis that OTC prebiotic supplements may be unequal in their ability to stimulate SCFA production in children and adolescents with obesity and that the most acidogenic prebiotic may differ across individuals.IMPORTANCE Pediatric obesity remains a major public health problem in the United States, where 17% of children and adolescents are obese, and rates of pediatric "severe obesity" are increasing.
32788375	10	117	theme	obesity	1874:1880	arg1	"					1881:1881	pediatric "severe obesity"	1856:1881	pediatric "severe obesity"	1856:1881	Together, these in vitro findings suggest the hypothesis that OTC prebiotic supplements may be unequal in their ability to stimulate SCFA production in children and adolescents with obesity and that the most acidogenic prebiotic may differ across individuals.IMPORTANCE Pediatric obesity remains a major public health problem in the United States, where 17% of children and adolescents are obese, and rates of pediatric "severe obesity" are increasing.
32788375	10	118	theme	Pediatric	1716:1724	arg1	obesity					1726:1732	individuals.IMPORTANCE Pediatric obesity	1693:1732	individuals.IMPORTANCE Pediatric obesity	1693:1732	Together, these in vitro findings suggest the hypothesis that OTC prebiotic supplements may be unequal in their ability to stimulate SCFA production in children and adolescents with obesity and that the most acidogenic prebiotic may differ across individuals.IMPORTANCE Pediatric obesity remains a major public health problem in the United States, where 17% of children and adolescents are obese, and rates of pediatric "severe obesity" are increasing.
32788375	11	119	dep	obesity	1929:1935	arg1	face					1937:1940	face	1937:1940	face	1937:1940	Children and adolescents with obesity face higher health risks, and noninvasive therapies for pediatric obesity often have limited success.
32788375	3	120	theme	otherwise	431:439	arg1	carbohydrates					462:474	otherwise indigestible dietary carbohydrates	431:474	otherwise indigestible dietary carbohydrates	431:474	Specifically, the microbial production of short-chain fatty acids (SCFA) from the fermentation of otherwise indigestible dietary carbohydrates may protect against pediatric obesity and metabolic syndrome.
32788375	14	121	theme	fiber	2391:2395	arg1	result					2398:2403	(i) dietary prebiotics (fiber) result	2367:2403	two important findings: (i) dietary prebiotics (fiber) result	2343:2403	Our research has two important findings: (i) dietary prebiotics (fiber) result in the microbiota from adolescents with obesity producing more SCFA, and (ii) the effectiveness of each prebiotic is donor dependent.
32788375	0	122	theme	Fatty	12:16	arg1	Acid					18:21	Short-Chain Fatty Acid	0:21	Short-Chain Fatty Acid Production by Gut Microbiota from Children with Obesity	0:77	Short-Chain Fatty Acid Production by Gut Microbiota from Children with Obesity Differs According to Prebiotic Choice and Bacterial Community Composition.
34416957	7	0	theme	study	1138:1142	arg1	results					1122:1128	the results	1118:1128	the results of this study	1118:1142	From the results of this study, the addition of solutes and organic compounds may increase the tolerance of E. coli O157:H7 to acetic, lactic, and propionic acid treatments and that the salt or sucrose significantly affects cell MFA composition.
34416957	6	1	theme	MFA	955:957	arg1	composition					960:970	the membrane fatty acid (MFA) composition	930:970	the membrane fatty acid (MFA) composition of cells	930:979	From a result of evaluating the membrane fatty acid (MFA) composition of cells, salt or sucrose significantly increased levels of saturated fatty acids (SFAs) or SFAs and cyclopropane fatty acids, respectively.
34416957	6	2	theme	acid	949:952	arg1	composition					960:970	the membrane fatty acid (MFA) composition	930:970	the membrane fatty acid (MFA) composition of cells	930:979	From a result of evaluating the membrane fatty acid (MFA) composition of cells, salt or sucrose significantly increased levels of saturated fatty acids (SFAs) or SFAs and cyclopropane fatty acids, respectively.
34416957	3	3	theme	%	559:559	arg1	salt					561:564	3% salt	558:564	3% salt	558:564	For treatment of 1% malic acid, the addition of 3% salt showed synergistic effect.
34416957	6	4	theme	membrane	934:941	arg1	composition					960:970	the membrane fatty acid (MFA) composition	930:970	the membrane fatty acid (MFA) composition of cells	930:979	From a result of evaluating the membrane fatty acid (MFA) composition of cells, salt or sucrose significantly increased levels of saturated fatty acids (SFAs) or SFAs and cyclopropane fatty acids, respectively.
34416957	4	5	theme	%	659:659	arg1	acid					668:671	1% acetic acid	658:671	1% acetic acid	658:671	Whereas, when 3% salt, glucose, glycine, or sucrose was added to 1% acetic acid, the solutes antagonized the action of the acid against E. coli O157:H7.
34416957	1	6	theme	combinations	152:163	arg1	effects					141:147	the effects	137:147	the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth	137:308	This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth.
34416957	2	7	theme	different	438:446	arg1	values					463:468	different water activity values	438:468	different water activity values against E. coli O157:H7	438:492	Additionally, the effectiveness of combining organic acids and various concentrations of salt (0-18%) or sucrose (0-100%) with different water activity values against E. coli O157:H7 were evaluated.
34416957	4	8	dep	O157	737:740	arg1	H7					742:743	H7	742:743	E. coli O157:H7	729:743	Whereas, when 3% salt, glucose, glycine, or sucrose was added to 1% acetic acid, the solutes antagonized the action of the acid against E. coli O157:H7.
34416957	1	9	theme	laboratory	293:302	arg1	broth					304:308	laboratory broth	293:308	laboratory broth	293:308	This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth.
34416957	1	10	theme	acetic	168:173	arg1	acid					184:187	acetic or malic acid	168:187	acetic or malic acid	168:187	This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth.
34416957	7	11	theme	lactic	1248:1253	arg1	treatments					1275:1284	acetic, lactic, and propionic acid treatments	1240:1284	acetic, lactic, and propionic acid treatments and that the salt or sucrose significantly affects cell MFA composition	1240:1356	From the results of this study, the addition of solutes and organic compounds may increase the tolerance of E. coli O157:H7 to acetic, lactic, and propionic acid treatments and that the salt or sucrose significantly affects cell MFA composition.
34416957	1	12	dep	solutes	201:207	arg1	salt					210:213	salt	210:213	salt	210:213	This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth.
34416957	1	12	dep	solutes	201:207	arg1	solutes					201:207	various solutes	193:207	various solutes (salt, glucose, glycine, or sucrose)	193:244	This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth.
34416957	1	12	dep	solutes	201:207	arg1	glycine					225:231	glycine	225:231	glycine	225:231	This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth.
34416957	1	12	dep	solutes	201:207	arg1	sucrose					237:243	sucrose	237:243	sucrose	237:243	This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth.
34416957	1	12	dep	solutes	201:207	arg1	glucose					216:222	glucose	216:222	glucose	216:222	This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth.
34416957	4	13	dep	E.	729:730	arg1	coli					732:735	coli	732:735	coli	732:735	Whereas, when 3% salt, glucose, glycine, or sucrose was added to 1% acetic acid, the solutes antagonized the action of the acid against E. coli O157:H7.
34416957	1	14	from	O157	282:285	arg1	broth					304:308	laboratory broth	293:308	laboratory broth	293:308	This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth.
34416957	1	15	theme	malic	178:182	arg1	acid					184:187	acetic or malic acid	168:187	acetic or malic acid	168:187	This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth.
34416957	7	16	theme	acetic	1240:1245	arg1	treatments					1275:1284	acetic, lactic, and propionic acid treatments	1240:1284	acetic, lactic, and propionic acid treatments and that the salt or sucrose significantly affects cell MFA composition	1240:1356	From the results of this study, the addition of solutes and organic compounds may increase the tolerance of E. coli O157:H7 to acetic, lactic, and propionic acid treatments and that the salt or sucrose significantly affects cell MFA composition.
34416957	6	17	theme	cells	975:979	arg1	composition					960:970	the membrane fatty acid (MFA) composition	930:970	the membrane fatty acid (MFA) composition of cells	930:979	From a result of evaluating the membrane fatty acid (MFA) composition of cells, salt or sucrose significantly increased levels of saturated fatty acids (SFAs) or SFAs and cyclopropane fatty acids, respectively.
34416957	2	18	theme	activity	454:461	arg1	values					463:468	different water activity values	438:468	different water activity values against E. coli O157:H7	438:492	Additionally, the effectiveness of combining organic acids and various concentrations of salt (0-18%) or sucrose (0-100%) with different water activity values against E. coli O157:H7 were evaluated.
34416957	7	19	theme	MFA	1342:1344	arg1	composition					1346:1356	cell MFA composition	1337:1356	cell MFA composition	1337:1356	From the results of this study, the addition of solutes and organic compounds may increase the tolerance of E. coli O157:H7 to acetic, lactic, and propionic acid treatments and that the salt or sucrose significantly affects cell MFA composition.
34416957	7	20	theme	O157	1229:1232	arg1	tolerance					1208:1216	the tolerance	1204:1216	the tolerance of E. coli O157:H7 to acetic, lactic, and propionic acid treatments and that the salt or sucrose significantly affects cell MFA composition	1204:1356	From the results of this study, the addition of solutes and organic compounds may increase the tolerance of E. coli O157:H7 to acetic, lactic, and propionic acid treatments and that the salt or sucrose significantly affects cell MFA composition.
34416957	7	21	theme	E.	1221:1222	arg1	O157					1229:1232	E. coli O157	1221:1232	E. coli O157:H7	1221:1235	From the results of this study, the addition of solutes and organic compounds may increase the tolerance of E. coli O157:H7 to acetic, lactic, and propionic acid treatments and that the salt or sucrose significantly affects cell MFA composition.
34416957	6	22	theme	fatty	1042:1046	arg1	acids					1048:1052	saturated fatty acids	1032:1052	saturated fatty acids (SFAs)	1032:1059	From a result of evaluating the membrane fatty acid (MFA) composition of cells, salt or sucrose significantly increased levels of saturated fatty acids (SFAs) or SFAs and cyclopropane fatty acids, respectively.
34416957	6	22	theme	fatty	1042:1046	arg1	SFAs					1055:1058	SFAs	1055:1058	SFAs	1055:1058	From a result of evaluating the membrane fatty acid (MFA) composition of cells, salt or sucrose significantly increased levels of saturated fatty acids (SFAs) or SFAs and cyclopropane fatty acids, respectively.
34416957	1	23	theme	Escherichia	265:275	arg1	O157					282:285	Escherichia coli O157	265:285	Escherichia coli O157:H7 in laboratory broth	265:308	This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth.
34416957	4	24	theme	3	607:607	arg1	%					608:608	%	608:608	%	608:608	Whereas, when 3% salt, glucose, glycine, or sucrose was added to 1% acetic acid, the solutes antagonized the action of the acid against E. coli O157:H7.
34416957	1	25	theme	O157	282:285	arg1	survival					253:260	the survival	249:260	the survival of Escherichia coli O157:H7 in laboratory broth	249:308	This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth.
34416957	2	26	theme	sucrose	416:422	arg1	acids					364:368	organic acids	356:368	organic acids	356:368	Additionally, the effectiveness of combining organic acids and various concentrations of salt (0-18%) or sucrose (0-100%) with different water activity values against E. coli O157:H7 were evaluated.
34416957	2	26	theme	sucrose	416:422	arg1	concentrations					382:395	various concentrations	374:395	various concentrations of salt (0-18%) or sucrose (0-100%)	374:431	Additionally, the effectiveness of combining organic acids and various concentrations of salt (0-18%) or sucrose (0-100%) with different water activity values against E. coli O157:H7 were evaluated.
34416957	1	27	from	broth	304:308	arg1	survival					253:260	the survival	249:260	the survival of Escherichia coli O157:H7 in laboratory broth	249:308	This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth.
34416957	6	28	theme	acids	1092:1096	arg1	levels					1022:1027	levels	1022:1027	levels of saturated fatty acids (SFAs) or SFAs and cyclopropane fatty acids	1022:1096	From a result of evaluating the membrane fatty acid (MFA) composition of cells, salt or sucrose significantly increased levels of saturated fatty acids (SFAs) or SFAs and cyclopropane fatty acids, respectively.
34416957	2	29	theme	organic	356:362	arg1	acids					364:368	organic acids	356:368	organic acids	356:368	Additionally, the effectiveness of combining organic acids and various concentrations of salt (0-18%) or sucrose (0-100%) with different water activity values against E. coli O157:H7 were evaluated.
34416957	6	30	theme	cyclopropane	1073:1084	arg1	acids					1092:1096	SFAs and cyclopropane fatty acids	1064:1096	acids	1092:1096	From a result of evaluating the membrane fatty acid (MFA) composition of cells, salt or sucrose significantly increased levels of saturated fatty acids (SFAs) or SFAs and cyclopropane fatty acids, respectively.
34416957	5	31	theme	E.	885:886	arg1	O157					893:896	E. coli O157	885:896	E. coli O157:H7	885:899	Acetic, lactic, or propionic acid combined with salt at either 7 or 9% or sucrose at 60, 80, or 100% resulted in the highest resistance of E. coli O157:H7.
34416957	7	32	theme	organic	1173:1179	arg1	compounds					1181:1189	organic compounds	1173:1189	organic compounds	1173:1189	From the results of this study, the addition of solutes and organic compounds may increase the tolerance of E. coli O157:H7 to acetic, lactic, and propionic acid treatments and that the salt or sucrose significantly affects cell MFA composition.
34416957	7	33	theme	propionic	1260:1268	arg1	treatments					1275:1284	acetic, lactic, and propionic acid treatments	1240:1284	acetic, lactic, and propionic acid treatments and that the salt or sucrose significantly affects cell MFA composition	1240:1356	From the results of this study, the addition of solutes and organic compounds may increase the tolerance of E. coli O157:H7 to acetic, lactic, and propionic acid treatments and that the salt or sucrose significantly affects cell MFA composition.
34416957	2	34	theme	salt	400:403	arg1	acids					364:368	organic acids	356:368	organic acids	356:368	Additionally, the effectiveness of combining organic acids and various concentrations of salt (0-18%) or sucrose (0-100%) with different water activity values against E. coli O157:H7 were evaluated.
34416957	2	34	theme	salt	400:403	arg1	concentrations					382:395	various concentrations	374:395	various concentrations of salt (0-18%) or sucrose (0-100%)	374:431	Additionally, the effectiveness of combining organic acids and various concentrations of salt (0-18%) or sucrose (0-100%) with different water activity values against E. coli O157:H7 were evaluated.
34416957	3	35	theme	1	527:527	arg1	%					528:528	%	528:528	%	528:528	For treatment of 1% malic acid, the addition of 3% salt showed synergistic effect.
34416957	6	36	theme	SFAs	1064:1067	arg1	acids					1092:1096	SFAs and cyclopropane fatty acids	1064:1096	acids	1092:1096	From a result of evaluating the membrane fatty acid (MFA) composition of cells, salt or sucrose significantly increased levels of saturated fatty acids (SFAs) or SFAs and cyclopropane fatty acids, respectively.
34416957	5	37	from	%	845:845	arg1	sucrose					820:826	sucrose	820:826	sucrose at 60, 80, or 100%	820:845	Acetic, lactic, or propionic acid combined with salt at either 7 or 9% or sucrose at 60, 80, or 100% resulted in the highest resistance of E. coli O157:H7.
34416957	5	37	from	%	845:845	arg1	salt					794:797	salt	794:797	salt at either 7 or 9%	794:815	Acetic, lactic, or propionic acid combined with salt at either 7 or 9% or sucrose at 60, 80, or 100% resulted in the highest resistance of E. coli O157:H7.
34416957	7	38	theme	solutes	1161:1167	arg1	addition					1149:1156	the addition	1145:1156	the addition of solutes and organic compounds	1145:1189	From the results of this study, the addition of solutes and organic compounds may increase the tolerance of E. coli O157:H7 to acetic, lactic, and propionic acid treatments and that the salt or sucrose significantly affects cell MFA composition.
34416957	1	39	theme	acid	184:187	arg1	combinations					152:163	combinations	152:163	combinations of acetic or malic acid	152:187	This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth.
34416957	1	39	theme	acid	184:187	arg1	solutes					201:207	various solutes	193:207	various solutes (salt, glucose, glycine, or sucrose)	193:244	This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth.
34416957	1	39	theme	acid	184:187	arg1	glycine					225:231	glycine	225:231	glycine	225:231	This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth.
34416957	1	39	theme	acid	184:187	arg1	salt					210:213	salt	210:213	salt	210:213	This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth.
34416957	1	39	theme	acid	184:187	arg1	sucrose					237:243	sucrose	237:243	sucrose	237:243	This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth.
34416957	1	39	theme	acid	184:187	arg1	glucose					216:222	glucose	216:222	glucose	216:222	This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth.
34416957	3	40	theme	malic	530:534	arg1	acid					536:539	1% malic acid	527:539	1% malic acid	527:539	For treatment of 1% malic acid, the addition of 3% salt showed synergistic effect.
34416957	1	41	theme	various	193:199	arg1	salt					210:213	salt	210:213	salt	210:213	This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth.
34416957	1	41	theme	various	193:199	arg1	solutes					201:207	various solutes	193:207	various solutes (salt, glucose, glycine, or sucrose)	193:244	This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth.
34416957	1	41	theme	various	193:199	arg1	glycine					225:231	glycine	225:231	glycine	225:231	This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth.
34416957	1	41	theme	various	193:199	arg1	sucrose					237:243	sucrose	237:243	sucrose	237:243	This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth.
34416957	1	41	theme	various	193:199	arg1	glucose					216:222	glucose	216:222	glucose	216:222	This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth.
34416957	5	42	theme	propionic	765:773	arg1	acid					775:778	Acetic, lactic, or propionic acid	746:778	Acetic, lactic, or propionic acid combined with salt at either 7 or 9% or sucrose at 60, 80, or 100%	746:845	Acetic, lactic, or propionic acid combined with salt at either 7 or 9% or sucrose at 60, 80, or 100% resulted in the highest resistance of E. coli O157:H7.
34416957	0	43	from	treatment	82:90	arg1	media					106:110	laboratory media	95:110	laboratory media	95:110	Salt, glucose, glycine, and sucrose protect Escherichia coli O157:H7 against acid treatment in laboratory media.
34416957	3	44	theme	3	558:558	arg1	%					559:559	%	559:559	%	559:559	For treatment of 1% malic acid, the addition of 3% salt showed synergistic effect.
34416957	5	45	theme	O157	893:896	arg1	resistance					871:880	the highest resistance	859:880	the highest resistance of E. coli O157:H7	859:899	Acetic, lactic, or propionic acid combined with salt at either 7 or 9% or sucrose at 60, 80, or 100% resulted in the highest resistance of E. coli O157:H7.
34416957	4	46	theme	acetic	661:666	arg1	acid					668:671	1% acetic acid	658:671	1% acetic acid	658:671	Whereas, when 3% salt, glucose, glycine, or sucrose was added to 1% acetic acid, the solutes antagonized the action of the acid against E. coli O157:H7.
34416957	2	47	theme	E.	478:479	arg1	O157					486:489	E. coli O157	478:489	E. coli O157:H7	478:492	Additionally, the effectiveness of combining organic acids and various concentrations of salt (0-18%) or sucrose (0-100%) with different water activity values against E. coli O157:H7 were evaluated.
34416957	3	48	theme	synergistic	573:583	arg1	effect					585:590	synergistic effect	573:590	synergistic effect	573:590	For treatment of 1% malic acid, the addition of 3% salt showed synergistic effect.
34416957	6	49	theme	fatty	943:947	arg1	composition					960:970	the membrane fatty acid (MFA) composition	930:970	the membrane fatty acid (MFA) composition of cells	930:979	From a result of evaluating the membrane fatty acid (MFA) composition of cells, salt or sucrose significantly increased levels of saturated fatty acids (SFAs) or SFAs and cyclopropane fatty acids, respectively.
34416957	2	50	theme	various	374:380	arg1	concentrations					382:395	various concentrations	374:395	various concentrations of salt (0-18%) or sucrose (0-100%)	374:431	Additionally, the effectiveness of combining organic acids and various concentrations of salt (0-18%) or sucrose (0-100%) with different water activity values against E. coli O157:H7 were evaluated.
34416957	3	51	theme	salt	561:564	arg1	addition					546:553	the addition	542:553	the addition of 3% salt	542:564	For treatment of 1% malic acid, the addition of 3% salt showed synergistic effect.
34416957	4	52	theme	1	658:658	arg1	%					659:659	%	659:659	%	659:659	Whereas, when 3% salt, glucose, glycine, or sucrose was added to 1% acetic acid, the solutes antagonized the action of the acid against E. coli O157:H7.
34416957	7	53	dep	E.	1221:1222	arg1	coli					1224:1227	coli	1224:1227	coli	1224:1227	From the results of this study, the addition of solutes and organic compounds may increase the tolerance of E. coli O157:H7 to acetic, lactic, and propionic acid treatments and that the salt or sucrose significantly affects cell MFA composition.
34416957	2	54	dep	E.	478:479	arg1	coli					481:484	coli	481:484	coli	481:484	Additionally, the effectiveness of combining organic acids and various concentrations of salt (0-18%) or sucrose (0-100%) with different water activity values against E. coli O157:H7 were evaluated.
34416957	4	55	theme	acid	716:719	arg1	action					702:707	the action	698:707	the action of the acid against E. coli O157:H7	698:743	Whereas, when 3% salt, glucose, glycine, or sucrose was added to 1% acetic acid, the solutes antagonized the action of the acid against E. coli O157:H7.
34416957	7	56	dep	O157	1229:1232	arg1	H7					1234:1235	H7	1234:1235	E. coli O157:H7	1221:1235	From the results of this study, the addition of solutes and organic compounds may increase the tolerance of E. coli O157:H7 to acetic, lactic, and propionic acid treatments and that the salt or sucrose significantly affects cell MFA composition.
34416957	2	57	dep	O157	486:489	arg1	H7					491:492	H7	491:492	E. coli O157:H7	478:492	Additionally, the effectiveness of combining organic acids and various concentrations of salt (0-18%) or sucrose (0-100%) with different water activity values against E. coli O157:H7 were evaluated.
34416957	2	58	theme	water	448:452	arg1	values					463:468	different water activity values	438:468	different water activity values against E. coli O157:H7	438:492	Additionally, the effectiveness of combining organic acids and various concentrations of salt (0-18%) or sucrose (0-100%) with different water activity values against E. coli O157:H7 were evaluated.
34416957	5	59	dep	O157	893:896	arg1	H7					898:899	H7	898:899	E. coli O157:H7	885:899	Acetic, lactic, or propionic acid combined with salt at either 7 or 9% or sucrose at 60, 80, or 100% resulted in the highest resistance of E. coli O157:H7.
34416957	0	60	theme	Escherichia	44:54	arg1	O157					61:64	Escherichia coli O157	44:64	Escherichia coli O157:H7	44:67	Salt, glucose, glycine, and sucrose protect Escherichia coli O157:H7 against acid treatment in laboratory media.
34416957	5	61	dep	E.	885:886	arg1	coli					888:891	coli	888:891	coli	888:891	Acetic, lactic, or propionic acid combined with salt at either 7 or 9% or sucrose at 60, 80, or 100% resulted in the highest resistance of E. coli O157:H7.
34416957	7	62	dep	treatments	1275:1284	arg1	affects					1329:1335	affects	1329:1335	affects cell MFA composition	1329:1356	From the results of this study, the addition of solutes and organic compounds may increase the tolerance of E. coli O157:H7 to acetic, lactic, and propionic acid treatments and that the salt or sucrose significantly affects cell MFA composition.
34416957	6	63	theme	acids	1048:1052	arg1	levels					1022:1027	levels	1022:1027	levels of saturated fatty acids (SFAs) or SFAs and cyclopropane fatty acids	1022:1096	From a result of evaluating the membrane fatty acid (MFA) composition of cells, salt or sucrose significantly increased levels of saturated fatty acids (SFAs) or SFAs and cyclopropane fatty acids, respectively.
34416957	1	64	from	effects	141:147	arg1	survival					253:260	the survival	249:260	the survival of Escherichia coli O157:H7 in laboratory broth	249:308	This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth.
34416957	7	65	theme	cell	1337:1340	arg1	composition					1346:1356	cell MFA composition	1337:1356	cell MFA composition	1337:1356	From the results of this study, the addition of solutes and organic compounds may increase the tolerance of E. coli O157:H7 to acetic, lactic, and propionic acid treatments and that the salt or sucrose significantly affects cell MFA composition.
34416957	6	66	theme	saturated	1032:1040	arg1	acids					1048:1052	saturated fatty acids	1032:1052	saturated fatty acids (SFAs)	1032:1059	From a result of evaluating the membrane fatty acid (MFA) composition of cells, salt or sucrose significantly increased levels of saturated fatty acids (SFAs) or SFAs and cyclopropane fatty acids, respectively.
34416957	6	66	theme	saturated	1032:1040	arg1	SFAs					1055:1058	SFAs	1055:1058	SFAs	1055:1058	From a result of evaluating the membrane fatty acid (MFA) composition of cells, salt or sucrose significantly increased levels of saturated fatty acids (SFAs) or SFAs and cyclopropane fatty acids, respectively.
34416957	4	67	theme	E.	729:730	arg1	O157					737:740	E. coli O157	729:740	E. coli O157:H7	729:743	Whereas, when 3% salt, glucose, glycine, or sucrose was added to 1% acetic acid, the solutes antagonized the action of the acid against E. coli O157:H7.
34416957	0	68	theme	acid	77:80	arg1	treatment					82:90	acid treatment	77:90	acid treatment in laboratory media	77:110	Salt, glucose, glycine, and sucrose protect Escherichia coli O157:H7 against acid treatment in laboratory media.
34416957	5	69	theme	Acetic	746:751	arg1	acid					775:778	Acetic, lactic, or propionic acid	746:778	Acetic, lactic, or propionic acid combined with salt at either 7 or 9% or sucrose at 60, 80, or 100%	746:845	Acetic, lactic, or propionic acid combined with salt at either 7 or 9% or sucrose at 60, 80, or 100% resulted in the highest resistance of E. coli O157:H7.
34416957	4	70	theme	%	608:608	arg1	salt					610:613	3% salt	607:613	3% salt	607:613	Whereas, when 3% salt, glucose, glycine, or sucrose was added to 1% acetic acid, the solutes antagonized the action of the acid against E. coli O157:H7.
34416957	0	71	dep	O157	61:64	arg1	H7					66:67	H7	66:67	Escherichia coli O157:H7	44:67	Salt, glucose, glycine, and sucrose protect Escherichia coli O157:H7 against acid treatment in laboratory media.
34416957	1	72	dep	Escherichia	265:275	arg1	coli					277:280	coli	277:280	coli	277:280	This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth.
34416957	6	73	theme	fatty	1086:1090	arg1	acids					1092:1096	SFAs and cyclopropane fatty acids	1064:1096	acids	1092:1096	From a result of evaluating the membrane fatty acid (MFA) composition of cells, salt or sucrose significantly increased levels of saturated fatty acids (SFAs) or SFAs and cyclopropane fatty acids, respectively.
34416957	0	74	dep	Escherichia	44:54	arg1	coli					56:59	coli	56:59	coli	56:59	Salt, glucose, glycine, and sucrose protect Escherichia coli O157:H7 against acid treatment in laboratory media.
34416957	7	75	theme	compounds	1181:1189	arg1	addition					1149:1156	the addition	1145:1156	the addition of solutes and organic compounds	1145:1189	From the results of this study, the addition of solutes and organic compounds may increase the tolerance of E. coli O157:H7 to acetic, lactic, and propionic acid treatments and that the salt or sucrose significantly affects cell MFA composition.
34416957	7	76	theme	acid	1270:1273	arg1	treatments					1275:1284	acetic, lactic, and propionic acid treatments	1240:1284	acetic, lactic, and propionic acid treatments and that the salt or sucrose significantly affects cell MFA composition	1240:1356	From the results of this study, the addition of solutes and organic compounds may increase the tolerance of E. coli O157:H7 to acetic, lactic, and propionic acid treatments and that the salt or sucrose significantly affects cell MFA composition.
34416957	5	77	from	%	815:815	arg1	sucrose					820:826	sucrose	820:826	sucrose at 60, 80, or 100%	820:845	Acetic, lactic, or propionic acid combined with salt at either 7 or 9% or sucrose at 60, 80, or 100% resulted in the highest resistance of E. coli O157:H7.
34416957	5	77	from	%	815:815	arg1	salt					794:797	salt	794:797	salt at either 7 or 9%	794:815	Acetic, lactic, or propionic acid combined with salt at either 7 or 9% or sucrose at 60, 80, or 100% resulted in the highest resistance of E. coli O157:H7.
34416957	1	78	from	survival	253:260	arg1	broth					304:308	laboratory broth	293:308	laboratory broth	293:308	This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth.
34416957	0	79	theme	laboratory	95:104	arg1	media					106:110	laboratory media	95:110	laboratory media	95:110	Salt, glucose, glycine, and sucrose protect Escherichia coli O157:H7 against acid treatment in laboratory media.
34416957	1	80	dep	O157	282:285	arg1	H7					287:288	H7	287:288	Escherichia coli O157:H7 in laboratory broth	265:308	This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth.
34416957	3	81	theme	acid	536:539	arg1	treatment					514:522	treatment	514:522	treatment of 1% malic acid	514:539	For treatment of 1% malic acid, the addition of 3% salt showed synergistic effect.
34416957	5	82	theme	highest	863:869	arg1	resistance					871:880	the highest resistance	859:880	the highest resistance of E. coli O157:H7	859:899	Acetic, lactic, or propionic acid combined with salt at either 7 or 9% or sucrose at 60, 80, or 100% resulted in the highest resistance of E. coli O157:H7.
34416957	5	83	theme	lactic	754:759	arg1	acid					775:778	Acetic, lactic, or propionic acid	746:778	Acetic, lactic, or propionic acid combined with salt at either 7 or 9% or sucrose at 60, 80, or 100%	746:845	Acetic, lactic, or propionic acid combined with salt at either 7 or 9% or sucrose at 60, 80, or 100% resulted in the highest resistance of E. coli O157:H7.
34416957	3	84	theme	%	528:528	arg1	acid					536:539	1% malic acid	527:539	1% malic acid	527:539	For treatment of 1% malic acid, the addition of 3% salt showed synergistic effect.
34416957	1	85	theme	solutes	201:207	arg1	effects					141:147	the effects	137:147	the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth	137:308	This study investigated the effects of combinations of acetic or malic acid and various solutes (salt, glucose, glycine, or sucrose) on the survival of Escherichia coli O157:H7 in laboratory broth.
32853609	2	0	theme	dynamic	674:680	arg1	scattering					688:697	dynamic light scattering	674:697	dynamic light scattering	674:697	The physico-chemical characterization of chitosan, chitosan nanoparticles and Morinda citrifolia essential oils loaded chitosan nanoparticles combination was carried out by Fourier transform infrared spectroscopy, powder X-ray diffraction and dynamic light scattering coupled with zeta potential.
32853609	2	1	theme	powder	645:650	arg1	diffraction					658:668	powder X-ray diffraction	645:668	powder X-ray diffraction	645:668	The physico-chemical characterization of chitosan, chitosan nanoparticles and Morinda citrifolia essential oils loaded chitosan nanoparticles combination was carried out by Fourier transform infrared spectroscopy, powder X-ray diffraction and dynamic light scattering coupled with zeta potential.
32853609	3	2	theme	scanning	770:777	arg1	microscopy					788:797	scanning electron microscopy	770:797	scanning electron microscopy	770:797	The morphological observation obtained by scanning electron microscopy and transmission electron microscopy provided clear indication that the immobile chitosan polymer formed a coating onto the Morinda citrifolia essential oils surface.
32853609	7	3	theme	cancer	1665:1670	arg1	cells					1672:1676	A549 cancer cells	1660:1676	A549 cancer cells	1660:1676	Altogether, the present study suggests that these Morinda citrifolia essential oils loaded chitosan nanoparticles are valuable biomaterials owing to their ability to fight against A549 cancer cells.
32853609	4	4	theme	citrifolia	998:1007	arg1	oils					1019:1022	Morinda citrifolia essential oils	990:1022	Morinda citrifolia essential oils loaded chitosan nanoparticles against A549 cells	990:1071	The cytotoxic effect of Morinda citrifolia essential oils loaded chitosan nanoparticles against A549 cells were investigated, resulting in 54% inhibition at 40 μg/ml-1.
32853609	2	5	dep	transform	612:620	arg1	infrared					622:629	infrared	622:629	transform infrared spectroscopy, powder X-ray diffraction and dynamic light scattering coupled with zeta potential	612:725	The physico-chemical characterization of chitosan, chitosan nanoparticles and Morinda citrifolia essential oils loaded chitosan nanoparticles combination was carried out by Fourier transform infrared spectroscopy, powder X-ray diffraction and dynamic light scattering coupled with zeta potential.
32853609	3	6	theme	transmission	803:814	arg1	microscopy					825:834	transmission electron microscopy	803:834	transmission electron microscopy	803:834	The morphological observation obtained by scanning electron microscopy and transmission electron microscopy provided clear indication that the immobile chitosan polymer formed a coating onto the Morinda citrifolia essential oils surface.
32853609	6	7	theme	essential	1405:1413	arg1	chitosan					1427:1434	chitosan	1427:1434	chitosan	1427:1434	The toxicity evaluation against human red blood cells suggested that the Morinda citrifolia essential oils loaded chitosan nanoparticles possess minimum cytotoxicity.
32853609	6	7	theme	essential	1405:1413	arg1	oils					1415:1418	the Morinda citrifolia essential oils	1382:1418	the Morinda citrifolia essential oils loaded chitosan nanoparticles	1382:1448	The toxicity evaluation against human red blood cells suggested that the Morinda citrifolia essential oils loaded chitosan nanoparticles possess minimum cytotoxicity.
32853609	6	7	theme	essential	1405:1413	arg1	nanoparticles					1436:1448	nanoparticles	1436:1448	nanoparticles	1436:1448	The toxicity evaluation against human red blood cells suggested that the Morinda citrifolia essential oils loaded chitosan nanoparticles possess minimum cytotoxicity.
32853609	1	8	contain	contain	276:282	arg1	composition					155:165	the chemical composition	142:165	the chemical composition of Morinda citrifolia essential oils	142:202	In the present study, the chemical composition of Morinda citrifolia essential oils was determined by gas chromatography-mass spectrometry and was found to contain several anti-cancer compounds including L-scopoletin, nordamnacanthal, β-morindone, α-copaene, 9-H-pyrido[3,4-b]indole, β-thujene and terpinolene.
32853609	1	8	contain	contain	276:282	arg2	compounds					304:312	several anti-cancer compounds	284:312	several anti-cancer compounds including L-scopoletin, nordamnacanthal, β-morindone, α-copaene, 9-H-pyrido[3,4-b]indole, β-thujene and terpinolene	284:428	In the present study, the chemical composition of Morinda citrifolia essential oils was determined by gas chromatography-mass spectrometry and was found to contain several anti-cancer compounds including L-scopoletin, nordamnacanthal, β-morindone, α-copaene, 9-H-pyrido[3,4-b]indole, β-thujene and terpinolene.
32853609	1	8	contain	contain	276:282	arg2	L-scopoletin					324:335	L-scopoletin	324:335	L-scopoletin	324:335	In the present study, the chemical composition of Morinda citrifolia essential oils was determined by gas chromatography-mass spectrometry and was found to contain several anti-cancer compounds including L-scopoletin, nordamnacanthal, β-morindone, α-copaene, 9-H-pyrido[3,4-b]indole, β-thujene and terpinolene.
32853609	4	9	theme	cytotoxic	970:978	arg1	effect					980:985	The cytotoxic effect	966:985	The cytotoxic effect of Morinda citrifolia essential oils loaded chitosan nanoparticles against A549 cells	966:1071	The cytotoxic effect of Morinda citrifolia essential oils loaded chitosan nanoparticles against A549 cells were investigated, resulting in 54% inhibition at 40 μg/ml-1.
32853609	6	10	theme	Morinda	1386:1392	arg1	chitosan					1427:1434	chitosan	1427:1434	chitosan	1427:1434	The toxicity evaluation against human red blood cells suggested that the Morinda citrifolia essential oils loaded chitosan nanoparticles possess minimum cytotoxicity.
32853609	6	10	theme	Morinda	1386:1392	arg1	oils					1415:1418	the Morinda citrifolia essential oils	1382:1418	the Morinda citrifolia essential oils loaded chitosan nanoparticles	1382:1448	The toxicity evaluation against human red blood cells suggested that the Morinda citrifolia essential oils loaded chitosan nanoparticles possess minimum cytotoxicity.
32853609	6	10	theme	Morinda	1386:1392	arg1	nanoparticles					1436:1448	nanoparticles	1436:1448	nanoparticles	1436:1448	The toxicity evaluation against human red blood cells suggested that the Morinda citrifolia essential oils loaded chitosan nanoparticles possess minimum cytotoxicity.
32853609	1	11	theme	citrifolia	178:187	arg1	oils					199:202	Morinda citrifolia essential oils	170:202	Morinda citrifolia essential oils	170:202	In the present study, the chemical composition of Morinda citrifolia essential oils was determined by gas chromatography-mass spectrometry and was found to contain several anti-cancer compounds including L-scopoletin, nordamnacanthal, β-morindone, α-copaene, 9-H-pyrido[3,4-b]indole, β-thujene and terpinolene.
32853609	7	12	theme	chitosan	1571:1578	arg1	nanoparticles					1580:1592	these Morinda citrifolia essential oils loaded chitosan nanoparticles	1524:1592	these Morinda citrifolia essential oils loaded chitosan nanoparticles	1524:1592	Altogether, the present study suggests that these Morinda citrifolia essential oils loaded chitosan nanoparticles are valuable biomaterials owing to their ability to fight against A549 cancer cells.
32853609	7	12	theme	chitosan	1571:1578	arg1	biomaterials					1607:1618	valuable biomaterials	1598:1618	valuable biomaterials owing to their ability to fight against A549 cancer cells	1598:1676	Altogether, the present study suggests that these Morinda citrifolia essential oils loaded chitosan nanoparticles are valuable biomaterials owing to their ability to fight against A549 cancer cells.
32853609	3	13	theme	clear	845:849	arg1	indication					851:860	clear indication that the immobile chitosan polymer formed a coating onto the Morinda citrifolia essential oils surface	845:963	clear indication that the immobile chitosan polymer formed a coating onto the Morinda citrifolia essential oils surface	845:963	The morphological observation obtained by scanning electron microscopy and transmission electron microscopy provided clear indication that the immobile chitosan polymer formed a coating onto the Morinda citrifolia essential oils surface.
32853609	1	14	theme	oils	199:202	arg1	composition					155:165	the chemical composition	142:165	the chemical composition of Morinda citrifolia essential oils	142:202	In the present study, the chemical composition of Morinda citrifolia essential oils was determined by gas chromatography-mass spectrometry and was found to contain several anti-cancer compounds including L-scopoletin, nordamnacanthal, β-morindone, α-copaene, 9-H-pyrido[3,4-b]indole, β-thujene and terpinolene.
32853609	7	15	dep	Morinda	1530:1536	arg1	oils					1559:1562	essential oils	1549:1562	Morinda citrifolia essential oils	1530:1562	Altogether, the present study suggests that these Morinda citrifolia essential oils loaded chitosan nanoparticles are valuable biomaterials owing to their ability to fight against A549 cancer cells.
32853609	7	15	dep	Morinda	1530:1536	arg1	citrifolia					1538:1547	Morinda citrifolia essential oils	1530:1562	Morinda citrifolia essential oils	1530:1562	Altogether, the present study suggests that these Morinda citrifolia essential oils loaded chitosan nanoparticles are valuable biomaterials owing to their ability to fight against A549 cancer cells.
32853609	4	16	theme	oils	1019:1022	arg1	effect					980:985	The cytotoxic effect	966:985	The cytotoxic effect of Morinda citrifolia essential oils loaded chitosan nanoparticles against A549 cells	966:1071	The cytotoxic effect of Morinda citrifolia essential oils loaded chitosan nanoparticles against A549 cells were investigated, resulting in 54% inhibition at 40 μg/ml-1.
32853609	3	17	theme	immobile	871:878	arg1	polymer					889:895	the immobile chitosan polymer	867:895	the immobile chitosan polymer	867:895	The morphological observation obtained by scanning electron microscopy and transmission electron microscopy provided clear indication that the immobile chitosan polymer formed a coating onto the Morinda citrifolia essential oils surface.
32853609	3	18	dep	Morinda	923:929	arg1	citrifolia					931:940	Morinda citrifolia essential oils	923:955	the Morinda citrifolia essential oils surface	919:963	The morphological observation obtained by scanning electron microscopy and transmission electron microscopy provided clear indication that the immobile chitosan polymer formed a coating onto the Morinda citrifolia essential oils surface.
32853609	3	18	dep	Morinda	923:929	arg1	oils					952:955	essential oils	942:955	the Morinda citrifolia essential oils surface	919:963	The morphological observation obtained by scanning electron microscopy and transmission electron microscopy provided clear indication that the immobile chitosan polymer formed a coating onto the Morinda citrifolia essential oils surface.
32853609	4	19	theme	chitosan	1031:1038	arg1	nanoparticles					1040:1052	chitosan nanoparticles	1031:1052	chitosan nanoparticles	1031:1052	The cytotoxic effect of Morinda citrifolia essential oils loaded chitosan nanoparticles against A549 cells were investigated, resulting in 54% inhibition at 40 μg/ml-1.
32853609	6	20	theme	blood	1355:1359	arg1	cells					1361:1365	human red blood cells	1345:1365	human red blood cells	1345:1365	The toxicity evaluation against human red blood cells suggested that the Morinda citrifolia essential oils loaded chitosan nanoparticles possess minimum cytotoxicity.
32853609	6	21	theme	minimum	1458:1464	arg1	cytotoxicity					1466:1477	minimum cytotoxicity	1458:1477	minimum cytotoxicity	1458:1477	The toxicity evaluation against human red blood cells suggested that the Morinda citrifolia essential oils loaded chitosan nanoparticles possess minimum cytotoxicity.
32853609	5	22	theme	nucleus	1190:1196	arg1	damages					1198:1204	nucleus damages	1190:1204	nucleus damages	1190:1204	Information about in vitro morphological modification, nucleus damages, ROS generation and cell cycle arrest was obtained by fluorescence microscopy and flow cytometer analysis.
32853609	1	23	theme	present	127:133	arg1	study					135:139	the present study	123:139	the present study	123:139	In the present study, the chemical composition of Morinda citrifolia essential oils was determined by gas chromatography-mass spectrometry and was found to contain several anti-cancer compounds including L-scopoletin, nordamnacanthal, β-morindone, α-copaene, 9-H-pyrido[3,4-b]indole, β-thujene and terpinolene.
32853609	4	24	theme	54	1105:1106	arg1	%					1107:1107	%	1107:1107	%	1107:1107	The cytotoxic effect of Morinda citrifolia essential oils loaded chitosan nanoparticles against A549 cells were investigated, resulting in 54% inhibition at 40 μg/ml-1.
32853609	6	25	theme	human	1345:1349	arg1	cells					1361:1365	human red blood cells	1345:1365	human red blood cells	1345:1365	The toxicity evaluation against human red blood cells suggested that the Morinda citrifolia essential oils loaded chitosan nanoparticles possess minimum cytotoxicity.
32853609	0	26	theme	essential	68:76	arg1	oil					78:80	Morinda citrifolia essential oil	49:80	Morinda citrifolia essential oil	49:80	Enhanced anti-cancer activity of chitosan loaded Morinda citrifolia essential oil against A549 human lung cancer cells.
32853609	3	27	theme	essential	942:950	arg1	oils					952:955	essential oils	942:955	the Morinda citrifolia essential oils surface	919:963	The morphological observation obtained by scanning electron microscopy and transmission electron microscopy provided clear indication that the immobile chitosan polymer formed a coating onto the Morinda citrifolia essential oils surface.
32853609	5	28	theme	fluorescence	1260:1271	arg1	microscopy					1273:1282	fluorescence microscopy	1260:1282	fluorescence microscopy	1260:1282	Information about in vitro morphological modification, nucleus damages, ROS generation and cell cycle arrest was obtained by fluorescence microscopy and flow cytometer analysis.
32853609	1	29	theme	chemical	146:153	arg1	composition					155:165	the chemical composition	142:165	the chemical composition of Morinda citrifolia essential oils	142:202	In the present study, the chemical composition of Morinda citrifolia essential oils was determined by gas chromatography-mass spectrometry and was found to contain several anti-cancer compounds including L-scopoletin, nordamnacanthal, β-morindone, α-copaene, 9-H-pyrido[3,4-b]indole, β-thujene and terpinolene.
32853609	3	30	theme	Morinda	923:929	arg1	surface					957:963	the Morinda citrifolia essential oils surface	919:963	the Morinda citrifolia essential oils surface	919:963	The morphological observation obtained by scanning electron microscopy and transmission electron microscopy provided clear indication that the immobile chitosan polymer formed a coating onto the Morinda citrifolia essential oils surface.
32853609	0	31	theme	human	95:99	arg1	cells					113:117	A549 human lung cancer cells	90:117	A549 human lung cancer cells	90:117	Enhanced anti-cancer activity of chitosan loaded Morinda citrifolia essential oil against A549 human lung cancer cells.
32853609	5	32	theme	cell	1226:1229	arg1	arrest					1237:1242	cell cycle arrest	1226:1242	cell cycle arrest	1226:1242	Information about in vitro morphological modification, nucleus damages, ROS generation and cell cycle arrest was obtained by fluorescence microscopy and flow cytometer analysis.
32853609	1	33	dep	nordamnacanthal	338:352	arg1	α-copaene					368:376	α-copaene	368:376	α-copaene	368:376	In the present study, the chemical composition of Morinda citrifolia essential oils was determined by gas chromatography-mass spectrometry and was found to contain several anti-cancer compounds including L-scopoletin, nordamnacanthal, β-morindone, α-copaene, 9-H-pyrido[3,4-b]indole, β-thujene and terpinolene.
32853609	1	33	dep	nordamnacanthal	338:352	arg1	β-morindone					355:365	β-morindone	355:365	β-morindone	355:365	In the present study, the chemical composition of Morinda citrifolia essential oils was determined by gas chromatography-mass spectrometry and was found to contain several anti-cancer compounds including L-scopoletin, nordamnacanthal, β-morindone, α-copaene, 9-H-pyrido[3,4-b]indole, β-thujene and terpinolene.
32853609	1	33	dep	nordamnacanthal	338:352	arg1	indole					396:401	indole	396:401	indole	396:401	In the present study, the chemical composition of Morinda citrifolia essential oils was determined by gas chromatography-mass spectrometry and was found to contain several anti-cancer compounds including L-scopoletin, nordamnacanthal, β-morindone, α-copaene, 9-H-pyrido[3,4-b]indole, β-thujene and terpinolene.
32853609	2	34	theme	zeta	712:715	arg1	potential					717:725	zeta potential	712:725	zeta potential	712:725	The physico-chemical characterization of chitosan, chitosan nanoparticles and Morinda citrifolia essential oils loaded chitosan nanoparticles combination was carried out by Fourier transform infrared spectroscopy, powder X-ray diffraction and dynamic light scattering coupled with zeta potential.
32853609	0	35	theme	cancer	106:111	arg1	cells					113:117	A549 human lung cancer cells	90:117	A549 human lung cancer cells	90:117	Enhanced anti-cancer activity of chitosan loaded Morinda citrifolia essential oil against A549 human lung cancer cells.
32853609	2	36	theme	chitosan	482:489	arg1	chitosan					472:479	chitosan	472:479	chitosan	472:479	The physico-chemical characterization of chitosan, chitosan nanoparticles and Morinda citrifolia essential oils loaded chitosan nanoparticles combination was carried out by Fourier transform infrared spectroscopy, powder X-ray diffraction and dynamic light scattering coupled with zeta potential.
32853609	2	36	theme	chitosan	482:489	arg1	nanoparticles					491:503	chitosan nanoparticles	482:503	chitosan nanoparticles	482:503	The physico-chemical characterization of chitosan, chitosan nanoparticles and Morinda citrifolia essential oils loaded chitosan nanoparticles combination was carried out by Fourier transform infrared spectroscopy, powder X-ray diffraction and dynamic light scattering coupled with zeta potential.
32853609	0	37	theme	Enhanced	0:7	arg1	activity					21:28	Enhanced anti-cancer activity	0:28	Enhanced anti-cancer activity of chitosan	0:40	Enhanced anti-cancer activity of chitosan loaded Morinda citrifolia essential oil against A549 human lung cancer cells.
32853609	2	38	theme	chitosan	472:479	arg1	characterization					452:467	The physico-chemical characterization	431:467	The physico-chemical characterization of chitosan, chitosan nanoparticles and Morinda citrifolia essential oils loaded chitosan nanoparticles combination	431:583	The physico-chemical characterization of chitosan, chitosan nanoparticles and Morinda citrifolia essential oils loaded chitosan nanoparticles combination was carried out by Fourier transform infrared spectroscopy, powder X-ray diffraction and dynamic light scattering coupled with zeta potential.
32853609	1	39	theme	anti-cancer	292:302	arg1	L-scopoletin					324:335	L-scopoletin	324:335	L-scopoletin	324:335	In the present study, the chemical composition of Morinda citrifolia essential oils was determined by gas chromatography-mass spectrometry and was found to contain several anti-cancer compounds including L-scopoletin, nordamnacanthal, β-morindone, α-copaene, 9-H-pyrido[3,4-b]indole, β-thujene and terpinolene.
32853609	1	39	theme	anti-cancer	292:302	arg1	compounds					304:312	several anti-cancer compounds	284:312	several anti-cancer compounds including L-scopoletin, nordamnacanthal, β-morindone, α-copaene, 9-H-pyrido[3,4-b]indole, β-thujene and terpinolene	284:428	In the present study, the chemical composition of Morinda citrifolia essential oils was determined by gas chromatography-mass spectrometry and was found to contain several anti-cancer compounds including L-scopoletin, nordamnacanthal, β-morindone, α-copaene, 9-H-pyrido[3,4-b]indole, β-thujene and terpinolene.
32853609	2	40	theme	citrifolia	517:526	arg1	oils					538:541	Morinda citrifolia essential oils	509:541	Morinda citrifolia essential oils	509:541	The physico-chemical characterization of chitosan, chitosan nanoparticles and Morinda citrifolia essential oils loaded chitosan nanoparticles combination was carried out by Fourier transform infrared spectroscopy, powder X-ray diffraction and dynamic light scattering coupled with zeta potential.
32853609	2	40	theme	citrifolia	517:526	arg1	chitosan					472:479	chitosan	472:479	chitosan	472:479	The physico-chemical characterization of chitosan, chitosan nanoparticles and Morinda citrifolia essential oils loaded chitosan nanoparticles combination was carried out by Fourier transform infrared spectroscopy, powder X-ray diffraction and dynamic light scattering coupled with zeta potential.
32853609	0	41	theme	chitosan	33:40	arg1	activity					21:28	Enhanced anti-cancer activity	0:28	Enhanced anti-cancer activity of chitosan	0:40	Enhanced anti-cancer activity of chitosan loaded Morinda citrifolia essential oil against A549 human lung cancer cells.
32853609	5	42	theme	in	1153:1154	arg1	modification					1176:1187	in vitro morphological modification	1153:1187	in vitro morphological modification	1153:1187	Information about in vitro morphological modification, nucleus damages, ROS generation and cell cycle arrest was obtained by fluorescence microscopy and flow cytometer analysis.
32853609	0	43	theme	Morinda	49:55	arg1	oil					78:80	Morinda citrifolia essential oil	49:80	Morinda citrifolia essential oil	49:80	Enhanced anti-cancer activity of chitosan loaded Morinda citrifolia essential oil against A549 human lung cancer cells.
32853609	5	44	theme	cytometer	1293:1301	arg1	analysis					1303:1310	flow cytometer analysis	1288:1310	flow cytometer analysis	1288:1310	Information about in vitro morphological modification, nucleus damages, ROS generation and cell cycle arrest was obtained by fluorescence microscopy and flow cytometer analysis.
32853609	5	45	theme	morphological	1162:1174	arg1	modification					1176:1187	in vitro morphological modification	1153:1187	in vitro morphological modification	1153:1187	Information about in vitro morphological modification, nucleus damages, ROS generation and cell cycle arrest was obtained by fluorescence microscopy and flow cytometer analysis.
32853609	1	46	theme	chromatography-mass	226:244	arg1	spectrometry					246:257	gas chromatography-mass spectrometry	222:257	gas chromatography-mass spectrometry	222:257	In the present study, the chemical composition of Morinda citrifolia essential oils was determined by gas chromatography-mass spectrometry and was found to contain several anti-cancer compounds including L-scopoletin, nordamnacanthal, β-morindone, α-copaene, 9-H-pyrido[3,4-b]indole, β-thujene and terpinolene.
32853609	7	47	theme	A549	1660:1663	arg1	cells					1672:1676	A549 cancer cells	1660:1676	A549 cancer cells	1660:1676	Altogether, the present study suggests that these Morinda citrifolia essential oils loaded chitosan nanoparticles are valuable biomaterials owing to their ability to fight against A549 cancer cells.
32853609	3	48	theme	electron	779:786	arg1	microscopy					788:797	scanning electron microscopy	770:797	scanning electron microscopy	770:797	The morphological observation obtained by scanning electron microscopy and transmission electron microscopy provided clear indication that the immobile chitosan polymer formed a coating onto the Morinda citrifolia essential oils surface.
32853609	4	49	theme	%	1107:1107	arg1	inhibition					1109:1118	54% inhibition	1105:1118	54% inhibition at 40 μg/ml-1	1105:1132	The cytotoxic effect of Morinda citrifolia essential oils loaded chitosan nanoparticles against A549 cells were investigated, resulting in 54% inhibition at 40 μg/ml-1.
32853609	6	50	dep	loaded	1420:1425	arg1	chitosan					1427:1434	chitosan	1427:1434	chitosan	1427:1434	The toxicity evaluation against human red blood cells suggested that the Morinda citrifolia essential oils loaded chitosan nanoparticles possess minimum cytotoxicity.
32853609	6	50	dep	loaded	1420:1425	arg1	oils					1415:1418	the Morinda citrifolia essential oils	1382:1418	the Morinda citrifolia essential oils loaded chitosan nanoparticles	1382:1448	The toxicity evaluation against human red blood cells suggested that the Morinda citrifolia essential oils loaded chitosan nanoparticles possess minimum cytotoxicity.
32853609	6	50	dep	loaded	1420:1425	arg1	nanoparticles					1436:1448	nanoparticles	1436:1448	nanoparticles	1436:1448	The toxicity evaluation against human red blood cells suggested that the Morinda citrifolia essential oils loaded chitosan nanoparticles possess minimum cytotoxicity.
32853609	2	51	theme	X-ray	652:656	arg1	diffraction					658:668	powder X-ray diffraction	645:668	powder X-ray diffraction	645:668	The physico-chemical characterization of chitosan, chitosan nanoparticles and Morinda citrifolia essential oils loaded chitosan nanoparticles combination was carried out by Fourier transform infrared spectroscopy, powder X-ray diffraction and dynamic light scattering coupled with zeta potential.
32853609	6	52	theme	toxicity	1317:1324	arg1	evaluation					1326:1335	The toxicity evaluation	1313:1335	The toxicity evaluation against human red blood cells	1313:1365	The toxicity evaluation against human red blood cells suggested that the Morinda citrifolia essential oils loaded chitosan nanoparticles possess minimum cytotoxicity.
32853609	2	53	dep	Fourier	604:610	arg1	transform					612:620	transform	612:620	transform infrared spectroscopy, powder X-ray diffraction and dynamic light scattering coupled with zeta potential	612:725	The physico-chemical characterization of chitosan, chitosan nanoparticles and Morinda citrifolia essential oils loaded chitosan nanoparticles combination was carried out by Fourier transform infrared spectroscopy, powder X-ray diffraction and dynamic light scattering coupled with zeta potential.
32853609	4	54	from	40 μg/ml-1	1123:1132	arg1	inhibition					1109:1118	54% inhibition	1105:1118	54% inhibition at 40 μg/ml-1	1105:1132	The cytotoxic effect of Morinda citrifolia essential oils loaded chitosan nanoparticles against A549 cells were investigated, resulting in 54% inhibition at 40 μg/ml-1.
32853609	3	55	theme	electron	816:823	arg1	microscopy					825:834	transmission electron microscopy	803:834	transmission electron microscopy	803:834	The morphological observation obtained by scanning electron microscopy and transmission electron microscopy provided clear indication that the immobile chitosan polymer formed a coating onto the Morinda citrifolia essential oils surface.
32853609	6	56	theme	citrifolia	1394:1403	arg1	chitosan					1427:1434	chitosan	1427:1434	chitosan	1427:1434	The toxicity evaluation against human red blood cells suggested that the Morinda citrifolia essential oils loaded chitosan nanoparticles possess minimum cytotoxicity.
32853609	6	56	theme	citrifolia	1394:1403	arg1	oils					1415:1418	the Morinda citrifolia essential oils	1382:1418	the Morinda citrifolia essential oils loaded chitosan nanoparticles	1382:1448	The toxicity evaluation against human red blood cells suggested that the Morinda citrifolia essential oils loaded chitosan nanoparticles possess minimum cytotoxicity.
32853609	6	56	theme	citrifolia	1394:1403	arg1	nanoparticles					1436:1448	nanoparticles	1436:1448	nanoparticles	1436:1448	The toxicity evaluation against human red blood cells suggested that the Morinda citrifolia essential oils loaded chitosan nanoparticles possess minimum cytotoxicity.
32853609	1	57	theme	Morinda	170:176	arg1	oils					199:202	Morinda citrifolia essential oils	170:202	Morinda citrifolia essential oils	170:202	In the present study, the chemical composition of Morinda citrifolia essential oils was determined by gas chromatography-mass spectrometry and was found to contain several anti-cancer compounds including L-scopoletin, nordamnacanthal, β-morindone, α-copaene, 9-H-pyrido[3,4-b]indole, β-thujene and terpinolene.
32853609	2	58	theme	light	682:686	arg1	scattering					688:697	dynamic light scattering	674:697	dynamic light scattering	674:697	The physico-chemical characterization of chitosan, chitosan nanoparticles and Morinda citrifolia essential oils loaded chitosan nanoparticles combination was carried out by Fourier transform infrared spectroscopy, powder X-ray diffraction and dynamic light scattering coupled with zeta potential.
32853609	7	59	theme	valuable	1598:1605	arg1	nanoparticles					1580:1592	these Morinda citrifolia essential oils loaded chitosan nanoparticles	1524:1592	these Morinda citrifolia essential oils loaded chitosan nanoparticles	1524:1592	Altogether, the present study suggests that these Morinda citrifolia essential oils loaded chitosan nanoparticles are valuable biomaterials owing to their ability to fight against A549 cancer cells.
32853609	7	59	theme	valuable	1598:1605	arg1	biomaterials					1607:1618	valuable biomaterials	1598:1618	valuable biomaterials owing to their ability to fight against A549 cancer cells	1598:1676	Altogether, the present study suggests that these Morinda citrifolia essential oils loaded chitosan nanoparticles are valuable biomaterials owing to their ability to fight against A549 cancer cells.
32853609	5	60	dep	in	1153:1154	arg1	vitro					1156:1160	vitro	1156:1160	vitro	1156:1160	Information about in vitro morphological modification, nucleus damages, ROS generation and cell cycle arrest was obtained by fluorescence microscopy and flow cytometer analysis.
32853609	1	61	theme	essential	189:197	arg1	oils					199:202	Morinda citrifolia essential oils	170:202	Morinda citrifolia essential oils	170:202	In the present study, the chemical composition of Morinda citrifolia essential oils was determined by gas chromatography-mass spectrometry and was found to contain several anti-cancer compounds including L-scopoletin, nordamnacanthal, β-morindone, α-copaene, 9-H-pyrido[3,4-b]indole, β-thujene and terpinolene.
32853609	5	62	theme	ROS	1207:1209	arg1	generation					1211:1220	ROS generation	1207:1220	ROS generation	1207:1220	Information about in vitro morphological modification, nucleus damages, ROS generation and cell cycle arrest was obtained by fluorescence microscopy and flow cytometer analysis.
32853609	7	63	dep	loaded	1564:1569	arg1	Morinda					1530:1536	Morinda	1530:1536	Morinda	1530:1536	Altogether, the present study suggests that these Morinda citrifolia essential oils loaded chitosan nanoparticles are valuable biomaterials owing to their ability to fight against A549 cancer cells.
32853609	4	64	theme	A549	1062:1065	arg1	cells					1067:1071	A549 cells	1062:1071	A549 cells	1062:1071	The cytotoxic effect of Morinda citrifolia essential oils loaded chitosan nanoparticles against A549 cells were investigated, resulting in 54% inhibition at 40 μg/ml-1.
32853609	7	65	theme	loaded	1564:1569	arg1	nanoparticles					1580:1592	these Morinda citrifolia essential oils loaded chitosan nanoparticles	1524:1592	these Morinda citrifolia essential oils loaded chitosan nanoparticles	1524:1592	Altogether, the present study suggests that these Morinda citrifolia essential oils loaded chitosan nanoparticles are valuable biomaterials owing to their ability to fight against A549 cancer cells.
32853609	7	65	theme	loaded	1564:1569	arg1	biomaterials					1607:1618	valuable biomaterials	1598:1618	valuable biomaterials owing to their ability to fight against A549 cancer cells	1598:1676	Altogether, the present study suggests that these Morinda citrifolia essential oils loaded chitosan nanoparticles are valuable biomaterials owing to their ability to fight against A549 cancer cells.
32853609	4	66	theme	essential	1009:1017	arg1	oils					1019:1022	Morinda citrifolia essential oils	990:1022	Morinda citrifolia essential oils loaded chitosan nanoparticles against A549 cells	990:1071	The cytotoxic effect of Morinda citrifolia essential oils loaded chitosan nanoparticles against A549 cells were investigated, resulting in 54% inhibition at 40 μg/ml-1.
32853609	7	67	theme	essential	1549:1557	arg1	oils					1559:1562	essential oils	1549:1562	Morinda citrifolia essential oils	1530:1562	Altogether, the present study suggests that these Morinda citrifolia essential oils loaded chitosan nanoparticles are valuable biomaterials owing to their ability to fight against A549 cancer cells.
32853609	3	68	theme	chitosan	880:887	arg1	polymer					889:895	the immobile chitosan polymer	867:895	the immobile chitosan polymer	867:895	The morphological observation obtained by scanning electron microscopy and transmission electron microscopy provided clear indication that the immobile chitosan polymer formed a coating onto the Morinda citrifolia essential oils surface.
32853609	6	69	theme	red	1351:1353	arg1	cells					1361:1365	human red blood cells	1345:1365	human red blood cells	1345:1365	The toxicity evaluation against human red blood cells suggested that the Morinda citrifolia essential oils loaded chitosan nanoparticles possess minimum cytotoxicity.
32853609	4	70	theme	Morinda	990:996	arg1	oils					1019:1022	Morinda citrifolia essential oils	990:1022	Morinda citrifolia essential oils loaded chitosan nanoparticles against A549 cells	990:1071	The cytotoxic effect of Morinda citrifolia essential oils loaded chitosan nanoparticles against A549 cells were investigated, resulting in 54% inhibition at 40 μg/ml-1.
32853609	0	71	theme	A549	90:93	arg1	cells					113:117	A549 human lung cancer cells	90:117	A549 human lung cancer cells	90:117	Enhanced anti-cancer activity of chitosan loaded Morinda citrifolia essential oil against A549 human lung cancer cells.
32853609	2	72	theme	essential	528:536	arg1	oils					538:541	Morinda citrifolia essential oils	509:541	Morinda citrifolia essential oils	509:541	The physico-chemical characterization of chitosan, chitosan nanoparticles and Morinda citrifolia essential oils loaded chitosan nanoparticles combination was carried out by Fourier transform infrared spectroscopy, powder X-ray diffraction and dynamic light scattering coupled with zeta potential.
32853609	2	72	theme	essential	528:536	arg1	chitosan					472:479	chitosan	472:479	chitosan	472:479	The physico-chemical characterization of chitosan, chitosan nanoparticles and Morinda citrifolia essential oils loaded chitosan nanoparticles combination was carried out by Fourier transform infrared spectroscopy, powder X-ray diffraction and dynamic light scattering coupled with zeta potential.
32853609	0	73	theme	lung	101:104	arg1	cells					113:117	A549 human lung cancer cells	90:117	A549 human lung cancer cells	90:117	Enhanced anti-cancer activity of chitosan loaded Morinda citrifolia essential oil against A549 human lung cancer cells.
32853609	5	74	theme	cycle	1231:1235	arg1	arrest					1237:1242	cell cycle arrest	1226:1242	cell cycle arrest	1226:1242	Information about in vitro morphological modification, nucleus damages, ROS generation and cell cycle arrest was obtained by fluorescence microscopy and flow cytometer analysis.
32853609	7	75	theme	present	1496:1502	arg1	study					1504:1508	the present study	1492:1508	the present study	1492:1508	Altogether, the present study suggests that these Morinda citrifolia essential oils loaded chitosan nanoparticles are valuable biomaterials owing to their ability to fight against A549 cancer cells.
32853609	0	76	theme	anti-cancer	9:19	arg1	activity					21:28	Enhanced anti-cancer activity	0:28	Enhanced anti-cancer activity of chitosan	0:40	Enhanced anti-cancer activity of chitosan loaded Morinda citrifolia essential oil against A549 human lung cancer cells.
32853609	6	77	contain	possess	1450:1456	arg1	chitosan					1427:1434	chitosan	1427:1434	chitosan	1427:1434	The toxicity evaluation against human red blood cells suggested that the Morinda citrifolia essential oils loaded chitosan nanoparticles possess minimum cytotoxicity.
32853609	6	77	contain	possess	1450:1456	arg1	oils					1415:1418	the Morinda citrifolia essential oils	1382:1418	the Morinda citrifolia essential oils loaded chitosan nanoparticles	1382:1448	The toxicity evaluation against human red blood cells suggested that the Morinda citrifolia essential oils loaded chitosan nanoparticles possess minimum cytotoxicity.
32853609	6	77	contain	possess	1450:1456	arg1	nanoparticles					1436:1448	nanoparticles	1436:1448	nanoparticles	1436:1448	The toxicity evaluation against human red blood cells suggested that the Morinda citrifolia essential oils loaded chitosan nanoparticles possess minimum cytotoxicity.
32853609	6	77	contain	possess	1450:1456	arg2	cytotoxicity					1466:1477	minimum cytotoxicity	1458:1477	minimum cytotoxicity	1458:1477	The toxicity evaluation against human red blood cells suggested that the Morinda citrifolia essential oils loaded chitosan nanoparticles possess minimum cytotoxicity.
32853609	1	78	theme	several	284:290	arg1	L-scopoletin					324:335	L-scopoletin	324:335	L-scopoletin	324:335	In the present study, the chemical composition of Morinda citrifolia essential oils was determined by gas chromatography-mass spectrometry and was found to contain several anti-cancer compounds including L-scopoletin, nordamnacanthal, β-morindone, α-copaene, 9-H-pyrido[3,4-b]indole, β-thujene and terpinolene.
32853609	1	78	theme	several	284:290	arg1	compounds					304:312	several anti-cancer compounds	284:312	several anti-cancer compounds including L-scopoletin, nordamnacanthal, β-morindone, α-copaene, 9-H-pyrido[3,4-b]indole, β-thujene and terpinolene	284:428	In the present study, the chemical composition of Morinda citrifolia essential oils was determined by gas chromatography-mass spectrometry and was found to contain several anti-cancer compounds including L-scopoletin, nordamnacanthal, β-morindone, α-copaene, 9-H-pyrido[3,4-b]indole, β-thujene and terpinolene.
32853609	2	79	theme	physico-chemical	435:450	arg1	characterization					452:467	The physico-chemical characterization	431:467	The physico-chemical characterization of chitosan, chitosan nanoparticles and Morinda citrifolia essential oils loaded chitosan nanoparticles combination	431:583	The physico-chemical characterization of chitosan, chitosan nanoparticles and Morinda citrifolia essential oils loaded chitosan nanoparticles combination was carried out by Fourier transform infrared spectroscopy, powder X-ray diffraction and dynamic light scattering coupled with zeta potential.
32853609	2	80	theme	Morinda	509:515	arg1	oils					538:541	Morinda citrifolia essential oils	509:541	Morinda citrifolia essential oils	509:541	The physico-chemical characterization of chitosan, chitosan nanoparticles and Morinda citrifolia essential oils loaded chitosan nanoparticles combination was carried out by Fourier transform infrared spectroscopy, powder X-ray diffraction and dynamic light scattering coupled with zeta potential.
32853609	2	80	theme	Morinda	509:515	arg1	chitosan					472:479	chitosan	472:479	chitosan	472:479	The physico-chemical characterization of chitosan, chitosan nanoparticles and Morinda citrifolia essential oils loaded chitosan nanoparticles combination was carried out by Fourier transform infrared spectroscopy, powder X-ray diffraction and dynamic light scattering coupled with zeta potential.
32853609	4	81	dep	nanoparticles	1040:1052	arg1	cells					1067:1071	A549 cells	1062:1071	A549 cells	1062:1071	The cytotoxic effect of Morinda citrifolia essential oils loaded chitosan nanoparticles against A549 cells were investigated, resulting in 54% inhibition at 40 μg/ml-1.
32853609	0	82	theme	citrifolia	57:66	arg1	oil					78:80	Morinda citrifolia essential oil	49:80	Morinda citrifolia essential oil	49:80	Enhanced anti-cancer activity of chitosan loaded Morinda citrifolia essential oil against A549 human lung cancer cells.
32853609	5	83	theme	flow	1288:1291	arg1	analysis					1303:1310	flow cytometer analysis	1288:1310	flow cytometer analysis	1288:1310	Information about in vitro morphological modification, nucleus damages, ROS generation and cell cycle arrest was obtained by fluorescence microscopy and flow cytometer analysis.
32853609	1	84	theme	nordamnacanthal	338:352	arg1	L-scopoletin					324:335	L-scopoletin	324:335	L-scopoletin	324:335	In the present study, the chemical composition of Morinda citrifolia essential oils was determined by gas chromatography-mass spectrometry and was found to contain several anti-cancer compounds including L-scopoletin, nordamnacanthal, β-morindone, α-copaene, 9-H-pyrido[3,4-b]indole, β-thujene and terpinolene.
32853609	1	84	theme	nordamnacanthal	338:352	arg1	terpinolene					418:428	terpinolene	418:428	terpinolene	418:428	In the present study, the chemical composition of Morinda citrifolia essential oils was determined by gas chromatography-mass spectrometry and was found to contain several anti-cancer compounds including L-scopoletin, nordamnacanthal, β-morindone, α-copaene, 9-H-pyrido[3,4-b]indole, β-thujene and terpinolene.
32853609	1	84	theme	nordamnacanthal	338:352	arg1	β-thujene					404:412	β-thujene	404:412	β-thujene	404:412	In the present study, the chemical composition of Morinda citrifolia essential oils was determined by gas chromatography-mass spectrometry and was found to contain several anti-cancer compounds including L-scopoletin, nordamnacanthal, β-morindone, α-copaene, 9-H-pyrido[3,4-b]indole, β-thujene and terpinolene.
32853609	3	85	theme	morphological	732:744	arg1	observation					746:756	The morphological observation	728:756	The morphological observation obtained by scanning electron microscopy and transmission electron microscopy	728:834	The morphological observation obtained by scanning electron microscopy and transmission electron microscopy provided clear indication that the immobile chitosan polymer formed a coating onto the Morinda citrifolia essential oils surface.
32853609	1	86	theme	gas	222:224	arg1	spectrometry					246:257	gas chromatography-mass spectrometry	222:257	gas chromatography-mass spectrometry	222:257	In the present study, the chemical composition of Morinda citrifolia essential oils was determined by gas chromatography-mass spectrometry and was found to contain several anti-cancer compounds including L-scopoletin, nordamnacanthal, β-morindone, α-copaene, 9-H-pyrido[3,4-b]indole, β-thujene and terpinolene.
34793569	2	0	theme	dietary	455:461	arg1	chitosan					463:470	dietary chitosan	455:470	dietary chitosan	455:470	Three test diets containing three different concentrations (1, 2, and 3 g kg-1 feed) of dietary chitosan were formulated.
34793569	4	1	theme	acid	823:826	arg1	abundance					838:846	lactic acid bacterium abundance	816:846	lactic acid bacterium abundance	816:846	Comparing the effects of the dietary chitosan-containing diets with those of the control diet, we found that dietary chitosan significantly improved the muscle growth, nutrient and mineral contents, hematological parameters, lactic acid bacterium abundance, and digestive enzyme activities of B. gonionotus.
34793569	7	2	theme	kidney	1446:1451	arg1	morphology					1428:1437	the morphology	1424:1437	the morphology of the kidney compared to those with the basal diet	1424:1489	Quantitative study revealed that dietary chitosan significantly enhanced the length of intestinal villi, and qualitative study showed that dietary chitosan considerably reduced the fat content in the liver and improved the morphology of the kidney compared to those with the basal diet.
34793569	8	3	theme	kg-1	1586:1589	arg1	feed					1591:1594	1 g kg-1 feed	1582:1594	1 g kg-1 feed	1582:1594	Taken together, our results suggest that the application of dietary chitosan at a dose of 1 g kg-1 feed produced the highest benefit to treated B. gonionotus, indicating its potential for safe use in aquaculture.
34793569	1	4	theme	juvenile	335:342	arg1	gonionotus					355:364	juvenile Barbonymus gonionotus	335:364	juvenile Barbonymus gonionotus	335:364	In this study, we determined the effects of dietary chitosan on the growth, biochemical composition, gut microbiota, and hematological and histological parameters of juvenile Barbonymus gonionotus.
34793569	4	5	theme	lactic	816:821	arg1	abundance					838:846	lactic acid bacterium abundance	816:846	lactic acid bacterium abundance	816:846	Comparing the effects of the dietary chitosan-containing diets with those of the control diet, we found that dietary chitosan significantly improved the muscle growth, nutrient and mineral contents, hematological parameters, lactic acid bacterium abundance, and digestive enzyme activities of B. gonionotus.
34793569	8	6	theme	g	1584:1584	arg1	feed					1591:1594	1 g kg-1 feed	1582:1594	1 g kg-1 feed	1582:1594	Taken together, our results suggest that the application of dietary chitosan at a dose of 1 g kg-1 feed produced the highest benefit to treated B. gonionotus, indicating its potential for safe use in aquaculture.
34793569	1	7	theme	Barbonymus	344:353	arg1	gonionotus					355:364	juvenile Barbonymus gonionotus	335:364	juvenile Barbonymus gonionotus	335:364	In this study, we determined the effects of dietary chitosan on the growth, biochemical composition, gut microbiota, and hematological and histological parameters of juvenile Barbonymus gonionotus.
34793569	3	8	theme	dietary	510:516	arg1	chitosan					518:525	dietary chitosan	510:525	dietary chitosan	510:525	A basal diet without dietary chitosan was considered a control, and the fish were reared for 60 days.
34793569	1	9	theme	biochemical	245:255	arg1	composition					257:267	biochemical composition	245:267	biochemical composition	245:267	In this study, we determined the effects of dietary chitosan on the growth, biochemical composition, gut microbiota, and hematological and histological parameters of juvenile Barbonymus gonionotus.
34793569	0	10	theme	hematological	79:91	arg1	parameters					93:102	hematological parameters	79:102	hematological parameters	79:102	Dietary chitosan promotes the growth, biochemical composition, gut microbiota, hematological parameters and internal organ morphology of juvenile Barbonymus gonionotus.
34793569	1	11	theme	gonionotus	355:364	arg1	microbiota					274:283	gut microbiota	270:283	gut microbiota	270:283	In this study, we determined the effects of dietary chitosan on the growth, biochemical composition, gut microbiota, and hematological and histological parameters of juvenile Barbonymus gonionotus.
34793569	1	11	theme	gonionotus	355:364	arg1	parameters					321:330	hematological and histological parameters	290:330	hematological and histological parameters	290:330	In this study, we determined the effects of dietary chitosan on the growth, biochemical composition, gut microbiota, and hematological and histological parameters of juvenile Barbonymus gonionotus.
34793569	1	11	theme	gonionotus	355:364	arg1	growth					237:242	growth	237:242	growth	237:242	In this study, we determined the effects of dietary chitosan on the growth, biochemical composition, gut microbiota, and hematological and histological parameters of juvenile Barbonymus gonionotus.
34793569	1	11	theme	gonionotus	355:364	arg1	composition					257:267	biochemical composition	245:267	biochemical composition	245:267	In this study, we determined the effects of dietary chitosan on the growth, biochemical composition, gut microbiota, and hematological and histological parameters of juvenile Barbonymus gonionotus.
34793569	4	12	theme	bacterium	828:836	arg1	abundance					838:846	lactic acid bacterium abundance	816:846	lactic acid bacterium abundance	816:846	Comparing the effects of the dietary chitosan-containing diets with those of the control diet, we found that dietary chitosan significantly improved the muscle growth, nutrient and mineral contents, hematological parameters, lactic acid bacterium abundance, and digestive enzyme activities of B. gonionotus.
34793569	8	13	theme	feed	1591:1594	arg1	dose					1574:1577	a dose	1572:1577	a dose of 1 g kg-1 feed	1572:1594	Taken together, our results suggest that the application of dietary chitosan at a dose of 1 g kg-1 feed produced the highest benefit to treated B. gonionotus, indicating its potential for safe use in aquaculture.
34793569	4	14	theme	dietary	700:706	arg1	chitosan					708:715	dietary chitosan	700:715	dietary chitosan	700:715	Comparing the effects of the dietary chitosan-containing diets with those of the control diet, we found that dietary chitosan significantly improved the muscle growth, nutrient and mineral contents, hematological parameters, lactic acid bacterium abundance, and digestive enzyme activities of B. gonionotus.
34793569	5	15	from	growth	954:959	arg1	fish					987:990	fish	987:990	fish	987:990	Moreover, dietary chitosan significantly inhibited the growth of pathogenic bacteria in fish.
34793569	0	16	theme	internal	108:115	arg1	morphology					123:132	internal organ morphology	108:132	internal organ morphology	108:132	Dietary chitosan promotes the growth, biochemical composition, gut microbiota, hematological parameters and internal organ morphology of juvenile Barbonymus gonionotus.
34793569	8	17	from	use	1685:1687	arg1	aquaculture					1692:1702	aquaculture	1692:1702	aquaculture	1692:1702	Taken together, our results suggest that the application of dietary chitosan at a dose of 1 g kg-1 feed produced the highest benefit to treated B. gonionotus, indicating its potential for safe use in aquaculture.
34793569	4	18	theme	hematological	790:802	arg1	parameters					804:813	hematological parameters	790:813	hematological parameters	790:813	Comparing the effects of the dietary chitosan-containing diets with those of the control diet, we found that dietary chitosan significantly improved the muscle growth, nutrient and mineral contents, hematological parameters, lactic acid bacterium abundance, and digestive enzyme activities of B. gonionotus.
34793569	7	19	theme	villi	1303:1307	arg1	length					1282:1287	the length	1278:1287	the length of intestinal villi	1278:1307	Quantitative study revealed that dietary chitosan significantly enhanced the length of intestinal villi, and qualitative study showed that dietary chitosan considerably reduced the fat content in the liver and improved the morphology of the kidney compared to those with the basal diet.
34793569	6	20	theme	lipid	1151:1155	arg1	contents					1157:1164	the lipid contents	1147:1164	the lipid contents	1147:1164	Interestingly, an increase in the dietary chitosan level significantly enhanced the protein contents of the muscles and inversely significantly decreased the lipid contents compared to those with the basal diet.
34793569	6	21	theme	dietary	1027:1033	arg1	level					1044:1048	the dietary chitosan level	1023:1048	the dietary chitosan level	1023:1048	Interestingly, an increase in the dietary chitosan level significantly enhanced the protein contents of the muscles and inversely significantly decreased the lipid contents compared to those with the basal diet.
34793569	2	22	theme	different	401:409	arg1	feed					446:449	1, 2, and 3 g kg-1 feed	427:449	1, 2, and 3 g kg-1 feed	427:449	Three test diets containing three different concentrations (1, 2, and 3 g kg-1 feed) of dietary chitosan were formulated.
34793569	2	22	theme	different	401:409	arg1	concentrations					411:424	three different concentrations	395:424	three different concentrations (1, 2, and 3 g kg-1 feed) of dietary chitosan	395:470	Three test diets containing three different concentrations (1, 2, and 3 g kg-1 feed) of dietary chitosan were formulated.
34793569	2	23	theme	g	439:439	arg1	feed					446:449	1, 2, and 3 g kg-1 feed	427:449	1, 2, and 3 g kg-1 feed	427:449	Three test diets containing three different concentrations (1, 2, and 3 g kg-1 feed) of dietary chitosan were formulated.
34793569	2	23	theme	g	439:439	arg1	concentrations					411:424	three different concentrations	395:424	three different concentrations (1, 2, and 3 g kg-1 feed) of dietary chitosan	395:470	Three test diets containing three different concentrations (1, 2, and 3 g kg-1 feed) of dietary chitosan were formulated.
34793569	4	24	theme	gonionotus	887:896	arg1	growth					751:756	the muscle growth	740:756	the muscle growth	740:756	Comparing the effects of the dietary chitosan-containing diets with those of the control diet, we found that dietary chitosan significantly improved the muscle growth, nutrient and mineral contents, hematological parameters, lactic acid bacterium abundance, and digestive enzyme activities of B. gonionotus.
34793569	4	24	theme	gonionotus	887:896	arg1	parameters					804:813	hematological parameters	790:813	hematological parameters	790:813	Comparing the effects of the dietary chitosan-containing diets with those of the control diet, we found that dietary chitosan significantly improved the muscle growth, nutrient and mineral contents, hematological parameters, lactic acid bacterium abundance, and digestive enzyme activities of B. gonionotus.
34793569	4	24	theme	gonionotus	887:896	arg1	nutrient					759:766	nutrient	759:766	nutrient	759:766	Comparing the effects of the dietary chitosan-containing diets with those of the control diet, we found that dietary chitosan significantly improved the muscle growth, nutrient and mineral contents, hematological parameters, lactic acid bacterium abundance, and digestive enzyme activities of B. gonionotus.
34793569	4	24	theme	gonionotus	887:896	arg1	contents					780:787	mineral contents	772:787	mineral contents	772:787	Comparing the effects of the dietary chitosan-containing diets with those of the control diet, we found that dietary chitosan significantly improved the muscle growth, nutrient and mineral contents, hematological parameters, lactic acid bacterium abundance, and digestive enzyme activities of B. gonionotus.
34793569	4	24	theme	gonionotus	887:896	arg1	activities					870:879	digestive enzyme activities	853:879	digestive enzyme activities	853:879	Comparing the effects of the dietary chitosan-containing diets with those of the control diet, we found that dietary chitosan significantly improved the muscle growth, nutrient and mineral contents, hematological parameters, lactic acid bacterium abundance, and digestive enzyme activities of B. gonionotus.
34793569	4	24	theme	gonionotus	887:896	arg1	abundance					838:846	lactic acid bacterium abundance	816:846	lactic acid bacterium abundance	816:846	Comparing the effects of the dietary chitosan-containing diets with those of the control diet, we found that dietary chitosan significantly improved the muscle growth, nutrient and mineral contents, hematological parameters, lactic acid bacterium abundance, and digestive enzyme activities of B. gonionotus.
34793569	6	25	theme	chitosan	1035:1042	arg1	level					1044:1048	the dietary chitosan level	1023:1048	the dietary chitosan level	1023:1048	Interestingly, an increase in the dietary chitosan level significantly enhanced the protein contents of the muscles and inversely significantly decreased the lipid contents compared to those with the basal diet.
34793569	7	26	theme	dietary	1238:1244	arg1	chitosan					1246:1253	dietary chitosan	1238:1253	dietary chitosan	1238:1253	Quantitative study revealed that dietary chitosan significantly enhanced the length of intestinal villi, and qualitative study showed that dietary chitosan considerably reduced the fat content in the liver and improved the morphology of the kidney compared to those with the basal diet.
34793569	4	27	theme	dietary	620:626	arg1	diets					648:652	the dietary chitosan-containing diets	616:652	the dietary chitosan-containing diets	616:652	Comparing the effects of the dietary chitosan-containing diets with those of the control diet, we found that dietary chitosan significantly improved the muscle growth, nutrient and mineral contents, hematological parameters, lactic acid bacterium abundance, and digestive enzyme activities of B. gonionotus.
34793569	0	28	theme	Dietary	0:6	arg1	chitosan					8:15	Dietary chitosan	0:15	Dietary chitosan	0:15	Dietary chitosan promotes the growth, biochemical composition, gut microbiota, hematological parameters and internal organ morphology of juvenile Barbonymus gonionotus.
34793569	7	29	from	content	1390:1396	arg1	liver					1405:1409	the liver	1401:1409	the liver	1401:1409	Quantitative study revealed that dietary chitosan significantly enhanced the length of intestinal villi, and qualitative study showed that dietary chitosan considerably reduced the fat content in the liver and improved the morphology of the kidney compared to those with the basal diet.
34793569	1	30	theme	gut	270:272	arg1	microbiota					274:283	gut microbiota	270:283	gut microbiota	270:283	In this study, we determined the effects of dietary chitosan on the growth, biochemical composition, gut microbiota, and hematological and histological parameters of juvenile Barbonymus gonionotus.
34793569	4	31	theme	mineral	772:778	arg1	contents					780:787	mineral contents	772:787	mineral contents	772:787	Comparing the effects of the dietary chitosan-containing diets with those of the control diet, we found that dietary chitosan significantly improved the muscle growth, nutrient and mineral contents, hematological parameters, lactic acid bacterium abundance, and digestive enzyme activities of B. gonionotus.
34793569	8	32	theme	highest	1609:1615	arg1	benefit					1617:1623	the highest benefit	1605:1623	the highest benefit	1605:1623	Taken together, our results suggest that the application of dietary chitosan at a dose of 1 g kg-1 feed produced the highest benefit to treated B. gonionotus, indicating its potential for safe use in aquaculture.
34793569	8	33	theme	safe	1680:1683	arg1	use					1685:1687	safe use	1680:1687	safe use in aquaculture	1680:1702	Taken together, our results suggest that the application of dietary chitosan at a dose of 1 g kg-1 feed produced the highest benefit to treated B. gonionotus, indicating its potential for safe use in aquaculture.
34793569	7	34	theme	intestinal	1292:1301	arg1	villi					1303:1307	intestinal villi	1292:1307	intestinal villi	1292:1307	Quantitative study revealed that dietary chitosan significantly enhanced the length of intestinal villi, and qualitative study showed that dietary chitosan considerably reduced the fat content in the liver and improved the morphology of the kidney compared to those with the basal diet.
34793569	0	35	theme	organ	117:121	arg1	morphology					123:132	internal organ morphology	108:132	internal organ morphology	108:132	Dietary chitosan promotes the growth, biochemical composition, gut microbiota, hematological parameters and internal organ morphology of juvenile Barbonymus gonionotus.
34793569	5	36	theme	dietary	909:915	arg1	chitosan					917:924	dietary chitosan	909:924	dietary chitosan	909:924	Moreover, dietary chitosan significantly inhibited the growth of pathogenic bacteria in fish.
34793569	4	37	theme	diets	648:652	arg1	effects					605:611	the effects	601:611	the effects of the dietary chitosan-containing diets	601:652	Comparing the effects of the dietary chitosan-containing diets with those of the control diet, we found that dietary chitosan significantly improved the muscle growth, nutrient and mineral contents, hematological parameters, lactic acid bacterium abundance, and digestive enzyme activities of B. gonionotus.
34793569	0	38	theme	juvenile	137:144	arg1	gonionotus					157:166	juvenile Barbonymus gonionotus	137:166	juvenile Barbonymus gonionotus	137:166	Dietary chitosan promotes the growth, biochemical composition, gut microbiota, hematological parameters and internal organ morphology of juvenile Barbonymus gonionotus.
34793569	2	39	contain	containing	384:393	arg1	diets					378:382	Three test diets	367:382	Three test diets containing three different concentrations (1, 2, and 3 g kg-1 feed) of dietary chitosan	367:470	Three test diets containing three different concentrations (1, 2, and 3 g kg-1 feed) of dietary chitosan were formulated.
34793569	2	39	contain	containing	384:393	arg2	feed					446:449	1, 2, and 3 g kg-1 feed	427:449	1, 2, and 3 g kg-1 feed	427:449	Three test diets containing three different concentrations (1, 2, and 3 g kg-1 feed) of dietary chitosan were formulated.
34793569	2	39	contain	containing	384:393	arg2	concentrations					411:424	three different concentrations	395:424	three different concentrations (1, 2, and 3 g kg-1 feed) of dietary chitosan	395:470	Three test diets containing three different concentrations (1, 2, and 3 g kg-1 feed) of dietary chitosan were formulated.
34793569	6	40	dep	enhanced	1064:1071	arg1	decreased					1137:1145	decreased	1137:1145	decreased the lipid contents compared to those with the basal diet	1137:1202	Interestingly, an increase in the dietary chitosan level significantly enhanced the protein contents of the muscles and inversely significantly decreased the lipid contents compared to those with the basal diet.
34793569	7	41	theme	qualitative	1314:1324	arg1	study					1326:1330	qualitative study	1314:1330	qualitative study	1314:1330	Quantitative study revealed that dietary chitosan significantly enhanced the length of intestinal villi, and qualitative study showed that dietary chitosan considerably reduced the fat content in the liver and improved the morphology of the kidney compared to those with the basal diet.
34793569	8	42	theme	dietary	1552:1558	arg1	chitosan					1560:1567	dietary chitosan	1552:1567	dietary chitosan	1552:1567	Taken together, our results suggest that the application of dietary chitosan at a dose of 1 g kg-1 feed produced the highest benefit to treated B. gonionotus, indicating its potential for safe use in aquaculture.
34793569	8	43	from	dose	1574:1577	arg1	application					1537:1547	the application	1533:1547	the application of dietary chitosan at a dose of 1 g kg-1 feed	1533:1594	Taken together, our results suggest that the application of dietary chitosan at a dose of 1 g kg-1 feed produced the highest benefit to treated B. gonionotus, indicating its potential for safe use in aquaculture.
34793569	7	44	theme	fat	1386:1388	arg1	content					1390:1396	the fat content	1382:1396	the fat content in the liver	1382:1409	Quantitative study revealed that dietary chitosan significantly enhanced the length of intestinal villi, and qualitative study showed that dietary chitosan considerably reduced the fat content in the liver and improved the morphology of the kidney compared to those with the basal diet.
34793569	1	45	from	effects	202:208	arg1	microbiota					274:283	gut microbiota	270:283	gut microbiota	270:283	In this study, we determined the effects of dietary chitosan on the growth, biochemical composition, gut microbiota, and hematological and histological parameters of juvenile Barbonymus gonionotus.
34793569	1	45	from	effects	202:208	arg1	parameters					321:330	hematological and histological parameters	290:330	hematological and histological parameters	290:330	In this study, we determined the effects of dietary chitosan on the growth, biochemical composition, gut microbiota, and hematological and histological parameters of juvenile Barbonymus gonionotus.
34793569	1	45	from	effects	202:208	arg1	growth					237:242	growth	237:242	growth	237:242	In this study, we determined the effects of dietary chitosan on the growth, biochemical composition, gut microbiota, and hematological and histological parameters of juvenile Barbonymus gonionotus.
34793569	1	45	from	effects	202:208	arg1	composition					257:267	biochemical composition	245:267	biochemical composition	245:267	In this study, we determined the effects of dietary chitosan on the growth, biochemical composition, gut microbiota, and hematological and histological parameters of juvenile Barbonymus gonionotus.
34793569	8	46	theme	chitosan	1560:1567	arg1	application					1537:1547	the application	1533:1547	the application of dietary chitosan at a dose of 1 g kg-1 feed	1533:1594	Taken together, our results suggest that the application of dietary chitosan at a dose of 1 g kg-1 feed produced the highest benefit to treated B. gonionotus, indicating its potential for safe use in aquaculture.
34793569	2	47	theme	kg-1	441:444	arg1	feed					446:449	1, 2, and 3 g kg-1 feed	427:449	1, 2, and 3 g kg-1 feed	427:449	Three test diets containing three different concentrations (1, 2, and 3 g kg-1 feed) of dietary chitosan were formulated.
34793569	2	47	theme	kg-1	441:444	arg1	concentrations					411:424	three different concentrations	395:424	three different concentrations (1, 2, and 3 g kg-1 feed) of dietary chitosan	395:470	Three test diets containing three different concentrations (1, 2, and 3 g kg-1 feed) of dietary chitosan were formulated.
34793569	1	48	theme	hematological	290:302	arg1	parameters					321:330	hematological and histological parameters	290:330	hematological and histological parameters	290:330	In this study, we determined the effects of dietary chitosan on the growth, biochemical composition, gut microbiota, and hematological and histological parameters of juvenile Barbonymus gonionotus.
34793569	0	49	theme	gonionotus	157:166	arg1	microbiota					67:76	gut microbiota	63:76	gut microbiota	63:76	Dietary chitosan promotes the growth, biochemical composition, gut microbiota, hematological parameters and internal organ morphology of juvenile Barbonymus gonionotus.
34793569	0	49	theme	gonionotus	157:166	arg1	parameters					93:102	hematological parameters	79:102	hematological parameters	79:102	Dietary chitosan promotes the growth, biochemical composition, gut microbiota, hematological parameters and internal organ morphology of juvenile Barbonymus gonionotus.
34793569	0	49	theme	gonionotus	157:166	arg1	morphology					123:132	internal organ morphology	108:132	internal organ morphology	108:132	Dietary chitosan promotes the growth, biochemical composition, gut microbiota, hematological parameters and internal organ morphology of juvenile Barbonymus gonionotus.
34793569	0	49	theme	gonionotus	157:166	arg1	growth					30:35	growth	30:35	growth	30:35	Dietary chitosan promotes the growth, biochemical composition, gut microbiota, hematological parameters and internal organ morphology of juvenile Barbonymus gonionotus.
34793569	0	49	theme	gonionotus	157:166	arg1	composition					50:60	biochemical composition	38:60	biochemical composition	38:60	Dietary chitosan promotes the growth, biochemical composition, gut microbiota, hematological parameters and internal organ morphology of juvenile Barbonymus gonionotus.
34793569	6	50	from	increase	1011:1018	arg1	level					1044:1048	the dietary chitosan level	1023:1048	the dietary chitosan level	1023:1048	Interestingly, an increase in the dietary chitosan level significantly enhanced the protein contents of the muscles and inversely significantly decreased the lipid contents compared to those with the basal diet.
34793569	6	51	theme	protein	1077:1083	arg1	contents					1085:1092	the protein contents	1073:1092	the protein contents of the muscles	1073:1107	Interestingly, an increase in the dietary chitosan level significantly enhanced the protein contents of the muscles and inversely significantly decreased the lipid contents compared to those with the basal diet.
34793569	6	52	theme	muscles	1101:1107	arg1	contents					1085:1092	the protein contents	1073:1092	the protein contents of the muscles	1073:1107	Interestingly, an increase in the dietary chitosan level significantly enhanced the protein contents of the muscles and inversely significantly decreased the lipid contents compared to those with the basal diet.
34793569	0	53	theme	biochemical	38:48	arg1	composition					50:60	biochemical composition	38:60	biochemical composition	38:60	Dietary chitosan promotes the growth, biochemical composition, gut microbiota, hematological parameters and internal organ morphology of juvenile Barbonymus gonionotus.
34793569	0	54	theme	Barbonymus	146:155	arg1	gonionotus					157:166	juvenile Barbonymus gonionotus	137:166	juvenile Barbonymus gonionotus	137:166	Dietary chitosan promotes the growth, biochemical composition, gut microbiota, hematological parameters and internal organ morphology of juvenile Barbonymus gonionotus.
34793569	0	55	dep	growth	30:35	arg1	the					26:28	the	26:28	the	26:28	Dietary chitosan promotes the growth, biochemical composition, gut microbiota, hematological parameters and internal organ morphology of juvenile Barbonymus gonionotus.
34793569	6	56	theme	basal	1193:1197	arg1	diet					1199:1202	the basal diet	1189:1202	the basal diet	1189:1202	Interestingly, an increase in the dietary chitosan level significantly enhanced the protein contents of the muscles and inversely significantly decreased the lipid contents compared to those with the basal diet.
34793569	1	57	theme	histological	308:319	arg1	parameters					321:330	hematological and histological parameters	290:330	hematological and histological parameters	290:330	In this study, we determined the effects of dietary chitosan on the growth, biochemical composition, gut microbiota, and hematological and histological parameters of juvenile Barbonymus gonionotus.
34793569	4	58	theme	enzyme	863:868	arg1	activities					870:879	digestive enzyme activities	853:879	digestive enzyme activities	853:879	Comparing the effects of the dietary chitosan-containing diets with those of the control diet, we found that dietary chitosan significantly improved the muscle growth, nutrient and mineral contents, hematological parameters, lactic acid bacterium abundance, and digestive enzyme activities of B. gonionotus.
34793569	7	59	theme	basal	1480:1484	arg1	diet					1486:1489	the basal diet	1476:1489	the basal diet	1476:1489	Quantitative study revealed that dietary chitosan significantly enhanced the length of intestinal villi, and qualitative study showed that dietary chitosan considerably reduced the fat content in the liver and improved the morphology of the kidney compared to those with the basal diet.
34793569	4	60	theme	muscle	744:749	arg1	growth					751:756	the muscle growth	740:756	the muscle growth	740:756	Comparing the effects of the dietary chitosan-containing diets with those of the control diet, we found that dietary chitosan significantly improved the muscle growth, nutrient and mineral contents, hematological parameters, lactic acid bacterium abundance, and digestive enzyme activities of B. gonionotus.
34793569	4	61	theme	chitosan-containing	628:646	arg1	diets					648:652	the dietary chitosan-containing diets	616:652	the dietary chitosan-containing diets	616:652	Comparing the effects of the dietary chitosan-containing diets with those of the control diet, we found that dietary chitosan significantly improved the muscle growth, nutrient and mineral contents, hematological parameters, lactic acid bacterium abundance, and digestive enzyme activities of B. gonionotus.
34793569	1	62	theme	dietary	213:219	arg1	chitosan					221:228	dietary chitosan	213:228	dietary chitosan	213:228	In this study, we determined the effects of dietary chitosan on the growth, biochemical composition, gut microbiota, and hematological and histological parameters of juvenile Barbonymus gonionotus.
34793569	4	63	theme	digestive	853:861	arg1	activities					870:879	digestive enzyme activities	853:879	digestive enzyme activities	853:879	Comparing the effects of the dietary chitosan-containing diets with those of the control diet, we found that dietary chitosan significantly improved the muscle growth, nutrient and mineral contents, hematological parameters, lactic acid bacterium abundance, and digestive enzyme activities of B. gonionotus.
34793569	1	64	dep	growth	237:242	arg1	the					233:235	the	233:235	the	233:235	In this study, we determined the effects of dietary chitosan on the growth, biochemical composition, gut microbiota, and hematological and histological parameters of juvenile Barbonymus gonionotus.
34793569	7	65	theme	dietary	1344:1350	arg1	chitosan					1352:1359	dietary chitosan	1344:1359	dietary chitosan	1344:1359	Quantitative study revealed that dietary chitosan significantly enhanced the length of intestinal villi, and qualitative study showed that dietary chitosan considerably reduced the fat content in the liver and improved the morphology of the kidney compared to those with the basal diet.
34793569	4	66	theme	control	672:678	arg1	diet					680:683	the control diet	668:683	the control diet	668:683	Comparing the effects of the dietary chitosan-containing diets with those of the control diet, we found that dietary chitosan significantly improved the muscle growth, nutrient and mineral contents, hematological parameters, lactic acid bacterium abundance, and digestive enzyme activities of B. gonionotus.
34793569	1	67	theme	chitosan	221:228	arg1	effects					202:208	the effects	198:208	the effects of dietary chitosan on the growth, biochemical composition, gut microbiota, and hematological and histological parameters of juvenile Barbonymus gonionotus	198:364	In this study, we determined the effects of dietary chitosan on the growth, biochemical composition, gut microbiota, and hematological and histological parameters of juvenile Barbonymus gonionotus.
34793569	0	68	theme	gut	63:65	arg1	microbiota					67:76	gut microbiota	63:76	gut microbiota	63:76	Dietary chitosan promotes the growth, biochemical composition, gut microbiota, hematological parameters and internal organ morphology of juvenile Barbonymus gonionotus.
34793569	5	69	theme	pathogenic	964:973	arg1	bacteria					975:982	pathogenic bacteria	964:982	pathogenic bacteria	964:982	Moreover, dietary chitosan significantly inhibited the growth of pathogenic bacteria in fish.
34793569	2	70	theme	test	373:376	arg1	diets					378:382	Three test diets	367:382	Three test diets containing three different concentrations (1, 2, and 3 g kg-1 feed) of dietary chitosan	367:470	Three test diets containing three different concentrations (1, 2, and 3 g kg-1 feed) of dietary chitosan were formulated.
34793569	7	71	theme	Quantitative	1205:1216	arg1	study					1218:1222	Quantitative study	1205:1222	Quantitative study	1205:1222	Quantitative study revealed that dietary chitosan significantly enhanced the length of intestinal villi, and qualitative study showed that dietary chitosan considerably reduced the fat content in the liver and improved the morphology of the kidney compared to those with the basal diet.
34793569	2	72	theme	chitosan	463:470	arg1	feed					446:449	1, 2, and 3 g kg-1 feed	427:449	1, 2, and 3 g kg-1 feed	427:449	Three test diets containing three different concentrations (1, 2, and 3 g kg-1 feed) of dietary chitosan were formulated.
34793569	2	72	theme	chitosan	463:470	arg1	concentrations					411:424	three different concentrations	395:424	three different concentrations (1, 2, and 3 g kg-1 feed) of dietary chitosan	395:470	Three test diets containing three different concentrations (1, 2, and 3 g kg-1 feed) of dietary chitosan were formulated.
34793569	5	73	theme	bacteria	975:982	arg1	growth					954:959	the growth	950:959	the growth of pathogenic bacteria in fish	950:990	Moreover, dietary chitosan significantly inhibited the growth of pathogenic bacteria in fish.
34793569	3	74	theme	basal	491:495	arg1	diet					497:500	A basal diet	489:500	A basal diet without dietary chitosan	489:525	A basal diet without dietary chitosan was considered a control, and the fish were reared for 60 days.
33982524	0	0	theme	different	87:95	arg1	areas					107:111	different producing areas	87:111	different producing areas	87:111	[Analysis of water-soluble nutrients in Lycium barbarum leaves and differences between different producing areas].
33982524	5	1	theme	other	1034:1038	arg1	areas					1040:1044	other areas	1034:1044	other areas	1034:1044	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	6	2	theme	nutrients	1182:1190	arg1	compositions					1139:1150	compositions	1139:1150	compositions	1139:1150	In this study, the compositions and contents of water-soluble nutrients in LBLs were preliminarily clarified, which provided basis for further development and utilization of LBLs resoures.
33982524	6	2	theme	nutrients	1182:1190	arg1	contents					1156:1163	contents	1156:1163	contents	1156:1163	In this study, the compositions and contents of water-soluble nutrients in LBLs were preliminarily clarified, which provided basis for further development and utilization of LBLs resoures.
33982524	1	3	from	differences	168:178	arg1	areas					254:258	different areas	244:258	different areas	244:258	This study is to clarify the composition and content differences of water-soluble nutrients in Lycium barbarum leaves(LBLs) from different areas.
33982524	1	3	from	differences	168:178	arg1	barbarum					217:224	Lycium barbarum	210:224	Lycium barbarum leaves(LBLs) from different areas	210:258	This study is to clarify the composition and content differences of water-soluble nutrients in Lycium barbarum leaves(LBLs) from different areas.
33982524	1	4	theme	composition	144:154	arg1	differences					168:178	the composition and content differences	140:178	the composition and content differences of water-soluble nutrients in Lycium barbarum leaves(LBLs) from different areas	140:258	This study is to clarify the composition and content differences of water-soluble nutrients in Lycium barbarum leaves(LBLs) from different areas.
33982524	5	5	from	guanosine	1109:1117	arg1	nutrients					942:950	the water-soluble nutrients	924:950	the water-soluble nutrients of the samples from Qinghai Province	924:987	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	5	from	guanosine	1109:1117	arg1	different					1008:1016	different	1008:1016	different	1008:1016	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	6	from	proline	1068:1074	arg1	nutrients					942:950	the water-soluble nutrients	924:950	the water-soluble nutrients of the samples from Qinghai Province	924:987	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	6	from	proline	1068:1074	arg1	different					1008:1016	different	1008:1016	different	1008:1016	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	2	7	from	nucleosides	331:341	arg1	batches					365:371	35 batches	362:371	35 batches of LBLs	362:379	The total polysaccharides, free monosaccharides and oligosaccharides, nucleosides and amino acids in 35 batches of LBLs were analyzed with use of spectrophotometry, HPLC-ELSD and UPLC-MS/MS.
33982524	2	8	from	oligosaccharides	313:328	arg1	batches					365:371	35 batches	362:371	35 batches of LBLs	362:379	The total polysaccharides, free monosaccharides and oligosaccharides, nucleosides and amino acids in 35 batches of LBLs were analyzed with use of spectrophotometry, HPLC-ELSD and UPLC-MS/MS.
33982524	1	9	theme	different	244:252	arg1	areas					254:258	different areas	244:258	different areas	244:258	This study is to clarify the composition and content differences of water-soluble nutrients in Lycium barbarum leaves(LBLs) from different areas.
33982524	5	10	from	Province	980:987	arg1	nutrients					942:950	the water-soluble nutrients	924:950	the water-soluble nutrients of the samples from Qinghai Province	924:987	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	10	from	Province	980:987	arg1	samples					959:965	the samples	955:965	the samples from Qinghai Province	955:987	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	10	from	Province	980:987	arg1	different					1008:1016	different	1008:1016	different	1008:1016	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	0	11	from	[Analysis	0:8	arg1	barbarum					47:54	Lycium barbarum	40:54	Lycium barbarum	40:54	[Analysis of water-soluble nutrients in Lycium barbarum leaves and differences between different producing areas].
33982524	5	12	from	different	1008:1016	arg1	guanosine					1109:1117	guanosine	1109:1117	guanosine	1109:1117	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	12	from	different	1008:1016	arg1	glutamine					1077:1085	glutamine	1077:1085	glutamine	1077:1085	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	12	from	different	1008:1016	arg1	sucrose					1088:1094	sucrose	1088:1094	sucrose	1088:1094	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	12	from	different	1008:1016	arg1	asparagine					1056:1065	asparagine	1056:1065	asparagine	1056:1065	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	12	from	different	1008:1016	arg1	proline					1068:1074	proline	1068:1074	proline	1068:1074	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	12	from	different	1008:1016	arg1	adenine					1097:1103	adenine	1097:1103	adenine	1097:1103	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	0	13	theme	producing	97:105	arg1	areas					107:111	different producing areas	87:111	different producing areas	87:111	[Analysis of water-soluble nutrients in Lycium barbarum leaves and differences between different producing areas].
33982524	5	14	from	asparagine	1056:1065	arg1	nutrients					942:950	the water-soluble nutrients	924:950	the water-soluble nutrients of the samples from Qinghai Province	924:987	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	14	from	asparagine	1056:1065	arg1	different					1008:1016	different	1008:1016	different	1008:1016	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	15	from	nutrients	942:950	arg1	Province					980:987	Province	980:987	Province	980:987	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	6	16	from	compositions	1139:1150	arg1	LBLs					1195:1198	LBLs	1195:1198	LBLs	1195:1198	In this study, the compositions and contents of water-soluble nutrients in LBLs were preliminarily clarified, which provided basis for further development and utilization of LBLs resoures.
33982524	2	17	theme	total	265:269	arg1	polysaccharides					271:285	The total polysaccharides	261:285	The total polysaccharides	261:285	The total polysaccharides, free monosaccharides and oligosaccharides, nucleosides and amino acids in 35 batches of LBLs were analyzed with use of spectrophotometry, HPLC-ELSD and UPLC-MS/MS.
33982524	5	18	theme	Principal	777:785	arg1	PCA					806:808	PCA	806:808	PCA	806:808	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	18	theme	Principal	777:785	arg1	analysis					797:804	Principal component analysis	777:804	Principal component analysis(PCA)	777:809	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	1	19	theme	content	160:166	arg1	differences					168:178	the composition and content differences	140:178	the composition and content differences of water-soluble nutrients in Lycium barbarum leaves(LBLs) from different areas	140:258	This study is to clarify the composition and content differences of water-soluble nutrients in Lycium barbarum leaves(LBLs) from different areas.
33982524	6	20	dep	compositions	1139:1150	arg1	the					1135:1137	the	1135:1137	the	1135:1137	In this study, the compositions and contents of water-soluble nutrients in LBLs were preliminarily clarified, which provided basis for further development and utilization of LBLs resoures.
33982524	5	21	from	sucrose	1088:1094	arg1	nutrients					942:950	the water-soluble nutrients	924:950	the water-soluble nutrients of the samples from Qinghai Province	924:987	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	21	from	sucrose	1088:1094	arg1	different					1008:1016	different	1008:1016	different	1008:1016	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	22	theme	discrimination	837:850	arg1	PLS-DA					861:866	PLS-DA	861:866	PLS-DA	861:866	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	22	theme	discrimination	837:850	arg1	analysis					852:859	partial least squares discrimination analysis	815:859	partial least squares discrimination analysis(PLS-DA)	815:867	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	2	23	theme	UPLC-MS/MS	440:449	arg1	use					400:402	use	400:402	use of spectrophotometry, HPLC-ELSD and UPLC-MS/MS	400:449	The total polysaccharides, free monosaccharides and oligosaccharides, nucleosides and amino acids in 35 batches of LBLs were analyzed with use of spectrophotometry, HPLC-ELSD and UPLC-MS/MS.
33982524	3	24	contain	contained	481:489	arg2	glucose					527:533	glucose	527:533	glucose	527:533	The results showed that LBLs contained abundant polysaccharides, fructose, glucose, sucrose and maltose, with an average contents of 39.07, 12.69, 8.99, 17.44, 8.32 mg·g~(-1), respectively.
33982524	3	24	contain	contained	481:489	arg2	polysaccharides					500:514	abundant polysaccharides	491:514	abundant polysaccharides	491:514	The results showed that LBLs contained abundant polysaccharides, fructose, glucose, sucrose and maltose, with an average contents of 39.07, 12.69, 8.99, 17.44, 8.32 mg·g~(-1), respectively.
33982524	3	24	contain	contained	481:489	arg1	LBLs					476:479	LBLs	476:479	LBLs	476:479	The results showed that LBLs contained abundant polysaccharides, fructose, glucose, sucrose and maltose, with an average contents of 39.07, 12.69, 8.99, 17.44, 8.32 mg·g~(-1), respectively.
33982524	3	24	contain	contained	481:489	arg2	sucrose					536:542	sucrose	536:542	sucrose	536:542	The results showed that LBLs contained abundant polysaccharides, fructose, glucose, sucrose and maltose, with an average contents of 39.07, 12.69, 8.99, 17.44, 8.32 mg·g~(-1), respectively.
33982524	3	24	contain	contained	481:489	arg2	fructose					517:524	fructose	517:524	fructose	517:524	The results showed that LBLs contained abundant polysaccharides, fructose, glucose, sucrose and maltose, with an average contents of 39.07, 12.69, 8.99, 17.44, 8.32 mg·g~(-1), respectively.
33982524	3	24	contain	contained	481:489	arg2	maltose					548:554	maltose	548:554	maltose	548:554	The results showed that LBLs contained abundant polysaccharides, fructose, glucose, sucrose and maltose, with an average contents of 39.07, 12.69, 8.99, 17.44, 8.32 mg·g~(-1), respectively.
33982524	2	25	from	acids	353:357	arg1	batches					365:371	35 batches	362:371	35 batches of LBLs	362:379	The total polysaccharides, free monosaccharides and oligosaccharides, nucleosides and amino acids in 35 batches of LBLs were analyzed with use of spectrophotometry, HPLC-ELSD and UPLC-MS/MS.
33982524	4	26	theme	amino	680:684	arg1	acids					686:690	twelve amino acids	673:690	twelve amino acids	673:690	Besides, eight nucleosides and twelve amino acids were detected in LBLs, and their average total contents were 54.95, 336.9 μg·g~(-1).
33982524	2	27	theme	LBLs	376:379	arg1	batches					365:371	35 batches	362:371	35 batches of LBLs	362:379	The total polysaccharides, free monosaccharides and oligosaccharides, nucleosides and amino acids in 35 batches of LBLs were analyzed with use of spectrophotometry, HPLC-ELSD and UPLC-MS/MS.
33982524	5	28	from	those	1023:1027	arg1	nutrients					942:950	the water-soluble nutrients	924:950	the water-soluble nutrients of the samples from Qinghai Province	924:987	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	28	from	those	1023:1027	arg1	different					1008:1016	different	1008:1016	different	1008:1016	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	4	29	located	detected	697:704	arg2	nucleosides					657:667	eight nucleosides	651:667	eight nucleosides	651:667	Besides, eight nucleosides and twelve amino acids were detected in LBLs, and their average total contents were 54.95, 336.9 μg·g~(-1).
33982524	4	29	located	detected	697:704	arg1	LBLs					709:712	LBLs	709:712	LBLs	709:712	Besides, eight nucleosides and twelve amino acids were detected in LBLs, and their average total contents were 54.95, 336.9 μg·g~(-1).
33982524	4	29	located	detected	697:704	arg2	acids					686:690	twelve amino acids	673:690	twelve amino acids	673:690	Besides, eight nucleosides and twelve amino acids were detected in LBLs, and their average total contents were 54.95, 336.9 μg·g~(-1).
33982524	5	30	from	glutamine	1077:1085	arg1	nutrients					942:950	the water-soluble nutrients	924:950	the water-soluble nutrients of the samples from Qinghai Province	924:987	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	30	from	glutamine	1077:1085	arg1	different					1008:1016	different	1008:1016	different	1008:1016	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	1	31	theme	water-soluble	183:195	arg1	nutrients					197:205	water-soluble nutrients	183:205	water-soluble nutrients	183:205	This study is to clarify the composition and content differences of water-soluble nutrients in Lycium barbarum leaves(LBLs) from different areas.
33982524	2	32	theme	HPLC-ELSD	426:434	arg1	use					400:402	use	400:402	use of spectrophotometry, HPLC-ELSD and UPLC-MS/MS	400:449	The total polysaccharides, free monosaccharides and oligosaccharides, nucleosides and amino acids in 35 batches of LBLs were analyzed with use of spectrophotometry, HPLC-ELSD and UPLC-MS/MS.
33982524	5	33	theme	amino	901:905	arg1	acid					907:910	amino acid	901:910	amino acid	901:910	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	1	34	from	areas	254:258	arg1	differences					168:178	the composition and content differences	140:178	the composition and content differences of water-soluble nutrients in Lycium barbarum leaves(LBLs) from different areas	140:258	This study is to clarify the composition and content differences of water-soluble nutrients in Lycium barbarum leaves(LBLs) from different areas.
33982524	2	35	theme	spectrophotometry	407:423	arg1	use					400:402	use	400:402	use of spectrophotometry, HPLC-ELSD and UPLC-MS/MS	400:449	The total polysaccharides, free monosaccharides and oligosaccharides, nucleosides and amino acids in 35 batches of LBLs were analyzed with use of spectrophotometry, HPLC-ELSD and UPLC-MS/MS.
33982524	0	36	theme	water-soluble	13:25	arg1	nutrients					27:35	water-soluble nutrients	13:35	water-soluble nutrients	13:35	[Analysis of water-soluble nutrients in Lycium barbarum leaves and differences between different producing areas].
33982524	5	37	theme	carbohydrate	872:883	arg1	PCA					806:808	PCA	806:808	PCA	806:808	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	37	theme	carbohydrate	872:883	arg1	analysis					797:804	Principal component analysis	777:804	Principal component analysis(PCA)	777:809	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	37	theme	carbohydrate	872:883	arg1	PLS-DA					861:866	PLS-DA	861:866	PLS-DA	861:866	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	37	theme	carbohydrate	872:883	arg1	analysis					852:859	partial least squares discrimination analysis	815:859	partial least squares discrimination analysis(PLS-DA)	815:867	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	2	38	theme	free	288:291	arg1	monosaccharides					293:307	free monosaccharides	288:307	free monosaccharides	288:307	The total polysaccharides, free monosaccharides and oligosaccharides, nucleosides and amino acids in 35 batches of LBLs were analyzed with use of spectrophotometry, HPLC-ELSD and UPLC-MS/MS.
33982524	5	39	theme	partial	815:821	arg1	PLS-DA					861:866	PLS-DA	861:866	PLS-DA	861:866	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	39	theme	partial	815:821	arg1	analysis					852:859	partial least squares discrimination analysis	815:859	partial least squares discrimination analysis(PLS-DA)	815:867	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	40	theme	component	787:795	arg1	PCA					806:808	PCA	806:808	PCA	806:808	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	40	theme	component	787:795	arg1	analysis					797:804	Principal component analysis	777:804	Principal component analysis(PCA)	777:809	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	41	from	adenine	1097:1103	arg1	nutrients					942:950	the water-soluble nutrients	924:950	the water-soluble nutrients of the samples from Qinghai Province	924:987	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	41	from	adenine	1097:1103	arg1	different					1008:1016	different	1008:1016	different	1008:1016	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	0	42	theme	nutrients	27:35	arg1	[Analysis					0:8	[Analysis	0:8	[Analysis of water-soluble nutrients in Lycium barbarum	0:54	[Analysis of water-soluble nutrients in Lycium barbarum leaves and differences between different producing areas].
33982524	3	43	theme	average	565:571	arg1	contents					573:580	an average contents	562:580	an average contents of 39.07, 12.69, 8.99, 17.44, 8.32 mg·g~(-1)	562:625	The results showed that LBLs contained abundant polysaccharides, fructose, glucose, sucrose and maltose, with an average contents of 39.07, 12.69, 8.99, 17.44, 8.32 mg·g~(-1), respectively.
33982524	2	44	theme	amino	347:351	arg1	acids					353:357	amino acids	347:357	amino acids	347:357	The total polysaccharides, free monosaccharides and oligosaccharides, nucleosides and amino acids in 35 batches of LBLs were analyzed with use of spectrophotometry, HPLC-ELSD and UPLC-MS/MS.
33982524	6	45	theme	resoures	1299:1306	arg1	utilization					1279:1289	utilization	1279:1289	utilization	1279:1289	In this study, the compositions and contents of water-soluble nutrients in LBLs were preliminarily clarified, which provided basis for further development and utilization of LBLs resoures.
33982524	6	45	theme	resoures	1299:1306	arg1	development					1263:1273	further development	1255:1273	further development	1255:1273	In this study, the compositions and contents of water-soluble nutrients in LBLs were preliminarily clarified, which provided basis for further development and utilization of LBLs resoures.
33982524	5	46	theme	water-soluble	928:940	arg1	nutrients					942:950	the water-soluble nutrients	924:950	the water-soluble nutrients of the samples from Qinghai Province	924:987	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	46	theme	water-soluble	928:940	arg1	different					1008:1016	different	1008:1016	different	1008:1016	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	3	47	theme	abundant	491:498	arg1	sucrose					536:542	sucrose	536:542	sucrose	536:542	The results showed that LBLs contained abundant polysaccharides, fructose, glucose, sucrose and maltose, with an average contents of 39.07, 12.69, 8.99, 17.44, 8.32 mg·g~(-1), respectively.
33982524	3	47	theme	abundant	491:498	arg1	glucose					527:533	glucose	527:533	glucose	527:533	The results showed that LBLs contained abundant polysaccharides, fructose, glucose, sucrose and maltose, with an average contents of 39.07, 12.69, 8.99, 17.44, 8.32 mg·g~(-1), respectively.
33982524	3	47	theme	abundant	491:498	arg1	fructose					517:524	fructose	517:524	fructose	517:524	The results showed that LBLs contained abundant polysaccharides, fructose, glucose, sucrose and maltose, with an average contents of 39.07, 12.69, 8.99, 17.44, 8.32 mg·g~(-1), respectively.
33982524	3	47	theme	abundant	491:498	arg1	maltose					548:554	maltose	548:554	maltose	548:554	The results showed that LBLs contained abundant polysaccharides, fructose, glucose, sucrose and maltose, with an average contents of 39.07, 12.69, 8.99, 17.44, 8.32 mg·g~(-1), respectively.
33982524	3	47	theme	abundant	491:498	arg1	polysaccharides					500:514	abundant polysaccharides	491:514	abundant polysaccharides	491:514	The results showed that LBLs contained abundant polysaccharides, fructose, glucose, sucrose and maltose, with an average contents of 39.07, 12.69, 8.99, 17.44, 8.32 mg·g~(-1), respectively.
33982524	5	48	theme	acid	907:910	arg1	PCA					806:808	PCA	806:808	PCA	806:808	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	48	theme	acid	907:910	arg1	analysis					797:804	Principal component analysis	777:804	Principal component analysis(PCA)	777:809	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	48	theme	acid	907:910	arg1	PLS-DA					861:866	PLS-DA	861:866	PLS-DA	861:866	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	48	theme	acid	907:910	arg1	analysis					852:859	partial least squares discrimination analysis	815:859	partial least squares discrimination analysis(PLS-DA)	815:867	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	6	49	theme	water-soluble	1168:1180	arg1	nutrients					1182:1190	water-soluble nutrients	1168:1190	water-soluble nutrients	1168:1190	In this study, the compositions and contents of water-soluble nutrients in LBLs were preliminarily clarified, which provided basis for further development and utilization of LBLs resoures.
33982524	5	50	theme	nucleoside	886:895	arg1	PCA					806:808	PCA	806:808	PCA	806:808	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	50	theme	nucleoside	886:895	arg1	analysis					797:804	Principal component analysis	777:804	Principal component analysis(PCA)	777:809	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	50	theme	nucleoside	886:895	arg1	PLS-DA					861:866	PLS-DA	861:866	PLS-DA	861:866	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	50	theme	nucleoside	886:895	arg1	analysis					852:859	partial least squares discrimination analysis	815:859	partial least squares discrimination analysis(PLS-DA)	815:867	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	51	theme	least	823:827	arg1	PLS-DA					861:866	PLS-DA	861:866	PLS-DA	861:866	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	51	theme	least	823:827	arg1	analysis					852:859	partial least squares discrimination analysis	815:859	partial least squares discrimination analysis(PLS-DA)	815:867	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	3	52	theme	mg·g~	617:621	arg1	contents					573:580	an average contents	562:580	an average contents of 39.07, 12.69, 8.99, 17.44, 8.32 mg·g~(-1)	562:625	The results showed that LBLs contained abundant polysaccharides, fructose, glucose, sucrose and maltose, with an average contents of 39.07, 12.69, 8.99, 17.44, 8.32 mg·g~(-1), respectively.
33982524	1	53	dep	barbarum	217:224	arg1	LBLs					233:236	LBLs	233:236	LBLs	233:236	This study is to clarify the composition and content differences of water-soluble nutrients in Lycium barbarum leaves(LBLs) from different areas.
33982524	1	53	dep	barbarum	217:224	arg1	leaves					226:231	leaves	226:231	leaves	226:231	This study is to clarify the composition and content differences of water-soluble nutrients in Lycium barbarum leaves(LBLs) from different areas.
33982524	5	54	theme	squares	829:835	arg1	PLS-DA					861:866	PLS-DA	861:866	PLS-DA	861:866	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	54	theme	squares	829:835	arg1	analysis					852:859	partial least squares discrimination analysis	815:859	partial least squares discrimination analysis(PLS-DA)	815:867	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	6	55	from	contents	1156:1163	arg1	LBLs					1195:1198	LBLs	1195:1198	LBLs	1195:1198	In this study, the compositions and contents of water-soluble nutrients in LBLs were preliminarily clarified, which provided basis for further development and utilization of LBLs resoures.
33982524	6	56	theme	LBLs	1294:1297	arg1	resoures					1299:1306	LBLs resoures	1294:1306	LBLs resoures	1294:1306	In this study, the compositions and contents of water-soluble nutrients in LBLs were preliminarily clarified, which provided basis for further development and utilization of LBLs resoures.
33982524	5	57	theme	samples	959:965	arg1	nutrients					942:950	the water-soluble nutrients	924:950	the water-soluble nutrients of the samples from Qinghai Province	924:987	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	5	57	theme	samples	959:965	arg1	different					1008:1016	different	1008:1016	different	1008:1016	Principal component analysis(PCA) and partial least squares discrimination analysis(PLS-DA) of carbohydrate, nucleoside and amino acid showed that the water-soluble nutrients of the samples from Qinghai Province were significantly different from those from other areas mainly in asparagine, proline, glutamine, sucrose, adenine and guanosine.
33982524	4	58	theme	total	733:737	arg1	contents					739:746	their average total contents	719:746	their average total contents	719:746	Besides, eight nucleosides and twelve amino acids were detected in LBLs, and their average total contents were 54.95, 336.9 μg·g~(-1).
33982524	1	59	theme	nutrients	197:205	arg1	differences					168:178	the composition and content differences	140:178	the composition and content differences of water-soluble nutrients in Lycium barbarum leaves(LBLs) from different areas	140:258	This study is to clarify the composition and content differences of water-soluble nutrients in Lycium barbarum leaves(LBLs) from different areas.
33982524	2	60	from	polysaccharides	271:285	arg1	batches					365:371	35 batches	362:371	35 batches of LBLs	362:379	The total polysaccharides, free monosaccharides and oligosaccharides, nucleosides and amino acids in 35 batches of LBLs were analyzed with use of spectrophotometry, HPLC-ELSD and UPLC-MS/MS.
33982524	4	61	theme	average	725:731	arg1	contents					739:746	their average total contents	719:746	their average total contents	719:746	Besides, eight nucleosides and twelve amino acids were detected in LBLs, and their average total contents were 54.95, 336.9 μg·g~(-1).
33982524	2	62	from	monosaccharides	293:307	arg1	batches					365:371	35 batches	362:371	35 batches of LBLs	362:379	The total polysaccharides, free monosaccharides and oligosaccharides, nucleosides and amino acids in 35 batches of LBLs were analyzed with use of spectrophotometry, HPLC-ELSD and UPLC-MS/MS.
33982524	6	63	theme	further	1255:1261	arg1	development					1263:1273	further development	1255:1273	further development	1255:1273	In this study, the compositions and contents of water-soluble nutrients in LBLs were preliminarily clarified, which provided basis for further development and utilization of LBLs resoures.
32816067	4	0	theme	osteogenic	769:778	arg1	cells					696:700	the cells	692:700	the cells (rat bone marrow mesenchymal stem cells, mBMSCs) proliferation and osteogenic differentiation on the BG/Gel/SA scaffolds	692:821	Furthermore, the cells (rat bone marrow mesenchymal stem cells, mBMSCs) proliferation and osteogenic differentiation on the BG/Gel/SA scaffolds were also promoted with the increase of bioglass content.
32816067	4	0	theme	osteogenic	769:778	arg1	differentiation					780:794	osteogenic differentiation	769:794	osteogenic differentiation	769:794	Furthermore, the cells (rat bone marrow mesenchymal stem cells, mBMSCs) proliferation and osteogenic differentiation on the BG/Gel/SA scaffolds were also promoted with the increase of bioglass content.
32816067	5	1	theme	potential	1035:1043	arg1	applications					1045:1056	potential applications	1035:1056	potential applications in bone tissue engineering and bone regenerative repair	1035:1112	Overall, the adding of bioglass in Gel/SA scaffolds promotes mechanical strength and in vitro osteogenic properties and the 30 BG scaffold (30%wt BG) has potential applications in bone tissue engineering and bone regenerative repair because of good compressive strength, biocompatibility, and in vitro osteogenesis.
32816067	1	2	theme	porous	174:179	arg1	scaffolds					231:239	porous bioglass/gelatin/alginate bone tissue engineering scaffolds	174:239	porous bioglass/gelatin/alginate bone tissue engineering scaffolds	174:239	In this study, porous bioglass/gelatin/alginate bone tissue engineering scaffolds were fabricated by three-dimensional printing.
32816067	5	3	theme	osteogenic	975:984	arg1	properties					986:995	in vitro osteogenic properties	966:995	in vitro osteogenic properties	966:995	Overall, the adding of bioglass in Gel/SA scaffolds promotes mechanical strength and in vitro osteogenic properties and the 30 BG scaffold (30%wt BG) has potential applications in bone tissue engineering and bone regenerative repair because of good compressive strength, biocompatibility, and in vitro osteogenesis.
32816067	1	4	theme	bioglass/gelatin/alginate	181:205	arg1	scaffolds					231:239	porous bioglass/gelatin/alginate bone tissue engineering scaffolds	174:239	porous bioglass/gelatin/alginate bone tissue engineering scaffolds	174:239	In this study, porous bioglass/gelatin/alginate bone tissue engineering scaffolds were fabricated by three-dimensional printing.
32816067	0	5	theme	cell	126:129	arg1	responses					131:139	cell responses	126:139	cell responses	126:139	Three dimensional printed bioglass/gelatin/alginate composite scaffolds with promoted mechanical strength, biomineralization, cell responses and osteogenesis.
32816067	4	6	theme	bioglass	863:870	arg1	content					872:878	bioglass content	863:878	bioglass content	863:878	Furthermore, the cells (rat bone marrow mesenchymal stem cells, mBMSCs) proliferation and osteogenic differentiation on the BG/Gel/SA scaffolds were also promoted with the increase of bioglass content.
32816067	5	7	theme	tissue	1066:1071	arg1	engineering					1073:1083	bone tissue engineering	1061:1083	bone tissue engineering	1061:1083	Overall, the adding of bioglass in Gel/SA scaffolds promotes mechanical strength and in vitro osteogenic properties and the 30 BG scaffold (30%wt BG) has potential applications in bone tissue engineering and bone regenerative repair because of good compressive strength, biocompatibility, and in vitro osteogenesis.
32816067	3	8	theme	BG/Gel/SA	603:611	arg1	surfaces					623:630	the BG/Gel/SA scaffolds surfaces	599:630	the BG/Gel/SA scaffolds surfaces	599:630	In addition, the cells attach and spread on the BG/Gel/SA scaffolds surfaces represents good adhesion and biocompatibility.
32816067	5	9	theme	%	1023:1023	arg1	BG					1027:1028	30%wt BG	1021:1028	30%wt BG	1021:1028	Overall, the adding of bioglass in Gel/SA scaffolds promotes mechanical strength and in vitro osteogenic properties and the 30 BG scaffold (30%wt BG) has potential applications in bone tissue engineering and bone regenerative repair because of good compressive strength, biocompatibility, and in vitro osteogenesis.
32816067	5	9	theme	%	1023:1023	arg1	scaffold					1011:1018	the 30 BG scaffold	1001:1018	the 30 BG scaffold (30%wt BG)	1001:1029	Overall, the adding of bioglass in Gel/SA scaffolds promotes mechanical strength and in vitro osteogenic properties and the 30 BG scaffold (30%wt BG) has potential applications in bone tissue engineering and bone regenerative repair because of good compressive strength, biocompatibility, and in vitro osteogenesis.
32816067	3	10	theme	good	643:646	arg1	adhesion					648:655	good adhesion	643:655	good adhesion	643:655	In addition, the cells attach and spread on the BG/Gel/SA scaffolds surfaces represents good adhesion and biocompatibility.
32816067	4	11	theme	rat	703:705	arg1	cells					736:740	rat bone marrow mesenchymal stem cells	703:740	rat bone marrow mesenchymal stem cells	703:740	Furthermore, the cells (rat bone marrow mesenchymal stem cells, mBMSCs) proliferation and osteogenic differentiation on the BG/Gel/SA scaffolds were also promoted with the increase of bioglass content.
32816067	4	11	theme	rat	703:705	arg1	mBMSCs					743:748	mBMSCs	743:748	mBMSCs	743:748	Furthermore, the cells (rat bone marrow mesenchymal stem cells, mBMSCs) proliferation and osteogenic differentiation on the BG/Gel/SA scaffolds were also promoted with the increase of bioglass content.
32816067	3	12	theme	scaffolds	613:621	arg1	surfaces					623:630	the BG/Gel/SA scaffolds surfaces	599:630	the BG/Gel/SA scaffolds surfaces	599:630	In addition, the cells attach and spread on the BG/Gel/SA scaffolds surfaces represents good adhesion and biocompatibility.
32816067	5	13	contain	has	1031:1033	arg1	BG					1027:1028	30%wt BG	1021:1028	30%wt BG	1021:1028	Overall, the adding of bioglass in Gel/SA scaffolds promotes mechanical strength and in vitro osteogenic properties and the 30 BG scaffold (30%wt BG) has potential applications in bone tissue engineering and bone regenerative repair because of good compressive strength, biocompatibility, and in vitro osteogenesis.
32816067	5	13	contain	has	1031:1033	arg1	scaffold					1011:1018	the 30 BG scaffold	1001:1018	the 30 BG scaffold (30%wt BG)	1001:1029	Overall, the adding of bioglass in Gel/SA scaffolds promotes mechanical strength and in vitro osteogenic properties and the 30 BG scaffold (30%wt BG) has potential applications in bone tissue engineering and bone regenerative repair because of good compressive strength, biocompatibility, and in vitro osteogenesis.
32816067	5	13	contain	has	1031:1033	arg2	applications					1045:1056	potential applications	1035:1056	potential applications in bone tissue engineering and bone regenerative repair	1035:1112	Overall, the adding of bioglass in Gel/SA scaffolds promotes mechanical strength and in vitro osteogenic properties and the 30 BG scaffold (30%wt BG) has potential applications in bone tissue engineering and bone regenerative repair because of good compressive strength, biocompatibility, and in vitro osteogenesis.
32816067	2	14	from	decline	534:540	arg1	strength					545:552	strength	545:552	strength	545:552	The compressive strength and in vitro biomineralization properties of the bioglass-gelatin-alginate scaffolds (BG/Gel/SA scaffolds) were significantly improved with the increase of bioglass content until 30% weight percentage followed by a rapid decline in strength.
32816067	3	15	from	spread	589:594	arg1	surfaces					623:630	the BG/Gel/SA scaffolds surfaces	599:630	the BG/Gel/SA scaffolds surfaces	599:630	In addition, the cells attach and spread on the BG/Gel/SA scaffolds surfaces represents good adhesion and biocompatibility.
32816067	1	16	theme	engineering	219:229	arg1	scaffolds					231:239	porous bioglass/gelatin/alginate bone tissue engineering scaffolds	174:239	porous bioglass/gelatin/alginate bone tissue engineering scaffolds	174:239	In this study, porous bioglass/gelatin/alginate bone tissue engineering scaffolds were fabricated by three-dimensional printing.
32816067	1	17	theme	bone	207:210	arg1	scaffolds					231:239	porous bioglass/gelatin/alginate bone tissue engineering scaffolds	174:239	porous bioglass/gelatin/alginate bone tissue engineering scaffolds	174:239	In this study, porous bioglass/gelatin/alginate bone tissue engineering scaffolds were fabricated by three-dimensional printing.
32816067	2	18	theme	biomineralization	326:342	arg1	properties					344:353	in vitro biomineralization properties	317:353	in vitro biomineralization properties	317:353	The compressive strength and in vitro biomineralization properties of the bioglass-gelatin-alginate scaffolds (BG/Gel/SA scaffolds) were significantly improved with the increase of bioglass content until 30% weight percentage followed by a rapid decline in strength.
32816067	1	19	theme	tissue	212:217	arg1	scaffolds					231:239	porous bioglass/gelatin/alginate bone tissue engineering scaffolds	174:239	porous bioglass/gelatin/alginate bone tissue engineering scaffolds	174:239	In this study, porous bioglass/gelatin/alginate bone tissue engineering scaffolds were fabricated by three-dimensional printing.
32816067	2	20	theme	rapid	528:532	arg1	decline					534:540	a rapid decline	526:540	a rapid decline in strength	526:552	The compressive strength and in vitro biomineralization properties of the bioglass-gelatin-alginate scaffolds (BG/Gel/SA scaffolds) were significantly improved with the increase of bioglass content until 30% weight percentage followed by a rapid decline in strength.
32816067	4	21	theme	marrow	712:717	arg1	cells					736:740	rat bone marrow mesenchymal stem cells	703:740	rat bone marrow mesenchymal stem cells	703:740	Furthermore, the cells (rat bone marrow mesenchymal stem cells, mBMSCs) proliferation and osteogenic differentiation on the BG/Gel/SA scaffolds were also promoted with the increase of bioglass content.
32816067	4	21	theme	marrow	712:717	arg1	mBMSCs					743:748	mBMSCs	743:748	mBMSCs	743:748	Furthermore, the cells (rat bone marrow mesenchymal stem cells, mBMSCs) proliferation and osteogenic differentiation on the BG/Gel/SA scaffolds were also promoted with the increase of bioglass content.
32816067	4	22	theme	stem	731:734	arg1	cells					736:740	rat bone marrow mesenchymal stem cells	703:740	rat bone marrow mesenchymal stem cells	703:740	Furthermore, the cells (rat bone marrow mesenchymal stem cells, mBMSCs) proliferation and osteogenic differentiation on the BG/Gel/SA scaffolds were also promoted with the increase of bioglass content.
32816067	4	22	theme	stem	731:734	arg1	mBMSCs					743:748	mBMSCs	743:748	mBMSCs	743:748	Furthermore, the cells (rat bone marrow mesenchymal stem cells, mBMSCs) proliferation and osteogenic differentiation on the BG/Gel/SA scaffolds were also promoted with the increase of bioglass content.
32816067	2	23	theme	BG/Gel/SA	399:407	arg1	scaffolds					388:396	the bioglass-gelatin-alginate scaffolds	358:396	the bioglass-gelatin-alginate scaffolds (BG/Gel/SA scaffolds)	358:418	The compressive strength and in vitro biomineralization properties of the bioglass-gelatin-alginate scaffolds (BG/Gel/SA scaffolds) were significantly improved with the increase of bioglass content until 30% weight percentage followed by a rapid decline in strength.
32816067	2	23	theme	BG/Gel/SA	399:407	arg1	scaffolds					409:417	BG/Gel/SA scaffolds	399:417	BG/Gel/SA scaffolds	399:417	The compressive strength and in vitro biomineralization properties of the bioglass-gelatin-alginate scaffolds (BG/Gel/SA scaffolds) were significantly improved with the increase of bioglass content until 30% weight percentage followed by a rapid decline in strength.
32816067	5	24	from	applications	1045:1056	arg1	engineering					1073:1083	bone tissue engineering	1061:1083	bone tissue engineering	1061:1083	Overall, the adding of bioglass in Gel/SA scaffolds promotes mechanical strength and in vitro osteogenic properties and the 30 BG scaffold (30%wt BG) has potential applications in bone tissue engineering and bone regenerative repair because of good compressive strength, biocompatibility, and in vitro osteogenesis.
32816067	5	24	from	applications	1045:1056	arg1	bone					1089:1092	bone	1089:1092	bone	1089:1092	Overall, the adding of bioglass in Gel/SA scaffolds promotes mechanical strength and in vitro osteogenic properties and the 30 BG scaffold (30%wt BG) has potential applications in bone tissue engineering and bone regenerative repair because of good compressive strength, biocompatibility, and in vitro osteogenesis.
32816067	2	25	theme	bioglass	469:476	arg1	content					478:484	bioglass content	469:484	bioglass content	469:484	The compressive strength and in vitro biomineralization properties of the bioglass-gelatin-alginate scaffolds (BG/Gel/SA scaffolds) were significantly improved with the increase of bioglass content until 30% weight percentage followed by a rapid decline in strength.
32816067	5	26	theme	mechanical	942:951	arg1	strength					953:960	mechanical strength	942:960	mechanical strength	942:960	Overall, the adding of bioglass in Gel/SA scaffolds promotes mechanical strength and in vitro osteogenic properties and the 30 BG scaffold (30%wt BG) has potential applications in bone tissue engineering and bone regenerative repair because of good compressive strength, biocompatibility, and in vitro osteogenesis.
32816067	2	27	dep	in	317:318	arg1	vitro					320:324	vitro	320:324	vitro	320:324	The compressive strength and in vitro biomineralization properties of the bioglass-gelatin-alginate scaffolds (BG/Gel/SA scaffolds) were significantly improved with the increase of bioglass content until 30% weight percentage followed by a rapid decline in strength.
32816067	5	28	theme	Gel/SA	916:921	arg1	scaffolds					923:931	Gel/SA scaffolds	916:931	Gel/SA scaffolds	916:931	Overall, the adding of bioglass in Gel/SA scaffolds promotes mechanical strength and in vitro osteogenic properties and the 30 BG scaffold (30%wt BG) has potential applications in bone tissue engineering and bone regenerative repair because of good compressive strength, biocompatibility, and in vitro osteogenesis.
32816067	2	29	theme	scaffolds	388:396	arg1	properties					344:353	in vitro biomineralization properties	317:353	in vitro biomineralization properties	317:353	The compressive strength and in vitro biomineralization properties of the bioglass-gelatin-alginate scaffolds (BG/Gel/SA scaffolds) were significantly improved with the increase of bioglass content until 30% weight percentage followed by a rapid decline in strength.
32816067	2	29	theme	scaffolds	388:396	arg1	strength					304:311	compressive strength	292:311	compressive strength	292:311	The compressive strength and in vitro biomineralization properties of the bioglass-gelatin-alginate scaffolds (BG/Gel/SA scaffolds) were significantly improved with the increase of bioglass content until 30% weight percentage followed by a rapid decline in strength.
32816067	2	30	dep	strength	304:311	arg1	The					288:290	The	288:290	The	288:290	The compressive strength and in vitro biomineralization properties of the bioglass-gelatin-alginate scaffolds (BG/Gel/SA scaffolds) were significantly improved with the increase of bioglass content until 30% weight percentage followed by a rapid decline in strength.
32816067	4	31	theme	bone	707:710	arg1	cells					736:740	rat bone marrow mesenchymal stem cells	703:740	rat bone marrow mesenchymal stem cells	703:740	Furthermore, the cells (rat bone marrow mesenchymal stem cells, mBMSCs) proliferation and osteogenic differentiation on the BG/Gel/SA scaffolds were also promoted with the increase of bioglass content.
32816067	4	31	theme	bone	707:710	arg1	mBMSCs					743:748	mBMSCs	743:748	mBMSCs	743:748	Furthermore, the cells (rat bone marrow mesenchymal stem cells, mBMSCs) proliferation and osteogenic differentiation on the BG/Gel/SA scaffolds were also promoted with the increase of bioglass content.
32816067	5	32	theme	good	1125:1128	arg1	strength					1142:1149	good compressive strength	1125:1149	good compressive strength	1125:1149	Overall, the adding of bioglass in Gel/SA scaffolds promotes mechanical strength and in vitro osteogenic properties and the 30 BG scaffold (30%wt BG) has potential applications in bone tissue engineering and bone regenerative repair because of good compressive strength, biocompatibility, and in vitro osteogenesis.
32816067	2	33	theme	bioglass-gelatin-alginate	362:386	arg1	scaffolds					388:396	the bioglass-gelatin-alginate scaffolds	358:396	the bioglass-gelatin-alginate scaffolds (BG/Gel/SA scaffolds)	358:418	The compressive strength and in vitro biomineralization properties of the bioglass-gelatin-alginate scaffolds (BG/Gel/SA scaffolds) were significantly improved with the increase of bioglass content until 30% weight percentage followed by a rapid decline in strength.
32816067	2	33	theme	bioglass-gelatin-alginate	362:386	arg1	scaffolds					409:417	BG/Gel/SA scaffolds	399:417	BG/Gel/SA scaffolds	399:417	The compressive strength and in vitro biomineralization properties of the bioglass-gelatin-alginate scaffolds (BG/Gel/SA scaffolds) were significantly improved with the increase of bioglass content until 30% weight percentage followed by a rapid decline in strength.
32816067	2	34	theme	weight	496:501	arg1	percentage					503:512	30% weight percentage	492:512	30% weight percentage followed by a rapid decline in strength	492:552	The compressive strength and in vitro biomineralization properties of the bioglass-gelatin-alginate scaffolds (BG/Gel/SA scaffolds) were significantly improved with the increase of bioglass content until 30% weight percentage followed by a rapid decline in strength.
32816067	5	35	theme	wt	1024:1025	arg1	BG					1027:1028	30%wt BG	1021:1028	30%wt BG	1021:1028	Overall, the adding of bioglass in Gel/SA scaffolds promotes mechanical strength and in vitro osteogenic properties and the 30 BG scaffold (30%wt BG) has potential applications in bone tissue engineering and bone regenerative repair because of good compressive strength, biocompatibility, and in vitro osteogenesis.
32816067	5	35	theme	wt	1024:1025	arg1	scaffold					1011:1018	the 30 BG scaffold	1001:1018	the 30 BG scaffold (30%wt BG)	1001:1029	Overall, the adding of bioglass in Gel/SA scaffolds promotes mechanical strength and in vitro osteogenic properties and the 30 BG scaffold (30%wt BG) has potential applications in bone tissue engineering and bone regenerative repair because of good compressive strength, biocompatibility, and in vitro osteogenesis.
32816067	4	36	theme	mesenchymal	719:729	arg1	cells					736:740	rat bone marrow mesenchymal stem cells	703:740	rat bone marrow mesenchymal stem cells	703:740	Furthermore, the cells (rat bone marrow mesenchymal stem cells, mBMSCs) proliferation and osteogenic differentiation on the BG/Gel/SA scaffolds were also promoted with the increase of bioglass content.
32816067	4	36	theme	mesenchymal	719:729	arg1	mBMSCs					743:748	mBMSCs	743:748	mBMSCs	743:748	Furthermore, the cells (rat bone marrow mesenchymal stem cells, mBMSCs) proliferation and osteogenic differentiation on the BG/Gel/SA scaffolds were also promoted with the increase of bioglass content.
32816067	2	37	theme	compressive	292:302	arg1	strength					304:311	compressive strength	292:311	compressive strength	292:311	The compressive strength and in vitro biomineralization properties of the bioglass-gelatin-alginate scaffolds (BG/Gel/SA scaffolds) were significantly improved with the increase of bioglass content until 30% weight percentage followed by a rapid decline in strength.
32816067	5	38	dep	engineering	1073:1083	arg1	repair					1107:1112	regenerative repair	1094:1112	regenerative repair	1094:1112	Overall, the adding of bioglass in Gel/SA scaffolds promotes mechanical strength and in vitro osteogenic properties and the 30 BG scaffold (30%wt BG) has potential applications in bone tissue engineering and bone regenerative repair because of good compressive strength, biocompatibility, and in vitro osteogenesis.
32816067	2	39	theme	%	494:494	arg1	percentage					503:512	30% weight percentage	492:512	30% weight percentage followed by a rapid decline in strength	492:552	The compressive strength and in vitro biomineralization properties of the bioglass-gelatin-alginate scaffolds (BG/Gel/SA scaffolds) were significantly improved with the increase of bioglass content until 30% weight percentage followed by a rapid decline in strength.
32816067	0	40	theme	composite	52:60	arg1	scaffolds					62:70	composite scaffolds	52:70	composite scaffolds with promoted mechanical strength, biomineralization, cell responses and osteogenesis	52:156	Three dimensional printed bioglass/gelatin/alginate composite scaffolds with promoted mechanical strength, biomineralization, cell responses and osteogenesis.
32816067	1	41	theme	three-dimensional	260:276	arg1	printing					278:285	three-dimensional printing	260:285	three-dimensional printing	260:285	In this study, porous bioglass/gelatin/alginate bone tissue engineering scaffolds were fabricated by three-dimensional printing.
32816067	0	42	theme	promoted	77:84	arg1	strength					97:104	promoted mechanical strength	77:104	promoted mechanical strength	77:104	Three dimensional printed bioglass/gelatin/alginate composite scaffolds with promoted mechanical strength, biomineralization, cell responses and osteogenesis.
32816067	5	43	dep	in	1174:1175	arg1	vitro					1177:1181	vitro	1177:1181	vitro	1177:1181	Overall, the adding of bioglass in Gel/SA scaffolds promotes mechanical strength and in vitro osteogenic properties and the 30 BG scaffold (30%wt BG) has potential applications in bone tissue engineering and bone regenerative repair because of good compressive strength, biocompatibility, and in vitro osteogenesis.
32816067	4	44	from	cells	696:700	arg1	scaffolds					813:821	the BG/Gel/SA scaffolds	799:821	the BG/Gel/SA scaffolds	799:821	Furthermore, the cells (rat bone marrow mesenchymal stem cells, mBMSCs) proliferation and osteogenic differentiation on the BG/Gel/SA scaffolds were also promoted with the increase of bioglass content.
32816067	5	45	theme	in	1174:1175	arg1	osteogenesis					1183:1194	in vitro osteogenesis	1174:1194	in vitro osteogenesis	1174:1194	Overall, the adding of bioglass in Gel/SA scaffolds promotes mechanical strength and in vitro osteogenic properties and the 30 BG scaffold (30%wt BG) has potential applications in bone tissue engineering and bone regenerative repair because of good compressive strength, biocompatibility, and in vitro osteogenesis.
32816067	5	46	theme	regenerative	1094:1105	arg1	repair					1107:1112	regenerative repair	1094:1112	regenerative repair	1094:1112	Overall, the adding of bioglass in Gel/SA scaffolds promotes mechanical strength and in vitro osteogenic properties and the 30 BG scaffold (30%wt BG) has potential applications in bone tissue engineering and bone regenerative repair because of good compressive strength, biocompatibility, and in vitro osteogenesis.
32816067	4	47	dep	cells	696:700	arg1	cells					736:740	rat bone marrow mesenchymal stem cells	703:740	rat bone marrow mesenchymal stem cells	703:740	Furthermore, the cells (rat bone marrow mesenchymal stem cells, mBMSCs) proliferation and osteogenic differentiation on the BG/Gel/SA scaffolds were also promoted with the increase of bioglass content.
32816067	4	47	dep	cells	696:700	arg1	cells					696:700	the cells	692:700	the cells (rat bone marrow mesenchymal stem cells, mBMSCs) proliferation and osteogenic differentiation on the BG/Gel/SA scaffolds	692:821	Furthermore, the cells (rat bone marrow mesenchymal stem cells, mBMSCs) proliferation and osteogenic differentiation on the BG/Gel/SA scaffolds were also promoted with the increase of bioglass content.
32816067	4	47	dep	cells	696:700	arg1	proliferation					751:763	proliferation	751:763	proliferation	751:763	Furthermore, the cells (rat bone marrow mesenchymal stem cells, mBMSCs) proliferation and osteogenic differentiation on the BG/Gel/SA scaffolds were also promoted with the increase of bioglass content.
32816067	4	47	dep	cells	696:700	arg1	differentiation					780:794	osteogenic differentiation	769:794	osteogenic differentiation	769:794	Furthermore, the cells (rat bone marrow mesenchymal stem cells, mBMSCs) proliferation and osteogenic differentiation on the BG/Gel/SA scaffolds were also promoted with the increase of bioglass content.
32816067	4	47	dep	cells	696:700	arg1	mBMSCs					743:748	mBMSCs	743:748	mBMSCs	743:748	Furthermore, the cells (rat bone marrow mesenchymal stem cells, mBMSCs) proliferation and osteogenic differentiation on the BG/Gel/SA scaffolds were also promoted with the increase of bioglass content.
32816067	4	48	theme	content	872:878	arg1	increase					851:858	the increase	847:858	the increase of bioglass content	847:878	Furthermore, the cells (rat bone marrow mesenchymal stem cells, mBMSCs) proliferation and osteogenic differentiation on the BG/Gel/SA scaffolds were also promoted with the increase of bioglass content.
32816067	4	49	theme	BG/Gel/SA	803:811	arg1	scaffolds					813:821	the BG/Gel/SA scaffolds	799:821	the BG/Gel/SA scaffolds	799:821	Furthermore, the cells (rat bone marrow mesenchymal stem cells, mBMSCs) proliferation and osteogenic differentiation on the BG/Gel/SA scaffolds were also promoted with the increase of bioglass content.
32816067	5	50	dep	in	966:967	arg1	vitro					969:973	vitro	969:973	vitro	969:973	Overall, the adding of bioglass in Gel/SA scaffolds promotes mechanical strength and in vitro osteogenic properties and the 30 BG scaffold (30%wt BG) has potential applications in bone tissue engineering and bone regenerative repair because of good compressive strength, biocompatibility, and in vitro osteogenesis.
32816067	2	51	theme	in	317:318	arg1	properties					344:353	in vitro biomineralization properties	317:353	in vitro biomineralization properties	317:353	The compressive strength and in vitro biomineralization properties of the bioglass-gelatin-alginate scaffolds (BG/Gel/SA scaffolds) were significantly improved with the increase of bioglass content until 30% weight percentage followed by a rapid decline in strength.
32816067	5	52	theme	compressive	1130:1140	arg1	strength					1142:1149	good compressive strength	1125:1149	good compressive strength	1125:1149	Overall, the adding of bioglass in Gel/SA scaffolds promotes mechanical strength and in vitro osteogenic properties and the 30 BG scaffold (30%wt BG) has potential applications in bone tissue engineering and bone regenerative repair because of good compressive strength, biocompatibility, and in vitro osteogenesis.
32816067	5	53	theme	BG	1008:1009	arg1	BG					1027:1028	30%wt BG	1021:1028	30%wt BG	1021:1028	Overall, the adding of bioglass in Gel/SA scaffolds promotes mechanical strength and in vitro osteogenic properties and the 30 BG scaffold (30%wt BG) has potential applications in bone tissue engineering and bone regenerative repair because of good compressive strength, biocompatibility, and in vitro osteogenesis.
32816067	5	53	theme	BG	1008:1009	arg1	scaffold					1011:1018	the 30 BG scaffold	1001:1018	the 30 BG scaffold (30%wt BG)	1001:1029	Overall, the adding of bioglass in Gel/SA scaffolds promotes mechanical strength and in vitro osteogenic properties and the 30 BG scaffold (30%wt BG) has potential applications in bone tissue engineering and bone regenerative repair because of good compressive strength, biocompatibility, and in vitro osteogenesis.
32816067	5	54	theme	30	1005:1006	arg1	BG					1008:1009	BG	1008:1009	BG	1008:1009	Overall, the adding of bioglass in Gel/SA scaffolds promotes mechanical strength and in vitro osteogenic properties and the 30 BG scaffold (30%wt BG) has potential applications in bone tissue engineering and bone regenerative repair because of good compressive strength, biocompatibility, and in vitro osteogenesis.
32816067	2	55	theme	30	492:493	arg1	%					494:494	%	494:494	%	494:494	The compressive strength and in vitro biomineralization properties of the bioglass-gelatin-alginate scaffolds (BG/Gel/SA scaffolds) were significantly improved with the increase of bioglass content until 30% weight percentage followed by a rapid decline in strength.
32816067	5	56	theme	in	966:967	arg1	properties					986:995	in vitro osteogenic properties	966:995	in vitro osteogenic properties	966:995	Overall, the adding of bioglass in Gel/SA scaffolds promotes mechanical strength and in vitro osteogenic properties and the 30 BG scaffold (30%wt BG) has potential applications in bone tissue engineering and bone regenerative repair because of good compressive strength, biocompatibility, and in vitro osteogenesis.
32816067	2	57	theme	content	478:484	arg1	increase					457:464	the increase	453:464	the increase of bioglass content	453:484	The compressive strength and in vitro biomineralization properties of the bioglass-gelatin-alginate scaffolds (BG/Gel/SA scaffolds) were significantly improved with the increase of bioglass content until 30% weight percentage followed by a rapid decline in strength.
32816067	0	58	theme	mechanical	86:95	arg1	strength					97:104	promoted mechanical strength	77:104	promoted mechanical strength	77:104	Three dimensional printed bioglass/gelatin/alginate composite scaffolds with promoted mechanical strength, biomineralization, cell responses and osteogenesis.
32816067	5	59	theme	bone	1061:1064	arg1	engineering					1073:1083	bone tissue engineering	1061:1083	bone tissue engineering	1061:1083	Overall, the adding of bioglass in Gel/SA scaffolds promotes mechanical strength and in vitro osteogenic properties and the 30 BG scaffold (30%wt BG) has potential applications in bone tissue engineering and bone regenerative repair because of good compressive strength, biocompatibility, and in vitro osteogenesis.
32816067	0	60	with	scaffolds	62:70	arg1	osteogenesis					145:156	osteogenesis	145:156	osteogenesis	145:156	Three dimensional printed bioglass/gelatin/alginate composite scaffolds with promoted mechanical strength, biomineralization, cell responses and osteogenesis.
32816067	0	60	with	scaffolds	62:70	arg1	biomineralization					107:123	biomineralization	107:123	biomineralization	107:123	Three dimensional printed bioglass/gelatin/alginate composite scaffolds with promoted mechanical strength, biomineralization, cell responses and osteogenesis.
32816067	0	60	with	scaffolds	62:70	arg1	responses					131:139	cell responses	126:139	cell responses	126:139	Three dimensional printed bioglass/gelatin/alginate composite scaffolds with promoted mechanical strength, biomineralization, cell responses and osteogenesis.
32816067	0	60	with	scaffolds	62:70	arg1	strength					97:104	promoted mechanical strength	77:104	promoted mechanical strength	77:104	Three dimensional printed bioglass/gelatin/alginate composite scaffolds with promoted mechanical strength, biomineralization, cell responses and osteogenesis.
32553337	8	0	from	life-threatening	1206:1221	arg1	group					1259:1263	the protamine group	1245:1263	the protamine group (0.1% vs. 2.6% [p < 0.001] and 1.0% vs. 4.1% [p = 0.008], respectively)	1245:1335	This was driven mainly by lower rates of life-threatening and major bleeding in the protamine group (0.1% vs. 2.6% [p < 0.001] and 1.0% vs. 4.1% [p = 0.008], respectively).
32553337	4	1	theme	pre-closure	628:638	arg1	devices					640:646	pre-closure devices	628:646	pre-closure devices	628:646	Standard access management included the use of pre-closure devices, manual compression, and percutaneous transluminal angioplasty or implantation of a covered stent graft, if necessary.
32553337	6	2	theme	Written	873:879	arg1	consent					890:896	Written informed consent	873:896	Written informed consent	873:896	Written informed consent was obtained from all patients.
32553337	2	3	theme	major	359:363	arg1	complications					374:386	major bleeding complications	359:386	major bleeding complications	359:386	BACKGROUND Occurrence of major bleeding complications in patients undergoing TAVR is associated with increased morbidity and mortality.
32553337	10	4	from	infarction	1541:1550	arg1	%					1558:1558	0.2%	1555:1558	0.2% of patients	1555:1570	In the overall cohort, stroke was observed in 1.9% and myocardial infarction in 0.2% of patients, with no significant difference between the groups (p > 0.05).
32553337	10	4	from	infarction	1541:1550	arg1	patients					1563:1570	patients	1563:1570	patients	1563:1570	In the overall cohort, stroke was observed in 1.9% and myocardial infarction in 0.2% of patients, with no significant difference between the groups (p > 0.05).
32553337	1	5	theme	bleeding	281:288	arg1	complications					290:302	bleeding complications	281:302	bleeding complications	281:302	OBJECTIVES The aim of this study was to evaluate whether protamine administration for heparin reversal after transcatheter aortic valve replacement (TAVR) reduces bleeding complications and affects patient outcomes.
32553337	8	6	theme	lower	1191:1195	arg1	rates					1197:1201	lower rates	1191:1201	lower rates of life-threatening and major bleeding in the protamine group (0.1% vs. 2.6% [p < 0.001] and 1.0% vs. 4.1% [p = 0.008], respectively)	1191:1335	This was driven mainly by lower rates of life-threatening and major bleeding in the protamine group (0.1% vs. 2.6% [p < 0.001] and 1.0% vs. 4.1% [p = 0.008], respectively).
32553337	7	7	dep	RESULTS	930:936	arg1	occurred					1043:1050	occurred	1043:1050	occurred less frequently in the protamine administration group (3.2%)	1043:1111	RESULTS The primary endpoint, a composite of 30-day all-cause mortality and life-threatening and major bleeding, occurred less frequently in the protamine administration group (3.2%) compared with the control group (8.7%) (p = 0.003).
32553337	1	8	theme	protamine	175:183	arg1	administration					185:198	protamine administration	175:198	protamine administration for heparin reversal after transcatheter aortic valve replacement (TAVR)	175:271	OBJECTIVES The aim of this study was to evaluate whether protamine administration for heparin reversal after transcatheter aortic valve replacement (TAVR) reduces bleeding complications and affects patient outcomes.
32553337	4	9	theme	covered	732:738	arg1	graft					746:750	a covered stent graft	730:750	a covered stent graft	730:750	Standard access management included the use of pre-closure devices, manual compression, and percutaneous transluminal angioplasty or implantation of a covered stent graft, if necessary.
32553337	2	10	theme	BACKGROUND	334:343	arg1	Occurrence					345:354	BACKGROUND Occurrence	334:354	BACKGROUND Occurrence of major bleeding complications in patients undergoing TAVR	334:414	BACKGROUND Occurrence of major bleeding complications in patients undergoing TAVR is associated with increased morbidity and mortality.
32553337	4	11	theme	transluminal	686:697	arg1	angioplasty					699:709	percutaneous transluminal angioplasty	673:709	percutaneous transluminal angioplasty	673:709	Standard access management included the use of pre-closure devices, manual compression, and percutaneous transluminal angioplasty or implantation of a covered stent graft, if necessary.
32553337	1	12	theme	transcatheter	227:239	arg1	replacement					254:264	transcatheter aortic valve replacement	227:264	transcatheter aortic valve replacement (TAVR)	227:271	OBJECTIVES The aim of this study was to evaluate whether protamine administration for heparin reversal after transcatheter aortic valve replacement (TAVR) reduces bleeding complications and affects patient outcomes.
32553337	1	12	theme	transcatheter	227:239	arg1	TAVR					267:270	TAVR	267:270	TAVR	267:270	OBJECTIVES The aim of this study was to evaluate whether protamine administration for heparin reversal after transcatheter aortic valve replacement (TAVR) reduces bleeding complications and affects patient outcomes.
32553337	8	13	theme	bleeding	1233:1240	arg1	rates					1197:1201	lower rates	1191:1201	lower rates of life-threatening and major bleeding in the protamine group (0.1% vs. 2.6% [p < 0.001] and 1.0% vs. 4.1% [p = 0.008], respectively)	1191:1335	This was driven mainly by lower rates of life-threatening and major bleeding in the protamine group (0.1% vs. 2.6% [p < 0.001] and 1.0% vs. 4.1% [p = 0.008], respectively).
32553337	11	14	theme	primary	1903:1909	arg1	endpoint					1911:1918	the primary endpoint	1899:1918	the primary endpoint	1899:1918	Multivariate analysis revealed that only protamine administration (odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001) and acute kidney injury (odds ratio: 5.82; 95% confidence interval: 2.02 to 16.77; p = 0.001) were independently associated with the primary endpoint.
32553337	11	15	theme	interval	1735:1742	arg1	%					1722:1722	0.24; 95%	1714:1722	odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001	1702:1767	Multivariate analysis revealed that only protamine administration (odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001) and acute kidney injury (odds ratio: 5.82; 95% confidence interval: 2.02 to 16.77; p = 0.001) were independently associated with the primary endpoint.
32553337	7	16	theme	protamine	1075:1083	arg1	group					1100:1104	the protamine administration group	1071:1104	the protamine administration group	1071:1104	RESULTS The primary endpoint, a composite of 30-day all-cause mortality and life-threatening and major bleeding, occurred less frequently in the protamine administration group (3.2%) compared with the control group (8.7%) (p = 0.003).
32553337	9	17	theme	±	1452:1452	arg1	7.8 days					1454:1461	11.1 ± 5.8 days vs. 12.7 ± 7.8 days; p = 0.05	1427:1471	7.8 days	1454:1461	Furthermore, protamine administration resulted in a significantly shorter hospital stay (11.1 ± 5.8 days vs. 12.7 ± 7.8 days; p = 0.05).
32553337	8	18	from	rates	1197:1201	arg1	group					1259:1263	the protamine group	1245:1263	the protamine group (0.1% vs. 2.6% [p < 0.001] and 1.0% vs. 4.1% [p = 0.008], respectively)	1245:1335	This was driven mainly by lower rates of life-threatening and major bleeding in the protamine group (0.1% vs. 2.6% [p < 0.001] and 1.0% vs. 4.1% [p = 0.008], respectively).
32553337	11	19	dep	administration	1686:1699	arg1	odds					1702:1705	odds	1702:1705	odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001	1702:1767	Multivariate analysis revealed that only protamine administration (odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001) and acute kidney injury (odds ratio: 5.82; 95% confidence interval: 2.02 to 16.77; p = 0.001) were independently associated with the primary endpoint.
32553337	11	20	theme	%	1815:1815	arg1	interval					1828:1835	95% confidence interval	1813:1835	odds ratio: 5.82; 95% confidence interval: 2.02 to 16.77; p = 0.001	1795:1861	Multivariate analysis revealed that only protamine administration (odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001) and acute kidney injury (odds ratio: 5.82; 95% confidence interval: 2.02 to 16.77; p = 0.001) were independently associated with the primary endpoint.
32553337	8	21	from	group	1259:1263	arg1	life-threatening					1206:1221	life-threatening	1206:1221	life-threatening	1206:1221	This was driven mainly by lower rates of life-threatening and major bleeding in the protamine group (0.1% vs. 2.6% [p < 0.001] and 1.0% vs. 4.1% [p = 0.008], respectively).
32553337	8	21	from	group	1259:1263	arg1	rates					1197:1201	lower rates	1191:1201	lower rates of life-threatening and major bleeding in the protamine group (0.1% vs. 2.6% [p < 0.001] and 1.0% vs. 4.1% [p = 0.008], respectively)	1191:1335	This was driven mainly by lower rates of life-threatening and major bleeding in the protamine group (0.1% vs. 2.6% [p < 0.001] and 1.0% vs. 4.1% [p = 0.008], respectively).
32553337	9	22	dep	hospital	1412:1419	arg1	7.8 days					1454:1461	11.1 ± 5.8 days vs. 12.7 ± 7.8 days; p = 0.05	1427:1471	7.8 days	1454:1461	Furthermore, protamine administration resulted in a significantly shorter hospital stay (11.1 ± 5.8 days vs. 12.7 ± 7.8 days; p = 0.05).
32553337	9	22	dep	hospital	1412:1419	arg1	stay					1421:1424	stay	1421:1424	stay	1421:1424	Furthermore, protamine administration resulted in a significantly shorter hospital stay (11.1 ± 5.8 days vs. 12.7 ± 7.8 days; p = 0.05).
32553337	9	22	dep	hospital	1412:1419	arg1	5.8 days					1434:1441	11.1 ± 5.8 days vs. 12.7 ± 7.8 days; p = 0.05	1427:1471	5.8 days	1434:1441	Furthermore, protamine administration resulted in a significantly shorter hospital stay (11.1 ± 5.8 days vs. 12.7 ± 7.8 days; p = 0.05).
32553337	11	23	theme	only	1671:1674	arg1	administration					1686:1699	only protamine administration (odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001)	1671:1768	only protamine administration (odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001)	1671:1768	Multivariate analysis revealed that only protamine administration (odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001) and acute kidney injury (odds ratio: 5.82; 95% confidence interval: 2.02 to 16.77; p = 0.001) were independently associated with the primary endpoint.
32553337	11	24	dep	odds	1702:1705	arg1	0.58					1753:1756	0.58	1753:1756	0.58	1753:1756	Multivariate analysis revealed that only protamine administration (odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001) and acute kidney injury (odds ratio: 5.82; 95% confidence interval: 2.02 to 16.77; p = 0.001) were independently associated with the primary endpoint.
32553337	11	24	dep	odds	1702:1705	arg1	%					1722:1722	0.24; 95%	1714:1722	odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001	1702:1767	Multivariate analysis revealed that only protamine administration (odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001) and acute kidney injury (odds ratio: 5.82; 95% confidence interval: 2.02 to 16.77; p = 0.001) were independently associated with the primary endpoint.
32553337	11	24	dep	odds	1702:1705	arg1	p = 0.001					1759:1767	p = 0.001	1759:1767	p = 0.001	1759:1767	Multivariate analysis revealed that only protamine administration (odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001) and acute kidney injury (odds ratio: 5.82; 95% confidence interval: 2.02 to 16.77; p = 0.001) were independently associated with the primary endpoint.
32553337	11	24	dep	odds	1702:1705	arg1	ratio					1707:1711	ratio	1707:1711	odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001	1702:1767	Multivariate analysis revealed that only protamine administration (odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001) and acute kidney injury (odds ratio: 5.82; 95% confidence interval: 2.02 to 16.77; p = 0.001) were independently associated with the primary endpoint.
32553337	4	25	theme	graft	746:750	arg1	devices					640:646	pre-closure devices	628:646	pre-closure devices	628:646	Standard access management included the use of pre-closure devices, manual compression, and percutaneous transluminal angioplasty or implantation of a covered stent graft, if necessary.
32553337	4	25	theme	graft	746:750	arg1	compression					656:666	manual compression	649:666	manual compression	649:666	Standard access management included the use of pre-closure devices, manual compression, and percutaneous transluminal angioplasty or implantation of a covered stent graft, if necessary.
32553337	4	25	theme	graft	746:750	arg1	angioplasty					699:709	percutaneous transluminal angioplasty	673:709	percutaneous transluminal angioplasty	673:709	Standard access management included the use of pre-closure devices, manual compression, and percutaneous transluminal angioplasty or implantation of a covered stent graft, if necessary.
32553337	4	25	theme	graft	746:750	arg1	implantation					714:725	implantation	714:725	implantation	714:725	Standard access management included the use of pre-closure devices, manual compression, and percutaneous transluminal angioplasty or implantation of a covered stent graft, if necessary.
32553337	12	26	theme	CONCLUSIONS	1921:1931	arg1	administration					1943:1956	CONCLUSIONS Protamine administration	1921:1956	CONCLUSIONS Protamine administration	1921:1956	CONCLUSIONS Protamine administration resulted in significantly lower rates of life-threatening and major bleeding complications compared with patients without heparin reversal.
32553337	2	27	from	Occurrence	345:354	arg1	patients					391:398	patients	391:398	patients undergoing TAVR	391:414	BACKGROUND Occurrence of major bleeding complications in patients undergoing TAVR is associated with increased morbidity and mortality.
32553337	10	28	located	observed	1509:1516	arg1	infarction					1541:1550	myocardial infarction	1530:1550	myocardial infarction in 0.2% of patients	1530:1570	In the overall cohort, stroke was observed in 1.9% and myocardial infarction in 0.2% of patients, with no significant difference between the groups (p > 0.05).
32553337	10	28	located	observed	1509:1516	arg1	cohort					1490:1495	the overall cohort	1478:1495	the overall cohort	1478:1495	In the overall cohort, stroke was observed in 1.9% and myocardial infarction in 0.2% of patients, with no significant difference between the groups (p > 0.05).
32553337	10	28	located	observed	1509:1516	arg1	%					1524:1524	1.9%	1521:1524	1.9%	1521:1524	In the overall cohort, stroke was observed in 1.9% and myocardial infarction in 0.2% of patients, with no significant difference between the groups (p > 0.05).
32553337	10	28	located	observed	1509:1516	arg2	stroke					1498:1503	stroke	1498:1503	stroke	1498:1503	In the overall cohort, stroke was observed in 1.9% and myocardial infarction in 0.2% of patients, with no significant difference between the groups (p > 0.05).
32553337	10	29	theme	significant	1581:1591	arg1	difference					1593:1602	no significant difference	1578:1602	no significant difference between the groups (p > 0.05)	1578:1632	In the overall cohort, stroke was observed in 1.9% and myocardial infarction in 0.2% of patients, with no significant difference between the groups (p > 0.05).
32553337	5	30	theme	Clinical	796:803	arg1	guidelines					814:823	Good Clinical Practice guidelines	791:823	Good Clinical Practice guidelines	791:823	The study complied with Good Clinical Practice guidelines and was approved by the local ethics committee.
32553337	7	31	theme	mortality	992:1000	arg1	mortality					992:1000	30-day all-cause mortality	975:1000	30-day all-cause mortality	975:1000	RESULTS The primary endpoint, a composite of 30-day all-cause mortality and life-threatening and major bleeding, occurred less frequently in the protamine administration group (3.2%) compared with the control group (8.7%) (p = 0.003).
32553337	7	31	theme	mortality	992:1000	arg1	endpoint					950:957	The primary endpoint	938:957	The primary endpoint	938:957	RESULTS The primary endpoint, a composite of 30-day all-cause mortality and life-threatening and major bleeding, occurred less frequently in the protamine administration group (3.2%) compared with the control group (8.7%) (p = 0.003).
32553337	7	31	theme	mortality	992:1000	arg1	composite					962:970	a composite	960:970	a composite of 30-day all-cause mortality	960:1000	RESULTS The primary endpoint, a composite of 30-day all-cause mortality and life-threatening and major bleeding, occurred less frequently in the protamine administration group (3.2%) compared with the control group (8.7%) (p = 0.003).
32553337	0	32	theme	Bleeding Complications	66:87	arg1	Prevention					52:61	Prevention	52:61	Prevention of Bleeding Complications in Patients Undergoing TAVR	52:115	Safety and Efficacy of Protamine Administration for Prevention of Bleeding Complications in Patients Undergoing TAVR.
32553337	11	33	theme	kidney	1780:1785	arg1	injury					1787:1792	acute kidney injury	1774:1792	acute kidney injury (odds ratio: 5.82; 95% confidence interval: 2.02 to 16.77; p = 0.001)	1774:1862	Multivariate analysis revealed that only protamine administration (odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001) and acute kidney injury (odds ratio: 5.82; 95% confidence interval: 2.02 to 16.77; p = 0.001) were independently associated with the primary endpoint.
32553337	13	34	theme	myocardial	2123:2132	arg1	infarction					2134:2143	myocardial infarction	2123:2143	myocardial infarction	2123:2143	Occurrence of stroke and myocardial infarction was not increased by protamine administration.
32553337	7	35	theme	30-day	975:980	arg1	mortality					992:1000	30-day all-cause mortality	975:1000	30-day all-cause mortality	975:1000	RESULTS The primary endpoint, a composite of 30-day all-cause mortality and life-threatening and major bleeding, occurred less frequently in the protamine administration group (3.2%) compared with the control group (8.7%) (p = 0.003).
32553337	7	36	dep	occurred	1043:1050	arg1	%					1110:1110	3.2%	1107:1110	3.2%	1107:1110	RESULTS The primary endpoint, a composite of 30-day all-cause mortality and life-threatening and major bleeding, occurred less frequently in the protamine administration group (3.2%) compared with the control group (8.7%) (p = 0.003).
32553337	12	37	theme	heparin	2080:2086	arg1	reversal					2088:2095	heparin reversal	2080:2095	heparin reversal	2080:2095	CONCLUSIONS Protamine administration resulted in significantly lower rates of life-threatening and major bleeding complications compared with patients without heparin reversal.
32553337	0	38	theme	Protamine	23:31	arg1	Administration					33:46	Protamine Administration	23:46	Protamine Administration	23:46	Safety and Efficacy of Protamine Administration for Prevention of Bleeding Complications in Patients Undergoing TAVR.
32553337	11	39	dep	injury	1787:1792	arg1	ratio					1800:1804	odds ratio	1795:1804	odds ratio: 5.82; 95% confidence interval: 2.02 to 16.77; p = 0.001	1795:1861	Multivariate analysis revealed that only protamine administration (odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001) and acute kidney injury (odds ratio: 5.82; 95% confidence interval: 2.02 to 16.77; p = 0.001) were independently associated with the primary endpoint.
32553337	10	40	theme	overall	1482:1488	arg1	cohort					1490:1495	the overall cohort	1478:1495	the overall cohort	1478:1495	In the overall cohort, stroke was observed in 1.9% and myocardial infarction in 0.2% of patients, with no significant difference between the groups (p > 0.05).
32553337	2	41	theme	complications	374:386	arg1	Occurrence					345:354	BACKGROUND Occurrence	334:354	BACKGROUND Occurrence of major bleeding complications in patients undergoing TAVR	334:414	BACKGROUND Occurrence of major bleeding complications in patients undergoing TAVR is associated with increased morbidity and mortality.
32553337	5	42	theme	local	849:853	arg1	committee					862:870	the local ethics committee	845:870	the local ethics committee	845:870	The study complied with Good Clinical Practice guidelines and was approved by the local ethics committee.
32553337	13	43	theme	stroke	2112:2117	arg1	Occurrence					2098:2107	Occurrence	2098:2107	Occurrence of stroke and myocardial infarction	2098:2143	Occurrence of stroke and myocardial infarction was not increased by protamine administration.
32553337	4	44	theme	Standard	581:588	arg1	management					597:606	Standard access management	581:606	Standard access management	581:606	Standard access management included the use of pre-closure devices, manual compression, and percutaneous transluminal angioplasty or implantation of a covered stent graft, if necessary.
32553337	10	45	theme	p	1624:1624	arg1	>					1626:1626	p > 0.05	1624:1631	p > 0.05	1624:1631	In the overall cohort, stroke was observed in 1.9% and myocardial infarction in 0.2% of patients, with no significant difference between the groups (p > 0.05).
32553337	10	45	theme	p	1624:1624	arg1	groups					1616:1621	the groups	1612:1621	the groups (p > 0.05)	1612:1632	In the overall cohort, stroke was observed in 1.9% and myocardial infarction in 0.2% of patients, with no significant difference between the groups (p > 0.05).
32553337	9	46	theme	protamine	1351:1359	arg1	administration					1361:1374	protamine administration	1351:1374	protamine administration	1351:1374	Furthermore, protamine administration resulted in a significantly shorter hospital stay (11.1 ± 5.8 days vs. 12.7 ± 7.8 days; p = 0.05).
32553337	10	47	from	%	1524:1524	arg1	%					1558:1558	0.2%	1555:1558	0.2% of patients	1555:1570	In the overall cohort, stroke was observed in 1.9% and myocardial infarction in 0.2% of patients, with no significant difference between the groups (p > 0.05).
32553337	10	47	from	%	1524:1524	arg1	patients					1563:1570	patients	1563:1570	patients	1563:1570	In the overall cohort, stroke was observed in 1.9% and myocardial infarction in 0.2% of patients, with no significant difference between the groups (p > 0.05).
32553337	1	48	dep	OBJECTIVES	118:127	arg1	was					151:153	was	151:153	was to evaluate whether protamine administration for heparin reversal after transcatheter aortic valve replacement (TAVR) reduces bleeding complications and affects patient outcomes	151:331	OBJECTIVES The aim of this study was to evaluate whether protamine administration for heparin reversal after transcatheter aortic valve replacement (TAVR) reduces bleeding complications and affects patient outcomes.
32553337	4	49	theme	manual	649:654	arg1	compression					656:666	manual compression	649:666	manual compression	649:666	Standard access management included the use of pre-closure devices, manual compression, and percutaneous transluminal angioplasty or implantation of a covered stent graft, if necessary.
32553337	11	50	dep	16.77	1846:1850	arg1	to					1843:1844	to	1843:1844	to	1843:1844	Multivariate analysis revealed that only protamine administration (odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001) and acute kidney injury (odds ratio: 5.82; 95% confidence interval: 2.02 to 16.77; p = 0.001) were independently associated with the primary endpoint.
32553337	12	51	theme	life-threatening	1999:2014	arg1	complications					2035:2047	life-threatening and major bleeding complications	1999:2047	complications	2035:2047	CONCLUSIONS Protamine administration resulted in significantly lower rates of life-threatening and major bleeding complications compared with patients without heparin reversal.
32553337	9	52	theme	shorter	1404:1410	arg1	hospital					1412:1419	a significantly shorter hospital	1388:1419	a significantly shorter hospital stay (11.1 ± 5.8 days vs. 12.7 ± 7.8 days; p = 0.05)	1388:1472	Furthermore, protamine administration resulted in a significantly shorter hospital stay (11.1 ± 5.8 days vs. 12.7 ± 7.8 days; p = 0.05).
32553337	4	53	theme	devices	640:646	arg1	use					621:623	the use	617:623	the use of pre-closure devices, manual compression, and percutaneous transluminal angioplasty or implantation of a covered stent graft	617:750	Standard access management included the use of pre-closure devices, manual compression, and percutaneous transluminal angioplasty or implantation of a covered stent graft, if necessary.
32553337	12	54	theme	bleeding	2026:2033	arg1	complications					2035:2047	life-threatening and major bleeding complications	1999:2047	complications	2035:2047	CONCLUSIONS Protamine administration resulted in significantly lower rates of life-threatening and major bleeding complications compared with patients without heparin reversal.
32553337	8	55	theme	protamine	1249:1257	arg1	group					1259:1263	the protamine group	1245:1263	the protamine group (0.1% vs. 2.6% [p < 0.001] and 1.0% vs. 4.1% [p = 0.008], respectively)	1245:1335	This was driven mainly by lower rates of life-threatening and major bleeding in the protamine group (0.1% vs. 2.6% [p < 0.001] and 1.0% vs. 4.1% [p = 0.008], respectively).
32553337	7	56	theme	control	1131:1137	arg1	group					1139:1143	the control group	1127:1143	the control group (8.7%) (p = 0.003)	1127:1162	RESULTS The primary endpoint, a composite of 30-day all-cause mortality and life-threatening and major bleeding, occurred less frequently in the protamine administration group (3.2%) compared with the control group (8.7%) (p = 0.003).
32553337	7	56	theme	control	1131:1137	arg1	%					1149:1149	8.7%	1146:1149	8.7%	1146:1149	RESULTS The primary endpoint, a composite of 30-day all-cause mortality and life-threatening and major bleeding, occurred less frequently in the protamine administration group (3.2%) compared with the control group (8.7%) (p = 0.003).
32553337	7	56	theme	control	1131:1137	arg1	p = 0.003					1153:1161	p = 0.003	1153:1161	p = 0.003	1153:1161	RESULTS The primary endpoint, a composite of 30-day all-cause mortality and life-threatening and major bleeding, occurred less frequently in the protamine administration group (3.2%) compared with the control group (8.7%) (p = 0.003).
32553337	12	57	theme	major	2020:2024	arg1	complications					2035:2047	life-threatening and major bleeding complications	1999:2047	complications	2035:2047	CONCLUSIONS Protamine administration resulted in significantly lower rates of life-threatening and major bleeding complications compared with patients without heparin reversal.
32553337	10	58	theme	myocardial	1530:1539	arg1	infarction					1541:1550	myocardial infarction	1530:1550	myocardial infarction in 0.2% of patients	1530:1570	In the overall cohort, stroke was observed in 1.9% and myocardial infarction in 0.2% of patients, with no significant difference between the groups (p > 0.05).
32553337	1	59	theme	aortic	241:246	arg1	replacement					254:264	transcatheter aortic valve replacement	227:264	transcatheter aortic valve replacement (TAVR)	227:271	OBJECTIVES The aim of this study was to evaluate whether protamine administration for heparin reversal after transcatheter aortic valve replacement (TAVR) reduces bleeding complications and affects patient outcomes.
32553337	1	59	theme	aortic	241:246	arg1	TAVR					267:270	TAVR	267:270	TAVR	267:270	OBJECTIVES The aim of this study was to evaluate whether protamine administration for heparin reversal after transcatheter aortic valve replacement (TAVR) reduces bleeding complications and affects patient outcomes.
32553337	1	60	theme	heparin	204:210	arg1	reversal					212:219	heparin reversal	204:219	heparin reversal	204:219	OBJECTIVES The aim of this study was to evaluate whether protamine administration for heparin reversal after transcatheter aortic valve replacement (TAVR) reduces bleeding complications and affects patient outcomes.
32553337	8	61	theme	life-threatening	1206:1221	arg1	rates					1197:1201	lower rates	1191:1201	lower rates of life-threatening and major bleeding in the protamine group (0.1% vs. 2.6% [p < 0.001] and 1.0% vs. 4.1% [p = 0.008], respectively)	1191:1335	This was driven mainly by lower rates of life-threatening and major bleeding in the protamine group (0.1% vs. 2.6% [p < 0.001] and 1.0% vs. 4.1% [p = 0.008], respectively).
32553337	4	62	theme	percutaneous	673:684	arg1	angioplasty					699:709	percutaneous transluminal angioplasty	673:709	percutaneous transluminal angioplasty	673:709	Standard access management included the use of pre-closure devices, manual compression, and percutaneous transluminal angioplasty or implantation of a covered stent graft, if necessary.
32553337	10	63	theme	patients	1563:1570	arg1	%					1558:1558	0.2%	1555:1558	0.2% of patients	1555:1570	In the overall cohort, stroke was observed in 1.9% and myocardial infarction in 0.2% of patients, with no significant difference between the groups (p > 0.05).
32553337	10	63	theme	patients	1563:1570	arg1	patients					1563:1570	patients	1563:1570	patients	1563:1570	In the overall cohort, stroke was observed in 1.9% and myocardial infarction in 0.2% of patients, with no significant difference between the groups (p > 0.05).
32553337	11	64	theme	confidence	1817:1826	arg1	interval					1828:1835	95% confidence interval	1813:1835	odds ratio: 5.82; 95% confidence interval: 2.02 to 16.77; p = 0.001	1795:1861	Multivariate analysis revealed that only protamine administration (odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001) and acute kidney injury (odds ratio: 5.82; 95% confidence interval: 2.02 to 16.77; p = 0.001) were independently associated with the primary endpoint.
32553337	4	65	theme	angioplasty	699:709	arg1	use					621:623	the use	617:623	the use of pre-closure devices, manual compression, and percutaneous transluminal angioplasty or implantation of a covered stent graft	617:750	Standard access management included the use of pre-closure devices, manual compression, and percutaneous transluminal angioplasty or implantation of a covered stent graft, if necessary.
32553337	7	66	theme	administration	1085:1098	arg1	group					1100:1104	the protamine administration group	1071:1104	the protamine administration group	1071:1104	RESULTS The primary endpoint, a composite of 30-day all-cause mortality and life-threatening and major bleeding, occurred less frequently in the protamine administration group (3.2%) compared with the control group (8.7%) (p = 0.003).
32553337	12	67	theme	Protamine	1933:1941	arg1	administration					1943:1956	CONCLUSIONS Protamine administration	1921:1956	CONCLUSIONS Protamine administration	1921:1956	CONCLUSIONS Protamine administration resulted in significantly lower rates of life-threatening and major bleeding complications compared with patients without heparin reversal.
32553337	11	68	theme	protamine	1676:1684	arg1	administration					1686:1699	only protamine administration (odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001)	1671:1768	only protamine administration (odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001)	1671:1768	Multivariate analysis revealed that only protamine administration (odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001) and acute kidney injury (odds ratio: 5.82; 95% confidence interval: 2.02 to 16.77; p = 0.001) were independently associated with the primary endpoint.
32553337	5	69	theme	Practice	805:812	arg1	guidelines					814:823	Good Clinical Practice guidelines	791:823	Good Clinical Practice guidelines	791:823	The study complied with Good Clinical Practice guidelines and was approved by the local ethics committee.
32553337	12	70	theme	lower	1984:1988	arg1	rates					1990:1994	significantly lower rates	1970:1994	significantly lower rates of life-threatening and major bleeding complications compared with patients without heparin reversal	1970:2095	CONCLUSIONS Protamine administration resulted in significantly lower rates of life-threatening and major bleeding complications compared with patients without heparin reversal.
32553337	1	71	theme	study	145:149	arg1	aim					133:135	The aim	129:135	The aim of this study	129:149	OBJECTIVES The aim of this study was to evaluate whether protamine administration for heparin reversal after transcatheter aortic valve replacement (TAVR) reduces bleeding complications and affects patient outcomes.
32553337	4	72	theme	stent	740:744	arg1	graft					746:750	a covered stent graft	730:750	a covered stent graft	730:750	Standard access management included the use of pre-closure devices, manual compression, and percutaneous transluminal angioplasty or implantation of a covered stent graft, if necessary.
32553337	13	73	theme	protamine	2166:2174	arg1	administration					2176:2189	protamine administration	2166:2189	protamine administration	2166:2189	Occurrence of stroke and myocardial infarction was not increased by protamine administration.
32553337	0	74	from	Prevention	52:61	arg1	Patients					92:99	Patients	92:99	Patients Undergoing TAVR	92:115	Safety and Efficacy of Protamine Administration for Prevention of Bleeding Complications in Patients Undergoing TAVR.
32553337	5	75	theme	Good	791:794	arg1	guidelines					814:823	Good Clinical Practice guidelines	791:823	Good Clinical Practice guidelines	791:823	The study complied with Good Clinical Practice guidelines and was approved by the local ethics committee.
32553337	11	76	dep	0.58	1753:1756	arg1	to					1750:1751	to	1750:1751	to	1750:1751	Multivariate analysis revealed that only protamine administration (odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001) and acute kidney injury (odds ratio: 5.82; 95% confidence interval: 2.02 to 16.77; p = 0.001) were independently associated with the primary endpoint.
32553337	11	77	theme	odds	1795:1798	arg1	ratio					1800:1804	odds ratio	1795:1804	odds ratio: 5.82; 95% confidence interval: 2.02 to 16.77; p = 0.001	1795:1861	Multivariate analysis revealed that only protamine administration (odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001) and acute kidney injury (odds ratio: 5.82; 95% confidence interval: 2.02 to 16.77; p = 0.001) were independently associated with the primary endpoint.
32553337	5	78	theme	ethics	855:860	arg1	committee					862:870	the local ethics committee	845:870	the local ethics committee	845:870	The study complied with Good Clinical Practice guidelines and was approved by the local ethics committee.
32553337	13	79	theme	infarction	2134:2143	arg1	Occurrence					2098:2107	Occurrence	2098:2107	Occurrence of stroke and myocardial infarction	2098:2143	Occurrence of stroke and myocardial infarction was not increased by protamine administration.
32553337	7	80	theme	all-cause	982:990	arg1	mortality					992:1000	30-day all-cause mortality	975:1000	30-day all-cause mortality	975:1000	RESULTS The primary endpoint, a composite of 30-day all-cause mortality and life-threatening and major bleeding, occurred less frequently in the protamine administration group (3.2%) compared with the control group (8.7%) (p = 0.003).
32553337	8	81	dep	group	1259:1263	arg1	%					1308:1308	1.0% vs. 4.1%	1296:1308	1.0% vs. 4.1% [p = 0.008]	1296:1320	This was driven mainly by lower rates of life-threatening and major bleeding in the protamine group (0.1% vs. 2.6% [p < 0.001] and 1.0% vs. 4.1% [p = 0.008], respectively).
32553337	8	81	dep	group	1259:1263	arg1	%					1269:1269	0.1%	1266:1269	0.1%	1266:1269	This was driven mainly by lower rates of life-threatening and major bleeding in the protamine group (0.1% vs. 2.6% [p < 0.001] and 1.0% vs. 4.1% [p = 0.008], respectively).
32553337	8	81	dep	group	1259:1263	arg1	%					1278:1278	2.6%	1275:1278	2.6%	1275:1278	This was driven mainly by lower rates of life-threatening and major bleeding in the protamine group (0.1% vs. 2.6% [p < 0.001] and 1.0% vs. 4.1% [p = 0.008], respectively).
32553337	0	82	theme	Administration	33:46	arg1	Efficacy					11:18	Efficacy	11:18	Efficacy	11:18	Safety and Efficacy of Protamine Administration for Prevention of Bleeding Complications in Patients Undergoing TAVR.
32553337	0	82	theme	Administration	33:46	arg1	Safety					0:5	Safety	0:5	Safety	0:5	Safety and Efficacy of Protamine Administration for Prevention of Bleeding Complications in Patients Undergoing TAVR.
32553337	1	83	theme	patient	316:322	arg1	outcomes					324:331	patient outcomes	316:331	patient outcomes	316:331	OBJECTIVES The aim of this study was to evaluate whether protamine administration for heparin reversal after transcatheter aortic valve replacement (TAVR) reduces bleeding complications and affects patient outcomes.
32553337	3	84	dep	METHODS	470:476	arg1	included					489:496	included	489:496	included 873 patients undergoing TAVR, of whom 677 received protamine for heparin reversal.	489:579	METHODS This study included 873 patients undergoing TAVR, of whom 677 received protamine for heparin reversal.
32553337	2	85	theme	increased	435:443	arg1	morbidity					445:453	morbidity	445:453	morbidity	445:453	BACKGROUND Occurrence of major bleeding complications in patients undergoing TAVR is associated with increased morbidity and mortality.
32553337	4	86	theme	access	590:595	arg1	management					597:606	Standard access management	581:606	Standard access management	581:606	Standard access management included the use of pre-closure devices, manual compression, and percutaneous transluminal angioplasty or implantation of a covered stent graft, if necessary.
32553337	11	87	theme	Multivariate	1635:1646	arg1	analysis					1648:1655	Multivariate analysis	1635:1655	Multivariate analysis	1635:1655	Multivariate analysis revealed that only protamine administration (odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001) and acute kidney injury (odds ratio: 5.82; 95% confidence interval: 2.02 to 16.77; p = 0.001) were independently associated with the primary endpoint.
32553337	2	88	theme	bleeding	365:372	arg1	complications					374:386	major bleeding complications	359:386	major bleeding complications	359:386	BACKGROUND Occurrence of major bleeding complications in patients undergoing TAVR is associated with increased morbidity and mortality.
32553337	11	89	dep	ratio	1800:1804	arg1	interval					1828:1835	95% confidence interval	1813:1835	odds ratio: 5.82; 95% confidence interval: 2.02 to 16.77; p = 0.001	1795:1861	Multivariate analysis revealed that only protamine administration (odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001) and acute kidney injury (odds ratio: 5.82; 95% confidence interval: 2.02 to 16.77; p = 0.001) were independently associated with the primary endpoint.
32553337	11	89	dep	ratio	1800:1804	arg1	5.82					1807:1810	5.82	1807:1810	5.82	1807:1810	Multivariate analysis revealed that only protamine administration (odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001) and acute kidney injury (odds ratio: 5.82; 95% confidence interval: 2.02 to 16.77; p = 0.001) were independently associated with the primary endpoint.
32553337	11	89	dep	ratio	1800:1804	arg1	p = 0.001					1853:1861	p = 0.001	1853:1861	p = 0.001	1853:1861	Multivariate analysis revealed that only protamine administration (odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001) and acute kidney injury (odds ratio: 5.82; 95% confidence interval: 2.02 to 16.77; p = 0.001) were independently associated with the primary endpoint.
32553337	11	89	dep	ratio	1800:1804	arg1	16.77					1846:1850	16.77	1846:1850	16.77	1846:1850	Multivariate analysis revealed that only protamine administration (odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001) and acute kidney injury (odds ratio: 5.82; 95% confidence interval: 2.02 to 16.77; p = 0.001) were independently associated with the primary endpoint.
32553337	4	90	theme	compression	656:666	arg1	use					621:623	the use	617:623	the use of pre-closure devices, manual compression, and percutaneous transluminal angioplasty or implantation of a covered stent graft	617:750	Standard access management included the use of pre-closure devices, manual compression, and percutaneous transluminal angioplasty or implantation of a covered stent graft, if necessary.
32553337	12	91	theme	complications	2035:2047	arg1	rates					1990:1994	significantly lower rates	1970:1994	significantly lower rates of life-threatening and major bleeding complications compared with patients without heparin reversal	1970:2095	CONCLUSIONS Protamine administration resulted in significantly lower rates of life-threatening and major bleeding complications compared with patients without heparin reversal.
32553337	3	92	theme	heparin	563:569	arg1	reversal					571:578	heparin reversal	563:578	heparin reversal	563:578	METHODS This study included 873 patients undergoing TAVR, of whom 677 received protamine for heparin reversal.
32553337	1	93	theme	valve	248:252	arg1	replacement					254:264	transcatheter aortic valve replacement	227:264	transcatheter aortic valve replacement (TAVR)	227:271	OBJECTIVES The aim of this study was to evaluate whether protamine administration for heparin reversal after transcatheter aortic valve replacement (TAVR) reduces bleeding complications and affects patient outcomes.
32553337	1	93	theme	valve	248:252	arg1	TAVR					267:270	TAVR	267:270	TAVR	267:270	OBJECTIVES The aim of this study was to evaluate whether protamine administration for heparin reversal after transcatheter aortic valve replacement (TAVR) reduces bleeding complications and affects patient outcomes.
32553337	6	94	theme	informed	881:888	arg1	consent					890:896	Written informed consent	873:896	Written informed consent	873:896	Written informed consent was obtained from all patients.
32553337	11	95	theme	acute	1774:1778	arg1	injury					1787:1792	acute kidney injury	1774:1792	acute kidney injury (odds ratio: 5.82; 95% confidence interval: 2.02 to 16.77; p = 0.001)	1774:1862	Multivariate analysis revealed that only protamine administration (odds ratio: 0.24; 95% confidence interval: 0.10 to 0.58; p = 0.001) and acute kidney injury (odds ratio: 5.82; 95% confidence interval: 2.02 to 16.77; p = 0.001) were independently associated with the primary endpoint.
32553337	7	96	theme	primary	942:948	arg1	mortality					992:1000	30-day all-cause mortality	975:1000	30-day all-cause mortality	975:1000	RESULTS The primary endpoint, a composite of 30-day all-cause mortality and life-threatening and major bleeding, occurred less frequently in the protamine administration group (3.2%) compared with the control group (8.7%) (p = 0.003).
32553337	7	96	theme	primary	942:948	arg1	endpoint					950:957	The primary endpoint	938:957	The primary endpoint	938:957	RESULTS The primary endpoint, a composite of 30-day all-cause mortality and life-threatening and major bleeding, occurred less frequently in the protamine administration group (3.2%) compared with the control group (8.7%) (p = 0.003).
32553337	7	96	theme	primary	942:948	arg1	composite					962:970	a composite	960:970	a composite of 30-day all-cause mortality	960:1000	RESULTS The primary endpoint, a composite of 30-day all-cause mortality and life-threatening and major bleeding, occurred less frequently in the protamine administration group (3.2%) compared with the control group (8.7%) (p = 0.003).
32553337	7	96	theme	primary	942:948	arg1	life-threatening					1006:1021	life-threatening	1006:1021	life-threatening	1006:1021	RESULTS The primary endpoint, a composite of 30-day all-cause mortality and life-threatening and major bleeding, occurred less frequently in the protamine administration group (3.2%) compared with the control group (8.7%) (p = 0.003).
32553337	7	96	theme	primary	942:948	arg1	bleeding					1033:1040	bleeding	1033:1040	bleeding	1033:1040	RESULTS The primary endpoint, a composite of 30-day all-cause mortality and life-threatening and major bleeding, occurred less frequently in the protamine administration group (3.2%) compared with the control group (8.7%) (p = 0.003).
33669956	3	0	with	synthesis	503:511	arg1	saliva					454:459	saliva	454:459	saliva	454:459	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	3	1	theme	UDMA	677:680	arg1	absence					666:672	absence	666:672	absence	666:672	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	3	1	theme	UDMA	677:680	arg1	presence					653:660	the presence	649:660	the presence	649:660	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	3	2	with	transport	520:528	arg1	saliva					454:459	saliva	454:459	saliva	454:459	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	3	3	with	tolerances	583:592	arg1	saliva					454:459	saliva	454:459	saliva	454:459	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	7	4	theme	cells	1355:1359	arg1	interactions					1329:1340	the interactions	1325:1340	the interactions of bacterial cells with saliva	1325:1371	Notably, UDMA did not significantly alter the interactions of bacterial cells with saliva.
33669956	6	5	theme	intracellular	1199:1211	arg1	polysaccharides					1213:1227	intracellular polysaccharides	1199:1227	intracellular polysaccharides	1199:1227	UDMA did not significantly affect the viability or planktonic growth of cells, but diminished the ability of S. mutans to metabolize carbohydrates and thus maintain the level of intracellular polysaccharides, although the tendency for sugar transport increased.
33669956	5	6	theme	acid	890:893	arg1	tolerance					895:903	the acid tolerance	886:903	the acid tolerance of S. mutans	886:916	Furthermore, UDMA reduced the acid tolerance of S. mutans, but enhanced its tolerance to oxidative stress, thus favoring the early stage of biofilm development.
33669956	3	7	theme	oxidative-	556:565	arg1	tolerances					583:592	oxidative- and acid-stress tolerances	556:592	oxidative- and acid-stress tolerances of S. mutans	556:605	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	2	8	theme	resins	328:333	arg1	component					298:306	a common monomer component	281:306	a common monomer component of dental composite resins	281:333	This study assessed the biological effects of urethane dimethacrylate (UDMA), a common monomer component of dental composite resins, on the cariogenic properties of Streptococcus mutans.
33669956	2	8	theme	resins	328:333	arg1	dimethacrylate					258:271	urethane dimethacrylate	249:271	urethane dimethacrylate (UDMA)	249:278	This study assessed the biological effects of urethane dimethacrylate (UDMA), a common monomer component of dental composite resins, on the cariogenic properties of Streptococcus mutans.
33669956	3	9	with	rate	412:415	arg1	saliva					454:459	saliva	454:459	saliva	454:459	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	6	10	theme	sugar	1256:1260	arg1	transport					1262:1270	sugar transport	1256:1270	sugar transport	1256:1270	UDMA did not significantly affect the viability or planktonic growth of cells, but diminished the ability of S. mutans to metabolize carbohydrates and thus maintain the level of intracellular polysaccharides, although the tendency for sugar transport increased.
33669956	2	11	theme	monomer	290:296	arg1	component					298:306	a common monomer component	281:306	a common monomer component of dental composite resins	281:333	This study assessed the biological effects of urethane dimethacrylate (UDMA), a common monomer component of dental composite resins, on the cariogenic properties of Streptococcus mutans.
33669956	2	11	theme	monomer	290:296	arg1	dimethacrylate					258:271	urethane dimethacrylate	249:271	urethane dimethacrylate (UDMA)	249:278	This study assessed the biological effects of urethane dimethacrylate (UDMA), a common monomer component of dental composite resins, on the cariogenic properties of Streptococcus mutans.
33669956	3	12	from	Changes	390:396	arg1	formation					426:434	biofilm formation	418:434	biofilm formation	418:434	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	3	12	from	Changes	390:396	arg1	transport					520:528	sugar transport	514:528	sugar transport	514:528	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	3	12	from	Changes	390:396	arg1	interaction					437:447	interaction	437:447	interaction with saliva	437:459	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	3	12	from	Changes	390:396	arg1	adhesion					486:493	adhesion	486:493	adhesion	486:493	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	3	12	from	Changes	390:396	arg1	rate					412:415	the growth rate	401:415	the growth rate	401:415	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	3	12	from	Changes	390:396	arg1	profiles					542:549	glycolytic profiles	531:549	glycolytic profiles	531:549	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	3	12	from	Changes	390:396	arg1	tolerances					583:592	oxidative- and acid-stress tolerances	556:592	oxidative- and acid-stress tolerances of S. mutans	556:605	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	3	12	from	Changes	390:396	arg1	synthesis					503:511	glucan synthesis	496:511	glucan synthesis	496:511	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	3	12	from	Changes	390:396	arg1	hydrophobicity					470:483	surface hydrophobicity	462:483	surface hydrophobicity	462:483	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	4	13	theme	biofilm	841:847	arg1	formation					849:857	enhanced biofilm formation	832:857	enhanced biofilm formation	832:857	The results indicated that UDMA promotes the adhesion of S. mutans to the underlying surfaces and extracellular polysaccharide synthesis, leading to enhanced biofilm formation.
33669956	2	14	theme	common	283:288	arg1	component					298:306	a common monomer component	281:306	a common monomer component of dental composite resins	281:333	This study assessed the biological effects of urethane dimethacrylate (UDMA), a common monomer component of dental composite resins, on the cariogenic properties of Streptococcus mutans.
33669956	2	14	theme	common	283:288	arg1	dimethacrylate					258:271	urethane dimethacrylate	249:271	urethane dimethacrylate (UDMA)	249:278	This study assessed the biological effects of urethane dimethacrylate (UDMA), a common monomer component of dental composite resins, on the cariogenic properties of Streptococcus mutans.
33669956	2	15	from	effects	238:244	arg1	properties					354:363	the cariogenic properties	339:363	the cariogenic properties of Streptococcus mutans	339:387	This study assessed the biological effects of urethane dimethacrylate (UDMA), a common monomer component of dental composite resins, on the cariogenic properties of Streptococcus mutans.
33669956	8	16	theme	oxidative	1552:1560	arg1	tolerance					1562:1570	oxidative tolerance	1552:1570	oxidative tolerance	1552:1570	This study suggests that UDMA may potentially contribute to the development of secondary caries around UDMA-containing dental materials by prompting biofilm formation, enhancing oxidative tolerance, and modulating carbon flow.
33669956	3	17	with	adhesion	486:493	arg1	saliva					454:459	saliva	454:459	saliva	454:459	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	3	18	theme	glycolytic	531:540	arg1	profiles					542:549	glycolytic profiles	531:549	glycolytic profiles	531:549	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	6	19	theme	planktonic	1072:1081	arg1	growth					1083:1088	planktonic growth	1072:1088	planktonic growth	1072:1088	UDMA did not significantly affect the viability or planktonic growth of cells, but diminished the ability of S. mutans to metabolize carbohydrates and thus maintain the level of intracellular polysaccharides, although the tendency for sugar transport increased.
33669956	1	20	theme	materials	192:200	arg1	usage					177:181	the increased usage	163:181	the increased usage of these materials	163:200	Concerns regarding unbound monomers in dental composites have increased with the increased usage of these materials.
33669956	5	21	theme	biofilm	1000:1006	arg1	development					1008:1018	biofilm development	1000:1018	biofilm development	1000:1018	Furthermore, UDMA reduced the acid tolerance of S. mutans, but enhanced its tolerance to oxidative stress, thus favoring the early stage of biofilm development.
33669956	6	22	theme	mutans	1133:1138	arg1	ability					1119:1125	the ability	1115:1125	the ability of S. mutans to metabolize carbohydrates and thus maintain the level of intracellular polysaccharides	1115:1227	UDMA did not significantly affect the viability or planktonic growth of cells, but diminished the ability of S. mutans to metabolize carbohydrates and thus maintain the level of intracellular polysaccharides, although the tendency for sugar transport increased.
33669956	1	23	theme	unbound	105:111	arg1	monomers					113:120	unbound monomers	105:120	unbound monomers	105:120	Concerns regarding unbound monomers in dental composites have increased with the increased usage of these materials.
33669956	0	24	theme	Urethane	0:7	arg1	Dimethacrylate					9:22	Urethane Dimethacrylate	0:22	Urethane Dimethacrylate	0:22	Urethane Dimethacrylate Influences the Cariogenic Properties of Streptococcus Mutans.
33669956	2	25	theme	composite	318:326	arg1	resins					328:333	dental composite resins	311:333	dental composite resins	311:333	This study assessed the biological effects of urethane dimethacrylate (UDMA), a common monomer component of dental composite resins, on the cariogenic properties of Streptococcus mutans.
33669956	8	26	theme	secondary	1453:1461	arg1	caries					1463:1468	secondary caries	1453:1468	secondary caries around UDMA-containing dental materials	1453:1508	This study suggests that UDMA may potentially contribute to the development of secondary caries around UDMA-containing dental materials by prompting biofilm formation, enhancing oxidative tolerance, and modulating carbon flow.
33669956	5	27	theme	early	985:989	arg1	stage					991:995	the early stage	981:995	the early stage of biofilm development	981:1018	Furthermore, UDMA reduced the acid tolerance of S. mutans, but enhanced its tolerance to oxidative stress, thus favoring the early stage of biofilm development.
33669956	2	28	theme	dental	311:316	arg1	resins					328:333	dental composite resins	311:333	dental composite resins	311:333	This study assessed the biological effects of urethane dimethacrylate (UDMA), a common monomer component of dental composite resins, on the cariogenic properties of Streptococcus mutans.
33669956	3	29	theme	surface	462:468	arg1	hydrophobicity					470:483	surface hydrophobicity	462:483	surface hydrophobicity	462:483	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	3	30	theme	acid-stress	571:581	arg1	tolerances					583:592	oxidative- and acid-stress tolerances	556:592	oxidative- and acid-stress tolerances of S. mutans	556:605	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	4	31	theme	underlying	757:766	arg1	surfaces					768:775	the underlying surfaces	753:775	the underlying surfaces	753:775	The results indicated that UDMA promotes the adhesion of S. mutans to the underlying surfaces and extracellular polysaccharide synthesis, leading to enhanced biofilm formation.
33669956	1	32	theme	dental	125:130	arg1	composites					132:141	dental composites	125:141	dental composites	125:141	Concerns regarding unbound monomers in dental composites have increased with the increased usage of these materials.
33669956	3	33	theme	sugar	514:518	arg1	transport					520:528	sugar transport	514:528	sugar transport	514:528	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	5	34	theme	mutans	911:916	arg1	tolerance					895:903	the acid tolerance	886:903	the acid tolerance of S. mutans	886:916	Furthermore, UDMA reduced the acid tolerance of S. mutans, but enhanced its tolerance to oxidative stress, thus favoring the early stage of biofilm development.
33669956	2	35	theme	dimethacrylate	258:271	arg1	effects					238:244	the biological effects	223:244	the biological effects of urethane dimethacrylate (UDMA), a common monomer component of dental composite resins, on the cariogenic properties of Streptococcus mutans	223:387	This study assessed the biological effects of urethane dimethacrylate (UDMA), a common monomer component of dental composite resins, on the cariogenic properties of Streptococcus mutans.
33669956	3	36	with	profiles	542:549	arg1	saliva					454:459	saliva	454:459	saliva	454:459	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	3	37	theme	biofilm	418:424	arg1	formation					426:434	biofilm formation	418:434	biofilm formation	418:434	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	0	38	theme	Cariogenic	39:48	arg1	Properties					50:59	the Cariogenic Properties	35:59	the Cariogenic Properties of Streptococcus Mutans	35:83	Urethane Dimethacrylate Influences the Cariogenic Properties of Streptococcus Mutans.
33669956	4	39	theme	mutans	743:748	arg1	adhesion					728:735	the adhesion	724:735	the adhesion of S. mutans to the underlying surfaces and extracellular polysaccharide synthesis	724:818	The results indicated that UDMA promotes the adhesion of S. mutans to the underlying surfaces and extracellular polysaccharide synthesis, leading to enhanced biofilm formation.
33669956	2	40	theme	urethane	249:256	arg1	UDMA					274:277	UDMA	274:277	UDMA	274:277	This study assessed the biological effects of urethane dimethacrylate (UDMA), a common monomer component of dental composite resins, on the cariogenic properties of Streptococcus mutans.
33669956	2	40	theme	urethane	249:256	arg1	component					298:306	a common monomer component	281:306	a common monomer component of dental composite resins	281:333	This study assessed the biological effects of urethane dimethacrylate (UDMA), a common monomer component of dental composite resins, on the cariogenic properties of Streptococcus mutans.
33669956	2	40	theme	urethane	249:256	arg1	dimethacrylate					258:271	urethane dimethacrylate	249:271	urethane dimethacrylate (UDMA)	249:278	This study assessed the biological effects of urethane dimethacrylate (UDMA), a common monomer component of dental composite resins, on the cariogenic properties of Streptococcus mutans.
33669956	3	41	theme	growth	405:410	arg1	rate					412:415	the growth rate	401:415	the growth rate	401:415	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	8	42	theme	carbon	1588:1593	arg1	flow					1595:1598	carbon flow	1588:1598	carbon flow	1588:1598	This study suggests that UDMA may potentially contribute to the development of secondary caries around UDMA-containing dental materials by prompting biofilm formation, enhancing oxidative tolerance, and modulating carbon flow.
33669956	3	43	with	formation	426:434	arg1	saliva					454:459	saliva	454:459	saliva	454:459	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	5	44	theme	development	1008:1018	arg1	stage					991:995	the early stage	981:995	the early stage of biofilm development	981:1018	Furthermore, UDMA reduced the acid tolerance of S. mutans, but enhanced its tolerance to oxidative stress, thus favoring the early stage of biofilm development.
33669956	6	45	theme	cells	1093:1097	arg1	viability					1059:1067	viability	1059:1067	viability	1059:1067	UDMA did not significantly affect the viability or planktonic growth of cells, but diminished the ability of S. mutans to metabolize carbohydrates and thus maintain the level of intracellular polysaccharides, although the tendency for sugar transport increased.
33669956	6	45	theme	cells	1093:1097	arg1	growth					1083:1088	planktonic growth	1072:1088	planktonic growth	1072:1088	UDMA did not significantly affect the viability or planktonic growth of cells, but diminished the ability of S. mutans to metabolize carbohydrates and thus maintain the level of intracellular polysaccharides, although the tendency for sugar transport increased.
33669956	2	46	theme	cariogenic	343:352	arg1	properties					354:363	the cariogenic properties	339:363	the cariogenic properties of Streptococcus mutans	339:387	This study assessed the biological effects of urethane dimethacrylate (UDMA), a common monomer component of dental composite resins, on the cariogenic properties of Streptococcus mutans.
33669956	3	47	with	hydrophobicity	470:483	arg1	saliva					454:459	saliva	454:459	saliva	454:459	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	6	48	theme	polysaccharides	1213:1227	arg1	level					1190:1194	the level	1186:1194	the level of intracellular polysaccharides	1186:1227	UDMA did not significantly affect the viability or planktonic growth of cells, but diminished the ability of S. mutans to metabolize carbohydrates and thus maintain the level of intracellular polysaccharides, although the tendency for sugar transport increased.
33669956	2	49	theme	biological	227:236	arg1	effects					238:244	the biological effects	223:244	the biological effects of urethane dimethacrylate (UDMA), a common monomer component of dental composite resins, on the cariogenic properties of Streptococcus mutans	223:387	This study assessed the biological effects of urethane dimethacrylate (UDMA), a common monomer component of dental composite resins, on the cariogenic properties of Streptococcus mutans.
33669956	4	50	theme	enhanced	832:839	arg1	formation					849:857	enhanced biofilm formation	832:857	enhanced biofilm formation	832:857	The results indicated that UDMA promotes the adhesion of S. mutans to the underlying surfaces and extracellular polysaccharide synthesis, leading to enhanced biofilm formation.
33669956	3	51	theme	glucan	496:501	arg1	synthesis					503:511	glucan synthesis	496:511	glucan synthesis	496:511	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	3	52	with	interaction	437:447	arg1	saliva					454:459	saliva	454:459	saliva	454:459	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	8	53	theme	caries	1463:1468	arg1	development					1438:1448	the development	1434:1448	the development of secondary caries around UDMA-containing dental materials	1434:1508	This study suggests that UDMA may potentially contribute to the development of secondary caries around UDMA-containing dental materials by prompting biofilm formation, enhancing oxidative tolerance, and modulating carbon flow.
33669956	7	54	theme	bacterial	1345:1353	arg1	cells					1355:1359	bacterial cells	1345:1359	bacterial cells	1345:1359	Notably, UDMA did not significantly alter the interactions of bacterial cells with saliva.
33669956	3	55	theme	mutans	600:605	arg1	formation					426:434	biofilm formation	418:434	biofilm formation	418:434	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	3	55	theme	mutans	600:605	arg1	transport					520:528	sugar transport	514:528	sugar transport	514:528	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	3	55	theme	mutans	600:605	arg1	interaction					437:447	interaction	437:447	interaction with saliva	437:459	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	3	55	theme	mutans	600:605	arg1	adhesion					486:493	adhesion	486:493	adhesion	486:493	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	3	55	theme	mutans	600:605	arg1	rate					412:415	the growth rate	401:415	the growth rate	401:415	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	3	55	theme	mutans	600:605	arg1	profiles					542:549	glycolytic profiles	531:549	glycolytic profiles	531:549	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	3	55	theme	mutans	600:605	arg1	tolerances					583:592	oxidative- and acid-stress tolerances	556:592	oxidative- and acid-stress tolerances of S. mutans	556:605	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	3	55	theme	mutans	600:605	arg1	synthesis					503:511	glucan synthesis	496:511	glucan synthesis	496:511	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	3	55	theme	mutans	600:605	arg1	hydrophobicity					470:483	surface hydrophobicity	462:483	surface hydrophobicity	462:483	Changes in the growth rate, biofilm formation, interaction with saliva, surface hydrophobicity, adhesion, glucan synthesis, sugar transport, glycolytic profiles, and oxidative- and acid-stress tolerances of S. mutans were evaluated after growing the cells in the presence and absence of UDMA.
33669956	6	56	dep	viability	1059:1067	arg1	the					1055:1057	the	1055:1057	the	1055:1057	UDMA did not significantly affect the viability or planktonic growth of cells, but diminished the ability of S. mutans to metabolize carbohydrates and thus maintain the level of intracellular polysaccharides, although the tendency for sugar transport increased.
33669956	8	57	theme	dental	1493:1498	arg1	materials					1500:1508	UDMA-containing dental materials	1477:1508	UDMA-containing dental materials	1477:1508	This study suggests that UDMA may potentially contribute to the development of secondary caries around UDMA-containing dental materials by prompting biofilm formation, enhancing oxidative tolerance, and modulating carbon flow.
33669956	5	58	theme	oxidative	949:957	arg1	stress					959:964	oxidative stress	949:964	oxidative stress	949:964	Furthermore, UDMA reduced the acid tolerance of S. mutans, but enhanced its tolerance to oxidative stress, thus favoring the early stage of biofilm development.
33669956	1	59	theme	increased	167:175	arg1	usage					177:181	the increased usage	163:181	the increased usage of these materials	163:200	Concerns regarding unbound monomers in dental composites have increased with the increased usage of these materials.
33669956	7	60	with	interactions	1329:1340	arg1	saliva					1366:1371	saliva	1366:1371	saliva	1366:1371	Notably, UDMA did not significantly alter the interactions of bacterial cells with saliva.
33669956	8	61	theme	UDMA-containing	1477:1491	arg1	materials					1500:1508	UDMA-containing dental materials	1477:1508	UDMA-containing dental materials	1477:1508	This study suggests that UDMA may potentially contribute to the development of secondary caries around UDMA-containing dental materials by prompting biofilm formation, enhancing oxidative tolerance, and modulating carbon flow.
33669956	4	62	theme	polysaccharide	795:808	arg1	synthesis					810:818	extracellular polysaccharide synthesis	781:818	extracellular polysaccharide synthesis	781:818	The results indicated that UDMA promotes the adhesion of S. mutans to the underlying surfaces and extracellular polysaccharide synthesis, leading to enhanced biofilm formation.
33669956	6	63	dep	metabolize	1143:1152	arg1	maintain					1177:1184	maintain	1177:1184	maintain the level of intracellular polysaccharides	1177:1227	UDMA did not significantly affect the viability or planktonic growth of cells, but diminished the ability of S. mutans to metabolize carbohydrates and thus maintain the level of intracellular polysaccharides, although the tendency for sugar transport increased.
33669956	2	64	theme	mutans	382:387	arg1	properties					354:363	the cariogenic properties	339:363	the cariogenic properties of Streptococcus mutans	339:387	This study assessed the biological effects of urethane dimethacrylate (UDMA), a common monomer component of dental composite resins, on the cariogenic properties of Streptococcus mutans.
33669956	0	65	theme	Mutans	78:83	arg1	Properties					50:59	the Cariogenic Properties	35:59	the Cariogenic Properties of Streptococcus Mutans	35:83	Urethane Dimethacrylate Influences the Cariogenic Properties of Streptococcus Mutans.
33669956	4	66	theme	extracellular	781:793	arg1	synthesis					810:818	extracellular polysaccharide synthesis	781:818	extracellular polysaccharide synthesis	781:818	The results indicated that UDMA promotes the adhesion of S. mutans to the underlying surfaces and extracellular polysaccharide synthesis, leading to enhanced biofilm formation.
33669956	2	67	theme	Streptococcus	368:380	arg1	mutans					382:387	Streptococcus mutans	368:387	Streptococcus mutans	368:387	This study assessed the biological effects of urethane dimethacrylate (UDMA), a common monomer component of dental composite resins, on the cariogenic properties of Streptococcus mutans.
33669956	8	68	theme	biofilm	1523:1529	arg1	formation					1531:1539	biofilm formation	1523:1539	biofilm formation	1523:1539	This study suggests that UDMA may potentially contribute to the development of secondary caries around UDMA-containing dental materials by prompting biofilm formation, enhancing oxidative tolerance, and modulating carbon flow.
32981723	0	0	theme	Holstein-Friesian	73:89	arg1	cattle					91:96	Holstein-Friesian cattle	73:96	Holstein-Friesian cattle	73:96	Phenotypic and genetic effects of pregnancy on milk production traits in Holstein-Friesian cattle.
32981723	4	1	theme	production	491:500	arg1	traits					502:507	milk production traits	486:507	milk production traits	486:507	The objective of this study was to estimate phenotypic and genetic effects of pregnancy on milk production traits.
32981723	5	2	theme	test-day	528:535	arg1	records					537:543	test-day records	528:543	test-day records	528:543	For this purpose, test-day records and conception dates of 1,359 first-parity Holstein-Friesian cows were analyzed.
32981723	1	3	theme	lactation	149:157	arg1	initiation					135:144	the initiation	131:144	the initiation of lactation	131:157	Pregnancy is a prerequisite for the initiation of lactation and for maintaining the milk production cycle.
32981723	10	4	theme	protein	1190:1196	arg1	yield					1198:1202	protein yield	1190:1202	protein yield	1190:1202	Milk yield, lactose yield, protein yield, and fat yield of DGAT1 AA cows were more affected by pregnancy than that of DGAT1 KK cows (e.g., the cumulative effects of pregnancy on milk yield were negligible for DGAT1 KK cows and were -443 kg for DGAT1 AA cows).
32981723	10	5	theme	KK	1378:1379	arg1	cows					1381:1384	DGAT1 KK cows	1372:1384	DGAT1 KK cows	1372:1384	Milk yield, lactose yield, protein yield, and fat yield of DGAT1 AA cows were more affected by pregnancy than that of DGAT1 KK cows (e.g., the cumulative effects of pregnancy on milk yield were negligible for DGAT1 KK cows and were -443 kg for DGAT1 AA cows).
32981723	10	6	theme	pregnancy	1328:1336	arg1	effects					1317:1323	the cumulative effects	1302:1323	the cumulative effects of pregnancy on milk yield	1302:1350	Milk yield, lactose yield, protein yield, and fat yield of DGAT1 AA cows were more affected by pregnancy than that of DGAT1 KK cows (e.g., the cumulative effects of pregnancy on milk yield were negligible for DGAT1 KK cows and were -443 kg for DGAT1 AA cows).
32981723	10	6	theme	pregnancy	1328:1336	arg1	negligible					1357:1366	negligible	1357:1366	negligible	1357:1366	Milk yield, lactose yield, protein yield, and fat yield of DGAT1 AA cows were more affected by pregnancy than that of DGAT1 KK cows (e.g., the cumulative effects of pregnancy on milk yield were negligible for DGAT1 KK cows and were -443 kg for DGAT1 AA cows).
32981723	5	7	theme	cows	606:609	arg1	records					537:543	test-day records	528:543	test-day records	528:543	For this purpose, test-day records and conception dates of 1,359 first-parity Holstein-Friesian cows were analyzed.
32981723	5	7	theme	cows	606:609	arg1	dates					560:564	conception dates	549:564	conception dates	549:564	For this purpose, test-day records and conception dates of 1,359 first-parity Holstein-Friesian cows were analyzed.
32981723	4	8	theme	genetic	454:460	arg1	effects					462:468	phenotypic and genetic effects	439:468	phenotypic and genetic effects of pregnancy on milk production traits	439:507	The objective of this study was to estimate phenotypic and genetic effects of pregnancy on milk production traits.
32981723	11	9	theme	AA	1538:1539	arg1	cows					1541:1544	DGAT1 AA cows	1532:1544	DGAT1 AA cows	1532:1544	These results suggest that DGAT1 KK cows may be more suitable for shortening or omitting the dry period than DGAT1 AA cows.
32981723	3	10	theme	pregnancy	356:364	arg1	effects					366:372	pregnancy effects	356:372	pregnancy effects on milk composition	356:392	Furthermore, there might be genetic differences in pregnancy effects on milk composition.
32981723	10	11	theme	DGAT1	1281:1285	arg1	cows					1290:1293	DGAT1 KK cows	1281:1293	DGAT1 KK cows	1281:1293	Milk yield, lactose yield, protein yield, and fat yield of DGAT1 AA cows were more affected by pregnancy than that of DGAT1 KK cows (e.g., the cumulative effects of pregnancy on milk yield were negligible for DGAT1 KK cows and were -443 kg for DGAT1 AA cows).
32981723	9	12	theme	production	1115:1124	arg1	traits					1126:1131	milk production traits	1110:1131	milk production traits	1110:1131	The effects of pregnancy on milk production traits differed for DGAT1 genotypes.
32981723	2	13	theme	genetic	285:291	arg1	evaluation					293:302	the genetic evaluation	281:302	the genetic evaluation	281:302	Pregnancy affects milk production and therefore should be accounted for in the genetic evaluation.
32981723	6	14	theme	Significant	626:636	arg1	effects					638:644	Significant effects	626:644	Significant effects of pregnancy on all milk production traits	626:687	Significant effects of pregnancy on all milk production traits were detected except somatic cell score (e.g., the cumulative effects of pregnancy on milk yield were -247 kg).
32981723	4	15	theme	pregnancy	473:481	arg1	effects					462:468	phenotypic and genetic effects	439:468	phenotypic and genetic effects of pregnancy on milk production traits	439:507	The objective of this study was to estimate phenotypic and genetic effects of pregnancy on milk production traits.
32981723	6	16	theme	pregnancy	762:770	arg1	kg					796:797	-247 kg	791:797	-247 kg	791:797	Significant effects of pregnancy on all milk production traits were detected except somatic cell score (e.g., the cumulative effects of pregnancy on milk yield were -247 kg).
32981723	6	16	theme	pregnancy	762:770	arg1	effects					751:757	the cumulative effects	736:757	the cumulative effects of pregnancy on milk yield	736:784	Significant effects of pregnancy on all milk production traits were detected except somatic cell score (e.g., the cumulative effects of pregnancy on milk yield were -247 kg).
32981723	10	17	theme	KK	1287:1288	arg1	cows					1290:1293	DGAT1 KK cows	1281:1293	DGAT1 KK cows	1281:1293	Milk yield, lactose yield, protein yield, and fat yield of DGAT1 AA cows were more affected by pregnancy than that of DGAT1 KK cows (e.g., the cumulative effects of pregnancy on milk yield were negligible for DGAT1 KK cows and were -443 kg for DGAT1 AA cows).
32981723	10	18	theme	DGAT1	1222:1226	arg1	cows					1231:1234	DGAT1 AA cows	1222:1234	DGAT1 AA cows	1222:1234	Milk yield, lactose yield, protein yield, and fat yield of DGAT1 AA cows were more affected by pregnancy than that of DGAT1 KK cows (e.g., the cumulative effects of pregnancy on milk yield were negligible for DGAT1 KK cows and were -443 kg for DGAT1 AA cows).
32981723	7	19	theme	lactose	838:844	arg1	yield					846:850	lactose yield	838:850	lactose yield	838:850	The pregnancy effects on milk yield, lactose yield, protein yield, fat yield, and fat content were small during early gestation (<150 d) and substantially increased in late gestation.
32981723	8	20	theme	fat	1071:1073	arg1	yield					1075:1079	fat yield	1071:1079	fat yield	1071:1079	The effects of pregnancy on milk protein yield were relatively stronger than those on fat yield.
32981723	7	21	theme	late	969:972	arg1	gestation					974:982	late gestation	969:982	late gestation	969:982	The pregnancy effects on milk yield, lactose yield, protein yield, fat yield, and fat content were small during early gestation (<150 d) and substantially increased in late gestation.
32981723	8	22	theme	pregnancy	1000:1008	arg1	effects					989:995	The effects	985:995	The effects of pregnancy on milk protein yield	985:1030	The effects of pregnancy on milk protein yield were relatively stronger than those on fat yield.
32981723	8	22	theme	pregnancy	1000:1008	arg1	stronger					1048:1055	stronger	1048:1055	stronger	1048:1055	The effects of pregnancy on milk protein yield were relatively stronger than those on fat yield.
32981723	7	23	theme	early	913:917	arg1	gestation					919:927	early gestation	913:927	early gestation (<150 d)	913:936	The pregnancy effects on milk yield, lactose yield, protein yield, fat yield, and fat content were small during early gestation (<150 d) and substantially increased in late gestation.
32981723	7	23	theme	early	913:917	arg1	d					935:935	<150 d	930:935	<150 d	930:935	The pregnancy effects on milk yield, lactose yield, protein yield, fat yield, and fat content were small during early gestation (<150 d) and substantially increased in late gestation.
32981723	10	24	theme	fat	1209:1211	arg1	yield					1213:1217	fat yield	1209:1217	fat yield	1209:1217	Milk yield, lactose yield, protein yield, and fat yield of DGAT1 AA cows were more affected by pregnancy than that of DGAT1 KK cows (e.g., the cumulative effects of pregnancy on milk yield were negligible for DGAT1 KK cows and were -443 kg for DGAT1 AA cows).
32981723	9	25	theme	milk	1110:1113	arg1	traits					1126:1131	milk production traits	1110:1131	milk production traits	1110:1131	The effects of pregnancy on milk production traits differed for DGAT1 genotypes.
32981723	4	26	theme	milk	486:489	arg1	traits					502:507	milk production traits	486:507	milk production traits	486:507	The objective of this study was to estimate phenotypic and genetic effects of pregnancy on milk production traits.
32981723	10	27	from	effects	1317:1323	arg1	yield					1346:1350	milk yield	1341:1350	milk yield	1341:1350	Milk yield, lactose yield, protein yield, and fat yield of DGAT1 AA cows were more affected by pregnancy than that of DGAT1 KK cows (e.g., the cumulative effects of pregnancy on milk yield were negligible for DGAT1 KK cows and were -443 kg for DGAT1 AA cows).
32981723	10	28	theme	cumulative	1306:1315	arg1	effects					1317:1323	the cumulative effects	1302:1323	the cumulative effects of pregnancy on milk yield	1302:1350	Milk yield, lactose yield, protein yield, and fat yield of DGAT1 AA cows were more affected by pregnancy than that of DGAT1 KK cows (e.g., the cumulative effects of pregnancy on milk yield were negligible for DGAT1 KK cows and were -443 kg for DGAT1 AA cows).
32981723	10	28	theme	cumulative	1306:1315	arg1	negligible					1357:1366	negligible	1357:1366	negligible	1357:1366	Milk yield, lactose yield, protein yield, and fat yield of DGAT1 AA cows were more affected by pregnancy than that of DGAT1 KK cows (e.g., the cumulative effects of pregnancy on milk yield were negligible for DGAT1 KK cows and were -443 kg for DGAT1 AA cows).
32981723	6	29	theme	cell	718:721	arg1	score					723:727	somatic cell score	710:727	somatic cell score	710:727	Significant effects of pregnancy on all milk production traits were detected except somatic cell score (e.g., the cumulative effects of pregnancy on milk yield were -247 kg).
32981723	11	30	theme	DGAT1	1532:1536	arg1	cows					1541:1544	DGAT1 AA cows	1532:1544	DGAT1 AA cows	1532:1544	These results suggest that DGAT1 KK cows may be more suitable for shortening or omitting the dry period than DGAT1 AA cows.
32981723	8	31	theme	milk	1013:1016	arg1	yield					1026:1030	milk protein yield	1013:1030	milk protein yield	1013:1030	The effects of pregnancy on milk protein yield were relatively stronger than those on fat yield.
32981723	9	32	theme	DGAT1	1146:1150	arg1	genotypes					1152:1160	DGAT1 genotypes	1146:1160	DGAT1 genotypes	1146:1160	The effects of pregnancy on milk production traits differed for DGAT1 genotypes.
32981723	6	33	theme	somatic	710:716	arg1	score					723:727	somatic cell score	710:727	somatic cell score	710:727	Significant effects of pregnancy on all milk production traits were detected except somatic cell score (e.g., the cumulative effects of pregnancy on milk yield were -247 kg).
32981723	9	34	from	effects	1086:1092	arg1	traits					1126:1131	milk production traits	1110:1131	milk production traits	1110:1131	The effects of pregnancy on milk production traits differed for DGAT1 genotypes.
32981723	0	35	theme	Phenotypic	0:9	arg1	effects					23:29	Phenotypic and genetic effects	0:29	Phenotypic and genetic effects of pregnancy on milk production	0:61	Phenotypic and genetic effects of pregnancy on milk production traits in Holstein-Friesian cattle.
32981723	8	36	from	effects	989:995	arg1	yield					1026:1030	milk protein yield	1013:1030	milk protein yield	1013:1030	The effects of pregnancy on milk protein yield were relatively stronger than those on fat yield.
32981723	10	37	theme	milk	1341:1344	arg1	yield					1346:1350	milk yield	1341:1350	milk yield	1341:1350	Milk yield, lactose yield, protein yield, and fat yield of DGAT1 AA cows were more affected by pregnancy than that of DGAT1 KK cows (e.g., the cumulative effects of pregnancy on milk yield were negligible for DGAT1 KK cows and were -443 kg for DGAT1 AA cows).
32981723	5	38	theme	first-parity	575:586	arg1	cows					606:609	1,359 first-parity Holstein-Friesian cows	569:609	1,359 first-parity Holstein-Friesian cows	569:609	For this purpose, test-day records and conception dates of 1,359 first-parity Holstein-Friesian cows were analyzed.
32981723	10	39	theme	cows	1231:1234	arg1	yield					1213:1217	fat yield	1209:1217	fat yield	1209:1217	Milk yield, lactose yield, protein yield, and fat yield of DGAT1 AA cows were more affected by pregnancy than that of DGAT1 KK cows (e.g., the cumulative effects of pregnancy on milk yield were negligible for DGAT1 KK cows and were -443 kg for DGAT1 AA cows).
32981723	10	39	theme	cows	1231:1234	arg1	yield					1168:1172	Milk yield	1163:1172	Milk yield	1163:1172	Milk yield, lactose yield, protein yield, and fat yield of DGAT1 AA cows were more affected by pregnancy than that of DGAT1 KK cows (e.g., the cumulative effects of pregnancy on milk yield were negligible for DGAT1 KK cows and were -443 kg for DGAT1 AA cows).
32981723	10	39	theme	cows	1231:1234	arg1	yield					1183:1187	lactose yield	1175:1187	lactose yield	1175:1187	Milk yield, lactose yield, protein yield, and fat yield of DGAT1 AA cows were more affected by pregnancy than that of DGAT1 KK cows (e.g., the cumulative effects of pregnancy on milk yield were negligible for DGAT1 KK cows and were -443 kg for DGAT1 AA cows).
32981723	10	39	theme	cows	1231:1234	arg1	yield					1198:1202	protein yield	1190:1202	protein yield	1190:1202	Milk yield, lactose yield, protein yield, and fat yield of DGAT1 AA cows were more affected by pregnancy than that of DGAT1 KK cows (e.g., the cumulative effects of pregnancy on milk yield were negligible for DGAT1 KK cows and were -443 kg for DGAT1 AA cows).
32981723	1	40	theme	milk	183:186	arg1	cycle					199:203	the milk production cycle	179:203	the milk production cycle	179:203	Pregnancy is a prerequisite for the initiation of lactation and for maintaining the milk production cycle.
32981723	7	41	theme	fat	883:885	arg1	content					887:893	fat content	883:893	fat content	883:893	The pregnancy effects on milk yield, lactose yield, protein yield, fat yield, and fat content were small during early gestation (<150 d) and substantially increased in late gestation.
32981723	4	42	from	effects	462:468	arg1	traits					502:507	milk production traits	486:507	milk production traits	486:507	The objective of this study was to estimate phenotypic and genetic effects of pregnancy on milk production traits.
32981723	5	43	theme	Holstein-Friesian	588:604	arg1	cows					606:609	1,359 first-parity Holstein-Friesian cows	569:609	1,359 first-parity Holstein-Friesian cows	569:609	For this purpose, test-day records and conception dates of 1,359 first-parity Holstein-Friesian cows were analyzed.
32981723	7	44	theme	milk	826:829	arg1	yield					831:835	milk yield	826:835	milk yield	826:835	The pregnancy effects on milk yield, lactose yield, protein yield, fat yield, and fat content were small during early gestation (<150 d) and substantially increased in late gestation.
32981723	1	45	theme	production	188:197	arg1	cycle					199:203	the milk production cycle	179:203	the milk production cycle	179:203	Pregnancy is a prerequisite for the initiation of lactation and for maintaining the milk production cycle.
32981723	3	46	theme	milk	377:380	arg1	composition					382:392	milk composition	377:392	milk composition	377:392	Furthermore, there might be genetic differences in pregnancy effects on milk composition.
32981723	8	47	theme	protein	1018:1024	arg1	yield					1026:1030	milk protein yield	1013:1030	milk protein yield	1013:1030	The effects of pregnancy on milk protein yield were relatively stronger than those on fat yield.
32981723	3	48	from	differences	341:351	arg1	composition					382:392	milk composition	377:392	milk composition	377:392	Furthermore, there might be genetic differences in pregnancy effects on milk composition.
32981723	3	48	from	differences	341:351	arg1	effects					366:372	pregnancy effects	356:372	pregnancy effects on milk composition	356:392	Furthermore, there might be genetic differences in pregnancy effects on milk composition.
32981723	10	49	theme	AA	1413:1414	arg1	cows					1416:1419	DGAT1 AA cows	1407:1419	DGAT1 AA cows	1407:1419	Milk yield, lactose yield, protein yield, and fat yield of DGAT1 AA cows were more affected by pregnancy than that of DGAT1 KK cows (e.g., the cumulative effects of pregnancy on milk yield were negligible for DGAT1 KK cows and were -443 kg for DGAT1 AA cows).
32981723	7	50	theme	pregnancy	805:813	arg1	effects					815:821	The pregnancy effects	801:821	The pregnancy effects on milk yield, lactose yield, protein yield, fat yield, and fat content	801:893	The pregnancy effects on milk yield, lactose yield, protein yield, fat yield, and fat content were small during early gestation (<150 d) and substantially increased in late gestation.
32981723	0	51	theme	pregnancy	34:42	arg1	effects					23:29	Phenotypic and genetic effects	0:29	Phenotypic and genetic effects of pregnancy on milk production	0:61	Phenotypic and genetic effects of pregnancy on milk production traits in Holstein-Friesian cattle.
32981723	10	52	theme	DGAT1	1372:1376	arg1	cows					1381:1384	DGAT1 KK cows	1372:1384	DGAT1 KK cows	1372:1384	Milk yield, lactose yield, protein yield, and fat yield of DGAT1 AA cows were more affected by pregnancy than that of DGAT1 KK cows (e.g., the cumulative effects of pregnancy on milk yield were negligible for DGAT1 KK cows and were -443 kg for DGAT1 AA cows).
32981723	4	53	theme	phenotypic	439:448	arg1	effects					462:468	phenotypic and genetic effects	439:468	phenotypic and genetic effects of pregnancy on milk production traits	439:507	The objective of this study was to estimate phenotypic and genetic effects of pregnancy on milk production traits.
32981723	10	54	theme	lactose	1175:1181	arg1	yield					1183:1187	lactose yield	1175:1187	lactose yield	1175:1187	Milk yield, lactose yield, protein yield, and fat yield of DGAT1 AA cows were more affected by pregnancy than that of DGAT1 KK cows (e.g., the cumulative effects of pregnancy on milk yield were negligible for DGAT1 KK cows and were -443 kg for DGAT1 AA cows).
32981723	7	55	from	effects	815:821	arg1	yield					861:865	protein yield	853:865	protein yield	853:865	The pregnancy effects on milk yield, lactose yield, protein yield, fat yield, and fat content were small during early gestation (<150 d) and substantially increased in late gestation.
32981723	7	55	from	effects	815:821	arg1	yield					872:876	fat yield	868:876	fat yield	868:876	The pregnancy effects on milk yield, lactose yield, protein yield, fat yield, and fat content were small during early gestation (<150 d) and substantially increased in late gestation.
32981723	7	55	from	effects	815:821	arg1	content					887:893	fat content	883:893	fat content	883:893	The pregnancy effects on milk yield, lactose yield, protein yield, fat yield, and fat content were small during early gestation (<150 d) and substantially increased in late gestation.
32981723	7	55	from	effects	815:821	arg1	yield					831:835	milk yield	826:835	milk yield	826:835	The pregnancy effects on milk yield, lactose yield, protein yield, fat yield, and fat content were small during early gestation (<150 d) and substantially increased in late gestation.
32981723	7	55	from	effects	815:821	arg1	yield					846:850	lactose yield	838:850	lactose yield	838:850	The pregnancy effects on milk yield, lactose yield, protein yield, fat yield, and fat content were small during early gestation (<150 d) and substantially increased in late gestation.
32981723	11	56	theme	DGAT1	1450:1454	arg1	cows					1459:1462	DGAT1 KK cows	1450:1462	DGAT1 KK cows	1450:1462	These results suggest that DGAT1 KK cows may be more suitable for shortening or omitting the dry period than DGAT1 AA cows.
32981723	11	57	theme	KK	1456:1457	arg1	cows					1459:1462	DGAT1 KK cows	1450:1462	DGAT1 KK cows	1450:1462	These results suggest that DGAT1 KK cows may be more suitable for shortening or omitting the dry period than DGAT1 AA cows.
32981723	0	58	theme	milk	47:50	arg1	production					52:61	milk production	47:61	milk production	47:61	Phenotypic and genetic effects of pregnancy on milk production traits in Holstein-Friesian cattle.
32981723	3	59	from	effects	366:372	arg1	composition					382:392	milk composition	377:392	milk composition	377:392	Furthermore, there might be genetic differences in pregnancy effects on milk composition.
32981723	2	60	theme	milk	224:227	arg1	production					229:238	milk production	224:238	milk production	224:238	Pregnancy affects milk production and therefore should be accounted for in the genetic evaluation.
32981723	3	61	from	composition	382:392	arg1	differences					341:351	genetic differences	333:351	genetic differences in pregnancy effects on milk composition	333:392	Furthermore, there might be genetic differences in pregnancy effects on milk composition.
32981723	10	62	theme	DGAT1	1407:1411	arg1	cows					1416:1419	DGAT1 AA cows	1407:1419	DGAT1 AA cows	1407:1419	Milk yield, lactose yield, protein yield, and fat yield of DGAT1 AA cows were more affected by pregnancy than that of DGAT1 KK cows (e.g., the cumulative effects of pregnancy on milk yield were negligible for DGAT1 KK cows and were -443 kg for DGAT1 AA cows).
32981723	5	63	theme	conception	549:558	arg1	dates					560:564	conception dates	549:564	conception dates	549:564	For this purpose, test-day records and conception dates of 1,359 first-parity Holstein-Friesian cows were analyzed.
32981723	6	64	from	effects	751:757	arg1	yield					780:784	milk yield	775:784	milk yield	775:784	Significant effects of pregnancy on all milk production traits were detected except somatic cell score (e.g., the cumulative effects of pregnancy on milk yield were -247 kg).
32981723	6	65	theme	production	671:680	arg1	traits					682:687	all milk production traits	662:687	all milk production traits	662:687	Significant effects of pregnancy on all milk production traits were detected except somatic cell score (e.g., the cumulative effects of pregnancy on milk yield were -247 kg).
32981723	6	66	theme	milk	775:778	arg1	yield					780:784	milk yield	775:784	milk yield	775:784	Significant effects of pregnancy on all milk production traits were detected except somatic cell score (e.g., the cumulative effects of pregnancy on milk yield were -247 kg).
32981723	4	67	theme	study	417:421	arg1	objective					399:407	The objective	395:407	The objective of this study	395:421	The objective of this study was to estimate phenotypic and genetic effects of pregnancy on milk production traits.
32981723	6	68	from	effects	638:644	arg1	traits					682:687	all milk production traits	662:687	all milk production traits	662:687	Significant effects of pregnancy on all milk production traits were detected except somatic cell score (e.g., the cumulative effects of pregnancy on milk yield were -247 kg).
32981723	6	69	theme	milk	666:669	arg1	traits					682:687	all milk production traits	662:687	all milk production traits	662:687	Significant effects of pregnancy on all milk production traits were detected except somatic cell score (e.g., the cumulative effects of pregnancy on milk yield were -247 kg).
32981723	11	70	theme	dry	1516:1518	arg1	period					1520:1525	the dry period	1512:1525	the dry period than DGAT1 AA cows	1512:1544	These results suggest that DGAT1 KK cows may be more suitable for shortening or omitting the dry period than DGAT1 AA cows.
32981723	6	71	theme	cumulative	740:749	arg1	kg					796:797	-247 kg	791:797	-247 kg	791:797	Significant effects of pregnancy on all milk production traits were detected except somatic cell score (e.g., the cumulative effects of pregnancy on milk yield were -247 kg).
32981723	6	71	theme	cumulative	740:749	arg1	effects					751:757	the cumulative effects	736:757	the cumulative effects of pregnancy on milk yield	736:784	Significant effects of pregnancy on all milk production traits were detected except somatic cell score (e.g., the cumulative effects of pregnancy on milk yield were -247 kg).
32981723	10	72	theme	AA	1228:1229	arg1	cows					1231:1234	DGAT1 AA cows	1222:1234	DGAT1 AA cows	1222:1234	Milk yield, lactose yield, protein yield, and fat yield of DGAT1 AA cows were more affected by pregnancy than that of DGAT1 KK cows (e.g., the cumulative effects of pregnancy on milk yield were negligible for DGAT1 KK cows and were -443 kg for DGAT1 AA cows).
32981723	7	73	theme	fat	868:870	arg1	yield					872:876	fat yield	868:876	fat yield	868:876	The pregnancy effects on milk yield, lactose yield, protein yield, fat yield, and fat content were small during early gestation (<150 d) and substantially increased in late gestation.
32981723	7	74	theme	protein	853:859	arg1	yield					861:865	protein yield	853:865	protein yield	853:865	The pregnancy effects on milk yield, lactose yield, protein yield, fat yield, and fat content were small during early gestation (<150 d) and substantially increased in late gestation.
32981723	10	75	theme	Milk	1163:1166	arg1	yield					1168:1172	Milk yield	1163:1172	Milk yield	1163:1172	Milk yield, lactose yield, protein yield, and fat yield of DGAT1 AA cows were more affected by pregnancy than that of DGAT1 KK cows (e.g., the cumulative effects of pregnancy on milk yield were negligible for DGAT1 KK cows and were -443 kg for DGAT1 AA cows).
32981723	3	76	theme	genetic	333:339	arg1	differences					341:351	genetic differences	333:351	genetic differences in pregnancy effects on milk composition	333:392	Furthermore, there might be genetic differences in pregnancy effects on milk composition.
32981723	0	77	from	effects	23:29	arg1	production					52:61	milk production	47:61	milk production	47:61	Phenotypic and genetic effects of pregnancy on milk production traits in Holstein-Friesian cattle.
32981723	0	78	theme	genetic	15:21	arg1	effects					23:29	Phenotypic and genetic effects	0:29	Phenotypic and genetic effects of pregnancy on milk production	0:61	Phenotypic and genetic effects of pregnancy on milk production traits in Holstein-Friesian cattle.
32981723	9	79	theme	pregnancy	1097:1105	arg1	effects					1086:1092	The effects	1082:1092	The effects of pregnancy on milk production traits	1082:1131	The effects of pregnancy on milk production traits differed for DGAT1 genotypes.
32981723	6	80	theme	pregnancy	649:657	arg1	effects					638:644	Significant effects	626:644	Significant effects of pregnancy on all milk production traits	626:687	Significant effects of pregnancy on all milk production traits were detected except somatic cell score (e.g., the cumulative effects of pregnancy on milk yield were -247 kg).
33088190	3	0	theme	young	473:477	arg1	shoots					479:484	young shoots	473:484	young shoots	473:484	Here, we analyzed the effect of drought and salt stress on cell wall biosynthesis in young shoots and xylem tissues of Populus trichocarpa using transcriptomic and biochemical methods.
33088190	3	1	from	effect	410:415	arg1	biosynthesis					457:468	cell wall biosynthesis	447:468	cell wall biosynthesis in young shoots and xylem tissues of Populus trichocarpa	447:525	Here, we analyzed the effect of drought and salt stress on cell wall biosynthesis in young shoots and xylem tissues of Populus trichocarpa using transcriptomic and biochemical methods.
33088190	0	2	theme	biosynthesis	56:67	arg1	regulation					32:41	the regulation	28:41	the regulation of cell wall biosynthesis in Populus trichocarpa	28:90	Impact of abiotic stress on the regulation of cell wall biosynthesis in Populus trichocarpa.
33088190	6	3	theme	total	801:805	arg1	unchanged					825:833	unchanged	825:833	unchanged	825:833	In contrast, the total lignin amount was unchanged, while the ratio of S/G lignin was significantly decreased in young shoots.
33088190	6	3	theme	total	801:805	arg1	amount					814:819	the total lignin amount	797:819	the total lignin amount	797:819	In contrast, the total lignin amount was unchanged, while the ratio of S/G lignin was significantly decreased in young shoots.
33088190	4	4	theme	xylem	673:677	arg1	formation					679:687	xylem formation	673:687	xylem formation	673:687	Following exposure to abiotic stress, stem tissues reduced vessel sizes, and young shoots increased xylem formation.
33088190	0	5	from	Impact	0:5	arg1	regulation					32:41	the regulation	28:41	the regulation of cell wall biosynthesis in Populus trichocarpa	28:90	Impact of abiotic stress on the regulation of cell wall biosynthesis in Populus trichocarpa.
33088190	7	6	theme	transcriptome	947:959	arg1	analyses					961:968	transcriptome analyses	947:968	transcriptome analyses	947:968	Consistent with these observations, transcriptome analyses show that the expression of a subset of cell wall-related genes is tightly regulated by drought and salt stresses.
33088190	3	7	theme	Populus	507:513	arg1	trichocarpa					515:525	Populus trichocarpa	507:525	Populus trichocarpa	507:525	Here, we analyzed the effect of drought and salt stress on cell wall biosynthesis in young shoots and xylem tissues of Populus trichocarpa using transcriptomic and biochemical methods.
33088190	8	8	theme	genes	1348:1352	arg1	down-regulation					1323:1337	the down-regulation	1319:1337	the down-regulation of these genes	1319:1352	In particular, the expression of a part of genes encoding key enzymes for S-lignin biosynthesis, caffeic acid O-methyltransferase and ferulate 5-hydroxylase, was decreased, suggesting the lower S/G ratio could be partly attributed to the down-regulation of these genes.
33088190	9	9	theme	transcriptional	1387:1401	arg1	strategy					1427:1434	a transcriptional abiotic stress response strategy	1385:1434	a transcriptional abiotic stress response strategy	1385:1434	Together, our data identifies a transcriptional abiotic stress response strategy in poplar, which results in adaptive changes to the plant cell wall.
33088190	0	10	theme	Populus	72:78	arg1	trichocarpa					80:90	Populus trichocarpa	72:90	Populus trichocarpa	72:90	Impact of abiotic stress on the regulation of cell wall biosynthesis in Populus trichocarpa.
33088190	3	11	theme	trichocarpa	515:525	arg1	shoots					479:484	young shoots	473:484	young shoots	473:484	Here, we analyzed the effect of drought and salt stress on cell wall biosynthesis in young shoots and xylem tissues of Populus trichocarpa using transcriptomic and biochemical methods.
33088190	3	11	theme	trichocarpa	515:525	arg1	tissues					496:502	xylem tissues	490:502	xylem tissues	490:502	Here, we analyzed the effect of drought and salt stress on cell wall biosynthesis in young shoots and xylem tissues of Populus trichocarpa using transcriptomic and biochemical methods.
33088190	3	12	theme	wall	452:455	arg1	biosynthesis					457:468	cell wall biosynthesis	447:468	cell wall biosynthesis in young shoots and xylem tissues of Populus trichocarpa	447:525	Here, we analyzed the effect of drought and salt stress on cell wall biosynthesis in young shoots and xylem tissues of Populus trichocarpa using transcriptomic and biochemical methods.
33088190	5	13	theme	cell	757:760	arg1	polysaccharides					767:781	cell wall polysaccharides	757:781	cell wall polysaccharides	757:781	Compositional analyses revealed a reduction in the total amount of cell wall polysaccharides.
33088190	0	14	from	regulation	32:41	arg1	trichocarpa					80:90	Populus trichocarpa	72:90	Populus trichocarpa	72:90	Impact of abiotic stress on the regulation of cell wall biosynthesis in Populus trichocarpa.
33088190	7	15	theme	salt	1070:1073	arg1	stresses					1075:1082	salt stresses	1070:1082	salt stresses	1070:1082	Consistent with these observations, transcriptome analyses show that the expression of a subset of cell wall-related genes is tightly regulated by drought and salt stresses.
33088190	9	16	theme	abiotic	1403:1409	arg1	strategy					1427:1434	a transcriptional abiotic stress response strategy	1385:1434	a transcriptional abiotic stress response strategy	1385:1434	Together, our data identifies a transcriptional abiotic stress response strategy in poplar, which results in adaptive changes to the plant cell wall.
33088190	4	17	theme	young	650:654	arg1	shoots					656:661	young shoots	650:661	young shoots	650:661	Following exposure to abiotic stress, stem tissues reduced vessel sizes, and young shoots increased xylem formation.
33088190	9	18	theme	stress	1411:1416	arg1	strategy					1427:1434	a transcriptional abiotic stress response strategy	1385:1434	a transcriptional abiotic stress response strategy	1385:1434	Together, our data identifies a transcriptional abiotic stress response strategy in poplar, which results in adaptive changes to the plant cell wall.
33088190	1	19	theme	increased	244:252	arg1	stresses					262:269	increased abiotic stresses	244:269	increased abiotic stresses	244:269	Growth of biomass for lignocellulosic biofuels and biomaterials may take place on land unsuitable for foods, meaning the biomass plants are exposed to increased abiotic stresses.
33088190	6	20	theme	S/G	855:857	arg1	lignin					859:864	S/G lignin	855:864	S/G lignin	855:864	In contrast, the total lignin amount was unchanged, while the ratio of S/G lignin was significantly decreased in young shoots.
33088190	9	21	theme	adaptive	1464:1471	arg1	changes					1473:1479	adaptive changes	1464:1479	adaptive changes to the plant cell wall	1464:1502	Together, our data identifies a transcriptional abiotic stress response strategy in poplar, which results in adaptive changes to the plant cell wall.
33088190	3	22	theme	drought	420:426	arg1	effect					410:415	the effect	406:415	the effect of drought and salt stress on cell wall biosynthesis in young shoots and xylem tissues of Populus trichocarpa	406:525	Here, we analyzed the effect of drought and salt stress on cell wall biosynthesis in young shoots and xylem tissues of Populus trichocarpa using transcriptomic and biochemical methods.
33088190	9	23	theme	cell	1494:1497	arg1	wall					1499:1502	the plant cell wall	1484:1502	the plant cell wall	1484:1502	Together, our data identifies a transcriptional abiotic stress response strategy in poplar, which results in adaptive changes to the plant cell wall.
33088190	1	24	theme	lignocellulosic	115:129	arg1	biofuels					131:138	lignocellulosic biofuels	115:138	lignocellulosic biofuels	115:138	Growth of biomass for lignocellulosic biofuels and biomaterials may take place on land unsuitable for foods, meaning the biomass plants are exposed to increased abiotic stresses.
33088190	5	25	theme	total	741:745	arg1	amount					747:752	the total amount	737:752	the total amount of cell wall polysaccharides	737:781	Compositional analyses revealed a reduction in the total amount of cell wall polysaccharides.
33088190	5	25	theme	total	741:745	arg1	polysaccharides					767:781	cell wall polysaccharides	757:781	cell wall polysaccharides	757:781	Compositional analyses revealed a reduction in the total amount of cell wall polysaccharides.
33088190	6	26	theme	lignin	859:864	arg1	ratio					846:850	the ratio	842:850	the ratio of S/G lignin	842:864	In contrast, the total lignin amount was unchanged, while the ratio of S/G lignin was significantly decreased in young shoots.
33088190	2	27	theme	downstream	362:371	arg1	bioprocessing					373:385	downstream bioprocessing	362:385	downstream bioprocessing	362:385	Thus, the understanding how this affects biomass composition and quality is important for downstream bioprocessing.
33088190	8	28	theme	key	1143:1145	arg1	enzymes					1147:1153	key enzymes	1143:1153	key enzymes	1143:1153	In particular, the expression of a part of genes encoding key enzymes for S-lignin biosynthesis, caffeic acid O-methyltransferase and ferulate 5-hydroxylase, was decreased, suggesting the lower S/G ratio could be partly attributed to the down-regulation of these genes.
33088190	1	29	theme	abiotic	254:260	arg1	stresses					262:269	increased abiotic stresses	244:269	increased abiotic stresses	244:269	Growth of biomass for lignocellulosic biofuels and biomaterials may take place on land unsuitable for foods, meaning the biomass plants are exposed to increased abiotic stresses.
33088190	3	30	from	biosynthesis	457:468	arg1	shoots					479:484	young shoots	473:484	young shoots	473:484	Here, we analyzed the effect of drought and salt stress on cell wall biosynthesis in young shoots and xylem tissues of Populus trichocarpa using transcriptomic and biochemical methods.
33088190	3	30	from	biosynthesis	457:468	arg1	tissues					496:502	xylem tissues	490:502	xylem tissues	490:502	Here, we analyzed the effect of drought and salt stress on cell wall biosynthesis in young shoots and xylem tissues of Populus trichocarpa using transcriptomic and biochemical methods.
33088190	3	31	theme	biochemical	552:562	arg1	methods					564:570	transcriptomic and biochemical methods	533:570	transcriptomic and biochemical methods	533:570	Here, we analyzed the effect of drought and salt stress on cell wall biosynthesis in young shoots and xylem tissues of Populus trichocarpa using transcriptomic and biochemical methods.
33088190	1	32	theme	land	175:178	arg1	plants					222:227	land unsuitable for foods, meaning the biomass plants	175:227	land unsuitable for foods, meaning the biomass plants	175:227	Growth of biomass for lignocellulosic biofuels and biomaterials may take place on land unsuitable for foods, meaning the biomass plants are exposed to increased abiotic stresses.
33088190	0	33	theme	stress	18:23	arg1	Impact					0:5	Impact	0:5	Impact of abiotic stress on the regulation of cell wall biosynthesis in Populus trichocarpa.	0:91	Impact of abiotic stress on the regulation of cell wall biosynthesis in Populus trichocarpa.
33088190	8	34	theme	genes	1128:1132	arg1	part					1120:1123	a part	1118:1123	a part of genes encoding key enzymes for S-lignin biosynthesis, caffeic acid O-methyltransferase and ferulate 5-hydroxylase,	1118:1241	In particular, the expression of a part of genes encoding key enzymes for S-lignin biosynthesis, caffeic acid O-methyltransferase and ferulate 5-hydroxylase, was decreased, suggesting the lower S/G ratio could be partly attributed to the down-regulation of these genes.
33088190	8	35	theme	lower	1273:1277	arg1	ratio					1283:1287	the lower S/G ratio	1269:1287	the lower S/G ratio	1269:1287	In particular, the expression of a part of genes encoding key enzymes for S-lignin biosynthesis, caffeic acid O-methyltransferase and ferulate 5-hydroxylase, was decreased, suggesting the lower S/G ratio could be partly attributed to the down-regulation of these genes.
33088190	1	36	theme	unsuitable	180:189	arg1	plants					222:227	land unsuitable for foods, meaning the biomass plants	175:227	land unsuitable for foods, meaning the biomass plants	175:227	Growth of biomass for lignocellulosic biofuels and biomaterials may take place on land unsuitable for foods, meaning the biomass plants are exposed to increased abiotic stresses.
33088190	0	37	theme	abiotic	10:16	arg1	stress					18:23	abiotic stress	10:23	abiotic stress	10:23	Impact of abiotic stress on the regulation of cell wall biosynthesis in Populus trichocarpa.
33088190	7	38	with	Consistent	911:920	arg1	observations					933:944	these observations	927:944	these observations	927:944	Consistent with these observations, transcriptome analyses show that the expression of a subset of cell wall-related genes is tightly regulated by drought and salt stresses.
33088190	5	39	theme	Compositional	690:702	arg1	analyses					704:711	Compositional analyses	690:711	Compositional analyses	690:711	Compositional analyses revealed a reduction in the total amount of cell wall polysaccharides.
33088190	3	40	theme	transcriptomic	533:546	arg1	methods					564:570	transcriptomic and biochemical methods	533:570	transcriptomic and biochemical methods	533:570	Here, we analyzed the effect of drought and salt stress on cell wall biosynthesis in young shoots and xylem tissues of Populus trichocarpa using transcriptomic and biochemical methods.
33088190	4	41	theme	stem	611:614	arg1	tissues					616:622	stem tissues	611:622	stem tissues	611:622	Following exposure to abiotic stress, stem tissues reduced vessel sizes, and young shoots increased xylem formation.
33088190	5	42	theme	wall	762:765	arg1	polysaccharides					767:781	cell wall polysaccharides	757:781	cell wall polysaccharides	757:781	Compositional analyses revealed a reduction in the total amount of cell wall polysaccharides.
33088190	2	43	theme	biomass	313:319	arg1	composition					321:331	biomass composition	313:331	biomass composition	313:331	Thus, the understanding how this affects biomass composition and quality is important for downstream bioprocessing.
33088190	8	44	theme	ferulate	1219:1226	arg1	5-hydroxylase					1228:1240	ferulate 5-hydroxylase	1219:1240	ferulate 5-hydroxylase	1219:1240	In particular, the expression of a part of genes encoding key enzymes for S-lignin biosynthesis, caffeic acid O-methyltransferase and ferulate 5-hydroxylase, was decreased, suggesting the lower S/G ratio could be partly attributed to the down-regulation of these genes.
33088190	8	44	theme	ferulate	1219:1226	arg1	genes					1128:1132	genes	1128:1132	genes encoding key enzymes for S-lignin biosynthesis	1128:1179	In particular, the expression of a part of genes encoding key enzymes for S-lignin biosynthesis, caffeic acid O-methyltransferase and ferulate 5-hydroxylase, was decreased, suggesting the lower S/G ratio could be partly attributed to the down-regulation of these genes.
33088190	4	45	theme	vessel	632:637	arg1	sizes					639:643	vessel sizes	632:643	vessel sizes	632:643	Following exposure to abiotic stress, stem tissues reduced vessel sizes, and young shoots increased xylem formation.
33088190	5	46	theme	polysaccharides	767:781	arg1	amount					747:752	the total amount	737:752	the total amount of cell wall polysaccharides	737:781	Compositional analyses revealed a reduction in the total amount of cell wall polysaccharides.
33088190	5	46	theme	polysaccharides	767:781	arg1	polysaccharides					767:781	cell wall polysaccharides	757:781	cell wall polysaccharides	757:781	Compositional analyses revealed a reduction in the total amount of cell wall polysaccharides.
33088190	1	47	dep	meaning	202:208	arg1	biomass					214:220	the biomass	210:220	meaning the biomass	202:220	Growth of biomass for lignocellulosic biofuels and biomaterials may take place on land unsuitable for foods, meaning the biomass plants are exposed to increased abiotic stresses.
33088190	8	48	theme	S-lignin	1159:1166	arg1	biosynthesis					1168:1179	S-lignin biosynthesis	1159:1179	S-lignin biosynthesis	1159:1179	In particular, the expression of a part of genes encoding key enzymes for S-lignin biosynthesis, caffeic acid O-methyltransferase and ferulate 5-hydroxylase, was decreased, suggesting the lower S/G ratio could be partly attributed to the down-regulation of these genes.
33088190	3	49	theme	salt	432:435	arg1	stress					437:442	salt stress	432:442	salt stress	432:442	Here, we analyzed the effect of drought and salt stress on cell wall biosynthesis in young shoots and xylem tissues of Populus trichocarpa using transcriptomic and biochemical methods.
33088190	6	50	from	unchanged	825:833	arg1	contrast					787:794	contrast	787:794	contrast	787:794	In contrast, the total lignin amount was unchanged, while the ratio of S/G lignin was significantly decreased in young shoots.
33088190	9	51	theme	response	1418:1425	arg1	strategy					1427:1434	a transcriptional abiotic stress response strategy	1385:1434	a transcriptional abiotic stress response strategy	1385:1434	Together, our data identifies a transcriptional abiotic stress response strategy in poplar, which results in adaptive changes to the plant cell wall.
33088190	7	52	theme	wall-related	1015:1026	arg1	genes					1028:1032	cell wall-related genes	1010:1032	cell wall-related genes	1010:1032	Consistent with these observations, transcriptome analyses show that the expression of a subset of cell wall-related genes is tightly regulated by drought and salt stresses.
33088190	5	53	from	reduction	724:732	arg1	amount					747:752	the total amount	737:752	the total amount of cell wall polysaccharides	737:781	Compositional analyses revealed a reduction in the total amount of cell wall polysaccharides.
33088190	5	53	from	reduction	724:732	arg1	polysaccharides					767:781	cell wall polysaccharides	757:781	cell wall polysaccharides	757:781	Compositional analyses revealed a reduction in the total amount of cell wall polysaccharides.
33088190	7	54	theme	subset	1000:1005	arg1	expression					984:993	the expression	980:993	the expression of a subset of cell wall-related genes	980:1032	Consistent with these observations, transcriptome analyses show that the expression of a subset of cell wall-related genes is tightly regulated by drought and salt stresses.
33088190	8	55	theme	caffeic	1182:1188	arg1	O-methyltransferase					1195:1213	caffeic acid O-methyltransferase	1182:1213	caffeic acid O-methyltransferase	1182:1213	In particular, the expression of a part of genes encoding key enzymes for S-lignin biosynthesis, caffeic acid O-methyltransferase and ferulate 5-hydroxylase, was decreased, suggesting the lower S/G ratio could be partly attributed to the down-regulation of these genes.
33088190	8	55	theme	caffeic	1182:1188	arg1	genes					1128:1132	genes	1128:1132	genes encoding key enzymes for S-lignin biosynthesis	1128:1179	In particular, the expression of a part of genes encoding key enzymes for S-lignin biosynthesis, caffeic acid O-methyltransferase and ferulate 5-hydroxylase, was decreased, suggesting the lower S/G ratio could be partly attributed to the down-regulation of these genes.
33088190	7	56	theme	genes	1028:1032	arg1	subset					1000:1005	a subset	998:1005	a subset of cell wall-related genes	998:1032	Consistent with these observations, transcriptome analyses show that the expression of a subset of cell wall-related genes is tightly regulated by drought and salt stresses.
33088190	1	57	theme	biomass	103:109	arg1	Growth					93:98	Growth	93:98	Growth of biomass for lignocellulosic biofuels and biomaterials	93:155	Growth of biomass for lignocellulosic biofuels and biomaterials may take place on land unsuitable for foods, meaning the biomass plants are exposed to increased abiotic stresses.
33088190	9	58	theme	plant	1488:1492	arg1	wall					1499:1502	the plant cell wall	1484:1502	the plant cell wall	1484:1502	Together, our data identifies a transcriptional abiotic stress response strategy in poplar, which results in adaptive changes to the plant cell wall.
33088190	3	59	theme	cell	447:450	arg1	biosynthesis					457:468	cell wall biosynthesis	447:468	cell wall biosynthesis in young shoots and xylem tissues of Populus trichocarpa	447:525	Here, we analyzed the effect of drought and salt stress on cell wall biosynthesis in young shoots and xylem tissues of Populus trichocarpa using transcriptomic and biochemical methods.
33088190	4	60	theme	abiotic	595:601	arg1	stress					603:608	abiotic stress	595:608	abiotic stress	595:608	Following exposure to abiotic stress, stem tissues reduced vessel sizes, and young shoots increased xylem formation.
33088190	7	61	theme	cell	1010:1013	arg1	genes					1028:1032	cell wall-related genes	1010:1032	cell wall-related genes	1010:1032	Consistent with these observations, transcriptome analyses show that the expression of a subset of cell wall-related genes is tightly regulated by drought and salt stresses.
33088190	9	62	from	strategy	1427:1434	arg1	poplar					1439:1444	poplar	1439:1444	poplar	1439:1444	Together, our data identifies a transcriptional abiotic stress response strategy in poplar, which results in adaptive changes to the plant cell wall.
33088190	2	63	dep	understanding	282:294	arg1	affects					305:311	affects	305:311	affects biomass composition and quality	305:343	Thus, the understanding how this affects biomass composition and quality is important for downstream bioprocessing.
33088190	8	64	theme	acid	1190:1193	arg1	O-methyltransferase					1195:1213	caffeic acid O-methyltransferase	1182:1213	caffeic acid O-methyltransferase	1182:1213	In particular, the expression of a part of genes encoding key enzymes for S-lignin biosynthesis, caffeic acid O-methyltransferase and ferulate 5-hydroxylase, was decreased, suggesting the lower S/G ratio could be partly attributed to the down-regulation of these genes.
33088190	8	64	theme	acid	1190:1193	arg1	genes					1128:1132	genes	1128:1132	genes encoding key enzymes for S-lignin biosynthesis	1128:1179	In particular, the expression of a part of genes encoding key enzymes for S-lignin biosynthesis, caffeic acid O-methyltransferase and ferulate 5-hydroxylase, was decreased, suggesting the lower S/G ratio could be partly attributed to the down-regulation of these genes.
33088190	8	65	theme	S/G	1279:1281	arg1	ratio					1283:1287	the lower S/G ratio	1269:1287	the lower S/G ratio	1269:1287	In particular, the expression of a part of genes encoding key enzymes for S-lignin biosynthesis, caffeic acid O-methyltransferase and ferulate 5-hydroxylase, was decreased, suggesting the lower S/G ratio could be partly attributed to the down-regulation of these genes.
33088190	0	66	theme	wall	51:54	arg1	biosynthesis					56:67	cell wall biosynthesis	46:67	cell wall biosynthesis	46:67	Impact of abiotic stress on the regulation of cell wall biosynthesis in Populus trichocarpa.
33088190	6	67	theme	lignin	807:812	arg1	unchanged					825:833	unchanged	825:833	unchanged	825:833	In contrast, the total lignin amount was unchanged, while the ratio of S/G lignin was significantly decreased in young shoots.
33088190	6	67	theme	lignin	807:812	arg1	amount					814:819	the total lignin amount	797:819	the total lignin amount	797:819	In contrast, the total lignin amount was unchanged, while the ratio of S/G lignin was significantly decreased in young shoots.
33088190	3	68	theme	stress	437:442	arg1	effect					410:415	the effect	406:415	the effect of drought and salt stress on cell wall biosynthesis in young shoots and xylem tissues of Populus trichocarpa	406:525	Here, we analyzed the effect of drought and salt stress on cell wall biosynthesis in young shoots and xylem tissues of Populus trichocarpa using transcriptomic and biochemical methods.
33088190	6	69	from	contrast	787:794	arg1	unchanged					825:833	unchanged	825:833	unchanged	825:833	In contrast, the total lignin amount was unchanged, while the ratio of S/G lignin was significantly decreased in young shoots.
33088190	6	69	from	contrast	787:794	arg1	amount					814:819	the total lignin amount	797:819	the total lignin amount	797:819	In contrast, the total lignin amount was unchanged, while the ratio of S/G lignin was significantly decreased in young shoots.
33088190	0	70	theme	cell	46:49	arg1	biosynthesis					56:67	cell wall biosynthesis	46:67	cell wall biosynthesis	46:67	Impact of abiotic stress on the regulation of cell wall biosynthesis in Populus trichocarpa.
33088190	6	71	theme	young	897:901	arg1	shoots					903:908	young shoots	897:908	young shoots	897:908	In contrast, the total lignin amount was unchanged, while the ratio of S/G lignin was significantly decreased in young shoots.
33088190	3	72	theme	xylem	490:494	arg1	tissues					496:502	xylem tissues	490:502	xylem tissues	490:502	Here, we analyzed the effect of drought and salt stress on cell wall biosynthesis in young shoots and xylem tissues of Populus trichocarpa using transcriptomic and biochemical methods.
33088190	8	73	theme	part	1120:1123	arg1	expression					1104:1113	the expression	1100:1113	the expression of a part of genes encoding key enzymes for S-lignin biosynthesis, caffeic acid O-methyltransferase and ferulate 5-hydroxylase,	1100:1241	In particular, the expression of a part of genes encoding key enzymes for S-lignin biosynthesis, caffeic acid O-methyltransferase and ferulate 5-hydroxylase, was decreased, suggesting the lower S/G ratio could be partly attributed to the down-regulation of these genes.
32474697	10	0	theme	knee	2426:2429	arg1	compartments					2406:2417	the different compartments	2392:2417	the different compartments of the knee	2392:2429	CONCLUSIONS Structural bone and cartilage parameter changes showed varying developments and correlations among each other in the different compartments of the knee.
32474697	4	1	dep	femoral	1068:1074	arg1	each					1107:1110	each	1107:1110	each	1107:1110	Measurements were performed in dependence of the anatomical site (femoral vs. tibial, medial and lateral each) to identify regional differences during the osteoarthritic process.
32474697	0	2	with	interaction	76:86	arg1	cartilage					103:111	articular cartilage	93:111	articular cartilage	93:111	Varying development of femoral and tibial subchondral bone tissue and their interaction with articular cartilage during progressing osteoarthritis.
32474697	6	3	theme	femoral	1483:1489	arg1	condyle					1491:1497	the medial femoral condyle	1472:1497	the medial femoral condyle	1472:1497	RESULTS Concerning 3D analysis, the medial femoral condyle and tibia showed the most significant increase in bone volume fraction and a decrease in the trabecular number in group B frequently accompanied by subchondral bone resorption pits and enchondral ossification.
32474697	1	4	theme	bone-cartilage	287:300	arg1	talk					308:311	bone-cartilage cross talk	287:311	bone-cartilage cross talk	287:311	INTRODUCTION Differences between tibial and femoral joint surfaces and knee compartments concerning coupled bone and cartilage turnover or bone-cartilage cross talk have not been previously examined, although the mechanical and biological interaction of the mineralized subchondral tissues with articular cartilage is of great importance for advancing osteoarthritis.
32474697	7	5	dep	show	1756:1759	arg1	enlarges					1844:1851	enlarges	1844:1851	show lower bone volume fraction than the corresponding femoral site and this difference enlarges with advancing OA	1756:1869	Under physiological conditions, tibia plateaus show lower bone volume fraction than the corresponding femoral site and this difference enlarges with advancing OA.
32474697	2	6	theme	joint	648:652	arg1	tissue					664:669	human knee joint cartilage tissue	637:669	human knee joint cartilage tissue	637:669	MATERIALS AND METHODS Therefore, with the help of immunohistochemistry and real-time polymerase chain reaction (RT-PCR), human knee joint cartilage tissue was investigated for expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis).
32474697	4	7	dep	site	1062:1065	arg1	femoral					1068:1074	femoral	1068:1074	femoral	1068:1074	Measurements were performed in dependence of the anatomical site (femoral vs. tibial, medial and lateral each) to identify regional differences during the osteoarthritic process.
32474697	4	7	dep	site	1062:1065	arg1	lateral					1099:1105	lateral	1099:1105	lateral	1099:1105	Measurements were performed in dependence of the anatomical site (femoral vs. tibial, medial and lateral each) to identify regional differences during the osteoarthritic process.
32474697	4	7	dep	site	1062:1065	arg1	medial					1088:1093	medial	1088:1093	medial	1088:1093	Measurements were performed in dependence of the anatomical site (femoral vs. tibial, medial and lateral each) to identify regional differences during the osteoarthritic process.
32474697	4	7	dep	site	1062:1065	arg1	tibial					1080:1085	tibial	1080:1085	tibial	1080:1085	Measurements were performed in dependence of the anatomical site (femoral vs. tibial, medial and lateral each) to identify regional differences during the osteoarthritic process.
32474697	1	8	theme	joint	200:204	arg1	surfaces					206:213	tibial and femoral joint surfaces	181:213	tibial and femoral joint surfaces	181:213	INTRODUCTION Differences between tibial and femoral joint surfaces and knee compartments concerning coupled bone and cartilage turnover or bone-cartilage cross talk have not been previously examined, although the mechanical and biological interaction of the mineralized subchondral tissues with articular cartilage is of great importance for advancing osteoarthritis.
32474697	9	9	theme	bone	2203:2206	arg1	analysis					2224:2231	bone microstructural analysis	2203:2231	bone microstructural analysis	2203:2231	Collagen type II expression levels show faster and varying changes than type I during the OA process, leading to a lower positive or negative correlation with bone microstructural analysis, especially on the tibia plateau.
32474697	6	10	theme	bone	1549:1552	arg1	fraction					1561:1568	bone volume fraction	1549:1568	bone volume fraction	1549:1568	RESULTS Concerning 3D analysis, the medial femoral condyle and tibia showed the most significant increase in bone volume fraction and a decrease in the trabecular number in group B frequently accompanied by subchondral bone resorption pits and enchondral ossification.
32474697	10	11	theme	varying	2334:2340	arg1	developments					2342:2353	varying developments	2334:2353	varying developments	2334:2353	CONCLUSIONS Structural bone and cartilage parameter changes showed varying developments and correlations among each other in the different compartments of the knee.
32474697	8	12	theme	contradictory	1887:1899	arg1	behavior					1901:1908	Partially even contradictory behavior	1872:1908	Partially even contradictory behavior	1872:1908	Partially even contradictory behavior was observed such as trabecular separation at the lateral tibial and medial plateau in osteochondral OA samples of the same patients.
32474697	10	13	theme	different	2396:2404	arg1	compartments					2406:2417	the different compartments	2392:2417	the different compartments of the knee	2392:2429	CONCLUSIONS Structural bone and cartilage parameter changes showed varying developments and correlations among each other in the different compartments of the knee.
32474697	7	14	theme	femoral	1811:1817	arg1	site					1819:1822	the corresponding femoral site	1793:1822	the corresponding femoral site	1793:1822	Under physiological conditions, tibia plateaus show lower bone volume fraction than the corresponding femoral site and this difference enlarges with advancing OA.
32474697	1	15	theme	tissues	430:436	arg1	interaction					387:397	the mechanical and biological interaction	357:397	the mechanical and biological interaction of the mineralized subchondral tissues with articular cartilage	357:461	INTRODUCTION Differences between tibial and femoral joint surfaces and knee compartments concerning coupled bone and cartilage turnover or bone-cartilage cross talk have not been previously examined, although the mechanical and biological interaction of the mineralized subchondral tissues with articular cartilage is of great importance for advancing osteoarthritis.
32474697	2	16	theme	chain	612:616	arg1	reaction					618:625	real-time polymerase chain reaction	591:625	real-time polymerase chain reaction (RT-PCR)	591:634	MATERIALS AND METHODS Therefore, with the help of immunohistochemistry and real-time polymerase chain reaction (RT-PCR), human knee joint cartilage tissue was investigated for expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis).
32474697	2	16	theme	chain	612:616	arg1	RT-PCR					628:633	RT-PCR	628:633	RT-PCR	628:633	MATERIALS AND METHODS Therefore, with the help of immunohistochemistry and real-time polymerase chain reaction (RT-PCR), human knee joint cartilage tissue was investigated for expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis).
32474697	2	17	theme	proteoglycan	814:825	arg1	content					827:833	proteoglycan content	814:833	proteoglycan content (colorimetric analysis)	814:857	MATERIALS AND METHODS Therefore, with the help of immunohistochemistry and real-time polymerase chain reaction (RT-PCR), human knee joint cartilage tissue was investigated for expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis).
32474697	2	17	theme	proteoglycan	814:825	arg1	analysis					849:856	colorimetric analysis	836:856	colorimetric analysis	836:856	MATERIALS AND METHODS Therefore, with the help of immunohistochemistry and real-time polymerase chain reaction (RT-PCR), human knee joint cartilage tissue was investigated for expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis).
32474697	5	18	theme	osteochondral	1256:1268	arg1	samples					1270:1276	34 osteochondral samples	1253:1276	34 osteochondral samples with lesions macroscopically classified as ICRS grade 1b (group A)	1253:1343	From an enduring series of 108 patients undergoing implantation of TKA, 34 osteochondral samples with lesions macroscopically classified as ICRS grade 1b (group A) and 34 samples with ICRS grade 3a/3b lesions (group B) were compared with 21 healthy controls.
32474697	1	19	theme	articular	443:451	arg1	cartilage					453:461	articular cartilage	443:461	articular cartilage	443:461	INTRODUCTION Differences between tibial and femoral joint surfaces and knee compartments concerning coupled bone and cartilage turnover or bone-cartilage cross talk have not been previously examined, although the mechanical and biological interaction of the mineralized subchondral tissues with articular cartilage is of great importance for advancing osteoarthritis.
32474697	1	20	theme	coupled	248:254	arg1	turnover					275:282	coupled bone and cartilage turnover	248:282	coupled bone and cartilage turnover	248:282	INTRODUCTION Differences between tibial and femoral joint surfaces and knee compartments concerning coupled bone and cartilage turnover or bone-cartilage cross talk have not been previously examined, although the mechanical and biological interaction of the mineralized subchondral tissues with articular cartilage is of great importance for advancing osteoarthritis.
32474697	2	21	theme	human	637:641	arg1	tissue					664:669	human knee joint cartilage tissue	637:669	human knee joint cartilage tissue	637:669	MATERIALS AND METHODS Therefore, with the help of immunohistochemistry and real-time polymerase chain reaction (RT-PCR), human knee joint cartilage tissue was investigated for expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis).
32474697	11	22	theme	cartilage	2491:2499	arg1	degeneration					2501:2512	cartilage degeneration	2491:2512	cartilage degeneration	2491:2512	As a clinical conclusion, therapies to postpone or prevent cartilage degeneration by influencing the loss of mineralized bone could be site dependent.
32474697	6	23	from	number	1603:1608	arg1	B					1619:1619	group B	1613:1619	group B frequently accompanied by subchondral bone resorption pits and enchondral ossification	1613:1706	RESULTS Concerning 3D analysis, the medial femoral condyle and tibia showed the most significant increase in bone volume fraction and a decrease in the trabecular number in group B frequently accompanied by subchondral bone resorption pits and enchondral ossification.
32474697	3	24	theme	3D	904:905	arg1	tomography					921:930	3D microcomputed tomography	904:930	3D microcomputed tomography of the underlying subchondral bone (high-resolution micro-CT system)	904:999	Furthermore, we correlated the results with 3D microcomputed tomography of the underlying subchondral bone (high-resolution micro-CT system).
32474697	2	25	theme	cartilage	752:760	arg1	composition					762:772	cartilage composition	752:772	cartilage composition	752:772	MATERIALS AND METHODS Therefore, with the help of immunohistochemistry and real-time polymerase chain reaction (RT-PCR), human knee joint cartilage tissue was investigated for expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis).
32474697	8	26	theme	OA	2011:2012	arg1	samples					2014:2020	osteochondral OA samples	1997:2020	osteochondral OA samples of the same patients	1997:2041	Partially even contradictory behavior was observed such as trabecular separation at the lateral tibial and medial plateau in osteochondral OA samples of the same patients.
32474697	1	27	theme	great	469:473	arg1	importance					475:484	great importance	469:484	great importance	469:484	INTRODUCTION Differences between tibial and femoral joint surfaces and knee compartments concerning coupled bone and cartilage turnover or bone-cartilage cross talk have not been previously examined, although the mechanical and biological interaction of the mineralized subchondral tissues with articular cartilage is of great importance for advancing osteoarthritis.
32474697	6	28	theme	trabecular	1592:1601	arg1	number					1603:1608	the trabecular number	1588:1608	the trabecular number in group B frequently accompanied by subchondral bone resorption pits and enchondral ossification	1588:1706	RESULTS Concerning 3D analysis, the medial femoral condyle and tibia showed the most significant increase in bone volume fraction and a decrease in the trabecular number in group B frequently accompanied by subchondral bone resorption pits and enchondral ossification.
32474697	1	29	theme	INTRODUCTION	148:159	arg1	Differences					161:171	INTRODUCTION Differences	148:171	INTRODUCTION Differences between tibial and femoral joint surfaces	148:213	INTRODUCTION Differences between tibial and femoral joint surfaces and knee compartments concerning coupled bone and cartilage turnover or bone-cartilage cross talk have not been previously examined, although the mechanical and biological interaction of the mineralized subchondral tissues with articular cartilage is of great importance for advancing osteoarthritis.
32474697	7	30	theme	volume	1772:1777	arg1	fraction					1779:1786	lower bone volume fraction	1761:1786	lower bone volume fraction than the corresponding femoral site and this difference	1761:1842	Under physiological conditions, tibia plateaus show lower bone volume fraction than the corresponding femoral site and this difference enlarges with advancing OA.
32474697	10	31	theme	cartilage	2299:2307	arg1	changes					2319:2325	Structural bone and cartilage parameter changes	2279:2325	Structural bone and cartilage parameter changes	2279:2325	CONCLUSIONS Structural bone and cartilage parameter changes showed varying developments and correlations among each other in the different compartments of the knee.
32474697	6	32	theme	enchondral	1684:1693	arg1	ossification					1695:1706	enchondral ossification	1684:1706	enchondral ossification	1684:1706	RESULTS Concerning 3D analysis, the medial femoral condyle and tibia showed the most significant increase in bone volume fraction and a decrease in the trabecular number in group B frequently accompanied by subchondral bone resorption pits and enchondral ossification.
32474697	9	33	theme	Collagen	2044:2051	arg1	type					2053:2056	Collagen type II	2044:2059	Collagen type II expression levels	2044:2077	Collagen type II expression levels show faster and varying changes than type I during the OA process, leading to a lower positive or negative correlation with bone microstructural analysis, especially on the tibia plateau.
32474697	0	34	theme	tissue	59:64	arg1	interaction					76:86	their interaction	70:86	their interaction with articular cartilage	70:111	Varying development of femoral and tibial subchondral bone tissue and their interaction with articular cartilage during progressing osteoarthritis.
32474697	0	34	theme	tissue	59:64	arg1	development					8:18	development	8:18	development of femoral and tibial subchondral bone tissue	8:64	Varying development of femoral and tibial subchondral bone tissue and their interaction with articular cartilage during progressing osteoarthritis.
32474697	1	35	theme	mechanical	361:370	arg1	interaction					387:397	the mechanical and biological interaction	357:397	the mechanical and biological interaction of the mineralized subchondral tissues with articular cartilage	357:461	INTRODUCTION Differences between tibial and femoral joint surfaces and knee compartments concerning coupled bone and cartilage turnover or bone-cartilage cross talk have not been previously examined, although the mechanical and biological interaction of the mineralized subchondral tissues with articular cartilage is of great importance for advancing osteoarthritis.
32474697	6	36	from	decrease	1576:1583	arg1	fraction					1561:1568	bone volume fraction	1549:1568	bone volume fraction	1549:1568	RESULTS Concerning 3D analysis, the medial femoral condyle and tibia showed the most significant increase in bone volume fraction and a decrease in the trabecular number in group B frequently accompanied by subchondral bone resorption pits and enchondral ossification.
32474697	6	36	from	decrease	1576:1583	arg1	number					1603:1608	the trabecular number	1588:1608	the trabecular number in group B frequently accompanied by subchondral bone resorption pits and enchondral ossification	1588:1706	RESULTS Concerning 3D analysis, the medial femoral condyle and tibia showed the most significant increase in bone volume fraction and a decrease in the trabecular number in group B frequently accompanied by subchondral bone resorption pits and enchondral ossification.
32474697	7	37	theme	lower	1761:1765	arg1	fraction					1779:1786	lower bone volume fraction	1761:1786	lower bone volume fraction than the corresponding femoral site and this difference	1761:1842	Under physiological conditions, tibia plateaus show lower bone volume fraction than the corresponding femoral site and this difference enlarges with advancing OA.
32474697	4	38	theme	anatomical	1051:1060	arg1	site					1062:1065	the anatomical site	1047:1065	the anatomical site (femoral vs. tibial, medial and lateral each)	1047:1111	Measurements were performed in dependence of the anatomical site (femoral vs. tibial, medial and lateral each) to identify regional differences during the osteoarthritic process.
32474697	11	39	theme	bone	2553:2556	arg1	loss					2533:2536	the loss	2529:2536	the loss of mineralized bone	2529:2556	As a clinical conclusion, therapies to postpone or prevent cartilage degeneration by influencing the loss of mineralized bone could be site dependent.
32474697	5	40	theme	grade	1326:1330	arg1	1b					1332:1333	ICRS grade 1b	1321:1333	ICRS grade 1b (group A)	1321:1343	From an enduring series of 108 patients undergoing implantation of TKA, 34 osteochondral samples with lesions macroscopically classified as ICRS grade 1b (group A) and 34 samples with ICRS grade 3a/3b lesions (group B) were compared with 21 healthy controls.
32474697	5	40	theme	grade	1326:1330	arg1	A					1342:1342	group A	1336:1342	group A	1336:1342	From an enduring series of 108 patients undergoing implantation of TKA, 34 osteochondral samples with lesions macroscopically classified as ICRS grade 1b (group A) and 34 samples with ICRS grade 3a/3b lesions (group B) were compared with 21 healthy controls.
32474697	8	41	theme	same	2029:2032	arg1	patients					2034:2041	the same patients	2025:2041	the same patients	2025:2041	Partially even contradictory behavior was observed such as trabecular separation at the lateral tibial and medial plateau in osteochondral OA samples of the same patients.
32474697	1	42	theme	biological	376:385	arg1	interaction					387:397	the mechanical and biological interaction	357:397	the mechanical and biological interaction of the mineralized subchondral tissues with articular cartilage	357:461	INTRODUCTION Differences between tibial and femoral joint surfaces and knee compartments concerning coupled bone and cartilage turnover or bone-cartilage cross talk have not been previously examined, although the mechanical and biological interaction of the mineralized subchondral tissues with articular cartilage is of great importance for advancing osteoarthritis.
32474697	2	43	dep	MATERIALS	516:524	arg1	investigated					675:686	investigated	675:686	was investigated for expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis)	671:857	MATERIALS AND METHODS Therefore, with the help of immunohistochemistry and real-time polymerase chain reaction (RT-PCR), human knee joint cartilage tissue was investigated for expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis).
32474697	2	44	theme	real-time	591:599	arg1	reaction					618:625	real-time polymerase chain reaction	591:625	real-time polymerase chain reaction (RT-PCR)	591:634	MATERIALS AND METHODS Therefore, with the help of immunohistochemistry and real-time polymerase chain reaction (RT-PCR), human knee joint cartilage tissue was investigated for expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis).
32474697	2	44	theme	real-time	591:599	arg1	RT-PCR					628:633	RT-PCR	628:633	RT-PCR	628:633	MATERIALS AND METHODS Therefore, with the help of immunohistochemistry and real-time polymerase chain reaction (RT-PCR), human knee joint cartilage tissue was investigated for expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis).
32474697	2	45	theme	molecules	710:718	arg1	expression					692:701	expression	692:701	expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis)	692:857	MATERIALS AND METHODS Therefore, with the help of immunohistochemistry and real-time polymerase chain reaction (RT-PCR), human knee joint cartilage tissue was investigated for expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis).
32474697	3	46	dep	micro-CT	984:991	arg1	system					993:998	system	993:998	high-resolution micro-CT system	968:998	Furthermore, we correlated the results with 3D microcomputed tomography of the underlying subchondral bone (high-resolution micro-CT system).
32474697	6	47	theme	resorption	1664:1673	arg1	pits					1675:1678	subchondral bone resorption pits	1647:1678	subchondral bone resorption pits	1647:1678	RESULTS Concerning 3D analysis, the medial femoral condyle and tibia showed the most significant increase in bone volume fraction and a decrease in the trabecular number in group B frequently accompanied by subchondral bone resorption pits and enchondral ossification.
32474697	9	48	theme	faster	2084:2089	arg1	changes					2103:2109	faster and varying changes	2084:2109	faster and varying changes than type I	2084:2121	Collagen type II expression levels show faster and varying changes than type I during the OA process, leading to a lower positive or negative correlation with bone microstructural analysis, especially on the tibia plateau.
32474697	5	49	theme	3a/3b	1376:1380	arg1	lesions					1382:1388	ICRS grade 3a/3b lesions	1365:1388	ICRS grade 3a/3b lesions (group B)	1365:1398	From an enduring series of 108 patients undergoing implantation of TKA, 34 osteochondral samples with lesions macroscopically classified as ICRS grade 1b (group A) and 34 samples with ICRS grade 3a/3b lesions (group B) were compared with 21 healthy controls.
32474697	5	49	theme	3a/3b	1376:1380	arg1	B					1397:1397	group B	1391:1397	group B	1391:1397	From an enduring series of 108 patients undergoing implantation of TKA, 34 osteochondral samples with lesions macroscopically classified as ICRS grade 1b (group A) and 34 samples with ICRS grade 3a/3b lesions (group B) were compared with 21 healthy controls.
32474697	1	50	theme	mineralized	406:416	arg1	tissues					430:436	the mineralized subchondral tissues	402:436	the mineralized subchondral tissues	402:436	INTRODUCTION Differences between tibial and femoral joint surfaces and knee compartments concerning coupled bone and cartilage turnover or bone-cartilage cross talk have not been previously examined, although the mechanical and biological interaction of the mineralized subchondral tissues with articular cartilage is of great importance for advancing osteoarthritis.
32474697	3	51	dep	tomography	921:930	arg1	micro-CT					984:991	high-resolution micro-CT	968:991	high-resolution micro-CT system	968:998	Furthermore, we correlated the results with 3D microcomputed tomography of the underlying subchondral bone (high-resolution micro-CT system).
32474697	3	52	theme	subchondral	950:960	arg1	bone					962:965	the underlying subchondral bone	935:965	the underlying subchondral bone	935:965	Furthermore, we correlated the results with 3D microcomputed tomography of the underlying subchondral bone (high-resolution micro-CT system).
32474697	2	53	theme	matrix	741:746	arg1	molecules					710:718	key molecules	706:718	key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis)	706:857	MATERIALS AND METHODS Therefore, with the help of immunohistochemistry and real-time polymerase chain reaction (RT-PCR), human knee joint cartilage tissue was investigated for expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis).
32474697	10	54	theme	bone	2290:2293	arg1	changes					2319:2325	Structural bone and cartilage parameter changes	2279:2325	Structural bone and cartilage parameter changes	2279:2325	CONCLUSIONS Structural bone and cartilage parameter changes showed varying developments and correlations among each other in the different compartments of the knee.
32474697	6	55	theme	3D	1459:1460	arg1	analysis					1462:1469	3D analysis	1459:1469	3D analysis	1459:1469	RESULTS Concerning 3D analysis, the medial femoral condyle and tibia showed the most significant increase in bone volume fraction and a decrease in the trabecular number in group B frequently accompanied by subchondral bone resorption pits and enchondral ossification.
32474697	5	56	theme	TKA	1248:1250	arg1	implantation					1232:1243	implantation	1232:1243	implantation of TKA	1232:1250	From an enduring series of 108 patients undergoing implantation of TKA, 34 osteochondral samples with lesions macroscopically classified as ICRS grade 1b (group A) and 34 samples with ICRS grade 3a/3b lesions (group B) were compared with 21 healthy controls.
32474697	5	57	theme	ICRS	1365:1368	arg1	lesions					1382:1388	ICRS grade 3a/3b lesions	1365:1388	ICRS grade 3a/3b lesions (group B)	1365:1398	From an enduring series of 108 patients undergoing implantation of TKA, 34 osteochondral samples with lesions macroscopically classified as ICRS grade 1b (group A) and 34 samples with ICRS grade 3a/3b lesions (group B) were compared with 21 healthy controls.
32474697	5	57	theme	ICRS	1365:1368	arg1	B					1397:1397	group B	1391:1397	group B	1391:1397	From an enduring series of 108 patients undergoing implantation of TKA, 34 osteochondral samples with lesions macroscopically classified as ICRS grade 1b (group A) and 34 samples with ICRS grade 3a/3b lesions (group B) were compared with 21 healthy controls.
32474697	0	58	theme	subchondral	42:52	arg1	tissue					59:64	femoral and tibial subchondral bone tissue	23:64	femoral and tibial subchondral bone tissue	23:64	Varying development of femoral and tibial subchondral bone tissue and their interaction with articular cartilage during progressing osteoarthritis.
32474697	5	59	theme	patients	1212:1219	arg1	series					1198:1203	an enduring series	1186:1203	an enduring series of 108 patients undergoing implantation of TKA	1186:1250	From an enduring series of 108 patients undergoing implantation of TKA, 34 osteochondral samples with lesions macroscopically classified as ICRS grade 1b (group A) and 34 samples with ICRS grade 3a/3b lesions (group B) were compared with 21 healthy controls.
32474697	4	60	theme	regional	1125:1132	arg1	differences					1134:1144	regional differences	1125:1144	regional differences	1125:1144	Measurements were performed in dependence of the anatomical site (femoral vs. tibial, medial and lateral each) to identify regional differences during the osteoarthritic process.
32474697	8	61	from	separation	1942:1951	arg1	samples					2014:2020	osteochondral OA samples	1997:2020	osteochondral OA samples of the same patients	1997:2041	Partially even contradictory behavior was observed such as trabecular separation at the lateral tibial and medial plateau in osteochondral OA samples of the same patients.
32474697	5	62	theme	healthy	1422:1428	arg1	controls					1430:1437	21 healthy controls	1419:1437	21 healthy controls	1419:1437	From an enduring series of 108 patients undergoing implantation of TKA, 34 osteochondral samples with lesions macroscopically classified as ICRS grade 1b (group A) and 34 samples with ICRS grade 3a/3b lesions (group B) were compared with 21 healthy controls.
32474697	7	63	theme	bone	1767:1770	arg1	fraction					1779:1786	lower bone volume fraction	1761:1786	lower bone volume fraction than the corresponding femoral site and this difference	1761:1842	Under physiological conditions, tibia plateaus show lower bone volume fraction than the corresponding femoral site and this difference enlarges with advancing OA.
32474697	1	64	theme	bone	256:259	arg1	turnover					275:282	coupled bone and cartilage turnover	248:282	coupled bone and cartilage turnover	248:282	INTRODUCTION Differences between tibial and femoral joint surfaces and knee compartments concerning coupled bone and cartilage turnover or bone-cartilage cross talk have not been previously examined, although the mechanical and biological interaction of the mineralized subchondral tissues with articular cartilage is of great importance for advancing osteoarthritis.
32474697	2	65	dep	the	554:556	arg1	help					558:561	help	558:561	help	558:561	MATERIALS AND METHODS Therefore, with the help of immunohistochemistry and real-time polymerase chain reaction (RT-PCR), human knee joint cartilage tissue was investigated for expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis).
32474697	1	66	theme	cartilage	265:273	arg1	turnover					275:282	coupled bone and cartilage turnover	248:282	coupled bone and cartilage turnover	248:282	INTRODUCTION Differences between tibial and femoral joint surfaces and knee compartments concerning coupled bone and cartilage turnover or bone-cartilage cross talk have not been previously examined, although the mechanical and biological interaction of the mineralized subchondral tissues with articular cartilage is of great importance for advancing osteoarthritis.
32474697	8	67	from	tibial	1968:1973	arg1	separation					1942:1951	trabecular separation	1931:1951	trabecular separation at the lateral tibial and medial plateau in osteochondral OA samples of the same patients	1931:2041	Partially even contradictory behavior was observed such as trabecular separation at the lateral tibial and medial plateau in osteochondral OA samples of the same patients.
32474697	5	68	theme	34	1253:1254	arg1	samples					1270:1276	34 osteochondral samples	1253:1276	34 osteochondral samples with lesions macroscopically classified as ICRS grade 1b (group A)	1253:1343	From an enduring series of 108 patients undergoing implantation of TKA, 34 osteochondral samples with lesions macroscopically classified as ICRS grade 1b (group A) and 34 samples with ICRS grade 3a/3b lesions (group B) were compared with 21 healthy controls.
32474697	2	69	theme	cartilage	654:662	arg1	tissue					664:669	human knee joint cartilage tissue	637:669	human knee joint cartilage tissue	637:669	MATERIALS AND METHODS Therefore, with the help of immunohistochemistry and real-time polymerase chain reaction (RT-PCR), human knee joint cartilage tissue was investigated for expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis).
32474697	9	70	theme	positive	2165:2172	arg1	correlation					2186:2196	a lower positive or negative correlation	2157:2196	a lower positive or negative correlation with bone microstructural analysis, especially on the tibia plateau	2157:2264	Collagen type II expression levels show faster and varying changes than type I during the OA process, leading to a lower positive or negative correlation with bone microstructural analysis, especially on the tibia plateau.
32474697	8	71	theme	trabecular	1931:1940	arg1	separation					1942:1951	trabecular separation	1931:1951	trabecular separation at the lateral tibial and medial plateau in osteochondral OA samples of the same patients	1931:2041	Partially even contradictory behavior was observed such as trabecular separation at the lateral tibial and medial plateau in osteochondral OA samples of the same patients.
32474697	1	72	theme	advancing	490:498	arg1	osteoarthritis					500:513	advancing osteoarthritis	490:513	advancing osteoarthritis	490:513	INTRODUCTION Differences between tibial and femoral joint surfaces and knee compartments concerning coupled bone and cartilage turnover or bone-cartilage cross talk have not been previously examined, although the mechanical and biological interaction of the mineralized subchondral tissues with articular cartilage is of great importance for advancing osteoarthritis.
32474697	6	73	theme	medial	1476:1481	arg1	condyle					1491:1497	the medial femoral condyle	1472:1497	the medial femoral condyle	1472:1497	RESULTS Concerning 3D analysis, the medial femoral condyle and tibia showed the most significant increase in bone volume fraction and a decrease in the trabecular number in group B frequently accompanied by subchondral bone resorption pits and enchondral ossification.
32474697	1	74	theme	cross	302:306	arg1	talk					308:311	bone-cartilage cross talk	287:311	bone-cartilage cross talk	287:311	INTRODUCTION Differences between tibial and femoral joint surfaces and knee compartments concerning coupled bone and cartilage turnover or bone-cartilage cross talk have not been previously examined, although the mechanical and biological interaction of the mineralized subchondral tissues with articular cartilage is of great importance for advancing osteoarthritis.
32474697	2	75	theme	knee	643:646	arg1	tissue					664:669	human knee joint cartilage tissue	637:669	human knee joint cartilage tissue	637:669	MATERIALS AND METHODS Therefore, with the help of immunohistochemistry and real-time polymerase chain reaction (RT-PCR), human knee joint cartilage tissue was investigated for expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis).
32474697	9	76	theme	negative	2177:2184	arg1	correlation					2186:2196	a lower positive or negative correlation	2157:2196	a lower positive or negative correlation with bone microstructural analysis, especially on the tibia plateau	2157:2264	Collagen type II expression levels show faster and varying changes than type I during the OA process, leading to a lower positive or negative correlation with bone microstructural analysis, especially on the tibia plateau.
32474697	9	77	from	analysis	2224:2231	arg1	plateau					2258:2264	the tibia plateau	2248:2264	the tibia plateau	2248:2264	Collagen type II expression levels show faster and varying changes than type I during the OA process, leading to a lower positive or negative correlation with bone microstructural analysis, especially on the tibia plateau.
32474697	3	78	theme	underlying	939:948	arg1	bone					962:965	the underlying subchondral bone	935:965	the underlying subchondral bone	935:965	Furthermore, we correlated the results with 3D microcomputed tomography of the underlying subchondral bone (high-resolution micro-CT system).
32474697	1	79	theme	femoral	192:198	arg1	surfaces					206:213	tibial and femoral joint surfaces	181:213	tibial and femoral joint surfaces	181:213	INTRODUCTION Differences between tibial and femoral joint surfaces and knee compartments concerning coupled bone and cartilage turnover or bone-cartilage cross talk have not been previously examined, although the mechanical and biological interaction of the mineralized subchondral tissues with articular cartilage is of great importance for advancing osteoarthritis.
32474697	6	80	theme	volume	1554:1559	arg1	fraction					1561:1568	bone volume fraction	1549:1568	bone volume fraction	1549:1568	RESULTS Concerning 3D analysis, the medial femoral condyle and tibia showed the most significant increase in bone volume fraction and a decrease in the trabecular number in group B frequently accompanied by subchondral bone resorption pits and enchondral ossification.
32474697	5	81	theme	enduring	1189:1196	arg1	series					1198:1203	an enduring series	1186:1203	an enduring series of 108 patients undergoing implantation of TKA	1186:1250	From an enduring series of 108 patients undergoing implantation of TKA, 34 osteochondral samples with lesions macroscopically classified as ICRS grade 1b (group A) and 34 samples with ICRS grade 3a/3b lesions (group B) were compared with 21 healthy controls.
32474697	7	82	theme	corresponding	1797:1809	arg1	site					1819:1822	the corresponding femoral site	1793:1822	the corresponding femoral site	1793:1822	Under physiological conditions, tibia plateaus show lower bone volume fraction than the corresponding femoral site and this difference enlarges with advancing OA.
32474697	2	83	theme	colorimetric	836:847	arg1	content					827:833	proteoglycan content	814:833	proteoglycan content (colorimetric analysis)	814:857	MATERIALS AND METHODS Therefore, with the help of immunohistochemistry and real-time polymerase chain reaction (RT-PCR), human knee joint cartilage tissue was investigated for expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis).
32474697	2	83	theme	colorimetric	836:847	arg1	analysis					849:856	colorimetric analysis	836:856	colorimetric analysis	836:856	MATERIALS AND METHODS Therefore, with the help of immunohistochemistry and real-time polymerase chain reaction (RT-PCR), human knee joint cartilage tissue was investigated for expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis).
32474697	9	84	theme	microstructural	2208:2222	arg1	analysis					2224:2231	bone microstructural analysis	2203:2231	bone microstructural analysis	2203:2231	Collagen type II expression levels show faster and varying changes than type I during the OA process, leading to a lower positive or negative correlation with bone microstructural analysis, especially on the tibia plateau.
32474697	10	85	theme	Structural	2279:2288	arg1	changes					2319:2325	Structural bone and cartilage parameter changes	2279:2325	Structural bone and cartilage parameter changes	2279:2325	CONCLUSIONS Structural bone and cartilage parameter changes showed varying developments and correlations among each other in the different compartments of the knee.
32474697	1	86	theme	knee	219:222	arg1	compartments					224:235	knee compartments	219:235	knee compartments concerning coupled bone and cartilage turnover or bone-cartilage cross talk	219:311	INTRODUCTION Differences between tibial and femoral joint surfaces and knee compartments concerning coupled bone and cartilage turnover or bone-cartilage cross talk have not been previously examined, although the mechanical and biological interaction of the mineralized subchondral tissues with articular cartilage is of great importance for advancing osteoarthritis.
32474697	2	87	theme	content	827:833	arg1	molecules					710:718	key molecules	706:718	key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis)	706:857	MATERIALS AND METHODS Therefore, with the help of immunohistochemistry and real-time polymerase chain reaction (RT-PCR), human knee joint cartilage tissue was investigated for expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis).
32474697	4	88	theme	osteoarthritic	1157:1170	arg1	process					1172:1178	the osteoarthritic process	1153:1178	the osteoarthritic process	1153:1178	Measurements were performed in dependence of the anatomical site (femoral vs. tibial, medial and lateral each) to identify regional differences during the osteoarthritic process.
32474697	6	89	theme	significant	1525:1535	arg1	increase					1537:1544	the most significant increase	1516:1544	the most significant increase in bone volume fraction	1516:1568	RESULTS Concerning 3D analysis, the medial femoral condyle and tibia showed the most significant increase in bone volume fraction and a decrease in the trabecular number in group B frequently accompanied by subchondral bone resorption pits and enchondral ossification.
32474697	9	90	with	correlation	2186:2196	arg1	analysis					2224:2231	bone microstructural analysis	2203:2231	bone microstructural analysis	2203:2231	Collagen type II expression levels show faster and varying changes than type I during the OA process, leading to a lower positive or negative correlation with bone microstructural analysis, especially on the tibia plateau.
32474697	2	91	theme	polymerase	601:610	arg1	reaction					618:625	real-time polymerase chain reaction	591:625	real-time polymerase chain reaction (RT-PCR)	591:634	MATERIALS AND METHODS Therefore, with the help of immunohistochemistry and real-time polymerase chain reaction (RT-PCR), human knee joint cartilage tissue was investigated for expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis).
32474697	2	91	theme	polymerase	601:610	arg1	RT-PCR					628:633	RT-PCR	628:633	RT-PCR	628:633	MATERIALS AND METHODS Therefore, with the help of immunohistochemistry and real-time polymerase chain reaction (RT-PCR), human knee joint cartilage tissue was investigated for expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis).
32474697	2	92	theme	collagen	775:782	arg1	aggrecan					799:806	aggrecan	799:806	aggrecan	799:806	MATERIALS AND METHODS Therefore, with the help of immunohistochemistry and real-time polymerase chain reaction (RT-PCR), human knee joint cartilage tissue was investigated for expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis).
32474697	2	92	theme	collagen	775:782	arg1	type					784:787	collagen type I and II	775:796	type	784:787	MATERIALS AND METHODS Therefore, with the help of immunohistochemistry and real-time polymerase chain reaction (RT-PCR), human knee joint cartilage tissue was investigated for expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis).
32474697	8	93	theme	osteochondral	1997:2009	arg1	samples					2014:2020	osteochondral OA samples	1997:2020	osteochondral OA samples of the same patients	1997:2041	Partially even contradictory behavior was observed such as trabecular separation at the lateral tibial and medial plateau in osteochondral OA samples of the same patients.
32474697	5	94	with	samples	1352:1358	arg1	lesions					1382:1388	ICRS grade 3a/3b lesions	1365:1388	ICRS grade 3a/3b lesions (group B)	1365:1398	From an enduring series of 108 patients undergoing implantation of TKA, 34 osteochondral samples with lesions macroscopically classified as ICRS grade 1b (group A) and 34 samples with ICRS grade 3a/3b lesions (group B) were compared with 21 healthy controls.
32474697	5	94	with	samples	1352:1358	arg1	B					1397:1397	group B	1391:1397	group B	1391:1397	From an enduring series of 108 patients undergoing implantation of TKA, 34 osteochondral samples with lesions macroscopically classified as ICRS grade 1b (group A) and 34 samples with ICRS grade 3a/3b lesions (group B) were compared with 21 healthy controls.
32474697	5	94	with	samples	1352:1358	arg1	lesions					1283:1289	lesions	1283:1289	lesions macroscopically classified as ICRS grade 1b (group A)	1283:1343	From an enduring series of 108 patients undergoing implantation of TKA, 34 osteochondral samples with lesions macroscopically classified as ICRS grade 1b (group A) and 34 samples with ICRS grade 3a/3b lesions (group B) were compared with 21 healthy controls.
32474697	6	95	theme	group	1613:1617	arg1	B					1619:1619	group B	1613:1619	group B frequently accompanied by subchondral bone resorption pits and enchondral ossification	1613:1706	RESULTS Concerning 3D analysis, the medial femoral condyle and tibia showed the most significant increase in bone volume fraction and a decrease in the trabecular number in group B frequently accompanied by subchondral bone resorption pits and enchondral ossification.
32474697	2	96	theme	composition	762:772	arg1	molecules					710:718	key molecules	706:718	key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis)	706:857	MATERIALS AND METHODS Therefore, with the help of immunohistochemistry and real-time polymerase chain reaction (RT-PCR), human knee joint cartilage tissue was investigated for expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis).
32474697	5	97	theme	group	1336:1340	arg1	1b					1332:1333	ICRS grade 1b	1321:1333	ICRS grade 1b (group A)	1321:1343	From an enduring series of 108 patients undergoing implantation of TKA, 34 osteochondral samples with lesions macroscopically classified as ICRS grade 1b (group A) and 34 samples with ICRS grade 3a/3b lesions (group B) were compared with 21 healthy controls.
32474697	5	97	theme	group	1336:1340	arg1	A					1342:1342	group A	1336:1342	group A	1336:1342	From an enduring series of 108 patients undergoing implantation of TKA, 34 osteochondral samples with lesions macroscopically classified as ICRS grade 1b (group A) and 34 samples with ICRS grade 3a/3b lesions (group B) were compared with 21 healthy controls.
32474697	9	98	theme	tibia	2252:2256	arg1	plateau					2258:2264	the tibia plateau	2248:2264	the tibia plateau	2248:2264	Collagen type II expression levels show faster and varying changes than type I during the OA process, leading to a lower positive or negative correlation with bone microstructural analysis, especially on the tibia plateau.
32474697	11	99	theme	clinical	2437:2444	arg1	conclusion					2446:2455	a clinical conclusion	2435:2455	a clinical conclusion	2435:2455	As a clinical conclusion, therapies to postpone or prevent cartilage degeneration by influencing the loss of mineralized bone could be site dependent.
32474697	3	100	theme	microcomputed	907:919	arg1	tomography					921:930	3D microcomputed tomography	904:930	3D microcomputed tomography of the underlying subchondral bone (high-resolution micro-CT system)	904:999	Furthermore, we correlated the results with 3D microcomputed tomography of the underlying subchondral bone (high-resolution micro-CT system).
32474697	2	101	dep	matrix	741:746	arg1	aggrecan					799:806	aggrecan	799:806	aggrecan	799:806	MATERIALS AND METHODS Therefore, with the help of immunohistochemistry and real-time polymerase chain reaction (RT-PCR), human knee joint cartilage tissue was investigated for expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis).
32474697	2	101	dep	matrix	741:746	arg1	type					784:787	collagen type I and II	775:796	type	784:787	MATERIALS AND METHODS Therefore, with the help of immunohistochemistry and real-time polymerase chain reaction (RT-PCR), human knee joint cartilage tissue was investigated for expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis).
32474697	2	101	dep	matrix	741:746	arg1	II					795:796	II	795:796	II	795:796	MATERIALS AND METHODS Therefore, with the help of immunohistochemistry and real-time polymerase chain reaction (RT-PCR), human knee joint cartilage tissue was investigated for expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis).
32474697	11	102	theme	mineralized	2541:2551	arg1	bone					2553:2556	mineralized bone	2541:2556	mineralized bone	2541:2556	As a clinical conclusion, therapies to postpone or prevent cartilage degeneration by influencing the loss of mineralized bone could be site dependent.
32474697	9	103	theme	type	2053:2056	arg1	levels					2072:2077	Collagen type II expression levels	2044:2077	Collagen type II expression levels	2044:2077	Collagen type II expression levels show faster and varying changes than type I during the OA process, leading to a lower positive or negative correlation with bone microstructural analysis, especially on the tibia plateau.
32474697	5	104	theme	ICRS	1321:1324	arg1	1b					1332:1333	ICRS grade 1b	1321:1333	ICRS grade 1b (group A)	1321:1343	From an enduring series of 108 patients undergoing implantation of TKA, 34 osteochondral samples with lesions macroscopically classified as ICRS grade 1b (group A) and 34 samples with ICRS grade 3a/3b lesions (group B) were compared with 21 healthy controls.
32474697	5	104	theme	ICRS	1321:1324	arg1	A					1342:1342	group A	1336:1342	group A	1336:1342	From an enduring series of 108 patients undergoing implantation of TKA, 34 osteochondral samples with lesions macroscopically classified as ICRS grade 1b (group A) and 34 samples with ICRS grade 3a/3b lesions (group B) were compared with 21 healthy controls.
32474697	8	105	theme	patients	2034:2041	arg1	samples					2014:2020	osteochondral OA samples	1997:2020	osteochondral OA samples of the same patients	1997:2041	Partially even contradictory behavior was observed such as trabecular separation at the lateral tibial and medial plateau in osteochondral OA samples of the same patients.
32474697	1	106	theme	tibial	181:186	arg1	surfaces					206:213	tibial and femoral joint surfaces	181:213	tibial and femoral joint surfaces	181:213	INTRODUCTION Differences between tibial and femoral joint surfaces and knee compartments concerning coupled bone and cartilage turnover or bone-cartilage cross talk have not been previously examined, although the mechanical and biological interaction of the mineralized subchondral tissues with articular cartilage is of great importance for advancing osteoarthritis.
32474697	1	107	with	interaction	387:397	arg1	cartilage					453:461	articular cartilage	443:461	articular cartilage	443:461	INTRODUCTION Differences between tibial and femoral joint surfaces and knee compartments concerning coupled bone and cartilage turnover or bone-cartilage cross talk have not been previously examined, although the mechanical and biological interaction of the mineralized subchondral tissues with articular cartilage is of great importance for advancing osteoarthritis.
32474697	9	108	theme	expression	2061:2070	arg1	levels					2072:2077	Collagen type II expression levels	2044:2077	Collagen type II expression levels	2044:2077	Collagen type II expression levels show faster and varying changes than type I during the OA process, leading to a lower positive or negative correlation with bone microstructural analysis, especially on the tibia plateau.
32474697	11	109	dep	therapies	2458:2466	arg1	prevent					2483:2489	prevent	2483:2489	prevent cartilage degeneration by influencing the loss of mineralized bone	2483:2556	As a clinical conclusion, therapies to postpone or prevent cartilage degeneration by influencing the loss of mineralized bone could be site dependent.
32474697	11	109	dep	therapies	2458:2466	arg1	postpone					2471:2478	postpone	2471:2478	postpone	2471:2478	As a clinical conclusion, therapies to postpone or prevent cartilage degeneration by influencing the loss of mineralized bone could be site dependent.
32474697	0	110	theme	articular	93:101	arg1	cartilage					103:111	articular cartilage	93:111	articular cartilage	93:111	Varying development of femoral and tibial subchondral bone tissue and their interaction with articular cartilage during progressing osteoarthritis.
32474697	10	111	dep	CONCLUSIONS	2267:2277	arg1	showed					2327:2332	showed	2327:2332	showed varying developments and correlations among each other in the different compartments of the knee	2327:2429	CONCLUSIONS Structural bone and cartilage parameter changes showed varying developments and correlations among each other in the different compartments of the knee.
32474697	2	112	theme	key	706:708	arg1	molecules					710:718	key molecules	706:718	key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis)	706:857	MATERIALS AND METHODS Therefore, with the help of immunohistochemistry and real-time polymerase chain reaction (RT-PCR), human knee joint cartilage tissue was investigated for expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis).
32474697	5	113	theme	grade	1370:1374	arg1	lesions					1382:1388	ICRS grade 3a/3b lesions	1365:1388	ICRS grade 3a/3b lesions (group B)	1365:1398	From an enduring series of 108 patients undergoing implantation of TKA, 34 osteochondral samples with lesions macroscopically classified as ICRS grade 1b (group A) and 34 samples with ICRS grade 3a/3b lesions (group B) were compared with 21 healthy controls.
32474697	5	113	theme	grade	1370:1374	arg1	B					1397:1397	group B	1391:1397	group B	1391:1397	From an enduring series of 108 patients undergoing implantation of TKA, 34 osteochondral samples with lesions macroscopically classified as ICRS grade 1b (group A) and 34 samples with ICRS grade 3a/3b lesions (group B) were compared with 21 healthy controls.
32474697	6	114	theme	bone	1659:1662	arg1	resorption					1664:1673	subchondral bone resorption	1647:1673	subchondral bone resorption pits	1647:1678	RESULTS Concerning 3D analysis, the medial femoral condyle and tibia showed the most significant increase in bone volume fraction and a decrease in the trabecular number in group B frequently accompanied by subchondral bone resorption pits and enchondral ossification.
32474697	3	115	theme	high-resolution	968:982	arg1	micro-CT					984:991	high-resolution micro-CT	968:991	high-resolution micro-CT system	968:998	Furthermore, we correlated the results with 3D microcomputed tomography of the underlying subchondral bone (high-resolution micro-CT system).
32474697	1	116	dep	examined	338:345	arg1	although					348:355	although	348:355	although	348:355	INTRODUCTION Differences between tibial and femoral joint surfaces and knee compartments concerning coupled bone and cartilage turnover or bone-cartilage cross talk have not been previously examined, although the mechanical and biological interaction of the mineralized subchondral tissues with articular cartilage is of great importance for advancing osteoarthritis.
32474697	10	117	theme	parameter	2309:2317	arg1	changes					2319:2325	Structural bone and cartilage parameter changes	2279:2325	Structural bone and cartilage parameter changes	2279:2325	CONCLUSIONS Structural bone and cartilage parameter changes showed varying developments and correlations among each other in the different compartments of the knee.
32474697	1	118	theme	subchondral	418:428	arg1	tissues					430:436	the mineralized subchondral tissues	402:436	the mineralized subchondral tissues	402:436	INTRODUCTION Differences between tibial and femoral joint surfaces and knee compartments concerning coupled bone and cartilage turnover or bone-cartilage cross talk have not been previously examined, although the mechanical and biological interaction of the mineralized subchondral tissues with articular cartilage is of great importance for advancing osteoarthritis.
32474697	6	119	from	increase	1537:1544	arg1	fraction					1561:1568	bone volume fraction	1549:1568	bone volume fraction	1549:1568	RESULTS Concerning 3D analysis, the medial femoral condyle and tibia showed the most significant increase in bone volume fraction and a decrease in the trabecular number in group B frequently accompanied by subchondral bone resorption pits and enchondral ossification.
32474697	6	119	from	increase	1537:1544	arg1	number					1603:1608	the trabecular number	1588:1608	the trabecular number in group B frequently accompanied by subchondral bone resorption pits and enchondral ossification	1588:1706	RESULTS Concerning 3D analysis, the medial femoral condyle and tibia showed the most significant increase in bone volume fraction and a decrease in the trabecular number in group B frequently accompanied by subchondral bone resorption pits and enchondral ossification.
32474697	3	120	theme	bone	962:965	arg1	tomography					921:930	3D microcomputed tomography	904:930	3D microcomputed tomography of the underlying subchondral bone (high-resolution micro-CT system)	904:999	Furthermore, we correlated the results with 3D microcomputed tomography of the underlying subchondral bone (high-resolution micro-CT system).
32474697	2	121	theme	extracellular	727:739	arg1	matrix					741:746	the extracellular matrix	723:746	the extracellular matrix	723:746	MATERIALS AND METHODS Therefore, with the help of immunohistochemistry and real-time polymerase chain reaction (RT-PCR), human knee joint cartilage tissue was investigated for expression of key molecules of the extracellular matrix and cartilage composition (collagen type I and II, aggrecan) plus proteoglycan content (colorimetric analysis).
32474697	5	122	theme	34	1349:1350	arg1	samples					1352:1358	34 samples	1349:1358	34 samples with ICRS grade 3a/3b lesions (group B)	1349:1398	From an enduring series of 108 patients undergoing implantation of TKA, 34 osteochondral samples with lesions macroscopically classified as ICRS grade 1b (group A) and 34 samples with ICRS grade 3a/3b lesions (group B) were compared with 21 healthy controls.
32474697	0	123	theme	femoral	23:29	arg1	tissue					59:64	femoral and tibial subchondral bone tissue	23:64	femoral and tibial subchondral bone tissue	23:64	Varying development of femoral and tibial subchondral bone tissue and their interaction with articular cartilage during progressing osteoarthritis.
32474697	0	124	theme	tibial	35:40	arg1	tissue					59:64	femoral and tibial subchondral bone tissue	23:64	femoral and tibial subchondral bone tissue	23:64	Varying development of femoral and tibial subchondral bone tissue and their interaction with articular cartilage during progressing osteoarthritis.
32474697	7	125	theme	tibia	1741:1745	arg1	plateaus					1747:1754	tibia plateaus	1741:1754	tibia plateaus	1741:1754	Under physiological conditions, tibia plateaus show lower bone volume fraction than the corresponding femoral site and this difference enlarges with advancing OA.
32474697	4	126	theme	site	1062:1065	arg1	dependence					1033:1042	dependence	1033:1042	dependence of the anatomical site (femoral vs. tibial, medial and lateral each)	1033:1111	Measurements were performed in dependence of the anatomical site (femoral vs. tibial, medial and lateral each) to identify regional differences during the osteoarthritic process.
32474697	9	127	theme	varying	2095:2101	arg1	changes					2103:2109	faster and varying changes	2084:2109	faster and varying changes than type I	2084:2121	Collagen type II expression levels show faster and varying changes than type I during the OA process, leading to a lower positive or negative correlation with bone microstructural analysis, especially on the tibia plateau.
32474697	5	128	with	samples	1270:1276	arg1	lesions					1382:1388	ICRS grade 3a/3b lesions	1365:1388	ICRS grade 3a/3b lesions (group B)	1365:1398	From an enduring series of 108 patients undergoing implantation of TKA, 34 osteochondral samples with lesions macroscopically classified as ICRS grade 1b (group A) and 34 samples with ICRS grade 3a/3b lesions (group B) were compared with 21 healthy controls.
32474697	5	128	with	samples	1270:1276	arg1	B					1397:1397	group B	1391:1397	group B	1391:1397	From an enduring series of 108 patients undergoing implantation of TKA, 34 osteochondral samples with lesions macroscopically classified as ICRS grade 1b (group A) and 34 samples with ICRS grade 3a/3b lesions (group B) were compared with 21 healthy controls.
32474697	5	128	with	samples	1270:1276	arg1	lesions					1283:1289	lesions	1283:1289	lesions macroscopically classified as ICRS grade 1b (group A)	1283:1343	From an enduring series of 108 patients undergoing implantation of TKA, 34 osteochondral samples with lesions macroscopically classified as ICRS grade 1b (group A) and 34 samples with ICRS grade 3a/3b lesions (group B) were compared with 21 healthy controls.
32474697	0	129	theme	bone	54:57	arg1	tissue					59:64	femoral and tibial subchondral bone tissue	23:64	femoral and tibial subchondral bone tissue	23:64	Varying development of femoral and tibial subchondral bone tissue and their interaction with articular cartilage during progressing osteoarthritis.
32474697	8	130	theme	medial	1979:1984	arg1	plateau					1986:1992	the lateral tibial and medial plateau	1956:1992	plateau	1986:1992	Partially even contradictory behavior was observed such as trabecular separation at the lateral tibial and medial plateau in osteochondral OA samples of the same patients.
32474697	9	131	theme	OA	2134:2135	arg1	process					2137:2143	the OA process	2130:2143	the OA process	2130:2143	Collagen type II expression levels show faster and varying changes than type I during the OA process, leading to a lower positive or negative correlation with bone microstructural analysis, especially on the tibia plateau.
32474697	0	132	with	development	8:18	arg1	cartilage					103:111	articular cartilage	93:111	articular cartilage	93:111	Varying development of femoral and tibial subchondral bone tissue and their interaction with articular cartilage during progressing osteoarthritis.
32474697	6	133	theme	subchondral	1647:1657	arg1	resorption					1664:1673	subchondral bone resorption	1647:1673	subchondral bone resorption pits	1647:1678	RESULTS Concerning 3D analysis, the medial femoral condyle and tibia showed the most significant increase in bone volume fraction and a decrease in the trabecular number in group B frequently accompanied by subchondral bone resorption pits and enchondral ossification.
32474697	8	134	from	plateau	1986:1992	arg1	separation					1942:1951	trabecular separation	1931:1951	trabecular separation at the lateral tibial and medial plateau in osteochondral OA samples of the same patients	1931:2041	Partially even contradictory behavior was observed such as trabecular separation at the lateral tibial and medial plateau in osteochondral OA samples of the same patients.
32474697	5	135	theme	group	1391:1395	arg1	lesions					1382:1388	ICRS grade 3a/3b lesions	1365:1388	ICRS grade 3a/3b lesions (group B)	1365:1398	From an enduring series of 108 patients undergoing implantation of TKA, 34 osteochondral samples with lesions macroscopically classified as ICRS grade 1b (group A) and 34 samples with ICRS grade 3a/3b lesions (group B) were compared with 21 healthy controls.
32474697	5	135	theme	group	1391:1395	arg1	B					1397:1397	group B	1391:1397	group B	1391:1397	From an enduring series of 108 patients undergoing implantation of TKA, 34 osteochondral samples with lesions macroscopically classified as ICRS grade 1b (group A) and 34 samples with ICRS grade 3a/3b lesions (group B) were compared with 21 healthy controls.
32474697	7	136	theme	physiological	1715:1727	arg1	conditions					1729:1738	physiological conditions	1715:1738	physiological conditions	1715:1738	Under physiological conditions, tibia plateaus show lower bone volume fraction than the corresponding femoral site and this difference enlarges with advancing OA.
31819985	0	0	theme	Natural	75:81	arg1	Cells					92:96	Invariant Natural Killer T Cells	65:96	Invariant Natural Killer T Cells	65:96	Fecal IgA Levels and Gut Microbiota Composition Are Regulated by Invariant Natural Killer T Cells.
31819985	6	1	theme	cells	853:857	arg1	absence					837:843	The absence	833:843	The absence of iNKT cells	833:857	The absence of iNKT cells also affected TGF-β1 levels and plasma cells, which were significantly reduced in knockout (KO) mice.
31819985	1	2	theme	key	134:136	arg1	microbiota					118:127	The gut microbiota	110:127	The gut microbiota	110:127	BACKGROUND The gut microbiota is a key element to support host homeostasis and the development of the immune system.
31819985	1	2	theme	key	134:136	arg1	element					138:144	a key element	132:144	a key element to support host homeostasis and the development of the immune system	132:213	BACKGROUND The gut microbiota is a key element to support host homeostasis and the development of the immune system.
31819985	6	3	theme	plasma	891:896	arg1	cells					898:902	plasma cells	891:902	plasma cells	891:902	The absence of iNKT cells also affected TGF-β1 levels and plasma cells, which were significantly reduced in knockout (KO) mice.
31819985	0	4	theme	Invariant	65:73	arg1	Cells					92:96	Invariant Natural Killer T Cells	65:96	Invariant Natural Killer T Cells	65:96	Fecal IgA Levels and Gut Microbiota Composition Are Regulated by Invariant Natural Killer T Cells.
31819985	7	5	theme	dextran	992:998	arg1	sulfate					1007:1013	dextran sodium sulfate	992:1013	dextran sodium sulfate colitis	992:1021	In addition, when submitted to dextran sodium sulfate colitis, iNKT-KO mice had worsening of colitis when compared with wild-type (WT) mice.
31819985	5	6	theme	gut	787:789	arg1	microbiota					791:800	the gut microbiota	783:800	the gut microbiota	783:800	RESULTS We found that iNKT-deficient mice (iNKT-KO) had reduced levels of fecal IgA and an altered composition of the gut microbiota, with increased Bacteroidetes.
31819985	3	7	theme	NKT	460:462	arg1	cells					453:457	natural killer T cells	436:457	natural killer T cells (NKT cells)	436:469	In this context, natural killer T cells (NKT cells) are distinct T lymphocytes that play a role in gut immunity and are influenced by gut microbes.
31819985	3	7	theme	NKT	460:462	arg1	cells					464:468	NKT cells	460:468	NKT cells	460:468	In this context, natural killer T cells (NKT cells) are distinct T lymphocytes that play a role in gut immunity and are influenced by gut microbes.
31819985	4	8	from	involvement	600:610	arg1	homeostasis					656:666	intestinal homeostasis	645:666	intestinal homeostasis	645:666	In our work, we investigated the involvement of invariant NKT cells (iNKT) in intestinal homeostasis.
31819985	10	9	theme	bowel	1552:1556	arg1	diseases					1558:1565	inflammatory bowel diseases	1539:1565	inflammatory bowel diseases	1539:1565	CONCLUSIONS Our results indicate that intestinal NKT cells are important modulators of intestinal homeostasis and that gut microbiota composition may be a potential target in the management of inflammatory bowel diseases.
31819985	0	10	theme	T	90:90	arg1	Cells					92:96	Invariant Natural Killer T Cells	65:96	Invariant Natural Killer T Cells	65:96	Fecal IgA Levels and Gut Microbiota Composition Are Regulated by Invariant Natural Killer T Cells.
31819985	3	11	theme	T	484:484	arg1	lymphocytes					486:496	distinct T lymphocytes	475:496	distinct T lymphocytes that play a role in gut immunity and are influenced by gut microbes	475:564	In this context, natural killer T cells (NKT cells) are distinct T lymphocytes that play a role in gut immunity and are influenced by gut microbes.
31819985	3	11	theme	T	484:484	arg1	cells					453:457	natural killer T cells	436:457	natural killer T cells (NKT cells)	436:469	In this context, natural killer T cells (NKT cells) are distinct T lymphocytes that play a role in gut immunity and are influenced by gut microbes.
31819985	3	12	from	lymphocytes	486:496	arg1	context					427:433	this context	422:433	this context	422:433	In this context, natural killer T cells (NKT cells) are distinct T lymphocytes that play a role in gut immunity and are influenced by gut microbes.
31819985	10	13	theme	diseases	1558:1565	arg1	management					1525:1534	the management	1521:1534	the management of inflammatory bowel diseases	1521:1565	CONCLUSIONS Our results indicate that intestinal NKT cells are important modulators of intestinal homeostasis and that gut microbiota composition may be a potential target in the management of inflammatory bowel diseases.
31819985	0	14	theme	Killer	83:88	arg1	Cells					92:96	Invariant Natural Killer T Cells	65:96	Invariant Natural Killer T Cells	65:96	Fecal IgA Levels and Gut Microbiota Composition Are Regulated by Invariant Natural Killer T Cells.
31819985	8	15	theme	iNKT	1197:1200	arg1	cells					1202:1206	iNKT cells	1197:1206	iNKT cells	1197:1206	To further address iNKT cell contribution to intestinal homeostasis, we adoptively transferred iNKT cells to KO mice, and they were submitted to colitis.
31819985	10	16	theme	microbiota	1469:1478	arg1	target					1511:1516	a potential target	1499:1516	a potential target in the management of inflammatory bowel diseases	1499:1565	CONCLUSIONS Our results indicate that intestinal NKT cells are important modulators of intestinal homeostasis and that gut microbiota composition may be a potential target in the management of inflammatory bowel diseases.
31819985	10	16	theme	microbiota	1469:1478	arg1	composition					1480:1490	gut microbiota composition	1465:1490	gut microbiota composition	1465:1490	CONCLUSIONS Our results indicate that intestinal NKT cells are important modulators of intestinal homeostasis and that gut microbiota composition may be a potential target in the management of inflammatory bowel diseases.
31819985	4	17	theme	invariant	615:623	arg1	cells					629:633	invariant NKT cells	615:633	invariant NKT cells (iNKT)	615:640	In our work, we investigated the involvement of invariant NKT cells (iNKT) in intestinal homeostasis.
31819985	4	17	theme	invariant	615:623	arg1	iNKT					636:639	iNKT	636:639	iNKT	636:639	In our work, we investigated the involvement of invariant NKT cells (iNKT) in intestinal homeostasis.
31819985	6	18	theme	TGF-β1	873:878	arg1	levels					880:885	TGF-β1 levels	873:885	TGF-β1 levels	873:885	The absence of iNKT cells also affected TGF-β1 levels and plasma cells, which were significantly reduced in knockout (KO) mice.
31819985	10	19	theme	important	1409:1417	arg1	cells					1399:1403	intestinal NKT cells	1384:1403	intestinal NKT cells	1384:1403	CONCLUSIONS Our results indicate that intestinal NKT cells are important modulators of intestinal homeostasis and that gut microbiota composition may be a potential target in the management of inflammatory bowel diseases.
31819985	10	19	theme	important	1409:1417	arg1	modulators					1419:1428	important modulators	1409:1428	important modulators of intestinal homeostasis	1409:1454	CONCLUSIONS Our results indicate that intestinal NKT cells are important modulators of intestinal homeostasis and that gut microbiota composition may be a potential target in the management of inflammatory bowel diseases.
31819985	4	20	theme	cells	629:633	arg1	involvement					600:610	the involvement	596:610	the involvement of invariant NKT cells (iNKT) in intestinal homeostasis	596:666	In our work, we investigated the involvement of invariant NKT cells (iNKT) in intestinal homeostasis.
31819985	1	21	dep	BACKGROUND	99:108	arg1	microbiota					118:127	The gut microbiota	110:127	The gut microbiota	110:127	BACKGROUND The gut microbiota is a key element to support host homeostasis and the development of the immune system.
31819985	1	21	dep	BACKGROUND	99:108	arg1	element					138:144	a key element	132:144	a key element to support host homeostasis and the development of the immune system	132:213	BACKGROUND The gut microbiota is a key element to support host homeostasis and the development of the immune system.
31819985	2	22	theme	immune	353:358	arg1	cells					360:364	immune cells	353:364	immune cells	353:364	The relationship between the microbiota and immunity is a 2-way road, in which the microbiota contributes to the development/function of immune cells and immunity can affect the composition of microbes.
31819985	9	23	theme	cells	1273:1277	arg1	Transfer					1256:1263	Transfer	1256:1263	Transfer of iNKT cells	1256:1277	Transfer of iNKT cells improved colitis and restored fecal IgA levels and gut microbiota.
31819985	7	24	theme	sulfate	1007:1013	arg1	colitis					1015:1021	dextran sodium sulfate colitis	992:1021	dextran sodium sulfate colitis	992:1021	In addition, when submitted to dextran sodium sulfate colitis, iNKT-KO mice had worsening of colitis when compared with wild-type (WT) mice.
31819985	5	25	theme	microbiota	791:800	arg1	levels					733:738	reduced levels	725:738	reduced levels of fecal IgA	725:751	RESULTS We found that iNKT-deficient mice (iNKT-KO) had reduced levels of fecal IgA and an altered composition of the gut microbiota, with increased Bacteroidetes.
31819985	5	25	theme	microbiota	791:800	arg1	composition					768:778	an altered composition	757:778	an altered composition of the gut microbiota	757:800	RESULTS We found that iNKT-deficient mice (iNKT-KO) had reduced levels of fecal IgA and an altered composition of the gut microbiota, with increased Bacteroidetes.
31819985	2	26	theme	microbes	409:416	arg1	composition					394:404	the composition	390:404	the composition of microbes	390:416	The relationship between the microbiota and immunity is a 2-way road, in which the microbiota contributes to the development/function of immune cells and immunity can affect the composition of microbes.
31819985	2	27	theme	cells	360:364	arg1	development/function					329:348	the development/function	325:348	the development/function of immune cells	325:364	The relationship between the microbiota and immunity is a 2-way road, in which the microbiota contributes to the development/function of immune cells and immunity can affect the composition of microbes.
31819985	0	28	theme	IgA	6:8	arg1	Levels					10:15	Fecal IgA Levels	0:15	Fecal IgA Levels	0:15	Fecal IgA Levels and Gut Microbiota Composition Are Regulated by Invariant Natural Killer T Cells.
31819985	7	29	theme	wild-type	1081:1089	arg1	mice					1096:1099	wild-type (WT) mice	1081:1099	wild-type (WT) mice	1081:1099	In addition, when submitted to dextran sodium sulfate colitis, iNKT-KO mice had worsening of colitis when compared with wild-type (WT) mice.
31819985	6	30	theme	KO	951:952	arg1	mice					955:958	knockout (KO) mice	941:958	knockout (KO) mice	941:958	The absence of iNKT cells also affected TGF-β1 levels and plasma cells, which were significantly reduced in knockout (KO) mice.
31819985	1	31	theme	host	157:160	arg1	homeostasis					162:172	host homeostasis	157:172	host homeostasis	157:172	BACKGROUND The gut microbiota is a key element to support host homeostasis and the development of the immune system.
31819985	0	32	theme	Fecal	0:4	arg1	Levels					10:15	Fecal IgA Levels	0:15	Fecal IgA Levels	0:15	Fecal IgA Levels and Gut Microbiota Composition Are Regulated by Invariant Natural Killer T Cells.
31819985	10	33	theme	gut	1465:1467	arg1	target					1511:1516	a potential target	1499:1516	a potential target in the management of inflammatory bowel diseases	1499:1565	CONCLUSIONS Our results indicate that intestinal NKT cells are important modulators of intestinal homeostasis and that gut microbiota composition may be a potential target in the management of inflammatory bowel diseases.
31819985	10	33	theme	gut	1465:1467	arg1	composition					1480:1490	gut microbiota composition	1465:1490	gut microbiota composition	1465:1490	CONCLUSIONS Our results indicate that intestinal NKT cells are important modulators of intestinal homeostasis and that gut microbiota composition may be a potential target in the management of inflammatory bowel diseases.
31819985	10	34	from	modulators	1419:1428	arg1	management					1525:1534	the management	1521:1534	the management of inflammatory bowel diseases	1521:1565	CONCLUSIONS Our results indicate that intestinal NKT cells are important modulators of intestinal homeostasis and that gut microbiota composition may be a potential target in the management of inflammatory bowel diseases.
31819985	10	35	theme	inflammatory	1539:1550	arg1	diseases					1558:1565	inflammatory bowel diseases	1539:1565	inflammatory bowel diseases	1539:1565	CONCLUSIONS Our results indicate that intestinal NKT cells are important modulators of intestinal homeostasis and that gut microbiota composition may be a potential target in the management of inflammatory bowel diseases.
31819985	8	36	theme	KO	1211:1212	arg1	mice					1214:1217	KO mice	1211:1217	KO mice	1211:1217	To further address iNKT cell contribution to intestinal homeostasis, we adoptively transferred iNKT cells to KO mice, and they were submitted to colitis.
31819985	7	37	theme	iNKT-KO	1024:1030	arg1	mice					1032:1035	iNKT-KO mice	1024:1035	iNKT-KO mice	1024:1035	In addition, when submitted to dextran sodium sulfate colitis, iNKT-KO mice had worsening of colitis when compared with wild-type (WT) mice.
31819985	8	38	theme	intestinal	1147:1156	arg1	homeostasis					1158:1168	intestinal homeostasis	1147:1168	intestinal homeostasis	1147:1168	To further address iNKT cell contribution to intestinal homeostasis, we adoptively transferred iNKT cells to KO mice, and they were submitted to colitis.
31819985	7	39	contain	had	1037:1039	arg2	worsening					1041:1049	worsening	1041:1049	worsening of colitis	1041:1060	In addition, when submitted to dextran sodium sulfate colitis, iNKT-KO mice had worsening of colitis when compared with wild-type (WT) mice.
31819985	7	39	contain	had	1037:1039	arg1	mice					1032:1035	iNKT-KO mice	1024:1035	iNKT-KO mice	1024:1035	In addition, when submitted to dextran sodium sulfate colitis, iNKT-KO mice had worsening of colitis when compared with wild-type (WT) mice.
31819985	2	40	theme	2-way	274:278	arg1	relationship					220:231	The relationship	216:231	The relationship between the microbiota and immunity	216:267	The relationship between the microbiota and immunity is a 2-way road, in which the microbiota contributes to the development/function of immune cells and immunity can affect the composition of microbes.
31819985	2	40	theme	2-way	274:278	arg1	road					280:283	a 2-way road	272:283	a 2-way road	272:283	The relationship between the microbiota and immunity is a 2-way road, in which the microbiota contributes to the development/function of immune cells and immunity can affect the composition of microbes.
31819985	6	41	theme	knockout	941:948	arg1	mice					955:958	knockout (KO) mice	941:958	knockout (KO) mice	941:958	The absence of iNKT cells also affected TGF-β1 levels and plasma cells, which were significantly reduced in knockout (KO) mice.
31819985	5	42	theme	reduced	725:731	arg1	levels					733:738	reduced levels	725:738	reduced levels of fecal IgA	725:751	RESULTS We found that iNKT-deficient mice (iNKT-KO) had reduced levels of fecal IgA and an altered composition of the gut microbiota, with increased Bacteroidetes.
31819985	5	43	theme	increased	808:816	arg1	Bacteroidetes					818:830	increased Bacteroidetes	808:830	increased Bacteroidetes	808:830	RESULTS We found that iNKT-deficient mice (iNKT-KO) had reduced levels of fecal IgA and an altered composition of the gut microbiota, with increased Bacteroidetes.
31819985	7	44	dep	wild-type	1081:1089	arg1	WT					1092:1093	WT	1092:1093	WT	1092:1093	In addition, when submitted to dextran sodium sulfate colitis, iNKT-KO mice had worsening of colitis when compared with wild-type (WT) mice.
31819985	4	45	theme	intestinal	645:654	arg1	homeostasis					656:666	intestinal homeostasis	645:666	intestinal homeostasis	645:666	In our work, we investigated the involvement of invariant NKT cells (iNKT) in intestinal homeostasis.
31819985	9	46	theme	iNKT	1268:1271	arg1	cells					1273:1277	iNKT cells	1268:1277	iNKT cells	1268:1277	Transfer of iNKT cells improved colitis and restored fecal IgA levels and gut microbiota.
31819985	9	47	theme	fecal	1309:1313	arg1	levels					1319:1324	fecal IgA levels	1309:1324	fecal IgA levels	1309:1324	Transfer of iNKT cells improved colitis and restored fecal IgA levels and gut microbiota.
31819985	0	48	theme	Microbiota	25:34	arg1	Composition					36:46	Gut Microbiota Composition	21:46	Gut Microbiota Composition	21:46	Fecal IgA Levels and Gut Microbiota Composition Are Regulated by Invariant Natural Killer T Cells.
31819985	6	49	theme	iNKT	848:851	arg1	cells					853:857	iNKT cells	848:857	iNKT cells	848:857	The absence of iNKT cells also affected TGF-β1 levels and plasma cells, which were significantly reduced in knockout (KO) mice.
31819985	5	50	contain	had	721:723	arg1	mice					706:709	iNKT-deficient mice	691:709	iNKT-deficient mice (iNKT-KO)	691:719	RESULTS We found that iNKT-deficient mice (iNKT-KO) had reduced levels of fecal IgA and an altered composition of the gut microbiota, with increased Bacteroidetes.
31819985	5	50	contain	had	721:723	arg2	composition					768:778	an altered composition	757:778	an altered composition of the gut microbiota	757:800	RESULTS We found that iNKT-deficient mice (iNKT-KO) had reduced levels of fecal IgA and an altered composition of the gut microbiota, with increased Bacteroidetes.
31819985	5	50	contain	had	721:723	arg1	iNKT-KO					712:718	iNKT-KO	712:718	iNKT-KO	712:718	RESULTS We found that iNKT-deficient mice (iNKT-KO) had reduced levels of fecal IgA and an altered composition of the gut microbiota, with increased Bacteroidetes.
31819985	5	50	contain	had	721:723	arg2	levels					733:738	reduced levels	725:738	reduced levels of fecal IgA	725:751	RESULTS We found that iNKT-deficient mice (iNKT-KO) had reduced levels of fecal IgA and an altered composition of the gut microbiota, with increased Bacteroidetes.
31819985	10	51	theme	homeostasis	1444:1454	arg1	composition					1480:1490	gut microbiota composition	1465:1490	gut microbiota composition	1465:1490	CONCLUSIONS Our results indicate that intestinal NKT cells are important modulators of intestinal homeostasis and that gut microbiota composition may be a potential target in the management of inflammatory bowel diseases.
31819985	10	51	theme	homeostasis	1444:1454	arg1	cells					1399:1403	intestinal NKT cells	1384:1403	intestinal NKT cells	1384:1403	CONCLUSIONS Our results indicate that intestinal NKT cells are important modulators of intestinal homeostasis and that gut microbiota composition may be a potential target in the management of inflammatory bowel diseases.
31819985	10	51	theme	homeostasis	1444:1454	arg1	target					1511:1516	a potential target	1499:1516	a potential target in the management of inflammatory bowel diseases	1499:1565	CONCLUSIONS Our results indicate that intestinal NKT cells are important modulators of intestinal homeostasis and that gut microbiota composition may be a potential target in the management of inflammatory bowel diseases.
31819985	10	51	theme	homeostasis	1444:1454	arg1	modulators					1419:1428	important modulators	1409:1428	important modulators of intestinal homeostasis	1409:1454	CONCLUSIONS Our results indicate that intestinal NKT cells are important modulators of intestinal homeostasis and that gut microbiota composition may be a potential target in the management of inflammatory bowel diseases.
31819985	5	52	theme	fecal	743:747	arg1	IgA					749:751	fecal IgA	743:751	fecal IgA	743:751	RESULTS We found that iNKT-deficient mice (iNKT-KO) had reduced levels of fecal IgA and an altered composition of the gut microbiota, with increased Bacteroidetes.
31819985	0	53	theme	Gut	21:23	arg1	Composition					36:46	Gut Microbiota Composition	21:46	Gut Microbiota Composition	21:46	Fecal IgA Levels and Gut Microbiota Composition Are Regulated by Invariant Natural Killer T Cells.
31819985	5	54	theme	iNKT-deficient	691:704	arg1	mice					706:709	iNKT-deficient mice	691:709	iNKT-deficient mice (iNKT-KO)	691:719	RESULTS We found that iNKT-deficient mice (iNKT-KO) had reduced levels of fecal IgA and an altered composition of the gut microbiota, with increased Bacteroidetes.
31819985	5	54	theme	iNKT-deficient	691:704	arg1	iNKT-KO					712:718	iNKT-KO	712:718	iNKT-KO	712:718	RESULTS We found that iNKT-deficient mice (iNKT-KO) had reduced levels of fecal IgA and an altered composition of the gut microbiota, with increased Bacteroidetes.
31819985	8	55	theme	cell	1126:1129	arg1	contribution					1131:1142	iNKT cell contribution	1121:1142	iNKT cell contribution to intestinal homeostasis	1121:1168	To further address iNKT cell contribution to intestinal homeostasis, we adoptively transferred iNKT cells to KO mice, and they were submitted to colitis.
31819985	7	56	theme	colitis	1054:1060	arg1	worsening					1041:1049	worsening	1041:1049	worsening of colitis	1041:1060	In addition, when submitted to dextran sodium sulfate colitis, iNKT-KO mice had worsening of colitis when compared with wild-type (WT) mice.
31819985	5	57	theme	IgA	749:751	arg1	levels					733:738	reduced levels	725:738	reduced levels of fecal IgA	725:751	RESULTS We found that iNKT-deficient mice (iNKT-KO) had reduced levels of fecal IgA and an altered composition of the gut microbiota, with increased Bacteroidetes.
31819985	5	57	theme	IgA	749:751	arg1	composition					768:778	an altered composition	757:778	an altered composition of the gut microbiota	757:800	RESULTS We found that iNKT-deficient mice (iNKT-KO) had reduced levels of fecal IgA and an altered composition of the gut microbiota, with increased Bacteroidetes.
31819985	7	58	theme	sodium	1000:1005	arg1	sulfate					1007:1013	dextran sodium sulfate	992:1013	dextran sodium sulfate colitis	992:1021	In addition, when submitted to dextran sodium sulfate colitis, iNKT-KO mice had worsening of colitis when compared with wild-type (WT) mice.
31819985	5	59	theme	altered	760:766	arg1	composition					768:778	an altered composition	757:778	an altered composition of the gut microbiota	757:800	RESULTS We found that iNKT-deficient mice (iNKT-KO) had reduced levels of fecal IgA and an altered composition of the gut microbiota, with increased Bacteroidetes.
31819985	10	60	dep	CONCLUSIONS	1346:1356	arg1	indicate					1370:1377	indicate	1370:1377	indicate that intestinal NKT cells are important modulators of intestinal homeostasis and that gut microbiota composition may be a potential target in the management of inflammatory bowel diseases	1370:1565	CONCLUSIONS Our results indicate that intestinal NKT cells are important modulators of intestinal homeostasis and that gut microbiota composition may be a potential target in the management of inflammatory bowel diseases.
31819985	9	61	theme	IgA	1315:1317	arg1	levels					1319:1324	fecal IgA levels	1309:1324	fecal IgA levels	1309:1324	Transfer of iNKT cells improved colitis and restored fecal IgA levels and gut microbiota.
31819985	3	62	theme	T	451:451	arg1	lymphocytes					486:496	distinct T lymphocytes	475:496	distinct T lymphocytes that play a role in gut immunity and are influenced by gut microbes	475:564	In this context, natural killer T cells (NKT cells) are distinct T lymphocytes that play a role in gut immunity and are influenced by gut microbes.
31819985	3	62	theme	T	451:451	arg1	cells					453:457	natural killer T cells	436:457	natural killer T cells (NKT cells)	436:469	In this context, natural killer T cells (NKT cells) are distinct T lymphocytes that play a role in gut immunity and are influenced by gut microbes.
31819985	3	62	theme	T	451:451	arg1	cells					464:468	NKT cells	460:468	NKT cells	460:468	In this context, natural killer T cells (NKT cells) are distinct T lymphocytes that play a role in gut immunity and are influenced by gut microbes.
31819985	5	63	dep	RESULTS	669:675	arg1	found					680:684	found	680:684	found that iNKT-deficient mice (iNKT-KO) had reduced levels of fecal IgA and an altered composition of the gut microbiota, with increased Bacteroidetes	680:830	RESULTS We found that iNKT-deficient mice (iNKT-KO) had reduced levels of fecal IgA and an altered composition of the gut microbiota, with increased Bacteroidetes.
31819985	3	64	theme	natural	436:442	arg1	lymphocytes					486:496	distinct T lymphocytes	475:496	distinct T lymphocytes that play a role in gut immunity and are influenced by gut microbes	475:564	In this context, natural killer T cells (NKT cells) are distinct T lymphocytes that play a role in gut immunity and are influenced by gut microbes.
31819985	3	64	theme	natural	436:442	arg1	cells					453:457	natural killer T cells	436:457	natural killer T cells (NKT cells)	436:469	In this context, natural killer T cells (NKT cells) are distinct T lymphocytes that play a role in gut immunity and are influenced by gut microbes.
31819985	3	64	theme	natural	436:442	arg1	cells					464:468	NKT cells	460:468	NKT cells	460:468	In this context, natural killer T cells (NKT cells) are distinct T lymphocytes that play a role in gut immunity and are influenced by gut microbes.
31819985	10	65	theme	intestinal	1384:1393	arg1	cells					1399:1403	intestinal NKT cells	1384:1403	intestinal NKT cells	1384:1403	CONCLUSIONS Our results indicate that intestinal NKT cells are important modulators of intestinal homeostasis and that gut microbiota composition may be a potential target in the management of inflammatory bowel diseases.
31819985	10	65	theme	intestinal	1384:1393	arg1	modulators					1419:1428	important modulators	1409:1428	important modulators of intestinal homeostasis	1409:1454	CONCLUSIONS Our results indicate that intestinal NKT cells are important modulators of intestinal homeostasis and that gut microbiota composition may be a potential target in the management of inflammatory bowel diseases.
31819985	3	66	theme	gut	518:520	arg1	immunity					522:529	gut immunity	518:529	gut immunity	518:529	In this context, natural killer T cells (NKT cells) are distinct T lymphocytes that play a role in gut immunity and are influenced by gut microbes.
31819985	10	67	from	target	1511:1516	arg1	management					1525:1534	the management	1521:1534	the management of inflammatory bowel diseases	1521:1565	CONCLUSIONS Our results indicate that intestinal NKT cells are important modulators of intestinal homeostasis and that gut microbiota composition may be a potential target in the management of inflammatory bowel diseases.
31819985	4	68	theme	NKT	625:627	arg1	cells					629:633	invariant NKT cells	615:633	invariant NKT cells (iNKT)	615:640	In our work, we investigated the involvement of invariant NKT cells (iNKT) in intestinal homeostasis.
31819985	4	68	theme	NKT	625:627	arg1	iNKT					636:639	iNKT	636:639	iNKT	636:639	In our work, we investigated the involvement of invariant NKT cells (iNKT) in intestinal homeostasis.
31819985	8	69	dep	adoptively	1174:1183	arg1	transferred					1185:1195	transferred	1185:1195	adoptively transferred iNKT cells to KO mice	1174:1217	To further address iNKT cell contribution to intestinal homeostasis, we adoptively transferred iNKT cells to KO mice, and they were submitted to colitis.
31819985	1	70	theme	gut	114:116	arg1	microbiota					118:127	The gut microbiota	110:127	The gut microbiota	110:127	BACKGROUND The gut microbiota is a key element to support host homeostasis and the development of the immune system.
31819985	1	70	theme	gut	114:116	arg1	element					138:144	a key element	132:144	a key element to support host homeostasis and the development of the immune system	132:213	BACKGROUND The gut microbiota is a key element to support host homeostasis and the development of the immune system.
31819985	3	71	theme	distinct	475:482	arg1	lymphocytes					486:496	distinct T lymphocytes	475:496	distinct T lymphocytes that play a role in gut immunity and are influenced by gut microbes	475:564	In this context, natural killer T cells (NKT cells) are distinct T lymphocytes that play a role in gut immunity and are influenced by gut microbes.
31819985	3	71	theme	distinct	475:482	arg1	cells					453:457	natural killer T cells	436:457	natural killer T cells (NKT cells)	436:469	In this context, natural killer T cells (NKT cells) are distinct T lymphocytes that play a role in gut immunity and are influenced by gut microbes.
31819985	3	72	theme	gut	553:555	arg1	microbes					557:564	gut microbes	553:564	gut microbes	553:564	In this context, natural killer T cells (NKT cells) are distinct T lymphocytes that play a role in gut immunity and are influenced by gut microbes.
31819985	3	73	theme	killer	444:449	arg1	lymphocytes					486:496	distinct T lymphocytes	475:496	distinct T lymphocytes that play a role in gut immunity and are influenced by gut microbes	475:564	In this context, natural killer T cells (NKT cells) are distinct T lymphocytes that play a role in gut immunity and are influenced by gut microbes.
31819985	3	73	theme	killer	444:449	arg1	cells					453:457	natural killer T cells	436:457	natural killer T cells (NKT cells)	436:469	In this context, natural killer T cells (NKT cells) are distinct T lymphocytes that play a role in gut immunity and are influenced by gut microbes.
31819985	3	73	theme	killer	444:449	arg1	cells					464:468	NKT cells	460:468	NKT cells	460:468	In this context, natural killer T cells (NKT cells) are distinct T lymphocytes that play a role in gut immunity and are influenced by gut microbes.
31819985	1	74	theme	immune	201:206	arg1	system					208:213	the immune system	197:213	the immune system	197:213	BACKGROUND The gut microbiota is a key element to support host homeostasis and the development of the immune system.
31819985	8	75	theme	iNKT	1121:1124	arg1	contribution					1131:1142	iNKT cell contribution	1121:1142	iNKT cell contribution to intestinal homeostasis	1121:1168	To further address iNKT cell contribution to intestinal homeostasis, we adoptively transferred iNKT cells to KO mice, and they were submitted to colitis.
31819985	9	76	theme	gut	1330:1332	arg1	microbiota					1334:1343	gut microbiota	1330:1343	gut microbiota	1330:1343	Transfer of iNKT cells improved colitis and restored fecal IgA levels and gut microbiota.
31819985	10	77	theme	potential	1501:1509	arg1	target					1511:1516	a potential target	1499:1516	a potential target in the management of inflammatory bowel diseases	1499:1565	CONCLUSIONS Our results indicate that intestinal NKT cells are important modulators of intestinal homeostasis and that gut microbiota composition may be a potential target in the management of inflammatory bowel diseases.
31819985	10	77	theme	potential	1501:1509	arg1	composition					1480:1490	gut microbiota composition	1465:1490	gut microbiota composition	1465:1490	CONCLUSIONS Our results indicate that intestinal NKT cells are important modulators of intestinal homeostasis and that gut microbiota composition may be a potential target in the management of inflammatory bowel diseases.
31819985	1	78	theme	system	208:213	arg1	homeostasis					162:172	host homeostasis	157:172	host homeostasis	157:172	BACKGROUND The gut microbiota is a key element to support host homeostasis and the development of the immune system.
31819985	1	78	theme	system	208:213	arg1	development					182:192	the development	178:192	the development of the immune system	178:213	BACKGROUND The gut microbiota is a key element to support host homeostasis and the development of the immune system.
31819985	10	79	theme	NKT	1395:1397	arg1	cells					1399:1403	intestinal NKT cells	1384:1403	intestinal NKT cells	1384:1403	CONCLUSIONS Our results indicate that intestinal NKT cells are important modulators of intestinal homeostasis and that gut microbiota composition may be a potential target in the management of inflammatory bowel diseases.
31819985	10	79	theme	NKT	1395:1397	arg1	modulators					1419:1428	important modulators	1409:1428	important modulators of intestinal homeostasis	1409:1454	CONCLUSIONS Our results indicate that intestinal NKT cells are important modulators of intestinal homeostasis and that gut microbiota composition may be a potential target in the management of inflammatory bowel diseases.
31819985	10	80	theme	intestinal	1433:1442	arg1	homeostasis					1444:1454	intestinal homeostasis	1433:1454	intestinal homeostasis	1433:1454	CONCLUSIONS Our results indicate that intestinal NKT cells are important modulators of intestinal homeostasis and that gut microbiota composition may be a potential target in the management of inflammatory bowel diseases.
31819985	2	81	dep	microbiota	245:254	arg1	the					241:243	the	241:243	the	241:243	The relationship between the microbiota and immunity is a 2-way road, in which the microbiota contributes to the development/function of immune cells and immunity can affect the composition of microbes.
34335640	0	0	theme	Wall	57:60	arg1	Composition					62:72	Cell Wall Composition	52:72	Cell Wall Composition	52:72	The Effect of Sugarcane Straw Aging in the Field on Cell Wall Composition.
34335640	6	1	theme	composition	824:834	arg1	determination					792:804	determination	792:804	determination of monosaccharide composition	792:834	The cell wall composition was determined by cell wall fractionation and determination of monosaccharide composition.
34335640	6	1	theme	composition	824:834	arg1	fractionation					774:786	cell wall fractionation	764:786	cell wall fractionation	764:786	The cell wall composition was determined by cell wall fractionation and determination of monosaccharide composition.
34335640	11	2	theme	compounds	1502:1510	arg1	level					1493:1497	the level	1489:1497	the level of compounds related to recalcitrance, which led to a reduction in saccharification and an increase in minerals and ash contents	1489:1626	The degradation and solubilization of the cell wall polysaccharides concomitantly increased the level of compounds related to recalcitrance, which led to a reduction in saccharification and an increase in minerals and ash contents.
34335640	9	3	from	Changes	1069:1075	arg1	compositions					1095:1106	monosaccharide compositions	1080:1106	monosaccharide compositions	1080:1106	Changes in monosaccharide compositions indicated solubilization of arabinoxylan (xylose and arabinose) and β-glucans (β-1,3 1,4 glucan; after 3 months) followed by degradation of cellulose (after 6 months).
34335640	9	4	dep	arabinoxylan	1136:1147	arg1	arabinose					1161:1169	arabinose	1161:1169	arabinose	1161:1169	Changes in monosaccharide compositions indicated solubilization of arabinoxylan (xylose and arabinose) and β-glucans (β-1,3 1,4 glucan; after 3 months) followed by degradation of cellulose (after 6 months).
34335640	9	4	dep	arabinoxylan	1136:1147	arg1	xylose					1150:1155	xylose	1150:1155	xylose	1150:1155	Changes in monosaccharide compositions indicated solubilization of arabinoxylan (xylose and arabinose) and β-glucans (β-1,3 1,4 glucan; after 3 months) followed by degradation of cellulose (after 6 months).
34335640	9	5	dep	β-glucans	1176:1184	arg1	glucan					1197:1202	β-1,3 1,4 glucan	1187:1202	β-1,3 1,4 glucan; after 3 months	1187:1218	Changes in monosaccharide compositions indicated solubilization of arabinoxylan (xylose and arabinose) and β-glucans (β-1,3 1,4 glucan; after 3 months) followed by degradation of cellulose (after 6 months).
34335640	0	6	theme	Cell	52:55	arg1	Composition					62:72	Cell Wall Composition	52:72	Cell Wall Composition	52:72	The Effect of Sugarcane Straw Aging in the Field on Cell Wall Composition.
34335640	0	7	from	Effect	4:9	arg1	Field					43:47	the Field	39:47	the Field	39:47	The Effect of Sugarcane Straw Aging in the Field on Cell Wall Composition.
34335640	0	7	from	Effect	4:9	arg1	Composition					62:72	Cell Wall Composition	52:72	Cell Wall Composition	52:72	The Effect of Sugarcane Straw Aging in the Field on Cell Wall Composition.
34335640	9	8	theme	β-glucans	1176:1184	arg1	solubilization					1118:1131	solubilization	1118:1131	solubilization of arabinoxylan (xylose and arabinose) and β-glucans (β-1,3 1,4 glucan; after 3 months) followed by degradation of cellulose (after 6 months)	1118:1273	Changes in monosaccharide compositions indicated solubilization of arabinoxylan (xylose and arabinose) and β-glucans (β-1,3 1,4 glucan; after 3 months) followed by degradation of cellulose (after 6 months).
34335640	1	9	from	amount	131:136	arg1	world					170:174	the world	166:174	the world	166:174	Cellulosic ethanol is an alternative for increasing the amount of bioethanol production in the world.
34335640	2	10	theme	possible	295:302	arg1	feedstock					304:312	a possible feedstock	293:312	a possible feedstock	293:312	In Brazil, sugarcane leads the bioethanol production, and to improve its yield, besides bagasse, sugarcane straw is a possible feedstock.
34335640	2	10	theme	possible	295:302	arg1	straw					284:288	sugarcane straw	274:288	sugarcane straw	274:288	In Brazil, sugarcane leads the bioethanol production, and to improve its yield, besides bagasse, sugarcane straw is a possible feedstock.
34335640	5	11	theme	Non-structural	611:624	arg1	carbohydrates					641:653	Non-structural and structural carbohydrates	611:653	Non-structural and structural carbohydrates	611:653	Non-structural and structural carbohydrates, lignin content, ash, and cellulose crystallinity were analyzed.
34335640	8	12	theme	plant	958:962	arg1	waste					964:968	the plant waste	954:968	the plant waste for up to 12 months	954:988	Pectins and lignin remained in the plant waste for up to 12 months, while the hemicelluloses and cellulose decreased 7.4 and 12.4%, respectively.
34335640	12	13	theme	Cellulose	1629:1637	arg1	crystallinity					1639:1651	Cellulose crystallinity	1629:1651	Cellulose crystallinity	1629:1651	Cellulose crystallinity changed little, with evidence of silica at the latter stages, indicating mineralization of the material.
34335640	12	14	theme	silica	1686:1691	arg1	evidence					1674:1681	evidence	1674:1681	evidence of silica at the latter stages	1674:1712	Cellulose crystallinity changed little, with evidence of silica at the latter stages, indicating mineralization of the material.
34335640	10	15	theme	xylose	1309:1314	arg1	ratio					1324:1328	the xylose:glucose ratio	1305:1328	the xylose:glucose ratio	1305:1328	Despite cellulose reduction, the xylose:glucose ratio increased, suggesting that glucose is consumed faster than xylose.
34335640	9	16	theme	monosaccharide	1080:1093	arg1	compositions					1095:1106	monosaccharide compositions	1080:1106	monosaccharide compositions	1080:1106	Changes in monosaccharide compositions indicated solubilization of arabinoxylan (xylose and arabinose) and β-glucans (β-1,3 1,4 glucan; after 3 months) followed by degradation of cellulose (after 6 months).
34335640	14	17	theme	bioenergy	1887:1895	arg1	purposes					1897:1904	bioenergy purposes	1887:1904	bioenergy purposes	1887:1904	Alternatively, for bioenergy purposes, straw should be used in less than 3 months.
34335640	5	18	theme	structural	630:639	arg1	carbohydrates					641:653	Non-structural and structural carbohydrates	611:653	Non-structural and structural carbohydrates	611:653	Non-structural and structural carbohydrates, lignin content, ash, and cellulose crystallinity were analyzed.
34335640	13	19	theme	soil	1791:1794	arg1	mineralization					1796:1809	better soil mineralization	1784:1809	better soil mineralization	1784:1809	Our data suggest that for better soil mineralization, sugarcane straw must stay in the field for over 1 year.
34335640	3	20	dep	this	429:432	arg1	happens					434:440	happens	434:440	happens	434:440	However, the process that leads to cell wall disassembly under field conditions is unknown, and understanding how this happens can improve sugarcane biorefinery and soil quality.
34335640	1	21	theme	bioethanol	141:150	arg1	production					152:161	bioethanol production	141:161	bioethanol production	141:161	Cellulosic ethanol is an alternative for increasing the amount of bioethanol production in the world.
34335640	8	22	dep	12	980:981	arg1	to					977:978	to	977:978	to	977:978	Pectins and lignin remained in the plant waste for up to 12 months, while the hemicelluloses and cellulose decreased 7.4 and 12.4%, respectively.
34335640	0	23	from	Aging	30:34	arg1	Field					43:47	the Field	39:47	the Field	39:47	The Effect of Sugarcane Straw Aging in the Field on Cell Wall Composition.
34335640	0	24	theme	Aging	30:34	arg1	Effect					4:9	The Effect	0:9	The Effect of Sugarcane Straw Aging in the Field on Cell Wall Composition.	0:73	The Effect of Sugarcane Straw Aging in the Field on Cell Wall Composition.
34335640	11	25	theme	wall	1444:1447	arg1	polysaccharides					1449:1463	the cell wall polysaccharides	1435:1463	the cell wall polysaccharides	1435:1463	The degradation and solubilization of the cell wall polysaccharides concomitantly increased the level of compounds related to recalcitrance, which led to a reduction in saccharification and an increase in minerals and ash contents.
34335640	3	26	theme	soil	480:483	arg1	quality					485:491	soil quality	480:491	soil quality	480:491	However, the process that leads to cell wall disassembly under field conditions is unknown, and understanding how this happens can improve sugarcane biorefinery and soil quality.
34335640	1	27	theme	production	152:161	arg1	amount					131:136	the amount	127:136	the amount of bioethanol production in the world	127:174	Cellulosic ethanol is an alternative for increasing the amount of bioethanol production in the world.
34335640	1	27	theme	production	152:161	arg1	production					152:161	bioethanol production	141:161	bioethanol production	141:161	Cellulosic ethanol is an alternative for increasing the amount of bioethanol production in the world.
34335640	0	28	from	Field	43:47	arg1	Effect					4:9	The Effect	0:9	The Effect of Sugarcane Straw Aging in the Field on Cell Wall Composition.	0:73	The Effect of Sugarcane Straw Aging in the Field on Cell Wall Composition.
34335640	6	29	theme	wall	769:772	arg1	fractionation					774:786	cell wall fractionation	764:786	cell wall fractionation	764:786	The cell wall composition was determined by cell wall fractionation and determination of monosaccharide composition.
34335640	11	30	theme	polysaccharides	1449:1463	arg1	solubilization					1417:1430	solubilization	1417:1430	solubilization	1417:1430	The degradation and solubilization of the cell wall polysaccharides concomitantly increased the level of compounds related to recalcitrance, which led to a reduction in saccharification and an increase in minerals and ash contents.
34335640	11	30	theme	polysaccharides	1449:1463	arg1	degradation					1401:1411	degradation	1401:1411	degradation	1401:1411	The degradation and solubilization of the cell wall polysaccharides concomitantly increased the level of compounds related to recalcitrance, which led to a reduction in saccharification and an increase in minerals and ash contents.
34335640	6	31	theme	monosaccharide	809:822	arg1	composition					824:834	monosaccharide composition	809:834	monosaccharide composition	809:834	The cell wall composition was determined by cell wall fractionation and determination of monosaccharide composition.
34335640	7	32	theme	Non-structural	837:850	arg1	carbohydrates					852:864	Non-structural carbohydrates	837:864	Non-structural carbohydrates	837:864	Non-structural carbohydrates degraded quickly during the first 3 months in the field.
34335640	8	33	theme	12.4	1048:1051	arg1	%					1052:1052	7.4 and 12.4%	1040:1052	%	1052:1052	Pectins and lignin remained in the plant waste for up to 12 months, while the hemicelluloses and cellulose decreased 7.4 and 12.4%, respectively.
34335640	10	34	theme	glucose	1316:1322	arg1	ratio					1324:1328	the xylose:glucose ratio	1305:1328	the xylose:glucose ratio	1305:1328	Despite cellulose reduction, the xylose:glucose ratio increased, suggesting that glucose is consumed faster than xylose.
34335640	0	35	theme	Sugarcane	14:22	arg1	Aging					30:34	Sugarcane Straw Aging	14:34	Sugarcane Straw Aging in the Field	14:47	The Effect of Sugarcane Straw Aging in the Field on Cell Wall Composition.
34335640	9	36	theme	arabinoxylan	1136:1147	arg1	solubilization					1118:1131	solubilization	1118:1131	solubilization of arabinoxylan (xylose and arabinose) and β-glucans (β-1,3 1,4 glucan; after 3 months) followed by degradation of cellulose (after 6 months)	1118:1273	Changes in monosaccharide compositions indicated solubilization of arabinoxylan (xylose and arabinose) and β-glucans (β-1,3 1,4 glucan; after 3 months) followed by degradation of cellulose (after 6 months).
34335640	1	37	theme	Cellulosic	75:84	arg1	alternative					100:110	an alternative	97:110	an alternative for increasing the amount of bioethanol production in the world	97:174	Cellulosic ethanol is an alternative for increasing the amount of bioethanol production in the world.
34335640	1	37	theme	Cellulosic	75:84	arg1	ethanol					86:92	Cellulosic ethanol	75:92	Cellulosic ethanol	75:92	Cellulosic ethanol is an alternative for increasing the amount of bioethanol production in the world.
34335640	7	38	theme	first	894:898	arg1	months					902:907	the first 3 months	890:907	the first 3 months in the field	890:920	Non-structural carbohydrates degraded quickly during the first 3 months in the field.
34335640	12	39	theme	material	1748:1755	arg1	mineralization					1726:1739	mineralization	1726:1739	mineralization of the material	1726:1755	Cellulose crystallinity changed little, with evidence of silica at the latter stages, indicating mineralization of the material.
34335640	6	40	theme	cell	764:767	arg1	fractionation					774:786	cell wall fractionation	764:786	cell wall fractionation	764:786	The cell wall composition was determined by cell wall fractionation and determination of monosaccharide composition.
34335640	7	41	from	months	902:907	arg1	field					916:920	the field	912:920	the field	912:920	Non-structural carbohydrates degraded quickly during the first 3 months in the field.
34335640	3	42	theme	cell	350:353	arg1	wall					355:358	cell wall	350:358	cell wall	350:358	However, the process that leads to cell wall disassembly under field conditions is unknown, and understanding how this happens can improve sugarcane biorefinery and soil quality.
34335640	4	43	theme	present	501:507	arg1	work					509:512	the present work	497:512	the present work	497:512	In the present work, we aimed at studying how sugarcane straw is degraded in the field after 3, 6, 9, and 12 months.
34335640	0	44	theme	Straw	24:28	arg1	Aging					30:34	Sugarcane Straw Aging	14:34	Sugarcane Straw Aging in the Field	14:47	The Effect of Sugarcane Straw Aging in the Field on Cell Wall Composition.
34335640	9	45	theme	cellulose	1248:1256	arg1	degradation					1233:1243	degradation	1233:1243	degradation of cellulose (after 6 months)	1233:1273	Changes in monosaccharide compositions indicated solubilization of arabinoxylan (xylose and arabinose) and β-glucans (β-1,3 1,4 glucan; after 3 months) followed by degradation of cellulose (after 6 months).
34335640	11	46	from	reduction	1553:1561	arg1	minerals					1602:1609	minerals	1602:1609	minerals	1602:1609	The degradation and solubilization of the cell wall polysaccharides concomitantly increased the level of compounds related to recalcitrance, which led to a reduction in saccharification and an increase in minerals and ash contents.
34335640	11	46	from	reduction	1553:1561	arg1	contents					1619:1626	ash contents	1615:1626	ash contents	1615:1626	The degradation and solubilization of the cell wall polysaccharides concomitantly increased the level of compounds related to recalcitrance, which led to a reduction in saccharification and an increase in minerals and ash contents.
34335640	11	46	from	reduction	1553:1561	arg1	saccharification					1566:1581	saccharification	1566:1581	saccharification	1566:1581	The degradation and solubilization of the cell wall polysaccharides concomitantly increased the level of compounds related to recalcitrance, which led to a reduction in saccharification and an increase in minerals and ash contents.
34335640	12	47	theme	latter	1700:1705	arg1	stages					1707:1712	the latter stages	1696:1712	the latter stages	1696:1712	Cellulose crystallinity changed little, with evidence of silica at the latter stages, indicating mineralization of the material.
34335640	11	48	from	increase	1590:1597	arg1	minerals					1602:1609	minerals	1602:1609	minerals	1602:1609	The degradation and solubilization of the cell wall polysaccharides concomitantly increased the level of compounds related to recalcitrance, which led to a reduction in saccharification and an increase in minerals and ash contents.
34335640	11	48	from	increase	1590:1597	arg1	contents					1619:1626	ash contents	1615:1626	ash contents	1615:1626	The degradation and solubilization of the cell wall polysaccharides concomitantly increased the level of compounds related to recalcitrance, which led to a reduction in saccharification and an increase in minerals and ash contents.
34335640	11	48	from	increase	1590:1597	arg1	saccharification					1566:1581	saccharification	1566:1581	saccharification	1566:1581	The degradation and solubilization of the cell wall polysaccharides concomitantly increased the level of compounds related to recalcitrance, which led to a reduction in saccharification and an increase in minerals and ash contents.
34335640	3	49	theme	field	378:382	arg1	conditions					384:393	field conditions	378:393	field conditions	378:393	However, the process that leads to cell wall disassembly under field conditions is unknown, and understanding how this happens can improve sugarcane biorefinery and soil quality.
34335640	5	50	theme	cellulose	681:689	arg1	crystallinity					691:703	cellulose crystallinity	681:703	cellulose crystallinity	681:703	Non-structural and structural carbohydrates, lignin content, ash, and cellulose crystallinity were analyzed.
34335640	14	51	used	used	1923:1926	arg2	straw					1907:1911	straw	1907:1911	straw	1907:1911	Alternatively, for bioenergy purposes, straw should be used in less than 3 months.
34335640	11	52	theme	ash	1615:1617	arg1	contents					1619:1626	ash contents	1615:1626	ash contents	1615:1626	The degradation and solubilization of the cell wall polysaccharides concomitantly increased the level of compounds related to recalcitrance, which led to a reduction in saccharification and an increase in minerals and ash contents.
34335640	9	53	theme	1,4	1193:1195	arg1	glucan					1197:1202	β-1,3 1,4 glucan	1187:1202	β-1,3 1,4 glucan; after 3 months	1187:1218	Changes in monosaccharide compositions indicated solubilization of arabinoxylan (xylose and arabinose) and β-glucans (β-1,3 1,4 glucan; after 3 months) followed by degradation of cellulose (after 6 months).
34335640	12	54	from	stages	1707:1712	arg1	evidence					1674:1681	evidence	1674:1681	evidence of silica at the latter stages	1674:1712	Cellulose crystallinity changed little, with evidence of silica at the latter stages, indicating mineralization of the material.
34335640	11	55	dep	degradation	1401:1411	arg1	The					1397:1399	The	1397:1399	The	1397:1399	The degradation and solubilization of the cell wall polysaccharides concomitantly increased the level of compounds related to recalcitrance, which led to a reduction in saccharification and an increase in minerals and ash contents.
34335640	10	56	theme	cellulose	1284:1292	arg1	reduction					1294:1302	cellulose reduction	1284:1302	cellulose reduction	1284:1302	Despite cellulose reduction, the xylose:glucose ratio increased, suggesting that glucose is consumed faster than xylose.
34335640	6	57	theme	wall	729:732	arg1	composition					734:744	The cell wall composition	720:744	The cell wall composition	720:744	The cell wall composition was determined by cell wall fractionation and determination of monosaccharide composition.
34335640	11	58	theme	cell	1439:1442	arg1	polysaccharides					1449:1463	the cell wall polysaccharides	1435:1463	the cell wall polysaccharides	1435:1463	The degradation and solubilization of the cell wall polysaccharides concomitantly increased the level of compounds related to recalcitrance, which led to a reduction in saccharification and an increase in minerals and ash contents.
34335640	6	59	theme	cell	724:727	arg1	composition					734:744	The cell wall composition	720:744	The cell wall composition	720:744	The cell wall composition was determined by cell wall fractionation and determination of monosaccharide composition.
34335640	11	60	theme	related	1512:1518	arg1	compounds					1502:1510	compounds	1502:1510	compounds related to recalcitrance, which led to a reduction in saccharification and an increase in minerals and ash contents	1502:1626	The degradation and solubilization of the cell wall polysaccharides concomitantly increased the level of compounds related to recalcitrance, which led to a reduction in saccharification and an increase in minerals and ash contents.
34335640	2	61	theme	sugarcane	274:282	arg1	feedstock					304:312	a possible feedstock	293:312	a possible feedstock	293:312	In Brazil, sugarcane leads the bioethanol production, and to improve its yield, besides bagasse, sugarcane straw is a possible feedstock.
34335640	2	61	theme	sugarcane	274:282	arg1	straw					284:288	sugarcane straw	274:288	sugarcane straw	274:288	In Brazil, sugarcane leads the bioethanol production, and to improve its yield, besides bagasse, sugarcane straw is a possible feedstock.
34335640	8	62	theme	7.4	1040:1042	arg1	%					1052:1052	7.4 and 12.4%	1040:1052	%	1052:1052	Pectins and lignin remained in the plant waste for up to 12 months, while the hemicelluloses and cellulose decreased 7.4 and 12.4%, respectively.
34335640	13	63	theme	better	1784:1789	arg1	mineralization					1796:1809	better soil mineralization	1784:1809	better soil mineralization	1784:1809	Our data suggest that for better soil mineralization, sugarcane straw must stay in the field for over 1 year.
34335640	3	64	theme	sugarcane	454:462	arg1	biorefinery					464:474	sugarcane biorefinery	454:474	sugarcane biorefinery	454:474	However, the process that leads to cell wall disassembly under field conditions is unknown, and understanding how this happens can improve sugarcane biorefinery and soil quality.
34335640	13	65	theme	sugarcane	1812:1820	arg1	straw					1822:1826	sugarcane straw	1812:1826	sugarcane straw	1812:1826	Our data suggest that for better soil mineralization, sugarcane straw must stay in the field for over 1 year.
34335640	5	66	theme	lignin	656:661	arg1	content					663:669	lignin content	656:669	lignin content	656:669	Non-structural and structural carbohydrates, lignin content, ash, and cellulose crystallinity were analyzed.
34335640	9	67	theme	β-1,3	1187:1191	arg1	glucan					1197:1202	β-1,3 1,4 glucan	1187:1202	β-1,3 1,4 glucan; after 3 months	1187:1218	Changes in monosaccharide compositions indicated solubilization of arabinoxylan (xylose and arabinose) and β-glucans (β-1,3 1,4 glucan; after 3 months) followed by degradation of cellulose (after 6 months).
34335640	2	68	theme	bioethanol	208:217	arg1	production					219:228	the bioethanol production	204:228	the bioethanol production	204:228	In Brazil, sugarcane leads the bioethanol production, and to improve its yield, besides bagasse, sugarcane straw is a possible feedstock.
34335640	4	69	theme	sugarcane	540:548	arg1	straw					550:554	sugarcane straw	540:554	sugarcane straw	540:554	In the present work, we aimed at studying how sugarcane straw is degraded in the field after 3, 6, 9, and 12 months.
33565638	4	0	theme	OFSP-based	505:514	arg1	foods					527:531	OFSP-based functional foods	505:531	OFSP-based functional foods	505:531	In view of developing OFSP-based functional foods to address the vitamin A deficiency (VAD) issues, herein, OFSP puree-wheat composite breads have been prepared at 10% to 50% OFSP puree concentrations and bioaccessibility of βC has been estimated.
33565638	9	1	theme	Starch	1358:1363	arg1	amount					1371:1376	the Rapidly Digestible Starch (RDS) amount	1335:1376	the Rapidly Digestible Starch (RDS) amount coupled with increase in the Slowly Digestible Starch (SDS) and Resistant Starch (RS) fractions	1335:1472	Furthermore, in vitro starch digestion decreases with significant reduction in the Rapidly Digestible Starch (RDS) amount coupled with increase in the Slowly Digestible Starch (SDS) and Resistant Starch (RS) fractions.
33565638	3	2	theme	provitamin	458:467	arg1	carotenoid					471:480	a provitamin A carotenoid	456:480	a provitamin A carotenoid	456:480	Foods enriched with naturally occurring carotenes have the potential, in this regard, and orange-fleshed sweet potato (OFSP) stands out tall as it is rich in β-carotene (βC), a provitamin A carotenoid.
33565638	3	2	theme	provitamin	458:467	arg1	β-carotene					439:448	β-carotene (βC)	439:453	β-carotene (βC)	439:453	Foods enriched with naturally occurring carotenes have the potential, in this regard, and orange-fleshed sweet potato (OFSP) stands out tall as it is rich in β-carotene (βC), a provitamin A carotenoid.
33565638	9	3	dep	in	1269:1270	arg1	vitro					1272:1276	vitro	1272:1276	vitro	1272:1276	Furthermore, in vitro starch digestion decreases with significant reduction in the Rapidly Digestible Starch (RDS) amount coupled with increase in the Slowly Digestible Starch (SDS) and Resistant Starch (RS) fractions.
33565638	4	4	dep	concentrations	669:682	arg1	%					656:656	%	656:656	%	656:656	In view of developing OFSP-based functional foods to address the vitamin A deficiency (VAD) issues, herein, OFSP puree-wheat composite breads have been prepared at 10% to 50% OFSP puree concentrations and bioaccessibility of βC has been estimated.
33565638	4	5	theme	deficiency	558:567	arg1	issues					575:580	the vitamin A deficiency (VAD) issues	544:580	the vitamin A deficiency (VAD) issues	544:580	In view of developing OFSP-based functional foods to address the vitamin A deficiency (VAD) issues, herein, OFSP puree-wheat composite breads have been prepared at 10% to 50% OFSP puree concentrations and bioaccessibility of βC has been estimated.
33565638	11	6	theme	functional	1692:1701	arg1	foods					1703:1707	novel functional foods	1686:1707	novel functional foods that could address the VAD as well as glycemic issues toward improving human health	1686:1791	The amount of bioaccessible βC coupled with altered starch digestion of the OFSP wheat breads highlight their usefulness as novel functional foods that could address the VAD as well as glycemic issues toward improving human health.
33565638	11	7	theme	starch	1614:1619	arg1	digestion					1621:1629	altered starch digestion	1606:1629	altered starch digestion of the OFSP wheat breads	1606:1654	The amount of bioaccessible βC coupled with altered starch digestion of the OFSP wheat breads highlight their usefulness as novel functional foods that could address the VAD as well as glycemic issues toward improving human health.
33565638	4	8	dep	%	649:649	arg1	to					651:652	to	651:652	to	651:652	In view of developing OFSP-based functional foods to address the vitamin A deficiency (VAD) issues, herein, OFSP puree-wheat composite breads have been prepared at 10% to 50% OFSP puree concentrations and bioaccessibility of βC has been estimated.
33565638	11	9	theme	wheat	1643:1647	arg1	breads					1649:1654	the OFSP wheat breads	1634:1654	the OFSP wheat breads	1634:1654	The amount of bioaccessible βC coupled with altered starch digestion of the OFSP wheat breads highlight their usefulness as novel functional foods that could address the VAD as well as glycemic issues toward improving human health.
33565638	10	10	theme	OFSP-wheat	1484:1493	arg1	bread					1505:1509	OFSP-wheat composite bread	1484:1509	OFSP-wheat composite bread	1484:1509	Overall, OFSP-wheat composite bread holds adequate amount of provitamin A carotenoids.
33565638	9	11	theme	Starch	1425:1430	arg1	fractions					1464:1472	the Slowly Digestible Starch (SDS) and Resistant Starch (RS) fractions	1403:1472	the Slowly Digestible Starch (SDS) and Resistant Starch (RS) fractions	1403:1472	Furthermore, in vitro starch digestion decreases with significant reduction in the Rapidly Digestible Starch (RDS) amount coupled with increase in the Slowly Digestible Starch (SDS) and Resistant Starch (RS) fractions.
33565638	4	12	theme	OFSP	591:594	arg1	breads					618:623	OFSP puree-wheat composite breads	591:623	OFSP puree-wheat composite breads	591:623	In view of developing OFSP-based functional foods to address the vitamin A deficiency (VAD) issues, herein, OFSP puree-wheat composite breads have been prepared at 10% to 50% OFSP puree concentrations and bioaccessibility of βC has been estimated.
33565638	7	13	theme	all-trans-βC	1000:1011	arg1	micellarization					981:995	micellarization	981:995	micellarization of all-trans-βC, 13-cis βC, and 9-cis βC after simulated oral, gastric, and small intestinal digestion	981:1098	The efficiency of micellarization of all-trans-βC, 13-cis βC, and 9-cis βC after simulated oral, gastric, and small intestinal digestion are 1.4% to 6.4%, 1.4% to 7.2%, and 1.1% to 6.9%, respectively.
33565638	11	14	theme	OFSP	1638:1641	arg1	breads					1649:1654	the OFSP wheat breads	1634:1654	the OFSP wheat breads	1634:1654	The amount of bioaccessible βC coupled with altered starch digestion of the OFSP wheat breads highlight their usefulness as novel functional foods that could address the VAD as well as glycemic issues toward improving human health.
33565638	1	15	theme	human	143:147	arg1	health					149:154	human health	143:154	human health	143:154	Vitamin A is essential for vision, human health, growth, immune function, and reproduction.
33565638	4	16	theme	composite	608:616	arg1	breads					618:623	OFSP puree-wheat composite breads	591:623	OFSP puree-wheat composite breads	591:623	In view of developing OFSP-based functional foods to address the vitamin A deficiency (VAD) issues, herein, OFSP puree-wheat composite breads have been prepared at 10% to 50% OFSP puree concentrations and bioaccessibility of βC has been estimated.
33565638	0	17	theme	potato	82:87	arg1	bread					101:105	the orange- fleshed sweet potato puree-wheat bread	56:105	the orange- fleshed sweet potato puree-wheat bread	56:105	Starch digestibility and β-carotene bioaccessibility in the orange- fleshed sweet potato puree-wheat bread.
33565638	9	18	from	increase	1391:1398	arg1	fractions					1464:1472	the Slowly Digestible Starch (SDS) and Resistant Starch (RS) fractions	1403:1472	the Slowly Digestible Starch (SDS) and Resistant Starch (RS) fractions	1403:1472	Furthermore, in vitro starch digestion decreases with significant reduction in the Rapidly Digestible Starch (RDS) amount coupled with increase in the Slowly Digestible Starch (SDS) and Resistant Starch (RS) fractions.
33565638	8	19	theme	micellarized	1178:1189	arg1	βC					1191:1192	micellarized βC	1178:1192	micellarized βC	1178:1192	The amount of micellarized βC correlates linearly with the OFSP concentration in the bread.
33565638	9	20	theme	Starch	1452:1457	arg1	fractions					1464:1472	the Slowly Digestible Starch (SDS) and Resistant Starch (RS) fractions	1403:1472	the Slowly Digestible Starch (SDS) and Resistant Starch (RS) fractions	1403:1472	Furthermore, in vitro starch digestion decreases with significant reduction in the Rapidly Digestible Starch (RDS) amount coupled with increase in the Slowly Digestible Starch (SDS) and Resistant Starch (RS) fractions.
33565638	4	21	theme	puree	663:667	arg1	concentrations					669:682	10% to 50% OFSP puree concentrations	647:682	10% to 50% OFSP puree concentrations	647:682	In view of developing OFSP-based functional foods to address the vitamin A deficiency (VAD) issues, herein, OFSP puree-wheat composite breads have been prepared at 10% to 50% OFSP puree concentrations and bioaccessibility of βC has been estimated.
33565638	6	22	theme	retinol	877:883	arg1	RAE					907:909	RAE	907:909	RAE	907:909	The corresponding calculated retinol activity equivalents (RAE) are 30.9, 66.4, 119.5, 170.4, and 246.2 RAE/100 g.
33565638	6	22	theme	retinol	877:883	arg1	equivalents					894:904	The corresponding calculated retinol activity equivalents	848:904	The corresponding calculated retinol activity equivalents (RAE)	848:910	The corresponding calculated retinol activity equivalents (RAE) are 30.9, 66.4, 119.5, 170.4, and 246.2 RAE/100 g.
33565638	6	22	theme	retinol	877:883	arg1	g					960:960	30.9, 66.4, 119.5, 170.4, and 246.2 RAE/100 g	916:960	30.9, 66.4, 119.5, 170.4, and 246.2 RAE/100 g	916:960	The corresponding calculated retinol activity equivalents (RAE) are 30.9, 66.4, 119.5, 170.4, and 246.2 RAE/100 g.
33565638	0	23	from	bioaccessibility	36:51	arg1	bread					101:105	the orange- fleshed sweet potato puree-wheat bread	56:105	the orange- fleshed sweet potato puree-wheat bread	56:105	Starch digestibility and β-carotene bioaccessibility in the orange- fleshed sweet potato puree-wheat bread.
33565638	3	24	theme	occurring	311:319	arg1	carotenes					321:329	naturally occurring carotenes	301:329	naturally occurring carotenes	301:329	Foods enriched with naturally occurring carotenes have the potential, in this regard, and orange-fleshed sweet potato (OFSP) stands out tall as it is rich in β-carotene (βC), a provitamin A carotenoid.
33565638	4	25	theme	10	647:648	arg1	%					649:649	%	649:649	%	649:649	In view of developing OFSP-based functional foods to address the vitamin A deficiency (VAD) issues, herein, OFSP puree-wheat composite breads have been prepared at 10% to 50% OFSP puree concentrations and bioaccessibility of βC has been estimated.
33565638	0	26	theme	Starch	0:5	arg1	digestibility					7:19	Starch digestibility	0:19	Starch digestibility	0:19	Starch digestibility and β-carotene bioaccessibility in the orange- fleshed sweet potato puree-wheat bread.
33565638	6	27	theme	corresponding	852:864	arg1	RAE					907:909	RAE	907:909	RAE	907:909	The corresponding calculated retinol activity equivalents (RAE) are 30.9, 66.4, 119.5, 170.4, and 246.2 RAE/100 g.
33565638	6	27	theme	corresponding	852:864	arg1	equivalents					894:904	The corresponding calculated retinol activity equivalents	848:904	The corresponding calculated retinol activity equivalents (RAE)	848:910	The corresponding calculated retinol activity equivalents (RAE) are 30.9, 66.4, 119.5, 170.4, and 246.2 RAE/100 g.
33565638	6	27	theme	corresponding	852:864	arg1	g					960:960	30.9, 66.4, 119.5, 170.4, and 246.2 RAE/100 g	916:960	30.9, 66.4, 119.5, 170.4, and 246.2 RAE/100 g	916:960	The corresponding calculated retinol activity equivalents (RAE) are 30.9, 66.4, 119.5, 170.4, and 246.2 RAE/100 g.
33565638	8	28	theme	OFSP	1223:1226	arg1	concentration					1228:1240	the OFSP concentration	1219:1240	the OFSP concentration in the bread	1219:1253	The amount of micellarized βC correlates linearly with the OFSP concentration in the bread.
33565638	7	29	theme	gastric	1060:1066	arg1	digestion					1090:1098	simulated oral, gastric, and small intestinal digestion	1044:1098	simulated oral, gastric, and small intestinal digestion	1044:1098	The efficiency of micellarization of all-trans-βC, 13-cis βC, and 9-cis βC after simulated oral, gastric, and small intestinal digestion are 1.4% to 6.4%, 1.4% to 7.2%, and 1.1% to 6.9%, respectively.
33565638	3	30	theme	sweet	386:390	arg1	OFSP					400:403	OFSP	400:403	OFSP	400:403	Foods enriched with naturally occurring carotenes have the potential, in this regard, and orange-fleshed sweet potato (OFSP) stands out tall as it is rich in β-carotene (βC), a provitamin A carotenoid.
33565638	3	30	theme	sweet	386:390	arg1	potato					392:397	orange-fleshed sweet potato	371:397	orange-fleshed sweet potato (OFSP)	371:404	Foods enriched with naturally occurring carotenes have the potential, in this regard, and orange-fleshed sweet potato (OFSP) stands out tall as it is rich in β-carotene (βC), a provitamin A carotenoid.
33565638	10	31	theme	adequate	1517:1524	arg1	amount					1526:1531	adequate amount	1517:1531	adequate amount of provitamin A carotenoids	1517:1559	Overall, OFSP-wheat composite bread holds adequate amount of provitamin A carotenoids.
33565638	10	31	theme	adequate	1517:1524	arg1	carotenoids					1549:1559	provitamin A carotenoids	1536:1559	provitamin A carotenoids	1536:1559	Overall, OFSP-wheat composite bread holds adequate amount of provitamin A carotenoids.
33565638	11	32	theme	bioaccessible	1576:1588	arg1	βC					1590:1591	bioaccessible βC	1576:1591	bioaccessible βC coupled with altered starch digestion of the OFSP wheat breads	1576:1654	The amount of bioaccessible βC coupled with altered starch digestion of the OFSP wheat breads highlight their usefulness as novel functional foods that could address the VAD as well as glycemic issues toward improving human health.
33565638	7	33	theme	oral	1054:1057	arg1	digestion					1090:1098	simulated oral, gastric, and small intestinal digestion	1044:1098	simulated oral, gastric, and small intestinal digestion	1044:1098	The efficiency of micellarization of all-trans-βC, 13-cis βC, and 9-cis βC after simulated oral, gastric, and small intestinal digestion are 1.4% to 6.4%, 1.4% to 7.2%, and 1.1% to 6.9%, respectively.
33565638	0	34	theme	fleshed	68:74	arg1	bread					101:105	the orange- fleshed sweet potato puree-wheat bread	56:105	the orange- fleshed sweet potato puree-wheat bread	56:105	Starch digestibility and β-carotene bioaccessibility in the orange- fleshed sweet potato puree-wheat bread.
33565638	10	35	theme	A	1547:1547	arg1	carotenoids					1549:1559	provitamin A carotenoids	1536:1559	provitamin A carotenoids	1536:1559	Overall, OFSP-wheat composite bread holds adequate amount of provitamin A carotenoids.
33565638	7	36	theme	9-cis	1029:1033	arg1	βC					1035:1036	9-cis βC	1029:1036	9-cis βC	1029:1036	The efficiency of micellarization of all-trans-βC, 13-cis βC, and 9-cis βC after simulated oral, gastric, and small intestinal digestion are 1.4% to 6.4%, 1.4% to 7.2%, and 1.1% to 6.9%, respectively.
33565638	11	37	theme	human	1780:1784	arg1	health					1786:1791	human health	1780:1791	human health	1780:1791	The amount of bioaccessible βC coupled with altered starch digestion of the OFSP wheat breads highlight their usefulness as novel functional foods that could address the VAD as well as glycemic issues toward improving human health.
33565638	7	38	theme	13-cis	1014:1019	arg1	βC					1021:1022	13-cis βC	1014:1022	13-cis βC	1014:1022	The efficiency of micellarization of all-trans-βC, 13-cis βC, and 9-cis βC after simulated oral, gastric, and small intestinal digestion are 1.4% to 6.4%, 1.4% to 7.2%, and 1.1% to 6.9%, respectively.
33565638	8	39	from	concentration	1228:1240	arg1	bread					1249:1253	the bread	1245:1253	the bread	1245:1253	The amount of micellarized βC correlates linearly with the OFSP concentration in the bread.
33565638	5	40	theme	OFSP	822:825	arg1	βC					741:742	The total βC	731:742	The total βC	731:742	The total βC is found to be 4.3, 9.2, 16.5, 23.3, and 33.6 µg/g in 10, 20, 30, 40, and 50% OFSP bread, respectively.
33565638	5	40	theme	OFSP	822:825	arg1	bread					827:831	4.3, 9.2, 16.5, 23.3, and 33.6 µg/g in 10, 20, 30, 40, and 50% OFSP bread	759:831	4.3, 9.2, 16.5, 23.3, and 33.6 µg/g in 10, 20, 30, 40, and 50% OFSP bread	759:831	The total βC is found to be 4.3, 9.2, 16.5, 23.3, and 33.6 µg/g in 10, 20, 30, 40, and 50% OFSP bread, respectively.
33565638	3	41	theme	A	469:469	arg1	carotenoid					471:480	a provitamin A carotenoid	456:480	a provitamin A carotenoid	456:480	Foods enriched with naturally occurring carotenes have the potential, in this regard, and orange-fleshed sweet potato (OFSP) stands out tall as it is rich in β-carotene (βC), a provitamin A carotenoid.
33565638	3	41	theme	A	469:469	arg1	β-carotene					439:448	β-carotene (βC)	439:453	β-carotene (βC)	439:453	Foods enriched with naturally occurring carotenes have the potential, in this regard, and orange-fleshed sweet potato (OFSP) stands out tall as it is rich in β-carotene (βC), a provitamin A carotenoid.
33565638	9	42	theme	Digestible	1347:1356	arg1	RDS					1366:1368	RDS	1366:1368	RDS	1366:1368	Furthermore, in vitro starch digestion decreases with significant reduction in the Rapidly Digestible Starch (RDS) amount coupled with increase in the Slowly Digestible Starch (SDS) and Resistant Starch (RS) fractions.
33565638	9	42	theme	Digestible	1347:1356	arg1	Starch					1358:1363	the Rapidly Digestible Starch	1335:1363	the Rapidly Digestible Starch (RDS) amount coupled with increase in the Slowly Digestible Starch (SDS) and Resistant Starch (RS) fractions	1335:1472	Furthermore, in vitro starch digestion decreases with significant reduction in the Rapidly Digestible Starch (RDS) amount coupled with increase in the Slowly Digestible Starch (SDS) and Resistant Starch (RS) fractions.
33565638	4	43	theme	functional	516:525	arg1	foods					527:531	OFSP-based functional foods	505:531	OFSP-based functional foods	505:531	In view of developing OFSP-based functional foods to address the vitamin A deficiency (VAD) issues, herein, OFSP puree-wheat composite breads have been prepared at 10% to 50% OFSP puree concentrations and bioaccessibility of βC has been estimated.
33565638	2	44	theme	growth	251:256	arg1	reduction					258:266	growth reduction	251:266	growth reduction	251:266	Its deficiency leads to anemia, xerophthalmia, and growth reduction in children.
33565638	1	45	theme	immune	165:170	arg1	function					172:179	immune function	165:179	immune function	165:179	Vitamin A is essential for vision, human health, growth, immune function, and reproduction.
33565638	7	46	theme	%	1139:1139	arg1	%					1147:1147	1.1% to 6.9%	1136:1147	1.1% to 6.9%	1136:1147	The efficiency of micellarization of all-trans-βC, 13-cis βC, and 9-cis βC after simulated oral, gastric, and small intestinal digestion are 1.4% to 6.4%, 1.4% to 7.2%, and 1.1% to 6.9%, respectively.
33565638	5	47	dep	bread	827:831	arg1	%					820:820	%	820:820	%	820:820	The total βC is found to be 4.3, 9.2, 16.5, 23.3, and 33.6 µg/g in 10, 20, 30, 40, and 50% OFSP bread, respectively.
33565638	7	48	theme	micellarization	981:995	arg1	efficiency					967:976	The efficiency	963:976	The efficiency of micellarization of all-trans-βC, 13-cis βC, and 9-cis βC after simulated oral, gastric, and small intestinal digestion	963:1098	The efficiency of micellarization of all-trans-βC, 13-cis βC, and 9-cis βC after simulated oral, gastric, and small intestinal digestion are 1.4% to 6.4%, 1.4% to 7.2%, and 1.1% to 6.9%, respectively.
33565638	7	48	theme	micellarization	981:995	arg1	%					1115:1115	1.4% to 6.4%	1104:1115	1.4% to 6.4%	1104:1115	The efficiency of micellarization of all-trans-βC, 13-cis βC, and 9-cis βC after simulated oral, gastric, and small intestinal digestion are 1.4% to 6.4%, 1.4% to 7.2%, and 1.1% to 6.9%, respectively.
33565638	3	49	from	rich	431:434	arg1	carotenoid					471:480	a provitamin A carotenoid	456:480	a provitamin A carotenoid	456:480	Foods enriched with naturally occurring carotenes have the potential, in this regard, and orange-fleshed sweet potato (OFSP) stands out tall as it is rich in β-carotene (βC), a provitamin A carotenoid.
33565638	3	49	from	rich	431:434	arg1	β-carotene					439:448	β-carotene (βC)	439:453	β-carotene (βC)	439:453	Foods enriched with naturally occurring carotenes have the potential, in this regard, and orange-fleshed sweet potato (OFSP) stands out tall as it is rich in β-carotene (βC), a provitamin A carotenoid.
33565638	7	50	dep	%	1115:1115	arg1	to					1109:1110	to	1109:1110	to	1109:1110	The efficiency of micellarization of all-trans-βC, 13-cis βC, and 9-cis βC after simulated oral, gastric, and small intestinal digestion are 1.4% to 6.4%, 1.4% to 7.2%, and 1.1% to 6.9%, respectively.
33565638	8	51	theme	βC	1191:1192	arg1	amount					1168:1173	The amount	1164:1173	The amount of micellarized βC	1164:1192	The amount of micellarized βC correlates linearly with the OFSP concentration in the bread.
33565638	8	51	theme	βC	1191:1192	arg1	βC					1191:1192	micellarized βC	1178:1192	micellarized βC	1178:1192	The amount of micellarized βC correlates linearly with the OFSP concentration in the bread.
33565638	5	52	dep	%	820:820	arg1	10					798:799	10	798:799	10	798:799	The total βC is found to be 4.3, 9.2, 16.5, 23.3, and 33.6 µg/g in 10, 20, 30, 40, and 50% OFSP bread, respectively.
33565638	5	52	dep	%	820:820	arg1	33.6 µg/g					785:793	33.6 µg/g	785:793	33.6 µg/g	785:793	The total βC is found to be 4.3, 9.2, 16.5, 23.3, and 33.6 µg/g in 10, 20, 30, 40, and 50% OFSP bread, respectively.
33565638	5	52	dep	%	820:820	arg1	50					818:819	50	818:819	50	818:819	The total βC is found to be 4.3, 9.2, 16.5, 23.3, and 33.6 µg/g in 10, 20, 30, 40, and 50% OFSP bread, respectively.
33565638	3	53	from	β-carotene	439:448	arg1	rich					431:434	rich	431:434	rich	431:434	Foods enriched with naturally occurring carotenes have the potential, in this regard, and orange-fleshed sweet potato (OFSP) stands out tall as it is rich in β-carotene (βC), a provitamin A carotenoid.
33565638	11	54	theme	novel	1686:1690	arg1	foods					1703:1707	novel functional foods	1686:1707	novel functional foods that could address the VAD as well as glycemic issues toward improving human health	1686:1791	The amount of bioaccessible βC coupled with altered starch digestion of the OFSP wheat breads highlight their usefulness as novel functional foods that could address the VAD as well as glycemic issues toward improving human health.
33565638	11	55	theme	VAD	1732:1734	arg1	issues					1756:1761	the VAD as well as glycemic issues	1728:1761	the VAD as well as glycemic issues toward improving human health	1728:1791	The amount of bioaccessible βC coupled with altered starch digestion of the OFSP wheat breads highlight their usefulness as novel functional foods that could address the VAD as well as glycemic issues toward improving human health.
33565638	7	56	theme	%	1121:1121	arg1	%					1129:1129	1.4% to 7.2%	1118:1129	1.4% to 7.2%	1118:1129	The efficiency of micellarization of all-trans-βC, 13-cis βC, and 9-cis βC after simulated oral, gastric, and small intestinal digestion are 1.4% to 6.4%, 1.4% to 7.2%, and 1.1% to 6.9%, respectively.
33565638	4	57	theme	A	556:556	arg1	VAD					570:572	VAD	570:572	VAD	570:572	In view of developing OFSP-based functional foods to address the vitamin A deficiency (VAD) issues, herein, OFSP puree-wheat composite breads have been prepared at 10% to 50% OFSP puree concentrations and bioaccessibility of βC has been estimated.
33565638	4	57	theme	A	556:556	arg1	deficiency					558:567	the vitamin A deficiency	544:567	the vitamin A deficiency (VAD) issues	544:580	In view of developing OFSP-based functional foods to address the vitamin A deficiency (VAD) issues, herein, OFSP puree-wheat composite breads have been prepared at 10% to 50% OFSP puree concentrations and bioaccessibility of βC has been estimated.
33565638	5	58	theme	total	735:739	arg1	βC					741:742	The total βC	731:742	The total βC	731:742	The total βC is found to be 4.3, 9.2, 16.5, 23.3, and 33.6 µg/g in 10, 20, 30, 40, and 50% OFSP bread, respectively.
33565638	5	58	theme	total	735:739	arg1	bread					827:831	4.3, 9.2, 16.5, 23.3, and 33.6 µg/g in 10, 20, 30, 40, and 50% OFSP bread	759:831	4.3, 9.2, 16.5, 23.3, and 33.6 µg/g in 10, 20, 30, 40, and 50% OFSP bread	759:831	The total βC is found to be 4.3, 9.2, 16.5, 23.3, and 33.6 µg/g in 10, 20, 30, 40, and 50% OFSP bread, respectively.
33565638	11	59	theme	altered	1606:1612	arg1	digestion					1621:1629	altered starch digestion	1606:1629	altered starch digestion of the OFSP wheat breads	1606:1654	The amount of bioaccessible βC coupled with altered starch digestion of the OFSP wheat breads highlight their usefulness as novel functional foods that could address the VAD as well as glycemic issues toward improving human health.
33565638	7	60	dep	%	1129:1129	arg1	to					1123:1124	to	1123:1124	to	1123:1124	The efficiency of micellarization of all-trans-βC, 13-cis βC, and 9-cis βC after simulated oral, gastric, and small intestinal digestion are 1.4% to 6.4%, 1.4% to 7.2%, and 1.1% to 6.9%, respectively.
33565638	11	61	theme	glycemic	1747:1754	arg1	issues					1756:1761	the VAD as well as glycemic issues	1728:1761	the VAD as well as glycemic issues toward improving human health	1728:1791	The amount of bioaccessible βC coupled with altered starch digestion of the OFSP wheat breads highlight their usefulness as novel functional foods that could address the VAD as well as glycemic issues toward improving human health.
33565638	10	62	theme	composite	1495:1503	arg1	bread					1505:1509	OFSP-wheat composite bread	1484:1509	OFSP-wheat composite bread	1484:1509	Overall, OFSP-wheat composite bread holds adequate amount of provitamin A carotenoids.
33565638	9	63	theme	Digestible	1414:1423	arg1	SDS					1433:1435	SDS	1433:1435	SDS	1433:1435	Furthermore, in vitro starch digestion decreases with significant reduction in the Rapidly Digestible Starch (RDS) amount coupled with increase in the Slowly Digestible Starch (SDS) and Resistant Starch (RS) fractions.
33565638	9	63	theme	Digestible	1414:1423	arg1	Starch					1425:1430	the Slowly Digestible Starch	1403:1430	the Slowly Digestible Starch (SDS)	1403:1436	Furthermore, in vitro starch digestion decreases with significant reduction in the Rapidly Digestible Starch (RDS) amount coupled with increase in the Slowly Digestible Starch (SDS) and Resistant Starch (RS) fractions.
33565638	9	63	theme	Digestible	1414:1423	arg1	RS					1460:1461	RS	1460:1461	RS	1460:1461	Furthermore, in vitro starch digestion decreases with significant reduction in the Rapidly Digestible Starch (RDS) amount coupled with increase in the Slowly Digestible Starch (SDS) and Resistant Starch (RS) fractions.
33565638	7	64	theme	%	1107:1107	arg1	efficiency					967:976	The efficiency	963:976	The efficiency of micellarization of all-trans-βC, 13-cis βC, and 9-cis βC after simulated oral, gastric, and small intestinal digestion	963:1098	The efficiency of micellarization of all-trans-βC, 13-cis βC, and 9-cis βC after simulated oral, gastric, and small intestinal digestion are 1.4% to 6.4%, 1.4% to 7.2%, and 1.1% to 6.9%, respectively.
33565638	7	64	theme	%	1107:1107	arg1	%					1115:1115	1.4% to 6.4%	1104:1115	1.4% to 6.4%	1104:1115	The efficiency of micellarization of all-trans-βC, 13-cis βC, and 9-cis βC after simulated oral, gastric, and small intestinal digestion are 1.4% to 6.4%, 1.4% to 7.2%, and 1.1% to 6.9%, respectively.
33565638	0	65	theme	sweet	76:80	arg1	bread					101:105	the orange- fleshed sweet potato puree-wheat bread	56:105	the orange- fleshed sweet potato puree-wheat bread	56:105	Starch digestibility and β-carotene bioaccessibility in the orange- fleshed sweet potato puree-wheat bread.
33565638	2	66	from	reduction	258:266	arg1	children					271:278	children	271:278	children	271:278	Its deficiency leads to anemia, xerophthalmia, and growth reduction in children.
33565638	0	67	theme	puree-wheat	89:99	arg1	bread					101:105	the orange- fleshed sweet potato puree-wheat bread	56:105	the orange- fleshed sweet potato puree-wheat bread	56:105	Starch digestibility and β-carotene bioaccessibility in the orange- fleshed sweet potato puree-wheat bread.
33565638	4	68	dep	%	656:656	arg1	%					649:649	%	649:649	%	649:649	In view of developing OFSP-based functional foods to address the vitamin A deficiency (VAD) issues, herein, OFSP puree-wheat composite breads have been prepared at 10% to 50% OFSP puree concentrations and bioaccessibility of βC has been estimated.
33565638	0	69	from	digestibility	7:19	arg1	bread					101:105	the orange- fleshed sweet potato puree-wheat bread	56:105	the orange- fleshed sweet potato puree-wheat bread	56:105	Starch digestibility and β-carotene bioaccessibility in the orange- fleshed sweet potato puree-wheat bread.
33565638	4	70	theme	puree-wheat	596:606	arg1	breads					618:623	OFSP puree-wheat composite breads	591:623	OFSP puree-wheat composite breads	591:623	In view of developing OFSP-based functional foods to address the vitamin A deficiency (VAD) issues, herein, OFSP puree-wheat composite breads have been prepared at 10% to 50% OFSP puree concentrations and bioaccessibility of βC has been estimated.
33565638	9	71	theme	Resistant	1442:1450	arg1	Starch					1452:1457	Resistant Starch	1442:1457	Resistant Starch	1442:1457	Furthermore, in vitro starch digestion decreases with significant reduction in the Rapidly Digestible Starch (RDS) amount coupled with increase in the Slowly Digestible Starch (SDS) and Resistant Starch (RS) fractions.
33565638	4	72	theme	50	654:655	arg1	%					649:649	%	649:649	%	649:649	In view of developing OFSP-based functional foods to address the vitamin A deficiency (VAD) issues, herein, OFSP puree-wheat composite breads have been prepared at 10% to 50% OFSP puree concentrations and bioaccessibility of βC has been estimated.
33565638	9	73	from	reduction	1322:1330	arg1	amount					1371:1376	the Rapidly Digestible Starch (RDS) amount	1335:1376	the Rapidly Digestible Starch (RDS) amount coupled with increase in the Slowly Digestible Starch (SDS) and Resistant Starch (RS) fractions	1335:1472	Furthermore, in vitro starch digestion decreases with significant reduction in the Rapidly Digestible Starch (RDS) amount coupled with increase in the Slowly Digestible Starch (SDS) and Resistant Starch (RS) fractions.
33565638	6	74	theme	activity	885:892	arg1	RAE					907:909	RAE	907:909	RAE	907:909	The corresponding calculated retinol activity equivalents (RAE) are 30.9, 66.4, 119.5, 170.4, and 246.2 RAE/100 g.
33565638	6	74	theme	activity	885:892	arg1	equivalents					894:904	The corresponding calculated retinol activity equivalents	848:904	The corresponding calculated retinol activity equivalents (RAE)	848:910	The corresponding calculated retinol activity equivalents (RAE) are 30.9, 66.4, 119.5, 170.4, and 246.2 RAE/100 g.
33565638	6	74	theme	activity	885:892	arg1	g					960:960	30.9, 66.4, 119.5, 170.4, and 246.2 RAE/100 g	916:960	30.9, 66.4, 119.5, 170.4, and 246.2 RAE/100 g	916:960	The corresponding calculated retinol activity equivalents (RAE) are 30.9, 66.4, 119.5, 170.4, and 246.2 RAE/100 g.
33565638	4	75	theme	OFSP	658:661	arg1	concentrations					669:682	10% to 50% OFSP puree concentrations	647:682	10% to 50% OFSP puree concentrations	647:682	In view of developing OFSP-based functional foods to address the vitamin A deficiency (VAD) issues, herein, OFSP puree-wheat composite breads have been prepared at 10% to 50% OFSP puree concentrations and bioaccessibility of βC has been estimated.
33565638	6	76	theme	calculated	866:875	arg1	RAE					907:909	RAE	907:909	RAE	907:909	The corresponding calculated retinol activity equivalents (RAE) are 30.9, 66.4, 119.5, 170.4, and 246.2 RAE/100 g.
33565638	6	76	theme	calculated	866:875	arg1	equivalents					894:904	The corresponding calculated retinol activity equivalents	848:904	The corresponding calculated retinol activity equivalents (RAE)	848:910	The corresponding calculated retinol activity equivalents (RAE) are 30.9, 66.4, 119.5, 170.4, and 246.2 RAE/100 g.
33565638	6	76	theme	calculated	866:875	arg1	g					960:960	30.9, 66.4, 119.5, 170.4, and 246.2 RAE/100 g	916:960	30.9, 66.4, 119.5, 170.4, and 246.2 RAE/100 g	916:960	The corresponding calculated retinol activity equivalents (RAE) are 30.9, 66.4, 119.5, 170.4, and 246.2 RAE/100 g.
33565638	0	77	theme	β-carotene	25:34	arg1	bioaccessibility					36:51	β-carotene bioaccessibility	25:51	β-carotene bioaccessibility	25:51	Starch digestibility and β-carotene bioaccessibility in the orange- fleshed sweet potato puree-wheat bread.
33565638	1	78	theme	Vitamin	108:114	arg1	A					116:116	Vitamin A	108:116	Vitamin A	108:116	Vitamin A is essential for vision, human health, growth, immune function, and reproduction.
33565638	2	79	from	anemia	224:229	arg1	children					271:278	children	271:278	children	271:278	Its deficiency leads to anemia, xerophthalmia, and growth reduction in children.
33565638	9	80	theme	in	1269:1270	arg1	digestion					1285:1293	in vitro starch digestion	1269:1293	in vitro starch digestion	1269:1293	Furthermore, in vitro starch digestion decreases with significant reduction in the Rapidly Digestible Starch (RDS) amount coupled with increase in the Slowly Digestible Starch (SDS) and Resistant Starch (RS) fractions.
33565638	2	81	from	xerophthalmia	232:244	arg1	children					271:278	children	271:278	children	271:278	Its deficiency leads to anemia, xerophthalmia, and growth reduction in children.
33565638	7	82	theme	simulated	1044:1052	arg1	digestion					1090:1098	simulated oral, gastric, and small intestinal digestion	1044:1098	simulated oral, gastric, and small intestinal digestion	1044:1098	The efficiency of micellarization of all-trans-βC, 13-cis βC, and 9-cis βC after simulated oral, gastric, and small intestinal digestion are 1.4% to 6.4%, 1.4% to 7.2%, and 1.1% to 6.9%, respectively.
33565638	7	83	theme	small	1073:1077	arg1	digestion					1090:1098	simulated oral, gastric, and small intestinal digestion	1044:1098	simulated oral, gastric, and small intestinal digestion	1044:1098	The efficiency of micellarization of all-trans-βC, 13-cis βC, and 9-cis βC after simulated oral, gastric, and small intestinal digestion are 1.4% to 6.4%, 1.4% to 7.2%, and 1.1% to 6.9%, respectively.
33565638	4	84	theme	βC	708:709	arg1	bioaccessibility					688:703	bioaccessibility	688:703	bioaccessibility of βC	688:709	In view of developing OFSP-based functional foods to address the vitamin A deficiency (VAD) issues, herein, OFSP puree-wheat composite breads have been prepared at 10% to 50% OFSP puree concentrations and bioaccessibility of βC has been estimated.
33565638	11	85	theme	βC	1590:1591	arg1	amount					1566:1571	The amount	1562:1571	The amount of bioaccessible βC coupled with altered starch digestion of the OFSP wheat breads	1562:1654	The amount of bioaccessible βC coupled with altered starch digestion of the OFSP wheat breads highlight their usefulness as novel functional foods that could address the VAD as well as glycemic issues toward improving human health.
33565638	11	85	theme	βC	1590:1591	arg1	βC					1590:1591	bioaccessible βC	1576:1591	bioaccessible βC coupled with altered starch digestion of the OFSP wheat breads	1576:1654	The amount of bioaccessible βC coupled with altered starch digestion of the OFSP wheat breads highlight their usefulness as novel functional foods that could address the VAD as well as glycemic issues toward improving human health.
33565638	0	86	theme	orange-	60:66	arg1	bread					101:105	the orange- fleshed sweet potato puree-wheat bread	56:105	the orange- fleshed sweet potato puree-wheat bread	56:105	Starch digestibility and β-carotene bioaccessibility in the orange- fleshed sweet potato puree-wheat bread.
33565638	9	87	theme	starch	1278:1283	arg1	digestion					1285:1293	in vitro starch digestion	1269:1293	in vitro starch digestion	1269:1293	Furthermore, in vitro starch digestion decreases with significant reduction in the Rapidly Digestible Starch (RDS) amount coupled with increase in the Slowly Digestible Starch (SDS) and Resistant Starch (RS) fractions.
33565638	3	88	contain	have	331:334	arg2	potential					340:348	the potential	336:348	the potential	336:348	Foods enriched with naturally occurring carotenes have the potential, in this regard, and orange-fleshed sweet potato (OFSP) stands out tall as it is rich in β-carotene (βC), a provitamin A carotenoid.
33565638	3	88	contain	have	331:334	arg1	Foods					281:285	Foods	281:285	Foods enriched with naturally occurring carotenes	281:329	Foods enriched with naturally occurring carotenes have the potential, in this regard, and orange-fleshed sweet potato (OFSP) stands out tall as it is rich in β-carotene (βC), a provitamin A carotenoid.
33565638	7	89	theme	βC	1035:1036	arg1	micellarization					981:995	micellarization	981:995	micellarization of all-trans-βC, 13-cis βC, and 9-cis βC after simulated oral, gastric, and small intestinal digestion	981:1098	The efficiency of micellarization of all-trans-βC, 13-cis βC, and 9-cis βC after simulated oral, gastric, and small intestinal digestion are 1.4% to 6.4%, 1.4% to 7.2%, and 1.1% to 6.9%, respectively.
33565638	3	90	theme	orange-fleshed	371:384	arg1	OFSP					400:403	OFSP	400:403	OFSP	400:403	Foods enriched with naturally occurring carotenes have the potential, in this regard, and orange-fleshed sweet potato (OFSP) stands out tall as it is rich in β-carotene (βC), a provitamin A carotenoid.
33565638	3	90	theme	orange-fleshed	371:384	arg1	potato					392:397	orange-fleshed sweet potato	371:397	orange-fleshed sweet potato (OFSP)	371:404	Foods enriched with naturally occurring carotenes have the potential, in this regard, and orange-fleshed sweet potato (OFSP) stands out tall as it is rich in β-carotene (βC), a provitamin A carotenoid.
33565638	10	91	theme	carotenoids	1549:1559	arg1	amount					1526:1531	adequate amount	1517:1531	adequate amount of provitamin A carotenoids	1517:1559	Overall, OFSP-wheat composite bread holds adequate amount of provitamin A carotenoids.
33565638	10	91	theme	carotenoids	1549:1559	arg1	carotenoids					1549:1559	provitamin A carotenoids	1536:1559	provitamin A carotenoids	1536:1559	Overall, OFSP-wheat composite bread holds adequate amount of provitamin A carotenoids.
33565638	7	92	dep	%	1147:1147	arg1	to					1141:1142	to	1141:1142	to	1141:1142	The efficiency of micellarization of all-trans-βC, 13-cis βC, and 9-cis βC after simulated oral, gastric, and small intestinal digestion are 1.4% to 6.4%, 1.4% to 7.2%, and 1.1% to 6.9%, respectively.
33565638	10	93	theme	provitamin	1536:1545	arg1	carotenoids					1549:1559	provitamin A carotenoids	1536:1559	provitamin A carotenoids	1536:1559	Overall, OFSP-wheat composite bread holds adequate amount of provitamin A carotenoids.
33565638	11	94	theme	breads	1649:1654	arg1	digestion					1621:1629	altered starch digestion	1606:1629	altered starch digestion of the OFSP wheat breads	1606:1654	The amount of bioaccessible βC coupled with altered starch digestion of the OFSP wheat breads highlight their usefulness as novel functional foods that could address the VAD as well as glycemic issues toward improving human health.
33565638	9	95	theme	significant	1310:1320	arg1	reduction					1322:1330	significant reduction	1310:1330	significant reduction in the Rapidly Digestible Starch (RDS) amount coupled with increase in the Slowly Digestible Starch (SDS) and Resistant Starch (RS) fractions	1310:1472	Furthermore, in vitro starch digestion decreases with significant reduction in the Rapidly Digestible Starch (RDS) amount coupled with increase in the Slowly Digestible Starch (SDS) and Resistant Starch (RS) fractions.
33565638	7	96	theme	βC	1021:1022	arg1	micellarization					981:995	micellarization	981:995	micellarization of all-trans-βC, 13-cis βC, and 9-cis βC after simulated oral, gastric, and small intestinal digestion	981:1098	The efficiency of micellarization of all-trans-βC, 13-cis βC, and 9-cis βC after simulated oral, gastric, and small intestinal digestion are 1.4% to 6.4%, 1.4% to 7.2%, and 1.1% to 6.9%, respectively.
33565638	7	97	theme	intestinal	1079:1088	arg1	digestion					1090:1098	simulated oral, gastric, and small intestinal digestion	1044:1098	simulated oral, gastric, and small intestinal digestion	1044:1098	The efficiency of micellarization of all-trans-βC, 13-cis βC, and 9-cis βC after simulated oral, gastric, and small intestinal digestion are 1.4% to 6.4%, 1.4% to 7.2%, and 1.1% to 6.9%, respectively.
33565638	4	98	theme	vitamin	548:554	arg1	VAD					570:572	VAD	570:572	VAD	570:572	In view of developing OFSP-based functional foods to address the vitamin A deficiency (VAD) issues, herein, OFSP puree-wheat composite breads have been prepared at 10% to 50% OFSP puree concentrations and bioaccessibility of βC has been estimated.
33565638	4	98	theme	vitamin	548:554	arg1	deficiency					558:567	the vitamin A deficiency	544:567	the vitamin A deficiency (VAD) issues	544:580	In view of developing OFSP-based functional foods to address the vitamin A deficiency (VAD) issues, herein, OFSP puree-wheat composite breads have been prepared at 10% to 50% OFSP puree concentrations and bioaccessibility of βC has been estimated.
32673710	12	0	theme	uncultured	2069:2078	arg1	Senegalimassilia					2051:2066	Senegalimassilia	2051:2066	Senegalimassilia	2051:2066	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	12	0	theme	uncultured	2069:2078	arg1	bacteria					2080:2087	uncultured bacteria	2069:2087	uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group	2069:2148	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	6	1	theme	anaerobic	1087:1095	arg1	cultures					1097:1104	Ex vivo anaerobic cultures	1079:1104	Ex vivo anaerobic cultures of piglets intestinal microbiota	1079:1137	MATERIALS AND METHODS Ex vivo anaerobic cultures of piglets intestinal microbiota obtained from jejunum, ileum, caecum and distal colon were conducted in various culture media supplemented with LSH.
32673710	12	2	contain	had	1893:1895	arg2	influence					1912:1920	no significant influence	1897:1920	no significant influence	1897:1920	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	12	2	contain	had	1893:1895	arg1	LSH					1889:1891	LSH	1889:1891	LSH	1889:1891	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	14	3	theme	diarrhea	2900:2907	arg1	prevention					2873:2882	prevention	2873:2882	prevention	2873:2882	CONCLUSIONS In the present study we have shown, that Lythrum salicaria herb fulfills the criteria of a potential candidate for antidiarrheal agent, which could be applied as therapy or prevention of post-weaning diarrhea in piglets.
32673710	14	3	theme	diarrhea	2900:2907	arg1	therapy					2862:2868	therapy	2862:2868	therapy	2862:2868	CONCLUSIONS In the present study we have shown, that Lythrum salicaria herb fulfills the criteria of a potential candidate for antidiarrheal agent, which could be applied as therapy or prevention of post-weaning diarrhea in piglets.
32673710	14	3	theme	diarrhea	2900:2907	arg1	candidate					2801:2809	a potential candidate	2789:2809	a potential candidate	2789:2809	CONCLUSIONS In the present study we have shown, that Lythrum salicaria herb fulfills the criteria of a potential candidate for antidiarrheal agent, which could be applied as therapy or prevention of post-weaning diarrhea in piglets.
32673710	6	4	theme	distal	1180:1185	arg1	colon					1187:1191	distal colon	1180:1191	distal colon	1180:1191	MATERIALS AND METHODS Ex vivo anaerobic cultures of piglets intestinal microbiota obtained from jejunum, ileum, caecum and distal colon were conducted in various culture media supplemented with LSH.
32673710	10	5	theme	distal	1593:1598	arg1	able					1621:1624	able	1621:1624	able	1621:1624	RESULTS Only the caecal and distal colon microbiota was able to hydrolyze and metabolize ellagitannins present in LSH to urolithins.
32673710	10	5	theme	distal	1593:1598	arg1	microbiota					1606:1615	Only the caecal and distal colon microbiota	1573:1615	Only the caecal and distal colon microbiota	1573:1615	RESULTS Only the caecal and distal colon microbiota was able to hydrolyze and metabolize ellagitannins present in LSH to urolithins.
32673710	11	6	theme	Urolithin	1698:1706	arg1	M6					1708:1709	Urolithin M6	1698:1709	Urolithin M6	1698:1709	Urolithin M6, M7, urolithin C, A and iso-urolithin A were detected together with a previously not described metabolite originating from the flavogalloyl moiety of C-glucosylic ellagitannins.
32673710	13	7	theme	urolithins	2660:2669	arg1	production					2646:2655	production	2646:2655	production of urolithins	2646:2669	Significant correlations between taxa abundance and production of urolithins were determined.
32673710	13	7	theme	urolithins	2660:2669	arg1	abundance					2632:2640	taxa abundance	2627:2640	taxa abundance	2627:2640	Significant correlations between taxa abundance and production of urolithins were determined.
32673710	10	8	theme	caecal	1582:1587	arg1	able					1621:1624	able	1621:1624	able	1621:1624	RESULTS Only the caecal and distal colon microbiota was able to hydrolyze and metabolize ellagitannins present in LSH to urolithins.
32673710	10	8	theme	caecal	1582:1587	arg1	microbiota					1606:1615	Only the caecal and distal colon microbiota	1573:1615	Only the caecal and distal colon microbiota	1573:1615	RESULTS Only the caecal and distal colon microbiota was able to hydrolyze and metabolize ellagitannins present in LSH to urolithins.
32673710	3	9	theme	economic	520:527	arg1	losses					529:534	significant economic losses	508:534	significant economic losses	508:534	Post-weaning diarrhea is a main problem affecting global piglet production, which leads to significant economic losses because of increased morbidity and mortality, reduced average daily gain, and high antibiotic consumption.
32673710	5	10	theme	gut	833:835	arg1	microbiota					837:846	the gut microbiota	829:846	the gut microbiota of healthy post-weaning piglets	829:878	The aim of the present study was to determine the interaction of LSH with the gut microbiota of healthy post-weaning piglets in order to evaluate its influence on microbiota composition and metabolism as well as production of potentially bioactive postbiotic metabolites from the extract constituents.
32673710	4	11	theme	various	669:675	arg1	causes					677:682	various causes	669:682	various causes	669:682	Post-weaning diarrhea has various causes, all of which have been linked to imbalances of intestinal microbiota.
32673710	14	12	theme	post-weaning	2887:2898	arg1	diarrhea					2900:2907	post-weaning diarrhea	2887:2907	post-weaning diarrhea in piglets	2887:2918	CONCLUSIONS In the present study we have shown, that Lythrum salicaria herb fulfills the criteria of a potential candidate for antidiarrheal agent, which could be applied as therapy or prevention of post-weaning diarrhea in piglets.
32673710	2	13	dep	Lythrum	228:234	arg1	L.					246:247	Lythrum salicaria L.	228:247	Lythrum salicaria L. from Lythraceae family	228:270	ETHNOPHARMACOLOGICAL RELEVANCE Herb of purple loosestrife (Lythrum salicaria L. from Lythraceae family) (LSH) was used in Europe since ancient times till early-20th century in the therapy of diarrhea and dysentery in human and veterinary medicine.
32673710	15	14	theme	microbiota	2951:2960	arg1	composition					2962:2972	the gut microbiota composition	2943:2972	the gut microbiota composition	2943:2972	It not only modulates the gut microbiota composition without causing the dysbiosis and impairing metabolic activity, but is also a source of postbiotic metabolites, namely urolithins, which anti-inflammatory properties can be beneficial for gut health of piglets during the weaning period.
32673710	3	15	theme	increased	547:555	arg1	morbidity					557:565	morbidity	557:565	morbidity	557:565	Post-weaning diarrhea is a main problem affecting global piglet production, which leads to significant economic losses because of increased morbidity and mortality, reduced average daily gain, and high antibiotic consumption.
32673710	11	16	theme	flavogalloyl	1838:1849	arg1	moiety					1851:1856	the flavogalloyl moiety	1834:1856	the flavogalloyl moiety of C-glucosylic ellagitannins	1834:1886	Urolithin M6, M7, urolithin C, A and iso-urolithin A were detected together with a previously not described metabolite originating from the flavogalloyl moiety of C-glucosylic ellagitannins.
32673710	13	17	theme	Significant	2594:2604	arg1	correlations					2606:2617	Significant correlations	2594:2617	Significant correlations between taxa abundance and production of urolithins	2594:2669	Significant correlations between taxa abundance and production of urolithins were determined.
32673710	3	18	theme	average	590:596	arg1	gain					604:607	reduced average daily gain	582:607	reduced average daily gain	582:607	Post-weaning diarrhea is a main problem affecting global piglet production, which leads to significant economic losses because of increased morbidity and mortality, reduced average daily gain, and high antibiotic consumption.
32673710	12	19	theme	Lachnospiraceae	2476:2490	arg1	group					2469:2473	Prevotella, Agathobacter, [Eubacterium] hallii group	2422:2473	group	2469:2473	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	12	19	theme	Lachnospiraceae	2476:2490	arg1	group					2499:2503	Lachnospiraceae NK3A20 group	2476:2503	Lachnospiraceae NK3A20 group	2476:2503	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	8	20	theme	bioinformatic	1475:1487	arg1	analysis					1489:1496	bioinformatic analysis	1475:1496	bioinformatic analysis	1475:1496	Bacterial genomic DNA was extracted and examined by sequencing by amplification of the 16S rDNA V3-V4 hypervariable regions followed by bioinformatic analysis.
32673710	6	21	theme	culture	1219:1225	arg1	media					1227:1231	various culture media	1211:1231	various culture media supplemented with LSH	1211:1253	MATERIALS AND METHODS Ex vivo anaerobic cultures of piglets intestinal microbiota obtained from jejunum, ileum, caecum and distal colon were conducted in various culture media supplemented with LSH.
32673710	12	22	theme	Rikenellaceae	2122:2134	arg1	group					2144:2148	Porphyromonadaceae, Rikenellaceae RC9 gut group	2102:2148	Porphyromonadaceae, Rikenellaceae RC9 gut group	2102:2148	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	12	23	theme	gut	2140:2142	arg1	group					2144:2148	Porphyromonadaceae, Rikenellaceae RC9 gut group	2102:2148	Porphyromonadaceae, Rikenellaceae RC9 gut group	2102:2148	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	5	24	theme	microbiota	918:927	arg1	composition					929:939	microbiota composition	918:939	microbiota composition	918:939	The aim of the present study was to determine the interaction of LSH with the gut microbiota of healthy post-weaning piglets in order to evaluate its influence on microbiota composition and metabolism as well as production of potentially bioactive postbiotic metabolites from the extract constituents.
32673710	12	25	dep	group	2469:2473	arg1	[Eubacterium					2448:2459	Prevotella, Agathobacter, [Eubacterium] hallii group	2422:2473	[Eubacterium	2448:2459	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	1	26	theme	postbiotic	125:134	arg1	metabolites					136:146	postbiotic metabolites	125:146	postbiotic metabolites	125:146	Focus on prebiotic properties and formation of postbiotic metabolites in ex vivo cultures.
32673710	14	27	theme	present	2707:2713	arg1	study					2715:2719	the present study	2703:2719	the present study	2703:2719	CONCLUSIONS In the present study we have shown, that Lythrum salicaria herb fulfills the criteria of a potential candidate for antidiarrheal agent, which could be applied as therapy or prevention of post-weaning diarrhea in piglets.
32673710	3	28	theme	main	444:447	arg1	diarrhea					430:437	Post-weaning diarrhea	417:437	Post-weaning diarrhea	417:437	Post-weaning diarrhea is a main problem affecting global piglet production, which leads to significant economic losses because of increased morbidity and mortality, reduced average daily gain, and high antibiotic consumption.
32673710	3	28	theme	main	444:447	arg1	problem					449:455	a main problem	442:455	a main problem affecting global piglet production, which leads to significant economic losses because of increased morbidity and mortality, reduced average daily gain, and high antibiotic consumption	442:640	Post-weaning diarrhea is a main problem affecting global piglet production, which leads to significant economic losses because of increased morbidity and mortality, reduced average daily gain, and high antibiotic consumption.
32673710	11	29	theme	iso-urolithin	1735:1747	arg1	A					1749:1749	iso-urolithin A	1735:1749	iso-urolithin A	1735:1749	Urolithin M6, M7, urolithin C, A and iso-urolithin A were detected together with a previously not described metabolite originating from the flavogalloyl moiety of C-glucosylic ellagitannins.
32673710	0	30	theme	healthy	48:54	arg1	piglets					69:75	healthy post-weaning piglets	48:75	healthy post-weaning piglets	48:75	Lythrum salicaria L. herb and gut microbiota of healthy post-weaning piglets.
32673710	12	31	theme	Prevotella	2422:2431	arg1	Catenisphaera					2553:2565	Catenisphaera	2553:2565	Catenisphaera	2553:2565	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	12	31	theme	Prevotella	2422:2431	arg1	group					2469:2473	Prevotella, Agathobacter, [Eubacterium] hallii group	2422:2473	group	2469:2473	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	12	31	theme	Prevotella	2422:2431	arg1	Agathobacter					2434:2445	Agathobacter	2434:2445	Agathobacter	2434:2445	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	12	31	theme	Prevotella	2422:2431	arg1	group					2529:2533	[Ruminococcus] torques group	2506:2533	[Ruminococcus] torques group	2506:2533	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	12	31	theme	Prevotella	2422:2431	arg1	Megasphaera					2571:2581	Megasphaera	2571:2581	Megasphaera	2571:2581	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	12	31	theme	Prevotella	2422:2431	arg1	group					2499:2503	Lachnospiraceae NK3A20 group	2476:2503	Lachnospiraceae NK3A20 group	2476:2503	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	5	32	theme	post-weaning	859:870	arg1	piglets					872:878	healthy post-weaning piglets	851:878	healthy post-weaning piglets	851:878	The aim of the present study was to determine the interaction of LSH with the gut microbiota of healthy post-weaning piglets in order to evaluate its influence on microbiota composition and metabolism as well as production of potentially bioactive postbiotic metabolites from the extract constituents.
32673710	2	33	from	medicine	407:414	arg1	therapy					349:355	the therapy	345:355	the therapy of diarrhea and dysentery in human and veterinary medicine	345:414	ETHNOPHARMACOLOGICAL RELEVANCE Herb of purple loosestrife (Lythrum salicaria L. from Lythraceae family) (LSH) was used in Europe since ancient times till early-20th century in the therapy of diarrhea and dysentery in human and veterinary medicine.
32673710	8	34	theme	genomic	1349:1355	arg1	DNA					1357:1359	Bacterial genomic DNA	1339:1359	Bacterial genomic DNA	1339:1359	Bacterial genomic DNA was extracted and examined by sequencing by amplification of the 16S rDNA V3-V4 hypervariable regions followed by bioinformatic analysis.
32673710	0	35	theme	piglets	69:75	arg1	gut					30:32	gut	30:32	gut	30:32	Lythrum salicaria L. herb and gut microbiota of healthy post-weaning piglets.
32673710	0	35	theme	piglets	69:75	arg1	herb					21:24	herb	21:24	herb	21:24	Lythrum salicaria L. herb and gut microbiota of healthy post-weaning piglets.
32673710	15	36	theme	metabolic	3018:3026	arg1	activity					3028:3035	metabolic activity	3018:3035	metabolic activity	3018:3035	It not only modulates the gut microbiota composition without causing the dysbiosis and impairing metabolic activity, but is also a source of postbiotic metabolites, namely urolithins, which anti-inflammatory properties can be beneficial for gut health of piglets during the weaning period.
32673710	12	37	dep	coprostanoligenes	2247:2263	arg1	[Eubacterium					2233:2244	Moryella, [Eubacterium] coprostanoligenes	2223:2263	[Eubacterium	2233:2244	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	15	38	theme	gut	3162:3164	arg1	health					3166:3171	gut health	3162:3171	gut health of piglets	3162:3182	It not only modulates the gut microbiota composition without causing the dysbiosis and impairing metabolic activity, but is also a source of postbiotic metabolites, namely urolithins, which anti-inflammatory properties can be beneficial for gut health of piglets during the weaning period.
32673710	12	39	theme	uncultured	2307:2316	arg1	Senegalimassilia					2051:2066	Senegalimassilia	2051:2066	Senegalimassilia	2051:2066	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	12	39	theme	uncultured	2307:2316	arg1	bacteria					2318:2325	uncultured bacteria	2307:2325	uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella	2307:2387	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	2	40	theme	dysentery	373:381	arg1	therapy					349:355	the therapy	345:355	the therapy of diarrhea and dysentery in human and veterinary medicine	345:414	ETHNOPHARMACOLOGICAL RELEVANCE Herb of purple loosestrife (Lythrum salicaria L. from Lythraceae family) (LSH) was used in Europe since ancient times till early-20th century in the therapy of diarrhea and dysentery in human and veterinary medicine.
32673710	15	41	theme	anti-inflammatory	3111:3127	arg1	properties					3129:3138	anti-inflammatory properties	3111:3138	anti-inflammatory properties	3111:3138	It not only modulates the gut microbiota composition without causing the dysbiosis and impairing metabolic activity, but is also a source of postbiotic metabolites, namely urolithins, which anti-inflammatory properties can be beneficial for gut health of piglets during the weaning period.
32673710	9	42	theme	GC	1552:1553	arg1	analysis					1555:1562	GC analysis	1552:1562	GC analysis	1552:1562	The production of SCFA in cultures was determined by GC analysis.
32673710	10	43	attach	present	1668:1674	arg2	ellagitannins					1654:1666	ellagitannins	1654:1666	ellagitannins present in LSH to urolithins	1654:1695	RESULTS Only the caecal and distal colon microbiota was able to hydrolyze and metabolize ellagitannins present in LSH to urolithins.
32673710	10	43	attach	present	1668:1674	arg1	LSH					1679:1681	LSH	1679:1681	LSH to urolithins	1679:1695	RESULTS Only the caecal and distal colon microbiota was able to hydrolyze and metabolize ellagitannins present in LSH to urolithins.
32673710	0	44	theme	post-weaning	56:67	arg1	piglets					69:75	healthy post-weaning piglets	48:75	healthy post-weaning piglets	48:75	Lythrum salicaria L. herb and gut microbiota of healthy post-weaning piglets.
32673710	1	45	dep	ex	151:152	arg1	vivo					154:157	vivo	154:157	vivo	154:157	Focus on prebiotic properties and formation of postbiotic metabolites in ex vivo cultures.
32673710	2	46	theme	diarrhea	360:367	arg1	therapy					349:355	the therapy	345:355	the therapy of diarrhea and dysentery in human and veterinary medicine	345:414	ETHNOPHARMACOLOGICAL RELEVANCE Herb of purple loosestrife (Lythrum salicaria L. from Lythraceae family) (LSH) was used in Europe since ancient times till early-20th century in the therapy of diarrhea and dysentery in human and veterinary medicine.
32673710	12	47	theme	microbiota	1925:1934	arg1	diversity					1936:1944	microbiota diversity	1925:1944	microbiota diversity	1925:1944	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	11	48	theme	urolithin	1716:1724	arg1	C					1726:1726	urolithin C	1716:1726	urolithin C	1716:1726	Urolithin M6, M7, urolithin C, A and iso-urolithin A were detected together with a previously not described metabolite originating from the flavogalloyl moiety of C-glucosylic ellagitannins.
32673710	2	49	theme	Lythraceae	254:263	arg1	family					265:270	Lythraceae family	254:270	Lythraceae family	254:270	ETHNOPHARMACOLOGICAL RELEVANCE Herb of purple loosestrife (Lythrum salicaria L. from Lythraceae family) (LSH) was used in Europe since ancient times till early-20th century in the therapy of diarrhea and dysentery in human and veterinary medicine.
32673710	14	50	theme	candidate	2801:2809	arg1	criteria					2777:2784	the criteria	2773:2784	the criteria of a potential candidate for antidiarrheal agent, which could be applied as therapy or prevention of post-weaning diarrhea in piglets	2773:2918	CONCLUSIONS In the present study we have shown, that Lythrum salicaria herb fulfills the criteria of a potential candidate for antidiarrheal agent, which could be applied as therapy or prevention of post-weaning diarrhea in piglets.
32673710	4	51	theme	intestinal	732:741	arg1	microbiota					743:752	intestinal microbiota	732:752	intestinal microbiota	732:752	Post-weaning diarrhea has various causes, all of which have been linked to imbalances of intestinal microbiota.
32673710	2	52	theme	veterinary	396:405	arg1	medicine					407:414	human and veterinary medicine	386:414	human and veterinary medicine	386:414	ETHNOPHARMACOLOGICAL RELEVANCE Herb of purple loosestrife (Lythrum salicaria L. from Lythraceae family) (LSH) was used in Europe since ancient times till early-20th century in the therapy of diarrhea and dysentery in human and veterinary medicine.
32673710	6	53	dep	Ex	1079:1080	arg1	vivo					1082:1085	vivo	1082:1085	vivo	1082:1085	MATERIALS AND METHODS Ex vivo anaerobic cultures of piglets intestinal microbiota obtained from jejunum, ileum, caecum and distal colon were conducted in various culture media supplemented with LSH.
32673710	7	54	theme	UPLC-DAD-MSn	1318:1329	arg1	method					1331:1336	UPLC-DAD-MSn method	1318:1336	UPLC-DAD-MSn method	1318:1336	The production of postbiotic metabolites was determined using UPLC-DAD-MSn method.
32673710	5	55	with	interaction	805:815	arg1	microbiota					837:846	the gut microbiota	829:846	the gut microbiota of healthy post-weaning piglets	829:878	The aim of the present study was to determine the interaction of LSH with the gut microbiota of healthy post-weaning piglets in order to evaluate its influence on microbiota composition and metabolism as well as production of potentially bioactive postbiotic metabolites from the extract constituents.
32673710	2	56	theme	ETHNOPHARMACOLOGICAL	169:188	arg1	Herb					200:203	ETHNOPHARMACOLOGICAL RELEVANCE Herb	169:203	ETHNOPHARMACOLOGICAL RELEVANCE Herb of purple loosestrife (Lythrum salicaria L. from Lythraceae family) (LSH)	169:277	ETHNOPHARMACOLOGICAL RELEVANCE Herb of purple loosestrife (Lythrum salicaria L. from Lythraceae family) (LSH) was used in Europe since ancient times till early-20th century in the therapy of diarrhea and dysentery in human and veterinary medicine.
32673710	2	57	theme	human	386:390	arg1	medicine					407:414	human and veterinary medicine	386:414	human and veterinary medicine	386:414	ETHNOPHARMACOLOGICAL RELEVANCE Herb of purple loosestrife (Lythrum salicaria L. from Lythraceae family) (LSH) was used in Europe since ancient times till early-20th century in the therapy of diarrhea and dysentery in human and veterinary medicine.
32673710	5	58	theme	postbiotic	1003:1012	arg1	metabolites					1014:1024	potentially bioactive postbiotic metabolites	981:1024	potentially bioactive postbiotic metabolites from the extract constituents	981:1054	The aim of the present study was to determine the interaction of LSH with the gut microbiota of healthy post-weaning piglets in order to evaluate its influence on microbiota composition and metabolism as well as production of potentially bioactive postbiotic metabolites from the extract constituents.
32673710	2	59	from	diarrhea	360:367	arg1	medicine					407:414	human and veterinary medicine	386:414	human and veterinary medicine	386:414	ETHNOPHARMACOLOGICAL RELEVANCE Herb of purple loosestrife (Lythrum salicaria L. from Lythraceae family) (LSH) was used in Europe since ancient times till early-20th century in the therapy of diarrhea and dysentery in human and veterinary medicine.
32673710	14	60	theme	antidiarrheal	2815:2827	arg1	agent					2829:2833	antidiarrheal agent	2815:2833	antidiarrheal agent	2815:2833	CONCLUSIONS In the present study we have shown, that Lythrum salicaria herb fulfills the criteria of a potential candidate for antidiarrheal agent, which could be applied as therapy or prevention of post-weaning diarrhea in piglets.
32673710	12	61	theme	NK3A20	2492:2497	arg1	group					2469:2473	Prevotella, Agathobacter, [Eubacterium] hallii group	2422:2473	group	2469:2473	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	12	61	theme	NK3A20	2492:2497	arg1	group					2499:2503	Lachnospiraceae NK3A20 group	2476:2503	Lachnospiraceae NK3A20 group	2476:2503	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	3	62	theme	antibiotic	619:628	arg1	consumption					630:640	high antibiotic consumption	614:640	high antibiotic consumption	614:640	Post-weaning diarrhea is a main problem affecting global piglet production, which leads to significant economic losses because of increased morbidity and mortality, reduced average daily gain, and high antibiotic consumption.
32673710	8	63	theme	16S	1426:1428	arg1	regions					1455:1461	the 16S rDNA V3-V4 hypervariable regions	1422:1461	the 16S rDNA V3-V4 hypervariable regions followed by bioinformatic analysis	1422:1496	Bacterial genomic DNA was extracted and examined by sequencing by amplification of the 16S rDNA V3-V4 hypervariable regions followed by bioinformatic analysis.
32673710	3	64	theme	piglet	474:479	arg1	production					481:490	global piglet production	467:490	global piglet production	467:490	Post-weaning diarrhea is a main problem affecting global piglet production, which leads to significant economic losses because of increased morbidity and mortality, reduced average daily gain, and high antibiotic consumption.
32673710	5	65	theme	study	778:782	arg1	aim					759:761	The aim	755:761	The aim of the present study	755:782	The aim of the present study was to determine the interaction of LSH with the gut microbiota of healthy post-weaning piglets in order to evaluate its influence on microbiota composition and metabolism as well as production of potentially bioactive postbiotic metabolites from the extract constituents.
32673710	2	66	from	dysentery	373:381	arg1	medicine					407:414	human and veterinary medicine	386:414	human and veterinary medicine	386:414	ETHNOPHARMACOLOGICAL RELEVANCE Herb of purple loosestrife (Lythrum salicaria L. from Lythraceae family) (LSH) was used in Europe since ancient times till early-20th century in the therapy of diarrhea and dysentery in human and veterinary medicine.
32673710	6	67	theme	microbiota	1128:1137	arg1	cultures					1097:1104	Ex vivo anaerobic cultures	1079:1104	Ex vivo anaerobic cultures of piglets intestinal microbiota	1079:1137	MATERIALS AND METHODS Ex vivo anaerobic cultures of piglets intestinal microbiota obtained from jejunum, ileum, caecum and distal colon were conducted in various culture media supplemented with LSH.
32673710	2	68	used	used	283:286	arg2	Herb					200:203	ETHNOPHARMACOLOGICAL RELEVANCE Herb	169:203	ETHNOPHARMACOLOGICAL RELEVANCE Herb of purple loosestrife (Lythrum salicaria L. from Lythraceae family) (LSH)	169:277	ETHNOPHARMACOLOGICAL RELEVANCE Herb of purple loosestrife (Lythrum salicaria L. from Lythraceae family) (LSH) was used in Europe since ancient times till early-20th century in the therapy of diarrhea and dysentery in human and veterinary medicine.
32673710	2	69	theme	purple	208:213	arg1	LSH					274:276	LSH	274:276	LSH	274:276	ETHNOPHARMACOLOGICAL RELEVANCE Herb of purple loosestrife (Lythrum salicaria L. from Lythraceae family) (LSH) was used in Europe since ancient times till early-20th century in the therapy of diarrhea and dysentery in human and veterinary medicine.
32673710	2	69	theme	purple	208:213	arg1	loosestrife					215:225	purple loosestrife	208:225	purple loosestrife (Lythrum salicaria L. from Lythraceae family) (LSH)	208:277	ETHNOPHARMACOLOGICAL RELEVANCE Herb of purple loosestrife (Lythrum salicaria L. from Lythraceae family) (LSH) was used in Europe since ancient times till early-20th century in the therapy of diarrhea and dysentery in human and veterinary medicine.
32673710	8	70	theme	V3-V4	1435:1439	arg1	regions					1455:1461	the 16S rDNA V3-V4 hypervariable regions	1422:1461	the 16S rDNA V3-V4 hypervariable regions followed by bioinformatic analysis	1422:1496	Bacterial genomic DNA was extracted and examined by sequencing by amplification of the 16S rDNA V3-V4 hypervariable regions followed by bioinformatic analysis.
32673710	2	71	theme	times	312:316	arg1	century					334:340	ancient times till early-20th century	304:340	ancient times till early-20th century	304:340	ETHNOPHARMACOLOGICAL RELEVANCE Herb of purple loosestrife (Lythrum salicaria L. from Lythraceae family) (LSH) was used in Europe since ancient times till early-20th century in the therapy of diarrhea and dysentery in human and veterinary medicine.
32673710	1	72	from	properties	97:106	arg1	cultures					159:166	ex vivo cultures	151:166	ex vivo cultures	151:166	Focus on prebiotic properties and formation of postbiotic metabolites in ex vivo cultures.
32673710	0	73	dep	L.	18:19	arg1	gut					30:32	gut	30:32	gut	30:32	Lythrum salicaria L. herb and gut microbiota of healthy post-weaning piglets.
32673710	0	73	dep	L.	18:19	arg1	herb					21:24	herb	21:24	herb	21:24	Lythrum salicaria L. herb and gut microbiota of healthy post-weaning piglets.
32673710	6	74	theme	piglets	1109:1115	arg1	microbiota					1128:1137	piglets intestinal microbiota	1109:1137	piglets intestinal microbiota	1109:1137	MATERIALS AND METHODS Ex vivo anaerobic cultures of piglets intestinal microbiota obtained from jejunum, ileum, caecum and distal colon were conducted in various culture media supplemented with LSH.
32673710	12	75	theme	hallii	2462:2467	arg1	Catenisphaera					2553:2565	Catenisphaera	2553:2565	Catenisphaera	2553:2565	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	12	75	theme	hallii	2462:2467	arg1	group					2469:2473	Prevotella, Agathobacter, [Eubacterium] hallii group	2422:2473	group	2469:2473	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	12	75	theme	hallii	2462:2467	arg1	Agathobacter					2434:2445	Agathobacter	2434:2445	Agathobacter	2434:2445	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	12	75	theme	hallii	2462:2467	arg1	group					2529:2533	[Ruminococcus] torques group	2506:2533	[Ruminococcus] torques group	2506:2533	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	12	75	theme	hallii	2462:2467	arg1	Megasphaera					2571:2581	Megasphaera	2571:2581	Megasphaera	2571:2581	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	12	75	theme	hallii	2462:2467	arg1	group					2499:2503	Lachnospiraceae NK3A20 group	2476:2503	Lachnospiraceae NK3A20 group	2476:2503	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	15	76	theme	metabolites	3073:3083	arg1	source					3052:3057	a source	3050:3057	a source of postbiotic metabolites, namely urolithins, which anti-inflammatory properties can be beneficial for gut health of piglets during the weaning period	3050:3208	It not only modulates the gut microbiota composition without causing the dysbiosis and impairing metabolic activity, but is also a source of postbiotic metabolites, namely urolithins, which anti-inflammatory properties can be beneficial for gut health of piglets during the weaning period.
32673710	8	77	theme	regions	1455:1461	arg1	amplification					1405:1417	amplification	1405:1417	amplification of the 16S rDNA V3-V4 hypervariable regions followed by bioinformatic analysis	1405:1496	Bacterial genomic DNA was extracted and examined by sequencing by amplification of the 16S rDNA V3-V4 hypervariable regions followed by bioinformatic analysis.
32673710	14	78	from	therapy	2862:2868	arg1	piglets					2912:2918	piglets	2912:2918	piglets	2912:2918	CONCLUSIONS In the present study we have shown, that Lythrum salicaria herb fulfills the criteria of a potential candidate for antidiarrheal agent, which could be applied as therapy or prevention of post-weaning diarrhea in piglets.
32673710	14	79	from	prevention	2873:2882	arg1	piglets					2912:2918	piglets	2912:2918	piglets	2912:2918	CONCLUSIONS In the present study we have shown, that Lythrum salicaria herb fulfills the criteria of a potential candidate for antidiarrheal agent, which could be applied as therapy or prevention of post-weaning diarrhea in piglets.
32673710	10	80	theme	colon	1600:1604	arg1	able					1621:1624	able	1621:1624	able	1621:1624	RESULTS Only the caecal and distal colon microbiota was able to hydrolyze and metabolize ellagitannins present in LSH to urolithins.
32673710	10	80	theme	colon	1600:1604	arg1	microbiota					1606:1615	Only the caecal and distal colon microbiota	1573:1615	Only the caecal and distal colon microbiota	1573:1615	RESULTS Only the caecal and distal colon microbiota was able to hydrolyze and metabolize ellagitannins present in LSH to urolithins.
32673710	4	81	attach	linked	708:713	arg2	causes					677:682	various causes	669:682	various causes	669:682	Post-weaning diarrhea has various causes, all of which have been linked to imbalances of intestinal microbiota.
32673710	4	81	attach	linked	708:713	arg1	imbalances					718:727	imbalances	718:727	imbalances of intestinal microbiota	718:752	Post-weaning diarrhea has various causes, all of which have been linked to imbalances of intestinal microbiota.
32673710	4	81	attach	linked	708:713	arg2	all					685:687	all	685:687	all	685:687	Post-weaning diarrhea has various causes, all of which have been linked to imbalances of intestinal microbiota.
32673710	12	82	theme	significant	2014:2024	arg1	decrease					2026:2033	significant decrease	2014:2033	significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella	2014:2387	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	11	83	theme	C-glucosylic	1861:1872	arg1	ellagitannins					1874:1886	C-glucosylic ellagitannins	1861:1886	C-glucosylic ellagitannins	1861:1886	Urolithin M6, M7, urolithin C, A and iso-urolithin A were detected together with a previously not described metabolite originating from the flavogalloyl moiety of C-glucosylic ellagitannins.
32673710	5	84	theme	piglets	872:878	arg1	microbiota					837:846	the gut microbiota	829:846	the gut microbiota of healthy post-weaning piglets	829:878	The aim of the present study was to determine the interaction of LSH with the gut microbiota of healthy post-weaning piglets in order to evaluate its influence on microbiota composition and metabolism as well as production of potentially bioactive postbiotic metabolites from the extract constituents.
32673710	14	85	from	CONCLUSIONS	2688:2698	arg1	study					2715:2719	the present study	2703:2719	the present study	2703:2719	CONCLUSIONS In the present study we have shown, that Lythrum salicaria herb fulfills the criteria of a potential candidate for antidiarrheal agent, which could be applied as therapy or prevention of post-weaning diarrhea in piglets.
32673710	2	86	theme	early-20th	323:332	arg1	century					334:340	ancient times till early-20th century	304:340	ancient times till early-20th century	304:340	ETHNOPHARMACOLOGICAL RELEVANCE Herb of purple loosestrife (Lythrum salicaria L. from Lythraceae family) (LSH) was used in Europe since ancient times till early-20th century in the therapy of diarrhea and dysentery in human and veterinary medicine.
32673710	3	87	theme	significant	508:518	arg1	losses					529:534	significant economic losses	508:534	significant economic losses	508:534	Post-weaning diarrhea is a main problem affecting global piglet production, which leads to significant economic losses because of increased morbidity and mortality, reduced average daily gain, and high antibiotic consumption.
32673710	12	88	from	decrease	2026:2033	arg1	Intestinimonas					2266:2279	Intestinimonas	2266:2279	Intestinimonas	2266:2279	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	12	88	from	decrease	2026:2033	arg1	bacteria					2318:2325	uncultured bacteria	2307:2325	uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella	2307:2387	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	12	88	from	decrease	2026:2033	arg1	bacteria					2080:2087	uncultured bacteria	2069:2087	uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group	2069:2148	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	12	88	from	decrease	2026:2033	arg1	Ruminococcaceae					2282:2296	Ruminococcaceae	2282:2296	Ruminococcaceae	2282:2296	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	12	88	from	decrease	2026:2033	arg1	Senegalimassilia					2051:2066	Senegalimassilia	2051:2066	Senegalimassilia	2051:2066	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	12	88	from	decrease	2026:2033	arg1	group					2216:2220	Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group	2151:2220	Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group	2151:2220	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	12	88	from	decrease	2026:2033	arg1	coprostanoligenes					2247:2263	Moryella, [Eubacterium] coprostanoligenes	2223:2263	coprostanoligenes	2247:2263	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	14	89	dep	CONCLUSIONS	2688:2698	arg1	shown					2729:2733	shown	2729:2733	shown	2729:2733	CONCLUSIONS In the present study we have shown, that Lythrum salicaria herb fulfills the criteria of a potential candidate for antidiarrheal agent, which could be applied as therapy or prevention of post-weaning diarrhea in piglets.
32673710	9	90	from	production	1503:1512	arg1	cultures					1525:1532	cultures	1525:1532	cultures	1525:1532	The production of SCFA in cultures was determined by GC analysis.
32673710	4	91	theme	Post-weaning	643:654	arg1	diarrhea					656:663	Post-weaning diarrhea	643:663	Post-weaning diarrhea	643:663	Post-weaning diarrhea has various causes, all of which have been linked to imbalances of intestinal microbiota.
32673710	13	92	theme	taxa	2627:2630	arg1	abundance					2632:2640	taxa abundance	2627:2640	taxa abundance	2627:2640	Significant correlations between taxa abundance and production of urolithins were determined.
32673710	5	93	from	production	967:976	arg1	constituents					1043:1054	the extract constituents	1031:1054	the extract constituents	1031:1054	The aim of the present study was to determine the interaction of LSH with the gut microbiota of healthy post-weaning piglets in order to evaluate its influence on microbiota composition and metabolism as well as production of potentially bioactive postbiotic metabolites from the extract constituents.
32673710	5	93	from	production	967:976	arg1	metabolism					945:954	metabolism	945:954	metabolism	945:954	The aim of the present study was to determine the interaction of LSH with the gut microbiota of healthy post-weaning piglets in order to evaluate its influence on microbiota composition and metabolism as well as production of potentially bioactive postbiotic metabolites from the extract constituents.
32673710	5	93	from	production	967:976	arg1	composition					929:939	microbiota composition	918:939	microbiota composition	918:939	The aim of the present study was to determine the interaction of LSH with the gut microbiota of healthy post-weaning piglets in order to evaluate its influence on microbiota composition and metabolism as well as production of potentially bioactive postbiotic metabolites from the extract constituents.
32673710	2	94	from	therapy	349:355	arg1	medicine					407:414	human and veterinary medicine	386:414	human and veterinary medicine	386:414	ETHNOPHARMACOLOGICAL RELEVANCE Herb of purple loosestrife (Lythrum salicaria L. from Lythraceae family) (LSH) was used in Europe since ancient times till early-20th century in the therapy of diarrhea and dysentery in human and veterinary medicine.
32673710	2	95	theme	loosestrife	215:225	arg1	Herb					200:203	ETHNOPHARMACOLOGICAL RELEVANCE Herb	169:203	ETHNOPHARMACOLOGICAL RELEVANCE Herb of purple loosestrife (Lythrum salicaria L. from Lythraceae family) (LSH)	169:277	ETHNOPHARMACOLOGICAL RELEVANCE Herb of purple loosestrife (Lythrum salicaria L. from Lythraceae family) (LSH) was used in Europe since ancient times till early-20th century in the therapy of diarrhea and dysentery in human and veterinary medicine.
32673710	15	96	theme	gut	2947:2949	arg1	composition					2962:2972	the gut microbiota composition	2943:2972	the gut microbiota composition	2943:2972	It not only modulates the gut microbiota composition without causing the dysbiosis and impairing metabolic activity, but is also a source of postbiotic metabolites, namely urolithins, which anti-inflammatory properties can be beneficial for gut health of piglets during the weaning period.
32673710	14	97	theme	Lythrum	2741:2747	arg1	herb					2759:2762	Lythrum salicaria herb	2741:2762	Lythrum salicaria herb	2741:2762	CONCLUSIONS In the present study we have shown, that Lythrum salicaria herb fulfills the criteria of a potential candidate for antidiarrheal agent, which could be applied as therapy or prevention of post-weaning diarrhea in piglets.
32673710	1	98	theme	metabolites	136:146	arg1	formation					112:120	formation	112:120	formation of postbiotic metabolites in ex vivo cultures	112:166	Focus on prebiotic properties and formation of postbiotic metabolites in ex vivo cultures.
32673710	1	98	theme	metabolites	136:146	arg1	properties					97:106	prebiotic properties	87:106	prebiotic properties	87:106	Focus on prebiotic properties and formation of postbiotic metabolites in ex vivo cultures.
32673710	7	99	theme	metabolites	1285:1295	arg1	production					1260:1269	The production	1256:1269	The production of postbiotic metabolites	1256:1295	The production of postbiotic metabolites was determined using UPLC-DAD-MSn method.
32673710	11	100	theme	ellagitannins	1874:1886	arg1	moiety					1851:1856	the flavogalloyl moiety	1834:1856	the flavogalloyl moiety of C-glucosylic ellagitannins	1834:1886	Urolithin M6, M7, urolithin C, A and iso-urolithin A were detected together with a previously not described metabolite originating from the flavogalloyl moiety of C-glucosylic ellagitannins.
32673710	3	101	theme	reduced	582:588	arg1	gain					604:607	reduced average daily gain	582:607	reduced average daily gain	582:607	Post-weaning diarrhea is a main problem affecting global piglet production, which leads to significant economic losses because of increased morbidity and mortality, reduced average daily gain, and high antibiotic consumption.
32673710	5	102	theme	LSH	820:822	arg1	interaction					805:815	the interaction	801:815	the interaction of LSH with the gut microbiota of healthy post-weaning piglets	801:878	The aim of the present study was to determine the interaction of LSH with the gut microbiota of healthy post-weaning piglets in order to evaluate its influence on microbiota composition and metabolism as well as production of potentially bioactive postbiotic metabolites from the extract constituents.
32673710	10	103	dep	RESULTS	1565:1571	arg1	able					1621:1624	able	1621:1624	able	1621:1624	RESULTS Only the caecal and distal colon microbiota was able to hydrolyze and metabolize ellagitannins present in LSH to urolithins.
32673710	10	103	dep	RESULTS	1565:1571	arg1	microbiota					1606:1615	Only the caecal and distal colon microbiota	1573:1615	Only the caecal and distal colon microbiota	1573:1615	RESULTS Only the caecal and distal colon microbiota was able to hydrolyze and metabolize ellagitannins present in LSH to urolithins.
32673710	9	104	theme	SCFA	1517:1520	arg1	production					1503:1512	The production	1499:1512	The production of SCFA in cultures	1499:1532	The production of SCFA in cultures was determined by GC analysis.
32673710	15	105	theme	piglets	3176:3182	arg1	health					3166:3171	gut health	3162:3171	gut health of piglets	3162:3182	It not only modulates the gut microbiota composition without causing the dysbiosis and impairing metabolic activity, but is also a source of postbiotic metabolites, namely urolithins, which anti-inflammatory properties can be beneficial for gut health of piglets during the weaning period.
32673710	6	106	theme	various	1211:1217	arg1	media					1227:1231	various culture media	1211:1231	various culture media supplemented with LSH	1211:1253	MATERIALS AND METHODS Ex vivo anaerobic cultures of piglets intestinal microbiota obtained from jejunum, ileum, caecum and distal colon were conducted in various culture media supplemented with LSH.
32673710	5	107	theme	extract	1035:1041	arg1	constituents					1043:1054	the extract constituents	1031:1054	the extract constituents	1031:1054	The aim of the present study was to determine the interaction of LSH with the gut microbiota of healthy post-weaning piglets in order to evaluate its influence on microbiota composition and metabolism as well as production of potentially bioactive postbiotic metabolites from the extract constituents.
32673710	12	108	theme	relative	2400:2407	arg1	abundance					2409:2417	the relative abundance	2396:2417	the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera	2396:2581	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	12	109	theme	RC9	2136:2138	arg1	group					2144:2148	Porphyromonadaceae, Rikenellaceae RC9 gut group	2102:2148	Porphyromonadaceae, Rikenellaceae RC9 gut group	2102:2148	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	14	110	theme	potential	2791:2799	arg1	prevention					2873:2882	prevention	2873:2882	prevention	2873:2882	CONCLUSIONS In the present study we have shown, that Lythrum salicaria herb fulfills the criteria of a potential candidate for antidiarrheal agent, which could be applied as therapy or prevention of post-weaning diarrhea in piglets.
32673710	14	110	theme	potential	2791:2799	arg1	therapy					2862:2868	therapy	2862:2868	therapy	2862:2868	CONCLUSIONS In the present study we have shown, that Lythrum salicaria herb fulfills the criteria of a potential candidate for antidiarrheal agent, which could be applied as therapy or prevention of post-weaning diarrhea in piglets.
32673710	14	110	theme	potential	2791:2799	arg1	candidate					2801:2809	a potential candidate	2789:2809	a potential candidate	2789:2809	CONCLUSIONS In the present study we have shown, that Lythrum salicaria herb fulfills the criteria of a potential candidate for antidiarrheal agent, which could be applied as therapy or prevention of post-weaning diarrhea in piglets.
32673710	5	111	from	constituents	1043:1054	arg1	influence					905:913	its influence	901:913	its influence on microbiota composition and metabolism as well as production of potentially bioactive postbiotic metabolites from the extract constituents	901:1054	The aim of the present study was to determine the interaction of LSH with the gut microbiota of healthy post-weaning piglets in order to evaluate its influence on microbiota composition and metabolism as well as production of potentially bioactive postbiotic metabolites from the extract constituents.
32673710	5	111	from	constituents	1043:1054	arg1	metabolites					1014:1024	potentially bioactive postbiotic metabolites	981:1024	potentially bioactive postbiotic metabolites from the extract constituents	981:1054	The aim of the present study was to determine the interaction of LSH with the gut microbiota of healthy post-weaning piglets in order to evaluate its influence on microbiota composition and metabolism as well as production of potentially bioactive postbiotic metabolites from the extract constituents.
32673710	5	111	from	constituents	1043:1054	arg1	production					967:976	production	967:976	its influence on microbiota composition and metabolism as well as production of potentially bioactive postbiotic metabolites from the extract constituents	901:1054	The aim of the present study was to determine the interaction of LSH with the gut microbiota of healthy post-weaning piglets in order to evaluate its influence on microbiota composition and metabolism as well as production of potentially bioactive postbiotic metabolites from the extract constituents.
32673710	3	112	theme	daily	598:602	arg1	gain					604:607	reduced average daily gain	582:607	reduced average daily gain	582:607	Post-weaning diarrhea is a main problem affecting global piglet production, which leads to significant economic losses because of increased morbidity and mortality, reduced average daily gain, and high antibiotic consumption.
32673710	5	113	theme	healthy	851:857	arg1	piglets					872:878	healthy post-weaning piglets	851:878	healthy post-weaning piglets	851:878	The aim of the present study was to determine the interaction of LSH with the gut microbiota of healthy post-weaning piglets in order to evaluate its influence on microbiota composition and metabolism as well as production of potentially bioactive postbiotic metabolites from the extract constituents.
32673710	12	114	theme	metabolic	1950:1958	arg1	activity					1960:1967	metabolic activity	1950:1967	metabolic activity	1950:1967	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	1	115	theme	ex	151:152	arg1	cultures					159:166	ex vivo cultures	151:166	ex vivo cultures	151:166	Focus on prebiotic properties and formation of postbiotic metabolites in ex vivo cultures.
32673710	8	116	theme	Bacterial	1339:1347	arg1	DNA					1357:1359	Bacterial genomic DNA	1339:1359	Bacterial genomic DNA	1339:1359	Bacterial genomic DNA was extracted and examined by sequencing by amplification of the 16S rDNA V3-V4 hypervariable regions followed by bioinformatic analysis.
32673710	12	117	theme	Lachnospiraceae	2192:2206	arg1	group					2216:2220	Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group	2151:2220	Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group	2151:2220	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	12	117	theme	Lachnospiraceae	2192:2206	arg1	Senegalimassilia					2051:2066	Senegalimassilia	2051:2066	Senegalimassilia	2051:2066	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	2	118	from	Europe	291:296	arg1	therapy					349:355	the therapy	345:355	the therapy of diarrhea and dysentery in human and veterinary medicine	345:414	ETHNOPHARMACOLOGICAL RELEVANCE Herb of purple loosestrife (Lythrum salicaria L. from Lythraceae family) (LSH) was used in Europe since ancient times till early-20th century in the therapy of diarrhea and dysentery in human and veterinary medicine.
32673710	4	119	theme	microbiota	743:752	arg1	imbalances					718:727	imbalances	718:727	imbalances of intestinal microbiota	718:752	Post-weaning diarrhea has various causes, all of which have been linked to imbalances of intestinal microbiota.
32673710	3	120	theme	global	467:472	arg1	production					481:490	global piglet production	467:490	global piglet production	467:490	Post-weaning diarrhea is a main problem affecting global piglet production, which leads to significant economic losses because of increased morbidity and mortality, reduced average daily gain, and high antibiotic consumption.
32673710	6	121	theme	Ex	1079:1080	arg1	cultures					1097:1104	Ex vivo anaerobic cultures	1079:1104	Ex vivo anaerobic cultures of piglets intestinal microbiota	1079:1137	MATERIALS AND METHODS Ex vivo anaerobic cultures of piglets intestinal microbiota obtained from jejunum, ileum, caecum and distal colon were conducted in various culture media supplemented with LSH.
32673710	11	122	theme	described	1796:1804	arg1	metabolite					1806:1815	a previously not described metabolite	1779:1815	a previously not described metabolite originating from the flavogalloyl moiety of C-glucosylic ellagitannins	1779:1886	Urolithin M6, M7, urolithin C, A and iso-urolithin A were detected together with a previously not described metabolite originating from the flavogalloyl moiety of C-glucosylic ellagitannins.
32673710	12	123	theme	torques	2521:2527	arg1	group					2469:2473	Prevotella, Agathobacter, [Eubacterium] hallii group	2422:2473	group	2469:2473	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	12	123	theme	torques	2521:2527	arg1	group					2529:2533	[Ruminococcus] torques group	2506:2533	[Ruminococcus] torques group	2506:2533	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	6	124	dep	MATERIALS	1057:1065	arg1	cultures					1097:1104	Ex vivo anaerobic cultures	1079:1104	Ex vivo anaerobic cultures of piglets intestinal microbiota	1079:1137	MATERIALS AND METHODS Ex vivo anaerobic cultures of piglets intestinal microbiota obtained from jejunum, ileum, caecum and distal colon were conducted in various culture media supplemented with LSH.
32673710	10	125	theme	present	1668:1674	arg1	ellagitannins					1654:1666	ellagitannins	1654:1666	ellagitannins present in LSH to urolithins	1654:1695	RESULTS Only the caecal and distal colon microbiota was able to hydrolyze and metabolize ellagitannins present in LSH to urolithins.
32673710	1	126	from	formation	112:120	arg1	cultures					159:166	ex vivo cultures	151:166	ex vivo cultures	151:166	Focus on prebiotic properties and formation of postbiotic metabolites in ex vivo cultures.
32673710	1	127	theme	prebiotic	87:95	arg1	properties					97:106	prebiotic properties	87:106	prebiotic properties	87:106	Focus on prebiotic properties and formation of postbiotic metabolites in ex vivo cultures.
32673710	2	128	theme	RELEVANCE	190:198	arg1	Herb					200:203	ETHNOPHARMACOLOGICAL RELEVANCE Herb	169:203	ETHNOPHARMACOLOGICAL RELEVANCE Herb of purple loosestrife (Lythrum salicaria L. from Lythraceae family) (LSH)	169:277	ETHNOPHARMACOLOGICAL RELEVANCE Herb of purple loosestrife (Lythrum salicaria L. from Lythraceae family) (LSH) was used in Europe since ancient times till early-20th century in the therapy of diarrhea and dysentery in human and veterinary medicine.
32673710	12	129	theme	significant	1900:1910	arg1	influence					1912:1920	no significant influence	1897:1920	no significant influence	1897:1920	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	5	130	theme	bioactive	993:1001	arg1	metabolites					1014:1024	potentially bioactive postbiotic metabolites	981:1024	potentially bioactive postbiotic metabolites from the extract constituents	981:1054	The aim of the present study was to determine the interaction of LSH with the gut microbiota of healthy post-weaning piglets in order to evaluate its influence on microbiota composition and metabolism as well as production of potentially bioactive postbiotic metabolites from the extract constituents.
32673710	15	131	theme	postbiotic	3062:3071	arg1	metabolites					3073:3083	postbiotic metabolites	3062:3083	postbiotic metabolites	3062:3083	It not only modulates the gut microbiota composition without causing the dysbiosis and impairing metabolic activity, but is also a source of postbiotic metabolites, namely urolithins, which anti-inflammatory properties can be beneficial for gut health of piglets during the weaning period.
32673710	15	131	theme	postbiotic	3062:3071	arg1	urolithins					3093:3102	urolithins	3093:3102	namely urolithins	3086:3102	It not only modulates the gut microbiota composition without causing the dysbiosis and impairing metabolic activity, but is also a source of postbiotic metabolites, namely urolithins, which anti-inflammatory properties can be beneficial for gut health of piglets during the weaning period.
32673710	3	132	theme	Post-weaning	417:428	arg1	diarrhea					430:437	Post-weaning diarrhea	417:437	Post-weaning diarrhea	417:437	Post-weaning diarrhea is a main problem affecting global piglet production, which leads to significant economic losses because of increased morbidity and mortality, reduced average daily gain, and high antibiotic consumption.
32673710	3	132	theme	Post-weaning	417:428	arg1	problem					449:455	a main problem	442:455	a main problem affecting global piglet production, which leads to significant economic losses because of increased morbidity and mortality, reduced average daily gain, and high antibiotic consumption	442:640	Post-weaning diarrhea is a main problem affecting global piglet production, which leads to significant economic losses because of increased morbidity and mortality, reduced average daily gain, and high antibiotic consumption.
32673710	14	133	dep	Lythrum	2741:2747	arg1	salicaria					2749:2757	salicaria	2749:2757	salicaria	2749:2757	CONCLUSIONS In the present study we have shown, that Lythrum salicaria herb fulfills the criteria of a potential candidate for antidiarrheal agent, which could be applied as therapy or prevention of post-weaning diarrhea in piglets.
32673710	3	134	theme	high	614:617	arg1	consumption					630:640	high antibiotic consumption	614:640	high antibiotic consumption	614:640	Post-weaning diarrhea is a main problem affecting global piglet production, which leads to significant economic losses because of increased morbidity and mortality, reduced average daily gain, and high antibiotic consumption.
32673710	12	135	theme	Moryella	2223:2230	arg1	Senegalimassilia					2051:2066	Senegalimassilia	2051:2066	Senegalimassilia	2051:2066	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	12	135	theme	Moryella	2223:2230	arg1	coprostanoligenes					2247:2263	Moryella, [Eubacterium] coprostanoligenes	2223:2263	coprostanoligenes	2247:2263	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	14	136	from	diarrhea	2900:2907	arg1	piglets					2912:2918	piglets	2912:2918	piglets	2912:2918	CONCLUSIONS In the present study we have shown, that Lythrum salicaria herb fulfills the criteria of a potential candidate for antidiarrheal agent, which could be applied as therapy or prevention of post-weaning diarrhea in piglets.
32673710	5	137	theme	present	770:776	arg1	study					778:782	the present study	766:782	the present study	766:782	The aim of the present study was to determine the interaction of LSH with the gut microbiota of healthy post-weaning piglets in order to evaluate its influence on microbiota composition and metabolism as well as production of potentially bioactive postbiotic metabolites from the extract constituents.
32673710	5	138	theme	metabolites	1014:1024	arg1	influence					905:913	its influence	901:913	its influence on microbiota composition and metabolism as well as production of potentially bioactive postbiotic metabolites from the extract constituents	901:1054	The aim of the present study was to determine the interaction of LSH with the gut microbiota of healthy post-weaning piglets in order to evaluate its influence on microbiota composition and metabolism as well as production of potentially bioactive postbiotic metabolites from the extract constituents.
32673710	5	138	theme	metabolites	1014:1024	arg1	production					967:976	production	967:976	its influence on microbiota composition and metabolism as well as production of potentially bioactive postbiotic metabolites from the extract constituents	901:1054	The aim of the present study was to determine the interaction of LSH with the gut microbiota of healthy post-weaning piglets in order to evaluate its influence on microbiota composition and metabolism as well as production of potentially bioactive postbiotic metabolites from the extract constituents.
32673710	10	139	from	present	1668:1674	arg1	LSH					1679:1681	LSH	1679:1681	LSH to urolithins	1679:1695	RESULTS Only the caecal and distal colon microbiota was able to hydrolyze and metabolize ellagitannins present in LSH to urolithins.
32673710	7	140	theme	postbiotic	1274:1283	arg1	metabolites					1285:1295	postbiotic metabolites	1274:1295	postbiotic metabolites	1274:1295	The production of postbiotic metabolites was determined using UPLC-DAD-MSn method.
32673710	12	141	theme	[Ruminococcus	2506:2518	arg1	torques					2521:2527	[Ruminococcus] torques	2506:2527	[Ruminococcus] torques group	2506:2533	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	4	142	contain	has	665:667	arg1	diarrhea					656:663	Post-weaning diarrhea	643:663	Post-weaning diarrhea	643:663	Post-weaning diarrhea has various causes, all of which have been linked to imbalances of intestinal microbiota.
32673710	4	142	contain	has	665:667	arg2	causes					677:682	various causes	669:682	various causes	669:682	Post-weaning diarrhea has various causes, all of which have been linked to imbalances of intestinal microbiota.
32673710	14	143	from	piglets	2912:2918	arg1	prevention					2873:2882	prevention	2873:2882	prevention	2873:2882	CONCLUSIONS In the present study we have shown, that Lythrum salicaria herb fulfills the criteria of a potential candidate for antidiarrheal agent, which could be applied as therapy or prevention of post-weaning diarrhea in piglets.
32673710	14	143	from	piglets	2912:2918	arg1	therapy					2862:2868	therapy	2862:2868	therapy	2862:2868	CONCLUSIONS In the present study we have shown, that Lythrum salicaria herb fulfills the criteria of a potential candidate for antidiarrheal agent, which could be applied as therapy or prevention of post-weaning diarrhea in piglets.
32673710	14	143	from	piglets	2912:2918	arg1	candidate					2801:2809	a potential candidate	2789:2809	a potential candidate	2789:2809	CONCLUSIONS In the present study we have shown, that Lythrum salicaria herb fulfills the criteria of a potential candidate for antidiarrheal agent, which could be applied as therapy or prevention of post-weaning diarrhea in piglets.
32673710	2	144	theme	till	318:321	arg1	century					334:340	ancient times till early-20th century	304:340	ancient times till early-20th century	304:340	ETHNOPHARMACOLOGICAL RELEVANCE Herb of purple loosestrife (Lythrum salicaria L. from Lythraceae family) (LSH) was used in Europe since ancient times till early-20th century in the therapy of diarrhea and dysentery in human and veterinary medicine.
32673710	0	145	dep	herb	21:24	arg1	microbiota					34:43	microbiota	34:43	microbiota	34:43	Lythrum salicaria L. herb and gut microbiota of healthy post-weaning piglets.
32673710	10	146	from	LSH	1679:1681	arg1	present					1668:1674	present	1668:1674	present	1668:1674	RESULTS Only the caecal and distal colon microbiota was able to hydrolyze and metabolize ellagitannins present in LSH to urolithins.
32673710	6	147	theme	intestinal	1117:1126	arg1	microbiota					1128:1137	piglets intestinal microbiota	1109:1137	piglets intestinal microbiota	1109:1137	MATERIALS AND METHODS Ex vivo anaerobic cultures of piglets intestinal microbiota obtained from jejunum, ileum, caecum and distal colon were conducted in various culture media supplemented with LSH.
32673710	12	148	theme	group	2469:2473	arg1	abundance					2409:2417	the relative abundance	2396:2417	the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera	2396:2581	LSH had no significant influence on microbiota diversity and metabolic activity, but was able to modulate its composition by significant decrease in Collinsella, Senegalimassilia, uncultured bacteria belonging to Porphyromonadaceae, Rikenellaceae RC9 gut group, Mogibacterium, Dorea, Lachnoclostridium, Lachnospiraceae UCG-004 group, Moryella, [Eubacterium] coprostanoligenes, Intestinimonas, Ruminococcaceae UCG-005, uncultured bacteria belonging to Ruminococcaceae, Acidaminococcus and Allisonella, while the relative abundance of Prevotella, Agathobacter, [Eubacterium] hallii group, Lachnospiraceae NK3A20 group, [Ruminococcus] torques group, Catenibacterium, Catenisphaera and Megasphaera increased.
32673710	8	149	theme	rDNA	1430:1433	arg1	regions					1455:1461	the 16S rDNA V3-V4 hypervariable regions	1422:1461	the 16S rDNA V3-V4 hypervariable regions followed by bioinformatic analysis	1422:1496	Bacterial genomic DNA was extracted and examined by sequencing by amplification of the 16S rDNA V3-V4 hypervariable regions followed by bioinformatic analysis.
32673710	2	150	theme	ancient	304:310	arg1	times					312:316	ancient times	304:316	ancient times till early-20th century	304:340	ETHNOPHARMACOLOGICAL RELEVANCE Herb of purple loosestrife (Lythrum salicaria L. from Lythraceae family) (LSH) was used in Europe since ancient times till early-20th century in the therapy of diarrhea and dysentery in human and veterinary medicine.
32673710	5	151	from	influence	905:913	arg1	constituents					1043:1054	the extract constituents	1031:1054	the extract constituents	1031:1054	The aim of the present study was to determine the interaction of LSH with the gut microbiota of healthy post-weaning piglets in order to evaluate its influence on microbiota composition and metabolism as well as production of potentially bioactive postbiotic metabolites from the extract constituents.
32673710	5	151	from	influence	905:913	arg1	metabolism					945:954	metabolism	945:954	metabolism	945:954	The aim of the present study was to determine the interaction of LSH with the gut microbiota of healthy post-weaning piglets in order to evaluate its influence on microbiota composition and metabolism as well as production of potentially bioactive postbiotic metabolites from the extract constituents.
32673710	5	151	from	influence	905:913	arg1	composition					929:939	microbiota composition	918:939	microbiota composition	918:939	The aim of the present study was to determine the interaction of LSH with the gut microbiota of healthy post-weaning piglets in order to evaluate its influence on microbiota composition and metabolism as well as production of potentially bioactive postbiotic metabolites from the extract constituents.
32673710	2	152	dep	loosestrife	215:225	arg1	Lythrum					228:234	Lythrum	228:234	Lythrum	228:234	ETHNOPHARMACOLOGICAL RELEVANCE Herb of purple loosestrife (Lythrum salicaria L. from Lythraceae family) (LSH) was used in Europe since ancient times till early-20th century in the therapy of diarrhea and dysentery in human and veterinary medicine.
32673710	8	153	theme	hypervariable	1441:1453	arg1	regions					1455:1461	the 16S rDNA V3-V4 hypervariable regions	1422:1461	the 16S rDNA V3-V4 hypervariable regions followed by bioinformatic analysis	1422:1496	Bacterial genomic DNA was extracted and examined by sequencing by amplification of the 16S rDNA V3-V4 hypervariable regions followed by bioinformatic analysis.
32673710	15	154	theme	weaning	3195:3201	arg1	period					3203:3208	the weaning period	3191:3208	the weaning period	3191:3208	It not only modulates the gut microbiota composition without causing the dysbiosis and impairing metabolic activity, but is also a source of postbiotic metabolites, namely urolithins, which anti-inflammatory properties can be beneficial for gut health of piglets during the weaning period.
32583466	2	0	dep	young	563:567	arg1	healthy					570:576	healthy	570:576	healthy	570:576	Here, we investigated the potential effects of a genome-wide psychiatric risk variant in the NCAN gene encoding the ECM proteoglycan neurocan (rs1064395) on memory performance, hippocampal function and cortical morphology in young, healthy volunteers.
32583466	4	1	theme	novelty-encoding	824:839	arg1	task					841:844	a novelty-encoding task	822:844	a novelty-encoding task with visual scenes	822:863	In 117 participants, we performed functional magnetic resonance imaging using a novelty-encoding task with visual scenes.
32583466	6	2	theme	voxel-based	1072:1082	arg1	morphometry					1084:1094	voxel-based morphometry	1072:1094	voxel-based morphometry	1072:1094	To assess effects of rs1064395 on brain morphology, we performed voxel-based morphometry in 420 participants from four independent cohorts and found lower grey matter density in the ventrolateral and rostral prefrontal cortex of risk allele carriers.
32583466	2	3	from	morphology	549:558	arg1	volunteers					578:587	young, healthy volunteers	563:587	young, healthy volunteers	563:587	Here, we investigated the potential effects of a genome-wide psychiatric risk variant in the NCAN gene encoding the ECM proteoglycan neurocan (rs1064395) on memory performance, hippocampal function and cortical morphology in young, healthy volunteers.
32583466	7	4	theme	complex	1449:1455	arg1	effect					1457:1462	a more complex effect	1442:1462	a more complex effect of the SNP on ECM composition	1442:1492	In silico eQTL analysis revealed that rs1064395 SNP is linked not only to increased prefrontal expression of the NCAN gene itself, but also of the neighbouring HAPLN4 gene, suggesting a more complex effect of the SNP on ECM composition.
32583466	7	5	theme	neighbouring	1405:1416	arg1	gene					1425:1428	the neighbouring HAPLN4 gene	1401:1428	the neighbouring HAPLN4 gene	1401:1428	In silico eQTL analysis revealed that rs1064395 SNP is linked not only to increased prefrontal expression of the NCAN gene itself, but also of the neighbouring HAPLN4 gene, suggesting a more complex effect of the SNP on ECM composition.
32583466	8	6	theme	variation	1612:1620	arg1	structure					1643:1651	variation of prefrontal cortex structure	1612:1651	variation of prefrontal cortex structure	1612:1651	Our results suggest that the NCAN rs1064395 A allele is associated with lower hippocampus-dependent memory function, variation of prefrontal cortex structure and ECM composition.
32583466	1	7	theme	key	259:261	arg1	region					263:268	a key region	257:268	a key region in human memory that is commonly affected in psychiatric disorders	257:335	Alterations of the brain extracellular matrix (ECM) can perturb the structure and function of brain networks like the hippocampus, a key region in human memory that is commonly affected in psychiatric disorders.
32583466	1	7	theme	key	259:261	arg1	hippocampus					244:254	the hippocampus	240:254	the hippocampus	240:254	Alterations of the brain extracellular matrix (ECM) can perturb the structure and function of brain networks like the hippocampus, a key region in human memory that is commonly affected in psychiatric disorders.
32583466	4	8	theme	resonance	798:806	arg1	imaging					808:814	functional magnetic resonance imaging	778:814	functional magnetic resonance imaging	778:814	In 117 participants, we performed functional magnetic resonance imaging using a novelty-encoding task with visual scenes.
32583466	6	9	theme	grey	1162:1165	arg1	density					1174:1180	lower grey matter density	1156:1180	lower grey matter density	1156:1180	To assess effects of rs1064395 on brain morphology, we performed voxel-based morphometry in 420 participants from four independent cohorts and found lower grey matter density in the ventrolateral and rostral prefrontal cortex of risk allele carriers.
32583466	3	10	theme	recall	676:681	arg1	performance					683:693	reduced recall performance	668:693	reduced recall performance	668:693	We assessed verbal memory performance in two cohorts (N = 572, 302) and found reduced recall performance in risk allele (A) carriers across both cohorts.
32583466	6	11	theme	carriers	1248:1255	arg1	cortex					1226:1231	the ventrolateral and rostral prefrontal cortex	1185:1231	the ventrolateral and rostral prefrontal cortex of risk allele carriers	1185:1255	To assess effects of rs1064395 on brain morphology, we performed voxel-based morphometry in 420 participants from four independent cohorts and found lower grey matter density in the ventrolateral and rostral prefrontal cortex of risk allele carriers.
32583466	9	12	theme	prefrontal	1722:1731	arg1	dysfunction					1733:1743	the well-documented hippocampal and prefrontal dysfunction	1686:1743	the well-documented hippocampal and prefrontal dysfunction in bipolar disorder and schizophrenia	1686:1781	Considering the well-documented hippocampal and prefrontal dysfunction in bipolar disorder and schizophrenia, our results may reflect an intermediate phenotype by which NCAN rs1064395 contributes to disease risk.
32583466	3	13	theme	A	711:711	arg1	carriers					714:721	risk allele (A) carriers	698:721	risk allele (A) carriers across both cohorts	698:741	We assessed verbal memory performance in two cohorts (N = 572, 302) and found reduced recall performance in risk allele (A) carriers across both cohorts.
32583466	6	14	theme	risk	1236:1239	arg1	carriers					1248:1255	risk allele carriers	1236:1255	risk allele carriers	1236:1255	To assess effects of rs1064395 on brain morphology, we performed voxel-based morphometry in 420 participants from four independent cohorts and found lower grey matter density in the ventrolateral and rostral prefrontal cortex of risk allele carriers.
32583466	3	15	theme	allele	703:708	arg1	carriers					714:721	risk allele (A) carriers	698:721	risk allele (A) carriers across both cohorts	698:741	We assessed verbal memory performance in two cohorts (N = 572, 302) and found reduced recall performance in risk allele (A) carriers across both cohorts.
32583466	2	16	theme	hippocampal	515:525	arg1	function					527:534	hippocampal function	515:534	hippocampal function	515:534	Here, we investigated the potential effects of a genome-wide psychiatric risk variant in the NCAN gene encoding the ECM proteoglycan neurocan (rs1064395) on memory performance, hippocampal function and cortical morphology in young, healthy volunteers.
32583466	7	17	theme	NCAN	1371:1374	arg1	gene					1376:1379	the NCAN gene itself	1367:1386	the NCAN gene itself	1367:1386	In silico eQTL analysis revealed that rs1064395 SNP is linked not only to increased prefrontal expression of the NCAN gene itself, but also of the neighbouring HAPLN4 gene, suggesting a more complex effect of the SNP on ECM composition.
32583466	7	18	from	effect	1457:1462	arg1	composition					1482:1492	ECM composition	1478:1492	ECM composition	1478:1492	In silico eQTL analysis revealed that rs1064395 SNP is linked not only to increased prefrontal expression of the NCAN gene itself, but also of the neighbouring HAPLN4 gene, suggesting a more complex effect of the SNP on ECM composition.
32583466	2	19	theme	young	563:567	arg1	volunteers					578:587	young, healthy volunteers	563:587	young, healthy volunteers	563:587	Here, we investigated the potential effects of a genome-wide psychiatric risk variant in the NCAN gene encoding the ECM proteoglycan neurocan (rs1064395) on memory performance, hippocampal function and cortical morphology in young, healthy volunteers.
32583466	0	20	from	performance	70:80	arg1	humans					118:123	healthy humans	110:123	healthy humans	110:123	Neurocan genome-wide psychiatric risk variant affects explicit memory performance and hippocampal function in healthy humans.
32583466	6	21	theme	prefrontal	1215:1224	arg1	cortex					1226:1231	the ventrolateral and rostral prefrontal cortex	1185:1231	the ventrolateral and rostral prefrontal cortex of risk allele carriers	1185:1255	To assess effects of rs1064395 on brain morphology, we performed voxel-based morphometry in 420 participants from four independent cohorts and found lower grey matter density in the ventrolateral and rostral prefrontal cortex of risk allele carriers.
32583466	7	22	theme	prefrontal	1342:1351	arg1	expression					1353:1362	increased prefrontal expression	1332:1362	increased prefrontal expression of the NCAN gene itself	1332:1386	In silico eQTL analysis revealed that rs1064395 SNP is linked not only to increased prefrontal expression of the NCAN gene itself, but also of the neighbouring HAPLN4 gene, suggesting a more complex effect of the SNP on ECM composition.
32583466	3	23	theme	memory	609:614	arg1	performance					616:626	verbal memory performance	602:626	verbal memory performance	602:626	We assessed verbal memory performance in two cohorts (N = 572, 302) and found reduced recall performance in risk allele (A) carriers across both cohorts.
32583466	5	24	theme	higher	894:899	arg1	rates					913:917	higher false alarm rates	894:917	higher false alarm rates	894:917	Risk allele carriers showed higher false alarm rates during recognition, accompanied by inefficiently increased left hippocampal activation.
32583466	9	25	theme	bipolar	1748:1754	arg1	disorder					1756:1763	bipolar disorder	1748:1763	bipolar disorder	1748:1763	Considering the well-documented hippocampal and prefrontal dysfunction in bipolar disorder and schizophrenia, our results may reflect an intermediate phenotype by which NCAN rs1064395 contributes to disease risk.
32583466	1	26	theme	matrix	165:170	arg1	Alterations					126:136	Alterations	126:136	Alterations of the brain extracellular matrix (ECM)	126:176	Alterations of the brain extracellular matrix (ECM) can perturb the structure and function of brain networks like the hippocampus, a key region in human memory that is commonly affected in psychiatric disorders.
32583466	2	27	from	variant	416:422	arg1	gene					436:439	the NCAN gene	427:439	the NCAN gene encoding the ECM proteoglycan neurocan	427:478	Here, we investigated the potential effects of a genome-wide psychiatric risk variant in the NCAN gene encoding the ECM proteoglycan neurocan (rs1064395) on memory performance, hippocampal function and cortical morphology in young, healthy volunteers.
32583466	0	28	theme	hippocampal	86:96	arg1	function					98:105	hippocampal function	86:105	hippocampal function	86:105	Neurocan genome-wide psychiatric risk variant affects explicit memory performance and hippocampal function in healthy humans.
32583466	5	29	theme	Risk	866:869	arg1	carriers					878:885	Risk allele carriers	866:885	Risk allele carriers	866:885	Risk allele carriers showed higher false alarm rates during recognition, accompanied by inefficiently increased left hippocampal activation.
32583466	7	30	theme	HAPLN4	1418:1423	arg1	gene					1425:1428	the neighbouring HAPLN4 gene	1401:1428	the neighbouring HAPLN4 gene	1401:1428	In silico eQTL analysis revealed that rs1064395 SNP is linked not only to increased prefrontal expression of the NCAN gene itself, but also of the neighbouring HAPLN4 gene, suggesting a more complex effect of the SNP on ECM composition.
32583466	1	31	theme	brain	145:149	arg1	matrix					165:170	the brain extracellular matrix	141:170	the brain extracellular matrix (ECM)	141:176	Alterations of the brain extracellular matrix (ECM) can perturb the structure and function of brain networks like the hippocampus, a key region in human memory that is commonly affected in psychiatric disorders.
32583466	1	31	theme	brain	145:149	arg1	ECM					173:175	ECM	173:175	ECM	173:175	Alterations of the brain extracellular matrix (ECM) can perturb the structure and function of brain networks like the hippocampus, a key region in human memory that is commonly affected in psychiatric disorders.
32583466	2	32	from	gene	436:439	arg1	effects					374:380	the potential effects	360:380	the potential effects of a genome-wide psychiatric risk variant in the NCAN gene encoding the ECM proteoglycan neurocan (rs1064395) on memory performance, hippocampal function and cortical morphology in young, healthy volunteers	360:587	Here, we investigated the potential effects of a genome-wide psychiatric risk variant in the NCAN gene encoding the ECM proteoglycan neurocan (rs1064395) on memory performance, hippocampal function and cortical morphology in young, healthy volunteers.
32583466	2	33	theme	NCAN	431:434	arg1	gene					436:439	the NCAN gene	427:439	the NCAN gene encoding the ECM proteoglycan neurocan	427:478	Here, we investigated the potential effects of a genome-wide psychiatric risk variant in the NCAN gene encoding the ECM proteoglycan neurocan (rs1064395) on memory performance, hippocampal function and cortical morphology in young, healthy volunteers.
32583466	8	34	theme	lower	1567:1571	arg1	function					1602:1609	lower hippocampus-dependent memory function	1567:1609	lower hippocampus-dependent memory function	1567:1609	Our results suggest that the NCAN rs1064395 A allele is associated with lower hippocampus-dependent memory function, variation of prefrontal cortex structure and ECM composition.
32583466	2	35	theme	potential	364:372	arg1	effects					374:380	the potential effects	360:380	the potential effects of a genome-wide psychiatric risk variant in the NCAN gene encoding the ECM proteoglycan neurocan (rs1064395) on memory performance, hippocampal function and cortical morphology in young, healthy volunteers	360:587	Here, we investigated the potential effects of a genome-wide psychiatric risk variant in the NCAN gene encoding the ECM proteoglycan neurocan (rs1064395) on memory performance, hippocampal function and cortical morphology in young, healthy volunteers.
32583466	0	36	theme	Neurocan	0:7	arg1	variant					38:44	Neurocan genome-wide psychiatric risk variant	0:44	Neurocan genome-wide psychiatric risk variant	0:44	Neurocan genome-wide psychiatric risk variant affects explicit memory performance and hippocampal function in healthy humans.
32583466	5	37	theme	alarm	907:911	arg1	rates					913:917	higher false alarm rates	894:917	higher false alarm rates	894:917	Risk allele carriers showed higher false alarm rates during recognition, accompanied by inefficiently increased left hippocampal activation.
32583466	6	38	theme	brain	1041:1045	arg1	morphology					1047:1056	brain morphology	1041:1056	brain morphology	1041:1056	To assess effects of rs1064395 on brain morphology, we performed voxel-based morphometry in 420 participants from four independent cohorts and found lower grey matter density in the ventrolateral and rostral prefrontal cortex of risk allele carriers.
32583466	9	39	theme	intermediate	1811:1822	arg1	phenotype					1824:1832	an intermediate phenotype	1808:1832	an intermediate phenotype by which NCAN rs1064395 contributes to disease risk	1808:1884	Considering the well-documented hippocampal and prefrontal dysfunction in bipolar disorder and schizophrenia, our results may reflect an intermediate phenotype by which NCAN rs1064395 contributes to disease risk.
32583466	2	40	theme	risk	411:414	arg1	variant					416:422	a genome-wide psychiatric risk variant	385:422	a genome-wide psychiatric risk variant in the NCAN gene encoding the ECM proteoglycan neurocan (rs1064395)	385:490	Here, we investigated the potential effects of a genome-wide psychiatric risk variant in the NCAN gene encoding the ECM proteoglycan neurocan (rs1064395) on memory performance, hippocampal function and cortical morphology in young, healthy volunteers.
32583466	0	41	theme	psychiatric	21:31	arg1	variant					38:44	Neurocan genome-wide psychiatric risk variant	0:44	Neurocan genome-wide psychiatric risk variant	0:44	Neurocan genome-wide psychiatric risk variant affects explicit memory performance and hippocampal function in healthy humans.
32583466	1	42	theme	human	273:277	arg1	memory					279:284	human memory	273:284	human memory	273:284	Alterations of the brain extracellular matrix (ECM) can perturb the structure and function of brain networks like the hippocampus, a key region in human memory that is commonly affected in psychiatric disorders.
32583466	6	43	theme	rs1064395	1028:1036	arg1	effects					1017:1023	effects	1017:1023	effects of rs1064395 on brain morphology	1017:1056	To assess effects of rs1064395 on brain morphology, we performed voxel-based morphometry in 420 participants from four independent cohorts and found lower grey matter density in the ventrolateral and rostral prefrontal cortex of risk allele carriers.
32583466	2	44	theme	ECM	454:456	arg1	neurocan					471:478	the ECM proteoglycan neurocan	450:478	the ECM proteoglycan neurocan	450:478	Here, we investigated the potential effects of a genome-wide psychiatric risk variant in the NCAN gene encoding the ECM proteoglycan neurocan (rs1064395) on memory performance, hippocampal function and cortical morphology in young, healthy volunteers.
32583466	2	45	theme	genome-wide	387:397	arg1	variant					416:422	a genome-wide psychiatric risk variant	385:422	a genome-wide psychiatric risk variant in the NCAN gene encoding the ECM proteoglycan neurocan (rs1064395)	385:490	Here, we investigated the potential effects of a genome-wide psychiatric risk variant in the NCAN gene encoding the ECM proteoglycan neurocan (rs1064395) on memory performance, hippocampal function and cortical morphology in young, healthy volunteers.
32583466	4	46	theme	functional	778:787	arg1	resonance					798:806	functional magnetic resonance	778:806	functional magnetic resonance imaging	778:814	In 117 participants, we performed functional magnetic resonance imaging using a novelty-encoding task with visual scenes.
32583466	0	47	theme	explicit	54:61	arg1	performance					70:80	explicit memory performance	54:80	explicit memory performance	54:80	Neurocan genome-wide psychiatric risk variant affects explicit memory performance and hippocampal function in healthy humans.
32583466	1	48	theme	brain	220:224	arg1	networks					226:233	brain networks	220:233	brain networks	220:233	Alterations of the brain extracellular matrix (ECM) can perturb the structure and function of brain networks like the hippocampus, a key region in human memory that is commonly affected in psychiatric disorders.
32583466	5	49	theme	increased	968:976	arg1	activation					995:1004	inefficiently increased left hippocampal activation	954:1004	inefficiently increased left hippocampal activation	954:1004	Risk allele carriers showed higher false alarm rates during recognition, accompanied by inefficiently increased left hippocampal activation.
32583466	9	50	theme	disease	1873:1879	arg1	risk					1881:1884	disease risk	1873:1884	disease risk	1873:1884	Considering the well-documented hippocampal and prefrontal dysfunction in bipolar disorder and schizophrenia, our results may reflect an intermediate phenotype by which NCAN rs1064395 contributes to disease risk.
32583466	1	51	dep	structure	194:202	arg1	the					190:192	the	190:192	the	190:192	Alterations of the brain extracellular matrix (ECM) can perturb the structure and function of brain networks like the hippocampus, a key region in human memory that is commonly affected in psychiatric disorders.
32583466	8	52	theme	cortex	1636:1641	arg1	variation					1612:1620	variation	1612:1620	variation of prefrontal cortex structure	1612:1651	Our results suggest that the NCAN rs1064395 A allele is associated with lower hippocampus-dependent memory function, variation of prefrontal cortex structure and ECM composition.
32583466	5	53	theme	hippocampal	983:993	arg1	activation					995:1004	inefficiently increased left hippocampal activation	954:1004	inefficiently increased left hippocampal activation	954:1004	Risk allele carriers showed higher false alarm rates during recognition, accompanied by inefficiently increased left hippocampal activation.
32583466	2	54	from	function	527:534	arg1	volunteers					578:587	young, healthy volunteers	563:587	young, healthy volunteers	563:587	Here, we investigated the potential effects of a genome-wide psychiatric risk variant in the NCAN gene encoding the ECM proteoglycan neurocan (rs1064395) on memory performance, hippocampal function and cortical morphology in young, healthy volunteers.
32583466	3	55	theme	reduced	668:674	arg1	performance					683:693	reduced recall performance	668:693	reduced recall performance	668:693	We assessed verbal memory performance in two cohorts (N = 572, 302) and found reduced recall performance in risk allele (A) carriers across both cohorts.
32583466	1	56	from	region	263:268	arg1	memory					279:284	human memory	273:284	human memory	273:284	Alterations of the brain extracellular matrix (ECM) can perturb the structure and function of brain networks like the hippocampus, a key region in human memory that is commonly affected in psychiatric disorders.
32583466	8	57	theme	memory	1595:1600	arg1	function					1602:1609	lower hippocampus-dependent memory function	1567:1609	lower hippocampus-dependent memory function	1567:1609	Our results suggest that the NCAN rs1064395 A allele is associated with lower hippocampus-dependent memory function, variation of prefrontal cortex structure and ECM composition.
32583466	6	58	theme	matter	1167:1172	arg1	density					1174:1180	lower grey matter density	1156:1180	lower grey matter density	1156:1180	To assess effects of rs1064395 on brain morphology, we performed voxel-based morphometry in 420 participants from four independent cohorts and found lower grey matter density in the ventrolateral and rostral prefrontal cortex of risk allele carriers.
32583466	3	59	theme	risk	698:701	arg1	carriers					714:721	risk allele (A) carriers	698:721	risk allele (A) carriers across both cohorts	698:741	We assessed verbal memory performance in two cohorts (N = 572, 302) and found reduced recall performance in risk allele (A) carriers across both cohorts.
32583466	4	60	theme	magnetic	789:796	arg1	resonance					798:806	functional magnetic resonance	778:806	functional magnetic resonance imaging	778:814	In 117 participants, we performed functional magnetic resonance imaging using a novelty-encoding task with visual scenes.
32583466	6	61	theme	lower	1156:1160	arg1	density					1174:1180	lower grey matter density	1156:1180	lower grey matter density	1156:1180	To assess effects of rs1064395 on brain morphology, we performed voxel-based morphometry in 420 participants from four independent cohorts and found lower grey matter density in the ventrolateral and rostral prefrontal cortex of risk allele carriers.
32583466	6	62	theme	allele	1241:1246	arg1	carriers					1248:1255	risk allele carriers	1236:1255	risk allele carriers	1236:1255	To assess effects of rs1064395 on brain morphology, we performed voxel-based morphometry in 420 participants from four independent cohorts and found lower grey matter density in the ventrolateral and rostral prefrontal cortex of risk allele carriers.
32583466	6	63	from	effects	1017:1023	arg1	morphology					1047:1056	brain morphology	1041:1056	brain morphology	1041:1056	To assess effects of rs1064395 on brain morphology, we performed voxel-based morphometry in 420 participants from four independent cohorts and found lower grey matter density in the ventrolateral and rostral prefrontal cortex of risk allele carriers.
32583466	0	64	from	function	98:105	arg1	humans					118:123	healthy humans	110:123	healthy humans	110:123	Neurocan genome-wide psychiatric risk variant affects explicit memory performance and hippocampal function in healthy humans.
32583466	6	65	theme	independent	1126:1136	arg1	cohorts					1138:1144	four independent cohorts	1121:1144	four independent cohorts	1121:1144	To assess effects of rs1064395 on brain morphology, we performed voxel-based morphometry in 420 participants from four independent cohorts and found lower grey matter density in the ventrolateral and rostral prefrontal cortex of risk allele carriers.
32583466	9	66	theme	hippocampal	1706:1716	arg1	dysfunction					1733:1743	the well-documented hippocampal and prefrontal dysfunction	1686:1743	the well-documented hippocampal and prefrontal dysfunction in bipolar disorder and schizophrenia	1686:1781	Considering the well-documented hippocampal and prefrontal dysfunction in bipolar disorder and schizophrenia, our results may reflect an intermediate phenotype by which NCAN rs1064395 contributes to disease risk.
32583466	7	67	theme	gene	1376:1379	arg1	expression					1353:1362	increased prefrontal expression	1332:1362	increased prefrontal expression of the NCAN gene itself	1332:1386	In silico eQTL analysis revealed that rs1064395 SNP is linked not only to increased prefrontal expression of the NCAN gene itself, but also of the neighbouring HAPLN4 gene, suggesting a more complex effect of the SNP on ECM composition.
32583466	5	68	theme	allele	871:876	arg1	carriers					878:885	Risk allele carriers	866:885	Risk allele carriers	866:885	Risk allele carriers showed higher false alarm rates during recognition, accompanied by inefficiently increased left hippocampal activation.
32583466	4	69	theme	visual	851:856	arg1	scenes					858:863	visual scenes	851:863	visual scenes	851:863	In 117 participants, we performed functional magnetic resonance imaging using a novelty-encoding task with visual scenes.
32583466	6	70	theme	rostral	1207:1213	arg1	cortex					1226:1231	the ventrolateral and rostral prefrontal cortex	1185:1231	the ventrolateral and rostral prefrontal cortex of risk allele carriers	1185:1255	To assess effects of rs1064395 on brain morphology, we performed voxel-based morphometry in 420 participants from four independent cohorts and found lower grey matter density in the ventrolateral and rostral prefrontal cortex of risk allele carriers.
32583466	3	71	theme	verbal	602:607	arg1	performance					616:626	verbal memory performance	602:626	verbal memory performance	602:626	We assessed verbal memory performance in two cohorts (N = 572, 302) and found reduced recall performance in risk allele (A) carriers across both cohorts.
32583466	4	72	with	task	841:844	arg1	scenes					858:863	visual scenes	851:863	visual scenes	851:863	In 117 participants, we performed functional magnetic resonance imaging using a novelty-encoding task with visual scenes.
32583466	9	73	from	dysfunction	1733:1743	arg1	schizophrenia					1769:1781	schizophrenia	1769:1781	schizophrenia	1769:1781	Considering the well-documented hippocampal and prefrontal dysfunction in bipolar disorder and schizophrenia, our results may reflect an intermediate phenotype by which NCAN rs1064395 contributes to disease risk.
32583466	9	73	from	dysfunction	1733:1743	arg1	disorder					1756:1763	bipolar disorder	1748:1763	bipolar disorder	1748:1763	Considering the well-documented hippocampal and prefrontal dysfunction in bipolar disorder and schizophrenia, our results may reflect an intermediate phenotype by which NCAN rs1064395 contributes to disease risk.
32583466	2	74	theme	cortical	540:547	arg1	morphology					549:558	cortical morphology	540:558	cortical morphology	540:558	Here, we investigated the potential effects of a genome-wide psychiatric risk variant in the NCAN gene encoding the ECM proteoglycan neurocan (rs1064395) on memory performance, hippocampal function and cortical morphology in young, healthy volunteers.
32583466	2	75	from	performance	502:512	arg1	volunteers					578:587	young, healthy volunteers	563:587	young, healthy volunteers	563:587	Here, we investigated the potential effects of a genome-wide psychiatric risk variant in the NCAN gene encoding the ECM proteoglycan neurocan (rs1064395) on memory performance, hippocampal function and cortical morphology in young, healthy volunteers.
32583466	6	76	theme	ventrolateral	1189:1201	arg1	cortex					1226:1231	the ventrolateral and rostral prefrontal cortex	1185:1231	the ventrolateral and rostral prefrontal cortex of risk allele carriers	1185:1255	To assess effects of rs1064395 on brain morphology, we performed voxel-based morphometry in 420 participants from four independent cohorts and found lower grey matter density in the ventrolateral and rostral prefrontal cortex of risk allele carriers.
32583466	1	77	theme	extracellular	151:163	arg1	matrix					165:170	the brain extracellular matrix	141:170	the brain extracellular matrix (ECM)	141:176	Alterations of the brain extracellular matrix (ECM) can perturb the structure and function of brain networks like the hippocampus, a key region in human memory that is commonly affected in psychiatric disorders.
32583466	1	77	theme	extracellular	151:163	arg1	ECM					173:175	ECM	173:175	ECM	173:175	Alterations of the brain extracellular matrix (ECM) can perturb the structure and function of brain networks like the hippocampus, a key region in human memory that is commonly affected in psychiatric disorders.
32583466	7	78	theme	increased	1332:1340	arg1	expression					1353:1362	increased prefrontal expression	1332:1362	increased prefrontal expression of the NCAN gene itself	1332:1386	In silico eQTL analysis revealed that rs1064395 SNP is linked not only to increased prefrontal expression of the NCAN gene itself, but also of the neighbouring HAPLN4 gene, suggesting a more complex effect of the SNP on ECM composition.
32583466	1	79	theme	psychiatric	315:325	arg1	disorders					327:335	psychiatric disorders	315:335	psychiatric disorders	315:335	Alterations of the brain extracellular matrix (ECM) can perturb the structure and function of brain networks like the hippocampus, a key region in human memory that is commonly affected in psychiatric disorders.
32583466	2	80	theme	proteoglycan	458:469	arg1	neurocan					471:478	the ECM proteoglycan neurocan	450:478	the ECM proteoglycan neurocan	450:478	Here, we investigated the potential effects of a genome-wide psychiatric risk variant in the NCAN gene encoding the ECM proteoglycan neurocan (rs1064395) on memory performance, hippocampal function and cortical morphology in young, healthy volunteers.
32583466	0	81	theme	healthy	110:116	arg1	humans					118:123	healthy humans	110:123	healthy humans	110:123	Neurocan genome-wide psychiatric risk variant affects explicit memory performance and hippocampal function in healthy humans.
32583466	7	82	dep	In	1258:1259	arg1	silico					1261:1266	silico	1261:1266	silico	1261:1266	In silico eQTL analysis revealed that rs1064395 SNP is linked not only to increased prefrontal expression of the NCAN gene itself, but also of the neighbouring HAPLN4 gene, suggesting a more complex effect of the SNP on ECM composition.
32583466	2	83	theme	memory	495:500	arg1	performance					502:512	memory performance	495:512	memory performance	495:512	Here, we investigated the potential effects of a genome-wide psychiatric risk variant in the NCAN gene encoding the ECM proteoglycan neurocan (rs1064395) on memory performance, hippocampal function and cortical morphology in young, healthy volunteers.
32583466	0	84	theme	genome-wide	9:19	arg1	variant					38:44	Neurocan genome-wide psychiatric risk variant	0:44	Neurocan genome-wide psychiatric risk variant	0:44	Neurocan genome-wide psychiatric risk variant affects explicit memory performance and hippocampal function in healthy humans.
32583466	5	85	theme	false	901:905	arg1	rates					913:917	higher false alarm rates	894:917	higher false alarm rates	894:917	Risk allele carriers showed higher false alarm rates during recognition, accompanied by inefficiently increased left hippocampal activation.
32583466	9	86	theme	well-documented	1690:1704	arg1	dysfunction					1733:1743	the well-documented hippocampal and prefrontal dysfunction	1686:1743	the well-documented hippocampal and prefrontal dysfunction in bipolar disorder and schizophrenia	1686:1781	Considering the well-documented hippocampal and prefrontal dysfunction in bipolar disorder and schizophrenia, our results may reflect an intermediate phenotype by which NCAN rs1064395 contributes to disease risk.
32583466	6	87	from	cohorts	1138:1144	arg1	participants					1103:1114	420 participants	1099:1114	420 participants from four independent cohorts	1099:1144	To assess effects of rs1064395 on brain morphology, we performed voxel-based morphometry in 420 participants from four independent cohorts and found lower grey matter density in the ventrolateral and rostral prefrontal cortex of risk allele carriers.
32583466	0	88	theme	risk	33:36	arg1	variant					38:44	Neurocan genome-wide psychiatric risk variant	0:44	Neurocan genome-wide psychiatric risk variant	0:44	Neurocan genome-wide psychiatric risk variant affects explicit memory performance and hippocampal function in healthy humans.
32583466	7	89	theme	rs1064395	1296:1304	arg1	SNP					1306:1308	rs1064395 SNP	1296:1308	rs1064395 SNP	1296:1308	In silico eQTL analysis revealed that rs1064395 SNP is linked not only to increased prefrontal expression of the NCAN gene itself, but also of the neighbouring HAPLN4 gene, suggesting a more complex effect of the SNP on ECM composition.
32583466	2	90	theme	variant	416:422	arg1	effects					374:380	the potential effects	360:380	the potential effects of a genome-wide psychiatric risk variant in the NCAN gene encoding the ECM proteoglycan neurocan (rs1064395) on memory performance, hippocampal function and cortical morphology in young, healthy volunteers	360:587	Here, we investigated the potential effects of a genome-wide psychiatric risk variant in the NCAN gene encoding the ECM proteoglycan neurocan (rs1064395) on memory performance, hippocampal function and cortical morphology in young, healthy volunteers.
32583466	8	91	theme	NCAN	1524:1527	arg1	allele					1541:1546	the NCAN rs1064395 A allele	1520:1546	the NCAN rs1064395 A allele	1520:1546	Our results suggest that the NCAN rs1064395 A allele is associated with lower hippocampus-dependent memory function, variation of prefrontal cortex structure and ECM composition.
32583466	2	92	theme	psychiatric	399:409	arg1	variant					416:422	a genome-wide psychiatric risk variant	385:422	a genome-wide psychiatric risk variant in the NCAN gene encoding the ECM proteoglycan neurocan (rs1064395)	385:490	Here, we investigated the potential effects of a genome-wide psychiatric risk variant in the NCAN gene encoding the ECM proteoglycan neurocan (rs1064395) on memory performance, hippocampal function and cortical morphology in young, healthy volunteers.
32583466	8	93	theme	A	1539:1539	arg1	allele					1541:1546	the NCAN rs1064395 A allele	1520:1546	the NCAN rs1064395 A allele	1520:1546	Our results suggest that the NCAN rs1064395 A allele is associated with lower hippocampus-dependent memory function, variation of prefrontal cortex structure and ECM composition.
32583466	0	94	theme	memory	63:68	arg1	performance					70:80	explicit memory performance	54:80	explicit memory performance	54:80	Neurocan genome-wide psychiatric risk variant affects explicit memory performance and hippocampal function in healthy humans.
32583466	7	95	theme	eQTL	1268:1271	arg1	analysis					1273:1280	In silico eQTL analysis	1258:1280	In silico eQTL analysis	1258:1280	In silico eQTL analysis revealed that rs1064395 SNP is linked not only to increased prefrontal expression of the NCAN gene itself, but also of the neighbouring HAPLN4 gene, suggesting a more complex effect of the SNP on ECM composition.
32583466	8	96	theme	prefrontal	1625:1634	arg1	cortex					1636:1641	prefrontal cortex	1625:1641	prefrontal cortex	1625:1641	Our results suggest that the NCAN rs1064395 A allele is associated with lower hippocampus-dependent memory function, variation of prefrontal cortex structure and ECM composition.
32583466	7	97	theme	ECM	1478:1480	arg1	composition					1482:1492	ECM composition	1478:1492	ECM composition	1478:1492	In silico eQTL analysis revealed that rs1064395 SNP is linked not only to increased prefrontal expression of the NCAN gene itself, but also of the neighbouring HAPLN4 gene, suggesting a more complex effect of the SNP on ECM composition.
32583466	2	98	from	effects	374:380	arg1	function					527:534	hippocampal function	515:534	hippocampal function	515:534	Here, we investigated the potential effects of a genome-wide psychiatric risk variant in the NCAN gene encoding the ECM proteoglycan neurocan (rs1064395) on memory performance, hippocampal function and cortical morphology in young, healthy volunteers.
32583466	2	98	from	effects	374:380	arg1	morphology					549:558	cortical morphology	540:558	cortical morphology	540:558	Here, we investigated the potential effects of a genome-wide psychiatric risk variant in the NCAN gene encoding the ECM proteoglycan neurocan (rs1064395) on memory performance, hippocampal function and cortical morphology in young, healthy volunteers.
32583466	2	98	from	effects	374:380	arg1	gene					436:439	the NCAN gene	427:439	the NCAN gene encoding the ECM proteoglycan neurocan	427:478	Here, we investigated the potential effects of a genome-wide psychiatric risk variant in the NCAN gene encoding the ECM proteoglycan neurocan (rs1064395) on memory performance, hippocampal function and cortical morphology in young, healthy volunteers.
32583466	2	98	from	effects	374:380	arg1	performance					502:512	memory performance	495:512	memory performance	495:512	Here, we investigated the potential effects of a genome-wide psychiatric risk variant in the NCAN gene encoding the ECM proteoglycan neurocan (rs1064395) on memory performance, hippocampal function and cortical morphology in young, healthy volunteers.
32583466	7	99	theme	In	1258:1259	arg1	analysis					1273:1280	In silico eQTL analysis	1258:1280	In silico eQTL analysis	1258:1280	In silico eQTL analysis revealed that rs1064395 SNP is linked not only to increased prefrontal expression of the NCAN gene itself, but also of the neighbouring HAPLN4 gene, suggesting a more complex effect of the SNP on ECM composition.
32583466	7	100	attach	linked	1313:1318	arg1	expression					1353:1362	increased prefrontal expression	1332:1362	increased prefrontal expression of the NCAN gene itself	1332:1386	In silico eQTL analysis revealed that rs1064395 SNP is linked not only to increased prefrontal expression of the NCAN gene itself, but also of the neighbouring HAPLN4 gene, suggesting a more complex effect of the SNP on ECM composition.
32583466	7	100	attach	linked	1313:1318	arg2	SNP					1306:1308	rs1064395 SNP	1296:1308	rs1064395 SNP	1296:1308	In silico eQTL analysis revealed that rs1064395 SNP is linked not only to increased prefrontal expression of the NCAN gene itself, but also of the neighbouring HAPLN4 gene, suggesting a more complex effect of the SNP on ECM composition.
32583466	1	101	theme	networks	226:233	arg1	function					208:215	function	208:215	function	208:215	Alterations of the brain extracellular matrix (ECM) can perturb the structure and function of brain networks like the hippocampus, a key region in human memory that is commonly affected in psychiatric disorders.
32583466	1	101	theme	networks	226:233	arg1	structure					194:202	structure	194:202	structure	194:202	Alterations of the brain extracellular matrix (ECM) can perturb the structure and function of brain networks like the hippocampus, a key region in human memory that is commonly affected in psychiatric disorders.
32583466	7	102	theme	SNP	1471:1473	arg1	effect					1457:1462	a more complex effect	1442:1462	a more complex effect of the SNP on ECM composition	1442:1492	In silico eQTL analysis revealed that rs1064395 SNP is linked not only to increased prefrontal expression of the NCAN gene itself, but also of the neighbouring HAPLN4 gene, suggesting a more complex effect of the SNP on ECM composition.
32583466	5	103	theme	left	978:981	arg1	activation					995:1004	inefficiently increased left hippocampal activation	954:1004	inefficiently increased left hippocampal activation	954:1004	Risk allele carriers showed higher false alarm rates during recognition, accompanied by inefficiently increased left hippocampal activation.
32583466	8	104	theme	hippocampus-dependent	1573:1593	arg1	function					1602:1609	lower hippocampus-dependent memory function	1567:1609	lower hippocampus-dependent memory function	1567:1609	Our results suggest that the NCAN rs1064395 A allele is associated with lower hippocampus-dependent memory function, variation of prefrontal cortex structure and ECM composition.
32583466	4	105	theme	117	747:749	arg1	participants					751:762	117 participants	747:762	117 participants	747:762	In 117 participants, we performed functional magnetic resonance imaging using a novelty-encoding task with visual scenes.
32583466	8	106	theme	ECM	1657:1659	arg1	composition					1661:1671	ECM composition	1657:1671	ECM composition	1657:1671	Our results suggest that the NCAN rs1064395 A allele is associated with lower hippocampus-dependent memory function, variation of prefrontal cortex structure and ECM composition.
32583466	3	107	dep	cohorts	635:641	arg1	N = 572					644:650	N = 572	644:650	N = 572	644:650	We assessed verbal memory performance in two cohorts (N = 572, 302) and found reduced recall performance in risk allele (A) carriers across both cohorts.
32603032	7	0	theme	drug	984:987	arg1	drug					984:987	drug	984:987	drug	984:987	On the contrary, there is only a small amount of drug released in the absence of the enzyme.
32603032	7	0	theme	drug	984:987	arg1	amount					974:979	only a small amount	961:979	only a small amount of drug released in the absence of the enzyme	961:1025	On the contrary, there is only a small amount of drug released in the absence of the enzyme.
32603032	9	1	theme	RAW264.7	1320:1327	arg1	cells					1340:1344	RAW264.7 macrophage cells	1320:1344	RAW264.7 macrophage cells	1320:1344	Furthermore, the in vitro studies at the cellular level indicate that the enzyme-responsive prodrug can efficiently relieve inflammatory responses induced by lipopolysaccharide in RAW264.7 macrophage cells while indicating no cytotoxicity.
32603032	4	2	theme	acrylamide	609:618	arg1	groups					580:585	hydroxyl groups	571:585	hydroxyl groups of N-(2-hydroxyethyl) acrylamide	571:618	A monomer is first prepared after the esterification of carboxyl groups of indomethacin by hydroxyl groups of N-(2-hydroxyethyl) acrylamide.
32603032	4	2	theme	acrylamide	609:618	arg1	acrylamide					609:618	N-(2-hydroxyethyl) acrylamide	590:618	N-(2-hydroxyethyl) acrylamide	590:618	A monomer is first prepared after the esterification of carboxyl groups of indomethacin by hydroxyl groups of N-(2-hydroxyethyl) acrylamide.
32603032	5	3	theme	ester	679:683	arg1	bonds					685:689	ester bonds	679:689	ester bonds on the side chain	679:707	Then, a polymer prodrug, with indomethacin linked through ester bonds on the side chain, is synthesized by free radical polymerization of the monomer.
32603032	7	4	theme	small	968:972	arg1	drug					984:987	drug	984:987	drug	984:987	On the contrary, there is only a small amount of drug released in the absence of the enzyme.
32603032	7	4	theme	small	968:972	arg1	amount					974:979	only a small amount	961:979	only a small amount of drug released in the absence of the enzyme	961:1025	On the contrary, there is only a small amount of drug released in the absence of the enzyme.
32603032	3	5	theme	anti-inflammatory	372:388	arg1	prodrug					390:396	an enzyme-responsive anti-inflammatory prodrug	351:396	an enzyme-responsive anti-inflammatory prodrug capable of generating indomethacin upon the trigger of inflammation	351:464	In this work, an enzyme-responsive anti-inflammatory prodrug capable of generating indomethacin upon the trigger of inflammation is developed.
32603032	4	6	theme	groups	545:550	arg1	esterification					518:531	the esterification	514:531	the esterification of carboxyl groups of indomethacin	514:566	A monomer is first prepared after the esterification of carboxyl groups of indomethacin by hydroxyl groups of N-(2-hydroxyethyl) acrylamide.
32603032	2	7	theme	side	313:316	arg1	effects					318:324	the side effects	309:324	the side effects of NSAIDs	309:334	It is of great importance to configure new dosing strategies for alleviating the side effects of NSAIDs.
32603032	9	8	theme	in	1157:1158	arg1	studies					1166:1172	the in vitro studies	1153:1172	the in vitro studies at the cellular level	1153:1194	Furthermore, the in vitro studies at the cellular level indicate that the enzyme-responsive prodrug can efficiently relieve inflammatory responses induced by lipopolysaccharide in RAW264.7 macrophage cells while indicating no cytotoxicity.
32603032	6	9	theme	inflammation	909:920	arg1	environment					922:932	the inflammation environment	905:932	the inflammation environment	905:932	The therapeutic drug component can be triggered to release from the prodrug under the stimulation of cholesterol esterase, mimicking the inflammation environment.
32603032	5	10	theme	polymer	629:635	arg1	prodrug					637:643	a polymer prodrug	627:643	a polymer prodrug	627:643	Then, a polymer prodrug, with indomethacin linked through ester bonds on the side chain, is synthesized by free radical polymerization of the monomer.
32603032	9	11	theme	macrophage	1329:1338	arg1	cells					1340:1344	RAW264.7 macrophage cells	1320:1344	RAW264.7 macrophage cells	1320:1344	Furthermore, the in vitro studies at the cellular level indicate that the enzyme-responsive prodrug can efficiently relieve inflammatory responses induced by lipopolysaccharide in RAW264.7 macrophage cells while indicating no cytotoxicity.
32603032	1	12	theme	Long-term	99:107	arg1	use					109:111	Long-term use	99:111	Long-term use of nonsteroidal anti-inflammatory drugs (NSAIDs) for relieving inflammatory reactions	99:197	Long-term use of nonsteroidal anti-inflammatory drugs (NSAIDs) for relieving inflammatory reactions can lead to severe side effects.
32603032	1	13	theme	severe	211:216	arg1	effects					223:229	severe side effects	211:229	severe side effects	211:229	Long-term use of nonsteroidal anti-inflammatory drugs (NSAIDs) for relieving inflammatory reactions can lead to severe side effects.
32603032	3	14	theme	enzyme-responsive	354:370	arg1	prodrug					390:396	an enzyme-responsive anti-inflammatory prodrug	351:396	an enzyme-responsive anti-inflammatory prodrug capable of generating indomethacin upon the trigger of inflammation	351:464	In this work, an enzyme-responsive anti-inflammatory prodrug capable of generating indomethacin upon the trigger of inflammation is developed.
32603032	9	15	from	lipopolysaccharide	1298:1315	arg1	cells					1340:1344	RAW264.7 macrophage cells	1320:1344	RAW264.7 macrophage cells	1320:1344	Furthermore, the in vitro studies at the cellular level indicate that the enzyme-responsive prodrug can efficiently relieve inflammatory responses induced by lipopolysaccharide in RAW264.7 macrophage cells while indicating no cytotoxicity.
32603032	9	16	theme	cellular	1181:1188	arg1	level					1190:1194	the cellular level	1177:1194	the cellular level	1177:1194	Furthermore, the in vitro studies at the cellular level indicate that the enzyme-responsive prodrug can efficiently relieve inflammatory responses induced by lipopolysaccharide in RAW264.7 macrophage cells while indicating no cytotoxicity.
32603032	0	17	theme	Enzyme-Responsive	3:19	arg1	Prodrug					21:27	An Enzyme-Responsive Prodrug	0:27	An Enzyme-Responsive Prodrug with Inflammation-Triggered Therapeutic Drug Release Characteristics	0:96	An Enzyme-Responsive Prodrug with Inflammation-Triggered Therapeutic Drug Release Characteristics.
32603032	3	18	theme	inflammation	453:464	arg1	trigger					442:448	the trigger	438:448	the trigger of inflammation	438:464	In this work, an enzyme-responsive anti-inflammatory prodrug capable of generating indomethacin upon the trigger of inflammation is developed.
32603032	4	19	theme	N-	590:591	arg1	acrylamide					609:618	N-(2-hydroxyethyl) acrylamide	590:618	N-(2-hydroxyethyl) acrylamide	590:618	A monomer is first prepared after the esterification of carboxyl groups of indomethacin by hydroxyl groups of N-(2-hydroxyethyl) acrylamide.
32603032	1	20	theme	nonsteroidal	116:127	arg1	NSAIDs					154:159	NSAIDs	154:159	NSAIDs	154:159	Long-term use of nonsteroidal anti-inflammatory drugs (NSAIDs) for relieving inflammatory reactions can lead to severe side effects.
32603032	1	20	theme	nonsteroidal	116:127	arg1	drugs					147:151	nonsteroidal anti-inflammatory drugs	116:151	nonsteroidal anti-inflammatory drugs (NSAIDs)	116:160	Long-term use of nonsteroidal anti-inflammatory drugs (NSAIDs) for relieving inflammatory reactions can lead to severe side effects.
32603032	5	21	theme	radical	733:739	arg1	polymerization					741:754	free radical polymerization	728:754	free radical polymerization of the monomer	728:769	Then, a polymer prodrug, with indomethacin linked through ester bonds on the side chain, is synthesized by free radical polymerization of the monomer.
32603032	1	22	theme	side	218:221	arg1	effects					223:229	severe side effects	211:229	severe side effects	211:229	Long-term use of nonsteroidal anti-inflammatory drugs (NSAIDs) for relieving inflammatory reactions can lead to severe side effects.
32603032	3	23	theme	capable	398:404	arg1	prodrug					390:396	an enzyme-responsive anti-inflammatory prodrug	351:396	an enzyme-responsive anti-inflammatory prodrug capable of generating indomethacin upon the trigger of inflammation	351:464	In this work, an enzyme-responsive anti-inflammatory prodrug capable of generating indomethacin upon the trigger of inflammation is developed.
32603032	6	24	theme	drug	788:791	arg1	component					793:801	The therapeutic drug component	772:801	The therapeutic drug component	772:801	The therapeutic drug component can be triggered to release from the prodrug under the stimulation of cholesterol esterase, mimicking the inflammation environment.
32603032	1	25	theme	anti-inflammatory	129:145	arg1	NSAIDs					154:159	NSAIDs	154:159	NSAIDs	154:159	Long-term use of nonsteroidal anti-inflammatory drugs (NSAIDs) for relieving inflammatory reactions can lead to severe side effects.
32603032	1	25	theme	anti-inflammatory	129:145	arg1	drugs					147:151	nonsteroidal anti-inflammatory drugs	116:151	nonsteroidal anti-inflammatory drugs (NSAIDs)	116:160	Long-term use of nonsteroidal anti-inflammatory drugs (NSAIDs) for relieving inflammatory reactions can lead to severe side effects.
32603032	9	26	dep	in	1157:1158	arg1	vitro					1160:1164	vitro	1160:1164	vitro	1160:1164	Furthermore, the in vitro studies at the cellular level indicate that the enzyme-responsive prodrug can efficiently relieve inflammatory responses induced by lipopolysaccharide in RAW264.7 macrophage cells while indicating no cytotoxicity.
32603032	9	27	theme	enzyme-responsive	1214:1230	arg1	prodrug					1232:1238	the enzyme-responsive prodrug	1210:1238	the enzyme-responsive prodrug	1210:1238	Furthermore, the in vitro studies at the cellular level indicate that the enzyme-responsive prodrug can efficiently relieve inflammatory responses induced by lipopolysaccharide in RAW264.7 macrophage cells while indicating no cytotoxicity.
32603032	2	28	theme	NSAIDs	329:334	arg1	effects					318:324	the side effects	309:324	the side effects of NSAIDs	309:334	It is of great importance to configure new dosing strategies for alleviating the side effects of NSAIDs.
32603032	6	29	theme	esterase	885:892	arg1	stimulation					858:868	the stimulation	854:868	the stimulation of cholesterol esterase	854:892	The therapeutic drug component can be triggered to release from the prodrug under the stimulation of cholesterol esterase, mimicking the inflammation environment.
32603032	1	30	theme	drugs	147:151	arg1	use					109:111	Long-term use	99:111	Long-term use of nonsteroidal anti-inflammatory drugs (NSAIDs) for relieving inflammatory reactions	99:197	Long-term use of nonsteroidal anti-inflammatory drugs (NSAIDs) for relieving inflammatory reactions can lead to severe side effects.
32603032	0	31	theme	Therapeutic	57:67	arg1	Characteristics					82:96	Inflammation-Triggered Therapeutic Drug Release Characteristics	34:96	Inflammation-Triggered Therapeutic Drug Release Characteristics	34:96	An Enzyme-Responsive Prodrug with Inflammation-Triggered Therapeutic Drug Release Characteristics.
32603032	6	32	theme	cholesterol	873:883	arg1	esterase					885:892	cholesterol esterase	873:892	cholesterol esterase	873:892	The therapeutic drug component can be triggered to release from the prodrug under the stimulation of cholesterol esterase, mimicking the inflammation environment.
32603032	0	33	theme	Inflammation-Triggered	34:55	arg1	Characteristics					82:96	Inflammation-Triggered Therapeutic Drug Release Characteristics	34:96	Inflammation-Triggered Therapeutic Drug Release Characteristics	34:96	An Enzyme-Responsive Prodrug with Inflammation-Triggered Therapeutic Drug Release Characteristics.
32603032	5	34	from	bonds	685:689	arg1	chain					703:707	the side chain	694:707	the side chain	694:707	Then, a polymer prodrug, with indomethacin linked through ester bonds on the side chain, is synthesized by free radical polymerization of the monomer.
32603032	5	35	theme	free	728:731	arg1	polymerization					741:754	free radical polymerization	728:754	free radical polymerization of the monomer	728:769	Then, a polymer prodrug, with indomethacin linked through ester bonds on the side chain, is synthesized by free radical polymerization of the monomer.
32603032	6	36	theme	therapeutic	776:786	arg1	component					793:801	The therapeutic drug component	772:801	The therapeutic drug component	772:801	The therapeutic drug component can be triggered to release from the prodrug under the stimulation of cholesterol esterase, mimicking the inflammation environment.
32603032	0	37	theme	Release	74:80	arg1	Characteristics					82:96	Inflammation-Triggered Therapeutic Drug Release Characteristics	34:96	Inflammation-Triggered Therapeutic Drug Release Characteristics	34:96	An Enzyme-Responsive Prodrug with Inflammation-Triggered Therapeutic Drug Release Characteristics.
32603032	4	38	theme	indomethacin	555:566	arg1	indomethacin					555:566	indomethacin	555:566	indomethacin	555:566	A monomer is first prepared after the esterification of carboxyl groups of indomethacin by hydroxyl groups of N-(2-hydroxyethyl) acrylamide.
32603032	4	38	theme	indomethacin	555:566	arg1	groups					545:550	carboxyl groups	536:550	carboxyl groups of indomethacin	536:566	A monomer is first prepared after the esterification of carboxyl groups of indomethacin by hydroxyl groups of N-(2-hydroxyethyl) acrylamide.
32603032	2	39	theme	great	241:245	arg1	importance					247:256	great importance	241:256	great importance	241:256	It is of great importance to configure new dosing strategies for alleviating the side effects of NSAIDs.
32603032	0	40	with	Prodrug	21:27	arg1	Characteristics					82:96	Inflammation-Triggered Therapeutic Drug Release Characteristics	34:96	Inflammation-Triggered Therapeutic Drug Release Characteristics	34:96	An Enzyme-Responsive Prodrug with Inflammation-Triggered Therapeutic Drug Release Characteristics.
32603032	0	41	theme	Drug	69:72	arg1	Characteristics					82:96	Inflammation-Triggered Therapeutic Drug Release Characteristics	34:96	Inflammation-Triggered Therapeutic Drug Release Characteristics	34:96	An Enzyme-Responsive Prodrug with Inflammation-Triggered Therapeutic Drug Release Characteristics.
32603032	5	42	theme	side	698:701	arg1	chain					703:707	the side chain	694:707	the side chain	694:707	Then, a polymer prodrug, with indomethacin linked through ester bonds on the side chain, is synthesized by free radical polymerization of the monomer.
32603032	4	43	theme	hydroxyl	571:578	arg1	groups					580:585	hydroxyl groups	571:585	hydroxyl groups of N-(2-hydroxyethyl) acrylamide	571:618	A monomer is first prepared after the esterification of carboxyl groups of indomethacin by hydroxyl groups of N-(2-hydroxyethyl) acrylamide.
32603032	4	43	theme	hydroxyl	571:578	arg1	acrylamide					609:618	N-(2-hydroxyethyl) acrylamide	590:618	N-(2-hydroxyethyl) acrylamide	590:618	A monomer is first prepared after the esterification of carboxyl groups of indomethacin by hydroxyl groups of N-(2-hydroxyethyl) acrylamide.
32603032	7	44	theme	enzyme	1020:1025	arg1	absence					1005:1011	the absence	1001:1011	the absence of the enzyme	1001:1025	On the contrary, there is only a small amount of drug released in the absence of the enzyme.
32603032	9	45	theme	inflammatory	1264:1275	arg1	responses					1277:1285	inflammatory responses	1264:1285	inflammatory responses induced by lipopolysaccharide in RAW264.7 macrophage cells while indicating no cytotoxicity	1264:1377	Furthermore, the in vitro studies at the cellular level indicate that the enzyme-responsive prodrug can efficiently relieve inflammatory responses induced by lipopolysaccharide in RAW264.7 macrophage cells while indicating no cytotoxicity.
32603032	2	46	theme	dosing	275:280	arg1	strategies					282:291	new dosing strategies	271:291	new dosing strategies for alleviating the side effects of NSAIDs	271:334	It is of great importance to configure new dosing strategies for alleviating the side effects of NSAIDs.
32603032	9	47	from	level	1190:1194	arg1	studies					1166:1172	the in vitro studies	1153:1172	the in vitro studies at the cellular level	1153:1194	Furthermore, the in vitro studies at the cellular level indicate that the enzyme-responsive prodrug can efficiently relieve inflammatory responses induced by lipopolysaccharide in RAW264.7 macrophage cells while indicating no cytotoxicity.
32603032	5	48	theme	monomer	763:769	arg1	polymerization					741:754	free radical polymerization	728:754	free radical polymerization of the monomer	728:769	Then, a polymer prodrug, with indomethacin linked through ester bonds on the side chain, is synthesized by free radical polymerization of the monomer.
32603032	2	49	theme	new	271:273	arg1	strategies					282:291	new dosing strategies	271:291	new dosing strategies for alleviating the side effects of NSAIDs	271:334	It is of great importance to configure new dosing strategies for alleviating the side effects of NSAIDs.
32603032	8	50	theme	environment	1104:1114	arg1	stimulation					1086:1096	the stimulation	1082:1096	the stimulation of an environment mimicking inflammation	1082:1137	Therefore, the drug can be triggered to release under the stimulation of an environment mimicking inflammation.
32603032	4	51	theme	carboxyl	536:543	arg1	indomethacin					555:566	indomethacin	555:566	indomethacin	555:566	A monomer is first prepared after the esterification of carboxyl groups of indomethacin by hydroxyl groups of N-(2-hydroxyethyl) acrylamide.
32603032	4	51	theme	carboxyl	536:543	arg1	groups					545:550	carboxyl groups	536:550	carboxyl groups of indomethacin	536:566	A monomer is first prepared after the esterification of carboxyl groups of indomethacin by hydroxyl groups of N-(2-hydroxyethyl) acrylamide.
32603032	1	52	theme	inflammatory	176:187	arg1	reactions					189:197	inflammatory reactions	176:197	inflammatory reactions	176:197	Long-term use of nonsteroidal anti-inflammatory drugs (NSAIDs) for relieving inflammatory reactions can lead to severe side effects.
32603032	5	53	with	prodrug	637:643	arg1	indomethacin					651:662	indomethacin	651:662	indomethacin linked through ester bonds on the side chain	651:707	Then, a polymer prodrug, with indomethacin linked through ester bonds on the side chain, is synthesized by free radical polymerization of the monomer.
32092713	6	0	theme	scaffold	821:828	arg1	layer					804:808	the outer layer	794:808	the outer layer of the PCL scaffold	794:828	Applying a layer of gelatin methacryloyl (GelMA) hydrogel with an appropriate thickness on the outer layer of the PCL scaffold not only protects and supports the scaffold, but also improves the biocompatibility of the printed bile duct.
32092713	4	1	theme	bionics	529:535	arg1	effect					519:524	the effect	515:524	the effect of bionics	515:535	The PCL scaffold full of bile duct-like organ cells can achieve the effect of bionics, replacing the original bile duct to perform its proper function.
32092713	13	2	theme	PCL/GelMA/USPIO	1856:1870	arg1	scaffolds					1882:1890	PCL/GelMA/USPIO bile duct scaffolds	1856:1890	PCL/GelMA/USPIO bile duct scaffolds	1856:1890	In summary, PCL/GelMA/USPIO bile duct scaffolds are beneficial to the proliferation of cells on the scaffolds and can be used to construct biologically active artificial bile ducts.
32092713	13	3	used	used	1965:1968	arg2	scaffolds					1882:1890	PCL/GelMA/USPIO bile duct scaffolds	1856:1890	PCL/GelMA/USPIO bile duct scaffolds	1856:1890	In summary, PCL/GelMA/USPIO bile duct scaffolds are beneficial to the proliferation of cells on the scaffolds and can be used to construct biologically active artificial bile ducts.
32092713	13	4	from	summary	1847:1853	arg1	beneficial					1896:1905	beneficial	1896:1905	beneficial	1896:1905	In summary, PCL/GelMA/USPIO bile duct scaffolds are beneficial to the proliferation of cells on the scaffolds and can be used to construct biologically active artificial bile ducts.
32092713	6	5	theme	gelatin	723:729	arg1	hydrogel					752:759	gelatin methacryloyl (GelMA) hydrogel	723:759	gelatin methacryloyl (GelMA) hydrogel with an appropriate thickness on the outer layer of the PCL scaffold	723:828	Applying a layer of gelatin methacryloyl (GelMA) hydrogel with an appropriate thickness on the outer layer of the PCL scaffold not only protects and supports the scaffold, but also improves the biocompatibility of the printed bile duct.
32092713	13	6	theme	duct	1877:1880	arg1	scaffolds					1882:1890	PCL/GelMA/USPIO bile duct scaffolds	1856:1890	PCL/GelMA/USPIO bile duct scaffolds	1856:1890	In summary, PCL/GelMA/USPIO bile duct scaffolds are beneficial to the proliferation of cells on the scaffolds and can be used to construct biologically active artificial bile ducts.
32092713	2	7	theme	composite	251:259	arg1	scaffold					261:268	a tubular composite scaffold	241:268	a tubular composite scaffold using polycaprolactone (PCL) through the current 3D printing method	241:336	This study printed a tubular composite scaffold using polycaprolactone (PCL) through the current 3D printing method.
32092713	1	8	theme	bile	164:167	arg1	ducts					169:173	diseased bile ducts	155:173	diseased bile ducts	155:173	Making artificial bile ducts in vitro for repairing and replacing diseased bile ducts is an important concept in tissue engineering.
32092713	12	9	theme	stent	1825:1829	arg1	degradation					1806:1816	the degradation	1802:1816	the degradation of the stent in the body	1802:1841	USPIO can achieve MRI imaging in situ and nondestructively monitor the degradation of the stent in the body.
32092713	6	10	from	thickness	781:789	arg1	layer					804:808	the outer layer	794:808	the outer layer of the PCL scaffold	794:828	Applying a layer of gelatin methacryloyl (GelMA) hydrogel with an appropriate thickness on the outer layer of the PCL scaffold not only protects and supports the scaffold, but also improves the biocompatibility of the printed bile duct.
32092713	7	11	theme	iron	982:985	arg1	USPIO					994:998	USPIO	994:998	USPIO	994:998	In addition, ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles dispersed in GelMA served as the contrast agent to monitor the repair of the lesion site and the degradation of the bile duct in real time by magnetic resonance imaging (MRI).
32092713	7	11	theme	iron	982:985	arg1	oxide					987:991	ultrasmall superparamagnetic iron oxide	953:991	ultrasmall superparamagnetic iron oxide (USPIO)	953:999	In addition, ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles dispersed in GelMA served as the contrast agent to monitor the repair of the lesion site and the degradation of the bile duct in real time by magnetic resonance imaging (MRI).
32092713	11	12	dep	the	1702:1704	arg1	needs					1706:1710	needs	1706:1710	needs	1706:1710	Thus, the mechanical properties of it can meet the needs of human implantation.
32092713	8	13	theme	real-time	1291:1299	arg1	property					1313:1320	a real-time MRI imaging property	1289:1320	a real-time MRI imaging property	1289:1320	In this study, a tubular composite scaffold that could reconstruct bile duct function and possess a real-time MRI imaging property was constructed by 3D printing.
32092713	4	14	theme	proper	586:591	arg1	function					593:600	its proper function	582:600	its proper function	582:600	The PCL scaffold full of bile duct-like organ cells can achieve the effect of bionics, replacing the original bile duct to perform its proper function.
32092713	7	15	theme	ultrasmall	953:962	arg1	USPIO					994:998	USPIO	994:998	USPIO	994:998	In addition, ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles dispersed in GelMA served as the contrast agent to monitor the repair of the lesion site and the degradation of the bile duct in real time by magnetic resonance imaging (MRI).
32092713	7	15	theme	ultrasmall	953:962	arg1	oxide					987:991	ultrasmall superparamagnetic iron oxide	953:991	ultrasmall superparamagnetic iron oxide (USPIO)	953:999	In addition, ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles dispersed in GelMA served as the contrast agent to monitor the repair of the lesion site and the degradation of the bile duct in real time by magnetic resonance imaging (MRI).
32092713	1	16	from	concept	191:197	arg1	engineering					209:219	tissue engineering	202:219	tissue engineering	202:219	Making artificial bile ducts in vitro for repairing and replacing diseased bile ducts is an important concept in tissue engineering.
32092713	8	17	theme	imaging	1305:1311	arg1	property					1313:1320	a real-time MRI imaging property	1289:1320	a real-time MRI imaging property	1289:1320	In this study, a tubular composite scaffold that could reconstruct bile duct function and possess a real-time MRI imaging property was constructed by 3D printing.
32092713	13	18	theme	artificial	2003:2012	arg1	ducts					2019:2023	biologically active artificial bile ducts	1983:2023	biologically active artificial bile ducts	1983:2023	In summary, PCL/GelMA/USPIO bile duct scaffolds are beneficial to the proliferation of cells on the scaffolds and can be used to construct biologically active artificial bile ducts.
32092713	6	19	theme	printed	921:927	arg1	duct					934:937	the printed bile duct	917:937	the printed bile duct	917:937	Applying a layer of gelatin methacryloyl (GelMA) hydrogel with an appropriate thickness on the outer layer of the PCL scaffold not only protects and supports the scaffold, but also improves the biocompatibility of the printed bile duct.
32092713	4	20	theme	PCL	455:457	arg1	scaffold					459:466	The PCL scaffold full	451:471	The PCL scaffold full of bile duct-like organ cells	451:501	The PCL scaffold full of bile duct-like organ cells can achieve the effect of bionics, replacing the original bile duct to perform its proper function.
32092713	0	21	theme	bile	71:74	arg1	repair					81:86	MRI-guided bile duct repair	60:86	MRI-guided bile duct repair	60:86	A novel 3D printing PCL/GelMA scaffold containing USPIO for MRI-guided bile duct repair.
32092713	9	22	theme	co-culture	1375:1384	arg1	days					1363:1366	13 days	1360:1366	13 days of the co-culture of bone marrow derived stem cells (BMSCs)	1360:1426	After 13 days of the co-culture of bone marrow derived stem cells (BMSCs), the survival rate of the BMSCs was greater than 95%, and the coverage of the BMSCs was as high as 90%.
32092713	4	23	theme	full	468:471	arg1	scaffold					459:466	The PCL scaffold full	451:471	The PCL scaffold full of bile duct-like organ cells	451:501	The PCL scaffold full of bile duct-like organ cells can achieve the effect of bionics, replacing the original bile duct to perform its proper function.
32092713	7	24	theme	lesion	1092:1097	arg1	site					1099:1102	the lesion site	1088:1102	the lesion site	1088:1102	In addition, ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles dispersed in GelMA served as the contrast agent to monitor the repair of the lesion site and the degradation of the bile duct in real time by magnetic resonance imaging (MRI).
32092713	9	25	theme	bone	1389:1392	arg1	marrow					1394:1399	bone marrow	1389:1399	bone marrow	1389:1399	After 13 days of the co-culture of bone marrow derived stem cells (BMSCs), the survival rate of the BMSCs was greater than 95%, and the coverage of the BMSCs was as high as 90%.
32092713	3	26	theme	duct	396:399	arg1	cells					378:382	the organoid cells	365:382	the organoid cells of the bile duct	365:399	It served as a matrix for the organoid cells of the bile duct to proliferation, migration, and differentiation.
32092713	9	27	theme	derived	1401:1407	arg1	cells					1414:1418	bone marrow derived stem cells	1389:1418	bone marrow derived stem cells (BMSCs)	1389:1426	After 13 days of the co-culture of bone marrow derived stem cells (BMSCs), the survival rate of the BMSCs was greater than 95%, and the coverage of the BMSCs was as high as 90%.
32092713	9	27	theme	derived	1401:1407	arg1	BMSCs					1421:1425	BMSCs	1421:1425	BMSCs	1421:1425	After 13 days of the co-culture of bone marrow derived stem cells (BMSCs), the survival rate of the BMSCs was greater than 95%, and the coverage of the BMSCs was as high as 90%.
32092713	8	28	contain	possess	1281:1287	arg2	property					1313:1320	a real-time MRI imaging property	1289:1320	a real-time MRI imaging property	1289:1320	In this study, a tubular composite scaffold that could reconstruct bile duct function and possess a real-time MRI imaging property was constructed by 3D printing.
32092713	8	28	contain	possess	1281:1287	arg1	scaffold					1226:1233	a tubular composite scaffold	1206:1233	a tubular composite scaffold that could reconstruct bile duct function and possess a real-time MRI imaging property	1206:1320	In this study, a tubular composite scaffold that could reconstruct bile duct function and possess a real-time MRI imaging property was constructed by 3D printing.
32092713	8	29	theme	composite	1216:1224	arg1	scaffold					1226:1233	a tubular composite scaffold	1206:1233	a tubular composite scaffold that could reconstruct bile duct function and possess a real-time MRI imaging property	1206:1320	In this study, a tubular composite scaffold that could reconstruct bile duct function and possess a real-time MRI imaging property was constructed by 3D printing.
32092713	5	30	used	used	683:686	arg2	hydrogels					663:671	hydrogels	663:671	hydrogels	663:671	In order to enrich the performance of the tubular scaffold, hydrogels were also used in this study.
32092713	12	31	from	stent	1825:1829	arg1	body					1838:1841	the body	1834:1841	the body	1834:1841	USPIO can achieve MRI imaging in situ and nondestructively monitor the degradation of the stent in the body.
32092713	9	32	theme	cells	1414:1418	arg1	co-culture					1375:1384	the co-culture	1371:1384	the co-culture of bone marrow derived stem cells (BMSCs)	1371:1426	After 13 days of the co-culture of bone marrow derived stem cells (BMSCs), the survival rate of the BMSCs was greater than 95%, and the coverage of the BMSCs was as high as 90%.
32092713	0	33	theme	3D	8:9	arg1	scaffold					30:37	A novel 3D printing PCL/GelMA scaffold	0:37	A novel 3D printing PCL/GelMA scaffold	0:37	A novel 3D printing PCL/GelMA scaffold containing USPIO for MRI-guided bile duct repair.
32092713	1	34	theme	artificial	96:105	arg1	ducts					112:116	artificial bile ducts	96:116	artificial bile ducts	96:116	Making artificial bile ducts in vitro for repairing and replacing diseased bile ducts is an important concept in tissue engineering.
32092713	7	35	theme	contrast	1048:1055	arg1	agent					1057:1061	the contrast agent	1044:1061	the contrast agent	1044:1061	In addition, ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles dispersed in GelMA served as the contrast agent to monitor the repair of the lesion site and the degradation of the bile duct in real time by magnetic resonance imaging (MRI).
32092713	0	36	theme	PCL/GelMA	20:28	arg1	scaffold					30:37	A novel 3D printing PCL/GelMA scaffold	0:37	A novel 3D printing PCL/GelMA scaffold	0:37	A novel 3D printing PCL/GelMA scaffold containing USPIO for MRI-guided bile duct repair.
32092713	6	37	theme	appropriate	769:779	arg1	thickness					781:789	an appropriate thickness	766:789	an appropriate thickness on the outer layer of the PCL scaffold	766:828	Applying a layer of gelatin methacryloyl (GelMA) hydrogel with an appropriate thickness on the outer layer of the PCL scaffold not only protects and supports the scaffold, but also improves the biocompatibility of the printed bile duct.
32092713	6	38	with	hydrogel	752:759	arg1	thickness					781:789	an appropriate thickness	766:789	an appropriate thickness on the outer layer of the PCL scaffold	766:828	Applying a layer of gelatin methacryloyl (GelMA) hydrogel with an appropriate thickness on the outer layer of the PCL scaffold not only protects and supports the scaffold, but also improves the biocompatibility of the printed bile duct.
32092713	4	39	theme	bile	476:479	arg1	cells					497:501	bile duct-like organ cells	476:501	bile duct-like organ cells	476:501	The PCL scaffold full of bile duct-like organ cells can achieve the effect of bionics, replacing the original bile duct to perform its proper function.
32092713	13	40	from	beneficial	1896:1905	arg1	summary					1847:1853	summary	1847:1853	summary	1847:1853	In summary, PCL/GelMA/USPIO bile duct scaffolds are beneficial to the proliferation of cells on the scaffolds and can be used to construct biologically active artificial bile ducts.
32092713	8	41	theme	duct	1263:1266	arg1	function					1268:1275	bile duct function	1258:1275	bile duct function	1258:1275	In this study, a tubular composite scaffold that could reconstruct bile duct function and possess a real-time MRI imaging property was constructed by 3D printing.
32092713	2	42	theme	printing	322:329	arg1	method					331:336	the current 3D printing method	307:336	the current 3D printing method	307:336	This study printed a tubular composite scaffold using polycaprolactone (PCL) through the current 3D printing method.
32092713	9	43	theme	survival	1433:1440	arg1	%					1479:1479	greater than 95%	1464:1479	greater than 95%	1464:1479	After 13 days of the co-culture of bone marrow derived stem cells (BMSCs), the survival rate of the BMSCs was greater than 95%, and the coverage of the BMSCs was as high as 90%.
32092713	9	43	theme	survival	1433:1440	arg1	rate					1442:1445	the survival rate	1429:1445	the survival rate of the BMSCs	1429:1458	After 13 days of the co-culture of bone marrow derived stem cells (BMSCs), the survival rate of the BMSCs was greater than 95%, and the coverage of the BMSCs was as high as 90%.
32092713	4	44	theme	organ	491:495	arg1	cells					497:501	bile duct-like organ cells	476:501	bile duct-like organ cells	476:501	The PCL scaffold full of bile duct-like organ cells can achieve the effect of bionics, replacing the original bile duct to perform its proper function.
32092713	2	45	theme	current	311:317	arg1	method					331:336	the current 3D printing method	307:336	the current 3D printing method	307:336	This study printed a tubular composite scaffold using polycaprolactone (PCL) through the current 3D printing method.
32092713	1	46	theme	tissue	202:207	arg1	engineering					209:219	tissue engineering	202:219	tissue engineering	202:219	Making artificial bile ducts in vitro for repairing and replacing diseased bile ducts is an important concept in tissue engineering.
32092713	7	47	theme	real	1144:1147	arg1	time					1149:1152	real time	1144:1152	real time	1144:1152	In addition, ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles dispersed in GelMA served as the contrast agent to monitor the repair of the lesion site and the degradation of the bile duct in real time by magnetic resonance imaging (MRI).
32092713	7	48	theme	resonance	1166:1174	arg1	imaging					1176:1182	magnetic resonance imaging	1157:1182	magnetic resonance imaging (MRI)	1157:1188	In addition, ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles dispersed in GelMA served as the contrast agent to monitor the repair of the lesion site and the degradation of the bile duct in real time by magnetic resonance imaging (MRI).
32092713	7	48	theme	resonance	1166:1174	arg1	MRI					1185:1187	MRI	1185:1187	MRI	1185:1187	In addition, ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles dispersed in GelMA served as the contrast agent to monitor the repair of the lesion site and the degradation of the bile duct in real time by magnetic resonance imaging (MRI).
32092713	9	49	theme	BMSCs	1454:1458	arg1	%					1479:1479	greater than 95%	1464:1479	greater than 95%	1464:1479	After 13 days of the co-culture of bone marrow derived stem cells (BMSCs), the survival rate of the BMSCs was greater than 95%, and the coverage of the BMSCs was as high as 90%.
32092713	9	49	theme	BMSCs	1454:1458	arg1	rate					1442:1445	the survival rate	1429:1445	the survival rate of the BMSCs	1429:1458	After 13 days of the co-culture of bone marrow derived stem cells (BMSCs), the survival rate of the BMSCs was greater than 95%, and the coverage of the BMSCs was as high as 90%.
32092713	7	50	theme	duct	1136:1139	arg1	repair					1078:1083	the repair	1074:1083	the repair of the lesion site	1074:1102	In addition, ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles dispersed in GelMA served as the contrast agent to monitor the repair of the lesion site and the degradation of the bile duct in real time by magnetic resonance imaging (MRI).
32092713	7	50	theme	duct	1136:1139	arg1	degradation					1112:1122	the degradation	1108:1122	the degradation of the bile duct	1108:1139	In addition, ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles dispersed in GelMA served as the contrast agent to monitor the repair of the lesion site and the degradation of the bile duct in real time by magnetic resonance imaging (MRI).
32092713	4	51	theme	bile	561:564	arg1	duct					566:569	the original bile duct	548:569	the original bile duct to perform its proper function	548:600	The PCL scaffold full of bile duct-like organ cells can achieve the effect of bionics, replacing the original bile duct to perform its proper function.
32092713	7	52	theme	site	1099:1102	arg1	repair					1078:1083	the repair	1074:1083	the repair of the lesion site	1074:1102	In addition, ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles dispersed in GelMA served as the contrast agent to monitor the repair of the lesion site and the degradation of the bile duct in real time by magnetic resonance imaging (MRI).
32092713	7	52	theme	site	1099:1102	arg1	degradation					1112:1122	the degradation	1108:1122	the degradation of the bile duct	1108:1139	In addition, ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles dispersed in GelMA served as the contrast agent to monitor the repair of the lesion site and the degradation of the bile duct in real time by magnetic resonance imaging (MRI).
32092713	12	53	theme	MRI	1753:1755	arg1	imaging					1757:1763	MRI imaging	1753:1763	MRI imaging	1753:1763	USPIO can achieve MRI imaging in situ and nondestructively monitor the degradation of the stent in the body.
32092713	6	54	theme	PCL	817:819	arg1	scaffold					821:828	the PCL scaffold	813:828	the PCL scaffold	813:828	Applying a layer of gelatin methacryloyl (GelMA) hydrogel with an appropriate thickness on the outer layer of the PCL scaffold not only protects and supports the scaffold, but also improves the biocompatibility of the printed bile duct.
32092713	6	55	theme	methacryloyl	731:742	arg1	hydrogel					752:759	gelatin methacryloyl (GelMA) hydrogel	723:759	gelatin methacryloyl (GelMA) hydrogel with an appropriate thickness on the outer layer of the PCL scaffold	723:828	Applying a layer of gelatin methacryloyl (GelMA) hydrogel with an appropriate thickness on the outer layer of the PCL scaffold not only protects and supports the scaffold, but also improves the biocompatibility of the printed bile duct.
32092713	13	56	theme	bile	1872:1875	arg1	scaffolds					1882:1890	PCL/GelMA/USPIO bile duct scaffolds	1856:1890	PCL/GelMA/USPIO bile duct scaffolds	1856:1890	In summary, PCL/GelMA/USPIO bile duct scaffolds are beneficial to the proliferation of cells on the scaffolds and can be used to construct biologically active artificial bile ducts.
32092713	9	57	link	derived	1401:1407	arg1	cells					1414:1418	bone marrow derived stem cells	1389:1418	bone marrow derived stem cells (BMSCs)	1389:1426	After 13 days of the co-culture of bone marrow derived stem cells (BMSCs), the survival rate of the BMSCs was greater than 95%, and the coverage of the BMSCs was as high as 90%.
32092713	9	57	link	derived	1401:1407	arg1	BMSCs					1421:1425	BMSCs	1421:1425	BMSCs	1421:1425	After 13 days of the co-culture of bone marrow derived stem cells (BMSCs), the survival rate of the BMSCs was greater than 95%, and the coverage of the BMSCs was as high as 90%.
32092713	11	58	theme	human	1715:1719	arg1	implantation					1721:1732	human implantation	1715:1732	human implantation	1715:1732	Thus, the mechanical properties of it can meet the needs of human implantation.
32092713	1	59	theme	diseased	155:162	arg1	ducts					169:173	diseased bile ducts	155:173	diseased bile ducts	155:173	Making artificial bile ducts in vitro for repairing and replacing diseased bile ducts is an important concept in tissue engineering.
32092713	3	60	theme	organoid	369:376	arg1	cells					378:382	the organoid cells	365:382	the organoid cells of the bile duct	365:399	It served as a matrix for the organoid cells of the bile duct to proliferation, migration, and differentiation.
32092713	6	61	theme	outer	798:802	arg1	layer					804:808	the outer layer	794:808	the outer layer of the PCL scaffold	794:828	Applying a layer of gelatin methacryloyl (GelMA) hydrogel with an appropriate thickness on the outer layer of the PCL scaffold not only protects and supports the scaffold, but also improves the biocompatibility of the printed bile duct.
32092713	7	62	dep	nanoparticles	1001:1013	arg1	dispersed					1015:1023	dispersed	1015:1023	nanoparticles dispersed in GelMA served as the contrast agent to monitor the repair of the lesion site and the degradation of the bile duct in real time by magnetic resonance imaging (MRI)	1001:1188	In addition, ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles dispersed in GelMA served as the contrast agent to monitor the repair of the lesion site and the degradation of the bile duct in real time by magnetic resonance imaging (MRI).
32092713	7	63	theme	superparamagnetic	964:980	arg1	USPIO					994:998	USPIO	994:998	USPIO	994:998	In addition, ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles dispersed in GelMA served as the contrast agent to monitor the repair of the lesion site and the degradation of the bile duct in real time by magnetic resonance imaging (MRI).
32092713	7	63	theme	superparamagnetic	964:980	arg1	oxide					987:991	ultrasmall superparamagnetic iron oxide	953:991	ultrasmall superparamagnetic iron oxide (USPIO)	953:999	In addition, ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles dispersed in GelMA served as the contrast agent to monitor the repair of the lesion site and the degradation of the bile duct in real time by magnetic resonance imaging (MRI).
32092713	10	64	theme	same	1539:1542	arg1	time					1544:1547	the same time	1535:1547	the same time	1535:1547	At the same time, the compression modulus of the stent could reach 17.41 kPa and the Young's modulus could reach 5.03 kPa.
32092713	13	65	theme	bile	2014:2017	arg1	ducts					2019:2023	biologically active artificial bile ducts	1983:2023	biologically active artificial bile ducts	1983:2023	In summary, PCL/GelMA/USPIO bile duct scaffolds are beneficial to the proliferation of cells on the scaffolds and can be used to construct biologically active artificial bile ducts.
32092713	8	66	theme	MRI	1301:1303	arg1	property					1313:1320	a real-time MRI imaging property	1289:1320	a real-time MRI imaging property	1289:1320	In this study, a tubular composite scaffold that could reconstruct bile duct function and possess a real-time MRI imaging property was constructed by 3D printing.
32092713	5	67	theme	tubular	645:651	arg1	scaffold					653:660	the tubular scaffold	641:660	the tubular scaffold	641:660	In order to enrich the performance of the tubular scaffold, hydrogels were also used in this study.
32092713	11	68	theme	it	1690:1691	arg1	properties					1676:1685	the mechanical properties	1661:1685	the mechanical properties of it	1661:1691	Thus, the mechanical properties of it can meet the needs of human implantation.
32092713	9	69	theme	BMSCs	1506:1510	arg1	coverage					1490:1497	the coverage	1486:1497	the coverage of the BMSCs	1486:1510	After 13 days of the co-culture of bone marrow derived stem cells (BMSCs), the survival rate of the BMSCs was greater than 95%, and the coverage of the BMSCs was as high as 90%.
32092713	9	69	theme	BMSCs	1506:1510	arg1	high					1519:1522	high	1519:1522	high	1519:1522	After 13 days of the co-culture of bone marrow derived stem cells (BMSCs), the survival rate of the BMSCs was greater than 95%, and the coverage of the BMSCs was as high as 90%.
32092713	6	70	theme	duct	934:937	arg1	biocompatibility					897:912	the biocompatibility	893:912	the biocompatibility of the printed bile duct	893:937	Applying a layer of gelatin methacryloyl (GelMA) hydrogel with an appropriate thickness on the outer layer of the PCL scaffold not only protects and supports the scaffold, but also improves the biocompatibility of the printed bile duct.
32092713	10	71	theme	compression	1554:1564	arg1	modulus					1566:1572	the compression modulus	1550:1572	the compression modulus of the stent	1550:1585	At the same time, the compression modulus of the stent could reach 17.41 kPa and the Young's modulus could reach 5.03 kPa.
32092713	12	72	from	degradation	1806:1816	arg1	body					1838:1841	the body	1834:1841	the body	1834:1841	USPIO can achieve MRI imaging in situ and nondestructively monitor the degradation of the stent in the body.
32092713	1	73	theme	bile	107:110	arg1	ducts					112:116	artificial bile ducts	96:116	artificial bile ducts	96:116	Making artificial bile ducts in vitro for repairing and replacing diseased bile ducts is an important concept in tissue engineering.
32092713	9	74	dep	derived	1401:1407	arg1	marrow					1394:1399	bone marrow	1389:1399	bone marrow	1389:1399	After 13 days of the co-culture of bone marrow derived stem cells (BMSCs), the survival rate of the BMSCs was greater than 95%, and the coverage of the BMSCs was as high as 90%.
32092713	2	75	theme	tubular	243:249	arg1	scaffold					261:268	a tubular composite scaffold	241:268	a tubular composite scaffold using polycaprolactone (PCL) through the current 3D printing method	241:336	This study printed a tubular composite scaffold using polycaprolactone (PCL) through the current 3D printing method.
32092713	13	76	theme	active	1996:2001	arg1	ducts					2019:2023	biologically active artificial bile ducts	1983:2023	biologically active artificial bile ducts	1983:2023	In summary, PCL/GelMA/USPIO bile duct scaffolds are beneficial to the proliferation of cells on the scaffolds and can be used to construct biologically active artificial bile ducts.
32092713	6	77	theme	bile	929:932	arg1	duct					934:937	the printed bile duct	917:937	the printed bile duct	917:937	Applying a layer of gelatin methacryloyl (GelMA) hydrogel with an appropriate thickness on the outer layer of the PCL scaffold not only protects and supports the scaffold, but also improves the biocompatibility of the printed bile duct.
32092713	8	78	theme	3D	1341:1342	arg1	printing					1344:1351	3D printing	1341:1351	3D printing	1341:1351	In this study, a tubular composite scaffold that could reconstruct bile duct function and possess a real-time MRI imaging property was constructed by 3D printing.
32092713	0	79	theme	MRI-guided	60:69	arg1	repair					81:86	MRI-guided bile duct repair	60:86	MRI-guided bile duct repair	60:86	A novel 3D printing PCL/GelMA scaffold containing USPIO for MRI-guided bile duct repair.
32092713	10	80	theme	stent	1581:1585	arg1	modulus					1566:1572	the compression modulus	1550:1572	the compression modulus of the stent	1550:1585	At the same time, the compression modulus of the stent could reach 17.41 kPa and the Young's modulus could reach 5.03 kPa.
32092713	0	81	theme	duct	76:79	arg1	repair					81:86	MRI-guided bile duct repair	60:86	MRI-guided bile duct repair	60:86	A novel 3D printing PCL/GelMA scaffold containing USPIO for MRI-guided bile duct repair.
32092713	13	82	from	proliferation	1914:1926	arg1	scaffolds					1944:1952	the scaffolds	1940:1952	the scaffolds	1940:1952	In summary, PCL/GelMA/USPIO bile duct scaffolds are beneficial to the proliferation of cells on the scaffolds and can be used to construct biologically active artificial bile ducts.
32092713	1	83	theme	important	181:189	arg1	concept					191:197	an important concept	178:197	an important concept in tissue engineering	178:219	Making artificial bile ducts in vitro for repairing and replacing diseased bile ducts is an important concept in tissue engineering.
32092713	0	84	theme	novel	2:6	arg1	scaffold					30:37	A novel 3D printing PCL/GelMA scaffold	0:37	A novel 3D printing PCL/GelMA scaffold	0:37	A novel 3D printing PCL/GelMA scaffold containing USPIO for MRI-guided bile duct repair.
32092713	9	85	theme	stem	1409:1412	arg1	cells					1414:1418	bone marrow derived stem cells	1389:1418	bone marrow derived stem cells (BMSCs)	1389:1426	After 13 days of the co-culture of bone marrow derived stem cells (BMSCs), the survival rate of the BMSCs was greater than 95%, and the coverage of the BMSCs was as high as 90%.
32092713	9	85	theme	stem	1409:1412	arg1	BMSCs					1421:1425	BMSCs	1421:1425	BMSCs	1421:1425	After 13 days of the co-culture of bone marrow derived stem cells (BMSCs), the survival rate of the BMSCs was greater than 95%, and the coverage of the BMSCs was as high as 90%.
32092713	8	86	theme	tubular	1208:1214	arg1	scaffold					1226:1233	a tubular composite scaffold	1206:1233	a tubular composite scaffold that could reconstruct bile duct function and possess a real-time MRI imaging property	1206:1320	In this study, a tubular composite scaffold that could reconstruct bile duct function and possess a real-time MRI imaging property was constructed by 3D printing.
32092713	3	87	theme	bile	391:394	arg1	duct					396:399	the bile duct	387:399	the bile duct	387:399	It served as a matrix for the organoid cells of the bile duct to proliferation, migration, and differentiation.
32092713	0	88	theme	printing	11:18	arg1	scaffold					30:37	A novel 3D printing PCL/GelMA scaffold	0:37	A novel 3D printing PCL/GelMA scaffold	0:37	A novel 3D printing PCL/GelMA scaffold containing USPIO for MRI-guided bile duct repair.
32092713	12	89	from	body	1838:1841	arg1	degradation					1806:1816	the degradation	1802:1816	the degradation of the stent in the body	1802:1841	USPIO can achieve MRI imaging in situ and nondestructively monitor the degradation of the stent in the body.
32092713	11	90	theme	mechanical	1665:1674	arg1	properties					1676:1685	the mechanical properties	1661:1685	the mechanical properties of it	1661:1691	Thus, the mechanical properties of it can meet the needs of human implantation.
32092713	7	91	theme	magnetic	1157:1164	arg1	imaging					1176:1182	magnetic resonance imaging	1157:1182	magnetic resonance imaging (MRI)	1157:1188	In addition, ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles dispersed in GelMA served as the contrast agent to monitor the repair of the lesion site and the degradation of the bile duct in real time by magnetic resonance imaging (MRI).
32092713	7	91	theme	magnetic	1157:1164	arg1	MRI					1185:1187	MRI	1185:1187	MRI	1185:1187	In addition, ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles dispersed in GelMA served as the contrast agent to monitor the repair of the lesion site and the degradation of the bile duct in real time by magnetic resonance imaging (MRI).
32092713	4	92	theme	duct-like	481:489	arg1	cells					497:501	bile duct-like organ cells	476:501	bile duct-like organ cells	476:501	The PCL scaffold full of bile duct-like organ cells can achieve the effect of bionics, replacing the original bile duct to perform its proper function.
32092713	5	93	theme	scaffold	653:660	arg1	performance					626:636	the performance	622:636	the performance of the tubular scaffold	622:660	In order to enrich the performance of the tubular scaffold, hydrogels were also used in this study.
32092713	4	94	theme	cells	497:501	arg1	scaffold					459:466	The PCL scaffold full	451:471	The PCL scaffold full of bile duct-like organ cells	451:501	The PCL scaffold full of bile duct-like organ cells can achieve the effect of bionics, replacing the original bile duct to perform its proper function.
32092713	8	95	theme	bile	1258:1261	arg1	function					1268:1275	bile duct function	1258:1275	bile duct function	1258:1275	In this study, a tubular composite scaffold that could reconstruct bile duct function and possess a real-time MRI imaging property was constructed by 3D printing.
32092713	2	96	theme	3D	319:320	arg1	method					331:336	the current 3D printing method	307:336	the current 3D printing method	307:336	This study printed a tubular composite scaffold using polycaprolactone (PCL) through the current 3D printing method.
32092713	13	97	theme	cells	1931:1935	arg1	proliferation					1914:1926	the proliferation	1910:1926	the proliferation of cells on the scaffolds	1910:1952	In summary, PCL/GelMA/USPIO bile duct scaffolds are beneficial to the proliferation of cells on the scaffolds and can be used to construct biologically active artificial bile ducts.
32092713	6	98	theme	hydrogel	752:759	arg1	layer					714:718	a layer	712:718	a layer of gelatin methacryloyl (GelMA) hydrogel with an appropriate thickness on the outer layer of the PCL scaffold	712:828	Applying a layer of gelatin methacryloyl (GelMA) hydrogel with an appropriate thickness on the outer layer of the PCL scaffold not only protects and supports the scaffold, but also improves the biocompatibility of the printed bile duct.
32092713	4	99	theme	original	552:559	arg1	duct					566:569	the original bile duct	548:569	the original bile duct to perform its proper function	548:600	The PCL scaffold full of bile duct-like organ cells can achieve the effect of bionics, replacing the original bile duct to perform its proper function.
32092713	6	100	theme	GelMA	745:749	arg1	hydrogel					752:759	gelatin methacryloyl (GelMA) hydrogel	723:759	gelatin methacryloyl (GelMA) hydrogel with an appropriate thickness on the outer layer of the PCL scaffold	723:828	Applying a layer of gelatin methacryloyl (GelMA) hydrogel with an appropriate thickness on the outer layer of the PCL scaffold not only protects and supports the scaffold, but also improves the biocompatibility of the printed bile duct.
32092713	7	101	theme	bile	1131:1134	arg1	duct					1136:1139	the bile duct	1127:1139	the bile duct	1127:1139	In addition, ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles dispersed in GelMA served as the contrast agent to monitor the repair of the lesion site and the degradation of the bile duct in real time by magnetic resonance imaging (MRI).
33605846	0	0	theme	vaccines	82:89	arg1	impact					45:50	the comparable impact	30:50	the comparable impact of the pneumococcal conjugate vaccines PHiD-CV and PCV13 on overall pneumococcal disease	30:139	Exploring the evidence behind the comparable impact of the pneumococcal conjugate vaccines PHiD-CV and PCV13 on overall pneumococcal disease.
33605846	9	1	with	burden	1532:1537	arg1	vaccines					1549:1556	both vaccines	1544:1556	both vaccines	1544:1556	This results in a similar residual overall disease burden with both vaccines.
33605846	4	2	from	differences	764:774	arg1	composition					788:798	serotype composition	779:798	serotype composition	779:798	This commentary summarizes the evidence of comparability between PHiD-CV and PCV13 and explores why differences in serotype composition may not necessarily translate into a differential clinical impact.
33605846	2	3	theme	influenzae	374:383	arg1	PHiD-CV					414:420	PHiD-CV	414:420	PHiD-CV	414:420	Two PCVs are widely available for infant vaccination: the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) and the 13-valent PCV (PCV13).
33605846	2	3	theme	influenzae	374:383	arg1	vaccine					405:411	the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine	332:411	the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV)	332:421	Two PCVs are widely available for infant vaccination: the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) and the 13-valent PCV (PCV13).
33605846	1	4	theme	pneumococcal	249:260	arg1	burden					270:275	the overall pneumococcal disease burden	237:275	the overall pneumococcal disease burden	237:275	The worldwide implementation of pneumococcal conjugate vaccines (PCVs) in children has reduced the overall pneumococcal disease burden.
33605846	0	5	theme	conjugate	72:80	arg1	vaccines					82:89	the pneumococcal conjugate vaccines PHiD-CV and PCV13	55:107	the pneumococcal conjugate vaccines PHiD-CV and PCV13	55:107	Exploring the evidence behind the comparable impact of the pneumococcal conjugate vaccines PHiD-CV and PCV13 on overall pneumococcal disease.
33605846	0	5	theme	conjugate	72:80	arg1	PCV13					103:107	PCV13	103:107	PCV13	103:107	Exploring the evidence behind the comparable impact of the pneumococcal conjugate vaccines PHiD-CV and PCV13 on overall pneumococcal disease.
33605846	0	5	theme	conjugate	72:80	arg1	PHiD-CV					91:97	PHiD-CV	91:97	PHiD-CV	91:97	Exploring the evidence behind the comparable impact of the pneumococcal conjugate vaccines PHiD-CV and PCV13 on overall pneumococcal disease.
33605846	1	6	from	implementation	156:169	arg1	children					216:223	children	216:223	children	216:223	The worldwide implementation of pneumococcal conjugate vaccines (PCVs) in children has reduced the overall pneumococcal disease burden.
33605846	2	7	theme	Haemophilus	362:372	arg1	PHiD-CV					414:420	PHiD-CV	414:420	PHiD-CV	414:420	Two PCVs are widely available for infant vaccination: the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) and the 13-valent PCV (PCV13).
33605846	2	7	theme	Haemophilus	362:372	arg1	vaccine					405:411	the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine	332:411	the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV)	332:421	Two PCVs are widely available for infant vaccination: the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) and the 13-valent PCV (PCV13).
33605846	8	8	from	replacement	1370:1380	arg1	settings					1408:1415	settings	1408:1415	settings	1408:1415	While protection against 19A is higher for PCV13 than PHiD-CV, replacement by non-PCV13 serotypes in settings with a PCV13 program appears to compensate for this difference.
33605846	1	9	theme	disease	262:268	arg1	burden					270:275	the overall pneumococcal disease burden	237:275	the overall pneumococcal disease burden	237:275	The worldwide implementation of pneumococcal conjugate vaccines (PCVs) in children has reduced the overall pneumococcal disease burden.
33605846	9	10	theme	disease	1524:1530	arg1	burden					1532:1537	a similar residual overall disease burden	1497:1537	a similar residual overall disease burden with both vaccines	1497:1556	This results in a similar residual overall disease burden with both vaccines.
33605846	8	11	with	replacement	1370:1380	arg1	program					1430:1436	a PCV13 program	1422:1436	a PCV13 program	1422:1436	While protection against 19A is higher for PCV13 than PHiD-CV, replacement by non-PCV13 serotypes in settings with a PCV13 program appears to compensate for this difference.
33605846	5	12	theme	vaccine	940:946	arg1	serotypes					948:956	vaccine serotypes	940:956	vaccine serotypes	940:956	Both vaccines confer similarly high protection against disease caused by vaccine serotypes and lead to a partial replacement by non-vaccine serotypes.
33605846	9	13	theme	residual	1507:1514	arg1	burden					1532:1537	a similar residual overall disease burden	1497:1537	a similar residual overall disease burden with both vaccines	1497:1556	This results in a similar residual overall disease burden with both vaccines.
33605846	5	14	theme	high	898:901	arg1	protection					903:912	similarly high protection	888:912	similarly high protection against disease caused by vaccine serotypes	888:956	Both vaccines confer similarly high protection against disease caused by vaccine serotypes and lead to a partial replacement by non-vaccine serotypes.
33605846	7	15	dep	controlled	1283:1292	arg1	19A					1294:1296	19A	1294:1296	has fully controlled 19A disease	1273:1304	PHiD-CV provides some cross-protection against disease caused by vaccine-related serotype 19A but neither vaccine has fully controlled 19A disease.
33605846	0	16	from	impact	45:50	arg1	disease					133:139	overall pneumococcal disease	112:139	overall pneumococcal disease	112:139	Exploring the evidence behind the comparable impact of the pneumococcal conjugate vaccines PHiD-CV and PCV13 on overall pneumococcal disease.
33605846	5	17	theme	partial	972:978	arg1	replacement					980:990	a partial replacement	970:990	a partial replacement by non-vaccine serotypes	970:1015	Both vaccines confer similarly high protection against disease caused by vaccine serotypes and lead to a partial replacement by non-vaccine serotypes.
33605846	1	18	theme	pneumococcal	174:185	arg1	vaccines					197:204	pneumococcal conjugate vaccines	174:204	pneumococcal conjugate vaccines (PCVs)	174:211	The worldwide implementation of pneumococcal conjugate vaccines (PCVs) in children has reduced the overall pneumococcal disease burden.
33605846	1	18	theme	pneumococcal	174:185	arg1	PCVs					207:210	PCVs	207:210	PCVs	207:210	The worldwide implementation of pneumococcal conjugate vaccines (PCVs) in children has reduced the overall pneumococcal disease burden.
33605846	3	19	theme	serotypes	537:545	arg1	19A					557:559	19A	557:559	19A	557:559	While these PCVs differ in serotype composition (PCV13 includes polysaccharides of serotypes 3, 6A and 19A; PHiD-CV does not), their impact on the overall pneumococcal disease burden in children is comparable.
33605846	3	19	theme	serotypes	537:545	arg1	6A					550:551	6A	550:551	6A	550:551	While these PCVs differ in serotype composition (PCV13 includes polysaccharides of serotypes 3, 6A and 19A; PHiD-CV does not), their impact on the overall pneumococcal disease burden in children is comparable.
33605846	3	19	theme	serotypes	537:545	arg1	polysaccharides					518:532	polysaccharides	518:532	polysaccharides of serotypes 3	518:547	While these PCVs differ in serotype composition (PCV13 includes polysaccharides of serotypes 3, 6A and 19A; PHiD-CV does not), their impact on the overall pneumococcal disease burden in children is comparable.
33605846	3	20	theme	serotype	481:488	arg1	composition					490:500	serotype composition	481:500	serotype composition (PCV13 includes polysaccharides of serotypes 3, 6A and 19A; PHiD-CV does not)	481:578	While these PCVs differ in serotype composition (PCV13 includes polysaccharides of serotypes 3, 6A and 19A; PHiD-CV does not), their impact on the overall pneumococcal disease burden in children is comparable.
33605846	1	21	theme	conjugate	187:195	arg1	vaccines					197:204	pneumococcal conjugate vaccines	174:204	pneumococcal conjugate vaccines (PCVs)	174:211	The worldwide implementation of pneumococcal conjugate vaccines (PCVs) in children has reduced the overall pneumococcal disease burden.
33605846	1	21	theme	conjugate	187:195	arg1	PCVs					207:210	PCVs	207:210	PCVs	207:210	The worldwide implementation of pneumococcal conjugate vaccines (PCVs) in children has reduced the overall pneumococcal disease burden.
33605846	4	22	theme	clinical	850:857	arg1	impact					859:864	a differential clinical impact	835:864	a differential clinical impact	835:864	This commentary summarizes the evidence of comparability between PHiD-CV and PCV13 and explores why differences in serotype composition may not necessarily translate into a differential clinical impact.
33605846	3	23	from	burden	630:635	arg1	children					640:647	children	640:647	children	640:647	While these PCVs differ in serotype composition (PCV13 includes polysaccharides of serotypes 3, 6A and 19A; PHiD-CV does not), their impact on the overall pneumococcal disease burden in children is comparable.
33605846	0	24	dep	vaccines	82:89	arg1	vaccines					82:89	the pneumococcal conjugate vaccines PHiD-CV and PCV13	55:107	the pneumococcal conjugate vaccines PHiD-CV and PCV13	55:107	Exploring the evidence behind the comparable impact of the pneumococcal conjugate vaccines PHiD-CV and PCV13 on overall pneumococcal disease.
33605846	0	24	dep	vaccines	82:89	arg1	PCV13					103:107	PCV13	103:107	PCV13	103:107	Exploring the evidence behind the comparable impact of the pneumococcal conjugate vaccines PHiD-CV and PCV13 on overall pneumococcal disease.
33605846	0	24	dep	vaccines	82:89	arg1	PHiD-CV					91:97	PHiD-CV	91:97	PHiD-CV	91:97	Exploring the evidence behind the comparable impact of the pneumococcal conjugate vaccines PHiD-CV and PCV13 on overall pneumococcal disease.
33605846	1	25	theme	vaccines	197:204	arg1	implementation					156:169	The worldwide implementation	142:169	The worldwide implementation of pneumococcal conjugate vaccines (PCVs) in children	142:223	The worldwide implementation of pneumococcal conjugate vaccines (PCVs) in children has reduced the overall pneumococcal disease burden.
33605846	4	26	theme	differential	837:848	arg1	impact					859:864	a differential clinical impact	835:864	a differential clinical impact	835:864	This commentary summarizes the evidence of comparability between PHiD-CV and PCV13 and explores why differences in serotype composition may not necessarily translate into a differential clinical impact.
33605846	2	27	theme	infant	312:317	arg1	vaccination					319:329	infant vaccination	312:329	infant vaccination	312:329	Two PCVs are widely available for infant vaccination: the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) and the 13-valent PCV (PCV13).
33605846	2	28	theme	conjugate	395:403	arg1	PHiD-CV					414:420	PHiD-CV	414:420	PHiD-CV	414:420	Two PCVs are widely available for infant vaccination: the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) and the 13-valent PCV (PCV13).
33605846	2	28	theme	conjugate	395:403	arg1	vaccine					405:411	the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine	332:411	the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV)	332:421	Two PCVs are widely available for infant vaccination: the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) and the 13-valent PCV (PCV13).
33605846	0	29	theme	pneumococcal	120:131	arg1	disease					133:139	overall pneumococcal disease	112:139	overall pneumococcal disease	112:139	Exploring the evidence behind the comparable impact of the pneumococcal conjugate vaccines PHiD-CV and PCV13 on overall pneumococcal disease.
33605846	8	30	theme	non-PCV13	1385:1393	arg1	serotypes					1395:1403	non-PCV13 serotypes	1385:1403	non-PCV13 serotypes	1385:1403	While protection against 19A is higher for PCV13 than PHiD-CV, replacement by non-PCV13 serotypes in settings with a PCV13 program appears to compensate for this difference.
33605846	2	31	theme	13-valent	431:439	arg1	PCV13					446:450	PCV13	446:450	PCV13	446:450	Two PCVs are widely available for infant vaccination: the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) and the 13-valent PCV (PCV13).
33605846	2	31	theme	13-valent	431:439	arg1	PCV					441:443	the 13-valent PCV	427:443	the 13-valent PCV (PCV13)	427:451	Two PCVs are widely available for infant vaccination: the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) and the 13-valent PCV (PCV13).
33605846	4	32	theme	comparability	707:719	arg1	evidence					695:702	the evidence	691:702	the evidence of comparability between PHiD-CV and PCV13	691:745	This commentary summarizes the evidence of comparability between PHiD-CV and PCV13 and explores why differences in serotype composition may not necessarily translate into a differential clinical impact.
33605846	0	33	theme	overall	112:118	arg1	disease					133:139	overall pneumococcal disease	112:139	overall pneumococcal disease	112:139	Exploring the evidence behind the comparable impact of the pneumococcal conjugate vaccines PHiD-CV and PCV13 on overall pneumococcal disease.
33605846	3	34	theme	pneumococcal	609:620	arg1	burden					630:635	the overall pneumococcal disease burden	597:635	the overall pneumococcal disease burden in children	597:647	While these PCVs differ in serotype composition (PCV13 includes polysaccharides of serotypes 3, 6A and 19A; PHiD-CV does not), their impact on the overall pneumococcal disease burden in children is comparable.
33605846	6	35	dep	included	1075:1082	arg1	not					1071:1073	not	1071:1073	not	1071:1073	PHiD-CV does not protect against serotype 3 disease (not included in the vaccine) and PCV13's effect on this serotype has been inconsistent.
33605846	9	36	theme	overall	1516:1522	arg1	burden					1532:1537	a similar residual overall disease burden	1497:1537	a similar residual overall disease burden with both vaccines	1497:1556	This results in a similar residual overall disease burden with both vaccines.
33605846	0	37	theme	comparable	34:43	arg1	impact					45:50	the comparable impact	30:50	the comparable impact of the pneumococcal conjugate vaccines PHiD-CV and PCV13 on overall pneumococcal disease	30:139	Exploring the evidence behind the comparable impact of the pneumococcal conjugate vaccines PHiD-CV and PCV13 on overall pneumococcal disease.
33605846	3	38	dep	serotypes	537:545	arg1	3					547:547	3	547:547	3	547:547	While these PCVs differ in serotype composition (PCV13 includes polysaccharides of serotypes 3, 6A and 19A; PHiD-CV does not), their impact on the overall pneumococcal disease burden in children is comparable.
33605846	3	39	theme	disease	622:628	arg1	burden					630:635	the overall pneumococcal disease burden	597:635	the overall pneumococcal disease burden in children	597:647	While these PCVs differ in serotype composition (PCV13 includes polysaccharides of serotypes 3, 6A and 19A; PHiD-CV does not), their impact on the overall pneumococcal disease burden in children is comparable.
33605846	6	40	theme	serotype	1051:1058	arg1	disease					1062:1068	serotype 3 disease	1051:1068	serotype 3 disease (not included in the vaccine)	1051:1098	PHiD-CV does not protect against serotype 3 disease (not included in the vaccine) and PCV13's effect on this serotype has been inconsistent.
33605846	3	41	theme	overall	601:607	arg1	burden					630:635	the overall pneumococcal disease burden	597:635	the overall pneumococcal disease burden in children	597:647	While these PCVs differ in serotype composition (PCV13 includes polysaccharides of serotypes 3, 6A and 19A; PHiD-CV does not), their impact on the overall pneumococcal disease burden in children is comparable.
33605846	2	42	theme	non-typeable	349:360	arg1	PHiD-CV					414:420	PHiD-CV	414:420	PHiD-CV	414:420	Two PCVs are widely available for infant vaccination: the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) and the 13-valent PCV (PCV13).
33605846	2	42	theme	non-typeable	349:360	arg1	vaccine					405:411	the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine	332:411	the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV)	332:421	Two PCVs are widely available for infant vaccination: the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) and the 13-valent PCV (PCV13).
33605846	6	43	dep	disease	1062:1068	arg1	included					1075:1082	included	1075:1082	included in the vaccine	1075:1097	PHiD-CV does not protect against serotype 3 disease (not included in the vaccine) and PCV13's effect on this serotype has been inconsistent.
33605846	7	44	theme	vaccine-related	1224:1238	arg1	serotype					1240:1247	vaccine-related serotype	1224:1247	vaccine-related serotype 19A	1224:1251	PHiD-CV provides some cross-protection against disease caused by vaccine-related serotype 19A but neither vaccine has fully controlled 19A disease.
33605846	2	45	theme	pneumococcal	336:347	arg1	PHiD-CV					414:420	PHiD-CV	414:420	PHiD-CV	414:420	Two PCVs are widely available for infant vaccination: the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) and the 13-valent PCV (PCV13).
33605846	2	45	theme	pneumococcal	336:347	arg1	vaccine					405:411	the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine	332:411	the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV)	332:421	Two PCVs are widely available for infant vaccination: the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) and the 13-valent PCV (PCV13).
33605846	8	46	theme	PCV13	1424:1428	arg1	program					1430:1436	a PCV13 program	1422:1436	a PCV13 program	1422:1436	While protection against 19A is higher for PCV13 than PHiD-CV, replacement by non-PCV13 serotypes in settings with a PCV13 program appears to compensate for this difference.
33605846	6	47	from	effect	1112:1117	arg1	serotype					1127:1134	this serotype	1122:1134	this serotype	1122:1134	PHiD-CV does not protect against serotype 3 disease (not included in the vaccine) and PCV13's effect on this serotype has been inconsistent.
33605846	9	48	theme	similar	1499:1505	arg1	burden					1532:1537	a similar residual overall disease burden	1497:1537	a similar residual overall disease burden with both vaccines	1497:1556	This results in a similar residual overall disease burden with both vaccines.
33605846	3	49	dep	composition	490:500	arg1	includes					509:516	includes	509:516	includes polysaccharides of serotypes 3, 6A and 19A	509:559	While these PCVs differ in serotype composition (PCV13 includes polysaccharides of serotypes 3, 6A and 19A; PHiD-CV does not), their impact on the overall pneumococcal disease burden in children is comparable.
33605846	5	50	theme	non-vaccine	995:1005	arg1	serotypes					1007:1015	non-vaccine serotypes	995:1015	non-vaccine serotypes	995:1015	Both vaccines confer similarly high protection against disease caused by vaccine serotypes and lead to a partial replacement by non-vaccine serotypes.
33605846	2	51	theme	protein	385:391	arg1	PHiD-CV					414:420	PHiD-CV	414:420	PHiD-CV	414:420	Two PCVs are widely available for infant vaccination: the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) and the 13-valent PCV (PCV13).
33605846	2	51	theme	protein	385:391	arg1	vaccine					405:411	the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine	332:411	the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV)	332:421	Two PCVs are widely available for infant vaccination: the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) and the 13-valent PCV (PCV13).
33605846	3	52	from	impact	587:592	arg1	burden					630:635	the overall pneumococcal disease burden	597:635	the overall pneumococcal disease burden in children	597:647	While these PCVs differ in serotype composition (PCV13 includes polysaccharides of serotypes 3, 6A and 19A; PHiD-CV does not), their impact on the overall pneumococcal disease burden in children is comparable.
33605846	0	53	theme	pneumococcal	59:70	arg1	vaccines					82:89	the pneumococcal conjugate vaccines PHiD-CV and PCV13	55:107	the pneumococcal conjugate vaccines PHiD-CV and PCV13	55:107	Exploring the evidence behind the comparable impact of the pneumococcal conjugate vaccines PHiD-CV and PCV13 on overall pneumococcal disease.
33605846	0	53	theme	pneumococcal	59:70	arg1	PCV13					103:107	PCV13	103:107	PCV13	103:107	Exploring the evidence behind the comparable impact of the pneumococcal conjugate vaccines PHiD-CV and PCV13 on overall pneumococcal disease.
33605846	0	53	theme	pneumococcal	59:70	arg1	PHiD-CV					91:97	PHiD-CV	91:97	PHiD-CV	91:97	Exploring the evidence behind the comparable impact of the pneumococcal conjugate vaccines PHiD-CV and PCV13 on overall pneumococcal disease.
33605846	2	54	theme	D	393:393	arg1	PHiD-CV					414:420	PHiD-CV	414:420	PHiD-CV	414:420	Two PCVs are widely available for infant vaccination: the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) and the 13-valent PCV (PCV13).
33605846	2	54	theme	D	393:393	arg1	vaccine					405:411	the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine	332:411	the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV)	332:421	Two PCVs are widely available for infant vaccination: the pneumococcal non-typeable Haemophilus influenzae protein D conjugate vaccine (PHiD-CV) and the 13-valent PCV (PCV13).
33605846	4	55	theme	serotype	779:786	arg1	composition					788:798	serotype composition	779:798	serotype composition	779:798	This commentary summarizes the evidence of comparability between PHiD-CV and PCV13 and explores why differences in serotype composition may not necessarily translate into a differential clinical impact.
33605846	1	56	theme	worldwide	146:154	arg1	implementation					156:169	The worldwide implementation	142:169	The worldwide implementation of pneumococcal conjugate vaccines (PCVs) in children	142:223	The worldwide implementation of pneumococcal conjugate vaccines (PCVs) in children has reduced the overall pneumococcal disease burden.
33605846	1	57	theme	overall	241:247	arg1	burden					270:275	the overall pneumococcal disease burden	237:275	the overall pneumococcal disease burden	237:275	The worldwide implementation of pneumococcal conjugate vaccines (PCVs) in children has reduced the overall pneumococcal disease burden.
34625655	12	0	theme	brain	1717:1721	arg1	injury					1723:1728	inflammation-amplified HI brain injury	1691:1728	inflammation-amplified HI brain injury	1691:1728	IMPACT AZ treatment decreases sensorimotor impairment and severity of brain injury, and improves survival, after inflammation-amplified HI brain injury, and this can be achieved even with a 2 h delay in initiation.
34625655	10	1	theme	brain	1429:1433	arg1	injury					1435:1440	neonatal asphyxia brain injury	1411:1440	neonatal asphyxia brain injury	1411:1440	CONCLUSIONS Perinatal systemic infection is a common comorbidity of neonatal asphyxia brain injury and contributes to adverse outcomes.
34625655	13	2	theme	Gram-negative	1872:1884	arg1	infections					1904:1913	both Gram-negative and Gram-positive infections	1867:1913	both Gram-negative and Gram-positive infections	1867:1913	This neuroprotective benefit is seen in models of inflammation priming by both Gram-negative and Gram-positive infections.
34625655	2	3	theme	rat	320:322	arg1	injury					333:338	neonatal rat HI brain injury	311:338	neonatal rat HI brain injury	311:338	Azithromycin (AZ), an antibiotic with anti-inflammatory properties, improves sensorimotor function and reduces tissue damage after neonatal rat HI brain injury.
34625655	14	4	from	injury	2003:2008	arg1	rats					2022:2025	neonatal rats	2013:2025	neonatal rats	2013:2025	This extends our previous findings that AZ treatment is neuroprotective after HI brain injury in neonatal rats.
34625655	11	5	theme	neonatal	1552:1559	arg1	neuroprotection					1561:1575	neonatal neuroprotection	1552:1575	neonatal neuroprotection	1552:1575	These data support further evaluation of AZ as a candidate treatment for neonatal neuroprotection.
34625655	9	6	theme	longer	1318:1323	arg1	delay					1336:1340	longer initiation delay	1318:1340	longer initiation delay	1318:1340	Benefits increased with five- vs. three-dose AZ and declined with longer initiation delay.
34625655	12	7	theme	brain	1648:1652	arg1	injury					1654:1659	brain injury	1648:1659	brain injury	1648:1659	IMPACT AZ treatment decreases sensorimotor impairment and severity of brain injury, and improves survival, after inflammation-amplified HI brain injury, and this can be achieved even with a 2 h delay in initiation.
34625655	8	8	theme	LPS + HI	1128:1135	arg1	scores					1244:1249	composite scores	1234:1249	composite scores	1234:1249	RESULTS In both LPS + HI and PAM + HI models, AZ improved sensorimotor function, survival, brain tissue preservation, and composite scores.
34625655	8	8	theme	LPS + HI	1128:1135	arg1	function					1183:1190	AZ improved sensorimotor function	1158:1190	AZ improved sensorimotor function	1158:1190	RESULTS In both LPS + HI and PAM + HI models, AZ improved sensorimotor function, survival, brain tissue preservation, and composite scores.
34625655	8	8	theme	LPS + HI	1128:1135	arg1	models					1150:1155	both LPS + HI and PAM + HI models	1123:1155	both LPS + HI and PAM + HI models	1123:1155	RESULTS In both LPS + HI and PAM + HI models, AZ improved sensorimotor function, survival, brain tissue preservation, and composite scores.
34625655	8	8	theme	LPS + HI	1128:1135	arg1	preservation					1216:1227	brain tissue preservation	1203:1227	brain tissue preservation	1203:1227	RESULTS In both LPS + HI and PAM + HI models, AZ improved sensorimotor function, survival, brain tissue preservation, and composite scores.
34625655	8	8	theme	LPS + HI	1128:1135	arg1	survival					1193:1200	survival	1193:1200	survival	1193:1200	RESULTS In both LPS + HI and PAM + HI models, AZ improved sensorimotor function, survival, brain tissue preservation, and composite scores.
34625655	13	9	theme	inflammation	1843:1854	arg1	priming					1856:1862	inflammation priming	1843:1862	inflammation priming by both Gram-negative and Gram-positive infections	1843:1913	This neuroprotective benefit is seen in models of inflammation priming by both Gram-negative and Gram-positive infections.
34625655	6	10	theme	postnatal	920:928	arg1	day					930:932	postnatal day 35	920:935	postnatal day 35 outcomes	920:944	We compared postnatal day 35 outcomes in controls and groups treated with three or five AZ doses.
34625655	8	11	theme	PAM + HI	1141:1148	arg1	scores					1244:1249	composite scores	1234:1249	composite scores	1234:1249	RESULTS In both LPS + HI and PAM + HI models, AZ improved sensorimotor function, survival, brain tissue preservation, and composite scores.
34625655	8	11	theme	PAM + HI	1141:1148	arg1	function					1183:1190	AZ improved sensorimotor function	1158:1190	AZ improved sensorimotor function	1158:1190	RESULTS In both LPS + HI and PAM + HI models, AZ improved sensorimotor function, survival, brain tissue preservation, and composite scores.
34625655	8	11	theme	PAM + HI	1141:1148	arg1	models					1150:1155	both LPS + HI and PAM + HI models	1123:1155	both LPS + HI and PAM + HI models	1123:1155	RESULTS In both LPS + HI and PAM + HI models, AZ improved sensorimotor function, survival, brain tissue preservation, and composite scores.
34625655	8	11	theme	PAM + HI	1141:1148	arg1	preservation					1216:1227	brain tissue preservation	1203:1227	brain tissue preservation	1203:1227	RESULTS In both LPS + HI and PAM + HI models, AZ improved sensorimotor function, survival, brain tissue preservation, and composite scores.
34625655	8	11	theme	PAM + HI	1141:1148	arg1	survival					1193:1200	survival	1193:1200	survival	1193:1200	RESULTS In both LPS + HI and PAM + HI models, AZ improved sensorimotor function, survival, brain tissue preservation, and composite scores.
34625655	8	12	theme	composite	1234:1242	arg1	scores					1244:1249	composite scores	1234:1249	composite scores	1234:1249	RESULTS In both LPS + HI and PAM + HI models, AZ improved sensorimotor function, survival, brain tissue preservation, and composite scores.
34625655	8	12	theme	composite	1234:1242	arg1	models					1150:1155	both LPS + HI and PAM + HI models	1123:1155	both LPS + HI and PAM + HI models	1123:1155	RESULTS In both LPS + HI and PAM + HI models, AZ improved sensorimotor function, survival, brain tissue preservation, and composite scores.
34625655	2	13	theme	brain	327:331	arg1	injury					333:338	neonatal rat HI brain injury	311:338	neonatal rat HI brain injury	311:338	Azithromycin (AZ), an antibiotic with anti-inflammatory properties, improves sensorimotor function and reduces tissue damage after neonatal rat HI brain injury.
34625655	2	14	theme	sensorimotor	257:268	arg1	function					270:277	sensorimotor function	257:277	sensorimotor function	257:277	Azithromycin (AZ), an antibiotic with anti-inflammatory properties, improves sensorimotor function and reduces tissue damage after neonatal rat HI brain injury.
34625655	5	15	theme	%	815:815	arg1	hemisphere					829:838	%Intact right hemisphere	815:838	%Intact right hemisphere (brain damage)	815:853	Outcomes included contralateral forelimb function (forepaw placing; grip strength), survival, %Intact right hemisphere (brain damage), and a composite score incorporating these measures.
34625655	5	15	theme	%	815:815	arg1	damage					847:852	brain damage	841:852	brain damage	841:852	Outcomes included contralateral forelimb function (forepaw placing; grip strength), survival, %Intact right hemisphere (brain damage), and a composite score incorporating these measures.
34625655	4	16	theme	DESIGN/METHODS	481:494	arg1	rats					515:518	DESIGN/METHODS Seven-day-old (P7) rats	481:518	DESIGN/METHODS Seven-day-old (P7) rats	481:518	DESIGN/METHODS Seven-day-old (P7) rats received injections of toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI) in 8% oxygen.
34625655	4	16	theme	DESIGN/METHODS	481:494	arg1	P7					511:512	P7	511:512	P7	511:512	DESIGN/METHODS Seven-day-old (P7) rats received injections of toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI) in 8% oxygen.
34625655	8	17	theme	improved	1161:1168	arg1	function					1183:1190	AZ improved sensorimotor function	1158:1190	AZ improved sensorimotor function	1158:1190	RESULTS In both LPS + HI and PAM + HI models, AZ improved sensorimotor function, survival, brain tissue preservation, and composite scores.
34625655	8	17	theme	improved	1161:1168	arg1	models					1150:1155	both LPS + HI and PAM + HI models	1123:1155	both LPS + HI and PAM + HI models	1123:1155	RESULTS In both LPS + HI and PAM + HI models, AZ improved sensorimotor function, survival, brain tissue preservation, and composite scores.
34625655	14	18	theme	brain	1997:2001	arg1	injury					2003:2008	HI brain injury	1994:2008	HI brain injury in neonatal rats	1994:2025	This extends our previous findings that AZ treatment is neuroprotective after HI brain injury in neonatal rats.
34625655	1	19	theme	HI	162:163	arg1	injury					172:177	neonatal hypoxic-ischemic (HI) brain injury	135:177	neonatal hypoxic-ischemic (HI) brain injury	135:177	BACKGROUND Systemic inflammation amplifies neonatal hypoxic-ischemic (HI) brain injury.
34625655	2	20	theme	tissue	291:296	arg1	damage					298:303	tissue damage	291:303	tissue damage after neonatal rat HI brain injury	291:338	Azithromycin (AZ), an antibiotic with anti-inflammatory properties, improves sensorimotor function and reduces tissue damage after neonatal rat HI brain injury.
34625655	1	21	theme	brain	166:170	arg1	injury					172:177	neonatal hypoxic-ischemic (HI) brain injury	135:177	neonatal hypoxic-ischemic (HI) brain injury	135:177	BACKGROUND Systemic inflammation amplifies neonatal hypoxic-ischemic (HI) brain injury.
34625655	8	22	theme	brain	1203:1207	arg1	models					1150:1155	both LPS + HI and PAM + HI models	1123:1155	both LPS + HI and PAM + HI models	1123:1155	RESULTS In both LPS + HI and PAM + HI models, AZ improved sensorimotor function, survival, brain tissue preservation, and composite scores.
34625655	8	22	theme	brain	1203:1207	arg1	preservation					1216:1227	brain tissue preservation	1203:1227	brain tissue preservation	1203:1227	RESULTS In both LPS + HI and PAM + HI models, AZ improved sensorimotor function, survival, brain tissue preservation, and composite scores.
34625655	5	23	theme	forelimb	753:760	arg1	function					762:769	contralateral forelimb function	739:769	contralateral forelimb function (forepaw placing; grip strength)	739:802	Outcomes included contralateral forelimb function (forepaw placing; grip strength), survival, %Intact right hemisphere (brain damage), and a composite score incorporating these measures.
34625655	5	23	theme	forelimb	753:760	arg1	strength					794:801	forepaw placing; grip strength	772:801	forepaw placing; grip strength	772:801	Outcomes included contralateral forelimb function (forepaw placing; grip strength), survival, %Intact right hemisphere (brain damage), and a composite score incorporating these measures.
34625655	4	24	dep	agonists	562:569	arg1	LPS					591:593	LPS	591:593	LPS	591:593	DESIGN/METHODS Seven-day-old (P7) rats received injections of toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI) in 8% oxygen.
34625655	4	24	dep	agonists	562:569	arg1	Pam3Cys-Ser-					599:610	Pam3Cys-Ser-(Lys)4	599:616	Pam3Cys-Ser-(Lys)4	599:616	DESIGN/METHODS Seven-day-old (P7) rats received injections of toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI) in 8% oxygen.
34625655	4	24	dep	agonists	562:569	arg1	PAM					619:621	PAM	619:621	PAM	619:621	DESIGN/METHODS Seven-day-old (P7) rats received injections of toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI) in 8% oxygen.
34625655	4	24	dep	agonists	562:569	arg1	agonists					562:569	toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM)	543:622	toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI)	543:705	DESIGN/METHODS Seven-day-old (P7) rats received injections of toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI) in 8% oxygen.
34625655	4	24	dep	agonists	562:569	arg1	lipopolysaccharide					571:588	lipopolysaccharide	571:588	lipopolysaccharide (LPS)	571:594	DESIGN/METHODS Seven-day-old (P7) rats received injections of toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI) in 8% oxygen.
34625655	4	24	dep	agonists	562:569	arg1	Lys					612:614	Lys	612:614	Lys	612:614	DESIGN/METHODS Seven-day-old (P7) rats received injections of toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI) in 8% oxygen.
34625655	12	25	theme	sensorimotor	1608:1619	arg1	impairment					1621:1630	sensorimotor impairment	1608:1630	sensorimotor impairment	1608:1630	IMPACT AZ treatment decreases sensorimotor impairment and severity of brain injury, and improves survival, after inflammation-amplified HI brain injury, and this can be achieved even with a 2 h delay in initiation.
34625655	13	26	theme	neuroprotective	1798:1812	arg1	benefit					1814:1820	This neuroprotective benefit	1793:1820	This neuroprotective benefit	1793:1820	This neuroprotective benefit is seen in models of inflammation priming by both Gram-negative and Gram-positive infections.
34625655	14	27	theme	previous	1933:1940	arg1	findings					1942:1949	our previous findings that AZ treatment is neuroprotective after HI brain injury in neonatal rats	1929:2025	our previous findings that AZ treatment is neuroprotective after HI brain injury in neonatal rats	1929:2025	This extends our previous findings that AZ treatment is neuroprotective after HI brain injury in neonatal rats.
34625655	10	28	theme	systemic	1365:1372	arg1	infection					1374:1382	Perinatal systemic infection	1355:1382	Perinatal systemic infection	1355:1382	CONCLUSIONS Perinatal systemic infection is a common comorbidity of neonatal asphyxia brain injury and contributes to adverse outcomes.
34625655	10	28	theme	systemic	1365:1372	arg1	comorbidity					1396:1406	a common comorbidity	1387:1406	a common comorbidity of neonatal asphyxia brain injury	1387:1440	CONCLUSIONS Perinatal systemic infection is a common comorbidity of neonatal asphyxia brain injury and contributes to adverse outcomes.
34625655	3	29	theme	HI	464:465	arg1	injury					473:478	inflammation-amplified HI brain injury	441:478	inflammation-amplified HI brain injury	441:478	The objective of this study was to determine if AZ is neuroprotective in two neonatal rat models of inflammation-amplified HI brain injury.
34625655	3	30	from	neuroprotective	395:409	arg1	models					431:436	two neonatal rat models	414:436	two neonatal rat models of inflammation-amplified HI brain injury	414:478	The objective of this study was to determine if AZ is neuroprotective in two neonatal rat models of inflammation-amplified HI brain injury.
34625655	1	31	theme	Systemic	103:110	arg1	inflammation					112:123	BACKGROUND Systemic inflammation	92:123	BACKGROUND Systemic inflammation	92:123	BACKGROUND Systemic inflammation amplifies neonatal hypoxic-ischemic (HI) brain injury.
34625655	12	32	theme	IMPACT	1578:1583	arg1	treatment					1588:1596	IMPACT AZ treatment	1578:1596	IMPACT AZ treatment	1578:1596	IMPACT AZ treatment decreases sensorimotor impairment and severity of brain injury, and improves survival, after inflammation-amplified HI brain injury, and this can be achieved even with a 2 h delay in initiation.
34625655	4	33	theme	%	711:711	arg1	oxygen					713:718	8% oxygen	710:718	8% oxygen	710:718	DESIGN/METHODS Seven-day-old (P7) rats received injections of toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI) in 8% oxygen.
34625655	5	34	theme	grip	789:792	arg1	function					762:769	contralateral forelimb function	739:769	contralateral forelimb function (forepaw placing; grip strength)	739:802	Outcomes included contralateral forelimb function (forepaw placing; grip strength), survival, %Intact right hemisphere (brain damage), and a composite score incorporating these measures.
34625655	5	34	theme	grip	789:792	arg1	strength					794:801	forepaw placing; grip strength	772:801	forepaw placing; grip strength	772:801	Outcomes included contralateral forelimb function (forepaw placing; grip strength), survival, %Intact right hemisphere (brain damage), and a composite score incorporating these measures.
34625655	10	35	theme	common	1389:1394	arg1	infection					1374:1382	Perinatal systemic infection	1355:1382	Perinatal systemic infection	1355:1382	CONCLUSIONS Perinatal systemic infection is a common comorbidity of neonatal asphyxia brain injury and contributes to adverse outcomes.
34625655	10	35	theme	common	1389:1394	arg1	comorbidity					1396:1406	a common comorbidity	1387:1406	a common comorbidity of neonatal asphyxia brain injury	1387:1440	CONCLUSIONS Perinatal systemic infection is a common comorbidity of neonatal asphyxia brain injury and contributes to adverse outcomes.
34625655	0	36	theme	inflammation-amplified	21:42	arg1	injury					67:72	inflammation-amplified hypoxic-ischemic brain injury	21:72	inflammation-amplified hypoxic-ischemic brain injury in neonatal rats	21:89	Azithromycin reduces inflammation-amplified hypoxic-ischemic brain injury in neonatal rats.
34625655	1	37	theme	hypoxic-ischemic	144:159	arg1	injury					172:177	neonatal hypoxic-ischemic (HI) brain injury	135:177	neonatal hypoxic-ischemic (HI) brain injury	135:177	BACKGROUND Systemic inflammation amplifies neonatal hypoxic-ischemic (HI) brain injury.
34625655	3	38	theme	rat	427:429	arg1	models					431:436	two neonatal rat models	414:436	two neonatal rat models of inflammation-amplified HI brain injury	414:478	The objective of this study was to determine if AZ is neuroprotective in two neonatal rat models of inflammation-amplified HI brain injury.
34625655	3	39	theme	injury	473:478	arg1	models					431:436	two neonatal rat models	414:436	two neonatal rat models of inflammation-amplified HI brain injury	414:478	The objective of this study was to determine if AZ is neuroprotective in two neonatal rat models of inflammation-amplified HI brain injury.
34625655	0	40	theme	brain	61:65	arg1	injury					67:72	inflammation-amplified hypoxic-ischemic brain injury	21:72	inflammation-amplified hypoxic-ischemic brain injury in neonatal rats	21:89	Azithromycin reduces inflammation-amplified hypoxic-ischemic brain injury in neonatal rats.
34625655	7	41	theme	AZ	1062:1063	arg1	doses					1065:1069	the first (of five) AZ doses	1042:1069	the first (of five) AZ doses	1042:1069	Then, we compared P21 outcomes when the first (of five) AZ doses were administered 1, 2, or 4 h after HI.
34625655	4	42	theme	receptor	553:560	arg1	lipopolysaccharide					571:588	lipopolysaccharide	571:588	lipopolysaccharide (LPS)	571:594	DESIGN/METHODS Seven-day-old (P7) rats received injections of toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI) in 8% oxygen.
34625655	4	42	theme	receptor	553:560	arg1	agonists					562:569	toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM)	543:622	toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI)	543:705	DESIGN/METHODS Seven-day-old (P7) rats received injections of toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI) in 8% oxygen.
34625655	4	42	theme	receptor	553:560	arg1	Pam3Cys-Ser-					599:610	Pam3Cys-Ser-(Lys)4	599:616	Pam3Cys-Ser-(Lys)4	599:616	DESIGN/METHODS Seven-day-old (P7) rats received injections of toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI) in 8% oxygen.
34625655	10	43	theme	asphyxia	1420:1427	arg1	injury					1435:1440	neonatal asphyxia brain injury	1411:1440	neonatal asphyxia brain injury	1411:1440	CONCLUSIONS Perinatal systemic infection is a common comorbidity of neonatal asphyxia brain injury and contributes to adverse outcomes.
34625655	11	44	theme	further	1498:1504	arg1	treatment					1538:1546	a candidate treatment	1526:1546	a candidate treatment for neonatal neuroprotection	1526:1575	These data support further evaluation of AZ as a candidate treatment for neonatal neuroprotection.
34625655	11	44	theme	further	1498:1504	arg1	evaluation					1506:1515	further evaluation	1498:1515	further evaluation of AZ	1498:1521	These data support further evaluation of AZ as a candidate treatment for neonatal neuroprotection.
34625655	5	45	theme	placing	780:786	arg1	function					762:769	contralateral forelimb function	739:769	contralateral forelimb function (forepaw placing; grip strength)	739:802	Outcomes included contralateral forelimb function (forepaw placing; grip strength), survival, %Intact right hemisphere (brain damage), and a composite score incorporating these measures.
34625655	5	45	theme	placing	780:786	arg1	strength					794:801	forepaw placing; grip strength	772:801	forepaw placing; grip strength	772:801	Outcomes included contralateral forelimb function (forepaw placing; grip strength), survival, %Intact right hemisphere (brain damage), and a composite score incorporating these measures.
34625655	14	46	theme	AZ	1956:1957	arg1	treatment					1959:1967	AZ treatment	1956:1967	AZ treatment	1956:1967	This extends our previous findings that AZ treatment is neuroprotective after HI brain injury in neonatal rats.
34625655	2	47	theme	anti-inflammatory	218:234	arg1	properties					236:245	anti-inflammatory properties	218:245	anti-inflammatory properties	218:245	Azithromycin (AZ), an antibiotic with anti-inflammatory properties, improves sensorimotor function and reduces tissue damage after neonatal rat HI brain injury.
34625655	12	48	theme	HI	1714:1715	arg1	injury					1723:1728	inflammation-amplified HI brain injury	1691:1728	inflammation-amplified HI brain injury	1691:1728	IMPACT AZ treatment decreases sensorimotor impairment and severity of brain injury, and improves survival, after inflammation-amplified HI brain injury, and this can be achieved even with a 2 h delay in initiation.
34625655	5	49	theme	right	823:827	arg1	hemisphere					829:838	%Intact right hemisphere	815:838	%Intact right hemisphere (brain damage)	815:853	Outcomes included contralateral forelimb function (forepaw placing; grip strength), survival, %Intact right hemisphere (brain damage), and a composite score incorporating these measures.
34625655	5	49	theme	right	823:827	arg1	damage					847:852	brain damage	841:852	brain damage	841:852	Outcomes included contralateral forelimb function (forepaw placing; grip strength), survival, %Intact right hemisphere (brain damage), and a composite score incorporating these measures.
34625655	0	50	theme	neonatal	77:84	arg1	rats					86:89	neonatal rats	77:89	neonatal rats	77:89	Azithromycin reduces inflammation-amplified hypoxic-ischemic brain injury in neonatal rats.
34625655	2	51	with	antibiotic	202:211	arg1	properties					236:245	anti-inflammatory properties	218:245	anti-inflammatory properties	218:245	Azithromycin (AZ), an antibiotic with anti-inflammatory properties, improves sensorimotor function and reduces tissue damage after neonatal rat HI brain injury.
34625655	10	52	theme	injury	1435:1440	arg1	infection					1374:1382	Perinatal systemic infection	1355:1382	Perinatal systemic infection	1355:1382	CONCLUSIONS Perinatal systemic infection is a common comorbidity of neonatal asphyxia brain injury and contributes to adverse outcomes.
34625655	10	52	theme	injury	1435:1440	arg1	comorbidity					1396:1406	a common comorbidity	1387:1406	a common comorbidity of neonatal asphyxia brain injury	1387:1440	CONCLUSIONS Perinatal systemic infection is a common comorbidity of neonatal asphyxia brain injury and contributes to adverse outcomes.
34625655	7	53	theme	first	1046:1050	arg1	doses					1065:1069	the first (of five) AZ doses	1042:1069	the first (of five) AZ doses	1042:1069	Then, we compared P21 outcomes when the first (of five) AZ doses were administered 1, 2, or 4 h after HI.
34625655	11	54	theme	candidate	1528:1536	arg1	treatment					1538:1546	a candidate treatment	1526:1546	a candidate treatment for neonatal neuroprotection	1526:1575	These data support further evaluation of AZ as a candidate treatment for neonatal neuroprotection.
34625655	11	54	theme	candidate	1528:1536	arg1	evaluation					1506:1515	further evaluation	1498:1515	further evaluation of AZ	1498:1521	These data support further evaluation of AZ as a candidate treatment for neonatal neuroprotection.
34625655	4	55	from	injections	529:538	arg1	oxygen					713:718	8% oxygen	710:718	8% oxygen	710:718	DESIGN/METHODS Seven-day-old (P7) rats received injections of toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI) in 8% oxygen.
34625655	2	56	theme	HI	324:325	arg1	injury					333:338	neonatal rat HI brain injury	311:338	neonatal rat HI brain injury	311:338	Azithromycin (AZ), an antibiotic with anti-inflammatory properties, improves sensorimotor function and reduces tissue damage after neonatal rat HI brain injury.
34625655	10	57	theme	adverse	1461:1467	arg1	outcomes					1469:1476	adverse outcomes	1461:1476	adverse outcomes	1461:1476	CONCLUSIONS Perinatal systemic infection is a common comorbidity of neonatal asphyxia brain injury and contributes to adverse outcomes.
34625655	7	58	theme	P21	1024:1026	arg1	outcomes					1028:1035	P21 outcomes	1024:1035	P21 outcomes	1024:1035	Then, we compared P21 outcomes when the first (of five) AZ doses were administered 1, 2, or 4 h after HI.
34625655	2	59	theme	neonatal	311:318	arg1	injury					333:338	neonatal rat HI brain injury	311:338	neonatal rat HI brain injury	311:338	Azithromycin (AZ), an antibiotic with anti-inflammatory properties, improves sensorimotor function and reduces tissue damage after neonatal rat HI brain injury.
34625655	5	60	theme	Intact	816:821	arg1	hemisphere					829:838	%Intact right hemisphere	815:838	%Intact right hemisphere (brain damage)	815:853	Outcomes included contralateral forelimb function (forepaw placing; grip strength), survival, %Intact right hemisphere (brain damage), and a composite score incorporating these measures.
34625655	5	60	theme	Intact	816:821	arg1	damage					847:852	brain damage	841:852	brain damage	841:852	Outcomes included contralateral forelimb function (forepaw placing; grip strength), survival, %Intact right hemisphere (brain damage), and a composite score incorporating these measures.
34625655	12	61	theme	injury	1654:1659	arg1	severity					1636:1643	severity	1636:1643	severity of brain injury	1636:1659	IMPACT AZ treatment decreases sensorimotor impairment and severity of brain injury, and improves survival, after inflammation-amplified HI brain injury, and this can be achieved even with a 2 h delay in initiation.
34625655	12	61	theme	injury	1654:1659	arg1	impairment					1621:1630	sensorimotor impairment	1608:1630	sensorimotor impairment	1608:1630	IMPACT AZ treatment decreases sensorimotor impairment and severity of brain injury, and improves survival, after inflammation-amplified HI brain injury, and this can be achieved even with a 2 h delay in initiation.
34625655	9	62	theme	initiation	1325:1334	arg1	delay					1336:1340	longer initiation delay	1318:1340	longer initiation delay	1318:1340	Benefits increased with five- vs. three-dose AZ and declined with longer initiation delay.
34625655	6	63	theme	day	930:932	arg1	outcomes					937:944	postnatal day 35 outcomes	920:944	postnatal day 35 outcomes	920:944	We compared postnatal day 35 outcomes in controls and groups treated with three or five AZ doses.
34625655	7	64	dep	first	1046:1050	arg1	five					1056:1059	five	1056:1059	five	1056:1059	Then, we compared P21 outcomes when the first (of five) AZ doses were administered 1, 2, or 4 h after HI.
34625655	13	65	theme	priming	1856:1862	arg1	models					1833:1838	models	1833:1838	models of inflammation priming by both Gram-negative and Gram-positive infections	1833:1913	This neuroprotective benefit is seen in models of inflammation priming by both Gram-negative and Gram-positive infections.
34625655	4	66	theme	Seven-day-old	496:508	arg1	rats					515:518	DESIGN/METHODS Seven-day-old (P7) rats	481:518	DESIGN/METHODS Seven-day-old (P7) rats	481:518	DESIGN/METHODS Seven-day-old (P7) rats received injections of toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI) in 8% oxygen.
34625655	4	66	theme	Seven-day-old	496:508	arg1	P7					511:512	P7	511:512	P7	511:512	DESIGN/METHODS Seven-day-old (P7) rats received injections of toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI) in 8% oxygen.
34625655	4	67	theme	carotid	639:645	arg1	ligation					647:654	right carotid ligation	633:654	right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI)	633:705	DESIGN/METHODS Seven-day-old (P7) rats received injections of toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI) in 8% oxygen.
34625655	12	68	from	delay	1772:1776	arg1	initiation					1781:1790	initiation	1781:1790	initiation	1781:1790	IMPACT AZ treatment decreases sensorimotor impairment and severity of brain injury, and improves survival, after inflammation-amplified HI brain injury, and this can be achieved even with a 2 h delay in initiation.
34625655	8	69	theme	AZ	1158:1159	arg1	function					1183:1190	AZ improved sensorimotor function	1158:1190	AZ improved sensorimotor function	1158:1190	RESULTS In both LPS + HI and PAM + HI models, AZ improved sensorimotor function, survival, brain tissue preservation, and composite scores.
34625655	8	69	theme	AZ	1158:1159	arg1	models					1150:1155	both LPS + HI and PAM + HI models	1123:1155	both LPS + HI and PAM + HI models	1123:1155	RESULTS In both LPS + HI and PAM + HI models, AZ improved sensorimotor function, survival, brain tissue preservation, and composite scores.
34625655	8	70	from	RESULTS	1112:1118	arg1	scores					1244:1249	composite scores	1234:1249	composite scores	1234:1249	RESULTS In both LPS + HI and PAM + HI models, AZ improved sensorimotor function, survival, brain tissue preservation, and composite scores.
34625655	8	70	from	RESULTS	1112:1118	arg1	function					1183:1190	AZ improved sensorimotor function	1158:1190	AZ improved sensorimotor function	1158:1190	RESULTS In both LPS + HI and PAM + HI models, AZ improved sensorimotor function, survival, brain tissue preservation, and composite scores.
34625655	8	70	from	RESULTS	1112:1118	arg1	models					1150:1155	both LPS + HI and PAM + HI models	1123:1155	both LPS + HI and PAM + HI models	1123:1155	RESULTS In both LPS + HI and PAM + HI models, AZ improved sensorimotor function, survival, brain tissue preservation, and composite scores.
34625655	8	70	from	RESULTS	1112:1118	arg1	preservation					1216:1227	brain tissue preservation	1203:1227	brain tissue preservation	1203:1227	RESULTS In both LPS + HI and PAM + HI models, AZ improved sensorimotor function, survival, brain tissue preservation, and composite scores.
34625655	8	70	from	RESULTS	1112:1118	arg1	survival					1193:1200	survival	1193:1200	survival	1193:1200	RESULTS In both LPS + HI and PAM + HI models, AZ improved sensorimotor function, survival, brain tissue preservation, and composite scores.
34625655	5	71	theme	composite	862:870	arg1	score					872:876	a composite score	860:876	a composite score incorporating these measures	860:905	Outcomes included contralateral forelimb function (forepaw placing; grip strength), survival, %Intact right hemisphere (brain damage), and a composite score incorporating these measures.
34625655	8	72	theme	sensorimotor	1170:1181	arg1	function					1183:1190	AZ improved sensorimotor function	1158:1190	AZ improved sensorimotor function	1158:1190	RESULTS In both LPS + HI and PAM + HI models, AZ improved sensorimotor function, survival, brain tissue preservation, and composite scores.
34625655	8	72	theme	sensorimotor	1170:1181	arg1	models					1150:1155	both LPS + HI and PAM + HI models	1123:1155	both LPS + HI and PAM + HI models	1123:1155	RESULTS In both LPS + HI and PAM + HI models, AZ improved sensorimotor function, survival, brain tissue preservation, and composite scores.
34625655	4	73	theme	right	633:637	arg1	ligation					647:654	right carotid ligation	633:654	right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI)	633:705	DESIGN/METHODS Seven-day-old (P7) rats received injections of toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI) in 8% oxygen.
34625655	5	74	theme	brain	841:845	arg1	hemisphere					829:838	%Intact right hemisphere	815:838	%Intact right hemisphere (brain damage)	815:853	Outcomes included contralateral forelimb function (forepaw placing; grip strength), survival, %Intact right hemisphere (brain damage), and a composite score incorporating these measures.
34625655	5	74	theme	brain	841:845	arg1	damage					847:852	brain damage	841:852	brain damage	841:852	Outcomes included contralateral forelimb function (forepaw placing; grip strength), survival, %Intact right hemisphere (brain damage), and a composite score incorporating these measures.
34625655	3	75	theme	study	363:367	arg1	objective					345:353	The objective	341:353	The objective of this study	341:367	The objective of this study was to determine if AZ is neuroprotective in two neonatal rat models of inflammation-amplified HI brain injury.
34625655	5	76	theme	contralateral	739:751	arg1	function					762:769	contralateral forelimb function	739:769	contralateral forelimb function (forepaw placing; grip strength)	739:802	Outcomes included contralateral forelimb function (forepaw placing; grip strength), survival, %Intact right hemisphere (brain damage), and a composite score incorporating these measures.
34625655	5	76	theme	contralateral	739:751	arg1	strength					794:801	forepaw placing; grip strength	772:801	forepaw placing; grip strength	772:801	Outcomes included contralateral forelimb function (forepaw placing; grip strength), survival, %Intact right hemisphere (brain damage), and a composite score incorporating these measures.
34625655	12	77	theme	AZ	1585:1586	arg1	treatment					1588:1596	IMPACT AZ treatment	1578:1596	IMPACT AZ treatment	1578:1596	IMPACT AZ treatment decreases sensorimotor impairment and severity of brain injury, and improves survival, after inflammation-amplified HI brain injury, and this can be achieved even with a 2 h delay in initiation.
34625655	4	78	theme	toll-like	543:551	arg1	lipopolysaccharide					571:588	lipopolysaccharide	571:588	lipopolysaccharide (LPS)	571:594	DESIGN/METHODS Seven-day-old (P7) rats received injections of toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI) in 8% oxygen.
34625655	4	78	theme	toll-like	543:551	arg1	agonists					562:569	toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM)	543:622	toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI)	543:705	DESIGN/METHODS Seven-day-old (P7) rats received injections of toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI) in 8% oxygen.
34625655	4	78	theme	toll-like	543:551	arg1	Pam3Cys-Ser-					599:610	Pam3Cys-Ser-(Lys)4	599:616	Pam3Cys-Ser-(Lys)4	599:616	DESIGN/METHODS Seven-day-old (P7) rats received injections of toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI) in 8% oxygen.
34625655	3	79	from	models	431:436	arg1	neuroprotective					395:409	neuroprotective	395:409	neuroprotective	395:409	The objective of this study was to determine if AZ is neuroprotective in two neonatal rat models of inflammation-amplified HI brain injury.
34625655	13	80	theme	Gram-positive	1890:1902	arg1	infections					1904:1913	both Gram-negative and Gram-positive infections	1867:1913	both Gram-negative and Gram-positive infections	1867:1913	This neuroprotective benefit is seen in models of inflammation priming by both Gram-negative and Gram-positive infections.
34625655	7	81	dep	1	1089:1089	arg1	HI					1108:1109	HI	1108:1109	HI	1108:1109	Then, we compared P21 outcomes when the first (of five) AZ doses were administered 1, 2, or 4 h after HI.
34625655	8	82	theme	tissue	1209:1214	arg1	models					1150:1155	both LPS + HI and PAM + HI models	1123:1155	both LPS + HI and PAM + HI models	1123:1155	RESULTS In both LPS + HI and PAM + HI models, AZ improved sensorimotor function, survival, brain tissue preservation, and composite scores.
34625655	8	82	theme	tissue	1209:1214	arg1	preservation					1216:1227	brain tissue preservation	1203:1227	brain tissue preservation	1203:1227	RESULTS In both LPS + HI and PAM + HI models, AZ improved sensorimotor function, survival, brain tissue preservation, and composite scores.
34625655	1	83	theme	BACKGROUND	92:101	arg1	inflammation					112:123	BACKGROUND Systemic inflammation	92:123	BACKGROUND Systemic inflammation	92:123	BACKGROUND Systemic inflammation amplifies neonatal hypoxic-ischemic (HI) brain injury.
34625655	0	84	from	injury	67:72	arg1	rats					86:89	neonatal rats	77:89	neonatal rats	77:89	Azithromycin reduces inflammation-amplified hypoxic-ischemic brain injury in neonatal rats.
34625655	10	85	theme	Perinatal	1355:1363	arg1	infection					1374:1382	Perinatal systemic infection	1355:1382	Perinatal systemic infection	1355:1382	CONCLUSIONS Perinatal systemic infection is a common comorbidity of neonatal asphyxia brain injury and contributes to adverse outcomes.
34625655	10	85	theme	Perinatal	1355:1363	arg1	comorbidity					1396:1406	a common comorbidity	1387:1406	a common comorbidity of neonatal asphyxia brain injury	1387:1440	CONCLUSIONS Perinatal systemic infection is a common comorbidity of neonatal asphyxia brain injury and contributes to adverse outcomes.
34625655	3	86	theme	brain	467:471	arg1	injury					473:478	inflammation-amplified HI brain injury	441:478	inflammation-amplified HI brain injury	441:478	The objective of this study was to determine if AZ is neuroprotective in two neonatal rat models of inflammation-amplified HI brain injury.
34625655	10	87	dep	CONCLUSIONS	1343:1353	arg1	infection					1374:1382	Perinatal systemic infection	1355:1382	Perinatal systemic infection	1355:1382	CONCLUSIONS Perinatal systemic infection is a common comorbidity of neonatal asphyxia brain injury and contributes to adverse outcomes.
34625655	10	87	dep	CONCLUSIONS	1343:1353	arg1	contributes					1446:1456	contributes	1446:1456	contributes to adverse outcomes	1446:1476	CONCLUSIONS Perinatal systemic infection is a common comorbidity of neonatal asphyxia brain injury and contributes to adverse outcomes.
34625655	10	87	dep	CONCLUSIONS	1343:1353	arg1	comorbidity					1396:1406	a common comorbidity	1387:1406	a common comorbidity of neonatal asphyxia brain injury	1387:1440	CONCLUSIONS Perinatal systemic infection is a common comorbidity of neonatal asphyxia brain injury and contributes to adverse outcomes.
34625655	4	88	theme	8	710:710	arg1	%					711:711	%	711:711	%	711:711	DESIGN/METHODS Seven-day-old (P7) rats received injections of toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI) in 8% oxygen.
34625655	3	89	theme	inflammation-amplified	441:462	arg1	injury					473:478	inflammation-amplified HI brain injury	441:478	inflammation-amplified HI brain injury	441:478	The objective of this study was to determine if AZ is neuroprotective in two neonatal rat models of inflammation-amplified HI brain injury.
34625655	0	90	theme	hypoxic-ischemic	44:59	arg1	injury					67:72	inflammation-amplified hypoxic-ischemic brain injury	21:72	inflammation-amplified hypoxic-ischemic brain injury in neonatal rats	21:89	Azithromycin reduces inflammation-amplified hypoxic-ischemic brain injury in neonatal rats.
34625655	12	91	theme	2 h	1768:1770	arg1	delay					1772:1776	a 2 h delay	1766:1776	a 2 h delay in initiation	1766:1790	IMPACT AZ treatment decreases sensorimotor impairment and severity of brain injury, and improves survival, after inflammation-amplified HI brain injury, and this can be achieved even with a 2 h delay in initiation.
34625655	1	92	theme	neonatal	135:142	arg1	injury					172:177	neonatal hypoxic-ischemic (HI) brain injury	135:177	neonatal hypoxic-ischemic (HI) brain injury	135:177	BACKGROUND Systemic inflammation amplifies neonatal hypoxic-ischemic (HI) brain injury.
34625655	6	93	theme	AZ	996:997	arg1	doses					999:1003	three or five AZ doses	982:1003	three or five AZ doses	982:1003	We compared postnatal day 35 outcomes in controls and groups treated with three or five AZ doses.
34625655	14	94	theme	HI	1994:1995	arg1	injury					2003:2008	HI brain injury	1994:2008	HI brain injury in neonatal rats	1994:2025	This extends our previous findings that AZ treatment is neuroprotective after HI brain injury in neonatal rats.
34625655	4	95	theme	agonists	562:569	arg1	injections					529:538	injections	529:538	injections of toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI) in 8% oxygen	529:718	DESIGN/METHODS Seven-day-old (P7) rats received injections of toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI) in 8% oxygen.
34625655	5	96	theme	forepaw	772:778	arg1	function					762:769	contralateral forelimb function	739:769	contralateral forelimb function (forepaw placing; grip strength)	739:802	Outcomes included contralateral forelimb function (forepaw placing; grip strength), survival, %Intact right hemisphere (brain damage), and a composite score incorporating these measures.
34625655	5	96	theme	forepaw	772:778	arg1	strength					794:801	forepaw placing; grip strength	772:801	forepaw placing; grip strength	772:801	Outcomes included contralateral forelimb function (forepaw placing; grip strength), survival, %Intact right hemisphere (brain damage), and a composite score incorporating these measures.
34625655	3	97	theme	neonatal	418:425	arg1	models					431:436	two neonatal rat models	414:436	two neonatal rat models of inflammation-amplified HI brain injury	414:478	The objective of this study was to determine if AZ is neuroprotective in two neonatal rat models of inflammation-amplified HI brain injury.
34625655	10	98	theme	neonatal	1411:1418	arg1	injury					1435:1440	neonatal asphyxia brain injury	1411:1440	neonatal asphyxia brain injury	1411:1440	CONCLUSIONS Perinatal systemic infection is a common comorbidity of neonatal asphyxia brain injury and contributes to adverse outcomes.
34625655	14	99	theme	neonatal	2013:2020	arg1	rats					2022:2025	neonatal rats	2013:2025	neonatal rats	2013:2025	This extends our previous findings that AZ treatment is neuroprotective after HI brain injury in neonatal rats.
34625655	11	100	theme	AZ	1520:1521	arg1	treatment					1538:1546	a candidate treatment	1526:1546	a candidate treatment for neonatal neuroprotection	1526:1575	These data support further evaluation of AZ as a candidate treatment for neonatal neuroprotection.
34625655	11	100	theme	AZ	1520:1521	arg1	evaluation					1506:1515	further evaluation	1498:1515	further evaluation of AZ	1498:1521	These data support further evaluation of AZ as a candidate treatment for neonatal neuroprotection.
34625655	12	101	theme	inflammation-amplified	1691:1712	arg1	injury					1723:1728	inflammation-amplified HI brain injury	1691:1728	inflammation-amplified HI brain injury	1691:1728	IMPACT AZ treatment decreases sensorimotor impairment and severity of brain injury, and improves survival, after inflammation-amplified HI brain injury, and this can be achieved even with a 2 h delay in initiation.
34625655	4	102	theme	prior	624:628	arg1	lipopolysaccharide					571:588	lipopolysaccharide	571:588	lipopolysaccharide (LPS)	571:594	DESIGN/METHODS Seven-day-old (P7) rats received injections of toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI) in 8% oxygen.
34625655	4	102	theme	prior	624:628	arg1	agonists					562:569	toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM)	543:622	toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI)	543:705	DESIGN/METHODS Seven-day-old (P7) rats received injections of toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI) in 8% oxygen.
34625655	4	102	theme	prior	624:628	arg1	Pam3Cys-Ser-					599:610	Pam3Cys-Ser-(Lys)4	599:616	Pam3Cys-Ser-(Lys)4	599:616	DESIGN/METHODS Seven-day-old (P7) rats received injections of toll-like receptor agonists lipopolysaccharide (LPS) or Pam3Cys-Ser-(Lys)4 (PAM) prior to right carotid ligation followed by 50 min (LPS + HI) or 60 min (PAM + HI) in 8% oxygen.
32366032	4	0	theme	dietary	546:552	arg1	supplementation					565:579	dietary AhR ligand supplementation	546:579	dietary AhR ligand supplementation	546:579	Here, we investigated how dietary AhR ligand supplementation influences AhR target gene expression and intestinal microbiota composition.
32366032	6	1	from	expression	878:887	arg1	cells					910:914	intestinal immune cells	892:914	intestinal immune cells	892:914	AhRR expression in intestinal immune cells was mainly driven by dietary AhR ligands and was independent of microbial metabolites.
32366032	3	2	theme	important	461:469	arg1	sensor					471:476	an important sensor	458:476	an important sensor for environmental polyaromatic chemicals	458:517	I3C metabolites act as ligands of the aryl hydrocarbon receptor (AhR), an important sensor for environmental polyaromatic chemicals.
32366032	3	2	theme	important	461:469	arg1	receptor					442:449	the aryl hydrocarbon receptor	421:449	the aryl hydrocarbon receptor (AhR)	421:455	I3C metabolites act as ligands of the aryl hydrocarbon receptor (AhR), an important sensor for environmental polyaromatic chemicals.
32366032	1	3	theme	fiber	238:242	arg1	content					197:203	a high content	190:203	a high content of phytochemicals, vitamins, and fiber	190:242	A diet rich in vegetables and fruit is generally considered healthy because of a high content of phytochemicals, vitamins, and fiber.
32366032	4	4	theme	ligand	558:563	arg1	supplementation					565:579	dietary AhR ligand supplementation	546:579	dietary AhR ligand supplementation	546:579	Here, we investigated how dietary AhR ligand supplementation influences AhR target gene expression and intestinal microbiota composition.
32366032	2	5	theme	promising	352:360	arg1	benefits					377:384	promising diverse health benefits	352:384	a dietary supplement promising diverse health benefits	331:384	The phytochemical indole-3-carbinol (I3C), a derivative of glucobrassicin, is sold as a dietary supplement promising diverse health benefits.
32366032	2	6	theme	phytochemical	249:261	arg1	supplement					341:350	a dietary supplement promising diverse health benefits	331:384	a dietary supplement promising diverse health benefits	331:384	The phytochemical indole-3-carbinol (I3C), a derivative of glucobrassicin, is sold as a dietary supplement promising diverse health benefits.
32366032	2	6	theme	phytochemical	249:261	arg1	I3C					282:284	I3C	282:284	I3C	282:284	The phytochemical indole-3-carbinol (I3C), a derivative of glucobrassicin, is sold as a dietary supplement promising diverse health benefits.
32366032	2	6	theme	phytochemical	249:261	arg1	indole-3-carbinol					263:279	The phytochemical indole-3-carbinol	245:279	The phytochemical indole-3-carbinol (I3C)	245:285	The phytochemical indole-3-carbinol (I3C), a derivative of glucobrassicin, is sold as a dietary supplement promising diverse health benefits.
32366032	2	6	theme	phytochemical	249:261	arg1	derivative					290:299	a derivative	288:299	a derivative of glucobrassicin	288:317	The phytochemical indole-3-carbinol (I3C), a derivative of glucobrassicin, is sold as a dietary supplement promising diverse health benefits.
32366032	9	7	theme	AhR-dependent	1434:1446	arg1	alterations					1465:1475	both AhR-dependent and -independent alterations	1429:1475	both AhR-dependent and -independent alterations in the microbiome	1429:1493	Comparison of I3C-induced changes in microbiota composition using wild-type (WT), AhRR-deficient, and AhR-deficient mice revealed both AhR-dependent and -independent alterations in the microbiome.
32366032	5	8	used	used	671:674	arg2	we					668:669	we	668:669	we	668:669	For this, we used AhR repressor (AhRR)-reporter mice as a tool to study AhR activation in the intestine following dietary I3C-supplementation in comparison with AhR ligand-deprived diets, including a high fat diet.
32366032	7	9	dep	caused	1033:1038	arg1	whereas					1169:1175	whereas	1169:1175	whereas	1169:1175	A lack of dietary AhR ligands caused enhanced susceptibility to dextran sodium sulfate (DSS)-induced colitis and correlated with the expansion of Enterobacteriaceae, whereas Clostridiales, Muribaculaceae, and Rikenellaceae were strongly reduced.
32366032	9	10	theme	-independent	1452:1463	arg1	alterations					1465:1475	both AhR-dependent and -independent alterations	1429:1475	both AhR-dependent and -independent alterations in the microbiome	1429:1493	Comparison of I3C-induced changes in microbiota composition using wild-type (WT), AhRR-deficient, and AhR-deficient mice revealed both AhR-dependent and -independent alterations in the microbiome.
32366032	10	11	theme	Ahrr	1596:1599	arg1	expression					1601:1610	Ahrr expression	1596:1610	Ahrr expression in intestinal immune cells as well as microbiota composition	1596:1671	Overall, our study demonstrates that dietary AhR ligand supplementation has a profound influence on Ahrr expression in intestinal immune cells as well as microbiota composition.
32366032	5	12	theme	repressor	680:688	arg1	mice					706:709	AhR repressor (AhRR)-reporter mice	676:709	AhR repressor (AhRR)-reporter mice	676:709	For this, we used AhR repressor (AhRR)-reporter mice as a tool to study AhR activation in the intestine following dietary I3C-supplementation in comparison with AhR ligand-deprived diets, including a high fat diet.
32366032	5	12	theme	repressor	680:688	arg1	tool					716:719	a tool	714:719	a tool to study AhR activation in the intestine following dietary I3C-supplementation in comparison with AhR ligand-deprived diets, including a high fat diet	714:870	For this, we used AhR repressor (AhRR)-reporter mice as a tool to study AhR activation in the intestine following dietary I3C-supplementation in comparison with AhR ligand-deprived diets, including a high fat diet.
32366032	1	13	theme	high	192:195	arg1	content					197:203	a high content	190:203	a high content of phytochemicals, vitamins, and fiber	190:242	A diet rich in vegetables and fruit is generally considered healthy because of a high content of phytochemicals, vitamins, and fiber.
32366032	4	14	theme	intestinal	623:632	arg1	composition					645:655	intestinal microbiota composition	623:655	intestinal microbiota composition	623:655	Here, we investigated how dietary AhR ligand supplementation influences AhR target gene expression and intestinal microbiota composition.
32366032	7	15	theme	AhR	1021:1023	arg1	ligands					1025:1031	dietary AhR ligands	1013:1031	dietary AhR ligands	1013:1031	A lack of dietary AhR ligands caused enhanced susceptibility to dextran sodium sulfate (DSS)-induced colitis and correlated with the expansion of Enterobacteriaceae, whereas Clostridiales, Muribaculaceae, and Rikenellaceae were strongly reduced.
32366032	5	16	theme	AhR	819:821	arg1	diets					839:843	AhR ligand-deprived diets	819:843	AhR ligand-deprived diets	819:843	For this, we used AhR repressor (AhRR)-reporter mice as a tool to study AhR activation in the intestine following dietary I3C-supplementation in comparison with AhR ligand-deprived diets, including a high fat diet.
32366032	5	16	theme	AhR	819:821	arg1	diet					867:870	a high fat diet	856:870	a high fat diet	856:870	For this, we used AhR repressor (AhRR)-reporter mice as a tool to study AhR activation in the intestine following dietary I3C-supplementation in comparison with AhR ligand-deprived diets, including a high fat diet.
32366032	10	17	theme	immune	1626:1631	arg1	cells					1633:1637	intestinal immune cells	1615:1637	intestinal immune cells	1615:1637	Overall, our study demonstrates that dietary AhR ligand supplementation has a profound influence on Ahrr expression in intestinal immune cells as well as microbiota composition.
32366032	1	18	theme	rich	118:121	arg1	diet					113:116	A diet	111:116	A diet rich in vegetables and fruit	111:145	A diet rich in vegetables and fruit is generally considered healthy because of a high content of phytochemicals, vitamins, and fiber.
32366032	6	19	theme	intestinal	892:901	arg1	cells					910:914	intestinal immune cells	892:914	intestinal immune cells	892:914	AhRR expression in intestinal immune cells was mainly driven by dietary AhR ligands and was independent of microbial metabolites.
32366032	9	20	theme	AhR-deficient	1401:1413	arg1	mice					1415:1418	AhR-deficient mice	1401:1418	AhR-deficient mice	1401:1418	Comparison of I3C-induced changes in microbiota composition using wild-type (WT), AhRR-deficient, and AhR-deficient mice revealed both AhR-dependent and -independent alterations in the microbiome.
32366032	6	21	theme	microbial	980:988	arg1	metabolites					990:1000	microbial metabolites	980:1000	microbial metabolites	980:1000	AhRR expression in intestinal immune cells was mainly driven by dietary AhR ligands and was independent of microbial metabolites.
32366032	10	22	theme	AhR	1541:1543	arg1	supplementation					1552:1566	dietary AhR ligand supplementation	1533:1566	dietary AhR ligand supplementation	1533:1566	Overall, our study demonstrates that dietary AhR ligand supplementation has a profound influence on Ahrr expression in intestinal immune cells as well as microbiota composition.
32366032	0	23	theme	Immune	59:64	arg1	Cells					66:70	Intestinal Immune Cells	48:70	Intestinal Immune Cells	48:70	Dietary AhR Ligands Regulate AhRR Expression in Intestinal Immune Cells and Intestinal Microbiota Composition.
32366032	5	24	theme	high	858:861	arg1	diet					867:870	a high fat diet	856:870	a high fat diet	856:870	For this, we used AhR repressor (AhRR)-reporter mice as a tool to study AhR activation in the intestine following dietary I3C-supplementation in comparison with AhR ligand-deprived diets, including a high fat diet.
32366032	0	25	theme	Microbiota	87:96	arg1	Composition					98:108	Intestinal Microbiota Composition	76:108	Intestinal Microbiota Composition	76:108	Dietary AhR Ligands Regulate AhRR Expression in Intestinal Immune Cells and Intestinal Microbiota Composition.
32366032	6	26	theme	AhR	945:947	arg1	ligands					949:955	dietary AhR ligands	937:955	dietary AhR ligands	937:955	AhRR expression in intestinal immune cells was mainly driven by dietary AhR ligands and was independent of microbial metabolites.
32366032	2	27	theme	health	370:375	arg1	benefits					377:384	promising diverse health benefits	352:384	a dietary supplement promising diverse health benefits	331:384	The phytochemical indole-3-carbinol (I3C), a derivative of glucobrassicin, is sold as a dietary supplement promising diverse health benefits.
32366032	7	28	theme	Enterobacteriaceae	1149:1166	arg1	expansion					1136:1144	the expansion	1132:1144	the expansion of Enterobacteriaceae	1132:1166	A lack of dietary AhR ligands caused enhanced susceptibility to dextran sodium sulfate (DSS)-induced colitis and correlated with the expansion of Enterobacteriaceae, whereas Clostridiales, Muribaculaceae, and Rikenellaceae were strongly reduced.
32366032	9	29	from	composition	1347:1357	arg1	Comparison					1299:1308	Comparison	1299:1308	Comparison of I3C-induced changes in microbiota composition using wild-type (WT), AhRR-deficient, and AhR-deficient mice	1299:1418	Comparison of I3C-induced changes in microbiota composition using wild-type (WT), AhRR-deficient, and AhR-deficient mice revealed both AhR-dependent and -independent alterations in the microbiome.
32366032	10	30	contain	has	1568:1570	arg1	supplementation					1552:1566	dietary AhR ligand supplementation	1533:1566	dietary AhR ligand supplementation	1533:1566	Overall, our study demonstrates that dietary AhR ligand supplementation has a profound influence on Ahrr expression in intestinal immune cells as well as microbiota composition.
32366032	10	30	contain	has	1568:1570	arg2	influence					1583:1591	a profound influence	1572:1591	a profound influence	1572:1591	Overall, our study demonstrates that dietary AhR ligand supplementation has a profound influence on Ahrr expression in intestinal immune cells as well as microbiota composition.
32366032	9	31	dep	wild-type	1365:1373	arg1	WT					1376:1377	WT	1376:1377	WT	1376:1377	Comparison of I3C-induced changes in microbiota composition using wild-type (WT), AhRR-deficient, and AhR-deficient mice revealed both AhR-dependent and -independent alterations in the microbiome.
32366032	10	32	theme	microbiota	1650:1659	arg1	composition					1661:1671	microbiota composition	1650:1671	Ahrr expression in intestinal immune cells as well as microbiota composition	1596:1671	Overall, our study demonstrates that dietary AhR ligand supplementation has a profound influence on Ahrr expression in intestinal immune cells as well as microbiota composition.
32366032	0	33	theme	Dietary	0:6	arg1	Ligands					12:18	Dietary AhR Ligands	0:18	Dietary AhR Ligands	0:18	Dietary AhR Ligands Regulate AhRR Expression in Intestinal Immune Cells and Intestinal Microbiota Composition.
32366032	9	34	theme	changes	1325:1331	arg1	Comparison					1299:1308	Comparison	1299:1308	Comparison of I3C-induced changes in microbiota composition using wild-type (WT), AhRR-deficient, and AhR-deficient mice	1299:1418	Comparison of I3C-induced changes in microbiota composition using wild-type (WT), AhRR-deficient, and AhR-deficient mice revealed both AhR-dependent and -independent alterations in the microbiome.
32366032	3	35	theme	hydrocarbon	430:440	arg1	sensor					471:476	an important sensor	458:476	an important sensor for environmental polyaromatic chemicals	458:517	I3C metabolites act as ligands of the aryl hydrocarbon receptor (AhR), an important sensor for environmental polyaromatic chemicals.
32366032	3	35	theme	hydrocarbon	430:440	arg1	AhR					452:454	AhR	452:454	AhR	452:454	I3C metabolites act as ligands of the aryl hydrocarbon receptor (AhR), an important sensor for environmental polyaromatic chemicals.
32366032	3	35	theme	hydrocarbon	430:440	arg1	receptor					442:449	the aryl hydrocarbon receptor	421:449	the aryl hydrocarbon receptor (AhR)	421:455	I3C metabolites act as ligands of the aryl hydrocarbon receptor (AhR), an important sensor for environmental polyaromatic chemicals.
32366032	9	36	theme	microbiota	1336:1345	arg1	composition					1347:1357	microbiota composition	1336:1357	microbiota composition using wild-type (WT), AhRR-deficient, and AhR-deficient mice	1336:1418	Comparison of I3C-induced changes in microbiota composition using wild-type (WT), AhRR-deficient, and AhR-deficient mice revealed both AhR-dependent and -independent alterations in the microbiome.
32366032	3	37	theme	I3C	387:389	arg1	ligands					410:416	ligands	410:416	ligands of the aryl hydrocarbon receptor (AhR), an important sensor for environmental polyaromatic chemicals	410:517	I3C metabolites act as ligands of the aryl hydrocarbon receptor (AhR), an important sensor for environmental polyaromatic chemicals.
32366032	3	37	theme	I3C	387:389	arg1	metabolites					391:401	I3C metabolites	387:401	I3C metabolites	387:401	I3C metabolites act as ligands of the aryl hydrocarbon receptor (AhR), an important sensor for environmental polyaromatic chemicals.
32366032	0	38	theme	AhRR	29:32	arg1	Expression					34:43	AhRR Expression	29:43	AhRR Expression in Intestinal Immune Cells and Intestinal Microbiota Composition	29:108	Dietary AhR Ligands Regulate AhRR Expression in Intestinal Immune Cells and Intestinal Microbiota Composition.
32366032	10	39	theme	dietary	1533:1539	arg1	supplementation					1552:1566	dietary AhR ligand supplementation	1533:1566	dietary AhR ligand supplementation	1533:1566	Overall, our study demonstrates that dietary AhR ligand supplementation has a profound influence on Ahrr expression in intestinal immune cells as well as microbiota composition.
32366032	7	40	theme	-induced	1095:1102	arg1	colitis					1104:1110	dextran sodium sulfate (DSS)-induced colitis	1067:1110	dextran sodium sulfate (DSS)-induced colitis	1067:1110	A lack of dietary AhR ligands caused enhanced susceptibility to dextran sodium sulfate (DSS)-induced colitis and correlated with the expansion of Enterobacteriaceae, whereas Clostridiales, Muribaculaceae, and Rikenellaceae were strongly reduced.
32366032	9	41	from	alterations	1465:1475	arg1	microbiome					1484:1493	the microbiome	1480:1493	the microbiome	1480:1493	Comparison of I3C-induced changes in microbiota composition using wild-type (WT), AhRR-deficient, and AhR-deficient mice revealed both AhR-dependent and -independent alterations in the microbiome.
32366032	2	42	theme	glucobrassicin	304:317	arg1	indole-3-carbinol					263:279	The phytochemical indole-3-carbinol	245:279	The phytochemical indole-3-carbinol (I3C)	245:285	The phytochemical indole-3-carbinol (I3C), a derivative of glucobrassicin, is sold as a dietary supplement promising diverse health benefits.
32366032	2	42	theme	glucobrassicin	304:317	arg1	derivative					290:299	a derivative	288:299	a derivative of glucobrassicin	288:317	The phytochemical indole-3-carbinol (I3C), a derivative of glucobrassicin, is sold as a dietary supplement promising diverse health benefits.
32366032	1	43	theme	phytochemicals	208:221	arg1	content					197:203	a high content	190:203	a high content of phytochemicals, vitamins, and fiber	190:242	A diet rich in vegetables and fruit is generally considered healthy because of a high content of phytochemicals, vitamins, and fiber.
32366032	3	44	theme	polyaromatic	496:507	arg1	chemicals					509:517	environmental polyaromatic chemicals	482:517	environmental polyaromatic chemicals	482:517	I3C metabolites act as ligands of the aryl hydrocarbon receptor (AhR), an important sensor for environmental polyaromatic chemicals.
32366032	2	45	theme	dietary	333:339	arg1	supplement					341:350	a dietary supplement promising diverse health benefits	331:384	a dietary supplement promising diverse health benefits	331:384	The phytochemical indole-3-carbinol (I3C), a derivative of glucobrassicin, is sold as a dietary supplement promising diverse health benefits.
32366032	2	45	theme	dietary	333:339	arg1	indole-3-carbinol					263:279	The phytochemical indole-3-carbinol	245:279	The phytochemical indole-3-carbinol (I3C)	245:285	The phytochemical indole-3-carbinol (I3C), a derivative of glucobrassicin, is sold as a dietary supplement promising diverse health benefits.
32366032	1	46	theme	vitamins	224:231	arg1	content					197:203	a high content	190:203	a high content of phytochemicals, vitamins, and fiber	190:242	A diet rich in vegetables and fruit is generally considered healthy because of a high content of phytochemicals, vitamins, and fiber.
32366032	10	47	from	expression	1601:1610	arg1	cells					1633:1637	intestinal immune cells	1615:1637	intestinal immune cells	1615:1637	Overall, our study demonstrates that dietary AhR ligand supplementation has a profound influence on Ahrr expression in intestinal immune cells as well as microbiota composition.
32366032	4	48	theme	AhR	592:594	arg1	expression					608:617	AhR target gene expression	592:617	AhR target gene expression	592:617	Here, we investigated how dietary AhR ligand supplementation influences AhR target gene expression and intestinal microbiota composition.
32366032	10	49	theme	ligand	1545:1550	arg1	supplementation					1552:1566	dietary AhR ligand supplementation	1533:1566	dietary AhR ligand supplementation	1533:1566	Overall, our study demonstrates that dietary AhR ligand supplementation has a profound influence on Ahrr expression in intestinal immune cells as well as microbiota composition.
32366032	6	50	theme	AhRR	873:876	arg1	expression					878:887	AhRR expression	873:887	AhRR expression in intestinal immune cells	873:914	AhRR expression in intestinal immune cells was mainly driven by dietary AhR ligands and was independent of microbial metabolites.
32366032	4	51	theme	AhR	554:556	arg1	supplementation					565:579	dietary AhR ligand supplementation	546:579	dietary AhR ligand supplementation	546:579	Here, we investigated how dietary AhR ligand supplementation influences AhR target gene expression and intestinal microbiota composition.
32366032	10	52	theme	profound	1574:1581	arg1	influence					1583:1591	a profound influence	1572:1591	a profound influence	1572:1591	Overall, our study demonstrates that dietary AhR ligand supplementation has a profound influence on Ahrr expression in intestinal immune cells as well as microbiota composition.
32366032	3	53	theme	environmental	482:494	arg1	chemicals					509:517	environmental polyaromatic chemicals	482:517	environmental polyaromatic chemicals	482:517	I3C metabolites act as ligands of the aryl hydrocarbon receptor (AhR), an important sensor for environmental polyaromatic chemicals.
32366032	5	54	theme	ligand-deprived	823:837	arg1	diets					839:843	AhR ligand-deprived diets	819:843	AhR ligand-deprived diets	819:843	For this, we used AhR repressor (AhRR)-reporter mice as a tool to study AhR activation in the intestine following dietary I3C-supplementation in comparison with AhR ligand-deprived diets, including a high fat diet.
32366032	5	54	theme	ligand-deprived	823:837	arg1	diet					867:870	a high fat diet	856:870	a high fat diet	856:870	For this, we used AhR repressor (AhRR)-reporter mice as a tool to study AhR activation in the intestine following dietary I3C-supplementation in comparison with AhR ligand-deprived diets, including a high fat diet.
32366032	4	55	theme	microbiota	634:643	arg1	composition					645:655	intestinal microbiota composition	623:655	intestinal microbiota composition	623:655	Here, we investigated how dietary AhR ligand supplementation influences AhR target gene expression and intestinal microbiota composition.
32366032	10	56	theme	intestinal	1615:1624	arg1	cells					1633:1637	intestinal immune cells	1615:1637	intestinal immune cells	1615:1637	Overall, our study demonstrates that dietary AhR ligand supplementation has a profound influence on Ahrr expression in intestinal immune cells as well as microbiota composition.
32366032	7	57	theme	enhanced	1040:1047	arg1	susceptibility					1049:1062	enhanced susceptibility	1040:1062	enhanced susceptibility to dextran sodium sulfate (DSS)-induced colitis	1040:1110	A lack of dietary AhR ligands caused enhanced susceptibility to dextran sodium sulfate (DSS)-induced colitis and correlated with the expansion of Enterobacteriaceae, whereas Clostridiales, Muribaculaceae, and Rikenellaceae were strongly reduced.
32366032	6	58	theme	dietary	937:943	arg1	ligands					949:955	dietary AhR ligands	937:955	dietary AhR ligands	937:955	AhRR expression in intestinal immune cells was mainly driven by dietary AhR ligands and was independent of microbial metabolites.
32366032	5	59	theme	AhR	676:678	arg1	repressor					680:688	AhR repressor	676:688	AhR repressor (AhRR)-reporter mice	676:709	For this, we used AhR repressor (AhRR)-reporter mice as a tool to study AhR activation in the intestine following dietary I3C-supplementation in comparison with AhR ligand-deprived diets, including a high fat diet.
32366032	5	59	theme	AhR	676:678	arg1	AhRR					691:694	AhRR	691:694	AhRR	691:694	For this, we used AhR repressor (AhRR)-reporter mice as a tool to study AhR activation in the intestine following dietary I3C-supplementation in comparison with AhR ligand-deprived diets, including a high fat diet.
32366032	9	60	from	Comparison	1299:1308	arg1	composition					1347:1357	microbiota composition	1336:1357	microbiota composition using wild-type (WT), AhRR-deficient, and AhR-deficient mice	1336:1418	Comparison of I3C-induced changes in microbiota composition using wild-type (WT), AhRR-deficient, and AhR-deficient mice revealed both AhR-dependent and -independent alterations in the microbiome.
32366032	4	61	theme	gene	603:606	arg1	expression					608:617	AhR target gene expression	592:617	AhR target gene expression	592:617	Here, we investigated how dietary AhR ligand supplementation influences AhR target gene expression and intestinal microbiota composition.
32366032	7	62	theme	dietary	1013:1019	arg1	ligands					1025:1031	dietary AhR ligands	1013:1031	dietary AhR ligands	1013:1031	A lack of dietary AhR ligands caused enhanced susceptibility to dextran sodium sulfate (DSS)-induced colitis and correlated with the expansion of Enterobacteriaceae, whereas Clostridiales, Muribaculaceae, and Rikenellaceae were strongly reduced.
32366032	1	63	theme	content	197:203	arg1	healthy					171:177	healthy	171:177	healthy	171:177	A diet rich in vegetables and fruit is generally considered healthy because of a high content of phytochemicals, vitamins, and fiber.
32366032	8	64	theme	I3C	1249:1251	arg1	supplementation					1253:1267	I3C supplementation	1249:1267	I3C supplementation	1249:1267	I3C supplementation largely reverted this effect.
32366032	6	65	theme	immune	903:908	arg1	cells					910:914	intestinal immune cells	892:914	intestinal immune cells	892:914	AhRR expression in intestinal immune cells was mainly driven by dietary AhR ligands and was independent of microbial metabolites.
32366032	7	66	theme	ligands	1025:1031	arg1	lack					1005:1008	A lack	1003:1008	A lack of dietary AhR ligands	1003:1031	A lack of dietary AhR ligands caused enhanced susceptibility to dextran sodium sulfate (DSS)-induced colitis and correlated with the expansion of Enterobacteriaceae, whereas Clostridiales, Muribaculaceae, and Rikenellaceae were strongly reduced.
32366032	6	67	theme	metabolites	990:1000	arg1	independent					965:975	independent	965:975	independent	965:975	AhRR expression in intestinal immune cells was mainly driven by dietary AhR ligands and was independent of microbial metabolites.
32366032	5	68	theme	-reporter	696:704	arg1	mice					706:709	AhR repressor (AhRR)-reporter mice	676:709	AhR repressor (AhRR)-reporter mice	676:709	For this, we used AhR repressor (AhRR)-reporter mice as a tool to study AhR activation in the intestine following dietary I3C-supplementation in comparison with AhR ligand-deprived diets, including a high fat diet.
32366032	5	68	theme	-reporter	696:704	arg1	tool					716:719	a tool	714:719	a tool to study AhR activation in the intestine following dietary I3C-supplementation in comparison with AhR ligand-deprived diets, including a high fat diet	714:870	For this, we used AhR repressor (AhRR)-reporter mice as a tool to study AhR activation in the intestine following dietary I3C-supplementation in comparison with AhR ligand-deprived diets, including a high fat diet.
32366032	0	69	theme	Intestinal	76:85	arg1	Composition					98:108	Intestinal Microbiota Composition	76:108	Intestinal Microbiota Composition	76:108	Dietary AhR Ligands Regulate AhRR Expression in Intestinal Immune Cells and Intestinal Microbiota Composition.
32366032	5	70	theme	fat	863:865	arg1	diet					867:870	a high fat diet	856:870	a high fat diet	856:870	For this, we used AhR repressor (AhRR)-reporter mice as a tool to study AhR activation in the intestine following dietary I3C-supplementation in comparison with AhR ligand-deprived diets, including a high fat diet.
32366032	5	71	from	activation	734:743	arg1	intestine					752:760	the intestine	748:760	the intestine	748:760	For this, we used AhR repressor (AhRR)-reporter mice as a tool to study AhR activation in the intestine following dietary I3C-supplementation in comparison with AhR ligand-deprived diets, including a high fat diet.
32366032	5	72	from	I3C-supplementation	780:798	arg1	comparison					803:812	comparison	803:812	comparison with AhR ligand-deprived diets, including a high fat diet	803:870	For this, we used AhR repressor (AhRR)-reporter mice as a tool to study AhR activation in the intestine following dietary I3C-supplementation in comparison with AhR ligand-deprived diets, including a high fat diet.
32366032	1	73	from	fruit	141:145	arg1	rich					118:121	rich	118:121	rich	118:121	A diet rich in vegetables and fruit is generally considered healthy because of a high content of phytochemicals, vitamins, and fiber.
32366032	0	74	from	Expression	34:43	arg1	Composition					98:108	Intestinal Microbiota Composition	76:108	Intestinal Microbiota Composition	76:108	Dietary AhR Ligands Regulate AhRR Expression in Intestinal Immune Cells and Intestinal Microbiota Composition.
32366032	0	74	from	Expression	34:43	arg1	Cells					66:70	Intestinal Immune Cells	48:70	Intestinal Immune Cells	48:70	Dietary AhR Ligands Regulate AhRR Expression in Intestinal Immune Cells and Intestinal Microbiota Composition.
32366032	2	75	theme	diverse	362:368	arg1	benefits					377:384	promising diverse health benefits	352:384	a dietary supplement promising diverse health benefits	331:384	The phytochemical indole-3-carbinol (I3C), a derivative of glucobrassicin, is sold as a dietary supplement promising diverse health benefits.
32366032	0	76	theme	AhR	8:10	arg1	Ligands					12:18	Dietary AhR Ligands	0:18	Dietary AhR Ligands	0:18	Dietary AhR Ligands Regulate AhRR Expression in Intestinal Immune Cells and Intestinal Microbiota Composition.
32366032	9	77	theme	I3C-induced	1313:1323	arg1	changes					1325:1331	I3C-induced changes	1313:1331	I3C-induced changes in microbiota composition using wild-type (WT), AhRR-deficient, and AhR-deficient mice	1313:1418	Comparison of I3C-induced changes in microbiota composition using wild-type (WT), AhRR-deficient, and AhR-deficient mice revealed both AhR-dependent and -independent alterations in the microbiome.
32366032	9	78	from	changes	1325:1331	arg1	composition					1347:1357	microbiota composition	1336:1357	microbiota composition using wild-type (WT), AhRR-deficient, and AhR-deficient mice	1336:1418	Comparison of I3C-induced changes in microbiota composition using wild-type (WT), AhRR-deficient, and AhR-deficient mice revealed both AhR-dependent and -independent alterations in the microbiome.
32366032	1	79	from	vegetables	126:135	arg1	rich					118:121	rich	118:121	rich	118:121	A diet rich in vegetables and fruit is generally considered healthy because of a high content of phytochemicals, vitamins, and fiber.
32366032	5	80	theme	dietary	772:778	arg1	I3C-supplementation					780:798	dietary I3C-supplementation	772:798	dietary I3C-supplementation in comparison with AhR ligand-deprived diets, including a high fat diet	772:870	For this, we used AhR repressor (AhRR)-reporter mice as a tool to study AhR activation in the intestine following dietary I3C-supplementation in comparison with AhR ligand-deprived diets, including a high fat diet.
32366032	1	81	from	rich	118:121	arg1	fruit					141:145	fruit	141:145	fruit	141:145	A diet rich in vegetables and fruit is generally considered healthy because of a high content of phytochemicals, vitamins, and fiber.
32366032	1	81	from	rich	118:121	arg1	vegetables					126:135	vegetables	126:135	vegetables	126:135	A diet rich in vegetables and fruit is generally considered healthy because of a high content of phytochemicals, vitamins, and fiber.
32366032	3	82	theme	receptor	442:449	arg1	ligands					410:416	ligands	410:416	ligands of the aryl hydrocarbon receptor (AhR), an important sensor for environmental polyaromatic chemicals	410:517	I3C metabolites act as ligands of the aryl hydrocarbon receptor (AhR), an important sensor for environmental polyaromatic chemicals.
32366032	3	82	theme	receptor	442:449	arg1	metabolites					391:401	I3C metabolites	387:401	I3C metabolites	387:401	I3C metabolites act as ligands of the aryl hydrocarbon receptor (AhR), an important sensor for environmental polyaromatic chemicals.
32366032	5	83	theme	AhR	730:732	arg1	activation					734:743	AhR activation	730:743	AhR activation in the intestine following dietary I3C-supplementation in comparison with AhR ligand-deprived diets, including a high fat diet	730:870	For this, we used AhR repressor (AhRR)-reporter mice as a tool to study AhR activation in the intestine following dietary I3C-supplementation in comparison with AhR ligand-deprived diets, including a high fat diet.
32366032	5	84	with	comparison	803:812	arg1	diets					839:843	AhR ligand-deprived diets	819:843	AhR ligand-deprived diets	819:843	For this, we used AhR repressor (AhRR)-reporter mice as a tool to study AhR activation in the intestine following dietary I3C-supplementation in comparison with AhR ligand-deprived diets, including a high fat diet.
32366032	5	84	with	comparison	803:812	arg1	diet					867:870	a high fat diet	856:870	a high fat diet	856:870	For this, we used AhR repressor (AhRR)-reporter mice as a tool to study AhR activation in the intestine following dietary I3C-supplementation in comparison with AhR ligand-deprived diets, including a high fat diet.
32366032	3	85	theme	aryl	425:428	arg1	sensor					471:476	an important sensor	458:476	an important sensor for environmental polyaromatic chemicals	458:517	I3C metabolites act as ligands of the aryl hydrocarbon receptor (AhR), an important sensor for environmental polyaromatic chemicals.
32366032	3	85	theme	aryl	425:428	arg1	AhR					452:454	AhR	452:454	AhR	452:454	I3C metabolites act as ligands of the aryl hydrocarbon receptor (AhR), an important sensor for environmental polyaromatic chemicals.
32366032	3	85	theme	aryl	425:428	arg1	receptor					442:449	the aryl hydrocarbon receptor	421:449	the aryl hydrocarbon receptor (AhR)	421:455	I3C metabolites act as ligands of the aryl hydrocarbon receptor (AhR), an important sensor for environmental polyaromatic chemicals.
32366032	0	86	theme	Intestinal	48:57	arg1	Cells					66:70	Intestinal Immune Cells	48:70	Intestinal Immune Cells	48:70	Dietary AhR Ligands Regulate AhRR Expression in Intestinal Immune Cells and Intestinal Microbiota Composition.
32366032	10	87	from	composition	1661:1671	arg1	cells					1633:1637	intestinal immune cells	1615:1637	intestinal immune cells	1615:1637	Overall, our study demonstrates that dietary AhR ligand supplementation has a profound influence on Ahrr expression in intestinal immune cells as well as microbiota composition.
32366032	2	88	dep	supplement	341:350	arg1	benefits					377:384	promising diverse health benefits	352:384	a dietary supplement promising diverse health benefits	331:384	The phytochemical indole-3-carbinol (I3C), a derivative of glucobrassicin, is sold as a dietary supplement promising diverse health benefits.
32366032	4	89	theme	target	596:601	arg1	expression					608:617	AhR target gene expression	592:617	AhR target gene expression	592:617	Here, we investigated how dietary AhR ligand supplementation influences AhR target gene expression and intestinal microbiota composition.
32979412	18	0	theme	factor-kappa	1428:1439	arg1	NF-κB					1444:1448	NF-κB	1444:1448	NF-κB	1444:1448	Levels of nuclear factor-kappa B (NF-κB) in the nuclear of THP-1 cells and NF-κB, IκB-α, and FPR2 in the cytoplasm were tested by Western blot analysis.
32979412	18	0	theme	factor-kappa	1428:1439	arg1	B					1441:1441	nuclear factor-kappa B	1420:1441	nuclear factor-kappa B (NF-κB) in the nuclear of THP-1 cells	1420:1479	Levels of nuclear factor-kappa B (NF-κB) in the nuclear of THP-1 cells and NF-κB, IκB-α, and FPR2 in the cytoplasm were tested by Western blot analysis.
32979412	15	1	theme	substantial	1121:1131	arg1	composition					1133:1143	its substantial composition	1117:1143	its substantial composition	1117:1143	AIM OF THE STUDY This study was carried out to illustrate its pharmacological action and clarify its substantial composition.
32979412	27	2	theme	potential	2848:2856	arg1	mechanism					2858:2866	its potential mechanism	2844:2866	its potential mechanism	2844:2866	CONCLUSION Penyanling showed an anti-inflammatory effect on PID, and its potential mechanism involved suppressing NF-κB signal pathway and promoting the resolution of inflammation.
32979412	23	3	theme	receptor	2335:2342	arg1	expression					2306:2315	the expression	2302:2315	the expression of formyl peptide receptor 2 (FPR2), which suggested its effect on enhancing the resolution of inflammation	2302:2423	Besides, it could promote the expression of formyl peptide receptor 2 (FPR2), which suggested its effect on enhancing the resolution of inflammation.
32979412	21	4	theme	PID	1835:1837	arg1	rats					1839:1842	PID rats	1835:1842	PID rats	1835:1842	RESULTS The anti-inflammatory effects of Penyanling were observed on PID rats, such as suppressing the infiltrations of lymphocytes and neutrophils in the uterine tube, decreasing the release of interleukin (IL)-1β, IL-6, IL-8, and monocyte chemotactic protein (MCP)-1, and promoting the production of lipoxin A4 (LXA4).
32979412	16	5	theme	anti-inflammatory	1172:1188	arg1	effects					1190:1196	The anti-inflammatory effects	1168:1196	The anti-inflammatory effects	1168:1196	MATERIALS AND METHODS The anti-inflammatory effects of Penyanling were studied on a PID rat model and a lipopolysaccharides (LPS)-stimulated THP-1 cell line.
32979412	21	6	theme	IL-8	1988:1991	arg1	release					1950:1956	the release	1946:1956	the release of interleukin (IL)-1β, IL-6, IL-8, and monocyte chemotactic protein (MCP)-1	1946:2033	RESULTS The anti-inflammatory effects of Penyanling were observed on PID rats, such as suppressing the infiltrations of lymphocytes and neutrophils in the uterine tube, decreasing the release of interleukin (IL)-1β, IL-6, IL-8, and monocyte chemotactic protein (MCP)-1, and promoting the production of lipoxin A4 (LXA4).
32979412	14	7	theme	pelvic	958:963	arg1	PID					987:989	PID	987:989	PID	987:989	It has been used on pelvic inflammatory disease (PID) for more than twenty years.
32979412	14	7	theme	pelvic	958:963	arg1	disease					978:984	pelvic inflammatory disease	958:984	pelvic inflammatory disease (PID)	958:990	It has been used on pelvic inflammatory disease (PID) for more than twenty years.
32979412	18	8	theme	Western	1540:1546	arg1	analysis					1553:1560	Western blot analysis	1540:1560	Western blot analysis	1540:1560	Levels of nuclear factor-kappa B (NF-κB) in the nuclear of THP-1 cells and NF-κB, IκB-α, and FPR2 in the cytoplasm were tested by Western blot analysis.
32979412	21	9	from	infiltrations	1869:1881	arg1	tube					1929:1932	the uterine tube	1917:1932	the uterine tube	1917:1932	RESULTS The anti-inflammatory effects of Penyanling were observed on PID rats, such as suppressing the infiltrations of lymphocytes and neutrophils in the uterine tube, decreasing the release of interleukin (IL)-1β, IL-6, IL-8, and monocyte chemotactic protein (MCP)-1, and promoting the production of lipoxin A4 (LXA4).
32979412	17	10	from	levels	1329:1334	arg1	tube					1375:1378	the uterine tube	1363:1378	the uterine tube of the PID rat	1363:1393	Histological changes and levels of inflammatory factors in the uterine tube of the PID rat were examined.
32979412	11	11	dep	Scabiosaefolia	837:850	arg1	PS					853:854	PS	853:854	PS	853:854	, and Patrinia Scabiosaefolia (PS, from Patrinia scabiosaefolia Fisch.
32979412	17	12	from	changes	1317:1323	arg1	tube					1375:1378	the uterine tube	1363:1378	the uterine tube of the PID rat	1363:1393	Histological changes and levels of inflammatory factors in the uterine tube of the PID rat were examined.
32979412	5	13	dep	LR	463:464	arg1	Kosterm					497:503	Kosterm	497:503	LR, from Lindera aggregata (Sims) Kosterm.	463:504	, Linderae Radix (LR, from Lindera aggregata (Sims) Kosterm.)
32979412	2	14	dep	Angelicae	225:233	arg1	Oliv					279:282	Oliv	279:282	Oliv	279:282	, Angelicae Sinensis Radix (AS, from Angelica sinensis (Oliv.)
32979412	2	14	dep	Angelicae	225:233	arg1	Radix					244:248	Angelicae Sinensis Radix	225:248	Angelicae Sinensis Radix	225:248	, Angelicae Sinensis Radix (AS, from Angelica sinensis (Oliv.)
32979412	27	15	theme	anti-inflammatory	2807:2823	arg1	effect					2825:2830	an anti-inflammatory effect	2804:2830	an anti-inflammatory effect on PID	2804:2837	CONCLUSION Penyanling showed an anti-inflammatory effect on PID, and its potential mechanism involved suppressing NF-κB signal pathway and promoting the resolution of inflammation.
32979412	22	16	theme	effect	2268:2273	arg1	mechanism					2233:2241	the potential mechanism	2219:2241	the potential mechanism of its anti-inflammatory effect	2219:2273	On the other hand, Penyanling regulated the activity of NF-κB signal pathway on the LPS-stimulated THP-1 cell line, which suggested the potential mechanism of its anti-inflammatory effect.
32979412	10	17	from	Fisch	815:819	arg1	GR					784:785	GR	784:785	GR	784:785	, Glycyrrhizae Radix et Rhizoma (GR, from Glycyrrhiza uralensis Fisch.)
32979412	17	18	theme	factors	1352:1358	arg1	levels					1329:1334	levels	1329:1334	levels of inflammatory factors	1329:1358	Histological changes and levels of inflammatory factors in the uterine tube of the PID rat were examined.
32979412	17	18	theme	factors	1352:1358	arg1	changes					1317:1323	Histological changes	1304:1323	Histological changes	1304:1323	Histological changes and levels of inflammatory factors in the uterine tube of the PID rat were examined.
32979412	21	19	theme	neutrophils	1902:1912	arg1	infiltrations					1869:1881	the infiltrations	1865:1881	the infiltrations of lymphocytes and neutrophils in the uterine tube	1865:1932	RESULTS The anti-inflammatory effects of Penyanling were observed on PID rats, such as suppressing the infiltrations of lymphocytes and neutrophils in the uterine tube, decreasing the release of interleukin (IL)-1β, IL-6, IL-8, and monocyte chemotactic protein (MCP)-1, and promoting the production of lipoxin A4 (LXA4).
32979412	5	20	dep	Radix	456:460	arg1	Linderae					447:454	Linderae	447:454	Linderae	447:454	, Linderae Radix (LR, from Lindera aggregata (Sims) Kosterm.)
32979412	5	20	dep	Radix	456:460	arg1	LR					463:464	LR	463:464	LR	463:464	, Linderae Radix (LR, from Lindera aggregata (Sims) Kosterm.)
32979412	5	20	dep	Radix	456:460	arg1	Sims					491:494	Sims	491:494	Sims	491:494	, Linderae Radix (LR, from Lindera aggregata (Sims) Kosterm.)
32979412	22	21	theme	LPS-stimulated	2171:2184	arg1	line					2197:2200	the LPS-stimulated THP-1 cell line	2167:2200	the LPS-stimulated THP-1 cell line	2167:2200	On the other hand, Penyanling regulated the activity of NF-κB signal pathway on the LPS-stimulated THP-1 cell line, which suggested the potential mechanism of its anti-inflammatory effect.
32979412	6	22	dep	Paeoniae	508:515	arg1	PR					530:531	PR	530:531	PR	530:531	, Paeoniae Radix Rubra (PR, from Paeonia lactiflora Pall.)
32979412	6	22	dep	Paeoniae	508:515	arg1	Rubra					523:527	Paeoniae Radix Rubra	508:527	Paeoniae Radix Rubra	508:527	, Paeoniae Radix Rubra (PR, from Paeonia lactiflora Pall.)
32979412	19	23	theme	flight-mass	1651:1661	arg1	LC-Q-TOF-MS					1677:1687	LC-Q-TOF-MS	1677:1687	LC-Q-TOF-MS	1677:1687	Substances within Penyanling were scanned with liquid chromatography-quadrupole-time of flight-mass spectrometry (LC-Q-TOF-MS).
32979412	19	23	theme	flight-mass	1651:1661	arg1	spectrometry					1663:1674	flight-mass spectrometry	1651:1674	flight-mass spectrometry (LC-Q-TOF-MS)	1651:1688	Substances within Penyanling were scanned with liquid chromatography-quadrupole-time of flight-mass spectrometry (LC-Q-TOF-MS).
32979412	6	24	theme	Paeonia	539:545	arg1	Pall					558:561	Paeonia lactiflora Pall	539:561	Paeonia lactiflora Pall	539:561	, Paeoniae Radix Rubra (PR, from Paeonia lactiflora Pall.)
32979412	15	25	theme	STUDY	1031:1035	arg1	AIM					1020:1022	AIM	1020:1022	AIM OF THE STUDY	1020:1035	AIM OF THE STUDY This study was carried out to illustrate its pharmacological action and clarify its substantial composition.
32979412	7	26	dep	Rhizoma	576:582	arg1	Graebn					619:624	Graebn	619:624	Graebn	619:624	, Sparganii Rhizoma (SR, from Sparganium stoloniferum (Graebn.)
32979412	7	26	dep	Rhizoma	576:582	arg1	Sparganii					566:574	Sparganii	566:574	Sparganii	566:574	, Sparganii Rhizoma (SR, from Sparganium stoloniferum (Graebn.)
32979412	21	27	theme	lymphocytes	1886:1896	arg1	infiltrations					1869:1881	the infiltrations	1865:1881	the infiltrations of lymphocytes and neutrophils in the uterine tube	1865:1932	RESULTS The anti-inflammatory effects of Penyanling were observed on PID rats, such as suppressing the infiltrations of lymphocytes and neutrophils in the uterine tube, decreasing the release of interleukin (IL)-1β, IL-6, IL-8, and monocyte chemotactic protein (MCP)-1, and promoting the production of lipoxin A4 (LXA4).
32979412	24	28	theme	Seventy-six	2426:2436	arg1	substances					2438:2447	Seventy-six substances	2426:2447	Seventy-six substances	2426:2447	Seventy-six substances were identified by their accurate molecular weights, mass fragment patterns, retention times, and standards if available.
32979412	21	29	theme	A4	2076:2077	arg1	production					2054:2063	the production	2050:2063	the production of lipoxin A4 (LXA4)	2050:2084	RESULTS The anti-inflammatory effects of Penyanling were observed on PID rats, such as suppressing the infiltrations of lymphocytes and neutrophils in the uterine tube, decreasing the release of interleukin (IL)-1β, IL-6, IL-8, and monocyte chemotactic protein (MCP)-1, and promoting the production of lipoxin A4 (LXA4).
32979412	3	30	from	Bunge	363:367	arg1	SM					334:335	SM	334:335	SM	334:335	Diels), Salviae Miltiorrhizae Radix et Rhizoma (SM, from Salvia miltiorrhiza Bunge), Sargentodoxae Caulis (SC, from Sargentodoxa cuneata (Oliv.)
32979412	22	31	theme	signal	2149:2154	arg1	pathway					2156:2162	NF-κB signal pathway	2143:2162	NF-κB signal pathway	2143:2162	On the other hand, Penyanling regulated the activity of NF-κB signal pathway on the LPS-stimulated THP-1 cell line, which suggested the potential mechanism of its anti-inflammatory effect.
32979412	24	32	theme	retention	2526:2534	arg1	times					2536:2540	retention times	2526:2540	retention times	2526:2540	Seventy-six substances were identified by their accurate molecular weights, mass fragment patterns, retention times, and standards if available.
32979412	2	33	dep	Oliv	279:282	arg1	sinensis					269:276	Angelica sinensis	260:276	Angelica sinensis	260:276	, Angelicae Sinensis Radix (AS, from Angelica sinensis (Oliv.)
32979412	0	34	theme	inflammatory	90:101	arg1	disease					103:109	pelvic inflammatory disease	83:109	pelvic inflammatory disease	83:109	Anti-inflammatory effect of traditional Chinese medicine preparation Penyanling on pelvic inflammatory disease.
32979412	17	35	theme	Histological	1304:1315	arg1	changes					1317:1323	Histological changes	1304:1323	Histological changes	1304:1323	Histological changes and levels of inflammatory factors in the uterine tube of the PID rat were examined.
32979412	21	36	theme	interleukin	1961:1971	arg1	IL					1974:1975	interleukin (IL)-1β	1961:1979	interleukin (IL)-1β	1961:1979	RESULTS The anti-inflammatory effects of Penyanling were observed on PID rats, such as suppressing the infiltrations of lymphocytes and neutrophils in the uterine tube, decreasing the release of interleukin (IL)-1β, IL-6, IL-8, and monocyte chemotactic protein (MCP)-1, and promoting the production of lipoxin A4 (LXA4).
32979412	20	37	theme	total	1707:1711	arg1	flavonoids					1713:1722	total flavonoids	1707:1722	total flavonoids	1707:1722	The contents of total flavonoids, phenolics, and saponins were quantified.
32979412	27	38	theme	CONCLUSION	2775:2784	arg1	Penyanling					2786:2795	CONCLUSION Penyanling	2775:2795	CONCLUSION Penyanling	2775:2795	CONCLUSION Penyanling showed an anti-inflammatory effect on PID, and its potential mechanism involved suppressing NF-κB signal pathway and promoting the resolution of inflammation.
32979412	22	39	from	activity	2131:2138	arg1	line					2197:2200	the LPS-stimulated THP-1 cell line	2167:2200	the LPS-stimulated THP-1 cell line	2167:2200	On the other hand, Penyanling regulated the activity of NF-κB signal pathway on the LPS-stimulated THP-1 cell line, which suggested the potential mechanism of its anti-inflammatory effect.
32979412	16	40	theme	THP-1 cell	1287:1296	arg1	line					1298:1301	a lipopolysaccharides (LPS)-stimulated THP-1 cell line	1248:1301	a lipopolysaccharides (LPS)-stimulated THP-1 cell line	1248:1301	MATERIALS AND METHODS The anti-inflammatory effects of Penyanling were studied on a PID rat model and a lipopolysaccharides (LPS)-stimulated THP-1 cell line.
32979412	22	41	theme	anti-inflammatory	2250:2266	arg1	effect					2268:2273	its anti-inflammatory effect	2246:2273	its anti-inflammatory effect	2246:2273	On the other hand, Penyanling regulated the activity of NF-κB signal pathway on the LPS-stimulated THP-1 cell line, which suggested the potential mechanism of its anti-inflammatory effect.
32979412	17	42	theme	PID	1387:1389	arg1	rat					1391:1393	the PID rat	1383:1393	the PID rat	1383:1393	Histological changes and levels of inflammatory factors in the uterine tube of the PID rat were examined.
32979412	26	43	theme	total	2665:2669	arg1	flavonoids					2671:2680	total flavonoids	2665:2680	total flavonoids	2665:2680	The contents of total flavonoids, phenolics, and saponins within Penyanling were 0.186, 1.371, and 4.321 mg/mL, respectively.
32979412	21	44	located	observed	1823:1830	arg1	rats					1839:1842	PID rats	1835:1842	PID rats	1835:1842	RESULTS The anti-inflammatory effects of Penyanling were observed on PID rats, such as suppressing the infiltrations of lymphocytes and neutrophils in the uterine tube, decreasing the release of interleukin (IL)-1β, IL-6, IL-8, and monocyte chemotactic protein (MCP)-1, and promoting the production of lipoxin A4 (LXA4).
32979412	21	44	located	observed	1823:1830	arg2	RESULTS					1766:1772	RESULTS	1766:1772	RESULTS The anti-inflammatory effects of Penyanling	1766:1816	RESULTS The anti-inflammatory effects of Penyanling were observed on PID rats, such as suppressing the infiltrations of lymphocytes and neutrophils in the uterine tube, decreasing the release of interleukin (IL)-1β, IL-6, IL-8, and monocyte chemotactic protein (MCP)-1, and promoting the production of lipoxin A4 (LXA4).
32979412	15	45	theme	pharmacological	1082:1096	arg1	action					1098:1103	its pharmacological action	1078:1103	its pharmacological action	1078:1103	AIM OF THE STUDY This study was carried out to illustrate its pharmacological action and clarify its substantial composition.
32979412	0	46	theme	Anti-inflammatory	0:16	arg1	effect					18:23	Anti-inflammatory effect	0:23	Anti-inflammatory effect of traditional Chinese medicine preparation Penyanling on pelvic inflammatory disease.	0:110	Anti-inflammatory effect of traditional Chinese medicine preparation Penyanling on pelvic inflammatory disease.
32979412	1	47	theme	ETHNOPHARMACOLOGICAL	112:131	arg1	Penyanling					143:152	ETHNOPHARMACOLOGICAL RELEVANCE Penyanling	112:152	ETHNOPHARMACOLOGICAL RELEVANCE Penyanling	112:152	ETHNOPHARMACOLOGICAL RELEVANCE Penyanling is made up of Smilacis Glabrae Rhizoma (SG, from Smilar glabra Roxb.)
32979412	20	48	theme	saponins	1740:1747	arg1	contents					1695:1702	The contents	1691:1702	The contents of total flavonoids, phenolics, and saponins	1691:1747	The contents of total flavonoids, phenolics, and saponins were quantified.
32979412	22	49	theme	potential	2223:2231	arg1	mechanism					2233:2241	the potential mechanism	2219:2241	the potential mechanism of its anti-inflammatory effect	2219:2273	On the other hand, Penyanling regulated the activity of NF-κB signal pathway on the LPS-stimulated THP-1 cell line, which suggested the potential mechanism of its anti-inflammatory effect.
32979412	21	50	theme	protein	2019:2025	arg1	MCP					2028:2030	monocyte chemotactic protein (MCP)-1	1998:2033	monocyte chemotactic protein (MCP)-1	1998:2033	RESULTS The anti-inflammatory effects of Penyanling were observed on PID rats, such as suppressing the infiltrations of lymphocytes and neutrophils in the uterine tube, decreasing the release of interleukin (IL)-1β, IL-6, IL-8, and monocyte chemotactic protein (MCP)-1, and promoting the production of lipoxin A4 (LXA4).
32979412	0	51	theme	Chinese	40:46	arg1	medicine					48:55	traditional Chinese medicine preparation Penyanling	28:78	traditional Chinese medicine preparation Penyanling	28:78	Anti-inflammatory effect of traditional Chinese medicine preparation Penyanling on pelvic inflammatory disease.
32979412	21	52	theme	MCP	2028:2030	arg1	release					1950:1956	the release	1946:1956	the release of interleukin (IL)-1β, IL-6, IL-8, and monocyte chemotactic protein (MCP)-1	1946:2033	RESULTS The anti-inflammatory effects of Penyanling were observed on PID rats, such as suppressing the infiltrations of lymphocytes and neutrophils in the uterine tube, decreasing the release of interleukin (IL)-1β, IL-6, IL-8, and monocyte chemotactic protein (MCP)-1, and promoting the production of lipoxin A4 (LXA4).
32979412	0	53	theme	preparation	57:67	arg1	Penyanling					69:78	preparation Penyanling	57:78	traditional Chinese medicine preparation Penyanling	28:78	Anti-inflammatory effect of traditional Chinese medicine preparation Penyanling on pelvic inflammatory disease.
32979412	18	54	from	cytoplasm	1515:1523	arg1	Levels					1410:1415	Levels	1410:1415	Levels of nuclear factor-kappa B (NF-κB) in the nuclear of THP-1 cells and NF-κB, IκB-α, and FPR2 in the cytoplasm	1410:1523	Levels of nuclear factor-kappa B (NF-κB) in the nuclear of THP-1 cells and NF-κB, IκB-α, and FPR2 in the cytoplasm were tested by Western blot analysis.
32979412	27	55	theme	signal	2895:2900	arg1	pathway					2902:2908	NF-κB signal pathway	2889:2908	NF-κB signal pathway	2889:2908	CONCLUSION Penyanling showed an anti-inflammatory effect on PID, and its potential mechanism involved suppressing NF-κB signal pathway and promoting the resolution of inflammation.
32979412	16	56	theme	rat	1234:1236	arg1	model					1238:1242	a PID rat model	1228:1242	a PID rat model	1228:1242	MATERIALS AND METHODS The anti-inflammatory effects of Penyanling were studied on a PID rat model and a lipopolysaccharides (LPS)-stimulated THP-1 cell line.
32979412	21	57	theme	anti-inflammatory	1778:1794	arg1	effects					1796:1802	The anti-inflammatory effects	1774:1802	RESULTS The anti-inflammatory effects of Penyanling	1766:1816	RESULTS The anti-inflammatory effects of Penyanling were observed on PID rats, such as suppressing the infiltrations of lymphocytes and neutrophils in the uterine tube, decreasing the release of interleukin (IL)-1β, IL-6, IL-8, and monocyte chemotactic protein (MCP)-1, and promoting the production of lipoxin A4 (LXA4).
32979412	27	58	theme	inflammation	2942:2953	arg1	resolution					2928:2937	the resolution	2924:2937	the resolution of inflammation	2924:2953	CONCLUSION Penyanling showed an anti-inflammatory effect on PID, and its potential mechanism involved suppressing NF-κB signal pathway and promoting the resolution of inflammation.
32979412	24	59	theme	accurate	2474:2481	arg1	weights					2493:2499	their accurate molecular weights	2468:2499	their accurate molecular weights	2468:2499	Seventy-six substances were identified by their accurate molecular weights, mass fragment patterns, retention times, and standards if available.
32979412	2	60	theme	Angelica	260:267	arg1	sinensis					269:276	Angelica sinensis	260:276	Angelica sinensis	260:276	, Angelicae Sinensis Radix (AS, from Angelica sinensis (Oliv.)
32979412	10	61	dep	Radix	766:770	arg1	GR					784:785	GR	784:785	GR	784:785	, Glycyrrhizae Radix et Rhizoma (GR, from Glycyrrhiza uralensis Fisch.)
32979412	10	61	dep	Radix	766:770	arg1	Rhizoma					775:781	Glycyrrhizae Radix et Rhizoma	753:781	Glycyrrhizae Radix et Rhizoma (GR, from Glycyrrhiza uralensis Fisch.)	753:821	, Glycyrrhizae Radix et Rhizoma (GR, from Glycyrrhiza uralensis Fisch.)
32979412	9	62	dep	Rhizoma	651:657	arg1	CoR					660:662	CoR	660:662	CoR	660:662	, Corydalis Rhizoma (CoR, from Corydalis yanhusuo W. T. Wang), Cyperi Rhizoma (CyR, from Cyperus rotundus Linn.)
32979412	21	63	theme	monocyte	1998:2005	arg1	MCP					2028:2030	monocyte chemotactic protein (MCP)-1	1998:2033	monocyte chemotactic protein (MCP)-1	1998:2033	RESULTS The anti-inflammatory effects of Penyanling were observed on PID rats, such as suppressing the infiltrations of lymphocytes and neutrophils in the uterine tube, decreasing the release of interleukin (IL)-1β, IL-6, IL-8, and monocyte chemotactic protein (MCP)-1, and promoting the production of lipoxin A4 (LXA4).
32979412	18	64	from	nuclear	1458:1464	arg1	Levels					1410:1415	Levels	1410:1415	Levels of nuclear factor-kappa B (NF-κB) in the nuclear of THP-1 cells and NF-κB, IκB-α, and FPR2 in the cytoplasm	1410:1523	Levels of nuclear factor-kappa B (NF-κB) in the nuclear of THP-1 cells and NF-κB, IκB-α, and FPR2 in the cytoplasm were tested by Western blot analysis.
32979412	18	65	theme	B	1441:1441	arg1	Levels					1410:1415	Levels	1410:1415	Levels of nuclear factor-kappa B (NF-κB) in the nuclear of THP-1 cells and NF-κB, IκB-α, and FPR2 in the cytoplasm	1410:1523	Levels of nuclear factor-kappa B (NF-κB) in the nuclear of THP-1 cells and NF-κB, IκB-α, and FPR2 in the cytoplasm were tested by Western blot analysis.
32979412	24	66	theme	mass	2502:2505	arg1	patterns					2516:2523	mass fragment patterns	2502:2523	mass fragment patterns	2502:2523	Seventy-six substances were identified by their accurate molecular weights, mass fragment patterns, retention times, and standards if available.
32979412	16	67	dep	MATERIALS	1146:1154	arg1	effects					1190:1196	The anti-inflammatory effects	1168:1196	The anti-inflammatory effects	1168:1196	MATERIALS AND METHODS The anti-inflammatory effects of Penyanling were studied on a PID rat model and a lipopolysaccharides (LPS)-stimulated THP-1 cell line.
32979412	18	68	theme	nuclear	1420:1426	arg1	NF-κB					1444:1448	NF-κB	1444:1448	NF-κB	1444:1448	Levels of nuclear factor-kappa B (NF-κB) in the nuclear of THP-1 cells and NF-κB, IκB-α, and FPR2 in the cytoplasm were tested by Western blot analysis.
32979412	18	68	theme	nuclear	1420:1426	arg1	B					1441:1441	nuclear factor-kappa B	1420:1441	nuclear factor-kappa B (NF-κB) in the nuclear of THP-1 cells	1420:1479	Levels of nuclear factor-kappa B (NF-κB) in the nuclear of THP-1 cells and NF-κB, IκB-α, and FPR2 in the cytoplasm were tested by Western blot analysis.
32979412	22	69	theme	NF-κB	2143:2147	arg1	pathway					2156:2162	NF-κB signal pathway	2143:2162	NF-κB signal pathway	2143:2162	On the other hand, Penyanling regulated the activity of NF-κB signal pathway on the LPS-stimulated THP-1 cell line, which suggested the potential mechanism of its anti-inflammatory effect.
32979412	9	70	dep	Cyperus	728:734	arg1	rotundus					736:743	Cyperus rotundus Linn	728:748	Cyperus rotundus Linn	728:748	, Corydalis Rhizoma (CoR, from Corydalis yanhusuo W. T. Wang), Cyperi Rhizoma (CyR, from Cyperus rotundus Linn.)
32979412	9	70	dep	Cyperus	728:734	arg1	Linn					745:748	Linn	745:748	Linn	745:748	, Corydalis Rhizoma (CoR, from Corydalis yanhusuo W. T. Wang), Cyperi Rhizoma (CyR, from Cyperus rotundus Linn.)
32979412	18	71	from	NF-κB	1485:1489	arg1	cytoplasm					1515:1523	the cytoplasm	1511:1523	the cytoplasm	1511:1523	Levels of nuclear factor-kappa B (NF-κB) in the nuclear of THP-1 cells and NF-κB, IκB-α, and FPR2 in the cytoplasm were tested by Western blot analysis.
32979412	18	71	from	NF-κB	1485:1489	arg1	nuclear					1458:1464	nuclear	1458:1464	nuclear	1458:1464	Levels of nuclear factor-kappa B (NF-κB) in the nuclear of THP-1 cells and NF-κB, IκB-α, and FPR2 in the cytoplasm were tested by Western blot analysis.
32979412	27	72	theme	NF-κB	2889:2893	arg1	pathway					2902:2908	NF-κB signal pathway	2889:2908	NF-κB signal pathway	2889:2908	CONCLUSION Penyanling showed an anti-inflammatory effect on PID, and its potential mechanism involved suppressing NF-κB signal pathway and promoting the resolution of inflammation.
32979412	14	73	theme	inflammatory	965:976	arg1	PID					987:989	PID	987:989	PID	987:989	It has been used on pelvic inflammatory disease (PID) for more than twenty years.
32979412	14	73	theme	inflammatory	965:976	arg1	disease					978:984	pelvic inflammatory disease	958:984	pelvic inflammatory disease (PID)	958:990	It has been used on pelvic inflammatory disease (PID) for more than twenty years.
32979412	18	74	theme	blot	1548:1551	arg1	analysis					1553:1560	Western blot analysis	1540:1560	Western blot analysis	1540:1560	Levels of nuclear factor-kappa B (NF-κB) in the nuclear of THP-1 cells and NF-κB, IκB-α, and FPR2 in the cytoplasm were tested by Western blot analysis.
32979412	18	75	from	IκB-α	1492:1496	arg1	cytoplasm					1515:1523	the cytoplasm	1511:1523	the cytoplasm	1511:1523	Levels of nuclear factor-kappa B (NF-κB) in the nuclear of THP-1 cells and NF-κB, IκB-α, and FPR2 in the cytoplasm were tested by Western blot analysis.
32979412	18	75	from	IκB-α	1492:1496	arg1	nuclear					1458:1464	nuclear	1458:1464	nuclear	1458:1464	Levels of nuclear factor-kappa B (NF-κB) in the nuclear of THP-1 cells and NF-κB, IκB-α, and FPR2 in the cytoplasm were tested by Western blot analysis.
32979412	16	76	theme	Penyanling	1201:1210	arg1	METHODS					1160:1166	METHODS	1160:1166	METHODS	1160:1166	MATERIALS AND METHODS The anti-inflammatory effects of Penyanling were studied on a PID rat model and a lipopolysaccharides (LPS)-stimulated THP-1 cell line.
32979412	16	76	theme	Penyanling	1201:1210	arg1	MATERIALS					1146:1154	MATERIALS	1146:1154	MATERIALS	1146:1154	MATERIALS AND METHODS The anti-inflammatory effects of Penyanling were studied on a PID rat model and a lipopolysaccharides (LPS)-stimulated THP-1 cell line.
32979412	16	77	dep	-stimulated	1275:1285	arg1	lipopolysaccharides					1250:1268	lipopolysaccharides	1250:1268	lipopolysaccharides	1250:1268	MATERIALS AND METHODS The anti-inflammatory effects of Penyanling were studied on a PID rat model and a lipopolysaccharides (LPS)-stimulated THP-1 cell line.
32979412	6	78	from	Pall	558:561	arg1	PR					530:531	PR	530:531	PR	530:531	, Paeoniae Radix Rubra (PR, from Paeonia lactiflora Pall.)
32979412	21	79	theme	lipoxin	2068:2074	arg1	LXA4					2080:2083	LXA4	2080:2083	LXA4	2080:2083	RESULTS The anti-inflammatory effects of Penyanling were observed on PID rats, such as suppressing the infiltrations of lymphocytes and neutrophils in the uterine tube, decreasing the release of interleukin (IL)-1β, IL-6, IL-8, and monocyte chemotactic protein (MCP)-1, and promoting the production of lipoxin A4 (LXA4).
32979412	21	79	theme	lipoxin	2068:2074	arg1	A4					2076:2077	lipoxin A4	2068:2077	lipoxin A4 (LXA4)	2068:2084	RESULTS The anti-inflammatory effects of Penyanling were observed on PID rats, such as suppressing the infiltrations of lymphocytes and neutrophils in the uterine tube, decreasing the release of interleukin (IL)-1β, IL-6, IL-8, and monocyte chemotactic protein (MCP)-1, and promoting the production of lipoxin A4 (LXA4).
32979412	16	80	theme	-stimulated	1275:1285	arg1	line					1298:1301	a lipopolysaccharides (LPS)-stimulated THP-1 cell line	1248:1301	a lipopolysaccharides (LPS)-stimulated THP-1 cell line	1248:1301	MATERIALS AND METHODS The anti-inflammatory effects of Penyanling were studied on a PID rat model and a lipopolysaccharides (LPS)-stimulated THP-1 cell line.
32979412	18	81	from	B	1441:1441	arg1	cytoplasm					1515:1523	the cytoplasm	1511:1523	the cytoplasm	1511:1523	Levels of nuclear factor-kappa B (NF-κB) in the nuclear of THP-1 cells and NF-κB, IκB-α, and FPR2 in the cytoplasm were tested by Western blot analysis.
32979412	18	81	from	B	1441:1441	arg1	nuclear					1458:1464	nuclear	1458:1464	nuclear	1458:1464	Levels of nuclear factor-kappa B (NF-κB) in the nuclear of THP-1 cells and NF-κB, IκB-α, and FPR2 in the cytoplasm were tested by Western blot analysis.
32979412	23	82	theme	peptide	2327:2333	arg1	receptor					2335:2342	formyl peptide receptor 2	2320:2344	formyl peptide receptor 2 (FPR2)	2320:2351	Besides, it could promote the expression of formyl peptide receptor 2 (FPR2), which suggested its effect on enhancing the resolution of inflammation.
32979412	23	82	theme	peptide	2327:2333	arg1	FPR2					2347:2350	FPR2	2347:2350	FPR2	2347:2350	Besides, it could promote the expression of formyl peptide receptor 2 (FPR2), which suggested its effect on enhancing the resolution of inflammation.
32979412	15	83	dep	carried	1052:1058	arg1	AIM					1020:1022	AIM	1020:1022	AIM OF THE STUDY	1020:1035	AIM OF THE STUDY This study was carried out to illustrate its pharmacological action and clarify its substantial composition.
32979412	28	84	theme	main	2960:2963	arg1	substances					2965:2974	The main substances	2956:2974	The main substances within it	2956:2984	The main substances within it were flavonoids, phenolics, saponins, and alkaloids.
32979412	28	84	theme	main	2960:2963	arg1	flavonoids					2991:3000	flavonoids	2991:3000	flavonoids	2991:3000	The main substances within it were flavonoids, phenolics, saponins, and alkaloids.
32979412	19	85	theme	liquid	1610:1615	arg1	chromatography-quadrupole-time					1617:1646	liquid chromatography-quadrupole-time	1610:1646	liquid chromatography-quadrupole-time of flight-mass spectrometry (LC-Q-TOF-MS)	1610:1688	Substances within Penyanling were scanned with liquid chromatography-quadrupole-time of flight-mass spectrometry (LC-Q-TOF-MS).
32979412	22	86	theme	THP-1 cell	2186:2195	arg1	line					2197:2200	the LPS-stimulated THP-1 cell line	2167:2200	the LPS-stimulated THP-1 cell line	2167:2200	On the other hand, Penyanling regulated the activity of NF-κB signal pathway on the LPS-stimulated THP-1 cell line, which suggested the potential mechanism of its anti-inflammatory effect.
32979412	17	87	theme	uterine	1367:1373	arg1	tube					1375:1378	the uterine tube	1363:1378	the uterine tube of the PID rat	1363:1393	Histological changes and levels of inflammatory factors in the uterine tube of the PID rat were examined.
32979412	6	88	theme	lactiflora	547:556	arg1	Pall					558:561	Paeonia lactiflora Pall	539:561	Paeonia lactiflora Pall	539:561	, Paeoniae Radix Rubra (PR, from Paeonia lactiflora Pall.)
32979412	23	89	theme	formyl	2320:2325	arg1	receptor					2335:2342	formyl peptide receptor 2	2320:2344	formyl peptide receptor 2 (FPR2)	2320:2351	Besides, it could promote the expression of formyl peptide receptor 2 (FPR2), which suggested its effect on enhancing the resolution of inflammation.
32979412	23	89	theme	formyl	2320:2325	arg1	FPR2					2347:2350	FPR2	2347:2350	FPR2	2347:2350	Besides, it could promote the expression of formyl peptide receptor 2 (FPR2), which suggested its effect on enhancing the resolution of inflammation.
32979412	27	90	from	effect	2825:2830	arg1	PID					2835:2837	PID	2835:2837	PID	2835:2837	CONCLUSION Penyanling showed an anti-inflammatory effect on PID, and its potential mechanism involved suppressing NF-κB signal pathway and promoting the resolution of inflammation.
32979412	19	91	theme	spectrometry	1663:1674	arg1	chromatography-quadrupole-time					1617:1646	liquid chromatography-quadrupole-time	1610:1646	liquid chromatography-quadrupole-time of flight-mass spectrometry (LC-Q-TOF-MS)	1610:1688	Substances within Penyanling were scanned with liquid chromatography-quadrupole-time of flight-mass spectrometry (LC-Q-TOF-MS).
32979412	0	92	theme	pelvic	83:88	arg1	disease					103:109	pelvic inflammatory disease	83:109	pelvic inflammatory disease	83:109	Anti-inflammatory effect of traditional Chinese medicine preparation Penyanling on pelvic inflammatory disease.
32979412	22	93	theme	pathway	2156:2162	arg1	activity					2131:2138	the activity	2127:2138	the activity of NF-κB signal pathway on the LPS-stimulated THP-1 cell line, which suggested the potential mechanism of its anti-inflammatory effect	2127:2273	On the other hand, Penyanling regulated the activity of NF-κB signal pathway on the LPS-stimulated THP-1 cell line, which suggested the potential mechanism of its anti-inflammatory effect.
32979412	24	94	theme	fragment	2507:2514	arg1	patterns					2516:2523	mass fragment patterns	2502:2523	mass fragment patterns	2502:2523	Seventy-six substances were identified by their accurate molecular weights, mass fragment patterns, retention times, and standards if available.
32979412	17	95	theme	inflammatory	1339:1350	arg1	factors					1352:1358	inflammatory factors	1339:1358	inflammatory factors	1339:1358	Histological changes and levels of inflammatory factors in the uterine tube of the PID rat were examined.
32979412	26	96	theme	phenolics	2683:2691	arg1	contents					2653:2660	The contents	2649:2660	The contents of total flavonoids, phenolics, and saponins within Penyanling	2649:2723	The contents of total flavonoids, phenolics, and saponins within Penyanling were 0.186, 1.371, and 4.321 mg/mL, respectively.
32979412	26	96	theme	phenolics	2683:2691	arg1	0.186					2730:2734	0.186	2730:2734	0.186	2730:2734	The contents of total flavonoids, phenolics, and saponins within Penyanling were 0.186, 1.371, and 4.321 mg/mL, respectively.
32979412	0	97	from	effect	18:23	arg1	disease					103:109	pelvic inflammatory disease	83:109	pelvic inflammatory disease	83:109	Anti-inflammatory effect of traditional Chinese medicine preparation Penyanling on pelvic inflammatory disease.
32979412	13	98	theme	Chinese	915:921	arg1	Pharmacopoeia					923:935	Chinese Pharmacopoeia	915:935	Chinese Pharmacopoeia	915:935	recorded in Chinese Pharmacopoeia.
32979412	18	99	from	Levels	1410:1415	arg1	cytoplasm					1515:1523	the cytoplasm	1511:1523	the cytoplasm	1511:1523	Levels of nuclear factor-kappa B (NF-κB) in the nuclear of THP-1 cells and NF-κB, IκB-α, and FPR2 in the cytoplasm were tested by Western blot analysis.
32979412	18	99	from	Levels	1410:1415	arg1	nuclear					1458:1464	nuclear	1458:1464	nuclear	1458:1464	Levels of nuclear factor-kappa B (NF-κB) in the nuclear of THP-1 cells and NF-κB, IκB-α, and FPR2 in the cytoplasm were tested by Western blot analysis.
32979412	21	100	theme	uterine	1921:1927	arg1	tube					1929:1932	the uterine tube	1917:1932	the uterine tube	1917:1932	RESULTS The anti-inflammatory effects of Penyanling were observed on PID rats, such as suppressing the infiltrations of lymphocytes and neutrophils in the uterine tube, decreasing the release of interleukin (IL)-1β, IL-6, IL-8, and monocyte chemotactic protein (MCP)-1, and promoting the production of lipoxin A4 (LXA4).
32979412	26	101	theme	saponins	2698:2705	arg1	contents					2653:2660	The contents	2649:2660	The contents of total flavonoids, phenolics, and saponins within Penyanling	2649:2723	The contents of total flavonoids, phenolics, and saponins within Penyanling were 0.186, 1.371, and 4.321 mg/mL, respectively.
32979412	26	101	theme	saponins	2698:2705	arg1	0.186					2730:2734	0.186	2730:2734	0.186	2730:2734	The contents of total flavonoids, phenolics, and saponins within Penyanling were 0.186, 1.371, and 4.321 mg/mL, respectively.
32979412	14	102	used	used	950:953	arg2	It					938:939	It	938:939	It	938:939	It has been used on pelvic inflammatory disease (PID) for more than twenty years.
32979412	21	103	theme	IL-6	1982:1985	arg1	release					1950:1956	the release	1946:1956	the release of interleukin (IL)-1β, IL-6, IL-8, and monocyte chemotactic protein (MCP)-1	1946:2033	RESULTS The anti-inflammatory effects of Penyanling were observed on PID rats, such as suppressing the infiltrations of lymphocytes and neutrophils in the uterine tube, decreasing the release of interleukin (IL)-1β, IL-6, IL-8, and monocyte chemotactic protein (MCP)-1, and promoting the production of lipoxin A4 (LXA4).
32979412	26	104	theme	flavonoids	2671:2680	arg1	contents					2653:2660	The contents	2649:2660	The contents of total flavonoids, phenolics, and saponins within Penyanling	2649:2723	The contents of total flavonoids, phenolics, and saponins within Penyanling were 0.186, 1.371, and 4.321 mg/mL, respectively.
32979412	26	104	theme	flavonoids	2671:2680	arg1	0.186					2730:2734	0.186	2730:2734	0.186	2730:2734	The contents of total flavonoids, phenolics, and saponins within Penyanling were 0.186, 1.371, and 4.321 mg/mL, respectively.
32979412	1	105	dep	made	157:160	arg1	SG					194:195	SG	194:195	SG	194:195	ETHNOPHARMACOLOGICAL RELEVANCE Penyanling is made up of Smilacis Glabrae Rhizoma (SG, from Smilar glabra Roxb.)
32979412	18	106	theme	THP-1 cells	1469:1479	arg1	nuclear					1458:1464	nuclear	1458:1464	nuclear	1458:1464	Levels of nuclear factor-kappa B (NF-κB) in the nuclear of THP-1 cells and NF-κB, IκB-α, and FPR2 in the cytoplasm were tested by Western blot analysis.
32979412	21	107	dep	RESULTS	1766:1772	arg1	effects					1796:1802	The anti-inflammatory effects	1774:1802	RESULTS The anti-inflammatory effects of Penyanling	1766:1816	RESULTS The anti-inflammatory effects of Penyanling were observed on PID rats, such as suppressing the infiltrations of lymphocytes and neutrophils in the uterine tube, decreasing the release of interleukin (IL)-1β, IL-6, IL-8, and monocyte chemotactic protein (MCP)-1, and promoting the production of lipoxin A4 (LXA4).
32979412	17	108	theme	rat	1391:1393	arg1	tube					1375:1378	the uterine tube	1363:1378	the uterine tube of the PID rat	1363:1393	Histological changes and levels of inflammatory factors in the uterine tube of the PID rat were examined.
32979412	9	109	from	Wang	695:698	arg1	CoR					660:662	CoR	660:662	CoR	660:662	, Corydalis Rhizoma (CoR, from Corydalis yanhusuo W. T. Wang), Cyperi Rhizoma (CyR, from Cyperus rotundus Linn.)
32979412	21	110	theme	IL	1974:1975	arg1	release					1950:1956	the release	1946:1956	the release of interleukin (IL)-1β, IL-6, IL-8, and monocyte chemotactic protein (MCP)-1	1946:2033	RESULTS The anti-inflammatory effects of Penyanling were observed on PID rats, such as suppressing the infiltrations of lymphocytes and neutrophils in the uterine tube, decreasing the release of interleukin (IL)-1β, IL-6, IL-8, and monocyte chemotactic protein (MCP)-1, and promoting the production of lipoxin A4 (LXA4).
32979412	0	111	theme	traditional	28:38	arg1	medicine					48:55	traditional Chinese medicine preparation Penyanling	28:78	traditional Chinese medicine preparation Penyanling	28:78	Anti-inflammatory effect of traditional Chinese medicine preparation Penyanling on pelvic inflammatory disease.
32979412	22	112	theme	other	2094:2098	arg1	hand					2100:2103	the other hand	2090:2103	the other hand	2090:2103	On the other hand, Penyanling regulated the activity of NF-κB signal pathway on the LPS-stimulated THP-1 cell line, which suggested the potential mechanism of its anti-inflammatory effect.
32979412	1	113	theme	RELEVANCE	133:141	arg1	Penyanling					143:152	ETHNOPHARMACOLOGICAL RELEVANCE Penyanling	112:152	ETHNOPHARMACOLOGICAL RELEVANCE Penyanling	112:152	ETHNOPHARMACOLOGICAL RELEVANCE Penyanling is made up of Smilacis Glabrae Rhizoma (SG, from Smilar glabra Roxb.)
32979412	21	114	theme	Penyanling	1807:1816	arg1	effects					1796:1802	The anti-inflammatory effects	1774:1802	RESULTS The anti-inflammatory effects of Penyanling	1766:1816	RESULTS The anti-inflammatory effects of Penyanling were observed on PID rats, such as suppressing the infiltrations of lymphocytes and neutrophils in the uterine tube, decreasing the release of interleukin (IL)-1β, IL-6, IL-8, and monocyte chemotactic protein (MCP)-1, and promoting the production of lipoxin A4 (LXA4).
32979412	0	115	theme	medicine	48:55	arg1	effect					18:23	Anti-inflammatory effect	0:23	Anti-inflammatory effect of traditional Chinese medicine preparation Penyanling on pelvic inflammatory disease.	0:110	Anti-inflammatory effect of traditional Chinese medicine preparation Penyanling on pelvic inflammatory disease.
32979412	7	116	dep	Graebn	619:624	arg1	stoloniferum					605:616	stoloniferum	605:616	stoloniferum	605:616	, Sparganii Rhizoma (SR, from Sparganium stoloniferum (Graebn.)
32979412	7	116	dep	Graebn	619:624	arg1	SR					585:586	SR	585:586	SR	585:586	, Sparganii Rhizoma (SR, from Sparganium stoloniferum (Graebn.)
32979412	3	117	dep	Radix	316:320	arg1	Rhizoma					325:331	Salviae Miltiorrhizae Radix et Rhizoma	294:331	Salviae Miltiorrhizae Radix et Rhizoma (SM, from Salvia miltiorrhiza Bunge)	294:368	Diels), Salviae Miltiorrhizae Radix et Rhizoma (SM, from Salvia miltiorrhiza Bunge), Sargentodoxae Caulis (SC, from Sargentodoxa cuneata (Oliv.)
32979412	3	117	dep	Radix	316:320	arg1	SM					334:335	SM	334:335	SM	334:335	Diels), Salviae Miltiorrhizae Radix et Rhizoma (SM, from Salvia miltiorrhiza Bunge), Sargentodoxae Caulis (SC, from Sargentodoxa cuneata (Oliv.)
32979412	3	118	theme	Sargentodoxa	402:413	arg1	cuneata					415:421	Sargentodoxa cuneata	402:421	Sargentodoxa cuneata	402:421	Diels), Salviae Miltiorrhizae Radix et Rhizoma (SM, from Salvia miltiorrhiza Bunge), Sargentodoxae Caulis (SC, from Sargentodoxa cuneata (Oliv.)
32979412	18	119	theme	NF-κB	1485:1489	arg1	Levels					1410:1415	Levels	1410:1415	Levels of nuclear factor-kappa B (NF-κB) in the nuclear of THP-1 cells and NF-κB, IκB-α, and FPR2 in the cytoplasm	1410:1523	Levels of nuclear factor-kappa B (NF-κB) in the nuclear of THP-1 cells and NF-κB, IκB-α, and FPR2 in the cytoplasm were tested by Western blot analysis.
32979412	18	120	from	FPR2	1503:1506	arg1	cytoplasm					1515:1523	the cytoplasm	1511:1523	the cytoplasm	1511:1523	Levels of nuclear factor-kappa B (NF-κB) in the nuclear of THP-1 cells and NF-κB, IκB-α, and FPR2 in the cytoplasm were tested by Western blot analysis.
32979412	18	120	from	FPR2	1503:1506	arg1	nuclear					1458:1464	nuclear	1458:1464	nuclear	1458:1464	Levels of nuclear factor-kappa B (NF-κB) in the nuclear of THP-1 cells and NF-κB, IκB-α, and FPR2 in the cytoplasm were tested by Western blot analysis.
32979412	0	121	dep	medicine	48:55	arg1	Penyanling					69:78	preparation Penyanling	57:78	traditional Chinese medicine preparation Penyanling	28:78	Anti-inflammatory effect of traditional Chinese medicine preparation Penyanling on pelvic inflammatory disease.
32979412	20	122	theme	flavonoids	1713:1722	arg1	contents					1695:1702	The contents	1691:1702	The contents of total flavonoids, phenolics, and saponins	1691:1747	The contents of total flavonoids, phenolics, and saponins were quantified.
32979412	16	123	theme	PID	1230:1232	arg1	model					1238:1242	a PID rat model	1228:1242	a PID rat model	1228:1242	MATERIALS AND METHODS The anti-inflammatory effects of Penyanling were studied on a PID rat model and a lipopolysaccharides (LPS)-stimulated THP-1 cell line.
32979412	20	124	theme	phenolics	1725:1733	arg1	contents					1695:1702	The contents	1691:1702	The contents of total flavonoids, phenolics, and saponins	1691:1747	The contents of total flavonoids, phenolics, and saponins were quantified.
32979412	24	125	theme	molecular	2483:2491	arg1	weights					2493:2499	their accurate molecular weights	2468:2499	their accurate molecular weights	2468:2499	Seventy-six substances were identified by their accurate molecular weights, mass fragment patterns, retention times, and standards if available.
32979412	21	126	theme	chemotactic	2007:2017	arg1	MCP					2028:2030	monocyte chemotactic protein (MCP)-1	1998:2033	monocyte chemotactic protein (MCP)-1	1998:2033	RESULTS The anti-inflammatory effects of Penyanling were observed on PID rats, such as suppressing the infiltrations of lymphocytes and neutrophils in the uterine tube, decreasing the release of interleukin (IL)-1β, IL-6, IL-8, and monocyte chemotactic protein (MCP)-1, and promoting the production of lipoxin A4 (LXA4).
32979412	23	127	theme	inflammation	2412:2423	arg1	resolution					2398:2407	the resolution	2394:2407	the resolution of inflammation	2394:2423	Besides, it could promote the expression of formyl peptide receptor 2 (FPR2), which suggested its effect on enhancing the resolution of inflammation.
34380066	9	0	theme	flavonoid	1585:1593	arg1	compounds					1595:1603	phenolic and flavonoid compounds	1572:1603	compounds	1595:1603	RESULTS HME-PG showed high levels of phenolic and flavonoid compounds.
34380066	4	1	theme	STUDY	605:609	arg1	AIM					598:600	AIM	598:600	AIM OF STUDY The current study	598:627	AIM OF STUDY The current study was designed to evaluate the antioxidant and anti-inflammatory effects and toxicity of the hydromethanolic extract of the leaves from P. guineense (HME-PG), as well as to investigate the chemical composition.
34380066	15	2	theme	traditional	2576:2586	arg1	use					2588:2590	the safe traditional use	2567:2590	the safe traditional use of P. guineense	2567:2606	CONCLUSIONS The present study showed that HME-PG has antioxidant and anti-inflammatory properties, and no toxicity was detected after acute or subacute treatment with HME-PG, showing the possibility for the safe traditional use of P. guineense.
34380066	7	3	dep	pretreated	1122:1131	arg1	received					1184:1191	received	1184:1191	received carrageenan via paw injection (edema, cold sensitivity and mechanical hyperalgesia were analyzed) or pleural injection (leukocyte migration was analyzed after 4 h) and for nociception using the formalin model	1184:1400	Swiss mice were orally (p.o.) pretreated with HME-PG (30, 100 and 300 mg/kg), and after 1 h received carrageenan via paw injection (edema, cold sensitivity and mechanical hyperalgesia were analyzed) or pleural injection (leukocyte migration was analyzed after 4 h) and for nociception using the formalin model.
34380066	7	3	dep	pretreated	1122:1131	arg1	p.o.					1116:1119	p.o.	1116:1119	p.o.	1116:1119	Swiss mice were orally (p.o.) pretreated with HME-PG (30, 100 and 300 mg/kg), and after 1 h received carrageenan via paw injection (edema, cold sensitivity and mechanical hyperalgesia were analyzed) or pleural injection (leukocyte migration was analyzed after 4 h) and for nociception using the formalin model.
34380066	14	4	theme	Toxicity	2293:2300	arg1	tests					2302:2306	Toxicity tests	2293:2306	Toxicity tests	2293:2306	Toxicity tests did not cause signs of toxicity in the treated animals.
34380066	7	5	dep	HME-PG	1138:1143	arg1	300 mg/kg					1158:1166	300 mg/kg	1158:1166	300 mg/kg	1158:1166	Swiss mice were orally (p.o.) pretreated with HME-PG (30, 100 and 300 mg/kg), and after 1 h received carrageenan via paw injection (edema, cold sensitivity and mechanical hyperalgesia were analyzed) or pleural injection (leukocyte migration was analyzed after 4 h) and for nociception using the formalin model.
34380066	7	5	dep	HME-PG	1138:1143	arg1	100					1150:1152	100	1150:1152	100	1150:1152	Swiss mice were orally (p.o.) pretreated with HME-PG (30, 100 and 300 mg/kg), and after 1 h received carrageenan via paw injection (edema, cold sensitivity and mechanical hyperalgesia were analyzed) or pleural injection (leukocyte migration was analyzed after 4 h) and for nociception using the formalin model.
34380066	0	6	theme	Swartz	135:140	arg1	extract					106:112	the hydromethanolic extract	86:112	the hydromethanolic extract of Psidium guineense Swartz	86:140	Chemical analysis and antioxidant, anti-inflammatory and toxicological evaluations of the hydromethanolic extract of Psidium guineense Swartz leaves.
34380066	4	7	dep	STUDY	605:609	arg1	study					623:627	The current study	611:627	STUDY The current study	605:627	AIM OF STUDY The current study was designed to evaluate the antioxidant and anti-inflammatory effects and toxicity of the hydromethanolic extract of the leaves from P. guineense (HME-PG), as well as to investigate the chemical composition.
34380066	2	8	theme	research	313:320	arg1	group					322:326	Our research group	309:326	Our research group	309:326	Our research group studied an essential oil obtained from its leaves and reported anti-inflammatory and analgesic properties.
34380066	5	9	theme	MATERIALS	838:846	arg1	HME-PG					860:865	MATERIALS AND METHODS HME-PG	838:865	MATERIALS AND METHODS HME-PG	838:865	MATERIALS AND METHODS HME-PG was chemically investigated by Ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS).
34380066	4	10	theme	hydromethanolic	720:734	arg1	extract					736:742	the hydromethanolic extract	716:742	the hydromethanolic extract of the leaves from P. guineense (HME-PG)	716:783	AIM OF STUDY The current study was designed to evaluate the antioxidant and anti-inflammatory effects and toxicity of the hydromethanolic extract of the leaves from P. guineense (HME-PG), as well as to investigate the chemical composition.
34380066	11	11	theme	48.14 μg/mL	1828:1838	arg1	IC50					1820:1823	an IC50	1817:1823	an IC50 of 48.14 μg/mL in the MDA assay	1817:1855	HME-PG exhibited an IC50 of 48.14 μg/mL in the MDA assay and an IC50 of 45.15 μg/mL in the DPPH test.
34380066	11	11	theme	48.14 μg/mL	1828:1838	arg1	IC50					1864:1867	an IC50	1861:1867	an IC50 of 45.15 μg/mL	1861:1882	HME-PG exhibited an IC50 of 48.14 μg/mL in the MDA assay and an IC50 of 45.15 μg/mL in the DPPH test.
34380066	11	12	theme	DPPH	1891:1894	arg1	test					1896:1899	the DPPH test	1887:1899	the DPPH test	1887:1899	HME-PG exhibited an IC50 of 48.14 μg/mL in the MDA assay and an IC50 of 45.15 μg/mL in the DPPH test.
34380066	15	13	theme	antioxidant	2417:2427	arg1	properties					2451:2460	antioxidant and anti-inflammatory properties	2417:2460	antioxidant and anti-inflammatory properties	2417:2460	CONCLUSIONS The present study showed that HME-PG has antioxidant and anti-inflammatory properties, and no toxicity was detected after acute or subacute treatment with HME-PG, showing the possibility for the safe traditional use of P. guineense.
34380066	5	14	theme	METHODS	852:858	arg1	HME-PG					860:865	MATERIALS AND METHODS HME-PG	838:865	MATERIALS AND METHODS HME-PG	838:865	MATERIALS AND METHODS HME-PG was chemically investigated by Ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS).
34380066	4	15	theme	extract	736:742	arg1	toxicity					704:711	toxicity	704:711	toxicity	704:711	AIM OF STUDY The current study was designed to evaluate the antioxidant and anti-inflammatory effects and toxicity of the hydromethanolic extract of the leaves from P. guineense (HME-PG), as well as to investigate the chemical composition.
34380066	4	15	theme	extract	736:742	arg1	effects					692:698	antioxidant and anti-inflammatory effects	658:698	antioxidant and anti-inflammatory effects	658:698	AIM OF STUDY The current study was designed to evaluate the antioxidant and anti-inflammatory effects and toxicity of the hydromethanolic extract of the leaves from P. guineense (HME-PG), as well as to investigate the chemical composition.
34380066	12	16	from	points	1990:1995	arg1	response					2050:2057	the response	2046:2057	the response to cold 3 and 4 h	2046:2075	The treatment with HME-PG (100 and 300 mg/kg) significantly inhibited edema at all time points evaluated, mechanical hyperalgesia after 4 h and the response to cold 3 and 4 h after carrageenan injection and anti-nociceptive effects in both phases of formalin nociception.
34380066	12	16	from	points	1990:1995	arg1	edema					1972:1976	edema	1972:1976	edema at all time points evaluated	1972:2005	The treatment with HME-PG (100 and 300 mg/kg) significantly inhibited edema at all time points evaluated, mechanical hyperalgesia after 4 h and the response to cold 3 and 4 h after carrageenan injection and anti-nociceptive effects in both phases of formalin nociception.
34380066	12	16	from	points	1990:1995	arg1	hyperalgesia					2019:2030	mechanical hyperalgesia	2008:2030	mechanical hyperalgesia after 4 h	2008:2040	The treatment with HME-PG (100 and 300 mg/kg) significantly inhibited edema at all time points evaluated, mechanical hyperalgesia after 4 h and the response to cold 3 and 4 h after carrageenan injection and anti-nociceptive effects in both phases of formalin nociception.
34380066	5	17	theme	tandem	943:948	arg1	UHPLC-MS/MS					969:979	UHPLC-MS/MS	969:979	UHPLC-MS/MS	969:979	MATERIALS AND METHODS HME-PG was chemically investigated by Ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS).
34380066	5	17	theme	tandem	943:948	arg1	spectrometry					955:966	tandem mass spectrometry	943:966	Ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS)	898:980	MATERIALS AND METHODS HME-PG was chemically investigated by Ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS).
34380066	8	18	theme	subacute	1423:1430	arg1	toxicity					1442:1449	Acute (14 days) and subacute (28 days) toxicity	1403:1449	Acute (14 days) and subacute (28 days) toxicity	1403:1449	Acute (14 days) and subacute (28 days) toxicity was assessed with female Wistar rats orally treated with 500 and 2000 mg/kg HME-PG.
34380066	4	19	theme	the	747:749	arg1	extract					736:742	the hydromethanolic extract	716:742	the hydromethanolic extract of the leaves from P. guineense (HME-PG)	716:783	AIM OF STUDY The current study was designed to evaluate the antioxidant and anti-inflammatory effects and toxicity of the hydromethanolic extract of the leaves from P. guineense (HME-PG), as well as to investigate the chemical composition.
34380066	1	20	theme	ETHNOPHARMACOLOGICAL	150:169	arg1	guineense					189:197	ETHNOPHARMACOLOGICAL RELEVANCE Psidium guineense	150:197	ETHNOPHARMACOLOGICAL RELEVANCE Psidium guineense	150:197	ETHNOPHARMACOLOGICAL RELEVANCE Psidium guineense, popularly known as "araçá-do-campo", is used in popular medicine for the treatment of inflammatory diseases.
34380066	12	21	with	treatment	1906:1914	arg1	HME-PG					1921:1926	HME-PG	1921:1926	HME-PG	1921:1926	The treatment with HME-PG (100 and 300 mg/kg) significantly inhibited edema at all time points evaluated, mechanical hyperalgesia after 4 h and the response to cold 3 and 4 h after carrageenan injection and anti-nociceptive effects in both phases of formalin nociception.
34380066	15	22	theme	present	2380:2386	arg1	study					2388:2392	The present study	2376:2392	The present study	2376:2392	CONCLUSIONS The present study showed that HME-PG has antioxidant and anti-inflammatory properties, and no toxicity was detected after acute or subacute treatment with HME-PG, showing the possibility for the safe traditional use of P. guineense.
34380066	5	23	theme	Ultra-high	898:907	arg1	chromatography					928:941	Ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS)	898:980	Ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS)	898:980	MATERIALS AND METHODS HME-PG was chemically investigated by Ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS).
34380066	11	24	from	assay	1851:1855	arg1	IC50					1820:1823	an IC50	1817:1823	an IC50 of 48.14 μg/mL in the MDA assay	1817:1855	HME-PG exhibited an IC50 of 48.14 μg/mL in the MDA assay and an IC50 of 45.15 μg/mL in the DPPH test.
34380066	11	24	from	assay	1851:1855	arg1	IC50					1864:1867	an IC50	1861:1867	an IC50 of 45.15 μg/mL	1861:1882	HME-PG exhibited an IC50 of 48.14 μg/mL in the MDA assay and an IC50 of 45.15 μg/mL in the DPPH test.
34380066	5	25	theme	liquid	921:926	arg1	chromatography					928:941	Ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS)	898:980	Ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS)	898:980	MATERIALS AND METHODS HME-PG was chemically investigated by Ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS).
34380066	11	26	from	IC50	1820:1823	arg1	assay					1851:1855	the MDA assay	1843:1855	the MDA assay	1843:1855	HME-PG exhibited an IC50 of 48.14 μg/mL in the MDA assay and an IC50 of 45.15 μg/mL in the DPPH test.
34380066	0	27	theme	extract	106:112	arg1	evaluations					71:81	antioxidant, anti-inflammatory and toxicological evaluations	22:81	antioxidant, anti-inflammatory and toxicological evaluations of the hydromethanolic extract of Psidium guineense Swartz	22:140	Chemical analysis and antioxidant, anti-inflammatory and toxicological evaluations of the hydromethanolic extract of Psidium guineense Swartz leaves.
34380066	0	27	theme	extract	106:112	arg1	analysis					9:16	Chemical analysis	0:16	Chemical analysis	0:16	Chemical analysis and antioxidant, anti-inflammatory and toxicological evaluations of the hydromethanolic extract of Psidium guineense Swartz leaves.
34380066	13	28	theme	leukocyte	2225:2233	arg1	migration					2235:2243	leukocyte migration	2225:2243	leukocyte migration	2225:2243	All oral HME-PG treatments significantly inhibited leukocyte migration and plasma extravasation in the pleurisy model.
34380066	11	29	from	48.14 μg/mL	1828:1838	arg1	assay					1851:1855	the MDA assay	1843:1855	the MDA assay	1843:1855	HME-PG exhibited an IC50 of 48.14 μg/mL in the MDA assay and an IC50 of 45.15 μg/mL in the DPPH test.
34380066	5	30	dep	chromatography	928:941	arg1	UHPLC-MS/MS					969:979	UHPLC-MS/MS	969:979	UHPLC-MS/MS	969:979	MATERIALS AND METHODS HME-PG was chemically investigated by Ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS).
34380066	5	30	dep	chromatography	928:941	arg1	spectrometry					955:966	tandem mass spectrometry	943:966	Ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS)	898:980	MATERIALS AND METHODS HME-PG was chemically investigated by Ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS).
34380066	11	31	theme	MDA	1847:1849	arg1	assay					1851:1855	the MDA assay	1843:1855	the MDA assay	1843:1855	HME-PG exhibited an IC50 of 48.14 μg/mL in the MDA assay and an IC50 of 45.15 μg/mL in the DPPH test.
34380066	15	32	theme	P.	2595:2596	arg1	guineense					2598:2606	P. guineense	2595:2606	P. guineense	2595:2606	CONCLUSIONS The present study showed that HME-PG has antioxidant and anti-inflammatory properties, and no toxicity was detected after acute or subacute treatment with HME-PG, showing the possibility for the safe traditional use of P. guineense.
34380066	4	33	from	guineense	766:774	arg1	extract					736:742	the hydromethanolic extract	716:742	the hydromethanolic extract of the leaves from P. guineense (HME-PG)	716:783	AIM OF STUDY The current study was designed to evaluate the antioxidant and anti-inflammatory effects and toxicity of the hydromethanolic extract of the leaves from P. guineense (HME-PG), as well as to investigate the chemical composition.
34380066	7	34	theme	leukocyte	1313:1321	arg1	migration					1323:1331	leukocyte migration	1313:1331	leukocyte migration	1313:1331	Swiss mice were orally (p.o.) pretreated with HME-PG (30, 100 and 300 mg/kg), and after 1 h received carrageenan via paw injection (edema, cold sensitivity and mechanical hyperalgesia were analyzed) or pleural injection (leukocyte migration was analyzed after 4 h) and for nociception using the formalin model.
34380066	8	35	theme	Wistar	1476:1481	arg1	rats					1483:1486	female Wistar rats	1469:1486	female Wistar rats orally treated with 500 and 2000 mg/kg HME-PG	1469:1532	Acute (14 days) and subacute (28 days) toxicity was assessed with female Wistar rats orally treated with 500 and 2000 mg/kg HME-PG.
34380066	0	36	theme	Chemical	0:7	arg1	analysis					9:16	Chemical analysis	0:16	Chemical analysis	0:16	Chemical analysis and antioxidant, anti-inflammatory and toxicological evaluations of the hydromethanolic extract of Psidium guineense Swartz leaves.
34380066	1	37	theme	inflammatory	286:297	arg1	diseases					299:306	inflammatory diseases	286:306	inflammatory diseases	286:306	ETHNOPHARMACOLOGICAL RELEVANCE Psidium guineense, popularly known as "araçá-do-campo", is used in popular medicine for the treatment of inflammatory diseases.
34380066	15	38	theme	anti-inflammatory	2433:2449	arg1	properties					2451:2460	antioxidant and anti-inflammatory properties	2417:2460	antioxidant and anti-inflammatory properties	2417:2460	CONCLUSIONS The present study showed that HME-PG has antioxidant and anti-inflammatory properties, and no toxicity was detected after acute or subacute treatment with HME-PG, showing the possibility for the safe traditional use of P. guineense.
34380066	12	39	dep	HME-PG	1921:1926	arg1	300 mg/kg					1937:1945	300 mg/kg	1937:1945	300 mg/kg	1937:1945	The treatment with HME-PG (100 and 300 mg/kg) significantly inhibited edema at all time points evaluated, mechanical hyperalgesia after 4 h and the response to cold 3 and 4 h after carrageenan injection and anti-nociceptive effects in both phases of formalin nociception.
34380066	12	39	dep	HME-PG	1921:1926	arg1	100					1929:1931	100	1929:1931	100	1929:1931	The treatment with HME-PG (100 and 300 mg/kg) significantly inhibited edema at all time points evaluated, mechanical hyperalgesia after 4 h and the response to cold 3 and 4 h after carrageenan injection and anti-nociceptive effects in both phases of formalin nociception.
34380066	12	40	theme	carrageenan	2083:2093	arg1	injection					2095:2103	carrageenan injection	2083:2103	carrageenan injection	2083:2103	The treatment with HME-PG (100 and 300 mg/kg) significantly inhibited edema at all time points evaluated, mechanical hyperalgesia after 4 h and the response to cold 3 and 4 h after carrageenan injection and anti-nociceptive effects in both phases of formalin nociception.
34380066	7	41	dep	injection	1302:1310	arg1	analyzed					1337:1344	analyzed	1337:1344	was analyzed after 4 h	1333:1354	Swiss mice were orally (p.o.) pretreated with HME-PG (30, 100 and 300 mg/kg), and after 1 h received carrageenan via paw injection (edema, cold sensitivity and mechanical hyperalgesia were analyzed) or pleural injection (leukocyte migration was analyzed after 4 h) and for nociception using the formalin model.
34380066	9	42	theme	high	1557:1560	arg1	levels					1562:1567	high levels	1557:1567	high levels of phenolic and flavonoid compounds	1557:1603	RESULTS HME-PG showed high levels of phenolic and flavonoid compounds.
34380066	10	43	theme	UHPLC-MS/MS	1645:1655	arg1	analysis					1657:1664	UHPLC-MS/MS analysis	1645:1664	UHPLC-MS/MS analysis	1645:1664	Six compounds were identified based on UHPLC-MS/MS analysis, including gallic acid, quercetin, 3'-formyl-2',4',6'-trihydroxy-5'-methyldihydrochalcone, vanillic acid, ursolic acid and corilagin.
34380066	7	44	dep	analyzed	1281:1288	arg1	edema					1224:1228	edema	1224:1228	edema	1224:1228	Swiss mice were orally (p.o.) pretreated with HME-PG (30, 100 and 300 mg/kg), and after 1 h received carrageenan via paw injection (edema, cold sensitivity and mechanical hyperalgesia were analyzed) or pleural injection (leukocyte migration was analyzed after 4 h) and for nociception using the formalin model.
34380066	1	45	theme	Psidium	181:187	arg1	guineense					189:197	ETHNOPHARMACOLOGICAL RELEVANCE Psidium guineense	150:197	ETHNOPHARMACOLOGICAL RELEVANCE Psidium guineense	150:197	ETHNOPHARMACOLOGICAL RELEVANCE Psidium guineense, popularly known as "araçá-do-campo", is used in popular medicine for the treatment of inflammatory diseases.
34380066	2	46	theme	reported	382:389	arg1	properties					423:432	reported anti-inflammatory and analgesic properties	382:432	reported anti-inflammatory and analgesic properties	382:432	Our research group studied an essential oil obtained from its leaves and reported anti-inflammatory and analgesic properties.
34380066	1	47	theme	popular	248:254	arg1	medicine					256:263	popular medicine	248:263	popular medicine	248:263	ETHNOPHARMACOLOGICAL RELEVANCE Psidium guineense, popularly known as "araçá-do-campo", is used in popular medicine for the treatment of inflammatory diseases.
34380066	7	48	theme	cold	1231:1234	arg1	sensitivity					1236:1246	cold sensitivity	1231:1246	cold sensitivity	1231:1246	Swiss mice were orally (p.o.) pretreated with HME-PG (30, 100 and 300 mg/kg), and after 1 h received carrageenan via paw injection (edema, cold sensitivity and mechanical hyperalgesia were analyzed) or pleural injection (leukocyte migration was analyzed after 4 h) and for nociception using the formalin model.
34380066	14	49	theme	toxicity	2331:2338	arg1	signs					2322:2326	signs	2322:2326	signs of toxicity	2322:2338	Toxicity tests did not cause signs of toxicity in the treated animals.
34380066	10	50	theme	gallic	1677:1682	arg1	acid					1684:1687	gallic acid	1677:1687	gallic acid	1677:1687	Six compounds were identified based on UHPLC-MS/MS analysis, including gallic acid, quercetin, 3'-formyl-2',4',6'-trihydroxy-5'-methyldihydrochalcone, vanillic acid, ursolic acid and corilagin.
34380066	15	51	theme	subacute	2507:2514	arg1	treatment					2516:2524	acute or subacute treatment	2498:2524	acute or subacute treatment with HME-PG	2498:2536	CONCLUSIONS The present study showed that HME-PG has antioxidant and anti-inflammatory properties, and no toxicity was detected after acute or subacute treatment with HME-PG, showing the possibility for the safe traditional use of P. guineense.
34380066	9	52	theme	compounds	1595:1603	arg1	levels					1562:1567	high levels	1557:1567	high levels of phenolic and flavonoid compounds	1557:1603	RESULTS HME-PG showed high levels of phenolic and flavonoid compounds.
34380066	15	53	contain	has	2413:2415	arg2	properties					2451:2460	antioxidant and anti-inflammatory properties	2417:2460	antioxidant and anti-inflammatory properties	2417:2460	CONCLUSIONS The present study showed that HME-PG has antioxidant and anti-inflammatory properties, and no toxicity was detected after acute or subacute treatment with HME-PG, showing the possibility for the safe traditional use of P. guineense.
34380066	15	53	contain	has	2413:2415	arg1	HME-PG					2406:2411	HME-PG	2406:2411	HME-PG	2406:2411	CONCLUSIONS The present study showed that HME-PG has antioxidant and anti-inflammatory properties, and no toxicity was detected after acute or subacute treatment with HME-PG, showing the possibility for the safe traditional use of P. guineense.
34380066	15	54	theme	acute	2498:2502	arg1	treatment					2516:2524	acute or subacute treatment	2498:2524	acute or subacute treatment with HME-PG	2498:2536	CONCLUSIONS The present study showed that HME-PG has antioxidant and anti-inflammatory properties, and no toxicity was detected after acute or subacute treatment with HME-PG, showing the possibility for the safe traditional use of P. guineense.
34380066	15	55	with	treatment	2516:2524	arg1	HME-PG					2531:2536	HME-PG	2531:2536	HME-PG	2531:2536	CONCLUSIONS The present study showed that HME-PG has antioxidant and anti-inflammatory properties, and no toxicity was detected after acute or subacute treatment with HME-PG, showing the possibility for the safe traditional use of P. guineense.
34380066	13	56	theme	oral	2178:2181	arg1	treatments					2190:2199	All oral HME-PG treatments	2174:2199	All oral HME-PG treatments	2174:2199	All oral HME-PG treatments significantly inhibited leukocyte migration and plasma extravasation in the pleurisy model.
34380066	3	57	theme	folk	552:555	arg1	use					557:559	the traditional folk use	536:559	the traditional folk use of this plant	536:573	However, to date, the anti-inflammatory actions of the leaf extract have not been evaluated although the traditional folk use of this plant has these indications.
34380066	4	58	theme	antioxidant	658:668	arg1	effects					692:698	antioxidant and anti-inflammatory effects	658:698	antioxidant and anti-inflammatory effects	658:698	AIM OF STUDY The current study was designed to evaluate the antioxidant and anti-inflammatory effects and toxicity of the hydromethanolic extract of the leaves from P. guineense (HME-PG), as well as to investigate the chemical composition.
34380066	15	59	theme	safe	2571:2574	arg1	use					2588:2590	the safe traditional use	2567:2590	the safe traditional use of P. guineense	2567:2606	CONCLUSIONS The present study showed that HME-PG has antioxidant and anti-inflammatory properties, and no toxicity was detected after acute or subacute treatment with HME-PG, showing the possibility for the safe traditional use of P. guineense.
34380066	13	60	theme	pleurisy	2277:2284	arg1	model					2286:2290	the pleurisy model	2273:2290	the pleurisy model	2273:2290	All oral HME-PG treatments significantly inhibited leukocyte migration and plasma extravasation in the pleurisy model.
34380066	7	61	theme	formalin	1387:1394	arg1	model					1396:1400	the formalin model	1383:1400	the formalin model	1383:1400	Swiss mice were orally (p.o.) pretreated with HME-PG (30, 100 and 300 mg/kg), and after 1 h received carrageenan via paw injection (edema, cold sensitivity and mechanical hyperalgesia were analyzed) or pleural injection (leukocyte migration was analyzed after 4 h) and for nociception using the formalin model.
34380066	4	62	theme	anti-inflammatory	674:690	arg1	effects					692:698	antioxidant and anti-inflammatory effects	658:698	antioxidant and anti-inflammatory effects	658:698	AIM OF STUDY The current study was designed to evaluate the antioxidant and anti-inflammatory effects and toxicity of the hydromethanolic extract of the leaves from P. guineense (HME-PG), as well as to investigate the chemical composition.
34380066	7	63	theme	Swiss	1092:1096	arg1	mice					1098:1101	Swiss mice	1092:1101	Swiss mice	1092:1101	Swiss mice were orally (p.o.) pretreated with HME-PG (30, 100 and 300 mg/kg), and after 1 h received carrageenan via paw injection (edema, cold sensitivity and mechanical hyperalgesia were analyzed) or pleural injection (leukocyte migration was analyzed after 4 h) and for nociception using the formalin model.
34380066	3	64	theme	plant	569:573	arg1	use					557:559	the traditional folk use	536:559	the traditional folk use of this plant	536:573	However, to date, the anti-inflammatory actions of the leaf extract have not been evaluated although the traditional folk use of this plant has these indications.
34380066	1	65	theme	araçá-do-campo	220:233	arg1	"					234:234	"araçá-do-campo"	219:234	"araçá-do-campo"	219:234	ETHNOPHARMACOLOGICAL RELEVANCE Psidium guineense, popularly known as "araçá-do-campo", is used in popular medicine for the treatment of inflammatory diseases.
34380066	6	66	theme	antioxidant	987:997	arg1	activity					999:1006	The antioxidant activity	983:1006	The antioxidant activity	983:1006	The antioxidant activity was evaluated with 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and malondialdehyde (MDA).
34380066	13	67	theme	HME-PG	2183:2188	arg1	treatments					2190:2199	All oral HME-PG treatments	2174:2199	All oral HME-PG treatments	2174:2199	All oral HME-PG treatments significantly inhibited leukocyte migration and plasma extravasation in the pleurisy model.
34380066	12	68	theme	mechanical	2008:2017	arg1	hyperalgesia					2019:2030	mechanical hyperalgesia	2008:2030	mechanical hyperalgesia after 4 h	2008:2040	The treatment with HME-PG (100 and 300 mg/kg) significantly inhibited edema at all time points evaluated, mechanical hyperalgesia after 4 h and the response to cold 3 and 4 h after carrageenan injection and anti-nociceptive effects in both phases of formalin nociception.
34380066	13	69	theme	plasma	2249:2254	arg1	extravasation					2256:2268	plasma extravasation	2249:2268	plasma extravasation	2249:2268	All oral HME-PG treatments significantly inhibited leukocyte migration and plasma extravasation in the pleurisy model.
34380066	11	70	theme	45.15 μg/mL	1872:1882	arg1	IC50					1820:1823	an IC50	1817:1823	an IC50 of 48.14 μg/mL in the MDA assay	1817:1855	HME-PG exhibited an IC50 of 48.14 μg/mL in the MDA assay and an IC50 of 45.15 μg/mL in the DPPH test.
34380066	11	70	theme	45.15 μg/mL	1872:1882	arg1	IC50					1864:1867	an IC50	1861:1867	an IC50 of 45.15 μg/mL	1861:1882	HME-PG exhibited an IC50 of 48.14 μg/mL in the MDA assay and an IC50 of 45.15 μg/mL in the DPPH test.
34380066	11	71	from	IC50	1864:1867	arg1	assay					1851:1855	the MDA assay	1843:1855	the MDA assay	1843:1855	HME-PG exhibited an IC50 of 48.14 μg/mL in the MDA assay and an IC50 of 45.15 μg/mL in the DPPH test.
34380066	12	72	theme	time	1985:1988	arg1	points					1990:1995	all time points	1981:1995	all time points evaluated	1981:2005	The treatment with HME-PG (100 and 300 mg/kg) significantly inhibited edema at all time points evaluated, mechanical hyperalgesia after 4 h and the response to cold 3 and 4 h after carrageenan injection and anti-nociceptive effects in both phases of formalin nociception.
34380066	5	73	theme	mass	950:953	arg1	UHPLC-MS/MS					969:979	UHPLC-MS/MS	969:979	UHPLC-MS/MS	969:979	MATERIALS AND METHODS HME-PG was chemically investigated by Ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS).
34380066	5	73	theme	mass	950:953	arg1	spectrometry					955:966	tandem mass spectrometry	943:966	Ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS)	898:980	MATERIALS AND METHODS HME-PG was chemically investigated by Ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS).
34380066	3	74	theme	extract	495:501	arg1	actions					475:481	the anti-inflammatory actions	453:481	the anti-inflammatory actions of the leaf extract	453:501	However, to date, the anti-inflammatory actions of the leaf extract have not been evaluated although the traditional folk use of this plant has these indications.
34380066	7	75	theme	paw	1209:1211	arg1	injection					1213:1221	paw injection	1209:1221	paw injection (edema, cold sensitivity and mechanical hyperalgesia were analyzed)	1209:1289	Swiss mice were orally (p.o.) pretreated with HME-PG (30, 100 and 300 mg/kg), and after 1 h received carrageenan via paw injection (edema, cold sensitivity and mechanical hyperalgesia were analyzed) or pleural injection (leukocyte migration was analyzed after 4 h) and for nociception using the formalin model.
34380066	10	76	theme	ursolic	1772:1778	arg1	acid					1780:1783	ursolic acid	1772:1783	ursolic acid	1772:1783	Six compounds were identified based on UHPLC-MS/MS analysis, including gallic acid, quercetin, 3'-formyl-2',4',6'-trihydroxy-5'-methyldihydrochalcone, vanillic acid, ursolic acid and corilagin.
34380066	1	77	theme	RELEVANCE	171:179	arg1	guineense					189:197	ETHNOPHARMACOLOGICAL RELEVANCE Psidium guineense	150:197	ETHNOPHARMACOLOGICAL RELEVANCE Psidium guineense	150:197	ETHNOPHARMACOLOGICAL RELEVANCE Psidium guineense, popularly known as "araçá-do-campo", is used in popular medicine for the treatment of inflammatory diseases.
34380066	0	78	theme	hydromethanolic	90:104	arg1	extract					106:112	the hydromethanolic extract	86:112	the hydromethanolic extract of Psidium guineense Swartz	86:140	Chemical analysis and antioxidant, anti-inflammatory and toxicological evaluations of the hydromethanolic extract of Psidium guineense Swartz leaves.
34380066	15	79	dep	CONCLUSIONS	2364:2374	arg1	showed					2394:2399	showed	2394:2399	showed that HME-PG has antioxidant and anti-inflammatory properties, and no toxicity was detected after acute or subacute treatment with HME-PG, showing the possibility for the safe traditional use of P. guineense	2394:2606	CONCLUSIONS The present study showed that HME-PG has antioxidant and anti-inflammatory properties, and no toxicity was detected after acute or subacute treatment with HME-PG, showing the possibility for the safe traditional use of P. guineense.
34380066	5	80	theme	performance	909:919	arg1	chromatography					928:941	Ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS)	898:980	Ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS)	898:980	MATERIALS AND METHODS HME-PG was chemically investigated by Ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS).
34380066	15	81	theme	guineense	2598:2606	arg1	use					2588:2590	the safe traditional use	2567:2590	the safe traditional use of P. guineense	2567:2606	CONCLUSIONS The present study showed that HME-PG has antioxidant and anti-inflammatory properties, and no toxicity was detected after acute or subacute treatment with HME-PG, showing the possibility for the safe traditional use of P. guineense.
34380066	8	82	theme	female	1469:1474	arg1	rats					1483:1486	female Wistar rats	1469:1486	female Wistar rats orally treated with 500 and 2000 mg/kg HME-PG	1469:1532	Acute (14 days) and subacute (28 days) toxicity was assessed with female Wistar rats orally treated with 500 and 2000 mg/kg HME-PG.
34380066	2	83	dep	its	367:369	arg1	leaves					371:376	leaves	371:376	leaves	371:376	Our research group studied an essential oil obtained from its leaves and reported anti-inflammatory and analgesic properties.
34380066	12	84	theme	formalin	2152:2159	arg1	nociception					2161:2171	formalin nociception	2152:2171	formalin nociception	2152:2171	The treatment with HME-PG (100 and 300 mg/kg) significantly inhibited edema at all time points evaluated, mechanical hyperalgesia after 4 h and the response to cold 3 and 4 h after carrageenan injection and anti-nociceptive effects in both phases of formalin nociception.
34380066	2	85	theme	analgesic	413:421	arg1	properties					423:432	reported anti-inflammatory and analgesic properties	382:432	reported anti-inflammatory and analgesic properties	382:432	Our research group studied an essential oil obtained from its leaves and reported anti-inflammatory and analgesic properties.
34380066	4	86	dep	the	747:749	arg1	leaves					751:756	leaves	751:756	leaves	751:756	AIM OF STUDY The current study was designed to evaluate the antioxidant and anti-inflammatory effects and toxicity of the hydromethanolic extract of the leaves from P. guineense (HME-PG), as well as to investigate the chemical composition.
34380066	7	87	theme	pleural	1294:1300	arg1	injection					1302:1310	pleural injection	1294:1310	pleural injection (leukocyte migration was analyzed after 4 h)	1294:1355	Swiss mice were orally (p.o.) pretreated with HME-PG (30, 100 and 300 mg/kg), and after 1 h received carrageenan via paw injection (edema, cold sensitivity and mechanical hyperalgesia were analyzed) or pleural injection (leukocyte migration was analyzed after 4 h) and for nociception using the formalin model.
34380066	3	88	contain	has	575:577	arg2	indications					585:595	these indications	579:595	these indications	579:595	However, to date, the anti-inflammatory actions of the leaf extract have not been evaluated although the traditional folk use of this plant has these indications.
34380066	3	88	contain	has	575:577	arg1	use					557:559	the traditional folk use	536:559	the traditional folk use of this plant	536:573	However, to date, the anti-inflammatory actions of the leaf extract have not been evaluated although the traditional folk use of this plant has these indications.
34380066	12	89	theme	anti-nociceptive	2109:2124	arg1	effects					2126:2132	anti-nociceptive effects	2109:2132	anti-nociceptive effects	2109:2132	The treatment with HME-PG (100 and 300 mg/kg) significantly inhibited edema at all time points evaluated, mechanical hyperalgesia after 4 h and the response to cold 3 and 4 h after carrageenan injection and anti-nociceptive effects in both phases of formalin nociception.
34380066	2	90	theme	anti-inflammatory	391:407	arg1	properties					423:432	reported anti-inflammatory and analgesic properties	382:432	reported anti-inflammatory and analgesic properties	382:432	Our research group studied an essential oil obtained from its leaves and reported anti-inflammatory and analgesic properties.
34380066	0	91	theme	antioxidant	22:32	arg1	evaluations					71:81	antioxidant, anti-inflammatory and toxicological evaluations	22:81	antioxidant, anti-inflammatory and toxicological evaluations of the hydromethanolic extract of Psidium guineense Swartz	22:140	Chemical analysis and antioxidant, anti-inflammatory and toxicological evaluations of the hydromethanolic extract of Psidium guineense Swartz leaves.
34380066	1	92	theme	diseases	299:306	arg1	treatment					273:281	the treatment	269:281	the treatment of inflammatory diseases	269:306	ETHNOPHARMACOLOGICAL RELEVANCE Psidium guineense, popularly known as "araçá-do-campo", is used in popular medicine for the treatment of inflammatory diseases.
34380066	12	93	theme	nociception	2161:2171	arg1	phases					2142:2147	both phases	2137:2147	both phases of formalin nociception	2137:2171	The treatment with HME-PG (100 and 300 mg/kg) significantly inhibited edema at all time points evaluated, mechanical hyperalgesia after 4 h and the response to cold 3 and 4 h after carrageenan injection and anti-nociceptive effects in both phases of formalin nociception.
34380066	3	94	theme	leaf	490:493	arg1	extract					495:501	the leaf extract	486:501	the leaf extract	486:501	However, to date, the anti-inflammatory actions of the leaf extract have not been evaluated although the traditional folk use of this plant has these indications.
34380066	0	95	theme	anti-inflammatory	35:51	arg1	evaluations					71:81	antioxidant, anti-inflammatory and toxicological evaluations	22:81	antioxidant, anti-inflammatory and toxicological evaluations of the hydromethanolic extract of Psidium guineense Swartz	22:140	Chemical analysis and antioxidant, anti-inflammatory and toxicological evaluations of the hydromethanolic extract of Psidium guineense Swartz leaves.
34380066	8	96	dep	subacute	1423:1430	arg1	days					1436:1439	28 days	1433:1439	28 days	1433:1439	Acute (14 days) and subacute (28 days) toxicity was assessed with female Wistar rats orally treated with 500 and 2000 mg/kg HME-PG.
34380066	7	97	dep	injection	1213:1221	arg1	analyzed					1281:1288	analyzed	1281:1288	were analyzed	1276:1288	Swiss mice were orally (p.o.) pretreated with HME-PG (30, 100 and 300 mg/kg), and after 1 h received carrageenan via paw injection (edema, cold sensitivity and mechanical hyperalgesia were analyzed) or pleural injection (leukocyte migration was analyzed after 4 h) and for nociception using the formalin model.
34380066	0	98	theme	toxicological	57:69	arg1	evaluations					71:81	antioxidant, anti-inflammatory and toxicological evaluations	22:81	antioxidant, anti-inflammatory and toxicological evaluations of the hydromethanolic extract of Psidium guineense Swartz	22:140	Chemical analysis and antioxidant, anti-inflammatory and toxicological evaluations of the hydromethanolic extract of Psidium guineense Swartz leaves.
34380066	7	99	theme	mechanical	1252:1261	arg1	hyperalgesia					1263:1274	mechanical hyperalgesia	1252:1274	mechanical hyperalgesia	1252:1274	Swiss mice were orally (p.o.) pretreated with HME-PG (30, 100 and 300 mg/kg), and after 1 h received carrageenan via paw injection (edema, cold sensitivity and mechanical hyperalgesia were analyzed) or pleural injection (leukocyte migration was analyzed after 4 h) and for nociception using the formalin model.
34380066	4	100	theme	chemical	816:823	arg1	composition					825:835	the chemical composition	812:835	the chemical composition	812:835	AIM OF STUDY The current study was designed to evaluate the antioxidant and anti-inflammatory effects and toxicity of the hydromethanolic extract of the leaves from P. guineense (HME-PG), as well as to investigate the chemical composition.
34380066	4	101	dep	effects	692:698	arg1	the					654:656	the	654:656	the	654:656	AIM OF STUDY The current study was designed to evaluate the antioxidant and anti-inflammatory effects and toxicity of the hydromethanolic extract of the leaves from P. guineense (HME-PG), as well as to investigate the chemical composition.
34380066	10	102	theme	vanillic	1757:1764	arg1	acid					1766:1769	vanillic acid	1757:1769	vanillic acid	1757:1769	Six compounds were identified based on UHPLC-MS/MS analysis, including gallic acid, quercetin, 3'-formyl-2',4',6'-trihydroxy-5'-methyldihydrochalcone, vanillic acid, ursolic acid and corilagin.
34380066	4	103	theme	current	615:621	arg1	study					623:627	The current study	611:627	STUDY The current study	605:627	AIM OF STUDY The current study was designed to evaluate the antioxidant and anti-inflammatory effects and toxicity of the hydromethanolic extract of the leaves from P. guineense (HME-PG), as well as to investigate the chemical composition.
34380066	14	104	theme	treated	2347:2353	arg1	animals					2355:2361	the treated animals	2343:2361	the treated animals	2343:2361	Toxicity tests did not cause signs of toxicity in the treated animals.
34380066	2	105	theme	essential	339:347	arg1	oil					349:351	an essential oil	336:351	an essential oil obtained from its leaves and reported anti-inflammatory and analgesic properties	336:432	Our research group studied an essential oil obtained from its leaves and reported anti-inflammatory and analgesic properties.
34380066	4	106	from	extract	736:742	arg1	HME-PG					777:782	HME-PG	777:782	HME-PG	777:782	AIM OF STUDY The current study was designed to evaluate the antioxidant and anti-inflammatory effects and toxicity of the hydromethanolic extract of the leaves from P. guineense (HME-PG), as well as to investigate the chemical composition.
34380066	4	106	from	extract	736:742	arg1	guineense					766:774	P. guineense	763:774	P. guineense (HME-PG)	763:783	AIM OF STUDY The current study was designed to evaluate the antioxidant and anti-inflammatory effects and toxicity of the hydromethanolic extract of the leaves from P. guineense (HME-PG), as well as to investigate the chemical composition.
34380066	3	107	theme	anti-inflammatory	457:473	arg1	actions					475:481	the anti-inflammatory actions	453:481	the anti-inflammatory actions of the leaf extract	453:501	However, to date, the anti-inflammatory actions of the leaf extract have not been evaluated although the traditional folk use of this plant has these indications.
34380066	1	108	used	used	240:243	arg2	guineense					189:197	ETHNOPHARMACOLOGICAL RELEVANCE Psidium guineense	150:197	ETHNOPHARMACOLOGICAL RELEVANCE Psidium guineense	150:197	ETHNOPHARMACOLOGICAL RELEVANCE Psidium guineense, popularly known as "araçá-do-campo", is used in popular medicine for the treatment of inflammatory diseases.
34380066	9	109	theme	phenolic	1572:1579	arg1	compounds					1595:1603	phenolic and flavonoid compounds	1572:1603	compounds	1595:1603	RESULTS HME-PG showed high levels of phenolic and flavonoid compounds.
34380066	8	110	dep	Acute	1403:1407	arg1	days					1413:1416	14 days	1410:1416	14 days	1410:1416	Acute (14 days) and subacute (28 days) toxicity was assessed with female Wistar rats orally treated with 500 and 2000 mg/kg HME-PG.
34380066	8	111	theme	Acute	1403:1407	arg1	toxicity					1442:1449	Acute (14 days) and subacute (28 days) toxicity	1403:1449	Acute (14 days) and subacute (28 days) toxicity	1403:1449	Acute (14 days) and subacute (28 days) toxicity was assessed with female Wistar rats orally treated with 500 and 2000 mg/kg HME-PG.
34380066	3	112	theme	traditional	540:550	arg1	use					557:559	the traditional folk use	536:559	the traditional folk use of this plant	536:573	However, to date, the anti-inflammatory actions of the leaf extract have not been evaluated although the traditional folk use of this plant has these indications.
34784410	0	0	theme	low-grade	80:88	arg1	inflammation					99:110	obesity-related low-grade systemic inflammation	64:110	obesity-related low-grade systemic inflammation	64:110	Type 3 resistant starch from Canna edulis modulates obesity and obesity-related low-grade systemic inflammation in mice by regulating gut microbiota composition and metabolism.
34784410	5	1	theme	gene	756:759	arg1	sequencing					761:770	16S rRNA gene sequencing	747:770	16S rRNA gene sequencing	747:770	In the present study, obese mice were treated with Ce-RS3, and 16S rRNA gene sequencing and metabolomics were used to measure changes in gut microbiota and fecal metabolic profiles, respectively.
34784410	10	2	theme	fecal	1526:1530	arg1	transplantation					1532:1546	fecal transplantation	1526:1546	fecal transplantation	1526:1546	More importantly, we found that the anti-obesity effect of Ce-RS3 can be transferred by fecal transplantation.
34784410	11	3	theme	gut	1600:1602	arg1	changes					1615:1621	gut microbiota changes	1600:1621	gut microbiota changes	1600:1621	The beneficial effects of Ce-RS3 might derive from gut microbiota changes, which might improve obesity and metabolic inflammation by altering host-microbiota interactions with impacts on the metabolome.
34784410	2	4	dep	help	302:305	arg1	reshape					307:313	reshape	307:313	reshape	307:313	Several studies showed that appropriate modulation of gut microbiota could help reshape the metabolic profile of obese individuals, thereby altering the development of obesity.
34784410	5	5	from	changes	810:816	arg1	profiles					856:863	fecal metabolic profiles	840:863	fecal metabolic profiles	840:863	In the present study, obese mice were treated with Ce-RS3, and 16S rRNA gene sequencing and metabolomics were used to measure changes in gut microbiota and fecal metabolic profiles, respectively.
34784410	5	5	from	changes	810:816	arg1	microbiota					825:834	gut microbiota	821:834	gut microbiota	821:834	In the present study, obese mice were treated with Ce-RS3, and 16S rRNA gene sequencing and metabolomics were used to measure changes in gut microbiota and fecal metabolic profiles, respectively.
34784410	4	6	theme	microbial	587:595	arg1	community					597:605	the intestinal microbial community	572:605	the intestinal microbial community	572:605	Type 3 Resistant Starch from Canna edulis (Ce-RS3) is a dietary fiber that exerts potential effects on the intestinal microbial community; however, the metabolic landscape and anti-obesity mechanism remain unclear.
34784410	0	7	theme	obesity-related	64:78	arg1	inflammation					99:110	obesity-related low-grade systemic inflammation	64:110	obesity-related low-grade systemic inflammation	64:110	Type 3 resistant starch from Canna edulis modulates obesity and obesity-related low-grade systemic inflammation in mice by regulating gut microbiota composition and metabolism.
34784410	4	8	theme	Resistant	476:484	arg1	fiber					533:537	a dietary fiber	523:537	a dietary fiber that exerts potential effects on the intestinal microbial community	523:605	Type 3 Resistant Starch from Canna edulis (Ce-RS3) is a dietary fiber that exerts potential effects on the intestinal microbial community; however, the metabolic landscape and anti-obesity mechanism remain unclear.
34784410	4	8	theme	Resistant	476:484	arg1	Starch					486:491	Type 3 Resistant Starch	469:491	Type 3 Resistant Starch from Canna edulis (Ce-RS3)	469:518	Type 3 Resistant Starch from Canna edulis (Ce-RS3) is a dietary fiber that exerts potential effects on the intestinal microbial community; however, the metabolic landscape and anti-obesity mechanism remain unclear.
34784410	12	9	used	used	1781:1784	arg2	Ce-RS3					1767:1772	Ce-RS3	1767:1772	Ce-RS3	1767:1772	In conclusion, Ce-RS3 can be used as a prebiotic with potential value for the treatment of obesity.
34784410	12	9	used	used	1781:1784	arg2	prebiotic					1791:1799	a prebiotic	1789:1799	a prebiotic with potential value for the treatment of obesity	1789:1849	In conclusion, Ce-RS3 can be used as a prebiotic with potential value for the treatment of obesity.
34784410	4	10	theme	intestinal	576:585	arg1	community					597:605	the intestinal microbial community	572:605	the intestinal microbial community	572:605	Type 3 Resistant Starch from Canna edulis (Ce-RS3) is a dietary fiber that exerts potential effects on the intestinal microbial community; however, the metabolic landscape and anti-obesity mechanism remain unclear.
34784410	11	11	from	impacts	1725:1731	arg1	metabolome					1740:1749	the metabolome	1736:1749	the metabolome	1736:1749	The beneficial effects of Ce-RS3 might derive from gut microbiota changes, which might improve obesity and metabolic inflammation by altering host-microbiota interactions with impacts on the metabolome.
34784410	0	12	theme	systemic	90:97	arg1	inflammation					99:110	obesity-related low-grade systemic inflammation	64:110	obesity-related low-grade systemic inflammation	64:110	Type 3 resistant starch from Canna edulis modulates obesity and obesity-related low-grade systemic inflammation in mice by regulating gut microbiota composition and metabolism.
34784410	4	13	theme	Canna	498:502	arg1	edulis					504:509	Canna edulis	498:509	Canna edulis (Ce-RS3)	498:518	Type 3 Resistant Starch from Canna edulis (Ce-RS3) is a dietary fiber that exerts potential effects on the intestinal microbial community; however, the metabolic landscape and anti-obesity mechanism remain unclear.
34784410	4	13	theme	Canna	498:502	arg1	Ce-RS3					512:517	Ce-RS3	512:517	Ce-RS3	512:517	Type 3 Resistant Starch from Canna edulis (Ce-RS3) is a dietary fiber that exerts potential effects on the intestinal microbial community; however, the metabolic landscape and anti-obesity mechanism remain unclear.
34784410	0	14	from	edulis	35:40	arg1	starch					17:22	Type 3 resistant starch	0:22	Type 3 resistant starch from Canna edulis	0:40	Type 3 resistant starch from Canna edulis modulates obesity and obesity-related low-grade systemic inflammation in mice by regulating gut microbiota composition and metabolism.
34784410	7	15	theme	gut	1059:1061	arg1	structure					1074:1082	a healthy gut microbiota structure	1049:1082	a healthy gut microbiota structure	1049:1082	Ce-RS3 constructs a healthy gut microbiota structure and can enhance intestinal immunity and reduce metabolic inflammation.
34784410	4	16	theme	landscape	631:639	arg1	mechanism					658:666	the metabolic landscape and anti-obesity mechanism	617:666	the metabolic landscape and anti-obesity mechanism	617:666	Type 3 Resistant Starch from Canna edulis (Ce-RS3) is a dietary fiber that exerts potential effects on the intestinal microbial community; however, the metabolic landscape and anti-obesity mechanism remain unclear.
34784410	2	17	theme	microbiota	285:294	arg1	modulation					267:276	appropriate modulation	255:276	appropriate modulation of gut microbiota	255:294	Several studies showed that appropriate modulation of gut microbiota could help reshape the metabolic profile of obese individuals, thereby altering the development of obesity.
34784410	8	18	theme	Roseburia	1243:1251	arg1	enrichment					1209:1218	enrichment	1209:1218	enrichment of Bifidobacterium and Roseburia	1209:1251	Ce-RS3 increased the diversity of gut microbiota with enrichment of Bifidobacterium and Roseburia.
34784410	5	19	theme	metabolic	846:854	arg1	profiles					856:863	fecal metabolic profiles	840:863	fecal metabolic profiles	840:863	In the present study, obese mice were treated with Ce-RS3, and 16S rRNA gene sequencing and metabolomics were used to measure changes in gut microbiota and fecal metabolic profiles, respectively.
34784410	7	20	theme	intestinal	1100:1109	arg1	immunity					1111:1118	intestinal immunity	1100:1118	intestinal immunity	1100:1118	Ce-RS3 constructs a healthy gut microbiota structure and can enhance intestinal immunity and reduce metabolic inflammation.
34784410	4	21	from	edulis	504:509	arg1	fiber					533:537	a dietary fiber	523:537	a dietary fiber that exerts potential effects on the intestinal microbial community	523:605	Type 3 Resistant Starch from Canna edulis (Ce-RS3) is a dietary fiber that exerts potential effects on the intestinal microbial community; however, the metabolic landscape and anti-obesity mechanism remain unclear.
34784410	4	21	from	edulis	504:509	arg1	Starch					486:491	Type 3 Resistant Starch	469:491	Type 3 Resistant Starch from Canna edulis (Ce-RS3)	469:518	Type 3 Resistant Starch from Canna edulis (Ce-RS3) is a dietary fiber that exerts potential effects on the intestinal microbial community; however, the metabolic landscape and anti-obesity mechanism remain unclear.
34784410	9	22	from	metabolites	1343:1353	arg1	metabolism					1381:1390	amino acids and lipids metabolism	1358:1390	amino acids and lipids metabolism	1358:1390	Ce-RS3 regulated the systemic metabolic dysbiosis in obese mice and adjusted 26 abnormal metabolites in amino acids and lipids metabolism, many of which are related to the microbiome.
34784410	2	23	theme	gut	281:283	arg1	microbiota					285:294	gut microbiota	281:294	gut microbiota	281:294	Several studies showed that appropriate modulation of gut microbiota could help reshape the metabolic profile of obese individuals, thereby altering the development of obesity.
34784410	9	24	theme	obese	1307:1311	arg1	mice					1313:1316	obese mice	1307:1316	obese mice	1307:1316	Ce-RS3 regulated the systemic metabolic dysbiosis in obese mice and adjusted 26 abnormal metabolites in amino acids and lipids metabolism, many of which are related to the microbiome.
34784410	5	25	theme	present	691:697	arg1	study					699:703	the present study	687:703	the present study	687:703	In the present study, obese mice were treated with Ce-RS3, and 16S rRNA gene sequencing and metabolomics were used to measure changes in gut microbiota and fecal metabolic profiles, respectively.
34784410	2	26	theme	obesity	395:401	arg1	development					380:390	the development	376:390	the development of obesity	376:401	Several studies showed that appropriate modulation of gut microbiota could help reshape the metabolic profile of obese individuals, thereby altering the development of obesity.
34784410	0	27	theme	Type	0:3	arg1	starch					17:22	Type 3 resistant starch	0:22	Type 3 resistant starch from Canna edulis	0:40	Type 3 resistant starch from Canna edulis modulates obesity and obesity-related low-grade systemic inflammation in mice by regulating gut microbiota composition and metabolism.
34784410	6	28	theme	weight	937:942	arg1	gain					944:947	slow weight gain	932:947	slow weight gain in the mice	932:959	At the end of the treatment (13 weeks), we observed slow weight gain in the mice, and pathological damage and inflammation were substantially reduced.
34784410	7	29	theme	healthy	1051:1057	arg1	structure					1074:1082	a healthy gut microbiota structure	1049:1082	a healthy gut microbiota structure	1049:1082	Ce-RS3 constructs a healthy gut microbiota structure and can enhance intestinal immunity and reduce metabolic inflammation.
34784410	2	30	theme	metabolic	319:327	arg1	profile					329:335	the metabolic profile	315:335	the metabolic profile of obese individuals	315:356	Several studies showed that appropriate modulation of gut microbiota could help reshape the metabolic profile of obese individuals, thereby altering the development of obesity.
34784410	7	31	theme	metabolic	1131:1139	arg1	inflammation					1141:1152	metabolic inflammation	1131:1152	metabolic inflammation	1131:1152	Ce-RS3 constructs a healthy gut microbiota structure and can enhance intestinal immunity and reduce metabolic inflammation.
34784410	4	32	theme	potential	551:559	arg1	effects					561:567	potential effects	551:567	potential effects	551:567	Type 3 Resistant Starch from Canna edulis (Ce-RS3) is a dietary fiber that exerts potential effects on the intestinal microbial community; however, the metabolic landscape and anti-obesity mechanism remain unclear.
34784410	5	33	used	used	794:797	arg2	sequencing					761:770	16S rRNA gene sequencing	747:770	16S rRNA gene sequencing	747:770	In the present study, obese mice were treated with Ce-RS3, and 16S rRNA gene sequencing and metabolomics were used to measure changes in gut microbiota and fecal metabolic profiles, respectively.
34784410	5	33	used	used	794:797	arg2	metabolomics					776:787	metabolomics	776:787	metabolomics	776:787	In the present study, obese mice were treated with Ce-RS3, and 16S rRNA gene sequencing and metabolomics were used to measure changes in gut microbiota and fecal metabolic profiles, respectively.
34784410	8	34	theme	microbiota	1193:1202	arg1	diversity					1176:1184	the diversity	1172:1184	the diversity of gut microbiota	1172:1202	Ce-RS3 increased the diversity of gut microbiota with enrichment of Bifidobacterium and Roseburia.
34784410	7	35	theme	microbiota	1063:1072	arg1	structure					1074:1082	a healthy gut microbiota structure	1049:1082	a healthy gut microbiota structure	1049:1082	Ce-RS3 constructs a healthy gut microbiota structure and can enhance intestinal immunity and reduce metabolic inflammation.
34784410	1	36	theme	prevalent	195:203	arg1	Obesity					177:183	Obesity	177:183	Obesity	177:183	Obesity is a most prevalent human health problem.
34784410	1	36	theme	prevalent	195:203	arg1	problem					218:224	a most prevalent human health problem	188:224	a most prevalent human health problem	188:224	Obesity is a most prevalent human health problem.
34784410	0	37	theme	resistant	7:15	arg1	starch					17:22	Type 3 resistant starch	0:22	Type 3 resistant starch from Canna edulis	0:40	Type 3 resistant starch from Canna edulis modulates obesity and obesity-related low-grade systemic inflammation in mice by regulating gut microbiota composition and metabolism.
34784410	9	38	theme	metabolites	1343:1353	arg1	many					1393:1396	many	1393:1396	many	1393:1396	Ce-RS3 regulated the systemic metabolic dysbiosis in obese mice and adjusted 26 abnormal metabolites in amino acids and lipids metabolism, many of which are related to the microbiome.
34784410	9	38	theme	metabolites	1343:1353	arg1	metabolites					1343:1353	26 abnormal metabolites	1331:1353	26 abnormal metabolites	1331:1353	Ce-RS3 regulated the systemic metabolic dysbiosis in obese mice and adjusted 26 abnormal metabolites in amino acids and lipids metabolism, many of which are related to the microbiome.
34784410	10	39	theme	anti-obesity	1474:1485	arg1	effect					1487:1492	the anti-obesity effect	1470:1492	the anti-obesity effect of Ce-RS3	1470:1502	More importantly, we found that the anti-obesity effect of Ce-RS3 can be transferred by fecal transplantation.
34784410	11	40	theme	host-microbiota	1691:1705	arg1	interactions					1707:1718	host-microbiota interactions	1691:1718	host-microbiota interactions with impacts on the metabolome	1691:1749	The beneficial effects of Ce-RS3 might derive from gut microbiota changes, which might improve obesity and metabolic inflammation by altering host-microbiota interactions with impacts on the metabolome.
34784410	6	41	theme	slow	932:935	arg1	gain					944:947	slow weight gain	932:947	slow weight gain in the mice	932:959	At the end of the treatment (13 weeks), we observed slow weight gain in the mice, and pathological damage and inflammation were substantially reduced.
34784410	6	42	theme	treatment	898:906	arg1	end					887:889	the end	883:889	the end of the treatment (13 weeks)	883:917	At the end of the treatment (13 weeks), we observed slow weight gain in the mice, and pathological damage and inflammation were substantially reduced.
34784410	1	43	theme	human	205:209	arg1	Obesity					177:183	Obesity	177:183	Obesity	177:183	Obesity is a most prevalent human health problem.
34784410	1	43	theme	human	205:209	arg1	problem					218:224	a most prevalent human health problem	188:224	a most prevalent human health problem	188:224	Obesity is a most prevalent human health problem.
34784410	0	44	theme	Canna	29:33	arg1	edulis					35:40	Canna edulis	29:40	Canna edulis	29:40	Type 3 resistant starch from Canna edulis modulates obesity and obesity-related low-grade systemic inflammation in mice by regulating gut microbiota composition and metabolism.
34784410	0	45	theme	microbiota	138:147	arg1	composition					149:159	gut microbiota composition	134:159	gut microbiota composition	134:159	Type 3 resistant starch from Canna edulis modulates obesity and obesity-related low-grade systemic inflammation in mice by regulating gut microbiota composition and metabolism.
34784410	4	46	theme	Type	469:472	arg1	fiber					533:537	a dietary fiber	523:537	a dietary fiber that exerts potential effects on the intestinal microbial community	523:605	Type 3 Resistant Starch from Canna edulis (Ce-RS3) is a dietary fiber that exerts potential effects on the intestinal microbial community; however, the metabolic landscape and anti-obesity mechanism remain unclear.
34784410	4	46	theme	Type	469:472	arg1	Starch					486:491	Type 3 Resistant Starch	469:491	Type 3 Resistant Starch from Canna edulis (Ce-RS3)	469:518	Type 3 Resistant Starch from Canna edulis (Ce-RS3) is a dietary fiber that exerts potential effects on the intestinal microbial community; however, the metabolic landscape and anti-obesity mechanism remain unclear.
34784410	11	47	with	interactions	1707:1718	arg1	impacts					1725:1731	impacts	1725:1731	impacts on the metabolome	1725:1749	The beneficial effects of Ce-RS3 might derive from gut microbiota changes, which might improve obesity and metabolic inflammation by altering host-microbiota interactions with impacts on the metabolome.
34784410	1	48	theme	health	211:216	arg1	Obesity					177:183	Obesity	177:183	Obesity	177:183	Obesity is a most prevalent human health problem.
34784410	1	48	theme	health	211:216	arg1	problem					218:224	a most prevalent human health problem	188:224	a most prevalent human health problem	188:224	Obesity is a most prevalent human health problem.
34784410	8	49	theme	gut	1189:1191	arg1	microbiota					1193:1202	gut microbiota	1189:1202	gut microbiota	1189:1202	Ce-RS3 increased the diversity of gut microbiota with enrichment of Bifidobacterium and Roseburia.
34784410	9	50	theme	amino	1358:1362	arg1	acids					1364:1368	amino acids	1358:1368	amino acids	1358:1368	Ce-RS3 regulated the systemic metabolic dysbiosis in obese mice and adjusted 26 abnormal metabolites in amino acids and lipids metabolism, many of which are related to the microbiome.
34784410	0	51	theme	gut	134:136	arg1	composition					149:159	gut microbiota composition	134:159	gut microbiota composition	134:159	Type 3 resistant starch from Canna edulis modulates obesity and obesity-related low-grade systemic inflammation in mice by regulating gut microbiota composition and metabolism.
34784410	12	52	with	prebiotic	1791:1799	arg1	value					1816:1820	potential value	1806:1820	potential value for the treatment of obesity	1806:1849	In conclusion, Ce-RS3 can be used as a prebiotic with potential value for the treatment of obesity.
34784410	9	53	theme	abnormal	1334:1341	arg1	metabolites					1343:1353	26 abnormal metabolites	1331:1353	26 abnormal metabolites	1331:1353	Ce-RS3 regulated the systemic metabolic dysbiosis in obese mice and adjusted 26 abnormal metabolites in amino acids and lipids metabolism, many of which are related to the microbiome.
34784410	12	54	theme	potential	1806:1814	arg1	value					1816:1820	potential value	1806:1820	potential value for the treatment of obesity	1806:1849	In conclusion, Ce-RS3 can be used as a prebiotic with potential value for the treatment of obesity.
34784410	4	55	theme	anti-obesity	645:656	arg1	mechanism					658:666	the metabolic landscape and anti-obesity mechanism	617:666	the metabolic landscape and anti-obesity mechanism	617:666	Type 3 Resistant Starch from Canna edulis (Ce-RS3) is a dietary fiber that exerts potential effects on the intestinal microbial community; however, the metabolic landscape and anti-obesity mechanism remain unclear.
34784410	3	56	theme	nutritional	406:416	arg1	strategy					418:425	A nutritional strategy	404:425	A nutritional strategy for treating obesity	404:446	A nutritional strategy for treating obesity includes prebiotics.
34784410	4	57	theme	dietary	525:531	arg1	fiber					533:537	a dietary fiber	523:537	a dietary fiber that exerts potential effects on the intestinal microbial community	523:605	Type 3 Resistant Starch from Canna edulis (Ce-RS3) is a dietary fiber that exerts potential effects on the intestinal microbial community; however, the metabolic landscape and anti-obesity mechanism remain unclear.
34784410	4	57	theme	dietary	525:531	arg1	Starch					486:491	Type 3 Resistant Starch	469:491	Type 3 Resistant Starch from Canna edulis (Ce-RS3)	469:518	Type 3 Resistant Starch from Canna edulis (Ce-RS3) is a dietary fiber that exerts potential effects on the intestinal microbial community; however, the metabolic landscape and anti-obesity mechanism remain unclear.
34784410	0	58	from	obesity	52:58	arg1	mice					115:118	mice	115:118	mice	115:118	Type 3 resistant starch from Canna edulis modulates obesity and obesity-related low-grade systemic inflammation in mice by regulating gut microbiota composition and metabolism.
34784410	2	59	theme	Several	227:233	arg1	studies					235:241	Several studies	227:241	Several studies	227:241	Several studies showed that appropriate modulation of gut microbiota could help reshape the metabolic profile of obese individuals, thereby altering the development of obesity.
34784410	12	60	theme	obesity	1843:1849	arg1	treatment					1830:1838	the treatment	1826:1838	the treatment of obesity	1826:1849	In conclusion, Ce-RS3 can be used as a prebiotic with potential value for the treatment of obesity.
34784410	5	61	theme	obese	706:710	arg1	mice					712:715	obese mice	706:715	obese mice	706:715	In the present study, obese mice were treated with Ce-RS3, and 16S rRNA gene sequencing and metabolomics were used to measure changes in gut microbiota and fecal metabolic profiles, respectively.
34784410	6	62	from	gain	944:947	arg1	mice					956:959	the mice	952:959	the mice	952:959	At the end of the treatment (13 weeks), we observed slow weight gain in the mice, and pathological damage and inflammation were substantially reduced.
34784410	9	63	theme	metabolic	1284:1292	arg1	dysbiosis					1294:1302	the systemic metabolic dysbiosis	1271:1302	the systemic metabolic dysbiosis	1271:1302	Ce-RS3 regulated the systemic metabolic dysbiosis in obese mice and adjusted 26 abnormal metabolites in amino acids and lipids metabolism, many of which are related to the microbiome.
34784410	2	64	theme	individuals	346:356	arg1	profile					329:335	the metabolic profile	315:335	the metabolic profile of obese individuals	315:356	Several studies showed that appropriate modulation of gut microbiota could help reshape the metabolic profile of obese individuals, thereby altering the development of obesity.
34784410	9	65	theme	lipids	1374:1379	arg1	metabolism					1381:1390	amino acids and lipids metabolism	1358:1390	amino acids and lipids metabolism	1358:1390	Ce-RS3 regulated the systemic metabolic dysbiosis in obese mice and adjusted 26 abnormal metabolites in amino acids and lipids metabolism, many of which are related to the microbiome.
34784410	10	66	theme	Ce-RS3	1497:1502	arg1	effect					1487:1492	the anti-obesity effect	1470:1492	the anti-obesity effect of Ce-RS3	1470:1502	More importantly, we found that the anti-obesity effect of Ce-RS3 can be transferred by fecal transplantation.
34784410	11	67	theme	beneficial	1553:1562	arg1	effects					1564:1570	The beneficial effects	1549:1570	The beneficial effects of Ce-RS3	1549:1580	The beneficial effects of Ce-RS3 might derive from gut microbiota changes, which might improve obesity and metabolic inflammation by altering host-microbiota interactions with impacts on the metabolome.
34784410	11	68	theme	Ce-RS3	1575:1580	arg1	effects					1564:1570	The beneficial effects	1549:1570	The beneficial effects of Ce-RS3	1549:1580	The beneficial effects of Ce-RS3 might derive from gut microbiota changes, which might improve obesity and metabolic inflammation by altering host-microbiota interactions with impacts on the metabolome.
34784410	2	69	theme	obese	340:344	arg1	individuals					346:356	obese individuals	340:356	obese individuals	340:356	Several studies showed that appropriate modulation of gut microbiota could help reshape the metabolic profile of obese individuals, thereby altering the development of obesity.
34784410	11	70	theme	metabolic	1656:1664	arg1	inflammation					1666:1677	metabolic inflammation	1656:1677	metabolic inflammation	1656:1677	The beneficial effects of Ce-RS3 might derive from gut microbiota changes, which might improve obesity and metabolic inflammation by altering host-microbiota interactions with impacts on the metabolome.
34784410	0	71	from	inflammation	99:110	arg1	mice					115:118	mice	115:118	mice	115:118	Type 3 resistant starch from Canna edulis modulates obesity and obesity-related low-grade systemic inflammation in mice by regulating gut microbiota composition and metabolism.
34784410	11	72	theme	microbiota	1604:1613	arg1	changes					1615:1621	gut microbiota changes	1600:1621	gut microbiota changes	1600:1621	The beneficial effects of Ce-RS3 might derive from gut microbiota changes, which might improve obesity and metabolic inflammation by altering host-microbiota interactions with impacts on the metabolome.
34784410	6	73	theme	pathological	966:977	arg1	damage					979:984	pathological damage	966:984	pathological damage	966:984	At the end of the treatment (13 weeks), we observed slow weight gain in the mice, and pathological damage and inflammation were substantially reduced.
34784410	5	74	theme	16S	747:749	arg1	sequencing					761:770	16S rRNA gene sequencing	747:770	16S rRNA gene sequencing	747:770	In the present study, obese mice were treated with Ce-RS3, and 16S rRNA gene sequencing and metabolomics were used to measure changes in gut microbiota and fecal metabolic profiles, respectively.
34784410	5	75	theme	fecal	840:844	arg1	profiles					856:863	fecal metabolic profiles	840:863	fecal metabolic profiles	840:863	In the present study, obese mice were treated with Ce-RS3, and 16S rRNA gene sequencing and metabolomics were used to measure changes in gut microbiota and fecal metabolic profiles, respectively.
34784410	2	76	theme	appropriate	255:265	arg1	modulation					267:276	appropriate modulation	255:276	appropriate modulation of gut microbiota	255:294	Several studies showed that appropriate modulation of gut microbiota could help reshape the metabolic profile of obese individuals, thereby altering the development of obesity.
34784410	5	77	theme	gut	821:823	arg1	microbiota					825:834	gut microbiota	821:834	gut microbiota	821:834	In the present study, obese mice were treated with Ce-RS3, and 16S rRNA gene sequencing and metabolomics were used to measure changes in gut microbiota and fecal metabolic profiles, respectively.
34784410	4	78	theme	metabolic	621:629	arg1	mechanism					658:666	the metabolic landscape and anti-obesity mechanism	617:666	the metabolic landscape and anti-obesity mechanism	617:666	Type 3 Resistant Starch from Canna edulis (Ce-RS3) is a dietary fiber that exerts potential effects on the intestinal microbial community; however, the metabolic landscape and anti-obesity mechanism remain unclear.
34784410	5	79	theme	rRNA	751:754	arg1	sequencing					761:770	16S rRNA gene sequencing	747:770	16S rRNA gene sequencing	747:770	In the present study, obese mice were treated with Ce-RS3, and 16S rRNA gene sequencing and metabolomics were used to measure changes in gut microbiota and fecal metabolic profiles, respectively.
34784410	9	80	theme	systemic	1275:1282	arg1	dysbiosis					1294:1302	the systemic metabolic dysbiosis	1271:1302	the systemic metabolic dysbiosis	1271:1302	Ce-RS3 regulated the systemic metabolic dysbiosis in obese mice and adjusted 26 abnormal metabolites in amino acids and lipids metabolism, many of which are related to the microbiome.
34784410	8	81	theme	Bifidobacterium	1223:1237	arg1	enrichment					1209:1218	enrichment	1209:1218	enrichment of Bifidobacterium and Roseburia	1209:1251	Ce-RS3 increased the diversity of gut microbiota with enrichment of Bifidobacterium and Roseburia.
34784410	9	82	theme	acids	1364:1368	arg1	metabolism					1381:1390	amino acids and lipids metabolism	1358:1390	amino acids and lipids metabolism	1358:1390	Ce-RS3 regulated the systemic metabolic dysbiosis in obese mice and adjusted 26 abnormal metabolites in amino acids and lipids metabolism, many of which are related to the microbiome.
32315211	3	0	theme	underappreciated	505:520	arg1	role					522:525	a previously underappreciated role	492:525	a previously underappreciated role	492:525	Recent studies suggest that increased intake of dietary carbohydrates plays a previously underappreciated role in the promotion of obesity and consequent metabolic dysfunction.
32315211	7	1	from	alterations	1403:1413	arg1	islets					1331:1336	pancreatic islets	1320:1336	pancreatic islets highlighted by restricted capacity for β-cell mass expansion	1320:1397	Moreover, severe restriction of dietary carbohydrates also results in functional, morphological, and molecular changes in pancreatic islets highlighted by restricted capacity for β-cell mass expansion and alterations in insulin secretory response.
32315211	7	1	from	alterations	1403:1413	arg1	response					1436:1443	insulin secretory response	1418:1443	insulin secretory response	1418:1443	Moreover, severe restriction of dietary carbohydrates also results in functional, morphological, and molecular changes in pancreatic islets highlighted by restricted capacity for β-cell mass expansion and alterations in insulin secretory response.
32315211	8	2	from	biology	1623:1629	arg1	evaluation					1572:1581	further evaluation	1564:1581	further evaluation of the effects of these diets on β-cell biology in humans	1564:1639	These studies support the hypothesis that low-carbohydrate/high-fat diets provide antiobesogenic benefits and suggest further evaluation of the effects of these diets on β-cell biology in humans.
32315211	8	2	from	biology	1623:1629	arg1	humans					1634:1639	humans	1634:1639	humans	1634:1639	These studies support the hypothesis that low-carbohydrate/high-fat diets provide antiobesogenic benefits and suggest further evaluation of the effects of these diets on β-cell biology in humans.
32315211	5	3	from	fat	860:862	arg1	calories					846:853	>60% calories	841:853	>60% calories from fat	841:862	To address this, we exposed C57BL/6J mice to 12 wk of 3 eucaloric high-fat diets (>60% calories from fat) with varying total carbohydrate (1-20%) and sucrose (0-20%) content.
32315211	2	4	theme	metabolic	340:348	arg1	function					350:357	metabolic function	340:357	metabolic function	340:357	The pathogenesis of obesity and T2DM is influenced by alterations in diet macronutrient composition, which regulate energy expenditure, metabolic function, glucose homeostasis, and pancreatic islet cell biology.
32315211	8	5	from	evaluation	1572:1581	arg1	biology					1623:1629	β-cell biology	1616:1629	β-cell biology in humans	1616:1639	These studies support the hypothesis that low-carbohydrate/high-fat diets provide antiobesogenic benefits and suggest further evaluation of the effects of these diets on β-cell biology in humans.
32315211	6	6	theme	high-fat	1182:1189	arg1	diets					1191:1195	carbohydrate-rich, high-fat diets	1163:1195	diets	1191:1195	Our results show that severe restriction of dietary carbohydrates characteristic of ketogenic diets reduces body fat accumulation, enhances energy expenditure, and reduces prevailing glycemia and insulin resistance compared with carbohydrate-rich, high-fat diets.
32315211	2	7	theme	diet	273:276	arg1	composition					292:302	diet macronutrient composition	273:302	diet macronutrient composition	273:302	The pathogenesis of obesity and T2DM is influenced by alterations in diet macronutrient composition, which regulate energy expenditure, metabolic function, glucose homeostasis, and pancreatic islet cell biology.
32315211	3	8	theme	metabolic	570:578	arg1	dysfunction					580:590	consequent metabolic dysfunction	559:590	consequent metabolic dysfunction	559:590	Recent studies suggest that increased intake of dietary carbohydrates plays a previously underappreciated role in the promotion of obesity and consequent metabolic dysfunction.
32315211	7	9	theme	mass	1384:1387	arg1	expansion					1389:1397	β-cell mass expansion	1377:1397	β-cell mass expansion	1377:1397	Moreover, severe restriction of dietary carbohydrates also results in functional, morphological, and molecular changes in pancreatic islets highlighted by restricted capacity for β-cell mass expansion and alterations in insulin secretory response.
32315211	5	10	dep	carbohydrate	884:895	arg1	content					925:931	content	925:931	content	925:931	To address this, we exposed C57BL/6J mice to 12 wk of 3 eucaloric high-fat diets (>60% calories from fat) with varying total carbohydrate (1-20%) and sucrose (0-20%) content.
32315211	4	11	theme	islet	724:728	arg1	adaptions					730:738	energetic, metabolic, and islet adaptions	698:738	adaptions	730:738	Thus, in this study, we utilized mouse models to test the hypothesis that dietary carbohydrates modulate energetic, metabolic, and islet adaptions to high-fat diets.
32315211	7	12	from	changes	1309:1315	arg1	islets					1331:1336	pancreatic islets	1320:1336	pancreatic islets highlighted by restricted capacity for β-cell mass expansion	1320:1397	Moreover, severe restriction of dietary carbohydrates also results in functional, morphological, and molecular changes in pancreatic islets highlighted by restricted capacity for β-cell mass expansion and alterations in insulin secretory response.
32315211	7	12	from	changes	1309:1315	arg1	response					1436:1443	insulin secretory response	1418:1443	insulin secretory response	1418:1443	Moreover, severe restriction of dietary carbohydrates also results in functional, morphological, and molecular changes in pancreatic islets highlighted by restricted capacity for β-cell mass expansion and alterations in insulin secretory response.
32315211	5	13	theme	diets	834:838	arg1	wk					807:808	12 wk	804:808	12 wk of 3 eucaloric high-fat diets (>60% calories from fat)	804:863	To address this, we exposed C57BL/6J mice to 12 wk of 3 eucaloric high-fat diets (>60% calories from fat) with varying total carbohydrate (1-20%) and sucrose (0-20%) content.
32315211	5	14	theme	eucaloric	815:823	arg1	diets					834:838	3 eucaloric high-fat diets	813:838	3 eucaloric high-fat diets (>60% calories from fat)	813:863	To address this, we exposed C57BL/6J mice to 12 wk of 3 eucaloric high-fat diets (>60% calories from fat) with varying total carbohydrate (1-20%) and sucrose (0-20%) content.
32315211	6	15	theme	energy	1074:1079	arg1	expenditure					1081:1091	energy expenditure	1074:1091	energy expenditure	1074:1091	Our results show that severe restriction of dietary carbohydrates characteristic of ketogenic diets reduces body fat accumulation, enhances energy expenditure, and reduces prevailing glycemia and insulin resistance compared with carbohydrate-rich, high-fat diets.
32315211	4	16	theme	mouse	626:630	arg1	models					632:637	mouse models	626:637	mouse models	626:637	Thus, in this study, we utilized mouse models to test the hypothesis that dietary carbohydrates modulate energetic, metabolic, and islet adaptions to high-fat diets.
32315211	3	17	theme	Recent	416:421	arg1	studies					423:429	Recent studies	416:429	Recent studies	416:429	Recent studies suggest that increased intake of dietary carbohydrates plays a previously underappreciated role in the promotion of obesity and consequent metabolic dysfunction.
32315211	1	18	theme	type	171:174	arg1	T2DM					197:200	T2DM	197:200	T2DM	197:200	Obesity is associated with several chronic comorbidities, one of which is type 2 diabetes mellitus (T2DM).
32315211	1	18	theme	type	171:174	arg1	mellitus					187:194	type 2 diabetes mellitus	171:194	type 2 diabetes mellitus (T2DM)	171:201	Obesity is associated with several chronic comorbidities, one of which is type 2 diabetes mellitus (T2DM).
32315211	7	19	theme	secretory	1426:1434	arg1	response					1436:1443	insulin secretory response	1418:1443	insulin secretory response	1418:1443	Moreover, severe restriction of dietary carbohydrates also results in functional, morphological, and molecular changes in pancreatic islets highlighted by restricted capacity for β-cell mass expansion and alterations in insulin secretory response.
32315211	6	20	theme	diets	1028:1032	arg1	characteristic					1000:1013	characteristic	1000:1013	characteristic	1000:1013	Our results show that severe restriction of dietary carbohydrates characteristic of ketogenic diets reduces body fat accumulation, enhances energy expenditure, and reduces prevailing glycemia and insulin resistance compared with carbohydrate-rich, high-fat diets.
32315211	7	21	theme	molecular	1299:1307	arg1	changes					1309:1315	functional, morphological, and molecular changes	1268:1315	functional, morphological, and molecular changes in pancreatic islets highlighted by restricted capacity for β-cell mass expansion	1268:1397	Moreover, severe restriction of dietary carbohydrates also results in functional, morphological, and molecular changes in pancreatic islets highlighted by restricted capacity for β-cell mass expansion and alterations in insulin secretory response.
32315211	6	22	theme	ketogenic	1018:1026	arg1	diets					1028:1032	ketogenic diets	1018:1032	ketogenic diets	1018:1032	Our results show that severe restriction of dietary carbohydrates characteristic of ketogenic diets reduces body fat accumulation, enhances energy expenditure, and reduces prevailing glycemia and insulin resistance compared with carbohydrate-rich, high-fat diets.
32315211	3	23	theme	dysfunction	580:590	arg1	promotion					534:542	the promotion	530:542	the promotion of obesity and consequent metabolic dysfunction	530:590	Recent studies suggest that increased intake of dietary carbohydrates plays a previously underappreciated role in the promotion of obesity and consequent metabolic dysfunction.
32315211	6	24	theme	characteristic	1000:1013	arg1	restriction					963:973	severe restriction	956:973	severe restriction of dietary carbohydrates characteristic of ketogenic diets	956:1032	Our results show that severe restriction of dietary carbohydrates characteristic of ketogenic diets reduces body fat accumulation, enhances energy expenditure, and reduces prevailing glycemia and insulin resistance compared with carbohydrate-rich, high-fat diets.
32315211	1	25	theme	diabetes	178:185	arg1	T2DM					197:200	T2DM	197:200	T2DM	197:200	Obesity is associated with several chronic comorbidities, one of which is type 2 diabetes mellitus (T2DM).
32315211	1	25	theme	diabetes	178:185	arg1	mellitus					187:194	type 2 diabetes mellitus	171:194	type 2 diabetes mellitus (T2DM)	171:201	Obesity is associated with several chronic comorbidities, one of which is type 2 diabetes mellitus (T2DM).
32315211	0	26	theme	Dietary	0:6	arg1	carbohydrates					8:20	Dietary carbohydrates	0:20	Dietary carbohydrates	0:20	Dietary carbohydrates modulate metabolic and β-cell adaptation to high-fat diet-induced obesity.
32315211	8	27	from	effects	1590:1596	arg1	biology					1623:1629	β-cell biology	1616:1629	β-cell biology in humans	1616:1639	These studies support the hypothesis that low-carbohydrate/high-fat diets provide antiobesogenic benefits and suggest further evaluation of the effects of these diets on β-cell biology in humans.
32315211	5	28	theme	%	844:844	arg1	calories					846:853	>60% calories	841:853	>60% calories from fat	841:862	To address this, we exposed C57BL/6J mice to 12 wk of 3 eucaloric high-fat diets (>60% calories from fat) with varying total carbohydrate (1-20%) and sucrose (0-20%) content.
32315211	0	29	theme	metabolic	31:39	arg1	adaptation					52:61	metabolic and β-cell adaptation	31:61	metabolic and β-cell adaptation to high-fat diet-induced obesity	31:94	Dietary carbohydrates modulate metabolic and β-cell adaptation to high-fat diet-induced obesity.
32315211	8	30	theme	further	1564:1570	arg1	evaluation					1572:1581	further evaluation	1564:1581	further evaluation of the effects of these diets on β-cell biology in humans	1564:1639	These studies support the hypothesis that low-carbohydrate/high-fat diets provide antiobesogenic benefits and suggest further evaluation of the effects of these diets on β-cell biology in humans.
32315211	7	31	theme	severe	1208:1213	arg1	restriction					1215:1225	severe restriction	1208:1225	severe restriction of dietary carbohydrates	1208:1250	Moreover, severe restriction of dietary carbohydrates also results in functional, morphological, and molecular changes in pancreatic islets highlighted by restricted capacity for β-cell mass expansion and alterations in insulin secretory response.
32315211	3	32	theme	consequent	559:568	arg1	dysfunction					580:590	consequent metabolic dysfunction	559:590	consequent metabolic dysfunction	559:590	Recent studies suggest that increased intake of dietary carbohydrates plays a previously underappreciated role in the promotion of obesity and consequent metabolic dysfunction.
32315211	7	33	theme	restricted	1353:1362	arg1	capacity					1364:1371	restricted capacity	1353:1371	restricted capacity for β-cell mass expansion	1353:1397	Moreover, severe restriction of dietary carbohydrates also results in functional, morphological, and molecular changes in pancreatic islets highlighted by restricted capacity for β-cell mass expansion and alterations in insulin secretory response.
32315211	6	34	theme	dietary	978:984	arg1	carbohydrates					986:998	dietary carbohydrates	978:998	dietary carbohydrates	978:998	Our results show that severe restriction of dietary carbohydrates characteristic of ketogenic diets reduces body fat accumulation, enhances energy expenditure, and reduces prevailing glycemia and insulin resistance compared with carbohydrate-rich, high-fat diets.
32315211	7	35	theme	insulin	1418:1424	arg1	response					1436:1443	insulin secretory response	1418:1443	insulin secretory response	1418:1443	Moreover, severe restriction of dietary carbohydrates also results in functional, morphological, and molecular changes in pancreatic islets highlighted by restricted capacity for β-cell mass expansion and alterations in insulin secretory response.
32315211	8	36	theme	antiobesogenic	1528:1541	arg1	benefits					1543:1550	antiobesogenic benefits	1528:1550	antiobesogenic benefits	1528:1550	These studies support the hypothesis that low-carbohydrate/high-fat diets provide antiobesogenic benefits and suggest further evaluation of the effects of these diets on β-cell biology in humans.
32315211	4	37	used	utilized	617:624	arg2	we					614:615	we	614:615	we	614:615	Thus, in this study, we utilized mouse models to test the hypothesis that dietary carbohydrates modulate energetic, metabolic, and islet adaptions to high-fat diets.
32315211	6	38	theme	insulin	1130:1136	arg1	resistance					1138:1147	insulin resistance	1130:1147	insulin resistance	1130:1147	Our results show that severe restriction of dietary carbohydrates characteristic of ketogenic diets reduces body fat accumulation, enhances energy expenditure, and reduces prevailing glycemia and insulin resistance compared with carbohydrate-rich, high-fat diets.
32315211	2	39	theme	macronutrient	278:290	arg1	composition					292:302	diet macronutrient composition	273:302	diet macronutrient composition	273:302	The pathogenesis of obesity and T2DM is influenced by alterations in diet macronutrient composition, which regulate energy expenditure, metabolic function, glucose homeostasis, and pancreatic islet cell biology.
32315211	3	40	theme	dietary	464:470	arg1	carbohydrates					472:484	dietary carbohydrates	464:484	dietary carbohydrates	464:484	Recent studies suggest that increased intake of dietary carbohydrates plays a previously underappreciated role in the promotion of obesity and consequent metabolic dysfunction.
32315211	0	41	theme	β-cell	45:50	arg1	adaptation					52:61	metabolic and β-cell adaptation	31:61	metabolic and β-cell adaptation to high-fat diet-induced obesity	31:94	Dietary carbohydrates modulate metabolic and β-cell adaptation to high-fat diet-induced obesity.
32315211	4	42	theme	dietary	667:673	arg1	carbohydrates					675:687	dietary carbohydrates	667:687	dietary carbohydrates	667:687	Thus, in this study, we utilized mouse models to test the hypothesis that dietary carbohydrates modulate energetic, metabolic, and islet adaptions to high-fat diets.
32315211	3	43	theme	increased	444:452	arg1	intake					454:459	increased intake	444:459	increased intake of dietary carbohydrates	444:484	Recent studies suggest that increased intake of dietary carbohydrates plays a previously underappreciated role in the promotion of obesity and consequent metabolic dysfunction.
32315211	2	44	theme	obesity	224:230	arg1	pathogenesis					208:219	The pathogenesis	204:219	The pathogenesis of obesity and T2DM	204:239	The pathogenesis of obesity and T2DM is influenced by alterations in diet macronutrient composition, which regulate energy expenditure, metabolic function, glucose homeostasis, and pancreatic islet cell biology.
32315211	6	45	theme	carbohydrates	986:998	arg1	restriction					963:973	severe restriction	956:973	severe restriction of dietary carbohydrates characteristic of ketogenic diets	956:1032	Our results show that severe restriction of dietary carbohydrates characteristic of ketogenic diets reduces body fat accumulation, enhances energy expenditure, and reduces prevailing glycemia and insulin resistance compared with carbohydrate-rich, high-fat diets.
32315211	2	46	theme	cell	402:405	arg1	biology					407:413	pancreatic islet cell biology	385:413	pancreatic islet cell biology	385:413	The pathogenesis of obesity and T2DM is influenced by alterations in diet macronutrient composition, which regulate energy expenditure, metabolic function, glucose homeostasis, and pancreatic islet cell biology.
32315211	2	47	theme	pancreatic	385:394	arg1	biology					407:413	pancreatic islet cell biology	385:413	pancreatic islet cell biology	385:413	The pathogenesis of obesity and T2DM is influenced by alterations in diet macronutrient composition, which regulate energy expenditure, metabolic function, glucose homeostasis, and pancreatic islet cell biology.
32315211	5	48	theme	>60	841:843	arg1	%					844:844	%	844:844	%	844:844	To address this, we exposed C57BL/6J mice to 12 wk of 3 eucaloric high-fat diets (>60% calories from fat) with varying total carbohydrate (1-20%) and sucrose (0-20%) content.
32315211	2	49	theme	islet	396:400	arg1	biology					407:413	pancreatic islet cell biology	385:413	pancreatic islet cell biology	385:413	The pathogenesis of obesity and T2DM is influenced by alterations in diet macronutrient composition, which regulate energy expenditure, metabolic function, glucose homeostasis, and pancreatic islet cell biology.
32315211	8	50	theme	effects	1590:1596	arg1	evaluation					1572:1581	further evaluation	1564:1581	further evaluation of the effects of these diets on β-cell biology in humans	1564:1639	These studies support the hypothesis that low-carbohydrate/high-fat diets provide antiobesogenic benefits and suggest further evaluation of the effects of these diets on β-cell biology in humans.
32315211	6	51	theme	severe	956:961	arg1	restriction					963:973	severe restriction	956:973	severe restriction of dietary carbohydrates characteristic of ketogenic diets	956:1032	Our results show that severe restriction of dietary carbohydrates characteristic of ketogenic diets reduces body fat accumulation, enhances energy expenditure, and reduces prevailing glycemia and insulin resistance compared with carbohydrate-rich, high-fat diets.
32315211	5	52	dep	diets	834:838	arg1	calories					846:853	>60% calories	841:853	>60% calories from fat	841:862	To address this, we exposed C57BL/6J mice to 12 wk of 3 eucaloric high-fat diets (>60% calories from fat) with varying total carbohydrate (1-20%) and sucrose (0-20%) content.
32315211	6	53	theme	fat	1047:1049	arg1	accumulation					1051:1062	body fat accumulation	1042:1062	body fat accumulation	1042:1062	Our results show that severe restriction of dietary carbohydrates characteristic of ketogenic diets reduces body fat accumulation, enhances energy expenditure, and reduces prevailing glycemia and insulin resistance compared with carbohydrate-rich, high-fat diets.
32315211	1	54	theme	several	124:130	arg1	comorbidities					140:152	several chronic comorbidities	124:152	several chronic comorbidities	124:152	Obesity is associated with several chronic comorbidities, one of which is type 2 diabetes mellitus (T2DM).
32315211	5	55	theme	C57BL/6J	787:794	arg1	mice					796:799	C57BL/6J mice	787:799	C57BL/6J mice	787:799	To address this, we exposed C57BL/6J mice to 12 wk of 3 eucaloric high-fat diets (>60% calories from fat) with varying total carbohydrate (1-20%) and sucrose (0-20%) content.
32315211	8	56	theme	diets	1607:1611	arg1	effects					1590:1596	the effects	1586:1596	the effects of these diets on β-cell biology in humans	1586:1639	These studies support the hypothesis that low-carbohydrate/high-fat diets provide antiobesogenic benefits and suggest further evaluation of the effects of these diets on β-cell biology in humans.
32315211	3	57	theme	carbohydrates	472:484	arg1	intake					454:459	increased intake	444:459	increased intake of dietary carbohydrates	444:484	Recent studies suggest that increased intake of dietary carbohydrates plays a previously underappreciated role in the promotion of obesity and consequent metabolic dysfunction.
32315211	8	58	theme	β-cell	1616:1621	arg1	biology					1623:1629	β-cell biology	1616:1629	β-cell biology in humans	1616:1639	These studies support the hypothesis that low-carbohydrate/high-fat diets provide antiobesogenic benefits and suggest further evaluation of the effects of these diets on β-cell biology in humans.
32315211	7	59	theme	carbohydrates	1238:1250	arg1	restriction					1215:1225	severe restriction	1208:1225	severe restriction of dietary carbohydrates	1208:1250	Moreover, severe restriction of dietary carbohydrates also results in functional, morphological, and molecular changes in pancreatic islets highlighted by restricted capacity for β-cell mass expansion and alterations in insulin secretory response.
32315211	1	60	theme	chronic	132:138	arg1	comorbidities					140:152	several chronic comorbidities	124:152	several chronic comorbidities	124:152	Obesity is associated with several chronic comorbidities, one of which is type 2 diabetes mellitus (T2DM).
32315211	2	61	from	alterations	258:268	arg1	composition					292:302	diet macronutrient composition	273:302	diet macronutrient composition	273:302	The pathogenesis of obesity and T2DM is influenced by alterations in diet macronutrient composition, which regulate energy expenditure, metabolic function, glucose homeostasis, and pancreatic islet cell biology.
32315211	7	62	theme	pancreatic	1320:1329	arg1	islets					1331:1336	pancreatic islets	1320:1336	pancreatic islets highlighted by restricted capacity for β-cell mass expansion	1320:1397	Moreover, severe restriction of dietary carbohydrates also results in functional, morphological, and molecular changes in pancreatic islets highlighted by restricted capacity for β-cell mass expansion and alterations in insulin secretory response.
32315211	6	63	theme	carbohydrate-rich	1163:1179	arg1	diets					1191:1195	carbohydrate-rich, high-fat diets	1163:1195	diets	1191:1195	Our results show that severe restriction of dietary carbohydrates characteristic of ketogenic diets reduces body fat accumulation, enhances energy expenditure, and reduces prevailing glycemia and insulin resistance compared with carbohydrate-rich, high-fat diets.
32315211	1	64	theme	comorbidities	140:152	arg1	comorbidities					140:152	several chronic comorbidities	124:152	several chronic comorbidities	124:152	Obesity is associated with several chronic comorbidities, one of which is type 2 diabetes mellitus (T2DM).
32315211	1	64	theme	comorbidities	140:152	arg1	one					155:157	one	155:157	one	155:157	Obesity is associated with several chronic comorbidities, one of which is type 2 diabetes mellitus (T2DM).
32315211	7	65	theme	β-cell	1377:1382	arg1	expansion					1389:1397	β-cell mass expansion	1377:1397	β-cell mass expansion	1377:1397	Moreover, severe restriction of dietary carbohydrates also results in functional, morphological, and molecular changes in pancreatic islets highlighted by restricted capacity for β-cell mass expansion and alterations in insulin secretory response.
32315211	5	66	theme	total	878:882	arg1	%					902:902	1-20%	898:902	1-20%	898:902	To address this, we exposed C57BL/6J mice to 12 wk of 3 eucaloric high-fat diets (>60% calories from fat) with varying total carbohydrate (1-20%) and sucrose (0-20%) content.
32315211	5	66	theme	total	878:882	arg1	carbohydrate					884:895	total carbohydrate	878:895	total carbohydrate (1-20%)	878:903	To address this, we exposed C57BL/6J mice to 12 wk of 3 eucaloric high-fat diets (>60% calories from fat) with varying total carbohydrate (1-20%) and sucrose (0-20%) content.
32315211	3	67	theme	obesity	547:553	arg1	promotion					534:542	the promotion	530:542	the promotion of obesity and consequent metabolic dysfunction	530:590	Recent studies suggest that increased intake of dietary carbohydrates plays a previously underappreciated role in the promotion of obesity and consequent metabolic dysfunction.
32315211	4	68	theme	high-fat	743:750	arg1	diets					752:756	high-fat diets	743:756	high-fat diets	743:756	Thus, in this study, we utilized mouse models to test the hypothesis that dietary carbohydrates modulate energetic, metabolic, and islet adaptions to high-fat diets.
32315211	7	69	theme	dietary	1230:1236	arg1	carbohydrates					1238:1250	dietary carbohydrates	1230:1250	dietary carbohydrates	1230:1250	Moreover, severe restriction of dietary carbohydrates also results in functional, morphological, and molecular changes in pancreatic islets highlighted by restricted capacity for β-cell mass expansion and alterations in insulin secretory response.
32315211	7	70	theme	morphological	1280:1292	arg1	changes					1309:1315	functional, morphological, and molecular changes	1268:1315	functional, morphological, and molecular changes in pancreatic islets highlighted by restricted capacity for β-cell mass expansion	1268:1397	Moreover, severe restriction of dietary carbohydrates also results in functional, morphological, and molecular changes in pancreatic islets highlighted by restricted capacity for β-cell mass expansion and alterations in insulin secretory response.
32315211	2	71	theme	energy	320:325	arg1	expenditure					327:337	energy expenditure	320:337	energy expenditure	320:337	The pathogenesis of obesity and T2DM is influenced by alterations in diet macronutrient composition, which regulate energy expenditure, metabolic function, glucose homeostasis, and pancreatic islet cell biology.
32315211	0	72	theme	diet-induced	75:86	arg1	obesity					88:94	high-fat diet-induced obesity	66:94	high-fat diet-induced obesity	66:94	Dietary carbohydrates modulate metabolic and β-cell adaptation to high-fat diet-induced obesity.
32315211	5	73	theme	high-fat	825:832	arg1	diets					834:838	3 eucaloric high-fat diets	813:838	3 eucaloric high-fat diets (>60% calories from fat)	813:863	To address this, we exposed C57BL/6J mice to 12 wk of 3 eucaloric high-fat diets (>60% calories from fat) with varying total carbohydrate (1-20%) and sucrose (0-20%) content.
32315211	2	74	theme	T2DM	236:239	arg1	pathogenesis					208:219	The pathogenesis	204:219	The pathogenesis of obesity and T2DM	204:239	The pathogenesis of obesity and T2DM is influenced by alterations in diet macronutrient composition, which regulate energy expenditure, metabolic function, glucose homeostasis, and pancreatic islet cell biology.
32315211	7	75	theme	functional	1268:1277	arg1	changes					1309:1315	functional, morphological, and molecular changes	1268:1315	functional, morphological, and molecular changes in pancreatic islets highlighted by restricted capacity for β-cell mass expansion	1268:1397	Moreover, severe restriction of dietary carbohydrates also results in functional, morphological, and molecular changes in pancreatic islets highlighted by restricted capacity for β-cell mass expansion and alterations in insulin secretory response.
32315211	2	76	theme	glucose	360:366	arg1	homeostasis					368:378	glucose homeostasis	360:378	glucose homeostasis	360:378	The pathogenesis of obesity and T2DM is influenced by alterations in diet macronutrient composition, which regulate energy expenditure, metabolic function, glucose homeostasis, and pancreatic islet cell biology.
32315211	0	77	theme	high-fat	66:73	arg1	obesity					88:94	high-fat diet-induced obesity	66:94	high-fat diet-induced obesity	66:94	Dietary carbohydrates modulate metabolic and β-cell adaptation to high-fat diet-induced obesity.
32315211	8	78	theme	low-carbohydrate/high-fat	1488:1512	arg1	diets					1514:1518	low-carbohydrate/high-fat diets	1488:1518	low-carbohydrate/high-fat diets	1488:1518	These studies support the hypothesis that low-carbohydrate/high-fat diets provide antiobesogenic benefits and suggest further evaluation of the effects of these diets on β-cell biology in humans.
32315211	6	79	theme	body	1042:1045	arg1	fat					1047:1049	body fat	1042:1049	body fat accumulation	1042:1062	Our results show that severe restriction of dietary carbohydrates characteristic of ketogenic diets reduces body fat accumulation, enhances energy expenditure, and reduces prevailing glycemia and insulin resistance compared with carbohydrate-rich, high-fat diets.
33805641	0	0	theme	Low	71:73	arg1	Density					75:81	Novel Low Density	65:81	Novel Low Density Poly Ethylene/Chitosan/Basil Oil	65:114	Synthesis of a Novel Chitosan/Basil Oil Blend and Development of Novel Low Density Poly Ethylene/Chitosan/Basil Oil Active Packaging Films Following a Melt-Extrusion Process for Enhancing Chicken Breast Fillets Shelf-Life.
33805641	6	1	theme	packaging	896:904	arg1	properties					906:915	the best packaging properties	887:915	the best packaging properties	887:915	The film with 10% CS_BO content exhibited the best packaging properties, i.e., 33.0% higher tensile stress, 31.0% higher water barrier, 54.3% higher oxygen barrier, and 12.3% higher antioxidant activity values compared to the corresponding values of the LDPE films.
33805641	0	2	theme	Chicken	188:194	arg1	Shelf-Life					211:220	Enhancing Chicken Breast Fillets Shelf-Life	178:220	Enhancing Chicken Breast Fillets Shelf-Life	178:220	Synthesis of a Novel Chitosan/Basil Oil Blend and Development of Novel Low Density Poly Ethylene/Chitosan/Basil Oil Active Packaging Films Following a Melt-Extrusion Process for Enhancing Chicken Breast Fillets Shelf-Life.
33805641	6	3	theme	antioxidant	1027:1037	arg1	values					1048:1053	12.3% higher antioxidant activity values	1014:1053	12.3% higher antioxidant activity values	1014:1053	The film with 10% CS_BO content exhibited the best packaging properties, i.e., 33.0% higher tensile stress, 31.0% higher water barrier, 54.3% higher oxygen barrier, and 12.3% higher antioxidant activity values compared to the corresponding values of the LDPE films.
33805641	6	4	theme	higher	959:964	arg1	barrier					972:978	31.0% higher water barrier	953:978	31.0% higher water barrier	953:978	The film with 10% CS_BO content exhibited the best packaging properties, i.e., 33.0% higher tensile stress, 31.0% higher water barrier, 54.3% higher oxygen barrier, and 12.3% higher antioxidant activity values compared to the corresponding values of the LDPE films.
33805641	0	5	theme	Novel	65:69	arg1	Density					75:81	Novel Low Density	65:81	Novel Low Density Poly Ethylene/Chitosan/Basil Oil	65:114	Synthesis of a Novel Chitosan/Basil Oil Blend and Development of Novel Low Density Poly Ethylene/Chitosan/Basil Oil Active Packaging Films Following a Melt-Extrusion Process for Enhancing Chicken Breast Fillets Shelf-Life.
33805641	3	6	theme	evaporation/adsorption	647:668	arg1	process					670:676	a green evaporation/adsorption process	639:676	a green evaporation/adsorption process	639:676	The CS was modified to a CS_BO hydrophobic blend via a green evaporation/adsorption process.
33805641	0	7	theme	Enhancing	178:186	arg1	Shelf-Life					211:220	Enhancing Chicken Breast Fillets Shelf-Life	178:220	Enhancing Chicken Breast Fillets Shelf-Life	178:220	Synthesis of a Novel Chitosan/Basil Oil Blend and Development of Novel Low Density Poly Ethylene/Chitosan/Basil Oil Active Packaging Films Following a Melt-Extrusion Process for Enhancing Chicken Breast Fillets Shelf-Life.
33805641	1	8	theme	packaging	447:455	arg1	film					457:460	an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film	371:460	an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film	371:460	An innovative process for the adsorption of the hydrophobic Basil-Oil (BO) into the hydrophilic food byproduct chitosan (CS) and the development of an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film was investigated in this work.
33805641	6	9	theme	higher	1020:1025	arg1	values					1048:1053	12.3% higher antioxidant activity values	1014:1053	12.3% higher antioxidant activity values	1014:1053	The film with 10% CS_BO content exhibited the best packaging properties, i.e., 33.0% higher tensile stress, 31.0% higher water barrier, 54.3% higher oxygen barrier, and 12.3% higher antioxidant activity values compared to the corresponding values of the LDPE films.
33805641	2	10	theme	study	510:514	arg1	idea					497:500	The idea	493:500	The idea of this study	493:514	The idea of this study was the use of the BO as both a bioactive agent and a compatibilizer.
33805641	2	10	theme	study	510:514	arg1	use					524:526	the use	520:526	the use of the BO as both a bioactive agent and a compatibilizer	520:583	The idea of this study was the use of the BO as both a bioactive agent and a compatibilizer.
33805641	0	11	theme	Poly	83:86	arg1	Oil					112:114	Novel Low Density Poly Ethylene/Chitosan/Basil Oil	65:114	Novel Low Density Poly Ethylene/Chitosan/Basil Oil	65:114	Synthesis of a Novel Chitosan/Basil Oil Blend and Development of Novel Low Density Poly Ethylene/Chitosan/Basil Oil Active Packaging Films Following a Melt-Extrusion Process for Enhancing Chicken Breast Fillets Shelf-Life.
33805641	8	12	theme	pure	1406:1409	arg1	film					1416:1419	pure LDPE film	1406:1419	pure LDPE film	1406:1419	These values were found to be lower by around 41% and 45%, respectively, compared with the corresponding lipid oxidation values of pure LDPE film.
33805641	0	13	theme	Fillets	203:209	arg1	Shelf-Life					211:220	Enhancing Chicken Breast Fillets Shelf-Life	178:220	Enhancing Chicken Breast Fillets Shelf-Life	178:220	Synthesis of a Novel Chitosan/Basil Oil Blend and Development of Novel Low Density Poly Ethylene/Chitosan/Basil Oil Active Packaging Films Following a Melt-Extrusion Process for Enhancing Chicken Breast Fillets Shelf-Life.
33805641	1	14	theme	film	457:460	arg1	CS					344:345	CS	344:345	CS	344:345	An innovative process for the adsorption of the hydrophobic Basil-Oil (BO) into the hydrophilic food byproduct chitosan (CS) and the development of an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film was investigated in this work.
33805641	1	14	theme	film	457:460	arg1	chitosan					334:341	the hydrophilic food byproduct chitosan	303:341	the hydrophilic food byproduct chitosan (CS)	303:346	An innovative process for the adsorption of the hydrophobic Basil-Oil (BO) into the hydrophilic food byproduct chitosan (CS) and the development of an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film was investigated in this work.
33805641	1	14	theme	film	457:460	arg1	development					356:366	the development	352:366	the development of an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film	352:460	An innovative process for the adsorption of the hydrophobic Basil-Oil (BO) into the hydrophilic food byproduct chitosan (CS) and the development of an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film was investigated in this work.
33805641	7	15	theme	breast	1149:1154	arg1	fillets					1156:1162	chicken breast fillets	1141:1162	chicken breast fillets which were packaged under vacuum using this film	1141:1211	The lipid oxidation values of chicken breast fillets which were packaged under vacuum using this film were measured after seven and after fourteen days of storage.
33805641	1	16	theme	hydrophobic	271:281	arg1	BO					294:295	BO	294:295	BO	294:295	An innovative process for the adsorption of the hydrophobic Basil-Oil (BO) into the hydrophilic food byproduct chitosan (CS) and the development of an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film was investigated in this work.
33805641	1	16	theme	hydrophobic	271:281	arg1	Basil-Oil					283:291	the hydrophobic Basil-Oil	267:291	the hydrophobic Basil-Oil (BO)	267:296	An innovative process for the adsorption of the hydrophobic Basil-Oil (BO) into the hydrophilic food byproduct chitosan (CS) and the development of an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film was investigated in this work.
33805641	0	17	theme	Density	75:81	arg1	Oil					112:114	Novel Low Density Poly Ethylene/Chitosan/Basil Oil	65:114	Novel Low Density Poly Ethylene/Chitosan/Basil Oil	65:114	Synthesis of a Novel Chitosan/Basil Oil Blend and Development of Novel Low Density Poly Ethylene/Chitosan/Basil Oil Active Packaging Films Following a Melt-Extrusion Process for Enhancing Chicken Breast Fillets Shelf-Life.
33805641	0	18	theme	Breast	196:201	arg1	Shelf-Life					211:220	Enhancing Chicken Breast Fillets Shelf-Life	178:220	Enhancing Chicken Breast Fillets Shelf-Life	178:220	Synthesis of a Novel Chitosan/Basil Oil Blend and Development of Novel Low Density Poly Ethylene/Chitosan/Basil Oil Active Packaging Films Following a Melt-Extrusion Process for Enhancing Chicken Breast Fillets Shelf-Life.
33805641	1	19	theme	Basil-Oil	283:291	arg1	adsorption					253:262	the adsorption	249:262	the adsorption of the hydrophobic Basil-Oil (BO) into the hydrophilic food byproduct chitosan (CS) and the development of an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film	249:460	An innovative process for the adsorption of the hydrophobic Basil-Oil (BO) into the hydrophilic food byproduct chitosan (CS) and the development of an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film was investigated in this work.
33805641	0	20	theme	Oil	112:114	arg1	Development					50:60	Development	50:60	Development of Novel Low Density Poly Ethylene/Chitosan/Basil Oil	50:114	Synthesis of a Novel Chitosan/Basil Oil Blend and Development of Novel Low Density Poly Ethylene/Chitosan/Basil Oil Active Packaging Films Following a Melt-Extrusion Process for Enhancing Chicken Breast Fillets Shelf-Life.
33805641	0	20	theme	Oil	112:114	arg1	Blend					40:44	a Novel Chitosan/Basil Oil Blend	13:44	a Novel Chitosan/Basil Oil Blend	13:44	Synthesis of a Novel Chitosan/Basil Oil Blend and Development of Novel Low Density Poly Ethylene/Chitosan/Basil Oil Active Packaging Films Following a Melt-Extrusion Process for Enhancing Chicken Breast Fillets Shelf-Life.
33805641	8	21	theme	film	1416:1419	arg1	values					1396:1401	the corresponding lipid oxidation values	1362:1401	the corresponding lipid oxidation values of pure LDPE film	1362:1419	These values were found to be lower by around 41% and 45%, respectively, compared with the corresponding lipid oxidation values of pure LDPE film.
33805641	2	22	theme	BO	535:536	arg1	idea					497:500	The idea	493:500	The idea of this study	493:514	The idea of this study was the use of the BO as both a bioactive agent and a compatibilizer.
33805641	2	22	theme	BO	535:536	arg1	use					524:526	the use	520:526	the use of the BO as both a bioactive agent and a compatibilizer	520:583	The idea of this study was the use of the BO as both a bioactive agent and a compatibilizer.
33805641	6	23	theme	oxygen	994:999	arg1	barrier					1001:1007	54.3% higher oxygen barrier	981:1007	54.3% higher oxygen barrier	981:1007	The film with 10% CS_BO content exhibited the best packaging properties, i.e., 33.0% higher tensile stress, 31.0% higher water barrier, 54.3% higher oxygen barrier, and 12.3% higher antioxidant activity values compared to the corresponding values of the LDPE films.
33805641	4	24	theme	advanced	750:757	arg1	properties					759:768	advanced properties	750:768	advanced properties	750:768	This blend was incorporated directly in the LDPE to produce films with advanced properties.
33805641	6	25	with	film	849:852	arg1	content					869:875	10% CS_BO content	859:875	10% CS_BO content	859:875	The film with 10% CS_BO content exhibited the best packaging properties, i.e., 33.0% higher tensile stress, 31.0% higher water barrier, 54.3% higher oxygen barrier, and 12.3% higher antioxidant activity values compared to the corresponding values of the LDPE films.
33805641	0	26	theme	Ethylene/Chitosan/Basil	88:110	arg1	Oil					112:114	Novel Low Density Poly Ethylene/Chitosan/Basil Oil	65:114	Novel Low Density Poly Ethylene/Chitosan/Basil Oil	65:114	Synthesis of a Novel Chitosan/Basil Oil Blend and Development of Novel Low Density Poly Ethylene/Chitosan/Basil Oil Active Packaging Films Following a Melt-Extrusion Process for Enhancing Chicken Breast Fillets Shelf-Life.
33805641	6	27	theme	CS_BO	863:867	arg1	content					869:875	10% CS_BO content	859:875	10% CS_BO content	859:875	The film with 10% CS_BO content exhibited the best packaging properties, i.e., 33.0% higher tensile stress, 31.0% higher water barrier, 54.3% higher oxygen barrier, and 12.3% higher antioxidant activity values compared to the corresponding values of the LDPE films.
33805641	6	28	theme	films	1104:1108	arg1	values					1085:1090	the corresponding values	1067:1090	the corresponding values of the LDPE films	1067:1108	The film with 10% CS_BO content exhibited the best packaging properties, i.e., 33.0% higher tensile stress, 31.0% higher water barrier, 54.3% higher oxygen barrier, and 12.3% higher antioxidant activity values compared to the corresponding values of the LDPE films.
33805641	7	29	theme	oxidation	1121:1129	arg1	values					1131:1136	The lipid oxidation values	1111:1136	The lipid oxidation values of chicken breast fillets which were packaged under vacuum using this film	1111:1211	The lipid oxidation values of chicken breast fillets which were packaged under vacuum using this film were measured after seven and after fourteen days of storage.
33805641	5	30	theme	obtained	779:786	arg1	films					798:802	All the obtained composite films	771:802	All the obtained composite films	771:802	All the obtained composite films exhibited improved packaging properties.
33805641	6	31	theme	%	861:861	arg1	content					869:875	10% CS_BO content	859:875	10% CS_BO content	859:875	The film with 10% CS_BO content exhibited the best packaging properties, i.e., 33.0% higher tensile stress, 31.0% higher water barrier, 54.3% higher oxygen barrier, and 12.3% higher antioxidant activity values compared to the corresponding values of the LDPE films.
33805641	7	32	theme	fillets	1156:1162	arg1	values					1131:1136	The lipid oxidation values	1111:1136	The lipid oxidation values of chicken breast fillets which were packaged under vacuum using this film	1111:1211	The lipid oxidation values of chicken breast fillets which were packaged under vacuum using this film were measured after seven and after fourteen days of storage.
33805641	6	33	theme	LDPE	1099:1102	arg1	films					1104:1108	the LDPE films	1095:1108	the LDPE films	1095:1108	The film with 10% CS_BO content exhibited the best packaging properties, i.e., 33.0% higher tensile stress, 31.0% higher water barrier, 54.3% higher oxygen barrier, and 12.3% higher antioxidant activity values compared to the corresponding values of the LDPE films.
33805641	6	34	theme	higher	930:935	arg1	stress					945:950	33.0% higher tensile stress	924:950	33.0% higher tensile stress	924:950	The film with 10% CS_BO content exhibited the best packaging properties, i.e., 33.0% higher tensile stress, 31.0% higher water barrier, 54.3% higher oxygen barrier, and 12.3% higher antioxidant activity values compared to the corresponding values of the LDPE films.
33805641	5	35	theme	composite	788:796	arg1	films					798:802	All the obtained composite films	771:802	All the obtained composite films	771:802	All the obtained composite films exhibited improved packaging properties.
33805641	8	36	theme	lipid	1380:1384	arg1	values					1396:1401	the corresponding lipid oxidation values	1362:1401	the corresponding lipid oxidation values of pure LDPE film	1362:1419	These values were found to be lower by around 41% and 45%, respectively, compared with the corresponding lipid oxidation values of pure LDPE film.
33805641	8	37	theme	corresponding	1366:1378	arg1	values					1396:1401	the corresponding lipid oxidation values	1362:1401	the corresponding lipid oxidation values of pure LDPE film	1362:1419	These values were found to be lower by around 41% and 45%, respectively, compared with the corresponding lipid oxidation values of pure LDPE film.
33805641	3	38	theme	hydrophobic	617:627	arg1	blend					629:633	a CS_BO hydrophobic blend	609:633	a CS_BO hydrophobic blend	609:633	The CS was modified to a CS_BO hydrophobic blend via a green evaporation/adsorption process.
33805641	0	39	theme	Novel	15:19	arg1	Blend					40:44	a Novel Chitosan/Basil Oil Blend	13:44	a Novel Chitosan/Basil Oil Blend	13:44	Synthesis of a Novel Chitosan/Basil Oil Blend and Development of Novel Low Density Poly Ethylene/Chitosan/Basil Oil Active Packaging Films Following a Melt-Extrusion Process for Enhancing Chicken Breast Fillets Shelf-Life.
33805641	6	40	theme	tensile	937:943	arg1	stress					945:950	33.0% higher tensile stress	924:950	33.0% higher tensile stress	924:950	The film with 10% CS_BO content exhibited the best packaging properties, i.e., 33.0% higher tensile stress, 31.0% higher water barrier, 54.3% higher oxygen barrier, and 12.3% higher antioxidant activity values compared to the corresponding values of the LDPE films.
33805641	0	41	theme	Packaging	123:131	arg1	Films					133:137	Active Packaging Films	116:137	Active Packaging Films	116:137	Synthesis of a Novel Chitosan/Basil Oil Blend and Development of Novel Low Density Poly Ethylene/Chitosan/Basil Oil Active Packaging Films Following a Melt-Extrusion Process for Enhancing Chicken Breast Fillets Shelf-Life.
33805641	5	42	theme	improved	814:821	arg1	properties					833:842	improved packaging properties	814:842	improved packaging properties	814:842	All the obtained composite films exhibited improved packaging properties.
33805641	4	43	with	films	739:743	arg1	properties					759:768	advanced properties	750:768	advanced properties	750:768	This blend was incorporated directly in the LDPE to produce films with advanced properties.
33805641	0	44	theme	Active	116:121	arg1	Films					133:137	Active Packaging Films	116:137	Active Packaging Films	116:137	Synthesis of a Novel Chitosan/Basil Oil Blend and Development of Novel Low Density Poly Ethylene/Chitosan/Basil Oil Active Packaging Films Following a Melt-Extrusion Process for Enhancing Chicken Breast Fillets Shelf-Life.
33805641	3	45	theme	CS_BO	611:615	arg1	blend					629:633	a CS_BO hydrophobic blend	609:633	a CS_BO hydrophobic blend	609:633	The CS was modified to a CS_BO hydrophobic blend via a green evaporation/adsorption process.
33805641	5	46	theme	packaging	823:831	arg1	properties					833:842	improved packaging properties	814:842	improved packaging properties	814:842	All the obtained composite films exhibited improved packaging properties.
33805641	0	47	theme	Oil	36:38	arg1	Blend					40:44	a Novel Chitosan/Basil Oil Blend	13:44	a Novel Chitosan/Basil Oil Blend	13:44	Synthesis of a Novel Chitosan/Basil Oil Blend and Development of Novel Low Density Poly Ethylene/Chitosan/Basil Oil Active Packaging Films Following a Melt-Extrusion Process for Enhancing Chicken Breast Fillets Shelf-Life.
33805641	1	48	theme	advanced	374:381	arg1	film					457:460	an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film	371:460	an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film	371:460	An innovative process for the adsorption of the hydrophobic Basil-Oil (BO) into the hydrophilic food byproduct chitosan (CS) and the development of an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film was investigated in this work.
33805641	6	49	theme	higher	987:992	arg1	barrier					1001:1007	54.3% higher oxygen barrier	981:1007	54.3% higher oxygen barrier	981:1007	The film with 10% CS_BO content exhibited the best packaging properties, i.e., 33.0% higher tensile stress, 31.0% higher water barrier, 54.3% higher oxygen barrier, and 12.3% higher antioxidant activity values compared to the corresponding values of the LDPE films.
33805641	0	50	theme	Chitosan/Basil	21:34	arg1	Blend					40:44	a Novel Chitosan/Basil Oil Blend	13:44	a Novel Chitosan/Basil Oil Blend	13:44	Synthesis of a Novel Chitosan/Basil Oil Blend and Development of Novel Low Density Poly Ethylene/Chitosan/Basil Oil Active Packaging Films Following a Melt-Extrusion Process for Enhancing Chicken Breast Fillets Shelf-Life.
33805641	1	51	theme	low-density	383:393	arg1	film					457:460	an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film	371:460	an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film	371:460	An innovative process for the adsorption of the hydrophobic Basil-Oil (BO) into the hydrophilic food byproduct chitosan (CS) and the development of an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film was investigated in this work.
33805641	0	52	dep	Blend	40:44	arg1	Films					133:137	Active Packaging Films	116:137	Active Packaging Films	116:137	Synthesis of a Novel Chitosan/Basil Oil Blend and Development of Novel Low Density Poly Ethylene/Chitosan/Basil Oil Active Packaging Films Following a Melt-Extrusion Process for Enhancing Chicken Breast Fillets Shelf-Life.
33805641	1	53	theme	innovative	226:235	arg1	process					237:243	An innovative process	223:243	An innovative process for the adsorption of the hydrophobic Basil-Oil (BO) into the hydrophilic food byproduct chitosan (CS) and the development of an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film	223:460	An innovative process for the adsorption of the hydrophobic Basil-Oil (BO) into the hydrophilic food byproduct chitosan (CS) and the development of an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film was investigated in this work.
33805641	1	54	theme	hydrophilic	307:317	arg1	CS					344:345	CS	344:345	CS	344:345	An innovative process for the adsorption of the hydrophobic Basil-Oil (BO) into the hydrophilic food byproduct chitosan (CS) and the development of an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film was investigated in this work.
33805641	1	54	theme	hydrophilic	307:317	arg1	chitosan					334:341	the hydrophilic food byproduct chitosan	303:341	the hydrophilic food byproduct chitosan (CS)	303:346	An innovative process for the adsorption of the hydrophobic Basil-Oil (BO) into the hydrophilic food byproduct chitosan (CS) and the development of an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film was investigated in this work.
33805641	0	55	theme	Melt-Extrusion	151:164	arg1	Process					166:172	a Melt-Extrusion Process	149:172	a Melt-Extrusion Process for Enhancing Chicken Breast Fillets Shelf-Life	149:220	Synthesis of a Novel Chitosan/Basil Oil Blend and Development of Novel Low Density Poly Ethylene/Chitosan/Basil Oil Active Packaging Films Following a Melt-Extrusion Process for Enhancing Chicken Breast Fillets Shelf-Life.
33805641	1	56	theme	polyethylene/chitosan/basil-oil	395:425	arg1	film					457:460	an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film	371:460	an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film	371:460	An innovative process for the adsorption of the hydrophobic Basil-Oil (BO) into the hydrophilic food byproduct chitosan (CS) and the development of an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film was investigated in this work.
33805641	6	57	theme	10	859:860	arg1	%					861:861	%	861:861	%	861:861	The film with 10% CS_BO content exhibited the best packaging properties, i.e., 33.0% higher tensile stress, 31.0% higher water barrier, 54.3% higher oxygen barrier, and 12.3% higher antioxidant activity values compared to the corresponding values of the LDPE films.
33805641	6	58	theme	corresponding	1071:1083	arg1	values					1085:1090	the corresponding values	1067:1090	the corresponding values of the LDPE films	1067:1108	The film with 10% CS_BO content exhibited the best packaging properties, i.e., 33.0% higher tensile stress, 31.0% higher water barrier, 54.3% higher oxygen barrier, and 12.3% higher antioxidant activity values compared to the corresponding values of the LDPE films.
33805641	0	59	theme	Blend	40:44	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of a Novel Chitosan/Basil Oil Blend and Development of Novel Low Density Poly Ethylene/Chitosan/Basil Oil Active Packaging Films	0:137	Synthesis of a Novel Chitosan/Basil Oil Blend and Development of Novel Low Density Poly Ethylene/Chitosan/Basil Oil Active Packaging Films Following a Melt-Extrusion Process for Enhancing Chicken Breast Fillets Shelf-Life.
33805641	1	60	theme	food	319:322	arg1	CS					344:345	CS	344:345	CS	344:345	An innovative process for the adsorption of the hydrophobic Basil-Oil (BO) into the hydrophilic food byproduct chitosan (CS) and the development of an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film was investigated in this work.
33805641	1	60	theme	food	319:322	arg1	chitosan					334:341	the hydrophilic food byproduct chitosan	303:341	the hydrophilic food byproduct chitosan (CS)	303:346	An innovative process for the adsorption of the hydrophobic Basil-Oil (BO) into the hydrophilic food byproduct chitosan (CS) and the development of an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film was investigated in this work.
33805641	2	61	theme	bioactive	548:556	arg1	agent					558:562	a bioactive agent	546:562	a bioactive agent	546:562	The idea of this study was the use of the BO as both a bioactive agent and a compatibilizer.
33805641	6	62	theme	best	891:894	arg1	properties					906:915	the best packaging properties	887:915	the best packaging properties	887:915	The film with 10% CS_BO content exhibited the best packaging properties, i.e., 33.0% higher tensile stress, 31.0% higher water barrier, 54.3% higher oxygen barrier, and 12.3% higher antioxidant activity values compared to the corresponding values of the LDPE films.
33805641	3	63	theme	green	641:645	arg1	process					670:676	a green evaporation/adsorption process	639:676	a green evaporation/adsorption process	639:676	The CS was modified to a CS_BO hydrophobic blend via a green evaporation/adsorption process.
33805641	7	64	theme	chicken	1141:1147	arg1	fillets					1156:1162	chicken breast fillets	1141:1162	chicken breast fillets which were packaged under vacuum using this film	1141:1211	The lipid oxidation values of chicken breast fillets which were packaged under vacuum using this film were measured after seven and after fourteen days of storage.
33805641	1	65	theme	byproduct	324:332	arg1	CS					344:345	CS	344:345	CS	344:345	An innovative process for the adsorption of the hydrophobic Basil-Oil (BO) into the hydrophilic food byproduct chitosan (CS) and the development of an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film was investigated in this work.
33805641	1	65	theme	byproduct	324:332	arg1	chitosan					334:341	the hydrophilic food byproduct chitosan	303:341	the hydrophilic food byproduct chitosan (CS)	303:346	An innovative process for the adsorption of the hydrophobic Basil-Oil (BO) into the hydrophilic food byproduct chitosan (CS) and the development of an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film was investigated in this work.
33805641	1	66	theme	LDPE/CS_BO	428:437	arg1	film					457:460	an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film	371:460	an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film	371:460	An innovative process for the adsorption of the hydrophobic Basil-Oil (BO) into the hydrophilic food byproduct chitosan (CS) and the development of an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film was investigated in this work.
33805641	6	67	dep	stress	945:950	arg1	i.e.					918:921	i.e.	918:921	i.e.	918:921	The film with 10% CS_BO content exhibited the best packaging properties, i.e., 33.0% higher tensile stress, 31.0% higher water barrier, 54.3% higher oxygen barrier, and 12.3% higher antioxidant activity values compared to the corresponding values of the LDPE films.
33805641	8	68	theme	oxidation	1386:1394	arg1	values					1396:1401	the corresponding lipid oxidation values	1362:1401	the corresponding lipid oxidation values of pure LDPE film	1362:1419	These values were found to be lower by around 41% and 45%, respectively, compared with the corresponding lipid oxidation values of pure LDPE film.
33805641	8	69	theme	LDPE	1411:1414	arg1	film					1416:1419	pure LDPE film	1406:1419	pure LDPE film	1406:1419	These values were found to be lower by around 41% and 45%, respectively, compared with the corresponding lipid oxidation values of pure LDPE film.
33805641	1	70	theme	active	440:445	arg1	film					457:460	an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film	371:460	an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film	371:460	An innovative process for the adsorption of the hydrophobic Basil-Oil (BO) into the hydrophilic food byproduct chitosan (CS) and the development of an advanced low-density polyethylene/chitosan/basil-oil (LDPE/CS_BO) active packaging film was investigated in this work.
33805641	7	71	theme	storage	1266:1272	arg1	days					1258:1261	fourteen days	1249:1261	fourteen days of storage	1249:1272	The lipid oxidation values of chicken breast fillets which were packaged under vacuum using this film were measured after seven and after fourteen days of storage.
33805641	0	72	theme	Development	50:60	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of a Novel Chitosan/Basil Oil Blend and Development of Novel Low Density Poly Ethylene/Chitosan/Basil Oil Active Packaging Films	0:137	Synthesis of a Novel Chitosan/Basil Oil Blend and Development of Novel Low Density Poly Ethylene/Chitosan/Basil Oil Active Packaging Films Following a Melt-Extrusion Process for Enhancing Chicken Breast Fillets Shelf-Life.
33805641	6	73	theme	activity	1039:1046	arg1	values					1048:1053	12.3% higher antioxidant activity values	1014:1053	12.3% higher antioxidant activity values	1014:1053	The film with 10% CS_BO content exhibited the best packaging properties, i.e., 33.0% higher tensile stress, 31.0% higher water barrier, 54.3% higher oxygen barrier, and 12.3% higher antioxidant activity values compared to the corresponding values of the LDPE films.
33805641	7	74	theme	lipid	1115:1119	arg1	values					1131:1136	The lipid oxidation values	1111:1136	The lipid oxidation values of chicken breast fillets which were packaged under vacuum using this film	1111:1211	The lipid oxidation values of chicken breast fillets which were packaged under vacuum using this film were measured after seven and after fourteen days of storage.
33805641	6	75	theme	water	966:970	arg1	barrier					972:978	31.0% higher water barrier	953:978	31.0% higher water barrier	953:978	The film with 10% CS_BO content exhibited the best packaging properties, i.e., 33.0% higher tensile stress, 31.0% higher water barrier, 54.3% higher oxygen barrier, and 12.3% higher antioxidant activity values compared to the corresponding values of the LDPE films.
31859471	6	0	from	improvement	1128:1138	arg1	terms					1167:1171	terms	1167:1171	terms of echocardiographical, histological, and angiogenic outcomes	1167:1233	Compared with a single-mode system, injecting the HA-CHO/HHA hydrogel intramyocardially followed by painting a conductive GelDA/DA-PPy hydrogel patch on the heart surface results in a more pronounced improvement of the cardiac function in terms of echocardiographical, histological, and angiogenic outcomes.
31859471	6	1	theme	pronounced	1117:1126	arg1	improvement					1128:1138	a more pronounced improvement	1110:1138	a more pronounced improvement of the cardiac function in terms of echocardiographical, histological, and angiogenic outcomes	1110:1233	Compared with a single-mode system, injecting the HA-CHO/HHA hydrogel intramyocardially followed by painting a conductive GelDA/DA-PPy hydrogel patch on the heart surface results in a more pronounced improvement of the cardiac function in terms of echocardiographical, histological, and angiogenic outcomes.
31859471	6	2	theme	heart	1085:1089	arg1	surface					1091:1097	the heart surface	1081:1097	the heart surface	1081:1097	Compared with a single-mode system, injecting the HA-CHO/HHA hydrogel intramyocardially followed by painting a conductive GelDA/DA-PPy hydrogel patch on the heart surface results in a more pronounced improvement of the cardiac function in terms of echocardiographical, histological, and angiogenic outcomes.
31859471	0	3	theme	Myocardial	94:103	arg1	Infarction					105:114	Myocardial Infarction	94:114	Myocardial Infarction	94:114	Coadministration of an Adhesive Conductive Hydrogel Patch and an Injectable Hydrogel to Treat Myocardial Infarction.
31859471	6	4	theme	angiogenic	1215:1224	arg1	outcomes					1226:1233	echocardiographical, histological, and angiogenic outcomes	1176:1233	echocardiographical, histological, and angiogenic outcomes	1176:1233	Compared with a single-mode system, injecting the HA-CHO/HHA hydrogel intramyocardially followed by painting a conductive GelDA/DA-PPy hydrogel patch on the heart surface results in a more pronounced improvement of the cardiac function in terms of echocardiographical, histological, and angiogenic outcomes.
31859471	1	5	theme	myocardial	271:280	arg1	infarction					282:291	myocardial infarction	271:291	myocardial infarction	271:291	Over the past decade, tissue-engineering strategies, mainly involving injectable hydrogels and epicardial biomaterial patches, have been pursued to treat myocardial infarction.
31859471	5	6	theme	injectable	762:771	arg1	hydrogel					787:794	The injectable and cleavable hydrogel	758:794	The injectable and cleavable hydrogel	758:794	The injectable and cleavable hydrogel is formed in situ via a Schiff base reaction between oxidized sodium hyaluronic acid (HA-CHO) and hydrazided hyaluronic acid (HHA).
31859471	4	7	theme	GelDA	656:660	arg1	conjugates					663:672	dopamine-gelatin (GelDA) conjugates	638:672	dopamine-gelatin (GelDA) conjugates	638:672	The self-adhesive conductive hydrogel patch is fabricated based on Fe3+-induced ionic coordination between dopamine-gelatin (GelDA) conjugates and dopamine-functionalized polypyrrole (DA-PPy), which form a homogeneous network.
31859471	6	8	theme	single-mode	944:954	arg1	system					956:961	a single-mode system	942:961	a single-mode system	942:961	Compared with a single-mode system, injecting the HA-CHO/HHA hydrogel intramyocardially followed by painting a conductive GelDA/DA-PPy hydrogel patch on the heart surface results in a more pronounced improvement of the cardiac function in terms of echocardiographical, histological, and angiogenic outcomes.
31859471	4	9	theme	Fe3+-induced	598:609	arg1	coordination					617:628	Fe3+-induced ionic coordination	598:628	Fe3+-induced ionic coordination between dopamine-gelatin (GelDA) conjugates and dopamine-functionalized polypyrrole (DA-PPy), which form a homogeneous network	598:755	The self-adhesive conductive hydrogel patch is fabricated based on Fe3+-induced ionic coordination between dopamine-gelatin (GelDA) conjugates and dopamine-functionalized polypyrrole (DA-PPy), which form a homogeneous network.
31859471	2	10	theme	single	356:361	arg1	means					363:367	a single means	354:367	a single means	354:367	However, only limited therapeutic efficacy is achieved with a single means.
31859471	5	11	theme	hyaluronic	865:874	arg1	acid					876:879	oxidized sodium hyaluronic acid	849:879	oxidized sodium hyaluronic acid (HA-CHO)	849:888	The injectable and cleavable hydrogel is formed in situ via a Schiff base reaction between oxidized sodium hyaluronic acid (HA-CHO) and hydrazided hyaluronic acid (HHA).
31859471	5	11	theme	hyaluronic	865:874	arg1	HA-CHO					882:887	HA-CHO	882:887	HA-CHO	882:887	The injectable and cleavable hydrogel is formed in situ via a Schiff base reaction between oxidized sodium hyaluronic acid (HA-CHO) and hydrazided hyaluronic acid (HHA).
31859471	3	12	theme	hydrogel	463:470	arg1	patch					472:476	a conductive hydrogel patch	450:476	a conductive hydrogel patch	450:476	Here, a combined therapy approach is proposed, that is, the coadministration of a conductive hydrogel patch and injectable hydrogel to the infarcted myocardium.
31859471	5	13	theme	Schiff	820:825	arg1	reaction					832:839	a Schiff base reaction	818:839	a Schiff base reaction between oxidized sodium hyaluronic acid (HA-CHO) and hydrazided hyaluronic acid (HHA)	818:925	The injectable and cleavable hydrogel is formed in situ via a Schiff base reaction between oxidized sodium hyaluronic acid (HA-CHO) and hydrazided hyaluronic acid (HHA).
31859471	5	14	theme	base	827:830	arg1	reaction					832:839	a Schiff base reaction	818:839	a Schiff base reaction between oxidized sodium hyaluronic acid (HA-CHO) and hydrazided hyaluronic acid (HHA)	818:925	The injectable and cleavable hydrogel is formed in situ via a Schiff base reaction between oxidized sodium hyaluronic acid (HA-CHO) and hydrazided hyaluronic acid (HHA).
31859471	6	15	theme	GelDA/DA-PPy	1050:1061	arg1	patch					1072:1076	a conductive GelDA/DA-PPy hydrogel patch	1037:1076	a conductive GelDA/DA-PPy hydrogel patch on the heart surface	1037:1097	Compared with a single-mode system, injecting the HA-CHO/HHA hydrogel intramyocardially followed by painting a conductive GelDA/DA-PPy hydrogel patch on the heart surface results in a more pronounced improvement of the cardiac function in terms of echocardiographical, histological, and angiogenic outcomes.
31859471	4	16	theme	dopamine-functionalized	678:700	arg1	polypyrrole					702:712	dopamine-functionalized polypyrrole	678:712	dopamine-functionalized polypyrrole (DA-PPy)	678:721	The self-adhesive conductive hydrogel patch is fabricated based on Fe3+-induced ionic coordination between dopamine-gelatin (GelDA) conjugates and dopamine-functionalized polypyrrole (DA-PPy), which form a homogeneous network.
31859471	4	16	theme	dopamine-functionalized	678:700	arg1	DA-PPy					715:720	DA-PPy	715:720	DA-PPy	715:720	The self-adhesive conductive hydrogel patch is fabricated based on Fe3+-induced ionic coordination between dopamine-gelatin (GelDA) conjugates and dopamine-functionalized polypyrrole (DA-PPy), which form a homogeneous network.
31859471	3	17	theme	therapy	387:393	arg1	approach					395:402	a combined therapy approach	376:402	a combined therapy approach	376:402	Here, a combined therapy approach is proposed, that is, the coadministration of a conductive hydrogel patch and injectable hydrogel to the infarcted myocardium.
31859471	6	18	from	patch	1072:1076	arg1	surface					1091:1097	the heart surface	1081:1097	the heart surface	1081:1097	Compared with a single-mode system, injecting the HA-CHO/HHA hydrogel intramyocardially followed by painting a conductive GelDA/DA-PPy hydrogel patch on the heart surface results in a more pronounced improvement of the cardiac function in terms of echocardiographical, histological, and angiogenic outcomes.
31859471	1	19	theme	injectable	187:196	arg1	hydrogels					198:206	injectable hydrogels	187:206	injectable hydrogels	187:206	Over the past decade, tissue-engineering strategies, mainly involving injectable hydrogels and epicardial biomaterial patches, have been pursued to treat myocardial infarction.
31859471	6	20	theme	echocardiographical	1176:1194	arg1	outcomes					1226:1233	echocardiographical, histological, and angiogenic outcomes	1176:1233	echocardiographical, histological, and angiogenic outcomes	1176:1233	Compared with a single-mode system, injecting the HA-CHO/HHA hydrogel intramyocardially followed by painting a conductive GelDA/DA-PPy hydrogel patch on the heart surface results in a more pronounced improvement of the cardiac function in terms of echocardiographical, histological, and angiogenic outcomes.
31859471	5	21	theme	hydrazided	894:903	arg1	HHA					922:924	HHA	922:924	HHA	922:924	The injectable and cleavable hydrogel is formed in situ via a Schiff base reaction between oxidized sodium hyaluronic acid (HA-CHO) and hydrazided hyaluronic acid (HHA).
31859471	5	21	theme	hydrazided	894:903	arg1	acid					916:919	hydrazided hyaluronic acid	894:919	hydrazided hyaluronic acid (HHA)	894:925	The injectable and cleavable hydrogel is formed in situ via a Schiff base reaction between oxidized sodium hyaluronic acid (HA-CHO) and hydrazided hyaluronic acid (HHA).
31859471	0	22	theme	Adhesive	23:30	arg1	Patch					52:56	an Adhesive Conductive Hydrogel Patch	20:56	an Adhesive Conductive Hydrogel Patch	20:56	Coadministration of an Adhesive Conductive Hydrogel Patch and an Injectable Hydrogel to Treat Myocardial Infarction.
31859471	4	23	theme	hydrogel	560:567	arg1	patch					569:573	The self-adhesive conductive hydrogel patch	531:573	The self-adhesive conductive hydrogel patch	531:573	The self-adhesive conductive hydrogel patch is fabricated based on Fe3+-induced ionic coordination between dopamine-gelatin (GelDA) conjugates and dopamine-functionalized polypyrrole (DA-PPy), which form a homogeneous network.
31859471	2	24	theme	therapeutic	316:326	arg1	efficacy					328:335	only limited therapeutic efficacy	303:335	only limited therapeutic efficacy	303:335	However, only limited therapeutic efficacy is achieved with a single means.
31859471	6	25	from	terms	1167:1171	arg1	improvement					1128:1138	a more pronounced improvement	1110:1138	a more pronounced improvement of the cardiac function in terms of echocardiographical, histological, and angiogenic outcomes	1110:1233	Compared with a single-mode system, injecting the HA-CHO/HHA hydrogel intramyocardially followed by painting a conductive GelDA/DA-PPy hydrogel patch on the heart surface results in a more pronounced improvement of the cardiac function in terms of echocardiographical, histological, and angiogenic outcomes.
31859471	3	26	theme	conductive	452:461	arg1	patch					472:476	a conductive hydrogel patch	450:476	a conductive hydrogel patch	450:476	Here, a combined therapy approach is proposed, that is, the coadministration of a conductive hydrogel patch and injectable hydrogel to the infarcted myocardium.
31859471	3	27	theme	combined	378:385	arg1	approach					395:402	a combined therapy approach	376:402	a combined therapy approach	376:402	Here, a combined therapy approach is proposed, that is, the coadministration of a conductive hydrogel patch and injectable hydrogel to the infarcted myocardium.
31859471	2	28	theme	limited	308:314	arg1	efficacy					328:335	only limited therapeutic efficacy	303:335	only limited therapeutic efficacy	303:335	However, only limited therapeutic efficacy is achieved with a single means.
31859471	1	29	theme	epicardial	212:221	arg1	patches					235:241	epicardial biomaterial patches	212:241	epicardial biomaterial patches	212:241	Over the past decade, tissue-engineering strategies, mainly involving injectable hydrogels and epicardial biomaterial patches, have been pursued to treat myocardial infarction.
31859471	0	30	theme	Hydrogel	43:50	arg1	Patch					52:56	an Adhesive Conductive Hydrogel Patch	20:56	an Adhesive Conductive Hydrogel Patch	20:56	Coadministration of an Adhesive Conductive Hydrogel Patch and an Injectable Hydrogel to Treat Myocardial Infarction.
31859471	6	31	theme	outcomes	1226:1233	arg1	terms					1167:1171	terms	1167:1171	terms of echocardiographical, histological, and angiogenic outcomes	1167:1233	Compared with a single-mode system, injecting the HA-CHO/HHA hydrogel intramyocardially followed by painting a conductive GelDA/DA-PPy hydrogel patch on the heart surface results in a more pronounced improvement of the cardiac function in terms of echocardiographical, histological, and angiogenic outcomes.
31859471	1	32	theme	biomaterial	223:233	arg1	patches					235:241	epicardial biomaterial patches	212:241	epicardial biomaterial patches	212:241	Over the past decade, tissue-engineering strategies, mainly involving injectable hydrogels and epicardial biomaterial patches, have been pursued to treat myocardial infarction.
31859471	0	33	theme	Conductive	32:41	arg1	Patch					52:56	an Adhesive Conductive Hydrogel Patch	20:56	an Adhesive Conductive Hydrogel Patch	20:56	Coadministration of an Adhesive Conductive Hydrogel Patch and an Injectable Hydrogel to Treat Myocardial Infarction.
31859471	5	34	theme	cleavable	777:785	arg1	hydrogel					787:794	The injectable and cleavable hydrogel	758:794	The injectable and cleavable hydrogel	758:794	The injectable and cleavable hydrogel is formed in situ via a Schiff base reaction between oxidized sodium hyaluronic acid (HA-CHO) and hydrazided hyaluronic acid (HHA).
31859471	5	35	theme	hyaluronic	905:914	arg1	HHA					922:924	HHA	922:924	HHA	922:924	The injectable and cleavable hydrogel is formed in situ via a Schiff base reaction between oxidized sodium hyaluronic acid (HA-CHO) and hydrazided hyaluronic acid (HHA).
31859471	5	35	theme	hyaluronic	905:914	arg1	acid					916:919	hydrazided hyaluronic acid	894:919	hydrazided hyaluronic acid (HHA)	894:925	The injectable and cleavable hydrogel is formed in situ via a Schiff base reaction between oxidized sodium hyaluronic acid (HA-CHO) and hydrazided hyaluronic acid (HHA).
31859471	6	36	theme	HA-CHO/HHA	978:987	arg1	hydrogel					989:996	the HA-CHO/HHA hydrogel	974:996	the HA-CHO/HHA hydrogel intramyocardially followed by painting	974:1035	Compared with a single-mode system, injecting the HA-CHO/HHA hydrogel intramyocardially followed by painting a conductive GelDA/DA-PPy hydrogel patch on the heart surface results in a more pronounced improvement of the cardiac function in terms of echocardiographical, histological, and angiogenic outcomes.
31859471	6	37	theme	cardiac	1147:1153	arg1	function					1155:1162	the cardiac function	1143:1162	the cardiac function in terms of echocardiographical, histological, and angiogenic outcomes	1143:1233	Compared with a single-mode system, injecting the HA-CHO/HHA hydrogel intramyocardially followed by painting a conductive GelDA/DA-PPy hydrogel patch on the heart surface results in a more pronounced improvement of the cardiac function in terms of echocardiographical, histological, and angiogenic outcomes.
31859471	3	38	theme	injectable	482:491	arg1	hydrogel					493:500	injectable hydrogel	482:500	injectable hydrogel to the infarcted myocardium	482:528	Here, a combined therapy approach is proposed, that is, the coadministration of a conductive hydrogel patch and injectable hydrogel to the infarcted myocardium.
31859471	0	39	theme	Patch	52:56	arg1	Hydrogel					76:83	an Injectable Hydrogel	62:83	an Injectable Hydrogel	62:83	Coadministration of an Adhesive Conductive Hydrogel Patch and an Injectable Hydrogel to Treat Myocardial Infarction.
31859471	0	39	theme	Patch	52:56	arg1	Coadministration					0:15	Coadministration	0:15	Coadministration of an Adhesive Conductive Hydrogel Patch	0:56	Coadministration of an Adhesive Conductive Hydrogel Patch and an Injectable Hydrogel to Treat Myocardial Infarction.
31859471	4	40	theme	conductive	549:558	arg1	patch					569:573	The self-adhesive conductive hydrogel patch	531:573	The self-adhesive conductive hydrogel patch	531:573	The self-adhesive conductive hydrogel patch is fabricated based on Fe3+-induced ionic coordination between dopamine-gelatin (GelDA) conjugates and dopamine-functionalized polypyrrole (DA-PPy), which form a homogeneous network.
31859471	6	41	theme	function	1155:1162	arg1	improvement					1128:1138	a more pronounced improvement	1110:1138	a more pronounced improvement of the cardiac function in terms of echocardiographical, histological, and angiogenic outcomes	1110:1233	Compared with a single-mode system, injecting the HA-CHO/HHA hydrogel intramyocardially followed by painting a conductive GelDA/DA-PPy hydrogel patch on the heart surface results in a more pronounced improvement of the cardiac function in terms of echocardiographical, histological, and angiogenic outcomes.
31859471	4	42	theme	self-adhesive	535:547	arg1	patch					569:573	The self-adhesive conductive hydrogel patch	531:573	The self-adhesive conductive hydrogel patch	531:573	The self-adhesive conductive hydrogel patch is fabricated based on Fe3+-induced ionic coordination between dopamine-gelatin (GelDA) conjugates and dopamine-functionalized polypyrrole (DA-PPy), which form a homogeneous network.
31859471	4	43	theme	homogeneous	737:747	arg1	network					749:755	a homogeneous network	735:755	a homogeneous network	735:755	The self-adhesive conductive hydrogel patch is fabricated based on Fe3+-induced ionic coordination between dopamine-gelatin (GelDA) conjugates and dopamine-functionalized polypyrrole (DA-PPy), which form a homogeneous network.
31859471	6	44	dep	injecting	964:972	arg1	results					1099:1105	results	1099:1105	results in a more pronounced improvement of the cardiac function in terms of echocardiographical, histological, and angiogenic outcomes	1099:1233	Compared with a single-mode system, injecting the HA-CHO/HHA hydrogel intramyocardially followed by painting a conductive GelDA/DA-PPy hydrogel patch on the heart surface results in a more pronounced improvement of the cardiac function in terms of echocardiographical, histological, and angiogenic outcomes.
31859471	6	45	from	function	1155:1162	arg1	terms					1167:1171	terms	1167:1171	terms of echocardiographical, histological, and angiogenic outcomes	1167:1233	Compared with a single-mode system, injecting the HA-CHO/HHA hydrogel intramyocardially followed by painting a conductive GelDA/DA-PPy hydrogel patch on the heart surface results in a more pronounced improvement of the cardiac function in terms of echocardiographical, histological, and angiogenic outcomes.
31859471	1	46	theme	past	126:129	arg1	decade					131:136	the past decade	122:136	the past decade	122:136	Over the past decade, tissue-engineering strategies, mainly involving injectable hydrogels and epicardial biomaterial patches, have been pursued to treat myocardial infarction.
31859471	6	47	theme	hydrogel	1063:1070	arg1	patch					1072:1076	a conductive GelDA/DA-PPy hydrogel patch	1037:1076	a conductive GelDA/DA-PPy hydrogel patch on the heart surface	1037:1097	Compared with a single-mode system, injecting the HA-CHO/HHA hydrogel intramyocardially followed by painting a conductive GelDA/DA-PPy hydrogel patch on the heart surface results in a more pronounced improvement of the cardiac function in terms of echocardiographical, histological, and angiogenic outcomes.
31859471	6	48	theme	histological	1197:1208	arg1	outcomes					1226:1233	echocardiographical, histological, and angiogenic outcomes	1176:1233	echocardiographical, histological, and angiogenic outcomes	1176:1233	Compared with a single-mode system, injecting the HA-CHO/HHA hydrogel intramyocardially followed by painting a conductive GelDA/DA-PPy hydrogel patch on the heart surface results in a more pronounced improvement of the cardiac function in terms of echocardiographical, histological, and angiogenic outcomes.
31859471	3	49	theme	patch	472:476	arg1	coadministration					430:445	the coadministration	426:445	the coadministration of a conductive hydrogel patch	426:476	Here, a combined therapy approach is proposed, that is, the coadministration of a conductive hydrogel patch and injectable hydrogel to the infarcted myocardium.
31859471	3	49	theme	patch	472:476	arg1	hydrogel					493:500	injectable hydrogel	482:500	injectable hydrogel to the infarcted myocardium	482:528	Here, a combined therapy approach is proposed, that is, the coadministration of a conductive hydrogel patch and injectable hydrogel to the infarcted myocardium.
31859471	0	50	theme	Injectable	65:74	arg1	Hydrogel					76:83	an Injectable Hydrogel	62:83	an Injectable Hydrogel	62:83	Coadministration of an Adhesive Conductive Hydrogel Patch and an Injectable Hydrogel to Treat Myocardial Infarction.
31859471	5	51	theme	sodium	858:863	arg1	acid					876:879	oxidized sodium hyaluronic acid	849:879	oxidized sodium hyaluronic acid (HA-CHO)	849:888	The injectable and cleavable hydrogel is formed in situ via a Schiff base reaction between oxidized sodium hyaluronic acid (HA-CHO) and hydrazided hyaluronic acid (HHA).
31859471	5	51	theme	sodium	858:863	arg1	HA-CHO					882:887	HA-CHO	882:887	HA-CHO	882:887	The injectable and cleavable hydrogel is formed in situ via a Schiff base reaction between oxidized sodium hyaluronic acid (HA-CHO) and hydrazided hyaluronic acid (HHA).
31859471	3	52	theme	infarcted	509:517	arg1	myocardium					519:528	the infarcted myocardium	505:528	the infarcted myocardium	505:528	Here, a combined therapy approach is proposed, that is, the coadministration of a conductive hydrogel patch and injectable hydrogel to the infarcted myocardium.
31859471	6	53	theme	conductive	1039:1048	arg1	patch					1072:1076	a conductive GelDA/DA-PPy hydrogel patch	1037:1076	a conductive GelDA/DA-PPy hydrogel patch on the heart surface	1037:1097	Compared with a single-mode system, injecting the HA-CHO/HHA hydrogel intramyocardially followed by painting a conductive GelDA/DA-PPy hydrogel patch on the heart surface results in a more pronounced improvement of the cardiac function in terms of echocardiographical, histological, and angiogenic outcomes.
31859471	4	54	theme	ionic	611:615	arg1	coordination					617:628	Fe3+-induced ionic coordination	598:628	Fe3+-induced ionic coordination between dopamine-gelatin (GelDA) conjugates and dopamine-functionalized polypyrrole (DA-PPy), which form a homogeneous network	598:755	The self-adhesive conductive hydrogel patch is fabricated based on Fe3+-induced ionic coordination between dopamine-gelatin (GelDA) conjugates and dopamine-functionalized polypyrrole (DA-PPy), which form a homogeneous network.
31859471	5	55	theme	oxidized	849:856	arg1	acid					876:879	oxidized sodium hyaluronic acid	849:879	oxidized sodium hyaluronic acid (HA-CHO)	849:888	The injectable and cleavable hydrogel is formed in situ via a Schiff base reaction between oxidized sodium hyaluronic acid (HA-CHO) and hydrazided hyaluronic acid (HHA).
31859471	5	55	theme	oxidized	849:856	arg1	HA-CHO					882:887	HA-CHO	882:887	HA-CHO	882:887	The injectable and cleavable hydrogel is formed in situ via a Schiff base reaction between oxidized sodium hyaluronic acid (HA-CHO) and hydrazided hyaluronic acid (HHA).
31859471	1	56	theme	tissue-engineering	139:156	arg1	strategies					158:167	tissue-engineering strategies	139:167	tissue-engineering strategies	139:167	Over the past decade, tissue-engineering strategies, mainly involving injectable hydrogels and epicardial biomaterial patches, have been pursued to treat myocardial infarction.
31859471	4	57	theme	dopamine-gelatin	638:653	arg1	conjugates					663:672	dopamine-gelatin (GelDA) conjugates	638:672	dopamine-gelatin (GelDA) conjugates	638:672	The self-adhesive conductive hydrogel patch is fabricated based on Fe3+-induced ionic coordination between dopamine-gelatin (GelDA) conjugates and dopamine-functionalized polypyrrole (DA-PPy), which form a homogeneous network.
32987794	3	0	theme	SARS-CoV-2	546:555	arg1	structures					532:541	the glycosylation structures	514:541	the glycosylation structures of SARS-CoV-2	514:555	Comparison of the glycosylation structures of SARS-CoV-2 with the polysaccharide structures of pneumococcal vaccines yielded no obvious similarities.
32987794	6	1	theme	meningitis	1209:1218	arg1	vaccines					1220:1227	meningitis vaccines	1209:1227	meningitis vaccines	1209:1227	CRM197 is also present in Haemophilus influenzae type b (Hib) and meningitis vaccines.
32987794	0	2	theme	Pneumococcal	78:89	arg1	Vaccines					91:98	Pneumococcal Vaccines	78:98	Pneumococcal Vaccines	78:98	Possible Cross-Reactivity between SARS-CoV-2 Proteins, CRM197 and Proteins in Pneumococcal Vaccines May Protect Against Symptomatic SARS-CoV-2 Disease and Death.
32987794	0	3	from	Cross-Reactivity	9:24	arg1	Vaccines					91:98	Pneumococcal Vaccines	78:98	Pneumococcal Vaccines	78:98	Possible Cross-Reactivity between SARS-CoV-2 Proteins, CRM197 and Proteins in Pneumococcal Vaccines May Protect Against Symptomatic SARS-CoV-2 Disease and Death.
32987794	2	4	from	vaccines	368:375	arg1	particular					380:389	particular	380:389	particular	380:389	This paper explores the possibility that pneumococcal vaccines in particular, but perhaps other vaccines as well, contain antigens that might be cross-reactive with SARS-CoV-2 antigens.
32987794	6	5	located	present	1158:1164	arg1	Hib					1200:1202	Hib	1200:1202	Hib	1200:1202	CRM197 is also present in Haemophilus influenzae type b (Hib) and meningitis vaccines.
32987794	6	5	located	present	1158:1164	arg2	CRM197					1143:1148	CRM197	1143:1148	CRM197	1143:1148	CRM197 is also present in Haemophilus influenzae type b (Hib) and meningitis vaccines.
32987794	6	5	located	present	1158:1164	arg1	b					1197:1197	Haemophilus influenzae type b	1169:1197	Haemophilus influenzae type b (Hib)	1169:1203	CRM197 is also present in Haemophilus influenzae type b (Hib) and meningitis vaccines.
32987794	6	5	located	present	1158:1164	arg1	vaccines					1220:1227	meningitis vaccines	1209:1227	meningitis vaccines	1209:1227	CRM197 is also present in Haemophilus influenzae type b (Hib) and meningitis vaccines.
32987794	1	6	theme	SARS-CoV-2	282:291	arg1	infection					293:301	SARS-CoV-2 infection	282:301	SARS-CoV-2 infection	282:301	Various studies indicate that vaccination, especially with pneumococcal vaccines, protects against symptomatic cases of SARS-CoV-2 infection and death.
32987794	8	7	theme	SARS-CoV-2	1575:1584	arg1	disease					1586:1592	SARS-CoV-2 disease	1575:1592	SARS-CoV-2 disease	1575:1592	Notably, PspA and PspC are highly antigenic and new pneumococcal vaccines based on them are currently in human clinical trials so that their effectiveness against SARS-CoV-2 disease is easily testable.
32987794	5	8	theme	SARS-CoV-2	1059:1068	arg1	proteins					1070:1077	several SARS-CoV-2 proteins	1051:1077	several SARS-CoV-2 proteins including the spike protein, membrane protein and replicase 1a	1051:1140	All of these proteins have very high degrees of similarity, using very stringent criteria, with several SARS-CoV-2 proteins including the spike protein, membrane protein and replicase 1a.
32987794	5	8	theme	SARS-CoV-2	1059:1068	arg1	protein					1099:1105	the spike protein	1089:1105	the spike protein	1089:1105	All of these proteins have very high degrees of similarity, using very stringent criteria, with several SARS-CoV-2 proteins including the spike protein, membrane protein and replicase 1a.
32987794	5	8	theme	SARS-CoV-2	1059:1068	arg1	protein					1117:1123	membrane protein	1108:1123	membrane protein	1108:1123	All of these proteins have very high degrees of similarity, using very stringent criteria, with several SARS-CoV-2 proteins including the spike protein, membrane protein and replicase 1a.
32987794	5	8	theme	SARS-CoV-2	1059:1068	arg1	1a					1139:1140	replicase 1a	1129:1140	replicase 1a	1129:1140	All of these proteins have very high degrees of similarity, using very stringent criteria, with several SARS-CoV-2 proteins including the spike protein, membrane protein and replicase 1a.
32987794	7	9	theme	poliovirus	1391:1400	arg1	vaccines					1402:1409	poliovirus vaccines	1391:1409	poliovirus vaccines	1391:1409	Equivalent similarities were found at lower rates, or were completely absent, among the proteins in diphtheria, tetanus, pertussis, measles, mumps, rubella, and poliovirus vaccines.
32987794	3	10	theme	pneumococcal	595:606	arg1	vaccines					608:615	pneumococcal vaccines	595:615	pneumococcal vaccines	595:615	Comparison of the glycosylation structures of SARS-CoV-2 with the polysaccharide structures of pneumococcal vaccines yielded no obvious similarities.
32987794	1	11	theme	infection	293:301	arg1	cases					273:277	symptomatic cases	261:277	symptomatic cases of SARS-CoV-2 infection and death	261:311	Various studies indicate that vaccination, especially with pneumococcal vaccines, protects against symptomatic cases of SARS-CoV-2 infection and death.
32987794	5	12	theme	similarity	1003:1012	arg1	degrees					992:998	very high degrees	982:998	very high degrees of similarity	982:1012	All of these proteins have very high degrees of similarity, using very stringent criteria, with several SARS-CoV-2 proteins including the spike protein, membrane protein and replicase 1a.
32987794	4	13	dep	material	777:784	arg1	CRM197					786:791	CRM197	786:791	CRM197	786:791	However, while pneumococcal vaccines are primarily composed of capsular polysaccharides, some are conjugated to cross-reacting material CRM197, a modified diphtheria toxin, and all contain about three percent protein contaminants, including the pneumococcal surface proteins PsaA, PspA and probably PspC.
32987794	3	14	theme	vaccines	608:615	arg1	structures					581:590	the polysaccharide structures	562:590	the polysaccharide structures of pneumococcal vaccines	562:615	Comparison of the glycosylation structures of SARS-CoV-2 with the polysaccharide structures of pneumococcal vaccines yielded no obvious similarities.
32987794	3	15	theme	glycosylation	518:530	arg1	structures					532:541	the glycosylation structures	514:541	the glycosylation structures of SARS-CoV-2	514:555	Comparison of the glycosylation structures of SARS-CoV-2 with the polysaccharide structures of pneumococcal vaccines yielded no obvious similarities.
32987794	0	16	theme	Symptomatic	120:130	arg1	Disease					143:149	Symptomatic SARS-CoV-2 Disease	120:149	Symptomatic SARS-CoV-2 Disease	120:149	Possible Cross-Reactivity between SARS-CoV-2 Proteins, CRM197 and Proteins in Pneumococcal Vaccines May Protect Against Symptomatic SARS-CoV-2 Disease and Death.
32987794	4	17	theme	percent	851:857	arg1	contaminants					867:878	about three percent protein contaminants	839:878	about three percent protein contaminants	839:878	However, while pneumococcal vaccines are primarily composed of capsular polysaccharides, some are conjugated to cross-reacting material CRM197, a modified diphtheria toxin, and all contain about three percent protein contaminants, including the pneumococcal surface proteins PsaA, PspA and probably PspC.
32987794	4	17	theme	percent	851:857	arg1	proteins					916:923	the pneumococcal surface proteins	891:923	the pneumococcal surface proteins PsaA, PspA and probably PspC	891:952	However, while pneumococcal vaccines are primarily composed of capsular polysaccharides, some are conjugated to cross-reacting material CRM197, a modified diphtheria toxin, and all contain about three percent protein contaminants, including the pneumococcal surface proteins PsaA, PspA and probably PspC.
32987794	6	18	from	vaccines	1220:1227	arg1	present					1158:1164	present	1158:1164	present	1158:1164	CRM197 is also present in Haemophilus influenzae type b (Hib) and meningitis vaccines.
32987794	6	19	theme	type	1192:1195	arg1	Hib					1200:1202	Hib	1200:1202	Hib	1200:1202	CRM197 is also present in Haemophilus influenzae type b (Hib) and meningitis vaccines.
32987794	6	19	theme	type	1192:1195	arg1	b					1197:1197	Haemophilus influenzae type b	1169:1197	Haemophilus influenzae type b (Hib)	1169:1203	CRM197 is also present in Haemophilus influenzae type b (Hib) and meningitis vaccines.
32987794	2	20	theme	SARS-CoV-2	479:488	arg1	antigens					490:497	SARS-CoV-2 antigens	479:497	SARS-CoV-2 antigens	479:497	This paper explores the possibility that pneumococcal vaccines in particular, but perhaps other vaccines as well, contain antigens that might be cross-reactive with SARS-CoV-2 antigens.
32987794	3	21	theme	polysaccharide	566:579	arg1	structures					581:590	the polysaccharide structures	562:590	the polysaccharide structures of pneumococcal vaccines	562:615	Comparison of the glycosylation structures of SARS-CoV-2 with the polysaccharide structures of pneumococcal vaccines yielded no obvious similarities.
32987794	7	22	from	proteins	1318:1325	arg1	vaccines					1402:1409	poliovirus vaccines	1391:1409	poliovirus vaccines	1391:1409	Equivalent similarities were found at lower rates, or were completely absent, among the proteins in diphtheria, tetanus, pertussis, measles, mumps, rubella, and poliovirus vaccines.
32987794	7	22	from	proteins	1318:1325	arg1	rubella					1378:1384	rubella	1378:1384	rubella	1378:1384	Equivalent similarities were found at lower rates, or were completely absent, among the proteins in diphtheria, tetanus, pertussis, measles, mumps, rubella, and poliovirus vaccines.
32987794	7	22	from	proteins	1318:1325	arg1	mumps					1371:1375	diphtheria, tetanus, pertussis, measles, mumps	1330:1375	mumps	1371:1375	Equivalent similarities were found at lower rates, or were completely absent, among the proteins in diphtheria, tetanus, pertussis, measles, mumps, rubella, and poliovirus vaccines.
32987794	3	23	theme	obvious	628:634	arg1	similarities					636:647	no obvious similarities	625:647	no obvious similarities	625:647	Comparison of the glycosylation structures of SARS-CoV-2 with the polysaccharide structures of pneumococcal vaccines yielded no obvious similarities.
32987794	7	24	theme	Equivalent	1230:1239	arg1	similarities					1241:1252	Equivalent similarities	1230:1252	Equivalent similarities	1230:1252	Equivalent similarities were found at lower rates, or were completely absent, among the proteins in diphtheria, tetanus, pertussis, measles, mumps, rubella, and poliovirus vaccines.
32987794	0	25	theme	Possible	0:7	arg1	Cross-Reactivity					9:24	Possible Cross-Reactivity	0:24	Possible Cross-Reactivity between SARS-CoV-2 Proteins, CRM197 and Proteins in Pneumococcal Vaccines	0:98	Possible Cross-Reactivity between SARS-CoV-2 Proteins, CRM197 and Proteins in Pneumococcal Vaccines May Protect Against Symptomatic SARS-CoV-2 Disease and Death.
32987794	1	26	theme	death	307:311	arg1	cases					273:277	symptomatic cases	261:277	symptomatic cases of SARS-CoV-2 infection and death	261:311	Various studies indicate that vaccination, especially with pneumococcal vaccines, protects against symptomatic cases of SARS-CoV-2 infection and death.
32987794	4	27	theme	modified	796:803	arg1	material					777:784	material CRM197, a modified diphtheria toxin	777:820	material	777:784	However, while pneumococcal vaccines are primarily composed of capsular polysaccharides, some are conjugated to cross-reacting material CRM197, a modified diphtheria toxin, and all contain about three percent protein contaminants, including the pneumococcal surface proteins PsaA, PspA and probably PspC.
32987794	4	27	theme	modified	796:803	arg1	toxin					816:820	a modified diphtheria toxin	794:820	a modified diphtheria toxin	794:820	However, while pneumococcal vaccines are primarily composed of capsular polysaccharides, some are conjugated to cross-reacting material CRM197, a modified diphtheria toxin, and all contain about three percent protein contaminants, including the pneumococcal surface proteins PsaA, PspA and probably PspC.
32987794	5	28	theme	membrane	1108:1115	arg1	protein					1117:1123	membrane protein	1108:1123	membrane protein	1108:1123	All of these proteins have very high degrees of similarity, using very stringent criteria, with several SARS-CoV-2 proteins including the spike protein, membrane protein and replicase 1a.
32987794	0	29	theme	SARS-CoV-2	34:43	arg1	Proteins					45:52	SARS-CoV-2 Proteins	34:52	SARS-CoV-2 Proteins	34:52	Possible Cross-Reactivity between SARS-CoV-2 Proteins, CRM197 and Proteins in Pneumococcal Vaccines May Protect Against Symptomatic SARS-CoV-2 Disease and Death.
32987794	7	30	dep	mumps	1371:1375	arg1	measles					1362:1368	diphtheria, tetanus, pertussis, measles, mumps	1330:1375	measles	1362:1368	Equivalent similarities were found at lower rates, or were completely absent, among the proteins in diphtheria, tetanus, pertussis, measles, mumps, rubella, and poliovirus vaccines.
32987794	7	30	dep	mumps	1371:1375	arg1	pertussis					1351:1359	diphtheria, tetanus, pertussis, measles, mumps	1330:1375	pertussis	1351:1359	Equivalent similarities were found at lower rates, or were completely absent, among the proteins in diphtheria, tetanus, pertussis, measles, mumps, rubella, and poliovirus vaccines.
32987794	7	30	dep	mumps	1371:1375	arg1	tetanus					1342:1348	diphtheria, tetanus, pertussis, measles, mumps	1330:1375	tetanus	1342:1348	Equivalent similarities were found at lower rates, or were completely absent, among the proteins in diphtheria, tetanus, pertussis, measles, mumps, rubella, and poliovirus vaccines.
32987794	4	31	dep	proteins	916:923	arg1	PspA					931:934	PspA	931:934	PspA	931:934	However, while pneumococcal vaccines are primarily composed of capsular polysaccharides, some are conjugated to cross-reacting material CRM197, a modified diphtheria toxin, and all contain about three percent protein contaminants, including the pneumococcal surface proteins PsaA, PspA and probably PspC.
32987794	4	31	dep	proteins	916:923	arg1	PspC					949:952	PspC	949:952	PspC	949:952	However, while pneumococcal vaccines are primarily composed of capsular polysaccharides, some are conjugated to cross-reacting material CRM197, a modified diphtheria toxin, and all contain about three percent protein contaminants, including the pneumococcal surface proteins PsaA, PspA and probably PspC.
32987794	4	31	dep	proteins	916:923	arg1	PsaA					925:928	PsaA	925:928	PsaA	925:928	However, while pneumococcal vaccines are primarily composed of capsular polysaccharides, some are conjugated to cross-reacting material CRM197, a modified diphtheria toxin, and all contain about three percent protein contaminants, including the pneumococcal surface proteins PsaA, PspA and probably PspC.
32987794	4	31	dep	proteins	916:923	arg1	proteins					916:923	the pneumococcal surface proteins	891:923	the pneumococcal surface proteins PsaA, PspA and probably PspC	891:952	However, while pneumococcal vaccines are primarily composed of capsular polysaccharides, some are conjugated to cross-reacting material CRM197, a modified diphtheria toxin, and all contain about three percent protein contaminants, including the pneumococcal surface proteins PsaA, PspA and probably PspC.
32987794	0	32	theme	SARS-CoV-2	132:141	arg1	Disease					143:149	Symptomatic SARS-CoV-2 Disease	120:149	Symptomatic SARS-CoV-2 Disease	120:149	Possible Cross-Reactivity between SARS-CoV-2 Proteins, CRM197 and Proteins in Pneumococcal Vaccines May Protect Against Symptomatic SARS-CoV-2 Disease and Death.
32987794	8	33	theme	pneumococcal	1464:1475	arg1	vaccines					1477:1484	new pneumococcal vaccines	1460:1484	new pneumococcal vaccines based on them	1460:1498	Notably, PspA and PspC are highly antigenic and new pneumococcal vaccines based on them are currently in human clinical trials so that their effectiveness against SARS-CoV-2 disease is easily testable.
32987794	1	34	theme	pneumococcal	221:232	arg1	vaccines					234:241	pneumococcal vaccines	221:241	pneumococcal vaccines	221:241	Various studies indicate that vaccination, especially with pneumococcal vaccines, protects against symptomatic cases of SARS-CoV-2 infection and death.
32987794	5	35	theme	stringent	1026:1034	arg1	criteria					1036:1043	very stringent criteria	1021:1043	very stringent criteria	1021:1043	All of these proteins have very high degrees of similarity, using very stringent criteria, with several SARS-CoV-2 proteins including the spike protein, membrane protein and replicase 1a.
32987794	3	36	with	Comparison	500:509	arg1	structures					581:590	the polysaccharide structures	562:590	the polysaccharide structures of pneumococcal vaccines	562:615	Comparison of the glycosylation structures of SARS-CoV-2 with the polysaccharide structures of pneumococcal vaccines yielded no obvious similarities.
32987794	8	37	theme	new	1460:1462	arg1	vaccines					1477:1484	new pneumococcal vaccines	1460:1484	new pneumococcal vaccines based on them	1460:1498	Notably, PspA and PspC are highly antigenic and new pneumococcal vaccines based on them are currently in human clinical trials so that their effectiveness against SARS-CoV-2 disease is easily testable.
32987794	4	38	theme	pneumococcal	895:906	arg1	PspA					931:934	PspA	931:934	PspA	931:934	However, while pneumococcal vaccines are primarily composed of capsular polysaccharides, some are conjugated to cross-reacting material CRM197, a modified diphtheria toxin, and all contain about three percent protein contaminants, including the pneumococcal surface proteins PsaA, PspA and probably PspC.
32987794	4	38	theme	pneumococcal	895:906	arg1	PspC					949:952	PspC	949:952	PspC	949:952	However, while pneumococcal vaccines are primarily composed of capsular polysaccharides, some are conjugated to cross-reacting material CRM197, a modified diphtheria toxin, and all contain about three percent protein contaminants, including the pneumococcal surface proteins PsaA, PspA and probably PspC.
32987794	4	38	theme	pneumococcal	895:906	arg1	PsaA					925:928	PsaA	925:928	PsaA	925:928	However, while pneumococcal vaccines are primarily composed of capsular polysaccharides, some are conjugated to cross-reacting material CRM197, a modified diphtheria toxin, and all contain about three percent protein contaminants, including the pneumococcal surface proteins PsaA, PspA and probably PspC.
32987794	4	38	theme	pneumococcal	895:906	arg1	proteins					916:923	the pneumococcal surface proteins	891:923	the pneumococcal surface proteins PsaA, PspA and probably PspC	891:952	However, while pneumococcal vaccines are primarily composed of capsular polysaccharides, some are conjugated to cross-reacting material CRM197, a modified diphtheria toxin, and all contain about three percent protein contaminants, including the pneumococcal surface proteins PsaA, PspA and probably PspC.
32987794	5	39	theme	spike	1093:1097	arg1	protein					1099:1105	the spike protein	1089:1105	the spike protein	1089:1105	All of these proteins have very high degrees of similarity, using very stringent criteria, with several SARS-CoV-2 proteins including the spike protein, membrane protein and replicase 1a.
32987794	2	40	with	cross-reactive	459:472	arg1	antigens					490:497	SARS-CoV-2 antigens	479:497	SARS-CoV-2 antigens	479:497	This paper explores the possibility that pneumococcal vaccines in particular, but perhaps other vaccines as well, contain antigens that might be cross-reactive with SARS-CoV-2 antigens.
32987794	4	41	theme	capsular	713:720	arg1	polysaccharides					722:736	capsular polysaccharides	713:736	capsular polysaccharides	713:736	However, while pneumococcal vaccines are primarily composed of capsular polysaccharides, some are conjugated to cross-reacting material CRM197, a modified diphtheria toxin, and all contain about three percent protein contaminants, including the pneumococcal surface proteins PsaA, PspA and probably PspC.
32987794	6	42	attach	present	1158:1164	arg1	Hib					1200:1202	Hib	1200:1202	Hib	1200:1202	CRM197 is also present in Haemophilus influenzae type b (Hib) and meningitis vaccines.
32987794	6	42	attach	present	1158:1164	arg2	CRM197					1143:1148	CRM197	1143:1148	CRM197	1143:1148	CRM197 is also present in Haemophilus influenzae type b (Hib) and meningitis vaccines.
32987794	6	42	attach	present	1158:1164	arg1	b					1197:1197	Haemophilus influenzae type b	1169:1197	Haemophilus influenzae type b (Hib)	1169:1203	CRM197 is also present in Haemophilus influenzae type b (Hib) and meningitis vaccines.
32987794	6	42	attach	present	1158:1164	arg1	vaccines					1220:1227	meningitis vaccines	1209:1227	meningitis vaccines	1209:1227	CRM197 is also present in Haemophilus influenzae type b (Hib) and meningitis vaccines.
32987794	6	43	dep	Haemophilus	1169:1179	arg1	influenzae					1181:1190	influenzae	1181:1190	influenzae	1181:1190	CRM197 is also present in Haemophilus influenzae type b (Hib) and meningitis vaccines.
32987794	6	44	theme	Haemophilus	1169:1179	arg1	Hib					1200:1202	Hib	1200:1202	Hib	1200:1202	CRM197 is also present in Haemophilus influenzae type b (Hib) and meningitis vaccines.
32987794	6	44	theme	Haemophilus	1169:1179	arg1	b					1197:1197	Haemophilus influenzae type b	1169:1197	Haemophilus influenzae type b (Hib)	1169:1203	CRM197 is also present in Haemophilus influenzae type b (Hib) and meningitis vaccines.
32987794	3	45	theme	structures	532:541	arg1	Comparison					500:509	Comparison	500:509	Comparison of the glycosylation structures of SARS-CoV-2 with the polysaccharide structures of pneumococcal vaccines	500:615	Comparison of the glycosylation structures of SARS-CoV-2 with the polysaccharide structures of pneumococcal vaccines yielded no obvious similarities.
32987794	8	46	theme	clinical	1523:1530	arg1	trials					1532:1537	human clinical trials	1517:1537	human clinical trials	1517:1537	Notably, PspA and PspC are highly antigenic and new pneumococcal vaccines based on them are currently in human clinical trials so that their effectiveness against SARS-CoV-2 disease is easily testable.
32987794	4	47	contain	contain	831:837	arg2	proteins					916:923	the pneumococcal surface proteins	891:923	the pneumococcal surface proteins PsaA, PspA and probably PspC	891:952	However, while pneumococcal vaccines are primarily composed of capsular polysaccharides, some are conjugated to cross-reacting material CRM197, a modified diphtheria toxin, and all contain about three percent protein contaminants, including the pneumococcal surface proteins PsaA, PspA and probably PspC.
32987794	4	47	contain	contain	831:837	arg1	all					827:829	all	827:829	all	827:829	However, while pneumococcal vaccines are primarily composed of capsular polysaccharides, some are conjugated to cross-reacting material CRM197, a modified diphtheria toxin, and all contain about three percent protein contaminants, including the pneumococcal surface proteins PsaA, PspA and probably PspC.
32987794	4	47	contain	contain	831:837	arg2	contaminants					867:878	about three percent protein contaminants	839:878	about three percent protein contaminants	839:878	However, while pneumococcal vaccines are primarily composed of capsular polysaccharides, some are conjugated to cross-reacting material CRM197, a modified diphtheria toxin, and all contain about three percent protein contaminants, including the pneumococcal surface proteins PsaA, PspA and probably PspC.
32987794	2	48	contain	contain	428:434	arg2	antigens					436:443	antigens	436:443	antigens that might be cross-reactive with SARS-CoV-2 antigens	436:497	This paper explores the possibility that pneumococcal vaccines in particular, but perhaps other vaccines as well, contain antigens that might be cross-reactive with SARS-CoV-2 antigens.
32987794	2	48	contain	contain	428:434	arg1	vaccines					410:417	other vaccines	404:417	other vaccines as well	404:425	This paper explores the possibility that pneumococcal vaccines in particular, but perhaps other vaccines as well, contain antigens that might be cross-reactive with SARS-CoV-2 antigens.
32987794	2	48	contain	contain	428:434	arg1	vaccines					368:375	pneumococcal vaccines	355:375	pneumococcal vaccines in particular	355:389	This paper explores the possibility that pneumococcal vaccines in particular, but perhaps other vaccines as well, contain antigens that might be cross-reactive with SARS-CoV-2 antigens.
32987794	4	49	theme	surface	908:914	arg1	PspA					931:934	PspA	931:934	PspA	931:934	However, while pneumococcal vaccines are primarily composed of capsular polysaccharides, some are conjugated to cross-reacting material CRM197, a modified diphtheria toxin, and all contain about three percent protein contaminants, including the pneumococcal surface proteins PsaA, PspA and probably PspC.
32987794	4	49	theme	surface	908:914	arg1	PspC					949:952	PspC	949:952	PspC	949:952	However, while pneumococcal vaccines are primarily composed of capsular polysaccharides, some are conjugated to cross-reacting material CRM197, a modified diphtheria toxin, and all contain about three percent protein contaminants, including the pneumococcal surface proteins PsaA, PspA and probably PspC.
32987794	4	49	theme	surface	908:914	arg1	PsaA					925:928	PsaA	925:928	PsaA	925:928	However, while pneumococcal vaccines are primarily composed of capsular polysaccharides, some are conjugated to cross-reacting material CRM197, a modified diphtheria toxin, and all contain about three percent protein contaminants, including the pneumococcal surface proteins PsaA, PspA and probably PspC.
32987794	4	49	theme	surface	908:914	arg1	proteins					916:923	the pneumococcal surface proteins	891:923	the pneumococcal surface proteins PsaA, PspA and probably PspC	891:952	However, while pneumococcal vaccines are primarily composed of capsular polysaccharides, some are conjugated to cross-reacting material CRM197, a modified diphtheria toxin, and all contain about three percent protein contaminants, including the pneumococcal surface proteins PsaA, PspA and probably PspC.
32987794	7	50	located	found	1259:1263	arg2	similarities					1241:1252	Equivalent similarities	1230:1252	Equivalent similarities	1230:1252	Equivalent similarities were found at lower rates, or were completely absent, among the proteins in diphtheria, tetanus, pertussis, measles, mumps, rubella, and poliovirus vaccines.
32987794	7	50	located	found	1259:1263	arg1	rates					1274:1278	lower rates	1268:1278	lower rates	1268:1278	Equivalent similarities were found at lower rates, or were completely absent, among the proteins in diphtheria, tetanus, pertussis, measles, mumps, rubella, and poliovirus vaccines.
32987794	5	51	theme	high	987:990	arg1	degrees					992:998	very high degrees	982:998	very high degrees of similarity	982:1012	All of these proteins have very high degrees of similarity, using very stringent criteria, with several SARS-CoV-2 proteins including the spike protein, membrane protein and replicase 1a.
32987794	5	52	theme	several	1051:1057	arg1	proteins					1070:1077	several SARS-CoV-2 proteins	1051:1077	several SARS-CoV-2 proteins including the spike protein, membrane protein and replicase 1a	1051:1140	All of these proteins have very high degrees of similarity, using very stringent criteria, with several SARS-CoV-2 proteins including the spike protein, membrane protein and replicase 1a.
32987794	5	52	theme	several	1051:1057	arg1	protein					1099:1105	the spike protein	1089:1105	the spike protein	1089:1105	All of these proteins have very high degrees of similarity, using very stringent criteria, with several SARS-CoV-2 proteins including the spike protein, membrane protein and replicase 1a.
32987794	5	52	theme	several	1051:1057	arg1	protein					1117:1123	membrane protein	1108:1123	membrane protein	1108:1123	All of these proteins have very high degrees of similarity, using very stringent criteria, with several SARS-CoV-2 proteins including the spike protein, membrane protein and replicase 1a.
32987794	5	52	theme	several	1051:1057	arg1	1a					1139:1140	replicase 1a	1129:1140	replicase 1a	1129:1140	All of these proteins have very high degrees of similarity, using very stringent criteria, with several SARS-CoV-2 proteins including the spike protein, membrane protein and replicase 1a.
32987794	4	53	dep	conjugated	748:757	arg1	composed					701:708	composed	701:708	are primarily composed of capsular polysaccharides	687:736	However, while pneumococcal vaccines are primarily composed of capsular polysaccharides, some are conjugated to cross-reacting material CRM197, a modified diphtheria toxin, and all contain about three percent protein contaminants, including the pneumococcal surface proteins PsaA, PspA and probably PspC.
32987794	6	54	from	b	1197:1197	arg1	present					1158:1164	present	1158:1164	present	1158:1164	CRM197 is also present in Haemophilus influenzae type b (Hib) and meningitis vaccines.
32987794	4	55	theme	diphtheria	805:814	arg1	material					777:784	material CRM197, a modified diphtheria toxin	777:820	material	777:784	However, while pneumococcal vaccines are primarily composed of capsular polysaccharides, some are conjugated to cross-reacting material CRM197, a modified diphtheria toxin, and all contain about three percent protein contaminants, including the pneumococcal surface proteins PsaA, PspA and probably PspC.
32987794	4	55	theme	diphtheria	805:814	arg1	toxin					816:820	a modified diphtheria toxin	794:820	a modified diphtheria toxin	794:820	However, while pneumococcal vaccines are primarily composed of capsular polysaccharides, some are conjugated to cross-reacting material CRM197, a modified diphtheria toxin, and all contain about three percent protein contaminants, including the pneumococcal surface proteins PsaA, PspA and probably PspC.
32987794	1	56	with	vaccination	192:202	arg1	vaccines					234:241	pneumococcal vaccines	221:241	pneumococcal vaccines	221:241	Various studies indicate that vaccination, especially with pneumococcal vaccines, protects against symptomatic cases of SARS-CoV-2 infection and death.
32987794	4	57	theme	protein	859:865	arg1	contaminants					867:878	about three percent protein contaminants	839:878	about three percent protein contaminants	839:878	However, while pneumococcal vaccines are primarily composed of capsular polysaccharides, some are conjugated to cross-reacting material CRM197, a modified diphtheria toxin, and all contain about three percent protein contaminants, including the pneumococcal surface proteins PsaA, PspA and probably PspC.
32987794	4	57	theme	protein	859:865	arg1	proteins					916:923	the pneumococcal surface proteins	891:923	the pneumococcal surface proteins PsaA, PspA and probably PspC	891:952	However, while pneumococcal vaccines are primarily composed of capsular polysaccharides, some are conjugated to cross-reacting material CRM197, a modified diphtheria toxin, and all contain about three percent protein contaminants, including the pneumococcal surface proteins PsaA, PspA and probably PspC.
32987794	5	58	contain	have	977:980	arg1	proteins					968:975	these proteins	962:975	these proteins	962:975	All of these proteins have very high degrees of similarity, using very stringent criteria, with several SARS-CoV-2 proteins including the spike protein, membrane protein and replicase 1a.
32987794	5	58	contain	have	977:980	arg2	degrees					992:998	very high degrees	982:998	very high degrees of similarity	982:1012	All of these proteins have very high degrees of similarity, using very stringent criteria, with several SARS-CoV-2 proteins including the spike protein, membrane protein and replicase 1a.
32987794	5	58	contain	have	977:980	arg1	All					955:957	All	955:957	All	955:957	All of these proteins have very high degrees of similarity, using very stringent criteria, with several SARS-CoV-2 proteins including the spike protein, membrane protein and replicase 1a.
32987794	2	59	theme	pneumococcal	355:366	arg1	vaccines					368:375	pneumococcal vaccines	355:375	pneumococcal vaccines in particular	355:389	This paper explores the possibility that pneumococcal vaccines in particular, but perhaps other vaccines as well, contain antigens that might be cross-reactive with SARS-CoV-2 antigens.
32987794	4	60	theme	pneumococcal	665:676	arg1	vaccines					678:685	pneumococcal vaccines	665:685	pneumococcal vaccines	665:685	However, while pneumococcal vaccines are primarily composed of capsular polysaccharides, some are conjugated to cross-reacting material CRM197, a modified diphtheria toxin, and all contain about three percent protein contaminants, including the pneumococcal surface proteins PsaA, PspA and probably PspC.
32987794	5	61	theme	replicase	1129:1137	arg1	1a					1139:1140	replicase 1a	1129:1140	replicase 1a	1129:1140	All of these proteins have very high degrees of similarity, using very stringent criteria, with several SARS-CoV-2 proteins including the spike protein, membrane protein and replicase 1a.
32987794	2	62	theme	other	404:408	arg1	vaccines					410:417	other vaccines	404:417	other vaccines as well	404:425	This paper explores the possibility that pneumococcal vaccines in particular, but perhaps other vaccines as well, contain antigens that might be cross-reactive with SARS-CoV-2 antigens.
32987794	1	63	theme	symptomatic	261:271	arg1	cases					273:277	symptomatic cases	261:277	symptomatic cases of SARS-CoV-2 infection and death	261:311	Various studies indicate that vaccination, especially with pneumococcal vaccines, protects against symptomatic cases of SARS-CoV-2 infection and death.
32987794	6	64	from	present	1158:1164	arg1	Hib					1200:1202	Hib	1200:1202	Hib	1200:1202	CRM197 is also present in Haemophilus influenzae type b (Hib) and meningitis vaccines.
32987794	6	64	from	present	1158:1164	arg1	b					1197:1197	Haemophilus influenzae type b	1169:1197	Haemophilus influenzae type b (Hib)	1169:1203	CRM197 is also present in Haemophilus influenzae type b (Hib) and meningitis vaccines.
32987794	6	64	from	present	1158:1164	arg1	vaccines					1220:1227	meningitis vaccines	1209:1227	meningitis vaccines	1209:1227	CRM197 is also present in Haemophilus influenzae type b (Hib) and meningitis vaccines.
32987794	8	65	theme	human	1517:1521	arg1	trials					1532:1537	human clinical trials	1517:1537	human clinical trials	1517:1537	Notably, PspA and PspC are highly antigenic and new pneumococcal vaccines based on them are currently in human clinical trials so that their effectiveness against SARS-CoV-2 disease is easily testable.
32987794	7	66	theme	diphtheria	1330:1339	arg1	mumps					1371:1375	diphtheria, tetanus, pertussis, measles, mumps	1330:1375	mumps	1371:1375	Equivalent similarities were found at lower rates, or were completely absent, among the proteins in diphtheria, tetanus, pertussis, measles, mumps, rubella, and poliovirus vaccines.
32987794	1	67	theme	Various	162:168	arg1	studies					170:176	Various studies	162:176	Various studies	162:176	Various studies indicate that vaccination, especially with pneumococcal vaccines, protects against symptomatic cases of SARS-CoV-2 infection and death.
32987794	7	68	theme	lower	1268:1272	arg1	rates					1274:1278	lower rates	1268:1278	lower rates	1268:1278	Equivalent similarities were found at lower rates, or were completely absent, among the proteins in diphtheria, tetanus, pertussis, measles, mumps, rubella, and poliovirus vaccines.
33108417	0	0	theme	adipose	82:88	arg1	therapy					100:106	adipose stem cell therapy	82:106	adipose stem cell therapy	82:106	In vitro study of SDF-1α-loaded injectable and thermally responsive hydrogels for adipose stem cell therapy by SDF-1/CXCR4 axis.
33108417	3	1	theme	sustained	690:698	arg1	release					700:706	local and sustained release	680:706	local and sustained release of SDF-1α for ASC recruitment	680:736	Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels with different concentrations of hyaluronic acid (HA) were designed and fabricated to achieve local and sustained release of SDF-1α for ASC recruitment.
33108417	4	2	theme	different	851:859	arg1	compositions					861:872	different compositions	851:872	different compositions	851:872	Herein, the material structures, physical properties, gelation temperature, and gelation time of hydrogels with different compositions were determined.
33108417	7	3	theme	sustained	1231:1239	arg1	release					1241:1247	sustained release	1231:1247	sustained release of SDF-1α	1231:1257	In addition, the in vitro release profiles showed that the hydrogels achieved sustained release of SDF-1α over 7 days and enhanced ASC migration.
33108417	8	4	theme	injectable	1508:1517	arg1	hydrogels					1519:1527	the injectable hydrogels	1504:1527	the injectable hydrogels	1504:1527	The results revealed that the hydrogels with HA enhanced the sustained release effect compared with the hydrogel without HA, indicating that the HA content regulated the physical and release properties of the injectable hydrogels.
33108417	9	5	theme	CS/βGP/HA	1573:1581	arg1	hydrogels					1583:1591	thermoresponsive and injectable CS/βGP/HA hydrogels	1541:1591	thermoresponsive and injectable CS/βGP/HA hydrogels	1541:1591	Therefore, thermoresponsive and injectable CS/βGP/HA hydrogels may provide an alternative for treating ischemic diseases via SDF-1/CXCR4 axis for ASC recruitment and retention.
33108417	2	6	theme	therapeutic	452:462	arg1	angiogenesis					464:475	therapeutic angiogenesis	452:475	therapeutic angiogenesis	452:475	The aim of this study was to develop injectable hydrogel systems for the local release of stromal cell-derived factor-1α (SDF-1α) to recruit adipose stem cells (ASCs) that express CXCR4 to achieve stem cell therapy and therapeutic angiogenesis.
33108417	3	7	theme	injectable	499:508	arg1	CS					520:521	CS	520:521	CS	520:521	Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels with different concentrations of hyaluronic acid (HA) were designed and fabricated to achieve local and sustained release of SDF-1α for ASC recruitment.
33108417	3	7	theme	injectable	499:508	arg1	chitosan					510:517	injectable chitosan	499:517	injectable chitosan (CS)	499:522	Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels with different concentrations of hyaluronic acid (HA) were designed and fabricated to achieve local and sustained release of SDF-1α for ASC recruitment.
33108417	8	8	theme	sustained	1360:1368	arg1	effect					1378:1383	the sustained release effect	1356:1383	the sustained release effect	1356:1383	The results revealed that the hydrogels with HA enhanced the sustained release effect compared with the hydrogel without HA, indicating that the HA content regulated the physical and release properties of the injectable hydrogels.
33108417	9	9	theme	SDF-1/CXCR4	1655:1665	arg1	axis					1667:1670	SDF-1/CXCR4 axis	1655:1670	SDF-1/CXCR4 axis	1655:1670	Therefore, thermoresponsive and injectable CS/βGP/HA hydrogels may provide an alternative for treating ischemic diseases via SDF-1/CXCR4 axis for ASC recruitment and retention.
33108417	0	10	theme	cell	95:98	arg1	therapy					100:106	adipose stem cell therapy	82:106	adipose stem cell therapy	82:106	In vitro study of SDF-1α-loaded injectable and thermally responsive hydrogels for adipose stem cell therapy by SDF-1/CXCR4 axis.
33108417	2	11	link	cell-derived	331:342	arg1	SDF-1α					355:360	SDF-1α	355:360	SDF-1α	355:360	The aim of this study was to develop injectable hydrogel systems for the local release of stromal cell-derived factor-1α (SDF-1α) to recruit adipose stem cells (ASCs) that express CXCR4 to achieve stem cell therapy and therapeutic angiogenesis.
33108417	2	11	link	cell-derived	331:342	arg1	factor-1α					344:352	stromal cell-derived factor-1α	323:352	stromal cell-derived factor-1α (SDF-1α)	323:361	The aim of this study was to develop injectable hydrogel systems for the local release of stromal cell-derived factor-1α (SDF-1α) to recruit adipose stem cells (ASCs) that express CXCR4 to achieve stem cell therapy and therapeutic angiogenesis.
33108417	5	12	from	incorporation	895:907	arg1	hydrogels					932:940	CS-based hydrogels	923:940	CS-based hydrogels	923:940	The incorporation of 0.9% HA in CS-based hydrogels not only enhanced the gelation time but also increased the strength of the hydrogels.
33108417	3	13	theme	/β-glycerophosphate	523:541	arg1	hydrogels					576:584	Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels	478:584	Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels with different concentrations of hyaluronic acid (HA)	478:638	Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels with different concentrations of hyaluronic acid (HA) were designed and fabricated to achieve local and sustained release of SDF-1α for ASC recruitment.
33108417	6	14	theme	good	1130:1133	arg1	biocompatibility					1135:1150	good biocompatibility	1130:1150	good biocompatibility	1130:1150	In addition, the results revealed that the thermoresponsive and injectable CS/βGP/HA hydrogels showed good biocompatibility.
33108417	0	15	theme	stem	90:93	arg1	therapy					100:106	adipose stem cell therapy	82:106	adipose stem cell therapy	82:106	In vitro study of SDF-1α-loaded injectable and thermally responsive hydrogels for adipose stem cell therapy by SDF-1/CXCR4 axis.
33108417	3	16	theme	SDF-1α	711:716	arg1	release					700:706	local and sustained release	680:706	local and sustained release of SDF-1α for ASC recruitment	680:736	Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels with different concentrations of hyaluronic acid (HA) were designed and fabricated to achieve local and sustained release of SDF-1α for ASC recruitment.
33108417	3	17	theme	disodium	543:550	arg1	hydrogels					576:584	Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels	478:584	Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels with different concentrations of hyaluronic acid (HA)	478:638	Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels with different concentrations of hyaluronic acid (HA) were designed and fabricated to achieve local and sustained release of SDF-1α for ASC recruitment.
33108417	2	18	theme	hydrogel	281:288	arg1	systems					290:296	injectable hydrogel systems	270:296	injectable hydrogel systems for the local release of stromal cell-derived factor-1α (SDF-1α)	270:361	The aim of this study was to develop injectable hydrogel systems for the local release of stromal cell-derived factor-1α (SDF-1α) to recruit adipose stem cells (ASCs) that express CXCR4 to achieve stem cell therapy and therapeutic angiogenesis.
33108417	5	19	theme	HA	917:918	arg1	incorporation					895:907	The incorporation	891:907	The incorporation of 0.9% HA in CS-based hydrogels	891:940	The incorporation of 0.9% HA in CS-based hydrogels not only enhanced the gelation time but also increased the strength of the hydrogels.
33108417	5	20	theme	CS-based	923:930	arg1	hydrogels					932:940	CS-based hydrogels	923:940	CS-based hydrogels	923:940	The incorporation of 0.9% HA in CS-based hydrogels not only enhanced the gelation time but also increased the strength of the hydrogels.
33108417	2	21	theme	injectable	270:279	arg1	systems					290:296	injectable hydrogel systems	270:296	injectable hydrogel systems for the local release of stromal cell-derived factor-1α (SDF-1α)	270:361	The aim of this study was to develop injectable hydrogel systems for the local release of stromal cell-derived factor-1α (SDF-1α) to recruit adipose stem cells (ASCs) that express CXCR4 to achieve stem cell therapy and therapeutic angiogenesis.
33108417	2	22	theme	adipose	374:380	arg1	ASCs					394:397	ASCs	394:397	ASCs	394:397	The aim of this study was to develop injectable hydrogel systems for the local release of stromal cell-derived factor-1α (SDF-1α) to recruit adipose stem cells (ASCs) that express CXCR4 to achieve stem cell therapy and therapeutic angiogenesis.
33108417	2	22	theme	adipose	374:380	arg1	cells					387:391	adipose stem cells	374:391	adipose stem cells (ASCs) that express CXCR4 to achieve stem cell therapy and therapeutic angiogenesis	374:475	The aim of this study was to develop injectable hydrogel systems for the local release of stromal cell-derived factor-1α (SDF-1α) to recruit adipose stem cells (ASCs) that express CXCR4 to achieve stem cell therapy and therapeutic angiogenesis.
33108417	7	23	theme	release	1179:1185	arg1	profiles					1187:1194	the in vitro release profiles	1166:1194	the in vitro release profiles	1166:1194	In addition, the in vitro release profiles showed that the hydrogels achieved sustained release of SDF-1α over 7 days and enhanced ASC migration.
33108417	4	24	theme	gelation	819:826	arg1	time					828:831	gelation time	819:831	gelation time	819:831	Herein, the material structures, physical properties, gelation temperature, and gelation time of hydrogels with different compositions were determined.
33108417	4	25	theme	material	751:758	arg1	structures					760:769	the material structures	747:769	the material structures	747:769	Herein, the material structures, physical properties, gelation temperature, and gelation time of hydrogels with different compositions were determined.
33108417	2	26	theme	stem	382:385	arg1	ASCs					394:397	ASCs	394:397	ASCs	394:397	The aim of this study was to develop injectable hydrogel systems for the local release of stromal cell-derived factor-1α (SDF-1α) to recruit adipose stem cells (ASCs) that express CXCR4 to achieve stem cell therapy and therapeutic angiogenesis.
33108417	2	26	theme	stem	382:385	arg1	cells					387:391	adipose stem cells	374:391	adipose stem cells (ASCs) that express CXCR4 to achieve stem cell therapy and therapeutic angiogenesis	374:475	The aim of this study was to develop injectable hydrogel systems for the local release of stromal cell-derived factor-1α (SDF-1α) to recruit adipose stem cells (ASCs) that express CXCR4 to achieve stem cell therapy and therapeutic angiogenesis.
33108417	8	27	theme	hydrogels	1519:1527	arg1	properties					1490:1499	the physical and release properties	1465:1499	properties	1490:1499	The results revealed that the hydrogels with HA enhanced the sustained release effect compared with the hydrogel without HA, indicating that the HA content regulated the physical and release properties of the injectable hydrogels.
33108417	4	28	theme	hydrogels	836:844	arg1	properties					781:790	physical properties	772:790	physical properties	772:790	Herein, the material structures, physical properties, gelation temperature, and gelation time of hydrogels with different compositions were determined.
33108417	4	28	theme	hydrogels	836:844	arg1	temperature					802:812	gelation temperature	793:812	gelation temperature	793:812	Herein, the material structures, physical properties, gelation temperature, and gelation time of hydrogels with different compositions were determined.
33108417	4	28	theme	hydrogels	836:844	arg1	time					828:831	gelation time	819:831	gelation time	819:831	Herein, the material structures, physical properties, gelation temperature, and gelation time of hydrogels with different compositions were determined.
33108417	4	28	theme	hydrogels	836:844	arg1	structures					760:769	the material structures	747:769	the material structures	747:769	Herein, the material structures, physical properties, gelation temperature, and gelation time of hydrogels with different compositions were determined.
33108417	9	29	theme	injectable	1562:1571	arg1	hydrogels					1583:1591	thermoresponsive and injectable CS/βGP/HA hydrogels	1541:1591	thermoresponsive and injectable CS/βGP/HA hydrogels	1541:1591	Therefore, thermoresponsive and injectable CS/βGP/HA hydrogels may provide an alternative for treating ischemic diseases via SDF-1/CXCR4 axis for ASC recruitment and retention.
33108417	1	30	theme	therapeutic	180:190	arg1	strategy					192:199	a promising therapeutic strategy	168:199	a promising therapeutic strategy for treating ischemic diseases	168:230	Stem cell-based approaches have become a promising therapeutic strategy for treating ischemic diseases.
33108417	0	31	theme	In	0:1	arg1	study					9:13	In vitro study	0:13	In vitro study of SDF-1α-loaded injectable and thermally responsive hydrogels for adipose stem cell therapy by SDF-1/CXCR4 axis	0:126	In vitro study of SDF-1α-loaded injectable and thermally responsive hydrogels for adipose stem cell therapy by SDF-1/CXCR4 axis.
33108417	9	32	theme	ischemic	1633:1640	arg1	diseases					1642:1649	ischemic diseases	1633:1649	ischemic diseases	1633:1649	Therefore, thermoresponsive and injectable CS/βGP/HA hydrogels may provide an alternative for treating ischemic diseases via SDF-1/CXCR4 axis for ASC recruitment and retention.
33108417	2	33	theme	local	306:310	arg1	release					312:318	the local release	302:318	the local release of stromal cell-derived factor-1α (SDF-1α)	302:361	The aim of this study was to develop injectable hydrogel systems for the local release of stromal cell-derived factor-1α (SDF-1α) to recruit adipose stem cells (ASCs) that express CXCR4 to achieve stem cell therapy and therapeutic angiogenesis.
33108417	3	34	theme	local	680:684	arg1	release					700:706	local and sustained release	680:706	local and sustained release of SDF-1α for ASC recruitment	680:736	Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels with different concentrations of hyaluronic acid (HA) were designed and fabricated to achieve local and sustained release of SDF-1α for ASC recruitment.
33108417	3	35	theme	salt	552:555	arg1	hydrogels					576:584	Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels	478:584	Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels with different concentrations of hyaluronic acid (HA)	478:638	Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels with different concentrations of hyaluronic acid (HA) were designed and fabricated to achieve local and sustained release of SDF-1α for ASC recruitment.
33108417	3	36	theme	βGP	571:573	arg1	hydrogels					576:584	Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels	478:584	Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels with different concentrations of hyaluronic acid (HA)	478:638	Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels with different concentrations of hyaluronic acid (HA) were designed and fabricated to achieve local and sustained release of SDF-1α for ASC recruitment.
33108417	3	37	theme	ASC	722:724	arg1	recruitment					726:736	ASC recruitment	722:736	ASC recruitment	722:736	Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels with different concentrations of hyaluronic acid (HA) were designed and fabricated to achieve local and sustained release of SDF-1α for ASC recruitment.
33108417	0	38	theme	SDF-1/CXCR4	111:121	arg1	axis					123:126	SDF-1/CXCR4 axis	111:126	SDF-1/CXCR4 axis	111:126	In vitro study of SDF-1α-loaded injectable and thermally responsive hydrogels for adipose stem cell therapy by SDF-1/CXCR4 axis.
33108417	3	39	theme	pentahydrate	557:568	arg1	hydrogels					576:584	Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels	478:584	Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels with different concentrations of hyaluronic acid (HA)	478:638	Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels with different concentrations of hyaluronic acid (HA) were designed and fabricated to achieve local and sustained release of SDF-1α for ASC recruitment.
33108417	0	40	theme	injectable	32:41	arg1	hydrogels					68:76	SDF-1α-loaded injectable and thermally responsive hydrogels	18:76	SDF-1α-loaded injectable and thermally responsive hydrogels for adipose stem cell therapy	18:106	In vitro study of SDF-1α-loaded injectable and thermally responsive hydrogels for adipose stem cell therapy by SDF-1/CXCR4 axis.
33108417	3	41	theme	Thermoresponsive	478:493	arg1	hydrogels					576:584	Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels	478:584	Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels with different concentrations of hyaluronic acid (HA)	478:638	Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels with different concentrations of hyaluronic acid (HA) were designed and fabricated to achieve local and sustained release of SDF-1α for ASC recruitment.
33108417	7	42	theme	in	1170:1171	arg1	profiles					1187:1194	the in vitro release profiles	1166:1194	the in vitro release profiles	1166:1194	In addition, the in vitro release profiles showed that the hydrogels achieved sustained release of SDF-1α over 7 days and enhanced ASC migration.
33108417	1	43	theme	ischemic	214:221	arg1	diseases					223:230	ischemic diseases	214:230	ischemic diseases	214:230	Stem cell-based approaches have become a promising therapeutic strategy for treating ischemic diseases.
33108417	0	44	theme	SDF-1α-loaded	18:30	arg1	hydrogels					68:76	SDF-1α-loaded injectable and thermally responsive hydrogels	18:76	SDF-1α-loaded injectable and thermally responsive hydrogels for adipose stem cell therapy	18:106	In vitro study of SDF-1α-loaded injectable and thermally responsive hydrogels for adipose stem cell therapy by SDF-1/CXCR4 axis.
33108417	8	45	theme	HA	1444:1445	arg1	content					1447:1453	the HA content	1440:1453	the HA content	1440:1453	The results revealed that the hydrogels with HA enhanced the sustained release effect compared with the hydrogel without HA, indicating that the HA content regulated the physical and release properties of the injectable hydrogels.
33108417	3	46	theme	different	591:599	arg1	concentrations					601:614	different concentrations	591:614	different concentrations of hyaluronic acid (HA)	591:638	Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels with different concentrations of hyaluronic acid (HA) were designed and fabricated to achieve local and sustained release of SDF-1α for ASC recruitment.
33108417	7	47	theme	ASC	1284:1286	arg1	migration					1288:1296	ASC migration	1284:1296	ASC migration	1284:1296	In addition, the in vitro release profiles showed that the hydrogels achieved sustained release of SDF-1α over 7 days and enhanced ASC migration.
33108417	2	48	theme	cell	435:438	arg1	therapy					440:446	stem cell therapy	430:446	stem cell therapy	430:446	The aim of this study was to develop injectable hydrogel systems for the local release of stromal cell-derived factor-1α (SDF-1α) to recruit adipose stem cells (ASCs) that express CXCR4 to achieve stem cell therapy and therapeutic angiogenesis.
33108417	6	49	theme	CS/βGP/HA	1103:1111	arg1	hydrogels					1113:1121	the thermoresponsive and injectable CS/βGP/HA hydrogels	1067:1121	the thermoresponsive and injectable CS/βGP/HA hydrogels	1067:1121	In addition, the results revealed that the thermoresponsive and injectable CS/βGP/HA hydrogels showed good biocompatibility.
33108417	2	50	theme	factor-1α	344:352	arg1	release					312:318	the local release	302:318	the local release of stromal cell-derived factor-1α (SDF-1α)	302:361	The aim of this study was to develop injectable hydrogel systems for the local release of stromal cell-derived factor-1α (SDF-1α) to recruit adipose stem cells (ASCs) that express CXCR4 to achieve stem cell therapy and therapeutic angiogenesis.
33108417	2	51	theme	stem	430:433	arg1	therapy					440:446	stem cell therapy	430:446	stem cell therapy	430:446	The aim of this study was to develop injectable hydrogel systems for the local release of stromal cell-derived factor-1α (SDF-1α) to recruit adipose stem cells (ASCs) that express CXCR4 to achieve stem cell therapy and therapeutic angiogenesis.
33108417	5	52	theme	hydrogels	1017:1025	arg1	strength					1001:1008	the strength	997:1008	the strength of the hydrogels	997:1025	The incorporation of 0.9% HA in CS-based hydrogels not only enhanced the gelation time but also increased the strength of the hydrogels.
33108417	9	53	theme	ASC	1676:1678	arg1	recruitment					1680:1690	ASC recruitment	1676:1690	ASC recruitment	1676:1690	Therefore, thermoresponsive and injectable CS/βGP/HA hydrogels may provide an alternative for treating ischemic diseases via SDF-1/CXCR4 axis for ASC recruitment and retention.
33108417	2	54	theme	cell-derived	331:342	arg1	SDF-1α					355:360	SDF-1α	355:360	SDF-1α	355:360	The aim of this study was to develop injectable hydrogel systems for the local release of stromal cell-derived factor-1α (SDF-1α) to recruit adipose stem cells (ASCs) that express CXCR4 to achieve stem cell therapy and therapeutic angiogenesis.
33108417	2	54	theme	cell-derived	331:342	arg1	factor-1α					344:352	stromal cell-derived factor-1α	323:352	stromal cell-derived factor-1α (SDF-1α)	323:361	The aim of this study was to develop injectable hydrogel systems for the local release of stromal cell-derived factor-1α (SDF-1α) to recruit adipose stem cells (ASCs) that express CXCR4 to achieve stem cell therapy and therapeutic angiogenesis.
33108417	7	55	theme	SDF-1α	1252:1257	arg1	release					1241:1247	sustained release	1231:1247	sustained release of SDF-1α	1231:1257	In addition, the in vitro release profiles showed that the hydrogels achieved sustained release of SDF-1α over 7 days and enhanced ASC migration.
33108417	7	56	dep	in	1170:1171	arg1	vitro					1173:1177	vitro	1173:1177	vitro	1173:1177	In addition, the in vitro release profiles showed that the hydrogels achieved sustained release of SDF-1α over 7 days and enhanced ASC migration.
33108417	4	57	theme	physical	772:779	arg1	properties					781:790	physical properties	772:790	physical properties	772:790	Herein, the material structures, physical properties, gelation temperature, and gelation time of hydrogels with different compositions were determined.
33108417	3	58	theme	hyaluronic	619:628	arg1	HA					636:637	HA	636:637	HA	636:637	Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels with different concentrations of hyaluronic acid (HA) were designed and fabricated to achieve local and sustained release of SDF-1α for ASC recruitment.
33108417	3	58	theme	hyaluronic	619:628	arg1	acid					630:633	hyaluronic acid	619:633	hyaluronic acid (HA)	619:638	Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels with different concentrations of hyaluronic acid (HA) were designed and fabricated to achieve local and sustained release of SDF-1α for ASC recruitment.
33108417	4	59	theme	gelation	793:800	arg1	temperature					802:812	gelation temperature	793:812	gelation temperature	793:812	Herein, the material structures, physical properties, gelation temperature, and gelation time of hydrogels with different compositions were determined.
33108417	2	60	theme	stromal	323:329	arg1	SDF-1α					355:360	SDF-1α	355:360	SDF-1α	355:360	The aim of this study was to develop injectable hydrogel systems for the local release of stromal cell-derived factor-1α (SDF-1α) to recruit adipose stem cells (ASCs) that express CXCR4 to achieve stem cell therapy and therapeutic angiogenesis.
33108417	2	60	theme	stromal	323:329	arg1	factor-1α					344:352	stromal cell-derived factor-1α	323:352	stromal cell-derived factor-1α (SDF-1α)	323:361	The aim of this study was to develop injectable hydrogel systems for the local release of stromal cell-derived factor-1α (SDF-1α) to recruit adipose stem cells (ASCs) that express CXCR4 to achieve stem cell therapy and therapeutic angiogenesis.
33108417	6	61	theme	injectable	1092:1101	arg1	hydrogels					1113:1121	the thermoresponsive and injectable CS/βGP/HA hydrogels	1067:1121	the thermoresponsive and injectable CS/βGP/HA hydrogels	1067:1121	In addition, the results revealed that the thermoresponsive and injectable CS/βGP/HA hydrogels showed good biocompatibility.
33108417	5	62	theme	gelation	964:971	arg1	time					973:976	the gelation time	960:976	the gelation time	960:976	The incorporation of 0.9% HA in CS-based hydrogels not only enhanced the gelation time but also increased the strength of the hydrogels.
33108417	8	63	theme	physical	1469:1476	arg1	properties					1490:1499	the physical and release properties	1465:1499	properties	1490:1499	The results revealed that the hydrogels with HA enhanced the sustained release effect compared with the hydrogel without HA, indicating that the HA content regulated the physical and release properties of the injectable hydrogels.
33108417	3	64	theme	acid	630:633	arg1	concentrations					601:614	different concentrations	591:614	different concentrations of hyaluronic acid (HA)	591:638	Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels with different concentrations of hyaluronic acid (HA) were designed and fabricated to achieve local and sustained release of SDF-1α for ASC recruitment.
33108417	5	65	theme	0.9	912:914	arg1	%					915:915	%	915:915	%	915:915	The incorporation of 0.9% HA in CS-based hydrogels not only enhanced the gelation time but also increased the strength of the hydrogels.
33108417	5	66	theme	%	915:915	arg1	HA					917:918	0.9% HA	912:918	0.9% HA	912:918	The incorporation of 0.9% HA in CS-based hydrogels not only enhanced the gelation time but also increased the strength of the hydrogels.
33108417	0	67	theme	hydrogels	68:76	arg1	study					9:13	In vitro study	0:13	In vitro study of SDF-1α-loaded injectable and thermally responsive hydrogels for adipose stem cell therapy by SDF-1/CXCR4 axis	0:126	In vitro study of SDF-1α-loaded injectable and thermally responsive hydrogels for adipose stem cell therapy by SDF-1/CXCR4 axis.
33108417	8	68	theme	release	1370:1376	arg1	effect					1378:1383	the sustained release effect	1356:1383	the sustained release effect	1356:1383	The results revealed that the hydrogels with HA enhanced the sustained release effect compared with the hydrogel without HA, indicating that the HA content regulated the physical and release properties of the injectable hydrogels.
33108417	2	69	theme	study	249:253	arg1	aim					237:239	The aim	233:239	The aim of this study	233:253	The aim of this study was to develop injectable hydrogel systems for the local release of stromal cell-derived factor-1α (SDF-1α) to recruit adipose stem cells (ASCs) that express CXCR4 to achieve stem cell therapy and therapeutic angiogenesis.
33108417	1	70	theme	cell-based	134:143	arg1	approaches					145:154	Stem cell-based approaches	129:154	Stem cell-based approaches	129:154	Stem cell-based approaches have become a promising therapeutic strategy for treating ischemic diseases.
33108417	1	71	theme	promising	170:178	arg1	strategy					192:199	a promising therapeutic strategy	168:199	a promising therapeutic strategy for treating ischemic diseases	168:230	Stem cell-based approaches have become a promising therapeutic strategy for treating ischemic diseases.
33108417	8	72	with	hydrogels	1329:1337	arg1	HA					1344:1345	HA	1344:1345	HA	1344:1345	The results revealed that the hydrogels with HA enhanced the sustained release effect compared with the hydrogel without HA, indicating that the HA content regulated the physical and release properties of the injectable hydrogels.
33108417	0	73	theme	responsive	57:66	arg1	hydrogels					68:76	SDF-1α-loaded injectable and thermally responsive hydrogels	18:76	SDF-1α-loaded injectable and thermally responsive hydrogels for adipose stem cell therapy	18:106	In vitro study of SDF-1α-loaded injectable and thermally responsive hydrogels for adipose stem cell therapy by SDF-1/CXCR4 axis.
33108417	8	74	theme	release	1482:1488	arg1	properties					1490:1499	the physical and release properties	1465:1499	properties	1490:1499	The results revealed that the hydrogels with HA enhanced the sustained release effect compared with the hydrogel without HA, indicating that the HA content regulated the physical and release properties of the injectable hydrogels.
33108417	0	75	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro study of SDF-1α-loaded injectable and thermally responsive hydrogels for adipose stem cell therapy by SDF-1/CXCR4 axis.
33108417	9	76	theme	thermoresponsive	1541:1556	arg1	hydrogels					1583:1591	thermoresponsive and injectable CS/βGP/HA hydrogels	1541:1591	thermoresponsive and injectable CS/βGP/HA hydrogels	1541:1591	Therefore, thermoresponsive and injectable CS/βGP/HA hydrogels may provide an alternative for treating ischemic diseases via SDF-1/CXCR4 axis for ASC recruitment and retention.
33108417	6	77	theme	thermoresponsive	1071:1086	arg1	hydrogels					1113:1121	the thermoresponsive and injectable CS/βGP/HA hydrogels	1067:1121	the thermoresponsive and injectable CS/βGP/HA hydrogels	1067:1121	In addition, the results revealed that the thermoresponsive and injectable CS/βGP/HA hydrogels showed good biocompatibility.
33108417	4	78	with	hydrogels	836:844	arg1	compositions					861:872	different compositions	851:872	different compositions	851:872	Herein, the material structures, physical properties, gelation temperature, and gelation time of hydrogels with different compositions were determined.
33108417	3	79	with	hydrogels	576:584	arg1	concentrations					601:614	different concentrations	591:614	different concentrations of hyaluronic acid (HA)	591:638	Thermoresponsive and injectable chitosan (CS)/β-glycerophosphate disodium salt pentahydrate (βGP) hydrogels with different concentrations of hyaluronic acid (HA) were designed and fabricated to achieve local and sustained release of SDF-1α for ASC recruitment.
34445582	4	0	theme	precipitation	506:518	arg1	solution					520:527	ExoQuick-TC exosome precipitation solution	486:527	ExoQuick-TC exosome precipitation solution	486:527	ExoQuick-TC exosome precipitation solution was used to purify exosomes from human ADSC culture media in the presence or absence of 1 µg/mL LPS treatment for 24 h.
34445582	6	1	theme	formation	993:1001	arg1	migration					971:979	the migration	967:979	the migration of and tube formation in HUVECs treated with ADSC-LPS-exo	967:1037	RNA interference with CREB, AP-1, or NF-κB1 significantly reduced the migration of and tube formation in HUVECs treated with ADSC-LPS-exo.
34445582	8	2	theme	beta	1324:1327	arg1	protein					1332:1338	amyloid beta A4 protein	1316:1338	amyloid beta A4 protein	1316:1338	In addition, proteomic analysis revealed that eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1 may be potential candidates involved in ADSC-LPS-exo-mediated enhanced angiogenesis.
34445582	0	3	theme	Lipopolysaccharide	79:96	arg1	Stimulation					98:108	Lipopolysaccharide Stimulation	79:108	Lipopolysaccharide Stimulation	79:108	Increased Angiogenesis by Exosomes Secreted by Adipose-Derived Stem Cells upon Lipopolysaccharide Stimulation.
34445582	5	4	from	migration	858:866	arg1	HUVECs					893:898	HUVECs	893:898	HUVECs	893:898	The uptake of ADSC-LPS-exo significantly induced the activation of cAMP response element binding protein (CREB), activating protein 1 (AP-1), and nuclear factor-κB (NF-κB) signaling pathways and increased the migration of and tube formation in HUVECs.
34445582	7	5	theme	antibody	1062:1069	arg1	array					1071:1075	an antibody array	1059:1075	an antibody array	1059:1075	An experiment with an antibody array for 25 angiogenesis-related proteins revealed that only interleukin-8 expression was significantly upregulated in HUVECs treated with ADSC-LPS-exo.
34445582	5	6	theme	response	721:728	arg1	CREB					755:758	CREB	755:758	CREB	755:758	The uptake of ADSC-LPS-exo significantly induced the activation of cAMP response element binding protein (CREB), activating protein 1 (AP-1), and nuclear factor-κB (NF-κB) signaling pathways and increased the migration of and tube formation in HUVECs.
34445582	5	6	theme	response	721:728	arg1	protein					746:752	cAMP response element binding protein	716:752	cAMP response element binding protein (CREB)	716:759	The uptake of ADSC-LPS-exo significantly induced the activation of cAMP response element binding protein (CREB), activating protein 1 (AP-1), and nuclear factor-κB (NF-κB) signaling pathways and increased the migration of and tube formation in HUVECs.
34445582	8	7	theme	protein	1332:1338	arg1	substrate					1393:1401	eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1	1271:1403	eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1	1271:1403	In addition, proteomic analysis revealed that eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1 may be potential candidates involved in ADSC-LPS-exo-mediated enhanced angiogenesis.
34445582	5	8	theme	element	730:736	arg1	CREB					755:758	CREB	755:758	CREB	755:758	The uptake of ADSC-LPS-exo significantly induced the activation of cAMP response element binding protein (CREB), activating protein 1 (AP-1), and nuclear factor-κB (NF-κB) signaling pathways and increased the migration of and tube formation in HUVECs.
34445582	5	8	theme	element	730:736	arg1	protein					746:752	cAMP response element binding protein	716:752	cAMP response element binding protein (CREB)	716:759	The uptake of ADSC-LPS-exo significantly induced the activation of cAMP response element binding protein (CREB), activating protein 1 (AP-1), and nuclear factor-κB (NF-κB) signaling pathways and increased the migration of and tube formation in HUVECs.
34445582	6	9	from	migration	971:979	arg1	HUVECs					1006:1011	HUVECs	1006:1011	HUVECs treated with ADSC-LPS-exo	1006:1037	RNA interference with CREB, AP-1, or NF-κB1 significantly reduced the migration of and tube formation in HUVECs treated with ADSC-LPS-exo.
34445582	8	10	theme	A4	1329:1330	arg1	protein					1332:1338	amyloid beta A4 protein	1316:1338	amyloid beta A4 protein	1316:1338	In addition, proteomic analysis revealed that eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1 may be potential candidates involved in ADSC-LPS-exo-mediated enhanced angiogenesis.
34445582	5	11	theme	binding	738:744	arg1	CREB					755:758	CREB	755:758	CREB	755:758	The uptake of ADSC-LPS-exo significantly induced the activation of cAMP response element binding protein (CREB), activating protein 1 (AP-1), and nuclear factor-κB (NF-κB) signaling pathways and increased the migration of and tube formation in HUVECs.
34445582	5	11	theme	binding	738:744	arg1	protein					746:752	cAMP response element binding protein	716:752	cAMP response element binding protein (CREB)	716:759	The uptake of ADSC-LPS-exo significantly induced the activation of cAMP response element binding protein (CREB), activating protein 1 (AP-1), and nuclear factor-κB (NF-κB) signaling pathways and increased the migration of and tube formation in HUVECs.
34445582	8	12	theme	translation	1282:1292	arg1	4E					1312:1313	eukaryotic translation initiation factor 4E	1271:1313	eukaryotic translation initiation factor 4E	1271:1313	In addition, proteomic analysis revealed that eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1 may be potential candidates involved in ADSC-LPS-exo-mediated enhanced angiogenesis.
34445582	5	13	theme	factor-κB	803:811	arg1	pathways					831:838	nuclear factor-κB (NF-κB) signaling pathways	795:838	nuclear factor-κB (NF-κB) signaling pathways	795:838	The uptake of ADSC-LPS-exo significantly induced the activation of cAMP response element binding protein (CREB), activating protein 1 (AP-1), and nuclear factor-κB (NF-κB) signaling pathways and increased the migration of and tube formation in HUVECs.
34445582	8	14	theme	integrin	1341:1348	arg1	beta-1					1350:1355	integrin beta-1	1341:1355	integrin beta-1	1341:1355	In addition, proteomic analysis revealed that eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1 may be potential candidates involved in ADSC-LPS-exo-mediated enhanced angiogenesis.
34445582	3	15	theme	umbilical	443:451	arg1	cells					470:474	human umbilical vein endothelial cells	437:474	human umbilical vein endothelial cells (HUVECs)	437:483	We investigated whether human ADSCs stimulated with lipopolysaccharide (LPS) secrete exosomes (ADSC-LPS-exo) that augment the angiogenesis of human umbilical vein endothelial cells (HUVECs).
34445582	3	15	theme	umbilical	443:451	arg1	HUVECs					477:482	HUVECs	477:482	HUVECs	477:482	We investigated whether human ADSCs stimulated with lipopolysaccharide (LPS) secrete exosomes (ADSC-LPS-exo) that augment the angiogenesis of human umbilical vein endothelial cells (HUVECs).
34445582	5	16	theme	protein	746:752	arg1	activation					702:711	the activation	698:711	the activation of cAMP response element binding protein (CREB), activating protein 1 (AP-1), and nuclear factor-κB (NF-κB) signaling pathways	698:838	The uptake of ADSC-LPS-exo significantly induced the activation of cAMP response element binding protein (CREB), activating protein 1 (AP-1), and nuclear factor-κB (NF-κB) signaling pathways and increased the migration of and tube formation in HUVECs.
34445582	4	17	theme	exosome	498:504	arg1	solution					520:527	ExoQuick-TC exosome precipitation solution	486:527	ExoQuick-TC exosome precipitation solution	486:527	ExoQuick-TC exosome precipitation solution was used to purify exosomes from human ADSC culture media in the presence or absence of 1 µg/mL LPS treatment for 24 h.
34445582	5	18	from	formation	880:888	arg1	HUVECs					893:898	HUVECs	893:898	HUVECs	893:898	The uptake of ADSC-LPS-exo significantly induced the activation of cAMP response element binding protein (CREB), activating protein 1 (AP-1), and nuclear factor-κB (NF-κB) signaling pathways and increased the migration of and tube formation in HUVECs.
34445582	4	19	used	used	533:536	arg2	solution					520:527	ExoQuick-TC exosome precipitation solution	486:527	ExoQuick-TC exosome precipitation solution	486:527	ExoQuick-TC exosome precipitation solution was used to purify exosomes from human ADSC culture media in the presence or absence of 1 µg/mL LPS treatment for 24 h.
34445582	1	20	theme	wound	192:196	arg1	healing					198:204	wound healing	192:204	wound healing	192:204	Exosomes secreted by adipose-derived stem cells (ADSCs) enhance angiogenesis and wound healing.
34445582	7	21	theme	interleukin-8	1133:1145	arg1	expression					1147:1156	only interleukin-8 expression	1128:1156	only interleukin-8 expression	1128:1156	An experiment with an antibody array for 25 angiogenesis-related proteins revealed that only interleukin-8 expression was significantly upregulated in HUVECs treated with ADSC-LPS-exo.
34445582	4	22	theme	ExoQuick-TC	486:496	arg1	solution					520:527	ExoQuick-TC exosome precipitation solution	486:527	ExoQuick-TC exosome precipitation solution	486:527	ExoQuick-TC exosome precipitation solution was used to purify exosomes from human ADSC culture media in the presence or absence of 1 µg/mL LPS treatment for 24 h.
34445582	5	23	theme	ADSC-LPS-exo	663:674	arg1	uptake					653:658	The uptake	649:658	The uptake of ADSC-LPS-exo	649:674	The uptake of ADSC-LPS-exo significantly induced the activation of cAMP response element binding protein (CREB), activating protein 1 (AP-1), and nuclear factor-κB (NF-κB) signaling pathways and increased the migration of and tube formation in HUVECs.
34445582	5	24	theme	pathways	831:838	arg1	activation					702:711	the activation	698:711	the activation of cAMP response element binding protein (CREB), activating protein 1 (AP-1), and nuclear factor-κB (NF-κB) signaling pathways	698:838	The uptake of ADSC-LPS-exo significantly induced the activation of cAMP response element binding protein (CREB), activating protein 1 (AP-1), and nuclear factor-κB (NF-κB) signaling pathways and increased the migration of and tube formation in HUVECs.
34445582	0	25	theme	Increased	0:8	arg1	Angiogenesis					10:21	Increased Angiogenesis	0:21	Increased Angiogenesis by Exosomes	0:33	Increased Angiogenesis by Exosomes Secreted by Adipose-Derived Stem Cells upon Lipopolysaccharide Stimulation.
34445582	4	26	theme	ADSC	568:571	arg1	media					581:585	human ADSC culture media	562:585	human ADSC culture media	562:585	ExoQuick-TC exosome precipitation solution was used to purify exosomes from human ADSC culture media in the presence or absence of 1 µg/mL LPS treatment for 24 h.
34445582	5	27	theme	signaling	821:829	arg1	pathways					831:838	nuclear factor-κB (NF-κB) signaling pathways	795:838	nuclear factor-κB (NF-κB) signaling pathways	795:838	The uptake of ADSC-LPS-exo significantly induced the activation of cAMP response element binding protein (CREB), activating protein 1 (AP-1), and nuclear factor-κB (NF-κB) signaling pathways and increased the migration of and tube formation in HUVECs.
34445582	8	28	theme	proteomic	1238:1246	arg1	analysis					1248:1255	proteomic analysis	1238:1255	proteomic analysis	1238:1255	In addition, proteomic analysis revealed that eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1 may be potential candidates involved in ADSC-LPS-exo-mediated enhanced angiogenesis.
34445582	3	29	theme	human	437:441	arg1	cells					470:474	human umbilical vein endothelial cells	437:474	human umbilical vein endothelial cells (HUVECs)	437:483	We investigated whether human ADSCs stimulated with lipopolysaccharide (LPS) secrete exosomes (ADSC-LPS-exo) that augment the angiogenesis of human umbilical vein endothelial cells (HUVECs).
34445582	3	29	theme	human	437:441	arg1	HUVECs					477:482	HUVECs	477:482	HUVECs	477:482	We investigated whether human ADSCs stimulated with lipopolysaccharide (LPS) secrete exosomes (ADSC-LPS-exo) that augment the angiogenesis of human umbilical vein endothelial cells (HUVECs).
34445582	4	30	theme	human	562:566	arg1	media					581:585	human ADSC culture media	562:585	human ADSC culture media	562:585	ExoQuick-TC exosome precipitation solution was used to purify exosomes from human ADSC culture media in the presence or absence of 1 µg/mL LPS treatment for 24 h.
34445582	6	31	theme	tube	988:991	arg1	formation					993:1001	and tube formation	984:1001	formation	993:1001	RNA interference with CREB, AP-1, or NF-κB1 significantly reduced the migration of and tube formation in HUVECs treated with ADSC-LPS-exo.
34445582	4	32	theme	treatment	629:637	arg1	presence					594:601	presence	594:601	presence	594:601	ExoQuick-TC exosome precipitation solution was used to purify exosomes from human ADSC culture media in the presence or absence of 1 µg/mL LPS treatment for 24 h.
34445582	4	32	theme	treatment	629:637	arg1	absence					606:612	absence	606:612	absence	606:612	ExoQuick-TC exosome precipitation solution was used to purify exosomes from human ADSC culture media in the presence or absence of 1 µg/mL LPS treatment for 24 h.
34445582	7	33	theme	only	1128:1131	arg1	expression					1147:1156	only interleukin-8 expression	1128:1156	only interleukin-8 expression	1128:1156	An experiment with an antibody array for 25 angiogenesis-related proteins revealed that only interleukin-8 expression was significantly upregulated in HUVECs treated with ADSC-LPS-exo.
34445582	8	34	theme	amyloid	1316:1322	arg1	protein					1332:1338	amyloid beta A4 protein	1316:1338	amyloid beta A4 protein	1316:1338	In addition, proteomic analysis revealed that eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1 may be potential candidates involved in ADSC-LPS-exo-mediated enhanced angiogenesis.
34445582	4	35	theme	LPS	625:627	arg1	treatment					629:637	1 µg/mL LPS treatment	617:637	1 µg/mL LPS treatment for 24 h	617:646	ExoQuick-TC exosome precipitation solution was used to purify exosomes from human ADSC culture media in the presence or absence of 1 µg/mL LPS treatment for 24 h.
34445582	5	36	theme	activating	762:771	arg1	protein					773:779	activating protein 1	762:781	activating protein 1 (AP-1)	762:788	The uptake of ADSC-LPS-exo significantly induced the activation of cAMP response element binding protein (CREB), activating protein 1 (AP-1), and nuclear factor-κB (NF-κB) signaling pathways and increased the migration of and tube formation in HUVECs.
34445582	5	36	theme	activating	762:771	arg1	AP-1					784:787	AP-1	784:787	AP-1	784:787	The uptake of ADSC-LPS-exo significantly induced the activation of cAMP response element binding protein (CREB), activating protein 1 (AP-1), and nuclear factor-κB (NF-κB) signaling pathways and increased the migration of and tube formation in HUVECs.
34445582	4	37	theme	culture	573:579	arg1	media					581:585	human ADSC culture media	562:585	human ADSC culture media	562:585	ExoQuick-TC exosome precipitation solution was used to purify exosomes from human ADSC culture media in the presence or absence of 1 µg/mL LPS treatment for 24 h.
34445582	4	38	dep	presence	594:601	arg1	the					590:592	the	590:592	the	590:592	ExoQuick-TC exosome precipitation solution was used to purify exosomes from human ADSC culture media in the presence or absence of 1 µg/mL LPS treatment for 24 h.
34445582	8	39	theme	botulinum	1377:1385	arg1	toxin					1387:1391	ras-related C3 botulinum toxin	1362:1391	ras-related C3 botulinum toxin	1362:1391	In addition, proteomic analysis revealed that eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1 may be potential candidates involved in ADSC-LPS-exo-mediated enhanced angiogenesis.
34445582	3	40	theme	endothelial	458:468	arg1	cells					470:474	human umbilical vein endothelial cells	437:474	human umbilical vein endothelial cells (HUVECs)	437:483	We investigated whether human ADSCs stimulated with lipopolysaccharide (LPS) secrete exosomes (ADSC-LPS-exo) that augment the angiogenesis of human umbilical vein endothelial cells (HUVECs).
34445582	3	40	theme	endothelial	458:468	arg1	HUVECs					477:482	HUVECs	477:482	HUVECs	477:482	We investigated whether human ADSCs stimulated with lipopolysaccharide (LPS) secrete exosomes (ADSC-LPS-exo) that augment the angiogenesis of human umbilical vein endothelial cells (HUVECs).
34445582	5	41	theme	protein	773:779	arg1	activation					702:711	the activation	698:711	the activation of cAMP response element binding protein (CREB), activating protein 1 (AP-1), and nuclear factor-κB (NF-κB) signaling pathways	698:838	The uptake of ADSC-LPS-exo significantly induced the activation of cAMP response element binding protein (CREB), activating protein 1 (AP-1), and nuclear factor-κB (NF-κB) signaling pathways and increased the migration of and tube formation in HUVECs.
34445582	5	42	theme	cAMP	716:719	arg1	CREB					755:758	CREB	755:758	CREB	755:758	The uptake of ADSC-LPS-exo significantly induced the activation of cAMP response element binding protein (CREB), activating protein 1 (AP-1), and nuclear factor-κB (NF-κB) signaling pathways and increased the migration of and tube formation in HUVECs.
34445582	5	42	theme	cAMP	716:719	arg1	protein					746:752	cAMP response element binding protein	716:752	cAMP response element binding protein (CREB)	716:759	The uptake of ADSC-LPS-exo significantly induced the activation of cAMP response element binding protein (CREB), activating protein 1 (AP-1), and nuclear factor-κB (NF-κB) signaling pathways and increased the migration of and tube formation in HUVECs.
34445582	7	43	theme	angiogenesis-related	1084:1103	arg1	proteins					1105:1112	25 angiogenesis-related proteins	1081:1112	25 angiogenesis-related proteins	1081:1112	An experiment with an antibody array for 25 angiogenesis-related proteins revealed that only interleukin-8 expression was significantly upregulated in HUVECs treated with ADSC-LPS-exo.
34445582	5	44	theme	tube	875:878	arg1	formation					880:888	tube formation	875:888	tube formation in HUVECs	875:898	The uptake of ADSC-LPS-exo significantly induced the activation of cAMP response element binding protein (CREB), activating protein 1 (AP-1), and nuclear factor-κB (NF-κB) signaling pathways and increased the migration of and tube formation in HUVECs.
34445582	3	45	theme	cells	470:474	arg1	angiogenesis					421:432	the angiogenesis	417:432	the angiogenesis of human umbilical vein endothelial cells (HUVECs)	417:483	We investigated whether human ADSCs stimulated with lipopolysaccharide (LPS) secrete exosomes (ADSC-LPS-exo) that augment the angiogenesis of human umbilical vein endothelial cells (HUVECs).
34445582	0	46	theme	Stem	63:66	arg1	Cells					68:72	Adipose-Derived Stem Cells	47:72	Adipose-Derived Stem Cells	47:72	Increased Angiogenesis by Exosomes Secreted by Adipose-Derived Stem Cells upon Lipopolysaccharide Stimulation.
34445582	6	47	theme	RNA	901:903	arg1	interference					905:916	RNA interference	901:916	RNA interference with CREB, AP-1, or NF-κB1	901:943	RNA interference with CREB, AP-1, or NF-κB1 significantly reduced the migration of and tube formation in HUVECs treated with ADSC-LPS-exo.
34445582	8	48	theme	beta-1	1350:1355	arg1	substrate					1393:1401	eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1	1271:1403	eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1	1271:1403	In addition, proteomic analysis revealed that eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1 may be potential candidates involved in ADSC-LPS-exo-mediated enhanced angiogenesis.
34445582	1	49	theme	adipose-derived	132:146	arg1	cells					153:157	adipose-derived stem cells	132:157	adipose-derived stem cells (ADSCs)	132:165	Exosomes secreted by adipose-derived stem cells (ADSCs) enhance angiogenesis and wound healing.
34445582	1	49	theme	adipose-derived	132:146	arg1	ADSCs					160:164	ADSCs	160:164	ADSCs	160:164	Exosomes secreted by adipose-derived stem cells (ADSCs) enhance angiogenesis and wound healing.
34445582	0	50	theme	Adipose-Derived	47:61	arg1	Cells					68:72	Adipose-Derived Stem Cells	47:72	Adipose-Derived Stem Cells	47:72	Increased Angiogenesis by Exosomes Secreted by Adipose-Derived Stem Cells upon Lipopolysaccharide Stimulation.
34445582	3	51	theme	human	319:323	arg1	ADSCs					325:329	human ADSCs	319:329	human ADSCs stimulated with lipopolysaccharide (LPS)	319:370	We investigated whether human ADSCs stimulated with lipopolysaccharide (LPS) secrete exosomes (ADSC-LPS-exo) that augment the angiogenesis of human umbilical vein endothelial cells (HUVECs).
34445582	2	52	theme	clinical	219:226	arg1	settings					228:235	clinical settings	219:235	clinical settings	219:235	However, in clinical settings, wounds may be infected by various bacteria or pathogens.
34445582	8	53	theme	toxin	1387:1391	arg1	substrate					1393:1401	eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1	1271:1403	eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1	1271:1403	In addition, proteomic analysis revealed that eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1 may be potential candidates involved in ADSC-LPS-exo-mediated enhanced angiogenesis.
34445582	1	54	theme	stem	148:151	arg1	cells					153:157	adipose-derived stem cells	132:157	adipose-derived stem cells (ADSCs)	132:165	Exosomes secreted by adipose-derived stem cells (ADSCs) enhance angiogenesis and wound healing.
34445582	1	54	theme	stem	148:151	arg1	ADSCs					160:164	ADSCs	160:164	ADSCs	160:164	Exosomes secreted by adipose-derived stem cells (ADSCs) enhance angiogenesis and wound healing.
34445582	1	55	link	adipose-derived	132:146	arg1	cells					153:157	adipose-derived stem cells	132:157	adipose-derived stem cells (ADSCs)	132:165	Exosomes secreted by adipose-derived stem cells (ADSCs) enhance angiogenesis and wound healing.
34445582	1	55	link	adipose-derived	132:146	arg1	ADSCs					160:164	ADSCs	160:164	ADSCs	160:164	Exosomes secreted by adipose-derived stem cells (ADSCs) enhance angiogenesis and wound healing.
34445582	8	56	theme	enhanced	1467:1474	arg1	angiogenesis					1476:1487	ADSC-LPS-exo-mediated enhanced angiogenesis	1445:1487	ADSC-LPS-exo-mediated enhanced angiogenesis	1445:1487	In addition, proteomic analysis revealed that eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1 may be potential candidates involved in ADSC-LPS-exo-mediated enhanced angiogenesis.
34445582	2	57	theme	various	264:270	arg1	bacteria					272:279	various bacteria	264:279	various bacteria	264:279	However, in clinical settings, wounds may be infected by various bacteria or pathogens.
34445582	8	58	theme	C3	1374:1375	arg1	toxin					1387:1391	ras-related C3 botulinum toxin	1362:1391	ras-related C3 botulinum toxin	1362:1391	In addition, proteomic analysis revealed that eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1 may be potential candidates involved in ADSC-LPS-exo-mediated enhanced angiogenesis.
34445582	7	59	with	experiment	1043:1052	arg1	array					1071:1075	an antibody array	1059:1075	an antibody array	1059:1075	An experiment with an antibody array for 25 angiogenesis-related proteins revealed that only interleukin-8 expression was significantly upregulated in HUVECs treated with ADSC-LPS-exo.
34445582	6	60	with	interference	905:916	arg1	NF-κB1					938:943	NF-κB1	938:943	NF-κB1	938:943	RNA interference with CREB, AP-1, or NF-κB1 significantly reduced the migration of and tube formation in HUVECs treated with ADSC-LPS-exo.
34445582	6	60	with	interference	905:916	arg1	CREB					923:926	CREB	923:926	CREB	923:926	RNA interference with CREB, AP-1, or NF-κB1 significantly reduced the migration of and tube formation in HUVECs treated with ADSC-LPS-exo.
34445582	6	60	with	interference	905:916	arg1	AP-1					929:932	AP-1	929:932	AP-1	929:932	RNA interference with CREB, AP-1, or NF-κB1 significantly reduced the migration of and tube formation in HUVECs treated with ADSC-LPS-exo.
34445582	8	61	theme	initiation	1294:1303	arg1	4E					1312:1313	eukaryotic translation initiation factor 4E	1271:1313	eukaryotic translation initiation factor 4E	1271:1313	In addition, proteomic analysis revealed that eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1 may be potential candidates involved in ADSC-LPS-exo-mediated enhanced angiogenesis.
34445582	8	62	theme	factor	1305:1310	arg1	4E					1312:1313	eukaryotic translation initiation factor 4E	1271:1313	eukaryotic translation initiation factor 4E	1271:1313	In addition, proteomic analysis revealed that eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1 may be potential candidates involved in ADSC-LPS-exo-mediated enhanced angiogenesis.
34445582	8	63	theme	ADSC-LPS-exo-mediated	1445:1465	arg1	angiogenesis					1476:1487	ADSC-LPS-exo-mediated enhanced angiogenesis	1445:1487	ADSC-LPS-exo-mediated enhanced angiogenesis	1445:1487	In addition, proteomic analysis revealed that eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1 may be potential candidates involved in ADSC-LPS-exo-mediated enhanced angiogenesis.
34445582	8	64	theme	ras-related	1362:1372	arg1	toxin					1387:1391	ras-related C3 botulinum toxin	1362:1391	ras-related C3 botulinum toxin	1362:1391	In addition, proteomic analysis revealed that eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1 may be potential candidates involved in ADSC-LPS-exo-mediated enhanced angiogenesis.
34445582	8	65	theme	potential	1412:1420	arg1	candidates					1422:1431	potential candidates	1412:1431	potential candidates involved in ADSC-LPS-exo-mediated enhanced angiogenesis	1412:1487	In addition, proteomic analysis revealed that eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1 may be potential candidates involved in ADSC-LPS-exo-mediated enhanced angiogenesis.
34445582	5	66	theme	nuclear	795:801	arg1	NF-κB					814:818	NF-κB	814:818	NF-κB	814:818	The uptake of ADSC-LPS-exo significantly induced the activation of cAMP response element binding protein (CREB), activating protein 1 (AP-1), and nuclear factor-κB (NF-κB) signaling pathways and increased the migration of and tube formation in HUVECs.
34445582	5	66	theme	nuclear	795:801	arg1	factor-κB					803:811	nuclear factor-κB	795:811	nuclear factor-κB (NF-κB) signaling pathways	795:838	The uptake of ADSC-LPS-exo significantly induced the activation of cAMP response element binding protein (CREB), activating protein 1 (AP-1), and nuclear factor-κB (NF-κB) signaling pathways and increased the migration of and tube formation in HUVECs.
34445582	3	67	theme	vein	453:456	arg1	cells					470:474	human umbilical vein endothelial cells	437:474	human umbilical vein endothelial cells (HUVECs)	437:483	We investigated whether human ADSCs stimulated with lipopolysaccharide (LPS) secrete exosomes (ADSC-LPS-exo) that augment the angiogenesis of human umbilical vein endothelial cells (HUVECs).
34445582	3	67	theme	vein	453:456	arg1	HUVECs					477:482	HUVECs	477:482	HUVECs	477:482	We investigated whether human ADSCs stimulated with lipopolysaccharide (LPS) secrete exosomes (ADSC-LPS-exo) that augment the angiogenesis of human umbilical vein endothelial cells (HUVECs).
34445582	4	68	theme	µg/mL	619:623	arg1	treatment					629:637	1 µg/mL LPS treatment	617:637	1 µg/mL LPS treatment for 24 h	617:646	ExoQuick-TC exosome precipitation solution was used to purify exosomes from human ADSC culture media in the presence or absence of 1 µg/mL LPS treatment for 24 h.
34445582	8	69	theme	4E	1312:1313	arg1	substrate					1393:1401	eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1	1271:1403	eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1	1271:1403	In addition, proteomic analysis revealed that eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1 may be potential candidates involved in ADSC-LPS-exo-mediated enhanced angiogenesis.
34445582	8	70	theme	eukaryotic	1271:1280	arg1	4E					1312:1313	eukaryotic translation initiation factor 4E	1271:1313	eukaryotic translation initiation factor 4E	1271:1313	In addition, proteomic analysis revealed that eukaryotic translation initiation factor 4E, amyloid beta A4 protein, integrin beta-1, and ras-related C3 botulinum toxin substrate 1 may be potential candidates involved in ADSC-LPS-exo-mediated enhanced angiogenesis.
34405464	7	0	theme	function	1203:1210	arg1	intergenerationally					1212:1230	metabolic function intergenerationally	1193:1230	metabolic function intergenerationally	1193:1230	Our study identifies paternal HP diet as a modulator of gut microbial composition, epigenetic markers, and metabolic function intergenerationally.
34405464	5	1	from	clustering	850:859	arg1	fathers					957:963	HP fathers	954:963	HP fathers	954:963	Further, using 16S rRNA gene sequencing to assess gut microbial composition, we observed increased alpha diversity, distinct bacterial clustering, and increased abundance of Bifidobacterium, Akkermansia, Bacteroides, and Marvinbryantia in HP fathers and/or male and female adult offspring.
34405464	5	1	from	clustering	850:859	arg1	offspring					994:1002	male and female adult offspring	972:1002	male and female adult offspring	972:1002	Further, using 16S rRNA gene sequencing to assess gut microbial composition, we observed increased alpha diversity, distinct bacterial clustering, and increased abundance of Bifidobacterium, Akkermansia, Bacteroides, and Marvinbryantia in HP fathers and/or male and female adult offspring.
34405464	5	2	theme	16S	730:732	arg1	sequencing					744:753	16S rRNA gene sequencing	730:753	16S rRNA gene sequencing	730:753	Further, using 16S rRNA gene sequencing to assess gut microbial composition, we observed increased alpha diversity, distinct bacterial clustering, and increased abundance of Bifidobacterium, Akkermansia, Bacteroides, and Marvinbryantia in HP fathers and/or male and female adult offspring.
34405464	6	3	theme	3b	1039:1040	arg1	expression					1042:1051	DNMT1and 3b expression	1030:1051	DNMT1and 3b expression	1030:1051	At the epigenetic level, DNMT1and 3b expression was altered intergenerationally.
34405464	2	4	theme	epigenetic	341:350	arg1	changes					352:358	epigenetic changes	341:358	epigenetic changes	341:358	However, the contribution of a pre-conception paternal high protein (HP) diet to offspring metabolism, gut microbiota, and epigenetic changes remains unclear.
34405464	3	5	from	intake	407:412	arg1	rats					432:435	Sprague Dawley rats	417:435	Sprague Dawley rats	417:435	Here we show that paternal HP intake in Sprague Dawley rats programs protective metabolic outcomes in offspring.
34405464	7	6	theme	paternal	1107:1114	arg1	diet					1119:1122	paternal HP diet	1107:1122	paternal HP diet	1107:1122	Our study identifies paternal HP diet as a modulator of gut microbial composition, epigenetic markers, and metabolic function intergenerationally.
34405464	7	6	theme	paternal	1107:1114	arg1	modulator					1129:1137	a modulator	1127:1137	a modulator of gut microbial composition, epigenetic markers, and metabolic function intergenerationally	1127:1230	Our study identifies paternal HP diet as a modulator of gut microbial composition, epigenetic markers, and metabolic function intergenerationally.
34405464	2	7	theme	diet	291:294	arg1	contribution					231:242	the contribution	227:242	the contribution of a pre-conception paternal high protein (HP) diet to offspring metabolism, gut microbiota, and epigenetic changes	227:358	However, the contribution of a pre-conception paternal high protein (HP) diet to offspring metabolism, gut microbiota, and epigenetic changes remains unclear.
34405464	5	8	theme	female	981:986	arg1	offspring					994:1002	male and female adult offspring	972:1002	male and female adult offspring	972:1002	Further, using 16S rRNA gene sequencing to assess gut microbial composition, we observed increased alpha diversity, distinct bacterial clustering, and increased abundance of Bifidobacterium, Akkermansia, Bacteroides, and Marvinbryantia in HP fathers and/or male and female adult offspring.
34405464	7	9	theme	metabolic	1193:1201	arg1	intergenerationally					1212:1230	metabolic function intergenerationally	1193:1230	metabolic function intergenerationally	1193:1230	Our study identifies paternal HP diet as a modulator of gut microbial composition, epigenetic markers, and metabolic function intergenerationally.
34405464	7	10	theme	HP	1116:1117	arg1	diet					1119:1122	paternal HP diet	1107:1122	paternal HP diet	1107:1122	Our study identifies paternal HP diet as a modulator of gut microbial composition, epigenetic markers, and metabolic function intergenerationally.
34405464	7	10	theme	HP	1116:1117	arg1	modulator					1129:1137	a modulator	1127:1137	a modulator of gut microbial composition, epigenetic markers, and metabolic function intergenerationally	1127:1230	Our study identifies paternal HP diet as a modulator of gut microbial composition, epigenetic markers, and metabolic function intergenerationally.
34405464	4	11	theme	insulin	574:580	arg1	sensitivity					582:592	insulin sensitivity	574:592	insulin sensitivity	574:592	Compared to paternal high fat/sucrose (HF/S), HP diet improved body composition and insulin sensitivity and improved circulating satiety hormones and cecal short-chain fatty acids compared to HF/S and control diet (P < .05).
34405464	0	12	theme	microbiota	97:106	arg1	intergenerationally					108:126	gut microbiota intergenerationally	93:126	gut microbiota intergenerationally	93:126	Paternal high protein diet modulates body composition, insulin sensitivity, epigenetics, and gut microbiota intergenerationally in rats.
34405464	0	13	from	composition	42:52	arg1	rats					131:134	rats	131:134	rats	131:134	Paternal high protein diet modulates body composition, insulin sensitivity, epigenetics, and gut microbiota intergenerationally in rats.
34405464	5	14	from	abundance	876:884	arg1	fathers					957:963	HP fathers	954:963	HP fathers	954:963	Further, using 16S rRNA gene sequencing to assess gut microbial composition, we observed increased alpha diversity, distinct bacterial clustering, and increased abundance of Bifidobacterium, Akkermansia, Bacteroides, and Marvinbryantia in HP fathers and/or male and female adult offspring.
34405464	5	14	from	abundance	876:884	arg1	offspring					994:1002	male and female adult offspring	972:1002	male and female adult offspring	972:1002	Further, using 16S rRNA gene sequencing to assess gut microbial composition, we observed increased alpha diversity, distinct bacterial clustering, and increased abundance of Bifidobacterium, Akkermansia, Bacteroides, and Marvinbryantia in HP fathers and/or male and female adult offspring.
34405464	2	15	theme	HP	287:288	arg1	diet					291:294	a pre-conception paternal high protein (HP) diet	247:294	a pre-conception paternal high protein (HP) diet	247:294	However, the contribution of a pre-conception paternal high protein (HP) diet to offspring metabolism, gut microbiota, and epigenetic changes remains unclear.
34405464	1	16	theme	paternal	173:180	arg1	diet					182:185	paternal diet	173:185	paternal diet	173:185	Mounting evidence demonstrates that paternal diet programs offspring metabolism.
34405464	0	17	theme	gut	93:95	arg1	intergenerationally					108:126	gut microbiota intergenerationally	93:126	gut microbiota intergenerationally	93:126	Paternal high protein diet modulates body composition, insulin sensitivity, epigenetics, and gut microbiota intergenerationally in rats.
34405464	5	18	theme	Bifidobacterium	889:903	arg1	clustering					850:859	distinct bacterial clustering	831:859	distinct bacterial clustering	831:859	Further, using 16S rRNA gene sequencing to assess gut microbial composition, we observed increased alpha diversity, distinct bacterial clustering, and increased abundance of Bifidobacterium, Akkermansia, Bacteroides, and Marvinbryantia in HP fathers and/or male and female adult offspring.
34405464	5	18	theme	Bifidobacterium	889:903	arg1	diversity					820:828	increased alpha diversity	804:828	increased alpha diversity	804:828	Further, using 16S rRNA gene sequencing to assess gut microbial composition, we observed increased alpha diversity, distinct bacterial clustering, and increased abundance of Bifidobacterium, Akkermansia, Bacteroides, and Marvinbryantia in HP fathers and/or male and female adult offspring.
34405464	5	18	theme	Bifidobacterium	889:903	arg1	abundance					876:884	increased abundance	866:884	increased abundance of Bifidobacterium, Akkermansia, Bacteroides, and Marvinbryantia	866:949	Further, using 16S rRNA gene sequencing to assess gut microbial composition, we observed increased alpha diversity, distinct bacterial clustering, and increased abundance of Bifidobacterium, Akkermansia, Bacteroides, and Marvinbryantia in HP fathers and/or male and female adult offspring.
34405464	3	19	theme	HP	404:405	arg1	intake					407:412	paternal HP intake	395:412	paternal HP intake in Sprague Dawley rats	395:435	Here we show that paternal HP intake in Sprague Dawley rats programs protective metabolic outcomes in offspring.
34405464	5	20	theme	male	972:975	arg1	offspring					994:1002	male and female adult offspring	972:1002	male and female adult offspring	972:1002	Further, using 16S rRNA gene sequencing to assess gut microbial composition, we observed increased alpha diversity, distinct bacterial clustering, and increased abundance of Bifidobacterium, Akkermansia, Bacteroides, and Marvinbryantia in HP fathers and/or male and female adult offspring.
34405464	4	21	theme	fatty	658:662	arg1	acids					664:668	cecal short-chain fatty acids	640:668	cecal short-chain fatty acids	640:668	Compared to paternal high fat/sucrose (HF/S), HP diet improved body composition and insulin sensitivity and improved circulating satiety hormones and cecal short-chain fatty acids compared to HF/S and control diet (P < .05).
34405464	0	22	theme	high	9:12	arg1	diet					22:25	Paternal high protein diet	0:25	Paternal high protein diet	0:25	Paternal high protein diet modulates body composition, insulin sensitivity, epigenetics, and gut microbiota intergenerationally in rats.
34405464	7	23	theme	intergenerationally	1212:1230	arg1	diet					1119:1122	paternal HP diet	1107:1122	paternal HP diet	1107:1122	Our study identifies paternal HP diet as a modulator of gut microbial composition, epigenetic markers, and metabolic function intergenerationally.
34405464	7	23	theme	intergenerationally	1212:1230	arg1	modulator					1129:1137	a modulator	1127:1137	a modulator of gut microbial composition, epigenetic markers, and metabolic function intergenerationally	1127:1230	Our study identifies paternal HP diet as a modulator of gut microbial composition, epigenetic markers, and metabolic function intergenerationally.
34405464	5	24	theme	adult	988:992	arg1	offspring					994:1002	male and female adult offspring	972:1002	male and female adult offspring	972:1002	Further, using 16S rRNA gene sequencing to assess gut microbial composition, we observed increased alpha diversity, distinct bacterial clustering, and increased abundance of Bifidobacterium, Akkermansia, Bacteroides, and Marvinbryantia in HP fathers and/or male and female adult offspring.
34405464	0	25	theme	Paternal	0:7	arg1	diet					22:25	Paternal high protein diet	0:25	Paternal high protein diet	0:25	Paternal high protein diet modulates body composition, insulin sensitivity, epigenetics, and gut microbiota intergenerationally in rats.
34405464	7	26	theme	gut	1142:1144	arg1	composition					1156:1166	gut microbial composition	1142:1166	gut microbial composition	1142:1166	Our study identifies paternal HP diet as a modulator of gut microbial composition, epigenetic markers, and metabolic function intergenerationally.
34405464	5	27	theme	bacterial	840:848	arg1	clustering					850:859	distinct bacterial clustering	831:859	distinct bacterial clustering	831:859	Further, using 16S rRNA gene sequencing to assess gut microbial composition, we observed increased alpha diversity, distinct bacterial clustering, and increased abundance of Bifidobacterium, Akkermansia, Bacteroides, and Marvinbryantia in HP fathers and/or male and female adult offspring.
34405464	6	28	theme	DNMT1and	1030:1037	arg1	expression					1042:1051	DNMT1and 3b expression	1030:1051	DNMT1and 3b expression	1030:1051	At the epigenetic level, DNMT1and 3b expression was altered intergenerationally.
34405464	1	29	theme	offspring	196:204	arg1	metabolism					206:215	offspring metabolism	196:215	offspring metabolism	196:215	Mounting evidence demonstrates that paternal diet programs offspring metabolism.
34405464	7	30	theme	microbial	1146:1154	arg1	composition					1156:1166	gut microbial composition	1142:1166	gut microbial composition	1142:1166	Our study identifies paternal HP diet as a modulator of gut microbial composition, epigenetic markers, and metabolic function intergenerationally.
34405464	4	31	theme	HP	536:537	arg1	diet					539:542	HP diet	536:542	HP diet	536:542	Compared to paternal high fat/sucrose (HF/S), HP diet improved body composition and insulin sensitivity and improved circulating satiety hormones and cecal short-chain fatty acids compared to HF/S and control diet (P < .05).
34405464	2	32	theme	offspring	299:307	arg1	metabolism					309:318	offspring metabolism	299:318	offspring metabolism	299:318	However, the contribution of a pre-conception paternal high protein (HP) diet to offspring metabolism, gut microbiota, and epigenetic changes remains unclear.
34405464	3	33	theme	metabolic	457:465	arg1	outcomes					467:474	protective metabolic outcomes	446:474	protective metabolic outcomes	446:474	Here we show that paternal HP intake in Sprague Dawley rats programs protective metabolic outcomes in offspring.
34405464	0	34	theme	protein	14:20	arg1	diet					22:25	Paternal high protein diet	0:25	Paternal high protein diet	0:25	Paternal high protein diet modulates body composition, insulin sensitivity, epigenetics, and gut microbiota intergenerationally in rats.
34405464	4	35	theme	body	553:556	arg1	composition					558:568	body composition	553:568	body composition	553:568	Compared to paternal high fat/sucrose (HF/S), HP diet improved body composition and insulin sensitivity and improved circulating satiety hormones and cecal short-chain fatty acids compared to HF/S and control diet (P < .05).
34405464	0	36	from	epigenetics	76:86	arg1	rats					131:134	rats	131:134	rats	131:134	Paternal high protein diet modulates body composition, insulin sensitivity, epigenetics, and gut microbiota intergenerationally in rats.
34405464	4	37	theme	control	691:697	arg1	P < .05					705:711	P < .05	705:711	P < .05	705:711	Compared to paternal high fat/sucrose (HF/S), HP diet improved body composition and insulin sensitivity and improved circulating satiety hormones and cecal short-chain fatty acids compared to HF/S and control diet (P < .05).
34405464	4	37	theme	control	691:697	arg1	diet					699:702	control diet	691:702	control diet (P < .05)	691:712	Compared to paternal high fat/sucrose (HF/S), HP diet improved body composition and insulin sensitivity and improved circulating satiety hormones and cecal short-chain fatty acids compared to HF/S and control diet (P < .05).
34405464	5	38	theme	increased	866:874	arg1	abundance					876:884	increased abundance	866:884	increased abundance of Bifidobacterium, Akkermansia, Bacteroides, and Marvinbryantia	866:949	Further, using 16S rRNA gene sequencing to assess gut microbial composition, we observed increased alpha diversity, distinct bacterial clustering, and increased abundance of Bifidobacterium, Akkermansia, Bacteroides, and Marvinbryantia in HP fathers and/or male and female adult offspring.
34405464	0	39	theme	body	37:40	arg1	composition					42:52	body composition	37:52	body composition	37:52	Paternal high protein diet modulates body composition, insulin sensitivity, epigenetics, and gut microbiota intergenerationally in rats.
34405464	5	40	theme	HP	954:955	arg1	fathers					957:963	HP fathers	954:963	HP fathers	954:963	Further, using 16S rRNA gene sequencing to assess gut microbial composition, we observed increased alpha diversity, distinct bacterial clustering, and increased abundance of Bifidobacterium, Akkermansia, Bacteroides, and Marvinbryantia in HP fathers and/or male and female adult offspring.
34405464	7	41	theme	markers	1180:1186	arg1	diet					1119:1122	paternal HP diet	1107:1122	paternal HP diet	1107:1122	Our study identifies paternal HP diet as a modulator of gut microbial composition, epigenetic markers, and metabolic function intergenerationally.
34405464	7	41	theme	markers	1180:1186	arg1	modulator					1129:1137	a modulator	1127:1137	a modulator of gut microbial composition, epigenetic markers, and metabolic function intergenerationally	1127:1230	Our study identifies paternal HP diet as a modulator of gut microbial composition, epigenetic markers, and metabolic function intergenerationally.
34405464	7	42	theme	epigenetic	1169:1178	arg1	markers					1180:1186	epigenetic markers	1169:1186	epigenetic markers	1169:1186	Our study identifies paternal HP diet as a modulator of gut microbial composition, epigenetic markers, and metabolic function intergenerationally.
34405464	4	43	theme	short-chain	646:656	arg1	acids					664:668	cecal short-chain fatty acids	640:668	cecal short-chain fatty acids	640:668	Compared to paternal high fat/sucrose (HF/S), HP diet improved body composition and insulin sensitivity and improved circulating satiety hormones and cecal short-chain fatty acids compared to HF/S and control diet (P < .05).
34405464	3	44	theme	Dawley	425:430	arg1	rats					432:435	Sprague Dawley rats	417:435	Sprague Dawley rats	417:435	Here we show that paternal HP intake in Sprague Dawley rats programs protective metabolic outcomes in offspring.
34405464	5	45	theme	gut	765:767	arg1	composition					779:789	gut microbial composition	765:789	gut microbial composition	765:789	Further, using 16S rRNA gene sequencing to assess gut microbial composition, we observed increased alpha diversity, distinct bacterial clustering, and increased abundance of Bifidobacterium, Akkermansia, Bacteroides, and Marvinbryantia in HP fathers and/or male and female adult offspring.
34405464	0	46	from	sensitivity	63:73	arg1	rats					131:134	rats	131:134	rats	131:134	Paternal high protein diet modulates body composition, insulin sensitivity, epigenetics, and gut microbiota intergenerationally in rats.
34405464	5	47	theme	distinct	831:838	arg1	clustering					850:859	distinct bacterial clustering	831:859	distinct bacterial clustering	831:859	Further, using 16S rRNA gene sequencing to assess gut microbial composition, we observed increased alpha diversity, distinct bacterial clustering, and increased abundance of Bifidobacterium, Akkermansia, Bacteroides, and Marvinbryantia in HP fathers and/or male and female adult offspring.
34405464	3	48	theme	protective	446:455	arg1	outcomes					467:474	protective metabolic outcomes	446:474	protective metabolic outcomes	446:474	Here we show that paternal HP intake in Sprague Dawley rats programs protective metabolic outcomes in offspring.
34405464	5	49	theme	microbial	769:777	arg1	composition					779:789	gut microbial composition	765:789	gut microbial composition	765:789	Further, using 16S rRNA gene sequencing to assess gut microbial composition, we observed increased alpha diversity, distinct bacterial clustering, and increased abundance of Bifidobacterium, Akkermansia, Bacteroides, and Marvinbryantia in HP fathers and/or male and female adult offspring.
34405464	5	50	theme	increased	804:812	arg1	diversity					820:828	increased alpha diversity	804:828	increased alpha diversity	804:828	Further, using 16S rRNA gene sequencing to assess gut microbial composition, we observed increased alpha diversity, distinct bacterial clustering, and increased abundance of Bifidobacterium, Akkermansia, Bacteroides, and Marvinbryantia in HP fathers and/or male and female adult offspring.
34405464	5	51	theme	gene	739:742	arg1	sequencing					744:753	16S rRNA gene sequencing	730:753	16S rRNA gene sequencing	730:753	Further, using 16S rRNA gene sequencing to assess gut microbial composition, we observed increased alpha diversity, distinct bacterial clustering, and increased abundance of Bifidobacterium, Akkermansia, Bacteroides, and Marvinbryantia in HP fathers and/or male and female adult offspring.
34405464	4	52	theme	circulating	607:617	arg1	hormones					627:634	circulating satiety hormones	607:634	circulating satiety hormones	607:634	Compared to paternal high fat/sucrose (HF/S), HP diet improved body composition and insulin sensitivity and improved circulating satiety hormones and cecal short-chain fatty acids compared to HF/S and control diet (P < .05).
34405464	3	53	theme	paternal	395:402	arg1	intake					407:412	paternal HP intake	395:412	paternal HP intake in Sprague Dawley rats	395:435	Here we show that paternal HP intake in Sprague Dawley rats programs protective metabolic outcomes in offspring.
34405464	2	54	theme	protein	278:284	arg1	diet					291:294	a pre-conception paternal high protein (HP) diet	247:294	a pre-conception paternal high protein (HP) diet	247:294	However, the contribution of a pre-conception paternal high protein (HP) diet to offspring metabolism, gut microbiota, and epigenetic changes remains unclear.
34405464	5	55	from	diversity	820:828	arg1	fathers					957:963	HP fathers	954:963	HP fathers	954:963	Further, using 16S rRNA gene sequencing to assess gut microbial composition, we observed increased alpha diversity, distinct bacterial clustering, and increased abundance of Bifidobacterium, Akkermansia, Bacteroides, and Marvinbryantia in HP fathers and/or male and female adult offspring.
34405464	5	55	from	diversity	820:828	arg1	offspring					994:1002	male and female adult offspring	972:1002	male and female adult offspring	972:1002	Further, using 16S rRNA gene sequencing to assess gut microbial composition, we observed increased alpha diversity, distinct bacterial clustering, and increased abundance of Bifidobacterium, Akkermansia, Bacteroides, and Marvinbryantia in HP fathers and/or male and female adult offspring.
34405464	6	56	theme	epigenetic	1012:1021	arg1	level					1023:1027	the epigenetic level	1008:1027	the epigenetic level	1008:1027	At the epigenetic level, DNMT1and 3b expression was altered intergenerationally.
34405464	4	57	theme	high	511:514	arg1	HF/S					529:532	HF/S	529:532	HF/S	529:532	Compared to paternal high fat/sucrose (HF/S), HP diet improved body composition and insulin sensitivity and improved circulating satiety hormones and cecal short-chain fatty acids compared to HF/S and control diet (P < .05).
34405464	4	57	theme	high	511:514	arg1	fat/sucrose					516:526	paternal high fat/sucrose	502:526	paternal high fat/sucrose (HF/S)	502:533	Compared to paternal high fat/sucrose (HF/S), HP diet improved body composition and insulin sensitivity and improved circulating satiety hormones and cecal short-chain fatty acids compared to HF/S and control diet (P < .05).
34405464	2	58	theme	high	273:276	arg1	diet					291:294	a pre-conception paternal high protein (HP) diet	247:294	a pre-conception paternal high protein (HP) diet	247:294	However, the contribution of a pre-conception paternal high protein (HP) diet to offspring metabolism, gut microbiota, and epigenetic changes remains unclear.
34405464	2	59	theme	gut	321:323	arg1	microbiota					325:334	gut microbiota	321:334	gut microbiota	321:334	However, the contribution of a pre-conception paternal high protein (HP) diet to offspring metabolism, gut microbiota, and epigenetic changes remains unclear.
34405464	5	60	theme	rRNA	734:737	arg1	sequencing					744:753	16S rRNA gene sequencing	730:753	16S rRNA gene sequencing	730:753	Further, using 16S rRNA gene sequencing to assess gut microbial composition, we observed increased alpha diversity, distinct bacterial clustering, and increased abundance of Bifidobacterium, Akkermansia, Bacteroides, and Marvinbryantia in HP fathers and/or male and female adult offspring.
34405464	4	61	theme	cecal	640:644	arg1	acids					664:668	cecal short-chain fatty acids	640:668	cecal short-chain fatty acids	640:668	Compared to paternal high fat/sucrose (HF/S), HP diet improved body composition and insulin sensitivity and improved circulating satiety hormones and cecal short-chain fatty acids compared to HF/S and control diet (P < .05).
34405464	2	62	theme	paternal	264:271	arg1	diet					291:294	a pre-conception paternal high protein (HP) diet	247:294	a pre-conception paternal high protein (HP) diet	247:294	However, the contribution of a pre-conception paternal high protein (HP) diet to offspring metabolism, gut microbiota, and epigenetic changes remains unclear.
34405464	5	63	theme	alpha	814:818	arg1	diversity					820:828	increased alpha diversity	804:828	increased alpha diversity	804:828	Further, using 16S rRNA gene sequencing to assess gut microbial composition, we observed increased alpha diversity, distinct bacterial clustering, and increased abundance of Bifidobacterium, Akkermansia, Bacteroides, and Marvinbryantia in HP fathers and/or male and female adult offspring.
34405464	1	64	theme	Mounting	137:144	arg1	evidence					146:153	Mounting evidence	137:153	Mounting evidence	137:153	Mounting evidence demonstrates that paternal diet programs offspring metabolism.
34405464	4	65	theme	satiety	619:625	arg1	hormones					627:634	circulating satiety hormones	607:634	circulating satiety hormones	607:634	Compared to paternal high fat/sucrose (HF/S), HP diet improved body composition and insulin sensitivity and improved circulating satiety hormones and cecal short-chain fatty acids compared to HF/S and control diet (P < .05).
34405464	0	66	theme	insulin	55:61	arg1	sensitivity					63:73	insulin sensitivity	55:73	insulin sensitivity	55:73	Paternal high protein diet modulates body composition, insulin sensitivity, epigenetics, and gut microbiota intergenerationally in rats.
34405464	4	67	theme	paternal	502:509	arg1	HF/S					529:532	HF/S	529:532	HF/S	529:532	Compared to paternal high fat/sucrose (HF/S), HP diet improved body composition and insulin sensitivity and improved circulating satiety hormones and cecal short-chain fatty acids compared to HF/S and control diet (P < .05).
34405464	4	67	theme	paternal	502:509	arg1	fat/sucrose					516:526	paternal high fat/sucrose	502:526	paternal high fat/sucrose (HF/S)	502:533	Compared to paternal high fat/sucrose (HF/S), HP diet improved body composition and insulin sensitivity and improved circulating satiety hormones and cecal short-chain fatty acids compared to HF/S and control diet (P < .05).
34405464	2	68	theme	pre-conception	249:262	arg1	diet					291:294	a pre-conception paternal high protein (HP) diet	247:294	a pre-conception paternal high protein (HP) diet	247:294	However, the contribution of a pre-conception paternal high protein (HP) diet to offspring metabolism, gut microbiota, and epigenetic changes remains unclear.
34405464	7	69	theme	composition	1156:1166	arg1	diet					1119:1122	paternal HP diet	1107:1122	paternal HP diet	1107:1122	Our study identifies paternal HP diet as a modulator of gut microbial composition, epigenetic markers, and metabolic function intergenerationally.
34405464	7	69	theme	composition	1156:1166	arg1	modulator					1129:1137	a modulator	1127:1137	a modulator of gut microbial composition, epigenetic markers, and metabolic function intergenerationally	1127:1230	Our study identifies paternal HP diet as a modulator of gut microbial composition, epigenetic markers, and metabolic function intergenerationally.
34405464	0	70	from	intergenerationally	108:126	arg1	rats					131:134	rats	131:134	rats	131:134	Paternal high protein diet modulates body composition, insulin sensitivity, epigenetics, and gut microbiota intergenerationally in rats.
33268034	6	0	theme	correlation	1052:1062	arg1	2DCOS					1083:1087	2DCOS	1083:1087	2DCOS	1083:1087	2D correlation spectral analysis (2DCOS) reveals cellulose and pectin begins changes followed by amide of proteins due to nitrogen treatment in P. peruviana samples.
33268034	6	0	theme	correlation	1052:1062	arg1	analysis					1073:1080	2D correlation spectral analysis	1049:1080	2D correlation spectral analysis (2DCOS)	1049:1088	2D correlation spectral analysis (2DCOS) reveals cellulose and pectin begins changes followed by amide of proteins due to nitrogen treatment in P. peruviana samples.
33268034	3	1	theme	Kg	673:674	arg1	ha-1					676:679	0, 200, 400, and 600 Kg ha-1	652:679	0, 200, 400, and 600 Kg ha-1	652:679	Both species were grown under different doses of nitrogen (0, 200, 400, and 600 Kg ha-1) and leaf samples were evaluated using ATR-FTIR.
33268034	3	1	theme	Kg	673:674	arg1	doses					633:637	different doses	623:637	different doses of nitrogen (0, 200, 400, and 600 Kg ha-1)	623:680	Both species were grown under different doses of nitrogen (0, 200, 400, and 600 Kg ha-1) and leaf samples were evaluated using ATR-FTIR.
33268034	11	2	theme	physalis	1895:1902	arg1	species					1904:1910	physalis species	1895:1910	physalis species due to nitrogen doses, constituting a fast and precise measuring for the suitable management of this fertilization	1895:2025	Thus the use of 2DCOS coupled with chemometrics helps to identify changes in the composition of leaves of physalis species due to nitrogen doses, constituting a fast and precise measuring for the suitable management of this fertilization.
33268034	2	3	with	combination	397:407	arg1	chemometrics					414:425	chemometrics	414:425	chemometrics	414:425	In this sense, the study aimed to apply FTIR technique in combination with chemometrics and ROC analysis for the evaluation of changes in compositional of plant leaves of Physalis angulata and Physalis peruviana due to nitrogen fertilization treatments.
33268034	11	4	from	changes	1855:1861	arg1	composition					1870:1880	the composition	1866:1880	the composition of leaves of physalis species due to nitrogen doses, constituting a fast and precise measuring for the suitable management of this fertilization	1866:2025	Thus the use of 2DCOS coupled with chemometrics helps to identify changes in the composition of leaves of physalis species due to nitrogen doses, constituting a fast and precise measuring for the suitable management of this fertilization.
33268034	3	5	theme	leaf	686:689	arg1	samples					691:697	leaf samples	686:697	leaf samples	686:697	Both species were grown under different doses of nitrogen (0, 200, 400, and 600 Kg ha-1) and leaf samples were evaluated using ATR-FTIR.
33268034	10	6	theme	dissimilarity	1768:1780	arg1	scale					1782:1786	dissimilarity scale	1768:1786	dissimilarity scale	1768:1786	Hierarchical cluster analysis shows closed separation for a similar group on dissimilarity scale.
33268034	5	7	theme	band	839:842	arg1	effective					1008:1016	effective	1008:1016	effective	1008:1016	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment is more effective for a better quality of yield.
33268034	5	7	theme	band	839:842	arg1	area					844:847	The computed band area	826:847	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment	826:998	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment is more effective for a better quality of yield.
33268034	6	8	theme	peruviana	1196:1204	arg1	samples					1206:1212	P. peruviana samples	1193:1212	P. peruviana samples	1193:1212	2D correlation spectral analysis (2DCOS) reveals cellulose and pectin begins changes followed by amide of proteins due to nitrogen treatment in P. peruviana samples.
33268034	1	9	theme	composition	211:221	arg1	essential					242:250	essential	242:250	essential	242:250	The determination of the molecular composition of plant leaves is essential to assist in nutritional management, whether for cultivated or non-cultivated species.
33268034	1	9	theme	composition	211:221	arg1	determination					180:192	The determination	176:192	The determination of the molecular composition of plant leaves	176:237	The determination of the molecular composition of plant leaves is essential to assist in nutritional management, whether for cultivated or non-cultivated species.
33268034	2	10	theme	fertilization	567:579	arg1	treatments					581:590	nitrogen fertilization treatments	558:590	nitrogen fertilization treatments	558:590	In this sense, the study aimed to apply FTIR technique in combination with chemometrics and ROC analysis for the evaluation of changes in compositional of plant leaves of Physalis angulata and Physalis peruviana due to nitrogen fertilization treatments.
33268034	10	11	from	group	1759:1763	arg1	scale					1782:1786	dissimilarity scale	1768:1786	dissimilarity scale	1768:1786	Hierarchical cluster analysis shows closed separation for a similar group on dissimilarity scale.
33268034	9	12	theme	higher	1592:1597	arg1	value					1599:1603	a higher value	1590:1603	a higher value of area under the curve reflecting better reliability of the experiments carried out	1590:1688	Receiver operation characteristic analysis shows a higher value of area under the curve reflecting better reliability of the experiments carried out.
33268034	1	13	theme	plant	226:230	arg1	composition					211:221	the molecular composition	197:221	the molecular composition of plant leaves	197:237	The determination of the molecular composition of plant leaves is essential to assist in nutritional management, whether for cultivated or non-cultivated species.
33268034	6	14	theme	P.	1193:1194	arg1	samples					1206:1212	P. peruviana samples	1193:1212	P. peruviana samples	1193:1212	2D correlation spectral analysis (2DCOS) reveals cellulose and pectin begins changes followed by amide of proteins due to nitrogen treatment in P. peruviana samples.
33268034	0	15	theme	operation	140:148	arg1	characteristics					150:164	Receiver operation characteristics	131:164	Receiver operation characteristics	131:164	Nitrogen influenced biomolecular changes on Physalis L. species studied using 2DCOS spectral analysis coupled with chemometric and Receiver operation characteristics analysis.
33268034	5	16	theme	yield	1042:1046	arg1	quality					1031:1037	a better quality	1022:1037	a better quality of yield	1022:1046	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment is more effective for a better quality of yield.
33268034	3	17	theme	different	623:631	arg1	ha-1					676:679	0, 200, 400, and 600 Kg ha-1	652:679	0, 200, 400, and 600 Kg ha-1	652:679	Both species were grown under different doses of nitrogen (0, 200, 400, and 600 Kg ha-1) and leaf samples were evaluated using ATR-FTIR.
33268034	3	17	theme	different	623:631	arg1	doses					633:637	different doses	623:637	different doses of nitrogen (0, 200, 400, and 600 Kg ha-1)	623:680	Both species were grown under different doses of nitrogen (0, 200, 400, and 600 Kg ha-1) and leaf samples were evaluated using ATR-FTIR.
33268034	5	18	theme	esterification	902:915	arg1	oxalate					929:935	calcium oxalate	921:935	calcium oxalate	921:935	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment is more effective for a better quality of yield.
33268034	5	18	theme	esterification	902:915	arg1	degree					892:897	degree	892:897	degree of esterification	892:915	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment is more effective for a better quality of yield.
33268034	5	18	theme	esterification	902:915	arg1	lipid/carbohydrates					871:889	lipid/carbohydrates	871:889	lipid/carbohydrates	871:889	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment is more effective for a better quality of yield.
33268034	5	18	theme	esterification	902:915	arg1	lipid/amide					858:868	lipid/amide	858:868	lipid/amide	858:868	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment is more effective for a better quality of yield.
33268034	6	19	theme	proteins	1155:1162	arg1	amide					1146:1150	amide	1146:1150	amide of proteins	1146:1162	2D correlation spectral analysis (2DCOS) reveals cellulose and pectin begins changes followed by amide of proteins due to nitrogen treatment in P. peruviana samples.
33268034	8	20	dep	show	1388:1391	arg1	predominant					1491:1501	predominant	1491:1501	predominant	1491:1501	The obtained principle component analysis plot and loading values show the Physalis species samples distinctly separated from control with protein and carbohydrates are predominant in influencing separation among them.
33268034	0	21	theme	2DCOS	78:82	arg1	analysis					93:100	2DCOS spectral analysis	78:100	2DCOS spectral analysis coupled with chemometric and Receiver operation characteristics analysis	78:173	Nitrogen influenced biomolecular changes on Physalis L. species studied using 2DCOS spectral analysis coupled with chemometric and Receiver operation characteristics analysis.
33268034	9	22	theme	Receiver	1541:1548	arg1	analysis					1575:1582	Receiver operation characteristic analysis	1541:1582	Receiver operation characteristic analysis	1541:1582	Receiver operation characteristic analysis shows a higher value of area under the curve reflecting better reliability of the experiments carried out.
33268034	2	23	theme	peruviana	541:549	arg1	plant					494:498	plant leaves	494:505	plant leaves of Physalis angulata and Physalis peruviana due to nitrogen fertilization treatments	494:590	In this sense, the study aimed to apply FTIR technique in combination with chemometrics and ROC analysis for the evaluation of changes in compositional of plant leaves of Physalis angulata and Physalis peruviana due to nitrogen fertilization treatments.
33268034	2	24	from	evaluation	452:461	arg1	compositional					477:489	compositional	477:489	compositional of plant leaves of Physalis angulata and Physalis peruviana due to nitrogen fertilization treatments	477:590	In this sense, the study aimed to apply FTIR technique in combination with chemometrics and ROC analysis for the evaluation of changes in compositional of plant leaves of Physalis angulata and Physalis peruviana due to nitrogen fertilization treatments.
33268034	5	25	dep	lipid/amide	858:868	arg1	the					854:856	the	854:856	the	854:856	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment is more effective for a better quality of yield.
33268034	5	25	dep	lipid/amide	858:868	arg1	shows					937:941	shows	937:941	shows nitrogen fertilization due to 400 Kg ha-1 of N treatment	937:998	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment is more effective for a better quality of yield.
33268034	9	26	theme	characteristic	1560:1573	arg1	analysis					1575:1582	Receiver operation characteristic analysis	1541:1582	Receiver operation characteristic analysis	1541:1582	Receiver operation characteristic analysis shows a higher value of area under the curve reflecting better reliability of the experiments carried out.
33268034	2	27	from	changes	466:472	arg1	compositional					477:489	compositional	477:489	compositional of plant leaves of Physalis angulata and Physalis peruviana due to nitrogen fertilization treatments	477:590	In this sense, the study aimed to apply FTIR technique in combination with chemometrics and ROC analysis for the evaluation of changes in compositional of plant leaves of Physalis angulata and Physalis peruviana due to nitrogen fertilization treatments.
33268034	2	28	theme	ROC	431:433	arg1	analysis					435:442	ROC analysis	431:442	ROC analysis	431:442	In this sense, the study aimed to apply FTIR technique in combination with chemometrics and ROC analysis for the evaluation of changes in compositional of plant leaves of Physalis angulata and Physalis peruviana due to nitrogen fertilization treatments.
33268034	8	29	theme	principle	1335:1343	arg1	analysis					1355:1362	principle component analysis	1335:1362	principle component analysis plot	1335:1367	The obtained principle component analysis plot and loading values show the Physalis species samples distinctly separated from control with protein and carbohydrates are predominant in influencing separation among them.
33268034	2	30	from	compositional	477:489	arg1	evaluation					452:461	the evaluation	448:461	the evaluation of changes in compositional of plant leaves of Physalis angulata and Physalis peruviana due to nitrogen fertilization treatments	448:590	In this sense, the study aimed to apply FTIR technique in combination with chemometrics and ROC analysis for the evaluation of changes in compositional of plant leaves of Physalis angulata and Physalis peruviana due to nitrogen fertilization treatments.
33268034	8	31	theme	analysis	1355:1362	arg1	plot					1364:1367	principle component analysis plot	1335:1367	principle component analysis plot	1335:1367	The obtained principle component analysis plot and loading values show the Physalis species samples distinctly separated from control with protein and carbohydrates are predominant in influencing separation among them.
33268034	5	32	theme	calcium	921:927	arg1	oxalate					929:935	calcium oxalate	921:935	calcium oxalate	921:935	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment is more effective for a better quality of yield.
33268034	9	33	theme	area	1608:1611	arg1	value					1599:1603	a higher value	1590:1603	a higher value of area under the curve reflecting better reliability of the experiments carried out	1590:1688	Receiver operation characteristic analysis shows a higher value of area under the curve reflecting better reliability of the experiments carried out.
33268034	0	34	theme	biomolecular	20:31	arg1	changes					33:39	biomolecular changes	20:39	biomolecular changes on Physalis L. species studied using 2DCOS spectral analysis coupled with chemometric and Receiver operation characteristics analysis	20:173	Nitrogen influenced biomolecular changes on Physalis L. species studied using 2DCOS spectral analysis coupled with chemometric and Receiver operation characteristics analysis.
33268034	10	35	theme	Hierarchical	1691:1702	arg1	analysis					1712:1719	Hierarchical cluster analysis	1691:1719	Hierarchical cluster analysis	1691:1719	Hierarchical cluster analysis shows closed separation for a similar group on dissimilarity scale.
33268034	5	36	theme	treatment	990:998	arg1	ha-1					980:983	400 Kg ha-1	973:983	400 Kg ha-1 of N treatment	973:998	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment is more effective for a better quality of yield.
33268034	0	37	theme	L.	53:54	arg1	species					56:62	Physalis L. species	44:62	Physalis L. species studied using 2DCOS spectral analysis coupled with chemometric and Receiver operation characteristics analysis	44:173	Nitrogen influenced biomolecular changes on Physalis L. species studied using 2DCOS spectral analysis coupled with chemometric and Receiver operation characteristics analysis.
33268034	2	38	dep	plant	494:498	arg1	leaves					500:505	leaves	500:505	leaves	500:505	In this sense, the study aimed to apply FTIR technique in combination with chemometrics and ROC analysis for the evaluation of changes in compositional of plant leaves of Physalis angulata and Physalis peruviana due to nitrogen fertilization treatments.
33268034	8	39	theme	species	1406:1412	arg1	samples					1414:1420	the Physalis species samples	1393:1420	the Physalis species samples distinctly separated from control with protein and carbohydrates	1393:1485	The obtained principle component analysis plot and loading values show the Physalis species samples distinctly separated from control with protein and carbohydrates are predominant in influencing separation among them.
33268034	10	40	theme	closed	1727:1732	arg1	separation					1734:1743	closed separation	1727:1743	closed separation	1727:1743	Hierarchical cluster analysis shows closed separation for a similar group on dissimilarity scale.
33268034	5	41	theme	Kg	977:978	arg1	ha-1					980:983	400 Kg ha-1	973:983	400 Kg ha-1 of N treatment	973:998	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment is more effective for a better quality of yield.
33268034	4	42	theme	species	779:785	arg1	spectra					763:769	the spectra	759:769	the spectra of both species	759:785	Our results demonstrate that the spectra of both species were influenced by the nitrogen doses.
33268034	2	43	theme	FTIR	379:382	arg1	technique					384:392	FTIR technique	379:392	FTIR technique in combination with chemometrics and ROC analysis	379:442	In this sense, the study aimed to apply FTIR technique in combination with chemometrics and ROC analysis for the evaluation of changes in compositional of plant leaves of Physalis angulata and Physalis peruviana due to nitrogen fertilization treatments.
33268034	6	44	theme	spectral	1064:1071	arg1	2DCOS					1083:1087	2DCOS	1083:1087	2DCOS	1083:1087	2D correlation spectral analysis (2DCOS) reveals cellulose and pectin begins changes followed by amide of proteins due to nitrogen treatment in P. peruviana samples.
33268034	6	44	theme	spectral	1064:1071	arg1	analysis					1073:1080	2D correlation spectral analysis	1049:1080	2D correlation spectral analysis (2DCOS)	1049:1088	2D correlation spectral analysis (2DCOS) reveals cellulose and pectin begins changes followed by amide of proteins due to nitrogen treatment in P. peruviana samples.
33268034	10	45	theme	similar	1751:1757	arg1	group					1759:1763	a similar group	1749:1763	a similar group on dissimilarity scale	1749:1786	Hierarchical cluster analysis shows closed separation for a similar group on dissimilarity scale.
33268034	1	46	theme	nutritional	265:275	arg1	management					277:286	nutritional management	265:286	nutritional management	265:286	The determination of the molecular composition of plant leaves is essential to assist in nutritional management, whether for cultivated or non-cultivated species.
33268034	9	47	theme	experiments	1666:1676	arg1	reliability					1647:1657	better reliability	1640:1657	better reliability of the experiments carried out	1640:1688	Receiver operation characteristic analysis shows a higher value of area under the curve reflecting better reliability of the experiments carried out.
33268034	6	48	theme	2D	1049:1050	arg1	2DCOS					1083:1087	2DCOS	1083:1087	2DCOS	1083:1087	2D correlation spectral analysis (2DCOS) reveals cellulose and pectin begins changes followed by amide of proteins due to nitrogen treatment in P. peruviana samples.
33268034	6	48	theme	2D	1049:1050	arg1	analysis					1073:1080	2D correlation spectral analysis	1049:1080	2D correlation spectral analysis (2DCOS)	1049:1088	2D correlation spectral analysis (2DCOS) reveals cellulose and pectin begins changes followed by amide of proteins due to nitrogen treatment in P. peruviana samples.
33268034	11	49	theme	fertilization	2013:2025	arg1	management					1994:2003	the suitable management	1981:2003	the suitable management of this fertilization	1981:2025	Thus the use of 2DCOS coupled with chemometrics helps to identify changes in the composition of leaves of physalis species due to nitrogen doses, constituting a fast and precise measuring for the suitable management of this fertilization.
33268034	5	50	theme	computed	830:837	arg1	effective					1008:1016	effective	1008:1016	effective	1008:1016	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment is more effective for a better quality of yield.
33268034	5	50	theme	computed	830:837	arg1	area					844:847	The computed band area	826:847	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment	826:998	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment is more effective for a better quality of yield.
33268034	2	51	with	analysis	435:442	arg1	chemometrics					414:425	chemometrics	414:425	chemometrics	414:425	In this sense, the study aimed to apply FTIR technique in combination with chemometrics and ROC analysis for the evaluation of changes in compositional of plant leaves of Physalis angulata and Physalis peruviana due to nitrogen fertilization treatments.
33268034	4	52	theme	nitrogen	810:817	arg1	doses					819:823	the nitrogen doses	806:823	the nitrogen doses	806:823	Our results demonstrate that the spectra of both species were influenced by the nitrogen doses.
33268034	6	53	dep	cellulose	1098:1106	arg1	begins					1119:1124	begins	1119:1124	begins changes followed by amide of proteins	1119:1162	2D correlation spectral analysis (2DCOS) reveals cellulose and pectin begins changes followed by amide of proteins due to nitrogen treatment in P. peruviana samples.
33268034	1	54	theme	molecular	201:209	arg1	composition					211:221	the molecular composition	197:221	the molecular composition of plant leaves	197:237	The determination of the molecular composition of plant leaves is essential to assist in nutritional management, whether for cultivated or non-cultivated species.
33268034	0	55	theme	Receiver	131:138	arg1	characteristics					150:164	Receiver operation characteristics	131:164	Receiver operation characteristics	131:164	Nitrogen influenced biomolecular changes on Physalis L. species studied using 2DCOS spectral analysis coupled with chemometric and Receiver operation characteristics analysis.
33268034	5	56	theme	nitrogen	943:950	arg1	fertilization					952:964	nitrogen fertilization	943:964	nitrogen fertilization	943:964	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment is more effective for a better quality of yield.
33268034	2	57	theme	nitrogen	558:565	arg1	treatments					581:590	nitrogen fertilization treatments	558:590	nitrogen fertilization treatments	558:590	In this sense, the study aimed to apply FTIR technique in combination with chemometrics and ROC analysis for the evaluation of changes in compositional of plant leaves of Physalis angulata and Physalis peruviana due to nitrogen fertilization treatments.
33268034	6	58	theme	nitrogen	1171:1178	arg1	treatment					1180:1188	nitrogen treatment	1171:1188	nitrogen treatment in P. peruviana samples	1171:1212	2D correlation spectral analysis (2DCOS) reveals cellulose and pectin begins changes followed by amide of proteins due to nitrogen treatment in P. peruviana samples.
33268034	6	59	from	treatment	1180:1188	arg1	samples					1206:1212	P. peruviana samples	1193:1212	P. peruviana samples	1193:1212	2D correlation spectral analysis (2DCOS) reveals cellulose and pectin begins changes followed by amide of proteins due to nitrogen treatment in P. peruviana samples.
33268034	3	60	theme	nitrogen	642:649	arg1	ha-1					676:679	0, 200, 400, and 600 Kg ha-1	652:679	0, 200, 400, and 600 Kg ha-1	652:679	Both species were grown under different doses of nitrogen (0, 200, 400, and 600 Kg ha-1) and leaf samples were evaluated using ATR-FTIR.
33268034	3	60	theme	nitrogen	642:649	arg1	doses					633:637	different doses	623:637	different doses of nitrogen (0, 200, 400, and 600 Kg ha-1)	623:680	Both species were grown under different doses of nitrogen (0, 200, 400, and 600 Kg ha-1) and leaf samples were evaluated using ATR-FTIR.
33268034	9	61	theme	operation	1550:1558	arg1	analysis					1575:1582	Receiver operation characteristic analysis	1541:1582	Receiver operation characteristic analysis	1541:1582	Receiver operation characteristic analysis shows a higher value of area under the curve reflecting better reliability of the experiments carried out.
33268034	5	62	theme	better	1024:1029	arg1	quality					1031:1037	a better quality	1022:1037	a better quality of yield	1022:1046	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment is more effective for a better quality of yield.
33268034	11	63	theme	suitable	1985:1992	arg1	management					1994:2003	the suitable management	1981:2003	the suitable management of this fertilization	1981:2025	Thus the use of 2DCOS coupled with chemometrics helps to identify changes in the composition of leaves of physalis species due to nitrogen doses, constituting a fast and precise measuring for the suitable management of this fertilization.
33268034	0	64	from	changes	33:39	arg1	species					56:62	Physalis L. species	44:62	Physalis L. species studied using 2DCOS spectral analysis coupled with chemometric and Receiver operation characteristics analysis	44:173	Nitrogen influenced biomolecular changes on Physalis L. species studied using 2DCOS spectral analysis coupled with chemometric and Receiver operation characteristics analysis.
33268034	0	65	theme	spectral	84:91	arg1	analysis					93:100	2DCOS spectral analysis	78:100	2DCOS spectral analysis coupled with chemometric and Receiver operation characteristics analysis	78:173	Nitrogen influenced biomolecular changes on Physalis L. species studied using 2DCOS spectral analysis coupled with chemometric and Receiver operation characteristics analysis.
33268034	2	66	theme	plant	494:498	arg1	compositional					477:489	compositional	477:489	compositional of plant leaves of Physalis angulata and Physalis peruviana due to nitrogen fertilization treatments	477:590	In this sense, the study aimed to apply FTIR technique in combination with chemometrics and ROC analysis for the evaluation of changes in compositional of plant leaves of Physalis angulata and Physalis peruviana due to nitrogen fertilization treatments.
33268034	11	67	theme	2DCOS	1805:1809	arg1	use					1798:1800	the use	1794:1800	the use of 2DCOS coupled with chemometrics	1794:1835	Thus the use of 2DCOS coupled with chemometrics helps to identify changes in the composition of leaves of physalis species due to nitrogen doses, constituting a fast and precise measuring for the suitable management of this fertilization.
33268034	9	68	theme	better	1640:1645	arg1	reliability					1647:1657	better reliability	1640:1657	better reliability of the experiments carried out	1640:1688	Receiver operation characteristic analysis shows a higher value of area under the curve reflecting better reliability of the experiments carried out.
33268034	5	69	from	lipid/carbohydrates	871:889	arg1	effective					1008:1016	effective	1008:1016	effective	1008:1016	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment is more effective for a better quality of yield.
33268034	5	69	from	lipid/carbohydrates	871:889	arg1	area					844:847	The computed band area	826:847	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment	826:998	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment is more effective for a better quality of yield.
33268034	0	70	dep	chemometric	115:125	arg1	analysis					166:173	analysis	166:173	analysis	166:173	Nitrogen influenced biomolecular changes on Physalis L. species studied using 2DCOS spectral analysis coupled with chemometric and Receiver operation characteristics analysis.
33268034	7	71	dep	angulata	1222:1229	arg1	plants					1231:1236	plants	1231:1236	The P. angulata plants	1215:1236	The P. angulata plants shows hemicellulose changes predominating followed by proteins and polysaccharides.
33268034	5	72	from	lipid/amide	858:868	arg1	effective					1008:1016	effective	1008:1016	effective	1008:1016	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment is more effective for a better quality of yield.
33268034	5	72	from	lipid/amide	858:868	arg1	area					844:847	The computed band area	826:847	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment	826:998	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment is more effective for a better quality of yield.
33268034	2	73	theme	angulata	519:526	arg1	plant					494:498	plant leaves	494:505	plant leaves of Physalis angulata and Physalis peruviana due to nitrogen fertilization treatments	494:590	In this sense, the study aimed to apply FTIR technique in combination with chemometrics and ROC analysis for the evaluation of changes in compositional of plant leaves of Physalis angulata and Physalis peruviana due to nitrogen fertilization treatments.
33268034	8	74	theme	obtained	1326:1333	arg1	values					1381:1386	The obtained principle component analysis plot and loading values	1322:1386	The obtained principle component analysis plot and loading values	1322:1386	The obtained principle component analysis plot and loading values show the Physalis species samples distinctly separated from control with protein and carbohydrates are predominant in influencing separation among them.
33268034	8	75	theme	component	1345:1353	arg1	analysis					1355:1362	principle component analysis	1335:1362	principle component analysis plot	1335:1367	The obtained principle component analysis plot and loading values show the Physalis species samples distinctly separated from control with protein and carbohydrates are predominant in influencing separation among them.
33268034	11	76	theme	nitrogen	1919:1926	arg1	doses					1928:1932	nitrogen doses	1919:1932	nitrogen doses	1919:1932	Thus the use of 2DCOS coupled with chemometrics helps to identify changes in the composition of leaves of physalis species due to nitrogen doses, constituting a fast and precise measuring for the suitable management of this fertilization.
33268034	1	77	theme	cultivated	301:310	arg1	species					330:336	cultivated or non-cultivated species	301:336	cultivated or non-cultivated species	301:336	The determination of the molecular composition of plant leaves is essential to assist in nutritional management, whether for cultivated or non-cultivated species.
33268034	5	78	from	degree	892:897	arg1	effective					1008:1016	effective	1008:1016	effective	1008:1016	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment is more effective for a better quality of yield.
33268034	5	78	from	degree	892:897	arg1	area					844:847	The computed band area	826:847	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment	826:998	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment is more effective for a better quality of yield.
33268034	8	79	theme	plot	1364:1367	arg1	values					1381:1386	The obtained principle component analysis plot and loading values	1322:1386	The obtained principle component analysis plot and loading values	1322:1386	The obtained principle component analysis plot and loading values show the Physalis species samples distinctly separated from control with protein and carbohydrates are predominant in influencing separation among them.
33268034	10	80	theme	cluster	1704:1710	arg1	analysis					1712:1719	Hierarchical cluster analysis	1691:1719	Hierarchical cluster analysis	1691:1719	Hierarchical cluster analysis shows closed separation for a similar group on dissimilarity scale.
33268034	0	81	theme	Physalis	44:51	arg1	species					56:62	Physalis L. species	44:62	Physalis L. species studied using 2DCOS spectral analysis coupled with chemometric and Receiver operation characteristics analysis	44:173	Nitrogen influenced biomolecular changes on Physalis L. species studied using 2DCOS spectral analysis coupled with chemometric and Receiver operation characteristics analysis.
33268034	1	82	theme	non-cultivated	315:328	arg1	species					330:336	cultivated or non-cultivated species	301:336	cultivated or non-cultivated species	301:336	The determination of the molecular composition of plant leaves is essential to assist in nutritional management, whether for cultivated or non-cultivated species.
33268034	5	83	theme	N	988:988	arg1	treatment					990:998	N treatment	988:998	N treatment	988:998	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment is more effective for a better quality of yield.
33268034	7	84	theme	hemicellulose	1244:1256	arg1	changes					1258:1264	hemicellulose changes	1244:1264	hemicellulose changes predominating followed by proteins and polysaccharides	1244:1319	The P. angulata plants shows hemicellulose changes predominating followed by proteins and polysaccharides.
33268034	8	85	theme	loading	1373:1379	arg1	values					1381:1386	The obtained principle component analysis plot and loading values	1322:1386	The obtained principle component analysis plot and loading values	1322:1386	The obtained principle component analysis plot and loading values show the Physalis species samples distinctly separated from control with protein and carbohydrates are predominant in influencing separation among them.
33268034	2	86	from	technique	384:392	arg1	combination					397:407	combination	397:407	combination with chemometrics	397:425	In this sense, the study aimed to apply FTIR technique in combination with chemometrics and ROC analysis for the evaluation of changes in compositional of plant leaves of Physalis angulata and Physalis peruviana due to nitrogen fertilization treatments.
33268034	2	86	from	technique	384:392	arg1	analysis					435:442	ROC analysis	431:442	ROC analysis	431:442	In this sense, the study aimed to apply FTIR technique in combination with chemometrics and ROC analysis for the evaluation of changes in compositional of plant leaves of Physalis angulata and Physalis peruviana due to nitrogen fertilization treatments.
33268034	2	87	theme	changes	466:472	arg1	evaluation					452:461	the evaluation	448:461	the evaluation of changes in compositional of plant leaves of Physalis angulata and Physalis peruviana due to nitrogen fertilization treatments	448:590	In this sense, the study aimed to apply FTIR technique in combination with chemometrics and ROC analysis for the evaluation of changes in compositional of plant leaves of Physalis angulata and Physalis peruviana due to nitrogen fertilization treatments.
33268034	11	88	theme	leaves	1885:1890	arg1	composition					1870:1880	the composition	1866:1880	the composition of leaves of physalis species due to nitrogen doses, constituting a fast and precise measuring for the suitable management of this fertilization	1866:2025	Thus the use of 2DCOS coupled with chemometrics helps to identify changes in the composition of leaves of physalis species due to nitrogen doses, constituting a fast and precise measuring for the suitable management of this fertilization.
33268034	5	89	from	oxalate	929:935	arg1	effective					1008:1016	effective	1008:1016	effective	1008:1016	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment is more effective for a better quality of yield.
33268034	5	89	from	oxalate	929:935	arg1	area					844:847	The computed band area	826:847	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment	826:998	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment is more effective for a better quality of yield.
33268034	5	90	theme	400	973:975	arg1	Kg					977:978	Kg	977:978	Kg	977:978	The computed band area from the lipid/amide, lipid/carbohydrates, degree of esterification and calcium oxalate shows nitrogen fertilization due to 400 Kg ha-1 of N treatment is more effective for a better quality of yield.
33268034	7	91	theme	P.	1219:1220	arg1	angulata					1222:1229	The P. angulata plants	1215:1236	The P. angulata plants	1215:1236	The P. angulata plants shows hemicellulose changes predominating followed by proteins and polysaccharides.
33268034	8	92	theme	Physalis	1397:1404	arg1	samples					1414:1420	the Physalis species samples	1393:1420	the Physalis species samples distinctly separated from control with protein and carbohydrates	1393:1485	The obtained principle component analysis plot and loading values show the Physalis species samples distinctly separated from control with protein and carbohydrates are predominant in influencing separation among them.
33268034	7	93	dep	predominating	1266:1278	arg1	followed					1280:1287	followed	1280:1287	followed by proteins and polysaccharides	1280:1319	The P. angulata plants shows hemicellulose changes predominating followed by proteins and polysaccharides.
33268034	1	94	dep	plant	226:230	arg1	leaves					232:237	leaves	232:237	leaves	232:237	The determination of the molecular composition of plant leaves is essential to assist in nutritional management, whether for cultivated or non-cultivated species.
33268034	11	95	theme	due	1912:1914	arg1	species					1904:1910	physalis species	1895:1910	physalis species due to nitrogen doses, constituting a fast and precise measuring for the suitable management of this fertilization	1895:2025	Thus the use of 2DCOS coupled with chemometrics helps to identify changes in the composition of leaves of physalis species due to nitrogen doses, constituting a fast and precise measuring for the suitable management of this fertilization.
34294349	5	0	theme	therapeutic	753:763	arg1	effects					765:771	excellent therapeutic effects	743:771	excellent therapeutic effects	743:771	In in vivo evaluations, the PLA EFMs presented excellent therapeutic effects by promoting structural and functional restoration of conjunctiva after transplant.
34294349	4	1	theme	bactericidal	674:685	arg1	effects					687:693	efficient bactericidal effects	664:693	efficient bactericidal effects	664:693	To prevent post-operative infections, the composite scaffolds were loaded with levofloxacin (LF), constantly exerting efficient bactericidal effects.
34294349	3	2	theme	SP	483:484	arg1	coating					486:492	the SP coating	479:492	the SP coating	479:492	The CNF coating improved the hydrophilicity and the SP coating proliferated conjunctival epithelial cells (CjECs).
34294349	5	3	theme	in	699:700	arg1	evaluations					707:717	in vivo evaluations	699:717	in vivo evaluations	699:717	In in vivo evaluations, the PLA EFMs presented excellent therapeutic effects by promoting structural and functional restoration of conjunctiva after transplant.
34294349	4	4	theme	post-operative	557:570	arg1	infections					572:581	post-operative infections	557:581	post-operative infections	557:581	To prevent post-operative infections, the composite scaffolds were loaded with levofloxacin (LF), constantly exerting efficient bactericidal effects.
34294349	2	5	theme	electrospun	318:328	arg1	surface					359:365	surface	359:365	surface	359:365	In this study, we have developed poly(lactic acid) (PLA) electrospun nanofibrous membranes (EFMs) surface coated by cellulose nanofibrils (CNF) and/or silk peptide (SP).
34294349	2	5	theme	electrospun	318:328	arg1	EFMs					353:356	EFMs	353:356	EFMs	353:356	In this study, we have developed poly(lactic acid) (PLA) electrospun nanofibrous membranes (EFMs) surface coated by cellulose nanofibrils (CNF) and/or silk peptide (SP).
34294349	2	5	theme	electrospun	318:328	arg1	membranes					342:350	poly(lactic acid) (PLA) electrospun nanofibrous membranes	294:350	poly(lactic acid) (PLA) electrospun nanofibrous membranes (EFMs) surface coated by cellulose nanofibrils (CNF) and/or silk peptide (SP)	294:428	In this study, we have developed poly(lactic acid) (PLA) electrospun nanofibrous membranes (EFMs) surface coated by cellulose nanofibrils (CNF) and/or silk peptide (SP).
34294349	5	6	theme	PLA	724:726	arg1	EFMs					728:731	the PLA EFMs	720:731	the PLA EFMs	720:731	In in vivo evaluations, the PLA EFMs presented excellent therapeutic effects by promoting structural and functional restoration of conjunctiva after transplant.
34294349	7	7	theme	antibiotics	1116:1126	arg1	post-surgery					1128:1139	antibiotics post-surgery	1116:1139	antibiotics post-surgery	1116:1139	It could be deduced that the potent bacterial inhibition feature could save troubles for patients by minimizing the application of antibiotics post-surgery.
34294349	2	8	theme	lactic	299:304	arg1	acid					306:309	lactic acid	299:309	lactic acid	299:309	In this study, we have developed poly(lactic acid) (PLA) electrospun nanofibrous membranes (EFMs) surface coated by cellulose nanofibrils (CNF) and/or silk peptide (SP).
34294349	2	8	theme	lactic	299:304	arg1	poly					294:297	poly	294:297	poly(lactic acid) (PLA) electrospun nanofibrous membranes (EFMs) surface coated by cellulose nanofibrils (CNF) and/or silk peptide (SP)	294:428	In this study, we have developed poly(lactic acid) (PLA) electrospun nanofibrous membranes (EFMs) surface coated by cellulose nanofibrils (CNF) and/or silk peptide (SP).
34294349	6	9	theme	antibiotics	901:911	arg1	administration					883:896	reduced topical administration	867:896	reduced topical administration of antibiotics	867:911	Even with reduced topical administration of antibiotics, the coloboma treated with LF loaded scaffolds presented no infections.
34294349	7	10	theme	post-surgery	1128:1139	arg1	application					1101:1111	the application	1097:1111	the application of antibiotics post-surgery	1097:1139	It could be deduced that the potent bacterial inhibition feature could save troubles for patients by minimizing the application of antibiotics post-surgery.
34294349	2	11	dep	coated	367:372	arg1	surface					359:365	surface	359:365	surface	359:365	In this study, we have developed poly(lactic acid) (PLA) electrospun nanofibrous membranes (EFMs) surface coated by cellulose nanofibrils (CNF) and/or silk peptide (SP).
34294349	2	11	dep	coated	367:372	arg1	membranes					342:350	poly(lactic acid) (PLA) electrospun nanofibrous membranes	294:350	poly(lactic acid) (PLA) electrospun nanofibrous membranes (EFMs) surface coated by cellulose nanofibrils (CNF) and/or silk peptide (SP)	294:428	In this study, we have developed poly(lactic acid) (PLA) electrospun nanofibrous membranes (EFMs) surface coated by cellulose nanofibrils (CNF) and/or silk peptide (SP).
34294349	1	12	theme	ocular	199:204	arg1	reconstruction					206:219	ocular reconstruction	199:219	ocular reconstruction with suitable conjunctival substitutes	199:258	The rehabilitation of visual acuity with severe conjunctival fibrosis depends on ocular reconstruction with suitable conjunctival substitutes.
34294349	5	13	theme	functional	801:810	arg1	restoration					812:822	structural and functional restoration	786:822	structural and functional restoration of conjunctiva	786:837	In in vivo evaluations, the PLA EFMs presented excellent therapeutic effects by promoting structural and functional restoration of conjunctiva after transplant.
34294349	5	14	dep	in	699:700	arg1	vivo					702:705	vivo	702:705	vivo	702:705	In in vivo evaluations, the PLA EFMs presented excellent therapeutic effects by promoting structural and functional restoration of conjunctiva after transplant.
34294349	0	15	dep	conjunctival	92:103	arg1	substitutes					105:115	substitutes	105:115	substitutes	105:115	Insight into levofloxacin loaded biocompatible electrospun scaffolds for their potential as conjunctival substitutes.
34294349	1	16	with	rehabilitation	122:135	arg1	fibrosis					179:186	severe conjunctival fibrosis	159:186	severe conjunctival fibrosis	159:186	The rehabilitation of visual acuity with severe conjunctival fibrosis depends on ocular reconstruction with suitable conjunctival substitutes.
34294349	2	17	theme	cellulose	377:385	arg1	CNF					400:402	CNF	400:402	CNF	400:402	In this study, we have developed poly(lactic acid) (PLA) electrospun nanofibrous membranes (EFMs) surface coated by cellulose nanofibrils (CNF) and/or silk peptide (SP).
34294349	2	17	theme	cellulose	377:385	arg1	nanofibrils					387:397	cellulose nanofibrils	377:397	cellulose nanofibrils (CNF)	377:403	In this study, we have developed poly(lactic acid) (PLA) electrospun nanofibrous membranes (EFMs) surface coated by cellulose nanofibrils (CNF) and/or silk peptide (SP).
34294349	5	18	theme	conjunctiva	827:837	arg1	restoration					812:822	structural and functional restoration	786:822	structural and functional restoration of conjunctiva	786:837	In in vivo evaluations, the PLA EFMs presented excellent therapeutic effects by promoting structural and functional restoration of conjunctiva after transplant.
34294349	3	19	theme	epithelial	520:529	arg1	CjECs					538:542	CjECs	538:542	CjECs	538:542	The CNF coating improved the hydrophilicity and the SP coating proliferated conjunctival epithelial cells (CjECs).
34294349	3	19	theme	epithelial	520:529	arg1	cells					531:535	conjunctival epithelial cells	507:535	conjunctival epithelial cells (CjECs)	507:543	The CNF coating improved the hydrophilicity and the SP coating proliferated conjunctival epithelial cells (CjECs).
34294349	6	20	dep	scaffolds	950:958	arg1	presented					960:968	presented	960:968	scaffolds presented no infections	950:982	Even with reduced topical administration of antibiotics, the coloboma treated with LF loaded scaffolds presented no infections.
34294349	2	21	theme	silk	412:415	arg1	SP					426:427	SP	426:427	SP	426:427	In this study, we have developed poly(lactic acid) (PLA) electrospun nanofibrous membranes (EFMs) surface coated by cellulose nanofibrils (CNF) and/or silk peptide (SP).
34294349	2	21	theme	silk	412:415	arg1	peptide					417:423	silk peptide	412:423	silk peptide (SP)	412:428	In this study, we have developed poly(lactic acid) (PLA) electrospun nanofibrous membranes (EFMs) surface coated by cellulose nanofibrils (CNF) and/or silk peptide (SP).
34294349	0	22	theme	electrospun	47:57	arg1	scaffolds					59:67	biocompatible electrospun scaffolds	33:67	biocompatible electrospun scaffolds	33:67	Insight into levofloxacin loaded biocompatible electrospun scaffolds for their potential as conjunctival substitutes.
34294349	6	23	theme	topical	875:881	arg1	administration					883:896	reduced topical administration	867:896	reduced topical administration of antibiotics	867:911	Even with reduced topical administration of antibiotics, the coloboma treated with LF loaded scaffolds presented no infections.
34294349	0	24	theme	biocompatible	33:45	arg1	scaffolds					59:67	biocompatible electrospun scaffolds	33:67	biocompatible electrospun scaffolds	33:67	Insight into levofloxacin loaded biocompatible electrospun scaffolds for their potential as conjunctival substitutes.
34294349	6	25	theme	reduced	867:873	arg1	administration					883:896	reduced topical administration	867:896	reduced topical administration of antibiotics	867:911	Even with reduced topical administration of antibiotics, the coloboma treated with LF loaded scaffolds presented no infections.
34294349	7	26	theme	inhibition	1031:1040	arg1	feature					1042:1048	the potent bacterial inhibition feature	1010:1048	the potent bacterial inhibition feature	1010:1048	It could be deduced that the potent bacterial inhibition feature could save troubles for patients by minimizing the application of antibiotics post-surgery.
34294349	3	27	theme	CNF	435:437	arg1	coating					439:445	The CNF coating	431:445	The CNF coating	431:445	The CNF coating improved the hydrophilicity and the SP coating proliferated conjunctival epithelial cells (CjECs).
34294349	5	28	theme	structural	786:795	arg1	restoration					812:822	structural and functional restoration	786:822	structural and functional restoration of conjunctiva	786:837	In in vivo evaluations, the PLA EFMs presented excellent therapeutic effects by promoting structural and functional restoration of conjunctiva after transplant.
34294349	1	29	with	reconstruction	206:219	arg1	conjunctival					235:246	conjunctival	235:246	conjunctival	235:246	The rehabilitation of visual acuity with severe conjunctival fibrosis depends on ocular reconstruction with suitable conjunctival substitutes.
34294349	8	30	theme	developed	1153:1161	arg1	EFMs					1167:1170	the developed PLA EFMs	1149:1170	the developed PLA EFMs loaded with LF	1149:1185	Hence, the developed PLA EFMs loaded with LF could be promising conjunctival substitutes.
34294349	8	30	theme	developed	1153:1161	arg1	substitutes					1219:1229	substitutes	1219:1229	substitutes	1219:1229	Hence, the developed PLA EFMs loaded with LF could be promising conjunctival substitutes.
34294349	8	30	theme	developed	1153:1161	arg1	conjunctival					1206:1217	conjunctival	1206:1217	conjunctival	1206:1217	Hence, the developed PLA EFMs loaded with LF could be promising conjunctival substitutes.
34294349	5	31	attach	presented	733:741	arg2	EFMs					728:731	the PLA EFMs	720:731	the PLA EFMs	720:731	In in vivo evaluations, the PLA EFMs presented excellent therapeutic effects by promoting structural and functional restoration of conjunctiva after transplant.
34294349	5	31	attach	presented	733:741	arg1	evaluations					707:717	in vivo evaluations	699:717	in vivo evaluations	699:717	In in vivo evaluations, the PLA EFMs presented excellent therapeutic effects by promoting structural and functional restoration of conjunctiva after transplant.
34294349	1	32	theme	conjunctival	166:177	arg1	fibrosis					179:186	severe conjunctival fibrosis	159:186	severe conjunctival fibrosis	159:186	The rehabilitation of visual acuity with severe conjunctival fibrosis depends on ocular reconstruction with suitable conjunctival substitutes.
34294349	7	33	theme	potent	1014:1019	arg1	feature					1042:1048	the potent bacterial inhibition feature	1010:1048	the potent bacterial inhibition feature	1010:1048	It could be deduced that the potent bacterial inhibition feature could save troubles for patients by minimizing the application of antibiotics post-surgery.
34294349	1	34	theme	visual	140:145	arg1	acuity					147:152	visual acuity	140:152	visual acuity	140:152	The rehabilitation of visual acuity with severe conjunctival fibrosis depends on ocular reconstruction with suitable conjunctival substitutes.
34294349	8	35	dep	conjunctival	1206:1217	arg1	substitutes					1219:1229	substitutes	1219:1229	substitutes	1219:1229	Hence, the developed PLA EFMs loaded with LF could be promising conjunctival substitutes.
34294349	8	35	dep	conjunctival	1206:1217	arg1	EFMs					1167:1170	the developed PLA EFMs	1149:1170	the developed PLA EFMs loaded with LF	1149:1185	Hence, the developed PLA EFMs loaded with LF could be promising conjunctival substitutes.
34294349	8	35	dep	conjunctival	1206:1217	arg1	conjunctival					1206:1217	conjunctival	1206:1217	conjunctival	1206:1217	Hence, the developed PLA EFMs loaded with LF could be promising conjunctival substitutes.
34294349	2	36	theme	poly	294:297	arg1	surface					359:365	surface	359:365	surface	359:365	In this study, we have developed poly(lactic acid) (PLA) electrospun nanofibrous membranes (EFMs) surface coated by cellulose nanofibrils (CNF) and/or silk peptide (SP).
34294349	2	36	theme	poly	294:297	arg1	EFMs					353:356	EFMs	353:356	EFMs	353:356	In this study, we have developed poly(lactic acid) (PLA) electrospun nanofibrous membranes (EFMs) surface coated by cellulose nanofibrils (CNF) and/or silk peptide (SP).
34294349	2	36	theme	poly	294:297	arg1	membranes					342:350	poly(lactic acid) (PLA) electrospun nanofibrous membranes	294:350	poly(lactic acid) (PLA) electrospun nanofibrous membranes (EFMs) surface coated by cellulose nanofibrils (CNF) and/or silk peptide (SP)	294:428	In this study, we have developed poly(lactic acid) (PLA) electrospun nanofibrous membranes (EFMs) surface coated by cellulose nanofibrils (CNF) and/or silk peptide (SP).
34294349	4	37	theme	efficient	664:672	arg1	effects					687:693	efficient bactericidal effects	664:693	efficient bactericidal effects	664:693	To prevent post-operative infections, the composite scaffolds were loaded with levofloxacin (LF), constantly exerting efficient bactericidal effects.
34294349	1	38	theme	acuity	147:152	arg1	rehabilitation					122:135	The rehabilitation	118:135	The rehabilitation of visual acuity with severe conjunctival fibrosis	118:186	The rehabilitation of visual acuity with severe conjunctival fibrosis depends on ocular reconstruction with suitable conjunctival substitutes.
34294349	7	39	theme	bacterial	1021:1029	arg1	feature					1042:1048	the potent bacterial inhibition feature	1010:1048	the potent bacterial inhibition feature	1010:1048	It could be deduced that the potent bacterial inhibition feature could save troubles for patients by minimizing the application of antibiotics post-surgery.
34294349	2	40	theme	nanofibrous	330:340	arg1	surface					359:365	surface	359:365	surface	359:365	In this study, we have developed poly(lactic acid) (PLA) electrospun nanofibrous membranes (EFMs) surface coated by cellulose nanofibrils (CNF) and/or silk peptide (SP).
34294349	2	40	theme	nanofibrous	330:340	arg1	EFMs					353:356	EFMs	353:356	EFMs	353:356	In this study, we have developed poly(lactic acid) (PLA) electrospun nanofibrous membranes (EFMs) surface coated by cellulose nanofibrils (CNF) and/or silk peptide (SP).
34294349	2	40	theme	nanofibrous	330:340	arg1	membranes					342:350	poly(lactic acid) (PLA) electrospun nanofibrous membranes	294:350	poly(lactic acid) (PLA) electrospun nanofibrous membranes (EFMs) surface coated by cellulose nanofibrils (CNF) and/or silk peptide (SP)	294:428	In this study, we have developed poly(lactic acid) (PLA) electrospun nanofibrous membranes (EFMs) surface coated by cellulose nanofibrils (CNF) and/or silk peptide (SP).
34294349	4	41	theme	composite	588:596	arg1	scaffolds					598:606	the composite scaffolds	584:606	the composite scaffolds	584:606	To prevent post-operative infections, the composite scaffolds were loaded with levofloxacin (LF), constantly exerting efficient bactericidal effects.
34294349	8	42	theme	PLA	1163:1165	arg1	EFMs					1167:1170	the developed PLA EFMs	1149:1170	the developed PLA EFMs loaded with LF	1149:1185	Hence, the developed PLA EFMs loaded with LF could be promising conjunctival substitutes.
34294349	8	42	theme	PLA	1163:1165	arg1	substitutes					1219:1229	substitutes	1219:1229	substitutes	1219:1229	Hence, the developed PLA EFMs loaded with LF could be promising conjunctival substitutes.
34294349	8	42	theme	PLA	1163:1165	arg1	conjunctival					1206:1217	conjunctival	1206:1217	conjunctival	1206:1217	Hence, the developed PLA EFMs loaded with LF could be promising conjunctival substitutes.
34294349	1	43	theme	severe	159:164	arg1	fibrosis					179:186	severe conjunctival fibrosis	159:186	severe conjunctival fibrosis	159:186	The rehabilitation of visual acuity with severe conjunctival fibrosis depends on ocular reconstruction with suitable conjunctival substitutes.
34294349	5	44	theme	excellent	743:751	arg1	effects					765:771	excellent therapeutic effects	743:771	excellent therapeutic effects	743:771	In in vivo evaluations, the PLA EFMs presented excellent therapeutic effects by promoting structural and functional restoration of conjunctiva after transplant.
34294349	1	45	dep	conjunctival	235:246	arg1	substitutes					248:258	substitutes	248:258	substitutes	248:258	The rehabilitation of visual acuity with severe conjunctival fibrosis depends on ocular reconstruction with suitable conjunctival substitutes.
34294349	3	46	theme	conjunctival	507:518	arg1	CjECs					538:542	CjECs	538:542	CjECs	538:542	The CNF coating improved the hydrophilicity and the SP coating proliferated conjunctival epithelial cells (CjECs).
34294349	3	46	theme	conjunctival	507:518	arg1	cells					531:535	conjunctival epithelial cells	507:535	conjunctival epithelial cells (CjECs)	507:543	The CNF coating improved the hydrophilicity and the SP coating proliferated conjunctival epithelial cells (CjECs).
34516367	2	0	theme	Acheta	110:115	arg1	domesticus					117:126	the cricket Acheta domesticus	98:126	the cricket Acheta domesticus	98:126	Strain F2AT, isolated from the cricket Acheta domesticus, was subjected to a polyphasic taxonomic characterization.
34516367	6	1	theme	similarity	441:450	arg1	values					452:457	The next highest similarity values	424:457	The next highest similarity values	424:457	The next highest similarity values were found to representatives of related genera (<93 %).
34516367	8	2	theme	74.29/74.43	742:752	arg1	%					771:771	74.29/74.43, 83.88 and 74.70 %	742:771	%	771:771	Average nucleotide identity values based on blast and MUMmer and average amino acid identity values between strain F2AT and E. moraniae QZS01T were 74.29/74.43, 83.88 and 74.70 %, respectively.
34516367	8	3	theme	identity	613:620	arg1	values					622:627	Average nucleotide identity values	594:627	Average nucleotide identity values based on blast and MUMmer and average amino acid identity values between strain F2AT and E. moraniae QZS01T	594:735	Average nucleotide identity values based on blast and MUMmer and average amino acid identity values between strain F2AT and E. moraniae QZS01T were 74.29/74.43, 83.88 and 74.70 %, respectively.
34516367	2	4	theme	cricket	102:108	arg1	domesticus					117:126	the cricket Acheta domesticus	98:126	the cricket Acheta domesticus	98:126	Strain F2AT, isolated from the cricket Acheta domesticus, was subjected to a polyphasic taxonomic characterization.
34516367	6	5	theme	highest	433:439	arg1	values					452:457	The next highest similarity values	424:457	The next highest similarity values	424:457	The next highest similarity values were found to representatives of related genera (<93 %).
34516367	12	6	theme	fatty	1082:1086	arg1	acids					1088:1092	Major fatty acids	1076:1092	Major fatty acids	1076:1092	Major fatty acids were C18 : 1 ω7c and C16 : 0 and the hydroxyl acids were C12 : 0 3-OH, C14 : 0 2-OH and C14 : 0 3-OH.
34516367	12	7	dep	C18 	1099:1102	arg1	3-OH					1190:1193	 0 3-OH	1187:1193	 0 3-OH	1187:1193	Major fatty acids were C18 : 1 ω7c and C16 : 0 and the hydroxyl acids were C12 : 0 3-OH, C14 : 0 2-OH and C14 : 0 3-OH.
34516367	5	8	theme	highest	364:370	arg1	similarity					372:381	highest similarity	364:381	highest similarity to Entomomonas moraniae QZS01T (96.4 %)	364:421	The strain's 16S rRNA gene sequence showed highest similarity to Entomomonas moraniae QZS01T (96.4 %).
34516367	12	9	theme	hydroxyl	1131:1138	arg1	acids					1140:1144	the hydroxyl acids	1127:1144	the hydroxyl acids	1127:1144	Major fatty acids were C18 : 1 ω7c and C16 : 0 and the hydroxyl acids were C12 : 0 3-OH, C14 : 0 2-OH and C14 : 0 3-OH.
34516367	12	9	theme	hydroxyl	1131:1138	arg1	C12 					1151:1154	C12 	1151:1154	C12 	1151:1154	Major fatty acids were C18 : 1 ω7c and C16 : 0 and the hydroxyl acids were C12 : 0 3-OH, C14 : 0 2-OH and C14 : 0 3-OH.
34516367	3	10	theme	strain	200:205	arg1	Cells					187:191	Cells	187:191	Cells of the strain	187:205	Cells of the strain were rod-shaped, Gram-stain-negative and catalase- and oxidase-positive.
34516367	1	11	theme	Acheta	52:57	arg1	domesticus					59:68	Acheta domesticus	52:68	Acheta domesticus	52:68	nov., isolated from Acheta domesticus.
34516367	10	12	located	detected	984:991	arg1	profile					866:872	the polar lipid profile	850:872	the polar lipid profile	850:872	In the polar lipid profile, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid were detected.
34516367	10	12	located	detected	984:991	arg2	phosphatidylglycerol					925:944	phosphatidylglycerol	925:944	phosphatidylglycerol	925:944	In the polar lipid profile, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid were detected.
34516367	10	12	located	detected	984:991	arg2	phosphatidylethanolamine					899:922	phosphatidylethanolamine	899:922	phosphatidylethanolamine	899:922	In the polar lipid profile, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid were detected.
34516367	10	12	located	detected	984:991	arg2	diphosphatidylglycerol					875:896	diphosphatidylglycerol	875:896	diphosphatidylglycerol	875:896	In the polar lipid profile, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid were detected.
34516367	10	12	located	detected	984:991	arg2	phospholipid					966:977	an unidentified phospholipid	950:977	an unidentified phospholipid	950:977	In the polar lipid profile, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid were detected.
34516367	9	13	contain	contained	821:829	arg1	system					800:805	The quinone system	788:805	The quinone system	788:805	The quinone system predominantly contained ubiquinone Q-8.
34516367	9	13	contain	contained	821:829	arg2	Q-8					842:844	ubiquinone Q-8	831:844	ubiquinone Q-8	831:844	The quinone system predominantly contained ubiquinone Q-8.
34516367	13	14	theme	diagnostic	1200:1209	arg1	acid					1219:1222	The diagnostic diamino acid	1196:1222	The diagnostic diamino acid of the peptidoglycan	1196:1243	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
34516367	13	14	theme	diagnostic	1200:1209	arg1	acid					1269:1272	meso-diaminopimelic acid	1249:1272	meso-diaminopimelic acid	1249:1272	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
34516367	13	15	theme	meso-diaminopimelic	1249:1267	arg1	acid					1219:1222	The diagnostic diamino acid	1196:1222	The diagnostic diamino acid of the peptidoglycan	1196:1243	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
34516367	13	15	theme	meso-diaminopimelic	1249:1267	arg1	acid					1269:1272	meso-diaminopimelic acid	1249:1272	meso-diaminopimelic acid	1249:1272	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
34516367	7	16	theme	36.4 mol	583:590	arg1	content					571:577	the G+C content	563:577	the G+C content	563:577	The genome size of strain F2AT was 3.2 Mbp and the G+C content was 36.4 mol%.
34516367	7	16	theme	36.4 mol	583:590	arg1	%					591:591	36.4 mol%	583:591	36.4 mol%	583:591	The genome size of strain F2AT was 3.2 Mbp and the G+C content was 36.4 mol%.
34516367	10	17	theme	lipid	860:864	arg1	profile					866:872	the polar lipid profile	850:872	the polar lipid profile	850:872	In the polar lipid profile, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid were detected.
34516367	7	18	theme	G+C	567:569	arg1	content					571:577	the G+C content	563:577	the G+C content	563:577	The genome size of strain F2AT was 3.2 Mbp and the G+C content was 36.4 mol%.
34516367	7	18	theme	G+C	567:569	arg1	%					591:591	36.4 mol%	583:591	36.4 mol%	583:591	The genome size of strain F2AT was 3.2 Mbp and the G+C content was 36.4 mol%.
34516367	10	19	theme	polar	854:858	arg1	profile					866:872	the polar lipid profile	850:872	the polar lipid profile	850:872	In the polar lipid profile, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid were detected.
34516367	8	20	theme	identity	678:685	arg1	values					687:692	blast and MUMmer and average amino acid identity values	638:692	blast and MUMmer and average amino acid identity values between strain F2AT and E. moraniae QZS01T	638:735	Average nucleotide identity values based on blast and MUMmer and average amino acid identity values between strain F2AT and E. moraniae QZS01T were 74.29/74.43, 83.88 and 74.70 %, respectively.
34516367	0	21	theme	asaccharolytica	12:26	arg1	sp					28:29	Entomomonas asaccharolytica sp	0:29	Entomomonas asaccharolytica sp.	0:30	Entomomonas asaccharolytica sp.
34516367	8	22	theme	MUMmer	648:653	arg1	values					687:692	blast and MUMmer and average amino acid identity values	638:692	blast and MUMmer and average amino acid identity values between strain F2AT and E. moraniae QZS01T	638:735	Average nucleotide identity values based on blast and MUMmer and average amino acid identity values between strain F2AT and E. moraniae QZS01T were 74.29/74.43, 83.88 and 74.70 %, respectively.
34516367	5	23	theme	16S	334:336	arg1	rRNA					338:341	strain's 16S rRNA	325:341	The strain's 16S rRNA gene sequence	321:355	The strain's 16S rRNA gene sequence showed highest similarity to Entomomonas moraniae QZS01T (96.4 %).
34516367	7	24	theme	F2AT	542:545	arg1	Mbp					555:557	3.2 Mbp	551:557	3.2 Mbp	551:557	The genome size of strain F2AT was 3.2 Mbp and the G+C content was 36.4 mol%.
34516367	7	24	theme	F2AT	542:545	arg1	size					527:530	The genome size	516:530	The genome size of strain F2AT	516:545	The genome size of strain F2AT was 3.2 Mbp and the G+C content was 36.4 mol%.
34516367	8	25	theme	blast	638:642	arg1	values					687:692	blast and MUMmer and average amino acid identity values	638:692	blast and MUMmer and average amino acid identity values between strain F2AT and E. moraniae QZS01T	638:735	Average nucleotide identity values based on blast and MUMmer and average amino acid identity values between strain F2AT and E. moraniae QZS01T were 74.29/74.43, 83.88 and 74.70 %, respectively.
34516367	14	26	from	moraniae	1374:1381	arg1	distinctness					1353:1364	its distinctness	1349:1364	its distinctness from E. moraniae	1349:1381	Due to its association with the only species of the genus Entomomonas but its distinctness from E. moraniae we here propose the novel species Entomomonas asaccharolytica sp.
34516367	0	27	theme	Entomomonas	0:10	arg1	sp					28:29	Entomomonas asaccharolytica sp	0:29	Entomomonas asaccharolytica sp.	0:30	Entomomonas asaccharolytica sp.
34516367	2	28	theme	Strain	71:76	arg1	F2AT					78:81	Strain F2AT	71:81	Strain F2AT	71:81	Strain F2AT, isolated from the cricket Acheta domesticus, was subjected to a polyphasic taxonomic characterization.
34516367	8	29	theme	Average	594:600	arg1	identity					613:620	Average nucleotide identity	594:620	Average nucleotide identity values based on blast and MUMmer and average amino acid identity values between strain F2AT and E. moraniae QZS01T	594:735	Average nucleotide identity values based on blast and MUMmer and average amino acid identity values between strain F2AT and E. moraniae QZS01T were 74.29/74.43, 83.88 and 74.70 %, respectively.
34516367	14	30	theme	only	1307:1310	arg1	species					1312:1318	the only species	1303:1318	the only species of the genus Entomomonas	1303:1343	Due to its association with the only species of the genus Entomomonas but its distinctness from E. moraniae we here propose the novel species Entomomonas asaccharolytica sp.
34516367	12	31	theme	Major	1076:1080	arg1	acids					1088:1092	Major fatty acids	1076:1092	Major fatty acids	1076:1092	Major fatty acids were C18 : 1 ω7c and C16 : 0 and the hydroxyl acids were C12 : 0 3-OH, C14 : 0 2-OH and C14 : 0 3-OH.
34516367	5	32	theme	rRNA	338:341	arg1	sequence					348:355	The strain's 16S rRNA gene sequence	321:355	The strain's 16S rRNA gene sequence	321:355	The strain's 16S rRNA gene sequence showed highest similarity to Entomomonas moraniae QZS01T (96.4 %).
34516367	6	33	theme	genera	500:505	arg1	representatives					473:487	representatives	473:487	representatives of related genera (<93 %)	473:513	The next highest similarity values were found to representatives of related genera (<93 %).
34516367	16	34	theme	=CCM	1460:1463	arg1	32211T					1475:1480	=CCM 9136T=LMG 32211T	1460:1480	=CCM 9136T=LMG 32211T	1460:1480	F2AT (=CCM 9136T=LMG 32211T).
34516367	16	34	theme	=CCM	1460:1463	arg1	F2AT					1454:1457	F2AT	1454:1457	F2AT (=CCM 9136T=LMG 32211T).	1454:1482	F2AT (=CCM 9136T=LMG 32211T).
34516367	10	35	theme	unidentified	953:964	arg1	phospholipid					966:977	an unidentified phospholipid	950:977	an unidentified phospholipid	950:977	In the polar lipid profile, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid were detected.
34516367	5	36	theme	gene	343:346	arg1	sequence					348:355	The strain's 16S rRNA gene sequence	321:355	The strain's 16S rRNA gene sequence	321:355	The strain's 16S rRNA gene sequence showed highest similarity to Entomomonas moraniae QZS01T (96.4 %).
34516367	6	37	theme	related	492:498	arg1	%					512:512	<93 %	508:512	<93 %	508:512	The next highest similarity values were found to representatives of related genera (<93 %).
34516367	6	37	theme	related	492:498	arg1	genera					500:505	related genera	492:505	related genera (<93 %)	492:513	The next highest similarity values were found to representatives of related genera (<93 %).
34516367	11	38	dep	compounds	1039:1047	arg1	spermidine					1064:1073	spermidine	1064:1073	spermidine	1064:1073	The polyamine pattern consisted of the major compounds putrescine and spermidine.
34516367	11	38	dep	compounds	1039:1047	arg1	putrescine					1049:1058	putrescine	1049:1058	putrescine	1049:1058	The polyamine pattern consisted of the major compounds putrescine and spermidine.
34516367	11	38	dep	compounds	1039:1047	arg1	compounds					1039:1047	the major compounds putrescine and spermidine	1029:1073	the major compounds putrescine and spermidine	1029:1073	The polyamine pattern consisted of the major compounds putrescine and spermidine.
34516367	7	39	theme	strain	535:540	arg1	F2AT					542:545	strain F2AT	535:545	strain F2AT	535:545	The genome size of strain F2AT was 3.2 Mbp and the G+C content was 36.4 mol%.
34516367	14	40	theme	Entomomonas	1333:1343	arg1	species					1312:1318	the only species	1303:1318	the only species of the genus Entomomonas	1303:1343	Due to its association with the only species of the genus Entomomonas but its distinctness from E. moraniae we here propose the novel species Entomomonas asaccharolytica sp.
34516367	12	41	theme	 0	1187:1188	arg1	3-OH					1190:1193	 0 3-OH	1187:1193	 0 3-OH	1187:1193	Major fatty acids were C18 : 1 ω7c and C16 : 0 and the hydroxyl acids were C12 : 0 3-OH, C14 : 0 2-OH and C14 : 0 3-OH.
34516367	8	42	theme	acid	673:676	arg1	identity					678:685	average amino acid identity	659:685	average amino acid identity	659:685	Average nucleotide identity values based on blast and MUMmer and average amino acid identity values between strain F2AT and E. moraniae QZS01T were 74.29/74.43, 83.88 and 74.70 %, respectively.
34516367	2	43	theme	taxonomic	159:167	arg1	characterization					169:184	a polyphasic taxonomic characterization	146:184	a polyphasic taxonomic characterization	146:184	Strain F2AT, isolated from the cricket Acheta domesticus, was subjected to a polyphasic taxonomic characterization.
34516367	7	44	theme	genome	520:525	arg1	Mbp					555:557	3.2 Mbp	551:557	3.2 Mbp	551:557	The genome size of strain F2AT was 3.2 Mbp and the G+C content was 36.4 mol%.
34516367	7	44	theme	genome	520:525	arg1	size					527:530	The genome size	516:530	The genome size of strain F2AT	516:545	The genome size of strain F2AT was 3.2 Mbp and the G+C content was 36.4 mol%.
34516367	14	45	theme	genus	1327:1331	arg1	Entomomonas					1333:1343	the genus Entomomonas	1323:1343	the genus Entomomonas	1323:1343	Due to its association with the only species of the genus Entomomonas but its distinctness from E. moraniae we here propose the novel species Entomomonas asaccharolytica sp.
34516367	14	46	theme	asaccharolytica	1429:1443	arg1	sp					1445:1446	Entomomonas asaccharolytica sp	1417:1446	the novel species Entomomonas asaccharolytica sp	1399:1446	Due to its association with the only species of the genus Entomomonas but its distinctness from E. moraniae we here propose the novel species Entomomonas asaccharolytica sp.
34516367	9	47	theme	quinone	792:798	arg1	system					800:805	The quinone system	788:805	The quinone system	788:805	The quinone system predominantly contained ubiquinone Q-8.
34516367	9	48	theme	ubiquinone	831:840	arg1	Q-8					842:844	ubiquinone Q-8	831:844	ubiquinone Q-8	831:844	The quinone system predominantly contained ubiquinone Q-8.
34516367	2	49	theme	polyphasic	148:157	arg1	characterization					169:184	a polyphasic taxonomic characterization	146:184	a polyphasic taxonomic characterization	146:184	Strain F2AT, isolated from the cricket Acheta domesticus, was subjected to a polyphasic taxonomic characterization.
34516367	8	50	theme	average	659:665	arg1	identity					678:685	average amino acid identity	659:685	average amino acid identity	659:685	Average nucleotide identity values based on blast and MUMmer and average amino acid identity values between strain F2AT and E. moraniae QZS01T were 74.29/74.43, 83.88 and 74.70 %, respectively.
34516367	6	51	theme	next	428:431	arg1	values					452:457	The next highest similarity values	424:457	The next highest similarity values	424:457	The next highest similarity values were found to representatives of related genera (<93 %).
34516367	8	52	theme	74.70 	765:770	arg1	%					771:771	74.29/74.43, 83.88 and 74.70 %	742:771	%	771:771	Average nucleotide identity values based on blast and MUMmer and average amino acid identity values between strain F2AT and E. moraniae QZS01T were 74.29/74.43, 83.88 and 74.70 %, respectively.
34516367	8	53	theme	strain	702:707	arg1	F2AT					709:712	strain F2AT	702:712	strain F2AT	702:712	Average nucleotide identity values based on blast and MUMmer and average amino acid identity values between strain F2AT and E. moraniae QZS01T were 74.29/74.43, 83.88 and 74.70 %, respectively.
34516367	13	54	theme	peptidoglycan	1231:1243	arg1	acid					1219:1222	The diagnostic diamino acid	1196:1222	The diagnostic diamino acid of the peptidoglycan	1196:1243	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
34516367	13	54	theme	peptidoglycan	1231:1243	arg1	acid					1269:1272	meso-diaminopimelic acid	1249:1272	meso-diaminopimelic acid	1249:1272	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
34516367	14	55	theme	Entomomonas	1417:1427	arg1	sp					1445:1446	Entomomonas asaccharolytica sp	1417:1446	the novel species Entomomonas asaccharolytica sp	1399:1446	Due to its association with the only species of the genus Entomomonas but its distinctness from E. moraniae we here propose the novel species Entomomonas asaccharolytica sp.
34516367	5	56	theme	Entomomonas	386:396	arg1	QZS01T					407:412	Entomomonas moraniae QZS01T	386:412	Entomomonas moraniae QZS01T (96.4 %)	386:421	The strain's 16S rRNA gene sequence showed highest similarity to Entomomonas moraniae QZS01T (96.4 %).
34516367	5	56	theme	Entomomonas	386:396	arg1	%					420:420	96.4 %	415:420	96.4 %	415:420	The strain's 16S rRNA gene sequence showed highest similarity to Entomomonas moraniae QZS01T (96.4 %).
34516367	8	57	theme	nucleotide	602:611	arg1	identity					613:620	Average nucleotide identity	594:620	Average nucleotide identity values based on blast and MUMmer and average amino acid identity values between strain F2AT and E. moraniae QZS01T	594:735	Average nucleotide identity values based on blast and MUMmer and average amino acid identity values between strain F2AT and E. moraniae QZS01T were 74.29/74.43, 83.88 and 74.70 %, respectively.
34516367	2	58	attach	isolated	84:91	arg1	domesticus					117:126	the cricket Acheta domesticus	98:126	the cricket Acheta domesticus	98:126	Strain F2AT, isolated from the cricket Acheta domesticus, was subjected to a polyphasic taxonomic characterization.
34516367	2	58	attach	isolated	84:91	arg2	F2AT					78:81	Strain F2AT	71:81	Strain F2AT	71:81	Strain F2AT, isolated from the cricket Acheta domesticus, was subjected to a polyphasic taxonomic characterization.
34516367	11	59	theme	polyamine	998:1006	arg1	pattern					1008:1014	The polyamine pattern	994:1014	The polyamine pattern	994:1014	The polyamine pattern consisted of the major compounds putrescine and spermidine.
34516367	5	60	theme	moraniae	398:405	arg1	QZS01T					407:412	Entomomonas moraniae QZS01T	386:412	Entomomonas moraniae QZS01T (96.4 %)	386:421	The strain's 16S rRNA gene sequence showed highest similarity to Entomomonas moraniae QZS01T (96.4 %).
34516367	5	60	theme	moraniae	398:405	arg1	%					420:420	96.4 %	415:420	96.4 %	415:420	The strain's 16S rRNA gene sequence showed highest similarity to Entomomonas moraniae QZS01T (96.4 %).
34516367	8	61	theme	amino	667:671	arg1	identity					678:685	average amino acid identity	659:685	average amino acid identity	659:685	Average nucleotide identity values based on blast and MUMmer and average amino acid identity values between strain F2AT and E. moraniae QZS01T were 74.29/74.43, 83.88 and 74.70 %, respectively.
34516367	14	62	dep	species	1409:1415	arg1	sp					1445:1446	Entomomonas asaccharolytica sp	1417:1446	the novel species Entomomonas asaccharolytica sp	1399:1446	Due to its association with the only species of the genus Entomomonas but its distinctness from E. moraniae we here propose the novel species Entomomonas asaccharolytica sp.
34516367	11	63	theme	major	1033:1037	arg1	spermidine					1064:1073	spermidine	1064:1073	spermidine	1064:1073	The polyamine pattern consisted of the major compounds putrescine and spermidine.
34516367	11	63	theme	major	1033:1037	arg1	putrescine					1049:1058	putrescine	1049:1058	putrescine	1049:1058	The polyamine pattern consisted of the major compounds putrescine and spermidine.
34516367	11	63	theme	major	1033:1037	arg1	compounds					1039:1047	the major compounds putrescine and spermidine	1029:1073	the major compounds putrescine and spermidine	1029:1073	The polyamine pattern consisted of the major compounds putrescine and spermidine.
34516367	14	64	theme	novel	1403:1407	arg1	species					1409:1415	the novel species	1399:1415	the novel species Entomomonas asaccharolytica sp	1399:1446	Due to its association with the only species of the genus Entomomonas but its distinctness from E. moraniae we here propose the novel species Entomomonas asaccharolytica sp.
34516367	13	65	theme	diamino	1211:1217	arg1	acid					1219:1222	The diagnostic diamino acid	1196:1222	The diagnostic diamino acid of the peptidoglycan	1196:1243	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
34516367	13	65	theme	diamino	1211:1217	arg1	acid					1269:1272	meso-diaminopimelic acid	1249:1272	meso-diaminopimelic acid	1249:1272	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
34516367	16	66	theme	9136T=LMG	1465:1473	arg1	32211T					1475:1480	=CCM 9136T=LMG 32211T	1460:1480	=CCM 9136T=LMG 32211T	1460:1480	F2AT (=CCM 9136T=LMG 32211T).
34516367	16	66	theme	9136T=LMG	1465:1473	arg1	F2AT					1454:1457	F2AT	1454:1457	F2AT (=CCM 9136T=LMG 32211T).	1454:1482	F2AT (=CCM 9136T=LMG 32211T).
34516367	8	67	theme	E.	718:719	arg1	QZS01T					730:735	E. moraniae QZS01T	718:735	E. moraniae QZS01T	718:735	Average nucleotide identity values based on blast and MUMmer and average amino acid identity values between strain F2AT and E. moraniae QZS01T were 74.29/74.43, 83.88 and 74.70 %, respectively.
34516367	14	68	with	association	1286:1296	arg1	species					1312:1318	the only species	1303:1318	the only species of the genus Entomomonas	1303:1343	Due to its association with the only species of the genus Entomomonas but its distinctness from E. moraniae we here propose the novel species Entomomonas asaccharolytica sp.
34516367	8	69	theme	moraniae	721:728	arg1	QZS01T					730:735	E. moraniae QZS01T	718:735	E. moraniae QZS01T	718:735	Average nucleotide identity values based on blast and MUMmer and average amino acid identity values between strain F2AT and E. moraniae QZS01T were 74.29/74.43, 83.88 and 74.70 %, respectively.
34208769	2	0	theme	hemocytes	582:590	arg1	drop					574:577	an initial dramatic drop	554:577	an initial dramatic drop of hemocytes	554:590	Injection of saline resulted in rapid recruitment of granular hemocytes from surrounding tissues, whereas laminarin injection on the other hand induced an initial dramatic drop of hemocytes.
34208769	6	1	theme	undescribed	1236:1246	arg1	inhibitor					1265:1273	a new and previously undescribed Kazal proteinase inhibitor	1215:1273	a new and previously undescribed Kazal proteinase inhibitor	1215:1273	All of them may be associated with immune responses, such as a new and previously undescribed Kazal proteinase inhibitor.
34208769	9	2	theme	other	1653:1657	arg1	crustaceans					1659:1669	other crustaceans	1653:1669	other crustaceans receiving injections with pathogens or pattern recognition proteins	1653:1737	This finding may demonstrate some problematic issues with gene and protein expression studies from other crustaceans receiving injections with pathogens or pattern recognition proteins.
34208769	1	3	theme	pattern	256:262	arg1	β-1,3-glucan					284:295	the fungal pattern recognition protein β-1,3-glucan	245:295	the fungal pattern recognition protein β-1,3-glucan (laminarin)	245:307	Early changes in hemocyte proteins in freshwater crayfish Pacifastacus leniusculus, in response to an injection with the fungal pattern recognition protein β-1,3-glucan (laminarin) were investigated, as well as changes after saline (vehicle) injection and in naïve animals.
34208769	1	3	theme	pattern	256:262	arg1	laminarin					298:306	laminarin	298:306	laminarin	298:306	Early changes in hemocyte proteins in freshwater crayfish Pacifastacus leniusculus, in response to an injection with the fungal pattern recognition protein β-1,3-glucan (laminarin) were investigated, as well as changes after saline (vehicle) injection and in naïve animals.
34208769	4	4	theme	present	826:832	arg1	proteins					817:824	proteins	817:824	proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1	817:995	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	4	5	theme	granular	837:844	arg1	protein					902:908	a vitelline membrane outer layer protein	869:908	a vitelline membrane outer layer protein 1 homolog	869:918	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	4	5	theme	granular	837:844	arg1	masquerade					945:954	masquerade	945:954	masquerade	945:954	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	4	5	theme	granular	837:844	arg1	homolog					987:993	serine protease homolog 1	971:995	serine protease homolog 1	971:995	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	4	5	theme	granular	837:844	arg1	crustin					957:963	crustin 1	957:965	crustin 1	957:965	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	4	5	theme	granular	837:844	arg1	vesicles					851:858	granular cell vesicles	837:858	granular cell vesicles	837:858	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	4	5	theme	granular	837:844	arg1	lectin					937:942	mannose-binding lectin	921:942	mannose-binding lectin	921:942	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	2	6	theme	other	535:539	arg1	hand					541:544	the other hand	531:544	the other hand	531:544	Injection of saline resulted in rapid recruitment of granular hemocytes from surrounding tissues, whereas laminarin injection on the other hand induced an initial dramatic drop of hemocytes.
34208769	1	7	theme	vehicle	361:367	arg1	injection					370:378	saline (vehicle) injection	353:378	saline (vehicle) injection	353:378	Early changes in hemocyte proteins in freshwater crayfish Pacifastacus leniusculus, in response to an injection with the fungal pattern recognition protein β-1,3-glucan (laminarin) were investigated, as well as changes after saline (vehicle) injection and in naïve animals.
34208769	9	8	theme	recognition	1718:1728	arg1	proteins					1730:1737	pattern recognition proteins	1710:1737	pattern recognition proteins	1710:1737	This finding may demonstrate some problematic issues with gene and protein expression studies from other crustaceans receiving injections with pathogens or pattern recognition proteins.
34208769	0	9	theme	Crayfish	17:24	arg1	Proteins					35:42	Crayfish Hemocyte Proteins	17:42	Crayfish Hemocyte Proteins	17:42	Early Changes in Crayfish Hemocyte Proteins after Injection with a β-1,3-glucan, Compared to Saline Injected and Naive Animals.
34208769	9	10	from	crustaceans	1659:1669	arg1	studies					1640:1646	gene and protein expression studies	1612:1646	studies	1640:1646	This finding may demonstrate some problematic issues with gene and protein expression studies from other crustaceans receiving injections with pathogens or pattern recognition proteins.
34208769	2	11	theme	initial	557:563	arg1	drop					574:577	an initial dramatic drop	554:577	an initial dramatic drop of hemocytes	554:590	Injection of saline resulted in rapid recruitment of granular hemocytes from surrounding tissues, whereas laminarin injection on the other hand induced an initial dramatic drop of hemocytes.
34208769	6	12	theme	immune	1189:1194	arg1	responses					1196:1204	immune responses	1189:1204	immune responses	1189:1204	All of them may be associated with immune responses, such as a new and previously undescribed Kazal proteinase inhibitor.
34208769	6	12	theme	immune	1189:1194	arg1	inhibitor					1265:1273	a new and previously undescribed Kazal proteinase inhibitor	1215:1273	a new and previously undescribed Kazal proteinase inhibitor	1215:1273	All of them may be associated with immune responses, such as a new and previously undescribed Kazal proteinase inhibitor.
34208769	8	13	theme	proteins	1446:1453	arg1	number					1413:1418	The number	1409:1418	The number of significantly affected proteins	1409:1453	The number of significantly affected proteins was very few after a laminarin injection when compared to uninjected and saline-injected crayfish.
34208769	8	13	theme	proteins	1446:1453	arg1	few					1464:1466	few	1464:1466	few	1464:1466	The number of significantly affected proteins was very few after a laminarin injection when compared to uninjected and saline-injected crayfish.
34208769	4	14	theme	membrane	881:888	arg1	protein					902:908	a vitelline membrane outer layer protein	869:908	a vitelline membrane outer layer protein 1 homolog	869:918	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	2	15	from	tissues	491:497	arg1	recruitment					440:450	rapid recruitment	434:450	rapid recruitment of granular hemocytes from surrounding tissues	434:497	Injection of saline resulted in rapid recruitment of granular hemocytes from surrounding tissues, whereas laminarin injection on the other hand induced an initial dramatic drop of hemocytes.
34208769	2	15	from	tissues	491:497	arg1	hemocytes					464:472	granular hemocytes	455:472	granular hemocytes from surrounding tissues	455:497	Injection of saline resulted in rapid recruitment of granular hemocytes from surrounding tissues, whereas laminarin injection on the other hand induced an initial dramatic drop of hemocytes.
34208769	6	16	theme	proteinase	1254:1263	arg1	inhibitor					1265:1273	a new and previously undescribed Kazal proteinase inhibitor	1215:1273	a new and previously undescribed Kazal proteinase inhibitor	1215:1273	All of them may be associated with immune responses, such as a new and previously undescribed Kazal proteinase inhibitor.
34208769	2	17	theme	surrounding	479:489	arg1	tissues					491:497	surrounding tissues	479:497	surrounding tissues	479:497	Injection of saline resulted in rapid recruitment of granular hemocytes from surrounding tissues, whereas laminarin injection on the other hand induced an initial dramatic drop of hemocytes.
34208769	9	18	theme	pattern	1710:1716	arg1	proteins					1730:1737	pattern recognition proteins	1710:1737	pattern recognition proteins	1710:1737	This finding may demonstrate some problematic issues with gene and protein expression studies from other crustaceans receiving injections with pathogens or pattern recognition proteins.
34208769	2	19	theme	hemocytes	464:472	arg1	recruitment					440:450	rapid recruitment	434:450	rapid recruitment of granular hemocytes from surrounding tissues	434:497	Injection of saline resulted in rapid recruitment of granular hemocytes from surrounding tissues, whereas laminarin injection on the other hand induced an initial dramatic drop of hemocytes.
34208769	4	20	from	vesicles	851:858	arg1	present					826:832	present	826:832	present	826:832	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	1	21	theme	hemocyte	145:152	arg1	proteins					154:161	hemocyte proteins	145:161	hemocyte proteins	145:161	Early changes in hemocyte proteins in freshwater crayfish Pacifastacus leniusculus, in response to an injection with the fungal pattern recognition protein β-1,3-glucan (laminarin) were investigated, as well as changes after saline (vehicle) injection and in naïve animals.
34208769	2	22	theme	laminarin	508:516	arg1	injection					518:526	laminarin injection	508:526	laminarin injection on the other hand	508:544	Injection of saline resulted in rapid recruitment of granular hemocytes from surrounding tissues, whereas laminarin injection on the other hand induced an initial dramatic drop of hemocytes.
34208769	8	23	theme	laminarin	1476:1484	arg1	injection					1486:1494	a laminarin injection	1474:1494	a laminarin injection	1474:1494	The number of significantly affected proteins was very few after a laminarin injection when compared to uninjected and saline-injected crayfish.
34208769	4	24	dep	protein	902:908	arg1	homolog					912:918	1 homolog	910:918	a vitelline membrane outer layer protein 1 homolog	869:918	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	4	25	theme	saline	762:767	arg1	injection					769:777	saline injection	762:777	saline injection	762:777	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	1	26	theme	saline	353:358	arg1	injection					370:378	saline (vehicle) injection	353:378	saline (vehicle) injection	353:378	Early changes in hemocyte proteins in freshwater crayfish Pacifastacus leniusculus, in response to an injection with the fungal pattern recognition protein β-1,3-glucan (laminarin) were investigated, as well as changes after saline (vehicle) injection and in naïve animals.
34208769	2	27	from	recruitment	440:450	arg1	tissues					491:497	surrounding tissues	479:497	surrounding tissues	479:497	Injection of saline resulted in rapid recruitment of granular hemocytes from surrounding tissues, whereas laminarin injection on the other hand induced an initial dramatic drop of hemocytes.
34208769	4	28	theme	granular	718:725	arg1	hemocytes					727:735	mature granular hemocytes	711:735	mature granular hemocytes	711:735	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	3	29	from	hours	600:604	arg1	composition					676:686	different composition	666:686	different composition	666:686	At six hours after injection, the hemocyte populations therefore were of different composition.
34208769	0	30	theme	Early	0:4	arg1	Changes					6:12	Early Changes	0:12	Early Changes in Crayfish Hemocyte Proteins	0:42	Early Changes in Crayfish Hemocyte Proteins after Injection with a β-1,3-glucan, Compared to Saline Injected and Naive Animals.
34208769	1	31	theme	recognition	264:274	arg1	β-1,3-glucan					284:295	the fungal pattern recognition protein β-1,3-glucan	245:295	the fungal pattern recognition protein β-1,3-glucan (laminarin)	245:307	Early changes in hemocyte proteins in freshwater crayfish Pacifastacus leniusculus, in response to an injection with the fungal pattern recognition protein β-1,3-glucan (laminarin) were investigated, as well as changes after saline (vehicle) injection and in naïve animals.
34208769	1	31	theme	recognition	264:274	arg1	laminarin					298:306	laminarin	298:306	laminarin	298:306	Early changes in hemocyte proteins in freshwater crayfish Pacifastacus leniusculus, in response to an injection with the fungal pattern recognition protein β-1,3-glucan (laminarin) were investigated, as well as changes after saline (vehicle) injection and in naïve animals.
34208769	4	32	theme	high	799:802	arg1	abundance					804:812	the high abundance	795:812	the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1	795:995	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	0	33	theme	Hemocyte	26:33	arg1	Proteins					35:42	Crayfish Hemocyte Proteins	17:42	Crayfish Hemocyte Proteins	17:42	Early Changes in Crayfish Hemocyte Proteins after Injection with a β-1,3-glucan, Compared to Saline Injected and Naive Animals.
34208769	5	34	theme	saline-injected	1105:1119	arg1	animals					1121:1127	saline-injected animals	1105:1127	saline-injected animals	1105:1127	After injection with the β-1,3-glucan, only three proteins were enhanced in expression, in comparison with saline-injected animals and uninjected controls.
34208769	9	35	theme	problematic	1588:1598	arg1	issues					1600:1605	some problematic issues	1583:1605	some problematic issues with gene and protein expression studies from other crustaceans receiving injections with pathogens or pattern recognition proteins	1583:1737	This finding may demonstrate some problematic issues with gene and protein expression studies from other crustaceans receiving injections with pathogens or pattern recognition proteins.
34208769	2	36	theme	rapid	434:438	arg1	recruitment					440:450	rapid recruitment	434:450	rapid recruitment of granular hemocytes from surrounding tissues	434:497	Injection of saline resulted in rapid recruitment of granular hemocytes from surrounding tissues, whereas laminarin injection on the other hand induced an initial dramatic drop of hemocytes.
34208769	2	37	from	injection	518:526	arg1	hand					541:544	the other hand	531:544	the other hand	531:544	Injection of saline resulted in rapid recruitment of granular hemocytes from surrounding tissues, whereas laminarin injection on the other hand induced an initial dramatic drop of hemocytes.
34208769	10	38	theme	uninjected	1746:1755	arg1	controls					1757:1764	no uninjected controls	1743:1764	no uninjected controls	1743:1764	If no uninjected controls are included and no information about hemocyte count (total or differential) is given, expressions data for proteins or mRNAs are very difficult to properly interpret.
34208769	4	39	theme	protease	978:985	arg1	homolog					987:993	serine protease homolog 1	971:995	serine protease homolog 1	971:995	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	9	40	theme	expression	1629:1638	arg1	studies					1640:1646	gene and protein expression studies	1612:1646	studies	1640:1646	This finding may demonstrate some problematic issues with gene and protein expression studies from other crustaceans receiving injections with pathogens or pattern recognition proteins.
34208769	6	41	theme	Kazal	1248:1252	arg1	inhibitor					1265:1273	a new and previously undescribed Kazal proteinase inhibitor	1215:1273	a new and previously undescribed Kazal proteinase inhibitor	1215:1273	All of them may be associated with immune responses, such as a new and previously undescribed Kazal proteinase inhibitor.
34208769	4	42	theme	cell	846:849	arg1	protein					902:908	a vitelline membrane outer layer protein	869:908	a vitelline membrane outer layer protein 1 homolog	869:918	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	4	42	theme	cell	846:849	arg1	masquerade					945:954	masquerade	945:954	masquerade	945:954	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	4	42	theme	cell	846:849	arg1	homolog					987:993	serine protease homolog 1	971:995	serine protease homolog 1	971:995	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	4	42	theme	cell	846:849	arg1	crustin					957:963	crustin 1	957:965	crustin 1	957:965	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	4	42	theme	cell	846:849	arg1	vesicles					851:858	granular cell vesicles	837:858	granular cell vesicles	837:858	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	4	42	theme	cell	846:849	arg1	lectin					937:942	mannose-binding lectin	921:942	mannose-binding lectin	921:942	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	9	43	with	issues	1600:1605	arg1	studies					1640:1646	gene and protein expression studies	1612:1646	studies	1640:1646	This finding may demonstrate some problematic issues with gene and protein expression studies from other crustaceans receiving injections with pathogens or pattern recognition proteins.
34208769	3	44	theme	different	666:674	arg1	composition					676:686	different composition	666:686	different composition	666:686	At six hours after injection, the hemocyte populations therefore were of different composition.
34208769	1	45	theme	fungal	249:254	arg1	β-1,3-glucan					284:295	the fungal pattern recognition protein β-1,3-glucan	245:295	the fungal pattern recognition protein β-1,3-glucan (laminarin)	245:307	Early changes in hemocyte proteins in freshwater crayfish Pacifastacus leniusculus, in response to an injection with the fungal pattern recognition protein β-1,3-glucan (laminarin) were investigated, as well as changes after saline (vehicle) injection and in naïve animals.
34208769	1	45	theme	fungal	249:254	arg1	laminarin					298:306	laminarin	298:306	laminarin	298:306	Early changes in hemocyte proteins in freshwater crayfish Pacifastacus leniusculus, in response to an injection with the fungal pattern recognition protein β-1,3-glucan (laminarin) were investigated, as well as changes after saline (vehicle) injection and in naïve animals.
34208769	2	46	theme	dramatic	565:572	arg1	drop					574:577	an initial dramatic drop	554:577	an initial dramatic drop of hemocytes	554:590	Injection of saline resulted in rapid recruitment of granular hemocytes from surrounding tissues, whereas laminarin injection on the other hand induced an initial dramatic drop of hemocytes.
34208769	6	47	theme	new	1217:1219	arg1	inhibitor					1265:1273	a new and previously undescribed Kazal proteinase inhibitor	1215:1273	a new and previously undescribed Kazal proteinase inhibitor	1215:1273	All of them may be associated with immune responses, such as a new and previously undescribed Kazal proteinase inhibitor.
34208769	4	48	theme	proteins	817:824	arg1	abundance					804:812	the high abundance	795:812	the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1	795:995	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	10	49	theme	hemocyte	1804:1811	arg1	count					1813:1817	hemocyte count	1804:1817	hemocyte count (total or differential)	1804:1841	If no uninjected controls are included and no information about hemocyte count (total or differential) is given, expressions data for proteins or mRNAs are very difficult to properly interpret.
34208769	1	50	theme	freshwater	166:175	arg1	crayfish					177:184	freshwater crayfish	166:184	freshwater crayfish	166:184	Early changes in hemocyte proteins in freshwater crayfish Pacifastacus leniusculus, in response to an injection with the fungal pattern recognition protein β-1,3-glucan (laminarin) were investigated, as well as changes after saline (vehicle) injection and in naïve animals.
34208769	0	51	theme	Naive	113:117	arg1	Animals					119:125	Saline Injected and Naive Animals	93:125	Saline Injected and Naive Animals	93:125	Early Changes in Crayfish Hemocyte Proteins after Injection with a β-1,3-glucan, Compared to Saline Injected and Naive Animals.
34208769	4	52	theme	outer	890:894	arg1	protein					902:908	a vitelline membrane outer layer protein	869:908	a vitelline membrane outer layer protein 1 homolog	869:918	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	10	53	dep	count	1813:1817	arg1	total					1820:1824	total	1820:1824	total	1820:1824	If no uninjected controls are included and no information about hemocyte count (total or differential) is given, expressions data for proteins or mRNAs are very difficult to properly interpret.
34208769	10	53	dep	count	1813:1817	arg1	differential					1829:1840	differential	1829:1840	differential	1829:1840	If no uninjected controls are included and no information about hemocyte count (total or differential) is given, expressions data for proteins or mRNAs are very difficult to properly interpret.
34208769	8	54	theme	affected	1437:1444	arg1	proteins					1446:1453	significantly affected proteins	1423:1453	significantly affected proteins	1423:1453	The number of significantly affected proteins was very few after a laminarin injection when compared to uninjected and saline-injected crayfish.
34208769	4	55	theme	layer	896:900	arg1	protein					902:908	a vitelline membrane outer layer protein	869:908	a vitelline membrane outer layer protein 1 homolog	869:918	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	1	56	theme	naïve	387:391	arg1	animals					393:399	naïve animals	387:399	naïve animals	387:399	Early changes in hemocyte proteins in freshwater crayfish Pacifastacus leniusculus, in response to an injection with the fungal pattern recognition protein β-1,3-glucan (laminarin) were investigated, as well as changes after saline (vehicle) injection and in naïve animals.
34208769	4	57	theme	vitelline	871:879	arg1	protein					902:908	a vitelline membrane outer layer protein	869:908	a vitelline membrane outer layer protein 1 homolog	869:918	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	1	58	from	crayfish	177:184	arg1	response					215:222	response	215:222	response to an injection with the fungal pattern recognition protein β-1,3-glucan (laminarin)	215:307	Early changes in hemocyte proteins in freshwater crayfish Pacifastacus leniusculus, in response to an injection with the fungal pattern recognition protein β-1,3-glucan (laminarin) were investigated, as well as changes after saline (vehicle) injection and in naïve animals.
34208769	1	59	theme	Early	128:132	arg1	changes					134:140	Early changes	128:140	Early changes in hemocyte proteins in freshwater crayfish Pacifastacus leniusculus, in response to an injection with the fungal pattern recognition protein β-1,3-glucan (laminarin)	128:307	Early changes in hemocyte proteins in freshwater crayfish Pacifastacus leniusculus, in response to an injection with the fungal pattern recognition protein β-1,3-glucan (laminarin) were investigated, as well as changes after saline (vehicle) injection and in naïve animals.
34208769	7	60	theme	clotting	1317:1324	arg1	protein					1326:1332	the clotting protein	1313:1332	the clotting protein	1313:1332	One interesting observation was that the clotting protein was increased dramatically in most of the animals injected with laminarin.
34208769	5	61	with	comparison	1089:1098	arg1	animals					1121:1127	saline-injected animals	1105:1127	saline-injected animals	1105:1127	After injection with the β-1,3-glucan, only three proteins were enhanced in expression, in comparison with saline-injected animals and uninjected controls.
34208769	5	61	with	comparison	1089:1098	arg1	controls					1144:1151	uninjected controls	1133:1151	uninjected controls	1133:1151	After injection with the β-1,3-glucan, only three proteins were enhanced in expression, in comparison with saline-injected animals and uninjected controls.
34208769	4	62	attach	present	826:832	arg1	protein					902:908	a vitelline membrane outer layer protein	869:908	a vitelline membrane outer layer protein 1 homolog	869:918	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	4	62	attach	present	826:832	arg1	masquerade					945:954	masquerade	945:954	masquerade	945:954	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	4	62	attach	present	826:832	arg1	homolog					987:993	serine protease homolog 1	971:995	serine protease homolog 1	971:995	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	4	62	attach	present	826:832	arg1	crustin					957:963	crustin 1	957:965	crustin 1	957:965	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	4	62	attach	present	826:832	arg1	vesicles					851:858	granular cell vesicles	837:858	granular cell vesicles	837:858	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	4	62	attach	present	826:832	arg2	proteins					817:824	proteins	817:824	proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1	817:995	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	4	62	attach	present	826:832	arg1	lectin					937:942	mannose-binding lectin	921:942	mannose-binding lectin	921:942	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	2	63	theme	granular	455:462	arg1	hemocytes					464:472	granular hemocytes	455:472	granular hemocytes from surrounding tissues	455:497	Injection of saline resulted in rapid recruitment of granular hemocytes from surrounding tissues, whereas laminarin injection on the other hand induced an initial dramatic drop of hemocytes.
34208769	0	64	from	Changes	6:12	arg1	Proteins					35:42	Crayfish Hemocyte Proteins	17:42	Crayfish Hemocyte Proteins	17:42	Early Changes in Crayfish Hemocyte Proteins after Injection with a β-1,3-glucan, Compared to Saline Injected and Naive Animals.
34208769	0	65	dep	Injected	100:107	arg1	Saline					93:98	Saline	93:98	Saline	93:98	Early Changes in Crayfish Hemocyte Proteins after Injection with a β-1,3-glucan, Compared to Saline Injected and Naive Animals.
34208769	0	66	theme	Injected	100:107	arg1	Animals					119:125	Saline Injected and Naive Animals	93:125	Saline Injected and Naive Animals	93:125	Early Changes in Crayfish Hemocyte Proteins after Injection with a β-1,3-glucan, Compared to Saline Injected and Naive Animals.
34208769	1	67	from	changes	134:140	arg1	proteins					154:161	hemocyte proteins	145:161	hemocyte proteins	145:161	Early changes in hemocyte proteins in freshwater crayfish Pacifastacus leniusculus, in response to an injection with the fungal pattern recognition protein β-1,3-glucan (laminarin) were investigated, as well as changes after saline (vehicle) injection and in naïve animals.
34208769	1	67	from	changes	134:140	arg1	crayfish					177:184	freshwater crayfish	166:184	freshwater crayfish	166:184	Early changes in hemocyte proteins in freshwater crayfish Pacifastacus leniusculus, in response to an injection with the fungal pattern recognition protein β-1,3-glucan (laminarin) were investigated, as well as changes after saline (vehicle) injection and in naïve animals.
34208769	4	68	theme	mannose-binding	921:935	arg1	lectin					937:942	mannose-binding lectin	921:942	mannose-binding lectin	921:942	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	8	69	theme	uninjected	1513:1522	arg1	crayfish					1544:1551	uninjected and saline-injected crayfish	1513:1551	uninjected and saline-injected crayfish	1513:1551	The number of significantly affected proteins was very few after a laminarin injection when compared to uninjected and saline-injected crayfish.
34208769	5	70	with	injection	1004:1012	arg1	β-1,3-glucan					1023:1034	the β-1,3-glucan	1019:1034	the β-1,3-glucan	1019:1034	After injection with the β-1,3-glucan, only three proteins were enhanced in expression, in comparison with saline-injected animals and uninjected controls.
34208769	7	71	theme	interesting	1280:1290	arg1	observation					1292:1302	One interesting observation	1276:1302	One interesting observation	1276:1302	One interesting observation was that the clotting protein was increased dramatically in most of the animals injected with laminarin.
34208769	2	72	theme	saline	415:420	arg1	Injection					402:410	Injection	402:410	Injection of saline	402:420	Injection of saline resulted in rapid recruitment of granular hemocytes from surrounding tissues, whereas laminarin injection on the other hand induced an initial dramatic drop of hemocytes.
34208769	8	73	theme	saline-injected	1528:1542	arg1	crayfish					1544:1551	uninjected and saline-injected crayfish	1513:1551	uninjected and saline-injected crayfish	1513:1551	The number of significantly affected proteins was very few after a laminarin injection when compared to uninjected and saline-injected crayfish.
34208769	4	74	theme	mature	711:716	arg1	hemocytes					727:735	mature granular hemocytes	711:735	mature granular hemocytes	711:735	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	1	75	from	changes	339:345	arg1	animals					393:399	naïve animals	387:399	naïve animals	387:399	Early changes in hemocyte proteins in freshwater crayfish Pacifastacus leniusculus, in response to an injection with the fungal pattern recognition protein β-1,3-glucan (laminarin) were investigated, as well as changes after saline (vehicle) injection and in naïve animals.
34208769	1	76	theme	protein	276:282	arg1	β-1,3-glucan					284:295	the fungal pattern recognition protein β-1,3-glucan	245:295	the fungal pattern recognition protein β-1,3-glucan (laminarin)	245:307	Early changes in hemocyte proteins in freshwater crayfish Pacifastacus leniusculus, in response to an injection with the fungal pattern recognition protein β-1,3-glucan (laminarin) were investigated, as well as changes after saline (vehicle) injection and in naïve animals.
34208769	1	76	theme	protein	276:282	arg1	laminarin					298:306	laminarin	298:306	laminarin	298:306	Early changes in hemocyte proteins in freshwater crayfish Pacifastacus leniusculus, in response to an injection with the fungal pattern recognition protein β-1,3-glucan (laminarin) were investigated, as well as changes after saline (vehicle) injection and in naïve animals.
34208769	10	77	theme	expressions	1853:1863	arg1	data					1865:1868	expressions data	1853:1868	expressions data for proteins or mRNAs	1853:1890	If no uninjected controls are included and no information about hemocyte count (total or differential) is given, expressions data for proteins or mRNAs are very difficult to properly interpret.
34208769	4	78	from	present	826:832	arg1	protein					902:908	a vitelline membrane outer layer protein	869:908	a vitelline membrane outer layer protein 1 homolog	869:918	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	4	78	from	present	826:832	arg1	masquerade					945:954	masquerade	945:954	masquerade	945:954	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	4	78	from	present	826:832	arg1	homolog					987:993	serine protease homolog 1	971:995	serine protease homolog 1	971:995	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	4	78	from	present	826:832	arg1	crustin					957:963	crustin 1	957:965	crustin 1	957:965	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	4	78	from	present	826:832	arg1	vesicles					851:858	granular cell vesicles	837:858	granular cell vesicles	837:858	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	4	78	from	present	826:832	arg1	lectin					937:942	mannose-binding lectin	921:942	mannose-binding lectin	921:942	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	4	79	theme	serine	971:976	arg1	homolog					987:993	serine protease homolog 1	971:995	serine protease homolog 1	971:995	The results show that mature granular hemocytes increase in number after saline injection as indicated by the high abundance of proteins present in granular cell vesicles, such as a vitelline membrane outer layer protein 1 homolog, mannose-binding lectin, masquerade, crustin 1 and serine protease homolog 1.
34208769	5	80	theme	uninjected	1133:1142	arg1	controls					1144:1151	uninjected controls	1133:1151	uninjected controls	1133:1151	After injection with the β-1,3-glucan, only three proteins were enhanced in expression, in comparison with saline-injected animals and uninjected controls.
34208769	9	81	theme	gene	1612:1615	arg1	studies					1640:1646	gene and protein expression studies	1612:1646	studies	1640:1646	This finding may demonstrate some problematic issues with gene and protein expression studies from other crustaceans receiving injections with pathogens or pattern recognition proteins.
34208769	3	82	theme	hemocyte	627:634	arg1	populations					636:646	the hemocyte populations	623:646	the hemocyte populations	623:646	At six hours after injection, the hemocyte populations therefore were of different composition.
34208769	0	83	with	Injection	50:58	arg1	β-1,3-glucan					67:78	a β-1,3-glucan	65:78	a β-1,3-glucan	65:78	Early Changes in Crayfish Hemocyte Proteins after Injection with a β-1,3-glucan, Compared to Saline Injected and Naive Animals.
34208769	9	84	with	injections	1681:1690	arg1	proteins					1730:1737	pattern recognition proteins	1710:1737	pattern recognition proteins	1710:1737	This finding may demonstrate some problematic issues with gene and protein expression studies from other crustaceans receiving injections with pathogens or pattern recognition proteins.
34208769	9	84	with	injections	1681:1690	arg1	pathogens					1697:1705	pathogens	1697:1705	pathogens	1697:1705	This finding may demonstrate some problematic issues with gene and protein expression studies from other crustaceans receiving injections with pathogens or pattern recognition proteins.
34208769	9	85	theme	protein	1621:1627	arg1	studies					1640:1646	gene and protein expression studies	1612:1646	studies	1640:1646	This finding may demonstrate some problematic issues with gene and protein expression studies from other crustaceans receiving injections with pathogens or pattern recognition proteins.
34208769	1	86	with	injection	230:238	arg1	β-1,3-glucan					284:295	the fungal pattern recognition protein β-1,3-glucan	245:295	the fungal pattern recognition protein β-1,3-glucan (laminarin)	245:307	Early changes in hemocyte proteins in freshwater crayfish Pacifastacus leniusculus, in response to an injection with the fungal pattern recognition protein β-1,3-glucan (laminarin) were investigated, as well as changes after saline (vehicle) injection and in naïve animals.
34208769	1	86	with	injection	230:238	arg1	laminarin					298:306	laminarin	298:306	laminarin	298:306	Early changes in hemocyte proteins in freshwater crayfish Pacifastacus leniusculus, in response to an injection with the fungal pattern recognition protein β-1,3-glucan (laminarin) were investigated, as well as changes after saline (vehicle) injection and in naïve animals.
34044918	4	0	theme	metabolite	679:688	arg1	profiles					690:697	metabolite profiles	679:697	metabolite profiles in ovariectomized rat faeces	679:726	16S rRNA sequencing and metabolomics analysis indicated CSCa intervention altered the composition of gut microbiota along with metabolite profiles in ovariectomized rat faeces.
34044918	2	1	theme	osteoporosis	331:342	arg1	rats					344:347	osteoporosis rats	331:347	osteoporosis rats	331:347	Here, the effects of CSCa on osteoporosis rats were investigated.
34044918	6	2	theme	metabolite	1027:1036	arg1	profiles					1038:1045	the metabolite profiles	1023:1045	the metabolite profiles	1023:1045	Taken together, dietary CSCa intervention has the potential to alleviate the osteoporosis and related symptoms probably involving gut microbiota or the metabolite profiles as demonstrated in rats.
34044918	5	3	theme	gut	766:768	arg1	taxa					781:784	some gut microbiota taxa	761:784	some gut microbiota taxa	761:784	The correlation analysis showed some gut microbiota taxa were significantly correlated with osteoporosis phenotypes and the enriched metabolites.
34044918	2	4	from	effects	312:318	arg1	rats					344:347	osteoporosis rats	331:347	osteoporosis rats	331:347	Here, the effects of CSCa on osteoporosis rats were investigated.
34044918	7	5	theme	food	1158:1161	arg1	supplement					1163:1172	the food supplement	1154:1172	the food supplement	1154:1172	This study provides some scientific evidence for the potential effects of CSCa as the food supplement on the osteoporosis.
34044918	1	6	theme	anti-osteoporosis	243:259	arg1	bioactivity					261:271	its anti-osteoporosis bioactivity	239:271	its anti-osteoporosis bioactivity	239:271	Although chondroitin sulfate calcium complex (CSCa) was claimed to have the bioactivity for bone care in vitro, its anti-osteoporosis bioactivity was little reported in vivo.
34044918	0	7	theme	osteoporosis	90:101	arg1	alleviation					75:85	alleviation	75:85	alleviation of osteoporosis in ovariectomized rats	75:124	Modulation of gut microbiota by chondroitin sulfate calcium complex during alleviation of osteoporosis in ovariectomized rats.
34044918	7	8	theme	CSCa	1146:1149	arg1	effects					1135:1141	the potential effects	1121:1141	the potential effects of CSCa as the food supplement on the osteoporosis	1121:1192	This study provides some scientific evidence for the potential effects of CSCa as the food supplement on the osteoporosis.
34044918	3	9	theme	bone	514:517	arg1	markers					528:534	bone turnover markers	514:534	the bone turnover markers level in serum	510:549	Results showed that, compared with the osteoporosis rats, CSCa could improve the bone mineral density and microstructure of femur, and change the bone turnover markers level in serum.
34044918	3	10	theme	mineral	454:460	arg1	density					462:468	bone mineral density	449:468	bone mineral density	449:468	Results showed that, compared with the osteoporosis rats, CSCa could improve the bone mineral density and microstructure of femur, and change the bone turnover markers level in serum.
34044918	7	11	theme	potential	1125:1133	arg1	effects					1135:1141	the potential effects	1121:1141	the potential effects of CSCa as the food supplement on the osteoporosis	1121:1192	This study provides some scientific evidence for the potential effects of CSCa as the food supplement on the osteoporosis.
34044918	3	12	dep	density	462:468	arg1	the					445:447	the	445:447	the	445:447	Results showed that, compared with the osteoporosis rats, CSCa could improve the bone mineral density and microstructure of femur, and change the bone turnover markers level in serum.
34044918	0	13	theme	ovariectomized	106:119	arg1	rats					121:124	ovariectomized rats	106:124	ovariectomized rats	106:124	Modulation of gut microbiota by chondroitin sulfate calcium complex during alleviation of osteoporosis in ovariectomized rats.
34044918	6	14	theme	CSCa	899:902	arg1	intervention					904:915	dietary CSCa intervention	891:915	dietary CSCa intervention	891:915	Taken together, dietary CSCa intervention has the potential to alleviate the osteoporosis and related symptoms probably involving gut microbiota or the metabolite profiles as demonstrated in rats.
34044918	5	15	theme	microbiota	770:779	arg1	taxa					781:784	some gut microbiota taxa	761:784	some gut microbiota taxa	761:784	The correlation analysis showed some gut microbiota taxa were significantly correlated with osteoporosis phenotypes and the enriched metabolites.
34044918	6	16	theme	dietary	891:897	arg1	intervention					904:915	dietary CSCa intervention	891:915	dietary CSCa intervention	891:915	Taken together, dietary CSCa intervention has the potential to alleviate the osteoporosis and related symptoms probably involving gut microbiota or the metabolite profiles as demonstrated in rats.
34044918	4	17	theme	gut	653:655	arg1	microbiota					657:666	gut microbiota	653:666	gut microbiota	653:666	16S rRNA sequencing and metabolomics analysis indicated CSCa intervention altered the composition of gut microbiota along with metabolite profiles in ovariectomized rat faeces.
34044918	6	18	theme	gut	1005:1007	arg1	microbiota					1009:1018	gut microbiota	1005:1018	gut microbiota	1005:1018	Taken together, dietary CSCa intervention has the potential to alleviate the osteoporosis and related symptoms probably involving gut microbiota or the metabolite profiles as demonstrated in rats.
34044918	4	19	theme	ovariectomized	702:715	arg1	faeces					721:726	ovariectomized rat faeces	702:726	ovariectomized rat faeces	702:726	16S rRNA sequencing and metabolomics analysis indicated CSCa intervention altered the composition of gut microbiota along with metabolite profiles in ovariectomized rat faeces.
34044918	3	20	theme	osteoporosis	407:418	arg1	rats					420:423	the osteoporosis rats	403:423	the osteoporosis rats	403:423	Results showed that, compared with the osteoporosis rats, CSCa could improve the bone mineral density and microstructure of femur, and change the bone turnover markers level in serum.
34044918	0	21	theme	microbiota	18:27	arg1	Modulation					0:9	Modulation	0:9	Modulation of gut microbiota by chondroitin sulfate calcium complex during alleviation of osteoporosis in ovariectomized rats.	0:125	Modulation of gut microbiota by chondroitin sulfate calcium complex during alleviation of osteoporosis in ovariectomized rats.
34044918	7	22	theme	scientific	1097:1106	arg1	evidence					1108:1115	some scientific evidence	1092:1115	some scientific evidence for the potential effects of CSCa as the food supplement on the osteoporosis	1092:1192	This study provides some scientific evidence for the potential effects of CSCa as the food supplement on the osteoporosis.
34044918	3	23	theme	turnover	519:526	arg1	markers					528:534	bone turnover markers	514:534	the bone turnover markers level in serum	510:549	Results showed that, compared with the osteoporosis rats, CSCa could improve the bone mineral density and microstructure of femur, and change the bone turnover markers level in serum.
34044918	4	24	theme	CSCa	608:611	arg1	intervention					613:624	CSCa intervention	608:624	CSCa intervention	608:624	16S rRNA sequencing and metabolomics analysis indicated CSCa intervention altered the composition of gut microbiota along with metabolite profiles in ovariectomized rat faeces.
34044918	0	25	theme	gut	14:16	arg1	microbiota					18:27	gut microbiota	14:27	gut microbiota	14:27	Modulation of gut microbiota by chondroitin sulfate calcium complex during alleviation of osteoporosis in ovariectomized rats.
34044918	6	26	dep	osteoporosis	952:963	arg1	the					948:950	the	948:950	the	948:950	Taken together, dietary CSCa intervention has the potential to alleviate the osteoporosis and related symptoms probably involving gut microbiota or the metabolite profiles as demonstrated in rats.
34044918	6	26	dep	osteoporosis	952:963	arg1	demonstrated					1050:1061	demonstrated	1050:1061	demonstrated in rats	1050:1069	Taken together, dietary CSCa intervention has the potential to alleviate the osteoporosis and related symptoms probably involving gut microbiota or the metabolite profiles as demonstrated in rats.
34044918	3	27	theme	markers	528:534	arg1	level					536:540	the bone turnover markers level	510:540	the bone turnover markers level in serum	510:549	Results showed that, compared with the osteoporosis rats, CSCa could improve the bone mineral density and microstructure of femur, and change the bone turnover markers level in serum.
34044918	3	28	theme	femur	492:496	arg1	density					462:468	bone mineral density	449:468	bone mineral density	449:468	Results showed that, compared with the osteoporosis rats, CSCa could improve the bone mineral density and microstructure of femur, and change the bone turnover markers level in serum.
34044918	3	28	theme	femur	492:496	arg1	microstructure					474:487	microstructure	474:487	microstructure	474:487	Results showed that, compared with the osteoporosis rats, CSCa could improve the bone mineral density and microstructure of femur, and change the bone turnover markers level in serum.
34044918	0	29	theme	chondroitin	32:42	arg1	complex					60:66	chondroitin sulfate calcium complex	32:66	chondroitin sulfate calcium complex during alleviation of osteoporosis in ovariectomized rats	32:124	Modulation of gut microbiota by chondroitin sulfate calcium complex during alleviation of osteoporosis in ovariectomized rats.
34044918	7	30	from	effects	1135:1141	arg1	osteoporosis					1181:1192	the osteoporosis	1177:1192	the osteoporosis	1177:1192	This study provides some scientific evidence for the potential effects of CSCa as the food supplement on the osteoporosis.
34044918	0	31	from	alleviation	75:85	arg1	rats					121:124	ovariectomized rats	106:124	ovariectomized rats	106:124	Modulation of gut microbiota by chondroitin sulfate calcium complex during alleviation of osteoporosis in ovariectomized rats.
34044918	5	32	theme	enriched	853:860	arg1	metabolites					862:872	the enriched metabolites	849:872	the enriched metabolites	849:872	The correlation analysis showed some gut microbiota taxa were significantly correlated with osteoporosis phenotypes and the enriched metabolites.
34044918	6	33	theme	related	969:975	arg1	symptoms					977:984	related symptoms	969:984	related symptoms	969:984	Taken together, dietary CSCa intervention has the potential to alleviate the osteoporosis and related symptoms probably involving gut microbiota or the metabolite profiles as demonstrated in rats.
34044918	5	34	dep	showed	754:759	arg1	correlated					805:814	correlated	805:814	showed some gut microbiota taxa were significantly correlated with osteoporosis phenotypes and the enriched metabolites	754:872	The correlation analysis showed some gut microbiota taxa were significantly correlated with osteoporosis phenotypes and the enriched metabolites.
34044918	5	35	theme	osteoporosis	821:832	arg1	phenotypes					834:843	osteoporosis phenotypes	821:843	osteoporosis phenotypes	821:843	The correlation analysis showed some gut microbiota taxa were significantly correlated with osteoporosis phenotypes and the enriched metabolites.
34044918	5	36	theme	correlation	733:743	arg1	analysis					745:752	The correlation analysis	729:752	The correlation analysis	729:752	The correlation analysis showed some gut microbiota taxa were significantly correlated with osteoporosis phenotypes and the enriched metabolites.
34044918	0	37	theme	calcium	52:58	arg1	complex					60:66	chondroitin sulfate calcium complex	32:66	chondroitin sulfate calcium complex during alleviation of osteoporosis in ovariectomized rats	32:124	Modulation of gut microbiota by chondroitin sulfate calcium complex during alleviation of osteoporosis in ovariectomized rats.
34044918	2	38	theme	CSCa	323:326	arg1	effects					312:318	the effects	308:318	the effects of CSCa on osteoporosis rats	308:347	Here, the effects of CSCa on osteoporosis rats were investigated.
34044918	0	39	theme	sulfate	44:50	arg1	complex					60:66	chondroitin sulfate calcium complex	32:66	chondroitin sulfate calcium complex during alleviation of osteoporosis in ovariectomized rats	32:124	Modulation of gut microbiota by chondroitin sulfate calcium complex during alleviation of osteoporosis in ovariectomized rats.
34044918	4	40	dep	indicated	598:606	arg1	altered					626:632	altered	626:632	indicated CSCa intervention altered the composition of gut microbiota along with metabolite profiles in ovariectomized rat faeces	598:726	16S rRNA sequencing and metabolomics analysis indicated CSCa intervention altered the composition of gut microbiota along with metabolite profiles in ovariectomized rat faeces.
34044918	4	41	theme	rat	717:719	arg1	faeces					721:726	ovariectomized rat faeces	702:726	ovariectomized rat faeces	702:726	16S rRNA sequencing and metabolomics analysis indicated CSCa intervention altered the composition of gut microbiota along with metabolite profiles in ovariectomized rat faeces.
34044918	1	42	theme	chondroitin	136:146	arg1	complex					164:170	chondroitin sulfate calcium complex	136:170	chondroitin sulfate calcium complex (CSCa)	136:177	Although chondroitin sulfate calcium complex (CSCa) was claimed to have the bioactivity for bone care in vitro, its anti-osteoporosis bioactivity was little reported in vivo.
34044918	1	42	theme	chondroitin	136:146	arg1	CSCa					173:176	CSCa	173:176	CSCa	173:176	Although chondroitin sulfate calcium complex (CSCa) was claimed to have the bioactivity for bone care in vitro, its anti-osteoporosis bioactivity was little reported in vivo.
34044918	1	43	theme	bone	219:222	arg1	care					224:227	bone care	219:227	bone care	219:227	Although chondroitin sulfate calcium complex (CSCa) was claimed to have the bioactivity for bone care in vitro, its anti-osteoporosis bioactivity was little reported in vivo.
34044918	1	44	theme	sulfate	148:154	arg1	complex					164:170	chondroitin sulfate calcium complex	136:170	chondroitin sulfate calcium complex (CSCa)	136:177	Although chondroitin sulfate calcium complex (CSCa) was claimed to have the bioactivity for bone care in vitro, its anti-osteoporosis bioactivity was little reported in vivo.
34044918	1	44	theme	sulfate	148:154	arg1	CSCa					173:176	CSCa	173:176	CSCa	173:176	Although chondroitin sulfate calcium complex (CSCa) was claimed to have the bioactivity for bone care in vitro, its anti-osteoporosis bioactivity was little reported in vivo.
34044918	3	45	from	level	536:540	arg1	serum					545:549	serum	545:549	serum	545:549	Results showed that, compared with the osteoporosis rats, CSCa could improve the bone mineral density and microstructure of femur, and change the bone turnover markers level in serum.
34044918	3	46	theme	bone	449:452	arg1	density					462:468	bone mineral density	449:468	bone mineral density	449:468	Results showed that, compared with the osteoporosis rats, CSCa could improve the bone mineral density and microstructure of femur, and change the bone turnover markers level in serum.
34044918	1	47	contain	have	194:197	arg1	complex					164:170	chondroitin sulfate calcium complex	136:170	chondroitin sulfate calcium complex (CSCa)	136:177	Although chondroitin sulfate calcium complex (CSCa) was claimed to have the bioactivity for bone care in vitro, its anti-osteoporosis bioactivity was little reported in vivo.
34044918	1	47	contain	have	194:197	arg2	bioactivity					203:213	the bioactivity	199:213	the bioactivity for bone care	199:227	Although chondroitin sulfate calcium complex (CSCa) was claimed to have the bioactivity for bone care in vitro, its anti-osteoporosis bioactivity was little reported in vivo.
34044918	1	47	contain	have	194:197	arg1	CSCa					173:176	CSCa	173:176	CSCa	173:176	Although chondroitin sulfate calcium complex (CSCa) was claimed to have the bioactivity for bone care in vitro, its anti-osteoporosis bioactivity was little reported in vivo.
34044918	6	48	contain	has	917:919	arg1	intervention					904:915	dietary CSCa intervention	891:915	dietary CSCa intervention	891:915	Taken together, dietary CSCa intervention has the potential to alleviate the osteoporosis and related symptoms probably involving gut microbiota or the metabolite profiles as demonstrated in rats.
34044918	6	48	contain	has	917:919	arg2	potential					925:933	the potential to alleviate the osteoporosis and related symptoms probably involving gut microbiota or the metabolite profiles as demonstrated in rats	921:1069	the potential to alleviate the osteoporosis and related symptoms probably involving gut microbiota or the metabolite profiles as demonstrated in rats	921:1069	Taken together, dietary CSCa intervention has the potential to alleviate the osteoporosis and related symptoms probably involving gut microbiota or the metabolite profiles as demonstrated in rats.
34044918	1	49	theme	calcium	156:162	arg1	complex					164:170	chondroitin sulfate calcium complex	136:170	chondroitin sulfate calcium complex (CSCa)	136:177	Although chondroitin sulfate calcium complex (CSCa) was claimed to have the bioactivity for bone care in vitro, its anti-osteoporosis bioactivity was little reported in vivo.
34044918	1	49	theme	calcium	156:162	arg1	CSCa					173:176	CSCa	173:176	CSCa	173:176	Although chondroitin sulfate calcium complex (CSCa) was claimed to have the bioactivity for bone care in vitro, its anti-osteoporosis bioactivity was little reported in vivo.
34044918	4	50	from	profiles	690:697	arg1	faeces					721:726	ovariectomized rat faeces	702:726	ovariectomized rat faeces	702:726	16S rRNA sequencing and metabolomics analysis indicated CSCa intervention altered the composition of gut microbiota along with metabolite profiles in ovariectomized rat faeces.
34044918	4	51	theme	16S	552:554	arg1	sequencing					561:570	16S rRNA sequencing	552:570	16S rRNA sequencing	552:570	16S rRNA sequencing and metabolomics analysis indicated CSCa intervention altered the composition of gut microbiota along with metabolite profiles in ovariectomized rat faeces.
34044918	4	52	theme	rRNA	556:559	arg1	sequencing					561:570	16S rRNA sequencing	552:570	16S rRNA sequencing	552:570	16S rRNA sequencing and metabolomics analysis indicated CSCa intervention altered the composition of gut microbiota along with metabolite profiles in ovariectomized rat faeces.
34044918	4	53	theme	microbiota	657:666	arg1	composition					638:648	the composition	634:648	the composition of gut microbiota	634:666	16S rRNA sequencing and metabolomics analysis indicated CSCa intervention altered the composition of gut microbiota along with metabolite profiles in ovariectomized rat faeces.
34044918	4	54	theme	metabolomics	576:587	arg1	analysis					589:596	metabolomics analysis	576:596	metabolomics analysis	576:596	16S rRNA sequencing and metabolomics analysis indicated CSCa intervention altered the composition of gut microbiota along with metabolite profiles in ovariectomized rat faeces.
34805251	9	0	theme	maturational	1758:1769	arg1	changes					1771:1777	positive maturational changes	1749:1777	positive maturational changes	1749:1777	Overall, this study suggests that oral consumption of YBG beginning at pre-diabetic juvenile ages could have positive maturational changes to gut microbiota and immune functions and could result in a delay in the disease onset in those who are pre-disposed to T1D.
34805251	6	1	theme	biosynthesis	1177:1188	arg1	pathways					1190:1197	glycan biosynthesis and metabolism, energy metabolism, and fatty acid biosynthesis pathways	1107:1197	glycan biosynthesis and metabolism, energy metabolism, and fatty acid biosynthesis pathways	1107:1197	Predictive functional analysis, based on 16S rDNA sequences, revealed overrepresentation of glycan biosynthesis and metabolism, energy metabolism, and fatty acid biosynthesis pathways in mice that received YBG for 30 days.
34805251	2	2	theme	adult	333:337	arg1	mice					339:342	adult mice	333:342	adult mice	333:342	We reported before that oral administration of Yeast β-glucan (YBG) in adult mice can help delay type 1 diabetes (T1D) onset and suppress gut inflammation through modulation of the structure and function of gut microbiota.
34805251	6	3	theme	fatty	1166:1170	arg1	biosynthesis					1177:1188	fatty acid biosynthesis	1166:1188	fatty acid biosynthesis	1166:1188	Predictive functional analysis, based on 16S rDNA sequences, revealed overrepresentation of glycan biosynthesis and metabolism, energy metabolism, and fatty acid biosynthesis pathways in mice that received YBG for 30 days.
34805251	4	4	from	incidence	847:855	arg1	microbiota					803:812	gut microbiota	799:812	gut microbiota	799:812	Juvenile mice that received daily oral administration of YBG starting at 15 days of age for 7 or 30 days were examined for changes in gut microbiota, immune characteristics, and T1D incidence.
34805251	7	5	from	increases	1403:1411	arg1	cytokines					1438:1446	some pro-inflammatory cytokines	1416:1446	some pro-inflammatory cytokines (IL-1b, IFN-γ)	1416:1461	Immune phenotype of the colon showed skewing toward immune regulatory and Th17 cytokines with increases in IL-10, IL-17, and IL-21 and a decrease in TNF-α, although increases in some pro-inflammatory cytokines (IL-1b, IFN-γ) were observed.
34805251	7	6	theme	Th17	1312:1315	arg1	cytokines					1317:1325	immune regulatory and Th17 cytokines	1290:1325	immune regulatory and Th17 cytokines	1290:1325	Immune phenotype of the colon showed skewing toward immune regulatory and Th17 cytokines with increases in IL-10, IL-17, and IL-21 and a decrease in TNF-α, although increases in some pro-inflammatory cytokines (IL-1b, IFN-γ) were observed.
34805251	9	7	theme	oral	1674:1677	arg1	consumption					1679:1689	oral consumption	1674:1689	oral consumption of YBG beginning at pre-diabetic juvenile ages	1674:1736	Overall, this study suggests that oral consumption of YBG beginning at pre-diabetic juvenile ages could have positive maturational changes to gut microbiota and immune functions and could result in a delay in the disease onset in those who are pre-disposed to T1D.
34805251	1	8	theme	Complex	156:162	arg1	polysaccharides					172:186	Complex dietary polysaccharides	156:186	Complex dietary polysaccharides such as β-glucans	156:204	Complex dietary polysaccharides such as β-glucans are widely used for their anti-inflammatory properties.
34805251	1	8	theme	Complex	156:162	arg1	β-glucans					196:204	β-glucans	196:204	β-glucans	196:204	Complex dietary polysaccharides such as β-glucans are widely used for their anti-inflammatory properties.
34805251	7	9	theme	regulatory	1297:1306	arg1	cytokines					1317:1325	immune regulatory and Th17 cytokines	1290:1325	immune regulatory and Th17 cytokines	1290:1325	Immune phenotype of the colon showed skewing toward immune regulatory and Th17 cytokines with increases in IL-10, IL-17, and IL-21 and a decrease in TNF-α, although increases in some pro-inflammatory cytokines (IL-1b, IFN-γ) were observed.
34805251	9	10	theme	immune	1801:1806	arg1	functions					1808:1816	immune functions	1801:1816	immune functions	1801:1816	Overall, this study suggests that oral consumption of YBG beginning at pre-diabetic juvenile ages could have positive maturational changes to gut microbiota and immune functions and could result in a delay in the disease onset in those who are pre-disposed to T1D.
34805251	6	11	theme	energy	1143:1148	arg1	metabolism					1150:1159	energy metabolism	1143:1159	energy metabolism	1143:1159	Predictive functional analysis, based on 16S rDNA sequences, revealed overrepresentation of glycan biosynthesis and metabolism, energy metabolism, and fatty acid biosynthesis pathways in mice that received YBG for 30 days.
34805251	2	12	theme	Yeast	309:313	arg1	YBG					325:327	YBG	325:327	YBG	325:327	We reported before that oral administration of Yeast β-glucan (YBG) in adult mice can help delay type 1 diabetes (T1D) onset and suppress gut inflammation through modulation of the structure and function of gut microbiota.
34805251	2	12	theme	Yeast	309:313	arg1	β-glucan					315:322	Yeast β-glucan	309:322	Yeast β-glucan (YBG)	309:328	We reported before that oral administration of Yeast β-glucan (YBG) in adult mice can help delay type 1 diabetes (T1D) onset and suppress gut inflammation through modulation of the structure and function of gut microbiota.
34805251	7	13	theme	colon	1262:1266	arg1	phenotype					1245:1253	Immune phenotype	1238:1253	Immune phenotype of the colon	1238:1266	Immune phenotype of the colon showed skewing toward immune regulatory and Th17 cytokines with increases in IL-10, IL-17, and IL-21 and a decrease in TNF-α, although increases in some pro-inflammatory cytokines (IL-1b, IFN-γ) were observed.
34805251	0	14	theme	Type	106:109	arg1	Onset					122:126	the Eventual Type 1 Diabetes Onset	93:126	the Eventual Type 1 Diabetes Onset	93:126	Impact of Prebiotic β-glucan Treatment at Juvenile Age on the Gut Microbiota Composition and the Eventual Type 1 Diabetes Onset in Non-obese Diabetic Mice.
34805251	4	15	theme	T1D	843:845	arg1	incidence					847:855	T1D incidence	843:855	T1D incidence	843:855	Juvenile mice that received daily oral administration of YBG starting at 15 days of age for 7 or 30 days were examined for changes in gut microbiota, immune characteristics, and T1D incidence.
34805251	0	16	theme	Diabetes	113:120	arg1	Onset					122:126	the Eventual Type 1 Diabetes Onset	93:126	the Eventual Type 1 Diabetes Onset	93:126	Impact of Prebiotic β-glucan Treatment at Juvenile Age on the Gut Microbiota Composition and the Eventual Type 1 Diabetes Onset in Non-obese Diabetic Mice.
34805251	8	17	dep	showed	1541:1546	arg1	suppressed					1562:1571	suppressed	1562:1571	suppressed insulitis	1562:1581	Most importantly, mice that received YBG treatment for 30 days showed significantly suppressed insulitis and delayed onset of hyperglycemia compared to controls.
34805251	8	17	dep	showed	1541:1546	arg1	delayed					1587:1593	delayed	1587:1593	delayed onset of hyperglycemia compared to controls	1587:1637	Most importantly, mice that received YBG treatment for 30 days showed significantly suppressed insulitis and delayed onset of hyperglycemia compared to controls.
34805251	4	18	theme	immune	815:820	arg1	characteristics					822:836	immune characteristics	815:836	immune characteristics	815:836	Juvenile mice that received daily oral administration of YBG starting at 15 days of age for 7 or 30 days were examined for changes in gut microbiota, immune characteristics, and T1D incidence.
34805251	6	19	theme	glycan	1107:1112	arg1	biosynthesis					1114:1125	glycan biosynthesis	1107:1125	glycan biosynthesis	1107:1125	Predictive functional analysis, based on 16S rDNA sequences, revealed overrepresentation of glycan biosynthesis and metabolism, energy metabolism, and fatty acid biosynthesis pathways in mice that received YBG for 30 days.
34805251	4	20	theme	Juvenile	665:672	arg1	mice					674:677	Juvenile mice	665:677	Juvenile mice that received daily oral administration of YBG starting at 15 days of age for 7 or 30 days	665:768	Juvenile mice that received daily oral administration of YBG starting at 15 days of age for 7 or 30 days were examined for changes in gut microbiota, immune characteristics, and T1D incidence.
34805251	0	21	theme	Diabetic	141:148	arg1	Mice					150:153	Non-obese Diabetic Mice	131:153	Non-obese Diabetic Mice	131:153	Impact of Prebiotic β-glucan Treatment at Juvenile Age on the Gut Microbiota Composition and the Eventual Type 1 Diabetes Onset in Non-obese Diabetic Mice.
34805251	7	22	dep	cytokines	1438:1446	arg1	IFN-γ					1456:1460	IFN-γ	1456:1460	IFN-γ	1456:1460	Immune phenotype of the colon showed skewing toward immune regulatory and Th17 cytokines with increases in IL-10, IL-17, and IL-21 and a decrease in TNF-α, although increases in some pro-inflammatory cytokines (IL-1b, IFN-γ) were observed.
34805251	7	22	dep	cytokines	1438:1446	arg1	IL-1b					1449:1453	IL-1b	1449:1453	IL-1b	1449:1453	Immune phenotype of the colon showed skewing toward immune regulatory and Th17 cytokines with increases in IL-10, IL-17, and IL-21 and a decrease in TNF-α, although increases in some pro-inflammatory cytokines (IL-1b, IFN-γ) were observed.
34805251	0	23	from	Composition	77:87	arg1	Mice					150:153	Non-obese Diabetic Mice	131:153	Non-obese Diabetic Mice	131:153	Impact of Prebiotic β-glucan Treatment at Juvenile Age on the Gut Microbiota Composition and the Eventual Type 1 Diabetes Onset in Non-obese Diabetic Mice.
34805251	9	24	theme	disease	1853:1859	arg1	onset					1861:1865	the disease onset	1849:1865	the disease onset	1849:1865	Overall, this study suggests that oral consumption of YBG beginning at pre-diabetic juvenile ages could have positive maturational changes to gut microbiota and immune functions and could result in a delay in the disease onset in those who are pre-disposed to T1D.
34805251	0	25	theme	Gut	62:64	arg1	Composition					77:87	Gut Microbiota Composition	62:87	Gut Microbiota Composition	62:87	Impact of Prebiotic β-glucan Treatment at Juvenile Age on the Gut Microbiota Composition and the Eventual Type 1 Diabetes Onset in Non-obese Diabetic Mice.
34805251	0	26	from	Impact	0:5	arg1	Onset					122:126	the Eventual Type 1 Diabetes Onset	93:126	the Eventual Type 1 Diabetes Onset	93:126	Impact of Prebiotic β-glucan Treatment at Juvenile Age on the Gut Microbiota Composition and the Eventual Type 1 Diabetes Onset in Non-obese Diabetic Mice.
34805251	0	26	from	Impact	0:5	arg1	Composition					77:87	Gut Microbiota Composition	62:87	Gut Microbiota Composition	62:87	Impact of Prebiotic β-glucan Treatment at Juvenile Age on the Gut Microbiota Composition and the Eventual Type 1 Diabetes Onset in Non-obese Diabetic Mice.
34805251	0	26	from	Impact	0:5	arg1	Age					51:53	Juvenile Age	42:53	Juvenile Age	42:53	Impact of Prebiotic β-glucan Treatment at Juvenile Age on the Gut Microbiota Composition and the Eventual Type 1 Diabetes Onset in Non-obese Diabetic Mice.
34805251	5	27	theme	microbiota	979:988	arg1	diversity					960:968	diversity	960:968	diversity	960:968	Mice that received YBG for 30 days but not 7 days, showed considerable changes in the composition and diversity of fecal microbiota as compared to controls.
34805251	5	27	theme	microbiota	979:988	arg1	composition					944:954	composition	944:954	composition	944:954	Mice that received YBG for 30 days but not 7 days, showed considerable changes in the composition and diversity of fecal microbiota as compared to controls.
34805251	7	28	theme	pro-inflammatory	1421:1436	arg1	cytokines					1438:1446	some pro-inflammatory cytokines	1416:1446	some pro-inflammatory cytokines (IL-1b, IFN-γ)	1416:1461	Immune phenotype of the colon showed skewing toward immune regulatory and Th17 cytokines with increases in IL-10, IL-17, and IL-21 and a decrease in TNF-α, although increases in some pro-inflammatory cytokines (IL-1b, IFN-γ) were observed.
34805251	2	29	theme	microbiota	473:482	arg1	function					457:464	function	457:464	function	457:464	We reported before that oral administration of Yeast β-glucan (YBG) in adult mice can help delay type 1 diabetes (T1D) onset and suppress gut inflammation through modulation of the structure and function of gut microbiota.
34805251	2	29	theme	microbiota	473:482	arg1	structure					443:451	structure	443:451	structure	443:451	We reported before that oral administration of Yeast β-glucan (YBG) in adult mice can help delay type 1 diabetes (T1D) onset and suppress gut inflammation through modulation of the structure and function of gut microbiota.
34805251	4	30	theme	YBG	722:724	arg1	administration					704:717	daily oral administration	693:717	daily oral administration of YBG	693:724	Juvenile mice that received daily oral administration of YBG starting at 15 days of age for 7 or 30 days were examined for changes in gut microbiota, immune characteristics, and T1D incidence.
34805251	2	31	theme	diabetes	366:373	arg1	onset					381:385	delay type 1 diabetes (T1D) onset	353:385	delay type 1 diabetes (T1D) onset	353:385	We reported before that oral administration of Yeast β-glucan (YBG) in adult mice can help delay type 1 diabetes (T1D) onset and suppress gut inflammation through modulation of the structure and function of gut microbiota.
34805251	0	32	dep	Composition	77:87	arg1	the					58:60	the	58:60	the	58:60	Impact of Prebiotic β-glucan Treatment at Juvenile Age on the Gut Microbiota Composition and the Eventual Type 1 Diabetes Onset in Non-obese Diabetic Mice.
34805251	2	33	theme	type	359:362	arg1	T1D					376:378	T1D	376:378	T1D	376:378	We reported before that oral administration of Yeast β-glucan (YBG) in adult mice can help delay type 1 diabetes (T1D) onset and suppress gut inflammation through modulation of the structure and function of gut microbiota.
34805251	2	33	theme	type	359:362	arg1	diabetes					366:373	delay type 1 diabetes	353:373	delay type 1 diabetes (T1D) onset	353:385	We reported before that oral administration of Yeast β-glucan (YBG) in adult mice can help delay type 1 diabetes (T1D) onset and suppress gut inflammation through modulation of the structure and function of gut microbiota.
34805251	4	34	theme	daily	693:697	arg1	administration					704:717	daily oral administration	693:717	daily oral administration of YBG	693:724	Juvenile mice that received daily oral administration of YBG starting at 15 days of age for 7 or 30 days were examined for changes in gut microbiota, immune characteristics, and T1D incidence.
34805251	0	35	theme	Prebiotic	10:18	arg1	Treatment					29:37	Prebiotic β-glucan Treatment	10:37	Prebiotic β-glucan Treatment at Juvenile Age	10:53	Impact of Prebiotic β-glucan Treatment at Juvenile Age on the Gut Microbiota Composition and the Eventual Type 1 Diabetes Onset in Non-obese Diabetic Mice.
34805251	6	36	theme	rDNA	1060:1063	arg1	sequences					1065:1073	16S rDNA sequences	1056:1073	16S rDNA sequences	1056:1073	Predictive functional analysis, based on 16S rDNA sequences, revealed overrepresentation of glycan biosynthesis and metabolism, energy metabolism, and fatty acid biosynthesis pathways in mice that received YBG for 30 days.
34805251	9	37	theme	YBG	1694:1696	arg1	consumption					1679:1689	oral consumption	1674:1689	oral consumption of YBG beginning at pre-diabetic juvenile ages	1674:1736	Overall, this study suggests that oral consumption of YBG beginning at pre-diabetic juvenile ages could have positive maturational changes to gut microbiota and immune functions and could result in a delay in the disease onset in those who are pre-disposed to T1D.
34805251	4	38	theme	age	749:751	arg1	days					741:744	15 days	738:744	15 days of age	738:751	Juvenile mice that received daily oral administration of YBG starting at 15 days of age for 7 or 30 days were examined for changes in gut microbiota, immune characteristics, and T1D incidence.
34805251	0	39	theme	Treatment	29:37	arg1	Impact					0:5	Impact	0:5	Impact of Prebiotic β-glucan Treatment at Juvenile Age on the Gut Microbiota Composition and the Eventual Type 1 Diabetes Onset in Non-obese Diabetic Mice.	0:154	Impact of Prebiotic β-glucan Treatment at Juvenile Age on the Gut Microbiota Composition and the Eventual Type 1 Diabetes Onset in Non-obese Diabetic Mice.
34805251	3	40	theme	treatment	600:608	arg1	impact					585:590	the impact	581:590	the impact of oral treatment with YBG in non-obese diabetic (NOD) mice	581:650	Since juvenile age is characterized by profoundly changing immature gut microbiota, we examined the impact of oral treatment with YBG in non-obese diabetic (NOD) mice at this age.
34805251	0	41	theme	Juvenile	42:49	arg1	Age					51:53	Juvenile Age	42:53	Juvenile Age	42:53	Impact of Prebiotic β-glucan Treatment at Juvenile Age on the Gut Microbiota Composition and the Eventual Type 1 Diabetes Onset in Non-obese Diabetic Mice.
34805251	2	42	dep	structure	443:451	arg1	the					439:441	the	439:441	the	439:441	We reported before that oral administration of Yeast β-glucan (YBG) in adult mice can help delay type 1 diabetes (T1D) onset and suppress gut inflammation through modulation of the structure and function of gut microbiota.
34805251	5	43	theme	considerable	916:927	arg1	changes					929:935	considerable changes	916:935	considerable changes in the composition and diversity of fecal microbiota	916:988	Mice that received YBG for 30 days but not 7 days, showed considerable changes in the composition and diversity of fecal microbiota as compared to controls.
34805251	6	44	theme	Predictive	1015:1024	arg1	analysis					1037:1044	Predictive functional analysis	1015:1044	Predictive functional analysis	1015:1044	Predictive functional analysis, based on 16S rDNA sequences, revealed overrepresentation of glycan biosynthesis and metabolism, energy metabolism, and fatty acid biosynthesis pathways in mice that received YBG for 30 days.
34805251	9	45	theme	juvenile	1724:1731	arg1	ages					1733:1736	pre-diabetic juvenile ages	1711:1736	pre-diabetic juvenile ages	1711:1736	Overall, this study suggests that oral consumption of YBG beginning at pre-diabetic juvenile ages could have positive maturational changes to gut microbiota and immune functions and could result in a delay in the disease onset in those who are pre-disposed to T1D.
34805251	1	46	used	used	217:220	arg2	β-glucans					196:204	β-glucans	196:204	β-glucans	196:204	Complex dietary polysaccharides such as β-glucans are widely used for their anti-inflammatory properties.
34805251	1	46	used	used	217:220	arg2	polysaccharides					172:186	Complex dietary polysaccharides	156:186	Complex dietary polysaccharides such as β-glucans	156:204	Complex dietary polysaccharides such as β-glucans are widely used for their anti-inflammatory properties.
34805251	7	47	from	decrease	1375:1382	arg1	TNF-α					1387:1391	TNF-α	1387:1391	TNF-α	1387:1391	Immune phenotype of the colon showed skewing toward immune regulatory and Th17 cytokines with increases in IL-10, IL-17, and IL-21 and a decrease in TNF-α, although increases in some pro-inflammatory cytokines (IL-1b, IFN-γ) were observed.
34805251	7	47	from	decrease	1375:1382	arg1	IL-17					1352:1356	IL-17	1352:1356	IL-17	1352:1356	Immune phenotype of the colon showed skewing toward immune regulatory and Th17 cytokines with increases in IL-10, IL-17, and IL-21 and a decrease in TNF-α, although increases in some pro-inflammatory cytokines (IL-1b, IFN-γ) were observed.
34805251	7	47	from	decrease	1375:1382	arg1	IL-21					1363:1367	IL-21	1363:1367	IL-21	1363:1367	Immune phenotype of the colon showed skewing toward immune regulatory and Th17 cytokines with increases in IL-10, IL-17, and IL-21 and a decrease in TNF-α, although increases in some pro-inflammatory cytokines (IL-1b, IFN-γ) were observed.
34805251	7	47	from	decrease	1375:1382	arg1	IL-10					1345:1349	IL-10	1345:1349	IL-10	1345:1349	Immune phenotype of the colon showed skewing toward immune regulatory and Th17 cytokines with increases in IL-10, IL-17, and IL-21 and a decrease in TNF-α, although increases in some pro-inflammatory cytokines (IL-1b, IFN-γ) were observed.
34805251	9	48	theme	positive	1749:1756	arg1	changes					1771:1777	positive maturational changes	1749:1777	positive maturational changes	1749:1777	Overall, this study suggests that oral consumption of YBG beginning at pre-diabetic juvenile ages could have positive maturational changes to gut microbiota and immune functions and could result in a delay in the disease onset in those who are pre-disposed to T1D.
34805251	5	49	from	changes	929:935	arg1	diversity					960:968	diversity	960:968	diversity	960:968	Mice that received YBG for 30 days but not 7 days, showed considerable changes in the composition and diversity of fecal microbiota as compared to controls.
34805251	5	49	from	changes	929:935	arg1	composition					944:954	composition	944:954	composition	944:954	Mice that received YBG for 30 days but not 7 days, showed considerable changes in the composition and diversity of fecal microbiota as compared to controls.
34805251	6	50	theme	pathways	1190:1197	arg1	overrepresentation					1085:1102	overrepresentation	1085:1102	overrepresentation of glycan biosynthesis and metabolism, energy metabolism, and fatty acid biosynthesis pathways in mice that received YBG for 30 days	1085:1235	Predictive functional analysis, based on 16S rDNA sequences, revealed overrepresentation of glycan biosynthesis and metabolism, energy metabolism, and fatty acid biosynthesis pathways in mice that received YBG for 30 days.
34805251	1	51	theme	anti-inflammatory	232:248	arg1	properties					250:259	their anti-inflammatory properties	226:259	their anti-inflammatory properties	226:259	Complex dietary polysaccharides such as β-glucans are widely used for their anti-inflammatory properties.
34805251	5	52	dep	composition	944:954	arg1	the					940:942	the	940:942	the	940:942	Mice that received YBG for 30 days but not 7 days, showed considerable changes in the composition and diversity of fecal microbiota as compared to controls.
34805251	6	53	theme	acid	1172:1175	arg1	biosynthesis					1177:1188	fatty acid biosynthesis	1166:1188	fatty acid biosynthesis	1166:1188	Predictive functional analysis, based on 16S rDNA sequences, revealed overrepresentation of glycan biosynthesis and metabolism, energy metabolism, and fatty acid biosynthesis pathways in mice that received YBG for 30 days.
34805251	2	54	theme	oral	286:289	arg1	administration					291:304	that oral administration	281:304	that oral administration of Yeast β-glucan (YBG) in adult mice	281:342	We reported before that oral administration of Yeast β-glucan (YBG) in adult mice can help delay type 1 diabetes (T1D) onset and suppress gut inflammation through modulation of the structure and function of gut microbiota.
34805251	9	55	theme	gut	1782:1784	arg1	microbiota					1786:1795	gut microbiota	1782:1795	gut microbiota	1782:1795	Overall, this study suggests that oral consumption of YBG beginning at pre-diabetic juvenile ages could have positive maturational changes to gut microbiota and immune functions and could result in a delay in the disease onset in those who are pre-disposed to T1D.
34805251	3	56	theme	juvenile	491:498	arg1	age					500:502	juvenile age	491:502	juvenile age	491:502	Since juvenile age is characterized by profoundly changing immature gut microbiota, we examined the impact of oral treatment with YBG in non-obese diabetic (NOD) mice at this age.
34805251	4	57	from	characteristics	822:836	arg1	microbiota					803:812	gut microbiota	799:812	gut microbiota	799:812	Juvenile mice that received daily oral administration of YBG starting at 15 days of age for 7 or 30 days were examined for changes in gut microbiota, immune characteristics, and T1D incidence.
34805251	6	58	theme	16S	1056:1058	arg1	sequences					1065:1073	16S rDNA sequences	1056:1073	16S rDNA sequences	1056:1073	Predictive functional analysis, based on 16S rDNA sequences, revealed overrepresentation of glycan biosynthesis and metabolism, energy metabolism, and fatty acid biosynthesis pathways in mice that received YBG for 30 days.
34805251	6	59	theme	metabolism	1150:1159	arg1	pathways					1190:1197	glycan biosynthesis and metabolism, energy metabolism, and fatty acid biosynthesis pathways	1107:1197	glycan biosynthesis and metabolism, energy metabolism, and fatty acid biosynthesis pathways	1107:1197	Predictive functional analysis, based on 16S rDNA sequences, revealed overrepresentation of glycan biosynthesis and metabolism, energy metabolism, and fatty acid biosynthesis pathways in mice that received YBG for 30 days.
34805251	2	60	theme	β-glucan	315:322	arg1	administration					291:304	that oral administration	281:304	that oral administration of Yeast β-glucan (YBG) in adult mice	281:342	We reported before that oral administration of Yeast β-glucan (YBG) in adult mice can help delay type 1 diabetes (T1D) onset and suppress gut inflammation through modulation of the structure and function of gut microbiota.
34805251	1	61	theme	dietary	164:170	arg1	polysaccharides					172:186	Complex dietary polysaccharides	156:186	Complex dietary polysaccharides such as β-glucans	156:204	Complex dietary polysaccharides such as β-glucans are widely used for their anti-inflammatory properties.
34805251	1	61	theme	dietary	164:170	arg1	β-glucans					196:204	β-glucans	196:204	β-glucans	196:204	Complex dietary polysaccharides such as β-glucans are widely used for their anti-inflammatory properties.
34805251	3	62	with	treatment	600:608	arg1	YBG					615:617	YBG	615:617	YBG	615:617	Since juvenile age is characterized by profoundly changing immature gut microbiota, we examined the impact of oral treatment with YBG in non-obese diabetic (NOD) mice at this age.
34805251	3	63	theme	immature	544:551	arg1	microbiota					557:566	immature gut microbiota	544:566	immature gut microbiota	544:566	Since juvenile age is characterized by profoundly changing immature gut microbiota, we examined the impact of oral treatment with YBG in non-obese diabetic (NOD) mice at this age.
34805251	8	64	theme	hyperglycemia	1604:1616	arg1	onset					1595:1599	onset	1595:1599	onset of hyperglycemia	1595:1616	Most importantly, mice that received YBG treatment for 30 days showed significantly suppressed insulitis and delayed onset of hyperglycemia compared to controls.
34805251	6	65	theme	metabolism	1131:1140	arg1	pathways					1190:1197	glycan biosynthesis and metabolism, energy metabolism, and fatty acid biosynthesis pathways	1107:1197	glycan biosynthesis and metabolism, energy metabolism, and fatty acid biosynthesis pathways	1107:1197	Predictive functional analysis, based on 16S rDNA sequences, revealed overrepresentation of glycan biosynthesis and metabolism, energy metabolism, and fatty acid biosynthesis pathways in mice that received YBG for 30 days.
34805251	0	66	theme	Non-obese	131:139	arg1	Mice					150:153	Non-obese Diabetic Mice	131:153	Non-obese Diabetic Mice	131:153	Impact of Prebiotic β-glucan Treatment at Juvenile Age on the Gut Microbiota Composition and the Eventual Type 1 Diabetes Onset in Non-obese Diabetic Mice.
34805251	5	67	theme	fecal	973:977	arg1	microbiota					979:988	fecal microbiota	973:988	fecal microbiota	973:988	Mice that received YBG for 30 days but not 7 days, showed considerable changes in the composition and diversity of fecal microbiota as compared to controls.
34805251	3	68	theme	gut	553:555	arg1	microbiota					557:566	immature gut microbiota	544:566	immature gut microbiota	544:566	Since juvenile age is characterized by profoundly changing immature gut microbiota, we examined the impact of oral treatment with YBG in non-obese diabetic (NOD) mice at this age.
34805251	4	69	theme	gut	799:801	arg1	microbiota					803:812	gut microbiota	799:812	gut microbiota	799:812	Juvenile mice that received daily oral administration of YBG starting at 15 days of age for 7 or 30 days were examined for changes in gut microbiota, immune characteristics, and T1D incidence.
34805251	6	70	theme	biosynthesis	1114:1125	arg1	pathways					1190:1197	glycan biosynthesis and metabolism, energy metabolism, and fatty acid biosynthesis pathways	1107:1197	glycan biosynthesis and metabolism, energy metabolism, and fatty acid biosynthesis pathways	1107:1197	Predictive functional analysis, based on 16S rDNA sequences, revealed overrepresentation of glycan biosynthesis and metabolism, energy metabolism, and fatty acid biosynthesis pathways in mice that received YBG for 30 days.
34805251	2	71	theme	function	457:464	arg1	modulation					425:434	modulation	425:434	modulation of the structure and function of gut microbiota	425:482	We reported before that oral administration of Yeast β-glucan (YBG) in adult mice can help delay type 1 diabetes (T1D) onset and suppress gut inflammation through modulation of the structure and function of gut microbiota.
34805251	7	72	theme	Immune	1238:1243	arg1	phenotype					1245:1253	Immune phenotype	1238:1253	Immune phenotype of the colon	1238:1266	Immune phenotype of the colon showed skewing toward immune regulatory and Th17 cytokines with increases in IL-10, IL-17, and IL-21 and a decrease in TNF-α, although increases in some pro-inflammatory cytokines (IL-1b, IFN-γ) were observed.
34805251	6	73	from	overrepresentation	1085:1102	arg1	mice					1202:1205	mice	1202:1205	mice that received YBG for 30 days	1202:1235	Predictive functional analysis, based on 16S rDNA sequences, revealed overrepresentation of glycan biosynthesis and metabolism, energy metabolism, and fatty acid biosynthesis pathways in mice that received YBG for 30 days.
34805251	0	74	theme	Microbiota	66:75	arg1	Composition					77:87	Gut Microbiota Composition	62:87	Gut Microbiota Composition	62:87	Impact of Prebiotic β-glucan Treatment at Juvenile Age on the Gut Microbiota Composition and the Eventual Type 1 Diabetes Onset in Non-obese Diabetic Mice.
34805251	4	75	from	changes	788:794	arg1	microbiota					803:812	gut microbiota	799:812	gut microbiota	799:812	Juvenile mice that received daily oral administration of YBG starting at 15 days of age for 7 or 30 days were examined for changes in gut microbiota, immune characteristics, and T1D incidence.
34805251	2	76	from	administration	291:304	arg1	mice					339:342	adult mice	333:342	adult mice	333:342	We reported before that oral administration of Yeast β-glucan (YBG) in adult mice can help delay type 1 diabetes (T1D) onset and suppress gut inflammation through modulation of the structure and function of gut microbiota.
34805251	2	77	theme	structure	443:451	arg1	modulation					425:434	modulation	425:434	modulation of the structure and function of gut microbiota	425:482	We reported before that oral administration of Yeast β-glucan (YBG) in adult mice can help delay type 1 diabetes (T1D) onset and suppress gut inflammation through modulation of the structure and function of gut microbiota.
34805251	7	78	from	increases	1332:1340	arg1	TNF-α					1387:1391	TNF-α	1387:1391	TNF-α	1387:1391	Immune phenotype of the colon showed skewing toward immune regulatory and Th17 cytokines with increases in IL-10, IL-17, and IL-21 and a decrease in TNF-α, although increases in some pro-inflammatory cytokines (IL-1b, IFN-γ) were observed.
34805251	7	78	from	increases	1332:1340	arg1	IL-17					1352:1356	IL-17	1352:1356	IL-17	1352:1356	Immune phenotype of the colon showed skewing toward immune regulatory and Th17 cytokines with increases in IL-10, IL-17, and IL-21 and a decrease in TNF-α, although increases in some pro-inflammatory cytokines (IL-1b, IFN-γ) were observed.
34805251	7	78	from	increases	1332:1340	arg1	IL-21					1363:1367	IL-21	1363:1367	IL-21	1363:1367	Immune phenotype of the colon showed skewing toward immune regulatory and Th17 cytokines with increases in IL-10, IL-17, and IL-21 and a decrease in TNF-α, although increases in some pro-inflammatory cytokines (IL-1b, IFN-γ) were observed.
34805251	7	78	from	increases	1332:1340	arg1	IL-10					1345:1349	IL-10	1345:1349	IL-10	1345:1349	Immune phenotype of the colon showed skewing toward immune regulatory and Th17 cytokines with increases in IL-10, IL-17, and IL-21 and a decrease in TNF-α, although increases in some pro-inflammatory cytokines (IL-1b, IFN-γ) were observed.
34805251	0	79	from	Age	51:53	arg1	Impact					0:5	Impact	0:5	Impact of Prebiotic β-glucan Treatment at Juvenile Age on the Gut Microbiota Composition and the Eventual Type 1 Diabetes Onset in Non-obese Diabetic Mice.	0:154	Impact of Prebiotic β-glucan Treatment at Juvenile Age on the Gut Microbiota Composition and the Eventual Type 1 Diabetes Onset in Non-obese Diabetic Mice.
34805251	0	79	from	Age	51:53	arg1	Treatment					29:37	Prebiotic β-glucan Treatment	10:37	Prebiotic β-glucan Treatment at Juvenile Age	10:53	Impact of Prebiotic β-glucan Treatment at Juvenile Age on the Gut Microbiota Composition and the Eventual Type 1 Diabetes Onset in Non-obese Diabetic Mice.
34805251	4	80	theme	oral	699:702	arg1	administration					704:717	daily oral administration	693:717	daily oral administration of YBG	693:724	Juvenile mice that received daily oral administration of YBG starting at 15 days of age for 7 or 30 days were examined for changes in gut microbiota, immune characteristics, and T1D incidence.
34805251	0	81	theme	Eventual	97:104	arg1	Onset					122:126	the Eventual Type 1 Diabetes Onset	93:126	the Eventual Type 1 Diabetes Onset	93:126	Impact of Prebiotic β-glucan Treatment at Juvenile Age on the Gut Microbiota Composition and the Eventual Type 1 Diabetes Onset in Non-obese Diabetic Mice.
34805251	2	82	theme	gut	469:471	arg1	microbiota					473:482	gut microbiota	469:482	gut microbiota	469:482	We reported before that oral administration of Yeast β-glucan (YBG) in adult mice can help delay type 1 diabetes (T1D) onset and suppress gut inflammation through modulation of the structure and function of gut microbiota.
34805251	3	83	from	impact	585:590	arg1	mice					647:650	non-obese diabetic (NOD) mice	622:650	non-obese diabetic (NOD) mice	622:650	Since juvenile age is characterized by profoundly changing immature gut microbiota, we examined the impact of oral treatment with YBG in non-obese diabetic (NOD) mice at this age.
34805251	2	84	theme	gut	400:402	arg1	inflammation					404:415	gut inflammation	400:415	gut inflammation	400:415	We reported before that oral administration of Yeast β-glucan (YBG) in adult mice can help delay type 1 diabetes (T1D) onset and suppress gut inflammation through modulation of the structure and function of gut microbiota.
34805251	2	85	theme	delay	353:357	arg1	T1D					376:378	T1D	376:378	T1D	376:378	We reported before that oral administration of Yeast β-glucan (YBG) in adult mice can help delay type 1 diabetes (T1D) onset and suppress gut inflammation through modulation of the structure and function of gut microbiota.
34805251	2	85	theme	delay	353:357	arg1	diabetes					366:373	delay type 1 diabetes	353:373	delay type 1 diabetes (T1D) onset	353:385	We reported before that oral administration of Yeast β-glucan (YBG) in adult mice can help delay type 1 diabetes (T1D) onset and suppress gut inflammation through modulation of the structure and function of gut microbiota.
34805251	0	86	theme	β-glucan	20:27	arg1	Treatment					29:37	Prebiotic β-glucan Treatment	10:37	Prebiotic β-glucan Treatment at Juvenile Age	10:53	Impact of Prebiotic β-glucan Treatment at Juvenile Age on the Gut Microbiota Composition and the Eventual Type 1 Diabetes Onset in Non-obese Diabetic Mice.
34805251	9	87	from	delay	1840:1844	arg1	onset					1861:1865	the disease onset	1849:1865	the disease onset	1849:1865	Overall, this study suggests that oral consumption of YBG beginning at pre-diabetic juvenile ages could have positive maturational changes to gut microbiota and immune functions and could result in a delay in the disease onset in those who are pre-disposed to T1D.
34805251	9	88	contain	have	1744:1747	arg1	consumption					1679:1689	oral consumption	1674:1689	oral consumption of YBG beginning at pre-diabetic juvenile ages	1674:1736	Overall, this study suggests that oral consumption of YBG beginning at pre-diabetic juvenile ages could have positive maturational changes to gut microbiota and immune functions and could result in a delay in the disease onset in those who are pre-disposed to T1D.
34805251	9	88	contain	have	1744:1747	arg2	changes					1771:1777	positive maturational changes	1749:1777	positive maturational changes	1749:1777	Overall, this study suggests that oral consumption of YBG beginning at pre-diabetic juvenile ages could have positive maturational changes to gut microbiota and immune functions and could result in a delay in the disease onset in those who are pre-disposed to T1D.
34805251	3	89	theme	oral	595:598	arg1	treatment					600:608	oral treatment	595:608	oral treatment with YBG	595:617	Since juvenile age is characterized by profoundly changing immature gut microbiota, we examined the impact of oral treatment with YBG in non-obese diabetic (NOD) mice at this age.
34805251	6	90	theme	functional	1026:1035	arg1	analysis					1037:1044	Predictive functional analysis	1015:1044	Predictive functional analysis	1015:1044	Predictive functional analysis, based on 16S rDNA sequences, revealed overrepresentation of glycan biosynthesis and metabolism, energy metabolism, and fatty acid biosynthesis pathways in mice that received YBG for 30 days.
34805251	9	91	theme	pre-diabetic	1711:1722	arg1	ages					1733:1736	pre-diabetic juvenile ages	1711:1736	pre-diabetic juvenile ages	1711:1736	Overall, this study suggests that oral consumption of YBG beginning at pre-diabetic juvenile ages could have positive maturational changes to gut microbiota and immune functions and could result in a delay in the disease onset in those who are pre-disposed to T1D.
34805251	3	92	theme	diabetic	632:639	arg1	mice					647:650	non-obese diabetic (NOD) mice	622:650	non-obese diabetic (NOD) mice	622:650	Since juvenile age is characterized by profoundly changing immature gut microbiota, we examined the impact of oral treatment with YBG in non-obese diabetic (NOD) mice at this age.
34805251	8	93	theme	YBG	1515:1517	arg1	treatment					1519:1527	YBG treatment	1515:1527	YBG treatment	1515:1527	Most importantly, mice that received YBG treatment for 30 days showed significantly suppressed insulitis and delayed onset of hyperglycemia compared to controls.
34805251	0	94	from	Onset	122:126	arg1	Mice					150:153	Non-obese Diabetic Mice	131:153	Non-obese Diabetic Mice	131:153	Impact of Prebiotic β-glucan Treatment at Juvenile Age on the Gut Microbiota Composition and the Eventual Type 1 Diabetes Onset in Non-obese Diabetic Mice.
33446706	0	0	theme	ionic	94:98	arg1	techniques					109:118	optimized ionic gelation techniques	84:118	optimized ionic gelation techniques	84:118	Modified-release of encapsulated bioactive compounds from annatto seeds produced by optimized ionic gelation techniques.
33446706	4	1	dep	CaCl2	785:789	arg1	whereas					837:843	whereas	837:843	whereas	837:843	The optimized EG conditions were 0.3% CaCl2 and 1:1.2 alginate to gelling solution ratio, whereas a 0.3% CH3COOH and 1:5 alginate to gelling solution ratio were optimized conditions for IG.
33446706	0	2	theme	optimized	84:92	arg1	techniques					109:118	optimized ionic gelation techniques	84:118	optimized ionic gelation techniques	84:118	Modified-release of encapsulated bioactive compounds from annatto seeds produced by optimized ionic gelation techniques.
33446706	4	3	theme	EG	761:762	arg1	conditions					764:773	The optimized EG conditions	747:773	The optimized EG conditions	747:773	The optimized EG conditions were 0.3% CaCl2 and 1:1.2 alginate to gelling solution ratio, whereas a 0.3% CH3COOH and 1:5 alginate to gelling solution ratio were optimized conditions for IG.
33446706	4	3	theme	EG	761:762	arg1	CaCl2					785:789	0.3% CaCl2	780:789	0.3% CaCl2	780:789	The optimized EG conditions were 0.3% CaCl2 and 1:1.2 alginate to gelling solution ratio, whereas a 0.3% CH3COOH and 1:5 alginate to gelling solution ratio were optimized conditions for IG.
33446706	2	4	theme	chloride	312:319	arg1	alginate					452:459	alginate	452:459	alginate to gelling solution ratio (1:2-1:6)	452:495	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	2	4	theme	chloride	312:319	arg1	concentration					329:341	Calcium chloride (CaCl2) concentration	304:341	Calcium chloride (CaCl2) concentration (0.3-3.5%)	304:352	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	2	4	theme	chloride	312:319	arg1	%					351:351	0.3-3.5%	344:351	0.3-3.5%	344:351	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	2	4	theme	chloride	312:319	arg1	variables					523:531	independent variables	511:531	independent variables for EG and IG respectively	511:558	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	2	4	theme	chloride	312:319	arg1	concentration					423:435	alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration	355:435	alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%)	355:446	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	1	5	theme	process	238:244	arg1	yield					246:250	process yield	238:250	process yield (% Y)	238:256	To compare the encapsulation of annatto extract by external gelation (EG) and internal gelation (IG) and to maximize process yield (% Y), two central composite designs were proposed.
33446706	1	5	theme	process	238:244	arg1	Y					255:255	% Y	253:255	% Y	253:255	To compare the encapsulation of annatto extract by external gelation (EG) and internal gelation (IG) and to maximize process yield (% Y), two central composite designs were proposed.
33446706	4	6	theme	0.3	780:782	arg1	%					783:783	%	783:783	%	783:783	The optimized EG conditions were 0.3% CaCl2 and 1:1.2 alginate to gelling solution ratio, whereas a 0.3% CH3COOH and 1:5 alginate to gelling solution ratio were optimized conditions for IG.
33446706	0	7	theme	gelation	100:107	arg1	techniques					109:118	optimized ionic gelation techniques	84:118	optimized ionic gelation techniques	84:118	Modified-release of encapsulated bioactive compounds from annatto seeds produced by optimized ionic gelation techniques.
33446706	0	8	from	seeds	66:70	arg1	Modified-release					0:15	Modified-release	0:15	Modified-release of encapsulated bioactive compounds from annatto seeds	0:70	Modified-release of encapsulated bioactive compounds from annatto seeds produced by optimized ionic gelation techniques.
33446706	4	9	theme	1:5	864:866	arg1	alginate					868:875	1:5 alginate	864:875	1:5 alginate	864:875	The optimized EG conditions were 0.3% CaCl2 and 1:1.2 alginate to gelling solution ratio, whereas a 0.3% CH3COOH and 1:5 alginate to gelling solution ratio were optimized conditions for IG.
33446706	3	10	theme	particle	632:639	arg1	size					641:644	particle size	632:644	particle size	632:644	Release studies were conducted under different conditions; morphology, particle size, the encapsulation efficiency (EE), and release mechanism were evaluated under optimized conditions.
33446706	2	11	theme	alginate	355:362	arg1	alginate					452:459	alginate	452:459	alginate to gelling solution ratio (1:2-1:6)	452:495	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	2	11	theme	alginate	355:362	arg1	concentration					329:341	Calcium chloride (CaCl2) concentration	304:341	Calcium chloride (CaCl2) concentration (0.3-3.5%)	304:352	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	2	11	theme	alginate	355:362	arg1	variables					523:531	independent variables	511:531	independent variables for EG and IG respectively	511:558	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	2	11	theme	alginate	355:362	arg1	concentration					423:435	alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration	355:435	alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%)	355:446	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	2	11	theme	alginate	355:362	arg1	%					445:445	0.2-5.0%	438:445	0.2-5.0%	438:445	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	5	12	theme	highest	972:978	arg1	EE					980:981	the highest EE	968:981	the highest EE	968:981	When 20% extract was employed, the highest EE was achieved, and the largest release was obtained at a pH 6.5 buffer.
33446706	2	13	theme	independent	511:521	arg1	alginate					452:459	alginate	452:459	alginate to gelling solution ratio (1:2-1:6)	452:495	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	2	13	theme	independent	511:521	arg1	concentration					329:341	Calcium chloride (CaCl2) concentration	304:341	Calcium chloride (CaCl2) concentration (0.3-3.5%)	304:352	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	2	13	theme	independent	511:521	arg1	variables					523:531	independent variables	511:531	independent variables for EG and IG respectively	511:558	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	2	13	theme	independent	511:521	arg1	concentration					423:435	alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration	355:435	alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%)	355:446	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	4	14	theme	solution	888:895	arg1	ratio					897:901	gelling solution ratio	880:901	gelling solution ratio	880:901	The optimized EG conditions were 0.3% CaCl2 and 1:1.2 alginate to gelling solution ratio, whereas a 0.3% CH3COOH and 1:5 alginate to gelling solution ratio were optimized conditions for IG.
33446706	1	15	theme	%	253:253	arg1	yield					246:250	process yield	238:250	process yield (% Y)	238:256	To compare the encapsulation of annatto extract by external gelation (EG) and internal gelation (IG) and to maximize process yield (% Y), two central composite designs were proposed.
33446706	1	15	theme	%	253:253	arg1	Y					255:255	% Y	253:255	% Y	253:255	To compare the encapsulation of annatto extract by external gelation (EG) and internal gelation (IG) and to maximize process yield (% Y), two central composite designs were proposed.
33446706	2	16	dep	ratio	384:388	arg1	CH3COOH					414:420	CH3COOH	414:420	CH3COOH	414:420	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	2	16	dep	ratio	384:388	arg1	acid					408:411	acetic acid	401:411	gelling solution ratio (1:2-1:6); acetic acid (CH3COOH)	367:421	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	7	17	theme	Polyphenol	1123:1132	arg1	release					1134:1140	Polyphenol release	1123:1140	Polyphenol release	1123:1140	Polyphenol release was driven by diffusion, whereas bixin showed anomalous release.
33446706	8	18	theme	food	1282:1285	arg1	matrices					1287:1294	food matrices	1282:1294	food matrices	1282:1294	These results are promising for application as modulated release agents in food matrices.
33446706	2	19	theme	solution	375:382	arg1	ratio					384:388	gelling solution ratio	367:388	gelling solution ratio (1:2-1:6); acetic acid (CH3COOH)	367:421	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	2	19	theme	solution	375:382	arg1	1:2-1:6					391:397	1:2-1:6	391:397	1:2-1:6	391:397	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	3	20	theme	encapsulation	651:663	arg1	EE					677:678	EE	677:678	EE	677:678	Release studies were conducted under different conditions; morphology, particle size, the encapsulation efficiency (EE), and release mechanism were evaluated under optimized conditions.
33446706	3	20	theme	encapsulation	651:663	arg1	efficiency					665:674	the encapsulation efficiency	647:674	the encapsulation efficiency (EE)	647:679	Release studies were conducted under different conditions; morphology, particle size, the encapsulation efficiency (EE), and release mechanism were evaluated under optimized conditions.
33446706	8	21	from	agents	1272:1277	arg1	matrices					1287:1294	food matrices	1282:1294	food matrices	1282:1294	These results are promising for application as modulated release agents in food matrices.
33446706	0	22	theme	encapsulated	20:31	arg1	compounds					43:51	encapsulated bioactive compounds	20:51	encapsulated bioactive compounds	20:51	Modified-release of encapsulated bioactive compounds from annatto seeds produced by optimized ionic gelation techniques.
33446706	4	23	theme	gelling	813:819	arg1	ratio					830:834	gelling solution ratio	813:834	gelling solution ratio	813:834	The optimized EG conditions were 0.3% CaCl2 and 1:1.2 alginate to gelling solution ratio, whereas a 0.3% CH3COOH and 1:5 alginate to gelling solution ratio were optimized conditions for IG.
33446706	1	24	theme	external	172:179	arg1	EG					191:192	EG	191:192	EG	191:192	To compare the encapsulation of annatto extract by external gelation (EG) and internal gelation (IG) and to maximize process yield (% Y), two central composite designs were proposed.
33446706	1	24	theme	external	172:179	arg1	gelation					181:188	external gelation	172:188	external gelation (EG)	172:193	To compare the encapsulation of annatto extract by external gelation (EG) and internal gelation (IG) and to maximize process yield (% Y), two central composite designs were proposed.
33446706	1	25	theme	internal	199:206	arg1	IG					218:219	IG	218:219	IG	218:219	To compare the encapsulation of annatto extract by external gelation (EG) and internal gelation (IG) and to maximize process yield (% Y), two central composite designs were proposed.
33446706	1	25	theme	internal	199:206	arg1	gelation					208:215	internal gelation	199:215	internal gelation (IG)	199:220	To compare the encapsulation of annatto extract by external gelation (EG) and internal gelation (IG) and to maximize process yield (% Y), two central composite designs were proposed.
33446706	2	26	theme	acetic	401:406	arg1	CH3COOH					414:420	CH3COOH	414:420	CH3COOH	414:420	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	2	26	theme	acetic	401:406	arg1	acid					408:411	acetic acid	401:411	gelling solution ratio (1:2-1:6); acetic acid (CH3COOH)	367:421	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	4	27	theme	solution	821:828	arg1	ratio					830:834	gelling solution ratio	813:834	gelling solution ratio	813:834	The optimized EG conditions were 0.3% CaCl2 and 1:1.2 alginate to gelling solution ratio, whereas a 0.3% CH3COOH and 1:5 alginate to gelling solution ratio were optimized conditions for IG.
33446706	5	28	theme	20	942:943	arg1	%					944:944	%	944:944	%	944:944	When 20% extract was employed, the highest EE was achieved, and the largest release was obtained at a pH 6.5 buffer.
33446706	5	29	theme	pH	1039:1040	arg1	buffer					1046:1051	a pH 6.5 buffer	1037:1051	a pH 6.5 buffer	1037:1051	When 20% extract was employed, the highest EE was achieved, and the largest release was obtained at a pH 6.5 buffer.
33446706	0	30	theme	compounds	43:51	arg1	Modified-release					0:15	Modified-release	0:15	Modified-release of encapsulated bioactive compounds from annatto seeds	0:70	Modified-release of encapsulated bioactive compounds from annatto seeds produced by optimized ionic gelation techniques.
33446706	3	31	theme	optimized	725:733	arg1	conditions					735:744	optimized conditions	725:744	optimized conditions	725:744	Release studies were conducted under different conditions; morphology, particle size, the encapsulation efficiency (EE), and release mechanism were evaluated under optimized conditions.
33446706	5	32	theme	%	944:944	arg1	extract					946:952	20% extract	942:952	20% extract	942:952	When 20% extract was employed, the highest EE was achieved, and the largest release was obtained at a pH 6.5 buffer.
33446706	0	33	theme	bioactive	33:41	arg1	compounds					43:51	encapsulated bioactive compounds	20:51	encapsulated bioactive compounds	20:51	Modified-release of encapsulated bioactive compounds from annatto seeds produced by optimized ionic gelation techniques.
33446706	4	34	theme	gelling	880:886	arg1	ratio					897:901	gelling solution ratio	880:901	gelling solution ratio	880:901	The optimized EG conditions were 0.3% CaCl2 and 1:1.2 alginate to gelling solution ratio, whereas a 0.3% CH3COOH and 1:5 alginate to gelling solution ratio were optimized conditions for IG.
33446706	1	35	theme	central	263:269	arg1	designs					281:287	two central composite designs	259:287	two central composite designs	259:287	To compare the encapsulation of annatto extract by external gelation (EG) and internal gelation (IG) and to maximize process yield (% Y), two central composite designs were proposed.
33446706	4	36	theme	1:1.2	795:799	arg1	alginate					801:808	1:1.2 alginate	795:808	1:1.2 alginate	795:808	The optimized EG conditions were 0.3% CaCl2 and 1:1.2 alginate to gelling solution ratio, whereas a 0.3% CH3COOH and 1:5 alginate to gelling solution ratio were optimized conditions for IG.
33446706	2	37	theme	Calcium	304:310	arg1	alginate					452:459	alginate	452:459	alginate to gelling solution ratio (1:2-1:6)	452:495	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	2	37	theme	Calcium	304:310	arg1	concentration					329:341	Calcium chloride (CaCl2) concentration	304:341	Calcium chloride (CaCl2) concentration (0.3-3.5%)	304:352	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	2	37	theme	Calcium	304:310	arg1	%					351:351	0.3-3.5%	344:351	0.3-3.5%	344:351	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	2	37	theme	Calcium	304:310	arg1	variables					523:531	independent variables	511:531	independent variables for EG and IG respectively	511:558	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	2	37	theme	Calcium	304:310	arg1	concentration					423:435	alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration	355:435	alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%)	355:446	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	0	38	theme	annatto	58:64	arg1	seeds					66:70	annatto seeds	58:70	annatto seeds	58:70	Modified-release of encapsulated bioactive compounds from annatto seeds produced by optimized ionic gelation techniques.
33446706	1	39	theme	composite	271:279	arg1	designs					281:287	two central composite designs	259:287	two central composite designs	259:287	To compare the encapsulation of annatto extract by external gelation (EG) and internal gelation (IG) and to maximize process yield (% Y), two central composite designs were proposed.
33446706	4	40	theme	%	783:783	arg1	conditions					764:773	The optimized EG conditions	747:773	The optimized EG conditions	747:773	The optimized EG conditions were 0.3% CaCl2 and 1:1.2 alginate to gelling solution ratio, whereas a 0.3% CH3COOH and 1:5 alginate to gelling solution ratio were optimized conditions for IG.
33446706	4	40	theme	%	783:783	arg1	CaCl2					785:789	0.3% CaCl2	780:789	0.3% CaCl2	780:789	The optimized EG conditions were 0.3% CaCl2 and 1:1.2 alginate to gelling solution ratio, whereas a 0.3% CH3COOH and 1:5 alginate to gelling solution ratio were optimized conditions for IG.
33446706	6	41	theme	best	1092:1095	arg1	fit					1097:1099	the best fit	1088:1099	the best fit to experimental data	1088:1120	The Peppas-Sahlin model presented the best fit to experimental data.
33446706	6	42	theme	experimental	1104:1115	arg1	data					1117:1120	experimental data	1104:1120	experimental data	1104:1120	The Peppas-Sahlin model presented the best fit to experimental data.
33446706	5	43	theme	largest	1005:1011	arg1	release					1013:1019	the largest release	1001:1019	the largest release	1001:1019	When 20% extract was employed, the highest EE was achieved, and the largest release was obtained at a pH 6.5 buffer.
33446706	2	44	theme	solution	472:479	arg1	1:2-1:6					488:494	1:2-1:6	488:494	1:2-1:6	488:494	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	2	44	theme	solution	472:479	arg1	ratio					481:485	gelling solution ratio	464:485	gelling solution ratio (1:2-1:6)	464:495	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	8	45	theme	release	1264:1270	arg1	agents					1272:1277	modulated release agents	1254:1277	modulated release agents in food matrices	1254:1294	These results are promising for application as modulated release agents in food matrices.
33446706	7	46	theme	anomalous	1188:1196	arg1	release					1198:1204	anomalous release	1188:1204	anomalous release	1188:1204	Polyphenol release was driven by diffusion, whereas bixin showed anomalous release.
33446706	6	47	theme	Peppas-Sahlin	1058:1070	arg1	model					1072:1076	The Peppas-Sahlin model	1054:1076	The Peppas-Sahlin model	1054:1076	The Peppas-Sahlin model presented the best fit to experimental data.
33446706	3	48	theme	release	686:692	arg1	mechanism					694:702	release mechanism	686:702	release mechanism	686:702	Release studies were conducted under different conditions; morphology, particle size, the encapsulation efficiency (EE), and release mechanism were evaluated under optimized conditions.
33446706	2	49	theme	gelling	464:470	arg1	1:2-1:6					488:494	1:2-1:6	488:494	1:2-1:6	488:494	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	2	49	theme	gelling	464:470	arg1	ratio					481:485	gelling solution ratio	464:485	gelling solution ratio (1:2-1:6)	464:495	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	4	50	theme	optimized	751:759	arg1	conditions					764:773	The optimized EG conditions	747:773	The optimized EG conditions	747:773	The optimized EG conditions were 0.3% CaCl2 and 1:1.2 alginate to gelling solution ratio, whereas a 0.3% CH3COOH and 1:5 alginate to gelling solution ratio were optimized conditions for IG.
33446706	4	50	theme	optimized	751:759	arg1	CaCl2					785:789	0.3% CaCl2	780:789	0.3% CaCl2	780:789	The optimized EG conditions were 0.3% CaCl2 and 1:1.2 alginate to gelling solution ratio, whereas a 0.3% CH3COOH and 1:5 alginate to gelling solution ratio were optimized conditions for IG.
33446706	8	51	theme	modulated	1254:1262	arg1	agents					1272:1277	modulated release agents	1254:1277	modulated release agents in food matrices	1254:1294	These results are promising for application as modulated release agents in food matrices.
33446706	1	52	theme	extract	161:167	arg1	encapsulation					136:148	the encapsulation	132:148	the encapsulation of annatto extract by external gelation (EG) and internal gelation (IG)	132:220	To compare the encapsulation of annatto extract by external gelation (EG) and internal gelation (IG) and to maximize process yield (% Y), two central composite designs were proposed.
33446706	4	53	theme	%	850:850	arg1	CH3COOH					852:858	a 0.3% CH3COOH	845:858	a 0.3% CH3COOH	845:858	The optimized EG conditions were 0.3% CaCl2 and 1:1.2 alginate to gelling solution ratio, whereas a 0.3% CH3COOH and 1:5 alginate to gelling solution ratio were optimized conditions for IG.
33446706	3	54	theme	Release	561:567	arg1	studies					569:575	Release studies	561:575	Release studies	561:575	Release studies were conducted under different conditions; morphology, particle size, the encapsulation efficiency (EE), and release mechanism were evaluated under optimized conditions.
33446706	1	55	theme	annatto	153:159	arg1	extract					161:167	annatto extract	153:167	annatto extract	153:167	To compare the encapsulation of annatto extract by external gelation (EG) and internal gelation (IG) and to maximize process yield (% Y), two central composite designs were proposed.
33446706	4	56	theme	0.3	847:849	arg1	%					850:850	%	850:850	%	850:850	The optimized EG conditions were 0.3% CaCl2 and 1:1.2 alginate to gelling solution ratio, whereas a 0.3% CH3COOH and 1:5 alginate to gelling solution ratio were optimized conditions for IG.
33446706	3	57	theme	different	598:606	arg1	conditions					608:617	different conditions	598:617	different conditions	598:617	Release studies were conducted under different conditions; morphology, particle size, the encapsulation efficiency (EE), and release mechanism were evaluated under optimized conditions.
33446706	2	58	theme	gelling	367:373	arg1	ratio					384:388	gelling solution ratio	367:388	gelling solution ratio (1:2-1:6); acetic acid (CH3COOH)	367:421	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	2	58	theme	gelling	367:373	arg1	1:2-1:6					391:397	1:2-1:6	391:397	1:2-1:6	391:397	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	2	59	theme	CaCl2	322:326	arg1	alginate					452:459	alginate	452:459	alginate to gelling solution ratio (1:2-1:6)	452:495	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	2	59	theme	CaCl2	322:326	arg1	concentration					329:341	Calcium chloride (CaCl2) concentration	304:341	Calcium chloride (CaCl2) concentration (0.3-3.5%)	304:352	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	2	59	theme	CaCl2	322:326	arg1	%					351:351	0.3-3.5%	344:351	0.3-3.5%	344:351	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	2	59	theme	CaCl2	322:326	arg1	variables					523:531	independent variables	511:531	independent variables for EG and IG respectively	511:558	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
33446706	2	59	theme	CaCl2	322:326	arg1	concentration					423:435	alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration	355:435	alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%)	355:446	Calcium chloride (CaCl2) concentration (0.3-3.5%), alginate to gelling solution ratio (1:2-1:6); acetic acid (CH3COOH) concentration (0.2-5.0%) and alginate to gelling solution ratio (1:2-1:6) were taken as independent variables for EG and IG respectively.
34215912	5	0	from	accumulation	1141:1152	arg1	seeds					1157:1161	seeds	1157:1161	seeds	1157:1161	Here, we generated AIL7 seed-specific over-expression lines and found that they exhibited significant alterations in FA composition and decreased total lipid accumulation in seeds.
34215912	2	1	contain	have	440:443	arg2	applications					477:488	industrial applications	466:488	industrial applications	466:488	Seed fatty acids (FAs) and triacylglycerol (TAG) contribute to many functions in plants, and seed lipids have broad food, feed and industrial applications.
34215912	2	1	contain	have	440:443	arg2	food					451:454	broad food	445:454	broad food	445:454	Seed fatty acids (FAs) and triacylglycerol (TAG) contribute to many functions in plants, and seed lipids have broad food, feed and industrial applications.
34215912	2	1	contain	have	440:443	arg2	feed					457:460	feed	457:460	feed	457:460	Seed fatty acids (FAs) and triacylglycerol (TAG) contribute to many functions in plants, and seed lipids have broad food, feed and industrial applications.
34215912	2	1	contain	have	440:443	arg1	lipids					433:438	seed lipids	428:438	seed lipids	428:438	Seed fatty acids (FAs) and triacylglycerol (TAG) contribute to many functions in plants, and seed lipids have broad food, feed and industrial applications.
34215912	1	2	theme	transcriptional	287:301	arg1	regulation					303:312	the transcriptional regulation	283:312	the transcriptional regulation of associated genes	283:332	AIL7 over-expression modulates fatty acid biosynthesis and triacylglycerol accumulation in Arabidopsis developing seeds through the transcriptional regulation of associated genes.
34215912	9	3	theme	engineering	1922:1932	arg1	endeavors					1934:1942	metabolic engineering endeavors	1912:1942	metabolic engineering endeavors	1912:1942	These findings not only advance our understanding of the lipid biosynthetic pathway in seeds, but also provide evidence for additional functions of AIL7, which could prove valuable in downstream breeding and/or metabolic engineering endeavors.
34215912	4	4	from	effect	915:920	arg1	context					929:935	the context	925:935	the context of lipid biosynthesis	925:957	Although AINTEGUMENTA-LIKE 7 (AIL7) is involved in meristematic function and shoot phyllotaxy, its effect in the context of lipid biosynthesis has yet to be assessed.
34215912	7	5	theme	wild-type	1479:1487	arg1	plants					1489:1494	wild-type plants	1479:1494	wild-type plants	1479:1494	Correspondingly, RNA-Seq analysis demonstrated that the expression of many genes related to FA biosynthesis and TAG breakdown were significantly altered in developing siliques from transgenic lines compared to wild-type plants.
34215912	7	6	theme	developing	1425:1434	arg1	siliques					1436:1443	developing siliques	1425:1443	developing siliques from transgenic lines	1425:1465	Correspondingly, RNA-Seq analysis demonstrated that the expression of many genes related to FA biosynthesis and TAG breakdown were significantly altered in developing siliques from transgenic lines compared to wild-type plants.
34215912	3	7	theme	attention	526:534	arg1	result					496:501	a result	494:501	a result	494:501	As a result, an enormous amount of attention has been dedicated towards uncovering the regulatory cascade responsible for the fine-tuning of the lipid biosynthetic pathway in seeds, which is regulated in part through the action of LEAFY COTYLEDON1, ABSCISSIC ACID INSENSITIVE 3, FUSCA3 and LEC2 (LAFL) transcription factors.
34215912	3	7	theme	attention	526:534	arg1	attention					526:534	attention	526:534	attention	526:534	As a result, an enormous amount of attention has been dedicated towards uncovering the regulatory cascade responsible for the fine-tuning of the lipid biosynthetic pathway in seeds, which is regulated in part through the action of LEAFY COTYLEDON1, ABSCISSIC ACID INSENSITIVE 3, FUSCA3 and LEC2 (LAFL) transcription factors.
34215912	3	7	theme	attention	526:534	arg1	amount					516:521	an enormous amount	504:521	an enormous amount of attention	504:534	As a result, an enormous amount of attention has been dedicated towards uncovering the regulatory cascade responsible for the fine-tuning of the lipid biosynthetic pathway in seeds, which is regulated in part through the action of LEAFY COTYLEDON1, ABSCISSIC ACID INSENSITIVE 3, FUSCA3 and LEC2 (LAFL) transcription factors.
34215912	1	8	theme	fatty	186:190	arg1	biosynthesis					197:208	fatty acid biosynthesis	186:208	fatty acid biosynthesis	186:208	AIL7 over-expression modulates fatty acid biosynthesis and triacylglycerol accumulation in Arabidopsis developing seeds through the transcriptional regulation of associated genes.
34215912	7	9	from	lines	1461:1465	arg1	siliques					1436:1443	developing siliques	1425:1443	developing siliques from transgenic lines	1425:1465	Correspondingly, RNA-Seq analysis demonstrated that the expression of many genes related to FA biosynthesis and TAG breakdown were significantly altered in developing siliques from transgenic lines compared to wild-type plants.
34215912	3	10	theme	ABSCISSIC	740:748	arg1	ACID					750:753	ABSCISSIC ACID INSENSITIVE 3	740:767	ABSCISSIC ACID INSENSITIVE 3	740:767	As a result, an enormous amount of attention has been dedicated towards uncovering the regulatory cascade responsible for the fine-tuning of the lipid biosynthetic pathway in seeds, which is regulated in part through the action of LEAFY COTYLEDON1, ABSCISSIC ACID INSENSITIVE 3, FUSCA3 and LEC2 (LAFL) transcription factors.
34215912	3	11	theme	COTYLEDON1	728:737	arg1	action					712:717	the action	708:717	the action of LEAFY COTYLEDON1, ABSCISSIC ACID INSENSITIVE 3, FUSCA3 and LEC2 (LAFL) transcription factors	708:813	As a result, an enormous amount of attention has been dedicated towards uncovering the regulatory cascade responsible for the fine-tuning of the lipid biosynthetic pathway in seeds, which is regulated in part through the action of LEAFY COTYLEDON1, ABSCISSIC ACID INSENSITIVE 3, FUSCA3 and LEC2 (LAFL) transcription factors.
34215912	2	12	theme	fatty	340:344	arg1	acids					346:350	Seed fatty acids	335:350	Seed fatty acids (FAs)	335:356	Seed fatty acids (FAs) and triacylglycerol (TAG) contribute to many functions in plants, and seed lipids have broad food, feed and industrial applications.
34215912	2	12	theme	fatty	340:344	arg1	FAs					353:355	FAs	353:355	FAs	353:355	Seed fatty acids (FAs) and triacylglycerol (TAG) contribute to many functions in plants, and seed lipids have broad food, feed and industrial applications.
34215912	1	13	theme	triacylglycerol	214:228	arg1	accumulation					230:241	triacylglycerol accumulation	214:241	triacylglycerol accumulation	214:241	AIL7 over-expression modulates fatty acid biosynthesis and triacylglycerol accumulation in Arabidopsis developing seeds through the transcriptional regulation of associated genes.
34215912	0	14	from	biosynthesis	69:80	arg1	thaliana					145:152	Arabidopsis thaliana	133:152	Arabidopsis thaliana	133:152	The effect of AINTEGUMENTA-LIKE 7 over-expression on seed fatty acid biosynthesis, storage oil accumulation and the transcriptome in Arabidopsis thaliana.
34215912	6	15	theme	morphological	1221:1233	arg1	deviations					1235:1244	morphological deviations	1221:1244	morphological deviations	1221:1244	Seeds and seedlings from transgenic lines also exhibited morphological deviations compared to wild type.
34215912	1	16	from	accumulation	230:241	arg1	seeds					269:273	Arabidopsis developing seeds	246:273	Arabidopsis developing seeds	246:273	AIL7 over-expression modulates fatty acid biosynthesis and triacylglycerol accumulation in Arabidopsis developing seeds through the transcriptional regulation of associated genes.
34215912	4	17	theme	meristematic	867:878	arg1	function					880:887	meristematic function	867:887	meristematic function	867:887	Although AINTEGUMENTA-LIKE 7 (AIL7) is involved in meristematic function and shoot phyllotaxy, its effect in the context of lipid biosynthesis has yet to be assessed.
34215912	3	18	theme	INSENSITIVE	755:765	arg1	ACID					750:753	ABSCISSIC ACID INSENSITIVE 3	740:767	ABSCISSIC ACID INSENSITIVE 3	740:767	As a result, an enormous amount of attention has been dedicated towards uncovering the regulatory cascade responsible for the fine-tuning of the lipid biosynthetic pathway in seeds, which is regulated in part through the action of LEAFY COTYLEDON1, ABSCISSIC ACID INSENSITIVE 3, FUSCA3 and LEC2 (LAFL) transcription factors.
34215912	9	19	theme	lipid	1758:1762	arg1	pathway					1777:1783	the lipid biosynthetic pathway	1754:1783	the lipid biosynthetic pathway in seeds	1754:1792	These findings not only advance our understanding of the lipid biosynthetic pathway in seeds, but also provide evidence for additional functions of AIL7, which could prove valuable in downstream breeding and/or metabolic engineering endeavors.
34215912	0	20	from	accumulation	95:106	arg1	thaliana					145:152	Arabidopsis thaliana	133:152	Arabidopsis thaliana	133:152	The effect of AINTEGUMENTA-LIKE 7 over-expression on seed fatty acid biosynthesis, storage oil accumulation and the transcriptome in Arabidopsis thaliana.
34215912	6	21	theme	transgenic	1189:1198	arg1	lines					1200:1204	transgenic lines	1189:1204	transgenic lines	1189:1204	Seeds and seedlings from transgenic lines also exhibited morphological deviations compared to wild type.
34215912	6	22	from	lines	1200:1204	arg1	seedlings					1174:1182	seedlings	1174:1182	seedlings	1174:1182	Seeds and seedlings from transgenic lines also exhibited morphological deviations compared to wild type.
34215912	6	22	from	lines	1200:1204	arg1	Seeds					1164:1168	Seeds	1164:1168	Seeds	1164:1168	Seeds and seedlings from transgenic lines also exhibited morphological deviations compared to wild type.
34215912	5	23	theme	seed-specific	1007:1019	arg1	lines					1037:1041	AIL7 seed-specific over-expression lines	1002:1041	AIL7 seed-specific over-expression lines	1002:1041	Here, we generated AIL7 seed-specific over-expression lines and found that they exhibited significant alterations in FA composition and decreased total lipid accumulation in seeds.
34215912	4	24	theme	lipid	940:944	arg1	biosynthesis					946:957	lipid biosynthesis	940:957	lipid biosynthesis	940:957	Although AINTEGUMENTA-LIKE 7 (AIL7) is involved in meristematic function and shoot phyllotaxy, its effect in the context of lipid biosynthesis has yet to be assessed.
34215912	7	25	theme	genes	1344:1348	arg1	expression					1325:1334	the expression	1321:1334	the expression of many genes related to FA biosynthesis and TAG breakdown	1321:1393	Correspondingly, RNA-Seq analysis demonstrated that the expression of many genes related to FA biosynthesis and TAG breakdown were significantly altered in developing siliques from transgenic lines compared to wild-type plants.
34215912	0	26	theme	fatty	58:62	arg1	biosynthesis					69:80	seed fatty acid biosynthesis	53:80	seed fatty acid biosynthesis	53:80	The effect of AINTEGUMENTA-LIKE 7 over-expression on seed fatty acid biosynthesis, storage oil accumulation and the transcriptome in Arabidopsis thaliana.
34215912	3	27	theme	factors	807:813	arg1	action					712:717	the action	708:717	the action of LEAFY COTYLEDON1, ABSCISSIC ACID INSENSITIVE 3, FUSCA3 and LEC2 (LAFL) transcription factors	708:813	As a result, an enormous amount of attention has been dedicated towards uncovering the regulatory cascade responsible for the fine-tuning of the lipid biosynthetic pathway in seeds, which is regulated in part through the action of LEAFY COTYLEDON1, ABSCISSIC ACID INSENSITIVE 3, FUSCA3 and LEC2 (LAFL) transcription factors.
34215912	9	28	from	understanding	1737:1749	arg1	seeds					1788:1792	seeds	1788:1792	seeds	1788:1792	These findings not only advance our understanding of the lipid biosynthetic pathway in seeds, but also provide evidence for additional functions of AIL7, which could prove valuable in downstream breeding and/or metabolic engineering endeavors.
34215912	8	29	theme	AIL7	1534:1537	arg1	over-expression					1515:1529	The seed-specific over-expression	1497:1529	The seed-specific over-expression of AIL7	1497:1537	The seed-specific over-expression of AIL7 also altered the expression profiles of many genes related to starch metabolism, photosynthesis and stress response, suggesting further roles for AIL7 in plants.
34215912	0	30	theme	storage	83:89	arg1	accumulation					95:106	storage oil accumulation	83:106	storage oil accumulation	83:106	The effect of AINTEGUMENTA-LIKE 7 over-expression on seed fatty acid biosynthesis, storage oil accumulation and the transcriptome in Arabidopsis thaliana.
34215912	3	31	from	fine-tuning	617:627	arg1	seeds					666:670	seeds	666:670	seeds	666:670	As a result, an enormous amount of attention has been dedicated towards uncovering the regulatory cascade responsible for the fine-tuning of the lipid biosynthetic pathway in seeds, which is regulated in part through the action of LEAFY COTYLEDON1, ABSCISSIC ACID INSENSITIVE 3, FUSCA3 and LEC2 (LAFL) transcription factors.
34215912	5	32	theme	significant	1073:1083	arg1	alterations					1085:1095	significant alterations	1073:1095	significant alterations in FA composition	1073:1113	Here, we generated AIL7 seed-specific over-expression lines and found that they exhibited significant alterations in FA composition and decreased total lipid accumulation in seeds.
34215912	7	33	theme	many	1339:1342	arg1	genes					1344:1348	many genes	1339:1348	many genes related to FA biosynthesis and TAG breakdown	1339:1393	Correspondingly, RNA-Seq analysis demonstrated that the expression of many genes related to FA biosynthesis and TAG breakdown were significantly altered in developing siliques from transgenic lines compared to wild-type plants.
34215912	8	34	theme	expression	1556:1565	arg1	profiles					1567:1574	the expression profiles	1552:1574	the expression profiles of many genes related to starch metabolism, photosynthesis and stress response	1552:1653	The seed-specific over-expression of AIL7 also altered the expression profiles of many genes related to starch metabolism, photosynthesis and stress response, suggesting further roles for AIL7 in plants.
34215912	9	35	theme	pathway	1777:1783	arg1	understanding					1737:1749	our understanding	1733:1749	our understanding of the lipid biosynthetic pathway in seeds	1733:1792	These findings not only advance our understanding of the lipid biosynthetic pathway in seeds, but also provide evidence for additional functions of AIL7, which could prove valuable in downstream breeding and/or metabolic engineering endeavors.
34215912	5	36	from	alterations	1085:1095	arg1	composition					1103:1113	FA composition	1100:1113	FA composition	1100:1113	Here, we generated AIL7 seed-specific over-expression lines and found that they exhibited significant alterations in FA composition and decreased total lipid accumulation in seeds.
34215912	1	37	theme	associated	317:326	arg1	genes					328:332	associated genes	317:332	associated genes	317:332	AIL7 over-expression modulates fatty acid biosynthesis and triacylglycerol accumulation in Arabidopsis developing seeds through the transcriptional regulation of associated genes.
34215912	2	38	theme	broad	445:449	arg1	food					451:454	broad food	445:454	broad food	445:454	Seed fatty acids (FAs) and triacylglycerol (TAG) contribute to many functions in plants, and seed lipids have broad food, feed and industrial applications.
34215912	9	39	theme	AIL7	1849:1852	arg1	functions					1836:1844	additional functions	1825:1844	additional functions of AIL7, which could prove valuable in downstream breeding and/or metabolic engineering endeavors	1825:1942	These findings not only advance our understanding of the lipid biosynthetic pathway in seeds, but also provide evidence for additional functions of AIL7, which could prove valuable in downstream breeding and/or metabolic engineering endeavors.
34215912	8	40	theme	genes	1584:1588	arg1	profiles					1567:1574	the expression profiles	1552:1574	the expression profiles of many genes related to starch metabolism, photosynthesis and stress response	1552:1653	The seed-specific over-expression of AIL7 also altered the expression profiles of many genes related to starch metabolism, photosynthesis and stress response, suggesting further roles for AIL7 in plants.
34215912	2	41	from	functions	403:411	arg1	plants					416:421	plants	416:421	plants	416:421	Seed fatty acids (FAs) and triacylglycerol (TAG) contribute to many functions in plants, and seed lipids have broad food, feed and industrial applications.
34215912	3	42	theme	biosynthetic	642:653	arg1	pathway					655:661	the lipid biosynthetic pathway	632:661	the lipid biosynthetic pathway	632:661	As a result, an enormous amount of attention has been dedicated towards uncovering the regulatory cascade responsible for the fine-tuning of the lipid biosynthetic pathway in seeds, which is regulated in part through the action of LEAFY COTYLEDON1, ABSCISSIC ACID INSENSITIVE 3, FUSCA3 and LEC2 (LAFL) transcription factors.
34215912	5	43	theme	lipid	1135:1139	arg1	accumulation					1141:1152	total lipid accumulation	1129:1152	total lipid accumulation in seeds	1129:1161	Here, we generated AIL7 seed-specific over-expression lines and found that they exhibited significant alterations in FA composition and decreased total lipid accumulation in seeds.
34215912	9	44	from	seeds	1788:1792	arg1	understanding					1737:1749	our understanding	1733:1749	our understanding of the lipid biosynthetic pathway in seeds	1733:1792	These findings not only advance our understanding of the lipid biosynthetic pathway in seeds, but also provide evidence for additional functions of AIL7, which could prove valuable in downstream breeding and/or metabolic engineering endeavors.
34215912	1	45	theme	Arabidopsis	246:256	arg1	seeds					269:273	Arabidopsis developing seeds	246:273	Arabidopsis developing seeds	246:273	AIL7 over-expression modulates fatty acid biosynthesis and triacylglycerol accumulation in Arabidopsis developing seeds through the transcriptional regulation of associated genes.
34215912	9	46	from	pathway	1777:1783	arg1	seeds					1788:1792	seeds	1788:1792	seeds	1788:1792	These findings not only advance our understanding of the lipid biosynthetic pathway in seeds, but also provide evidence for additional functions of AIL7, which could prove valuable in downstream breeding and/or metabolic engineering endeavors.
34215912	3	47	theme	enormous	507:514	arg1	result					496:501	a result	494:501	a result	494:501	As a result, an enormous amount of attention has been dedicated towards uncovering the regulatory cascade responsible for the fine-tuning of the lipid biosynthetic pathway in seeds, which is regulated in part through the action of LEAFY COTYLEDON1, ABSCISSIC ACID INSENSITIVE 3, FUSCA3 and LEC2 (LAFL) transcription factors.
34215912	3	47	theme	enormous	507:514	arg1	attention					526:534	attention	526:534	attention	526:534	As a result, an enormous amount of attention has been dedicated towards uncovering the regulatory cascade responsible for the fine-tuning of the lipid biosynthetic pathway in seeds, which is regulated in part through the action of LEAFY COTYLEDON1, ABSCISSIC ACID INSENSITIVE 3, FUSCA3 and LEC2 (LAFL) transcription factors.
34215912	3	47	theme	enormous	507:514	arg1	amount					516:521	an enormous amount	504:521	an enormous amount of attention	504:534	As a result, an enormous amount of attention has been dedicated towards uncovering the regulatory cascade responsible for the fine-tuning of the lipid biosynthetic pathway in seeds, which is regulated in part through the action of LEAFY COTYLEDON1, ABSCISSIC ACID INSENSITIVE 3, FUSCA3 and LEC2 (LAFL) transcription factors.
34215912	8	48	theme	stress	1639:1644	arg1	response					1646:1653	stress response	1639:1653	stress response	1639:1653	The seed-specific over-expression of AIL7 also altered the expression profiles of many genes related to starch metabolism, photosynthesis and stress response, suggesting further roles for AIL7 in plants.
34215912	9	49	theme	metabolic	1912:1920	arg1	endeavors					1934:1942	metabolic engineering endeavors	1912:1942	metabolic engineering endeavors	1912:1942	These findings not only advance our understanding of the lipid biosynthetic pathway in seeds, but also provide evidence for additional functions of AIL7, which could prove valuable in downstream breeding and/or metabolic engineering endeavors.
34215912	2	50	theme	Seed	335:338	arg1	acids					346:350	Seed fatty acids	335:350	Seed fatty acids (FAs)	335:356	Seed fatty acids (FAs) and triacylglycerol (TAG) contribute to many functions in plants, and seed lipids have broad food, feed and industrial applications.
34215912	2	50	theme	Seed	335:338	arg1	FAs					353:355	FAs	353:355	FAs	353:355	Seed fatty acids (FAs) and triacylglycerol (TAG) contribute to many functions in plants, and seed lipids have broad food, feed and industrial applications.
34215912	7	51	theme	transgenic	1450:1459	arg1	lines					1461:1465	transgenic lines	1450:1465	transgenic lines	1450:1465	Correspondingly, RNA-Seq analysis demonstrated that the expression of many genes related to FA biosynthesis and TAG breakdown were significantly altered in developing siliques from transgenic lines compared to wild-type plants.
34215912	8	52	theme	further	1667:1673	arg1	roles					1675:1679	further roles	1667:1679	further roles for AIL7 in plants	1667:1698	The seed-specific over-expression of AIL7 also altered the expression profiles of many genes related to starch metabolism, photosynthesis and stress response, suggesting further roles for AIL7 in plants.
34215912	3	53	theme	LEAFY	722:726	arg1	COTYLEDON1					728:737	LEAFY COTYLEDON1	722:737	LEAFY COTYLEDON1	722:737	As a result, an enormous amount of attention has been dedicated towards uncovering the regulatory cascade responsible for the fine-tuning of the lipid biosynthetic pathway in seeds, which is regulated in part through the action of LEAFY COTYLEDON1, ABSCISSIC ACID INSENSITIVE 3, FUSCA3 and LEC2 (LAFL) transcription factors.
34215912	6	54	theme	wild	1258:1261	arg1	type					1263:1266	wild type	1258:1266	wild type	1258:1266	Seeds and seedlings from transgenic lines also exhibited morphological deviations compared to wild type.
34215912	1	55	theme	acid	192:195	arg1	biosynthesis					197:208	fatty acid biosynthesis	186:208	fatty acid biosynthesis	186:208	AIL7 over-expression modulates fatty acid biosynthesis and triacylglycerol accumulation in Arabidopsis developing seeds through the transcriptional regulation of associated genes.
34215912	3	56	theme	ACID	750:753	arg1	action					712:717	the action	708:717	the action of LEAFY COTYLEDON1, ABSCISSIC ACID INSENSITIVE 3, FUSCA3 and LEC2 (LAFL) transcription factors	708:813	As a result, an enormous amount of attention has been dedicated towards uncovering the regulatory cascade responsible for the fine-tuning of the lipid biosynthetic pathway in seeds, which is regulated in part through the action of LEAFY COTYLEDON1, ABSCISSIC ACID INSENSITIVE 3, FUSCA3 and LEC2 (LAFL) transcription factors.
34215912	4	57	theme	shoot	893:897	arg1	phyllotaxy					899:908	shoot phyllotaxy	893:908	shoot phyllotaxy	893:908	Although AINTEGUMENTA-LIKE 7 (AIL7) is involved in meristematic function and shoot phyllotaxy, its effect in the context of lipid biosynthesis has yet to be assessed.
34215912	0	58	theme	Arabidopsis	133:143	arg1	thaliana					145:152	Arabidopsis thaliana	133:152	Arabidopsis thaliana	133:152	The effect of AINTEGUMENTA-LIKE 7 over-expression on seed fatty acid biosynthesis, storage oil accumulation and the transcriptome in Arabidopsis thaliana.
34215912	3	59	theme	responsible	597:607	arg1	cascade					589:595	the regulatory cascade	574:595	the regulatory cascade responsible for the fine-tuning of the lipid biosynthetic pathway in seeds, which is regulated in part through the action of LEAFY COTYLEDON1, ABSCISSIC ACID INSENSITIVE 3, FUSCA3 and LEC2 (LAFL) transcription factors	574:813	As a result, an enormous amount of attention has been dedicated towards uncovering the regulatory cascade responsible for the fine-tuning of the lipid biosynthetic pathway in seeds, which is regulated in part through the action of LEAFY COTYLEDON1, ABSCISSIC ACID INSENSITIVE 3, FUSCA3 and LEC2 (LAFL) transcription factors.
34215912	3	60	theme	FUSCA3	770:775	arg1	action					712:717	the action	708:717	the action of LEAFY COTYLEDON1, ABSCISSIC ACID INSENSITIVE 3, FUSCA3 and LEC2 (LAFL) transcription factors	708:813	As a result, an enormous amount of attention has been dedicated towards uncovering the regulatory cascade responsible for the fine-tuning of the lipid biosynthetic pathway in seeds, which is regulated in part through the action of LEAFY COTYLEDON1, ABSCISSIC ACID INSENSITIVE 3, FUSCA3 and LEC2 (LAFL) transcription factors.
34215912	8	61	from	roles	1675:1679	arg1	plants					1693:1698	plants	1693:1698	plants	1693:1698	The seed-specific over-expression of AIL7 also altered the expression profiles of many genes related to starch metabolism, photosynthesis and stress response, suggesting further roles for AIL7 in plants.
34215912	3	62	theme	regulatory	578:587	arg1	cascade					589:595	the regulatory cascade	574:595	the regulatory cascade responsible for the fine-tuning of the lipid biosynthetic pathway in seeds, which is regulated in part through the action of LEAFY COTYLEDON1, ABSCISSIC ACID INSENSITIVE 3, FUSCA3 and LEC2 (LAFL) transcription factors	574:813	As a result, an enormous amount of attention has been dedicated towards uncovering the regulatory cascade responsible for the fine-tuning of the lipid biosynthetic pathway in seeds, which is regulated in part through the action of LEAFY COTYLEDON1, ABSCISSIC ACID INSENSITIVE 3, FUSCA3 and LEC2 (LAFL) transcription factors.
34215912	5	63	theme	AIL7	1002:1005	arg1	lines					1037:1041	AIL7 seed-specific over-expression lines	1002:1041	AIL7 seed-specific over-expression lines	1002:1041	Here, we generated AIL7 seed-specific over-expression lines and found that they exhibited significant alterations in FA composition and decreased total lipid accumulation in seeds.
34215912	9	64	theme	biosynthetic	1764:1775	arg1	pathway					1777:1783	the lipid biosynthetic pathway	1754:1783	the lipid biosynthetic pathway in seeds	1754:1792	These findings not only advance our understanding of the lipid biosynthetic pathway in seeds, but also provide evidence for additional functions of AIL7, which could prove valuable in downstream breeding and/or metabolic engineering endeavors.
34215912	8	65	theme	seed-specific	1501:1513	arg1	over-expression					1515:1529	The seed-specific over-expression	1497:1529	The seed-specific over-expression of AIL7	1497:1537	The seed-specific over-expression of AIL7 also altered the expression profiles of many genes related to starch metabolism, photosynthesis and stress response, suggesting further roles for AIL7 in plants.
34215912	7	66	theme	FA	1361:1362	arg1	biosynthesis					1364:1375	FA biosynthesis	1361:1375	FA biosynthesis	1361:1375	Correspondingly, RNA-Seq analysis demonstrated that the expression of many genes related to FA biosynthesis and TAG breakdown were significantly altered in developing siliques from transgenic lines compared to wild-type plants.
34215912	3	67	theme	LAFL	787:790	arg1	factors					807:813	LEC2 (LAFL) transcription factors	781:813	LEC2 (LAFL) transcription factors	781:813	As a result, an enormous amount of attention has been dedicated towards uncovering the regulatory cascade responsible for the fine-tuning of the lipid biosynthetic pathway in seeds, which is regulated in part through the action of LEAFY COTYLEDON1, ABSCISSIC ACID INSENSITIVE 3, FUSCA3 and LEC2 (LAFL) transcription factors.
34215912	0	68	theme	acid	64:67	arg1	biosynthesis					69:80	seed fatty acid biosynthesis	53:80	seed fatty acid biosynthesis	53:80	The effect of AINTEGUMENTA-LIKE 7 over-expression on seed fatty acid biosynthesis, storage oil accumulation and the transcriptome in Arabidopsis thaliana.
34215912	5	69	theme	over-expression	1021:1035	arg1	lines					1037:1041	AIL7 seed-specific over-expression lines	1002:1041	AIL7 seed-specific over-expression lines	1002:1041	Here, we generated AIL7 seed-specific over-expression lines and found that they exhibited significant alterations in FA composition and decreased total lipid accumulation in seeds.
34215912	1	70	from	biosynthesis	197:208	arg1	seeds					269:273	Arabidopsis developing seeds	246:273	Arabidopsis developing seeds	246:273	AIL7 over-expression modulates fatty acid biosynthesis and triacylglycerol accumulation in Arabidopsis developing seeds through the transcriptional regulation of associated genes.
34215912	7	71	theme	related	1350:1356	arg1	genes					1344:1348	many genes	1339:1348	many genes related to FA biosynthesis and TAG breakdown	1339:1393	Correspondingly, RNA-Seq analysis demonstrated that the expression of many genes related to FA biosynthesis and TAG breakdown were significantly altered in developing siliques from transgenic lines compared to wild-type plants.
34215912	3	72	theme	LEC2	781:784	arg1	factors					807:813	LEC2 (LAFL) transcription factors	781:813	LEC2 (LAFL) transcription factors	781:813	As a result, an enormous amount of attention has been dedicated towards uncovering the regulatory cascade responsible for the fine-tuning of the lipid biosynthetic pathway in seeds, which is regulated in part through the action of LEAFY COTYLEDON1, ABSCISSIC ACID INSENSITIVE 3, FUSCA3 and LEC2 (LAFL) transcription factors.
34215912	5	73	theme	FA	1100:1101	arg1	composition					1103:1113	FA composition	1100:1113	FA composition	1100:1113	Here, we generated AIL7 seed-specific over-expression lines and found that they exhibited significant alterations in FA composition and decreased total lipid accumulation in seeds.
34215912	1	74	theme	AIL7	155:158	arg1	over-expression					160:174	AIL7 over-expression	155:174	AIL7 over-expression	155:174	AIL7 over-expression modulates fatty acid biosynthesis and triacylglycerol accumulation in Arabidopsis developing seeds through the transcriptional regulation of associated genes.
34215912	4	75	theme	biosynthesis	946:957	arg1	context					929:935	the context	925:935	the context of lipid biosynthesis	925:957	Although AINTEGUMENTA-LIKE 7 (AIL7) is involved in meristematic function and shoot phyllotaxy, its effect in the context of lipid biosynthesis has yet to be assessed.
34215912	0	76	from	effect	4:9	arg1	biosynthesis					69:80	seed fatty acid biosynthesis	53:80	seed fatty acid biosynthesis	53:80	The effect of AINTEGUMENTA-LIKE 7 over-expression on seed fatty acid biosynthesis, storage oil accumulation and the transcriptome in Arabidopsis thaliana.
34215912	0	76	from	effect	4:9	arg1	accumulation					95:106	storage oil accumulation	83:106	storage oil accumulation	83:106	The effect of AINTEGUMENTA-LIKE 7 over-expression on seed fatty acid biosynthesis, storage oil accumulation and the transcriptome in Arabidopsis thaliana.
34215912	0	76	from	effect	4:9	arg1	transcriptome					116:128	the transcriptome	112:128	the transcriptome	112:128	The effect of AINTEGUMENTA-LIKE 7 over-expression on seed fatty acid biosynthesis, storage oil accumulation and the transcriptome in Arabidopsis thaliana.
34215912	0	77	theme	oil	91:93	arg1	accumulation					95:106	storage oil accumulation	83:106	storage oil accumulation	83:106	The effect of AINTEGUMENTA-LIKE 7 over-expression on seed fatty acid biosynthesis, storage oil accumulation and the transcriptome in Arabidopsis thaliana.
34215912	3	78	theme	transcription	793:805	arg1	factors					807:813	LEC2 (LAFL) transcription factors	781:813	LEC2 (LAFL) transcription factors	781:813	As a result, an enormous amount of attention has been dedicated towards uncovering the regulatory cascade responsible for the fine-tuning of the lipid biosynthetic pathway in seeds, which is regulated in part through the action of LEAFY COTYLEDON1, ABSCISSIC ACID INSENSITIVE 3, FUSCA3 and LEC2 (LAFL) transcription factors.
34215912	9	79	theme	additional	1825:1834	arg1	functions					1836:1844	additional functions	1825:1844	additional functions of AIL7, which could prove valuable in downstream breeding and/or metabolic engineering endeavors	1825:1942	These findings not only advance our understanding of the lipid biosynthetic pathway in seeds, but also provide evidence for additional functions of AIL7, which could prove valuable in downstream breeding and/or metabolic engineering endeavors.
34215912	8	80	theme	many	1579:1582	arg1	genes					1584:1588	many genes	1579:1588	many genes related to starch metabolism, photosynthesis and stress response	1579:1653	The seed-specific over-expression of AIL7 also altered the expression profiles of many genes related to starch metabolism, photosynthesis and stress response, suggesting further roles for AIL7 in plants.
34215912	2	81	theme	many	398:401	arg1	functions					403:411	many functions	398:411	many functions in plants	398:421	Seed fatty acids (FAs) and triacylglycerol (TAG) contribute to many functions in plants, and seed lipids have broad food, feed and industrial applications.
34215912	0	82	theme	AINTEGUMENTA-LIKE	14:30	arg1	over-expression					34:48	AINTEGUMENTA-LIKE 7 over-expression	14:48	AINTEGUMENTA-LIKE 7 over-expression	14:48	The effect of AINTEGUMENTA-LIKE 7 over-expression on seed fatty acid biosynthesis, storage oil accumulation and the transcriptome in Arabidopsis thaliana.
34215912	1	83	theme	genes	328:332	arg1	regulation					303:312	the transcriptional regulation	283:312	the transcriptional regulation of associated genes	283:332	AIL7 over-expression modulates fatty acid biosynthesis and triacylglycerol accumulation in Arabidopsis developing seeds through the transcriptional regulation of associated genes.
34215912	7	84	theme	TAG	1381:1383	arg1	breakdown					1385:1393	TAG breakdown	1381:1393	TAG breakdown	1381:1393	Correspondingly, RNA-Seq analysis demonstrated that the expression of many genes related to FA biosynthesis and TAG breakdown were significantly altered in developing siliques from transgenic lines compared to wild-type plants.
34215912	8	85	theme	related	1590:1596	arg1	genes					1584:1588	many genes	1579:1588	many genes related to starch metabolism, photosynthesis and stress response	1579:1653	The seed-specific over-expression of AIL7 also altered the expression profiles of many genes related to starch metabolism, photosynthesis and stress response, suggesting further roles for AIL7 in plants.
34215912	3	86	theme	pathway	655:661	arg1	fine-tuning					617:627	the fine-tuning	613:627	the fine-tuning of the lipid biosynthetic pathway in seeds, which is regulated in part through the action of LEAFY COTYLEDON1, ABSCISSIC ACID INSENSITIVE 3, FUSCA3 and LEC2 (LAFL) transcription factors	613:813	As a result, an enormous amount of attention has been dedicated towards uncovering the regulatory cascade responsible for the fine-tuning of the lipid biosynthetic pathway in seeds, which is regulated in part through the action of LEAFY COTYLEDON1, ABSCISSIC ACID INSENSITIVE 3, FUSCA3 and LEC2 (LAFL) transcription factors.
34215912	2	87	theme	seed	428:431	arg1	lipids					433:438	seed lipids	428:438	seed lipids	428:438	Seed fatty acids (FAs) and triacylglycerol (TAG) contribute to many functions in plants, and seed lipids have broad food, feed and industrial applications.
34215912	0	88	theme	over-expression	34:48	arg1	effect					4:9	The effect	0:9	The effect of AINTEGUMENTA-LIKE 7 over-expression on seed fatty acid biosynthesis, storage oil accumulation and the transcriptome in Arabidopsis thaliana.	0:153	The effect of AINTEGUMENTA-LIKE 7 over-expression on seed fatty acid biosynthesis, storage oil accumulation and the transcriptome in Arabidopsis thaliana.
34215912	7	89	theme	RNA-Seq	1286:1292	arg1	analysis					1294:1301	RNA-Seq analysis	1286:1301	RNA-Seq analysis	1286:1301	Correspondingly, RNA-Seq analysis demonstrated that the expression of many genes related to FA biosynthesis and TAG breakdown were significantly altered in developing siliques from transgenic lines compared to wild-type plants.
34215912	2	90	theme	industrial	466:475	arg1	applications					477:488	industrial applications	466:488	industrial applications	466:488	Seed fatty acids (FAs) and triacylglycerol (TAG) contribute to many functions in plants, and seed lipids have broad food, feed and industrial applications.
34215912	8	91	theme	starch	1601:1606	arg1	metabolism					1608:1617	starch metabolism	1601:1617	starch metabolism	1601:1617	The seed-specific over-expression of AIL7 also altered the expression profiles of many genes related to starch metabolism, photosynthesis and stress response, suggesting further roles for AIL7 in plants.
34215912	3	92	theme	lipid	636:640	arg1	pathway					655:661	the lipid biosynthetic pathway	632:661	the lipid biosynthetic pathway	632:661	As a result, an enormous amount of attention has been dedicated towards uncovering the regulatory cascade responsible for the fine-tuning of the lipid biosynthetic pathway in seeds, which is regulated in part through the action of LEAFY COTYLEDON1, ABSCISSIC ACID INSENSITIVE 3, FUSCA3 and LEC2 (LAFL) transcription factors.
34215912	0	93	theme	seed	53:56	arg1	biosynthesis					69:80	seed fatty acid biosynthesis	53:80	seed fatty acid biosynthesis	53:80	The effect of AINTEGUMENTA-LIKE 7 over-expression on seed fatty acid biosynthesis, storage oil accumulation and the transcriptome in Arabidopsis thaliana.
34215912	5	94	theme	total	1129:1133	arg1	accumulation					1141:1152	total lipid accumulation	1129:1152	total lipid accumulation in seeds	1129:1161	Here, we generated AIL7 seed-specific over-expression lines and found that they exhibited significant alterations in FA composition and decreased total lipid accumulation in seeds.
34215912	1	95	theme	developing	258:267	arg1	seeds					269:273	Arabidopsis developing seeds	246:273	Arabidopsis developing seeds	246:273	AIL7 over-expression modulates fatty acid biosynthesis and triacylglycerol accumulation in Arabidopsis developing seeds through the transcriptional regulation of associated genes.
34215912	9	96	theme	downstream	1885:1894	arg1	breeding					1896:1903	downstream breeding	1885:1903	downstream breeding	1885:1903	These findings not only advance our understanding of the lipid biosynthetic pathway in seeds, but also provide evidence for additional functions of AIL7, which could prove valuable in downstream breeding and/or metabolic engineering endeavors.
34215912	0	97	from	transcriptome	116:128	arg1	thaliana					145:152	Arabidopsis thaliana	133:152	Arabidopsis thaliana	133:152	The effect of AINTEGUMENTA-LIKE 7 over-expression on seed fatty acid biosynthesis, storage oil accumulation and the transcriptome in Arabidopsis thaliana.
32845255	10	0	theme	antibiotic	1336:1345	arg1	treatment					1347:1355	antibiotic treatment	1336:1355	antibiotic treatment	1336:1355	In conclusion, quercetin is effective in recovering gut microbiota in mice after antibiotic treatment and may act as a prebiotic in combatting gut dysbiosis.
32845255	5	1	theme	high-throughput	522:536	arg1	sequencing					538:547	16S rDNA high-throughput sequencing	513:547	16S rDNA high-throughput sequencing	513:547	Gas chromatography and 16S rDNA high-throughput sequencing techniques were used to investigate short-chain fatty acid content and gut microbial diversity and composition.
32845255	1	2	from	activity	79:86	arg1	humans					122:127	humans	122:127	humans	122:127	The diversity and activity of the gut microbiota residing in humans and animals are significantly influenced by the diet.
32845255	1	2	from	activity	79:86	arg1	animals					133:139	animals	133:139	animals	133:139	The diversity and activity of the gut microbiota residing in humans and animals are significantly influenced by the diet.
32845255	5	3	theme	acid	603:606	arg1	content					608:614	short-chain fatty acid content	585:614	short-chain fatty acid content	585:614	Gas chromatography and 16S rDNA high-throughput sequencing techniques were used to investigate short-chain fatty acid content and gut microbial diversity and composition.
32845255	4	4	with	treatment	451:459	arg1	cocktail					480:487	an antibiotic cocktail	466:487	an antibiotic cocktail	466:487	Gut dysbiosis was successfully induced in mice by treatment with an antibiotic cocktail.
32845255	8	5	theme	villi	1031:1035	arg1	length					1010:1015	The length	1006:1015	The length of intestinal villi and mucosal thickness	1006:1057	The length of intestinal villi and mucosal thickness were also significantly increased in response to quercetin treatment (P < 0.05).
32845255	5	6	theme	chromatography	494:507	arg1	techniques					549:558	Gas chromatography and 16S rDNA high-throughput sequencing techniques	490:558	Gas chromatography and 16S rDNA high-throughput sequencing techniques	490:558	Gas chromatography and 16S rDNA high-throughput sequencing techniques were used to investigate short-chain fatty acid content and gut microbial diversity and composition.
32845255	1	7	theme	microbiota	99:108	arg1	residing					110:117	the gut microbiota residing	91:117	the gut microbiota residing	91:117	The diversity and activity of the gut microbiota residing in humans and animals are significantly influenced by the diet.
32845255	7	8	theme	P	995:995	arg1	oxidase					986:992	serum diamine oxidase	972:992	serum diamine oxidase (P < 0.05)	972:1003	Meanwhile, intestinal barrier function was also recovered remarkably as indicated by a decrease in the content of serum d-lactic acid and the activity of serum diamine oxidase (P < 0.05).
32845255	7	8	theme	P	995:995	arg1	<					997:997	P < 0.05	995:1002	P < 0.05	995:1002	Meanwhile, intestinal barrier function was also recovered remarkably as indicated by a decrease in the content of serum d-lactic acid and the activity of serum diamine oxidase (P < 0.05).
32845255	8	9	theme	intestinal	1020:1029	arg1	villi					1031:1035	intestinal villi	1020:1035	intestinal villi	1020:1035	The length of intestinal villi and mucosal thickness were also significantly increased in response to quercetin treatment (P < 0.05).
32845255	7	10	theme	barrier	840:846	arg1	function					848:855	intestinal barrier function	829:855	intestinal barrier function	829:855	Meanwhile, intestinal barrier function was also recovered remarkably as indicated by a decrease in the content of serum d-lactic acid and the activity of serum diamine oxidase (P < 0.05).
32845255	2	11	contain	possesses	248:256	arg1	polyphenols					220:230	the representative polyphenols	201:230	the representative polyphenols in human diets	201:245	Quercetin, one of the representative polyphenols in human diets, possesses a wide range of biological properties.
32845255	2	11	contain	possesses	248:256	arg1	one					194:196	one	194:196	one	194:196	Quercetin, one of the representative polyphenols in human diets, possesses a wide range of biological properties.
32845255	2	11	contain	possesses	248:256	arg1	Quercetin					183:191	Quercetin	183:191	Quercetin	183:191	Quercetin, one of the representative polyphenols in human diets, possesses a wide range of biological properties.
32845255	2	11	contain	possesses	248:256	arg2	range					265:269	a wide range	258:269	a wide range of biological properties	258:294	Quercetin, one of the representative polyphenols in human diets, possesses a wide range of biological properties.
32845255	9	12	from	production	1157:1166	arg1	faeces					1183:1188	faeces	1183:1188	faeces	1183:1188	Furthermore, the production of butyrate in faeces was enhanced significantly in quercetin-treated mice (P < 0.05).
32845255	3	13	from	effects	352:358	arg1	mice					395:398	antibiotic-treated mice	376:398	antibiotic-treated mice	376:398	The aim of this study was to investigate the prebiotic effects of quercetin in antibiotic-treated mice.
32845255	5	14	theme	microbial	624:632	arg1	diversity					634:642	gut microbial diversity	620:642	gut microbial diversity	620:642	Gas chromatography and 16S rDNA high-throughput sequencing techniques were used to investigate short-chain fatty acid content and gut microbial diversity and composition.
32845255	5	15	theme	gut	620:622	arg1	diversity					634:642	gut microbial diversity	620:642	gut microbial diversity	620:642	Gas chromatography and 16S rDNA high-throughput sequencing techniques were used to investigate short-chain fatty acid content and gut microbial diversity and composition.
32845255	2	16	theme	wide	260:263	arg1	range					265:269	a wide range	258:269	a wide range of biological properties	258:294	Quercetin, one of the representative polyphenols in human diets, possesses a wide range of biological properties.
32845255	4	17	theme	antibiotic	469:478	arg1	cocktail					480:487	an antibiotic cocktail	466:487	an antibiotic cocktail	466:487	Gut dysbiosis was successfully induced in mice by treatment with an antibiotic cocktail.
32845255	6	18	from	diversity	738:746	arg1	mice					801:804	antibiotic-treated mice	782:804	antibiotic-treated mice (P < 0.05)	782:815	The results showed that quercetin supplementation significantly improved the diversity of the gut bacterial community in antibiotic-treated mice (P < 0.05).
32845255	6	18	from	diversity	738:746	arg1	<					809:809	P < 0.05	807:814	P < 0.05	807:814	The results showed that quercetin supplementation significantly improved the diversity of the gut bacterial community in antibiotic-treated mice (P < 0.05).
32845255	5	19	theme	Gas	490:492	arg1	chromatography					494:507	Gas chromatography	490:507	Gas chromatography	490:507	Gas chromatography and 16S rDNA high-throughput sequencing techniques were used to investigate short-chain fatty acid content and gut microbial diversity and composition.
32845255	7	20	theme	acid	947:950	arg1	content					921:927	the content	917:927	the content of serum d-lactic acid	917:950	Meanwhile, intestinal barrier function was also recovered remarkably as indicated by a decrease in the content of serum d-lactic acid and the activity of serum diamine oxidase (P < 0.05).
32845255	7	21	from	decrease	905:912	arg1	content					921:927	the content	917:927	the content of serum d-lactic acid	917:950	Meanwhile, intestinal barrier function was also recovered remarkably as indicated by a decrease in the content of serum d-lactic acid and the activity of serum diamine oxidase (P < 0.05).
32845255	2	22	from	polyphenols	220:230	arg1	diets					241:245	human diets	235:245	human diets	235:245	Quercetin, one of the representative polyphenols in human diets, possesses a wide range of biological properties.
32845255	10	23	from	microbiota	1311:1320	arg1	mice					1325:1328	mice	1325:1328	mice	1325:1328	In conclusion, quercetin is effective in recovering gut microbiota in mice after antibiotic treatment and may act as a prebiotic in combatting gut dysbiosis.
32845255	3	24	theme	quercetin	363:371	arg1	effects					352:358	the prebiotic effects	338:358	the prebiotic effects of quercetin in antibiotic-treated mice	338:398	The aim of this study was to investigate the prebiotic effects of quercetin in antibiotic-treated mice.
32845255	8	25	theme	thickness	1049:1057	arg1	length					1010:1015	The length	1006:1015	The length of intestinal villi and mucosal thickness	1006:1057	The length of intestinal villi and mucosal thickness were also significantly increased in response to quercetin treatment (P < 0.05).
32845255	5	26	theme	short-chain	585:595	arg1	acid					603:606	short-chain fatty acid	585:606	short-chain fatty acid content	585:614	Gas chromatography and 16S rDNA high-throughput sequencing techniques were used to investigate short-chain fatty acid content and gut microbial diversity and composition.
32845255	8	27	theme	mucosal	1041:1047	arg1	thickness					1049:1057	mucosal thickness	1041:1057	mucosal thickness	1041:1057	The length of intestinal villi and mucosal thickness were also significantly increased in response to quercetin treatment (P < 0.05).
32845255	3	28	theme	prebiotic	342:350	arg1	effects					352:358	the prebiotic effects	338:358	the prebiotic effects of quercetin in antibiotic-treated mice	338:398	The aim of this study was to investigate the prebiotic effects of quercetin in antibiotic-treated mice.
32845255	7	29	theme	d-lactic	938:945	arg1	acid					947:950	serum d-lactic acid	932:950	serum d-lactic acid	932:950	Meanwhile, intestinal barrier function was also recovered remarkably as indicated by a decrease in the content of serum d-lactic acid and the activity of serum diamine oxidase (P < 0.05).
32845255	5	30	theme	fatty	597:601	arg1	acid					603:606	short-chain fatty acid	585:606	short-chain fatty acid content	585:614	Gas chromatography and 16S rDNA high-throughput sequencing techniques were used to investigate short-chain fatty acid content and gut microbial diversity and composition.
32845255	2	31	theme	properties	285:294	arg1	range					265:269	a wide range	258:269	a wide range of biological properties	258:294	Quercetin, one of the representative polyphenols in human diets, possesses a wide range of biological properties.
32845255	7	32	theme	serum	972:976	arg1	oxidase					986:992	serum diamine oxidase	972:992	serum diamine oxidase (P < 0.05)	972:1003	Meanwhile, intestinal barrier function was also recovered remarkably as indicated by a decrease in the content of serum d-lactic acid and the activity of serum diamine oxidase (P < 0.05).
32845255	7	32	theme	serum	972:976	arg1	<					997:997	P < 0.05	995:1002	P < 0.05	995:1002	Meanwhile, intestinal barrier function was also recovered remarkably as indicated by a decrease in the content of serum d-lactic acid and the activity of serum diamine oxidase (P < 0.05).
32845255	0	33	theme	gut	19:21	arg1	dysbiosis					23:31	gut dysbiosis	19:31	gut dysbiosis in antibiotic-treated mice	19:58	Quercetin improves gut dysbiosis in antibiotic-treated mice.
32845255	7	34	theme	oxidase	986:992	arg1	decrease					905:912	a decrease	903:912	a decrease in the content of serum d-lactic acid	903:950	Meanwhile, intestinal barrier function was also recovered remarkably as indicated by a decrease in the content of serum d-lactic acid and the activity of serum diamine oxidase (P < 0.05).
32845255	7	34	theme	oxidase	986:992	arg1	activity					960:967	the activity	956:967	the activity of serum diamine oxidase (P < 0.05)	956:1003	Meanwhile, intestinal barrier function was also recovered remarkably as indicated by a decrease in the content of serum d-lactic acid and the activity of serum diamine oxidase (P < 0.05).
32845255	2	35	theme	biological	274:283	arg1	properties					285:294	biological properties	274:294	biological properties	274:294	Quercetin, one of the representative polyphenols in human diets, possesses a wide range of biological properties.
32845255	6	36	theme	quercetin	685:693	arg1	supplementation					695:709	quercetin supplementation	685:709	quercetin supplementation	685:709	The results showed that quercetin supplementation significantly improved the diversity of the gut bacterial community in antibiotic-treated mice (P < 0.05).
32845255	0	37	theme	antibiotic-treated	36:53	arg1	mice					55:58	antibiotic-treated mice	36:58	antibiotic-treated mice	36:58	Quercetin improves gut dysbiosis in antibiotic-treated mice.
32845255	8	38	dep	increased	1083:1091	arg1	<					1131:1131	P < 0.05	1129:1136	P < 0.05	1129:1136	The length of intestinal villi and mucosal thickness were also significantly increased in response to quercetin treatment (P < 0.05).
32845255	9	39	theme	quercetin-treated	1220:1236	arg1	mice					1238:1241	quercetin-treated mice	1220:1241	quercetin-treated mice (P < 0.05)	1220:1252	Furthermore, the production of butyrate in faeces was enhanced significantly in quercetin-treated mice (P < 0.05).
32845255	9	39	theme	quercetin-treated	1220:1236	arg1	<					1246:1246	P < 0.05	1244:1251	P < 0.05	1244:1251	Furthermore, the production of butyrate in faeces was enhanced significantly in quercetin-treated mice (P < 0.05).
32845255	2	40	theme	polyphenols	220:230	arg1	polyphenols					220:230	the representative polyphenols	201:230	the representative polyphenols in human diets	201:245	Quercetin, one of the representative polyphenols in human diets, possesses a wide range of biological properties.
32845255	2	40	theme	polyphenols	220:230	arg1	one					194:196	one	194:196	one	194:196	Quercetin, one of the representative polyphenols in human diets, possesses a wide range of biological properties.
32845255	2	40	theme	polyphenols	220:230	arg1	Quercetin					183:191	Quercetin	183:191	Quercetin	183:191	Quercetin, one of the representative polyphenols in human diets, possesses a wide range of biological properties.
32845255	5	41	theme	sequencing	538:547	arg1	techniques					549:558	Gas chromatography and 16S rDNA high-throughput sequencing techniques	490:558	Gas chromatography and 16S rDNA high-throughput sequencing techniques	490:558	Gas chromatography and 16S rDNA high-throughput sequencing techniques were used to investigate short-chain fatty acid content and gut microbial diversity and composition.
32845255	6	42	theme	bacterial	759:767	arg1	community					769:777	the gut bacterial community	751:777	the gut bacterial community	751:777	The results showed that quercetin supplementation significantly improved the diversity of the gut bacterial community in antibiotic-treated mice (P < 0.05).
32845255	7	43	theme	intestinal	829:838	arg1	function					848:855	intestinal barrier function	829:855	intestinal barrier function	829:855	Meanwhile, intestinal barrier function was also recovered remarkably as indicated by a decrease in the content of serum d-lactic acid and the activity of serum diamine oxidase (P < 0.05).
32845255	2	44	theme	representative	205:218	arg1	polyphenols					220:230	the representative polyphenols	201:230	the representative polyphenols in human diets	201:245	Quercetin, one of the representative polyphenols in human diets, possesses a wide range of biological properties.
32845255	10	45	theme	gut	1307:1309	arg1	microbiota					1311:1320	gut microbiota	1307:1320	gut microbiota in mice	1307:1328	In conclusion, quercetin is effective in recovering gut microbiota in mice after antibiotic treatment and may act as a prebiotic in combatting gut dysbiosis.
32845255	1	46	theme	residing	110:117	arg1	activity					79:86	activity	79:86	activity	79:86	The diversity and activity of the gut microbiota residing in humans and animals are significantly influenced by the diet.
32845255	1	46	theme	residing	110:117	arg1	diversity					65:73	diversity	65:73	diversity	65:73	The diversity and activity of the gut microbiota residing in humans and animals are significantly influenced by the diet.
32845255	3	47	theme	antibiotic-treated	376:393	arg1	mice					395:398	antibiotic-treated mice	376:398	antibiotic-treated mice	376:398	The aim of this study was to investigate the prebiotic effects of quercetin in antibiotic-treated mice.
32845255	9	48	theme	P	1244:1244	arg1	mice					1238:1241	quercetin-treated mice	1220:1241	quercetin-treated mice (P < 0.05)	1220:1252	Furthermore, the production of butyrate in faeces was enhanced significantly in quercetin-treated mice (P < 0.05).
32845255	9	48	theme	P	1244:1244	arg1	<					1246:1246	P < 0.05	1244:1251	P < 0.05	1244:1251	Furthermore, the production of butyrate in faeces was enhanced significantly in quercetin-treated mice (P < 0.05).
32845255	6	49	theme	antibiotic-treated	782:799	arg1	mice					801:804	antibiotic-treated mice	782:804	antibiotic-treated mice (P < 0.05)	782:815	The results showed that quercetin supplementation significantly improved the diversity of the gut bacterial community in antibiotic-treated mice (P < 0.05).
32845255	6	49	theme	antibiotic-treated	782:799	arg1	<					809:809	P < 0.05	807:814	P < 0.05	807:814	The results showed that quercetin supplementation significantly improved the diversity of the gut bacterial community in antibiotic-treated mice (P < 0.05).
32845255	10	50	theme	gut	1398:1400	arg1	dysbiosis					1402:1410	gut dysbiosis	1398:1410	gut dysbiosis	1398:1410	In conclusion, quercetin is effective in recovering gut microbiota in mice after antibiotic treatment and may act as a prebiotic in combatting gut dysbiosis.
32845255	6	51	theme	P	807:807	arg1	mice					801:804	antibiotic-treated mice	782:804	antibiotic-treated mice (P < 0.05)	782:815	The results showed that quercetin supplementation significantly improved the diversity of the gut bacterial community in antibiotic-treated mice (P < 0.05).
32845255	6	51	theme	P	807:807	arg1	<					809:809	P < 0.05	807:814	P < 0.05	807:814	The results showed that quercetin supplementation significantly improved the diversity of the gut bacterial community in antibiotic-treated mice (P < 0.05).
32845255	4	52	theme	Gut	401:403	arg1	dysbiosis					405:413	Gut dysbiosis	401:413	Gut dysbiosis	401:413	Gut dysbiosis was successfully induced in mice by treatment with an antibiotic cocktail.
32845255	7	53	theme	diamine	978:984	arg1	oxidase					986:992	serum diamine oxidase	972:992	serum diamine oxidase (P < 0.05)	972:1003	Meanwhile, intestinal barrier function was also recovered remarkably as indicated by a decrease in the content of serum d-lactic acid and the activity of serum diamine oxidase (P < 0.05).
32845255	7	53	theme	diamine	978:984	arg1	<					997:997	P < 0.05	995:1002	P < 0.05	995:1002	Meanwhile, intestinal barrier function was also recovered remarkably as indicated by a decrease in the content of serum d-lactic acid and the activity of serum diamine oxidase (P < 0.05).
32845255	1	54	theme	gut	95:97	arg1	residing					110:117	the gut microbiota residing	91:117	the gut microbiota residing	91:117	The diversity and activity of the gut microbiota residing in humans and animals are significantly influenced by the diet.
32845255	8	55	theme	quercetin	1108:1116	arg1	treatment					1118:1126	quercetin treatment	1108:1126	quercetin treatment	1108:1126	The length of intestinal villi and mucosal thickness were also significantly increased in response to quercetin treatment (P < 0.05).
32845255	6	56	theme	gut	755:757	arg1	community					769:777	the gut bacterial community	751:777	the gut bacterial community	751:777	The results showed that quercetin supplementation significantly improved the diversity of the gut bacterial community in antibiotic-treated mice (P < 0.05).
32845255	6	57	theme	community	769:777	arg1	diversity					738:746	the diversity	734:746	the diversity of the gut bacterial community in antibiotic-treated mice (P < 0.05)	734:815	The results showed that quercetin supplementation significantly improved the diversity of the gut bacterial community in antibiotic-treated mice (P < 0.05).
32845255	1	58	dep	diversity	65:73	arg1	The					61:63	The	61:63	The	61:63	The diversity and activity of the gut microbiota residing in humans and animals are significantly influenced by the diet.
32845255	9	59	theme	butyrate	1171:1178	arg1	production					1157:1166	the production	1153:1166	the production of butyrate in faeces	1153:1188	Furthermore, the production of butyrate in faeces was enhanced significantly in quercetin-treated mice (P < 0.05).
32845255	8	60	theme	P	1129:1129	arg1	<					1131:1131	P < 0.05	1129:1136	P < 0.05	1129:1136	The length of intestinal villi and mucosal thickness were also significantly increased in response to quercetin treatment (P < 0.05).
32845255	1	61	from	diversity	65:73	arg1	humans					122:127	humans	122:127	humans	122:127	The diversity and activity of the gut microbiota residing in humans and animals are significantly influenced by the diet.
32845255	1	61	from	diversity	65:73	arg1	animals					133:139	animals	133:139	animals	133:139	The diversity and activity of the gut microbiota residing in humans and animals are significantly influenced by the diet.
32845255	10	62	from	effective	1283:1291	arg1	conclusion					1258:1267	conclusion	1258:1267	conclusion	1258:1267	In conclusion, quercetin is effective in recovering gut microbiota in mice after antibiotic treatment and may act as a prebiotic in combatting gut dysbiosis.
32845255	5	63	theme	16S	513:515	arg1	sequencing					538:547	16S rDNA high-throughput sequencing	513:547	16S rDNA high-throughput sequencing	513:547	Gas chromatography and 16S rDNA high-throughput sequencing techniques were used to investigate short-chain fatty acid content and gut microbial diversity and composition.
32845255	7	64	from	activity	960:967	arg1	content					921:927	the content	917:927	the content of serum d-lactic acid	917:950	Meanwhile, intestinal barrier function was also recovered remarkably as indicated by a decrease in the content of serum d-lactic acid and the activity of serum diamine oxidase (P < 0.05).
32845255	7	65	theme	serum	932:936	arg1	acid					947:950	serum d-lactic acid	932:950	serum d-lactic acid	932:950	Meanwhile, intestinal barrier function was also recovered remarkably as indicated by a decrease in the content of serum d-lactic acid and the activity of serum diamine oxidase (P < 0.05).
32845255	3	66	theme	study	313:317	arg1	aim					301:303	The aim	297:303	The aim of this study	297:317	The aim of this study was to investigate the prebiotic effects of quercetin in antibiotic-treated mice.
32845255	0	67	from	dysbiosis	23:31	arg1	mice					55:58	antibiotic-treated mice	36:58	antibiotic-treated mice	36:58	Quercetin improves gut dysbiosis in antibiotic-treated mice.
32845255	2	68	theme	human	235:239	arg1	diets					241:245	human diets	235:245	human diets	235:245	Quercetin, one of the representative polyphenols in human diets, possesses a wide range of biological properties.
32845255	5	69	theme	rDNA	517:520	arg1	sequencing					538:547	16S rDNA high-throughput sequencing	513:547	16S rDNA high-throughput sequencing	513:547	Gas chromatography and 16S rDNA high-throughput sequencing techniques were used to investigate short-chain fatty acid content and gut microbial diversity and composition.
32845255	10	70	from	conclusion	1258:1267	arg1	effective					1283:1291	effective	1283:1291	effective	1283:1291	In conclusion, quercetin is effective in recovering gut microbiota in mice after antibiotic treatment and may act as a prebiotic in combatting gut dysbiosis.
32845255	5	71	used	used	565:568	arg2	techniques					549:558	Gas chromatography and 16S rDNA high-throughput sequencing techniques	490:558	Gas chromatography and 16S rDNA high-throughput sequencing techniques	490:558	Gas chromatography and 16S rDNA high-throughput sequencing techniques were used to investigate short-chain fatty acid content and gut microbial diversity and composition.
31967953	5	0	theme	chemotaxonomic	519:532	arg1	analysis					534:541	the chemotaxonomic analysis	515:541	the chemotaxonomic analysis	515:541	The results of the chemotaxonomic analysis indicated that the predominant isoprenoid quinones were MK-8 and MK-9.
31967953	9	1	theme	name	1058:1061	arg1	sp					1088:1089	the name Nesterenkonia muleiensis sp	1054:1089	the name Nesterenkonia muleiensis sp	1054:1089	The results of the phylogenetic analysis, along with the phenotypic and chemotaxonomic characteristics, indicate that strain RB2T represents a novel species of the genus Nesterenkonia, for which the name Nesterenkonia muleiensis sp.
31967953	3	2	theme	optimum	419:425	arg1	NaCl					413:416	0-12 % (w/v) NaCl	400:416	0-12 % (w/v) NaCl (optimum 1 %)	400:430	RB2T was able to grow at 10-45 °C (optimum 35 °C), pH 6.0-12.0 (optimum 8.0) and with 0-12 % (w/v) NaCl (optimum 1 %).
31967953	3	2	theme	optimum	419:425	arg1	%					429:429	optimum 1 %	419:429	optimum 1 %	419:429	RB2T was able to grow at 10-45 °C (optimum 35 °C), pH 6.0-12.0 (optimum 8.0) and with 0-12 % (w/v) NaCl (optimum 1 %).
31967953	2	3	theme	novel	102:106	arg1	bacterium					188:196	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium	100:196	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated RB2T	100:212	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated RB2T was isolated from sap of Populus euphratica collected in Mulei county, Xinjiang province, PR China.
31967953	5	4	theme	analysis	534:541	arg1	results					504:510	The results	500:510	The results of the chemotaxonomic analysis	500:541	The results of the chemotaxonomic analysis indicated that the predominant isoprenoid quinones were MK-8 and MK-9.
31967953	9	5	theme	Nesterenkonia	1063:1075	arg1	sp					1088:1089	the name Nesterenkonia muleiensis sp	1054:1089	the name Nesterenkonia muleiensis sp	1054:1089	The results of the phylogenetic analysis, along with the phenotypic and chemotaxonomic characteristics, indicate that strain RB2T represents a novel species of the genus Nesterenkonia, for which the name Nesterenkonia muleiensis sp.
31967953	9	6	theme	muleiensis	1077:1086	arg1	sp					1088:1089	the name Nesterenkonia muleiensis sp	1054:1089	the name Nesterenkonia muleiensis sp	1054:1089	The results of the phylogenetic analysis, along with the phenotypic and chemotaxonomic characteristics, indicate that strain RB2T represents a novel species of the genus Nesterenkonia, for which the name Nesterenkonia muleiensis sp.
31967953	2	7	theme	Xinjiang	285:292	arg1	province					294:301	Mulei county, Xinjiang province	271:301	Mulei county, Xinjiang province	271:301	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated RB2T was isolated from sap of Populus euphratica collected in Mulei county, Xinjiang province, PR China.
31967953	2	7	theme	Xinjiang	285:292	arg1	China					307:311	PR China	304:311	PR China	304:311	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated RB2T was isolated from sap of Populus euphratica collected in Mulei county, Xinjiang province, PR China.
31967953	6	8	dep	anteiso-C15 	641:652	arg1	anteiso-C17 					661:672	anteiso-C17 	661:672	anteiso-C17 	661:672	The major fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0.
31967953	6	8	dep	anteiso-C15 	641:652	arg1	 0					654:655	 0	654:655	 0	654:655	The major fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0.
31967953	6	8	dep	anteiso-C15 	641:652	arg1	 0					674:675	 0	674:675	anteiso-C15 : 0 and anteiso-C17 : 0	641:675	The major fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0.
31967953	2	9	attach	isolated	218:225	arg1	sap					232:234	sap	232:234	sap of Populus euphratica collected in Mulei county, Xinjiang province, PR China	232:311	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated RB2T was isolated from sap of Populus euphratica collected in Mulei county, Xinjiang province, PR China.
31967953	2	9	attach	isolated	218:225	arg2	bacterium					188:196	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium	100:196	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated RB2T	100:212	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated RB2T was isolated from sap of Populus euphratica collected in Mulei county, Xinjiang province, PR China.
31967953	2	10	theme	non-endospore-forming	139:159	arg1	bacterium					188:196	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium	100:196	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated RB2T	100:212	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated RB2T was isolated from sap of Populus euphratica collected in Mulei county, Xinjiang province, PR China.
31967953	8	11	theme	peptidoglycan	806:818	arg1	type					820:823	The peptidoglycan type	802:823	The peptidoglycan type of RB2T	802:831	The peptidoglycan type of RB2T was A4α, l-Lys-Gly-l-Glu.
31967953	8	11	theme	peptidoglycan	806:818	arg1	A4α					837:839	A4α	837:839	A4α	837:839	The peptidoglycan type of RB2T was A4α, l-Lys-Gly-l-Glu.
31967953	7	12	theme	major	682:686	arg1	diphosphatidylglycerol					714:735	diphosphatidylglycerol	714:735	diphosphatidylglycerol	714:735	The major polar lipids of RB2T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two glycolipids.
31967953	7	12	theme	major	682:686	arg1	lipids					694:699	The major polar lipids	678:699	The major polar lipids of RB2T	678:707	The major polar lipids of RB2T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two glycolipids.
31967953	2	13	theme	aerobic	130:136	arg1	bacterium					188:196	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium	100:196	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated RB2T	100:212	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated RB2T was isolated from sap of Populus euphratica collected in Mulei county, Xinjiang province, PR China.
31967953	0	14	theme	muleiensis	14:23	arg1	sp					25:26	Nesterenkonia muleiensis sp	0:26	Nesterenkonia muleiensis sp.	0:27	Nesterenkonia muleiensis sp.
31967953	2	15	theme	PR	304:305	arg1	province					294:301	Mulei county, Xinjiang province	271:301	Mulei county, Xinjiang province	271:301	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated RB2T was isolated from sap of Populus euphratica collected in Mulei county, Xinjiang province, PR China.
31967953	2	15	theme	PR	304:305	arg1	China					307:311	PR China	304:311	PR China	304:311	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated RB2T was isolated from sap of Populus euphratica collected in Mulei county, Xinjiang province, PR China.
31967953	5	16	theme	predominant	562:572	arg1	MK-8					599:602	MK-8	599:602	MK-8	599:602	The results of the chemotaxonomic analysis indicated that the predominant isoprenoid quinones were MK-8 and MK-9.
31967953	5	16	theme	predominant	562:572	arg1	quinones					585:592	the predominant isoprenoid quinones	558:592	the predominant isoprenoid quinones	558:592	The results of the chemotaxonomic analysis indicated that the predominant isoprenoid quinones were MK-8 and MK-9.
31967953	0	17	theme	Nesterenkonia	0:12	arg1	sp					25:26	Nesterenkonia muleiensis sp	0:26	Nesterenkonia muleiensis sp.	0:27	Nesterenkonia muleiensis sp.
31967953	1	18	attach	isolated	59:66	arg2	actinobacterium					43:57	a novel actinobacterium	35:57	a novel actinobacterium isolated from sap of Populus euphratica	35:97	nov., a novel actinobacterium isolated from sap of Populus euphratica.
31967953	1	18	attach	isolated	59:66	arg1	sap					73:75	sap	73:75	sap of Populus euphratica	73:97	nov., a novel actinobacterium isolated from sap of Populus euphratica.
31967953	1	19	theme	Populus	80:86	arg1	euphratica					88:97	Populus euphratica	80:97	Populus euphratica	80:97	nov., a novel actinobacterium isolated from sap of Populus euphratica.
31967953	4	20	theme	G+C	449:451	arg1	content					453:459	The genomic DNA G+C content	433:459	The genomic DNA G+C content	433:459	The genomic DNA G+C content was 63.5 % (from the genome sequence).
31967953	4	20	theme	G+C	449:451	arg1	%					470:470	63.5 %	465:470	63.5 %	465:470	The genomic DNA G+C content was 63.5 % (from the genome sequence).
31967953	1	21	theme	euphratica	88:97	arg1	sap					73:75	sap	73:75	sap of Populus euphratica	73:97	nov., a novel actinobacterium isolated from sap of Populus euphratica.
31967953	7	22	theme	RB2T	704:707	arg1	diphosphatidylglycerol					714:735	diphosphatidylglycerol	714:735	diphosphatidylglycerol	714:735	The major polar lipids of RB2T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two glycolipids.
31967953	7	22	theme	RB2T	704:707	arg1	lipids					694:699	The major polar lipids	678:699	The major polar lipids of RB2T	678:707	The major polar lipids of RB2T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two glycolipids.
31967953	3	23	theme	%	405:405	arg1	NaCl					413:416	0-12 % (w/v) NaCl	400:416	0-12 % (w/v) NaCl (optimum 1 %)	400:430	RB2T was able to grow at 10-45 °C (optimum 35 °C), pH 6.0-12.0 (optimum 8.0) and with 0-12 % (w/v) NaCl (optimum 1 %).
31967953	3	23	theme	%	405:405	arg1	%					429:429	optimum 1 %	419:429	optimum 1 %	419:429	RB2T was able to grow at 10-45 °C (optimum 35 °C), pH 6.0-12.0 (optimum 8.0) and with 0-12 % (w/v) NaCl (optimum 1 %).
31967953	3	24	theme	optimum	349:355	arg1	35 °C					357:361	optimum 35 °C	349:361	optimum 35 °C	349:361	RB2T was able to grow at 10-45 °C (optimum 35 °C), pH 6.0-12.0 (optimum 8.0) and with 0-12 % (w/v) NaCl (optimum 1 %).
31967953	3	24	theme	optimum	349:355	arg1	10-45 °C					339:346	10-45 °C	339:346	10-45 °C (optimum 35 °C)	339:362	RB2T was able to grow at 10-45 °C (optimum 35 °C), pH 6.0-12.0 (optimum 8.0) and with 0-12 % (w/v) NaCl (optimum 1 %).
31967953	2	25	theme	rod-shaped	177:186	arg1	bacterium					188:196	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium	100:196	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated RB2T	100:212	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated RB2T was isolated from sap of Populus euphratica collected in Mulei county, Xinjiang province, PR China.
31967953	7	26	theme	polar	688:692	arg1	diphosphatidylglycerol					714:735	diphosphatidylglycerol	714:735	diphosphatidylglycerol	714:735	The major polar lipids of RB2T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two glycolipids.
31967953	7	26	theme	polar	688:692	arg1	lipids					694:699	The major polar lipids	678:699	The major polar lipids of RB2T	678:707	The major polar lipids of RB2T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two glycolipids.
31967953	9	27	theme	phylogenetic	878:889	arg1	analysis					891:898	the phylogenetic analysis	874:898	the phylogenetic analysis	874:898	The results of the phylogenetic analysis, along with the phenotypic and chemotaxonomic characteristics, indicate that strain RB2T represents a novel species of the genus Nesterenkonia, for which the name Nesterenkonia muleiensis sp.
31967953	9	28	theme	strain	977:982	arg1	RB2T					984:987	strain RB2T	977:987	strain RB2T	977:987	The results of the phylogenetic analysis, along with the phenotypic and chemotaxonomic characteristics, indicate that strain RB2T represents a novel species of the genus Nesterenkonia, for which the name Nesterenkonia muleiensis sp.
31967953	11	29	theme	1K03528T=KCTC	1141:1153	arg1	49017T					1155:1160	=MCCC 1K03528T=KCTC 49017T	1135:1160	=MCCC 1K03528T=KCTC 49017T	1135:1160	The type strain is RB2T (=MCCC 1K03528T=KCTC 49017T).
31967953	11	29	theme	1K03528T=KCTC	1141:1153	arg1	RB2T					1129:1132	RB2T	1129:1132	RB2T (=MCCC 1K03528T=KCTC 49017T)	1129:1161	The type strain is RB2T (=MCCC 1K03528T=KCTC 49017T).
31967953	9	30	theme	analysis	891:898	arg1	results					863:869	The results	859:869	The results of the phylogenetic analysis	859:898	The results of the phylogenetic analysis, along with the phenotypic and chemotaxonomic characteristics, indicate that strain RB2T represents a novel species of the genus Nesterenkonia, for which the name Nesterenkonia muleiensis sp.
31967953	11	31	theme	type	1114:1117	arg1	strain					1119:1124	The type strain	1110:1124	The type strain	1110:1124	The type strain is RB2T (=MCCC 1K03528T=KCTC 49017T).
31967953	11	31	theme	type	1114:1117	arg1	RB2T					1129:1132	RB2T	1129:1132	RB2T (=MCCC 1K03528T=KCTC 49017T)	1129:1161	The type strain is RB2T (=MCCC 1K03528T=KCTC 49017T).
31967953	2	32	theme	non-motile	162:171	arg1	bacterium					188:196	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium	100:196	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated RB2T	100:212	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated RB2T was isolated from sap of Populus euphratica collected in Mulei county, Xinjiang province, PR China.
31967953	11	33	theme	=MCCC	1135:1139	arg1	49017T					1155:1160	=MCCC 1K03528T=KCTC 49017T	1135:1160	=MCCC 1K03528T=KCTC 49017T	1135:1160	The type strain is RB2T (=MCCC 1K03528T=KCTC 49017T).
31967953	11	33	theme	=MCCC	1135:1139	arg1	RB2T					1129:1132	RB2T	1129:1132	RB2T (=MCCC 1K03528T=KCTC 49017T)	1129:1161	The type strain is RB2T (=MCCC 1K03528T=KCTC 49017T).
31967953	4	34	dep	%	470:470	arg1	sequence					489:496	the genome sequence	478:496	the genome sequence	478:496	The genomic DNA G+C content was 63.5 % (from the genome sequence).
31967953	9	35	theme	genus	1023:1027	arg1	Nesterenkonia					1029:1041	the genus Nesterenkonia	1019:1041	the genus Nesterenkonia	1019:1041	The results of the phylogenetic analysis, along with the phenotypic and chemotaxonomic characteristics, indicate that strain RB2T represents a novel species of the genus Nesterenkonia, for which the name Nesterenkonia muleiensis sp.
31967953	4	36	theme	DNA	445:447	arg1	content					453:459	The genomic DNA G+C content	433:459	The genomic DNA G+C content	433:459	The genomic DNA G+C content was 63.5 % (from the genome sequence).
31967953	4	36	theme	DNA	445:447	arg1	%					470:470	63.5 %	465:470	63.5 %	465:470	The genomic DNA G+C content was 63.5 % (from the genome sequence).
31967953	3	37	theme	w/v	408:410	arg1	NaCl					413:416	0-12 % (w/v) NaCl	400:416	0-12 % (w/v) NaCl (optimum 1 %)	400:430	RB2T was able to grow at 10-45 °C (optimum 35 °C), pH 6.0-12.0 (optimum 8.0) and with 0-12 % (w/v) NaCl (optimum 1 %).
31967953	3	37	theme	w/v	408:410	arg1	%					429:429	optimum 1 %	419:429	optimum 1 %	419:429	RB2T was able to grow at 10-45 °C (optimum 35 °C), pH 6.0-12.0 (optimum 8.0) and with 0-12 % (w/v) NaCl (optimum 1 %).
31967953	9	38	theme	Nesterenkonia	1029:1041	arg1	species					1008:1014	a novel species	1000:1014	a novel species	1000:1014	The results of the phylogenetic analysis, along with the phenotypic and chemotaxonomic characteristics, indicate that strain RB2T represents a novel species of the genus Nesterenkonia, for which the name Nesterenkonia muleiensis sp.
31967953	4	39	theme	genomic	437:443	arg1	content					453:459	The genomic DNA G+C content	433:459	The genomic DNA G+C content	433:459	The genomic DNA G+C content was 63.5 % (from the genome sequence).
31967953	4	39	theme	genomic	437:443	arg1	%					470:470	63.5 %	465:470	63.5 %	465:470	The genomic DNA G+C content was 63.5 % (from the genome sequence).
31967953	5	40	theme	isoprenoid	574:583	arg1	MK-8					599:602	MK-8	599:602	MK-8	599:602	The results of the chemotaxonomic analysis indicated that the predominant isoprenoid quinones were MK-8 and MK-9.
31967953	5	40	theme	isoprenoid	574:583	arg1	quinones					585:592	the predominant isoprenoid quinones	558:592	the predominant isoprenoid quinones	558:592	The results of the chemotaxonomic analysis indicated that the predominant isoprenoid quinones were MK-8 and MK-9.
31967953	8	41	theme	RB2T	828:831	arg1	type					820:823	The peptidoglycan type	802:823	The peptidoglycan type of RB2T	802:831	The peptidoglycan type of RB2T was A4α, l-Lys-Gly-l-Glu.
31967953	8	41	theme	RB2T	828:831	arg1	A4α					837:839	A4α	837:839	A4α	837:839	The peptidoglycan type of RB2T was A4α, l-Lys-Gly-l-Glu.
31967953	1	42	dep	actinobacterium	43:57	arg1	nov.					29:32	nov.	29:32	nov.	29:32	nov., a novel actinobacterium isolated from sap of Populus euphratica.
31967953	6	43	theme	fatty	624:628	arg1	anteiso-C15 					641:652	anteiso-C15 	641:652	anteiso-C15 	641:652	The major fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0.
31967953	6	43	theme	fatty	624:628	arg1	acids					630:634	The major fatty acids	614:634	The major fatty acids	614:634	The major fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0.
31967953	9	44	theme	novel	1002:1006	arg1	species					1008:1014	a novel species	1000:1014	a novel species	1000:1014	The results of the phylogenetic analysis, along with the phenotypic and chemotaxonomic characteristics, indicate that strain RB2T represents a novel species of the genus Nesterenkonia, for which the name Nesterenkonia muleiensis sp.
31967953	9	45	theme	phenotypic	916:925	arg1	characteristics					946:960	the phenotypic and chemotaxonomic characteristics	912:960	the phenotypic and chemotaxonomic characteristics	912:960	The results of the phylogenetic analysis, along with the phenotypic and chemotaxonomic characteristics, indicate that strain RB2T represents a novel species of the genus Nesterenkonia, for which the name Nesterenkonia muleiensis sp.
31967953	4	46	theme	genome	482:487	arg1	sequence					489:496	the genome sequence	478:496	the genome sequence	478:496	The genomic DNA G+C content was 63.5 % (from the genome sequence).
31967953	2	47	theme	euphratica	247:256	arg1	sap					232:234	sap	232:234	sap of Populus euphratica collected in Mulei county, Xinjiang province, PR China	232:311	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated RB2T was isolated from sap of Populus euphratica collected in Mulei county, Xinjiang province, PR China.
31967953	6	48	theme	major	618:622	arg1	anteiso-C15 					641:652	anteiso-C15 	641:652	anteiso-C15 	641:652	The major fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0.
31967953	6	48	theme	major	618:622	arg1	acids					630:634	The major fatty acids	614:634	The major fatty acids	614:634	The major fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0.
31967953	8	49	dep	A4α	837:839	arg1	l-Lys-Gly-l-Glu					842:856	l-Lys-Gly-l-Glu	842:856	l-Lys-Gly-l-Glu	842:856	The peptidoglycan type of RB2T was A4α, l-Lys-Gly-l-Glu.
31967953	2	50	theme	Gram-stain-positive	109:127	arg1	bacterium					188:196	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium	100:196	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated RB2T	100:212	A novel, Gram-stain-positive, aerobic, non-endospore-forming, non-motile and rod-shaped bacterium designated RB2T was isolated from sap of Populus euphratica collected in Mulei county, Xinjiang province, PR China.
31967953	1	51	theme	novel	37:41	arg1	actinobacterium					43:57	a novel actinobacterium	35:57	a novel actinobacterium isolated from sap of Populus euphratica	35:97	nov., a novel actinobacterium isolated from sap of Populus euphratica.
31967953	9	52	theme	chemotaxonomic	931:944	arg1	characteristics					946:960	the phenotypic and chemotaxonomic characteristics	912:960	the phenotypic and chemotaxonomic characteristics	912:960	The results of the phylogenetic analysis, along with the phenotypic and chemotaxonomic characteristics, indicate that strain RB2T represents a novel species of the genus Nesterenkonia, for which the name Nesterenkonia muleiensis sp.
31702538	9	0	theme	15747T=JCM	1215:1224	arg1	32769T					1226:1231	=KCTC 15747T=JCM 32769T	1209:1231	=KCTC 15747T=JCM 32769T	1209:1231	nov., is proposed for isolate YH-panp20T (=KCTC 15747T=JCM 32769T).
31702538	9	0	theme	15747T=JCM	1215:1224	arg1	YH-panp20T					1197:1206	isolate YH-panp20T	1189:1206	isolate YH-panp20T (=KCTC 15747T=JCM 32769T)	1189:1232	nov., is proposed for isolate YH-panp20T (=KCTC 15747T=JCM 32769T).
31702538	3	1	theme	Faecalitalea	483:494	arg1	%					526:526	92.2 %	521:526	92.2 %	521:526	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	3	1	theme	Faecalitalea	483:494	arg1	5815T					514:518	Faecalitalea cylindroides KCTC 5815T	483:518	Faecalitalea cylindroides KCTC 5815T (92.2 %)	483:527	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	2	2	attach	isolated	215:222	arg1	faeces					233:238	pig faeces	229:238	pig faeces	229:238	An obligately anaerobic, Gram-stain-positive and coccus-shaped bacterium, designated strain YH-panp20T, was isolated from pig faeces.
31702538	2	2	attach	isolated	215:222	arg2	bacterium					170:178	An obligately anaerobic, Gram-stain-positive and coccus-shaped bacterium	107:178	An obligately anaerobic, Gram-stain-positive and coccus-shaped bacterium	107:178	An obligately anaerobic, Gram-stain-positive and coccus-shaped bacterium, designated strain YH-panp20T, was isolated from pig faeces.
31702538	7	3	theme	glucose	972:978	arg1	fermentation					980:991	glucose fermentation	972:991	glucose fermentation	972:991	The major end-products of glucose fermentation were lactate, acetate and formate.
31702538	3	4	theme	cylindroides	496:507	arg1	%					526:526	92.2 %	521:526	92.2 %	521:526	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	3	4	theme	cylindroides	496:507	arg1	5815T					514:518	Faecalitalea cylindroides KCTC 5815T	483:518	Faecalitalea cylindroides KCTC 5815T (92.2 %)	483:527	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	3	5	theme	family	339:344	arg1	Erysipelotrichaceae					346:364	the family Erysipelotrichaceae	335:364	the family Erysipelotrichaceae	335:364	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	5	6	theme	A1γ	824:826	arg1	type					828:831	A1γ type	824:831	A1γ type	824:831	The G+C content of the isolate was 38.4 mol%, and its cell-wall peptidoglycan was found to be of A1γ type and contained meso-diaminopimelic acid.
31702538	8	7	theme	phylogenetic	1064:1075	arg1	species					1126:1132	species	1126:1132	species	1126:1132	Therefore, based on the phenotypic, phylogenetic and chemotaxonomic properties, a novel genus and species, Absicoccus porci gen. nov., sp.
31702538	8	7	theme	phylogenetic	1064:1075	arg1	genus					1116:1120	a novel genus	1108:1120	a novel genus	1108:1120	Therefore, based on the phenotypic, phylogenetic and chemotaxonomic properties, a novel genus and species, Absicoccus porci gen. nov., sp.
31702538	8	7	theme	phylogenetic	1064:1075	arg1	properties					1096:1105	the phenotypic, phylogenetic and chemotaxonomic properties	1048:1105	the phenotypic, phylogenetic and chemotaxonomic properties	1048:1105	Therefore, based on the phenotypic, phylogenetic and chemotaxonomic properties, a novel genus and species, Absicoccus porci gen. nov., sp.
31702538	8	8	theme	phenotypic	1052:1061	arg1	species					1126:1132	species	1126:1132	species	1126:1132	Therefore, based on the phenotypic, phylogenetic and chemotaxonomic properties, a novel genus and species, Absicoccus porci gen. nov., sp.
31702538	8	8	theme	phenotypic	1052:1061	arg1	genus					1116:1120	a novel genus	1108:1120	a novel genus	1108:1120	Therefore, based on the phenotypic, phylogenetic and chemotaxonomic properties, a novel genus and species, Absicoccus porci gen. nov., sp.
31702538	8	8	theme	phenotypic	1052:1061	arg1	properties					1096:1105	the phenotypic, phylogenetic and chemotaxonomic properties	1048:1105	the phenotypic, phylogenetic and chemotaxonomic properties	1048:1105	Therefore, based on the phenotypic, phylogenetic and chemotaxonomic properties, a novel genus and species, Absicoccus porci gen. nov., sp.
31702538	2	9	theme	anaerobic	121:129	arg1	bacterium					170:178	An obligately anaerobic, Gram-stain-positive and coccus-shaped bacterium	107:178	An obligately anaerobic, Gram-stain-positive and coccus-shaped bacterium	107:178	An obligately anaerobic, Gram-stain-positive and coccus-shaped bacterium, designated strain YH-panp20T, was isolated from pig faeces.
31702538	7	10	theme	fermentation	980:991	arg1	lactate					998:1004	lactate	998:1004	lactate	998:1004	The major end-products of glucose fermentation were lactate, acetate and formate.
31702538	7	10	theme	fermentation	980:991	arg1	end-products					956:967	The major end-products	946:967	The major end-products of glucose fermentation	946:991	The major end-products of glucose fermentation were lactate, acetate and formate.
31702538	5	11	theme	isolate	750:756	arg1	content					735:741	The G+C content	727:741	The G+C content of the isolate	727:756	The G+C content of the isolate was 38.4 mol%, and its cell-wall peptidoglycan was found to be of A1γ type and contained meso-diaminopimelic acid.
31702538	5	11	theme	isolate	750:756	arg1	%					770:770	38.4 mol%	762:770	38.4 mol%	762:770	The G+C content of the isolate was 38.4 mol%, and its cell-wall peptidoglycan was found to be of A1γ type and contained meso-diaminopimelic acid.
31702538	3	12	theme	Phylogenetic	241:252	arg1	analysis					254:261	Phylogenetic analysis	241:261	Phylogenetic analysis based on 16S rRNA gene sequencing	241:295	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	6	13	dep	C18 	906:909	arg1	cis					914:916	 1 cis 9	911:918	C18 : 1 cis 9	906:918	The predominant fatty acids were C18 : 1 cis 9, C18 : 0 DMA and C16 : 0.
31702538	0	14	theme	porci	11:15	arg1	nov.					22:25	Absicoccus porci gen. nov.	0:25	Absicoccus porci gen. nov.	0:25	Absicoccus porci gen. nov., sp.
31702538	6	15	dep	C18 	921:924	arg1	DMA					929:931	 0 DMA	926:931	C18 : 0 DMA	921:931	The predominant fatty acids were C18 : 1 cis 9, C18 : 0 DMA and C16 : 0.
31702538	4	16	theme	Average	625:631	arg1	values					653:658	Average nucleotide identity values	625:658	Average nucleotide identity values between YH-panp20T and its closest relatives	625:703	Average nucleotide identity values between YH-panp20T and its closest relatives were lower than 71 %.
31702538	0	17	theme	Absicoccus	0:9	arg1	nov.					22:25	Absicoccus porci gen. nov.	0:25	Absicoccus porci gen. nov.	0:25	Absicoccus porci gen. nov., sp.
31702538	9	18	theme	isolate	1189:1195	arg1	32769T					1226:1231	=KCTC 15747T=JCM 32769T	1209:1231	=KCTC 15747T=JCM 32769T	1209:1231	nov., is proposed for isolate YH-panp20T (=KCTC 15747T=JCM 32769T).
31702538	9	18	theme	isolate	1189:1195	arg1	YH-panp20T					1197:1206	isolate YH-panp20T	1189:1206	isolate YH-panp20T (=KCTC 15747T=JCM 32769T)	1189:1232	nov., is proposed for isolate YH-panp20T (=KCTC 15747T=JCM 32769T).
31702538	3	19	dep	Catenisphaera	398:410	arg1	adipataccumulans					412:427	adipataccumulans	412:427	adipataccumulans	412:427	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	1	20	theme	family	54:59	arg1	Erysipelotrichaceae					61:79	the family Erysipelotrichaceae	50:79	the family Erysipelotrichaceae isolated from pig faeces	50:104	nov., a member of the family Erysipelotrichaceae isolated from pig faeces.
31702538	8	21	theme	Absicoccus	1135:1144	arg1	genus					1116:1120	a novel genus	1108:1120	a novel genus	1108:1120	Therefore, based on the phenotypic, phylogenetic and chemotaxonomic properties, a novel genus and species, Absicoccus porci gen. nov., sp.
31702538	8	21	theme	Absicoccus	1135:1144	arg1	nov.					1157:1160	Absicoccus porci gen. nov.	1135:1160	Absicoccus porci gen. nov.	1135:1160	Therefore, based on the phenotypic, phylogenetic and chemotaxonomic properties, a novel genus and species, Absicoccus porci gen. nov., sp.
31702538	3	22	theme	KCTC	429:432	arg1	15517T					434:439	Catenisphaera adipataccumulans KCTC 15517T	398:439	Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity)	398:468	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	3	22	theme	KCTC	429:432	arg1	similarity					458:467	93.5 % sequence similarity	442:467	93.5 % sequence similarity	442:467	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	5	23	theme	38.4 mol	762:769	arg1	content					735:741	The G+C content	727:741	The G+C content of the isolate	727:756	The G+C content of the isolate was 38.4 mol%, and its cell-wall peptidoglycan was found to be of A1γ type and contained meso-diaminopimelic acid.
31702538	5	23	theme	38.4 mol	762:769	arg1	%					770:770	38.4 mol%	762:770	38.4 mol%	762:770	The G+C content of the isolate was 38.4 mol%, and its cell-wall peptidoglycan was found to be of A1γ type and contained meso-diaminopimelic acid.
31702538	7	24	theme	major	950:954	arg1	lactate					998:1004	lactate	998:1004	lactate	998:1004	The major end-products of glucose fermentation were lactate, acetate and formate.
31702538	7	24	theme	major	950:954	arg1	end-products					956:967	The major end-products	946:967	The major end-products of glucose fermentation	946:991	The major end-products of glucose fermentation were lactate, acetate and formate.
31702538	1	25	theme	Erysipelotrichaceae	61:79	arg1	member					40:45	a member	38:45	a member of the family Erysipelotrichaceae isolated from pig faeces	38:104	nov., a member of the family Erysipelotrichaceae isolated from pig faeces.
31702538	1	25	theme	Erysipelotrichaceae	61:79	arg1	nov.					32:35	nov.	32:35	nov.	32:35	nov., a member of the family Erysipelotrichaceae isolated from pig faeces.
31702538	3	26	theme	sequence	449:456	arg1	15517T					434:439	Catenisphaera adipataccumulans KCTC 15517T	398:439	Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity)	398:468	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	3	26	theme	sequence	449:456	arg1	similarity					458:467	93.5 % sequence similarity	442:467	93.5 % sequence similarity	442:467	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	0	27	theme	gen.	17:20	arg1	nov.					22:25	Absicoccus porci gen. nov.	0:25	Absicoccus porci gen. nov.	0:25	Absicoccus porci gen. nov., sp.
31702538	3	28	theme	16S	272:274	arg1	sequencing					286:295	16S rRNA gene sequencing	272:295	16S rRNA gene sequencing	272:295	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	3	29	theme	Faecalicoccus	530:542	arg1	15521T					562:567	Faecalicoccus acidiformans KCTC 15521T	530:567	Faecalicoccus acidiformans KCTC 15521T (90.2 %)	530:576	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	3	29	theme	Faecalicoccus	530:542	arg1	%					575:575	90.2 %	570:575	90.2 %	570:575	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	4	30	theme	identity	644:651	arg1	values					653:658	Average nucleotide identity values	625:658	Average nucleotide identity values between YH-panp20T and its closest relatives	625:703	Average nucleotide identity values between YH-panp20T and its closest relatives were lower than 71 %.
31702538	5	31	theme	G+C	731:733	arg1	content					735:741	The G+C content	727:741	The G+C content of the isolate	727:756	The G+C content of the isolate was 38.4 mol%, and its cell-wall peptidoglycan was found to be of A1γ type and contained meso-diaminopimelic acid.
31702538	5	31	theme	G+C	731:733	arg1	%					770:770	38.4 mol%	762:770	38.4 mol%	762:770	The G+C content of the isolate was 38.4 mol%, and its cell-wall peptidoglycan was found to be of A1γ type and contained meso-diaminopimelic acid.
31702538	9	32	theme	=KCTC	1209:1213	arg1	32769T					1226:1231	=KCTC 15747T=JCM 32769T	1209:1231	=KCTC 15747T=JCM 32769T	1209:1231	nov., is proposed for isolate YH-panp20T (=KCTC 15747T=JCM 32769T).
31702538	9	32	theme	=KCTC	1209:1213	arg1	YH-panp20T					1197:1206	isolate YH-panp20T	1189:1206	isolate YH-panp20T (=KCTC 15747T=JCM 32769T)	1189:1232	nov., is proposed for isolate YH-panp20T (=KCTC 15747T=JCM 32769T).
31702538	2	33	theme	coccus-shaped	156:168	arg1	bacterium					170:178	An obligately anaerobic, Gram-stain-positive and coccus-shaped bacterium	107:178	An obligately anaerobic, Gram-stain-positive and coccus-shaped bacterium	107:178	An obligately anaerobic, Gram-stain-positive and coccus-shaped bacterium, designated strain YH-panp20T, was isolated from pig faeces.
31702538	3	34	theme	Catenisphaera	398:410	arg1	15517T					434:439	Catenisphaera adipataccumulans KCTC 15517T	398:439	Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity)	398:468	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	3	34	theme	Catenisphaera	398:410	arg1	similarity					458:467	93.5 % sequence similarity	442:467	93.5 % sequence similarity	442:467	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	6	35	theme	fatty	889:893	arg1	C18 					906:909	C18 	906:909	C18 	906:909	The predominant fatty acids were C18 : 1 cis 9, C18 : 0 DMA and C16 : 0.
31702538	6	35	theme	fatty	889:893	arg1	acids					895:899	The predominant fatty acids	873:899	The predominant fatty acids	873:899	The predominant fatty acids were C18 : 1 cis 9, C18 : 0 DMA and C16 : 0.
31702538	3	36	theme	KCTC	604:607	arg1	5969T					609:613	Holdemanella biformis KCTC 5969T	582:613	Holdemanella biformis KCTC 5969T (89.6 %)	582:622	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	3	36	theme	KCTC	604:607	arg1	%					621:621	89.6 %	616:621	89.6 %	616:621	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	8	37	theme	chemotaxonomic	1081:1094	arg1	species					1126:1132	species	1126:1132	species	1126:1132	Therefore, based on the phenotypic, phylogenetic and chemotaxonomic properties, a novel genus and species, Absicoccus porci gen. nov., sp.
31702538	8	37	theme	chemotaxonomic	1081:1094	arg1	genus					1116:1120	a novel genus	1108:1120	a novel genus	1108:1120	Therefore, based on the phenotypic, phylogenetic and chemotaxonomic properties, a novel genus and species, Absicoccus porci gen. nov., sp.
31702538	8	37	theme	chemotaxonomic	1081:1094	arg1	properties					1096:1105	the phenotypic, phylogenetic and chemotaxonomic properties	1048:1105	the phenotypic, phylogenetic and chemotaxonomic properties	1048:1105	Therefore, based on the phenotypic, phylogenetic and chemotaxonomic properties, a novel genus and species, Absicoccus porci gen. nov., sp.
31702538	3	38	theme	%	447:447	arg1	15517T					434:439	Catenisphaera adipataccumulans KCTC 15517T	398:439	Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity)	398:468	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	3	38	theme	%	447:447	arg1	similarity					458:467	93.5 % sequence similarity	442:467	93.5 % sequence similarity	442:467	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	8	39	theme	novel	1110:1114	arg1	genus					1116:1120	a novel genus	1108:1120	a novel genus	1108:1120	Therefore, based on the phenotypic, phylogenetic and chemotaxonomic properties, a novel genus and species, Absicoccus porci gen. nov., sp.
31702538	8	39	theme	novel	1110:1114	arg1	properties					1096:1105	the phenotypic, phylogenetic and chemotaxonomic properties	1048:1105	the phenotypic, phylogenetic and chemotaxonomic properties	1048:1105	Therefore, based on the phenotypic, phylogenetic and chemotaxonomic properties, a novel genus and species, Absicoccus porci gen. nov., sp.
31702538	8	39	theme	novel	1110:1114	arg1	nov.					1157:1160	Absicoccus porci gen. nov.	1135:1160	Absicoccus porci gen. nov.	1135:1160	Therefore, based on the phenotypic, phylogenetic and chemotaxonomic properties, a novel genus and species, Absicoccus porci gen. nov., sp.
31702538	6	40	theme	predominant	877:887	arg1	C18 					906:909	C18 	906:909	C18 	906:909	The predominant fatty acids were C18 : 1 cis 9, C18 : 0 DMA and C16 : 0.
31702538	6	40	theme	predominant	877:887	arg1	acids					895:899	The predominant fatty acids	873:899	The predominant fatty acids	873:899	The predominant fatty acids were C18 : 1 cis 9, C18 : 0 DMA and C16 : 0.
31702538	2	41	theme	Gram-stain-positive	132:150	arg1	bacterium					170:178	An obligately anaerobic, Gram-stain-positive and coccus-shaped bacterium	107:178	An obligately anaerobic, Gram-stain-positive and coccus-shaped bacterium	107:178	An obligately anaerobic, Gram-stain-positive and coccus-shaped bacterium, designated strain YH-panp20T, was isolated from pig faeces.
31702538	0	42	dep	sp	28:29	arg1	nov.					22:25	Absicoccus porci gen. nov.	0:25	Absicoccus porci gen. nov.	0:25	Absicoccus porci gen. nov., sp.
31702538	1	43	attach	isolated	81:88	arg1	faeces					99:104	pig faeces	95:104	pig faeces	95:104	nov., a member of the family Erysipelotrichaceae isolated from pig faeces.
31702538	1	43	attach	isolated	81:88	arg2	Erysipelotrichaceae					61:79	the family Erysipelotrichaceae	50:79	the family Erysipelotrichaceae isolated from pig faeces	50:104	nov., a member of the family Erysipelotrichaceae isolated from pig faeces.
31702538	3	44	dep	related	387:393	arg1	followed					471:478	followed	471:478	followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %)	471:622	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	3	44	dep	related	387:393	arg1	isolate					316:322	the isolate	312:322	the isolate	312:322	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	3	44	dep	related	387:393	arg1	related					387:393	related	387:393	related	387:393	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	1	45	theme	pig	95:97	arg1	faeces					99:104	pig faeces	95:104	pig faeces	95:104	nov., a member of the family Erysipelotrichaceae isolated from pig faeces.
31702538	3	46	theme	rRNA	276:279	arg1	sequencing					286:295	16S rRNA gene sequencing	272:295	16S rRNA gene sequencing	272:295	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	2	47	theme	strain	192:197	arg1	YH-panp20T					199:208	strain YH-panp20T	192:208	strain YH-panp20T	192:208	An obligately anaerobic, Gram-stain-positive and coccus-shaped bacterium, designated strain YH-panp20T, was isolated from pig faeces.
31702538	3	48	theme	Holdemanella	582:593	arg1	5969T					609:613	Holdemanella biformis KCTC 5969T	582:613	Holdemanella biformis KCTC 5969T (89.6 %)	582:622	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	3	48	theme	Holdemanella	582:593	arg1	%					621:621	89.6 %	616:621	89.6 %	616:621	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	4	49	theme	nucleotide	633:642	arg1	values					653:658	Average nucleotide identity values	625:658	Average nucleotide identity values between YH-panp20T and its closest relatives	625:703	Average nucleotide identity values between YH-panp20T and its closest relatives were lower than 71 %.
31702538	4	50	theme	closest	687:693	arg1	relatives					695:703	its closest relatives	683:703	its closest relatives	683:703	Average nucleotide identity values between YH-panp20T and its closest relatives were lower than 71 %.
31702538	8	51	theme	gen.	1152:1155	arg1	genus					1116:1120	a novel genus	1108:1120	a novel genus	1108:1120	Therefore, based on the phenotypic, phylogenetic and chemotaxonomic properties, a novel genus and species, Absicoccus porci gen. nov., sp.
31702538	8	51	theme	gen.	1152:1155	arg1	nov.					1157:1160	Absicoccus porci gen. nov.	1135:1160	Absicoccus porci gen. nov.	1135:1160	Therefore, based on the phenotypic, phylogenetic and chemotaxonomic properties, a novel genus and species, Absicoccus porci gen. nov., sp.
31702538	3	52	theme	gene	281:284	arg1	sequencing					286:295	16S rRNA gene sequencing	272:295	16S rRNA gene sequencing	272:295	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	3	53	theme	biformis	595:602	arg1	5969T					609:613	Holdemanella biformis KCTC 5969T	582:613	Holdemanella biformis KCTC 5969T (89.6 %)	582:622	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	3	53	theme	biformis	595:602	arg1	%					621:621	89.6 %	616:621	89.6 %	616:621	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	3	54	dep	Faecalicoccus	530:542	arg1	acidiformans					544:555	acidiformans	544:555	acidiformans	544:555	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	5	55	theme	meso-diaminopimelic	847:865	arg1	acid					867:870	meso-diaminopimelic acid	847:870	meso-diaminopimelic acid	847:870	The G+C content of the isolate was 38.4 mol%, and its cell-wall peptidoglycan was found to be of A1γ type and contained meso-diaminopimelic acid.
31702538	5	56	theme	cell-wall	781:789	arg1	peptidoglycan					791:803	its cell-wall peptidoglycan	777:803	its cell-wall peptidoglycan	777:803	The G+C content of the isolate was 38.4 mol%, and its cell-wall peptidoglycan was found to be of A1γ type and contained meso-diaminopimelic acid.
31702538	8	57	theme	porci	1146:1150	arg1	genus					1116:1120	a novel genus	1108:1120	a novel genus	1108:1120	Therefore, based on the phenotypic, phylogenetic and chemotaxonomic properties, a novel genus and species, Absicoccus porci gen. nov., sp.
31702538	8	57	theme	porci	1146:1150	arg1	nov.					1157:1160	Absicoccus porci gen. nov.	1135:1160	Absicoccus porci gen. nov.	1135:1160	Therefore, based on the phenotypic, phylogenetic and chemotaxonomic properties, a novel genus and species, Absicoccus porci gen. nov., sp.
31702538	3	58	theme	KCTC	509:512	arg1	%					526:526	92.2 %	521:526	92.2 %	521:526	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	3	58	theme	KCTC	509:512	arg1	5815T					514:518	Faecalitalea cylindroides KCTC 5815T	483:518	Faecalitalea cylindroides KCTC 5815T (92.2 %)	483:527	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	2	59	theme	pig	229:231	arg1	faeces					233:238	pig faeces	229:238	pig faeces	229:238	An obligately anaerobic, Gram-stain-positive and coccus-shaped bacterium, designated strain YH-panp20T, was isolated from pig faeces.
31702538	3	60	theme	KCTC	557:560	arg1	15521T					562:567	Faecalicoccus acidiformans KCTC 15521T	530:567	Faecalicoccus acidiformans KCTC 15521T (90.2 %)	530:576	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
31702538	3	60	theme	KCTC	557:560	arg1	%					575:575	90.2 %	570:575	90.2 %	570:575	Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family Erysipelotrichaceae, and is most closely related to Catenisphaera adipataccumulans KCTC 15517T (93.5 % sequence similarity), followed by Faecalitalea cylindroides KCTC 5815T (92.2 %), Faecalicoccus acidiformans KCTC 15521T (90.2 %) and Holdemanella biformis KCTC 5969T (89.6 %).
34100482	3	0	theme	microbiota	696:705	arg1	modulation					678:687	the modulation	674:687	the modulation of gut microbiota	674:705	In this study, we aimed to investigate the effect of enzymatically synthesized α-galacto-oligosaccharides (ES-α-GOSs) on MetS and gut microbiota dysbiosis in HFD-fed mice, and to further investigate whether the attenuation of MetS is associated with the modulation of gut microbiota.
34100482	3	1	theme	synthesized	491:501	arg1	α-galacto-oligosaccharides					503:528	enzymatically synthesized α-galacto-oligosaccharides	477:528	enzymatically synthesized α-galacto-oligosaccharides (ES-α-GOSs)	477:540	In this study, we aimed to investigate the effect of enzymatically synthesized α-galacto-oligosaccharides (ES-α-GOSs) on MetS and gut microbiota dysbiosis in HFD-fed mice, and to further investigate whether the attenuation of MetS is associated with the modulation of gut microbiota.
34100482	3	1	theme	synthesized	491:501	arg1	ES-α-GOSs					531:539	ES-α-GOSs	531:539	ES-α-GOSs	531:539	In this study, we aimed to investigate the effect of enzymatically synthesized α-galacto-oligosaccharides (ES-α-GOSs) on MetS and gut microbiota dysbiosis in HFD-fed mice, and to further investigate whether the attenuation of MetS is associated with the modulation of gut microbiota.
34100482	1	2	theme	high-fat	261:268	arg1	diet					270:273	a high-fat diet	259:273	a high-fat diet (HFD)	259:279	The composition and structure of gut microbiota plays an important role in obesity induced by a high-fat diet (HFD) and related metabolic syndrome (MetS).
34100482	1	2	theme	high-fat	261:268	arg1	HFD					276:278	HFD	276:278	HFD	276:278	The composition and structure of gut microbiota plays an important role in obesity induced by a high-fat diet (HFD) and related metabolic syndrome (MetS).
34100482	3	3	theme	α-galacto-oligosaccharides	503:528	arg1	effect					467:472	the effect	463:472	the effect of enzymatically synthesized α-galacto-oligosaccharides (ES-α-GOSs) on MetS and gut microbiota dysbiosis in HFD-fed mice	463:593	In this study, we aimed to investigate the effect of enzymatically synthesized α-galacto-oligosaccharides (ES-α-GOSs) on MetS and gut microbiota dysbiosis in HFD-fed mice, and to further investigate whether the attenuation of MetS is associated with the modulation of gut microbiota.
34100482	3	4	from	MetS	545:548	arg1	mice					590:593	HFD-fed mice	582:593	HFD-fed mice	582:593	In this study, we aimed to investigate the effect of enzymatically synthesized α-galacto-oligosaccharides (ES-α-GOSs) on MetS and gut microbiota dysbiosis in HFD-fed mice, and to further investigate whether the attenuation of MetS is associated with the modulation of gut microbiota.
34100482	6	5	theme	key	1133:1135	arg1	bacteria					1137:1144	40 key bacteria	1130:1144	40 key bacteria reversed by ES-α-GOS	1130:1165	Moreover, Spearman correlation analysis showed that 40 key bacteria reversed by ES-α-GOS were highly associated with metabolic parameters.
34100482	5	6	theme	gut	1031:1033	arg1	disorder					1046:1053	the gut microbiota disorder	1027:1053	the gut microbiota disorder caused by HFD feeding	1027:1075	The subsequent analysis of gut microbiota further showed that ES-α-GOS supplements can significantly modulate the overall composition of the gut microbiota and reverse the gut microbiota disorder caused by HFD feeding.
34100482	5	7	theme	HFD	1065:1067	arg1	feeding					1069:1075	HFD feeding	1065:1075	HFD feeding	1065:1075	The subsequent analysis of gut microbiota further showed that ES-α-GOS supplements can significantly modulate the overall composition of the gut microbiota and reverse the gut microbiota disorder caused by HFD feeding.
34100482	4	8	theme	obesity-related	769:783	arg1	MetS					785:788	obesity-related MetS	769:788	obesity-related MetS	769:788	Our results indicated that ES-α-GOS could notably ameliorate obesity-related MetS, including hyperlipidemia, insulin resistance and mild inflammation.
34100482	4	8	theme	obesity-related	769:783	arg1	hyperlipidemia					801:814	hyperlipidemia	801:814	hyperlipidemia	801:814	Our results indicated that ES-α-GOS could notably ameliorate obesity-related MetS, including hyperlipidemia, insulin resistance and mild inflammation.
34100482	4	8	theme	obesity-related	769:783	arg1	resistance					825:834	insulin resistance	817:834	insulin resistance	817:834	Our results indicated that ES-α-GOS could notably ameliorate obesity-related MetS, including hyperlipidemia, insulin resistance and mild inflammation.
34100482	4	8	theme	obesity-related	769:783	arg1	inflammation					845:856	mild inflammation	840:856	mild inflammation	840:856	Our results indicated that ES-α-GOS could notably ameliorate obesity-related MetS, including hyperlipidemia, insulin resistance and mild inflammation.
34100482	3	9	theme	microbiota	558:567	arg1	dysbiosis					569:577	gut microbiota dysbiosis	554:577	gut microbiota dysbiosis	554:577	In this study, we aimed to investigate the effect of enzymatically synthesized α-galacto-oligosaccharides (ES-α-GOSs) on MetS and gut microbiota dysbiosis in HFD-fed mice, and to further investigate whether the attenuation of MetS is associated with the modulation of gut microbiota.
34100482	5	10	theme	microbiota	1004:1013	arg1	composition					981:991	the overall composition	969:991	the overall composition of the gut microbiota	969:1013	The subsequent analysis of gut microbiota further showed that ES-α-GOS supplements can significantly modulate the overall composition of the gut microbiota and reverse the gut microbiota disorder caused by HFD feeding.
34100482	5	11	theme	ES-α-GOS	921:928	arg1	supplements					930:940	ES-α-GOS supplements	921:940	ES-α-GOS supplements	921:940	The subsequent analysis of gut microbiota further showed that ES-α-GOS supplements can significantly modulate the overall composition of the gut microbiota and reverse the gut microbiota disorder caused by HFD feeding.
34100482	7	12	theme	microbiota	1371:1380	arg1	modulation					1353:1362	the modulation	1349:1362	the modulation of gut microbiota	1349:1380	These results suggested that ES-α-GOSs could serve as a potential candidate for preventing obesity-induced MetS in association with the modulation of gut microbiota.
34100482	5	13	theme	microbiota	890:899	arg1	analysis					874:881	The subsequent analysis	859:881	The subsequent analysis of gut microbiota	859:899	The subsequent analysis of gut microbiota further showed that ES-α-GOS supplements can significantly modulate the overall composition of the gut microbiota and reverse the gut microbiota disorder caused by HFD feeding.
34100482	0	14	from	syndrome	72:79	arg1	mice					106:109	high-fat diet induced mice	84:109	high-fat diet induced mice	84:109	Enzymatically synthesized α-galactooligosaccharides attenuate metabolic syndrome in high-fat diet induced mice in association with the modulation of gut microbiota.
34100482	7	15	with	association	1332:1342	arg1	modulation					1353:1362	the modulation	1349:1362	the modulation of gut microbiota	1349:1380	These results suggested that ES-α-GOSs could serve as a potential candidate for preventing obesity-induced MetS in association with the modulation of gut microbiota.
34100482	1	16	theme	gut	198:200	arg1	microbiota					202:211	gut microbiota	198:211	gut microbiota	198:211	The composition and structure of gut microbiota plays an important role in obesity induced by a high-fat diet (HFD) and related metabolic syndrome (MetS).
34100482	0	17	theme	synthesized	14:24	arg1	α-galactooligosaccharides					26:50	Enzymatically synthesized α-galactooligosaccharides	0:50	Enzymatically synthesized α-galactooligosaccharides	0:50	Enzymatically synthesized α-galactooligosaccharides attenuate metabolic syndrome in high-fat diet induced mice in association with the modulation of gut microbiota.
34100482	3	18	from	effect	467:472	arg1	MetS					545:548	MetS	545:548	MetS	545:548	In this study, we aimed to investigate the effect of enzymatically synthesized α-galacto-oligosaccharides (ES-α-GOSs) on MetS and gut microbiota dysbiosis in HFD-fed mice, and to further investigate whether the attenuation of MetS is associated with the modulation of gut microbiota.
34100482	3	18	from	effect	467:472	arg1	dysbiosis					569:577	gut microbiota dysbiosis	554:577	gut microbiota dysbiosis	554:577	In this study, we aimed to investigate the effect of enzymatically synthesized α-galacto-oligosaccharides (ES-α-GOSs) on MetS and gut microbiota dysbiosis in HFD-fed mice, and to further investigate whether the attenuation of MetS is associated with the modulation of gut microbiota.
34100482	5	19	theme	overall	973:979	arg1	composition					981:991	the overall composition	969:991	the overall composition of the gut microbiota	969:1013	The subsequent analysis of gut microbiota further showed that ES-α-GOS supplements can significantly modulate the overall composition of the gut microbiota and reverse the gut microbiota disorder caused by HFD feeding.
34100482	1	20	theme	microbiota	202:211	arg1	composition					169:179	composition	169:179	composition	169:179	The composition and structure of gut microbiota plays an important role in obesity induced by a high-fat diet (HFD) and related metabolic syndrome (MetS).
34100482	1	20	theme	microbiota	202:211	arg1	structure					185:193	structure	185:193	structure	185:193	The composition and structure of gut microbiota plays an important role in obesity induced by a high-fat diet (HFD) and related metabolic syndrome (MetS).
34100482	6	21	theme	metabolic	1195:1203	arg1	parameters					1205:1214	metabolic parameters	1195:1214	metabolic parameters	1195:1214	Moreover, Spearman correlation analysis showed that 40 key bacteria reversed by ES-α-GOS were highly associated with metabolic parameters.
34100482	6	22	theme	correlation	1097:1107	arg1	analysis					1109:1116	Spearman correlation analysis	1088:1116	Spearman correlation analysis	1088:1116	Moreover, Spearman correlation analysis showed that 40 key bacteria reversed by ES-α-GOS were highly associated with metabolic parameters.
34100482	5	23	theme	subsequent	863:872	arg1	analysis					874:881	The subsequent analysis	859:881	The subsequent analysis of gut microbiota	859:899	The subsequent analysis of gut microbiota further showed that ES-α-GOS supplements can significantly modulate the overall composition of the gut microbiota and reverse the gut microbiota disorder caused by HFD feeding.
34100482	5	24	theme	microbiota	1035:1044	arg1	disorder					1046:1053	the gut microbiota disorder	1027:1053	the gut microbiota disorder caused by HFD feeding	1027:1075	The subsequent analysis of gut microbiota further showed that ES-α-GOS supplements can significantly modulate the overall composition of the gut microbiota and reverse the gut microbiota disorder caused by HFD feeding.
34100482	0	25	theme	gut	149:151	arg1	microbiota					153:162	gut microbiota	149:162	gut microbiota	149:162	Enzymatically synthesized α-galactooligosaccharides attenuate metabolic syndrome in high-fat diet induced mice in association with the modulation of gut microbiota.
34100482	0	26	with	association	114:124	arg1	modulation					135:144	the modulation	131:144	the modulation of gut microbiota	131:162	Enzymatically synthesized α-galactooligosaccharides attenuate metabolic syndrome in high-fat diet induced mice in association with the modulation of gut microbiota.
34100482	3	27	theme	gut	554:556	arg1	dysbiosis					569:577	gut microbiota dysbiosis	554:577	gut microbiota dysbiosis	554:577	In this study, we aimed to investigate the effect of enzymatically synthesized α-galacto-oligosaccharides (ES-α-GOSs) on MetS and gut microbiota dysbiosis in HFD-fed mice, and to further investigate whether the attenuation of MetS is associated with the modulation of gut microbiota.
34100482	6	28	theme	Spearman	1088:1095	arg1	analysis					1109:1116	Spearman correlation analysis	1088:1116	Spearman correlation analysis	1088:1116	Moreover, Spearman correlation analysis showed that 40 key bacteria reversed by ES-α-GOS were highly associated with metabolic parameters.
34100482	1	29	theme	related	285:291	arg1	MetS					313:316	MetS	313:316	MetS	313:316	The composition and structure of gut microbiota plays an important role in obesity induced by a high-fat diet (HFD) and related metabolic syndrome (MetS).
34100482	1	29	theme	related	285:291	arg1	syndrome					303:310	related metabolic syndrome	285:310	related metabolic syndrome (MetS)	285:317	The composition and structure of gut microbiota plays an important role in obesity induced by a high-fat diet (HFD) and related metabolic syndrome (MetS).
34100482	1	30	dep	composition	169:179	arg1	The					165:167	The	165:167	The	165:167	The composition and structure of gut microbiota plays an important role in obesity induced by a high-fat diet (HFD) and related metabolic syndrome (MetS).
34100482	1	31	theme	important	222:230	arg1	role					232:235	an important role	219:235	an important role	219:235	The composition and structure of gut microbiota plays an important role in obesity induced by a high-fat diet (HFD) and related metabolic syndrome (MetS).
34100482	1	32	theme	metabolic	293:301	arg1	MetS					313:316	MetS	313:316	MetS	313:316	The composition and structure of gut microbiota plays an important role in obesity induced by a high-fat diet (HFD) and related metabolic syndrome (MetS).
34100482	1	32	theme	metabolic	293:301	arg1	syndrome					303:310	related metabolic syndrome	285:310	related metabolic syndrome (MetS)	285:317	The composition and structure of gut microbiota plays an important role in obesity induced by a high-fat diet (HFD) and related metabolic syndrome (MetS).
34100482	3	33	theme	MetS	650:653	arg1	attenuation					635:645	the attenuation	631:645	the attenuation of MetS	631:653	In this study, we aimed to investigate the effect of enzymatically synthesized α-galacto-oligosaccharides (ES-α-GOSs) on MetS and gut microbiota dysbiosis in HFD-fed mice, and to further investigate whether the attenuation of MetS is associated with the modulation of gut microbiota.
34100482	2	34	theme	anti-obesity	403:414	arg1	effect					416:421	an effective anti-obesity effect	390:421	an effective anti-obesity effect	390:421	Previous studies have shown that galacto-oligosaccharides (GOSs) have an effective anti-obesity effect.
34100482	0	35	theme	metabolic	62:70	arg1	syndrome					72:79	metabolic syndrome	62:79	metabolic syndrome in high-fat diet induced mice	62:109	Enzymatically synthesized α-galactooligosaccharides attenuate metabolic syndrome in high-fat diet induced mice in association with the modulation of gut microbiota.
34100482	0	36	theme	microbiota	153:162	arg1	modulation					135:144	the modulation	131:144	the modulation of gut microbiota	131:162	Enzymatically synthesized α-galactooligosaccharides attenuate metabolic syndrome in high-fat diet induced mice in association with the modulation of gut microbiota.
34100482	2	37	contain	have	385:388	arg1	GOSs					379:382	GOSs	379:382	GOSs	379:382	Previous studies have shown that galacto-oligosaccharides (GOSs) have an effective anti-obesity effect.
34100482	2	37	contain	have	385:388	arg2	effect					416:421	an effective anti-obesity effect	390:421	an effective anti-obesity effect	390:421	Previous studies have shown that galacto-oligosaccharides (GOSs) have an effective anti-obesity effect.
34100482	2	37	contain	have	385:388	arg1	galacto-oligosaccharides					353:376	galacto-oligosaccharides	353:376	galacto-oligosaccharides (GOSs)	353:383	Previous studies have shown that galacto-oligosaccharides (GOSs) have an effective anti-obesity effect.
34100482	2	38	theme	effective	393:401	arg1	effect					416:421	an effective anti-obesity effect	390:421	an effective anti-obesity effect	390:421	Previous studies have shown that galacto-oligosaccharides (GOSs) have an effective anti-obesity effect.
34100482	0	39	theme	high-fat	84:91	arg1	diet					93:96	high-fat diet	84:96	high-fat diet	84:96	Enzymatically synthesized α-galactooligosaccharides attenuate metabolic syndrome in high-fat diet induced mice in association with the modulation of gut microbiota.
34100482	3	40	theme	HFD-fed	582:588	arg1	mice					590:593	HFD-fed mice	582:593	HFD-fed mice	582:593	In this study, we aimed to investigate the effect of enzymatically synthesized α-galacto-oligosaccharides (ES-α-GOSs) on MetS and gut microbiota dysbiosis in HFD-fed mice, and to further investigate whether the attenuation of MetS is associated with the modulation of gut microbiota.
34100482	7	41	theme	potential	1273:1281	arg1	ES-α-GOSs					1246:1254	ES-α-GOSs	1246:1254	ES-α-GOSs	1246:1254	These results suggested that ES-α-GOSs could serve as a potential candidate for preventing obesity-induced MetS in association with the modulation of gut microbiota.
34100482	7	41	theme	potential	1273:1281	arg1	candidate					1283:1291	a potential candidate	1271:1291	a potential candidate for preventing obesity-induced MetS in association with the modulation of gut microbiota	1271:1380	These results suggested that ES-α-GOSs could serve as a potential candidate for preventing obesity-induced MetS in association with the modulation of gut microbiota.
34100482	4	42	theme	mild	840:843	arg1	inflammation					845:856	mild inflammation	840:856	mild inflammation	840:856	Our results indicated that ES-α-GOS could notably ameliorate obesity-related MetS, including hyperlipidemia, insulin resistance and mild inflammation.
34100482	2	43	theme	Previous	320:327	arg1	studies					329:335	Previous studies	320:335	Previous studies	320:335	Previous studies have shown that galacto-oligosaccharides (GOSs) have an effective anti-obesity effect.
34100482	0	44	dep	induced	98:104	arg1	diet					93:96	high-fat diet	84:96	high-fat diet	84:96	Enzymatically synthesized α-galactooligosaccharides attenuate metabolic syndrome in high-fat diet induced mice in association with the modulation of gut microbiota.
34100482	5	45	theme	gut	1000:1002	arg1	microbiota					1004:1013	the gut microbiota	996:1013	the gut microbiota	996:1013	The subsequent analysis of gut microbiota further showed that ES-α-GOS supplements can significantly modulate the overall composition of the gut microbiota and reverse the gut microbiota disorder caused by HFD feeding.
34100482	3	46	from	dysbiosis	569:577	arg1	mice					590:593	HFD-fed mice	582:593	HFD-fed mice	582:593	In this study, we aimed to investigate the effect of enzymatically synthesized α-galacto-oligosaccharides (ES-α-GOSs) on MetS and gut microbiota dysbiosis in HFD-fed mice, and to further investigate whether the attenuation of MetS is associated with the modulation of gut microbiota.
34100482	0	47	theme	induced	98:104	arg1	mice					106:109	high-fat diet induced mice	84:109	high-fat diet induced mice	84:109	Enzymatically synthesized α-galactooligosaccharides attenuate metabolic syndrome in high-fat diet induced mice in association with the modulation of gut microbiota.
34100482	4	48	theme	insulin	817:823	arg1	resistance					825:834	insulin resistance	817:834	insulin resistance	817:834	Our results indicated that ES-α-GOS could notably ameliorate obesity-related MetS, including hyperlipidemia, insulin resistance and mild inflammation.
34100482	7	49	theme	gut	1367:1369	arg1	microbiota					1371:1380	gut microbiota	1367:1380	gut microbiota	1367:1380	These results suggested that ES-α-GOSs could serve as a potential candidate for preventing obesity-induced MetS in association with the modulation of gut microbiota.
34100482	7	50	theme	obesity-induced	1308:1322	arg1	MetS					1324:1327	obesity-induced MetS	1308:1327	obesity-induced MetS	1308:1327	These results suggested that ES-α-GOSs could serve as a potential candidate for preventing obesity-induced MetS in association with the modulation of gut microbiota.
34100482	5	51	theme	gut	886:888	arg1	microbiota					890:899	gut microbiota	886:899	gut microbiota	886:899	The subsequent analysis of gut microbiota further showed that ES-α-GOS supplements can significantly modulate the overall composition of the gut microbiota and reverse the gut microbiota disorder caused by HFD feeding.
34100482	3	52	theme	gut	692:694	arg1	microbiota					696:705	gut microbiota	692:705	gut microbiota	692:705	In this study, we aimed to investigate the effect of enzymatically synthesized α-galacto-oligosaccharides (ES-α-GOSs) on MetS and gut microbiota dysbiosis in HFD-fed mice, and to further investigate whether the attenuation of MetS is associated with the modulation of gut microbiota.
32420550	2	0	theme	polydopamine	548:559	arg1	nanofibres					593:602	polydopamine crosslinked electrospun gelatin nanofibres	548:602	polydopamine crosslinked electrospun gelatin nanofibres containing mineralized magnesium	548:635	To achieve this goal, we investigated the effect of chondroitin sulphate on the morphology, mechanical properties, wettability and biocompatibility of polydopamine crosslinked electrospun gelatin nanofibres containing mineralized magnesium.
32420550	6	1	theme	biopsied	1160:1167	arg1	tissues					1169:1175	the biopsied tissues	1156:1175	the biopsied tissues	1156:1175	Histology of the biopsied tissues indicated smooth regeneration and collagen organization of the burns treated with core-shell nanostructures than untreated burns.
32420550	7	2	theme	burn	1431:1434	arg1	healing					1442:1448	burn wound healing	1431:1448	burn wound healing	1431:1448	This study compared the physico-chemical and biological properties of composite nanofibres that are capable of accelerating burn wound healing and possess antimicrobial properties, highlighting their potential as wound dressings and skin substitutes.
32420550	0	3	theme	chondroitin	95:105	arg1	sulphate					107:114	chondroitin sulphate	95:114	chondroitin sulphate	95:114	Wound healing properties of magnesium mineralized antimicrobial nanofibre dressings containing chondroitin sulphate - a comparison between blend and core-shell nanofibres.
32420550	6	4	theme	core-shell	1259:1268	arg1	nanostructures					1270:1283	core-shell nanostructures	1259:1283	core-shell nanostructures than untreated burns	1259:1304	Histology of the biopsied tissues indicated smooth regeneration and collagen organization of the burns treated with core-shell nanostructures than untreated burns.
32420550	4	5	theme	Nanofibre	790:798	arg1	blends					800:805	Nanofibre blends	790:805	Nanofibre blends	790:805	Nanofibre blends presented greater tensile strength and stretchability, while core-shell nanofibres displayed superior photoluminescent properties.
32420550	3	6	dep	blend	758:762	arg1	nanofibres					778:787	nanofibres	778:787	nanofibres	778:787	To extend the durability of dressings, we prepared composite dressings containing polycaprolactone (PCL) and gelatin as blend or core-shell nanofibres.
32420550	2	7	theme	mineralized	615:625	arg1	magnesium					627:635	mineralized magnesium	615:635	mineralized magnesium	615:635	To achieve this goal, we investigated the effect of chondroitin sulphate on the morphology, mechanical properties, wettability and biocompatibility of polydopamine crosslinked electrospun gelatin nanofibres containing mineralized magnesium.
32420550	7	8	theme	antimicrobial	1462:1474	arg1	properties					1476:1485	antimicrobial properties	1462:1485	antimicrobial properties	1462:1485	This study compared the physico-chemical and biological properties of composite nanofibres that are capable of accelerating burn wound healing and possess antimicrobial properties, highlighting their potential as wound dressings and skin substitutes.
32420550	1	9	theme	tissue	303:308	arg1	regeneration					310:321	tissue regeneration	303:321	tissue regeneration	303:321	The development of antimicrobial nanofibre dressings that can protect the injured tissues from commensal pathogens while promoting tissue regeneration finds enormous potential in plastic and reconstructive surgery practices.
32420550	7	10	theme	composite	1377:1385	arg1	nanofibres					1387:1396	composite nanofibres	1377:1396	composite nanofibres that are capable of accelerating burn wound healing and possess antimicrobial properties	1377:1485	This study compared the physico-chemical and biological properties of composite nanofibres that are capable of accelerating burn wound healing and possess antimicrobial properties, highlighting their potential as wound dressings and skin substitutes.
32420550	5	11	theme	porcine	943:949	arg1	model					951:955	a porcine model	941:955	a porcine model of cutaneous burn injury	941:980	In a porcine model of cutaneous burn injury, both the blend and core-shell nanofibre dressings displayed improved re-epithelialization, wound closure and clinical outcome in comparison to untreated burns.
32420550	4	12	theme	core-shell	868:877	arg1	nanofibres					879:888	core-shell nanofibres	868:888	core-shell nanofibres	868:888	Nanofibre blends presented greater tensile strength and stretchability, while core-shell nanofibres displayed superior photoluminescent properties.
32420550	5	13	theme	wound	1074:1078	arg1	closure					1080:1086	wound closure	1074:1086	wound closure	1074:1086	In a porcine model of cutaneous burn injury, both the blend and core-shell nanofibre dressings displayed improved re-epithelialization, wound closure and clinical outcome in comparison to untreated burns.
32420550	2	14	theme	nanofibres	593:602	arg1	wettability					512:522	wettability	512:522	wettability	512:522	To achieve this goal, we investigated the effect of chondroitin sulphate on the morphology, mechanical properties, wettability and biocompatibility of polydopamine crosslinked electrospun gelatin nanofibres containing mineralized magnesium.
32420550	2	14	theme	nanofibres	593:602	arg1	morphology					477:486	morphology	477:486	morphology	477:486	To achieve this goal, we investigated the effect of chondroitin sulphate on the morphology, mechanical properties, wettability and biocompatibility of polydopamine crosslinked electrospun gelatin nanofibres containing mineralized magnesium.
32420550	2	14	theme	nanofibres	593:602	arg1	properties					500:509	mechanical properties	489:509	mechanical properties	489:509	To achieve this goal, we investigated the effect of chondroitin sulphate on the morphology, mechanical properties, wettability and biocompatibility of polydopamine crosslinked electrospun gelatin nanofibres containing mineralized magnesium.
32420550	2	14	theme	nanofibres	593:602	arg1	biocompatibility					528:543	biocompatibility	528:543	biocompatibility	528:543	To achieve this goal, we investigated the effect of chondroitin sulphate on the morphology, mechanical properties, wettability and biocompatibility of polydopamine crosslinked electrospun gelatin nanofibres containing mineralized magnesium.
32420550	5	15	theme	improved	1043:1050	arg1	re-epithelialization					1052:1071	improved re-epithelialization	1043:1071	improved re-epithelialization	1043:1071	In a porcine model of cutaneous burn injury, both the blend and core-shell nanofibre dressings displayed improved re-epithelialization, wound closure and clinical outcome in comparison to untreated burns.
32420550	6	16	theme	burns	1240:1244	arg1	regeneration					1194:1205	smooth regeneration	1187:1205	smooth regeneration	1187:1205	Histology of the biopsied tissues indicated smooth regeneration and collagen organization of the burns treated with core-shell nanostructures than untreated burns.
32420550	6	16	theme	burns	1240:1244	arg1	organization					1220:1231	collagen organization	1211:1231	collagen organization	1211:1231	Histology of the biopsied tissues indicated smooth regeneration and collagen organization of the burns treated with core-shell nanostructures than untreated burns.
32420550	4	17	theme	superior	900:907	arg1	properties					926:935	superior photoluminescent properties	900:935	superior photoluminescent properties	900:935	Nanofibre blends presented greater tensile strength and stretchability, while core-shell nanofibres displayed superior photoluminescent properties.
32420550	5	18	theme	blend	992:996	arg1	dressings					1023:1031	both the blend and core-shell nanofibre dressings	983:1031	dressings	1023:1031	In a porcine model of cutaneous burn injury, both the blend and core-shell nanofibre dressings displayed improved re-epithelialization, wound closure and clinical outcome in comparison to untreated burns.
32420550	1	19	theme	antimicrobial	191:203	arg1	dressings					215:223	antimicrobial nanofibre dressings	191:223	antimicrobial nanofibre dressings that can protect the injured tissues from commensal pathogens while promoting tissue regeneration	191:321	The development of antimicrobial nanofibre dressings that can protect the injured tissues from commensal pathogens while promoting tissue regeneration finds enormous potential in plastic and reconstructive surgery practices.
32420550	2	20	theme	gelatin	585:591	arg1	nanofibres					593:602	polydopamine crosslinked electrospun gelatin nanofibres	548:602	polydopamine crosslinked electrospun gelatin nanofibres containing mineralized magnesium	548:635	To achieve this goal, we investigated the effect of chondroitin sulphate on the morphology, mechanical properties, wettability and biocompatibility of polydopamine crosslinked electrospun gelatin nanofibres containing mineralized magnesium.
32420550	2	21	dep	morphology	477:486	arg1	the					473:475	the	473:475	the	473:475	To achieve this goal, we investigated the effect of chondroitin sulphate on the morphology, mechanical properties, wettability and biocompatibility of polydopamine crosslinked electrospun gelatin nanofibres containing mineralized magnesium.
32420550	2	22	theme	mechanical	489:498	arg1	properties					500:509	mechanical properties	489:509	mechanical properties	489:509	To achieve this goal, we investigated the effect of chondroitin sulphate on the morphology, mechanical properties, wettability and biocompatibility of polydopamine crosslinked electrospun gelatin nanofibres containing mineralized magnesium.
32420550	1	23	theme	nanofibre	205:213	arg1	dressings					215:223	antimicrobial nanofibre dressings	191:223	antimicrobial nanofibre dressings that can protect the injured tissues from commensal pathogens while promoting tissue regeneration	191:321	The development of antimicrobial nanofibre dressings that can protect the injured tissues from commensal pathogens while promoting tissue regeneration finds enormous potential in plastic and reconstructive surgery practices.
32420550	0	24	theme	healing	6:12	arg1	properties					14:23	Wound healing properties	0:23	Wound healing properties of magnesium mineralized antimicrobial nanofibre dressings	0:82	Wound healing properties of magnesium mineralized antimicrobial nanofibre dressings containing chondroitin sulphate - a comparison between blend and core-shell nanofibres.
32420550	7	25	contain	possess	1454:1460	arg1	nanofibres					1387:1396	composite nanofibres	1377:1396	composite nanofibres that are capable of accelerating burn wound healing and possess antimicrobial properties	1377:1485	This study compared the physico-chemical and biological properties of composite nanofibres that are capable of accelerating burn wound healing and possess antimicrobial properties, highlighting their potential as wound dressings and skin substitutes.
32420550	7	25	contain	possess	1454:1460	arg2	properties					1476:1485	antimicrobial properties	1462:1485	antimicrobial properties	1462:1485	This study compared the physico-chemical and biological properties of composite nanofibres that are capable of accelerating burn wound healing and possess antimicrobial properties, highlighting their potential as wound dressings and skin substitutes.
32420550	1	26	theme	dressings	215:223	arg1	development					176:186	The development	172:186	The development of antimicrobial nanofibre dressings that can protect the injured tissues from commensal pathogens while promoting tissue regeneration	172:321	The development of antimicrobial nanofibre dressings that can protect the injured tissues from commensal pathogens while promoting tissue regeneration finds enormous potential in plastic and reconstructive surgery practices.
32420550	0	27	theme	Wound	0:4	arg1	healing					6:12	Wound healing	0:12	Wound healing properties of magnesium mineralized antimicrobial nanofibre dressings	0:82	Wound healing properties of magnesium mineralized antimicrobial nanofibre dressings containing chondroitin sulphate - a comparison between blend and core-shell nanofibres.
32420550	3	28	theme	dressings	666:674	arg1	durability					652:661	the durability	648:661	the durability of dressings	648:674	To extend the durability of dressings, we prepared composite dressings containing polycaprolactone (PCL) and gelatin as blend or core-shell nanofibres.
32420550	5	29	theme	untreated	1126:1134	arg1	burns					1136:1140	untreated burns	1126:1140	untreated burns	1126:1140	In a porcine model of cutaneous burn injury, both the blend and core-shell nanofibre dressings displayed improved re-epithelialization, wound closure and clinical outcome in comparison to untreated burns.
32420550	7	30	theme	physico-chemical	1331:1346	arg1	properties					1363:1372	the physico-chemical and biological properties	1327:1372	the physico-chemical and biological properties of composite nanofibres that are capable of accelerating burn wound healing and possess antimicrobial properties	1327:1485	This study compared the physico-chemical and biological properties of composite nanofibres that are capable of accelerating burn wound healing and possess antimicrobial properties, highlighting their potential as wound dressings and skin substitutes.
32420550	1	31	theme	enormous	329:336	arg1	potential					338:346	enormous potential	329:346	enormous potential	329:346	The development of antimicrobial nanofibre dressings that can protect the injured tissues from commensal pathogens while promoting tissue regeneration finds enormous potential in plastic and reconstructive surgery practices.
32420550	5	32	theme	clinical	1092:1099	arg1	outcome					1101:1107	clinical outcome	1092:1107	clinical outcome	1092:1107	In a porcine model of cutaneous burn injury, both the blend and core-shell nanofibre dressings displayed improved re-epithelialization, wound closure and clinical outcome in comparison to untreated burns.
32420550	5	33	theme	cutaneous	960:968	arg1	injury					975:980	cutaneous burn injury	960:980	cutaneous burn injury	960:980	In a porcine model of cutaneous burn injury, both the blend and core-shell nanofibre dressings displayed improved re-epithelialization, wound closure and clinical outcome in comparison to untreated burns.
32420550	7	34	theme	wound	1436:1440	arg1	healing					1442:1448	burn wound healing	1431:1448	burn wound healing	1431:1448	This study compared the physico-chemical and biological properties of composite nanofibres that are capable of accelerating burn wound healing and possess antimicrobial properties, highlighting their potential as wound dressings and skin substitutes.
32420550	5	35	theme	burn	970:973	arg1	injury					975:980	cutaneous burn injury	960:980	cutaneous burn injury	960:980	In a porcine model of cutaneous burn injury, both the blend and core-shell nanofibre dressings displayed improved re-epithelialization, wound closure and clinical outcome in comparison to untreated burns.
32420550	0	36	theme	mineralized	38:48	arg1	dressings					74:82	magnesium mineralized antimicrobial nanofibre dressings	28:82	magnesium mineralized antimicrobial nanofibre dressings	28:82	Wound healing properties of magnesium mineralized antimicrobial nanofibre dressings containing chondroitin sulphate - a comparison between blend and core-shell nanofibres.
32420550	5	37	theme	injury	975:980	arg1	model					951:955	a porcine model	941:955	a porcine model of cutaneous burn injury	941:980	In a porcine model of cutaneous burn injury, both the blend and core-shell nanofibre dressings displayed improved re-epithelialization, wound closure and clinical outcome in comparison to untreated burns.
32420550	2	38	theme	sulphate	461:468	arg1	effect					439:444	the effect	435:444	the effect of chondroitin sulphate on the morphology, mechanical properties, wettability and biocompatibility of polydopamine crosslinked electrospun gelatin nanofibres containing mineralized magnesium	435:635	To achieve this goal, we investigated the effect of chondroitin sulphate on the morphology, mechanical properties, wettability and biocompatibility of polydopamine crosslinked electrospun gelatin nanofibres containing mineralized magnesium.
32420550	0	39	theme	magnesium	28:36	arg1	dressings					74:82	magnesium mineralized antimicrobial nanofibre dressings	28:82	magnesium mineralized antimicrobial nanofibre dressings	28:82	Wound healing properties of magnesium mineralized antimicrobial nanofibre dressings containing chondroitin sulphate - a comparison between blend and core-shell nanofibres.
32420550	1	40	theme	plastic	351:357	arg1	practices					386:394	plastic and reconstructive surgery practices	351:394	plastic and reconstructive surgery practices	351:394	The development of antimicrobial nanofibre dressings that can protect the injured tissues from commensal pathogens while promoting tissue regeneration finds enormous potential in plastic and reconstructive surgery practices.
32420550	0	41	theme	core-shell	149:158	arg1	nanofibres					160:169	core-shell nanofibres	149:169	core-shell nanofibres	149:169	Wound healing properties of magnesium mineralized antimicrobial nanofibre dressings containing chondroitin sulphate - a comparison between blend and core-shell nanofibres.
32420550	3	42	theme	composite	689:697	arg1	dressings					699:707	composite dressings	689:707	composite dressings containing polycaprolactone (PCL) and gelatin as blend or core-shell nanofibres	689:787	To extend the durability of dressings, we prepared composite dressings containing polycaprolactone (PCL) and gelatin as blend or core-shell nanofibres.
32420550	1	43	theme	injured	246:252	arg1	tissues					254:260	the injured tissues	242:260	the injured tissues from commensal pathogens	242:285	The development of antimicrobial nanofibre dressings that can protect the injured tissues from commensal pathogens while promoting tissue regeneration finds enormous potential in plastic and reconstructive surgery practices.
32420550	0	44	theme	nanofibre	64:72	arg1	dressings					74:82	magnesium mineralized antimicrobial nanofibre dressings	28:82	magnesium mineralized antimicrobial nanofibre dressings	28:82	Wound healing properties of magnesium mineralized antimicrobial nanofibre dressings containing chondroitin sulphate - a comparison between blend and core-shell nanofibres.
32420550	7	45	theme	nanofibres	1387:1396	arg1	properties					1363:1372	the physico-chemical and biological properties	1327:1372	the physico-chemical and biological properties of composite nanofibres that are capable of accelerating burn wound healing and possess antimicrobial properties	1327:1485	This study compared the physico-chemical and biological properties of composite nanofibres that are capable of accelerating burn wound healing and possess antimicrobial properties, highlighting their potential as wound dressings and skin substitutes.
32420550	6	46	theme	smooth	1187:1192	arg1	regeneration					1194:1205	smooth regeneration	1187:1205	smooth regeneration	1187:1205	Histology of the biopsied tissues indicated smooth regeneration and collagen organization of the burns treated with core-shell nanostructures than untreated burns.
32420550	2	47	theme	crosslinked	561:571	arg1	nanofibres					593:602	polydopamine crosslinked electrospun gelatin nanofibres	548:602	polydopamine crosslinked electrospun gelatin nanofibres containing mineralized magnesium	548:635	To achieve this goal, we investigated the effect of chondroitin sulphate on the morphology, mechanical properties, wettability and biocompatibility of polydopamine crosslinked electrospun gelatin nanofibres containing mineralized magnesium.
32420550	4	48	theme	greater	817:823	arg1	strength					833:840	greater tensile strength	817:840	greater tensile strength	817:840	Nanofibre blends presented greater tensile strength and stretchability, while core-shell nanofibres displayed superior photoluminescent properties.
32420550	0	49	theme	antimicrobial	50:62	arg1	dressings					74:82	magnesium mineralized antimicrobial nanofibre dressings	28:82	magnesium mineralized antimicrobial nanofibre dressings	28:82	Wound healing properties of magnesium mineralized antimicrobial nanofibre dressings containing chondroitin sulphate - a comparison between blend and core-shell nanofibres.
32420550	1	50	theme	reconstructive	363:376	arg1	surgery					378:384	reconstructive surgery	363:384	reconstructive surgery	363:384	The development of antimicrobial nanofibre dressings that can protect the injured tissues from commensal pathogens while promoting tissue regeneration finds enormous potential in plastic and reconstructive surgery practices.
32420550	1	51	from	pathogens	277:285	arg1	tissues					254:260	the injured tissues	242:260	the injured tissues from commensal pathogens	242:285	The development of antimicrobial nanofibre dressings that can protect the injured tissues from commensal pathogens while promoting tissue regeneration finds enormous potential in plastic and reconstructive surgery practices.
32420550	7	52	dep	skin	1540:1543	arg1	substitutes					1545:1555	substitutes	1545:1555	substitutes	1545:1555	This study compared the physico-chemical and biological properties of composite nanofibres that are capable of accelerating burn wound healing and possess antimicrobial properties, highlighting their potential as wound dressings and skin substitutes.
32420550	1	53	theme	surgery	378:384	arg1	practices					386:394	plastic and reconstructive surgery practices	351:394	plastic and reconstructive surgery practices	351:394	The development of antimicrobial nanofibre dressings that can protect the injured tissues from commensal pathogens while promoting tissue regeneration finds enormous potential in plastic and reconstructive surgery practices.
32420550	7	54	theme	biological	1352:1361	arg1	properties					1363:1372	the physico-chemical and biological properties	1327:1372	the physico-chemical and biological properties of composite nanofibres that are capable of accelerating burn wound healing and possess antimicrobial properties	1327:1485	This study compared the physico-chemical and biological properties of composite nanofibres that are capable of accelerating burn wound healing and possess antimicrobial properties, highlighting their potential as wound dressings and skin substitutes.
32420550	2	55	link	crosslinked	561:571	arg1	nanofibres					593:602	polydopamine crosslinked electrospun gelatin nanofibres	548:602	polydopamine crosslinked electrospun gelatin nanofibres containing mineralized magnesium	548:635	To achieve this goal, we investigated the effect of chondroitin sulphate on the morphology, mechanical properties, wettability and biocompatibility of polydopamine crosslinked electrospun gelatin nanofibres containing mineralized magnesium.
32420550	6	56	theme	untreated	1290:1298	arg1	burns					1300:1304	untreated burns	1290:1304	untreated burns	1290:1304	Histology of the biopsied tissues indicated smooth regeneration and collagen organization of the burns treated with core-shell nanostructures than untreated burns.
32420550	3	57	contain	containing	709:718	arg2	PCL					738:740	PCL	738:740	PCL	738:740	To extend the durability of dressings, we prepared composite dressings containing polycaprolactone (PCL) and gelatin as blend or core-shell nanofibres.
32420550	3	57	contain	containing	709:718	arg2	polycaprolactone					720:735	polycaprolactone	720:735	polycaprolactone (PCL)	720:741	To extend the durability of dressings, we prepared composite dressings containing polycaprolactone (PCL) and gelatin as blend or core-shell nanofibres.
32420550	3	57	contain	containing	709:718	arg2	gelatin					747:753	gelatin	747:753	gelatin	747:753	To extend the durability of dressings, we prepared composite dressings containing polycaprolactone (PCL) and gelatin as blend or core-shell nanofibres.
32420550	3	57	contain	containing	709:718	arg1	dressings					699:707	composite dressings	689:707	composite dressings containing polycaprolactone (PCL) and gelatin as blend or core-shell nanofibres	689:787	To extend the durability of dressings, we prepared composite dressings containing polycaprolactone (PCL) and gelatin as blend or core-shell nanofibres.
32420550	2	58	from	effect	439:444	arg1	wettability					512:522	wettability	512:522	wettability	512:522	To achieve this goal, we investigated the effect of chondroitin sulphate on the morphology, mechanical properties, wettability and biocompatibility of polydopamine crosslinked electrospun gelatin nanofibres containing mineralized magnesium.
32420550	2	58	from	effect	439:444	arg1	morphology					477:486	morphology	477:486	morphology	477:486	To achieve this goal, we investigated the effect of chondroitin sulphate on the morphology, mechanical properties, wettability and biocompatibility of polydopamine crosslinked electrospun gelatin nanofibres containing mineralized magnesium.
32420550	2	58	from	effect	439:444	arg1	properties					500:509	mechanical properties	489:509	mechanical properties	489:509	To achieve this goal, we investigated the effect of chondroitin sulphate on the morphology, mechanical properties, wettability and biocompatibility of polydopamine crosslinked electrospun gelatin nanofibres containing mineralized magnesium.
32420550	2	58	from	effect	439:444	arg1	biocompatibility					528:543	biocompatibility	528:543	biocompatibility	528:543	To achieve this goal, we investigated the effect of chondroitin sulphate on the morphology, mechanical properties, wettability and biocompatibility of polydopamine crosslinked electrospun gelatin nanofibres containing mineralized magnesium.
32420550	4	59	theme	photoluminescent	909:924	arg1	properties					926:935	superior photoluminescent properties	900:935	superior photoluminescent properties	900:935	Nanofibre blends presented greater tensile strength and stretchability, while core-shell nanofibres displayed superior photoluminescent properties.
32420550	4	60	theme	tensile	825:831	arg1	strength					833:840	greater tensile strength	817:840	greater tensile strength	817:840	Nanofibre blends presented greater tensile strength and stretchability, while core-shell nanofibres displayed superior photoluminescent properties.
32420550	7	61	theme	wound	1520:1524	arg1	dressings					1526:1534	wound dressings	1520:1534	wound dressings	1520:1534	This study compared the physico-chemical and biological properties of composite nanofibres that are capable of accelerating burn wound healing and possess antimicrobial properties, highlighting their potential as wound dressings and skin substitutes.
32420550	6	62	theme	tissues	1169:1175	arg1	Histology					1143:1151	Histology	1143:1151	Histology of the biopsied tissues	1143:1175	Histology of the biopsied tissues indicated smooth regeneration and collagen organization of the burns treated with core-shell nanostructures than untreated burns.
32420550	2	63	theme	electrospun	573:583	arg1	nanofibres					593:602	polydopamine crosslinked electrospun gelatin nanofibres	548:602	polydopamine crosslinked electrospun gelatin nanofibres containing mineralized magnesium	548:635	To achieve this goal, we investigated the effect of chondroitin sulphate on the morphology, mechanical properties, wettability and biocompatibility of polydopamine crosslinked electrospun gelatin nanofibres containing mineralized magnesium.
32420550	2	64	theme	chondroitin	449:459	arg1	sulphate					461:468	chondroitin sulphate	449:468	chondroitin sulphate	449:468	To achieve this goal, we investigated the effect of chondroitin sulphate on the morphology, mechanical properties, wettability and biocompatibility of polydopamine crosslinked electrospun gelatin nanofibres containing mineralized magnesium.
32420550	6	65	theme	collagen	1211:1218	arg1	organization					1220:1231	collagen organization	1211:1231	collagen organization	1211:1231	Histology of the biopsied tissues indicated smooth regeneration and collagen organization of the burns treated with core-shell nanostructures than untreated burns.
32420550	5	66	theme	core-shell	1002:1011	arg1	nanofibre					1013:1021	core-shell nanofibre	1002:1021	core-shell nanofibre	1002:1021	In a porcine model of cutaneous burn injury, both the blend and core-shell nanofibre dressings displayed improved re-epithelialization, wound closure and clinical outcome in comparison to untreated burns.
32420550	1	67	theme	commensal	267:275	arg1	pathogens					277:285	commensal pathogens	267:285	commensal pathogens	267:285	The development of antimicrobial nanofibre dressings that can protect the injured tissues from commensal pathogens while promoting tissue regeneration finds enormous potential in plastic and reconstructive surgery practices.
32420550	0	68	theme	dressings	74:82	arg1	properties					14:23	Wound healing properties	0:23	Wound healing properties of magnesium mineralized antimicrobial nanofibre dressings	0:82	Wound healing properties of magnesium mineralized antimicrobial nanofibre dressings containing chondroitin sulphate - a comparison between blend and core-shell nanofibres.
32420550	2	69	contain	containing	604:613	arg1	nanofibres					593:602	polydopamine crosslinked electrospun gelatin nanofibres	548:602	polydopamine crosslinked electrospun gelatin nanofibres containing mineralized magnesium	548:635	To achieve this goal, we investigated the effect of chondroitin sulphate on the morphology, mechanical properties, wettability and biocompatibility of polydopamine crosslinked electrospun gelatin nanofibres containing mineralized magnesium.
32420550	2	69	contain	containing	604:613	arg2	magnesium					627:635	mineralized magnesium	615:635	mineralized magnesium	615:635	To achieve this goal, we investigated the effect of chondroitin sulphate on the morphology, mechanical properties, wettability and biocompatibility of polydopamine crosslinked electrospun gelatin nanofibres containing mineralized magnesium.
32420550	5	70	theme	nanofibre	1013:1021	arg1	dressings					1023:1031	both the blend and core-shell nanofibre dressings	983:1031	dressings	1023:1031	In a porcine model of cutaneous burn injury, both the blend and core-shell nanofibre dressings displayed improved re-epithelialization, wound closure and clinical outcome in comparison to untreated burns.
33052806	5	0	theme	kashiwanohense	577:590	arg1	neighbour					628:636	the closest neighbour	616:636	the closest neighbour to strain	616:646	kashiwanohense DSM 21854T (95.4 %) was the closest neighbour to strain in Cluster I (BRDM 6T), whereas the type strain of Bifidobacterium dentium DSM 20436T (values were in the range of 98‒99.8 %) was the closest neighbour to the other six strains in Cluster II.
33052806	5	0	theme	kashiwanohense	577:590	arg1	21854T					596:601	kashiwanohense DSM 21854T	577:601	kashiwanohense DSM 21854T (95.4 %)	577:610	kashiwanohense DSM 21854T (95.4 %) was the closest neighbour to strain in Cluster I (BRDM 6T), whereas the type strain of Bifidobacterium dentium DSM 20436T (values were in the range of 98‒99.8 %) was the closest neighbour to the other six strains in Cluster II.
33052806	5	0	theme	kashiwanohense	577:590	arg1	%					609:609	95.4 %	604:609	95.4 %	604:609	kashiwanohense DSM 21854T (95.4 %) was the closest neighbour to strain in Cluster I (BRDM 6T), whereas the type strain of Bifidobacterium dentium DSM 20436T (values were in the range of 98‒99.8 %) was the closest neighbour to the other six strains in Cluster II.
33052806	6	1	from	values	878:883	arg1	Cluster					914:920	Cluster II	914:923	Cluster II	914:923	The average nucleotide identity (ANI) values of BRDM 6T and of strains in Cluster II with the closely related type strains were 76.0 and 98.9 % (mean value) respectively.
33052806	5	2	theme	%	771:771	arg1	range					754:758	the range	750:758	the range of 98‒99.8 %	750:771	kashiwanohense DSM 21854T (95.4 %) was the closest neighbour to strain in Cluster I (BRDM 6T), whereas the type strain of Bifidobacterium dentium DSM 20436T (values were in the range of 98‒99.8 %) was the closest neighbour to the other six strains in Cluster II.
33052806	7	3	theme	BRDM	1151:1154	arg1	6T					1156:1157	the strain BRDM 6T	1140:1157	the strain BRDM 6T	1140:1157	Therefore, genotyping based on the genome sequence of the strain BRDM 6T combined with phenotypic analyses clearly revealed that the strain BRDM 6T represents a novel species for which the names Bifidobacterium choloepi sp.
33052806	4	4	theme	sequence	502:509	arg1	similarity					511:520	16S rRNA gene sequence similarity	488:520	16S rRNA gene sequence similarity	488:520	On the basis of 16S rRNA gene sequence similarity, the type strain of Bifidobacterium catenulatum subsp.
33052806	5	5	theme	closest	620:626	arg1	21854T					596:601	kashiwanohense DSM 21854T	577:601	kashiwanohense DSM 21854T (95.4 %)	577:610	kashiwanohense DSM 21854T (95.4 %) was the closest neighbour to strain in Cluster I (BRDM 6T), whereas the type strain of Bifidobacterium dentium DSM 20436T (values were in the range of 98‒99.8 %) was the closest neighbour to the other six strains in Cluster II.
33052806	5	5	theme	closest	620:626	arg1	neighbour					628:636	the closest neighbour	616:636	the closest neighbour to strain	616:646	kashiwanohense DSM 21854T (95.4 %) was the closest neighbour to strain in Cluster I (BRDM 6T), whereas the type strain of Bifidobacterium dentium DSM 20436T (values were in the range of 98‒99.8 %) was the closest neighbour to the other six strains in Cluster II.
33052806	6	6	theme	6T	893:894	arg1	values					878:883	The average nucleotide identity (ANI) values	840:883	The average nucleotide identity (ANI) values of BRDM 6T and of strains in Cluster II with the closely related type strains	840:961	The average nucleotide identity (ANI) values of BRDM 6T and of strains in Cluster II with the closely related type strains were 76.0 and 98.9 % (mean value) respectively.
33052806	6	6	theme	6T	893:894	arg1	%					982:982	76.0 and 98.9 %	968:982	76.0 and 98.9 % (mean value)	968:995	The average nucleotide identity (ANI) values of BRDM 6T and of strains in Cluster II with the closely related type strains were 76.0 and 98.9 % (mean value) respectively.
33052806	5	7	theme	DSM	592:594	arg1	neighbour					628:636	the closest neighbour	616:636	the closest neighbour to strain	616:646	kashiwanohense DSM 21854T (95.4 %) was the closest neighbour to strain in Cluster I (BRDM 6T), whereas the type strain of Bifidobacterium dentium DSM 20436T (values were in the range of 98‒99.8 %) was the closest neighbour to the other six strains in Cluster II.
33052806	5	7	theme	DSM	592:594	arg1	21854T					596:601	kashiwanohense DSM 21854T	577:601	kashiwanohense DSM 21854T (95.4 %)	577:610	kashiwanohense DSM 21854T (95.4 %) was the closest neighbour to strain in Cluster I (BRDM 6T), whereas the type strain of Bifidobacterium dentium DSM 20436T (values were in the range of 98‒99.8 %) was the closest neighbour to the other six strains in Cluster II.
33052806	5	7	theme	DSM	592:594	arg1	%					609:609	95.4 %	604:609	95.4 %	604:609	kashiwanohense DSM 21854T (95.4 %) was the closest neighbour to strain in Cluster I (BRDM 6T), whereas the type strain of Bifidobacterium dentium DSM 20436T (values were in the range of 98‒99.8 %) was the closest neighbour to the other six strains in Cluster II.
33052806	6	8	with	strains	903:909	arg1	strains					955:961	the closely related type strains	930:961	the closely related type strains	930:961	The average nucleotide identity (ANI) values of BRDM 6T and of strains in Cluster II with the closely related type strains were 76.0 and 98.9 % (mean value) respectively.
33052806	4	9	theme	gene	497:500	arg1	similarity					511:520	16S rRNA gene sequence similarity	488:520	16S rRNA gene sequence similarity	488:520	On the basis of 16S rRNA gene sequence similarity, the type strain of Bifidobacterium catenulatum subsp.
33052806	0	10	theme	novel	95:99	arg1	sp					132:133	the novel taxon Bifidobacterium choloepi sp	91:133	the novel taxon Bifidobacterium choloepi sp	91:133	Bifidobacteria in two-toed sloths (Choloepus didactylus): phylogenetic characterization of the novel taxon Bifidobacterium choloepi sp.
33052806	0	11	from	Bifidobacteria	0:13	arg1	sloths					27:32	two-toed sloths	18:32	two-toed sloths (Choloepus didactylus)	18:55	Bifidobacteria in two-toed sloths (Choloepus didactylus): phylogenetic characterization of the novel taxon Bifidobacterium choloepi sp.
33052806	7	12	theme	phenotypic	1098:1107	arg1	analyses					1109:1116	phenotypic analyses	1098:1116	phenotypic analyses	1098:1116	Therefore, genotyping based on the genome sequence of the strain BRDM 6T combined with phenotypic analyses clearly revealed that the strain BRDM 6T represents a novel species for which the names Bifidobacterium choloepi sp.
33052806	6	13	from	Cluster	914:920	arg1	values					878:883	The average nucleotide identity (ANI) values	840:883	The average nucleotide identity (ANI) values of BRDM 6T and of strains in Cluster II with the closely related type strains	840:961	The average nucleotide identity (ANI) values of BRDM 6T and of strains in Cluster II with the closely related type strains were 76.0 and 98.9 % (mean value) respectively.
33052806	6	13	from	Cluster	914:920	arg1	%					982:982	76.0 and 98.9 %	968:982	76.0 and 98.9 % (mean value)	968:995	The average nucleotide identity (ANI) values of BRDM 6T and of strains in Cluster II with the closely related type strains were 76.0 and 98.9 % (mean value) respectively.
33052806	3	14	theme	rRNA	341:344	arg1	analysis					325:332	Comparative sequence analysis	304:332	Comparative sequence analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dnaG) genes	304:407	Comparative sequence analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dnaG) genes revealed that these strains were classified into two clusters.
33052806	6	15	theme	mean	985:988	arg1	%					982:982	76.0 and 98.9 %	968:982	76.0 and 98.9 % (mean value)	968:995	The average nucleotide identity (ANI) values of BRDM 6T and of strains in Cluster II with the closely related type strains were 76.0 and 98.9 % (mean value) respectively.
33052806	6	15	theme	mean	985:988	arg1	value					990:994	mean value	985:994	mean value	985:994	The average nucleotide identity (ANI) values of BRDM 6T and of strains in Cluster II with the closely related type strains were 76.0 and 98.9 % (mean value) respectively.
33052806	6	16	theme	identity	863:870	arg1	values					878:883	The average nucleotide identity (ANI) values	840:883	The average nucleotide identity (ANI) values of BRDM 6T and of strains in Cluster II with the closely related type strains	840:961	The average nucleotide identity (ANI) values of BRDM 6T and of strains in Cluster II with the closely related type strains were 76.0 and 98.9 % (mean value) respectively.
33052806	6	16	theme	identity	863:870	arg1	%					982:982	76.0 and 98.9 %	968:982	76.0 and 98.9 % (mean value)	968:995	The average nucleotide identity (ANI) values of BRDM 6T and of strains in Cluster II with the closely related type strains were 76.0 and 98.9 % (mean value) respectively.
33052806	0	17	theme	Bifidobacterium	107:121	arg1	sp					132:133	the novel taxon Bifidobacterium choloepi sp	91:133	the novel taxon Bifidobacterium choloepi sp	91:133	Bifidobacteria in two-toed sloths (Choloepus didactylus): phylogenetic characterization of the novel taxon Bifidobacterium choloepi sp.
33052806	2	18	theme	bifidobacterial	147:161	arg1	strains					163:169	Seven bifidobacterial strains	141:169	Seven bifidobacterial strains	141:169	Seven bifidobacterial strains were isolated from the faeces of two adult males of the two-toed sloth (Choloepus didactylus) housed in Parco Natura Viva, in Italy.
33052806	5	19	theme	20436T	727:732	arg1	strain					689:694	the type strain	680:694	the type strain of Bifidobacterium dentium DSM 20436T	680:732	kashiwanohense DSM 21854T (95.4 %) was the closest neighbour to strain in Cluster I (BRDM 6T), whereas the type strain of Bifidobacterium dentium DSM 20436T (values were in the range of 98‒99.8 %) was the closest neighbour to the other six strains in Cluster II.
33052806	5	19	theme	20436T	727:732	arg1	neighbour					790:798	the closest neighbour	778:798	the closest neighbour to the other six strains in Cluster II	778:837	kashiwanohense DSM 21854T (95.4 %) was the closest neighbour to strain in Cluster I (BRDM 6T), whereas the type strain of Bifidobacterium dentium DSM 20436T (values were in the range of 98‒99.8 %) was the closest neighbour to the other six strains in Cluster II.
33052806	4	20	theme	Bifidobacterium	542:556	arg1	subsp					570:574	Bifidobacterium catenulatum subsp	542:574	Bifidobacterium catenulatum subsp	542:574	On the basis of 16S rRNA gene sequence similarity, the type strain of Bifidobacterium catenulatum subsp.
33052806	7	21	theme	choloepi	1222:1229	arg1	sp					1231:1232	Bifidobacterium choloepi sp	1206:1232	Bifidobacterium choloepi sp	1206:1232	Therefore, genotyping based on the genome sequence of the strain BRDM 6T combined with phenotypic analyses clearly revealed that the strain BRDM 6T represents a novel species for which the names Bifidobacterium choloepi sp.
33052806	6	22	theme	BRDM	888:891	arg1	6T					893:894	BRDM 6T	888:894	BRDM 6T	888:894	The average nucleotide identity (ANI) values of BRDM 6T and of strains in Cluster II with the closely related type strains were 76.0 and 98.9 % (mean value) respectively.
33052806	0	23	theme	taxon	101:105	arg1	sp					132:133	the novel taxon Bifidobacterium choloepi sp	91:133	the novel taxon Bifidobacterium choloepi sp	91:133	Bifidobacteria in two-toed sloths (Choloepus didactylus): phylogenetic characterization of the novel taxon Bifidobacterium choloepi sp.
33052806	5	24	from	neighbour	628:636	arg1	I					659:659	Cluster I	651:659	Cluster I (BRDM 6T)	651:669	kashiwanohense DSM 21854T (95.4 %) was the closest neighbour to strain in Cluster I (BRDM 6T), whereas the type strain of Bifidobacterium dentium DSM 20436T (values were in the range of 98‒99.8 %) was the closest neighbour to the other six strains in Cluster II.
33052806	5	24	from	neighbour	628:636	arg1	6T					667:668	BRDM 6T	662:668	BRDM 6T	662:668	kashiwanohense DSM 21854T (95.4 %) was the closest neighbour to strain in Cluster I (BRDM 6T), whereas the type strain of Bifidobacterium dentium DSM 20436T (values were in the range of 98‒99.8 %) was the closest neighbour to the other six strains in Cluster II.
33052806	7	25	dep	sp	1231:1232	arg1	names					1200:1204	the names	1196:1204	the names	1196:1204	Therefore, genotyping based on the genome sequence of the strain BRDM 6T combined with phenotypic analyses clearly revealed that the strain BRDM 6T represents a novel species for which the names Bifidobacterium choloepi sp.
33052806	8	26	theme	114053T=BCRC	1254:1265	arg1	81222T					1267:1272	BRDM 6T=NBRC 114053T=BCRC 81222T	1241:1272	BRDM 6T=NBRC 114053T=BCRC 81222T	1241:1272	nov. (BRDM 6T=NBRC 114053T=BCRC 81222T) is proposed.
33052806	8	26	theme	114053T=BCRC	1254:1265	arg1	nov					1235:1237	nov	1235:1237	nov	1235:1237	nov. (BRDM 6T=NBRC 114053T=BCRC 81222T) is proposed.
33052806	2	27	dep	sloth	236:240	arg1	didactylus					253:262	Choloepus didactylus	243:262	Choloepus didactylus	243:262	Seven bifidobacterial strains were isolated from the faeces of two adult males of the two-toed sloth (Choloepus didactylus) housed in Parco Natura Viva, in Italy.
33052806	0	28	theme	phylogenetic	58:69	arg1	characterization					71:86	phylogenetic characterization	58:86	Bifidobacteria in two-toed sloths (Choloepus didactylus): phylogenetic characterization of the novel taxon Bifidobacterium choloepi sp.	0:134	Bifidobacteria in two-toed sloths (Choloepus didactylus): phylogenetic characterization of the novel taxon Bifidobacterium choloepi sp.
33052806	3	29	theme	housekeeping	358:369	arg1	genes					403:407	five housekeeping (hsp60, rpoB, clpC, dnaJ, dnaG) genes	353:407	five housekeeping (hsp60, rpoB, clpC, dnaJ, dnaG) genes	353:407	Comparative sequence analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dnaG) genes revealed that these strains were classified into two clusters.
33052806	7	30	theme	Bifidobacterium	1206:1220	arg1	sp					1231:1232	Bifidobacterium choloepi sp	1206:1232	Bifidobacterium choloepi sp	1206:1232	Therefore, genotyping based on the genome sequence of the strain BRDM 6T combined with phenotypic analyses clearly revealed that the strain BRDM 6T represents a novel species for which the names Bifidobacterium choloepi sp.
33052806	6	31	theme	nucleotide	852:861	arg1	values					878:883	The average nucleotide identity (ANI) values	840:883	The average nucleotide identity (ANI) values of BRDM 6T and of strains in Cluster II with the closely related type strains	840:961	The average nucleotide identity (ANI) values of BRDM 6T and of strains in Cluster II with the closely related type strains were 76.0 and 98.9 % (mean value) respectively.
33052806	6	31	theme	nucleotide	852:861	arg1	%					982:982	76.0 and 98.9 %	968:982	76.0 and 98.9 % (mean value)	968:995	The average nucleotide identity (ANI) values of BRDM 6T and of strains in Cluster II with the closely related type strains were 76.0 and 98.9 % (mean value) respectively.
33052806	5	32	from	neighbour	790:798	arg1	Cluster					828:834	Cluster II	828:837	Cluster II	828:837	kashiwanohense DSM 21854T (95.4 %) was the closest neighbour to strain in Cluster I (BRDM 6T), whereas the type strain of Bifidobacterium dentium DSM 20436T (values were in the range of 98‒99.8 %) was the closest neighbour to the other six strains in Cluster II.
33052806	5	33	theme	Bifidobacterium	699:713	arg1	20436T					727:732	Bifidobacterium dentium DSM 20436T	699:732	Bifidobacterium dentium DSM 20436T	699:732	kashiwanohense DSM 21854T (95.4 %) was the closest neighbour to strain in Cluster I (BRDM 6T), whereas the type strain of Bifidobacterium dentium DSM 20436T (values were in the range of 98‒99.8 %) was the closest neighbour to the other six strains in Cluster II.
33052806	0	34	theme	sp	132:133	arg1	characterization					71:86	phylogenetic characterization	58:86	Bifidobacteria in two-toed sloths (Choloepus didactylus): phylogenetic characterization of the novel taxon Bifidobacterium choloepi sp.	0:134	Bifidobacteria in two-toed sloths (Choloepus didactylus): phylogenetic characterization of the novel taxon Bifidobacterium choloepi sp.
33052806	6	35	from	strains	903:909	arg1	Cluster					914:920	Cluster II	914:923	Cluster II	914:923	The average nucleotide identity (ANI) values of BRDM 6T and of strains in Cluster II with the closely related type strains were 76.0 and 98.9 % (mean value) respectively.
33052806	5	36	theme	other	807:811	arg1	strains					817:823	the other six strains	803:823	the other six strains	803:823	kashiwanohense DSM 21854T (95.4 %) was the closest neighbour to strain in Cluster I (BRDM 6T), whereas the type strain of Bifidobacterium dentium DSM 20436T (values were in the range of 98‒99.8 %) was the closest neighbour to the other six strains in Cluster II.
33052806	4	37	theme	catenulatum	558:568	arg1	subsp					570:574	Bifidobacterium catenulatum subsp	542:574	Bifidobacterium catenulatum subsp	542:574	On the basis of 16S rRNA gene sequence similarity, the type strain of Bifidobacterium catenulatum subsp.
33052806	0	38	theme	two-toed	18:25	arg1	sloths					27:32	two-toed sloths	18:32	two-toed sloths (Choloepus didactylus)	18:55	Bifidobacteria in two-toed sloths (Choloepus didactylus): phylogenetic characterization of the novel taxon Bifidobacterium choloepi sp.
33052806	4	39	theme	rRNA	492:495	arg1	similarity					511:520	16S rRNA gene sequence similarity	488:520	16S rRNA gene sequence similarity	488:520	On the basis of 16S rRNA gene sequence similarity, the type strain of Bifidobacterium catenulatum subsp.
33052806	0	40	theme	choloepi	123:130	arg1	sp					132:133	the novel taxon Bifidobacterium choloepi sp	91:133	the novel taxon Bifidobacterium choloepi sp	91:133	Bifidobacteria in two-toed sloths (Choloepus didactylus): phylogenetic characterization of the novel taxon Bifidobacterium choloepi sp.
33052806	7	41	theme	genome	1046:1051	arg1	sequence					1053:1060	the genome sequence	1042:1060	the genome sequence of the strain BRDM 6T combined with phenotypic analyses	1042:1116	Therefore, genotyping based on the genome sequence of the strain BRDM 6T combined with phenotypic analyses clearly revealed that the strain BRDM 6T represents a novel species for which the names Bifidobacterium choloepi sp.
33052806	3	42	theme	genes	403:407	arg1	analysis					325:332	Comparative sequence analysis	304:332	Comparative sequence analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dnaG) genes	304:407	Comparative sequence analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dnaG) genes revealed that these strains were classified into two clusters.
33052806	4	43	theme	16S	488:490	arg1	similarity					511:520	16S rRNA gene sequence similarity	488:520	16S rRNA gene sequence similarity	488:520	On the basis of 16S rRNA gene sequence similarity, the type strain of Bifidobacterium catenulatum subsp.
33052806	4	44	dep	similarity	511:520	arg1	the					475:477	the	475:477	the	475:477	On the basis of 16S rRNA gene sequence similarity, the type strain of Bifidobacterium catenulatum subsp.
33052806	4	44	dep	similarity	511:520	arg1	basis					479:483	basis	479:483	basis	479:483	On the basis of 16S rRNA gene sequence similarity, the type strain of Bifidobacterium catenulatum subsp.
33052806	2	45	theme	two-toed	227:234	arg1	sloth					236:240	the two-toed sloth	223:240	the two-toed sloth (Choloepus didactylus) housed in Parco Natura Viva, in Italy	223:301	Seven bifidobacterial strains were isolated from the faeces of two adult males of the two-toed sloth (Choloepus didactylus) housed in Parco Natura Viva, in Italy.
33052806	6	46	theme	average	844:850	arg1	values					878:883	The average nucleotide identity (ANI) values	840:883	The average nucleotide identity (ANI) values of BRDM 6T and of strains in Cluster II with the closely related type strains	840:961	The average nucleotide identity (ANI) values of BRDM 6T and of strains in Cluster II with the closely related type strains were 76.0 and 98.9 % (mean value) respectively.
33052806	6	46	theme	average	844:850	arg1	%					982:982	76.0 and 98.9 %	968:982	76.0 and 98.9 % (mean value)	968:995	The average nucleotide identity (ANI) values of BRDM 6T and of strains in Cluster II with the closely related type strains were 76.0 and 98.9 % (mean value) respectively.
33052806	8	47	theme	6T=NBRC	1246:1252	arg1	81222T					1267:1272	BRDM 6T=NBRC 114053T=BCRC 81222T	1241:1272	BRDM 6T=NBRC 114053T=BCRC 81222T	1241:1272	nov. (BRDM 6T=NBRC 114053T=BCRC 81222T) is proposed.
33052806	8	47	theme	6T=NBRC	1246:1252	arg1	nov					1235:1237	nov	1235:1237	nov	1235:1237	nov. (BRDM 6T=NBRC 114053T=BCRC 81222T) is proposed.
33052806	7	48	theme	novel	1172:1176	arg1	species					1178:1184	a novel species	1170:1184	a novel species for which the names Bifidobacterium choloepi sp	1170:1232	Therefore, genotyping based on the genome sequence of the strain BRDM 6T combined with phenotypic analyses clearly revealed that the strain BRDM 6T represents a novel species for which the names Bifidobacterium choloepi sp.
33052806	2	49	attach	isolated	176:183	arg2	strains					163:169	Seven bifidobacterial strains	141:169	Seven bifidobacterial strains	141:169	Seven bifidobacterial strains were isolated from the faeces of two adult males of the two-toed sloth (Choloepus didactylus) housed in Parco Natura Viva, in Italy.
33052806	2	49	attach	isolated	176:183	arg1	faeces					194:199	the faeces	190:199	the faeces of two adult males of the two-toed sloth (Choloepus didactylus) housed in Parco Natura Viva, in Italy	190:301	Seven bifidobacterial strains were isolated from the faeces of two adult males of the two-toed sloth (Choloepus didactylus) housed in Parco Natura Viva, in Italy.
33052806	3	50	theme	16S	337:339	arg1	rRNA					341:344	16S rRNA	337:344	16S rRNA	337:344	Comparative sequence analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dnaG) genes revealed that these strains were classified into two clusters.
33052806	5	51	theme	BRDM	662:665	arg1	I					659:659	Cluster I	651:659	Cluster I (BRDM 6T)	651:669	kashiwanohense DSM 21854T (95.4 %) was the closest neighbour to strain in Cluster I (BRDM 6T), whereas the type strain of Bifidobacterium dentium DSM 20436T (values were in the range of 98‒99.8 %) was the closest neighbour to the other six strains in Cluster II.
33052806	5	51	theme	BRDM	662:665	arg1	6T					667:668	BRDM 6T	662:668	BRDM 6T	662:668	kashiwanohense DSM 21854T (95.4 %) was the closest neighbour to strain in Cluster I (BRDM 6T), whereas the type strain of Bifidobacterium dentium DSM 20436T (values were in the range of 98‒99.8 %) was the closest neighbour to the other six strains in Cluster II.
33052806	6	52	theme	related	942:948	arg1	strains					955:961	the closely related type strains	930:961	the closely related type strains	930:961	The average nucleotide identity (ANI) values of BRDM 6T and of strains in Cluster II with the closely related type strains were 76.0 and 98.9 % (mean value) respectively.
33052806	7	53	theme	BRDM	1076:1079	arg1	6T					1081:1082	the strain BRDM 6T	1065:1082	the strain BRDM 6T combined with phenotypic analyses	1065:1116	Therefore, genotyping based on the genome sequence of the strain BRDM 6T combined with phenotypic analyses clearly revealed that the strain BRDM 6T represents a novel species for which the names Bifidobacterium choloepi sp.
33052806	5	54	theme	type	684:687	arg1	strain					689:694	the type strain	680:694	the type strain of Bifidobacterium dentium DSM 20436T	680:732	kashiwanohense DSM 21854T (95.4 %) was the closest neighbour to strain in Cluster I (BRDM 6T), whereas the type strain of Bifidobacterium dentium DSM 20436T (values were in the range of 98‒99.8 %) was the closest neighbour to the other six strains in Cluster II.
33052806	5	54	theme	type	684:687	arg1	neighbour					790:798	the closest neighbour	778:798	the closest neighbour to the other six strains in Cluster II	778:837	kashiwanohense DSM 21854T (95.4 %) was the closest neighbour to strain in Cluster I (BRDM 6T), whereas the type strain of Bifidobacterium dentium DSM 20436T (values were in the range of 98‒99.8 %) was the closest neighbour to the other six strains in Cluster II.
33052806	2	55	theme	adult	208:212	arg1	males					214:218	two adult males	204:218	two adult males of the two-toed sloth (Choloepus didactylus) housed in Parco Natura Viva, in Italy	204:301	Seven bifidobacterial strains were isolated from the faeces of two adult males of the two-toed sloth (Choloepus didactylus) housed in Parco Natura Viva, in Italy.
33052806	2	56	theme	males	214:218	arg1	faeces					194:199	the faeces	190:199	the faeces of two adult males of the two-toed sloth (Choloepus didactylus) housed in Parco Natura Viva, in Italy	190:301	Seven bifidobacterial strains were isolated from the faeces of two adult males of the two-toed sloth (Choloepus didactylus) housed in Parco Natura Viva, in Italy.
33052806	6	57	theme	type	950:953	arg1	strains					955:961	the closely related type strains	930:961	the closely related type strains	930:961	The average nucleotide identity (ANI) values of BRDM 6T and of strains in Cluster II with the closely related type strains were 76.0 and 98.9 % (mean value) respectively.
33052806	0	58	dep	Bifidobacteria	0:13	arg1	characterization					71:86	phylogenetic characterization	58:86	Bifidobacteria in two-toed sloths (Choloepus didactylus): phylogenetic characterization of the novel taxon Bifidobacterium choloepi sp.	0:134	Bifidobacteria in two-toed sloths (Choloepus didactylus): phylogenetic characterization of the novel taxon Bifidobacterium choloepi sp.
33052806	4	59	dep	strain	532:537	arg1	similarity					511:520	16S rRNA gene sequence similarity	488:520	16S rRNA gene sequence similarity	488:520	On the basis of 16S rRNA gene sequence similarity, the type strain of Bifidobacterium catenulatum subsp.
33052806	0	60	dep	sloths	27:32	arg1	didactylus					45:54	Choloepus didactylus	35:54	Choloepus didactylus	35:54	Bifidobacteria in two-toed sloths (Choloepus didactylus): phylogenetic characterization of the novel taxon Bifidobacterium choloepi sp.
33052806	3	61	dep	genes	403:407	arg1	hsp60					372:376	hsp60	372:376	hsp60	372:376	Comparative sequence analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dnaG) genes revealed that these strains were classified into two clusters.
33052806	3	61	dep	genes	403:407	arg1	dnaJ					391:394	dnaJ	391:394	dnaJ	391:394	Comparative sequence analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dnaG) genes revealed that these strains were classified into two clusters.
33052806	3	61	dep	genes	403:407	arg1	clpC					385:388	clpC	385:388	clpC	385:388	Comparative sequence analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dnaG) genes revealed that these strains were classified into two clusters.
33052806	3	61	dep	genes	403:407	arg1	rpoB					379:382	rpoB	379:382	rpoB	379:382	Comparative sequence analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dnaG) genes revealed that these strains were classified into two clusters.
33052806	3	61	dep	genes	403:407	arg1	dnaG					397:400	dnaG	397:400	dnaG	397:400	Comparative sequence analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dnaG) genes revealed that these strains were classified into two clusters.
33052806	5	62	theme	closest	782:788	arg1	strain					689:694	the type strain	680:694	the type strain of Bifidobacterium dentium DSM 20436T	680:732	kashiwanohense DSM 21854T (95.4 %) was the closest neighbour to strain in Cluster I (BRDM 6T), whereas the type strain of Bifidobacterium dentium DSM 20436T (values were in the range of 98‒99.8 %) was the closest neighbour to the other six strains in Cluster II.
33052806	5	62	theme	closest	782:788	arg1	neighbour					790:798	the closest neighbour	778:798	the closest neighbour to the other six strains in Cluster II	778:837	kashiwanohense DSM 21854T (95.4 %) was the closest neighbour to strain in Cluster I (BRDM 6T), whereas the type strain of Bifidobacterium dentium DSM 20436T (values were in the range of 98‒99.8 %) was the closest neighbour to the other six strains in Cluster II.
33052806	4	63	theme	type	527:530	arg1	strain					532:537	the type strain	523:537	the type strain of Bifidobacterium catenulatum subsp	523:574	On the basis of 16S rRNA gene sequence similarity, the type strain of Bifidobacterium catenulatum subsp.
33052806	8	64	theme	BRDM	1241:1244	arg1	81222T					1267:1272	BRDM 6T=NBRC 114053T=BCRC 81222T	1241:1272	BRDM 6T=NBRC 114053T=BCRC 81222T	1241:1272	nov. (BRDM 6T=NBRC 114053T=BCRC 81222T) is proposed.
33052806	8	64	theme	BRDM	1241:1244	arg1	nov					1235:1237	nov	1235:1237	nov	1235:1237	nov. (BRDM 6T=NBRC 114053T=BCRC 81222T) is proposed.
33052806	7	65	theme	6T	1081:1082	arg1	sequence					1053:1060	the genome sequence	1042:1060	the genome sequence of the strain BRDM 6T combined with phenotypic analyses	1042:1116	Therefore, genotyping based on the genome sequence of the strain BRDM 6T combined with phenotypic analyses clearly revealed that the strain BRDM 6T represents a novel species for which the names Bifidobacterium choloepi sp.
33052806	5	66	theme	dentium	715:721	arg1	20436T					727:732	Bifidobacterium dentium DSM 20436T	699:732	Bifidobacterium dentium DSM 20436T	699:732	kashiwanohense DSM 21854T (95.4 %) was the closest neighbour to strain in Cluster I (BRDM 6T), whereas the type strain of Bifidobacterium dentium DSM 20436T (values were in the range of 98‒99.8 %) was the closest neighbour to the other six strains in Cluster II.
33052806	7	67	theme	strain	1069:1074	arg1	6T					1081:1082	the strain BRDM 6T	1065:1082	the strain BRDM 6T combined with phenotypic analyses	1065:1116	Therefore, genotyping based on the genome sequence of the strain BRDM 6T combined with phenotypic analyses clearly revealed that the strain BRDM 6T represents a novel species for which the names Bifidobacterium choloepi sp.
33052806	6	68	theme	ANI	873:875	arg1	values					878:883	The average nucleotide identity (ANI) values	840:883	The average nucleotide identity (ANI) values of BRDM 6T and of strains in Cluster II with the closely related type strains	840:961	The average nucleotide identity (ANI) values of BRDM 6T and of strains in Cluster II with the closely related type strains were 76.0 and 98.9 % (mean value) respectively.
33052806	6	68	theme	ANI	873:875	arg1	%					982:982	76.0 and 98.9 %	968:982	76.0 and 98.9 % (mean value)	968:995	The average nucleotide identity (ANI) values of BRDM 6T and of strains in Cluster II with the closely related type strains were 76.0 and 98.9 % (mean value) respectively.
33052806	3	69	theme	Comparative	304:314	arg1	analysis					325:332	Comparative sequence analysis	304:332	Comparative sequence analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dnaG) genes	304:407	Comparative sequence analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dnaG) genes revealed that these strains were classified into two clusters.
33052806	4	70	theme	subsp	570:574	arg1	strain					532:537	the type strain	523:537	the type strain of Bifidobacterium catenulatum subsp	523:574	On the basis of 16S rRNA gene sequence similarity, the type strain of Bifidobacterium catenulatum subsp.
33052806	7	71	theme	strain	1144:1149	arg1	6T					1156:1157	the strain BRDM 6T	1140:1157	the strain BRDM 6T	1140:1157	Therefore, genotyping based on the genome sequence of the strain BRDM 6T combined with phenotypic analyses clearly revealed that the strain BRDM 6T represents a novel species for which the names Bifidobacterium choloepi sp.
33052806	5	72	theme	DSM	723:725	arg1	20436T					727:732	Bifidobacterium dentium DSM 20436T	699:732	Bifidobacterium dentium DSM 20436T	699:732	kashiwanohense DSM 21854T (95.4 %) was the closest neighbour to strain in Cluster I (BRDM 6T), whereas the type strain of Bifidobacterium dentium DSM 20436T (values were in the range of 98‒99.8 %) was the closest neighbour to the other six strains in Cluster II.
33052806	6	73	theme	strains	903:909	arg1	values					878:883	The average nucleotide identity (ANI) values	840:883	The average nucleotide identity (ANI) values of BRDM 6T and of strains in Cluster II with the closely related type strains	840:961	The average nucleotide identity (ANI) values of BRDM 6T and of strains in Cluster II with the closely related type strains were 76.0 and 98.9 % (mean value) respectively.
33052806	6	73	theme	strains	903:909	arg1	%					982:982	76.0 and 98.9 %	968:982	76.0 and 98.9 % (mean value)	968:995	The average nucleotide identity (ANI) values of BRDM 6T and of strains in Cluster II with the closely related type strains were 76.0 and 98.9 % (mean value) respectively.
33052806	3	74	theme	sequence	316:323	arg1	analysis					325:332	Comparative sequence analysis	304:332	Comparative sequence analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dnaG) genes	304:407	Comparative sequence analysis of 16S rRNA and of five housekeeping (hsp60, rpoB, clpC, dnaJ, dnaG) genes revealed that these strains were classified into two clusters.
33052806	2	75	theme	sloth	236:240	arg1	males					214:218	two adult males	204:218	two adult males of the two-toed sloth (Choloepus didactylus) housed in Parco Natura Viva, in Italy	204:301	Seven bifidobacterial strains were isolated from the faeces of two adult males of the two-toed sloth (Choloepus didactylus) housed in Parco Natura Viva, in Italy.
33052806	5	76	theme	Cluster	651:657	arg1	I					659:659	Cluster I	651:659	Cluster I (BRDM 6T)	651:669	kashiwanohense DSM 21854T (95.4 %) was the closest neighbour to strain in Cluster I (BRDM 6T), whereas the type strain of Bifidobacterium dentium DSM 20436T (values were in the range of 98‒99.8 %) was the closest neighbour to the other six strains in Cluster II.
33052806	5	76	theme	Cluster	651:657	arg1	6T					667:668	BRDM 6T	662:668	BRDM 6T	662:668	kashiwanohense DSM 21854T (95.4 %) was the closest neighbour to strain in Cluster I (BRDM 6T), whereas the type strain of Bifidobacterium dentium DSM 20436T (values were in the range of 98‒99.8 %) was the closest neighbour to the other six strains in Cluster II.
32213962	6	0	theme	trypsin	1165:1171	arg1	inhibitors					1173:1182	trypsin inhibitors	1165:1182	trypsin inhibitors (95.5%)	1165:1190	When applying the DIC treatment, the content of phytates (23-29%), saponins (44%) and oligosaccharides increased in bean sprouts and decreased phenolic compounds (4-14%), tannins (23% to 72%), and trypsin inhibitors (95.5%), according to the pressure and time conditions applied.
32213962	6	0	theme	trypsin	1165:1171	arg1	%					1189:1189	95.5%	1185:1189	95.5%	1185:1189	When applying the DIC treatment, the content of phytates (23-29%), saponins (44%) and oligosaccharides increased in bean sprouts and decreased phenolic compounds (4-14%), tannins (23% to 72%), and trypsin inhibitors (95.5%), according to the pressure and time conditions applied.
32213962	4	1	theme	non-nutritional	644:658	arg1	composition					660:670	the non-nutritional composition	640:670	the non-nutritional composition of bean seeds and sprouts by DIC treatment	640:713	The objective of this work was to evaluate the change in the non-nutritional composition of bean seeds and sprouts by DIC treatment.
32213962	3	2	theme	DIC	466:468	arg1	acronym					478:484	DIC, French acronym	466:484	DIC, French acronym of Détente Instantanée Contrôlée	466:517	Germination and treatment by controlled pressure-drop (DIC, French acronym of Détente Instantanée Contrôlée) are methods that modify the concentration of these components.
32213962	0	3	theme	Seeds	79:83	arg1	Compounds					66:74	the Non-Nutritional Compounds	46:74	the Non-Nutritional Compounds of Seeds and Sprouts of Common Black Bean (Phaseolus vulgaris L.)	46:140	Effect of Instant Controlled Pressure-Drop on the Non-Nutritional Compounds of Seeds and Sprouts of Common Black Bean (Phaseolus vulgaris L.).
32213962	0	4	dep	Bean	113:116	arg1	vulgaris					129:136	Phaseolus vulgaris	119:136	Phaseolus vulgaris L.	119:139	Effect of Instant Controlled Pressure-Drop on the Non-Nutritional Compounds of Seeds and Sprouts of Common Black Bean (Phaseolus vulgaris L.).
32213962	6	5	theme	time	1223:1226	arg1	conditions					1228:1237	the pressure and time conditions	1206:1237	conditions	1228:1237	When applying the DIC treatment, the content of phytates (23-29%), saponins (44%) and oligosaccharides increased in bean sprouts and decreased phenolic compounds (4-14%), tannins (23% to 72%), and trypsin inhibitors (95.5%), according to the pressure and time conditions applied.
32213962	2	6	theme	nutrients	337:345	arg1	assimilation					316:327	the assimilation	312:327	the assimilation of some nutrients	312:345	However, its nutritional value may be affected by the presence of non-nutritional compounds, which decrease the assimilation of some nutrients; however, at low concentrations, they show a beneficial effect.
32213962	0	7	from	Effect	0:5	arg1	Compounds					66:74	the Non-Nutritional Compounds	46:74	the Non-Nutritional Compounds of Seeds and Sprouts of Common Black Bean (Phaseolus vulgaris L.)	46:140	Effect of Instant Controlled Pressure-Drop on the Non-Nutritional Compounds of Seeds and Sprouts of Common Black Bean (Phaseolus vulgaris L.).
32213962	5	8	theme	saponins	875:882	arg1	quantity					863:870	the quantity	859:870	the quantity of saponins (65.7%)	859:890	The results show that with the germination, the concentration of phenolic and tannin compounds increased 99% and 73%, respectively, as well as the quantity of saponins (65.7%), while phytates and trypsin inhibitors decreased 26% and 42%, respectively.
32213962	5	8	theme	saponins	875:882	arg1	%					831:831	73%	829:831	73%	829:831	The results show that with the germination, the concentration of phenolic and tannin compounds increased 99% and 73%, respectively, as well as the quantity of saponins (65.7%), while phytates and trypsin inhibitors decreased 26% and 42%, respectively.
32213962	5	8	theme	saponins	875:882	arg1	%					889:889	65.7%	885:889	65.7%	885:889	The results show that with the germination, the concentration of phenolic and tannin compounds increased 99% and 73%, respectively, as well as the quantity of saponins (65.7%), while phytates and trypsin inhibitors decreased 26% and 42%, respectively.
32213962	5	8	theme	saponins	875:882	arg1	saponins					875:882	saponins	875:882	saponins	875:882	The results show that with the germination, the concentration of phenolic and tannin compounds increased 99% and 73%, respectively, as well as the quantity of saponins (65.7%), while phytates and trypsin inhibitors decreased 26% and 42%, respectively.
32213962	5	8	theme	saponins	875:882	arg1	%					823:823	99%	821:823	99%	821:823	The results show that with the germination, the concentration of phenolic and tannin compounds increased 99% and 73%, respectively, as well as the quantity of saponins (65.7%), while phytates and trypsin inhibitors decreased 26% and 42%, respectively.
32213962	4	9	from	change	630:635	arg1	composition					660:670	the non-nutritional composition	640:670	the non-nutritional composition of bean seeds and sprouts by DIC treatment	640:713	The objective of this work was to evaluate the change in the non-nutritional composition of bean seeds and sprouts by DIC treatment.
32213962	0	10	theme	Sprouts	89:95	arg1	Compounds					66:74	the Non-Nutritional Compounds	46:74	the Non-Nutritional Compounds of Seeds and Sprouts of Common Black Bean (Phaseolus vulgaris L.)	46:140	Effect of Instant Controlled Pressure-Drop on the Non-Nutritional Compounds of Seeds and Sprouts of Common Black Bean (Phaseolus vulgaris L.).
32213962	6	11	theme	phenolic	1111:1118	arg1	compounds					1120:1128	phenolic compounds	1111:1128	phenolic compounds (4-14%)	1111:1136	When applying the DIC treatment, the content of phytates (23-29%), saponins (44%) and oligosaccharides increased in bean sprouts and decreased phenolic compounds (4-14%), tannins (23% to 72%), and trypsin inhibitors (95.5%), according to the pressure and time conditions applied.
32213962	6	11	theme	phenolic	1111:1118	arg1	%					1135:1135	4-14%	1131:1135	4-14%	1131:1135	When applying the DIC treatment, the content of phytates (23-29%), saponins (44%) and oligosaccharides increased in bean sprouts and decreased phenolic compounds (4-14%), tannins (23% to 72%), and trypsin inhibitors (95.5%), according to the pressure and time conditions applied.
32213962	3	12	theme	Contrôlée	509:517	arg1	acronym					478:484	DIC, French acronym	466:484	DIC, French acronym of Détente Instantanée Contrôlée	466:517	Germination and treatment by controlled pressure-drop (DIC, French acronym of Détente Instantanée Contrôlée) are methods that modify the concentration of these components.
32213962	4	13	theme	bean	675:678	arg1	seeds					680:684	bean seeds	675:684	bean seeds	675:684	The objective of this work was to evaluate the change in the non-nutritional composition of bean seeds and sprouts by DIC treatment.
32213962	0	14	theme	Black	107:111	arg1	Bean					113:116	Common Black Bean	100:116	Common Black Bean (Phaseolus vulgaris L.)	100:140	Effect of Instant Controlled Pressure-Drop on the Non-Nutritional Compounds of Seeds and Sprouts of Common Black Bean (Phaseolus vulgaris L.).
32213962	5	15	theme	phenolic	781:788	arg1	compounds					801:809	phenolic and tannin compounds	781:809	phenolic and tannin compounds	781:809	The results show that with the germination, the concentration of phenolic and tannin compounds increased 99% and 73%, respectively, as well as the quantity of saponins (65.7%), while phytates and trypsin inhibitors decreased 26% and 42%, respectively.
32213962	4	16	theme	sprouts	690:696	arg1	composition					660:670	the non-nutritional composition	640:670	the non-nutritional composition of bean seeds and sprouts by DIC treatment	640:713	The objective of this work was to evaluate the change in the non-nutritional composition of bean seeds and sprouts by DIC treatment.
32213962	3	17	dep	pressure-drop	451:463	arg1	acronym					478:484	DIC, French acronym	466:484	DIC, French acronym of Détente Instantanée Contrôlée	466:517	Germination and treatment by controlled pressure-drop (DIC, French acronym of Détente Instantanée Contrôlée) are methods that modify the concentration of these components.
32213962	5	18	theme	compounds	801:809	arg1	concentration					764:776	the concentration	760:776	the concentration of phenolic and tannin compounds	760:809	The results show that with the germination, the concentration of phenolic and tannin compounds increased 99% and 73%, respectively, as well as the quantity of saponins (65.7%), while phytates and trypsin inhibitors decreased 26% and 42%, respectively.
32213962	0	19	theme	Common	100:105	arg1	Bean					113:116	Common Black Bean	100:116	Common Black Bean (Phaseolus vulgaris L.)	100:140	Effect of Instant Controlled Pressure-Drop on the Non-Nutritional Compounds of Seeds and Sprouts of Common Black Bean (Phaseolus vulgaris L.).
32213962	1	20	theme	common	147:152	arg1	bean					154:157	The common bean	143:157	The common bean	143:157	The common bean is an important caloric-protein food source.
32213962	1	20	theme	common	147:152	arg1	source					196:201	an important caloric-protein food source	162:201	an important caloric-protein food source	162:201	The common bean is an important caloric-protein food source.
32213962	6	21	theme	pressure	1210:1217	arg1	conditions					1228:1237	the pressure and time conditions	1206:1237	conditions	1228:1237	When applying the DIC treatment, the content of phytates (23-29%), saponins (44%) and oligosaccharides increased in bean sprouts and decreased phenolic compounds (4-14%), tannins (23% to 72%), and trypsin inhibitors (95.5%), according to the pressure and time conditions applied.
32213962	7	22	theme	compounds	1389:1397	arg1	way					1274:1276	the way	1270:1276	the way	1270:1276	This technology opens the way to new perspectives, especially to more effective use of legumes as a source of vegetable protein or bioactive compounds.
32213962	7	22	theme	compounds	1389:1397	arg1	source					1348:1353	a source	1346:1353	a source of vegetable protein or bioactive compounds	1346:1397	This technology opens the way to new perspectives, especially to more effective use of legumes as a source of vegetable protein or bioactive compounds.
32213962	2	23	theme	compounds	286:294	arg1	presence					258:265	the presence	254:265	the presence of non-nutritional compounds, which decrease the assimilation of some nutrients	254:345	However, its nutritional value may be affected by the presence of non-nutritional compounds, which decrease the assimilation of some nutrients; however, at low concentrations, they show a beneficial effect.
32213962	2	24	theme	beneficial	392:401	arg1	effect					403:408	a beneficial effect	390:408	a beneficial effect	390:408	However, its nutritional value may be affected by the presence of non-nutritional compounds, which decrease the assimilation of some nutrients; however, at low concentrations, they show a beneficial effect.
32213962	6	25	theme	bean	1084:1087	arg1	sprouts					1089:1095	bean sprouts	1084:1095	bean sprouts	1084:1095	When applying the DIC treatment, the content of phytates (23-29%), saponins (44%) and oligosaccharides increased in bean sprouts and decreased phenolic compounds (4-14%), tannins (23% to 72%), and trypsin inhibitors (95.5%), according to the pressure and time conditions applied.
32213962	2	26	theme	non-nutritional	270:284	arg1	compounds					286:294	non-nutritional compounds	270:294	non-nutritional compounds	270:294	However, its nutritional value may be affected by the presence of non-nutritional compounds, which decrease the assimilation of some nutrients; however, at low concentrations, they show a beneficial effect.
32213962	6	27	theme	%	1150:1150	arg1	tannins					1139:1145	tannins	1139:1145	tannins (23% to 72%)	1139:1158	When applying the DIC treatment, the content of phytates (23-29%), saponins (44%) and oligosaccharides increased in bean sprouts and decreased phenolic compounds (4-14%), tannins (23% to 72%), and trypsin inhibitors (95.5%), according to the pressure and time conditions applied.
32213962	6	27	theme	%	1150:1150	arg1	%					1157:1157	23% to 72%	1148:1157	23% to 72%	1148:1157	When applying the DIC treatment, the content of phytates (23-29%), saponins (44%) and oligosaccharides increased in bean sprouts and decreased phenolic compounds (4-14%), tannins (23% to 72%), and trypsin inhibitors (95.5%), according to the pressure and time conditions applied.
32213962	0	28	theme	Controlled	18:27	arg1	Pressure-Drop					29:41	Instant Controlled Pressure-Drop	10:41	Instant Controlled Pressure-Drop	10:41	Effect of Instant Controlled Pressure-Drop on the Non-Nutritional Compounds of Seeds and Sprouts of Common Black Bean (Phaseolus vulgaris L.).
32213962	7	29	theme	effective	1318:1326	arg1	use					1328:1330	more effective use	1313:1330	more effective use of legumes	1313:1341	This technology opens the way to new perspectives, especially to more effective use of legumes as a source of vegetable protein or bioactive compounds.
32213962	4	30	theme	DIC	701:703	arg1	treatment					705:713	DIC treatment	701:713	DIC treatment	701:713	The objective of this work was to evaluate the change in the non-nutritional composition of bean seeds and sprouts by DIC treatment.
32213962	0	31	theme	Instant	10:16	arg1	Pressure-Drop					29:41	Instant Controlled Pressure-Drop	10:41	Instant Controlled Pressure-Drop	10:41	Effect of Instant Controlled Pressure-Drop on the Non-Nutritional Compounds of Seeds and Sprouts of Common Black Bean (Phaseolus vulgaris L.).
32213962	7	32	theme	bioactive	1379:1387	arg1	compounds					1389:1397	bioactive compounds	1379:1397	bioactive compounds	1379:1397	This technology opens the way to new perspectives, especially to more effective use of legumes as a source of vegetable protein or bioactive compounds.
32213962	0	33	theme	Bean	113:116	arg1	Seeds					79:83	Seeds	79:83	Seeds	79:83	Effect of Instant Controlled Pressure-Drop on the Non-Nutritional Compounds of Seeds and Sprouts of Common Black Bean (Phaseolus vulgaris L.).
32213962	0	33	theme	Bean	113:116	arg1	Sprouts					89:95	Sprouts	89:95	Sprouts	89:95	Effect of Instant Controlled Pressure-Drop on the Non-Nutritional Compounds of Seeds and Sprouts of Common Black Bean (Phaseolus vulgaris L.).
32213962	1	34	theme	important	165:173	arg1	bean					154:157	The common bean	143:157	The common bean	143:157	The common bean is an important caloric-protein food source.
32213962	1	34	theme	important	165:173	arg1	source					196:201	an important caloric-protein food source	162:201	an important caloric-protein food source	162:201	The common bean is an important caloric-protein food source.
32213962	3	35	dep	DIC	466:468	arg1	French					471:476	French	471:476	French	471:476	Germination and treatment by controlled pressure-drop (DIC, French acronym of Détente Instantanée Contrôlée) are methods that modify the concentration of these components.
32213962	4	36	theme	work	605:608	arg1	objective					587:595	The objective	583:595	The objective of this work	583:608	The objective of this work was to evaluate the change in the non-nutritional composition of bean seeds and sprouts by DIC treatment.
32213962	1	37	theme	caloric-protein	175:189	arg1	bean					154:157	The common bean	143:157	The common bean	143:157	The common bean is an important caloric-protein food source.
32213962	1	37	theme	caloric-protein	175:189	arg1	source					196:201	an important caloric-protein food source	162:201	an important caloric-protein food source	162:201	The common bean is an important caloric-protein food source.
32213962	0	38	theme	Pressure-Drop	29:41	arg1	Effect					0:5	Effect	0:5	Effect of Instant Controlled Pressure-Drop on the Non-Nutritional Compounds of Seeds and Sprouts of Common Black Bean (Phaseolus vulgaris L.).	0:141	Effect of Instant Controlled Pressure-Drop on the Non-Nutritional Compounds of Seeds and Sprouts of Common Black Bean (Phaseolus vulgaris L.).
32213962	7	39	theme	protein	1368:1374	arg1	way					1274:1276	the way	1270:1276	the way	1270:1276	This technology opens the way to new perspectives, especially to more effective use of legumes as a source of vegetable protein or bioactive compounds.
32213962	7	39	theme	protein	1368:1374	arg1	source					1348:1353	a source	1346:1353	a source of vegetable protein or bioactive compounds	1346:1397	This technology opens the way to new perspectives, especially to more effective use of legumes as a source of vegetable protein or bioactive compounds.
32213962	0	40	theme	Phaseolus	119:127	arg1	vulgaris					129:136	Phaseolus vulgaris	119:136	Phaseolus vulgaris L.	119:139	Effect of Instant Controlled Pressure-Drop on the Non-Nutritional Compounds of Seeds and Sprouts of Common Black Bean (Phaseolus vulgaris L.).
32213962	5	41	theme	trypsin	912:918	arg1	inhibitors					920:929	trypsin inhibitors	912:929	trypsin inhibitors	912:929	The results show that with the germination, the concentration of phenolic and tannin compounds increased 99% and 73%, respectively, as well as the quantity of saponins (65.7%), while phytates and trypsin inhibitors decreased 26% and 42%, respectively.
32213962	5	42	theme	tannin	794:799	arg1	compounds					801:809	phenolic and tannin compounds	781:809	phenolic and tannin compounds	781:809	The results show that with the germination, the concentration of phenolic and tannin compounds increased 99% and 73%, respectively, as well as the quantity of saponins (65.7%), while phytates and trypsin inhibitors decreased 26% and 42%, respectively.
32213962	2	43	theme	low	360:362	arg1	concentrations					364:377	low concentrations	360:377	low concentrations	360:377	However, its nutritional value may be affected by the presence of non-nutritional compounds, which decrease the assimilation of some nutrients; however, at low concentrations, they show a beneficial effect.
32213962	0	44	theme	Non-Nutritional	50:64	arg1	Compounds					66:74	the Non-Nutritional Compounds	46:74	the Non-Nutritional Compounds of Seeds and Sprouts of Common Black Bean (Phaseolus vulgaris L.)	46:140	Effect of Instant Controlled Pressure-Drop on the Non-Nutritional Compounds of Seeds and Sprouts of Common Black Bean (Phaseolus vulgaris L.).
32213962	6	45	theme	oligosaccharides	1054:1069	arg1	content					1005:1011	the content	1001:1011	the content of phytates (23-29%), saponins (44%) and oligosaccharides	1001:1069	When applying the DIC treatment, the content of phytates (23-29%), saponins (44%) and oligosaccharides increased in bean sprouts and decreased phenolic compounds (4-14%), tannins (23% to 72%), and trypsin inhibitors (95.5%), according to the pressure and time conditions applied.
32213962	6	46	theme	DIC	986:988	arg1	treatment					990:998	the DIC treatment	982:998	the DIC treatment	982:998	When applying the DIC treatment, the content of phytates (23-29%), saponins (44%) and oligosaccharides increased in bean sprouts and decreased phenolic compounds (4-14%), tannins (23% to 72%), and trypsin inhibitors (95.5%), according to the pressure and time conditions applied.
32213962	6	47	dep	%	1157:1157	arg1	to					1152:1153	to	1152:1153	to	1152:1153	When applying the DIC treatment, the content of phytates (23-29%), saponins (44%) and oligosaccharides increased in bean sprouts and decreased phenolic compounds (4-14%), tannins (23% to 72%), and trypsin inhibitors (95.5%), according to the pressure and time conditions applied.
32213962	7	48	theme	vegetable	1358:1366	arg1	protein					1368:1374	vegetable protein	1358:1374	vegetable protein	1358:1374	This technology opens the way to new perspectives, especially to more effective use of legumes as a source of vegetable protein or bioactive compounds.
32213962	7	49	theme	new	1281:1283	arg1	perspectives					1285:1296	new perspectives	1281:1296	new perspectives	1281:1296	This technology opens the way to new perspectives, especially to more effective use of legumes as a source of vegetable protein or bioactive compounds.
32213962	4	50	theme	seeds	680:684	arg1	composition					660:670	the non-nutritional composition	640:670	the non-nutritional composition of bean seeds and sprouts by DIC treatment	640:713	The objective of this work was to evaluate the change in the non-nutritional composition of bean seeds and sprouts by DIC treatment.
32213962	6	51	theme	phytates	1016:1023	arg1	content					1005:1011	the content	1001:1011	the content of phytates (23-29%), saponins (44%) and oligosaccharides	1001:1069	When applying the DIC treatment, the content of phytates (23-29%), saponins (44%) and oligosaccharides increased in bean sprouts and decreased phenolic compounds (4-14%), tannins (23% to 72%), and trypsin inhibitors (95.5%), according to the pressure and time conditions applied.
32213962	6	52	theme	saponins	1035:1042	arg1	content					1005:1011	the content	1001:1011	the content of phytates (23-29%), saponins (44%) and oligosaccharides	1001:1069	When applying the DIC treatment, the content of phytates (23-29%), saponins (44%) and oligosaccharides increased in bean sprouts and decreased phenolic compounds (4-14%), tannins (23% to 72%), and trypsin inhibitors (95.5%), according to the pressure and time conditions applied.
32213962	3	53	theme	controlled	440:449	arg1	pressure-drop					451:463	controlled pressure-drop	440:463	controlled pressure-drop (DIC, French acronym of Détente Instantanée Contrôlée)	440:518	Germination and treatment by controlled pressure-drop (DIC, French acronym of Détente Instantanée Contrôlée) are methods that modify the concentration of these components.
32213962	1	54	theme	food	191:194	arg1	bean					154:157	The common bean	143:157	The common bean	143:157	The common bean is an important caloric-protein food source.
32213962	1	54	theme	food	191:194	arg1	source					196:201	an important caloric-protein food source	162:201	an important caloric-protein food source	162:201	The common bean is an important caloric-protein food source.
32213962	2	55	theme	nutritional	217:227	arg1	value					229:233	its nutritional value	213:233	its nutritional value	213:233	However, its nutritional value may be affected by the presence of non-nutritional compounds, which decrease the assimilation of some nutrients; however, at low concentrations, they show a beneficial effect.
32213962	0	56	dep	vulgaris	129:136	arg1	L					138:138	L	138:138	Phaseolus vulgaris L.	119:139	Effect of Instant Controlled Pressure-Drop on the Non-Nutritional Compounds of Seeds and Sprouts of Common Black Bean (Phaseolus vulgaris L.).
32213962	7	57	theme	legumes	1335:1341	arg1	use					1328:1330	more effective use	1313:1330	more effective use of legumes	1313:1341	This technology opens the way to new perspectives, especially to more effective use of legumes as a source of vegetable protein or bioactive compounds.
32213962	3	58	theme	components	571:580	arg1	concentration					548:560	the concentration	544:560	the concentration of these components	544:580	Germination and treatment by controlled pressure-drop (DIC, French acronym of Détente Instantanée Contrôlée) are methods that modify the concentration of these components.
34863837	0	0	theme	donkey	84:89	arg1	DSP-1					114:118	the major donkey seminal plasma protein DSP-1	74:118	the major donkey seminal plasma protein DSP-1	74:118	Purification, molecular characterization and ligand binding properties of the major donkey seminal plasma protein DSP-1.
34863837	5	1	theme	thermal	1093:1099	arg1	stability					1101:1109	its thermal stability	1089:1109	its thermal stability	1089:1109	Structural and thermal stability studies on DSP-1 employing CD spectroscopy and differential scanning calorimetry showed that the protein unfolds at ~43 °C and binding to phosphorylcholine (PrC) - the head group moiety of choline phospholipids - increases its thermal stability.
34863837	1	2	theme	family	148:153	arg1	proteins					178:185	the major proteins	168:185	the major proteins in many mammalian species including bull, horse and pig	168:241	Fibronectin type-II (FnII) family proteins are the major proteins in many mammalian species including bull, horse and pig.
34863837	1	2	theme	family	148:153	arg1	proteins					155:162	Fibronectin type-II (FnII) family proteins	121:162	Fibronectin type-II (FnII) family proteins	121:162	Fibronectin type-II (FnII) family proteins are the major proteins in many mammalian species including bull, horse and pig.
34863837	3	3	dep	bovine	606:611	arg1	known					627:631	known	627:631	known as BSP-A1/A2	627:644	The amino acid sequence determined by mass spectrometry and computational modeling studies revealed that DSP-1 is homologous to other mammalian seminal plasma proteins, including bovine PDC-109 (also known as BSP-A1/A2) and equine HSP-1/2.
34863837	0	4	theme	major	78:82	arg1	DSP-1					114:118	the major donkey seminal plasma protein DSP-1	74:118	the major donkey seminal plasma protein DSP-1	74:118	Purification, molecular characterization and ligand binding properties of the major donkey seminal plasma protein DSP-1.
34863837	2	5	attach	isolated	311:318	arg2	protein					279:285	a major FnII protein	266:285	a major FnII protein	266:285	In the present study, a major FnII protein has been identified and isolated from donkey (Equus hemionus) seminal plasma, which we refer to as Donkey Seminal Plasma protein-1 (DSP-1).
34863837	2	5	attach	isolated	311:318	arg1	Equus					333:337	Equus	333:337	Equus	333:337	In the present study, a major FnII protein has been identified and isolated from donkey (Equus hemionus) seminal plasma, which we refer to as Donkey Seminal Plasma protein-1 (DSP-1).
34863837	2	5	attach	isolated	311:318	arg1	plasma					357:362	donkey (Equus hemionus) seminal plasma	325:362	donkey (Equus hemionus) seminal plasma	325:362	In the present study, a major FnII protein has been identified and isolated from donkey (Equus hemionus) seminal plasma, which we refer to as Donkey Seminal Plasma protein-1 (DSP-1).
34863837	5	6	from	studies	866:872	arg1	DSP-1					877:881	DSP-1	877:881	DSP-1	877:881	Structural and thermal stability studies on DSP-1 employing CD spectroscopy and differential scanning calorimetry showed that the protein unfolds at ~43 °C and binding to phosphorylcholine (PrC) - the head group moiety of choline phospholipids - increases its thermal stability.
34863837	3	7	theme	acid	437:440	arg1	sequence					442:449	The amino acid sequence	427:449	The amino acid sequence determined by mass spectrometry and computational modeling studies	427:516	The amino acid sequence determined by mass spectrometry and computational modeling studies revealed that DSP-1 is homologous to other mammalian seminal plasma proteins, including bovine PDC-109 (also known as BSP-A1/A2) and equine HSP-1/2.
34863837	0	8	theme	plasma	99:104	arg1	DSP-1					114:118	the major donkey seminal plasma protein DSP-1	74:118	the major donkey seminal plasma protein DSP-1	74:118	Purification, molecular characterization and ligand binding properties of the major donkey seminal plasma protein DSP-1.
34863837	3	9	theme	seminal	571:577	arg1	HSP-1/2					658:664	equine HSP-1/2	651:664	equine HSP-1/2	651:664	The amino acid sequence determined by mass spectrometry and computational modeling studies revealed that DSP-1 is homologous to other mammalian seminal plasma proteins, including bovine PDC-109 (also known as BSP-A1/A2) and equine HSP-1/2.
34863837	3	9	theme	seminal	571:577	arg1	proteins					586:593	other mammalian seminal plasma proteins	555:593	other mammalian seminal plasma proteins	555:593	The amino acid sequence determined by mass spectrometry and computational modeling studies revealed that DSP-1 is homologous to other mammalian seminal plasma proteins, including bovine PDC-109 (also known as BSP-A1/A2) and equine HSP-1/2.
34863837	3	9	theme	seminal	571:577	arg1	bovine					606:611	bovine PDC-109	606:619	bovine PDC-109 (also known as BSP-A1/A2)	606:645	The amino acid sequence determined by mass spectrometry and computational modeling studies revealed that DSP-1 is homologous to other mammalian seminal plasma proteins, including bovine PDC-109 (also known as BSP-A1/A2) and equine HSP-1/2.
34863837	7	10	with	interaction	1256:1266	arg1	membrane					1309:1316	a model cell membrane	1296:1316	a model cell membrane	1296:1316	Further, interaction of DSP-1 with erythrocytes, a model cell membrane, revealed that DSP-1 binding is mediated by a specific interaction with choline phospholipids and results in membrane perturbation, suggesting that binding of this protein to sperm plasma membrane could be physiologically significant.
34863837	7	10	with	interaction	1256:1266	arg1	erythrocytes					1282:1293	erythrocytes	1282:1293	erythrocytes	1282:1293	Further, interaction of DSP-1 with erythrocytes, a model cell membrane, revealed that DSP-1 binding is mediated by a specific interaction with choline phospholipids and results in membrane perturbation, suggesting that binding of this protein to sperm plasma membrane could be physiologically significant.
34863837	6	11	theme	higher	1221:1226	arg1	affinity					1228:1235	100-fold higher affinity	1212:1235	100-fold higher affinity than PrC	1212:1244	Intrinsic fluorescence titrations revealed that DSP-1 recognizes lyso-phosphatidylcholine with over 100-fold higher affinity than PrC.
34863837	6	12	theme	Intrinsic	1112:1120	arg1	titrations					1135:1144	Intrinsic fluorescence titrations	1112:1144	Intrinsic fluorescence titrations	1112:1144	Intrinsic fluorescence titrations revealed that DSP-1 recognizes lyso-phosphatidylcholine with over 100-fold higher affinity than PrC.
34863837	4	13	contain	contains	766:773	arg2	acetylations					784:795	multiple acetylations	775:795	multiple acetylations	775:795	High-resolution LC-MS analysis indicated that the protein is heterogeneously glycosylated and also contains multiple acetylations, occurring in the attached glycans.
34863837	4	13	contain	contains	766:773	arg1	protein					717:723	the protein	713:723	the protein	713:723	High-resolution LC-MS analysis indicated that the protein is heterogeneously glycosylated and also contains multiple acetylations, occurring in the attached glycans.
34863837	0	14	theme	seminal	91:97	arg1	DSP-1					114:118	the major donkey seminal plasma protein DSP-1	74:118	the major donkey seminal plasma protein DSP-1	74:118	Purification, molecular characterization and ligand binding properties of the major donkey seminal plasma protein DSP-1.
34863837	5	15	theme	choline	1055:1061	arg1	phospholipids					1063:1075	choline phospholipids	1055:1075	choline phospholipids	1055:1075	Structural and thermal stability studies on DSP-1 employing CD spectroscopy and differential scanning calorimetry showed that the protein unfolds at ~43 °C and binding to phosphorylcholine (PrC) - the head group moiety of choline phospholipids - increases its thermal stability.
34863837	7	16	with	interaction	1373:1383	arg1	results					1416:1422	results	1416:1422	results	1416:1422	Further, interaction of DSP-1 with erythrocytes, a model cell membrane, revealed that DSP-1 binding is mediated by a specific interaction with choline phospholipids and results in membrane perturbation, suggesting that binding of this protein to sperm plasma membrane could be physiologically significant.
34863837	7	16	with	interaction	1373:1383	arg1	phospholipids					1398:1410	choline phospholipids	1390:1410	choline phospholipids	1390:1410	Further, interaction of DSP-1 with erythrocytes, a model cell membrane, revealed that DSP-1 binding is mediated by a specific interaction with choline phospholipids and results in membrane perturbation, suggesting that binding of this protein to sperm plasma membrane could be physiologically significant.
34863837	3	17	theme	amino	431:435	arg1	sequence					442:449	The amino acid sequence	427:449	The amino acid sequence determined by mass spectrometry and computational modeling studies	427:516	The amino acid sequence determined by mass spectrometry and computational modeling studies revealed that DSP-1 is homologous to other mammalian seminal plasma proteins, including bovine PDC-109 (also known as BSP-A1/A2) and equine HSP-1/2.
34863837	0	18	theme	DSP-1	114:118	arg1	Purification					0:11	Purification	0:11	Purification	0:11	Purification, molecular characterization and ligand binding properties of the major donkey seminal plasma protein DSP-1.
34863837	0	18	theme	DSP-1	114:118	arg1	characterization					24:39	molecular characterization	14:39	molecular characterization	14:39	Purification, molecular characterization and ligand binding properties of the major donkey seminal plasma protein DSP-1.
34863837	0	18	theme	DSP-1	114:118	arg1	properties					60:69	ligand binding properties	45:69	ligand binding properties	45:69	Purification, molecular characterization and ligand binding properties of the major donkey seminal plasma protein DSP-1.
34863837	5	19	theme	phospholipids	1063:1075	arg1	moiety					1045:1050	the head group moiety	1030:1050	the head group moiety of choline phospholipids	1030:1075	Structural and thermal stability studies on DSP-1 employing CD spectroscopy and differential scanning calorimetry showed that the protein unfolds at ~43 °C and binding to phosphorylcholine (PrC) - the head group moiety of choline phospholipids - increases its thermal stability.
34863837	2	20	theme	present	251:257	arg1	study					259:263	the present study	247:263	the present study	247:263	In the present study, a major FnII protein has been identified and isolated from donkey (Equus hemionus) seminal plasma, which we refer to as Donkey Seminal Plasma protein-1 (DSP-1).
34863837	5	21	theme	Structural	833:842	arg1	studies					866:872	Structural and thermal stability studies	833:872	Structural and thermal stability studies on DSP-1 employing CD spectroscopy and differential scanning calorimetry	833:945	Structural and thermal stability studies on DSP-1 employing CD spectroscopy and differential scanning calorimetry showed that the protein unfolds at ~43 °C and binding to phosphorylcholine (PrC) - the head group moiety of choline phospholipids - increases its thermal stability.
34863837	2	22	theme	seminal	349:355	arg1	Equus					333:337	Equus	333:337	Equus	333:337	In the present study, a major FnII protein has been identified and isolated from donkey (Equus hemionus) seminal plasma, which we refer to as Donkey Seminal Plasma protein-1 (DSP-1).
34863837	2	22	theme	seminal	349:355	arg1	plasma					357:362	donkey (Equus hemionus) seminal plasma	325:362	donkey (Equus hemionus) seminal plasma	325:362	In the present study, a major FnII protein has been identified and isolated from donkey (Equus hemionus) seminal plasma, which we refer to as Donkey Seminal Plasma protein-1 (DSP-1).
34863837	0	23	theme	protein	106:112	arg1	DSP-1					114:118	the major donkey seminal plasma protein DSP-1	74:118	the major donkey seminal plasma protein DSP-1	74:118	Purification, molecular characterization and ligand binding properties of the major donkey seminal plasma protein DSP-1.
34863837	3	24	theme	mass	465:468	arg1	spectrometry					470:481	mass spectrometry	465:481	mass spectrometry	465:481	The amino acid sequence determined by mass spectrometry and computational modeling studies revealed that DSP-1 is homologous to other mammalian seminal plasma proteins, including bovine PDC-109 (also known as BSP-A1/A2) and equine HSP-1/2.
34863837	7	25	theme	DSP-1	1333:1337	arg1	binding					1339:1345	DSP-1 binding	1333:1345	DSP-1 binding	1333:1345	Further, interaction of DSP-1 with erythrocytes, a model cell membrane, revealed that DSP-1 binding is mediated by a specific interaction with choline phospholipids and results in membrane perturbation, suggesting that binding of this protein to sperm plasma membrane could be physiologically significant.
34863837	2	26	theme	Donkey	386:391	arg1	DSP-1					419:423	DSP-1	419:423	DSP-1	419:423	In the present study, a major FnII protein has been identified and isolated from donkey (Equus hemionus) seminal plasma, which we refer to as Donkey Seminal Plasma protein-1 (DSP-1).
34863837	2	26	theme	Donkey	386:391	arg1	protein-1					408:416	Donkey Seminal Plasma protein-1	386:416	Donkey Seminal Plasma protein-1 (DSP-1)	386:424	In the present study, a major FnII protein has been identified and isolated from donkey (Equus hemionus) seminal plasma, which we refer to as Donkey Seminal Plasma protein-1 (DSP-1).
34863837	3	27	theme	equine	651:656	arg1	HSP-1/2					658:664	equine HSP-1/2	651:664	equine HSP-1/2	651:664	The amino acid sequence determined by mass spectrometry and computational modeling studies revealed that DSP-1 is homologous to other mammalian seminal plasma proteins, including bovine PDC-109 (also known as BSP-A1/A2) and equine HSP-1/2.
34863837	4	28	theme	attached	815:822	arg1	glycans					824:830	the attached glycans	811:830	the attached glycans	811:830	High-resolution LC-MS analysis indicated that the protein is heterogeneously glycosylated and also contains multiple acetylations, occurring in the attached glycans.
34863837	4	29	theme	LC-MS	683:687	arg1	analysis					689:696	High-resolution LC-MS analysis	667:696	High-resolution LC-MS analysis	667:696	High-resolution LC-MS analysis indicated that the protein is heterogeneously glycosylated and also contains multiple acetylations, occurring in the attached glycans.
34863837	1	30	theme	major	172:176	arg1	proteins					178:185	the major proteins	168:185	the major proteins in many mammalian species including bull, horse and pig	168:241	Fibronectin type-II (FnII) family proteins are the major proteins in many mammalian species including bull, horse and pig.
34863837	1	30	theme	major	172:176	arg1	proteins					155:162	Fibronectin type-II (FnII) family proteins	121:162	Fibronectin type-II (FnII) family proteins	121:162	Fibronectin type-II (FnII) family proteins are the major proteins in many mammalian species including bull, horse and pig.
34863837	3	31	dep	known	627:631	arg1	also					622:625	also	622:625	also	622:625	The amino acid sequence determined by mass spectrometry and computational modeling studies revealed that DSP-1 is homologous to other mammalian seminal plasma proteins, including bovine PDC-109 (also known as BSP-A1/A2) and equine HSP-1/2.
34863837	7	32	theme	sperm	1493:1497	arg1	membrane					1506:1513	sperm plasma membrane	1493:1513	sperm plasma membrane	1493:1513	Further, interaction of DSP-1 with erythrocytes, a model cell membrane, revealed that DSP-1 binding is mediated by a specific interaction with choline phospholipids and results in membrane perturbation, suggesting that binding of this protein to sperm plasma membrane could be physiologically significant.
34863837	2	33	theme	FnII	274:277	arg1	protein					279:285	a major FnII protein	266:285	a major FnII protein	266:285	In the present study, a major FnII protein has been identified and isolated from donkey (Equus hemionus) seminal plasma, which we refer to as Donkey Seminal Plasma protein-1 (DSP-1).
34863837	4	34	theme	High-resolution	667:681	arg1	analysis					689:696	High-resolution LC-MS analysis	667:696	High-resolution LC-MS analysis	667:696	High-resolution LC-MS analysis indicated that the protein is heterogeneously glycosylated and also contains multiple acetylations, occurring in the attached glycans.
34863837	7	35	theme	specific	1364:1371	arg1	interaction					1373:1383	a specific interaction	1362:1383	a specific interaction with choline phospholipids and results in membrane perturbation	1362:1447	Further, interaction of DSP-1 with erythrocytes, a model cell membrane, revealed that DSP-1 binding is mediated by a specific interaction with choline phospholipids and results in membrane perturbation, suggesting that binding of this protein to sperm plasma membrane could be physiologically significant.
34863837	2	36	theme	major	268:272	arg1	protein					279:285	a major FnII protein	266:285	a major FnII protein	266:285	In the present study, a major FnII protein has been identified and isolated from donkey (Equus hemionus) seminal plasma, which we refer to as Donkey Seminal Plasma protein-1 (DSP-1).
34863837	7	37	theme	cell	1304:1307	arg1	membrane					1309:1316	a model cell membrane	1296:1316	a model cell membrane	1296:1316	Further, interaction of DSP-1 with erythrocytes, a model cell membrane, revealed that DSP-1 binding is mediated by a specific interaction with choline phospholipids and results in membrane perturbation, suggesting that binding of this protein to sperm plasma membrane could be physiologically significant.
34863837	7	37	theme	cell	1304:1307	arg1	erythrocytes					1282:1293	erythrocytes	1282:1293	erythrocytes	1282:1293	Further, interaction of DSP-1 with erythrocytes, a model cell membrane, revealed that DSP-1 binding is mediated by a specific interaction with choline phospholipids and results in membrane perturbation, suggesting that binding of this protein to sperm plasma membrane could be physiologically significant.
34863837	0	38	theme	molecular	14:22	arg1	characterization					24:39	molecular characterization	14:39	molecular characterization	14:39	Purification, molecular characterization and ligand binding properties of the major donkey seminal plasma protein DSP-1.
34863837	5	39	theme	differential	913:924	arg1	calorimetry					935:945	differential scanning calorimetry	913:945	differential scanning calorimetry	913:945	Structural and thermal stability studies on DSP-1 employing CD spectroscopy and differential scanning calorimetry showed that the protein unfolds at ~43 °C and binding to phosphorylcholine (PrC) - the head group moiety of choline phospholipids - increases its thermal stability.
34863837	5	40	theme	head	1034:1037	arg1	moiety					1045:1050	the head group moiety	1030:1050	the head group moiety of choline phospholipids	1030:1075	Structural and thermal stability studies on DSP-1 employing CD spectroscopy and differential scanning calorimetry showed that the protein unfolds at ~43 °C and binding to phosphorylcholine (PrC) - the head group moiety of choline phospholipids - increases its thermal stability.
34863837	3	41	theme	modeling	501:508	arg1	studies					510:516	computational modeling studies	487:516	computational modeling studies	487:516	The amino acid sequence determined by mass spectrometry and computational modeling studies revealed that DSP-1 is homologous to other mammalian seminal plasma proteins, including bovine PDC-109 (also known as BSP-A1/A2) and equine HSP-1/2.
34863837	1	42	theme	many	190:193	arg1	pig					239:241	pig	239:241	pig	239:241	Fibronectin type-II (FnII) family proteins are the major proteins in many mammalian species including bull, horse and pig.
34863837	1	42	theme	many	190:193	arg1	horse					229:233	horse	229:233	horse	229:233	Fibronectin type-II (FnII) family proteins are the major proteins in many mammalian species including bull, horse and pig.
34863837	1	42	theme	many	190:193	arg1	bull					223:226	bull	223:226	bull	223:226	Fibronectin type-II (FnII) family proteins are the major proteins in many mammalian species including bull, horse and pig.
34863837	1	42	theme	many	190:193	arg1	species					205:211	many mammalian species	190:211	many mammalian species including bull, horse and pig	190:241	Fibronectin type-II (FnII) family proteins are the major proteins in many mammalian species including bull, horse and pig.
34863837	0	43	theme	ligand	45:50	arg1	properties					60:69	ligand binding properties	45:69	ligand binding properties	45:69	Purification, molecular characterization and ligand binding properties of the major donkey seminal plasma protein DSP-1.
34863837	5	44	theme	group	1039:1043	arg1	moiety					1045:1050	the head group moiety	1030:1050	the head group moiety of choline phospholipids	1030:1075	Structural and thermal stability studies on DSP-1 employing CD spectroscopy and differential scanning calorimetry showed that the protein unfolds at ~43 °C and binding to phosphorylcholine (PrC) - the head group moiety of choline phospholipids - increases its thermal stability.
34863837	4	45	theme	multiple	775:782	arg1	acetylations					784:795	multiple acetylations	775:795	multiple acetylations	775:795	High-resolution LC-MS analysis indicated that the protein is heterogeneously glycosylated and also contains multiple acetylations, occurring in the attached glycans.
34863837	7	46	from	results	1416:1422	arg1	perturbation					1436:1447	membrane perturbation	1427:1447	membrane perturbation	1427:1447	Further, interaction of DSP-1 with erythrocytes, a model cell membrane, revealed that DSP-1 binding is mediated by a specific interaction with choline phospholipids and results in membrane perturbation, suggesting that binding of this protein to sperm plasma membrane could be physiologically significant.
34863837	1	47	theme	mammalian	195:203	arg1	pig					239:241	pig	239:241	pig	239:241	Fibronectin type-II (FnII) family proteins are the major proteins in many mammalian species including bull, horse and pig.
34863837	1	47	theme	mammalian	195:203	arg1	horse					229:233	horse	229:233	horse	229:233	Fibronectin type-II (FnII) family proteins are the major proteins in many mammalian species including bull, horse and pig.
34863837	1	47	theme	mammalian	195:203	arg1	bull					223:226	bull	223:226	bull	223:226	Fibronectin type-II (FnII) family proteins are the major proteins in many mammalian species including bull, horse and pig.
34863837	1	47	theme	mammalian	195:203	arg1	species					205:211	many mammalian species	190:211	many mammalian species including bull, horse and pig	190:241	Fibronectin type-II (FnII) family proteins are the major proteins in many mammalian species including bull, horse and pig.
34863837	4	48	gly	glycosylated	744:755	arg1	protein					717:723	the protein	713:723	the protein	713:723	High-resolution LC-MS analysis indicated that the protein is heterogeneously glycosylated and also contains multiple acetylations, occurring in the attached glycans.
34863837	7	49	theme	choline	1390:1396	arg1	phospholipids					1398:1410	choline phospholipids	1390:1410	choline phospholipids	1390:1410	Further, interaction of DSP-1 with erythrocytes, a model cell membrane, revealed that DSP-1 binding is mediated by a specific interaction with choline phospholipids and results in membrane perturbation, suggesting that binding of this protein to sperm plasma membrane could be physiologically significant.
34863837	3	50	theme	plasma	579:584	arg1	HSP-1/2					658:664	equine HSP-1/2	651:664	equine HSP-1/2	651:664	The amino acid sequence determined by mass spectrometry and computational modeling studies revealed that DSP-1 is homologous to other mammalian seminal plasma proteins, including bovine PDC-109 (also known as BSP-A1/A2) and equine HSP-1/2.
34863837	3	50	theme	plasma	579:584	arg1	proteins					586:593	other mammalian seminal plasma proteins	555:593	other mammalian seminal plasma proteins	555:593	The amino acid sequence determined by mass spectrometry and computational modeling studies revealed that DSP-1 is homologous to other mammalian seminal plasma proteins, including bovine PDC-109 (also known as BSP-A1/A2) and equine HSP-1/2.
34863837	3	50	theme	plasma	579:584	arg1	bovine					606:611	bovine PDC-109	606:619	bovine PDC-109 (also known as BSP-A1/A2)	606:645	The amino acid sequence determined by mass spectrometry and computational modeling studies revealed that DSP-1 is homologous to other mammalian seminal plasma proteins, including bovine PDC-109 (also known as BSP-A1/A2) and equine HSP-1/2.
34863837	7	51	theme	model	1298:1302	arg1	membrane					1309:1316	a model cell membrane	1296:1316	a model cell membrane	1296:1316	Further, interaction of DSP-1 with erythrocytes, a model cell membrane, revealed that DSP-1 binding is mediated by a specific interaction with choline phospholipids and results in membrane perturbation, suggesting that binding of this protein to sperm plasma membrane could be physiologically significant.
34863837	7	51	theme	model	1298:1302	arg1	erythrocytes					1282:1293	erythrocytes	1282:1293	erythrocytes	1282:1293	Further, interaction of DSP-1 with erythrocytes, a model cell membrane, revealed that DSP-1 binding is mediated by a specific interaction with choline phospholipids and results in membrane perturbation, suggesting that binding of this protein to sperm plasma membrane could be physiologically significant.
34863837	2	52	theme	Plasma	401:406	arg1	DSP-1					419:423	DSP-1	419:423	DSP-1	419:423	In the present study, a major FnII protein has been identified and isolated from donkey (Equus hemionus) seminal plasma, which we refer to as Donkey Seminal Plasma protein-1 (DSP-1).
34863837	2	52	theme	Plasma	401:406	arg1	protein-1					408:416	Donkey Seminal Plasma protein-1	386:416	Donkey Seminal Plasma protein-1 (DSP-1)	386:424	In the present study, a major FnII protein has been identified and isolated from donkey (Equus hemionus) seminal plasma, which we refer to as Donkey Seminal Plasma protein-1 (DSP-1).
34863837	5	53	theme	thermal	848:854	arg1	studies					866:872	Structural and thermal stability studies	833:872	Structural and thermal stability studies on DSP-1 employing CD spectroscopy and differential scanning calorimetry	833:945	Structural and thermal stability studies on DSP-1 employing CD spectroscopy and differential scanning calorimetry showed that the protein unfolds at ~43 °C and binding to phosphorylcholine (PrC) - the head group moiety of choline phospholipids - increases its thermal stability.
34863837	0	54	theme	binding	52:58	arg1	properties					60:69	ligand binding properties	45:69	ligand binding properties	45:69	Purification, molecular characterization and ligand binding properties of the major donkey seminal plasma protein DSP-1.
34863837	2	55	theme	Seminal	393:399	arg1	DSP-1					419:423	DSP-1	419:423	DSP-1	419:423	In the present study, a major FnII protein has been identified and isolated from donkey (Equus hemionus) seminal plasma, which we refer to as Donkey Seminal Plasma protein-1 (DSP-1).
34863837	2	55	theme	Seminal	393:399	arg1	protein-1					408:416	Donkey Seminal Plasma protein-1	386:416	Donkey Seminal Plasma protein-1 (DSP-1)	386:424	In the present study, a major FnII protein has been identified and isolated from donkey (Equus hemionus) seminal plasma, which we refer to as Donkey Seminal Plasma protein-1 (DSP-1).
34863837	6	56	theme	fluorescence	1122:1133	arg1	titrations					1135:1144	Intrinsic fluorescence titrations	1112:1144	Intrinsic fluorescence titrations	1112:1144	Intrinsic fluorescence titrations revealed that DSP-1 recognizes lyso-phosphatidylcholine with over 100-fold higher affinity than PrC.
34863837	1	57	theme	Fibronectin	121:131	arg1	proteins					178:185	the major proteins	168:185	the major proteins in many mammalian species including bull, horse and pig	168:241	Fibronectin type-II (FnII) family proteins are the major proteins in many mammalian species including bull, horse and pig.
34863837	1	57	theme	Fibronectin	121:131	arg1	proteins					155:162	Fibronectin type-II (FnII) family proteins	121:162	Fibronectin type-II (FnII) family proteins	121:162	Fibronectin type-II (FnII) family proteins are the major proteins in many mammalian species including bull, horse and pig.
34863837	7	58	theme	protein	1482:1488	arg1	binding					1466:1472	binding	1466:1472	binding of this protein to sperm plasma membrane	1466:1513	Further, interaction of DSP-1 with erythrocytes, a model cell membrane, revealed that DSP-1 binding is mediated by a specific interaction with choline phospholipids and results in membrane perturbation, suggesting that binding of this protein to sperm plasma membrane could be physiologically significant.
34863837	5	59	theme	scanning	926:933	arg1	calorimetry					935:945	differential scanning calorimetry	913:945	differential scanning calorimetry	913:945	Structural and thermal stability studies on DSP-1 employing CD spectroscopy and differential scanning calorimetry showed that the protein unfolds at ~43 °C and binding to phosphorylcholine (PrC) - the head group moiety of choline phospholipids - increases its thermal stability.
34863837	3	60	theme	mammalian	561:569	arg1	HSP-1/2					658:664	equine HSP-1/2	651:664	equine HSP-1/2	651:664	The amino acid sequence determined by mass spectrometry and computational modeling studies revealed that DSP-1 is homologous to other mammalian seminal plasma proteins, including bovine PDC-109 (also known as BSP-A1/A2) and equine HSP-1/2.
34863837	3	60	theme	mammalian	561:569	arg1	proteins					586:593	other mammalian seminal plasma proteins	555:593	other mammalian seminal plasma proteins	555:593	The amino acid sequence determined by mass spectrometry and computational modeling studies revealed that DSP-1 is homologous to other mammalian seminal plasma proteins, including bovine PDC-109 (also known as BSP-A1/A2) and equine HSP-1/2.
34863837	3	60	theme	mammalian	561:569	arg1	bovine					606:611	bovine PDC-109	606:619	bovine PDC-109 (also known as BSP-A1/A2)	606:645	The amino acid sequence determined by mass spectrometry and computational modeling studies revealed that DSP-1 is homologous to other mammalian seminal plasma proteins, including bovine PDC-109 (also known as BSP-A1/A2) and equine HSP-1/2.
34863837	1	61	theme	type-II	133:139	arg1	proteins					178:185	the major proteins	168:185	the major proteins in many mammalian species including bull, horse and pig	168:241	Fibronectin type-II (FnII) family proteins are the major proteins in many mammalian species including bull, horse and pig.
34863837	1	61	theme	type-II	133:139	arg1	proteins					155:162	Fibronectin type-II (FnII) family proteins	121:162	Fibronectin type-II (FnII) family proteins	121:162	Fibronectin type-II (FnII) family proteins are the major proteins in many mammalian species including bull, horse and pig.
34863837	3	62	theme	computational	487:499	arg1	studies					510:516	computational modeling studies	487:516	computational modeling studies	487:516	The amino acid sequence determined by mass spectrometry and computational modeling studies revealed that DSP-1 is homologous to other mammalian seminal plasma proteins, including bovine PDC-109 (also known as BSP-A1/A2) and equine HSP-1/2.
34863837	7	63	theme	membrane	1427:1434	arg1	perturbation					1436:1447	membrane perturbation	1427:1447	membrane perturbation	1427:1447	Further, interaction of DSP-1 with erythrocytes, a model cell membrane, revealed that DSP-1 binding is mediated by a specific interaction with choline phospholipids and results in membrane perturbation, suggesting that binding of this protein to sperm plasma membrane could be physiologically significant.
34863837	7	64	theme	plasma	1499:1504	arg1	membrane					1506:1513	sperm plasma membrane	1493:1513	sperm plasma membrane	1493:1513	Further, interaction of DSP-1 with erythrocytes, a model cell membrane, revealed that DSP-1 binding is mediated by a specific interaction with choline phospholipids and results in membrane perturbation, suggesting that binding of this protein to sperm plasma membrane could be physiologically significant.
34863837	2	65	dep	Equus	333:337	arg1	hemionus					339:346	Equus hemionus	333:346	Equus hemionus	333:346	In the present study, a major FnII protein has been identified and isolated from donkey (Equus hemionus) seminal plasma, which we refer to as Donkey Seminal Plasma protein-1 (DSP-1).
34863837	5	66	theme	stability	856:864	arg1	studies					866:872	Structural and thermal stability studies	833:872	Structural and thermal stability studies on DSP-1 employing CD spectroscopy and differential scanning calorimetry	833:945	Structural and thermal stability studies on DSP-1 employing CD spectroscopy and differential scanning calorimetry showed that the protein unfolds at ~43 °C and binding to phosphorylcholine (PrC) - the head group moiety of choline phospholipids - increases its thermal stability.
34863837	5	67	theme	CD	893:894	arg1	spectroscopy					896:907	CD spectroscopy	893:907	CD spectroscopy	893:907	Structural and thermal stability studies on DSP-1 employing CD spectroscopy and differential scanning calorimetry showed that the protein unfolds at ~43 °C and binding to phosphorylcholine (PrC) - the head group moiety of choline phospholipids - increases its thermal stability.
34863837	1	68	from	proteins	178:185	arg1	pig					239:241	pig	239:241	pig	239:241	Fibronectin type-II (FnII) family proteins are the major proteins in many mammalian species including bull, horse and pig.
34863837	1	68	from	proteins	178:185	arg1	horse					229:233	horse	229:233	horse	229:233	Fibronectin type-II (FnII) family proteins are the major proteins in many mammalian species including bull, horse and pig.
34863837	1	68	from	proteins	178:185	arg1	bull					223:226	bull	223:226	bull	223:226	Fibronectin type-II (FnII) family proteins are the major proteins in many mammalian species including bull, horse and pig.
34863837	1	68	from	proteins	178:185	arg1	species					205:211	many mammalian species	190:211	many mammalian species including bull, horse and pig	190:241	Fibronectin type-II (FnII) family proteins are the major proteins in many mammalian species including bull, horse and pig.
34863837	1	69	theme	FnII	142:145	arg1	proteins					178:185	the major proteins	168:185	the major proteins in many mammalian species including bull, horse and pig	168:241	Fibronectin type-II (FnII) family proteins are the major proteins in many mammalian species including bull, horse and pig.
34863837	1	69	theme	FnII	142:145	arg1	proteins					155:162	Fibronectin type-II (FnII) family proteins	121:162	Fibronectin type-II (FnII) family proteins	121:162	Fibronectin type-II (FnII) family proteins are the major proteins in many mammalian species including bull, horse and pig.
34863837	7	70	from	phospholipids	1398:1410	arg1	perturbation					1436:1447	membrane perturbation	1427:1447	membrane perturbation	1427:1447	Further, interaction of DSP-1 with erythrocytes, a model cell membrane, revealed that DSP-1 binding is mediated by a specific interaction with choline phospholipids and results in membrane perturbation, suggesting that binding of this protein to sperm plasma membrane could be physiologically significant.
34863837	3	71	theme	other	555:559	arg1	HSP-1/2					658:664	equine HSP-1/2	651:664	equine HSP-1/2	651:664	The amino acid sequence determined by mass spectrometry and computational modeling studies revealed that DSP-1 is homologous to other mammalian seminal plasma proteins, including bovine PDC-109 (also known as BSP-A1/A2) and equine HSP-1/2.
34863837	3	71	theme	other	555:559	arg1	proteins					586:593	other mammalian seminal plasma proteins	555:593	other mammalian seminal plasma proteins	555:593	The amino acid sequence determined by mass spectrometry and computational modeling studies revealed that DSP-1 is homologous to other mammalian seminal plasma proteins, including bovine PDC-109 (also known as BSP-A1/A2) and equine HSP-1/2.
34863837	3	71	theme	other	555:559	arg1	bovine					606:611	bovine PDC-109	606:619	bovine PDC-109 (also known as BSP-A1/A2)	606:645	The amino acid sequence determined by mass spectrometry and computational modeling studies revealed that DSP-1 is homologous to other mammalian seminal plasma proteins, including bovine PDC-109 (also known as BSP-A1/A2) and equine HSP-1/2.
34863837	7	72	theme	DSP-1	1271:1275	arg1	interaction					1256:1266	interaction	1256:1266	interaction of DSP-1 with erythrocytes, a model cell membrane,	1256:1317	Further, interaction of DSP-1 with erythrocytes, a model cell membrane, revealed that DSP-1 binding is mediated by a specific interaction with choline phospholipids and results in membrane perturbation, suggesting that binding of this protein to sperm plasma membrane could be physiologically significant.
34863837	2	73	theme	donkey	325:330	arg1	Equus					333:337	Equus	333:337	Equus	333:337	In the present study, a major FnII protein has been identified and isolated from donkey (Equus hemionus) seminal plasma, which we refer to as Donkey Seminal Plasma protein-1 (DSP-1).
34863837	2	73	theme	donkey	325:330	arg1	plasma					357:362	donkey (Equus hemionus) seminal plasma	325:362	donkey (Equus hemionus) seminal plasma	325:362	In the present study, a major FnII protein has been identified and isolated from donkey (Equus hemionus) seminal plasma, which we refer to as Donkey Seminal Plasma protein-1 (DSP-1).
33316214	8	0	from	children	1221:1228	arg1	samples					1204:1210	urine samples	1198:1210	urine samples from 415 children	1198:1228	FINDINGS Between July 1, 2017, and Dec 1, 2018, we obtained and stored urine samples from 415 children.
33316214	16	1	theme	tuberculosis	2390:2401	arg1	diagnosis					2377:2385	the rapid diagnosis	2367:2385	the rapid diagnosis of tuberculosis in children	2367:2413	FujiLAM could potentially add value to the rapid diagnosis of tuberculosis in children.
33316214	11	2	dep	CI	1503:1504	arg1	43·7-85·2					1506:1514	43·7-85·2	1506:1514	43·7-85·2	1506:1514	Using the microbiological reference standard, the sensitivity of FujiLAM was 64·9% (95% CI 43·7-85·2; positive in 40 of 63 confirmed samples) and the sensitivity of AlereLAM was 30·7% (8·6-61·6; 19 of 63).
33316214	15	3	with	comparison	2243:2252	arg1	FujiLAM					2269:2275	FujiLAM	2269:2275	FujiLAM	2269:2275	INTERPRETATION By comparison with AlereLAM, FujiLAM showed higher sensitivity and similar specificity.
33316214	2	4	theme	Fujifilm	378:385	arg1	FujiLAM					403:409	FujiLAM	403:409	FujiLAM	403:409	We assessed side by side the diagnostic accuracy of the urine-based lipoarabinomannan assays Fujifilm SILVAMP TB LAM (FujiLAM) and Alere Determine TB LAM Ag (AlereLAM) for detection of childhood tuberculosis.
33316214	2	4	theme	Fujifilm	378:385	arg1	LAM					398:400	Fujifilm SILVAMP TB LAM	378:400	the urine-based lipoarabinomannan assays Fujifilm SILVAMP TB LAM (FujiLAM)	337:410	We assessed side by side the diagnostic accuracy of the urine-based lipoarabinomannan assays Fujifilm SILVAMP TB LAM (FujiLAM) and Alere Determine TB LAM Ag (AlereLAM) for detection of childhood tuberculosis.
33316214	4	5	from	clinics	723:729	arg1	Nigeria					752:758	Nigeria	752:758	Nigeria	752:758	Children were consecutively recruited from four dedicated outpatient childhood tuberculosis clinics in The Gambia, Mali, Nigeria, and Tanzania.
33316214	4	5	from	clinics	723:729	arg1	Tanzania					765:772	Tanzania	765:772	Tanzania	765:772	Children were consecutively recruited from four dedicated outpatient childhood tuberculosis clinics in The Gambia, Mali, Nigeria, and Tanzania.
33316214	4	5	from	clinics	723:729	arg1	Mali					746:749	Mali	746:749	Mali	746:749	Children were consecutively recruited from four dedicated outpatient childhood tuberculosis clinics in The Gambia, Mali, Nigeria, and Tanzania.
33316214	4	5	from	clinics	723:729	arg1	Gambia					738:743	Gambia	738:743	Gambia	738:743	Children were consecutively recruited from four dedicated outpatient childhood tuberculosis clinics in The Gambia, Mali, Nigeria, and Tanzania.
33316214	1	6	theme	tuberculosis	271:282	arg1	diagnosis					248:256	the diagnosis	244:256	the diagnosis of childhood tuberculosis	244:282	BACKGROUND A sensitive and specific non-sputum-based test would be groundbreaking for the diagnosis of childhood tuberculosis.
33316214	6	7	theme	confirmed	938:946	arg1	tuberculosis					948:959	confirmed tuberculosis	938:959	confirmed tuberculosis	938:959	We measured diagnostic performance against a microbiological reference standard (confirmed tuberculosis) and a composite reference standard (confirmed and unconfirmed tuberculosis).
33316214	13	8	theme	standard	1833:1840	arg1	reference					1823:1831	the composite reference	1809:1831	the composite reference standard	1809:1840	Against the composite reference standard, both assays had decreased sensitivity; the sensitivity of FujiLAM was 32·9% (95% CI 24·6-41·9; positive in 58 of 176 confirmed and unconfirmed samples) and the sensitivity of AlereLAM was 20·2% (12·3-29·4; 36 of 176).
33316214	6	9	theme	standard	928:935	arg1	reference					918:926	a microbiological reference	900:926	a microbiological reference standard (confirmed tuberculosis)	900:960	We measured diagnostic performance against a microbiological reference standard (confirmed tuberculosis) and a composite reference standard (confirmed and unconfirmed tuberculosis).
33316214	14	10	from	negative	2117:2124	arg1	samples					2149:2155	202 of 239 unlikely samples	2129:2155	202 of 239 unlikely samples	2129:2155	The specificity of FujiLAM was 83·3% (95% CI 71·8-91·7; negative in 202 of 239 unlikely samples) and the specificity of AlereLAM was 90·0% (81·6-95·6; 216 of 239).
33316214	4	11	theme	dedicated	679:687	arg1	clinics					723:729	four dedicated outpatient childhood tuberculosis clinics	674:729	four dedicated outpatient childhood tuberculosis clinics in The Gambia, Mali, Nigeria, and Tanzania	674:772	Children were consecutively recruited from four dedicated outpatient childhood tuberculosis clinics in The Gambia, Mali, Nigeria, and Tanzania.
33316214	11	12	theme	AlereLAM	1580:1587	arg1	sensitivity					1565:1575	the sensitivity	1561:1575	the sensitivity of AlereLAM	1561:1587	Using the microbiological reference standard, the sensitivity of FujiLAM was 64·9% (95% CI 43·7-85·2; positive in 40 of 63 confirmed samples) and the sensitivity of AlereLAM was 30·7% (8·6-61·6; 19 of 63).
33316214	11	12	theme	AlereLAM	1580:1587	arg1	%					1597:1597	30·7%	1593:1597	30·7%	1593:1597	Using the microbiological reference standard, the sensitivity of FujiLAM was 64·9% (95% CI 43·7-85·2; positive in 40 of 63 confirmed samples) and the sensitivity of AlereLAM was 30·7% (8·6-61·6; 19 of 63).
33316214	14	13	theme	%	2101:2101	arg1	CI					2103:2104	95% CI 71·8-91·7	2099:2114	95% CI 71·8-91·7	2099:2114	The specificity of FujiLAM was 83·3% (95% CI 71·8-91·7; negative in 202 of 239 unlikely samples) and the specificity of AlereLAM was 90·0% (81·6-95·6; 216 of 239).
33316214	8	14	theme	415	1217:1219	arg1	children					1221:1228	415 children	1217:1228	415 children	1217:1228	FINDINGS Between July 1, 2017, and Dec 1, 2018, we obtained and stored urine samples from 415 children.
33316214	6	15	theme	microbiological	902:916	arg1	reference					918:926	a microbiological reference	900:926	a microbiological reference standard (confirmed tuberculosis)	900:960	We measured diagnostic performance against a microbiological reference standard (confirmed tuberculosis) and a composite reference standard (confirmed and unconfirmed tuberculosis).
33316214	4	16	theme	childhood	700:708	arg1	clinics					723:729	four dedicated outpatient childhood tuberculosis clinics	674:729	four dedicated outpatient childhood tuberculosis clinics in The Gambia, Mali, Nigeria, and Tanzania	674:772	Children were consecutively recruited from four dedicated outpatient childhood tuberculosis clinics in The Gambia, Mali, Nigeria, and Tanzania.
33316214	15	17	theme	similar	2307:2313	arg1	specificity					2315:2325	similar specificity	2307:2325	similar specificity	2307:2325	INTERPRETATION By comparison with AlereLAM, FujiLAM showed higher sensitivity and similar specificity.
33316214	1	18	theme	non-sputum-based	194:209	arg1	test					211:214	A sensitive and specific non-sputum-based test	169:214	A sensitive and specific non-sputum-based test	169:214	BACKGROUND A sensitive and specific non-sputum-based test would be groundbreaking for the diagnosis of childhood tuberculosis.
33316214	1	18	theme	non-sputum-based	194:209	arg1	groundbreaking					225:238	groundbreaking	225:238	groundbreaking	225:238	BACKGROUND A sensitive and specific non-sputum-based test would be groundbreaking for the diagnosis of childhood tuberculosis.
33316214	13	19	theme	%	1922:1922	arg1	CI					1924:1925	95% CI 24·6-41·9	1920:1935	95% CI 24·6-41·9	1920:1935	Against the composite reference standard, both assays had decreased sensitivity; the sensitivity of FujiLAM was 32·9% (95% CI 24·6-41·9; positive in 58 of 176 confirmed and unconfirmed samples) and the sensitivity of AlereLAM was 20·2% (12·3-29·4; 36 of 176).
33316214	3	20	theme	cross-sectional	510:524	arg1	study					526:530	this cross-sectional study	505:530	this cross-sectional study	505:530	METHODS In this cross-sectional study, we tested urine samples from children younger than 15 years with presumed pulmonary tuberculosis.
33316214	14	21	dep	81·6-95·6	2201:2209	arg1	239					2219:2221	239	2219:2221	239	2219:2221	The specificity of FujiLAM was 83·3% (95% CI 71·8-91·7; negative in 202 of 239 unlikely samples) and the specificity of AlereLAM was 90·0% (81·6-95·6; 216 of 239).
33316214	14	22	from	samples	2149:2155	arg1	negative					2117:2124	negative	2117:2124	negative	2117:2124	The specificity of FujiLAM was 83·3% (95% CI 71·8-91·7; negative in 202 of 239 unlikely samples) and the specificity of AlereLAM was 90·0% (81·6-95·6; 216 of 239).
33316214	12	23	dep	%	1774:1774	arg1	79·0-93·7					1777:1785	79·0-93·7	1777:1785	79·0-93·7	1777:1785	The specificity of FujiLAM was 83·8% (95% CI 76·5-89·4; negative in 297 of 352 unconfirmed and unlikely samples) and the specificity of AlereLAM was 87·8% (79·0-93·7; 312 of 352).
33316214	3	24	theme	presumed	598:605	arg1	tuberculosis					617:628	presumed pulmonary tuberculosis	598:628	presumed pulmonary tuberculosis	598:628	METHODS In this cross-sectional study, we tested urine samples from children younger than 15 years with presumed pulmonary tuberculosis.
33316214	6	25	dep	reference	978:986	arg1	confirmed					998:1006	confirmed	998:1006	confirmed	998:1006	We measured diagnostic performance against a microbiological reference standard (confirmed tuberculosis) and a composite reference standard (confirmed and unconfirmed tuberculosis).
33316214	6	25	dep	reference	978:986	arg1	unconfirmed					1012:1022	unconfirmed	1012:1022	unconfirmed tuberculosis	1012:1035	We measured diagnostic performance against a microbiological reference standard (confirmed tuberculosis) and a composite reference standard (confirmed and unconfirmed tuberculosis).
33316214	3	26	from	children	562:569	arg1	samples					549:555	urine samples	543:555	urine samples from children younger than 15 years with presumed pulmonary tuberculosis	543:628	METHODS In this cross-sectional study, we tested urine samples from children younger than 15 years with presumed pulmonary tuberculosis.
33316214	7	27	theme	bivariate	1087:1095	arg1	meta-analyses					1112:1124	bivariate random-effects meta-analyses	1087:1124	bivariate random-effects meta-analyses	1087:1124	Sensitivity and specificity were estimated with bivariate random-effects meta-analyses.
33316214	17	28	theme	Technology	2504:2513	arg1	Ministry					2439:2446	Ministry	2439:2446	Ministry	2439:2446	FUNDING German Federal Ministry of Education and Research, the Global Health Innovative Technology Fund, the UK Research and Innovation Global Challenges Research Fund, and the UK Medical Research Council.
33316214	17	28	theme	Technology	2504:2513	arg1	Fund					2515:2518	the Global Health Innovative Technology Fund	2475:2518	the Global Health Innovative Technology Fund	2475:2518	FUNDING German Federal Ministry of Education and Research, the Global Health Innovative Technology Fund, the UK Research and Innovation Global Challenges Research Fund, and the UK Medical Research Council.
33316214	11	29	theme	standard	1451:1458	arg1	reference					1441:1449	the microbiological reference	1421:1449	the microbiological reference standard	1421:1458	Using the microbiological reference standard, the sensitivity of FujiLAM was 64·9% (95% CI 43·7-85·2; positive in 40 of 63 confirmed samples) and the sensitivity of AlereLAM was 30·7% (8·6-61·6; 19 of 63).
33316214	5	30	theme	Biobanked	775:783	arg1	samples					791:797	Biobanked urine samples	775:797	Biobanked urine samples	775:797	Biobanked urine samples were thawed and tested using FujiLAM and AlereLAM assays.
33316214	0	31	theme	tuberculosis	79:90	arg1	diagnosis					56:64	diagnosis	56:64	diagnosis of pulmonary tuberculosis	56:90	Comparing accuracy of lipoarabinomannan urine tests for diagnosis of pulmonary tuberculosis in children from four African countries: a cross-sectional study.
33316214	13	32	theme	FujiLAM	1901:1907	arg1	%					1917:1917	32·9%	1913:1917	32·9%	1913:1917	Against the composite reference standard, both assays had decreased sensitivity; the sensitivity of FujiLAM was 32·9% (95% CI 24·6-41·9; positive in 58 of 176 confirmed and unconfirmed samples) and the sensitivity of AlereLAM was 20·2% (12·3-29·4; 36 of 176).
33316214	13	32	theme	FujiLAM	1901:1907	arg1	sensitivity					1886:1896	the sensitivity	1882:1896	the sensitivity of FujiLAM	1882:1907	Against the composite reference standard, both assays had decreased sensitivity; the sensitivity of FujiLAM was 32·9% (95% CI 24·6-41·9; positive in 58 of 176 confirmed and unconfirmed samples) and the sensitivity of AlereLAM was 20·2% (12·3-29·4; 36 of 176).
33316214	17	33	theme	UK	2525:2526	arg1	Research					2528:2535	UK Research	2525:2535	UK Research	2525:2535	FUNDING German Federal Ministry of Education and Research, the Global Health Innovative Technology Fund, the UK Research and Innovation Global Challenges Research Fund, and the UK Medical Research Council.
33316214	13	34	from	samples	1986:1992	arg1	positive					1938:1945	positive	1938:1945	positive	1938:1945	Against the composite reference standard, both assays had decreased sensitivity; the sensitivity of FujiLAM was 32·9% (95% CI 24·6-41·9; positive in 58 of 176 confirmed and unconfirmed samples) and the sensitivity of AlereLAM was 20·2% (12·3-29·4; 36 of 176).
33316214	3	35	with	years	587:591	arg1	tuberculosis					617:628	presumed pulmonary tuberculosis	598:628	presumed pulmonary tuberculosis	598:628	METHODS In this cross-sectional study, we tested urine samples from children younger than 15 years with presumed pulmonary tuberculosis.
33316214	9	36	theme	unconfirmed	1291:1301	arg1	tuberculosis					1303:1314	unconfirmed tuberculosis	1291:1314	unconfirmed tuberculosis	1291:1314	63 (15%) children had confirmed tuberculosis, 113 (27%) had unconfirmed tuberculosis, and 239 (58%) were unlikely to have tuberculosis.
33316214	17	37	theme	Medical	2596:2602	arg1	Council					2613:2619	the UK Medical Research Council	2589:2619	the UK Medical Research Council	2589:2619	FUNDING German Federal Ministry of Education and Research, the Global Health Innovative Technology Fund, the UK Research and Innovation Global Challenges Research Fund, and the UK Medical Research Council.
33316214	17	37	theme	Medical	2596:2602	arg1	Ministry					2439:2446	Ministry	2439:2446	Ministry	2439:2446	FUNDING German Federal Ministry of Education and Research, the Global Health Innovative Technology Fund, the UK Research and Innovation Global Challenges Research Fund, and the UK Medical Research Council.
33316214	14	38	dep	%	2198:2198	arg1	81·6-95·6					2201:2209	81·6-95·6	2201:2209	81·6-95·6	2201:2209	The specificity of FujiLAM was 83·3% (95% CI 71·8-91·7; negative in 202 of 239 unlikely samples) and the specificity of AlereLAM was 90·0% (81·6-95·6; 216 of 239).
33316214	1	39	dep	BACKGROUND	158:167	arg1	test					211:214	A sensitive and specific non-sputum-based test	169:214	A sensitive and specific non-sputum-based test	169:214	BACKGROUND A sensitive and specific non-sputum-based test would be groundbreaking for the diagnosis of childhood tuberculosis.
33316214	1	39	dep	BACKGROUND	158:167	arg1	groundbreaking					225:238	groundbreaking	225:238	groundbreaking	225:238	BACKGROUND A sensitive and specific non-sputum-based test would be groundbreaking for the diagnosis of childhood tuberculosis.
33316214	5	40	theme	FujiLAM	828:834	arg1	assays					849:854	FujiLAM and AlereLAM assays	828:854	FujiLAM and AlereLAM assays	828:854	Biobanked urine samples were thawed and tested using FujiLAM and AlereLAM assays.
33316214	16	41	from	diagnosis	2377:2385	arg1	children					2406:2413	children	2406:2413	children	2406:2413	FujiLAM could potentially add value to the rapid diagnosis of tuberculosis in children.
33316214	2	42	dep	assays	371:376	arg1	FujiLAM					403:409	FujiLAM	403:409	FujiLAM	403:409	We assessed side by side the diagnostic accuracy of the urine-based lipoarabinomannan assays Fujifilm SILVAMP TB LAM (FujiLAM) and Alere Determine TB LAM Ag (AlereLAM) for detection of childhood tuberculosis.
33316214	2	42	dep	assays	371:376	arg1	LAM					398:400	Fujifilm SILVAMP TB LAM	378:400	the urine-based lipoarabinomannan assays Fujifilm SILVAMP TB LAM (FujiLAM)	337:410	We assessed side by side the diagnostic accuracy of the urine-based lipoarabinomannan assays Fujifilm SILVAMP TB LAM (FujiLAM) and Alere Determine TB LAM Ag (AlereLAM) for detection of childhood tuberculosis.
33316214	11	43	theme	microbiological	1425:1439	arg1	reference					1441:1449	the microbiological reference	1421:1449	the microbiological reference standard	1421:1458	Using the microbiological reference standard, the sensitivity of FujiLAM was 64·9% (95% CI 43·7-85·2; positive in 40 of 63 confirmed samples) and the sensitivity of AlereLAM was 30·7% (8·6-61·6; 19 of 63).
33316214	2	44	theme	LAM	435:437	arg1	Ag					439:440	TB LAM Ag	432:440	TB LAM Ag	432:440	We assessed side by side the diagnostic accuracy of the urine-based lipoarabinomannan assays Fujifilm SILVAMP TB LAM (FujiLAM) and Alere Determine TB LAM Ag (AlereLAM) for detection of childhood tuberculosis.
33316214	5	45	theme	AlereLAM	840:847	arg1	assays					849:854	FujiLAM and AlereLAM assays	828:854	FujiLAM and AlereLAM assays	828:854	Biobanked urine samples were thawed and tested using FujiLAM and AlereLAM assays.
33316214	13	46	theme	AlereLAM	2018:2025	arg1	%					2035:2035	20·2%	2031:2035	20·2%	2031:2035	Against the composite reference standard, both assays had decreased sensitivity; the sensitivity of FujiLAM was 32·9% (95% CI 24·6-41·9; positive in 58 of 176 confirmed and unconfirmed samples) and the sensitivity of AlereLAM was 20·2% (12·3-29·4; 36 of 176).
33316214	13	46	theme	AlereLAM	2018:2025	arg1	sensitivity					2003:2013	the sensitivity	1999:2013	the sensitivity of AlereLAM	1999:2025	Against the composite reference standard, both assays had decreased sensitivity; the sensitivity of FujiLAM was 32·9% (95% CI 24·6-41·9; positive in 58 of 176 confirmed and unconfirmed samples) and the sensitivity of AlereLAM was 20·2% (12·3-29·4; 36 of 176).
33316214	12	47	theme	unlikely	1716:1723	arg1	samples					1725:1731	297 of 352 unconfirmed and unlikely samples	1689:1731	297 of 352 unconfirmed and unlikely samples	1689:1731	The specificity of FujiLAM was 83·8% (95% CI 76·5-89·4; negative in 297 of 352 unconfirmed and unlikely samples) and the specificity of AlereLAM was 87·8% (79·0-93·7; 312 of 352).
33316214	2	48	theme	childhood	470:478	arg1	tuberculosis					480:491	childhood tuberculosis	470:491	childhood tuberculosis	470:491	We assessed side by side the diagnostic accuracy of the urine-based lipoarabinomannan assays Fujifilm SILVAMP TB LAM (FujiLAM) and Alere Determine TB LAM Ag (AlereLAM) for detection of childhood tuberculosis.
33316214	13	49	from	positive	1938:1945	arg1	samples					1986:1992	58 of 176 confirmed and unconfirmed samples	1950:1992	58 of 176 confirmed and unconfirmed samples	1950:1992	Against the composite reference standard, both assays had decreased sensitivity; the sensitivity of FujiLAM was 32·9% (95% CI 24·6-41·9; positive in 58 of 176 confirmed and unconfirmed samples) and the sensitivity of AlereLAM was 20·2% (12·3-29·4; 36 of 176).
33316214	0	50	theme	urine	40:44	arg1	tests					46:50	lipoarabinomannan urine tests	22:50	lipoarabinomannan urine tests	22:50	Comparing accuracy of lipoarabinomannan urine tests for diagnosis of pulmonary tuberculosis in children from four African countries: a cross-sectional study.
33316214	17	51	theme	Health	2486:2491	arg1	Ministry					2439:2446	Ministry	2439:2446	Ministry	2439:2446	FUNDING German Federal Ministry of Education and Research, the Global Health Innovative Technology Fund, the UK Research and Innovation Global Challenges Research Fund, and the UK Medical Research Council.
33316214	17	51	theme	Health	2486:2491	arg1	Fund					2515:2518	the Global Health Innovative Technology Fund	2475:2518	the Global Health Innovative Technology Fund	2475:2518	FUNDING German Federal Ministry of Education and Research, the Global Health Innovative Technology Fund, the UK Research and Innovation Global Challenges Research Fund, and the UK Medical Research Council.
33316214	2	52	theme	assays	371:376	arg1	accuracy					325:332	the diagnostic accuracy	310:332	the diagnostic accuracy of the urine-based lipoarabinomannan assays Fujifilm SILVAMP TB LAM (FujiLAM)	310:410	We assessed side by side the diagnostic accuracy of the urine-based lipoarabinomannan assays Fujifilm SILVAMP TB LAM (FujiLAM) and Alere Determine TB LAM Ag (AlereLAM) for detection of childhood tuberculosis.
33316214	14	53	theme	unlikely	2140:2147	arg1	samples					2149:2155	202 of 239 unlikely samples	2129:2155	202 of 239 unlikely samples	2129:2155	The specificity of FujiLAM was 83·3% (95% CI 71·8-91·7; negative in 202 of 239 unlikely samples) and the specificity of AlereLAM was 90·0% (81·6-95·6; 216 of 239).
33316214	2	54	theme	urine-based	341:351	arg1	assays					371:376	the urine-based lipoarabinomannan assays Fujifilm SILVAMP TB LAM (FujiLAM)	337:410	the urine-based lipoarabinomannan assays Fujifilm SILVAMP TB LAM (FujiLAM)	337:410	We assessed side by side the diagnostic accuracy of the urine-based lipoarabinomannan assays Fujifilm SILVAMP TB LAM (FujiLAM) and Alere Determine TB LAM Ag (AlereLAM) for detection of childhood tuberculosis.
33316214	15	55	theme	higher	2284:2289	arg1	sensitivity					2291:2301	higher sensitivity	2284:2301	higher sensitivity	2284:2301	INTERPRETATION By comparison with AlereLAM, FujiLAM showed higher sensitivity and similar specificity.
33316214	12	56	theme	unconfirmed	1700:1710	arg1	samples					1725:1731	297 of 352 unconfirmed and unlikely samples	1689:1731	297 of 352 unconfirmed and unlikely samples	1689:1731	The specificity of FujiLAM was 83·8% (95% CI 76·5-89·4; negative in 297 of 352 unconfirmed and unlikely samples) and the specificity of AlereLAM was 87·8% (79·0-93·7; 312 of 352).
33316214	12	57	theme	%	1661:1661	arg1	CI					1663:1664	95% CI 76·5-89·4	1659:1674	95% CI 76·5-89·4	1659:1674	The specificity of FujiLAM was 83·8% (95% CI 76·5-89·4; negative in 297 of 352 unconfirmed and unlikely samples) and the specificity of AlereLAM was 87·8% (79·0-93·7; 312 of 352).
33316214	13	58	theme	composite	1813:1821	arg1	reference					1823:1831	the composite reference	1809:1831	the composite reference standard	1809:1840	Against the composite reference standard, both assays had decreased sensitivity; the sensitivity of FujiLAM was 32·9% (95% CI 24·6-41·9; positive in 58 of 176 confirmed and unconfirmed samples) and the sensitivity of AlereLAM was 20·2% (12·3-29·4; 36 of 176).
33316214	10	59	dep	HIV-positive	1384:1395	arg1	%					1411:1411	prevalence 15%	1398:1411	prevalence 15%	1398:1411	61 children were HIV-positive (prevalence 15%).
33316214	0	60	dep	Comparing	0:8	arg1	study					151:155	a cross-sectional study	133:155	a cross-sectional study	133:155	Comparing accuracy of lipoarabinomannan urine tests for diagnosis of pulmonary tuberculosis in children from four African countries: a cross-sectional study.
33316214	16	61	theme	rapid	2371:2375	arg1	diagnosis					2377:2385	the rapid diagnosis	2367:2385	the rapid diagnosis of tuberculosis in children	2367:2413	FujiLAM could potentially add value to the rapid diagnosis of tuberculosis in children.
33316214	6	62	dep	standard	928:935	arg1	tuberculosis					948:959	confirmed tuberculosis	938:959	confirmed tuberculosis	938:959	We measured diagnostic performance against a microbiological reference standard (confirmed tuberculosis) and a composite reference standard (confirmed and unconfirmed tuberculosis).
33316214	11	63	theme	confirmed	1538:1546	arg1	samples					1548:1554	40 of 63 confirmed samples	1529:1554	40 of 63 confirmed samples	1529:1554	Using the microbiological reference standard, the sensitivity of FujiLAM was 64·9% (95% CI 43·7-85·2; positive in 40 of 63 confirmed samples) and the sensitivity of AlereLAM was 30·7% (8·6-61·6; 19 of 63).
33316214	2	64	theme	SILVAMP	387:393	arg1	FujiLAM					403:409	FujiLAM	403:409	FujiLAM	403:409	We assessed side by side the diagnostic accuracy of the urine-based lipoarabinomannan assays Fujifilm SILVAMP TB LAM (FujiLAM) and Alere Determine TB LAM Ag (AlereLAM) for detection of childhood tuberculosis.
33316214	2	64	theme	SILVAMP	387:393	arg1	LAM					398:400	Fujifilm SILVAMP TB LAM	378:400	the urine-based lipoarabinomannan assays Fujifilm SILVAMP TB LAM (FujiLAM)	337:410	We assessed side by side the diagnostic accuracy of the urine-based lipoarabinomannan assays Fujifilm SILVAMP TB LAM (FujiLAM) and Alere Determine TB LAM Ag (AlereLAM) for detection of childhood tuberculosis.
33316214	1	65	theme	childhood	261:269	arg1	tuberculosis					271:282	childhood tuberculosis	261:282	childhood tuberculosis	261:282	BACKGROUND A sensitive and specific non-sputum-based test would be groundbreaking for the diagnosis of childhood tuberculosis.
33316214	17	66	theme	UK	2593:2594	arg1	Council					2613:2619	the UK Medical Research Council	2589:2619	the UK Medical Research Council	2589:2619	FUNDING German Federal Ministry of Education and Research, the Global Health Innovative Technology Fund, the UK Research and Innovation Global Challenges Research Fund, and the UK Medical Research Council.
33316214	17	66	theme	UK	2593:2594	arg1	Ministry					2439:2446	Ministry	2439:2446	Ministry	2439:2446	FUNDING German Federal Ministry of Education and Research, the Global Health Innovative Technology Fund, the UK Research and Innovation Global Challenges Research Fund, and the UK Medical Research Council.
33316214	3	67	theme	urine	543:547	arg1	samples					549:555	urine samples	543:555	urine samples from children younger than 15 years with presumed pulmonary tuberculosis	543:628	METHODS In this cross-sectional study, we tested urine samples from children younger than 15 years with presumed pulmonary tuberculosis.
33316214	14	68	theme	AlereLAM	2181:2188	arg1	%					2198:2198	90·0%	2194:2198	90·0%	2194:2198	The specificity of FujiLAM was 83·3% (95% CI 71·8-91·7; negative in 202 of 239 unlikely samples) and the specificity of AlereLAM was 90·0% (81·6-95·6; 216 of 239).
33316214	14	68	theme	AlereLAM	2181:2188	arg1	specificity					2166:2176	the specificity	2162:2176	the specificity of AlereLAM	2162:2188	The specificity of FujiLAM was 83·3% (95% CI 71·8-91·7; negative in 202 of 239 unlikely samples) and the specificity of AlereLAM was 90·0% (81·6-95·6; 216 of 239).
33316214	11	69	dep	positive	1517:1524	arg1	%					1501:1501	95%	1499:1501	95% CI 43·7-85·2	1499:1514	Using the microbiological reference standard, the sensitivity of FujiLAM was 64·9% (95% CI 43·7-85·2; positive in 40 of 63 confirmed samples) and the sensitivity of AlereLAM was 30·7% (8·6-61·6; 19 of 63).
33316214	17	70	theme	Global	2552:2557	arg1	Challenges					2559:2568	Innovation Global Challenges	2541:2568	Innovation Global Challenges	2541:2568	FUNDING German Federal Ministry of Education and Research, the Global Health Innovative Technology Fund, the UK Research and Innovation Global Challenges Research Fund, and the UK Medical Research Council.
33316214	9	71	contain	have	1348:1351	arg1	239					1321:1323	239	1321:1323	239	1321:1323	63 (15%) children had confirmed tuberculosis, 113 (27%) had unconfirmed tuberculosis, and 239 (58%) were unlikely to have tuberculosis.
33316214	9	71	contain	have	1348:1351	arg1	%					1328:1328	58%	1326:1328	58%	1326:1328	63 (15%) children had confirmed tuberculosis, 113 (27%) had unconfirmed tuberculosis, and 239 (58%) were unlikely to have tuberculosis.
33316214	9	71	contain	have	1348:1351	arg2	tuberculosis					1353:1364	tuberculosis	1353:1364	tuberculosis	1353:1364	63 (15%) children had confirmed tuberculosis, 113 (27%) had unconfirmed tuberculosis, and 239 (58%) were unlikely to have tuberculosis.
33316214	2	72	theme	side	297:300	arg1	accuracy					325:332	the diagnostic accuracy	310:332	the diagnostic accuracy of the urine-based lipoarabinomannan assays Fujifilm SILVAMP TB LAM (FujiLAM)	310:410	We assessed side by side the diagnostic accuracy of the urine-based lipoarabinomannan assays Fujifilm SILVAMP TB LAM (FujiLAM) and Alere Determine TB LAM Ag (AlereLAM) for detection of childhood tuberculosis.
33316214	1	73	theme	specific	185:192	arg1	test					211:214	A sensitive and specific non-sputum-based test	169:214	A sensitive and specific non-sputum-based test	169:214	BACKGROUND A sensitive and specific non-sputum-based test would be groundbreaking for the diagnosis of childhood tuberculosis.
33316214	1	73	theme	specific	185:192	arg1	groundbreaking					225:238	groundbreaking	225:238	groundbreaking	225:238	BACKGROUND A sensitive and specific non-sputum-based test would be groundbreaking for the diagnosis of childhood tuberculosis.
33316214	1	74	theme	sensitive	171:179	arg1	test					211:214	A sensitive and specific non-sputum-based test	169:214	A sensitive and specific non-sputum-based test	169:214	BACKGROUND A sensitive and specific non-sputum-based test would be groundbreaking for the diagnosis of childhood tuberculosis.
33316214	1	74	theme	sensitive	171:179	arg1	groundbreaking					225:238	groundbreaking	225:238	groundbreaking	225:238	BACKGROUND A sensitive and specific non-sputum-based test would be groundbreaking for the diagnosis of childhood tuberculosis.
33316214	8	75	theme	urine	1198:1202	arg1	samples					1204:1210	urine samples	1198:1210	urine samples from 415 children	1198:1228	FINDINGS Between July 1, 2017, and Dec 1, 2018, we obtained and stored urine samples from 415 children.
33316214	3	76	theme	younger	571:577	arg1	children					562:569	children	562:569	children younger than 15 years with presumed pulmonary tuberculosis	562:628	METHODS In this cross-sectional study, we tested urine samples from children younger than 15 years with presumed pulmonary tuberculosis.
33316214	11	77	from	samples	1548:1554	arg1	positive					1517:1524	positive	1517:1524	positive	1517:1524	Using the microbiological reference standard, the sensitivity of FujiLAM was 64·9% (95% CI 43·7-85·2; positive in 40 of 63 confirmed samples) and the sensitivity of AlereLAM was 30·7% (8·6-61·6; 19 of 63).
33316214	12	78	theme	FujiLAM	1640:1646	arg1	%					1656:1656	83·8%	1652:1656	83·8%	1652:1656	The specificity of FujiLAM was 83·8% (95% CI 76·5-89·4; negative in 297 of 352 unconfirmed and unlikely samples) and the specificity of AlereLAM was 87·8% (79·0-93·7; 312 of 352).
33316214	12	78	theme	FujiLAM	1640:1646	arg1	specificity					1625:1635	The specificity	1621:1635	The specificity of FujiLAM	1621:1646	The specificity of FujiLAM was 83·8% (95% CI 76·5-89·4; negative in 297 of 352 unconfirmed and unlikely samples) and the specificity of AlereLAM was 87·8% (79·0-93·7; 312 of 352).
33316214	4	79	theme	outpatient	689:698	arg1	clinics					723:729	four dedicated outpatient childhood tuberculosis clinics	674:729	four dedicated outpatient childhood tuberculosis clinics in The Gambia, Mali, Nigeria, and Tanzania	674:772	Children were consecutively recruited from four dedicated outpatient childhood tuberculosis clinics in The Gambia, Mali, Nigeria, and Tanzania.
33316214	11	80	theme	CI	1503:1504	arg1	%					1501:1501	95%	1499:1501	95% CI 43·7-85·2	1499:1514	Using the microbiological reference standard, the sensitivity of FujiLAM was 64·9% (95% CI 43·7-85·2; positive in 40 of 63 confirmed samples) and the sensitivity of AlereLAM was 30·7% (8·6-61·6; 19 of 63).
33316214	2	81	theme	diagnostic	314:323	arg1	accuracy					325:332	the diagnostic accuracy	310:332	the diagnostic accuracy of the urine-based lipoarabinomannan assays Fujifilm SILVAMP TB LAM (FujiLAM)	310:410	We assessed side by side the diagnostic accuracy of the urine-based lipoarabinomannan assays Fujifilm SILVAMP TB LAM (FujiLAM) and Alere Determine TB LAM Ag (AlereLAM) for detection of childhood tuberculosis.
33316214	11	82	from	positive	1517:1524	arg1	samples					1548:1554	40 of 63 confirmed samples	1529:1554	40 of 63 confirmed samples	1529:1554	Using the microbiological reference standard, the sensitivity of FujiLAM was 64·9% (95% CI 43·7-85·2; positive in 40 of 63 confirmed samples) and the sensitivity of AlereLAM was 30·7% (8·6-61·6; 19 of 63).
33316214	12	83	from	samples	1725:1731	arg1	negative					1677:1684	negative	1677:1684	negative	1677:1684	The specificity of FujiLAM was 83·8% (95% CI 76·5-89·4; negative in 297 of 352 unconfirmed and unlikely samples) and the specificity of AlereLAM was 87·8% (79·0-93·7; 312 of 352).
33316214	17	84	theme	Challenges	2559:2568	arg1	Fund					2579:2582	the UK Research and Innovation Global Challenges Research Fund	2521:2582	the UK Research and Innovation Global Challenges Research Fund	2521:2582	FUNDING German Federal Ministry of Education and Research, the Global Health Innovative Technology Fund, the UK Research and Innovation Global Challenges Research Fund, and the UK Medical Research Council.
33316214	17	84	theme	Challenges	2559:2568	arg1	Ministry					2439:2446	Ministry	2439:2446	Ministry	2439:2446	FUNDING German Federal Ministry of Education and Research, the Global Health Innovative Technology Fund, the UK Research and Innovation Global Challenges Research Fund, and the UK Medical Research Council.
33316214	0	85	theme	cross-sectional	135:149	arg1	study					151:155	a cross-sectional study	133:155	a cross-sectional study	133:155	Comparing accuracy of lipoarabinomannan urine tests for diagnosis of pulmonary tuberculosis in children from four African countries: a cross-sectional study.
33316214	6	86	theme	standard	988:995	arg1	reference					978:986	a composite reference standard	966:995	a composite reference standard (confirmed and unconfirmed tuberculosis)	966:1036	We measured diagnostic performance against a microbiological reference standard (confirmed tuberculosis) and a composite reference standard (confirmed and unconfirmed tuberculosis).
33316214	7	87	theme	random-effects	1097:1110	arg1	meta-analyses					1112:1124	bivariate random-effects meta-analyses	1087:1124	bivariate random-effects meta-analyses	1087:1124	Sensitivity and specificity were estimated with bivariate random-effects meta-analyses.
33316214	6	88	theme	composite	968:976	arg1	reference					978:986	a composite reference standard	966:995	a composite reference standard (confirmed and unconfirmed tuberculosis)	966:1036	We measured diagnostic performance against a microbiological reference standard (confirmed tuberculosis) and a composite reference standard (confirmed and unconfirmed tuberculosis).
33316214	13	89	dep	positive	1938:1945	arg1	CI					1924:1925	95% CI 24·6-41·9	1920:1935	95% CI 24·6-41·9	1920:1935	Against the composite reference standard, both assays had decreased sensitivity; the sensitivity of FujiLAM was 32·9% (95% CI 24·6-41·9; positive in 58 of 176 confirmed and unconfirmed samples) and the sensitivity of AlereLAM was 20·2% (12·3-29·4; 36 of 176).
33316214	14	90	theme	FujiLAM	2080:2086	arg1	%					2096:2096	83·3%	2092:2096	83·3%	2092:2096	The specificity of FujiLAM was 83·3% (95% CI 71·8-91·7; negative in 202 of 239 unlikely samples) and the specificity of AlereLAM was 90·0% (81·6-95·6; 216 of 239).
33316214	14	90	theme	FujiLAM	2080:2086	arg1	specificity					2065:2075	The specificity	2061:2075	The specificity of FujiLAM	2061:2086	The specificity of FujiLAM was 83·3% (95% CI 71·8-91·7; negative in 202 of 239 unlikely samples) and the specificity of AlereLAM was 90·0% (81·6-95·6; 216 of 239).
33316214	17	91	theme	Research	2570:2577	arg1	Fund					2579:2582	the UK Research and Innovation Global Challenges Research Fund	2521:2582	the UK Research and Innovation Global Challenges Research Fund	2521:2582	FUNDING German Federal Ministry of Education and Research, the Global Health Innovative Technology Fund, the UK Research and Innovation Global Challenges Research Fund, and the UK Medical Research Council.
33316214	17	91	theme	Research	2570:2577	arg1	Ministry					2439:2446	Ministry	2439:2446	Ministry	2439:2446	FUNDING German Federal Ministry of Education and Research, the Global Health Innovative Technology Fund, the UK Research and Innovation Global Challenges Research Fund, and the UK Medical Research Council.
33316214	0	92	theme	pulmonary	69:77	arg1	tuberculosis					79:90	pulmonary tuberculosis	69:90	pulmonary tuberculosis	69:90	Comparing accuracy of lipoarabinomannan urine tests for diagnosis of pulmonary tuberculosis in children from four African countries: a cross-sectional study.
33316214	3	93	theme	pulmonary	607:615	arg1	tuberculosis					617:628	presumed pulmonary tuberculosis	598:628	presumed pulmonary tuberculosis	598:628	METHODS In this cross-sectional study, we tested urine samples from children younger than 15 years with presumed pulmonary tuberculosis.
33316214	4	94	theme	tuberculosis	710:721	arg1	clinics					723:729	four dedicated outpatient childhood tuberculosis clinics	674:729	four dedicated outpatient childhood tuberculosis clinics in The Gambia, Mali, Nigeria, and Tanzania	674:772	Children were consecutively recruited from four dedicated outpatient childhood tuberculosis clinics in The Gambia, Mali, Nigeria, and Tanzania.
33316214	11	95	theme	FujiLAM	1480:1486	arg1	%					1496:1496	64·9%	1492:1496	64·9%	1492:1496	Using the microbiological reference standard, the sensitivity of FujiLAM was 64·9% (95% CI 43·7-85·2; positive in 40 of 63 confirmed samples) and the sensitivity of AlereLAM was 30·7% (8·6-61·6; 19 of 63).
33316214	11	95	theme	FujiLAM	1480:1486	arg1	sensitivity					1465:1475	the sensitivity	1461:1475	the sensitivity of FujiLAM	1461:1486	Using the microbiological reference standard, the sensitivity of FujiLAM was 64·9% (95% CI 43·7-85·2; positive in 40 of 63 confirmed samples) and the sensitivity of AlereLAM was 30·7% (8·6-61·6; 19 of 63).
33316214	5	96	theme	urine	785:789	arg1	samples					791:797	Biobanked urine samples	775:797	Biobanked urine samples	775:797	Biobanked urine samples were thawed and tested using FujiLAM and AlereLAM assays.
33316214	13	97	theme	decreased	1859:1867	arg1	sensitivity					1869:1879	decreased sensitivity	1859:1879	decreased sensitivity	1859:1879	Against the composite reference standard, both assays had decreased sensitivity; the sensitivity of FujiLAM was 32·9% (95% CI 24·6-41·9; positive in 58 of 176 confirmed and unconfirmed samples) and the sensitivity of AlereLAM was 20·2% (12·3-29·4; 36 of 176).
33316214	2	98	theme	tuberculosis	480:491	arg1	detection					457:465	detection	457:465	detection of childhood tuberculosis	457:491	We assessed side by side the diagnostic accuracy of the urine-based lipoarabinomannan assays Fujifilm SILVAMP TB LAM (FujiLAM) and Alere Determine TB LAM Ag (AlereLAM) for detection of childhood tuberculosis.
33316214	12	99	dep	79·0-93·7	1777:1785	arg1	352					1795:1797	352	1795:1797	352	1795:1797	The specificity of FujiLAM was 83·8% (95% CI 76·5-89·4; negative in 297 of 352 unconfirmed and unlikely samples) and the specificity of AlereLAM was 87·8% (79·0-93·7; 312 of 352).
33316214	17	100	theme	Research	2528:2535	arg1	Fund					2579:2582	the UK Research and Innovation Global Challenges Research Fund	2521:2582	the UK Research and Innovation Global Challenges Research Fund	2521:2582	FUNDING German Federal Ministry of Education and Research, the Global Health Innovative Technology Fund, the UK Research and Innovation Global Challenges Research Fund, and the UK Medical Research Council.
33316214	17	100	theme	Research	2528:2535	arg1	Ministry					2439:2446	Ministry	2439:2446	Ministry	2439:2446	FUNDING German Federal Ministry of Education and Research, the Global Health Innovative Technology Fund, the UK Research and Innovation Global Challenges Research Fund, and the UK Medical Research Council.
33316214	10	101	theme	prevalence	1398:1407	arg1	%					1411:1411	prevalence 15%	1398:1411	prevalence 15%	1398:1411	61 children were HIV-positive (prevalence 15%).
33316214	9	102	contain	had	1287:1289	arg2	tuberculosis					1303:1314	unconfirmed tuberculosis	1291:1314	unconfirmed tuberculosis	1291:1314	63 (15%) children had confirmed tuberculosis, 113 (27%) had unconfirmed tuberculosis, and 239 (58%) were unlikely to have tuberculosis.
33316214	9	102	contain	had	1287:1289	arg1	113					1277:1279	113	1277:1279	113	1277:1279	63 (15%) children had confirmed tuberculosis, 113 (27%) had unconfirmed tuberculosis, and 239 (58%) were unlikely to have tuberculosis.
33316214	9	102	contain	had	1287:1289	arg1	%					1284:1284	27%	1282:1284	27%	1282:1284	63 (15%) children had confirmed tuberculosis, 113 (27%) had unconfirmed tuberculosis, and 239 (58%) were unlikely to have tuberculosis.
33316214	0	103	theme	African	114:120	arg1	countries					122:130	four African countries	109:130	four African countries	109:130	Comparing accuracy of lipoarabinomannan urine tests for diagnosis of pulmonary tuberculosis in children from four African countries: a cross-sectional study.
33316214	3	104	dep	METHODS	494:500	arg1	tested					536:541	tested	536:541	tested urine samples from children younger than 15 years with presumed pulmonary tuberculosis	536:628	METHODS In this cross-sectional study, we tested urine samples from children younger than 15 years with presumed pulmonary tuberculosis.
33316214	17	105	theme	Innovation	2541:2550	arg1	Challenges					2559:2568	Innovation Global Challenges	2541:2568	Innovation Global Challenges	2541:2568	FUNDING German Federal Ministry of Education and Research, the Global Health Innovative Technology Fund, the UK Research and Innovation Global Challenges Research Fund, and the UK Medical Research Council.
33316214	13	106	theme	confirmed	1960:1968	arg1	samples					1986:1992	58 of 176 confirmed and unconfirmed samples	1950:1992	58 of 176 confirmed and unconfirmed samples	1950:1992	Against the composite reference standard, both assays had decreased sensitivity; the sensitivity of FujiLAM was 32·9% (95% CI 24·6-41·9; positive in 58 of 176 confirmed and unconfirmed samples) and the sensitivity of AlereLAM was 20·2% (12·3-29·4; 36 of 176).
33316214	17	107	theme	Research	2604:2611	arg1	Council					2613:2619	the UK Medical Research Council	2589:2619	the UK Medical Research Council	2589:2619	FUNDING German Federal Ministry of Education and Research, the Global Health Innovative Technology Fund, the UK Research and Innovation Global Challenges Research Fund, and the UK Medical Research Council.
33316214	17	107	theme	Research	2604:2611	arg1	Ministry					2439:2446	Ministry	2439:2446	Ministry	2439:2446	FUNDING German Federal Ministry of Education and Research, the Global Health Innovative Technology Fund, the UK Research and Innovation Global Challenges Research Fund, and the UK Medical Research Council.
33316214	12	108	theme	AlereLAM	1757:1764	arg1	%					1774:1774	87·8%	1770:1774	87·8%	1770:1774	The specificity of FujiLAM was 83·8% (95% CI 76·5-89·4; negative in 297 of 352 unconfirmed and unlikely samples) and the specificity of AlereLAM was 87·8% (79·0-93·7; 312 of 352).
33316214	12	108	theme	AlereLAM	1757:1764	arg1	specificity					1742:1752	the specificity	1738:1752	the specificity of AlereLAM	1738:1764	The specificity of FujiLAM was 83·8% (95% CI 76·5-89·4; negative in 297 of 352 unconfirmed and unlikely samples) and the specificity of AlereLAM was 87·8% (79·0-93·7; 312 of 352).
33316214	14	109	dep	negative	2117:2124	arg1	CI					2103:2104	95% CI 71·8-91·7	2099:2114	95% CI 71·8-91·7	2099:2114	The specificity of FujiLAM was 83·3% (95% CI 71·8-91·7; negative in 202 of 239 unlikely samples) and the specificity of AlereLAM was 90·0% (81·6-95·6; 216 of 239).
33316214	13	110	dep	12·3-29·4	2038:2046	arg1	176					2055:2057	176	2055:2057	176	2055:2057	Against the composite reference standard, both assays had decreased sensitivity; the sensitivity of FujiLAM was 32·9% (95% CI 24·6-41·9; positive in 58 of 176 confirmed and unconfirmed samples) and the sensitivity of AlereLAM was 20·2% (12·3-29·4; 36 of 176).
33316214	0	111	theme	lipoarabinomannan	22:38	arg1	tests					46:50	lipoarabinomannan urine tests	22:50	lipoarabinomannan urine tests	22:50	Comparing accuracy of lipoarabinomannan urine tests for diagnosis of pulmonary tuberculosis in children from four African countries: a cross-sectional study.
33316214	13	112	dep	%	2035:2035	arg1	12·3-29·4					2038:2046	12·3-29·4	2038:2046	12·3-29·4	2038:2046	Against the composite reference standard, both assays had decreased sensitivity; the sensitivity of FujiLAM was 32·9% (95% CI 24·6-41·9; positive in 58 of 176 confirmed and unconfirmed samples) and the sensitivity of AlereLAM was 20·2% (12·3-29·4; 36 of 176).
33316214	12	113	dep	negative	1677:1684	arg1	CI					1663:1664	95% CI 76·5-89·4	1659:1674	95% CI 76·5-89·4	1659:1674	The specificity of FujiLAM was 83·8% (95% CI 76·5-89·4; negative in 297 of 352 unconfirmed and unlikely samples) and the specificity of AlereLAM was 87·8% (79·0-93·7; 312 of 352).
33316214	2	114	theme	TB	432:433	arg1	Ag					439:440	TB LAM Ag	432:440	TB LAM Ag	432:440	We assessed side by side the diagnostic accuracy of the urine-based lipoarabinomannan assays Fujifilm SILVAMP TB LAM (FujiLAM) and Alere Determine TB LAM Ag (AlereLAM) for detection of childhood tuberculosis.
33316214	0	115	theme	tests	46:50	arg1	accuracy					10:17	accuracy	10:17	accuracy of lipoarabinomannan urine tests for diagnosis of pulmonary tuberculosis	10:90	Comparing accuracy of lipoarabinomannan urine tests for diagnosis of pulmonary tuberculosis in children from four African countries: a cross-sectional study.
33316214	17	116	theme	Global	2479:2484	arg1	Ministry					2439:2446	Ministry	2439:2446	Ministry	2439:2446	FUNDING German Federal Ministry of Education and Research, the Global Health Innovative Technology Fund, the UK Research and Innovation Global Challenges Research Fund, and the UK Medical Research Council.
33316214	17	116	theme	Global	2479:2484	arg1	Fund					2515:2518	the Global Health Innovative Technology Fund	2475:2518	the Global Health Innovative Technology Fund	2475:2518	FUNDING German Federal Ministry of Education and Research, the Global Health Innovative Technology Fund, the UK Research and Innovation Global Challenges Research Fund, and the UK Medical Research Council.
33316214	2	117	dep	Alere	416:420	arg1	Determine					422:430	Determine	422:430	Determine TB LAM Ag	422:440	We assessed side by side the diagnostic accuracy of the urine-based lipoarabinomannan assays Fujifilm SILVAMP TB LAM (FujiLAM) and Alere Determine TB LAM Ag (AlereLAM) for detection of childhood tuberculosis.
33316214	6	118	theme	diagnostic	869:878	arg1	performance					880:890	diagnostic performance	869:890	diagnostic performance against a microbiological reference standard (confirmed tuberculosis)	869:960	We measured diagnostic performance against a microbiological reference standard (confirmed tuberculosis) and a composite reference standard (confirmed and unconfirmed tuberculosis).
33316214	17	119	theme	Innovative	2493:2502	arg1	Ministry					2439:2446	Ministry	2439:2446	Ministry	2439:2446	FUNDING German Federal Ministry of Education and Research, the Global Health Innovative Technology Fund, the UK Research and Innovation Global Challenges Research Fund, and the UK Medical Research Council.
33316214	17	119	theme	Innovative	2493:2502	arg1	Fund					2515:2518	the Global Health Innovative Technology Fund	2475:2518	the Global Health Innovative Technology Fund	2475:2518	FUNDING German Federal Ministry of Education and Research, the Global Health Innovative Technology Fund, the UK Research and Innovation Global Challenges Research Fund, and the UK Medical Research Council.
33316214	2	120	theme	lipoarabinomannan	353:369	arg1	assays					371:376	the urine-based lipoarabinomannan assays Fujifilm SILVAMP TB LAM (FujiLAM)	337:410	the urine-based lipoarabinomannan assays Fujifilm SILVAMP TB LAM (FujiLAM)	337:410	We assessed side by side the diagnostic accuracy of the urine-based lipoarabinomannan assays Fujifilm SILVAMP TB LAM (FujiLAM) and Alere Determine TB LAM Ag (AlereLAM) for detection of childhood tuberculosis.
33316214	11	121	dep	%	1597:1597	arg1	8·6-61·6					1600:1607	8·6-61·6	1600:1607	8·6-61·6	1600:1607	Using the microbiological reference standard, the sensitivity of FujiLAM was 64·9% (95% CI 43·7-85·2; positive in 40 of 63 confirmed samples) and the sensitivity of AlereLAM was 30·7% (8·6-61·6; 19 of 63).
33316214	12	122	from	negative	1677:1684	arg1	samples					1725:1731	297 of 352 unconfirmed and unlikely samples	1689:1731	297 of 352 unconfirmed and unlikely samples	1689:1731	The specificity of FujiLAM was 83·8% (95% CI 76·5-89·4; negative in 297 of 352 unconfirmed and unlikely samples) and the specificity of AlereLAM was 87·8% (79·0-93·7; 312 of 352).
33316214	13	123	contain	had	1855:1857	arg1	assays					1848:1853	both assays	1843:1853	both assays	1843:1853	Against the composite reference standard, both assays had decreased sensitivity; the sensitivity of FujiLAM was 32·9% (95% CI 24·6-41·9; positive in 58 of 176 confirmed and unconfirmed samples) and the sensitivity of AlereLAM was 20·2% (12·3-29·4; 36 of 176).
33316214	13	123	contain	had	1855:1857	arg2	sensitivity					1869:1879	decreased sensitivity	1859:1879	decreased sensitivity	1859:1879	Against the composite reference standard, both assays had decreased sensitivity; the sensitivity of FujiLAM was 32·9% (95% CI 24·6-41·9; positive in 58 of 176 confirmed and unconfirmed samples) and the sensitivity of AlereLAM was 20·2% (12·3-29·4; 36 of 176).
33316214	11	124	dep	8·6-61·6	1600:1607	arg1	63					1616:1617	63	1616:1617	63	1616:1617	Using the microbiological reference standard, the sensitivity of FujiLAM was 64·9% (95% CI 43·7-85·2; positive in 40 of 63 confirmed samples) and the sensitivity of AlereLAM was 30·7% (8·6-61·6; 19 of 63).
33316214	0	125	from	countries	122:130	arg1	children					95:102	children	95:102	children from four African countries	95:130	Comparing accuracy of lipoarabinomannan urine tests for diagnosis of pulmonary tuberculosis in children from four African countries: a cross-sectional study.
33316214	2	126	theme	TB	395:396	arg1	FujiLAM					403:409	FujiLAM	403:409	FujiLAM	403:409	We assessed side by side the diagnostic accuracy of the urine-based lipoarabinomannan assays Fujifilm SILVAMP TB LAM (FujiLAM) and Alere Determine TB LAM Ag (AlereLAM) for detection of childhood tuberculosis.
33316214	2	126	theme	TB	395:396	arg1	LAM					398:400	Fujifilm SILVAMP TB LAM	378:400	the urine-based lipoarabinomannan assays Fujifilm SILVAMP TB LAM (FujiLAM)	337:410	We assessed side by side the diagnostic accuracy of the urine-based lipoarabinomannan assays Fujifilm SILVAMP TB LAM (FujiLAM) and Alere Determine TB LAM Ag (AlereLAM) for detection of childhood tuberculosis.
33316214	13	127	theme	unconfirmed	1974:1984	arg1	samples					1986:1992	58 of 176 confirmed and unconfirmed samples	1950:1992	58 of 176 confirmed and unconfirmed samples	1950:1992	Against the composite reference standard, both assays had decreased sensitivity; the sensitivity of FujiLAM was 32·9% (95% CI 24·6-41·9; positive in 58 of 176 confirmed and unconfirmed samples) and the sensitivity of AlereLAM was 20·2% (12·3-29·4; 36 of 176).
34364592	0	0	theme	stem	90:93	arg1	cells					95:99	stem cells	90:99	stem cells	90:99	Gradient chondroitin sulfate/poly (γ-glutamic acid) hydrogels inducing differentiation of stem cells for cartilage tissue engineering.
34364592	1	1	theme	γ-glutamic	333:342	arg1	acid					344:347	γ-glutamic acid	333:347	γ-glutamic acid	333:347	Based on the gradient distribution of structure and composition in biological cartilage tissue, we designed a gradient hydrogel scaffold by the moving photomask, using chondroitin sulfate and poly (γ-glutamic acid) as crude materials.
34364592	1	1	theme	γ-glutamic	333:342	arg1	poly					327:330	poly	327:330	poly (γ-glutamic acid)	327:348	Based on the gradient distribution of structure and composition in biological cartilage tissue, we designed a gradient hydrogel scaffold by the moving photomask, using chondroitin sulfate and poly (γ-glutamic acid) as crude materials.
34364592	1	2	theme	hydrogel	254:261	arg1	scaffold					263:270	a gradient hydrogel scaffold	243:270	a gradient hydrogel scaffold	243:270	Based on the gradient distribution of structure and composition in biological cartilage tissue, we designed a gradient hydrogel scaffold by the moving photomask, using chondroitin sulfate and poly (γ-glutamic acid) as crude materials.
34364592	5	3	theme	cartilage	1042:1050	arg1	function					1022:1029	the function	1018:1029	the function of natural cartilage than the homogeneous one	1018:1075	Remarkably, the results of in vitro stem cell differentiation experiments showed that the duration of light directly affected the differentiation extent of stem cells, demonstrating that the gradient hydrogel scaffold can better simulate the function of natural cartilage than the homogeneous one.
34364592	1	4	from	distribution	157:168	arg1	tissue					223:228	biological cartilage tissue	202:228	biological cartilage tissue	202:228	Based on the gradient distribution of structure and composition in biological cartilage tissue, we designed a gradient hydrogel scaffold by the moving photomask, using chondroitin sulfate and poly (γ-glutamic acid) as crude materials.
34364592	6	5	theme	cartilage	1171:1179	arg1	engineering					1188:1198	cartilage tissue engineering	1171:1198	cartilage tissue engineering	1171:1198	Due to these outstanding characteristics, this gradient hydrogel is a potential scaffold for cartilage tissue engineering.
34364592	5	6	dep	in	807:808	arg1	vitro					810:814	vitro	810:814	vitro	810:814	Remarkably, the results of in vitro stem cell differentiation experiments showed that the duration of light directly affected the differentiation extent of stem cells, demonstrating that the gradient hydrogel scaffold can better simulate the function of natural cartilage than the homogeneous one.
34364592	4	7	theme	live/dead	720:728	arg1	staining					730:737	live/dead staining	720:737	live/dead staining assays	720:744	Additionally, the gradient hydrogel's superior cell compatibility was proved through the MTT, live/dead staining assays, and 3D cell culture experiments.
34364592	5	8	theme	differentiation	910:924	arg1	extent					926:931	the differentiation extent	906:931	the differentiation extent of stem cells	906:945	Remarkably, the results of in vitro stem cell differentiation experiments showed that the duration of light directly affected the differentiation extent of stem cells, demonstrating that the gradient hydrogel scaffold can better simulate the function of natural cartilage than the homogeneous one.
34364592	0	9	theme	cells	95:99	arg1	differentiation					71:85	differentiation	71:85	differentiation of stem cells for cartilage tissue engineering	71:132	Gradient chondroitin sulfate/poly (γ-glutamic acid) hydrogels inducing differentiation of stem cells for cartilage tissue engineering.
34364592	5	10	theme	in	807:808	arg1	experiments					842:852	in vitro stem cell differentiation experiments	807:852	in vitro stem cell differentiation experiments	807:852	Remarkably, the results of in vitro stem cell differentiation experiments showed that the duration of light directly affected the differentiation extent of stem cells, demonstrating that the gradient hydrogel scaffold can better simulate the function of natural cartilage than the homogeneous one.
34364592	0	11	theme	tissue	115:120	arg1	engineering					122:132	cartilage tissue engineering	105:132	cartilage tissue engineering	105:132	Gradient chondroitin sulfate/poly (γ-glutamic acid) hydrogels inducing differentiation of stem cells for cartilage tissue engineering.
34364592	1	12	theme	crude	353:357	arg1	sulfate					315:321	chondroitin sulfate	303:321	chondroitin sulfate	303:321	Based on the gradient distribution of structure and composition in biological cartilage tissue, we designed a gradient hydrogel scaffold by the moving photomask, using chondroitin sulfate and poly (γ-glutamic acid) as crude materials.
34364592	1	12	theme	crude	353:357	arg1	materials					359:367	crude materials	353:367	crude materials	353:367	Based on the gradient distribution of structure and composition in biological cartilage tissue, we designed a gradient hydrogel scaffold by the moving photomask, using chondroitin sulfate and poly (γ-glutamic acid) as crude materials.
34364592	1	12	theme	crude	353:357	arg1	poly					327:330	poly	327:330	poly (γ-glutamic acid)	327:348	Based on the gradient distribution of structure and composition in biological cartilage tissue, we designed a gradient hydrogel scaffold by the moving photomask, using chondroitin sulfate and poly (γ-glutamic acid) as crude materials.
34364592	4	13	theme	assays	739:744	arg1	experiments					767:777	the MTT, live/dead staining assays, and 3D cell culture experiments	711:777	the MTT, live/dead staining assays, and 3D cell culture experiments	711:777	Additionally, the gradient hydrogel's superior cell compatibility was proved through the MTT, live/dead staining assays, and 3D cell culture experiments.
34364592	2	14	theme	hydrogel	374:381	arg1	scaffold					383:390	The hydrogel scaffold	370:390	The hydrogel scaffold	370:390	The hydrogel scaffold had a gradient distribution of cross-linking density, which can be verified from the results of SEM and swelling behavior.
34364592	1	15	theme	structure	173:181	arg1	distribution					157:168	the gradient distribution	144:168	the gradient distribution of structure and composition in biological cartilage tissue	144:228	Based on the gradient distribution of structure and composition in biological cartilage tissue, we designed a gradient hydrogel scaffold by the moving photomask, using chondroitin sulfate and poly (γ-glutamic acid) as crude materials.
34364592	0	16	theme	cartilage	105:113	arg1	engineering					122:132	cartilage tissue engineering	105:132	cartilage tissue engineering	105:132	Gradient chondroitin sulfate/poly (γ-glutamic acid) hydrogels inducing differentiation of stem cells for cartilage tissue engineering.
34364592	4	17	theme	staining	730:737	arg1	assays					739:744	live/dead staining assays	720:744	live/dead staining assays	720:744	Additionally, the gradient hydrogel's superior cell compatibility was proved through the MTT, live/dead staining assays, and 3D cell culture experiments.
34364592	2	18	theme	swelling	496:503	arg1	behavior					505:512	swelling behavior	496:512	swelling behavior	496:512	The hydrogel scaffold had a gradient distribution of cross-linking density, which can be verified from the results of SEM and swelling behavior.
34364592	0	19	theme	chondroitin	9:19	arg1	sulfate/poly					21:32	Gradient chondroitin sulfate/poly	0:32	Gradient chondroitin sulfate/poly (γ-glutamic acid)	0:50	Gradient chondroitin sulfate/poly (γ-glutamic acid) hydrogels inducing differentiation of stem cells for cartilage tissue engineering.
34364592	0	19	theme	chondroitin	9:19	arg1	acid					46:49	γ-glutamic acid	35:49	γ-glutamic acid	35:49	Gradient chondroitin sulfate/poly (γ-glutamic acid) hydrogels inducing differentiation of stem cells for cartilage tissue engineering.
34364592	6	20	theme	gradient	1125:1132	arg1	scaffold					1158:1165	a potential scaffold	1146:1165	a potential scaffold for cartilage tissue engineering	1146:1198	Due to these outstanding characteristics, this gradient hydrogel is a potential scaffold for cartilage tissue engineering.
34364592	6	20	theme	gradient	1125:1132	arg1	hydrogel					1134:1141	this gradient hydrogel	1120:1141	this gradient hydrogel	1120:1141	Due to these outstanding characteristics, this gradient hydrogel is a potential scaffold for cartilage tissue engineering.
34364592	3	21	theme	viscoelastic	553:564	arg1	toughness					567:575	toughness	567:575	toughness (70% strain)	567:588	Besides, the hydrogel exhibited great viscoelastic, toughness (70% strain), and strength properties (600 kPa).
34364592	3	21	theme	viscoelastic	553:564	arg1	strain					582:587	70% strain	578:587	70% strain	578:587	Besides, the hydrogel exhibited great viscoelastic, toughness (70% strain), and strength properties (600 kPa).
34364592	5	22	theme	homogeneous	1061:1071	arg1	one					1073:1075	the homogeneous one	1057:1075	the homogeneous one	1057:1075	Remarkably, the results of in vitro stem cell differentiation experiments showed that the duration of light directly affected the differentiation extent of stem cells, demonstrating that the gradient hydrogel scaffold can better simulate the function of natural cartilage than the homogeneous one.
34364592	1	23	theme	composition	187:197	arg1	distribution					157:168	the gradient distribution	144:168	the gradient distribution of structure and composition in biological cartilage tissue	144:228	Based on the gradient distribution of structure and composition in biological cartilage tissue, we designed a gradient hydrogel scaffold by the moving photomask, using chondroitin sulfate and poly (γ-glutamic acid) as crude materials.
34364592	0	24	theme	Gradient	0:7	arg1	sulfate/poly					21:32	Gradient chondroitin sulfate/poly	0:32	Gradient chondroitin sulfate/poly (γ-glutamic acid)	0:50	Gradient chondroitin sulfate/poly (γ-glutamic acid) hydrogels inducing differentiation of stem cells for cartilage tissue engineering.
34364592	0	24	theme	Gradient	0:7	arg1	acid					46:49	γ-glutamic acid	35:49	γ-glutamic acid	35:49	Gradient chondroitin sulfate/poly (γ-glutamic acid) hydrogels inducing differentiation of stem cells for cartilage tissue engineering.
34364592	1	25	theme	moving	279:284	arg1	photomask					286:294	the moving photomask	275:294	the moving photomask	275:294	Based on the gradient distribution of structure and composition in biological cartilage tissue, we designed a gradient hydrogel scaffold by the moving photomask, using chondroitin sulfate and poly (γ-glutamic acid) as crude materials.
34364592	5	26	theme	cell	821:824	arg1	experiments					842:852	in vitro stem cell differentiation experiments	807:852	in vitro stem cell differentiation experiments	807:852	Remarkably, the results of in vitro stem cell differentiation experiments showed that the duration of light directly affected the differentiation extent of stem cells, demonstrating that the gradient hydrogel scaffold can better simulate the function of natural cartilage than the homogeneous one.
34364592	2	27	theme	density	437:443	arg1	distribution					407:418	a gradient distribution	396:418	a gradient distribution	396:418	The hydrogel scaffold had a gradient distribution of cross-linking density, which can be verified from the results of SEM and swelling behavior.
34364592	4	28	theme	MTT	715:717	arg1	experiments					767:777	the MTT, live/dead staining assays, and 3D cell culture experiments	711:777	the MTT, live/dead staining assays, and 3D cell culture experiments	711:777	Additionally, the gradient hydrogel's superior cell compatibility was proved through the MTT, live/dead staining assays, and 3D cell culture experiments.
34364592	5	29	theme	gradient	971:978	arg1	scaffold					989:996	the gradient hydrogel scaffold	967:996	the gradient hydrogel scaffold	967:996	Remarkably, the results of in vitro stem cell differentiation experiments showed that the duration of light directly affected the differentiation extent of stem cells, demonstrating that the gradient hydrogel scaffold can better simulate the function of natural cartilage than the homogeneous one.
34364592	1	30	theme	biological	202:211	arg1	tissue					223:228	biological cartilage tissue	202:228	biological cartilage tissue	202:228	Based on the gradient distribution of structure and composition in biological cartilage tissue, we designed a gradient hydrogel scaffold by the moving photomask, using chondroitin sulfate and poly (γ-glutamic acid) as crude materials.
34364592	2	31	theme	cross-linking	423:435	arg1	density					437:443	cross-linking density	423:443	cross-linking density	423:443	The hydrogel scaffold had a gradient distribution of cross-linking density, which can be verified from the results of SEM and swelling behavior.
34364592	5	32	theme	stem	936:939	arg1	cells					941:945	stem cells	936:945	stem cells	936:945	Remarkably, the results of in vitro stem cell differentiation experiments showed that the duration of light directly affected the differentiation extent of stem cells, demonstrating that the gradient hydrogel scaffold can better simulate the function of natural cartilage than the homogeneous one.
34364592	3	33	theme	great	547:551	arg1	toughness					567:575	toughness	567:575	toughness (70% strain)	567:588	Besides, the hydrogel exhibited great viscoelastic, toughness (70% strain), and strength properties (600 kPa).
34364592	3	33	theme	great	547:551	arg1	strain					582:587	70% strain	578:587	70% strain	578:587	Besides, the hydrogel exhibited great viscoelastic, toughness (70% strain), and strength properties (600 kPa).
34364592	1	34	theme	cartilage	213:221	arg1	tissue					223:228	biological cartilage tissue	202:228	biological cartilage tissue	202:228	Based on the gradient distribution of structure and composition in biological cartilage tissue, we designed a gradient hydrogel scaffold by the moving photomask, using chondroitin sulfate and poly (γ-glutamic acid) as crude materials.
34364592	5	35	theme	natural	1034:1040	arg1	cartilage					1042:1050	natural cartilage	1034:1050	natural cartilage	1034:1050	Remarkably, the results of in vitro stem cell differentiation experiments showed that the duration of light directly affected the differentiation extent of stem cells, demonstrating that the gradient hydrogel scaffold can better simulate the function of natural cartilage than the homogeneous one.
34364592	3	36	theme	70	578:579	arg1	%					580:580	%	580:580	%	580:580	Besides, the hydrogel exhibited great viscoelastic, toughness (70% strain), and strength properties (600 kPa).
34364592	2	37	theme	gradient	398:405	arg1	distribution					407:418	a gradient distribution	396:418	a gradient distribution	396:418	The hydrogel scaffold had a gradient distribution of cross-linking density, which can be verified from the results of SEM and swelling behavior.
34364592	0	38	theme	γ-glutamic	35:44	arg1	sulfate/poly					21:32	Gradient chondroitin sulfate/poly	0:32	Gradient chondroitin sulfate/poly (γ-glutamic acid)	0:50	Gradient chondroitin sulfate/poly (γ-glutamic acid) hydrogels inducing differentiation of stem cells for cartilage tissue engineering.
34364592	0	38	theme	γ-glutamic	35:44	arg1	acid					46:49	γ-glutamic acid	35:49	γ-glutamic acid	35:49	Gradient chondroitin sulfate/poly (γ-glutamic acid) hydrogels inducing differentiation of stem cells for cartilage tissue engineering.
34364592	1	39	theme	chondroitin	303:313	arg1	sulfate					315:321	chondroitin sulfate	303:321	chondroitin sulfate	303:321	Based on the gradient distribution of structure and composition in biological cartilage tissue, we designed a gradient hydrogel scaffold by the moving photomask, using chondroitin sulfate and poly (γ-glutamic acid) as crude materials.
34364592	1	39	theme	chondroitin	303:313	arg1	materials					359:367	crude materials	353:367	crude materials	353:367	Based on the gradient distribution of structure and composition in biological cartilage tissue, we designed a gradient hydrogel scaffold by the moving photomask, using chondroitin sulfate and poly (γ-glutamic acid) as crude materials.
34364592	1	39	theme	chondroitin	303:313	arg1	poly					327:330	poly	327:330	poly (γ-glutamic acid)	327:348	Based on the gradient distribution of structure and composition in biological cartilage tissue, we designed a gradient hydrogel scaffold by the moving photomask, using chondroitin sulfate and poly (γ-glutamic acid) as crude materials.
34364592	5	40	theme	stem	816:819	arg1	experiments					842:852	in vitro stem cell differentiation experiments	807:852	in vitro stem cell differentiation experiments	807:852	Remarkably, the results of in vitro stem cell differentiation experiments showed that the duration of light directly affected the differentiation extent of stem cells, demonstrating that the gradient hydrogel scaffold can better simulate the function of natural cartilage than the homogeneous one.
34364592	3	41	theme	strength	595:602	arg1	600 kPa					616:622	600 kPa	616:622	600 kPa	616:622	Besides, the hydrogel exhibited great viscoelastic, toughness (70% strain), and strength properties (600 kPa).
34364592	3	41	theme	strength	595:602	arg1	properties					604:613	strength properties	595:613	strength properties (600 kPa)	595:623	Besides, the hydrogel exhibited great viscoelastic, toughness (70% strain), and strength properties (600 kPa).
34364592	3	42	theme	%	580:580	arg1	toughness					567:575	toughness	567:575	toughness (70% strain)	567:588	Besides, the hydrogel exhibited great viscoelastic, toughness (70% strain), and strength properties (600 kPa).
34364592	3	42	theme	%	580:580	arg1	strain					582:587	70% strain	578:587	70% strain	578:587	Besides, the hydrogel exhibited great viscoelastic, toughness (70% strain), and strength properties (600 kPa).
34364592	5	43	theme	differentiation	826:840	arg1	experiments					842:852	in vitro stem cell differentiation experiments	807:852	in vitro stem cell differentiation experiments	807:852	Remarkably, the results of in vitro stem cell differentiation experiments showed that the duration of light directly affected the differentiation extent of stem cells, demonstrating that the gradient hydrogel scaffold can better simulate the function of natural cartilage than the homogeneous one.
34364592	2	44	contain	had	392:394	arg1	scaffold					383:390	The hydrogel scaffold	370:390	The hydrogel scaffold	370:390	The hydrogel scaffold had a gradient distribution of cross-linking density, which can be verified from the results of SEM and swelling behavior.
34364592	2	44	contain	had	392:394	arg2	distribution					407:418	a gradient distribution	396:418	a gradient distribution	396:418	The hydrogel scaffold had a gradient distribution of cross-linking density, which can be verified from the results of SEM and swelling behavior.
34364592	2	45	theme	behavior	505:512	arg1	results					477:483	the results	473:483	the results of SEM and swelling behavior	473:512	The hydrogel scaffold had a gradient distribution of cross-linking density, which can be verified from the results of SEM and swelling behavior.
34364592	4	46	theme	cell	673:676	arg1	compatibility					678:690	the gradient hydrogel's superior cell compatibility	640:690	the gradient hydrogel's superior cell compatibility	640:690	Additionally, the gradient hydrogel's superior cell compatibility was proved through the MTT, live/dead staining assays, and 3D cell culture experiments.
34364592	4	47	theme	superior	664:671	arg1	compatibility					678:690	the gradient hydrogel's superior cell compatibility	640:690	the gradient hydrogel's superior cell compatibility	640:690	Additionally, the gradient hydrogel's superior cell compatibility was proved through the MTT, live/dead staining assays, and 3D cell culture experiments.
34364592	5	48	theme	hydrogel	980:987	arg1	scaffold					989:996	the gradient hydrogel scaffold	967:996	the gradient hydrogel scaffold	967:996	Remarkably, the results of in vitro stem cell differentiation experiments showed that the duration of light directly affected the differentiation extent of stem cells, demonstrating that the gradient hydrogel scaffold can better simulate the function of natural cartilage than the homogeneous one.
34364592	6	49	theme	tissue	1181:1186	arg1	engineering					1188:1198	cartilage tissue engineering	1171:1198	cartilage tissue engineering	1171:1198	Due to these outstanding characteristics, this gradient hydrogel is a potential scaffold for cartilage tissue engineering.
34364592	4	50	theme	culture	759:765	arg1	experiments					767:777	the MTT, live/dead staining assays, and 3D cell culture experiments	711:777	the MTT, live/dead staining assays, and 3D cell culture experiments	711:777	Additionally, the gradient hydrogel's superior cell compatibility was proved through the MTT, live/dead staining assays, and 3D cell culture experiments.
34364592	5	51	theme	cells	941:945	arg1	extent					926:931	the differentiation extent	906:931	the differentiation extent of stem cells	906:945	Remarkably, the results of in vitro stem cell differentiation experiments showed that the duration of light directly affected the differentiation extent of stem cells, demonstrating that the gradient hydrogel scaffold can better simulate the function of natural cartilage than the homogeneous one.
34364592	6	52	theme	potential	1148:1156	arg1	scaffold					1158:1165	a potential scaffold	1146:1165	a potential scaffold for cartilage tissue engineering	1146:1198	Due to these outstanding characteristics, this gradient hydrogel is a potential scaffold for cartilage tissue engineering.
34364592	6	52	theme	potential	1148:1156	arg1	hydrogel					1134:1141	this gradient hydrogel	1120:1141	this gradient hydrogel	1120:1141	Due to these outstanding characteristics, this gradient hydrogel is a potential scaffold for cartilage tissue engineering.
34364592	5	53	theme	light	882:886	arg1	duration					870:877	the duration	866:877	the duration of light	866:886	Remarkably, the results of in vitro stem cell differentiation experiments showed that the duration of light directly affected the differentiation extent of stem cells, demonstrating that the gradient hydrogel scaffold can better simulate the function of natural cartilage than the homogeneous one.
34364592	6	54	theme	outstanding	1091:1101	arg1	characteristics					1103:1117	these outstanding characteristics	1085:1117	these outstanding characteristics	1085:1117	Due to these outstanding characteristics, this gradient hydrogel is a potential scaffold for cartilage tissue engineering.
34364592	2	55	theme	SEM	488:490	arg1	results					477:483	the results	473:483	the results of SEM and swelling behavior	473:512	The hydrogel scaffold had a gradient distribution of cross-linking density, which can be verified from the results of SEM and swelling behavior.
34364592	5	56	theme	experiments	842:852	arg1	results					796:802	the results	792:802	the results of in vitro stem cell differentiation experiments	792:852	Remarkably, the results of in vitro stem cell differentiation experiments showed that the duration of light directly affected the differentiation extent of stem cells, demonstrating that the gradient hydrogel scaffold can better simulate the function of natural cartilage than the homogeneous one.
34364592	4	57	theme	cell	754:757	arg1	experiments					767:777	the MTT, live/dead staining assays, and 3D cell culture experiments	711:777	the MTT, live/dead staining assays, and 3D cell culture experiments	711:777	Additionally, the gradient hydrogel's superior cell compatibility was proved through the MTT, live/dead staining assays, and 3D cell culture experiments.
34364592	4	58	theme	gradient	644:651	arg1	compatibility					678:690	the gradient hydrogel's superior cell compatibility	640:690	the gradient hydrogel's superior cell compatibility	640:690	Additionally, the gradient hydrogel's superior cell compatibility was proved through the MTT, live/dead staining assays, and 3D cell culture experiments.
34364592	4	59	theme	3D	751:752	arg1	experiments					767:777	the MTT, live/dead staining assays, and 3D cell culture experiments	711:777	the MTT, live/dead staining assays, and 3D cell culture experiments	711:777	Additionally, the gradient hydrogel's superior cell compatibility was proved through the MTT, live/dead staining assays, and 3D cell culture experiments.
34364592	1	60	theme	gradient	148:155	arg1	distribution					157:168	the gradient distribution	144:168	the gradient distribution of structure and composition in biological cartilage tissue	144:228	Based on the gradient distribution of structure and composition in biological cartilage tissue, we designed a gradient hydrogel scaffold by the moving photomask, using chondroitin sulfate and poly (γ-glutamic acid) as crude materials.
34364592	1	61	theme	gradient	245:252	arg1	scaffold					263:270	a gradient hydrogel scaffold	243:270	a gradient hydrogel scaffold	243:270	Based on the gradient distribution of structure and composition in biological cartilage tissue, we designed a gradient hydrogel scaffold by the moving photomask, using chondroitin sulfate and poly (γ-glutamic acid) as crude materials.
33803094	4	0	from	vancomycin	703:712	arg1	not					739:741	not	739:741	not	739:741	A 2 × 2 factorial design was used, in which C57Bl/6J mice were fed either the standard AIN93G diet or TWD and with vancomycin in the drinking water or not.
33803094	4	0	from	vancomycin	703:712	arg1	water					730:734	the drinking water	717:734	the drinking water	717:734	A 2 × 2 factorial design was used, in which C57Bl/6J mice were fed either the standard AIN93G diet or TWD and with vancomycin in the drinking water or not.
33803094	4	1	theme	C57Bl/6J	632:639	arg1	mice					641:644	C57Bl/6J mice	632:644	C57Bl/6J mice	632:644	A 2 × 2 factorial design was used, in which C57Bl/6J mice were fed either the standard AIN93G diet or TWD and with vancomycin in the drinking water or not.
33803094	0	2	theme	Gut	77:79	arg1	Microbiome					81:90	the Gut Microbiome	73:90	the Gut Microbiome	73:90	The Western Dietary Pattern Combined with Vancomycin-Mediated Changes to the Gut Microbiome Exacerbates Colitis Severity and Colon Tumorigenesis.
33803094	8	3	theme	tumor	1467:1471	arg1	rates					1473:1477	higher tumor rates	1460:1477	higher tumor rates	1460:1477	These results support our previous research that the TWD promotes colon tumorigenesis and suggest that vancomycin-induced changes to the gut microbiome are associated with higher tumor rates.
33803094	8	4	theme	higher	1460:1465	arg1	rates					1473:1477	higher tumor rates	1460:1477	higher tumor rates	1460:1477	These results support our previous research that the TWD promotes colon tumorigenesis and suggest that vancomycin-induced changes to the gut microbiome are associated with higher tumor rates.
33803094	0	5	theme	Colitis	104:110	arg1	Severity					112:119	Colitis Severity	104:119	Colitis Severity	104:119	The Western Dietary Pattern Combined with Vancomycin-Mediated Changes to the Gut Microbiome Exacerbates Colitis Severity and Colon Tumorigenesis.
33803094	8	6	theme	colon	1354:1358	arg1	tumorigenesis					1360:1372	colon tumorigenesis	1354:1372	colon tumorigenesis	1354:1372	These results support our previous research that the TWD promotes colon tumorigenesis and suggest that vancomycin-induced changes to the gut microbiome are associated with higher tumor rates.
33803094	7	7	theme	microbiome	1264:1273	arg1	composition					1275:1285	the gut microbiome composition	1256:1285	the gut microbiome composition	1256:1285	Conversely, basal diet had relatively minor effects on the gut microbiome composition.
33803094	3	8	theme	colon	516:520	arg1	tumorigenesis					522:534	colon tumorigenesis	516:534	colon tumorigenesis in the context of a standard mouse diet or the TWD	516:585	Therefore, the objective of this study was to determine the impact of vancomycin on colon tumorigenesis in the context of a standard mouse diet or the TWD.
33803094	3	9	from	tumorigenesis	522:534	arg1	context					543:549	the context	539:549	the context of a standard mouse diet or the TWD	539:585	Therefore, the objective of this study was to determine the impact of vancomycin on colon tumorigenesis in the context of a standard mouse diet or the TWD.
33803094	1	10	theme	total	260:264	arg1	TWD					280:282	TWD	280:282	TWD	280:282	Previous work by our group using a mouse model of inflammation-associated colorectal cancer (CAC) showed that the total Western diet (TWD) promoted colon tumor development.
33803094	1	10	theme	total	260:264	arg1	diet					274:277	the total Western diet	256:277	the total Western diet (TWD)	256:283	Previous work by our group using a mouse model of inflammation-associated colorectal cancer (CAC) showed that the total Western diet (TWD) promoted colon tumor development.
33803094	3	11	from	impact	492:497	arg1	tumorigenesis					522:534	colon tumorigenesis	516:534	colon tumorigenesis in the context of a standard mouse diet or the TWD	516:585	Therefore, the objective of this study was to determine the impact of vancomycin on colon tumorigenesis in the context of a standard mouse diet or the TWD.
33803094	5	12	theme	water	904:908	arg1	controls					910:917	plain water controls	898:917	plain water controls	898:917	While both the TWD and vancomycin treatments independently increased parameters associated with gut inflammation and tumorigenesis compared to AIN93G and plain water controls, mice fed the TWD and treated with vancomycin had significantly increased tumor multiplicity and burden relative to all other treatments.
33803094	1	13	theme	Western	266:272	arg1	TWD					280:282	TWD	280:282	TWD	280:282	Previous work by our group using a mouse model of inflammation-associated colorectal cancer (CAC) showed that the total Western diet (TWD) promoted colon tumor development.
33803094	1	13	theme	Western	266:272	arg1	diet					274:277	the total Western diet	256:277	the total Western diet (TWD)	256:283	Previous work by our group using a mouse model of inflammation-associated colorectal cancer (CAC) showed that the total Western diet (TWD) promoted colon tumor development.
33803094	7	14	theme	basal	1213:1217	arg1	diet					1219:1222	basal diet	1213:1222	basal diet	1213:1222	Conversely, basal diet had relatively minor effects on the gut microbiome composition.
33803094	3	15	theme	vancomycin	502:511	arg1	impact					492:497	the impact	488:497	the impact of vancomycin on colon tumorigenesis in the context of a standard mouse diet or the TWD	488:585	Therefore, the objective of this study was to determine the impact of vancomycin on colon tumorigenesis in the context of a standard mouse diet or the TWD.
33803094	7	16	theme	gut	1260:1262	arg1	composition					1275:1285	the gut microbiome composition	1256:1285	the gut microbiome composition	1256:1285	Conversely, basal diet had relatively minor effects on the gut microbiome composition.
33803094	5	17	contain	had	965:967	arg1	mice					920:923	mice	920:923	mice fed the TWD and treated with vancomycin	920:963	While both the TWD and vancomycin treatments independently increased parameters associated with gut inflammation and tumorigenesis compared to AIN93G and plain water controls, mice fed the TWD and treated with vancomycin had significantly increased tumor multiplicity and burden relative to all other treatments.
33803094	5	17	contain	had	965:967	arg2	burden					1016:1021	burden	1016:1021	burden	1016:1021	While both the TWD and vancomycin treatments independently increased parameters associated with gut inflammation and tumorigenesis compared to AIN93G and plain water controls, mice fed the TWD and treated with vancomycin had significantly increased tumor multiplicity and burden relative to all other treatments.
33803094	5	17	contain	had	965:967	arg2	multiplicity					999:1010	significantly increased tumor multiplicity	969:1010	significantly increased tumor multiplicity	969:1010	While both the TWD and vancomycin treatments independently increased parameters associated with gut inflammation and tumorigenesis compared to AIN93G and plain water controls, mice fed the TWD and treated with vancomycin had significantly increased tumor multiplicity and burden relative to all other treatments.
33803094	5	18	theme	other	1039:1043	arg1	treatments					1045:1054	all other treatments	1035:1054	all other treatments	1035:1054	While both the TWD and vancomycin treatments independently increased parameters associated with gut inflammation and tumorigenesis compared to AIN93G and plain water controls, mice fed the TWD and treated with vancomycin had significantly increased tumor multiplicity and burden relative to all other treatments.
33803094	4	19	theme	factorial	596:604	arg1	design					606:611	A 2 × 2 factorial design	588:611	A 2 × 2 factorial design	588:611	A 2 × 2 factorial design was used, in which C57Bl/6J mice were fed either the standard AIN93G diet or TWD and with vancomycin in the drinking water or not.
33803094	0	20	theme	Western	4:10	arg1	Pattern					20:26	The Western Dietary Pattern	0:26	The Western Dietary Pattern Combined with Vancomycin-Mediated Changes to the Gut Microbiome	0:90	The Western Dietary Pattern Combined with Vancomycin-Mediated Changes to the Gut Microbiome Exacerbates Colitis Severity and Colon Tumorigenesis.
33803094	6	21	theme	genus	1187:1191	arg1	levels					1193:1198	the phylum, family, and genus levels	1163:1198	levels	1193:1198	Vancomycin treatment significantly decreased alpha diversity and changed the abundance of several taxa at the phylum, family, and genus levels.
33803094	4	22	theme	standard	666:673	arg1	diet					682:685	the standard AIN93G diet	662:685	the standard AIN93G diet	662:685	A 2 × 2 factorial design was used, in which C57Bl/6J mice were fed either the standard AIN93G diet or TWD and with vancomycin in the drinking water or not.
33803094	1	23	theme	mouse	181:185	arg1	model					187:191	a mouse model	179:191	a mouse model of inflammation-associated colorectal cancer (CAC)	179:242	Previous work by our group using a mouse model of inflammation-associated colorectal cancer (CAC) showed that the total Western diet (TWD) promoted colon tumor development.
33803094	5	24	theme	tumor	993:997	arg1	multiplicity					999:1010	significantly increased tumor multiplicity	969:1010	significantly increased tumor multiplicity	969:1010	While both the TWD and vancomycin treatments independently increased parameters associated with gut inflammation and tumorigenesis compared to AIN93G and plain water controls, mice fed the TWD and treated with vancomycin had significantly increased tumor multiplicity and burden relative to all other treatments.
33803094	5	25	theme	vancomycin	767:776	arg1	treatments					778:787	both the TWD and vancomycin treatments	750:787	treatments	778:787	While both the TWD and vancomycin treatments independently increased parameters associated with gut inflammation and tumorigenesis compared to AIN93G and plain water controls, mice fed the TWD and treated with vancomycin had significantly increased tumor multiplicity and burden relative to all other treatments.
33803094	7	26	contain	had	1224:1226	arg2	effects					1245:1251	relatively minor effects	1228:1251	relatively minor effects	1228:1251	Conversely, basal diet had relatively minor effects on the gut microbiome composition.
33803094	7	26	contain	had	1224:1226	arg1	diet					1219:1222	basal diet	1213:1222	basal diet	1213:1222	Conversely, basal diet had relatively minor effects on the gut microbiome composition.
33803094	6	27	theme	taxa	1155:1158	arg1	abundance					1134:1142	the abundance	1130:1142	the abundance of several taxa at the phylum, family, and genus levels	1130:1198	Vancomycin treatment significantly decreased alpha diversity and changed the abundance of several taxa at the phylum, family, and genus levels.
33803094	6	28	theme	phylum	1167:1172	arg1	family					1175:1180	the phylum, family, and genus levels	1163:1198	family	1175:1180	Vancomycin treatment significantly decreased alpha diversity and changed the abundance of several taxa at the phylum, family, and genus levels.
33803094	6	29	theme	alpha	1102:1106	arg1	diversity					1108:1116	alpha diversity	1102:1116	alpha diversity	1102:1116	Vancomycin treatment significantly decreased alpha diversity and changed the abundance of several taxa at the phylum, family, and genus levels.
33803094	7	30	theme	minor	1239:1243	arg1	effects					1245:1251	relatively minor effects	1228:1251	relatively minor effects	1228:1251	Conversely, basal diet had relatively minor effects on the gut microbiome composition.
33803094	0	31	theme	Dietary	12:18	arg1	Pattern					20:26	The Western Dietary Pattern	0:26	The Western Dietary Pattern Combined with Vancomycin-Mediated Changes to the Gut Microbiome	0:90	The Western Dietary Pattern Combined with Vancomycin-Mediated Changes to the Gut Microbiome Exacerbates Colitis Severity and Colon Tumorigenesis.
33803094	0	32	theme	Colon	125:129	arg1	Tumorigenesis					131:143	Colon Tumorigenesis	125:143	Colon Tumorigenesis	125:143	The Western Dietary Pattern Combined with Vancomycin-Mediated Changes to the Gut Microbiome Exacerbates Colitis Severity and Colon Tumorigenesis.
33803094	8	33	theme	vancomycin-induced	1391:1408	arg1	changes					1410:1416	vancomycin-induced changes	1391:1416	vancomycin-induced changes to the gut microbiome	1391:1438	These results support our previous research that the TWD promotes colon tumorigenesis and suggest that vancomycin-induced changes to the gut microbiome are associated with higher tumor rates.
33803094	4	34	theme	AIN93G	675:680	arg1	diet					682:685	the standard AIN93G diet	662:685	the standard AIN93G diet	662:685	A 2 × 2 factorial design was used, in which C57Bl/6J mice were fed either the standard AIN93G diet or TWD and with vancomycin in the drinking water or not.
33803094	1	35	theme	inflammation-associated	196:218	arg1	CAC					239:241	CAC	239:241	CAC	239:241	Previous work by our group using a mouse model of inflammation-associated colorectal cancer (CAC) showed that the total Western diet (TWD) promoted colon tumor development.
33803094	1	35	theme	inflammation-associated	196:218	arg1	cancer					231:236	inflammation-associated colorectal cancer	196:236	inflammation-associated colorectal cancer (CAC)	196:242	Previous work by our group using a mouse model of inflammation-associated colorectal cancer (CAC) showed that the total Western diet (TWD) promoted colon tumor development.
33803094	1	36	theme	colorectal	220:229	arg1	CAC					239:241	CAC	239:241	CAC	239:241	Previous work by our group using a mouse model of inflammation-associated colorectal cancer (CAC) showed that the total Western diet (TWD) promoted colon tumor development.
33803094	1	36	theme	colorectal	220:229	arg1	cancer					231:236	inflammation-associated colorectal cancer	196:236	inflammation-associated colorectal cancer (CAC)	196:242	Previous work by our group using a mouse model of inflammation-associated colorectal cancer (CAC) showed that the total Western diet (TWD) promoted colon tumor development.
33803094	3	37	theme	mouse	565:569	arg1	diet					571:574	a standard mouse diet	554:574	a standard mouse diet	554:574	Therefore, the objective of this study was to determine the impact of vancomycin on colon tumorigenesis in the context of a standard mouse diet or the TWD.
33803094	6	38	from	family	1175:1180	arg1	abundance					1134:1142	the abundance	1130:1142	the abundance of several taxa at the phylum, family, and genus levels	1130:1198	Vancomycin treatment significantly decreased alpha diversity and changed the abundance of several taxa at the phylum, family, and genus levels.
33803094	2	39	theme	colorectal	407:416	arg1	CRC					426:428	CRC	426:428	CRC	426:428	Others have also shown that vancomycin-mediated changes to the gut microbiome increased colorectal cancer (CRC).
33803094	2	39	theme	colorectal	407:416	arg1	cancer					418:423	colorectal cancer	407:423	colorectal cancer (CRC)	407:429	Others have also shown that vancomycin-mediated changes to the gut microbiome increased colorectal cancer (CRC).
33803094	5	40	dep	mice	920:923	arg1	treated					941:947	treated	941:947	treated with vancomycin	941:963	While both the TWD and vancomycin treatments independently increased parameters associated with gut inflammation and tumorigenesis compared to AIN93G and plain water controls, mice fed the TWD and treated with vancomycin had significantly increased tumor multiplicity and burden relative to all other treatments.
33803094	5	40	dep	mice	920:923	arg1	fed					925:927	fed	925:927	fed the TWD	925:935	While both the TWD and vancomycin treatments independently increased parameters associated with gut inflammation and tumorigenesis compared to AIN93G and plain water controls, mice fed the TWD and treated with vancomycin had significantly increased tumor multiplicity and burden relative to all other treatments.
33803094	3	41	theme	diet	571:574	arg1	context					543:549	the context	539:549	the context of a standard mouse diet or the TWD	539:585	Therefore, the objective of this study was to determine the impact of vancomycin on colon tumorigenesis in the context of a standard mouse diet or the TWD.
33803094	5	42	theme	TWD	759:761	arg1	treatments					778:787	both the TWD and vancomycin treatments	750:787	treatments	778:787	While both the TWD and vancomycin treatments independently increased parameters associated with gut inflammation and tumorigenesis compared to AIN93G and plain water controls, mice fed the TWD and treated with vancomycin had significantly increased tumor multiplicity and burden relative to all other treatments.
33803094	1	43	theme	cancer	231:236	arg1	model					187:191	a mouse model	179:191	a mouse model of inflammation-associated colorectal cancer (CAC)	179:242	Previous work by our group using a mouse model of inflammation-associated colorectal cancer (CAC) showed that the total Western diet (TWD) promoted colon tumor development.
33803094	1	44	theme	colon	294:298	arg1	development					306:316	colon tumor development	294:316	colon tumor development	294:316	Previous work by our group using a mouse model of inflammation-associated colorectal cancer (CAC) showed that the total Western diet (TWD) promoted colon tumor development.
33803094	5	45	theme	relative	1023:1030	arg1	multiplicity					999:1010	significantly increased tumor multiplicity	969:1010	significantly increased tumor multiplicity	969:1010	While both the TWD and vancomycin treatments independently increased parameters associated with gut inflammation and tumorigenesis compared to AIN93G and plain water controls, mice fed the TWD and treated with vancomycin had significantly increased tumor multiplicity and burden relative to all other treatments.
33803094	4	46	used	used	617:620	arg2	design					606:611	A 2 × 2 factorial design	588:611	A 2 × 2 factorial design	588:611	A 2 × 2 factorial design was used, in which C57Bl/6J mice were fed either the standard AIN93G diet or TWD and with vancomycin in the drinking water or not.
33803094	5	47	theme	gut	840:842	arg1	inflammation					844:855	gut inflammation	840:855	gut inflammation	840:855	While both the TWD and vancomycin treatments independently increased parameters associated with gut inflammation and tumorigenesis compared to AIN93G and plain water controls, mice fed the TWD and treated with vancomycin had significantly increased tumor multiplicity and burden relative to all other treatments.
33803094	0	48	theme	Vancomycin-Mediated	42:60	arg1	Changes					62:68	Vancomycin-Mediated Changes	42:68	Vancomycin-Mediated Changes to the Gut Microbiome	42:90	The Western Dietary Pattern Combined with Vancomycin-Mediated Changes to the Gut Microbiome Exacerbates Colitis Severity and Colon Tumorigenesis.
33803094	1	49	theme	tumor	300:304	arg1	development					306:316	colon tumor development	294:316	colon tumor development	294:316	Previous work by our group using a mouse model of inflammation-associated colorectal cancer (CAC) showed that the total Western diet (TWD) promoted colon tumor development.
33803094	8	50	theme	gut	1425:1427	arg1	microbiome					1429:1438	the gut microbiome	1421:1438	the gut microbiome	1421:1438	These results support our previous research that the TWD promotes colon tumorigenesis and suggest that vancomycin-induced changes to the gut microbiome are associated with higher tumor rates.
33803094	3	51	theme	standard	556:563	arg1	diet					571:574	a standard mouse diet	554:574	a standard mouse diet	554:574	Therefore, the objective of this study was to determine the impact of vancomycin on colon tumorigenesis in the context of a standard mouse diet or the TWD.
33803094	6	52	from	levels	1193:1198	arg1	abundance					1134:1142	the abundance	1130:1142	the abundance of several taxa at the phylum, family, and genus levels	1130:1198	Vancomycin treatment significantly decreased alpha diversity and changed the abundance of several taxa at the phylum, family, and genus levels.
33803094	3	53	theme	TWD	583:585	arg1	context					543:549	the context	539:549	the context of a standard mouse diet or the TWD	539:585	Therefore, the objective of this study was to determine the impact of vancomycin on colon tumorigenesis in the context of a standard mouse diet or the TWD.
33803094	8	54	theme	previous	1314:1321	arg1	research					1323:1330	our previous research	1310:1330	our previous research	1310:1330	These results support our previous research that the TWD promotes colon tumorigenesis and suggest that vancomycin-induced changes to the gut microbiome are associated with higher tumor rates.
33803094	6	55	theme	Vancomycin	1057:1066	arg1	treatment					1068:1076	Vancomycin treatment	1057:1076	Vancomycin treatment	1057:1076	Vancomycin treatment significantly decreased alpha diversity and changed the abundance of several taxa at the phylum, family, and genus levels.
33803094	5	56	theme	increased	983:991	arg1	multiplicity					999:1010	significantly increased tumor multiplicity	969:1010	significantly increased tumor multiplicity	969:1010	While both the TWD and vancomycin treatments independently increased parameters associated with gut inflammation and tumorigenesis compared to AIN93G and plain water controls, mice fed the TWD and treated with vancomycin had significantly increased tumor multiplicity and burden relative to all other treatments.
33803094	5	57	theme	plain	898:902	arg1	controls					910:917	plain water controls	898:917	plain water controls	898:917	While both the TWD and vancomycin treatments independently increased parameters associated with gut inflammation and tumorigenesis compared to AIN93G and plain water controls, mice fed the TWD and treated with vancomycin had significantly increased tumor multiplicity and burden relative to all other treatments.
33803094	1	58	theme	Previous	146:153	arg1	work					155:158	Previous work	146:158	Previous work by our group using a mouse model of inflammation-associated colorectal cancer (CAC)	146:242	Previous work by our group using a mouse model of inflammation-associated colorectal cancer (CAC) showed that the total Western diet (TWD) promoted colon tumor development.
33803094	4	59	theme	×	592:592	arg1	design					606:611	A 2 × 2 factorial design	588:611	A 2 × 2 factorial design	588:611	A 2 × 2 factorial design was used, in which C57Bl/6J mice were fed either the standard AIN93G diet or TWD and with vancomycin in the drinking water or not.
33803094	4	60	theme	drinking	721:728	arg1	water					730:734	the drinking water	717:734	the drinking water	717:734	A 2 × 2 factorial design was used, in which C57Bl/6J mice were fed either the standard AIN93G diet or TWD and with vancomycin in the drinking water or not.
33803094	3	61	theme	study	465:469	arg1	objective					447:455	the objective	443:455	the objective of this study	443:469	Therefore, the objective of this study was to determine the impact of vancomycin on colon tumorigenesis in the context of a standard mouse diet or the TWD.
33803094	6	62	theme	several	1147:1153	arg1	taxa					1155:1158	several taxa	1147:1158	several taxa	1147:1158	Vancomycin treatment significantly decreased alpha diversity and changed the abundance of several taxa at the phylum, family, and genus levels.
33803094	2	63	theme	vancomycin-mediated	347:365	arg1	changes					367:373	vancomycin-mediated changes	347:373	vancomycin-mediated changes to the gut	347:384	Others have also shown that vancomycin-mediated changes to the gut microbiome increased colorectal cancer (CRC).
32795862	1	0	theme	brown	183:187	arg1	genotypes					197:205	eight brown sorghum genotypes	177:205	eight brown sorghum genotypes	177:205	The phenolic compounds composition, antioxidant and antidiabetic properties of eight brown sorghum genotypes were investigated.
32795862	1	1	dep	compounds	111:119	arg1	compounds					111:119	The phenolic compounds composition, antioxidant and antidiabetic properties	98:172	The phenolic compounds composition, antioxidant and antidiabetic properties of eight brown sorghum genotypes	98:205	The phenolic compounds composition, antioxidant and antidiabetic properties of eight brown sorghum genotypes were investigated.
32795862	1	1	dep	compounds	111:119	arg1	antioxidant					134:144	antioxidant	134:144	antioxidant	134:144	The phenolic compounds composition, antioxidant and antidiabetic properties of eight brown sorghum genotypes were investigated.
32795862	1	1	dep	compounds	111:119	arg1	properties					163:172	antidiabetic properties	150:172	antidiabetic properties	150:172	The phenolic compounds composition, antioxidant and antidiabetic properties of eight brown sorghum genotypes were investigated.
32795862	1	1	dep	compounds	111:119	arg1	composition					121:131	composition	121:131	composition	121:131	The phenolic compounds composition, antioxidant and antidiabetic properties of eight brown sorghum genotypes were investigated.
32795862	7	2	theme	potential	1049:1057	arg1	grains					1038:1043	these sorghum grains	1024:1043	these sorghum grains	1024:1043	Therefore, these sorghum grains are potential candidates for the development of functional foods.
32795862	7	2	theme	potential	1049:1057	arg1	candidates					1059:1068	potential candidates	1049:1068	potential candidates for the development of functional foods	1049:1108	Therefore, these sorghum grains are potential candidates for the development of functional foods.
32795862	2	3	theme	SOR	302:304	arg1	08					306:307	SOR 08	302:307	SOR 08	302:307	DPPH radical scavenging activity was highest in SOR 03, followed by SOR 11, SOR 08 and SOR 33.
32795862	3	4	theme	SOR	337:339	arg1	08					341:342	SOR 08	337:342	SOR 08	337:342	SOR 33, SOR 03, SOR 08, SOR 11 showed the highest ABTS radical scavenging activity.
32795862	3	4	theme	SOR	337:339	arg1	SOR					321:323	SOR 33	321:326	SOR 33	321:326	SOR 33, SOR 03, SOR 08, SOR 11 showed the highest ABTS radical scavenging activity.
32795862	5	5	theme	IC50	781:784	arg1	values					786:791	IC50 values	781:791	IC50 values	781:791	Similarly, SOR 17, SOR 11 and SOR 33 showed significantly potent inhibition of AGEs formation with IC50 values of 14.19, 18.23 and 26.31 µg/ml, respectively, compared to aminoguanidine (AG) (52.30 µg/ml).
32795862	3	6	theme	SOR	329:331	arg1	03					333:334	SOR 03	329:334	SOR 03	329:334	SOR 33, SOR 03, SOR 08, SOR 11 showed the highest ABTS radical scavenging activity.
32795862	3	6	theme	SOR	329:331	arg1	SOR					321:323	SOR 33	321:326	SOR 33	321:326	SOR 33, SOR 03, SOR 08, SOR 11 showed the highest ABTS radical scavenging activity.
32795862	4	7	dep	acarbose	627:634	arg1	27.73 µg/ml					654:664	27.73 µg/ml	654:664	27.73 µg/ml	654:664	Furthermore, SOR 11, SOR 17 and SOR 33 exhibited significantly higher percentage inhibitory activity of α-glucosidase and α-amylase (IC50 = 14.71, 32.98, 24.93 µg/ml and 27.6, 23.84, 45.01 µg/ml, respectively) compared to acarbose (IC50 = 59.34 and 27.73 µg/ml, respectively).
32795862	4	7	dep	acarbose	627:634	arg1	IC50 = 59.34					637:648	IC50 = 59.34	637:648	IC50 = 59.34	637:648	Furthermore, SOR 11, SOR 17 and SOR 33 exhibited significantly higher percentage inhibitory activity of α-glucosidase and α-amylase (IC50 = 14.71, 32.98, 24.93 µg/ml and 27.6, 23.84, 45.01 µg/ml, respectively) compared to acarbose (IC50 = 59.34 and 27.73 µg/ml, respectively).
32795862	1	8	theme	genotypes	197:205	arg1	compounds					111:119	The phenolic compounds composition, antioxidant and antidiabetic properties	98:172	The phenolic compounds composition, antioxidant and antidiabetic properties of eight brown sorghum genotypes	98:205	The phenolic compounds composition, antioxidant and antidiabetic properties of eight brown sorghum genotypes were investigated.
32795862	1	8	theme	genotypes	197:205	arg1	antioxidant					134:144	antioxidant	134:144	antioxidant	134:144	The phenolic compounds composition, antioxidant and antidiabetic properties of eight brown sorghum genotypes were investigated.
32795862	1	8	theme	genotypes	197:205	arg1	composition					121:131	composition	121:131	composition	121:131	The phenolic compounds composition, antioxidant and antidiabetic properties of eight brown sorghum genotypes were investigated.
32795862	4	9	dep	α-glucosidase	509:521	arg1	24.93 µg/ml					559:569	24.93 µg/ml	559:569	24.93 µg/ml	559:569	Furthermore, SOR 11, SOR 17 and SOR 33 exhibited significantly higher percentage inhibitory activity of α-glucosidase and α-amylase (IC50 = 14.71, 32.98, 24.93 µg/ml and 27.6, 23.84, 45.01 µg/ml, respectively) compared to acarbose (IC50 = 59.34 and 27.73 µg/ml, respectively).
32795862	4	9	dep	α-glucosidase	509:521	arg1	45.01 µg/ml					588:598	45.01 µg/ml	588:598	45.01 µg/ml	588:598	Furthermore, SOR 11, SOR 17 and SOR 33 exhibited significantly higher percentage inhibitory activity of α-glucosidase and α-amylase (IC50 = 14.71, 32.98, 24.93 µg/ml and 27.6, 23.84, 45.01 µg/ml, respectively) compared to acarbose (IC50 = 59.34 and 27.73 µg/ml, respectively).
32795862	4	9	dep	α-glucosidase	509:521	arg1	IC50 = 14.71					538:549	IC50 = 14.71	538:549	IC50 = 14.71	538:549	Furthermore, SOR 11, SOR 17 and SOR 33 exhibited significantly higher percentage inhibitory activity of α-glucosidase and α-amylase (IC50 = 14.71, 32.98, 24.93 µg/ml and 27.6, 23.84, 45.01 µg/ml, respectively) compared to acarbose (IC50 = 59.34 and 27.73 µg/ml, respectively).
32795862	4	9	dep	α-glucosidase	509:521	arg1	27.6					575:578	27.6	575:578	27.6	575:578	Furthermore, SOR 11, SOR 17 and SOR 33 exhibited significantly higher percentage inhibitory activity of α-glucosidase and α-amylase (IC50 = 14.71, 32.98, 24.93 µg/ml and 27.6, 23.84, 45.01 µg/ml, respectively) compared to acarbose (IC50 = 59.34 and 27.73 µg/ml, respectively).
32795862	3	10	theme	highest	363:369	arg1	activity					395:402	the highest ABTS radical scavenging activity	359:402	the highest ABTS radical scavenging activity	359:402	SOR 33, SOR 03, SOR 08, SOR 11 showed the highest ABTS radical scavenging activity.
32795862	7	11	theme	functional	1093:1102	arg1	foods					1104:1108	functional foods	1093:1108	functional foods	1093:1108	Therefore, these sorghum grains are potential candidates for the development of functional foods.
32795862	5	12	theme	26.31 µg/ml	813:823	arg1	values					786:791	IC50 values	781:791	IC50 values	781:791	Similarly, SOR 17, SOR 11 and SOR 33 showed significantly potent inhibition of AGEs formation with IC50 values of 14.19, 18.23 and 26.31 µg/ml, respectively, compared to aminoguanidine (AG) (52.30 µg/ml).
32795862	1	13	theme	phenolic	102:109	arg1	compounds					111:119	The phenolic compounds composition, antioxidant and antidiabetic properties	98:172	The phenolic compounds composition, antioxidant and antidiabetic properties of eight brown sorghum genotypes	98:205	The phenolic compounds composition, antioxidant and antidiabetic properties of eight brown sorghum genotypes were investigated.
32795862	1	13	theme	phenolic	102:109	arg1	antioxidant					134:144	antioxidant	134:144	antioxidant	134:144	The phenolic compounds composition, antioxidant and antidiabetic properties of eight brown sorghum genotypes were investigated.
32795862	1	13	theme	phenolic	102:109	arg1	composition					121:131	composition	121:131	composition	121:131	The phenolic compounds composition, antioxidant and antidiabetic properties of eight brown sorghum genotypes were investigated.
32795862	3	14	theme	radical	376:382	arg1	activity					395:402	the highest ABTS radical scavenging activity	359:402	the highest ABTS radical scavenging activity	359:402	SOR 33, SOR 03, SOR 08, SOR 11 showed the highest ABTS radical scavenging activity.
32795862	0	15	theme	UHPLC-ESI-QTOF-MS/MS	0:19	arg1	properties					68:77	antioxidant and antidiabetic properties	39:77	antioxidant and antidiabetic properties of sorghum grains	39:95	UHPLC-ESI-QTOF-MS/MS characterization, antioxidant and antidiabetic properties of sorghum grains.
32795862	0	15	theme	UHPLC-ESI-QTOF-MS/MS	0:19	arg1	characterization					21:36	UHPLC-ESI-QTOF-MS/MS characterization	0:36	UHPLC-ESI-QTOF-MS/MS characterization	0:36	UHPLC-ESI-QTOF-MS/MS characterization, antioxidant and antidiabetic properties of sorghum grains.
32795862	4	16	theme	α-glucosidase	509:521	arg1	activity					497:504	significantly higher percentage inhibitory activity	454:504	significantly higher percentage inhibitory activity of α-glucosidase and α-amylase (IC50 = 14.71, 32.98, 24.93 µg/ml and 27.6, 23.84, 45.01 µg/ml, respectively)	454:613	Furthermore, SOR 11, SOR 17 and SOR 33 exhibited significantly higher percentage inhibitory activity of α-glucosidase and α-amylase (IC50 = 14.71, 32.98, 24.93 µg/ml and 27.6, 23.84, 45.01 µg/ml, respectively) compared to acarbose (IC50 = 59.34 and 27.73 µg/ml, respectively).
32795862	3	17	theme	scavenging	384:393	arg1	activity					395:402	the highest ABTS radical scavenging activity	359:402	the highest ABTS radical scavenging activity	359:402	SOR 33, SOR 03, SOR 08, SOR 11 showed the highest ABTS radical scavenging activity.
32795862	0	18	theme	antioxidant	39:49	arg1	properties					68:77	antioxidant and antidiabetic properties	39:77	antioxidant and antidiabetic properties of sorghum grains	39:95	UHPLC-ESI-QTOF-MS/MS characterization, antioxidant and antidiabetic properties of sorghum grains.
32795862	0	18	theme	antioxidant	39:49	arg1	characterization					21:36	UHPLC-ESI-QTOF-MS/MS characterization	0:36	UHPLC-ESI-QTOF-MS/MS characterization	0:36	UHPLC-ESI-QTOF-MS/MS characterization, antioxidant and antidiabetic properties of sorghum grains.
32795862	5	19	with	formation	766:774	arg1	values					786:791	IC50 values	781:791	IC50 values	781:791	Similarly, SOR 17, SOR 11 and SOR 33 showed significantly potent inhibition of AGEs formation with IC50 values of 14.19, 18.23 and 26.31 µg/ml, respectively, compared to aminoguanidine (AG) (52.30 µg/ml).
32795862	2	20	dep	highest	263:269	arg1	followed					282:289	followed	282:289	followed by SOR 11, SOR 08 and SOR 33	282:318	DPPH radical scavenging activity was highest in SOR 03, followed by SOR 11, SOR 08 and SOR 33.
32795862	4	21	theme	α-amylase	527:535	arg1	activity					497:504	significantly higher percentage inhibitory activity	454:504	significantly higher percentage inhibitory activity of α-glucosidase and α-amylase (IC50 = 14.71, 32.98, 24.93 µg/ml and 27.6, 23.84, 45.01 µg/ml, respectively)	454:613	Furthermore, SOR 11, SOR 17 and SOR 33 exhibited significantly higher percentage inhibitory activity of α-glucosidase and α-amylase (IC50 = 14.71, 32.98, 24.93 µg/ml and 27.6, 23.84, 45.01 µg/ml, respectively) compared to acarbose (IC50 = 59.34 and 27.73 µg/ml, respectively).
32795862	1	22	theme	sorghum	189:195	arg1	genotypes					197:205	eight brown sorghum genotypes	177:205	eight brown sorghum genotypes	177:205	The phenolic compounds composition, antioxidant and antidiabetic properties of eight brown sorghum genotypes were investigated.
32795862	5	23	theme	AGEs	761:764	arg1	formation					766:774	AGEs formation	761:774	AGEs formation with IC50 values of 14.19, 18.23 and 26.31 µg/ml, respectively	761:837	Similarly, SOR 17, SOR 11 and SOR 33 showed significantly potent inhibition of AGEs formation with IC50 values of 14.19, 18.23 and 26.31 µg/ml, respectively, compared to aminoguanidine (AG) (52.30 µg/ml).
32795862	0	24	theme	antidiabetic	55:66	arg1	properties					68:77	antioxidant and antidiabetic properties	39:77	antioxidant and antidiabetic properties of sorghum grains	39:95	UHPLC-ESI-QTOF-MS/MS characterization, antioxidant and antidiabetic properties of sorghum grains.
32795862	0	24	theme	antidiabetic	55:66	arg1	characterization					21:36	UHPLC-ESI-QTOF-MS/MS characterization	0:36	UHPLC-ESI-QTOF-MS/MS characterization	0:36	UHPLC-ESI-QTOF-MS/MS characterization, antioxidant and antidiabetic properties of sorghum grains.
32795862	2	25	theme	SOR	274:276	arg1	03					278:279	SOR 03	274:279	SOR 03	274:279	DPPH radical scavenging activity was highest in SOR 03, followed by SOR 11, SOR 08 and SOR 33.
32795862	5	26	theme	14.19	796:800	arg1	values					786:791	IC50 values	781:791	IC50 values	781:791	Similarly, SOR 17, SOR 11 and SOR 33 showed significantly potent inhibition of AGEs formation with IC50 values of 14.19, 18.23 and 26.31 µg/ml, respectively, compared to aminoguanidine (AG) (52.30 µg/ml).
32795862	5	27	theme	formation	766:774	arg1	inhibition					747:756	significantly potent inhibition	726:756	significantly potent inhibition of AGEs formation with IC50 values of 14.19, 18.23 and 26.31 µg/ml, respectively	726:837	Similarly, SOR 17, SOR 11 and SOR 33 showed significantly potent inhibition of AGEs formation with IC50 values of 14.19, 18.23 and 26.31 µg/ml, respectively, compared to aminoguanidine (AG) (52.30 µg/ml).
32795862	3	28	theme	ABTS	371:374	arg1	activity					395:402	the highest ABTS radical scavenging activity	359:402	the highest ABTS radical scavenging activity	359:402	SOR 33, SOR 03, SOR 08, SOR 11 showed the highest ABTS radical scavenging activity.
32795862	4	29	theme	percentage	475:484	arg1	activity					497:504	significantly higher percentage inhibitory activity	454:504	significantly higher percentage inhibitory activity of α-glucosidase and α-amylase (IC50 = 14.71, 32.98, 24.93 µg/ml and 27.6, 23.84, 45.01 µg/ml, respectively)	454:613	Furthermore, SOR 11, SOR 17 and SOR 33 exhibited significantly higher percentage inhibitory activity of α-glucosidase and α-amylase (IC50 = 14.71, 32.98, 24.93 µg/ml and 27.6, 23.84, 45.01 µg/ml, respectively) compared to acarbose (IC50 = 59.34 and 27.73 µg/ml, respectively).
32795862	5	30	theme	potent	740:745	arg1	inhibition					747:756	significantly potent inhibition	726:756	significantly potent inhibition of AGEs formation with IC50 values of 14.19, 18.23 and 26.31 µg/ml, respectively	726:837	Similarly, SOR 17, SOR 11 and SOR 33 showed significantly potent inhibition of AGEs formation with IC50 values of 14.19, 18.23 and 26.31 µg/ml, respectively, compared to aminoguanidine (AG) (52.30 µg/ml).
32795862	6	31	theme	predominant	939:949	arg1	dihydroflavonols					913:928	dihydroflavonols	913:928	dihydroflavonols	913:928	Flavones, isoflavones and dihydroflavonols were the predominant flavonoids identified in SOR 11, SOR 17 and SOR 33 genotypes.
32795862	6	31	theme	predominant	939:949	arg1	isoflavones					897:907	isoflavones	897:907	isoflavones	897:907	Flavones, isoflavones and dihydroflavonols were the predominant flavonoids identified in SOR 11, SOR 17 and SOR 33 genotypes.
32795862	6	31	theme	predominant	939:949	arg1	flavonoids					951:960	the predominant flavonoids	935:960	the predominant flavonoids identified in SOR 11, SOR 17 and SOR 33 genotypes	935:1010	Flavones, isoflavones and dihydroflavonols were the predominant flavonoids identified in SOR 11, SOR 17 and SOR 33 genotypes.
32795862	6	31	theme	predominant	939:949	arg1	Flavones					887:894	Flavones	887:894	Flavones	887:894	Flavones, isoflavones and dihydroflavonols were the predominant flavonoids identified in SOR 11, SOR 17 and SOR 33 genotypes.
32795862	4	32	theme	higher	468:473	arg1	activity					497:504	significantly higher percentage inhibitory activity	454:504	significantly higher percentage inhibitory activity of α-glucosidase and α-amylase (IC50 = 14.71, 32.98, 24.93 µg/ml and 27.6, 23.84, 45.01 µg/ml, respectively)	454:613	Furthermore, SOR 11, SOR 17 and SOR 33 exhibited significantly higher percentage inhibitory activity of α-glucosidase and α-amylase (IC50 = 14.71, 32.98, 24.93 µg/ml and 27.6, 23.84, 45.01 µg/ml, respectively) compared to acarbose (IC50 = 59.34 and 27.73 µg/ml, respectively).
32795862	7	33	theme	sorghum	1030:1036	arg1	grains					1038:1043	these sorghum grains	1024:1043	these sorghum grains	1024:1043	Therefore, these sorghum grains are potential candidates for the development of functional foods.
32795862	7	33	theme	sorghum	1030:1036	arg1	candidates					1059:1068	potential candidates	1049:1068	potential candidates for the development of functional foods	1049:1108	Therefore, these sorghum grains are potential candidates for the development of functional foods.
32795862	4	34	theme	inhibitory	486:495	arg1	activity					497:504	significantly higher percentage inhibitory activity	454:504	significantly higher percentage inhibitory activity of α-glucosidase and α-amylase (IC50 = 14.71, 32.98, 24.93 µg/ml and 27.6, 23.84, 45.01 µg/ml, respectively)	454:613	Furthermore, SOR 11, SOR 17 and SOR 33 exhibited significantly higher percentage inhibitory activity of α-glucosidase and α-amylase (IC50 = 14.71, 32.98, 24.93 µg/ml and 27.6, 23.84, 45.01 µg/ml, respectively) compared to acarbose (IC50 = 59.34 and 27.73 µg/ml, respectively).
32795862	2	35	theme	scavenging	239:248	arg1	activity					250:257	DPPH radical scavenging activity	226:257	DPPH radical scavenging activity	226:257	DPPH radical scavenging activity was highest in SOR 03, followed by SOR 11, SOR 08 and SOR 33.
32795862	1	36	theme	antidiabetic	150:161	arg1	properties					163:172	antidiabetic properties	150:172	antidiabetic properties	150:172	The phenolic compounds composition, antioxidant and antidiabetic properties of eight brown sorghum genotypes were investigated.
32795862	2	37	theme	radical	231:237	arg1	activity					250:257	DPPH radical scavenging activity	226:257	DPPH radical scavenging activity	226:257	DPPH radical scavenging activity was highest in SOR 03, followed by SOR 11, SOR 08 and SOR 33.
32795862	6	38	theme	SOR	995:997	arg1	genotypes					1002:1010	SOR 33 genotypes	995:1010	SOR 33 genotypes	995:1010	Flavones, isoflavones and dihydroflavonols were the predominant flavonoids identified in SOR 11, SOR 17 and SOR 33 genotypes.
32795862	0	39	theme	grains	90:95	arg1	properties					68:77	antioxidant and antidiabetic properties	39:77	antioxidant and antidiabetic properties of sorghum grains	39:95	UHPLC-ESI-QTOF-MS/MS characterization, antioxidant and antidiabetic properties of sorghum grains.
32795862	0	39	theme	grains	90:95	arg1	characterization					21:36	UHPLC-ESI-QTOF-MS/MS characterization	0:36	UHPLC-ESI-QTOF-MS/MS characterization	0:36	UHPLC-ESI-QTOF-MS/MS characterization, antioxidant and antidiabetic properties of sorghum grains.
32795862	2	40	theme	DPPH	226:229	arg1	activity					250:257	DPPH radical scavenging activity	226:257	DPPH radical scavenging activity	226:257	DPPH radical scavenging activity was highest in SOR 03, followed by SOR 11, SOR 08 and SOR 33.
32795862	0	41	theme	sorghum	82:88	arg1	grains					90:95	sorghum grains	82:95	sorghum grains	82:95	UHPLC-ESI-QTOF-MS/MS characterization, antioxidant and antidiabetic properties of sorghum grains.
32795862	5	42	theme	18.23	803:807	arg1	values					786:791	IC50 values	781:791	IC50 values	781:791	Similarly, SOR 17, SOR 11 and SOR 33 showed significantly potent inhibition of AGEs formation with IC50 values of 14.19, 18.23 and 26.31 µg/ml, respectively, compared to aminoguanidine (AG) (52.30 µg/ml).
32795862	7	43	theme	foods	1104:1108	arg1	development					1078:1088	the development	1074:1088	the development of functional foods	1074:1108	Therefore, these sorghum grains are potential candidates for the development of functional foods.
33518141	10	0	theme	body	2062:2065	arg1	weight					2067:2072	the body weight and average daily gain	2058:2095	weight	2067:2072	The average abundance of the genera Bacteroides and Romboutsia in the cecal digesta was positively correlated with the body weight and average daily gain of broilers in response to LPS challenge.
33518141	3	1	theme	LPS	641:643	arg1	5 mg/kg					594:600	5 mg/kg	594:600	5 mg/kg	594:600	The dietary treatments comprised a basal diet as the control, basal diet plus 5 mg/kg of LPS, and basal diet plus 5 mg/kg of LPS in combination with 1 and 3 g/kg of B. subtilis-fermented products.
33518141	3	1	theme	LPS	641:643	arg1	5 mg/kg					630:636	5 mg/kg	630:636	5 mg/kg	630:636	The dietary treatments comprised a basal diet as the control, basal diet plus 5 mg/kg of LPS, and basal diet plus 5 mg/kg of LPS in combination with 1 and 3 g/kg of B. subtilis-fermented products.
33518141	3	1	theme	LPS	641:643	arg1	diet					557:560	a basal diet	549:560	a basal diet as the control	549:575	The dietary treatments comprised a basal diet as the control, basal diet plus 5 mg/kg of LPS, and basal diet plus 5 mg/kg of LPS in combination with 1 and 3 g/kg of B. subtilis-fermented products.
33518141	3	1	theme	LPS	641:643	arg1	diet					620:623	basal diet	614:623	basal diet	614:623	The dietary treatments comprised a basal diet as the control, basal diet plus 5 mg/kg of LPS, and basal diet plus 5 mg/kg of LPS in combination with 1 and 3 g/kg of B. subtilis-fermented products.
33518141	3	1	theme	LPS	641:643	arg1	diet					584:587	basal diet	578:587	basal diet	578:587	The dietary treatments comprised a basal diet as the control, basal diet plus 5 mg/kg of LPS, and basal diet plus 5 mg/kg of LPS in combination with 1 and 3 g/kg of B. subtilis-fermented products.
33518141	2	2	theme	one-day-old	366:376	arg1	Ross					399:402	Ross 308	399:406	Ross 308	399:406	A total of 120 one-day-old male broiler chicks (Ross 308) were randomly assigned to 4 dietary treatments, with 5 replicate cages per treatment and 6 birds per cage.
33518141	2	2	theme	one-day-old	366:376	arg1	chicks					391:396	120 one-day-old male broiler chicks	362:396	120 one-day-old male broiler chicks (Ross 308)	362:407	A total of 120 one-day-old male broiler chicks (Ross 308) were randomly assigned to 4 dietary treatments, with 5 replicate cages per treatment and 6 birds per cage.
33518141	3	3	with	combination	648:658	arg1	products					703:710	B. subtilis-fermented products	681:710	B. subtilis-fermented products	681:710	The dietary treatments comprised a basal diet as the control, basal diet plus 5 mg/kg of LPS, and basal diet plus 5 mg/kg of LPS in combination with 1 and 3 g/kg of B. subtilis-fermented products.
33518141	3	3	with	combination	648:658	arg1	3 g/kg					671:676	3 g/kg	671:676	3 g/kg	671:676	The dietary treatments comprised a basal diet as the control, basal diet plus 5 mg/kg of LPS, and basal diet plus 5 mg/kg of LPS in combination with 1 and 3 g/kg of B. subtilis-fermented products.
33518141	3	3	with	combination	648:658	arg1	1					665:665	1	665:665	1	665:665	The dietary treatments comprised a basal diet as the control, basal diet plus 5 mg/kg of LPS, and basal diet plus 5 mg/kg of LPS in combination with 1 and 3 g/kg of B. subtilis-fermented products.
33518141	4	4	theme	relative	843:850	arg1	weight					824:829	the body weight	815:829	the body weight of broilers relative to LPS treatment alone	815:873	The results indicated that B. subtilis-fermented product supplementation increased (linear, P < 0.05) the body weight of broilers relative to LPS treatment alone at 21 d of age.
33518141	5	5	dep	improved	973:980	arg1	linear					983:988	linear	983:988	linear	983:988	At 15 to 21 d and 1 to 21 d of age, B. subtilis-fermented product supplementation improved (linear, P < 0.05) the average daily gain in broilers compared with LPS challenge alone.
33518141	5	5	dep	improved	973:980	arg1	P < 0.05					991:998	P < 0.05	991:998	P < 0.05	991:998	At 15 to 21 d and 1 to 21 d of age, B. subtilis-fermented product supplementation improved (linear, P < 0.05) the average daily gain in broilers compared with LPS challenge alone.
33518141	7	6	theme	species	1397:1403	arg1	lower					1409:1413	lower	1409:1413	lower	1409:1413	In cecal microbiota analysis, the richness of bacterial species was lower (P < 0.05) in the groups treated with 1 and 3 g/kg of B. subtilis-fermented products in combination with LPS challenge than in the control group.
33518141	7	6	theme	species	1397:1403	arg1	richness					1375:1382	the richness	1371:1382	the richness of bacterial species	1371:1403	In cecal microbiota analysis, the richness of bacterial species was lower (P < 0.05) in the groups treated with 1 and 3 g/kg of B. subtilis-fermented products in combination with LPS challenge than in the control group.
33518141	7	7	theme	microbiota	1350:1359	arg1	analysis					1361:1368	cecal microbiota analysis	1344:1368	cecal microbiota analysis	1344:1368	In cecal microbiota analysis, the richness of bacterial species was lower (P < 0.05) in the groups treated with 1 and 3 g/kg of B. subtilis-fermented products in combination with LPS challenge than in the control group.
33518141	10	8	theme	daily	2086:2090	arg1	gain					2092:2095	the body weight and average daily gain	2058:2095	gain	2092:2095	The average abundance of the genera Bacteroides and Romboutsia in the cecal digesta was positively correlated with the body weight and average daily gain of broilers in response to LPS challenge.
33518141	3	9	theme	dietary	520:526	arg1	treatments					528:537	The dietary treatments	516:537	The dietary treatments	516:537	The dietary treatments comprised a basal diet as the control, basal diet plus 5 mg/kg of LPS, and basal diet plus 5 mg/kg of LPS in combination with 1 and 3 g/kg of B. subtilis-fermented products.
33518141	1	10	theme	Bacillus	186:193	arg1	products					214:221	Bacillus subtilis-fermented products	186:221	Bacillus subtilis-fermented products	186:221	In this study, the effects of Bacillus subtilis-fermented products on the growth performance and cecal microbiota of broilers were investigated in response to lipopolysaccharide (LPS) challenge.
33518141	4	11	theme	body	819:822	arg1	weight					824:829	the body weight	815:829	the body weight of broilers relative to LPS treatment alone	815:873	The results indicated that B. subtilis-fermented product supplementation increased (linear, P < 0.05) the body weight of broilers relative to LPS treatment alone at 21 d of age.
33518141	6	12	theme	inflammation-associated	1075:1097	arg1	expression					1104:1113	The inflammation-associated gene expression	1071:1113	The inflammation-associated gene expression	1071:1113	The inflammation-associated gene expression was decreased (P < 0.05), and intestinal barrier-associated gene expression was increased (P < 0.05) in the small intestine of the group treated with 3 g/kg of B. subtilis-fermented products in combination with LPS challenge.
33518141	9	13	theme	B.	1879:1880	arg1	products					1901:1908	B. subtilis-fermented products	1879:1908	B. subtilis-fermented products	1879:1908	The abundance of the genera Erysipelatoclostridium and Ruminococcaceae_unclassified in the cecal digesta decreased (P < 0.05) in broilers fed with B. subtilis-fermented products compared with the control group.
33518141	8	14	with	combination	1700:1710	arg1	challenge					1721:1729	LPS challenge	1717:1729	LPS challenge	1717:1729	Principal coordinates analysis indicated distinct clusters between the groups treated with LPS alone and B. subtilis-fermented products in combination with LPS challenge.
33518141	3	15	theme	products	703:710	arg1	products					703:710	B. subtilis-fermented products	681:710	B. subtilis-fermented products	681:710	The dietary treatments comprised a basal diet as the control, basal diet plus 5 mg/kg of LPS, and basal diet plus 5 mg/kg of LPS in combination with 1 and 3 g/kg of B. subtilis-fermented products.
33518141	3	15	theme	products	703:710	arg1	1					665:665	1	665:665	1	665:665	The dietary treatments comprised a basal diet as the control, basal diet plus 5 mg/kg of LPS, and basal diet plus 5 mg/kg of LPS in combination with 1 and 3 g/kg of B. subtilis-fermented products.
33518141	1	16	theme	products	214:221	arg1	effects					175:181	the effects	171:181	the effects of Bacillus subtilis-fermented products on the growth performance and cecal microbiota of broilers	171:280	In this study, the effects of Bacillus subtilis-fermented products on the growth performance and cecal microbiota of broilers were investigated in response to lipopolysaccharide (LPS) challenge.
33518141	12	17	theme	gut	2454:2456	arg1	microflora					2458:2467	gut microflora	2454:2467	the gut microflora composition of broilers	2450:2491	These results demonstrate that B. subtilis-fermented products can improve the growth performance and modulate the gut microflora composition of broilers under immune stress.
33518141	3	18	theme	B.	681:682	arg1	products					703:710	B. subtilis-fermented products	681:710	B. subtilis-fermented products	681:710	The dietary treatments comprised a basal diet as the control, basal diet plus 5 mg/kg of LPS, and basal diet plus 5 mg/kg of LPS in combination with 1 and 3 g/kg of B. subtilis-fermented products.
33518141	3	19	theme	basal	551:555	arg1	diet					557:560	a basal diet	549:560	a basal diet as the control	549:575	The dietary treatments comprised a basal diet as the control, basal diet plus 5 mg/kg of LPS, and basal diet plus 5 mg/kg of LPS in combination with 1 and 3 g/kg of B. subtilis-fermented products.
33518141	8	20	theme	subtilis-fermented	1669:1686	arg1	products					1688:1695	B. subtilis-fermented products	1666:1695	B. subtilis-fermented products	1666:1695	Principal coordinates analysis indicated distinct clusters between the groups treated with LPS alone and B. subtilis-fermented products in combination with LPS challenge.
33518141	4	21	dep	linear	797:802	arg1	P < 0.05					805:812	P < 0.05	805:812	P < 0.05	805:812	The results indicated that B. subtilis-fermented product supplementation increased (linear, P < 0.05) the body weight of broilers relative to LPS treatment alone at 21 d of age.
33518141	7	22	theme	control	1546:1552	arg1	group					1554:1558	the control group	1542:1558	the control group	1542:1558	In cecal microbiota analysis, the richness of bacterial species was lower (P < 0.05) in the groups treated with 1 and 3 g/kg of B. subtilis-fermented products in combination with LPS challenge than in the control group.
33518141	4	23	theme	age	886:888	arg1	21 d					878:881	21 d	878:881	21 d of age	878:888	The results indicated that B. subtilis-fermented product supplementation increased (linear, P < 0.05) the body weight of broilers relative to LPS treatment alone at 21 d of age.
33518141	6	24	theme	gene	1175:1178	arg1	expression					1180:1189	intestinal barrier-associated gene expression	1145:1189	intestinal barrier-associated gene expression	1145:1189	The inflammation-associated gene expression was decreased (P < 0.05), and intestinal barrier-associated gene expression was increased (P < 0.05) in the small intestine of the group treated with 3 g/kg of B. subtilis-fermented products in combination with LPS challenge.
33518141	6	25	dep	decreased	1119:1127	arg1	P < 0.05					1130:1137	P < 0.05	1130:1137	P < 0.05	1130:1137	The inflammation-associated gene expression was decreased (P < 0.05), and intestinal barrier-associated gene expression was increased (P < 0.05) in the small intestine of the group treated with 3 g/kg of B. subtilis-fermented products in combination with LPS challenge.
33518141	6	26	theme	group	1246:1250	arg1	intestine					1229:1237	the small intestine	1219:1237	the small intestine of the group treated with 3 g/kg of B. subtilis-fermented products	1219:1304	The inflammation-associated gene expression was decreased (P < 0.05), and intestinal barrier-associated gene expression was increased (P < 0.05) in the small intestine of the group treated with 3 g/kg of B. subtilis-fermented products in combination with LPS challenge.
33518141	3	27	theme	basal	578:582	arg1	diet					584:587	basal diet	578:587	basal diet	578:587	The dietary treatments comprised a basal diet as the control, basal diet plus 5 mg/kg of LPS, and basal diet plus 5 mg/kg of LPS in combination with 1 and 3 g/kg of B. subtilis-fermented products.
33518141	6	28	with	combination	1309:1319	arg1	challenge					1330:1338	LPS challenge	1326:1338	LPS challenge	1326:1338	The inflammation-associated gene expression was decreased (P < 0.05), and intestinal barrier-associated gene expression was increased (P < 0.05) in the small intestine of the group treated with 3 g/kg of B. subtilis-fermented products in combination with LPS challenge.
33518141	4	29	dep	increased	786:794	arg1	linear					797:802	linear	797:802	linear	797:802	The results indicated that B. subtilis-fermented product supplementation increased (linear, P < 0.05) the body weight of broilers relative to LPS treatment alone at 21 d of age.
33518141	4	30	theme	LPS	855:857	arg1	treatment					859:867	LPS treatment	855:867	LPS treatment alone	855:873	The results indicated that B. subtilis-fermented product supplementation increased (linear, P < 0.05) the body weight of broilers relative to LPS treatment alone at 21 d of age.
33518141	6	31	theme	intestinal	1145:1154	arg1	expression					1180:1189	intestinal barrier-associated gene expression	1145:1189	intestinal barrier-associated gene expression	1145:1189	The inflammation-associated gene expression was decreased (P < 0.05), and intestinal barrier-associated gene expression was increased (P < 0.05) in the small intestine of the group treated with 3 g/kg of B. subtilis-fermented products in combination with LPS challenge.
33518141	9	32	theme	control	1928:1934	arg1	group					1936:1940	the control group	1924:1940	the control group	1924:1940	The abundance of the genera Erysipelatoclostridium and Ruminococcaceae_unclassified in the cecal digesta decreased (P < 0.05) in broilers fed with B. subtilis-fermented products compared with the control group.
33518141	10	33	theme	average	1947:1953	arg1	abundance					1955:1963	The average abundance	1943:1963	The average abundance of the genera Bacteroides and Romboutsia in the cecal digesta	1943:2025	The average abundance of the genera Bacteroides and Romboutsia in the cecal digesta was positively correlated with the body weight and average daily gain of broilers in response to LPS challenge.
33518141	5	34	dep	21 d	900:903	arg1	to					897:898	to	897:898	to	897:898	At 15 to 21 d and 1 to 21 d of age, B. subtilis-fermented product supplementation improved (linear, P < 0.05) the average daily gain in broilers compared with LPS challenge alone.
33518141	6	35	theme	small	1223:1227	arg1	intestine					1229:1237	the small intestine	1219:1237	the small intestine of the group treated with 3 g/kg of B. subtilis-fermented products	1219:1304	The inflammation-associated gene expression was decreased (P < 0.05), and intestinal barrier-associated gene expression was increased (P < 0.05) in the small intestine of the group treated with 3 g/kg of B. subtilis-fermented products in combination with LPS challenge.
33518141	10	36	theme	genera	1972:1977	arg1	abundance					1955:1963	The average abundance	1943:1963	The average abundance of the genera Bacteroides and Romboutsia in the cecal digesta	1943:2025	The average abundance of the genera Bacteroides and Romboutsia in the cecal digesta was positively correlated with the body weight and average daily gain of broilers in response to LPS challenge.
33518141	7	37	with	combination	1503:1513	arg1	challenge					1524:1532	LPS challenge	1520:1532	LPS challenge	1520:1532	In cecal microbiota analysis, the richness of bacterial species was lower (P < 0.05) in the groups treated with 1 and 3 g/kg of B. subtilis-fermented products in combination with LPS challenge than in the control group.
33518141	10	38	theme	cecal	2013:2017	arg1	digesta					2019:2025	the cecal digesta	2009:2025	the cecal digesta	2009:2025	The average abundance of the genera Bacteroides and Romboutsia in the cecal digesta was positively correlated with the body weight and average daily gain of broilers in response to LPS challenge.
33518141	0	39	theme	microbiota	87:96	arg1	community					98:106	cecal microbiota community	81:106	cecal microbiota community in broilers under lipopolysaccharide challenge	81:153	Bacillus subtilis-fermented products ameliorate the growth performance and alter cecal microbiota community in broilers under lipopolysaccharide challenge.
33518141	5	40	theme	LPS	1050:1052	arg1	challenge					1054:1062	LPS challenge	1050:1062	LPS challenge alone	1050:1068	At 15 to 21 d and 1 to 21 d of age, B. subtilis-fermented product supplementation improved (linear, P < 0.05) the average daily gain in broilers compared with LPS challenge alone.
33518141	7	41	theme	subtilis-fermented	1472:1489	arg1	products					1491:1498	B. subtilis-fermented products	1469:1498	B. subtilis-fermented products	1469:1498	In cecal microbiota analysis, the richness of bacterial species was lower (P < 0.05) in the groups treated with 1 and 3 g/kg of B. subtilis-fermented products in combination with LPS challenge than in the control group.
33518141	6	42	theme	subtilis-fermented	1278:1295	arg1	products					1297:1304	B. subtilis-fermented products	1275:1304	B. subtilis-fermented products	1275:1304	The inflammation-associated gene expression was decreased (P < 0.05), and intestinal barrier-associated gene expression was increased (P < 0.05) in the small intestine of the group treated with 3 g/kg of B. subtilis-fermented products in combination with LPS challenge.
33518141	5	43	theme	daily	1013:1017	arg1	gain					1019:1022	the average daily gain	1001:1022	the average daily gain in broilers	1001:1034	At 15 to 21 d and 1 to 21 d of age, B. subtilis-fermented product supplementation improved (linear, P < 0.05) the average daily gain in broilers compared with LPS challenge alone.
33518141	0	44	theme	cecal	81:85	arg1	community					98:106	cecal microbiota community	81:106	cecal microbiota community in broilers under lipopolysaccharide challenge	81:153	Bacillus subtilis-fermented products ameliorate the growth performance and alter cecal microbiota community in broilers under lipopolysaccharide challenge.
33518141	0	45	theme	Bacillus	0:7	arg1	products					28:35	Bacillus subtilis-fermented products	0:35	Bacillus subtilis-fermented products	0:35	Bacillus subtilis-fermented products ameliorate the growth performance and alter cecal microbiota community in broilers under lipopolysaccharide challenge.
33518141	10	46	from	abundance	1955:1963	arg1	digesta					2019:2025	the cecal digesta	2009:2025	the cecal digesta	2009:2025	The average abundance of the genera Bacteroides and Romboutsia in the cecal digesta was positively correlated with the body weight and average daily gain of broilers in response to LPS challenge.
33518141	2	47	theme	dietary	437:443	arg1	treatments					445:454	4 dietary treatments	435:454	4 dietary treatments	435:454	A total of 120 one-day-old male broiler chicks (Ross 308) were randomly assigned to 4 dietary treatments, with 5 replicate cages per treatment and 6 birds per cage.
33518141	10	48	from	weight	2067:2072	arg1	response					2112:2119	response	2112:2119	response to LPS challenge	2112:2136	The average abundance of the genera Bacteroides and Romboutsia in the cecal digesta was positively correlated with the body weight and average daily gain of broilers in response to LPS challenge.
33518141	5	49	from	gain	1019:1022	arg1	broilers					1027:1034	broilers	1027:1034	broilers	1027:1034	At 15 to 21 d and 1 to 21 d of age, B. subtilis-fermented product supplementation improved (linear, P < 0.05) the average daily gain in broilers compared with LPS challenge alone.
33518141	1	50	theme	lipopolysaccharide	315:332	arg1	challenge					340:348	lipopolysaccharide (LPS) challenge	315:348	lipopolysaccharide (LPS) challenge	315:348	In this study, the effects of Bacillus subtilis-fermented products on the growth performance and cecal microbiota of broilers were investigated in response to lipopolysaccharide (LPS) challenge.
33518141	9	51	theme	Erysipelatoclostridium	1760:1781	arg1	abundance					1736:1744	The abundance	1732:1744	The abundance of the genera Erysipelatoclostridium and Ruminococcaceae_unclassified in the cecal digesta	1732:1835	The abundance of the genera Erysipelatoclostridium and Ruminococcaceae_unclassified in the cecal digesta decreased (P < 0.05) in broilers fed with B. subtilis-fermented products compared with the control group.
33518141	12	52	theme	broilers	2484:2491	arg1	composition					2469:2479	the gut microflora composition	2450:2479	the gut microflora composition of broilers	2450:2491	These results demonstrate that B. subtilis-fermented products can improve the growth performance and modulate the gut microflora composition of broilers under immune stress.
33518141	3	53	theme	basal	614:618	arg1	diet					620:623	basal diet	614:623	basal diet	614:623	The dietary treatments comprised a basal diet as the control, basal diet plus 5 mg/kg of LPS, and basal diet plus 5 mg/kg of LPS in combination with 1 and 3 g/kg of B. subtilis-fermented products.
33518141	5	54	theme	subtilis-fermented	930:947	arg1	supplementation					957:971	B. subtilis-fermented product supplementation	927:971	B. subtilis-fermented product supplementation	927:971	At 15 to 21 d and 1 to 21 d of age, B. subtilis-fermented product supplementation improved (linear, P < 0.05) the average daily gain in broilers compared with LPS challenge alone.
33518141	9	55	theme	Ruminococcaceae_unclassified	1787:1814	arg1	abundance					1736:1744	The abundance	1732:1744	The abundance of the genera Erysipelatoclostridium and Ruminococcaceae_unclassified in the cecal digesta	1732:1835	The abundance of the genera Erysipelatoclostridium and Ruminococcaceae_unclassified in the cecal digesta decreased (P < 0.05) in broilers fed with B. subtilis-fermented products compared with the control group.
33518141	10	56	from	gain	2092:2095	arg1	response					2112:2119	response	2112:2119	response to LPS challenge	2112:2136	The average abundance of the genera Bacteroides and Romboutsia in the cecal digesta was positively correlated with the body weight and average daily gain of broilers in response to LPS challenge.
33518141	11	57	theme	B.	2288:2289	arg1	products					2310:2317	B. subtilis-fermented products	2288:2317	B. subtilis-fermented products	2288:2317	Furthermore, the average abundance of the genera Bacteroides and Romboutsia in the cecal digesta was positively correlated with the concentration of B. subtilis-fermented products under LPS challenge.
33518141	4	58	theme	subtilis-fermented	743:760	arg1	supplementation					770:784	B. subtilis-fermented product supplementation	740:784	B. subtilis-fermented product supplementation	740:784	The results indicated that B. subtilis-fermented product supplementation increased (linear, P < 0.05) the body weight of broilers relative to LPS treatment alone at 21 d of age.
33518141	8	59	theme	Principal	1561:1569	arg1	analysis					1583:1590	Principal coordinates analysis	1561:1590	Principal coordinates analysis	1561:1590	Principal coordinates analysis indicated distinct clusters between the groups treated with LPS alone and B. subtilis-fermented products in combination with LPS challenge.
33518141	2	60	theme	chicks	391:396	arg1	total					353:357	A total	351:357	A total of 120 one-day-old male broiler chicks (Ross 308)	351:407	A total of 120 one-day-old male broiler chicks (Ross 308) were randomly assigned to 4 dietary treatments, with 5 replicate cages per treatment and 6 birds per cage.
33518141	11	61	theme	LPS	2325:2327	arg1	challenge					2329:2337	LPS challenge	2325:2337	LPS challenge	2325:2337	Furthermore, the average abundance of the genera Bacteroides and Romboutsia in the cecal digesta was positively correlated with the concentration of B. subtilis-fermented products under LPS challenge.
33518141	12	62	theme	subtilis-fermented	2374:2391	arg1	products					2393:2400	B. subtilis-fermented products	2371:2400	B. subtilis-fermented products	2371:2400	These results demonstrate that B. subtilis-fermented products can improve the growth performance and modulate the gut microflora composition of broilers under immune stress.
33518141	2	63	theme	male	378:381	arg1	Ross					399:402	Ross 308	399:406	Ross 308	399:406	A total of 120 one-day-old male broiler chicks (Ross 308) were randomly assigned to 4 dietary treatments, with 5 replicate cages per treatment and 6 birds per cage.
33518141	2	63	theme	male	378:381	arg1	chicks					391:396	120 one-day-old male broiler chicks	362:396	120 one-day-old male broiler chicks (Ross 308)	362:407	A total of 120 one-day-old male broiler chicks (Ross 308) were randomly assigned to 4 dietary treatments, with 5 replicate cages per treatment and 6 birds per cage.
33518141	8	64	theme	distinct	1602:1609	arg1	clusters					1611:1618	distinct clusters	1602:1618	distinct clusters between the groups treated with LPS alone	1602:1660	Principal coordinates analysis indicated distinct clusters between the groups treated with LPS alone and B. subtilis-fermented products in combination with LPS challenge.
33518141	1	65	theme	broilers	273:280	arg1	microbiota					259:268	cecal microbiota	253:268	cecal microbiota	253:268	In this study, the effects of Bacillus subtilis-fermented products on the growth performance and cecal microbiota of broilers were investigated in response to lipopolysaccharide (LPS) challenge.
33518141	1	65	theme	broilers	273:280	arg1	performance					237:247	growth performance	230:247	growth performance	230:247	In this study, the effects of Bacillus subtilis-fermented products on the growth performance and cecal microbiota of broilers were investigated in response to lipopolysaccharide (LPS) challenge.
33518141	6	66	dep	increased	1195:1203	arg1	P < 0.05					1206:1213	P < 0.05	1206:1213	P < 0.05	1206:1213	The inflammation-associated gene expression was decreased (P < 0.05), and intestinal barrier-associated gene expression was increased (P < 0.05) in the small intestine of the group treated with 3 g/kg of B. subtilis-fermented products in combination with LPS challenge.
33518141	11	67	theme	products	2310:2317	arg1	concentration					2271:2283	the concentration	2267:2283	the concentration of B. subtilis-fermented products under LPS challenge	2267:2337	Furthermore, the average abundance of the genera Bacteroides and Romboutsia in the cecal digesta was positively correlated with the concentration of B. subtilis-fermented products under LPS challenge.
33518141	7	68	theme	bacterial	1387:1395	arg1	species					1397:1403	bacterial species	1387:1403	bacterial species	1387:1403	In cecal microbiota analysis, the richness of bacterial species was lower (P < 0.05) in the groups treated with 1 and 3 g/kg of B. subtilis-fermented products in combination with LPS challenge than in the control group.
33518141	4	69	theme	broilers	834:841	arg1	weight					824:829	the body weight	815:829	the body weight of broilers relative to LPS treatment alone	815:873	The results indicated that B. subtilis-fermented product supplementation increased (linear, P < 0.05) the body weight of broilers relative to LPS treatment alone at 21 d of age.
33518141	12	70	theme	immune	2499:2504	arg1	stress					2506:2511	immune stress	2499:2511	immune stress	2499:2511	These results demonstrate that B. subtilis-fermented products can improve the growth performance and modulate the gut microflora composition of broilers under immune stress.
33518141	11	71	theme	cecal	2222:2226	arg1	digesta					2228:2234	the cecal digesta	2218:2234	the cecal digesta	2218:2234	Furthermore, the average abundance of the genera Bacteroides and Romboutsia in the cecal digesta was positively correlated with the concentration of B. subtilis-fermented products under LPS challenge.
33518141	9	72	from	abundance	1736:1744	arg1	digesta					1829:1835	the cecal digesta	1819:1835	the cecal digesta	1819:1835	The abundance of the genera Erysipelatoclostridium and Ruminococcaceae_unclassified in the cecal digesta decreased (P < 0.05) in broilers fed with B. subtilis-fermented products compared with the control group.
33518141	6	73	theme	gene	1099:1102	arg1	expression					1104:1113	The inflammation-associated gene expression	1071:1113	The inflammation-associated gene expression	1071:1113	The inflammation-associated gene expression was decreased (P < 0.05), and intestinal barrier-associated gene expression was increased (P < 0.05) in the small intestine of the group treated with 3 g/kg of B. subtilis-fermented products in combination with LPS challenge.
33518141	10	74	theme	average	2078:2084	arg1	gain					2092:2095	the body weight and average daily gain	2058:2095	gain	2092:2095	The average abundance of the genera Bacteroides and Romboutsia in the cecal digesta was positively correlated with the body weight and average daily gain of broilers in response to LPS challenge.
33518141	9	75	dep	decreased	1837:1845	arg1	P < 0.05					1848:1855	P < 0.05	1848:1855	P < 0.05	1848:1855	The abundance of the genera Erysipelatoclostridium and Ruminococcaceae_unclassified in the cecal digesta decreased (P < 0.05) in broilers fed with B. subtilis-fermented products compared with the control group.
33518141	1	76	theme	subtilis-fermented	195:212	arg1	products					214:221	Bacillus subtilis-fermented products	186:221	Bacillus subtilis-fermented products	186:221	In this study, the effects of Bacillus subtilis-fermented products on the growth performance and cecal microbiota of broilers were investigated in response to lipopolysaccharide (LPS) challenge.
33518141	7	77	theme	cecal	1344:1348	arg1	analysis					1361:1368	cecal microbiota analysis	1344:1368	cecal microbiota analysis	1344:1368	In cecal microbiota analysis, the richness of bacterial species was lower (P < 0.05) in the groups treated with 1 and 3 g/kg of B. subtilis-fermented products in combination with LPS challenge than in the control group.
33518141	9	78	theme	subtilis-fermented	1882:1899	arg1	products					1901:1908	B. subtilis-fermented products	1879:1908	B. subtilis-fermented products	1879:1908	The abundance of the genera Erysipelatoclostridium and Ruminococcaceae_unclassified in the cecal digesta decreased (P < 0.05) in broilers fed with B. subtilis-fermented products compared with the control group.
33518141	0	79	from	community	98:106	arg1	broilers					111:118	broilers	111:118	broilers under lipopolysaccharide challenge	111:153	Bacillus subtilis-fermented products ameliorate the growth performance and alter cecal microbiota community in broilers under lipopolysaccharide challenge.
33518141	10	80	theme	broilers	2100:2107	arg1	gain					2092:2095	the body weight and average daily gain	2058:2095	gain	2092:2095	The average abundance of the genera Bacteroides and Romboutsia in the cecal digesta was positively correlated with the body weight and average daily gain of broilers in response to LPS challenge.
33518141	10	80	theme	broilers	2100:2107	arg1	weight					2067:2072	the body weight and average daily gain	2058:2095	weight	2067:2072	The average abundance of the genera Bacteroides and Romboutsia in the cecal digesta was positively correlated with the body weight and average daily gain of broilers in response to LPS challenge.
33518141	12	81	theme	microflora	2458:2467	arg1	composition					2469:2479	the gut microflora composition	2450:2479	the gut microflora composition of broilers	2450:2491	These results demonstrate that B. subtilis-fermented products can improve the growth performance and modulate the gut microflora composition of broilers under immune stress.
33518141	3	82	theme	subtilis-fermented	684:701	arg1	products					703:710	B. subtilis-fermented products	681:710	B. subtilis-fermented products	681:710	The dietary treatments comprised a basal diet as the control, basal diet plus 5 mg/kg of LPS, and basal diet plus 5 mg/kg of LPS in combination with 1 and 3 g/kg of B. subtilis-fermented products.
33518141	8	83	theme	B.	1666:1667	arg1	products					1688:1695	B. subtilis-fermented products	1666:1695	B. subtilis-fermented products	1666:1695	Principal coordinates analysis indicated distinct clusters between the groups treated with LPS alone and B. subtilis-fermented products in combination with LPS challenge.
33518141	1	84	theme	growth	230:235	arg1	performance					237:247	growth performance	230:247	growth performance	230:247	In this study, the effects of Bacillus subtilis-fermented products on the growth performance and cecal microbiota of broilers were investigated in response to lipopolysaccharide (LPS) challenge.
33518141	6	85	theme	barrier-associated	1156:1173	arg1	expression					1180:1189	intestinal barrier-associated gene expression	1145:1189	intestinal barrier-associated gene expression	1145:1189	The inflammation-associated gene expression was decreased (P < 0.05), and intestinal barrier-associated gene expression was increased (P < 0.05) in the small intestine of the group treated with 3 g/kg of B. subtilis-fermented products in combination with LPS challenge.
33518141	5	86	theme	average	1005:1011	arg1	gain					1019:1022	the average daily gain	1001:1022	the average daily gain in broilers	1001:1034	At 15 to 21 d and 1 to 21 d of age, B. subtilis-fermented product supplementation improved (linear, P < 0.05) the average daily gain in broilers compared with LPS challenge alone.
33518141	7	87	theme	LPS	1520:1522	arg1	challenge					1524:1532	LPS challenge	1520:1532	LPS challenge	1520:1532	In cecal microbiota analysis, the richness of bacterial species was lower (P < 0.05) in the groups treated with 1 and 3 g/kg of B. subtilis-fermented products in combination with LPS challenge than in the control group.
33518141	10	88	dep	genera	1972:1977	arg1	Bacteroides					1979:1989	Bacteroides	1979:1989	Bacteroides	1979:1989	The average abundance of the genera Bacteroides and Romboutsia in the cecal digesta was positively correlated with the body weight and average daily gain of broilers in response to LPS challenge.
33518141	10	88	dep	genera	1972:1977	arg1	Romboutsia					1995:2004	Romboutsia	1995:2004	Romboutsia	1995:2004	The average abundance of the genera Bacteroides and Romboutsia in the cecal digesta was positively correlated with the body weight and average daily gain of broilers in response to LPS challenge.
33518141	10	89	theme	LPS	2124:2126	arg1	challenge					2128:2136	LPS challenge	2124:2136	LPS challenge	2124:2136	The average abundance of the genera Bacteroides and Romboutsia in the cecal digesta was positively correlated with the body weight and average daily gain of broilers in response to LPS challenge.
33518141	2	90	with	treatments	445:454	arg1	birds					500:504	6 birds	498:504	6 birds per cage	498:513	A total of 120 one-day-old male broiler chicks (Ross 308) were randomly assigned to 4 dietary treatments, with 5 replicate cages per treatment and 6 birds per cage.
33518141	2	90	with	treatments	445:454	arg1	cages					474:478	5 replicate cages	462:478	5 replicate cages per treatment	462:492	A total of 120 one-day-old male broiler chicks (Ross 308) were randomly assigned to 4 dietary treatments, with 5 replicate cages per treatment and 6 birds per cage.
33518141	6	91	theme	LPS	1326:1328	arg1	challenge					1330:1338	LPS challenge	1326:1338	LPS challenge	1326:1338	The inflammation-associated gene expression was decreased (P < 0.05), and intestinal barrier-associated gene expression was increased (P < 0.05) in the small intestine of the group treated with 3 g/kg of B. subtilis-fermented products in combination with LPS challenge.
33518141	8	92	theme	LPS	1717:1719	arg1	challenge					1721:1729	LPS challenge	1717:1729	LPS challenge	1717:1729	Principal coordinates analysis indicated distinct clusters between the groups treated with LPS alone and B. subtilis-fermented products in combination with LPS challenge.
33518141	11	93	theme	subtilis-fermented	2291:2308	arg1	products					2310:2317	B. subtilis-fermented products	2288:2317	B. subtilis-fermented products	2288:2317	Furthermore, the average abundance of the genera Bacteroides and Romboutsia in the cecal digesta was positively correlated with the concentration of B. subtilis-fermented products under LPS challenge.
33518141	7	94	theme	products	1491:1498	arg1	1					1453:1453	1	1453:1453	1	1453:1453	In cecal microbiota analysis, the richness of bacterial species was lower (P < 0.05) in the groups treated with 1 and 3 g/kg of B. subtilis-fermented products in combination with LPS challenge than in the control group.
33518141	7	94	theme	products	1491:1498	arg1	products					1491:1498	B. subtilis-fermented products	1469:1498	B. subtilis-fermented products	1469:1498	In cecal microbiota analysis, the richness of bacterial species was lower (P < 0.05) in the groups treated with 1 and 3 g/kg of B. subtilis-fermented products in combination with LPS challenge than in the control group.
33518141	11	95	theme	genera	2181:2186	arg1	abundance					2164:2172	the average abundance	2152:2172	the average abundance of the genera Bacteroides and Romboutsia in the cecal digesta	2152:2234	Furthermore, the average abundance of the genera Bacteroides and Romboutsia in the cecal digesta was positively correlated with the concentration of B. subtilis-fermented products under LPS challenge.
33518141	7	96	theme	B.	1469:1470	arg1	products					1491:1498	B. subtilis-fermented products	1469:1498	B. subtilis-fermented products	1469:1498	In cecal microbiota analysis, the richness of bacterial species was lower (P < 0.05) in the groups treated with 1 and 3 g/kg of B. subtilis-fermented products in combination with LPS challenge than in the control group.
33518141	6	97	theme	products	1297:1304	arg1	3 g/kg					1265:1270	3 g/kg	1265:1270	3 g/kg of B. subtilis-fermented products	1265:1304	The inflammation-associated gene expression was decreased (P < 0.05), and intestinal barrier-associated gene expression was increased (P < 0.05) in the small intestine of the group treated with 3 g/kg of B. subtilis-fermented products in combination with LPS challenge.
33518141	1	98	dep	performance	237:247	arg1	the					226:228	the	226:228	the	226:228	In this study, the effects of Bacillus subtilis-fermented products on the growth performance and cecal microbiota of broilers were investigated in response to lipopolysaccharide (LPS) challenge.
33518141	2	99	dep	cages	474:478	arg1	replicate					464:472	replicate	464:472	replicate	464:472	A total of 120 one-day-old male broiler chicks (Ross 308) were randomly assigned to 4 dietary treatments, with 5 replicate cages per treatment and 6 birds per cage.
33518141	1	100	from	effects	175:181	arg1	microbiota					259:268	cecal microbiota	253:268	cecal microbiota	253:268	In this study, the effects of Bacillus subtilis-fermented products on the growth performance and cecal microbiota of broilers were investigated in response to lipopolysaccharide (LPS) challenge.
33518141	1	100	from	effects	175:181	arg1	performance					237:247	growth performance	230:247	growth performance	230:247	In this study, the effects of Bacillus subtilis-fermented products on the growth performance and cecal microbiota of broilers were investigated in response to lipopolysaccharide (LPS) challenge.
33518141	6	101	theme	B.	1275:1276	arg1	products					1297:1304	B. subtilis-fermented products	1275:1304	B. subtilis-fermented products	1275:1304	The inflammation-associated gene expression was decreased (P < 0.05), and intestinal barrier-associated gene expression was increased (P < 0.05) in the small intestine of the group treated with 3 g/kg of B. subtilis-fermented products in combination with LPS challenge.
33518141	0	102	theme	lipopolysaccharide	126:143	arg1	challenge					145:153	lipopolysaccharide challenge	126:153	lipopolysaccharide challenge	126:153	Bacillus subtilis-fermented products ameliorate the growth performance and alter cecal microbiota community in broilers under lipopolysaccharide challenge.
33518141	0	103	theme	subtilis-fermented	9:26	arg1	products					28:35	Bacillus subtilis-fermented products	0:35	Bacillus subtilis-fermented products	0:35	Bacillus subtilis-fermented products ameliorate the growth performance and alter cecal microbiota community in broilers under lipopolysaccharide challenge.
33518141	7	104	dep	lower	1409:1413	arg1	P < 0.05					1416:1423	P < 0.05	1416:1423	P < 0.05	1416:1423	In cecal microbiota analysis, the richness of bacterial species was lower (P < 0.05) in the groups treated with 1 and 3 g/kg of B. subtilis-fermented products in combination with LPS challenge than in the control group.
33518141	0	105	theme	growth	52:57	arg1	performance					59:69	the growth performance	48:69	the growth performance	48:69	Bacillus subtilis-fermented products ameliorate the growth performance and alter cecal microbiota community in broilers under lipopolysaccharide challenge.
33518141	5	106	dep	21 d	914:917	arg1	to					911:912	to	911:912	to	911:912	At 15 to 21 d and 1 to 21 d of age, B. subtilis-fermented product supplementation improved (linear, P < 0.05) the average daily gain in broilers compared with LPS challenge alone.
33518141	5	107	theme	B.	927:928	arg1	supplementation					957:971	B. subtilis-fermented product supplementation	927:971	B. subtilis-fermented product supplementation	927:971	At 15 to 21 d and 1 to 21 d of age, B. subtilis-fermented product supplementation improved (linear, P < 0.05) the average daily gain in broilers compared with LPS challenge alone.
33518141	11	108	theme	average	2156:2162	arg1	abundance					2164:2172	the average abundance	2152:2172	the average abundance of the genera Bacteroides and Romboutsia in the cecal digesta	2152:2234	Furthermore, the average abundance of the genera Bacteroides and Romboutsia in the cecal digesta was positively correlated with the concentration of B. subtilis-fermented products under LPS challenge.
33518141	11	109	dep	genera	2181:2186	arg1	Romboutsia					2204:2213	Romboutsia	2204:2213	Romboutsia	2204:2213	Furthermore, the average abundance of the genera Bacteroides and Romboutsia in the cecal digesta was positively correlated with the concentration of B. subtilis-fermented products under LPS challenge.
33518141	11	109	dep	genera	2181:2186	arg1	Bacteroides					2188:2198	Bacteroides	2188:2198	Bacteroides	2188:2198	Furthermore, the average abundance of the genera Bacteroides and Romboutsia in the cecal digesta was positively correlated with the concentration of B. subtilis-fermented products under LPS challenge.
33518141	3	110	theme	LPS	605:607	arg1	5 mg/kg					594:600	5 mg/kg	594:600	5 mg/kg	594:600	The dietary treatments comprised a basal diet as the control, basal diet plus 5 mg/kg of LPS, and basal diet plus 5 mg/kg of LPS in combination with 1 and 3 g/kg of B. subtilis-fermented products.
33518141	3	110	theme	LPS	605:607	arg1	5 mg/kg					630:636	5 mg/kg	630:636	5 mg/kg	630:636	The dietary treatments comprised a basal diet as the control, basal diet plus 5 mg/kg of LPS, and basal diet plus 5 mg/kg of LPS in combination with 1 and 3 g/kg of B. subtilis-fermented products.
33518141	3	110	theme	LPS	605:607	arg1	diet					557:560	a basal diet	549:560	a basal diet as the control	549:575	The dietary treatments comprised a basal diet as the control, basal diet plus 5 mg/kg of LPS, and basal diet plus 5 mg/kg of LPS in combination with 1 and 3 g/kg of B. subtilis-fermented products.
33518141	3	110	theme	LPS	605:607	arg1	diet					620:623	basal diet	614:623	basal diet	614:623	The dietary treatments comprised a basal diet as the control, basal diet plus 5 mg/kg of LPS, and basal diet plus 5 mg/kg of LPS in combination with 1 and 3 g/kg of B. subtilis-fermented products.
33518141	3	110	theme	LPS	605:607	arg1	diet					584:587	basal diet	578:587	basal diet	578:587	The dietary treatments comprised a basal diet as the control, basal diet plus 5 mg/kg of LPS, and basal diet plus 5 mg/kg of LPS in combination with 1 and 3 g/kg of B. subtilis-fermented products.
33518141	5	111	theme	product	949:955	arg1	supplementation					957:971	B. subtilis-fermented product supplementation	927:971	B. subtilis-fermented product supplementation	927:971	At 15 to 21 d and 1 to 21 d of age, B. subtilis-fermented product supplementation improved (linear, P < 0.05) the average daily gain in broilers compared with LPS challenge alone.
33518141	12	112	theme	growth	2418:2423	arg1	performance					2425:2435	the growth performance	2414:2435	the growth performance	2414:2435	These results demonstrate that B. subtilis-fermented products can improve the growth performance and modulate the gut microflora composition of broilers under immune stress.
33518141	11	113	from	abundance	2164:2172	arg1	digesta					2228:2234	the cecal digesta	2218:2234	the cecal digesta	2218:2234	Furthermore, the average abundance of the genera Bacteroides and Romboutsia in the cecal digesta was positively correlated with the concentration of B. subtilis-fermented products under LPS challenge.
33518141	8	114	theme	coordinates	1571:1581	arg1	analysis					1583:1590	Principal coordinates analysis	1561:1590	Principal coordinates analysis	1561:1590	Principal coordinates analysis indicated distinct clusters between the groups treated with LPS alone and B. subtilis-fermented products in combination with LPS challenge.
33518141	9	115	theme	cecal	1823:1827	arg1	digesta					1829:1835	the cecal digesta	1819:1835	the cecal digesta	1819:1835	The abundance of the genera Erysipelatoclostridium and Ruminococcaceae_unclassified in the cecal digesta decreased (P < 0.05) in broilers fed with B. subtilis-fermented products compared with the control group.
33518141	12	116	theme	B.	2371:2372	arg1	products					2393:2400	B. subtilis-fermented products	2371:2400	B. subtilis-fermented products	2371:2400	These results demonstrate that B. subtilis-fermented products can improve the growth performance and modulate the gut microflora composition of broilers under immune stress.
33518141	4	117	theme	B.	740:741	arg1	supplementation					770:784	B. subtilis-fermented product supplementation	740:784	B. subtilis-fermented product supplementation	740:784	The results indicated that B. subtilis-fermented product supplementation increased (linear, P < 0.05) the body weight of broilers relative to LPS treatment alone at 21 d of age.
33518141	1	118	theme	cecal	253:257	arg1	microbiota					259:268	cecal microbiota	253:268	cecal microbiota	253:268	In this study, the effects of Bacillus subtilis-fermented products on the growth performance and cecal microbiota of broilers were investigated in response to lipopolysaccharide (LPS) challenge.
33518141	5	119	theme	age	922:924	arg1	age					922:924	age	922:924	age	922:924	At 15 to 21 d and 1 to 21 d of age, B. subtilis-fermented product supplementation improved (linear, P < 0.05) the average daily gain in broilers compared with LPS challenge alone.
33518141	5	119	theme	age	922:924	arg1	21 d					900:903	21 d	900:903	21 d	900:903	At 15 to 21 d and 1 to 21 d of age, B. subtilis-fermented product supplementation improved (linear, P < 0.05) the average daily gain in broilers compared with LPS challenge alone.
33518141	4	120	theme	product	762:768	arg1	supplementation					770:784	B. subtilis-fermented product supplementation	740:784	B. subtilis-fermented product supplementation	740:784	The results indicated that B. subtilis-fermented product supplementation increased (linear, P < 0.05) the body weight of broilers relative to LPS treatment alone at 21 d of age.
33518141	2	121	theme	broiler	383:389	arg1	Ross					399:402	Ross 308	399:406	Ross 308	399:406	A total of 120 one-day-old male broiler chicks (Ross 308) were randomly assigned to 4 dietary treatments, with 5 replicate cages per treatment and 6 birds per cage.
33518141	2	121	theme	broiler	383:389	arg1	chicks					391:396	120 one-day-old male broiler chicks	362:396	120 one-day-old male broiler chicks (Ross 308)	362:407	A total of 120 one-day-old male broiler chicks (Ross 308) were randomly assigned to 4 dietary treatments, with 5 replicate cages per treatment and 6 birds per cage.
31851606	3	0	from	analysis	255:262	arg1	soil					297:300	saline soil	290:300	saline soil	290:300	During an analysis of bacterial diversity in saline soil, a Gram-stain-negative, aerobic, chitin-degrading bacterial strain, designated SJ-36T, was isolated from saline-alkaline soil sampled at Tumd Right Banner, Inner Mongolia, PR China.
31851606	6	1	dep	based	666:670	arg1	both					725:728	both	725:728	both	725:728	A phylogenetic tree based on 16S rRNA gene sequences and the phylogenomic tree both showed that strain SJ-36T formed a tight clade with Lysobacter maris KMU-14T (sharing 97.6 % 16S rRNA gene similarity) and Lysobacter aestuarii S2-CT (97.8 %).
31851606	11	2	theme	strain	1329:1334	arg1	SJ-36T					1336:1341	strain SJ-36T	1329:1341	strain SJ-36T to L. maris KMU-14T, L. aestuarii S2-CT and other type strains	1329:1404	The average nucleotide identity values of strain SJ-36T to L. maris KMU-14T, L. aestuarii S2-CT and other type strains were 81.5, 79.1 and <79.0 %, respectively.
31851606	8	3	theme	fatty	1076:1080	arg1	iso-C15 					1093:1100	iso-C15 	1093:1100	iso-C15 	1093:1100	The major fatty acids were iso-C15 : 0 (37.5 %), summed feature 9 (14.0 %; iso-C17 : 1ω9c and/or C16 : 0 10-methyl) and iso-C11 : 0 (10.6 %).
31851606	8	3	theme	fatty	1076:1080	arg1	acids					1082:1086	The major fatty acids	1066:1086	The major fatty acids	1066:1086	The major fatty acids were iso-C15 : 0 (37.5 %), summed feature 9 (14.0 %; iso-C17 : 1ω9c and/or C16 : 0 10-methyl) and iso-C11 : 0 (10.6 %).
31851606	12	4	theme	SJ-36T	1562:1567	arg1	discrimination					1537:1550	the discrimination	1533:1550	the discrimination of strain SJ-36T from its phylogenetic relatives	1533:1599	The results of physiological, phenotypic and phylogenetic characterizations allowed the discrimination of strain SJ-36T from its phylogenetic relatives.
31851606	2	5	theme	vital	133:137	arg1	roles					139:143	vital roles	133:143	vital roles	133:143	Strains of Lysobacter, thought to play vital roles in the environment for their high enzyme production capacity, are ubiquitous in various ecosystems.
31851606	14	6	theme	type	1711:1714	arg1	nov.					1628:1631	nov.	1628:1631	nov.	1628:1631	nov. is therefore proposed with strain SJ-36T (=CGMCC 1.16756T=KCTC 43039T) as the type strain.
31851606	14	6	theme	type	1711:1714	arg1	strain					1716:1721	the type strain	1707:1721	the type strain	1707:1721	nov. is therefore proposed with strain SJ-36T (=CGMCC 1.16756T=KCTC 43039T) as the type strain.
31851606	12	7	theme	characterizations	1507:1523	arg1	results					1453:1459	The results	1449:1459	The results of physiological, phenotypic and phylogenetic characterizations	1449:1523	The results of physiological, phenotypic and phylogenetic characterizations allowed the discrimination of strain SJ-36T from its phylogenetic relatives.
31851606	6	8	theme	rRNA	679:682	arg1	sequences					689:697	16S rRNA gene sequences	675:697	16S rRNA gene sequences	675:697	A phylogenetic tree based on 16S rRNA gene sequences and the phylogenomic tree both showed that strain SJ-36T formed a tight clade with Lysobacter maris KMU-14T (sharing 97.6 % 16S rRNA gene similarity) and Lysobacter aestuarii S2-CT (97.8 %).
31851606	3	9	attach	isolated	393:400	arg2	strain					362:367	a Gram-stain-negative, aerobic, chitin-degrading bacterial strain	303:367	a Gram-stain-negative, aerobic, chitin-degrading bacterial strain	303:367	During an analysis of bacterial diversity in saline soil, a Gram-stain-negative, aerobic, chitin-degrading bacterial strain, designated SJ-36T, was isolated from saline-alkaline soil sampled at Tumd Right Banner, Inner Mongolia, PR China.
31851606	3	9	attach	isolated	393:400	arg1	soil					423:426	saline-alkaline soil	407:426	saline-alkaline soil sampled at Tumd Right Banner, Inner Mongolia, PR China	407:481	During an analysis of bacterial diversity in saline soil, a Gram-stain-negative, aerobic, chitin-degrading bacterial strain, designated SJ-36T, was isolated from saline-alkaline soil sampled at Tumd Right Banner, Inner Mongolia, PR China.
31851606	4	10	theme	%	574:574	arg1	optimum					582:588	optimum	582:588	optimum	582:588	Strain SJ-36T grew at 4-40 °C (optimum, 30 °C), pH 5.0-10.0 (optimum, pH 7.0-8.0) and 0-6 % NaCl (optimum, 1.0 %).
31851606	4	10	theme	%	574:574	arg1	 NaCl					575:579	0-6 % NaCl	570:579	0-6 % NaCl (optimum, 1.0 %)	570:596	Strain SJ-36T grew at 4-40 °C (optimum, 30 °C), pH 5.0-10.0 (optimum, pH 7.0-8.0) and 0-6 % NaCl (optimum, 1.0 %).
31851606	7	11	theme	SJ-36T	923:928	arg1	lipids					906:911	The major polar lipids	890:911	The major polar lipids of strain SJ-36T	890:928	The major polar lipids of strain SJ-36T were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, two unidentified lipids and one unidentified phospholipid.
31851606	7	11	theme	SJ-36T	923:928	arg1	phosphatidylethanolamine					935:958	phosphatidylethanolamine	935:958	phosphatidylethanolamine	935:958	The major polar lipids of strain SJ-36T were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, two unidentified lipids and one unidentified phospholipid.
31851606	14	12	theme	=CGMCC	1675:1680	arg1	43039T					1696:1701	=CGMCC 1.16756T=KCTC 43039T	1675:1701	=CGMCC 1.16756T=KCTC 43039T	1675:1701	nov. is therefore proposed with strain SJ-36T (=CGMCC 1.16756T=KCTC 43039T) as the type strain.
31851606	14	12	theme	=CGMCC	1675:1680	arg1	SJ-36T					1667:1672	strain SJ-36T	1660:1672	strain SJ-36T (=CGMCC 1.16756T=KCTC 43039T)	1660:1702	nov. is therefore proposed with strain SJ-36T (=CGMCC 1.16756T=KCTC 43039T) as the type strain.
31851606	6	13	theme	rRNA	827:830	arg1	similarity					837:846	97.6 % 16S rRNA gene similarity	816:846	97.6 % 16S rRNA gene similarity	816:846	A phylogenetic tree based on 16S rRNA gene sequences and the phylogenomic tree both showed that strain SJ-36T formed a tight clade with Lysobacter maris KMU-14T (sharing 97.6 % 16S rRNA gene similarity) and Lysobacter aestuarii S2-CT (97.8 %).
31851606	3	14	theme	saline	290:295	arg1	soil					297:300	saline soil	290:300	saline soil	290:300	During an analysis of bacterial diversity in saline soil, a Gram-stain-negative, aerobic, chitin-degrading bacterial strain, designated SJ-36T, was isolated from saline-alkaline soil sampled at Tumd Right Banner, Inner Mongolia, PR China.
31851606	12	15	theme	phenotypic	1479:1488	arg1	characterizations					1507:1523	physiological, phenotypic and phylogenetic characterizations	1464:1523	physiological, phenotypic and phylogenetic characterizations	1464:1523	The results of physiological, phenotypic and phylogenetic characterizations allowed the discrimination of strain SJ-36T from its phylogenetic relatives.
31851606	6	16	theme	%	821:821	arg1	similarity					837:846	97.6 % 16S rRNA gene similarity	816:846	97.6 % 16S rRNA gene similarity	816:846	A phylogenetic tree based on 16S rRNA gene sequences and the phylogenomic tree both showed that strain SJ-36T formed a tight clade with Lysobacter maris KMU-14T (sharing 97.6 % 16S rRNA gene similarity) and Lysobacter aestuarii S2-CT (97.8 %).
31851606	3	17	theme	diversity	277:285	arg1	analysis					255:262	an analysis	252:262	an analysis of bacterial diversity in saline soil	252:300	During an analysis of bacterial diversity in saline soil, a Gram-stain-negative, aerobic, chitin-degrading bacterial strain, designated SJ-36T, was isolated from saline-alkaline soil sampled at Tumd Right Banner, Inner Mongolia, PR China.
31851606	11	18	theme	average	1291:1297	arg1	identity					1310:1317	The average nucleotide identity	1287:1317	The average nucleotide identity values of strain SJ-36T to L. maris KMU-14T, L. aestuarii S2-CT and other type strains	1287:1404	The average nucleotide identity values of strain SJ-36T to L. maris KMU-14T, L. aestuarii S2-CT and other type strains were 81.5, 79.1 and <79.0 %, respectively.
31851606	7	19	theme	polar	900:904	arg1	lipids					906:911	The major polar lipids	890:911	The major polar lipids of strain SJ-36T	890:928	The major polar lipids of strain SJ-36T were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, two unidentified lipids and one unidentified phospholipid.
31851606	7	19	theme	polar	900:904	arg1	phosphatidylethanolamine					935:958	phosphatidylethanolamine	935:958	phosphatidylethanolamine	935:958	The major polar lipids of strain SJ-36T were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, two unidentified lipids and one unidentified phospholipid.
31851606	12	20	theme	phylogenetic	1494:1505	arg1	characterizations					1507:1523	physiological, phenotypic and phylogenetic characterizations	1464:1523	physiological, phenotypic and phylogenetic characterizations	1464:1523	The results of physiological, phenotypic and phylogenetic characterizations allowed the discrimination of strain SJ-36T from its phylogenetic relatives.
31851606	3	21	theme	Gram-stain-negative	305:323	arg1	strain					362:367	a Gram-stain-negative, aerobic, chitin-degrading bacterial strain	303:367	a Gram-stain-negative, aerobic, chitin-degrading bacterial strain	303:367	During an analysis of bacterial diversity in saline soil, a Gram-stain-negative, aerobic, chitin-degrading bacterial strain, designated SJ-36T, was isolated from saline-alkaline soil sampled at Tumd Right Banner, Inner Mongolia, PR China.
31851606	2	22	theme	various	225:231	arg1	ecosystems					233:242	various ecosystems	225:242	various ecosystems	225:242	Strains of Lysobacter, thought to play vital roles in the environment for their high enzyme production capacity, are ubiquitous in various ecosystems.
31851606	10	23	theme	DNA	1256:1258	arg1	content					1264:1270	Its genomic DNA G+C content	1244:1270	Its genomic DNA G+C content	1244:1270	Its genomic DNA G+C content was 66.6 mol%.
31851606	8	24	theme	 0	1168:1169	arg1	10-methyl					1171:1179	 0 10-methyl	1168:1179	14.0 %; iso-C17 : 1ω9c and/or C16 : 0 10-methyl	1133:1179	The major fatty acids were iso-C15 : 0 (37.5 %), summed feature 9 (14.0 %; iso-C17 : 1ω9c and/or C16 : 0 10-methyl) and iso-C11 : 0 (10.6 %).
31851606	12	25	theme	physiological	1464:1476	arg1	characterizations					1507:1523	physiological, phenotypic and phylogenetic characterizations	1464:1523	physiological, phenotypic and phylogenetic characterizations	1464:1523	The results of physiological, phenotypic and phylogenetic characterizations allowed the discrimination of strain SJ-36T from its phylogenetic relatives.
31851606	11	26	theme	identity	1310:1317	arg1	values					1319:1324	The average nucleotide identity values	1287:1324	The average nucleotide identity values of strain SJ-36T to L. maris KMU-14T, L. aestuarii S2-CT and other type strains	1287:1404	The average nucleotide identity values of strain SJ-36T to L. maris KMU-14T, L. aestuarii S2-CT and other type strains were 81.5, 79.1 and <79.0 %, respectively.
31851606	6	27	theme	aestuarii	864:872	arg1	S2-CT					874:878	aestuarii S2-CT	864:878	aestuarii S2-CT (97.8 %)	864:887	A phylogenetic tree based on 16S rRNA gene sequences and the phylogenomic tree both showed that strain SJ-36T formed a tight clade with Lysobacter maris KMU-14T (sharing 97.6 % 16S rRNA gene similarity) and Lysobacter aestuarii S2-CT (97.8 %).
31851606	6	27	theme	aestuarii	864:872	arg1	%					886:886	97.8 %	881:886	97.8 %	881:886	A phylogenetic tree based on 16S rRNA gene sequences and the phylogenomic tree both showed that strain SJ-36T formed a tight clade with Lysobacter maris KMU-14T (sharing 97.6 % 16S rRNA gene similarity) and Lysobacter aestuarii S2-CT (97.8 %).
31851606	10	28	theme	66.6 mol	1276:1283	arg1	%					1284:1284	66.6 mol%	1276:1284	66.6 mol%	1276:1284	Its genomic DNA G+C content was 66.6 mol%.
31851606	1	29	theme	saline-alkaline	72:86	arg1	soil					88:91	saline-alkaline soil	72:91	saline-alkaline soil	72:91	nov., a chitin-degrading strain isolated from saline-alkaline soil.
31851606	3	30	theme	saline-alkaline	407:421	arg1	soil					423:426	saline-alkaline soil	407:426	saline-alkaline soil sampled at Tumd Right Banner, Inner Mongolia, PR China	407:481	During an analysis of bacterial diversity in saline soil, a Gram-stain-negative, aerobic, chitin-degrading bacterial strain, designated SJ-36T, was isolated from saline-alkaline soil sampled at Tumd Right Banner, Inner Mongolia, PR China.
31851606	0	31	theme	Lysobacter	0:9	arg1	alkalisoli					11:20	Lysobacter alkalisoli	0:20	Lysobacter alkalisoli	0:20	Lysobacter alkalisoli sp.
31851606	2	32	from	ecosystems	233:242	arg1	ubiquitous					211:220	ubiquitous	211:220	ubiquitous	211:220	Strains of Lysobacter, thought to play vital roles in the environment for their high enzyme production capacity, are ubiquitous in various ecosystems.
31851606	6	33	theme	tight	765:769	arg1	clade					771:775	a tight clade	763:775	a tight clade	763:775	A phylogenetic tree based on 16S rRNA gene sequences and the phylogenomic tree both showed that strain SJ-36T formed a tight clade with Lysobacter maris KMU-14T (sharing 97.6 % 16S rRNA gene similarity) and Lysobacter aestuarii S2-CT (97.8 %).
31851606	3	34	dep	Gram-stain-negative	305:323	arg1	chitin-degrading					335:350	chitin-degrading	335:350	chitin-degrading	335:350	During an analysis of bacterial diversity in saline soil, a Gram-stain-negative, aerobic, chitin-degrading bacterial strain, designated SJ-36T, was isolated from saline-alkaline soil sampled at Tumd Right Banner, Inner Mongolia, PR China.
31851606	3	34	dep	Gram-stain-negative	305:323	arg1	aerobic					326:332	aerobic	326:332	aerobic	326:332	During an analysis of bacterial diversity in saline soil, a Gram-stain-negative, aerobic, chitin-degrading bacterial strain, designated SJ-36T, was isolated from saline-alkaline soil sampled at Tumd Right Banner, Inner Mongolia, PR China.
31851606	3	35	theme	Tumd	439:442	arg1	Banner					450:455	Tumd Right Banner	439:455	Tumd Right Banner	439:455	During an analysis of bacterial diversity in saline soil, a Gram-stain-negative, aerobic, chitin-degrading bacterial strain, designated SJ-36T, was isolated from saline-alkaline soil sampled at Tumd Right Banner, Inner Mongolia, PR China.
31851606	3	35	theme	Tumd	439:442	arg1	Mongolia					464:471	Inner Mongolia	458:471	Inner Mongolia	458:471	During an analysis of bacterial diversity in saline soil, a Gram-stain-negative, aerobic, chitin-degrading bacterial strain, designated SJ-36T, was isolated from saline-alkaline soil sampled at Tumd Right Banner, Inner Mongolia, PR China.
31851606	3	35	theme	Tumd	439:442	arg1	China					477:481	PR China	474:481	PR China	474:481	During an analysis of bacterial diversity in saline soil, a Gram-stain-negative, aerobic, chitin-degrading bacterial strain, designated SJ-36T, was isolated from saline-alkaline soil sampled at Tumd Right Banner, Inner Mongolia, PR China.
31851606	11	36	theme	other	1387:1391	arg1	strains					1398:1404	other type strains	1387:1404	other type strains	1387:1404	The average nucleotide identity values of strain SJ-36T to L. maris KMU-14T, L. aestuarii S2-CT and other type strains were 81.5, 79.1 and <79.0 %, respectively.
31851606	6	37	theme	strain	742:747	arg1	SJ-36T					749:754	strain SJ-36T	742:754	strain SJ-36T	742:754	A phylogenetic tree based on 16S rRNA gene sequences and the phylogenomic tree both showed that strain SJ-36T formed a tight clade with Lysobacter maris KMU-14T (sharing 97.6 % 16S rRNA gene similarity) and Lysobacter aestuarii S2-CT (97.8 %).
31851606	2	38	theme	production	186:195	arg1	capacity					197:204	their high enzyme production capacity	168:204	their high enzyme production capacity	168:204	Strains of Lysobacter, thought to play vital roles in the environment for their high enzyme production capacity, are ubiquitous in various ecosystems.
31851606	6	39	theme	phylogenetic	648:659	arg1	tree					661:664	A phylogenetic tree	646:664	A phylogenetic tree	646:664	A phylogenetic tree based on 16S rRNA gene sequences and the phylogenomic tree both showed that strain SJ-36T formed a tight clade with Lysobacter maris KMU-14T (sharing 97.6 % 16S rRNA gene similarity) and Lysobacter aestuarii S2-CT (97.8 %).
31851606	3	40	theme	Inner	458:462	arg1	Banner					450:455	Tumd Right Banner	439:455	Tumd Right Banner	439:455	During an analysis of bacterial diversity in saline soil, a Gram-stain-negative, aerobic, chitin-degrading bacterial strain, designated SJ-36T, was isolated from saline-alkaline soil sampled at Tumd Right Banner, Inner Mongolia, PR China.
31851606	3	40	theme	Inner	458:462	arg1	Mongolia					464:471	Inner Mongolia	458:471	Inner Mongolia	458:471	During an analysis of bacterial diversity in saline soil, a Gram-stain-negative, aerobic, chitin-degrading bacterial strain, designated SJ-36T, was isolated from saline-alkaline soil sampled at Tumd Right Banner, Inner Mongolia, PR China.
31851606	3	41	from	diversity	277:285	arg1	soil					297:300	saline soil	290:300	saline soil	290:300	During an analysis of bacterial diversity in saline soil, a Gram-stain-negative, aerobic, chitin-degrading bacterial strain, designated SJ-36T, was isolated from saline-alkaline soil sampled at Tumd Right Banner, Inner Mongolia, PR China.
31851606	0	42	dep	sp	22:23	arg1	alkalisoli					11:20	Lysobacter alkalisoli	0:20	Lysobacter alkalisoli	0:20	Lysobacter alkalisoli sp.
31851606	12	43	theme	phylogenetic	1578:1589	arg1	relatives					1591:1599	its phylogenetic relatives	1574:1599	its phylogenetic relatives	1574:1599	The results of physiological, phenotypic and phylogenetic characterizations allowed the discrimination of strain SJ-36T from its phylogenetic relatives.
31851606	13	44	theme	Lysobacter	1602:1611	arg1	alkalisoli					1613:1622	Lysobacter alkalisoli	1602:1622	Lysobacter alkalisoli	1602:1622	Lysobacter alkalisoli sp.
31851606	2	45	theme	high	174:177	arg1	capacity					197:204	their high enzyme production capacity	168:204	their high enzyme production capacity	168:204	Strains of Lysobacter, thought to play vital roles in the environment for their high enzyme production capacity, are ubiquitous in various ecosystems.
31851606	11	46	theme	81.5	1411:1414	arg1	%					1432:1432	81.5, 79.1 and <79.0 %	1411:1432	%	1432:1432	The average nucleotide identity values of strain SJ-36T to L. maris KMU-14T, L. aestuarii S2-CT and other type strains were 81.5, 79.1 and <79.0 %, respectively.
31851606	6	47	theme	97.6 	816:820	arg1	%					821:821	%	821:821	%	821:821	A phylogenetic tree based on 16S rRNA gene sequences and the phylogenomic tree both showed that strain SJ-36T formed a tight clade with Lysobacter maris KMU-14T (sharing 97.6 % 16S rRNA gene similarity) and Lysobacter aestuarii S2-CT (97.8 %).
31851606	1	48	theme	chitin-degrading	34:49	arg1	strain					51:56	a chitin-degrading strain	32:56	a chitin-degrading strain	32:56	nov., a chitin-degrading strain isolated from saline-alkaline soil.
31851606	1	48	theme	chitin-degrading	34:49	arg1	nov.					26:29	nov.	26:29	nov.	26:29	nov., a chitin-degrading strain isolated from saline-alkaline soil.
31851606	12	49	from	relatives	1591:1599	arg1	discrimination					1537:1550	the discrimination	1533:1550	the discrimination of strain SJ-36T from its phylogenetic relatives	1533:1599	The results of physiological, phenotypic and phylogenetic characterizations allowed the discrimination of strain SJ-36T from its phylogenetic relatives.
31851606	4	50	theme	pH	554:555	arg1	7.0-8.0					557:563	pH 7.0-8.0	554:563	pH 7.0-8.0	554:563	Strain SJ-36T grew at 4-40 °C (optimum, 30 °C), pH 5.0-10.0 (optimum, pH 7.0-8.0) and 0-6 % NaCl (optimum, 1.0 %).
31851606	11	51	theme	<79.0 	1426:1431	arg1	%					1432:1432	81.5, 79.1 and <79.0 %	1411:1432	%	1432:1432	The average nucleotide identity values of strain SJ-36T to L. maris KMU-14T, L. aestuarii S2-CT and other type strains were 81.5, 79.1 and <79.0 %, respectively.
31851606	7	52	theme	unidentified	1011:1022	arg1	lipids					1024:1029	two unidentified lipids	1007:1029	two unidentified lipids	1007:1029	The major polar lipids of strain SJ-36T were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, two unidentified lipids and one unidentified phospholipid.
31851606	12	53	theme	strain	1555:1560	arg1	SJ-36T					1562:1567	strain SJ-36T	1555:1567	strain SJ-36T	1555:1567	The results of physiological, phenotypic and phylogenetic characterizations allowed the discrimination of strain SJ-36T from its phylogenetic relatives.
31851606	2	54	theme	Lysobacter	105:114	arg1	Strains					94:100	Strains	94:100	Strains of Lysobacter, thought to play vital roles in the environment for their high enzyme production capacity,	94:205	Strains of Lysobacter, thought to play vital roles in the environment for their high enzyme production capacity, are ubiquitous in various ecosystems.
31851606	6	55	theme	phylogenomic	707:718	arg1	tree					720:723	the phylogenomic tree	703:723	the phylogenomic tree	703:723	A phylogenetic tree based on 16S rRNA gene sequences and the phylogenomic tree both showed that strain SJ-36T formed a tight clade with Lysobacter maris KMU-14T (sharing 97.6 % 16S rRNA gene similarity) and Lysobacter aestuarii S2-CT (97.8 %).
31851606	9	56	theme	predominant	1220:1230	arg1	Q-8					1208:1210	Q-8	1208:1210	Q-8	1208:1210	Q-8 was the predominant ubiquinone.
31851606	9	56	theme	predominant	1220:1230	arg1	ubiquinone					1232:1241	the predominant ubiquinone	1216:1241	the predominant ubiquinone	1216:1241	Q-8 was the predominant ubiquinone.
31851606	11	57	theme	SJ-36T	1336:1341	arg1	values					1319:1324	The average nucleotide identity values	1287:1324	The average nucleotide identity values of strain SJ-36T to L. maris KMU-14T, L. aestuarii S2-CT and other type strains	1287:1404	The average nucleotide identity values of strain SJ-36T to L. maris KMU-14T, L. aestuarii S2-CT and other type strains were 81.5, 79.1 and <79.0 %, respectively.
31851606	8	58	theme	major	1070:1074	arg1	iso-C15 					1093:1100	iso-C15 	1093:1100	iso-C15 	1093:1100	The major fatty acids were iso-C15 : 0 (37.5 %), summed feature 9 (14.0 %; iso-C17 : 1ω9c and/or C16 : 0 10-methyl) and iso-C11 : 0 (10.6 %).
31851606	8	58	theme	major	1070:1074	arg1	acids					1082:1086	The major fatty acids	1066:1086	The major fatty acids	1066:1086	The major fatty acids were iso-C15 : 0 (37.5 %), summed feature 9 (14.0 %; iso-C17 : 1ω9c and/or C16 : 0 10-methyl) and iso-C11 : 0 (10.6 %).
31851606	6	59	theme	gene	684:687	arg1	sequences					689:697	16S rRNA gene sequences	675:697	16S rRNA gene sequences	675:697	A phylogenetic tree based on 16S rRNA gene sequences and the phylogenomic tree both showed that strain SJ-36T formed a tight clade with Lysobacter maris KMU-14T (sharing 97.6 % 16S rRNA gene similarity) and Lysobacter aestuarii S2-CT (97.8 %).
31851606	8	60	theme	summed	1115:1120	arg1	feature					1122:1128	summed feature 9	1115:1130	summed feature 9	1115:1130	The major fatty acids were iso-C15 : 0 (37.5 %), summed feature 9 (14.0 %; iso-C17 : 1ω9c and/or C16 : 0 10-methyl) and iso-C11 : 0 (10.6 %).
31851606	8	60	theme	summed	1115:1120	arg1	 0					1102:1103	 0	1102:1103	 0	1102:1103	The major fatty acids were iso-C15 : 0 (37.5 %), summed feature 9 (14.0 %; iso-C17 : 1ω9c and/or C16 : 0 10-methyl) and iso-C11 : 0 (10.6 %).
31851606	5	61	theme	Oxidase	599:605	arg1	activities					620:629	Oxidase and catalase activities	599:629	Oxidase and catalase activities	599:629	Oxidase and catalase activities were positive.
31851606	6	62	theme	16S	675:677	arg1	sequences					689:697	16S rRNA gene sequences	675:697	16S rRNA gene sequences	675:697	A phylogenetic tree based on 16S rRNA gene sequences and the phylogenomic tree both showed that strain SJ-36T formed a tight clade with Lysobacter maris KMU-14T (sharing 97.6 % 16S rRNA gene similarity) and Lysobacter aestuarii S2-CT (97.8 %).
31851606	3	63	theme	bacterial	267:275	arg1	diversity					277:285	bacterial diversity	267:285	bacterial diversity in saline soil	267:300	During an analysis of bacterial diversity in saline soil, a Gram-stain-negative, aerobic, chitin-degrading bacterial strain, designated SJ-36T, was isolated from saline-alkaline soil sampled at Tumd Right Banner, Inner Mongolia, PR China.
31851606	5	64	theme	catalase	611:618	arg1	activities					620:629	Oxidase and catalase activities	599:629	Oxidase and catalase activities	599:629	Oxidase and catalase activities were positive.
31851606	14	65	theme	1.16756T=KCTC	1682:1694	arg1	43039T					1696:1701	=CGMCC 1.16756T=KCTC 43039T	1675:1701	=CGMCC 1.16756T=KCTC 43039T	1675:1701	nov. is therefore proposed with strain SJ-36T (=CGMCC 1.16756T=KCTC 43039T) as the type strain.
31851606	14	65	theme	1.16756T=KCTC	1682:1694	arg1	SJ-36T					1667:1672	strain SJ-36T	1660:1672	strain SJ-36T (=CGMCC 1.16756T=KCTC 43039T)	1660:1702	nov. is therefore proposed with strain SJ-36T (=CGMCC 1.16756T=KCTC 43039T) as the type strain.
31851606	6	66	theme	gene	832:835	arg1	similarity					837:846	97.6 % 16S rRNA gene similarity	816:846	97.6 % 16S rRNA gene similarity	816:846	A phylogenetic tree based on 16S rRNA gene sequences and the phylogenomic tree both showed that strain SJ-36T formed a tight clade with Lysobacter maris KMU-14T (sharing 97.6 % 16S rRNA gene similarity) and Lysobacter aestuarii S2-CT (97.8 %).
31851606	4	67	theme	0-6 	570:573	arg1	%					574:574	%	574:574	%	574:574	Strain SJ-36T grew at 4-40 °C (optimum, 30 °C), pH 5.0-10.0 (optimum, pH 7.0-8.0) and 0-6 % NaCl (optimum, 1.0 %).
31851606	6	68	theme	16S	823:825	arg1	similarity					837:846	97.6 % 16S rRNA gene similarity	816:846	97.6 % 16S rRNA gene similarity	816:846	A phylogenetic tree based on 16S rRNA gene sequences and the phylogenomic tree both showed that strain SJ-36T formed a tight clade with Lysobacter maris KMU-14T (sharing 97.6 % 16S rRNA gene similarity) and Lysobacter aestuarii S2-CT (97.8 %).
31851606	7	69	theme	major	894:898	arg1	lipids					906:911	The major polar lipids	890:911	The major polar lipids of strain SJ-36T	890:928	The major polar lipids of strain SJ-36T were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, two unidentified lipids and one unidentified phospholipid.
31851606	7	69	theme	major	894:898	arg1	phosphatidylethanolamine					935:958	phosphatidylethanolamine	935:958	phosphatidylethanolamine	935:958	The major polar lipids of strain SJ-36T were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, two unidentified lipids and one unidentified phospholipid.
31851606	14	70	theme	strain	1660:1665	arg1	43039T					1696:1701	=CGMCC 1.16756T=KCTC 43039T	1675:1701	=CGMCC 1.16756T=KCTC 43039T	1675:1701	nov. is therefore proposed with strain SJ-36T (=CGMCC 1.16756T=KCTC 43039T) as the type strain.
31851606	14	70	theme	strain	1660:1665	arg1	SJ-36T					1667:1672	strain SJ-36T	1660:1672	strain SJ-36T (=CGMCC 1.16756T=KCTC 43039T)	1660:1702	nov. is therefore proposed with strain SJ-36T (=CGMCC 1.16756T=KCTC 43039T) as the type strain.
31851606	4	71	dep	optimum	545:551	arg1	7.0-8.0					557:563	pH 7.0-8.0	554:563	pH 7.0-8.0	554:563	Strain SJ-36T grew at 4-40 °C (optimum, 30 °C), pH 5.0-10.0 (optimum, pH 7.0-8.0) and 0-6 % NaCl (optimum, 1.0 %).
31851606	10	72	theme	genomic	1248:1254	arg1	content					1264:1270	Its genomic DNA G+C content	1244:1270	Its genomic DNA G+C content	1244:1270	Its genomic DNA G+C content was 66.6 mol%.
31851606	11	73	theme	nucleotide	1299:1308	arg1	identity					1310:1317	The average nucleotide identity	1287:1317	The average nucleotide identity values of strain SJ-36T to L. maris KMU-14T, L. aestuarii S2-CT and other type strains	1287:1404	The average nucleotide identity values of strain SJ-36T to L. maris KMU-14T, L. aestuarii S2-CT and other type strains were 81.5, 79.1 and <79.0 %, respectively.
31851606	4	74	dep	optimum	582:588	arg1	%					595:595	1.0 %	591:595	1.0 %	591:595	Strain SJ-36T grew at 4-40 °C (optimum, 30 °C), pH 5.0-10.0 (optimum, pH 7.0-8.0) and 0-6 % NaCl (optimum, 1.0 %).
31851606	8	75	dep	%	1138:1138	arg1	C16 					1163:1166	C16 	1163:1166	C16 	1163:1166	The major fatty acids were iso-C15 : 0 (37.5 %), summed feature 9 (14.0 %; iso-C17 : 1ω9c and/or C16 : 0 10-methyl) and iso-C11 : 0 (10.6 %).
31851606	8	75	dep	%	1138:1138	arg1	10-methyl					1171:1179	 0 10-methyl	1168:1179	14.0 %; iso-C17 : 1ω9c and/or C16 : 0 10-methyl	1133:1179	The major fatty acids were iso-C15 : 0 (37.5 %), summed feature 9 (14.0 %; iso-C17 : 1ω9c and/or C16 : 0 10-methyl) and iso-C11 : 0 (10.6 %).
31851606	8	75	dep	%	1138:1138	arg1	 1ω9c					1150:1154	 1ω9c	1150:1154	 1ω9c	1150:1154	The major fatty acids were iso-C15 : 0 (37.5 %), summed feature 9 (14.0 %; iso-C17 : 1ω9c and/or C16 : 0 10-methyl) and iso-C11 : 0 (10.6 %).
31851606	8	75	dep	%	1138:1138	arg1	iso-C17 					1141:1148	iso-C17 	1141:1148	iso-C17 	1141:1148	The major fatty acids were iso-C15 : 0 (37.5 %), summed feature 9 (14.0 %; iso-C17 : 1ω9c and/or C16 : 0 10-methyl) and iso-C11 : 0 (10.6 %).
31851606	3	76	theme	bacterial	352:360	arg1	strain					362:367	a Gram-stain-negative, aerobic, chitin-degrading bacterial strain	303:367	a Gram-stain-negative, aerobic, chitin-degrading bacterial strain	303:367	During an analysis of bacterial diversity in saline soil, a Gram-stain-negative, aerobic, chitin-degrading bacterial strain, designated SJ-36T, was isolated from saline-alkaline soil sampled at Tumd Right Banner, Inner Mongolia, PR China.
31851606	4	77	dep	optimum	515:521	arg1	30 °C					524:528	30 °C	524:528	30 °C	524:528	Strain SJ-36T grew at 4-40 °C (optimum, 30 °C), pH 5.0-10.0 (optimum, pH 7.0-8.0) and 0-6 % NaCl (optimum, 1.0 %).
31851606	2	78	from	ubiquitous	211:220	arg1	ecosystems					233:242	various ecosystems	225:242	various ecosystems	225:242	Strains of Lysobacter, thought to play vital roles in the environment for their high enzyme production capacity, are ubiquitous in various ecosystems.
31851606	7	79	theme	strain	916:921	arg1	SJ-36T					923:928	strain SJ-36T	916:928	strain SJ-36T	916:928	The major polar lipids of strain SJ-36T were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, two unidentified lipids and one unidentified phospholipid.
31851606	10	80	theme	G+C	1260:1262	arg1	content					1264:1270	Its genomic DNA G+C content	1244:1270	Its genomic DNA G+C content	1244:1270	Its genomic DNA G+C content was 66.6 mol%.
31851606	13	81	dep	sp	1624:1625	arg1	alkalisoli					1613:1622	Lysobacter alkalisoli	1602:1622	Lysobacter alkalisoli	1602:1622	Lysobacter alkalisoli sp.
31851606	7	82	theme	unidentified	1039:1050	arg1	phospholipid					1052:1063	one unidentified phospholipid	1035:1063	one unidentified phospholipid	1035:1063	The major polar lipids of strain SJ-36T were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, two unidentified lipids and one unidentified phospholipid.
31851606	8	83	dep	 0	1102:1103	arg1	%					1138:1138	14.0 %	1133:1138	14.0 %; iso-C17 : 1ω9c and/or C16 : 0 10-methyl	1133:1179	The major fatty acids were iso-C15 : 0 (37.5 %), summed feature 9 (14.0 %; iso-C17 : 1ω9c and/or C16 : 0 10-methyl) and iso-C11 : 0 (10.6 %).
31851606	3	84	theme	Right	444:448	arg1	Banner					450:455	Tumd Right Banner	439:455	Tumd Right Banner	439:455	During an analysis of bacterial diversity in saline soil, a Gram-stain-negative, aerobic, chitin-degrading bacterial strain, designated SJ-36T, was isolated from saline-alkaline soil sampled at Tumd Right Banner, Inner Mongolia, PR China.
31851606	3	84	theme	Right	444:448	arg1	Mongolia					464:471	Inner Mongolia	458:471	Inner Mongolia	458:471	During an analysis of bacterial diversity in saline soil, a Gram-stain-negative, aerobic, chitin-degrading bacterial strain, designated SJ-36T, was isolated from saline-alkaline soil sampled at Tumd Right Banner, Inner Mongolia, PR China.
31851606	3	84	theme	Right	444:448	arg1	China					477:481	PR China	474:481	PR China	474:481	During an analysis of bacterial diversity in saline soil, a Gram-stain-negative, aerobic, chitin-degrading bacterial strain, designated SJ-36T, was isolated from saline-alkaline soil sampled at Tumd Right Banner, Inner Mongolia, PR China.
31851606	8	85	dep	iso-C15 	1093:1100	arg1	 0					1195:1196	 0	1195:1196	 0	1195:1196	The major fatty acids were iso-C15 : 0 (37.5 %), summed feature 9 (14.0 %; iso-C17 : 1ω9c and/or C16 : 0 10-methyl) and iso-C11 : 0 (10.6 %).
31851606	8	85	dep	iso-C15 	1093:1100	arg1	feature					1122:1128	summed feature 9	1115:1130	summed feature 9	1115:1130	The major fatty acids were iso-C15 : 0 (37.5 %), summed feature 9 (14.0 %; iso-C17 : 1ω9c and/or C16 : 0 10-methyl) and iso-C11 : 0 (10.6 %).
31851606	8	85	dep	iso-C15 	1093:1100	arg1	 0					1102:1103	 0	1102:1103	 0	1102:1103	The major fatty acids were iso-C15 : 0 (37.5 %), summed feature 9 (14.0 %; iso-C17 : 1ω9c and/or C16 : 0 10-methyl) and iso-C11 : 0 (10.6 %).
31851606	8	85	dep	iso-C15 	1093:1100	arg1	%					1111:1111	37.5 %	1106:1111	37.5 %	1106:1111	The major fatty acids were iso-C15 : 0 (37.5 %), summed feature 9 (14.0 %; iso-C17 : 1ω9c and/or C16 : 0 10-methyl) and iso-C11 : 0 (10.6 %).
31851606	8	85	dep	iso-C15 	1093:1100	arg1	%					1204:1204	10.6 %	1199:1204	10.6 %	1199:1204	The major fatty acids were iso-C15 : 0 (37.5 %), summed feature 9 (14.0 %; iso-C17 : 1ω9c and/or C16 : 0 10-methyl) and iso-C11 : 0 (10.6 %).
31851606	8	85	dep	iso-C15 	1093:1100	arg1	iso-C11 					1186:1193	iso-C11 	1186:1193	iso-C11 	1186:1193	The major fatty acids were iso-C15 : 0 (37.5 %), summed feature 9 (14.0 %; iso-C17 : 1ω9c and/or C16 : 0 10-methyl) and iso-C11 : 0 (10.6 %).
31851606	3	86	theme	PR	474:475	arg1	Banner					450:455	Tumd Right Banner	439:455	Tumd Right Banner	439:455	During an analysis of bacterial diversity in saline soil, a Gram-stain-negative, aerobic, chitin-degrading bacterial strain, designated SJ-36T, was isolated from saline-alkaline soil sampled at Tumd Right Banner, Inner Mongolia, PR China.
31851606	3	86	theme	PR	474:475	arg1	China					477:481	PR China	474:481	PR China	474:481	During an analysis of bacterial diversity in saline soil, a Gram-stain-negative, aerobic, chitin-degrading bacterial strain, designated SJ-36T, was isolated from saline-alkaline soil sampled at Tumd Right Banner, Inner Mongolia, PR China.
31851606	2	87	theme	enzyme	179:184	arg1	capacity					197:204	their high enzyme production capacity	168:204	their high enzyme production capacity	168:204	Strains of Lysobacter, thought to play vital roles in the environment for their high enzyme production capacity, are ubiquitous in various ecosystems.
31851606	6	88	dep	maris	793:797	arg1	S2-CT					874:878	aestuarii S2-CT	864:878	aestuarii S2-CT (97.8 %)	864:887	A phylogenetic tree based on 16S rRNA gene sequences and the phylogenomic tree both showed that strain SJ-36T formed a tight clade with Lysobacter maris KMU-14T (sharing 97.6 % 16S rRNA gene similarity) and Lysobacter aestuarii S2-CT (97.8 %).
31851606	6	88	dep	maris	793:797	arg1	%					886:886	97.8 %	881:886	97.8 %	881:886	A phylogenetic tree based on 16S rRNA gene sequences and the phylogenomic tree both showed that strain SJ-36T formed a tight clade with Lysobacter maris KMU-14T (sharing 97.6 % 16S rRNA gene similarity) and Lysobacter aestuarii S2-CT (97.8 %).
31851606	11	89	theme	type	1393:1396	arg1	strains					1398:1404	other type strains	1387:1404	other type strains	1387:1404	The average nucleotide identity values of strain SJ-36T to L. maris KMU-14T, L. aestuarii S2-CT and other type strains were 81.5, 79.1 and <79.0 %, respectively.
31851606	3	90	from	soil	297:300	arg1	analysis					255:262	an analysis	252:262	an analysis of bacterial diversity in saline soil	252:300	During an analysis of bacterial diversity in saline soil, a Gram-stain-negative, aerobic, chitin-degrading bacterial strain, designated SJ-36T, was isolated from saline-alkaline soil sampled at Tumd Right Banner, Inner Mongolia, PR China.
32653771	2	0	theme	acid	558:561	arg1	composition					563:573	the amino acid composition	548:573	the amino acid composition in rat plasma and urine	548:597	Therefore, we hypothesized that a diet with high content of fructose would affect the amino acid composition in rat plasma and urine differently from a diet with high sucrose content.
32653771	3	1	theme	free	900:903	arg1	urine					928:932	urine	928:932	urine	928:932	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	3	1	theme	free	900:903	arg1	plasma					917:922	plasma	917:922	plasma	917:922	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	3	1	theme	free	900:903	arg1	acids					911:915	individual free amino acids plasma and urine	889:932	individual free amino acids plasma and urine	889:932	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	5	2	theme	acid	1218:1221	arg1	concentrations					1270:1283	higher urine concentrations	1257:1283	higher urine concentrations of arginine and citrulline	1257:1310	Rats fed high-fructose high-fat diet had higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine and higher urine concentrations of arginine and citrulline when compared to rats fed high-sucrose high-fat diet.
32653771	5	2	theme	acid	1218:1221	arg1	concentrations					1167:1180	higher plasma concentrations	1153:1180	higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine	1153:1251	Rats fed high-fructose high-fat diet had higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine and higher urine concentrations of arginine and citrulline when compared to rats fed high-sucrose high-fat diet.
32653771	1	3	theme	fructose	243:250	arg1	intake					252:257	High fructose intake	238:257	High fructose intake	238:257	High fructose intake has been shown to increase circulating alanine transaminase in humans, which could reflect damage to the liver by fructose but could also be linked to higher level of transamination of amino acids in liver.
32653771	7	4	theme	C	1591:1591	arg1	concentrations					1627:1640	urine cystatin C and T cell immunoglobulin mucin-1 concentrations	1576:1640	urine cystatin C and T cell immunoglobulin mucin-1 concentrations	1576:1640	Serum concentrations of alanine transaminase, aspartate transaminase, and creatinine, and urine cystatin C and T cell immunoglobulin mucin-1 concentrations were comparable between the groups and within normal ranges.
32653771	7	5	theme	T	1597:1597	arg1	mucin-1					1619:1625	T cell immunoglobulin mucin-1	1597:1625	T cell immunoglobulin mucin-1	1597:1625	Serum concentrations of alanine transaminase, aspartate transaminase, and creatinine, and urine cystatin C and T cell immunoglobulin mucin-1 concentrations were comparable between the groups and within normal ranges.
32653771	8	6	from	sucrose	1739:1745	arg1	diets					1783:1787	high-fat diets	1774:1787	high-fat diets	1774:1787	To conclude, substituting high-dose sucrose with high-dose fructose in high-fat diets affected amino acid compositions in plasma and urine.
32653771	5	7	theme	ornithine	1224:1232	arg1	concentrations					1270:1283	higher urine concentrations	1257:1283	higher urine concentrations of arginine and citrulline	1257:1310	Rats fed high-fructose high-fat diet had higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine and higher urine concentrations of arginine and citrulline when compared to rats fed high-sucrose high-fat diet.
32653771	5	7	theme	ornithine	1224:1232	arg1	concentrations					1167:1180	higher plasma concentrations	1153:1180	higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine	1153:1251	Rats fed high-fructose high-fat diet had higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine and higher urine concentrations of arginine and citrulline when compared to rats fed high-sucrose high-fat diet.
32653771	7	8	theme	urine	1576:1580	arg1	C					1591:1591	urine cystatin C	1576:1591	urine cystatin C	1576:1591	Serum concentrations of alanine transaminase, aspartate transaminase, and creatinine, and urine cystatin C and T cell immunoglobulin mucin-1 concentrations were comparable between the groups and within normal ranges.
32653771	3	9	theme	high	658:661	arg1	intake					663:668	high intake	658:668	high intake of sucrose and fructose	658:692	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	0	10	theme	higher	182:187	arg1	concentrations					195:208	higher urine concentrations	182:208	higher urine concentrations of arginine and citrulline	182:235	Substitution of high-dose sucrose with fructose in high-fat diets resulted in higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline.
32653771	1	11	theme	transamination	426:439	arg1	level					417:421	higher level	410:421	higher level of transamination of amino acids in liver	410:463	High fructose intake has been shown to increase circulating alanine transaminase in humans, which could reflect damage to the liver by fructose but could also be linked to higher level of transamination of amino acids in liver.
32653771	8	12	theme	high-dose	1729:1737	arg1	sucrose					1739:1745	substituting high-dose sucrose	1716:1745	substituting high-dose sucrose with high-dose fructose in high-fat diets	1716:1787	To conclude, substituting high-dose sucrose with high-dose fructose in high-fat diets affected amino acid compositions in plasma and urine.
32653771	0	13	theme	concentrations	195:208	arg1	concentrations					92:105	higher plasma concentrations	78:105	higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline	78:235	Substitution of high-dose sucrose with fructose in high-fat diets resulted in higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline.
32653771	1	14	theme	amino	444:448	arg1	acids					450:454	amino acids	444:454	amino acids in liver	444:463	High fructose intake has been shown to increase circulating alanine transaminase in humans, which could reflect damage to the liver by fructose but could also be linked to higher level of transamination of amino acids in liver.
32653771	2	15	theme	high	510:513	arg1	content					515:521	high content	510:521	high content of fructose	510:533	Therefore, we hypothesized that a diet with high content of fructose would affect the amino acid composition in rat plasma and urine differently from a diet with high sucrose content.
32653771	0	16	theme	acid	119:122	arg1	concentrations					92:105	higher plasma concentrations	78:105	higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline	78:235	Substitution of high-dose sucrose with fructose in high-fat diets resulted in higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline.
32653771	4	17	theme	high	991:994	arg1	content					1005:1011	normal (10 wt%) or high (40 wt%) content	972:1011	normal (10 wt%) or high (40 wt%) content of sucrose or fructose	972:1034	Male Wistar rats were fed diets with normal (10 wt%) or high (40 wt%) content of sucrose or fructose, with normal or high fat content (7 or 22 wt%) and 20 wt% protein (casein).
32653771	3	18	theme	high	720:723	arg1	intake					725:730	high intake	720:730	high intake of saturated fat in the Western-style diet	720:773	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	0	19	theme	cystine	125:131	arg1	concentrations					92:105	higher plasma concentrations	78:105	higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline	78:235	Substitution of high-dose sucrose with fructose in high-fat diets resulted in higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline.
32653771	6	20	theme	sucrose	1397:1403	arg1	content					1386:1392	Substituting normal content	1366:1392	Substituting normal content of sucrose with fructose in the diets	1366:1430	Substituting normal content of sucrose with fructose in the diets had little impact on amino acids in plasma and urine.
32653771	4	21	theme	fructose	1027:1034	arg1	content					1005:1011	normal (10 wt%) or high (40 wt%) content	972:1011	normal (10 wt%) or high (40 wt%) content of sucrose or fructose	972:1034	Male Wistar rats were fed diets with normal (10 wt%) or high (40 wt%) content of sucrose or fructose, with normal or high fat content (7 or 22 wt%) and 20 wt% protein (casein).
32653771	0	22	theme	glutamic	134:141	arg1	acid					143:146	glutamic acid	134:146	glutamic acid	134:146	Substitution of high-dose sucrose with fructose in high-fat diets resulted in higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline.
32653771	7	23	theme	aspartate	1532:1540	arg1	transaminase					1542:1553	aspartate transaminase	1532:1553	aspartate transaminase	1532:1553	Serum concentrations of alanine transaminase, aspartate transaminase, and creatinine, and urine cystatin C and T cell immunoglobulin mucin-1 concentrations were comparable between the groups and within normal ranges.
32653771	3	24	from	fructose/sucrose	817:832	arg1	diets					837:841	diets	837:841	diets with normal or high content of coconut oil	837:884	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	2	25	theme	sucrose	633:639	arg1	content					641:647	high sucrose content	628:647	high sucrose content	628:647	Therefore, we hypothesized that a diet with high content of fructose would affect the amino acid composition in rat plasma and urine differently from a diet with high sucrose content.
32653771	3	26	theme	fat	745:747	arg1	intake					725:730	high intake	720:730	high intake of saturated fat in the Western-style diet	720:773	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	6	27	theme	normal	1379:1384	arg1	content					1386:1392	Substituting normal content	1366:1392	Substituting normal content of sucrose with fructose in the diets	1366:1430	Substituting normal content of sucrose with fructose in the diets had little impact on amino acids in plasma and urine.
32653771	3	28	with	diets	837:841	arg1	content					863:869	normal or high content	848:869	normal or high content of coconut oil	848:884	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	6	29	theme	Substituting	1366:1377	arg1	content					1386:1392	Substituting normal content	1366:1392	Substituting normal content of sucrose with fructose in the diets	1366:1430	Substituting normal content of sucrose with fructose in the diets had little impact on amino acids in plasma and urine.
32653771	7	30	theme	transaminase	1518:1529	arg1	concentrations					1627:1640	urine cystatin C and T cell immunoglobulin mucin-1 concentrations	1576:1640	urine cystatin C and T cell immunoglobulin mucin-1 concentrations	1576:1640	Serum concentrations of alanine transaminase, aspartate transaminase, and creatinine, and urine cystatin C and T cell immunoglobulin mucin-1 concentrations were comparable between the groups and within normal ranges.
32653771	7	30	theme	transaminase	1518:1529	arg1	concentrations					1492:1505	Serum concentrations	1486:1505	Serum concentrations of alanine transaminase, aspartate transaminase, and creatinine	1486:1569	Serum concentrations of alanine transaminase, aspartate transaminase, and creatinine, and urine cystatin C and T cell immunoglobulin mucin-1 concentrations were comparable between the groups and within normal ranges.
32653771	3	31	from	diets	837:841	arg1	effects					801:807	the effects	797:807	the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine	797:932	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	5	32	theme	urine	1264:1268	arg1	concentrations					1270:1283	higher urine concentrations	1257:1283	higher urine concentrations of arginine and citrulline	1257:1310	Rats fed high-fructose high-fat diet had higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine and higher urine concentrations of arginine and citrulline when compared to rats fed high-sucrose high-fat diet.
32653771	4	33	theme	sucrose	1016:1022	arg1	content					1005:1011	normal (10 wt%) or high (40 wt%) content	972:1011	normal (10 wt%) or high (40 wt%) content of sucrose or fructose	972:1034	Male Wistar rats were fed diets with normal (10 wt%) or high (40 wt%) content of sucrose or fructose, with normal or high fat content (7 or 22 wt%) and 20 wt% protein (casein).
32653771	8	34	theme	high-fat	1774:1781	arg1	diets					1783:1787	high-fat diets	1774:1787	high-fat diets	1774:1787	To conclude, substituting high-dose sucrose with high-dose fructose in high-fat diets affected amino acid compositions in plasma and urine.
32653771	4	35	dep	normal	972:977	arg1	%					985:985	10 wt%	980:985	10 wt%	980:985	Male Wistar rats were fed diets with normal (10 wt%) or high (40 wt%) content of sucrose or fructose, with normal or high fat content (7 or 22 wt%) and 20 wt% protein (casein).
32653771	7	36	theme	Serum	1486:1490	arg1	concentrations					1492:1505	Serum concentrations	1486:1505	Serum concentrations of alanine transaminase, aspartate transaminase, and creatinine	1486:1569	Serum concentrations of alanine transaminase, aspartate transaminase, and creatinine, and urine cystatin C and T cell immunoglobulin mucin-1 concentrations were comparable between the groups and within normal ranges.
32653771	0	37	from	Substitution	0:11	arg1	diets					60:64	high-fat diets	51:64	high-fat diets	51:64	Substitution of high-dose sucrose with fructose in high-fat diets resulted in higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline.
32653771	0	38	theme	plasma	85:90	arg1	concentrations					92:105	higher plasma concentrations	78:105	higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline	78:235	Substitution of high-dose sucrose with fructose in high-fat diets resulted in higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline.
32653771	8	39	theme	acid	1804:1807	arg1	compositions					1809:1820	amino acid compositions	1798:1820	amino acid compositions in plasma and urine	1798:1840	To conclude, substituting high-dose sucrose with high-dose fructose in high-fat diets affected amino acid compositions in plasma and urine.
32653771	3	40	theme	fructose/sucrose	817:832	arg1	effects					801:807	the effects	797:807	the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine	797:932	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	4	41	theme	Wistar	940:945	arg1	rats					947:950	Male Wistar rats	935:950	Male Wistar rats	935:950	Male Wistar rats were fed diets with normal (10 wt%) or high (40 wt%) content of sucrose or fructose, with normal or high fat content (7 or 22 wt%) and 20 wt% protein (casein).
32653771	5	42	theme	high-fat	1135:1142	arg1	diet					1144:1147	high-fructose high-fat diet	1121:1147	high-fructose high-fat diet	1121:1147	Rats fed high-fructose high-fat diet had higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine and higher urine concentrations of arginine and citrulline when compared to rats fed high-sucrose high-fat diet.
32653771	3	43	theme	high	858:861	arg1	content					863:869	normal or high content	848:869	normal or high content of coconut oil	848:884	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	4	44	theme	fat	1057:1059	arg1	content					1061:1067	normal or high fat content	1042:1067	content	1061:1067	Male Wistar rats were fed diets with normal (10 wt%) or high (40 wt%) content of sucrose or fructose, with normal or high fat content (7 or 22 wt%) and 20 wt% protein (casein).
32653771	4	44	theme	fat	1057:1059	arg1	%					1080:1080	7 or 22 wt%	1070:1080	7 or 22 wt%	1070:1080	Male Wistar rats were fed diets with normal (10 wt%) or high (40 wt%) content of sucrose or fructose, with normal or high fat content (7 or 22 wt%) and 20 wt% protein (casein).
32653771	0	45	theme	high-dose	16:24	arg1	sucrose					26:32	high-dose sucrose	16:32	high-dose sucrose	16:32	Substitution of high-dose sucrose with fructose in high-fat diets resulted in higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline.
32653771	3	46	theme	normal	848:853	arg1	content					863:869	normal or high content	848:869	normal or high content of coconut oil	848:884	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	1	47	attach	linked	400:405	arg1	level					417:421	higher level	410:421	higher level of transamination of amino acids in liver	410:463	High fructose intake has been shown to increase circulating alanine transaminase in humans, which could reflect damage to the liver by fructose but could also be linked to higher level of transamination of amino acids in liver.
32653771	1	47	attach	linked	400:405	arg2	transaminase					306:317	circulating alanine transaminase	286:317	circulating alanine transaminase	286:317	High fructose intake has been shown to increase circulating alanine transaminase in humans, which could reflect damage to the liver by fructose but could also be linked to higher level of transamination of amino acids in liver.
32653771	1	48	theme	circulating	286:296	arg1	transaminase					306:317	circulating alanine transaminase	286:317	circulating alanine transaminase	286:317	High fructose intake has been shown to increase circulating alanine transaminase in humans, which could reflect damage to the liver by fructose but could also be linked to higher level of transamination of amino acids in liver.
32653771	7	49	theme	normal	1688:1693	arg1	ranges					1695:1700	normal ranges	1688:1700	normal ranges	1688:1700	Serum concentrations of alanine transaminase, aspartate transaminase, and creatinine, and urine cystatin C and T cell immunoglobulin mucin-1 concentrations were comparable between the groups and within normal ranges.
32653771	3	50	theme	oil	882:884	arg1	content					863:869	normal or high content	848:869	normal or high content of coconut oil	848:884	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	1	51	from	liver	459:463	arg1	transamination					426:439	transamination	426:439	transamination of amino acids in liver	426:463	High fructose intake has been shown to increase circulating alanine transaminase in humans, which could reflect damage to the liver by fructose but could also be linked to higher level of transamination of amino acids in liver.
32653771	2	52	from	composition	563:573	arg1	urine					593:597	urine	593:597	urine	593:597	Therefore, we hypothesized that a diet with high content of fructose would affect the amino acid composition in rat plasma and urine differently from a diet with high sucrose content.
32653771	2	52	from	composition	563:573	arg1	plasma					582:587	rat plasma	578:587	rat plasma	578:587	Therefore, we hypothesized that a diet with high content of fructose would affect the amino acid composition in rat plasma and urine differently from a diet with high sucrose content.
32653771	4	53	theme	%	1092:1092	arg1	casein					1103:1108	20 wt% protein (casein)	1087:1109	20 wt% protein (casein)	1087:1109	Male Wistar rats were fed diets with normal (10 wt%) or high (40 wt%) content of sucrose or fructose, with normal or high fat content (7 or 22 wt%) and 20 wt% protein (casein).
32653771	7	54	theme	mucin-1	1619:1625	arg1	concentrations					1627:1640	urine cystatin C and T cell immunoglobulin mucin-1 concentrations	1576:1640	urine cystatin C and T cell immunoglobulin mucin-1 concentrations	1576:1640	Serum concentrations of alanine transaminase, aspartate transaminase, and creatinine, and urine cystatin C and T cell immunoglobulin mucin-1 concentrations were comparable between the groups and within normal ranges.
32653771	1	55	from	acids	450:454	arg1	liver					459:463	liver	459:463	liver	459:463	High fructose intake has been shown to increase circulating alanine transaminase in humans, which could reflect damage to the liver by fructose but could also be linked to higher level of transamination of amino acids in liver.
32653771	0	56	with	Substitution	0:11	arg1	fructose					39:46	fructose	39:46	fructose	39:46	Substitution of high-dose sucrose with fructose in high-fat diets resulted in higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline.
32653771	3	57	theme	amino	905:909	arg1	urine					928:932	urine	928:932	urine	928:932	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	3	57	theme	amino	905:909	arg1	plasma					917:922	plasma	917:922	plasma	917:922	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	3	57	theme	amino	905:909	arg1	acids					911:915	individual free amino acids plasma and urine	889:932	individual free amino acids plasma and urine	889:932	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	6	58	contain	had	1432:1434	arg2	impact					1443:1448	little impact	1436:1448	little impact	1436:1448	Substituting normal content of sucrose with fructose in the diets had little impact on amino acids in plasma and urine.
32653771	6	58	contain	had	1432:1434	arg1	content					1386:1392	Substituting normal content	1366:1392	Substituting normal content of sucrose with fructose in the diets	1366:1430	Substituting normal content of sucrose with fructose in the diets had little impact on amino acids in plasma and urine.
32653771	3	59	theme	individual	889:898	arg1	urine					928:932	urine	928:932	urine	928:932	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	3	59	theme	individual	889:898	arg1	plasma					917:922	plasma	917:922	plasma	917:922	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	3	59	theme	individual	889:898	arg1	acids					911:915	individual free amino acids plasma and urine	889:932	individual free amino acids plasma and urine	889:932	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	1	60	from	transaminase	306:317	arg1	humans					322:327	humans	322:327	humans	322:327	High fructose intake has been shown to increase circulating alanine transaminase in humans, which could reflect damage to the liver by fructose but could also be linked to higher level of transamination of amino acids in liver.
32653771	5	61	theme	plasma	1160:1165	arg1	concentrations					1167:1180	higher plasma concentrations	1153:1180	higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine	1153:1251	Rats fed high-fructose high-fat diet had higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine and higher urine concentrations of arginine and citrulline when compared to rats fed high-sucrose high-fat diet.
32653771	5	62	contain	had	1149:1151	arg2	concentrations					1270:1283	higher urine concentrations	1257:1283	higher urine concentrations of arginine and citrulline	1257:1310	Rats fed high-fructose high-fat diet had higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine and higher urine concentrations of arginine and citrulline when compared to rats fed high-sucrose high-fat diet.
32653771	5	62	contain	had	1149:1151	arg2	concentrations					1167:1180	higher plasma concentrations	1153:1180	higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine	1153:1251	Rats fed high-fructose high-fat diet had higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine and higher urine concentrations of arginine and citrulline when compared to rats fed high-sucrose high-fat diet.
32653771	5	62	contain	had	1149:1151	arg1	Rats					1112:1115	Rats	1112:1115	Rats fed high-fructose high-fat diet	1112:1147	Rats fed high-fructose high-fat diet had higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine and higher urine concentrations of arginine and citrulline when compared to rats fed high-sucrose high-fat diet.
32653771	1	63	theme	High	238:241	arg1	intake					252:257	High fructose intake	238:257	High fructose intake	238:257	High fructose intake has been shown to increase circulating alanine transaminase in humans, which could reflect damage to the liver by fructose but could also be linked to higher level of transamination of amino acids in liver.
32653771	2	64	theme	amino	552:556	arg1	composition					563:573	the amino acid composition	548:573	the amino acid composition in rat plasma and urine	548:597	Therefore, we hypothesized that a diet with high content of fructose would affect the amino acid composition in rat plasma and urine differently from a diet with high sucrose content.
32653771	7	65	theme	cystatin	1582:1589	arg1	C					1591:1591	urine cystatin C	1576:1591	urine cystatin C	1576:1591	Serum concentrations of alanine transaminase, aspartate transaminase, and creatinine, and urine cystatin C and T cell immunoglobulin mucin-1 concentrations were comparable between the groups and within normal ranges.
32653771	4	66	theme	normal	972:977	arg1	content					1005:1011	normal (10 wt%) or high (40 wt%) content	972:1011	normal (10 wt%) or high (40 wt%) content of sucrose or fructose	972:1034	Male Wistar rats were fed diets with normal (10 wt%) or high (40 wt%) content of sucrose or fructose, with normal or high fat content (7 or 22 wt%) and 20 wt% protein (casein).
32653771	5	67	theme	high-fat	1351:1358	arg1	diet					1360:1363	high-sucrose high-fat diet	1338:1363	high-sucrose high-fat diet	1338:1363	Rats fed high-fructose high-fat diet had higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine and higher urine concentrations of arginine and citrulline when compared to rats fed high-sucrose high-fat diet.
32653771	1	68	theme	higher	410:415	arg1	level					417:421	higher level	410:421	higher level of transamination of amino acids in liver	410:463	High fructose intake has been shown to increase circulating alanine transaminase in humans, which could reflect damage to the liver by fructose but could also be linked to higher level of transamination of amino acids in liver.
32653771	3	69	from	effects	801:807	arg1	diets					837:841	diets	837:841	diets with normal or high content of coconut oil	837:884	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	3	69	from	effects	801:807	arg1	urine					928:932	urine	928:932	urine	928:932	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	3	69	from	effects	801:807	arg1	plasma					917:922	plasma	917:922	plasma	917:922	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	3	69	from	effects	801:807	arg1	acids					911:915	individual free amino acids plasma and urine	889:932	individual free amino acids plasma and urine	889:932	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	4	70	theme	wt	1000:1001	arg1	%					1002:1002	40 wt%	997:1002	40 wt%	997:1002	Male Wistar rats were fed diets with normal (10 wt%) or high (40 wt%) content of sucrose or fructose, with normal or high fat content (7 or 22 wt%) and 20 wt% protein (casein).
32653771	3	71	theme	sucrose	673:679	arg1	intake					663:668	high intake	658:668	high intake of sucrose and fructose	658:692	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	0	72	theme	urine	189:193	arg1	concentrations					195:208	higher urine concentrations	182:208	higher urine concentrations of arginine and citrulline	182:235	Substitution of high-dose sucrose with fructose in high-fat diets resulted in higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline.
32653771	7	73	theme	alanine	1510:1516	arg1	transaminase					1518:1529	alanine transaminase	1510:1529	alanine transaminase	1510:1529	Serum concentrations of alanine transaminase, aspartate transaminase, and creatinine, and urine cystatin C and T cell immunoglobulin mucin-1 concentrations were comparable between the groups and within normal ranges.
32653771	2	74	theme	rat	578:580	arg1	plasma					582:587	rat plasma	578:587	rat plasma	578:587	Therefore, we hypothesized that a diet with high content of fructose would affect the amino acid composition in rat plasma and urine differently from a diet with high sucrose content.
32653771	7	75	theme	creatinine	1560:1569	arg1	concentrations					1627:1640	urine cystatin C and T cell immunoglobulin mucin-1 concentrations	1576:1640	urine cystatin C and T cell immunoglobulin mucin-1 concentrations	1576:1640	Serum concentrations of alanine transaminase, aspartate transaminase, and creatinine, and urine cystatin C and T cell immunoglobulin mucin-1 concentrations were comparable between the groups and within normal ranges.
32653771	7	75	theme	creatinine	1560:1569	arg1	concentrations					1492:1505	Serum concentrations	1486:1505	Serum concentrations of alanine transaminase, aspartate transaminase, and creatinine	1486:1569	Serum concentrations of alanine transaminase, aspartate transaminase, and creatinine, and urine cystatin C and T cell immunoglobulin mucin-1 concentrations were comparable between the groups and within normal ranges.
32653771	5	76	theme	cystine	1200:1206	arg1	concentrations					1270:1283	higher urine concentrations	1257:1283	higher urine concentrations of arginine and citrulline	1257:1310	Rats fed high-fructose high-fat diet had higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine and higher urine concentrations of arginine and citrulline when compared to rats fed high-sucrose high-fat diet.
32653771	5	76	theme	cystine	1200:1206	arg1	concentrations					1167:1180	higher plasma concentrations	1153:1180	higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine	1153:1251	Rats fed high-fructose high-fat diet had higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine and higher urine concentrations of arginine and citrulline when compared to rats fed high-sucrose high-fat diet.
32653771	1	77	theme	acids	450:454	arg1	transamination					426:439	transamination	426:439	transamination of amino acids in liver	426:463	High fructose intake has been shown to increase circulating alanine transaminase in humans, which could reflect damage to the liver by fructose but could also be linked to higher level of transamination of amino acids in liver.
32653771	3	78	theme	fructose	685:692	arg1	intake					663:668	high intake	658:668	high intake of sucrose and fructose	658:692	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	8	79	theme	substituting	1716:1727	arg1	sucrose					1739:1745	substituting high-dose sucrose	1716:1745	substituting high-dose sucrose with high-dose fructose in high-fat diets	1716:1787	To conclude, substituting high-dose sucrose with high-dose fructose in high-fat diets affected amino acid compositions in plasma and urine.
32653771	3	80	dep	acids	911:915	arg1	urine					928:932	urine	928:932	urine	928:932	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	3	80	dep	acids	911:915	arg1	plasma					917:922	plasma	917:922	plasma	917:922	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	3	80	dep	acids	911:915	arg1	acids					911:915	individual free amino acids plasma and urine	889:932	individual free amino acids plasma and urine	889:932	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	8	81	theme	amino	1798:1802	arg1	compositions					1809:1820	amino acid compositions	1798:1820	amino acid compositions in plasma and urine	1798:1840	To conclude, substituting high-dose sucrose with high-dose fructose in high-fat diets affected amino acid compositions in plasma and urine.
32653771	0	82	theme	acid	143:146	arg1	concentrations					92:105	higher plasma concentrations	78:105	higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline	78:235	Substitution of high-dose sucrose with fructose in high-fat diets resulted in higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline.
32653771	4	83	dep	high	991:994	arg1	%					1002:1002	40 wt%	997:1002	40 wt%	997:1002	Male Wistar rats were fed diets with normal (10 wt%) or high (40 wt%) content of sucrose or fructose, with normal or high fat content (7 or 22 wt%) and 20 wt% protein (casein).
32653771	6	84	from	content	1386:1392	arg1	diets					1426:1430	the diets	1422:1430	the diets	1422:1430	Substituting normal content of sucrose with fructose in the diets had little impact on amino acids in plasma and urine.
32653771	2	85	theme	fructose	526:533	arg1	content					515:521	high content	510:521	high content of fructose	510:533	Therefore, we hypothesized that a diet with high content of fructose would affect the amino acid composition in rat plasma and urine differently from a diet with high sucrose content.
32653771	8	86	from	compositions	1809:1820	arg1	urine					1836:1840	urine	1836:1840	urine	1836:1840	To conclude, substituting high-dose sucrose with high-dose fructose in high-fat diets affected amino acid compositions in plasma and urine.
32653771	8	86	from	compositions	1809:1820	arg1	plasma					1825:1830	plasma	1825:1830	plasma	1825:1830	To conclude, substituting high-dose sucrose with high-dose fructose in high-fat diets affected amino acid compositions in plasma and urine.
32653771	4	87	theme	normal	1042:1047	arg1	content					1061:1067	normal or high fat content	1042:1067	content	1061:1067	Male Wistar rats were fed diets with normal (10 wt%) or high (40 wt%) content of sucrose or fructose, with normal or high fat content (7 or 22 wt%) and 20 wt% protein (casein).
32653771	4	87	theme	normal	1042:1047	arg1	%					1080:1080	7 or 22 wt%	1070:1080	7 or 22 wt%	1070:1080	Male Wistar rats were fed diets with normal (10 wt%) or high (40 wt%) content of sucrose or fructose, with normal or high fat content (7 or 22 wt%) and 20 wt% protein (casein).
32653771	5	88	theme	glutamic	1209:1216	arg1	acid					1218:1221	glutamic acid	1209:1221	glutamic acid	1209:1221	Rats fed high-fructose high-fat diet had higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine and higher urine concentrations of arginine and citrulline when compared to rats fed high-sucrose high-fat diet.
32653771	8	89	theme	high-dose	1752:1760	arg1	fructose					1762:1769	high-dose fructose	1752:1769	high-dose fructose	1752:1769	To conclude, substituting high-dose sucrose with high-dose fructose in high-fat diets affected amino acid compositions in plasma and urine.
32653771	6	90	theme	little	1436:1441	arg1	impact					1443:1448	little impact	1436:1448	little impact	1436:1448	Substituting normal content of sucrose with fructose in the diets had little impact on amino acids in plasma and urine.
32653771	7	91	theme	transaminase	1542:1553	arg1	concentrations					1627:1640	urine cystatin C and T cell immunoglobulin mucin-1 concentrations	1576:1640	urine cystatin C and T cell immunoglobulin mucin-1 concentrations	1576:1640	Serum concentrations of alanine transaminase, aspartate transaminase, and creatinine, and urine cystatin C and T cell immunoglobulin mucin-1 concentrations were comparable between the groups and within normal ranges.
32653771	7	91	theme	transaminase	1542:1553	arg1	concentrations					1492:1505	Serum concentrations	1486:1505	Serum concentrations of alanine transaminase, aspartate transaminase, and creatinine	1486:1569	Serum concentrations of alanine transaminase, aspartate transaminase, and creatinine, and urine cystatin C and T cell immunoglobulin mucin-1 concentrations were comparable between the groups and within normal ranges.
32653771	5	92	theme	acid	1194:1197	arg1	concentrations					1270:1283	higher urine concentrations	1257:1283	higher urine concentrations of arginine and citrulline	1257:1310	Rats fed high-fructose high-fat diet had higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine and higher urine concentrations of arginine and citrulline when compared to rats fed high-sucrose high-fat diet.
32653771	5	92	theme	acid	1194:1197	arg1	concentrations					1167:1180	higher plasma concentrations	1153:1180	higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine	1153:1251	Rats fed high-fructose high-fat diet had higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine and higher urine concentrations of arginine and citrulline when compared to rats fed high-sucrose high-fat diet.
32653771	6	93	theme	amino	1453:1457	arg1	acids					1459:1463	amino acids	1453:1463	amino acids in plasma and urine	1453:1483	Substituting normal content of sucrose with fructose in the diets had little impact on amino acids in plasma and urine.
32653771	6	94	with	content	1386:1392	arg1	fructose					1410:1417	fructose	1410:1417	fructose	1410:1417	Substituting normal content of sucrose with fructose in the diets had little impact on amino acids in plasma and urine.
32653771	3	95	theme	saturated	735:743	arg1	fat					745:747	saturated fat	735:747	saturated fat	735:747	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	0	96	theme	higher	78:83	arg1	concentrations					92:105	higher plasma concentrations	78:105	higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline	78:235	Substitution of high-dose sucrose with fructose in high-fat diets resulted in higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline.
32653771	3	97	theme	high	812:815	arg1	fructose/sucrose					817:832	high fructose/sucrose	812:832	high fructose/sucrose in diets with normal or high content of coconut oil	812:884	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	5	98	theme	arginine	1288:1295	arg1	concentrations					1270:1283	higher urine concentrations	1257:1283	higher urine concentrations of arginine and citrulline	1257:1310	Rats fed high-fructose high-fat diet had higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine and higher urine concentrations of arginine and citrulline when compared to rats fed high-sucrose high-fat diet.
32653771	5	98	theme	arginine	1288:1295	arg1	concentrations					1167:1180	higher plasma concentrations	1153:1180	higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine	1153:1251	Rats fed high-fructose high-fat diet had higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine and higher urine concentrations of arginine and citrulline when compared to rats fed high-sucrose high-fat diet.
32653771	8	99	with	sucrose	1739:1745	arg1	fructose					1762:1769	high-dose fructose	1752:1769	high-dose fructose	1752:1769	To conclude, substituting high-dose sucrose with high-dose fructose in high-fat diets affected amino acid compositions in plasma and urine.
32653771	2	100	with	diet	618:621	arg1	content					641:647	high sucrose content	628:647	high sucrose content	628:647	Therefore, we hypothesized that a diet with high content of fructose would affect the amino acid composition in rat plasma and urine differently from a diet with high sucrose content.
32653771	0	101	theme	arginine	213:220	arg1	acid					143:146	glutamic acid	134:146	glutamic acid	134:146	Substitution of high-dose sucrose with fructose in high-fat diets resulted in higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline.
32653771	0	101	theme	arginine	213:220	arg1	ornithine					149:157	ornithine	149:157	ornithine	149:157	Substitution of high-dose sucrose with fructose in high-fat diets resulted in higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline.
32653771	0	101	theme	arginine	213:220	arg1	acid					119:122	aspartic acid	110:122	aspartic acid	110:122	Substitution of high-dose sucrose with fructose in high-fat diets resulted in higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline.
32653771	0	101	theme	arginine	213:220	arg1	concentrations					195:208	higher urine concentrations	182:208	higher urine concentrations of arginine and citrulline	182:235	Substitution of high-dose sucrose with fructose in high-fat diets resulted in higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline.
32653771	0	101	theme	arginine	213:220	arg1	cystine					125:131	cystine	125:131	cystine	125:131	Substitution of high-dose sucrose with fructose in high-fat diets resulted in higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline.
32653771	0	101	theme	arginine	213:220	arg1	phenylalanine					163:175	phenylalanine	163:175	phenylalanine	163:175	Substitution of high-dose sucrose with fructose in high-fat diets resulted in higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline.
32653771	7	102	theme	cell	1599:1602	arg1	mucin-1					1619:1625	T cell immunoglobulin mucin-1	1597:1625	T cell immunoglobulin mucin-1	1597:1625	Serum concentrations of alanine transaminase, aspartate transaminase, and creatinine, and urine cystatin C and T cell immunoglobulin mucin-1 concentrations were comparable between the groups and within normal ranges.
32653771	3	103	from	intake	725:730	arg1	diet					770:773	the Western-style diet	752:773	the Western-style diet	752:773	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	3	104	theme	Western-style	756:768	arg1	diet					770:773	the Western-style diet	752:773	the Western-style diet	752:773	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	0	105	theme	aspartic	110:117	arg1	acid					119:122	aspartic acid	110:122	aspartic acid	110:122	Substitution of high-dose sucrose with fructose in high-fat diets resulted in higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline.
32653771	0	106	theme	citrulline	226:235	arg1	acid					143:146	glutamic acid	134:146	glutamic acid	134:146	Substitution of high-dose sucrose with fructose in high-fat diets resulted in higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline.
32653771	0	106	theme	citrulline	226:235	arg1	ornithine					149:157	ornithine	149:157	ornithine	149:157	Substitution of high-dose sucrose with fructose in high-fat diets resulted in higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline.
32653771	0	106	theme	citrulline	226:235	arg1	acid					119:122	aspartic acid	110:122	aspartic acid	110:122	Substitution of high-dose sucrose with fructose in high-fat diets resulted in higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline.
32653771	0	106	theme	citrulline	226:235	arg1	concentrations					195:208	higher urine concentrations	182:208	higher urine concentrations of arginine and citrulline	182:235	Substitution of high-dose sucrose with fructose in high-fat diets resulted in higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline.
32653771	0	106	theme	citrulline	226:235	arg1	cystine					125:131	cystine	125:131	cystine	125:131	Substitution of high-dose sucrose with fructose in high-fat diets resulted in higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline.
32653771	0	106	theme	citrulline	226:235	arg1	phenylalanine					163:175	phenylalanine	163:175	phenylalanine	163:175	Substitution of high-dose sucrose with fructose in high-fat diets resulted in higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline.
32653771	5	107	theme	phenylalanine	1239:1251	arg1	concentrations					1270:1283	higher urine concentrations	1257:1283	higher urine concentrations of arginine and citrulline	1257:1310	Rats fed high-fructose high-fat diet had higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine and higher urine concentrations of arginine and citrulline when compared to rats fed high-sucrose high-fat diet.
32653771	5	107	theme	phenylalanine	1239:1251	arg1	concentrations					1167:1180	higher plasma concentrations	1153:1180	higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine	1153:1251	Rats fed high-fructose high-fat diet had higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine and higher urine concentrations of arginine and citrulline when compared to rats fed high-sucrose high-fat diet.
32653771	4	108	theme	high	1052:1055	arg1	content					1061:1067	normal or high fat content	1042:1067	content	1061:1067	Male Wistar rats were fed diets with normal (10 wt%) or high (40 wt%) content of sucrose or fructose, with normal or high fat content (7 or 22 wt%) and 20 wt% protein (casein).
32653771	4	108	theme	high	1052:1055	arg1	%					1080:1080	7 or 22 wt%	1070:1080	7 or 22 wt%	1070:1080	Male Wistar rats were fed diets with normal (10 wt%) or high (40 wt%) content of sucrose or fructose, with normal or high fat content (7 or 22 wt%) and 20 wt% protein (casein).
32653771	5	109	theme	high-fructose	1121:1133	arg1	diet					1144:1147	high-fructose high-fat diet	1121:1147	high-fructose high-fat diet	1121:1147	Rats fed high-fructose high-fat diet had higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine and higher urine concentrations of arginine and citrulline when compared to rats fed high-sucrose high-fat diet.
32653771	4	110	with	diets	961:965	arg1	content					1005:1011	normal (10 wt%) or high (40 wt%) content	972:1011	normal (10 wt%) or high (40 wt%) content of sucrose or fructose	972:1034	Male Wistar rats were fed diets with normal (10 wt%) or high (40 wt%) content of sucrose or fructose, with normal or high fat content (7 or 22 wt%) and 20 wt% protein (casein).
32653771	4	111	theme	Male	935:938	arg1	rats					947:950	Male Wistar rats	935:950	Male Wistar rats	935:950	Male Wistar rats were fed diets with normal (10 wt%) or high (40 wt%) content of sucrose or fructose, with normal or high fat content (7 or 22 wt%) and 20 wt% protein (casein).
32653771	5	112	theme	higher	1257:1262	arg1	concentrations					1270:1283	higher urine concentrations	1257:1283	higher urine concentrations of arginine and citrulline	1257:1310	Rats fed high-fructose high-fat diet had higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine and higher urine concentrations of arginine and citrulline when compared to rats fed high-sucrose high-fat diet.
32653771	0	113	theme	sucrose	26:32	arg1	Substitution					0:11	Substitution	0:11	Substitution of high-dose sucrose with fructose in high-fat diets	0:64	Substitution of high-dose sucrose with fructose in high-fat diets resulted in higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline.
32653771	4	114	theme	wt	1090:1091	arg1	casein					1103:1108	20 wt% protein (casein)	1087:1109	20 wt% protein (casein)	1087:1109	Male Wistar rats were fed diets with normal (10 wt%) or high (40 wt%) content of sucrose or fructose, with normal or high fat content (7 or 22 wt%) and 20 wt% protein (casein).
32653771	5	115	theme	high-sucrose	1338:1349	arg1	diet					1360:1363	high-sucrose high-fat diet	1338:1363	high-sucrose high-fat diet	1338:1363	Rats fed high-fructose high-fat diet had higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine and higher urine concentrations of arginine and citrulline when compared to rats fed high-sucrose high-fat diet.
32653771	4	116	theme	protein	1094:1100	arg1	casein					1103:1108	20 wt% protein (casein)	1087:1109	20 wt% protein (casein)	1087:1109	Male Wistar rats were fed diets with normal (10 wt%) or high (40 wt%) content of sucrose or fructose, with normal or high fat content (7 or 22 wt%) and 20 wt% protein (casein).
32653771	0	117	theme	high-fat	51:58	arg1	diets					60:64	high-fat diets	51:64	high-fat diets	51:64	Substitution of high-dose sucrose with fructose in high-fat diets resulted in higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine and phenylalanine, and higher urine concentrations of arginine and citrulline.
32653771	1	118	theme	alanine	298:304	arg1	transaminase					306:317	circulating alanine transaminase	286:317	circulating alanine transaminase	286:317	High fructose intake has been shown to increase circulating alanine transaminase in humans, which could reflect damage to the liver by fructose but could also be linked to higher level of transamination of amino acids in liver.
32653771	6	119	from	acids	1459:1463	arg1	urine					1479:1483	urine	1479:1483	urine	1479:1483	Substituting normal content of sucrose with fructose in the diets had little impact on amino acids in plasma and urine.
32653771	6	119	from	acids	1459:1463	arg1	plasma					1468:1473	plasma	1468:1473	plasma	1468:1473	Substituting normal content of sucrose with fructose in the diets had little impact on amino acids in plasma and urine.
32653771	5	120	theme	citrulline	1301:1310	arg1	concentrations					1270:1283	higher urine concentrations	1257:1283	higher urine concentrations of arginine and citrulline	1257:1310	Rats fed high-fructose high-fat diet had higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine and higher urine concentrations of arginine and citrulline when compared to rats fed high-sucrose high-fat diet.
32653771	5	120	theme	citrulline	1301:1310	arg1	concentrations					1167:1180	higher plasma concentrations	1153:1180	higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine	1153:1251	Rats fed high-fructose high-fat diet had higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine and higher urine concentrations of arginine and citrulline when compared to rats fed high-sucrose high-fat diet.
32653771	5	121	theme	aspartic	1185:1192	arg1	acid					1194:1197	aspartic acid	1185:1197	aspartic acid	1185:1197	Rats fed high-fructose high-fat diet had higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine and higher urine concentrations of arginine and citrulline when compared to rats fed high-sucrose high-fat diet.
32653771	2	122	with	diet	500:503	arg1	content					515:521	high content	510:521	high content of fructose	510:533	Therefore, we hypothesized that a diet with high content of fructose would affect the amino acid composition in rat plasma and urine differently from a diet with high sucrose content.
32653771	2	123	theme	high	628:631	arg1	content					641:647	high sucrose content	628:647	high sucrose content	628:647	Therefore, we hypothesized that a diet with high content of fructose would affect the amino acid composition in rat plasma and urine differently from a diet with high sucrose content.
32653771	3	124	theme	coconut	874:880	arg1	oil					882:884	coconut oil	874:884	coconut oil	874:884	Because high intake of sucrose and fructose is often accompanied with high intake of saturated fat in the Western-style diet, we wanted to compare the effects of high fructose/sucrose in diets with normal or high content of coconut oil on individual free amino acids plasma and urine.
32653771	1	125	from	transamination	426:439	arg1	liver					459:463	liver	459:463	liver	459:463	High fructose intake has been shown to increase circulating alanine transaminase in humans, which could reflect damage to the liver by fructose but could also be linked to higher level of transamination of amino acids in liver.
32653771	7	126	theme	immunoglobulin	1604:1617	arg1	mucin-1					1619:1625	T cell immunoglobulin mucin-1	1597:1625	T cell immunoglobulin mucin-1	1597:1625	Serum concentrations of alanine transaminase, aspartate transaminase, and creatinine, and urine cystatin C and T cell immunoglobulin mucin-1 concentrations were comparable between the groups and within normal ranges.
32653771	4	127	theme	wt	983:984	arg1	%					985:985	10 wt%	980:985	10 wt%	980:985	Male Wistar rats were fed diets with normal (10 wt%) or high (40 wt%) content of sucrose or fructose, with normal or high fat content (7 or 22 wt%) and 20 wt% protein (casein).
32653771	5	128	theme	higher	1153:1158	arg1	concentrations					1167:1180	higher plasma concentrations	1153:1180	higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine	1153:1251	Rats fed high-fructose high-fat diet had higher plasma concentrations of aspartic acid, cystine, glutamic acid, ornithine, and phenylalanine and higher urine concentrations of arginine and citrulline when compared to rats fed high-sucrose high-fat diet.
32955050	6	0	theme	TLR4	918:921	arg1	pathway					934:940	lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4) signaling pathway	871:940	lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4) signaling pathway in the WAT	871:951	Western blotting was employed to evaluate the relative protein expressions involved in lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4) signaling pathway in the WAT.
32955050	7	1	theme	alpha	1108:1112	arg1	levels					1068:1073	serum levels	1062:1073	serum levels of LPS and tumor necrosis factor alpha, which is associated with an improvement in intestinal epithelial integrity and probiotic composition as well as a reduction in Gram-negative Proteobacteria and Deferribacteres	1062:1289	Treatment of obese mice with Sal B improves insulin sensitivity, attenuates body weight gain and alleviates serum levels of LPS and tumor necrosis factor alpha, which is associated with an improvement in intestinal epithelial integrity and probiotic composition as well as a reduction in Gram-negative Proteobacteria and Deferribacteres.
32955050	3	2	theme	histopathological	513:529	arg1	alterations					531:541	histopathological alterations	513:541	histopathological alterations in the white adipose tissue (WAT) and/or jejunums	513:591	Hematoxylin/eosin staining, transmission electron microscopy, and immunohistochemical staining were used to evaluate histopathological alterations in the white adipose tissue (WAT) and/or jejunums.
32955050	9	3	theme	gut	1621:1623	arg1	abundances					1636:1645	gut microbiota abundances	1621:1645	gut microbiota abundances	1621:1645	In summary, Sal B may attenuate body weight gain and insulin resistance through the regulation of gut microbiota abundances and LPS/TLR4 signaling pathway in obese mice, suggesting Sal B could be a promising drug candidate for protection against obesity.
32955050	4	4	theme	expression	598:607	arg1	levels					609:614	The expression levels	594:614	The expression levels of genes related to fat and cholesterol synthesis in the WAT	594:675	The expression levels of genes related to fat and cholesterol synthesis in the WAT were determined by qPCR.
32955050	7	5	theme	necrosis	1092:1099	arg1	alpha					1108:1112	tumor necrosis factor alpha	1086:1112	tumor necrosis factor alpha	1086:1112	Treatment of obese mice with Sal B improves insulin sensitivity, attenuates body weight gain and alleviates serum levels of LPS and tumor necrosis factor alpha, which is associated with an improvement in intestinal epithelial integrity and probiotic composition as well as a reduction in Gram-negative Proteobacteria and Deferribacteres.
32955050	3	6	from	alterations	531:541	arg1	jejunums					584:591	jejunums	584:591	jejunums	584:591	Hematoxylin/eosin staining, transmission electron microscopy, and immunohistochemical staining were used to evaluate histopathological alterations in the white adipose tissue (WAT) and/or jejunums.
32955050	3	6	from	alterations	531:541	arg1	tissue					564:569	the white adipose tissue	546:569	the white adipose tissue (WAT)	546:575	Hematoxylin/eosin staining, transmission electron microscopy, and immunohistochemical staining were used to evaluate histopathological alterations in the white adipose tissue (WAT) and/or jejunums.
32955050	6	7	theme	/toll-like	895:904	arg1	pathway					934:940	lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4) signaling pathway	871:940	lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4) signaling pathway in the WAT	871:951	Western blotting was employed to evaluate the relative protein expressions involved in lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4) signaling pathway in the WAT.
32955050	9	8	theme	abundances	1636:1645	arg1	regulation					1607:1616	the regulation	1603:1616	the regulation of gut microbiota abundances and LPS/TLR4 signaling pathway in obese mice, suggesting Sal B could be a promising drug candidate for protection against obesity	1603:1775	In summary, Sal B may attenuate body weight gain and insulin resistance through the regulation of gut microbiota abundances and LPS/TLR4 signaling pathway in obese mice, suggesting Sal B could be a promising drug candidate for protection against obesity.
32955050	3	9	theme	white	550:554	arg1	tissue					564:569	the white adipose tissue	546:569	the white adipose tissue (WAT)	546:575	Hematoxylin/eosin staining, transmission electron microscopy, and immunohistochemical staining were used to evaluate histopathological alterations in the white adipose tissue (WAT) and/or jejunums.
32955050	1	10	theme	Sal	162:164	arg1	B					166:166	Sal B	162:166	Sal B	162:166	Salvianolic acid B (Sal B) exhibits anti-obesity activity, yet the underlying mechanism linking this effect to metabolic endotoxemia remains unexplored.
32955050	1	10	theme	Sal	162:164	arg1	B					159:159	Salvianolic acid B	142:159	Salvianolic acid B (Sal B)	142:167	Salvianolic acid B (Sal B) exhibits anti-obesity activity, yet the underlying mechanism linking this effect to metabolic endotoxemia remains unexplored.
32955050	9	11	theme	LPS/TLR4	1651:1658	arg1	pathway					1670:1676	LPS/TLR4 signaling pathway	1651:1676	LPS/TLR4 signaling pathway	1651:1676	In summary, Sal B may attenuate body weight gain and insulin resistance through the regulation of gut microbiota abundances and LPS/TLR4 signaling pathway in obese mice, suggesting Sal B could be a promising drug candidate for protection against obesity.
32955050	9	12	theme	pathway	1670:1676	arg1	regulation					1607:1616	the regulation	1603:1616	the regulation of gut microbiota abundances and LPS/TLR4 signaling pathway in obese mice, suggesting Sal B could be a promising drug candidate for protection against obesity	1603:1775	In summary, Sal B may attenuate body weight gain and insulin resistance through the regulation of gut microbiota abundances and LPS/TLR4 signaling pathway in obese mice, suggesting Sal B could be a promising drug candidate for protection against obesity.
32955050	7	13	theme	serum	1062:1066	arg1	levels					1068:1073	serum levels	1062:1073	serum levels of LPS and tumor necrosis factor alpha, which is associated with an improvement in intestinal epithelial integrity and probiotic composition as well as a reduction in Gram-negative Proteobacteria and Deferribacteres	1062:1289	Treatment of obese mice with Sal B improves insulin sensitivity, attenuates body weight gain and alleviates serum levels of LPS and tumor necrosis factor alpha, which is associated with an improvement in intestinal epithelial integrity and probiotic composition as well as a reduction in Gram-negative Proteobacteria and Deferribacteres.
32955050	4	14	from	fat	636:638	arg1	the					669:671	the	669:671	the	669:671	The expression levels of genes related to fat and cholesterol synthesis in the WAT were determined by qPCR.
32955050	1	15	theme	anti-obesity	178:189	arg1	activity					191:198	anti-obesity activity	178:198	anti-obesity activity	178:198	Salvianolic acid B (Sal B) exhibits anti-obesity activity, yet the underlying mechanism linking this effect to metabolic endotoxemia remains unexplored.
32955050	9	16	theme	obese	1681:1685	arg1	mice					1687:1690	obese mice	1681:1690	obese mice	1681:1690	In summary, Sal B may attenuate body weight gain and insulin resistance through the regulation of gut microbiota abundances and LPS/TLR4 signaling pathway in obese mice, suggesting Sal B could be a promising drug candidate for protection against obesity.
32955050	8	17	theme	N-terminal	1430:1439	arg1	kinase					1441:1446	Jun N-terminal kinase	1426:1446	Jun N-terminal kinase	1426:1446	In addition, Sal B downregulates the expressions of TLR4 and myeloid differential factor-88, as well as the phosphorylation levels of Jun N-terminal kinase, nuclear factor-kappa B p65, and an insulin receptor substrate in the WAT.
32955050	4	18	theme	cholesterol	644:654	arg1	synthesis					656:664	cholesterol synthesis	644:664	cholesterol synthesis	644:664	The expression levels of genes related to fat and cholesterol synthesis in the WAT were determined by qPCR.
32955050	0	19	theme	obese	130:134	arg1	mice					136:139	high-fat diet-induced obese mice	108:139	high-fat diet-induced obese mice	108:139	Salvianolic acid B prevents body weight gain and regulates gut microbiota and LPS/TLR4 signaling pathway in high-fat diet-induced obese mice.
32955050	7	20	theme	insulin	998:1004	arg1	sensitivity					1006:1016	insulin sensitivity	998:1016	insulin sensitivity	998:1016	Treatment of obese mice with Sal B improves insulin sensitivity, attenuates body weight gain and alleviates serum levels of LPS and tumor necrosis factor alpha, which is associated with an improvement in intestinal epithelial integrity and probiotic composition as well as a reduction in Gram-negative Proteobacteria and Deferribacteres.
32955050	7	21	theme	weight	1035:1040	arg1	gain					1042:1045	body weight gain	1030:1045	body weight gain	1030:1045	Treatment of obese mice with Sal B improves insulin sensitivity, attenuates body weight gain and alleviates serum levels of LPS and tumor necrosis factor alpha, which is associated with an improvement in intestinal epithelial integrity and probiotic composition as well as a reduction in Gram-negative Proteobacteria and Deferribacteres.
32955050	8	22	theme	factor-kappa	1457:1468	arg1	B					1470:1470	nuclear factor-kappa B	1449:1470	nuclear factor-kappa B p65	1449:1474	In addition, Sal B downregulates the expressions of TLR4 and myeloid differential factor-88, as well as the phosphorylation levels of Jun N-terminal kinase, nuclear factor-kappa B p65, and an insulin receptor substrate in the WAT.
32955050	6	23	theme	signaling	924:932	arg1	pathway					934:940	lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4) signaling pathway	871:940	lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4) signaling pathway in the WAT	871:951	Western blotting was employed to evaluate the relative protein expressions involved in lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4) signaling pathway in the WAT.
32955050	9	24	theme	Sal	1704:1706	arg1	candidate					1736:1744	a promising drug candidate	1719:1744	a promising drug candidate for protection against obesity	1719:1775	In summary, Sal B may attenuate body weight gain and insulin resistance through the regulation of gut microbiota abundances and LPS/TLR4 signaling pathway in obese mice, suggesting Sal B could be a promising drug candidate for protection against obesity.
32955050	9	24	theme	Sal	1704:1706	arg1	B					1708:1708	Sal B	1704:1708	Sal B	1704:1708	In summary, Sal B may attenuate body weight gain and insulin resistance through the regulation of gut microbiota abundances and LPS/TLR4 signaling pathway in obese mice, suggesting Sal B could be a promising drug candidate for protection against obesity.
32955050	0	25	from	pathway	97:103	arg1	mice					136:139	high-fat diet-induced obese mice	108:139	high-fat diet-induced obese mice	108:139	Salvianolic acid B prevents body weight gain and regulates gut microbiota and LPS/TLR4 signaling pathway in high-fat diet-induced obese mice.
32955050	8	26	theme	p65	1472:1474	arg1	levels					1416:1421	the phosphorylation levels	1396:1421	the phosphorylation levels of Jun N-terminal kinase, nuclear factor-kappa B p65, and an insulin receptor substrate	1396:1509	In addition, Sal B downregulates the expressions of TLR4 and myeloid differential factor-88, as well as the phosphorylation levels of Jun N-terminal kinase, nuclear factor-kappa B p65, and an insulin receptor substrate in the WAT.
32955050	8	26	theme	p65	1472:1474	arg1	expressions					1329:1339	the expressions	1325:1339	the expressions of TLR4 and myeloid differential factor-88	1325:1382	In addition, Sal B downregulates the expressions of TLR4 and myeloid differential factor-88, as well as the phosphorylation levels of Jun N-terminal kinase, nuclear factor-kappa B p65, and an insulin receptor substrate in the WAT.
32955050	9	27	dep	mice	1687:1690	arg1	suggesting					1693:1702	suggesting	1693:1702	suggesting Sal B could be a promising drug candidate for protection against obesity	1693:1775	In summary, Sal B may attenuate body weight gain and insulin resistance through the regulation of gut microbiota abundances and LPS/TLR4 signaling pathway in obese mice, suggesting Sal B could be a promising drug candidate for protection against obesity.
32955050	7	28	theme	obese	967:971	arg1	mice					973:976	obese mice	967:976	obese mice	967:976	Treatment of obese mice with Sal B improves insulin sensitivity, attenuates body weight gain and alleviates serum levels of LPS and tumor necrosis factor alpha, which is associated with an improvement in intestinal epithelial integrity and probiotic composition as well as a reduction in Gram-negative Proteobacteria and Deferribacteres.
32955050	1	29	theme	Salvianolic	142:152	arg1	B					166:166	Sal B	162:166	Sal B	162:166	Salvianolic acid B (Sal B) exhibits anti-obesity activity, yet the underlying mechanism linking this effect to metabolic endotoxemia remains unexplored.
32955050	1	29	theme	Salvianolic	142:152	arg1	B					159:159	Salvianolic acid B	142:159	Salvianolic acid B (Sal B)	142:167	Salvianolic acid B (Sal B) exhibits anti-obesity activity, yet the underlying mechanism linking this effect to metabolic endotoxemia remains unexplored.
32955050	5	30	theme	gene	763:766	arg1	pyrosequencing					768:781	rRNA gene pyrosequencing	758:781	rRNA gene pyrosequencing	758:781	The composition of fecal microbiota was profiled by 16S rRNA gene pyrosequencing.
32955050	9	31	from	mice	1687:1690	arg1	regulation					1607:1616	the regulation	1603:1616	the regulation of gut microbiota abundances and LPS/TLR4 signaling pathway in obese mice, suggesting Sal B could be a promising drug candidate for protection against obesity	1603:1775	In summary, Sal B may attenuate body weight gain and insulin resistance through the regulation of gut microbiota abundances and LPS/TLR4 signaling pathway in obese mice, suggesting Sal B could be a promising drug candidate for protection against obesity.
32955050	9	32	theme	drug	1731:1734	arg1	candidate					1736:1744	a promising drug candidate	1719:1744	a promising drug candidate for protection against obesity	1719:1775	In summary, Sal B may attenuate body weight gain and insulin resistance through the regulation of gut microbiota abundances and LPS/TLR4 signaling pathway in obese mice, suggesting Sal B could be a promising drug candidate for protection against obesity.
32955050	9	32	theme	drug	1731:1734	arg1	B					1708:1708	Sal B	1704:1708	Sal B	1704:1708	In summary, Sal B may attenuate body weight gain and insulin resistance through the regulation of gut microbiota abundances and LPS/TLR4 signaling pathway in obese mice, suggesting Sal B could be a promising drug candidate for protection against obesity.
32955050	8	33	theme	insulin	1484:1490	arg1	substrate					1501:1509	an insulin receptor substrate	1481:1509	an insulin receptor substrate	1481:1509	In addition, Sal B downregulates the expressions of TLR4 and myeloid differential factor-88, as well as the phosphorylation levels of Jun N-terminal kinase, nuclear factor-kappa B p65, and an insulin receptor substrate in the WAT.
32955050	0	34	theme	LPS/TLR4	78:85	arg1	pathway					97:103	LPS/TLR4 signaling pathway	78:103	LPS/TLR4 signaling pathway	78:103	Salvianolic acid B prevents body weight gain and regulates gut microbiota and LPS/TLR4 signaling pathway in high-fat diet-induced obese mice.
32955050	8	35	theme	substrate	1501:1509	arg1	levels					1416:1421	the phosphorylation levels	1396:1421	the phosphorylation levels of Jun N-terminal kinase, nuclear factor-kappa B p65, and an insulin receptor substrate	1396:1509	In addition, Sal B downregulates the expressions of TLR4 and myeloid differential factor-88, as well as the phosphorylation levels of Jun N-terminal kinase, nuclear factor-kappa B p65, and an insulin receptor substrate in the WAT.
32955050	8	35	theme	substrate	1501:1509	arg1	expressions					1329:1339	the expressions	1325:1339	the expressions of TLR4 and myeloid differential factor-88	1325:1382	In addition, Sal B downregulates the expressions of TLR4 and myeloid differential factor-88, as well as the phosphorylation levels of Jun N-terminal kinase, nuclear factor-kappa B p65, and an insulin receptor substrate in the WAT.
32955050	3	36	theme	transmission	424:435	arg1	microscopy					446:455	transmission electron microscopy	424:455	transmission electron microscopy	424:455	Hematoxylin/eosin staining, transmission electron microscopy, and immunohistochemical staining were used to evaluate histopathological alterations in the white adipose tissue (WAT) and/or jejunums.
32955050	7	37	theme	epithelial	1169:1178	arg1	integrity					1180:1188	intestinal epithelial integrity	1158:1188	intestinal epithelial integrity	1158:1188	Treatment of obese mice with Sal B improves insulin sensitivity, attenuates body weight gain and alleviates serum levels of LPS and tumor necrosis factor alpha, which is associated with an improvement in intestinal epithelial integrity and probiotic composition as well as a reduction in Gram-negative Proteobacteria and Deferribacteres.
32955050	8	38	theme	differential	1361:1372	arg1	factor-88					1374:1382	myeloid differential factor-88	1353:1382	myeloid differential factor-88	1353:1382	In addition, Sal B downregulates the expressions of TLR4 and myeloid differential factor-88, as well as the phosphorylation levels of Jun N-terminal kinase, nuclear factor-kappa B p65, and an insulin receptor substrate in the WAT.
32955050	2	39	theme	Sal	376:378	arg1	B					380:380	Sal B	376:380	Sal B	376:380	For this purpose, high-fat diet-induced obese mice were orally administered with Sal B for 10 weeks.
32955050	0	40	theme	Salvianolic	0:10	arg1	B					17:17	Salvianolic acid B	0:17	Salvianolic acid B	0:17	Salvianolic acid B prevents body weight gain and regulates gut microbiota and LPS/TLR4 signaling pathway in high-fat diet-induced obese mice.
32955050	1	41	theme	metabolic	253:261	arg1	endotoxemia					263:273	metabolic endotoxemia	253:273	metabolic endotoxemia	253:273	Salvianolic acid B (Sal B) exhibits anti-obesity activity, yet the underlying mechanism linking this effect to metabolic endotoxemia remains unexplored.
32955050	0	42	theme	body	28:31	arg1	gain					40:43	body weight gain	28:43	body weight gain	28:43	Salvianolic acid B prevents body weight gain and regulates gut microbiota and LPS/TLR4 signaling pathway in high-fat diet-induced obese mice.
32955050	9	43	theme	weight	1560:1565	arg1	gain					1567:1570	body weight gain	1555:1570	body weight gain	1555:1570	In summary, Sal B may attenuate body weight gain and insulin resistance through the regulation of gut microbiota abundances and LPS/TLR4 signaling pathway in obese mice, suggesting Sal B could be a promising drug candidate for protection against obesity.
32955050	6	44	from	pathway	934:940	arg1	the					945:947	the	945:947	the	945:947	Western blotting was employed to evaluate the relative protein expressions involved in lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4) signaling pathway in the WAT.
32955050	2	45	theme	obese	335:339	arg1	mice					341:344	high-fat diet-induced obese mice	313:344	high-fat diet-induced obese mice	313:344	For this purpose, high-fat diet-induced obese mice were orally administered with Sal B for 10 weeks.
32955050	2	46	theme	high-fat	313:320	arg1	mice					341:344	high-fat diet-induced obese mice	313:344	high-fat diet-induced obese mice	313:344	For this purpose, high-fat diet-induced obese mice were orally administered with Sal B for 10 weeks.
32955050	6	47	theme	relative	830:837	arg1	expressions					847:857	the relative protein expressions	826:857	the relative protein expressions involved in lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4) signaling pathway in the WAT	826:951	Western blotting was employed to evaluate the relative protein expressions involved in lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4) signaling pathway in the WAT.
32955050	8	48	theme	Jun	1426:1428	arg1	kinase					1441:1446	Jun N-terminal kinase	1426:1446	Jun N-terminal kinase	1426:1446	In addition, Sal B downregulates the expressions of TLR4 and myeloid differential factor-88, as well as the phosphorylation levels of Jun N-terminal kinase, nuclear factor-kappa B p65, and an insulin receptor substrate in the WAT.
32955050	1	49	theme	underlying	209:218	arg1	mechanism					220:228	the underlying mechanism	205:228	the underlying mechanism linking this effect to metabolic endotoxemia	205:273	Salvianolic acid B (Sal B) exhibits anti-obesity activity, yet the underlying mechanism linking this effect to metabolic endotoxemia remains unexplored.
32955050	4	50	theme	genes	619:623	arg1	levels					609:614	The expression levels	594:614	The expression levels of genes related to fat and cholesterol synthesis in the WAT	594:675	The expression levels of genes related to fat and cholesterol synthesis in the WAT were determined by qPCR.
32955050	7	51	theme	probiotic	1194:1202	arg1	composition					1204:1214	probiotic composition	1194:1214	probiotic composition	1194:1214	Treatment of obese mice with Sal B improves insulin sensitivity, attenuates body weight gain and alleviates serum levels of LPS and tumor necrosis factor alpha, which is associated with an improvement in intestinal epithelial integrity and probiotic composition as well as a reduction in Gram-negative Proteobacteria and Deferribacteres.
32955050	7	52	theme	factor	1101:1106	arg1	alpha					1108:1112	tumor necrosis factor alpha	1086:1112	tumor necrosis factor alpha	1086:1112	Treatment of obese mice with Sal B improves insulin sensitivity, attenuates body weight gain and alleviates serum levels of LPS and tumor necrosis factor alpha, which is associated with an improvement in intestinal epithelial integrity and probiotic composition as well as a reduction in Gram-negative Proteobacteria and Deferribacteres.
32955050	7	53	theme	tumor	1086:1090	arg1	alpha					1108:1112	tumor necrosis factor alpha	1086:1112	tumor necrosis factor alpha	1086:1112	Treatment of obese mice with Sal B improves insulin sensitivity, attenuates body weight gain and alleviates serum levels of LPS and tumor necrosis factor alpha, which is associated with an improvement in intestinal epithelial integrity and probiotic composition as well as a reduction in Gram-negative Proteobacteria and Deferribacteres.
32955050	3	54	theme	adipose	556:562	arg1	tissue					564:569	the white adipose tissue	546:569	the white adipose tissue (WAT)	546:575	Hematoxylin/eosin staining, transmission electron microscopy, and immunohistochemical staining were used to evaluate histopathological alterations in the white adipose tissue (WAT) and/or jejunums.
32955050	6	55	theme	receptor	906:913	arg1	pathway					934:940	lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4) signaling pathway	871:940	lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4) signaling pathway in the WAT	871:951	Western blotting was employed to evaluate the relative protein expressions involved in lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4) signaling pathway in the WAT.
32955050	9	56	theme	microbiota	1625:1634	arg1	abundances					1636:1645	gut microbiota abundances	1621:1645	gut microbiota abundances	1621:1645	In summary, Sal B may attenuate body weight gain and insulin resistance through the regulation of gut microbiota abundances and LPS/TLR4 signaling pathway in obese mice, suggesting Sal B could be a promising drug candidate for protection against obesity.
32955050	7	57	from	reduction	1229:1237	arg1	integrity					1180:1188	intestinal epithelial integrity	1158:1188	intestinal epithelial integrity	1158:1188	Treatment of obese mice with Sal B improves insulin sensitivity, attenuates body weight gain and alleviates serum levels of LPS and tumor necrosis factor alpha, which is associated with an improvement in intestinal epithelial integrity and probiotic composition as well as a reduction in Gram-negative Proteobacteria and Deferribacteres.
32955050	7	57	from	reduction	1229:1237	arg1	Proteobacteria					1256:1269	Gram-negative Proteobacteria	1242:1269	Gram-negative Proteobacteria	1242:1269	Treatment of obese mice with Sal B improves insulin sensitivity, attenuates body weight gain and alleviates serum levels of LPS and tumor necrosis factor alpha, which is associated with an improvement in intestinal epithelial integrity and probiotic composition as well as a reduction in Gram-negative Proteobacteria and Deferribacteres.
32955050	7	57	from	reduction	1229:1237	arg1	composition					1204:1214	probiotic composition	1194:1214	probiotic composition	1194:1214	Treatment of obese mice with Sal B improves insulin sensitivity, attenuates body weight gain and alleviates serum levels of LPS and tumor necrosis factor alpha, which is associated with an improvement in intestinal epithelial integrity and probiotic composition as well as a reduction in Gram-negative Proteobacteria and Deferribacteres.
32955050	7	57	from	reduction	1229:1237	arg1	Deferribacteres					1275:1289	Deferribacteres	1275:1289	Deferribacteres	1275:1289	Treatment of obese mice with Sal B improves insulin sensitivity, attenuates body weight gain and alleviates serum levels of LPS and tumor necrosis factor alpha, which is associated with an improvement in intestinal epithelial integrity and probiotic composition as well as a reduction in Gram-negative Proteobacteria and Deferribacteres.
32955050	7	58	theme	LPS	1078:1080	arg1	levels					1068:1073	serum levels	1062:1073	serum levels of LPS and tumor necrosis factor alpha, which is associated with an improvement in intestinal epithelial integrity and probiotic composition as well as a reduction in Gram-negative Proteobacteria and Deferribacteres	1062:1289	Treatment of obese mice with Sal B improves insulin sensitivity, attenuates body weight gain and alleviates serum levels of LPS and tumor necrosis factor alpha, which is associated with an improvement in intestinal epithelial integrity and probiotic composition as well as a reduction in Gram-negative Proteobacteria and Deferribacteres.
32955050	9	59	from	regulation	1607:1616	arg1	mice					1687:1690	obese mice	1681:1690	obese mice	1681:1690	In summary, Sal B may attenuate body weight gain and insulin resistance through the regulation of gut microbiota abundances and LPS/TLR4 signaling pathway in obese mice, suggesting Sal B could be a promising drug candidate for protection against obesity.
32955050	9	60	theme	promising	1721:1729	arg1	candidate					1736:1744	a promising drug candidate	1719:1744	a promising drug candidate for protection against obesity	1719:1775	In summary, Sal B may attenuate body weight gain and insulin resistance through the regulation of gut microbiota abundances and LPS/TLR4 signaling pathway in obese mice, suggesting Sal B could be a promising drug candidate for protection against obesity.
32955050	9	60	theme	promising	1721:1729	arg1	B					1708:1708	Sal B	1704:1708	Sal B	1704:1708	In summary, Sal B may attenuate body weight gain and insulin resistance through the regulation of gut microbiota abundances and LPS/TLR4 signaling pathway in obese mice, suggesting Sal B could be a promising drug candidate for protection against obesity.
32955050	6	61	theme	Western	784:790	arg1	blotting					792:799	Western blotting	784:799	Western blotting	784:799	Western blotting was employed to evaluate the relative protein expressions involved in lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4) signaling pathway in the WAT.
32955050	4	62	from	synthesis	656:664	arg1	the					669:671	the	669:671	the	669:671	The expression levels of genes related to fat and cholesterol synthesis in the WAT were determined by qPCR.
32955050	9	63	theme	signaling	1660:1668	arg1	pathway					1670:1676	LPS/TLR4 signaling pathway	1651:1676	LPS/TLR4 signaling pathway	1651:1676	In summary, Sal B may attenuate body weight gain and insulin resistance through the regulation of gut microbiota abundances and LPS/TLR4 signaling pathway in obese mice, suggesting Sal B could be a promising drug candidate for protection against obesity.
32955050	0	64	theme	diet-induced	117:128	arg1	mice					136:139	high-fat diet-induced obese mice	108:139	high-fat diet-induced obese mice	108:139	Salvianolic acid B prevents body weight gain and regulates gut microbiota and LPS/TLR4 signaling pathway in high-fat diet-induced obese mice.
32955050	9	65	from	abundances	1636:1645	arg1	mice					1687:1690	obese mice	1681:1690	obese mice	1681:1690	In summary, Sal B may attenuate body weight gain and insulin resistance through the regulation of gut microbiota abundances and LPS/TLR4 signaling pathway in obese mice, suggesting Sal B could be a promising drug candidate for protection against obesity.
32955050	8	66	theme	kinase	1441:1446	arg1	levels					1416:1421	the phosphorylation levels	1396:1421	the phosphorylation levels of Jun N-terminal kinase, nuclear factor-kappa B p65, and an insulin receptor substrate	1396:1509	In addition, Sal B downregulates the expressions of TLR4 and myeloid differential factor-88, as well as the phosphorylation levels of Jun N-terminal kinase, nuclear factor-kappa B p65, and an insulin receptor substrate in the WAT.
32955050	8	66	theme	kinase	1441:1446	arg1	expressions					1329:1339	the expressions	1325:1339	the expressions of TLR4 and myeloid differential factor-88	1325:1382	In addition, Sal B downregulates the expressions of TLR4 and myeloid differential factor-88, as well as the phosphorylation levels of Jun N-terminal kinase, nuclear factor-kappa B p65, and an insulin receptor substrate in the WAT.
32955050	8	67	theme	nuclear	1449:1455	arg1	B					1470:1470	nuclear factor-kappa B	1449:1470	nuclear factor-kappa B p65	1449:1474	In addition, Sal B downregulates the expressions of TLR4 and myeloid differential factor-88, as well as the phosphorylation levels of Jun N-terminal kinase, nuclear factor-kappa B p65, and an insulin receptor substrate in the WAT.
32955050	5	68	theme	fecal	721:725	arg1	microbiota					727:736	fecal microbiota	721:736	fecal microbiota	721:736	The composition of fecal microbiota was profiled by 16S rRNA gene pyrosequencing.
32955050	7	69	theme	body	1030:1033	arg1	gain					1042:1045	body weight gain	1030:1045	body weight gain	1030:1045	Treatment of obese mice with Sal B improves insulin sensitivity, attenuates body weight gain and alleviates serum levels of LPS and tumor necrosis factor alpha, which is associated with an improvement in intestinal epithelial integrity and probiotic composition as well as a reduction in Gram-negative Proteobacteria and Deferribacteres.
32955050	7	70	with	Treatment	954:962	arg1	B					987:987	Sal B	983:987	Sal B	983:987	Treatment of obese mice with Sal B improves insulin sensitivity, attenuates body weight gain and alleviates serum levels of LPS and tumor necrosis factor alpha, which is associated with an improvement in intestinal epithelial integrity and probiotic composition as well as a reduction in Gram-negative Proteobacteria and Deferribacteres.
32955050	8	71	theme	B	1470:1470	arg1	p65					1472:1474	nuclear factor-kappa B p65	1449:1474	nuclear factor-kappa B p65	1449:1474	In addition, Sal B downregulates the expressions of TLR4 and myeloid differential factor-88, as well as the phosphorylation levels of Jun N-terminal kinase, nuclear factor-kappa B p65, and an insulin receptor substrate in the WAT.
32955050	0	72	theme	gut	59:61	arg1	microbiota					63:72	gut microbiota	59:72	gut microbiota	59:72	Salvianolic acid B prevents body weight gain and regulates gut microbiota and LPS/TLR4 signaling pathway in high-fat diet-induced obese mice.
32955050	7	73	theme	Sal	983:985	arg1	B					987:987	Sal B	983:987	Sal B	983:987	Treatment of obese mice with Sal B improves insulin sensitivity, attenuates body weight gain and alleviates serum levels of LPS and tumor necrosis factor alpha, which is associated with an improvement in intestinal epithelial integrity and probiotic composition as well as a reduction in Gram-negative Proteobacteria and Deferribacteres.
32955050	8	74	theme	Sal	1305:1307	arg1	B					1309:1309	Sal B	1305:1309	Sal B	1305:1309	In addition, Sal B downregulates the expressions of TLR4 and myeloid differential factor-88, as well as the phosphorylation levels of Jun N-terminal kinase, nuclear factor-kappa B p65, and an insulin receptor substrate in the WAT.
32955050	5	75	theme	microbiota	727:736	arg1	composition					706:716	The composition	702:716	The composition of fecal microbiota	702:736	The composition of fecal microbiota was profiled by 16S rRNA gene pyrosequencing.
32955050	5	76	theme	rRNA	758:761	arg1	pyrosequencing					768:781	rRNA gene pyrosequencing	758:781	rRNA gene pyrosequencing	758:781	The composition of fecal microbiota was profiled by 16S rRNA gene pyrosequencing.
32955050	7	77	theme	mice	973:976	arg1	Treatment					954:962	Treatment	954:962	Treatment of obese mice with Sal B	954:987	Treatment of obese mice with Sal B improves insulin sensitivity, attenuates body weight gain and alleviates serum levels of LPS and tumor necrosis factor alpha, which is associated with an improvement in intestinal epithelial integrity and probiotic composition as well as a reduction in Gram-negative Proteobacteria and Deferribacteres.
32955050	1	78	theme	acid	154:157	arg1	B					166:166	Sal B	162:166	Sal B	162:166	Salvianolic acid B (Sal B) exhibits anti-obesity activity, yet the underlying mechanism linking this effect to metabolic endotoxemia remains unexplored.
32955050	1	78	theme	acid	154:157	arg1	B					159:159	Salvianolic acid B	142:159	Salvianolic acid B (Sal B)	142:167	Salvianolic acid B (Sal B) exhibits anti-obesity activity, yet the underlying mechanism linking this effect to metabolic endotoxemia remains unexplored.
32955050	0	79	theme	high-fat	108:115	arg1	mice					136:139	high-fat diet-induced obese mice	108:139	high-fat diet-induced obese mice	108:139	Salvianolic acid B prevents body weight gain and regulates gut microbiota and LPS/TLR4 signaling pathway in high-fat diet-induced obese mice.
32955050	8	80	theme	receptor	1492:1499	arg1	substrate					1501:1509	an insulin receptor substrate	1481:1509	an insulin receptor substrate	1481:1509	In addition, Sal B downregulates the expressions of TLR4 and myeloid differential factor-88, as well as the phosphorylation levels of Jun N-terminal kinase, nuclear factor-kappa B p65, and an insulin receptor substrate in the WAT.
32955050	0	81	theme	signaling	87:95	arg1	pathway					97:103	LPS/TLR4 signaling pathway	78:103	LPS/TLR4 signaling pathway	78:103	Salvianolic acid B prevents body weight gain and regulates gut microbiota and LPS/TLR4 signaling pathway in high-fat diet-induced obese mice.
32955050	9	82	from	pathway	1670:1676	arg1	mice					1687:1690	obese mice	1681:1690	obese mice	1681:1690	In summary, Sal B may attenuate body weight gain and insulin resistance through the regulation of gut microbiota abundances and LPS/TLR4 signaling pathway in obese mice, suggesting Sal B could be a promising drug candidate for protection against obesity.
32955050	7	83	theme	intestinal	1158:1167	arg1	integrity					1180:1188	intestinal epithelial integrity	1158:1188	intestinal epithelial integrity	1158:1188	Treatment of obese mice with Sal B improves insulin sensitivity, attenuates body weight gain and alleviates serum levels of LPS and tumor necrosis factor alpha, which is associated with an improvement in intestinal epithelial integrity and probiotic composition as well as a reduction in Gram-negative Proteobacteria and Deferribacteres.
32955050	7	84	from	improvement	1143:1153	arg1	integrity					1180:1188	intestinal epithelial integrity	1158:1188	intestinal epithelial integrity	1158:1188	Treatment of obese mice with Sal B improves insulin sensitivity, attenuates body weight gain and alleviates serum levels of LPS and tumor necrosis factor alpha, which is associated with an improvement in intestinal epithelial integrity and probiotic composition as well as a reduction in Gram-negative Proteobacteria and Deferribacteres.
32955050	7	84	from	improvement	1143:1153	arg1	Proteobacteria					1256:1269	Gram-negative Proteobacteria	1242:1269	Gram-negative Proteobacteria	1242:1269	Treatment of obese mice with Sal B improves insulin sensitivity, attenuates body weight gain and alleviates serum levels of LPS and tumor necrosis factor alpha, which is associated with an improvement in intestinal epithelial integrity and probiotic composition as well as a reduction in Gram-negative Proteobacteria and Deferribacteres.
32955050	7	84	from	improvement	1143:1153	arg1	composition					1204:1214	probiotic composition	1194:1214	probiotic composition	1194:1214	Treatment of obese mice with Sal B improves insulin sensitivity, attenuates body weight gain and alleviates serum levels of LPS and tumor necrosis factor alpha, which is associated with an improvement in intestinal epithelial integrity and probiotic composition as well as a reduction in Gram-negative Proteobacteria and Deferribacteres.
32955050	7	84	from	improvement	1143:1153	arg1	Deferribacteres					1275:1289	Deferribacteres	1275:1289	Deferribacteres	1275:1289	Treatment of obese mice with Sal B improves insulin sensitivity, attenuates body weight gain and alleviates serum levels of LPS and tumor necrosis factor alpha, which is associated with an improvement in intestinal epithelial integrity and probiotic composition as well as a reduction in Gram-negative Proteobacteria and Deferribacteres.
32955050	8	85	theme	TLR4	1344:1347	arg1	levels					1416:1421	the phosphorylation levels	1396:1421	the phosphorylation levels of Jun N-terminal kinase, nuclear factor-kappa B p65, and an insulin receptor substrate	1396:1509	In addition, Sal B downregulates the expressions of TLR4 and myeloid differential factor-88, as well as the phosphorylation levels of Jun N-terminal kinase, nuclear factor-kappa B p65, and an insulin receptor substrate in the WAT.
32955050	8	85	theme	TLR4	1344:1347	arg1	expressions					1329:1339	the expressions	1325:1339	the expressions of TLR4 and myeloid differential factor-88	1325:1382	In addition, Sal B downregulates the expressions of TLR4 and myeloid differential factor-88, as well as the phosphorylation levels of Jun N-terminal kinase, nuclear factor-kappa B p65, and an insulin receptor substrate in the WAT.
32955050	3	86	theme	electron	437:444	arg1	microscopy					446:455	transmission electron microscopy	424:455	transmission electron microscopy	424:455	Hematoxylin/eosin staining, transmission electron microscopy, and immunohistochemical staining were used to evaluate histopathological alterations in the white adipose tissue (WAT) and/or jejunums.
32955050	0	87	theme	acid	12:15	arg1	B					17:17	Salvianolic acid B	0:17	Salvianolic acid B	0:17	Salvianolic acid B prevents body weight gain and regulates gut microbiota and LPS/TLR4 signaling pathway in high-fat diet-induced obese mice.
32955050	9	88	theme	Sal	1535:1537	arg1	B					1539:1539	Sal B	1535:1539	Sal B	1535:1539	In summary, Sal B may attenuate body weight gain and insulin resistance through the regulation of gut microbiota abundances and LPS/TLR4 signaling pathway in obese mice, suggesting Sal B could be a promising drug candidate for protection against obesity.
32955050	8	89	theme	myeloid	1353:1359	arg1	factor-88					1374:1382	myeloid differential factor-88	1353:1382	myeloid differential factor-88	1353:1382	In addition, Sal B downregulates the expressions of TLR4 and myeloid differential factor-88, as well as the phosphorylation levels of Jun N-terminal kinase, nuclear factor-kappa B p65, and an insulin receptor substrate in the WAT.
32955050	0	90	from	microbiota	63:72	arg1	mice					136:139	high-fat diet-induced obese mice	108:139	high-fat diet-induced obese mice	108:139	Salvianolic acid B prevents body weight gain and regulates gut microbiota and LPS/TLR4 signaling pathway in high-fat diet-induced obese mice.
32955050	8	91	theme	factor-88	1374:1382	arg1	levels					1416:1421	the phosphorylation levels	1396:1421	the phosphorylation levels of Jun N-terminal kinase, nuclear factor-kappa B p65, and an insulin receptor substrate	1396:1509	In addition, Sal B downregulates the expressions of TLR4 and myeloid differential factor-88, as well as the phosphorylation levels of Jun N-terminal kinase, nuclear factor-kappa B p65, and an insulin receptor substrate in the WAT.
32955050	8	91	theme	factor-88	1374:1382	arg1	expressions					1329:1339	the expressions	1325:1339	the expressions of TLR4 and myeloid differential factor-88	1325:1382	In addition, Sal B downregulates the expressions of TLR4 and myeloid differential factor-88, as well as the phosphorylation levels of Jun N-terminal kinase, nuclear factor-kappa B p65, and an insulin receptor substrate in the WAT.
32955050	3	92	theme	immunohistochemical	462:480	arg1	staining					482:489	immunohistochemical staining	462:489	immunohistochemical staining	462:489	Hematoxylin/eosin staining, transmission electron microscopy, and immunohistochemical staining were used to evaluate histopathological alterations in the white adipose tissue (WAT) and/or jejunums.
32955050	0	93	theme	weight	33:38	arg1	gain					40:43	body weight gain	28:43	body weight gain	28:43	Salvianolic acid B prevents body weight gain and regulates gut microbiota and LPS/TLR4 signaling pathway in high-fat diet-induced obese mice.
32955050	6	94	theme	lipopolysaccharide	871:888	arg1	pathway					934:940	lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4) signaling pathway	871:940	lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4) signaling pathway in the WAT	871:951	Western blotting was employed to evaluate the relative protein expressions involved in lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4) signaling pathway in the WAT.
32955050	9	95	theme	body	1555:1558	arg1	gain					1567:1570	body weight gain	1555:1570	body weight gain	1555:1570	In summary, Sal B may attenuate body weight gain and insulin resistance through the regulation of gut microbiota abundances and LPS/TLR4 signaling pathway in obese mice, suggesting Sal B could be a promising drug candidate for protection against obesity.
32955050	3	96	theme	Hematoxylin/eosin	396:412	arg1	staining					414:421	Hematoxylin/eosin staining	396:421	Hematoxylin/eosin staining	396:421	Hematoxylin/eosin staining, transmission electron microscopy, and immunohistochemical staining were used to evaluate histopathological alterations in the white adipose tissue (WAT) and/or jejunums.
32955050	3	97	used	used	496:499	arg2	staining					414:421	Hematoxylin/eosin staining	396:421	Hematoxylin/eosin staining	396:421	Hematoxylin/eosin staining, transmission electron microscopy, and immunohistochemical staining were used to evaluate histopathological alterations in the white adipose tissue (WAT) and/or jejunums.
32955050	3	97	used	used	496:499	arg2	microscopy					446:455	transmission electron microscopy	424:455	transmission electron microscopy	424:455	Hematoxylin/eosin staining, transmission electron microscopy, and immunohistochemical staining were used to evaluate histopathological alterations in the white adipose tissue (WAT) and/or jejunums.
32955050	3	97	used	used	496:499	arg2	staining					482:489	immunohistochemical staining	462:489	immunohistochemical staining	462:489	Hematoxylin/eosin staining, transmission electron microscopy, and immunohistochemical staining were used to evaluate histopathological alterations in the white adipose tissue (WAT) and/or jejunums.
32955050	2	98	theme	diet-induced	322:333	arg1	mice					341:344	high-fat diet-induced obese mice	313:344	high-fat diet-induced obese mice	313:344	For this purpose, high-fat diet-induced obese mice were orally administered with Sal B for 10 weeks.
32955050	6	99	theme	protein	839:845	arg1	expressions					847:857	the relative protein expressions	826:857	the relative protein expressions involved in lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4) signaling pathway in the WAT	826:951	Western blotting was employed to evaluate the relative protein expressions involved in lipopolysaccharide (LPS)/toll-like receptor 4 (TLR4) signaling pathway in the WAT.
32955050	9	100	theme	insulin	1576:1582	arg1	resistance					1584:1593	insulin resistance	1576:1593	insulin resistance	1576:1593	In summary, Sal B may attenuate body weight gain and insulin resistance through the regulation of gut microbiota abundances and LPS/TLR4 signaling pathway in obese mice, suggesting Sal B could be a promising drug candidate for protection against obesity.
32955050	7	101	theme	Gram-negative	1242:1254	arg1	Proteobacteria					1256:1269	Gram-negative Proteobacteria	1242:1269	Gram-negative Proteobacteria	1242:1269	Treatment of obese mice with Sal B improves insulin sensitivity, attenuates body weight gain and alleviates serum levels of LPS and tumor necrosis factor alpha, which is associated with an improvement in intestinal epithelial integrity and probiotic composition as well as a reduction in Gram-negative Proteobacteria and Deferribacteres.
32955050	8	102	theme	phosphorylation	1400:1414	arg1	levels					1416:1421	the phosphorylation levels	1396:1421	the phosphorylation levels of Jun N-terminal kinase, nuclear factor-kappa B p65, and an insulin receptor substrate	1396:1509	In addition, Sal B downregulates the expressions of TLR4 and myeloid differential factor-88, as well as the phosphorylation levels of Jun N-terminal kinase, nuclear factor-kappa B p65, and an insulin receptor substrate in the WAT.
32955050	4	103	theme	related	625:631	arg1	genes					619:623	genes	619:623	genes related to fat and cholesterol synthesis in the	619:671	The expression levels of genes related to fat and cholesterol synthesis in the WAT were determined by qPCR.
32870484	9	0	from	level	1939:1943	arg1	mucosa					1991:1996	cecal mucosa	1985:1996	cecal mucosa induced by E. coli infection (P < 0.05)	1985:2036	In addition, CS-Zn supplementation significantly prevented the elevation of pro-inflammatory cytokines IL-6 concentration and up-regulated the level of anti-inflammatory cytokines IL-10 in cecal mucosa induced by E. coli infection (P < 0.05).
32870484	2	1	theme	study	340:344	arg1	objective					322:330	the objective	318:330	the objective of this study	318:344	Therefore, the objective of this study was to evaluate whether chitosan-chelated zinc (CS-Zn) supplementation could attenuate gut injury induced by E. coli challenge and to explore how CS-Zn modulates cecal microbiota and alleviates intestinal inflammation in weaned rats challenged with E. coli.
32870484	9	2	dep	cytokines	1889:1897	arg1	concentration					1904:1916	IL-6 concentration	1899:1916	pro-inflammatory cytokines IL-6 concentration	1872:1916	In addition, CS-Zn supplementation significantly prevented the elevation of pro-inflammatory cytokines IL-6 concentration and up-regulated the level of anti-inflammatory cytokines IL-10 in cecal mucosa induced by E. coli infection (P < 0.05).
32870484	3	3	dep	CS-Zn	849:853	arg1	containing					873:882	containing	873:882	containing 50 mg/kg Zn	873:894	36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
32870484	6	4	dep	E.	1387:1388	arg1	coli					1390:1393	coli	1390:1393	coli	1390:1393	16S rRNA sequencing analyses revealed that E. coli challenge significantly increased the abundance of Verrucomicrobia and E. coli (P < 0.05).
32870484	3	5	theme	mg/kg	887:891	arg1	Zn					893:894	50 mg/kg Zn	884:894	50 mg/kg Zn	884:894	36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
32870484	5	6	theme	coli	1219:1222	arg1	challenge					1224:1232	E. coli challenge	1216:1232	E. coli challenge	1216:1232	CS-Zn supplementation mitigated intestinal morphology impairment (e.g. higher crypt depth and lower macroscopic damage index) induced by E. coli challenge (P < 0.05), and alleviated the increase of Myeloperoxidase (MPO) activity after E. coli challenge (P < 0.05).
32870484	3	7	dep	E.	856:857	arg1	coli					859:862	E. coli	856:862	E. coli	856:862	36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
32870484	4	8	theme	E.	967:968	arg1	solution					975:982	E. coli solution	967:982	E. coli solution	967:982	On days 10 to 12, each rat was given 4 ml of E. coli solution with a total bacteria count of 1010 CFU by oral gavage daily or normal saline of equal dosage.
32870484	9	9	theme	pro-inflammatory	1872:1887	arg1	cytokines					1889:1897	pro-inflammatory cytokines IL-6 concentration	1872:1916	pro-inflammatory cytokines IL-6 concentration	1872:1916	In addition, CS-Zn supplementation significantly prevented the elevation of pro-inflammatory cytokines IL-6 concentration and up-regulated the level of anti-inflammatory cytokines IL-10 in cecal mucosa induced by E. coli infection (P < 0.05).
32870484	3	10	contain	containing	828:837	arg2	CS-Zn					849:853	640 mg/kg CS-Zn	839:853	640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment	839:919	36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
32870484	3	10	contain	containing	828:837	arg1	diet					823:826	a diet	821:826	a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment	821:919	36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
32870484	10	11	theme	bacterial	2237:2245	arg1	composition					2247:2257	cecal bacterial composition	2231:2257	cecal bacterial composition	2231:2257	In conclusion, these results indicate that CS-Zn produces beneficial effects in alleviating gut mucosal injury of E. coli challenged rats by enhancing the intestinal morphology and modulating cecal bacterial composition, as well as attenuating inflammatory response.
32870484	9	12	theme	IL-6	1899:1902	arg1	concentration					1904:1916	IL-6 concentration	1899:1916	pro-inflammatory cytokines IL-6 concentration	1872:1916	In addition, CS-Zn supplementation significantly prevented the elevation of pro-inflammatory cytokines IL-6 concentration and up-regulated the level of anti-inflammatory cytokines IL-10 in cecal mucosa induced by E. coli infection (P < 0.05).
32870484	1	13	theme	growing	200:206	arg1	animals					208:214	young growing animals	194:214	young growing animals	194:214	Escherichia coli (E. coli) infection is very common among young growing animals, and zinc supplementation is often used to alleviate inflammation induced by this disease.
32870484	8	14	theme	butyrate	1674:1681	arg1	concentrations					1656:1669	The concentrations	1652:1669	The concentrations of butyrate in the cecal digesta, which decreased due to the challenge,	1652:1741	The concentrations of butyrate in the cecal digesta, which decreased due to the challenge, were higher in the E. coli + CS-Zn group (P < 0.05).
32870484	8	14	theme	butyrate	1674:1681	arg1	higher					1748:1753	higher	1748:1753	higher	1748:1753	The concentrations of butyrate in the cecal digesta, which decreased due to the challenge, were higher in the E. coli + CS-Zn group (P < 0.05).
32870484	8	15	theme	E.	1762:1763	arg1	<					1787:1787	P < 0.05	1785:1792	P < 0.05	1785:1792	The concentrations of butyrate in the cecal digesta, which decreased due to the challenge, were higher in the E. coli + CS-Zn group (P < 0.05).
32870484	8	15	theme	E.	1762:1763	arg1	group					1778:1782	the E. coli + CS-Zn group	1758:1782	the E. coli + CS-Zn group (P < 0.05)	1758:1793	The concentrations of butyrate in the cecal digesta, which decreased due to the challenge, were higher in the E. coli + CS-Zn group (P < 0.05).
32870484	8	16	dep	E.	1762:1763	arg1	coli					1765:1768	coli	1765:1768	coli	1765:1768	The concentrations of butyrate in the cecal digesta, which decreased due to the challenge, were higher in the E. coli + CS-Zn group (P < 0.05).
32870484	0	17	from	response	78:85	arg1	rats					97:100	weaned rats	90:100	weaned rats challenged with Escherichia coli	90:133	Chitosan-chelated zinc modulates cecal microbiota and attenuates inflammatory response in weaned rats challenged with Escherichia coli.
32870484	4	18	theme	bacteria	997:1004	arg1	count					1006:1010	a total bacteria count	989:1010	a total bacteria count of 1010 CFU	989:1022	On days 10 to 12, each rat was given 4 ml of E. coli solution with a total bacteria count of 1010 CFU by oral gavage daily or normal saline of equal dosage.
32870484	6	19	theme	rRNA	1348:1351	arg1	analyses					1364:1371	16S rRNA sequencing analyses	1344:1371	16S rRNA sequencing analyses	1344:1371	16S rRNA sequencing analyses revealed that E. coli challenge significantly increased the abundance of Verrucomicrobia and E. coli (P < 0.05).
32870484	7	20	theme	CS-Zn	1495:1499	arg1	supplementation					1501:1515	CS-Zn supplementation	1495:1515	CS-Zn supplementation	1495:1515	However, CS-Zn supplementation increased the abundance of Lactobacillus and decreased the relative abundance of Proteobacteria, Desulfovibrio and E. coli (P < 0.05).
32870484	2	21	theme	weaned	567:572	arg1	rats					574:577	weaned rats	567:577	weaned rats challenged with E. coli	567:601	Therefore, the objective of this study was to evaluate whether chitosan-chelated zinc (CS-Zn) supplementation could attenuate gut injury induced by E. coli challenge and to explore how CS-Zn modulates cecal microbiota and alleviates intestinal inflammation in weaned rats challenged with E. coli.
32870484	6	22	theme	Verrucomicrobia	1446:1460	arg1	abundance					1433:1441	the abundance	1429:1441	the abundance of Verrucomicrobia and E. coli (P < 0.05)	1429:1483	16S rRNA sequencing analyses revealed that E. coli challenge significantly increased the abundance of Verrucomicrobia and E. coli (P < 0.05).
32870484	3	23	theme	rats	768:771	arg1	Control					741:747	Control	741:747	Control	741:747	36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
32870484	3	23	theme	rats	768:771	arg1	diet					735:738	a basal diet	727:738	a basal diet (Control)	727:748	36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
32870484	3	23	theme	rats	768:771	arg1	groups					758:763	two groups	754:763	two groups of rats challenged with E. coli	754:795	36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
32870484	3	23	theme	rats	768:771	arg1	rats					768:771	rats	768:771	rats challenged with E. coli	768:795	36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
32870484	10	24	theme	beneficial	2097:2106	arg1	effects					2108:2114	beneficial effects	2097:2114	beneficial effects	2097:2114	In conclusion, these results indicate that CS-Zn produces beneficial effects in alleviating gut mucosal injury of E. coli challenged rats by enhancing the intestinal morphology and modulating cecal bacterial composition, as well as attenuating inflammatory response.
32870484	4	25	theme	solution	975:982	arg1	ml					961:962	4 ml	959:962	4 ml of E. coli solution with a total bacteria count of 1010 CFU	959:1022	On days 10 to 12, each rat was given 4 ml of E. coli solution with a total bacteria count of 1010 CFU by oral gavage daily or normal saline of equal dosage.
32870484	5	26	theme	intestinal	1111:1120	arg1	impairment					1133:1142	intestinal morphology impairment	1111:1142	intestinal morphology impairment (e.g. higher crypt depth and lower macroscopic damage index)	1111:1203	CS-Zn supplementation mitigated intestinal morphology impairment (e.g. higher crypt depth and lower macroscopic damage index) induced by E. coli challenge (P < 0.05), and alleviated the increase of Myeloperoxidase (MPO) activity after E. coli challenge (P < 0.05).
32870484	9	27	theme	cytokines	1966:1974	arg1	level					1939:1943	the level	1935:1943	the level of anti-inflammatory cytokines IL-10 in cecal mucosa induced by E. coli infection (P < 0.05)	1935:2036	In addition, CS-Zn supplementation significantly prevented the elevation of pro-inflammatory cytokines IL-6 concentration and up-regulated the level of anti-inflammatory cytokines IL-10 in cecal mucosa induced by E. coli infection (P < 0.05).
32870484	3	28	theme	weaned	607:612	arg1	rats					614:617	36 weaned rats	604:617	36 weaned rats (55.65 ± 2.18 g of BW, n = 12)	604:648	36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
32870484	0	29	theme	weaned	90:95	arg1	rats					97:100	weaned rats	90:100	weaned rats challenged with Escherichia coli	90:133	Chitosan-chelated zinc modulates cecal microbiota and attenuates inflammatory response in weaned rats challenged with Escherichia coli.
32870484	10	30	theme	gut	2131:2133	arg1	injury					2143:2148	gut mucosal injury	2131:2148	gut mucosal injury of E. coli challenged rats	2131:2175	In conclusion, these results indicate that CS-Zn produces beneficial effects in alleviating gut mucosal injury of E. coli challenged rats by enhancing the intestinal morphology and modulating cecal bacterial composition, as well as attenuating inflammatory response.
32870484	7	31	theme	Lactobacillus	1544:1556	arg1	abundance					1531:1539	the abundance	1527:1539	the abundance of Lactobacillus	1527:1556	However, CS-Zn supplementation increased the abundance of Lactobacillus and decreased the relative abundance of Proteobacteria, Desulfovibrio and E. coli (P < 0.05).
32870484	1	32	dep	coli	148:151	arg1	coli					157:160	E. coli	154:160	E. coli	154:160	Escherichia coli (E. coli) infection is very common among young growing animals, and zinc supplementation is often used to alleviate inflammation induced by this disease.
32870484	3	33	dep	rats	718:721	arg1	fed					723:725	fed	723:725	fed a basal diet (Control) and two groups of rats challenged with E. coli	723:795	36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
32870484	3	33	dep	rats	718:721	arg1	fed					801:803	fed	801:803	fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment	801:919	36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
32870484	4	34	theme	normal	1048:1053	arg1	saline					1055:1060	normal saline	1048:1060	normal saline of equal dosage	1048:1076	On days 10 to 12, each rat was given 4 ml of E. coli solution with a total bacteria count of 1010 CFU by oral gavage daily or normal saline of equal dosage.
32870484	5	35	theme	crypt	1157:1161	arg1	depth					1163:1167	higher crypt depth	1150:1167	higher crypt depth	1150:1167	CS-Zn supplementation mitigated intestinal morphology impairment (e.g. higher crypt depth and lower macroscopic damage index) induced by E. coli challenge (P < 0.05), and alleviated the increase of Myeloperoxidase (MPO) activity after E. coli challenge (P < 0.05).
32870484	3	36	theme	basal	807:811	arg1	diet					813:816	a basal diet	805:816	a basal diet	805:816	36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
32870484	4	37	theme	CFU	1020:1022	arg1	count					1006:1010	a total bacteria count	989:1010	a total bacteria count of 1010 CFU	989:1022	On days 10 to 12, each rat was given 4 ml of E. coli solution with a total bacteria count of 1010 CFU by oral gavage daily or normal saline of equal dosage.
32870484	2	38	theme	chitosan-chelated	370:386	arg1	supplementation					401:415	chitosan-chelated zinc (CS-Zn) supplementation	370:415	chitosan-chelated zinc (CS-Zn) supplementation	370:415	Therefore, the objective of this study was to evaluate whether chitosan-chelated zinc (CS-Zn) supplementation could attenuate gut injury induced by E. coli challenge and to explore how CS-Zn modulates cecal microbiota and alleviates intestinal inflammation in weaned rats challenged with E. coli.
32870484	0	39	theme	Chitosan-chelated	0:16	arg1	zinc					18:21	Chitosan-chelated zinc	0:21	Chitosan-chelated zinc	0:21	Chitosan-chelated zinc modulates cecal microbiota and attenuates inflammatory response in weaned rats challenged with Escherichia coli.
32870484	3	40	dep	=	644:644	arg1	g					633:633	55.65 ± 2.18 g	620:633	55.65 ± 2.18 g of BW	620:639	36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
32870484	3	41	theme	640	839:841	arg1	mg/kg					843:847	mg/kg	843:847	mg/kg	843:847	36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
32870484	2	42	theme	gut	433:435	arg1	injury					437:442	gut injury	433:442	gut injury induced by E. coli challenge	433:471	Therefore, the objective of this study was to evaluate whether chitosan-chelated zinc (CS-Zn) supplementation could attenuate gut injury induced by E. coli challenge and to explore how CS-Zn modulates cecal microbiota and alleviates intestinal inflammation in weaned rats challenged with E. coli.
32870484	5	43	theme	coli	1317:1320	arg1	challenge					1322:1330	E. coli challenge	1314:1330	E. coli challenge	1314:1330	CS-Zn supplementation mitigated intestinal morphology impairment (e.g. higher crypt depth and lower macroscopic damage index) induced by E. coli challenge (P < 0.05), and alleviated the increase of Myeloperoxidase (MPO) activity after E. coli challenge (P < 0.05).
32870484	4	44	theme	oral	1027:1030	arg1	gavage					1032:1037	oral gavage	1027:1037	oral gavage daily	1027:1043	On days 10 to 12, each rat was given 4 ml of E. coli solution with a total bacteria count of 1010 CFU by oral gavage daily or normal saline of equal dosage.
32870484	9	45	dep	cytokines	1966:1974	arg1	IL-10					1976:1980	IL-10	1976:1980	anti-inflammatory cytokines IL-10	1948:1980	In addition, CS-Zn supplementation significantly prevented the elevation of pro-inflammatory cytokines IL-6 concentration and up-regulated the level of anti-inflammatory cytokines IL-10 in cecal mucosa induced by E. coli infection (P < 0.05).
32870484	4	46	with	ml	961:962	arg1	count					1006:1010	a total bacteria count	989:1010	a total bacteria count of 1010 CFU	989:1022	On days 10 to 12, each rat was given 4 ml of E. coli solution with a total bacteria count of 1010 CFU by oral gavage daily or normal saline of equal dosage.
32870484	1	47	used	used	251:254	arg2	supplementation					226:240	zinc supplementation	221:240	zinc supplementation	221:240	Escherichia coli (E. coli) infection is very common among young growing animals, and zinc supplementation is often used to alleviate inflammation induced by this disease.
32870484	5	48	dep	depth	1163:1167	arg1	e.g.					1145:1148	e.g.	1145:1148	e.g.	1145:1148	CS-Zn supplementation mitigated intestinal morphology impairment (e.g. higher crypt depth and lower macroscopic damage index) induced by E. coli challenge (P < 0.05), and alleviated the increase of Myeloperoxidase (MPO) activity after E. coli challenge (P < 0.05).
32870484	2	49	theme	E.	455:456	arg1	challenge					463:471	E. coli challenge	455:471	E. coli challenge	455:471	Therefore, the objective of this study was to evaluate whether chitosan-chelated zinc (CS-Zn) supplementation could attenuate gut injury induced by E. coli challenge and to explore how CS-Zn modulates cecal microbiota and alleviates intestinal inflammation in weaned rats challenged with E. coli.
32870484	3	50	theme	±	626:626	arg1	g					633:633	55.65 ± 2.18 g	620:633	55.65 ± 2.18 g of BW	620:639	36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
32870484	8	51	theme	CS-Zn	1772:1776	arg1	<					1787:1787	P < 0.05	1785:1792	P < 0.05	1785:1792	The concentrations of butyrate in the cecal digesta, which decreased due to the challenge, were higher in the E. coli + CS-Zn group (P < 0.05).
32870484	8	51	theme	CS-Zn	1772:1776	arg1	group					1778:1782	the E. coli + CS-Zn group	1758:1782	the E. coli + CS-Zn group (P < 0.05)	1758:1793	The concentrations of butyrate in the cecal digesta, which decreased due to the challenge, were higher in the E. coli + CS-Zn group (P < 0.05).
32870484	5	52	theme	E.	1216:1217	arg1	challenge					1224:1232	E. coli challenge	1216:1232	E. coli challenge	1216:1232	CS-Zn supplementation mitigated intestinal morphology impairment (e.g. higher crypt depth and lower macroscopic damage index) induced by E. coli challenge (P < 0.05), and alleviated the increase of Myeloperoxidase (MPO) activity after E. coli challenge (P < 0.05).
32870484	4	53	theme	dosage	1071:1076	arg1	gavage					1032:1037	oral gavage	1027:1037	oral gavage daily	1027:1043	On days 10 to 12, each rat was given 4 ml of E. coli solution with a total bacteria count of 1010 CFU by oral gavage daily or normal saline of equal dosage.
32870484	4	53	theme	dosage	1071:1076	arg1	saline					1055:1060	normal saline	1048:1060	normal saline of equal dosage	1048:1076	On days 10 to 12, each rat was given 4 ml of E. coli solution with a total bacteria count of 1010 CFU by oral gavage daily or normal saline of equal dosage.
32870484	5	54	theme	macroscopic	1179:1189	arg1	index					1198:1202	lower macroscopic damage index	1173:1202	lower macroscopic damage index	1173:1202	CS-Zn supplementation mitigated intestinal morphology impairment (e.g. higher crypt depth and lower macroscopic damage index) induced by E. coli challenge (P < 0.05), and alleviated the increase of Myeloperoxidase (MPO) activity after E. coli challenge (P < 0.05).
32870484	10	55	theme	challenged	2161:2170	arg1	rats					2172:2175	E. coli challenged rats	2153:2175	E. coli challenged rats	2153:2175	In conclusion, these results indicate that CS-Zn produces beneficial effects in alleviating gut mucosal injury of E. coli challenged rats by enhancing the intestinal morphology and modulating cecal bacterial composition, as well as attenuating inflammatory response.
32870484	7	56	theme	relative	1576:1583	arg1	abundance					1585:1593	the relative abundance	1572:1593	the relative abundance of Proteobacteria, Desulfovibrio and E. coli (P < 0.05)	1572:1649	However, CS-Zn supplementation increased the abundance of Lactobacillus and decreased the relative abundance of Proteobacteria, Desulfovibrio and E. coli (P < 0.05).
32870484	8	57	from	concentrations	1656:1669	arg1	digesta					1696:1702	the cecal digesta	1686:1702	the cecal digesta	1686:1702	The concentrations of butyrate in the cecal digesta, which decreased due to the challenge, were higher in the E. coli + CS-Zn group (P < 0.05).
32870484	9	58	theme	CS-Zn	1809:1813	arg1	supplementation					1815:1829	CS-Zn supplementation	1809:1829	CS-Zn supplementation	1809:1829	In addition, CS-Zn supplementation significantly prevented the elevation of pro-inflammatory cytokines IL-6 concentration and up-regulated the level of anti-inflammatory cytokines IL-10 in cecal mucosa induced by E. coli infection (P < 0.05).
32870484	3	59	theme	50	884:885	arg1	mg/kg					887:891	mg/kg	887:891	mg/kg	887:891	36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
32870484	3	60	theme	treatment	674:682	arg1	groups					684:689	three treatment groups	668:689	three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment	668:919	36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
32870484	3	61	theme	14-day	903:908	arg1	experiment					910:919	a 14-day experiment	901:919	a 14-day experiment	901:919	36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
32870484	4	62	theme	coli	970:973	arg1	solution					975:982	E. coli solution	967:982	E. coli solution	967:982	On days 10 to 12, each rat was given 4 ml of E. coli solution with a total bacteria count of 1010 CFU by oral gavage daily or normal saline of equal dosage.
32870484	10	63	theme	intestinal	2194:2203	arg1	morphology					2205:2214	the intestinal morphology	2190:2214	the intestinal morphology	2190:2214	In conclusion, these results indicate that CS-Zn produces beneficial effects in alleviating gut mucosal injury of E. coli challenged rats by enhancing the intestinal morphology and modulating cecal bacterial composition, as well as attenuating inflammatory response.
32870484	10	64	theme	rats	2172:2175	arg1	injury					2143:2148	gut mucosal injury	2131:2148	gut mucosal injury of E. coli challenged rats	2131:2175	In conclusion, these results indicate that CS-Zn produces beneficial effects in alleviating gut mucosal injury of E. coli challenged rats by enhancing the intestinal morphology and modulating cecal bacterial composition, as well as attenuating inflammatory response.
32870484	3	65	theme	unchallenged	705:716	arg1	rats					718:721	unchallenged rats	705:721	unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment	705:919	36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
32870484	5	66	theme	P	1235:1235	arg1	<					1237:1237	P < 0.05	1235:1242	P < 0.05	1235:1242	CS-Zn supplementation mitigated intestinal morphology impairment (e.g. higher crypt depth and lower macroscopic damage index) induced by E. coli challenge (P < 0.05), and alleviated the increase of Myeloperoxidase (MPO) activity after E. coli challenge (P < 0.05).
32870484	1	67	theme	young	194:198	arg1	animals					208:214	young growing animals	194:214	young growing animals	194:214	Escherichia coli (E. coli) infection is very common among young growing animals, and zinc supplementation is often used to alleviate inflammation induced by this disease.
32870484	2	68	theme	intestinal	540:549	arg1	inflammation					551:562	intestinal inflammation	540:562	intestinal inflammation in weaned rats challenged with E. coli	540:601	Therefore, the objective of this study was to evaluate whether chitosan-chelated zinc (CS-Zn) supplementation could attenuate gut injury induced by E. coli challenge and to explore how CS-Zn modulates cecal microbiota and alleviates intestinal inflammation in weaned rats challenged with E. coli.
32870484	6	69	theme	P	1475:1475	arg1	coli					1469:1472	E. coli	1466:1472	E. coli (P < 0.05)	1466:1483	16S rRNA sequencing analyses revealed that E. coli challenge significantly increased the abundance of Verrucomicrobia and E. coli (P < 0.05).
32870484	6	69	theme	P	1475:1475	arg1	<					1477:1477	P < 0.05	1475:1482	P < 0.05	1475:1482	16S rRNA sequencing analyses revealed that E. coli challenge significantly increased the abundance of Verrucomicrobia and E. coli (P < 0.05).
32870484	10	70	theme	cecal	2231:2235	arg1	composition					2247:2257	cecal bacterial composition	2231:2257	cecal bacterial composition	2231:2257	In conclusion, these results indicate that CS-Zn produces beneficial effects in alleviating gut mucosal injury of E. coli challenged rats by enhancing the intestinal morphology and modulating cecal bacterial composition, as well as attenuating inflammatory response.
32870484	3	71	dep	containing	873:882	arg1	E.					856:857	E.	856:857	E.	856:857	36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
32870484	3	71	dep	containing	873:882	arg1	CS-Zn					866:870	CS-Zn	866:870	CS-Zn	866:870	36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
32870484	3	72	dep	rats	614:617	arg1	=					644:644	=	644:644	=	644:644	36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
32870484	6	73	theme	coli	1469:1472	arg1	abundance					1433:1441	the abundance	1429:1441	the abundance of Verrucomicrobia and E. coli (P < 0.05)	1429:1483	16S rRNA sequencing analyses revealed that E. coli challenge significantly increased the abundance of Verrucomicrobia and E. coli (P < 0.05).
32870484	3	74	theme	basal	729:733	arg1	Control					741:747	Control	741:747	Control	741:747	36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
32870484	3	74	theme	basal	729:733	arg1	diet					735:738	a basal diet	727:738	a basal diet (Control)	727:748	36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
32870484	8	75	theme	cecal	1690:1694	arg1	digesta					1696:1702	the cecal digesta	1686:1702	the cecal digesta	1686:1702	The concentrations of butyrate in the cecal digesta, which decreased due to the challenge, were higher in the E. coli + CS-Zn group (P < 0.05).
32870484	9	76	theme	cytokines	1889:1897	arg1	elevation					1859:1867	the elevation	1855:1867	the elevation of pro-inflammatory cytokines IL-6 concentration	1855:1916	In addition, CS-Zn supplementation significantly prevented the elevation of pro-inflammatory cytokines IL-6 concentration and up-regulated the level of anti-inflammatory cytokines IL-10 in cecal mucosa induced by E. coli infection (P < 0.05).
32870484	6	77	theme	sequencing	1353:1362	arg1	analyses					1364:1371	16S rRNA sequencing analyses	1344:1371	16S rRNA sequencing analyses	1344:1371	16S rRNA sequencing analyses revealed that E. coli challenge significantly increased the abundance of Verrucomicrobia and E. coli (P < 0.05).
32870484	5	78	dep	alleviated	1250:1259	arg1	<					1335:1335	P < 0.05	1333:1340	P < 0.05	1333:1340	CS-Zn supplementation mitigated intestinal morphology impairment (e.g. higher crypt depth and lower macroscopic damage index) induced by E. coli challenge (P < 0.05), and alleviated the increase of Myeloperoxidase (MPO) activity after E. coli challenge (P < 0.05).
32870484	9	79	dep	E.	2009:2010	arg1	coli					2012:2015	coli	2012:2015	coli	2012:2015	In addition, CS-Zn supplementation significantly prevented the elevation of pro-inflammatory cytokines IL-6 concentration and up-regulated the level of anti-inflammatory cytokines IL-10 in cecal mucosa induced by E. coli infection (P < 0.05).
32870484	5	80	theme	CS-Zn	1079:1083	arg1	supplementation					1085:1099	CS-Zn supplementation	1079:1099	CS-Zn supplementation	1079:1099	CS-Zn supplementation mitigated intestinal morphology impairment (e.g. higher crypt depth and lower macroscopic damage index) induced by E. coli challenge (P < 0.05), and alleviated the increase of Myeloperoxidase (MPO) activity after E. coli challenge (P < 0.05).
32870484	6	81	theme	16S	1344:1346	arg1	rRNA					1348:1351	16S rRNA	1344:1351	16S rRNA sequencing analyses	1344:1371	16S rRNA sequencing analyses revealed that E. coli challenge significantly increased the abundance of Verrucomicrobia and E. coli (P < 0.05).
32870484	1	82	theme	coli	148:151	arg1	infection					163:171	Escherichia coli (E. coli) infection	136:171	Escherichia coli (E. coli) infection	136:171	Escherichia coli (E. coli) infection is very common among young growing animals, and zinc supplementation is often used to alleviate inflammation induced by this disease.
32870484	9	83	theme	anti-inflammatory	1948:1964	arg1	cytokines					1966:1974	anti-inflammatory cytokines IL-10	1948:1980	anti-inflammatory cytokines IL-10	1948:1980	In addition, CS-Zn supplementation significantly prevented the elevation of pro-inflammatory cytokines IL-6 concentration and up-regulated the level of anti-inflammatory cytokines IL-10 in cecal mucosa induced by E. coli infection (P < 0.05).
32870484	4	84	theme	total	991:995	arg1	count					1006:1010	a total bacteria count	989:1010	a total bacteria count of 1010 CFU	989:1022	On days 10 to 12, each rat was given 4 ml of E. coli solution with a total bacteria count of 1010 CFU by oral gavage daily or normal saline of equal dosage.
32870484	10	85	theme	inflammatory	2283:2294	arg1	response					2296:2303	inflammatory response	2283:2303	inflammatory response	2283:2303	In conclusion, these results indicate that CS-Zn produces beneficial effects in alleviating gut mucosal injury of E. coli challenged rats by enhancing the intestinal morphology and modulating cecal bacterial composition, as well as attenuating inflammatory response.
32870484	5	86	theme	Myeloperoxidase	1277:1291	arg1	activity					1299:1306	Myeloperoxidase (MPO) activity	1277:1306	Myeloperoxidase (MPO) activity	1277:1306	CS-Zn supplementation mitigated intestinal morphology impairment (e.g. higher crypt depth and lower macroscopic damage index) induced by E. coli challenge (P < 0.05), and alleviated the increase of Myeloperoxidase (MPO) activity after E. coli challenge (P < 0.05).
32870484	9	87	theme	cecal	1985:1989	arg1	mucosa					1991:1996	cecal mucosa	1985:1996	cecal mucosa induced by E. coli infection (P < 0.05)	1985:2036	In addition, CS-Zn supplementation significantly prevented the elevation of pro-inflammatory cytokines IL-6 concentration and up-regulated the level of anti-inflammatory cytokines IL-10 in cecal mucosa induced by E. coli infection (P < 0.05).
32870484	4	88	dep	days	925:928	arg1	to					933:934	to	933:934	to	933:934	On days 10 to 12, each rat was given 4 ml of E. coli solution with a total bacteria count of 1010 CFU by oral gavage daily or normal saline of equal dosage.
32870484	2	89	theme	cecal	508:512	arg1	microbiota					514:523	cecal microbiota	508:523	cecal microbiota	508:523	Therefore, the objective of this study was to evaluate whether chitosan-chelated zinc (CS-Zn) supplementation could attenuate gut injury induced by E. coli challenge and to explore how CS-Zn modulates cecal microbiota and alleviates intestinal inflammation in weaned rats challenged with E. coli.
32870484	5	90	theme	higher	1150:1155	arg1	depth					1163:1167	higher crypt depth	1150:1167	higher crypt depth	1150:1167	CS-Zn supplementation mitigated intestinal morphology impairment (e.g. higher crypt depth and lower macroscopic damage index) induced by E. coli challenge (P < 0.05), and alleviated the increase of Myeloperoxidase (MPO) activity after E. coli challenge (P < 0.05).
32870484	8	91	theme	P	1785:1785	arg1	<					1787:1787	P < 0.05	1785:1792	P < 0.05	1785:1792	The concentrations of butyrate in the cecal digesta, which decreased due to the challenge, were higher in the E. coli + CS-Zn group (P < 0.05).
32870484	8	91	theme	P	1785:1785	arg1	group					1778:1782	the E. coli + CS-Zn group	1758:1782	the E. coli + CS-Zn group (P < 0.05)	1758:1793	The concentrations of butyrate in the cecal digesta, which decreased due to the challenge, were higher in the E. coli + CS-Zn group (P < 0.05).
32870484	2	92	theme	zinc	388:391	arg1	supplementation					401:415	chitosan-chelated zinc (CS-Zn) supplementation	370:415	chitosan-chelated zinc (CS-Zn) supplementation	370:415	Therefore, the objective of this study was to evaluate whether chitosan-chelated zinc (CS-Zn) supplementation could attenuate gut injury induced by E. coli challenge and to explore how CS-Zn modulates cecal microbiota and alleviates intestinal inflammation in weaned rats challenged with E. coli.
32870484	6	93	theme	E.	1387:1388	arg1	challenge					1395:1403	E. coli challenge	1387:1403	E. coli challenge	1387:1403	16S rRNA sequencing analyses revealed that E. coli challenge significantly increased the abundance of Verrucomicrobia and E. coli (P < 0.05).
32870484	5	94	dep	induced	1205:1211	arg1	<					1237:1237	P < 0.05	1235:1242	P < 0.05	1235:1242	CS-Zn supplementation mitigated intestinal morphology impairment (e.g. higher crypt depth and lower macroscopic damage index) induced by E. coli challenge (P < 0.05), and alleviated the increase of Myeloperoxidase (MPO) activity after E. coli challenge (P < 0.05).
32870484	3	95	theme	BW	638:639	arg1	g					633:633	55.65 ± 2.18 g	620:633	55.65 ± 2.18 g of BW	620:639	36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
32870484	5	96	theme	morphology	1122:1131	arg1	impairment					1133:1142	intestinal morphology impairment	1111:1142	intestinal morphology impairment (e.g. higher crypt depth and lower macroscopic damage index)	1111:1203	CS-Zn supplementation mitigated intestinal morphology impairment (e.g. higher crypt depth and lower macroscopic damage index) induced by E. coli challenge (P < 0.05), and alleviated the increase of Myeloperoxidase (MPO) activity after E. coli challenge (P < 0.05).
32870484	0	97	theme	cecal	33:37	arg1	microbiota					39:48	cecal microbiota	33:48	cecal microbiota	33:48	Chitosan-chelated zinc modulates cecal microbiota and attenuates inflammatory response in weaned rats challenged with Escherichia coli.
32870484	3	98	theme	mg/kg	843:847	arg1	CS-Zn					849:853	640 mg/kg CS-Zn	839:853	640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment	839:919	36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
32870484	10	99	theme	mucosal	2135:2141	arg1	injury					2143:2148	gut mucosal injury	2131:2148	gut mucosal injury of E. coli challenged rats	2131:2175	In conclusion, these results indicate that CS-Zn produces beneficial effects in alleviating gut mucosal injury of E. coli challenged rats by enhancing the intestinal morphology and modulating cecal bacterial composition, as well as attenuating inflammatory response.
32870484	3	100	theme	2.18	628:631	arg1	g					633:633	55.65 ± 2.18 g	620:633	55.65 ± 2.18 g of BW	620:639	36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
32870484	9	101	theme	P	2028:2028	arg1	infection					2017:2025	E. coli infection	2009:2025	E. coli infection (P < 0.05)	2009:2036	In addition, CS-Zn supplementation significantly prevented the elevation of pro-inflammatory cytokines IL-6 concentration and up-regulated the level of anti-inflammatory cytokines IL-10 in cecal mucosa induced by E. coli infection (P < 0.05).
32870484	9	101	theme	P	2028:2028	arg1	<					2030:2030	P < 0.05	2028:2035	P < 0.05	2028:2035	In addition, CS-Zn supplementation significantly prevented the elevation of pro-inflammatory cytokines IL-6 concentration and up-regulated the level of anti-inflammatory cytokines IL-10 in cecal mucosa induced by E. coli infection (P < 0.05).
32870484	2	102	theme	coli	458:461	arg1	challenge					463:471	E. coli challenge	455:471	E. coli challenge	455:471	Therefore, the objective of this study was to evaluate whether chitosan-chelated zinc (CS-Zn) supplementation could attenuate gut injury induced by E. coli challenge and to explore how CS-Zn modulates cecal microbiota and alleviates intestinal inflammation in weaned rats challenged with E. coli.
32870484	5	103	theme	P	1333:1333	arg1	<					1335:1335	P < 0.05	1333:1340	P < 0.05	1333:1340	CS-Zn supplementation mitigated intestinal morphology impairment (e.g. higher crypt depth and lower macroscopic damage index) induced by E. coli challenge (P < 0.05), and alleviated the increase of Myeloperoxidase (MPO) activity after E. coli challenge (P < 0.05).
32870484	3	104	theme	55.65	620:624	arg1	g					633:633	55.65 ± 2.18 g	620:633	55.65 ± 2.18 g of BW	620:639	36 weaned rats (55.65 ± 2.18 g of BW, n = 12) were divided into three treatment groups consisting of unchallenged rats fed a basal diet (Control) and two groups of rats challenged with E. coli and fed a basal diet or a diet containing 640 mg/kg CS-Zn (E. coli + CS-Zn, containing 50 mg/kg Zn) for a 14-day experiment.
32870484	2	105	theme	CS-Zn	394:398	arg1	supplementation					401:415	chitosan-chelated zinc (CS-Zn) supplementation	370:415	chitosan-chelated zinc (CS-Zn) supplementation	370:415	Therefore, the objective of this study was to evaluate whether chitosan-chelated zinc (CS-Zn) supplementation could attenuate gut injury induced by E. coli challenge and to explore how CS-Zn modulates cecal microbiota and alleviates intestinal inflammation in weaned rats challenged with E. coli.
32870484	0	106	theme	inflammatory	65:76	arg1	response					78:85	inflammatory response	65:85	inflammatory response in weaned rats challenged with Escherichia coli	65:133	Chitosan-chelated zinc modulates cecal microbiota and attenuates inflammatory response in weaned rats challenged with Escherichia coli.
32870484	7	107	theme	P	1641:1641	arg1	coli					1635:1638	E. coli	1632:1638	E. coli (P < 0.05)	1632:1649	However, CS-Zn supplementation increased the abundance of Lactobacillus and decreased the relative abundance of Proteobacteria, Desulfovibrio and E. coli (P < 0.05).
32870484	7	107	theme	P	1641:1641	arg1	<					1643:1643	P < 0.05	1641:1648	P < 0.05	1641:1648	However, CS-Zn supplementation increased the abundance of Lactobacillus and decreased the relative abundance of Proteobacteria, Desulfovibrio and E. coli (P < 0.05).
32870484	5	108	theme	activity	1299:1306	arg1	increase					1265:1272	the increase	1261:1272	the increase of Myeloperoxidase (MPO) activity	1261:1306	CS-Zn supplementation mitigated intestinal morphology impairment (e.g. higher crypt depth and lower macroscopic damage index) induced by E. coli challenge (P < 0.05), and alleviated the increase of Myeloperoxidase (MPO) activity after E. coli challenge (P < 0.05).
32870484	4	109	theme	equal	1065:1069	arg1	dosage					1071:1076	equal dosage	1065:1076	equal dosage	1065:1076	On days 10 to 12, each rat was given 4 ml of E. coli solution with a total bacteria count of 1010 CFU by oral gavage daily or normal saline of equal dosage.
32870484	7	110	theme	coli	1635:1638	arg1	abundance					1585:1593	the relative abundance	1572:1593	the relative abundance of Proteobacteria, Desulfovibrio and E. coli (P < 0.05)	1572:1649	However, CS-Zn supplementation increased the abundance of Lactobacillus and decreased the relative abundance of Proteobacteria, Desulfovibrio and E. coli (P < 0.05).
32870484	7	111	theme	Desulfovibrio	1614:1626	arg1	abundance					1585:1593	the relative abundance	1572:1593	the relative abundance of Proteobacteria, Desulfovibrio and E. coli (P < 0.05)	1572:1649	However, CS-Zn supplementation increased the abundance of Lactobacillus and decreased the relative abundance of Proteobacteria, Desulfovibrio and E. coli (P < 0.05).
32870484	5	112	theme	E.	1314:1315	arg1	challenge					1322:1330	E. coli challenge	1314:1330	E. coli challenge	1314:1330	CS-Zn supplementation mitigated intestinal morphology impairment (e.g. higher crypt depth and lower macroscopic damage index) induced by E. coli challenge (P < 0.05), and alleviated the increase of Myeloperoxidase (MPO) activity after E. coli challenge (P < 0.05).
32870484	5	113	theme	lower	1173:1177	arg1	index					1198:1202	lower macroscopic damage index	1173:1202	lower macroscopic damage index	1173:1202	CS-Zn supplementation mitigated intestinal morphology impairment (e.g. higher crypt depth and lower macroscopic damage index) induced by E. coli challenge (P < 0.05), and alleviated the increase of Myeloperoxidase (MPO) activity after E. coli challenge (P < 0.05).
32870484	1	114	theme	zinc	221:224	arg1	supplementation					226:240	zinc supplementation	221:240	zinc supplementation	221:240	Escherichia coli (E. coli) infection is very common among young growing animals, and zinc supplementation is often used to alleviate inflammation induced by this disease.
32870484	7	115	theme	Proteobacteria	1598:1611	arg1	abundance					1585:1593	the relative abundance	1572:1593	the relative abundance of Proteobacteria, Desulfovibrio and E. coli (P < 0.05)	1572:1649	However, CS-Zn supplementation increased the abundance of Lactobacillus and decreased the relative abundance of Proteobacteria, Desulfovibrio and E. coli (P < 0.05).
32870484	9	116	theme	E.	2009:2010	arg1	infection					2017:2025	E. coli infection	2009:2025	E. coli infection (P < 0.05)	2009:2036	In addition, CS-Zn supplementation significantly prevented the elevation of pro-inflammatory cytokines IL-6 concentration and up-regulated the level of anti-inflammatory cytokines IL-10 in cecal mucosa induced by E. coli infection (P < 0.05).
32870484	9	116	theme	E.	2009:2010	arg1	<					2030:2030	P < 0.05	2028:2035	P < 0.05	2028:2035	In addition, CS-Zn supplementation significantly prevented the elevation of pro-inflammatory cytokines IL-6 concentration and up-regulated the level of anti-inflammatory cytokines IL-10 in cecal mucosa induced by E. coli infection (P < 0.05).
32870484	2	117	from	inflammation	551:562	arg1	rats					574:577	weaned rats	567:577	weaned rats challenged with E. coli	567:601	Therefore, the objective of this study was to evaluate whether chitosan-chelated zinc (CS-Zn) supplementation could attenuate gut injury induced by E. coli challenge and to explore how CS-Zn modulates cecal microbiota and alleviates intestinal inflammation in weaned rats challenged with E. coli.
32870484	5	118	theme	damage	1191:1196	arg1	index					1198:1202	lower macroscopic damage index	1173:1202	lower macroscopic damage index	1173:1202	CS-Zn supplementation mitigated intestinal morphology impairment (e.g. higher crypt depth and lower macroscopic damage index) induced by E. coli challenge (P < 0.05), and alleviated the increase of Myeloperoxidase (MPO) activity after E. coli challenge (P < 0.05).
34318377	4	0	theme	optimum	861:867	arg1	conditions					869:878	the optimum conditions	857:878	the optimum conditions	857:878	Under the optimum conditions, the linearity was obtained in the range 1.5 to 700.0 ng mL-1 for morphine, codeine, oxycodone, and tramadol, and 0.5 to 1000.0 ng mL-1 for nalbuphine, thebaine, and noscapine with coefficient of determination (r2) ≥ 0.9990.
34318377	9	1	theme	PVA-PAA/CNT-CNC	1625:1639	arg1	nanofibers					1651:1660	PVA-PAA/CNT-CNC composite nanofibers	1625:1660	PVA-PAA/CNT-CNC composite nanofibers	1625:1660	B: Schematic presentation of applying PVA-PAA/CNT-CNC composite nanofibers as the sorbent in pipette-tip micro solid-phase extraction (PT-μSPE) for the preconcentration of seven opioid analgesic drugs in biological samples before HPLC-UV analysis.
34318377	9	1	theme	PVA-PAA/CNT-CNC	1625:1639	arg1	sorbent					1669:1675	the sorbent	1665:1675	the sorbent in pipette-tip micro solid-phase extraction (PT-μSPE) for the preconcentration of seven opioid analgesic drugs in biological samples before HPLC-UV analysis	1665:1832	B: Schematic presentation of applying PVA-PAA/CNT-CNC composite nanofibers as the sorbent in pipette-tip micro solid-phase extraction (PT-μSPE) for the preconcentration of seven opioid analgesic drugs in biological samples before HPLC-UV analysis.
34318377	0	2	theme	HPLC-UV	192:198	arg1	analysis					200:207	HPLC-UV analysis	192:207	HPLC-UV analysis	192:207	Simultaneous trace-level monitoring of seven opioid analgesic drugs in biological samples by pipette-tip micro solid phase extraction based on PVA-PAA/CNT-CNC composite nanofibers followed by HPLC-UV analysis.
34318377	1	3	theme	-poly	247:251	arg1	CNC					319:321	CNC	319:321	CNC	319:321	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	1	3	theme	-poly	247:251	arg1	nanocrystal					306:316	(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal	242:316	(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC)	242:322	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	2	4	theme	phase	585:589	arg1	extraction					591:600	pipette-tip micro-solid phase extraction	561:600	pipette-tip micro-solid phase extraction (PT-μSPE) of seven opioid analgesics (OAs)	561:643	The resultant composite was used as an effective and novel sorbent for pipette-tip micro-solid phase extraction (PT-μSPE) of seven opioid analgesics (OAs) in biological samples followed by HPLC-UV analysis.
34318377	2	4	theme	phase	585:589	arg1	PT-μSPE					603:609	PT-μSPE	603:609	PT-μSPE	603:609	The resultant composite was used as an effective and novel sorbent for pipette-tip micro-solid phase extraction (PT-μSPE) of seven opioid analgesics (OAs) in biological samples followed by HPLC-UV analysis.
34318377	1	5	theme	transform-infrared	409:426	arg1	spectroscopy					428:439	Fourier transform-infrared spectroscopy	401:439	Fourier transform-infrared spectroscopy	401:439	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	1	6	theme	acrylic	253:259	arg1	CNC					319:321	CNC	319:321	CNC	319:321	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	1	6	theme	acrylic	253:259	arg1	nanocrystal					306:316	(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal	242:316	(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC)	242:322	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	6	7	dep	%	1236:1236	arg1	n = 5					1250:1254	n = 5	1250:1254	n = 5	1250:1254	The relative standard deviations (RSDs) of 4.1-5.4% (intra-day, n = 5) and 5.2-6.4% (inter-day, n = 3) for three consecutive days were achieved.
34318377	6	7	dep	%	1236:1236	arg1	intra-day					1239:1247	intra-day	1239:1247	intra-day	1239:1247	The relative standard deviations (RSDs) of 4.1-5.4% (intra-day, n = 5) and 5.2-6.4% (inter-day, n = 3) for three consecutive days were achieved.
34318377	4	8	theme	r2	1091:1092	arg1	 ≥ 0.9990					1094:1102	determination (r2) ≥ 0.9990	1076:1102	determination (r2) ≥ 0.9990	1076:1102	Under the optimum conditions, the linearity was obtained in the range 1.5 to 700.0 ng mL-1 for morphine, codeine, oxycodone, and tramadol, and 0.5 to 1000.0 ng mL-1 for nalbuphine, thebaine, and noscapine with coefficient of determination (r2) ≥ 0.9990.
34318377	7	9	theme	PT-μSPE-HPLC-UV	1362:1376	arg1	method					1378:1383	the PT-μSPE-HPLC-UV method	1358:1383	the PT-μSPE-HPLC-UV method	1358:1383	Finally, the efficiency of the PT-μSPE-HPLC-UV method was evaluated for the determination of OAs in human plasma and urine samples with good recoveries (87.3 to 97.8%).
34318377	4	10	with	tramadol	980:987	arg1	coefficient					1061:1071	coefficient	1061:1071	coefficient of determination (r2) ≥ 0.9990	1061:1102	Under the optimum conditions, the linearity was obtained in the range 1.5 to 700.0 ng mL-1 for morphine, codeine, oxycodone, and tramadol, and 0.5 to 1000.0 ng mL-1 for nalbuphine, thebaine, and noscapine with coefficient of determination (r2) ≥ 0.9990.
34318377	4	11	theme	 ≥ 0.9990	1094:1102	arg1	coefficient					1061:1071	coefficient	1061:1071	coefficient of determination (r2) ≥ 0.9990	1061:1102	Under the optimum conditions, the linearity was obtained in the range 1.5 to 700.0 ng mL-1 for morphine, codeine, oxycodone, and tramadol, and 0.5 to 1000.0 ng mL-1 for nalbuphine, thebaine, and noscapine with coefficient of determination (r2) ≥ 0.9990.
34318377	3	12	theme	high	726:729	arg1	area					748:751	the high specific surface area	722:751	the high specific surface area	722:751	Addition of CNT-CNC with the high specific surface area and plenty of OH-functional groups endows the nanofibers with considerable extraction efficiency.
34318377	6	13	theme	relative	1190:1197	arg1	deviations					1208:1217	The relative standard deviations	1186:1217	The relative standard deviations (RSDs) of 4.1-5.4% (intra-day, n = 5) and 5.2-6.4% (inter-day, n = 3) for three consecutive	1186:1309	The relative standard deviations (RSDs) of 4.1-5.4% (intra-day, n = 5) and 5.2-6.4% (inter-day, n = 3) for three consecutive days were achieved.
34318377	6	13	theme	relative	1190:1197	arg1	RSDs					1220:1223	RSDs	1220:1223	RSDs	1220:1223	The relative standard deviations (RSDs) of 4.1-5.4% (intra-day, n = 5) and 5.2-6.4% (inter-day, n = 3) for three consecutive days were achieved.
34318377	1	14	theme	emission	451:458	arg1	microscopy					478:487	field emission scanning electron microscopy	445:487	field emission scanning electron microscopy	445:487	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	1	15	theme	Electrospun	210:220	arg1	PVA-PAA/CNT-CNC					325:339	PVA-PAA/CNT-CNC	325:339	PVA-PAA/CNT-CNC	325:339	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	1	15	theme	Electrospun	210:220	arg1	poly					222:225	Electrospun poly	210:225	Electrospun poly(vinyl alcohol)	210:240	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	1	15	theme	Electrospun	210:220	arg1	alcohol					233:239	vinyl alcohol	227:239	vinyl alcohol	227:239	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	9	16	theme	Schematic	1590:1598	arg1	presentation					1600:1611	Schematic presentation	1590:1611	B: Schematic presentation of applying PVA-PAA/CNT-CNC composite nanofibers as the sorbent in pipette-tip micro solid-phase extraction (PT-μSPE) for the preconcentration of seven opioid analgesic drugs in biological samples before HPLC-UV analysis.	1587:1833	B: Schematic presentation of applying PVA-PAA/CNT-CNC composite nanofibers as the sorbent in pipette-tip micro solid-phase extraction (PT-μSPE) for the preconcentration of seven opioid analgesic drugs in biological samples before HPLC-UV analysis.
34318377	0	17	from	samples	82:88	arg1	monitoring					25:34	Simultaneous trace-level monitoring	0:34	Simultaneous trace-level monitoring of seven opioid analgesic drugs in biological samples by pipette-tip micro solid phase extraction	0:132	Simultaneous trace-level monitoring of seven opioid analgesic drugs in biological samples by pipette-tip micro solid phase extraction based on PVA-PAA/CNT-CNC composite nanofibers followed by HPLC-UV analysis.
34318377	4	18	theme	determination	1076:1088	arg1	 ≥ 0.9990					1094:1102	determination (r2) ≥ 0.9990	1076:1102	determination (r2) ≥ 0.9990	1076:1102	Under the optimum conditions, the linearity was obtained in the range 1.5 to 700.0 ng mL-1 for morphine, codeine, oxycodone, and tramadol, and 0.5 to 1000.0 ng mL-1 for nalbuphine, thebaine, and noscapine with coefficient of determination (r2) ≥ 0.9990.
34318377	1	19	theme	composite	342:350	arg1	nanofibers					352:361	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers	210:361	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers	210:361	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	6	20	dep	%	1268:1268	arg1	n = 3					1282:1286	n = 3	1282:1286	n = 3	1282:1286	The relative standard deviations (RSDs) of 4.1-5.4% (intra-day, n = 5) and 5.2-6.4% (inter-day, n = 3) for three consecutive days were achieved.
34318377	6	20	dep	%	1268:1268	arg1	inter-day					1271:1279	inter-day	1271:1279	inter-day	1271:1279	The relative standard deviations (RSDs) of 4.1-5.4% (intra-day, n = 5) and 5.2-6.4% (inter-day, n = 3) for three consecutive days were achieved.
34318377	2	21	theme	pipette-tip	561:571	arg1	extraction					591:600	pipette-tip micro-solid phase extraction	561:600	pipette-tip micro-solid phase extraction (PT-μSPE) of seven opioid analgesics (OAs)	561:643	The resultant composite was used as an effective and novel sorbent for pipette-tip micro-solid phase extraction (PT-μSPE) of seven opioid analgesics (OAs) in biological samples followed by HPLC-UV analysis.
34318377	2	21	theme	pipette-tip	561:571	arg1	PT-μSPE					603:609	PT-μSPE	603:609	PT-μSPE	603:609	The resultant composite was used as an effective and novel sorbent for pipette-tip micro-solid phase extraction (PT-μSPE) of seven opioid analgesics (OAs) in biological samples followed by HPLC-UV analysis.
34318377	2	22	theme	HPLC-UV	679:685	arg1	analysis					687:694	HPLC-UV analysis	679:694	HPLC-UV analysis	679:694	The resultant composite was used as an effective and novel sorbent for pipette-tip micro-solid phase extraction (PT-μSPE) of seven opioid analgesics (OAs) in biological samples followed by HPLC-UV analysis.
34318377	3	23	theme	surface	740:746	arg1	area					748:751	the high specific surface area	722:751	the high specific surface area	722:751	Addition of CNT-CNC with the high specific surface area and plenty of OH-functional groups endows the nanofibers with considerable extraction efficiency.
34318377	0	24	theme	composite	159:167	arg1	nanofibers					169:178	PVA-PAA/CNT-CNC composite nanofibers	143:178	PVA-PAA/CNT-CNC composite nanofibers	143:178	Simultaneous trace-level monitoring of seven opioid analgesic drugs in biological samples by pipette-tip micro solid phase extraction based on PVA-PAA/CNT-CNC composite nanofibers followed by HPLC-UV analysis.
34318377	3	25	theme	groups	781:786	arg1	area					748:751	the high specific surface area	722:751	the high specific surface area	722:751	Addition of CNT-CNC with the high specific surface area and plenty of OH-functional groups endows the nanofibers with considerable extraction efficiency.
34318377	3	25	theme	groups	781:786	arg1	plenty					757:762	plenty	757:762	plenty	757:762	Addition of CNT-CNC with the high specific surface area and plenty of OH-functional groups endows the nanofibers with considerable extraction efficiency.
34318377	3	26	theme	extraction	828:837	arg1	efficiency					839:848	considerable extraction efficiency	815:848	considerable extraction efficiency	815:848	Addition of CNT-CNC with the high specific surface area and plenty of OH-functional groups endows the nanofibers with considerable extraction efficiency.
34318377	1	27	theme	CNTs	290:293	arg1	CNC					319:321	CNC	319:321	CNC	319:321	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	1	27	theme	CNTs	290:293	arg1	nanocrystal					306:316	(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal	242:316	(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC)	242:322	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	0	28	from	monitoring	25:34	arg1	samples					82:88	biological samples	71:88	biological samples	71:88	Simultaneous trace-level monitoring of seven opioid analgesic drugs in biological samples by pipette-tip micro solid phase extraction based on PVA-PAA/CNT-CNC composite nanofibers followed by HPLC-UV analysis.
34318377	1	29	theme	-cellulose	295:304	arg1	CNC					319:321	CNC	319:321	CNC	319:321	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	1	29	theme	-cellulose	295:304	arg1	nanocrystal					306:316	(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal	242:316	(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC)	242:322	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	9	30	theme	micro	1692:1696	arg1	PT-μSPE					1722:1728	PT-μSPE	1722:1728	PT-μSPE	1722:1728	B: Schematic presentation of applying PVA-PAA/CNT-CNC composite nanofibers as the sorbent in pipette-tip micro solid-phase extraction (PT-μSPE) for the preconcentration of seven opioid analgesic drugs in biological samples before HPLC-UV analysis.
34318377	9	30	theme	micro	1692:1696	arg1	extraction					1710:1719	pipette-tip micro solid-phase extraction	1680:1719	pipette-tip micro solid-phase extraction (PT-μSPE) for the preconcentration of seven opioid analgesic drugs in biological samples before HPLC-UV analysis	1680:1832	B: Schematic presentation of applying PVA-PAA/CNT-CNC composite nanofibers as the sorbent in pipette-tip micro solid-phase extraction (PT-μSPE) for the preconcentration of seven opioid analgesic drugs in biological samples before HPLC-UV analysis.
34318377	0	31	theme	pipette-tip	93:103	arg1	extraction					123:132	pipette-tip micro solid phase extraction	93:132	pipette-tip micro solid phase extraction	93:132	Simultaneous trace-level monitoring of seven opioid analgesic drugs in biological samples by pipette-tip micro solid phase extraction based on PVA-PAA/CNT-CNC composite nanofibers followed by HPLC-UV analysis.
34318377	7	32	theme	good	1467:1470	arg1	recoveries					1472:1481	good recoveries	1467:1481	good recoveries (87.3 to 97.8%)	1467:1497	Finally, the efficiency of the PT-μSPE-HPLC-UV method was evaluated for the determination of OAs in human plasma and urine samples with good recoveries (87.3 to 97.8%).
34318377	7	32	theme	good	1467:1470	arg1	%					1496:1496	87.3 to 97.8%	1484:1496	87.3 to 97.8%	1484:1496	Finally, the efficiency of the PT-μSPE-HPLC-UV method was evaluated for the determination of OAs in human plasma and urine samples with good recoveries (87.3 to 97.8%).
34318377	2	33	theme	opioid	621:626	arg1	OAs					640:642	OAs	640:642	OAs	640:642	The resultant composite was used as an effective and novel sorbent for pipette-tip micro-solid phase extraction (PT-μSPE) of seven opioid analgesics (OAs) in biological samples followed by HPLC-UV analysis.
34318377	2	33	theme	opioid	621:626	arg1	analgesics					628:637	seven opioid analgesics	615:637	seven opioid analgesics (OAs)	615:643	The resultant composite was used as an effective and novel sorbent for pipette-tip micro-solid phase extraction (PT-μSPE) of seven opioid analgesics (OAs) in biological samples followed by HPLC-UV analysis.
34318377	0	34	theme	solid	111:115	arg1	extraction					123:132	pipette-tip micro solid phase extraction	93:132	pipette-tip micro solid phase extraction	93:132	Simultaneous trace-level monitoring of seven opioid analgesic drugs in biological samples by pipette-tip micro solid phase extraction based on PVA-PAA/CNT-CNC composite nanofibers followed by HPLC-UV analysis.
34318377	9	35	dep	B	1587:1587	arg1	presentation					1600:1611	Schematic presentation	1590:1611	B: Schematic presentation of applying PVA-PAA/CNT-CNC composite nanofibers as the sorbent in pipette-tip micro solid-phase extraction (PT-μSPE) for the preconcentration of seven opioid analgesic drugs in biological samples before HPLC-UV analysis.	1587:1833	B: Schematic presentation of applying PVA-PAA/CNT-CNC composite nanofibers as the sorbent in pipette-tip micro solid-phase extraction (PT-μSPE) for the preconcentration of seven opioid analgesic drugs in biological samples before HPLC-UV analysis.
34318377	2	36	theme	biological	648:657	arg1	samples					659:665	biological samples	648:665	biological samples followed by HPLC-UV analysis	648:694	The resultant composite was used as an effective and novel sorbent for pipette-tip micro-solid phase extraction (PT-μSPE) of seven opioid analgesics (OAs) in biological samples followed by HPLC-UV analysis.
34318377	0	37	theme	Simultaneous	0:11	arg1	monitoring					25:34	Simultaneous trace-level monitoring	0:34	Simultaneous trace-level monitoring of seven opioid analgesic drugs in biological samples by pipette-tip micro solid phase extraction	0:132	Simultaneous trace-level monitoring of seven opioid analgesic drugs in biological samples by pipette-tip micro solid phase extraction based on PVA-PAA/CNT-CNC composite nanofibers followed by HPLC-UV analysis.
34318377	1	38	theme	acid	261:264	arg1	CNC					319:321	CNC	319:321	CNC	319:321	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	1	38	theme	acid	261:264	arg1	nanocrystal					306:316	(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal	242:316	(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC)	242:322	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	6	39	dep	days were	1311:1319	arg1	achieved					1321:1328	achieved	1321:1328	days were achieved	1311:1328	The relative standard deviations (RSDs) of 4.1-5.4% (intra-day, n = 5) and 5.2-6.4% (inter-day, n = 3) for three consecutive days were achieved.
34318377	7	40	theme	urine	1448:1452	arg1	samples					1454:1460	human plasma and urine samples	1431:1460	human plasma and urine samples with good recoveries (87.3 to 97.8%)	1431:1497	Finally, the efficiency of the PT-μSPE-HPLC-UV method was evaluated for the determination of OAs in human plasma and urine samples with good recoveries (87.3 to 97.8%).
34318377	5	41	theme	Detection	1105:1113	arg1	LODs					1123:1126	LODs	1123:1126	LODs	1123:1126	Detection limits (LODs) based on S/N = 3 were in the range of 0.15-0.50 ng mL-1.
34318377	5	41	theme	Detection	1105:1113	arg1	limits					1115:1120	Detection limits	1105:1120	Detection limits (LODs) based on S/N = 3	1105:1144	Detection limits (LODs) based on S/N = 3 were in the range of 0.15-0.50 ng mL-1.
34318377	7	42	theme	plasma	1437:1442	arg1	samples					1454:1460	human plasma and urine samples	1431:1460	human plasma and urine samples with good recoveries (87.3 to 97.8%)	1431:1497	Finally, the efficiency of the PT-μSPE-HPLC-UV method was evaluated for the determination of OAs in human plasma and urine samples with good recoveries (87.3 to 97.8%).
34318377	4	43	with	oxycodone	965:973	arg1	coefficient					1061:1071	coefficient	1061:1071	coefficient of determination (r2) ≥ 0.9990	1061:1102	Under the optimum conditions, the linearity was obtained in the range 1.5 to 700.0 ng mL-1 for morphine, codeine, oxycodone, and tramadol, and 0.5 to 1000.0 ng mL-1 for nalbuphine, thebaine, and noscapine with coefficient of determination (r2) ≥ 0.9990.
34318377	0	44	theme	biological	71:80	arg1	samples					82:88	biological samples	71:88	biological samples	71:88	Simultaneous trace-level monitoring of seven opioid analgesic drugs in biological samples by pipette-tip micro solid phase extraction based on PVA-PAA/CNT-CNC composite nanofibers followed by HPLC-UV analysis.
34318377	2	45	used	used	518:521	arg2	composite					504:512	The resultant composite	490:512	The resultant composite	490:512	The resultant composite was used as an effective and novel sorbent for pipette-tip micro-solid phase extraction (PT-μSPE) of seven opioid analgesics (OAs) in biological samples followed by HPLC-UV analysis.
34318377	2	45	used	used	518:521	arg2	sorbent					549:555	an effective and novel sorbent	526:555	an effective and novel sorbent for pipette-tip micro-solid phase extraction (PT-μSPE) of seven opioid analgesics (OAs)	526:643	The resultant composite was used as an effective and novel sorbent for pipette-tip micro-solid phase extraction (PT-μSPE) of seven opioid analgesics (OAs) in biological samples followed by HPLC-UV analysis.
34318377	0	46	theme	analgesic	52:60	arg1	drugs					62:66	seven opioid analgesic drugs	39:66	seven opioid analgesic drugs in biological samples	39:88	Simultaneous trace-level monitoring of seven opioid analgesic drugs in biological samples by pipette-tip micro solid phase extraction based on PVA-PAA/CNT-CNC composite nanofibers followed by HPLC-UV analysis.
34318377	1	47	theme	nanotubes	280:288	arg1	CNC					319:321	CNC	319:321	CNC	319:321	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	1	47	theme	nanotubes	280:288	arg1	nanocrystal					306:316	(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal	242:316	(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC)	242:322	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	4	48	with	codeine	956:962	arg1	coefficient					1061:1071	coefficient	1061:1071	coefficient of determination (r2) ≥ 0.9990	1061:1102	Under the optimum conditions, the linearity was obtained in the range 1.5 to 700.0 ng mL-1 for morphine, codeine, oxycodone, and tramadol, and 0.5 to 1000.0 ng mL-1 for nalbuphine, thebaine, and noscapine with coefficient of determination (r2) ≥ 0.9990.
34318377	9	49	theme	analgesic	1772:1780	arg1	drugs					1782:1786	seven opioid analgesic drugs	1759:1786	seven opioid analgesic drugs	1759:1786	B: Schematic presentation of applying PVA-PAA/CNT-CNC composite nanofibers as the sorbent in pipette-tip micro solid-phase extraction (PT-μSPE) for the preconcentration of seven opioid analgesic drugs in biological samples before HPLC-UV analysis.
34318377	3	50	with	Addition	697:704	arg1	area					748:751	the high specific surface area	722:751	the high specific surface area	722:751	Addition of CNT-CNC with the high specific surface area and plenty of OH-functional groups endows the nanofibers with considerable extraction efficiency.
34318377	3	50	with	Addition	697:704	arg1	plenty					757:762	plenty	757:762	plenty	757:762	Addition of CNT-CNC with the high specific surface area and plenty of OH-functional groups endows the nanofibers with considerable extraction efficiency.
34318377	4	51	with	morphine	946:953	arg1	coefficient					1061:1071	coefficient	1061:1071	coefficient of determination (r2) ≥ 0.9990	1061:1102	Under the optimum conditions, the linearity was obtained in the range 1.5 to 700.0 ng mL-1 for morphine, codeine, oxycodone, and tramadol, and 0.5 to 1000.0 ng mL-1 for nalbuphine, thebaine, and noscapine with coefficient of determination (r2) ≥ 0.9990.
34318377	8	52	theme	PVA-PAA/CNT-CNC	1549:1563	arg1	nanofibers					1575:1584	PVA-PAA/CNT-CNC composite nanofibers	1549:1584	PVA-PAA/CNT-CNC composite nanofibers	1549:1584	A: Schematic illustration for the preparation of PVA-PAA/CNT-CNC composite nanofibers.
34318377	3	53	theme	OH-functional	767:779	arg1	groups					781:786	OH-functional groups	767:786	OH-functional groups	767:786	Addition of CNT-CNC with the high specific surface area and plenty of OH-functional groups endows the nanofibers with considerable extraction efficiency.
34318377	8	54	theme	nanofibers	1575:1584	arg1	preparation					1534:1544	the preparation	1530:1544	the preparation of PVA-PAA/CNT-CNC composite nanofibers	1530:1584	A: Schematic illustration for the preparation of PVA-PAA/CNT-CNC composite nanofibers.
34318377	9	55	from	preconcentration	1739:1754	arg1	samples					1802:1808	biological samples	1791:1808	biological samples before HPLC-UV analysis	1791:1832	B: Schematic presentation of applying PVA-PAA/CNT-CNC composite nanofibers as the sorbent in pipette-tip micro solid-phase extraction (PT-μSPE) for the preconcentration of seven opioid analgesic drugs in biological samples before HPLC-UV analysis.
34318377	9	56	theme	HPLC-UV	1817:1823	arg1	analysis					1825:1832	HPLC-UV analysis	1817:1832	HPLC-UV analysis	1817:1832	B: Schematic presentation of applying PVA-PAA/CNT-CNC composite nanofibers as the sorbent in pipette-tip micro solid-phase extraction (PT-μSPE) for the preconcentration of seven opioid analgesic drugs in biological samples before HPLC-UV analysis.
34318377	8	57	theme	Schematic	1503:1511	arg1	illustration					1513:1524	Schematic illustration	1503:1524	A: Schematic illustration for the preparation of PVA-PAA/CNT-CNC composite nanofibers.	1500:1585	A: Schematic illustration for the preparation of PVA-PAA/CNT-CNC composite nanofibers.
34318377	1	58	theme	Fourier	401:407	arg1	spectroscopy					428:439	Fourier transform-infrared spectroscopy	401:439	Fourier transform-infrared spectroscopy	401:439	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	7	59	theme	method	1378:1383	arg1	efficiency					1344:1353	the efficiency	1340:1353	the efficiency of the PT-μSPE-HPLC-UV method	1340:1383	Finally, the efficiency of the PT-μSPE-HPLC-UV method was evaluated for the determination of OAs in human plasma and urine samples with good recoveries (87.3 to 97.8%).
34318377	2	60	theme	micro-solid	573:583	arg1	extraction					591:600	pipette-tip micro-solid phase extraction	561:600	pipette-tip micro-solid phase extraction (PT-μSPE) of seven opioid analgesics (OAs)	561:643	The resultant composite was used as an effective and novel sorbent for pipette-tip micro-solid phase extraction (PT-μSPE) of seven opioid analgesics (OAs) in biological samples followed by HPLC-UV analysis.
34318377	2	60	theme	micro-solid	573:583	arg1	PT-μSPE					603:609	PT-μSPE	603:609	PT-μSPE	603:609	The resultant composite was used as an effective and novel sorbent for pipette-tip micro-solid phase extraction (PT-μSPE) of seven opioid analgesics (OAs) in biological samples followed by HPLC-UV analysis.
34318377	3	61	theme	CNT-CNC	709:715	arg1	Addition					697:704	Addition	697:704	Addition of CNT-CNC with the high specific surface area and plenty of OH-functional groups	697:786	Addition of CNT-CNC with the high specific surface area and plenty of OH-functional groups endows the nanofibers with considerable extraction efficiency.
34318377	4	62	dep	1000.0 ng mL-1	1001:1014	arg1	to					998:999	to	998:999	to	998:999	Under the optimum conditions, the linearity was obtained in the range 1.5 to 700.0 ng mL-1 for morphine, codeine, oxycodone, and tramadol, and 0.5 to 1000.0 ng mL-1 for nalbuphine, thebaine, and noscapine with coefficient of determination (r2) ≥ 0.9990.
34318377	6	63	theme	standard	1199:1206	arg1	deviations					1208:1217	The relative standard deviations	1186:1217	The relative standard deviations (RSDs) of 4.1-5.4% (intra-day, n = 5) and 5.2-6.4% (inter-day, n = 3) for three consecutive	1186:1309	The relative standard deviations (RSDs) of 4.1-5.4% (intra-day, n = 5) and 5.2-6.4% (inter-day, n = 3) for three consecutive days were achieved.
34318377	6	63	theme	standard	1199:1206	arg1	RSDs					1220:1223	RSDs	1220:1223	RSDs	1220:1223	The relative standard deviations (RSDs) of 4.1-5.4% (intra-day, n = 5) and 5.2-6.4% (inter-day, n = 3) for three consecutive days were achieved.
34318377	1	64	theme	field	445:449	arg1	microscopy					478:487	field emission scanning electron microscopy	445:487	field emission scanning electron microscopy	445:487	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	3	65	theme	specific	731:738	arg1	area					748:751	the high specific surface area	722:751	the high specific surface area	722:751	Addition of CNT-CNC with the high specific surface area and plenty of OH-functional groups endows the nanofibers with considerable extraction efficiency.
34318377	1	66	theme	scanning	460:467	arg1	microscopy					478:487	field emission scanning electron microscopy	445:487	field emission scanning electron microscopy	445:487	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	1	67	theme	poly	222:225	arg1	nanofibers					352:361	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers	210:361	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers	210:361	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	2	68	theme	effective	529:537	arg1	sorbent					549:555	an effective and novel sorbent	526:555	an effective and novel sorbent for pipette-tip micro-solid phase extraction (PT-μSPE) of seven opioid analgesics (OAs)	526:643	The resultant composite was used as an effective and novel sorbent for pipette-tip micro-solid phase extraction (PT-μSPE) of seven opioid analgesics (OAs) in biological samples followed by HPLC-UV analysis.
34318377	2	68	theme	effective	529:537	arg1	composite					504:512	The resultant composite	490:512	The resultant composite	490:512	The resultant composite was used as an effective and novel sorbent for pipette-tip micro-solid phase extraction (PT-μSPE) of seven opioid analgesics (OAs) in biological samples followed by HPLC-UV analysis.
34318377	0	69	theme	PVA-PAA/CNT-CNC	143:157	arg1	nanofibers					169:178	PVA-PAA/CNT-CNC composite nanofibers	143:178	PVA-PAA/CNT-CNC composite nanofibers	143:178	Simultaneous trace-level monitoring of seven opioid analgesic drugs in biological samples by pipette-tip micro solid phase extraction based on PVA-PAA/CNT-CNC composite nanofibers followed by HPLC-UV analysis.
34318377	7	70	with	samples	1454:1460	arg1	recoveries					1472:1481	good recoveries	1467:1481	good recoveries (87.3 to 97.8%)	1467:1497	Finally, the efficiency of the PT-μSPE-HPLC-UV method was evaluated for the determination of OAs in human plasma and urine samples with good recoveries (87.3 to 97.8%).
34318377	7	70	with	samples	1454:1460	arg1	%					1496:1496	87.3 to 97.8%	1484:1496	87.3 to 97.8%	1484:1496	Finally, the efficiency of the PT-μSPE-HPLC-UV method was evaluated for the determination of OAs in human plasma and urine samples with good recoveries (87.3 to 97.8%).
34318377	1	71	theme	vinyl	227:231	arg1	poly					222:225	Electrospun poly	210:225	Electrospun poly(vinyl alcohol)	210:240	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	1	71	theme	vinyl	227:231	arg1	alcohol					233:239	vinyl alcohol	227:239	vinyl alcohol	227:239	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	3	72	with	nanofibers	799:808	arg1	efficiency					839:848	considerable extraction efficiency	815:848	considerable extraction efficiency	815:848	Addition of CNT-CNC with the high specific surface area and plenty of OH-functional groups endows the nanofibers with considerable extraction efficiency.
34318377	7	73	from	determination	1407:1419	arg1	samples					1454:1460	human plasma and urine samples	1431:1460	human plasma and urine samples with good recoveries (87.3 to 97.8%)	1431:1497	Finally, the efficiency of the PT-μSPE-HPLC-UV method was evaluated for the determination of OAs in human plasma and urine samples with good recoveries (87.3 to 97.8%).
34318377	9	74	theme	composite	1641:1649	arg1	nanofibers					1651:1660	PVA-PAA/CNT-CNC composite nanofibers	1625:1660	PVA-PAA/CNT-CNC composite nanofibers	1625:1660	B: Schematic presentation of applying PVA-PAA/CNT-CNC composite nanofibers as the sorbent in pipette-tip micro solid-phase extraction (PT-μSPE) for the preconcentration of seven opioid analgesic drugs in biological samples before HPLC-UV analysis.
34318377	9	74	theme	composite	1641:1649	arg1	sorbent					1669:1675	the sorbent	1665:1675	the sorbent in pipette-tip micro solid-phase extraction (PT-μSPE) for the preconcentration of seven opioid analgesic drugs in biological samples before HPLC-UV analysis	1665:1832	B: Schematic presentation of applying PVA-PAA/CNT-CNC composite nanofibers as the sorbent in pipette-tip micro solid-phase extraction (PT-μSPE) for the preconcentration of seven opioid analgesic drugs in biological samples before HPLC-UV analysis.
34318377	2	75	theme	novel	543:547	arg1	sorbent					549:555	an effective and novel sorbent	526:555	an effective and novel sorbent for pipette-tip micro-solid phase extraction (PT-μSPE) of seven opioid analgesics (OAs)	526:643	The resultant composite was used as an effective and novel sorbent for pipette-tip micro-solid phase extraction (PT-μSPE) of seven opioid analgesics (OAs) in biological samples followed by HPLC-UV analysis.
34318377	2	75	theme	novel	543:547	arg1	composite					504:512	The resultant composite	490:512	The resultant composite	490:512	The resultant composite was used as an effective and novel sorbent for pipette-tip micro-solid phase extraction (PT-μSPE) of seven opioid analgesics (OAs) in biological samples followed by HPLC-UV analysis.
34318377	6	76	theme	consecutive	1299:1309	arg1	deviations					1208:1217	The relative standard deviations	1186:1217	The relative standard deviations (RSDs) of 4.1-5.4% (intra-day, n = 5) and 5.2-6.4% (inter-day, n = 3) for three consecutive	1186:1309	The relative standard deviations (RSDs) of 4.1-5.4% (intra-day, n = 5) and 5.2-6.4% (inter-day, n = 3) for three consecutive days were achieved.
34318377	6	76	theme	consecutive	1299:1309	arg1	RSDs					1220:1223	RSDs	1220:1223	RSDs	1220:1223	The relative standard deviations (RSDs) of 4.1-5.4% (intra-day, n = 5) and 5.2-6.4% (inter-day, n = 3) for three consecutive days were achieved.
34318377	4	77	dep	range	915:919	arg1	to					925:926	to	925:926	to	925:926	Under the optimum conditions, the linearity was obtained in the range 1.5 to 700.0 ng mL-1 for morphine, codeine, oxycodone, and tramadol, and 0.5 to 1000.0 ng mL-1 for nalbuphine, thebaine, and noscapine with coefficient of determination (r2) ≥ 0.9990.
34318377	0	78	dep	based	134:138	arg1	followed					180:187	followed	180:187	followed by HPLC-UV analysis	180:207	Simultaneous trace-level monitoring of seven opioid analgesic drugs in biological samples by pipette-tip micro solid phase extraction based on PVA-PAA/CNT-CNC composite nanofibers followed by HPLC-UV analysis.
34318377	9	79	theme	pipette-tip	1680:1690	arg1	PT-μSPE					1722:1728	PT-μSPE	1722:1728	PT-μSPE	1722:1728	B: Schematic presentation of applying PVA-PAA/CNT-CNC composite nanofibers as the sorbent in pipette-tip micro solid-phase extraction (PT-μSPE) for the preconcentration of seven opioid analgesic drugs in biological samples before HPLC-UV analysis.
34318377	9	79	theme	pipette-tip	1680:1690	arg1	extraction					1710:1719	pipette-tip micro solid-phase extraction	1680:1719	pipette-tip micro solid-phase extraction (PT-μSPE) for the preconcentration of seven opioid analgesic drugs in biological samples before HPLC-UV analysis	1680:1832	B: Schematic presentation of applying PVA-PAA/CNT-CNC composite nanofibers as the sorbent in pipette-tip micro solid-phase extraction (PT-μSPE) for the preconcentration of seven opioid analgesic drugs in biological samples before HPLC-UV analysis.
34318377	3	80	theme	considerable	815:826	arg1	efficiency					839:848	considerable extraction efficiency	815:848	considerable extraction efficiency	815:848	Addition of CNT-CNC with the high specific surface area and plenty of OH-functional groups endows the nanofibers with considerable extraction efficiency.
34318377	0	81	theme	micro	105:109	arg1	extraction					123:132	pipette-tip micro solid phase extraction	93:132	pipette-tip micro solid phase extraction	93:132	Simultaneous trace-level monitoring of seven opioid analgesic drugs in biological samples by pipette-tip micro solid phase extraction based on PVA-PAA/CNT-CNC composite nanofibers followed by HPLC-UV analysis.
34318377	1	82	theme	nanocrystal	306:316	arg1	nanofibers					352:361	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers	210:361	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers	210:361	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	9	83	theme	solid-phase	1698:1708	arg1	PT-μSPE					1722:1728	PT-μSPE	1722:1728	PT-μSPE	1722:1728	B: Schematic presentation of applying PVA-PAA/CNT-CNC composite nanofibers as the sorbent in pipette-tip micro solid-phase extraction (PT-μSPE) for the preconcentration of seven opioid analgesic drugs in biological samples before HPLC-UV analysis.
34318377	9	83	theme	solid-phase	1698:1708	arg1	extraction					1710:1719	pipette-tip micro solid-phase extraction	1680:1719	pipette-tip micro solid-phase extraction (PT-μSPE) for the preconcentration of seven opioid analgesic drugs in biological samples before HPLC-UV analysis	1680:1832	B: Schematic presentation of applying PVA-PAA/CNT-CNC composite nanofibers as the sorbent in pipette-tip micro solid-phase extraction (PT-μSPE) for the preconcentration of seven opioid analgesic drugs in biological samples before HPLC-UV analysis.
34318377	0	84	from	drugs	62:66	arg1	samples					82:88	biological samples	71:88	biological samples	71:88	Simultaneous trace-level monitoring of seven opioid analgesic drugs in biological samples by pipette-tip micro solid phase extraction based on PVA-PAA/CNT-CNC composite nanofibers followed by HPLC-UV analysis.
34318377	2	85	theme	analgesics	628:637	arg1	extraction					591:600	pipette-tip micro-solid phase extraction	561:600	pipette-tip micro-solid phase extraction (PT-μSPE) of seven opioid analgesics (OAs)	561:643	The resultant composite was used as an effective and novel sorbent for pipette-tip micro-solid phase extraction (PT-μSPE) of seven opioid analgesics (OAs) in biological samples followed by HPLC-UV analysis.
34318377	2	85	theme	analgesics	628:637	arg1	PT-μSPE					603:609	PT-μSPE	603:609	PT-μSPE	603:609	The resultant composite was used as an effective and novel sorbent for pipette-tip micro-solid phase extraction (PT-μSPE) of seven opioid analgesics (OAs) in biological samples followed by HPLC-UV analysis.
34318377	0	86	theme	phase	117:121	arg1	extraction					123:132	pipette-tip micro solid phase extraction	93:132	pipette-tip micro solid phase extraction	93:132	Simultaneous trace-level monitoring of seven opioid analgesic drugs in biological samples by pipette-tip micro solid phase extraction based on PVA-PAA/CNT-CNC composite nanofibers followed by HPLC-UV analysis.
34318377	8	87	dep	A	1500:1500	arg1	illustration					1513:1524	Schematic illustration	1503:1524	A: Schematic illustration for the preparation of PVA-PAA/CNT-CNC composite nanofibers.	1500:1585	A: Schematic illustration for the preparation of PVA-PAA/CNT-CNC composite nanofibers.
34318377	2	88	theme	resultant	494:502	arg1	composite					504:512	The resultant composite	490:512	The resultant composite	490:512	The resultant composite was used as an effective and novel sorbent for pipette-tip micro-solid phase extraction (PT-μSPE) of seven opioid analgesics (OAs) in biological samples followed by HPLC-UV analysis.
34318377	2	88	theme	resultant	494:502	arg1	sorbent					549:555	an effective and novel sorbent	526:555	an effective and novel sorbent for pipette-tip micro-solid phase extraction (PT-μSPE) of seven opioid analgesics (OAs)	526:643	The resultant composite was used as an effective and novel sorbent for pipette-tip micro-solid phase extraction (PT-μSPE) of seven opioid analgesics (OAs) in biological samples followed by HPLC-UV analysis.
34318377	0	89	theme	trace-level	13:23	arg1	monitoring					25:34	Simultaneous trace-level monitoring	0:34	Simultaneous trace-level monitoring of seven opioid analgesic drugs in biological samples by pipette-tip micro solid phase extraction	0:132	Simultaneous trace-level monitoring of seven opioid analgesic drugs in biological samples by pipette-tip micro solid phase extraction based on PVA-PAA/CNT-CNC composite nanofibers followed by HPLC-UV analysis.
34318377	9	90	from	sorbent	1669:1675	arg1	PT-μSPE					1722:1728	PT-μSPE	1722:1728	PT-μSPE	1722:1728	B: Schematic presentation of applying PVA-PAA/CNT-CNC composite nanofibers as the sorbent in pipette-tip micro solid-phase extraction (PT-μSPE) for the preconcentration of seven opioid analgesic drugs in biological samples before HPLC-UV analysis.
34318377	9	90	from	sorbent	1669:1675	arg1	extraction					1710:1719	pipette-tip micro solid-phase extraction	1680:1719	pipette-tip micro solid-phase extraction (PT-μSPE) for the preconcentration of seven opioid analgesic drugs in biological samples before HPLC-UV analysis	1680:1832	B: Schematic presentation of applying PVA-PAA/CNT-CNC composite nanofibers as the sorbent in pipette-tip micro solid-phase extraction (PT-μSPE) for the preconcentration of seven opioid analgesic drugs in biological samples before HPLC-UV analysis.
34318377	0	91	theme	opioid	45:50	arg1	drugs					62:66	seven opioid analgesic drugs	39:66	seven opioid analgesic drugs in biological samples	39:88	Simultaneous trace-level monitoring of seven opioid analgesic drugs in biological samples by pipette-tip micro solid phase extraction based on PVA-PAA/CNT-CNC composite nanofibers followed by HPLC-UV analysis.
34318377	1	92	theme	PAA	268:270	arg1	CNC					319:321	CNC	319:321	CNC	319:321	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	1	92	theme	PAA	268:270	arg1	nanocrystal					306:316	(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal	242:316	(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC)	242:322	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	1	93	theme	electron	469:476	arg1	microscopy					478:487	field emission scanning electron microscopy	445:487	field emission scanning electron microscopy	445:487	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	0	94	theme	drugs	62:66	arg1	monitoring					25:34	Simultaneous trace-level monitoring	0:34	Simultaneous trace-level monitoring of seven opioid analgesic drugs in biological samples by pipette-tip micro solid phase extraction	0:132	Simultaneous trace-level monitoring of seven opioid analgesic drugs in biological samples by pipette-tip micro solid phase extraction based on PVA-PAA/CNT-CNC composite nanofibers followed by HPLC-UV analysis.
34318377	1	95	theme	/carbon	272:278	arg1	CNC					319:321	CNC	319:321	CNC	319:321	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	1	95	theme	/carbon	272:278	arg1	nanocrystal					306:316	(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal	242:316	(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC)	242:322	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	9	96	theme	opioid	1765:1770	arg1	drugs					1782:1786	seven opioid analgesic drugs	1759:1786	seven opioid analgesic drugs	1759:1786	B: Schematic presentation of applying PVA-PAA/CNT-CNC composite nanofibers as the sorbent in pipette-tip micro solid-phase extraction (PT-μSPE) for the preconcentration of seven opioid analgesic drugs in biological samples before HPLC-UV analysis.
34318377	7	97	dep	97.8	1492:1495	arg1	to					1489:1490	to	1489:1490	to	1489:1490	Finally, the efficiency of the PT-μSPE-HPLC-UV method was evaluated for the determination of OAs in human plasma and urine samples with good recoveries (87.3 to 97.8%).
34318377	7	98	theme	human	1431:1435	arg1	samples					1454:1460	human plasma and urine samples	1431:1460	human plasma and urine samples with good recoveries (87.3 to 97.8%)	1431:1497	Finally, the efficiency of the PT-μSPE-HPLC-UV method was evaluated for the determination of OAs in human plasma and urine samples with good recoveries (87.3 to 97.8%).
34318377	9	99	theme	drugs	1782:1786	arg1	preconcentration					1739:1754	the preconcentration	1735:1754	the preconcentration of seven opioid analgesic drugs in biological samples before HPLC-UV analysis	1735:1832	B: Schematic presentation of applying PVA-PAA/CNT-CNC composite nanofibers as the sorbent in pipette-tip micro solid-phase extraction (PT-μSPE) for the preconcentration of seven opioid analgesic drugs in biological samples before HPLC-UV analysis.
34318377	8	100	theme	composite	1565:1573	arg1	nanofibers					1575:1584	PVA-PAA/CNT-CNC composite nanofibers	1549:1584	PVA-PAA/CNT-CNC composite nanofibers	1549:1584	A: Schematic illustration for the preparation of PVA-PAA/CNT-CNC composite nanofibers.
34318377	5	101	theme	0.15-0.50 ng mL-1	1167:1183	arg1	range					1158:1162	the range	1154:1162	the range of 0.15-0.50 ng mL-1	1154:1183	Detection limits (LODs) based on S/N = 3 were in the range of 0.15-0.50 ng mL-1.
34318377	7	102	theme	OAs	1424:1426	arg1	determination					1407:1419	the determination	1403:1419	the determination of OAs in human plasma and urine samples with good recoveries (87.3 to 97.8%)	1403:1497	Finally, the efficiency of the PT-μSPE-HPLC-UV method was evaluated for the determination of OAs in human plasma and urine samples with good recoveries (87.3 to 97.8%).
34318377	9	103	theme	biological	1791:1800	arg1	samples					1802:1808	biological samples	1791:1808	biological samples before HPLC-UV analysis	1791:1832	B: Schematic presentation of applying PVA-PAA/CNT-CNC composite nanofibers as the sorbent in pipette-tip micro solid-phase extraction (PT-μSPE) for the preconcentration of seven opioid analgesic drugs in biological samples before HPLC-UV analysis.
34318377	1	104	theme	PVA	243:245	arg1	CNC					319:321	CNC	319:321	CNC	319:321	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34318377	1	104	theme	PVA	243:245	arg1	nanocrystal					306:316	(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal	242:316	(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC)	242:322	Electrospun poly(vinyl alcohol)-(PVA)-poly(acrylic acid) (PAA)/carbon nanotubes(CNTs)-cellulose nanocrystal (CNC) (PVA-PAA/CNT-CNC) composite nanofibers were prepared and characterized using Fourier transform-infrared spectroscopy and field emission scanning electron microscopy.
34918475	1	0	theme	tissue	424:429	arg1	characteristics					388:402	mechanical characteristics	377:402	mechanical characteristics	377:402	Although tissue engineering has attracted increasing attention for the treatment of degenerative intervertebral disc disease, the biochemical properties, structural organization, and mechanical characteristics of annulus fibrosus tissue have restricted progress.
34918475	1	0	theme	tissue	424:429	arg1	properties					336:345	the biochemical properties	320:345	the biochemical properties	320:345	Although tissue engineering has attracted increasing attention for the treatment of degenerative intervertebral disc disease, the biochemical properties, structural organization, and mechanical characteristics of annulus fibrosus tissue have restricted progress.
34918475	1	0	theme	tissue	424:429	arg1	organization					359:370	structural organization	348:370	structural organization	348:370	Although tissue engineering has attracted increasing attention for the treatment of degenerative intervertebral disc disease, the biochemical properties, structural organization, and mechanical characteristics of annulus fibrosus tissue have restricted progress.
34918475	6	1	theme	aggrecan	1369:1376	arg1	contents					1378:1385	collagen type II and aggrecan contents	1348:1385	collagen type II and aggrecan contents	1348:1385	Regarding extracellular matrix secretion, collagen type I content gradually increased with substrate elasticity, while collagen type II and aggrecan contents decreased.
34918475	1	2	theme	degenerative	278:289	arg1	disease					311:317	degenerative intervertebral disc disease	278:317	degenerative intervertebral disc disease	278:317	Although tissue engineering has attracted increasing attention for the treatment of degenerative intervertebral disc disease, the biochemical properties, structural organization, and mechanical characteristics of annulus fibrosus tissue have restricted progress.
34918475	7	3	theme	tissue	1618:1623	arg1	defects					1590:1596	defects	1590:1596	defects of annulus fibrosus tissue	1590:1623	In vivo evaluations employing magnetic resonance imaging, hematoxylin and eosin staining, and immunohistochemistry indicated that DAFM/PECUU-blended fibrous scaffolds could effectively repair defects of annulus fibrosus tissue.
34918475	4	4	theme	native	936:941	arg1	tissue					976:981	native inner and outer annulus fibrosus tissue	936:981	native inner and outer annulus fibrosus tissue	936:981	In this study, we used a coaxial electrospinning method to synthesize DAFM/PECUU-blended fibrous scaffolds with elasticities approximating that of native inner and outer annulus fibrosus tissue.
34918475	6	5	theme	collagen	1271:1278	arg1	type					1280:1283	collagen type I	1271:1285	collagen type I content	1271:1293	Regarding extracellular matrix secretion, collagen type I content gradually increased with substrate elasticity, while collagen type II and aggrecan contents decreased.
34918475	1	6	theme	disc	306:309	arg1	disease					311:317	degenerative intervertebral disc disease	278:317	degenerative intervertebral disc disease	278:317	Although tissue engineering has attracted increasing attention for the treatment of degenerative intervertebral disc disease, the biochemical properties, structural organization, and mechanical characteristics of annulus fibrosus tissue have restricted progress.
34918475	7	7	theme	annulus	1601:1607	arg1	tissue					1618:1623	annulus fibrosus tissue	1601:1623	annulus fibrosus tissue	1601:1623	In vivo evaluations employing magnetic resonance imaging, hematoxylin and eosin staining, and immunohistochemistry indicated that DAFM/PECUU-blended fibrous scaffolds could effectively repair defects of annulus fibrosus tissue.
34918475	7	8	theme	fibrous	1547:1553	arg1	scaffolds					1555:1563	DAFM/PECUU-blended fibrous scaffolds	1528:1563	DAFM/PECUU-blended fibrous scaffolds	1528:1563	In vivo evaluations employing magnetic resonance imaging, hematoxylin and eosin staining, and immunohistochemistry indicated that DAFM/PECUU-blended fibrous scaffolds could effectively repair defects of annulus fibrosus tissue.
34918475	6	9	theme	collagen	1348:1355	arg1	type					1357:1360	collagen type II	1348:1363	collagen type II	1348:1363	Regarding extracellular matrix secretion, collagen type I content gradually increased with substrate elasticity, while collagen type II and aggrecan contents decreased.
34918475	6	10	theme	extracellular	1239:1251	arg1	secretion					1260:1268	extracellular matrix secretion	1239:1268	extracellular matrix secretion	1239:1268	Regarding extracellular matrix secretion, collagen type I content gradually increased with substrate elasticity, while collagen type II and aggrecan contents decreased.
34918475	1	11	theme	tissue	203:208	arg1	engineering					210:220	tissue engineering	203:220	tissue engineering	203:220	Although tissue engineering has attracted increasing attention for the treatment of degenerative intervertebral disc disease, the biochemical properties, structural organization, and mechanical characteristics of annulus fibrosus tissue have restricted progress.
34918475	3	12	contain	has	667:669	arg1	matrix					653:658	Decellularized annulus fibrosus matrix	621:658	Decellularized annulus fibrosus matrix (DAFM)	621:665	Decellularized annulus fibrosus matrix (DAFM) has good biocompatibility and biodegradability, making it suitable for cell adhesion, proliferation, and differentiation.
34918475	3	12	contain	has	667:669	arg2	biodegradability					697:712	biodegradability	697:712	biodegradability	697:712	Decellularized annulus fibrosus matrix (DAFM) has good biocompatibility and biodegradability, making it suitable for cell adhesion, proliferation, and differentiation.
34918475	3	12	contain	has	667:669	arg2	biocompatibility					676:691	good biocompatibility	671:691	good biocompatibility	671:691	Decellularized annulus fibrosus matrix (DAFM) has good biocompatibility and biodegradability, making it suitable for cell adhesion, proliferation, and differentiation.
34918475	3	12	contain	has	667:669	arg1	DAFM					661:664	DAFM	661:664	DAFM	661:664	Decellularized annulus fibrosus matrix (DAFM) has good biocompatibility and biodegradability, making it suitable for cell adhesion, proliferation, and differentiation.
34918475	4	13	used	used	807:810	arg2	we					804:805	we	804:805	we	804:805	In this study, we used a coaxial electrospinning method to synthesize DAFM/PECUU-blended fibrous scaffolds with elasticities approximating that of native inner and outer annulus fibrosus tissue.
34918475	2	14	theme	ether	582:586	arg1	urethane					598:605	ether carbonate urethane	582:605	ether carbonate urethane	582:605	Differentiation of annulus fibrosus-derived stem cells (AFSCs) can be regulated by the elasticity of substrates such as poly(ether carbonate urethane)urea (PECUU).
34918475	2	14	theme	ether	582:586	arg1	poly					577:580	poly	577:580	poly(ether carbonate urethane)urea (PECUU)	577:618	Differentiation of annulus fibrosus-derived stem cells (AFSCs) can be regulated by the elasticity of substrates such as poly(ether carbonate urethane)urea (PECUU).
34918475	5	15	theme	collagen	1153:1160	arg1	type					1162:1165	collagen type II	1153:1168	collagen type II	1153:1168	AFSCs cultured on DAFM/PECUU-blended fibrous scaffolds exhibited increased collagen type I gene expression with increasing elasticity of the scaffold material; notably, collagen type II and aggrecan gene expression exhibited the opposite trend.
34918475	3	16	theme	annulus	636:642	arg1	matrix					653:658	Decellularized annulus fibrosus matrix	621:658	Decellularized annulus fibrosus matrix (DAFM)	621:665	Decellularized annulus fibrosus matrix (DAFM) has good biocompatibility and biodegradability, making it suitable for cell adhesion, proliferation, and differentiation.
34918475	3	16	theme	annulus	636:642	arg1	DAFM					661:664	DAFM	661:664	DAFM	661:664	Decellularized annulus fibrosus matrix (DAFM) has good biocompatibility and biodegradability, making it suitable for cell adhesion, proliferation, and differentiation.
34918475	1	17	theme	increasing	236:245	arg1	attention					247:255	increasing attention	236:255	increasing attention	236:255	Although tissue engineering has attracted increasing attention for the treatment of degenerative intervertebral disc disease, the biochemical properties, structural organization, and mechanical characteristics of annulus fibrosus tissue have restricted progress.
34918475	7	18	theme	eosin	1472:1476	arg1	staining					1478:1485	hematoxylin and eosin staining	1456:1485	hematoxylin and eosin staining	1456:1485	In vivo evaluations employing magnetic resonance imaging, hematoxylin and eosin staining, and immunohistochemistry indicated that DAFM/PECUU-blended fibrous scaffolds could effectively repair defects of annulus fibrosus tissue.
34918475	4	19	theme	annulus	959:965	arg1	tissue					976:981	native inner and outer annulus fibrosus tissue	936:981	native inner and outer annulus fibrosus tissue	936:981	In this study, we used a coaxial electrospinning method to synthesize DAFM/PECUU-blended fibrous scaffolds with elasticities approximating that of native inner and outer annulus fibrosus tissue.
34918475	7	20	theme	hematoxylin	1456:1466	arg1	staining					1478:1485	hematoxylin and eosin staining	1456:1485	hematoxylin and eosin staining	1456:1485	In vivo evaluations employing magnetic resonance imaging, hematoxylin and eosin staining, and immunohistochemistry indicated that DAFM/PECUU-blended fibrous scaffolds could effectively repair defects of annulus fibrosus tissue.
34918475	0	21	theme	varying	170:176	arg1	moduli					186:191	varying elastic moduli	170:191	varying elastic moduli	170:191	The experimental study of regeneration of annulus fibrosus using decellularized annulus fibrosus matrix/poly(ether carbonate urethane)urea-blended fibrous scaffolds with varying elastic moduli.
34918475	5	22	theme	material	1134:1141	arg1	elasticity					1107:1116	elasticity	1107:1116	elasticity of the scaffold material	1107:1141	AFSCs cultured on DAFM/PECUU-blended fibrous scaffolds exhibited increased collagen type I gene expression with increasing elasticity of the scaffold material; notably, collagen type II and aggrecan gene expression exhibited the opposite trend.
34918475	0	23	theme	annulus	80:86	arg1	urethane					125:132	ether carbonate urethane	109:132	ether carbonate urethane	109:132	The experimental study of regeneration of annulus fibrosus using decellularized annulus fibrosus matrix/poly(ether carbonate urethane)urea-blended fibrous scaffolds with varying elastic moduli.
34918475	0	23	theme	annulus	80:86	arg1	matrix/poly					97:107	decellularized annulus fibrosus matrix/poly	65:107	decellularized annulus fibrosus matrix/poly(ether carbonate urethane)	65:133	The experimental study of regeneration of annulus fibrosus using decellularized annulus fibrosus matrix/poly(ether carbonate urethane)urea-blended fibrous scaffolds with varying elastic moduli.
34918475	1	24	theme	mechanical	377:386	arg1	characteristics					388:402	mechanical characteristics	377:402	mechanical characteristics	377:402	Although tissue engineering has attracted increasing attention for the treatment of degenerative intervertebral disc disease, the biochemical properties, structural organization, and mechanical characteristics of annulus fibrosus tissue have restricted progress.
34918475	4	25	with	scaffolds	886:894	arg1	elasticities					901:912	elasticities	901:912	elasticities approximating that of native inner and outer annulus fibrosus tissue	901:981	In this study, we used a coaxial electrospinning method to synthesize DAFM/PECUU-blended fibrous scaffolds with elasticities approximating that of native inner and outer annulus fibrosus tissue.
34918475	2	26	theme	poly	577:580	arg1	urea					607:610	poly(ether carbonate urethane)urea	577:610	poly(ether carbonate urethane)urea (PECUU)	577:618	Differentiation of annulus fibrosus-derived stem cells (AFSCs) can be regulated by the elasticity of substrates such as poly(ether carbonate urethane)urea (PECUU).
34918475	2	26	theme	poly	577:580	arg1	PECUU					613:617	PECUU	613:617	PECUU	613:617	Differentiation of annulus fibrosus-derived stem cells (AFSCs) can be regulated by the elasticity of substrates such as poly(ether carbonate urethane)urea (PECUU).
34918475	0	27	with	scaffolds	155:163	arg1	moduli					186:191	varying elastic moduli	170:191	varying elastic moduli	170:191	The experimental study of regeneration of annulus fibrosus using decellularized annulus fibrosus matrix/poly(ether carbonate urethane)urea-blended fibrous scaffolds with varying elastic moduli.
34918475	0	28	theme	ether	109:113	arg1	urethane					125:132	ether carbonate urethane	109:132	ether carbonate urethane	109:132	The experimental study of regeneration of annulus fibrosus using decellularized annulus fibrosus matrix/poly(ether carbonate urethane)urea-blended fibrous scaffolds with varying elastic moduli.
34918475	0	28	theme	ether	109:113	arg1	matrix/poly					97:107	decellularized annulus fibrosus matrix/poly	65:107	decellularized annulus fibrosus matrix/poly(ether carbonate urethane)	65:133	The experimental study of regeneration of annulus fibrosus using decellularized annulus fibrosus matrix/poly(ether carbonate urethane)urea-blended fibrous scaffolds with varying elastic moduli.
34918475	1	29	theme	fibrosus	415:422	arg1	tissue					424:429	annulus fibrosus tissue	407:429	annulus fibrosus tissue	407:429	Although tissue engineering has attracted increasing attention for the treatment of degenerative intervertebral disc disease, the biochemical properties, structural organization, and mechanical characteristics of annulus fibrosus tissue have restricted progress.
34918475	5	30	theme	collagen	1059:1066	arg1	type					1068:1071	collagen type I	1059:1073	increased collagen type I gene expression	1049:1089	AFSCs cultured on DAFM/PECUU-blended fibrous scaffolds exhibited increased collagen type I gene expression with increasing elasticity of the scaffold material; notably, collagen type II and aggrecan gene expression exhibited the opposite trend.
34918475	7	31	theme	resonance	1437:1445	arg1	imaging					1447:1453	magnetic resonance imaging	1428:1453	magnetic resonance imaging	1428:1453	In vivo evaluations employing magnetic resonance imaging, hematoxylin and eosin staining, and immunohistochemistry indicated that DAFM/PECUU-blended fibrous scaffolds could effectively repair defects of annulus fibrosus tissue.
34918475	1	32	theme	disease	311:317	arg1	treatment					265:273	the treatment	261:273	the treatment of degenerative intervertebral disc disease	261:317	Although tissue engineering has attracted increasing attention for the treatment of degenerative intervertebral disc disease, the biochemical properties, structural organization, and mechanical characteristics of annulus fibrosus tissue have restricted progress.
34918475	2	33	theme	cells	506:510	arg1	Differentiation					457:471	Differentiation	457:471	Differentiation of annulus fibrosus-derived stem cells (AFSCs)	457:518	Differentiation of annulus fibrosus-derived stem cells (AFSCs) can be regulated by the elasticity of substrates such as poly(ether carbonate urethane)urea (PECUU).
34918475	2	34	theme	fibrosus-derived	484:499	arg1	AFSCs					513:517	AFSCs	513:517	AFSCs	513:517	Differentiation of annulus fibrosus-derived stem cells (AFSCs) can be regulated by the elasticity of substrates such as poly(ether carbonate urethane)urea (PECUU).
34918475	2	34	theme	fibrosus-derived	484:499	arg1	cells					506:510	annulus fibrosus-derived stem cells	476:510	annulus fibrosus-derived stem cells (AFSCs)	476:518	Differentiation of annulus fibrosus-derived stem cells (AFSCs) can be regulated by the elasticity of substrates such as poly(ether carbonate urethane)urea (PECUU).
34918475	5	35	theme	fibrous	1021:1027	arg1	scaffolds					1029:1037	DAFM/PECUU-blended fibrous scaffolds	1002:1037	DAFM/PECUU-blended fibrous scaffolds	1002:1037	AFSCs cultured on DAFM/PECUU-blended fibrous scaffolds exhibited increased collagen type I gene expression with increasing elasticity of the scaffold material; notably, collagen type II and aggrecan gene expression exhibited the opposite trend.
34918475	0	36	theme	regeneration	26:37	arg1	study					17:21	The experimental study	0:21	The experimental study of regeneration of annulus fibrosus using decellularized annulus fibrosus matrix/poly(ether carbonate urethane)	0:133	The experimental study of regeneration of annulus fibrosus using decellularized annulus fibrosus matrix/poly(ether carbonate urethane)urea-blended fibrous scaffolds with varying elastic moduli.
34918475	8	37	theme	annulus	1711:1717	arg1	tissue					1728:1733	bionic annulus fibrosus tissue	1704:1733	bionic annulus fibrosus tissue that closely mimics the biological properties, mechanical function, and matrix composition of native tissue	1704:1841	Our findings provide a theoretical and practical basis for the development of bionic annulus fibrosus tissue that closely mimics the biological properties, mechanical function, and matrix composition of native tissue.
34918475	4	38	theme	DAFM/PECUU-blended	859:876	arg1	scaffolds					886:894	DAFM/PECUU-blended fibrous scaffolds	859:894	DAFM/PECUU-blended fibrous scaffolds with elasticities approximating that of native inner and outer annulus fibrosus tissue	859:981	In this study, we used a coaxial electrospinning method to synthesize DAFM/PECUU-blended fibrous scaffolds with elasticities approximating that of native inner and outer annulus fibrosus tissue.
34918475	0	39	theme	annulus	42:48	arg1	fibrosus					50:57	annulus fibrosus	42:57	annulus fibrosus using decellularized annulus fibrosus matrix/poly(ether carbonate urethane)	42:133	The experimental study of regeneration of annulus fibrosus using decellularized annulus fibrosus matrix/poly(ether carbonate urethane)urea-blended fibrous scaffolds with varying elastic moduli.
34918475	1	40	theme	structural	348:357	arg1	organization					359:370	structural organization	348:370	structural organization	348:370	Although tissue engineering has attracted increasing attention for the treatment of degenerative intervertebral disc disease, the biochemical properties, structural organization, and mechanical characteristics of annulus fibrosus tissue have restricted progress.
34918475	8	41	theme	tissue	1728:1733	arg1	development					1689:1699	the development	1685:1699	the development of bionic annulus fibrosus tissue that closely mimics the biological properties, mechanical function, and matrix composition of native tissue	1685:1841	Our findings provide a theoretical and practical basis for the development of bionic annulus fibrosus tissue that closely mimics the biological properties, mechanical function, and matrix composition of native tissue.
34918475	4	42	theme	electrospinning	822:836	arg1	method					838:843	a coaxial electrospinning method	812:843	a coaxial electrospinning method	812:843	In this study, we used a coaxial electrospinning method to synthesize DAFM/PECUU-blended fibrous scaffolds with elasticities approximating that of native inner and outer annulus fibrosus tissue.
34918475	8	43	theme	matrix	1807:1812	arg1	composition					1814:1824	matrix composition	1807:1824	matrix composition	1807:1824	Our findings provide a theoretical and practical basis for the development of bionic annulus fibrosus tissue that closely mimics the biological properties, mechanical function, and matrix composition of native tissue.
34918475	3	44	theme	cell	738:741	arg1	adhesion					743:750	cell adhesion	738:750	cell adhesion	738:750	Decellularized annulus fibrosus matrix (DAFM) has good biocompatibility and biodegradability, making it suitable for cell adhesion, proliferation, and differentiation.
34918475	7	45	theme	fibrosus	1609:1616	arg1	tissue					1618:1623	annulus fibrosus tissue	1601:1623	annulus fibrosus tissue	1601:1623	In vivo evaluations employing magnetic resonance imaging, hematoxylin and eosin staining, and immunohistochemistry indicated that DAFM/PECUU-blended fibrous scaffolds could effectively repair defects of annulus fibrosus tissue.
34918475	8	46	theme	tissue	1836:1841	arg1	composition					1814:1824	matrix composition	1807:1824	matrix composition	1807:1824	Our findings provide a theoretical and practical basis for the development of bionic annulus fibrosus tissue that closely mimics the biological properties, mechanical function, and matrix composition of native tissue.
34918475	8	46	theme	tissue	1836:1841	arg1	properties					1770:1779	the biological properties	1755:1779	the biological properties	1755:1779	Our findings provide a theoretical and practical basis for the development of bionic annulus fibrosus tissue that closely mimics the biological properties, mechanical function, and matrix composition of native tissue.
34918475	8	46	theme	tissue	1836:1841	arg1	function					1793:1800	mechanical function	1782:1800	mechanical function	1782:1800	Our findings provide a theoretical and practical basis for the development of bionic annulus fibrosus tissue that closely mimics the biological properties, mechanical function, and matrix composition of native tissue.
34918475	1	47	theme	intervertebral	291:304	arg1	disease					311:317	degenerative intervertebral disc disease	278:317	degenerative intervertebral disc disease	278:317	Although tissue engineering has attracted increasing attention for the treatment of degenerative intervertebral disc disease, the biochemical properties, structural organization, and mechanical characteristics of annulus fibrosus tissue have restricted progress.
34918475	8	48	theme	mechanical	1782:1791	arg1	function					1793:1800	mechanical function	1782:1800	mechanical function	1782:1800	Our findings provide a theoretical and practical basis for the development of bionic annulus fibrosus tissue that closely mimics the biological properties, mechanical function, and matrix composition of native tissue.
34918475	6	49	theme	type	1357:1360	arg1	contents					1378:1385	collagen type II and aggrecan contents	1348:1385	collagen type II and aggrecan contents	1348:1385	Regarding extracellular matrix secretion, collagen type I content gradually increased with substrate elasticity, while collagen type II and aggrecan contents decreased.
34918475	4	50	theme	fibrous	878:884	arg1	scaffolds					886:894	DAFM/PECUU-blended fibrous scaffolds	859:894	DAFM/PECUU-blended fibrous scaffolds with elasticities approximating that of native inner and outer annulus fibrosus tissue	859:981	In this study, we used a coaxial electrospinning method to synthesize DAFM/PECUU-blended fibrous scaffolds with elasticities approximating that of native inner and outer annulus fibrosus tissue.
34918475	6	51	theme	matrix	1253:1258	arg1	secretion					1260:1268	extracellular matrix secretion	1239:1268	extracellular matrix secretion	1239:1268	Regarding extracellular matrix secretion, collagen type I content gradually increased with substrate elasticity, while collagen type II and aggrecan contents decreased.
34918475	7	52	theme	DAFM/PECUU-blended	1528:1545	arg1	scaffolds					1555:1563	DAFM/PECUU-blended fibrous scaffolds	1528:1563	DAFM/PECUU-blended fibrous scaffolds	1528:1563	In vivo evaluations employing magnetic resonance imaging, hematoxylin and eosin staining, and immunohistochemistry indicated that DAFM/PECUU-blended fibrous scaffolds could effectively repair defects of annulus fibrosus tissue.
34918475	4	53	theme	fibrosus	967:974	arg1	tissue					976:981	native inner and outer annulus fibrosus tissue	936:981	native inner and outer annulus fibrosus tissue	936:981	In this study, we used a coaxial electrospinning method to synthesize DAFM/PECUU-blended fibrous scaffolds with elasticities approximating that of native inner and outer annulus fibrosus tissue.
34918475	0	54	theme	fibrous	147:153	arg1	scaffolds					155:163	fibrous scaffolds	147:163	fibrous scaffolds with varying elastic moduli	147:191	The experimental study of regeneration of annulus fibrosus using decellularized annulus fibrosus matrix/poly(ether carbonate urethane)urea-blended fibrous scaffolds with varying elastic moduli.
34918475	3	55	theme	Decellularized	621:634	arg1	matrix					653:658	Decellularized annulus fibrosus matrix	621:658	Decellularized annulus fibrosus matrix (DAFM)	621:665	Decellularized annulus fibrosus matrix (DAFM) has good biocompatibility and biodegradability, making it suitable for cell adhesion, proliferation, and differentiation.
34918475	3	55	theme	Decellularized	621:634	arg1	DAFM					661:664	DAFM	661:664	DAFM	661:664	Decellularized annulus fibrosus matrix (DAFM) has good biocompatibility and biodegradability, making it suitable for cell adhesion, proliferation, and differentiation.
34918475	6	56	theme	substrate	1320:1328	arg1	elasticity					1330:1339	substrate elasticity	1320:1339	substrate elasticity	1320:1339	Regarding extracellular matrix secretion, collagen type I content gradually increased with substrate elasticity, while collagen type II and aggrecan contents decreased.
34918475	4	57	theme	inner	943:947	arg1	tissue					976:981	native inner and outer annulus fibrosus tissue	936:981	native inner and outer annulus fibrosus tissue	936:981	In this study, we used a coaxial electrospinning method to synthesize DAFM/PECUU-blended fibrous scaffolds with elasticities approximating that of native inner and outer annulus fibrosus tissue.
34918475	0	58	theme	elastic	178:184	arg1	moduli					186:191	varying elastic moduli	170:191	varying elastic moduli	170:191	The experimental study of regeneration of annulus fibrosus using decellularized annulus fibrosus matrix/poly(ether carbonate urethane)urea-blended fibrous scaffolds with varying elastic moduli.
34918475	5	59	theme	scaffold	1125:1132	arg1	material					1134:1141	the scaffold material	1121:1141	the scaffold material	1121:1141	AFSCs cultured on DAFM/PECUU-blended fibrous scaffolds exhibited increased collagen type I gene expression with increasing elasticity of the scaffold material; notably, collagen type II and aggrecan gene expression exhibited the opposite trend.
34918475	4	60	theme	outer	953:957	arg1	tissue					976:981	native inner and outer annulus fibrosus tissue	936:981	native inner and outer annulus fibrosus tissue	936:981	In this study, we used a coaxial electrospinning method to synthesize DAFM/PECUU-blended fibrous scaffolds with elasticities approximating that of native inner and outer annulus fibrosus tissue.
34918475	0	61	theme	decellularized	65:78	arg1	urethane					125:132	ether carbonate urethane	109:132	ether carbonate urethane	109:132	The experimental study of regeneration of annulus fibrosus using decellularized annulus fibrosus matrix/poly(ether carbonate urethane)urea-blended fibrous scaffolds with varying elastic moduli.
34918475	0	61	theme	decellularized	65:78	arg1	matrix/poly					97:107	decellularized annulus fibrosus matrix/poly	65:107	decellularized annulus fibrosus matrix/poly(ether carbonate urethane)	65:133	The experimental study of regeneration of annulus fibrosus using decellularized annulus fibrosus matrix/poly(ether carbonate urethane)urea-blended fibrous scaffolds with varying elastic moduli.
34918475	3	62	theme	fibrosus	644:651	arg1	matrix					653:658	Decellularized annulus fibrosus matrix	621:658	Decellularized annulus fibrosus matrix (DAFM)	621:665	Decellularized annulus fibrosus matrix (DAFM) has good biocompatibility and biodegradability, making it suitable for cell adhesion, proliferation, and differentiation.
34918475	3	62	theme	fibrosus	644:651	arg1	DAFM					661:664	DAFM	661:664	DAFM	661:664	Decellularized annulus fibrosus matrix (DAFM) has good biocompatibility and biodegradability, making it suitable for cell adhesion, proliferation, and differentiation.
34918475	6	63	theme	type	1280:1283	arg1	content					1287:1293	collagen type I content	1271:1293	collagen type I content	1271:1293	Regarding extracellular matrix secretion, collagen type I content gradually increased with substrate elasticity, while collagen type II and aggrecan contents decreased.
34918475	0	64	theme	fibrosus	88:95	arg1	urethane					125:132	ether carbonate urethane	109:132	ether carbonate urethane	109:132	The experimental study of regeneration of annulus fibrosus using decellularized annulus fibrosus matrix/poly(ether carbonate urethane)urea-blended fibrous scaffolds with varying elastic moduli.
34918475	0	64	theme	fibrosus	88:95	arg1	matrix/poly					97:107	decellularized annulus fibrosus matrix/poly	65:107	decellularized annulus fibrosus matrix/poly(ether carbonate urethane)	65:133	The experimental study of regeneration of annulus fibrosus using decellularized annulus fibrosus matrix/poly(ether carbonate urethane)urea-blended fibrous scaffolds with varying elastic moduli.
34918475	2	65	theme	carbonate	588:596	arg1	urethane					598:605	ether carbonate urethane	582:605	ether carbonate urethane	582:605	Differentiation of annulus fibrosus-derived stem cells (AFSCs) can be regulated by the elasticity of substrates such as poly(ether carbonate urethane)urea (PECUU).
34918475	2	65	theme	carbonate	588:596	arg1	poly					577:580	poly	577:580	poly(ether carbonate urethane)urea (PECUU)	577:618	Differentiation of annulus fibrosus-derived stem cells (AFSCs) can be regulated by the elasticity of substrates such as poly(ether carbonate urethane)urea (PECUU).
34918475	8	66	theme	theoretical	1649:1659	arg1	basis					1675:1679	a theoretical and practical basis	1647:1679	a theoretical and practical basis for the development of bionic annulus fibrosus tissue that closely mimics the biological properties, mechanical function, and matrix composition of native tissue	1647:1841	Our findings provide a theoretical and practical basis for the development of bionic annulus fibrosus tissue that closely mimics the biological properties, mechanical function, and matrix composition of native tissue.
34918475	5	67	theme	opposite	1213:1220	arg1	trend					1222:1226	the opposite trend	1209:1226	the opposite trend	1209:1226	AFSCs cultured on DAFM/PECUU-blended fibrous scaffolds exhibited increased collagen type I gene expression with increasing elasticity of the scaffold material; notably, collagen type II and aggrecan gene expression exhibited the opposite trend.
34918475	8	68	theme	practical	1665:1673	arg1	basis					1675:1679	a theoretical and practical basis	1647:1679	a theoretical and practical basis for the development of bionic annulus fibrosus tissue that closely mimics the biological properties, mechanical function, and matrix composition of native tissue	1647:1841	Our findings provide a theoretical and practical basis for the development of bionic annulus fibrosus tissue that closely mimics the biological properties, mechanical function, and matrix composition of native tissue.
34918475	0	69	theme	carbonate	115:123	arg1	urethane					125:132	ether carbonate urethane	109:132	ether carbonate urethane	109:132	The experimental study of regeneration of annulus fibrosus using decellularized annulus fibrosus matrix/poly(ether carbonate urethane)urea-blended fibrous scaffolds with varying elastic moduli.
34918475	0	69	theme	carbonate	115:123	arg1	matrix/poly					97:107	decellularized annulus fibrosus matrix/poly	65:107	decellularized annulus fibrosus matrix/poly(ether carbonate urethane)	65:133	The experimental study of regeneration of annulus fibrosus using decellularized annulus fibrosus matrix/poly(ether carbonate urethane)urea-blended fibrous scaffolds with varying elastic moduli.
34918475	1	70	theme	annulus	407:413	arg1	tissue					424:429	annulus fibrosus tissue	407:429	annulus fibrosus tissue	407:429	Although tissue engineering has attracted increasing attention for the treatment of degenerative intervertebral disc disease, the biochemical properties, structural organization, and mechanical characteristics of annulus fibrosus tissue have restricted progress.
34918475	5	71	theme	increased	1049:1057	arg1	expression					1080:1089	increased collagen type I gene expression	1049:1089	increased collagen type I gene expression	1049:1089	AFSCs cultured on DAFM/PECUU-blended fibrous scaffolds exhibited increased collagen type I gene expression with increasing elasticity of the scaffold material; notably, collagen type II and aggrecan gene expression exhibited the opposite trend.
34918475	7	72	theme	magnetic	1428:1435	arg1	imaging					1447:1453	magnetic resonance imaging	1428:1453	magnetic resonance imaging	1428:1453	In vivo evaluations employing magnetic resonance imaging, hematoxylin and eosin staining, and immunohistochemistry indicated that DAFM/PECUU-blended fibrous scaffolds could effectively repair defects of annulus fibrosus tissue.
34918475	2	73	theme	substrates	558:567	arg1	elasticity					544:553	the elasticity	540:553	the elasticity of substrates such as poly(ether carbonate urethane)urea (PECUU)	540:618	Differentiation of annulus fibrosus-derived stem cells (AFSCs) can be regulated by the elasticity of substrates such as poly(ether carbonate urethane)urea (PECUU).
34918475	0	74	theme	experimental	4:15	arg1	study					17:21	The experimental study	0:21	The experimental study of regeneration of annulus fibrosus using decellularized annulus fibrosus matrix/poly(ether carbonate urethane)	0:133	The experimental study of regeneration of annulus fibrosus using decellularized annulus fibrosus matrix/poly(ether carbonate urethane)urea-blended fibrous scaffolds with varying elastic moduli.
34918475	5	75	theme	gene	1183:1186	arg1	expression					1188:1197	aggrecan gene expression	1174:1197	aggrecan gene expression	1174:1197	AFSCs cultured on DAFM/PECUU-blended fibrous scaffolds exhibited increased collagen type I gene expression with increasing elasticity of the scaffold material; notably, collagen type II and aggrecan gene expression exhibited the opposite trend.
34918475	7	76	dep	In	1398:1399	arg1	vivo					1401:1404	vivo	1401:1404	vivo	1401:1404	In vivo evaluations employing magnetic resonance imaging, hematoxylin and eosin staining, and immunohistochemistry indicated that DAFM/PECUU-blended fibrous scaffolds could effectively repair defects of annulus fibrosus tissue.
34918475	5	77	theme	DAFM/PECUU-blended	1002:1019	arg1	scaffolds					1029:1037	DAFM/PECUU-blended fibrous scaffolds	1002:1037	DAFM/PECUU-blended fibrous scaffolds	1002:1037	AFSCs cultured on DAFM/PECUU-blended fibrous scaffolds exhibited increased collagen type I gene expression with increasing elasticity of the scaffold material; notably, collagen type II and aggrecan gene expression exhibited the opposite trend.
34918475	7	78	theme	In	1398:1399	arg1	evaluations					1406:1416	In vivo evaluations	1398:1416	In vivo evaluations employing magnetic resonance imaging, hematoxylin and eosin staining, and immunohistochemistry	1398:1511	In vivo evaluations employing magnetic resonance imaging, hematoxylin and eosin staining, and immunohistochemistry indicated that DAFM/PECUU-blended fibrous scaffolds could effectively repair defects of annulus fibrosus tissue.
34918475	2	79	theme	stem	501:504	arg1	AFSCs					513:517	AFSCs	513:517	AFSCs	513:517	Differentiation of annulus fibrosus-derived stem cells (AFSCs) can be regulated by the elasticity of substrates such as poly(ether carbonate urethane)urea (PECUU).
34918475	2	79	theme	stem	501:504	arg1	cells					506:510	annulus fibrosus-derived stem cells	476:510	annulus fibrosus-derived stem cells (AFSCs)	476:518	Differentiation of annulus fibrosus-derived stem cells (AFSCs) can be regulated by the elasticity of substrates such as poly(ether carbonate urethane)urea (PECUU).
34918475	8	80	theme	bionic	1704:1709	arg1	tissue					1728:1733	bionic annulus fibrosus tissue	1704:1733	bionic annulus fibrosus tissue that closely mimics the biological properties, mechanical function, and matrix composition of native tissue	1704:1841	Our findings provide a theoretical and practical basis for the development of bionic annulus fibrosus tissue that closely mimics the biological properties, mechanical function, and matrix composition of native tissue.
34918475	3	81	theme	good	671:674	arg1	biocompatibility					676:691	good biocompatibility	671:691	good biocompatibility	671:691	Decellularized annulus fibrosus matrix (DAFM) has good biocompatibility and biodegradability, making it suitable for cell adhesion, proliferation, and differentiation.
34918475	2	82	theme	annulus	476:482	arg1	AFSCs					513:517	AFSCs	513:517	AFSCs	513:517	Differentiation of annulus fibrosus-derived stem cells (AFSCs) can be regulated by the elasticity of substrates such as poly(ether carbonate urethane)urea (PECUU).
34918475	2	82	theme	annulus	476:482	arg1	cells					506:510	annulus fibrosus-derived stem cells	476:510	annulus fibrosus-derived stem cells (AFSCs)	476:518	Differentiation of annulus fibrosus-derived stem cells (AFSCs) can be regulated by the elasticity of substrates such as poly(ether carbonate urethane)urea (PECUU).
34918475	1	83	theme	biochemical	324:334	arg1	properties					336:345	the biochemical properties	320:345	the biochemical properties	320:345	Although tissue engineering has attracted increasing attention for the treatment of degenerative intervertebral disc disease, the biochemical properties, structural organization, and mechanical characteristics of annulus fibrosus tissue have restricted progress.
34918475	8	84	theme	fibrosus	1719:1726	arg1	tissue					1728:1733	bionic annulus fibrosus tissue	1704:1733	bionic annulus fibrosus tissue that closely mimics the biological properties, mechanical function, and matrix composition of native tissue	1704:1841	Our findings provide a theoretical and practical basis for the development of bionic annulus fibrosus tissue that closely mimics the biological properties, mechanical function, and matrix composition of native tissue.
34918475	0	85	theme	fibrosus	50:57	arg1	regeneration					26:37	regeneration	26:37	regeneration of annulus fibrosus using decellularized annulus fibrosus matrix/poly(ether carbonate urethane)	26:133	The experimental study of regeneration of annulus fibrosus using decellularized annulus fibrosus matrix/poly(ether carbonate urethane)urea-blended fibrous scaffolds with varying elastic moduli.
34918475	5	86	theme	aggrecan	1174:1181	arg1	expression					1188:1197	aggrecan gene expression	1174:1197	aggrecan gene expression	1174:1197	AFSCs cultured on DAFM/PECUU-blended fibrous scaffolds exhibited increased collagen type I gene expression with increasing elasticity of the scaffold material; notably, collagen type II and aggrecan gene expression exhibited the opposite trend.
34918475	5	87	theme	type	1068:1071	arg1	expression					1080:1089	increased collagen type I gene expression	1049:1089	increased collagen type I gene expression	1049:1089	AFSCs cultured on DAFM/PECUU-blended fibrous scaffolds exhibited increased collagen type I gene expression with increasing elasticity of the scaffold material; notably, collagen type II and aggrecan gene expression exhibited the opposite trend.
34918475	2	88	link	fibrosus-derived	484:499	arg1	AFSCs					513:517	AFSCs	513:517	AFSCs	513:517	Differentiation of annulus fibrosus-derived stem cells (AFSCs) can be regulated by the elasticity of substrates such as poly(ether carbonate urethane)urea (PECUU).
34918475	2	88	link	fibrosus-derived	484:499	arg1	cells					506:510	annulus fibrosus-derived stem cells	476:510	annulus fibrosus-derived stem cells (AFSCs)	476:518	Differentiation of annulus fibrosus-derived stem cells (AFSCs) can be regulated by the elasticity of substrates such as poly(ether carbonate urethane)urea (PECUU).
34918475	5	89	theme	gene	1075:1078	arg1	expression					1080:1089	increased collagen type I gene expression	1049:1089	increased collagen type I gene expression	1049:1089	AFSCs cultured on DAFM/PECUU-blended fibrous scaffolds exhibited increased collagen type I gene expression with increasing elasticity of the scaffold material; notably, collagen type II and aggrecan gene expression exhibited the opposite trend.
34918475	8	90	theme	biological	1759:1768	arg1	properties					1770:1779	the biological properties	1755:1779	the biological properties	1755:1779	Our findings provide a theoretical and practical basis for the development of bionic annulus fibrosus tissue that closely mimics the biological properties, mechanical function, and matrix composition of native tissue.
34918475	4	91	theme	coaxial	814:820	arg1	method					838:843	a coaxial electrospinning method	812:843	a coaxial electrospinning method	812:843	In this study, we used a coaxial electrospinning method to synthesize DAFM/PECUU-blended fibrous scaffolds with elasticities approximating that of native inner and outer annulus fibrosus tissue.
34918475	8	92	theme	native	1829:1834	arg1	tissue					1836:1841	native tissue	1829:1841	native tissue	1829:1841	Our findings provide a theoretical and practical basis for the development of bionic annulus fibrosus tissue that closely mimics the biological properties, mechanical function, and matrix composition of native tissue.
33711373	5	0	theme	electron	740:747	arg1	FESEM					761:765	FESEM	761:765	FESEM	761:765	Electrospun nanofibers were then characterized using field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), water contact angle, degradation studies, tensile tests, electrical resistance measurement and cell viability assay.
33711373	5	0	theme	electron	740:747	arg1	microscopy					749:758	field emission scanning electron microscopy	716:758	field emission scanning electron microscopy (FESEM)	716:766	Electrospun nanofibers were then characterized using field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), water contact angle, degradation studies, tensile tests, electrical resistance measurement and cell viability assay.
33711373	4	1	theme	Polyurethane	470:481	arg1	Cs					498:499	Cs	498:499	Cs	498:499	A variety of Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation were fabricated to structurally mimic the extracellular matrix (ECM).
33711373	4	1	theme	Polyurethane	470:481	arg1	/Chitosan					487:495	Polyurethane (PU)/Chitosan	470:495	Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation	470:591	A variety of Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation were fabricated to structurally mimic the extracellular matrix (ECM).
33711373	7	2	theme	fabricated	1085:1094	arg1	scaffolds					1128:1136	fabricated PU/Cs/CNT composite nanofibrous scaffolds	1085:1136	fabricated PU/Cs/CNT composite nanofibrous scaffolds	1085:1136	The results revealed that fabricated PU/Cs/CNT composite nanofibrous scaffolds were electro-conductive and aligned nanofibers could be considered as promising scaffolds with nano-scale features for regeneration of infarcted myocardium.
33711373	3	3	from	acid	415:418	arg1	series					355:360	a series	353:360	a series of polyurethane solutions (5-7%wt) in aqueous acetic acid	353:418	In this study, a series of polyurethane solutions (5-7%wt) in aqueous acetic acid were prepared using electrospinning.
33711373	1	4	theme	failure	183:189	arg1	worldwide					196:204	heart failure (HF) worldwide	177:204	heart failure (HF) worldwide	177:204	Myocardial infarction of cardiomyocytes is a leading cause of heart failure (HF) worldwide.
33711373	4	5	theme	composite	525:533	arg1	scaffolds					547:555	Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds	470:555	Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation	470:591	A variety of Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation were fabricated to structurally mimic the extracellular matrix (ECM).
33711373	7	6	theme	promising	1208:1216	arg1	scaffolds					1218:1226	promising scaffolds	1208:1226	promising scaffolds with nano-scale features for regeneration of infarcted myocardium	1208:1292	The results revealed that fabricated PU/Cs/CNT composite nanofibrous scaffolds were electro-conductive and aligned nanofibers could be considered as promising scaffolds with nano-scale features for regeneration of infarcted myocardium.
33711373	7	6	theme	promising	1208:1216	arg1	nanofibers					1174:1183	aligned nanofibers	1166:1183	aligned nanofibers	1166:1183	The results revealed that fabricated PU/Cs/CNT composite nanofibrous scaffolds were electro-conductive and aligned nanofibers could be considered as promising scaffolds with nano-scale features for regeneration of infarcted myocardium.
33711373	7	7	theme	PU/Cs/CNT	1096:1104	arg1	scaffolds					1128:1136	fabricated PU/Cs/CNT composite nanofibrous scaffolds	1085:1136	fabricated PU/Cs/CNT composite nanofibrous scaffolds	1085:1136	The results revealed that fabricated PU/Cs/CNT composite nanofibrous scaffolds were electro-conductive and aligned nanofibers could be considered as promising scaffolds with nano-scale features for regeneration of infarcted myocardium.
33711373	7	8	theme	composite	1106:1114	arg1	scaffolds					1128:1136	fabricated PU/Cs/CNT composite nanofibrous scaffolds	1085:1136	fabricated PU/Cs/CNT composite nanofibrous scaffolds	1085:1136	The results revealed that fabricated PU/Cs/CNT composite nanofibrous scaffolds were electro-conductive and aligned nanofibers could be considered as promising scaffolds with nano-scale features for regeneration of infarcted myocardium.
33711373	7	9	theme	myocardium	1283:1292	arg1	regeneration					1257:1268	regeneration	1257:1268	regeneration of infarcted myocardium	1257:1292	The results revealed that fabricated PU/Cs/CNT composite nanofibrous scaffolds were electro-conductive and aligned nanofibers could be considered as promising scaffolds with nano-scale features for regeneration of infarcted myocardium.
33711373	4	10	theme	PU	484:485	arg1	Cs					498:499	Cs	498:499	Cs	498:499	A variety of Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation were fabricated to structurally mimic the extracellular matrix (ECM).
33711373	4	10	theme	PU	484:485	arg1	/Chitosan					487:495	Polyurethane (PU)/Chitosan	470:495	Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation	470:591	A variety of Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation were fabricated to structurally mimic the extracellular matrix (ECM).
33711373	5	11	theme	resistance	902:911	arg1	measurement					913:923	electrical resistance measurement	891:923	electrical resistance measurement	891:923	Electrospun nanofibers were then characterized using field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), water contact angle, degradation studies, tensile tests, electrical resistance measurement and cell viability assay.
33711373	5	12	theme	electrical	891:900	arg1	measurement					913:923	electrical resistance measurement	891:923	electrical resistance measurement	891:923	Electrospun nanofibers were then characterized using field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), water contact angle, degradation studies, tensile tests, electrical resistance measurement and cell viability assay.
33711373	4	13	theme	scaffolds	547:555	arg1	variety					459:465	A variety	457:465	A variety of Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation	457:591	A variety of Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation were fabricated to structurally mimic the extracellular matrix (ECM).
33711373	4	13	theme	scaffolds	547:555	arg1	scaffolds					547:555	Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds	470:555	Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation	470:591	A variety of Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation were fabricated to structurally mimic the extracellular matrix (ECM).
33711373	5	14	theme	tensile	876:882	arg1	tests					884:888	tensile tests	876:888	tensile tests	876:888	Electrospun nanofibers were then characterized using field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), water contact angle, degradation studies, tensile tests, electrical resistance measurement and cell viability assay.
33711373	7	15	with	scaffolds	1218:1226	arg1	features					1244:1251	nano-scale features	1233:1251	nano-scale features for regeneration of infarcted myocardium	1233:1292	The results revealed that fabricated PU/Cs/CNT composite nanofibrous scaffolds were electro-conductive and aligned nanofibers could be considered as promising scaffolds with nano-scale features for regeneration of infarcted myocardium.
33711373	5	16	theme	contact	840:846	arg1	angle					848:852	water contact angle	834:852	water contact angle	834:852	Electrospun nanofibers were then characterized using field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), water contact angle, degradation studies, tensile tests, electrical resistance measurement and cell viability assay.
33711373	4	17	theme	nanofibrous	535:545	arg1	scaffolds					547:555	Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds	470:555	Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation	470:591	A variety of Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation were fabricated to structurally mimic the extracellular matrix (ECM).
33711373	5	18	theme	transmission	769:780	arg1	TEM					803:805	TEM	803:805	TEM	803:805	Electrospun nanofibers were then characterized using field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), water contact angle, degradation studies, tensile tests, electrical resistance measurement and cell viability assay.
33711373	5	18	theme	transmission	769:780	arg1	microscopy					791:800	transmission electron microscopy	769:800	transmission electron microscopy (TEM)	769:806	Electrospun nanofibers were then characterized using field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), water contact angle, degradation studies, tensile tests, electrical resistance measurement and cell viability assay.
33711373	6	19	theme	random	987:992	arg1	scaffolds					1018:1026	electrospun random and aligned nanofibrous scaffolds	975:1026	electrospun random and aligned nanofibrous scaffolds with H9C2 Cells	975:1042	The biocompatibility of electrospun random and aligned nanofibrous scaffolds with H9C2 Cells was confirmed.
33711373	4	20	theme	extracellular	635:647	arg1	ECM					657:659	ECM	657:659	ECM	657:659	A variety of Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation were fabricated to structurally mimic the extracellular matrix (ECM).
33711373	4	20	theme	extracellular	635:647	arg1	matrix					649:654	the extracellular matrix	631:654	the extracellular matrix (ECM)	631:660	A variety of Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation were fabricated to structurally mimic the extracellular matrix (ECM).
33711373	3	21	theme	acetic	408:413	arg1	acid					415:418	aqueous acetic acid	400:418	aqueous acetic acid	400:418	In this study, a series of polyurethane solutions (5-7%wt) in aqueous acetic acid were prepared using electrospinning.
33711373	0	22	theme	scaffold	74:81	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of polyurethane/chitosan/CNT nanofibrous scaffold for cardiac tissue engineering.
33711373	0	22	theme	scaffold	74:81	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of polyurethane/chitosan/CNT nanofibrous scaffold for cardiac tissue engineering.
33711373	3	23	from	solutions	378:386	arg1	acid					415:418	aqueous acetic acid	400:418	aqueous acetic acid	400:418	In this study, a series of polyurethane solutions (5-7%wt) in aqueous acetic acid were prepared using electrospinning.
33711373	5	24	theme	cell	929:932	arg1	assay					944:948	cell viability assay	929:948	cell viability assay	929:948	Electrospun nanofibers were then characterized using field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), water contact angle, degradation studies, tensile tests, electrical resistance measurement and cell viability assay.
33711373	3	25	theme	solutions	378:386	arg1	series					355:360	a series	353:360	a series of polyurethane solutions (5-7%wt) in aqueous acetic acid	353:418	In this study, a series of polyurethane solutions (5-7%wt) in aqueous acetic acid were prepared using electrospinning.
33711373	5	26	theme	emission	722:729	arg1	FESEM					761:765	FESEM	761:765	FESEM	761:765	Electrospun nanofibers were then characterized using field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), water contact angle, degradation studies, tensile tests, electrical resistance measurement and cell viability assay.
33711373	5	26	theme	emission	722:729	arg1	microscopy					749:758	field emission scanning electron microscopy	716:758	field emission scanning electron microscopy (FESEM)	716:766	Electrospun nanofibers were then characterized using field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), water contact angle, degradation studies, tensile tests, electrical resistance measurement and cell viability assay.
33711373	1	27	theme	Myocardial	115:124	arg1	infarction					126:135	Myocardial infarction	115:135	Myocardial infarction of cardiomyocytes	115:153	Myocardial infarction of cardiomyocytes is a leading cause of heart failure (HF) worldwide.
33711373	1	27	theme	Myocardial	115:124	arg1	cause					168:172	a leading cause	158:172	a leading cause of heart failure (HF) worldwide	158:204	Myocardial infarction of cardiomyocytes is a leading cause of heart failure (HF) worldwide.
33711373	6	28	theme	H9C2	1033:1036	arg1	Cells					1038:1042	H9C2 Cells	1033:1042	H9C2 Cells	1033:1042	The biocompatibility of electrospun random and aligned nanofibrous scaffolds with H9C2 Cells was confirmed.
33711373	1	29	theme	worldwide	196:204	arg1	infarction					126:135	Myocardial infarction	115:135	Myocardial infarction of cardiomyocytes	115:153	Myocardial infarction of cardiomyocytes is a leading cause of heart failure (HF) worldwide.
33711373	1	29	theme	worldwide	196:204	arg1	cause					168:172	a leading cause	158:172	a leading cause of heart failure (HF) worldwide	158:204	Myocardial infarction of cardiomyocytes is a leading cause of heart failure (HF) worldwide.
33711373	5	30	theme	electron	782:789	arg1	TEM					803:805	TEM	803:805	TEM	803:805	Electrospun nanofibers were then characterized using field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), water contact angle, degradation studies, tensile tests, electrical resistance measurement and cell viability assay.
33711373	5	30	theme	electron	782:789	arg1	microscopy					791:800	transmission electron microscopy	769:800	transmission electron microscopy (TEM)	769:806	Electrospun nanofibers were then characterized using field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), water contact angle, degradation studies, tensile tests, electrical resistance measurement and cell viability assay.
33711373	4	31	theme	nanotubes	509:517	arg1	scaffolds					547:555	Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds	470:555	Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation	470:591	A variety of Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation were fabricated to structurally mimic the extracellular matrix (ECM).
33711373	3	32	theme	aqueous	400:406	arg1	acid					415:418	aqueous acetic acid	400:418	aqueous acetic acid	400:418	In this study, a series of polyurethane solutions (5-7%wt) in aqueous acetic acid were prepared using electrospinning.
33711373	4	33	theme	/carbon	501:507	arg1	scaffolds					547:555	Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds	470:555	Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation	470:591	A variety of Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation were fabricated to structurally mimic the extracellular matrix (ECM).
33711373	2	34	theme	bioactive	304:312	arg1	scaffold					314:321	a bioactive scaffold	302:321	a bioactive scaffold	302:321	Since heart has very limited regeneration capacity, cardiac tissue engineering (TE) to produce a bioactive scaffold is considered.
33711373	6	35	theme	scaffolds	1018:1026	arg1	biocompatibility					955:970	The biocompatibility	951:970	The biocompatibility of electrospun random and aligned nanofibrous scaffolds with H9C2 Cells	951:1042	The biocompatibility of electrospun random and aligned nanofibrous scaffolds with H9C2 Cells was confirmed.
33711373	3	36	theme	polyurethane	365:376	arg1	solutions					378:386	polyurethane solutions	365:386	polyurethane solutions (5-7%wt) in aqueous acetic acid	365:418	In this study, a series of polyurethane solutions (5-7%wt) in aqueous acetic acid were prepared using electrospinning.
33711373	3	36	theme	polyurethane	365:376	arg1	wt					393:394	5-7%wt	389:394	5-7%wt	389:394	In this study, a series of polyurethane solutions (5-7%wt) in aqueous acetic acid were prepared using electrospinning.
33711373	4	37	theme	CNT	520:522	arg1	scaffolds					547:555	Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds	470:555	Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation	470:591	A variety of Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation were fabricated to structurally mimic the extracellular matrix (ECM).
33711373	5	38	theme	Electrospun	663:673	arg1	nanofibers					675:684	Electrospun nanofibers	663:684	Electrospun nanofibers	663:684	Electrospun nanofibers were then characterized using field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), water contact angle, degradation studies, tensile tests, electrical resistance measurement and cell viability assay.
33711373	1	39	theme	cardiomyocytes	140:153	arg1	infarction					126:135	Myocardial infarction	115:135	Myocardial infarction of cardiomyocytes	115:153	Myocardial infarction of cardiomyocytes is a leading cause of heart failure (HF) worldwide.
33711373	1	39	theme	cardiomyocytes	140:153	arg1	cause					168:172	a leading cause	158:172	a leading cause of heart failure (HF) worldwide	158:204	Myocardial infarction of cardiomyocytes is a leading cause of heart failure (HF) worldwide.
33711373	6	40	theme	nanofibrous	1006:1016	arg1	scaffolds					1018:1026	electrospun random and aligned nanofibrous scaffolds	975:1026	electrospun random and aligned nanofibrous scaffolds with H9C2 Cells	975:1042	The biocompatibility of electrospun random and aligned nanofibrous scaffolds with H9C2 Cells was confirmed.
33711373	0	41	theme	nanofibrous	62:72	arg1	scaffold					74:81	polyurethane/chitosan/CNT nanofibrous scaffold	36:81	polyurethane/chitosan/CNT nanofibrous scaffold	36:81	Preparation and characterization of polyurethane/chitosan/CNT nanofibrous scaffold for cardiac tissue engineering.
33711373	6	42	theme	aligned	998:1004	arg1	scaffolds					1018:1026	electrospun random and aligned nanofibrous scaffolds	975:1026	electrospun random and aligned nanofibrous scaffolds with H9C2 Cells	975:1042	The biocompatibility of electrospun random and aligned nanofibrous scaffolds with H9C2 Cells was confirmed.
33711373	2	43	contain	has	219:221	arg1	heart					213:217	heart	213:217	heart	213:217	Since heart has very limited regeneration capacity, cardiac tissue engineering (TE) to produce a bioactive scaffold is considered.
33711373	2	43	contain	has	219:221	arg2	capacity					249:256	very limited regeneration capacity	223:256	very limited regeneration capacity	223:256	Since heart has very limited regeneration capacity, cardiac tissue engineering (TE) to produce a bioactive scaffold is considered.
33711373	0	44	theme	polyurethane/chitosan/CNT	36:60	arg1	scaffold					74:81	polyurethane/chitosan/CNT nanofibrous scaffold	36:81	polyurethane/chitosan/CNT nanofibrous scaffold	36:81	Preparation and characterization of polyurethane/chitosan/CNT nanofibrous scaffold for cardiac tissue engineering.
33711373	5	45	theme	field	716:720	arg1	FESEM					761:765	FESEM	761:765	FESEM	761:765	Electrospun nanofibers were then characterized using field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), water contact angle, degradation studies, tensile tests, electrical resistance measurement and cell viability assay.
33711373	5	45	theme	field	716:720	arg1	microscopy					749:758	field emission scanning electron microscopy	716:758	field emission scanning electron microscopy (FESEM)	716:766	Electrospun nanofibers were then characterized using field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), water contact angle, degradation studies, tensile tests, electrical resistance measurement and cell viability assay.
33711373	3	46	theme	5-7	389:391	arg1	solutions					378:386	polyurethane solutions	365:386	polyurethane solutions (5-7%wt) in aqueous acetic acid	365:418	In this study, a series of polyurethane solutions (5-7%wt) in aqueous acetic acid were prepared using electrospinning.
33711373	3	46	theme	5-7	389:391	arg1	wt					393:394	5-7%wt	389:394	5-7%wt	389:394	In this study, a series of polyurethane solutions (5-7%wt) in aqueous acetic acid were prepared using electrospinning.
33711373	4	47	theme	/Chitosan	487:495	arg1	scaffolds					547:555	Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds	470:555	Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation	470:591	A variety of Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation were fabricated to structurally mimic the extracellular matrix (ECM).
33711373	3	48	from	series	355:360	arg1	acid					415:418	aqueous acetic acid	400:418	aqueous acetic acid	400:418	In this study, a series of polyurethane solutions (5-7%wt) in aqueous acetic acid were prepared using electrospinning.
33711373	2	49	theme	regeneration	236:247	arg1	capacity					249:256	very limited regeneration capacity	223:256	very limited regeneration capacity	223:256	Since heart has very limited regeneration capacity, cardiac tissue engineering (TE) to produce a bioactive scaffold is considered.
33711373	3	50	theme	%	392:392	arg1	solutions					378:386	polyurethane solutions	365:386	polyurethane solutions (5-7%wt) in aqueous acetic acid	365:418	In this study, a series of polyurethane solutions (5-7%wt) in aqueous acetic acid were prepared using electrospinning.
33711373	3	50	theme	%	392:392	arg1	wt					393:394	5-7%wt	389:394	5-7%wt	389:394	In this study, a series of polyurethane solutions (5-7%wt) in aqueous acetic acid were prepared using electrospinning.
33711373	2	51	theme	limited	228:234	arg1	capacity					249:256	very limited regeneration capacity	223:256	very limited regeneration capacity	223:256	Since heart has very limited regeneration capacity, cardiac tissue engineering (TE) to produce a bioactive scaffold is considered.
33711373	5	52	theme	degradation	855:865	arg1	studies					867:873	degradation studies	855:873	degradation studies	855:873	Electrospun nanofibers were then characterized using field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), water contact angle, degradation studies, tensile tests, electrical resistance measurement and cell viability assay.
33711373	7	53	theme	nanofibrous	1116:1126	arg1	scaffolds					1128:1136	fabricated PU/Cs/CNT composite nanofibrous scaffolds	1085:1136	fabricated PU/Cs/CNT composite nanofibrous scaffolds	1085:1136	The results revealed that fabricated PU/Cs/CNT composite nanofibrous scaffolds were electro-conductive and aligned nanofibers could be considered as promising scaffolds with nano-scale features for regeneration of infarcted myocardium.
33711373	5	54	theme	viability	934:942	arg1	assay					944:948	cell viability assay	929:948	cell viability assay	929:948	Electrospun nanofibers were then characterized using field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), water contact angle, degradation studies, tensile tests, electrical resistance measurement and cell viability assay.
33711373	4	55	with	scaffolds	547:555	arg1	orientation					581:591	random and aligned orientation	562:591	orientation	581:591	A variety of Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation were fabricated to structurally mimic the extracellular matrix (ECM).
33711373	5	56	theme	scanning	731:738	arg1	FESEM					761:765	FESEM	761:765	FESEM	761:765	Electrospun nanofibers were then characterized using field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), water contact angle, degradation studies, tensile tests, electrical resistance measurement and cell viability assay.
33711373	5	56	theme	scanning	731:738	arg1	microscopy					749:758	field emission scanning electron microscopy	716:758	field emission scanning electron microscopy (FESEM)	716:766	Electrospun nanofibers were then characterized using field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), water contact angle, degradation studies, tensile tests, electrical resistance measurement and cell viability assay.
33711373	1	57	theme	leading	160:166	arg1	infarction					126:135	Myocardial infarction	115:135	Myocardial infarction of cardiomyocytes	115:153	Myocardial infarction of cardiomyocytes is a leading cause of heart failure (HF) worldwide.
33711373	1	57	theme	leading	160:166	arg1	cause					168:172	a leading cause	158:172	a leading cause of heart failure (HF) worldwide	158:204	Myocardial infarction of cardiomyocytes is a leading cause of heart failure (HF) worldwide.
33711373	4	58	theme	random	562:567	arg1	orientation					581:591	random and aligned orientation	562:591	orientation	581:591	A variety of Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation were fabricated to structurally mimic the extracellular matrix (ECM).
33711373	6	59	with	scaffolds	1018:1026	arg1	Cells					1038:1042	H9C2 Cells	1033:1042	H9C2 Cells	1033:1042	The biocompatibility of electrospun random and aligned nanofibrous scaffolds with H9C2 Cells was confirmed.
33711373	7	60	theme	aligned	1166:1172	arg1	scaffolds					1218:1226	promising scaffolds	1208:1226	promising scaffolds with nano-scale features for regeneration of infarcted myocardium	1208:1292	The results revealed that fabricated PU/Cs/CNT composite nanofibrous scaffolds were electro-conductive and aligned nanofibers could be considered as promising scaffolds with nano-scale features for regeneration of infarcted myocardium.
33711373	7	60	theme	aligned	1166:1172	arg1	nanofibers					1174:1183	aligned nanofibers	1166:1183	aligned nanofibers	1166:1183	The results revealed that fabricated PU/Cs/CNT composite nanofibrous scaffolds were electro-conductive and aligned nanofibers could be considered as promising scaffolds with nano-scale features for regeneration of infarcted myocardium.
33711373	5	61	theme	water	834:838	arg1	angle					848:852	water contact angle	834:852	water contact angle	834:852	Electrospun nanofibers were then characterized using field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), water contact angle, degradation studies, tensile tests, electrical resistance measurement and cell viability assay.
33711373	6	62	theme	electrospun	975:985	arg1	scaffolds					1018:1026	electrospun random and aligned nanofibrous scaffolds	975:1026	electrospun random and aligned nanofibrous scaffolds with H9C2 Cells	975:1042	The biocompatibility of electrospun random and aligned nanofibrous scaffolds with H9C2 Cells was confirmed.
33711373	0	63	theme	tissue	95:100	arg1	engineering					102:112	cardiac tissue engineering	87:112	cardiac tissue engineering	87:112	Preparation and characterization of polyurethane/chitosan/CNT nanofibrous scaffold for cardiac tissue engineering.
33711373	5	64	theme	X-ray	809:813	arg1	XRD					828:830	XRD	828:830	XRD	828:830	Electrospun nanofibers were then characterized using field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), water contact angle, degradation studies, tensile tests, electrical resistance measurement and cell viability assay.
33711373	5	64	theme	X-ray	809:813	arg1	diffraction					815:825	X-ray diffraction	809:825	X-ray diffraction (XRD)	809:831	Electrospun nanofibers were then characterized using field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), water contact angle, degradation studies, tensile tests, electrical resistance measurement and cell viability assay.
33711373	2	65	theme	tissue	267:272	arg1	TE					287:288	TE	287:288	TE	287:288	Since heart has very limited regeneration capacity, cardiac tissue engineering (TE) to produce a bioactive scaffold is considered.
33711373	2	65	theme	tissue	267:272	arg1	engineering					274:284	cardiac tissue engineering	259:284	cardiac tissue engineering (TE) to produce a bioactive scaffold	259:321	Since heart has very limited regeneration capacity, cardiac tissue engineering (TE) to produce a bioactive scaffold is considered.
33711373	7	66	theme	nano-scale	1233:1242	arg1	features					1244:1251	nano-scale features	1233:1251	nano-scale features for regeneration of infarcted myocardium	1233:1292	The results revealed that fabricated PU/Cs/CNT composite nanofibrous scaffolds were electro-conductive and aligned nanofibers could be considered as promising scaffolds with nano-scale features for regeneration of infarcted myocardium.
33711373	4	67	theme	aligned	573:579	arg1	orientation					581:591	random and aligned orientation	562:591	orientation	581:591	A variety of Polyurethane (PU)/Chitosan (Cs)/carbon nanotubes (CNT) composite nanofibrous scaffolds with random and aligned orientation were fabricated to structurally mimic the extracellular matrix (ECM).
33711373	0	68	theme	cardiac	87:93	arg1	engineering					102:112	cardiac tissue engineering	87:112	cardiac tissue engineering	87:112	Preparation and characterization of polyurethane/chitosan/CNT nanofibrous scaffold for cardiac tissue engineering.
33711373	2	69	theme	cardiac	259:265	arg1	TE					287:288	TE	287:288	TE	287:288	Since heart has very limited regeneration capacity, cardiac tissue engineering (TE) to produce a bioactive scaffold is considered.
33711373	2	69	theme	cardiac	259:265	arg1	engineering					274:284	cardiac tissue engineering	259:284	cardiac tissue engineering (TE) to produce a bioactive scaffold	259:321	Since heart has very limited regeneration capacity, cardiac tissue engineering (TE) to produce a bioactive scaffold is considered.
33711373	7	70	theme	infarcted	1273:1281	arg1	myocardium					1283:1292	infarcted myocardium	1273:1292	infarcted myocardium	1273:1292	The results revealed that fabricated PU/Cs/CNT composite nanofibrous scaffolds were electro-conductive and aligned nanofibers could be considered as promising scaffolds with nano-scale features for regeneration of infarcted myocardium.
33711373	1	71	theme	heart	177:181	arg1	HF					192:193	HF	192:193	HF	192:193	Myocardial infarction of cardiomyocytes is a leading cause of heart failure (HF) worldwide.
33711373	1	71	theme	heart	177:181	arg1	failure					183:189	heart failure	177:189	heart failure (HF) worldwide	177:204	Myocardial infarction of cardiomyocytes is a leading cause of heart failure (HF) worldwide.
32674247	13	0	theme	bladder	1621:1627	arg1	patients					1636:1643	bladder cancer patients	1621:1643	bladder cancer patients	1621:1643	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity were found in bladder cancer patients, which might be associated with inadequate fruit intake.
32674247	7	1	theme	D-lactic	706:713	arg1	acid					715:718	D-lactic acid	706:718	D-lactic acid	706:718	The concentrations of lipopolysaccharide and D-lactic acid in serum were determined by enzyme-linked immunosorbent assay kits.
32674247	13	2	located	found	1612:1616	arg2	CONCLUSIONS					1483:1493	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity	1483:1605	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity	1483:1605	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity were found in bladder cancer patients, which might be associated with inadequate fruit intake.
32674247	13	2	located	found	1612:1616	arg2	Dysbiosis					1495:1503	Dysbiosis	1495:1503	Dysbiosis of gut microbiota	1495:1521	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity were found in bladder cancer patients, which might be associated with inadequate fruit intake.
32674247	13	2	located	found	1612:1616	arg2	integrity					1597:1605	impaired intestinal structural integrity	1566:1605	impaired intestinal structural integrity	1566:1605	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity were found in bladder cancer patients, which might be associated with inadequate fruit intake.
32674247	13	2	located	found	1612:1616	arg1	patients					1636:1643	bladder cancer patients	1621:1643	bladder cancer patients	1621:1643	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity were found in bladder cancer patients, which might be associated with inadequate fruit intake.
32674247	4	3	theme	genomic	397:403	arg1	DNA					405:407	the total genomic DNA	387:407	the total genomic DNA extracted from human feces	387:434	Pyrosequencing of the total genomic DNA extracted from human feces was carried out by Illumina HiSeq 2000.
32674247	2	4	theme	diagnosed	242:250	arg1	patients					267:274	Newly diagnosed bladder cancer patients	236:274	Newly diagnosed bladder cancer patients	236:274	METHODS AND STUDY DESIGN Newly diagnosed bladder cancer patients were recruited.
32674247	12	5	theme	permeability	1428:1439	arg1	acid					1393:1396	D-lactic acid	1384:1396	D-lactic acid	1384:1396	The concentrations of lipopolysaccharide and D-lactic acid, two sensitive markers of gut permeability, were greater in bladder cancer patients.
32674247	12	5	theme	permeability	1428:1439	arg1	markers					1413:1419	two sensitive markers	1399:1419	two sensitive markers of gut permeability	1399:1439	The concentrations of lipopolysaccharide and D-lactic acid, two sensitive markers of gut permeability, were greater in bladder cancer patients.
32674247	6	6	theme	chain	583:587	arg1	acids					595:599	Fecal short chain fatty acids	571:599	Fecal short chain fatty acids contents	571:608	Fecal short chain fatty acids contents were measured by gas chromatography (GC) analysis.
32674247	12	7	theme	acid	1393:1396	arg1	greater					1447:1453	greater	1447:1453	greater	1447:1453	The concentrations of lipopolysaccharide and D-lactic acid, two sensitive markers of gut permeability, were greater in bladder cancer patients.
32674247	12	7	theme	acid	1393:1396	arg1	concentrations					1343:1356	The concentrations	1339:1356	The concentrations of lipopolysaccharide and D-lactic acid, two sensitive markers of gut permeability,	1339:1440	The concentrations of lipopolysaccharide and D-lactic acid, two sensitive markers of gut permeability, were greater in bladder cancer patients.
32674247	6	8	theme	Fecal	571:575	arg1	acids					595:599	Fecal short chain fatty acids	571:599	Fecal short chain fatty acids contents	571:608	Fecal short chain fatty acids contents were measured by gas chromatography (GC) analysis.
32674247	10	9	theme	fruit	1046:1050	arg1	intake					1052:1057	fruit intake	1046:1057	fruit intake (r=0.002, p<0.05 for domain bacteria; r=0.004, p<0.05 for Prevotella)	1046:1127	The numbers of domain bacteria and Prevotella were significantly and positively associated with fruit intake (r=0.002, p<0.05 for domain bacteria; r=0.004, p<0.05 for Prevotella).
32674247	1	10	theme	bacteria	174:181	arg1	changes					159:165	the changes	155:165	the changes of gut bacteria in bladder cancer patients	155:208	BACKGROUND AND OBJECTIVES This study aimed to explore the changes of gut bacteria in bladder cancer patients.
32674247	2	11	dep	METHODS	211:217	arg1	patients					267:274	Newly diagnosed bladder cancer patients	236:274	Newly diagnosed bladder cancer patients	236:274	METHODS AND STUDY DESIGN Newly diagnosed bladder cancer patients were recruited.
32674247	1	12	theme	bladder	186:192	arg1	patients					201:208	bladder cancer patients	186:208	bladder cancer patients	186:208	BACKGROUND AND OBJECTIVES This study aimed to explore the changes of gut bacteria in bladder cancer patients.
32674247	13	13	theme	intestinal	1575:1584	arg1	CONCLUSIONS					1483:1493	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity	1483:1605	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity	1483:1605	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity were found in bladder cancer patients, which might be associated with inadequate fruit intake.
32674247	13	13	theme	intestinal	1575:1584	arg1	integrity					1597:1605	impaired intestinal structural integrity	1566:1605	impaired intestinal structural integrity	1566:1605	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity were found in bladder cancer patients, which might be associated with inadequate fruit intake.
32674247	6	14	theme	chromatography	631:644	arg1	analysis					651:658	gas chromatography (GC) analysis	627:658	gas chromatography (GC) analysis	627:658	Fecal short chain fatty acids contents were measured by gas chromatography (GC) analysis.
32674247	13	15	theme	decreased	1524:1532	arg1	concentrations					1547:1560	decreased butyric acid concentrations	1524:1560	decreased butyric acid concentrations	1524:1560	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity were found in bladder cancer patients, which might be associated with inadequate fruit intake.
32674247	5	16	theme	target	495:500	arg1	DNA					502:504	target DNA	495:504	target DNA for bacteria	495:517	The copy number of target DNA for bacteria was determined by real-time quantitative PCR assay.
32674247	10	17	dep	intake	1052:1057	arg1	p<0.05					1069:1074	p<0.05	1069:1074	p<0.05	1069:1074	The numbers of domain bacteria and Prevotella were significantly and positively associated with fruit intake (r=0.002, p<0.05 for domain bacteria; r=0.004, p<0.05 for Prevotella).
32674247	10	17	dep	intake	1052:1057	arg1	r=0.002					1060:1066	r=0.002	1060:1066	r=0.002	1060:1066	The numbers of domain bacteria and Prevotella were significantly and positively associated with fruit intake (r=0.002, p<0.05 for domain bacteria; r=0.004, p<0.05 for Prevotella).
32674247	10	17	dep	intake	1052:1057	arg1	p<0.05					1106:1111	p<0.05	1106:1111	p<0.05	1106:1111	The numbers of domain bacteria and Prevotella were significantly and positively associated with fruit intake (r=0.002, p<0.05 for domain bacteria; r=0.004, p<0.05 for Prevotella).
32674247	4	18	theme	human	424:428	arg1	feces					430:434	human feces	424:434	human feces	424:434	Pyrosequencing of the total genomic DNA extracted from human feces was carried out by Illumina HiSeq 2000.
32674247	13	19	theme	acid	1542:1545	arg1	concentrations					1547:1560	decreased butyric acid concentrations	1524:1560	decreased butyric acid concentrations	1524:1560	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity were found in bladder cancer patients, which might be associated with inadequate fruit intake.
32674247	8	20	theme	healthy	841:847	arg1	controls					849:856	healthy controls	841:856	healthy controls	841:856	RESULTS Fruit intake was significantly lower than in healthy controls.
32674247	10	21	dep	r=0.002	1060:1066	arg1	r=0.004					1097:1103	r=0.004	1097:1103	r=0.004	1097:1103	The numbers of domain bacteria and Prevotella were significantly and positively associated with fruit intake (r=0.002, p<0.05 for domain bacteria; r=0.004, p<0.05 for Prevotella).
32674247	7	22	dep	enzyme-linked	748:760	arg1	immunosorbent					762:774	immunosorbent	762:774	immunosorbent	762:774	The concentrations of lipopolysaccharide and D-lactic acid in serum were determined by enzyme-linked immunosorbent assay kits.
32674247	8	23	theme	RESULTS	788:794	arg1	intake					802:807	RESULTS Fruit intake	788:807	RESULTS Fruit intake	788:807	RESULTS Fruit intake was significantly lower than in healthy controls.
32674247	0	24	theme	case-control	64:75	arg1	study					77:81	A case-control study	62:81	Gut microbial composition changes in bladder cancer patients: A case-control study in Harbin, China.	0:99	Gut microbial composition changes in bladder cancer patients: A case-control study in Harbin, China.
32674247	6	25	theme	acids	595:599	arg1	contents					601:608	Fecal short chain fatty acids contents	571:608	Fecal short chain fatty acids contents	571:608	Fecal short chain fatty acids contents were measured by gas chromatography (GC) analysis.
32674247	1	26	from	changes	159:165	arg1	patients					201:208	bladder cancer patients	186:208	bladder cancer patients	186:208	BACKGROUND AND OBJECTIVES This study aimed to explore the changes of gut bacteria in bladder cancer patients.
32674247	2	27	theme	cancer	260:265	arg1	patients					267:274	Newly diagnosed bladder cancer patients	236:274	Newly diagnosed bladder cancer patients	236:274	METHODS AND STUDY DESIGN Newly diagnosed bladder cancer patients were recruited.
32674247	5	28	theme	quantitative	547:558	arg1	assay					564:568	real-time quantitative PCR assay	537:568	real-time quantitative PCR assay	537:568	The copy number of target DNA for bacteria was determined by real-time quantitative PCR assay.
32674247	13	29	theme	gut	1508:1510	arg1	microbiota					1512:1521	gut microbiota	1508:1521	gut microbiota	1508:1521	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity were found in bladder cancer patients, which might be associated with inadequate fruit intake.
32674247	0	30	theme	Gut	0:2	arg1	changes					26:32	Gut microbial composition changes	0:32	Gut microbial composition changes in bladder cancer patients: A case-control study in Harbin, China.	0:99	Gut microbial composition changes in bladder cancer patients: A case-control study in Harbin, China.
32674247	13	31	theme	butyric	1534:1540	arg1	acid					1542:1545	butyric acid	1534:1545	decreased butyric acid concentrations	1524:1560	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity were found in bladder cancer patients, which might be associated with inadequate fruit intake.
32674247	0	32	theme	composition	14:24	arg1	changes					26:32	Gut microbial composition changes	0:32	Gut microbial composition changes in bladder cancer patients: A case-control study in Harbin, China.	0:99	Gut microbial composition changes in bladder cancer patients: A case-control study in Harbin, China.
32674247	13	33	theme	cancer	1629:1634	arg1	patients					1636:1643	bladder cancer patients	1621:1643	bladder cancer patients	1621:1643	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity were found in bladder cancer patients, which might be associated with inadequate fruit intake.
32674247	11	34	theme	cancer	1199:1204	arg1	patients					1206:1213	bladder cancer patients	1191:1213	bladder cancer patients	1191:1213	The concentration of butyric acid decreased significantly in bladder cancer patients, and the quantities of fecal butyric acid were significantly and positively associated with fruit intake (r=0.610, p<0.01).
32674247	13	35	theme	inadequate	1677:1686	arg1	intake					1694:1699	inadequate fruit intake	1677:1699	inadequate fruit intake	1677:1699	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity were found in bladder cancer patients, which might be associated with inadequate fruit intake.
32674247	7	36	theme	assay	776:780	arg1	kits					782:785	enzyme-linked immunosorbent assay kits	748:785	enzyme-linked immunosorbent assay kits	748:785	The concentrations of lipopolysaccharide and D-lactic acid in serum were determined by enzyme-linked immunosorbent assay kits.
32674247	10	37	theme	bacteria	972:979	arg1	numbers					954:960	The numbers	950:960	The numbers of domain bacteria and Prevotella	950:994	The numbers of domain bacteria and Prevotella were significantly and positively associated with fruit intake (r=0.002, p<0.05 for domain bacteria; r=0.004, p<0.05 for Prevotella).
32674247	7	38	link	enzyme-linked	748:760	arg1	kits					782:785	enzyme-linked immunosorbent assay kits	748:785	enzyme-linked immunosorbent assay kits	748:785	The concentrations of lipopolysaccharide and D-lactic acid in serum were determined by enzyme-linked immunosorbent assay kits.
32674247	11	39	dep	intake	1313:1318	arg1	p<0.01					1330:1335	p<0.01	1330:1335	p<0.01	1330:1335	The concentration of butyric acid decreased significantly in bladder cancer patients, and the quantities of fecal butyric acid were significantly and positively associated with fruit intake (r=0.610, p<0.01).
32674247	11	39	dep	intake	1313:1318	arg1	r=0.610					1321:1327	r=0.610	1321:1327	r=0.610	1321:1327	The concentration of butyric acid decreased significantly in bladder cancer patients, and the quantities of fecal butyric acid were significantly and positively associated with fruit intake (r=0.610, p<0.01).
32674247	11	40	theme	acid	1252:1255	arg1	quantities					1224:1233	the quantities	1220:1233	the quantities of fecal butyric acid	1220:1255	The concentration of butyric acid decreased significantly in bladder cancer patients, and the quantities of fecal butyric acid were significantly and positively associated with fruit intake (r=0.610, p<0.01).
32674247	0	41	theme	cancer	45:50	arg1	patients					52:59	bladder cancer patients	37:59	bladder cancer patients	37:59	Gut microbial composition changes in bladder cancer patients: A case-control study in Harbin, China.
32674247	7	42	theme	enzyme-linked	748:760	arg1	kits					782:785	enzyme-linked immunosorbent assay kits	748:785	enzyme-linked immunosorbent assay kits	748:785	The concentrations of lipopolysaccharide and D-lactic acid in serum were determined by enzyme-linked immunosorbent assay kits.
32674247	11	43	theme	fecal	1238:1242	arg1	acid					1252:1255	fecal butyric acid	1238:1255	fecal butyric acid	1238:1255	The concentration of butyric acid decreased significantly in bladder cancer patients, and the quantities of fecal butyric acid were significantly and positively associated with fruit intake (r=0.610, p<0.01).
32674247	1	44	theme	cancer	194:199	arg1	patients					201:208	bladder cancer patients	186:208	bladder cancer patients	186:208	BACKGROUND AND OBJECTIVES This study aimed to explore the changes of gut bacteria in bladder cancer patients.
32674247	11	45	theme	butyric	1151:1157	arg1	acid					1159:1162	butyric acid	1151:1162	butyric acid	1151:1162	The concentration of butyric acid decreased significantly in bladder cancer patients, and the quantities of fecal butyric acid were significantly and positively associated with fruit intake (r=0.610, p<0.01).
32674247	10	46	theme	Prevotella	985:994	arg1	numbers					954:960	The numbers	950:960	The numbers of domain bacteria and Prevotella	950:994	The numbers of domain bacteria and Prevotella were significantly and positively associated with fruit intake (r=0.002, p<0.05 for domain bacteria; r=0.004, p<0.05 for Prevotella).
32674247	9	47	theme	cancer	923:928	arg1	patients					930:937	bladder cancer patients	915:937	bladder cancer patients	915:937	The numbers of Clostridium cluster XI and Prevotella in bladder cancer patients decreased.
32674247	9	48	theme	cluster	886:892	arg1	XI					894:895	Clostridium cluster XI	874:895	Clostridium cluster XI	874:895	The numbers of Clostridium cluster XI and Prevotella in bladder cancer patients decreased.
32674247	7	49	theme	acid	715:718	arg1	concentrations					665:678	The concentrations	661:678	The concentrations of lipopolysaccharide and D-lactic acid in serum	661:727	The concentrations of lipopolysaccharide and D-lactic acid in serum were determined by enzyme-linked immunosorbent assay kits.
32674247	12	50	theme	bladder	1458:1464	arg1	patients					1473:1480	bladder cancer patients	1458:1480	bladder cancer patients	1458:1480	The concentrations of lipopolysaccharide and D-lactic acid, two sensitive markers of gut permeability, were greater in bladder cancer patients.
32674247	4	51	theme	total	391:395	arg1	DNA					405:407	the total genomic DNA	387:407	the total genomic DNA extracted from human feces	387:434	Pyrosequencing of the total genomic DNA extracted from human feces was carried out by Illumina HiSeq 2000.
32674247	12	52	theme	gut	1424:1426	arg1	permeability					1428:1439	gut permeability	1424:1439	gut permeability	1424:1439	The concentrations of lipopolysaccharide and D-lactic acid, two sensitive markers of gut permeability, were greater in bladder cancer patients.
32674247	4	53	theme	DNA	405:407	arg1	Pyrosequencing					369:382	Pyrosequencing	369:382	Pyrosequencing of the total genomic DNA extracted from human feces	369:434	Pyrosequencing of the total genomic DNA extracted from human feces was carried out by Illumina HiSeq 2000.
32674247	9	54	from	numbers	863:869	arg1	patients					930:937	bladder cancer patients	915:937	bladder cancer patients	915:937	The numbers of Clostridium cluster XI and Prevotella in bladder cancer patients decreased.
32674247	2	55	theme	bladder	252:258	arg1	patients					267:274	Newly diagnosed bladder cancer patients	236:274	Newly diagnosed bladder cancer patients	236:274	METHODS AND STUDY DESIGN Newly diagnosed bladder cancer patients were recruited.
32674247	5	56	theme	copy	480:483	arg1	number					485:490	The copy number	476:490	The copy number of target DNA for bacteria	476:517	The copy number of target DNA for bacteria was determined by real-time quantitative PCR assay.
32674247	12	57	theme	sensitive	1403:1411	arg1	acid					1393:1396	D-lactic acid	1384:1396	D-lactic acid	1384:1396	The concentrations of lipopolysaccharide and D-lactic acid, two sensitive markers of gut permeability, were greater in bladder cancer patients.
32674247	12	57	theme	sensitive	1403:1411	arg1	markers					1413:1419	two sensitive markers	1399:1419	two sensitive markers of gut permeability	1399:1439	The concentrations of lipopolysaccharide and D-lactic acid, two sensitive markers of gut permeability, were greater in bladder cancer patients.
32674247	6	58	theme	fatty	589:593	arg1	acids					595:599	Fecal short chain fatty acids	571:599	Fecal short chain fatty acids contents	571:608	Fecal short chain fatty acids contents were measured by gas chromatography (GC) analysis.
32674247	13	59	theme	structural	1586:1595	arg1	CONCLUSIONS					1483:1493	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity	1483:1605	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity	1483:1605	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity were found in bladder cancer patients, which might be associated with inadequate fruit intake.
32674247	13	59	theme	structural	1586:1595	arg1	integrity					1597:1605	impaired intestinal structural integrity	1566:1605	impaired intestinal structural integrity	1566:1605	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity were found in bladder cancer patients, which might be associated with inadequate fruit intake.
32674247	12	60	theme	lipopolysaccharide	1361:1378	arg1	greater					1447:1453	greater	1447:1453	greater	1447:1453	The concentrations of lipopolysaccharide and D-lactic acid, two sensitive markers of gut permeability, were greater in bladder cancer patients.
32674247	12	60	theme	lipopolysaccharide	1361:1378	arg1	concentrations					1343:1356	The concentrations	1339:1356	The concentrations of lipopolysaccharide and D-lactic acid, two sensitive markers of gut permeability,	1339:1440	The concentrations of lipopolysaccharide and D-lactic acid, two sensitive markers of gut permeability, were greater in bladder cancer patients.
32674247	6	61	theme	short	577:581	arg1	acids					595:599	Fecal short chain fatty acids	571:599	Fecal short chain fatty acids contents	571:608	Fecal short chain fatty acids contents were measured by gas chromatography (GC) analysis.
32674247	1	62	theme	gut	170:172	arg1	bacteria					174:181	gut bacteria	170:181	gut bacteria	170:181	BACKGROUND AND OBJECTIVES This study aimed to explore the changes of gut bacteria in bladder cancer patients.
32674247	0	63	from	study	77:81	arg1	Harbin					86:91	Harbin	86:91	Harbin	86:91	Gut microbial composition changes in bladder cancer patients: A case-control study in Harbin, China.
32674247	0	63	from	study	77:81	arg1	China					94:98	China	94:98	China	94:98	Gut microbial composition changes in bladder cancer patients: A case-control study in Harbin, China.
32674247	12	64	theme	D-lactic	1384:1391	arg1	acid					1393:1396	D-lactic acid	1384:1396	D-lactic acid	1384:1396	The concentrations of lipopolysaccharide and D-lactic acid, two sensitive markers of gut permeability, were greater in bladder cancer patients.
32674247	12	64	theme	D-lactic	1384:1391	arg1	markers					1413:1419	two sensitive markers	1399:1419	two sensitive markers of gut permeability	1399:1439	The concentrations of lipopolysaccharide and D-lactic acid, two sensitive markers of gut permeability, were greater in bladder cancer patients.
32674247	7	65	theme	lipopolysaccharide	683:700	arg1	concentrations					665:678	The concentrations	661:678	The concentrations of lipopolysaccharide and D-lactic acid in serum	661:727	The concentrations of lipopolysaccharide and D-lactic acid in serum were determined by enzyme-linked immunosorbent assay kits.
32674247	13	66	theme	impaired	1566:1573	arg1	CONCLUSIONS					1483:1493	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity	1483:1605	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity	1483:1605	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity were found in bladder cancer patients, which might be associated with inadequate fruit intake.
32674247	13	66	theme	impaired	1566:1573	arg1	integrity					1597:1605	impaired intestinal structural integrity	1566:1605	impaired intestinal structural integrity	1566:1605	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity were found in bladder cancer patients, which might be associated with inadequate fruit intake.
32674247	5	67	theme	real-time	537:545	arg1	assay					564:568	real-time quantitative PCR assay	537:568	real-time quantitative PCR assay	537:568	The copy number of target DNA for bacteria was determined by real-time quantitative PCR assay.
32674247	13	68	dep	CONCLUSIONS	1483:1493	arg1	CONCLUSIONS					1483:1493	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity	1483:1605	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity	1483:1605	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity were found in bladder cancer patients, which might be associated with inadequate fruit intake.
32674247	13	68	dep	CONCLUSIONS	1483:1493	arg1	concentrations					1547:1560	decreased butyric acid concentrations	1524:1560	decreased butyric acid concentrations	1524:1560	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity were found in bladder cancer patients, which might be associated with inadequate fruit intake.
32674247	13	68	dep	CONCLUSIONS	1483:1493	arg1	Dysbiosis					1495:1503	Dysbiosis	1495:1503	Dysbiosis of gut microbiota	1495:1521	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity were found in bladder cancer patients, which might be associated with inadequate fruit intake.
32674247	13	68	dep	CONCLUSIONS	1483:1493	arg1	integrity					1597:1605	impaired intestinal structural integrity	1566:1605	impaired intestinal structural integrity	1566:1605	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity were found in bladder cancer patients, which might be associated with inadequate fruit intake.
32674247	6	69	theme	gas	627:629	arg1	GC					647:648	GC	647:648	GC	647:648	Fecal short chain fatty acids contents were measured by gas chromatography (GC) analysis.
32674247	6	69	theme	gas	627:629	arg1	chromatography					631:644	gas chromatography	627:644	gas chromatography (GC) analysis	627:658	Fecal short chain fatty acids contents were measured by gas chromatography (GC) analysis.
32674247	2	70	theme	STUDY	223:227	arg1	DESIGN					229:234	STUDY DESIGN	223:234	STUDY DESIGN	223:234	METHODS AND STUDY DESIGN Newly diagnosed bladder cancer patients were recruited.
32674247	5	71	theme	DNA	502:504	arg1	number					485:490	The copy number	476:490	The copy number of target DNA for bacteria	476:517	The copy number of target DNA for bacteria was determined by real-time quantitative PCR assay.
32674247	0	72	from	changes	26:32	arg1	patients					52:59	bladder cancer patients	37:59	bladder cancer patients	37:59	Gut microbial composition changes in bladder cancer patients: A case-control study in Harbin, China.
32674247	10	73	theme	domain	1080:1085	arg1	bacteria					1087:1094	domain bacteria	1080:1094	domain bacteria	1080:1094	The numbers of domain bacteria and Prevotella were significantly and positively associated with fruit intake (r=0.002, p<0.05 for domain bacteria; r=0.004, p<0.05 for Prevotella).
32674247	11	74	theme	butyric	1244:1250	arg1	acid					1252:1255	fecal butyric acid	1238:1255	fecal butyric acid	1238:1255	The concentration of butyric acid decreased significantly in bladder cancer patients, and the quantities of fecal butyric acid were significantly and positively associated with fruit intake (r=0.610, p<0.01).
32674247	7	75	from	concentrations	665:678	arg1	serum					723:727	serum	723:727	serum	723:727	The concentrations of lipopolysaccharide and D-lactic acid in serum were determined by enzyme-linked immunosorbent assay kits.
32674247	8	76	theme	Fruit	796:800	arg1	intake					802:807	RESULTS Fruit intake	788:807	RESULTS Fruit intake	788:807	RESULTS Fruit intake was significantly lower than in healthy controls.
32674247	11	77	theme	acid	1159:1162	arg1	concentration					1134:1146	The concentration	1130:1146	The concentration of butyric acid	1130:1162	The concentration of butyric acid decreased significantly in bladder cancer patients, and the quantities of fecal butyric acid were significantly and positively associated with fruit intake (r=0.610, p<0.01).
32674247	3	78	theme	personal	341:348	arg1	behavior					350:357	personal behavior	341:357	personal behavior	341:357	All participants completed a questionnaire about personal behavior and diet.
32674247	1	79	dep	BACKGROUND	101:110	arg1	aimed					138:142	aimed	138:142	aimed to explore the changes of gut bacteria in bladder cancer patients	138:208	BACKGROUND AND OBJECTIVES This study aimed to explore the changes of gut bacteria in bladder cancer patients.
32674247	0	80	theme	microbial	4:12	arg1	changes					26:32	Gut microbial composition changes	0:32	Gut microbial composition changes in bladder cancer patients: A case-control study in Harbin, China.	0:99	Gut microbial composition changes in bladder cancer patients: A case-control study in Harbin, China.
32674247	5	81	theme	PCR	560:562	arg1	assay					564:568	real-time quantitative PCR assay	537:568	real-time quantitative PCR assay	537:568	The copy number of target DNA for bacteria was determined by real-time quantitative PCR assay.
32674247	11	82	theme	fruit	1307:1311	arg1	intake					1313:1318	fruit intake	1307:1318	fruit intake (r=0.610, p<0.01)	1307:1336	The concentration of butyric acid decreased significantly in bladder cancer patients, and the quantities of fecal butyric acid were significantly and positively associated with fruit intake (r=0.610, p<0.01).
32674247	13	83	theme	microbiota	1512:1521	arg1	CONCLUSIONS					1483:1493	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity	1483:1605	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity	1483:1605	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity were found in bladder cancer patients, which might be associated with inadequate fruit intake.
32674247	13	83	theme	microbiota	1512:1521	arg1	integrity					1597:1605	impaired intestinal structural integrity	1566:1605	impaired intestinal structural integrity	1566:1605	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity were found in bladder cancer patients, which might be associated with inadequate fruit intake.
32674247	13	83	theme	microbiota	1512:1521	arg1	Dysbiosis					1495:1503	Dysbiosis	1495:1503	Dysbiosis of gut microbiota	1495:1521	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity were found in bladder cancer patients, which might be associated with inadequate fruit intake.
32674247	13	83	theme	microbiota	1512:1521	arg1	concentrations					1547:1560	decreased butyric acid concentrations	1524:1560	decreased butyric acid concentrations	1524:1560	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity were found in bladder cancer patients, which might be associated with inadequate fruit intake.
32674247	11	84	theme	bladder	1191:1197	arg1	patients					1206:1213	bladder cancer patients	1191:1213	bladder cancer patients	1191:1213	The concentration of butyric acid decreased significantly in bladder cancer patients, and the quantities of fecal butyric acid were significantly and positively associated with fruit intake (r=0.610, p<0.01).
32674247	0	85	theme	bladder	37:43	arg1	patients					52:59	bladder cancer patients	37:59	bladder cancer patients	37:59	Gut microbial composition changes in bladder cancer patients: A case-control study in Harbin, China.
32674247	9	86	theme	Clostridium	874:884	arg1	XI					894:895	Clostridium cluster XI	874:895	Clostridium cluster XI	874:895	The numbers of Clostridium cluster XI and Prevotella in bladder cancer patients decreased.
32674247	10	87	theme	domain	965:970	arg1	bacteria					972:979	domain bacteria	965:979	domain bacteria	965:979	The numbers of domain bacteria and Prevotella were significantly and positively associated with fruit intake (r=0.002, p<0.05 for domain bacteria; r=0.004, p<0.05 for Prevotella).
32674247	9	88	theme	XI	894:895	arg1	numbers					863:869	The numbers	859:869	The numbers of Clostridium cluster XI and Prevotella in bladder cancer patients	859:937	The numbers of Clostridium cluster XI and Prevotella in bladder cancer patients decreased.
32674247	12	89	theme	cancer	1466:1471	arg1	patients					1473:1480	bladder cancer patients	1458:1480	bladder cancer patients	1458:1480	The concentrations of lipopolysaccharide and D-lactic acid, two sensitive markers of gut permeability, were greater in bladder cancer patients.
32674247	0	90	dep	changes	26:32	arg1	study					77:81	A case-control study	62:81	Gut microbial composition changes in bladder cancer patients: A case-control study in Harbin, China.	0:99	Gut microbial composition changes in bladder cancer patients: A case-control study in Harbin, China.
32674247	9	91	theme	Prevotella	901:910	arg1	numbers					863:869	The numbers	859:869	The numbers of Clostridium cluster XI and Prevotella in bladder cancer patients	859:937	The numbers of Clostridium cluster XI and Prevotella in bladder cancer patients decreased.
32674247	13	92	theme	fruit	1688:1692	arg1	intake					1694:1699	inadequate fruit intake	1677:1699	inadequate fruit intake	1677:1699	CONCLUSIONS Dysbiosis of gut microbiota, decreased butyric acid concentrations and impaired intestinal structural integrity were found in bladder cancer patients, which might be associated with inadequate fruit intake.
32674247	9	93	theme	bladder	915:921	arg1	patients					930:937	bladder cancer patients	915:937	bladder cancer patients	915:937	The numbers of Clostridium cluster XI and Prevotella in bladder cancer patients decreased.
32885950	1	0	contain	has	149:151	arg2	mechanisms					161:170	its own mechanisms	153:170	its own mechanisms	153:170	A pathogenic bacterium has its own mechanisms for not only pathogenic attack but also exogenous invasion defense, in which the bacterial cell wall is the front line of attack and defense.
32885950	1	0	contain	has	149:151	arg1	bacterium					139:147	A pathogenic bacterium	126:147	A pathogenic bacterium	126:147	A pathogenic bacterium has its own mechanisms for not only pathogenic attack but also exogenous invasion defense, in which the bacterial cell wall is the front line of attack and defense.
32885950	5	1	theme	complex	1213:1219	arg1	procedures					1248:1257	any tedious and complex "cell-broken" pretreatment procedures	1197:1257	any tedious and complex "cell-broken" pretreatment procedures that might induce racemization of the d-X	1197:1299	This approach allows direct quantification of the d-X in situ in the cell wall using 158Gd, 153Eu, and 154Sm species-unspecific isotope dilution inductively coupled plasma mass spectrometry, avoiding any tedious and complex "cell-broken" pretreatment procedures that might induce racemization of the d-X.
32885950	6	2	theme	composition-dependent	1602:1622	arg1	mechanisms					1656:1665	the structure- and composition-dependent bacterial antibiotic resistance mechanisms	1583:1665	the structure- and composition-dependent bacterial antibiotic resistance mechanisms	1583:1665	The obtained site-specific and accurate in situ information about the d-X enables quantitative monitoring of the bacterial response when Staphylococcus aureus meets vancomycin, showing that the amounts of azide-d-Leu and ketone-d-Met assembled are more important after determining the structure- and composition-dependent bacterial antibiotic resistance mechanisms.
32885950	1	3	theme	bacterial	253:261	arg1	line					286:289	the front line	276:289	the front line of attack and defense	276:311	A pathogenic bacterium has its own mechanisms for not only pathogenic attack but also exogenous invasion defense, in which the bacterial cell wall is the front line of attack and defense.
32885950	1	3	theme	bacterial	253:261	arg1	wall					268:271	the bacterial cell wall	249:271	the bacterial cell wall	249:271	A pathogenic bacterium has its own mechanisms for not only pathogenic attack but also exogenous invasion defense, in which the bacterial cell wall is the front line of attack and defense.
32885950	0	4	theme	Response	92:99	arg1	Monitoring					64:73	Quantitative Monitoring	51:73	Quantitative Monitoring of the Bacterial Response to Vancomycin Treatment	51:123	A Biochemical Lanthanide-Encoding Approach Enables Quantitative Monitoring of the Bacterial Response to Vancomycin Treatment.
32885950	3	5	theme	chromatography-based	735:754	arg1	methods					756:762	traditional high-performance liquid chromatography-based methods	699:762	traditional high-performance liquid chromatography-based methods	699:762	This approach overcomes the difficulties regarding quantification and accuracy issues encountered by the popular optical imaging and traditional high-performance liquid chromatography-based methods.
32885950	5	6	theme	coupled	1154:1160	arg1	spectrometry					1174:1185	inductively coupled plasma mass spectrometry	1142:1185	inductively coupled plasma mass spectrometry	1142:1185	This approach allows direct quantification of the d-X in situ in the cell wall using 158Gd, 153Eu, and 154Sm species-unspecific isotope dilution inductively coupled plasma mass spectrometry, avoiding any tedious and complex "cell-broken" pretreatment procedures that might induce racemization of the d-X.
32885950	2	7	theme	lanthanide-encoding	341:359	arg1	approach					361:368	a biochemical lanthanide-encoding approach	327:368	a biochemical lanthanide-encoding approach to quantify the uncanonical d-amino acid (d-X) that was edited in a small proportion into the terminal acyl-d-Ala-d-X of nascent peptidoglycan UDP-MurNAc-pentapeptides in the bacterial cell wall	327:563	We developed a biochemical lanthanide-encoding approach to quantify the uncanonical d-amino acid (d-X) that was edited in a small proportion into the terminal acyl-d-Ala-d-X of nascent peptidoglycan UDP-MurNAc-pentapeptides in the bacterial cell wall.
32885950	1	8	theme	cell	263:266	arg1	line					286:289	the front line	276:289	the front line of attack and defense	276:311	A pathogenic bacterium has its own mechanisms for not only pathogenic attack but also exogenous invasion defense, in which the bacterial cell wall is the front line of attack and defense.
32885950	1	8	theme	cell	263:266	arg1	wall					268:271	the bacterial cell wall	249:271	the bacterial cell wall	249:271	A pathogenic bacterium has its own mechanisms for not only pathogenic attack but also exogenous invasion defense, in which the bacterial cell wall is the front line of attack and defense.
32885950	5	9	theme	pretreatment	1235:1246	arg1	procedures					1248:1257	any tedious and complex "cell-broken" pretreatment procedures	1197:1257	any tedious and complex "cell-broken" pretreatment procedures that might induce racemization of the d-X	1197:1299	This approach allows direct quantification of the d-X in situ in the cell wall using 158Gd, 153Eu, and 154Sm species-unspecific isotope dilution inductively coupled plasma mass spectrometry, avoiding any tedious and complex "cell-broken" pretreatment procedures that might induce racemization of the d-X.
32885950	3	10	theme	optical	679:685	arg1	imaging					687:693	the popular optical imaging	667:693	the popular optical imaging	667:693	This approach overcomes the difficulties regarding quantification and accuracy issues encountered by the popular optical imaging and traditional high-performance liquid chromatography-based methods.
32885950	4	11	used	used	817:820	arg2	ketone-d-Met					799:810	ketone-d-Met	799:810	ketone-d-Met	799:810	Newly synthesized azide-d-Leu and ketone-d-Met were used together with alkynyl-d-Ala for their metabolic assembly and then bioorthogonally encoded by the correspondingly fabricated DBCO-DOTA-Gd, HNO-DOTA-Eu, and azide-DOTA-Sm tags.
32885950	4	11	used	used	817:820	arg2	azide-d-Leu					783:793	Newly synthesized azide-d-Leu	765:793	Newly synthesized azide-d-Leu	765:793	Newly synthesized azide-d-Leu and ketone-d-Met were used together with alkynyl-d-Ala for their metabolic assembly and then bioorthogonally encoded by the correspondingly fabricated DBCO-DOTA-Gd, HNO-DOTA-Eu, and azide-DOTA-Sm tags.
32885950	0	12	theme	Vancomycin	104:113	arg1	Treatment					115:123	Vancomycin Treatment	104:123	Vancomycin Treatment	104:123	A Biochemical Lanthanide-Encoding Approach Enables Quantitative Monitoring of the Bacterial Response to Vancomycin Treatment.
32885950	4	13	theme	azide-DOTA-Sm	977:989	arg1	tags					991:994	azide-DOTA-Sm tags	977:994	azide-DOTA-Sm tags	977:994	Newly synthesized azide-d-Leu and ketone-d-Met were used together with alkynyl-d-Ala for their metabolic assembly and then bioorthogonally encoded by the correspondingly fabricated DBCO-DOTA-Gd, HNO-DOTA-Eu, and azide-DOTA-Sm tags.
32885950	5	14	theme	cell	1066:1069	arg1	wall					1071:1074	the cell wall	1062:1074	the cell wall using 158Gd, 153Eu, and 154Sm species-unspecific isotope dilution inductively coupled plasma mass spectrometry	1062:1185	This approach allows direct quantification of the d-X in situ in the cell wall using 158Gd, 153Eu, and 154Sm species-unspecific isotope dilution inductively coupled plasma mass spectrometry, avoiding any tedious and complex "cell-broken" pretreatment procedures that might induce racemization of the d-X.
32885950	7	15	theme	intermediate-resistant	1828:1849	arg1	vancomycin					1817:1826	vancomycin	1817:1826	vancomycin intermediate-resistant S. aureus	1817:1859	In addition, we found that the combined use of vancomycin and d-Ala restores the efficacy of vancomycin and might be a wise and simple way to combat vancomycin intermediate-resistant S. aureus.
32885950	2	16	theme	UDP-MurNAc-pentapeptides	513:536	arg1	acyl-d-Ala-d-X					473:486	the terminal acyl-d-Ala-d-X	460:486	the terminal acyl-d-Ala-d-X of nascent peptidoglycan UDP-MurNAc-pentapeptides in the bacterial cell wall	460:563	We developed a biochemical lanthanide-encoding approach to quantify the uncanonical d-amino acid (d-X) that was edited in a small proportion into the terminal acyl-d-Ala-d-X of nascent peptidoglycan UDP-MurNAc-pentapeptides in the bacterial cell wall.
32885950	2	17	theme	biochemical	329:339	arg1	approach					361:368	a biochemical lanthanide-encoding approach	327:368	a biochemical lanthanide-encoding approach to quantify the uncanonical d-amino acid (d-X) that was edited in a small proportion into the terminal acyl-d-Ala-d-X of nascent peptidoglycan UDP-MurNAc-pentapeptides in the bacterial cell wall	327:563	We developed a biochemical lanthanide-encoding approach to quantify the uncanonical d-amino acid (d-X) that was edited in a small proportion into the terminal acyl-d-Ala-d-X of nascent peptidoglycan UDP-MurNAc-pentapeptides in the bacterial cell wall.
32885950	1	18	from	line	286:289	arg1	mechanisms					161:170	its own mechanisms	153:170	its own mechanisms	153:170	A pathogenic bacterium has its own mechanisms for not only pathogenic attack but also exogenous invasion defense, in which the bacterial cell wall is the front line of attack and defense.
32885950	4	19	theme	metabolic	860:868	arg1	assembly					870:877	their metabolic assembly	854:877	their metabolic assembly	854:877	Newly synthesized azide-d-Leu and ketone-d-Met were used together with alkynyl-d-Ala for their metabolic assembly and then bioorthogonally encoded by the correspondingly fabricated DBCO-DOTA-Gd, HNO-DOTA-Eu, and azide-DOTA-Sm tags.
32885950	2	20	theme	peptidoglycan	499:511	arg1	UDP-MurNAc-pentapeptides					513:536	nascent peptidoglycan UDP-MurNAc-pentapeptides	491:536	nascent peptidoglycan UDP-MurNAc-pentapeptides	491:536	We developed a biochemical lanthanide-encoding approach to quantify the uncanonical d-amino acid (d-X) that was edited in a small proportion into the terminal acyl-d-Ala-d-X of nascent peptidoglycan UDP-MurNAc-pentapeptides in the bacterial cell wall.
32885950	4	21	theme	fabricated	935:944	arg1	DBCO-DOTA-Gd					946:957	the correspondingly fabricated DBCO-DOTA-Gd	915:957	the correspondingly fabricated DBCO-DOTA-Gd	915:957	Newly synthesized azide-d-Leu and ketone-d-Met were used together with alkynyl-d-Ala for their metabolic assembly and then bioorthogonally encoded by the correspondingly fabricated DBCO-DOTA-Gd, HNO-DOTA-Eu, and azide-DOTA-Sm tags.
32885950	7	22	theme	vancomycin	1715:1724	arg1	use					1708:1710	the combined use	1695:1710	the combined use of vancomycin and d-Ala	1695:1734	In addition, we found that the combined use of vancomycin and d-Ala restores the efficacy of vancomycin and might be a wise and simple way to combat vancomycin intermediate-resistant S. aureus.
32885950	1	23	theme	pathogenic	185:194	arg1	attack					196:201	pathogenic attack	185:201	not only pathogenic attack but also exogenous invasion defense	176:237	A pathogenic bacterium has its own mechanisms for not only pathogenic attack but also exogenous invasion defense, in which the bacterial cell wall is the front line of attack and defense.
32885950	0	24	theme	Biochemical	2:12	arg1	Approach					34:41	A Biochemical Lanthanide-Encoding Approach	0:41	A Biochemical Lanthanide-Encoding Approach	0:41	A Biochemical Lanthanide-Encoding Approach Enables Quantitative Monitoring of the Bacterial Response to Vancomycin Treatment.
32885950	2	25	theme	terminal	464:471	arg1	acyl-d-Ala-d-X					473:486	the terminal acyl-d-Ala-d-X	460:486	the terminal acyl-d-Ala-d-X of nascent peptidoglycan UDP-MurNAc-pentapeptides in the bacterial cell wall	460:563	We developed a biochemical lanthanide-encoding approach to quantify the uncanonical d-amino acid (d-X) that was edited in a small proportion into the terminal acyl-d-Ala-d-X of nascent peptidoglycan UDP-MurNAc-pentapeptides in the bacterial cell wall.
32885950	2	26	theme	nascent	491:497	arg1	UDP-MurNAc-pentapeptides					513:536	nascent peptidoglycan UDP-MurNAc-pentapeptides	491:536	nascent peptidoglycan UDP-MurNAc-pentapeptides	491:536	We developed a biochemical lanthanide-encoding approach to quantify the uncanonical d-amino acid (d-X) that was edited in a small proportion into the terminal acyl-d-Ala-d-X of nascent peptidoglycan UDP-MurNAc-pentapeptides in the bacterial cell wall.
32885950	5	27	theme	d-X	1047:1049	arg1	quantification					1025:1038	direct quantification	1018:1038	direct quantification of the d-X	1018:1049	This approach allows direct quantification of the d-X in situ in the cell wall using 158Gd, 153Eu, and 154Sm species-unspecific isotope dilution inductively coupled plasma mass spectrometry, avoiding any tedious and complex "cell-broken" pretreatment procedures that might induce racemization of the d-X.
32885950	5	28	theme	tedious	1201:1207	arg1	procedures					1248:1257	any tedious and complex "cell-broken" pretreatment procedures	1197:1257	any tedious and complex "cell-broken" pretreatment procedures that might induce racemization of the d-X	1197:1299	This approach allows direct quantification of the d-X in situ in the cell wall using 158Gd, 153Eu, and 154Sm species-unspecific isotope dilution inductively coupled plasma mass spectrometry, avoiding any tedious and complex "cell-broken" pretreatment procedures that might induce racemization of the d-X.
32885950	2	29	theme	uncanonical	386:396	arg1	d-X					412:414	d-X	412:414	d-X	412:414	We developed a biochemical lanthanide-encoding approach to quantify the uncanonical d-amino acid (d-X) that was edited in a small proportion into the terminal acyl-d-Ala-d-X of nascent peptidoglycan UDP-MurNAc-pentapeptides in the bacterial cell wall.
32885950	2	29	theme	uncanonical	386:396	arg1	acid					406:409	the uncanonical d-amino acid	382:409	the uncanonical d-amino acid (d-X) that was edited in a small proportion into the terminal acyl-d-Ala-d-X of nascent peptidoglycan UDP-MurNAc-pentapeptides in the bacterial cell wall	382:563	We developed a biochemical lanthanide-encoding approach to quantify the uncanonical d-amino acid (d-X) that was edited in a small proportion into the terminal acyl-d-Ala-d-X of nascent peptidoglycan UDP-MurNAc-pentapeptides in the bacterial cell wall.
32885950	5	30	theme	"	1233:1233	arg1	procedures					1248:1257	any tedious and complex "cell-broken" pretreatment procedures	1197:1257	any tedious and complex "cell-broken" pretreatment procedures that might induce racemization of the d-X	1197:1299	This approach allows direct quantification of the d-X in situ in the cell wall using 158Gd, 153Eu, and 154Sm species-unspecific isotope dilution inductively coupled plasma mass spectrometry, avoiding any tedious and complex "cell-broken" pretreatment procedures that might induce racemization of the d-X.
32885950	7	31	theme	wise	1787:1790	arg1	way					1803:1805	a wise and simple way	1785:1805	a wise and simple way to combat vancomycin intermediate-resistant S. aureus	1785:1859	In addition, we found that the combined use of vancomycin and d-Ala restores the efficacy of vancomycin and might be a wise and simple way to combat vancomycin intermediate-resistant S. aureus.
32885950	5	32	theme	plasma	1162:1167	arg1	spectrometry					1174:1185	inductively coupled plasma mass spectrometry	1142:1185	inductively coupled plasma mass spectrometry	1142:1185	This approach allows direct quantification of the d-X in situ in the cell wall using 158Gd, 153Eu, and 154Sm species-unspecific isotope dilution inductively coupled plasma mass spectrometry, avoiding any tedious and complex "cell-broken" pretreatment procedures that might induce racemization of the d-X.
32885950	7	33	theme	combined	1699:1706	arg1	use					1708:1710	the combined use	1695:1710	the combined use of vancomycin and d-Ala	1695:1734	In addition, we found that the combined use of vancomycin and d-Ala restores the efficacy of vancomycin and might be a wise and simple way to combat vancomycin intermediate-resistant S. aureus.
32885950	1	34	theme	front	280:284	arg1	line					286:289	the front line	276:289	the front line of attack and defense	276:311	A pathogenic bacterium has its own mechanisms for not only pathogenic attack but also exogenous invasion defense, in which the bacterial cell wall is the front line of attack and defense.
32885950	1	34	theme	front	280:284	arg1	wall					268:271	the bacterial cell wall	249:271	the bacterial cell wall	249:271	A pathogenic bacterium has its own mechanisms for not only pathogenic attack but also exogenous invasion defense, in which the bacterial cell wall is the front line of attack and defense.
32885950	0	35	theme	Lanthanide-Encoding	14:32	arg1	Approach					34:41	A Biochemical Lanthanide-Encoding Approach	0:41	A Biochemical Lanthanide-Encoding Approach	0:41	A Biochemical Lanthanide-Encoding Approach Enables Quantitative Monitoring of the Bacterial Response to Vancomycin Treatment.
32885950	5	36	theme	cell-broken	1222:1232	arg1	procedures					1248:1257	any tedious and complex "cell-broken" pretreatment procedures	1197:1257	any tedious and complex "cell-broken" pretreatment procedures that might induce racemization of the d-X	1197:1299	This approach allows direct quantification of the d-X in situ in the cell wall using 158Gd, 153Eu, and 154Sm species-unspecific isotope dilution inductively coupled plasma mass spectrometry, avoiding any tedious and complex "cell-broken" pretreatment procedures that might induce racemization of the d-X.
32885950	6	37	theme	ketone-d-Met	1523:1534	arg1	ketone-d-Met					1523:1534	ketone-d-Met	1523:1534	ketone-d-Met	1523:1534	The obtained site-specific and accurate in situ information about the d-X enables quantitative monitoring of the bacterial response when Staphylococcus aureus meets vancomycin, showing that the amounts of azide-d-Leu and ketone-d-Met assembled are more important after determining the structure- and composition-dependent bacterial antibiotic resistance mechanisms.
32885950	6	37	theme	ketone-d-Met	1523:1534	arg1	azide-d-Leu					1507:1517	azide-d-Leu	1507:1517	azide-d-Leu	1507:1517	The obtained site-specific and accurate in situ information about the d-X enables quantitative monitoring of the bacterial response when Staphylococcus aureus meets vancomycin, showing that the amounts of azide-d-Leu and ketone-d-Met assembled are more important after determining the structure- and composition-dependent bacterial antibiotic resistance mechanisms.
32885950	6	37	theme	ketone-d-Met	1523:1534	arg1	important					1555:1563	important	1555:1563	important	1555:1563	The obtained site-specific and accurate in situ information about the d-X enables quantitative monitoring of the bacterial response when Staphylococcus aureus meets vancomycin, showing that the amounts of azide-d-Leu and ketone-d-Met assembled are more important after determining the structure- and composition-dependent bacterial antibiotic resistance mechanisms.
32885950	6	37	theme	ketone-d-Met	1523:1534	arg1	amounts					1496:1502	the amounts	1492:1502	the amounts of azide-d-Leu and ketone-d-Met assembled	1492:1544	The obtained site-specific and accurate in situ information about the d-X enables quantitative monitoring of the bacterial response when Staphylococcus aureus meets vancomycin, showing that the amounts of azide-d-Leu and ketone-d-Met assembled are more important after determining the structure- and composition-dependent bacterial antibiotic resistance mechanisms.
32885950	2	38	from	acyl-d-Ala-d-X	473:486	arg1	wall					560:563	the bacterial cell wall	541:563	the bacterial cell wall	541:563	We developed a biochemical lanthanide-encoding approach to quantify the uncanonical d-amino acid (d-X) that was edited in a small proportion into the terminal acyl-d-Ala-d-X of nascent peptidoglycan UDP-MurNAc-pentapeptides in the bacterial cell wall.
32885950	6	39	dep	in	1342:1343	arg1	situ					1345:1348	situ	1345:1348	situ	1345:1348	The obtained site-specific and accurate in situ information about the d-X enables quantitative monitoring of the bacterial response when Staphylococcus aureus meets vancomycin, showing that the amounts of azide-d-Leu and ketone-d-Met assembled are more important after determining the structure- and composition-dependent bacterial antibiotic resistance mechanisms.
32885950	7	40	theme	simple	1796:1801	arg1	way					1803:1805	a wise and simple way	1785:1805	a wise and simple way to combat vancomycin intermediate-resistant S. aureus	1785:1859	In addition, we found that the combined use of vancomycin and d-Ala restores the efficacy of vancomycin and might be a wise and simple way to combat vancomycin intermediate-resistant S. aureus.
32885950	1	41	theme	exogenous	212:220	arg1	defense					231:237	exogenous invasion defense	212:237	not only pathogenic attack but also exogenous invasion defense	176:237	A pathogenic bacterium has its own mechanisms for not only pathogenic attack but also exogenous invasion defense, in which the bacterial cell wall is the front line of attack and defense.
32885950	0	42	theme	Quantitative	51:62	arg1	Monitoring					64:73	Quantitative Monitoring	51:73	Quantitative Monitoring of the Bacterial Response to Vancomycin Treatment	51:123	A Biochemical Lanthanide-Encoding Approach Enables Quantitative Monitoring of the Bacterial Response to Vancomycin Treatment.
32885950	5	43	theme	d-X	1297:1299	arg1	racemization					1277:1288	racemization	1277:1288	racemization of the d-X	1277:1299	This approach allows direct quantification of the d-X in situ in the cell wall using 158Gd, 153Eu, and 154Sm species-unspecific isotope dilution inductively coupled plasma mass spectrometry, avoiding any tedious and complex "cell-broken" pretreatment procedures that might induce racemization of the d-X.
32885950	2	44	theme	cell	555:558	arg1	wall					560:563	the bacterial cell wall	541:563	the bacterial cell wall	541:563	We developed a biochemical lanthanide-encoding approach to quantify the uncanonical d-amino acid (d-X) that was edited in a small proportion into the terminal acyl-d-Ala-d-X of nascent peptidoglycan UDP-MurNAc-pentapeptides in the bacterial cell wall.
32885950	5	45	dep	158Gd	1082:1086	arg1	spectrometry					1174:1185	inductively coupled plasma mass spectrometry	1142:1185	inductively coupled plasma mass spectrometry	1142:1185	This approach allows direct quantification of the d-X in situ in the cell wall using 158Gd, 153Eu, and 154Sm species-unspecific isotope dilution inductively coupled plasma mass spectrometry, avoiding any tedious and complex "cell-broken" pretreatment procedures that might induce racemization of the d-X.
32885950	6	46	theme	antibiotic	1634:1643	arg1	mechanisms					1656:1665	the structure- and composition-dependent bacterial antibiotic resistance mechanisms	1583:1665	the structure- and composition-dependent bacterial antibiotic resistance mechanisms	1583:1665	The obtained site-specific and accurate in situ information about the d-X enables quantitative monitoring of the bacterial response when Staphylococcus aureus meets vancomycin, showing that the amounts of azide-d-Leu and ketone-d-Met assembled are more important after determining the structure- and composition-dependent bacterial antibiotic resistance mechanisms.
32885950	1	47	theme	invasion	222:229	arg1	defense					231:237	exogenous invasion defense	212:237	not only pathogenic attack but also exogenous invasion defense	176:237	A pathogenic bacterium has its own mechanisms for not only pathogenic attack but also exogenous invasion defense, in which the bacterial cell wall is the front line of attack and defense.
32885950	6	48	theme	structure-	1587:1596	arg1	mechanisms					1656:1665	the structure- and composition-dependent bacterial antibiotic resistance mechanisms	1583:1665	the structure- and composition-dependent bacterial antibiotic resistance mechanisms	1583:1665	The obtained site-specific and accurate in situ information about the d-X enables quantitative monitoring of the bacterial response when Staphylococcus aureus meets vancomycin, showing that the amounts of azide-d-Leu and ketone-d-Met assembled are more important after determining the structure- and composition-dependent bacterial antibiotic resistance mechanisms.
32885950	1	49	theme	attack	294:299	arg1	line					286:289	the front line	276:289	the front line of attack and defense	276:311	A pathogenic bacterium has its own mechanisms for not only pathogenic attack but also exogenous invasion defense, in which the bacterial cell wall is the front line of attack and defense.
32885950	1	49	theme	attack	294:299	arg1	wall					268:271	the bacterial cell wall	249:271	the bacterial cell wall	249:271	A pathogenic bacterium has its own mechanisms for not only pathogenic attack but also exogenous invasion defense, in which the bacterial cell wall is the front line of attack and defense.
32885950	2	50	theme	bacterial	545:553	arg1	wall					560:563	the bacterial cell wall	541:563	the bacterial cell wall	541:563	We developed a biochemical lanthanide-encoding approach to quantify the uncanonical d-amino acid (d-X) that was edited in a small proportion into the terminal acyl-d-Ala-d-X of nascent peptidoglycan UDP-MurNAc-pentapeptides in the bacterial cell wall.
32885950	5	51	theme	154Sm	1100:1104	arg1	dilution					1133:1140	154Sm species-unspecific isotope dilution	1100:1140	154Sm species-unspecific isotope dilution	1100:1140	This approach allows direct quantification of the d-X in situ in the cell wall using 158Gd, 153Eu, and 154Sm species-unspecific isotope dilution inductively coupled plasma mass spectrometry, avoiding any tedious and complex "cell-broken" pretreatment procedures that might induce racemization of the d-X.
32885950	5	52	theme	mass	1169:1172	arg1	spectrometry					1174:1185	inductively coupled plasma mass spectrometry	1142:1185	inductively coupled plasma mass spectrometry	1142:1185	This approach allows direct quantification of the d-X in situ in the cell wall using 158Gd, 153Eu, and 154Sm species-unspecific isotope dilution inductively coupled plasma mass spectrometry, avoiding any tedious and complex "cell-broken" pretreatment procedures that might induce racemization of the d-X.
32885950	3	53	theme	popular	671:677	arg1	imaging					687:693	the popular optical imaging	667:693	the popular optical imaging	667:693	This approach overcomes the difficulties regarding quantification and accuracy issues encountered by the popular optical imaging and traditional high-performance liquid chromatography-based methods.
32885950	5	54	theme	species-unspecific	1106:1123	arg1	dilution					1133:1140	154Sm species-unspecific isotope dilution	1100:1140	154Sm species-unspecific isotope dilution	1100:1140	This approach allows direct quantification of the d-X in situ in the cell wall using 158Gd, 153Eu, and 154Sm species-unspecific isotope dilution inductively coupled plasma mass spectrometry, avoiding any tedious and complex "cell-broken" pretreatment procedures that might induce racemization of the d-X.
32885950	1	55	theme	pathogenic	128:137	arg1	bacterium					139:147	A pathogenic bacterium	126:147	A pathogenic bacterium	126:147	A pathogenic bacterium has its own mechanisms for not only pathogenic attack but also exogenous invasion defense, in which the bacterial cell wall is the front line of attack and defense.
32885950	2	56	theme	small	438:442	arg1	proportion					444:453	a small proportion	436:453	a small proportion into the terminal acyl-d-Ala-d-X of nascent peptidoglycan UDP-MurNAc-pentapeptides in the bacterial cell wall	436:563	We developed a biochemical lanthanide-encoding approach to quantify the uncanonical d-amino acid (d-X) that was edited in a small proportion into the terminal acyl-d-Ala-d-X of nascent peptidoglycan UDP-MurNAc-pentapeptides in the bacterial cell wall.
32885950	1	57	theme	defense	305:311	arg1	line					286:289	the front line	276:289	the front line of attack and defense	276:311	A pathogenic bacterium has its own mechanisms for not only pathogenic attack but also exogenous invasion defense, in which the bacterial cell wall is the front line of attack and defense.
32885950	1	57	theme	defense	305:311	arg1	wall					268:271	the bacterial cell wall	249:271	the bacterial cell wall	249:271	A pathogenic bacterium has its own mechanisms for not only pathogenic attack but also exogenous invasion defense, in which the bacterial cell wall is the front line of attack and defense.
32885950	6	58	theme	response	1425:1432	arg1	monitoring					1397:1406	quantitative monitoring	1384:1406	quantitative monitoring of the bacterial response	1384:1432	The obtained site-specific and accurate in situ information about the d-X enables quantitative monitoring of the bacterial response when Staphylococcus aureus meets vancomycin, showing that the amounts of azide-d-Leu and ketone-d-Met assembled are more important after determining the structure- and composition-dependent bacterial antibiotic resistance mechanisms.
32885950	3	59	theme	high-performance	711:726	arg1	methods					756:762	traditional high-performance liquid chromatography-based methods	699:762	traditional high-performance liquid chromatography-based methods	699:762	This approach overcomes the difficulties regarding quantification and accuracy issues encountered by the popular optical imaging and traditional high-performance liquid chromatography-based methods.
32885950	5	60	theme	isotope	1125:1131	arg1	dilution					1133:1140	154Sm species-unspecific isotope dilution	1100:1140	154Sm species-unspecific isotope dilution	1100:1140	This approach allows direct quantification of the d-X in situ in the cell wall using 158Gd, 153Eu, and 154Sm species-unspecific isotope dilution inductively coupled plasma mass spectrometry, avoiding any tedious and complex "cell-broken" pretreatment procedures that might induce racemization of the d-X.
32885950	4	61	theme	synthesized	771:781	arg1	azide-d-Leu					783:793	Newly synthesized azide-d-Leu	765:793	Newly synthesized azide-d-Leu	765:793	Newly synthesized azide-d-Leu and ketone-d-Met were used together with alkynyl-d-Ala for their metabolic assembly and then bioorthogonally encoded by the correspondingly fabricated DBCO-DOTA-Gd, HNO-DOTA-Eu, and azide-DOTA-Sm tags.
32885950	7	62	theme	vancomycin	1761:1770	arg1	efficacy					1749:1756	the efficacy	1745:1756	the efficacy of vancomycin	1745:1770	In addition, we found that the combined use of vancomycin and d-Ala restores the efficacy of vancomycin and might be a wise and simple way to combat vancomycin intermediate-resistant S. aureus.
32885950	6	63	theme	bacterial	1415:1423	arg1	response					1425:1432	the bacterial response	1411:1432	the bacterial response	1411:1432	The obtained site-specific and accurate in situ information about the d-X enables quantitative monitoring of the bacterial response when Staphylococcus aureus meets vancomycin, showing that the amounts of azide-d-Leu and ketone-d-Met assembled are more important after determining the structure- and composition-dependent bacterial antibiotic resistance mechanisms.
32885950	3	64	theme	liquid	728:733	arg1	methods					756:762	traditional high-performance liquid chromatography-based methods	699:762	traditional high-performance liquid chromatography-based methods	699:762	This approach overcomes the difficulties regarding quantification and accuracy issues encountered by the popular optical imaging and traditional high-performance liquid chromatography-based methods.
32885950	4	65	dep	used	817:820	arg1	encoded					904:910	encoded	904:910	encoded by the correspondingly fabricated DBCO-DOTA-Gd, HNO-DOTA-Eu, and azide-DOTA-Sm tags	904:994	Newly synthesized azide-d-Leu and ketone-d-Met were used together with alkynyl-d-Ala for their metabolic assembly and then bioorthogonally encoded by the correspondingly fabricated DBCO-DOTA-Gd, HNO-DOTA-Eu, and azide-DOTA-Sm tags.
32885950	5	66	theme	direct	1018:1023	arg1	quantification					1025:1038	direct quantification	1018:1038	direct quantification of the d-X	1018:1049	This approach allows direct quantification of the d-X in situ in the cell wall using 158Gd, 153Eu, and 154Sm species-unspecific isotope dilution inductively coupled plasma mass spectrometry, avoiding any tedious and complex "cell-broken" pretreatment procedures that might induce racemization of the d-X.
32885950	6	67	theme	obtained	1306:1313	arg1	information					1350:1360	The obtained site-specific and accurate in situ information	1302:1360	The obtained site-specific and accurate in situ information about the d-X	1302:1374	The obtained site-specific and accurate in situ information about the d-X enables quantitative monitoring of the bacterial response when Staphylococcus aureus meets vancomycin, showing that the amounts of azide-d-Leu and ketone-d-Met assembled are more important after determining the structure- and composition-dependent bacterial antibiotic resistance mechanisms.
32885950	6	68	dep	obtained	1306:1313	arg1	accurate					1333:1340	accurate	1333:1340	accurate	1333:1340	The obtained site-specific and accurate in situ information about the d-X enables quantitative monitoring of the bacterial response when Staphylococcus aureus meets vancomycin, showing that the amounts of azide-d-Leu and ketone-d-Met assembled are more important after determining the structure- and composition-dependent bacterial antibiotic resistance mechanisms.
32885950	6	68	dep	obtained	1306:1313	arg1	site-specific					1315:1327	site-specific	1315:1327	site-specific	1315:1327	The obtained site-specific and accurate in situ information about the d-X enables quantitative monitoring of the bacterial response when Staphylococcus aureus meets vancomycin, showing that the amounts of azide-d-Leu and ketone-d-Met assembled are more important after determining the structure- and composition-dependent bacterial antibiotic resistance mechanisms.
32885950	6	68	dep	obtained	1306:1313	arg1	in					1342:1343	in	1342:1343	in	1342:1343	The obtained site-specific and accurate in situ information about the d-X enables quantitative monitoring of the bacterial response when Staphylococcus aureus meets vancomycin, showing that the amounts of azide-d-Leu and ketone-d-Met assembled are more important after determining the structure- and composition-dependent bacterial antibiotic resistance mechanisms.
32885950	6	69	theme	resistance	1645:1654	arg1	mechanisms					1656:1665	the structure- and composition-dependent bacterial antibiotic resistance mechanisms	1583:1665	the structure- and composition-dependent bacterial antibiotic resistance mechanisms	1583:1665	The obtained site-specific and accurate in situ information about the d-X enables quantitative monitoring of the bacterial response when Staphylococcus aureus meets vancomycin, showing that the amounts of azide-d-Leu and ketone-d-Met assembled are more important after determining the structure- and composition-dependent bacterial antibiotic resistance mechanisms.
32885950	0	70	theme	Bacterial	82:90	arg1	Response					92:99	the Bacterial Response	78:99	the Bacterial Response to Vancomycin Treatment	78:123	A Biochemical Lanthanide-Encoding Approach Enables Quantitative Monitoring of the Bacterial Response to Vancomycin Treatment.
32885950	3	71	theme	traditional	699:709	arg1	methods					756:762	traditional high-performance liquid chromatography-based methods	699:762	traditional high-performance liquid chromatography-based methods	699:762	This approach overcomes the difficulties regarding quantification and accuracy issues encountered by the popular optical imaging and traditional high-performance liquid chromatography-based methods.
32885950	3	72	theme	accuracy	636:643	arg1	issues					645:650	accuracy issues	636:650	accuracy issues	636:650	This approach overcomes the difficulties regarding quantification and accuracy issues encountered by the popular optical imaging and traditional high-performance liquid chromatography-based methods.
32885950	6	73	theme	bacterial	1624:1632	arg1	mechanisms					1656:1665	the structure- and composition-dependent bacterial antibiotic resistance mechanisms	1583:1665	the structure- and composition-dependent bacterial antibiotic resistance mechanisms	1583:1665	The obtained site-specific and accurate in situ information about the d-X enables quantitative monitoring of the bacterial response when Staphylococcus aureus meets vancomycin, showing that the amounts of azide-d-Leu and ketone-d-Met assembled are more important after determining the structure- and composition-dependent bacterial antibiotic resistance mechanisms.
32885950	1	74	theme	own	157:159	arg1	mechanisms					161:170	its own mechanisms	153:170	its own mechanisms	153:170	A pathogenic bacterium has its own mechanisms for not only pathogenic attack but also exogenous invasion defense, in which the bacterial cell wall is the front line of attack and defense.
32885950	2	75	theme	d-amino	398:404	arg1	d-X					412:414	d-X	412:414	d-X	412:414	We developed a biochemical lanthanide-encoding approach to quantify the uncanonical d-amino acid (d-X) that was edited in a small proportion into the terminal acyl-d-Ala-d-X of nascent peptidoglycan UDP-MurNAc-pentapeptides in the bacterial cell wall.
32885950	2	75	theme	d-amino	398:404	arg1	acid					406:409	the uncanonical d-amino acid	382:409	the uncanonical d-amino acid (d-X) that was edited in a small proportion into the terminal acyl-d-Ala-d-X of nascent peptidoglycan UDP-MurNAc-pentapeptides in the bacterial cell wall	382:563	We developed a biochemical lanthanide-encoding approach to quantify the uncanonical d-amino acid (d-X) that was edited in a small proportion into the terminal acyl-d-Ala-d-X of nascent peptidoglycan UDP-MurNAc-pentapeptides in the bacterial cell wall.
32885950	7	76	theme	d-Ala	1730:1734	arg1	use					1708:1710	the combined use	1695:1710	the combined use of vancomycin and d-Ala	1695:1734	In addition, we found that the combined use of vancomycin and d-Ala restores the efficacy of vancomycin and might be a wise and simple way to combat vancomycin intermediate-resistant S. aureus.
32885950	6	77	theme	azide-d-Leu	1507:1517	arg1	ketone-d-Met					1523:1534	ketone-d-Met	1523:1534	ketone-d-Met	1523:1534	The obtained site-specific and accurate in situ information about the d-X enables quantitative monitoring of the bacterial response when Staphylococcus aureus meets vancomycin, showing that the amounts of azide-d-Leu and ketone-d-Met assembled are more important after determining the structure- and composition-dependent bacterial antibiotic resistance mechanisms.
32885950	6	77	theme	azide-d-Leu	1507:1517	arg1	azide-d-Leu					1507:1517	azide-d-Leu	1507:1517	azide-d-Leu	1507:1517	The obtained site-specific and accurate in situ information about the d-X enables quantitative monitoring of the bacterial response when Staphylococcus aureus meets vancomycin, showing that the amounts of azide-d-Leu and ketone-d-Met assembled are more important after determining the structure- and composition-dependent bacterial antibiotic resistance mechanisms.
32885950	6	77	theme	azide-d-Leu	1507:1517	arg1	important					1555:1563	important	1555:1563	important	1555:1563	The obtained site-specific and accurate in situ information about the d-X enables quantitative monitoring of the bacterial response when Staphylococcus aureus meets vancomycin, showing that the amounts of azide-d-Leu and ketone-d-Met assembled are more important after determining the structure- and composition-dependent bacterial antibiotic resistance mechanisms.
32885950	6	77	theme	azide-d-Leu	1507:1517	arg1	amounts					1496:1502	the amounts	1492:1502	the amounts of azide-d-Leu and ketone-d-Met assembled	1492:1544	The obtained site-specific and accurate in situ information about the d-X enables quantitative monitoring of the bacterial response when Staphylococcus aureus meets vancomycin, showing that the amounts of azide-d-Leu and ketone-d-Met assembled are more important after determining the structure- and composition-dependent bacterial antibiotic resistance mechanisms.
32885950	6	78	theme	quantitative	1384:1395	arg1	monitoring					1397:1406	quantitative monitoring	1384:1406	quantitative monitoring of the bacterial response	1384:1432	The obtained site-specific and accurate in situ information about the d-X enables quantitative monitoring of the bacterial response when Staphylococcus aureus meets vancomycin, showing that the amounts of azide-d-Leu and ketone-d-Met assembled are more important after determining the structure- and composition-dependent bacterial antibiotic resistance mechanisms.
34606000	6	0	theme	immune	1039:1044	arg1	status					1046:1051	the immune status	1035:1051	the immune status after LPS exposure compared to controls	1035:1091	Looking at the immune status after LPS exposure compared to controls, a significantly higher percentage of B lymphocytes and decrease of CD4+CD25+ T cells were found in both subtypes, on dpp7 a significantly higher percentage of CD4 T+ cells in CD26+ pups, and a significantly higher percentage of monocytes in CD26- pups.
34606000	6	1	theme	B	1131:1131	arg1	lymphocytes					1133:1143	B lymphocytes	1131:1143	B lymphocytes	1131:1143	Looking at the immune status after LPS exposure compared to controls, a significantly higher percentage of B lymphocytes and decrease of CD4+CD25+ T cells were found in both subtypes, on dpp7 a significantly higher percentage of CD4 T+ cells in CD26+ pups, and a significantly higher percentage of monocytes in CD26- pups.
34606000	8	2	theme	CD26-	1587:1591	arg1	pups					1593:1596	CD26- pups	1587:1596	CD26- pups	1587:1596	Thus, daily postnatal exposition to low doses of LPS for 1 week resulted in a delay in formation of secondary septa, which remained up to dpp 14 in CD26- pups.
34606000	1	3	theme	multifactorial	147:160	arg1	Dipeptidyl-peptidase					113:132	Dipeptidyl-peptidase IV	113:135	Dipeptidyl-peptidase IV (CD26)	113:142	Dipeptidyl-peptidase IV (CD26), a multifactorial integral type II protein, is expressed in the lungs during development and is involved in inflammation processes.
34606000	1	3	theme	multifactorial	147:160	arg1	protein					179:185	a multifactorial integral type II protein	145:185	a multifactorial integral type II protein	145:185	Dipeptidyl-peptidase IV (CD26), a multifactorial integral type II protein, is expressed in the lungs during development and is involved in inflammation processes.
34606000	7	4	theme	T	1365:1365	arg1	cells					1367:1371	T cells	1365:1371	T cells	1365:1371	The percentage of T cells was significantly higher in the CD26-deficient group on each dpp.
34606000	5	5	theme	lung	814:817	arg1	volume					819:824	the lung volume	810:824	the lung volume	810:824	Daily LPS application did not change the lung volume but resulted in a significant retardation of alveolarization in both substrains proved by significantly lower values of septal surface and volume as well as higher mean free distances in airspaces.
34606000	1	6	theme	type	171:174	arg1	Dipeptidyl-peptidase					113:132	Dipeptidyl-peptidase IV	113:135	Dipeptidyl-peptidase IV (CD26)	113:142	Dipeptidyl-peptidase IV (CD26), a multifactorial integral type II protein, is expressed in the lungs during development and is involved in inflammation processes.
34606000	1	6	theme	type	171:174	arg1	protein					179:185	a multifactorial integral type II protein	145:185	a multifactorial integral type II protein	145:185	Dipeptidyl-peptidase IV (CD26), a multifactorial integral type II protein, is expressed in the lungs during development and is involved in inflammation processes.
34606000	2	7	from	retardation	349:359	arg1	development					383:393	morphological lung development	364:393	morphological lung development	364:393	We tested whether daily LPS administration influences the CD26-dependent retardation in morphological lung development and induces alterations in the immune status.
34606000	6	8	theme	cells	1260:1264	arg1	percentage					1239:1248	a significantly higher percentage	1216:1248	a significantly higher percentage of CD4 T+ cells in CD26+ pups	1216:1278	Looking at the immune status after LPS exposure compared to controls, a significantly higher percentage of B lymphocytes and decrease of CD4+CD25+ T cells were found in both subtypes, on dpp7 a significantly higher percentage of CD4 T+ cells in CD26+ pups, and a significantly higher percentage of monocytes in CD26- pups.
34606000	6	8	theme	cells	1260:1264	arg1	percentage					1308:1317	a significantly higher percentage	1285:1317	a significantly higher percentage of monocytes	1285:1330	Looking at the immune status after LPS exposure compared to controls, a significantly higher percentage of B lymphocytes and decrease of CD4+CD25+ T cells were found in both subtypes, on dpp7 a significantly higher percentage of CD4 T+ cells in CD26+ pups, and a significantly higher percentage of monocytes in CD26- pups.
34606000	3	9	theme	postpartum	555:564	arg1	dpp					567:569	postpartum (dpp) 3	555:572	days postpartum (dpp) 3 to 9	550:577	Newborn Fischer rats with and without CD26 deficiency were nebulized with 1 µg LPS/2 ml NaCl for 10 min from days postpartum (dpp) 3 to 9.
34606000	4	10	theme	activated	627:635	arg1	sorting					642:648	fluorescence activated cell sorting	614:648	fluorescence activated cell sorting (FACS)	614:655	We used stereological methods and fluorescence activated cell sorting (FACS) to determine morphological lung maturation and alterations in the pulmonary leukocyte content on dpp 7, 10, and 14.
34606000	4	10	theme	activated	627:635	arg1	FACS					651:654	FACS	651:654	FACS	651:654	We used stereological methods and fluorescence activated cell sorting (FACS) to determine morphological lung maturation and alterations in the pulmonary leukocyte content on dpp 7, 10, and 14.
34606000	6	11	theme	CD4	1253:1255	arg1	cells					1260:1264	CD4 T+ cells	1253:1264	CD4 T+ cells in CD26+ pups	1253:1278	Looking at the immune status after LPS exposure compared to controls, a significantly higher percentage of B lymphocytes and decrease of CD4+CD25+ T cells were found in both subtypes, on dpp7 a significantly higher percentage of CD4 T+ cells in CD26+ pups, and a significantly higher percentage of monocytes in CD26- pups.
34606000	5	12	theme	Daily	773:777	arg1	application					783:793	Daily LPS application	773:793	Daily LPS application	773:793	Daily LPS application did not change the lung volume but resulted in a significant retardation of alveolarization in both substrains proved by significantly lower values of septal surface and volume as well as higher mean free distances in airspaces.
34606000	6	13	theme	T	1171:1171	arg1	cells					1173:1177	CD4+CD25+ T cells	1161:1177	CD4+CD25+ T cells	1161:1177	Looking at the immune status after LPS exposure compared to controls, a significantly higher percentage of B lymphocytes and decrease of CD4+CD25+ T cells were found in both subtypes, on dpp7 a significantly higher percentage of CD4 T+ cells in CD26+ pups, and a significantly higher percentage of monocytes in CD26- pups.
34606000	8	14	from	delay	1517:1521	arg1	formation					1526:1534	formation	1526:1534	formation	1526:1534	Thus, daily postnatal exposition to low doses of LPS for 1 week resulted in a delay in formation of secondary septa, which remained up to dpp 14 in CD26- pups.
34606000	5	15	theme	mean	990:993	arg1	distances					1000:1008	higher mean free distances	983:1008	significantly lower values of septal surface and volume as well as higher mean free distances	916:1008	Daily LPS application did not change the lung volume but resulted in a significant retardation of alveolarization in both substrains proved by significantly lower values of septal surface and volume as well as higher mean free distances in airspaces.
34606000	6	16	theme	higher	1301:1306	arg1	percentage					1308:1317	a significantly higher percentage	1285:1317	a significantly higher percentage of monocytes	1285:1330	Looking at the immune status after LPS exposure compared to controls, a significantly higher percentage of B lymphocytes and decrease of CD4+CD25+ T cells were found in both subtypes, on dpp7 a significantly higher percentage of CD4 T+ cells in CD26+ pups, and a significantly higher percentage of monocytes in CD26- pups.
34606000	9	17	theme	pulmonary	1702:1710	arg1	composition					1724:1734	the pulmonary immune cell composition	1698:1734	the pulmonary immune cell composition	1698:1734	The retardation was accompanied by moderate parenchymal inflammation and CD26-dependent changes in the pulmonary immune cell composition.
34606000	4	18	theme	morphological	670:682	arg1	maturation					689:698	morphological lung maturation	670:698	morphological lung maturation	670:698	We used stereological methods and fluorescence activated cell sorting (FACS) to determine morphological lung maturation and alterations in the pulmonary leukocyte content on dpp 7, 10, and 14.
34606000	0	19	theme	rat	65:67	arg1	pups					69:72	rat pups	65:72	rat pups with and without CD26/DPP4 deficiency	65:110	Lung development and immune status under chronic LPS exposure in rat pups with and without CD26/DPP4 deficiency.
34606000	6	20	from	percentage	1308:1317	arg1	pups					1275:1278	CD26+ pups	1269:1278	CD26+ pups	1269:1278	Looking at the immune status after LPS exposure compared to controls, a significantly higher percentage of B lymphocytes and decrease of CD4+CD25+ T cells were found in both subtypes, on dpp7 a significantly higher percentage of CD4 T+ cells in CD26+ pups, and a significantly higher percentage of monocytes in CD26- pups.
34606000	3	21	dep	dpp	567:569	arg1	to					574:575	to	574:575	to	574:575	Newborn Fischer rats with and without CD26 deficiency were nebulized with 1 µg LPS/2 ml NaCl for 10 min from days postpartum (dpp) 3 to 9.
34606000	3	21	dep	dpp	567:569	arg1	9					577:577	9	577:577	9	577:577	Newborn Fischer rats with and without CD26 deficiency were nebulized with 1 µg LPS/2 ml NaCl for 10 min from days postpartum (dpp) 3 to 9.
34606000	9	22	theme	parenchymal	1643:1653	arg1	inflammation					1655:1666	moderate parenchymal inflammation	1634:1666	moderate parenchymal inflammation	1634:1666	The retardation was accompanied by moderate parenchymal inflammation and CD26-dependent changes in the pulmonary immune cell composition.
34606000	6	23	from	pups	1275:1278	arg1	percentage					1239:1248	a significantly higher percentage	1216:1248	a significantly higher percentage of CD4 T+ cells in CD26+ pups	1216:1278	Looking at the immune status after LPS exposure compared to controls, a significantly higher percentage of B lymphocytes and decrease of CD4+CD25+ T cells were found in both subtypes, on dpp7 a significantly higher percentage of CD4 T+ cells in CD26+ pups, and a significantly higher percentage of monocytes in CD26- pups.
34606000	6	23	from	pups	1275:1278	arg1	percentage					1308:1317	a significantly higher percentage	1285:1317	a significantly higher percentage of monocytes	1285:1330	Looking at the immune status after LPS exposure compared to controls, a significantly higher percentage of B lymphocytes and decrease of CD4+CD25+ T cells were found in both subtypes, on dpp7 a significantly higher percentage of CD4 T+ cells in CD26+ pups, and a significantly higher percentage of monocytes in CD26- pups.
34606000	5	24	theme	lower	930:934	arg1	values					936:941	significantly lower values	916:941	significantly lower values of septal surface and volume as well as higher mean free distances	916:1008	Daily LPS application did not change the lung volume but resulted in a significant retardation of alveolarization in both substrains proved by significantly lower values of septal surface and volume as well as higher mean free distances in airspaces.
34606000	6	25	theme	CD26+	1269:1273	arg1	pups					1275:1278	CD26+ pups	1269:1278	CD26+ pups	1269:1278	Looking at the immune status after LPS exposure compared to controls, a significantly higher percentage of B lymphocytes and decrease of CD4+CD25+ T cells were found in both subtypes, on dpp7 a significantly higher percentage of CD4 T+ cells in CD26+ pups, and a significantly higher percentage of monocytes in CD26- pups.
34606000	6	26	located	found	1184:1188	arg2	percentage					1117:1126	a significantly higher percentage	1094:1126	a significantly higher percentage of B lymphocytes	1094:1143	Looking at the immune status after LPS exposure compared to controls, a significantly higher percentage of B lymphocytes and decrease of CD4+CD25+ T cells were found in both subtypes, on dpp7 a significantly higher percentage of CD4 T+ cells in CD26+ pups, and a significantly higher percentage of monocytes in CD26- pups.
34606000	6	26	located	found	1184:1188	arg1	subtypes					1198:1205	both subtypes	1193:1205	both subtypes	1193:1205	Looking at the immune status after LPS exposure compared to controls, a significantly higher percentage of B lymphocytes and decrease of CD4+CD25+ T cells were found in both subtypes, on dpp7 a significantly higher percentage of CD4 T+ cells in CD26+ pups, and a significantly higher percentage of monocytes in CD26- pups.
34606000	6	26	located	found	1184:1188	arg2	decrease					1149:1156	decrease	1149:1156	decrease of CD4+CD25+ T cells	1149:1177	Looking at the immune status after LPS exposure compared to controls, a significantly higher percentage of B lymphocytes and decrease of CD4+CD25+ T cells were found in both subtypes, on dpp7 a significantly higher percentage of CD4 T+ cells in CD26+ pups, and a significantly higher percentage of monocytes in CD26- pups.
34606000	0	27	from	status	28:33	arg1	pups					69:72	rat pups	65:72	rat pups with and without CD26/DPP4 deficiency	65:110	Lung development and immune status under chronic LPS exposure in rat pups with and without CD26/DPP4 deficiency.
34606000	2	28	theme	lung	378:381	arg1	development					383:393	morphological lung development	364:393	morphological lung development	364:393	We tested whether daily LPS administration influences the CD26-dependent retardation in morphological lung development and induces alterations in the immune status.
34606000	0	29	theme	Lung	0:3	arg1	development					5:15	Lung development	0:15	Lung development	0:15	Lung development and immune status under chronic LPS exposure in rat pups with and without CD26/DPP4 deficiency.
34606000	5	30	from	retardation	856:866	arg1	substrains					895:904	both substrains	890:904	both substrains proved by significantly lower values of septal surface and volume as well as higher mean free distances in airspaces	890:1021	Daily LPS application did not change the lung volume but resulted in a significant retardation of alveolarization in both substrains proved by significantly lower values of septal surface and volume as well as higher mean free distances in airspaces.
34606000	8	31	theme	daily	1445:1449	arg1	exposition					1461:1470	daily postnatal exposition	1445:1470	daily postnatal exposition to low doses of LPS for 1 week	1445:1501	Thus, daily postnatal exposition to low doses of LPS for 1 week resulted in a delay in formation of secondary septa, which remained up to dpp 14 in CD26- pups.
34606000	8	32	dep	dpp	1577:1579	arg1	up					1571:1572	up	1571:1572	up	1571:1572	Thus, daily postnatal exposition to low doses of LPS for 1 week resulted in a delay in formation of secondary septa, which remained up to dpp 14 in CD26- pups.
34606000	4	33	theme	leukocyte	733:741	arg1	content					743:749	the pulmonary leukocyte content	719:749	the pulmonary leukocyte content on dpp 7, 10, and 14	719:770	We used stereological methods and fluorescence activated cell sorting (FACS) to determine morphological lung maturation and alterations in the pulmonary leukocyte content on dpp 7, 10, and 14.
34606000	5	34	theme	LPS	779:781	arg1	application					783:793	Daily LPS application	773:793	Daily LPS application	773:793	Daily LPS application did not change the lung volume but resulted in a significant retardation of alveolarization in both substrains proved by significantly lower values of septal surface and volume as well as higher mean free distances in airspaces.
34606000	1	35	theme	inflammation	252:263	arg1	processes					265:273	inflammation processes	252:273	inflammation processes	252:273	Dipeptidyl-peptidase IV (CD26), a multifactorial integral type II protein, is expressed in the lungs during development and is involved in inflammation processes.
34606000	9	36	theme	immune	1712:1717	arg1	composition					1724:1734	the pulmonary immune cell composition	1698:1734	the pulmonary immune cell composition	1698:1734	The retardation was accompanied by moderate parenchymal inflammation and CD26-dependent changes in the pulmonary immune cell composition.
34606000	3	37	theme	Fischer	449:455	arg1	rats					457:460	Newborn Fischer rats	441:460	Newborn Fischer rats with and without CD26 deficiency	441:493	Newborn Fischer rats with and without CD26 deficiency were nebulized with 1 µg LPS/2 ml NaCl for 10 min from days postpartum (dpp) 3 to 9.
34606000	8	38	theme	low	1475:1477	arg1	doses					1479:1483	low doses	1475:1483	low doses of LPS	1475:1490	Thus, daily postnatal exposition to low doses of LPS for 1 week resulted in a delay in formation of secondary septa, which remained up to dpp 14 in CD26- pups.
34606000	0	39	theme	chronic	41:47	arg1	exposure					53:60	chronic LPS exposure	41:60	chronic LPS exposure	41:60	Lung development and immune status under chronic LPS exposure in rat pups with and without CD26/DPP4 deficiency.
34606000	5	40	theme	higher	983:988	arg1	distances					1000:1008	higher mean free distances	983:1008	significantly lower values of septal surface and volume as well as higher mean free distances	916:1008	Daily LPS application did not change the lung volume but resulted in a significant retardation of alveolarization in both substrains proved by significantly lower values of septal surface and volume as well as higher mean free distances in airspaces.
34606000	2	41	theme	LPS	300:302	arg1	administration					304:317	daily LPS administration	294:317	daily LPS administration	294:317	We tested whether daily LPS administration influences the CD26-dependent retardation in morphological lung development and induces alterations in the immune status.
34606000	7	42	theme	CD26-deficient	1405:1418	arg1	group					1420:1424	the CD26-deficient group	1401:1424	the CD26-deficient group on each dpp	1401:1436	The percentage of T cells was significantly higher in the CD26-deficient group on each dpp.
34606000	5	43	theme	surface	953:959	arg1	distances					1000:1008	higher mean free distances	983:1008	significantly lower values of septal surface and volume as well as higher mean free distances	916:1008	Daily LPS application did not change the lung volume but resulted in a significant retardation of alveolarization in both substrains proved by significantly lower values of septal surface and volume as well as higher mean free distances in airspaces.
34606000	5	43	theme	surface	953:959	arg1	values					936:941	significantly lower values	916:941	significantly lower values of septal surface and volume as well as higher mean free distances	916:1008	Daily LPS application did not change the lung volume but resulted in a significant retardation of alveolarization in both substrains proved by significantly lower values of septal surface and volume as well as higher mean free distances in airspaces.
34606000	2	44	from	alterations	407:417	arg1	status					433:438	the immune status	422:438	the immune status	422:438	We tested whether daily LPS administration influences the CD26-dependent retardation in morphological lung development and induces alterations in the immune status.
34606000	6	45	theme	LPS	1059:1061	arg1	exposure					1063:1070	LPS exposure	1059:1070	LPS exposure compared to controls	1059:1091	Looking at the immune status after LPS exposure compared to controls, a significantly higher percentage of B lymphocytes and decrease of CD4+CD25+ T cells were found in both subtypes, on dpp7 a significantly higher percentage of CD4 T+ cells in CD26+ pups, and a significantly higher percentage of monocytes in CD26- pups.
34606000	3	46	theme	1 µg	515:518	arg1	LPS/2 ml					520:527	LPS/2 ml	520:527	LPS/2 ml	520:527	Newborn Fischer rats with and without CD26 deficiency were nebulized with 1 µg LPS/2 ml NaCl for 10 min from days postpartum (dpp) 3 to 9.
34606000	8	47	theme	septa	1549:1553	arg1	formation					1526:1534	formation	1526:1534	formation	1526:1534	Thus, daily postnatal exposition to low doses of LPS for 1 week resulted in a delay in formation of secondary septa, which remained up to dpp 14 in CD26- pups.
34606000	7	48	from	group	1420:1424	arg1	dpp					1434:1436	each dpp	1429:1436	each dpp	1429:1436	The percentage of T cells was significantly higher in the CD26-deficient group on each dpp.
34606000	5	49	theme	volume	965:970	arg1	distances					1000:1008	higher mean free distances	983:1008	significantly lower values of septal surface and volume as well as higher mean free distances	916:1008	Daily LPS application did not change the lung volume but resulted in a significant retardation of alveolarization in both substrains proved by significantly lower values of septal surface and volume as well as higher mean free distances in airspaces.
34606000	5	49	theme	volume	965:970	arg1	values					936:941	significantly lower values	916:941	significantly lower values of septal surface and volume as well as higher mean free distances	916:1008	Daily LPS application did not change the lung volume but resulted in a significant retardation of alveolarization in both substrains proved by significantly lower values of septal surface and volume as well as higher mean free distances in airspaces.
34606000	2	50	theme	CD26-dependent	334:347	arg1	retardation					349:359	the CD26-dependent retardation	330:359	the CD26-dependent retardation in morphological lung development	330:393	We tested whether daily LPS administration influences the CD26-dependent retardation in morphological lung development and induces alterations in the immune status.
34606000	7	51	theme	cells	1367:1371	arg1	higher					1391:1396	higher	1391:1396	higher	1391:1396	The percentage of T cells was significantly higher in the CD26-deficient group on each dpp.
34606000	7	51	theme	cells	1367:1371	arg1	percentage					1351:1360	The percentage	1347:1360	The percentage of T cells	1347:1371	The percentage of T cells was significantly higher in the CD26-deficient group on each dpp.
34606000	9	52	from	changes	1687:1693	arg1	composition					1724:1734	the pulmonary immune cell composition	1698:1734	the pulmonary immune cell composition	1698:1734	The retardation was accompanied by moderate parenchymal inflammation and CD26-dependent changes in the pulmonary immune cell composition.
34606000	6	53	theme	higher	1110:1115	arg1	percentage					1117:1126	a significantly higher percentage	1094:1126	a significantly higher percentage of B lymphocytes	1094:1143	Looking at the immune status after LPS exposure compared to controls, a significantly higher percentage of B lymphocytes and decrease of CD4+CD25+ T cells were found in both subtypes, on dpp7 a significantly higher percentage of CD4 T+ cells in CD26+ pups, and a significantly higher percentage of monocytes in CD26- pups.
34606000	4	54	theme	cell	637:640	arg1	sorting					642:648	fluorescence activated cell sorting	614:648	fluorescence activated cell sorting (FACS)	614:655	We used stereological methods and fluorescence activated cell sorting (FACS) to determine morphological lung maturation and alterations in the pulmonary leukocyte content on dpp 7, 10, and 14.
34606000	4	54	theme	cell	637:640	arg1	FACS					651:654	FACS	651:654	FACS	651:654	We used stereological methods and fluorescence activated cell sorting (FACS) to determine morphological lung maturation and alterations in the pulmonary leukocyte content on dpp 7, 10, and 14.
34606000	1	55	theme	integral	162:169	arg1	Dipeptidyl-peptidase					113:132	Dipeptidyl-peptidase IV	113:135	Dipeptidyl-peptidase IV (CD26)	113:142	Dipeptidyl-peptidase IV (CD26), a multifactorial integral type II protein, is expressed in the lungs during development and is involved in inflammation processes.
34606000	1	55	theme	integral	162:169	arg1	protein					179:185	a multifactorial integral type II protein	145:185	a multifactorial integral type II protein	145:185	Dipeptidyl-peptidase IV (CD26), a multifactorial integral type II protein, is expressed in the lungs during development and is involved in inflammation processes.
34606000	4	56	used	used	583:586	arg2	We					580:581	We	580:581	We	580:581	We used stereological methods and fluorescence activated cell sorting (FACS) to determine morphological lung maturation and alterations in the pulmonary leukocyte content on dpp 7, 10, and 14.
34606000	1	57	theme	II	176:177	arg1	Dipeptidyl-peptidase					113:132	Dipeptidyl-peptidase IV	113:135	Dipeptidyl-peptidase IV (CD26)	113:142	Dipeptidyl-peptidase IV (CD26), a multifactorial integral type II protein, is expressed in the lungs during development and is involved in inflammation processes.
34606000	1	57	theme	II	176:177	arg1	protein					179:185	a multifactorial integral type II protein	145:185	a multifactorial integral type II protein	145:185	Dipeptidyl-peptidase IV (CD26), a multifactorial integral type II protein, is expressed in the lungs during development and is involved in inflammation processes.
34606000	5	58	theme	significant	844:854	arg1	retardation					856:866	a significant retardation	842:866	a significant retardation of alveolarization in both substrains proved by significantly lower values of septal surface and volume as well as higher mean free distances in airspaces	842:1021	Daily LPS application did not change the lung volume but resulted in a significant retardation of alveolarization in both substrains proved by significantly lower values of septal surface and volume as well as higher mean free distances in airspaces.
34606000	6	59	theme	T+	1257:1258	arg1	cells					1260:1264	CD4 T+ cells	1253:1264	CD4 T+ cells in CD26+ pups	1253:1278	Looking at the immune status after LPS exposure compared to controls, a significantly higher percentage of B lymphocytes and decrease of CD4+CD25+ T cells were found in both subtypes, on dpp7 a significantly higher percentage of CD4 T+ cells in CD26+ pups, and a significantly higher percentage of monocytes in CD26- pups.
34606000	8	60	theme	secondary	1539:1547	arg1	septa					1549:1553	secondary septa	1539:1553	secondary septa	1539:1553	Thus, daily postnatal exposition to low doses of LPS for 1 week resulted in a delay in formation of secondary septa, which remained up to dpp 14 in CD26- pups.
34606000	6	61	theme	cells	1173:1177	arg1	decrease					1149:1156	decrease	1149:1156	decrease of CD4+CD25+ T cells	1149:1177	Looking at the immune status after LPS exposure compared to controls, a significantly higher percentage of B lymphocytes and decrease of CD4+CD25+ T cells were found in both subtypes, on dpp7 a significantly higher percentage of CD4 T+ cells in CD26+ pups, and a significantly higher percentage of monocytes in CD26- pups.
34606000	6	61	theme	cells	1173:1177	arg1	percentage					1117:1126	a significantly higher percentage	1094:1126	a significantly higher percentage of B lymphocytes	1094:1143	Looking at the immune status after LPS exposure compared to controls, a significantly higher percentage of B lymphocytes and decrease of CD4+CD25+ T cells were found in both subtypes, on dpp7 a significantly higher percentage of CD4 T+ cells in CD26+ pups, and a significantly higher percentage of monocytes in CD26- pups.
34606000	4	62	theme	fluorescence	614:625	arg1	sorting					642:648	fluorescence activated cell sorting	614:648	fluorescence activated cell sorting (FACS)	614:655	We used stereological methods and fluorescence activated cell sorting (FACS) to determine morphological lung maturation and alterations in the pulmonary leukocyte content on dpp 7, 10, and 14.
34606000	4	62	theme	fluorescence	614:625	arg1	FACS					651:654	FACS	651:654	FACS	651:654	We used stereological methods and fluorescence activated cell sorting (FACS) to determine morphological lung maturation and alterations in the pulmonary leukocyte content on dpp 7, 10, and 14.
34606000	6	63	theme	monocytes	1322:1330	arg1	percentage					1239:1248	a significantly higher percentage	1216:1248	a significantly higher percentage of CD4 T+ cells in CD26+ pups	1216:1278	Looking at the immune status after LPS exposure compared to controls, a significantly higher percentage of B lymphocytes and decrease of CD4+CD25+ T cells were found in both subtypes, on dpp7 a significantly higher percentage of CD4 T+ cells in CD26+ pups, and a significantly higher percentage of monocytes in CD26- pups.
34606000	6	63	theme	monocytes	1322:1330	arg1	percentage					1308:1317	a significantly higher percentage	1285:1317	a significantly higher percentage of monocytes	1285:1330	Looking at the immune status after LPS exposure compared to controls, a significantly higher percentage of B lymphocytes and decrease of CD4+CD25+ T cells were found in both subtypes, on dpp7 a significantly higher percentage of CD4 T+ cells in CD26+ pups, and a significantly higher percentage of monocytes in CD26- pups.
34606000	6	64	theme	CD4+CD25+	1161:1169	arg1	cells					1173:1177	CD4+CD25+ T cells	1161:1177	CD4+CD25+ T cells	1161:1177	Looking at the immune status after LPS exposure compared to controls, a significantly higher percentage of B lymphocytes and decrease of CD4+CD25+ T cells were found in both subtypes, on dpp7 a significantly higher percentage of CD4 T+ cells in CD26+ pups, and a significantly higher percentage of monocytes in CD26- pups.
34606000	4	65	theme	lung	684:687	arg1	maturation					689:698	morphological lung maturation	670:698	morphological lung maturation	670:698	We used stereological methods and fluorescence activated cell sorting (FACS) to determine morphological lung maturation and alterations in the pulmonary leukocyte content on dpp 7, 10, and 14.
34606000	3	66	from	days	550:553	arg1	10 min					538:543	10 min	538:543	10 min from days postpartum (dpp) 3 to 9	538:577	Newborn Fischer rats with and without CD26 deficiency were nebulized with 1 µg LPS/2 ml NaCl for 10 min from days postpartum (dpp) 3 to 9.
34606000	6	67	theme	higher	1232:1237	arg1	percentage					1239:1248	a significantly higher percentage	1216:1248	a significantly higher percentage of CD4 T+ cells in CD26+ pups	1216:1278	Looking at the immune status after LPS exposure compared to controls, a significantly higher percentage of B lymphocytes and decrease of CD4+CD25+ T cells were found in both subtypes, on dpp7 a significantly higher percentage of CD4 T+ cells in CD26+ pups, and a significantly higher percentage of monocytes in CD26- pups.
34606000	5	68	theme	free	995:998	arg1	distances					1000:1008	higher mean free distances	983:1008	significantly lower values of septal surface and volume as well as higher mean free distances	916:1008	Daily LPS application did not change the lung volume but resulted in a significant retardation of alveolarization in both substrains proved by significantly lower values of septal surface and volume as well as higher mean free distances in airspaces.
34606000	5	69	theme	alveolarization	871:885	arg1	retardation					856:866	a significant retardation	842:866	a significant retardation of alveolarization in both substrains proved by significantly lower values of septal surface and volume as well as higher mean free distances in airspaces	842:1021	Daily LPS application did not change the lung volume but resulted in a significant retardation of alveolarization in both substrains proved by significantly lower values of septal surface and volume as well as higher mean free distances in airspaces.
34606000	3	70	dep	days	550:553	arg1	dpp					567:569	postpartum (dpp) 3	555:572	days postpartum (dpp) 3 to 9	550:577	Newborn Fischer rats with and without CD26 deficiency were nebulized with 1 µg LPS/2 ml NaCl for 10 min from days postpartum (dpp) 3 to 9.
34606000	6	71	theme	lymphocytes	1133:1143	arg1	decrease					1149:1156	decrease	1149:1156	decrease of CD4+CD25+ T cells	1149:1177	Looking at the immune status after LPS exposure compared to controls, a significantly higher percentage of B lymphocytes and decrease of CD4+CD25+ T cells were found in both subtypes, on dpp7 a significantly higher percentage of CD4 T+ cells in CD26+ pups, and a significantly higher percentage of monocytes in CD26- pups.
34606000	6	71	theme	lymphocytes	1133:1143	arg1	percentage					1117:1126	a significantly higher percentage	1094:1126	a significantly higher percentage of B lymphocytes	1094:1143	Looking at the immune status after LPS exposure compared to controls, a significantly higher percentage of B lymphocytes and decrease of CD4+CD25+ T cells were found in both subtypes, on dpp7 a significantly higher percentage of CD4 T+ cells in CD26+ pups, and a significantly higher percentage of monocytes in CD26- pups.
34606000	9	72	theme	moderate	1634:1641	arg1	inflammation					1655:1666	moderate parenchymal inflammation	1634:1666	moderate parenchymal inflammation	1634:1666	The retardation was accompanied by moderate parenchymal inflammation and CD26-dependent changes in the pulmonary immune cell composition.
34606000	0	73	from	development	5:15	arg1	pups					69:72	rat pups	65:72	rat pups with and without CD26/DPP4 deficiency	65:110	Lung development and immune status under chronic LPS exposure in rat pups with and without CD26/DPP4 deficiency.
34606000	4	74	from	dpp	754:756	arg1	maturation					689:698	morphological lung maturation	670:698	morphological lung maturation	670:698	We used stereological methods and fluorescence activated cell sorting (FACS) to determine morphological lung maturation and alterations in the pulmonary leukocyte content on dpp 7, 10, and 14.
34606000	4	74	from	dpp	754:756	arg1	alterations					704:714	alterations	704:714	alterations in the pulmonary leukocyte content on dpp 7, 10, and 14	704:770	We used stereological methods and fluorescence activated cell sorting (FACS) to determine morphological lung maturation and alterations in the pulmonary leukocyte content on dpp 7, 10, and 14.
34606000	2	75	theme	daily	294:298	arg1	administration					304:317	daily LPS administration	294:317	daily LPS administration	294:317	We tested whether daily LPS administration influences the CD26-dependent retardation in morphological lung development and induces alterations in the immune status.
34606000	0	76	theme	CD26/DPP4	91:99	arg1	deficiency					101:110	CD26/DPP4 deficiency	91:110	CD26/DPP4 deficiency	91:110	Lung development and immune status under chronic LPS exposure in rat pups with and without CD26/DPP4 deficiency.
34606000	4	77	from	content	743:749	arg1	dpp					754:756	dpp 7, 10, and 14	754:770	dpp 7, 10, and 14	754:770	We used stereological methods and fluorescence activated cell sorting (FACS) to determine morphological lung maturation and alterations in the pulmonary leukocyte content on dpp 7, 10, and 14.
34606000	9	78	theme	CD26-dependent	1672:1685	arg1	changes					1687:1693	CD26-dependent changes	1672:1693	CD26-dependent changes	1672:1693	The retardation was accompanied by moderate parenchymal inflammation and CD26-dependent changes in the pulmonary immune cell composition.
34606000	3	79	theme	Newborn	441:447	arg1	rats					457:460	Newborn Fischer rats	441:460	Newborn Fischer rats with and without CD26 deficiency	441:493	Newborn Fischer rats with and without CD26 deficiency were nebulized with 1 µg LPS/2 ml NaCl for 10 min from days postpartum (dpp) 3 to 9.
34606000	8	80	theme	postnatal	1451:1459	arg1	exposition					1461:1470	daily postnatal exposition	1445:1470	daily postnatal exposition to low doses of LPS for 1 week	1445:1501	Thus, daily postnatal exposition to low doses of LPS for 1 week resulted in a delay in formation of secondary septa, which remained up to dpp 14 in CD26- pups.
34606000	2	81	theme	morphological	364:376	arg1	development					383:393	morphological lung development	364:393	morphological lung development	364:393	We tested whether daily LPS administration influences the CD26-dependent retardation in morphological lung development and induces alterations in the immune status.
34606000	0	82	theme	immune	21:26	arg1	status					28:33	immune status	21:33	immune status	21:33	Lung development and immune status under chronic LPS exposure in rat pups with and without CD26/DPP4 deficiency.
34606000	2	83	theme	immune	426:431	arg1	status					433:438	the immune status	422:438	the immune status	422:438	We tested whether daily LPS administration influences the CD26-dependent retardation in morphological lung development and induces alterations in the immune status.
34606000	4	84	theme	pulmonary	723:731	arg1	content					743:749	the pulmonary leukocyte content	719:749	the pulmonary leukocyte content on dpp 7, 10, and 14	719:770	We used stereological methods and fluorescence activated cell sorting (FACS) to determine morphological lung maturation and alterations in the pulmonary leukocyte content on dpp 7, 10, and 14.
34606000	6	85	theme	CD26-	1335:1339	arg1	pups					1341:1344	CD26- pups	1335:1344	CD26- pups	1335:1344	Looking at the immune status after LPS exposure compared to controls, a significantly higher percentage of B lymphocytes and decrease of CD4+CD25+ T cells were found in both subtypes, on dpp7 a significantly higher percentage of CD4 T+ cells in CD26+ pups, and a significantly higher percentage of monocytes in CD26- pups.
34606000	6	86	from	cells	1260:1264	arg1	pups					1275:1278	CD26+ pups	1269:1278	CD26+ pups	1269:1278	Looking at the immune status after LPS exposure compared to controls, a significantly higher percentage of B lymphocytes and decrease of CD4+CD25+ T cells were found in both subtypes, on dpp7 a significantly higher percentage of CD4 T+ cells in CD26+ pups, and a significantly higher percentage of monocytes in CD26- pups.
34606000	9	87	theme	cell	1719:1722	arg1	composition					1724:1734	the pulmonary immune cell composition	1698:1734	the pulmonary immune cell composition	1698:1734	The retardation was accompanied by moderate parenchymal inflammation and CD26-dependent changes in the pulmonary immune cell composition.
34606000	0	88	theme	LPS	49:51	arg1	exposure					53:60	chronic LPS exposure	41:60	chronic LPS exposure	41:60	Lung development and immune status under chronic LPS exposure in rat pups with and without CD26/DPP4 deficiency.
34606000	9	89	from	inflammation	1655:1666	arg1	composition					1724:1734	the pulmonary immune cell composition	1698:1734	the pulmonary immune cell composition	1698:1734	The retardation was accompanied by moderate parenchymal inflammation and CD26-dependent changes in the pulmonary immune cell composition.
34606000	8	90	theme	LPS	1488:1490	arg1	doses					1479:1483	low doses	1475:1483	low doses of LPS	1475:1490	Thus, daily postnatal exposition to low doses of LPS for 1 week resulted in a delay in formation of secondary septa, which remained up to dpp 14 in CD26- pups.
34606000	4	91	from	alterations	704:714	arg1	dpp					754:756	dpp 7, 10, and 14	754:770	dpp 7, 10, and 14	754:770	We used stereological methods and fluorescence activated cell sorting (FACS) to determine morphological lung maturation and alterations in the pulmonary leukocyte content on dpp 7, 10, and 14.
34606000	4	91	from	alterations	704:714	arg1	content					743:749	the pulmonary leukocyte content	719:749	the pulmonary leukocyte content on dpp 7, 10, and 14	719:770	We used stereological methods and fluorescence activated cell sorting (FACS) to determine morphological lung maturation and alterations in the pulmonary leukocyte content on dpp 7, 10, and 14.
34606000	6	92	from	percentage	1239:1248	arg1	pups					1275:1278	CD26+ pups	1269:1278	CD26+ pups	1269:1278	Looking at the immune status after LPS exposure compared to controls, a significantly higher percentage of B lymphocytes and decrease of CD4+CD25+ T cells were found in both subtypes, on dpp7 a significantly higher percentage of CD4 T+ cells in CD26+ pups, and a significantly higher percentage of monocytes in CD26- pups.
34606000	5	93	theme	septal	946:951	arg1	surface					953:959	septal surface	946:959	septal surface	946:959	Daily LPS application did not change the lung volume but resulted in a significant retardation of alveolarization in both substrains proved by significantly lower values of septal surface and volume as well as higher mean free distances in airspaces.
34606000	3	94	theme	CD26	479:482	arg1	deficiency					484:493	CD26 deficiency	479:493	CD26 deficiency	479:493	Newborn Fischer rats with and without CD26 deficiency were nebulized with 1 µg LPS/2 ml NaCl for 10 min from days postpartum (dpp) 3 to 9.
34606000	4	95	from	maturation	689:698	arg1	dpp					754:756	dpp 7, 10, and 14	754:770	dpp 7, 10, and 14	754:770	We used stereological methods and fluorescence activated cell sorting (FACS) to determine morphological lung maturation and alterations in the pulmonary leukocyte content on dpp 7, 10, and 14.
34606000	4	95	from	maturation	689:698	arg1	content					743:749	the pulmonary leukocyte content	719:749	the pulmonary leukocyte content on dpp 7, 10, and 14	719:770	We used stereological methods and fluorescence activated cell sorting (FACS) to determine morphological lung maturation and alterations in the pulmonary leukocyte content on dpp 7, 10, and 14.
34606000	4	96	theme	stereological	588:600	arg1	methods					602:608	stereological methods	588:608	stereological methods	588:608	We used stereological methods and fluorescence activated cell sorting (FACS) to determine morphological lung maturation and alterations in the pulmonary leukocyte content on dpp 7, 10, and 14.
34606000	3	97	theme	LPS/2 ml	520:527	arg1	NaCl					529:532	1 µg LPS/2 ml NaCl	515:532	1 µg LPS/2 ml NaCl	515:532	Newborn Fischer rats with and without CD26 deficiency were nebulized with 1 µg LPS/2 ml NaCl for 10 min from days postpartum (dpp) 3 to 9.
34606000	6	98	from	dpp7	1211:1214	arg1	pups					1341:1344	CD26- pups	1335:1344	CD26- pups	1335:1344	Looking at the immune status after LPS exposure compared to controls, a significantly higher percentage of B lymphocytes and decrease of CD4+CD25+ T cells were found in both subtypes, on dpp7 a significantly higher percentage of CD4 T+ cells in CD26+ pups, and a significantly higher percentage of monocytes in CD26- pups.
34241591	8	0	theme	cellular	1025:1032	arg1	acids					1040:1044	the predominant cellular fatty acids	1009:1044	the predominant cellular fatty acids	1009:1044	Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-8(H4) and the predominant cellular fatty acids were iso-C16 : 0, 10-methyl-C17 : 0 and C18 : 1 ω9c.
34241591	8	0	theme	cellular	1025:1032	arg1	iso-C16 					1051:1058	iso-C16 	1051:1058	iso-C16 	1051:1058	Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-8(H4) and the predominant cellular fatty acids were iso-C16 : 0, 10-methyl-C17 : 0 and C18 : 1 ω9c.
34241591	8	1	dep	iso-C16 	1051:1058	arg1	ω9c					1094:1096	 1 ω9c	1091:1096	iso-C16 : 0, 10-methyl-C17 : 0 and C18 : 1 ω9c	1051:1096	Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-8(H4) and the predominant cellular fatty acids were iso-C16 : 0, 10-methyl-C17 : 0 and C18 : 1 ω9c.
34241591	8	1	dep	iso-C16 	1051:1058	arg1	 0					1079:1080	 0	1079:1080	 0	1079:1080	Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-8(H4) and the predominant cellular fatty acids were iso-C16 : 0, 10-methyl-C17 : 0 and C18 : 1 ω9c.
34241591	8	1	dep	iso-C16 	1051:1058	arg1	 0					1060:1061	 0	1060:1061	 0	1060:1061	Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-8(H4) and the predominant cellular fatty acids were iso-C16 : 0, 10-methyl-C17 : 0 and C18 : 1 ω9c.
34241591	8	1	dep	iso-C16 	1051:1058	arg1	C18 					1086:1089	C18 	1086:1089	C18 	1086:1089	Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-8(H4) and the predominant cellular fatty acids were iso-C16 : 0, 10-methyl-C17 : 0 and C18 : 1 ω9c.
34241591	6	2	theme	gene	594:597	arg1	sequences					599:607	16S rRNA gene sequences	585:607	16S rRNA gene sequences	585:607	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Y6T belonged to the genus Nocardioides and showed the highest sequence similarity of 97.8 % to Nocardioides jishulii dk3136T.
34241591	7	3	theme	nucleotide	768:777	arg1	identity					779:786	average nucleotide identity	760:786	average nucleotide identity	760:786	The average nucleotide identity and in silico DNA-DNA hybridization values between strain Y6T and the reference strains were 76.9-81.2 % and 20.6-23.6 %, respectively.
34241591	7	4	dep	in	792:793	arg1	silico					795:800	silico	795:800	silico	795:800	The average nucleotide identity and in silico DNA-DNA hybridization values between strain Y6T and the reference strains were 76.9-81.2 % and 20.6-23.6 %, respectively.
34241591	8	5	theme	predominant	1013:1023	arg1	acids					1040:1044	the predominant cellular fatty acids	1009:1044	the predominant cellular fatty acids	1009:1044	Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-8(H4) and the predominant cellular fatty acids were iso-C16 : 0, 10-methyl-C17 : 0 and C18 : 1 ω9c.
34241591	8	5	theme	predominant	1013:1023	arg1	iso-C16 					1051:1058	iso-C16 	1051:1058	iso-C16 	1051:1058	Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-8(H4) and the predominant cellular fatty acids were iso-C16 : 0, 10-methyl-C17 : 0 and C18 : 1 ω9c.
34241591	11	6	theme	name	1530:1533	arg1	sp					1557:1558	the name Nocardioides malaquae sp	1526:1558	the name Nocardioides malaquae sp	1526:1558	On the basis of the phenotypic, genotypic, phylogenetic and chemotaxonomic features, strain Y6T is considered to represent a novel species, for which the name Nocardioides malaquae sp.
34241591	10	7	theme	ll-2,6-diaminopimelic	1348:1368	arg1	peptidoglycan					1330:1342	The peptidoglycan	1326:1342	The peptidoglycan	1326:1342	The peptidoglycan was ll-2,6-diaminopimelic acid.
34241591	10	7	theme	ll-2,6-diaminopimelic	1348:1368	arg1	acid					1370:1373	ll-2,6-diaminopimelic acid	1348:1373	ll-2,6-diaminopimelic acid	1348:1373	The peptidoglycan was ll-2,6-diaminopimelic acid.
34241591	6	8	theme	sequence	692:699	arg1	similarity					701:710	the highest sequence similarity	680:710	the highest sequence similarity of 97.8 % to Nocardioides jishulii dk3136T	680:753	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Y6T belonged to the genus Nocardioides and showed the highest sequence similarity of 97.8 % to Nocardioides jishulii dk3136T.
34241591	13	9	theme	=KCTC	1603:1607	arg1	Y6T					1598:1600	Y6T	1598:1600	Y6T (=KCTC 49504T=MCCC 1K04765T)	1598:1629	The type strain is Y6T (=KCTC 49504T=MCCC 1K04765T).
34241591	13	9	theme	=KCTC	1603:1607	arg1	1K04765T					1621:1628	=KCTC 49504T=MCCC 1K04765T	1603:1628	=KCTC 49504T=MCCC 1K04765T	1603:1628	The type strain is Y6T (=KCTC 49504T=MCCC 1K04765T).
34241591	11	10	theme	genotypic	1408:1416	arg1	features					1451:1458	the phenotypic, genotypic, phylogenetic and chemotaxonomic features	1392:1458	the phenotypic, genotypic, phylogenetic and chemotaxonomic features	1392:1458	On the basis of the phenotypic, genotypic, phylogenetic and chemotaxonomic features, strain Y6T is considered to represent a novel species, for which the name Nocardioides malaquae sp.
34241591	3	11	theme	growth	327:332	arg1	%					372:372	0-6.0 %	366:372	0-6.0 % (w/v)	366:378	The growth range of NaCl concentration was 0-6.0 % (w/v), with an optimum at 3.0 % (w/v).
34241591	3	11	theme	growth	327:332	arg1	range					334:338	The growth range	323:338	The growth range of NaCl concentration	323:360	The growth range of NaCl concentration was 0-6.0 % (w/v), with an optimum at 3.0 % (w/v).
34241591	11	12	theme	Nocardioides	1535:1546	arg1	sp					1557:1558	the name Nocardioides malaquae sp	1526:1558	the name Nocardioides malaquae sp	1526:1558	On the basis of the phenotypic, genotypic, phylogenetic and chemotaxonomic features, strain Y6T is considered to represent a novel species, for which the name Nocardioides malaquae sp.
34241591	8	13	theme	Chemotaxonomic	924:937	arg1	analysis					939:946	Chemotaxonomic analysis	924:946	Chemotaxonomic analysis	924:946	Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-8(H4) and the predominant cellular fatty acids were iso-C16 : 0, 10-methyl-C17 : 0 and C18 : 1 ω9c.
34241591	6	14	theme	highest	684:690	arg1	similarity					701:710	the highest sequence similarity	680:710	the highest sequence similarity of 97.8 % to Nocardioides jishulii dk3136T	680:753	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Y6T belonged to the genus Nocardioides and showed the highest sequence similarity of 97.8 % to Nocardioides jishulii dk3136T.
34241591	7	15	theme	DNA-DNA	802:808	arg1	hybridization					810:822	in silico DNA-DNA hybridization	792:822	in silico DNA-DNA hybridization	792:822	The average nucleotide identity and in silico DNA-DNA hybridization values between strain Y6T and the reference strains were 76.9-81.2 % and 20.6-23.6 %, respectively.
34241591	2	16	attach	isolated	198:205	arg1	sample					228:233	a sewage sludge sample	212:233	a sewage sludge sample collected from a fisheries processing factory in Zhoushan, Zhejiang Province, PR China	212:320	A Gram-stain-positive, rod-shaped, strictly aerobic bacterial strain (Y6T) was isolated from a sewage sludge sample collected from a fisheries processing factory in Zhoushan, Zhejiang Province, PR China.
34241591	2	16	attach	isolated	198:205	arg2	Y6T					189:191	Y6T	189:191	Y6T	189:191	A Gram-stain-positive, rod-shaped, strictly aerobic bacterial strain (Y6T) was isolated from a sewage sludge sample collected from a fisheries processing factory in Zhoushan, Zhejiang Province, PR China.
34241591	2	16	attach	isolated	198:205	arg2	strain					181:186	A Gram-stain-positive, rod-shaped, strictly aerobic bacterial strain	119:186	A Gram-stain-positive, rod-shaped, strictly aerobic bacterial strain (Y6T)	119:192	A Gram-stain-positive, rod-shaped, strictly aerobic bacterial strain (Y6T) was isolated from a sewage sludge sample collected from a fisheries processing factory in Zhoushan, Zhejiang Province, PR China.
34241591	8	17	theme	fatty	1034:1038	arg1	acids					1040:1044	the predominant cellular fatty acids	1009:1044	the predominant cellular fatty acids	1009:1044	Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-8(H4) and the predominant cellular fatty acids were iso-C16 : 0, 10-methyl-C17 : 0 and C18 : 1 ω9c.
34241591	8	17	theme	fatty	1034:1038	arg1	iso-C16 					1051:1058	iso-C16 	1051:1058	iso-C16 	1051:1058	Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-8(H4) and the predominant cellular fatty acids were iso-C16 : 0, 10-methyl-C17 : 0 and C18 : 1 ω9c.
34241591	3	18	with	%	372:372	arg1	optimum					389:395	an optimum	386:395	an optimum at 3.0 % (w/v)	386:410	The growth range of NaCl concentration was 0-6.0 % (w/v), with an optimum at 3.0 % (w/v).
34241591	7	19	theme	strain	839:844	arg1	Y6T					846:848	strain Y6T	839:848	strain Y6T	839:848	The average nucleotide identity and in silico DNA-DNA hybridization values between strain Y6T and the reference strains were 76.9-81.2 % and 20.6-23.6 %, respectively.
34241591	2	20	theme	processing	262:271	arg1	factory					273:279	a fisheries processing factory	250:279	a fisheries processing factory in Zhoushan, Zhejiang Province, PR China	250:320	A Gram-stain-positive, rod-shaped, strictly aerobic bacterial strain (Y6T) was isolated from a sewage sludge sample collected from a fisheries processing factory in Zhoushan, Zhejiang Province, PR China.
34241591	6	21	theme	rRNA	589:592	arg1	sequences					599:607	16S rRNA gene sequences	585:607	16S rRNA gene sequences	585:607	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Y6T belonged to the genus Nocardioides and showed the highest sequence similarity of 97.8 % to Nocardioides jishulii dk3136T.
34241591	5	22	theme	pH	514:515	arg1	7.0-10.0					517:524	pH 7.0-10.0	514:524	pH 7.0-10.0	514:524	The pH range for growth was pH 7.0-10.0, with an optimum at pH 9.0.
34241591	5	22	theme	pH	514:515	arg1	range					493:497	The pH range	486:497	The pH range for growth	486:508	The pH range for growth was pH 7.0-10.0, with an optimum at pH 9.0.
34241591	9	23	theme	unidentified	1237:1248	arg1	phospholipids					1250:1262	four unidentified phospholipids	1232:1262	four unidentified phospholipids	1232:1262	The polar lipid profile was composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, four unidentified phospholipids, three unidentified aminolipids and five unidentified lipids.
34241591	2	24	theme	fisheries	252:260	arg1	factory					273:279	a fisheries processing factory	250:279	a fisheries processing factory in Zhoushan, Zhejiang Province, PR China	250:320	A Gram-stain-positive, rod-shaped, strictly aerobic bacterial strain (Y6T) was isolated from a sewage sludge sample collected from a fisheries processing factory in Zhoushan, Zhejiang Province, PR China.
34241591	6	25	theme	16S	585:587	arg1	sequences					599:607	16S rRNA gene sequences	585:607	16S rRNA gene sequences	585:607	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Y6T belonged to the genus Nocardioides and showed the highest sequence similarity of 97.8 % to Nocardioides jishulii dk3136T.
34241591	4	26	from	37 °C	479:483	arg1	optimum					468:474	an optimum	465:474	an optimum at 37 °C	465:483	The temperature range for growth was 10-42 °C, with an optimum at 37 °C.
34241591	9	27	theme	unidentified	1305:1316	arg1	lipids					1318:1323	five unidentified lipids	1300:1323	five unidentified lipids	1300:1323	The polar lipid profile was composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, four unidentified phospholipids, three unidentified aminolipids and five unidentified lipids.
34241591	1	28	theme	sewage	70:75	arg1	sludge					77:82	sewage sludge	70:82	sewage sludge of a fisheries processing factory	70:116	nov., a novel actinobacterium isolated from sewage sludge of a fisheries processing factory.
34241591	0	29	theme	malaquae	13:20	arg1	sp					22:23	Nocardioides malaquae sp	0:23	Nocardioides malaquae sp.	0:24	Nocardioides malaquae sp.
34241591	2	30	theme	Gram-stain-positive	121:139	arg1	Y6T					189:191	Y6T	189:191	Y6T	189:191	A Gram-stain-positive, rod-shaped, strictly aerobic bacterial strain (Y6T) was isolated from a sewage sludge sample collected from a fisheries processing factory in Zhoushan, Zhejiang Province, PR China.
34241591	2	30	theme	Gram-stain-positive	121:139	arg1	strain					181:186	A Gram-stain-positive, rod-shaped, strictly aerobic bacterial strain	119:186	A Gram-stain-positive, rod-shaped, strictly aerobic bacterial strain (Y6T)	119:192	A Gram-stain-positive, rod-shaped, strictly aerobic bacterial strain (Y6T) was isolated from a sewage sludge sample collected from a fisheries processing factory in Zhoushan, Zhejiang Province, PR China.
34241591	13	31	theme	type	1583:1586	arg1	Y6T					1598:1600	Y6T	1598:1600	Y6T (=KCTC 49504T=MCCC 1K04765T)	1598:1629	The type strain is Y6T (=KCTC 49504T=MCCC 1K04765T).
34241591	13	31	theme	type	1583:1586	arg1	strain					1588:1593	The type strain	1579:1593	The type strain	1579:1593	The type strain is Y6T (=KCTC 49504T=MCCC 1K04765T).
34241591	7	32	theme	hybridization	810:822	arg1	values					824:829	The average nucleotide identity and in silico DNA-DNA hybridization values	756:829	The average nucleotide identity and in silico DNA-DNA hybridization values between strain Y6T and the reference strains	756:874	The average nucleotide identity and in silico DNA-DNA hybridization values between strain Y6T and the reference strains were 76.9-81.2 % and 20.6-23.6 %, respectively.
34241591	7	32	theme	hybridization	810:822	arg1	%					907:907	76.9-81.2 % and 20.6-23.6 %	881:907	76.9-81.2 % and 20.6-23.6 %	881:907	The average nucleotide identity and in silico DNA-DNA hybridization values between strain Y6T and the reference strains were 76.9-81.2 % and 20.6-23.6 %, respectively.
34241591	11	33	theme	phenotypic	1396:1405	arg1	features					1451:1458	the phenotypic, genotypic, phylogenetic and chemotaxonomic features	1392:1458	the phenotypic, genotypic, phylogenetic and chemotaxonomic features	1392:1458	On the basis of the phenotypic, genotypic, phylogenetic and chemotaxonomic features, strain Y6T is considered to represent a novel species, for which the name Nocardioides malaquae sp.
34241591	0	34	theme	Nocardioides	0:11	arg1	sp					22:23	Nocardioides malaquae sp	0:23	Nocardioides malaquae sp.	0:24	Nocardioides malaquae sp.
34241591	1	35	attach	isolated	56:63	arg2	actinobacterium					40:54	a novel actinobacterium	32:54	a novel actinobacterium isolated from sewage sludge of a fisheries processing factory	32:116	nov., a novel actinobacterium isolated from sewage sludge of a fisheries processing factory.
34241591	1	35	attach	isolated	56:63	arg1	sludge					77:82	sewage sludge	70:82	sewage sludge of a fisheries processing factory	70:116	nov., a novel actinobacterium isolated from sewage sludge of a fisheries processing factory.
34241591	6	36	theme	genus	650:654	arg1	Nocardioides					656:667	the genus Nocardioides	646:667	the genus Nocardioides	646:667	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Y6T belonged to the genus Nocardioides and showed the highest sequence similarity of 97.8 % to Nocardioides jishulii dk3136T.
34241591	2	37	dep	Province	303:310	arg1	PR					313:314	PR	313:314	PR	313:314	A Gram-stain-positive, rod-shaped, strictly aerobic bacterial strain (Y6T) was isolated from a sewage sludge sample collected from a fisheries processing factory in Zhoushan, Zhejiang Province, PR China.
34241591	11	38	theme	phylogenetic	1419:1430	arg1	features					1451:1458	the phenotypic, genotypic, phylogenetic and chemotaxonomic features	1392:1458	the phenotypic, genotypic, phylogenetic and chemotaxonomic features	1392:1458	On the basis of the phenotypic, genotypic, phylogenetic and chemotaxonomic features, strain Y6T is considered to represent a novel species, for which the name Nocardioides malaquae sp.
34241591	2	39	dep	Gram-stain-positive	121:139	arg1	rod-shaped					142:151	rod-shaped	142:151	rod-shaped	142:151	A Gram-stain-positive, rod-shaped, strictly aerobic bacterial strain (Y6T) was isolated from a sewage sludge sample collected from a fisheries processing factory in Zhoushan, Zhejiang Province, PR China.
34241591	2	39	dep	Gram-stain-positive	121:139	arg1	aerobic					163:169	aerobic	163:169	aerobic	163:169	A Gram-stain-positive, rod-shaped, strictly aerobic bacterial strain (Y6T) was isolated from a sewage sludge sample collected from a fisheries processing factory in Zhoushan, Zhejiang Province, PR China.
34241591	8	40	theme	respiratory	972:982	arg1	quinone					984:990	the sole respiratory quinone	963:990	the sole respiratory quinone	963:990	Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-8(H4) and the predominant cellular fatty acids were iso-C16 : 0, 10-methyl-C17 : 0 and C18 : 1 ω9c.
34241591	8	40	theme	respiratory	972:982	arg1	MK-8					996:999	MK-8	996:999	MK-8	996:999	Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-8(H4) and the predominant cellular fatty acids were iso-C16 : 0, 10-methyl-C17 : 0 and C18 : 1 ω9c.
34241591	5	41	theme	pH	490:491	arg1	7.0-10.0					517:524	pH 7.0-10.0	514:524	pH 7.0-10.0	514:524	The pH range for growth was pH 7.0-10.0, with an optimum at pH 9.0.
34241591	5	41	theme	pH	490:491	arg1	range					493:497	The pH range	486:497	The pH range for growth	486:508	The pH range for growth was pH 7.0-10.0, with an optimum at pH 9.0.
34241591	6	42	theme	Phylogenetic	554:565	arg1	analysis					567:574	Phylogenetic analysis	554:574	Phylogenetic analysis based on 16S rRNA gene sequences	554:607	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Y6T belonged to the genus Nocardioides and showed the highest sequence similarity of 97.8 % to Nocardioides jishulii dk3136T.
34241591	3	43	theme	NaCl	343:346	arg1	concentration					348:360	NaCl concentration	343:360	NaCl concentration	343:360	The growth range of NaCl concentration was 0-6.0 % (w/v), with an optimum at 3.0 % (w/v).
34241591	8	44	theme	sole	967:970	arg1	quinone					984:990	the sole respiratory quinone	963:990	the sole respiratory quinone	963:990	Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-8(H4) and the predominant cellular fatty acids were iso-C16 : 0, 10-methyl-C17 : 0 and C18 : 1 ω9c.
34241591	8	44	theme	sole	967:970	arg1	MK-8					996:999	MK-8	996:999	MK-8	996:999	Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-8(H4) and the predominant cellular fatty acids were iso-C16 : 0, 10-methyl-C17 : 0 and C18 : 1 ω9c.
34241591	1	45	theme	fisheries	89:97	arg1	factory					110:116	a fisheries processing factory	87:116	a fisheries processing factory	87:116	nov., a novel actinobacterium isolated from sewage sludge of a fisheries processing factory.
34241591	13	46	theme	49504T=MCCC	1609:1619	arg1	Y6T					1598:1600	Y6T	1598:1600	Y6T (=KCTC 49504T=MCCC 1K04765T)	1598:1629	The type strain is Y6T (=KCTC 49504T=MCCC 1K04765T).
34241591	13	46	theme	49504T=MCCC	1609:1619	arg1	1K04765T					1621:1628	=KCTC 49504T=MCCC 1K04765T	1603:1628	=KCTC 49504T=MCCC 1K04765T	1603:1628	The type strain is Y6T (=KCTC 49504T=MCCC 1K04765T).
34241591	2	47	theme	bacterial	171:179	arg1	Y6T					189:191	Y6T	189:191	Y6T	189:191	A Gram-stain-positive, rod-shaped, strictly aerobic bacterial strain (Y6T) was isolated from a sewage sludge sample collected from a fisheries processing factory in Zhoushan, Zhejiang Province, PR China.
34241591	2	47	theme	bacterial	171:179	arg1	strain					181:186	A Gram-stain-positive, rod-shaped, strictly aerobic bacterial strain	119:186	A Gram-stain-positive, rod-shaped, strictly aerobic bacterial strain (Y6T)	119:192	A Gram-stain-positive, rod-shaped, strictly aerobic bacterial strain (Y6T) was isolated from a sewage sludge sample collected from a fisheries processing factory in Zhoushan, Zhejiang Province, PR China.
34241591	2	48	theme	sewage	214:219	arg1	sample					228:233	a sewage sludge sample	212:233	a sewage sludge sample collected from a fisheries processing factory in Zhoushan, Zhejiang Province, PR China	212:320	A Gram-stain-positive, rod-shaped, strictly aerobic bacterial strain (Y6T) was isolated from a sewage sludge sample collected from a fisheries processing factory in Zhoushan, Zhejiang Province, PR China.
34241591	11	49	theme	strain	1461:1466	arg1	Y6T					1468:1470	strain Y6T	1461:1470	strain Y6T	1461:1470	On the basis of the phenotypic, genotypic, phylogenetic and chemotaxonomic features, strain Y6T is considered to represent a novel species, for which the name Nocardioides malaquae sp.
34241591	7	50	theme	in	792:793	arg1	hybridization					810:822	in silico DNA-DNA hybridization	792:822	in silico DNA-DNA hybridization	792:822	The average nucleotide identity and in silico DNA-DNA hybridization values between strain Y6T and the reference strains were 76.9-81.2 % and 20.6-23.6 %, respectively.
34241591	6	51	dep	Nocardioides	725:736	arg1	jishulii					738:745	jishulii	738:745	jishulii	738:745	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Y6T belonged to the genus Nocardioides and showed the highest sequence similarity of 97.8 % to Nocardioides jishulii dk3136T.
34241591	6	52	theme	Nocardioides	725:736	arg1	dk3136T					747:753	Nocardioides jishulii dk3136T	725:753	Nocardioides jishulii dk3136T	725:753	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Y6T belonged to the genus Nocardioides and showed the highest sequence similarity of 97.8 % to Nocardioides jishulii dk3136T.
34241591	11	53	theme	novel	1501:1505	arg1	species					1507:1513	a novel species	1499:1513	a novel species	1499:1513	On the basis of the phenotypic, genotypic, phylogenetic and chemotaxonomic features, strain Y6T is considered to represent a novel species, for which the name Nocardioides malaquae sp.
34241591	5	54	from	pH	546:547	arg1	optimum					535:541	an optimum	532:541	an optimum at pH 9.0	532:551	The pH range for growth was pH 7.0-10.0, with an optimum at pH 9.0.
34241591	1	55	theme	processing	99:108	arg1	factory					110:116	a fisheries processing factory	87:116	a fisheries processing factory	87:116	nov., a novel actinobacterium isolated from sewage sludge of a fisheries processing factory.
34241591	11	56	theme	malaquae	1548:1555	arg1	sp					1557:1558	the name Nocardioides malaquae sp	1526:1558	the name Nocardioides malaquae sp	1526:1558	On the basis of the phenotypic, genotypic, phylogenetic and chemotaxonomic features, strain Y6T is considered to represent a novel species, for which the name Nocardioides malaquae sp.
34241591	6	57	theme	%	720:720	arg1	similarity					701:710	the highest sequence similarity	680:710	the highest sequence similarity of 97.8 % to Nocardioides jishulii dk3136T	680:753	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Y6T belonged to the genus Nocardioides and showed the highest sequence similarity of 97.8 % to Nocardioides jishulii dk3136T.
34241591	7	58	theme	%	891:891	arg1	values					824:829	The average nucleotide identity and in silico DNA-DNA hybridization values	756:829	The average nucleotide identity and in silico DNA-DNA hybridization values between strain Y6T and the reference strains	756:874	The average nucleotide identity and in silico DNA-DNA hybridization values between strain Y6T and the reference strains were 76.9-81.2 % and 20.6-23.6 %, respectively.
34241591	7	58	theme	%	891:891	arg1	%					907:907	76.9-81.2 % and 20.6-23.6 %	881:907	76.9-81.2 % and 20.6-23.6 %	881:907	The average nucleotide identity and in silico DNA-DNA hybridization values between strain Y6T and the reference strains were 76.9-81.2 % and 20.6-23.6 %, respectively.
34241591	1	59	theme	factory	110:116	arg1	sludge					77:82	sewage sludge	70:82	sewage sludge of a fisheries processing factory	70:116	nov., a novel actinobacterium isolated from sewage sludge of a fisheries processing factory.
34241591	7	60	theme	identity	779:786	arg1	values					824:829	The average nucleotide identity and in silico DNA-DNA hybridization values	756:829	The average nucleotide identity and in silico DNA-DNA hybridization values between strain Y6T and the reference strains	756:874	The average nucleotide identity and in silico DNA-DNA hybridization values between strain Y6T and the reference strains were 76.9-81.2 % and 20.6-23.6 %, respectively.
34241591	7	60	theme	identity	779:786	arg1	%					907:907	76.9-81.2 % and 20.6-23.6 %	881:907	76.9-81.2 % and 20.6-23.6 %	881:907	The average nucleotide identity and in silico DNA-DNA hybridization values between strain Y6T and the reference strains were 76.9-81.2 % and 20.6-23.6 %, respectively.
34241591	1	61	theme	novel	34:38	arg1	actinobacterium					40:54	a novel actinobacterium	32:54	a novel actinobacterium isolated from sewage sludge of a fisheries processing factory	32:116	nov., a novel actinobacterium isolated from sewage sludge of a fisheries processing factory.
34241591	9	62	theme	polar	1103:1107	arg1	profile					1115:1121	The polar lipid profile	1099:1121	The polar lipid profile	1099:1121	The polar lipid profile was composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, four unidentified phospholipids, three unidentified aminolipids and five unidentified lipids.
34241591	11	63	theme	chemotaxonomic	1436:1449	arg1	features					1451:1458	the phenotypic, genotypic, phylogenetic and chemotaxonomic features	1392:1458	the phenotypic, genotypic, phylogenetic and chemotaxonomic features	1392:1458	On the basis of the phenotypic, genotypic, phylogenetic and chemotaxonomic features, strain Y6T is considered to represent a novel species, for which the name Nocardioides malaquae sp.
34241591	3	64	from	%	404:404	arg1	optimum					389:395	an optimum	386:395	an optimum at 3.0 % (w/v)	386:410	The growth range of NaCl concentration was 0-6.0 % (w/v), with an optimum at 3.0 % (w/v).
34241591	1	65	dep	actinobacterium	40:54	arg1	nov.					26:29	nov.	26:29	nov.	26:29	nov., a novel actinobacterium isolated from sewage sludge of a fisheries processing factory.
34241591	11	66	dep	features	1451:1458	arg1	the					1379:1381	the	1379:1381	the	1379:1381	On the basis of the phenotypic, genotypic, phylogenetic and chemotaxonomic features, strain Y6T is considered to represent a novel species, for which the name Nocardioides malaquae sp.
34241591	11	66	dep	features	1451:1458	arg1	basis					1383:1387	basis	1383:1387	basis	1383:1387	On the basis of the phenotypic, genotypic, phylogenetic and chemotaxonomic features, strain Y6T is considered to represent a novel species, for which the name Nocardioides malaquae sp.
34241591	9	67	theme	unidentified	1271:1282	arg1	aminolipids					1284:1294	three unidentified aminolipids	1265:1294	three unidentified aminolipids	1265:1294	The polar lipid profile was composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, four unidentified phospholipids, three unidentified aminolipids and five unidentified lipids.
34241591	6	68	theme	strain	623:628	arg1	Y6T					630:632	strain Y6T	623:632	strain Y6T	623:632	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Y6T belonged to the genus Nocardioides and showed the highest sequence similarity of 97.8 % to Nocardioides jishulii dk3136T.
34241591	9	69	theme	lipid	1109:1113	arg1	profile					1115:1121	The polar lipid profile	1099:1121	The polar lipid profile	1099:1121	The polar lipid profile was composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, four unidentified phospholipids, three unidentified aminolipids and five unidentified lipids.
34241591	5	70	with	7.0-10.0	517:524	arg1	optimum					535:541	an optimum	532:541	an optimum at pH 9.0	532:551	The pH range for growth was pH 7.0-10.0, with an optimum at pH 9.0.
34241591	2	71	from	factory	273:279	arg1	China					316:320	China	316:320	China	316:320	A Gram-stain-positive, rod-shaped, strictly aerobic bacterial strain (Y6T) was isolated from a sewage sludge sample collected from a fisheries processing factory in Zhoushan, Zhejiang Province, PR China.
34241591	7	72	theme	 and	892:895	arg1	values					824:829	The average nucleotide identity and in silico DNA-DNA hybridization values	756:829	The average nucleotide identity and in silico DNA-DNA hybridization values between strain Y6T and the reference strains	756:874	The average nucleotide identity and in silico DNA-DNA hybridization values between strain Y6T and the reference strains were 76.9-81.2 % and 20.6-23.6 %, respectively.
34241591	7	72	theme	 and	892:895	arg1	%					907:907	76.9-81.2 % and 20.6-23.6 %	881:907	76.9-81.2 % and 20.6-23.6 %	881:907	The average nucleotide identity and in silico DNA-DNA hybridization values between strain Y6T and the reference strains were 76.9-81.2 % and 20.6-23.6 %, respectively.
34241591	7	73	theme	76.9-81.2 	881:890	arg1	values					824:829	The average nucleotide identity and in silico DNA-DNA hybridization values	756:829	The average nucleotide identity and in silico DNA-DNA hybridization values between strain Y6T and the reference strains	756:874	The average nucleotide identity and in silico DNA-DNA hybridization values between strain Y6T and the reference strains were 76.9-81.2 % and 20.6-23.6 %, respectively.
34241591	7	73	theme	76.9-81.2 	881:890	arg1	%					907:907	76.9-81.2 % and 20.6-23.6 %	881:907	76.9-81.2 % and 20.6-23.6 %	881:907	The average nucleotide identity and in silico DNA-DNA hybridization values between strain Y6T and the reference strains were 76.9-81.2 % and 20.6-23.6 %, respectively.
34241591	4	74	theme	temperature	417:427	arg1	range					429:433	The temperature range	413:433	The temperature range for growth	413:444	The temperature range for growth was 10-42 °C, with an optimum at 37 °C.
34241591	2	75	theme	sludge	221:226	arg1	sample					228:233	a sewage sludge sample	212:233	a sewage sludge sample collected from a fisheries processing factory in Zhoushan, Zhejiang Province, PR China	212:320	A Gram-stain-positive, rod-shaped, strictly aerobic bacterial strain (Y6T) was isolated from a sewage sludge sample collected from a fisheries processing factory in Zhoushan, Zhejiang Province, PR China.
34241591	7	76	theme	reference	858:866	arg1	strains					868:874	the reference strains	854:874	the reference strains	854:874	The average nucleotide identity and in silico DNA-DNA hybridization values between strain Y6T and the reference strains were 76.9-81.2 % and 20.6-23.6 %, respectively.
34241591	3	77	theme	concentration	348:360	arg1	%					372:372	0-6.0 %	366:372	0-6.0 % (w/v)	366:378	The growth range of NaCl concentration was 0-6.0 % (w/v), with an optimum at 3.0 % (w/v).
34241591	3	77	theme	concentration	348:360	arg1	range					334:338	The growth range	323:338	The growth range of NaCl concentration	323:360	The growth range of NaCl concentration was 0-6.0 % (w/v), with an optimum at 3.0 % (w/v).
34241591	7	78	theme	average	760:766	arg1	identity					779:786	average nucleotide identity	760:786	average nucleotide identity	760:786	The average nucleotide identity and in silico DNA-DNA hybridization values between strain Y6T and the reference strains were 76.9-81.2 % and 20.6-23.6 %, respectively.
31951100	7	0	theme	prebiotic	1184:1192	arg1	effect					1194:1199	a prebiotic effect	1182:1199	a prebiotic effect on gut microbiota	1182:1217	Moreover, TEs are capable of exerting a prebiotic effect on gut microbiota by increasing the abundance of potentially beneficial bacteria (e.g., Faecalibaculum, and Bifidobacterium), and decreasing the abundance of potentially harmful bacteria (e.g., Bacteroids, and Mucispirillum).
31951100	2	1	theme	oolong	376:381	arg1	tea					400:402	white, yellow, oolong, black, and dark tea	361:402	white, yellow, oolong, black, and dark tea	361:402	The in vivo and in vitro anti-inflammatory effects of Camellia sinensis (green, white, yellow, oolong, black, and dark tea) and Litsea coreana (hawk tea) are comparatively explored.
31951100	0	2	theme	Gut	85:87	arg1	Microbiota					89:98	Gut Microbiota	85:98	Gut Microbiota	85:98	Camellia sinensis and Litsea coreana Ameliorate Intestinal Inflammation and Modulate Gut Microbiota in Dextran Sulfate Sodium-Induced Colitis Mice.
31951100	4	3	from	model	854:858	arg1	macrophages					785:795	LPS-stimulated RAW 264.7 macrophages	760:795	LPS-stimulated RAW 264.7 macrophages	760:795	The tea extracts (TEs) significantly decrease the production of pro-inflammatory cytokines (IL-6, IL-12, and tumor necrosis factor-α) and increase the anti-inflammatory cytokines (IL-10) in LPS-stimulated RAW 264.7 macrophages and a dextran sodium sulfate (DSS)-induced colitis mouse model.
31951100	4	4	theme	mouse	848:852	arg1	model					854:858	a dextran sodium sulfate (DSS)-induced colitis mouse model	801:858	a dextran sodium sulfate (DSS)-induced colitis mouse model	801:858	The tea extracts (TEs) significantly decrease the production of pro-inflammatory cytokines (IL-6, IL-12, and tumor necrosis factor-α) and increase the anti-inflammatory cytokines (IL-10) in LPS-stimulated RAW 264.7 macrophages and a dextran sodium sulfate (DSS)-induced colitis mouse model.
31951100	4	5	theme	necrosis	685:692	arg1	cytokines					651:659	pro-inflammatory cytokines	634:659	pro-inflammatory cytokines (IL-6, IL-12, and tumor necrosis factor-α)	634:702	The tea extracts (TEs) significantly decrease the production of pro-inflammatory cytokines (IL-6, IL-12, and tumor necrosis factor-α) and increase the anti-inflammatory cytokines (IL-10) in LPS-stimulated RAW 264.7 macrophages and a dextran sodium sulfate (DSS)-induced colitis mouse model.
31951100	4	5	theme	necrosis	685:692	arg1	factor-α					694:701	tumor necrosis factor-α	679:701	tumor necrosis factor-α	679:701	The tea extracts (TEs) significantly decrease the production of pro-inflammatory cytokines (IL-6, IL-12, and tumor necrosis factor-α) and increase the anti-inflammatory cytokines (IL-10) in LPS-stimulated RAW 264.7 macrophages and a dextran sodium sulfate (DSS)-induced colitis mouse model.
31951100	5	6	theme	colon	964:968	arg1	integrity					970:978	colon integrity	964:978	colon integrity	964:978	The treatment of TEs in colitis mice can ameliorate colon inflammation, pro-oxidative enzyme activity, colon integrity, and suppress the activation of nuclear factor-κB.
31951100	5	6	theme	colon	964:968	arg1	inflammation					919:930	colon inflammation	913:930	colon inflammation	913:930	The treatment of TEs in colitis mice can ameliorate colon inflammation, pro-oxidative enzyme activity, colon integrity, and suppress the activation of nuclear factor-κB.
31951100	0	7	theme	Dextran	103:109	arg1	Sulfate					111:117	Dextran Sulfate	103:117	Dextran Sulfate Sodium-Induced Colitis Mice	103:145	Camellia sinensis and Litsea coreana Ameliorate Intestinal Inflammation and Modulate Gut Microbiota in Dextran Sulfate Sodium-Induced Colitis Mice.
31951100	9	8	theme	microbiota	1627:1636	arg1	dysbiosis					1610:1618	the dysbiosis	1606:1618	the dysbiosis of gut microbiota in colitis mice	1606:1652	CONCLUSION The treatment of seven types of tea can alleviate DSS-induced colitis in mice, and modulate the dysbiosis of gut microbiota in colitis mice.
31951100	5	9	theme	enzyme	947:952	arg1	activity					954:961	pro-oxidative enzyme activity	933:961	pro-oxidative enzyme activity	933:961	The treatment of TEs in colitis mice can ameliorate colon inflammation, pro-oxidative enzyme activity, colon integrity, and suppress the activation of nuclear factor-κB.
31951100	5	9	theme	enzyme	947:952	arg1	inflammation					919:930	colon inflammation	913:930	colon inflammation	913:930	The treatment of TEs in colitis mice can ameliorate colon inflammation, pro-oxidative enzyme activity, colon integrity, and suppress the activation of nuclear factor-κB.
31951100	7	10	theme	bacteria	1379:1386	arg1	abundance					1346:1354	the abundance	1342:1354	the abundance of potentially harmful bacteria (e.g., Bacteroids, and Mucispirillum)	1342:1424	Moreover, TEs are capable of exerting a prebiotic effect on gut microbiota by increasing the abundance of potentially beneficial bacteria (e.g., Faecalibaculum, and Bifidobacterium), and decreasing the abundance of potentially harmful bacteria (e.g., Bacteroids, and Mucispirillum).
31951100	5	11	theme	colon	913:917	arg1	activity					954:961	pro-oxidative enzyme activity	933:961	pro-oxidative enzyme activity	933:961	The treatment of TEs in colitis mice can ameliorate colon inflammation, pro-oxidative enzyme activity, colon integrity, and suppress the activation of nuclear factor-κB.
31951100	5	11	theme	colon	913:917	arg1	integrity					970:978	colon integrity	964:978	colon integrity	964:978	The treatment of TEs in colitis mice can ameliorate colon inflammation, pro-oxidative enzyme activity, colon integrity, and suppress the activation of nuclear factor-κB.
31951100	5	11	theme	colon	913:917	arg1	inflammation					919:930	colon inflammation	913:930	colon inflammation	913:930	The treatment of TEs in colitis mice can ameliorate colon inflammation, pro-oxidative enzyme activity, colon integrity, and suppress the activation of nuclear factor-κB.
31951100	5	12	from	treatment	865:873	arg1	mice					893:896	colitis mice	885:896	colitis mice	885:896	The treatment of TEs in colitis mice can ameliorate colon inflammation, pro-oxidative enzyme activity, colon integrity, and suppress the activation of nuclear factor-κB.
31951100	5	13	theme	TEs	878:880	arg1	treatment					865:873	The treatment	861:873	The treatment of TEs in colitis mice	861:896	The treatment of TEs in colitis mice can ameliorate colon inflammation, pro-oxidative enzyme activity, colon integrity, and suppress the activation of nuclear factor-κB.
31951100	9	14	dep	CONCLUSION	1503:1512	arg1	alleviate					1554:1562	alleviate	1554:1562	alleviate DSS-induced colitis in mice	1554:1590	CONCLUSION The treatment of seven types of tea can alleviate DSS-induced colitis in mice, and modulate the dysbiosis of gut microbiota in colitis mice.
31951100	9	14	dep	CONCLUSION	1503:1512	arg1	modulate					1597:1604	modulate	1597:1604	modulate the dysbiosis of gut microbiota in colitis mice	1597:1652	CONCLUSION The treatment of seven types of tea can alleviate DSS-induced colitis in mice, and modulate the dysbiosis of gut microbiota in colitis mice.
31951100	0	15	theme	Sodium-Induced	119:132	arg1	Colitis					134:140	Dextran Sulfate Sodium-Induced Colitis	103:140	Dextran Sulfate Sodium-Induced Colitis Mice	103:145	Camellia sinensis and Litsea coreana Ameliorate Intestinal Inflammation and Modulate Gut Microbiota in Dextran Sulfate Sodium-Induced Colitis Mice.
31951100	2	16	dep	sinensis	344:351	arg1	tea					400:402	white, yellow, oolong, black, and dark tea	361:402	white, yellow, oolong, black, and dark tea	361:402	The in vivo and in vitro anti-inflammatory effects of Camellia sinensis (green, white, yellow, oolong, black, and dark tea) and Litsea coreana (hawk tea) are comparatively explored.
31951100	2	17	theme	anti-inflammatory	306:322	arg1	effects					324:330	The in vivo and in vitro anti-inflammatory effects	281:330	The in vivo and in vitro anti-inflammatory effects of Camellia sinensis (green, white, yellow, oolong, black, and dark tea) and Litsea coreana (hawk tea)	281:433	The in vivo and in vitro anti-inflammatory effects of Camellia sinensis (green, white, yellow, oolong, black, and dark tea) and Litsea coreana (hawk tea) are comparatively explored.
31951100	0	18	theme	Sulfate	111:117	arg1	Colitis					134:140	Dextran Sulfate Sodium-Induced Colitis	103:140	Dextran Sulfate Sodium-Induced Colitis Mice	103:145	Camellia sinensis and Litsea coreana Ameliorate Intestinal Inflammation and Modulate Gut Microbiota in Dextran Sulfate Sodium-Induced Colitis Mice.
31951100	8	19	theme	mice	1497:1500	arg1	feces					1480:1484	the feces	1476:1484	the feces of colitic mice	1476:1500	TEs restore the decreased production of SCFAs in the feces of colitic mice.
31951100	9	20	theme	gut	1623:1625	arg1	microbiota					1627:1636	gut microbiota	1623:1636	gut microbiota	1623:1636	CONCLUSION The treatment of seven types of tea can alleviate DSS-induced colitis in mice, and modulate the dysbiosis of gut microbiota in colitis mice.
31951100	6	21	theme	gut	1128:1130	arg1	microbiota					1132:1141	gut microbiota	1128:1141	gut microbiota	1128:1141	Of note, green TE significantly attenuates the DSS-induced decrease in richness and diversity of gut microbiota.
31951100	4	22	theme	LPS-stimulated	760:773	arg1	macrophages					785:795	LPS-stimulated RAW 264.7 macrophages	760:795	LPS-stimulated RAW 264.7 macrophages	760:795	The tea extracts (TEs) significantly decrease the production of pro-inflammatory cytokines (IL-6, IL-12, and tumor necrosis factor-α) and increase the anti-inflammatory cytokines (IL-10) in LPS-stimulated RAW 264.7 macrophages and a dextran sodium sulfate (DSS)-induced colitis mouse model.
31951100	3	23	theme	RESULTS	475:481	arg1	analysis					488:495	METHODS AND RESULTS HPLC analysis	463:495	METHODS AND RESULTS HPLC analysis	463:495	METHODS AND RESULTS HPLC analysis confirms dissimilarities among phytochemical compositions of these teas.
31951100	2	24	theme	dark	395:398	arg1	tea					400:402	white, yellow, oolong, black, and dark tea	361:402	white, yellow, oolong, black, and dark tea	361:402	The in vivo and in vitro anti-inflammatory effects of Camellia sinensis (green, white, yellow, oolong, black, and dark tea) and Litsea coreana (hawk tea) are comparatively explored.
31951100	8	25	theme	colitic	1489:1495	arg1	mice					1497:1500	colitic mice	1489:1500	colitic mice	1489:1500	TEs restore the decreased production of SCFAs in the feces of colitic mice.
31951100	4	26	theme	colitis	840:846	arg1	model					854:858	a dextran sodium sulfate (DSS)-induced colitis mouse model	801:858	a dextran sodium sulfate (DSS)-induced colitis mouse model	801:858	The tea extracts (TEs) significantly decrease the production of pro-inflammatory cytokines (IL-6, IL-12, and tumor necrosis factor-α) and increase the anti-inflammatory cytokines (IL-10) in LPS-stimulated RAW 264.7 macrophages and a dextran sodium sulfate (DSS)-induced colitis mouse model.
31951100	2	27	theme	green	354:358	arg1	tea					400:402	white, yellow, oolong, black, and dark tea	361:402	white, yellow, oolong, black, and dark tea	361:402	The in vivo and in vitro anti-inflammatory effects of Camellia sinensis (green, white, yellow, oolong, black, and dark tea) and Litsea coreana (hawk tea) are comparatively explored.
31951100	3	28	theme	phytochemical	528:540	arg1	compositions					542:553	phytochemical compositions	528:553	phytochemical compositions of these teas	528:567	METHODS AND RESULTS HPLC analysis confirms dissimilarities among phytochemical compositions of these teas.
31951100	6	29	theme	green	1040:1044	arg1	TE					1046:1047	green TE	1040:1047	green TE	1040:1047	Of note, green TE significantly attenuates the DSS-induced decrease in richness and diversity of gut microbiota.
31951100	4	30	theme	-induced	831:838	arg1	model					854:858	a dextran sodium sulfate (DSS)-induced colitis mouse model	801:858	a dextran sodium sulfate (DSS)-induced colitis mouse model	801:858	The tea extracts (TEs) significantly decrease the production of pro-inflammatory cytokines (IL-6, IL-12, and tumor necrosis factor-α) and increase the anti-inflammatory cytokines (IL-10) in LPS-stimulated RAW 264.7 macrophages and a dextran sodium sulfate (DSS)-induced colitis mouse model.
31951100	4	31	from	cytokines	739:747	arg1	macrophages					785:795	LPS-stimulated RAW 264.7 macrophages	760:795	LPS-stimulated RAW 264.7 macrophages	760:795	The tea extracts (TEs) significantly decrease the production of pro-inflammatory cytokines (IL-6, IL-12, and tumor necrosis factor-α) and increase the anti-inflammatory cytokines (IL-10) in LPS-stimulated RAW 264.7 macrophages and a dextran sodium sulfate (DSS)-induced colitis mouse model.
31951100	4	32	theme	anti-inflammatory	721:737	arg1	IL-10					750:754	IL-10	750:754	IL-10	750:754	The tea extracts (TEs) significantly decrease the production of pro-inflammatory cytokines (IL-6, IL-12, and tumor necrosis factor-α) and increase the anti-inflammatory cytokines (IL-10) in LPS-stimulated RAW 264.7 macrophages and a dextran sodium sulfate (DSS)-induced colitis mouse model.
31951100	4	32	theme	anti-inflammatory	721:737	arg1	cytokines					739:747	the anti-inflammatory cytokines	717:747	the anti-inflammatory cytokines (IL-10) in LPS-stimulated RAW 264.7 macrophages	717:795	The tea extracts (TEs) significantly decrease the production of pro-inflammatory cytokines (IL-6, IL-12, and tumor necrosis factor-α) and increase the anti-inflammatory cytokines (IL-10) in LPS-stimulated RAW 264.7 macrophages and a dextran sodium sulfate (DSS)-induced colitis mouse model.
31951100	9	33	from	colitis	1576:1582	arg1	mice					1587:1590	mice	1587:1590	mice	1587:1590	CONCLUSION The treatment of seven types of tea can alleviate DSS-induced colitis in mice, and modulate the dysbiosis of gut microbiota in colitis mice.
31951100	1	34	theme	alternative	210:220	arg1	strategies					234:243	alternative therapeutic strategies	210:243	alternative therapeutic strategies for experimentally induced colitis	210:278	SCOPE Polyphenol-enriched herbal extracts have been proved as alternative therapeutic strategies for experimentally induced colitis.
31951100	2	35	theme	hawk	425:428	arg1	tea					430:432	hawk tea	425:432	hawk tea	425:432	The in vivo and in vitro anti-inflammatory effects of Camellia sinensis (green, white, yellow, oolong, black, and dark tea) and Litsea coreana (hawk tea) are comparatively explored.
31951100	2	35	theme	hawk	425:428	arg1	sinensis					344:351	Camellia sinensis	335:351	Camellia sinensis (green, white, yellow, oolong, black, and dark tea)	335:403	The in vivo and in vitro anti-inflammatory effects of Camellia sinensis (green, white, yellow, oolong, black, and dark tea) and Litsea coreana (hawk tea) are comparatively explored.
31951100	9	36	theme	colitis	1641:1647	arg1	mice					1649:1652	colitis mice	1641:1652	colitis mice	1641:1652	CONCLUSION The treatment of seven types of tea can alleviate DSS-induced colitis in mice, and modulate the dysbiosis of gut microbiota in colitis mice.
31951100	9	37	theme	DSS-induced	1564:1574	arg1	colitis					1576:1582	DSS-induced colitis	1564:1582	DSS-induced colitis in mice	1564:1590	CONCLUSION The treatment of seven types of tea can alleviate DSS-induced colitis in mice, and modulate the dysbiosis of gut microbiota in colitis mice.
31951100	2	38	theme	sinensis	344:351	arg1	effects					324:330	The in vivo and in vitro anti-inflammatory effects	281:330	The in vivo and in vitro anti-inflammatory effects of Camellia sinensis (green, white, yellow, oolong, black, and dark tea) and Litsea coreana (hawk tea)	281:433	The in vivo and in vitro anti-inflammatory effects of Camellia sinensis (green, white, yellow, oolong, black, and dark tea) and Litsea coreana (hawk tea) are comparatively explored.
31951100	2	39	theme	coreana	416:422	arg1	effects					324:330	The in vivo and in vitro anti-inflammatory effects	281:330	The in vivo and in vitro anti-inflammatory effects of Camellia sinensis (green, white, yellow, oolong, black, and dark tea) and Litsea coreana (hawk tea)	281:433	The in vivo and in vitro anti-inflammatory effects of Camellia sinensis (green, white, yellow, oolong, black, and dark tea) and Litsea coreana (hawk tea) are comparatively explored.
31951100	6	40	theme	microbiota	1132:1141	arg1	richness					1102:1109	richness	1102:1109	richness	1102:1109	Of note, green TE significantly attenuates the DSS-induced decrease in richness and diversity of gut microbiota.
31951100	6	40	theme	microbiota	1132:1141	arg1	diversity					1115:1123	diversity	1115:1123	diversity	1115:1123	Of note, green TE significantly attenuates the DSS-induced decrease in richness and diversity of gut microbiota.
31951100	4	41	theme	tea	574:576	arg1	TEs					588:590	TEs	588:590	TEs	588:590	The tea extracts (TEs) significantly decrease the production of pro-inflammatory cytokines (IL-6, IL-12, and tumor necrosis factor-α) and increase the anti-inflammatory cytokines (IL-10) in LPS-stimulated RAW 264.7 macrophages and a dextran sodium sulfate (DSS)-induced colitis mouse model.
31951100	4	41	theme	tea	574:576	arg1	extracts					578:585	The tea extracts	570:585	The tea extracts (TEs)	570:591	The tea extracts (TEs) significantly decrease the production of pro-inflammatory cytokines (IL-6, IL-12, and tumor necrosis factor-α) and increase the anti-inflammatory cytokines (IL-10) in LPS-stimulated RAW 264.7 macrophages and a dextran sodium sulfate (DSS)-induced colitis mouse model.
31951100	0	42	theme	Colitis	134:140	arg1	Mice					142:145	Dextran Sulfate Sodium-Induced Colitis Mice	103:145	Dextran Sulfate Sodium-Induced Colitis Mice	103:145	Camellia sinensis and Litsea coreana Ameliorate Intestinal Inflammation and Modulate Gut Microbiota in Dextran Sulfate Sodium-Induced Colitis Mice.
31951100	5	43	theme	pro-oxidative	933:945	arg1	activity					954:961	pro-oxidative enzyme activity	933:961	pro-oxidative enzyme activity	933:961	The treatment of TEs in colitis mice can ameliorate colon inflammation, pro-oxidative enzyme activity, colon integrity, and suppress the activation of nuclear factor-κB.
31951100	5	43	theme	pro-oxidative	933:945	arg1	inflammation					919:930	colon inflammation	913:930	colon inflammation	913:930	The treatment of TEs in colitis mice can ameliorate colon inflammation, pro-oxidative enzyme activity, colon integrity, and suppress the activation of nuclear factor-κB.
31951100	1	44	theme	Polyphenol-enriched	154:172	arg1	extracts					181:188	SCOPE Polyphenol-enriched herbal extracts	148:188	SCOPE Polyphenol-enriched herbal extracts	148:188	SCOPE Polyphenol-enriched herbal extracts have been proved as alternative therapeutic strategies for experimentally induced colitis.
31951100	7	45	theme	beneficial	1262:1271	arg1	bacteria					1273:1280	potentially beneficial bacteria	1250:1280	potentially beneficial bacteria (e.g., Faecalibaculum, and Bifidobacterium)	1250:1324	Moreover, TEs are capable of exerting a prebiotic effect on gut microbiota by increasing the abundance of potentially beneficial bacteria (e.g., Faecalibaculum, and Bifidobacterium), and decreasing the abundance of potentially harmful bacteria (e.g., Bacteroids, and Mucispirillum).
31951100	9	46	theme	types	1537:1541	arg1	treatment					1518:1526	The treatment	1514:1526	The treatment of seven types of tea	1514:1548	CONCLUSION The treatment of seven types of tea can alleviate DSS-induced colitis in mice, and modulate the dysbiosis of gut microbiota in colitis mice.
31951100	8	47	theme	decreased	1443:1451	arg1	production					1453:1462	the decreased production	1439:1462	the decreased production of SCFAs in the feces of colitic mice	1439:1500	TEs restore the decreased production of SCFAs in the feces of colitic mice.
31951100	9	48	theme	tea	1546:1548	arg1	types					1537:1541	seven types	1531:1541	seven types of tea	1531:1548	CONCLUSION The treatment of seven types of tea can alleviate DSS-induced colitis in mice, and modulate the dysbiosis of gut microbiota in colitis mice.
31951100	7	49	theme	bacteria	1273:1280	arg1	abundance					1237:1245	the abundance	1233:1245	the abundance of potentially beneficial bacteria (e.g., Faecalibaculum, and Bifidobacterium)	1233:1324	Moreover, TEs are capable of exerting a prebiotic effect on gut microbiota by increasing the abundance of potentially beneficial bacteria (e.g., Faecalibaculum, and Bifidobacterium), and decreasing the abundance of potentially harmful bacteria (e.g., Bacteroids, and Mucispirillum).
31951100	4	50	theme	tumor	679:683	arg1	cytokines					651:659	pro-inflammatory cytokines	634:659	pro-inflammatory cytokines (IL-6, IL-12, and tumor necrosis factor-α)	634:702	The tea extracts (TEs) significantly decrease the production of pro-inflammatory cytokines (IL-6, IL-12, and tumor necrosis factor-α) and increase the anti-inflammatory cytokines (IL-10) in LPS-stimulated RAW 264.7 macrophages and a dextran sodium sulfate (DSS)-induced colitis mouse model.
31951100	4	50	theme	tumor	679:683	arg1	factor-α					694:701	tumor necrosis factor-α	679:701	tumor necrosis factor-α	679:701	The tea extracts (TEs) significantly decrease the production of pro-inflammatory cytokines (IL-6, IL-12, and tumor necrosis factor-α) and increase the anti-inflammatory cytokines (IL-10) in LPS-stimulated RAW 264.7 macrophages and a dextran sodium sulfate (DSS)-induced colitis mouse model.
31951100	7	51	theme	harmful	1371:1377	arg1	bacteria					1379:1386	potentially harmful bacteria	1359:1386	potentially harmful bacteria (e.g., Bacteroids, and Mucispirillum)	1359:1424	Moreover, TEs are capable of exerting a prebiotic effect on gut microbiota by increasing the abundance of potentially beneficial bacteria (e.g., Faecalibaculum, and Bifidobacterium), and decreasing the abundance of potentially harmful bacteria (e.g., Bacteroids, and Mucispirillum).
31951100	4	52	dep	cytokines	651:659	arg1	cytokines					651:659	pro-inflammatory cytokines	634:659	pro-inflammatory cytokines (IL-6, IL-12, and tumor necrosis factor-α)	634:702	The tea extracts (TEs) significantly decrease the production of pro-inflammatory cytokines (IL-6, IL-12, and tumor necrosis factor-α) and increase the anti-inflammatory cytokines (IL-10) in LPS-stimulated RAW 264.7 macrophages and a dextran sodium sulfate (DSS)-induced colitis mouse model.
31951100	4	52	dep	cytokines	651:659	arg1	factor-α					694:701	tumor necrosis factor-α	679:701	tumor necrosis factor-α	679:701	The tea extracts (TEs) significantly decrease the production of pro-inflammatory cytokines (IL-6, IL-12, and tumor necrosis factor-α) and increase the anti-inflammatory cytokines (IL-10) in LPS-stimulated RAW 264.7 macrophages and a dextran sodium sulfate (DSS)-induced colitis mouse model.
31951100	4	52	dep	cytokines	651:659	arg1	IL-12					668:672	IL-12	668:672	IL-12	668:672	The tea extracts (TEs) significantly decrease the production of pro-inflammatory cytokines (IL-6, IL-12, and tumor necrosis factor-α) and increase the anti-inflammatory cytokines (IL-10) in LPS-stimulated RAW 264.7 macrophages and a dextran sodium sulfate (DSS)-induced colitis mouse model.
31951100	4	52	dep	cytokines	651:659	arg1	IL-6					662:665	IL-6	662:665	IL-6	662:665	The tea extracts (TEs) significantly decrease the production of pro-inflammatory cytokines (IL-6, IL-12, and tumor necrosis factor-α) and increase the anti-inflammatory cytokines (IL-10) in LPS-stimulated RAW 264.7 macrophages and a dextran sodium sulfate (DSS)-induced colitis mouse model.
31951100	4	53	theme	cytokines	651:659	arg1	production					620:629	the production	616:629	the production of pro-inflammatory cytokines (IL-6, IL-12, and tumor necrosis factor-α)	616:702	The tea extracts (TEs) significantly decrease the production of pro-inflammatory cytokines (IL-6, IL-12, and tumor necrosis factor-α) and increase the anti-inflammatory cytokines (IL-10) in LPS-stimulated RAW 264.7 macrophages and a dextran sodium sulfate (DSS)-induced colitis mouse model.
31951100	8	54	from	production	1453:1462	arg1	feces					1480:1484	the feces	1476:1484	the feces of colitic mice	1476:1500	TEs restore the decreased production of SCFAs in the feces of colitic mice.
31951100	2	55	theme	yellow	368:373	arg1	tea					400:402	white, yellow, oolong, black, and dark tea	361:402	white, yellow, oolong, black, and dark tea	361:402	The in vivo and in vitro anti-inflammatory effects of Camellia sinensis (green, white, yellow, oolong, black, and dark tea) and Litsea coreana (hawk tea) are comparatively explored.
31951100	0	56	theme	Intestinal	48:57	arg1	Inflammation					59:70	Intestinal Inflammation	48:70	Intestinal Inflammation	48:70	Camellia sinensis and Litsea coreana Ameliorate Intestinal Inflammation and Modulate Gut Microbiota in Dextran Sulfate Sodium-Induced Colitis Mice.
31951100	7	57	dep	bacteria	1379:1386	arg1	Bacteroids					1395:1404	Bacteroids	1395:1404	Bacteroids	1395:1404	Moreover, TEs are capable of exerting a prebiotic effect on gut microbiota by increasing the abundance of potentially beneficial bacteria (e.g., Faecalibaculum, and Bifidobacterium), and decreasing the abundance of potentially harmful bacteria (e.g., Bacteroids, and Mucispirillum).
31951100	7	57	dep	bacteria	1379:1386	arg1	Mucispirillum					1411:1423	Mucispirillum	1411:1423	Mucispirillum	1411:1423	Moreover, TEs are capable of exerting a prebiotic effect on gut microbiota by increasing the abundance of potentially beneficial bacteria (e.g., Faecalibaculum, and Bifidobacterium), and decreasing the abundance of potentially harmful bacteria (e.g., Bacteroids, and Mucispirillum).
31951100	7	57	dep	bacteria	1379:1386	arg1	e.g.					1389:1392	e.g.	1389:1392	e.g.	1389:1392	Moreover, TEs are capable of exerting a prebiotic effect on gut microbiota by increasing the abundance of potentially beneficial bacteria (e.g., Faecalibaculum, and Bifidobacterium), and decreasing the abundance of potentially harmful bacteria (e.g., Bacteroids, and Mucispirillum).
31951100	5	58	theme	factor-κB	1020:1028	arg1	activation					998:1007	the activation	994:1007	the activation of nuclear factor-κB	994:1028	The treatment of TEs in colitis mice can ameliorate colon inflammation, pro-oxidative enzyme activity, colon integrity, and suppress the activation of nuclear factor-κB.
31951100	5	59	theme	nuclear	1012:1018	arg1	factor-κB					1020:1028	nuclear factor-κB	1012:1028	nuclear factor-κB	1012:1028	The treatment of TEs in colitis mice can ameliorate colon inflammation, pro-oxidative enzyme activity, colon integrity, and suppress the activation of nuclear factor-κB.
31951100	3	60	theme	HPLC	483:486	arg1	analysis					488:495	METHODS AND RESULTS HPLC analysis	463:495	METHODS AND RESULTS HPLC analysis	463:495	METHODS AND RESULTS HPLC analysis confirms dissimilarities among phytochemical compositions of these teas.
31951100	7	61	from	effect	1194:1199	arg1	microbiota					1208:1217	gut microbiota	1204:1217	gut microbiota	1204:1217	Moreover, TEs are capable of exerting a prebiotic effect on gut microbiota by increasing the abundance of potentially beneficial bacteria (e.g., Faecalibaculum, and Bifidobacterium), and decreasing the abundance of potentially harmful bacteria (e.g., Bacteroids, and Mucispirillum).
31951100	7	62	dep	bacteria	1273:1280	arg1	e.g.					1283:1286	e.g.	1283:1286	e.g.	1283:1286	Moreover, TEs are capable of exerting a prebiotic effect on gut microbiota by increasing the abundance of potentially beneficial bacteria (e.g., Faecalibaculum, and Bifidobacterium), and decreasing the abundance of potentially harmful bacteria (e.g., Bacteroids, and Mucispirillum).
31951100	7	62	dep	bacteria	1273:1280	arg1	Faecalibaculum					1289:1302	Faecalibaculum	1289:1302	Faecalibaculum	1289:1302	Moreover, TEs are capable of exerting a prebiotic effect on gut microbiota by increasing the abundance of potentially beneficial bacteria (e.g., Faecalibaculum, and Bifidobacterium), and decreasing the abundance of potentially harmful bacteria (e.g., Bacteroids, and Mucispirillum).
31951100	7	62	dep	bacteria	1273:1280	arg1	Bifidobacterium					1309:1323	Bifidobacterium	1309:1323	Bifidobacterium	1309:1323	Moreover, TEs are capable of exerting a prebiotic effect on gut microbiota by increasing the abundance of potentially beneficial bacteria (e.g., Faecalibaculum, and Bifidobacterium), and decreasing the abundance of potentially harmful bacteria (e.g., Bacteroids, and Mucispirillum).
31951100	2	63	theme	white	361:365	arg1	tea					400:402	white, yellow, oolong, black, and dark tea	361:402	white, yellow, oolong, black, and dark tea	361:402	The in vivo and in vitro anti-inflammatory effects of Camellia sinensis (green, white, yellow, oolong, black, and dark tea) and Litsea coreana (hawk tea) are comparatively explored.
31951100	3	64	theme	teas	564:567	arg1	compositions					542:553	phytochemical compositions	528:553	phytochemical compositions of these teas	528:567	METHODS AND RESULTS HPLC analysis confirms dissimilarities among phytochemical compositions of these teas.
31951100	1	65	theme	induced	264:270	arg1	colitis					272:278	experimentally induced colitis	249:278	experimentally induced colitis	249:278	SCOPE Polyphenol-enriched herbal extracts have been proved as alternative therapeutic strategies for experimentally induced colitis.
31951100	4	66	theme	RAW	775:777	arg1	macrophages					785:795	LPS-stimulated RAW 264.7 macrophages	760:795	LPS-stimulated RAW 264.7 macrophages	760:795	The tea extracts (TEs) significantly decrease the production of pro-inflammatory cytokines (IL-6, IL-12, and tumor necrosis factor-α) and increase the anti-inflammatory cytokines (IL-10) in LPS-stimulated RAW 264.7 macrophages and a dextran sodium sulfate (DSS)-induced colitis mouse model.
31951100	5	67	theme	colitis	885:891	arg1	mice					893:896	colitis mice	885:896	colitis mice	885:896	The treatment of TEs in colitis mice can ameliorate colon inflammation, pro-oxidative enzyme activity, colon integrity, and suppress the activation of nuclear factor-κB.
31951100	1	68	theme	SCOPE	148:152	arg1	extracts					181:188	SCOPE Polyphenol-enriched herbal extracts	148:188	SCOPE Polyphenol-enriched herbal extracts	148:188	SCOPE Polyphenol-enriched herbal extracts have been proved as alternative therapeutic strategies for experimentally induced colitis.
31951100	3	69	theme	METHODS	463:469	arg1	analysis					488:495	METHODS AND RESULTS HPLC analysis	463:495	METHODS AND RESULTS HPLC analysis	463:495	METHODS AND RESULTS HPLC analysis confirms dissimilarities among phytochemical compositions of these teas.
31951100	6	70	theme	DSS-induced	1078:1088	arg1	decrease					1090:1097	the DSS-induced decrease	1074:1097	the DSS-induced decrease in richness and diversity of gut microbiota	1074:1141	Of note, green TE significantly attenuates the DSS-induced decrease in richness and diversity of gut microbiota.
31951100	1	71	theme	therapeutic	222:232	arg1	strategies					234:243	alternative therapeutic strategies	210:243	alternative therapeutic strategies for experimentally induced colitis	210:278	SCOPE Polyphenol-enriched herbal extracts have been proved as alternative therapeutic strategies for experimentally induced colitis.
31951100	2	72	theme	black	384:388	arg1	tea					400:402	white, yellow, oolong, black, and dark tea	361:402	white, yellow, oolong, black, and dark tea	361:402	The in vivo and in vitro anti-inflammatory effects of Camellia sinensis (green, white, yellow, oolong, black, and dark tea) and Litsea coreana (hawk tea) are comparatively explored.
31951100	7	73	theme	gut	1204:1206	arg1	microbiota					1208:1217	gut microbiota	1204:1217	gut microbiota	1204:1217	Moreover, TEs are capable of exerting a prebiotic effect on gut microbiota by increasing the abundance of potentially beneficial bacteria (e.g., Faecalibaculum, and Bifidobacterium), and decreasing the abundance of potentially harmful bacteria (e.g., Bacteroids, and Mucispirillum).
31951100	8	74	theme	SCFAs	1467:1471	arg1	production					1453:1462	the decreased production	1439:1462	the decreased production of SCFAs in the feces of colitic mice	1439:1500	TEs restore the decreased production of SCFAs in the feces of colitic mice.
31951100	4	75	theme	pro-inflammatory	634:649	arg1	cytokines					651:659	pro-inflammatory cytokines	634:659	pro-inflammatory cytokines (IL-6, IL-12, and tumor necrosis factor-α)	634:702	The tea extracts (TEs) significantly decrease the production of pro-inflammatory cytokines (IL-6, IL-12, and tumor necrosis factor-α) and increase the anti-inflammatory cytokines (IL-10) in LPS-stimulated RAW 264.7 macrophages and a dextran sodium sulfate (DSS)-induced colitis mouse model.
31951100	4	75	theme	pro-inflammatory	634:649	arg1	factor-α					694:701	tumor necrosis factor-α	679:701	tumor necrosis factor-α	679:701	The tea extracts (TEs) significantly decrease the production of pro-inflammatory cytokines (IL-6, IL-12, and tumor necrosis factor-α) and increase the anti-inflammatory cytokines (IL-10) in LPS-stimulated RAW 264.7 macrophages and a dextran sodium sulfate (DSS)-induced colitis mouse model.
31951100	4	75	theme	pro-inflammatory	634:649	arg1	IL-12					668:672	IL-12	668:672	IL-12	668:672	The tea extracts (TEs) significantly decrease the production of pro-inflammatory cytokines (IL-6, IL-12, and tumor necrosis factor-α) and increase the anti-inflammatory cytokines (IL-10) in LPS-stimulated RAW 264.7 macrophages and a dextran sodium sulfate (DSS)-induced colitis mouse model.
31951100	4	75	theme	pro-inflammatory	634:649	arg1	IL-6					662:665	IL-6	662:665	IL-6	662:665	The tea extracts (TEs) significantly decrease the production of pro-inflammatory cytokines (IL-6, IL-12, and tumor necrosis factor-α) and increase the anti-inflammatory cytokines (IL-10) in LPS-stimulated RAW 264.7 macrophages and a dextran sodium sulfate (DSS)-induced colitis mouse model.
31951100	6	76	from	decrease	1090:1097	arg1	richness					1102:1109	richness	1102:1109	richness	1102:1109	Of note, green TE significantly attenuates the DSS-induced decrease in richness and diversity of gut microbiota.
31951100	6	76	from	decrease	1090:1097	arg1	diversity					1115:1123	diversity	1115:1123	diversity	1115:1123	Of note, green TE significantly attenuates the DSS-induced decrease in richness and diversity of gut microbiota.
31951100	1	77	theme	herbal	174:179	arg1	extracts					181:188	SCOPE Polyphenol-enriched herbal extracts	148:188	SCOPE Polyphenol-enriched herbal extracts	148:188	SCOPE Polyphenol-enriched herbal extracts have been proved as alternative therapeutic strategies for experimentally induced colitis.
31951100	9	78	from	dysbiosis	1610:1618	arg1	mice					1649:1652	colitis mice	1641:1652	colitis mice	1641:1652	CONCLUSION The treatment of seven types of tea can alleviate DSS-induced colitis in mice, and modulate the dysbiosis of gut microbiota in colitis mice.
32979713	0	0	theme	enzyme	71:76	arg1	activities					78:87	pectinolytic enzyme activities	58:87	pectinolytic enzyme activities	58:87	Changes in cell wall neutral sugar composition related to pectinolytic enzyme activities and intra-flesh textural property during ripening of ten apricot clones.
32979713	6	1	theme	enzymatic	884:892	arg1	activity					894:901	enzymatic activity	884:901	enzymatic activity	884:901	The ripening effect has been observed in firmness evolution according to enzymatic activity.
32979713	7	2	theme	clones	949:954	arg1	classification					931:944	a classification	929:944	a classification of clones	929:954	This correlation allowed a classification of clones depending on softening.
32979713	0	3	theme	intra-flesh	93:103	arg1	property					114:121	intra-flesh textural property	93:121	intra-flesh textural property	93:121	Changes in cell wall neutral sugar composition related to pectinolytic enzyme activities and intra-flesh textural property during ripening of ten apricot clones.
32979713	0	4	from	Changes	0:6	arg1	composition					35:45	cell wall neutral sugar composition	11:45	cell wall neutral sugar composition	11:45	Changes in cell wall neutral sugar composition related to pectinolytic enzyme activities and intra-flesh textural property during ripening of ten apricot clones.
32979713	2	5	theme	Fruit	282:286	arg1	firmness					288:295	Fruit firmness	282:295	Fruit firmness	282:295	Fruit firmness, cell wall composition and enzyme activity of three apricot flesh zones were analysed.
32979713	4	6	theme	methylesterification	566:585	arg1	degree					587:592	The methylesterification degree	562:592	The methylesterification degree	562:592	The methylesterification degree varied significantly among the different clones ranging from 58 to 97 in Ab 5 and Mans 15 respectively.
32979713	3	7	theme	118-214 mg g-1	541:554	arg1	glucose					532:538	cellulosic glucose	521:538	cellulosic glucose (118-214 mg g-1 AIS)	521:559	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	3	7	theme	118-214 mg g-1	541:554	arg1	AIS					556:558	118-214 mg g-1 AIS	541:558	118-214 mg g-1 AIS	541:558	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	3	8	theme	cellulosic	521:530	arg1	glucose					532:538	cellulosic glucose	521:538	cellulosic glucose (118-214 mg g-1 AIS)	521:559	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	3	8	theme	cellulosic	521:530	arg1	AIS					556:558	118-214 mg g-1 AIS	541:558	118-214 mg g-1 AIS	541:558	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	5	9	theme	zones	712:716	arg1	firmness					718:725	zones firmness	712:725	zones firmness	712:725	Conversely to zones firmness, enzymatic activity was higher in pistil followed by equatorial and peduncle zones.
32979713	3	10	theme	high	505:508	arg1	AIS					485:487	179-300 mg g-1 AIS	470:487	179-300 mg g-1 AIS	470:487	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	3	10	theme	high	505:508	arg1	AIS					556:558	118-214 mg g-1 AIS	541:558	118-214 mg g-1 AIS	541:558	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	3	10	theme	high	505:508	arg1	amounts					510:516	relatively high amounts	494:516	relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS)	494:559	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	3	10	theme	high	505:508	arg1	glucose					532:538	cellulosic glucose	521:538	cellulosic glucose (118-214 mg g-1 AIS)	521:559	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	3	10	theme	high	505:508	arg1	acid					464:467	uronic acid	457:467	uronic acid (179-300 mg g-1 AIS)	457:488	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	0	11	theme	textural	105:112	arg1	property					114:121	intra-flesh textural property	93:121	intra-flesh textural property	93:121	Changes in cell wall neutral sugar composition related to pectinolytic enzyme activities and intra-flesh textural property during ripening of ten apricot clones.
32979713	6	12	theme	firmness	852:859	arg1	evolution					861:869	firmness evolution	852:869	firmness evolution	852:869	The ripening effect has been observed in firmness evolution according to enzymatic activity.
32979713	8	13	theme	processing	1134:1143	arg1	industry					1145:1152	the processing industry	1130:1152	the processing industry	1130:1152	Among studied clones, Ab 5, Marouch 16, Mans 15 and Cg 2 were less influenced by softening and have the advantage of a technological valorisation for the processing industry.
32979713	8	14	theme	studied	986:992	arg1	clones					994:999	studied clones	986:999	studied clones	986:999	Among studied clones, Ab 5, Marouch 16, Mans 15 and Cg 2 were less influenced by softening and have the advantage of a technological valorisation for the processing industry.
32979713	2	15	theme	wall	303:306	arg1	composition					308:318	cell wall composition	298:318	cell wall composition	298:318	Fruit firmness, cell wall composition and enzyme activity of three apricot flesh zones were analysed.
32979713	1	16	theme	texture	177:183	arg1	changes					166:172	The changes	162:172	The changes of texture	162:183	The changes of texture and cell wall characteristics of apricot were investigated in ten clones at two maturity stages.
32979713	1	16	theme	texture	177:183	arg1	characteristics					199:213	cell wall characteristics	189:213	cell wall characteristics of apricot	189:224	The changes of texture and cell wall characteristics of apricot were investigated in ten clones at two maturity stages.
32979713	5	17	theme	equatorial	780:789	arg1	zones					804:808	equatorial and peduncle zones	780:808	equatorial and peduncle zones	780:808	Conversely to zones firmness, enzymatic activity was higher in pistil followed by equatorial and peduncle zones.
32979713	1	18	theme	maturity	265:272	arg1	stages					274:279	two maturity stages	261:279	two maturity stages	261:279	The changes of texture and cell wall characteristics of apricot were investigated in ten clones at two maturity stages.
32979713	2	19	theme	cell	298:301	arg1	composition					308:318	cell wall composition	298:318	cell wall composition	298:318	Fruit firmness, cell wall composition and enzyme activity of three apricot flesh zones were analysed.
32979713	2	20	theme	zones	363:367	arg1	composition					308:318	cell wall composition	298:318	cell wall composition	298:318	Fruit firmness, cell wall composition and enzyme activity of three apricot flesh zones were analysed.
32979713	2	20	theme	zones	363:367	arg1	activity					331:338	enzyme activity	324:338	enzyme activity	324:338	Fruit firmness, cell wall composition and enzyme activity of three apricot flesh zones were analysed.
32979713	2	20	theme	zones	363:367	arg1	firmness					288:295	Fruit firmness	282:295	Fruit firmness	282:295	Fruit firmness, cell wall composition and enzyme activity of three apricot flesh zones were analysed.
32979713	0	21	theme	wall	16:19	arg1	composition					35:45	cell wall neutral sugar composition	11:45	cell wall neutral sugar composition	11:45	Changes in cell wall neutral sugar composition related to pectinolytic enzyme activities and intra-flesh textural property during ripening of ten apricot clones.
32979713	8	22	theme	valorisation	1113:1124	arg1	advantage					1084:1092	the advantage	1080:1092	the advantage of a technological valorisation for the processing industry	1080:1152	Among studied clones, Ab 5, Marouch 16, Mans 15 and Cg 2 were less influenced by softening and have the advantage of a technological valorisation for the processing industry.
32979713	3	23	theme	glucose	532:538	arg1	glucose					532:538	cellulosic glucose	521:538	cellulosic glucose (118-214 mg g-1 AIS)	521:559	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	3	23	theme	glucose	532:538	arg1	acid					464:467	uronic acid	457:467	uronic acid (179-300 mg g-1 AIS)	457:488	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	3	23	theme	glucose	532:538	arg1	AIS					556:558	118-214 mg g-1 AIS	541:558	118-214 mg g-1 AIS	541:558	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	3	23	theme	glucose	532:538	arg1	amounts					510:516	relatively high amounts	494:516	relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS)	494:559	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	3	23	theme	glucose	532:538	arg1	amounts					446:452	high amounts	441:452	high amounts of uronic acid (179-300 mg g-1 AIS)	441:488	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	3	23	theme	glucose	532:538	arg1	AIS					485:487	179-300 mg g-1 AIS	470:487	179-300 mg g-1 AIS	470:487	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	1	24	theme	cell	189:192	arg1	characteristics					199:213	cell wall characteristics	189:213	cell wall characteristics of apricot	189:224	The changes of texture and cell wall characteristics of apricot were investigated in ten clones at two maturity stages.
32979713	0	25	theme	cell	11:14	arg1	composition					35:45	cell wall neutral sugar composition	11:45	cell wall neutral sugar composition	11:45	Changes in cell wall neutral sugar composition related to pectinolytic enzyme activities and intra-flesh textural property during ripening of ten apricot clones.
32979713	6	26	theme	ripening	815:822	arg1	effect					824:829	The ripening effect	811:829	The ripening effect	811:829	The ripening effect has been observed in firmness evolution according to enzymatic activity.
32979713	1	27	theme	wall	194:197	arg1	characteristics					199:213	cell wall characteristics	189:213	cell wall characteristics of apricot	189:224	The changes of texture and cell wall characteristics of apricot were investigated in ten clones at two maturity stages.
32979713	0	28	theme	sugar	29:33	arg1	composition					35:45	cell wall neutral sugar composition	11:45	cell wall neutral sugar composition	11:45	Changes in cell wall neutral sugar composition related to pectinolytic enzyme activities and intra-flesh textural property during ripening of ten apricot clones.
32979713	3	29	theme	179-300 mg g-1	470:483	arg1	acid					464:467	uronic acid	457:467	uronic acid (179-300 mg g-1 AIS)	457:488	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	3	29	theme	179-300 mg g-1	470:483	arg1	AIS					485:487	179-300 mg g-1 AIS	470:487	179-300 mg g-1 AIS	470:487	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	0	30	theme	neutral	21:27	arg1	composition					35:45	cell wall neutral sugar composition	11:45	cell wall neutral sugar composition	11:45	Changes in cell wall neutral sugar composition related to pectinolytic enzyme activities and intra-flesh textural property during ripening of ten apricot clones.
32979713	3	31	theme	alcohol-insoluble	393:409	arg1	AIS					388:390	The AIS	384:390	The AIS (alcohol-insoluble solids)	384:417	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	3	31	theme	alcohol-insoluble	393:409	arg1	solids					411:416	alcohol-insoluble solids	393:416	alcohol-insoluble solids	393:416	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	3	32	theme	high	441:444	arg1	AIS					485:487	179-300 mg g-1 AIS	470:487	179-300 mg g-1 AIS	470:487	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	3	32	theme	high	441:444	arg1	amounts					446:452	high amounts	441:452	high amounts of uronic acid (179-300 mg g-1 AIS)	441:488	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	3	32	theme	high	441:444	arg1	acid					464:467	uronic acid	457:467	uronic acid (179-300 mg g-1 AIS)	457:488	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	3	32	theme	high	441:444	arg1	glucose					532:538	cellulosic glucose	521:538	cellulosic glucose (118-214 mg g-1 AIS)	521:559	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	3	32	theme	high	441:444	arg1	AIS					556:558	118-214 mg g-1 AIS	541:558	118-214 mg g-1 AIS	541:558	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	0	33	theme	related	47:53	arg1	Changes					0:6	Changes	0:6	Changes in cell wall neutral sugar composition related to pectinolytic enzyme activities	0:87	Changes in cell wall neutral sugar composition related to pectinolytic enzyme activities and intra-flesh textural property during ripening of ten apricot clones.
32979713	8	34	theme	technological	1099:1111	arg1	valorisation					1113:1124	a technological valorisation	1097:1124	a technological valorisation	1097:1124	Among studied clones, Ab 5, Marouch 16, Mans 15 and Cg 2 were less influenced by softening and have the advantage of a technological valorisation for the processing industry.
32979713	0	35	theme	clones	154:159	arg1	ripening					130:137	ripening	130:137	ripening of ten apricot clones	130:159	Changes in cell wall neutral sugar composition related to pectinolytic enzyme activities and intra-flesh textural property during ripening of ten apricot clones.
32979713	6	36	located	observed	840:847	arg1	evolution					861:869	firmness evolution	852:869	firmness evolution	852:869	The ripening effect has been observed in firmness evolution according to enzymatic activity.
32979713	6	36	located	observed	840:847	arg2	effect					824:829	The ripening effect	811:829	The ripening effect	811:829	The ripening effect has been observed in firmness evolution according to enzymatic activity.
32979713	0	37	theme	apricot	146:152	arg1	clones					154:159	ten apricot clones	142:159	ten apricot clones	142:159	Changes in cell wall neutral sugar composition related to pectinolytic enzyme activities and intra-flesh textural property during ripening of ten apricot clones.
32979713	0	38	from	property	114:121	arg1	composition					35:45	cell wall neutral sugar composition	11:45	cell wall neutral sugar composition	11:45	Changes in cell wall neutral sugar composition related to pectinolytic enzyme activities and intra-flesh textural property during ripening of ten apricot clones.
32979713	2	39	theme	enzyme	324:329	arg1	activity					331:338	enzyme activity	324:338	enzyme activity	324:338	Fruit firmness, cell wall composition and enzyme activity of three apricot flesh zones were analysed.
32979713	4	40	dep	97	661:662	arg1	to					658:659	to	658:659	to	658:659	The methylesterification degree varied significantly among the different clones ranging from 58 to 97 in Ab 5 and Mans 15 respectively.
32979713	3	41	theme	uronic	457:462	arg1	acid					464:467	uronic acid	457:467	uronic acid (179-300 mg g-1 AIS)	457:488	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	3	41	theme	uronic	457:462	arg1	AIS					485:487	179-300 mg g-1 AIS	470:487	179-300 mg g-1 AIS	470:487	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	8	42	contain	have	1075:1078	arg2	advantage					1084:1092	the advantage	1080:1092	the advantage of a technological valorisation for the processing industry	1080:1152	Among studied clones, Ab 5, Marouch 16, Mans 15 and Cg 2 were less influenced by softening and have the advantage of a technological valorisation for the processing industry.
32979713	8	42	contain	have	1075:1078	arg1	Ab					1002:1003	Ab 5	1002:1005	Ab 5	1002:1005	Among studied clones, Ab 5, Marouch 16, Mans 15 and Cg 2 were less influenced by softening and have the advantage of a technological valorisation for the processing industry.
32979713	1	43	theme	apricot	218:224	arg1	changes					166:172	The changes	162:172	The changes of texture	162:183	The changes of texture and cell wall characteristics of apricot were investigated in ten clones at two maturity stages.
32979713	1	43	theme	apricot	218:224	arg1	characteristics					199:213	cell wall characteristics	189:213	cell wall characteristics of apricot	189:224	The changes of texture and cell wall characteristics of apricot were investigated in ten clones at two maturity stages.
32979713	3	44	theme	acid	464:467	arg1	glucose					532:538	cellulosic glucose	521:538	cellulosic glucose (118-214 mg g-1 AIS)	521:559	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	3	44	theme	acid	464:467	arg1	acid					464:467	uronic acid	457:467	uronic acid (179-300 mg g-1 AIS)	457:488	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	3	44	theme	acid	464:467	arg1	AIS					556:558	118-214 mg g-1 AIS	541:558	118-214 mg g-1 AIS	541:558	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	3	44	theme	acid	464:467	arg1	amounts					510:516	relatively high amounts	494:516	relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS)	494:559	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	3	44	theme	acid	464:467	arg1	amounts					446:452	high amounts	441:452	high amounts of uronic acid (179-300 mg g-1 AIS)	441:488	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	3	44	theme	acid	464:467	arg1	AIS					485:487	179-300 mg g-1 AIS	470:487	179-300 mg g-1 AIS	470:487	The AIS (alcohol-insoluble solids) were characterised by high amounts of uronic acid (179-300 mg g-1 AIS) and relatively high amounts of cellulosic glucose (118-214 mg g-1 AIS).
32979713	0	45	theme	pectinolytic	58:69	arg1	activities					78:87	pectinolytic enzyme activities	58:87	pectinolytic enzyme activities	58:87	Changes in cell wall neutral sugar composition related to pectinolytic enzyme activities and intra-flesh textural property during ripening of ten apricot clones.
32979713	4	46	theme	different	625:633	arg1	clones					635:640	the different clones	621:640	the different clones ranging from 58 to 97 in Ab 5 and Mans 15 respectively	621:695	The methylesterification degree varied significantly among the different clones ranging from 58 to 97 in Ab 5 and Mans 15 respectively.
32979713	2	47	theme	flesh	357:361	arg1	zones					363:367	three apricot flesh zones	343:367	three apricot flesh zones	343:367	Fruit firmness, cell wall composition and enzyme activity of three apricot flesh zones were analysed.
32979713	5	48	theme	enzymatic	728:736	arg1	activity					738:745	enzymatic activity	728:745	enzymatic activity	728:745	Conversely to zones firmness, enzymatic activity was higher in pistil followed by equatorial and peduncle zones.
32979713	5	49	theme	peduncle	795:802	arg1	zones					804:808	equatorial and peduncle zones	780:808	equatorial and peduncle zones	780:808	Conversely to zones firmness, enzymatic activity was higher in pistil followed by equatorial and peduncle zones.
32979713	2	50	theme	apricot	349:355	arg1	zones					363:367	three apricot flesh zones	343:367	three apricot flesh zones	343:367	Fruit firmness, cell wall composition and enzyme activity of three apricot flesh zones were analysed.
34052269	4	0	dep	in	886:887	arg1	vitro					889:893	vitro	889:893	vitro	889:893	Furthermore, in vitro evaluation of chondrogenic potential showed that the MAF provided better cell adhesion, viability, proliferation and GAG secretion than the MBF.
34052269	5	1	theme	tissue	1096:1101	arg1	engineering					1103:1113	auricular cartilage tissue engineering	1076:1113	auricular cartilage tissue engineering	1076:1113	Therefore, the MAF are promising in auricular cartilage tissue engineering and relevant plastic surgery-related applications.
34052269	1	2	theme	composite	163:171	arg1	scaffolds					173:181	clinical applicable polymeric composite scaffolds	133:181	clinical applicable polymeric composite scaffolds	133:181	Designing clinical applicable polymeric composite scaffolds for auricular cartilage tissue engineering requires appropriate mechanical strength and biological characteristics.
34052269	4	3	theme	in	886:887	arg1	evaluation					895:904	in vitro evaluation	886:904	in vitro evaluation of chondrogenic potential	886:930	Furthermore, in vitro evaluation of chondrogenic potential showed that the MAF provided better cell adhesion, viability, proliferation and GAG secretion than the MBF.
34052269	0	4	theme	auricular	83:91	arg1	cartilage					93:101	auricular cartilage	83:101	auricular cartilage	83:101	Non-mulberry silk fiber-based scaffolds reinforced by PLLA porous microspheres for auricular cartilage: An in vitro study.
34052269	3	5	theme	PMs	703:705	arg1	MAF					708:710	PLLA PMs (MAF)	698:711	PLLA PMs (MAF)	698:711	We found that the Ap silk fiber-based scaffolds reinforced by PLLA PMs (MAF) exhibited superior physical properties (the mechanical properties in particular) as compared to the Bm silk fiber-based scaffolds reinforced by PLLA PMs (MBF).
34052269	0	6	dep	study	116:120	arg1	scaffolds					30:38	Non-mulberry silk fiber-based scaffolds	0:38	Non-mulberry silk fiber-based scaffolds reinforced by PLLA porous microspheres for auricular cartilage	0:101	Non-mulberry silk fiber-based scaffolds reinforced by PLLA porous microspheres for auricular cartilage: An in vitro study.
34052269	5	7	theme	plastic	1128:1134	arg1	applications					1152:1163	relevant plastic surgery-related applications	1119:1163	relevant plastic surgery-related applications	1119:1163	Therefore, the MAF are promising in auricular cartilage tissue engineering and relevant plastic surgery-related applications.
34052269	3	8	theme	silk	816:819	arg1	scaffolds					833:841	the Bm silk fiber-based scaffolds	809:841	the Bm silk fiber-based scaffolds reinforced by PLLA PMs (MBF)	809:870	We found that the Ap silk fiber-based scaffolds reinforced by PLLA PMs (MAF) exhibited superior physical properties (the mechanical properties in particular) as compared to the Bm silk fiber-based scaffolds reinforced by PLLA PMs (MBF).
34052269	5	9	from	promising	1063:1071	arg1	applications					1152:1163	relevant plastic surgery-related applications	1119:1163	relevant plastic surgery-related applications	1119:1163	Therefore, the MAF are promising in auricular cartilage tissue engineering and relevant plastic surgery-related applications.
34052269	5	9	from	promising	1063:1071	arg1	engineering					1103:1113	auricular cartilage tissue engineering	1076:1113	auricular cartilage tissue engineering	1076:1113	Therefore, the MAF are promising in auricular cartilage tissue engineering and relevant plastic surgery-related applications.
34052269	3	10	theme	physical	732:739	arg1	properties					741:750	superior physical properties	723:750	superior physical properties (the mechanical properties in particular)	723:792	We found that the Ap silk fiber-based scaffolds reinforced by PLLA PMs (MAF) exhibited superior physical properties (the mechanical properties in particular) as compared to the Bm silk fiber-based scaffolds reinforced by PLLA PMs (MBF).
34052269	2	11	theme	fiber-based	319:329	arg1	scaffolds					331:339	silk fiber-based scaffolds	314:339	silk fiber-based scaffolds co-reinforced with poly-L-lactic acid porous microspheres (PLLA PMs) combined with either Bombyx mori (Bm) or Antheraea pernyi (Ap) silk fibers	314:483	In this study, silk fiber-based scaffolds co-reinforced with poly-L-lactic acid porous microspheres (PLLA PMs) combined with either Bombyx mori (Bm) or Antheraea pernyi (Ap) silk fibers were fabricated as inspired by the "steel bars reinforced concrete" structure in architecture and their chondrogenic functions were also investigated.
34052269	5	12	from	applications	1152:1163	arg1	MAF					1055:1057	the MAF	1051:1057	the MAF	1051:1057	Therefore, the MAF are promising in auricular cartilage tissue engineering and relevant plastic surgery-related applications.
34052269	5	12	from	applications	1152:1163	arg1	promising					1063:1071	promising	1063:1071	promising	1063:1071	Therefore, the MAF are promising in auricular cartilage tissue engineering and relevant plastic surgery-related applications.
34052269	0	13	dep	in	107:108	arg1	vitro					110:114	vitro	110:114	vitro	110:114	Non-mulberry silk fiber-based scaffolds reinforced by PLLA porous microspheres for auricular cartilage: An in vitro study.
34052269	2	14	theme	porous	379:384	arg1	microspheres					386:397	poly-L-lactic acid porous microspheres	360:397	poly-L-lactic acid porous microspheres (PLLA PMs) combined with either Bombyx mori (Bm) or Antheraea pernyi (Ap) silk fibers	360:483	In this study, silk fiber-based scaffolds co-reinforced with poly-L-lactic acid porous microspheres (PLLA PMs) combined with either Bombyx mori (Bm) or Antheraea pernyi (Ap) silk fibers were fabricated as inspired by the "steel bars reinforced concrete" structure in architecture and their chondrogenic functions were also investigated.
34052269	2	14	theme	porous	379:384	arg1	PMs					405:407	PLLA PMs	400:407	PLLA PMs	400:407	In this study, silk fiber-based scaffolds co-reinforced with poly-L-lactic acid porous microspheres (PLLA PMs) combined with either Bombyx mori (Bm) or Antheraea pernyi (Ap) silk fibers were fabricated as inspired by the "steel bars reinforced concrete" structure in architecture and their chondrogenic functions were also investigated.
34052269	2	15	theme	steel	521:525	arg1	bars					527:530	the "steel bars	516:530	the "steel bars	516:530	In this study, silk fiber-based scaffolds co-reinforced with poly-L-lactic acid porous microspheres (PLLA PMs) combined with either Bombyx mori (Bm) or Antheraea pernyi (Ap) silk fibers were fabricated as inspired by the "steel bars reinforced concrete" structure in architecture and their chondrogenic functions were also investigated.
34052269	5	16	theme	auricular	1076:1084	arg1	engineering					1103:1113	auricular cartilage tissue engineering	1076:1113	auricular cartilage tissue engineering	1076:1113	Therefore, the MAF are promising in auricular cartilage tissue engineering and relevant plastic surgery-related applications.
34052269	2	17	theme	silk	314:317	arg1	scaffolds					331:339	silk fiber-based scaffolds	314:339	silk fiber-based scaffolds co-reinforced with poly-L-lactic acid porous microspheres (PLLA PMs) combined with either Bombyx mori (Bm) or Antheraea pernyi (Ap) silk fibers	314:483	In this study, silk fiber-based scaffolds co-reinforced with poly-L-lactic acid porous microspheres (PLLA PMs) combined with either Bombyx mori (Bm) or Antheraea pernyi (Ap) silk fibers were fabricated as inspired by the "steel bars reinforced concrete" structure in architecture and their chondrogenic functions were also investigated.
34052269	2	18	theme	Bombyx	431:436	arg1	mori					438:441	Bombyx mori	431:441	Bombyx mori (Bm)	431:446	In this study, silk fiber-based scaffolds co-reinforced with poly-L-lactic acid porous microspheres (PLLA PMs) combined with either Bombyx mori (Bm) or Antheraea pernyi (Ap) silk fibers were fabricated as inspired by the "steel bars reinforced concrete" structure in architecture and their chondrogenic functions were also investigated.
34052269	2	18	theme	Bombyx	431:436	arg1	Bm					444:445	Bm	444:445	Bm	444:445	In this study, silk fiber-based scaffolds co-reinforced with poly-L-lactic acid porous microspheres (PLLA PMs) combined with either Bombyx mori (Bm) or Antheraea pernyi (Ap) silk fibers were fabricated as inspired by the "steel bars reinforced concrete" structure in architecture and their chondrogenic functions were also investigated.
34052269	0	19	theme	in	107:108	arg1	study					116:120	An in vitro study	104:120	An in vitro study	104:120	Non-mulberry silk fiber-based scaffolds reinforced by PLLA porous microspheres for auricular cartilage: An in vitro study.
34052269	5	20	theme	cartilage	1086:1094	arg1	engineering					1103:1113	auricular cartilage tissue engineering	1076:1113	auricular cartilage tissue engineering	1076:1113	Therefore, the MAF are promising in auricular cartilage tissue engineering and relevant plastic surgery-related applications.
34052269	4	21	theme	GAG	1012:1014	arg1	secretion					1016:1024	GAG secretion	1012:1024	GAG secretion	1012:1024	Furthermore, in vitro evaluation of chondrogenic potential showed that the MAF provided better cell adhesion, viability, proliferation and GAG secretion than the MBF.
34052269	1	22	theme	auricular	187:195	arg1	engineering					214:224	auricular cartilage tissue engineering	187:224	auricular cartilage tissue engineering	187:224	Designing clinical applicable polymeric composite scaffolds for auricular cartilage tissue engineering requires appropriate mechanical strength and biological characteristics.
34052269	0	23	theme	silk	13:16	arg1	scaffolds					30:38	Non-mulberry silk fiber-based scaffolds	0:38	Non-mulberry silk fiber-based scaffolds reinforced by PLLA porous microspheres for auricular cartilage	0:101	Non-mulberry silk fiber-based scaffolds reinforced by PLLA porous microspheres for auricular cartilage: An in vitro study.
34052269	3	24	theme	superior	723:730	arg1	properties					741:750	superior physical properties	723:750	superior physical properties (the mechanical properties in particular)	723:792	We found that the Ap silk fiber-based scaffolds reinforced by PLLA PMs (MAF) exhibited superior physical properties (the mechanical properties in particular) as compared to the Bm silk fiber-based scaffolds reinforced by PLLA PMs (MBF).
34052269	2	25	theme	acid	374:377	arg1	microspheres					386:397	poly-L-lactic acid porous microspheres	360:397	poly-L-lactic acid porous microspheres (PLLA PMs) combined with either Bombyx mori (Bm) or Antheraea pernyi (Ap) silk fibers	360:483	In this study, silk fiber-based scaffolds co-reinforced with poly-L-lactic acid porous microspheres (PLLA PMs) combined with either Bombyx mori (Bm) or Antheraea pernyi (Ap) silk fibers were fabricated as inspired by the "steel bars reinforced concrete" structure in architecture and their chondrogenic functions were also investigated.
34052269	2	25	theme	acid	374:377	arg1	PMs					405:407	PLLA PMs	400:407	PLLA PMs	400:407	In this study, silk fiber-based scaffolds co-reinforced with poly-L-lactic acid porous microspheres (PLLA PMs) combined with either Bombyx mori (Bm) or Antheraea pernyi (Ap) silk fibers were fabricated as inspired by the "steel bars reinforced concrete" structure in architecture and their chondrogenic functions were also investigated.
34052269	1	26	theme	cartilage	197:205	arg1	engineering					214:224	auricular cartilage tissue engineering	187:224	auricular cartilage tissue engineering	187:224	Designing clinical applicable polymeric composite scaffolds for auricular cartilage tissue engineering requires appropriate mechanical strength and biological characteristics.
34052269	0	27	theme	Non-mulberry	0:11	arg1	scaffolds					30:38	Non-mulberry silk fiber-based scaffolds	0:38	Non-mulberry silk fiber-based scaffolds reinforced by PLLA porous microspheres for auricular cartilage	0:101	Non-mulberry silk fiber-based scaffolds reinforced by PLLA porous microspheres for auricular cartilage: An in vitro study.
34052269	3	28	theme	mechanical	757:766	arg1	properties					768:777	the mechanical properties	753:777	the mechanical properties in particular	753:791	We found that the Ap silk fiber-based scaffolds reinforced by PLLA PMs (MAF) exhibited superior physical properties (the mechanical properties in particular) as compared to the Bm silk fiber-based scaffolds reinforced by PLLA PMs (MBF).
34052269	3	29	from	properties	768:777	arg1	particular					782:791	particular	782:791	particular	782:791	We found that the Ap silk fiber-based scaffolds reinforced by PLLA PMs (MAF) exhibited superior physical properties (the mechanical properties in particular) as compared to the Bm silk fiber-based scaffolds reinforced by PLLA PMs (MBF).
34052269	2	30	theme	poly-L-lactic	360:372	arg1	microspheres					386:397	poly-L-lactic acid porous microspheres	360:397	poly-L-lactic acid porous microspheres (PLLA PMs) combined with either Bombyx mori (Bm) or Antheraea pernyi (Ap) silk fibers	360:483	In this study, silk fiber-based scaffolds co-reinforced with poly-L-lactic acid porous microspheres (PLLA PMs) combined with either Bombyx mori (Bm) or Antheraea pernyi (Ap) silk fibers were fabricated as inspired by the "steel bars reinforced concrete" structure in architecture and their chondrogenic functions were also investigated.
34052269	2	30	theme	poly-L-lactic	360:372	arg1	PMs					405:407	PLLA PMs	400:407	PLLA PMs	400:407	In this study, silk fiber-based scaffolds co-reinforced with poly-L-lactic acid porous microspheres (PLLA PMs) combined with either Bombyx mori (Bm) or Antheraea pernyi (Ap) silk fibers were fabricated as inspired by the "steel bars reinforced concrete" structure in architecture and their chondrogenic functions were also investigated.
34052269	5	31	theme	relevant	1119:1126	arg1	applications					1152:1163	relevant plastic surgery-related applications	1119:1163	relevant plastic surgery-related applications	1119:1163	Therefore, the MAF are promising in auricular cartilage tissue engineering and relevant plastic surgery-related applications.
34052269	1	32	theme	tissue	207:212	arg1	engineering					214:224	auricular cartilage tissue engineering	187:224	auricular cartilage tissue engineering	187:224	Designing clinical applicable polymeric composite scaffolds for auricular cartilage tissue engineering requires appropriate mechanical strength and biological characteristics.
34052269	4	33	theme	chondrogenic	909:920	arg1	potential					922:930	chondrogenic potential	909:930	chondrogenic potential	909:930	Furthermore, in vitro evaluation of chondrogenic potential showed that the MAF provided better cell adhesion, viability, proliferation and GAG secretion than the MBF.
34052269	3	34	theme	fiber-based	821:831	arg1	scaffolds					833:841	the Bm silk fiber-based scaffolds	809:841	the Bm silk fiber-based scaffolds reinforced by PLLA PMs (MBF)	809:870	We found that the Ap silk fiber-based scaffolds reinforced by PLLA PMs (MAF) exhibited superior physical properties (the mechanical properties in particular) as compared to the Bm silk fiber-based scaffolds reinforced by PLLA PMs (MBF).
34052269	2	35	theme	Antheraea	451:459	arg1	Ap					469:470	Ap	469:470	Ap	469:470	In this study, silk fiber-based scaffolds co-reinforced with poly-L-lactic acid porous microspheres (PLLA PMs) combined with either Bombyx mori (Bm) or Antheraea pernyi (Ap) silk fibers were fabricated as inspired by the "steel bars reinforced concrete" structure in architecture and their chondrogenic functions were also investigated.
34052269	2	35	theme	Antheraea	451:459	arg1	pernyi					461:466	Antheraea pernyi	451:466	Antheraea pernyi (Ap)	451:471	In this study, silk fiber-based scaffolds co-reinforced with poly-L-lactic acid porous microspheres (PLLA PMs) combined with either Bombyx mori (Bm) or Antheraea pernyi (Ap) silk fibers were fabricated as inspired by the "steel bars reinforced concrete" structure in architecture and their chondrogenic functions were also investigated.
34052269	0	36	theme	fiber-based	18:28	arg1	scaffolds					30:38	Non-mulberry silk fiber-based scaffolds	0:38	Non-mulberry silk fiber-based scaffolds reinforced by PLLA porous microspheres for auricular cartilage	0:101	Non-mulberry silk fiber-based scaffolds reinforced by PLLA porous microspheres for auricular cartilage: An in vitro study.
34052269	3	37	theme	silk	657:660	arg1	scaffolds					674:682	the Ap silk fiber-based scaffolds	650:682	the Ap silk fiber-based scaffolds reinforced by PLLA PMs (MAF)	650:711	We found that the Ap silk fiber-based scaffolds reinforced by PLLA PMs (MAF) exhibited superior physical properties (the mechanical properties in particular) as compared to the Bm silk fiber-based scaffolds reinforced by PLLA PMs (MBF).
34052269	2	38	theme	"	551:551	arg1	structure					553:561	concrete" structure	543:561	concrete" structure in architecture	543:577	In this study, silk fiber-based scaffolds co-reinforced with poly-L-lactic acid porous microspheres (PLLA PMs) combined with either Bombyx mori (Bm) or Antheraea pernyi (Ap) silk fibers were fabricated as inspired by the "steel bars reinforced concrete" structure in architecture and their chondrogenic functions were also investigated.
34052269	3	39	theme	fiber-based	662:672	arg1	scaffolds					674:682	the Ap silk fiber-based scaffolds	650:682	the Ap silk fiber-based scaffolds reinforced by PLLA PMs (MAF)	650:711	We found that the Ap silk fiber-based scaffolds reinforced by PLLA PMs (MAF) exhibited superior physical properties (the mechanical properties in particular) as compared to the Bm silk fiber-based scaffolds reinforced by PLLA PMs (MBF).
34052269	4	40	theme	potential	922:930	arg1	evaluation					895:904	in vitro evaluation	886:904	in vitro evaluation of chondrogenic potential	886:930	Furthermore, in vitro evaluation of chondrogenic potential showed that the MAF provided better cell adhesion, viability, proliferation and GAG secretion than the MBF.
34052269	1	41	theme	appropriate	235:245	arg1	strength					258:265	appropriate mechanical strength	235:265	appropriate mechanical strength	235:265	Designing clinical applicable polymeric composite scaffolds for auricular cartilage tissue engineering requires appropriate mechanical strength and biological characteristics.
34052269	5	42	theme	surgery-related	1136:1150	arg1	applications					1152:1163	relevant plastic surgery-related applications	1119:1163	relevant plastic surgery-related applications	1119:1163	Therefore, the MAF are promising in auricular cartilage tissue engineering and relevant plastic surgery-related applications.
34052269	2	43	theme	concrete	543:550	arg1	structure					553:561	concrete" structure	543:561	concrete" structure in architecture	543:577	In this study, silk fiber-based scaffolds co-reinforced with poly-L-lactic acid porous microspheres (PLLA PMs) combined with either Bombyx mori (Bm) or Antheraea pernyi (Ap) silk fibers were fabricated as inspired by the "steel bars reinforced concrete" structure in architecture and their chondrogenic functions were also investigated.
34052269	1	44	theme	mechanical	247:256	arg1	strength					258:265	appropriate mechanical strength	235:265	appropriate mechanical strength	235:265	Designing clinical applicable polymeric composite scaffolds for auricular cartilage tissue engineering requires appropriate mechanical strength and biological characteristics.
34052269	0	45	theme	porous	59:64	arg1	microspheres					66:77	PLLA porous microspheres	54:77	PLLA porous microspheres for auricular cartilage	54:101	Non-mulberry silk fiber-based scaffolds reinforced by PLLA porous microspheres for auricular cartilage: An in vitro study.
34052269	2	46	theme	chondrogenic	589:600	arg1	functions					602:610	their chondrogenic functions	583:610	their chondrogenic functions	583:610	In this study, silk fiber-based scaffolds co-reinforced with poly-L-lactic acid porous microspheres (PLLA PMs) combined with either Bombyx mori (Bm) or Antheraea pernyi (Ap) silk fibers were fabricated as inspired by the "steel bars reinforced concrete" structure in architecture and their chondrogenic functions were also investigated.
34052269	3	47	theme	Ap	654:655	arg1	scaffolds					674:682	the Ap silk fiber-based scaffolds	650:682	the Ap silk fiber-based scaffolds reinforced by PLLA PMs (MAF)	650:711	We found that the Ap silk fiber-based scaffolds reinforced by PLLA PMs (MAF) exhibited superior physical properties (the mechanical properties in particular) as compared to the Bm silk fiber-based scaffolds reinforced by PLLA PMs (MBF).
34052269	2	48	theme	PLLA	400:403	arg1	microspheres					386:397	poly-L-lactic acid porous microspheres	360:397	poly-L-lactic acid porous microspheres (PLLA PMs) combined with either Bombyx mori (Bm) or Antheraea pernyi (Ap) silk fibers	360:483	In this study, silk fiber-based scaffolds co-reinforced with poly-L-lactic acid porous microspheres (PLLA PMs) combined with either Bombyx mori (Bm) or Antheraea pernyi (Ap) silk fibers were fabricated as inspired by the "steel bars reinforced concrete" structure in architecture and their chondrogenic functions were also investigated.
34052269	2	48	theme	PLLA	400:403	arg1	PMs					405:407	PLLA PMs	400:407	PLLA PMs	400:407	In this study, silk fiber-based scaffolds co-reinforced with poly-L-lactic acid porous microspheres (PLLA PMs) combined with either Bombyx mori (Bm) or Antheraea pernyi (Ap) silk fibers were fabricated as inspired by the "steel bars reinforced concrete" structure in architecture and their chondrogenic functions were also investigated.
34052269	0	49	theme	PLLA	54:57	arg1	microspheres					66:77	PLLA porous microspheres	54:77	PLLA porous microspheres for auricular cartilage	54:101	Non-mulberry silk fiber-based scaffolds reinforced by PLLA porous microspheres for auricular cartilage: An in vitro study.
34052269	5	50	from	engineering	1103:1113	arg1	MAF					1055:1057	the MAF	1051:1057	the MAF	1051:1057	Therefore, the MAF are promising in auricular cartilage tissue engineering and relevant plastic surgery-related applications.
34052269	5	50	from	engineering	1103:1113	arg1	promising					1063:1071	promising	1063:1071	promising	1063:1071	Therefore, the MAF are promising in auricular cartilage tissue engineering and relevant plastic surgery-related applications.
34052269	2	51	theme	silk	473:476	arg1	fibers					478:483	silk fibers	473:483	silk fibers	473:483	In this study, silk fiber-based scaffolds co-reinforced with poly-L-lactic acid porous microspheres (PLLA PMs) combined with either Bombyx mori (Bm) or Antheraea pernyi (Ap) silk fibers were fabricated as inspired by the "steel bars reinforced concrete" structure in architecture and their chondrogenic functions were also investigated.
34052269	3	52	dep	properties	741:750	arg1	properties					768:777	the mechanical properties	753:777	the mechanical properties in particular	753:791	We found that the Ap silk fiber-based scaffolds reinforced by PLLA PMs (MAF) exhibited superior physical properties (the mechanical properties in particular) as compared to the Bm silk fiber-based scaffolds reinforced by PLLA PMs (MBF).
34052269	3	53	theme	PLLA	698:701	arg1	MAF					708:710	PLLA PMs (MAF)	698:711	PLLA PMs (MAF)	698:711	We found that the Ap silk fiber-based scaffolds reinforced by PLLA PMs (MAF) exhibited superior physical properties (the mechanical properties in particular) as compared to the Bm silk fiber-based scaffolds reinforced by PLLA PMs (MBF).
34052269	1	54	theme	clinical	133:140	arg1	scaffolds					173:181	clinical applicable polymeric composite scaffolds	133:181	clinical applicable polymeric composite scaffolds	133:181	Designing clinical applicable polymeric composite scaffolds for auricular cartilage tissue engineering requires appropriate mechanical strength and biological characteristics.
34052269	3	55	theme	Bm	813:814	arg1	scaffolds					833:841	the Bm silk fiber-based scaffolds	809:841	the Bm silk fiber-based scaffolds reinforced by PLLA PMs (MBF)	809:870	We found that the Ap silk fiber-based scaffolds reinforced by PLLA PMs (MAF) exhibited superior physical properties (the mechanical properties in particular) as compared to the Bm silk fiber-based scaffolds reinforced by PLLA PMs (MBF).
34052269	4	56	theme	cell	968:971	arg1	adhesion					973:980	better cell adhesion	961:980	better cell adhesion	961:980	Furthermore, in vitro evaluation of chondrogenic potential showed that the MAF provided better cell adhesion, viability, proliferation and GAG secretion than the MBF.
34052269	1	57	theme	applicable	142:151	arg1	scaffolds					173:181	clinical applicable polymeric composite scaffolds	133:181	clinical applicable polymeric composite scaffolds	133:181	Designing clinical applicable polymeric composite scaffolds for auricular cartilage tissue engineering requires appropriate mechanical strength and biological characteristics.
34052269	3	58	theme	PLLA	857:860	arg1	PMs					862:864	PLLA PMs	857:864	PLLA PMs (MBF)	857:870	We found that the Ap silk fiber-based scaffolds reinforced by PLLA PMs (MAF) exhibited superior physical properties (the mechanical properties in particular) as compared to the Bm silk fiber-based scaffolds reinforced by PLLA PMs (MBF).
34052269	3	58	theme	PLLA	857:860	arg1	MBF					867:869	MBF	867:869	MBF	867:869	We found that the Ap silk fiber-based scaffolds reinforced by PLLA PMs (MAF) exhibited superior physical properties (the mechanical properties in particular) as compared to the Bm silk fiber-based scaffolds reinforced by PLLA PMs (MBF).
34052269	1	59	theme	biological	271:280	arg1	characteristics					282:296	biological characteristics	271:296	biological characteristics	271:296	Designing clinical applicable polymeric composite scaffolds for auricular cartilage tissue engineering requires appropriate mechanical strength and biological characteristics.
34052269	4	60	theme	better	961:966	arg1	adhesion					973:980	better cell adhesion	961:980	better cell adhesion	961:980	Furthermore, in vitro evaluation of chondrogenic potential showed that the MAF provided better cell adhesion, viability, proliferation and GAG secretion than the MBF.
34052269	2	61	dep	mori	438:441	arg1	fibers					478:483	silk fibers	473:483	silk fibers	473:483	In this study, silk fiber-based scaffolds co-reinforced with poly-L-lactic acid porous microspheres (PLLA PMs) combined with either Bombyx mori (Bm) or Antheraea pernyi (Ap) silk fibers were fabricated as inspired by the "steel bars reinforced concrete" structure in architecture and their chondrogenic functions were also investigated.
34052269	1	62	theme	polymeric	153:161	arg1	scaffolds					173:181	clinical applicable polymeric composite scaffolds	133:181	clinical applicable polymeric composite scaffolds	133:181	Designing clinical applicable polymeric composite scaffolds for auricular cartilage tissue engineering requires appropriate mechanical strength and biological characteristics.
34052269	2	63	from	structure	553:561	arg1	architecture					566:577	architecture	566:577	architecture	566:577	In this study, silk fiber-based scaffolds co-reinforced with poly-L-lactic acid porous microspheres (PLLA PMs) combined with either Bombyx mori (Bm) or Antheraea pernyi (Ap) silk fibers were fabricated as inspired by the "steel bars reinforced concrete" structure in architecture and their chondrogenic functions were also investigated.
32380646	8	0	theme	low	1039:1041	arg1	expression					1043:1052	The low expression	1035:1052	The low expression of some DEGs (e.g., SUSase, ADPase, Pho1, Waxy, SBE, SSI, and SS II a)	1035:1123	The low expression of some DEGs (e.g., SUSase, ADPase, Pho1, Waxy, SBE, SSI, and SS II a) might explain the reduction of starch contents.
32380646	10	1	theme	reporter	1338:1345	arg1	assay					1347:1351	dual-luciferase reporter assay	1322:1351	dual-luciferase reporter assay	1322:1351	In addition, dual-luciferase reporter assay indicated that both TaSPA-B and TaPBF could transactivate the promoter of ω-1,2 gliadin gene.
32380646	2	2	theme	protein	345:351	arg1	biosynthesis					353:364	starch and protein biosynthesis	334:364	starch and protein biosynthesis in wheat	334:373	Several transcription factors (TFs) regulate gene expression during starch and protein biosynthesis in wheat.
32380646	11	3	theme	complex	1494:1500	arg1	network					1507:1513	a complex gene network	1492:1513	a complex gene network	1492:1513	These results suggest that TaSPA-B regulates a complex gene network and plays an important role in starch and protein biosynthesis in wheat.
32380646	9	4	theme	OE-3	1297:1300	arg1	lines					1302:1306	TaSPA-B OE-3 lines	1289:1306	TaSPA-B OE-3 lines	1289:1306	Some TFs involved in glutenin and starch synthesis might be regulated by TaSPA-B, for example, TaPBF was reduced in TaSPA-B OE-3 lines.
32380646	6	5	theme	gliadin	948:954	arg1	gene					956:959	ω-1,2 gliadin gene	942:959	ω-1,2 gliadin gene	942:959	Moreover, glutenin and ω- gliadin contents were significantly reduced, with lower expression levels of related genes (e.g., By15, Dx2, and ω-1,2 gliadin gene).
32380646	6	6	theme	gliadin	829:835	arg1	contents					837:844	glutenin and ω- gliadin contents	813:844	contents	837:844	Moreover, glutenin and ω- gliadin contents were significantly reduced, with lower expression levels of related genes (e.g., By15, Dx2, and ω-1,2 gliadin gene).
32380646	1	7	theme	important	153:161	arg1	content					141:147	content	141:147	content	141:147	Starch and prolamin composition and content are important indexes for determining the processing and nutritional quality of wheat (Triticum aestivum L.) grains.
32380646	1	7	theme	important	153:161	arg1	indexes					163:169	important indexes	153:169	important indexes	153:169	Starch and prolamin composition and content are important indexes for determining the processing and nutritional quality of wheat (Triticum aestivum L.) grains.
32380646	1	7	theme	important	153:161	arg1	composition					125:135	Starch and prolamin composition	105:135	Starch and prolamin composition	105:135	Starch and prolamin composition and content are important indexes for determining the processing and nutritional quality of wheat (Triticum aestivum L.) grains.
32380646	11	8	theme	gene	1502:1505	arg1	network					1507:1513	a complex gene network	1492:1513	a complex gene network	1492:1513	These results suggest that TaSPA-B regulates a complex gene network and plays an important role in starch and protein biosynthesis in wheat.
32380646	6	9	theme	ω-	826:827	arg1	contents					837:844	glutenin and ω- gliadin contents	813:844	contents	837:844	Moreover, glutenin and ω- gliadin contents were significantly reduced, with lower expression levels of related genes (e.g., By15, Dx2, and ω-1,2 gliadin gene).
32380646	7	10	theme	expressed	1011:1019	arg1	genes					1021:1025	2023 differentially expressed genes	991:1025	2023 differentially expressed genes (DEGs)	991:1032	RNA-seq analysis identified 2023 differentially expressed genes (DEGs).
32380646	7	10	theme	expressed	1011:1019	arg1	DEGs					1028:1031	DEGs	1028:1031	DEGs	1028:1031	RNA-seq analysis identified 2023 differentially expressed genes (DEGs).
32380646	3	11	theme	glutenin	493:500	arg1	genes					502:506	glutenin genes	493:506	glutenin genes	493:506	Storage protein activator (TaSPA), a member of the basic leucine zipper (bZIP) family, has been reported to activate glutenin genes and is correlated to starch synthesis related genes.
32380646	6	12	theme	genes	914:918	arg1	levels					896:901	lower expression levels	879:901	lower expression levels of related genes (e.g., By15, Dx2, and ω-1,2 gliadin gene)	879:960	Moreover, glutenin and ω- gliadin contents were significantly reduced, with lower expression levels of related genes (e.g., By15, Dx2, and ω-1,2 gliadin gene).
32380646	6	13	dep	By15	927:930	arg1	e.g.					921:924	e.g.	921:924	e.g.	921:924	Moreover, glutenin and ω- gliadin contents were significantly reduced, with lower expression levels of related genes (e.g., By15, Dx2, and ω-1,2 gliadin gene).
32380646	2	14	theme	transcription	274:286	arg1	TFs					297:299	TFs	297:299	TFs	297:299	Several transcription factors (TFs) regulate gene expression during starch and protein biosynthesis in wheat.
32380646	2	14	theme	transcription	274:286	arg1	factors					288:294	Several transcription factors	266:294	Several transcription factors (TFs)	266:300	Several transcription factors (TFs) regulate gene expression during starch and protein biosynthesis in wheat.
32380646	1	15	theme	grains	258:263	arg1	processing					191:200	processing	191:200	processing	191:200	Starch and prolamin composition and content are important indexes for determining the processing and nutritional quality of wheat (Triticum aestivum L.) grains.
32380646	1	15	theme	grains	258:263	arg1	quality					218:224	nutritional quality	206:224	nutritional quality	206:224	Starch and prolamin composition and content are important indexes for determining the processing and nutritional quality of wheat (Triticum aestivum L.) grains.
32380646	8	16	theme	DEGs	1062:1065	arg1	expression					1043:1052	The low expression	1035:1052	The low expression of some DEGs (e.g., SUSase, ADPase, Pho1, Waxy, SBE, SSI, and SS II a)	1035:1123	The low expression of some DEGs (e.g., SUSase, ADPase, Pho1, Waxy, SBE, SSI, and SS II a) might explain the reduction of starch contents.
32380646	1	17	theme	Starch	105:110	arg1	content					141:147	content	141:147	content	141:147	Starch and prolamin composition and content are important indexes for determining the processing and nutritional quality of wheat (Triticum aestivum L.) grains.
32380646	1	17	theme	Starch	105:110	arg1	indexes					163:169	important indexes	153:169	important indexes	153:169	Starch and prolamin composition and content are important indexes for determining the processing and nutritional quality of wheat (Triticum aestivum L.) grains.
32380646	1	17	theme	Starch	105:110	arg1	composition					125:135	Starch and prolamin composition	105:135	Starch and prolamin composition	105:135	Starch and prolamin composition and content are important indexes for determining the processing and nutritional quality of wheat (Triticum aestivum L.) grains.
32380646	6	18	theme	ω-1,2	942:946	arg1	gene					956:959	ω-1,2 gliadin gene	942:959	ω-1,2 gliadin gene	942:959	Moreover, glutenin and ω- gliadin contents were significantly reduced, with lower expression levels of related genes (e.g., By15, Dx2, and ω-1,2 gliadin gene).
32380646	2	19	theme	Several	266:272	arg1	TFs					297:299	TFs	297:299	TFs	297:299	Several transcription factors (TFs) regulate gene expression during starch and protein biosynthesis in wheat.
32380646	2	19	theme	Several	266:272	arg1	factors					288:294	Several transcription factors	266:294	Several transcription factors (TFs)	266:300	Several transcription factors (TFs) regulate gene expression during starch and protein biosynthesis in wheat.
32380646	5	20	theme	TaSPA-B	785:791	arg1	lines					796:800	the TaSPA-B OE lines	781:800	the TaSPA-B OE lines	781:800	Compared with wild-type (WT) plants, the starch content was slightly reduced and starch granules exhibited a more polarized distribution in the TaSPA-B OE lines.
32380646	6	21	theme	related	906:912	arg1	genes					914:918	related genes	906:918	related genes (e.g., By15, Dx2, and ω-1,2 gliadin gene)	906:960	Moreover, glutenin and ω- gliadin contents were significantly reduced, with lower expression levels of related genes (e.g., By15, Dx2, and ω-1,2 gliadin gene).
32380646	10	22	theme	gliadin	1433:1439	arg1	gene					1441:1444	ω-1,2 gliadin gene	1427:1444	ω-1,2 gliadin gene	1427:1444	In addition, dual-luciferase reporter assay indicated that both TaSPA-B and TaPBF could transactivate the promoter of ω-1,2 gliadin gene.
32380646	3	23	theme	protein	384:390	arg1	member					413:418	a member	411:418	a member of the basic leucine zipper (bZIP) family	411:460	Storage protein activator (TaSPA), a member of the basic leucine zipper (bZIP) family, has been reported to activate glutenin genes and is correlated to starch synthesis related genes.
32380646	3	23	theme	protein	384:390	arg1	TaSPA					403:407	TaSPA	403:407	TaSPA	403:407	Storage protein activator (TaSPA), a member of the basic leucine zipper (bZIP) family, has been reported to activate glutenin genes and is correlated to starch synthesis related genes.
32380646	3	23	theme	protein	384:390	arg1	activator					392:400	Storage protein activator	376:400	Storage protein activator (TaSPA)	376:408	Storage protein activator (TaSPA), a member of the basic leucine zipper (bZIP) family, has been reported to activate glutenin genes and is correlated to starch synthesis related genes.
32380646	4	24	theme	overexpressing	597:610	arg1	lines					634:638	TaSPA-B overexpressing (OE) transgenic wheat lines	589:638	TaSPA-B overexpressing (OE) transgenic wheat lines	589:638	In this study, we generated TaSPA-B overexpressing (OE) transgenic wheat lines.
32380646	3	25	theme	Storage	376:382	arg1	member					413:418	a member	411:418	a member of the basic leucine zipper (bZIP) family	411:460	Storage protein activator (TaSPA), a member of the basic leucine zipper (bZIP) family, has been reported to activate glutenin genes and is correlated to starch synthesis related genes.
32380646	3	25	theme	Storage	376:382	arg1	TaSPA					403:407	TaSPA	403:407	TaSPA	403:407	Storage protein activator (TaSPA), a member of the basic leucine zipper (bZIP) family, has been reported to activate glutenin genes and is correlated to starch synthesis related genes.
32380646	3	25	theme	Storage	376:382	arg1	activator					392:400	Storage protein activator	376:400	Storage protein activator (TaSPA)	376:408	Storage protein activator (TaSPA), a member of the basic leucine zipper (bZIP) family, has been reported to activate glutenin genes and is correlated to starch synthesis related genes.
32380646	10	26	theme	dual-luciferase	1322:1336	arg1	assay					1347:1351	dual-luciferase reporter assay	1322:1351	dual-luciferase reporter assay	1322:1351	In addition, dual-luciferase reporter assay indicated that both TaSPA-B and TaPBF could transactivate the promoter of ω-1,2 gliadin gene.
32380646	6	27	theme	glutenin	813:820	arg1	contents					837:844	glutenin and ω- gliadin contents	813:844	contents	837:844	Moreover, glutenin and ω- gliadin contents were significantly reduced, with lower expression levels of related genes (e.g., By15, Dx2, and ω-1,2 gliadin gene).
32380646	0	28	from	Accumulation	52:63	arg1	Triticum					75:82	Triticum	75:82	Triticum	75:82	Over-Expressing TaSPA-B Reduces Prolamin and Starch Accumulation in Wheat (Triticum aestivum L.) Grains.
32380646	0	28	from	Accumulation	52:63	arg1	Grains					97:102	Wheat (Triticum aestivum L.) Grains	68:102	Wheat (Triticum aestivum L.) Grains	68:102	Over-Expressing TaSPA-B Reduces Prolamin and Starch Accumulation in Wheat (Triticum aestivum L.) Grains.
32380646	4	29	theme	TaSPA-B	589:595	arg1	lines					634:638	TaSPA-B overexpressing (OE) transgenic wheat lines	589:638	TaSPA-B overexpressing (OE) transgenic wheat lines	589:638	In this study, we generated TaSPA-B overexpressing (OE) transgenic wheat lines.
32380646	0	30	theme	Over-Expressing	0:14	arg1	TaSPA-B					16:22	Over-Expressing TaSPA-B	0:22	Over-Expressing TaSPA-B	0:22	Over-Expressing TaSPA-B Reduces Prolamin and Starch Accumulation in Wheat (Triticum aestivum L.) Grains.
32380646	0	31	dep	Triticum	75:82	arg1	L.					93:94	Triticum aestivum L.	75:94	Triticum aestivum L.	75:94	Over-Expressing TaSPA-B Reduces Prolamin and Starch Accumulation in Wheat (Triticum aestivum L.) Grains.
32380646	4	32	theme	OE	613:614	arg1	lines					634:638	TaSPA-B overexpressing (OE) transgenic wheat lines	589:638	TaSPA-B overexpressing (OE) transgenic wheat lines	589:638	In this study, we generated TaSPA-B overexpressing (OE) transgenic wheat lines.
32380646	2	33	from	biosynthesis	353:364	arg1	wheat					369:373	wheat	369:373	wheat	369:373	Several transcription factors (TFs) regulate gene expression during starch and protein biosynthesis in wheat.
32380646	3	34	theme	leucine	433:439	arg1	bZIP					449:452	bZIP	449:452	bZIP	449:452	Storage protein activator (TaSPA), a member of the basic leucine zipper (bZIP) family, has been reported to activate glutenin genes and is correlated to starch synthesis related genes.
32380646	3	34	theme	leucine	433:439	arg1	zipper					441:446	basic leucine zipper	427:446	the basic leucine zipper (bZIP) family	423:460	Storage protein activator (TaSPA), a member of the basic leucine zipper (bZIP) family, has been reported to activate glutenin genes and is correlated to starch synthesis related genes.
32380646	6	35	theme	expression	885:894	arg1	levels					896:901	lower expression levels	879:901	lower expression levels of related genes (e.g., By15, Dx2, and ω-1,2 gliadin gene)	879:960	Moreover, glutenin and ω- gliadin contents were significantly reduced, with lower expression levels of related genes (e.g., By15, Dx2, and ω-1,2 gliadin gene).
32380646	7	36	theme	RNA-seq	963:969	arg1	analysis					971:978	RNA-seq analysis	963:978	RNA-seq analysis	963:978	RNA-seq analysis identified 2023 differentially expressed genes (DEGs).
32380646	11	37	theme	protein	1557:1563	arg1	biosynthesis					1565:1576	starch and protein biosynthesis	1546:1576	starch and protein biosynthesis in wheat	1546:1585	These results suggest that TaSPA-B regulates a complex gene network and plays an important role in starch and protein biosynthesis in wheat.
32380646	3	38	theme	zipper	441:446	arg1	family					455:460	the basic leucine zipper (bZIP) family	423:460	the basic leucine zipper (bZIP) family	423:460	Storage protein activator (TaSPA), a member of the basic leucine zipper (bZIP) family, has been reported to activate glutenin genes and is correlated to starch synthesis related genes.
32380646	0	39	theme	Starch	45:50	arg1	Accumulation					52:63	Starch Accumulation	45:63	Starch Accumulation	45:63	Over-Expressing TaSPA-B Reduces Prolamin and Starch Accumulation in Wheat (Triticum aestivum L.) Grains.
32380646	6	40	theme	lower	879:883	arg1	levels					896:901	lower expression levels	879:901	lower expression levels of related genes (e.g., By15, Dx2, and ω-1,2 gliadin gene)	879:960	Moreover, glutenin and ω- gliadin contents were significantly reduced, with lower expression levels of related genes (e.g., By15, Dx2, and ω-1,2 gliadin gene).
32380646	5	41	theme	wild-type	655:663	arg1	plants					670:675	wild-type (WT) plants	655:675	wild-type (WT) plants	655:675	Compared with wild-type (WT) plants, the starch content was slightly reduced and starch granules exhibited a more polarized distribution in the TaSPA-B OE lines.
32380646	1	42	dep	Triticum	236:243	arg1	L.					254:255	Triticum aestivum L.	236:255	Triticum aestivum L.	236:255	Starch and prolamin composition and content are important indexes for determining the processing and nutritional quality of wheat (Triticum aestivum L.) grains.
32380646	3	43	theme	related	546:552	arg1	genes					554:558	starch synthesis related genes	529:558	starch synthesis related genes	529:558	Storage protein activator (TaSPA), a member of the basic leucine zipper (bZIP) family, has been reported to activate glutenin genes and is correlated to starch synthesis related genes.
32380646	10	44	theme	gene	1441:1444	arg1	promoter					1415:1422	the promoter	1411:1422	the promoter of ω-1,2 gliadin gene	1411:1444	In addition, dual-luciferase reporter assay indicated that both TaSPA-B and TaPBF could transactivate the promoter of ω-1,2 gliadin gene.
32380646	1	45	theme	nutritional	206:216	arg1	quality					218:224	nutritional quality	206:224	nutritional quality	206:224	Starch and prolamin composition and content are important indexes for determining the processing and nutritional quality of wheat (Triticum aestivum L.) grains.
32380646	10	46	theme	ω-1,2	1427:1431	arg1	gene					1441:1444	ω-1,2 gliadin gene	1427:1444	ω-1,2 gliadin gene	1427:1444	In addition, dual-luciferase reporter assay indicated that both TaSPA-B and TaPBF could transactivate the promoter of ω-1,2 gliadin gene.
32380646	3	47	theme	basic	427:431	arg1	bZIP					449:452	bZIP	449:452	bZIP	449:452	Storage protein activator (TaSPA), a member of the basic leucine zipper (bZIP) family, has been reported to activate glutenin genes and is correlated to starch synthesis related genes.
32380646	3	47	theme	basic	427:431	arg1	zipper					441:446	basic leucine zipper	427:446	the basic leucine zipper (bZIP) family	423:460	Storage protein activator (TaSPA), a member of the basic leucine zipper (bZIP) family, has been reported to activate glutenin genes and is correlated to starch synthesis related genes.
32380646	8	48	theme	II	1119:1120	arg1	a					1122:1122	SS II a	1116:1122	SS II a	1116:1122	The low expression of some DEGs (e.g., SUSase, ADPase, Pho1, Waxy, SBE, SSI, and SS II a) might explain the reduction of starch contents.
32380646	8	49	dep	SUSase	1074:1079	arg1	e.g.					1068:1071	e.g.	1068:1071	e.g.	1068:1071	The low expression of some DEGs (e.g., SUSase, ADPase, Pho1, Waxy, SBE, SSI, and SS II a) might explain the reduction of starch contents.
32380646	5	50	theme	starch	682:687	arg1	content					689:695	the starch content	678:695	the starch content	678:695	Compared with wild-type (WT) plants, the starch content was slightly reduced and starch granules exhibited a more polarized distribution in the TaSPA-B OE lines.
32380646	3	51	theme	starch	529:534	arg1	genes					554:558	starch synthesis related genes	529:558	starch synthesis related genes	529:558	Storage protein activator (TaSPA), a member of the basic leucine zipper (bZIP) family, has been reported to activate glutenin genes and is correlated to starch synthesis related genes.
32380646	2	52	theme	starch	334:339	arg1	biosynthesis					353:364	starch and protein biosynthesis	334:364	starch and protein biosynthesis in wheat	334:373	Several transcription factors (TFs) regulate gene expression during starch and protein biosynthesis in wheat.
32380646	5	53	theme	starch	722:727	arg1	granules					729:736	starch granules	722:736	starch granules	722:736	Compared with wild-type (WT) plants, the starch content was slightly reduced and starch granules exhibited a more polarized distribution in the TaSPA-B OE lines.
32380646	3	54	theme	synthesis	536:544	arg1	genes					554:558	starch synthesis related genes	529:558	starch synthesis related genes	529:558	Storage protein activator (TaSPA), a member of the basic leucine zipper (bZIP) family, has been reported to activate glutenin genes and is correlated to starch synthesis related genes.
32380646	3	55	theme	family	455:460	arg1	member					413:418	a member	411:418	a member of the basic leucine zipper (bZIP) family	411:460	Storage protein activator (TaSPA), a member of the basic leucine zipper (bZIP) family, has been reported to activate glutenin genes and is correlated to starch synthesis related genes.
32380646	3	55	theme	family	455:460	arg1	activator					392:400	Storage protein activator	376:400	Storage protein activator (TaSPA)	376:408	Storage protein activator (TaSPA), a member of the basic leucine zipper (bZIP) family, has been reported to activate glutenin genes and is correlated to starch synthesis related genes.
32380646	11	56	theme	important	1528:1536	arg1	role					1538:1541	an important role	1525:1541	an important role	1525:1541	These results suggest that TaSPA-B regulates a complex gene network and plays an important role in starch and protein biosynthesis in wheat.
32380646	5	57	from	distribution	765:776	arg1	lines					796:800	the TaSPA-B OE lines	781:800	the TaSPA-B OE lines	781:800	Compared with wild-type (WT) plants, the starch content was slightly reduced and starch granules exhibited a more polarized distribution in the TaSPA-B OE lines.
32380646	1	58	theme	prolamin	116:123	arg1	content					141:147	content	141:147	content	141:147	Starch and prolamin composition and content are important indexes for determining the processing and nutritional quality of wheat (Triticum aestivum L.) grains.
32380646	1	58	theme	prolamin	116:123	arg1	indexes					163:169	important indexes	153:169	important indexes	153:169	Starch and prolamin composition and content are important indexes for determining the processing and nutritional quality of wheat (Triticum aestivum L.) grains.
32380646	1	58	theme	prolamin	116:123	arg1	composition					125:135	Starch and prolamin composition	105:135	Starch and prolamin composition	105:135	Starch and prolamin composition and content are important indexes for determining the processing and nutritional quality of wheat (Triticum aestivum L.) grains.
32380646	11	59	theme	starch	1546:1551	arg1	biosynthesis					1565:1576	starch and protein biosynthesis	1546:1576	starch and protein biosynthesis in wheat	1546:1585	These results suggest that TaSPA-B regulates a complex gene network and plays an important role in starch and protein biosynthesis in wheat.
32380646	8	60	theme	contents	1163:1170	arg1	reduction					1143:1151	the reduction	1139:1151	the reduction of starch contents	1139:1170	The low expression of some DEGs (e.g., SUSase, ADPase, Pho1, Waxy, SBE, SSI, and SS II a) might explain the reduction of starch contents.
32380646	8	61	theme	starch	1156:1161	arg1	contents					1163:1170	starch contents	1156:1170	starch contents	1156:1170	The low expression of some DEGs (e.g., SUSase, ADPase, Pho1, Waxy, SBE, SSI, and SS II a) might explain the reduction of starch contents.
32380646	2	62	theme	gene	311:314	arg1	expression					316:325	gene expression	311:325	gene expression	311:325	Several transcription factors (TFs) regulate gene expression during starch and protein biosynthesis in wheat.
32380646	1	63	theme	wheat	229:233	arg1	grains					258:263	wheat (Triticum aestivum L.) grains	229:263	wheat (Triticum aestivum L.) grains	229:263	Starch and prolamin composition and content are important indexes for determining the processing and nutritional quality of wheat (Triticum aestivum L.) grains.
32380646	5	64	dep	wild-type	655:663	arg1	WT					666:667	WT	666:667	WT	666:667	Compared with wild-type (WT) plants, the starch content was slightly reduced and starch granules exhibited a more polarized distribution in the TaSPA-B OE lines.
32380646	1	65	dep	processing	191:200	arg1	the					187:189	the	187:189	the	187:189	Starch and prolamin composition and content are important indexes for determining the processing and nutritional quality of wheat (Triticum aestivum L.) grains.
32380646	5	66	theme	polarized	755:763	arg1	distribution					765:776	a more polarized distribution	748:776	a more polarized distribution in the TaSPA-B OE lines	748:800	Compared with wild-type (WT) plants, the starch content was slightly reduced and starch granules exhibited a more polarized distribution in the TaSPA-B OE lines.
32380646	4	67	theme	wheat	628:632	arg1	lines					634:638	TaSPA-B overexpressing (OE) transgenic wheat lines	589:638	TaSPA-B overexpressing (OE) transgenic wheat lines	589:638	In this study, we generated TaSPA-B overexpressing (OE) transgenic wheat lines.
32380646	5	68	theme	OE	793:794	arg1	lines					796:800	the TaSPA-B OE lines	781:800	the TaSPA-B OE lines	781:800	Compared with wild-type (WT) plants, the starch content was slightly reduced and starch granules exhibited a more polarized distribution in the TaSPA-B OE lines.
32380646	9	69	theme	starch	1207:1212	arg1	synthesis					1214:1222	starch synthesis	1207:1222	starch synthesis	1207:1222	Some TFs involved in glutenin and starch synthesis might be regulated by TaSPA-B, for example, TaPBF was reduced in TaSPA-B OE-3 lines.
32380646	0	70	theme	Wheat	68:72	arg1	Triticum					75:82	Triticum	75:82	Triticum	75:82	Over-Expressing TaSPA-B Reduces Prolamin and Starch Accumulation in Wheat (Triticum aestivum L.) Grains.
32380646	0	70	theme	Wheat	68:72	arg1	Grains					97:102	Wheat (Triticum aestivum L.) Grains	68:102	Wheat (Triticum aestivum L.) Grains	68:102	Over-Expressing TaSPA-B Reduces Prolamin and Starch Accumulation in Wheat (Triticum aestivum L.) Grains.
32380646	0	71	from	Prolamin	32:39	arg1	Triticum					75:82	Triticum	75:82	Triticum	75:82	Over-Expressing TaSPA-B Reduces Prolamin and Starch Accumulation in Wheat (Triticum aestivum L.) Grains.
32380646	0	71	from	Prolamin	32:39	arg1	Grains					97:102	Wheat (Triticum aestivum L.) Grains	68:102	Wheat (Triticum aestivum L.) Grains	68:102	Over-Expressing TaSPA-B Reduces Prolamin and Starch Accumulation in Wheat (Triticum aestivum L.) Grains.
32380646	9	72	theme	TaSPA-B	1289:1295	arg1	lines					1302:1306	TaSPA-B OE-3 lines	1289:1306	TaSPA-B OE-3 lines	1289:1306	Some TFs involved in glutenin and starch synthesis might be regulated by TaSPA-B, for example, TaPBF was reduced in TaSPA-B OE-3 lines.
32380646	4	73	theme	transgenic	617:626	arg1	lines					634:638	TaSPA-B overexpressing (OE) transgenic wheat lines	589:638	TaSPA-B overexpressing (OE) transgenic wheat lines	589:638	In this study, we generated TaSPA-B overexpressing (OE) transgenic wheat lines.
32380646	8	74	theme	SS	1116:1117	arg1	a					1122:1122	SS II a	1116:1122	SS II a	1116:1122	The low expression of some DEGs (e.g., SUSase, ADPase, Pho1, Waxy, SBE, SSI, and SS II a) might explain the reduction of starch contents.
32380646	11	75	from	biosynthesis	1565:1576	arg1	wheat					1581:1585	wheat	1581:1585	wheat	1581:1585	These results suggest that TaSPA-B regulates a complex gene network and plays an important role in starch and protein biosynthesis in wheat.
32078996	5	0	theme	storage	826:832	arg1	days					818:821	the first days	808:821	the first days of storage	808:832	This induction was more pronounced during the first days of storage and associated with the application of mannitol rather than water, suggesting that mannitol negatively regulated the postharvest strawberry ripening.
32078996	4	1	from	application	634:644	arg1	strawberries					667:678	unripe strawberries	660:678	unripe strawberries	660:678	The application of sucrose in unripe strawberries resulted in the induction of ripening, which is dependent on ABA and its derivatives.
32078996	3	2	theme	chemical	595:602	arg1	composition					604:614	altered chemical composition	587:614	altered chemical composition	587:614	The results suggested that the postharvest ripening process was dependent on the signal triggered by ABA and differed from in vivo ripening, resulting in fruits with altered chemical composition and firmness.
32078996	3	3	theme	postharvest	452:462	arg1	dependent					485:493	dependent	485:493	dependent	485:493	The results suggested that the postharvest ripening process was dependent on the signal triggered by ABA and differed from in vivo ripening, resulting in fruits with altered chemical composition and firmness.
32078996	3	3	theme	postharvest	452:462	arg1	process					473:479	the postharvest ripening process	448:479	the postharvest ripening process	448:479	The results suggested that the postharvest ripening process was dependent on the signal triggered by ABA and differed from in vivo ripening, resulting in fruits with altered chemical composition and firmness.
32078996	6	4	theme	ABA	1040:1042	arg1	role					1032:1035	the role	1028:1035	the role of ABA and sucrose in the regulation of postharvest ripening of strawberry	1028:1110	These results provide further insights into the role of ABA and sucrose in the regulation of postharvest ripening of strawberry.
32078996	3	5	theme	ripening	464:471	arg1	dependent					485:493	dependent	485:493	dependent	485:493	The results suggested that the postharvest ripening process was dependent on the signal triggered by ABA and differed from in vivo ripening, resulting in fruits with altered chemical composition and firmness.
32078996	3	5	theme	ripening	464:471	arg1	process					473:479	the postharvest ripening process	448:479	the postharvest ripening process	448:479	The results suggested that the postharvest ripening process was dependent on the signal triggered by ABA and differed from in vivo ripening, resulting in fruits with altered chemical composition and firmness.
32078996	0	6	dep	in	102:103	arg1	vivo					105:108	vivo	105:108	vivo	105:108	The postharvest ripening of strawberry fruits induced by abscisic acid and sucrose differs from their in vivo ripening.
32078996	6	7	from	role	1032:1035	arg1	regulation					1063:1072	the regulation	1059:1072	the regulation of postharvest ripening of strawberry	1059:1110	These results provide further insights into the role of ABA and sucrose in the regulation of postharvest ripening of strawberry.
32078996	1	8	attach	attached	242:249	arg1	plant					254:258	plant	254:258	plant	254:258	In this study, we compared the chemical composition of strawberry (Fragaria × ananassa) fruits that were ripened in vivo (attached to plant) to those ripened during postharvest storage.
32078996	1	8	attach	attached	242:249	arg2	fruits					208:213	strawberry (Fragaria × ananassa) fruits	175:213	strawberry (Fragaria × ananassa) fruits that were ripened in vivo (attached to plant) to those ripened during postharvest storage	175:303	In this study, we compared the chemical composition of strawberry (Fragaria × ananassa) fruits that were ripened in vivo (attached to plant) to those ripened during postharvest storage.
32078996	0	9	theme	in	102:103	arg1	ripening					110:117	their in vivo ripening	96:117	their in vivo ripening	96:117	The postharvest ripening of strawberry fruits induced by abscisic acid and sucrose differs from their in vivo ripening.
32078996	1	10	theme	chemical	151:158	arg1	composition					160:170	the chemical composition	147:170	the chemical composition of strawberry (Fragaria × ananassa) fruits that were ripened in vivo (attached to plant) to those ripened during postharvest storage	147:303	In this study, we compared the chemical composition of strawberry (Fragaria × ananassa) fruits that were ripened in vivo (attached to plant) to those ripened during postharvest storage.
32078996	2	11	theme	acid	349:352	arg1	application					325:335	the application	321:335	the application of abscisic acid (ABA) and sucrose	321:370	The effects of the application of abscisic acid (ABA) and sucrose on the postharvest ripening were also evaluated.
32078996	0	12	theme	postharvest	4:14	arg1	ripening					16:23	The postharvest ripening	0:23	The postharvest ripening of strawberry fruits induced by abscisic acid and sucrose	0:81	The postharvest ripening of strawberry fruits induced by abscisic acid and sucrose differs from their in vivo ripening.
32078996	2	13	from	effects	310:316	arg1	ripening					391:398	the postharvest ripening	375:398	the postharvest ripening	375:398	The effects of the application of abscisic acid (ABA) and sucrose on the postharvest ripening were also evaluated.
32078996	6	14	theme	strawberry	1101:1110	arg1	ripening					1089:1096	postharvest ripening	1077:1096	postharvest ripening of strawberry	1077:1110	These results provide further insights into the role of ABA and sucrose in the regulation of postharvest ripening of strawberry.
32078996	2	15	theme	abscisic	340:347	arg1	acid					349:352	abscisic acid	340:352	abscisic acid (ABA)	340:358	The effects of the application of abscisic acid (ABA) and sucrose on the postharvest ripening were also evaluated.
32078996	2	15	theme	abscisic	340:347	arg1	ABA					355:357	ABA	355:357	ABA	355:357	The effects of the application of abscisic acid (ABA) and sucrose on the postharvest ripening were also evaluated.
32078996	2	16	theme	postharvest	379:389	arg1	ripening					391:398	the postharvest ripening	375:398	the postharvest ripening	375:398	The effects of the application of abscisic acid (ABA) and sucrose on the postharvest ripening were also evaluated.
32078996	5	17	theme	mannitol	873:880	arg1	application					858:868	the application	854:868	the application of mannitol rather than water	854:898	This induction was more pronounced during the first days of storage and associated with the application of mannitol rather than water, suggesting that mannitol negatively regulated the postharvest strawberry ripening.
32078996	3	18	dep	in	544:545	arg1	vivo					547:550	vivo	547:550	vivo	547:550	The results suggested that the postharvest ripening process was dependent on the signal triggered by ABA and differed from in vivo ripening, resulting in fruits with altered chemical composition and firmness.
32078996	6	19	theme	ripening	1089:1096	arg1	regulation					1063:1072	the regulation	1059:1072	the regulation of postharvest ripening of strawberry	1059:1110	These results provide further insights into the role of ABA and sucrose in the regulation of postharvest ripening of strawberry.
32078996	3	20	with	fruits	575:580	arg1	composition					604:614	altered chemical composition	587:614	altered chemical composition	587:614	The results suggested that the postharvest ripening process was dependent on the signal triggered by ABA and differed from in vivo ripening, resulting in fruits with altered chemical composition and firmness.
32078996	3	20	with	fruits	575:580	arg1	firmness					620:627	firmness	620:627	firmness	620:627	The results suggested that the postharvest ripening process was dependent on the signal triggered by ABA and differed from in vivo ripening, resulting in fruits with altered chemical composition and firmness.
32078996	2	21	theme	sucrose	364:370	arg1	application					325:335	the application	321:335	the application of abscisic acid (ABA) and sucrose	321:370	The effects of the application of abscisic acid (ABA) and sucrose on the postharvest ripening were also evaluated.
32078996	6	22	theme	postharvest	1077:1087	arg1	ripening					1089:1096	postharvest ripening	1077:1096	postharvest ripening of strawberry	1077:1110	These results provide further insights into the role of ABA and sucrose in the regulation of postharvest ripening of strawberry.
32078996	1	23	theme	strawberry	175:184	arg1	fruits					208:213	strawberry (Fragaria × ananassa) fruits	175:213	strawberry (Fragaria × ananassa) fruits that were ripened in vivo (attached to plant) to those ripened during postharvest storage	175:303	In this study, we compared the chemical composition of strawberry (Fragaria × ananassa) fruits that were ripened in vivo (attached to plant) to those ripened during postharvest storage.
32078996	0	24	theme	fruits	39:44	arg1	ripening					16:23	The postharvest ripening	0:23	The postharvest ripening of strawberry fruits induced by abscisic acid and sucrose	0:81	The postharvest ripening of strawberry fruits induced by abscisic acid and sucrose differs from their in vivo ripening.
32078996	3	25	theme	altered	587:593	arg1	composition					604:614	altered chemical composition	587:614	altered chemical composition	587:614	The results suggested that the postharvest ripening process was dependent on the signal triggered by ABA and differed from in vivo ripening, resulting in fruits with altered chemical composition and firmness.
32078996	5	26	theme	postharvest	951:961	arg1	ripening					974:981	the postharvest strawberry ripening	947:981	the postharvest strawberry ripening	947:981	This induction was more pronounced during the first days of storage and associated with the application of mannitol rather than water, suggesting that mannitol negatively regulated the postharvest strawberry ripening.
32078996	5	27	theme	first	812:816	arg1	days					818:821	the first days	808:821	the first days of storage	808:832	This induction was more pronounced during the first days of storage and associated with the application of mannitol rather than water, suggesting that mannitol negatively regulated the postharvest strawberry ripening.
32078996	0	28	theme	strawberry	28:37	arg1	fruits					39:44	strawberry fruits	28:44	strawberry fruits	28:44	The postharvest ripening of strawberry fruits induced by abscisic acid and sucrose differs from their in vivo ripening.
32078996	3	29	theme	in	544:545	arg1	ripening					552:559	in vivo ripening	544:559	in vivo ripening	544:559	The results suggested that the postharvest ripening process was dependent on the signal triggered by ABA and differed from in vivo ripening, resulting in fruits with altered chemical composition and firmness.
32078996	1	30	theme	Fragaria × ananassa	187:205	arg1	fruits					208:213	strawberry (Fragaria × ananassa) fruits	175:213	strawberry (Fragaria × ananassa) fruits that were ripened in vivo (attached to plant) to those ripened during postharvest storage	175:303	In this study, we compared the chemical composition of strawberry (Fragaria × ananassa) fruits that were ripened in vivo (attached to plant) to those ripened during postharvest storage.
32078996	6	31	theme	sucrose	1048:1054	arg1	role					1032:1035	the role	1028:1035	the role of ABA and sucrose in the regulation of postharvest ripening of strawberry	1028:1110	These results provide further insights into the role of ABA and sucrose in the regulation of postharvest ripening of strawberry.
32078996	4	32	theme	sucrose	649:655	arg1	application					634:644	The application	630:644	The application of sucrose in unripe strawberries	630:678	The application of sucrose in unripe strawberries resulted in the induction of ripening, which is dependent on ABA and its derivatives.
32078996	5	33	theme	strawberry	963:972	arg1	ripening					974:981	the postharvest strawberry ripening	947:981	the postharvest strawberry ripening	947:981	This induction was more pronounced during the first days of storage and associated with the application of mannitol rather than water, suggesting that mannitol negatively regulated the postharvest strawberry ripening.
32078996	1	34	dep	ripened	225:231	arg1	attached					242:249	attached	242:249	attached to plant	242:258	In this study, we compared the chemical composition of strawberry (Fragaria × ananassa) fruits that were ripened in vivo (attached to plant) to those ripened during postharvest storage.
32078996	4	35	theme	ripening	709:716	arg1	induction					696:704	the induction	692:704	the induction	692:704	The application of sucrose in unripe strawberries resulted in the induction of ripening, which is dependent on ABA and its derivatives.
32078996	4	35	theme	ripening	709:716	arg1	dependent					728:736	dependent	728:736	dependent	728:736	The application of sucrose in unripe strawberries resulted in the induction of ripening, which is dependent on ABA and its derivatives.
32078996	4	36	theme	unripe	660:665	arg1	strawberries					667:678	unripe strawberries	660:678	unripe strawberries	660:678	The application of sucrose in unripe strawberries resulted in the induction of ripening, which is dependent on ABA and its derivatives.
32078996	1	37	theme	fruits	208:213	arg1	composition					160:170	the chemical composition	147:170	the chemical composition of strawberry (Fragaria × ananassa) fruits that were ripened in vivo (attached to plant) to those ripened during postharvest storage	147:303	In this study, we compared the chemical composition of strawberry (Fragaria × ananassa) fruits that were ripened in vivo (attached to plant) to those ripened during postharvest storage.
32078996	6	38	theme	further	1006:1012	arg1	insights					1014:1021	further insights	1006:1021	further insights into the role of ABA and sucrose in the regulation of postharvest ripening of strawberry	1006:1110	These results provide further insights into the role of ABA and sucrose in the regulation of postharvest ripening of strawberry.
32078996	2	39	theme	application	325:335	arg1	effects					310:316	The effects	306:316	The effects of the application of abscisic acid (ABA) and sucrose on the postharvest ripening	306:398	The effects of the application of abscisic acid (ABA) and sucrose on the postharvest ripening were also evaluated.
32078996	0	40	theme	abscisic	57:64	arg1	acid					66:69	abscisic acid	57:69	abscisic acid	57:69	The postharvest ripening of strawberry fruits induced by abscisic acid and sucrose differs from their in vivo ripening.
32078996	1	41	theme	postharvest	285:295	arg1	storage					297:303	postharvest storage	285:303	postharvest storage	285:303	In this study, we compared the chemical composition of strawberry (Fragaria × ananassa) fruits that were ripened in vivo (attached to plant) to those ripened during postharvest storage.
34343588	0	0	theme	extract	89:95	arg1	microstructure					28:41	microstructure	28:41	microstructure	28:41	Physicochemical properties, microstructure, and storage stability of Pulicaria jaubertii extract microencapsulated with different protein biopolymers and gum arabic as wall materials.
34343588	0	0	theme	extract	89:95	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties, microstructure, and storage stability of Pulicaria jaubertii extract microencapsulated with different protein biopolymers and gum arabic as wall materials.
34343588	0	0	theme	extract	89:95	arg1	stability					56:64	storage stability	48:64	storage stability	48:64	Physicochemical properties, microstructure, and storage stability of Pulicaria jaubertii extract microencapsulated with different protein biopolymers and gum arabic as wall materials.
34343588	5	1	theme	content	1028:1034	arg1	compounds					1081:1089	particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds	895:1089	particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds	895:1089	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	5	2	theme	total	1013:1017	arg1	content					1028:1034	total phenolic content	1013:1034	total phenolic content (25.51 g GAE g-1 capsules)	1013:1061	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	5	2	theme	total	1013:1017	arg1	capsules					1053:1060	25.51 g GAE g-1 capsules	1037:1060	25.51 g GAE g-1 capsules	1037:1060	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	1	3	with	caseinate	328:336	arg1	materials					277:285	different protein materials	259:285	different protein materials	259:285	This study aimed to evaluate the possibility of using gum arabic (GA) with different protein materials namely whey protein isolate (WP), sodium caseinate (SC), and soybean protein (SP) as wall materials to encapsulate Pulicaria jaubertii extract (PJ) using freeze-drying.
34343588	0	4	theme	jaubertii	79:87	arg1	extract					89:95	Pulicaria jaubertii extract	69:95	Pulicaria jaubertii extract	69:95	Physicochemical properties, microstructure, and storage stability of Pulicaria jaubertii extract microencapsulated with different protein biopolymers and gum arabic as wall materials.
34343588	1	5	theme	sodium	321:326	arg1	SC					339:340	SC	339:340	SC	339:340	This study aimed to evaluate the possibility of using gum arabic (GA) with different protein materials namely whey protein isolate (WP), sodium caseinate (SC), and soybean protein (SP) as wall materials to encapsulate Pulicaria jaubertii extract (PJ) using freeze-drying.
34343588	1	5	theme	sodium	321:326	arg1	caseinate					328:336	sodium caseinate	321:336	sodium caseinate (SC)	321:341	This study aimed to evaluate the possibility of using gum arabic (GA) with different protein materials namely whey protein isolate (WP), sodium caseinate (SC), and soybean protein (SP) as wall materials to encapsulate Pulicaria jaubertii extract (PJ) using freeze-drying.
34343588	5	6	theme	high	1158:1161	arg1	stability					1173:1181	high oxidative stability	1158:1181	high oxidative stability	1158:1181	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	4	7	with	proteins	736:743	arg1	GA					750:751	GA	750:751	GA	750:751	The results indicated that mixtures of proteins with GA improved the physicochemical properties and bioactive content of the MPJE compared to GA-MPJE.
34343588	2	8	theme	microencapsulation	477:494	arg1	formulations					461:472	Four formulations	456:472	Four formulations of microencapsulation of Pulicaria jaubertii extract (MPJE)	456:532	Four formulations of microencapsulation of Pulicaria jaubertii extract (MPJE) were produced, including WPGA-MPJE, SCGA-MPJE, SPGA-MPJE, and GA-MPJE.
34343588	5	9	theme	25.51 g GAE g-1	1037:1051	arg1	content					1028:1034	total phenolic content	1013:1034	total phenolic content (25.51 g GAE g-1 capsules)	1013:1061	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	5	9	theme	25.51 g GAE g-1	1037:1051	arg1	capsules					1053:1060	25.51 g GAE g-1 capsules	1037:1060	25.51 g GAE g-1 capsules	1037:1060	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	5	10	theme	zeta	951:954	arg1	74.63 mV					967:974	74.63 mV	967:974	74.63 mV	967:974	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	5	10	theme	zeta	951:954	arg1	potential					956:964	zeta potential	951:964	zeta potential (74.63 mV)	951:975	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	5	11	theme	oxidative	1163:1171	arg1	stability					1173:1181	high oxidative stability	1158:1181	high oxidative stability	1158:1181	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	5	12	theme	presented	1103:1111	arg1	release					1121:1127	a lower release	1113:1127	presented a lower release of bioactive composites with high oxidative stability	1103:1181	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	4	13	theme	MPJE	822:825	arg1	properties					782:791	physicochemical properties	766:791	physicochemical properties	766:791	The results indicated that mixtures of proteins with GA improved the physicochemical properties and bioactive content of the MPJE compared to GA-MPJE.
34343588	4	13	theme	MPJE	822:825	arg1	content					807:813	bioactive content	797:813	bioactive content	797:813	The results indicated that mixtures of proteins with GA improved the physicochemical properties and bioactive content of the MPJE compared to GA-MPJE.
34343588	1	14	theme	different	259:267	arg1	materials					277:285	different protein materials	259:285	different protein materials	259:285	This study aimed to evaluate the possibility of using gum arabic (GA) with different protein materials namely whey protein isolate (WP), sodium caseinate (SC), and soybean protein (SP) as wall materials to encapsulate Pulicaria jaubertii extract (PJ) using freeze-drying.
34343588	0	15	theme	protein	130:136	arg1	biopolymers					138:148	different protein biopolymers	120:148	different protein biopolymers	120:148	Physicochemical properties, microstructure, and storage stability of Pulicaria jaubertii extract microencapsulated with different protein biopolymers and gum arabic as wall materials.
34343588	1	16	theme	Pulicaria	402:410	arg1	extract					422:428	Pulicaria jaubertii extract	402:428	Pulicaria jaubertii extract (PJ) using freeze-drying	402:453	This study aimed to evaluate the possibility of using gum arabic (GA) with different protein materials namely whey protein isolate (WP), sodium caseinate (SC), and soybean protein (SP) as wall materials to encapsulate Pulicaria jaubertii extract (PJ) using freeze-drying.
34343588	1	16	theme	Pulicaria	402:410	arg1	PJ					431:432	PJ	431:432	PJ	431:432	This study aimed to evaluate the possibility of using gum arabic (GA) with different protein materials namely whey protein isolate (WP), sodium caseinate (SC), and soybean protein (SP) as wall materials to encapsulate Pulicaria jaubertii extract (PJ) using freeze-drying.
34343588	5	17	with	composites	1142:1151	arg1	stability					1173:1181	high oxidative stability	1158:1181	high oxidative stability	1158:1181	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	0	18	theme	different	120:128	arg1	biopolymers					138:148	different protein biopolymers	120:148	different protein biopolymers	120:148	Physicochemical properties, microstructure, and storage stability of Pulicaria jaubertii extract microencapsulated with different protein biopolymers and gum arabic as wall materials.
34343588	7	19	theme	protein	1353:1359	arg1	mixtures					1361:1368	protein mixtures	1353:1368	protein mixtures with GA, especially the SCGA-MPJE formula,	1353:1411	The microcapsules prepared from protein mixtures with GA, especially the SCGA-MPJE formula, are the most efficient in encapsulating the plant extract derived from the PJ, which could be useful for application in various industrial fields.
34343588	7	20	from	application	1518:1528	arg1	fields					1552:1557	various industrial fields	1533:1557	various industrial fields	1533:1557	The microcapsules prepared from protein mixtures with GA, especially the SCGA-MPJE formula, are the most efficient in encapsulating the plant extract derived from the PJ, which could be useful for application in various industrial fields.
34343588	1	21	theme	jaubertii	412:420	arg1	extract					422:428	Pulicaria jaubertii extract	402:428	Pulicaria jaubertii extract (PJ) using freeze-drying	402:453	This study aimed to evaluate the possibility of using gum arabic (GA) with different protein materials namely whey protein isolate (WP), sodium caseinate (SC), and soybean protein (SP) as wall materials to encapsulate Pulicaria jaubertii extract (PJ) using freeze-drying.
34343588	1	21	theme	jaubertii	412:420	arg1	PJ					431:432	PJ	431:432	PJ	431:432	This study aimed to evaluate the possibility of using gum arabic (GA) with different protein materials namely whey protein isolate (WP), sodium caseinate (SC), and soybean protein (SP) as wall materials to encapsulate Pulicaria jaubertii extract (PJ) using freeze-drying.
34343588	3	22	theme	storage	678:684	arg1	stability					686:694	the storage stability	674:694	the storage stability	674:694	The formulations were stored at 4 °C and 25 °C for 28 days to assess the storage stability.
34343588	5	23	theme	antioxidants	1068:1079	arg1	compounds					1081:1089	particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds	895:1089	particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds	895:1089	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	0	24	theme	Physicochemical	0:14	arg1	properties					16:25	Physicochemical properties	0:25	Physicochemical properties	0:25	Physicochemical properties, microstructure, and storage stability of Pulicaria jaubertii extract microencapsulated with different protein biopolymers and gum arabic as wall materials.
34343588	1	25	theme	protein	269:275	arg1	materials					277:285	different protein materials	259:285	different protein materials	259:285	This study aimed to evaluate the possibility of using gum arabic (GA) with different protein materials namely whey protein isolate (WP), sodium caseinate (SC), and soybean protein (SP) as wall materials to encapsulate Pulicaria jaubertii extract (PJ) using freeze-drying.
34343588	4	26	theme	bioactive	797:805	arg1	content					807:813	bioactive content	797:813	bioactive content	797:813	The results indicated that mixtures of proteins with GA improved the physicochemical properties and bioactive content of the MPJE compared to GA-MPJE.
34343588	7	27	theme	SCGA-MPJE	1394:1402	arg1	formula					1404:1410	the SCGA-MPJE formula	1390:1410	especially the SCGA-MPJE formula	1379:1410	The microcapsules prepared from protein mixtures with GA, especially the SCGA-MPJE formula, are the most efficient in encapsulating the plant extract derived from the PJ, which could be useful for application in various industrial fields.
34343588	7	27	theme	SCGA-MPJE	1394:1402	arg1	GA					1375:1376	GA	1375:1376	GA	1375:1376	The microcapsules prepared from protein mixtures with GA, especially the SCGA-MPJE formula, are the most efficient in encapsulating the plant extract derived from the PJ, which could be useful for application in various industrial fields.
34343588	1	28	with	protein	356:362	arg1	materials					277:285	different protein materials	259:285	different protein materials	259:285	This study aimed to evaluate the possibility of using gum arabic (GA) with different protein materials namely whey protein isolate (WP), sodium caseinate (SC), and soybean protein (SP) as wall materials to encapsulate Pulicaria jaubertii extract (PJ) using freeze-drying.
34343588	5	29	theme	efficiency	992:1001	arg1	compounds					1081:1089	particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds	895:1089	particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds	895:1089	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	5	30	theme	compounds	1081:1089	arg1	release					1121:1127	a lower release	1113:1127	presented a lower release of bioactive composites with high oxidative stability	1103:1181	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	5	30	theme	compounds	1081:1089	arg1	values					885:890	optimal values	877:890	optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds	877:1089	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	2	31	theme	extract	519:525	arg1	microencapsulation					477:494	microencapsulation	477:494	microencapsulation of Pulicaria jaubertii extract (MPJE)	477:532	Four formulations of microencapsulation of Pulicaria jaubertii extract (MPJE) were produced, including WPGA-MPJE, SCGA-MPJE, SPGA-MPJE, and GA-MPJE.
34343588	5	32	theme	particle	895:902	arg1	450.13 nm					910:918	450.13 nm	910:918	450.13 nm	910:918	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	5	32	theme	particle	895:902	arg1	size					904:907	particle size	895:907	particle size (450.13 nm)	895:919	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	4	33	dep	properties	782:791	arg1	the					762:764	the	762:764	the	762:764	The results indicated that mixtures of proteins with GA improved the physicochemical properties and bioactive content of the MPJE compared to GA-MPJE.
34343588	2	34	theme	jaubertii	509:517	arg1	MPJE					528:531	MPJE	528:531	MPJE	528:531	Four formulations of microencapsulation of Pulicaria jaubertii extract (MPJE) were produced, including WPGA-MPJE, SCGA-MPJE, SPGA-MPJE, and GA-MPJE.
34343588	2	34	theme	jaubertii	509:517	arg1	extract					519:525	Pulicaria jaubertii extract	499:525	Pulicaria jaubertii extract (MPJE)	499:532	Four formulations of microencapsulation of Pulicaria jaubertii extract (MPJE) were produced, including WPGA-MPJE, SCGA-MPJE, SPGA-MPJE, and GA-MPJE.
34343588	1	35	dep	arabic	242:247	arg1	isolate					307:313	whey protein isolate	294:313	whey protein isolate (WP)	294:318	This study aimed to evaluate the possibility of using gum arabic (GA) with different protein materials namely whey protein isolate (WP), sodium caseinate (SC), and soybean protein (SP) as wall materials to encapsulate Pulicaria jaubertii extract (PJ) using freeze-drying.
34343588	1	35	dep	arabic	242:247	arg1	WP					316:317	WP	316:317	WP	316:317	This study aimed to evaluate the possibility of using gum arabic (GA) with different protein materials namely whey protein isolate (WP), sodium caseinate (SC), and soybean protein (SP) as wall materials to encapsulate Pulicaria jaubertii extract (PJ) using freeze-drying.
34343588	5	36	theme	size	904:907	arg1	compounds					1081:1089	particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds	895:1089	particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds	895:1089	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	7	37	theme	industrial	1541:1550	arg1	fields					1552:1557	various industrial fields	1533:1557	various industrial fields	1533:1557	The microcapsules prepared from protein mixtures with GA, especially the SCGA-MPJE formula, are the most efficient in encapsulating the plant extract derived from the PJ, which could be useful for application in various industrial fields.
34343588	1	38	theme	whey	294:297	arg1	isolate					307:313	whey protein isolate	294:313	whey protein isolate (WP)	294:318	This study aimed to evaluate the possibility of using gum arabic (GA) with different protein materials namely whey protein isolate (WP), sodium caseinate (SC), and soybean protein (SP) as wall materials to encapsulate Pulicaria jaubertii extract (PJ) using freeze-drying.
34343588	1	38	theme	whey	294:297	arg1	WP					316:317	WP	316:317	WP	316:317	This study aimed to evaluate the possibility of using gum arabic (GA) with different protein materials namely whey protein isolate (WP), sodium caseinate (SC), and soybean protein (SP) as wall materials to encapsulate Pulicaria jaubertii extract (PJ) using freeze-drying.
34343588	2	39	theme	Pulicaria	499:507	arg1	MPJE					528:531	MPJE	528:531	MPJE	528:531	Four formulations of microencapsulation of Pulicaria jaubertii extract (MPJE) were produced, including WPGA-MPJE, SCGA-MPJE, SPGA-MPJE, and GA-MPJE.
34343588	2	39	theme	Pulicaria	499:507	arg1	extract					519:525	Pulicaria jaubertii extract	499:525	Pulicaria jaubertii extract (MPJE)	499:532	Four formulations of microencapsulation of Pulicaria jaubertii extract (MPJE) were produced, including WPGA-MPJE, SCGA-MPJE, SPGA-MPJE, and GA-MPJE.
34343588	1	40	theme	soybean	348:354	arg1	protein					356:362	soybean protein	348:362	soybean protein (SP)	348:367	This study aimed to evaluate the possibility of using gum arabic (GA) with different protein materials namely whey protein isolate (WP), sodium caseinate (SC), and soybean protein (SP) as wall materials to encapsulate Pulicaria jaubertii extract (PJ) using freeze-drying.
34343588	1	40	theme	soybean	348:354	arg1	SP					365:366	SP	365:366	SP	365:366	This study aimed to evaluate the possibility of using gum arabic (GA) with different protein materials namely whey protein isolate (WP), sodium caseinate (SC), and soybean protein (SP) as wall materials to encapsulate Pulicaria jaubertii extract (PJ) using freeze-drying.
34343588	7	41	attach	derived	1471:1477	arg1	useful					1507:1512	useful	1507:1512	useful	1507:1512	The microcapsules prepared from protein mixtures with GA, especially the SCGA-MPJE formula, are the most efficient in encapsulating the plant extract derived from the PJ, which could be useful for application in various industrial fields.
34343588	7	41	attach	derived	1471:1477	arg1	PJ					1488:1489	the PJ	1484:1489	the PJ	1484:1489	The microcapsules prepared from protein mixtures with GA, especially the SCGA-MPJE formula, are the most efficient in encapsulating the plant extract derived from the PJ, which could be useful for application in various industrial fields.
34343588	7	41	attach	derived	1471:1477	arg2	extract					1463:1469	the plant extract	1453:1469	the plant extract derived from the PJ, which could be useful for application in various industrial fields	1453:1557	The microcapsules prepared from protein mixtures with GA, especially the SCGA-MPJE formula, are the most efficient in encapsulating the plant extract derived from the PJ, which could be useful for application in various industrial fields.
34343588	1	42	theme	protein	299:305	arg1	isolate					307:313	whey protein isolate	294:313	whey protein isolate (WP)	294:318	This study aimed to evaluate the possibility of using gum arabic (GA) with different protein materials namely whey protein isolate (WP), sodium caseinate (SC), and soybean protein (SP) as wall materials to encapsulate Pulicaria jaubertii extract (PJ) using freeze-drying.
34343588	1	42	theme	protein	299:305	arg1	WP					316:317	WP	316:317	WP	316:317	This study aimed to evaluate the possibility of using gum arabic (GA) with different protein materials namely whey protein isolate (WP), sodium caseinate (SC), and soybean protein (SP) as wall materials to encapsulate Pulicaria jaubertii extract (PJ) using freeze-drying.
34343588	6	43	theme	formulations	1244:1255	arg1	microstructure					1221:1234	The microstructure	1217:1234	The microstructure of MPJE formulations	1217:1255	The microstructure of MPJE formulations showed a flat surface without any visible cracking on surfaces.
34343588	5	44	theme	encapsulation	978:990	arg1	efficiency					992:1001	encapsulation efficiency	978:1001	encapsulation efficiency (91.07%)	978:1010	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	5	44	theme	encapsulation	978:990	arg1	%					1009:1009	91.07%	1004:1009	91.07%	1004:1009	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	6	45	theme	flat	1266:1269	arg1	surface					1271:1277	a flat surface	1264:1277	a flat surface	1264:1277	The microstructure of MPJE formulations showed a flat surface without any visible cracking on surfaces.
34343588	0	46	theme	wall	168:171	arg1	materials					173:181	wall materials	168:181	wall materials	168:181	Physicochemical properties, microstructure, and storage stability of Pulicaria jaubertii extract microencapsulated with different protein biopolymers and gum arabic as wall materials.
34343588	7	47	theme	various	1533:1539	arg1	fields					1552:1557	various industrial fields	1533:1557	various industrial fields	1533:1557	The microcapsules prepared from protein mixtures with GA, especially the SCGA-MPJE formula, are the most efficient in encapsulating the plant extract derived from the PJ, which could be useful for application in various industrial fields.
34343588	4	48	theme	physicochemical	766:780	arg1	properties					782:791	physicochemical properties	766:791	physicochemical properties	766:791	The results indicated that mixtures of proteins with GA improved the physicochemical properties and bioactive content of the MPJE compared to GA-MPJE.
34343588	5	49	theme	SCGA-MPJE	852:860	arg1	formula					862:868	The SCGA-MPJE formula	848:868	The SCGA-MPJE formula	848:868	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	0	50	theme	storage	48:54	arg1	stability					56:64	storage stability	48:64	storage stability	48:64	Physicochemical properties, microstructure, and storage stability of Pulicaria jaubertii extract microencapsulated with different protein biopolymers and gum arabic as wall materials.
34343588	6	51	theme	MPJE	1239:1242	arg1	formulations					1244:1255	MPJE formulations	1239:1255	MPJE formulations	1239:1255	The microstructure of MPJE formulations showed a flat surface without any visible cracking on surfaces.
34343588	5	52	theme	potential	956:964	arg1	compounds					1081:1089	particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds	895:1089	particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds	895:1089	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	3	53	dep	assess	667:672	arg1	28 days					656:662	28 days	656:662	28 days	656:662	The formulations were stored at 4 °C and 25 °C for 28 days to assess the storage stability.
34343588	5	54	theme	polydispersity	922:935	arg1	0.33					944:947	0.33	944:947	0.33	944:947	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	5	54	theme	polydispersity	922:935	arg1	index					937:941	polydispersity index	922:941	polydispersity index (0.33)	922:948	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	7	55	with	mixtures	1361:1368	arg1	formula					1404:1410	the SCGA-MPJE formula	1390:1410	especially the SCGA-MPJE formula	1379:1410	The microcapsules prepared from protein mixtures with GA, especially the SCGA-MPJE formula, are the most efficient in encapsulating the plant extract derived from the PJ, which could be useful for application in various industrial fields.
34343588	7	55	with	mixtures	1361:1368	arg1	GA					1375:1376	GA	1375:1376	GA	1375:1376	The microcapsules prepared from protein mixtures with GA, especially the SCGA-MPJE formula, are the most efficient in encapsulating the plant extract derived from the PJ, which could be useful for application in various industrial fields.
34343588	5	56	theme	lower	1115:1119	arg1	release					1121:1127	a lower release	1113:1127	presented a lower release of bioactive composites with high oxidative stability	1103:1181	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	5	57	theme	index	937:941	arg1	compounds					1081:1089	particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds	895:1089	particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds	895:1089	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	1	58	with	arabic	242:247	arg1	materials					277:285	different protein materials	259:285	different protein materials	259:285	This study aimed to evaluate the possibility of using gum arabic (GA) with different protein materials namely whey protein isolate (WP), sodium caseinate (SC), and soybean protein (SP) as wall materials to encapsulate Pulicaria jaubertii extract (PJ) using freeze-drying.
34343588	5	59	theme	optimal	877:883	arg1	values					885:890	optimal values	877:890	optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds	877:1089	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	1	60	theme	gum	238:240	arg1	arabic					242:247	gum arabic	238:247	gum arabic (GA) with different protein materials	238:285	This study aimed to evaluate the possibility of using gum arabic (GA) with different protein materials namely whey protein isolate (WP), sodium caseinate (SC), and soybean protein (SP) as wall materials to encapsulate Pulicaria jaubertii extract (PJ) using freeze-drying.
34343588	1	60	theme	gum	238:240	arg1	GA					250:251	GA	250:251	GA	250:251	This study aimed to evaluate the possibility of using gum arabic (GA) with different protein materials namely whey protein isolate (WP), sodium caseinate (SC), and soybean protein (SP) as wall materials to encapsulate Pulicaria jaubertii extract (PJ) using freeze-drying.
34343588	0	61	theme	Pulicaria	69:77	arg1	extract					89:95	Pulicaria jaubertii extract	69:95	Pulicaria jaubertii extract	69:95	Physicochemical properties, microstructure, and storage stability of Pulicaria jaubertii extract microencapsulated with different protein biopolymers and gum arabic as wall materials.
34343588	1	62	theme	wall	372:375	arg1	materials					377:385	wall materials	372:385	wall materials	372:385	This study aimed to evaluate the possibility of using gum arabic (GA) with different protein materials namely whey protein isolate (WP), sodium caseinate (SC), and soybean protein (SP) as wall materials to encapsulate Pulicaria jaubertii extract (PJ) using freeze-drying.
34343588	7	63	theme	plant	1457:1461	arg1	extract					1463:1469	the plant extract	1453:1469	the plant extract derived from the PJ, which could be useful for application in various industrial fields	1453:1557	The microcapsules prepared from protein mixtures with GA, especially the SCGA-MPJE formula, are the most efficient in encapsulating the plant extract derived from the PJ, which could be useful for application in various industrial fields.
34343588	5	64	theme	phenolic	1019:1026	arg1	content					1028:1034	total phenolic content	1013:1034	total phenolic content (25.51 g GAE g-1 capsules)	1013:1061	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	5	64	theme	phenolic	1019:1026	arg1	capsules					1053:1060	25.51 g GAE g-1 capsules	1037:1060	25.51 g GAE g-1 capsules	1037:1060	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	4	65	theme	proteins	736:743	arg1	mixtures					724:731	mixtures	724:731	mixtures of proteins with GA	724:751	The results indicated that mixtures of proteins with GA improved the physicochemical properties and bioactive content of the MPJE compared to GA-MPJE.
34343588	5	66	theme	composites	1142:1151	arg1	release					1121:1127	a lower release	1113:1127	presented a lower release of bioactive composites with high oxidative stability	1103:1181	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	5	66	theme	composites	1142:1151	arg1	values					885:890	optimal values	877:890	optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds	877:1089	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
34343588	5	67	theme	bioactive	1132:1140	arg1	composites					1142:1151	bioactive composites	1132:1151	bioactive composites with high oxidative stability	1132:1181	The SCGA-MPJE formula showed optimal values of particle size (450.13 nm), polydispersity index (0.33), zeta potential (74.63 mV), encapsulation efficiency (91.07%), total phenolic content (25.51 g GAE g-1 capsules), and antioxidants compounds, as well as presented a lower release of bioactive composites with high oxidative stability during storage at 4 °C and 25 °C.
32008782	8	0	mod	modified	1255:1262	arg3	parity					1267:1272	parity	1267:1272	parity	1267:1272	The effect of treatment on MUOT was modified by parity.
32008782	8	0	mod	modified	1255:1262	arg1	effect					1223:1228	The effect	1219:1228	The effect of treatment on MUOT	1219:1249	The effect of treatment on MUOT was modified by parity.
32008782	10	1	theme	component	1581:1589	arg1	yields					1591:1596	milk component yields	1576:1596	milk component yields	1576:1596	We detected no meaningful differences in milk component yields or linear somatic cell score.
32008782	3	2	theme	flow	556:559	arg1	threshold					561:569	a cluster remover take-off milk flow threshold	524:569	a cluster remover take-off milk flow threshold of 1.2 (ACR1.2) or 0.8 kg/min (ACR0.8) for 57 d. Milking characteristics (milk yield; and milking unit-on time, MUOT) were obtained with electronic on-farm milk meters	524:737	Treatment consisted of a cluster remover take-off milk flow threshold of 1.2 (ACR1.2) or 0.8 kg/min (ACR0.8) for 57 d. Milking characteristics (milk yield; and milking unit-on time, MUOT) were obtained with electronic on-farm milk meters.
32008782	13	3	theme	short-term	2066:2075	arg1	changes					2077:2083	short-term changes	2066:2083	short-term changes to the teat tissue	2066:2102	The odds of short-term changes to the teat tissue were lower for cows in group ACR1.2 [odds ratio (95% confidence interval) = 0.78 (0.63-0.96)].
32008782	15	4	theme	machine-milking-induced	2442:2464	arg1	changes					2477:2483	machine-milking-induced short-term changes	2442:2483	machine-milking-induced short-term changes to the teat tissue	2442:2502	Increasing cluster remover take-off milk flow threshold from 0.8 to 1.2 kg/min decreased individual milking duration and alleviated machine-milking-induced short-term changes to the teat tissue without adversely affecting milking performance or somatic cell count.
32008782	11	5	theme	somatic	1889:1895	arg1	score					1902:1906	linear somatic cell score	1882:1906	linear somatic cell score	1882:1906	Least squares means in groups ACR1.2 and ACR0.8, respectively, were milk fat yield, 0.42 (0.40-0.44) and 0.42 (0.40-0.44) kg; milk protein yield, 0.36 (0.35-0.37) and 0.37 (0.36-0.37) kg; milk lactose yield, 0.61 (0.60-0.63) and 0.63 (0.61-0.64) kg, and linear somatic cell score, 1.9 (1.8-2.0) and 1.9 (1.8-2.0).
32008782	10	6	theme	cell	1616:1619	arg1	score					1621:1625	linear somatic cell score	1601:1625	linear somatic cell score	1601:1625	We detected no meaningful differences in milk component yields or linear somatic cell score.
32008782	11	7	theme	protein	1759:1765	arg1	kg					1812:1813	kg	1812:1813	kg	1812:1813	Least squares means in groups ACR1.2 and ACR0.8, respectively, were milk fat yield, 0.42 (0.40-0.44) and 0.42 (0.40-0.44) kg; milk protein yield, 0.36 (0.35-0.37) and 0.37 (0.36-0.37) kg; milk lactose yield, 0.61 (0.60-0.63) and 0.63 (0.61-0.64) kg, and linear somatic cell score, 1.9 (1.8-2.0) and 1.9 (1.8-2.0).
32008782	11	7	theme	protein	1759:1765	arg1	0.35-0.37					1780:1788	0.35-0.37	1780:1788	0.35-0.37	1780:1788	Least squares means in groups ACR1.2 and ACR0.8, respectively, were milk fat yield, 0.42 (0.40-0.44) and 0.42 (0.40-0.44) kg; milk protein yield, 0.36 (0.35-0.37) and 0.37 (0.36-0.37) kg; milk lactose yield, 0.61 (0.60-0.63) and 0.63 (0.61-0.64) kg, and linear somatic cell score, 1.9 (1.8-2.0) and 1.9 (1.8-2.0).
32008782	11	7	theme	protein	1759:1765	arg1	yield					1767:1771	milk protein yield	1754:1771	milk protein yield	1754:1771	Least squares means in groups ACR1.2 and ACR0.8, respectively, were milk fat yield, 0.42 (0.40-0.44) and 0.42 (0.40-0.44) kg; milk protein yield, 0.36 (0.35-0.37) and 0.37 (0.36-0.37) kg; milk lactose yield, 0.61 (0.60-0.63) and 0.63 (0.61-0.64) kg, and linear somatic cell score, 1.9 (1.8-2.0) and 1.9 (1.8-2.0).
32008782	15	8	theme	milking	2410:2416	arg1	duration					2418:2425	individual milking duration	2399:2425	individual milking duration	2399:2425	Increasing cluster remover take-off milk flow threshold from 0.8 to 1.2 kg/min decreased individual milking duration and alleviated machine-milking-induced short-term changes to the teat tissue without adversely affecting milking performance or somatic cell count.
32008782	12	9	theme	treatment	1997:2005	arg1	effect					1987:1992	an effect	1984:1992	an effect of treatment on machine-milking-induced short-term changes	1984:2051	A generalized linear mixed model revealed an effect of treatment on machine-milking-induced short-term changes.
32008782	10	10	theme	linear	1601:1606	arg1	score					1621:1625	linear somatic cell score	1601:1625	linear somatic cell score	1601:1625	We detected no meaningful differences in milk component yields or linear somatic cell score.
32008782	2	11	theme	treatment	483:491	arg1	groups					493:498	2 treatment groups	481:498	2 treatment groups	481:498	In a randomized controlled field trial, Holstein cows (n = 689) from 1 commercial dairy farm with a thrice-daily milking schedule were allocated to 2 treatment groups.
32008782	12	12	theme	machine-milking-induced	2010:2032	arg1	changes					2045:2051	machine-milking-induced short-term changes	2010:2051	machine-milking-induced short-term changes	2010:2051	A generalized linear mixed model revealed an effect of treatment on machine-milking-induced short-term changes.
32008782	10	13	theme	milk	1576:1579	arg1	yields					1591:1596	milk component yields	1576:1596	milk component yields	1576:1596	We detected no meaningful differences in milk component yields or linear somatic cell score.
32008782	16	14	from	effect	2623:2628	arg1	production					2638:2647	milk production	2633:2647	milk production	2633:2647	Future studies are warranted to investigate the effect on milk production and udder health over a whole lactation period.
32008782	16	14	from	effect	2623:2628	arg1	health					2659:2664	udder health	2653:2664	udder health	2653:2664	Future studies are warranted to investigate the effect on milk production and udder health over a whole lactation period.
32008782	5	15	theme	Machine-milking-induced	842:864	arg1	changes					887:893	Machine-milking-induced short- and long-term changes	842:893	Machine-milking-induced short- and long-term changes to the teat tissue condition	842:922	Machine-milking-induced short- and long-term changes to the teat tissue condition were assessed visually.
32008782	13	16	theme	interval	2168:2175	arg1	%					2155:2155	95%	2153:2155	95% confidence interval	2153:2175	The odds of short-term changes to the teat tissue were lower for cows in group ACR1.2 [odds ratio (95% confidence interval) = 0.78 (0.63-0.96)].
32008782	15	17	theme	cluster	2321:2327	arg1	threshold					2356:2364	cluster remover take-off milk flow threshold	2321:2364	cluster remover take-off milk flow threshold	2321:2364	Increasing cluster remover take-off milk flow threshold from 0.8 to 1.2 kg/min decreased individual milking duration and alleviated machine-milking-induced short-term changes to the teat tissue without adversely affecting milking performance or somatic cell count.
32008782	4	18	theme	Composite	740:748	arg1	samples					755:761	Composite milk samples	740:761	Composite milk samples	740:761	Composite milk samples were collected and analyzed for fat, protein, lactose, and somatic cell count.
32008782	15	19	theme	teat	2492:2495	arg1	tissue					2497:2502	the teat tissue	2488:2502	the teat tissue	2488:2502	Increasing cluster remover take-off milk flow threshold from 0.8 to 1.2 kg/min decreased individual milking duration and alleviated machine-milking-induced short-term changes to the teat tissue without adversely affecting milking performance or somatic cell count.
32008782	3	20	theme	0.8	590:592	arg1	threshold					561:569	a cluster remover take-off milk flow threshold	524:569	a cluster remover take-off milk flow threshold of 1.2 (ACR1.2) or 0.8 kg/min (ACR0.8) for 57 d. Milking characteristics (milk yield; and milking unit-on time, MUOT) were obtained with electronic on-farm milk meters	524:737	Treatment consisted of a cluster remover take-off milk flow threshold of 1.2 (ACR1.2) or 0.8 kg/min (ACR0.8) for 57 d. Milking characteristics (milk yield; and milking unit-on time, MUOT) were obtained with electronic on-farm milk meters.
32008782	0	21	theme	teat	101:104	arg1	condition					106:114	teat condition	101:114	teat condition	101:114	A randomized trial to study the effect of automatic cluster remover settings on milking performance, teat condition, and udder health.
32008782	3	22	dep	Milking	620:626	arg1	obtained					694:701	obtained	694:701	were obtained with electronic on-farm milk meters	689:737	Treatment consisted of a cluster remover take-off milk flow threshold of 1.2 (ACR1.2) or 0.8 kg/min (ACR0.8) for 57 d. Milking characteristics (milk yield; and milking unit-on time, MUOT) were obtained with electronic on-farm milk meters.
32008782	2	23	theme	commercial	404:413	arg1	farm					421:424	1 commercial dairy farm	402:424	1 commercial dairy farm with a thrice-daily milking schedule	402:461	In a randomized controlled field trial, Holstein cows (n = 689) from 1 commercial dairy farm with a thrice-daily milking schedule were allocated to 2 treatment groups.
32008782	11	24	theme	squares	1634:1640	arg1	means					1642:1646	Least squares means	1628:1646	Least squares means in groups ACR1.2 and ACR0.8	1628:1674	Least squares means in groups ACR1.2 and ACR0.8, respectively, were milk fat yield, 0.42 (0.40-0.44) and 0.42 (0.40-0.44) kg; milk protein yield, 0.36 (0.35-0.37) and 0.37 (0.36-0.37) kg; milk lactose yield, 0.61 (0.60-0.63) and 0.63 (0.61-0.64) kg, and linear somatic cell score, 1.9 (1.8-2.0) and 1.9 (1.8-2.0).
32008782	2	25	theme	milking	446:452	arg1	schedule					454:461	a thrice-daily milking schedule	431:461	a thrice-daily milking schedule	431:461	In a randomized controlled field trial, Holstein cows (n = 689) from 1 commercial dairy farm with a thrice-daily milking schedule were allocated to 2 treatment groups.
32008782	0	26	theme	udder	121:125	arg1	health					127:132	udder health	121:132	udder health	121:132	A randomized trial to study the effect of automatic cluster remover settings on milking performance, teat condition, and udder health.
32008782	3	27	dep	yield	650:654	arg1	MUOT					683:686	MUOT	683:686	MUOT	683:686	Treatment consisted of a cluster remover take-off milk flow threshold of 1.2 (ACR1.2) or 0.8 kg/min (ACR0.8) for 57 d. Milking characteristics (milk yield; and milking unit-on time, MUOT) were obtained with electronic on-farm milk meters.
32008782	13	28	dep	group	2127:2131	arg1	=					2178:2178	=	2178:2178	=	2178:2178	The odds of short-term changes to the teat tissue were lower for cows in group ACR1.2 [odds ratio (95% confidence interval) = 0.78 (0.63-0.96)].
32008782	14	29	theme	treatment	2292:2300	arg1	groups					2302:2307	treatment groups	2292:2307	treatment groups	2292:2307	No meaningful differences were detected in machine-milking-induced long-term changes between treatment groups.
32008782	15	30	theme	take-off	2337:2344	arg1	threshold					2356:2364	cluster remover take-off milk flow threshold	2321:2364	cluster remover take-off milk flow threshold	2321:2364	Increasing cluster remover take-off milk flow threshold from 0.8 to 1.2 kg/min decreased individual milking duration and alleviated machine-milking-induced short-term changes to the teat tissue without adversely affecting milking performance or somatic cell count.
32008782	1	31	theme	udder	319:323	arg1	health					325:330	(4) udder health	315:330	(4) udder health	315:330	The objectives were to study the effect of 2 different automatic cluster remover settings on (1) milking characteristics, (2) milk component yields, (3) teat tissue condition, and (4) udder health.
32008782	9	32	theme	Milking	1275:1281	arg1	time					1291:1294	Milking unit-on time	1275:1294	Milking unit-on time	1275:1294	Milking unit-on time in first-, second-, and ≥third-lactation cows, respectively, was 260.7 (252.0-269.4), 257.8 (247.4-268.1), and 260.2 (252.6-267.9) s in group ACR1.2; and 273.7 (264.9-282.5), 279.1 (269.4-288.8), and 295.7 (287.9-303.6) s in group ACR0.8.
32008782	3	33	theme	unit-on	669:675	arg1	time					677:680	milking unit-on time	661:680	milking unit-on time	661:680	Treatment consisted of a cluster remover take-off milk flow threshold of 1.2 (ACR1.2) or 0.8 kg/min (ACR0.8) for 57 d. Milking characteristics (milk yield; and milking unit-on time, MUOT) were obtained with electronic on-farm milk meters.
32008782	3	33	theme	unit-on	669:675	arg1	characteristics					628:642	characteristics	628:642	characteristics (milk yield; and milking unit-on time, MUOT)	628:687	Treatment consisted of a cluster remover take-off milk flow threshold of 1.2 (ACR1.2) or 0.8 kg/min (ACR0.8) for 57 d. Milking characteristics (milk yield; and milking unit-on time, MUOT) were obtained with electronic on-farm milk meters.
32008782	6	34	theme	meaningful	1021:1030	arg1	differences					1032:1042	no meaningful differences	1018:1042	no meaningful differences in milk yield	1018:1056	General linear mixed models demonstrated differences in MUOT, whereas no meaningful differences in milk yield were detected.
32008782	0	35	theme	automatic	42:50	arg1	settings					68:75	automatic cluster remover settings	42:75	automatic cluster remover settings	42:75	A randomized trial to study the effect of automatic cluster remover settings on milking performance, teat condition, and udder health.
32008782	1	36	theme	component	266:274	arg1	yields					276:281	(2) milk component yields	257:281	(2) milk component yields	257:281	The objectives were to study the effect of 2 different automatic cluster remover settings on (1) milking characteristics, (2) milk component yields, (3) teat tissue condition, and (4) udder health.
32008782	0	37	theme	remover	60:66	arg1	settings					68:75	automatic cluster remover settings	42:75	automatic cluster remover settings	42:75	A randomized trial to study the effect of automatic cluster remover settings on milking performance, teat condition, and udder health.
32008782	6	38	from	differences	1032:1042	arg1	yield					1052:1056	milk yield	1047:1056	milk yield	1047:1056	General linear mixed models demonstrated differences in MUOT, whereas no meaningful differences in milk yield were detected.
32008782	5	39	theme	tissue	907:912	arg1	condition					914:922	the teat tissue condition	898:922	the teat tissue condition	898:922	Machine-milking-induced short- and long-term changes to the teat tissue condition were assessed visually.
32008782	3	40	theme	milk	727:730	arg1	meters					732:737	electronic on-farm milk meters	708:737	electronic on-farm milk meters	708:737	Treatment consisted of a cluster remover take-off milk flow threshold of 1.2 (ACR1.2) or 0.8 kg/min (ACR0.8) for 57 d. Milking characteristics (milk yield; and milking unit-on time, MUOT) were obtained with electronic on-farm milk meters.
32008782	9	41	from	s	1427:1427	arg1	cows					1337:1340	≥third-lactation cows	1320:1340	≥third-lactation cows	1320:1340	Milking unit-on time in first-, second-, and ≥third-lactation cows, respectively, was 260.7 (252.0-269.4), 257.8 (247.4-268.1), and 260.2 (252.6-267.9) s in group ACR1.2; and 273.7 (264.9-282.5), 279.1 (269.4-288.8), and 295.7 (287.9-303.6) s in group ACR0.8.
32008782	9	41	from	s	1427:1427	arg1	group					1521:1525	group ACR0.8	1521:1532	group ACR0.8	1521:1532	Milking unit-on time in first-, second-, and ≥third-lactation cows, respectively, was 260.7 (252.0-269.4), 257.8 (247.4-268.1), and 260.2 (252.6-267.9) s in group ACR1.2; and 273.7 (264.9-282.5), 279.1 (269.4-288.8), and 295.7 (287.9-303.6) s in group ACR0.8.
32008782	9	41	from	s	1427:1427	arg1	group					1432:1436	group ACR1.2	1432:1443	group ACR1.2	1432:1443	Milking unit-on time in first-, second-, and ≥third-lactation cows, respectively, was 260.7 (252.0-269.4), 257.8 (247.4-268.1), and 260.2 (252.6-267.9) s in group ACR1.2; and 273.7 (264.9-282.5), 279.1 (269.4-288.8), and 295.7 (287.9-303.6) s in group ACR0.8.
32008782	9	41	from	s	1427:1427	arg1	second-					1307:1313	second-	1307:1313	second-	1307:1313	Milking unit-on time in first-, second-, and ≥third-lactation cows, respectively, was 260.7 (252.0-269.4), 257.8 (247.4-268.1), and 260.2 (252.6-267.9) s in group ACR1.2; and 273.7 (264.9-282.5), 279.1 (269.4-288.8), and 295.7 (287.9-303.6) s in group ACR0.8.
32008782	9	41	from	s	1427:1427	arg1	first-					1299:1304	first-	1299:1304	first-	1299:1304	Milking unit-on time in first-, second-, and ≥third-lactation cows, respectively, was 260.7 (252.0-269.4), 257.8 (247.4-268.1), and 260.2 (252.6-267.9) s in group ACR1.2; and 273.7 (264.9-282.5), 279.1 (269.4-288.8), and 295.7 (287.9-303.6) s in group ACR0.8.
32008782	9	42	theme	269.4-288.8	1478:1488	arg1	s					1516:1516	273.7 (264.9-282.5), 279.1 (269.4-288.8), and 295.7 (287.9-303.6) s	1450:1516	273.7 (264.9-282.5), 279.1 (269.4-288.8), and 295.7 (287.9-303.6) s in group ACR0.8	1450:1532	Milking unit-on time in first-, second-, and ≥third-lactation cows, respectively, was 260.7 (252.0-269.4), 257.8 (247.4-268.1), and 260.2 (252.6-267.9) s in group ACR1.2; and 273.7 (264.9-282.5), 279.1 (269.4-288.8), and 295.7 (287.9-303.6) s in group ACR0.8.
32008782	0	43	from	effect	32:37	arg1	health					127:132	udder health	121:132	udder health	121:132	A randomized trial to study the effect of automatic cluster remover settings on milking performance, teat condition, and udder health.
32008782	0	43	from	effect	32:37	arg1	performance					88:98	milking performance	80:98	milking performance	80:98	A randomized trial to study the effect of automatic cluster remover settings on milking performance, teat condition, and udder health.
32008782	0	43	from	effect	32:37	arg1	condition					106:114	teat condition	101:114	teat condition	101:114	A randomized trial to study the effect of automatic cluster remover settings on milking performance, teat condition, and udder health.
32008782	14	44	theme	meaningful	2202:2211	arg1	differences					2213:2223	No meaningful differences	2199:2223	No meaningful differences	2199:2223	No meaningful differences were detected in machine-milking-induced long-term changes between treatment groups.
32008782	8	45	from	effect	1223:1228	arg1	MUOT					1246:1249	MUOT	1246:1249	MUOT	1246:1249	The effect of treatment on MUOT was modified by parity.
32008782	1	46	theme	teat	288:291	arg1	condition					300:308	(3) teat tissue condition	284:308	(3) teat tissue condition	284:308	The objectives were to study the effect of 2 different automatic cluster remover settings on (1) milking characteristics, (2) milk component yields, (3) teat tissue condition, and (4) udder health.
32008782	13	47	dep	ratio	2146:2150	arg1	%					2155:2155	95%	2153:2155	95% confidence interval	2153:2175	The odds of short-term changes to the teat tissue were lower for cows in group ACR1.2 [odds ratio (95% confidence interval) = 0.78 (0.63-0.96)].
32008782	3	48	theme	electronic	708:717	arg1	meters					732:737	electronic on-farm milk meters	708:737	electronic on-farm milk meters	708:737	Treatment consisted of a cluster remover take-off milk flow threshold of 1.2 (ACR1.2) or 0.8 kg/min (ACR0.8) for 57 d. Milking characteristics (milk yield; and milking unit-on time, MUOT) were obtained with electronic on-farm milk meters.
32008782	4	49	theme	cell	830:833	arg1	count					835:839	somatic cell count	822:839	somatic cell count	822:839	Composite milk samples were collected and analyzed for fat, protein, lactose, and somatic cell count.
32008782	12	50	theme	mixed	1963:1967	arg1	model					1969:1973	A generalized linear mixed model	1942:1973	A generalized linear mixed model	1942:1973	A generalized linear mixed model revealed an effect of treatment on machine-milking-induced short-term changes.
32008782	16	51	theme	Future	2575:2580	arg1	studies					2582:2588	Future studies	2575:2588	Future studies	2575:2588	Future studies are warranted to investigate the effect on milk production and udder health over a whole lactation period.
32008782	12	52	from	effect	1987:1992	arg1	changes					2045:2051	machine-milking-induced short-term changes	2010:2051	machine-milking-induced short-term changes	2010:2051	A generalized linear mixed model revealed an effect of treatment on machine-milking-induced short-term changes.
32008782	15	53	theme	somatic	2555:2561	arg1	count					2568:2572	somatic cell count	2555:2572	somatic cell count	2555:2572	Increasing cluster remover take-off milk flow threshold from 0.8 to 1.2 kg/min decreased individual milking duration and alleviated machine-milking-induced short-term changes to the teat tissue without adversely affecting milking performance or somatic cell count.
32008782	12	54	theme	generalized	1944:1954	arg1	model					1969:1973	A generalized linear mixed model	1942:1973	A generalized linear mixed model	1942:1973	A generalized linear mixed model revealed an effect of treatment on machine-milking-induced short-term changes.
32008782	7	55	theme	interval	1121:1128	arg1	%					1108:1108	95%	1106:1108	95% confidence interval	1106:1128	Milk yield (least squares means, 95% confidence interval) was 11.3 (10.9-11.8) and 11.3 (10.8-11.8) kg in groups ACR1.2 and ACR0.8, respectively.
32008782	9	56	theme	287.9-303.6	1503:1513	arg1	s					1516:1516	273.7 (264.9-282.5), 279.1 (269.4-288.8), and 295.7 (287.9-303.6) s	1450:1516	273.7 (264.9-282.5), 279.1 (269.4-288.8), and 295.7 (287.9-303.6) s in group ACR0.8	1450:1532	Milking unit-on time in first-, second-, and ≥third-lactation cows, respectively, was 260.7 (252.0-269.4), 257.8 (247.4-268.1), and 260.2 (252.6-267.9) s in group ACR1.2; and 273.7 (264.9-282.5), 279.1 (269.4-288.8), and 295.7 (287.9-303.6) s in group ACR0.8.
32008782	1	57	dep	yields	276:281	arg1	2					258:258	2	258:258	2	258:258	The objectives were to study the effect of 2 different automatic cluster remover settings on (1) milking characteristics, (2) milk component yields, (3) teat tissue condition, and (4) udder health.
32008782	13	58	theme	teat	2092:2095	arg1	tissue					2097:2102	the teat tissue	2088:2102	the teat tissue	2088:2102	The odds of short-term changes to the teat tissue were lower for cows in group ACR1.2 [odds ratio (95% confidence interval) = 0.78 (0.63-0.96)].
32008782	2	59	theme	field	360:364	arg1	trial					366:370	a randomized controlled field trial	336:370	a randomized controlled field trial	336:370	In a randomized controlled field trial, Holstein cows (n = 689) from 1 commercial dairy farm with a thrice-daily milking schedule were allocated to 2 treatment groups.
32008782	11	60	theme	milk	1754:1757	arg1	kg					1812:1813	kg	1812:1813	kg	1812:1813	Least squares means in groups ACR1.2 and ACR0.8, respectively, were milk fat yield, 0.42 (0.40-0.44) and 0.42 (0.40-0.44) kg; milk protein yield, 0.36 (0.35-0.37) and 0.37 (0.36-0.37) kg; milk lactose yield, 0.61 (0.60-0.63) and 0.63 (0.61-0.64) kg, and linear somatic cell score, 1.9 (1.8-2.0) and 1.9 (1.8-2.0).
32008782	11	60	theme	milk	1754:1757	arg1	0.35-0.37					1780:1788	0.35-0.37	1780:1788	0.35-0.37	1780:1788	Least squares means in groups ACR1.2 and ACR0.8, respectively, were milk fat yield, 0.42 (0.40-0.44) and 0.42 (0.40-0.44) kg; milk protein yield, 0.36 (0.35-0.37) and 0.37 (0.36-0.37) kg; milk lactose yield, 0.61 (0.60-0.63) and 0.63 (0.61-0.64) kg, and linear somatic cell score, 1.9 (1.8-2.0) and 1.9 (1.8-2.0).
32008782	11	60	theme	milk	1754:1757	arg1	yield					1767:1771	milk protein yield	1754:1771	milk protein yield	1754:1771	Least squares means in groups ACR1.2 and ACR0.8, respectively, were milk fat yield, 0.42 (0.40-0.44) and 0.42 (0.40-0.44) kg; milk protein yield, 0.36 (0.35-0.37) and 0.37 (0.36-0.37) kg; milk lactose yield, 0.61 (0.60-0.63) and 0.63 (0.61-0.64) kg, and linear somatic cell score, 1.9 (1.8-2.0) and 1.9 (1.8-2.0).
32008782	6	61	theme	General	948:954	arg1	models					969:974	General linear mixed models	948:974	General linear mixed models	948:974	General linear mixed models demonstrated differences in MUOT, whereas no meaningful differences in milk yield were detected.
32008782	15	62	theme	short-term	2466:2475	arg1	changes					2477:2483	machine-milking-induced short-term changes	2442:2483	machine-milking-induced short-term changes to the teat tissue	2442:2502	Increasing cluster remover take-off milk flow threshold from 0.8 to 1.2 kg/min decreased individual milking duration and alleviated machine-milking-induced short-term changes to the teat tissue without adversely affecting milking performance or somatic cell count.
32008782	3	63	theme	milk	551:554	arg1	threshold					561:569	a cluster remover take-off milk flow threshold	524:569	a cluster remover take-off milk flow threshold of 1.2 (ACR1.2) or 0.8 kg/min (ACR0.8) for 57 d. Milking characteristics (milk yield; and milking unit-on time, MUOT) were obtained with electronic on-farm milk meters	524:737	Treatment consisted of a cluster remover take-off milk flow threshold of 1.2 (ACR1.2) or 0.8 kg/min (ACR0.8) for 57 d. Milking characteristics (milk yield; and milking unit-on time, MUOT) were obtained with electronic on-farm milk meters.
32008782	1	64	theme	different	180:188	arg1	settings					216:223	2 different automatic cluster remover settings	178:223	2 different automatic cluster remover settings	178:223	The objectives were to study the effect of 2 different automatic cluster remover settings on (1) milking characteristics, (2) milk component yields, (3) teat tissue condition, and (4) udder health.
32008782	7	65	theme	squares	1091:1097	arg1	means					1099:1103	least squares means	1085:1103	least squares means	1085:1103	Milk yield (least squares means, 95% confidence interval) was 11.3 (10.9-11.8) and 11.3 (10.8-11.8) kg in groups ACR1.2 and ACR0.8, respectively.
32008782	3	66	theme	remover	534:540	arg1	threshold					561:569	a cluster remover take-off milk flow threshold	524:569	a cluster remover take-off milk flow threshold of 1.2 (ACR1.2) or 0.8 kg/min (ACR0.8) for 57 d. Milking characteristics (milk yield; and milking unit-on time, MUOT) were obtained with electronic on-farm milk meters	524:737	Treatment consisted of a cluster remover take-off milk flow threshold of 1.2 (ACR1.2) or 0.8 kg/min (ACR0.8) for 57 d. Milking characteristics (milk yield; and milking unit-on time, MUOT) were obtained with electronic on-farm milk meters.
32008782	16	67	theme	lactation	2679:2687	arg1	period					2689:2694	a whole lactation period	2671:2694	a whole lactation period	2671:2694	Future studies are warranted to investigate the effect on milk production and udder health over a whole lactation period.
32008782	1	68	theme	cluster	200:206	arg1	settings					216:223	2 different automatic cluster remover settings	178:223	2 different automatic cluster remover settings	178:223	The objectives were to study the effect of 2 different automatic cluster remover settings on (1) milking characteristics, (2) milk component yields, (3) teat tissue condition, and (4) udder health.
32008782	9	69	from	s	1516:1516	arg1	cows					1337:1340	≥third-lactation cows	1320:1340	≥third-lactation cows	1320:1340	Milking unit-on time in first-, second-, and ≥third-lactation cows, respectively, was 260.7 (252.0-269.4), 257.8 (247.4-268.1), and 260.2 (252.6-267.9) s in group ACR1.2; and 273.7 (264.9-282.5), 279.1 (269.4-288.8), and 295.7 (287.9-303.6) s in group ACR0.8.
32008782	9	69	from	s	1516:1516	arg1	group					1521:1525	group ACR0.8	1521:1532	group ACR0.8	1521:1532	Milking unit-on time in first-, second-, and ≥third-lactation cows, respectively, was 260.7 (252.0-269.4), 257.8 (247.4-268.1), and 260.2 (252.6-267.9) s in group ACR1.2; and 273.7 (264.9-282.5), 279.1 (269.4-288.8), and 295.7 (287.9-303.6) s in group ACR0.8.
32008782	9	69	from	s	1516:1516	arg1	group					1432:1436	group ACR1.2	1432:1443	group ACR1.2	1432:1443	Milking unit-on time in first-, second-, and ≥third-lactation cows, respectively, was 260.7 (252.0-269.4), 257.8 (247.4-268.1), and 260.2 (252.6-267.9) s in group ACR1.2; and 273.7 (264.9-282.5), 279.1 (269.4-288.8), and 295.7 (287.9-303.6) s in group ACR0.8.
32008782	9	69	from	s	1516:1516	arg1	second-					1307:1313	second-	1307:1313	second-	1307:1313	Milking unit-on time in first-, second-, and ≥third-lactation cows, respectively, was 260.7 (252.0-269.4), 257.8 (247.4-268.1), and 260.2 (252.6-267.9) s in group ACR1.2; and 273.7 (264.9-282.5), 279.1 (269.4-288.8), and 295.7 (287.9-303.6) s in group ACR0.8.
32008782	9	69	from	s	1516:1516	arg1	first-					1299:1304	first-	1299:1304	first-	1299:1304	Milking unit-on time in first-, second-, and ≥third-lactation cows, respectively, was 260.7 (252.0-269.4), 257.8 (247.4-268.1), and 260.2 (252.6-267.9) s in group ACR1.2; and 273.7 (264.9-282.5), 279.1 (269.4-288.8), and 295.7 (287.9-303.6) s in group ACR0.8.
32008782	7	70	from	11.3	1135:1138	arg1	groups					1179:1184	groups ACR1.2 and ACR0.8	1179:1202	groups	1179:1184	Milk yield (least squares means, 95% confidence interval) was 11.3 (10.9-11.8) and 11.3 (10.8-11.8) kg in groups ACR1.2 and ACR0.8, respectively.
32008782	7	70	from	11.3	1135:1138	arg1	ACR0.8					1197:1202	ACR0.8	1197:1202	ACR0.8	1197:1202	Milk yield (least squares means, 95% confidence interval) was 11.3 (10.9-11.8) and 11.3 (10.8-11.8) kg in groups ACR1.2 and ACR0.8, respectively.
32008782	11	71	theme	cell	1897:1900	arg1	score					1902:1906	linear somatic cell score	1882:1906	linear somatic cell score	1882:1906	Least squares means in groups ACR1.2 and ACR0.8, respectively, were milk fat yield, 0.42 (0.40-0.44) and 0.42 (0.40-0.44) kg; milk protein yield, 0.36 (0.35-0.37) and 0.37 (0.36-0.37) kg; milk lactose yield, 0.61 (0.60-0.63) and 0.63 (0.61-0.64) kg, and linear somatic cell score, 1.9 (1.8-2.0) and 1.9 (1.8-2.0).
32008782	3	72	theme	1.2	574:576	arg1	threshold					561:569	a cluster remover take-off milk flow threshold	524:569	a cluster remover take-off milk flow threshold of 1.2 (ACR1.2) or 0.8 kg/min (ACR0.8) for 57 d. Milking characteristics (milk yield; and milking unit-on time, MUOT) were obtained with electronic on-farm milk meters	524:737	Treatment consisted of a cluster remover take-off milk flow threshold of 1.2 (ACR1.2) or 0.8 kg/min (ACR0.8) for 57 d. Milking characteristics (milk yield; and milking unit-on time, MUOT) were obtained with electronic on-farm milk meters.
32008782	15	73	dep	1.2	2378:2380	arg1	to					2375:2376	to	2375:2376	to	2375:2376	Increasing cluster remover take-off milk flow threshold from 0.8 to 1.2 kg/min decreased individual milking duration and alleviated machine-milking-induced short-term changes to the teat tissue without adversely affecting milking performance or somatic cell count.
32008782	1	74	theme	settings	216:223	arg1	effect					168:173	the effect	164:173	the effect of 2 different automatic cluster remover settings on (1) milking characteristics, (2) milk component yields, (3) teat tissue condition, and (4) udder health	164:330	The objectives were to study the effect of 2 different automatic cluster remover settings on (1) milking characteristics, (2) milk component yields, (3) teat tissue condition, and (4) udder health.
32008782	9	75	theme	247.4-268.1	1389:1399	arg1	s					1427:1427	260.7 (252.0-269.4), 257.8 (247.4-268.1), and 260.2 (252.6-267.9) s	1361:1427	260.7 (252.0-269.4), 257.8 (247.4-268.1), and 260.2 (252.6-267.9) s in group ACR1.2	1361:1443	Milking unit-on time in first-, second-, and ≥third-lactation cows, respectively, was 260.7 (252.0-269.4), 257.8 (247.4-268.1), and 260.2 (252.6-267.9) s in group ACR1.2; and 273.7 (264.9-282.5), 279.1 (269.4-288.8), and 295.7 (287.9-303.6) s in group ACR0.8.
32008782	7	76	theme	Milk	1073:1076	arg1	yield					1078:1082	Milk yield	1073:1082	Milk yield (least squares means, 95% confidence interval)	1073:1129	Milk yield (least squares means, 95% confidence interval) was 11.3 (10.9-11.8) and 11.3 (10.8-11.8) kg in groups ACR1.2 and ACR0.8, respectively.
32008782	11	77	theme	linear	1882:1887	arg1	score					1902:1906	linear somatic cell score	1882:1906	linear somatic cell score	1882:1906	Least squares means in groups ACR1.2 and ACR0.8, respectively, were milk fat yield, 0.42 (0.40-0.44) and 0.42 (0.40-0.44) kg; milk protein yield, 0.36 (0.35-0.37) and 0.37 (0.36-0.37) kg; milk lactose yield, 0.61 (0.60-0.63) and 0.63 (0.61-0.64) kg, and linear somatic cell score, 1.9 (1.8-2.0) and 1.9 (1.8-2.0).
32008782	1	78	dep	health	325:330	arg1	4					316:316	4	316:316	4	316:316	The objectives were to study the effect of 2 different automatic cluster remover settings on (1) milking characteristics, (2) milk component yields, (3) teat tissue condition, and (4) udder health.
32008782	13	79	theme	changes	2077:2083	arg1	odds					2058:2061	The odds	2054:2061	The odds of short-term changes to the teat tissue	2054:2102	The odds of short-term changes to the teat tissue were lower for cows in group ACR1.2 [odds ratio (95% confidence interval) = 0.78 (0.63-0.96)].
32008782	13	79	theme	changes	2077:2083	arg1	lower					2109:2113	lower	2109:2113	lower	2109:2113	The odds of short-term changes to the teat tissue were lower for cows in group ACR1.2 [odds ratio (95% confidence interval) = 0.78 (0.63-0.96)].
32008782	11	80	from	means	1642:1646	arg1	groups					1651:1656	groups ACR1.2 and ACR0.8	1651:1674	groups	1651:1656	Least squares means in groups ACR1.2 and ACR0.8, respectively, were milk fat yield, 0.42 (0.40-0.44) and 0.42 (0.40-0.44) kg; milk protein yield, 0.36 (0.35-0.37) and 0.37 (0.36-0.37) kg; milk lactose yield, 0.61 (0.60-0.63) and 0.63 (0.61-0.64) kg, and linear somatic cell score, 1.9 (1.8-2.0) and 1.9 (1.8-2.0).
32008782	11	80	from	means	1642:1646	arg1	ACR0.8					1669:1674	ACR0.8	1669:1674	ACR0.8	1669:1674	Least squares means in groups ACR1.2 and ACR0.8, respectively, were milk fat yield, 0.42 (0.40-0.44) and 0.42 (0.40-0.44) kg; milk protein yield, 0.36 (0.35-0.37) and 0.37 (0.36-0.37) kg; milk lactose yield, 0.61 (0.60-0.63) and 0.63 (0.61-0.64) kg, and linear somatic cell score, 1.9 (1.8-2.0) and 1.9 (1.8-2.0).
32008782	14	81	located	detected	2230:2237	arg2	differences					2213:2223	No meaningful differences	2199:2223	No meaningful differences	2199:2223	No meaningful differences were detected in machine-milking-induced long-term changes between treatment groups.
32008782	14	81	located	detected	2230:2237	arg1	changes					2276:2282	machine-milking-induced long-term changes	2242:2282	machine-milking-induced long-term changes between treatment groups	2242:2307	No meaningful differences were detected in machine-milking-induced long-term changes between treatment groups.
32008782	10	82	theme	somatic	1608:1614	arg1	score					1621:1625	linear somatic cell score	1601:1625	linear somatic cell score	1601:1625	We detected no meaningful differences in milk component yields or linear somatic cell score.
32008782	12	83	theme	short-term	2034:2043	arg1	changes					2045:2051	machine-milking-induced short-term changes	2010:2051	machine-milking-induced short-term changes	2010:2051	A generalized linear mixed model revealed an effect of treatment on machine-milking-induced short-term changes.
32008782	10	84	theme	meaningful	1550:1559	arg1	differences					1561:1571	no meaningful differences	1547:1571	no meaningful differences	1547:1571	We detected no meaningful differences in milk component yields or linear somatic cell score.
32008782	11	85	theme	milk	1696:1699	arg1	yield					1705:1709	milk fat yield	1696:1709	milk fat yield	1696:1709	Least squares means in groups ACR1.2 and ACR0.8, respectively, were milk fat yield, 0.42 (0.40-0.44) and 0.42 (0.40-0.44) kg; milk protein yield, 0.36 (0.35-0.37) and 0.37 (0.36-0.37) kg; milk lactose yield, 0.61 (0.60-0.63) and 0.63 (0.61-0.64) kg, and linear somatic cell score, 1.9 (1.8-2.0) and 1.9 (1.8-2.0).
32008782	11	85	theme	milk	1696:1699	arg1	kg					1750:1751	kg	1750:1751	kg	1750:1751	Least squares means in groups ACR1.2 and ACR0.8, respectively, were milk fat yield, 0.42 (0.40-0.44) and 0.42 (0.40-0.44) kg; milk protein yield, 0.36 (0.35-0.37) and 0.37 (0.36-0.37) kg; milk lactose yield, 0.61 (0.60-0.63) and 0.63 (0.61-0.64) kg, and linear somatic cell score, 1.9 (1.8-2.0) and 1.9 (1.8-2.0).
32008782	11	85	theme	milk	1696:1699	arg1	0.40-0.44					1718:1726	0.40-0.44	1718:1726	0.40-0.44	1718:1726	Least squares means in groups ACR1.2 and ACR0.8, respectively, were milk fat yield, 0.42 (0.40-0.44) and 0.42 (0.40-0.44) kg; milk protein yield, 0.36 (0.35-0.37) and 0.37 (0.36-0.37) kg; milk lactose yield, 0.61 (0.60-0.63) and 0.63 (0.61-0.64) kg, and linear somatic cell score, 1.9 (1.8-2.0) and 1.9 (1.8-2.0).
32008782	16	86	theme	milk	2633:2636	arg1	production					2638:2647	milk production	2633:2647	milk production	2633:2647	Future studies are warranted to investigate the effect on milk production and udder health over a whole lactation period.
32008782	9	87	theme	252.6-267.9	1414:1424	arg1	s					1427:1427	260.7 (252.0-269.4), 257.8 (247.4-268.1), and 260.2 (252.6-267.9) s	1361:1427	260.7 (252.0-269.4), 257.8 (247.4-268.1), and 260.2 (252.6-267.9) s in group ACR1.2	1361:1443	Milking unit-on time in first-, second-, and ≥third-lactation cows, respectively, was 260.7 (252.0-269.4), 257.8 (247.4-268.1), and 260.2 (252.6-267.9) s in group ACR1.2; and 273.7 (264.9-282.5), 279.1 (269.4-288.8), and 295.7 (287.9-303.6) s in group ACR0.8.
32008782	15	88	theme	individual	2399:2408	arg1	duration					2418:2425	individual milking duration	2399:2425	individual milking duration	2399:2425	Increasing cluster remover take-off milk flow threshold from 0.8 to 1.2 kg/min decreased individual milking duration and alleviated machine-milking-induced short-term changes to the teat tissue without adversely affecting milking performance or somatic cell count.
32008782	11	89	theme	fat	1701:1703	arg1	yield					1705:1709	milk fat yield	1696:1709	milk fat yield	1696:1709	Least squares means in groups ACR1.2 and ACR0.8, respectively, were milk fat yield, 0.42 (0.40-0.44) and 0.42 (0.40-0.44) kg; milk protein yield, 0.36 (0.35-0.37) and 0.37 (0.36-0.37) kg; milk lactose yield, 0.61 (0.60-0.63) and 0.63 (0.61-0.64) kg, and linear somatic cell score, 1.9 (1.8-2.0) and 1.9 (1.8-2.0).
32008782	11	89	theme	fat	1701:1703	arg1	kg					1750:1751	kg	1750:1751	kg	1750:1751	Least squares means in groups ACR1.2 and ACR0.8, respectively, were milk fat yield, 0.42 (0.40-0.44) and 0.42 (0.40-0.44) kg; milk protein yield, 0.36 (0.35-0.37) and 0.37 (0.36-0.37) kg; milk lactose yield, 0.61 (0.60-0.63) and 0.63 (0.61-0.64) kg, and linear somatic cell score, 1.9 (1.8-2.0) and 1.9 (1.8-2.0).
32008782	11	89	theme	fat	1701:1703	arg1	0.40-0.44					1718:1726	0.40-0.44	1718:1726	0.40-0.44	1718:1726	Least squares means in groups ACR1.2 and ACR0.8, respectively, were milk fat yield, 0.42 (0.40-0.44) and 0.42 (0.40-0.44) kg; milk protein yield, 0.36 (0.35-0.37) and 0.37 (0.36-0.37) kg; milk lactose yield, 0.61 (0.60-0.63) and 0.63 (0.61-0.64) kg, and linear somatic cell score, 1.9 (1.8-2.0) and 1.9 (1.8-2.0).
32008782	2	90	theme	dairy	415:419	arg1	farm					421:424	1 commercial dairy farm	402:424	1 commercial dairy farm with a thrice-daily milking schedule	402:461	In a randomized controlled field trial, Holstein cows (n = 689) from 1 commercial dairy farm with a thrice-daily milking schedule were allocated to 2 treatment groups.
32008782	6	91	theme	mixed	963:967	arg1	models					969:974	General linear mixed models	948:974	General linear mixed models	948:974	General linear mixed models demonstrated differences in MUOT, whereas no meaningful differences in milk yield were detected.
32008782	16	92	theme	udder	2653:2657	arg1	health					2659:2664	udder health	2653:2664	udder health	2653:2664	Future studies are warranted to investigate the effect on milk production and udder health over a whole lactation period.
32008782	11	93	theme	Least	1628:1632	arg1	squares					1634:1640	Least squares	1628:1640	Least squares means in groups ACR1.2 and ACR0.8	1628:1674	Least squares means in groups ACR1.2 and ACR0.8, respectively, were milk fat yield, 0.42 (0.40-0.44) and 0.42 (0.40-0.44) kg; milk protein yield, 0.36 (0.35-0.37) and 0.37 (0.36-0.37) kg; milk lactose yield, 0.61 (0.60-0.63) and 0.63 (0.61-0.64) kg, and linear somatic cell score, 1.9 (1.8-2.0) and 1.9 (1.8-2.0).
32008782	4	94	theme	milk	750:753	arg1	samples					755:761	Composite milk samples	740:761	Composite milk samples	740:761	Composite milk samples were collected and analyzed for fat, protein, lactose, and somatic cell count.
32008782	8	95	theme	treatment	1233:1241	arg1	effect					1223:1228	The effect	1219:1228	The effect of treatment on MUOT	1219:1249	The effect of treatment on MUOT was modified by parity.
32008782	15	96	theme	milk	2346:2349	arg1	threshold					2356:2364	cluster remover take-off milk flow threshold	2321:2364	cluster remover take-off milk flow threshold	2321:2364	Increasing cluster remover take-off milk flow threshold from 0.8 to 1.2 kg/min decreased individual milking duration and alleviated machine-milking-induced short-term changes to the teat tissue without adversely affecting milking performance or somatic cell count.
32008782	5	97	theme	teat	902:905	arg1	condition					914:922	the teat tissue condition	898:922	the teat tissue condition	898:922	Machine-milking-induced short- and long-term changes to the teat tissue condition were assessed visually.
32008782	2	98	theme	thrice-daily	433:444	arg1	schedule					454:461	a thrice-daily milking schedule	431:461	a thrice-daily milking schedule	431:461	In a randomized controlled field trial, Holstein cows (n = 689) from 1 commercial dairy farm with a thrice-daily milking schedule were allocated to 2 treatment groups.
32008782	6	99	theme	milk	1047:1050	arg1	yield					1052:1056	milk yield	1047:1056	milk yield	1047:1056	General linear mixed models demonstrated differences in MUOT, whereas no meaningful differences in milk yield were detected.
32008782	15	100	theme	remover	2329:2335	arg1	threshold					2356:2364	cluster remover take-off milk flow threshold	2321:2364	cluster remover take-off milk flow threshold	2321:2364	Increasing cluster remover take-off milk flow threshold from 0.8 to 1.2 kg/min decreased individual milking duration and alleviated machine-milking-induced short-term changes to the teat tissue without adversely affecting milking performance or somatic cell count.
32008782	5	101	theme	short-	866:871	arg1	changes					887:893	Machine-milking-induced short- and long-term changes	842:893	Machine-milking-induced short- and long-term changes to the teat tissue condition	842:922	Machine-milking-induced short- and long-term changes to the teat tissue condition were assessed visually.
32008782	1	102	from	effect	168:173	arg1	characteristics					240:254	(1) milking characteristics	228:254	(1) milking characteristics	228:254	The objectives were to study the effect of 2 different automatic cluster remover settings on (1) milking characteristics, (2) milk component yields, (3) teat tissue condition, and (4) udder health.
32008782	1	102	from	effect	168:173	arg1	condition					300:308	(3) teat tissue condition	284:308	(3) teat tissue condition	284:308	The objectives were to study the effect of 2 different automatic cluster remover settings on (1) milking characteristics, (2) milk component yields, (3) teat tissue condition, and (4) udder health.
32008782	1	102	from	effect	168:173	arg1	yields					276:281	(2) milk component yields	257:281	(2) milk component yields	257:281	The objectives were to study the effect of 2 different automatic cluster remover settings on (1) milking characteristics, (2) milk component yields, (3) teat tissue condition, and (4) udder health.
32008782	1	102	from	effect	168:173	arg1	health					325:330	(4) udder health	315:330	(4) udder health	315:330	The objectives were to study the effect of 2 different automatic cluster remover settings on (1) milking characteristics, (2) milk component yields, (3) teat tissue condition, and (4) udder health.
32008782	3	103	theme	milk	645:648	arg1	characteristics					628:642	characteristics	628:642	characteristics (milk yield; and milking unit-on time, MUOT)	628:687	Treatment consisted of a cluster remover take-off milk flow threshold of 1.2 (ACR1.2) or 0.8 kg/min (ACR0.8) for 57 d. Milking characteristics (milk yield; and milking unit-on time, MUOT) were obtained with electronic on-farm milk meters.
32008782	3	103	theme	milk	645:648	arg1	yield					650:654	milk yield	645:654	milk yield	645:654	Treatment consisted of a cluster remover take-off milk flow threshold of 1.2 (ACR1.2) or 0.8 kg/min (ACR0.8) for 57 d. Milking characteristics (milk yield; and milking unit-on time, MUOT) were obtained with electronic on-farm milk meters.
32008782	0	104	theme	cluster	52:58	arg1	settings					68:75	automatic cluster remover settings	42:75	automatic cluster remover settings	42:75	A randomized trial to study the effect of automatic cluster remover settings on milking performance, teat condition, and udder health.
32008782	5	105	theme	long-term	877:885	arg1	changes					887:893	Machine-milking-induced short- and long-term changes	842:893	Machine-milking-induced short- and long-term changes to the teat tissue condition	842:922	Machine-milking-induced short- and long-term changes to the teat tissue condition were assessed visually.
32008782	9	106	theme	unit-on	1283:1289	arg1	time					1291:1294	Milking unit-on time	1275:1294	Milking unit-on time	1275:1294	Milking unit-on time in first-, second-, and ≥third-lactation cows, respectively, was 260.7 (252.0-269.4), 257.8 (247.4-268.1), and 260.2 (252.6-267.9) s in group ACR1.2; and 273.7 (264.9-282.5), 279.1 (269.4-288.8), and 295.7 (287.9-303.6) s in group ACR0.8.
32008782	9	107	theme	264.9-282.5	1457:1467	arg1	s					1516:1516	273.7 (264.9-282.5), 279.1 (269.4-288.8), and 295.7 (287.9-303.6) s	1450:1516	273.7 (264.9-282.5), 279.1 (269.4-288.8), and 295.7 (287.9-303.6) s in group ACR0.8	1450:1532	Milking unit-on time in first-, second-, and ≥third-lactation cows, respectively, was 260.7 (252.0-269.4), 257.8 (247.4-268.1), and 260.2 (252.6-267.9) s in group ACR1.2; and 273.7 (264.9-282.5), 279.1 (269.4-288.8), and 295.7 (287.9-303.6) s in group ACR0.8.
32008782	1	108	dep	characteristics	240:254	arg1	1					229:229	1	229:229	1	229:229	The objectives were to study the effect of 2 different automatic cluster remover settings on (1) milking characteristics, (2) milk component yields, (3) teat tissue condition, and (4) udder health.
32008782	14	109	theme	long-term	2266:2274	arg1	changes					2276:2282	machine-milking-induced long-term changes	2242:2282	machine-milking-induced long-term changes between treatment groups	2242:2307	No meaningful differences were detected in machine-milking-induced long-term changes between treatment groups.
32008782	0	110	theme	settings	68:75	arg1	effect					32:37	the effect	28:37	the effect of automatic cluster remover settings on milking performance, teat condition, and udder health	28:132	A randomized trial to study the effect of automatic cluster remover settings on milking performance, teat condition, and udder health.
32008782	2	111	theme	Holstein	373:380	arg1	n					388:388	n = 689	388:394	n = 689	388:394	In a randomized controlled field trial, Holstein cows (n = 689) from 1 commercial dairy farm with a thrice-daily milking schedule were allocated to 2 treatment groups.
32008782	2	111	theme	Holstein	373:380	arg1	cows					382:385	Holstein cows	373:385	Holstein cows (n = 689) from 1 commercial dairy farm with a thrice-daily milking schedule	373:461	In a randomized controlled field trial, Holstein cows (n = 689) from 1 commercial dairy farm with a thrice-daily milking schedule were allocated to 2 treatment groups.
32008782	3	112	theme	milking	661:667	arg1	time					677:680	milking unit-on time	661:680	milking unit-on time	661:680	Treatment consisted of a cluster remover take-off milk flow threshold of 1.2 (ACR1.2) or 0.8 kg/min (ACR0.8) for 57 d. Milking characteristics (milk yield; and milking unit-on time, MUOT) were obtained with electronic on-farm milk meters.
32008782	3	112	theme	milking	661:667	arg1	characteristics					628:642	characteristics	628:642	characteristics (milk yield; and milking unit-on time, MUOT)	628:687	Treatment consisted of a cluster remover take-off milk flow threshold of 1.2 (ACR1.2) or 0.8 kg/min (ACR0.8) for 57 d. Milking characteristics (milk yield; and milking unit-on time, MUOT) were obtained with electronic on-farm milk meters.
32008782	0	113	theme	milking	80:86	arg1	performance					88:98	milking performance	80:98	milking performance	80:98	A randomized trial to study the effect of automatic cluster remover settings on milking performance, teat condition, and udder health.
32008782	13	114	theme	[odds	2140:2144	arg1	ratio					2146:2150	[odds ratio	2140:2150	[odds ratio (95% confidence interval)	2140:2176	The odds of short-term changes to the teat tissue were lower for cows in group ACR1.2 [odds ratio (95% confidence interval) = 0.78 (0.63-0.96)].
32008782	7	115	dep	means	1099:1103	arg1	%					1108:1108	95%	1106:1108	95% confidence interval	1106:1128	Milk yield (least squares means, 95% confidence interval) was 11.3 (10.9-11.8) and 11.3 (10.8-11.8) kg in groups ACR1.2 and ACR0.8, respectively.
32008782	14	116	theme	machine-milking-induced	2242:2264	arg1	changes					2276:2282	machine-milking-induced long-term changes	2242:2282	machine-milking-induced long-term changes between treatment groups	2242:2307	No meaningful differences were detected in machine-milking-induced long-term changes between treatment groups.
32008782	2	117	theme	=	390:390	arg1	n					388:388	n = 689	388:394	n = 689	388:394	In a randomized controlled field trial, Holstein cows (n = 689) from 1 commercial dairy farm with a thrice-daily milking schedule were allocated to 2 treatment groups.
32008782	2	117	theme	=	390:390	arg1	cows					382:385	Holstein cows	373:385	Holstein cows (n = 689) from 1 commercial dairy farm with a thrice-daily milking schedule	373:461	In a randomized controlled field trial, Holstein cows (n = 689) from 1 commercial dairy farm with a thrice-daily milking schedule were allocated to 2 treatment groups.
32008782	11	118	theme	milk	1816:1819	arg1	yield					1829:1833	milk lactose yield	1816:1833	milk lactose yield	1816:1833	Least squares means in groups ACR1.2 and ACR0.8, respectively, were milk fat yield, 0.42 (0.40-0.44) and 0.42 (0.40-0.44) kg; milk protein yield, 0.36 (0.35-0.37) and 0.37 (0.36-0.37) kg; milk lactose yield, 0.61 (0.60-0.63) and 0.63 (0.61-0.64) kg, and linear somatic cell score, 1.9 (1.8-2.0) and 1.9 (1.8-2.0).
32008782	0	119	theme	randomized	2:11	arg1	trial					13:17	A randomized trial	0:17	A randomized trial	0:17	A randomized trial to study the effect of automatic cluster remover settings on milking performance, teat condition, and udder health.
32008782	1	120	theme	tissue	293:298	arg1	condition					300:308	(3) teat tissue condition	284:308	(3) teat tissue condition	284:308	The objectives were to study the effect of 2 different automatic cluster remover settings on (1) milking characteristics, (2) milk component yields, (3) teat tissue condition, and (4) udder health.
32008782	9	121	theme	≥third-lactation	1320:1335	arg1	cows					1337:1340	≥third-lactation cows	1320:1340	≥third-lactation cows	1320:1340	Milking unit-on time in first-, second-, and ≥third-lactation cows, respectively, was 260.7 (252.0-269.4), 257.8 (247.4-268.1), and 260.2 (252.6-267.9) s in group ACR1.2; and 273.7 (264.9-282.5), 279.1 (269.4-288.8), and 295.7 (287.9-303.6) s in group ACR0.8.
32008782	3	122	theme	on-farm	719:725	arg1	meters					732:737	electronic on-farm milk meters	708:737	electronic on-farm milk meters	708:737	Treatment consisted of a cluster remover take-off milk flow threshold of 1.2 (ACR1.2) or 0.8 kg/min (ACR0.8) for 57 d. Milking characteristics (milk yield; and milking unit-on time, MUOT) were obtained with electronic on-farm milk meters.
32008782	4	123	theme	somatic	822:828	arg1	count					835:839	somatic cell count	822:839	somatic cell count	822:839	Composite milk samples were collected and analyzed for fat, protein, lactose, and somatic cell count.
32008782	12	124	theme	linear	1956:1961	arg1	model					1969:1973	A generalized linear mixed model	1942:1973	A generalized linear mixed model	1942:1973	A generalized linear mixed model revealed an effect of treatment on machine-milking-induced short-term changes.
32008782	1	125	theme	milking	232:238	arg1	characteristics					240:254	(1) milking characteristics	228:254	(1) milking characteristics	228:254	The objectives were to study the effect of 2 different automatic cluster remover settings on (1) milking characteristics, (2) milk component yields, (3) teat tissue condition, and (4) udder health.
32008782	7	126	dep	yield	1078:1082	arg1	means					1099:1103	least squares means	1085:1103	least squares means	1085:1103	Milk yield (least squares means, 95% confidence interval) was 11.3 (10.9-11.8) and 11.3 (10.8-11.8) kg in groups ACR1.2 and ACR0.8, respectively.
32008782	15	127	theme	milking	2532:2538	arg1	performance					2540:2550	milking performance	2532:2550	milking performance	2532:2550	Increasing cluster remover take-off milk flow threshold from 0.8 to 1.2 kg/min decreased individual milking duration and alleviated machine-milking-induced short-term changes to the teat tissue without adversely affecting milking performance or somatic cell count.
32008782	6	128	from	differences	989:999	arg1	MUOT					1004:1007	MUOT	1004:1007	MUOT	1004:1007	General linear mixed models demonstrated differences in MUOT, whereas no meaningful differences in milk yield were detected.
32008782	3	129	dep	1.2	574:576	arg1	kg/min					594:599	kg/min (ACR0.8)	594:608	kg/min (ACR0.8)	594:608	Treatment consisted of a cluster remover take-off milk flow threshold of 1.2 (ACR1.2) or 0.8 kg/min (ACR0.8) for 57 d. Milking characteristics (milk yield; and milking unit-on time, MUOT) were obtained with electronic on-farm milk meters.
32008782	15	130	theme	cell	2563:2566	arg1	count					2568:2572	somatic cell count	2555:2572	somatic cell count	2555:2572	Increasing cluster remover take-off milk flow threshold from 0.8 to 1.2 kg/min decreased individual milking duration and alleviated machine-milking-induced short-term changes to the teat tissue without adversely affecting milking performance or somatic cell count.
32008782	1	131	dep	condition	300:308	arg1	3					285:285	3	285:285	3	285:285	The objectives were to study the effect of 2 different automatic cluster remover settings on (1) milking characteristics, (2) milk component yields, (3) teat tissue condition, and (4) udder health.
32008782	11	132	theme	lactose	1821:1827	arg1	yield					1829:1833	milk lactose yield	1816:1833	milk lactose yield	1816:1833	Least squares means in groups ACR1.2 and ACR0.8, respectively, were milk fat yield, 0.42 (0.40-0.44) and 0.42 (0.40-0.44) kg; milk protein yield, 0.36 (0.35-0.37) and 0.37 (0.36-0.37) kg; milk lactose yield, 0.61 (0.60-0.63) and 0.63 (0.61-0.64) kg, and linear somatic cell score, 1.9 (1.8-2.0) and 1.9 (1.8-2.0).
32008782	7	133	dep	11.3	1135:1138	arg1	kg					1173:1174	kg	1173:1174	kg	1173:1174	Milk yield (least squares means, 95% confidence interval) was 11.3 (10.9-11.8) and 11.3 (10.8-11.8) kg in groups ACR1.2 and ACR0.8, respectively.
32008782	6	134	theme	linear	956:961	arg1	models					969:974	General linear mixed models	948:974	General linear mixed models	948:974	General linear mixed models demonstrated differences in MUOT, whereas no meaningful differences in milk yield were detected.
32008782	2	135	theme	controlled	349:358	arg1	trial					366:370	a randomized controlled field trial	336:370	a randomized controlled field trial	336:370	In a randomized controlled field trial, Holstein cows (n = 689) from 1 commercial dairy farm with a thrice-daily milking schedule were allocated to 2 treatment groups.
32008782	3	136	theme	take-off	542:549	arg1	threshold					561:569	a cluster remover take-off milk flow threshold	524:569	a cluster remover take-off milk flow threshold of 1.2 (ACR1.2) or 0.8 kg/min (ACR0.8) for 57 d. Milking characteristics (milk yield; and milking unit-on time, MUOT) were obtained with electronic on-farm milk meters	524:737	Treatment consisted of a cluster remover take-off milk flow threshold of 1.2 (ACR1.2) or 0.8 kg/min (ACR0.8) for 57 d. Milking characteristics (milk yield; and milking unit-on time, MUOT) were obtained with electronic on-farm milk meters.
32008782	16	137	theme	whole	2673:2677	arg1	period					2689:2694	a whole lactation period	2671:2694	a whole lactation period	2671:2694	Future studies are warranted to investigate the effect on milk production and udder health over a whole lactation period.
32008782	1	138	theme	milk	261:264	arg1	yields					276:281	(2) milk component yields	257:281	(2) milk component yields	257:281	The objectives were to study the effect of 2 different automatic cluster remover settings on (1) milking characteristics, (2) milk component yields, (3) teat tissue condition, and (4) udder health.
32008782	9	139	theme	252.0-269.4	1368:1378	arg1	s					1427:1427	260.7 (252.0-269.4), 257.8 (247.4-268.1), and 260.2 (252.6-267.9) s	1361:1427	260.7 (252.0-269.4), 257.8 (247.4-268.1), and 260.2 (252.6-267.9) s in group ACR1.2	1361:1443	Milking unit-on time in first-, second-, and ≥third-lactation cows, respectively, was 260.7 (252.0-269.4), 257.8 (247.4-268.1), and 260.2 (252.6-267.9) s in group ACR1.2; and 273.7 (264.9-282.5), 279.1 (269.4-288.8), and 295.7 (287.9-303.6) s in group ACR0.8.
32008782	3	140	theme	cluster	526:532	arg1	threshold					561:569	a cluster remover take-off milk flow threshold	524:569	a cluster remover take-off milk flow threshold of 1.2 (ACR1.2) or 0.8 kg/min (ACR0.8) for 57 d. Milking characteristics (milk yield; and milking unit-on time, MUOT) were obtained with electronic on-farm milk meters	524:737	Treatment consisted of a cluster remover take-off milk flow threshold of 1.2 (ACR1.2) or 0.8 kg/min (ACR0.8) for 57 d. Milking characteristics (milk yield; and milking unit-on time, MUOT) were obtained with electronic on-farm milk meters.
32008782	2	141	with	farm	421:424	arg1	schedule					454:461	a thrice-daily milking schedule	431:461	a thrice-daily milking schedule	431:461	In a randomized controlled field trial, Holstein cows (n = 689) from 1 commercial dairy farm with a thrice-daily milking schedule were allocated to 2 treatment groups.
32008782	1	142	theme	automatic	190:198	arg1	settings					216:223	2 different automatic cluster remover settings	178:223	2 different automatic cluster remover settings	178:223	The objectives were to study the effect of 2 different automatic cluster remover settings on (1) milking characteristics, (2) milk component yields, (3) teat tissue condition, and (4) udder health.
32008782	2	143	from	farm	421:424	arg1	n					388:388	n = 689	388:394	n = 689	388:394	In a randomized controlled field trial, Holstein cows (n = 689) from 1 commercial dairy farm with a thrice-daily milking schedule were allocated to 2 treatment groups.
32008782	2	143	from	farm	421:424	arg1	cows					382:385	Holstein cows	373:385	Holstein cows (n = 689) from 1 commercial dairy farm with a thrice-daily milking schedule	373:461	In a randomized controlled field trial, Holstein cows (n = 689) from 1 commercial dairy farm with a thrice-daily milking schedule were allocated to 2 treatment groups.
32008782	2	144	theme	randomized	338:347	arg1	trial					366:370	a randomized controlled field trial	336:370	a randomized controlled field trial	336:370	In a randomized controlled field trial, Holstein cows (n = 689) from 1 commercial dairy farm with a thrice-daily milking schedule were allocated to 2 treatment groups.
32008782	15	145	theme	flow	2351:2354	arg1	threshold					2356:2364	cluster remover take-off milk flow threshold	2321:2364	cluster remover take-off milk flow threshold	2321:2364	Increasing cluster remover take-off milk flow threshold from 0.8 to 1.2 kg/min decreased individual milking duration and alleviated machine-milking-induced short-term changes to the teat tissue without adversely affecting milking performance or somatic cell count.
32008782	1	146	theme	remover	208:214	arg1	settings					216:223	2 different automatic cluster remover settings	178:223	2 different automatic cluster remover settings	178:223	The objectives were to study the effect of 2 different automatic cluster remover settings on (1) milking characteristics, (2) milk component yields, (3) teat tissue condition, and (4) udder health.
32628542	4	0	theme	new	595:597	arg1	Resolution					570:579	Integrative MultiOmics Pathway Resolution	539:579	Integrative MultiOmics Pathway Resolution (IMPRes)	539:588	Integrative MultiOmics Pathway Resolution (IMPRes) is a new bioinformatic tool that enables simultaneous time-series analysis of more than two groups to identify pathways and molecular connections.
32628542	4	0	theme	new	595:597	arg1	tool					613:616	a new bioinformatic tool	593:616	a new bioinformatic tool that enables simultaneous time-series analysis of more than two groups to identify pathways and molecular connections	593:734	Integrative MultiOmics Pathway Resolution (IMPRes) is a new bioinformatic tool that enables simultaneous time-series analysis of more than two groups to identify pathways and molecular connections.
32628542	10	1	theme	increased	1500:1508	arg1	expression					1510:1519	expression	1510:1519	expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR	1510:1735	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	13	2	theme	podocytes	2084:2092	arg1	remodeling					2046:2055	FFSS-induced glycocalyx remodeling	2022:2055	FFSS-induced glycocalyx remodeling	2022:2055	Identifying the "proteoglycans in cancer" and "galactose metabolism" pathways has generated a lead to study the significance of FFSS-induced glycocalyx remodeling and possible detachment of podocytes from the glomerular matrix.
32628542	13	2	theme	podocytes	2084:2092	arg1	detachment					2070:2079	possible detachment	2061:2079	possible detachment of podocytes from the glomerular matrix	2061:2119	Identifying the "proteoglycans in cancer" and "galactose metabolism" pathways has generated a lead to study the significance of FFSS-induced glycocalyx remodeling and possible detachment of podocytes from the glomerular matrix.
32628542	10	3	theme	target	1556:1561	arg1	expression					1510:1519	expression	1510:1519	expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR	1510:1735	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	10	3	theme	target	1556:1561	arg1	AKT3					1741:1744	AKT3	1741:1744	AKT3	1741:1744	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	1	4	theme	ultrafiltrate	102:114	arg1	flow					116:119	The ultrafiltrate flow	98:119	The ultrafiltrate flow over the major processes and cell body	98:158	The ultrafiltrate flow over the major processes and cell body generates fluid flow shear stress (FFSS) on podocytes.
32628542	9	5	theme	IMPRes	1383:1388	arg1	analysis					1390:1397	IMPRes analysis	1383:1397	IMPRes analysis	1383:1397	A custom-designed PCR array validated 60.7% of the genes predicted by IMPRes analysis, including genes for the above-named pathways.
32628542	4	6	theme	groups	682:687	arg1	analysis					656:663	simultaneous time-series analysis	631:663	simultaneous time-series analysis of more than two groups	631:687	Integrative MultiOmics Pathway Resolution (IMPRes) is a new bioinformatic tool that enables simultaneous time-series analysis of more than two groups to identify pathways and molecular connections.
32628542	2	7	theme	Hyperfiltration-associated	215:240	arg1	increase					242:249	Hyperfiltration-associated increase	215:249	Hyperfiltration-associated increase in FFSS	215:257	Hyperfiltration-associated increase in FFSS can lead to podocyte injury and detachment.
32628542	8	8	theme	proteoglycans	1231:1243	arg1	"					1254:1254	"proteoglycans in cancer"	1230:1254	"proteoglycans in cancer"	1230:1254	We investigated the "proteoglycans in cancer" and "galactose metabolism" pathways predicted by IMPRes.
32628542	10	9	theme	rapamycin	1566:1574	arg1	mTOR					1732:1735	unchanged total mTOR	1716:1735	unchanged total mTOR	1716:1735	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	10	9	theme	rapamycin	1566:1574	arg1	Galm					1660:1663	Galm	1660:1663	Galm	1660:1663	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	10	9	theme	rapamycin	1566:1574	arg1	hexokinase					1594:1603	hexokinase II	1594:1606	hexokinase II	1594:1606	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	10	9	theme	rapamycin	1566:1574	arg1	B4galt1					1702:1708	B4galt1	1702:1708	B4galt1	1702:1708	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	10	9	theme	rapamycin	1566:1574	arg1	Erbb2					1631:1635	decreased total Erbb2	1615:1635	decreased total Erbb2	1615:1635	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	10	9	theme	rapamycin	1566:1574	arg1	Hk2					1609:1611	Hk2	1609:1611	Hk2	1609:1611	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	10	9	theme	rapamycin	1566:1574	arg1	target					1556:1561	phospho-mammalian target	1538:1561	phospho-mammalian target of rapamycin (mTOR)	1538:1581	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	10	9	theme	rapamycin	1566:1574	arg1	CD44					1584:1587	CD44	1584:1587	CD44	1584:1587	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	10	9	theme	rapamycin	1566:1574	arg1	mutarotase					1648:1657	galactose mutarotase	1638:1657	galactose mutarotase (Galm)	1638:1664	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	10	9	theme	rapamycin	1566:1574	arg1	β-1,4-galactosyltransferase					1671:1697	β-1,4-galactosyltransferase 1	1671:1699	β-1,4-galactosyltransferase 1	1671:1699	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	10	9	theme	rapamycin	1566:1574	arg1	phosho-Erbb2					1524:1535	phosho-Erbb2	1524:1535	phosho-Erbb2	1524:1535	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	13	10	theme	remodeling	2046:2055	arg1	significance					2006:2017	the significance	2002:2017	the significance of FFSS-induced glycocalyx remodeling and possible detachment of podocytes from the glomerular matrix	2002:2119	Identifying the "proteoglycans in cancer" and "galactose metabolism" pathways has generated a lead to study the significance of FFSS-induced glycocalyx remodeling and possible detachment of podocytes from the glomerular matrix.
32628542	12	11	theme	novel	1878:1882	arg1	pathways					1884:1891	novel pathways	1878:1891	novel pathways	1878:1891	This study demonstrates the potential of the IMPRes method to identify novel pathways.
32628542	10	12	theme	hexokinase	1594:1603	arg1	expression					1510:1519	expression	1510:1519	expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR	1510:1735	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	10	12	theme	hexokinase	1594:1603	arg1	AKT3					1741:1744	AKT3	1741:1744	AKT3	1741:1744	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	5	13	theme	present	744:750	arg1	study					752:756	the present study	740:756	the present study	740:756	In the present study, we used previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)], EP2 (Ptger2), and β1-catenin (Ctnnb1) as "seed genes" from an array data set of four groups analyzed over a time course.
32628542	13	14	theme	possible	2061:2068	arg1	detachment					2070:2079	possible detachment	2061:2079	possible detachment of podocytes from the glomerular matrix	2061:2119	Identifying the "proteoglycans in cancer" and "galactose metabolism" pathways has generated a lead to study the significance of FFSS-induced glycocalyx remodeling and possible detachment of podocytes from the glomerular matrix.
32628542	10	15	theme	mTOR	1732:1735	arg1	expression					1510:1519	expression	1510:1519	expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR	1510:1735	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	10	15	theme	mTOR	1732:1735	arg1	AKT3					1741:1744	AKT3	1741:1744	AKT3	1741:1744	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	10	16	theme	unchanged	1716:1724	arg1	mTOR					1732:1735	unchanged total mTOR	1716:1735	unchanged total mTOR	1716:1735	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	8	17	theme	galactose	1261:1269	arg1	"					1281:1281	"galactose metabolism"	1260:1281	"galactose metabolism"	1260:1281	We investigated the "proteoglycans in cancer" and "galactose metabolism" pathways predicted by IMPRes.
32628542	3	18	from	upregulation	343:354	arg1	podocytes					433:441	podocytes	433:441	podocytes	433:441	Previously, we showed that FFSS-induced upregulation of the cyclooxygenase 2 (COX2)-PGE2-prostaglandin E receptor 2 (EP2) axis in podocytes activates Akt-glycogen synthase kinase-3β-β-catenin and MAPK/ERK signaling in response to FFSS.
32628542	1	19	theme	cell	150:153	arg1	body					155:158	cell body	150:158	cell body	150:158	The ultrafiltrate flow over the major processes and cell body generates fluid flow shear stress (FFSS) on podocytes.
32628542	6	20	theme	seed	973:976	arg1	genes					978:982	The 3 seed genes	967:982	The 3 seed genes	967:982	The 3 seed genes shared 7 pathways and 50 genes of 14 pathways and 89 genes identified by IMPRes.
32628542	3	21	theme	synthase	466:473	arg1	kinase-3β-β-catenin					475:493	Akt-glycogen synthase kinase-3β-β-catenin	453:493	Akt-glycogen synthase kinase-3β-β-catenin	453:493	Previously, we showed that FFSS-induced upregulation of the cyclooxygenase 2 (COX2)-PGE2-prostaglandin E receptor 2 (EP2) axis in podocytes activates Akt-glycogen synthase kinase-3β-β-catenin and MAPK/ERK signaling in response to FFSS.
32628542	8	22	theme	"	1281:1281	arg1	pathways					1283:1290	the "proteoglycans in cancer" and "galactose metabolism" pathways	1226:1290	the "proteoglycans in cancer" and "galactose metabolism" pathways predicted by IMPRes	1226:1310	We investigated the "proteoglycans in cancer" and "galactose metabolism" pathways predicted by IMPRes.
32628542	0	23	theme	stress-treated	72:85	arg1	podocytes					87:95	fluid flow shear stress-treated podocytes	55:95	fluid flow shear stress-treated podocytes	55:95	Upregulated proteoglycan-related signaling pathways in fluid flow shear stress-treated podocytes.
32628542	8	24	from	proteoglycans	1231:1243	arg1	cancer					1248:1253	cancer	1248:1253	cancer	1248:1253	We investigated the "proteoglycans in cancer" and "galactose metabolism" pathways predicted by IMPRes.
32628542	13	25	from	proteoglycans	1911:1923	arg1	"					1934:1934	cancer"	1928:1934	cancer"	1928:1934	Identifying the "proteoglycans in cancer" and "galactose metabolism" pathways has generated a lead to study the significance of FFSS-induced glycocalyx remodeling and possible detachment of podocytes from the glomerular matrix.
32628542	13	25	from	proteoglycans	1911:1923	arg1	"					1961:1961	"galactose metabolism"	1940:1961	"galactose metabolism"	1940:1961	Identifying the "proteoglycans in cancer" and "galactose metabolism" pathways has generated a lead to study the significance of FFSS-induced glycocalyx remodeling and possible detachment of podocytes from the glomerular matrix.
32628542	5	26	theme	[prostaglandin-endoperoxide	797:823	arg1	β1-catenin					864:873	β1-catenin	864:873	β1-catenin (Ctnnb1)	864:882	In the present study, we used previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)], EP2 (Ptger2), and β1-catenin (Ctnnb1) as "seed genes" from an array data set of four groups analyzed over a time course.
32628542	5	26	theme	[prostaglandin-endoperoxide	797:823	arg1	synthase					825:832	previously characterized COX2 [prostaglandin-endoperoxide synthase 2	767:834	previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)]	767:843	In the present study, we used previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)], EP2 (Ptger2), and β1-catenin (Ctnnb1) as "seed genes" from an array data set of four groups analyzed over a time course.
32628542	5	26	theme	[prostaglandin-endoperoxide	797:823	arg1	Ptgs2					837:841	Ptgs2	837:841	Ptgs2	837:841	In the present study, we used previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)], EP2 (Ptger2), and β1-catenin (Ctnnb1) as "seed genes" from an array data set of four groups analyzed over a time course.
32628542	5	26	theme	[prostaglandin-endoperoxide	797:823	arg1	genes					893:897	"seed genes	887:897	"seed genes" from an array data set of four groups analyzed over a time course	887:964	In the present study, we used previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)], EP2 (Ptger2), and β1-catenin (Ctnnb1) as "seed genes" from an array data set of four groups analyzed over a time course.
32628542	5	26	theme	[prostaglandin-endoperoxide	797:823	arg1	EP2					846:848	EP2	846:848	EP2 (Ptger2)	846:857	In the present study, we used previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)], EP2 (Ptger2), and β1-catenin (Ctnnb1) as "seed genes" from an array data set of four groups analyzed over a time course.
32628542	13	27	theme	FFSS-induced	2022:2033	arg1	remodeling					2046:2055	FFSS-induced glycocalyx remodeling	2022:2055	FFSS-induced glycocalyx remodeling	2022:2055	Identifying the "proteoglycans in cancer" and "galactose metabolism" pathways has generated a lead to study the significance of FFSS-induced glycocalyx remodeling and possible detachment of podocytes from the glomerular matrix.
32628542	1	28	theme	flow	176:179	arg1	FFSS					195:198	FFSS	195:198	FFSS	195:198	The ultrafiltrate flow over the major processes and cell body generates fluid flow shear stress (FFSS) on podocytes.
32628542	1	28	theme	flow	176:179	arg1	stress					187:192	fluid flow shear stress	170:192	fluid flow shear stress (FFSS) on podocytes	170:212	The ultrafiltrate flow over the major processes and cell body generates fluid flow shear stress (FFSS) on podocytes.
32628542	7	29	theme	mechanotransduction	1153:1171	arg1	signaling					1173:1181	mechanotransduction signaling	1153:1181	mechanotransduction signaling in FFSS-treated podocytes	1153:1207	A composite of signaling pathways highlighted the temporal molecular connections during mechanotransduction signaling in FFSS-treated podocytes.
32628542	12	30	theme	method	1859:1864	arg1	potential					1835:1843	the potential	1831:1843	the potential of the IMPRes method to identify novel pathways	1831:1891	This study demonstrates the potential of the IMPRes method to identify novel pathways.
32628542	5	31	theme	groups	931:936	arg1	set					919:921	an array data set	905:921	an array data set of four groups analyzed over a time course	905:964	In the present study, we used previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)], EP2 (Ptger2), and β1-catenin (Ctnnb1) as "seed genes" from an array data set of four groups analyzed over a time course.
32628542	5	31	theme	groups	931:936	arg1	groups					931:936	four groups	926:936	four groups analyzed over a time course	926:964	In the present study, we used previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)], EP2 (Ptger2), and β1-catenin (Ctnnb1) as "seed genes" from an array data set of four groups analyzed over a time course.
32628542	2	32	from	increase	242:249	arg1	FFSS					254:257	FFSS	254:257	FFSS	254:257	Hyperfiltration-associated increase in FFSS can lead to podocyte injury and detachment.
32628542	3	33	theme	-PGE2-prostaglandin	386:404	arg1	axis					425:428	the cyclooxygenase 2 (COX2)-PGE2-prostaglandin E receptor 2 (EP2) axis	359:428	the cyclooxygenase 2 (COX2)-PGE2-prostaglandin E receptor 2 (EP2) axis	359:428	Previously, we showed that FFSS-induced upregulation of the cyclooxygenase 2 (COX2)-PGE2-prostaglandin E receptor 2 (EP2) axis in podocytes activates Akt-glycogen synthase kinase-3β-β-catenin and MAPK/ERK signaling in response to FFSS.
32628542	7	34	theme	temporal	1115:1122	arg1	connections					1134:1144	the temporal molecular connections	1111:1144	the temporal molecular connections	1111:1144	A composite of signaling pathways highlighted the temporal molecular connections during mechanotransduction signaling in FFSS-treated podocytes.
32628542	5	35	theme	data	914:917	arg1	set					919:921	an array data set	905:921	an array data set of four groups analyzed over a time course	905:964	In the present study, we used previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)], EP2 (Ptger2), and β1-catenin (Ctnnb1) as "seed genes" from an array data set of four groups analyzed over a time course.
32628542	5	35	theme	data	914:917	arg1	groups					931:936	four groups	926:936	four groups analyzed over a time course	926:964	In the present study, we used previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)], EP2 (Ptger2), and β1-catenin (Ctnnb1) as "seed genes" from an array data set of four groups analyzed over a time course.
32628542	13	36	dep	"	1934:1934	arg1	pathways					1963:1970	pathways	1963:1970	pathways	1963:1970	Identifying the "proteoglycans in cancer" and "galactose metabolism" pathways has generated a lead to study the significance of FFSS-induced glycocalyx remodeling and possible detachment of podocytes from the glomerular matrix.
32628542	10	37	theme	total	1625:1629	arg1	Erbb2					1631:1635	decreased total Erbb2	1615:1635	decreased total Erbb2	1615:1635	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	10	37	theme	total	1625:1629	arg1	B4galt1					1702:1708	B4galt1	1702:1708	B4galt1	1702:1708	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	0	38	theme	signaling	33:41	arg1	pathways					43:50	proteoglycan-related signaling pathways	12:50	proteoglycan-related signaling pathways in fluid flow shear stress-treated podocytes	12:95	Upregulated proteoglycan-related signaling pathways in fluid flow shear stress-treated podocytes.
32628542	10	39	theme	Further	1446:1452	arg1	validation					1454:1463	Further validation	1446:1463	Further validation using Western blot analysis	1446:1491	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	7	40	theme	signaling	1080:1088	arg1	pathways					1090:1097	signaling pathways	1080:1097	signaling pathways	1080:1097	A composite of signaling pathways highlighted the temporal molecular connections during mechanotransduction signaling in FFSS-treated podocytes.
32628542	9	41	theme	custom-designed	1315:1329	arg1	array					1335:1339	A custom-designed PCR array	1313:1339	A custom-designed PCR array	1313:1339	A custom-designed PCR array validated 60.7% of the genes predicted by IMPRes analysis, including genes for the above-named pathways.
32628542	3	42	theme	receptor	408:415	arg1	axis					425:428	the cyclooxygenase 2 (COX2)-PGE2-prostaglandin E receptor 2 (EP2) axis	359:428	the cyclooxygenase 2 (COX2)-PGE2-prostaglandin E receptor 2 (EP2) axis	359:428	Previously, we showed that FFSS-induced upregulation of the cyclooxygenase 2 (COX2)-PGE2-prostaglandin E receptor 2 (EP2) axis in podocytes activates Akt-glycogen synthase kinase-3β-β-catenin and MAPK/ERK signaling in response to FFSS.
32628542	4	43	theme	Integrative	539:549	arg1	tool					613:616	a new bioinformatic tool	593:616	a new bioinformatic tool that enables simultaneous time-series analysis of more than two groups to identify pathways and molecular connections	593:734	Integrative MultiOmics Pathway Resolution (IMPRes) is a new bioinformatic tool that enables simultaneous time-series analysis of more than two groups to identify pathways and molecular connections.
32628542	4	43	theme	Integrative	539:549	arg1	IMPRes					582:587	IMPRes	582:587	IMPRes	582:587	Integrative MultiOmics Pathway Resolution (IMPRes) is a new bioinformatic tool that enables simultaneous time-series analysis of more than two groups to identify pathways and molecular connections.
32628542	4	43	theme	Integrative	539:549	arg1	Resolution					570:579	Integrative MultiOmics Pathway Resolution	539:579	Integrative MultiOmics Pathway Resolution (IMPRes)	539:588	Integrative MultiOmics Pathway Resolution (IMPRes) is a new bioinformatic tool that enables simultaneous time-series analysis of more than two groups to identify pathways and molecular connections.
32628542	5	44	theme	characterized	778:790	arg1	β1-catenin					864:873	β1-catenin	864:873	β1-catenin (Ctnnb1)	864:882	In the present study, we used previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)], EP2 (Ptger2), and β1-catenin (Ctnnb1) as "seed genes" from an array data set of four groups analyzed over a time course.
32628542	5	44	theme	characterized	778:790	arg1	synthase					825:832	previously characterized COX2 [prostaglandin-endoperoxide synthase 2	767:834	previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)]	767:843	In the present study, we used previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)], EP2 (Ptger2), and β1-catenin (Ctnnb1) as "seed genes" from an array data set of four groups analyzed over a time course.
32628542	5	44	theme	characterized	778:790	arg1	Ptgs2					837:841	Ptgs2	837:841	Ptgs2	837:841	In the present study, we used previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)], EP2 (Ptger2), and β1-catenin (Ctnnb1) as "seed genes" from an array data set of four groups analyzed over a time course.
32628542	5	44	theme	characterized	778:790	arg1	genes					893:897	"seed genes	887:897	"seed genes" from an array data set of four groups analyzed over a time course	887:964	In the present study, we used previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)], EP2 (Ptger2), and β1-catenin (Ctnnb1) as "seed genes" from an array data set of four groups analyzed over a time course.
32628542	5	44	theme	characterized	778:790	arg1	EP2					846:848	EP2	846:848	EP2 (Ptger2)	846:857	In the present study, we used previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)], EP2 (Ptger2), and β1-catenin (Ctnnb1) as "seed genes" from an array data set of four groups analyzed over a time course.
32628542	13	45	theme	galactose	1941:1949	arg1	metabolism					1951:1960	galactose metabolism	1941:1960	"galactose metabolism"	1940:1961	Identifying the "proteoglycans in cancer" and "galactose metabolism" pathways has generated a lead to study the significance of FFSS-induced glycocalyx remodeling and possible detachment of podocytes from the glomerular matrix.
32628542	0	46	theme	flow	61:64	arg1	podocytes					87:95	fluid flow shear stress-treated podocytes	55:95	fluid flow shear stress-treated podocytes	55:95	Upregulated proteoglycan-related signaling pathways in fluid flow shear stress-treated podocytes.
32628542	4	47	theme	Pathway	562:568	arg1	tool					613:616	a new bioinformatic tool	593:616	a new bioinformatic tool that enables simultaneous time-series analysis of more than two groups to identify pathways and molecular connections	593:734	Integrative MultiOmics Pathway Resolution (IMPRes) is a new bioinformatic tool that enables simultaneous time-series analysis of more than two groups to identify pathways and molecular connections.
32628542	4	47	theme	Pathway	562:568	arg1	IMPRes					582:587	IMPRes	582:587	IMPRes	582:587	Integrative MultiOmics Pathway Resolution (IMPRes) is a new bioinformatic tool that enables simultaneous time-series analysis of more than two groups to identify pathways and molecular connections.
32628542	4	47	theme	Pathway	562:568	arg1	Resolution					570:579	Integrative MultiOmics Pathway Resolution	539:579	Integrative MultiOmics Pathway Resolution (IMPRes)	539:588	Integrative MultiOmics Pathway Resolution (IMPRes) is a new bioinformatic tool that enables simultaneous time-series analysis of more than two groups to identify pathways and molecular connections.
32628542	10	48	theme	blot	1479:1482	arg1	analysis					1484:1491	Western blot analysis	1471:1491	Western blot analysis	1471:1491	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	13	49	theme	glomerular	2103:2112	arg1	matrix					2114:2119	the glomerular matrix	2099:2119	the glomerular matrix	2099:2119	Identifying the "proteoglycans in cancer" and "galactose metabolism" pathways has generated a lead to study the significance of FFSS-induced glycocalyx remodeling and possible detachment of podocytes from the glomerular matrix.
32628542	5	50	theme	time	954:957	arg1	course					959:964	a time course	952:964	a time course	952:964	In the present study, we used previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)], EP2 (Ptger2), and β1-catenin (Ctnnb1) as "seed genes" from an array data set of four groups analyzed over a time course.
32628542	10	51	theme	phosho-Erbb2	1524:1535	arg1	expression					1510:1519	expression	1510:1519	expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR	1510:1735	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	10	51	theme	phosho-Erbb2	1524:1535	arg1	AKT3					1741:1744	AKT3	1741:1744	AKT3	1741:1744	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	13	52	theme	cancer	1928:1933	arg1	"					1934:1934	cancer"	1928:1934	cancer"	1928:1934	Identifying the "proteoglycans in cancer" and "galactose metabolism" pathways has generated a lead to study the significance of FFSS-induced glycocalyx remodeling and possible detachment of podocytes from the glomerular matrix.
32628542	4	53	theme	simultaneous	631:642	arg1	analysis					656:663	simultaneous time-series analysis	631:663	simultaneous time-series analysis of more than two groups	631:687	Integrative MultiOmics Pathway Resolution (IMPRes) is a new bioinformatic tool that enables simultaneous time-series analysis of more than two groups to identify pathways and molecular connections.
32628542	13	54	from	significance	2006:2017	arg1	matrix					2114:2119	the glomerular matrix	2099:2119	the glomerular matrix	2099:2119	Identifying the "proteoglycans in cancer" and "galactose metabolism" pathways has generated a lead to study the significance of FFSS-induced glycocalyx remodeling and possible detachment of podocytes from the glomerular matrix.
32628542	9	55	theme	genes	1364:1368	arg1	genes					1410:1414	genes	1410:1414	genes for the above-named pathways	1410:1443	A custom-designed PCR array validated 60.7% of the genes predicted by IMPRes analysis, including genes for the above-named pathways.
32628542	9	55	theme	genes	1364:1368	arg1	%					1355:1355	60.7%	1351:1355	60.7% of the genes predicted by IMPRes analysis, including genes for the above-named pathways	1351:1443	A custom-designed PCR array validated 60.7% of the genes predicted by IMPRes analysis, including genes for the above-named pathways.
32628542	9	55	theme	genes	1364:1368	arg1	genes					1364:1368	the genes	1360:1368	the genes	1360:1368	A custom-designed PCR array validated 60.7% of the genes predicted by IMPRes analysis, including genes for the above-named pathways.
32628542	6	56	theme	pathways	1021:1028	arg1	pathways					993:1000	7 pathways	991:1000	7 pathways	991:1000	The 3 seed genes shared 7 pathways and 50 genes of 14 pathways and 89 genes identified by IMPRes.
32628542	6	56	theme	pathways	1021:1028	arg1	genes					1009:1013	50 genes	1006:1013	50 genes	1006:1013	The 3 seed genes shared 7 pathways and 50 genes of 14 pathways and 89 genes identified by IMPRes.
32628542	12	57	theme	IMPRes	1852:1857	arg1	method					1859:1864	the IMPRes method	1848:1864	the IMPRes method	1848:1864	This study demonstrates the potential of the IMPRes method to identify novel pathways.
32628542	4	58	theme	bioinformatic	599:611	arg1	Resolution					570:579	Integrative MultiOmics Pathway Resolution	539:579	Integrative MultiOmics Pathway Resolution (IMPRes)	539:588	Integrative MultiOmics Pathway Resolution (IMPRes) is a new bioinformatic tool that enables simultaneous time-series analysis of more than two groups to identify pathways and molecular connections.
32628542	4	58	theme	bioinformatic	599:611	arg1	tool					613:616	a new bioinformatic tool	593:616	a new bioinformatic tool that enables simultaneous time-series analysis of more than two groups to identify pathways and molecular connections	593:734	Integrative MultiOmics Pathway Resolution (IMPRes) is a new bioinformatic tool that enables simultaneous time-series analysis of more than two groups to identify pathways and molecular connections.
32628542	10	59	theme	phospho-mammalian	1538:1554	arg1	target					1556:1561	phospho-mammalian target	1538:1561	phospho-mammalian target of rapamycin (mTOR)	1538:1581	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	1	60	theme	shear	181:185	arg1	FFSS					195:198	FFSS	195:198	FFSS	195:198	The ultrafiltrate flow over the major processes and cell body generates fluid flow shear stress (FFSS) on podocytes.
32628542	1	60	theme	shear	181:185	arg1	stress					187:192	fluid flow shear stress	170:192	fluid flow shear stress (FFSS) on podocytes	170:212	The ultrafiltrate flow over the major processes and cell body generates fluid flow shear stress (FFSS) on podocytes.
32628542	13	61	from	matrix	2114:2119	arg1	significance					2006:2017	the significance	2002:2017	the significance of FFSS-induced glycocalyx remodeling and possible detachment of podocytes from the glomerular matrix	2002:2119	Identifying the "proteoglycans in cancer" and "galactose metabolism" pathways has generated a lead to study the significance of FFSS-induced glycocalyx remodeling and possible detachment of podocytes from the glomerular matrix.
32628542	13	61	from	matrix	2114:2119	arg1	remodeling					2046:2055	FFSS-induced glycocalyx remodeling	2022:2055	FFSS-induced glycocalyx remodeling	2022:2055	Identifying the "proteoglycans in cancer" and "galactose metabolism" pathways has generated a lead to study the significance of FFSS-induced glycocalyx remodeling and possible detachment of podocytes from the glomerular matrix.
32628542	13	61	from	matrix	2114:2119	arg1	detachment					2070:2079	possible detachment	2061:2079	possible detachment of podocytes from the glomerular matrix	2061:2119	Identifying the "proteoglycans in cancer" and "galactose metabolism" pathways has generated a lead to study the significance of FFSS-induced glycocalyx remodeling and possible detachment of podocytes from the glomerular matrix.
32628542	5	62	theme	seed	888:891	arg1	β1-catenin					864:873	β1-catenin	864:873	β1-catenin (Ctnnb1)	864:882	In the present study, we used previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)], EP2 (Ptger2), and β1-catenin (Ctnnb1) as "seed genes" from an array data set of four groups analyzed over a time course.
32628542	5	62	theme	seed	888:891	arg1	synthase					825:832	previously characterized COX2 [prostaglandin-endoperoxide synthase 2	767:834	previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)]	767:843	In the present study, we used previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)], EP2 (Ptger2), and β1-catenin (Ctnnb1) as "seed genes" from an array data set of four groups analyzed over a time course.
32628542	5	62	theme	seed	888:891	arg1	genes					893:897	"seed genes	887:897	"seed genes" from an array data set of four groups analyzed over a time course	887:964	In the present study, we used previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)], EP2 (Ptger2), and β1-catenin (Ctnnb1) as "seed genes" from an array data set of four groups analyzed over a time course.
32628542	5	62	theme	seed	888:891	arg1	EP2					846:848	EP2	846:848	EP2 (Ptger2)	846:857	In the present study, we used previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)], EP2 (Ptger2), and β1-catenin (Ctnnb1) as "seed genes" from an array data set of four groups analyzed over a time course.
32628542	11	63	theme	reported	1789:1796	arg1	results					1798:1804	our previously reported results	1774:1804	our previously reported results	1774:1804	These findings corroborate our previously reported results.
32628542	9	64	theme	above-named	1424:1434	arg1	pathways					1436:1443	the above-named pathways	1420:1443	the above-named pathways	1420:1443	A custom-designed PCR array validated 60.7% of the genes predicted by IMPRes analysis, including genes for the above-named pathways.
32628542	10	65	theme	CD44	1584:1587	arg1	expression					1510:1519	expression	1510:1519	expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR	1510:1735	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	10	65	theme	CD44	1584:1587	arg1	AKT3					1741:1744	AKT3	1741:1744	AKT3	1741:1744	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	13	66	theme	detachment	2070:2079	arg1	significance					2006:2017	the significance	2002:2017	the significance of FFSS-induced glycocalyx remodeling and possible detachment of podocytes from the glomerular matrix	2002:2119	Identifying the "proteoglycans in cancer" and "galactose metabolism" pathways has generated a lead to study the significance of FFSS-induced glycocalyx remodeling and possible detachment of podocytes from the glomerular matrix.
32628542	10	67	theme	total	1726:1730	arg1	mTOR					1732:1735	unchanged total mTOR	1716:1735	unchanged total mTOR	1716:1735	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	1	68	theme	major	130:134	arg1	processes					136:144	the major processes	126:144	the major processes	126:144	The ultrafiltrate flow over the major processes and cell body generates fluid flow shear stress (FFSS) on podocytes.
32628542	7	69	from	signaling	1173:1181	arg1	podocytes					1199:1207	FFSS-treated podocytes	1186:1207	FFSS-treated podocytes	1186:1207	A composite of signaling pathways highlighted the temporal molecular connections during mechanotransduction signaling in FFSS-treated podocytes.
32628542	8	70	theme	"	1254:1254	arg1	pathways					1283:1290	the "proteoglycans in cancer" and "galactose metabolism" pathways	1226:1290	the "proteoglycans in cancer" and "galactose metabolism" pathways predicted by IMPRes	1226:1310	We investigated the "proteoglycans in cancer" and "galactose metabolism" pathways predicted by IMPRes.
32628542	2	71	theme	podocyte	271:278	arg1	injury					280:285	podocyte injury	271:285	podocyte injury	271:285	Hyperfiltration-associated increase in FFSS can lead to podocyte injury and detachment.
32628542	7	72	theme	FFSS-treated	1186:1197	arg1	podocytes					1199:1207	FFSS-treated podocytes	1186:1207	FFSS-treated podocytes	1186:1207	A composite of signaling pathways highlighted the temporal molecular connections during mechanotransduction signaling in FFSS-treated podocytes.
32628542	13	73	theme	glycocalyx	2035:2044	arg1	remodeling					2046:2055	FFSS-induced glycocalyx remodeling	2022:2055	FFSS-induced glycocalyx remodeling	2022:2055	Identifying the "proteoglycans in cancer" and "galactose metabolism" pathways has generated a lead to study the significance of FFSS-induced glycocalyx remodeling and possible detachment of podocytes from the glomerular matrix.
32628542	0	74	from	pathways	43:50	arg1	podocytes					87:95	fluid flow shear stress-treated podocytes	55:95	fluid flow shear stress-treated podocytes	55:95	Upregulated proteoglycan-related signaling pathways in fluid flow shear stress-treated podocytes.
32628542	8	75	theme	metabolism	1271:1280	arg1	"					1281:1281	"galactose metabolism"	1260:1281	"galactose metabolism"	1260:1281	We investigated the "proteoglycans in cancer" and "galactose metabolism" pathways predicted by IMPRes.
32628542	5	76	theme	COX2	792:795	arg1	β1-catenin					864:873	β1-catenin	864:873	β1-catenin (Ctnnb1)	864:882	In the present study, we used previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)], EP2 (Ptger2), and β1-catenin (Ctnnb1) as "seed genes" from an array data set of four groups analyzed over a time course.
32628542	5	76	theme	COX2	792:795	arg1	synthase					825:832	previously characterized COX2 [prostaglandin-endoperoxide synthase 2	767:834	previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)]	767:843	In the present study, we used previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)], EP2 (Ptger2), and β1-catenin (Ctnnb1) as "seed genes" from an array data set of four groups analyzed over a time course.
32628542	5	76	theme	COX2	792:795	arg1	Ptgs2					837:841	Ptgs2	837:841	Ptgs2	837:841	In the present study, we used previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)], EP2 (Ptger2), and β1-catenin (Ctnnb1) as "seed genes" from an array data set of four groups analyzed over a time course.
32628542	5	76	theme	COX2	792:795	arg1	genes					893:897	"seed genes	887:897	"seed genes" from an array data set of four groups analyzed over a time course	887:964	In the present study, we used previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)], EP2 (Ptger2), and β1-catenin (Ctnnb1) as "seed genes" from an array data set of four groups analyzed over a time course.
32628542	5	76	theme	COX2	792:795	arg1	EP2					846:848	EP2	846:848	EP2 (Ptger2)	846:857	In the present study, we used previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)], EP2 (Ptger2), and β1-catenin (Ctnnb1) as "seed genes" from an array data set of four groups analyzed over a time course.
32628542	5	77	used	used	762:765	arg2	we					759:760	we	759:760	we	759:760	In the present study, we used previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)], EP2 (Ptger2), and β1-catenin (Ctnnb1) as "seed genes" from an array data set of four groups analyzed over a time course.
32628542	1	78	from	stress	187:192	arg1	podocytes					204:212	podocytes	204:212	podocytes	204:212	The ultrafiltrate flow over the major processes and cell body generates fluid flow shear stress (FFSS) on podocytes.
32628542	1	79	theme	fluid	170:174	arg1	FFSS					195:198	FFSS	195:198	FFSS	195:198	The ultrafiltrate flow over the major processes and cell body generates fluid flow shear stress (FFSS) on podocytes.
32628542	1	79	theme	fluid	170:174	arg1	stress					187:192	fluid flow shear stress	170:192	fluid flow shear stress (FFSS) on podocytes	170:212	The ultrafiltrate flow over the major processes and cell body generates fluid flow shear stress (FFSS) on podocytes.
32628542	3	80	theme	FFSS-induced	330:341	arg1	upregulation					343:354	FFSS-induced upregulation	330:354	FFSS-induced upregulation of the cyclooxygenase 2 (COX2)-PGE2-prostaglandin E receptor 2 (EP2) axis in podocytes	330:441	Previously, we showed that FFSS-induced upregulation of the cyclooxygenase 2 (COX2)-PGE2-prostaglandin E receptor 2 (EP2) axis in podocytes activates Akt-glycogen synthase kinase-3β-β-catenin and MAPK/ERK signaling in response to FFSS.
32628542	4	81	theme	molecular	714:722	arg1	connections					724:734	molecular connections	714:734	molecular connections	714:734	Integrative MultiOmics Pathway Resolution (IMPRes) is a new bioinformatic tool that enables simultaneous time-series analysis of more than two groups to identify pathways and molecular connections.
32628542	10	82	theme	Erbb2	1631:1635	arg1	expression					1510:1519	expression	1510:1519	expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR	1510:1735	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	10	82	theme	Erbb2	1631:1635	arg1	AKT3					1741:1744	AKT3	1741:1744	AKT3	1741:1744	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	7	83	theme	molecular	1124:1132	arg1	connections					1134:1144	the temporal molecular connections	1111:1144	the temporal molecular connections	1111:1144	A composite of signaling pathways highlighted the temporal molecular connections during mechanotransduction signaling in FFSS-treated podocytes.
32628542	3	84	theme	cyclooxygenase	363:376	arg1	axis					425:428	the cyclooxygenase 2 (COX2)-PGE2-prostaglandin E receptor 2 (EP2) axis	359:428	the cyclooxygenase 2 (COX2)-PGE2-prostaglandin E receptor 2 (EP2) axis	359:428	Previously, we showed that FFSS-induced upregulation of the cyclooxygenase 2 (COX2)-PGE2-prostaglandin E receptor 2 (EP2) axis in podocytes activates Akt-glycogen synthase kinase-3β-β-catenin and MAPK/ERK signaling in response to FFSS.
32628542	0	85	theme	proteoglycan-related	12:31	arg1	pathways					43:50	proteoglycan-related signaling pathways	12:50	proteoglycan-related signaling pathways in fluid flow shear stress-treated podocytes	12:95	Upregulated proteoglycan-related signaling pathways in fluid flow shear stress-treated podocytes.
32628542	5	86	theme	array	908:912	arg1	set					919:921	an array data set	905:921	an array data set of four groups analyzed over a time course	905:964	In the present study, we used previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)], EP2 (Ptger2), and β1-catenin (Ctnnb1) as "seed genes" from an array data set of four groups analyzed over a time course.
32628542	5	86	theme	array	908:912	arg1	groups					931:936	four groups	926:936	four groups analyzed over a time course	926:964	In the present study, we used previously characterized COX2 [prostaglandin-endoperoxide synthase 2 (Ptgs2)], EP2 (Ptger2), and β1-catenin (Ctnnb1) as "seed genes" from an array data set of four groups analyzed over a time course.
32628542	3	87	theme	E	406:406	arg1	axis					425:428	the cyclooxygenase 2 (COX2)-PGE2-prostaglandin E receptor 2 (EP2) axis	359:428	the cyclooxygenase 2 (COX2)-PGE2-prostaglandin E receptor 2 (EP2) axis	359:428	Previously, we showed that FFSS-induced upregulation of the cyclooxygenase 2 (COX2)-PGE2-prostaglandin E receptor 2 (EP2) axis in podocytes activates Akt-glycogen synthase kinase-3β-β-catenin and MAPK/ERK signaling in response to FFSS.
32628542	3	88	theme	axis	425:428	arg1	upregulation					343:354	FFSS-induced upregulation	330:354	FFSS-induced upregulation of the cyclooxygenase 2 (COX2)-PGE2-prostaglandin E receptor 2 (EP2) axis in podocytes	330:441	Previously, we showed that FFSS-induced upregulation of the cyclooxygenase 2 (COX2)-PGE2-prostaglandin E receptor 2 (EP2) axis in podocytes activates Akt-glycogen synthase kinase-3β-β-catenin and MAPK/ERK signaling in response to FFSS.
32628542	7	89	theme	pathways	1090:1097	arg1	composite					1067:1075	A composite	1065:1075	A composite of signaling pathways	1065:1097	A composite of signaling pathways highlighted the temporal molecular connections during mechanotransduction signaling in FFSS-treated podocytes.
32628542	7	89	theme	pathways	1090:1097	arg1	pathways					1090:1097	signaling pathways	1080:1097	signaling pathways	1080:1097	A composite of signaling pathways highlighted the temporal molecular connections during mechanotransduction signaling in FFSS-treated podocytes.
32628542	3	90	dep	FFSS	533:536	arg1	response					521:528	response	521:528	response	521:528	Previously, we showed that FFSS-induced upregulation of the cyclooxygenase 2 (COX2)-PGE2-prostaglandin E receptor 2 (EP2) axis in podocytes activates Akt-glycogen synthase kinase-3β-β-catenin and MAPK/ERK signaling in response to FFSS.
32628542	10	91	theme	decreased	1615:1623	arg1	Erbb2					1631:1635	decreased total Erbb2	1615:1635	decreased total Erbb2	1615:1635	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	10	91	theme	decreased	1615:1623	arg1	B4galt1					1702:1708	B4galt1	1702:1708	B4galt1	1702:1708	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	0	92	theme	fluid	55:59	arg1	podocytes					87:95	fluid flow shear stress-treated podocytes	55:95	fluid flow shear stress-treated podocytes	55:95	Upregulated proteoglycan-related signaling pathways in fluid flow shear stress-treated podocytes.
32628542	3	93	theme	EP2	420:422	arg1	axis					425:428	the cyclooxygenase 2 (COX2)-PGE2-prostaglandin E receptor 2 (EP2) axis	359:428	the cyclooxygenase 2 (COX2)-PGE2-prostaglandin E receptor 2 (EP2) axis	359:428	Previously, we showed that FFSS-induced upregulation of the cyclooxygenase 2 (COX2)-PGE2-prostaglandin E receptor 2 (EP2) axis in podocytes activates Akt-glycogen synthase kinase-3β-β-catenin and MAPK/ERK signaling in response to FFSS.
32628542	4	94	theme	MultiOmics	551:560	arg1	tool					613:616	a new bioinformatic tool	593:616	a new bioinformatic tool that enables simultaneous time-series analysis of more than two groups to identify pathways and molecular connections	593:734	Integrative MultiOmics Pathway Resolution (IMPRes) is a new bioinformatic tool that enables simultaneous time-series analysis of more than two groups to identify pathways and molecular connections.
32628542	4	94	theme	MultiOmics	551:560	arg1	IMPRes					582:587	IMPRes	582:587	IMPRes	582:587	Integrative MultiOmics Pathway Resolution (IMPRes) is a new bioinformatic tool that enables simultaneous time-series analysis of more than two groups to identify pathways and molecular connections.
32628542	4	94	theme	MultiOmics	551:560	arg1	Resolution					570:579	Integrative MultiOmics Pathway Resolution	539:579	Integrative MultiOmics Pathway Resolution (IMPRes)	539:588	Integrative MultiOmics Pathway Resolution (IMPRes) is a new bioinformatic tool that enables simultaneous time-series analysis of more than two groups to identify pathways and molecular connections.
32628542	3	95	theme	Akt-glycogen	453:464	arg1	kinase-3β-β-catenin					475:493	Akt-glycogen synthase kinase-3β-β-catenin	453:493	Akt-glycogen synthase kinase-3β-β-catenin	453:493	Previously, we showed that FFSS-induced upregulation of the cyclooxygenase 2 (COX2)-PGE2-prostaglandin E receptor 2 (EP2) axis in podocytes activates Akt-glycogen synthase kinase-3β-β-catenin and MAPK/ERK signaling in response to FFSS.
32628542	0	96	theme	shear	66:70	arg1	podocytes					87:95	fluid flow shear stress-treated podocytes	55:95	fluid flow shear stress-treated podocytes	55:95	Upregulated proteoglycan-related signaling pathways in fluid flow shear stress-treated podocytes.
32628542	9	97	theme	PCR	1331:1333	arg1	array					1335:1339	A custom-designed PCR array	1313:1339	A custom-designed PCR array	1313:1339	A custom-designed PCR array validated 60.7% of the genes predicted by IMPRes analysis, including genes for the above-named pathways.
32628542	13	98	theme	metabolism	1951:1960	arg1	"					1961:1961	"galactose metabolism"	1940:1961	"galactose metabolism"	1940:1961	Identifying the "proteoglycans in cancer" and "galactose metabolism" pathways has generated a lead to study the significance of FFSS-induced glycocalyx remodeling and possible detachment of podocytes from the glomerular matrix.
32628542	10	99	theme	galactose	1638:1646	arg1	Galm					1660:1663	Galm	1660:1663	Galm	1660:1663	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	10	99	theme	galactose	1638:1646	arg1	mutarotase					1648:1657	galactose mutarotase	1638:1657	galactose mutarotase (Galm)	1638:1664	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	10	100	theme	Western	1471:1477	arg1	analysis					1484:1491	Western blot analysis	1471:1491	Western blot analysis	1471:1491	Further validation using Western blot analysis showed increased expression of phosho-Erbb2, phospho-mammalian target of rapamycin (mTOR), CD44, and hexokinase II (Hk2); decreased total Erbb2, galactose mutarotase (Galm), and β-1,4-galactosyltransferase 1 (B4galt1); and unchanged total mTOR and AKT3.
32628542	3	101	theme	MAPK/ERK	499:506	arg1	signaling					508:516	MAPK/ERK signaling	499:516	MAPK/ERK signaling	499:516	Previously, we showed that FFSS-induced upregulation of the cyclooxygenase 2 (COX2)-PGE2-prostaglandin E receptor 2 (EP2) axis in podocytes activates Akt-glycogen synthase kinase-3β-β-catenin and MAPK/ERK signaling in response to FFSS.
32628542	6	102	theme	genes	1037:1041	arg1	pathways					993:1000	7 pathways	991:1000	7 pathways	991:1000	The 3 seed genes shared 7 pathways and 50 genes of 14 pathways and 89 genes identified by IMPRes.
32628542	6	102	theme	genes	1037:1041	arg1	genes					1009:1013	50 genes	1006:1013	50 genes	1006:1013	The 3 seed genes shared 7 pathways and 50 genes of 14 pathways and 89 genes identified by IMPRes.
32628542	4	103	theme	time-series	644:654	arg1	analysis					656:663	simultaneous time-series analysis	631:663	simultaneous time-series analysis of more than two groups	631:687	Integrative MultiOmics Pathway Resolution (IMPRes) is a new bioinformatic tool that enables simultaneous time-series analysis of more than two groups to identify pathways and molecular connections.
31917232	5	0	theme	study	675:679	arg1	objective					657:665	The objective	653:665	The objective of this study	653:679	The objective of this study was to investigate the role of solute size, and tissue structure and composition on molecular diffusion in meniscus tissue.
31917232	6	1	theme	direction-dependent	880:898	arg1	diffusivity					900:910	the direction-dependent diffusivity	876:910	the direction-dependent diffusivity in human meniscus of six different molecular probes of size ranging from ∼300Da to 150,000Da	876:1003	DESIGN Using a custom FRAP technique developed in our lab, we measured the direction-dependent diffusivity in human meniscus of six different molecular probes of size ranging from ∼300Da to 150,000Da.
31917232	2	2	theme	interstitial	349:360	arg1	diffusion					324:332	diffusion	324:332	diffusion	324:332	Due to the tissue's limited vascularization, nutrients and other molecular signals spread through the extracellular matrix via diffusion or convection (interstitial fluid flow).
31917232	2	2	theme	interstitial	349:360	arg1	flow					368:371	interstitial fluid flow	349:371	interstitial fluid flow	349:371	Due to the tissue's limited vascularization, nutrients and other molecular signals spread through the extracellular matrix via diffusion or convection (interstitial fluid flow).
31917232	0	3	theme	tissue	77:82	arg1	structure					84:92	tissue structure	77:92	tissue structure	77:92	Molecular and macromolecular diffusion in human meniscus: relationships with tissue structure and composition.
31917232	10	4	theme	presenting	1382:1391	arg1	pores					1393:1397	the tissue presenting pores	1371:1397	the tissue presenting pores aligned with the fibers	1371:1421	This was likely due to the unique structural organization of the tissue presenting pores aligned with the fibers, as observed in SEM images.
31917232	1	5	theme	meniscus	157:164	arg1	pathophysiology					134:148	the pathophysiology	130:148	the pathophysiology of the meniscus	130:164	OBJECTIVE To date, the pathophysiology of the meniscus has not been fully elucidated.
31917232	13	6	from	transport	1723:1731	arg1	tissue					1745:1750	meniscal tissue	1736:1750	meniscal tissue	1736:1750	CONCLUSIONS This study provides new knowledge on the mechanisms of molecular transport in meniscal tissue.
31917232	5	7	theme	structure	736:744	arg1	role					704:707	the role	700:707	the role of solute size, and tissue structure and composition on molecular diffusion in meniscus tissue	700:802	The objective of this study was to investigate the role of solute size, and tissue structure and composition on molecular diffusion in meniscus tissue.
31917232	0	8	from	Molecular	0:8	arg1	meniscus					48:55	human meniscus	42:55	human meniscus	42:55	Molecular and macromolecular diffusion in human meniscus: relationships with tissue structure and composition.
31917232	8	9	theme	SEM	1069:1071	arg1	images					1073:1078	SEM images	1069:1078	SEM images	1069:1078	SEM images were used to investigate collagen structure in relation to transport mechanisms.
31917232	6	10	theme	FRAP	827:830	arg1	technique					832:840	a custom FRAP technique	818:840	a custom FRAP technique developed in our lab	818:861	DESIGN Using a custom FRAP technique developed in our lab, we measured the direction-dependent diffusivity in human meniscus of six different molecular probes of size ranging from ∼300Da to 150,000Da.
31917232	12	11	theme	diffusion	1590:1598	arg1	coefficients					1600:1611	diffusion coefficients	1590:1611	diffusion coefficients	1590:1611	No significant correlations were found among diffusion coefficients and water content of the tissue.
31917232	11	12	theme	Diffusion	1451:1459	arg1	coefficients					1461:1472	Diffusion coefficients	1451:1472	Diffusion coefficients	1451:1472	Diffusion coefficients decreased as the molecular size increased, following the Ogston model.
31917232	6	13	theme	human	915:919	arg1	meniscus					921:928	human meniscus	915:928	human meniscus of six different molecular probes of size ranging from ∼300Da to 150,000Da	915:1003	DESIGN Using a custom FRAP technique developed in our lab, we measured the direction-dependent diffusivity in human meniscus of six different molecular probes of size ranging from ∼300Da to 150,000Da.
31917232	9	14	theme	RESULTS	1161:1167	arg1	Diffusivity					1169:1179	RESULTS Diffusivity	1161:1179	RESULTS Diffusivity	1161:1179	RESULTS Diffusivity was anisotropic, being significantly faster in the direction parallel to collagen fibers when compared the orthogonal direction.
31917232	6	15	theme	custom	820:825	arg1	technique					832:840	a custom FRAP technique	818:840	a custom FRAP technique developed in our lab	818:861	DESIGN Using a custom FRAP technique developed in our lab, we measured the direction-dependent diffusivity in human meniscus of six different molecular probes of size ranging from ∼300Da to 150,000Da.
31917232	11	16	theme	molecular	1491:1499	arg1	size					1501:1504	the molecular size	1487:1504	the molecular size	1487:1504	Diffusion coefficients decreased as the molecular size increased, following the Ogston model.
31917232	5	17	theme	composition	750:760	arg1	role					704:707	the role	700:707	the role of solute size, and tissue structure and composition on molecular diffusion in meniscus tissue	700:802	The objective of this study was to investigate the role of solute size, and tissue structure and composition on molecular diffusion in meniscus tissue.
31917232	8	18	used	used	1085:1088	arg2	images					1073:1078	SEM images	1069:1078	SEM images	1069:1078	SEM images were used to investigate collagen structure in relation to transport mechanisms.
31917232	0	19	with	relationships	58:70	arg1	composition					98:108	composition	98:108	composition	98:108	Molecular and macromolecular diffusion in human meniscus: relationships with tissue structure and composition.
31917232	0	19	with	relationships	58:70	arg1	structure					84:92	tissue structure	77:92	tissue structure	77:92	Molecular and macromolecular diffusion in human meniscus: relationships with tissue structure and composition.
31917232	2	20	theme	molecular	262:270	arg1	signals					272:278	other molecular signals	256:278	other molecular signals	256:278	Due to the tissue's limited vascularization, nutrients and other molecular signals spread through the extracellular matrix via diffusion or convection (interstitial fluid flow).
31917232	12	21	theme	tissue	1638:1643	arg1	coefficients					1600:1611	diffusion coefficients	1590:1611	diffusion coefficients	1590:1611	No significant correlations were found among diffusion coefficients and water content of the tissue.
31917232	12	21	theme	tissue	1638:1643	arg1	content					1623:1629	water content	1617:1629	water content of the tissue	1617:1643	No significant correlations were found among diffusion coefficients and water content of the tissue.
31917232	13	22	theme	meniscal	1736:1743	arg1	tissue					1745:1750	meniscal tissue	1736:1750	meniscal tissue	1736:1750	CONCLUSIONS This study provides new knowledge on the mechanisms of molecular transport in meniscal tissue.
31917232	9	23	theme	parallel	1242:1249	arg1	direction					1232:1240	the direction	1228:1240	the direction parallel to collagen fibers	1228:1268	RESULTS Diffusivity was anisotropic, being significantly faster in the direction parallel to collagen fibers when compared the orthogonal direction.
31917232	2	24	theme	other	256:260	arg1	signals					272:278	other molecular signals	256:278	other molecular signals	256:278	Due to the tissue's limited vascularization, nutrients and other molecular signals spread through the extracellular matrix via diffusion or convection (interstitial fluid flow).
31917232	6	25	theme	probes	957:962	arg1	meniscus					921:928	human meniscus	915:928	human meniscus of six different molecular probes of size ranging from ∼300Da to 150,000Da	915:1003	DESIGN Using a custom FRAP technique developed in our lab, we measured the direction-dependent diffusivity in human meniscus of six different molecular probes of size ranging from ∼300Da to 150,000Da.
31917232	5	26	theme	meniscus	788:795	arg1	tissue					797:802	meniscus tissue	788:802	meniscus tissue	788:802	The objective of this study was to investigate the role of solute size, and tissue structure and composition on molecular diffusion in meniscus tissue.
31917232	13	27	from	knowledge	1682:1690	arg1	mechanisms					1699:1708	the mechanisms	1695:1708	the mechanisms of molecular transport in meniscal tissue	1695:1750	CONCLUSIONS This study provides new knowledge on the mechanisms of molecular transport in meniscal tissue.
31917232	13	28	from	tissue	1745:1750	arg1	mechanisms					1699:1708	the mechanisms	1695:1708	the mechanisms of molecular transport in meniscal tissue	1695:1750	CONCLUSIONS This study provides new knowledge on the mechanisms of molecular transport in meniscal tissue.
31917232	8	29	theme	collagen	1105:1112	arg1	structure					1114:1122	collagen structure	1105:1122	collagen structure	1105:1122	SEM images were used to investigate collagen structure in relation to transport mechanisms.
31917232	3	30	theme	transport	389:397	arg1	mechanisms					399:408	transport mechanisms	389:408	transport mechanisms	389:408	Understanding transport mechanisms is crucial to elucidating meniscal pathophysiology, and to designing treatments for repair and restoration of the tissue.
31917232	6	31	from	diffusivity	900:910	arg1	meniscus					921:928	human meniscus	915:928	human meniscus of six different molecular probes of size ranging from ∼300Da to 150,000Da	915:1003	DESIGN Using a custom FRAP technique developed in our lab, we measured the direction-dependent diffusivity in human meniscus of six different molecular probes of size ranging from ∼300Da to 150,000Da.
31917232	9	32	theme	collagen	1254:1261	arg1	fibers					1263:1268	collagen fibers	1254:1268	collagen fibers	1254:1268	RESULTS Diffusivity was anisotropic, being significantly faster in the direction parallel to collagen fibers when compared the orthogonal direction.
31917232	2	33	theme	extracellular	299:311	arg1	matrix					313:318	the extracellular matrix	295:318	the extracellular matrix	295:318	Due to the tissue's limited vascularization, nutrients and other molecular signals spread through the extracellular matrix via diffusion or convection (interstitial fluid flow).
31917232	14	34	dep	tissue	1866:1871	arg1	restoration					1873:1883	restoration	1873:1883	restoration	1873:1883	The reported results can be leveraged to further investigate tissue pathophysiology and to design treatments for tissue restoration or replacement.
31917232	3	35	theme	meniscal	436:443	arg1	pathophysiology					445:459	meniscal pathophysiology	436:459	meniscal pathophysiology	436:459	Understanding transport mechanisms is crucial to elucidating meniscal pathophysiology, and to designing treatments for repair and restoration of the tissue.
31917232	5	36	theme	size	719:722	arg1	role					704:707	the role	700:707	the role of solute size, and tissue structure and composition on molecular diffusion in meniscus tissue	700:802	The objective of this study was to investigate the role of solute size, and tissue structure and composition on molecular diffusion in meniscus tissue.
31917232	10	37	theme	SEM	1439:1441	arg1	images					1443:1448	SEM images	1439:1448	SEM images	1439:1448	This was likely due to the unique structural organization of the tissue presenting pores aligned with the fibers, as observed in SEM images.
31917232	3	38	theme	designing	469:477	arg1	treatments					479:488	designing treatments	469:488	designing treatments for repair and restoration of the tissue	469:529	Understanding transport mechanisms is crucial to elucidating meniscal pathophysiology, and to designing treatments for repair and restoration of the tissue.
31917232	0	39	theme	macromolecular	14:27	arg1	diffusion					29:37	macromolecular diffusion	14:37	macromolecular diffusion	14:37	Molecular and macromolecular diffusion in human meniscus: relationships with tissue structure and composition.
31917232	12	40	theme	significant	1548:1558	arg1	correlations					1560:1571	No significant correlations	1545:1571	No significant correlations	1545:1571	No significant correlations were found among diffusion coefficients and water content of the tissue.
31917232	10	41	theme	structural	1344:1353	arg1	organization					1355:1366	the unique structural organization	1333:1366	the unique structural organization of the tissue presenting pores aligned with the fibers	1333:1421	This was likely due to the unique structural organization of the tissue presenting pores aligned with the fibers, as observed in SEM images.
31917232	0	42	theme	human	42:46	arg1	meniscus					48:55	human meniscus	42:55	human meniscus	42:55	Molecular and macromolecular diffusion in human meniscus: relationships with tissue structure and composition.
31917232	13	43	theme	molecular	1713:1721	arg1	transport					1723:1731	molecular transport	1713:1731	molecular transport in meniscal tissue	1713:1750	CONCLUSIONS This study provides new knowledge on the mechanisms of molecular transport in meniscal tissue.
31917232	2	44	theme	limited	217:223	arg1	vascularization					225:239	the tissue's limited vascularization	204:239	the tissue's limited vascularization	204:239	Due to the tissue's limited vascularization, nutrients and other molecular signals spread through the extracellular matrix via diffusion or convection (interstitial fluid flow).
31917232	10	45	theme	unique	1337:1342	arg1	organization					1355:1366	the unique structural organization	1333:1366	the unique structural organization of the tissue presenting pores aligned with the fibers	1333:1421	This was likely due to the unique structural organization of the tissue presenting pores aligned with the fibers, as observed in SEM images.
31917232	6	46	theme	size	967:970	arg1	probes					957:962	six different molecular probes	933:962	six different molecular probes of size ranging from ∼300Da to 150,000Da	933:1003	DESIGN Using a custom FRAP technique developed in our lab, we measured the direction-dependent diffusivity in human meniscus of six different molecular probes of size ranging from ∼300Da to 150,000Da.
31917232	5	47	theme	tissue	729:734	arg1	structure					736:744	tissue structure	729:744	tissue structure	729:744	The objective of this study was to investigate the role of solute size, and tissue structure and composition on molecular diffusion in meniscus tissue.
31917232	14	48	theme	reported	1757:1764	arg1	results					1766:1772	The reported results	1753:1772	The reported results	1753:1772	The reported results can be leveraged to further investigate tissue pathophysiology and to design treatments for tissue restoration or replacement.
31917232	12	49	theme	water	1617:1621	arg1	content					1623:1629	water content	1617:1629	water content of the tissue	1617:1643	No significant correlations were found among diffusion coefficients and water content of the tissue.
31917232	10	50	theme	pores	1393:1397	arg1	organization					1355:1366	the unique structural organization	1333:1366	the unique structural organization of the tissue presenting pores aligned with the fibers	1333:1421	This was likely due to the unique structural organization of the tissue presenting pores aligned with the fibers, as observed in SEM images.
31917232	6	51	theme	molecular	947:955	arg1	probes					957:962	six different molecular probes	933:962	six different molecular probes of size ranging from ∼300Da to 150,000Da	933:1003	DESIGN Using a custom FRAP technique developed in our lab, we measured the direction-dependent diffusivity in human meniscus of six different molecular probes of size ranging from ∼300Da to 150,000Da.
31917232	4	52	theme	Similar	532:538	arg1	morphology					589:598	Similar to other fibrocartilaginous structures, meniscal morphology	532:598	Similar to other fibrocartilaginous structures, meniscal morphology	532:598	Similar to other fibrocartilaginous structures, meniscal morphology and composition may affect its diffusive properties.
31917232	13	53	theme	new	1678:1680	arg1	knowledge					1682:1690	new knowledge	1678:1690	new knowledge on the mechanisms of molecular transport in meniscal tissue	1678:1750	CONCLUSIONS This study provides new knowledge on the mechanisms of molecular transport in meniscal tissue.
31917232	9	54	theme	orthogonal	1288:1297	arg1	direction					1299:1307	the orthogonal direction	1284:1307	the orthogonal direction	1284:1307	RESULTS Diffusivity was anisotropic, being significantly faster in the direction parallel to collagen fibers when compared the orthogonal direction.
31917232	7	55	theme	sample	1047:1052	arg1	content					1060:1066	sample water content	1047:1066	sample water content	1047:1066	Diffusivity measurements were related to sample water content.
31917232	4	56	dep	Similar	532:538	arg1	meniscal					580:587	meniscal	580:587	meniscal	580:587	Similar to other fibrocartilaginous structures, meniscal morphology and composition may affect its diffusive properties.
31917232	4	57	theme	fibrocartilaginous	549:566	arg1	structures					568:577	other fibrocartilaginous structures	543:577	other fibrocartilaginous structures	543:577	Similar to other fibrocartilaginous structures, meniscal morphology and composition may affect its diffusive properties.
31917232	6	58	theme	different	937:945	arg1	probes					957:962	six different molecular probes	933:962	six different molecular probes of size ranging from ∼300Da to 150,000Da	933:1003	DESIGN Using a custom FRAP technique developed in our lab, we measured the direction-dependent diffusivity in human meniscus of six different molecular probes of size ranging from ∼300Da to 150,000Da.
31917232	10	59	theme	tissue	1375:1380	arg1	pores					1393:1397	the tissue presenting pores	1371:1397	the tissue presenting pores aligned with the fibers	1371:1421	This was likely due to the unique structural organization of the tissue presenting pores aligned with the fibers, as observed in SEM images.
31917232	11	60	theme	Ogston	1531:1536	arg1	model					1538:1542	the Ogston model	1527:1542	the Ogston model	1527:1542	Diffusion coefficients decreased as the molecular size increased, following the Ogston model.
31917232	4	61	theme	other	543:547	arg1	structures					568:577	other fibrocartilaginous structures	543:577	other fibrocartilaginous structures	543:577	Similar to other fibrocartilaginous structures, meniscal morphology and composition may affect its diffusive properties.
31917232	7	62	theme	water	1054:1058	arg1	content					1060:1066	sample water content	1047:1066	sample water content	1047:1066	Diffusivity measurements were related to sample water content.
31917232	8	63	theme	transport	1139:1147	arg1	mechanisms					1149:1158	transport mechanisms	1139:1158	transport mechanisms	1139:1158	SEM images were used to investigate collagen structure in relation to transport mechanisms.
31917232	13	64	dep	CONCLUSIONS	1646:1656	arg1	provides					1669:1676	provides	1669:1676	provides new knowledge on the mechanisms of molecular transport in meniscal tissue	1669:1750	CONCLUSIONS This study provides new knowledge on the mechanisms of molecular transport in meniscal tissue.
31917232	13	65	theme	transport	1723:1731	arg1	mechanisms					1699:1708	the mechanisms	1695:1708	the mechanisms of molecular transport in meniscal tissue	1695:1750	CONCLUSIONS This study provides new knowledge on the mechanisms of molecular transport in meniscal tissue.
31917232	4	66	theme	diffusive	631:639	arg1	properties					641:650	its diffusive properties	627:650	its diffusive properties	627:650	Similar to other fibrocartilaginous structures, meniscal morphology and composition may affect its diffusive properties.
31917232	5	67	theme	solute	712:717	arg1	size					719:722	solute size	712:722	solute size	712:722	The objective of this study was to investigate the role of solute size, and tissue structure and composition on molecular diffusion in meniscus tissue.
31917232	7	68	theme	Diffusivity	1006:1016	arg1	measurements					1018:1029	Diffusivity measurements	1006:1029	Diffusivity measurements	1006:1029	Diffusivity measurements were related to sample water content.
31917232	5	69	theme	molecular	765:773	arg1	diffusion					775:783	molecular diffusion	765:783	molecular diffusion in meniscus tissue	765:802	The objective of this study was to investigate the role of solute size, and tissue structure and composition on molecular diffusion in meniscus tissue.
31917232	5	70	from	diffusion	775:783	arg1	tissue					797:802	meniscus tissue	788:802	meniscus tissue	788:802	The objective of this study was to investigate the role of solute size, and tissue structure and composition on molecular diffusion in meniscus tissue.
31917232	3	71	theme	tissue	524:529	arg1	restoration					505:515	restoration	505:515	restoration	505:515	Understanding transport mechanisms is crucial to elucidating meniscal pathophysiology, and to designing treatments for repair and restoration of the tissue.
31917232	3	71	theme	tissue	524:529	arg1	repair					494:499	repair	494:499	repair	494:499	Understanding transport mechanisms is crucial to elucidating meniscal pathophysiology, and to designing treatments for repair and restoration of the tissue.
31917232	14	72	theme	tissue	1814:1819	arg1	pathophysiology					1821:1835	tissue pathophysiology	1814:1835	tissue pathophysiology	1814:1835	The reported results can be leveraged to further investigate tissue pathophysiology and to design treatments for tissue restoration or replacement.
31917232	13	73	from	mechanisms	1699:1708	arg1	tissue					1745:1750	meniscal tissue	1736:1750	meniscal tissue	1736:1750	CONCLUSIONS This study provides new knowledge on the mechanisms of molecular transport in meniscal tissue.
31917232	0	74	from	diffusion	29:37	arg1	meniscus					48:55	human meniscus	42:55	human meniscus	42:55	Molecular and macromolecular diffusion in human meniscus: relationships with tissue structure and composition.
31917232	0	75	dep	Molecular	0:8	arg1	relationships					58:70	relationships	58:70	Molecular and macromolecular diffusion in human meniscus: relationships with tissue structure and composition.	0:109	Molecular and macromolecular diffusion in human meniscus: relationships with tissue structure and composition.
31917232	2	76	theme	fluid	362:366	arg1	diffusion					324:332	diffusion	324:332	diffusion	324:332	Due to the tissue's limited vascularization, nutrients and other molecular signals spread through the extracellular matrix via diffusion or convection (interstitial fluid flow).
31917232	2	76	theme	fluid	362:366	arg1	flow					368:371	interstitial fluid flow	349:371	interstitial fluid flow	349:371	Due to the tissue's limited vascularization, nutrients and other molecular signals spread through the extracellular matrix via diffusion or convection (interstitial fluid flow).
31917232	5	77	from	role	704:707	arg1	diffusion					775:783	molecular diffusion	765:783	molecular diffusion in meniscus tissue	765:802	The objective of this study was to investigate the role of solute size, and tissue structure and composition on molecular diffusion in meniscus tissue.
32562783	7	0	theme	OPN	1387:1389	arg1	expression					1402:1411	ALP, OCN, OPN and RUNX-2 expression	1377:1411	expression	1402:1411	Results of qRT-PCR and Western blot indicated that the high dose of HYSA/scaffolds can up-regulate ALP, OCN, OPN and RUNX-2 expression and relative protein secretion of the HIF-1α and BMP-2.
32562783	8	1	theme	bone	1578:1581	arg1	formation					1583:1591	new bone formation	1574:1591	new bone formation	1574:1591	In the animal experiment, the high dose of HYSA/scaffolds has a significantly better capacity to promote new bone formation than the undoped scaffolds at 8 weeks post-surgery.
32562783	5	2	theme	vein	1140:1143	arg1	HUVECs					1164:1169	HUVECs	1164:1169	HUVECs	1164:1169	In vitro the cell culture results exhibited that the high dose of HYSA (0.5 mg/ml) loaded scaffolds can promote the proliferation of bone marrow stromal cells (rBMSCs) and migration, tubule formation of human umbilical vein endothelial cells (HUVECs).
32562783	5	2	theme	vein	1140:1143	arg1	cells					1157:1161	human umbilical vein endothelial cells	1124:1161	human umbilical vein endothelial cells (HUVECs)	1124:1170	In vitro the cell culture results exhibited that the high dose of HYSA (0.5 mg/ml) loaded scaffolds can promote the proliferation of bone marrow stromal cells (rBMSCs) and migration, tubule formation of human umbilical vein endothelial cells (HUVECs).
32562783	7	3	theme	OCN	1382:1384	arg1	expression					1402:1411	ALP, OCN, OPN and RUNX-2 expression	1377:1411	expression	1402:1411	Results of qRT-PCR and Western blot indicated that the high dose of HYSA/scaffolds can up-regulate ALP, OCN, OPN and RUNX-2 expression and relative protein secretion of the HIF-1α and BMP-2.
32562783	2	4	theme	print	528:532	arg1	technique					534:542	a 3D print technique	523:542	a 3D print technique	523:542	Herein, we hypothetically speculated that the synthetic bioactive glasses (BG, 1393) scaffolds carried HYSA by a 3D print technique could enhance osteogenic repair properties.
32562783	9	5	theme	bone	1778:1781	arg1	biomaterials					1802:1813	effective and safe bone tissue engineering biomaterials	1759:1813	effective and safe bone tissue engineering biomaterials for bone regeneration	1759:1835	Thus, our results claimed that the novel HYSA/scaffolds hold the substantial potential to be further developed as effective and safe bone tissue engineering biomaterials for bone regeneration by combining enhanced osteogenesis and angiogenesis.
32562783	5	6	theme	cells	1157:1161	arg1	migration					1093:1101	migration	1093:1101	migration	1093:1101	In vitro the cell culture results exhibited that the high dose of HYSA (0.5 mg/ml) loaded scaffolds can promote the proliferation of bone marrow stromal cells (rBMSCs) and migration, tubule formation of human umbilical vein endothelial cells (HUVECs).
32562783	5	6	theme	cells	1157:1161	arg1	formation					1111:1119	tubule formation	1104:1119	tubule formation of human umbilical vein endothelial cells (HUVECs)	1104:1170	In vitro the cell culture results exhibited that the high dose of HYSA (0.5 mg/ml) loaded scaffolds can promote the proliferation of bone marrow stromal cells (rBMSCs) and migration, tubule formation of human umbilical vein endothelial cells (HUVECs).
32562783	5	6	theme	cells	1157:1161	arg1	proliferation					1037:1049	the proliferation	1033:1049	the proliferation of bone marrow stromal cells (rBMSCs)	1033:1087	In vitro the cell culture results exhibited that the high dose of HYSA (0.5 mg/ml) loaded scaffolds can promote the proliferation of bone marrow stromal cells (rBMSCs) and migration, tubule formation of human umbilical vein endothelial cells (HUVECs).
32562783	7	7	theme	ALP	1377:1379	arg1	expression					1402:1411	ALP, OCN, OPN and RUNX-2 expression	1377:1411	expression	1402:1411	Results of qRT-PCR and Western blot indicated that the high dose of HYSA/scaffolds can up-regulate ALP, OCN, OPN and RUNX-2 expression and relative protein secretion of the HIF-1α and BMP-2.
32562783	9	8	theme	engineering	1790:1800	arg1	biomaterials					1802:1813	effective and safe bone tissue engineering biomaterials	1759:1813	effective and safe bone tissue engineering biomaterials for bone regeneration	1759:1835	Thus, our results claimed that the novel HYSA/scaffolds hold the substantial potential to be further developed as effective and safe bone tissue engineering biomaterials for bone regeneration by combining enhanced osteogenesis and angiogenesis.
32562783	6	9	theme	alkaline	1184:1191	arg1	ALP					1206:1208	ALP	1206:1208	ALP	1206:1208	The active alkaline phosphatase (ALP) of rBMSCs can also be improved by the high dose of HYSA/scaffolds.
32562783	6	9	theme	alkaline	1184:1191	arg1	phosphatase					1193:1203	The active alkaline phosphatase	1173:1203	The active alkaline phosphatase (ALP) of rBMSCs	1173:1219	The active alkaline phosphatase (ALP) of rBMSCs can also be improved by the high dose of HYSA/scaffolds.
32562783	8	10	theme	undoped	1602:1608	arg1	scaffolds					1610:1618	the undoped scaffolds	1598:1618	the undoped scaffolds at 8 weeks post-surgery	1598:1642	In the animal experiment, the high dose of HYSA/scaffolds has a significantly better capacity to promote new bone formation than the undoped scaffolds at 8 weeks post-surgery.
32562783	2	11	theme	osteogenic	558:567	arg1	properties					576:585	osteogenic repair properties	558:585	osteogenic repair properties	558:585	Herein, we hypothetically speculated that the synthetic bioactive glasses (BG, 1393) scaffolds carried HYSA by a 3D print technique could enhance osteogenic repair properties.
32562783	7	12	theme	Western	1301:1307	arg1	blot					1309:1312	Western blot	1301:1312	Western blot	1301:1312	Results of qRT-PCR and Western blot indicated that the high dose of HYSA/scaffolds can up-regulate ALP, OCN, OPN and RUNX-2 expression and relative protein secretion of the HIF-1α and BMP-2.
32562783	0	13	theme	bone	110:113	arg1	repair					115:120	bone repair	110:120	bone repair	110:120	A novel 3D printed bioactive scaffolds with enhanced osteogenic inspired by ancient Chinese medicine HYSA for bone repair.
32562783	5	14	theme	scaffolds	1011:1019	arg1	dose					979:982	the high dose	970:982	the high dose of HYSA (0.5 mg/ml) loaded scaffolds	970:1019	In vitro the cell culture results exhibited that the high dose of HYSA (0.5 mg/ml) loaded scaffolds can promote the proliferation of bone marrow stromal cells (rBMSCs) and migration, tubule formation of human umbilical vein endothelial cells (HUVECs).
32562783	7	15	theme	qRT-PCR	1289:1295	arg1	Results					1278:1284	Results	1278:1284	Results of qRT-PCR and Western blot	1278:1312	Results of qRT-PCR and Western blot indicated that the high dose of HYSA/scaffolds can up-regulate ALP, OCN, OPN and RUNX-2 expression and relative protein secretion of the HIF-1α and BMP-2.
32562783	5	16	dep	results	947:953	arg1	exhibited					955:963	exhibited	955:963	results exhibited that the high dose of HYSA (0.5 mg/ml) loaded scaffolds can promote the proliferation of bone marrow stromal cells (rBMSCs) and migration, tubule formation of human umbilical vein endothelial cells (HUVECs)	947:1170	In vitro the cell culture results exhibited that the high dose of HYSA (0.5 mg/ml) loaded scaffolds can promote the proliferation of bone marrow stromal cells (rBMSCs) and migration, tubule formation of human umbilical vein endothelial cells (HUVECs).
32562783	3	17	theme	mechanical	727:736	arg1	strength					738:745	the BG mechanical strength	720:745	the BG mechanical strength	720:745	Notably, scaffolds coating chitosan/sodium alginate endowed with excellent drug control release ability, and significantly improved the BG mechanical strength.
32562783	1	18	theme	orthopedics	348:358	arg1	field					360:364	orthopedics field	348:364	orthopedics field	348:364	Some traditional Chinese medicine (TCM) has been applied in bone repair, however, hydroxy-safflower yellow A (HYSA), one composition of safflower of the typical invigorating the circulation of TCM, has little been studied in orthopedics field for osteogenesis and angiogenesis clinically.
32562783	6	19	theme	rBMSCs	1214:1219	arg1	ALP					1206:1208	ALP	1206:1208	ALP	1206:1208	The active alkaline phosphatase (ALP) of rBMSCs can also be improved by the high dose of HYSA/scaffolds.
32562783	6	19	theme	rBMSCs	1214:1219	arg1	phosphatase					1193:1203	The active alkaline phosphatase	1173:1203	The active alkaline phosphatase (ALP) of rBMSCs	1173:1219	The active alkaline phosphatase (ALP) of rBMSCs can also be improved by the high dose of HYSA/scaffolds.
32562783	2	20	theme	synthetic	458:466	arg1	glasses					478:484	synthetic bioactive glasses	458:484	the synthetic bioactive glasses (BG, 1393) scaffolds carried HYSA by a 3D print technique	454:542	Herein, we hypothetically speculated that the synthetic bioactive glasses (BG, 1393) scaffolds carried HYSA by a 3D print technique could enhance osteogenic repair properties.
32562783	5	21	theme	stromal	1066:1072	arg1	cells					1074:1078	bone marrow stromal cells	1054:1078	bone marrow stromal cells (rBMSCs)	1054:1087	In vitro the cell culture results exhibited that the high dose of HYSA (0.5 mg/ml) loaded scaffolds can promote the proliferation of bone marrow stromal cells (rBMSCs) and migration, tubule formation of human umbilical vein endothelial cells (HUVECs).
32562783	5	21	theme	stromal	1066:1072	arg1	rBMSCs					1081:1086	rBMSCs	1081:1086	rBMSCs	1081:1086	In vitro the cell culture results exhibited that the high dose of HYSA (0.5 mg/ml) loaded scaffolds can promote the proliferation of bone marrow stromal cells (rBMSCs) and migration, tubule formation of human umbilical vein endothelial cells (HUVECs).
32562783	1	22	theme	Chinese	140:146	arg1	TCM					158:160	TCM	158:160	TCM	158:160	Some traditional Chinese medicine (TCM) has been applied in bone repair, however, hydroxy-safflower yellow A (HYSA), one composition of safflower of the typical invigorating the circulation of TCM, has little been studied in orthopedics field for osteogenesis and angiogenesis clinically.
32562783	1	22	theme	Chinese	140:146	arg1	medicine					148:155	Some traditional Chinese medicine	123:155	Some traditional Chinese medicine (TCM)	123:161	Some traditional Chinese medicine (TCM) has been applied in bone repair, however, hydroxy-safflower yellow A (HYSA), one composition of safflower of the typical invigorating the circulation of TCM, has little been studied in orthopedics field for osteogenesis and angiogenesis clinically.
32562783	7	23	theme	BMP-2	1462:1466	arg1	expression					1402:1411	ALP, OCN, OPN and RUNX-2 expression	1377:1411	expression	1402:1411	Results of qRT-PCR and Western blot indicated that the high dose of HYSA/scaffolds can up-regulate ALP, OCN, OPN and RUNX-2 expression and relative protein secretion of the HIF-1α and BMP-2.
32562783	7	23	theme	BMP-2	1462:1466	arg1	secretion					1434:1442	relative protein secretion	1417:1442	relative protein secretion of the HIF-1α and BMP-2	1417:1466	Results of qRT-PCR and Western blot indicated that the high dose of HYSA/scaffolds can up-regulate ALP, OCN, OPN and RUNX-2 expression and relative protein secretion of the HIF-1α and BMP-2.
32562783	8	24	contain	has	1527:1529	arg2	capacity					1554:1561	a significantly better capacity	1531:1561	a significantly better capacity to promote new bone formation than the undoped scaffolds at 8 weeks post-surgery	1531:1642	In the animal experiment, the high dose of HYSA/scaffolds has a significantly better capacity to promote new bone formation than the undoped scaffolds at 8 weeks post-surgery.
32562783	8	24	contain	has	1527:1529	arg1	dose					1504:1507	the high dose	1495:1507	the high dose of HYSA/scaffolds	1495:1525	In the animal experiment, the high dose of HYSA/scaffolds has a significantly better capacity to promote new bone formation than the undoped scaffolds at 8 weeks post-surgery.
32562783	0	25	theme	Chinese	84:90	arg1	medicine					92:99	ancient Chinese medicine	76:99	ancient Chinese medicine HYSA for bone repair	76:120	A novel 3D printed bioactive scaffolds with enhanced osteogenic inspired by ancient Chinese medicine HYSA for bone repair.
32562783	7	26	theme	HIF-1α	1451:1456	arg1	expression					1402:1411	ALP, OCN, OPN and RUNX-2 expression	1377:1411	expression	1402:1411	Results of qRT-PCR and Western blot indicated that the high dose of HYSA/scaffolds can up-regulate ALP, OCN, OPN and RUNX-2 expression and relative protein secretion of the HIF-1α and BMP-2.
32562783	7	26	theme	HIF-1α	1451:1456	arg1	secretion					1434:1442	relative protein secretion	1417:1442	relative protein secretion of the HIF-1α and BMP-2	1417:1466	Results of qRT-PCR and Western blot indicated that the high dose of HYSA/scaffolds can up-regulate ALP, OCN, OPN and RUNX-2 expression and relative protein secretion of the HIF-1α and BMP-2.
32562783	2	27	theme	glasses	478:484	arg1	scaffolds					497:505	the synthetic bioactive glasses (BG, 1393) scaffolds	454:505	the synthetic bioactive glasses (BG, 1393) scaffolds carried HYSA by a 3D print technique	454:542	Herein, we hypothetically speculated that the synthetic bioactive glasses (BG, 1393) scaffolds carried HYSA by a 3D print technique could enhance osteogenic repair properties.
32562783	5	28	theme	bone	1054:1057	arg1	cells					1074:1078	bone marrow stromal cells	1054:1078	bone marrow stromal cells (rBMSCs)	1054:1087	In vitro the cell culture results exhibited that the high dose of HYSA (0.5 mg/ml) loaded scaffolds can promote the proliferation of bone marrow stromal cells (rBMSCs) and migration, tubule formation of human umbilical vein endothelial cells (HUVECs).
32562783	5	28	theme	bone	1054:1057	arg1	rBMSCs					1081:1086	rBMSCs	1081:1086	rBMSCs	1081:1086	In vitro the cell culture results exhibited that the high dose of HYSA (0.5 mg/ml) loaded scaffolds can promote the proliferation of bone marrow stromal cells (rBMSCs) and migration, tubule formation of human umbilical vein endothelial cells (HUVECs).
32562783	4	29	theme	alginate	809:816	arg1	film					818:821	chitosan/sodium alginate film	793:821	chitosan/sodium alginate film	793:821	HYSA was loaded into BG scaffolds by coating chitosan/sodium alginate film, and the osteogenesis and angiogenesis of the HYSA/scaffolds were evaluated in vitro and in vivo.
32562783	5	30	theme	tubule	1104:1109	arg1	formation					1111:1119	tubule formation	1104:1119	tubule formation of human umbilical vein endothelial cells (HUVECs)	1104:1170	In vitro the cell culture results exhibited that the high dose of HYSA (0.5 mg/ml) loaded scaffolds can promote the proliferation of bone marrow stromal cells (rBMSCs) and migration, tubule formation of human umbilical vein endothelial cells (HUVECs).
32562783	0	31	theme	3D	8:9	arg1	scaffolds					29:37	A novel 3D printed bioactive scaffolds	0:37	A novel 3D printed bioactive scaffolds with enhanced osteogenic	0:62	A novel 3D printed bioactive scaffolds with enhanced osteogenic inspired by ancient Chinese medicine HYSA for bone repair.
32562783	0	32	theme	bioactive	19:27	arg1	scaffolds					29:37	A novel 3D printed bioactive scaffolds	0:37	A novel 3D printed bioactive scaffolds with enhanced osteogenic	0:62	A novel 3D printed bioactive scaffolds with enhanced osteogenic inspired by ancient Chinese medicine HYSA for bone repair.
32562783	7	33	theme	relative	1417:1424	arg1	secretion					1434:1442	relative protein secretion	1417:1442	relative protein secretion of the HIF-1α and BMP-2	1417:1466	Results of qRT-PCR and Western blot indicated that the high dose of HYSA/scaffolds can up-regulate ALP, OCN, OPN and RUNX-2 expression and relative protein secretion of the HIF-1α and BMP-2.
32562783	4	34	theme	chitosan/sodium	793:807	arg1	film					818:821	chitosan/sodium alginate film	793:821	chitosan/sodium alginate film	793:821	HYSA was loaded into BG scaffolds by coating chitosan/sodium alginate film, and the osteogenesis and angiogenesis of the HYSA/scaffolds were evaluated in vitro and in vivo.
32562783	1	35	theme	typical	276:282	arg1	circulation					301:311	the typical invigorating the circulation	272:311	the typical invigorating the circulation of TCM	272:318	Some traditional Chinese medicine (TCM) has been applied in bone repair, however, hydroxy-safflower yellow A (HYSA), one composition of safflower of the typical invigorating the circulation of TCM, has little been studied in orthopedics field for osteogenesis and angiogenesis clinically.
32562783	5	36	theme	HYSA	987:990	arg1	scaffolds					1011:1019	HYSA (0.5 mg/ml) loaded scaffolds	987:1019	HYSA (0.5 mg/ml) loaded scaffolds	987:1019	In vitro the cell culture results exhibited that the high dose of HYSA (0.5 mg/ml) loaded scaffolds can promote the proliferation of bone marrow stromal cells (rBMSCs) and migration, tubule formation of human umbilical vein endothelial cells (HUVECs).
32562783	3	37	theme	endowed	640:646	arg1	alginate					631:638	chitosan/sodium alginate	615:638	chitosan/sodium alginate endowed with excellent drug control	615:674	Notably, scaffolds coating chitosan/sodium alginate endowed with excellent drug control release ability, and significantly improved the BG mechanical strength.
32562783	8	38	from	weeks	1625:1629	arg1	scaffolds					1610:1618	the undoped scaffolds	1598:1618	the undoped scaffolds at 8 weeks post-surgery	1598:1642	In the animal experiment, the high dose of HYSA/scaffolds has a significantly better capacity to promote new bone formation than the undoped scaffolds at 8 weeks post-surgery.
32562783	3	39	theme	chitosan/sodium	615:629	arg1	alginate					631:638	chitosan/sodium alginate	615:638	chitosan/sodium alginate endowed with excellent drug control	615:674	Notably, scaffolds coating chitosan/sodium alginate endowed with excellent drug control release ability, and significantly improved the BG mechanical strength.
32562783	4	40	theme	BG	769:770	arg1	scaffolds					772:780	BG scaffolds	769:780	BG scaffolds	769:780	HYSA was loaded into BG scaffolds by coating chitosan/sodium alginate film, and the osteogenesis and angiogenesis of the HYSA/scaffolds were evaluated in vitro and in vivo.
32562783	8	41	theme	better	1547:1552	arg1	capacity					1554:1561	a significantly better capacity	1531:1561	a significantly better capacity to promote new bone formation than the undoped scaffolds at 8 weeks post-surgery	1531:1642	In the animal experiment, the high dose of HYSA/scaffolds has a significantly better capacity to promote new bone formation than the undoped scaffolds at 8 weeks post-surgery.
32562783	9	42	theme	effective	1759:1767	arg1	biomaterials					1802:1813	effective and safe bone tissue engineering biomaterials	1759:1813	effective and safe bone tissue engineering biomaterials for bone regeneration	1759:1835	Thus, our results claimed that the novel HYSA/scaffolds hold the substantial potential to be further developed as effective and safe bone tissue engineering biomaterials for bone regeneration by combining enhanced osteogenesis and angiogenesis.
32562783	1	43	theme	yellow	223:228	arg1	A					230:230	hydroxy-safflower yellow A	205:230	hydroxy-safflower yellow A (HYSA)	205:237	Some traditional Chinese medicine (TCM) has been applied in bone repair, however, hydroxy-safflower yellow A (HYSA), one composition of safflower of the typical invigorating the circulation of TCM, has little been studied in orthopedics field for osteogenesis and angiogenesis clinically.
32562783	1	43	theme	yellow	223:228	arg1	composition					244:254	one composition	240:254	one composition of safflower of the typical invigorating the circulation of TCM	240:318	Some traditional Chinese medicine (TCM) has been applied in bone repair, however, hydroxy-safflower yellow A (HYSA), one composition of safflower of the typical invigorating the circulation of TCM, has little been studied in orthopedics field for osteogenesis and angiogenesis clinically.
32562783	1	43	theme	yellow	223:228	arg1	HYSA					233:236	HYSA	233:236	HYSA	233:236	Some traditional Chinese medicine (TCM) has been applied in bone repair, however, hydroxy-safflower yellow A (HYSA), one composition of safflower of the typical invigorating the circulation of TCM, has little been studied in orthopedics field for osteogenesis and angiogenesis clinically.
32562783	5	44	theme	cells	1074:1078	arg1	migration					1093:1101	migration	1093:1101	migration	1093:1101	In vitro the cell culture results exhibited that the high dose of HYSA (0.5 mg/ml) loaded scaffolds can promote the proliferation of bone marrow stromal cells (rBMSCs) and migration, tubule formation of human umbilical vein endothelial cells (HUVECs).
32562783	5	44	theme	cells	1074:1078	arg1	formation					1111:1119	tubule formation	1104:1119	tubule formation of human umbilical vein endothelial cells (HUVECs)	1104:1170	In vitro the cell culture results exhibited that the high dose of HYSA (0.5 mg/ml) loaded scaffolds can promote the proliferation of bone marrow stromal cells (rBMSCs) and migration, tubule formation of human umbilical vein endothelial cells (HUVECs).
32562783	5	44	theme	cells	1074:1078	arg1	proliferation					1037:1049	the proliferation	1033:1049	the proliferation of bone marrow stromal cells (rBMSCs)	1033:1087	In vitro the cell culture results exhibited that the high dose of HYSA (0.5 mg/ml) loaded scaffolds can promote the proliferation of bone marrow stromal cells (rBMSCs) and migration, tubule formation of human umbilical vein endothelial cells (HUVECs).
32562783	9	45	theme	safe	1773:1776	arg1	biomaterials					1802:1813	effective and safe bone tissue engineering biomaterials	1759:1813	effective and safe bone tissue engineering biomaterials for bone regeneration	1759:1835	Thus, our results claimed that the novel HYSA/scaffolds hold the substantial potential to be further developed as effective and safe bone tissue engineering biomaterials for bone regeneration by combining enhanced osteogenesis and angiogenesis.
32562783	3	46	theme	excellent	653:661	arg1	control					668:674	excellent drug control	653:674	excellent drug control	653:674	Notably, scaffolds coating chitosan/sodium alginate endowed with excellent drug control release ability, and significantly improved the BG mechanical strength.
32562783	8	47	theme	new	1574:1576	arg1	formation					1583:1591	new bone formation	1574:1591	new bone formation	1574:1591	In the animal experiment, the high dose of HYSA/scaffolds has a significantly better capacity to promote new bone formation than the undoped scaffolds at 8 weeks post-surgery.
32562783	5	48	theme	endothelial	1145:1155	arg1	HUVECs					1164:1169	HUVECs	1164:1169	HUVECs	1164:1169	In vitro the cell culture results exhibited that the high dose of HYSA (0.5 mg/ml) loaded scaffolds can promote the proliferation of bone marrow stromal cells (rBMSCs) and migration, tubule formation of human umbilical vein endothelial cells (HUVECs).
32562783	5	48	theme	endothelial	1145:1155	arg1	cells					1157:1161	human umbilical vein endothelial cells	1124:1161	human umbilical vein endothelial cells (HUVECs)	1124:1170	In vitro the cell culture results exhibited that the high dose of HYSA (0.5 mg/ml) loaded scaffolds can promote the proliferation of bone marrow stromal cells (rBMSCs) and migration, tubule formation of human umbilical vein endothelial cells (HUVECs).
32562783	2	49	theme	3D	525:526	arg1	technique					534:542	a 3D print technique	523:542	a 3D print technique	523:542	Herein, we hypothetically speculated that the synthetic bioactive glasses (BG, 1393) scaffolds carried HYSA by a 3D print technique could enhance osteogenic repair properties.
32562783	9	50	theme	tissue	1783:1788	arg1	biomaterials					1802:1813	effective and safe bone tissue engineering biomaterials	1759:1813	effective and safe bone tissue engineering biomaterials for bone regeneration	1759:1835	Thus, our results claimed that the novel HYSA/scaffolds hold the substantial potential to be further developed as effective and safe bone tissue engineering biomaterials for bone regeneration by combining enhanced osteogenesis and angiogenesis.
32562783	8	51	theme	HYSA/scaffolds	1512:1525	arg1	dose					1504:1507	the high dose	1495:1507	the high dose of HYSA/scaffolds	1495:1525	In the animal experiment, the high dose of HYSA/scaffolds has a significantly better capacity to promote new bone formation than the undoped scaffolds at 8 weeks post-surgery.
32562783	7	52	theme	HYSA/scaffolds	1346:1359	arg1	dose					1338:1341	the high dose	1329:1341	the high dose of HYSA/scaffolds	1329:1359	Results of qRT-PCR and Western blot indicated that the high dose of HYSA/scaffolds can up-regulate ALP, OCN, OPN and RUNX-2 expression and relative protein secretion of the HIF-1α and BMP-2.
32562783	2	53	theme	repair	569:574	arg1	properties					576:585	osteogenic repair properties	558:585	osteogenic repair properties	558:585	Herein, we hypothetically speculated that the synthetic bioactive glasses (BG, 1393) scaffolds carried HYSA by a 3D print technique could enhance osteogenic repair properties.
32562783	7	54	theme	high	1333:1336	arg1	dose					1338:1341	the high dose	1329:1341	the high dose of HYSA/scaffolds	1329:1359	Results of qRT-PCR and Western blot indicated that the high dose of HYSA/scaffolds can up-regulate ALP, OCN, OPN and RUNX-2 expression and relative protein secretion of the HIF-1α and BMP-2.
32562783	6	55	theme	active	1177:1182	arg1	ALP					1206:1208	ALP	1206:1208	ALP	1206:1208	The active alkaline phosphatase (ALP) of rBMSCs can also be improved by the high dose of HYSA/scaffolds.
32562783	6	55	theme	active	1177:1182	arg1	phosphatase					1193:1203	The active alkaline phosphatase	1173:1203	The active alkaline phosphatase (ALP) of rBMSCs	1173:1219	The active alkaline phosphatase (ALP) of rBMSCs can also be improved by the high dose of HYSA/scaffolds.
32562783	3	56	with	endowed	640:646	arg1	control					668:674	excellent drug control	653:674	excellent drug control	653:674	Notably, scaffolds coating chitosan/sodium alginate endowed with excellent drug control release ability, and significantly improved the BG mechanical strength.
32562783	5	57	theme	umbilical	1130:1138	arg1	HUVECs					1164:1169	HUVECs	1164:1169	HUVECs	1164:1169	In vitro the cell culture results exhibited that the high dose of HYSA (0.5 mg/ml) loaded scaffolds can promote the proliferation of bone marrow stromal cells (rBMSCs) and migration, tubule formation of human umbilical vein endothelial cells (HUVECs).
32562783	5	57	theme	umbilical	1130:1138	arg1	cells					1157:1161	human umbilical vein endothelial cells	1124:1161	human umbilical vein endothelial cells (HUVECs)	1124:1170	In vitro the cell culture results exhibited that the high dose of HYSA (0.5 mg/ml) loaded scaffolds can promote the proliferation of bone marrow stromal cells (rBMSCs) and migration, tubule formation of human umbilical vein endothelial cells (HUVECs).
32562783	9	58	theme	bone	1819:1822	arg1	regeneration					1824:1835	bone regeneration	1819:1835	bone regeneration	1819:1835	Thus, our results claimed that the novel HYSA/scaffolds hold the substantial potential to be further developed as effective and safe bone tissue engineering biomaterials for bone regeneration by combining enhanced osteogenesis and angiogenesis.
32562783	3	59	theme	BG	724:725	arg1	strength					738:745	the BG mechanical strength	720:745	the BG mechanical strength	720:745	Notably, scaffolds coating chitosan/sodium alginate endowed with excellent drug control release ability, and significantly improved the BG mechanical strength.
32562783	6	60	theme	high	1249:1252	arg1	dose					1254:1257	the high dose	1245:1257	the high dose of HYSA/scaffolds	1245:1275	The active alkaline phosphatase (ALP) of rBMSCs can also be improved by the high dose of HYSA/scaffolds.
32562783	7	61	theme	blot	1309:1312	arg1	Results					1278:1284	Results	1278:1284	Results of qRT-PCR and Western blot	1278:1312	Results of qRT-PCR and Western blot indicated that the high dose of HYSA/scaffolds can up-regulate ALP, OCN, OPN and RUNX-2 expression and relative protein secretion of the HIF-1α and BMP-2.
32562783	1	62	theme	bone	183:186	arg1	repair					188:193	bone repair	183:193	bone repair	183:193	Some traditional Chinese medicine (TCM) has been applied in bone repair, however, hydroxy-safflower yellow A (HYSA), one composition of safflower of the typical invigorating the circulation of TCM, has little been studied in orthopedics field for osteogenesis and angiogenesis clinically.
32562783	9	63	theme	enhanced	1850:1857	arg1	osteogenesis					1859:1870	osteogenesis	1859:1870	osteogenesis	1859:1870	Thus, our results claimed that the novel HYSA/scaffolds hold the substantial potential to be further developed as effective and safe bone tissue engineering biomaterials for bone regeneration by combining enhanced osteogenesis and angiogenesis.
32562783	8	64	theme	post-surgery	1631:1642	arg1	weeks					1625:1629	8 weeks post-surgery	1623:1642	8 weeks post-surgery	1623:1642	In the animal experiment, the high dose of HYSA/scaffolds has a significantly better capacity to promote new bone formation than the undoped scaffolds at 8 weeks post-surgery.
32562783	7	65	dep	HIF-1α	1451:1456	arg1	the					1447:1449	the	1447:1449	the	1447:1449	Results of qRT-PCR and Western blot indicated that the high dose of HYSA/scaffolds can up-regulate ALP, OCN, OPN and RUNX-2 expression and relative protein secretion of the HIF-1α and BMP-2.
32562783	4	66	theme	HYSA/scaffolds	869:882	arg1	osteogenesis					832:843	osteogenesis	832:843	osteogenesis	832:843	HYSA was loaded into BG scaffolds by coating chitosan/sodium alginate film, and the osteogenesis and angiogenesis of the HYSA/scaffolds were evaluated in vitro and in vivo.
32562783	4	66	theme	HYSA/scaffolds	869:882	arg1	angiogenesis					849:860	angiogenesis	849:860	angiogenesis	849:860	HYSA was loaded into BG scaffolds by coating chitosan/sodium alginate film, and the osteogenesis and angiogenesis of the HYSA/scaffolds were evaluated in vitro and in vivo.
32562783	5	67	theme	loaded	1004:1009	arg1	scaffolds					1011:1019	HYSA (0.5 mg/ml) loaded scaffolds	987:1019	HYSA (0.5 mg/ml) loaded scaffolds	987:1019	In vitro the cell culture results exhibited that the high dose of HYSA (0.5 mg/ml) loaded scaffolds can promote the proliferation of bone marrow stromal cells (rBMSCs) and migration, tubule formation of human umbilical vein endothelial cells (HUVECs).
32562783	1	68	theme	traditional	128:138	arg1	TCM					158:160	TCM	158:160	TCM	158:160	Some traditional Chinese medicine (TCM) has been applied in bone repair, however, hydroxy-safflower yellow A (HYSA), one composition of safflower of the typical invigorating the circulation of TCM, has little been studied in orthopedics field for osteogenesis and angiogenesis clinically.
32562783	1	68	theme	traditional	128:138	arg1	medicine					148:155	Some traditional Chinese medicine	123:155	Some traditional Chinese medicine (TCM)	123:161	Some traditional Chinese medicine (TCM) has been applied in bone repair, however, hydroxy-safflower yellow A (HYSA), one composition of safflower of the typical invigorating the circulation of TCM, has little been studied in orthopedics field for osteogenesis and angiogenesis clinically.
32562783	6	69	theme	HYSA/scaffolds	1262:1275	arg1	dose					1254:1257	the high dose	1245:1257	the high dose of HYSA/scaffolds	1245:1275	The active alkaline phosphatase (ALP) of rBMSCs can also be improved by the high dose of HYSA/scaffolds.
32562783	0	70	theme	ancient	76:82	arg1	medicine					92:99	ancient Chinese medicine	76:99	ancient Chinese medicine HYSA for bone repair	76:120	A novel 3D printed bioactive scaffolds with enhanced osteogenic inspired by ancient Chinese medicine HYSA for bone repair.
32562783	1	71	theme	invigorating	284:295	arg1	circulation					301:311	the typical invigorating the circulation	272:311	the typical invigorating the circulation of TCM	272:318	Some traditional Chinese medicine (TCM) has been applied in bone repair, however, hydroxy-safflower yellow A (HYSA), one composition of safflower of the typical invigorating the circulation of TCM, has little been studied in orthopedics field for osteogenesis and angiogenesis clinically.
32562783	0	72	theme	medicine	92:99	arg1	HYSA					101:104	ancient Chinese medicine HYSA	76:104	ancient Chinese medicine HYSA for bone repair	76:120	A novel 3D printed bioactive scaffolds with enhanced osteogenic inspired by ancient Chinese medicine HYSA for bone repair.
32562783	1	73	theme	circulation	301:311	arg1	safflower					259:267	safflower	259:267	safflower of the typical invigorating the circulation of TCM	259:318	Some traditional Chinese medicine (TCM) has been applied in bone repair, however, hydroxy-safflower yellow A (HYSA), one composition of safflower of the typical invigorating the circulation of TCM, has little been studied in orthopedics field for osteogenesis and angiogenesis clinically.
32562783	1	74	theme	TCM	316:318	arg1	circulation					301:311	the typical invigorating the circulation	272:311	the typical invigorating the circulation of TCM	272:318	Some traditional Chinese medicine (TCM) has been applied in bone repair, however, hydroxy-safflower yellow A (HYSA), one composition of safflower of the typical invigorating the circulation of TCM, has little been studied in orthopedics field for osteogenesis and angiogenesis clinically.
32562783	5	75	theme	cell	934:937	arg1	culture					939:945	the cell culture	930:945	the cell culture	930:945	In vitro the cell culture results exhibited that the high dose of HYSA (0.5 mg/ml) loaded scaffolds can promote the proliferation of bone marrow stromal cells (rBMSCs) and migration, tubule formation of human umbilical vein endothelial cells (HUVECs).
32562783	2	76	theme	bioactive	468:476	arg1	glasses					478:484	synthetic bioactive glasses	458:484	the synthetic bioactive glasses (BG, 1393) scaffolds carried HYSA by a 3D print technique	454:542	Herein, we hypothetically speculated that the synthetic bioactive glasses (BG, 1393) scaffolds carried HYSA by a 3D print technique could enhance osteogenic repair properties.
32562783	0	77	theme	novel	2:6	arg1	scaffolds					29:37	A novel 3D printed bioactive scaffolds	0:37	A novel 3D printed bioactive scaffolds with enhanced osteogenic	0:62	A novel 3D printed bioactive scaffolds with enhanced osteogenic inspired by ancient Chinese medicine HYSA for bone repair.
32562783	5	78	theme	marrow	1059:1064	arg1	cells					1074:1078	bone marrow stromal cells	1054:1078	bone marrow stromal cells (rBMSCs)	1054:1087	In vitro the cell culture results exhibited that the high dose of HYSA (0.5 mg/ml) loaded scaffolds can promote the proliferation of bone marrow stromal cells (rBMSCs) and migration, tubule formation of human umbilical vein endothelial cells (HUVECs).
32562783	5	78	theme	marrow	1059:1064	arg1	rBMSCs					1081:1086	rBMSCs	1081:1086	rBMSCs	1081:1086	In vitro the cell culture results exhibited that the high dose of HYSA (0.5 mg/ml) loaded scaffolds can promote the proliferation of bone marrow stromal cells (rBMSCs) and migration, tubule formation of human umbilical vein endothelial cells (HUVECs).
32562783	9	79	theme	novel	1680:1684	arg1	HYSA/scaffolds					1686:1699	the novel HYSA/scaffolds	1676:1699	the novel HYSA/scaffolds	1676:1699	Thus, our results claimed that the novel HYSA/scaffolds hold the substantial potential to be further developed as effective and safe bone tissue engineering biomaterials for bone regeneration by combining enhanced osteogenesis and angiogenesis.
32562783	0	80	theme	printed	11:17	arg1	scaffolds					29:37	A novel 3D printed bioactive scaffolds	0:37	A novel 3D printed bioactive scaffolds with enhanced osteogenic	0:62	A novel 3D printed bioactive scaffolds with enhanced osteogenic inspired by ancient Chinese medicine HYSA for bone repair.
32562783	2	81	dep	glasses	478:484	arg1	1393					491:494	1393	491:494	1393	491:494	Herein, we hypothetically speculated that the synthetic bioactive glasses (BG, 1393) scaffolds carried HYSA by a 3D print technique could enhance osteogenic repair properties.
32562783	2	81	dep	glasses	478:484	arg1	BG					487:488	BG	487:488	BG	487:488	Herein, we hypothetically speculated that the synthetic bioactive glasses (BG, 1393) scaffolds carried HYSA by a 3D print technique could enhance osteogenic repair properties.
32562783	5	82	theme	human	1124:1128	arg1	HUVECs					1164:1169	HUVECs	1164:1169	HUVECs	1164:1169	In vitro the cell culture results exhibited that the high dose of HYSA (0.5 mg/ml) loaded scaffolds can promote the proliferation of bone marrow stromal cells (rBMSCs) and migration, tubule formation of human umbilical vein endothelial cells (HUVECs).
32562783	5	82	theme	human	1124:1128	arg1	cells					1157:1161	human umbilical vein endothelial cells	1124:1161	human umbilical vein endothelial cells (HUVECs)	1124:1170	In vitro the cell culture results exhibited that the high dose of HYSA (0.5 mg/ml) loaded scaffolds can promote the proliferation of bone marrow stromal cells (rBMSCs) and migration, tubule formation of human umbilical vein endothelial cells (HUVECs).
32562783	5	83	theme	high	974:977	arg1	dose					979:982	the high dose	970:982	the high dose of HYSA (0.5 mg/ml) loaded scaffolds	970:1019	In vitro the cell culture results exhibited that the high dose of HYSA (0.5 mg/ml) loaded scaffolds can promote the proliferation of bone marrow stromal cells (rBMSCs) and migration, tubule formation of human umbilical vein endothelial cells (HUVECs).
32562783	9	84	theme	substantial	1710:1720	arg1	potential					1722:1730	the substantial potential	1706:1730	the substantial potential to be further developed as effective and safe bone tissue engineering biomaterials for bone regeneration by combining enhanced osteogenesis and angiogenesis	1706:1887	Thus, our results claimed that the novel HYSA/scaffolds hold the substantial potential to be further developed as effective and safe bone tissue engineering biomaterials for bone regeneration by combining enhanced osteogenesis and angiogenesis.
32562783	4	85	dep	osteogenesis	832:843	arg1	the					828:830	the	828:830	the	828:830	HYSA was loaded into BG scaffolds by coating chitosan/sodium alginate film, and the osteogenesis and angiogenesis of the HYSA/scaffolds were evaluated in vitro and in vivo.
32562783	1	86	theme	safflower	259:267	arg1	A					230:230	hydroxy-safflower yellow A	205:230	hydroxy-safflower yellow A (HYSA)	205:237	Some traditional Chinese medicine (TCM) has been applied in bone repair, however, hydroxy-safflower yellow A (HYSA), one composition of safflower of the typical invigorating the circulation of TCM, has little been studied in orthopedics field for osteogenesis and angiogenesis clinically.
32562783	1	86	theme	safflower	259:267	arg1	composition					244:254	one composition	240:254	one composition of safflower of the typical invigorating the circulation of TCM	240:318	Some traditional Chinese medicine (TCM) has been applied in bone repair, however, hydroxy-safflower yellow A (HYSA), one composition of safflower of the typical invigorating the circulation of TCM, has little been studied in orthopedics field for osteogenesis and angiogenesis clinically.
32562783	7	87	theme	protein	1426:1432	arg1	secretion					1434:1442	relative protein secretion	1417:1442	relative protein secretion of the HIF-1α and BMP-2	1417:1466	Results of qRT-PCR and Western blot indicated that the high dose of HYSA/scaffolds can up-regulate ALP, OCN, OPN and RUNX-2 expression and relative protein secretion of the HIF-1α and BMP-2.
32562783	8	88	theme	animal	1476:1481	arg1	experiment					1483:1492	the animal experiment	1472:1492	the animal experiment	1472:1492	In the animal experiment, the high dose of HYSA/scaffolds has a significantly better capacity to promote new bone formation than the undoped scaffolds at 8 weeks post-surgery.
32562783	7	89	theme	RUNX-2	1395:1400	arg1	expression					1402:1411	ALP, OCN, OPN and RUNX-2 expression	1377:1411	expression	1402:1411	Results of qRT-PCR and Western blot indicated that the high dose of HYSA/scaffolds can up-regulate ALP, OCN, OPN and RUNX-2 expression and relative protein secretion of the HIF-1α and BMP-2.
32562783	8	90	theme	high	1499:1502	arg1	dose					1504:1507	the high dose	1495:1507	the high dose of HYSA/scaffolds	1495:1525	In the animal experiment, the high dose of HYSA/scaffolds has a significantly better capacity to promote new bone formation than the undoped scaffolds at 8 weeks post-surgery.
32562783	3	91	theme	drug	663:666	arg1	control					668:674	excellent drug control	653:674	excellent drug control	653:674	Notably, scaffolds coating chitosan/sodium alginate endowed with excellent drug control release ability, and significantly improved the BG mechanical strength.
32562783	0	92	with	scaffolds	29:37	arg1	osteogenic					53:62	osteogenic	53:62	osteogenic	53:62	A novel 3D printed bioactive scaffolds with enhanced osteogenic inspired by ancient Chinese medicine HYSA for bone repair.
32562783	1	93	theme	hydroxy-safflower	205:221	arg1	A					230:230	hydroxy-safflower yellow A	205:230	hydroxy-safflower yellow A (HYSA)	205:237	Some traditional Chinese medicine (TCM) has been applied in bone repair, however, hydroxy-safflower yellow A (HYSA), one composition of safflower of the typical invigorating the circulation of TCM, has little been studied in orthopedics field for osteogenesis and angiogenesis clinically.
32562783	1	93	theme	hydroxy-safflower	205:221	arg1	composition					244:254	one composition	240:254	one composition of safflower of the typical invigorating the circulation of TCM	240:318	Some traditional Chinese medicine (TCM) has been applied in bone repair, however, hydroxy-safflower yellow A (HYSA), one composition of safflower of the typical invigorating the circulation of TCM, has little been studied in orthopedics field for osteogenesis and angiogenesis clinically.
32562783	1	93	theme	hydroxy-safflower	205:221	arg1	HYSA					233:236	HYSA	233:236	HYSA	233:236	Some traditional Chinese medicine (TCM) has been applied in bone repair, however, hydroxy-safflower yellow A (HYSA), one composition of safflower of the typical invigorating the circulation of TCM, has little been studied in orthopedics field for osteogenesis and angiogenesis clinically.
33214583	6	0	dep	HLF-laden	1222:1230	arg1	fibrin-based					1233:1244	fibrin-based	1233:1244	fibrin-based	1233:1244	Our results indicate that culturing tumor spheroids containing MDA-MB-231 cells + HUVECs in an HLF-laden, fibrin-based ECM within our microfluidic device optimally (1) enhances the sprouting and migration of tumor spheroids, (2) promotes angiogenesis, (3) facilitates vascular invasion, and (4) preserves the structural integrity and functionality of HUVEC-lined microfluidic channels.
33214583	1	1	theme	Molecular	75:83	arg1	crosstalk					85:93	Molecular crosstalk	75:93	Molecular crosstalk between intra-tumor blood vessels and tumor cells	75:143	Molecular crosstalk between intra-tumor blood vessels and tumor cells plays many critical roles in tumorigenesis and cancer metastasis.
33214583	2	2	theme	murine	404:409	arg1	models					411:416	conventional murine models	391:416	conventional murine models	391:416	However, it has been very difficult to investigate the biochemical mechanisms underlying the overlapping, multifactorial processes that occur at the tumor-vascular interface using conventional murine models alone.
33214583	3	3	theme	tumor	545:549	arg1	microenvironment					551:566	the 3D tumor microenvironment	538:566	the 3D tumor microenvironment	538:566	Moreover, traditional two-dimensional (2D) culture models used in cancer research do not recapitulate aspects of the 3D tumor microenvironment.
33214583	6	4	theme	structural	1436:1445	arg1	integrity					1447:1455	the structural integrity	1432:1455	the structural integrity	1432:1455	Our results indicate that culturing tumor spheroids containing MDA-MB-231 cells + HUVECs in an HLF-laden, fibrin-based ECM within our microfluidic device optimally (1) enhances the sprouting and migration of tumor spheroids, (2) promotes angiogenesis, (3) facilitates vascular invasion, and (4) preserves the structural integrity and functionality of HUVEC-lined microfluidic channels.
33214583	4	5	theme	umbilical	683:691	arg1	cell					710:713	a human umbilical vein endothelial cell	675:713	a human umbilical vein endothelial cell (HUVEC)	675:721	In the present study, we introduce a microfluidic model of the solid tumor-vascular interface composed of a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel and tumor spheroids embedded in an extracellular matrix (ECM).
33214583	4	5	theme	umbilical	683:691	arg1	HUVEC					716:720	HUVEC	716:720	HUVEC	716:720	In the present study, we introduce a microfluidic model of the solid tumor-vascular interface composed of a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel and tumor spheroids embedded in an extracellular matrix (ECM).
33214583	4	6	theme	tumor-vascular	638:651	arg1	interface					653:661	the solid tumor-vascular interface	628:661	the solid tumor-vascular interface composed of a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel and tumor spheroids embedded in an extracellular matrix (ECM)	628:829	In the present study, we introduce a microfluidic model of the solid tumor-vascular interface composed of a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel and tumor spheroids embedded in an extracellular matrix (ECM).
33214583	5	7	theme	breast	926:931	arg1	cells					964:968	MDA-MB-231 breast tumor cells + mesenchymal stem cells	915:968	MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs	915:1012	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33214583	5	7	theme	breast	926:931	arg1	MSCs					971:974	MSCs	971:974	MSCs	971:974	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33214583	5	8	theme	matrix	1037:1042	arg1	composition					879:889	the composition	875:889	the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used	875:1124	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33214583	7	9	theme	drug	1551:1554	arg1	screening					1556:1564	drug screening	1551:1564	drug screening	1551:1564	This model may provide a platform for drug screening and mechanism studies on solid tumor interactions with functional blood vessels.
33214583	2	10	dep	overlapping	304:314	arg1	multifactorial					317:330	multifactorial	317:330	multifactorial	317:330	However, it has been very difficult to investigate the biochemical mechanisms underlying the overlapping, multifactorial processes that occur at the tumor-vascular interface using conventional murine models alone.
33214583	5	11	theme	tumor	898:902	arg1	spheroids					904:912	the tumor spheroids	894:912	the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs)	894:1013	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33214583	5	11	theme	tumor	898:902	arg1	/HUVECs					1006:1012	MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs	915:1012	MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs	915:1012	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33214583	7	12	with	interactions	1603:1614	arg1	vessels					1638:1644	functional blood vessels	1621:1644	functional blood vessels	1621:1644	This model may provide a platform for drug screening and mechanism studies on solid tumor interactions with functional blood vessels.
33214583	4	13	from	matrix	818:823	arg1	embedded					789:796	embedded	789:796	embedded	789:796	In the present study, we introduce a microfluidic model of the solid tumor-vascular interface composed of a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel and tumor spheroids embedded in an extracellular matrix (ECM).
33214583	6	14	theme	microfluidic	1490:1501	arg1	channels					1503:1510	HUVEC-lined microfluidic channels	1478:1510	HUVEC-lined microfluidic channels	1478:1510	Our results indicate that culturing tumor spheroids containing MDA-MB-231 cells + HUVECs in an HLF-laden, fibrin-based ECM within our microfluidic device optimally (1) enhances the sprouting and migration of tumor spheroids, (2) promotes angiogenesis, (3) facilitates vascular invasion, and (4) preserves the structural integrity and functionality of HUVEC-lined microfluidic channels.
33214583	1	15	theme	tumor	133:137	arg1	cells					139:143	tumor cells	133:143	tumor cells	133:143	Molecular crosstalk between intra-tumor blood vessels and tumor cells plays many critical roles in tumorigenesis and cancer metastasis.
33214583	4	16	theme	endothelial	698:708	arg1	cell					710:713	a human umbilical vein endothelial cell	675:713	a human umbilical vein endothelial cell (HUVEC)	675:721	In the present study, we introduce a microfluidic model of the solid tumor-vascular interface composed of a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel and tumor spheroids embedded in an extracellular matrix (ECM).
33214583	4	16	theme	endothelial	698:708	arg1	HUVEC					716:720	HUVEC	716:720	HUVEC	716:720	In the present study, we introduce a microfluidic model of the solid tumor-vascular interface composed of a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel and tumor spheroids embedded in an extracellular matrix (ECM).
33214583	0	17	theme	perfused	51:58	arg1	vessels					66:72	perfused blood vessels	51:72	perfused blood vessels	51:72	In vitro modeling of solid tumor interactions with perfused blood vessels.
33214583	1	18	theme	critical	156:163	arg1	roles					165:169	many critical roles	151:169	many critical roles	151:169	Molecular crosstalk between intra-tumor blood vessels and tumor cells plays many critical roles in tumorigenesis and cancer metastasis.
33214583	7	19	theme	blood	1632:1636	arg1	vessels					1638:1644	functional blood vessels	1621:1644	functional blood vessels	1621:1644	This model may provide a platform for drug screening and mechanism studies on solid tumor interactions with functional blood vessels.
33214583	1	20	theme	intra-tumor	103:113	arg1	vessels					121:127	intra-tumor blood vessels	103:127	intra-tumor blood vessels	103:127	Molecular crosstalk between intra-tumor blood vessels and tumor cells plays many critical roles in tumorigenesis and cancer metastasis.
33214583	5	21	theme	collagen	1050:1057	arg1	Matrigel					1060:1067	collagen, Matrigel, and fibrin gels	1050:1084	Matrigel	1060:1067	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33214583	7	22	from	platform	1538:1545	arg1	interactions					1603:1614	solid tumor interactions	1591:1614	solid tumor interactions with functional blood vessels	1591:1644	This model may provide a platform for drug screening and mechanism studies on solid tumor interactions with functional blood vessels.
33214583	5	23	theme	cells	964:968	arg1	spheroids					904:912	the tumor spheroids	894:912	the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs)	894:1013	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33214583	5	23	theme	cells	964:968	arg1	/HUVECs					1006:1012	MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs	915:1012	MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs	915:1012	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33214583	7	24	theme	tumor	1597:1601	arg1	interactions					1603:1614	solid tumor interactions	1591:1614	solid tumor interactions with functional blood vessels	1591:1644	This model may provide a platform for drug screening and mechanism studies on solid tumor interactions with functional blood vessels.
33214583	0	25	theme	blood	60:64	arg1	vessels					66:72	perfused blood vessels	51:72	perfused blood vessels	51:72	In vitro modeling of solid tumor interactions with perfused blood vessels.
33214583	3	26	theme	two-dimensional	447:461	arg1	models					476:481	traditional two-dimensional (2D) culture models	435:481	traditional two-dimensional (2D) culture models used in cancer research	435:505	Moreover, traditional two-dimensional (2D) culture models used in cancer research do not recapitulate aspects of the 3D tumor microenvironment.
33214583	0	27	theme	In	0:1	arg1	modeling					9:16	In vitro modeling	0:16	In vitro modeling of solid tumor interactions with perfused blood vessels	0:72	In vitro modeling of solid tumor interactions with perfused blood vessels.
33214583	4	28	dep	-lined	722:727	arg1	bioengineered					742:754	bioengineered	742:754	bioengineered	742:754	In the present study, we introduce a microfluidic model of the solid tumor-vascular interface composed of a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel and tumor spheroids embedded in an extracellular matrix (ECM).
33214583	4	28	dep	-lined	722:727	arg1	perfusable					730:739	perfusable	730:739	perfusable	730:739	In the present study, we introduce a microfluidic model of the solid tumor-vascular interface composed of a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel and tumor spheroids embedded in an extracellular matrix (ECM).
33214583	6	29	contain	containing	1179:1188	arg2	cells + HUVECs					1201:1214	MDA-MB-231 cells + HUVECs	1190:1214	MDA-MB-231 cells + HUVECs	1190:1214	Our results indicate that culturing tumor spheroids containing MDA-MB-231 cells + HUVECs in an HLF-laden, fibrin-based ECM within our microfluidic device optimally (1) enhances the sprouting and migration of tumor spheroids, (2) promotes angiogenesis, (3) facilitates vascular invasion, and (4) preserves the structural integrity and functionality of HUVEC-lined microfluidic channels.
33214583	6	29	contain	containing	1179:1188	arg1	spheroids					1169:1177	tumor spheroids	1163:1177	tumor spheroids containing MDA-MB-231 cells + HUVECs in an HLF-laden, fibrin-based ECM within our microfluidic device	1163:1279	Our results indicate that culturing tumor spheroids containing MDA-MB-231 cells + HUVECs in an HLF-laden, fibrin-based ECM within our microfluidic device optimally (1) enhances the sprouting and migration of tumor spheroids, (2) promotes angiogenesis, (3) facilitates vascular invasion, and (4) preserves the structural integrity and functionality of HUVEC-lined microfluidic channels.
33214583	4	30	theme	blood	756:760	arg1	vessel					762:767	a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel	675:767	a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel	675:767	In the present study, we introduce a microfluidic model of the solid tumor-vascular interface composed of a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel and tumor spheroids embedded in an extracellular matrix (ECM).
33214583	3	31	theme	3D	542:543	arg1	microenvironment					551:566	the 3D tumor microenvironment	538:566	the 3D tumor microenvironment	538:566	Moreover, traditional two-dimensional (2D) culture models used in cancer research do not recapitulate aspects of the 3D tumor microenvironment.
33214583	5	32	theme	cells + mesenchymal	939:957	arg1	cells					964:968	MDA-MB-231 breast tumor cells + mesenchymal stem cells	915:968	MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs	915:1012	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33214583	5	32	theme	cells + mesenchymal	939:957	arg1	MSCs					971:974	MSCs	971:974	MSCs	971:974	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33214583	7	33	theme	mechanism	1570:1578	arg1	studies					1580:1586	mechanism studies	1570:1586	mechanism studies	1570:1586	This model may provide a platform for drug screening and mechanism studies on solid tumor interactions with functional blood vessels.
33214583	4	34	theme	present	576:582	arg1	study					584:588	the present study	572:588	the present study	572:588	In the present study, we introduce a microfluidic model of the solid tumor-vascular interface composed of a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel and tumor spheroids embedded in an extracellular matrix (ECM).
33214583	3	35	theme	culture	468:474	arg1	models					476:481	traditional two-dimensional (2D) culture models	435:481	traditional two-dimensional (2D) culture models used in cancer research	435:505	Moreover, traditional two-dimensional (2D) culture models used in cancer research do not recapitulate aspects of the 3D tumor microenvironment.
33214583	0	36	theme	solid	21:25	arg1	interactions					33:44	solid tumor interactions	21:44	solid tumor interactions	21:44	In vitro modeling of solid tumor interactions with perfused blood vessels.
33214583	0	37	with	modeling	9:16	arg1	vessels					66:72	perfused blood vessels	51:72	perfused blood vessels	51:72	In vitro modeling of solid tumor interactions with perfused blood vessels.
33214583	6	38	theme	spheroids	1341:1349	arg1	migration					1322:1330	migration	1322:1330	migration	1322:1330	Our results indicate that culturing tumor spheroids containing MDA-MB-231 cells + HUVECs in an HLF-laden, fibrin-based ECM within our microfluidic device optimally (1) enhances the sprouting and migration of tumor spheroids, (2) promotes angiogenesis, (3) facilitates vascular invasion, and (4) preserves the structural integrity and functionality of HUVEC-lined microfluidic channels.
33214583	6	38	theme	spheroids	1341:1349	arg1	sprouting					1308:1316	the sprouting	1304:1316	the sprouting	1304:1316	Our results indicate that culturing tumor spheroids containing MDA-MB-231 cells + HUVECs in an HLF-laden, fibrin-based ECM within our microfluidic device optimally (1) enhances the sprouting and migration of tumor spheroids, (2) promotes angiogenesis, (3) facilitates vascular invasion, and (4) preserves the structural integrity and functionality of HUVEC-lined microfluidic channels.
33214583	5	39	theme	free	1102:1105	arg1	HLFs					1107:1110	free HLFs	1102:1110	free HLFs	1102:1110	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33214583	6	40	dep	facilitates	1383:1393	arg1	3					1380:1380	3	1380:1380	3	1380:1380	Our results indicate that culturing tumor spheroids containing MDA-MB-231 cells + HUVECs in an HLF-laden, fibrin-based ECM within our microfluidic device optimally (1) enhances the sprouting and migration of tumor spheroids, (2) promotes angiogenesis, (3) facilitates vascular invasion, and (4) preserves the structural integrity and functionality of HUVEC-lined microfluidic channels.
33214583	3	41	theme	cancer	491:496	arg1	research					498:505	cancer research	491:505	cancer research	491:505	Moreover, traditional two-dimensional (2D) culture models used in cancer research do not recapitulate aspects of the 3D tumor microenvironment.
33214583	5	42	theme	/human	976:981	arg1	HLFs					1001:1004	HLFs	1001:1004	HLFs	1001:1004	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33214583	5	42	theme	/human	976:981	arg1	fibroblasts					988:998	/human lung fibroblasts	976:998	MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs	915:1012	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33214583	4	43	theme	microfluidic	606:617	arg1	model					619:623	a microfluidic model	604:623	a microfluidic model of the solid tumor-vascular interface composed of a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel and tumor spheroids embedded in an extracellular matrix (ECM)	604:829	In the present study, we introduce a microfluidic model of the solid tumor-vascular interface composed of a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel and tumor spheroids embedded in an extracellular matrix (ECM).
33214583	4	44	theme	extracellular	804:816	arg1	ECM					826:828	ECM	826:828	ECM	826:828	In the present study, we introduce a microfluidic model of the solid tumor-vascular interface composed of a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel and tumor spheroids embedded in an extracellular matrix (ECM).
33214583	4	44	theme	extracellular	804:816	arg1	matrix					818:823	an extracellular matrix	801:823	an extracellular matrix (ECM)	801:829	In the present study, we introduce a microfluidic model of the solid tumor-vascular interface composed of a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel and tumor spheroids embedded in an extracellular matrix (ECM).
33214583	6	45	theme	vascular	1395:1402	arg1	invasion					1404:1411	vascular invasion	1395:1411	vascular invasion	1395:1411	Our results indicate that culturing tumor spheroids containing MDA-MB-231 cells + HUVECs in an HLF-laden, fibrin-based ECM within our microfluidic device optimally (1) enhances the sprouting and migration of tumor spheroids, (2) promotes angiogenesis, (3) facilitates vascular invasion, and (4) preserves the structural integrity and functionality of HUVEC-lined microfluidic channels.
33214583	5	46	theme	fibroblasts	988:998	arg1	spheroids					904:912	the tumor spheroids	894:912	the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs)	894:1013	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33214583	5	46	theme	fibroblasts	988:998	arg1	/HUVECs					1006:1012	MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs	915:1012	MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs	915:1012	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33214583	2	47	theme	tumor-vascular	360:373	arg1	interface					375:383	the tumor-vascular interface	356:383	the tumor-vascular interface	356:383	However, it has been very difficult to investigate the biochemical mechanisms underlying the overlapping, multifactorial processes that occur at the tumor-vascular interface using conventional murine models alone.
33214583	6	48	theme	HLF-laden	1222:1230	arg1	ECM					1246:1248	an HLF-laden, fibrin-based ECM	1219:1248	an HLF-laden, fibrin-based ECM within our microfluidic device	1219:1279	Our results indicate that culturing tumor spheroids containing MDA-MB-231 cells + HUVECs in an HLF-laden, fibrin-based ECM within our microfluidic device optimally (1) enhances the sprouting and migration of tumor spheroids, (2) promotes angiogenesis, (3) facilitates vascular invasion, and (4) preserves the structural integrity and functionality of HUVEC-lined microfluidic channels.
33214583	3	49	theme	microenvironment	551:566	arg1	aspects					527:533	aspects	527:533	aspects of the 3D tumor microenvironment	527:566	Moreover, traditional two-dimensional (2D) culture models used in cancer research do not recapitulate aspects of the 3D tumor microenvironment.
33214583	6	50	dep	preserves	1422:1430	arg1	4					1419:1419	4	1419:1419	4	1419:1419	Our results indicate that culturing tumor spheroids containing MDA-MB-231 cells + HUVECs in an HLF-laden, fibrin-based ECM within our microfluidic device optimally (1) enhances the sprouting and migration of tumor spheroids, (2) promotes angiogenesis, (3) facilitates vascular invasion, and (4) preserves the structural integrity and functionality of HUVEC-lined microfluidic channels.
33214583	2	51	theme	biochemical	266:276	arg1	mechanisms					278:287	the biochemical mechanisms	262:287	the biochemical mechanisms underlying the overlapping, multifactorial processes that occur at the tumor-vascular interface using conventional murine models	262:416	However, it has been very difficult to investigate the biochemical mechanisms underlying the overlapping, multifactorial processes that occur at the tumor-vascular interface using conventional murine models alone.
33214583	0	52	theme	interactions	33:44	arg1	modeling					9:16	In vitro modeling	0:16	In vitro modeling of solid tumor interactions with perfused blood vessels	0:72	In vitro modeling of solid tumor interactions with perfused blood vessels.
33214583	6	53	theme	MDA-MB-231	1190:1199	arg1	cells + HUVECs					1201:1214	MDA-MB-231 cells + HUVECs	1190:1214	MDA-MB-231 cells + HUVECs	1190:1214	Our results indicate that culturing tumor spheroids containing MDA-MB-231 cells + HUVECs in an HLF-laden, fibrin-based ECM within our microfluidic device optimally (1) enhances the sprouting and migration of tumor spheroids, (2) promotes angiogenesis, (3) facilitates vascular invasion, and (4) preserves the structural integrity and functionality of HUVEC-lined microfluidic channels.
33214583	4	54	theme	human	677:681	arg1	cell					710:713	a human umbilical vein endothelial cell	675:713	a human umbilical vein endothelial cell (HUVEC)	675:721	In the present study, we introduce a microfluidic model of the solid tumor-vascular interface composed of a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel and tumor spheroids embedded in an extracellular matrix (ECM).
33214583	4	54	theme	human	677:681	arg1	HUVEC					716:720	HUVEC	716:720	HUVEC	716:720	In the present study, we introduce a microfluidic model of the solid tumor-vascular interface composed of a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel and tumor spheroids embedded in an extracellular matrix (ECM).
33214583	2	55	theme	overlapping	304:314	arg1	processes					332:340	the overlapping, multifactorial processes	300:340	the overlapping, multifactorial processes that occur at the tumor-vascular interface using conventional murine models	300:416	However, it has been very difficult to investigate the biochemical mechanisms underlying the overlapping, multifactorial processes that occur at the tumor-vascular interface using conventional murine models alone.
33214583	1	56	theme	cancer	192:197	arg1	metastasis					199:208	cancer metastasis	192:208	cancer metastasis	192:208	Molecular crosstalk between intra-tumor blood vessels and tumor cells plays many critical roles in tumorigenesis and cancer metastasis.
33214583	4	57	theme	solid	632:636	arg1	interface					653:661	the solid tumor-vascular interface	628:661	the solid tumor-vascular interface composed of a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel and tumor spheroids embedded in an extracellular matrix (ECM)	628:829	In the present study, we introduce a microfluidic model of the solid tumor-vascular interface composed of a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel and tumor spheroids embedded in an extracellular matrix (ECM).
33214583	6	58	theme	tumor	1163:1167	arg1	spheroids					1169:1177	tumor spheroids	1163:1177	tumor spheroids containing MDA-MB-231 cells + HUVECs in an HLF-laden, fibrin-based ECM within our microfluidic device	1163:1279	Our results indicate that culturing tumor spheroids containing MDA-MB-231 cells + HUVECs in an HLF-laden, fibrin-based ECM within our microfluidic device optimally (1) enhances the sprouting and migration of tumor spheroids, (2) promotes angiogenesis, (3) facilitates vascular invasion, and (4) preserves the structural integrity and functionality of HUVEC-lined microfluidic channels.
33214583	4	59	theme	interface	653:661	arg1	model					619:623	a microfluidic model	604:623	a microfluidic model of the solid tumor-vascular interface composed of a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel and tumor spheroids embedded in an extracellular matrix (ECM)	604:829	In the present study, we introduce a microfluidic model of the solid tumor-vascular interface composed of a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel and tumor spheroids embedded in an extracellular matrix (ECM).
33214583	6	60	theme	microfluidic	1261:1272	arg1	device					1274:1279	our microfluidic device	1257:1279	our microfluidic device	1257:1279	Our results indicate that culturing tumor spheroids containing MDA-MB-231 cells + HUVECs in an HLF-laden, fibrin-based ECM within our microfluidic device optimally (1) enhances the sprouting and migration of tumor spheroids, (2) promotes angiogenesis, (3) facilitates vascular invasion, and (4) preserves the structural integrity and functionality of HUVEC-lined microfluidic channels.
33214583	1	61	theme	blood	115:119	arg1	vessels					121:127	intra-tumor blood vessels	103:127	intra-tumor blood vessels	103:127	Molecular crosstalk between intra-tumor blood vessels and tumor cells plays many critical roles in tumorigenesis and cancer metastasis.
33214583	6	62	theme	channels	1503:1510	arg1	functionality					1461:1473	functionality	1461:1473	functionality	1461:1473	Our results indicate that culturing tumor spheroids containing MDA-MB-231 cells + HUVECs in an HLF-laden, fibrin-based ECM within our microfluidic device optimally (1) enhances the sprouting and migration of tumor spheroids, (2) promotes angiogenesis, (3) facilitates vascular invasion, and (4) preserves the structural integrity and functionality of HUVEC-lined microfluidic channels.
33214583	6	62	theme	channels	1503:1510	arg1	integrity					1447:1455	the structural integrity	1432:1455	the structural integrity	1432:1455	Our results indicate that culturing tumor spheroids containing MDA-MB-231 cells + HUVECs in an HLF-laden, fibrin-based ECM within our microfluidic device optimally (1) enhances the sprouting and migration of tumor spheroids, (2) promotes angiogenesis, (3) facilitates vascular invasion, and (4) preserves the structural integrity and functionality of HUVEC-lined microfluidic channels.
33214583	5	63	theme	spheroids	904:912	arg1	composition					879:889	the composition	875:889	the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used	875:1124	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33214583	4	64	theme	-lined	722:727	arg1	vessel					762:767	a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel	675:767	a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel	675:767	In the present study, we introduce a microfluidic model of the solid tumor-vascular interface composed of a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel and tumor spheroids embedded in an extracellular matrix (ECM).
33214583	6	65	theme	tumor	1335:1339	arg1	spheroids					1341:1349	tumor spheroids	1335:1349	tumor spheroids	1335:1349	Our results indicate that culturing tumor spheroids containing MDA-MB-231 cells + HUVECs in an HLF-laden, fibrin-based ECM within our microfluidic device optimally (1) enhances the sprouting and migration of tumor spheroids, (2) promotes angiogenesis, (3) facilitates vascular invasion, and (4) preserves the structural integrity and functionality of HUVEC-lined microfluidic channels.
33214583	5	66	theme	extracellular	1023:1035	arg1	matrix					1037:1042	the extracellular matrix	1019:1042	the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used	1019:1124	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33214583	6	67	theme	HUVEC-lined	1478:1488	arg1	channels					1503:1510	HUVEC-lined microfluidic channels	1478:1510	HUVEC-lined microfluidic channels	1478:1510	Our results indicate that culturing tumor spheroids containing MDA-MB-231 cells + HUVECs in an HLF-laden, fibrin-based ECM within our microfluidic device optimally (1) enhances the sprouting and migration of tumor spheroids, (2) promotes angiogenesis, (3) facilitates vascular invasion, and (4) preserves the structural integrity and functionality of HUVEC-lined microfluidic channels.
33214583	4	68	theme	vein	693:696	arg1	cell					710:713	a human umbilical vein endothelial cell	675:713	a human umbilical vein endothelial cell (HUVEC)	675:721	In the present study, we introduce a microfluidic model of the solid tumor-vascular interface composed of a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel and tumor spheroids embedded in an extracellular matrix (ECM).
33214583	4	68	theme	vein	693:696	arg1	HUVEC					716:720	HUVEC	716:720	HUVEC	716:720	In the present study, we introduce a microfluidic model of the solid tumor-vascular interface composed of a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel and tumor spheroids embedded in an extracellular matrix (ECM).
33214583	5	69	theme	fibrin	1074:1079	arg1	gels					1081:1084	collagen, Matrigel, and fibrin gels	1050:1084	gels	1081:1084	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33214583	6	70	dep	enhances	1295:1302	arg1	promotes					1356:1363	promotes	1356:1363	promotes angiogenesis	1356:1376	Our results indicate that culturing tumor spheroids containing MDA-MB-231 cells + HUVECs in an HLF-laden, fibrin-based ECM within our microfluidic device optimally (1) enhances the sprouting and migration of tumor spheroids, (2) promotes angiogenesis, (3) facilitates vascular invasion, and (4) preserves the structural integrity and functionality of HUVEC-lined microfluidic channels.
33214583	6	70	dep	enhances	1295:1302	arg1	2					1353:1353	2	1353:1353	2	1353:1353	Our results indicate that culturing tumor spheroids containing MDA-MB-231 cells + HUVECs in an HLF-laden, fibrin-based ECM within our microfluidic device optimally (1) enhances the sprouting and migration of tumor spheroids, (2) promotes angiogenesis, (3) facilitates vascular invasion, and (4) preserves the structural integrity and functionality of HUVEC-lined microfluidic channels.
33214583	6	70	dep	enhances	1295:1302	arg1	1					1292:1292	1	1292:1292	1	1292:1292	Our results indicate that culturing tumor spheroids containing MDA-MB-231 cells + HUVECs in an HLF-laden, fibrin-based ECM within our microfluidic device optimally (1) enhances the sprouting and migration of tumor spheroids, (2) promotes angiogenesis, (3) facilitates vascular invasion, and (4) preserves the structural integrity and functionality of HUVEC-lined microfluidic channels.
33214583	4	71	from	embedded	789:796	arg1	ECM					826:828	ECM	826:828	ECM	826:828	In the present study, we introduce a microfluidic model of the solid tumor-vascular interface composed of a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel and tumor spheroids embedded in an extracellular matrix (ECM).
33214583	4	71	from	embedded	789:796	arg1	matrix					818:823	an extracellular matrix	801:823	an extracellular matrix (ECM)	801:829	In the present study, we introduce a microfluidic model of the solid tumor-vascular interface composed of a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel and tumor spheroids embedded in an extracellular matrix (ECM).
33214583	1	72	theme	many	151:154	arg1	roles					165:169	many critical roles	151:169	many critical roles	151:169	Molecular crosstalk between intra-tumor blood vessels and tumor cells plays many critical roles in tumorigenesis and cancer metastasis.
33214583	5	73	theme	stem	959:962	arg1	cells					964:968	MDA-MB-231 breast tumor cells + mesenchymal stem cells	915:968	MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs	915:1012	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33214583	5	73	theme	stem	959:962	arg1	MSCs					971:974	MSCs	971:974	MSCs	971:974	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33214583	7	74	theme	functional	1621:1630	arg1	vessels					1638:1644	functional blood vessels	1621:1644	functional blood vessels	1621:1644	This model may provide a platform for drug screening and mechanism studies on solid tumor interactions with functional blood vessels.
33214583	4	75	theme	tumor	773:777	arg1	spheroids					779:787	tumor spheroids	773:787	tumor spheroids embedded in an extracellular matrix (ECM)	773:829	In the present study, we introduce a microfluidic model of the solid tumor-vascular interface composed of a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel and tumor spheroids embedded in an extracellular matrix (ECM).
33214583	5	76	theme	MDA-MB-231	915:924	arg1	cells					964:968	MDA-MB-231 breast tumor cells + mesenchymal stem cells	915:968	MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs	915:1012	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33214583	5	76	theme	MDA-MB-231	915:924	arg1	MSCs					971:974	MSCs	971:974	MSCs	971:974	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33214583	2	77	theme	conventional	391:402	arg1	models					411:416	conventional murine models	391:416	conventional murine models	391:416	However, it has been very difficult to investigate the biochemical mechanisms underlying the overlapping, multifactorial processes that occur at the tumor-vascular interface using conventional murine models alone.
33214583	5	78	theme	tumor	933:937	arg1	cells					964:968	MDA-MB-231 breast tumor cells + mesenchymal stem cells	915:968	MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs	915:1012	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33214583	5	78	theme	tumor	933:937	arg1	MSCs					971:974	MSCs	971:974	MSCs	971:974	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33214583	7	79	theme	solid	1591:1595	arg1	interactions					1603:1614	solid tumor interactions	1591:1614	solid tumor interactions with functional blood vessels	1591:1644	This model may provide a platform for drug screening and mechanism studies on solid tumor interactions with functional blood vessels.
33214583	0	80	theme	tumor	27:31	arg1	interactions					33:44	solid tumor interactions	21:44	solid tumor interactions	21:44	In vitro modeling of solid tumor interactions with perfused blood vessels.
33214583	3	81	dep	two-dimensional	447:461	arg1	2D					464:465	2D	464:465	2D	464:465	Moreover, traditional two-dimensional (2D) culture models used in cancer research do not recapitulate aspects of the 3D tumor microenvironment.
33214583	3	82	theme	traditional	435:445	arg1	models					476:481	traditional two-dimensional (2D) culture models	435:481	traditional two-dimensional (2D) culture models used in cancer research	435:505	Moreover, traditional two-dimensional (2D) culture models used in cancer research do not recapitulate aspects of the 3D tumor microenvironment.
33214583	5	83	used	used	1121:1124	arg2	we					1118:1119	we	1118:1119	we	1118:1119	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33214583	0	84	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro modeling of solid tumor interactions with perfused blood vessels.
33214583	5	85	dep	ECM	1045:1047	arg1	Matrigel					1060:1067	collagen, Matrigel, and fibrin gels	1050:1084	Matrigel	1060:1067	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33214583	5	85	dep	ECM	1045:1047	arg1	gels					1081:1084	collagen, Matrigel, and fibrin gels	1050:1084	gels	1081:1084	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33214583	4	86	theme	embedded	789:796	arg1	spheroids					779:787	tumor spheroids	773:787	tumor spheroids embedded in an extracellular matrix (ECM)	773:829	In the present study, we introduce a microfluidic model of the solid tumor-vascular interface composed of a human umbilical vein endothelial cell (HUVEC)-lined, perfusable, bioengineered blood vessel and tumor spheroids embedded in an extracellular matrix (ECM).
33214583	5	87	theme	lung	983:986	arg1	HLFs					1001:1004	HLFs	1001:1004	HLFs	1001:1004	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33214583	5	87	theme	lung	983:986	arg1	fibroblasts					988:998	/human lung fibroblasts	976:998	MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs	915:1012	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33214583	5	88	dep	matrix	1037:1042	arg1	ECM					1045:1047	ECM	1045:1047	ECM: collagen, Matrigel, and fibrin gels with or without free HLFs	1045:1110	We sought to optimize our model by varying the composition of the tumor spheroids (MDA-MB-231 breast tumor cells + mesenchymal stem cells (MSCs)/human lung fibroblasts (HLFs)/HUVECs) and the extracellular matrix (ECM: collagen, Matrigel, and fibrin gels with or without free HLFs) that we used.
33693938	3	0	theme	nitrogen	705:712	arg1	accumulation					670:681	the accumulation	666:681	the accumulation of central carbon and nitrogen metabolic intermediates	666:736	In this study, we assessed maternal nutrient supply via measurement of seed coat exudates and metabolite levels within the cotyledon throughout development to identify trends in the accumulation of central carbon and nitrogen metabolic intermediates.
33693938	5	1	theme	oligosaccharide	1358:1372	arg1	biosynthesis					1374:1385	(5) oligosaccharide biosynthesis	1354:1385	(5) oligosaccharide biosynthesis within the seed coat	1354:1406	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	6	2	theme	engineering	1470:1480	arg1	targets					1482:1488	temporal engineering targets	1461:1488	temporal engineering targets for altering final biomass composition to increase the value of soybeans and a path to breaking the inverse correlation between seed protein and oil content	1461:1645	These results highlight temporal engineering targets for altering final biomass composition to increase the value of soybeans and a path to breaking the inverse correlation between seed protein and oil content.
33693938	0	3	from	changes	9:15	arg1	metabolism					20:29	metabolism	20:29	metabolism	20:29	Temporal changes in metabolism late in seed development affect biomass composition.
33693938	0	3	from	changes	9:15	arg1	development					44:54	seed development	39:54	seed development	39:54	Temporal changes in metabolism late in seed development affect biomass composition.
33693938	1	4	theme	Glycine	156:162	arg1	soybean					147:153	soybean	147:153	soybean (Glycine max) seed	147:172	The negative association between protein and oil production in soybean (Glycine max) seed is well-documented.
33693938	1	4	theme	Glycine	156:162	arg1	max					164:166	Glycine max	156:166	Glycine max	156:166	The negative association between protein and oil production in soybean (Glycine max) seed is well-documented.
33693938	5	5	theme	gluconeogenic	1246:1258	arg1	steps					1260:1264	gluconeogenic steps	1246:1264	gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes	1246:1347	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	4	6	with	labeling	823:830	arg1	substrates					841:850	13C substrates	837:850	13C substrates	837:850	Active metabolic activity during late seed development was probed through transient labeling with 13C substrates.
33693938	3	7	theme	carbon	694:699	arg1	accumulation					670:681	the accumulation	666:681	the accumulation of central carbon and nitrogen metabolic intermediates	666:736	In this study, we assessed maternal nutrient supply via measurement of seed coat exudates and metabolite levels within the cotyledon throughout development to identify trends in the accumulation of central carbon and nitrogen metabolic intermediates.
33693938	3	8	theme	exudates	569:576	arg1	measurement					544:554	measurement	544:554	measurement of seed coat exudates and metabolite levels within the cotyledon throughout development to identify trends in the accumulation of central carbon and nitrogen metabolic intermediates	544:736	In this study, we assessed maternal nutrient supply via measurement of seed coat exudates and metabolite levels within the cotyledon throughout development to identify trends in the accumulation of central carbon and nitrogen metabolic intermediates.
33693938	2	9	theme	seed	277:280	arg1	composition					255:265	the composition	251:265	the composition	251:265	However, this inverse relationship is based primarily on the composition of mature seed, which reflects the cumulative result of events over the course of soybean seed development and therefore does not convey information specific to metabolic fluctuations during developmental growth regimes.
33693938	5	10	theme	concomitant	935:945	arg1	increase					947:954	a concomitant increase	933:954	a concomitant increase in carbohydrates	933:971	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	3	11	theme	central	686:692	arg1	carbon					694:699	central carbon	686:699	central carbon	686:699	In this study, we assessed maternal nutrient supply via measurement of seed coat exudates and metabolite levels within the cotyledon throughout development to identify trends in the accumulation of central carbon and nitrogen metabolic intermediates.
33693938	4	12	theme	seed	777:780	arg1	development					782:792	late seed development	772:792	late seed development	772:792	Active metabolic activity during late seed development was probed through transient labeling with 13C substrates.
33693938	5	13	from	changes	1071:1077	arg1	release					1101:1107	CO2 release	1097:1107	CO2 release	1097:1107	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	5	13	from	changes	1071:1077	arg1	changes					1114:1120	(3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase	1110:1434	(3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase	1110:1434	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	5	13	from	changes	1071:1077	arg1	use					1089:1091	carbon use	1082:1091	carbon use	1082:1091	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	4	14	theme	metabolic	746:754	arg1	activity					756:763	Active metabolic activity	739:763	Active metabolic activity during late seed development	739:792	Active metabolic activity during late seed development was probed through transient labeling with 13C substrates.
33693938	3	15	theme	maternal	515:522	arg1	supply					533:538	maternal nutrient supply	515:538	maternal nutrient supply	515:538	In this study, we assessed maternal nutrient supply via measurement of seed coat exudates and metabolite levels within the cotyledon throughout development to identify trends in the accumulation of central carbon and nitrogen metabolic intermediates.
33693938	5	16	dep	filling	1001:1007	arg1	2					975:975	2	975:975	2	975:975	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	3	17	from	trends	656:661	arg1	accumulation					670:681	the accumulation	666:681	the accumulation of central carbon and nitrogen metabolic intermediates	666:736	In this study, we assessed maternal nutrient supply via measurement of seed coat exudates and metabolite levels within the cotyledon throughout development to identify trends in the accumulation of central carbon and nitrogen metabolic intermediates.
33693938	3	18	theme	metabolic	714:722	arg1	intermediates					724:736	metabolic intermediates	714:736	metabolic intermediates	714:736	In this study, we assessed maternal nutrient supply via measurement of seed coat exudates and metabolite levels within the cotyledon throughout development to identify trends in the accumulation of central carbon and nitrogen metabolic intermediates.
33693938	6	19	theme	oil	1635:1637	arg1	content					1639:1645	seed protein and oil content	1618:1645	content	1639:1645	These results highlight temporal engineering targets for altering final biomass composition to increase the value of soybeans and a path to breaking the inverse correlation between seed protein and oil content.
33693938	3	20	theme	nutrient	524:531	arg1	supply					533:538	maternal nutrient supply	515:538	maternal nutrient supply	515:538	In this study, we assessed maternal nutrient supply via measurement of seed coat exudates and metabolite levels within the cotyledon throughout development to identify trends in the accumulation of central carbon and nitrogen metabolic intermediates.
33693938	2	21	theme	development	362:372	arg1	course					339:344	the course	335:344	the course of soybean seed development	335:372	However, this inverse relationship is based primarily on the composition of mature seed, which reflects the cumulative result of events over the course of soybean seed development and therefore does not convey information specific to metabolic fluctuations during developmental growth regimes.
33693938	2	22	theme	metabolic	428:436	arg1	fluctuations					438:449	metabolic fluctuations	428:449	metabolic fluctuations during developmental growth regimes	428:485	However, this inverse relationship is based primarily on the composition of mature seed, which reflects the cumulative result of events over the course of soybean seed development and therefore does not convey information specific to metabolic fluctuations during developmental growth regimes.
33693938	6	23	theme	final	1503:1507	arg1	composition					1517:1527	final biomass composition	1503:1527	final biomass composition	1503:1527	These results highlight temporal engineering targets for altering final biomass composition to increase the value of soybeans and a path to breaking the inverse correlation between seed protein and oil content.
33693938	6	24	dep	value	1545:1549	arg1	correlation					1598:1608	the inverse correlation	1586:1608	the inverse correlation between seed protein and oil content	1586:1645	These results highlight temporal engineering targets for altering final biomass composition to increase the value of soybeans and a path to breaking the inverse correlation between seed protein and oil content.
33693938	3	25	theme	seed	559:562	arg1	exudates					569:576	seed coat exudates	559:576	seed coat exudates	559:576	In this study, we assessed maternal nutrient supply via measurement of seed coat exudates and metabolite levels within the cotyledon throughout development to identify trends in the accumulation of central carbon and nitrogen metabolic intermediates.
33693938	5	26	theme	CO2	1097:1099	arg1	release					1101:1107	CO2 release	1097:1107	CO2 release	1097:1107	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	2	27	theme	seed	357:360	arg1	development					362:372	soybean seed development	349:372	soybean seed development	349:372	However, this inverse relationship is based primarily on the composition of mature seed, which reflects the cumulative result of events over the course of soybean seed development and therefore does not convey information specific to metabolic fluctuations during developmental growth regimes.
33693938	0	28	theme	Temporal	0:7	arg1	changes					9:15	Temporal changes	0:15	Temporal changes in metabolism late in seed development	0:54	Temporal changes in metabolism late in seed development affect biomass composition.
33693938	5	29	theme	nitrogen	1145:1152	arg1	resources					1154:1162	measured carbon and nitrogen resources	1125:1162	resources	1154:1162	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	5	30	dep	production	1227:1236	arg1	4					1209:1209	4	1209:1209	4	1209:1209	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	1	31	theme	negative	88:95	arg1	association					97:107	The negative association	84:107	The negative association between protein and oil production in soybean (Glycine max) seed	84:172	The negative association between protein and oil production in soybean (Glycine max) seed is well-documented.
33693938	1	31	theme	negative	88:95	arg1	well-documented					177:191	well-documented	177:191	well-documented	177:191	The negative association between protein and oil production in soybean (Glycine max) seed is well-documented.
33693938	2	32	theme	soybean	349:355	arg1	development					362:372	soybean seed development	349:372	soybean seed development	349:372	However, this inverse relationship is based primarily on the composition of mature seed, which reflects the cumulative result of events over the course of soybean seed development and therefore does not convey information specific to metabolic fluctuations during developmental growth regimes.
33693938	2	33	theme	specific	416:423	arg1	information					404:414	information	404:414	information specific to metabolic fluctuations during developmental growth regimes	404:485	However, this inverse relationship is based primarily on the composition of mature seed, which reflects the cumulative result of events over the course of soybean seed development and therefore does not convey information specific to metabolic fluctuations during developmental growth regimes.
33693938	3	34	dep	carbon	694:699	arg1	intermediates					724:736	metabolic intermediates	714:736	metabolic intermediates	714:736	In this study, we assessed maternal nutrient supply via measurement of seed coat exudates and metabolite levels within the cotyledon throughout development to identify trends in the accumulation of central carbon and nitrogen metabolic intermediates.
33693938	5	35	theme	maturation	1419:1428	arg1	phase					1430:1434	the maturation phase	1415:1434	the maturation phase	1415:1434	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	4	36	theme	late	772:775	arg1	development					782:792	late seed development	772:792	late seed development	772:792	Active metabolic activity during late seed development was probed through transient labeling with 13C substrates.
33693938	6	37	theme	seed	1618:1621	arg1	protein					1623:1629	seed protein and oil content	1618:1645	protein	1623:1629	These results highlight temporal engineering targets for altering final biomass composition to increase the value of soybeans and a path to breaking the inverse correlation between seed protein and oil content.
33693938	3	38	theme	coat	564:567	arg1	exudates					569:576	seed coat exudates	559:576	seed coat exudates	559:576	In this study, we assessed maternal nutrient supply via measurement of seed coat exudates and metabolite levels within the cotyledon throughout development to identify trends in the accumulation of central carbon and nitrogen metabolic intermediates.
33693938	6	39	theme	inverse	1590:1596	arg1	correlation					1598:1608	the inverse correlation	1586:1608	the inverse correlation between seed protein and oil content	1586:1645	These results highlight temporal engineering targets for altering final biomass composition to increase the value of soybeans and a path to breaking the inverse correlation between seed protein and oil content.
33693938	5	40	dep	drop	882:885	arg1	1					877:877	1	877:877	1	877:877	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	5	41	theme	maturation	1012:1021	arg1	phases					1023:1028	maturation phases	1012:1028	maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase	1012:1434	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	2	42	theme	growth	472:477	arg1	regimes					479:485	developmental growth regimes	458:485	developmental growth regimes	458:485	However, this inverse relationship is based primarily on the composition of mature seed, which reflects the cumulative result of events over the course of soybean seed development and therefore does not convey information specific to metabolic fluctuations during developmental growth regimes.
33693938	6	43	theme	soybeans	1554:1561	arg1	path					1569:1572	a path	1567:1572	a path to breaking	1567:1584	These results highlight temporal engineering targets for altering final biomass composition to increase the value of soybeans and a path to breaking the inverse correlation between seed protein and oil content.
33693938	6	43	theme	soybeans	1554:1561	arg1	value					1545:1549	the value	1541:1549	the value of soybeans	1541:1561	These results highlight temporal engineering targets for altering final biomass composition to increase the value of soybeans and a path to breaking the inverse correlation between seed protein and oil content.
33693938	6	44	theme	temporal	1461:1468	arg1	targets					1482:1488	temporal engineering targets	1461:1488	temporal engineering targets for altering final biomass composition to increase the value of soybeans and a path to breaking the inverse correlation between seed protein and oil content	1461:1645	These results highlight temporal engineering targets for altering final biomass composition to increase the value of soybeans and a path to breaking the inverse correlation between seed protein and oil content.
33693938	3	45	theme	metabolite	582:591	arg1	levels					593:598	metabolite levels	582:598	metabolite levels	582:598	In this study, we assessed maternal nutrient supply via measurement of seed coat exudates and metabolite levels within the cotyledon throughout development to identify trends in the accumulation of central carbon and nitrogen metabolic intermediates.
33693938	5	46	theme	nutrient	1322:1329	arg1	supply					1331:1336	the maternal nutrient supply diminishes	1309:1347	the maternal nutrient supply diminishes	1309:1347	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	2	47	theme	developmental	458:470	arg1	regimes					479:485	developmental growth regimes	458:485	developmental growth regimes	458:485	However, this inverse relationship is based primarily on the composition of mature seed, which reflects the cumulative result of events over the course of soybean seed development and therefore does not convey information specific to metabolic fluctuations during developmental growth regimes.
33693938	5	48	from	changes	1114:1120	arg1	carbon					1134:1139	measured carbon and nitrogen resources	1125:1162	carbon	1134:1139	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	5	48	from	changes	1114:1120	arg1	resources					1154:1162	measured carbon and nitrogen resources	1125:1162	resources	1154:1162	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	5	49	theme	carbon	1082:1087	arg1	changes					1114:1120	(3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase	1110:1434	(3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase	1110:1434	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	5	49	theme	carbon	1082:1087	arg1	use					1089:1091	carbon use	1082:1091	carbon use	1082:1091	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	3	50	theme	levels	593:598	arg1	measurement					544:554	measurement	544:554	measurement of seed coat exudates and metabolite levels within the cotyledon throughout development to identify trends in the accumulation of central carbon and nitrogen metabolic intermediates	544:736	In this study, we assessed maternal nutrient supply via measurement of seed coat exudates and metabolite levels within the cotyledon throughout development to identify trends in the accumulation of central carbon and nitrogen metabolic intermediates.
33693938	5	51	theme	measured	1125:1132	arg1	carbon					1134:1139	measured carbon and nitrogen resources	1125:1162	carbon	1134:1139	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	6	52	theme	biomass	1509:1515	arg1	composition					1517:1527	final biomass composition	1503:1527	final biomass composition	1503:1527	These results highlight temporal engineering targets for altering final biomass composition to increase the value of soybeans and a path to breaking the inverse correlation between seed protein and oil content.
33693938	5	53	dep	changes	1114:1120	arg1	3					1111:1111	3	1111:1111	3	1111:1111	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	2	54	theme	inverse	208:214	arg1	relationship					216:227	this inverse relationship	203:227	this inverse relationship	203:227	However, this inverse relationship is based primarily on the composition of mature seed, which reflects the cumulative result of events over the course of soybean seed development and therefore does not convey information specific to metabolic fluctuations during developmental growth regimes.
33693938	2	55	theme	events	323:328	arg1	result					313:318	the cumulative result	298:318	the cumulative result of events	298:328	However, this inverse relationship is based primarily on the composition of mature seed, which reflects the cumulative result of events over the course of soybean seed development and therefore does not convey information specific to metabolic fluctuations during developmental growth regimes.
33693938	0	56	theme	seed	39:42	arg1	development					44:54	seed development	39:54	seed development	39:54	Temporal changes in metabolism late in seed development affect biomass composition.
33693938	5	57	dep	supply	1331:1336	arg1	diminishes					1338:1347	diminishes	1338:1347	diminishes	1338:1347	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	1	58	from	association	97:107	arg1	soybean					147:153	soybean	147:153	soybean (Glycine max) seed	147:172	The negative association between protein and oil production in soybean (Glycine max) seed is well-documented.
33693938	1	58	from	association	97:107	arg1	max					164:166	Glycine max	156:166	Glycine max	156:166	The negative association between protein and oil production in soybean (Glycine max) seed is well-documented.
33693938	5	59	dep	indicated	865:873	arg1	filling					1001:1007	seed filling	996:1007	seed filling	996:1007	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	4	60	theme	transient	813:821	arg1	labeling					823:830	transient labeling	813:830	transient labeling with 13C substrates	813:850	Active metabolic activity during late seed development was probed through transient labeling with 13C substrates.
33693938	4	61	theme	13C	837:839	arg1	substrates					841:850	13C substrates	837:850	13C substrates	837:850	Active metabolic activity during late seed development was probed through transient labeling with 13C substrates.
33693938	1	62	theme	oil	129:131	arg1	production					133:142	oil production	129:142	oil production	129:142	The negative association between protein and oil production in soybean (Glycine max) seed is well-documented.
33693938	5	63	theme	sustained	1270:1278	arg1	accumulation					1293:1304	sustained carbohydrate accumulation	1270:1304	sustained carbohydrate accumulation as the maternal nutrient supply diminishes	1270:1347	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	5	64	theme	seed	1398:1401	arg1	coat					1403:1406	the seed coat	1394:1406	the seed coat	1394:1406	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	5	65	theme	seed	912:915	arg1	maturation					917:926	seed maturation	912:926	seed maturation	912:926	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	2	66	theme	cumulative	302:311	arg1	result					313:318	the cumulative result	298:318	the cumulative result of events	298:328	However, this inverse relationship is based primarily on the composition of mature seed, which reflects the cumulative result of events over the course of soybean seed development and therefore does not convey information specific to metabolic fluctuations during developmental growth regimes.
33693938	5	67	theme	carbohydrate	1280:1291	arg1	accumulation					1293:1304	sustained carbohydrate accumulation	1270:1304	sustained carbohydrate accumulation as the maternal nutrient supply diminishes	1270:1347	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	4	68	theme	Active	739:744	arg1	activity					756:763	Active metabolic activity	739:763	Active metabolic activity during late seed development	739:792	Active metabolic activity during late seed development was probed through transient labeling with 13C substrates.
33693938	0	69	theme	biomass	63:69	arg1	composition					71:81	biomass composition	63:81	biomass composition	63:81	Temporal changes in metabolism late in seed development affect biomass composition.
33693938	5	70	theme	balanced	1062:1069	arg1	changes					1071:1077	quantitatively balanced changes	1047:1077	quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase	1047:1434	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	5	71	from	drop	882:885	arg1	contents					896:903	lipid contents	890:903	lipid contents	890:903	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	5	72	from	increase	947:954	arg1	carbohydrates					959:971	carbohydrates	959:971	carbohydrates	959:971	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	5	73	theme	13C	1212:1214	arg1	production					1227:1236	(4) 13C metabolite production	1208:1236	(4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes	1208:1347	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	5	74	theme	seed	996:999	arg1	filling					1001:1007	seed filling	996:1007	seed filling	996:1007	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	5	75	theme	maternal	1313:1320	arg1	supply					1331:1336	the maternal nutrient supply diminishes	1309:1347	the maternal nutrient supply diminishes	1309:1347	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	5	76	theme	lipid	890:894	arg1	contents					896:903	lipid contents	890:903	lipid contents	890:903	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33693938	5	77	theme	metabolite	1216:1225	arg1	production					1227:1236	(4) 13C metabolite production	1208:1236	(4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes	1208:1347	The results indicated: (1) a drop in lipid contents during seed maturation with a concomitant increase in carbohydrates, (2) a transition from seed filling to maturation phases characterized by quantitatively balanced changes in carbon use and CO2 release, (3) changes in measured carbon and nitrogen resources supplied maternally throughout development, (4) 13C metabolite production through gluconeogenic steps for sustained carbohydrate accumulation as the maternal nutrient supply diminishes, and (5) oligosaccharide biosynthesis within the seed coat during the maturation phase.
33218095	4	0	theme	mannuronan	920:929	arg1	epimerases					935:944	The mannuronan C-5 epimerases	916:944	The mannuronan C-5 epimerases used in this study	916:963	The mannuronan C-5 epimerases used in this study increased the content of guluronate from 32% up to 81% in both the harvested seaweed and bacterial fermented alginate sources.
33218095	4	1	theme	alginate	1074:1081	arg1	sources					1083:1089	fermented alginate sources	1064:1089	fermented alginate sources	1064:1089	The mannuronan C-5 epimerases used in this study increased the content of guluronate from 32% up to 81% in both the harvested seaweed and bacterial fermented alginate sources.
33218095	8	2	theme	low-molecular-weight	2012:2031	arg1	alginates					2033:2041	novel low-molecular-weight alginates	2006:2041	novel low-molecular-weight alginates	2006:2041	These findings clearly illustrate the value of using epimerases to provide an alternative production route for novel low-molecular-weight alginates.
33218095	3	3	theme	guluronate	555:564	arg1	alginates					587:595	high guluronate low-molecular-weight alginates	550:595	high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525	550:913	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	6	4	theme	alginate	1663:1670	arg1	CF-5/20					1679:1685	the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20	1596:1685	the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20 as a comparator	1596:1701	Functional identity was determined by minimum inhibitory concentration (MIC) assays with selected bacteria and antibiotics using the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20 as a comparator.
33218095	5	5	theme	1H	1264:1265	arg1	resonance					1253:1261	proton nuclear magnetic resonance	1229:1261	proton nuclear magnetic resonance (1H NMR)	1229:1270	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	5	5	theme	1H	1264:1265	arg1	NMR					1267:1269	1H NMR	1264:1269	1H NMR	1264:1269	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	3	6	dep	algae	689:693	arg1	hyperborea					748:757	Laminaria hyperborea	738:757	Laminaria hyperborea	738:757	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	3	6	dep	algae	689:693	arg1	nigrescens					772:781	Lessonia nigrescens	763:781	Lessonia nigrescens	763:781	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	3	6	dep	algae	689:693	arg1	Durvillea					717:725	Durvillea	717:725	Durvillea	717:725	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	3	6	dep	algae	689:693	arg1	nodosum					708:714	Ascophyllum nodosum	696:714	Ascophyllum nodosum	696:714	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	6	7	theme	guluronate	1643:1652	arg1	CF-5/20					1679:1685	the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20	1596:1685	the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20 as a comparator	1596:1701	Functional identity was determined by minimum inhibitory concentration (MIC) assays with selected bacteria and antibiotics using the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20 as a comparator.
33218095	6	8	with	assays	1544:1549	arg1	bacteria					1565:1572	selected bacteria	1556:1572	selected bacteria	1556:1572	Functional identity was determined by minimum inhibitory concentration (MIC) assays with selected bacteria and antibiotics using the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20 as a comparator.
33218095	6	8	with	assays	1544:1549	arg1	antibiotics					1578:1588	antibiotics	1578:1588	antibiotics	1578:1588	Functional identity was determined by minimum inhibitory concentration (MIC) assays with selected bacteria and antibiotics using the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20 as a comparator.
33218095	6	9	theme	documented	1611:1620	arg1	CF-5/20					1679:1685	the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20	1596:1685	the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20 as a comparator	1596:1701	Functional identity was determined by minimum inhibitory concentration (MIC) assays with selected bacteria and antibiotics using the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20 as a comparator.
33218095	7	10	theme	potentiation	1773:1784	arg1	effects					1786:1792	similar antibiotic potentiation effects	1754:1792	similar antibiotic potentiation effects to the drug candidate OligoG CF-5/20	1754:1829	The alginates produced using either source showed similar antibiotic potentiation effects to the drug candidate OligoG CF-5/20 currently in development as a mucolytic and anti-biofilm agent.
33218095	7	10	theme	potentiation	1773:1784	arg1	agent					1888:1892	a mucolytic and anti-biofilm agent	1859:1892	agent	1888:1892	The alginates produced using either source showed similar antibiotic potentiation effects to the drug candidate OligoG CF-5/20 currently in development as a mucolytic and anti-biofilm agent.
33218095	3	11	theme	alginates	587:595	arg1	production					536:545	the production	532:545	the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525	532:913	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	7	12	theme	similar	1754:1760	arg1	effects					1786:1792	similar antibiotic potentiation effects	1754:1792	similar antibiotic potentiation effects to the drug candidate OligoG CF-5/20	1754:1829	The alginates produced using either source showed similar antibiotic potentiation effects to the drug candidate OligoG CF-5/20 currently in development as a mucolytic and anti-biofilm agent.
33218095	7	12	theme	similar	1754:1760	arg1	agent					1888:1892	a mucolytic and anti-biofilm agent	1859:1892	agent	1888:1892	The alginates produced using either source showed similar antibiotic potentiation effects to the drug candidate OligoG CF-5/20 currently in development as a mucolytic and anti-biofilm agent.
33218095	7	13	theme	anti-biofilm	1875:1886	arg1	effects					1786:1792	similar antibiotic potentiation effects	1754:1792	similar antibiotic potentiation effects to the drug candidate OligoG CF-5/20	1754:1829	The alginates produced using either source showed similar antibiotic potentiation effects to the drug candidate OligoG CF-5/20 currently in development as a mucolytic and anti-biofilm agent.
33218095	7	13	theme	anti-biofilm	1875:1886	arg1	agent					1888:1892	a mucolytic and anti-biofilm agent	1859:1892	agent	1888:1892	The alginates produced using either source showed similar antibiotic potentiation effects to the drug candidate OligoG CF-5/20 currently in development as a mucolytic and anti-biofilm agent.
33218095	3	14	theme	acid	806:809	arg1	alginate					811:818	a pure mannuronic acid alginate	788:818	a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525	788:913	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	5	15	theme	guluronate-rich	1096:1110	arg1	oligomers					1121:1129	The guluronate-rich alginate oligomers	1092:1129	The guluronate-rich alginate oligomers subsequently derived from these two different sources	1092:1183	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	3	16	theme	mannuronic	622:631	arg1	alginates					638:646	high mannuronic acid alginates	617:646	high mannuronic acid alginates	617:646	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	3	17	theme	pure	790:793	arg1	acid					806:809	a pure mannuronic acid	788:809	a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525	788:913	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	2	18	theme	alginates	222:230	arg1	composition					207:217	composition	207:217	composition	207:217	However, the content and composition of alginates differ according to the distinct parts of these macroalgae and have a direct impact on the concentration of guluronate and subsequent commercial value of the final product.
33218095	2	18	theme	alginates	222:230	arg1	content					195:201	content	195:201	content	195:201	However, the content and composition of alginates differ according to the distinct parts of these macroalgae and have a direct impact on the concentration of guluronate and subsequent commercial value of the final product.
33218095	3	19	theme	fluorescens	890:900	arg1	NCIMB					902:906	Pseudomonas fluorescens NCIMB 10,525	878:913	Pseudomonas fluorescens NCIMB 10,525	878:913	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	3	20	theme	fermented	833:841	arg1	production					843:852	fermented production	833:852	fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525	833:913	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	1	21	theme	brown	141:145	arg1	algae					147:151	marine brown algae	134:151	marine brown algae	134:151	Alginates are one of the major polysaccharide constituents of marine brown algae in commercial manufacturing.
33218095	7	22	theme	mucolytic	1861:1869	arg1	effects					1786:1792	similar antibiotic potentiation effects	1754:1792	similar antibiotic potentiation effects to the drug candidate OligoG CF-5/20	1754:1829	The alginates produced using either source showed similar antibiotic potentiation effects to the drug candidate OligoG CF-5/20 currently in development as a mucolytic and anti-biofilm agent.
33218095	7	22	theme	mucolytic	1861:1869	arg1	agent					1888:1892	a mucolytic and anti-biofilm agent	1859:1892	agent	1888:1892	The alginates produced using either source showed similar antibiotic potentiation effects to the drug candidate OligoG CF-5/20 currently in development as a mucolytic and anti-biofilm agent.
33218095	3	23	theme	NCIMB	902:906	arg1	strain					868:873	the mutant strain	857:873	the mutant strain of Pseudomonas fluorescens NCIMB 10,525	857:913	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	3	24	theme	alginates	638:646	arg1	sources					606:612	two sources	602:612	two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525	602:913	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	3	25	theme	mutant	861:866	arg1	strain					868:873	the mutant strain	857:873	the mutant strain of Pseudomonas fluorescens NCIMB 10,525	857:913	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	5	26	theme	different	1167:1175	arg1	sources					1177:1183	these two different sources	1157:1183	these two different sources	1157:1183	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	5	27	theme	pulsed	1325:1330	arg1	detection					1345:1353	pulsed amperometric detection	1325:1353	pulsed amperometric detection (HPAEC-PAD)	1325:1365	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	5	27	theme	pulsed	1325:1330	arg1	HPAEC-PAD					1356:1364	HPAEC-PAD	1356:1364	HPAEC-PAD	1356:1364	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	5	28	theme	online	1406:1411	arg1	scattering					1444:1453	online multi-angle static laser light scattering	1406:1453	online multi-angle static laser light scattering (SEC-MALS)	1406:1464	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	5	28	theme	online	1406:1411	arg1	SEC-MALS					1456:1463	SEC-MALS	1456:1463	SEC-MALS	1456:1463	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	2	29	theme	product	396:402	arg1	guluronate					340:349	guluronate	340:349	guluronate	340:349	However, the content and composition of alginates differ according to the distinct parts of these macroalgae and have a direct impact on the concentration of guluronate and subsequent commercial value of the final product.
33218095	2	29	theme	product	396:402	arg1	value					377:381	subsequent commercial value	355:381	subsequent commercial value	355:381	However, the content and composition of alginates differ according to the distinct parts of these macroalgae and have a direct impact on the concentration of guluronate and subsequent commercial value of the final product.
33218095	8	30	theme	alternative	1973:1983	arg1	route					1996:2000	an alternative production route	1970:2000	an alternative production route for novel low-molecular-weight alginates	1970:2041	These findings clearly illustrate the value of using epimerases to provide an alternative production route for novel low-molecular-weight alginates.
33218095	7	31	theme	candidate	1806:1814	arg1	CF-5/20					1823:1829	the drug candidate OligoG CF-5/20	1797:1829	the drug candidate OligoG CF-5/20	1797:1829	The alginates produced using either source showed similar antibiotic potentiation effects to the drug candidate OligoG CF-5/20 currently in development as a mucolytic and anti-biofilm agent.
33218095	5	32	theme	nuclear	1236:1242	arg1	resonance					1253:1261	proton nuclear magnetic resonance	1229:1261	proton nuclear magnetic resonance (1H NMR)	1229:1270	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	5	32	theme	nuclear	1236:1242	arg1	NMR					1267:1269	1H NMR	1264:1269	1H NMR	1264:1269	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	5	33	theme	static	1425:1430	arg1	scattering					1444:1453	online multi-angle static laser light scattering	1406:1453	online multi-angle static laser light scattering (SEC-MALS)	1406:1464	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	5	33	theme	static	1425:1430	arg1	SEC-MALS					1456:1463	SEC-MALS	1456:1463	SEC-MALS	1456:1463	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	3	34	theme	mannuronan	432:441	arg1	epimerases					447:456	The Azotobacter vinelandii mannuronan C-5 epimerases	405:456	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4	405:472	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	3	34	theme	mannuronan	432:441	arg1	AlgE4					468:472	AlgE4	468:472	AlgE4	468:472	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	3	34	theme	mannuronan	432:441	arg1	AlgE1					458:462	AlgE1	458:462	AlgE1	458:462	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	6	35	theme	concentration	1524:1536	arg1	assays					1544:1549	minimum inhibitory concentration (MIC) assays	1505:1549	minimum inhibitory concentration (MIC) assays with selected bacteria and antibiotics using the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20 as a comparator	1505:1701	Functional identity was determined by minimum inhibitory concentration (MIC) assays with selected bacteria and antibiotics using the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20 as a comparator.
33218095	3	36	attach	derived	820:826	arg2	alginate					811:818	a pure mannuronic acid alginate	788:818	a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525	788:913	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	3	36	attach	derived	820:826	arg1	production					843:852	fermented production	833:852	fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525	833:913	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	1	37	from	constituents	118:129	arg1	manufacturing					167:179	commercial manufacturing	156:179	commercial manufacturing	156:179	Alginates are one of the major polysaccharide constituents of marine brown algae in commercial manufacturing.
33218095	2	38	theme	distinct	256:263	arg1	parts					265:269	the distinct parts	252:269	the distinct parts of these macroalgae	252:289	However, the content and composition of alginates differ according to the distinct parts of these macroalgae and have a direct impact on the concentration of guluronate and subsequent commercial value of the final product.
33218095	3	39	used	used	479:482	arg2	AlgE4					468:472	AlgE4	468:472	AlgE4	468:472	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	3	39	used	used	479:482	arg2	AlgE1					458:462	AlgE1	458:462	AlgE1	458:462	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	3	39	used	used	479:482	arg2	epimerases					447:456	The Azotobacter vinelandii mannuronan C-5 epimerases	405:456	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4	405:472	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	0	40	theme	C-5	22:24	arg1	Epimerases					26:35	Mannuronan C-5 Epimerases	11:35	Mannuronan C-5 Epimerases in Commercial Alginate Production	11:69	Exploiting Mannuronan C-5 Epimerases in Commercial Alginate Production.
33218095	5	41	theme	proton	1229:1234	arg1	resonance					1253:1261	proton nuclear magnetic resonance	1229:1261	proton nuclear magnetic resonance (1H NMR)	1229:1270	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	5	41	theme	proton	1229:1234	arg1	NMR					1267:1269	1H NMR	1264:1269	1H NMR	1264:1269	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	1	42	theme	polysaccharide	103:116	arg1	constituents					118:129	the major polysaccharide constituents	93:129	the major polysaccharide constituents of marine brown algae in commercial manufacturing	93:179	Alginates are one of the major polysaccharide constituents of marine brown algae in commercial manufacturing.
33218095	3	43	theme	Azotobacter	409:419	arg1	epimerases					447:456	The Azotobacter vinelandii mannuronan C-5 epimerases	405:456	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4	405:472	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	3	43	theme	Azotobacter	409:419	arg1	AlgE4					468:472	AlgE4	468:472	AlgE4	468:472	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	3	43	theme	Azotobacter	409:419	arg1	AlgE1					458:462	AlgE1	458:462	AlgE1	458:462	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	3	44	theme	brown	683:687	arg1	algae					689:693	the naturally occurring harvested brown algae	649:693	the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens)	649:782	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	4	45	theme	81	1016:1017	arg1	%					1008:1008	%	1008:1008	%	1008:1008	The mannuronan C-5 epimerases used in this study increased the content of guluronate from 32% up to 81% in both the harvested seaweed and bacterial fermented alginate sources.
33218095	3	46	theme	occurring	663:671	arg1	algae					689:693	the naturally occurring harvested brown algae	649:693	the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens)	649:782	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	2	47	theme	guluronate	340:349	arg1	concentration					323:335	the concentration	319:335	the concentration of guluronate and subsequent commercial value of the final product	319:402	However, the content and composition of alginates differ according to the distinct parts of these macroalgae and have a direct impact on the concentration of guluronate and subsequent commercial value of the final product.
33218095	0	48	theme	Alginate	51:58	arg1	Production					60:69	Commercial Alginate Production	40:69	Commercial Alginate Production	40:69	Exploiting Mannuronan C-5 Epimerases in Commercial Alginate Production.
33218095	4	49	from	%	1018:1018	arg1	bacterial					1054:1062	bacterial	1054:1062	bacterial	1054:1062	The mannuronan C-5 epimerases used in this study increased the content of guluronate from 32% up to 81% in both the harvested seaweed and bacterial fermented alginate sources.
33218095	4	49	from	%	1018:1018	arg1	seaweed					1042:1048	seaweed	1042:1048	seaweed	1042:1048	The mannuronan C-5 epimerases used in this study increased the content of guluronate from 32% up to 81% in both the harvested seaweed and bacterial fermented alginate sources.
33218095	4	50	dep	seaweed	1042:1048	arg1	sources					1083:1089	fermented alginate sources	1064:1089	fermented alginate sources	1064:1089	The mannuronan C-5 epimerases used in this study increased the content of guluronate from 32% up to 81% in both the harvested seaweed and bacterial fermented alginate sources.
33218095	5	51	with	resonance	1253:1261	arg1	scattering					1444:1453	online multi-angle static laser light scattering	1406:1453	online multi-angle static laser light scattering (SEC-MALS)	1406:1464	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	5	51	with	resonance	1253:1261	arg1	detection					1345:1353	pulsed amperometric detection	1325:1353	pulsed amperometric detection (HPAEC-PAD)	1325:1365	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	5	51	with	resonance	1253:1261	arg1	SEC-MALS					1456:1463	SEC-MALS	1456:1463	SEC-MALS	1456:1463	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	5	51	with	resonance	1253:1261	arg1	HPAEC-PAD					1356:1364	HPAEC-PAD	1356:1364	HPAEC-PAD	1356:1364	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	2	52	dep	content	195:201	arg1	the					191:193	the	191:193	the	191:193	However, the content and composition of alginates differ according to the distinct parts of these macroalgae and have a direct impact on the concentration of guluronate and subsequent commercial value of the final product.
33218095	5	53	theme	light	1438:1442	arg1	scattering					1444:1453	online multi-angle static laser light scattering	1406:1453	online multi-angle static laser light scattering (SEC-MALS)	1406:1464	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	5	53	theme	light	1438:1442	arg1	SEC-MALS					1456:1463	SEC-MALS	1456:1463	SEC-MALS	1456:1463	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	2	54	theme	value	377:381	arg1	concentration					323:335	the concentration	319:335	the concentration of guluronate and subsequent commercial value of the final product	319:402	However, the content and composition of alginates differ according to the distinct parts of these macroalgae and have a direct impact on the concentration of guluronate and subsequent commercial value of the final product.
33218095	6	55	theme	selected	1556:1563	arg1	bacteria					1565:1572	selected bacteria	1556:1572	selected bacteria	1556:1572	Functional identity was determined by minimum inhibitory concentration (MIC) assays with selected bacteria and antibiotics using the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20 as a comparator.
33218095	6	56	theme	minimum	1505:1511	arg1	MIC					1539:1541	MIC	1539:1541	MIC	1539:1541	Functional identity was determined by minimum inhibitory concentration (MIC) assays with selected bacteria and antibiotics using the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20 as a comparator.
33218095	6	56	theme	minimum	1505:1511	arg1	concentration					1524:1536	minimum inhibitory concentration	1505:1536	minimum inhibitory concentration (MIC) assays with selected bacteria and antibiotics using the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20 as a comparator	1505:1701	Functional identity was determined by minimum inhibitory concentration (MIC) assays with selected bacteria and antibiotics using the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20 as a comparator.
33218095	4	57	theme	fermented	1064:1072	arg1	sources					1083:1089	fermented alginate sources	1064:1089	fermented alginate sources	1064:1089	The mannuronan C-5 epimerases used in this study increased the content of guluronate from 32% up to 81% in both the harvested seaweed and bacterial fermented alginate sources.
33218095	8	58	theme	novel	2006:2010	arg1	alginates					2033:2041	novel low-molecular-weight alginates	2006:2041	novel low-molecular-weight alginates	2006:2041	These findings clearly illustrate the value of using epimerases to provide an alternative production route for novel low-molecular-weight alginates.
33218095	2	59	theme	subsequent	355:364	arg1	value					377:381	subsequent commercial value	355:381	subsequent commercial value	355:381	However, the content and composition of alginates differ according to the distinct parts of these macroalgae and have a direct impact on the concentration of guluronate and subsequent commercial value of the final product.
33218095	5	60	theme	magnetic	1244:1251	arg1	resonance					1253:1261	proton nuclear magnetic resonance	1229:1261	proton nuclear magnetic resonance (1H NMR)	1229:1270	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	5	60	theme	magnetic	1244:1251	arg1	NMR					1267:1269	1H NMR	1264:1269	1H NMR	1264:1269	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	5	61	attach	derived	1144:1150	arg2	oligomers					1121:1129	The guluronate-rich alginate oligomers	1092:1129	The guluronate-rich alginate oligomers subsequently derived from these two different sources	1092:1183	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	5	61	attach	derived	1144:1150	arg1	sources					1177:1183	these two different sources	1157:1183	these two different sources	1157:1183	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	3	62	theme	high	550:553	arg1	alginates					587:595	high guluronate low-molecular-weight alginates	550:595	high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525	550:913	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	2	63	theme	macroalgae	280:289	arg1	parts					265:269	the distinct parts	252:269	the distinct parts of these macroalgae	252:289	However, the content and composition of alginates differ according to the distinct parts of these macroalgae and have a direct impact on the concentration of guluronate and subsequent commercial value of the final product.
33218095	6	64	theme	enriched	1654:1661	arg1	CF-5/20					1679:1685	the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20	1596:1685	the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20 as a comparator	1596:1701	Functional identity was determined by minimum inhibitory concentration (MIC) assays with selected bacteria and antibiotics using the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20 as a comparator.
33218095	6	65	theme	low-molecular-weight	1622:1641	arg1	CF-5/20					1679:1685	the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20	1596:1685	the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20 as a comparator	1596:1701	Functional identity was determined by minimum inhibitory concentration (MIC) assays with selected bacteria and antibiotics using the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20 as a comparator.
33218095	7	66	theme	antibiotic	1762:1771	arg1	effects					1786:1792	similar antibiotic potentiation effects	1754:1792	similar antibiotic potentiation effects to the drug candidate OligoG CF-5/20	1754:1829	The alginates produced using either source showed similar antibiotic potentiation effects to the drug candidate OligoG CF-5/20 currently in development as a mucolytic and anti-biofilm agent.
33218095	7	66	theme	antibiotic	1762:1771	arg1	agent					1888:1892	a mucolytic and anti-biofilm agent	1859:1892	agent	1888:1892	The alginates produced using either source showed similar antibiotic potentiation effects to the drug candidate OligoG CF-5/20 currently in development as a mucolytic and anti-biofilm agent.
33218095	2	67	theme	direct	302:307	arg1	impact					309:314	a direct impact	300:314	a direct impact on the concentration of guluronate and subsequent commercial value of the final product	300:402	However, the content and composition of alginates differ according to the distinct parts of these macroalgae and have a direct impact on the concentration of guluronate and subsequent commercial value of the final product.
33218095	5	68	with	chromatography	1386:1399	arg1	scattering					1444:1453	online multi-angle static laser light scattering	1406:1453	online multi-angle static laser light scattering (SEC-MALS)	1406:1464	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	5	68	with	chromatography	1386:1399	arg1	detection					1345:1353	pulsed amperometric detection	1325:1353	pulsed amperometric detection (HPAEC-PAD)	1325:1365	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	5	68	with	chromatography	1386:1399	arg1	SEC-MALS					1456:1463	SEC-MALS	1456:1463	SEC-MALS	1456:1463	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	5	68	with	chromatography	1386:1399	arg1	HPAEC-PAD					1356:1364	HPAEC-PAD	1356:1364	HPAEC-PAD	1356:1364	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	3	69	theme	high	617:620	arg1	alginates					638:646	high mannuronic acid alginates	617:646	high mannuronic acid alginates	617:646	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	3	70	theme	alginate	811:818	arg1	sources					606:612	two sources	602:612	two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525	602:913	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	5	71	theme	alginate	1112:1119	arg1	oligomers					1121:1129	The guluronate-rich alginate oligomers	1092:1129	The guluronate-rich alginate oligomers subsequently derived from these two different sources	1092:1183	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	1	72	theme	constituents	118:129	arg1	constituents					118:129	the major polysaccharide constituents	93:129	the major polysaccharide constituents of marine brown algae in commercial manufacturing	93:179	Alginates are one of the major polysaccharide constituents of marine brown algae in commercial manufacturing.
33218095	1	72	theme	constituents	118:129	arg1	one					86:88	one	86:88	one	86:88	Alginates are one of the major polysaccharide constituents of marine brown algae in commercial manufacturing.
33218095	4	73	theme	C-5	931:933	arg1	epimerases					935:944	The mannuronan C-5 epimerases	916:944	The mannuronan C-5 epimerases used in this study	916:963	The mannuronan C-5 epimerases used in this study increased the content of guluronate from 32% up to 81% in both the harvested seaweed and bacterial fermented alginate sources.
33218095	5	74	theme	amperometric	1332:1343	arg1	detection					1345:1353	pulsed amperometric detection	1325:1353	pulsed amperometric detection (HPAEC-PAD)	1325:1365	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	5	74	theme	amperometric	1332:1343	arg1	HPAEC-PAD					1356:1364	HPAEC-PAD	1356:1364	HPAEC-PAD	1356:1364	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	3	75	theme	mannuronic	795:804	arg1	acid					806:809	a pure mannuronic acid	788:809	a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525	788:913	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	4	76	dep	%	1008:1008	arg1	up					1010:1011	up	1010:1011	up	1010:1011	The mannuronan C-5 epimerases used in this study increased the content of guluronate from 32% up to 81% in both the harvested seaweed and bacterial fermented alginate sources.
33218095	4	76	dep	%	1008:1008	arg1	to					1013:1014	to	1013:1014	to	1013:1014	The mannuronan C-5 epimerases used in this study increased the content of guluronate from 32% up to 81% in both the harvested seaweed and bacterial fermented alginate sources.
33218095	1	77	theme	marine	134:139	arg1	algae					147:151	marine brown algae	134:151	marine brown algae	134:151	Alginates are one of the major polysaccharide constituents of marine brown algae in commercial manufacturing.
33218095	3	78	theme	low-molecular-weight	566:585	arg1	alginates					587:595	high guluronate low-molecular-weight alginates	550:595	high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525	550:913	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	6	79	theme	Functional	1467:1476	arg1	identity					1478:1485	Functional identity	1467:1485	Functional identity	1467:1485	Functional identity was determined by minimum inhibitory concentration (MIC) assays with selected bacteria and antibiotics using the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20 as a comparator.
33218095	3	80	theme	acid	633:636	arg1	alginates					638:646	high mannuronic acid alginates	617:646	high mannuronic acid alginates	617:646	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	1	81	theme	algae	147:151	arg1	constituents					118:129	the major polysaccharide constituents	93:129	the major polysaccharide constituents of marine brown algae in commercial manufacturing	93:179	Alginates are one of the major polysaccharide constituents of marine brown algae in commercial manufacturing.
33218095	3	82	theme	Pseudomonas	878:888	arg1	NCIMB					902:906	Pseudomonas fluorescens NCIMB 10,525	878:913	Pseudomonas fluorescens NCIMB 10,525	878:913	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	4	83	theme	32	1006:1007	arg1	%					1008:1008	%	1008:1008	%	1008:1008	The mannuronan C-5 epimerases used in this study increased the content of guluronate from 32% up to 81% in both the harvested seaweed and bacterial fermented alginate sources.
33218095	0	84	from	Epimerases	26:35	arg1	Production					60:69	Commercial Alginate Production	40:69	Commercial Alginate Production	40:69	Exploiting Mannuronan C-5 Epimerases in Commercial Alginate Production.
33218095	5	85	theme	anion-exchange	1290:1303	arg1	chromatography					1305:1318	high-performance anion-exchange chromatography	1273:1318	high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD)	1273:1365	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	6	86	theme	OligoG	1672:1677	arg1	CF-5/20					1679:1685	the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20	1596:1685	the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20 as a comparator	1596:1701	Functional identity was determined by minimum inhibitory concentration (MIC) assays with selected bacteria and antibiotics using the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20 as a comparator.
33218095	1	87	theme	commercial	156:165	arg1	manufacturing					167:179	commercial manufacturing	156:179	commercial manufacturing	156:179	Alginates are one of the major polysaccharide constituents of marine brown algae in commercial manufacturing.
33218095	3	88	from	production	536:545	arg1	sources					606:612	two sources	602:612	two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525	602:913	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	3	89	theme	strain	868:873	arg1	production					843:852	fermented production	833:852	fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525	833:913	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	5	90	with	chromatography	1305:1318	arg1	scattering					1444:1453	online multi-angle static laser light scattering	1406:1453	online multi-angle static laser light scattering (SEC-MALS)	1406:1464	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	5	90	with	chromatography	1305:1318	arg1	detection					1345:1353	pulsed amperometric detection	1325:1353	pulsed amperometric detection (HPAEC-PAD)	1325:1365	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	5	90	with	chromatography	1305:1318	arg1	SEC-MALS					1456:1463	SEC-MALS	1456:1463	SEC-MALS	1456:1463	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	5	90	with	chromatography	1305:1318	arg1	HPAEC-PAD					1356:1364	HPAEC-PAD	1356:1364	HPAEC-PAD	1356:1364	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	2	91	from	impact	309:314	arg1	concentration					323:335	the concentration	319:335	the concentration of guluronate and subsequent commercial value of the final product	319:402	However, the content and composition of alginates differ according to the distinct parts of these macroalgae and have a direct impact on the concentration of guluronate and subsequent commercial value of the final product.
33218095	4	92	theme	guluronate	990:999	arg1	content					979:985	the content	975:985	the content of guluronate	975:999	The mannuronan C-5 epimerases used in this study increased the content of guluronate from 32% up to 81% in both the harvested seaweed and bacterial fermented alginate sources.
33218095	5	93	theme	multi-angle	1413:1423	arg1	scattering					1444:1453	online multi-angle static laser light scattering	1406:1453	online multi-angle static laser light scattering (SEC-MALS)	1406:1464	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	5	93	theme	multi-angle	1413:1423	arg1	SEC-MALS					1456:1463	SEC-MALS	1456:1463	SEC-MALS	1456:1463	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	2	94	theme	final	390:394	arg1	product					396:402	the final product	386:402	the final product	386:402	However, the content and composition of alginates differ according to the distinct parts of these macroalgae and have a direct impact on the concentration of guluronate and subsequent commercial value of the final product.
33218095	0	95	theme	Mannuronan	11:20	arg1	Epimerases					26:35	Mannuronan C-5 Epimerases	11:35	Mannuronan C-5 Epimerases in Commercial Alginate Production	11:69	Exploiting Mannuronan C-5 Epimerases in Commercial Alginate Production.
33218095	7	96	theme	OligoG	1816:1821	arg1	CF-5/20					1823:1829	the drug candidate OligoG CF-5/20	1797:1829	the drug candidate OligoG CF-5/20	1797:1829	The alginates produced using either source showed similar antibiotic potentiation effects to the drug candidate OligoG CF-5/20 currently in development as a mucolytic and anti-biofilm agent.
33218095	3	97	from	sources	606:612	arg1	alginates					587:595	high guluronate low-molecular-weight alginates	550:595	high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525	550:913	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	3	97	from	sources	606:612	arg1	production					536:545	the production	532:545	the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525	532:913	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	3	98	theme	C-5	443:445	arg1	epimerases					447:456	The Azotobacter vinelandii mannuronan C-5 epimerases	405:456	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4	405:472	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	3	98	theme	C-5	443:445	arg1	AlgE4					468:472	AlgE4	468:472	AlgE4	468:472	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	3	98	theme	C-5	443:445	arg1	AlgE1					458:462	AlgE1	458:462	AlgE1	458:462	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	6	99	theme	inhibitory	1513:1522	arg1	MIC					1539:1541	MIC	1539:1541	MIC	1539:1541	Functional identity was determined by minimum inhibitory concentration (MIC) assays with selected bacteria and antibiotics using the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20 as a comparator.
33218095	6	99	theme	inhibitory	1513:1522	arg1	concentration					1524:1536	minimum inhibitory concentration	1505:1536	minimum inhibitory concentration (MIC) assays with selected bacteria and antibiotics using the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20 as a comparator	1505:1701	Functional identity was determined by minimum inhibitory concentration (MIC) assays with selected bacteria and antibiotics using the previously documented low-molecular-weight guluronate enriched alginate OligoG CF-5/20 as a comparator.
33218095	3	100	dep	Durvillea	717:725	arg1	potatorum					727:735	Durvillea potatorum	717:735	Durvillea potatorum	717:735	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	8	101	theme	production	1985:1994	arg1	route					1996:2000	an alternative production route	1970:2000	an alternative production route for novel low-molecular-weight alginates	1970:2041	These findings clearly illustrate the value of using epimerases to provide an alternative production route for novel low-molecular-weight alginates.
33218095	1	102	theme	major	97:101	arg1	constituents					118:129	the major polysaccharide constituents	93:129	the major polysaccharide constituents of marine brown algae in commercial manufacturing	93:179	Alginates are one of the major polysaccharide constituents of marine brown algae in commercial manufacturing.
33218095	7	103	theme	drug	1801:1804	arg1	CF-5/20					1823:1829	the drug candidate OligoG CF-5/20	1797:1829	the drug candidate OligoG CF-5/20	1797:1829	The alginates produced using either source showed similar antibiotic potentiation effects to the drug candidate OligoG CF-5/20 currently in development as a mucolytic and anti-biofilm agent.
33218095	3	104	theme	harvested	673:681	arg1	algae					689:693	the naturally occurring harvested brown algae	649:693	the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens)	649:782	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	5	105	theme	size-exclusion	1371:1384	arg1	chromatography					1386:1399	size-exclusion chromatography	1371:1399	size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS)	1371:1464	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	0	106	theme	Commercial	40:49	arg1	Production					60:69	Commercial Alginate Production	40:69	Commercial Alginate Production	40:69	Exploiting Mannuronan C-5 Epimerases in Commercial Alginate Production.
33218095	2	107	contain	have	295:298	arg2	impact					309:314	a direct impact	300:314	a direct impact on the concentration of guluronate and subsequent commercial value of the final product	300:402	However, the content and composition of alginates differ according to the distinct parts of these macroalgae and have a direct impact on the concentration of guluronate and subsequent commercial value of the final product.
33218095	2	107	contain	have	295:298	arg1	content					195:201	content	195:201	content	195:201	However, the content and composition of alginates differ according to the distinct parts of these macroalgae and have a direct impact on the concentration of guluronate and subsequent commercial value of the final product.
33218095	3	108	theme	vinelandii	421:430	arg1	epimerases					447:456	The Azotobacter vinelandii mannuronan C-5 epimerases	405:456	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4	405:472	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	3	108	theme	vinelandii	421:430	arg1	AlgE4					468:472	AlgE4	468:472	AlgE4	468:472	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	3	108	theme	vinelandii	421:430	arg1	AlgE1					458:462	AlgE1	458:462	AlgE1	458:462	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	5	109	theme	structural	1192:1201	arg1	identity					1203:1210	structural identity	1192:1210	structural identity	1192:1210	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	3	110	dep	epimerases	447:456	arg1	epimerases					447:456	The Azotobacter vinelandii mannuronan C-5 epimerases	405:456	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4	405:472	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	3	110	dep	epimerases	447:456	arg1	AlgE4					468:472	AlgE4	468:472	AlgE4	468:472	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	3	110	dep	epimerases	447:456	arg1	AlgE1					458:462	AlgE1	458:462	AlgE1	458:462	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	3	111	theme	potential	503:511	arg1	value					513:517	their potential value	497:517	their potential value	497:517	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	3	112	theme	algae	689:693	arg1	sources					606:612	two sources	602:612	two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525	602:913	The Azotobacter vinelandii mannuronan C-5 epimerases AlgE1 and AlgE4 were used to determine their potential value in tailoring the production of high guluronate low-molecular-weight alginates from two sources of high mannuronic acid alginates, the naturally occurring harvested brown algae (Ascophyllum nodosum, Durvillea potatorum, Laminaria hyperborea and Lessonia nigrescens) and a pure mannuronic acid alginate derived from fermented production of the mutant strain of Pseudomonas fluorescens NCIMB 10,525.
33218095	5	113	theme	laser	1432:1436	arg1	scattering					1444:1453	online multi-angle static laser light scattering	1406:1453	online multi-angle static laser light scattering (SEC-MALS)	1406:1464	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	5	113	theme	laser	1432:1436	arg1	SEC-MALS					1456:1463	SEC-MALS	1456:1463	SEC-MALS	1456:1463	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
33218095	2	114	theme	commercial	366:375	arg1	value					377:381	subsequent commercial value	355:381	subsequent commercial value	355:381	However, the content and composition of alginates differ according to the distinct parts of these macroalgae and have a direct impact on the concentration of guluronate and subsequent commercial value of the final product.
33218095	5	115	theme	high-performance	1273:1288	arg1	chromatography					1305:1318	high-performance anion-exchange chromatography	1273:1318	high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD)	1273:1365	The guluronate-rich alginate oligomers subsequently derived from these two different sources showed structural identity as determined by proton nuclear magnetic resonance (1H NMR), high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and size-exclusion chromatography with online multi-angle static laser light scattering (SEC-MALS).
31596193	2	0	theme	typical	93:99	arg1	characteristics					115:129	typical morphological characteristics	93:129	typical morphological characteristics of the genus Micromonospora	93:157	An isolate, 13K206T, with typical morphological characteristics of the genus Micromonospora was obtained during a study searching for novel actinobacteria with biosynthetic potential from the Karakum Desert.
31596193	5	1	theme	16S	708:710	arg1	sequences					722:730	16S rRNA gene sequences	708:730	16S rRNA gene sequences	708:730	The results of phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain was closely related to 'Micromonospora spongicola' S3-1T, Micromonospora nigra DSM 43818T and Micromonospora yasonensis DS3186T with sequence similarities of 98.6, 98.5 and 98.4 %, respectively.
31596193	6	2	theme	Digital	952:958	arg1	hybridization					968:980	Digital DNA-DNA hybridization	952:980	Digital DNA-DNA hybridization	952:980	Digital DNA-DNA hybridization and average nucleotide identity analyses in addition to gyrB gene analysis confirmed the assignment of the strain to a novel species within the genus Micromonospora for which the name Micromonospora deserti sp.
31596193	4	3	theme	major	642:646	arg1	lipids					654:659	major polar lipids	642:659	major polar lipids	642:659	The strain showed chemotaxonomical properties consistent with its classification in the genus Micromonospora such as meso- and 3-OH-A2pm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids.
31596193	3	4	theme	taxonomic	322:330	arg1	affiliation					332:342	taxonomic affiliation	322:342	taxonomic affiliation of the strain	322:356	A polyphasic approach was adopted to determine taxonomic affiliation of the strain.
31596193	6	5	theme	hybridization	968:980	arg1	analyses					1014:1021	Digital DNA-DNA hybridization and average nucleotide identity analyses	952:1021	Digital DNA-DNA hybridization and average nucleotide identity analyses in addition to gyrB gene analysis	952:1055	Digital DNA-DNA hybridization and average nucleotide identity analyses in addition to gyrB gene analysis confirmed the assignment of the strain to a novel species within the genus Micromonospora for which the name Micromonospora deserti sp.
31596193	4	6	from	xylose	528:533	arg1	diphosphatidylglycerol					565:586	diphosphatidylglycerol	565:586	diphosphatidylglycerol	565:586	The strain showed chemotaxonomical properties consistent with its classification in the genus Micromonospora such as meso- and 3-OH-A2pm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids.
31596193	4	6	from	xylose	528:533	arg1	hydrolysate					549:559	whole-cell hydrolysate	538:559	whole-cell hydrolysate	538:559	The strain showed chemotaxonomical properties consistent with its classification in the genus Micromonospora such as meso- and 3-OH-A2pm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids.
31596193	4	6	from	xylose	528:533	arg1	peptidoglycan					513:525	the cell-wall peptidoglycan	499:525	the cell-wall peptidoglycan	499:525	The strain showed chemotaxonomical properties consistent with its classification in the genus Micromonospora such as meso- and 3-OH-A2pm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids.
31596193	6	7	theme	DNA-DNA	960:966	arg1	hybridization					968:980	Digital DNA-DNA hybridization	952:980	Digital DNA-DNA hybridization	952:980	Digital DNA-DNA hybridization and average nucleotide identity analyses in addition to gyrB gene analysis confirmed the assignment of the strain to a novel species within the genus Micromonospora for which the name Micromonospora deserti sp.
31596193	5	8	theme	gene	717:720	arg1	sequences					722:730	16S rRNA gene sequences	708:730	16S rRNA gene sequences	708:730	The results of phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain was closely related to 'Micromonospora spongicola' S3-1T, Micromonospora nigra DSM 43818T and Micromonospora yasonensis DS3186T with sequence similarities of 98.6, 98.5 and 98.4 %, respectively.
31596193	4	9	theme	consistent	405:414	arg1	properties					394:403	chemotaxonomical properties	377:403	chemotaxonomical properties consistent with its classification in the genus Micromonospora such as meso- and 3-OH-A2pm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids	377:659	The strain showed chemotaxonomical properties consistent with its classification in the genus Micromonospora such as meso- and 3-OH-A2pm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids.
31596193	4	10	from	classification	425:438	arg1	Micromonospora					453:466	the genus Micromonospora	443:466	the genus Micromonospora	443:466	The strain showed chemotaxonomical properties consistent with its classification in the genus Micromonospora such as meso- and 3-OH-A2pm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids.
31596193	8	11	theme	type	1215:1218	arg1	strain					1220:1225	The type strain	1211:1225	The type strain	1211:1225	The type strain is 13K206T (=JCM 32583T=DSM 107532T).
31596193	8	11	theme	type	1215:1218	arg1	13K206T					1230:1236	13K206T	1230:1236	13K206T (=JCM 32583T=DSM 107532T)	1230:1262	The type strain is 13K206T (=JCM 32583T=DSM 107532T).
31596193	2	12	theme	genus	138:142	arg1	Micromonospora					144:157	the genus Micromonospora	134:157	the genus Micromonospora	134:157	An isolate, 13K206T, with typical morphological characteristics of the genus Micromonospora was obtained during a study searching for novel actinobacteria with biosynthetic potential from the Karakum Desert.
31596193	1	13	theme	Karakum	51:57	arg1	Desert					59:64	the Karakum Desert	47:64	the Karakum Desert	47:64	nov., isolated from the Karakum Desert.
31596193	4	14	theme	polar	648:652	arg1	lipids					654:659	major polar lipids	642:659	major polar lipids	642:659	The strain showed chemotaxonomical properties consistent with its classification in the genus Micromonospora such as meso- and 3-OH-A2pm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids.
31596193	5	15	theme	phylogenetic	677:688	arg1	analysis					690:697	phylogenetic analysis	677:697	phylogenetic analysis based on 16S rRNA gene sequences	677:730	The results of phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain was closely related to 'Micromonospora spongicola' S3-1T, Micromonospora nigra DSM 43818T and Micromonospora yasonensis DS3186T with sequence similarities of 98.6, 98.5 and 98.4 %, respectively.
31596193	6	16	theme	genus	1126:1130	arg1	Micromonospora					1132:1145	the genus Micromonospora	1122:1145	the genus Micromonospora for which the name Micromonospora deserti sp	1122:1190	Digital DNA-DNA hybridization and average nucleotide identity analyses in addition to gyrB gene analysis confirmed the assignment of the strain to a novel species within the genus Micromonospora for which the name Micromonospora deserti sp.
31596193	3	17	theme	strain	351:356	arg1	affiliation					332:342	taxonomic affiliation	322:342	taxonomic affiliation of the strain	322:356	A polyphasic approach was adopted to determine taxonomic affiliation of the strain.
31596193	4	18	theme	cell-wall	503:511	arg1	peptidoglycan					513:525	the cell-wall peptidoglycan	499:525	the cell-wall peptidoglycan	499:525	The strain showed chemotaxonomical properties consistent with its classification in the genus Micromonospora such as meso- and 3-OH-A2pm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids.
31596193	5	19	theme	analysis	690:697	arg1	results					666:672	The results	662:672	The results of phylogenetic analysis based on 16S rRNA gene sequences	662:730	The results of phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain was closely related to 'Micromonospora spongicola' S3-1T, Micromonospora nigra DSM 43818T and Micromonospora yasonensis DS3186T with sequence similarities of 98.6, 98.5 and 98.4 %, respectively.
31596193	0	20	theme	deserti	15:21	arg1	sp					23:24	Micromonospora deserti sp	0:24	Micromonospora deserti sp.	0:25	Micromonospora deserti sp.
31596193	5	21	theme	nigra	830:834	arg1	43818T					840:845	nigra DSM 43818T	830:845	'Micromonospora spongicola' S3-1T, Micromonospora nigra DSM 43818T	780:845	The results of phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain was closely related to 'Micromonospora spongicola' S3-1T, Micromonospora nigra DSM 43818T and Micromonospora yasonensis DS3186T with sequence similarities of 98.6, 98.5 and 98.4 %, respectively.
31596193	6	22	theme	gene	1043:1046	arg1	analysis					1048:1055	gyrB gene analysis	1038:1055	gyrB gene analysis	1038:1055	Digital DNA-DNA hybridization and average nucleotide identity analyses in addition to gyrB gene analysis confirmed the assignment of the strain to a novel species within the genus Micromonospora for which the name Micromonospora deserti sp.
31596193	5	23	theme	98.4	930:933	arg1	%					935:935	98.6, 98.5 and 98.4 %	915:935	%	935:935	The results of phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain was closely related to 'Micromonospora spongicola' S3-1T, Micromonospora nigra DSM 43818T and Micromonospora yasonensis DS3186T with sequence similarities of 98.6, 98.5 and 98.4 %, respectively.
31596193	0	24	theme	Micromonospora	0:13	arg1	sp					23:24	Micromonospora deserti sp	0:24	Micromonospora deserti sp.	0:25	Micromonospora deserti sp.
31596193	2	25	with	actinobacteria	207:220	arg1	potential					240:248	biosynthetic potential	227:248	biosynthetic potential	227:248	An isolate, 13K206T, with typical morphological characteristics of the genus Micromonospora was obtained during a study searching for novel actinobacteria with biosynthetic potential from the Karakum Desert.
31596193	4	26	from	3-OH-A2pm	486:494	arg1	diphosphatidylglycerol					565:586	diphosphatidylglycerol	565:586	diphosphatidylglycerol	565:586	The strain showed chemotaxonomical properties consistent with its classification in the genus Micromonospora such as meso- and 3-OH-A2pm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids.
31596193	4	26	from	3-OH-A2pm	486:494	arg1	hydrolysate					549:559	whole-cell hydrolysate	538:559	whole-cell hydrolysate	538:559	The strain showed chemotaxonomical properties consistent with its classification in the genus Micromonospora such as meso- and 3-OH-A2pm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids.
31596193	4	26	from	3-OH-A2pm	486:494	arg1	peptidoglycan					513:525	the cell-wall peptidoglycan	499:525	the cell-wall peptidoglycan	499:525	The strain showed chemotaxonomical properties consistent with its classification in the genus Micromonospora such as meso- and 3-OH-A2pm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids.
31596193	5	27	theme	rRNA	712:715	arg1	sequences					722:730	16S rRNA gene sequences	708:730	16S rRNA gene sequences	708:730	The results of phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain was closely related to 'Micromonospora spongicola' S3-1T, Micromonospora nigra DSM 43818T and Micromonospora yasonensis DS3186T with sequence similarities of 98.6, 98.5 and 98.4 %, respectively.
31596193	4	28	from	phosphatidylethanolamine	589:612	arg1	diphosphatidylglycerol					565:586	diphosphatidylglycerol	565:586	diphosphatidylglycerol	565:586	The strain showed chemotaxonomical properties consistent with its classification in the genus Micromonospora such as meso- and 3-OH-A2pm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids.
31596193	4	28	from	phosphatidylethanolamine	589:612	arg1	hydrolysate					549:559	whole-cell hydrolysate	538:559	whole-cell hydrolysate	538:559	The strain showed chemotaxonomical properties consistent with its classification in the genus Micromonospora such as meso- and 3-OH-A2pm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids.
31596193	4	28	from	phosphatidylethanolamine	589:612	arg1	peptidoglycan					513:525	the cell-wall peptidoglycan	499:525	the cell-wall peptidoglycan	499:525	The strain showed chemotaxonomical properties consistent with its classification in the genus Micromonospora such as meso- and 3-OH-A2pm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids.
31596193	6	29	theme	gyrB	1038:1041	arg1	analysis					1048:1055	gyrB gene analysis	1038:1055	gyrB gene analysis	1038:1055	Digital DNA-DNA hybridization and average nucleotide identity analyses in addition to gyrB gene analysis confirmed the assignment of the strain to a novel species within the genus Micromonospora for which the name Micromonospora deserti sp.
31596193	4	30	theme	chemotaxonomical	377:392	arg1	properties					394:403	chemotaxonomical properties	377:403	chemotaxonomical properties consistent with its classification in the genus Micromonospora such as meso- and 3-OH-A2pm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids	377:659	The strain showed chemotaxonomical properties consistent with its classification in the genus Micromonospora such as meso- and 3-OH-A2pm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids.
31596193	5	31	with	related	769:775	arg1	similarities					899:910	sequence similarities	890:910	sequence similarities of 98.6, 98.5 and 98.4 %, respectively	890:949	The results of phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain was closely related to 'Micromonospora spongicola' S3-1T, Micromonospora nigra DSM 43818T and Micromonospora yasonensis DS3186T with sequence similarities of 98.6, 98.5 and 98.4 %, respectively.
31596193	4	32	with	consistent	405:414	arg1	classification					425:438	its classification	421:438	its classification in the genus Micromonospora such as meso- and 3-OH-A2pm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids	421:659	The strain showed chemotaxonomical properties consistent with its classification in the genus Micromonospora such as meso- and 3-OH-A2pm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids.
31596193	3	33	theme	polyphasic	277:286	arg1	approach					288:295	A polyphasic approach	275:295	A polyphasic approach	275:295	A polyphasic approach was adopted to determine taxonomic affiliation of the strain.
31596193	9	34	theme	G+C	1273:1275	arg1	%					1315:1315	72.4 mol%	1307:1315	72.4 mol%	1307:1315	The DNA G+C content of the type strain is 72.4 mol%.
31596193	9	34	theme	G+C	1273:1275	arg1	content					1277:1283	The DNA G+C content	1265:1283	The DNA G+C content of the type strain	1265:1302	The DNA G+C content of the type strain is 72.4 mol%.
31596193	5	35	theme	DSM	836:838	arg1	43818T					840:845	nigra DSM 43818T	830:845	'Micromonospora spongicola' S3-1T, Micromonospora nigra DSM 43818T	780:845	The results of phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain was closely related to 'Micromonospora spongicola' S3-1T, Micromonospora nigra DSM 43818T and Micromonospora yasonensis DS3186T with sequence similarities of 98.6, 98.5 and 98.4 %, respectively.
31596193	6	36	theme	novel	1101:1105	arg1	species					1107:1113	a novel species	1099:1113	a novel species within the genus Micromonospora for which the name Micromonospora deserti sp	1099:1190	Digital DNA-DNA hybridization and average nucleotide identity analyses in addition to gyrB gene analysis confirmed the assignment of the strain to a novel species within the genus Micromonospora for which the name Micromonospora deserti sp.
31596193	2	37	theme	biosynthetic	227:238	arg1	potential					240:248	biosynthetic potential	227:248	biosynthetic potential	227:248	An isolate, 13K206T, with typical morphological characteristics of the genus Micromonospora was obtained during a study searching for novel actinobacteria with biosynthetic potential from the Karakum Desert.
31596193	5	38	theme	%	935:935	arg1	similarities					899:910	sequence similarities	890:910	sequence similarities of 98.6, 98.5 and 98.4 %, respectively	890:949	The results of phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain was closely related to 'Micromonospora spongicola' S3-1T, Micromonospora nigra DSM 43818T and Micromonospora yasonensis DS3186T with sequence similarities of 98.6, 98.5 and 98.4 %, respectively.
31596193	5	39	theme	98.6	915:918	arg1	%					935:935	98.6, 98.5 and 98.4 %	915:935	%	935:935	The results of phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain was closely related to 'Micromonospora spongicola' S3-1T, Micromonospora nigra DSM 43818T and Micromonospora yasonensis DS3186T with sequence similarities of 98.6, 98.5 and 98.4 %, respectively.
31596193	8	40	theme	=JCM	1239:1242	arg1	107532T					1255:1261	=JCM 32583T=DSM 107532T	1239:1261	=JCM 32583T=DSM 107532T	1239:1261	The type strain is 13K206T (=JCM 32583T=DSM 107532T).
31596193	8	40	theme	=JCM	1239:1242	arg1	13K206T					1230:1236	13K206T	1230:1236	13K206T (=JCM 32583T=DSM 107532T)	1230:1262	The type strain is 13K206T (=JCM 32583T=DSM 107532T).
31596193	9	41	theme	72.4 mol	1307:1314	arg1	%					1315:1315	72.4 mol%	1307:1315	72.4 mol%	1307:1315	The DNA G+C content of the type strain is 72.4 mol%.
31596193	9	41	theme	72.4 mol	1307:1314	arg1	content					1277:1283	The DNA G+C content	1265:1283	The DNA G+C content of the type strain	1265:1302	The DNA G+C content of the type strain is 72.4 mol%.
31596193	4	42	theme	whole-cell	538:547	arg1	hydrolysate					549:559	whole-cell hydrolysate	538:559	whole-cell hydrolysate	538:559	The strain showed chemotaxonomical properties consistent with its classification in the genus Micromonospora such as meso- and 3-OH-A2pm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids.
31596193	5	43	dep	spongicola	796:805	arg1	Micromonospora					815:828	Micromonospora	815:828	Micromonospora	815:828	The results of phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain was closely related to 'Micromonospora spongicola' S3-1T, Micromonospora nigra DSM 43818T and Micromonospora yasonensis DS3186T with sequence similarities of 98.6, 98.5 and 98.4 %, respectively.
31596193	5	43	dep	spongicola	796:805	arg1	43818T					840:845	nigra DSM 43818T	830:845	'Micromonospora spongicola' S3-1T, Micromonospora nigra DSM 43818T	780:845	The results of phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain was closely related to 'Micromonospora spongicola' S3-1T, Micromonospora nigra DSM 43818T and Micromonospora yasonensis DS3186T with sequence similarities of 98.6, 98.5 and 98.4 %, respectively.
31596193	2	44	theme	Micromonospora	144:157	arg1	characteristics					115:129	typical morphological characteristics	93:129	typical morphological characteristics of the genus Micromonospora	93:157	An isolate, 13K206T, with typical morphological characteristics of the genus Micromonospora was obtained during a study searching for novel actinobacteria with biosynthetic potential from the Karakum Desert.
31596193	9	45	theme	DNA	1269:1271	arg1	%					1315:1315	72.4 mol%	1307:1315	72.4 mol%	1307:1315	The DNA G+C content of the type strain is 72.4 mol%.
31596193	9	45	theme	DNA	1269:1271	arg1	content					1277:1283	The DNA G+C content	1265:1283	The DNA G+C content of the type strain	1265:1302	The DNA G+C content of the type strain is 72.4 mol%.
31596193	4	46	from	meso-	476:480	arg1	diphosphatidylglycerol					565:586	diphosphatidylglycerol	565:586	diphosphatidylglycerol	565:586	The strain showed chemotaxonomical properties consistent with its classification in the genus Micromonospora such as meso- and 3-OH-A2pm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids.
31596193	4	46	from	meso-	476:480	arg1	hydrolysate					549:559	whole-cell hydrolysate	538:559	whole-cell hydrolysate	538:559	The strain showed chemotaxonomical properties consistent with its classification in the genus Micromonospora such as meso- and 3-OH-A2pm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids.
31596193	4	46	from	meso-	476:480	arg1	peptidoglycan					513:525	the cell-wall peptidoglycan	499:525	the cell-wall peptidoglycan	499:525	The strain showed chemotaxonomical properties consistent with its classification in the genus Micromonospora such as meso- and 3-OH-A2pm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids.
31596193	6	47	theme	identity	1005:1012	arg1	analyses					1014:1021	Digital DNA-DNA hybridization and average nucleotide identity analyses	952:1021	Digital DNA-DNA hybridization and average nucleotide identity analyses in addition to gyrB gene analysis	952:1055	Digital DNA-DNA hybridization and average nucleotide identity analyses in addition to gyrB gene analysis confirmed the assignment of the strain to a novel species within the genus Micromonospora for which the name Micromonospora deserti sp.
31596193	6	48	theme	average	986:992	arg1	identity					1005:1012	average nucleotide identity	986:1012	average nucleotide identity	986:1012	Digital DNA-DNA hybridization and average nucleotide identity analyses in addition to gyrB gene analysis confirmed the assignment of the strain to a novel species within the genus Micromonospora for which the name Micromonospora deserti sp.
31596193	9	49	theme	type	1292:1295	arg1	strain					1297:1302	the type strain	1288:1302	the type strain	1288:1302	The DNA G+C content of the type strain is 72.4 mol%.
31596193	2	50	theme	novel	201:205	arg1	actinobacteria					207:220	novel actinobacteria	201:220	novel actinobacteria with biosynthetic potential from the Karakum Desert	201:272	An isolate, 13K206T, with typical morphological characteristics of the genus Micromonospora was obtained during a study searching for novel actinobacteria with biosynthetic potential from the Karakum Desert.
31596193	6	51	theme	nucleotide	994:1003	arg1	identity					1005:1012	average nucleotide identity	986:1012	average nucleotide identity	986:1012	Digital DNA-DNA hybridization and average nucleotide identity analyses in addition to gyrB gene analysis confirmed the assignment of the strain to a novel species within the genus Micromonospora for which the name Micromonospora deserti sp.
31596193	2	52	theme	Karakum	259:265	arg1	Desert					267:272	the Karakum Desert	255:272	the Karakum Desert	255:272	An isolate, 13K206T, with typical morphological characteristics of the genus Micromonospora was obtained during a study searching for novel actinobacteria with biosynthetic potential from the Karakum Desert.
31596193	2	53	with	isolate	70:76	arg1	characteristics					115:129	typical morphological characteristics	93:129	typical morphological characteristics of the genus Micromonospora	93:157	An isolate, 13K206T, with typical morphological characteristics of the genus Micromonospora was obtained during a study searching for novel actinobacteria with biosynthetic potential from the Karakum Desert.
31596193	2	54	from	Desert	267:272	arg1	actinobacteria					207:220	novel actinobacteria	201:220	novel actinobacteria with biosynthetic potential from the Karakum Desert	201:272	An isolate, 13K206T, with typical morphological characteristics of the genus Micromonospora was obtained during a study searching for novel actinobacteria with biosynthetic potential from the Karakum Desert.
31596193	6	55	theme	deserti	1181:1187	arg1	sp					1189:1190	the name Micromonospora deserti sp	1157:1190	the name Micromonospora deserti sp	1157:1190	Digital DNA-DNA hybridization and average nucleotide identity analyses in addition to gyrB gene analysis confirmed the assignment of the strain to a novel species within the genus Micromonospora for which the name Micromonospora deserti sp.
31596193	6	56	theme	name	1161:1164	arg1	sp					1189:1190	the name Micromonospora deserti sp	1157:1190	the name Micromonospora deserti sp	1157:1190	Digital DNA-DNA hybridization and average nucleotide identity analyses in addition to gyrB gene analysis confirmed the assignment of the strain to a novel species within the genus Micromonospora for which the name Micromonospora deserti sp.
31596193	6	57	theme	strain	1089:1094	arg1	assignment					1071:1080	the assignment	1067:1080	the assignment of the strain to a novel species within the genus Micromonospora for which the name Micromonospora deserti sp	1067:1190	Digital DNA-DNA hybridization and average nucleotide identity analyses in addition to gyrB gene analysis confirmed the assignment of the strain to a novel species within the genus Micromonospora for which the name Micromonospora deserti sp.
31596193	4	58	theme	genus	447:451	arg1	Micromonospora					453:466	the genus Micromonospora	443:466	the genus Micromonospora	443:466	The strain showed chemotaxonomical properties consistent with its classification in the genus Micromonospora such as meso- and 3-OH-A2pm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids.
31596193	6	59	theme	Micromonospora	1166:1179	arg1	sp					1189:1190	the name Micromonospora deserti sp	1157:1190	the name Micromonospora deserti sp	1157:1190	Digital DNA-DNA hybridization and average nucleotide identity analyses in addition to gyrB gene analysis confirmed the assignment of the strain to a novel species within the genus Micromonospora for which the name Micromonospora deserti sp.
31596193	9	60	theme	strain	1297:1302	arg1	%					1315:1315	72.4 mol%	1307:1315	72.4 mol%	1307:1315	The DNA G+C content of the type strain is 72.4 mol%.
31596193	9	60	theme	strain	1297:1302	arg1	content					1277:1283	The DNA G+C content	1265:1283	The DNA G+C content of the type strain	1265:1302	The DNA G+C content of the type strain is 72.4 mol%.
31596193	8	61	theme	32583T=DSM	1244:1253	arg1	107532T					1255:1261	=JCM 32583T=DSM 107532T	1239:1261	=JCM 32583T=DSM 107532T	1239:1261	The type strain is 13K206T (=JCM 32583T=DSM 107532T).
31596193	8	61	theme	32583T=DSM	1244:1253	arg1	13K206T					1230:1236	13K206T	1230:1236	13K206T (=JCM 32583T=DSM 107532T)	1230:1262	The type strain is 13K206T (=JCM 32583T=DSM 107532T).
31596193	2	62	theme	morphological	101:113	arg1	characteristics					115:129	typical morphological characteristics	93:129	typical morphological characteristics of the genus Micromonospora	93:157	An isolate, 13K206T, with typical morphological characteristics of the genus Micromonospora was obtained during a study searching for novel actinobacteria with biosynthetic potential from the Karakum Desert.
31596193	5	63	theme	sequence	890:897	arg1	similarities					899:910	sequence similarities	890:910	sequence similarities of 98.6, 98.5 and 98.4 %, respectively	890:949	The results of phylogenetic analysis based on 16S rRNA gene sequences revealed that the strain was closely related to 'Micromonospora spongicola' S3-1T, Micromonospora nigra DSM 43818T and Micromonospora yasonensis DS3186T with sequence similarities of 98.6, 98.5 and 98.4 %, respectively.
31596193	4	64	from	phosphatidylinositol	618:637	arg1	diphosphatidylglycerol					565:586	diphosphatidylglycerol	565:586	diphosphatidylglycerol	565:586	The strain showed chemotaxonomical properties consistent with its classification in the genus Micromonospora such as meso- and 3-OH-A2pm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids.
31596193	4	64	from	phosphatidylinositol	618:637	arg1	hydrolysate					549:559	whole-cell hydrolysate	538:559	whole-cell hydrolysate	538:559	The strain showed chemotaxonomical properties consistent with its classification in the genus Micromonospora such as meso- and 3-OH-A2pm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids.
31596193	4	64	from	phosphatidylinositol	618:637	arg1	peptidoglycan					513:525	the cell-wall peptidoglycan	499:525	the cell-wall peptidoglycan	499:525	The strain showed chemotaxonomical properties consistent with its classification in the genus Micromonospora such as meso- and 3-OH-A2pm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids.
33401229	4	0	theme	overall	846:852	arg1	results					854:860	the overall results	842:860	the overall results	842:860	The anticancer activity of the Niclosamide and the Xyl-LA/Nic NPs against the colon carcinoma cell lines (HCT-15, Colo-320) were evaluated by MTT assay and the overall results indicate that the Xyl-LA/Nic NPs significantly enhanced the therapeutic efficiency of niclosamide in cancer therapy.
33401229	4	1	theme	Xyl-LA/Nic	880:889	arg1	NPs					891:893	the Xyl-LA/Nic NPs	876:893	the Xyl-LA/Nic NPs	876:893	The anticancer activity of the Niclosamide and the Xyl-LA/Nic NPs against the colon carcinoma cell lines (HCT-15, Colo-320) were evaluated by MTT assay and the overall results indicate that the Xyl-LA/Nic NPs significantly enhanced the therapeutic efficiency of niclosamide in cancer therapy.
33401229	1	2	theme	novel	149:153	arg1	nanoparticles					171:183	novel redox-sensitive nanoparticles	149:183	novel redox-sensitive nanoparticles based on xylan-lipoic acid (Xyl-LA) conjugate	149:229	In this study, novel redox-sensitive nanoparticles based on xylan-lipoic acid (Xyl-LA) conjugate were developed for tumor targeted delivery of niclosamide (Nic) in cancer therapy.
33401229	1	3	theme	tumor	250:254	arg1	delivery					265:272	tumor targeted delivery	250:272	tumor targeted delivery of niclosamide (Nic) in cancer therapy	250:311	In this study, novel redox-sensitive nanoparticles based on xylan-lipoic acid (Xyl-LA) conjugate were developed for tumor targeted delivery of niclosamide (Nic) in cancer therapy.
33401229	0	4	theme	drug	86:89	arg1	delivery					91:98	tumor targeted drug delivery	71:98	tumor targeted drug delivery of niclosamide in cancer therapy	71:131	Redox-sensitive nanoparticles based on xylan-lipoic acid conjugate for tumor targeted drug delivery of niclosamide in cancer therapy.
33401229	2	5	theme	acid	350:353	arg1	conjugate					355:363	xylan-lipoic acid conjugate	337:363	The niclosamide loaded xylan-lipoic acid conjugate nanoparticles (Xyl-LA/Nic NPs)	314:394	The niclosamide loaded xylan-lipoic acid conjugate nanoparticles (Xyl-LA/Nic NPs) showed redox responsive behaviour in presence of reductive glutathione (GSH), which indicate their suitability for intracellular drug release.
33401229	1	6	theme	targeted	256:263	arg1	delivery					265:272	tumor targeted delivery	250:272	tumor targeted delivery of niclosamide (Nic) in cancer therapy	250:311	In this study, novel redox-sensitive nanoparticles based on xylan-lipoic acid (Xyl-LA) conjugate were developed for tumor targeted delivery of niclosamide (Nic) in cancer therapy.
33401229	0	7	theme	niclosamide	103:113	arg1	delivery					91:98	tumor targeted drug delivery	71:98	tumor targeted drug delivery of niclosamide in cancer therapy	71:131	Redox-sensitive nanoparticles based on xylan-lipoic acid conjugate for tumor targeted drug delivery of niclosamide in cancer therapy.
33401229	3	8	theme	obtained	543:550	arg1	NPs					563:565	The obtained Xyl-LA/Nic NPs	539:565	The obtained Xyl-LA/Nic NPs	539:565	The obtained Xyl-LA/Nic NPs exhibited uniform particle size (196 ± 1.64 nm), high loading capacity (~28.6 wt %) and excellent blood compatibility.
33401229	4	9	theme	NPs	748:750	arg1	activity					701:708	The anticancer activity	686:708	The anticancer activity of the Niclosamide and the Xyl-LA/Nic NPs against the colon carcinoma cell lines (HCT-15, Colo-320)	686:808	The anticancer activity of the Niclosamide and the Xyl-LA/Nic NPs against the colon carcinoma cell lines (HCT-15, Colo-320) were evaluated by MTT assay and the overall results indicate that the Xyl-LA/Nic NPs significantly enhanced the therapeutic efficiency of niclosamide in cancer therapy.
33401229	2	10	theme	drug	525:528	arg1	release					530:536	intracellular drug release	511:536	intracellular drug release	511:536	The niclosamide loaded xylan-lipoic acid conjugate nanoparticles (Xyl-LA/Nic NPs) showed redox responsive behaviour in presence of reductive glutathione (GSH), which indicate their suitability for intracellular drug release.
33401229	3	11	theme	Xyl-LA/Nic	552:561	arg1	NPs					563:565	The obtained Xyl-LA/Nic NPs	539:565	The obtained Xyl-LA/Nic NPs	539:565	The obtained Xyl-LA/Nic NPs exhibited uniform particle size (196 ± 1.64 nm), high loading capacity (~28.6 wt %) and excellent blood compatibility.
33401229	2	12	theme	conjugate	355:363	arg1	nanoparticles					365:377	The niclosamide loaded xylan-lipoic acid conjugate nanoparticles	314:377	The niclosamide loaded xylan-lipoic acid conjugate nanoparticles (Xyl-LA/Nic NPs)	314:394	The niclosamide loaded xylan-lipoic acid conjugate nanoparticles (Xyl-LA/Nic NPs) showed redox responsive behaviour in presence of reductive glutathione (GSH), which indicate their suitability for intracellular drug release.
33401229	2	12	theme	conjugate	355:363	arg1	NPs					391:393	Xyl-LA/Nic NPs	380:393	Xyl-LA/Nic NPs	380:393	The niclosamide loaded xylan-lipoic acid conjugate nanoparticles (Xyl-LA/Nic NPs) showed redox responsive behaviour in presence of reductive glutathione (GSH), which indicate their suitability for intracellular drug release.
33401229	0	13	theme	cancer	118:123	arg1	therapy					125:131	cancer therapy	118:131	cancer therapy	118:131	Redox-sensitive nanoparticles based on xylan-lipoic acid conjugate for tumor targeted drug delivery of niclosamide in cancer therapy.
33401229	4	14	theme	MTT	828:830	arg1	assay					832:836	MTT assay	828:836	MTT assay	828:836	The anticancer activity of the Niclosamide and the Xyl-LA/Nic NPs against the colon carcinoma cell lines (HCT-15, Colo-320) were evaluated by MTT assay and the overall results indicate that the Xyl-LA/Nic NPs significantly enhanced the therapeutic efficiency of niclosamide in cancer therapy.
33401229	4	15	theme	Xyl-LA/Nic	737:746	arg1	NPs					748:750	the Xyl-LA/Nic NPs	733:750	the Xyl-LA/Nic NPs against the colon carcinoma cell lines	733:789	The anticancer activity of the Niclosamide and the Xyl-LA/Nic NPs against the colon carcinoma cell lines (HCT-15, Colo-320) were evaluated by MTT assay and the overall results indicate that the Xyl-LA/Nic NPs significantly enhanced the therapeutic efficiency of niclosamide in cancer therapy.
33401229	4	16	theme	cell	780:783	arg1	lines					785:789	the colon carcinoma cell lines	760:789	the colon carcinoma cell lines	760:789	The anticancer activity of the Niclosamide and the Xyl-LA/Nic NPs against the colon carcinoma cell lines (HCT-15, Colo-320) were evaluated by MTT assay and the overall results indicate that the Xyl-LA/Nic NPs significantly enhanced the therapeutic efficiency of niclosamide in cancer therapy.
33401229	4	17	theme	niclosamide	948:958	arg1	efficiency					934:943	the therapeutic efficiency	918:943	the therapeutic efficiency of niclosamide	918:958	The anticancer activity of the Niclosamide and the Xyl-LA/Nic NPs against the colon carcinoma cell lines (HCT-15, Colo-320) were evaluated by MTT assay and the overall results indicate that the Xyl-LA/Nic NPs significantly enhanced the therapeutic efficiency of niclosamide in cancer therapy.
33401229	2	18	theme	reductive	445:453	arg1	GSH					468:470	GSH	468:470	GSH	468:470	The niclosamide loaded xylan-lipoic acid conjugate nanoparticles (Xyl-LA/Nic NPs) showed redox responsive behaviour in presence of reductive glutathione (GSH), which indicate their suitability for intracellular drug release.
33401229	2	18	theme	reductive	445:453	arg1	glutathione					455:465	reductive glutathione	445:465	reductive glutathione (GSH)	445:471	The niclosamide loaded xylan-lipoic acid conjugate nanoparticles (Xyl-LA/Nic NPs) showed redox responsive behaviour in presence of reductive glutathione (GSH), which indicate their suitability for intracellular drug release.
33401229	2	19	theme	intracellular	511:523	arg1	release					530:536	intracellular drug release	511:536	intracellular drug release	511:536	The niclosamide loaded xylan-lipoic acid conjugate nanoparticles (Xyl-LA/Nic NPs) showed redox responsive behaviour in presence of reductive glutathione (GSH), which indicate their suitability for intracellular drug release.
33401229	2	20	theme	glutathione	455:465	arg1	presence					433:440	presence	433:440	presence of reductive glutathione (GSH), which indicate their suitability for intracellular drug release	433:536	The niclosamide loaded xylan-lipoic acid conjugate nanoparticles (Xyl-LA/Nic NPs) showed redox responsive behaviour in presence of reductive glutathione (GSH), which indicate their suitability for intracellular drug release.
33401229	3	21	theme	uniform	577:583	arg1	196 ± 1.64 nm					600:612	196 ± 1.64 nm	600:612	196 ± 1.64 nm	600:612	The obtained Xyl-LA/Nic NPs exhibited uniform particle size (196 ± 1.64 nm), high loading capacity (~28.6 wt %) and excellent blood compatibility.
33401229	3	21	theme	uniform	577:583	arg1	size					594:597	uniform particle size	577:597	uniform particle size (196 ± 1.64 nm)	577:613	The obtained Xyl-LA/Nic NPs exhibited uniform particle size (196 ± 1.64 nm), high loading capacity (~28.6 wt %) and excellent blood compatibility.
33401229	0	22	theme	Redox-sensitive	0:14	arg1	nanoparticles					16:28	Redox-sensitive nanoparticles	0:28	Redox-sensitive nanoparticles	0:28	Redox-sensitive nanoparticles based on xylan-lipoic acid conjugate for tumor targeted drug delivery of niclosamide in cancer therapy.
33401229	1	23	theme	niclosamide	277:287	arg1	delivery					265:272	tumor targeted delivery	250:272	tumor targeted delivery of niclosamide (Nic) in cancer therapy	250:311	In this study, novel redox-sensitive nanoparticles based on xylan-lipoic acid (Xyl-LA) conjugate were developed for tumor targeted delivery of niclosamide (Nic) in cancer therapy.
33401229	3	24	theme	particle	585:592	arg1	196 ± 1.64 nm					600:612	196 ± 1.64 nm	600:612	196 ± 1.64 nm	600:612	The obtained Xyl-LA/Nic NPs exhibited uniform particle size (196 ± 1.64 nm), high loading capacity (~28.6 wt %) and excellent blood compatibility.
33401229	3	24	theme	particle	585:592	arg1	size					594:597	uniform particle size	577:597	uniform particle size (196 ± 1.64 nm)	577:613	The obtained Xyl-LA/Nic NPs exhibited uniform particle size (196 ± 1.64 nm), high loading capacity (~28.6 wt %) and excellent blood compatibility.
33401229	1	25	theme	xylan-lipoic	194:205	arg1	conjugate					221:229	xylan-lipoic acid (Xyl-LA) conjugate	194:229	xylan-lipoic acid (Xyl-LA) conjugate	194:229	In this study, novel redox-sensitive nanoparticles based on xylan-lipoic acid (Xyl-LA) conjugate were developed for tumor targeted delivery of niclosamide (Nic) in cancer therapy.
33401229	1	26	from	delivery	265:272	arg1	therapy					305:311	cancer therapy	298:311	cancer therapy	298:311	In this study, novel redox-sensitive nanoparticles based on xylan-lipoic acid (Xyl-LA) conjugate were developed for tumor targeted delivery of niclosamide (Nic) in cancer therapy.
33401229	1	27	theme	acid	207:210	arg1	conjugate					221:229	xylan-lipoic acid (Xyl-LA) conjugate	194:229	xylan-lipoic acid (Xyl-LA) conjugate	194:229	In this study, novel redox-sensitive nanoparticles based on xylan-lipoic acid (Xyl-LA) conjugate were developed for tumor targeted delivery of niclosamide (Nic) in cancer therapy.
33401229	4	28	dep	Niclosamide	717:727	arg1	the					713:715	the	713:715	the	713:715	The anticancer activity of the Niclosamide and the Xyl-LA/Nic NPs against the colon carcinoma cell lines (HCT-15, Colo-320) were evaluated by MTT assay and the overall results indicate that the Xyl-LA/Nic NPs significantly enhanced the therapeutic efficiency of niclosamide in cancer therapy.
33401229	4	28	dep	Niclosamide	717:727	arg1	Colo-320					800:807	Colo-320	800:807	Colo-320	800:807	The anticancer activity of the Niclosamide and the Xyl-LA/Nic NPs against the colon carcinoma cell lines (HCT-15, Colo-320) were evaluated by MTT assay and the overall results indicate that the Xyl-LA/Nic NPs significantly enhanced the therapeutic efficiency of niclosamide in cancer therapy.
33401229	0	29	from	delivery	91:98	arg1	therapy					125:131	cancer therapy	118:131	cancer therapy	118:131	Redox-sensitive nanoparticles based on xylan-lipoic acid conjugate for tumor targeted drug delivery of niclosamide in cancer therapy.
33401229	1	30	theme	redox-sensitive	155:169	arg1	nanoparticles					171:183	novel redox-sensitive nanoparticles	149:183	novel redox-sensitive nanoparticles based on xylan-lipoic acid (Xyl-LA) conjugate	149:229	In this study, novel redox-sensitive nanoparticles based on xylan-lipoic acid (Xyl-LA) conjugate were developed for tumor targeted delivery of niclosamide (Nic) in cancer therapy.
33401229	0	31	theme	acid	52:55	arg1	conjugate					57:65	xylan-lipoic acid conjugate	39:65	xylan-lipoic acid conjugate for tumor targeted drug delivery of niclosamide in cancer therapy	39:131	Redox-sensitive nanoparticles based on xylan-lipoic acid conjugate for tumor targeted drug delivery of niclosamide in cancer therapy.
33401229	4	32	theme	therapeutic	922:932	arg1	efficiency					934:943	the therapeutic efficiency	918:943	the therapeutic efficiency of niclosamide	918:958	The anticancer activity of the Niclosamide and the Xyl-LA/Nic NPs against the colon carcinoma cell lines (HCT-15, Colo-320) were evaluated by MTT assay and the overall results indicate that the Xyl-LA/Nic NPs significantly enhanced the therapeutic efficiency of niclosamide in cancer therapy.
33401229	2	33	theme	responsive	409:418	arg1	behaviour					420:428	redox responsive behaviour	403:428	redox responsive behaviour	403:428	The niclosamide loaded xylan-lipoic acid conjugate nanoparticles (Xyl-LA/Nic NPs) showed redox responsive behaviour in presence of reductive glutathione (GSH), which indicate their suitability for intracellular drug release.
33401229	2	34	theme	Xyl-LA/Nic	380:389	arg1	nanoparticles					365:377	The niclosamide loaded xylan-lipoic acid conjugate nanoparticles	314:377	The niclosamide loaded xylan-lipoic acid conjugate nanoparticles (Xyl-LA/Nic NPs)	314:394	The niclosamide loaded xylan-lipoic acid conjugate nanoparticles (Xyl-LA/Nic NPs) showed redox responsive behaviour in presence of reductive glutathione (GSH), which indicate their suitability for intracellular drug release.
33401229	2	34	theme	Xyl-LA/Nic	380:389	arg1	NPs					391:393	Xyl-LA/Nic NPs	380:393	Xyl-LA/Nic NPs	380:393	The niclosamide loaded xylan-lipoic acid conjugate nanoparticles (Xyl-LA/Nic NPs) showed redox responsive behaviour in presence of reductive glutathione (GSH), which indicate their suitability for intracellular drug release.
33401229	1	35	theme	Xyl-LA	213:218	arg1	conjugate					221:229	xylan-lipoic acid (Xyl-LA) conjugate	194:229	xylan-lipoic acid (Xyl-LA) conjugate	194:229	In this study, novel redox-sensitive nanoparticles based on xylan-lipoic acid (Xyl-LA) conjugate were developed for tumor targeted delivery of niclosamide (Nic) in cancer therapy.
33401229	0	36	theme	xylan-lipoic	39:50	arg1	conjugate					57:65	xylan-lipoic acid conjugate	39:65	xylan-lipoic acid conjugate for tumor targeted drug delivery of niclosamide in cancer therapy	39:131	Redox-sensitive nanoparticles based on xylan-lipoic acid conjugate for tumor targeted drug delivery of niclosamide in cancer therapy.
33401229	1	37	theme	cancer	298:303	arg1	therapy					305:311	cancer therapy	298:311	cancer therapy	298:311	In this study, novel redox-sensitive nanoparticles based on xylan-lipoic acid (Xyl-LA) conjugate were developed for tumor targeted delivery of niclosamide (Nic) in cancer therapy.
33401229	4	38	theme	cancer	963:968	arg1	therapy					970:976	cancer therapy	963:976	cancer therapy	963:976	The anticancer activity of the Niclosamide and the Xyl-LA/Nic NPs against the colon carcinoma cell lines (HCT-15, Colo-320) were evaluated by MTT assay and the overall results indicate that the Xyl-LA/Nic NPs significantly enhanced the therapeutic efficiency of niclosamide in cancer therapy.
33401229	2	39	theme	xylan-lipoic	337:348	arg1	conjugate					355:363	xylan-lipoic acid conjugate	337:363	The niclosamide loaded xylan-lipoic acid conjugate nanoparticles (Xyl-LA/Nic NPs)	314:394	The niclosamide loaded xylan-lipoic acid conjugate nanoparticles (Xyl-LA/Nic NPs) showed redox responsive behaviour in presence of reductive glutathione (GSH), which indicate their suitability for intracellular drug release.
33401229	3	40	theme	blood	665:669	arg1	compatibility					671:683	excellent blood compatibility	655:683	excellent blood compatibility	655:683	The obtained Xyl-LA/Nic NPs exhibited uniform particle size (196 ± 1.64 nm), high loading capacity (~28.6 wt %) and excellent blood compatibility.
33401229	2	41	theme	loaded	330:335	arg1	nanoparticles					365:377	The niclosamide loaded xylan-lipoic acid conjugate nanoparticles	314:377	The niclosamide loaded xylan-lipoic acid conjugate nanoparticles (Xyl-LA/Nic NPs)	314:394	The niclosamide loaded xylan-lipoic acid conjugate nanoparticles (Xyl-LA/Nic NPs) showed redox responsive behaviour in presence of reductive glutathione (GSH), which indicate their suitability for intracellular drug release.
33401229	2	41	theme	loaded	330:335	arg1	NPs					391:393	Xyl-LA/Nic NPs	380:393	Xyl-LA/Nic NPs	380:393	The niclosamide loaded xylan-lipoic acid conjugate nanoparticles (Xyl-LA/Nic NPs) showed redox responsive behaviour in presence of reductive glutathione (GSH), which indicate their suitability for intracellular drug release.
33401229	3	42	theme	loading	621:627	arg1	%					648:648	~28.6 wt %	639:648	~28.6 wt %	639:648	The obtained Xyl-LA/Nic NPs exhibited uniform particle size (196 ± 1.64 nm), high loading capacity (~28.6 wt %) and excellent blood compatibility.
33401229	3	42	theme	loading	621:627	arg1	capacity					629:636	high loading capacity	616:636	high loading capacity (~28.6 wt %)	616:649	The obtained Xyl-LA/Nic NPs exhibited uniform particle size (196 ± 1.64 nm), high loading capacity (~28.6 wt %) and excellent blood compatibility.
33401229	2	43	theme	niclosamide	318:328	arg1	nanoparticles					365:377	The niclosamide loaded xylan-lipoic acid conjugate nanoparticles	314:377	The niclosamide loaded xylan-lipoic acid conjugate nanoparticles (Xyl-LA/Nic NPs)	314:394	The niclosamide loaded xylan-lipoic acid conjugate nanoparticles (Xyl-LA/Nic NPs) showed redox responsive behaviour in presence of reductive glutathione (GSH), which indicate their suitability for intracellular drug release.
33401229	2	43	theme	niclosamide	318:328	arg1	NPs					391:393	Xyl-LA/Nic NPs	380:393	Xyl-LA/Nic NPs	380:393	The niclosamide loaded xylan-lipoic acid conjugate nanoparticles (Xyl-LA/Nic NPs) showed redox responsive behaviour in presence of reductive glutathione (GSH), which indicate their suitability for intracellular drug release.
33401229	4	44	theme	anticancer	690:699	arg1	activity					701:708	The anticancer activity	686:708	The anticancer activity of the Niclosamide and the Xyl-LA/Nic NPs against the colon carcinoma cell lines (HCT-15, Colo-320)	686:808	The anticancer activity of the Niclosamide and the Xyl-LA/Nic NPs against the colon carcinoma cell lines (HCT-15, Colo-320) were evaluated by MTT assay and the overall results indicate that the Xyl-LA/Nic NPs significantly enhanced the therapeutic efficiency of niclosamide in cancer therapy.
33401229	4	45	theme	carcinoma	770:778	arg1	lines					785:789	the colon carcinoma cell lines	760:789	the colon carcinoma cell lines	760:789	The anticancer activity of the Niclosamide and the Xyl-LA/Nic NPs against the colon carcinoma cell lines (HCT-15, Colo-320) were evaluated by MTT assay and the overall results indicate that the Xyl-LA/Nic NPs significantly enhanced the therapeutic efficiency of niclosamide in cancer therapy.
33401229	4	46	theme	colon	764:768	arg1	lines					785:789	the colon carcinoma cell lines	760:789	the colon carcinoma cell lines	760:789	The anticancer activity of the Niclosamide and the Xyl-LA/Nic NPs against the colon carcinoma cell lines (HCT-15, Colo-320) were evaluated by MTT assay and the overall results indicate that the Xyl-LA/Nic NPs significantly enhanced the therapeutic efficiency of niclosamide in cancer therapy.
33401229	4	47	theme	Niclosamide	717:727	arg1	activity					701:708	The anticancer activity	686:708	The anticancer activity of the Niclosamide and the Xyl-LA/Nic NPs against the colon carcinoma cell lines (HCT-15, Colo-320)	686:808	The anticancer activity of the Niclosamide and the Xyl-LA/Nic NPs against the colon carcinoma cell lines (HCT-15, Colo-320) were evaluated by MTT assay and the overall results indicate that the Xyl-LA/Nic NPs significantly enhanced the therapeutic efficiency of niclosamide in cancer therapy.
33401229	0	48	theme	targeted	77:84	arg1	delivery					91:98	tumor targeted drug delivery	71:98	tumor targeted drug delivery of niclosamide in cancer therapy	71:131	Redox-sensitive nanoparticles based on xylan-lipoic acid conjugate for tumor targeted drug delivery of niclosamide in cancer therapy.
33401229	3	49	theme	excellent	655:663	arg1	compatibility					671:683	excellent blood compatibility	655:683	excellent blood compatibility	655:683	The obtained Xyl-LA/Nic NPs exhibited uniform particle size (196 ± 1.64 nm), high loading capacity (~28.6 wt %) and excellent blood compatibility.
33401229	0	50	theme	tumor	71:75	arg1	delivery					91:98	tumor targeted drug delivery	71:98	tumor targeted drug delivery of niclosamide in cancer therapy	71:131	Redox-sensitive nanoparticles based on xylan-lipoic acid conjugate for tumor targeted drug delivery of niclosamide in cancer therapy.
33401229	3	51	theme	high	616:619	arg1	%					648:648	~28.6 wt %	639:648	~28.6 wt %	639:648	The obtained Xyl-LA/Nic NPs exhibited uniform particle size (196 ± 1.64 nm), high loading capacity (~28.6 wt %) and excellent blood compatibility.
33401229	3	51	theme	high	616:619	arg1	capacity					629:636	high loading capacity	616:636	high loading capacity (~28.6 wt %)	616:649	The obtained Xyl-LA/Nic NPs exhibited uniform particle size (196 ± 1.64 nm), high loading capacity (~28.6 wt %) and excellent blood compatibility.
34562563	14	0	theme	cells	2430:2434	arg1	treatment					2396:2404	GSO treatment	2392:2404	GSO treatment of UVB-irradiated HaCaT cells	2392:2434	In vitro, GSO treatment of UVB-irradiated HaCaT cells led to increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins, while mRNA levels of desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1 and associated protein levels were restored to normal levels.
34562563	6	1	theme	epidermal	1118:1126	arg1	thickness					1128:1136	epidermal thickness	1118:1136	epidermal thickness	1118:1136	To evaluate GSO as a skin barrier repair-promoting treatment, skin of UVB-irradiated BALB/c hairless mice was treated with or without GSO then skin samples were evaluated for epidermal thickness, transepidermal water loss (TEWL), and stratum corneum water content.
34562563	15	2	theme	skin	2730:2733	arg1	damage					2743:2748	UVB-induced skin barrier damage	2718:2748	UVB-induced skin barrier damage	2718:2748	CONCLUSION A P. ginseng oligosaccharide preparation repaired UVB-induced skin barrier damage by alleviating skin dryness and desquamation symptoms, highlighting its potential as a natural cosmetic additive that can promote skin barrier repair after UVB exposure.
34562563	15	3	theme	UVB-induced	2718:2728	arg1	damage					2743:2748	UVB-induced skin barrier damage	2718:2748	UVB-induced skin barrier damage	2718:2748	CONCLUSION A P. ginseng oligosaccharide preparation repaired UVB-induced skin barrier damage by alleviating skin dryness and desquamation symptoms, highlighting its potential as a natural cosmetic additive that can promote skin barrier repair after UVB exposure.
34562563	14	4	theme	UVB-irradiated	2409:2422	arg1	cells					2430:2434	UVB-irradiated HaCaT cells	2409:2434	UVB-irradiated HaCaT cells	2409:2434	In vitro, GSO treatment of UVB-irradiated HaCaT cells led to increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins, while mRNA levels of desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1 and associated protein levels were restored to normal levels.
34562563	7	5	theme	HaCaT	1250:1254	arg1	cells					1256:1260	HaCaT cells	1250:1260	HaCaT cells	1250:1260	In addition, UVB-exposed skin samples and HaCaT cells were analyzed to assess GSO treatment effects on levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3).
34562563	8	6	theme	trypsin-like	1641:1652	arg1	peptidase					1673:1681	trypsin-like kallikrein-related peptidase 5	1641:1683	trypsin-like kallikrein-related peptidase 5 (KLK5)	1641:1690	Meanwhile, GSO treatment was also evaluated for effects on UVB-irradiated hairless mouse skin and HaCaT cells based on levels of serine protease inhibitor Kazal type-5 (SPINK5), trypsin-like kallikrein-related peptidase 5 (KLK5), chymotrypsin-like KLK7, and desmoglein 1 (DSG1).
34562563	8	6	theme	trypsin-like	1641:1652	arg1	KLK5					1686:1689	KLK5	1686:1689	KLK5	1686:1689	Meanwhile, GSO treatment was also evaluated for effects on UVB-irradiated hairless mouse skin and HaCaT cells based on levels of serine protease inhibitor Kazal type-5 (SPINK5), trypsin-like kallikrein-related peptidase 5 (KLK5), chymotrypsin-like KLK7, and desmoglein 1 (DSG1).
34562563	11	7	theme	dorsal	2087:2092	arg1	skin					2094:2097	dorsal skin	2087:2097	dorsal skin of BALB/c hairless mice	2087:2121	In vivo, GSO applied to dorsal skin of BALB/c hairless mice attenuated UVB-induced epidermal thickening and moisture loss.
34562563	14	8	theme	normal	2642:2647	arg1	levels					2649:2654	normal levels	2642:2654	normal levels	2642:2654	In vitro, GSO treatment of UVB-irradiated HaCaT cells led to increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins, while mRNA levels of desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1 and associated protein levels were restored to normal levels.
34562563	6	9	theme	corneum	1185:1191	arg1	content					1199:1205	stratum corneum water content	1177:1205	stratum corneum water content	1177:1205	To evaluate GSO as a skin barrier repair-promoting treatment, skin of UVB-irradiated BALB/c hairless mice was treated with or without GSO then skin samples were evaluated for epidermal thickness, transepidermal water loss (TEWL), and stratum corneum water content.
34562563	4	10	theme	oligosaccharide	699:713	arg1	GSO					724:726	GSO	724:726	GSO	724:726	MATERIALS AND METHODS Ginseng oligosaccharide extract (GSO) was prepared from P. ginseng via water extraction followed by ethanol precipitation and resin and gel purification.
34562563	4	10	theme	oligosaccharide	699:713	arg1	extract					715:721	MATERIALS AND METHODS Ginseng oligosaccharide extract	669:721	MATERIALS AND METHODS Ginseng oligosaccharide extract (GSO)	669:727	MATERIALS AND METHODS Ginseng oligosaccharide extract (GSO) was prepared from P. ginseng via water extraction followed by ethanol precipitation and resin and gel purification.
34562563	15	11	theme	oligosaccharide	2681:2695	arg1	preparation					2697:2707	A P. ginseng oligosaccharide preparation	2668:2707	A P. ginseng oligosaccharide preparation	2668:2707	CONCLUSION A P. ginseng oligosaccharide preparation repaired UVB-induced skin barrier damage by alleviating skin dryness and desquamation symptoms, highlighting its potential as a natural cosmetic additive that can promote skin barrier repair after UVB exposure.
34562563	11	12	theme	mice	2118:2121	arg1	skin					2094:2097	dorsal skin	2087:2097	dorsal skin of BALB/c hairless mice	2087:2121	In vivo, GSO applied to dorsal skin of BALB/c hairless mice attenuated UVB-induced epidermal thickening and moisture loss.
34562563	8	13	theme	peptidase	1673:1681	arg1	levels					1582:1587	levels	1582:1587	levels of serine protease inhibitor Kazal type-5 (SPINK5), trypsin-like kallikrein-related peptidase 5 (KLK5), chymotrypsin-like KLK7, and desmoglein 1 (DSG1)	1582:1739	Meanwhile, GSO treatment was also evaluated for effects on UVB-irradiated hairless mouse skin and HaCaT cells based on levels of serine protease inhibitor Kazal type-5 (SPINK5), trypsin-like kallikrein-related peptidase 5 (KLK5), chymotrypsin-like KLK7, and desmoglein 1 (DSG1).
34562563	1	14	theme	RELEVANCE	188:196	arg1	dysfunction					211:221	ETHNOPHARMACOLOGICAL RELEVANCE Skin barrier dysfunction	167:221	ETHNOPHARMACOLOGICAL RELEVANCE Skin barrier dysfunction	167:221	ETHNOPHARMACOLOGICAL RELEVANCE Skin barrier dysfunction can lead to water and electrolyte loss, triggering homeostatic imbalances that can trigger atopic dermatitis and anaphylaxis.
34562563	15	15	theme	P.	2670:2671	arg1	preparation					2697:2707	A P. ginseng oligosaccharide preparation	2668:2707	A P. ginseng oligosaccharide preparation	2668:2707	CONCLUSION A P. ginseng oligosaccharide preparation repaired UVB-induced skin barrier damage by alleviating skin dryness and desquamation symptoms, highlighting its potential as a natural cosmetic additive that can promote skin barrier repair after UVB exposure.
34562563	3	16	theme	ginseng	608:614	arg1	components					623:632	ginseng active components	608:632	ginseng active components	608:632	However, few reports exist that describe effectiveness of ginseng active components for repair of skin barrier damage.
34562563	1	17	theme	barrier	203:209	arg1	dysfunction					211:221	ETHNOPHARMACOLOGICAL RELEVANCE Skin barrier dysfunction	167:221	ETHNOPHARMACOLOGICAL RELEVANCE Skin barrier dysfunction	167:221	ETHNOPHARMACOLOGICAL RELEVANCE Skin barrier dysfunction can lead to water and electrolyte loss, triggering homeostatic imbalances that can trigger atopic dermatitis and anaphylaxis.
34562563	15	18	theme	skin	2880:2883	arg1	repair					2893:2898	skin barrier repair	2880:2898	skin barrier repair	2880:2898	CONCLUSION A P. ginseng oligosaccharide preparation repaired UVB-induced skin barrier damage by alleviating skin dryness and desquamation symptoms, highlighting its potential as a natural cosmetic additive that can promote skin barrier repair after UVB exposure.
34562563	4	19	theme	MATERIALS	669:677	arg1	GSO					724:726	GSO	724:726	GSO	724:726	MATERIALS AND METHODS Ginseng oligosaccharide extract (GSO) was prepared from P. ginseng via water extraction followed by ethanol precipitation and resin and gel purification.
34562563	4	19	theme	MATERIALS	669:677	arg1	extract					715:721	MATERIALS AND METHODS Ginseng oligosaccharide extract	669:721	MATERIALS AND METHODS Ginseng oligosaccharide extract (GSO)	669:727	MATERIALS AND METHODS Ginseng oligosaccharide extract (GSO) was prepared from P. ginseng via water extraction followed by ethanol precipitation and resin and gel purification.
34562563	15	20	theme	desquamation	2782:2793	arg1	symptoms					2795:2802	skin dryness and desquamation symptoms	2765:2802	symptoms	2795:2802	CONCLUSION A P. ginseng oligosaccharide preparation repaired UVB-induced skin barrier damage by alleviating skin dryness and desquamation symptoms, highlighting its potential as a natural cosmetic additive that can promote skin barrier repair after UVB exposure.
34562563	14	21	theme	proteins	2557:2564	arg1	levels					2618:2623	associated protein levels	2599:2623	associated protein levels	2599:2623	In vitro, GSO treatment of UVB-irradiated HaCaT cells led to increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins, while mRNA levels of desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1 and associated protein levels were restored to normal levels.
34562563	14	21	theme	proteins	2557:2564	arg1	levels					2526:2531	mRNA levels	2521:2531	mRNA levels of desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1	2521:2593	In vitro, GSO treatment of UVB-irradiated HaCaT cells led to increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins, while mRNA levels of desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1 and associated protein levels were restored to normal levels.
34562563	7	22	theme	barrier	1378:1384	arg1	involucrin					1421:1430	involucrin	1421:1430	involucrin (IVL)	1421:1436	In addition, UVB-exposed skin samples and HaCaT cells were analyzed to assess GSO treatment effects on levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3).
34562563	7	22	theme	barrier	1378:1384	arg1	filaggrin					1404:1412	filaggrin	1404:1412	filaggrin (FLG)	1404:1418	In addition, UVB-exposed skin samples and HaCaT cells were analyzed to assess GSO treatment effects on levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3).
34562563	7	22	theme	barrier	1378:1384	arg1	aquaporin-3					1443:1453	aquaporin-3	1443:1453	aquaporin-3 (AQP3)	1443:1460	In addition, UVB-exposed skin samples and HaCaT cells were analyzed to assess GSO treatment effects on levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3).
34562563	7	22	theme	barrier	1378:1384	arg1	proteins					1386:1393	other skin barrier proteins	1367:1393	other skin barrier proteins	1367:1393	In addition, UVB-exposed skin samples and HaCaT cells were analyzed to assess GSO treatment effects on levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3).
34562563	6	23	theme	skin	1086:1089	arg1	samples					1091:1097	skin samples	1086:1097	skin samples	1086:1097	To evaluate GSO as a skin barrier repair-promoting treatment, skin of UVB-irradiated BALB/c hairless mice was treated with or without GSO then skin samples were evaluated for epidermal thickness, transepidermal water loss (TEWL), and stratum corneum water content.
34562563	4	24	theme	METHODS	683:689	arg1	GSO					724:726	GSO	724:726	GSO	724:726	MATERIALS AND METHODS Ginseng oligosaccharide extract (GSO) was prepared from P. ginseng via water extraction followed by ethanol precipitation and resin and gel purification.
34562563	4	24	theme	METHODS	683:689	arg1	extract					715:721	MATERIALS AND METHODS Ginseng oligosaccharide extract	669:721	MATERIALS AND METHODS Ginseng oligosaccharide extract (GSO)	669:727	MATERIALS AND METHODS Ginseng oligosaccharide extract (GSO) was prepared from P. ginseng via water extraction followed by ethanol precipitation and resin and gel purification.
34562563	0	25	theme	hairless	128:135	arg1	mice					137:140	BALB/c hairless mice	121:140	BALB/c hairless mice	121:140	Protective effect of oligosaccharides isolated from Panax ginseng C. A. Meyer against UVB-induced skin barrier damage in BALB/c hairless mice and human keratinocytes.
34562563	8	26	theme	chymotrypsin-like	1693:1709	arg1	KLK7					1711:1714	chymotrypsin-like KLK7	1693:1714	chymotrypsin-like KLK7	1693:1714	Meanwhile, GSO treatment was also evaluated for effects on UVB-irradiated hairless mouse skin and HaCaT cells based on levels of serine protease inhibitor Kazal type-5 (SPINK5), trypsin-like kallikrein-related peptidase 5 (KLK5), chymotrypsin-like KLK7, and desmoglein 1 (DSG1).
34562563	6	27	theme	skin	964:967	arg1	barrier					969:975	a skin barrier	962:975	a skin barrier repair-promoting treatment	962:1002	To evaluate GSO as a skin barrier repair-promoting treatment, skin of UVB-irradiated BALB/c hairless mice was treated with or without GSO then skin samples were evaluated for epidermal thickness, transepidermal water loss (TEWL), and stratum corneum water content.
34562563	6	27	theme	skin	964:967	arg1	GSO					955:957	GSO	955:957	GSO	955:957	To evaluate GSO as a skin barrier repair-promoting treatment, skin of UVB-irradiated BALB/c hairless mice was treated with or without GSO then skin samples were evaluated for epidermal thickness, transepidermal water loss (TEWL), and stratum corneum water content.
34562563	7	28	theme	other	1367:1371	arg1	involucrin					1421:1430	involucrin	1421:1430	involucrin (IVL)	1421:1436	In addition, UVB-exposed skin samples and HaCaT cells were analyzed to assess GSO treatment effects on levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3).
34562563	7	28	theme	other	1367:1371	arg1	filaggrin					1404:1412	filaggrin	1404:1412	filaggrin (FLG)	1404:1418	In addition, UVB-exposed skin samples and HaCaT cells were analyzed to assess GSO treatment effects on levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3).
34562563	7	28	theme	other	1367:1371	arg1	aquaporin-3					1443:1453	aquaporin-3	1443:1453	aquaporin-3 (AQP3)	1443:1460	In addition, UVB-exposed skin samples and HaCaT cells were analyzed to assess GSO treatment effects on levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3).
34562563	7	28	theme	other	1367:1371	arg1	proteins					1386:1393	other skin barrier proteins	1367:1393	other skin barrier proteins	1367:1393	In addition, UVB-exposed skin samples and HaCaT cells were analyzed to assess GSO treatment effects on levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3).
34562563	4	29	theme	P.	747:748	arg1	ginseng					750:756	P. ginseng	747:756	P. ginseng	747:756	MATERIALS AND METHODS Ginseng oligosaccharide extract (GSO) was prepared from P. ginseng via water extraction followed by ethanol precipitation and resin and gel purification.
34562563	10	30	theme	distinct	1922:1929	arg1	types					1931:1935	seven distinct types	1916:1935	seven distinct types of monosaccharides with molecular weights of approximately 1 kDa that were covalently linked together via β-glycosidic bonds	1916:2060	RESULTS GSO was shown to consist of oligosaccharides comprised of seven distinct types of monosaccharides with molecular weights of approximately 1 kDa that were covalently linked together via β-glycosidic bonds.
34562563	10	31	attach	linked	2023:2028	arg3	bonds					2056:2060	β-glycosidic bonds	2043:2060	β-glycosidic bonds	2043:2060	RESULTS GSO was shown to consist of oligosaccharides comprised of seven distinct types of monosaccharides with molecular weights of approximately 1 kDa that were covalently linked together via β-glycosidic bonds.
34562563	3	32	theme	components	623:632	arg1	effectiveness					591:603	effectiveness	591:603	effectiveness of ginseng active components for repair of skin barrier damage	591:666	However, few reports exist that describe effectiveness of ginseng active components for repair of skin barrier damage.
34562563	14	33	theme	mRNA	2521:2524	arg1	levels					2526:2531	mRNA levels	2521:2531	mRNA levels of desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1	2521:2593	In vitro, GSO treatment of UVB-irradiated HaCaT cells led to increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins, while mRNA levels of desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1 and associated protein levels were restored to normal levels.
34562563	7	34	theme	protein	1355:1361	arg1	levels					1311:1316	levels	1311:1316	levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3)	1311:1460	In addition, UVB-exposed skin samples and HaCaT cells were analyzed to assess GSO treatment effects on levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3).
34562563	3	35	theme	damage	661:666	arg1	repair					638:643	repair	638:643	repair of skin barrier damage	638:666	However, few reports exist that describe effectiveness of ginseng active components for repair of skin barrier damage.
34562563	8	36	theme	desmoglein	1721:1730	arg1	levels					1582:1587	levels	1582:1587	levels of serine protease inhibitor Kazal type-5 (SPINK5), trypsin-like kallikrein-related peptidase 5 (KLK5), chymotrypsin-like KLK7, and desmoglein 1 (DSG1)	1582:1739	Meanwhile, GSO treatment was also evaluated for effects on UVB-irradiated hairless mouse skin and HaCaT cells based on levels of serine protease inhibitor Kazal type-5 (SPINK5), trypsin-like kallikrein-related peptidase 5 (KLK5), chymotrypsin-like KLK7, and desmoglein 1 (DSG1).
34562563	12	37	theme	AQP3	2265:2268	arg1	proteins					2270:2277	AQP3 proteins	2265:2277	AQP3 proteins	2265:2277	Furthermore, GSO ameliorated UVB-induced reductions of levels of FLG, IVL, and AQP3 proteins.
34562563	8	38	dep	inhibitor	1608:1616	arg1	SPINK5					1632:1637	SPINK5	1632:1637	SPINK5	1632:1637	Meanwhile, GSO treatment was also evaluated for effects on UVB-irradiated hairless mouse skin and HaCaT cells based on levels of serine protease inhibitor Kazal type-5 (SPINK5), trypsin-like kallikrein-related peptidase 5 (KLK5), chymotrypsin-like KLK7, and desmoglein 1 (DSG1).
34562563	8	38	dep	inhibitor	1608:1616	arg1	type-5					1624:1629	Kazal type-5	1618:1629	serine protease inhibitor Kazal type-5 (SPINK5)	1592:1638	Meanwhile, GSO treatment was also evaluated for effects on UVB-irradiated hairless mouse skin and HaCaT cells based on levels of serine protease inhibitor Kazal type-5 (SPINK5), trypsin-like kallikrein-related peptidase 5 (KLK5), chymotrypsin-like KLK7, and desmoglein 1 (DSG1).
34562563	3	39	theme	skin	648:651	arg1	damage					661:666	skin barrier damage	648:666	skin barrier damage	648:666	However, few reports exist that describe effectiveness of ginseng active components for repair of skin barrier damage.
34562563	6	40	theme	mice	1044:1047	arg1	skin					1005:1008	skin	1005:1008	skin of UVB-irradiated BALB/c hairless mice	1005:1047	To evaluate GSO as a skin barrier repair-promoting treatment, skin of UVB-irradiated BALB/c hairless mice was treated with or without GSO then skin samples were evaluated for epidermal thickness, transepidermal water loss (TEWL), and stratum corneum water content.
34562563	7	41	theme	skin	1233:1236	arg1	samples					1238:1244	UVB-exposed skin samples	1221:1244	UVB-exposed skin samples	1221:1244	In addition, UVB-exposed skin samples and HaCaT cells were analyzed to assess GSO treatment effects on levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3).
34562563	6	42	theme	BALB/c	1028:1033	arg1	mice					1044:1047	UVB-irradiated BALB/c hairless mice	1013:1047	UVB-irradiated BALB/c hairless mice	1013:1047	To evaluate GSO as a skin barrier repair-promoting treatment, skin of UVB-irradiated BALB/c hairless mice was treated with or without GSO then skin samples were evaluated for epidermal thickness, transepidermal water loss (TEWL), and stratum corneum water content.
34562563	0	43	theme	UVB-induced	86:96	arg1	damage					111:116	UVB-induced skin barrier damage	86:116	UVB-induced skin barrier damage in BALB/c hairless mice and human keratinocytes	86:164	Protective effect of oligosaccharides isolated from Panax ginseng C. A. Meyer against UVB-induced skin barrier damage in BALB/c hairless mice and human keratinocytes.
34562563	10	44	with	monosaccharides	1940:1954	arg1	weights					1971:1977	molecular weights	1961:1977	molecular weights of approximately 1 kDa that were covalently linked together via β-glycosidic bonds	1961:2060	RESULTS GSO was shown to consist of oligosaccharides comprised of seven distinct types of monosaccharides with molecular weights of approximately 1 kDa that were covalently linked together via β-glycosidic bonds.
34562563	14	45	theme	AQP3	2470:2473	arg1	levels					2480:2485	AQP3 mRNA levels	2470:2485	AQP3 mRNA levels	2470:2485	In vitro, GSO treatment of UVB-irradiated HaCaT cells led to increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins, while mRNA levels of desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1 and associated protein levels were restored to normal levels.
34562563	12	46	theme	UVB-induced	2215:2225	arg1	reductions					2227:2236	UVB-induced reductions	2215:2236	UVB-induced reductions of levels of FLG, IVL, and AQP3 proteins	2215:2277	Furthermore, GSO ameliorated UVB-induced reductions of levels of FLG, IVL, and AQP3 proteins.
34562563	2	47	theme	known	419:423	arg1	benefits					437:444	known therapeutic benefits	419:444	known therapeutic benefits for the treatment of skin diseases, including photodamage repair effects and reduction of pigmentation	419:547	Panax ginseng C.A. Meyer is a traditional Chinese medicinal herb with known therapeutic benefits for the treatment of skin diseases, including photodamage repair effects and reduction of pigmentation.
34562563	0	48	theme	barrier	103:109	arg1	damage					111:116	UVB-induced skin barrier damage	86:116	UVB-induced skin barrier damage in BALB/c hairless mice and human keratinocytes	86:164	Protective effect of oligosaccharides isolated from Panax ginseng C. A. Meyer against UVB-induced skin barrier damage in BALB/c hairless mice and human keratinocytes.
34562563	11	49	theme	epidermal	2146:2154	arg1	thickening					2156:2165	UVB-induced epidermal thickening	2134:2165	UVB-induced epidermal thickening	2134:2165	In vivo, GSO applied to dorsal skin of BALB/c hairless mice attenuated UVB-induced epidermal thickening and moisture loss.
34562563	2	50	theme	diseases	472:479	arg1	treatment					454:462	the treatment	450:462	the treatment of skin diseases, including photodamage repair effects and reduction of pigmentation	450:547	Panax ginseng C.A. Meyer is a traditional Chinese medicinal herb with known therapeutic benefits for the treatment of skin diseases, including photodamage repair effects and reduction of pigmentation.
34562563	0	51	theme	Protective	0:9	arg1	effect					11:16	Protective effect	0:16	Protective effect of oligosaccharides isolated from Panax	0:56	Protective effect of oligosaccharides isolated from Panax ginseng C. A. Meyer against UVB-induced skin barrier damage in BALB/c hairless mice and human keratinocytes.
34562563	13	52	theme	decreased	2352:2360	arg1	expression					2362:2371	decreased expression	2352:2371	decreased expression of KLK7	2352:2379	Additionally, GSO treatment led to increased DSG1 protein levels due to decreased expression of KLK7.
34562563	7	53	theme	cornified	1331:1339	arg1	CE					1351:1352	CE	1351:1352	CE	1351:1352	In addition, UVB-exposed skin samples and HaCaT cells were analyzed to assess GSO treatment effects on levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3).
34562563	7	53	theme	cornified	1331:1339	arg1	envelope					1341:1348	epidermal cornified envelope	1321:1348	epidermal cornified envelope (CE) protein	1321:1361	In addition, UVB-exposed skin samples and HaCaT cells were analyzed to assess GSO treatment effects on levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3).
34562563	5	54	theme	structural	865:874	arg1	characteristics					876:890	structural characteristics	865:890	structural characteristics	865:890	GSO composition and structural characteristics were determined using LC-MS, HPLC, FT-IR, and NMR.
34562563	2	55	theme	repair	504:509	arg1	effects					511:517	photodamage repair effects	492:517	photodamage repair effects	492:517	Panax ginseng C.A. Meyer is a traditional Chinese medicinal herb with known therapeutic benefits for the treatment of skin diseases, including photodamage repair effects and reduction of pigmentation.
34562563	14	56	theme	IVL	2461:2463	arg1	increases					2443:2451	increases	2443:2451	increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins	2443:2512	In vitro, GSO treatment of UVB-irradiated HaCaT cells led to increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins, while mRNA levels of desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1 and associated protein levels were restored to normal levels.
34562563	13	57	theme	protein	2330:2336	arg1	levels					2338:2343	increased DSG1 protein levels	2315:2343	increased DSG1 protein levels due to decreased expression of KLK7	2315:2379	Additionally, GSO treatment led to increased DSG1 protein levels due to decreased expression of KLK7.
34562563	7	58	from	effects	1300:1306	arg1	levels					1311:1316	levels	1311:1316	levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3)	1311:1460	In addition, UVB-exposed skin samples and HaCaT cells were analyzed to assess GSO treatment effects on levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3).
34562563	15	59	theme	UVB	2906:2908	arg1	exposure					2910:2917	UVB exposure	2906:2917	UVB exposure	2906:2917	CONCLUSION A P. ginseng oligosaccharide preparation repaired UVB-induced skin barrier damage by alleviating skin dryness and desquamation symptoms, highlighting its potential as a natural cosmetic additive that can promote skin barrier repair after UVB exposure.
34562563	14	60	dep	proteins	2557:2564	arg1	SPINK5					2566:2571	SPINK5	2566:2571	SPINK5	2566:2571	In vitro, GSO treatment of UVB-irradiated HaCaT cells led to increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins, while mRNA levels of desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1 and associated protein levels were restored to normal levels.
34562563	14	60	dep	proteins	2557:2564	arg1	proteins					2557:2564	desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1	2536:2593	desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1	2536:2593	In vitro, GSO treatment of UVB-irradiated HaCaT cells led to increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins, while mRNA levels of desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1 and associated protein levels were restored to normal levels.
34562563	14	60	dep	proteins	2557:2564	arg1	KLK7					2580:2583	KLK7	2580:2583	KLK7	2580:2583	In vitro, GSO treatment of UVB-irradiated HaCaT cells led to increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins, while mRNA levels of desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1 and associated protein levels were restored to normal levels.
34562563	14	60	dep	proteins	2557:2564	arg1	DSG1					2590:2593	DSG1	2590:2593	DSG1	2590:2593	In vitro, GSO treatment of UVB-irradiated HaCaT cells led to increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins, while mRNA levels of desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1 and associated protein levels were restored to normal levels.
34562563	14	60	dep	proteins	2557:2564	arg1	KLK5					2574:2577	KLK5	2574:2577	KLK5	2574:2577	In vitro, GSO treatment of UVB-irradiated HaCaT cells led to increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins, while mRNA levels of desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1 and associated protein levels were restored to normal levels.
34562563	14	61	theme	FLG	2456:2458	arg1	increases					2443:2451	increases	2443:2451	increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins	2443:2512	In vitro, GSO treatment of UVB-irradiated HaCaT cells led to increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins, while mRNA levels of desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1 and associated protein levels were restored to normal levels.
34562563	13	62	theme	due	2345:2347	arg1	levels					2338:2343	increased DSG1 protein levels	2315:2343	increased DSG1 protein levels due to decreased expression of KLK7	2315:2379	Additionally, GSO treatment led to increased DSG1 protein levels due to decreased expression of KLK7.
34562563	7	63	theme	treatment	1290:1298	arg1	effects					1300:1306	GSO treatment effects	1286:1306	GSO treatment effects on levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3)	1286:1460	In addition, UVB-exposed skin samples and HaCaT cells were analyzed to assess GSO treatment effects on levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3).
34562563	9	64	theme	UVB-induced	1777:1787	arg1	damage					1802:1807	UVB-induced skin barrier damage	1777:1807	UVB-induced skin barrier damage manifesting as dryness and desquamation	1777:1847	These proteins are associated with UVB-induced skin barrier damage manifesting as dryness and desquamation.
34562563	8	65	theme	hairless	1537:1544	arg1	skin					1552:1555	UVB-irradiated hairless mouse skin and HaCaT cells	1522:1571	skin	1552:1555	Meanwhile, GSO treatment was also evaluated for effects on UVB-irradiated hairless mouse skin and HaCaT cells based on levels of serine protease inhibitor Kazal type-5 (SPINK5), trypsin-like kallikrein-related peptidase 5 (KLK5), chymotrypsin-like KLK7, and desmoglein 1 (DSG1).
34562563	4	66	theme	gel	827:829	arg1	purification					831:842	gel purification	827:842	gel purification	827:842	MATERIALS AND METHODS Ginseng oligosaccharide extract (GSO) was prepared from P. ginseng via water extraction followed by ethanol precipitation and resin and gel purification.
34562563	8	67	theme	serine	1592:1597	arg1	inhibitor					1608:1616	serine protease inhibitor Kazal type-5 (SPINK5)	1592:1638	serine protease inhibitor Kazal type-5 (SPINK5)	1592:1638	Meanwhile, GSO treatment was also evaluated for effects on UVB-irradiated hairless mouse skin and HaCaT cells based on levels of serine protease inhibitor Kazal type-5 (SPINK5), trypsin-like kallikrein-related peptidase 5 (KLK5), chymotrypsin-like KLK7, and desmoglein 1 (DSG1).
34562563	6	68	theme	transepidermal	1139:1152	arg1	TEWL					1166:1169	TEWL	1166:1169	TEWL	1166:1169	To evaluate GSO as a skin barrier repair-promoting treatment, skin of UVB-irradiated BALB/c hairless mice was treated with or without GSO then skin samples were evaluated for epidermal thickness, transepidermal water loss (TEWL), and stratum corneum water content.
34562563	6	68	theme	transepidermal	1139:1152	arg1	loss					1160:1163	transepidermal water loss	1139:1163	transepidermal water loss (TEWL)	1139:1170	To evaluate GSO as a skin barrier repair-promoting treatment, skin of UVB-irradiated BALB/c hairless mice was treated with or without GSO then skin samples were evaluated for epidermal thickness, transepidermal water loss (TEWL), and stratum corneum water content.
34562563	9	69	theme	barrier	1794:1800	arg1	damage					1802:1807	UVB-induced skin barrier damage	1777:1807	UVB-induced skin barrier damage manifesting as dryness and desquamation	1777:1847	These proteins are associated with UVB-induced skin barrier damage manifesting as dryness and desquamation.
34562563	2	70	theme	Chinese	391:397	arg1	Panax					349:353	Panax	349:353	Panax ginseng C.A. Meyer	349:372	Panax ginseng C.A. Meyer is a traditional Chinese medicinal herb with known therapeutic benefits for the treatment of skin diseases, including photodamage repair effects and reduction of pigmentation.
34562563	2	70	theme	Chinese	391:397	arg1	herb					409:412	a traditional Chinese medicinal herb	377:412	a traditional Chinese medicinal herb with known therapeutic benefits for the treatment of skin diseases, including photodamage repair effects and reduction of pigmentation	377:547	Panax ginseng C.A. Meyer is a traditional Chinese medicinal herb with known therapeutic benefits for the treatment of skin diseases, including photodamage repair effects and reduction of pigmentation.
34562563	8	71	theme	inhibitor	1608:1616	arg1	levels					1582:1587	levels	1582:1587	levels of serine protease inhibitor Kazal type-5 (SPINK5), trypsin-like kallikrein-related peptidase 5 (KLK5), chymotrypsin-like KLK7, and desmoglein 1 (DSG1)	1582:1739	Meanwhile, GSO treatment was also evaluated for effects on UVB-irradiated hairless mouse skin and HaCaT cells based on levels of serine protease inhibitor Kazal type-5 (SPINK5), trypsin-like kallikrein-related peptidase 5 (KLK5), chymotrypsin-like KLK7, and desmoglein 1 (DSG1).
34562563	2	72	with	herb	409:412	arg1	benefits					437:444	known therapeutic benefits	419:444	known therapeutic benefits for the treatment of skin diseases, including photodamage repair effects and reduction of pigmentation	419:547	Panax ginseng C.A. Meyer is a traditional Chinese medicinal herb with known therapeutic benefits for the treatment of skin diseases, including photodamage repair effects and reduction of pigmentation.
34562563	13	73	theme	increased	2315:2323	arg1	levels					2338:2343	increased DSG1 protein levels	2315:2343	increased DSG1 protein levels due to decreased expression of KLK7	2315:2379	Additionally, GSO treatment led to increased DSG1 protein levels due to decreased expression of KLK7.
34562563	8	74	theme	HaCaT	1561:1565	arg1	cells					1567:1571	UVB-irradiated hairless mouse skin and HaCaT cells	1522:1571	cells	1567:1571	Meanwhile, GSO treatment was also evaluated for effects on UVB-irradiated hairless mouse skin and HaCaT cells based on levels of serine protease inhibitor Kazal type-5 (SPINK5), trypsin-like kallikrein-related peptidase 5 (KLK5), chymotrypsin-like KLK7, and desmoglein 1 (DSG1).
34562563	8	75	from	effects	1511:1517	arg1	skin					1552:1555	UVB-irradiated hairless mouse skin and HaCaT cells	1522:1571	skin	1552:1555	Meanwhile, GSO treatment was also evaluated for effects on UVB-irradiated hairless mouse skin and HaCaT cells based on levels of serine protease inhibitor Kazal type-5 (SPINK5), trypsin-like kallikrein-related peptidase 5 (KLK5), chymotrypsin-like KLK7, and desmoglein 1 (DSG1).
34562563	8	75	from	effects	1511:1517	arg1	cells					1567:1571	UVB-irradiated hairless mouse skin and HaCaT cells	1522:1571	cells	1567:1571	Meanwhile, GSO treatment was also evaluated for effects on UVB-irradiated hairless mouse skin and HaCaT cells based on levels of serine protease inhibitor Kazal type-5 (SPINK5), trypsin-like kallikrein-related peptidase 5 (KLK5), chymotrypsin-like KLK7, and desmoglein 1 (DSG1).
34562563	14	76	theme	HaCaT	2424:2428	arg1	cells					2430:2434	UVB-irradiated HaCaT cells	2409:2434	UVB-irradiated HaCaT cells	2409:2434	In vitro, GSO treatment of UVB-irradiated HaCaT cells led to increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins, while mRNA levels of desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1 and associated protein levels were restored to normal levels.
34562563	10	77	theme	β-glycosidic	2043:2054	arg1	bonds					2056:2060	β-glycosidic bonds	2043:2060	β-glycosidic bonds	2043:2060	RESULTS GSO was shown to consist of oligosaccharides comprised of seven distinct types of monosaccharides with molecular weights of approximately 1 kDa that were covalently linked together via β-glycosidic bonds.
34562563	1	78	theme	homeostatic	274:284	arg1	imbalances					286:295	homeostatic imbalances	274:295	homeostatic imbalances that can trigger atopic dermatitis and anaphylaxis	274:346	ETHNOPHARMACOLOGICAL RELEVANCE Skin barrier dysfunction can lead to water and electrolyte loss, triggering homeostatic imbalances that can trigger atopic dermatitis and anaphylaxis.
34562563	11	79	theme	hairless	2109:2116	arg1	mice					2118:2121	BALB/c hairless mice	2102:2121	BALB/c hairless mice	2102:2121	In vivo, GSO applied to dorsal skin of BALB/c hairless mice attenuated UVB-induced epidermal thickening and moisture loss.
34562563	6	80	theme	water	1193:1197	arg1	content					1199:1205	stratum corneum water content	1177:1205	stratum corneum water content	1177:1205	To evaluate GSO as a skin barrier repair-promoting treatment, skin of UVB-irradiated BALB/c hairless mice was treated with or without GSO then skin samples were evaluated for epidermal thickness, transepidermal water loss (TEWL), and stratum corneum water content.
34562563	14	81	theme	protein	2610:2616	arg1	levels					2618:2623	associated protein levels	2599:2623	associated protein levels	2599:2623	In vitro, GSO treatment of UVB-irradiated HaCaT cells led to increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins, while mRNA levels of desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1 and associated protein levels were restored to normal levels.
34562563	15	82	theme	ginseng	2673:2679	arg1	preparation					2697:2707	A P. ginseng oligosaccharide preparation	2668:2707	A P. ginseng oligosaccharide preparation	2668:2707	CONCLUSION A P. ginseng oligosaccharide preparation repaired UVB-induced skin barrier damage by alleviating skin dryness and desquamation symptoms, highlighting its potential as a natural cosmetic additive that can promote skin barrier repair after UVB exposure.
34562563	3	83	theme	few	559:561	arg1	reports					563:569	few reports	559:569	few reports	559:569	However, few reports exist that describe effectiveness of ginseng active components for repair of skin barrier damage.
34562563	1	84	theme	ETHNOPHARMACOLOGICAL	167:186	arg1	dysfunction					211:221	ETHNOPHARMACOLOGICAL RELEVANCE Skin barrier dysfunction	167:221	ETHNOPHARMACOLOGICAL RELEVANCE Skin barrier dysfunction	167:221	ETHNOPHARMACOLOGICAL RELEVANCE Skin barrier dysfunction can lead to water and electrolyte loss, triggering homeostatic imbalances that can trigger atopic dermatitis and anaphylaxis.
34562563	14	85	theme	GSO	2392:2394	arg1	treatment					2396:2404	GSO treatment	2392:2404	GSO treatment of UVB-irradiated HaCaT cells	2392:2434	In vitro, GSO treatment of UVB-irradiated HaCaT cells led to increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins, while mRNA levels of desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1 and associated protein levels were restored to normal levels.
34562563	0	86	attach	isolated	38:45	arg1	Panax					52:56	Panax	52:56	Panax	52:56	Protective effect of oligosaccharides isolated from Panax ginseng C. A. Meyer against UVB-induced skin barrier damage in BALB/c hairless mice and human keratinocytes.
34562563	0	86	attach	isolated	38:45	arg2	oligosaccharides					21:36	oligosaccharides	21:36	oligosaccharides isolated from Panax	21:56	Protective effect of oligosaccharides isolated from Panax ginseng C. A. Meyer against UVB-induced skin barrier damage in BALB/c hairless mice and human keratinocytes.
34562563	6	87	theme	stratum	1177:1183	arg1	corneum					1185:1191	stratum corneum	1177:1191	stratum corneum water content	1177:1205	To evaluate GSO as a skin barrier repair-promoting treatment, skin of UVB-irradiated BALB/c hairless mice was treated with or without GSO then skin samples were evaluated for epidermal thickness, transepidermal water loss (TEWL), and stratum corneum water content.
34562563	4	88	theme	Ginseng	691:697	arg1	GSO					724:726	GSO	724:726	GSO	724:726	MATERIALS AND METHODS Ginseng oligosaccharide extract (GSO) was prepared from P. ginseng via water extraction followed by ethanol precipitation and resin and gel purification.
34562563	4	88	theme	Ginseng	691:697	arg1	extract					715:721	MATERIALS AND METHODS Ginseng oligosaccharide extract	669:721	MATERIALS AND METHODS Ginseng oligosaccharide extract (GSO)	669:727	MATERIALS AND METHODS Ginseng oligosaccharide extract (GSO) was prepared from P. ginseng via water extraction followed by ethanol precipitation and resin and gel purification.
34562563	8	89	theme	kallikrein-related	1654:1671	arg1	peptidase					1673:1681	trypsin-like kallikrein-related peptidase 5	1641:1683	trypsin-like kallikrein-related peptidase 5 (KLK5)	1641:1690	Meanwhile, GSO treatment was also evaluated for effects on UVB-irradiated hairless mouse skin and HaCaT cells based on levels of serine protease inhibitor Kazal type-5 (SPINK5), trypsin-like kallikrein-related peptidase 5 (KLK5), chymotrypsin-like KLK7, and desmoglein 1 (DSG1).
34562563	8	89	theme	kallikrein-related	1654:1671	arg1	KLK5					1686:1689	KLK5	1686:1689	KLK5	1686:1689	Meanwhile, GSO treatment was also evaluated for effects on UVB-irradiated hairless mouse skin and HaCaT cells based on levels of serine protease inhibitor Kazal type-5 (SPINK5), trypsin-like kallikrein-related peptidase 5 (KLK5), chymotrypsin-like KLK7, and desmoglein 1 (DSG1).
34562563	1	90	theme	Skin	198:201	arg1	dysfunction					211:221	ETHNOPHARMACOLOGICAL RELEVANCE Skin barrier dysfunction	167:221	ETHNOPHARMACOLOGICAL RELEVANCE Skin barrier dysfunction	167:221	ETHNOPHARMACOLOGICAL RELEVANCE Skin barrier dysfunction can lead to water and electrolyte loss, triggering homeostatic imbalances that can trigger atopic dermatitis and anaphylaxis.
34562563	0	91	theme	BALB/c	121:126	arg1	mice					137:140	BALB/c hairless mice	121:140	BALB/c hairless mice	121:140	Protective effect of oligosaccharides isolated from Panax ginseng C. A. Meyer against UVB-induced skin barrier damage in BALB/c hairless mice and human keratinocytes.
34562563	14	92	theme	associated	2599:2608	arg1	levels					2618:2623	associated protein levels	2599:2623	associated protein levels	2599:2623	In vitro, GSO treatment of UVB-irradiated HaCaT cells led to increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins, while mRNA levels of desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1 and associated protein levels were restored to normal levels.
34562563	15	93	theme	skin	2765:2768	arg1	dryness					2770:2776	skin dryness and desquamation symptoms	2765:2802	dryness	2770:2776	CONCLUSION A P. ginseng oligosaccharide preparation repaired UVB-induced skin barrier damage by alleviating skin dryness and desquamation symptoms, highlighting its potential as a natural cosmetic additive that can promote skin barrier repair after UVB exposure.
34562563	13	94	theme	GSO	2294:2296	arg1	treatment					2298:2306	GSO treatment	2294:2306	GSO treatment	2294:2306	Additionally, GSO treatment led to increased DSG1 protein levels due to decreased expression of KLK7.
34562563	14	95	theme	desquamation-related	2536:2555	arg1	SPINK5					2566:2571	SPINK5	2566:2571	SPINK5	2566:2571	In vitro, GSO treatment of UVB-irradiated HaCaT cells led to increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins, while mRNA levels of desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1 and associated protein levels were restored to normal levels.
34562563	14	95	theme	desquamation-related	2536:2555	arg1	DSG1					2590:2593	DSG1	2590:2593	DSG1	2590:2593	In vitro, GSO treatment of UVB-irradiated HaCaT cells led to increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins, while mRNA levels of desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1 and associated protein levels were restored to normal levels.
34562563	14	95	theme	desquamation-related	2536:2555	arg1	KLK7					2580:2583	KLK7	2580:2583	KLK7	2580:2583	In vitro, GSO treatment of UVB-irradiated HaCaT cells led to increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins, while mRNA levels of desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1 and associated protein levels were restored to normal levels.
34562563	14	95	theme	desquamation-related	2536:2555	arg1	proteins					2557:2564	desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1	2536:2593	desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1	2536:2593	In vitro, GSO treatment of UVB-irradiated HaCaT cells led to increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins, while mRNA levels of desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1 and associated protein levels were restored to normal levels.
34562563	14	95	theme	desquamation-related	2536:2555	arg1	KLK5					2574:2577	KLK5	2574:2577	KLK5	2574:2577	In vitro, GSO treatment of UVB-irradiated HaCaT cells led to increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins, while mRNA levels of desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1 and associated protein levels were restored to normal levels.
34562563	14	96	theme	levels	2480:2485	arg1	increases					2443:2451	increases	2443:2451	increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins	2443:2512	In vitro, GSO treatment of UVB-irradiated HaCaT cells led to increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins, while mRNA levels of desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1 and associated protein levels were restored to normal levels.
34562563	1	97	dep	water	235:239	arg1	loss					257:260	loss	257:260	loss	257:260	ETHNOPHARMACOLOGICAL RELEVANCE Skin barrier dysfunction can lead to water and electrolyte loss, triggering homeostatic imbalances that can trigger atopic dermatitis and anaphylaxis.
34562563	7	98	theme	proteins	1386:1393	arg1	levels					1311:1316	levels	1311:1316	levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3)	1311:1460	In addition, UVB-exposed skin samples and HaCaT cells were analyzed to assess GSO treatment effects on levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3).
34562563	10	99	dep	RESULTS	1850:1856	arg1	shown					1866:1870	shown	1866:1870	was shown to consist of oligosaccharides comprised of seven distinct types of monosaccharides with molecular weights of approximately 1 kDa that were covalently linked together via β-glycosidic bonds	1862:2060	RESULTS GSO was shown to consist of oligosaccharides comprised of seven distinct types of monosaccharides with molecular weights of approximately 1 kDa that were covalently linked together via β-glycosidic bonds.
34562563	0	100	theme	human	146:150	arg1	keratinocytes					152:164	human keratinocytes	146:164	human keratinocytes	146:164	Protective effect of oligosaccharides isolated from Panax ginseng C. A. Meyer against UVB-induced skin barrier damage in BALB/c hairless mice and human keratinocytes.
34562563	3	101	theme	active	616:621	arg1	components					623:632	ginseng active components	608:632	ginseng active components	608:632	However, few reports exist that describe effectiveness of ginseng active components for repair of skin barrier damage.
34562563	0	102	from	damage	111:116	arg1	mice					137:140	BALB/c hairless mice	121:140	BALB/c hairless mice	121:140	Protective effect of oligosaccharides isolated from Panax ginseng C. A. Meyer against UVB-induced skin barrier damage in BALB/c hairless mice and human keratinocytes.
34562563	0	102	from	damage	111:116	arg1	keratinocytes					152:164	human keratinocytes	146:164	human keratinocytes	146:164	Protective effect of oligosaccharides isolated from Panax ginseng C. A. Meyer against UVB-induced skin barrier damage in BALB/c hairless mice and human keratinocytes.
34562563	7	103	theme	skin	1373:1376	arg1	involucrin					1421:1430	involucrin	1421:1430	involucrin (IVL)	1421:1436	In addition, UVB-exposed skin samples and HaCaT cells were analyzed to assess GSO treatment effects on levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3).
34562563	7	103	theme	skin	1373:1376	arg1	filaggrin					1404:1412	filaggrin	1404:1412	filaggrin (FLG)	1404:1418	In addition, UVB-exposed skin samples and HaCaT cells were analyzed to assess GSO treatment effects on levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3).
34562563	7	103	theme	skin	1373:1376	arg1	aquaporin-3					1443:1453	aquaporin-3	1443:1453	aquaporin-3 (AQP3)	1443:1460	In addition, UVB-exposed skin samples and HaCaT cells were analyzed to assess GSO treatment effects on levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3).
34562563	7	103	theme	skin	1373:1376	arg1	proteins					1386:1393	other skin barrier proteins	1367:1393	other skin barrier proteins	1367:1393	In addition, UVB-exposed skin samples and HaCaT cells were analyzed to assess GSO treatment effects on levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3).
34562563	15	104	theme	barrier	2735:2741	arg1	damage					2743:2748	UVB-induced skin barrier damage	2718:2748	UVB-induced skin barrier damage	2718:2748	CONCLUSION A P. ginseng oligosaccharide preparation repaired UVB-induced skin barrier damage by alleviating skin dryness and desquamation symptoms, highlighting its potential as a natural cosmetic additive that can promote skin barrier repair after UVB exposure.
34562563	3	105	theme	barrier	653:659	arg1	damage					661:666	skin barrier damage	648:666	skin barrier damage	648:666	However, few reports exist that describe effectiveness of ginseng active components for repair of skin barrier damage.
34562563	8	106	theme	KLK7	1711:1714	arg1	levels					1582:1587	levels	1582:1587	levels of serine protease inhibitor Kazal type-5 (SPINK5), trypsin-like kallikrein-related peptidase 5 (KLK5), chymotrypsin-like KLK7, and desmoglein 1 (DSG1)	1582:1739	Meanwhile, GSO treatment was also evaluated for effects on UVB-irradiated hairless mouse skin and HaCaT cells based on levels of serine protease inhibitor Kazal type-5 (SPINK5), trypsin-like kallikrein-related peptidase 5 (KLK5), chymotrypsin-like KLK7, and desmoglein 1 (DSG1).
34562563	12	107	theme	proteins	2270:2277	arg1	levels					2241:2246	levels	2241:2246	levels of FLG, IVL, and AQP3 proteins	2241:2277	Furthermore, GSO ameliorated UVB-induced reductions of levels of FLG, IVL, and AQP3 proteins.
34562563	10	108	theme	molecular	1961:1969	arg1	weights					1971:1977	molecular weights	1961:1977	molecular weights of approximately 1 kDa that were covalently linked together via β-glycosidic bonds	1961:2060	RESULTS GSO was shown to consist of oligosaccharides comprised of seven distinct types of monosaccharides with molecular weights of approximately 1 kDa that were covalently linked together via β-glycosidic bonds.
34562563	14	109	theme	proteins	2505:2512	arg1	increases					2443:2451	increases	2443:2451	increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins	2443:2512	In vitro, GSO treatment of UVB-irradiated HaCaT cells led to increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins, while mRNA levels of desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1 and associated protein levels were restored to normal levels.
34562563	14	110	theme	mRNA	2475:2478	arg1	levels					2480:2485	AQP3 mRNA levels	2470:2485	AQP3 mRNA levels	2470:2485	In vitro, GSO treatment of UVB-irradiated HaCaT cells led to increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins, while mRNA levels of desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1 and associated protein levels were restored to normal levels.
34562563	12	111	theme	FLG	2251:2253	arg1	levels					2241:2246	levels	2241:2246	levels of FLG, IVL, and AQP3 proteins	2241:2277	Furthermore, GSO ameliorated UVB-induced reductions of levels of FLG, IVL, and AQP3 proteins.
34562563	10	112	theme	monosaccharides	1940:1954	arg1	types					1931:1935	seven distinct types	1916:1935	seven distinct types of monosaccharides with molecular weights of approximately 1 kDa that were covalently linked together via β-glycosidic bonds	1916:2060	RESULTS GSO was shown to consist of oligosaccharides comprised of seven distinct types of monosaccharides with molecular weights of approximately 1 kDa that were covalently linked together via β-glycosidic bonds.
34562563	7	113	theme	UVB-exposed	1221:1231	arg1	samples					1238:1244	UVB-exposed skin samples	1221:1244	UVB-exposed skin samples	1221:1244	In addition, UVB-exposed skin samples and HaCaT cells were analyzed to assess GSO treatment effects on levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3).
34562563	6	114	theme	hairless	1035:1042	arg1	mice					1044:1047	UVB-irradiated BALB/c hairless mice	1013:1047	UVB-irradiated BALB/c hairless mice	1013:1047	To evaluate GSO as a skin barrier repair-promoting treatment, skin of UVB-irradiated BALB/c hairless mice was treated with or without GSO then skin samples were evaluated for epidermal thickness, transepidermal water loss (TEWL), and stratum corneum water content.
34562563	11	115	theme	moisture	2171:2178	arg1	loss					2180:2183	moisture loss	2171:2183	moisture loss	2171:2183	In vivo, GSO applied to dorsal skin of BALB/c hairless mice attenuated UVB-induced epidermal thickening and moisture loss.
34562563	12	116	theme	IVL	2256:2258	arg1	levels					2241:2246	levels	2241:2246	levels of FLG, IVL, and AQP3 proteins	2241:2277	Furthermore, GSO ameliorated UVB-induced reductions of levels of FLG, IVL, and AQP3 proteins.
34562563	10	117	theme	1 kDa	1996:2000	arg1	weights					1971:1977	molecular weights	1961:1977	molecular weights of approximately 1 kDa that were covalently linked together via β-glycosidic bonds	1961:2060	RESULTS GSO was shown to consist of oligosaccharides comprised of seven distinct types of monosaccharides with molecular weights of approximately 1 kDa that were covalently linked together via β-glycosidic bonds.
34562563	0	118	theme	skin	98:101	arg1	damage					111:116	UVB-induced skin barrier damage	86:116	UVB-induced skin barrier damage in BALB/c hairless mice and human keratinocytes	86:164	Protective effect of oligosaccharides isolated from Panax ginseng C. A. Meyer against UVB-induced skin barrier damage in BALB/c hairless mice and human keratinocytes.
34562563	13	119	theme	KLK7	2376:2379	arg1	expression					2362:2371	decreased expression	2352:2371	decreased expression of KLK7	2352:2379	Additionally, GSO treatment led to increased DSG1 protein levels due to decreased expression of KLK7.
34562563	14	120	theme	corresponding	2491:2503	arg1	proteins					2505:2512	corresponding proteins	2491:2512	corresponding proteins	2491:2512	In vitro, GSO treatment of UVB-irradiated HaCaT cells led to increases of FLG, IVL, and AQP3 mRNA levels and corresponding proteins, while mRNA levels of desquamation-related proteins SPINK5, KLK5, KLK7, and DSG1 and associated protein levels were restored to normal levels.
34562563	6	121	theme	UVB-irradiated	1013:1026	arg1	mice					1044:1047	UVB-irradiated BALB/c hairless mice	1013:1047	UVB-irradiated BALB/c hairless mice	1013:1047	To evaluate GSO as a skin barrier repair-promoting treatment, skin of UVB-irradiated BALB/c hairless mice was treated with or without GSO then skin samples were evaluated for epidermal thickness, transepidermal water loss (TEWL), and stratum corneum water content.
34562563	8	122	theme	GSO	1474:1476	arg1	Meanwhile					1463:1471	Meanwhile	1463:1471	Meanwhile	1463:1471	Meanwhile, GSO treatment was also evaluated for effects on UVB-irradiated hairless mouse skin and HaCaT cells based on levels of serine protease inhibitor Kazal type-5 (SPINK5), trypsin-like kallikrein-related peptidase 5 (KLK5), chymotrypsin-like KLK7, and desmoglein 1 (DSG1).
34562563	8	122	theme	GSO	1474:1476	arg1	treatment					1478:1486	GSO treatment	1474:1486	GSO treatment	1474:1486	Meanwhile, GSO treatment was also evaluated for effects on UVB-irradiated hairless mouse skin and HaCaT cells based on levels of serine protease inhibitor Kazal type-5 (SPINK5), trypsin-like kallikrein-related peptidase 5 (KLK5), chymotrypsin-like KLK7, and desmoglein 1 (DSG1).
34562563	15	123	dep	CONCLUSION	2657:2666	arg1	repaired					2709:2716	repaired	2709:2716	repaired	2709:2716	CONCLUSION A P. ginseng oligosaccharide preparation repaired UVB-induced skin barrier damage by alleviating skin dryness and desquamation symptoms, highlighting its potential as a natural cosmetic additive that can promote skin barrier repair after UVB exposure.
34562563	4	124	theme	ethanol	791:797	arg1	precipitation					799:811	ethanol precipitation	791:811	ethanol precipitation	791:811	MATERIALS AND METHODS Ginseng oligosaccharide extract (GSO) was prepared from P. ginseng via water extraction followed by ethanol precipitation and resin and gel purification.
34562563	7	125	theme	envelope	1341:1348	arg1	protein					1355:1361	epidermal cornified envelope (CE) protein	1321:1361	epidermal cornified envelope (CE) protein	1321:1361	In addition, UVB-exposed skin samples and HaCaT cells were analyzed to assess GSO treatment effects on levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3).
34562563	2	126	theme	pigmentation	536:547	arg1	reduction					523:531	reduction	523:531	reduction of pigmentation	523:547	Panax ginseng C.A. Meyer is a traditional Chinese medicinal herb with known therapeutic benefits for the treatment of skin diseases, including photodamage repair effects and reduction of pigmentation.
34562563	2	126	theme	pigmentation	536:547	arg1	effects					511:517	photodamage repair effects	492:517	photodamage repair effects	492:517	Panax ginseng C.A. Meyer is a traditional Chinese medicinal herb with known therapeutic benefits for the treatment of skin diseases, including photodamage repair effects and reduction of pigmentation.
34562563	12	127	theme	levels	2241:2246	arg1	reductions					2227:2236	UVB-induced reductions	2215:2236	UVB-induced reductions of levels of FLG, IVL, and AQP3 proteins	2215:2277	Furthermore, GSO ameliorated UVB-induced reductions of levels of FLG, IVL, and AQP3 proteins.
34562563	5	128	theme	GSO	845:847	arg1	composition					849:859	GSO composition	845:859	GSO composition	845:859	GSO composition and structural characteristics were determined using LC-MS, HPLC, FT-IR, and NMR.
34562563	7	129	theme	epidermal	1321:1329	arg1	CE					1351:1352	CE	1351:1352	CE	1351:1352	In addition, UVB-exposed skin samples and HaCaT cells were analyzed to assess GSO treatment effects on levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3).
34562563	7	129	theme	epidermal	1321:1329	arg1	envelope					1341:1348	epidermal cornified envelope	1321:1348	epidermal cornified envelope (CE) protein	1321:1361	In addition, UVB-exposed skin samples and HaCaT cells were analyzed to assess GSO treatment effects on levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3).
34562563	2	130	theme	skin	467:470	arg1	reduction					523:531	reduction	523:531	reduction of pigmentation	523:547	Panax ginseng C.A. Meyer is a traditional Chinese medicinal herb with known therapeutic benefits for the treatment of skin diseases, including photodamage repair effects and reduction of pigmentation.
34562563	2	130	theme	skin	467:470	arg1	effects					511:517	photodamage repair effects	492:517	photodamage repair effects	492:517	Panax ginseng C.A. Meyer is a traditional Chinese medicinal herb with known therapeutic benefits for the treatment of skin diseases, including photodamage repair effects and reduction of pigmentation.
34562563	2	130	theme	skin	467:470	arg1	diseases					472:479	skin diseases	467:479	skin diseases	467:479	Panax ginseng C.A. Meyer is a traditional Chinese medicinal herb with known therapeutic benefits for the treatment of skin diseases, including photodamage repair effects and reduction of pigmentation.
34562563	4	131	theme	water	762:766	arg1	extraction					768:777	water extraction	762:777	water extraction followed by ethanol precipitation and resin	762:821	MATERIALS AND METHODS Ginseng oligosaccharide extract (GSO) was prepared from P. ginseng via water extraction followed by ethanol precipitation and resin and gel purification.
34562563	2	132	theme	medicinal	399:407	arg1	Panax					349:353	Panax	349:353	Panax ginseng C.A. Meyer	349:372	Panax ginseng C.A. Meyer is a traditional Chinese medicinal herb with known therapeutic benefits for the treatment of skin diseases, including photodamage repair effects and reduction of pigmentation.
34562563	2	132	theme	medicinal	399:407	arg1	herb					409:412	a traditional Chinese medicinal herb	377:412	a traditional Chinese medicinal herb with known therapeutic benefits for the treatment of skin diseases, including photodamage repair effects and reduction of pigmentation	377:547	Panax ginseng C.A. Meyer is a traditional Chinese medicinal herb with known therapeutic benefits for the treatment of skin diseases, including photodamage repair effects and reduction of pigmentation.
34562563	0	133	theme	oligosaccharides	21:36	arg1	effect					11:16	Protective effect	0:16	Protective effect of oligosaccharides isolated from Panax	0:56	Protective effect of oligosaccharides isolated from Panax ginseng C. A. Meyer against UVB-induced skin barrier damage in BALB/c hairless mice and human keratinocytes.
34562563	15	134	theme	barrier	2885:2891	arg1	repair					2893:2898	skin barrier repair	2880:2898	skin barrier repair	2880:2898	CONCLUSION A P. ginseng oligosaccharide preparation repaired UVB-induced skin barrier damage by alleviating skin dryness and desquamation symptoms, highlighting its potential as a natural cosmetic additive that can promote skin barrier repair after UVB exposure.
34562563	11	135	theme	BALB/c	2102:2107	arg1	mice					2118:2121	BALB/c hairless mice	2102:2121	BALB/c hairless mice	2102:2121	In vivo, GSO applied to dorsal skin of BALB/c hairless mice attenuated UVB-induced epidermal thickening and moisture loss.
34562563	1	136	theme	atopic	314:319	arg1	dermatitis					321:330	atopic dermatitis	314:330	atopic dermatitis	314:330	ETHNOPHARMACOLOGICAL RELEVANCE Skin barrier dysfunction can lead to water and electrolyte loss, triggering homeostatic imbalances that can trigger atopic dermatitis and anaphylaxis.
34562563	13	137	theme	DSG1	2325:2328	arg1	levels					2338:2343	increased DSG1 protein levels	2315:2343	increased DSG1 protein levels due to decreased expression of KLK7	2315:2379	Additionally, GSO treatment led to increased DSG1 protein levels due to decreased expression of KLK7.
34562563	2	138	theme	photodamage	492:502	arg1	effects					511:517	photodamage repair effects	492:517	photodamage repair effects	492:517	Panax ginseng C.A. Meyer is a traditional Chinese medicinal herb with known therapeutic benefits for the treatment of skin diseases, including photodamage repair effects and reduction of pigmentation.
34562563	8	139	theme	UVB-irradiated	1522:1535	arg1	skin					1552:1555	UVB-irradiated hairless mouse skin and HaCaT cells	1522:1571	skin	1552:1555	Meanwhile, GSO treatment was also evaluated for effects on UVB-irradiated hairless mouse skin and HaCaT cells based on levels of serine protease inhibitor Kazal type-5 (SPINK5), trypsin-like kallikrein-related peptidase 5 (KLK5), chymotrypsin-like KLK7, and desmoglein 1 (DSG1).
34562563	2	140	theme	therapeutic	425:435	arg1	benefits					437:444	known therapeutic benefits	419:444	known therapeutic benefits for the treatment of skin diseases, including photodamage repair effects and reduction of pigmentation	419:547	Panax ginseng C.A. Meyer is a traditional Chinese medicinal herb with known therapeutic benefits for the treatment of skin diseases, including photodamage repair effects and reduction of pigmentation.
34562563	7	141	theme	GSO	1286:1288	arg1	effects					1300:1306	GSO treatment effects	1286:1306	GSO treatment effects on levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3)	1286:1460	In addition, UVB-exposed skin samples and HaCaT cells were analyzed to assess GSO treatment effects on levels of epidermal cornified envelope (CE) protein and other skin barrier proteins, such as filaggrin (FLG), involucrin (IVL), and aquaporin-3 (AQP3).
34562563	11	142	theme	UVB-induced	2134:2144	arg1	thickening					2156:2165	UVB-induced epidermal thickening	2134:2165	UVB-induced epidermal thickening	2134:2165	In vivo, GSO applied to dorsal skin of BALB/c hairless mice attenuated UVB-induced epidermal thickening and moisture loss.
34562563	8	143	theme	mouse	1546:1550	arg1	skin					1552:1555	UVB-irradiated hairless mouse skin and HaCaT cells	1522:1571	skin	1552:1555	Meanwhile, GSO treatment was also evaluated for effects on UVB-irradiated hairless mouse skin and HaCaT cells based on levels of serine protease inhibitor Kazal type-5 (SPINK5), trypsin-like kallikrein-related peptidase 5 (KLK5), chymotrypsin-like KLK7, and desmoglein 1 (DSG1).
34562563	8	144	theme	protease	1599:1606	arg1	inhibitor					1608:1616	serine protease inhibitor Kazal type-5 (SPINK5)	1592:1638	serine protease inhibitor Kazal type-5 (SPINK5)	1592:1638	Meanwhile, GSO treatment was also evaluated for effects on UVB-irradiated hairless mouse skin and HaCaT cells based on levels of serine protease inhibitor Kazal type-5 (SPINK5), trypsin-like kallikrein-related peptidase 5 (KLK5), chymotrypsin-like KLK7, and desmoglein 1 (DSG1).
34562563	6	145	theme	water	1154:1158	arg1	TEWL					1166:1169	TEWL	1166:1169	TEWL	1166:1169	To evaluate GSO as a skin barrier repair-promoting treatment, skin of UVB-irradiated BALB/c hairless mice was treated with or without GSO then skin samples were evaluated for epidermal thickness, transepidermal water loss (TEWL), and stratum corneum water content.
34562563	6	145	theme	water	1154:1158	arg1	loss					1160:1163	transepidermal water loss	1139:1163	transepidermal water loss (TEWL)	1139:1170	To evaluate GSO as a skin barrier repair-promoting treatment, skin of UVB-irradiated BALB/c hairless mice was treated with or without GSO then skin samples were evaluated for epidermal thickness, transepidermal water loss (TEWL), and stratum corneum water content.
34562563	9	146	theme	skin	1789:1792	arg1	damage					1802:1807	UVB-induced skin barrier damage	1777:1807	UVB-induced skin barrier damage manifesting as dryness and desquamation	1777:1847	These proteins are associated with UVB-induced skin barrier damage manifesting as dryness and desquamation.
34562563	8	147	theme	Kazal	1618:1622	arg1	SPINK5					1632:1637	SPINK5	1632:1637	SPINK5	1632:1637	Meanwhile, GSO treatment was also evaluated for effects on UVB-irradiated hairless mouse skin and HaCaT cells based on levels of serine protease inhibitor Kazal type-5 (SPINK5), trypsin-like kallikrein-related peptidase 5 (KLK5), chymotrypsin-like KLK7, and desmoglein 1 (DSG1).
34562563	8	147	theme	Kazal	1618:1622	arg1	type-5					1624:1629	Kazal type-5	1618:1629	serine protease inhibitor Kazal type-5 (SPINK5)	1592:1638	Meanwhile, GSO treatment was also evaluated for effects on UVB-irradiated hairless mouse skin and HaCaT cells based on levels of serine protease inhibitor Kazal type-5 (SPINK5), trypsin-like kallikrein-related peptidase 5 (KLK5), chymotrypsin-like KLK7, and desmoglein 1 (DSG1).
34562563	2	148	theme	traditional	379:389	arg1	Panax					349:353	Panax	349:353	Panax ginseng C.A. Meyer	349:372	Panax ginseng C.A. Meyer is a traditional Chinese medicinal herb with known therapeutic benefits for the treatment of skin diseases, including photodamage repair effects and reduction of pigmentation.
34562563	2	148	theme	traditional	379:389	arg1	herb					409:412	a traditional Chinese medicinal herb	377:412	a traditional Chinese medicinal herb with known therapeutic benefits for the treatment of skin diseases, including photodamage repair effects and reduction of pigmentation	377:547	Panax ginseng C.A. Meyer is a traditional Chinese medicinal herb with known therapeutic benefits for the treatment of skin diseases, including photodamage repair effects and reduction of pigmentation.
33360072	4	0	theme	C.	1044:1045	arg1	strain					1053:1058	the C. rubra strain	1040:1058	the C. rubra strain	1040:1058	The sizes of whole genome sequences generated for the isolates and the C. rubra strain ranged from 11.20 to 12.80 Mbp with corresponding in silico DNA G+C values of 69.9-70.0%.
33360072	6	1	theme	phenotypic	1417:1426	arg1	features					1428:1435	many chemotaxonomic, cultural and phenotypic features	1383:1435	many chemotaxonomic, cultural and phenotypic features	1383:1435	Isolates NF3 and NH11T belong to the same species as they have identical 16S rRNA gene sequences, share many chemotaxonomic, cultural and phenotypic features and show very high average nucleotide identity (ANI) and digital DNA:DNA relatedness (dDDH) similarities.
33360072	5	2	theme	acidiphila	1174:1183	arg1	strains					1198:1204	the C. acidiphila and C. rubra strains	1167:1204	the C. acidiphila and C. rubra strains	1167:1204	The isolates and the C. acidiphila and C. rubra strains formed a well supported branch in the actinobacterial phylogenomic tree.
33360072	9	3	theme	111109T	1965:1971	arg1	NH11T					1953:1957	NH11T	1953:1957	NH11T (=DSM 111109T =PCM 3046T)	1953:1983	nov., the type strain is NH11T (=DSM 111109T =PCM 3046T).
33360072	9	3	theme	111109T	1965:1971	arg1	3046T					1978:1982	=DSM 111109T =PCM 3046T	1960:1982	=DSM 111109T =PCM 3046T	1960:1982	nov., the type strain is NH11T (=DSM 111109T =PCM 3046T).
33360072	5	4	from	branch	1230:1235	arg1	tree					1273:1276	the actinobacterial phylogenomic tree	1240:1276	the actinobacterial phylogenomic tree	1240:1276	The isolates and the C. acidiphila and C. rubra strains formed a well supported branch in the actinobacterial phylogenomic tree.
33360072	3	5	theme	diagnostic	537:546	arg1	sugar					563:567	the diagnostic whole-organism sugar	533:567	the diagnostic whole-organism sugar	533:567	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	6	6	theme	DNA	1502:1504	arg1	similarities					1529:1540	digital DNA:DNA relatedness (dDDH) similarities	1494:1540	digital DNA:DNA relatedness (dDDH) similarities	1494:1540	Isolates NF3 and NH11T belong to the same species as they have identical 16S rRNA gene sequences, share many chemotaxonomic, cultural and phenotypic features and show very high average nucleotide identity (ANI) and digital DNA:DNA relatedness (dDDH) similarities.
33360072	12	7	theme	bacteria	2364:2371	arg1	growth					2340:2345	the growth	2336:2345	the growth of Gram-positive bacteria and wheat pathogens belonging to the genus Fusarium	2336:2423	In vitro screens showed that the isolates inhibited the growth of Gram-positive bacteria and wheat pathogens belonging to the genus Fusarium.
33360072	0	8	theme	Genomic-based	0:12	arg1	classification					14:27	Genomic-based classification	0:27	Genomic-based classification of Catenulispora pinisilvae sp	0:58	Genomic-based classification of Catenulispora pinisilvae sp.
33360072	3	9	with	iso-C16:0	681:689	arg1	units					640:644	nine isoprenoid units	624:644	nine isoprenoid units as the predominant isoprenologues	624:678	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	6	10	dep	Isolates	1279:1286	arg1	NF3					1288:1290	NF3	1288:1290	NF3	1288:1290	Isolates NF3 and NH11T belong to the same species as they have identical 16S rRNA gene sequences, share many chemotaxonomic, cultural and phenotypic features and show very high average nucleotide identity (ANI) and digital DNA:DNA relatedness (dDDH) similarities.
33360072	6	10	dep	Isolates	1279:1286	arg1	Isolates					1279:1286	Isolates NF3 and NH11T	1279:1300	Isolates NF3 and NH11T	1279:1300	Isolates NF3 and NH11T belong to the same species as they have identical 16S rRNA gene sequences, share many chemotaxonomic, cultural and phenotypic features and show very high average nucleotide identity (ANI) and digital DNA:DNA relatedness (dDDH) similarities.
33360072	6	10	dep	Isolates	1279:1286	arg1	NH11T					1296:1300	NH11T	1296:1300	NH11T	1296:1300	Isolates NF3 and NH11T belong to the same species as they have identical 16S rRNA gene sequences, share many chemotaxonomic, cultural and phenotypic features and show very high average nucleotide identity (ANI) and digital DNA:DNA relatedness (dDDH) similarities.
33360072	4	11	theme	G+C	1124:1126	arg1	values					1128:1133	corresponding in silico DNA G+C values	1096:1133	corresponding in silico DNA G+C values of 69.9-70.0%	1096:1147	The sizes of whole genome sequences generated for the isolates and the C. rubra strain ranged from 11.20 to 12.80 Mbp with corresponding in silico DNA G+C values of 69.9-70.0%.
33360072	3	12	theme	supported	749:757	arg1	clade					759:763	a well supported clade	742:763	a well supported clade within the Catenulispora 16S rRNA gene tree	742:807	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	7	13	theme	phylogenomic	1588:1599	arg1	neighbours					1601:1610	their closest phylogenomic neighbours	1574:1610	their closest phylogenomic neighbours	1574:1610	They can be distinguished from their closest phylogenomic neighbours by using a combination of chemotaxonomic and phenotypic properties and by ANI and dDDH values well below the thresholds of these metrics used to assign closely related strains to different species.
33360072	7	14	theme	metrics	1741:1747	arg1	thresholds					1721:1730	the thresholds	1717:1730	the thresholds of these metrics used to assign closely related strains to different species	1717:1807	They can be distinguished from their closest phylogenomic neighbours by using a combination of chemotaxonomic and phenotypic properties and by ANI and dDDH values well below the thresholds of these metrics used to assign closely related strains to different species.
33360072	4	15	theme	corresponding	1096:1108	arg1	values					1128:1133	corresponding in silico DNA G+C values	1096:1133	corresponding in silico DNA G+C values of 69.9-70.0%	1096:1147	The sizes of whole genome sequences generated for the isolates and the C. rubra strain ranged from 11.20 to 12.80 Mbp with corresponding in silico DNA G+C values of 69.9-70.0%.
33360072	7	16	theme	dDDH	1694:1697	arg1	values					1699:1704	ANI and dDDH values	1686:1704	ANI and dDDH values well below the thresholds of these metrics used to assign closely related strains to different species	1686:1807	They can be distinguished from their closest phylogenomic neighbours by using a combination of chemotaxonomic and phenotypic properties and by ANI and dDDH values well below the thresholds of these metrics used to assign closely related strains to different species.
33360072	3	17	theme	Catenulispora	776:788	arg1	tree					804:807	the Catenulispora 16S rRNA gene tree	772:807	the Catenulispora 16S rRNA gene tree	772:807	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	3	18	theme	octa-hydrogenated	588:604	arg1	menaquinones					606:617	octa-hydrogenated menaquinones	588:617	octa-hydrogenated menaquinones	588:617	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	4	19	dep	in	1110:1111	arg1	silico					1113:1118	silico	1113:1118	silico	1113:1118	The sizes of whole genome sequences generated for the isolates and the C. rubra strain ranged from 11.20 to 12.80 Mbp with corresponding in silico DNA G+C values of 69.9-70.0%.
33360072	8	20	theme	new	1872:1874	arg1	species					1890:1896	a new Catenulispora species	1870:1896	a new Catenulispora species	1870:1896	Consequently, we propose that the isolates be classified as a new Catenulispora species, Catenulispora pinisilvae sp.
33360072	8	20	theme	new	1872:1874	arg1	isolates					1844:1851	the isolates	1840:1851	the isolates	1840:1851	Consequently, we propose that the isolates be classified as a new Catenulispora species, Catenulispora pinisilvae sp.
33360072	3	21	theme	Catenulispora	863:875	arg1	44948T					887:892	Catenulispora rubra DSM 44948T	863:892	Catenulispora rubra DSM 44948T	863:892	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	10	22	theme	present	2061:2067	arg1	study					2069:2073	the present study	2057:2073	the present study	2057:2073	An emended description is given for C. rubra based on data acquired in the present study.
33360072	5	23	theme	actinobacterial	1244:1258	arg1	tree					1273:1276	the actinobacterial phylogenomic tree	1240:1276	the actinobacterial phylogenomic tree	1240:1276	The isolates and the C. acidiphila and C. rubra strains formed a well supported branch in the actinobacterial phylogenomic tree.
33360072	3	24	theme	isoprenoid	629:638	arg1	units					640:644	nine isoprenoid units	624:644	nine isoprenoid units as the predominant isoprenologues	624:678	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	7	25	theme	ANI	1686:1688	arg1	values					1699:1704	ANI and dDDH values	1686:1704	ANI and dDDH values well below the thresholds of these metrics used to assign closely related strains to different species	1686:1807	They can be distinguished from their closest phylogenomic neighbours by using a combination of chemotaxonomic and phenotypic properties and by ANI and dDDH values well below the thresholds of these metrics used to assign closely related strains to different species.
33360072	11	26	theme	isolates	2113:2120	arg1	genomes					2098:2104	the draft genomes	2088:2104	the draft genomes of the isolates and the C. acidiphila and C. rubra strains	2088:2163	Analyses of the draft genomes of the isolates and the C. acidiphila and C. rubra strains revealed the presence of many biosynthetic gene clusters with the potential to synthesize novel drug-like metabolites.
33360072	3	27	theme	rRNA	794:797	arg1	tree					804:807	the Catenulispora 16S rRNA gene tree	772:807	the Catenulispora 16S rRNA gene tree	772:807	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	6	28	theme	16S	1352:1354	arg1	sequences					1366:1374	identical 16S rRNA gene sequences	1342:1374	identical 16S rRNA gene sequences	1342:1374	Isolates NF3 and NH11T belong to the same species as they have identical 16S rRNA gene sequences, share many chemotaxonomic, cultural and phenotypic features and show very high average nucleotide identity (ANI) and digital DNA:DNA relatedness (dDDH) similarities.
33360072	6	29	theme	DNA	1506:1508	arg1	similarities					1529:1540	digital DNA:DNA relatedness (dDDH) similarities	1494:1540	digital DNA:DNA relatedness (dDDH) similarities	1494:1540	Isolates NF3 and NH11T belong to the same species as they have identical 16S rRNA gene sequences, share many chemotaxonomic, cultural and phenotypic features and show very high average nucleotide identity (ANI) and digital DNA:DNA relatedness (dDDH) similarities.
33360072	4	30	theme	whole	986:990	arg1	sequences					999:1007	whole genome sequences	986:1007	whole genome sequences generated for the isolates and the C. rubra strain	986:1058	The sizes of whole genome sequences generated for the isolates and the C. rubra strain ranged from 11.20 to 12.80 Mbp with corresponding in silico DNA G+C values of 69.9-70.0%.
33360072	4	31	theme	%	1147:1147	arg1	values					1128:1133	corresponding in silico DNA G+C values	1096:1133	corresponding in silico DNA G+C values of 69.9-70.0%	1096:1147	The sizes of whole genome sequences generated for the isolates and the C. rubra strain ranged from 11.20 to 12.80 Mbp with corresponding in silico DNA G+C values of 69.9-70.0%.
33360072	5	32	theme	supported	1220:1228	arg1	branch					1230:1235	a well supported branch	1213:1235	a well supported branch in the actinobacterial phylogenomic tree	1213:1276	The isolates and the C. acidiphila and C. rubra strains formed a well supported branch in the actinobacterial phylogenomic tree.
33360072	3	33	theme	acidiphila	837:846	arg1	44928T					852:857	Catenulispora acidiphila DSM 44928T	823:857	Catenulispora acidiphila DSM 44928T	823:857	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	1	34	theme	Catenulispora	158:170	arg1	description					143:153	emended description	135:153	emended description of Catenulispora rubra	135:176	nov., novel actinobacteria isolated from a pine forest soil in Poland and emended description of Catenulispora rubra.
33360072	1	34	theme	Catenulispora	158:170	arg1	soil					116:119	a pine forest soil	102:119	a pine forest soil in Poland	102:129	nov., novel actinobacteria isolated from a pine forest soil in Poland and emended description of Catenulispora rubra.
33360072	3	35	theme	DSM	883:885	arg1	44948T					887:892	Catenulispora rubra DSM 44948T	863:892	Catenulispora rubra DSM 44948T	863:892	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	11	36	theme	acidiphila	2133:2142	arg1	strains					2157:2163	the C. acidiphila and C. rubra strains	2126:2163	the C. acidiphila and C. rubra strains	2126:2163	Analyses of the draft genomes of the isolates and the C. acidiphila and C. rubra strains revealed the presence of many biosynthetic gene clusters with the potential to synthesize novel drug-like metabolites.
33360072	2	37	theme	forest	243:248	arg1	soil					250:253	a pine forest soil	236:253	a pine forest soil	236:253	Two actinobacteria, strains NF3 and NH11T, isolated from a pine forest soil, near Torun, Poland were examined for diverse chemotaxonomic and morphological properties that placed them in the genus Catenulispora.
33360072	3	38	with	hexa-	578:582	arg1	units					640:644	nine isoprenoid units	624:644	nine isoprenoid units as the predominant isoprenologues	624:678	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	1	39	theme	novel	67:71	arg1	actinobacteria					73:86	novel actinobacteria	67:86	novel actinobacteria	67:86	nov., novel actinobacteria isolated from a pine forest soil in Poland and emended description of Catenulispora rubra.
33360072	1	39	theme	novel	67:71	arg1	nov.					61:64	nov.	61:64	nov.	61:64	nov., novel actinobacteria isolated from a pine forest soil in Poland and emended description of Catenulispora rubra.
33360072	7	40	theme	chemotaxonomic	1638:1651	arg1	properties					1668:1677	chemotaxonomic and phenotypic properties	1638:1677	chemotaxonomic and phenotypic properties	1638:1677	They can be distinguished from their closest phylogenomic neighbours by using a combination of chemotaxonomic and phenotypic properties and by ANI and dDDH values well below the thresholds of these metrics used to assign closely related strains to different species.
33360072	8	41	theme	pinisilvae	1913:1922	arg1	sp					1924:1925	pinisilvae sp	1913:1925	pinisilvae sp	1913:1925	Consequently, we propose that the isolates be classified as a new Catenulispora species, Catenulispora pinisilvae sp.
33360072	11	42	theme	drug-like	2261:2269	arg1	metabolites					2271:2281	novel drug-like metabolites	2255:2281	novel drug-like metabolites	2255:2281	Analyses of the draft genomes of the isolates and the C. acidiphila and C. rubra strains revealed the presence of many biosynthetic gene clusters with the potential to synthesize novel drug-like metabolites.
33360072	3	43	theme	stable	428:433	arg1	mycelium					435:442	an extensively branched stable mycelium	404:442	an extensively branched stable mycelium	404:442	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	6	44	theme	same	1316:1319	arg1	species					1321:1327	the same species	1312:1327	the same species	1312:1327	Isolates NF3 and NH11T belong to the same species as they have identical 16S rRNA gene sequences, share many chemotaxonomic, cultural and phenotypic features and show very high average nucleotide identity (ANI) and digital DNA:DNA relatedness (dDDH) similarities.
33360072	11	45	theme	draft	2092:2096	arg1	genomes					2098:2104	the draft genomes	2088:2104	the draft genomes of the isolates and the C. acidiphila and C. rubra strains	2088:2163	Analyses of the draft genomes of the isolates and the C. acidiphila and C. rubra strains revealed the presence of many biosynthetic gene clusters with the potential to synthesize novel drug-like metabolites.
33360072	4	46	dep	12.80	1081:1085	arg1	to					1078:1079	to	1078:1079	to	1078:1079	The sizes of whole genome sequences generated for the isolates and the C. rubra strain ranged from 11.20 to 12.80 Mbp with corresponding in silico DNA G+C values of 69.9-70.0%.
33360072	3	47	with	menaquinones	606:617	arg1	units					640:644	nine isoprenoid units	624:644	nine isoprenoid units as the predominant isoprenologues	624:678	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	3	48	with	sugar	563:567	arg1	units					640:644	nine isoprenoid units	624:644	nine isoprenoid units as the predominant isoprenologues	624:678	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	1	49	from	soil	116:119	arg1	Poland					124:129	Poland	124:129	Poland	124:129	nov., novel actinobacteria isolated from a pine forest soil in Poland and emended description of Catenulispora rubra.
33360072	11	50	theme	strains	2157:2163	arg1	genomes					2098:2104	the draft genomes	2088:2104	the draft genomes of the isolates and the C. acidiphila and C. rubra strains	2088:2163	Analyses of the draft genomes of the isolates and the C. acidiphila and C. rubra strains revealed the presence of many biosynthetic gene clusters with the potential to synthesize novel drug-like metabolites.
33360072	3	51	theme	LL-diaminopimelic	455:471	arg1	acid					493:496	the diamino acid	481:496	the diamino acid of the peptidoglycan	481:517	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	3	51	theme	LL-diaminopimelic	455:471	arg1	acid					473:476	LL-diaminopimelic acid	455:476	LL-diaminopimelic acid	455:476	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	6	52	theme	many	1383:1386	arg1	features					1428:1435	many chemotaxonomic, cultural and phenotypic features	1383:1435	many chemotaxonomic, cultural and phenotypic features	1383:1435	Isolates NF3 and NH11T belong to the same species as they have identical 16S rRNA gene sequences, share many chemotaxonomic, cultural and phenotypic features and show very high average nucleotide identity (ANI) and digital DNA:DNA relatedness (dDDH) similarities.
33360072	1	53	theme	forest	109:114	arg1	soil					116:119	a pine forest soil	102:119	a pine forest soil in Poland	102:129	nov., novel actinobacteria isolated from a pine forest soil in Poland and emended description of Catenulispora rubra.
33360072	4	54	theme	sequences	999:1007	arg1	sizes					977:981	The sizes	973:981	The sizes of whole genome sequences generated for the isolates and the C. rubra strain	973:1058	The sizes of whole genome sequences generated for the isolates and the C. rubra strain ranged from 11.20 to 12.80 Mbp with corresponding in silico DNA G+C values of 69.9-70.0%.
33360072	3	55	theme	predominant	653:663	arg1	isoprenologues					665:678	the predominant isoprenologues	649:678	the predominant isoprenologues	649:678	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	0	56	theme	pinisilvae	46:55	arg1	sp					57:58	Catenulispora pinisilvae sp	32:58	Catenulispora pinisilvae sp	32:58	Genomic-based classification of Catenulispora pinisilvae sp.
33360072	11	57	theme	genomes	2098:2104	arg1	Analyses					2076:2083	Analyses	2076:2083	Analyses of the draft genomes of the isolates and the C. acidiphila and C. rubra strains	2076:2163	Analyses of the draft genomes of the isolates and the C. acidiphila and C. rubra strains revealed the presence of many biosynthetic gene clusters with the potential to synthesize novel drug-like metabolites.
33360072	7	58	theme	different	1791:1799	arg1	species					1801:1807	different species	1791:1807	different species	1791:1807	They can be distinguished from their closest phylogenomic neighbours by using a combination of chemotaxonomic and phenotypic properties and by ANI and dDDH values well below the thresholds of these metrics used to assign closely related strains to different species.
33360072	3	59	theme	diamino	485:491	arg1	acid					493:496	the diamino acid	481:496	the diamino acid of the peptidoglycan	481:517	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	3	59	theme	diamino	485:491	arg1	acid					473:476	LL-diaminopimelic acid	455:476	LL-diaminopimelic acid	455:476	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	6	60	theme	gene	1361:1364	arg1	sequences					1366:1374	identical 16S rRNA gene sequences	1342:1374	identical 16S rRNA gene sequences	1342:1374	Isolates NF3 and NH11T belong to the same species as they have identical 16S rRNA gene sequences, share many chemotaxonomic, cultural and phenotypic features and show very high average nucleotide identity (ANI) and digital DNA:DNA relatedness (dDDH) similarities.
33360072	3	61	theme	%	956:956	arg1	latter					933:938	latter	933:938	latter	933:938	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	10	62	theme	emended	1989:1995	arg1	description					1997:2007	An emended description	1986:2007	An emended description	1986:2007	An emended description is given for C. rubra based on data acquired in the present study.
33360072	2	63	theme	chemotaxonomic	301:314	arg1	properties					334:343	diverse chemotaxonomic and morphological properties	293:343	diverse chemotaxonomic and morphological properties that placed them in the genus Catenulispora	293:387	Two actinobacteria, strains NF3 and NH11T, isolated from a pine forest soil, near Torun, Poland were examined for diverse chemotaxonomic and morphological properties that placed them in the genus Catenulispora.
33360072	6	64	theme	high	1451:1454	arg1	ANI					1485:1487	ANI	1485:1487	ANI	1485:1487	Isolates NF3 and NH11T belong to the same species as they have identical 16S rRNA gene sequences, share many chemotaxonomic, cultural and phenotypic features and show very high average nucleotide identity (ANI) and digital DNA:DNA relatedness (dDDH) similarities.
33360072	6	64	theme	high	1451:1454	arg1	identity					1475:1482	very high average nucleotide identity	1446:1482	very high average nucleotide identity (ANI)	1446:1488	Isolates NF3 and NH11T belong to the same species as they have identical 16S rRNA gene sequences, share many chemotaxonomic, cultural and phenotypic features and show very high average nucleotide identity (ANI) and digital DNA:DNA relatedness (dDDH) similarities.
33360072	5	65	theme	rubra	1192:1196	arg1	strains					1198:1204	the C. acidiphila and C. rubra strains	1167:1204	the C. acidiphila and C. rubra strains	1167:1204	The isolates and the C. acidiphila and C. rubra strains formed a well supported branch in the actinobacterial phylogenomic tree.
33360072	11	66	theme	biosynthetic	2195:2206	arg1	clusters					2213:2220	many biosynthetic gene clusters	2190:2220	many biosynthetic gene clusters	2190:2220	Analyses of the draft genomes of the isolates and the C. acidiphila and C. rubra strains revealed the presence of many biosynthetic gene clusters with the potential to synthesize novel drug-like metabolites.
33360072	12	67	theme	In	2284:2285	arg1	screens					2293:2299	In vitro screens	2284:2299	In vitro screens	2284:2299	In vitro screens showed that the isolates inhibited the growth of Gram-positive bacteria and wheat pathogens belonging to the genus Fusarium.
33360072	6	68	theme	chemotaxonomic	1388:1401	arg1	features					1428:1435	many chemotaxonomic, cultural and phenotypic features	1383:1435	many chemotaxonomic, cultural and phenotypic features	1383:1435	Isolates NF3 and NH11T belong to the same species as they have identical 16S rRNA gene sequences, share many chemotaxonomic, cultural and phenotypic features and show very high average nucleotide identity (ANI) and digital DNA:DNA relatedness (dDDH) similarities.
33360072	3	69	theme	peptidoglycan	505:517	arg1	acid					493:496	the diamino acid	481:496	the diamino acid of the peptidoglycan	481:517	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	3	69	theme	peptidoglycan	505:517	arg1	acid					473:476	LL-diaminopimelic acid	455:476	LL-diaminopimelic acid	455:476	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	3	70	theme	fatty	718:722	arg1	acids					724:728	major fatty acids	712:728	major fatty acids	712:728	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	6	71	contain	have	1337:1340	arg1	they					1332:1335	they	1332:1335	they	1332:1335	Isolates NF3 and NH11T belong to the same species as they have identical 16S rRNA gene sequences, share many chemotaxonomic, cultural and phenotypic features and show very high average nucleotide identity (ANI) and digital DNA:DNA relatedness (dDDH) similarities.
33360072	6	71	contain	have	1337:1340	arg2	sequences					1366:1374	identical 16S rRNA gene sequences	1342:1374	identical 16S rRNA gene sequences	1342:1374	Isolates NF3 and NH11T belong to the same species as they have identical 16S rRNA gene sequences, share many chemotaxonomic, cultural and phenotypic features and show very high average nucleotide identity (ANI) and digital DNA:DNA relatedness (dDDH) similarities.
33360072	9	72	theme	type	1938:1941	arg1	strain					1943:1948	the type strain	1934:1948	the type strain	1934:1948	nov., the type strain is NH11T (=DSM 111109T =PCM 3046T).
33360072	9	72	theme	type	1938:1941	arg1	nov.					1928:1931	nov.	1928:1931	nov.	1928:1931	nov., the type strain is NH11T (=DSM 111109T =PCM 3046T).
33360072	4	73	theme	DNA	1120:1122	arg1	values					1128:1133	corresponding in silico DNA G+C values	1096:1133	corresponding in silico DNA G+C values of 69.9-70.0%	1096:1147	The sizes of whole genome sequences generated for the isolates and the C. rubra strain ranged from 11.20 to 12.80 Mbp with corresponding in silico DNA G+C values of 69.9-70.0%.
33360072	11	74	theme	rubra	2151:2155	arg1	strains					2157:2163	the C. acidiphila and C. rubra strains	2126:2163	the C. acidiphila and C. rubra strains	2126:2163	Analyses of the draft genomes of the isolates and the C. acidiphila and C. rubra strains revealed the presence of many biosynthetic gene clusters with the potential to synthesize novel drug-like metabolites.
33360072	2	75	theme	morphological	320:332	arg1	properties					334:343	diverse chemotaxonomic and morphological properties	293:343	diverse chemotaxonomic and morphological properties that placed them in the genus Catenulispora	293:387	Two actinobacteria, strains NF3 and NH11T, isolated from a pine forest soil, near Torun, Poland were examined for diverse chemotaxonomic and morphological properties that placed them in the genus Catenulispora.
33360072	6	76	theme	digital	1494:1500	arg1	similarities					1529:1540	digital DNA:DNA relatedness (dDDH) similarities	1494:1540	digital DNA:DNA relatedness (dDDH) similarities	1494:1540	Isolates NF3 and NH11T belong to the same species as they have identical 16S rRNA gene sequences, share many chemotaxonomic, cultural and phenotypic features and show very high average nucleotide identity (ANI) and digital DNA:DNA relatedness (dDDH) similarities.
33360072	8	77	dep	sp	1924:1925	arg1	Catenulispora					1899:1911	Catenulispora	1899:1911	Catenulispora	1899:1911	Consequently, we propose that the isolates be classified as a new Catenulispora species, Catenulispora pinisilvae sp.
33360072	12	78	theme	genus	2410:2414	arg1	Fusarium					2416:2423	the genus Fusarium	2406:2423	the genus Fusarium	2406:2423	In vitro screens showed that the isolates inhibited the growth of Gram-positive bacteria and wheat pathogens belonging to the genus Fusarium.
33360072	7	79	theme	closest	1580:1586	arg1	neighbours					1601:1610	their closest phylogenomic neighbours	1574:1610	their closest phylogenomic neighbours	1574:1610	They can be distinguished from their closest phylogenomic neighbours by using a combination of chemotaxonomic and phenotypic properties and by ANI and dDDH values well below the thresholds of these metrics used to assign closely related strains to different species.
33360072	3	80	theme	whole-organism	548:561	arg1	sugar					563:567	the diagnostic whole-organism sugar	533:567	the diagnostic whole-organism sugar	533:567	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	3	81	contain	contained	445:453	arg2	acid					473:476	LL-diaminopimelic acid	455:476	LL-diaminopimelic acid	455:476	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	3	81	contain	contained	445:453	arg1	They					390:393	They	390:393	They	390:393	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	3	81	contain	contained	445:453	arg2	acid					493:496	the diamino acid	481:496	the diamino acid of the peptidoglycan	481:517	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	4	82	theme	in	1110:1111	arg1	values					1128:1133	corresponding in silico DNA G+C values	1096:1133	corresponding in silico DNA G+C values of 69.9-70.0%	1096:1147	The sizes of whole genome sequences generated for the isolates and the C. rubra strain ranged from 11.20 to 12.80 Mbp with corresponding in silico DNA G+C values of 69.9-70.0%.
33360072	7	83	theme	phenotypic	1657:1666	arg1	properties					1668:1677	chemotaxonomic and phenotypic properties	1638:1677	chemotaxonomic and phenotypic properties	1638:1677	They can be distinguished from their closest phylogenomic neighbours by using a combination of chemotaxonomic and phenotypic properties and by ANI and dDDH values well below the thresholds of these metrics used to assign closely related strains to different species.
33360072	6	84	theme	nucleotide	1464:1473	arg1	ANI					1485:1487	ANI	1485:1487	ANI	1485:1487	Isolates NF3 and NH11T belong to the same species as they have identical 16S rRNA gene sequences, share many chemotaxonomic, cultural and phenotypic features and show very high average nucleotide identity (ANI) and digital DNA:DNA relatedness (dDDH) similarities.
33360072	6	84	theme	nucleotide	1464:1473	arg1	identity					1475:1482	very high average nucleotide identity	1446:1482	very high average nucleotide identity (ANI)	1446:1488	Isolates NF3 and NH11T belong to the same species as they have identical 16S rRNA gene sequences, share many chemotaxonomic, cultural and phenotypic features and show very high average nucleotide identity (ANI) and digital DNA:DNA relatedness (dDDH) similarities.
33360072	11	85	theme	clusters	2213:2220	arg1	presence					2178:2185	the presence	2174:2185	the presence of many biosynthetic gene clusters	2174:2220	Analyses of the draft genomes of the isolates and the C. acidiphila and C. rubra strains revealed the presence of many biosynthetic gene clusters with the potential to synthesize novel drug-like metabolites.
33360072	3	86	theme	16S	790:792	arg1	tree					804:807	the Catenulispora 16S rRNA gene tree	772:807	the Catenulispora 16S rRNA gene tree	772:807	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	8	87	theme	Catenulispora	1876:1888	arg1	species					1890:1896	a new Catenulispora species	1870:1896	a new Catenulispora species	1870:1896	Consequently, we propose that the isolates be classified as a new Catenulispora species, Catenulispora pinisilvae sp.
33360072	8	87	theme	Catenulispora	1876:1888	arg1	isolates					1844:1851	the isolates	1840:1851	the isolates	1840:1851	Consequently, we propose that the isolates be classified as a new Catenulispora species, Catenulispora pinisilvae sp.
33360072	6	88	theme	dDDH	1523:1526	arg1	similarities					1529:1540	digital DNA:DNA relatedness (dDDH) similarities	1494:1540	digital DNA:DNA relatedness (dDDH) similarities	1494:1540	Isolates NF3 and NH11T belong to the same species as they have identical 16S rRNA gene sequences, share many chemotaxonomic, cultural and phenotypic features and show very high average nucleotide identity (ANI) and digital DNA:DNA relatedness (dDDH) similarities.
33360072	3	89	theme	rubra	877:881	arg1	44948T					887:892	Catenulispora rubra DSM 44948T	863:892	Catenulispora rubra DSM 44948T	863:892	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	11	90	theme	many	2190:2193	arg1	clusters					2213:2220	many biosynthetic gene clusters	2190:2220	many biosynthetic gene clusters	2190:2220	Analyses of the draft genomes of the isolates and the C. acidiphila and C. rubra strains revealed the presence of many biosynthetic gene clusters with the potential to synthesize novel drug-like metabolites.
33360072	5	91	theme	phylogenomic	1260:1271	arg1	tree					1273:1276	the actinobacterial phylogenomic tree	1240:1276	the actinobacterial phylogenomic tree	1240:1276	The isolates and the C. acidiphila and C. rubra strains formed a well supported branch in the actinobacterial phylogenomic tree.
33360072	6	92	theme	rRNA	1356:1359	arg1	sequences					1366:1374	identical 16S rRNA gene sequences	1342:1374	identical 16S rRNA gene sequences	1342:1374	Isolates NF3 and NH11T belong to the same species as they have identical 16S rRNA gene sequences, share many chemotaxonomic, cultural and phenotypic features and show very high average nucleotide identity (ANI) and digital DNA:DNA relatedness (dDDH) similarities.
33360072	12	93	dep	In	2284:2285	arg1	vitro					2287:2291	vitro	2287:2291	vitro	2287:2291	In vitro screens showed that the isolates inhibited the growth of Gram-positive bacteria and wheat pathogens belonging to the genus Fusarium.
33360072	2	94	theme	pine	238:241	arg1	soil					250:253	a pine forest soil	236:253	a pine forest soil	236:253	Two actinobacteria, strains NF3 and NH11T, isolated from a pine forest soil, near Torun, Poland were examined for diverse chemotaxonomic and morphological properties that placed them in the genus Catenulispora.
33360072	6	95	theme	relatedness	1510:1520	arg1	similarities					1529:1540	digital DNA:DNA relatedness (dDDH) similarities	1494:1540	digital DNA:DNA relatedness (dDDH) similarities	1494:1540	Isolates NF3 and NH11T belong to the same species as they have identical 16S rRNA gene sequences, share many chemotaxonomic, cultural and phenotypic features and show very high average nucleotide identity (ANI) and digital DNA:DNA relatedness (dDDH) similarities.
33360072	1	96	theme	emended	135:141	arg1	description					143:153	emended description	135:153	emended description of Catenulispora rubra	135:176	nov., novel actinobacteria isolated from a pine forest soil in Poland and emended description of Catenulispora rubra.
33360072	3	97	theme	gene	799:802	arg1	tree					804:807	the Catenulispora 16S rRNA gene tree	772:807	the Catenulispora 16S rRNA gene tree	772:807	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	6	98	theme	identical	1342:1350	arg1	sequences					1366:1374	identical 16S rRNA gene sequences	1342:1374	identical 16S rRNA gene sequences	1342:1374	Isolates NF3 and NH11T belong to the same species as they have identical 16S rRNA gene sequences, share many chemotaxonomic, cultural and phenotypic features and show very high average nucleotide identity (ANI) and digital DNA:DNA relatedness (dDDH) similarities.
33360072	3	99	theme	sequence	902:909	arg1	similarities					911:922	sequence similarities	902:922	sequence similarities	902:922	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	2	100	attach	isolated	222:229	arg2	NH11T					215:219	NH11T	215:219	NH11T	215:219	Two actinobacteria, strains NF3 and NH11T, isolated from a pine forest soil, near Torun, Poland were examined for diverse chemotaxonomic and morphological properties that placed them in the genus Catenulispora.
33360072	2	100	attach	isolated	222:229	arg2	strains					199:205	strains NF3 and NH11T	199:219	strains NF3 and NH11T	199:219	Two actinobacteria, strains NF3 and NH11T, isolated from a pine forest soil, near Torun, Poland were examined for diverse chemotaxonomic and morphological properties that placed them in the genus Catenulispora.
33360072	2	100	attach	isolated	222:229	arg2	NF3					207:209	NF3	207:209	NF3	207:209	Two actinobacteria, strains NF3 and NH11T, isolated from a pine forest soil, near Torun, Poland were examined for diverse chemotaxonomic and morphological properties that placed them in the genus Catenulispora.
33360072	2	100	attach	isolated	222:229	arg2	actinobacteria					183:196	Two actinobacteria	179:196	Two actinobacteria	179:196	Two actinobacteria, strains NF3 and NH11T, isolated from a pine forest soil, near Torun, Poland were examined for diverse chemotaxonomic and morphological properties that placed them in the genus Catenulispora.
33360072	2	100	attach	isolated	222:229	arg1	soil					250:253	a pine forest soil	236:253	a pine forest soil	236:253	Two actinobacteria, strains NF3 and NH11T, isolated from a pine forest soil, near Torun, Poland were examined for diverse chemotaxonomic and morphological properties that placed them in the genus Catenulispora.
33360072	3	101	theme	DSM	848:850	arg1	44928T					852:857	Catenulispora acidiphila DSM 44928T	823:857	Catenulispora acidiphila DSM 44928T	823:857	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	7	102	theme	properties	1668:1677	arg1	combination					1623:1633	a combination	1621:1633	a combination of chemotaxonomic and phenotypic properties	1621:1677	They can be distinguished from their closest phylogenomic neighbours by using a combination of chemotaxonomic and phenotypic properties and by ANI and dDDH values well below the thresholds of these metrics used to assign closely related strains to different species.
33360072	11	103	theme	novel	2255:2259	arg1	metabolites					2271:2281	novel drug-like metabolites	2255:2281	novel drug-like metabolites	2255:2281	Analyses of the draft genomes of the isolates and the C. acidiphila and C. rubra strains revealed the presence of many biosynthetic gene clusters with the potential to synthesize novel drug-like metabolites.
33360072	12	104	theme	pathogens	2383:2391	arg1	growth					2340:2345	the growth	2336:2345	the growth of Gram-positive bacteria and wheat pathogens belonging to the genus Fusarium	2336:2423	In vitro screens showed that the isolates inhibited the growth of Gram-positive bacteria and wheat pathogens belonging to the genus Fusarium.
33360072	3	105	theme	Catenulispora	823:835	arg1	44928T					852:857	Catenulispora acidiphila DSM 44928T	823:857	Catenulispora acidiphila DSM 44928T	823:857	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	1	106	from	description	143:153	arg1	Poland					124:129	Poland	124:129	Poland	124:129	nov., novel actinobacteria isolated from a pine forest soil in Poland and emended description of Catenulispora rubra.
33360072	9	107	theme	=DSM	1960:1963	arg1	NH11T					1953:1957	NH11T	1953:1957	NH11T (=DSM 111109T =PCM 3046T)	1953:1983	nov., the type strain is NH11T (=DSM 111109T =PCM 3046T).
33360072	9	107	theme	=DSM	1960:1963	arg1	3046T					1978:1982	=DSM 111109T =PCM 3046T	1960:1982	=DSM 111109T =PCM 3046T	1960:1982	nov., the type strain is NH11T (=DSM 111109T =PCM 3046T).
33360072	2	108	theme	genus	369:373	arg1	Catenulispora					375:387	the genus Catenulispora	365:387	the genus Catenulispora	365:387	Two actinobacteria, strains NF3 and NH11T, isolated from a pine forest soil, near Torun, Poland were examined for diverse chemotaxonomic and morphological properties that placed them in the genus Catenulispora.
33360072	6	109	theme	cultural	1404:1411	arg1	features					1428:1435	many chemotaxonomic, cultural and phenotypic features	1383:1435	many chemotaxonomic, cultural and phenotypic features	1383:1435	Isolates NF3 and NH11T belong to the same species as they have identical 16S rRNA gene sequences, share many chemotaxonomic, cultural and phenotypic features and show very high average nucleotide identity (ANI) and digital DNA:DNA relatedness (dDDH) similarities.
33360072	9	110	theme	=PCM	1973:1976	arg1	NH11T					1953:1957	NH11T	1953:1957	NH11T (=DSM 111109T =PCM 3046T)	1953:1983	nov., the type strain is NH11T (=DSM 111109T =PCM 3046T).
33360072	9	110	theme	=PCM	1973:1976	arg1	3046T					1978:1982	=DSM 111109T =PCM 3046T	1960:1982	=DSM 111109T =PCM 3046T	1960:1982	nov., the type strain is NH11T (=DSM 111109T =PCM 3046T).
33360072	1	111	dep	Catenulispora	158:170	arg1	rubra					172:176	Catenulispora rubra	158:176	Catenulispora rubra	158:176	nov., novel actinobacteria isolated from a pine forest soil in Poland and emended description of Catenulispora rubra.
33360072	3	112	theme	branched	419:426	arg1	mycelium					435:442	an extensively branched stable mycelium	404:442	an extensively branched stable mycelium	404:442	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	0	113	theme	Catenulispora	32:44	arg1	sp					57:58	Catenulispora pinisilvae sp	32:58	Catenulispora pinisilvae sp	32:58	Genomic-based classification of Catenulispora pinisilvae sp.
33360072	4	114	dep	C.	1044:1045	arg1	rubra					1047:1051	rubra	1047:1051	rubra	1047:1051	The sizes of whole genome sequences generated for the isolates and the C. rubra strain ranged from 11.20 to 12.80 Mbp with corresponding in silico DNA G+C values of 69.9-70.0%.
33360072	1	115	theme	pine	104:107	arg1	soil					116:119	a pine forest soil	102:119	a pine forest soil in Poland	102:129	nov., novel actinobacteria isolated from a pine forest soil in Poland and emended description of Catenulispora rubra.
33360072	3	116	theme	99.0	952:955	arg1	%					956:956	98.8 and 99.0%	943:956	%	956:956	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	12	117	theme	Gram-positive	2350:2362	arg1	bacteria					2364:2371	Gram-positive bacteria	2350:2371	Gram-positive bacteria	2350:2371	In vitro screens showed that the isolates inhibited the growth of Gram-positive bacteria and wheat pathogens belonging to the genus Fusarium.
33360072	12	118	theme	wheat	2377:2381	arg1	pathogens					2383:2391	wheat pathogens	2377:2391	wheat pathogens	2377:2391	In vitro screens showed that the isolates inhibited the growth of Gram-positive bacteria and wheat pathogens belonging to the genus Fusarium.
33360072	0	119	theme	sp	57:58	arg1	classification					14:27	Genomic-based classification	0:27	Genomic-based classification of Catenulispora pinisilvae sp	0:58	Genomic-based classification of Catenulispora pinisilvae sp.
33360072	3	120	theme	98.8	943:946	arg1	%					956:956	98.8 and 99.0%	943:956	%	956:956	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	4	121	theme	genome	992:997	arg1	sequences					999:1007	whole genome sequences	986:1007	whole genome sequences generated for the isolates and the C. rubra strain	986:1058	The sizes of whole genome sequences generated for the isolates and the C. rubra strain ranged from 11.20 to 12.80 Mbp with corresponding in silico DNA G+C values of 69.9-70.0%.
33360072	6	122	theme	average	1456:1462	arg1	ANI					1485:1487	ANI	1485:1487	ANI	1485:1487	Isolates NF3 and NH11T belong to the same species as they have identical 16S rRNA gene sequences, share many chemotaxonomic, cultural and phenotypic features and show very high average nucleotide identity (ANI) and digital DNA:DNA relatedness (dDDH) similarities.
33360072	6	122	theme	average	1456:1462	arg1	identity					1475:1482	very high average nucleotide identity	1446:1482	very high average nucleotide identity (ANI)	1446:1488	Isolates NF3 and NH11T belong to the same species as they have identical 16S rRNA gene sequences, share many chemotaxonomic, cultural and phenotypic features and show very high average nucleotide identity (ANI) and digital DNA:DNA relatedness (dDDH) similarities.
33360072	2	123	dep	strains	199:205	arg1	strains					199:205	strains NF3 and NH11T	199:219	strains NF3 and NH11T	199:219	Two actinobacteria, strains NF3 and NH11T, isolated from a pine forest soil, near Torun, Poland were examined for diverse chemotaxonomic and morphological properties that placed them in the genus Catenulispora.
33360072	2	123	dep	strains	199:205	arg1	NH11T					215:219	NH11T	215:219	NH11T	215:219	Two actinobacteria, strains NF3 and NH11T, isolated from a pine forest soil, near Torun, Poland were examined for diverse chemotaxonomic and morphological properties that placed them in the genus Catenulispora.
33360072	2	123	dep	strains	199:205	arg1	NF3					207:209	NF3	207:209	NF3	207:209	Two actinobacteria, strains NF3 and NH11T, isolated from a pine forest soil, near Torun, Poland were examined for diverse chemotaxonomic and morphological properties that placed them in the genus Catenulispora.
33360072	2	124	theme	diverse	293:299	arg1	properties					334:343	diverse chemotaxonomic and morphological properties	293:343	diverse chemotaxonomic and morphological properties that placed them in the genus Catenulispora	293:387	Two actinobacteria, strains NF3 and NH11T, isolated from a pine forest soil, near Torun, Poland were examined for diverse chemotaxonomic and morphological properties that placed them in the genus Catenulispora.
33360072	7	125	theme	related	1772:1778	arg1	strains					1780:1786	closely related strains	1764:1786	closely related strains	1764:1786	They can be distinguished from their closest phylogenomic neighbours by using a combination of chemotaxonomic and phenotypic properties and by ANI and dDDH values well below the thresholds of these metrics used to assign closely related strains to different species.
33360072	11	126	theme	gene	2208:2211	arg1	clusters					2213:2220	many biosynthetic gene clusters	2190:2220	many biosynthetic gene clusters	2190:2220	Analyses of the draft genomes of the isolates and the C. acidiphila and C. rubra strains revealed the presence of many biosynthetic gene clusters with the potential to synthesize novel drug-like metabolites.
33360072	3	127	theme	major	712:716	arg1	acids					724:728	major fatty acids	712:728	major fatty acids	712:728	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33360072	3	128	with	anteiso-C17:0	695:707	arg1	units					640:644	nine isoprenoid units	624:644	nine isoprenoid units as the predominant isoprenologues	624:678	They produced an extensively branched stable mycelium, contained LL-diaminopimelic acid as the diamino acid of the peptidoglycan, arabinose as the diagnostic whole-organism sugar, tetra-, hexa- and octa-hydrogenated menaquinones with nine isoprenoid units as the predominant isoprenologues, iso-C16:0 and anteiso-C17:0 as major fatty acids, and formed a well supported clade within the Catenulispora 16S rRNA gene tree together with Catenulispora acidiphila DSM 44928T and Catenulispora rubra DSM 44948T sharing sequence similarities with the latter of 98.8 and 99.0%, respectively.
33041025	15	0	theme	meal	2294:2297	arg1	effects					2262:2268	no effects	2259:2268	no effects of substituting soybean meal by spirulina on total antioxidant capacity, α-tocopherol and total phenols in blood and milk	2259:2390	Furthermore, we observed no effects of substituting soybean meal by spirulina on total antioxidant capacity, α-tocopherol and total phenols in blood and milk.
33041025	14	1	from	groups	2226:2231	arg1	milk					2197:2200	milk	2197:2200	milk from the 2 experimental groups	2197:2231	No sensory difference was found between milk from the 2 experimental groups.
33041025	3	2	theme	individual	638:647	arg1	troughs					657:663	individual feeding troughs	638:663	individual feeding troughs	638:663	Twelve cows were allocated to 2 groups and fed hay-based diets complemented with sugar beet pulp and wheat flakes in individual feeding troughs.
33041025	11	3	theme	spirulina-fed	1629:1641	arg1	cows					1643:1646	the spirulina-fed cows	1625:1646	the spirulina-fed cows	1625:1646	However, the milk from the spirulina-fed cows had a higher content of β-carotene (0.207 vs. 0.135 μg/mL) and was more yellow (b* index: 14.9 vs. 13.8).
33041025	12	4	from	cows	1847:1850	arg1	fat					1820:1822	the milk fat	1811:1822	the milk fat from the spirulina-fed cows	1811:1850	Similar to the spirulina lipids but far less pronounced, the milk fat from the spirulina-fed cows had a higher proportion of γ-linolenic acid (0.057 vs. 0.038% of fatty acid methyl esters) compared with milk fat from soybean meal-fed cows.
33041025	1	5	theme	metabolizable	228:240	arg1	protein					242:248	metabolizable protein	228:248	metabolizable protein	228:248	The demand for protein sources alternative to soybean meal for supplementing forages low in metabolizable protein is large.
33041025	16	6	from	Effects	2393:2399	arg1	characteristics					2416:2430	rumen fluid characteristics	2404:2430	rumen fluid characteristics	2404:2430	Effects on rumen fluid characteristics were minor.
33041025	17	7	from	performance	2632:2642	arg1	term					2657:2660	the short term	2647:2660	the short term	2647:2660	In conclusion, spirulina seems to be a promising protein source for dairy cows with certain improvements in nutritionally favorable constituents in milk and without side-effects on animal performance in the short term.
33041025	3	8	from	pulp	613:616	arg1	troughs					657:663	individual feeding troughs	638:663	individual feeding troughs	638:663	Twelve cows were allocated to 2 groups and fed hay-based diets complemented with sugar beet pulp and wheat flakes in individual feeding troughs.
33041025	11	9	theme	*	1729:1729	arg1	index					1731:1735	b* index	1728:1735	b* index	1728:1735	However, the milk from the spirulina-fed cows had a higher content of β-carotene (0.207 vs. 0.135 μg/mL) and was more yellow (b* index: 14.9 vs. 13.8).
33041025	11	9	theme	*	1729:1729	arg1	milk					1615:1618	the milk	1611:1618	the milk from the spirulina-fed cows	1611:1646	However, the milk from the spirulina-fed cows had a higher content of β-carotene (0.207 vs. 0.135 μg/mL) and was more yellow (b* index: 14.9 vs. 13.8).
33041025	11	9	theme	*	1729:1729	arg1	yellow					1720:1725	yellow	1720:1725	yellow	1720:1725	However, the milk from the spirulina-fed cows had a higher content of β-carotene (0.207 vs. 0.135 μg/mL) and was more yellow (b* index: 14.9 vs. 13.8).
33041025	1	10	from	low	221:223	arg1	protein					242:248	metabolizable protein	228:248	metabolizable protein	228:248	The demand for protein sources alternative to soybean meal for supplementing forages low in metabolizable protein is large.
33041025	12	11	theme	soybean	1971:1977	arg1	cows					1988:1991	soybean meal-fed cows	1971:1991	soybean meal-fed cows	1971:1991	Similar to the spirulina lipids but far less pronounced, the milk fat from the spirulina-fed cows had a higher proportion of γ-linolenic acid (0.057 vs. 0.038% of fatty acid methyl esters) compared with milk fat from soybean meal-fed cows.
33041025	13	12	theme	other	2034:2038	arg1	isomers					2052:2058	other C18:1 trans isomers	2034:2058	other C18:1 trans isomers	2034:2058	Also trans-11 C18:1 (vaccenic acid) and other C18:1 trans isomers were elevated, but otherwise the fatty acid profile resembled that of cows fed the control diet.
33041025	8	13	theme	Milk	1116:1119	arg1	samples					1121:1127	Milk samples	1116:1127	Milk samples	1116:1127	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	5	14	theme	5	773:773	arg1	%					774:774	%	774:774	%	774:774	Diet of 1 group was supplemented with 5% spirulina; the second group was supplemented with 6% soybean meal (control).
33041025	8	15	theme	vitamins	1263:1270	arg1	contents					1202:1209	contents	1202:1209	contents	1202:1209	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	8	15	theme	vitamins	1263:1270	arg1	color					1191:1195	color	1191:1195	color	1191:1195	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	8	15	theme	vitamins	1263:1270	arg1	profile					1158:1164	fatty acid profile	1147:1164	fatty acid profile	1147:1164	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	8	15	theme	vitamins	1263:1270	arg1	properties					1179:1188	coagulation properties	1167:1188	coagulation properties	1167:1188	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	11	16	theme	higher	1654:1659	arg1	content					1661:1667	a higher content	1652:1667	a higher content of β-carotene (0.207 vs. 0.135 μg/mL)	1652:1705	However, the milk from the spirulina-fed cows had a higher content of β-carotene (0.207 vs. 0.135 μg/mL) and was more yellow (b* index: 14.9 vs. 13.8).
33041025	7	17	theme	blood	1011:1015	arg1	plasma					1017:1022	blood plasma	1011:1022	blood plasma	1011:1022	Feeds were analyzed for proximate contents, and blood plasma was analyzed for total antioxidant capacity and antioxidant contents (tocopherol, phenols).
33041025	17	18	from	constituents	2576:2587	arg1	milk					2592:2595	milk	2592:2595	milk	2592:2595	In conclusion, spirulina seems to be a promising protein source for dairy cows with certain improvements in nutritionally favorable constituents in milk and without side-effects on animal performance in the short term.
33041025	11	19	dep	yellow	1720:1725	arg1	index					1731:1735	b* index	1728:1735	b* index	1728:1735	However, the milk from the spirulina-fed cows had a higher content of β-carotene (0.207 vs. 0.135 μg/mL) and was more yellow (b* index: 14.9 vs. 13.8).
33041025	11	19	dep	yellow	1720:1725	arg1	milk					1615:1618	the milk	1611:1618	the milk from the spirulina-fed cows	1611:1646	However, the milk from the spirulina-fed cows had a higher content of β-carotene (0.207 vs. 0.135 μg/mL) and was more yellow (b* index: 14.9 vs. 13.8).
33041025	11	19	dep	yellow	1720:1725	arg1	yellow					1720:1725	yellow	1720:1725	yellow	1720:1725	However, the milk from the spirulina-fed cows had a higher content of β-carotene (0.207 vs. 0.135 μg/mL) and was more yellow (b* index: 14.9 vs. 13.8).
33041025	4	20	theme	N	670:670	arg1	content					672:678	The N content	666:678	The N content per kilogram of DM	666:697	The N content per kilogram of DM was equivalent between the 2 diets.
33041025	4	20	theme	N	670:670	arg1	equivalent					703:712	equivalent	703:712	equivalent	703:712	The N content per kilogram of DM was equivalent between the 2 diets.
33041025	0	21	theme	sensory	116:122	arg1	perception					124:133	sensory perception	116:133	sensory perception	116:133	Effects of the substitution of soybean meal by spirulina in a hay-based diet for dairy cows on milk composition and sensory perception.
33041025	13	22	theme	trans-11	1999:2006	arg1	acid					2024:2027	vaccenic acid	2015:2027	vaccenic acid	2015:2027	Also trans-11 C18:1 (vaccenic acid) and other C18:1 trans isomers were elevated, but otherwise the fatty acid profile resembled that of cows fed the control diet.
33041025	13	22	theme	trans-11	1999:2006	arg1	C18:1					2008:2012	trans-11 C18:1	1999:2012	trans-11 C18:1 (vaccenic acid)	1999:2028	Also trans-11 C18:1 (vaccenic acid) and other C18:1 trans isomers were elevated, but otherwise the fatty acid profile resembled that of cows fed the control diet.
33041025	5	23	theme	soybean	829:835	arg1	control					843:849	control	843:849	control	843:849	Diet of 1 group was supplemented with 5% spirulina; the second group was supplemented with 6% soybean meal (control).
33041025	5	23	theme	soybean	829:835	arg1	meal					837:840	6% soybean meal	826:840	6% soybean meal (control)	826:850	Diet of 1 group was supplemented with 5% spirulina; the second group was supplemented with 6% soybean meal (control).
33041025	16	24	theme	fluid	2410:2414	arg1	characteristics					2416:2430	rumen fluid characteristics	2404:2430	rumen fluid characteristics	2404:2430	Effects on rumen fluid characteristics were minor.
33041025	8	25	theme	acid	1153:1156	arg1	profile					1158:1164	fatty acid profile	1147:1164	fatty acid profile	1147:1164	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	17	26	theme	animal	2625:2630	arg1	performance					2632:2642	animal performance	2625:2642	animal performance in the short term	2625:2660	In conclusion, spirulina seems to be a promising protein source for dairy cows with certain improvements in nutritionally favorable constituents in milk and without side-effects on animal performance in the short term.
33041025	17	27	from	improvements	2536:2547	arg1	constituents					2576:2587	nutritionally favorable constituents	2552:2587	nutritionally favorable constituents in milk	2552:2595	In conclusion, spirulina seems to be a promising protein source for dairy cows with certain improvements in nutritionally favorable constituents in milk and without side-effects on animal performance in the short term.
33041025	12	28	dep	acid	1891:1894	arg1	esters					1935:1940	fatty acid methyl esters	1917:1940	fatty acid methyl esters	1917:1940	Similar to the spirulina lipids but far less pronounced, the milk fat from the spirulina-fed cows had a higher proportion of γ-linolenic acid (0.057 vs. 0.038% of fatty acid methyl esters) compared with milk fat from soybean meal-fed cows.
33041025	12	28	dep	acid	1891:1894	arg1	%					1912:1912	0.057 vs. 0.038%	1897:1912	0.057 vs. 0.038% of fatty acid methyl esters	1897:1940	Similar to the spirulina lipids but far less pronounced, the milk fat from the spirulina-fed cows had a higher proportion of γ-linolenic acid (0.057 vs. 0.038% of fatty acid methyl esters) compared with milk fat from soybean meal-fed cows.
33041025	11	29	dep	index	1731:1735	arg1	13.8					1747:1750	13.8	1747:1750	13.8	1747:1750	However, the milk from the spirulina-fed cows had a higher content of β-carotene (0.207 vs. 0.135 μg/mL) and was more yellow (b* index: 14.9 vs. 13.8).
33041025	11	29	dep	index	1731:1735	arg1	14.9					1738:1741	14.9	1738:1741	14.9	1738:1741	However, the milk from the spirulina-fed cows had a higher content of β-carotene (0.207 vs. 0.135 μg/mL) and was more yellow (b* index: 14.9 vs. 13.8).
33041025	2	30	theme	milk	455:458	arg1	antioxidants					460:471	milk antioxidants	455:471	milk antioxidants	455:471	The suitability of spirulina (Arthrospira platensis), a fast growing and resource-efficient blue-green microalga, as a source of metabolizable protein for dairy cows is known, but its effects on milk antioxidants and sensory properties were never investigated.
33041025	12	31	theme	meal-fed	1979:1986	arg1	cows					1988:1991	soybean meal-fed cows	1971:1991	soybean meal-fed cows	1971:1991	Similar to the spirulina lipids but far less pronounced, the milk fat from the spirulina-fed cows had a higher proportion of γ-linolenic acid (0.057 vs. 0.038% of fatty acid methyl esters) compared with milk fat from soybean meal-fed cows.
33041025	17	32	theme	dairy	2512:2516	arg1	cows					2518:2521	dairy cows	2512:2521	dairy cows with certain improvements in nutritionally favorable constituents in milk and without side-effects on animal performance in the short term	2512:2660	In conclusion, spirulina seems to be a promising protein source for dairy cows with certain improvements in nutritionally favorable constituents in milk and without side-effects on animal performance in the short term.
33041025	10	33	theme	control	1587:1593	arg1	group					1595:1599	the control group	1583:1599	the control group	1583:1599	The substitution of soybean meal by spirulina in the diet did not affect feed intake, milk yield, milk fat, protein, or lactose contents compared with the control group.
33041025	15	34	from	α-tocopherol	2343:2354	arg1	milk					2387:2390	milk	2387:2390	milk	2387:2390	Furthermore, we observed no effects of substituting soybean meal by spirulina on total antioxidant capacity, α-tocopherol and total phenols in blood and milk.
33041025	15	34	from	α-tocopherol	2343:2354	arg1	blood					2377:2381	blood	2377:2381	blood	2377:2381	Furthermore, we observed no effects of substituting soybean meal by spirulina on total antioxidant capacity, α-tocopherol and total phenols in blood and milk.
33041025	7	35	theme	antioxidant	1072:1082	arg1	contents					1084:1091	antioxidant contents	1072:1091	antioxidant contents (tocopherol, phenols)	1072:1113	Feeds were analyzed for proximate contents, and blood plasma was analyzed for total antioxidant capacity and antioxidant contents (tocopherol, phenols).
33041025	5	36	theme	6	826:826	arg1	%					827:827	%	827:827	%	827:827	Diet of 1 group was supplemented with 5% spirulina; the second group was supplemented with 6% soybean meal (control).
33041025	0	37	from	Effects	0:6	arg1	diet					72:75	a hay-based diet	60:75	a hay-based diet for dairy cows on milk composition and sensory perception	60:133	Effects of the substitution of soybean meal by spirulina in a hay-based diet for dairy cows on milk composition and sensory perception.
33041025	2	38	dep	growing	321:327	arg1	fast					316:319	fast	316:319	fast	316:319	The suitability of spirulina (Arthrospira platensis), a fast growing and resource-efficient blue-green microalga, as a source of metabolizable protein for dairy cows is known, but its effects on milk antioxidants and sensory properties were never investigated.
33041025	4	39	theme	DM	696:697	arg1	kilogram					684:691	kilogram	684:691	kilogram of DM	684:697	The N content per kilogram of DM was equivalent between the 2 diets.
33041025	17	40	theme	short	2651:2655	arg1	term					2657:2660	the short term	2647:2660	the short term	2647:2660	In conclusion, spirulina seems to be a promising protein source for dairy cows with certain improvements in nutritionally favorable constituents in milk and without side-effects on animal performance in the short term.
33041025	9	41	theme	sensory	1352:1358	arg1	panel					1360:1364	a trained sensory panel	1342:1364	a trained sensory panel	1342:1364	Triangle tests were performed by a trained sensory panel on 6 homogenized and pasteurized bulk milk samples per treatment.
33041025	12	42	theme	acid	1923:1926	arg1	esters					1935:1940	fatty acid methyl esters	1917:1940	fatty acid methyl esters	1917:1940	Similar to the spirulina lipids but far less pronounced, the milk fat from the spirulina-fed cows had a higher proportion of γ-linolenic acid (0.057 vs. 0.038% of fatty acid methyl esters) compared with milk fat from soybean meal-fed cows.
33041025	12	43	theme	fatty	1917:1921	arg1	esters					1935:1940	fatty acid methyl esters	1917:1940	fatty acid methyl esters	1917:1940	Similar to the spirulina lipids but far less pronounced, the milk fat from the spirulina-fed cows had a higher proportion of γ-linolenic acid (0.057 vs. 0.038% of fatty acid methyl esters) compared with milk fat from soybean meal-fed cows.
33041025	7	44	theme	total	1041:1045	arg1	capacity					1059:1066	total antioxidant capacity	1041:1066	total antioxidant capacity	1041:1066	Feeds were analyzed for proximate contents, and blood plasma was analyzed for total antioxidant capacity and antioxidant contents (tocopherol, phenols).
33041025	12	45	from	cows	1988:1991	arg1	fat					1962:1964	milk fat	1957:1964	milk fat from soybean meal-fed cows	1957:1991	Similar to the spirulina lipids but far less pronounced, the milk fat from the spirulina-fed cows had a higher proportion of γ-linolenic acid (0.057 vs. 0.038% of fatty acid methyl esters) compared with milk fat from soybean meal-fed cows.
33041025	13	46	theme	fatty	2093:2097	arg1	profile					2104:2110	otherwise the fatty acid profile	2079:2110	otherwise the fatty acid profile	2079:2110	Also trans-11 C18:1 (vaccenic acid) and other C18:1 trans isomers were elevated, but otherwise the fatty acid profile resembled that of cows fed the control diet.
33041025	12	47	contain	had	1852:1854	arg1	fat					1820:1822	the milk fat	1811:1822	the milk fat from the spirulina-fed cows	1811:1850	Similar to the spirulina lipids but far less pronounced, the milk fat from the spirulina-fed cows had a higher proportion of γ-linolenic acid (0.057 vs. 0.038% of fatty acid methyl esters) compared with milk fat from soybean meal-fed cows.
33041025	12	47	contain	had	1852:1854	arg2	proportion					1865:1874	a higher proportion	1856:1874	a higher proportion of γ-linolenic acid (0.057 vs. 0.038% of fatty acid methyl esters)	1856:1941	Similar to the spirulina lipids but far less pronounced, the milk fat from the spirulina-fed cows had a higher proportion of γ-linolenic acid (0.057 vs. 0.038% of fatty acid methyl esters) compared with milk fat from soybean meal-fed cows.
33041025	12	48	theme	esters	1935:1940	arg1	esters					1935:1940	fatty acid methyl esters	1917:1940	fatty acid methyl esters	1917:1940	Similar to the spirulina lipids but far less pronounced, the milk fat from the spirulina-fed cows had a higher proportion of γ-linolenic acid (0.057 vs. 0.038% of fatty acid methyl esters) compared with milk fat from soybean meal-fed cows.
33041025	12	48	theme	esters	1935:1940	arg1	%					1912:1912	0.057 vs. 0.038%	1897:1912	0.057 vs. 0.038% of fatty acid methyl esters	1897:1940	Similar to the spirulina lipids but far less pronounced, the milk fat from the spirulina-fed cows had a higher proportion of γ-linolenic acid (0.057 vs. 0.038% of fatty acid methyl esters) compared with milk fat from soybean meal-fed cows.
33041025	10	49	theme	soybean	1452:1458	arg1	meal					1460:1463	soybean meal	1452:1463	soybean meal	1452:1463	The substitution of soybean meal by spirulina in the diet did not affect feed intake, milk yield, milk fat, protein, or lactose contents compared with the control group.
33041025	5	50	theme	group	745:749	arg1	Diet					735:738	Diet	735:738	Diet of 1 group	735:749	Diet of 1 group was supplemented with 5% spirulina; the second group was supplemented with 6% soybean meal (control).
33041025	8	51	dep	β-carotene	1296:1305	arg1	e.g.					1290:1293	e.g.	1290:1293	e.g.	1290:1293	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	15	52	from	capacity	2333:2340	arg1	milk					2387:2390	milk	2387:2390	milk	2387:2390	Furthermore, we observed no effects of substituting soybean meal by spirulina on total antioxidant capacity, α-tocopherol and total phenols in blood and milk.
33041025	15	52	from	capacity	2333:2340	arg1	blood					2377:2381	blood	2377:2381	blood	2377:2381	Furthermore, we observed no effects of substituting soybean meal by spirulina on total antioxidant capacity, α-tocopherol and total phenols in blood and milk.
33041025	9	53	theme	homogenized	1371:1381	arg1	samples					1409:1415	6 homogenized and pasteurized bulk milk samples	1369:1415	6 homogenized and pasteurized bulk milk samples per treatment	1369:1429	Triangle tests were performed by a trained sensory panel on 6 homogenized and pasteurized bulk milk samples per treatment.
33041025	2	54	theme	metabolizable	389:401	arg1	protein					403:409	metabolizable protein	389:409	metabolizable protein	389:409	The suitability of spirulina (Arthrospira platensis), a fast growing and resource-efficient blue-green microalga, as a source of metabolizable protein for dairy cows is known, but its effects on milk antioxidants and sensory properties were never investigated.
33041025	11	55	theme	b	1728:1728	arg1	index					1731:1735	b* index	1728:1735	b* index	1728:1735	However, the milk from the spirulina-fed cows had a higher content of β-carotene (0.207 vs. 0.135 μg/mL) and was more yellow (b* index: 14.9 vs. 13.8).
33041025	11	55	theme	b	1728:1728	arg1	milk					1615:1618	the milk	1611:1618	the milk from the spirulina-fed cows	1611:1646	However, the milk from the spirulina-fed cows had a higher content of β-carotene (0.207 vs. 0.135 μg/mL) and was more yellow (b* index: 14.9 vs. 13.8).
33041025	11	55	theme	b	1728:1728	arg1	yellow					1720:1725	yellow	1720:1725	yellow	1720:1725	However, the milk from the spirulina-fed cows had a higher content of β-carotene (0.207 vs. 0.135 μg/mL) and was more yellow (b* index: 14.9 vs. 13.8).
33041025	9	56	theme	pasteurized	1387:1397	arg1	samples					1409:1415	6 homogenized and pasteurized bulk milk samples	1369:1415	6 homogenized and pasteurized bulk milk samples per treatment	1369:1429	Triangle tests were performed by a trained sensory panel on 6 homogenized and pasteurized bulk milk samples per treatment.
33041025	15	57	theme	substituting	2273:2284	arg1	meal					2294:2297	substituting soybean meal	2273:2297	substituting soybean meal by spirulina	2273:2310	Furthermore, we observed no effects of substituting soybean meal by spirulina on total antioxidant capacity, α-tocopherol and total phenols in blood and milk.
33041025	7	58	dep	contents	1084:1091	arg1	phenols					1106:1112	phenols	1106:1112	phenols	1106:1112	Feeds were analyzed for proximate contents, and blood plasma was analyzed for total antioxidant capacity and antioxidant contents (tocopherol, phenols).
33041025	7	58	dep	contents	1084:1091	arg1	tocopherol					1094:1103	tocopherol	1094:1103	tocopherol	1094:1103	Feeds were analyzed for proximate contents, and blood plasma was analyzed for total antioxidant capacity and antioxidant contents (tocopherol, phenols).
33041025	11	59	theme	β-carotene	1672:1681	arg1	content					1661:1667	a higher content	1652:1667	a higher content of β-carotene (0.207 vs. 0.135 μg/mL)	1652:1705	However, the milk from the spirulina-fed cows had a higher content of β-carotene (0.207 vs. 0.135 μg/mL) and was more yellow (b* index: 14.9 vs. 13.8).
33041025	15	60	theme	total	2360:2364	arg1	phenols					2366:2372	total phenols	2360:2372	total phenols	2360:2372	Furthermore, we observed no effects of substituting soybean meal by spirulina on total antioxidant capacity, α-tocopherol and total phenols in blood and milk.
33041025	9	61	theme	milk	1404:1407	arg1	samples					1409:1415	6 homogenized and pasteurized bulk milk samples	1369:1415	6 homogenized and pasteurized bulk milk samples per treatment	1369:1429	Triangle tests were performed by a trained sensory panel on 6 homogenized and pasteurized bulk milk samples per treatment.
33041025	3	62	theme	sugar	602:606	arg1	pulp					613:616	sugar beet pulp and wheat flakes	602:633	pulp	613:616	Twelve cows were allocated to 2 groups and fed hay-based diets complemented with sugar beet pulp and wheat flakes in individual feeding troughs.
33041025	12	63	theme	γ-linolenic	1879:1889	arg1	acid					1891:1894	γ-linolenic acid	1879:1894	γ-linolenic acid (0.057 vs. 0.038% of fatty acid methyl esters)	1879:1941	Similar to the spirulina lipids but far less pronounced, the milk fat from the spirulina-fed cows had a higher proportion of γ-linolenic acid (0.057 vs. 0.038% of fatty acid methyl esters) compared with milk fat from soybean meal-fed cows.
33041025	10	64	theme	feed	1505:1508	arg1	intake					1510:1515	feed intake	1505:1515	feed intake	1505:1515	The substitution of soybean meal by spirulina in the diet did not affect feed intake, milk yield, milk fat, protein, or lactose contents compared with the control group.
33041025	0	65	theme	meal	39:42	arg1	substitution					15:26	the substitution	11:26	the substitution of soybean meal by spirulina	11:55	Effects of the substitution of soybean meal by spirulina in a hay-based diet for dairy cows on milk composition and sensory perception.
33041025	13	66	theme	trans	2046:2050	arg1	isomers					2052:2058	other C18:1 trans isomers	2034:2058	other C18:1 trans isomers	2034:2058	Also trans-11 C18:1 (vaccenic acid) and other C18:1 trans isomers were elevated, but otherwise the fatty acid profile resembled that of cows fed the control diet.
33041025	3	67	theme	hay-based	568:576	arg1	diets					578:582	fed hay-based diets	564:582	fed hay-based diets	564:582	Twelve cows were allocated to 2 groups and fed hay-based diets complemented with sugar beet pulp and wheat flakes in individual feeding troughs.
33041025	8	68	theme	phenols	1243:1249	arg1	contents					1202:1209	contents	1202:1209	contents	1202:1209	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	8	68	theme	phenols	1243:1249	arg1	color					1191:1195	color	1191:1195	color	1191:1195	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	8	68	theme	phenols	1243:1249	arg1	profile					1158:1164	fatty acid profile	1147:1164	fatty acid profile	1147:1164	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	8	68	theme	phenols	1243:1249	arg1	properties					1179:1188	coagulation properties	1167:1188	coagulation properties	1167:1188	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	12	69	theme	higher	1858:1863	arg1	proportion					1865:1874	a higher proportion	1856:1874	a higher proportion of γ-linolenic acid (0.057 vs. 0.038% of fatty acid methyl esters)	1856:1941	Similar to the spirulina lipids but far less pronounced, the milk fat from the spirulina-fed cows had a higher proportion of γ-linolenic acid (0.057 vs. 0.038% of fatty acid methyl esters) compared with milk fat from soybean meal-fed cows.
33041025	17	70	theme	promising	2483:2491	arg1	spirulina					2459:2467	spirulina	2459:2467	spirulina	2459:2467	In conclusion, spirulina seems to be a promising protein source for dairy cows with certain improvements in nutritionally favorable constituents in milk and without side-effects on animal performance in the short term.
33041025	17	70	theme	promising	2483:2491	arg1	source					2501:2506	a promising protein source	2481:2506	a promising protein source for dairy cows with certain improvements in nutritionally favorable constituents in milk and without side-effects on animal performance in the short term	2481:2660	In conclusion, spirulina seems to be a promising protein source for dairy cows with certain improvements in nutritionally favorable constituents in milk and without side-effects on animal performance in the short term.
33041025	13	71	theme	vaccenic	2015:2022	arg1	acid					2024:2027	vaccenic acid	2015:2027	vaccenic acid	2015:2027	Also trans-11 C18:1 (vaccenic acid) and other C18:1 trans isomers were elevated, but otherwise the fatty acid profile resembled that of cows fed the control diet.
33041025	13	71	theme	vaccenic	2015:2022	arg1	C18:1					2008:2012	trans-11 C18:1	1999:2012	trans-11 C18:1 (vaccenic acid)	1999:2028	Also trans-11 C18:1 (vaccenic acid) and other C18:1 trans isomers were elevated, but otherwise the fatty acid profile resembled that of cows fed the control diet.
33041025	3	72	theme	wheat	622:626	arg1	flakes					628:633	sugar beet pulp and wheat flakes	602:633	flakes	628:633	Twelve cows were allocated to 2 groups and fed hay-based diets complemented with sugar beet pulp and wheat flakes in individual feeding troughs.
33041025	8	73	theme	lipophilic	1252:1261	arg1	vitamins					1263:1270	lipophilic vitamins	1252:1270	lipophilic vitamins	1252:1270	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	2	74	theme	resource-efficient	333:350	arg1	suitability					264:274	The suitability	260:274	The suitability of spirulina (Arthrospira platensis)	260:311	The suitability of spirulina (Arthrospira platensis), a fast growing and resource-efficient blue-green microalga, as a source of metabolizable protein for dairy cows is known, but its effects on milk antioxidants and sensory properties were never investigated.
33041025	2	74	theme	resource-efficient	333:350	arg1	microalga					363:371	a fast growing and resource-efficient blue-green microalga	314:371	a fast growing and resource-efficient blue-green microalga	314:371	The suitability of spirulina (Arthrospira platensis), a fast growing and resource-efficient blue-green microalga, as a source of metabolizable protein for dairy cows is known, but its effects on milk antioxidants and sensory properties were never investigated.
33041025	3	75	from	flakes	628:633	arg1	troughs					657:663	individual feeding troughs	638:663	individual feeding troughs	638:663	Twelve cows were allocated to 2 groups and fed hay-based diets complemented with sugar beet pulp and wheat flakes in individual feeding troughs.
33041025	2	76	theme	growing	321:327	arg1	suitability					264:274	The suitability	260:274	The suitability of spirulina (Arthrospira platensis)	260:311	The suitability of spirulina (Arthrospira platensis), a fast growing and resource-efficient blue-green microalga, as a source of metabolizable protein for dairy cows is known, but its effects on milk antioxidants and sensory properties were never investigated.
33041025	2	76	theme	growing	321:327	arg1	microalga					363:371	a fast growing and resource-efficient blue-green microalga	314:371	a fast growing and resource-efficient blue-green microalga	314:371	The suitability of spirulina (Arthrospira platensis), a fast growing and resource-efficient blue-green microalga, as a source of metabolizable protein for dairy cows is known, but its effects on milk antioxidants and sensory properties were never investigated.
33041025	17	77	with	cows	2518:2521	arg1	improvements					2536:2547	certain improvements	2528:2547	certain improvements in nutritionally favorable constituents in milk	2528:2595	In conclusion, spirulina seems to be a promising protein source for dairy cows with certain improvements in nutritionally favorable constituents in milk and without side-effects on animal performance in the short term.
33041025	3	78	theme	feeding	649:655	arg1	troughs					657:663	individual feeding troughs	638:663	individual feeding troughs	638:663	Twelve cows were allocated to 2 groups and fed hay-based diets complemented with sugar beet pulp and wheat flakes in individual feeding troughs.
33041025	2	79	theme	Arthrospira	290:300	arg1	platensis					302:310	spirulina (Arthrospira platensis)	279:311	spirulina (Arthrospira platensis)	279:311	The suitability of spirulina (Arthrospira platensis), a fast growing and resource-efficient blue-green microalga, as a source of metabolizable protein for dairy cows is known, but its effects on milk antioxidants and sensory properties were never investigated.
33041025	13	80	theme	control	2143:2149	arg1	diet					2151:2154	the control diet	2139:2154	the control diet	2139:2154	Also trans-11 C18:1 (vaccenic acid) and other C18:1 trans isomers were elevated, but otherwise the fatty acid profile resembled that of cows fed the control diet.
33041025	10	81	theme	milk	1530:1533	arg1	fat					1535:1537	milk fat	1530:1537	milk fat	1530:1537	The substitution of soybean meal by spirulina in the diet did not affect feed intake, milk yield, milk fat, protein, or lactose contents compared with the control group.
33041025	1	82	theme	protein	151:157	arg1	sources					159:165	protein sources	151:165	protein sources alternative to soybean meal	151:193	The demand for protein sources alternative to soybean meal for supplementing forages low in metabolizable protein is large.
33041025	10	83	from	substitution	1436:1447	arg1	diet					1485:1488	the diet	1481:1488	the diet	1481:1488	The substitution of soybean meal by spirulina in the diet did not affect feed intake, milk yield, milk fat, protein, or lactose contents compared with the control group.
33041025	12	84	theme	milk	1815:1818	arg1	fat					1820:1822	the milk fat	1811:1822	the milk fat from the spirulina-fed cows	1811:1850	Similar to the spirulina lipids but far less pronounced, the milk fat from the spirulina-fed cows had a higher proportion of γ-linolenic acid (0.057 vs. 0.038% of fatty acid methyl esters) compared with milk fat from soybean meal-fed cows.
33041025	15	85	theme	total	2315:2319	arg1	capacity					2333:2340	total antioxidant capacity	2315:2340	total antioxidant capacity	2315:2340	Furthermore, we observed no effects of substituting soybean meal by spirulina on total antioxidant capacity, α-tocopherol and total phenols in blood and milk.
33041025	10	86	theme	lactose	1552:1558	arg1	contents					1560:1567	lactose contents	1552:1567	lactose contents	1552:1567	The substitution of soybean meal by spirulina in the diet did not affect feed intake, milk yield, milk fat, protein, or lactose contents compared with the control group.
33041025	5	87	theme	second	791:796	arg1	group					798:802	the second group	787:802	the second group	787:802	Diet of 1 group was supplemented with 5% spirulina; the second group was supplemented with 6% soybean meal (control).
33041025	0	88	theme	milk	95:98	arg1	composition					100:110	milk composition	95:110	milk composition	95:110	Effects of the substitution of soybean meal by spirulina in a hay-based diet for dairy cows on milk composition and sensory perception.
33041025	2	89	theme	spirulina	279:287	arg1	platensis					302:310	spirulina (Arthrospira platensis)	279:311	spirulina (Arthrospira platensis)	279:311	The suitability of spirulina (Arthrospira platensis), a fast growing and resource-efficient blue-green microalga, as a source of metabolizable protein for dairy cows is known, but its effects on milk antioxidants and sensory properties were never investigated.
33041025	17	90	from	side-effects	2609:2620	arg1	performance					2632:2642	animal performance	2625:2642	animal performance in the short term	2625:2660	In conclusion, spirulina seems to be a promising protein source for dairy cows with certain improvements in nutritionally favorable constituents in milk and without side-effects on animal performance in the short term.
33041025	1	91	theme	alternative	167:177	arg1	sources					159:165	protein sources	151:165	protein sources alternative to soybean meal	151:193	The demand for protein sources alternative to soybean meal for supplementing forages low in metabolizable protein is large.
33041025	6	92	theme	adaptation	862:871	arg1	period					873:878	an adaptation period	859:878	an adaptation period of 15 d	859:886	After an adaptation period of 15 d, data were collected, and feed, milk, blood, and rumen fluid were sampled.
33041025	2	93	from	effects	444:450	arg1	antioxidants					460:471	milk antioxidants	455:471	milk antioxidants	455:471	The suitability of spirulina (Arthrospira platensis), a fast growing and resource-efficient blue-green microalga, as a source of metabolizable protein for dairy cows is known, but its effects on milk antioxidants and sensory properties were never investigated.
33041025	2	93	from	effects	444:450	arg1	properties					485:494	sensory properties	477:494	sensory properties	477:494	The suitability of spirulina (Arthrospira platensis), a fast growing and resource-efficient blue-green microalga, as a source of metabolizable protein for dairy cows is known, but its effects on milk antioxidants and sensory properties were never investigated.
33041025	5	94	theme	%	774:774	arg1	spirulina					776:784	5% spirulina	773:784	5% spirulina	773:784	Diet of 1 group was supplemented with 5% spirulina; the second group was supplemented with 6% soybean meal (control).
33041025	8	95	theme	provitamins	1277:1287	arg1	contents					1202:1209	contents	1202:1209	contents	1202:1209	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	8	95	theme	provitamins	1277:1287	arg1	color					1191:1195	color	1191:1195	color	1191:1195	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	8	95	theme	provitamins	1277:1287	arg1	profile					1158:1164	fatty acid profile	1147:1164	fatty acid profile	1147:1164	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	8	95	theme	provitamins	1277:1287	arg1	properties					1179:1188	coagulation properties	1167:1188	coagulation properties	1167:1188	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	13	96	theme	acid	2099:2102	arg1	profile					2104:2110	otherwise the fatty acid profile	2079:2110	otherwise the fatty acid profile	2079:2110	Also trans-11 C18:1 (vaccenic acid) and other C18:1 trans isomers were elevated, but otherwise the fatty acid profile resembled that of cows fed the control diet.
33041025	5	97	theme	%	827:827	arg1	control					843:849	control	843:849	control	843:849	Diet of 1 group was supplemented with 5% spirulina; the second group was supplemented with 6% soybean meal (control).
33041025	5	97	theme	%	827:827	arg1	meal					837:840	6% soybean meal	826:840	6% soybean meal (control)	826:850	Diet of 1 group was supplemented with 5% spirulina; the second group was supplemented with 6% soybean meal (control).
33041025	12	98	theme	milk	1957:1960	arg1	fat					1962:1964	milk fat	1957:1964	milk fat from soybean meal-fed cows	1957:1991	Similar to the spirulina lipids but far less pronounced, the milk fat from the spirulina-fed cows had a higher proportion of γ-linolenic acid (0.057 vs. 0.038% of fatty acid methyl esters) compared with milk fat from soybean meal-fed cows.
33041025	1	99	from	protein	242:248	arg1	low					221:223	low	221:223	low	221:223	The demand for protein sources alternative to soybean meal for supplementing forages low in metabolizable protein is large.
33041025	0	100	from	cows	87:90	arg1	composition					100:110	milk composition	95:110	milk composition	95:110	Effects of the substitution of soybean meal by spirulina in a hay-based diet for dairy cows on milk composition and sensory perception.
33041025	0	100	from	cows	87:90	arg1	perception					124:133	sensory perception	116:133	sensory perception	116:133	Effects of the substitution of soybean meal by spirulina in a hay-based diet for dairy cows on milk composition and sensory perception.
33041025	12	101	theme	spirulina	1769:1777	arg1	lipids					1779:1784	the spirulina lipids	1765:1784	the spirulina lipids	1765:1784	Similar to the spirulina lipids but far less pronounced, the milk fat from the spirulina-fed cows had a higher proportion of γ-linolenic acid (0.057 vs. 0.038% of fatty acid methyl esters) compared with milk fat from soybean meal-fed cows.
33041025	9	102	theme	Triangle	1309:1316	arg1	tests					1318:1322	Triangle tests	1309:1322	Triangle tests	1309:1322	Triangle tests were performed by a trained sensory panel on 6 homogenized and pasteurized bulk milk samples per treatment.
33041025	16	103	theme	rumen	2404:2408	arg1	characteristics					2416:2430	rumen fluid characteristics	2404:2430	rumen fluid characteristics	2404:2430	Effects on rumen fluid characteristics were minor.
33041025	8	104	theme	fatty	1147:1151	arg1	profile					1158:1164	fatty acid profile	1147:1164	fatty acid profile	1147:1164	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	15	105	theme	antioxidant	2321:2331	arg1	capacity					2333:2340	total antioxidant capacity	2315:2340	total antioxidant capacity	2315:2340	Furthermore, we observed no effects of substituting soybean meal by spirulina on total antioxidant capacity, α-tocopherol and total phenols in blood and milk.
33041025	3	106	theme	fed	564:566	arg1	diets					578:582	fed hay-based diets	564:582	fed hay-based diets	564:582	Twelve cows were allocated to 2 groups and fed hay-based diets complemented with sugar beet pulp and wheat flakes in individual feeding troughs.
33041025	14	107	theme	experimental	2213:2224	arg1	groups					2226:2231	the 2 experimental groups	2207:2231	the 2 experimental groups	2207:2231	No sensory difference was found between milk from the 2 experimental groups.
33041025	0	108	theme	hay-based	62:70	arg1	diet					72:75	a hay-based diet	60:75	a hay-based diet for dairy cows on milk composition and sensory perception	60:133	Effects of the substitution of soybean meal by spirulina in a hay-based diet for dairy cows on milk composition and sensory perception.
33041025	15	109	from	phenols	2366:2372	arg1	milk					2387:2390	milk	2387:2390	milk	2387:2390	Furthermore, we observed no effects of substituting soybean meal by spirulina on total antioxidant capacity, α-tocopherol and total phenols in blood and milk.
33041025	15	109	from	phenols	2366:2372	arg1	blood					2377:2381	blood	2377:2381	blood	2377:2381	Furthermore, we observed no effects of substituting soybean meal by spirulina on total antioxidant capacity, α-tocopherol and total phenols in blood and milk.
33041025	8	110	theme	coagulation	1167:1177	arg1	properties					1179:1188	coagulation properties	1167:1188	coagulation properties	1167:1188	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	7	111	theme	antioxidant	1047:1057	arg1	capacity					1059:1066	total antioxidant capacity	1041:1066	total antioxidant capacity	1041:1066	Feeds were analyzed for proximate contents, and blood plasma was analyzed for total antioxidant capacity and antioxidant contents (tocopherol, phenols).
33041025	12	112	theme	spirulina-fed	1833:1845	arg1	cows					1847:1850	the spirulina-fed cows	1829:1850	the spirulina-fed cows	1829:1850	Similar to the spirulina lipids but far less pronounced, the milk fat from the spirulina-fed cows had a higher proportion of γ-linolenic acid (0.057 vs. 0.038% of fatty acid methyl esters) compared with milk fat from soybean meal-fed cows.
33041025	9	113	theme	trained	1344:1350	arg1	panel					1360:1364	a trained sensory panel	1342:1364	a trained sensory panel	1342:1364	Triangle tests were performed by a trained sensory panel on 6 homogenized and pasteurized bulk milk samples per treatment.
33041025	6	114	theme	d	886:886	arg1	period					873:878	an adaptation period	859:878	an adaptation period of 15 d	859:886	After an adaptation period of 15 d, data were collected, and feed, milk, blood, and rumen fluid were sampled.
33041025	2	115	theme	sensory	477:483	arg1	properties					485:494	sensory properties	477:494	sensory properties	477:494	The suitability of spirulina (Arthrospira platensis), a fast growing and resource-efficient blue-green microalga, as a source of metabolizable protein for dairy cows is known, but its effects on milk antioxidants and sensory properties were never investigated.
33041025	12	116	theme	methyl	1928:1933	arg1	esters					1935:1940	fatty acid methyl esters	1917:1940	fatty acid methyl esters	1917:1940	Similar to the spirulina lipids but far less pronounced, the milk fat from the spirulina-fed cows had a higher proportion of γ-linolenic acid (0.057 vs. 0.038% of fatty acid methyl esters) compared with milk fat from soybean meal-fed cows.
33041025	11	117	from	cows	1643:1646	arg1	milk					1615:1618	the milk	1611:1618	the milk from the spirulina-fed cows	1611:1646	However, the milk from the spirulina-fed cows had a higher content of β-carotene (0.207 vs. 0.135 μg/mL) and was more yellow (b* index: 14.9 vs. 13.8).
33041025	11	117	from	cows	1643:1646	arg1	index					1731:1735	b* index	1728:1735	b* index	1728:1735	However, the milk from the spirulina-fed cows had a higher content of β-carotene (0.207 vs. 0.135 μg/mL) and was more yellow (b* index: 14.9 vs. 13.8).
33041025	11	117	from	cows	1643:1646	arg1	yellow					1720:1725	yellow	1720:1725	yellow	1720:1725	However, the milk from the spirulina-fed cows had a higher content of β-carotene (0.207 vs. 0.135 μg/mL) and was more yellow (b* index: 14.9 vs. 13.8).
33041025	17	118	theme	protein	2493:2499	arg1	spirulina					2459:2467	spirulina	2459:2467	spirulina	2459:2467	In conclusion, spirulina seems to be a promising protein source for dairy cows with certain improvements in nutritionally favorable constituents in milk and without side-effects on animal performance in the short term.
33041025	17	118	theme	protein	2493:2499	arg1	source					2501:2506	a promising protein source	2481:2506	a promising protein source for dairy cows with certain improvements in nutritionally favorable constituents in milk and without side-effects on animal performance in the short term	2481:2660	In conclusion, spirulina seems to be a promising protein source for dairy cows with certain improvements in nutritionally favorable constituents in milk and without side-effects on animal performance in the short term.
33041025	15	119	theme	soybean	2286:2292	arg1	meal					2294:2297	substituting soybean meal	2273:2297	substituting soybean meal by spirulina	2273:2310	Furthermore, we observed no effects of substituting soybean meal by spirulina on total antioxidant capacity, α-tocopherol and total phenols in blood and milk.
33041025	6	120	theme	rumen	937:941	arg1	fluid					943:947	rumen fluid	937:947	rumen fluid	937:947	After an adaptation period of 15 d, data were collected, and feed, milk, blood, and rumen fluid were sampled.
33041025	10	121	theme	meal	1460:1463	arg1	substitution					1436:1447	The substitution	1432:1447	The substitution of soybean meal by spirulina in the diet	1432:1488	The substitution of soybean meal by spirulina in the diet did not affect feed intake, milk yield, milk fat, protein, or lactose contents compared with the control group.
33041025	15	122	from	effects	2262:2268	arg1	α-tocopherol					2343:2354	α-tocopherol	2343:2354	α-tocopherol	2343:2354	Furthermore, we observed no effects of substituting soybean meal by spirulina on total antioxidant capacity, α-tocopherol and total phenols in blood and milk.
33041025	15	122	from	effects	2262:2268	arg1	phenols					2366:2372	total phenols	2360:2372	total phenols	2360:2372	Furthermore, we observed no effects of substituting soybean meal by spirulina on total antioxidant capacity, α-tocopherol and total phenols in blood and milk.
33041025	15	122	from	effects	2262:2268	arg1	capacity					2333:2340	total antioxidant capacity	2315:2340	total antioxidant capacity	2315:2340	Furthermore, we observed no effects of substituting soybean meal by spirulina on total antioxidant capacity, α-tocopherol and total phenols in blood and milk.
33041025	2	123	theme	dairy	415:419	arg1	cows					421:424	dairy cows	415:424	dairy cows	415:424	The suitability of spirulina (Arthrospira platensis), a fast growing and resource-efficient blue-green microalga, as a source of metabolizable protein for dairy cows is known, but its effects on milk antioxidants and sensory properties were never investigated.
33041025	12	124	theme	acid	1891:1894	arg1	proportion					1865:1874	a higher proportion	1856:1874	a higher proportion of γ-linolenic acid (0.057 vs. 0.038% of fatty acid methyl esters)	1856:1941	Similar to the spirulina lipids but far less pronounced, the milk fat from the spirulina-fed cows had a higher proportion of γ-linolenic acid (0.057 vs. 0.038% of fatty acid methyl esters) compared with milk fat from soybean meal-fed cows.
33041025	2	125	theme	blue-green	352:361	arg1	suitability					264:274	The suitability	260:274	The suitability of spirulina (Arthrospira platensis)	260:311	The suitability of spirulina (Arthrospira platensis), a fast growing and resource-efficient blue-green microalga, as a source of metabolizable protein for dairy cows is known, but its effects on milk antioxidants and sensory properties were never investigated.
33041025	2	125	theme	blue-green	352:361	arg1	microalga					363:371	a fast growing and resource-efficient blue-green microalga	314:371	a fast growing and resource-efficient blue-green microalga	314:371	The suitability of spirulina (Arthrospira platensis), a fast growing and resource-efficient blue-green microalga, as a source of metabolizable protein for dairy cows is known, but its effects on milk antioxidants and sensory properties were never investigated.
33041025	0	126	theme	substitution	15:26	arg1	Effects					0:6	Effects	0:6	Effects of the substitution of soybean meal by spirulina in a hay-based diet for dairy cows on milk composition and sensory perception.	0:134	Effects of the substitution of soybean meal by spirulina in a hay-based diet for dairy cows on milk composition and sensory perception.
33041025	2	127	theme	protein	403:409	arg1	source					379:384	a source	377:384	a source of metabolizable protein	377:409	The suitability of spirulina (Arthrospira platensis), a fast growing and resource-efficient blue-green microalga, as a source of metabolizable protein for dairy cows is known, but its effects on milk antioxidants and sensory properties were never investigated.
33041025	3	128	theme	beet	608:611	arg1	pulp					613:616	sugar beet pulp and wheat flakes	602:633	pulp	613:616	Twelve cows were allocated to 2 groups and fed hay-based diets complemented with sugar beet pulp and wheat flakes in individual feeding troughs.
33041025	8	129	theme	fat	1214:1216	arg1	contents					1202:1209	contents	1202:1209	contents	1202:1209	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	8	129	theme	fat	1214:1216	arg1	color					1191:1195	color	1191:1195	color	1191:1195	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	8	129	theme	fat	1214:1216	arg1	profile					1158:1164	fatty acid profile	1147:1164	fatty acid profile	1147:1164	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	8	129	theme	fat	1214:1216	arg1	properties					1179:1188	coagulation properties	1167:1188	coagulation properties	1167:1188	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	17	130	theme	favorable	2566:2574	arg1	constituents					2576:2587	nutritionally favorable constituents	2552:2587	nutritionally favorable constituents in milk	2552:2595	In conclusion, spirulina seems to be a promising protein source for dairy cows with certain improvements in nutritionally favorable constituents in milk and without side-effects on animal performance in the short term.
33041025	0	131	theme	soybean	31:37	arg1	meal					39:42	soybean meal	31:42	soybean meal	31:42	Effects of the substitution of soybean meal by spirulina in a hay-based diet for dairy cows on milk composition and sensory perception.
33041025	11	132	contain	had	1648:1650	arg1	milk					1615:1618	the milk	1611:1618	the milk from the spirulina-fed cows	1611:1646	However, the milk from the spirulina-fed cows had a higher content of β-carotene (0.207 vs. 0.135 μg/mL) and was more yellow (b* index: 14.9 vs. 13.8).
33041025	11	132	contain	had	1648:1650	arg1	index					1731:1735	b* index	1728:1735	b* index	1728:1735	However, the milk from the spirulina-fed cows had a higher content of β-carotene (0.207 vs. 0.135 μg/mL) and was more yellow (b* index: 14.9 vs. 13.8).
33041025	11	132	contain	had	1648:1650	arg1	yellow					1720:1725	yellow	1720:1725	yellow	1720:1725	However, the milk from the spirulina-fed cows had a higher content of β-carotene (0.207 vs. 0.135 μg/mL) and was more yellow (b* index: 14.9 vs. 13.8).
33041025	11	132	contain	had	1648:1650	arg2	content					1661:1667	a higher content	1652:1667	a higher content of β-carotene (0.207 vs. 0.135 μg/mL)	1652:1705	However, the milk from the spirulina-fed cows had a higher content of β-carotene (0.207 vs. 0.135 μg/mL) and was more yellow (b* index: 14.9 vs. 13.8).
33041025	1	133	theme	soybean	182:188	arg1	meal					190:193	soybean meal	182:193	soybean meal	182:193	The demand for protein sources alternative to soybean meal for supplementing forages low in metabolizable protein is large.
33041025	9	134	theme	bulk	1399:1402	arg1	samples					1409:1415	6 homogenized and pasteurized bulk milk samples	1369:1415	6 homogenized and pasteurized bulk milk samples per treatment	1369:1429	Triangle tests were performed by a trained sensory panel on 6 homogenized and pasteurized bulk milk samples per treatment.
33041025	8	135	theme	protein	1219:1225	arg1	contents					1202:1209	contents	1202:1209	contents	1202:1209	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	8	135	theme	protein	1219:1225	arg1	color					1191:1195	color	1191:1195	color	1191:1195	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	8	135	theme	protein	1219:1225	arg1	profile					1158:1164	fatty acid profile	1147:1164	fatty acid profile	1147:1164	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	8	135	theme	protein	1219:1225	arg1	properties					1179:1188	coagulation properties	1167:1188	coagulation properties	1167:1188	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	0	136	theme	dairy	81:85	arg1	cows					87:90	dairy cows	81:90	dairy cows on milk composition and sensory perception	81:133	Effects of the substitution of soybean meal by spirulina in a hay-based diet for dairy cows on milk composition and sensory perception.
33041025	7	137	theme	proximate	987:995	arg1	contents					997:1004	proximate contents	987:1004	proximate contents	987:1004	Feeds were analyzed for proximate contents, and blood plasma was analyzed for total antioxidant capacity and antioxidant contents (tocopherol, phenols).
33041025	14	138	theme	sensory	2160:2166	arg1	difference					2168:2177	No sensory difference	2157:2177	No sensory difference	2157:2177	No sensory difference was found between milk from the 2 experimental groups.
33041025	13	139	theme	C18:1	2040:2044	arg1	isomers					2052:2058	other C18:1 trans isomers	2034:2058	other C18:1 trans isomers	2034:2058	Also trans-11 C18:1 (vaccenic acid) and other C18:1 trans isomers were elevated, but otherwise the fatty acid profile resembled that of cows fed the control diet.
33041025	8	140	theme	lactose	1228:1234	arg1	contents					1202:1209	contents	1202:1209	contents	1202:1209	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	8	140	theme	lactose	1228:1234	arg1	color					1191:1195	color	1191:1195	color	1191:1195	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	8	140	theme	lactose	1228:1234	arg1	profile					1158:1164	fatty acid profile	1147:1164	fatty acid profile	1147:1164	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	8	140	theme	lactose	1228:1234	arg1	properties					1179:1188	coagulation properties	1167:1188	coagulation properties	1167:1188	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	8	141	theme	total	1237:1241	arg1	phenols					1243:1249	total phenols	1237:1249	total phenols	1237:1249	Milk samples were analyzed for fatty acid profile, coagulation properties, color, and contents of fat, protein, lactose, total phenols, lipophilic vitamins, and provitamins (e.g., β-carotene).
33041025	2	142	theme	platensis	302:310	arg1	suitability					264:274	The suitability	260:274	The suitability of spirulina (Arthrospira platensis)	260:311	The suitability of spirulina (Arthrospira platensis), a fast growing and resource-efficient blue-green microalga, as a source of metabolizable protein for dairy cows is known, but its effects on milk antioxidants and sensory properties were never investigated.
33041025	2	142	theme	platensis	302:310	arg1	microalga					363:371	a fast growing and resource-efficient blue-green microalga	314:371	a fast growing and resource-efficient blue-green microalga	314:371	The suitability of spirulina (Arthrospira platensis), a fast growing and resource-efficient blue-green microalga, as a source of metabolizable protein for dairy cows is known, but its effects on milk antioxidants and sensory properties were never investigated.
33041025	17	143	theme	certain	2528:2534	arg1	improvements					2536:2547	certain improvements	2528:2547	certain improvements in nutritionally favorable constituents in milk	2528:2595	In conclusion, spirulina seems to be a promising protein source for dairy cows with certain improvements in nutritionally favorable constituents in milk and without side-effects on animal performance in the short term.
33041025	10	144	theme	milk	1518:1521	arg1	yield					1523:1527	milk yield	1518:1527	milk yield	1518:1527	The substitution of soybean meal by spirulina in the diet did not affect feed intake, milk yield, milk fat, protein, or lactose contents compared with the control group.
33041025	1	145	theme	low	221:223	arg1	forages					213:219	forages	213:219	forages low in metabolizable protein	213:248	The demand for protein sources alternative to soybean meal for supplementing forages low in metabolizable protein is large.
34100698	10	0	dep	results	940:946	arg1	the					923:925	the	923:925	the	923:925	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	10	0	dep	results	940:946	arg1	basis					927:931	basis	927:931	basis	927:931	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	4	1	theme	0-5	523:525	arg1	%					526:526	%	526:526	%	526:526	Growth occurred at 4-42 °C (optimum 30-37 °C), at pH 5.0-9.0 (optimum pH 7.0) and in the presence of 0-5% NaCl (w/v) (optimum 1-3%).
34100698	2	2	theme	positive	113:120	arg1	bacterium					179:187	A novel Gram-stain positive, facultatively anaerobic, motile, irregularly rod-shaped bacterium	94:187	A novel Gram-stain positive, facultatively anaerobic, motile, irregularly rod-shaped bacterium	94:187	A novel Gram-stain positive, facultatively anaerobic, motile, irregularly rod-shaped bacterium, designated GY 10621T, was isolated from rhizosphere soil of Spartina alterniflora in Beihai City, Guangxi Province, PR China, and characterized using a polyphasic taxonomic approach.
34100698	10	3	theme	NBRC	1154:1157	arg1	16159T					1159:1164	Sanguibacter suarezii NBRC 16159T	1132:1164	Sanguibacter suarezii NBRC 16159T (96.39 %)	1132:1174	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	10	3	theme	NBRC	1154:1157	arg1	%					1173:1173	96.39 %	1167:1173	96.39 %	1167:1173	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	4	4	theme	 NaCl	527:531	arg1	presence					511:518	the presence	507:518	the presence of 0-5% NaCl (w/v) (optimum 1-3%)	507:552	Growth occurred at 4-42 °C (optimum 30-37 °C), at pH 5.0-9.0 (optimum pH 7.0) and in the presence of 0-5% NaCl (w/v) (optimum 1-3%).
34100698	9	5	theme	G+C	874:876	arg1	content					878:884	The DNA G+C content	866:884	The DNA G+C content of the type strain	866:903	The DNA G+C content of the type strain was 71.7 mol%.
34100698	9	5	theme	G+C	874:876	arg1	%					917:917	71.7 mol%	909:917	71.7 mol%	909:917	The DNA G+C content of the type strain was 71.7 mol%.
34100698	10	6	theme	Sanguibacter	1132:1143	arg1	16159T					1159:1164	Sanguibacter suarezii NBRC 16159T	1132:1164	Sanguibacter suarezii NBRC 16159T (96.39 %)	1132:1174	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	10	6	theme	Sanguibacter	1132:1143	arg1	%					1173:1173	96.39 %	1167:1173	96.39 %	1167:1173	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	10	7	theme	type	979:982	arg1	strain					984:989	the type strain	975:989	the type strain of a species with a validly published name with the highest similarity to GY 10621T	975:1073	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	10	7	theme	type	979:982	arg1	13155T					1102:1107	Flavimobilis soli KCTC 13155T	1079:1107	Flavimobilis soli KCTC 13155T (97.16 %)	1079:1117	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	6	8	theme	fatty	639:643	arg1	anteiso-C15 					656:667	anteiso-C15 	656:667	anteiso-C15 	656:667	The major cellular fatty acids were anteiso-C15 : 0 and C14 : 0.
34100698	6	8	theme	fatty	639:643	arg1	acids					645:649	The major cellular fatty acids	620:649	The major cellular fatty acids	620:649	The major cellular fatty acids were anteiso-C15 : 0 and C14 : 0.
34100698	12	9	theme	DNA-DNA	1443:1449	arg1	dDDH					1466:1469	dDDH	1466:1469	dDDH	1466:1469	massiliensis' Marseille-P3815) were 74.18-94.97 %, and the digital DNA-DNA hybridization (dDDH) values were 20.3-60.6 %.
34100698	12	9	theme	DNA-DNA	1443:1449	arg1	hybridization					1451:1463	digital DNA-DNA hybridization	1435:1463	the digital DNA-DNA hybridization (dDDH) values	1431:1477	massiliensis' Marseille-P3815) were 74.18-94.97 %, and the digital DNA-DNA hybridization (dDDH) values were 20.3-60.6 %.
34100698	11	10	theme	type	1355:1358	arg1	strains					1360:1366	the two aforementioned type strains	1332:1366	the two aforementioned type strains	1332:1366	The calculated results indicated that compared with GY 10621T, the average nucleotide identity (ANI) values of three strains closely related to GY 10621T (the two aforementioned type strains and 'S.
34100698	6	11	theme	major	624:628	arg1	anteiso-C15 					656:667	anteiso-C15 	656:667	anteiso-C15 	656:667	The major cellular fatty acids were anteiso-C15 : 0 and C14 : 0.
34100698	6	11	theme	major	624:628	arg1	acids					645:649	The major cellular fatty acids	620:649	The major cellular fatty acids	620:649	The major cellular fatty acids were anteiso-C15 : 0 and C14 : 0.
34100698	13	12	theme	genome-based	1531:1542	arg1	analysis					1562:1569	16S rRNA-based and genome-based phylogenetic tree analysis	1512:1569	analysis	1562:1569	The results of 16S rRNA-based and genome-based phylogenetic tree analysis indicated that GY 10621T should be assigned to the genus Flavimobilis.
34100698	6	13	dep	anteiso-C15 	656:667	arg1	C14 					676:679	C14 	676:679	C14 	676:679	The major cellular fatty acids were anteiso-C15 : 0 and C14 : 0.
34100698	6	13	dep	anteiso-C15 	656:667	arg1	 0					669:670	 0	669:670	 0	669:670	The major cellular fatty acids were anteiso-C15 : 0 and C14 : 0.
34100698	2	14	theme	novel	96:100	arg1	bacterium					179:187	A novel Gram-stain positive, facultatively anaerobic, motile, irregularly rod-shaped bacterium	94:187	A novel Gram-stain positive, facultatively anaerobic, motile, irregularly rod-shaped bacterium	94:187	A novel Gram-stain positive, facultatively anaerobic, motile, irregularly rod-shaped bacterium, designated GY 10621T, was isolated from rhizosphere soil of Spartina alterniflora in Beihai City, Guangxi Province, PR China, and characterized using a polyphasic taxonomic approach.
34100698	10	15	theme	species	996:1002	arg1	strain					984:989	the type strain	975:989	the type strain of a species with a validly published name with the highest similarity to GY 10621T	975:1073	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	10	15	theme	species	996:1002	arg1	13155T					1102:1107	Flavimobilis soli KCTC 13155T	1079:1107	Flavimobilis soli KCTC 13155T (97.16 %)	1079:1117	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	2	16	dep	Province	296:303	arg1	PR					306:307	PR	306:307	PR	306:307	A novel Gram-stain positive, facultatively anaerobic, motile, irregularly rod-shaped bacterium, designated GY 10621T, was isolated from rhizosphere soil of Spartina alterniflora in Beihai City, Guangxi Province, PR China, and characterized using a polyphasic taxonomic approach.
34100698	14	17	from	studies	1683:1689	arg1	evidence					1658:1665	evidence	1658:1665	evidence from polyphasic studies	1658:1689	On the basis of evidence from polyphasic studies, GY 10621T should be designated as representing a novel species of the genus Flavimobilis, for which the name Flavimobilis rhizosphaerae sp.
34100698	13	18	theme	genus	1622:1626	arg1	Flavimobilis					1628:1639	the genus Flavimobilis	1618:1639	the genus Flavimobilis	1618:1639	The results of 16S rRNA-based and genome-based phylogenetic tree analysis indicated that GY 10621T should be assigned to the genus Flavimobilis.
34100698	2	19	theme	Spartina	250:257	arg1	alterniflora					259:270	Spartina alterniflora	250:270	Spartina alterniflora	250:270	A novel Gram-stain positive, facultatively anaerobic, motile, irregularly rod-shaped bacterium, designated GY 10621T, was isolated from rhizosphere soil of Spartina alterniflora in Beihai City, Guangxi Province, PR China, and characterized using a polyphasic taxonomic approach.
34100698	13	20	theme	phylogenetic	1544:1555	arg1	analysis					1562:1569	16S rRNA-based and genome-based phylogenetic tree analysis	1512:1569	analysis	1562:1569	The results of 16S rRNA-based and genome-based phylogenetic tree analysis indicated that GY 10621T should be assigned to the genus Flavimobilis.
34100698	12	21	theme	digital	1435:1441	arg1	dDDH					1466:1469	dDDH	1466:1469	dDDH	1466:1469	massiliensis' Marseille-P3815) were 74.18-94.97 %, and the digital DNA-DNA hybridization (dDDH) values were 20.3-60.6 %.
34100698	12	21	theme	digital	1435:1441	arg1	hybridization					1451:1463	digital DNA-DNA hybridization	1435:1463	the digital DNA-DNA hybridization (dDDH) values	1431:1477	massiliensis' Marseille-P3815) were 74.18-94.97 %, and the digital DNA-DNA hybridization (dDDH) values were 20.3-60.6 %.
34100698	11	22	theme	GY	1229:1230	arg1	10621T					1232:1237	GY 10621T	1229:1237	GY 10621T	1229:1237	The calculated results indicated that compared with GY 10621T, the average nucleotide identity (ANI) values of three strains closely related to GY 10621T (the two aforementioned type strains and 'S.
34100698	11	22	theme	GY	1229:1230	arg1	ANI					1273:1275	ANI	1273:1275	ANI	1273:1275	The calculated results indicated that compared with GY 10621T, the average nucleotide identity (ANI) values of three strains closely related to GY 10621T (the two aforementioned type strains and 'S.
34100698	11	22	theme	GY	1229:1230	arg1	identity					1263:1270	the average nucleotide identity	1240:1270	the average nucleotide identity	1240:1270	The calculated results indicated that compared with GY 10621T, the average nucleotide identity (ANI) values of three strains closely related to GY 10621T (the two aforementioned type strains and 'S.
34100698	16	23	theme	1.17411T=KCTC	1887:1899	arg1	49515T					1901:1906	=CGMCC 1.17411T=KCTC 49515T	1880:1906	=CGMCC 1.17411T=KCTC 49515T	1880:1906	The type strain is GY 10621T (=CGMCC 1.17411T=KCTC 49515T).
34100698	16	23	theme	1.17411T=KCTC	1887:1899	arg1	10621T					1872:1877	GY 10621T	1869:1877	GY 10621T (=CGMCC 1.17411T=KCTC 49515T)	1869:1907	The type strain is GY 10621T (=CGMCC 1.17411T=KCTC 49515T).
34100698	11	24	dep	compared	1215:1222	arg1	'S					1372:1373	'S	1372:1373	'S	1372:1373	The calculated results indicated that compared with GY 10621T, the average nucleotide identity (ANI) values of three strains closely related to GY 10621T (the two aforementioned type strains and 'S.
34100698	11	24	dep	compared	1215:1222	arg1	strains					1360:1366	the two aforementioned type strains	1332:1366	the two aforementioned type strains	1332:1366	The calculated results indicated that compared with GY 10621T, the average nucleotide identity (ANI) values of three strains closely related to GY 10621T (the two aforementioned type strains and 'S.
34100698	14	25	dep	evidence	1658:1665	arg1	basis					1649:1653	basis	1649:1653	basis	1649:1653	On the basis of evidence from polyphasic studies, GY 10621T should be designated as representing a novel species of the genus Flavimobilis, for which the name Flavimobilis rhizosphaerae sp.
34100698	14	25	dep	evidence	1658:1665	arg1	the					1645:1647	the	1645:1647	the	1645:1647	On the basis of evidence from polyphasic studies, GY 10621T should be designated as representing a novel species of the genus Flavimobilis, for which the name Flavimobilis rhizosphaerae sp.
34100698	14	26	theme	rhizosphaerae	1814:1826	arg1	sp					1828:1829	the name Flavimobilis rhizosphaerae sp	1792:1829	the name Flavimobilis rhizosphaerae sp	1792:1829	On the basis of evidence from polyphasic studies, GY 10621T should be designated as representing a novel species of the genus Flavimobilis, for which the name Flavimobilis rhizosphaerae sp.
34100698	10	27	theme	published	1019:1027	arg1	name					1029:1032	a validly published name	1009:1032	a validly published name with the highest similarity to GY 10621T	1009:1073	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	2	28	attach	isolated	216:223	arg1	soil					242:245	rhizosphere soil	230:245	rhizosphere soil of Spartina alterniflora in Beihai City, Guangxi Province, PR China	230:313	A novel Gram-stain positive, facultatively anaerobic, motile, irregularly rod-shaped bacterium, designated GY 10621T, was isolated from rhizosphere soil of Spartina alterniflora in Beihai City, Guangxi Province, PR China, and characterized using a polyphasic taxonomic approach.
34100698	2	28	attach	isolated	216:223	arg2	bacterium					179:187	A novel Gram-stain positive, facultatively anaerobic, motile, irregularly rod-shaped bacterium	94:187	A novel Gram-stain positive, facultatively anaerobic, motile, irregularly rod-shaped bacterium	94:187	A novel Gram-stain positive, facultatively anaerobic, motile, irregularly rod-shaped bacterium, designated GY 10621T, was isolated from rhizosphere soil of Spartina alterniflora in Beihai City, Guangxi Province, PR China, and characterized using a polyphasic taxonomic approach.
34100698	4	29	theme	optimum	450:456	arg1	4-42 °C					441:447	4-42 °C	441:447	4-42 °C (optimum 30-37 °C)	441:466	Growth occurred at 4-42 °C (optimum 30-37 °C), at pH 5.0-9.0 (optimum pH 7.0) and in the presence of 0-5% NaCl (w/v) (optimum 1-3%).
34100698	4	29	theme	optimum	450:456	arg1	30-37 °C					458:465	optimum 30-37 °C	450:465	optimum 30-37 °C	450:465	Growth occurred at 4-42 °C (optimum 30-37 °C), at pH 5.0-9.0 (optimum pH 7.0) and in the presence of 0-5% NaCl (w/v) (optimum 1-3%).
34100698	14	30	theme	name	1796:1799	arg1	sp					1828:1829	the name Flavimobilis rhizosphaerae sp	1792:1829	the name Flavimobilis rhizosphaerae sp	1792:1829	On the basis of evidence from polyphasic studies, GY 10621T should be designated as representing a novel species of the genus Flavimobilis, for which the name Flavimobilis rhizosphaerae sp.
34100698	9	31	theme	strain	898:903	arg1	content					878:884	The DNA G+C content	866:884	The DNA G+C content of the type strain	866:903	The DNA G+C content of the type strain was 71.7 mol%.
34100698	9	31	theme	strain	898:903	arg1	%					917:917	71.7 mol%	909:917	71.7 mol%	909:917	The DNA G+C content of the type strain was 71.7 mol%.
34100698	1	32	theme	Spartina	71:78	arg1	alterniflora					80:91	Spartina alterniflora	71:91	Spartina alterniflora	71:91	nov., isolated from rhizosphere soil of Spartina alterniflora.
34100698	3	33	theme	GY	373:374	arg1	10621T					376:381	GY 10621T	373:381	GY 10621T	373:381	GY 10621T was positive for catalase and oxidase.
34100698	10	34	with	strain	984:989	arg1	name					1029:1032	a validly published name	1009:1032	a validly published name with the highest similarity to GY 10621T	1009:1073	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	10	35	theme	highest	1043:1049	arg1	similarity					1051:1060	the highest similarity	1039:1060	the highest similarity to GY 10621T	1039:1073	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	0	36	theme	Flavimobilis	0:11	arg1	sp					27:28	Flavimobilis rhizosphaerae sp	0:28	Flavimobilis rhizosphaerae sp.	0:29	Flavimobilis rhizosphaerae sp.
34100698	6	37	dep	 0	669:670	arg1	 0					681:682	 0	681:682	 0	681:682	The major cellular fatty acids were anteiso-C15 : 0 and C14 : 0.
34100698	10	38	theme	Flavimobilis	1079:1090	arg1	strain					984:989	the type strain	975:989	the type strain of a species with a validly published name with the highest similarity to GY 10621T	975:1073	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	10	38	theme	Flavimobilis	1079:1090	arg1	13155T					1102:1107	Flavimobilis soli KCTC 13155T	1079:1107	Flavimobilis soli KCTC 13155T (97.16 %)	1079:1117	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	10	38	theme	Flavimobilis	1079:1090	arg1	%					1116:1116	97.16 %	1110:1116	97.16 %	1110:1116	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	14	39	theme	genus	1762:1766	arg1	Flavimobilis					1768:1779	the genus Flavimobilis	1758:1779	the genus Flavimobilis	1758:1779	On the basis of evidence from polyphasic studies, GY 10621T should be designated as representing a novel species of the genus Flavimobilis, for which the name Flavimobilis rhizosphaerae sp.
34100698	13	40	theme	analysis	1562:1569	arg1	results					1501:1507	The results	1497:1507	The results of 16S rRNA-based and genome-based phylogenetic tree analysis	1497:1569	The results of 16S rRNA-based and genome-based phylogenetic tree analysis indicated that GY 10621T should be assigned to the genus Flavimobilis.
34100698	10	41	theme	rRNA	955:958	arg1	analysis					965:972	16S rRNA gene analysis	951:972	16S rRNA gene analysis	951:972	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	14	42	theme	polyphasic	1672:1681	arg1	studies					1683:1689	polyphasic studies	1672:1689	polyphasic studies	1672:1689	On the basis of evidence from polyphasic studies, GY 10621T should be designated as representing a novel species of the genus Flavimobilis, for which the name Flavimobilis rhizosphaerae sp.
34100698	8	43	theme	polar	743:747	arg1	lipids					749:754	The polar lipids	739:754	The polar lipids	739:754	The polar lipids included four phosphoglycolipids, four glycolipids, an unidentified lipid and six unidentified phospholipids.
34100698	4	44	theme	optimum	484:490	arg1	pH					472:473	pH 5.0-9.0	472:481	pH 5.0-9.0 (optimum pH 7.0)	472:498	Growth occurred at 4-42 °C (optimum 30-37 °C), at pH 5.0-9.0 (optimum pH 7.0) and in the presence of 0-5% NaCl (w/v) (optimum 1-3%).
34100698	4	44	theme	optimum	484:490	arg1	pH					492:493	optimum pH 7.0	484:497	optimum pH 7.0	484:497	Growth occurred at 4-42 °C (optimum 30-37 °C), at pH 5.0-9.0 (optimum pH 7.0) and in the presence of 0-5% NaCl (w/v) (optimum 1-3%).
34100698	14	45	theme	novel	1741:1745	arg1	species					1747:1753	a novel species	1739:1753	a novel species	1739:1753	On the basis of evidence from polyphasic studies, GY 10621T should be designated as representing a novel species of the genus Flavimobilis, for which the name Flavimobilis rhizosphaerae sp.
34100698	11	46	theme	strains	1294:1300	arg1	values					1278:1283	GY 10621T, the average nucleotide identity (ANI) values	1229:1283	GY 10621T, the average nucleotide identity (ANI) values of three strains closely related to GY 10621T	1229:1329	The calculated results indicated that compared with GY 10621T, the average nucleotide identity (ANI) values of three strains closely related to GY 10621T (the two aforementioned type strains and 'S.
34100698	9	47	theme	mol	914:916	arg1	%					917:917	71.7 mol%	909:917	71.7 mol%	909:917	The DNA G+C content of the type strain was 71.7 mol%.
34100698	9	47	theme	mol	914:916	arg1	content					878:884	The DNA G+C content	866:884	The DNA G+C content of the type strain	866:903	The DNA G+C content of the type strain was 71.7 mol%.
34100698	11	48	theme	aforementioned	1340:1353	arg1	strains					1360:1366	the two aforementioned type strains	1332:1366	the two aforementioned type strains	1332:1366	The calculated results indicated that compared with GY 10621T, the average nucleotide identity (ANI) values of three strains closely related to GY 10621T (the two aforementioned type strains and 'S.
34100698	2	49	theme	taxonomic	353:361	arg1	approach					363:370	a polyphasic taxonomic approach	340:370	a polyphasic taxonomic approach	340:370	A novel Gram-stain positive, facultatively anaerobic, motile, irregularly rod-shaped bacterium, designated GY 10621T, was isolated from rhizosphere soil of Spartina alterniflora in Beihai City, Guangxi Province, PR China, and characterized using a polyphasic taxonomic approach.
34100698	10	50	theme	analysis	965:972	arg1	results					940:946	the results	936:946	the results of 16S rRNA gene analysis	936:972	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	13	51	theme	rRNA-based	1516:1525	arg1	results					1501:1507	The results	1497:1507	The results of 16S rRNA-based and genome-based phylogenetic tree analysis	1497:1569	The results of 16S rRNA-based and genome-based phylogenetic tree analysis indicated that GY 10621T should be assigned to the genus Flavimobilis.
34100698	5	52	theme	main	559:562	arg1	MK-9					582:585	MK-9	582:585	MK-9	582:585	The main menaquinones were MK-9 (H4) (92.2 %) and MK-10 (7.8 %).
34100698	5	52	theme	main	559:562	arg1	menaquinones					564:575	The main menaquinones	555:575	The main menaquinones	555:575	The main menaquinones were MK-9 (H4) (92.2 %) and MK-10 (7.8 %).
34100698	10	53	theme	suarezii	1145:1152	arg1	16159T					1159:1164	Sanguibacter suarezii NBRC 16159T	1132:1164	Sanguibacter suarezii NBRC 16159T (96.39 %)	1132:1174	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	10	53	theme	suarezii	1145:1152	arg1	%					1173:1173	96.39 %	1167:1173	96.39 %	1167:1173	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	8	54	theme	unidentified	811:822	arg1	lipid					824:828	an unidentified lipid	808:828	an unidentified lipid	808:828	The polar lipids included four phosphoglycolipids, four glycolipids, an unidentified lipid and six unidentified phospholipids.
34100698	4	55	theme	%	526:526	arg1	%					551:551	optimum 1-3%	540:551	optimum 1-3%	540:551	Growth occurred at 4-42 °C (optimum 30-37 °C), at pH 5.0-9.0 (optimum pH 7.0) and in the presence of 0-5% NaCl (w/v) (optimum 1-3%).
34100698	4	55	theme	%	526:526	arg1	w/v					534:536	w/v	534:536	w/v	534:536	Growth occurred at 4-42 °C (optimum 30-37 °C), at pH 5.0-9.0 (optimum pH 7.0) and in the presence of 0-5% NaCl (w/v) (optimum 1-3%).
34100698	4	55	theme	%	526:526	arg1	 NaCl					527:531	0-5% NaCl	523:531	0-5% NaCl (w/v) (optimum 1-3%)	523:552	Growth occurred at 4-42 °C (optimum 30-37 °C), at pH 5.0-9.0 (optimum pH 7.0) and in the presence of 0-5% NaCl (w/v) (optimum 1-3%).
34100698	12	56	theme	hybridization	1451:1463	arg1	values					1472:1477	the digital DNA-DNA hybridization (dDDH) values	1431:1477	the digital DNA-DNA hybridization (dDDH) values	1431:1477	massiliensis' Marseille-P3815) were 74.18-94.97 %, and the digital DNA-DNA hybridization (dDDH) values were 20.3-60.6 %.
34100698	12	56	theme	hybridization	1451:1463	arg1	%					1494:1494	20.3-60.6 %	1484:1494	20.3-60.6 %	1484:1494	massiliensis' Marseille-P3815) were 74.18-94.97 %, and the digital DNA-DNA hybridization (dDDH) values were 20.3-60.6 %.
34100698	6	57	theme	cellular	630:637	arg1	anteiso-C15 					656:667	anteiso-C15 	656:667	anteiso-C15 	656:667	The major cellular fatty acids were anteiso-C15 : 0 and C14 : 0.
34100698	6	57	theme	cellular	630:637	arg1	acids					645:649	The major cellular fatty acids	620:649	The major cellular fatty acids	620:649	The major cellular fatty acids were anteiso-C15 : 0 and C14 : 0.
34100698	8	58	theme	unidentified	838:849	arg1	phospholipids					851:863	six unidentified phospholipids	834:863	six unidentified phospholipids	834:863	The polar lipids included four phosphoglycolipids, four glycolipids, an unidentified lipid and six unidentified phospholipids.
34100698	16	59	theme	=CGMCC	1880:1885	arg1	49515T					1901:1906	=CGMCC 1.17411T=KCTC 49515T	1880:1906	=CGMCC 1.17411T=KCTC 49515T	1880:1906	The type strain is GY 10621T (=CGMCC 1.17411T=KCTC 49515T).
34100698	16	59	theme	=CGMCC	1880:1885	arg1	10621T					1872:1877	GY 10621T	1869:1877	GY 10621T (=CGMCC 1.17411T=KCTC 49515T)	1869:1907	The type strain is GY 10621T (=CGMCC 1.17411T=KCTC 49515T).
34100698	4	60	theme	optimum	540:546	arg1	%					551:551	optimum 1-3%	540:551	optimum 1-3%	540:551	Growth occurred at 4-42 °C (optimum 30-37 °C), at pH 5.0-9.0 (optimum pH 7.0) and in the presence of 0-5% NaCl (w/v) (optimum 1-3%).
34100698	4	60	theme	optimum	540:546	arg1	 NaCl					527:531	0-5% NaCl	523:531	0-5% NaCl (w/v) (optimum 1-3%)	523:552	Growth occurred at 4-42 °C (optimum 30-37 °C), at pH 5.0-9.0 (optimum pH 7.0) and in the presence of 0-5% NaCl (w/v) (optimum 1-3%).
34100698	16	61	theme	type	1854:1857	arg1	strain					1859:1864	The type strain	1850:1864	The type strain	1850:1864	The type strain is GY 10621T (=CGMCC 1.17411T=KCTC 49515T).
34100698	16	61	theme	type	1854:1857	arg1	10621T					1872:1877	GY 10621T	1869:1877	GY 10621T (=CGMCC 1.17411T=KCTC 49515T)	1869:1907	The type strain is GY 10621T (=CGMCC 1.17411T=KCTC 49515T).
34100698	2	62	theme	rhizosphere	230:240	arg1	soil					242:245	rhizosphere soil	230:245	rhizosphere soil of Spartina alterniflora in Beihai City, Guangxi Province, PR China	230:313	A novel Gram-stain positive, facultatively anaerobic, motile, irregularly rod-shaped bacterium, designated GY 10621T, was isolated from rhizosphere soil of Spartina alterniflora in Beihai City, Guangxi Province, PR China, and characterized using a polyphasic taxonomic approach.
34100698	11	63	theme	10621T	1232:1237	arg1	values					1278:1283	GY 10621T, the average nucleotide identity (ANI) values	1229:1283	GY 10621T, the average nucleotide identity (ANI) values of three strains closely related to GY 10621T	1229:1329	The calculated results indicated that compared with GY 10621T, the average nucleotide identity (ANI) values of three strains closely related to GY 10621T (the two aforementioned type strains and 'S.
34100698	2	64	theme	alterniflora	259:270	arg1	soil					242:245	rhizosphere soil	230:245	rhizosphere soil of Spartina alterniflora in Beihai City, Guangxi Province, PR China	230:313	A novel Gram-stain positive, facultatively anaerobic, motile, irregularly rod-shaped bacterium, designated GY 10621T, was isolated from rhizosphere soil of Spartina alterniflora in Beihai City, Guangxi Province, PR China, and characterized using a polyphasic taxonomic approach.
34100698	10	65	theme	KCTC	1097:1100	arg1	strain					984:989	the type strain	975:989	the type strain of a species with a validly published name with the highest similarity to GY 10621T	975:1073	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	10	65	theme	KCTC	1097:1100	arg1	13155T					1102:1107	Flavimobilis soli KCTC 13155T	1079:1107	Flavimobilis soli KCTC 13155T (97.16 %)	1079:1117	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	10	65	theme	KCTC	1097:1100	arg1	%					1116:1116	97.16 %	1110:1116	97.16 %	1110:1116	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	11	66	theme	average	1244:1250	arg1	10621T					1232:1237	GY 10621T	1229:1237	GY 10621T	1229:1237	The calculated results indicated that compared with GY 10621T, the average nucleotide identity (ANI) values of three strains closely related to GY 10621T (the two aforementioned type strains and 'S.
34100698	11	66	theme	average	1244:1250	arg1	identity					1263:1270	the average nucleotide identity	1240:1270	the average nucleotide identity	1240:1270	The calculated results indicated that compared with GY 10621T, the average nucleotide identity (ANI) values of three strains closely related to GY 10621T (the two aforementioned type strains and 'S.
34100698	9	67	theme	DNA	870:872	arg1	content					878:884	The DNA G+C content	866:884	The DNA G+C content of the type strain	866:903	The DNA G+C content of the type strain was 71.7 mol%.
34100698	9	67	theme	DNA	870:872	arg1	%					917:917	71.7 mol%	909:917	71.7 mol%	909:917	The DNA G+C content of the type strain was 71.7 mol%.
34100698	11	68	theme	nucleotide	1252:1261	arg1	10621T					1232:1237	GY 10621T	1229:1237	GY 10621T	1229:1237	The calculated results indicated that compared with GY 10621T, the average nucleotide identity (ANI) values of three strains closely related to GY 10621T (the two aforementioned type strains and 'S.
34100698	11	68	theme	nucleotide	1252:1261	arg1	identity					1263:1270	the average nucleotide identity	1240:1270	the average nucleotide identity	1240:1270	The calculated results indicated that compared with GY 10621T, the average nucleotide identity (ANI) values of three strains closely related to GY 10621T (the two aforementioned type strains and 'S.
34100698	1	69	theme	rhizosphere	51:61	arg1	soil					63:66	rhizosphere soil	51:66	rhizosphere soil of Spartina alterniflora	51:91	nov., isolated from rhizosphere soil of Spartina alterniflora.
34100698	14	70	theme	Flavimobilis	1801:1812	arg1	sp					1828:1829	the name Flavimobilis rhizosphaerae sp	1792:1829	the name Flavimobilis rhizosphaerae sp	1792:1829	On the basis of evidence from polyphasic studies, GY 10621T should be designated as representing a novel species of the genus Flavimobilis, for which the name Flavimobilis rhizosphaerae sp.
34100698	9	71	theme	type	893:896	arg1	strain					898:903	the type strain	889:903	the type strain	889:903	The DNA G+C content of the type strain was 71.7 mol%.
34100698	10	72	theme	GY	1065:1066	arg1	10621T					1068:1073	GY 10621T	1065:1073	GY 10621T	1065:1073	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	13	73	theme	GY	1586:1587	arg1	10621T					1589:1594	GY 10621T	1586:1594	GY 10621T	1586:1594	The results of 16S rRNA-based and genome-based phylogenetic tree analysis indicated that GY 10621T should be assigned to the genus Flavimobilis.
34100698	0	74	theme	rhizosphaerae	13:25	arg1	sp					27:28	Flavimobilis rhizosphaerae sp	0:28	Flavimobilis rhizosphaerae sp.	0:29	Flavimobilis rhizosphaerae sp.
34100698	1	75	theme	alterniflora	80:91	arg1	soil					63:66	rhizosphere soil	51:66	rhizosphere soil of Spartina alterniflora	51:91	nov., isolated from rhizosphere soil of Spartina alterniflora.
34100698	10	76	theme	soli	1092:1095	arg1	strain					984:989	the type strain	975:989	the type strain of a species with a validly published name with the highest similarity to GY 10621T	975:1073	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	10	76	theme	soli	1092:1095	arg1	13155T					1102:1107	Flavimobilis soli KCTC 13155T	1079:1107	Flavimobilis soli KCTC 13155T (97.16 %)	1079:1117	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	10	76	theme	soli	1092:1095	arg1	%					1116:1116	97.16 %	1110:1116	97.16 %	1110:1116	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	14	77	theme	GY	1692:1693	arg1	10621T					1695:1700	GY 10621T	1692:1700	GY 10621T	1692:1700	On the basis of evidence from polyphasic studies, GY 10621T should be designated as representing a novel species of the genus Flavimobilis, for which the name Flavimobilis rhizosphaerae sp.
34100698	2	78	from	soil	242:245	arg1	China					309:313	China	309:313	China	309:313	A novel Gram-stain positive, facultatively anaerobic, motile, irregularly rod-shaped bacterium, designated GY 10621T, was isolated from rhizosphere soil of Spartina alterniflora in Beihai City, Guangxi Province, PR China, and characterized using a polyphasic taxonomic approach.
34100698	14	79	theme	Flavimobilis	1768:1779	arg1	species					1747:1753	a novel species	1739:1753	a novel species	1739:1753	On the basis of evidence from polyphasic studies, GY 10621T should be designated as representing a novel species of the genus Flavimobilis, for which the name Flavimobilis rhizosphaerae sp.
34100698	13	80	theme	tree	1557:1560	arg1	analysis					1562:1569	16S rRNA-based and genome-based phylogenetic tree analysis	1512:1569	analysis	1562:1569	The results of 16S rRNA-based and genome-based phylogenetic tree analysis indicated that GY 10621T should be assigned to the genus Flavimobilis.
34100698	11	81	theme	calculated	1181:1190	arg1	results					1192:1198	The calculated results	1177:1198	The calculated results	1177:1198	The calculated results indicated that compared with GY 10621T, the average nucleotide identity (ANI) values of three strains closely related to GY 10621T (the two aforementioned type strains and 'S.
34100698	2	82	dep	positive	113:120	arg1	motile					148:153	motile	148:153	motile	148:153	A novel Gram-stain positive, facultatively anaerobic, motile, irregularly rod-shaped bacterium, designated GY 10621T, was isolated from rhizosphere soil of Spartina alterniflora in Beihai City, Guangxi Province, PR China, and characterized using a polyphasic taxonomic approach.
34100698	2	82	dep	positive	113:120	arg1	rod-shaped					168:177	rod-shaped	168:177	rod-shaped	168:177	A novel Gram-stain positive, facultatively anaerobic, motile, irregularly rod-shaped bacterium, designated GY 10621T, was isolated from rhizosphere soil of Spartina alterniflora in Beihai City, Guangxi Province, PR China, and characterized using a polyphasic taxonomic approach.
34100698	2	82	dep	positive	113:120	arg1	anaerobic					137:145	anaerobic	137:145	anaerobic	137:145	A novel Gram-stain positive, facultatively anaerobic, motile, irregularly rod-shaped bacterium, designated GY 10621T, was isolated from rhizosphere soil of Spartina alterniflora in Beihai City, Guangxi Province, PR China, and characterized using a polyphasic taxonomic approach.
34100698	11	83	theme	GY	1321:1322	arg1	10621T					1324:1329	GY 10621T	1321:1329	GY 10621T	1321:1329	The calculated results indicated that compared with GY 10621T, the average nucleotide identity (ANI) values of three strains closely related to GY 10621T (the two aforementioned type strains and 'S.
34100698	10	84	theme	16S	951:953	arg1	analysis					965:972	16S rRNA gene analysis	951:972	16S rRNA gene analysis	951:972	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	10	85	with	name	1029:1032	arg1	similarity					1051:1060	the highest similarity	1039:1060	the highest similarity to GY 10621T	1039:1073	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	11	86	theme	related	1310:1316	arg1	strains					1294:1300	three strains	1288:1300	three strains closely related to GY 10621T	1288:1329	The calculated results indicated that compared with GY 10621T, the average nucleotide identity (ANI) values of three strains closely related to GY 10621T (the two aforementioned type strains and 'S.
34100698	10	87	theme	results	940:946	arg1	strain					984:989	the type strain	975:989	the type strain of a species with a validly published name with the highest similarity to GY 10621T	975:1073	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	10	87	theme	results	940:946	arg1	13155T					1102:1107	Flavimobilis soli KCTC 13155T	1079:1107	Flavimobilis soli KCTC 13155T (97.16 %)	1079:1117	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	10	87	theme	results	940:946	arg1	%					1116:1116	97.16 %	1110:1116	97.16 %	1110:1116	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34100698	2	88	theme	GY	201:202	arg1	10621T					204:209	GY 10621T	201:209	GY 10621T	201:209	A novel Gram-stain positive, facultatively anaerobic, motile, irregularly rod-shaped bacterium, designated GY 10621T, was isolated from rhizosphere soil of Spartina alterniflora in Beihai City, Guangxi Province, PR China, and characterized using a polyphasic taxonomic approach.
34100698	16	89	theme	GY	1869:1870	arg1	49515T					1901:1906	=CGMCC 1.17411T=KCTC 49515T	1880:1906	=CGMCC 1.17411T=KCTC 49515T	1880:1906	The type strain is GY 10621T (=CGMCC 1.17411T=KCTC 49515T).
34100698	16	89	theme	GY	1869:1870	arg1	strain					1859:1864	The type strain	1850:1864	The type strain	1850:1864	The type strain is GY 10621T (=CGMCC 1.17411T=KCTC 49515T).
34100698	16	89	theme	GY	1869:1870	arg1	10621T					1872:1877	GY 10621T	1869:1877	GY 10621T (=CGMCC 1.17411T=KCTC 49515T)	1869:1907	The type strain is GY 10621T (=CGMCC 1.17411T=KCTC 49515T).
34100698	12	90	dep	massiliensis	1376:1387	arg1	Marseille-P3815					1390:1404	Marseille-P3815	1390:1404	Marseille-P3815	1390:1404	massiliensis' Marseille-P3815) were 74.18-94.97 %, and the digital DNA-DNA hybridization (dDDH) values were 20.3-60.6 %.
34100698	2	91	theme	polyphasic	342:351	arg1	approach					363:370	a polyphasic taxonomic approach	340:370	a polyphasic taxonomic approach	340:370	A novel Gram-stain positive, facultatively anaerobic, motile, irregularly rod-shaped bacterium, designated GY 10621T, was isolated from rhizosphere soil of Spartina alterniflora in Beihai City, Guangxi Province, PR China, and characterized using a polyphasic taxonomic approach.
34100698	7	92	theme	type	711:714	arg1	l-Lys-Ser-d-Glu					721:735	l-Lys-Ser-d-Glu	721:735	l-Lys-Ser-d-Glu	721:735	The peptidoglycan was the type A4α (l-Lys-Ser-d-Glu).
34100698	7	92	theme	type	711:714	arg1	A4α					716:718	the type A4α	707:718	the type A4α (l-Lys-Ser-d-Glu)	707:736	The peptidoglycan was the type A4α (l-Lys-Ser-d-Glu).
34100698	7	92	theme	type	711:714	arg1	peptidoglycan					689:701	The peptidoglycan	685:701	The peptidoglycan	685:701	The peptidoglycan was the type A4α (l-Lys-Ser-d-Glu).
34100698	10	93	theme	gene	960:963	arg1	analysis					965:972	16S rRNA gene analysis	951:972	16S rRNA gene analysis	951:972	On the basis of the results of 16S rRNA gene analysis, the type strain of a species with a validly published name with the highest similarity to GY 10621T was Flavimobilis soli KCTC 13155T (97.16 %), followed by Sanguibacter suarezii NBRC 16159T (96.39 %).
34102271	7	0	from	inflammation	1404:1415	arg1	mice					1501:1504	C57bL6 mice	1494:1504	C57bL6 mice	1494:1504	The EEVM (30 and 100 mg/kg, p.o.) was tested against the Complete Freund Adjuvant (CFA)-induced paw persistent inflammation and Mycobacterium bovis (bacillus Calmette-Guerin - BCG)-induced pleurisy in C57bL6 mice.
34102271	7	1	theme	-induced	1380:1387	arg1	inflammation					1404:1415	the Complete Freund Adjuvant (CFA)-induced paw persistent inflammation	1346:1415	the Complete Freund Adjuvant (CFA)-induced paw persistent inflammation	1346:1415	The EEVM (30 and 100 mg/kg, p.o.) was tested against the Complete Freund Adjuvant (CFA)-induced paw persistent inflammation and Mycobacterium bovis (bacillus Calmette-Guerin - BCG)-induced pleurisy in C57bL6 mice.
34102271	2	2	theme	adverse	597:603	arg1	effects					605:611	adverse effects	597:611	adverse effects	597:611	The rheumatological and rheumatic diseases affect 0.3-1.0% of the world population and all long-term treatment with conventional medications lead to adverse effects.
34102271	11	3	theme	carrageenan-induced	2001:2019	arg1	pleurisy					2021:2028	carrageenan-induced pleurisy	2001:2028	carrageenan-induced pleurisy model	2001:2034	EEVM significantly inhibited leukocytes migration/proteins exudation in carrageenan-induced pleurisy model.
34102271	1	4	theme	general	383:389	arg1	aspects					404:410	the general inflammatory aspects	379:410	the general inflammatory aspects such as pains, injury and swelling	379:445	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	1	4	theme	general	383:389	arg1	pains					420:424	pains	420:424	pains	420:424	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	8	5	theme	EEVM	1543:1546	arg1	composition					1528:1538	The chemical composition	1515:1538	RESULTS The chemical composition of EEVM	1507:1546	RESULTS The chemical composition of EEVM showed 157.06 mg (GAE)/g in relation to phenolic compounds, 82.13 mg (RUE)/g in relation to flavonoids and 48.99 mg (TAE)/g in relation to tannins.
34102271	3	6	theme	ethanolic	719:727	arg1	extract					729:735	the ethanolic extract	715:735	the ethanolic extract from V. macrocarpa leaves (EEVM) in experimental models	715:791	AIM OF THE STUDY To investigate the chemical composition and the anti-inflammatory properties and of the ethanolic extract from V. macrocarpa leaves (EEVM) in experimental models.
34102271	8	7	theme	phenolic	1588:1595	arg1	compounds					1597:1605	phenolic compounds	1588:1605	phenolic compounds	1588:1605	RESULTS The chemical composition of EEVM showed 157.06 mg (GAE)/g in relation to phenolic compounds, 82.13 mg (RUE)/g in relation to flavonoids and 48.99 mg (TAE)/g in relation to tannins.
34102271	8	7	theme	phenolic	1588:1595	arg1	/g					1669:1670	48.99 mg (TAE)/g	1655:1670	48.99 mg (TAE)/g in relation to tannins	1655:1693	RESULTS The chemical composition of EEVM showed 157.06 mg (GAE)/g in relation to phenolic compounds, 82.13 mg (RUE)/g in relation to flavonoids and 48.99 mg (TAE)/g in relation to tannins.
34102271	8	7	theme	phenolic	1588:1595	arg1	/g					1622:1623	82.13 mg (RUE)/g	1608:1623	82.13 mg (RUE)/g in relation to flavonoids	1608:1649	RESULTS The chemical composition of EEVM showed 157.06 mg (GAE)/g in relation to phenolic compounds, 82.13 mg (RUE)/g in relation to flavonoids and 48.99 mg (TAE)/g in relation to tannins.
34102271	7	8	theme	Adjuvant	1366:1373	arg1	CFA					1376:1378	the Complete Freund Adjuvant (CFA)	1346:1379	the Complete Freund Adjuvant (CFA)	1346:1379	The EEVM (30 and 100 mg/kg, p.o.) was tested against the Complete Freund Adjuvant (CFA)-induced paw persistent inflammation and Mycobacterium bovis (bacillus Calmette-Guerin - BCG)-induced pleurisy in C57bL6 mice.
34102271	12	9	from	parameters	2097:2106	arg1	models					2123:2128	BCG and CFA models	2111:2128	BCG and CFA models	2111:2128	The daily administration of EEVM inhibited the inflammatory parameters in BCG and CFA models.
34102271	7	10	dep	EEVM	1297:1300	arg1	p.o.					1321:1324	p.o.	1321:1324	p.o.	1321:1324	The EEVM (30 and 100 mg/kg, p.o.) was tested against the Complete Freund Adjuvant (CFA)-induced paw persistent inflammation and Mycobacterium bovis (bacillus Calmette-Guerin - BCG)-induced pleurisy in C57bL6 mice.
34102271	7	10	dep	EEVM	1297:1300	arg1	100 mg/kg					1310:1318	100 mg/kg	1310:1318	100 mg/kg	1310:1318	The EEVM (30 and 100 mg/kg, p.o.) was tested against the Complete Freund Adjuvant (CFA)-induced paw persistent inflammation and Mycobacterium bovis (bacillus Calmette-Guerin - BCG)-induced pleurisy in C57bL6 mice.
34102271	7	10	dep	EEVM	1297:1300	arg1	30					1303:1304	30	1303:1304	30	1303:1304	The EEVM (30 and 100 mg/kg, p.o.) was tested against the Complete Freund Adjuvant (CFA)-induced paw persistent inflammation and Mycobacterium bovis (bacillus Calmette-Guerin - BCG)-induced pleurisy in C57bL6 mice.
34102271	7	11	theme	Complete	1350:1357	arg1	CFA					1376:1378	the Complete Freund Adjuvant (CFA)	1346:1379	the Complete Freund Adjuvant (CFA)	1346:1379	The EEVM (30 and 100 mg/kg, p.o.) was tested against the Complete Freund Adjuvant (CFA)-induced paw persistent inflammation and Mycobacterium bovis (bacillus Calmette-Guerin - BCG)-induced pleurisy in C57bL6 mice.
34102271	11	12	theme	leukocytes	1958:1967	arg1	exudation					1988:1996	leukocytes migration/proteins exudation	1958:1996	leukocytes migration/proteins exudation	1958:1996	EEVM significantly inhibited leukocytes migration/proteins exudation in carrageenan-induced pleurisy model.
34102271	10	13	theme	MTT	1836:1838	arg1	assay					1840:1844	MTT assay	1836:1844	MTT assay	1836:1844	EEVM did not present cytotoxicity in MTT assay, however EEVM reduced phagocytic neutrophils activity at all tested concentration.
34102271	6	14	theme	carrageenan-induced	1249:1267	arg1	pleurisy					1269:1276	carrageenan-induced pleurisy	1249:1276	carrageenan-induced pleurisy in Swiss mice	1249:1290	For in vivo models, the EEVM (10, 30, 100, and 300 mg/kg) was orally administered (p.o.) for inflammatory evaluation in carrageenan-induced pleurisy in Swiss mice.
34102271	8	15	from	/g	1669:1670	arg1	relation					1628:1635	relation	1628:1635	relation to flavonoids	1628:1649	RESULTS The chemical composition of EEVM showed 157.06 mg (GAE)/g in relation to phenolic compounds, 82.13 mg (RUE)/g in relation to flavonoids and 48.99 mg (TAE)/g in relation to tannins.
34102271	8	15	from	/g	1669:1670	arg1	relation					1675:1682	relation	1675:1682	relation to tannins	1675:1693	RESULTS The chemical composition of EEVM showed 157.06 mg (GAE)/g in relation to phenolic compounds, 82.13 mg (RUE)/g in relation to flavonoids and 48.99 mg (TAE)/g in relation to tannins.
34102271	4	16	theme	mass	948:951	arg1	spectrometry					953:964	mass spectrometry	948:964	mass spectrometry	948:964	MATERIAL AND METHODS EEVM was chemically analyzed by spectrophotometry and the compounds characterization was performed by nuclear magnetic resonance and mass spectrometry.
34102271	12	17	theme	daily	2041:2045	arg1	administration					2047:2060	The daily administration	2037:2060	The daily administration of EEVM	2037:2068	The daily administration of EEVM inhibited the inflammatory parameters in BCG and CFA models.
34102271	10	18	theme	phagocytic	1868:1877	arg1	activity					1891:1898	phagocytic neutrophils activity	1868:1898	phagocytic neutrophils activity	1868:1898	EEVM did not present cytotoxicity in MTT assay, however EEVM reduced phagocytic neutrophils activity at all tested concentration.
34102271	13	19	theme	inflammatory	2246:2257	arg1	diseases					2259:2266	inflammatory diseases	2246:2266	inflammatory diseases	2246:2266	CONCLUSIONS The present study showed anti-inflammatory features of EEVM (V. macrocarpa) as a natural agent against inflammatory diseases.
34102271	2	20	theme	conventional	564:575	arg1	medications					577:587	conventional medications	564:587	conventional medications lead to adverse effects	564:611	The rheumatological and rheumatic diseases affect 0.3-1.0% of the world population and all long-term treatment with conventional medications lead to adverse effects.
34102271	6	21	from	evaluation	1235:1244	arg1	pleurisy					1269:1276	carrageenan-induced pleurisy	1249:1276	carrageenan-induced pleurisy in Swiss mice	1249:1290	For in vivo models, the EEVM (10, 30, 100, and 300 mg/kg) was orally administered (p.o.) for inflammatory evaluation in carrageenan-induced pleurisy in Swiss mice.
34102271	1	22	theme	inflammatory	391:402	arg1	aspects					404:410	the general inflammatory aspects	379:410	the general inflammatory aspects such as pains, injury and swelling	379:445	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	1	22	theme	inflammatory	391:402	arg1	pains					420:424	pains	420:424	pains	420:424	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	4	23	theme	nuclear	917:923	arg1	resonance					934:942	nuclear magnetic resonance	917:942	nuclear magnetic resonance	917:942	MATERIAL AND METHODS EEVM was chemically analyzed by spectrophotometry and the compounds characterization was performed by nuclear magnetic resonance and mass spectrometry.
34102271	7	24	theme	C57bL6	1494:1499	arg1	mice					1501:1504	C57bL6 mice	1494:1504	C57bL6 mice	1494:1504	The EEVM (30 and 100 mg/kg, p.o.) was tested against the Complete Freund Adjuvant (CFA)-induced paw persistent inflammation and Mycobacterium bovis (bacillus Calmette-Guerin - BCG)-induced pleurisy in C57bL6 mice.
34102271	3	25	theme	experimental	773:784	arg1	models					786:791	experimental models	773:791	experimental models	773:791	AIM OF THE STUDY To investigate the chemical composition and the anti-inflammatory properties and of the ethanolic extract from V. macrocarpa leaves (EEVM) in experimental models.
34102271	5	26	theme	3	1105:1105	arg1	tests					1122:1126	phagocytic activity (1, 3, and 10 μg/ml) tests	1081:1126	phagocytic activity (1, 3, and 10 μg/ml) tests	1081:1126	EEVM was evaluated in methylthiazolyldiphenyl-tetrazolium bromide (MTT) (3, 10, 30, and 90 μg/ml) and neutrophils phagocytic activity (1, 3, and 10 μg/ml) tests.
34102271	1	27	from	macrocarpa	193:202	arg1	heartwood					169:177	heartwood	169:177	heartwood from Vatairea macrocarpa (Benth)	169:210	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	1	27	from	macrocarpa	193:202	arg1	medicine					144:151	Brazilian traditional folk medicine	117:151	Brazilian traditional folk medicine	117:151	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	13	28	theme	EEVM	2198:2201	arg1	agent					2232:2236	a natural agent	2222:2236	a natural agent against inflammatory diseases	2222:2266	CONCLUSIONS The present study showed anti-inflammatory features of EEVM (V. macrocarpa) as a natural agent against inflammatory diseases.
34102271	13	28	theme	EEVM	2198:2201	arg1	features					2186:2193	anti-inflammatory features	2168:2193	anti-inflammatory features of EEVM (V. macrocarpa)	2168:2217	CONCLUSIONS The present study showed anti-inflammatory features of EEVM (V. macrocarpa) as a natural agent against inflammatory diseases.
34102271	2	29	theme	rheumatic	472:480	arg1	diseases					482:489	The rheumatological and rheumatic diseases	448:489	diseases	482:489	The rheumatological and rheumatic diseases affect 0.3-1.0% of the world population and all long-term treatment with conventional medications lead to adverse effects.
34102271	13	30	theme	anti-inflammatory	2168:2184	arg1	agent					2232:2236	a natural agent	2222:2236	a natural agent against inflammatory diseases	2222:2266	CONCLUSIONS The present study showed anti-inflammatory features of EEVM (V. macrocarpa) as a natural agent against inflammatory diseases.
34102271	13	30	theme	anti-inflammatory	2168:2184	arg1	features					2186:2193	anti-inflammatory features	2168:2193	anti-inflammatory features of EEVM (V. macrocarpa)	2168:2217	CONCLUSIONS The present study showed anti-inflammatory features of EEVM (V. macrocarpa) as a natural agent against inflammatory diseases.
34102271	3	31	dep	macrocarpa	745:754	arg1	leaves					756:761	leaves	756:761	leaves	756:761	AIM OF THE STUDY To investigate the chemical composition and the anti-inflammatory properties and of the ethanolic extract from V. macrocarpa leaves (EEVM) in experimental models.
34102271	1	32	theme	Vatairea	184:191	arg1	Benth					205:209	Benth	205:209	Benth	205:209	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	1	32	theme	Vatairea	184:191	arg1	macrocarpa					193:202	Vatairea macrocarpa	184:202	Vatairea macrocarpa (Benth)	184:210	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	1	33	dep	the	154:156	arg1	leaves					158:163	leaves	158:163	leaves	158:163	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	1	33	dep	the	154:156	arg1	Angelim-of-Cerrado					219:236	Angelim-of-Cerrado	219:236	Angelim-of-Cerrado	219:236	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	5	34	theme	activity	1092:1099	arg1	tests					1122:1126	phagocytic activity (1, 3, and 10 μg/ml) tests	1081:1126	phagocytic activity (1, 3, and 10 μg/ml) tests	1081:1126	EEVM was evaluated in methylthiazolyldiphenyl-tetrazolium bromide (MTT) (3, 10, 30, and 90 μg/ml) and neutrophils phagocytic activity (1, 3, and 10 μg/ml) tests.
34102271	5	35	theme	1	1102:1102	arg1	tests					1122:1126	phagocytic activity (1, 3, and 10 μg/ml) tests	1081:1126	phagocytic activity (1, 3, and 10 μg/ml) tests	1081:1126	EEVM was evaluated in methylthiazolyldiphenyl-tetrazolium bromide (MTT) (3, 10, 30, and 90 μg/ml) and neutrophils phagocytic activity (1, 3, and 10 μg/ml) tests.
34102271	2	36	with	%	505:505	arg1	medications					577:587	conventional medications	564:587	conventional medications lead to adverse effects	564:611	The rheumatological and rheumatic diseases affect 0.3-1.0% of the world population and all long-term treatment with conventional medications lead to adverse effects.
34102271	13	37	dep	CONCLUSIONS	2131:2141	arg1	showed					2161:2166	showed	2161:2166	showed anti-inflammatory features of EEVM (V. macrocarpa) as a natural agent against inflammatory diseases	2161:2266	CONCLUSIONS The present study showed anti-inflammatory features of EEVM (V. macrocarpa) as a natural agent against inflammatory diseases.
34102271	3	38	from	macrocarpa	745:754	arg1	AIM					614:616	AIM	614:616	AIM OF THE STUDY To investigate the chemical composition and the anti-inflammatory properties and of the ethanolic extract from V. macrocarpa leaves (EEVM) in experimental models.	614:792	AIM OF THE STUDY To investigate the chemical composition and the anti-inflammatory properties and of the ethanolic extract from V. macrocarpa leaves (EEVM) in experimental models.
34102271	3	38	from	macrocarpa	745:754	arg1	models					786:791	experimental models	773:791	experimental models	773:791	AIM OF THE STUDY To investigate the chemical composition and the anti-inflammatory properties and of the ethanolic extract from V. macrocarpa leaves (EEVM) in experimental models.
34102271	3	38	from	macrocarpa	745:754	arg1	extract					729:735	the ethanolic extract	715:735	the ethanolic extract from V. macrocarpa leaves (EEVM) in experimental models	715:791	AIM OF THE STUDY To investigate the chemical composition and the anti-inflammatory properties and of the ethanolic extract from V. macrocarpa leaves (EEVM) in experimental models.
34102271	0	39	theme	Anti-inflammatory	0:16	arg1	properties					18:27	Anti-inflammatory properties	0:27	Anti-inflammatory properties of ethanolic extract from Vatairea macrocarpa	0:73	Anti-inflammatory properties of ethanolic extract from Vatairea macrocarpa leaves.
34102271	1	40	theme	Brazilian	117:125	arg1	the					154:156	the	154:156	the	154:156	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	1	40	theme	Brazilian	117:125	arg1	heartwood					169:177	heartwood	169:177	heartwood from Vatairea macrocarpa (Benth)	169:210	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	1	40	theme	Brazilian	117:125	arg1	medicine					144:151	Brazilian traditional folk medicine	117:151	Brazilian traditional folk medicine	117:151	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	8	41	from	/g	1570:1571	arg1	relation					1576:1583	relation	1576:1583	relation to phenolic compounds, 82.13 mg (RUE)/g in relation to flavonoids and 48.99 mg (TAE)/g in relation to tannins	1576:1693	RESULTS The chemical composition of EEVM showed 157.06 mg (GAE)/g in relation to phenolic compounds, 82.13 mg (RUE)/g in relation to flavonoids and 48.99 mg (TAE)/g in relation to tannins.
34102271	6	42	from	pleurisy	1269:1276	arg1	mice					1287:1290	Swiss mice	1281:1290	Swiss mice	1281:1290	For in vivo models, the EEVM (10, 30, 100, and 300 mg/kg) was orally administered (p.o.) for inflammatory evaluation in carrageenan-induced pleurisy in Swiss mice.
34102271	1	43	theme	folk	139:142	arg1	the					154:156	the	154:156	the	154:156	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	1	43	theme	folk	139:142	arg1	heartwood					169:177	heartwood	169:177	heartwood from Vatairea macrocarpa (Benth)	169:210	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	1	43	theme	folk	139:142	arg1	medicine					144:151	Brazilian traditional folk medicine	117:151	Brazilian traditional folk medicine	117:151	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	2	44	theme	rheumatological	452:466	arg1	diseases					482:489	The rheumatological and rheumatic diseases	448:489	diseases	482:489	The rheumatological and rheumatic diseases affect 0.3-1.0% of the world population and all long-term treatment with conventional medications lead to adverse effects.
34102271	0	45	theme	extract	42:48	arg1	properties					18:27	Anti-inflammatory properties	0:27	Anti-inflammatory properties of ethanolic extract from Vatairea macrocarpa	0:73	Anti-inflammatory properties of ethanolic extract from Vatairea macrocarpa leaves.
34102271	1	46	used	used	261:264	arg2	remedy					269:274	remedy	269:274	remedy after cold maceration	269:296	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	1	46	used	used	261:264	arg2	RELEVANCE					104:112	ETHNOPHARMACOLOGICAL RELEVANCE	83:112	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family)	83:255	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	3	47	theme	STUDY	625:629	arg1	AIM					614:616	AIM	614:616	AIM OF THE STUDY To investigate the chemical composition and the anti-inflammatory properties and of the ethanolic extract from V. macrocarpa leaves (EEVM) in experimental models.	614:792	AIM OF THE STUDY To investigate the chemical composition and the anti-inflammatory properties and of the ethanolic extract from V. macrocarpa leaves (EEVM) in experimental models.
34102271	0	48	theme	Vatairea	55:62	arg1	macrocarpa					64:73	Vatairea macrocarpa	55:73	Vatairea macrocarpa	55:73	Anti-inflammatory properties of ethanolic extract from Vatairea macrocarpa leaves.
34102271	7	49	from	pleurisy	1482:1489	arg1	mice					1501:1504	C57bL6 mice	1494:1504	C57bL6 mice	1494:1504	The EEVM (30 and 100 mg/kg, p.o.) was tested against the Complete Freund Adjuvant (CFA)-induced paw persistent inflammation and Mycobacterium bovis (bacillus Calmette-Guerin - BCG)-induced pleurisy in C57bL6 mice.
34102271	12	50	theme	inflammatory	2084:2095	arg1	parameters					2097:2106	the inflammatory parameters	2080:2106	the inflammatory parameters in BCG and CFA models	2080:2128	The daily administration of EEVM inhibited the inflammatory parameters in BCG and CFA models.
34102271	7	51	theme	paw	1389:1391	arg1	inflammation					1404:1415	the Complete Freund Adjuvant (CFA)-induced paw persistent inflammation	1346:1415	the Complete Freund Adjuvant (CFA)-induced paw persistent inflammation	1346:1415	The EEVM (30 and 100 mg/kg, p.o.) was tested against the Complete Freund Adjuvant (CFA)-induced paw persistent inflammation and Mycobacterium bovis (bacillus Calmette-Guerin - BCG)-induced pleurisy in C57bL6 mice.
34102271	3	52	theme	chemical	650:657	arg1	composition					659:669	the chemical composition	646:669	the chemical composition	646:669	AIM OF THE STUDY To investigate the chemical composition and the anti-inflammatory properties and of the ethanolic extract from V. macrocarpa leaves (EEVM) in experimental models.
34102271	1	53	from	RELEVANCE	104:112	arg1	the					154:156	the	154:156	the	154:156	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	1	53	from	RELEVANCE	104:112	arg1	heartwood					169:177	heartwood	169:177	heartwood from Vatairea macrocarpa (Benth)	169:210	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	1	53	from	RELEVANCE	104:112	arg1	medicine					144:151	Brazilian traditional folk medicine	117:151	Brazilian traditional folk medicine	117:151	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	8	54	theme	chemical	1519:1526	arg1	composition					1528:1538	The chemical composition	1515:1538	RESULTS The chemical composition of EEVM	1507:1546	RESULTS The chemical composition of EEVM showed 157.06 mg (GAE)/g in relation to phenolic compounds, 82.13 mg (RUE)/g in relation to flavonoids and 48.99 mg (TAE)/g in relation to tannins.
34102271	3	55	theme	anti-inflammatory	679:695	arg1	properties					697:706	the anti-inflammatory properties	675:706	the anti-inflammatory properties	675:706	AIM OF THE STUDY To investigate the chemical composition and the anti-inflammatory properties and of the ethanolic extract from V. macrocarpa leaves (EEVM) in experimental models.
34102271	7	56	theme	Freund	1359:1364	arg1	CFA					1376:1378	the Complete Freund Adjuvant (CFA)	1346:1379	the Complete Freund Adjuvant (CFA)	1346:1379	The EEVM (30 and 100 mg/kg, p.o.) was tested against the Complete Freund Adjuvant (CFA)-induced paw persistent inflammation and Mycobacterium bovis (bacillus Calmette-Guerin - BCG)-induced pleurisy in C57bL6 mice.
34102271	4	57	theme	compounds	873:881	arg1	characterization					883:898	the compounds characterization	869:898	the compounds characterization	869:898	MATERIAL AND METHODS EEVM was chemically analyzed by spectrophotometry and the compounds characterization was performed by nuclear magnetic resonance and mass spectrometry.
34102271	3	58	theme	extract	729:735	arg1	AIM					614:616	AIM	614:616	AIM OF THE STUDY To investigate the chemical composition and the anti-inflammatory properties and of the ethanolic extract from V. macrocarpa leaves (EEVM) in experimental models.	614:792	AIM OF THE STUDY To investigate the chemical composition and the anti-inflammatory properties and of the ethanolic extract from V. macrocarpa leaves (EEVM) in experimental models.
34102271	4	59	dep	MATERIAL	794:801	arg1	EEVM					815:818	EEVM	815:818	EEVM	815:818	MATERIAL AND METHODS EEVM was chemically analyzed by spectrophotometry and the compounds characterization was performed by nuclear magnetic resonance and mass spectrometry.
34102271	6	60	dep	administered	1198:1209	arg1	p.o.					1212:1215	p.o.	1212:1215	p.o.	1212:1215	For in vivo models, the EEVM (10, 30, 100, and 300 mg/kg) was orally administered (p.o.) for inflammatory evaluation in carrageenan-induced pleurisy in Swiss mice.
34102271	6	61	theme	in	1133:1134	arg1	models					1141:1146	in vivo models	1133:1146	in vivo models	1133:1146	For in vivo models, the EEVM (10, 30, 100, and 300 mg/kg) was orally administered (p.o.) for inflammatory evaluation in carrageenan-induced pleurisy in Swiss mice.
34102271	8	62	from	/g	1622:1623	arg1	relation					1628:1635	relation	1628:1635	relation to flavonoids	1628:1649	RESULTS The chemical composition of EEVM showed 157.06 mg (GAE)/g in relation to phenolic compounds, 82.13 mg (RUE)/g in relation to flavonoids and 48.99 mg (TAE)/g in relation to tannins.
34102271	8	62	from	/g	1622:1623	arg1	relation					1675:1682	relation	1675:1682	relation to tannins	1675:1693	RESULTS The chemical composition of EEVM showed 157.06 mg (GAE)/g in relation to phenolic compounds, 82.13 mg (RUE)/g in relation to flavonoids and 48.99 mg (TAE)/g in relation to tannins.
34102271	8	63	theme	82.13 mg	1608:1615	arg1	compounds					1597:1605	phenolic compounds	1588:1605	phenolic compounds	1588:1605	RESULTS The chemical composition of EEVM showed 157.06 mg (GAE)/g in relation to phenolic compounds, 82.13 mg (RUE)/g in relation to flavonoids and 48.99 mg (TAE)/g in relation to tannins.
34102271	8	63	theme	82.13 mg	1608:1615	arg1	/g					1622:1623	82.13 mg (RUE)/g	1608:1623	82.13 mg (RUE)/g in relation to flavonoids	1608:1649	RESULTS The chemical composition of EEVM showed 157.06 mg (GAE)/g in relation to phenolic compounds, 82.13 mg (RUE)/g in relation to flavonoids and 48.99 mg (TAE)/g in relation to tannins.
34102271	13	64	theme	natural	2224:2230	arg1	agent					2232:2236	a natural agent	2222:2236	a natural agent against inflammatory diseases	2222:2266	CONCLUSIONS The present study showed anti-inflammatory features of EEVM (V. macrocarpa) as a natural agent against inflammatory diseases.
34102271	13	64	theme	natural	2224:2230	arg1	features					2186:2193	anti-inflammatory features	2168:2193	anti-inflammatory features of EEVM (V. macrocarpa)	2168:2217	CONCLUSIONS The present study showed anti-inflammatory features of EEVM (V. macrocarpa) as a natural agent against inflammatory diseases.
34102271	4	65	theme	magnetic	925:932	arg1	resonance					934:942	nuclear magnetic resonance	917:942	nuclear magnetic resonance	917:942	MATERIAL AND METHODS EEVM was chemically analyzed by spectrophotometry and the compounds characterization was performed by nuclear magnetic resonance and mass spectrometry.
34102271	13	66	theme	V.	2204:2205	arg1	EEVM					2198:2201	EEVM	2198:2201	EEVM (V. macrocarpa)	2198:2217	CONCLUSIONS The present study showed anti-inflammatory features of EEVM (V. macrocarpa) as a natural agent against inflammatory diseases.
34102271	13	66	theme	V.	2204:2205	arg1	macrocarpa					2207:2216	V. macrocarpa	2204:2216	V. macrocarpa	2204:2216	CONCLUSIONS The present study showed anti-inflammatory features of EEVM (V. macrocarpa) as a natural agent against inflammatory diseases.
34102271	6	67	dep	in	1133:1134	arg1	vivo					1136:1139	vivo	1136:1139	vivo	1136:1139	For in vivo models, the EEVM (10, 30, 100, and 300 mg/kg) was orally administered (p.o.) for inflammatory evaluation in carrageenan-induced pleurisy in Swiss mice.
34102271	3	68	theme	V.	742:743	arg1	macrocarpa					745:754	V. macrocarpa	742:754	V. macrocarpa leaves (EEVM) in experimental models	742:791	AIM OF THE STUDY To investigate the chemical composition and the anti-inflammatory properties and of the ethanolic extract from V. macrocarpa leaves (EEVM) in experimental models.
34102271	3	68	theme	V.	742:743	arg1	EEVM					764:767	EEVM	764:767	EEVM	764:767	AIM OF THE STUDY To investigate the chemical composition and the anti-inflammatory properties and of the ethanolic extract from V. macrocarpa leaves (EEVM) in experimental models.
34102271	8	69	theme	RUE	1618:1620	arg1	compounds					1597:1605	phenolic compounds	1588:1605	phenolic compounds	1588:1605	RESULTS The chemical composition of EEVM showed 157.06 mg (GAE)/g in relation to phenolic compounds, 82.13 mg (RUE)/g in relation to flavonoids and 48.99 mg (TAE)/g in relation to tannins.
34102271	8	69	theme	RUE	1618:1620	arg1	/g					1622:1623	82.13 mg (RUE)/g	1608:1623	82.13 mg (RUE)/g in relation to flavonoids	1608:1649	RESULTS The chemical composition of EEVM showed 157.06 mg (GAE)/g in relation to phenolic compounds, 82.13 mg (RUE)/g in relation to flavonoids and 48.99 mg (TAE)/g in relation to tannins.
34102271	2	70	theme	world	514:518	arg1	population					520:529	the world population	510:529	the world population	510:529	The rheumatological and rheumatic diseases affect 0.3-1.0% of the world population and all long-term treatment with conventional medications lead to adverse effects.
34102271	6	71	theme	inflammatory	1222:1233	arg1	evaluation					1235:1244	inflammatory evaluation	1222:1244	inflammatory evaluation in carrageenan-induced pleurisy in Swiss mice	1222:1290	For in vivo models, the EEVM (10, 30, 100, and 300 mg/kg) was orally administered (p.o.) for inflammatory evaluation in carrageenan-induced pleurisy in Swiss mice.
34102271	5	72	theme	phagocytic	1081:1090	arg1	tests					1122:1126	phagocytic activity (1, 3, and 10 μg/ml) tests	1081:1126	phagocytic activity (1, 3, and 10 μg/ml) tests	1081:1126	EEVM was evaluated in methylthiazolyldiphenyl-tetrazolium bromide (MTT) (3, 10, 30, and 90 μg/ml) and neutrophils phagocytic activity (1, 3, and 10 μg/ml) tests.
34102271	11	73	theme	pleurisy	2021:2028	arg1	model					2030:2034	carrageenan-induced pleurisy model	2001:2034	carrageenan-induced pleurisy model	2001:2034	EEVM significantly inhibited leukocytes migration/proteins exudation in carrageenan-induced pleurisy model.
34102271	5	74	dep	bromide	1025:1031	arg1	tests					1122:1126	phagocytic activity (1, 3, and 10 μg/ml) tests	1081:1126	phagocytic activity (1, 3, and 10 μg/ml) tests	1081:1126	EEVM was evaluated in methylthiazolyldiphenyl-tetrazolium bromide (MTT) (3, 10, 30, and 90 μg/ml) and neutrophils phagocytic activity (1, 3, and 10 μg/ml) tests.
34102271	2	75	theme	long-term	539:547	arg1	treatment					549:557	all long-term treatment	535:557	all long-term treatment with conventional medications lead to adverse effects	535:611	The rheumatological and rheumatic diseases affect 0.3-1.0% of the world population and all long-term treatment with conventional medications lead to adverse effects.
34102271	7	76	theme	-induced	1473:1480	arg1	pleurisy					1482:1489	Mycobacterium bovis (bacillus Calmette-Guerin - BCG)-induced pleurisy	1421:1489	Mycobacterium bovis (bacillus Calmette-Guerin - BCG)-induced pleurisy	1421:1489	The EEVM (30 and 100 mg/kg, p.o.) was tested against the Complete Freund Adjuvant (CFA)-induced paw persistent inflammation and Mycobacterium bovis (bacillus Calmette-Guerin - BCG)-induced pleurisy in C57bL6 mice.
34102271	10	77	theme	neutrophils	1879:1889	arg1	activity					1891:1898	phagocytic neutrophils activity	1868:1898	phagocytic neutrophils activity	1868:1898	EEVM did not present cytotoxicity in MTT assay, however EEVM reduced phagocytic neutrophils activity at all tested concentration.
34102271	8	78	theme	48.99 mg	1655:1662	arg1	/g					1669:1670	48.99 mg (TAE)/g	1655:1670	48.99 mg (TAE)/g in relation to tannins	1655:1693	RESULTS The chemical composition of EEVM showed 157.06 mg (GAE)/g in relation to phenolic compounds, 82.13 mg (RUE)/g in relation to flavonoids and 48.99 mg (TAE)/g in relation to tannins.
34102271	8	78	theme	48.99 mg	1655:1662	arg1	compounds					1597:1605	phenolic compounds	1588:1605	phenolic compounds	1588:1605	RESULTS The chemical composition of EEVM showed 157.06 mg (GAE)/g in relation to phenolic compounds, 82.13 mg (RUE)/g in relation to flavonoids and 48.99 mg (TAE)/g in relation to tannins.
34102271	11	79	theme	migration/proteins	1969:1986	arg1	exudation					1988:1996	leukocytes migration/proteins exudation	1958:1996	leukocytes migration/proteins exudation	1958:1996	EEVM significantly inhibited leukocytes migration/proteins exudation in carrageenan-induced pleurisy model.
34102271	0	80	from	properties	18:27	arg1	macrocarpa					64:73	Vatairea macrocarpa	55:73	Vatairea macrocarpa	55:73	Anti-inflammatory properties of ethanolic extract from Vatairea macrocarpa leaves.
34102271	5	81	theme	10 μg/ml	1112:1119	arg1	tests					1122:1126	phagocytic activity (1, 3, and 10 μg/ml) tests	1081:1126	phagocytic activity (1, 3, and 10 μg/ml) tests	1081:1126	EEVM was evaluated in methylthiazolyldiphenyl-tetrazolium bromide (MTT) (3, 10, 30, and 90 μg/ml) and neutrophils phagocytic activity (1, 3, and 10 μg/ml) tests.
34102271	12	82	theme	EEVM	2065:2068	arg1	administration					2047:2060	The daily administration	2037:2060	The daily administration of EEVM	2037:2068	The daily administration of EEVM inhibited the inflammatory parameters in BCG and CFA models.
34102271	8	83	theme	TAE	1665:1667	arg1	/g					1669:1670	48.99 mg (TAE)/g	1655:1670	48.99 mg (TAE)/g in relation to tannins	1655:1693	RESULTS The chemical composition of EEVM showed 157.06 mg (GAE)/g in relation to phenolic compounds, 82.13 mg (RUE)/g in relation to flavonoids and 48.99 mg (TAE)/g in relation to tannins.
34102271	8	83	theme	TAE	1665:1667	arg1	compounds					1597:1605	phenolic compounds	1588:1605	phenolic compounds	1588:1605	RESULTS The chemical composition of EEVM showed 157.06 mg (GAE)/g in relation to phenolic compounds, 82.13 mg (RUE)/g in relation to flavonoids and 48.99 mg (TAE)/g in relation to tannins.
34102271	1	84	theme	condition	323:331	arg1	treatment					306:314	the treatment	302:314	the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling	302:445	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	0	85	from	macrocarpa	64:73	arg1	properties					18:27	Anti-inflammatory properties	0:27	Anti-inflammatory properties of ethanolic extract from Vatairea macrocarpa	0:73	Anti-inflammatory properties of ethanolic extract from Vatairea macrocarpa leaves.
34102271	0	85	from	macrocarpa	64:73	arg1	extract					42:48	ethanolic extract	32:48	ethanolic extract from Vatairea macrocarpa	32:73	Anti-inflammatory properties of ethanolic extract from Vatairea macrocarpa leaves.
34102271	10	86	attach	present	1812:1818	arg1	assay					1840:1844	MTT assay	1836:1844	MTT assay	1836:1844	EEVM did not present cytotoxicity in MTT assay, however EEVM reduced phagocytic neutrophils activity at all tested concentration.
34102271	10	86	attach	present	1812:1818	arg2	EEVM					1799:1802	EEVM	1799:1802	EEVM	1799:1802	EEVM did not present cytotoxicity in MTT assay, however EEVM reduced phagocytic neutrophils activity at all tested concentration.
34102271	10	87	theme	tested	1907:1912	arg1	concentration					1914:1926	all tested concentration	1903:1926	all tested concentration	1903:1926	EEVM did not present cytotoxicity in MTT assay, however EEVM reduced phagocytic neutrophils activity at all tested concentration.
34102271	6	88	theme	Swiss	1281:1285	arg1	mice					1287:1290	Swiss mice	1281:1290	Swiss mice	1281:1290	For in vivo models, the EEVM (10, 30, 100, and 300 mg/kg) was orally administered (p.o.) for inflammatory evaluation in carrageenan-induced pleurisy in Swiss mice.
34102271	8	89	dep	RESULTS	1507:1513	arg1	composition					1528:1538	The chemical composition	1515:1538	RESULTS The chemical composition of EEVM	1507:1546	RESULTS The chemical composition of EEVM showed 157.06 mg (GAE)/g in relation to phenolic compounds, 82.13 mg (RUE)/g in relation to flavonoids and 48.99 mg (TAE)/g in relation to tannins.
34102271	1	90	theme	ETHNOPHARMACOLOGICAL	83:102	arg1	RELEVANCE					104:112	ETHNOPHARMACOLOGICAL RELEVANCE	83:112	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family)	83:255	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	1	90	theme	ETHNOPHARMACOLOGICAL	83:102	arg1	remedy					269:274	remedy	269:274	remedy after cold maceration	269:296	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	1	91	theme	Fabaceae	240:247	arg1	family					249:254	Fabaceae family	240:254	Fabaceae family	240:254	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	1	91	theme	Fabaceae	240:247	arg1	the					154:156	the	154:156	the	154:156	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	3	92	from	AIM	614:616	arg1	macrocarpa					745:754	V. macrocarpa	742:754	V. macrocarpa leaves (EEVM) in experimental models	742:791	AIM OF THE STUDY To investigate the chemical composition and the anti-inflammatory properties and of the ethanolic extract from V. macrocarpa leaves (EEVM) in experimental models.
34102271	3	92	from	AIM	614:616	arg1	EEVM					764:767	EEVM	764:767	EEVM	764:767	AIM OF THE STUDY To investigate the chemical composition and the anti-inflammatory properties and of the ethanolic extract from V. macrocarpa leaves (EEVM) in experimental models.
34102271	2	93	theme	population	520:529	arg1	%					505:505	0.3-1.0%	498:505	0.3-1.0% of the world population	498:529	The rheumatological and rheumatic diseases affect 0.3-1.0% of the world population and all long-term treatment with conventional medications lead to adverse effects.
34102271	2	93	theme	population	520:529	arg1	population					520:529	the world population	510:529	the world population	510:529	The rheumatological and rheumatic diseases affect 0.3-1.0% of the world population and all long-term treatment with conventional medications lead to adverse effects.
34102271	2	93	theme	population	520:529	arg1	treatment					549:557	all long-term treatment	535:557	all long-term treatment with conventional medications lead to adverse effects	535:611	The rheumatological and rheumatic diseases affect 0.3-1.0% of the world population and all long-term treatment with conventional medications lead to adverse effects.
34102271	0	94	theme	ethanolic	32:40	arg1	extract					42:48	ethanolic extract	32:48	ethanolic extract from Vatairea macrocarpa	32:73	Anti-inflammatory properties of ethanolic extract from Vatairea macrocarpa leaves.
34102271	1	95	theme	cold	282:285	arg1	maceration					287:296	cold maceration	282:296	cold maceration	282:296	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	1	96	theme	traditional	127:137	arg1	the					154:156	the	154:156	the	154:156	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	1	96	theme	traditional	127:137	arg1	heartwood					169:177	heartwood	169:177	heartwood from Vatairea macrocarpa (Benth)	169:210	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	1	96	theme	traditional	127:137	arg1	medicine					144:151	Brazilian traditional folk medicine	117:151	Brazilian traditional folk medicine	117:151	ETHNOPHARMACOLOGICAL RELEVANCE In Brazilian traditional folk medicine, the leaves and heartwood from Vatairea macrocarpa (Benth) Ducke (Angelim-of-Cerrado) (Fabaceae family) are used as remedy after cold maceration for the treatment of the condition popularly known as rheumatism, as well as for the general inflammatory aspects such as pains, injury and swelling.
34102271	5	97	theme	methylthiazolyldiphenyl-tetrazolium	989:1023	arg1	90 μg/ml					1055:1062	3, 10, 30, and 90 μg/ml	1040:1062	3, 10, 30, and 90 μg/ml	1040:1062	EEVM was evaluated in methylthiazolyldiphenyl-tetrazolium bromide (MTT) (3, 10, 30, and 90 μg/ml) and neutrophils phagocytic activity (1, 3, and 10 μg/ml) tests.
34102271	5	97	theme	methylthiazolyldiphenyl-tetrazolium	989:1023	arg1	MTT					1034:1036	MTT	1034:1036	MTT	1034:1036	EEVM was evaluated in methylthiazolyldiphenyl-tetrazolium bromide (MTT) (3, 10, 30, and 90 μg/ml) and neutrophils phagocytic activity (1, 3, and 10 μg/ml) tests.
34102271	5	97	theme	methylthiazolyldiphenyl-tetrazolium	989:1023	arg1	bromide					1025:1031	methylthiazolyldiphenyl-tetrazolium bromide	989:1031	methylthiazolyldiphenyl-tetrazolium bromide (MTT) (3, 10, 30, and 90 μg/ml)	989:1063	EEVM was evaluated in methylthiazolyldiphenyl-tetrazolium bromide (MTT) (3, 10, 30, and 90 μg/ml) and neutrophils phagocytic activity (1, 3, and 10 μg/ml) tests.
34102271	12	98	theme	BCG	2111:2113	arg1	models					2123:2128	BCG and CFA models	2111:2128	BCG and CFA models	2111:2128	The daily administration of EEVM inhibited the inflammatory parameters in BCG and CFA models.
34102271	12	99	theme	CFA	2119:2121	arg1	models					2123:2128	BCG and CFA models	2111:2128	BCG and CFA models	2111:2128	The daily administration of EEVM inhibited the inflammatory parameters in BCG and CFA models.
34102271	8	100	theme	157.06 mg	1555:1563	arg1	/g					1570:1571	157.06 mg (GAE)/g	1555:1571	157.06 mg (GAE)/g in relation to phenolic compounds, 82.13 mg (RUE)/g in relation to flavonoids and 48.99 mg (TAE)/g in relation to tannins	1555:1693	RESULTS The chemical composition of EEVM showed 157.06 mg (GAE)/g in relation to phenolic compounds, 82.13 mg (RUE)/g in relation to flavonoids and 48.99 mg (TAE)/g in relation to tannins.
34102271	13	101	theme	present	2147:2153	arg1	study					2155:2159	The present study	2143:2159	The present study	2143:2159	CONCLUSIONS The present study showed anti-inflammatory features of EEVM (V. macrocarpa) as a natural agent against inflammatory diseases.
34102271	9	102	theme	flavonoid	1700:1708	arg1	compounds					1710:1718	The flavonoid compounds	1696:1718	The flavonoid compounds identified	1696:1729	The flavonoid compounds identified were catechin, epicatechin and kaempferol-3-O-a-l-rhamnopyranoside.
34102271	9	102	theme	flavonoid	1700:1708	arg1	catechin					1736:1743	catechin	1736:1743	catechin	1736:1743	The flavonoid compounds identified were catechin, epicatechin and kaempferol-3-O-a-l-rhamnopyranoside.
34102271	8	103	theme	GAE	1566:1568	arg1	/g					1570:1571	157.06 mg (GAE)/g	1555:1571	157.06 mg (GAE)/g in relation to phenolic compounds, 82.13 mg (RUE)/g in relation to flavonoids and 48.99 mg (TAE)/g in relation to tannins	1555:1693	RESULTS The chemical composition of EEVM showed 157.06 mg (GAE)/g in relation to phenolic compounds, 82.13 mg (RUE)/g in relation to flavonoids and 48.99 mg (TAE)/g in relation to tannins.
34102271	7	104	dep	bovis	1435:1439	arg1	BCG					1469:1471	BCG	1469:1471	BCG	1469:1471	The EEVM (30 and 100 mg/kg, p.o.) was tested against the Complete Freund Adjuvant (CFA)-induced paw persistent inflammation and Mycobacterium bovis (bacillus Calmette-Guerin - BCG)-induced pleurisy in C57bL6 mice.
34102271	7	104	dep	bovis	1435:1439	arg1	Calmette-Guerin					1451:1465	bacillus Calmette-Guerin	1442:1465	bacillus Calmette-Guerin	1442:1465	The EEVM (30 and 100 mg/kg, p.o.) was tested against the Complete Freund Adjuvant (CFA)-induced paw persistent inflammation and Mycobacterium bovis (bacillus Calmette-Guerin - BCG)-induced pleurisy in C57bL6 mice.
34102271	7	105	theme	persistent	1393:1402	arg1	inflammation					1404:1415	the Complete Freund Adjuvant (CFA)-induced paw persistent inflammation	1346:1415	the Complete Freund Adjuvant (CFA)-induced paw persistent inflammation	1346:1415	The EEVM (30 and 100 mg/kg, p.o.) was tested against the Complete Freund Adjuvant (CFA)-induced paw persistent inflammation and Mycobacterium bovis (bacillus Calmette-Guerin - BCG)-induced pleurisy in C57bL6 mice.
34102271	2	106	with	treatment	549:557	arg1	medications					577:587	conventional medications	564:587	conventional medications lead to adverse effects	564:611	The rheumatological and rheumatic diseases affect 0.3-1.0% of the world population and all long-term treatment with conventional medications lead to adverse effects.
34502951	1	0	theme	organic	150:156	arg1	biomaterials					158:169	biodegradable and natural organic biomaterials	124:169	biodegradable and natural organic biomaterials	124:169	The novel use of ionic liquid as a solvent for biodegradable and natural organic biomaterials has increasingly sparked interest in the biomedical field.
34502951	4	1	theme	conformational	1034:1047	arg1	change					1049:1054	a wavering conformational change	1023:1054	a wavering conformational change	1023:1054	The change in the morphology of the biocomposites (by scanning electron microscope, SEM) and their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR) showed that the samples underwent a wavering conformational change on a microscopic level, resulting in strong interactions and changes in their crystalline structures such as the CA crystalline and silk beta-pleated sheets once the different ratios were applied.
34502951	7	2	theme	biomedical	1810:1819	arg1	applications					1821:1832	various biomedical applications	1802:1832	various biomedical applications	1802:1832	This study provides the basis for the comprehension of the protein-polysaccharide composites for various biomedical applications.
34502951	5	3	theme	scanning	1266:1273	arg1	DSC					1288:1290	DSC	1288:1290	DSC	1288:1290	Differential scanning calorimetry (DSC) results demonstrated that strong molecular interactions were generated between CA and silk chains, providing the blended films lower glass transitions than those of the pure silk or cellulose acetate.
34502951	5	3	theme	scanning	1266:1273	arg1	calorimetry					1275:1285	Differential scanning calorimetry	1253:1285	Differential scanning calorimetry (DSC) results	1253:1299	Differential scanning calorimetry (DSC) results demonstrated that strong molecular interactions were generated between CA and silk chains, providing the blended films lower glass transitions than those of the pure silk or cellulose acetate.
34502951	5	4	theme	lower	1420:1424	arg1	films					1414:1418	the blended films	1402:1418	the blended films lower glass transitions than those of the pure silk or cellulose acetate	1402:1491	Differential scanning calorimetry (DSC) results demonstrated that strong molecular interactions were generated between CA and silk chains, providing the blended films lower glass transitions than those of the pure silk or cellulose acetate.
34502951	4	5	theme	electron	873:880	arg1	microscope					882:891	scanning electron microscope	864:891	scanning electron microscope	864:891	The change in the morphology of the biocomposites (by scanning electron microscope, SEM) and their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR) showed that the samples underwent a wavering conformational change on a microscopic level, resulting in strong interactions and changes in their crystalline structures such as the CA crystalline and silk beta-pleated sheets once the different ratios were applied.
34502951	4	6	theme	different	1222:1230	arg1	ratios					1232:1237	the different ratios	1218:1237	the different ratios	1218:1237	The change in the morphology of the biocomposites (by scanning electron microscope, SEM) and their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR) showed that the samples underwent a wavering conformational change on a microscopic level, resulting in strong interactions and changes in their crystalline structures such as the CA crystalline and silk beta-pleated sheets once the different ratios were applied.
34502951	3	7	theme	silk	559:562	arg1	protein					572:578	silk fibroin protein	559:578	silk fibroin protein	559:578	Here, a blended system consisting of Bombyx Mori silk fibroin protein and a cellulose derivative, cellulose acetate (CA), in the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIMAc) was regenerated and underwent characterization to understand the structure and physical properties of the films.
34502951	4	8	theme	analysis	929:936	arg1	morphology					828:837	the morphology	824:837	the morphology of the biocomposites (by scanning electron microscope, SEM) and their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR)	824:987	The change in the morphology of the biocomposites (by scanning electron microscope, SEM) and their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR) showed that the samples underwent a wavering conformational change on a microscopic level, resulting in strong interactions and changes in their crystalline structures such as the CA crystalline and silk beta-pleated sheets once the different ratios were applied.
34502951	4	9	theme	scanning	864:871	arg1	microscope					882:891	scanning electron microscope	864:891	scanning electron microscope	864:891	The change in the morphology of the biocomposites (by scanning electron microscope, SEM) and their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR) showed that the samples underwent a wavering conformational change on a microscopic level, resulting in strong interactions and changes in their crystalline structures such as the CA crystalline and silk beta-pleated sheets once the different ratios were applied.
34502951	2	10	theme	proteins	369:376	arg1	capability					335:344	the capability	331:344	the capability of polysaccharides and proteins to dissolve seamlessly in ionic liquid and form fine and tunable biomaterials after regeneration	331:473	As compared to more volatile traditional solvents that rapidly degrade the protein molecular weight, the capability of polysaccharides and proteins to dissolve seamlessly in ionic liquid and form fine and tunable biomaterials after regeneration is the key interest of this study.
34502951	2	10	theme	proteins	369:376	arg1	interest					486:493	the key interest	478:493	the key interest of this study	478:507	As compared to more volatile traditional solvents that rapidly degrade the protein molecular weight, the capability of polysaccharides and proteins to dissolve seamlessly in ionic liquid and form fine and tunable biomaterials after regeneration is the key interest of this study.
34502951	3	11	dep	Bombyx	547:552	arg1	derivative					596:605	a cellulose derivative	584:605	a cellulose derivative	584:605	Here, a blended system consisting of Bombyx Mori silk fibroin protein and a cellulose derivative, cellulose acetate (CA), in the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIMAc) was regenerated and underwent characterization to understand the structure and physical properties of the films.
34502951	3	11	dep	Bombyx	547:552	arg1	acetate					618:624	cellulose acetate	608:624	cellulose acetate (CA)	608:629	Here, a blended system consisting of Bombyx Mori silk fibroin protein and a cellulose derivative, cellulose acetate (CA), in the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIMAc) was regenerated and underwent characterization to understand the structure and physical properties of the films.
34502951	3	11	dep	Bombyx	547:552	arg1	Mori					554:557	Bombyx Mori silk fibroin protein and a cellulose derivative, cellulose acetate (CA),	547:630	Bombyx Mori silk fibroin protein and a cellulose derivative, cellulose acetate (CA), in the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIMAc)	547:695	Here, a blended system consisting of Bombyx Mori silk fibroin protein and a cellulose derivative, cellulose acetate (CA), in the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIMAc) was regenerated and underwent characterization to understand the structure and physical properties of the films.
34502951	3	11	dep	Bombyx	547:552	arg1	protein					572:578	silk fibroin protein	559:578	silk fibroin protein	559:578	Here, a blended system consisting of Bombyx Mori silk fibroin protein and a cellulose derivative, cellulose acetate (CA), in the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIMAc) was regenerated and underwent characterization to understand the structure and physical properties of the films.
34502951	4	12	theme	Fourier-transform	942:958	arg1	spectroscopy					969:980	Fourier-transform infrared spectroscopy	942:980	Fourier-transform infrared spectroscopy	942:980	The change in the morphology of the biocomposites (by scanning electron microscope, SEM) and their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR) showed that the samples underwent a wavering conformational change on a microscopic level, resulting in strong interactions and changes in their crystalline structures such as the CA crystalline and silk beta-pleated sheets once the different ratios were applied.
34502951	5	13	theme	pure	1462:1465	arg1	silk					1467:1470	the pure silk	1458:1470	the pure silk	1458:1470	Differential scanning calorimetry (DSC) results demonstrated that strong molecular interactions were generated between CA and silk chains, providing the blended films lower glass transitions than those of the pure silk or cellulose acetate.
34502951	5	14	theme	Differential	1253:1264	arg1	DSC					1288:1290	DSC	1288:1290	DSC	1288:1290	Differential scanning calorimetry (DSC) results demonstrated that strong molecular interactions were generated between CA and silk chains, providing the blended films lower glass transitions than those of the pure silk or cellulose acetate.
34502951	5	14	theme	Differential	1253:1264	arg1	calorimetry					1275:1285	Differential scanning calorimetry	1253:1285	Differential scanning calorimetry (DSC) results	1253:1299	Differential scanning calorimetry (DSC) results demonstrated that strong molecular interactions were generated between CA and silk chains, providing the blended films lower glass transitions than those of the pure silk or cellulose acetate.
34502951	1	15	theme	novel	81:85	arg1	use					87:89	The novel use	77:89	The novel use of ionic liquid as a solvent for biodegradable and natural organic biomaterials	77:169	The novel use of ionic liquid as a solvent for biodegradable and natural organic biomaterials has increasingly sparked interest in the biomedical field.
34502951	7	16	theme	protein-polysaccharide	1764:1785	arg1	composites					1787:1796	the protein-polysaccharide composites	1760:1796	the protein-polysaccharide composites	1760:1796	This study provides the basis for the comprehension of the protein-polysaccharide composites for various biomedical applications.
34502951	4	17	theme	crystalline	1134:1144	arg1	crystalline					1172:1182	the CA crystalline and silk beta-pleated sheets	1165:1211	crystalline	1172:1182	The change in the morphology of the biocomposites (by scanning electron microscope, SEM) and their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR) showed that the samples underwent a wavering conformational change on a microscopic level, resulting in strong interactions and changes in their crystalline structures such as the CA crystalline and silk beta-pleated sheets once the different ratios were applied.
34502951	4	17	theme	crystalline	1134:1144	arg1	structures					1146:1155	their crystalline structures	1128:1155	their crystalline structures such as the CA crystalline and silk beta-pleated sheets once the different ratios	1128:1237	The change in the morphology of the biocomposites (by scanning electron microscope, SEM) and their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR) showed that the samples underwent a wavering conformational change on a microscopic level, resulting in strong interactions and changes in their crystalline structures such as the CA crystalline and silk beta-pleated sheets once the different ratios were applied.
34502951	4	17	theme	crystalline	1134:1144	arg1	sheets					1206:1211	the CA crystalline and silk beta-pleated sheets	1165:1211	sheets	1206:1211	The change in the morphology of the biocomposites (by scanning electron microscope, SEM) and their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR) showed that the samples underwent a wavering conformational change on a microscopic level, resulting in strong interactions and changes in their crystalline structures such as the CA crystalline and silk beta-pleated sheets once the different ratios were applied.
34502951	0	18	theme	Acetate	15:21	arg1	Materials					36:44	Silk-Cellulose Acetate Biocomposite Materials	0:44	Silk-Cellulose Acetate Biocomposite Materials	0:44	Silk-Cellulose Acetate Biocomposite Materials Regenerated from Ionic Liquid.
34502951	3	19	theme	blended	518:524	arg1	system					526:531	a blended system	516:531	a blended system consisting of Bombyx Mori silk fibroin protein and a cellulose derivative, cellulose acetate (CA), in the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIMAc)	516:695	Here, a blended system consisting of Bombyx Mori silk fibroin protein and a cellulose derivative, cellulose acetate (CA), in the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIMAc) was regenerated and underwent characterization to understand the structure and physical properties of the films.
34502951	4	20	theme	biocomposites	846:858	arg1	morphology					828:837	the morphology	824:837	the morphology of the biocomposites (by scanning electron microscope, SEM) and their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR)	824:987	The change in the morphology of the biocomposites (by scanning electron microscope, SEM) and their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR) showed that the samples underwent a wavering conformational change on a microscopic level, resulting in strong interactions and changes in their crystalline structures such as the CA crystalline and silk beta-pleated sheets once the different ratios were applied.
34502951	4	21	theme	beta-pleated	1193:1204	arg1	sheets					1206:1211	the CA crystalline and silk beta-pleated sheets	1165:1211	sheets	1206:1211	The change in the morphology of the biocomposites (by scanning electron microscope, SEM) and their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR) showed that the samples underwent a wavering conformational change on a microscopic level, resulting in strong interactions and changes in their crystalline structures such as the CA crystalline and silk beta-pleated sheets once the different ratios were applied.
34502951	3	22	theme	fibroin	564:570	arg1	protein					572:578	silk fibroin protein	559:578	silk fibroin protein	559:578	Here, a blended system consisting of Bombyx Mori silk fibroin protein and a cellulose derivative, cellulose acetate (CA), in the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIMAc) was regenerated and underwent characterization to understand the structure and physical properties of the films.
34502951	6	23	theme	higher	1526:1531	arg1	stability					1541:1549	higher thermal stability	1526:1549	higher thermal stability	1526:1549	All films that were blended had higher thermal stability than the pure cellulose acetate sample but presented gradual changes amongst the changing of ratios, as demonstrated by thermogravimetric analysis (TGA).
34502951	0	24	theme	Silk-Cellulose	0:13	arg1	Materials					36:44	Silk-Cellulose Acetate Biocomposite Materials	0:44	Silk-Cellulose Acetate Biocomposite Materials	0:44	Silk-Cellulose Acetate Biocomposite Materials Regenerated from Ionic Liquid.
34502951	5	25	theme	cellulose	1475:1483	arg1	acetate					1485:1491	cellulose acetate	1475:1491	cellulose acetate	1475:1491	Differential scanning calorimetry (DSC) results demonstrated that strong molecular interactions were generated between CA and silk chains, providing the blended films lower glass transitions than those of the pure silk or cellulose acetate.
34502951	5	26	theme	glass	1426:1430	arg1	transitions					1432:1442	glass transitions	1426:1442	glass transitions	1426:1442	Differential scanning calorimetry (DSC) results demonstrated that strong molecular interactions were generated between CA and silk chains, providing the blended films lower glass transitions than those of the pure silk or cellulose acetate.
34502951	4	27	theme	strong	1093:1098	arg1	interactions					1100:1111	strong interactions	1093:1111	strong interactions	1093:1111	The change in the morphology of the biocomposites (by scanning electron microscope, SEM) and their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR) showed that the samples underwent a wavering conformational change on a microscopic level, resulting in strong interactions and changes in their crystalline structures such as the CA crystalline and silk beta-pleated sheets once the different ratios were applied.
34502951	4	28	from	changes	1117:1123	arg1	crystalline					1172:1182	the CA crystalline and silk beta-pleated sheets	1165:1211	crystalline	1172:1182	The change in the morphology of the biocomposites (by scanning electron microscope, SEM) and their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR) showed that the samples underwent a wavering conformational change on a microscopic level, resulting in strong interactions and changes in their crystalline structures such as the CA crystalline and silk beta-pleated sheets once the different ratios were applied.
34502951	4	28	from	changes	1117:1123	arg1	structures					1146:1155	their crystalline structures	1128:1155	their crystalline structures such as the CA crystalline and silk beta-pleated sheets once the different ratios	1128:1237	The change in the morphology of the biocomposites (by scanning electron microscope, SEM) and their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR) showed that the samples underwent a wavering conformational change on a microscopic level, resulting in strong interactions and changes in their crystalline structures such as the CA crystalline and silk beta-pleated sheets once the different ratios were applied.
34502951	4	28	from	changes	1117:1123	arg1	sheets					1206:1211	the CA crystalline and silk beta-pleated sheets	1165:1211	sheets	1206:1211	The change in the morphology of the biocomposites (by scanning electron microscope, SEM) and their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR) showed that the samples underwent a wavering conformational change on a microscopic level, resulting in strong interactions and changes in their crystalline structures such as the CA crystalline and silk beta-pleated sheets once the different ratios were applied.
34502951	4	29	theme	wavering	1025:1032	arg1	change					1049:1054	a wavering conformational change	1023:1054	a wavering conformational change	1023:1054	The change in the morphology of the biocomposites (by scanning electron microscope, SEM) and their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR) showed that the samples underwent a wavering conformational change on a microscopic level, resulting in strong interactions and changes in their crystalline structures such as the CA crystalline and silk beta-pleated sheets once the different ratios were applied.
34502951	7	30	theme	various	1802:1808	arg1	applications					1821:1832	various biomedical applications	1802:1832	various biomedical applications	1802:1832	This study provides the basis for the comprehension of the protein-polysaccharide composites for various biomedical applications.
34502951	2	31	theme	molecular	313:321	arg1	weight					323:328	the protein molecular weight	301:328	the protein molecular weight	301:328	As compared to more volatile traditional solvents that rapidly degrade the protein molecular weight, the capability of polysaccharides and proteins to dissolve seamlessly in ionic liquid and form fine and tunable biomaterials after regeneration is the key interest of this study.
34502951	2	32	theme	tunable	435:441	arg1	biomaterials					443:454	fine and tunable biomaterials	426:454	fine and tunable biomaterials	426:454	As compared to more volatile traditional solvents that rapidly degrade the protein molecular weight, the capability of polysaccharides and proteins to dissolve seamlessly in ionic liquid and form fine and tunable biomaterials after regeneration is the key interest of this study.
34502951	4	33	from	change	814:819	arg1	morphology					828:837	the morphology	824:837	the morphology of the biocomposites (by scanning electron microscope, SEM) and their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR)	824:987	The change in the morphology of the biocomposites (by scanning electron microscope, SEM) and their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR) showed that the samples underwent a wavering conformational change on a microscopic level, resulting in strong interactions and changes in their crystalline structures such as the CA crystalline and silk beta-pleated sheets once the different ratios were applied.
34502951	2	34	theme	protein	305:311	arg1	weight					323:328	the protein molecular weight	301:328	the protein molecular weight	301:328	As compared to more volatile traditional solvents that rapidly degrade the protein molecular weight, the capability of polysaccharides and proteins to dissolve seamlessly in ionic liquid and form fine and tunable biomaterials after regeneration is the key interest of this study.
34502951	5	35	theme	calorimetry	1275:1285	arg1	results					1293:1299	Differential scanning calorimetry (DSC) results	1253:1299	Differential scanning calorimetry (DSC) results	1253:1299	Differential scanning calorimetry (DSC) results demonstrated that strong molecular interactions were generated between CA and silk chains, providing the blended films lower glass transitions than those of the pure silk or cellulose acetate.
34502951	2	36	theme	fine	426:429	arg1	biomaterials					443:454	fine and tunable biomaterials	426:454	fine and tunable biomaterials	426:454	As compared to more volatile traditional solvents that rapidly degrade the protein molecular weight, the capability of polysaccharides and proteins to dissolve seamlessly in ionic liquid and form fine and tunable biomaterials after regeneration is the key interest of this study.
34502951	4	37	theme	structure	919:927	arg1	analysis					929:936	their secondary structure analysis	903:936	their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR)	903:987	The change in the morphology of the biocomposites (by scanning electron microscope, SEM) and their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR) showed that the samples underwent a wavering conformational change on a microscopic level, resulting in strong interactions and changes in their crystalline structures such as the CA crystalline and silk beta-pleated sheets once the different ratios were applied.
34502951	4	37	theme	structure	919:927	arg1	FTIR					983:986	FTIR	983:986	FTIR	983:986	The change in the morphology of the biocomposites (by scanning electron microscope, SEM) and their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR) showed that the samples underwent a wavering conformational change on a microscopic level, resulting in strong interactions and changes in their crystalline structures such as the CA crystalline and silk beta-pleated sheets once the different ratios were applied.
34502951	3	38	theme	cellulose	586:594	arg1	derivative					596:605	a cellulose derivative	584:605	a cellulose derivative	584:605	Here, a blended system consisting of Bombyx Mori silk fibroin protein and a cellulose derivative, cellulose acetate (CA), in the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIMAc) was regenerated and underwent characterization to understand the structure and physical properties of the films.
34502951	3	38	theme	cellulose	586:594	arg1	acetate					618:624	cellulose acetate	608:624	cellulose acetate (CA)	608:629	Here, a blended system consisting of Bombyx Mori silk fibroin protein and a cellulose derivative, cellulose acetate (CA), in the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIMAc) was regenerated and underwent characterization to understand the structure and physical properties of the films.
34502951	5	39	theme	molecular	1326:1334	arg1	interactions					1336:1347	strong molecular interactions	1319:1347	strong molecular interactions	1319:1347	Differential scanning calorimetry (DSC) results demonstrated that strong molecular interactions were generated between CA and silk chains, providing the blended films lower glass transitions than those of the pure silk or cellulose acetate.
34502951	1	40	theme	biomedical	212:221	arg1	field					223:227	the biomedical field	208:227	the biomedical field	208:227	The novel use of ionic liquid as a solvent for biodegradable and natural organic biomaterials has increasingly sparked interest in the biomedical field.
34502951	2	41	theme	key	482:484	arg1	capability					335:344	the capability	331:344	the capability of polysaccharides and proteins to dissolve seamlessly in ionic liquid and form fine and tunable biomaterials after regeneration	331:473	As compared to more volatile traditional solvents that rapidly degrade the protein molecular weight, the capability of polysaccharides and proteins to dissolve seamlessly in ionic liquid and form fine and tunable biomaterials after regeneration is the key interest of this study.
34502951	2	41	theme	key	482:484	arg1	interest					486:493	the key interest	478:493	the key interest of this study	478:507	As compared to more volatile traditional solvents that rapidly degrade the protein molecular weight, the capability of polysaccharides and proteins to dissolve seamlessly in ionic liquid and form fine and tunable biomaterials after regeneration is the key interest of this study.
34502951	3	42	theme	physical	776:783	arg1	properties					785:794	physical properties	776:794	physical properties	776:794	Here, a blended system consisting of Bombyx Mori silk fibroin protein and a cellulose derivative, cellulose acetate (CA), in the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIMAc) was regenerated and underwent characterization to understand the structure and physical properties of the films.
34502951	4	43	theme	secondary	909:917	arg1	analysis					929:936	their secondary structure analysis	903:936	their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR)	903:987	The change in the morphology of the biocomposites (by scanning electron microscope, SEM) and their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR) showed that the samples underwent a wavering conformational change on a microscopic level, resulting in strong interactions and changes in their crystalline structures such as the CA crystalline and silk beta-pleated sheets once the different ratios were applied.
34502951	4	43	theme	secondary	909:917	arg1	FTIR					983:986	FTIR	983:986	FTIR	983:986	The change in the morphology of the biocomposites (by scanning electron microscope, SEM) and their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR) showed that the samples underwent a wavering conformational change on a microscopic level, resulting in strong interactions and changes in their crystalline structures such as the CA crystalline and silk beta-pleated sheets once the different ratios were applied.
34502951	5	44	theme	strong	1319:1324	arg1	interactions					1336:1347	strong molecular interactions	1319:1347	strong molecular interactions	1319:1347	Differential scanning calorimetry (DSC) results demonstrated that strong molecular interactions were generated between CA and silk chains, providing the blended films lower glass transitions than those of the pure silk or cellulose acetate.
34502951	6	45	theme	pure	1560:1563	arg1	sample					1583:1588	the pure cellulose acetate sample	1556:1588	the pure cellulose acetate sample	1556:1588	All films that were blended had higher thermal stability than the pure cellulose acetate sample but presented gradual changes amongst the changing of ratios, as demonstrated by thermogravimetric analysis (TGA).
34502951	6	46	theme	gradual	1604:1610	arg1	changes					1612:1618	gradual changes	1604:1618	gradual changes	1604:1618	All films that were blended had higher thermal stability than the pure cellulose acetate sample but presented gradual changes amongst the changing of ratios, as demonstrated by thermogravimetric analysis (TGA).
34502951	4	47	theme	infrared	960:967	arg1	spectroscopy					969:980	Fourier-transform infrared spectroscopy	942:980	Fourier-transform infrared spectroscopy	942:980	The change in the morphology of the biocomposites (by scanning electron microscope, SEM) and their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR) showed that the samples underwent a wavering conformational change on a microscopic level, resulting in strong interactions and changes in their crystalline structures such as the CA crystalline and silk beta-pleated sheets once the different ratios were applied.
34502951	4	48	theme	CA	1169:1170	arg1	crystalline					1172:1182	the CA crystalline and silk beta-pleated sheets	1165:1211	crystalline	1172:1182	The change in the morphology of the biocomposites (by scanning electron microscope, SEM) and their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR) showed that the samples underwent a wavering conformational change on a microscopic level, resulting in strong interactions and changes in their crystalline structures such as the CA crystalline and silk beta-pleated sheets once the different ratios were applied.
34502951	2	49	theme	polysaccharides	349:363	arg1	capability					335:344	the capability	331:344	the capability of polysaccharides and proteins to dissolve seamlessly in ionic liquid and form fine and tunable biomaterials after regeneration	331:473	As compared to more volatile traditional solvents that rapidly degrade the protein molecular weight, the capability of polysaccharides and proteins to dissolve seamlessly in ionic liquid and form fine and tunable biomaterials after regeneration is the key interest of this study.
34502951	2	49	theme	polysaccharides	349:363	arg1	interest					486:493	the key interest	478:493	the key interest of this study	478:507	As compared to more volatile traditional solvents that rapidly degrade the protein molecular weight, the capability of polysaccharides and proteins to dissolve seamlessly in ionic liquid and form fine and tunable biomaterials after regeneration is the key interest of this study.
34502951	5	50	dep	lower	1420:1424	arg1	transitions					1432:1442	glass transitions	1426:1442	glass transitions	1426:1442	Differential scanning calorimetry (DSC) results demonstrated that strong molecular interactions were generated between CA and silk chains, providing the blended films lower glass transitions than those of the pure silk or cellulose acetate.
34502951	7	51	theme	composites	1787:1796	arg1	comprehension					1743:1755	the comprehension	1739:1755	the comprehension of the protein-polysaccharide composites for various biomedical applications	1739:1832	This study provides the basis for the comprehension of the protein-polysaccharide composites for various biomedical applications.
34502951	3	52	theme	liquid	645:650	arg1	EMIMAc					689:694	EMIMAc	689:694	EMIMAc	689:694	Here, a blended system consisting of Bombyx Mori silk fibroin protein and a cellulose derivative, cellulose acetate (CA), in the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIMAc) was regenerated and underwent characterization to understand the structure and physical properties of the films.
34502951	3	52	theme	liquid	645:650	arg1	acetate					680:686	the ionic liquid 1-ethyl-3-methylimidazolium acetate	635:686	the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIMAc)	635:695	Here, a blended system consisting of Bombyx Mori silk fibroin protein and a cellulose derivative, cellulose acetate (CA), in the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIMAc) was regenerated and underwent characterization to understand the structure and physical properties of the films.
34502951	6	53	theme	thermogravimetric	1671:1687	arg1	TGA					1699:1701	TGA	1699:1701	TGA	1699:1701	All films that were blended had higher thermal stability than the pure cellulose acetate sample but presented gradual changes amongst the changing of ratios, as demonstrated by thermogravimetric analysis (TGA).
34502951	6	53	theme	thermogravimetric	1671:1687	arg1	analysis					1689:1696	thermogravimetric analysis	1671:1696	thermogravimetric analysis (TGA)	1671:1702	All films that were blended had higher thermal stability than the pure cellulose acetate sample but presented gradual changes amongst the changing of ratios, as demonstrated by thermogravimetric analysis (TGA).
34502951	4	54	theme	silk	1188:1191	arg1	sheets					1206:1211	the CA crystalline and silk beta-pleated sheets	1165:1211	sheets	1206:1211	The change in the morphology of the biocomposites (by scanning electron microscope, SEM) and their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR) showed that the samples underwent a wavering conformational change on a microscopic level, resulting in strong interactions and changes in their crystalline structures such as the CA crystalline and silk beta-pleated sheets once the different ratios were applied.
34502951	3	55	theme	cellulose	608:616	arg1	CA					627:628	CA	627:628	CA	627:628	Here, a blended system consisting of Bombyx Mori silk fibroin protein and a cellulose derivative, cellulose acetate (CA), in the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIMAc) was regenerated and underwent characterization to understand the structure and physical properties of the films.
34502951	3	55	theme	cellulose	608:616	arg1	derivative					596:605	a cellulose derivative	584:605	a cellulose derivative	584:605	Here, a blended system consisting of Bombyx Mori silk fibroin protein and a cellulose derivative, cellulose acetate (CA), in the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIMAc) was regenerated and underwent characterization to understand the structure and physical properties of the films.
34502951	3	55	theme	cellulose	608:616	arg1	acetate					618:624	cellulose acetate	608:624	cellulose acetate (CA)	608:629	Here, a blended system consisting of Bombyx Mori silk fibroin protein and a cellulose derivative, cellulose acetate (CA), in the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIMAc) was regenerated and underwent characterization to understand the structure and physical properties of the films.
34502951	3	56	theme	1-ethyl-3-methylimidazolium	652:678	arg1	EMIMAc					689:694	EMIMAc	689:694	EMIMAc	689:694	Here, a blended system consisting of Bombyx Mori silk fibroin protein and a cellulose derivative, cellulose acetate (CA), in the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIMAc) was regenerated and underwent characterization to understand the structure and physical properties of the films.
34502951	3	56	theme	1-ethyl-3-methylimidazolium	652:678	arg1	acetate					680:686	the ionic liquid 1-ethyl-3-methylimidazolium acetate	635:686	the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIMAc)	635:695	Here, a blended system consisting of Bombyx Mori silk fibroin protein and a cellulose derivative, cellulose acetate (CA), in the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIMAc) was regenerated and underwent characterization to understand the structure and physical properties of the films.
34502951	5	57	theme	blended	1406:1412	arg1	films					1414:1418	the blended films	1402:1418	the blended films lower glass transitions than those of the pure silk or cellulose acetate	1402:1491	Differential scanning calorimetry (DSC) results demonstrated that strong molecular interactions were generated between CA and silk chains, providing the blended films lower glass transitions than those of the pure silk or cellulose acetate.
34502951	1	58	theme	liquid	100:105	arg1	use					87:89	The novel use	77:89	The novel use of ionic liquid as a solvent for biodegradable and natural organic biomaterials	77:169	The novel use of ionic liquid as a solvent for biodegradable and natural organic biomaterials has increasingly sparked interest in the biomedical field.
34502951	3	59	theme	films	803:807	arg1	structure					762:770	the structure	758:770	the structure	758:770	Here, a blended system consisting of Bombyx Mori silk fibroin protein and a cellulose derivative, cellulose acetate (CA), in the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIMAc) was regenerated and underwent characterization to understand the structure and physical properties of the films.
34502951	3	59	theme	films	803:807	arg1	properties					785:794	physical properties	776:794	physical properties	776:794	Here, a blended system consisting of Bombyx Mori silk fibroin protein and a cellulose derivative, cellulose acetate (CA), in the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIMAc) was regenerated and underwent characterization to understand the structure and physical properties of the films.
34502951	2	60	theme	traditional	259:269	arg1	solvents					271:278	more volatile traditional solvents	245:278	more volatile traditional solvents that rapidly degrade the protein molecular weight	245:328	As compared to more volatile traditional solvents that rapidly degrade the protein molecular weight, the capability of polysaccharides and proteins to dissolve seamlessly in ionic liquid and form fine and tunable biomaterials after regeneration is the key interest of this study.
34502951	6	61	theme	acetate	1575:1581	arg1	sample					1583:1588	the pure cellulose acetate sample	1556:1588	the pure cellulose acetate sample	1556:1588	All films that were blended had higher thermal stability than the pure cellulose acetate sample but presented gradual changes amongst the changing of ratios, as demonstrated by thermogravimetric analysis (TGA).
34502951	1	62	theme	biodegradable	124:136	arg1	biomaterials					158:169	biodegradable and natural organic biomaterials	124:169	biodegradable and natural organic biomaterials	124:169	The novel use of ionic liquid as a solvent for biodegradable and natural organic biomaterials has increasingly sparked interest in the biomedical field.
34502951	6	63	theme	thermal	1533:1539	arg1	stability					1541:1549	higher thermal stability	1526:1549	higher thermal stability	1526:1549	All films that were blended had higher thermal stability than the pure cellulose acetate sample but presented gradual changes amongst the changing of ratios, as demonstrated by thermogravimetric analysis (TGA).
34502951	6	64	contain	had	1522:1524	arg2	stability					1541:1549	higher thermal stability	1526:1549	higher thermal stability	1526:1549	All films that were blended had higher thermal stability than the pure cellulose acetate sample but presented gradual changes amongst the changing of ratios, as demonstrated by thermogravimetric analysis (TGA).
34502951	6	64	contain	had	1522:1524	arg1	films					1498:1502	All films	1494:1502	All films that were blended	1494:1520	All films that were blended had higher thermal stability than the pure cellulose acetate sample but presented gradual changes amongst the changing of ratios, as demonstrated by thermogravimetric analysis (TGA).
34502951	3	65	theme	ionic	639:643	arg1	EMIMAc					689:694	EMIMAc	689:694	EMIMAc	689:694	Here, a blended system consisting of Bombyx Mori silk fibroin protein and a cellulose derivative, cellulose acetate (CA), in the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIMAc) was regenerated and underwent characterization to understand the structure and physical properties of the films.
34502951	3	65	theme	ionic	639:643	arg1	acetate					680:686	the ionic liquid 1-ethyl-3-methylimidazolium acetate	635:686	the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIMAc)	635:695	Here, a blended system consisting of Bombyx Mori silk fibroin protein and a cellulose derivative, cellulose acetate (CA), in the ionic liquid 1-ethyl-3-methylimidazolium acetate (EMIMAc) was regenerated and underwent characterization to understand the structure and physical properties of the films.
34502951	2	66	theme	volatile	250:257	arg1	solvents					271:278	more volatile traditional solvents	245:278	more volatile traditional solvents that rapidly degrade the protein molecular weight	245:328	As compared to more volatile traditional solvents that rapidly degrade the protein molecular weight, the capability of polysaccharides and proteins to dissolve seamlessly in ionic liquid and form fine and tunable biomaterials after regeneration is the key interest of this study.
34502951	6	67	theme	cellulose	1565:1573	arg1	sample					1583:1588	the pure cellulose acetate sample	1556:1588	the pure cellulose acetate sample	1556:1588	All films that were blended had higher thermal stability than the pure cellulose acetate sample but presented gradual changes amongst the changing of ratios, as demonstrated by thermogravimetric analysis (TGA).
34502951	4	68	theme	microscopic	1061:1071	arg1	level					1073:1077	a microscopic level	1059:1077	a microscopic level	1059:1077	The change in the morphology of the biocomposites (by scanning electron microscope, SEM) and their secondary structure analysis (by Fourier-transform infrared spectroscopy, FTIR) showed that the samples underwent a wavering conformational change on a microscopic level, resulting in strong interactions and changes in their crystalline structures such as the CA crystalline and silk beta-pleated sheets once the different ratios were applied.
34502951	2	69	theme	study	503:507	arg1	capability					335:344	the capability	331:344	the capability of polysaccharides and proteins to dissolve seamlessly in ionic liquid and form fine and tunable biomaterials after regeneration	331:473	As compared to more volatile traditional solvents that rapidly degrade the protein molecular weight, the capability of polysaccharides and proteins to dissolve seamlessly in ionic liquid and form fine and tunable biomaterials after regeneration is the key interest of this study.
34502951	2	69	theme	study	503:507	arg1	interest					486:493	the key interest	478:493	the key interest of this study	478:507	As compared to more volatile traditional solvents that rapidly degrade the protein molecular weight, the capability of polysaccharides and proteins to dissolve seamlessly in ionic liquid and form fine and tunable biomaterials after regeneration is the key interest of this study.
34502951	0	70	theme	Biocomposite	23:34	arg1	Materials					36:44	Silk-Cellulose Acetate Biocomposite Materials	0:44	Silk-Cellulose Acetate Biocomposite Materials	0:44	Silk-Cellulose Acetate Biocomposite Materials Regenerated from Ionic Liquid.
34502951	5	71	theme	silk	1379:1382	arg1	chains					1384:1389	silk chains	1379:1389	silk chains	1379:1389	Differential scanning calorimetry (DSC) results demonstrated that strong molecular interactions were generated between CA and silk chains, providing the blended films lower glass transitions than those of the pure silk or cellulose acetate.
34502951	1	72	theme	natural	142:148	arg1	biomaterials					158:169	biodegradable and natural organic biomaterials	124:169	biodegradable and natural organic biomaterials	124:169	The novel use of ionic liquid as a solvent for biodegradable and natural organic biomaterials has increasingly sparked interest in the biomedical field.
34298952	4	0	theme	structures	880:889	arg1	understanding					909:921	understanding	909:921	understanding	909:921	Among immunomodulatory glycans, Lewis type antigens have been of interest for at least two decades, while the importance of T/Tn antigens and related structures is still far from understanding.
34298952	4	0	theme	structures	880:889	arg1	importance					840:849	the importance	836:849	the importance of T/Tn antigens and related structures	836:889	Among immunomodulatory glycans, Lewis type antigens have been of interest for at least two decades, while the importance of T/Tn antigens and related structures is still far from understanding.
34298952	5	1	with	glycoepitopes	1000:1012	arg1	Gal					1039:1041	Gal	1039:1041	Gal	1039:1041	In the current work, we applied two plant lectins capable of distinguishing glycoepitopes with terminal GalNAc and Gal to identify glycoproteins that are their efficient carriers.
34298952	5	1	with	glycoepitopes	1000:1012	arg1	GalNAc					1028:1033	terminal GalNAc	1019:1033	terminal GalNAc	1019:1033	In the current work, we applied two plant lectins capable of distinguishing glycoepitopes with terminal GalNAc and Gal to identify glycoproteins that are their efficient carriers.
34298952	3	2	theme	maternal	704:711	arg1	response					720:727	the maternal immune response	700:727	the maternal immune response	700:727	Such interaction may be involved in modulation of the maternal immune response.
34298952	7	3	theme	inducible	1455:1463	arg1	protein					1465:1471	prolactin inducible protein	1445:1471	prolactin inducible protein	1445:1471	Net-O-glycosylation analysis results indicated that the latter three may actually carry T and/or Tn antigens, while in the case of prolactin inducible protein and lactotransferrin LacdiNAc and lactosamine glycoepitopes were more probable.
34298952	8	4	from	glycoproteins	1606:1618	arg1	network					1633:1639	the close network	1623:1639	the close network	1623:1639	STRING bioinformatics analysis linked the identified glycoproteins in the close network, indicating their involvement in immune (partially innate) processes.
34298952	1	5	theme	healthy	456:462	arg1	pregnancy					464:472	healthy pregnancy	456:472	healthy pregnancy	456:472	In light of recent research, there is increasing evidence showing that extracellular semen components have a significant impact on the immune reaction of the female partner, leading to the tolerogenic response enabling the embryo development and implantation as well as further progress of healthy pregnancy.
34298952	4	6	theme	T/Tn	854:857	arg1	antigens					859:866	T/Tn antigens	854:866	T/Tn antigens	854:866	Among immunomodulatory glycans, Lewis type antigens have been of interest for at least two decades, while the importance of T/Tn antigens and related structures is still far from understanding.
34298952	1	7	theme	research	185:192	arg1	light					169:173	light	169:173	light of recent research	169:192	In light of recent research, there is increasing evidence showing that extracellular semen components have a significant impact on the immune reaction of the female partner, leading to the tolerogenic response enabling the embryo development and implantation as well as further progress of healthy pregnancy.
34298952	7	8	theme	lactotransferrin	1477:1492	arg1	LacdiNAc					1494:1501	lactotransferrin LacdiNAc	1477:1501	lactotransferrin LacdiNAc	1477:1501	Net-O-glycosylation analysis results indicated that the latter three may actually carry T and/or Tn antigens, while in the case of prolactin inducible protein and lactotransferrin LacdiNAc and lactosamine glycoepitopes were more probable.
34298952	2	9	theme	cells	643:647	arg1	surface					625:631	the surface	621:631	the surface of immune cells	621:647	Seminal plasma glycoproteins are rich in the unique immunomodulatory glycoepitopes that may serve as ligands for endogenous lectins that decorate the surface of immune cells.
34298952	9	10	theme	plasma	1760:1765	arg1	ligands					1767:1773	potential seminal plasma ligands	1742:1773	potential seminal plasma ligands for endogenous Gal/GalNAc specific lectins with a possible role in modulation of maternal immune response during fertilization	1742:1900	Overall, our research revealed potential seminal plasma ligands for endogenous Gal/GalNAc specific lectins with a possible role in modulation of maternal immune response during fertilization.
34298952	1	11	theme	embryo	389:394	arg1	development					396:406	the embryo development	385:406	the embryo development	385:406	In light of recent research, there is increasing evidence showing that extracellular semen components have a significant impact on the immune reaction of the female partner, leading to the tolerogenic response enabling the embryo development and implantation as well as further progress of healthy pregnancy.
34298952	1	12	theme	increasing	204:213	arg1	evidence					215:222	increasing evidence	204:222	increasing evidence showing that extracellular semen components have a significant impact on the immune reaction of the female partner, leading to the tolerogenic response enabling the embryo development and implantation as well as further progress of healthy pregnancy	204:472	In light of recent research, there is increasing evidence showing that extracellular semen components have a significant impact on the immune reaction of the female partner, leading to the tolerogenic response enabling the embryo development and implantation as well as further progress of healthy pregnancy.
34298952	0	13	theme	Fertilization	143:155	arg1	Process					157:163	the Fertilization Process	139:163	the Fertilization Process	139:163	Glycoproteins Presenting Galactose and N-Acetylgalactosamine in Human Seminal Plasma as Potential Players Involved in Immune Modulation in the Fertilization Process.
34298952	9	14	theme	Gal/GalNAc	1790:1799	arg1	lectins					1810:1816	endogenous Gal/GalNAc specific lectins	1779:1816	endogenous Gal/GalNAc specific lectins with a possible role in modulation of maternal immune response during fertilization	1779:1900	Overall, our research revealed potential seminal plasma ligands for endogenous Gal/GalNAc specific lectins with a possible role in modulation of maternal immune response during fertilization.
34298952	4	15	theme	immunomodulatory	736:751	arg1	glycans					753:759	immunomodulatory glycans	736:759	immunomodulatory glycans	736:759	Among immunomodulatory glycans, Lewis type antigens have been of interest for at least two decades, while the importance of T/Tn antigens and related structures is still far from understanding.
34298952	2	16	theme	endogenous	588:597	arg1	lectins					599:605	endogenous lectins	588:605	endogenous lectins that decorate the surface of immune cells	588:647	Seminal plasma glycoproteins are rich in the unique immunomodulatory glycoepitopes that may serve as ligands for endogenous lectins that decorate the surface of immune cells.
34298952	3	17	theme	Such	650:653	arg1	interaction					655:665	Such interaction	650:665	Such interaction	650:665	Such interaction may be involved in modulation of the maternal immune response.
34298952	2	18	from	glycoepitopes	544:556	arg1	rich					508:511	rich	508:511	rich	508:511	Seminal plasma glycoproteins are rich in the unique immunomodulatory glycoepitopes that may serve as ligands for endogenous lectins that decorate the surface of immune cells.
34298952	6	19	theme	prolactin	1218:1226	arg1	protein					1238:1244	prolactin inducible protein	1218:1244	prolactin inducible protein	1218:1244	By means of lectin blotting and lectin affinity chromatography followed by LC-MS, we identified lactotransferrin, prolactin inducible protein as well as fibronectin and semenogelins 1 and 2 as lectin-reactive.
34298952	6	19	theme	prolactin	1218:1226	arg1	lactotransferrin					1200:1215	lactotransferrin	1200:1215	lactotransferrin	1200:1215	By means of lectin blotting and lectin affinity chromatography followed by LC-MS, we identified lactotransferrin, prolactin inducible protein as well as fibronectin and semenogelins 1 and 2 as lectin-reactive.
34298952	1	20	contain	have	268:271	arg2	impact					287:292	a significant impact	273:292	a significant impact	273:292	In light of recent research, there is increasing evidence showing that extracellular semen components have a significant impact on the immune reaction of the female partner, leading to the tolerogenic response enabling the embryo development and implantation as well as further progress of healthy pregnancy.
34298952	1	20	contain	have	268:271	arg1	components					257:266	extracellular semen components	237:266	extracellular semen components	237:266	In light of recent research, there is increasing evidence showing that extracellular semen components have a significant impact on the immune reaction of the female partner, leading to the tolerogenic response enabling the embryo development and implantation as well as further progress of healthy pregnancy.
34298952	0	21	from	Galactose	25:33	arg1	Plasma					78:83	Human Seminal Plasma	64:83	Human Seminal Plasma	64:83	Glycoproteins Presenting Galactose and N-Acetylgalactosamine in Human Seminal Plasma as Potential Players Involved in Immune Modulation in the Fertilization Process.
34298952	0	22	theme	Potential	88:96	arg1	Galactose					25:33	Galactose	25:33	Galactose	25:33	Glycoproteins Presenting Galactose and N-Acetylgalactosamine in Human Seminal Plasma as Potential Players Involved in Immune Modulation in the Fertilization Process.
34298952	0	22	theme	Potential	88:96	arg1	Players					98:104	Potential Players	88:104	Potential Players Involved in Immune Modulation in the Fertilization Process	88:163	Glycoproteins Presenting Galactose and N-Acetylgalactosamine in Human Seminal Plasma as Potential Players Involved in Immune Modulation in the Fertilization Process.
34298952	0	22	theme	Potential	88:96	arg1	N-Acetylgalactosamine					39:59	N-Acetylgalactosamine	39:59	N-Acetylgalactosamine	39:59	Glycoproteins Presenting Galactose and N-Acetylgalactosamine in Human Seminal Plasma as Potential Players Involved in Immune Modulation in the Fertilization Process.
34298952	8	23	theme	STRING	1553:1558	arg1	analysis					1575:1582	STRING bioinformatics analysis	1553:1582	STRING bioinformatics analysis	1553:1582	STRING bioinformatics analysis linked the identified glycoproteins in the close network, indicating their involvement in immune (partially innate) processes.
34298952	1	24	theme	tolerogenic	355:365	arg1	response					367:374	the tolerogenic response	351:374	the tolerogenic response enabling the embryo development and implantation as well as further progress of healthy pregnancy	351:472	In light of recent research, there is increasing evidence showing that extracellular semen components have a significant impact on the immune reaction of the female partner, leading to the tolerogenic response enabling the embryo development and implantation as well as further progress of healthy pregnancy.
34298952	6	25	theme	inducible	1228:1236	arg1	protein					1238:1244	prolactin inducible protein	1218:1244	prolactin inducible protein	1218:1244	By means of lectin blotting and lectin affinity chromatography followed by LC-MS, we identified lactotransferrin, prolactin inducible protein as well as fibronectin and semenogelins 1 and 2 as lectin-reactive.
34298952	6	25	theme	inducible	1228:1236	arg1	lactotransferrin					1200:1215	lactotransferrin	1200:1215	lactotransferrin	1200:1215	By means of lectin blotting and lectin affinity chromatography followed by LC-MS, we identified lactotransferrin, prolactin inducible protein as well as fibronectin and semenogelins 1 and 2 as lectin-reactive.
34298952	2	26	theme	immunomodulatory	527:542	arg1	ligands					576:582	ligands	576:582	ligands for endogenous lectins that decorate the surface of immune cells	576:647	Seminal plasma glycoproteins are rich in the unique immunomodulatory glycoepitopes that may serve as ligands for endogenous lectins that decorate the surface of immune cells.
34298952	2	26	theme	immunomodulatory	527:542	arg1	glycoepitopes					544:556	the unique immunomodulatory glycoepitopes	516:556	the unique immunomodulatory glycoepitopes that may serve as ligands for endogenous lectins that decorate the surface of immune cells	516:647	Seminal plasma glycoproteins are rich in the unique immunomodulatory glycoepitopes that may serve as ligands for endogenous lectins that decorate the surface of immune cells.
34298952	9	27	with	lectins	1810:1816	arg1	role					1834:1837	a possible role	1823:1837	a possible role in modulation of maternal immune response during fertilization	1823:1900	Overall, our research revealed potential seminal plasma ligands for endogenous Gal/GalNAc specific lectins with a possible role in modulation of maternal immune response during fertilization.
34298952	0	28	theme	Immune	118:123	arg1	Modulation					125:134	Immune Modulation	118:134	Immune Modulation in the Fertilization Process	118:163	Glycoproteins Presenting Galactose and N-Acetylgalactosamine in Human Seminal Plasma as Potential Players Involved in Immune Modulation in the Fertilization Process.
34298952	9	29	theme	response	1872:1879	arg1	modulation					1842:1851	modulation	1842:1851	modulation of maternal immune response	1842:1879	Overall, our research revealed potential seminal plasma ligands for endogenous Gal/GalNAc specific lectins with a possible role in modulation of maternal immune response during fertilization.
34298952	0	30	from	N-Acetylgalactosamine	39:59	arg1	Plasma					78:83	Human Seminal Plasma	64:83	Human Seminal Plasma	64:83	Glycoproteins Presenting Galactose and N-Acetylgalactosamine in Human Seminal Plasma as Potential Players Involved in Immune Modulation in the Fertilization Process.
34298952	4	31	theme	type	768:771	arg1	antigens					773:780	Lewis type antigens	762:780	Lewis type antigens	762:780	Among immunomodulatory glycans, Lewis type antigens have been of interest for at least two decades, while the importance of T/Tn antigens and related structures is still far from understanding.
34298952	9	32	theme	potential	1742:1750	arg1	ligands					1767:1773	potential seminal plasma ligands	1742:1773	potential seminal plasma ligands for endogenous Gal/GalNAc specific lectins with a possible role in modulation of maternal immune response during fertilization	1742:1900	Overall, our research revealed potential seminal plasma ligands for endogenous Gal/GalNAc specific lectins with a possible role in modulation of maternal immune response during fertilization.
34298952	1	33	theme	immune	301:306	arg1	reaction					308:315	the immune reaction	297:315	the immune reaction of the female partner	297:337	In light of recent research, there is increasing evidence showing that extracellular semen components have a significant impact on the immune reaction of the female partner, leading to the tolerogenic response enabling the embryo development and implantation as well as further progress of healthy pregnancy.
34298952	2	34	gly	glycoproteins	490:502	arg1	glycoproteins					490:502	Seminal plasma glycoproteins	475:502	Seminal plasma glycoproteins	475:502	Seminal plasma glycoproteins are rich in the unique immunomodulatory glycoepitopes that may serve as ligands for endogenous lectins that decorate the surface of immune cells.
34298952	7	35	theme	analysis	1334:1341	arg1	results					1343:1349	Net-O-glycosylation analysis results	1314:1349	Net-O-glycosylation analysis results	1314:1349	Net-O-glycosylation analysis results indicated that the latter three may actually carry T and/or Tn antigens, while in the case of prolactin inducible protein and lactotransferrin LacdiNAc and lactosamine glycoepitopes were more probable.
34298952	2	36	theme	Seminal	475:481	arg1	glycoproteins					490:502	Seminal plasma glycoproteins	475:502	Seminal plasma glycoproteins	475:502	Seminal plasma glycoproteins are rich in the unique immunomodulatory glycoepitopes that may serve as ligands for endogenous lectins that decorate the surface of immune cells.
34298952	0	37	theme	Human	64:68	arg1	Plasma					78:83	Human Seminal Plasma	64:83	Human Seminal Plasma	64:83	Glycoproteins Presenting Galactose and N-Acetylgalactosamine in Human Seminal Plasma as Potential Players Involved in Immune Modulation in the Fertilization Process.
34298952	1	38	theme	female	324:329	arg1	partner					331:337	the female partner	320:337	the female partner	320:337	In light of recent research, there is increasing evidence showing that extracellular semen components have a significant impact on the immune reaction of the female partner, leading to the tolerogenic response enabling the embryo development and implantation as well as further progress of healthy pregnancy.
34298952	9	39	from	role	1834:1837	arg1	modulation					1842:1851	modulation	1842:1851	modulation of maternal immune response	1842:1879	Overall, our research revealed potential seminal plasma ligands for endogenous Gal/GalNAc specific lectins with a possible role in modulation of maternal immune response during fertilization.
34298952	6	40	theme	lectin	1136:1141	arg1	chromatography					1152:1165	lectin affinity chromatography	1136:1165	lectin affinity chromatography	1136:1165	By means of lectin blotting and lectin affinity chromatography followed by LC-MS, we identified lactotransferrin, prolactin inducible protein as well as fibronectin and semenogelins 1 and 2 as lectin-reactive.
34298952	5	41	theme	glycoepitopes	1000:1012	arg1	capable					974:980	capable	974:980	capable	974:980	In the current work, we applied two plant lectins capable of distinguishing glycoepitopes with terminal GalNAc and Gal to identify glycoproteins that are their efficient carriers.
34298952	1	42	theme	semen	251:255	arg1	components					257:266	extracellular semen components	237:266	extracellular semen components	237:266	In light of recent research, there is increasing evidence showing that extracellular semen components have a significant impact on the immune reaction of the female partner, leading to the tolerogenic response enabling the embryo development and implantation as well as further progress of healthy pregnancy.
34298952	5	43	theme	terminal	1019:1026	arg1	GalNAc					1028:1033	terminal GalNAc	1019:1033	terminal GalNAc	1019:1033	In the current work, we applied two plant lectins capable of distinguishing glycoepitopes with terminal GalNAc and Gal to identify glycoproteins that are their efficient carriers.
34298952	5	44	gly	glycoproteins	1055:1067	arg1	carriers					1094:1101	their efficient carriers	1078:1101	their efficient carriers	1078:1101	In the current work, we applied two plant lectins capable of distinguishing glycoepitopes with terminal GalNAc and Gal to identify glycoproteins that are their efficient carriers.
34298952	5	44	gly	glycoproteins	1055:1067	arg1	glycoproteins					1055:1067	glycoproteins	1055:1067	glycoproteins that are their efficient carriers	1055:1101	In the current work, we applied two plant lectins capable of distinguishing glycoepitopes with terminal GalNAc and Gal to identify glycoproteins that are their efficient carriers.
34298952	8	45	theme	immune	1674:1679	arg1	processes					1700:1708	immune (partially innate) processes	1674:1708	immune (partially innate) processes	1674:1708	STRING bioinformatics analysis linked the identified glycoproteins in the close network, indicating their involvement in immune (partially innate) processes.
34298952	7	46	theme	lactosamine	1507:1517	arg1	glycoepitopes					1519:1531	lactosamine glycoepitopes	1507:1531	lactosamine glycoepitopes	1507:1531	Net-O-glycosylation analysis results indicated that the latter three may actually carry T and/or Tn antigens, while in the case of prolactin inducible protein and lactotransferrin LacdiNAc and lactosamine glycoepitopes were more probable.
34298952	4	47	theme	related	872:878	arg1	structures					880:889	related structures	872:889	related structures	872:889	Among immunomodulatory glycans, Lewis type antigens have been of interest for at least two decades, while the importance of T/Tn antigens and related structures is still far from understanding.
34298952	3	48	theme	immune	713:718	arg1	response					720:727	the maternal immune response	700:727	the maternal immune response	700:727	Such interaction may be involved in modulation of the maternal immune response.
34298952	1	49	theme	significant	275:285	arg1	impact					287:292	a significant impact	273:292	a significant impact	273:292	In light of recent research, there is increasing evidence showing that extracellular semen components have a significant impact on the immune reaction of the female partner, leading to the tolerogenic response enabling the embryo development and implantation as well as further progress of healthy pregnancy.
34298952	7	50	theme	LacdiNAc	1494:1501	arg1	glycoepitopes					1519:1531	lactosamine glycoepitopes	1507:1531	lactosamine glycoepitopes	1507:1531	Net-O-glycosylation analysis results indicated that the latter three may actually carry T and/or Tn antigens, while in the case of prolactin inducible protein and lactotransferrin LacdiNAc and lactosamine glycoepitopes were more probable.
34298952	7	50	theme	LacdiNAc	1494:1501	arg1	case					1437:1440	the case	1433:1440	the case of prolactin inducible protein and lactotransferrin LacdiNAc	1433:1501	Net-O-glycosylation analysis results indicated that the latter three may actually carry T and/or Tn antigens, while in the case of prolactin inducible protein and lactotransferrin LacdiNAc and lactosamine glycoepitopes were more probable.
34298952	7	51	theme	protein	1465:1471	arg1	glycoepitopes					1519:1531	lactosamine glycoepitopes	1507:1531	lactosamine glycoepitopes	1507:1531	Net-O-glycosylation analysis results indicated that the latter three may actually carry T and/or Tn antigens, while in the case of prolactin inducible protein and lactotransferrin LacdiNAc and lactosamine glycoepitopes were more probable.
34298952	7	51	theme	protein	1465:1471	arg1	case					1437:1440	the case	1433:1440	the case of prolactin inducible protein and lactotransferrin LacdiNAc	1433:1501	Net-O-glycosylation analysis results indicated that the latter three may actually carry T and/or Tn antigens, while in the case of prolactin inducible protein and lactotransferrin LacdiNAc and lactosamine glycoepitopes were more probable.
34298952	1	52	theme	pregnancy	464:472	arg1	progress					444:451	further progress	436:451	further progress of healthy pregnancy	436:472	In light of recent research, there is increasing evidence showing that extracellular semen components have a significant impact on the immune reaction of the female partner, leading to the tolerogenic response enabling the embryo development and implantation as well as further progress of healthy pregnancy.
34298952	1	52	theme	pregnancy	464:472	arg1	implantation					412:423	implantation	412:423	implantation	412:423	In light of recent research, there is increasing evidence showing that extracellular semen components have a significant impact on the immune reaction of the female partner, leading to the tolerogenic response enabling the embryo development and implantation as well as further progress of healthy pregnancy.
34298952	1	52	theme	pregnancy	464:472	arg1	development					396:406	the embryo development	385:406	the embryo development	385:406	In light of recent research, there is increasing evidence showing that extracellular semen components have a significant impact on the immune reaction of the female partner, leading to the tolerogenic response enabling the embryo development and implantation as well as further progress of healthy pregnancy.
34298952	1	53	theme	recent	178:183	arg1	research					185:192	recent research	178:192	recent research	178:192	In light of recent research, there is increasing evidence showing that extracellular semen components have a significant impact on the immune reaction of the female partner, leading to the tolerogenic response enabling the embryo development and implantation as well as further progress of healthy pregnancy.
34298952	7	54	theme	prolactin	1445:1453	arg1	protein					1465:1471	prolactin inducible protein	1445:1471	prolactin inducible protein	1445:1471	Net-O-glycosylation analysis results indicated that the latter three may actually carry T and/or Tn antigens, while in the case of prolactin inducible protein and lactotransferrin LacdiNAc and lactosamine glycoepitopes were more probable.
34298952	2	55	theme	immune	636:641	arg1	cells					643:647	immune cells	636:647	immune cells	636:647	Seminal plasma glycoproteins are rich in the unique immunomodulatory glycoepitopes that may serve as ligands for endogenous lectins that decorate the surface of immune cells.
34298952	2	56	from	rich	508:511	arg1	ligands					576:582	ligands	576:582	ligands for endogenous lectins that decorate the surface of immune cells	576:647	Seminal plasma glycoproteins are rich in the unique immunomodulatory glycoepitopes that may serve as ligands for endogenous lectins that decorate the surface of immune cells.
34298952	2	56	from	rich	508:511	arg1	glycoepitopes					544:556	the unique immunomodulatory glycoepitopes	516:556	the unique immunomodulatory glycoepitopes that may serve as ligands for endogenous lectins that decorate the surface of immune cells	516:647	Seminal plasma glycoproteins are rich in the unique immunomodulatory glycoepitopes that may serve as ligands for endogenous lectins that decorate the surface of immune cells.
34298952	9	57	theme	seminal	1752:1758	arg1	ligands					1767:1773	potential seminal plasma ligands	1742:1773	potential seminal plasma ligands for endogenous Gal/GalNAc specific lectins with a possible role in modulation of maternal immune response during fertilization	1742:1900	Overall, our research revealed potential seminal plasma ligands for endogenous Gal/GalNAc specific lectins with a possible role in modulation of maternal immune response during fertilization.
34298952	7	58	from	glycoepitopes	1519:1531	arg1	probable					1543:1550	probable	1543:1550	probable	1543:1550	Net-O-glycosylation analysis results indicated that the latter three may actually carry T and/or Tn antigens, while in the case of prolactin inducible protein and lactotransferrin LacdiNAc and lactosamine glycoepitopes were more probable.
34298952	9	59	theme	endogenous	1779:1788	arg1	lectins					1810:1816	endogenous Gal/GalNAc specific lectins	1779:1816	endogenous Gal/GalNAc specific lectins with a possible role in modulation of maternal immune response during fertilization	1779:1900	Overall, our research revealed potential seminal plasma ligands for endogenous Gal/GalNAc specific lectins with a possible role in modulation of maternal immune response during fertilization.
34298952	9	60	theme	specific	1801:1808	arg1	lectins					1810:1816	endogenous Gal/GalNAc specific lectins	1779:1816	endogenous Gal/GalNAc specific lectins with a possible role in modulation of maternal immune response during fertilization	1779:1900	Overall, our research revealed potential seminal plasma ligands for endogenous Gal/GalNAc specific lectins with a possible role in modulation of maternal immune response during fertilization.
34298952	0	61	from	Modulation	125:134	arg1	Process					157:163	the Fertilization Process	139:163	the Fertilization Process	139:163	Glycoproteins Presenting Galactose and N-Acetylgalactosamine in Human Seminal Plasma as Potential Players Involved in Immune Modulation in the Fertilization Process.
34298952	7	62	theme	Tn	1411:1412	arg1	antigens					1414:1421	Tn antigens	1411:1421	Tn antigens	1411:1421	Net-O-glycosylation analysis results indicated that the latter three may actually carry T and/or Tn antigens, while in the case of prolactin inducible protein and lactotransferrin LacdiNAc and lactosamine glycoepitopes were more probable.
34298952	5	63	theme	current	931:937	arg1	work					939:942	the current work	927:942	the current work	927:942	In the current work, we applied two plant lectins capable of distinguishing glycoepitopes with terminal GalNAc and Gal to identify glycoproteins that are their efficient carriers.
34298952	5	64	theme	plant	960:964	arg1	lectins					966:972	two plant lectins	956:972	two plant lectins capable of distinguishing glycoepitopes with terminal GalNAc and Gal	956:1041	In the current work, we applied two plant lectins capable of distinguishing glycoepitopes with terminal GalNAc and Gal to identify glycoproteins that are their efficient carriers.
34298952	1	65	theme	further	436:442	arg1	progress					444:451	further progress	436:451	further progress of healthy pregnancy	436:472	In light of recent research, there is increasing evidence showing that extracellular semen components have a significant impact on the immune reaction of the female partner, leading to the tolerogenic response enabling the embryo development and implantation as well as further progress of healthy pregnancy.
34298952	7	66	from	case	1437:1440	arg1	probable					1543:1550	probable	1543:1550	probable	1543:1550	Net-O-glycosylation analysis results indicated that the latter three may actually carry T and/or Tn antigens, while in the case of prolactin inducible protein and lactotransferrin LacdiNAc and lactosamine glycoepitopes were more probable.
34298952	9	67	theme	possible	1825:1832	arg1	role					1834:1837	a possible role	1823:1837	a possible role in modulation of maternal immune response during fertilization	1823:1900	Overall, our research revealed potential seminal plasma ligands for endogenous Gal/GalNAc specific lectins with a possible role in modulation of maternal immune response during fertilization.
34298952	8	68	theme	bioinformatics	1560:1573	arg1	analysis					1575:1582	STRING bioinformatics analysis	1553:1582	STRING bioinformatics analysis	1553:1582	STRING bioinformatics analysis linked the identified glycoproteins in the close network, indicating their involvement in immune (partially innate) processes.
34298952	8	69	gly	glycoproteins	1606:1618	arg1	glycoproteins					1606:1618	the identified glycoproteins	1591:1618	the identified glycoproteins in the close network	1591:1639	STRING bioinformatics analysis linked the identified glycoproteins in the close network, indicating their involvement in immune (partially innate) processes.
34298952	8	70	dep	immune	1674:1679	arg1	innate					1692:1697	innate	1692:1697	innate	1692:1697	STRING bioinformatics analysis linked the identified glycoproteins in the close network, indicating their involvement in immune (partially innate) processes.
34298952	5	71	theme	capable	974:980	arg1	lectins					966:972	two plant lectins	956:972	two plant lectins capable of distinguishing glycoepitopes with terminal GalNAc and Gal	956:1041	In the current work, we applied two plant lectins capable of distinguishing glycoepitopes with terminal GalNAc and Gal to identify glycoproteins that are their efficient carriers.
34298952	2	72	theme	unique	520:525	arg1	ligands					576:582	ligands	576:582	ligands for endogenous lectins that decorate the surface of immune cells	576:647	Seminal plasma glycoproteins are rich in the unique immunomodulatory glycoepitopes that may serve as ligands for endogenous lectins that decorate the surface of immune cells.
34298952	2	72	theme	unique	520:525	arg1	glycoepitopes					544:556	the unique immunomodulatory glycoepitopes	516:556	the unique immunomodulatory glycoepitopes that may serve as ligands for endogenous lectins that decorate the surface of immune cells	516:647	Seminal plasma glycoproteins are rich in the unique immunomodulatory glycoepitopes that may serve as ligands for endogenous lectins that decorate the surface of immune cells.
34298952	8	73	theme	identified	1595:1604	arg1	glycoproteins					1606:1618	the identified glycoproteins	1591:1618	the identified glycoproteins in the close network	1591:1639	STRING bioinformatics analysis linked the identified glycoproteins in the close network, indicating their involvement in immune (partially innate) processes.
34298952	9	74	theme	maternal	1856:1863	arg1	response					1872:1879	maternal immune response	1856:1879	maternal immune response	1856:1879	Overall, our research revealed potential seminal plasma ligands for endogenous Gal/GalNAc specific lectins with a possible role in modulation of maternal immune response during fertilization.
34298952	4	75	theme	Lewis	762:766	arg1	antigens					773:780	Lewis type antigens	762:780	Lewis type antigens	762:780	Among immunomodulatory glycans, Lewis type antigens have been of interest for at least two decades, while the importance of T/Tn antigens and related structures is still far from understanding.
34298952	5	76	theme	efficient	1084:1092	arg1	carriers					1094:1101	their efficient carriers	1078:1101	their efficient carriers	1078:1101	In the current work, we applied two plant lectins capable of distinguishing glycoepitopes with terminal GalNAc and Gal to identify glycoproteins that are their efficient carriers.
34298952	5	76	theme	efficient	1084:1092	arg1	glycoproteins					1055:1067	glycoproteins	1055:1067	glycoproteins that are their efficient carriers	1055:1101	In the current work, we applied two plant lectins capable of distinguishing glycoepitopes with terminal GalNAc and Gal to identify glycoproteins that are their efficient carriers.
34298952	8	77	theme	close	1627:1631	arg1	network					1633:1639	the close network	1623:1639	the close network	1623:1639	STRING bioinformatics analysis linked the identified glycoproteins in the close network, indicating their involvement in immune (partially innate) processes.
34298952	7	78	theme	Net-O-glycosylation	1314:1332	arg1	results					1343:1349	Net-O-glycosylation analysis results	1314:1349	Net-O-glycosylation analysis results	1314:1349	Net-O-glycosylation analysis results indicated that the latter three may actually carry T and/or Tn antigens, while in the case of prolactin inducible protein and lactotransferrin LacdiNAc and lactosamine glycoepitopes were more probable.
34298952	2	79	theme	plasma	483:488	arg1	glycoproteins					490:502	Seminal plasma glycoproteins	475:502	Seminal plasma glycoproteins	475:502	Seminal plasma glycoproteins are rich in the unique immunomodulatory glycoepitopes that may serve as ligands for endogenous lectins that decorate the surface of immune cells.
34298952	9	80	theme	immune	1865:1870	arg1	response					1872:1879	maternal immune response	1856:1879	maternal immune response	1856:1879	Overall, our research revealed potential seminal plasma ligands for endogenous Gal/GalNAc specific lectins with a possible role in modulation of maternal immune response during fertilization.
34298952	0	81	theme	Seminal	70:76	arg1	Plasma					78:83	Human Seminal Plasma	64:83	Human Seminal Plasma	64:83	Glycoproteins Presenting Galactose and N-Acetylgalactosamine in Human Seminal Plasma as Potential Players Involved in Immune Modulation in the Fertilization Process.
34298952	4	82	theme	antigens	859:866	arg1	understanding					909:921	understanding	909:921	understanding	909:921	Among immunomodulatory glycans, Lewis type antigens have been of interest for at least two decades, while the importance of T/Tn antigens and related structures is still far from understanding.
34298952	4	82	theme	antigens	859:866	arg1	importance					840:849	the importance	836:849	the importance of T/Tn antigens and related structures	836:889	Among immunomodulatory glycans, Lewis type antigens have been of interest for at least two decades, while the importance of T/Tn antigens and related structures is still far from understanding.
34298952	6	83	theme	affinity	1143:1150	arg1	chromatography					1152:1165	lectin affinity chromatography	1136:1165	lectin affinity chromatography	1136:1165	By means of lectin blotting and lectin affinity chromatography followed by LC-MS, we identified lactotransferrin, prolactin inducible protein as well as fibronectin and semenogelins 1 and 2 as lectin-reactive.
34298952	8	84	from	involvement	1659:1669	arg1	processes					1700:1708	immune (partially innate) processes	1674:1708	immune (partially innate) processes	1674:1708	STRING bioinformatics analysis linked the identified glycoproteins in the close network, indicating their involvement in immune (partially innate) processes.
34298952	1	85	theme	partner	331:337	arg1	reaction					308:315	the immune reaction	297:315	the immune reaction of the female partner	297:337	In light of recent research, there is increasing evidence showing that extracellular semen components have a significant impact on the immune reaction of the female partner, leading to the tolerogenic response enabling the embryo development and implantation as well as further progress of healthy pregnancy.
34298952	5	86	theme	distinguishing	985:998	arg1	glycoepitopes					1000:1012	distinguishing glycoepitopes	985:1012	distinguishing glycoepitopes with terminal GalNAc and Gal	985:1041	In the current work, we applied two plant lectins capable of distinguishing glycoepitopes with terminal GalNAc and Gal to identify glycoproteins that are their efficient carriers.
34298952	6	87	dep	blotting	1123:1130	arg1	means					1107:1111	means	1107:1111	means	1107:1111	By means of lectin blotting and lectin affinity chromatography followed by LC-MS, we identified lactotransferrin, prolactin inducible protein as well as fibronectin and semenogelins 1 and 2 as lectin-reactive.
34298952	1	88	theme	extracellular	237:249	arg1	components					257:266	extracellular semen components	237:266	extracellular semen components	237:266	In light of recent research, there is increasing evidence showing that extracellular semen components have a significant impact on the immune reaction of the female partner, leading to the tolerogenic response enabling the embryo development and implantation as well as further progress of healthy pregnancy.
34298952	7	89	from	probable	1543:1550	arg1	glycoepitopes					1519:1531	lactosamine glycoepitopes	1507:1531	lactosamine glycoepitopes	1507:1531	Net-O-glycosylation analysis results indicated that the latter three may actually carry T and/or Tn antigens, while in the case of prolactin inducible protein and lactotransferrin LacdiNAc and lactosamine glycoepitopes were more probable.
34298952	7	89	from	probable	1543:1550	arg1	case					1437:1440	the case	1433:1440	the case of prolactin inducible protein and lactotransferrin LacdiNAc	1433:1501	Net-O-glycosylation analysis results indicated that the latter three may actually carry T and/or Tn antigens, while in the case of prolactin inducible protein and lactotransferrin LacdiNAc and lactosamine glycoepitopes were more probable.
34298952	6	90	theme	lectin	1116:1121	arg1	blotting					1123:1130	lectin blotting	1116:1130	lectin blotting	1116:1130	By means of lectin blotting and lectin affinity chromatography followed by LC-MS, we identified lactotransferrin, prolactin inducible protein as well as fibronectin and semenogelins 1 and 2 as lectin-reactive.
34298952	3	91	theme	response	720:727	arg1	modulation					686:695	modulation	686:695	modulation of the maternal immune response	686:727	Such interaction may be involved in modulation of the maternal immune response.
34298952	7	92	contain	carry	1396:1400	arg2	antigens					1414:1421	Tn antigens	1411:1421	Tn antigens	1411:1421	Net-O-glycosylation analysis results indicated that the latter three may actually carry T and/or Tn antigens, while in the case of prolactin inducible protein and lactotransferrin LacdiNAc and lactosamine glycoepitopes were more probable.
34298952	7	92	contain	carry	1396:1400	arg2	T					1402:1402	T	1402:1402	T	1402:1402	Net-O-glycosylation analysis results indicated that the latter three may actually carry T and/or Tn antigens, while in the case of prolactin inducible protein and lactotransferrin LacdiNAc and lactosamine glycoepitopes were more probable.
34298952	7	92	contain	carry	1396:1400	arg1	three					1377:1381	three	1377:1381	three	1377:1381	Net-O-glycosylation analysis results indicated that the latter three may actually carry T and/or Tn antigens, while in the case of prolactin inducible protein and lactotransferrin LacdiNAc and lactosamine glycoepitopes were more probable.
32522869	5	0	theme	SELP/CNF	879:886	arg1	actuators					888:896	the SELP/CNF actuators	875:896	the SELP/CNF actuators	875:896	The reversible deformation performance of the SELP/CNF actuators was quantified, and complex spatial transformations of multilayer actuators were demonstrated, including a biomimetic flower design with selective petal movements.
32522869	4	1	theme	three-dimensional	789:805	arg1	shapes					825:830	three-dimensional (3D) origami-like shapes	789:830	three-dimensional (3D) origami-like shapes	789:830	Programmed site-selective actuation can be predicted and folded into three-dimensional (3D) origami-like shapes.
32522869	5	2	theme	multilayer	953:962	arg1	actuators					964:972	multilayer actuators	953:972	multilayer actuators	953:972	The reversible deformation performance of the SELP/CNF actuators was quantified, and complex spatial transformations of multilayer actuators were demonstrated, including a biomimetic flower design with selective petal movements.
32522869	5	3	theme	actuators	888:896	arg1	performance					860:870	The reversible deformation performance	833:870	The reversible deformation performance of the SELP/CNF actuators	833:896	The reversible deformation performance of the SELP/CNF actuators was quantified, and complex spatial transformations of multilayer actuators were demonstrated, including a biomimetic flower design with selective petal movements.
32522869	3	4	theme	wood-derived	595:606	arg1	CNFs					630:633	CNFs	630:633	CNFs	630:633	Herein, we report a bionic actuator system consisting of stimuli-responsive genetically engineered silk-elastin-like protein (SELP) hydrogels and wood-derived cellulose nanofibers (CNFs), which respond to temperature and ionic strength underwater by ecofriendly methods.
32522869	3	4	theme	wood-derived	595:606	arg1	nanofibers					618:627	wood-derived cellulose nanofibers	595:627	wood-derived cellulose nanofibers (CNFs)	595:634	Herein, we report a bionic actuator system consisting of stimuli-responsive genetically engineered silk-elastin-like protein (SELP) hydrogels and wood-derived cellulose nanofibers (CNFs), which respond to temperature and ionic strength underwater by ecofriendly methods.
32522869	6	5	dep	in	1214:1215	arg1	vivo					1217:1220	vivo	1217:1220	vivo	1217:1220	Such actuators consisting entirely of biocompatible and biodegradable materials will offer an option toward constructing stimuli-responsive systems for in vivo biomedicine soft robotics and bionic research.
32522869	6	6	dep	biomedicine	1222:1232	arg1	robotics					1239:1246	soft robotics	1234:1246	soft robotics	1234:1246	Such actuators consisting entirely of biocompatible and biodegradable materials will offer an option toward constructing stimuli-responsive systems for in vivo biomedicine soft robotics and bionic research.
32522869	5	7	theme	reversible	837:846	arg1	performance					860:870	The reversible deformation performance	833:870	The reversible deformation performance of the SELP/CNF actuators	833:896	The reversible deformation performance of the SELP/CNF actuators was quantified, and complex spatial transformations of multilayer actuators were demonstrated, including a biomimetic flower design with selective petal movements.
32522869	5	8	with	design	1023:1028	arg1	movements					1051:1059	selective petal movements	1035:1059	selective petal movements	1035:1059	The reversible deformation performance of the SELP/CNF actuators was quantified, and complex spatial transformations of multilayer actuators were demonstrated, including a biomimetic flower design with selective petal movements.
32522869	5	9	theme	deformation	848:858	arg1	performance					860:870	The reversible deformation performance	833:870	The reversible deformation performance of the SELP/CNF actuators	833:896	The reversible deformation performance of the SELP/CNF actuators was quantified, and complex spatial transformations of multilayer actuators were demonstrated, including a biomimetic flower design with selective petal movements.
32522869	5	10	theme	petal	1045:1049	arg1	movements					1051:1059	selective petal movements	1035:1059	selective petal movements	1035:1059	The reversible deformation performance of the SELP/CNF actuators was quantified, and complex spatial transformations of multilayer actuators were demonstrated, including a biomimetic flower design with selective petal movements.
32522869	5	11	theme	actuators	964:972	arg1	transformations					934:948	complex spatial transformations	918:948	complex spatial transformations of multilayer actuators	918:972	The reversible deformation performance of the SELP/CNF actuators was quantified, and complex spatial transformations of multilayer actuators were demonstrated, including a biomimetic flower design with selective petal movements.
32522869	4	12	theme	Programmed	720:729	arg1	actuation					746:754	Programmed site-selective actuation	720:754	Programmed site-selective actuation	720:754	Programmed site-selective actuation can be predicted and folded into three-dimensional (3D) origami-like shapes.
32522869	1	13	theme	Bioinspired	95:105	arg1	actuators					107:115	Bioinspired actuators	95:115	Bioinspired actuators with stimuli-responsive and deformable properties	95:165	Bioinspired actuators with stimuli-responsive and deformable properties are being pursued in fields such as artificial tissues, medical devices and diagnostics, and intelligent biosensors.
32522869	1	14	theme	artificial	203:212	arg1	tissues					214:220	artificial tissues	203:220	artificial tissues	203:220	Bioinspired actuators with stimuli-responsive and deformable properties are being pursued in fields such as artificial tissues, medical devices and diagnostics, and intelligent biosensors.
32522869	5	15	theme	selective	1035:1043	arg1	movements					1051:1059	selective petal movements	1035:1059	selective petal movements	1035:1059	The reversible deformation performance of the SELP/CNF actuators was quantified, and complex spatial transformations of multilayer actuators were demonstrated, including a biomimetic flower design with selective petal movements.
32522869	3	16	theme	bionic	469:474	arg1	system					485:490	a bionic actuator system	467:490	a bionic actuator system consisting of stimuli-responsive genetically engineered silk-elastin-like protein (SELP) hydrogels and wood-derived cellulose nanofibers (CNFs), which respond to temperature and ionic strength underwater by ecofriendly methods	467:717	Herein, we report a bionic actuator system consisting of stimuli-responsive genetically engineered silk-elastin-like protein (SELP) hydrogels and wood-derived cellulose nanofibers (CNFs), which respond to temperature and ionic strength underwater by ecofriendly methods.
32522869	2	17	theme	actuator	316:323	arg1	systems					325:331	actuator systems	316:331	actuator systems	316:331	These applications require that actuator systems have biocompatibility, controlled deformability, biodegradability, mechanical durability, and stable reversibility.
32522869	3	18	theme	stimuli-responsive	506:523	arg1	hydrogels					581:589	stimuli-responsive genetically engineered silk-elastin-like protein (SELP) hydrogels	506:589	stimuli-responsive genetically engineered silk-elastin-like protein (SELP) hydrogels	506:589	Herein, we report a bionic actuator system consisting of stimuli-responsive genetically engineered silk-elastin-like protein (SELP) hydrogels and wood-derived cellulose nanofibers (CNFs), which respond to temperature and ionic strength underwater by ecofriendly methods.
32522869	0	19	theme	composite	19:27	arg1	biopolymer					29:38	Stimuli-responsive composite biopolymer	0:38	Stimuli-responsive composite biopolymer	0:38	Stimuli-responsive composite biopolymer actuators with selective spatial deformation behavior.
32522869	6	20	theme	bionic	1252:1257	arg1	research					1259:1266	bionic research	1252:1266	bionic research	1252:1266	Such actuators consisting entirely of biocompatible and biodegradable materials will offer an option toward constructing stimuli-responsive systems for in vivo biomedicine soft robotics and bionic research.
32522869	0	21	theme	Stimuli-responsive	0:17	arg1	biopolymer					29:38	Stimuli-responsive composite biopolymer	0:38	Stimuli-responsive composite biopolymer	0:38	Stimuli-responsive composite biopolymer actuators with selective spatial deformation behavior.
32522869	4	22	dep	three-dimensional	789:805	arg1	3D					808:809	3D	808:809	3D	808:809	Programmed site-selective actuation can be predicted and folded into three-dimensional (3D) origami-like shapes.
32522869	1	23	theme	stimuli-responsive	122:139	arg1	properties					156:165	stimuli-responsive and deformable properties	122:165	stimuli-responsive and deformable properties	122:165	Bioinspired actuators with stimuli-responsive and deformable properties are being pursued in fields such as artificial tissues, medical devices and diagnostics, and intelligent biosensors.
32522869	1	24	theme	medical	223:229	arg1	devices					231:237	medical devices	223:237	medical devices	223:237	Bioinspired actuators with stimuli-responsive and deformable properties are being pursued in fields such as artificial tissues, medical devices and diagnostics, and intelligent biosensors.
32522869	5	25	theme	spatial	926:932	arg1	transformations					934:948	complex spatial transformations	918:948	complex spatial transformations of multilayer actuators	918:972	The reversible deformation performance of the SELP/CNF actuators was quantified, and complex spatial transformations of multilayer actuators were demonstrated, including a biomimetic flower design with selective petal movements.
32522869	2	26	theme	controlled	356:365	arg1	deformability					367:379	controlled deformability	356:379	controlled deformability	356:379	These applications require that actuator systems have biocompatibility, controlled deformability, biodegradability, mechanical durability, and stable reversibility.
32522869	3	27	theme	silk-elastin-like	548:564	arg1	hydrogels					581:589	stimuli-responsive genetically engineered silk-elastin-like protein (SELP) hydrogels	506:589	stimuli-responsive genetically engineered silk-elastin-like protein (SELP) hydrogels	506:589	Herein, we report a bionic actuator system consisting of stimuli-responsive genetically engineered silk-elastin-like protein (SELP) hydrogels and wood-derived cellulose nanofibers (CNFs), which respond to temperature and ionic strength underwater by ecofriendly methods.
32522869	2	28	contain	have	333:336	arg2	biocompatibility					338:353	biocompatibility	338:353	biocompatibility	338:353	These applications require that actuator systems have biocompatibility, controlled deformability, biodegradability, mechanical durability, and stable reversibility.
32522869	2	28	contain	have	333:336	arg2	biodegradability					382:397	biodegradability	382:397	biodegradability	382:397	These applications require that actuator systems have biocompatibility, controlled deformability, biodegradability, mechanical durability, and stable reversibility.
32522869	2	28	contain	have	333:336	arg2	durability					411:420	mechanical durability	400:420	mechanical durability	400:420	These applications require that actuator systems have biocompatibility, controlled deformability, biodegradability, mechanical durability, and stable reversibility.
32522869	2	28	contain	have	333:336	arg1	systems					325:331	actuator systems	316:331	actuator systems	316:331	These applications require that actuator systems have biocompatibility, controlled deformability, biodegradability, mechanical durability, and stable reversibility.
32522869	2	28	contain	have	333:336	arg2	deformability					367:379	controlled deformability	356:379	controlled deformability	356:379	These applications require that actuator systems have biocompatibility, controlled deformability, biodegradability, mechanical durability, and stable reversibility.
32522869	2	28	contain	have	333:336	arg2	reversibility					434:446	stable reversibility	427:446	stable reversibility	427:446	These applications require that actuator systems have biocompatibility, controlled deformability, biodegradability, mechanical durability, and stable reversibility.
32522869	3	29	link	wood-derived	595:606	arg1	CNFs					630:633	CNFs	630:633	CNFs	630:633	Herein, we report a bionic actuator system consisting of stimuli-responsive genetically engineered silk-elastin-like protein (SELP) hydrogels and wood-derived cellulose nanofibers (CNFs), which respond to temperature and ionic strength underwater by ecofriendly methods.
32522869	3	29	link	wood-derived	595:606	arg1	nanofibers					618:627	wood-derived cellulose nanofibers	595:627	wood-derived cellulose nanofibers (CNFs)	595:634	Herein, we report a bionic actuator system consisting of stimuli-responsive genetically engineered silk-elastin-like protein (SELP) hydrogels and wood-derived cellulose nanofibers (CNFs), which respond to temperature and ionic strength underwater by ecofriendly methods.
32522869	1	30	theme	deformable	145:154	arg1	properties					156:165	stimuli-responsive and deformable properties	122:165	stimuli-responsive and deformable properties	122:165	Bioinspired actuators with stimuli-responsive and deformable properties are being pursued in fields such as artificial tissues, medical devices and diagnostics, and intelligent biosensors.
32522869	3	31	theme	protein	566:572	arg1	hydrogels					581:589	stimuli-responsive genetically engineered silk-elastin-like protein (SELP) hydrogels	506:589	stimuli-responsive genetically engineered silk-elastin-like protein (SELP) hydrogels	506:589	Herein, we report a bionic actuator system consisting of stimuli-responsive genetically engineered silk-elastin-like protein (SELP) hydrogels and wood-derived cellulose nanofibers (CNFs), which respond to temperature and ionic strength underwater by ecofriendly methods.
32522869	0	32	theme	selective	55:63	arg1	behavior					85:92	selective spatial deformation behavior	55:92	selective spatial deformation behavior	55:92	Stimuli-responsive composite biopolymer actuators with selective spatial deformation behavior.
32522869	6	33	theme	biocompatible	1100:1112	arg1	materials					1132:1140	biocompatible and biodegradable materials	1100:1140	biocompatible and biodegradable materials	1100:1140	Such actuators consisting entirely of biocompatible and biodegradable materials will offer an option toward constructing stimuli-responsive systems for in vivo biomedicine soft robotics and bionic research.
32522869	5	34	theme	biomimetic	1005:1014	arg1	design					1023:1028	a biomimetic flower design	1003:1028	a biomimetic flower design with selective petal movements	1003:1059	The reversible deformation performance of the SELP/CNF actuators was quantified, and complex spatial transformations of multilayer actuators were demonstrated, including a biomimetic flower design with selective petal movements.
32522869	6	35	theme	in	1214:1215	arg1	biomedicine					1222:1232	in vivo biomedicine soft robotics and bionic research	1214:1266	biomedicine	1222:1232	Such actuators consisting entirely of biocompatible and biodegradable materials will offer an option toward constructing stimuli-responsive systems for in vivo biomedicine soft robotics and bionic research.
32522869	1	36	with	actuators	107:115	arg1	properties					156:165	stimuli-responsive and deformable properties	122:165	stimuli-responsive and deformable properties	122:165	Bioinspired actuators with stimuli-responsive and deformable properties are being pursued in fields such as artificial tissues, medical devices and diagnostics, and intelligent biosensors.
32522869	3	37	theme	actuator	476:483	arg1	system					485:490	a bionic actuator system	467:490	a bionic actuator system consisting of stimuli-responsive genetically engineered silk-elastin-like protein (SELP) hydrogels and wood-derived cellulose nanofibers (CNFs), which respond to temperature and ionic strength underwater by ecofriendly methods	467:717	Herein, we report a bionic actuator system consisting of stimuli-responsive genetically engineered silk-elastin-like protein (SELP) hydrogels and wood-derived cellulose nanofibers (CNFs), which respond to temperature and ionic strength underwater by ecofriendly methods.
32522869	3	38	theme	engineered	537:546	arg1	hydrogels					581:589	stimuli-responsive genetically engineered silk-elastin-like protein (SELP) hydrogels	506:589	stimuli-responsive genetically engineered silk-elastin-like protein (SELP) hydrogels	506:589	Herein, we report a bionic actuator system consisting of stimuli-responsive genetically engineered silk-elastin-like protein (SELP) hydrogels and wood-derived cellulose nanofibers (CNFs), which respond to temperature and ionic strength underwater by ecofriendly methods.
32522869	0	39	theme	deformation	73:83	arg1	behavior					85:92	selective spatial deformation behavior	55:92	selective spatial deformation behavior	55:92	Stimuli-responsive composite biopolymer actuators with selective spatial deformation behavior.
32522869	5	40	theme	flower	1016:1021	arg1	design					1023:1028	a biomimetic flower design	1003:1028	a biomimetic flower design with selective petal movements	1003:1059	The reversible deformation performance of the SELP/CNF actuators was quantified, and complex spatial transformations of multilayer actuators were demonstrated, including a biomimetic flower design with selective petal movements.
32522869	6	41	theme	biodegradable	1118:1130	arg1	materials					1132:1140	biocompatible and biodegradable materials	1100:1140	biocompatible and biodegradable materials	1100:1140	Such actuators consisting entirely of biocompatible and biodegradable materials will offer an option toward constructing stimuli-responsive systems for in vivo biomedicine soft robotics and bionic research.
32522869	4	42	theme	origami-like	812:823	arg1	shapes					825:830	three-dimensional (3D) origami-like shapes	789:830	three-dimensional (3D) origami-like shapes	789:830	Programmed site-selective actuation can be predicted and folded into three-dimensional (3D) origami-like shapes.
32522869	0	43	theme	spatial	65:71	arg1	behavior					85:92	selective spatial deformation behavior	55:92	selective spatial deformation behavior	55:92	Stimuli-responsive composite biopolymer actuators with selective spatial deformation behavior.
32522869	3	44	theme	ionic	670:674	arg1	strength					676:683	ionic strength	670:683	ionic strength	670:683	Herein, we report a bionic actuator system consisting of stimuli-responsive genetically engineered silk-elastin-like protein (SELP) hydrogels and wood-derived cellulose nanofibers (CNFs), which respond to temperature and ionic strength underwater by ecofriendly methods.
32522869	2	45	theme	mechanical	400:409	arg1	durability					411:420	mechanical durability	400:420	mechanical durability	400:420	These applications require that actuator systems have biocompatibility, controlled deformability, biodegradability, mechanical durability, and stable reversibility.
32522869	6	46	theme	stimuli-responsive	1183:1200	arg1	systems					1202:1208	stimuli-responsive systems	1183:1208	stimuli-responsive systems for in vivo biomedicine soft robotics and bionic research	1183:1266	Such actuators consisting entirely of biocompatible and biodegradable materials will offer an option toward constructing stimuli-responsive systems for in vivo biomedicine soft robotics and bionic research.
32522869	5	47	theme	complex	918:924	arg1	transformations					934:948	complex spatial transformations	918:948	complex spatial transformations of multilayer actuators	918:972	The reversible deformation performance of the SELP/CNF actuators was quantified, and complex spatial transformations of multilayer actuators were demonstrated, including a biomimetic flower design with selective petal movements.
32522869	6	48	theme	soft	1234:1237	arg1	robotics					1239:1246	soft robotics	1234:1246	soft robotics	1234:1246	Such actuators consisting entirely of biocompatible and biodegradable materials will offer an option toward constructing stimuli-responsive systems for in vivo biomedicine soft robotics and bionic research.
32522869	3	49	theme	SELP	575:578	arg1	hydrogels					581:589	stimuli-responsive genetically engineered silk-elastin-like protein (SELP) hydrogels	506:589	stimuli-responsive genetically engineered silk-elastin-like protein (SELP) hydrogels	506:589	Herein, we report a bionic actuator system consisting of stimuli-responsive genetically engineered silk-elastin-like protein (SELP) hydrogels and wood-derived cellulose nanofibers (CNFs), which respond to temperature and ionic strength underwater by ecofriendly methods.
32522869	1	50	theme	intelligent	260:270	arg1	biosensors					272:281	intelligent biosensors	260:281	intelligent biosensors	260:281	Bioinspired actuators with stimuli-responsive and deformable properties are being pursued in fields such as artificial tissues, medical devices and diagnostics, and intelligent biosensors.
32522869	4	51	theme	site-selective	731:744	arg1	actuation					746:754	Programmed site-selective actuation	720:754	Programmed site-selective actuation	720:754	Programmed site-selective actuation can be predicted and folded into three-dimensional (3D) origami-like shapes.
32522869	3	52	theme	cellulose	608:616	arg1	CNFs					630:633	CNFs	630:633	CNFs	630:633	Herein, we report a bionic actuator system consisting of stimuli-responsive genetically engineered silk-elastin-like protein (SELP) hydrogels and wood-derived cellulose nanofibers (CNFs), which respond to temperature and ionic strength underwater by ecofriendly methods.
32522869	3	52	theme	cellulose	608:616	arg1	nanofibers					618:627	wood-derived cellulose nanofibers	595:627	wood-derived cellulose nanofibers (CNFs)	595:634	Herein, we report a bionic actuator system consisting of stimuli-responsive genetically engineered silk-elastin-like protein (SELP) hydrogels and wood-derived cellulose nanofibers (CNFs), which respond to temperature and ionic strength underwater by ecofriendly methods.
32522869	6	53	theme	Such	1062:1065	arg1	actuators					1067:1075	Such actuators	1062:1075	Such actuators consisting entirely of biocompatible and biodegradable materials	1062:1140	Such actuators consisting entirely of biocompatible and biodegradable materials will offer an option toward constructing stimuli-responsive systems for in vivo biomedicine soft robotics and bionic research.
32522869	3	54	theme	ecofriendly	699:709	arg1	methods					711:717	ecofriendly methods	699:717	ecofriendly methods	699:717	Herein, we report a bionic actuator system consisting of stimuli-responsive genetically engineered silk-elastin-like protein (SELP) hydrogels and wood-derived cellulose nanofibers (CNFs), which respond to temperature and ionic strength underwater by ecofriendly methods.
32522869	2	55	theme	stable	427:432	arg1	reversibility					434:446	stable reversibility	427:446	stable reversibility	427:446	These applications require that actuator systems have biocompatibility, controlled deformability, biodegradability, mechanical durability, and stable reversibility.
32739340	0	0	theme	menisci	93:99	arg1	properties					38:47	the biomechanical properties	20:47	the biomechanical properties	20:47	Degeneration alters the biomechanical properties and structural composition of lateral human menisci.
32739340	0	0	theme	menisci	93:99	arg1	composition					64:74	structural composition	53:74	structural composition	53:74	Degeneration alters the biomechanical properties and structural composition of lateral human menisci.
32739340	2	1	theme	total	508:512	arg1	replacement					519:529	a total knee replacement	506:529	a total knee replacement	506:529	DESIGN Meniscal tissue from 24 patients undergoing a total knee replacement was collected and the degeneration of each region classified according to Pauli et al.
32739340	1	2	theme	degeneration	164:175	arg1	influence					151:159	the influence	147:159	the influence of degeneration on the viscoelasticity and tissue composition of human lateral menisci	147:246	OBJECTIVE Because the literature relating to the influence of degeneration on the viscoelasticity and tissue composition of human lateral menisci remains contradictory or completely lacking, the aim of this study was to fill these gaps by comprehensively characterising the biomechanical properties of menisci with regard to the degree of degeneration.
32739340	0	3	theme	human	87:91	arg1	menisci					93:99	lateral human menisci	79:99	lateral human menisci	79:99	Degeneration alters the biomechanical properties and structural composition of lateral human menisci.
32739340	6	4	theme	collagen	1257:1264	arg1	content					1266:1272	collagen content	1257:1272	collagen content	1257:1272	However, the tensile modulus displayed a tendency to decrease with increasing degeneration, which might be due to the significantly decreasing amount of collagen content identified by the IR measurements.
32739340	7	5	theme	degenerative	1429:1440	arg1	processes					1442:1450	degenerative processes	1429:1450	degenerative processes	1429:1450	CONCLUSION The findings of the current study may contribute to the understanding of meniscus degeneration, showing that degenerative processes appear to mainly worsen viscoelastic properties of the inner circumference by disrupting the collagen integrity.
32739340	4	6	from	changes	800:806	arg1	composition					826:836	the structural composition	811:836	the structural composition	811:836	Additionally, the water content was determined and infrared (IR) spectroscopy was applied to detect changes in the structural composition, particularly of the proteoglycan and collagen content.
32739340	6	7	theme	decreasing	1236:1245	arg1	amount					1247:1252	the significantly decreasing amount	1218:1252	the significantly decreasing amount of collagen content identified by the IR measurements	1218:1306	However, the tensile modulus displayed a tendency to decrease with increasing degeneration, which might be due to the significantly decreasing amount of collagen content identified by the IR measurements.
32739340	6	7	theme	decreasing	1236:1245	arg1	content					1266:1272	collagen content	1257:1272	collagen content	1257:1272	However, the tensile modulus displayed a tendency to decrease with increasing degeneration, which might be due to the significantly decreasing amount of collagen content identified by the IR measurements.
32739340	7	8	theme	circumference	1513:1525	arg1	properties					1489:1498	viscoelastic properties	1476:1498	viscoelastic properties of the inner circumference	1476:1525	CONCLUSION The findings of the current study may contribute to the understanding of meniscus degeneration, showing that degenerative processes appear to mainly worsen viscoelastic properties of the inner circumference by disrupting the collagen integrity.
32739340	1	9	from	influence	151:159	arg1	viscoelasticity					184:198	viscoelasticity	184:198	viscoelasticity	184:198	OBJECTIVE Because the literature relating to the influence of degeneration on the viscoelasticity and tissue composition of human lateral menisci remains contradictory or completely lacking, the aim of this study was to fill these gaps by comprehensively characterising the biomechanical properties of menisci with regard to the degree of degeneration.
32739340	1	9	from	influence	151:159	arg1	composition					211:221	tissue composition	204:221	tissue composition	204:221	OBJECTIVE Because the literature relating to the influence of degeneration on the viscoelasticity and tissue composition of human lateral menisci remains contradictory or completely lacking, the aim of this study was to fill these gaps by comprehensively characterising the biomechanical properties of menisci with regard to the degree of degeneration.
32739340	5	10	theme	degeneration	931:942	arg1	degree					921:926	an increasing degree	907:926	an increasing degree of degeneration, a significant decrease of the equilibrium modulus	907:993	RESULTS With an increasing degree of degeneration, a significant decrease of the equilibrium modulus was detected, while simultaneously the water content and the hydraulic permeability significantly increased.
32739340	1	11	theme	biomechanical	376:388	arg1	properties					390:399	the biomechanical properties	372:399	the biomechanical properties of menisci	372:410	OBJECTIVE Because the literature relating to the influence of degeneration on the viscoelasticity and tissue composition of human lateral menisci remains contradictory or completely lacking, the aim of this study was to fill these gaps by comprehensively characterising the biomechanical properties of menisci with regard to the degree of degeneration.
32739340	4	12	theme	collagen	876:883	arg1	content					885:891	the proteoglycan and collagen content	855:891	content	885:891	Additionally, the water content was determined and infrared (IR) spectroscopy was applied to detect changes in the structural composition, particularly of the proteoglycan and collagen content.
32739340	7	13	theme	meniscus	1393:1400	arg1	degeneration					1402:1413	meniscus degeneration	1393:1413	meniscus degeneration	1393:1413	CONCLUSION The findings of the current study may contribute to the understanding of meniscus degeneration, showing that degenerative processes appear to mainly worsen viscoelastic properties of the inner circumference by disrupting the collagen integrity.
32739340	5	14	theme	hydraulic	1056:1064	arg1	permeability					1066:1077	the hydraulic permeability	1052:1077	the hydraulic permeability	1052:1077	RESULTS With an increasing degree of degeneration, a significant decrease of the equilibrium modulus was detected, while simultaneously the water content and the hydraulic permeability significantly increased.
32739340	2	15	from	patients	486:493	arg1	tissue					471:476	DESIGN Meniscal tissue	455:476	DESIGN Meniscal tissue from 24 patients undergoing a total knee replacement	455:529	DESIGN Meniscal tissue from 24 patients undergoing a total knee replacement was collected and the degeneration of each region classified according to Pauli et al.
32739340	7	16	theme	viscoelastic	1476:1487	arg1	properties					1489:1498	viscoelastic properties	1476:1498	viscoelastic properties of the inner circumference	1476:1525	CONCLUSION The findings of the current study may contribute to the understanding of meniscus degeneration, showing that degenerative processes appear to mainly worsen viscoelastic properties of the inner circumference by disrupting the collagen integrity.
32739340	7	17	theme	collagen	1545:1552	arg1	integrity					1554:1562	the collagen integrity	1541:1562	the collagen integrity	1541:1562	CONCLUSION The findings of the current study may contribute to the understanding of meniscus degeneration, showing that degenerative processes appear to mainly worsen viscoelastic properties of the inner circumference by disrupting the collagen integrity.
32739340	6	18	theme	tensile	1117:1123	arg1	modulus					1125:1131	the tensile modulus	1113:1131	the tensile modulus	1113:1131	However, the tensile modulus displayed a tendency to decrease with increasing degeneration, which might be due to the significantly decreasing amount of collagen content identified by the IR measurements.
32739340	2	19	theme	Meniscal	462:469	arg1	tissue					471:476	DESIGN Meniscal tissue	455:476	DESIGN Meniscal tissue from 24 patients undergoing a total knee replacement	455:529	DESIGN Meniscal tissue from 24 patients undergoing a total knee replacement was collected and the degeneration of each region classified according to Pauli et al.
32739340	7	20	theme	inner	1507:1511	arg1	circumference					1513:1525	the inner circumference	1503:1525	the inner circumference	1503:1525	CONCLUSION The findings of the current study may contribute to the understanding of meniscus degeneration, showing that degenerative processes appear to mainly worsen viscoelastic properties of the inner circumference by disrupting the collagen integrity.
32739340	1	21	theme	degeneration	441:452	arg1	degree					431:436	the degree	427:436	the degree of degeneration	427:452	OBJECTIVE Because the literature relating to the influence of degeneration on the viscoelasticity and tissue composition of human lateral menisci remains contradictory or completely lacking, the aim of this study was to fill these gaps by comprehensively characterising the biomechanical properties of menisci with regard to the degree of degeneration.
32739340	5	22	theme	equilibrium	975:985	arg1	modulus					987:993	the equilibrium modulus	971:993	the equilibrium modulus	971:993	RESULTS With an increasing degree of degeneration, a significant decrease of the equilibrium modulus was detected, while simultaneously the water content and the hydraulic permeability significantly increased.
32739340	1	23	theme	tissue	204:209	arg1	composition					211:221	tissue composition	204:221	tissue composition	204:221	OBJECTIVE Because the literature relating to the influence of degeneration on the viscoelasticity and tissue composition of human lateral menisci remains contradictory or completely lacking, the aim of this study was to fill these gaps by comprehensively characterising the biomechanical properties of menisci with regard to the degree of degeneration.
32739340	0	24	theme	biomechanical	24:36	arg1	properties					38:47	the biomechanical properties	20:47	the biomechanical properties	20:47	Degeneration alters the biomechanical properties and structural composition of lateral human menisci.
32739340	2	25	theme	region	574:579	arg1	degeneration					553:564	the degeneration	549:564	the degeneration of each region	549:579	DESIGN Meniscal tissue from 24 patients undergoing a total knee replacement was collected and the degeneration of each region classified according to Pauli et al.
32739340	5	26	theme	modulus	987:993	arg1	degeneration					931:942	degeneration	931:942	degeneration	931:942	RESULTS With an increasing degree of degeneration, a significant decrease of the equilibrium modulus was detected, while simultaneously the water content and the hydraulic permeability significantly increased.
32739340	5	26	theme	modulus	987:993	arg1	decrease					959:966	a significant decrease	945:966	a significant decrease of the equilibrium modulus	945:993	RESULTS With an increasing degree of degeneration, a significant decrease of the equilibrium modulus was detected, while simultaneously the water content and the hydraulic permeability significantly increased.
32739340	1	27	theme	menisci	404:410	arg1	properties					390:399	the biomechanical properties	372:399	the biomechanical properties of menisci	372:410	OBJECTIVE Because the literature relating to the influence of degeneration on the viscoelasticity and tissue composition of human lateral menisci remains contradictory or completely lacking, the aim of this study was to fill these gaps by comprehensively characterising the biomechanical properties of menisci with regard to the degree of degeneration.
32739340	1	28	theme	study	309:313	arg1	aim					297:299	the aim	293:299	the aim of this study	293:313	OBJECTIVE Because the literature relating to the influence of degeneration on the viscoelasticity and tissue composition of human lateral menisci remains contradictory or completely lacking, the aim of this study was to fill these gaps by comprehensively characterising the biomechanical properties of menisci with regard to the degree of degeneration.
32739340	7	29	theme	study	1348:1352	arg1	findings					1324:1331	The findings	1320:1331	The findings of the current study	1320:1352	CONCLUSION The findings of the current study may contribute to the understanding of meniscus degeneration, showing that degenerative processes appear to mainly worsen viscoelastic properties of the inner circumference by disrupting the collagen integrity.
32739340	6	30	theme	IR	1292:1293	arg1	measurements					1295:1306	the IR measurements	1288:1306	the IR measurements	1288:1306	However, the tensile modulus displayed a tendency to decrease with increasing degeneration, which might be due to the significantly decreasing amount of collagen content identified by the IR measurements.
32739340	5	31	theme	increasing	910:919	arg1	degree					921:926	an increasing degree	907:926	an increasing degree of degeneration, a significant decrease of the equilibrium modulus	907:993	RESULTS With an increasing degree of degeneration, a significant decrease of the equilibrium modulus was detected, while simultaneously the water content and the hydraulic permeability significantly increased.
32739340	1	32	theme	human	226:230	arg1	menisci					240:246	human lateral menisci	226:246	human lateral menisci	226:246	OBJECTIVE Because the literature relating to the influence of degeneration on the viscoelasticity and tissue composition of human lateral menisci remains contradictory or completely lacking, the aim of this study was to fill these gaps by comprehensively characterising the biomechanical properties of menisci with regard to the degree of degeneration.
32739340	3	33	theme	tensile	670:676	arg1	tests					678:682	tensile tests	670:682	tensile tests	670:682	For biomechanical characterisation, compression and tensile tests were performed.
32739340	2	34	theme	DESIGN	455:460	arg1	tissue					471:476	DESIGN Meniscal tissue	455:476	DESIGN Meniscal tissue from 24 patients undergoing a total knee replacement	455:529	DESIGN Meniscal tissue from 24 patients undergoing a total knee replacement was collected and the degeneration of each region classified according to Pauli et al.
32739340	1	35	theme	lateral	232:238	arg1	menisci					240:246	human lateral menisci	226:246	human lateral menisci	226:246	OBJECTIVE Because the literature relating to the influence of degeneration on the viscoelasticity and tissue composition of human lateral menisci remains contradictory or completely lacking, the aim of this study was to fill these gaps by comprehensively characterising the biomechanical properties of menisci with regard to the degree of degeneration.
32739340	2	36	theme	Pauli	605:609	arg1	et al					611:615	Pauli et al	605:615	Pauli et al	605:615	DESIGN Meniscal tissue from 24 patients undergoing a total knee replacement was collected and the degeneration of each region classified according to Pauli et al.
32739340	4	37	theme	proteoglycan	859:870	arg1	content					885:891	the proteoglycan and collagen content	855:891	content	885:891	Additionally, the water content was determined and infrared (IR) spectroscopy was applied to detect changes in the structural composition, particularly of the proteoglycan and collagen content.
32739340	4	38	theme	content	885:891	arg1	composition					826:836	the structural composition	811:836	the structural composition	811:836	Additionally, the water content was determined and infrared (IR) spectroscopy was applied to detect changes in the structural composition, particularly of the proteoglycan and collagen content.
32739340	4	39	theme	infrared	751:758	arg1	spectroscopy					765:776	infrared (IR) spectroscopy	751:776	infrared (IR) spectroscopy	751:776	Additionally, the water content was determined and infrared (IR) spectroscopy was applied to detect changes in the structural composition, particularly of the proteoglycan and collagen content.
32739340	0	40	theme	structural	53:62	arg1	composition					64:74	structural composition	53:74	structural composition	53:74	Degeneration alters the biomechanical properties and structural composition of lateral human menisci.
32739340	1	41	dep	viscoelasticity	184:198	arg1	the					180:182	the	180:182	the	180:182	OBJECTIVE Because the literature relating to the influence of degeneration on the viscoelasticity and tissue composition of human lateral menisci remains contradictory or completely lacking, the aim of this study was to fill these gaps by comprehensively characterising the biomechanical properties of menisci with regard to the degree of degeneration.
32739340	5	42	theme	significant	947:957	arg1	degeneration					931:942	degeneration	931:942	degeneration	931:942	RESULTS With an increasing degree of degeneration, a significant decrease of the equilibrium modulus was detected, while simultaneously the water content and the hydraulic permeability significantly increased.
32739340	5	42	theme	significant	947:957	arg1	decrease					959:966	a significant decrease	945:966	a significant decrease of the equilibrium modulus	945:993	RESULTS With an increasing degree of degeneration, a significant decrease of the equilibrium modulus was detected, while simultaneously the water content and the hydraulic permeability significantly increased.
32739340	5	43	with	RESULTS	894:900	arg1	degree					921:926	an increasing degree	907:926	an increasing degree of degeneration, a significant decrease of the equilibrium modulus	907:993	RESULTS With an increasing degree of degeneration, a significant decrease of the equilibrium modulus was detected, while simultaneously the water content and the hydraulic permeability significantly increased.
32739340	6	44	theme	content	1266:1272	arg1	amount					1247:1252	the significantly decreasing amount	1218:1252	the significantly decreasing amount of collagen content identified by the IR measurements	1218:1306	However, the tensile modulus displayed a tendency to decrease with increasing degeneration, which might be due to the significantly decreasing amount of collagen content identified by the IR measurements.
32739340	6	44	theme	content	1266:1272	arg1	content					1266:1272	collagen content	1257:1272	collagen content	1257:1272	However, the tensile modulus displayed a tendency to decrease with increasing degeneration, which might be due to the significantly decreasing amount of collagen content identified by the IR measurements.
32739340	4	45	theme	structural	815:824	arg1	composition					826:836	the structural composition	811:836	the structural composition	811:836	Additionally, the water content was determined and infrared (IR) spectroscopy was applied to detect changes in the structural composition, particularly of the proteoglycan and collagen content.
32739340	3	46	theme	biomechanical	622:634	arg1	characterisation					636:651	biomechanical characterisation	622:651	biomechanical characterisation	622:651	For biomechanical characterisation, compression and tensile tests were performed.
32739340	7	47	dep	CONCLUSION	1309:1318	arg1	contribute					1358:1367	contribute	1358:1367	contribute	1358:1367	CONCLUSION The findings of the current study may contribute to the understanding of meniscus degeneration, showing that degenerative processes appear to mainly worsen viscoelastic properties of the inner circumference by disrupting the collagen integrity.
32739340	1	48	theme	menisci	240:246	arg1	viscoelasticity					184:198	viscoelasticity	184:198	viscoelasticity	184:198	OBJECTIVE Because the literature relating to the influence of degeneration on the viscoelasticity and tissue composition of human lateral menisci remains contradictory or completely lacking, the aim of this study was to fill these gaps by comprehensively characterising the biomechanical properties of menisci with regard to the degree of degeneration.
32739340	1	48	theme	menisci	240:246	arg1	composition					211:221	tissue composition	204:221	tissue composition	204:221	OBJECTIVE Because the literature relating to the influence of degeneration on the viscoelasticity and tissue composition of human lateral menisci remains contradictory or completely lacking, the aim of this study was to fill these gaps by comprehensively characterising the biomechanical properties of menisci with regard to the degree of degeneration.
32739340	5	49	theme	water	1034:1038	arg1	content					1040:1046	simultaneously the water content	1015:1046	simultaneously the water content	1015:1046	RESULTS With an increasing degree of degeneration, a significant decrease of the equilibrium modulus was detected, while simultaneously the water content and the hydraulic permeability significantly increased.
32739340	0	50	theme	lateral	79:85	arg1	menisci					93:99	lateral human menisci	79:99	lateral human menisci	79:99	Degeneration alters the biomechanical properties and structural composition of lateral human menisci.
32739340	1	51	dep	lacking	284:290	arg1	contradictory					256:268	contradictory	256:268	contradictory	256:268	OBJECTIVE Because the literature relating to the influence of degeneration on the viscoelasticity and tissue composition of human lateral menisci remains contradictory or completely lacking, the aim of this study was to fill these gaps by comprehensively characterising the biomechanical properties of menisci with regard to the degree of degeneration.
32739340	7	52	theme	degeneration	1402:1413	arg1	understanding					1376:1388	the understanding	1372:1388	the understanding of meniscus degeneration	1372:1413	CONCLUSION The findings of the current study may contribute to the understanding of meniscus degeneration, showing that degenerative processes appear to mainly worsen viscoelastic properties of the inner circumference by disrupting the collagen integrity.
32739340	2	53	theme	knee	514:517	arg1	replacement					519:529	a total knee replacement	506:529	a total knee replacement	506:529	DESIGN Meniscal tissue from 24 patients undergoing a total knee replacement was collected and the degeneration of each region classified according to Pauli et al.
32739340	7	54	theme	current	1340:1346	arg1	study					1348:1352	the current study	1336:1352	the current study	1336:1352	CONCLUSION The findings of the current study may contribute to the understanding of meniscus degeneration, showing that degenerative processes appear to mainly worsen viscoelastic properties of the inner circumference by disrupting the collagen integrity.
32739340	4	55	theme	water	718:722	arg1	content					724:730	the water content	714:730	the water content	714:730	Additionally, the water content was determined and infrared (IR) spectroscopy was applied to detect changes in the structural composition, particularly of the proteoglycan and collagen content.
34943920	0	0	theme	GMP	94:96	arg1	Applications					104:115	GMP Grade Applications	94:115	GMP Grade Applications	94:115	Nucleofection of Adipose Mesenchymal Stem/Stromal Cells: Improved Transfection Efficiency for GMP Grade Applications.
34943920	8	1	theme	cell	1229:1232	arg1	conditions					1242:1251	tuning clinical-grade compatible cell culture conditions	1196:1251	tuning clinical-grade compatible cell culture conditions	1196:1251	In conclusion, we reported for the first time that tuning clinical-grade compatible cell culture conditions can significantly improve ASC transfection efficiency by a non-viral and safe approach.
34943920	2	2	theme	clinical	349:356	arg1	settings					358:365	clinical settings	349:365	clinical settings	349:365	Such a technique is useful to improve therapeutic effectiveness of adipose tissue mesenchymal stem cells (ASC) in clinical settings, but improvement of NF efficiency is mandatory.
34943920	7	3	theme	statistical	880:890	arg1	correlation					892:902	A statistical correlation	878:902	A statistical correlation between NF efficiency and lipid raft availability on cell membranes	878:970	A statistical correlation between NF efficiency and lipid raft availability on cell membranes was shown, even though a direct relationship could not be demonstrated: attempts to selectively modulate lipid rafts levels were, in fact, limited by technical constraints.
34943920	4	4	theme	post-transfection	565:581	arg1	viability					583:591	post-transfection viability	565:591	post-transfection viability	565:591	We showed a dramatically increased NF efficiency and post-transfection viability in ASC expanded in presence of SRGF (vs. fetal bovine serum).
34943920	6	5	theme	raft	813:816	arg1	occurrence					818:827	membrane lipid raft occurrence	798:827	membrane lipid raft occurrence	798:827	SRGF increased n-6/n-3 ratio, reduced membrane lipid raft occurrence, and lowered intracellular actin content in ASC.
34943920	9	6	theme	ASC	1543:1545	arg1	efficiency					1550:1559	ASC NF efficiency	1543:1559	ASC NF efficiency	1543:1559	A deep mechanistic characterization is extremely complex, but we can hypothesize that integrated changes in membrane structure and intracellular actin content could contribute to explain SRGF impact on ASC NF efficiency.
34943920	8	7	theme	compatible	1218:1227	arg1	conditions					1242:1251	tuning clinical-grade compatible cell culture conditions	1196:1251	tuning clinical-grade compatible cell culture conditions	1196:1251	In conclusion, we reported for the first time that tuning clinical-grade compatible cell culture conditions can significantly improve ASC transfection efficiency by a non-viral and safe approach.
34943920	5	8	theme	vesicle	705:711	arg1	endocytosis					713:723	increased vesicle endocytosis	695:723	increased vesicle endocytosis	695:723	SRGF expanded ASC were characterized by increased vesicle endocytosis but lower phagocytosis properties.
34943920	6	9	theme	lipid	807:811	arg1	occurrence					818:827	membrane lipid raft occurrence	798:827	membrane lipid raft occurrence	798:827	SRGF increased n-6/n-3 ratio, reduced membrane lipid raft occurrence, and lowered intracellular actin content in ASC.
34943920	9	10	theme	NF	1547:1548	arg1	efficiency					1550:1559	ASC NF efficiency	1543:1559	ASC NF efficiency	1543:1559	A deep mechanistic characterization is extremely complex, but we can hypothesize that integrated changes in membrane structure and intracellular actin content could contribute to explain SRGF impact on ASC NF efficiency.
34943920	2	11	theme	adipose	302:308	arg1	tissue					310:315	adipose tissue mesenchymal stem cells (ASC)	302:344	adipose tissue mesenchymal stem cells (ASC)	302:344	Such a technique is useful to improve therapeutic effectiveness of adipose tissue mesenchymal stem cells (ASC) in clinical settings, but improvement of NF efficiency is mandatory.
34943920	0	12	theme	Grade	98:102	arg1	Applications					104:115	GMP Grade Applications	94:115	GMP Grade Applications	94:115	Nucleofection of Adipose Mesenchymal Stem/Stromal Cells: Improved Transfection Efficiency for GMP Grade Applications.
34943920	6	13	theme	membrane	798:805	arg1	occurrence					818:827	membrane lipid raft occurrence	798:827	membrane lipid raft occurrence	798:827	SRGF increased n-6/n-3 ratio, reduced membrane lipid raft occurrence, and lowered intracellular actin content in ASC.
34943920	3	14	theme	additive	484:491	arg1	Supernatant					415:425	Supernatant	415:425	Supernatant rich in growth factors (SRGF)	415:455	Supernatant rich in growth factors (SRGF) is a clinical-grade medium additive for ASC expansion.
34943920	3	14	theme	additive	484:491	arg1	medium					477:482	a clinical-grade medium	460:482	a clinical-grade medium additive for ASC expansion	460:509	Supernatant rich in growth factors (SRGF) is a clinical-grade medium additive for ASC expansion.
34943920	9	15	theme	integrated	1427:1436	arg1	changes					1438:1444	integrated changes	1427:1444	integrated changes in membrane structure and intracellular actin content	1427:1498	A deep mechanistic characterization is extremely complex, but we can hypothesize that integrated changes in membrane structure and intracellular actin content could contribute to explain SRGF impact on ASC NF efficiency.
34943920	3	16	theme	clinical-grade	462:475	arg1	Supernatant					415:425	Supernatant	415:425	Supernatant rich in growth factors (SRGF)	415:455	Supernatant rich in growth factors (SRGF) is a clinical-grade medium additive for ASC expansion.
34943920	3	16	theme	clinical-grade	462:475	arg1	medium					477:482	a clinical-grade medium	460:482	a clinical-grade medium additive for ASC expansion	460:509	Supernatant rich in growth factors (SRGF) is a clinical-grade medium additive for ASC expansion.
34943920	7	17	from	efficiency	915:924	arg1	membranes					962:970	cell membranes	957:970	cell membranes	957:970	A statistical correlation between NF efficiency and lipid raft availability on cell membranes was shown, even though a direct relationship could not be demonstrated: attempts to selectively modulate lipid rafts levels were, in fact, limited by technical constraints.
34943920	7	18	theme	cell	957:960	arg1	membranes					962:970	cell membranes	957:970	cell membranes	957:970	A statistical correlation between NF efficiency and lipid raft availability on cell membranes was shown, even though a direct relationship could not be demonstrated: attempts to selectively modulate lipid rafts levels were, in fact, limited by technical constraints.
34943920	2	19	theme	therapeutic	273:283	arg1	effectiveness					285:297	therapeutic effectiveness	273:297	therapeutic effectiveness of adipose tissue mesenchymal stem cells (ASC) in clinical settings	273:365	Such a technique is useful to improve therapeutic effectiveness of adipose tissue mesenchymal stem cells (ASC) in clinical settings, but improvement of NF efficiency is mandatory.
34943920	9	20	theme	membrane	1449:1456	arg1	structure					1458:1466	membrane structure	1449:1466	membrane structure	1449:1466	A deep mechanistic characterization is extremely complex, but we can hypothesize that integrated changes in membrane structure and intracellular actin content could contribute to explain SRGF impact on ASC NF efficiency.
34943920	1	21	theme	transfection	158:169	arg1	method					171:176	a safe, non-viral transfection method	140:176	a safe, non-viral transfection method	140:176	Nucleofection (NF) is a safe, non-viral transfection method, compatible with Good Manufacturing Practice guidelines.
34943920	1	21	theme	transfection	158:169	arg1	Nucleofection					118:130	Nucleofection	118:130	Nucleofection (NF)	118:135	Nucleofection (NF) is a safe, non-viral transfection method, compatible with Good Manufacturing Practice guidelines.
34943920	5	22	theme	lower	729:733	arg1	phagocytosis					735:746	lower phagocytosis	729:746	lower phagocytosis	729:746	SRGF expanded ASC were characterized by increased vesicle endocytosis but lower phagocytosis properties.
34943920	7	23	theme	technical	1122:1130	arg1	constraints					1132:1142	technical constraints	1122:1142	technical constraints	1122:1142	A statistical correlation between NF efficiency and lipid raft availability on cell membranes was shown, even though a direct relationship could not be demonstrated: attempts to selectively modulate lipid rafts levels were, in fact, limited by technical constraints.
34943920	7	24	theme	raft	936:939	arg1	availability					941:952	lipid raft availability	930:952	lipid raft availability	930:952	A statistical correlation between NF efficiency and lipid raft availability on cell membranes was shown, even though a direct relationship could not be demonstrated: attempts to selectively modulate lipid rafts levels were, in fact, limited by technical constraints.
34943920	3	25	theme	growth	435:440	arg1	SRGF					451:454	SRGF	451:454	SRGF	451:454	Supernatant rich in growth factors (SRGF) is a clinical-grade medium additive for ASC expansion.
34943920	3	25	theme	growth	435:440	arg1	factors					442:448	growth factors	435:448	growth factors (SRGF)	435:455	Supernatant rich in growth factors (SRGF) is a clinical-grade medium additive for ASC expansion.
34943920	9	26	theme	deep	1343:1346	arg1	characterization					1360:1375	A deep mechanistic characterization	1341:1375	A deep mechanistic characterization	1341:1375	A deep mechanistic characterization is extremely complex, but we can hypothesize that integrated changes in membrane structure and intracellular actin content could contribute to explain SRGF impact on ASC NF efficiency.
34943920	9	26	theme	deep	1343:1346	arg1	complex					1390:1396	complex	1390:1396	complex	1390:1396	A deep mechanistic characterization is extremely complex, but we can hypothesize that integrated changes in membrane structure and intracellular actin content could contribute to explain SRGF impact on ASC NF efficiency.
34943920	7	27	theme	lipid	1077:1081	arg1	rafts					1083:1087	lipid rafts	1077:1087	lipid rafts levels were, in fact, limited by technical constraints	1077:1142	A statistical correlation between NF efficiency and lipid raft availability on cell membranes was shown, even though a direct relationship could not be demonstrated: attempts to selectively modulate lipid rafts levels were, in fact, limited by technical constraints.
34943920	4	28	theme	fetal	634:638	arg1	serum					647:651	fetal bovine serum	634:651	fetal bovine serum	634:651	We showed a dramatically increased NF efficiency and post-transfection viability in ASC expanded in presence of SRGF (vs. fetal bovine serum).
34943920	4	28	theme	fetal	634:638	arg1	SRGF					624:627	SRGF	624:627	SRGF (vs. fetal bovine serum)	624:652	We showed a dramatically increased NF efficiency and post-transfection viability in ASC expanded in presence of SRGF (vs. fetal bovine serum).
34943920	2	29	theme	stem	329:332	arg1	ASC					341:343	ASC	341:343	ASC	341:343	Such a technique is useful to improve therapeutic effectiveness of adipose tissue mesenchymal stem cells (ASC) in clinical settings, but improvement of NF efficiency is mandatory.
34943920	2	29	theme	stem	329:332	arg1	cells					334:338	mesenchymal stem cells	317:338	adipose tissue mesenchymal stem cells (ASC)	302:344	Such a technique is useful to improve therapeutic effectiveness of adipose tissue mesenchymal stem cells (ASC) in clinical settings, but improvement of NF efficiency is mandatory.
34943920	5	30	theme	SRGF	655:658	arg1	ASC					669:671	SRGF expanded ASC	655:671	SRGF expanded ASC	655:671	SRGF expanded ASC were characterized by increased vesicle endocytosis but lower phagocytosis properties.
34943920	8	31	theme	culture	1234:1240	arg1	conditions					1242:1251	tuning clinical-grade compatible cell culture conditions	1196:1251	tuning clinical-grade compatible cell culture conditions	1196:1251	In conclusion, we reported for the first time that tuning clinical-grade compatible cell culture conditions can significantly improve ASC transfection efficiency by a non-viral and safe approach.
34943920	4	32	theme	increased	537:545	arg1	efficiency					550:559	a dramatically increased NF efficiency	522:559	a dramatically increased NF efficiency	522:559	We showed a dramatically increased NF efficiency and post-transfection viability in ASC expanded in presence of SRGF (vs. fetal bovine serum).
34943920	8	33	theme	transfection	1283:1294	arg1	efficiency					1296:1305	ASC transfection efficiency	1279:1305	ASC transfection efficiency	1279:1305	In conclusion, we reported for the first time that tuning clinical-grade compatible cell culture conditions can significantly improve ASC transfection efficiency by a non-viral and safe approach.
34943920	4	34	theme	bovine	640:645	arg1	serum					647:651	fetal bovine serum	634:651	fetal bovine serum	634:651	We showed a dramatically increased NF efficiency and post-transfection viability in ASC expanded in presence of SRGF (vs. fetal bovine serum).
34943920	4	34	theme	bovine	640:645	arg1	SRGF					624:627	SRGF	624:627	SRGF (vs. fetal bovine serum)	624:652	We showed a dramatically increased NF efficiency and post-transfection viability in ASC expanded in presence of SRGF (vs. fetal bovine serum).
34943920	2	35	theme	mesenchymal	317:327	arg1	ASC					341:343	ASC	341:343	ASC	341:343	Such a technique is useful to improve therapeutic effectiveness of adipose tissue mesenchymal stem cells (ASC) in clinical settings, but improvement of NF efficiency is mandatory.
34943920	2	35	theme	mesenchymal	317:327	arg1	cells					334:338	mesenchymal stem cells	317:338	adipose tissue mesenchymal stem cells (ASC)	302:344	Such a technique is useful to improve therapeutic effectiveness of adipose tissue mesenchymal stem cells (ASC) in clinical settings, but improvement of NF efficiency is mandatory.
34943920	1	36	theme	compatible	179:188	arg1	method					171:176	a safe, non-viral transfection method	140:176	a safe, non-viral transfection method	140:176	Nucleofection (NF) is a safe, non-viral transfection method, compatible with Good Manufacturing Practice guidelines.
34943920	1	36	theme	compatible	179:188	arg1	Nucleofection					118:130	Nucleofection	118:130	Nucleofection (NF)	118:135	Nucleofection (NF) is a safe, non-viral transfection method, compatible with Good Manufacturing Practice guidelines.
34943920	0	37	theme	Mesenchymal	25:35	arg1	Cells					50:54	Adipose Mesenchymal Stem/Stromal Cells	17:54	Adipose Mesenchymal Stem/Stromal Cells	17:54	Nucleofection of Adipose Mesenchymal Stem/Stromal Cells: Improved Transfection Efficiency for GMP Grade Applications.
34943920	6	38	theme	n-6/n-3	775:781	arg1	ratio					783:787	n-6/n-3 ratio	775:787	n-6/n-3 ratio	775:787	SRGF increased n-6/n-3 ratio, reduced membrane lipid raft occurrence, and lowered intracellular actin content in ASC.
34943920	7	39	theme	lipid	930:934	arg1	availability					941:952	lipid raft availability	930:952	lipid raft availability	930:952	A statistical correlation between NF efficiency and lipid raft availability on cell membranes was shown, even though a direct relationship could not be demonstrated: attempts to selectively modulate lipid rafts levels were, in fact, limited by technical constraints.
34943920	2	40	theme	tissue	310:315	arg1	effectiveness					285:297	therapeutic effectiveness	273:297	therapeutic effectiveness of adipose tissue mesenchymal stem cells (ASC) in clinical settings	273:365	Such a technique is useful to improve therapeutic effectiveness of adipose tissue mesenchymal stem cells (ASC) in clinical settings, but improvement of NF efficiency is mandatory.
34943920	4	41	theme	SRGF	624:627	arg1	presence					612:619	presence	612:619	presence of SRGF (vs. fetal bovine serum)	612:652	We showed a dramatically increased NF efficiency and post-transfection viability in ASC expanded in presence of SRGF (vs. fetal bovine serum).
34943920	0	42	theme	Adipose	17:23	arg1	Cells					50:54	Adipose Mesenchymal Stem/Stromal Cells	17:54	Adipose Mesenchymal Stem/Stromal Cells	17:54	Nucleofection of Adipose Mesenchymal Stem/Stromal Cells: Improved Transfection Efficiency for GMP Grade Applications.
34943920	9	43	theme	SRGF	1528:1531	arg1	impact					1533:1538	SRGF impact	1528:1538	SRGF impact on ASC NF efficiency	1528:1559	A deep mechanistic characterization is extremely complex, but we can hypothesize that integrated changes in membrane structure and intracellular actin content could contribute to explain SRGF impact on ASC NF efficiency.
34943920	1	44	dep	safe	142:145	arg1	non-viral					148:156	non-viral	148:156	non-viral	148:156	Nucleofection (NF) is a safe, non-viral transfection method, compatible with Good Manufacturing Practice guidelines.
34943920	1	45	theme	Good	195:198	arg1	guidelines					223:232	Good Manufacturing Practice guidelines	195:232	Good Manufacturing Practice guidelines	195:232	Nucleofection (NF) is a safe, non-viral transfection method, compatible with Good Manufacturing Practice guidelines.
34943920	0	46	theme	Cells	50:54	arg1	Nucleofection					0:12	Nucleofection	0:12	Nucleofection of Adipose Mesenchymal Stem/Stromal Cells: Improved Transfection Efficiency for GMP Grade Applications.	0:116	Nucleofection of Adipose Mesenchymal Stem/Stromal Cells: Improved Transfection Efficiency for GMP Grade Applications.
34943920	4	47	theme	NF	547:548	arg1	efficiency					550:559	a dramatically increased NF efficiency	522:559	a dramatically increased NF efficiency	522:559	We showed a dramatically increased NF efficiency and post-transfection viability in ASC expanded in presence of SRGF (vs. fetal bovine serum).
34943920	9	48	from	impact	1533:1538	arg1	efficiency					1550:1559	ASC NF efficiency	1543:1559	ASC NF efficiency	1543:1559	A deep mechanistic characterization is extremely complex, but we can hypothesize that integrated changes in membrane structure and intracellular actin content could contribute to explain SRGF impact on ASC NF efficiency.
34943920	9	49	theme	actin	1486:1490	arg1	content					1492:1498	intracellular actin content	1472:1498	intracellular actin content	1472:1498	A deep mechanistic characterization is extremely complex, but we can hypothesize that integrated changes in membrane structure and intracellular actin content could contribute to explain SRGF impact on ASC NF efficiency.
34943920	3	50	theme	rich	427:430	arg1	Supernatant					415:425	Supernatant	415:425	Supernatant rich in growth factors (SRGF)	415:455	Supernatant rich in growth factors (SRGF) is a clinical-grade medium additive for ASC expansion.
34943920	3	50	theme	rich	427:430	arg1	medium					477:482	a clinical-grade medium	460:482	a clinical-grade medium additive for ASC expansion	460:509	Supernatant rich in growth factors (SRGF) is a clinical-grade medium additive for ASC expansion.
34943920	1	51	theme	Manufacturing	200:212	arg1	guidelines					223:232	Good Manufacturing Practice guidelines	195:232	Good Manufacturing Practice guidelines	195:232	Nucleofection (NF) is a safe, non-viral transfection method, compatible with Good Manufacturing Practice guidelines.
34943920	2	52	theme	NF	387:388	arg1	efficiency					390:399	NF efficiency	387:399	NF efficiency	387:399	Such a technique is useful to improve therapeutic effectiveness of adipose tissue mesenchymal stem cells (ASC) in clinical settings, but improvement of NF efficiency is mandatory.
34943920	9	53	theme	mechanistic	1348:1358	arg1	characterization					1360:1375	A deep mechanistic characterization	1341:1375	A deep mechanistic characterization	1341:1375	A deep mechanistic characterization is extremely complex, but we can hypothesize that integrated changes in membrane structure and intracellular actin content could contribute to explain SRGF impact on ASC NF efficiency.
34943920	9	53	theme	mechanistic	1348:1358	arg1	complex					1390:1396	complex	1390:1396	complex	1390:1396	A deep mechanistic characterization is extremely complex, but we can hypothesize that integrated changes in membrane structure and intracellular actin content could contribute to explain SRGF impact on ASC NF efficiency.
34943920	0	54	theme	Stem/Stromal	37:48	arg1	Cells					50:54	Adipose Mesenchymal Stem/Stromal Cells	17:54	Adipose Mesenchymal Stem/Stromal Cells	17:54	Nucleofection of Adipose Mesenchymal Stem/Stromal Cells: Improved Transfection Efficiency for GMP Grade Applications.
34943920	6	55	theme	actin	856:860	arg1	content					862:868	intracellular actin content	842:868	intracellular actin content in ASC	842:875	SRGF increased n-6/n-3 ratio, reduced membrane lipid raft occurrence, and lowered intracellular actin content in ASC.
34943920	3	56	from	factors	442:448	arg1	rich					427:430	rich	427:430	rich	427:430	Supernatant rich in growth factors (SRGF) is a clinical-grade medium additive for ASC expansion.
34943920	1	57	theme	Practice	214:221	arg1	guidelines					223:232	Good Manufacturing Practice guidelines	195:232	Good Manufacturing Practice guidelines	195:232	Nucleofection (NF) is a safe, non-viral transfection method, compatible with Good Manufacturing Practice guidelines.
34943920	0	58	theme	Improved	57:64	arg1	Efficiency					79:88	Improved Transfection Efficiency	57:88	Nucleofection of Adipose Mesenchymal Stem/Stromal Cells: Improved Transfection Efficiency for GMP Grade Applications.	0:116	Nucleofection of Adipose Mesenchymal Stem/Stromal Cells: Improved Transfection Efficiency for GMP Grade Applications.
34943920	8	59	theme	first	1180:1184	arg1	time					1186:1189	the first time that tuning clinical-grade compatible cell culture conditions can significantly improve ASC transfection efficiency by a non-viral and safe approach	1176:1338	the first time that tuning clinical-grade compatible cell culture conditions can significantly improve ASC transfection efficiency by a non-viral and safe approach	1176:1338	In conclusion, we reported for the first time that tuning clinical-grade compatible cell culture conditions can significantly improve ASC transfection efficiency by a non-viral and safe approach.
34943920	6	60	theme	intracellular	842:854	arg1	content					862:868	intracellular actin content	842:868	intracellular actin content in ASC	842:875	SRGF increased n-6/n-3 ratio, reduced membrane lipid raft occurrence, and lowered intracellular actin content in ASC.
34943920	2	61	dep	tissue	310:315	arg1	ASC					341:343	ASC	341:343	ASC	341:343	Such a technique is useful to improve therapeutic effectiveness of adipose tissue mesenchymal stem cells (ASC) in clinical settings, but improvement of NF efficiency is mandatory.
34943920	2	61	dep	tissue	310:315	arg1	cells					334:338	mesenchymal stem cells	317:338	adipose tissue mesenchymal stem cells (ASC)	302:344	Such a technique is useful to improve therapeutic effectiveness of adipose tissue mesenchymal stem cells (ASC) in clinical settings, but improvement of NF efficiency is mandatory.
34943920	5	62	theme	increased	695:703	arg1	endocytosis					713:723	increased vesicle endocytosis	695:723	increased vesicle endocytosis	695:723	SRGF expanded ASC were characterized by increased vesicle endocytosis but lower phagocytosis properties.
34943920	1	63	with	compatible	179:188	arg1	guidelines					223:232	Good Manufacturing Practice guidelines	195:232	Good Manufacturing Practice guidelines	195:232	Nucleofection (NF) is a safe, non-viral transfection method, compatible with Good Manufacturing Practice guidelines.
34943920	9	64	theme	intracellular	1472:1484	arg1	content					1492:1498	intracellular actin content	1472:1498	intracellular actin content	1472:1498	A deep mechanistic characterization is extremely complex, but we can hypothesize that integrated changes in membrane structure and intracellular actin content could contribute to explain SRGF impact on ASC NF efficiency.
34943920	8	65	theme	ASC	1279:1281	arg1	efficiency					1296:1305	ASC transfection efficiency	1279:1305	ASC transfection efficiency	1279:1305	In conclusion, we reported for the first time that tuning clinical-grade compatible cell culture conditions can significantly improve ASC transfection efficiency by a non-viral and safe approach.
34943920	7	66	theme	NF	912:913	arg1	efficiency					915:924	NF efficiency	912:924	NF efficiency	912:924	A statistical correlation between NF efficiency and lipid raft availability on cell membranes was shown, even though a direct relationship could not be demonstrated: attempts to selectively modulate lipid rafts levels were, in fact, limited by technical constraints.
34943920	5	67	theme	expanded	660:667	arg1	ASC					669:671	SRGF expanded ASC	655:671	SRGF expanded ASC	655:671	SRGF expanded ASC were characterized by increased vesicle endocytosis but lower phagocytosis properties.
34943920	7	68	theme	direct	997:1002	arg1	relationship					1004:1015	a direct relationship	995:1015	a direct relationship	995:1015	A statistical correlation between NF efficiency and lipid raft availability on cell membranes was shown, even though a direct relationship could not be demonstrated: attempts to selectively modulate lipid rafts levels were, in fact, limited by technical constraints.
34943920	6	69	from	content	862:868	arg1	ASC					873:875	ASC	873:875	ASC	873:875	SRGF increased n-6/n-3 ratio, reduced membrane lipid raft occurrence, and lowered intracellular actin content in ASC.
34943920	5	70	dep	endocytosis	713:723	arg1	properties					748:757	properties	748:757	properties	748:757	SRGF expanded ASC were characterized by increased vesicle endocytosis but lower phagocytosis properties.
34943920	7	71	dep	rafts	1083:1087	arg1	limited					1111:1117	limited	1111:1117	limited by technical constraints	1111:1142	A statistical correlation between NF efficiency and lipid raft availability on cell membranes was shown, even though a direct relationship could not be demonstrated: attempts to selectively modulate lipid rafts levels were, in fact, limited by technical constraints.
34943920	8	72	theme	non-viral	1312:1320	arg1	approach					1331:1338	a non-viral and safe approach	1310:1338	a non-viral and safe approach	1310:1338	In conclusion, we reported for the first time that tuning clinical-grade compatible cell culture conditions can significantly improve ASC transfection efficiency by a non-viral and safe approach.
34943920	7	73	from	availability	941:952	arg1	membranes					962:970	cell membranes	957:970	cell membranes	957:970	A statistical correlation between NF efficiency and lipid raft availability on cell membranes was shown, even though a direct relationship could not be demonstrated: attempts to selectively modulate lipid rafts levels were, in fact, limited by technical constraints.
34943920	8	74	theme	safe	1326:1329	arg1	approach					1331:1338	a non-viral and safe approach	1310:1338	a non-viral and safe approach	1310:1338	In conclusion, we reported for the first time that tuning clinical-grade compatible cell culture conditions can significantly improve ASC transfection efficiency by a non-viral and safe approach.
34943920	2	75	theme	efficiency	390:399	arg1	improvement					372:382	improvement	372:382	improvement of NF efficiency	372:399	Such a technique is useful to improve therapeutic effectiveness of adipose tissue mesenchymal stem cells (ASC) in clinical settings, but improvement of NF efficiency is mandatory.
34943920	3	76	from	rich	427:430	arg1	SRGF					451:454	SRGF	451:454	SRGF	451:454	Supernatant rich in growth factors (SRGF) is a clinical-grade medium additive for ASC expansion.
34943920	3	76	from	rich	427:430	arg1	factors					442:448	growth factors	435:448	growth factors (SRGF)	435:455	Supernatant rich in growth factors (SRGF) is a clinical-grade medium additive for ASC expansion.
34943920	3	77	theme	ASC	497:499	arg1	expansion					501:509	ASC expansion	497:509	ASC expansion	497:509	Supernatant rich in growth factors (SRGF) is a clinical-grade medium additive for ASC expansion.
34943920	2	78	from	effectiveness	285:297	arg1	settings					358:365	clinical settings	349:365	clinical settings	349:365	Such a technique is useful to improve therapeutic effectiveness of adipose tissue mesenchymal stem cells (ASC) in clinical settings, but improvement of NF efficiency is mandatory.
34943920	0	79	theme	Transfection	66:77	arg1	Efficiency					79:88	Improved Transfection Efficiency	57:88	Nucleofection of Adipose Mesenchymal Stem/Stromal Cells: Improved Transfection Efficiency for GMP Grade Applications.	0:116	Nucleofection of Adipose Mesenchymal Stem/Stromal Cells: Improved Transfection Efficiency for GMP Grade Applications.
34943920	0	80	dep	Nucleofection	0:12	arg1	Efficiency					79:88	Improved Transfection Efficiency	57:88	Nucleofection of Adipose Mesenchymal Stem/Stromal Cells: Improved Transfection Efficiency for GMP Grade Applications.	0:116	Nucleofection of Adipose Mesenchymal Stem/Stromal Cells: Improved Transfection Efficiency for GMP Grade Applications.
34943920	9	81	from	changes	1438:1444	arg1	structure					1458:1466	membrane structure	1449:1466	membrane structure	1449:1466	A deep mechanistic characterization is extremely complex, but we can hypothesize that integrated changes in membrane structure and intracellular actin content could contribute to explain SRGF impact on ASC NF efficiency.
34943920	9	81	from	changes	1438:1444	arg1	content					1492:1498	intracellular actin content	1472:1498	intracellular actin content	1472:1498	A deep mechanistic characterization is extremely complex, but we can hypothesize that integrated changes in membrane structure and intracellular actin content could contribute to explain SRGF impact on ASC NF efficiency.
34943920	1	82	theme	safe	142:145	arg1	method					171:176	a safe, non-viral transfection method	140:176	a safe, non-viral transfection method	140:176	Nucleofection (NF) is a safe, non-viral transfection method, compatible with Good Manufacturing Practice guidelines.
34943920	1	82	theme	safe	142:145	arg1	Nucleofection					118:130	Nucleofection	118:130	Nucleofection (NF)	118:135	Nucleofection (NF) is a safe, non-viral transfection method, compatible with Good Manufacturing Practice guidelines.
32494972	6	0	theme	antibiotics	789:799	arg1	effects					778:784	the effects	774:784	the effects of antibiotics and prebiotics on WBRT-induced CD	774:833	In addition, we observed the effects of antibiotics and prebiotics on WBRT-induced CD.
32494972	12	1	theme	gut	1758:1760	arg1	microbiota					1762:1771	gut microbiota	1758:1771	gut microbiota	1758:1771	CONCLUSIONS WBRT-induced CD might be highly related to abnormal composition of gut microbiota.
32494972	3	2	theme	side	428:431	arg1	dysfunction					462:472	cognitive dysfunction	452:472	cognitive dysfunction (CD)	452:477	However, it cannot be ignored that WBRT might induce a series of neuropsychiatric side effects, including cognitive dysfunction (CD).
32494972	3	2	theme	side	428:431	arg1	effects					433:439	neuropsychiatric side effects	411:439	neuropsychiatric side effects	411:439	However, it cannot be ignored that WBRT might induce a series of neuropsychiatric side effects, including cognitive dysfunction (CD).
32494972	8	3	dep	RESULTS	919:925	arg1	revealed					960:967	revealed	960:967	revealed an altered composition of gut microbiota between CD and non-CD phenotypes	960:1041	RESULTS The 16S rRNA sequencing analysis revealed an altered composition of gut microbiota between CD and non-CD phenotypes.
32494972	4	4	theme	vital	577:581	arg1	role					583:586	a vital role	575:586	a vital role	575:586	Accumulating evidence shows that the gut microbiota and the gut-microbiota-brain axis may play a vital role in the pathogenesis of CD.
32494972	10	5	theme	01	1323:1324	arg1	level					1303:1307	the level	1299:1307	the level of Phylum-TM7 01	1299:1324	Pretreatment with antibiotics caused a significant decrease in the level of Phylum-TM7 01, whereas an increase in the levels of Class-Gammaproteobacteria, Order-Enterobacteriales, and Species-Escherichia coli.
32494972	3	6	theme	cognitive	452:460	arg1	dysfunction					462:472	cognitive dysfunction	452:472	cognitive dysfunction (CD)	452:477	However, it cannot be ignored that WBRT might induce a series of neuropsychiatric side effects, including cognitive dysfunction (CD).
32494972	3	6	theme	cognitive	452:460	arg1	CD					475:476	CD	475:476	CD	475:476	However, it cannot be ignored that WBRT might induce a series of neuropsychiatric side effects, including cognitive dysfunction (CD).
32494972	1	7	theme	brain	163:167	arg1	metastasis					169:178	brain metastasis	163:178	brain metastasis (BM)	163:183	RATIONALE Approximately 20-40% of patients with cancer will experience brain metastasis (BM), which has a great impact on the quality of life and survival rates of patients.
32494972	1	7	theme	brain	163:167	arg1	BM					181:182	BM	181:182	BM	181:182	RATIONALE Approximately 20-40% of patients with cancer will experience brain metastasis (BM), which has a great impact on the quality of life and survival rates of patients.
32494972	10	8	with	Pretreatment	1236:1247	arg1	antibiotics					1254:1264	antibiotics	1254:1264	antibiotics	1254:1264	Pretreatment with antibiotics caused a significant decrease in the level of Phylum-TM7 01, whereas an increase in the levels of Class-Gammaproteobacteria, Order-Enterobacteriales, and Species-Escherichia coli.
32494972	6	9	theme	WBRT-induced	819:830	arg1	CD					832:833	WBRT-induced CD	819:833	WBRT-induced CD	819:833	In addition, we observed the effects of antibiotics and prebiotics on WBRT-induced CD.
32494972	1	10	from	impact	204:209	arg1	rates					247:251	survival rates	238:251	survival rates of patients	238:263	RATIONALE Approximately 20-40% of patients with cancer will experience brain metastasis (BM), which has a great impact on the quality of life and survival rates of patients.
32494972	1	10	from	impact	204:209	arg1	quality					218:224	the quality	214:224	the quality of life	214:232	RATIONALE Approximately 20-40% of patients with cancer will experience brain metastasis (BM), which has a great impact on the quality of life and survival rates of patients.
32494972	9	11	theme	Order-Bacteroidales	1141:1159	arg1	levels					1087:1092	the levels	1083:1092	the levels of Phylum-Bacteroidete, Class-Bacteroidia, and Order-Bacteroidales in the CD group	1083:1175	Furthermore, we observed a decrease in the levels of Phylum-Bacteroidete, Class-Bacteroidia, and Order-Bacteroidales in the CD group and an increase in the Genus-Allobaculum level after WBRT.
32494972	1	12	contain	has	192:194	arg1	metastasis					169:178	brain metastasis	163:178	brain metastasis (BM)	163:183	RATIONALE Approximately 20-40% of patients with cancer will experience brain metastasis (BM), which has a great impact on the quality of life and survival rates of patients.
32494972	1	12	contain	has	192:194	arg2	impact					204:209	a great impact	196:209	a great impact on the quality of life and survival rates of patients	196:263	RATIONALE Approximately 20-40% of patients with cancer will experience brain metastasis (BM), which has a great impact on the quality of life and survival rates of patients.
32494972	1	12	contain	has	192:194	arg1	BM					181:182	BM	181:182	BM	181:182	RATIONALE Approximately 20-40% of patients with cancer will experience brain metastasis (BM), which has a great impact on the quality of life and survival rates of patients.
32494972	7	13	theme	sequencing	878:887	arg1	analysis					889:896	the 16S rRNA sequencing analysis	865:896	the 16S rRNA sequencing analysis	865:896	Variations were revealed via the 16S rRNA sequencing analysis at different levels.
32494972	6	14	theme	prebiotics	805:814	arg1	effects					778:784	the effects	774:784	the effects of antibiotics and prebiotics on WBRT-induced CD	774:833	In addition, we observed the effects of antibiotics and prebiotics on WBRT-induced CD.
32494972	7	15	theme	16S	869:871	arg1	analysis					889:896	the 16S rRNA sequencing analysis	865:896	the 16S rRNA sequencing analysis	865:896	Variations were revealed via the 16S rRNA sequencing analysis at different levels.
32494972	9	16	from	decrease	1071:1078	arg1	levels					1087:1092	the levels	1083:1092	the levels of Phylum-Bacteroidete, Class-Bacteroidia, and Order-Bacteroidales in the CD group	1083:1175	Furthermore, we observed a decrease in the levels of Phylum-Bacteroidete, Class-Bacteroidia, and Order-Bacteroidales in the CD group and an increase in the Genus-Allobaculum level after WBRT.
32494972	9	16	from	decrease	1071:1078	arg1	level					1218:1222	the Genus-Allobaculum level	1196:1222	the Genus-Allobaculum level after WBRT	1196:1233	Furthermore, we observed a decrease in the levels of Phylum-Bacteroidete, Class-Bacteroidia, and Order-Bacteroidales in the CD group and an increase in the Genus-Allobaculum level after WBRT.
32494972	4	17	theme	CD	611:612	arg1	pathogenesis					595:606	the pathogenesis	591:606	the pathogenesis of CD	591:612	Accumulating evidence shows that the gut microbiota and the gut-microbiota-brain axis may play a vital role in the pathogenesis of CD.
32494972	0	18	theme	gut	76:78	arg1	microbiota					80:89	gut microbiota	76:89	gut microbiota	76:89	Whole brain radiotherapy induces cognitive dysfunction in mice: key role of gut microbiota.
32494972	9	19	theme	Genus-Allobaculum	1200:1216	arg1	level					1218:1222	the Genus-Allobaculum level	1196:1222	the Genus-Allobaculum level after WBRT	1196:1233	Furthermore, we observed a decrease in the levels of Phylum-Bacteroidete, Class-Bacteroidia, and Order-Bacteroidales in the CD group and an increase in the Genus-Allobaculum level after WBRT.
32494972	8	20	theme	16S	931:933	arg1	analysis					951:958	The 16S rRNA sequencing analysis	927:958	The 16S rRNA sequencing analysis	927:958	RESULTS The 16S rRNA sequencing analysis revealed an altered composition of gut microbiota between CD and non-CD phenotypes.
32494972	0	21	theme	key	64:66	arg1	role					68:71	key role	64:71	key role of gut microbiota	64:89	Whole brain radiotherapy induces cognitive dysfunction in mice: key role of gut microbiota.
32494972	13	22	theme	beneficial	1845:1854	arg1	effects					1856:1862	beneficial effects	1845:1862	beneficial effects on CD in individuals exposed to WBRT	1845:1899	Strategies improving the composition of the gut microbiota may provide beneficial effects on CD in individuals exposed to WBRT.
32494972	0	23	theme	Whole	0:4	arg1	radiotherapy					12:23	Whole brain radiotherapy	0:23	Whole brain radiotherapy	0:23	Whole brain radiotherapy induces cognitive dysfunction in mice: key role of gut microbiota.
32494972	1	24	theme	life	229:232	arg1	rates					247:251	survival rates	238:251	survival rates of patients	238:263	RATIONALE Approximately 20-40% of patients with cancer will experience brain metastasis (BM), which has a great impact on the quality of life and survival rates of patients.
32494972	1	24	theme	life	229:232	arg1	quality					218:224	the quality	214:224	the quality of life	214:232	RATIONALE Approximately 20-40% of patients with cancer will experience brain metastasis (BM), which has a great impact on the quality of life and survival rates of patients.
32494972	8	25	theme	sequencing	940:949	arg1	analysis					951:958	The 16S rRNA sequencing analysis	927:958	The 16S rRNA sequencing analysis	927:958	RESULTS The 16S rRNA sequencing analysis revealed an altered composition of gut microbiota between CD and non-CD phenotypes.
32494972	5	26	theme	maze	723:726	arg1	results					740:746	the Morris water maze test (MWMT) results	706:746	the Morris water maze test (MWMT) results	706:746	OBJECTIVE AND METHODS We adopted WBRT to mimic CD after a hierarchical cluster analysis of the Morris water maze test (MWMT) results.
32494972	1	27	theme	survival	238:245	arg1	rates					247:251	survival rates	238:251	survival rates of patients	238:263	RATIONALE Approximately 20-40% of patients with cancer will experience brain metastasis (BM), which has a great impact on the quality of life and survival rates of patients.
32494972	11	28	theme	distasonis	1652:1661	arg1	levels					1572:1577	the levels	1568:1577	the levels of Family-Bacteroidaceae, Genus-Bacteroides, and Species-Parabacteroides distasonis	1568:1661	After pretreatment with probiotics, the levels of Phylum-Cyanobacteria, Class-4C0d-2, and Order-YS2 were decreased, while the levels of Family-Bacteroidaceae, Genus-Bacteroides, and Species-Parabacteroides distasonis were increased.
32494972	13	29	theme	gut	1818:1820	arg1	microbiota					1822:1831	the gut microbiota	1814:1831	the gut microbiota	1814:1831	Strategies improving the composition of the gut microbiota may provide beneficial effects on CD in individuals exposed to WBRT.
32494972	2	30	theme	BM	342:343	arg1	treatment					329:337	the treatment	325:337	the treatment of BM	325:343	Whole brain radiotherapy (WBRT) is an effective method for the treatment of BM.
32494972	0	31	theme	cognitive	33:41	arg1	dysfunction					43:53	cognitive dysfunction	33:53	cognitive dysfunction	33:53	Whole brain radiotherapy induces cognitive dysfunction in mice: key role of gut microbiota.
32494972	5	32	theme	cluster	686:692	arg1	analysis					694:701	a hierarchical cluster analysis	671:701	a hierarchical cluster analysis of the Morris water maze test (MWMT) results	671:746	OBJECTIVE AND METHODS We adopted WBRT to mimic CD after a hierarchical cluster analysis of the Morris water maze test (MWMT) results.
32494972	11	33	with	pretreatment	1452:1463	arg1	probiotics					1470:1479	probiotics	1470:1479	probiotics	1470:1479	After pretreatment with probiotics, the levels of Phylum-Cyanobacteria, Class-4C0d-2, and Order-YS2 were decreased, while the levels of Family-Bacteroidaceae, Genus-Bacteroides, and Species-Parabacteroides distasonis were increased.
32494972	8	34	theme	altered	972:978	arg1	composition					980:990	an altered composition	969:990	an altered composition of gut microbiota between CD and non-CD phenotypes	969:1041	RESULTS The 16S rRNA sequencing analysis revealed an altered composition of gut microbiota between CD and non-CD phenotypes.
32494972	10	35	theme	Species-Escherichia	1420:1438	arg1	levels					1354:1359	the levels	1350:1359	the levels of Class-Gammaproteobacteria, Order-Enterobacteriales, and Species-Escherichia coli	1350:1443	Pretreatment with antibiotics caused a significant decrease in the level of Phylum-TM7 01, whereas an increase in the levels of Class-Gammaproteobacteria, Order-Enterobacteriales, and Species-Escherichia coli.
32494972	8	36	theme	microbiota	999:1008	arg1	composition					980:990	an altered composition	969:990	an altered composition of gut microbiota between CD and non-CD phenotypes	969:1041	RESULTS The 16S rRNA sequencing analysis revealed an altered composition of gut microbiota between CD and non-CD phenotypes.
32494972	4	37	theme	gut	517:519	arg1	microbiota					521:530	the gut microbiota	513:530	the gut microbiota	513:530	Accumulating evidence shows that the gut microbiota and the gut-microbiota-brain axis may play a vital role in the pathogenesis of CD.
32494972	3	38	theme	effects	433:439	arg1	series					401:406	a series	399:406	a series of neuropsychiatric side effects, including cognitive dysfunction (CD)	399:477	However, it cannot be ignored that WBRT might induce a series of neuropsychiatric side effects, including cognitive dysfunction (CD).
32494972	1	39	theme	great	198:202	arg1	impact					204:209	a great impact	196:209	a great impact on the quality of life and survival rates of patients	196:263	RATIONALE Approximately 20-40% of patients with cancer will experience brain metastasis (BM), which has a great impact on the quality of life and survival rates of patients.
32494972	8	40	theme	CD	1018:1019	arg1	phenotypes					1032:1041	CD and non-CD phenotypes	1018:1041	CD and non-CD phenotypes	1018:1041	RESULTS The 16S rRNA sequencing analysis revealed an altered composition of gut microbiota between CD and non-CD phenotypes.
32494972	12	41	theme	microbiota	1762:1771	arg1	composition					1743:1753	abnormal composition	1734:1753	abnormal composition of gut microbiota	1734:1771	CONCLUSIONS WBRT-induced CD might be highly related to abnormal composition of gut microbiota.
32494972	11	42	theme	Order-YS2	1536:1544	arg1	levels					1486:1491	the levels	1482:1491	the levels of Phylum-Cyanobacteria, Class-4C0d-2, and Order-YS2	1482:1544	After pretreatment with probiotics, the levels of Phylum-Cyanobacteria, Class-4C0d-2, and Order-YS2 were decreased, while the levels of Family-Bacteroidaceae, Genus-Bacteroides, and Species-Parabacteroides distasonis were increased.
32494972	3	43	theme	neuropsychiatric	411:426	arg1	dysfunction					462:472	cognitive dysfunction	452:472	cognitive dysfunction (CD)	452:477	However, it cannot be ignored that WBRT might induce a series of neuropsychiatric side effects, including cognitive dysfunction (CD).
32494972	3	43	theme	neuropsychiatric	411:426	arg1	effects					433:439	neuropsychiatric side effects	411:439	neuropsychiatric side effects	411:439	However, it cannot be ignored that WBRT might induce a series of neuropsychiatric side effects, including cognitive dysfunction (CD).
32494972	8	44	theme	non-CD	1025:1030	arg1	phenotypes					1032:1041	CD and non-CD phenotypes	1018:1041	CD and non-CD phenotypes	1018:1041	RESULTS The 16S rRNA sequencing analysis revealed an altered composition of gut microbiota between CD and non-CD phenotypes.
32494972	12	45	theme	abnormal	1734:1741	arg1	composition					1743:1753	abnormal composition	1734:1753	abnormal composition of gut microbiota	1734:1771	CONCLUSIONS WBRT-induced CD might be highly related to abnormal composition of gut microbiota.
32494972	11	46	theme	Genus-Bacteroides	1605:1621	arg1	levels					1572:1577	the levels	1568:1577	the levels of Family-Bacteroidaceae, Genus-Bacteroides, and Species-Parabacteroides distasonis	1568:1661	After pretreatment with probiotics, the levels of Phylum-Cyanobacteria, Class-4C0d-2, and Order-YS2 were decreased, while the levels of Family-Bacteroidaceae, Genus-Bacteroides, and Species-Parabacteroides distasonis were increased.
32494972	10	47	theme	significant	1275:1285	arg1	decrease					1287:1294	a significant decrease	1273:1294	a significant decrease in the level of Phylum-TM7 01	1273:1324	Pretreatment with antibiotics caused a significant decrease in the level of Phylum-TM7 01, whereas an increase in the levels of Class-Gammaproteobacteria, Order-Enterobacteriales, and Species-Escherichia coli.
32494972	5	48	theme	results	740:746	arg1	analysis					694:701	a hierarchical cluster analysis	671:701	a hierarchical cluster analysis of the Morris water maze test (MWMT) results	671:746	OBJECTIVE AND METHODS We adopted WBRT to mimic CD after a hierarchical cluster analysis of the Morris water maze test (MWMT) results.
32494972	12	49	theme	CD	1704:1705	arg1	CONCLUSIONS					1679:1689	CONCLUSIONS	1679:1689	CONCLUSIONS WBRT-induced CD	1679:1705	CONCLUSIONS WBRT-induced CD might be highly related to abnormal composition of gut microbiota.
32494972	11	50	theme	Family-Bacteroidaceae	1582:1602	arg1	levels					1572:1577	the levels	1568:1577	the levels of Family-Bacteroidaceae, Genus-Bacteroides, and Species-Parabacteroides distasonis	1568:1661	After pretreatment with probiotics, the levels of Phylum-Cyanobacteria, Class-4C0d-2, and Order-YS2 were decreased, while the levels of Family-Bacteroidaceae, Genus-Bacteroides, and Species-Parabacteroides distasonis were increased.
32494972	10	51	theme	Phylum-TM7	1312:1321	arg1	01					1323:1324	Phylum-TM7 01	1312:1324	Phylum-TM7 01	1312:1324	Pretreatment with antibiotics caused a significant decrease in the level of Phylum-TM7 01, whereas an increase in the levels of Class-Gammaproteobacteria, Order-Enterobacteriales, and Species-Escherichia coli.
32494972	2	52	theme	Whole	266:270	arg1	method					314:319	an effective method	301:319	an effective method for the treatment of BM	301:343	Whole brain radiotherapy (WBRT) is an effective method for the treatment of BM.
32494972	2	52	theme	Whole	266:270	arg1	WBRT					292:295	WBRT	292:295	WBRT	292:295	Whole brain radiotherapy (WBRT) is an effective method for the treatment of BM.
32494972	2	52	theme	Whole	266:270	arg1	radiotherapy					278:289	Whole brain radiotherapy	266:289	Whole brain radiotherapy (WBRT)	266:296	Whole brain radiotherapy (WBRT) is an effective method for the treatment of BM.
32494972	9	53	theme	Phylum-Bacteroidete	1097:1115	arg1	levels					1087:1092	the levels	1083:1092	the levels of Phylum-Bacteroidete, Class-Bacteroidia, and Order-Bacteroidales in the CD group	1083:1175	Furthermore, we observed a decrease in the levels of Phylum-Bacteroidete, Class-Bacteroidia, and Order-Bacteroidales in the CD group and an increase in the Genus-Allobaculum level after WBRT.
32494972	5	54	theme	test	728:731	arg1	results					740:746	the Morris water maze test (MWMT) results	706:746	the Morris water maze test (MWMT) results	706:746	OBJECTIVE AND METHODS We adopted WBRT to mimic CD after a hierarchical cluster analysis of the Morris water maze test (MWMT) results.
32494972	10	55	from	increase	1338:1345	arg1	levels					1354:1359	the levels	1350:1359	the levels of Class-Gammaproteobacteria, Order-Enterobacteriales, and Species-Escherichia coli	1350:1443	Pretreatment with antibiotics caused a significant decrease in the level of Phylum-TM7 01, whereas an increase in the levels of Class-Gammaproteobacteria, Order-Enterobacteriales, and Species-Escherichia coli.
32494972	4	56	theme	gut-microbiota-brain	540:559	arg1	axis					561:564	the gut-microbiota-brain axis	536:564	the gut-microbiota-brain axis	536:564	Accumulating evidence shows that the gut microbiota and the gut-microbiota-brain axis may play a vital role in the pathogenesis of CD.
32494972	9	57	theme	Class-Bacteroidia	1118:1134	arg1	levels					1087:1092	the levels	1083:1092	the levels of Phylum-Bacteroidete, Class-Bacteroidia, and Order-Bacteroidales in the CD group	1083:1175	Furthermore, we observed a decrease in the levels of Phylum-Bacteroidete, Class-Bacteroidia, and Order-Bacteroidales in the CD group and an increase in the Genus-Allobaculum level after WBRT.
32494972	5	58	theme	MWMT	734:737	arg1	results					740:746	the Morris water maze test (MWMT) results	706:746	the Morris water maze test (MWMT) results	706:746	OBJECTIVE AND METHODS We adopted WBRT to mimic CD after a hierarchical cluster analysis of the Morris water maze test (MWMT) results.
32494972	9	59	from	increase	1184:1191	arg1	levels					1087:1092	the levels	1083:1092	the levels of Phylum-Bacteroidete, Class-Bacteroidia, and Order-Bacteroidales in the CD group	1083:1175	Furthermore, we observed a decrease in the levels of Phylum-Bacteroidete, Class-Bacteroidia, and Order-Bacteroidales in the CD group and an increase in the Genus-Allobaculum level after WBRT.
32494972	9	59	from	increase	1184:1191	arg1	level					1218:1222	the Genus-Allobaculum level	1196:1222	the Genus-Allobaculum level after WBRT	1196:1233	Furthermore, we observed a decrease in the levels of Phylum-Bacteroidete, Class-Bacteroidia, and Order-Bacteroidales in the CD group and an increase in the Genus-Allobaculum level after WBRT.
32494972	7	60	theme	different	901:909	arg1	levels					911:916	different levels	901:916	different levels	901:916	Variations were revealed via the 16S rRNA sequencing analysis at different levels.
32494972	9	61	from	levels	1087:1092	arg1	group					1171:1175	the CD group	1164:1175	the CD group	1164:1175	Furthermore, we observed a decrease in the levels of Phylum-Bacteroidete, Class-Bacteroidia, and Order-Bacteroidales in the CD group and an increase in the Genus-Allobaculum level after WBRT.
32494972	12	62	dep	CD	1704:1705	arg1	WBRT-induced					1691:1702	WBRT-induced	1691:1702	WBRT-induced	1691:1702	CONCLUSIONS WBRT-induced CD might be highly related to abnormal composition of gut microbiota.
32494972	10	63	dep	Species-Escherichia	1420:1438	arg1	coli					1440:1443	Species-Escherichia coli	1420:1443	Species-Escherichia coli	1420:1443	Pretreatment with antibiotics caused a significant decrease in the level of Phylum-TM7 01, whereas an increase in the levels of Class-Gammaproteobacteria, Order-Enterobacteriales, and Species-Escherichia coli.
32494972	9	64	theme	CD	1168:1169	arg1	group					1171:1175	the CD group	1164:1175	the CD group	1164:1175	Furthermore, we observed a decrease in the levels of Phylum-Bacteroidete, Class-Bacteroidia, and Order-Bacteroidales in the CD group and an increase in the Genus-Allobaculum level after WBRT.
32494972	7	65	theme	rRNA	873:876	arg1	analysis					889:896	the 16S rRNA sequencing analysis	865:896	the 16S rRNA sequencing analysis	865:896	Variations were revealed via the 16S rRNA sequencing analysis at different levels.
32494972	1	66	theme	patients	126:133	arg1	RATIONALE					92:100	RATIONALE	92:100	RATIONALE Approximately 20-40% of patients with cancer	92:145	RATIONALE Approximately 20-40% of patients with cancer will experience brain metastasis (BM), which has a great impact on the quality of life and survival rates of patients.
32494972	1	67	dep	RATIONALE	92:100	arg1	%					121:121	Approximately 20-40%	102:121	RATIONALE Approximately 20-40% of patients with cancer	92:145	RATIONALE Approximately 20-40% of patients with cancer will experience brain metastasis (BM), which has a great impact on the quality of life and survival rates of patients.
32494972	5	68	dep	OBJECTIVE	615:623	arg1	adopted					640:646	adopted	640:646	adopted WBRT to mimic CD after a hierarchical cluster analysis of the Morris water maze test (MWMT) results	640:746	OBJECTIVE AND METHODS We adopted WBRT to mimic CD after a hierarchical cluster analysis of the Morris water maze test (MWMT) results.
32494972	0	69	theme	microbiota	80:89	arg1	role					68:71	key role	64:71	key role of gut microbiota	64:89	Whole brain radiotherapy induces cognitive dysfunction in mice: key role of gut microbiota.
32494972	1	70	with	patients	126:133	arg1	cancer					140:145	cancer	140:145	cancer	140:145	RATIONALE Approximately 20-40% of patients with cancer will experience brain metastasis (BM), which has a great impact on the quality of life and survival rates of patients.
32494972	13	71	theme	microbiota	1822:1831	arg1	composition					1799:1809	the composition	1795:1809	the composition of the gut microbiota	1795:1831	Strategies improving the composition of the gut microbiota may provide beneficial effects on CD in individuals exposed to WBRT.
32494972	8	72	theme	rRNA	935:938	arg1	analysis					951:958	The 16S rRNA sequencing analysis	927:958	The 16S rRNA sequencing analysis	927:958	RESULTS The 16S rRNA sequencing analysis revealed an altered composition of gut microbiota between CD and non-CD phenotypes.
32494972	13	73	from	effects	1856:1862	arg1	CD					1867:1868	CD	1867:1868	CD	1867:1868	Strategies improving the composition of the gut microbiota may provide beneficial effects on CD in individuals exposed to WBRT.
32494972	13	73	from	effects	1856:1862	arg1	individuals					1873:1883	individuals	1873:1883	individuals exposed to WBRT	1873:1899	Strategies improving the composition of the gut microbiota may provide beneficial effects on CD in individuals exposed to WBRT.
32494972	0	74	theme	brain	6:10	arg1	radiotherapy					12:23	Whole brain radiotherapy	0:23	Whole brain radiotherapy	0:23	Whole brain radiotherapy induces cognitive dysfunction in mice: key role of gut microbiota.
32494972	5	75	theme	water	717:721	arg1	maze					723:726	Morris water maze	710:726	the Morris water maze test (MWMT) results	706:746	OBJECTIVE AND METHODS We adopted WBRT to mimic CD after a hierarchical cluster analysis of the Morris water maze test (MWMT) results.
32494972	5	76	theme	Morris	710:715	arg1	maze					723:726	Morris water maze	710:726	the Morris water maze test (MWMT) results	706:746	OBJECTIVE AND METHODS We adopted WBRT to mimic CD after a hierarchical cluster analysis of the Morris water maze test (MWMT) results.
32494972	4	77	theme	Accumulating	480:491	arg1	evidence					493:500	Accumulating evidence	480:500	Accumulating evidence	480:500	Accumulating evidence shows that the gut microbiota and the gut-microbiota-brain axis may play a vital role in the pathogenesis of CD.
32494972	10	78	theme	Order-Enterobacteriales	1391:1413	arg1	levels					1354:1359	the levels	1350:1359	the levels of Class-Gammaproteobacteria, Order-Enterobacteriales, and Species-Escherichia coli	1350:1443	Pretreatment with antibiotics caused a significant decrease in the level of Phylum-TM7 01, whereas an increase in the levels of Class-Gammaproteobacteria, Order-Enterobacteriales, and Species-Escherichia coli.
32494972	11	79	theme	Species-Parabacteroides	1628:1650	arg1	distasonis					1652:1661	Species-Parabacteroides distasonis	1628:1661	Species-Parabacteroides distasonis	1628:1661	After pretreatment with probiotics, the levels of Phylum-Cyanobacteria, Class-4C0d-2, and Order-YS2 were decreased, while the levels of Family-Bacteroidaceae, Genus-Bacteroides, and Species-Parabacteroides distasonis were increased.
32494972	2	80	theme	effective	304:312	arg1	method					314:319	an effective method	301:319	an effective method for the treatment of BM	301:343	Whole brain radiotherapy (WBRT) is an effective method for the treatment of BM.
32494972	2	80	theme	effective	304:312	arg1	radiotherapy					278:289	Whole brain radiotherapy	266:289	Whole brain radiotherapy (WBRT)	266:296	Whole brain radiotherapy (WBRT) is an effective method for the treatment of BM.
32494972	5	81	theme	hierarchical	673:684	arg1	analysis					694:701	a hierarchical cluster analysis	671:701	a hierarchical cluster analysis of the Morris water maze test (MWMT) results	671:746	OBJECTIVE AND METHODS We adopted WBRT to mimic CD after a hierarchical cluster analysis of the Morris water maze test (MWMT) results.
32494972	10	82	theme	Class-Gammaproteobacteria	1364:1388	arg1	levels					1354:1359	the levels	1350:1359	the levels of Class-Gammaproteobacteria, Order-Enterobacteriales, and Species-Escherichia coli	1350:1443	Pretreatment with antibiotics caused a significant decrease in the level of Phylum-TM7 01, whereas an increase in the levels of Class-Gammaproteobacteria, Order-Enterobacteriales, and Species-Escherichia coli.
32494972	11	83	theme	Class-4C0d-2	1518:1529	arg1	levels					1486:1491	the levels	1482:1491	the levels of Phylum-Cyanobacteria, Class-4C0d-2, and Order-YS2	1482:1544	After pretreatment with probiotics, the levels of Phylum-Cyanobacteria, Class-4C0d-2, and Order-YS2 were decreased, while the levels of Family-Bacteroidaceae, Genus-Bacteroides, and Species-Parabacteroides distasonis were increased.
32494972	1	84	theme	patients	256:263	arg1	rates					247:251	survival rates	238:251	survival rates of patients	238:263	RATIONALE Approximately 20-40% of patients with cancer will experience brain metastasis (BM), which has a great impact on the quality of life and survival rates of patients.
32494972	1	84	theme	patients	256:263	arg1	quality					218:224	the quality	214:224	the quality of life	214:232	RATIONALE Approximately 20-40% of patients with cancer will experience brain metastasis (BM), which has a great impact on the quality of life and survival rates of patients.
32494972	8	85	theme	gut	995:997	arg1	microbiota					999:1008	gut microbiota	995:1008	gut microbiota	995:1008	RESULTS The 16S rRNA sequencing analysis revealed an altered composition of gut microbiota between CD and non-CD phenotypes.
32494972	10	86	from	decrease	1287:1294	arg1	level					1303:1307	the level	1299:1307	the level of Phylum-TM7 01	1299:1324	Pretreatment with antibiotics caused a significant decrease in the level of Phylum-TM7 01, whereas an increase in the levels of Class-Gammaproteobacteria, Order-Enterobacteriales, and Species-Escherichia coli.
32494972	11	87	theme	Phylum-Cyanobacteria	1496:1515	arg1	levels					1486:1491	the levels	1482:1491	the levels of Phylum-Cyanobacteria, Class-4C0d-2, and Order-YS2	1482:1544	After pretreatment with probiotics, the levels of Phylum-Cyanobacteria, Class-4C0d-2, and Order-YS2 were decreased, while the levels of Family-Bacteroidaceae, Genus-Bacteroides, and Species-Parabacteroides distasonis were increased.
32494972	2	88	theme	brain	272:276	arg1	method					314:319	an effective method	301:319	an effective method for the treatment of BM	301:343	Whole brain radiotherapy (WBRT) is an effective method for the treatment of BM.
32494972	2	88	theme	brain	272:276	arg1	WBRT					292:295	WBRT	292:295	WBRT	292:295	Whole brain radiotherapy (WBRT) is an effective method for the treatment of BM.
32494972	2	88	theme	brain	272:276	arg1	radiotherapy					278:289	Whole brain radiotherapy	266:289	Whole brain radiotherapy (WBRT)	266:296	Whole brain radiotherapy (WBRT) is an effective method for the treatment of BM.
32494972	6	89	from	effects	778:784	arg1	CD					832:833	WBRT-induced CD	819:833	WBRT-induced CD	819:833	In addition, we observed the effects of antibiotics and prebiotics on WBRT-induced CD.
33861772	2	0	theme	knee	414:417	arg1	SF					419:420	normal knee SF	407:420	normal knee SF	407:420	Our study aimed to quantify species-dependent differences in phospholipid (PL) profiles of normal knee SF obtained from equine and human donors.
33861772	0	1	from	study	14:18	arg1	lipidome					27:34	the lipidome	23:34	the lipidome of normal knee synovial fluid from humans and horses	23:87	A comparative study on the lipidome of normal knee synovial fluid from humans and horses.
33861772	1	2	theme	fluid	137:141	arg1	components					148:157	synovial fluid (SF) components	128:157	synovial fluid (SF) components in health and disease	128:179	The current limitations in evaluating synovial fluid (SF) components in health and disease and between species are due in part to the lack of data on normal SF, because of low availability of SF from healthy articular joints.
33861772	0	3	theme	fluid	60:64	arg1	lipidome					27:34	the lipidome	23:34	the lipidome of normal knee synovial fluid from humans and horses	23:87	A comparative study on the lipidome of normal knee synovial fluid from humans and horses.
33861772	3	4	theme	equine	554:559	arg1	donors					561:566	15 and 13 joint-healthy human and equine donors	520:566	15 and 13 joint-healthy human and equine donors	520:566	Knee SF was obtained during autopsy by arthrocentesis from 15 and 13 joint-healthy human and equine donors, respectively.
33861772	2	5	theme	human	447:451	arg1	donors					453:458	equine and human donors	436:458	donors	453:458	Our study aimed to quantify species-dependent differences in phospholipid (PL) profiles of normal knee SF obtained from equine and human donors.
33861772	3	6	theme	Knee	461:464	arg1	SF					466:467	Knee SF	461:467	Knee SF	461:467	Knee SF was obtained during autopsy by arthrocentesis from 15 and 13 joint-healthy human and equine donors, respectively.
33861772	1	7	theme	SF	282:283	arg1	availability					266:277	low availability	262:277	low availability of SF from healthy articular joints	262:313	The current limitations in evaluating synovial fluid (SF) components in health and disease and between species are due in part to the lack of data on normal SF, because of low availability of SF from healthy articular joints.
33861772	1	8	from	SF	247:248	arg1	lack					224:227	the lack	220:227	the lack of data on normal SF	220:248	The current limitations in evaluating synovial fluid (SF) components in health and disease and between species are due in part to the lack of data on normal SF, because of low availability of SF from healthy articular joints.
33861772	7	9	theme	PL	1136:1137	arg1	composition					1139:1149	PL composition	1136:1149	PL composition	1136:1149	Importantly, equine SF contains about half of the PL content determined in human SF with some characteristic changes in PL composition.
33861772	9	10	theme	articular	1435:1443	arg1	lubrication					1445:1455	articular lubrication	1435:1455	articular lubrication	1435:1455	Our study provides comprehensive quantitative data on PL species levels in normal human and equine knee SF so that research in joint diseases and articular lubrication can be facilitated.
33861772	1	11	from	joints	308:313	arg1	availability					266:277	low availability	262:277	low availability of SF from healthy articular joints	262:313	The current limitations in evaluating synovial fluid (SF) components in health and disease and between species are due in part to the lack of data on normal SF, because of low availability of SF from healthy articular joints.
33861772	1	11	from	joints	308:313	arg1	SF					282:283	SF	282:283	SF from healthy articular joints	282:313	The current limitations in evaluating synovial fluid (SF) components in health and disease and between species are due in part to the lack of data on normal SF, because of low availability of SF from healthy articular joints.
33861772	1	12	from	data	232:235	arg1	SF					247:248	normal SF	240:248	normal SF	240:248	The current limitations in evaluating synovial fluid (SF) components in health and disease and between species are due in part to the lack of data on normal SF, because of low availability of SF from healthy articular joints.
33861772	5	13	theme	sum	719:721	arg1	test					723:726	Wilcoxon's rank sum test	703:726	Wilcoxon's rank sum test with adjustment of scores for tied values	703:768	Wilcoxon's rank sum test with adjustment of scores for tied values was applied followed by Holm´s method to account for multiple testing.
33861772	8	14	theme	decreased	1172:1180	arg1	levels					1197:1202	decreased apolipoprotein levels	1172:1202	decreased apolipoprotein levels	1172:1202	Nutritional habits, decreased apolipoprotein levels and altered enzymatic activities may have caused the observed different PL profiles.
33861772	8	15	theme	different	1266:1274	arg1	profiles					1279:1286	the observed different PL profiles	1253:1286	the observed different PL profiles	1253:1286	Nutritional habits, decreased apolipoprotein levels and altered enzymatic activities may have caused the observed different PL profiles.
33861772	2	16	theme	normal	407:412	arg1	SF					419:420	normal knee SF	407:420	normal knee SF	407:420	Our study aimed to quantify species-dependent differences in phospholipid (PL) profiles of normal knee SF obtained from equine and human donors.
33861772	0	17	from	lipidome	27:34	arg1	horses					82:87	horses	82:87	horses	82:87	A comparative study on the lipidome of normal knee synovial fluid from humans and horses.
33861772	0	17	from	lipidome	27:34	arg1	humans					71:76	humans	71:76	humans	71:76	A comparative study on the lipidome of normal knee synovial fluid from humans and horses.
33861772	7	18	theme	PL	1066:1067	arg1	content					1069:1075	the PL content	1062:1075	the PL content determined in human SF with some characteristic changes in PL composition	1062:1149	Importantly, equine SF contains about half of the PL content determined in human SF with some characteristic changes in PL composition.
33861772	7	19	theme	characteristic	1110:1123	arg1	changes					1125:1131	some characteristic changes	1105:1131	some characteristic changes in PL composition	1105:1149	Importantly, equine SF contains about half of the PL content determined in human SF with some characteristic changes in PL composition.
33861772	2	20	theme	equine	436:441	arg1	donors					453:458	equine and human donors	436:458	donors	453:458	Our study aimed to quantify species-dependent differences in phospholipid (PL) profiles of normal knee SF obtained from equine and human donors.
33861772	0	21	theme	comparative	2:12	arg1	study					14:18	A comparative study	0:18	A comparative study on the lipidome of normal knee synovial fluid from humans and horses	0:87	A comparative study on the lipidome of normal knee synovial fluid from humans and horses.
33861772	1	22	theme	articular	298:306	arg1	joints					308:313	healthy articular joints	290:313	healthy articular joints	290:313	The current limitations in evaluating synovial fluid (SF) components in health and disease and between species are due in part to the lack of data on normal SF, because of low availability of SF from healthy articular joints.
33861772	1	23	from	lack	224:227	arg1	SF					247:248	normal SF	240:248	normal SF	240:248	The current limitations in evaluating synovial fluid (SF) components in health and disease and between species are due in part to the lack of data on normal SF, because of low availability of SF from healthy articular joints.
33861772	7	24	theme	human	1091:1095	arg1	SF					1097:1098	human SF	1091:1098	human SF	1091:1098	Importantly, equine SF contains about half of the PL content determined in human SF with some characteristic changes in PL composition.
33861772	1	25	theme	low	262:264	arg1	availability					266:277	low availability	262:277	low availability of SF from healthy articular joints	262:313	The current limitations in evaluating synovial fluid (SF) components in health and disease and between species are due in part to the lack of data on normal SF, because of low availability of SF from healthy articular joints.
33861772	2	26	theme	phospholipid	377:388	arg1	profiles					395:402	phospholipid (PL) profiles	377:402	phospholipid (PL) profiles of normal knee SF obtained from equine and human donors	377:458	Our study aimed to quantify species-dependent differences in phospholipid (PL) profiles of normal knee SF obtained from equine and human donors.
33861772	4	27	theme	Apo	691:693	arg1	B-100					696:700	apolipoprotein (Apo) B-100	675:700	apolipoprotein (Apo) B-100	675:700	PL species extracted from SF were quantitated by mass spectrometry whereas ELISA determined apolipoprotein (Apo) B-100.
33861772	4	28	theme	PL	583:584	arg1	species					586:592	PL species	583:592	PL species extracted from SF	583:610	PL species extracted from SF were quantitated by mass spectrometry whereas ELISA determined apolipoprotein (Apo) B-100.
33861772	6	29	theme	PL	867:868	arg1	species					870:876	89 PL species	864:876	89 PL species	864:876	Six lipid classes with 89 PL species were quantified, namely phosphatidylcholine, lysophosphatidylcholine, sphingomyelin, phosphatidylethanolamine, plasmalogen, and ceramide.
33861772	4	30	theme	apolipoprotein	675:688	arg1	B-100					696:700	apolipoprotein (Apo) B-100	675:700	apolipoprotein (Apo) B-100	675:700	PL species extracted from SF were quantitated by mass spectrometry whereas ELISA determined apolipoprotein (Apo) B-100.
33861772	2	31	theme	species-dependent	344:360	arg1	differences					362:372	species-dependent differences	344:372	species-dependent differences in phospholipid (PL) profiles of normal knee SF obtained from equine and human donors	344:458	Our study aimed to quantify species-dependent differences in phospholipid (PL) profiles of normal knee SF obtained from equine and human donors.
33861772	1	32	theme	data	232:235	arg1	lack					224:227	the lack	220:227	the lack of data on normal SF	220:248	The current limitations in evaluating synovial fluid (SF) components in health and disease and between species are due in part to the lack of data on normal SF, because of low availability of SF from healthy articular joints.
33861772	5	33	with	test	723:726	arg1	adjustment					733:742	adjustment	733:742	adjustment of scores for tied values	733:768	Wilcoxon's rank sum test with adjustment of scores for tied values was applied followed by Holm´s method to account for multiple testing.
33861772	5	34	theme	scores	747:752	arg1	adjustment					733:742	adjustment	733:742	adjustment of scores for tied values	733:768	Wilcoxon's rank sum test with adjustment of scores for tied values was applied followed by Holm´s method to account for multiple testing.
33861772	1	35	from	availability	266:277	arg1	joints					308:313	healthy articular joints	290:313	healthy articular joints	290:313	The current limitations in evaluating synovial fluid (SF) components in health and disease and between species are due in part to the lack of data on normal SF, because of low availability of SF from healthy articular joints.
33861772	8	36	theme	observed	1257:1264	arg1	profiles					1279:1286	the observed different PL profiles	1253:1286	the observed different PL profiles	1253:1286	Nutritional habits, decreased apolipoprotein levels and altered enzymatic activities may have caused the observed different PL profiles.
33861772	9	37	from	levels	1354:1359	arg1	SF					1393:1394	equine knee SF	1381:1394	equine knee SF	1381:1394	Our study provides comprehensive quantitative data on PL species levels in normal human and equine knee SF so that research in joint diseases and articular lubrication can be facilitated.
33861772	9	37	from	levels	1354:1359	arg1	human					1371:1375	human	1371:1375	human	1371:1375	Our study provides comprehensive quantitative data on PL species levels in normal human and equine knee SF so that research in joint diseases and articular lubrication can be facilitated.
33861772	5	38	dep	applied	774:780	arg1	followed					782:789	followed	782:789	followed by Holm´s method to account for multiple testing	782:838	Wilcoxon's rank sum test with adjustment of scores for tied values was applied followed by Holm´s method to account for multiple testing.
33861772	8	39	theme	enzymatic	1216:1224	arg1	activities					1226:1235	altered enzymatic activities	1208:1235	altered enzymatic activities	1208:1235	Nutritional habits, decreased apolipoprotein levels and altered enzymatic activities may have caused the observed different PL profiles.
33861772	8	40	theme	altered	1208:1214	arg1	activities					1226:1235	altered enzymatic activities	1208:1235	altered enzymatic activities	1208:1235	Nutritional habits, decreased apolipoprotein levels and altered enzymatic activities may have caused the observed different PL profiles.
33861772	7	41	theme	content	1069:1075	arg1	half					1054:1057	about half	1048:1057	about half of the PL content determined in human SF with some characteristic changes in PL composition	1048:1149	Importantly, equine SF contains about half of the PL content determined in human SF with some characteristic changes in PL composition.
33861772	1	42	theme	normal	240:245	arg1	SF					247:248	normal SF	240:248	normal SF	240:248	The current limitations in evaluating synovial fluid (SF) components in health and disease and between species are due in part to the lack of data on normal SF, because of low availability of SF from healthy articular joints.
33861772	1	43	from	part	212:215	arg1	limitations					102:112	The current limitations	90:112	The current limitations in evaluating synovial fluid (SF) components in health and disease and between species	90:199	The current limitations in evaluating synovial fluid (SF) components in health and disease and between species are due in part to the lack of data on normal SF, because of low availability of SF from healthy articular joints.
33861772	1	43	from	part	212:215	arg1	due					205:207	due	205:207	due	205:207	The current limitations in evaluating synovial fluid (SF) components in health and disease and between species are due in part to the lack of data on normal SF, because of low availability of SF from healthy articular joints.
33861772	9	44	theme	PL	1343:1344	arg1	levels					1354:1359	PL species levels	1343:1359	PL species levels in normal human and equine knee SF so that research in joint diseases and articular lubrication can be facilitated	1343:1474	Our study provides comprehensive quantitative data on PL species levels in normal human and equine knee SF so that research in joint diseases and articular lubrication can be facilitated.
33861772	9	45	from	research	1404:1411	arg1	diseases					1422:1429	joint diseases	1416:1429	joint diseases	1416:1429	Our study provides comprehensive quantitative data on PL species levels in normal human and equine knee SF so that research in joint diseases and articular lubrication can be facilitated.
33861772	9	45	from	research	1404:1411	arg1	lubrication					1445:1455	articular lubrication	1435:1455	articular lubrication	1435:1455	Our study provides comprehensive quantitative data on PL species levels in normal human and equine knee SF so that research in joint diseases and articular lubrication can be facilitated.
33861772	5	46	theme	tied	758:761	arg1	values					763:768	tied values	758:768	tied values	758:768	Wilcoxon's rank sum test with adjustment of scores for tied values was applied followed by Holm´s method to account for multiple testing.
33861772	0	47	theme	normal	39:44	arg1	fluid					60:64	normal knee synovial fluid	39:64	normal knee synovial fluid from humans and horses	39:87	A comparative study on the lipidome of normal knee synovial fluid from humans and horses.
33861772	6	48	theme	lipid	845:849	arg1	classes					851:857	Six lipid classes	841:857	Six lipid classes with 89 PL species	841:876	Six lipid classes with 89 PL species were quantified, namely phosphatidylcholine, lysophosphatidylcholine, sphingomyelin, phosphatidylethanolamine, plasmalogen, and ceramide.
33861772	2	49	theme	SF	419:420	arg1	profiles					395:402	phospholipid (PL) profiles	377:402	phospholipid (PL) profiles of normal knee SF obtained from equine and human donors	377:458	Our study aimed to quantify species-dependent differences in phospholipid (PL) profiles of normal knee SF obtained from equine and human donors.
33861772	2	50	from	differences	362:372	arg1	profiles					395:402	phospholipid (PL) profiles	377:402	phospholipid (PL) profiles of normal knee SF obtained from equine and human donors	377:458	Our study aimed to quantify species-dependent differences in phospholipid (PL) profiles of normal knee SF obtained from equine and human donors.
33861772	1	51	theme	healthy	290:296	arg1	joints					308:313	healthy articular joints	290:313	healthy articular joints	290:313	The current limitations in evaluating synovial fluid (SF) components in health and disease and between species are due in part to the lack of data on normal SF, because of low availability of SF from healthy articular joints.
33861772	9	52	theme	joint	1416:1420	arg1	diseases					1422:1429	joint diseases	1416:1429	joint diseases	1416:1429	Our study provides comprehensive quantitative data on PL species levels in normal human and equine knee SF so that research in joint diseases and articular lubrication can be facilitated.
33861772	5	53	theme	rank	714:717	arg1	test					723:726	Wilcoxon's rank sum test	703:726	Wilcoxon's rank sum test with adjustment of scores for tied values	703:768	Wilcoxon's rank sum test with adjustment of scores for tied values was applied followed by Holm´s method to account for multiple testing.
33861772	9	54	theme	comprehensive	1308:1320	arg1	data					1335:1338	comprehensive quantitative data	1308:1338	comprehensive quantitative data on PL species levels in normal human and equine knee SF so that research in joint diseases and articular lubrication can be facilitated	1308:1474	Our study provides comprehensive quantitative data on PL species levels in normal human and equine knee SF so that research in joint diseases and articular lubrication can be facilitated.
33861772	1	55	from	due	205:207	arg1	part					212:215	part	212:215	part	212:215	The current limitations in evaluating synovial fluid (SF) components in health and disease and between species are due in part to the lack of data on normal SF, because of low availability of SF from healthy articular joints.
33861772	7	56	theme	equine	1029:1034	arg1	SF					1036:1037	equine SF	1029:1037	equine SF	1029:1037	Importantly, equine SF contains about half of the PL content determined in human SF with some characteristic changes in PL composition.
33861772	1	57	theme	current	94:100	arg1	limitations					102:112	The current limitations	90:112	The current limitations in evaluating synovial fluid (SF) components in health and disease and between species	90:199	The current limitations in evaluating synovial fluid (SF) components in health and disease and between species are due in part to the lack of data on normal SF, because of low availability of SF from healthy articular joints.
33861772	1	57	theme	current	94:100	arg1	due					205:207	due	205:207	due	205:207	The current limitations in evaluating synovial fluid (SF) components in health and disease and between species are due in part to the lack of data on normal SF, because of low availability of SF from healthy articular joints.
33861772	9	58	theme	quantitative	1322:1333	arg1	data					1335:1338	comprehensive quantitative data	1308:1338	comprehensive quantitative data on PL species levels in normal human and equine knee SF so that research in joint diseases and articular lubrication can be facilitated	1308:1474	Our study provides comprehensive quantitative data on PL species levels in normal human and equine knee SF so that research in joint diseases and articular lubrication can be facilitated.
33861772	9	59	from	data	1335:1338	arg1	levels					1354:1359	PL species levels	1343:1359	PL species levels in normal human and equine knee SF so that research in joint diseases and articular lubrication can be facilitated	1343:1474	Our study provides comprehensive quantitative data on PL species levels in normal human and equine knee SF so that research in joint diseases and articular lubrication can be facilitated.
33861772	0	60	from	horses	82:87	arg1	fluid					60:64	normal knee synovial fluid	39:64	normal knee synovial fluid from humans and horses	39:87	A comparative study on the lipidome of normal knee synovial fluid from humans and horses.
33861772	0	60	from	horses	82:87	arg1	lipidome					27:34	the lipidome	23:34	the lipidome of normal knee synovial fluid from humans and horses	23:87	A comparative study on the lipidome of normal knee synovial fluid from humans and horses.
33861772	5	61	theme	multiple	823:830	arg1	testing					832:838	multiple testing	823:838	multiple testing	823:838	Wilcoxon's rank sum test with adjustment of scores for tied values was applied followed by Holm´s method to account for multiple testing.
33861772	6	62	with	classes	851:857	arg1	species					870:876	89 PL species	864:876	89 PL species	864:876	Six lipid classes with 89 PL species were quantified, namely phosphatidylcholine, lysophosphatidylcholine, sphingomyelin, phosphatidylethanolamine, plasmalogen, and ceramide.
33861772	9	63	theme	equine	1381:1386	arg1	SF					1393:1394	equine knee SF	1381:1394	equine knee SF	1381:1394	Our study provides comprehensive quantitative data on PL species levels in normal human and equine knee SF so that research in joint diseases and articular lubrication can be facilitated.
33861772	3	64	theme	human	544:548	arg1	donors					561:566	15 and 13 joint-healthy human and equine donors	520:566	15 and 13 joint-healthy human and equine donors	520:566	Knee SF was obtained during autopsy by arthrocentesis from 15 and 13 joint-healthy human and equine donors, respectively.
33861772	1	65	from	components	148:157	arg1	disease					173:179	disease	173:179	disease	173:179	The current limitations in evaluating synovial fluid (SF) components in health and disease and between species are due in part to the lack of data on normal SF, because of low availability of SF from healthy articular joints.
33861772	1	65	from	components	148:157	arg1	health					162:167	health	162:167	health	162:167	The current limitations in evaluating synovial fluid (SF) components in health and disease and between species are due in part to the lack of data on normal SF, because of low availability of SF from healthy articular joints.
33861772	4	66	theme	mass	632:635	arg1	spectrometry					637:648	mass spectrometry	632:648	mass spectrometry	632:648	PL species extracted from SF were quantitated by mass spectrometry whereas ELISA determined apolipoprotein (Apo) B-100.
33861772	8	67	theme	PL	1276:1277	arg1	profiles					1279:1286	the observed different PL profiles	1253:1286	the observed different PL profiles	1253:1286	Nutritional habits, decreased apolipoprotein levels and altered enzymatic activities may have caused the observed different PL profiles.
33861772	0	68	theme	synovial	51:58	arg1	fluid					60:64	normal knee synovial fluid	39:64	normal knee synovial fluid from humans and horses	39:87	A comparative study on the lipidome of normal knee synovial fluid from humans and horses.
33861772	0	69	from	humans	71:76	arg1	fluid					60:64	normal knee synovial fluid	39:64	normal knee synovial fluid from humans and horses	39:87	A comparative study on the lipidome of normal knee synovial fluid from humans and horses.
33861772	0	69	from	humans	71:76	arg1	lipidome					27:34	the lipidome	23:34	the lipidome of normal knee synovial fluid from humans and horses	23:87	A comparative study on the lipidome of normal knee synovial fluid from humans and horses.
33861772	5	70	theme	Holm´s	794:799	arg1	method					801:806	Holm´s method	794:806	Holm´s method	794:806	Wilcoxon's rank sum test with adjustment of scores for tied values was applied followed by Holm´s method to account for multiple testing.
33861772	8	71	theme	Nutritional	1152:1162	arg1	habits					1164:1169	Nutritional habits	1152:1169	Nutritional habits	1152:1169	Nutritional habits, decreased apolipoprotein levels and altered enzymatic activities may have caused the observed different PL profiles.
33861772	8	72	theme	apolipoprotein	1182:1195	arg1	levels					1197:1202	decreased apolipoprotein levels	1172:1202	decreased apolipoprotein levels	1172:1202	Nutritional habits, decreased apolipoprotein levels and altered enzymatic activities may have caused the observed different PL profiles.
33861772	0	73	theme	knee	46:49	arg1	fluid					60:64	normal knee synovial fluid	39:64	normal knee synovial fluid from humans and horses	39:87	A comparative study on the lipidome of normal knee synovial fluid from humans and horses.
33861772	9	74	theme	knee	1388:1391	arg1	SF					1393:1394	equine knee SF	1381:1394	equine knee SF	1381:1394	Our study provides comprehensive quantitative data on PL species levels in normal human and equine knee SF so that research in joint diseases and articular lubrication can be facilitated.
33861772	7	75	from	changes	1125:1131	arg1	composition					1139:1149	PL composition	1136:1149	PL composition	1136:1149	Importantly, equine SF contains about half of the PL content determined in human SF with some characteristic changes in PL composition.
33861772	1	76	theme	synovial	128:135	arg1	SF					144:145	SF	144:145	SF	144:145	The current limitations in evaluating synovial fluid (SF) components in health and disease and between species are due in part to the lack of data on normal SF, because of low availability of SF from healthy articular joints.
33861772	1	76	theme	synovial	128:135	arg1	fluid					137:141	synovial fluid	128:141	synovial fluid (SF) components in health and disease	128:179	The current limitations in evaluating synovial fluid (SF) components in health and disease and between species are due in part to the lack of data on normal SF, because of low availability of SF from healthy articular joints.
33861772	7	77	contain	contains	1039:1046	arg2	half					1054:1057	about half	1048:1057	about half of the PL content determined in human SF with some characteristic changes in PL composition	1048:1149	Importantly, equine SF contains about half of the PL content determined in human SF with some characteristic changes in PL composition.
33861772	7	77	contain	contains	1039:1046	arg1	SF					1036:1037	equine SF	1029:1037	equine SF	1029:1037	Importantly, equine SF contains about half of the PL content determined in human SF with some characteristic changes in PL composition.
33861772	9	78	theme	species	1346:1352	arg1	levels					1354:1359	PL species levels	1343:1359	PL species levels in normal human and equine knee SF so that research in joint diseases and articular lubrication can be facilitated	1343:1474	Our study provides comprehensive quantitative data on PL species levels in normal human and equine knee SF so that research in joint diseases and articular lubrication can be facilitated.
32673713	11	0	dep	%	1474:1474	arg1	97-99					1469:1473	97-99	1469:1473	97-99	1469:1473	A reduction of 4-NP up to 97-99% was achieved by simply passing it through column packed with catalyst under gravity.
32673713	11	1	theme	4-NP	1458:1461	arg1	reduction					1445:1453	A reduction	1443:1453	A reduction of 4-NP up to 97-99%	1443:1474	A reduction of 4-NP up to 97-99% was achieved by simply passing it through column packed with catalyst under gravity.
32673713	8	2	used	used	1068:1071	arg2	Cu0/Alg-CNBs					1051:1062	Cu0/Alg-CNBs	1051:1062	Cu0/Alg-CNBs	1051:1062	Cu0/Alg-CNBs was used further for the reduction of methyl orange (MO), congo red (CR), 2-nitrophenol (2-NP) and 2,6-dinitrophenol (2,6-DNP).
32673713	13	3	theme	reaction	1828:1835	arg1	completion					1810:1819	completion	1810:1819	completion of the reaction	1810:1835	Also, the prepared catalyst beads can be easily prepared, recovered simply by discarding the dye after completion of the reaction, recyclable and its good catalytic activity.
32673713	4	4	theme	different	655:663	arg1	dyes					673:676	different organic dyes	655:676	different organic dyes	655:676	The surface coated transition metal ions on Alg-CNBs were reduced to zero-valent state (M0), through strong reducing agent NaBH4, and used for the catalytic reduction of different organic dyes.
32673713	10	5	theme	large	1294:1298	arg1	scale					1300:1304	large scale	1294:1304	large scale	1294:1304	For large scale and continuous reduction of 4-NP to 4-AP, the column was packed with 0.15 g catalyst and the reducible reactants were passed through it.
32673713	4	6	theme	coated	497:502	arg1	ions					521:524	The surface coated transition metal ions	485:524	The surface coated transition metal ions on Alg-CNBs	485:536	The surface coated transition metal ions on Alg-CNBs were reduced to zero-valent state (M0), through strong reducing agent NaBH4, and used for the catalytic reduction of different organic dyes.
32673713	13	7	dep	beads	1735:1739	arg1	prepared					1755:1762	prepared	1755:1762	can be easily prepared	1741:1762	Also, the prepared catalyst beads can be easily prepared, recovered simply by discarding the dye after completion of the reaction, recyclable and its good catalytic activity.
32673713	4	8	theme	zero-valent	554:564	arg1	M0					573:574	M0	573:574	M0	573:574	The surface coated transition metal ions on Alg-CNBs were reduced to zero-valent state (M0), through strong reducing agent NaBH4, and used for the catalytic reduction of different organic dyes.
32673713	4	8	theme	zero-valent	554:564	arg1	state					566:570	zero-valent state	554:570	zero-valent state (M0)	554:575	The surface coated transition metal ions on Alg-CNBs were reduced to zero-valent state (M0), through strong reducing agent NaBH4, and used for the catalytic reduction of different organic dyes.
32673713	5	9	theme	surface	683:689	arg1	morphology					691:700	The surface morphology	679:700	The surface morphology of all catalysts	679:717	The surface morphology of all catalysts was investigated by FE-SEM and metal uptake capacity was investigated by ICP-OES.
32673713	4	10	theme	surface	489:495	arg1	ions					521:524	The surface coated transition metal ions	485:524	The surface coated transition metal ions on Alg-CNBs	485:536	The surface coated transition metal ions on Alg-CNBs were reduced to zero-valent state (M0), through strong reducing agent NaBH4, and used for the catalytic reduction of different organic dyes.
32673713	4	11	theme	catalytic	632:640	arg1	reduction					642:650	the catalytic reduction	628:650	the catalytic reduction of different organic dyes	628:676	The surface coated transition metal ions on Alg-CNBs were reduced to zero-valent state (M0), through strong reducing agent NaBH4, and used for the catalytic reduction of different organic dyes.
32673713	2	12	theme	carbon	270:275	arg1	beads					285:289	The alginate carbon nitride beads	257:289	The alginate carbon nitride beads (Alg-CNBs)	257:300	The alginate carbon nitride beads (Alg-CNBs) were prepared by introducing the g-C3N4 dispersed Alg aqueous solution to a divalent Ca2+ ions solution.
32673713	2	12	theme	carbon	270:275	arg1	Alg-CNBs					292:299	Alg-CNBs	292:299	Alg-CNBs	292:299	The alginate carbon nitride beads (Alg-CNBs) were prepared by introducing the g-C3N4 dispersed Alg aqueous solution to a divalent Ca2+ ions solution.
32673713	10	13	theme	reducible	1399:1407	arg1	reactants					1409:1417	the reducible reactants	1395:1417	the reducible reactants	1395:1417	For large scale and continuous reduction of 4-NP to 4-AP, the column was packed with 0.15 g catalyst and the reducible reactants were passed through it.
32673713	4	14	theme	reducing	593:600	arg1	NaBH4					608:612	strong reducing agent NaBH4	586:612	strong reducing agent NaBH4	586:612	The surface coated transition metal ions on Alg-CNBs were reduced to zero-valent state (M0), through strong reducing agent NaBH4, and used for the catalytic reduction of different organic dyes.
32673713	2	15	theme	alginate	261:268	arg1	beads					285:289	The alginate carbon nitride beads	257:289	The alginate carbon nitride beads (Alg-CNBs)	257:300	The alginate carbon nitride beads (Alg-CNBs) were prepared by introducing the g-C3N4 dispersed Alg aqueous solution to a divalent Ca2+ ions solution.
32673713	2	15	theme	alginate	261:268	arg1	Alg-CNBs					292:299	Alg-CNBs	292:299	Alg-CNBs	292:299	The alginate carbon nitride beads (Alg-CNBs) were prepared by introducing the g-C3N4 dispersed Alg aqueous solution to a divalent Ca2+ ions solution.
32673713	12	16	theme	reduction	1688:1696	arg1	process					1698:1704	the reduction process	1684:1704	the reduction process	1684:1704	Similarly, effects of changing initial dye concentration, catalytic dosage and NaBH4 performance has been investigated for the reduction process.
32673713	0	17	theme	organic	120:126	arg1	pollutants					128:137	organic pollutants	120:137	organic pollutants	120:137	Metal nanoparticles decorated sodium alginate‑carbon nitride composite beads as effective catalyst for the reduction of organic pollutants.
32673713	4	18	theme	dyes	673:676	arg1	reduction					642:650	the catalytic reduction	628:650	the catalytic reduction of different organic dyes	628:676	The surface coated transition metal ions on Alg-CNBs were reduced to zero-valent state (M0), through strong reducing agent NaBH4, and used for the catalytic reduction of different organic dyes.
32673713	2	19	theme	Ca2+	387:390	arg1	ions					392:395	divalent Ca2+ ions	378:395	a divalent Ca2+ ions solution	376:404	The alginate carbon nitride beads (Alg-CNBs) were prepared by introducing the g-C3N4 dispersed Alg aqueous solution to a divalent Ca2+ ions solution.
32673713	10	20	theme	0.15 g	1375:1380	arg1	catalyst					1382:1389	0.15 g catalyst	1375:1389	0.15 g catalyst	1375:1389	For large scale and continuous reduction of 4-NP to 4-AP, the column was packed with 0.15 g catalyst and the reducible reactants were passed through it.
32673713	5	21	theme	uptake	756:761	arg1	capacity					763:770	metal uptake capacity	750:770	metal uptake capacity	750:770	The surface morphology of all catalysts was investigated by FE-SEM and metal uptake capacity was investigated by ICP-OES.
32673713	10	22	theme	continuous	1310:1319	arg1	reduction					1321:1329	continuous reduction	1310:1329	continuous reduction of 4-NP to 4-AP	1310:1345	For large scale and continuous reduction of 4-NP to 4-AP, the column was packed with 0.15 g catalyst and the reducible reactants were passed through it.
32673713	4	23	theme	agent	602:606	arg1	NaBH4					608:612	strong reducing agent NaBH4	586:612	strong reducing agent NaBH4	586:612	The surface coated transition metal ions on Alg-CNBs were reduced to zero-valent state (M0), through strong reducing agent NaBH4, and used for the catalytic reduction of different organic dyes.
32673713	8	24	theme	methyl	1102:1107	arg1	MO					1117:1118	MO	1117:1118	MO	1117:1118	Cu0/Alg-CNBs was used further for the reduction of methyl orange (MO), congo red (CR), 2-nitrophenol (2-NP) and 2,6-dinitrophenol (2,6-DNP).
32673713	8	24	theme	methyl	1102:1107	arg1	orange					1109:1114	methyl orange	1102:1114	methyl orange (MO)	1102:1119	Cu0/Alg-CNBs was used further for the reduction of methyl orange (MO), congo red (CR), 2-nitrophenol (2-NP) and 2,6-dinitrophenol (2,6-DNP).
32673713	8	25	theme	orange	1109:1114	arg1	reduction					1089:1097	the reduction	1085:1097	the reduction of methyl orange (MO), congo red (CR), 2-nitrophenol (2-NP) and 2,6-dinitrophenol (2,6-DNP)	1085:1189	Cu0/Alg-CNBs was used further for the reduction of methyl orange (MO), congo red (CR), 2-nitrophenol (2-NP) and 2,6-dinitrophenol (2,6-DNP).
32673713	13	26	theme	good	1857:1860	arg1	activity					1872:1879	its good catalytic activity	1853:1879	its good catalytic activity	1853:1879	Also, the prepared catalyst beads can be easily prepared, recovered simply by discarding the dye after completion of the reaction, recyclable and its good catalytic activity.
32673713	5	27	theme	catalysts	709:717	arg1	morphology					691:700	The surface morphology	679:700	The surface morphology of all catalysts	679:717	The surface morphology of all catalysts was investigated by FE-SEM and metal uptake capacity was investigated by ICP-OES.
32673713	2	28	theme	aqueous	356:362	arg1	solution					364:371	the g-C3N4 dispersed Alg aqueous solution	331:371	the g-C3N4 dispersed Alg aqueous solution to a divalent Ca2+ ions solution	331:404	The alginate carbon nitride beads (Alg-CNBs) were prepared by introducing the g-C3N4 dispersed Alg aqueous solution to a divalent Ca2+ ions solution.
32673713	0	29	theme	Metal	0:4	arg1	nanoparticles					6:18	Metal nanoparticles	0:18	Metal nanoparticles	0:18	Metal nanoparticles decorated sodium alginate‑carbon nitride composite beads as effective catalyst for the reduction of organic pollutants.
32673713	8	30	theme	2-nitrophenol	1138:1150	arg1	reduction					1089:1097	the reduction	1085:1097	the reduction of methyl orange (MO), congo red (CR), 2-nitrophenol (2-NP) and 2,6-dinitrophenol (2,6-DNP)	1085:1189	Cu0/Alg-CNBs was used further for the reduction of methyl orange (MO), congo red (CR), 2-nitrophenol (2-NP) and 2,6-dinitrophenol (2,6-DNP).
32673713	2	31	theme	Alg	352:354	arg1	solution					364:371	the g-C3N4 dispersed Alg aqueous solution	331:371	the g-C3N4 dispersed Alg aqueous solution to a divalent Ca2+ ions solution	331:404	The alginate carbon nitride beads (Alg-CNBs) were prepared by introducing the g-C3N4 dispersed Alg aqueous solution to a divalent Ca2+ ions solution.
32673713	0	32	theme	sodium	30:35	arg1	beads					71:75	sodium alginate‑carbon nitride composite beads	30:75	sodium alginate‑carbon nitride composite beads	30:75	Metal nanoparticles decorated sodium alginate‑carbon nitride composite beads as effective catalyst for the reduction of organic pollutants.
32673713	6	33	theme	4-nitrophenol	908:920	arg1	reduction					895:903	the reduction	891:903	the reduction of 4-nitrophenol (4-NP)	891:927	The prepared M0/Alg-CNBs (where M0 = Ag0, Ni0, Fe0 and Cu0) catalysts were tested against the reduction of 4-nitrophenol (4-NP) to recognize the fastest one.
32673713	8	34	theme	2,6-dinitrophenol	1163:1179	arg1	reduction					1089:1097	the reduction	1085:1097	the reduction of methyl orange (MO), congo red (CR), 2-nitrophenol (2-NP) and 2,6-dinitrophenol (2,6-DNP)	1085:1189	Cu0/Alg-CNBs was used further for the reduction of methyl orange (MO), congo red (CR), 2-nitrophenol (2-NP) and 2,6-dinitrophenol (2,6-DNP).
32673713	2	35	theme	ions	392:395	arg1	solution					397:404	a divalent Ca2+ ions solution	376:404	a divalent Ca2+ ions solution	376:404	The alginate carbon nitride beads (Alg-CNBs) were prepared by introducing the g-C3N4 dispersed Alg aqueous solution to a divalent Ca2+ ions solution.
32673713	12	36	theme	initial	1592:1598	arg1	concentration					1604:1616	initial dye concentration	1592:1616	initial dye concentration	1592:1616	Similarly, effects of changing initial dye concentration, catalytic dosage and NaBH4 performance has been investigated for the reduction process.
32673713	0	37	theme	pollutants	128:137	arg1	reduction					107:115	the reduction	103:115	the reduction of organic pollutants	103:137	Metal nanoparticles decorated sodium alginate‑carbon nitride composite beads as effective catalyst for the reduction of organic pollutants.
32673713	2	38	theme	nitride	277:283	arg1	beads					285:289	The alginate carbon nitride beads	257:289	The alginate carbon nitride beads (Alg-CNBs)	257:300	The alginate carbon nitride beads (Alg-CNBs) were prepared by introducing the g-C3N4 dispersed Alg aqueous solution to a divalent Ca2+ ions solution.
32673713	2	38	theme	nitride	277:283	arg1	Alg-CNBs					292:299	Alg-CNBs	292:299	Alg-CNBs	292:299	The alginate carbon nitride beads (Alg-CNBs) were prepared by introducing the g-C3N4 dispersed Alg aqueous solution to a divalent Ca2+ ions solution.
32673713	11	39	dep	reduction	1445:1453	arg1	%					1474:1474	%	1474:1474	%	1474:1474	A reduction of 4-NP up to 97-99% was achieved by simply passing it through column packed with catalyst under gravity.
32673713	1	40	theme	nitride	198:204	arg1	fabrication					176:186	fabrication	176:186	fabrication of carbon nitride (g-C3N4) incorporated sodium alginate (Alg) beads	176:254	Herein, a simple method is used for fabrication of carbon nitride (g-C3N4) incorporated sodium alginate (Alg) beads.
32673713	0	41	theme	nitride	53:59	arg1	beads					71:75	sodium alginate‑carbon nitride composite beads	30:75	sodium alginate‑carbon nitride composite beads	30:75	Metal nanoparticles decorated sodium alginate‑carbon nitride composite beads as effective catalyst for the reduction of organic pollutants.
32673713	11	42	dep	97-99	1469:1473	arg1	to					1466:1467	to	1466:1467	to	1466:1467	A reduction of 4-NP up to 97-99% was achieved by simply passing it through column packed with catalyst under gravity.
32673713	10	43	theme	4-NP	1334:1337	arg1	scale					1300:1304	large scale	1294:1304	large scale	1294:1304	For large scale and continuous reduction of 4-NP to 4-AP, the column was packed with 0.15 g catalyst and the reducible reactants were passed through it.
32673713	10	43	theme	4-NP	1334:1337	arg1	reduction					1321:1329	continuous reduction	1310:1329	continuous reduction of 4-NP to 4-AP	1310:1345	For large scale and continuous reduction of 4-NP to 4-AP, the column was packed with 0.15 g catalyst and the reducible reactants were passed through it.
32673713	13	44	theme	prepared	1717:1724	arg1	beads					1735:1739	the prepared catalyst beads	1713:1739	the prepared catalyst beads	1713:1739	Also, the prepared catalyst beads can be easily prepared, recovered simply by discarding the dye after completion of the reaction, recyclable and its good catalytic activity.
32673713	2	45	theme	divalent	378:385	arg1	ions					392:395	divalent Ca2+ ions	378:395	a divalent Ca2+ ions solution	376:404	The alginate carbon nitride beads (Alg-CNBs) were prepared by introducing the g-C3N4 dispersed Alg aqueous solution to a divalent Ca2+ ions solution.
32673713	0	46	theme	alginate‑carbon	37:51	arg1	beads					71:75	sodium alginate‑carbon nitride composite beads	30:75	sodium alginate‑carbon nitride composite beads	30:75	Metal nanoparticles decorated sodium alginate‑carbon nitride composite beads as effective catalyst for the reduction of organic pollutants.
32673713	4	47	theme	metal	515:519	arg1	ions					521:524	The surface coated transition metal ions	485:524	The surface coated transition metal ions on Alg-CNBs	485:536	The surface coated transition metal ions on Alg-CNBs were reduced to zero-valent state (M0), through strong reducing agent NaBH4, and used for the catalytic reduction of different organic dyes.
32673713	4	48	from	ions	521:524	arg1	Alg-CNBs					529:536	Alg-CNBs	529:536	Alg-CNBs	529:536	The surface coated transition metal ions on Alg-CNBs were reduced to zero-valent state (M0), through strong reducing agent NaBH4, and used for the catalytic reduction of different organic dyes.
32673713	4	49	theme	strong	586:591	arg1	NaBH4					608:612	strong reducing agent NaBH4	586:612	strong reducing agent NaBH4	586:612	The surface coated transition metal ions on Alg-CNBs were reduced to zero-valent state (M0), through strong reducing agent NaBH4, and used for the catalytic reduction of different organic dyes.
32673713	9	50	theme	rate	1229:1232	arg1	constant					1234:1241	rate constant	1229:1241	rate constant of 0.135, 0.077 and 0.384 min-1, respectively	1229:1287	The MO, CR and 4-NP was reduced with rate constant of 0.135, 0.077 and 0.384 min-1, respectively.
32673713	5	51	theme	metal	750:754	arg1	capacity					763:770	metal uptake capacity	750:770	metal uptake capacity	750:770	The surface morphology of all catalysts was investigated by FE-SEM and metal uptake capacity was investigated by ICP-OES.
32673713	4	52	theme	transition	504:513	arg1	ions					521:524	The surface coated transition metal ions	485:524	The surface coated transition metal ions on Alg-CNBs	485:536	The surface coated transition metal ions on Alg-CNBs were reduced to zero-valent state (M0), through strong reducing agent NaBH4, and used for the catalytic reduction of different organic dyes.
32673713	6	53	theme	M0/Alg-CNBs	814:824	arg1	catalysts					861:869	The prepared M0/Alg-CNBs (where M0 = Ag0, Ni0, Fe0 and Cu0) catalysts	801:869	The prepared M0/Alg-CNBs (where M0 = Ag0, Ni0, Fe0 and Cu0) catalysts	801:869	The prepared M0/Alg-CNBs (where M0 = Ag0, Ni0, Fe0 and Cu0) catalysts were tested against the reduction of 4-nitrophenol (4-NP) to recognize the fastest one.
32673713	12	54	theme	catalytic	1619:1627	arg1	dosage					1629:1634	catalytic dosage	1619:1634	catalytic dosage	1619:1634	Similarly, effects of changing initial dye concentration, catalytic dosage and NaBH4 performance has been investigated for the reduction process.
32673713	3	55	theme	salt	469:472	arg1	solutions					474:482	different transition metal salt solutions	442:482	different transition metal salt solutions	442:482	Alg-CNBs were further treated with different transition metal salt solutions.
32673713	0	56	theme	composite	61:69	arg1	beads					71:75	sodium alginate‑carbon nitride composite beads	30:75	sodium alginate‑carbon nitride composite beads	30:75	Metal nanoparticles decorated sodium alginate‑carbon nitride composite beads as effective catalyst for the reduction of organic pollutants.
32673713	6	57	theme	prepared	805:812	arg1	M0/Alg-CNBs					814:824	The prepared M0/Alg-CNBs	801:824	The prepared M0/Alg-CNBs (where M0 = Ag0, Ni0, Fe0 and Cu0) catalysts	801:869	The prepared M0/Alg-CNBs (where M0 = Ag0, Ni0, Fe0 and Cu0) catalysts were tested against the reduction of 4-nitrophenol (4-NP) to recognize the fastest one.
32673713	12	58	theme	NaBH4	1640:1644	arg1	performance					1646:1656	NaBH4 performance	1640:1656	NaBH4 performance	1640:1656	Similarly, effects of changing initial dye concentration, catalytic dosage and NaBH4 performance has been investigated for the reduction process.
32673713	4	59	theme	organic	665:671	arg1	dyes					673:676	different organic dyes	655:676	different organic dyes	655:676	The surface coated transition metal ions on Alg-CNBs were reduced to zero-valent state (M0), through strong reducing agent NaBH4, and used for the catalytic reduction of different organic dyes.
32673713	6	60	dep	M0/Alg-CNBs	814:824	arg1	Cu0					856:858	Cu0	856:858	Cu0	856:858	The prepared M0/Alg-CNBs (where M0 = Ag0, Ni0, Fe0 and Cu0) catalysts were tested against the reduction of 4-nitrophenol (4-NP) to recognize the fastest one.
32673713	6	60	dep	M0/Alg-CNBs	814:824	arg1	Fe0					848:850	Fe0	848:850	Fe0	848:850	The prepared M0/Alg-CNBs (where M0 = Ag0, Ni0, Fe0 and Cu0) catalysts were tested against the reduction of 4-nitrophenol (4-NP) to recognize the fastest one.
32673713	6	60	dep	M0/Alg-CNBs	814:824	arg1	Ni0					843:845	Ni0	843:845	Ni0	843:845	The prepared M0/Alg-CNBs (where M0 = Ag0, Ni0, Fe0 and Cu0) catalysts were tested against the reduction of 4-nitrophenol (4-NP) to recognize the fastest one.
32673713	3	61	dep	different	442:450	arg1	metal					463:467	metal	463:467	metal	463:467	Alg-CNBs were further treated with different transition metal salt solutions.
32673713	4	62	used	used	619:622	arg2	Alg-CNBs					529:536	Alg-CNBs	529:536	Alg-CNBs	529:536	The surface coated transition metal ions on Alg-CNBs were reduced to zero-valent state (M0), through strong reducing agent NaBH4, and used for the catalytic reduction of different organic dyes.
32673713	4	62	used	used	619:622	arg2	ions					521:524	The surface coated transition metal ions	485:524	The surface coated transition metal ions on Alg-CNBs	485:536	The surface coated transition metal ions on Alg-CNBs were reduced to zero-valent state (M0), through strong reducing agent NaBH4, and used for the catalytic reduction of different organic dyes.
32673713	13	63	theme	catalyst	1726:1733	arg1	beads					1735:1739	the prepared catalyst beads	1713:1739	the prepared catalyst beads	1713:1739	Also, the prepared catalyst beads can be easily prepared, recovered simply by discarding the dye after completion of the reaction, recyclable and its good catalytic activity.
32673713	9	64	theme	0.135	1246:1250	arg1	constant					1234:1241	rate constant	1229:1241	rate constant of 0.135, 0.077 and 0.384 min-1, respectively	1229:1287	The MO, CR and 4-NP was reduced with rate constant of 0.135, 0.077 and 0.384 min-1, respectively.
32673713	1	65	theme	simple	150:155	arg1	method					157:162	a simple method	148:162	a simple method	148:162	Herein, a simple method is used for fabrication of carbon nitride (g-C3N4) incorporated sodium alginate (Alg) beads.
32673713	3	66	theme	different	442:450	arg1	solutions					474:482	different transition metal salt solutions	442:482	different transition metal salt solutions	442:482	Alg-CNBs were further treated with different transition metal salt solutions.
32673713	12	67	theme	dye	1600:1602	arg1	concentration					1604:1616	initial dye concentration	1592:1616	initial dye concentration	1592:1616	Similarly, effects of changing initial dye concentration, catalytic dosage and NaBH4 performance has been investigated for the reduction process.
32673713	0	68	theme	effective	80:88	arg1	catalyst					90:97	effective catalyst	80:97	effective catalyst for the reduction of organic pollutants	80:137	Metal nanoparticles decorated sodium alginate‑carbon nitride composite beads as effective catalyst for the reduction of organic pollutants.
32673713	1	69	theme	carbon	191:196	arg1	nitride					198:204	carbon nitride	191:204	carbon nitride (g-C3N4) incorporated sodium alginate (Alg) beads	191:254	Herein, a simple method is used for fabrication of carbon nitride (g-C3N4) incorporated sodium alginate (Alg) beads.
32673713	1	69	theme	carbon	191:196	arg1	g-C3N4					207:212	g-C3N4	207:212	g-C3N4	207:212	Herein, a simple method is used for fabrication of carbon nitride (g-C3N4) incorporated sodium alginate (Alg) beads.
32673713	2	70	theme	dispersed	342:350	arg1	solution					364:371	the g-C3N4 dispersed Alg aqueous solution	331:371	the g-C3N4 dispersed Alg aqueous solution to a divalent Ca2+ ions solution	331:404	The alginate carbon nitride beads (Alg-CNBs) were prepared by introducing the g-C3N4 dispersed Alg aqueous solution to a divalent Ca2+ ions solution.
32673713	1	71	theme	sodium	228:233	arg1	Alg					245:247	Alg	245:247	Alg	245:247	Herein, a simple method is used for fabrication of carbon nitride (g-C3N4) incorporated sodium alginate (Alg) beads.
32673713	1	71	theme	sodium	228:233	arg1	beads					250:254	sodium alginate (Alg) beads	228:254	sodium alginate (Alg) beads	228:254	Herein, a simple method is used for fabrication of carbon nitride (g-C3N4) incorporated sodium alginate (Alg) beads.
32673713	8	72	theme	red	1128:1130	arg1	reduction					1089:1097	the reduction	1085:1097	the reduction of methyl orange (MO), congo red (CR), 2-nitrophenol (2-NP) and 2,6-dinitrophenol (2,6-DNP)	1085:1189	Cu0/Alg-CNBs was used further for the reduction of methyl orange (MO), congo red (CR), 2-nitrophenol (2-NP) and 2,6-dinitrophenol (2,6-DNP).
32673713	13	73	theme	catalytic	1862:1870	arg1	activity					1872:1879	its good catalytic activity	1853:1879	its good catalytic activity	1853:1879	Also, the prepared catalyst beads can be easily prepared, recovered simply by discarding the dye after completion of the reaction, recyclable and its good catalytic activity.
32673713	1	74	used	used	167:170	arg2	method					157:162	a simple method	148:162	a simple method	148:162	Herein, a simple method is used for fabrication of carbon nitride (g-C3N4) incorporated sodium alginate (Alg) beads.
32673713	2	75	theme	g-C3N4	335:340	arg1	solution					364:371	the g-C3N4 dispersed Alg aqueous solution	331:371	the g-C3N4 dispersed Alg aqueous solution to a divalent Ca2+ ions solution	331:404	The alginate carbon nitride beads (Alg-CNBs) were prepared by introducing the g-C3N4 dispersed Alg aqueous solution to a divalent Ca2+ ions solution.
32673713	1	76	theme	alginate	235:242	arg1	Alg					245:247	Alg	245:247	Alg	245:247	Herein, a simple method is used for fabrication of carbon nitride (g-C3N4) incorporated sodium alginate (Alg) beads.
32673713	1	76	theme	alginate	235:242	arg1	beads					250:254	sodium alginate (Alg) beads	228:254	sodium alginate (Alg) beads	228:254	Herein, a simple method is used for fabrication of carbon nitride (g-C3N4) incorporated sodium alginate (Alg) beads.
33677272	4	0	theme	humic-like	946:955	arg1	compounds					957:965	humic-like compounds	946:965	humic-like compounds	946:965	The organic carbon extracted was quantified and the nature of biochemical macromolecules (proteins, polysaccharides, and humic-like compounds) was evaluated using colorimetric methods.
33677272	9	1	theme	extraction	1706:1715	arg1	methods					1717:1723	'soft' extraction methods	1699:1723	'soft' extraction methods	1699:1723	Despite their low extraction efficiency, physical methods and CER, i.e. 'soft' extraction methods, are preferred using a solid:liquid ratio 1:40.
33677272	6	2	theme	reactant	1254:1261	arg1	salts					1263:1267	reactant salts	1254:1267	reactant salts	1254:1267	Moreover, chemical extraction presented a large quantity of impurities due to non-removal of reactant salts by ultracentrifugation.
33677272	2	3	theme	extraction	385:394	arg1	methods					396:402	proper extraction methods	378:402	proper extraction methods	378:402	Therefore, proper extraction methods are needed to study this EPS matrix.
33677272	7	4	theme	EPS	1391:1393	arg1	fraction					1365:1372	the main fraction	1356:1372	the main fraction of the extracted EPS	1356:1393	For the nine methods tested, humic-like substances represented the main fraction of the extracted EPS, but for chemical extraction, the presence of humic materials from the sediment organic fraction was due to non-specific extraction of the EPS fraction.
33677272	7	5	theme	sediment	1466:1473	arg1	fraction					1483:1490	the sediment organic fraction	1462:1490	the sediment organic fraction	1462:1490	For the nine methods tested, humic-like substances represented the main fraction of the extracted EPS, but for chemical extraction, the presence of humic materials from the sediment organic fraction was due to non-specific extraction of the EPS fraction.
33677272	2	6	theme	proper	378:383	arg1	methods					396:402	proper extraction methods	378:402	proper extraction methods	378:402	Therefore, proper extraction methods are needed to study this EPS matrix.
33677272	7	7	theme	EPS	1534:1536	arg1	fraction					1538:1545	the EPS fraction	1530:1545	the EPS fraction	1530:1545	For the nine methods tested, humic-like substances represented the main fraction of the extracted EPS, but for chemical extraction, the presence of humic materials from the sediment organic fraction was due to non-specific extraction of the EPS fraction.
33677272	7	8	theme	humic	1441:1445	arg1	materials					1447:1455	humic materials	1441:1455	humic materials from the sediment organic fraction	1441:1490	For the nine methods tested, humic-like substances represented the main fraction of the extracted EPS, but for chemical extraction, the presence of humic materials from the sediment organic fraction was due to non-specific extraction of the EPS fraction.
33677272	3	9	dep	physical	488:495	arg1	centrifugation					498:511	centrifugation	498:511	centrifugation	498:511	In this work, nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid:liquid ratio used for extraction, were compared based on the quantity and compositions of extracted EPS.
33677272	3	9	dep	physical	488:495	arg1	sonication					514:523	sonication	514:523	sonication	514:523	In this work, nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid:liquid ratio used for extraction, were compared based on the quantity and compositions of extracted EPS.
33677272	3	10	dep	chemical	527:534	arg1	hydroxide					544:552	sodium hydroxide	537:552	sodium hydroxide	537:552	In this work, nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid:liquid ratio used for extraction, were compared based on the quantity and compositions of extracted EPS.
33677272	3	10	dep	chemical	527:534	arg1	pyrophosphate					562:574	sodium pyrophosphate	555:574	sodium pyrophosphate	555:574	In this work, nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid:liquid ratio used for extraction, were compared based on the quantity and compositions of extracted EPS.
33677272	3	10	dep	chemical	527:534	arg1	tetraborate					584:594	sodium tetraborate	577:594	sodium tetraborate	577:594	In this work, nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid:liquid ratio used for extraction, were compared based on the quantity and compositions of extracted EPS.
33677272	0	11	theme	crystalline	92:102	arg1	bedrock					104:110	crystalline bedrock	92:110	crystalline bedrock	92:110	Extraction of extracellular polymeric substances from dam lake fresh sediments derived from crystalline bedrock.
33677272	4	12	theme	organic	829:835	arg1	carbon					837:842	The organic carbon	825:842	The organic carbon extracted	825:852	The organic carbon extracted was quantified and the nature of biochemical macromolecules (proteins, polysaccharides, and humic-like compounds) was evaluated using colorimetric methods.
33677272	7	13	theme	chemical	1404:1411	arg1	extraction					1413:1422	chemical extraction	1404:1422	chemical extraction	1404:1422	For the nine methods tested, humic-like substances represented the main fraction of the extracted EPS, but for chemical extraction, the presence of humic materials from the sediment organic fraction was due to non-specific extraction of the EPS fraction.
33677272	1	14	theme	sediment	280:287	arg1	particles					289:297	sediment particles	280:297	sediment particles	280:297	Extracellular polymeric substances (EPS) produced by microorganisms have a key role in the sedimentary compartment, e.g. promoting aggregation and biostabilisation of sediment particles and increasing chemical reactivity at the water/sediment interface.
33677272	8	15	from	sediment	1617:1624	arg1	EPS					1608:1610	extract EPS	1600:1610	extract EPS from sediment	1600:1624	Therefore, chemicals methods are not recommended to extract EPS from sediment.
33677272	9	16	theme	solid	1748:1752	arg1	1:40					1767:1770	a solid:liquid ratio 1:40	1746:1770	a solid:liquid ratio 1:40	1746:1770	Despite their low extraction efficiency, physical methods and CER, i.e. 'soft' extraction methods, are preferred using a solid:liquid ratio 1:40.
33677272	8	17	theme	extract	1600:1606	arg1	EPS					1608:1610	extract EPS	1600:1610	extract EPS from sediment	1600:1624	Therefore, chemicals methods are not recommended to extract EPS from sediment.
33677272	5	18	theme	cell	1056:1059	arg1	lysis					1061:1065	cell lysis	1056:1065	cell lysis	1056:1065	The amount of ATP was used as an indicator of cell lysis and showed contamination with intracellular materials in EPS extracted with chemical methods.
33677272	6	19	theme	impurities	1221:1230	arg1	quantity					1209:1216	a large quantity	1201:1216	a large quantity of impurities	1201:1230	Moreover, chemical extraction presented a large quantity of impurities due to non-removal of reactant salts by ultracentrifugation.
33677272	3	20	theme	sodium	537:542	arg1	hydroxide					544:552	sodium hydroxide	537:552	sodium hydroxide	537:552	In this work, nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid:liquid ratio used for extraction, were compared based on the quantity and compositions of extracted EPS.
33677272	3	20	theme	sodium	537:542	arg1	pyrophosphate					562:574	sodium pyrophosphate	555:574	sodium pyrophosphate	555:574	In this work, nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid:liquid ratio used for extraction, were compared based on the quantity and compositions of extracted EPS.
33677272	3	20	theme	sodium	537:542	arg1	tetraborate					584:594	sodium tetraborate	577:594	sodium tetraborate	577:594	In this work, nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid:liquid ratio used for extraction, were compared based on the quantity and compositions of extracted EPS.
33677272	4	21	theme	colorimetric	988:999	arg1	methods					1001:1007	colorimetric methods	988:1007	colorimetric methods	988:1007	The organic carbon extracted was quantified and the nature of biochemical macromolecules (proteins, polysaccharides, and humic-like compounds) was evaluated using colorimetric methods.
33677272	5	22	theme	lysis	1061:1065	arg1	indicator					1043:1051	an indicator	1040:1051	an indicator of cell lysis	1040:1065	The amount of ATP was used as an indicator of cell lysis and showed contamination with intracellular materials in EPS extracted with chemical methods.
33677272	5	22	theme	lysis	1061:1065	arg1	ATP					1024:1026	ATP	1024:1026	ATP	1024:1026	The amount of ATP was used as an indicator of cell lysis and showed contamination with intracellular materials in EPS extracted with chemical methods.
33677272	5	22	theme	lysis	1061:1065	arg1	contamination					1078:1090	contamination	1078:1090	contamination with intracellular materials	1078:1119	The amount of ATP was used as an indicator of cell lysis and showed contamination with intracellular materials in EPS extracted with chemical methods.
33677272	5	22	theme	lysis	1061:1065	arg1	amount					1014:1019	The amount	1010:1019	The amount of ATP	1010:1026	The amount of ATP was used as an indicator of cell lysis and showed contamination with intracellular materials in EPS extracted with chemical methods.
33677272	9	23	theme	extraction	1645:1654	arg1	efficiency					1656:1665	their low extraction efficiency	1635:1665	their low extraction efficiency	1635:1665	Despite their low extraction efficiency, physical methods and CER, i.e. 'soft' extraction methods, are preferred using a solid:liquid ratio 1:40.
33677272	7	24	theme	extracted	1381:1389	arg1	EPS					1391:1393	the extracted EPS	1377:1393	the extracted EPS	1377:1393	For the nine methods tested, humic-like substances represented the main fraction of the extracted EPS, but for chemical extraction, the presence of humic materials from the sediment organic fraction was due to non-specific extraction of the EPS fraction.
33677272	1	25	theme	key	188:190	arg1	role					192:195	a key role	186:195	a key role	186:195	Extracellular polymeric substances (EPS) produced by microorganisms have a key role in the sedimentary compartment, e.g. promoting aggregation and biostabilisation of sediment particles and increasing chemical reactivity at the water/sediment interface.
33677272	7	26	from	presence	1429:1436	arg1	fraction					1483:1490	the sediment organic fraction	1462:1490	the sediment organic fraction	1462:1490	For the nine methods tested, humic-like substances represented the main fraction of the extracted EPS, but for chemical extraction, the presence of humic materials from the sediment organic fraction was due to non-specific extraction of the EPS fraction.
33677272	1	27	theme	particles	289:297	arg1	biostabilisation					260:275	biostabilisation	260:275	biostabilisation	260:275	Extracellular polymeric substances (EPS) produced by microorganisms have a key role in the sedimentary compartment, e.g. promoting aggregation and biostabilisation of sediment particles and increasing chemical reactivity at the water/sediment interface.
33677272	1	27	theme	particles	289:297	arg1	aggregation					244:254	aggregation	244:254	aggregation	244:254	Extracellular polymeric substances (EPS) produced by microorganisms have a key role in the sedimentary compartment, e.g. promoting aggregation and biostabilisation of sediment particles and increasing chemical reactivity at the water/sediment interface.
33677272	5	28	theme	ATP	1024:1026	arg1	indicator					1043:1051	an indicator	1040:1051	an indicator of cell lysis	1040:1065	The amount of ATP was used as an indicator of cell lysis and showed contamination with intracellular materials in EPS extracted with chemical methods.
33677272	5	28	theme	ATP	1024:1026	arg1	ATP					1024:1026	ATP	1024:1026	ATP	1024:1026	The amount of ATP was used as an indicator of cell lysis and showed contamination with intracellular materials in EPS extracted with chemical methods.
33677272	5	28	theme	ATP	1024:1026	arg1	contamination					1078:1090	contamination	1078:1090	contamination with intracellular materials	1078:1119	The amount of ATP was used as an indicator of cell lysis and showed contamination with intracellular materials in EPS extracted with chemical methods.
33677272	5	28	theme	ATP	1024:1026	arg1	amount					1014:1019	The amount	1010:1019	The amount of ATP	1010:1026	The amount of ATP was used as an indicator of cell lysis and showed contamination with intracellular materials in EPS extracted with chemical methods.
33677272	3	29	theme	sodium	577:582	arg1	hydroxide					544:552	sodium hydroxide	537:552	sodium hydroxide	537:552	In this work, nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid:liquid ratio used for extraction, were compared based on the quantity and compositions of extracted EPS.
33677272	3	29	theme	sodium	577:582	arg1	tetraborate					584:594	sodium tetraborate	577:594	sodium tetraborate	577:594	In this work, nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid:liquid ratio used for extraction, were compared based on the quantity and compositions of extracted EPS.
33677272	7	30	theme	organic	1475:1481	arg1	fraction					1483:1490	the sediment organic fraction	1462:1490	the sediment organic fraction	1462:1490	For the nine methods tested, humic-like substances represented the main fraction of the extracted EPS, but for chemical extraction, the presence of humic materials from the sediment organic fraction was due to non-specific extraction of the EPS fraction.
33677272	7	31	theme	non-specific	1503:1514	arg1	extraction					1516:1525	non-specific extraction	1503:1525	non-specific extraction of the EPS fraction	1503:1545	For the nine methods tested, humic-like substances represented the main fraction of the extracted EPS, but for chemical extraction, the presence of humic materials from the sediment organic fraction was due to non-specific extraction of the EPS fraction.
33677272	0	32	theme	polymeric	28:36	arg1	substances					38:47	extracellular polymeric substances	14:47	extracellular polymeric substances	14:47	Extraction of extracellular polymeric substances from dam lake fresh sediments derived from crystalline bedrock.
33677272	3	33	theme	liquid	720:725	arg1	ratio					727:731	liquid ratio	720:731	liquid ratio used for extraction	720:751	In this work, nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid:liquid ratio used for extraction, were compared based on the quantity and compositions of extracted EPS.
33677272	3	34	theme	sodium	555:560	arg1	hydroxide					544:552	sodium hydroxide	537:552	sodium hydroxide	537:552	In this work, nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid:liquid ratio used for extraction, were compared based on the quantity and compositions of extracted EPS.
33677272	3	34	theme	sodium	555:560	arg1	pyrophosphate					562:574	sodium pyrophosphate	555:574	sodium pyrophosphate	555:574	In this work, nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid:liquid ratio used for extraction, were compared based on the quantity and compositions of extracted EPS.
33677272	7	35	theme	main	1360:1363	arg1	fraction					1365:1372	the main fraction	1356:1372	the main fraction of the extracted EPS	1356:1393	For the nine methods tested, humic-like substances represented the main fraction of the extracted EPS, but for chemical extraction, the presence of humic materials from the sediment organic fraction was due to non-specific extraction of the EPS fraction.
33677272	0	36	theme	extracellular	14:26	arg1	substances					38:47	extracellular polymeric substances	14:47	extracellular polymeric substances	14:47	Extraction of extracellular polymeric substances from dam lake fresh sediments derived from crystalline bedrock.
33677272	5	37	theme	chemical	1143:1150	arg1	methods					1152:1158	chemical methods	1143:1158	chemical methods	1143:1158	The amount of ATP was used as an indicator of cell lysis and showed contamination with intracellular materials in EPS extracted with chemical methods.
33677272	9	38	theme	physical	1668:1675	arg1	methods					1677:1683	physical methods	1668:1683	physical methods	1668:1683	Despite their low extraction efficiency, physical methods and CER, i.e. 'soft' extraction methods, are preferred using a solid:liquid ratio 1:40.
33677272	3	39	theme	exchange	637:644	arg1	resins					646:651	cation exchange resins	630:651	cation exchange resins	630:651	In this work, nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid:liquid ratio used for extraction, were compared based on the quantity and compositions of extracted EPS.
33677272	3	39	theme	exchange	637:644	arg1	CER					659:661	i.e. CER	654:661	i.e. CER	654:661	In this work, nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid:liquid ratio used for extraction, were compared based on the quantity and compositions of extracted EPS.
33677272	1	40	theme	Extracellular	113:125	arg1	EPS					149:151	EPS	149:151	EPS	149:151	Extracellular polymeric substances (EPS) produced by microorganisms have a key role in the sedimentary compartment, e.g. promoting aggregation and biostabilisation of sediment particles and increasing chemical reactivity at the water/sediment interface.
33677272	1	40	theme	Extracellular	113:125	arg1	substances					137:146	Extracellular polymeric substances	113:146	Extracellular polymeric substances (EPS) produced by microorganisms	113:179	Extracellular polymeric substances (EPS) produced by microorganisms have a key role in the sedimentary compartment, e.g. promoting aggregation and biostabilisation of sediment particles and increasing chemical reactivity at the water/sediment interface.
33677272	3	41	dep	chemical	607:614	arg1	resins					646:651	cation exchange resins	630:651	cation exchange resins	630:651	In this work, nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid:liquid ratio used for extraction, were compared based on the quantity and compositions of extracted EPS.
33677272	3	41	dep	chemical	607:614	arg1	CER					659:661	i.e. CER	654:661	i.e. CER	654:661	In this work, nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid:liquid ratio used for extraction, were compared based on the quantity and compositions of extracted EPS.
33677272	9	42	theme	ratio	1761:1765	arg1	1:40					1767:1770	a solid:liquid ratio 1:40	1746:1770	a solid:liquid ratio 1:40	1746:1770	Despite their low extraction efficiency, physical methods and CER, i.e. 'soft' extraction methods, are preferred using a solid:liquid ratio 1:40.
33677272	1	43	theme	chemical	314:321	arg1	reactivity					323:332	chemical reactivity	314:332	chemical reactivity	314:332	Extracellular polymeric substances (EPS) produced by microorganisms have a key role in the sedimentary compartment, e.g. promoting aggregation and biostabilisation of sediment particles and increasing chemical reactivity at the water/sediment interface.
33677272	4	44	theme	biochemical	887:897	arg1	macromolecules					899:912	biochemical macromolecules	887:912	biochemical macromolecules (proteins, polysaccharides, and humic-like compounds)	887:966	The organic carbon extracted was quantified and the nature of biochemical macromolecules (proteins, polysaccharides, and humic-like compounds) was evaluated using colorimetric methods.
33677272	1	45	theme	sedimentary	204:214	arg1	compartment					216:226	the sedimentary compartment	200:226	the sedimentary compartment	200:226	Extracellular polymeric substances (EPS) produced by microorganisms have a key role in the sedimentary compartment, e.g. promoting aggregation and biostabilisation of sediment particles and increasing chemical reactivity at the water/sediment interface.
33677272	6	46	theme	large	1203:1207	arg1	quantity					1209:1216	a large quantity	1201:1216	a large quantity of impurities	1201:1230	Moreover, chemical extraction presented a large quantity of impurities due to non-removal of reactant salts by ultracentrifugation.
33677272	3	47	theme	extraction	460:469	arg1	methods					471:477	nine extraction methods	455:477	nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid	455:718	In this work, nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid:liquid ratio used for extraction, were compared based on the quantity and compositions of extracted EPS.
33677272	0	48	theme	substances	38:47	arg1	Extraction					0:9	Extraction	0:9	Extraction of extracellular polymeric substances from dam lake fresh sediments	0:77	Extraction of extracellular polymeric substances from dam lake fresh sediments derived from crystalline bedrock.
33677272	5	49	theme	intracellular	1097:1109	arg1	materials					1111:1119	intracellular materials	1097:1119	intracellular materials	1097:1119	The amount of ATP was used as an indicator of cell lysis and showed contamination with intracellular materials in EPS extracted with chemical methods.
33677272	4	50	dep	macromolecules	899:912	arg1	proteins					915:922	proteins	915:922	proteins	915:922	The organic carbon extracted was quantified and the nature of biochemical macromolecules (proteins, polysaccharides, and humic-like compounds) was evaluated using colorimetric methods.
33677272	4	50	dep	macromolecules	899:912	arg1	compounds					957:965	humic-like compounds	946:965	humic-like compounds	946:965	The organic carbon extracted was quantified and the nature of biochemical macromolecules (proteins, polysaccharides, and humic-like compounds) was evaluated using colorimetric methods.
33677272	4	50	dep	macromolecules	899:912	arg1	polysaccharides					925:939	polysaccharides	925:939	polysaccharides	925:939	The organic carbon extracted was quantified and the nature of biochemical macromolecules (proteins, polysaccharides, and humic-like compounds) was evaluated using colorimetric methods.
33677272	0	51	from	sediments	69:77	arg1	Extraction					0:9	Extraction	0:9	Extraction of extracellular polymeric substances from dam lake fresh sediments	0:77	Extraction of extracellular polymeric substances from dam lake fresh sediments derived from crystalline bedrock.
33677272	2	52	theme	EPS	429:431	arg1	matrix					433:438	this EPS matrix	424:438	this EPS matrix	424:438	Therefore, proper extraction methods are needed to study this EPS matrix.
33677272	0	53	theme	lake	58:61	arg1	sediments					69:77	dam lake fresh sediments	54:77	dam lake fresh sediments	54:77	Extraction of extracellular polymeric substances from dam lake fresh sediments derived from crystalline bedrock.
33677272	9	54	dep	methods	1717:1723	arg1	i.e.					1694:1697	i.e.	1694:1697	i.e.	1694:1697	Despite their low extraction efficiency, physical methods and CER, i.e. 'soft' extraction methods, are preferred using a solid:liquid ratio 1:40.
33677272	1	55	contain	have	181:184	arg1	EPS					149:151	EPS	149:151	EPS	149:151	Extracellular polymeric substances (EPS) produced by microorganisms have a key role in the sedimentary compartment, e.g. promoting aggregation and biostabilisation of sediment particles and increasing chemical reactivity at the water/sediment interface.
33677272	1	55	contain	have	181:184	arg2	role					192:195	a key role	186:195	a key role	186:195	Extracellular polymeric substances (EPS) produced by microorganisms have a key role in the sedimentary compartment, e.g. promoting aggregation and biostabilisation of sediment particles and increasing chemical reactivity at the water/sediment interface.
33677272	1	55	contain	have	181:184	arg1	substances					137:146	Extracellular polymeric substances	113:146	Extracellular polymeric substances (EPS) produced by microorganisms	113:179	Extracellular polymeric substances (EPS) produced by microorganisms have a key role in the sedimentary compartment, e.g. promoting aggregation and biostabilisation of sediment particles and increasing chemical reactivity at the water/sediment interface.
33677272	1	56	dep	have	181:184	arg1	promoting					234:242	promoting	234:242	promoting aggregation and biostabilisation of sediment particles	234:297	Extracellular polymeric substances (EPS) produced by microorganisms have a key role in the sedimentary compartment, e.g. promoting aggregation and biostabilisation of sediment particles and increasing chemical reactivity at the water/sediment interface.
33677272	1	56	dep	have	181:184	arg1	increasing					303:312	increasing	303:312	increasing chemical reactivity at the water/sediment interface	303:364	Extracellular polymeric substances (EPS) produced by microorganisms have a key role in the sedimentary compartment, e.g. promoting aggregation and biostabilisation of sediment particles and increasing chemical reactivity at the water/sediment interface.
33677272	1	57	theme	polymeric	127:135	arg1	EPS					149:151	EPS	149:151	EPS	149:151	Extracellular polymeric substances (EPS) produced by microorganisms have a key role in the sedimentary compartment, e.g. promoting aggregation and biostabilisation of sediment particles and increasing chemical reactivity at the water/sediment interface.
33677272	1	57	theme	polymeric	127:135	arg1	substances					137:146	Extracellular polymeric substances	113:146	Extracellular polymeric substances (EPS) produced by microorganisms	113:179	Extracellular polymeric substances (EPS) produced by microorganisms have a key role in the sedimentary compartment, e.g. promoting aggregation and biostabilisation of sediment particles and increasing chemical reactivity at the water/sediment interface.
33677272	0	58	theme	dam	54:56	arg1	sediments					69:77	dam lake fresh sediments	54:77	dam lake fresh sediments	54:77	Extraction of extracellular polymeric substances from dam lake fresh sediments derived from crystalline bedrock.
33677272	5	59	used	used	1032:1035	arg2	indicator					1043:1051	an indicator	1040:1051	an indicator of cell lysis	1040:1065	The amount of ATP was used as an indicator of cell lysis and showed contamination with intracellular materials in EPS extracted with chemical methods.
33677272	5	59	used	used	1032:1035	arg2	contamination					1078:1090	contamination	1078:1090	contamination with intracellular materials	1078:1119	The amount of ATP was used as an indicator of cell lysis and showed contamination with intracellular materials in EPS extracted with chemical methods.
33677272	5	59	used	used	1032:1035	arg2	ATP					1024:1026	ATP	1024:1026	ATP	1024:1026	The amount of ATP was used as an indicator of cell lysis and showed contamination with intracellular materials in EPS extracted with chemical methods.
33677272	5	59	used	used	1032:1035	arg2	amount					1014:1019	The amount	1010:1019	The amount of ATP	1010:1026	The amount of ATP was used as an indicator of cell lysis and showed contamination with intracellular materials in EPS extracted with chemical methods.
33677272	3	60	theme	cation	630:635	arg1	resins					646:651	cation exchange resins	630:651	cation exchange resins	630:651	In this work, nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid:liquid ratio used for extraction, were compared based on the quantity and compositions of extracted EPS.
33677272	3	60	theme	cation	630:635	arg1	CER					659:661	i.e. CER	654:661	i.e. CER	654:661	In this work, nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid:liquid ratio used for extraction, were compared based on the quantity and compositions of extracted EPS.
33677272	9	61	theme	low	1641:1643	arg1	efficiency					1656:1665	their low extraction efficiency	1635:1665	their low extraction efficiency	1635:1665	Despite their low extraction efficiency, physical methods and CER, i.e. 'soft' extraction methods, are preferred using a solid:liquid ratio 1:40.
33677272	7	62	from	fraction	1483:1490	arg1	due					1496:1498	due	1496:1498	due	1496:1498	For the nine methods tested, humic-like substances represented the main fraction of the extracted EPS, but for chemical extraction, the presence of humic materials from the sediment organic fraction was due to non-specific extraction of the EPS fraction.
33677272	7	62	from	fraction	1483:1490	arg1	presence					1429:1436	the presence	1425:1436	the presence of humic materials from the sediment organic fraction	1425:1490	For the nine methods tested, humic-like substances represented the main fraction of the extracted EPS, but for chemical extraction, the presence of humic materials from the sediment organic fraction was due to non-specific extraction of the EPS fraction.
33677272	7	62	from	fraction	1483:1490	arg1	materials					1447:1455	humic materials	1441:1455	humic materials from the sediment organic fraction	1441:1490	For the nine methods tested, humic-like substances represented the main fraction of the extracted EPS, but for chemical extraction, the presence of humic materials from the sediment organic fraction was due to non-specific extraction of the EPS fraction.
33677272	3	63	theme	extracted	810:818	arg1	EPS					820:822	extracted EPS	810:822	extracted EPS	810:822	In this work, nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid:liquid ratio used for extraction, were compared based on the quantity and compositions of extracted EPS.
33677272	1	64	theme	water/sediment	341:354	arg1	interface					356:364	the water/sediment interface	337:364	the water/sediment interface	337:364	Extracellular polymeric substances (EPS) produced by microorganisms have a key role in the sedimentary compartment, e.g. promoting aggregation and biostabilisation of sediment particles and increasing chemical reactivity at the water/sediment interface.
33677272	7	65	theme	humic-like	1322:1331	arg1	substances					1333:1342	humic-like substances	1322:1342	humic-like substances	1322:1342	For the nine methods tested, humic-like substances represented the main fraction of the extracted EPS, but for chemical extraction, the presence of humic materials from the sediment organic fraction was due to non-specific extraction of the EPS fraction.
33677272	6	66	theme	chemical	1171:1178	arg1	extraction					1180:1189	chemical extraction	1171:1189	chemical extraction	1171:1189	Moreover, chemical extraction presented a large quantity of impurities due to non-removal of reactant salts by ultracentrifugation.
33677272	3	67	theme	physical	488:495	arg1	treatments					664:673	physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments	488:673	physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments	488:673	In this work, nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid:liquid ratio used for extraction, were compared based on the quantity and compositions of extracted EPS.
33677272	3	68	theme	EPS	820:822	arg1	compositions					794:805	compositions	794:805	compositions	794:805	In this work, nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid:liquid ratio used for extraction, were compared based on the quantity and compositions of extracted EPS.
33677272	3	68	theme	EPS	820:822	arg1	quantity					781:788	the quantity	777:788	the quantity	777:788	In this work, nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid:liquid ratio used for extraction, were compared based on the quantity and compositions of extracted EPS.
33677272	6	69	theme	salts	1263:1267	arg1	non-removal					1239:1249	non-removal	1239:1249	non-removal of reactant salts by ultracentrifugation	1239:1290	Moreover, chemical extraction presented a large quantity of impurities due to non-removal of reactant salts by ultracentrifugation.
33677272	3	70	dep	methods	471:477	arg1	ratio					727:731	liquid ratio	720:731	liquid ratio used for extraction	720:751	In this work, nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid:liquid ratio used for extraction, were compared based on the quantity and compositions of extracted EPS.
33677272	8	71	theme	chemicals	1559:1567	arg1	methods					1569:1575	chemicals methods	1559:1575	chemicals methods	1559:1575	Therefore, chemicals methods are not recommended to extract EPS from sediment.
33677272	9	72	theme	soft	1700:1703	arg1	methods					1717:1723	'soft' extraction methods	1699:1723	'soft' extraction methods	1699:1723	Despite their low extraction efficiency, physical methods and CER, i.e. 'soft' extraction methods, are preferred using a solid:liquid ratio 1:40.
33677272	3	73	theme	i.e.	654:657	arg1	resins					646:651	cation exchange resins	630:651	cation exchange resins	630:651	In this work, nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid:liquid ratio used for extraction, were compared based on the quantity and compositions of extracted EPS.
33677272	3	73	theme	i.e.	654:657	arg1	CER					659:661	i.e. CER	654:661	i.e. CER	654:661	In this work, nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid:liquid ratio used for extraction, were compared based on the quantity and compositions of extracted EPS.
33677272	9	74	dep	solid	1748:1752	arg1	liquid					1754:1759	liquid	1754:1759	liquid	1754:1759	Despite their low extraction efficiency, physical methods and CER, i.e. 'soft' extraction methods, are preferred using a solid:liquid ratio 1:40.
33677272	7	75	theme	fraction	1538:1545	arg1	extraction					1516:1525	non-specific extraction	1503:1525	non-specific extraction of the EPS fraction	1503:1545	For the nine methods tested, humic-like substances represented the main fraction of the extracted EPS, but for chemical extraction, the presence of humic materials from the sediment organic fraction was due to non-specific extraction of the EPS fraction.
33677272	0	76	theme	fresh	63:67	arg1	sediments					69:77	dam lake fresh sediments	54:77	dam lake fresh sediments	54:77	Extraction of extracellular polymeric substances from dam lake fresh sediments derived from crystalline bedrock.
33677272	7	77	theme	materials	1447:1455	arg1	due					1496:1498	due	1496:1498	due	1496:1498	For the nine methods tested, humic-like substances represented the main fraction of the extracted EPS, but for chemical extraction, the presence of humic materials from the sediment organic fraction was due to non-specific extraction of the EPS fraction.
33677272	7	77	theme	materials	1447:1455	arg1	presence					1429:1436	the presence	1425:1436	the presence of humic materials from the sediment organic fraction	1425:1490	For the nine methods tested, humic-like substances represented the main fraction of the extracted EPS, but for chemical extraction, the presence of humic materials from the sediment organic fraction was due to non-specific extraction of the EPS fraction.
33677272	3	78	theme	chemical	607:614	arg1	treatments					664:673	physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments	488:673	physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments	488:673	In this work, nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid:liquid ratio used for extraction, were compared based on the quantity and compositions of extracted EPS.
33677272	3	79	theme	chemical	527:534	arg1	treatments					664:673	physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments	488:673	physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments	488:673	In this work, nine extraction methods based on physical (centrifugation, sonication), chemical (sodium hydroxide, sodium pyrophosphate, sodium tetraborate), and both chemical and physical (cation exchange resins, i.e. CER) treatments and their combinations, as well as the solid:liquid ratio used for extraction, were compared based on the quantity and compositions of extracted EPS.
33677272	5	80	with	contamination	1078:1090	arg1	materials					1111:1119	intracellular materials	1097:1119	intracellular materials	1097:1119	The amount of ATP was used as an indicator of cell lysis and showed contamination with intracellular materials in EPS extracted with chemical methods.
33677272	4	81	theme	macromolecules	899:912	arg1	nature					877:882	the nature	873:882	the nature of biochemical macromolecules (proteins, polysaccharides, and humic-like compounds)	873:966	The organic carbon extracted was quantified and the nature of biochemical macromolecules (proteins, polysaccharides, and humic-like compounds) was evaluated using colorimetric methods.
32298317	15	0	theme	great	2809:2813	arg1	potential					2815:2823	a great potential	2807:2823	a great potential to be used as health-promoting agents in the farming of European sea bass and other marine fish	2807:2919	Therefore, these functional ingredients have a great potential to be used as health-promoting agents in the farming of European sea bass and other marine fish.
32298317	9	1	theme	major	1714:1718	arg1	feedstuffs					1697:1706	feedstuffs	1697:1706	feedstuffs	1697:1706	The variation of resident gut microbiota induced by diet was lower than the variation of transient intestinal microbiota, because feedstuffs are a major source of allochthonous bacteria, which can temporarily integrate into the gut transient microbiome.
32298317	9	1	theme	major	1714:1718	arg1	source					1720:1725	a major source	1712:1725	a major source	1712:1725	The variation of resident gut microbiota induced by diet was lower than the variation of transient intestinal microbiota, because feedstuffs are a major source of allochthonous bacteria, which can temporarily integrate into the gut transient microbiome.
32298317	0	2	theme	fish	173:176	arg1	oil					178:180	fish oil	173:180	fish oil	173:180	Assessment of dietary supplementation with galactomannan oligosaccharides and phytogenics on gut microbiota of European sea bass (Dicentrarchus Labrax) fed low fishmeal and fish oil based diet.
32298317	12	3	theme	FO	2117:2118	arg1	diet					2120:2123	FO diet	2117:2123	FO diet	2117:2123	Our findings suggest that the dietary inclusion of GMOS (0.5%) and PHYTO (0.02%) in a low FM and FO diet induces changes in gut microbiota composition of European sea bass.
32298317	12	4	theme	gut	2144:2146	arg1	composition					2159:2169	gut microbiota composition	2144:2169	gut microbiota composition of European sea bass	2144:2190	Our findings suggest that the dietary inclusion of GMOS (0.5%) and PHYTO (0.02%) in a low FM and FO diet induces changes in gut microbiota composition of European sea bass.
32298317	3	5	from	mixture	626:632	arg1	rich					577:580	rich	577:580	rich	577:580	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	0	6	theme	based	182:186	arg1	diet					188:191	low fishmeal and fish oil based diet	156:191	low fishmeal and fish oil based diet	156:191	Assessment of dietary supplementation with galactomannan oligosaccharides and phytogenics on gut microbiota of European sea bass (Dicentrarchus Labrax) fed low fishmeal and fish oil based diet.
32298317	3	7	theme	inclusion	545:553	arg1	effects					526:532	the effects	522:532	the effects	522:532	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	13	8	theme	resident	2473:2480	arg1	orders					2492:2497	resident bacterial orders	2473:2497	resident bacterial orders	2473:2497	However, if on allochthonous microbiota the combined inclusion of GMOS and PHYTO showed an antagonistic effect on bactericidal activity against Vibrionales, at mucosa level, only GMOSPHYTO diet increased the relative abundance of Bacteroidales, Lactobacillales, and Clostridiales resident bacterial orders.
32298317	9	9	theme	bacteria	1744:1751	arg1	feedstuffs					1697:1706	feedstuffs	1697:1706	feedstuffs	1697:1706	The variation of resident gut microbiota induced by diet was lower than the variation of transient intestinal microbiota, because feedstuffs are a major source of allochthonous bacteria, which can temporarily integrate into the gut transient microbiome.
32298317	9	9	theme	bacteria	1744:1751	arg1	source					1720:1725	a major source	1712:1725	a major source	1712:1725	The variation of resident gut microbiota induced by diet was lower than the variation of transient intestinal microbiota, because feedstuffs are a major source of allochthonous bacteria, which can temporarily integrate into the gut transient microbiome.
32298317	14	10	theme	microbiota	2710:2719	arg1	composition					2721:2731	gut microbiota composition	2706:2731	gut microbiota composition	2706:2731	The main beneficial effects of GMOS and PHYTO on gut microbiota are the reduction of coliforms and Vibrionales bacteria, which include several potentially pathogenic species for fish, and the enrichment of gut microbiota composition with butyrate producer taxa.
32298317	11	11	theme	mucosa	2003:2008	arg1	profiles					2010:2017	mucosa profiles	2003:2017	mucosa profiles	2003:2017	Indeed, the microbial profile of feeds was different from both faecal and mucosa profiles.
32298317	11	12	theme	feeds	1962:1966	arg1	profile					1951:1957	the microbial profile	1937:1957	the microbial profile of feeds	1937:1966	Indeed, the microbial profile of feeds was different from both faecal and mucosa profiles.
32298317	11	12	theme	feeds	1962:1966	arg1	different					1972:1980	different	1972:1980	different	1972:1980	Indeed, the microbial profile of feeds was different from both faecal and mucosa profiles.
32298317	10	13	dep	a	1896:1896	arg1	mirror					1898:1903	mirror	1898:1903	mirror	1898:1903	However, the composition of transient bacterial communities was not simply a mirror of feed-borne bacteria.
32298317	3	14	from	combination	684:694	arg1	rich					577:580	rich	577:580	rich	577:580	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	3	15	theme	extract	569:575	arg1	inclusion					545:553	dietary inclusion	537:553	dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO)	537:714	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	13	16	theme	Lactobacillales	2438:2452	arg1	abundance					2410:2418	the relative abundance	2397:2418	the relative abundance of Bacteroidales, Lactobacillales, and Clostridiales resident bacterial orders	2397:2497	However, if on allochthonous microbiota the combined inclusion of GMOS and PHYTO showed an antagonistic effect on bactericidal activity against Vibrionales, at mucosa level, only GMOSPHYTO diet increased the relative abundance of Bacteroidales, Lactobacillales, and Clostridiales resident bacterial orders.
32298317	13	17	theme	bactericidal	2307:2318	arg1	activity					2320:2327	bactericidal activity	2307:2327	bactericidal activity against Vibrionales	2307:2347	However, if on allochthonous microbiota the combined inclusion of GMOS and PHYTO showed an antagonistic effect on bactericidal activity against Vibrionales, at mucosa level, only GMOSPHYTO diet increased the relative abundance of Bacteroidales, Lactobacillales, and Clostridiales resident bacterial orders.
32298317	13	18	from	effect	2297:2302	arg1	activity					2320:2327	bactericidal activity	2307:2327	bactericidal activity against Vibrionales	2307:2347	However, if on allochthonous microbiota the combined inclusion of GMOS and PHYTO showed an antagonistic effect on bactericidal activity against Vibrionales, at mucosa level, only GMOSPHYTO diet increased the relative abundance of Bacteroidales, Lactobacillales, and Clostridiales resident bacterial orders.
32298317	13	19	dep	Bacteroidales	2423:2435	arg1	orders					2492:2497	resident bacterial orders	2473:2497	resident bacterial orders	2473:2497	However, if on allochthonous microbiota the combined inclusion of GMOS and PHYTO showed an antagonistic effect on bactericidal activity against Vibrionales, at mucosa level, only GMOSPHYTO diet increased the relative abundance of Bacteroidales, Lactobacillales, and Clostridiales resident bacterial orders.
32298317	15	20	from	agents	2856:2861	arg1	farming					2870:2876	the farming	2866:2876	the farming of European sea bass and other marine fish	2866:2919	Therefore, these functional ingredients have a great potential to be used as health-promoting agents in the farming of European sea bass and other marine fish.
32298317	14	21	theme	Vibrionales	2599:2609	arg1	bacteria					2611:2618	Vibrionales bacteria	2599:2618	Vibrionales bacteria	2599:2618	The main beneficial effects of GMOS and PHYTO on gut microbiota are the reduction of coliforms and Vibrionales bacteria, which include several potentially pathogenic species for fish, and the enrichment of gut microbiota composition with butyrate producer taxa.
32298317	4	22	theme	feeding	961:967	arg1	trial					969:973	a 63-days feeding trial	951:973	a 63-days feeding trial	951:973	Three experimental diets and a control diet (plant-based formulation with 10% FM and 6% FO) were tested in a 63-days feeding trial.
32298317	13	23	theme	GMOS	2259:2262	arg1	inclusion					2246:2254	the combined inclusion	2233:2254	the combined inclusion of GMOS and PHYTO	2233:2272	However, if on allochthonous microbiota the combined inclusion of GMOS and PHYTO showed an antagonistic effect on bactericidal activity against Vibrionales, at mucosa level, only GMOSPHYTO diet increased the relative abundance of Bacteroidales, Lactobacillales, and Clostridiales resident bacterial orders.
32298317	5	24	theme	16S	1171:1173	arg1	gene					1180:1183	16S rRNA gene	1171:1183	16S rRNA gene	1171:1183	To analyze the microbiota associated to feeds and the intestinal autochthonous (mucosa-adhered) and allochthonous (transient) microbial communities, the Illumina MiSeq platform for sequencing of 16S rRNA gene and QIIME2 pipeline were used.
32298317	15	25	theme	sea	2890:2892	arg1	bass					2894:2897	European sea bass	2881:2897	European sea bass	2881:2897	Therefore, these functional ingredients have a great potential to be used as health-promoting agents in the farming of European sea bass and other marine fish.
32298317	14	26	theme	coliforms	2585:2593	arg1	reduction					2572:2580	the reduction	2568:2580	the reduction	2568:2580	The main beneficial effects of GMOS and PHYTO on gut microbiota are the reduction of coliforms and Vibrionales bacteria, which include several potentially pathogenic species for fish, and the enrichment of gut microbiota composition with butyrate producer taxa.
32298317	14	26	theme	coliforms	2585:2593	arg1	effects					2520:2526	The main beneficial effects	2500:2526	The main beneficial effects of GMOS and PHYTO on gut microbiota	2500:2562	The main beneficial effects of GMOS and PHYTO on gut microbiota are the reduction of coliforms and Vibrionales bacteria, which include several potentially pathogenic species for fish, and the enrichment of gut microbiota composition with butyrate producer taxa.
32298317	3	27	theme	microbiota	724:733	arg1	composition					735:745	gut microbiota composition	720:745	gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet	720:841	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	1	28	from	industry	244:251	arg1	interest					217:224	an increasing interest	203:224	an increasing interest from the aquafeed industry in functional feeds containing selected additives that improve fish growth performance and health status	203:356	There is an increasing interest from the aquafeed industry in functional feeds containing selected additives that improve fish growth performance and health status.
32298317	0	29	from	phytogenics	78:88	arg1	microbiota					97:106	gut microbiota	93:106	gut microbiota of European sea bass (Dicentrarchus Labrax)	93:150	Assessment of dietary supplementation with galactomannan oligosaccharides and phytogenics on gut microbiota of European sea bass (Dicentrarchus Labrax) fed low fishmeal and fish oil based diet.
32298317	9	30	theme	gut	1795:1797	arg1	microbiome					1809:1818	the gut transient microbiome	1791:1818	the gut transient microbiome	1791:1818	The variation of resident gut microbiota induced by diet was lower than the variation of transient intestinal microbiota, because feedstuffs are a major source of allochthonous bacteria, which can temporarily integrate into the gut transient microbiome.
32298317	4	31	dep	diet	883:886	arg1	%					930:930	6% FO	929:933	6% FO	929:933	Three experimental diets and a control diet (plant-based formulation with 10% FM and 6% FO) were tested in a 63-days feeding trial.
32298317	4	31	dep	diet	883:886	arg1	formulation					901:911	plant-based formulation	889:911	plant-based formulation with 10% FM	889:923	Three experimental diets and a control diet (plant-based formulation with 10% FM and 6% FO) were tested in a 63-days feeding trial.
32298317	1	32	dep	functional	256:265	arg1	feeds					267:271	feeds	267:271	feeds	267:271	There is an increasing interest from the aquafeed industry in functional feeds containing selected additives that improve fish growth performance and health status.
32298317	8	33	dep	the	1528:1530	arg1	feeds					1532:1536	feeds	1532:1536	feeds	1532:1536	The OTU (operational taxonomic unit) number was instead similar between the feeds, ranging from 42 to 50 OTUs.
32298317	3	34	theme	sea	759:761	arg1	bass					763:766	European sea bass	750:766	European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet	750:841	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	3	34	theme	sea	759:761	arg1	labrax					783:788	Dicentrarchus labrax	769:788	Dicentrarchus labrax	769:788	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	1	35	from	interest	217:224	arg1	functional					256:265	functional	256:265	functional	256:265	There is an increasing interest from the aquafeed industry in functional feeds containing selected additives that improve fish growth performance and health status.
32298317	14	36	theme	pathogenic	2655:2664	arg1	species					2666:2672	several potentially pathogenic species	2635:2672	several potentially pathogenic species for fish	2635:2681	The main beneficial effects of GMOS and PHYTO on gut microbiota are the reduction of coliforms and Vibrionales bacteria, which include several potentially pathogenic species for fish, and the enrichment of gut microbiota composition with butyrate producer taxa.
32298317	4	37	theme	control	875:881	arg1	diet					883:886	a control diet	873:886	a control diet (plant-based formulation with 10% FM and 6% FO)	873:934	Three experimental diets and a control diet (plant-based formulation with 10% FM and 6% FO) were tested in a 63-days feeding trial.
32298317	14	38	theme	gut	2549:2551	arg1	microbiota					2553:2562	gut microbiota	2549:2562	gut microbiota	2549:2562	The main beneficial effects of GMOS and PHYTO on gut microbiota are the reduction of coliforms and Vibrionales bacteria, which include several potentially pathogenic species for fish, and the enrichment of gut microbiota composition with butyrate producer taxa.
32298317	6	39	theme	control	1308:1314	arg1	communities					1293:1303	the microbial communities	1279:1303	the microbial communities of control (CTRL)	1279:1321	Metabarcoding analysis of feed-associated bacteria showed that the microbial communities of control (CTRL) feed deeply differed from those of experimental diets.
32298317	10	40	theme	bacterial	1859:1867	arg1	communities					1869:1879	transient bacterial communities	1849:1879	transient bacterial communities	1849:1879	However, the composition of transient bacterial communities was not simply a mirror of feed-borne bacteria.
32298317	9	41	theme	gut	1593:1595	arg1	microbiota					1597:1606	resident gut microbiota	1584:1606	resident gut microbiota induced by diet	1584:1622	The variation of resident gut microbiota induced by diet was lower than the variation of transient intestinal microbiota, because feedstuffs are a major source of allochthonous bacteria, which can temporarily integrate into the gut transient microbiome.
32298317	12	42	theme	PHYTO	2087:2091	arg1	inclusion					2058:2066	the dietary inclusion	2046:2066	the dietary inclusion of GMOS (0.5%) and PHYTO (0.02%) in a low FM and FO diet	2046:2123	Our findings suggest that the dietary inclusion of GMOS (0.5%) and PHYTO (0.02%) in a low FM and FO diet induces changes in gut microbiota composition of European sea bass.
32298317	0	43	theme	fishmeal	160:167	arg1	diet					188:191	low fishmeal and fish oil based diet	156:191	low fishmeal and fish oil based diet	156:191	Assessment of dietary supplementation with galactomannan oligosaccharides and phytogenics on gut microbiota of European sea bass (Dicentrarchus Labrax) fed low fishmeal and fish oil based diet.
32298317	0	44	theme	gut	93:95	arg1	microbiota					97:106	gut microbiota	93:106	gut microbiota of European sea bass (Dicentrarchus Labrax)	93:150	Assessment of dietary supplementation with galactomannan oligosaccharides and phytogenics on gut microbiota of European sea bass (Dicentrarchus Labrax) fed low fishmeal and fish oil based diet.
32298317	1	45	theme	health	344:349	arg1	status					351:356	health status	344:356	health status	344:356	There is an increasing interest from the aquafeed industry in functional feeds containing selected additives that improve fish growth performance and health status.
32298317	4	46	theme	experimental	850:861	arg1	diets					863:867	Three experimental diets	844:867	Three experimental diets	844:867	Three experimental diets and a control diet (plant-based formulation with 10% FM and 6% FO) were tested in a 63-days feeding trial.
32298317	5	47	theme	MiSeq	1138:1142	arg1	platform					1144:1151	the Illumina MiSeq platform	1125:1151	the Illumina MiSeq platform for sequencing of 16S rRNA gene and QIIME2 pipeline	1125:1203	To analyze the microbiota associated to feeds and the intestinal autochthonous (mucosa-adhered) and allochthonous (transient) microbial communities, the Illumina MiSeq platform for sequencing of 16S rRNA gene and QIIME2 pipeline were used.
32298317	14	48	theme	producer	2747:2754	arg1	taxa					2756:2759	butyrate producer taxa	2738:2759	butyrate producer taxa	2738:2759	The main beneficial effects of GMOS and PHYTO on gut microbiota are the reduction of coliforms and Vibrionales bacteria, which include several potentially pathogenic species for fish, and the enrichment of gut microbiota composition with butyrate producer taxa.
32298317	2	49	attach	derived	460:466	arg1	plants					473:478	plants	473:478	plants	473:478	Functional feed additives include probiotics, prebiotics, organic acids, and phytogenics (substances derived from plants and their extracts).
32298317	2	49	attach	derived	460:466	arg2	substances					449:458	substances	449:458	substances derived from plants and their extracts	449:497	Functional feed additives include probiotics, prebiotics, organic acids, and phytogenics (substances derived from plants and their extracts).
32298317	2	49	attach	derived	460:466	arg1	extracts					490:497	their extracts	484:497	their extracts	484:497	Functional feed additives include probiotics, prebiotics, organic acids, and phytogenics (substances derived from plants and their extracts).
32298317	14	50	from	effects	2520:2526	arg1	microbiota					2553:2562	gut microbiota	2549:2562	gut microbiota	2549:2562	The main beneficial effects of GMOS and PHYTO on gut microbiota are the reduction of coliforms and Vibrionales bacteria, which include several potentially pathogenic species for fish, and the enrichment of gut microbiota composition with butyrate producer taxa.
32298317	3	51	theme	oil	829:831	arg1	diet					838:841	fish oil (FO) diet	824:841	fish oil (FO) diet	824:841	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	7	52	theme	CTRL	1425:1428	arg1	feed					1430:1433	CTRL feed	1425:1433	CTRL feed	1425:1433	The number of reads was significantly lower in CTRL feed than in other feeds.
32298317	0	53	from	oligosaccharides	57:72	arg1	microbiota					97:106	gut microbiota	93:106	gut microbiota of European sea bass (Dicentrarchus Labrax)	93:150	Assessment of dietary supplementation with galactomannan oligosaccharides and phytogenics on gut microbiota of European sea bass (Dicentrarchus Labrax) fed low fishmeal and fish oil based diet.
32298317	5	54	theme	pipeline	1196:1203	arg1	sequencing					1157:1166	sequencing	1157:1166	sequencing of 16S rRNA gene and QIIME2 pipeline	1157:1203	To analyze the microbiota associated to feeds and the intestinal autochthonous (mucosa-adhered) and allochthonous (transient) microbial communities, the Illumina MiSeq platform for sequencing of 16S rRNA gene and QIIME2 pipeline were used.
32298317	0	55	theme	sea	120:122	arg1	Labrax					144:149	Dicentrarchus Labrax	130:149	Dicentrarchus Labrax	130:149	Assessment of dietary supplementation with galactomannan oligosaccharides and phytogenics on gut microbiota of European sea bass (Dicentrarchus Labrax) fed low fishmeal and fish oil based diet.
32298317	0	55	theme	sea	120:122	arg1	bass					124:127	European sea bass	111:127	European sea bass (Dicentrarchus Labrax)	111:150	Assessment of dietary supplementation with galactomannan oligosaccharides and phytogenics on gut microbiota of European sea bass (Dicentrarchus Labrax) fed low fishmeal and fish oil based diet.
32298317	10	56	theme	transient	1849:1857	arg1	communities					1869:1879	transient bacterial communities	1849:1879	transient bacterial communities	1849:1879	However, the composition of transient bacterial communities was not simply a mirror of feed-borne bacteria.
32298317	11	57	from	faecal	1992:1997	arg1	profile					1951:1957	the microbial profile	1937:1957	the microbial profile of feeds	1937:1966	Indeed, the microbial profile of feeds was different from both faecal and mucosa profiles.
32298317	11	57	from	faecal	1992:1997	arg1	different					1972:1980	different	1972:1980	different	1972:1980	Indeed, the microbial profile of feeds was different from both faecal and mucosa profiles.
32298317	4	58	with	%	930:930	arg1	FM					922:923	10% FM	918:923	10% FM	918:923	Three experimental diets and a control diet (plant-based formulation with 10% FM and 6% FO) were tested in a 63-days feeding trial.
32298317	12	59	theme	dietary	2050:2056	arg1	inclusion					2058:2066	the dietary inclusion	2046:2066	the dietary inclusion of GMOS (0.5%) and PHYTO (0.02%) in a low FM and FO diet	2046:2123	Our findings suggest that the dietary inclusion of GMOS (0.5%) and PHYTO (0.02%) in a low FM and FO diet induces changes in gut microbiota composition of European sea bass.
32298317	5	60	theme	rRNA	1175:1178	arg1	gene					1180:1183	16S rRNA gene	1171:1183	16S rRNA gene	1171:1183	To analyze the microbiota associated to feeds and the intestinal autochthonous (mucosa-adhered) and allochthonous (transient) microbial communities, the Illumina MiSeq platform for sequencing of 16S rRNA gene and QIIME2 pipeline were used.
32298317	0	61	theme	dietary	14:20	arg1	supplementation					22:36	dietary supplementation	14:36	dietary supplementation with galactomannan oligosaccharides and phytogenics on gut microbiota of European sea bass (Dicentrarchus Labrax)	14:150	Assessment of dietary supplementation with galactomannan oligosaccharides and phytogenics on gut microbiota of European sea bass (Dicentrarchus Labrax) fed low fishmeal and fish oil based diet.
32298317	1	62	theme	selected	284:291	arg1	additives					293:301	selected additives	284:301	selected additives that improve fish growth performance and health status	284:356	There is an increasing interest from the aquafeed industry in functional feeds containing selected additives that improve fish growth performance and health status.
32298317	3	63	theme	FO	834:835	arg1	diet					838:841	fish oil (FO) diet	824:841	fish oil (FO) diet	824:841	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	14	64	theme	beneficial	2509:2518	arg1	reduction					2572:2580	the reduction	2568:2580	the reduction	2568:2580	The main beneficial effects of GMOS and PHYTO on gut microbiota are the reduction of coliforms and Vibrionales bacteria, which include several potentially pathogenic species for fish, and the enrichment of gut microbiota composition with butyrate producer taxa.
32298317	14	64	theme	beneficial	2509:2518	arg1	effects					2520:2526	The main beneficial effects	2500:2526	The main beneficial effects of GMOS and PHYTO on gut microbiota	2500:2562	The main beneficial effects of GMOS and PHYTO on gut microbiota are the reduction of coliforms and Vibrionales bacteria, which include several potentially pathogenic species for fish, and the enrichment of gut microbiota composition with butyrate producer taxa.
32298317	3	65	theme	garlic	637:642	arg1	PHYTO					670:674	PHYTO	670:674	PHYTO	670:674	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	3	65	theme	garlic	637:642	arg1	oils					664:667	garlic and labiatae-plants oils	637:667	oils	664:667	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	3	66	from	oligosaccharides	599:614	arg1	rich					577:580	rich	577:580	rich	577:580	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	2	67	theme	organic	417:423	arg1	acids					425:429	organic acids	417:429	organic acids	417:429	Functional feed additives include probiotics, prebiotics, organic acids, and phytogenics (substances derived from plants and their extracts).
32298317	9	68	theme	transient	1656:1664	arg1	microbiota					1677:1686	transient intestinal microbiota	1656:1686	transient intestinal microbiota	1656:1686	The variation of resident gut microbiota induced by diet was lower than the variation of transient intestinal microbiota, because feedstuffs are a major source of allochthonous bacteria, which can temporarily integrate into the gut transient microbiome.
32298317	7	69	theme	reads	1392:1396	arg1	number					1382:1387	The number	1378:1387	The number of reads	1378:1396	The number of reads was significantly lower in CTRL feed than in other feeds.
32298317	7	69	theme	reads	1392:1396	arg1	lower					1416:1420	lower	1416:1420	lower	1416:1420	The number of reads was significantly lower in CTRL feed than in other feeds.
32298317	4	70	theme	10	918:919	arg1	%					920:920	%	920:920	%	920:920	Three experimental diets and a control diet (plant-based formulation with 10% FM and 6% FO) were tested in a 63-days feeding trial.
32298317	12	71	theme	sea	2183:2185	arg1	bass					2187:2190	European sea bass	2174:2190	European sea bass	2174:2190	Our findings suggest that the dietary inclusion of GMOS (0.5%) and PHYTO (0.02%) in a low FM and FO diet induces changes in gut microbiota composition of European sea bass.
32298317	13	72	theme	PHYTO	2268:2272	arg1	inclusion					2246:2254	the combined inclusion	2233:2254	the combined inclusion of GMOS and PHYTO	2233:2272	However, if on allochthonous microbiota the combined inclusion of GMOS and PHYTO showed an antagonistic effect on bactericidal activity against Vibrionales, at mucosa level, only GMOSPHYTO diet increased the relative abundance of Bacteroidales, Lactobacillales, and Clostridiales resident bacterial orders.
32298317	13	73	theme	mucosa	2353:2358	arg1	level					2360:2364	mucosa level	2353:2364	mucosa level	2353:2364	However, if on allochthonous microbiota the combined inclusion of GMOS and PHYTO showed an antagonistic effect on bactericidal activity against Vibrionales, at mucosa level, only GMOSPHYTO diet increased the relative abundance of Bacteroidales, Lactobacillales, and Clostridiales resident bacterial orders.
32298317	1	74	theme	fish	316:319	arg1	performance					328:338	fish growth performance	316:338	fish growth performance	316:338	There is an increasing interest from the aquafeed industry in functional feeds containing selected additives that improve fish growth performance and health status.
32298317	10	75	theme	feed-borne	1908:1917	arg1	bacteria					1919:1926	feed-borne bacteria	1908:1926	feed-borne bacteria	1908:1926	However, the composition of transient bacterial communities was not simply a mirror of feed-borne bacteria.
32298317	6	76	theme	experimental	1358:1369	arg1	diets					1371:1375	experimental diets	1358:1375	experimental diets	1358:1375	Metabarcoding analysis of feed-associated bacteria showed that the microbial communities of control (CTRL) feed deeply differed from those of experimental diets.
32298317	5	77	theme	transient	1091:1099	arg1	communities					1112:1122	the intestinal autochthonous (mucosa-adhered) and allochthonous (transient) microbial communities	1026:1122	communities	1112:1122	To analyze the microbiota associated to feeds and the intestinal autochthonous (mucosa-adhered) and allochthonous (transient) microbial communities, the Illumina MiSeq platform for sequencing of 16S rRNA gene and QIIME2 pipeline were used.
32298317	4	78	theme	63-days	953:959	arg1	trial					969:973	a 63-days feeding trial	951:973	a 63-days feeding trial	951:973	Three experimental diets and a control diet (plant-based formulation with 10% FM and 6% FO) were tested in a 63-days feeding trial.
32298317	12	79	theme	microbiota	2148:2157	arg1	composition					2159:2169	gut microbiota composition	2144:2169	gut microbiota composition of European sea bass	2144:2190	Our findings suggest that the dietary inclusion of GMOS (0.5%) and PHYTO (0.02%) in a low FM and FO diet induces changes in gut microbiota composition of European sea bass.
32298317	3	80	theme	labiatae-plants	648:662	arg1	PHYTO					670:674	PHYTO	670:674	PHYTO	670:674	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	3	80	theme	labiatae-plants	648:662	arg1	oils					664:667	garlic and labiatae-plants oils	637:667	oils	664:667	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	5	81	theme	microbial	1102:1110	arg1	communities					1112:1122	the intestinal autochthonous (mucosa-adhered) and allochthonous (transient) microbial communities	1026:1122	communities	1112:1122	To analyze the microbiota associated to feeds and the intestinal autochthonous (mucosa-adhered) and allochthonous (transient) microbial communities, the Illumina MiSeq platform for sequencing of 16S rRNA gene and QIIME2 pipeline were used.
32298317	9	82	theme	intestinal	1666:1675	arg1	microbiota					1677:1686	transient intestinal microbiota	1656:1686	transient intestinal microbiota	1656:1686	The variation of resident gut microbiota induced by diet was lower than the variation of transient intestinal microbiota, because feedstuffs are a major source of allochthonous bacteria, which can temporarily integrate into the gut transient microbiome.
32298317	13	83	theme	Bacteroidales	2423:2435	arg1	abundance					2410:2418	the relative abundance	2397:2418	the relative abundance of Bacteroidales, Lactobacillales, and Clostridiales resident bacterial orders	2397:2497	However, if on allochthonous microbiota the combined inclusion of GMOS and PHYTO showed an antagonistic effect on bactericidal activity against Vibrionales, at mucosa level, only GMOSPHYTO diet increased the relative abundance of Bacteroidales, Lactobacillales, and Clostridiales resident bacterial orders.
32298317	0	84	theme	oil	178:180	arg1	diet					188:191	low fishmeal and fish oil based diet	156:191	low fishmeal and fish oil based diet	156:191	Assessment of dietary supplementation with galactomannan oligosaccharides and phytogenics on gut microbiota of European sea bass (Dicentrarchus Labrax) fed low fishmeal and fish oil based diet.
32298317	2	85	theme	Functional	359:368	arg1	additives					375:383	Functional feed additives	359:383	Functional feed additives	359:383	Functional feed additives include probiotics, prebiotics, organic acids, and phytogenics (substances derived from plants and their extracts).
32298317	1	86	theme	aquafeed	235:242	arg1	industry					244:251	the aquafeed industry	231:251	the aquafeed industry	231:251	There is an increasing interest from the aquafeed industry in functional feeds containing selected additives that improve fish growth performance and health status.
32298317	13	87	theme	antagonistic	2284:2295	arg1	effect					2297:2302	an antagonistic effect	2281:2302	an antagonistic effect on bactericidal activity against Vibrionales	2281:2347	However, if on allochthonous microbiota the combined inclusion of GMOS and PHYTO showed an antagonistic effect on bactericidal activity against Vibrionales, at mucosa level, only GMOSPHYTO diet increased the relative abundance of Bacteroidales, Lactobacillales, and Clostridiales resident bacterial orders.
32298317	8	88	theme	taxonomic	1477:1485	arg1	unit					1487:1490	operational taxonomic unit	1465:1490	operational taxonomic unit	1465:1490	The OTU (operational taxonomic unit) number was instead similar between the feeds, ranging from 42 to 50 OTUs.
32298317	8	88	theme	taxonomic	1477:1485	arg1	number					1493:1498	The OTU (operational taxonomic unit) number	1456:1498	The OTU (operational taxonomic unit) number	1456:1498	The OTU (operational taxonomic unit) number was instead similar between the feeds, ranging from 42 to 50 OTUs.
32298317	14	89	theme	butyrate	2738:2745	arg1	taxa					2756:2759	butyrate producer taxa	2738:2759	butyrate producer taxa	2738:2759	The main beneficial effects of GMOS and PHYTO on gut microbiota are the reduction of coliforms and Vibrionales bacteria, which include several potentially pathogenic species for fish, and the enrichment of gut microbiota composition with butyrate producer taxa.
32298317	3	90	theme	dietary	537:543	arg1	inclusion					545:553	dietary inclusion	537:553	dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO)	537:714	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	5	91	theme	allochthonous	1076:1088	arg1	communities					1112:1122	the intestinal autochthonous (mucosa-adhered) and allochthonous (transient) microbial communities	1026:1122	communities	1112:1122	To analyze the microbiota associated to feeds and the intestinal autochthonous (mucosa-adhered) and allochthonous (transient) microbial communities, the Illumina MiSeq platform for sequencing of 16S rRNA gene and QIIME2 pipeline were used.
32298317	11	92	theme	microbial	1941:1949	arg1	profile					1951:1957	the microbial profile	1937:1957	the microbial profile of feeds	1937:1966	Indeed, the microbial profile of feeds was different from both faecal and mucosa profiles.
32298317	11	92	theme	microbial	1941:1949	arg1	different					1972:1980	different	1972:1980	different	1972:1980	Indeed, the microbial profile of feeds was different from both faecal and mucosa profiles.
32298317	14	93	theme	several	2635:2641	arg1	species					2666:2672	several potentially pathogenic species	2635:2672	several potentially pathogenic species for fish	2635:2681	The main beneficial effects of GMOS and PHYTO on gut microbiota are the reduction of coliforms and Vibrionales bacteria, which include several potentially pathogenic species for fish, and the enrichment of gut microbiota composition with butyrate producer taxa.
32298317	9	94	theme	allochthonous	1730:1742	arg1	bacteria					1744:1751	allochthonous bacteria	1730:1751	allochthonous bacteria	1730:1751	The variation of resident gut microbiota induced by diet was lower than the variation of transient intestinal microbiota, because feedstuffs are a major source of allochthonous bacteria, which can temporarily integrate into the gut transient microbiome.
32298317	3	95	theme	them	699:702	arg1	combination					684:694	a combination	682:694	a combination of them (GMOSPHYTO)	682:714	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	3	95	theme	them	699:702	arg1	oligosaccharides					599:614	galactomannan oligosaccharides	585:614	galactomannan oligosaccharides (GMOS)	585:621	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	3	95	theme	them	699:702	arg1	GMOSPHYTO					705:713	GMOSPHYTO	705:713	GMOSPHYTO	705:713	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	3	95	theme	them	699:702	arg1	mixture					626:632	a mixture	624:632	a mixture of garlic and labiatae-plants oils (PHYTO)	624:675	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	3	95	theme	them	699:702	arg1	GMOS					617:620	GMOS	617:620	GMOS	617:620	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	14	96	theme	composition	2721:2731	arg1	species					2666:2672	several potentially pathogenic species	2635:2672	several potentially pathogenic species for fish	2635:2681	The main beneficial effects of GMOS and PHYTO on gut microbiota are the reduction of coliforms and Vibrionales bacteria, which include several potentially pathogenic species for fish, and the enrichment of gut microbiota composition with butyrate producer taxa.
32298317	14	96	theme	composition	2721:2731	arg1	enrichment					2692:2701	the enrichment	2688:2701	the enrichment of gut microbiota composition	2688:2731	The main beneficial effects of GMOS and PHYTO on gut microbiota are the reduction of coliforms and Vibrionales bacteria, which include several potentially pathogenic species for fish, and the enrichment of gut microbiota composition with butyrate producer taxa.
32298317	1	97	contain	containing	273:282	arg2	additives					293:301	selected additives	284:301	selected additives that improve fish growth performance and health status	284:356	There is an increasing interest from the aquafeed industry in functional feeds containing selected additives that improve fish growth performance and health status.
32298317	1	97	contain	containing	273:282	arg1	functional					256:265	functional	256:265	functional	256:265	There is an increasing interest from the aquafeed industry in functional feeds containing selected additives that improve fish growth performance and health status.
32298317	13	98	theme	bacterial	2482:2490	arg1	orders					2492:2497	resident bacterial orders	2473:2497	resident bacterial orders	2473:2497	However, if on allochthonous microbiota the combined inclusion of GMOS and PHYTO showed an antagonistic effect on bactericidal activity against Vibrionales, at mucosa level, only GMOSPHYTO diet increased the relative abundance of Bacteroidales, Lactobacillales, and Clostridiales resident bacterial orders.
32298317	6	99	theme	feed-associated	1242:1256	arg1	bacteria					1258:1265	feed-associated bacteria	1242:1265	feed-associated bacteria	1242:1265	Metabarcoding analysis of feed-associated bacteria showed that the microbial communities of control (CTRL) feed deeply differed from those of experimental diets.
32298317	12	100	from	changes	2133:2139	arg1	composition					2159:2169	gut microbiota composition	2144:2169	gut microbiota composition of European sea bass	2144:2190	Our findings suggest that the dietary inclusion of GMOS (0.5%) and PHYTO (0.02%) in a low FM and FO diet induces changes in gut microbiota composition of European sea bass.
32298317	3	101	theme	mucilage	560:567	arg1	extract					569:575	a mucilage extract	558:575	a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO)	558:714	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	12	102	from	inclusion	2058:2066	arg1	FM					2110:2111	a low FM	2104:2111	a low FM	2104:2111	Our findings suggest that the dietary inclusion of GMOS (0.5%) and PHYTO (0.02%) in a low FM and FO diet induces changes in gut microbiota composition of European sea bass.
32298317	12	102	from	inclusion	2058:2066	arg1	diet					2120:2123	FO diet	2117:2123	FO diet	2117:2123	Our findings suggest that the dietary inclusion of GMOS (0.5%) and PHYTO (0.02%) in a low FM and FO diet induces changes in gut microbiota composition of European sea bass.
32298317	15	103	theme	bass	2894:2897	arg1	farming					2870:2876	the farming	2866:2876	the farming of European sea bass and other marine fish	2866:2919	Therefore, these functional ingredients have a great potential to be used as health-promoting agents in the farming of European sea bass and other marine fish.
32298317	15	104	theme	functional	2779:2788	arg1	ingredients					2790:2800	these functional ingredients	2773:2800	these functional ingredients	2773:2800	Therefore, these functional ingredients have a great potential to be used as health-promoting agents in the farming of European sea bass and other marine fish.
32298317	4	105	with	formulation	901:911	arg1	FM					922:923	10% FM	918:923	10% FM	918:923	Three experimental diets and a control diet (plant-based formulation with 10% FM and 6% FO) were tested in a 63-days feeding trial.
32298317	14	106	theme	bacteria	2611:2618	arg1	reduction					2572:2580	the reduction	2568:2580	the reduction	2568:2580	The main beneficial effects of GMOS and PHYTO on gut microbiota are the reduction of coliforms and Vibrionales bacteria, which include several potentially pathogenic species for fish, and the enrichment of gut microbiota composition with butyrate producer taxa.
32298317	14	106	theme	bacteria	2611:2618	arg1	effects					2520:2526	The main beneficial effects	2500:2526	The main beneficial effects of GMOS and PHYTO on gut microbiota	2500:2562	The main beneficial effects of GMOS and PHYTO on gut microbiota are the reduction of coliforms and Vibrionales bacteria, which include several potentially pathogenic species for fish, and the enrichment of gut microbiota composition with butyrate producer taxa.
32298317	0	107	theme	Dicentrarchus	130:142	arg1	Labrax					144:149	Dicentrarchus Labrax	130:149	Dicentrarchus Labrax	130:149	Assessment of dietary supplementation with galactomannan oligosaccharides and phytogenics on gut microbiota of European sea bass (Dicentrarchus Labrax) fed low fishmeal and fish oil based diet.
32298317	0	107	theme	Dicentrarchus	130:142	arg1	bass					124:127	European sea bass	111:127	European sea bass (Dicentrarchus Labrax)	111:150	Assessment of dietary supplementation with galactomannan oligosaccharides and phytogenics on gut microbiota of European sea bass (Dicentrarchus Labrax) fed low fishmeal and fish oil based diet.
32298317	15	108	theme	European	2881:2888	arg1	bass					2894:2897	European sea bass	2881:2897	European sea bass	2881:2897	Therefore, these functional ingredients have a great potential to be used as health-promoting agents in the farming of European sea bass and other marine fish.
32298317	7	109	dep	other	1443:1447	arg1	feeds					1449:1453	feeds	1449:1453	feeds	1449:1453	The number of reads was significantly lower in CTRL feed than in other feeds.
32298317	3	110	theme	galactomannan	585:597	arg1	GMOS					617:620	GMOS	617:620	GMOS	617:620	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	3	110	theme	galactomannan	585:597	arg1	oligosaccharides					599:614	galactomannan oligosaccharides	585:614	galactomannan oligosaccharides (GMOS)	585:621	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	13	111	theme	Clostridiales	2459:2471	arg1	abundance					2410:2418	the relative abundance	2397:2418	the relative abundance of Bacteroidales, Lactobacillales, and Clostridiales resident bacterial orders	2397:2497	However, if on allochthonous microbiota the combined inclusion of GMOS and PHYTO showed an antagonistic effect on bactericidal activity against Vibrionales, at mucosa level, only GMOSPHYTO diet increased the relative abundance of Bacteroidales, Lactobacillales, and Clostridiales resident bacterial orders.
32298317	14	112	theme	gut	2706:2708	arg1	composition					2721:2731	gut microbiota composition	2706:2731	gut microbiota composition	2706:2731	The main beneficial effects of GMOS and PHYTO on gut microbiota are the reduction of coliforms and Vibrionales bacteria, which include several potentially pathogenic species for fish, and the enrichment of gut microbiota composition with butyrate producer taxa.
32298317	0	113	theme	low	156:158	arg1	fishmeal					160:167	low fishmeal	156:167	low fishmeal	156:167	Assessment of dietary supplementation with galactomannan oligosaccharides and phytogenics on gut microbiota of European sea bass (Dicentrarchus Labrax) fed low fishmeal and fish oil based diet.
32298317	3	114	theme	rich	577:580	arg1	extract					569:575	a mucilage extract	558:575	a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO)	558:714	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	9	115	theme	transient	1799:1807	arg1	microbiome					1809:1818	the gut transient microbiome	1791:1818	the gut transient microbiome	1791:1818	The variation of resident gut microbiota induced by diet was lower than the variation of transient intestinal microbiota, because feedstuffs are a major source of allochthonous bacteria, which can temporarily integrate into the gut transient microbiome.
32298317	3	116	theme	gut	720:722	arg1	composition					735:745	gut microbiota composition	720:745	gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet	720:841	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	13	117	theme	combined	2237:2244	arg1	inclusion					2246:2254	the combined inclusion	2233:2254	the combined inclusion of GMOS and PHYTO	2233:2272	However, if on allochthonous microbiota the combined inclusion of GMOS and PHYTO showed an antagonistic effect on bactericidal activity against Vibrionales, at mucosa level, only GMOSPHYTO diet increased the relative abundance of Bacteroidales, Lactobacillales, and Clostridiales resident bacterial orders.
32298317	3	118	theme	low	802:804	arg1	FM					816:817	FM	816:817	FM	816:817	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	3	118	theme	low	802:804	arg1	fishmeal					806:813	a low fishmeal	800:813	a low fishmeal (FM)	800:818	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	12	119	theme	low	2106:2108	arg1	FM					2110:2111	a low FM	2104:2111	a low FM	2104:2111	Our findings suggest that the dietary inclusion of GMOS (0.5%) and PHYTO (0.02%) in a low FM and FO diet induces changes in gut microbiota composition of European sea bass.
32298317	3	120	theme	bass	763:766	arg1	composition					735:745	gut microbiota composition	720:745	gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet	720:841	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	15	121	contain	have	2802:2805	arg1	ingredients					2790:2800	these functional ingredients	2773:2800	these functional ingredients	2773:2800	Therefore, these functional ingredients have a great potential to be used as health-promoting agents in the farming of European sea bass and other marine fish.
32298317	15	121	contain	have	2802:2805	arg2	potential					2815:2823	a great potential	2807:2823	a great potential to be used as health-promoting agents in the farming of European sea bass and other marine fish	2807:2919	Therefore, these functional ingredients have a great potential to be used as health-promoting agents in the farming of European sea bass and other marine fish.
32298317	9	122	theme	resident	1584:1591	arg1	microbiota					1597:1606	resident gut microbiota	1584:1606	resident gut microbiota induced by diet	1584:1622	The variation of resident gut microbiota induced by diet was lower than the variation of transient intestinal microbiota, because feedstuffs are a major source of allochthonous bacteria, which can temporarily integrate into the gut transient microbiome.
32298317	1	123	theme	increasing	206:215	arg1	interest					217:224	an increasing interest	203:224	an increasing interest from the aquafeed industry in functional feeds containing selected additives that improve fish growth performance and health status	203:356	There is an increasing interest from the aquafeed industry in functional feeds containing selected additives that improve fish growth performance and health status.
32298317	3	124	from	effects	526:532	arg1	composition					735:745	gut microbiota composition	720:745	gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet	720:841	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	3	125	theme	European	750:757	arg1	bass					763:766	European sea bass	750:766	European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet	750:841	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	3	125	theme	European	750:757	arg1	labrax					783:788	Dicentrarchus labrax	769:788	Dicentrarchus labrax	769:788	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	6	126	theme	bacteria	1258:1265	arg1	analysis					1230:1237	Metabarcoding analysis	1216:1237	Metabarcoding analysis of feed-associated bacteria	1216:1265	Metabarcoding analysis of feed-associated bacteria showed that the microbial communities of control (CTRL) feed deeply differed from those of experimental diets.
32298317	15	127	theme	fish	2916:2919	arg1	farming					2870:2876	the farming	2866:2876	the farming of European sea bass and other marine fish	2866:2919	Therefore, these functional ingredients have a great potential to be used as health-promoting agents in the farming of European sea bass and other marine fish.
32298317	9	128	theme	microbiota	1597:1606	arg1	lower					1628:1632	lower	1628:1632	lower	1628:1632	The variation of resident gut microbiota induced by diet was lower than the variation of transient intestinal microbiota, because feedstuffs are a major source of allochthonous bacteria, which can temporarily integrate into the gut transient microbiome.
32298317	9	128	theme	microbiota	1597:1606	arg1	variation					1571:1579	The variation	1567:1579	The variation of resident gut microbiota induced by diet	1567:1622	The variation of resident gut microbiota induced by diet was lower than the variation of transient intestinal microbiota, because feedstuffs are a major source of allochthonous bacteria, which can temporarily integrate into the gut transient microbiome.
32298317	5	129	theme	Illumina	1129:1136	arg1	platform					1144:1151	the Illumina MiSeq platform	1125:1151	the Illumina MiSeq platform for sequencing of 16S rRNA gene and QIIME2 pipeline	1125:1203	To analyze the microbiota associated to feeds and the intestinal autochthonous (mucosa-adhered) and allochthonous (transient) microbial communities, the Illumina MiSeq platform for sequencing of 16S rRNA gene and QIIME2 pipeline were used.
32298317	0	130	theme	European	111:118	arg1	Labrax					144:149	Dicentrarchus Labrax	130:149	Dicentrarchus Labrax	130:149	Assessment of dietary supplementation with galactomannan oligosaccharides and phytogenics on gut microbiota of European sea bass (Dicentrarchus Labrax) fed low fishmeal and fish oil based diet.
32298317	0	130	theme	European	111:118	arg1	bass					124:127	European sea bass	111:127	European sea bass (Dicentrarchus Labrax)	111:150	Assessment of dietary supplementation with galactomannan oligosaccharides and phytogenics on gut microbiota of European sea bass (Dicentrarchus Labrax) fed low fishmeal and fish oil based diet.
32298317	15	131	theme	other	2903:2907	arg1	fish					2916:2919	other marine fish	2903:2919	other marine fish	2903:2919	Therefore, these functional ingredients have a great potential to be used as health-promoting agents in the farming of European sea bass and other marine fish.
32298317	5	132	theme	QIIME2	1189:1194	arg1	pipeline					1196:1203	QIIME2 pipeline	1189:1203	QIIME2 pipeline	1189:1203	To analyze the microbiota associated to feeds and the intestinal autochthonous (mucosa-adhered) and allochthonous (transient) microbial communities, the Illumina MiSeq platform for sequencing of 16S rRNA gene and QIIME2 pipeline were used.
32298317	3	133	theme	Dicentrarchus	769:781	arg1	bass					763:766	European sea bass	750:766	European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet	750:841	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	3	133	theme	Dicentrarchus	769:781	arg1	labrax					783:788	Dicentrarchus labrax	769:788	Dicentrarchus labrax	769:788	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	0	134	theme	bass	124:127	arg1	microbiota					97:106	gut microbiota	93:106	gut microbiota of European sea bass (Dicentrarchus Labrax)	93:150	Assessment of dietary supplementation with galactomannan oligosaccharides and phytogenics on gut microbiota of European sea bass (Dicentrarchus Labrax) fed low fishmeal and fish oil based diet.
32298317	2	135	dep	phytogenics	436:446	arg1	substances					449:458	substances	449:458	substances derived from plants and their extracts	449:497	Functional feed additives include probiotics, prebiotics, organic acids, and phytogenics (substances derived from plants and their extracts).
32298317	14	136	theme	PHYTO	2540:2544	arg1	reduction					2572:2580	the reduction	2568:2580	the reduction	2568:2580	The main beneficial effects of GMOS and PHYTO on gut microbiota are the reduction of coliforms and Vibrionales bacteria, which include several potentially pathogenic species for fish, and the enrichment of gut microbiota composition with butyrate producer taxa.
32298317	14	136	theme	PHYTO	2540:2544	arg1	effects					2520:2526	The main beneficial effects	2500:2526	The main beneficial effects of GMOS and PHYTO on gut microbiota	2500:2562	The main beneficial effects of GMOS and PHYTO on gut microbiota are the reduction of coliforms and Vibrionales bacteria, which include several potentially pathogenic species for fish, and the enrichment of gut microbiota composition with butyrate producer taxa.
32298317	10	137	theme	communities	1869:1879	arg1	composition					1834:1844	the composition	1830:1844	the composition of transient bacterial communities	1830:1879	However, the composition of transient bacterial communities was not simply a mirror of feed-borne bacteria.
32298317	10	137	theme	communities	1869:1879	arg1	a					1896:1896	a	1896:1896	a	1896:1896	However, the composition of transient bacterial communities was not simply a mirror of feed-borne bacteria.
32298317	5	138	used	used	1210:1213	arg2	platform					1144:1151	the Illumina MiSeq platform	1125:1151	the Illumina MiSeq platform for sequencing of 16S rRNA gene and QIIME2 pipeline	1125:1203	To analyze the microbiota associated to feeds and the intestinal autochthonous (mucosa-adhered) and allochthonous (transient) microbial communities, the Illumina MiSeq platform for sequencing of 16S rRNA gene and QIIME2 pipeline were used.
32298317	12	139	theme	GMOS	2071:2074	arg1	inclusion					2058:2066	the dietary inclusion	2046:2066	the dietary inclusion of GMOS (0.5%) and PHYTO (0.02%) in a low FM and FO diet	2046:2123	Our findings suggest that the dietary inclusion of GMOS (0.5%) and PHYTO (0.02%) in a low FM and FO diet induces changes in gut microbiota composition of European sea bass.
32298317	6	140	theme	Metabarcoding	1216:1228	arg1	analysis					1230:1237	Metabarcoding analysis	1216:1237	Metabarcoding analysis of feed-associated bacteria	1216:1265	Metabarcoding analysis of feed-associated bacteria showed that the microbial communities of control (CTRL) feed deeply differed from those of experimental diets.
32298317	3	141	theme	fish	824:827	arg1	diet					838:841	fish oil (FO) diet	824:841	fish oil (FO) diet	824:841	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	14	142	theme	GMOS	2531:2534	arg1	reduction					2572:2580	the reduction	2568:2580	the reduction	2568:2580	The main beneficial effects of GMOS and PHYTO on gut microbiota are the reduction of coliforms and Vibrionales bacteria, which include several potentially pathogenic species for fish, and the enrichment of gut microbiota composition with butyrate producer taxa.
32298317	14	142	theme	GMOS	2531:2534	arg1	effects					2520:2526	The main beneficial effects	2500:2526	The main beneficial effects of GMOS and PHYTO on gut microbiota	2500:2562	The main beneficial effects of GMOS and PHYTO on gut microbiota are the reduction of coliforms and Vibrionales bacteria, which include several potentially pathogenic species for fish, and the enrichment of gut microbiota composition with butyrate producer taxa.
32298317	4	143	theme	%	920:920	arg1	FM					922:923	10% FM	918:923	10% FM	918:923	Three experimental diets and a control diet (plant-based formulation with 10% FM and 6% FO) were tested in a 63-days feeding trial.
32298317	3	144	from	rich	577:580	arg1	combination					684:694	a combination	682:694	a combination of them (GMOSPHYTO)	682:714	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	3	144	from	rich	577:580	arg1	oligosaccharides					599:614	galactomannan oligosaccharides	585:614	galactomannan oligosaccharides (GMOS)	585:621	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	3	144	from	rich	577:580	arg1	GMOSPHYTO					705:713	GMOSPHYTO	705:713	GMOSPHYTO	705:713	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	3	144	from	rich	577:580	arg1	mixture					626:632	a mixture	624:632	a mixture of garlic and labiatae-plants oils (PHYTO)	624:675	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	3	144	from	rich	577:580	arg1	GMOS					617:620	GMOS	617:620	GMOS	617:620	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	0	145	theme	supplementation	22:36	arg1	Assessment					0:9	Assessment	0:9	Assessment of dietary supplementation with galactomannan oligosaccharides and phytogenics on gut microbiota of European sea bass (Dicentrarchus Labrax)	0:150	Assessment of dietary supplementation with galactomannan oligosaccharides and phytogenics on gut microbiota of European sea bass (Dicentrarchus Labrax) fed low fishmeal and fish oil based diet.
32298317	13	146	theme	GMOSPHYTO	2372:2380	arg1	diet					2382:2385	only GMOSPHYTO diet	2367:2385	only GMOSPHYTO diet	2367:2385	However, if on allochthonous microbiota the combined inclusion of GMOS and PHYTO showed an antagonistic effect on bactericidal activity against Vibrionales, at mucosa level, only GMOSPHYTO diet increased the relative abundance of Bacteroidales, Lactobacillales, and Clostridiales resident bacterial orders.
32298317	5	147	theme	gene	1180:1183	arg1	sequencing					1157:1166	sequencing	1157:1166	sequencing of 16S rRNA gene and QIIME2 pipeline	1157:1203	To analyze the microbiota associated to feeds and the intestinal autochthonous (mucosa-adhered) and allochthonous (transient) microbial communities, the Illumina MiSeq platform for sequencing of 16S rRNA gene and QIIME2 pipeline were used.
32298317	0	148	theme	galactomannan	43:55	arg1	oligosaccharides					57:72	galactomannan oligosaccharides	43:72	galactomannan oligosaccharides	43:72	Assessment of dietary supplementation with galactomannan oligosaccharides and phytogenics on gut microbiota of European sea bass (Dicentrarchus Labrax) fed low fishmeal and fish oil based diet.
32298317	14	149	theme	main	2504:2507	arg1	reduction					2572:2580	the reduction	2568:2580	the reduction	2568:2580	The main beneficial effects of GMOS and PHYTO on gut microbiota are the reduction of coliforms and Vibrionales bacteria, which include several potentially pathogenic species for fish, and the enrichment of gut microbiota composition with butyrate producer taxa.
32298317	14	149	theme	main	2504:2507	arg1	effects					2520:2526	The main beneficial effects	2500:2526	The main beneficial effects of GMOS and PHYTO on gut microbiota	2500:2562	The main beneficial effects of GMOS and PHYTO on gut microbiota are the reduction of coliforms and Vibrionales bacteria, which include several potentially pathogenic species for fish, and the enrichment of gut microbiota composition with butyrate producer taxa.
32298317	4	150	theme	plant-based	889:899	arg1	formulation					901:911	plant-based formulation	889:911	plant-based formulation with 10% FM	889:923	Three experimental diets and a control diet (plant-based formulation with 10% FM and 6% FO) were tested in a 63-days feeding trial.
32298317	12	151	theme	bass	2187:2190	arg1	composition					2159:2169	gut microbiota composition	2144:2169	gut microbiota composition of European sea bass	2144:2190	Our findings suggest that the dietary inclusion of GMOS (0.5%) and PHYTO (0.02%) in a low FM and FO diet induces changes in gut microbiota composition of European sea bass.
32298317	12	152	theme	European	2174:2181	arg1	bass					2187:2190	European sea bass	2174:2190	European sea bass	2174:2190	Our findings suggest that the dietary inclusion of GMOS (0.5%) and PHYTO (0.02%) in a low FM and FO diet induces changes in gut microbiota composition of European sea bass.
32298317	0	153	with	supplementation	22:36	arg1	phytogenics					78:88	phytogenics	78:88	phytogenics	78:88	Assessment of dietary supplementation with galactomannan oligosaccharides and phytogenics on gut microbiota of European sea bass (Dicentrarchus Labrax) fed low fishmeal and fish oil based diet.
32298317	0	153	with	supplementation	22:36	arg1	oligosaccharides					57:72	galactomannan oligosaccharides	43:72	galactomannan oligosaccharides	43:72	Assessment of dietary supplementation with galactomannan oligosaccharides and phytogenics on gut microbiota of European sea bass (Dicentrarchus Labrax) fed low fishmeal and fish oil based diet.
32298317	13	154	theme	relative	2401:2408	arg1	abundance					2410:2418	the relative abundance	2397:2418	the relative abundance of Bacteroidales, Lactobacillales, and Clostridiales resident bacterial orders	2397:2497	However, if on allochthonous microbiota the combined inclusion of GMOS and PHYTO showed an antagonistic effect on bactericidal activity against Vibrionales, at mucosa level, only GMOSPHYTO diet increased the relative abundance of Bacteroidales, Lactobacillales, and Clostridiales resident bacterial orders.
32298317	6	155	theme	microbial	1283:1291	arg1	communities					1293:1303	the microbial communities	1279:1303	the microbial communities of control (CTRL)	1279:1321	Metabarcoding analysis of feed-associated bacteria showed that the microbial communities of control (CTRL) feed deeply differed from those of experimental diets.
32298317	6	156	dep	feed	1323:1326	arg1	differed					1335:1342	differed	1335:1342	feed deeply differed from those of experimental diets	1323:1375	Metabarcoding analysis of feed-associated bacteria showed that the microbial communities of control (CTRL) feed deeply differed from those of experimental diets.
32298317	15	157	theme	health-promoting	2839:2854	arg1	agents					2856:2861	health-promoting agents	2839:2861	health-promoting agents in the farming of European sea bass and other marine fish	2839:2919	Therefore, these functional ingredients have a great potential to be used as health-promoting agents in the farming of European sea bass and other marine fish.
32298317	9	158	theme	microbiota	1677:1686	arg1	variation					1643:1651	the variation	1639:1651	the variation of transient intestinal microbiota	1639:1686	The variation of resident gut microbiota induced by diet was lower than the variation of transient intestinal microbiota, because feedstuffs are a major source of allochthonous bacteria, which can temporarily integrate into the gut transient microbiome.
32298317	3	159	theme	oils	664:667	arg1	combination					684:694	a combination	682:694	a combination of them (GMOSPHYTO)	682:714	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	3	159	theme	oils	664:667	arg1	oligosaccharides					599:614	galactomannan oligosaccharides	585:614	galactomannan oligosaccharides (GMOS)	585:621	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	3	159	theme	oils	664:667	arg1	GMOSPHYTO					705:713	GMOSPHYTO	705:713	GMOSPHYTO	705:713	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	3	159	theme	oils	664:667	arg1	mixture					626:632	a mixture	624:632	a mixture of garlic and labiatae-plants oils (PHYTO)	624:675	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	3	159	theme	oils	664:667	arg1	GMOS					617:620	GMOS	617:620	GMOS	617:620	This study evaluated the effects of dietary inclusion of a mucilage extract rich in galactomannan oligosaccharides (GMOS), a mixture of garlic and labiatae-plants oils (PHYTO), and a combination of them (GMOSPHYTO), on gut microbiota composition of European sea bass (Dicentrarchus labrax) fed with a low fishmeal (FM) and fish oil (FO) diet.
32298317	1	160	theme	growth	321:326	arg1	performance					328:338	fish growth performance	316:338	fish growth performance	316:338	There is an increasing interest from the aquafeed industry in functional feeds containing selected additives that improve fish growth performance and health status.
32298317	8	161	dep	50	1558:1559	arg1	to					1555:1556	to	1555:1556	to	1555:1556	The OTU (operational taxonomic unit) number was instead similar between the feeds, ranging from 42 to 50 OTUs.
32298317	8	162	theme	OTU	1460:1462	arg1	similar					1512:1518	similar	1512:1518	similar	1512:1518	The OTU (operational taxonomic unit) number was instead similar between the feeds, ranging from 42 to 50 OTUs.
32298317	8	162	theme	OTU	1460:1462	arg1	unit					1487:1490	operational taxonomic unit	1465:1490	operational taxonomic unit	1465:1490	The OTU (operational taxonomic unit) number was instead similar between the feeds, ranging from 42 to 50 OTUs.
32298317	8	162	theme	OTU	1460:1462	arg1	number					1493:1498	The OTU (operational taxonomic unit) number	1456:1498	The OTU (operational taxonomic unit) number	1456:1498	The OTU (operational taxonomic unit) number was instead similar between the feeds, ranging from 42 to 50 OTUs.
32298317	13	163	theme	allochthonous	2208:2220	arg1	microbiota					2222:2231	allochthonous microbiota	2208:2231	allochthonous microbiota	2208:2231	However, if on allochthonous microbiota the combined inclusion of GMOS and PHYTO showed an antagonistic effect on bactericidal activity against Vibrionales, at mucosa level, only GMOSPHYTO diet increased the relative abundance of Bacteroidales, Lactobacillales, and Clostridiales resident bacterial orders.
32298317	15	164	theme	marine	2909:2914	arg1	fish					2916:2919	other marine fish	2903:2919	other marine fish	2903:2919	Therefore, these functional ingredients have a great potential to be used as health-promoting agents in the farming of European sea bass and other marine fish.
32298317	8	165	theme	operational	1465:1475	arg1	unit					1487:1490	operational taxonomic unit	1465:1490	operational taxonomic unit	1465:1490	The OTU (operational taxonomic unit) number was instead similar between the feeds, ranging from 42 to 50 OTUs.
32298317	8	165	theme	operational	1465:1475	arg1	number					1493:1498	The OTU (operational taxonomic unit) number	1456:1498	The OTU (operational taxonomic unit) number	1456:1498	The OTU (operational taxonomic unit) number was instead similar between the feeds, ranging from 42 to 50 OTUs.
32298317	11	166	from	profiles	2010:2017	arg1	profile					1951:1957	the microbial profile	1937:1957	the microbial profile of feeds	1937:1966	Indeed, the microbial profile of feeds was different from both faecal and mucosa profiles.
32298317	11	166	from	profiles	2010:2017	arg1	different					1972:1980	different	1972:1980	different	1972:1980	Indeed, the microbial profile of feeds was different from both faecal and mucosa profiles.
32298317	2	167	theme	feed	370:373	arg1	additives					375:383	Functional feed additives	359:383	Functional feed additives	359:383	Functional feed additives include probiotics, prebiotics, organic acids, and phytogenics (substances derived from plants and their extracts).
34855861	9	0	theme	magnetic	1575:1582	arg1	NMR					1595:1597	NMR	1595:1597	NMR	1595:1597	The microbiota composition in fecal, ileum, colon and adipose tissue was analyzed using 16S rDNA sequencing, fecal and liver metabolomics were performed by nuclear magnetic resonance (NMR) spectroscopy.
34855861	9	0	theme	magnetic	1575:1582	arg1	resonance					1584:1592	nuclear magnetic resonance	1567:1592	nuclear magnetic resonance (NMR) spectroscopy	1567:1611	The microbiota composition in fecal, ileum, colon and adipose tissue was analyzed using 16S rDNA sequencing, fecal and liver metabolomics were performed by nuclear magnetic resonance (NMR) spectroscopy.
34855861	8	1	theme	ileum	1338:1342	arg1	end					1313:1315	end	1313:1315	end of the intervention, ileum and colon mucosa	1313:1359	At end of the intervention, ileum and colon mucosa, adipose tissue and liver samples were collected.
34855861	2	2	theme	bacterial	342:350	arg1	species					352:358	certain bacterial species	334:358	certain bacterial species	334:358	The past two decades of research have raised gut microbiota composition as a contributing factor to the development of obesity, and higher abundance of certain bacterial species has been linked to the lean phenotype, such as Akkermansia muciniphila.
34855861	5	3	from	composition	892:902	arg1	tract					928:932	the gastrointestinal tract	907:932	the gastrointestinal tract	907:932	lactis 420 (B420), during high fat diet (HFD; 60 kcal% fat) affects microbiota composition in the gastrointestinal tract and adipose tissue, and metabolite levels in gut and liver.
34855861	5	3	from	composition	892:902	arg1	liver					987:991	liver	987:991	liver	987:991	lactis 420 (B420), during high fat diet (HFD; 60 kcal% fat) affects microbiota composition in the gastrointestinal tract and adipose tissue, and metabolite levels in gut and liver.
34855861	5	3	from	composition	892:902	arg1	tissue					946:951	adipose tissue	938:951	adipose tissue	938:951	lactis 420 (B420), during high fat diet (HFD; 60 kcal% fat) affects microbiota composition in the gastrointestinal tract and adipose tissue, and metabolite levels in gut and liver.
34855861	5	3	from	composition	892:902	arg1	gut					979:981	gut	979:981	gut	979:981	lactis 420 (B420), during high fat diet (HFD; 60 kcal% fat) affects microbiota composition in the gastrointestinal tract and adipose tissue, and metabolite levels in gut and liver.
34855861	8	4	theme	colon	1348:1352	arg1	mucosa					1354:1359	colon mucosa	1348:1359	colon mucosa	1348:1359	At end of the intervention, ileum and colon mucosa, adipose tissue and liver samples were collected.
34855861	6	5	from	study	1002:1006	arg1	split					1037:1041	split	1037:1041	split	1037:1041	In this study C57Bl/6J mice (N = 200) were split in five treatments and daily gavaged: 1) Normal control (NC); 2) HFD; 3) HFD + PDX; 4) HFD + B420 or 5) HFD + PDX + B420 (HFD+S).
34855861	3	6	theme	community	517:525	arg1	alterations					527:537	microbial community alterations	507:537	microbial community alterations	507:537	The ability of pre- and probiotics to affect metabolic health could be via microbial community alterations and subsequently changes in metabolite profiles, modulating for example host energy balance via complex signaling pathways.
34855861	7	7	theme	glucose-tolerance	1215:1231	arg1	test					1233:1236	intraperitoneal glucose-tolerance test	1199:1236	intraperitoneal glucose-tolerance test (IPGTT)	1199:1244	At six weeks of treatment intraperitoneal glucose-tolerance test (IPGTT) was performed, and feces were collected at weeks 0, 3, 6 and 9.
34855861	7	7	theme	glucose-tolerance	1215:1231	arg1	IPGTT					1239:1243	IPGTT	1239:1243	IPGTT	1239:1243	At six weeks of treatment intraperitoneal glucose-tolerance test (IPGTT) was performed, and feces were collected at weeks 0, 3, 6 and 9.
34855861	10	8	theme	body	1661:1664	arg1	gain					1673:1676	body weight gain	1661:1676	body weight gain	1661:1676	It was found that HFD+PDX intervention reduced body weight gain and hepatic fat compared to HFD.
34855861	9	9	dep	fecal	1520:1524	arg1	metabolomics					1536:1547	metabolomics	1536:1547	metabolomics	1536:1547	The microbiota composition in fecal, ileum, colon and adipose tissue was analyzed using 16S rDNA sequencing, fecal and liver metabolomics were performed by nuclear magnetic resonance (NMR) spectroscopy.
34855861	3	10	theme	metabolite	567:576	arg1	profiles					578:585	metabolite profiles	567:585	metabolite profiles	567:585	The ability of pre- and probiotics to affect metabolic health could be via microbial community alterations and subsequently changes in metabolite profiles, modulating for example host energy balance via complex signaling pathways.
34855861	5	11	theme	high	839:842	arg1	diet					848:851	high fat diet	839:851	high fat diet (HFD; 60 kcal% fat)	839:871	lactis 420 (B420), during high fat diet (HFD; 60 kcal% fat) affects microbiota composition in the gastrointestinal tract and adipose tissue, and metabolite levels in gut and liver.
34855861	12	12	theme	fecal	1862:1866	arg1	levels					1885:1890	fecal, lleum and colon levels	1862:1890	fecal, lleum and colon levels of Akkermansia	1862:1905	Furthermore, by the inclusion of PDX, fecal, lleum and colon levels of Akkermansia were increased and liver health was improved as the detoxification capacity and levels of methyl-donors were increased.
34855861	11	13	theme	adipose	1731:1737	arg1	MAT					1747:1749	MAT	1747:1749	MAT	1747:1749	Sequencing the mice adipose tissue (MAT) identified Akkermansia and its prevalence was increased in HFD+S group.
34855861	11	13	theme	adipose	1731:1737	arg1	tissue					1739:1744	the mice adipose tissue	1722:1744	Sequencing the mice adipose tissue (MAT)	1711:1750	Sequencing the mice adipose tissue (MAT) identified Akkermansia and its prevalence was increased in HFD+S group.
34855861	10	14	theme	hepatic	1682:1688	arg1	fat					1690:1692	hepatic fat	1682:1692	hepatic fat	1682:1692	It was found that HFD+PDX intervention reduced body weight gain and hepatic fat compared to HFD.
34855861	2	15	theme	gut	227:229	arg1	factor					272:277	a contributing factor	257:277	a contributing factor to the development of obesity	257:307	The past two decades of research have raised gut microbiota composition as a contributing factor to the development of obesity, and higher abundance of certain bacterial species has been linked to the lean phenotype, such as Akkermansia muciniphila.
34855861	2	15	theme	gut	227:229	arg1	composition					242:252	gut microbiota composition	227:252	gut microbiota composition	227:252	The past two decades of research have raised gut microbiota composition as a contributing factor to the development of obesity, and higher abundance of certain bacterial species has been linked to the lean phenotype, such as Akkermansia muciniphila.
34855861	12	16	theme	methyl-donors	1997:2009	arg1	levels					1987:1992	levels	1987:1992	levels of methyl-donors	1987:2009	Furthermore, by the inclusion of PDX, fecal, lleum and colon levels of Akkermansia were increased and liver health was improved as the detoxification capacity and levels of methyl-donors were increased.
34855861	1	17	theme	liver	121:125	arg1	health					127:132	liver health	121:132	liver health in a multi-compartmental obesogenic mice study	121:179	lactis 420 drives the prevalence of Akkermansia and improves liver health in a multi-compartmental obesogenic mice study.
34855861	2	18	theme	contributing	259:270	arg1	factor					272:277	a contributing factor	257:277	a contributing factor to the development of obesity	257:307	The past two decades of research have raised gut microbiota composition as a contributing factor to the development of obesity, and higher abundance of certain bacterial species has been linked to the lean phenotype, such as Akkermansia muciniphila.
34855861	2	18	theme	contributing	259:270	arg1	composition					242:252	gut microbiota composition	227:252	gut microbiota composition	227:252	The past two decades of research have raised gut microbiota composition as a contributing factor to the development of obesity, and higher abundance of certain bacterial species has been linked to the lean phenotype, such as Akkermansia muciniphila.
34855861	6	19	dep	control	1091:1097	arg1	B420					1136:1139	B420	1136:1139	B420	1136:1139	In this study C57Bl/6J mice (N = 200) were split in five treatments and daily gavaged: 1) Normal control (NC); 2) HFD; 3) HFD + PDX; 4) HFD + B420 or 5) HFD + PDX + B420 (HFD+S).
34855861	6	19	dep	control	1091:1097	arg1	HFD					1108:1110	2) HFD	1105:1110	1) Normal control (NC); 2) HFD; 3) HFD + PDX; 4) HFD + B420 or 5) HFD + PDX + B420 (HFD+S)	1081:1170	In this study C57Bl/6J mice (N = 200) were split in five treatments and daily gavaged: 1) Normal control (NC); 2) HFD; 3) HFD + PDX; 4) HFD + B420 or 5) HFD + PDX + B420 (HFD+S).
34855861	6	19	dep	control	1091:1097	arg1	PDX					1122:1124	PDX	1122:1124	PDX	1122:1124	In this study C57Bl/6J mice (N = 200) were split in five treatments and daily gavaged: 1) Normal control (NC); 2) HFD; 3) HFD + PDX; 4) HFD + B420 or 5) HFD + PDX + B420 (HFD+S).
34855861	6	19	dep	control	1091:1097	arg1	HFD					1147:1149	5) HFD + PDX + B420 (HFD+S)	1144:1170	5) HFD + PDX + B420 (HFD+S)	1144:1170	In this study C57Bl/6J mice (N = 200) were split in five treatments and daily gavaged: 1) Normal control (NC); 2) HFD; 3) HFD + PDX; 4) HFD + B420 or 5) HFD + PDX + B420 (HFD+S).
34855861	6	19	dep	control	1091:1097	arg1	1					1081:1081	1	1081:1081	1	1081:1081	In this study C57Bl/6J mice (N = 200) were split in five treatments and daily gavaged: 1) Normal control (NC); 2) HFD; 3) HFD + PDX; 4) HFD + B420 or 5) HFD + PDX + B420 (HFD+S).
34855861	6	19	dep	control	1091:1097	arg1	HFD					1116:1118	HFD	1116:1118	HFD	1116:1118	In this study C57Bl/6J mice (N = 200) were split in five treatments and daily gavaged: 1) Normal control (NC); 2) HFD; 3) HFD + PDX; 4) HFD + B420 or 5) HFD + PDX + B420 (HFD+S).
34855861	6	19	dep	control	1091:1097	arg1	HFD					1130:1132	HFD	1130:1132	HFD	1130:1132	In this study C57Bl/6J mice (N = 200) were split in five treatments and daily gavaged: 1) Normal control (NC); 2) HFD; 3) HFD + PDX; 4) HFD + B420 or 5) HFD + PDX + B420 (HFD+S).
34855861	1	20	theme	Akkermansia	96:106	arg1	prevalence					82:91	the prevalence	78:91	the prevalence of Akkermansia	78:106	lactis 420 drives the prevalence of Akkermansia and improves liver health in a multi-compartmental obesogenic mice study.
34855861	13	21	theme	adipose	2125:2131	arg1	tissue					2133:2138	adipose tissue	2125:2138	adipose tissue	2125:2138	These new results demonstrate how PDX and B420 can affect the interactions between gut, liver and adipose tissue.
34855861	5	22	theme	%	866:866	arg1	fat					868:870	60 kcal% fat	859:870	HFD; 60 kcal% fat	854:870	lactis 420 (B420), during high fat diet (HFD; 60 kcal% fat) affects microbiota composition in the gastrointestinal tract and adipose tissue, and metabolite levels in gut and liver.
34855861	4	23	theme	mice	679:682	arg1	study					684:688	this mice study	674:688	this mice study	674:688	The aim of this mice study was to determine how administration of a prebiotic fiber, polydextrose (PDX) and a probiotic Bifidobacterium animalis ssp.
34855861	3	24	from	changes	556:562	arg1	profiles					578:585	metabolite profiles	567:585	metabolite profiles	567:585	The ability of pre- and probiotics to affect metabolic health could be via microbial community alterations and subsequently changes in metabolite profiles, modulating for example host energy balance via complex signaling pathways.
34855861	3	25	theme	host	611:614	arg1	balance					623:629	host energy balance	611:629	host energy balance	611:629	The ability of pre- and probiotics to affect metabolic health could be via microbial community alterations and subsequently changes in metabolite profiles, modulating for example host energy balance via complex signaling pathways.
34855861	1	26	theme	multi-compartmental	139:157	arg1	mice					170:173	multi-compartmental obesogenic mice	139:173	a multi-compartmental obesogenic mice study	137:179	lactis 420 drives the prevalence of Akkermansia and improves liver health in a multi-compartmental obesogenic mice study.
34855861	1	27	theme	mice	170:173	arg1	study					175:179	a multi-compartmental obesogenic mice study	137:179	a multi-compartmental obesogenic mice study	137:179	lactis 420 drives the prevalence of Akkermansia and improves liver health in a multi-compartmental obesogenic mice study.
34855861	2	28	theme	past	186:189	arg1	decades					195:201	The past two decades	182:201	The past two decades of research	182:213	The past two decades of research have raised gut microbiota composition as a contributing factor to the development of obesity, and higher abundance of certain bacterial species has been linked to the lean phenotype, such as Akkermansia muciniphila.
34855861	9	29	theme	adipose	1465:1471	arg1	tissue					1473:1478	adipose tissue	1465:1478	adipose tissue	1465:1478	The microbiota composition in fecal, ileum, colon and adipose tissue was analyzed using 16S rDNA sequencing, fecal and liver metabolomics were performed by nuclear magnetic resonance (NMR) spectroscopy.
34855861	11	30	dep	Sequencing	1711:1720	arg1	MAT					1747:1749	MAT	1747:1749	MAT	1747:1749	Sequencing the mice adipose tissue (MAT) identified Akkermansia and its prevalence was increased in HFD+S group.
34855861	11	30	dep	Sequencing	1711:1720	arg1	tissue					1739:1744	the mice adipose tissue	1722:1744	Sequencing the mice adipose tissue (MAT)	1711:1750	Sequencing the mice adipose tissue (MAT) identified Akkermansia and its prevalence was increased in HFD+S group.
34855861	4	31	theme	prebiotic	731:739	arg1	fiber					741:745	a prebiotic fiber	729:745	a prebiotic fiber	729:745	The aim of this mice study was to determine how administration of a prebiotic fiber, polydextrose (PDX) and a probiotic Bifidobacterium animalis ssp.
34855861	6	32	dep	HFD	1147:1149	arg1	5					1144:1144	5	1144:1144	5	1144:1144	In this study C57Bl/6J mice (N = 200) were split in five treatments and daily gavaged: 1) Normal control (NC); 2) HFD; 3) HFD + PDX; 4) HFD + B420 or 5) HFD + PDX + B420 (HFD+S).
34855861	6	32	dep	HFD	1147:1149	arg1	B420					1159:1162	B420	1159:1162	B420	1159:1162	In this study C57Bl/6J mice (N = 200) were split in five treatments and daily gavaged: 1) Normal control (NC); 2) HFD; 3) HFD + PDX; 4) HFD + B420 or 5) HFD + PDX + B420 (HFD+S).
34855861	6	32	dep	HFD	1147:1149	arg1	HFD+S					1165:1169	HFD+S	1165:1169	HFD+S	1165:1169	In this study C57Bl/6J mice (N = 200) were split in five treatments and daily gavaged: 1) Normal control (NC); 2) HFD; 3) HFD + PDX; 4) HFD + B420 or 5) HFD + PDX + B420 (HFD+S).
34855861	6	32	dep	HFD	1147:1149	arg1	PDX					1153:1155	PDX	1153:1155	PDX	1153:1155	In this study C57Bl/6J mice (N = 200) were split in five treatments and daily gavaged: 1) Normal control (NC); 2) HFD; 3) HFD + PDX; 4) HFD + B420 or 5) HFD + PDX + B420 (HFD+S).
34855861	12	33	theme	Akkermansia	1895:1905	arg1	levels					1885:1890	fecal, lleum and colon levels	1862:1890	fecal, lleum and colon levels of Akkermansia	1862:1905	Furthermore, by the inclusion of PDX, fecal, lleum and colon levels of Akkermansia were increased and liver health was improved as the detoxification capacity and levels of methyl-donors were increased.
34855861	2	34	theme	lean	383:386	arg1	phenotype					388:396	the lean phenotype	379:396	the lean phenotype	379:396	The past two decades of research have raised gut microbiota composition as a contributing factor to the development of obesity, and higher abundance of certain bacterial species has been linked to the lean phenotype, such as Akkermansia muciniphila.
34855861	1	35	from	health	127:132	arg1	study					175:179	a multi-compartmental obesogenic mice study	137:179	a multi-compartmental obesogenic mice study	137:179	lactis 420 drives the prevalence of Akkermansia and improves liver health in a multi-compartmental obesogenic mice study.
34855861	5	36	theme	gastrointestinal	911:926	arg1	tract					928:932	the gastrointestinal tract	907:932	the gastrointestinal tract	907:932	lactis 420 (B420), during high fat diet (HFD; 60 kcal% fat) affects microbiota composition in the gastrointestinal tract and adipose tissue, and metabolite levels in gut and liver.
34855861	9	37	theme	rDNA	1503:1506	arg1	sequencing					1508:1517	16S rDNA sequencing	1499:1517	16S rDNA sequencing	1499:1517	The microbiota composition in fecal, ileum, colon and adipose tissue was analyzed using 16S rDNA sequencing, fecal and liver metabolomics were performed by nuclear magnetic resonance (NMR) spectroscopy.
34855861	4	38	theme	fiber	741:745	arg1	polydextrose					748:759	polydextrose	748:759	polydextrose (PDX)	748:765	The aim of this mice study was to determine how administration of a prebiotic fiber, polydextrose (PDX) and a probiotic Bifidobacterium animalis ssp.
34855861	4	38	theme	fiber	741:745	arg1	ssp					808:810	a probiotic Bifidobacterium animalis ssp	771:810	a probiotic Bifidobacterium animalis ssp	771:810	The aim of this mice study was to determine how administration of a prebiotic fiber, polydextrose (PDX) and a probiotic Bifidobacterium animalis ssp.
34855861	4	38	theme	fiber	741:745	arg1	administration					711:724	administration	711:724	administration of a prebiotic fiber	711:745	The aim of this mice study was to determine how administration of a prebiotic fiber, polydextrose (PDX) and a probiotic Bifidobacterium animalis ssp.
34855861	6	39	from	split	1037:1041	arg1	treatments					1051:1060	five treatments	1046:1060	five treatments	1046:1060	In this study C57Bl/6J mice (N = 200) were split in five treatments and daily gavaged: 1) Normal control (NC); 2) HFD; 3) HFD + PDX; 4) HFD + B420 or 5) HFD + PDX + B420 (HFD+S).
34855861	6	39	from	split	1037:1041	arg1	study					1002:1006	this study	997:1006	this study	997:1006	In this study C57Bl/6J mice (N = 200) were split in five treatments and daily gavaged: 1) Normal control (NC); 2) HFD; 3) HFD + PDX; 4) HFD + B420 or 5) HFD + PDX + B420 (HFD+S).
34855861	3	40	theme	pre-	447:450	arg1	ability					436:442	The ability	432:442	The ability of pre- and probiotics to affect metabolic health	432:492	The ability of pre- and probiotics to affect metabolic health could be via microbial community alterations and subsequently changes in metabolite profiles, modulating for example host energy balance via complex signaling pathways.
34855861	5	41	theme	microbiota	881:890	arg1	composition					892:902	microbiota composition	881:902	microbiota composition in the gastrointestinal tract and adipose tissue	881:951	lactis 420 (B420), during high fat diet (HFD; 60 kcal% fat) affects microbiota composition in the gastrointestinal tract and adipose tissue, and metabolite levels in gut and liver.
34855861	4	42	theme	animalis	799:806	arg1	ssp					808:810	a probiotic Bifidobacterium animalis ssp	771:810	a probiotic Bifidobacterium animalis ssp	771:810	The aim of this mice study was to determine how administration of a prebiotic fiber, polydextrose (PDX) and a probiotic Bifidobacterium animalis ssp.
34855861	4	42	theme	animalis	799:806	arg1	administration					711:724	administration	711:724	administration of a prebiotic fiber	711:745	The aim of this mice study was to determine how administration of a prebiotic fiber, polydextrose (PDX) and a probiotic Bifidobacterium animalis ssp.
34855861	0	43	theme	Bifidobacterium	30:44	arg1	ssp					55:57	Bifidobacterium animalis ssp	30:57	Bifidobacterium animalis ssp	30:57	Polydextrose with and without Bifidobacterium animalis ssp.
34855861	3	44	theme	complex	635:641	arg1	pathways					653:660	complex signaling pathways	635:660	complex signaling pathways	635:660	The ability of pre- and probiotics to affect metabolic health could be via microbial community alterations and subsequently changes in metabolite profiles, modulating for example host energy balance via complex signaling pathways.
34855861	2	45	theme	certain	334:340	arg1	species					352:358	certain bacterial species	334:358	certain bacterial species	334:358	The past two decades of research have raised gut microbiota composition as a contributing factor to the development of obesity, and higher abundance of certain bacterial species has been linked to the lean phenotype, such as Akkermansia muciniphila.
34855861	3	46	theme	probiotics	456:465	arg1	ability					436:442	The ability	432:442	The ability of pre- and probiotics to affect metabolic health	432:492	The ability of pre- and probiotics to affect metabolic health could be via microbial community alterations and subsequently changes in metabolite profiles, modulating for example host energy balance via complex signaling pathways.
34855861	8	47	theme	mucosa	1354:1359	arg1	end					1313:1315	end	1313:1315	end of the intervention, ileum and colon mucosa	1313:1359	At end of the intervention, ileum and colon mucosa, adipose tissue and liver samples were collected.
34855861	2	48	attach	linked	369:374	arg1	phenotype					388:396	the lean phenotype	379:396	the lean phenotype	379:396	The past two decades of research have raised gut microbiota composition as a contributing factor to the development of obesity, and higher abundance of certain bacterial species has been linked to the lean phenotype, such as Akkermansia muciniphila.
34855861	2	48	attach	linked	369:374	arg2	abundance					321:329	higher abundance	314:329	higher abundance of certain bacterial species	314:358	The past two decades of research have raised gut microbiota composition as a contributing factor to the development of obesity, and higher abundance of certain bacterial species has been linked to the lean phenotype, such as Akkermansia muciniphila.
34855861	6	49	theme	=	1025:1025	arg1	N					1023:1023	N = 200	1023:1029	N = 200	1023:1029	In this study C57Bl/6J mice (N = 200) were split in five treatments and daily gavaged: 1) Normal control (NC); 2) HFD; 3) HFD + PDX; 4) HFD + B420 or 5) HFD + PDX + B420 (HFD+S).
34855861	6	49	theme	=	1025:1025	arg1	mice					1017:1020	C57Bl/6J mice	1008:1020	C57Bl/6J mice (N = 200)	1008:1030	In this study C57Bl/6J mice (N = 200) were split in five treatments and daily gavaged: 1) Normal control (NC); 2) HFD; 3) HFD + PDX; 4) HFD + B420 or 5) HFD + PDX + B420 (HFD+S).
34855861	4	50	theme	probiotic	773:781	arg1	ssp					808:810	a probiotic Bifidobacterium animalis ssp	771:810	a probiotic Bifidobacterium animalis ssp	771:810	The aim of this mice study was to determine how administration of a prebiotic fiber, polydextrose (PDX) and a probiotic Bifidobacterium animalis ssp.
34855861	4	50	theme	probiotic	773:781	arg1	administration					711:724	administration	711:724	administration of a prebiotic fiber	711:745	The aim of this mice study was to determine how administration of a prebiotic fiber, polydextrose (PDX) and a probiotic Bifidobacterium animalis ssp.
34855861	8	51	theme	adipose	1362:1368	arg1	tissue					1370:1375	adipose tissue	1362:1375	adipose tissue	1362:1375	At end of the intervention, ileum and colon mucosa, adipose tissue and liver samples were collected.
34855861	13	52	theme	new	2033:2035	arg1	results					2037:2043	These new results	2027:2043	These new results	2027:2043	These new results demonstrate how PDX and B420 can affect the interactions between gut, liver and adipose tissue.
34855861	9	53	theme	nuclear	1567:1573	arg1	NMR					1595:1597	NMR	1595:1597	NMR	1595:1597	The microbiota composition in fecal, ileum, colon and adipose tissue was analyzed using 16S rDNA sequencing, fecal and liver metabolomics were performed by nuclear magnetic resonance (NMR) spectroscopy.
34855861	9	53	theme	nuclear	1567:1573	arg1	resonance					1584:1592	nuclear magnetic resonance	1567:1592	nuclear magnetic resonance (NMR) spectroscopy	1567:1611	The microbiota composition in fecal, ileum, colon and adipose tissue was analyzed using 16S rDNA sequencing, fecal and liver metabolomics were performed by nuclear magnetic resonance (NMR) spectroscopy.
34855861	2	54	theme	species	352:358	arg1	abundance					321:329	higher abundance	314:329	higher abundance of certain bacterial species	314:358	The past two decades of research have raised gut microbiota composition as a contributing factor to the development of obesity, and higher abundance of certain bacterial species has been linked to the lean phenotype, such as Akkermansia muciniphila.
34855861	6	55	dep	HFD	1108:1110	arg1	2					1105:1105	2	1105:1105	2	1105:1105	In this study C57Bl/6J mice (N = 200) were split in five treatments and daily gavaged: 1) Normal control (NC); 2) HFD; 3) HFD + PDX; 4) HFD + B420 or 5) HFD + PDX + B420 (HFD+S).
34855861	6	56	theme	C57Bl/6J	1008:1015	arg1	N					1023:1023	N = 200	1023:1029	N = 200	1023:1029	In this study C57Bl/6J mice (N = 200) were split in five treatments and daily gavaged: 1) Normal control (NC); 2) HFD; 3) HFD + PDX; 4) HFD + B420 or 5) HFD + PDX + B420 (HFD+S).
34855861	6	56	theme	C57Bl/6J	1008:1015	arg1	mice					1017:1020	C57Bl/6J mice	1008:1020	C57Bl/6J mice (N = 200)	1008:1030	In this study C57Bl/6J mice (N = 200) were split in five treatments and daily gavaged: 1) Normal control (NC); 2) HFD; 3) HFD + PDX; 4) HFD + B420 or 5) HFD + PDX + B420 (HFD+S).
34855861	9	57	theme	resonance	1584:1592	arg1	spectroscopy					1600:1611	nuclear magnetic resonance (NMR) spectroscopy	1567:1611	nuclear magnetic resonance (NMR) spectroscopy	1567:1611	The microbiota composition in fecal, ileum, colon and adipose tissue was analyzed using 16S rDNA sequencing, fecal and liver metabolomics were performed by nuclear magnetic resonance (NMR) spectroscopy.
34855861	7	58	theme	treatment	1189:1197	arg1	weeks					1180:1184	six weeks	1176:1184	six weeks of treatment	1176:1197	At six weeks of treatment intraperitoneal glucose-tolerance test (IPGTT) was performed, and feces were collected at weeks 0, 3, 6 and 9.
34855861	3	59	theme	microbial	507:515	arg1	alterations					527:537	microbial community alterations	507:537	microbial community alterations	507:537	The ability of pre- and probiotics to affect metabolic health could be via microbial community alterations and subsequently changes in metabolite profiles, modulating for example host energy balance via complex signaling pathways.
34855861	6	60	dep	HFD	1116:1118	arg1	3					1113:1113	3	1113:1113	3	1113:1113	In this study C57Bl/6J mice (N = 200) were split in five treatments and daily gavaged: 1) Normal control (NC); 2) HFD; 3) HFD + PDX; 4) HFD + B420 or 5) HFD + PDX + B420 (HFD+S).
34855861	7	61	theme	intraperitoneal	1199:1213	arg1	test					1233:1236	intraperitoneal glucose-tolerance test	1199:1236	intraperitoneal glucose-tolerance test (IPGTT)	1199:1244	At six weeks of treatment intraperitoneal glucose-tolerance test (IPGTT) was performed, and feces were collected at weeks 0, 3, 6 and 9.
34855861	7	61	theme	intraperitoneal	1199:1213	arg1	IPGTT					1239:1243	IPGTT	1239:1243	IPGTT	1239:1243	At six weeks of treatment intraperitoneal glucose-tolerance test (IPGTT) was performed, and feces were collected at weeks 0, 3, 6 and 9.
34855861	11	62	theme	HFD+S	1811:1815	arg1	group					1817:1821	HFD+S group	1811:1821	HFD+S group	1811:1821	Sequencing the mice adipose tissue (MAT) identified Akkermansia and its prevalence was increased in HFD+S group.
34855861	2	63	theme	obesity	301:307	arg1	development					286:296	the development	282:296	the development of obesity	282:307	The past two decades of research have raised gut microbiota composition as a contributing factor to the development of obesity, and higher abundance of certain bacterial species has been linked to the lean phenotype, such as Akkermansia muciniphila.
34855861	5	64	from	levels	969:974	arg1	tract					928:932	the gastrointestinal tract	907:932	the gastrointestinal tract	907:932	lactis 420 (B420), during high fat diet (HFD; 60 kcal% fat) affects microbiota composition in the gastrointestinal tract and adipose tissue, and metabolite levels in gut and liver.
34855861	5	64	from	levels	969:974	arg1	liver					987:991	liver	987:991	liver	987:991	lactis 420 (B420), during high fat diet (HFD; 60 kcal% fat) affects microbiota composition in the gastrointestinal tract and adipose tissue, and metabolite levels in gut and liver.
34855861	5	64	from	levels	969:974	arg1	tissue					946:951	adipose tissue	938:951	adipose tissue	938:951	lactis 420 (B420), during high fat diet (HFD; 60 kcal% fat) affects microbiota composition in the gastrointestinal tract and adipose tissue, and metabolite levels in gut and liver.
34855861	5	64	from	levels	969:974	arg1	gut					979:981	gut	979:981	gut	979:981	lactis 420 (B420), during high fat diet (HFD; 60 kcal% fat) affects microbiota composition in the gastrointestinal tract and adipose tissue, and metabolite levels in gut and liver.
34855861	11	65	theme	mice	1726:1729	arg1	MAT					1747:1749	MAT	1747:1749	MAT	1747:1749	Sequencing the mice adipose tissue (MAT) identified Akkermansia and its prevalence was increased in HFD+S group.
34855861	11	65	theme	mice	1726:1729	arg1	tissue					1739:1744	the mice adipose tissue	1722:1744	Sequencing the mice adipose tissue (MAT)	1711:1750	Sequencing the mice adipose tissue (MAT) identified Akkermansia and its prevalence was increased in HFD+S group.
34855861	10	66	theme	HFD+PDX	1632:1638	arg1	intervention					1640:1651	HFD+PDX intervention	1632:1651	HFD+PDX intervention	1632:1651	It was found that HFD+PDX intervention reduced body weight gain and hepatic fat compared to HFD.
34855861	2	67	theme	microbiota	231:240	arg1	factor					272:277	a contributing factor	257:277	a contributing factor to the development of obesity	257:307	The past two decades of research have raised gut microbiota composition as a contributing factor to the development of obesity, and higher abundance of certain bacterial species has been linked to the lean phenotype, such as Akkermansia muciniphila.
34855861	2	67	theme	microbiota	231:240	arg1	composition					242:252	gut microbiota composition	227:252	gut microbiota composition	227:252	The past two decades of research have raised gut microbiota composition as a contributing factor to the development of obesity, and higher abundance of certain bacterial species has been linked to the lean phenotype, such as Akkermansia muciniphila.
34855861	6	68	dep	HFD	1130:1132	arg1	4					1127:1127	4	1127:1127	4	1127:1127	In this study C57Bl/6J mice (N = 200) were split in five treatments and daily gavaged: 1) Normal control (NC); 2) HFD; 3) HFD + PDX; 4) HFD + B420 or 5) HFD + PDX + B420 (HFD+S).
34855861	9	69	theme	microbiota	1415:1424	arg1	composition					1426:1436	The microbiota composition	1411:1436	The microbiota composition in fecal, ileum, colon and adipose tissue	1411:1478	The microbiota composition in fecal, ileum, colon and adipose tissue was analyzed using 16S rDNA sequencing, fecal and liver metabolomics were performed by nuclear magnetic resonance (NMR) spectroscopy.
34855861	12	70	theme	detoxification	1959:1972	arg1	capacity					1974:1981	the detoxification capacity	1955:1981	the detoxification capacity	1955:1981	Furthermore, by the inclusion of PDX, fecal, lleum and colon levels of Akkermansia were increased and liver health was improved as the detoxification capacity and levels of methyl-donors were increased.
34855861	12	70	theme	detoxification	1959:1972	arg1	health					1932:1937	liver health	1926:1937	liver health	1926:1937	Furthermore, by the inclusion of PDX, fecal, lleum and colon levels of Akkermansia were increased and liver health was improved as the detoxification capacity and levels of methyl-donors were increased.
34855861	10	71	theme	weight	1666:1671	arg1	gain					1673:1676	body weight gain	1661:1676	body weight gain	1661:1676	It was found that HFD+PDX intervention reduced body weight gain and hepatic fat compared to HFD.
34855861	3	72	theme	energy	616:621	arg1	balance					623:629	host energy balance	611:629	host energy balance	611:629	The ability of pre- and probiotics to affect metabolic health could be via microbial community alterations and subsequently changes in metabolite profiles, modulating for example host energy balance via complex signaling pathways.
34855861	5	73	theme	kcal	862:865	arg1	fat					868:870	60 kcal% fat	859:870	HFD; 60 kcal% fat	854:870	lactis 420 (B420), during high fat diet (HFD; 60 kcal% fat) affects microbiota composition in the gastrointestinal tract and adipose tissue, and metabolite levels in gut and liver.
34855861	6	74	theme	Normal	1084:1089	arg1	NC					1100:1101	NC	1100:1101	NC	1100:1101	In this study C57Bl/6J mice (N = 200) were split in five treatments and daily gavaged: 1) Normal control (NC); 2) HFD; 3) HFD + PDX; 4) HFD + B420 or 5) HFD + PDX + B420 (HFD+S).
34855861	6	74	theme	Normal	1084:1089	arg1	control					1091:1097	Normal control	1084:1097	1) Normal control (NC); 2) HFD; 3) HFD + PDX; 4) HFD + B420 or 5) HFD + PDX + B420 (HFD+S)	1081:1170	In this study C57Bl/6J mice (N = 200) were split in five treatments and daily gavaged: 1) Normal control (NC); 2) HFD; 3) HFD + PDX; 4) HFD + B420 or 5) HFD + PDX + B420 (HFD+S).
34855861	5	75	theme	fat	844:846	arg1	diet					848:851	high fat diet	839:851	high fat diet (HFD; 60 kcal% fat)	839:871	lactis 420 (B420), during high fat diet (HFD; 60 kcal% fat) affects microbiota composition in the gastrointestinal tract and adipose tissue, and metabolite levels in gut and liver.
34855861	9	76	from	composition	1426:1436	arg1	colon					1455:1459	colon	1455:1459	colon	1455:1459	The microbiota composition in fecal, ileum, colon and adipose tissue was analyzed using 16S rDNA sequencing, fecal and liver metabolomics were performed by nuclear magnetic resonance (NMR) spectroscopy.
34855861	9	76	from	composition	1426:1436	arg1	tissue					1473:1478	adipose tissue	1465:1478	adipose tissue	1465:1478	The microbiota composition in fecal, ileum, colon and adipose tissue was analyzed using 16S rDNA sequencing, fecal and liver metabolomics were performed by nuclear magnetic resonance (NMR) spectroscopy.
34855861	9	76	from	composition	1426:1436	arg1	ileum					1448:1452	ileum	1448:1452	ileum	1448:1452	The microbiota composition in fecal, ileum, colon and adipose tissue was analyzed using 16S rDNA sequencing, fecal and liver metabolomics were performed by nuclear magnetic resonance (NMR) spectroscopy.
34855861	9	76	from	composition	1426:1436	arg1	fecal					1441:1445	fecal	1441:1445	fecal	1441:1445	The microbiota composition in fecal, ileum, colon and adipose tissue was analyzed using 16S rDNA sequencing, fecal and liver metabolomics were performed by nuclear magnetic resonance (NMR) spectroscopy.
34855861	1	77	theme	obesogenic	159:168	arg1	mice					170:173	multi-compartmental obesogenic mice	139:173	a multi-compartmental obesogenic mice study	137:179	lactis 420 drives the prevalence of Akkermansia and improves liver health in a multi-compartmental obesogenic mice study.
34855861	4	78	theme	study	684:688	arg1	aim					667:669	The aim	663:669	The aim of this mice study	663:688	The aim of this mice study was to determine how administration of a prebiotic fiber, polydextrose (PDX) and a probiotic Bifidobacterium animalis ssp.
34855861	3	79	from	alterations	527:537	arg1	profiles					578:585	metabolite profiles	567:585	metabolite profiles	567:585	The ability of pre- and probiotics to affect metabolic health could be via microbial community alterations and subsequently changes in metabolite profiles, modulating for example host energy balance via complex signaling pathways.
34855861	12	80	theme	colon	1879:1883	arg1	levels					1885:1890	fecal, lleum and colon levels	1862:1890	fecal, lleum and colon levels of Akkermansia	1862:1905	Furthermore, by the inclusion of PDX, fecal, lleum and colon levels of Akkermansia were increased and liver health was improved as the detoxification capacity and levels of methyl-donors were increased.
34855861	12	81	theme	liver	1926:1930	arg1	capacity					1974:1981	the detoxification capacity	1955:1981	the detoxification capacity	1955:1981	Furthermore, by the inclusion of PDX, fecal, lleum and colon levels of Akkermansia were increased and liver health was improved as the detoxification capacity and levels of methyl-donors were increased.
34855861	12	81	theme	liver	1926:1930	arg1	health					1932:1937	liver health	1926:1937	liver health	1926:1937	Furthermore, by the inclusion of PDX, fecal, lleum and colon levels of Akkermansia were increased and liver health was improved as the detoxification capacity and levels of methyl-donors were increased.
34855861	9	82	theme	16S	1499:1501	arg1	sequencing					1508:1517	16S rDNA sequencing	1499:1517	16S rDNA sequencing	1499:1517	The microbiota composition in fecal, ileum, colon and adipose tissue was analyzed using 16S rDNA sequencing, fecal and liver metabolomics were performed by nuclear magnetic resonance (NMR) spectroscopy.
34855861	5	83	dep	HFD	854:856	arg1	fat					868:870	60 kcal% fat	859:870	HFD; 60 kcal% fat	854:870	lactis 420 (B420), during high fat diet (HFD; 60 kcal% fat) affects microbiota composition in the gastrointestinal tract and adipose tissue, and metabolite levels in gut and liver.
34855861	4	84	dep	determine	697:705	arg1	polydextrose					748:759	polydextrose	748:759	polydextrose (PDX)	748:765	The aim of this mice study was to determine how administration of a prebiotic fiber, polydextrose (PDX) and a probiotic Bifidobacterium animalis ssp.
34855861	4	84	dep	determine	697:705	arg1	ssp					808:810	a probiotic Bifidobacterium animalis ssp	771:810	a probiotic Bifidobacterium animalis ssp	771:810	The aim of this mice study was to determine how administration of a prebiotic fiber, polydextrose (PDX) and a probiotic Bifidobacterium animalis ssp.
34855861	4	84	dep	determine	697:705	arg1	administration					711:724	administration	711:724	administration of a prebiotic fiber	711:745	The aim of this mice study was to determine how administration of a prebiotic fiber, polydextrose (PDX) and a probiotic Bifidobacterium animalis ssp.
34855861	2	85	theme	Akkermansia	407:417	arg1	muciniphila					419:429	Akkermansia muciniphila	407:429	Akkermansia muciniphila	407:429	The past two decades of research have raised gut microbiota composition as a contributing factor to the development of obesity, and higher abundance of certain bacterial species has been linked to the lean phenotype, such as Akkermansia muciniphila.
34855861	3	86	theme	signaling	643:651	arg1	pathways					653:660	complex signaling pathways	635:660	complex signaling pathways	635:660	The ability of pre- and probiotics to affect metabolic health could be via microbial community alterations and subsequently changes in metabolite profiles, modulating for example host energy balance via complex signaling pathways.
34855861	5	87	dep	diet	848:851	arg1	HFD					854:856	HFD	854:856	HFD; 60 kcal% fat	854:870	lactis 420 (B420), during high fat diet (HFD; 60 kcal% fat) affects microbiota composition in the gastrointestinal tract and adipose tissue, and metabolite levels in gut and liver.
34855861	6	88	from	treatments	1051:1060	arg1	split					1037:1041	split	1037:1041	split	1037:1041	In this study C57Bl/6J mice (N = 200) were split in five treatments and daily gavaged: 1) Normal control (NC); 2) HFD; 3) HFD + PDX; 4) HFD + B420 or 5) HFD + PDX + B420 (HFD+S).
34855861	0	89	theme	animalis	46:53	arg1	ssp					55:57	Bifidobacterium animalis ssp	30:57	Bifidobacterium animalis ssp	30:57	Polydextrose with and without Bifidobacterium animalis ssp.
34855861	12	90	theme	lleum	1869:1873	arg1	levels					1885:1890	fecal, lleum and colon levels	1862:1890	fecal, lleum and colon levels of Akkermansia	1862:1905	Furthermore, by the inclusion of PDX, fecal, lleum and colon levels of Akkermansia were increased and liver health was improved as the detoxification capacity and levels of methyl-donors were increased.
34855861	4	91	theme	Bifidobacterium	783:797	arg1	ssp					808:810	a probiotic Bifidobacterium animalis ssp	771:810	a probiotic Bifidobacterium animalis ssp	771:810	The aim of this mice study was to determine how administration of a prebiotic fiber, polydextrose (PDX) and a probiotic Bifidobacterium animalis ssp.
34855861	4	91	theme	Bifidobacterium	783:797	arg1	administration					711:724	administration	711:724	administration of a prebiotic fiber	711:745	The aim of this mice study was to determine how administration of a prebiotic fiber, polydextrose (PDX) and a probiotic Bifidobacterium animalis ssp.
34855861	5	92	theme	metabolite	958:967	arg1	levels					969:974	metabolite levels	958:974	metabolite levels in gut and liver	958:991	lactis 420 (B420), during high fat diet (HFD; 60 kcal% fat) affects microbiota composition in the gastrointestinal tract and adipose tissue, and metabolite levels in gut and liver.
34855861	2	93	theme	research	206:213	arg1	decades					195:201	The past two decades	182:201	The past two decades of research	182:213	The past two decades of research have raised gut microbiota composition as a contributing factor to the development of obesity, and higher abundance of certain bacterial species has been linked to the lean phenotype, such as Akkermansia muciniphila.
34855861	12	94	theme	PDX	1857:1859	arg1	inclusion					1844:1852	the inclusion	1840:1852	the inclusion of PDX	1840:1859	Furthermore, by the inclusion of PDX, fecal, lleum and colon levels of Akkermansia were increased and liver health was improved as the detoxification capacity and levels of methyl-donors were increased.
34855861	3	95	theme	metabolic	477:485	arg1	health					487:492	metabolic health	477:492	metabolic health	477:492	The ability of pre- and probiotics to affect metabolic health could be via microbial community alterations and subsequently changes in metabolite profiles, modulating for example host energy balance via complex signaling pathways.
34855861	2	96	theme	higher	314:319	arg1	abundance					321:329	higher abundance	314:329	higher abundance of certain bacterial species	314:358	The past two decades of research have raised gut microbiota composition as a contributing factor to the development of obesity, and higher abundance of certain bacterial species has been linked to the lean phenotype, such as Akkermansia muciniphila.
34855861	5	97	theme	adipose	938:944	arg1	tissue					946:951	adipose tissue	938:951	adipose tissue	938:951	lactis 420 (B420), during high fat diet (HFD; 60 kcal% fat) affects microbiota composition in the gastrointestinal tract and adipose tissue, and metabolite levels in gut and liver.
34855861	8	98	theme	intervention	1324:1335	arg1	end					1313:1315	end	1313:1315	end of the intervention, ileum and colon mucosa	1313:1359	At end of the intervention, ileum and colon mucosa, adipose tissue and liver samples were collected.
34855861	8	99	theme	liver	1381:1385	arg1	samples					1387:1393	liver samples	1381:1393	liver samples	1381:1393	At end of the intervention, ileum and colon mucosa, adipose tissue and liver samples were collected.
34018615	4	0	theme	inhibition	993:1002	arg1	activities					1032:1041	good glycosidase inhibition and free radical scavenging activities	976:1041	good glycosidase inhibition and free radical scavenging activities	976:1041	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	0	1	theme	typical	167:173	arg1	teas					188:191	four typical Chinese dark teas	162:191	four typical Chinese dark teas	162:191	Comparative evaluation for phytochemical composition and regulation of blood glucose, hepatic oxidative stress and insulin resistance in mice and HepG2 models of four typical Chinese dark teas.
34018615	4	2	theme	free	1008:1011	arg1	scavenging					1021:1030	free radical scavenging	1008:1030	free radical scavenging	1008:1030	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	6	3	with	products	2020:2027	arg1	effects					2048:2054	anti-diabetic effects	2034:2054	anti-diabetic effects	2034:2054	CONCLUSION Dark tea is a highly attractive candidate for developing antidiabetic food, LBT and PET may be good natural sources of agricultural products with anti-diabetic effects.
34018615	5	4	theme	hypoglycemic	1550:1561	arg1	activity					1563:1570	in vivo hypoglycemic activity	1542:1570	in vivo hypoglycemic activity	1542:1570	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	4	5	theme	related	1320:1326	arg1	activities					1309:1318	the key enzyme activities	1294:1318	the key enzyme activities related to glucose metabolism and antioxidant activity	1294:1373	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	0	6	theme	dark	183:186	arg1	teas					188:191	four typical Chinese dark teas	162:191	four typical Chinese dark teas	162:191	Comparative evaluation for phytochemical composition and regulation of blood glucose, hepatic oxidative stress and insulin resistance in mice and HepG2 models of four typical Chinese dark teas.
34018615	5	7	theme	brick	1455:1459	arg1	LBT					1466:1468	LBT	1466:1468	LBT	1466:1468	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	5	7	theme	brick	1455:1459	arg1	tea					1461:1463	Liubao brick tea	1448:1463	Liubao brick tea (LBT)	1448:1469	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	1	8	theme	teas	247:250	arg1	one					226:228	one	226:228	one	226:228	BACKGROUND Dark tea, comprising one of the six major teas, has many biological activities, which originate from their active substrates, such as polyphenols, polysaccharides, and so on.
34018615	1	8	theme	teas	247:250	arg1	teas					247:250	the six major teas	233:250	the six major teas	233:250	BACKGROUND Dark tea, comprising one of the six major teas, has many biological activities, which originate from their active substrates, such as polyphenols, polysaccharides, and so on.
34018615	5	9	theme	brick	1632:1636	arg1	tea					1638:1640	Qingzhuan brick tea	1622:1640	Qingzhuan brick tea	1622:1640	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	4	10	theme	glucose	1331:1337	arg1	metabolism					1339:1348	glucose metabolism	1331:1348	glucose metabolism	1331:1348	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	3	11	dep	RESULTS	541:547	arg1	analyzed					585:592	analyzed	585:592	analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts	585:844	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	4	12	theme	insulin	1093:1099	arg1	resistance					1101:1110	insulin resistance	1093:1110	insulin resistance	1093:1110	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	6	13	theme	Dark	1888:1891	arg1	candidate					1920:1928	a highly attractive candidate	1900:1928	a highly attractive candidate for developing antidiabetic food, LBT	1900:1966	CONCLUSION Dark tea is a highly attractive candidate for developing antidiabetic food, LBT and PET may be good natural sources of agricultural products with anti-diabetic effects.
34018615	6	13	theme	Dark	1888:1891	arg1	tea					1893:1895	CONCLUSION Dark tea	1877:1895	CONCLUSION Dark tea	1877:1895	CONCLUSION Dark tea is a highly attractive candidate for developing antidiabetic food, LBT and PET may be good natural sources of agricultural products with anti-diabetic effects.
34018615	3	14	theme	type	717:720	arg1	mellitus					733:740	type 2 diabetes mellitus	717:740	type 2 diabetes mellitus mice	717:745	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	5	15	contain	had	1664:1666	arg1	tea					1660:1662	Fuzhuan brick tea	1646:1662	Fuzhuan brick tea	1646:1662	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	5	15	contain	had	1664:1666	arg2	activity					1699:1706	better free radical scavenging activity	1668:1706	better free radical scavenging activity	1668:1706	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	5	15	contain	had	1664:1666	arg1	tea					1638:1640	Qingzhuan brick tea	1622:1640	Qingzhuan brick tea	1622:1640	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	4	16	contain	had	972:974	arg2	activities					1032:1041	good glycosidase inhibition and free radical scavenging activities	976:1041	good glycosidase inhibition and free radical scavenging activities	976:1041	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	4	16	contain	had	972:974	arg1	they					963:966	they	963:966	they	963:966	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	4	16	contain	had	972:974	arg2	alleviation					1078:1088	alleviation	1078:1088	alleviation of insulin resistance	1078:1110	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	4	16	contain	had	972:974	arg2	activity					1065:1072	in vivo hypoglycemic activity	1044:1072	in vivo hypoglycemic activity	1044:1072	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	3	17	from	cells	806:810	arg1	activities					676:685	free radical scavenging activities	652:685	free radical scavenging activities	652:685	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	3	17	from	cells	806:810	arg1	activity					705:712	hypoglycemic activity	692:712	hypoglycemic activity in type 2 diabetes mellitus mice	692:745	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	3	17	from	cells	806:810	arg1	alleviation					763:773	the alleviation	759:773	the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts	759:844	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	3	17	from	cells	806:810	arg1	composition					612:622	the phytochemical composition	594:622	the phytochemical composition	594:622	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	3	17	from	cells	806:810	arg1	inhibition					637:646	glycosidase inhibition	625:646	glycosidase inhibition	625:646	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	5	18	theme	brick	1654:1658	arg1	tea					1660:1662	Fuzhuan brick tea	1646:1662	Fuzhuan brick tea	1646:1662	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	4	19	theme	factor	1421:1426	arg1	levels					1428:1433	inflammatory factor levels	1408:1433	inflammatory factor levels	1408:1433	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	0	20	theme	resistance	123:132	arg1	regulation					57:66	regulation	57:66	regulation	57:66	Comparative evaluation for phytochemical composition and regulation of blood glucose, hepatic oxidative stress and insulin resistance in mice and HepG2 models of four typical Chinese dark teas.
34018615	0	20	theme	resistance	123:132	arg1	composition					41:51	phytochemical composition	27:51	phytochemical composition	27:51	Comparative evaluation for phytochemical composition and regulation of blood glucose, hepatic oxidative stress and insulin resistance in mice and HepG2 models of four typical Chinese dark teas.
34018615	1	21	theme	BACKGROUND	194:203	arg1	tea					210:212	BACKGROUND Dark tea	194:212	BACKGROUND Dark tea	194:212	BACKGROUND Dark tea, comprising one of the six major teas, has many biological activities, which originate from their active substrates, such as polyphenols, polysaccharides, and so on.
34018615	4	22	theme	phytochemical	875:887	arg1	composition					889:899	the phytochemical composition	871:899	the phytochemical composition of dark tea aqueous extracts	871:928	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	4	22	theme	phytochemical	875:887	arg1	different					948:956	different	948:956	different	948:956	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	6	23	theme	food	1958:1961	arg1	LBT					1964:1966	antidiabetic food, LBT	1945:1966	LBT	1964:1966	CONCLUSION Dark tea is a highly attractive candidate for developing antidiabetic food, LBT and PET may be good natural sources of agricultural products with anti-diabetic effects.
34018615	4	24	theme	antioxidant	1354:1364	arg1	activity					1366:1373	antioxidant activity	1354:1373	antioxidant activity	1354:1373	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	3	25	theme	diabetes	724:731	arg1	mellitus					733:740	type 2 diabetes mellitus	717:740	type 2 diabetes mellitus mice	717:745	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	0	26	theme	HepG2	146:150	arg1	models					152:157	HepG2 models	146:157	HepG2 models of four typical Chinese dark teas	146:191	Comparative evaluation for phytochemical composition and regulation of blood glucose, hepatic oxidative stress and insulin resistance in mice and HepG2 models of four typical Chinese dark teas.
34018615	4	27	theme	phosphatidylinositol	1141:1160	arg1	pathway					1237:1243	the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway	1137:1243	the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels	1137:1433	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	7	28	theme	Chemical	2075:2082	arg1	Industry					2084:2091	Chemical Industry	2075:2091	Chemical Industry	2075:2091	© 2021 Society of Chemical Industry.
34018615	4	29	theme	extracts	921:928	arg1	composition					889:899	the phytochemical composition	871:899	the phytochemical composition of dark tea aqueous extracts	871:928	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	4	29	theme	extracts	921:928	arg1	different					948:956	different	948:956	different	948:956	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	0	30	theme	blood	71:75	arg1	glucose					77:83	blood glucose	71:83	blood glucose	71:83	Comparative evaluation for phytochemical composition and regulation of blood glucose, hepatic oxidative stress and insulin resistance in mice and HepG2 models of four typical Chinese dark teas.
34018615	4	31	theme	good	976:979	arg1	inhibition					993:1002	good glycosidase inhibition	976:1002	good glycosidase inhibition	976:1002	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	3	32	theme	tea	825:827	arg1	extracts					837:844	four dark tea aqueous extracts	815:844	four dark tea aqueous extracts	815:844	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	5	33	contain	possessed	1492:1500	arg1	LBT					1466:1468	LBT	1466:1468	LBT	1466:1468	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	5	33	contain	possessed	1492:1500	arg2	activity					1532:1539	better glycosidase inhibitory activity	1502:1539	better glycosidase inhibitory activity	1502:1539	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	5	33	contain	possessed	1492:1500	arg2	activity					1563:1570	in vivo hypoglycemic activity	1542:1570	in vivo hypoglycemic activity	1542:1570	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	5	33	contain	possessed	1492:1500	arg1	tea					1461:1463	Liubao brick tea	1448:1463	Liubao brick tea (LBT)	1448:1469	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	5	33	contain	possessed	1492:1500	arg2	activity					1604:1611	improved insulin resistance activity	1576:1611	improved insulin resistance activity	1576:1611	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	5	33	contain	possessed	1492:1500	arg1	tea					1482:1484	Pu-erh tea	1475:1484	Pu-erh tea (PET)	1475:1490	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	5	33	contain	possessed	1492:1500	arg1	PET					1487:1489	PET	1487:1489	PET	1487:1489	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	5	34	theme	Pu-erh	1475:1480	arg1	tea					1482:1484	Pu-erh tea	1475:1484	Pu-erh tea (PET)	1475:1490	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	5	34	theme	Pu-erh	1475:1480	arg1	PET					1487:1489	PET	1487:1489	PET	1487:1489	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	3	35	theme	insulin	778:784	arg1	resistance					786:795	insulin resistance	778:795	insulin resistance in HepG2 cells of four dark tea aqueous extracts	778:844	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	1	36	theme	active	312:317	arg1	substrates					319:328	their active substrates	306:328	their active substrates	306:328	BACKGROUND Dark tea, comprising one of the six major teas, has many biological activities, which originate from their active substrates, such as polyphenols, polysaccharides, and so on.
34018615	1	36	theme	active	312:317	arg1	so					373:374	so	373:374	so	373:374	BACKGROUND Dark tea, comprising one of the six major teas, has many biological activities, which originate from their active substrates, such as polyphenols, polysaccharides, and so on.
34018615	1	36	theme	active	312:317	arg1	polyphenols					339:349	polyphenols	339:349	polyphenols	339:349	BACKGROUND Dark tea, comprising one of the six major teas, has many biological activities, which originate from their active substrates, such as polyphenols, polysaccharides, and so on.
34018615	6	37	theme	natural	1988:1994	arg1	sources					1996:2002	good natural sources	1983:2002	good natural sources of agricultural products with anti-diabetic effects	1983:2054	CONCLUSION Dark tea is a highly attractive candidate for developing antidiabetic food, LBT and PET may be good natural sources of agricultural products with anti-diabetic effects.
34018615	0	38	theme	oxidative	94:102	arg1	stress					104:109	hepatic oxidative stress	86:109	hepatic oxidative stress	86:109	Comparative evaluation for phytochemical composition and regulation of blood glucose, hepatic oxidative stress and insulin resistance in mice and HepG2 models of four typical Chinese dark teas.
34018615	5	39	theme	inhibitory	1521:1530	arg1	activity					1532:1539	better glycosidase inhibitory activity	1502:1539	better glycosidase inhibitory activity	1502:1539	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	4	40	theme	tea	909:911	arg1	extracts					921:928	dark tea aqueous extracts	904:928	dark tea aqueous extracts	904:928	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	0	41	from	regulation	57:66	arg1	mice					137:140	mice	137:140	mice	137:140	Comparative evaluation for phytochemical composition and regulation of blood glucose, hepatic oxidative stress and insulin resistance in mice and HepG2 models of four typical Chinese dark teas.
34018615	0	41	from	regulation	57:66	arg1	models					152:157	HepG2 models	146:157	HepG2 models of four typical Chinese dark teas	146:191	Comparative evaluation for phytochemical composition and regulation of blood glucose, hepatic oxidative stress and insulin resistance in mice and HepG2 models of four typical Chinese dark teas.
34018615	3	42	from	activity	705:712	arg1	cells					806:810	HepG2 cells	800:810	HepG2 cells of four dark tea aqueous extracts	800:844	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	3	42	from	activity	705:712	arg1	mice					742:745	type 2 diabetes mellitus mice	717:745	type 2 diabetes mellitus mice	717:745	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	5	43	theme	radical	1680:1686	arg1	activity					1699:1706	better free radical scavenging activity	1668:1706	better free radical scavenging activity	1668:1706	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	4	44	dep	in	1044:1045	arg1	vivo					1047:1050	vivo	1047:1050	vivo	1047:1050	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	3	45	theme	extracts	837:844	arg1	cells					806:810	HepG2 cells	800:810	HepG2 cells of four dark tea aqueous extracts	800:844	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	0	46	from	composition	41:51	arg1	mice					137:140	mice	137:140	mice	137:140	Comparative evaluation for phytochemical composition and regulation of blood glucose, hepatic oxidative stress and insulin resistance in mice and HepG2 models of four typical Chinese dark teas.
34018615	0	46	from	composition	41:51	arg1	models					152:157	HepG2 models	146:157	HepG2 models of four typical Chinese dark teas	146:191	Comparative evaluation for phytochemical composition and regulation of blood glucose, hepatic oxidative stress and insulin resistance in mice and HepG2 models of four typical Chinese dark teas.
34018615	4	47	theme	proliferation-activated	1186:1208	arg1	pathway					1237:1243	the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway	1137:1243	the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels	1137:1433	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	0	48	theme	Comparative	0:10	arg1	evaluation					12:21	Comparative evaluation	0:21	Comparative evaluation for phytochemical composition and regulation of blood glucose, hepatic oxidative stress and insulin resistance in mice and HepG2 models of four typical Chinese dark teas.	0:192	Comparative evaluation for phytochemical composition and regulation of blood glucose, hepatic oxidative stress and insulin resistance in mice and HepG2 models of four typical Chinese dark teas.
34018615	4	49	theme	glycosidase	981:991	arg1	inhibition					993:1002	good glycosidase inhibition	976:1002	good glycosidase inhibition	976:1002	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	3	50	from	alleviation	763:773	arg1	cells					806:810	HepG2 cells	800:810	HepG2 cells of four dark tea aqueous extracts	800:844	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	3	50	from	alleviation	763:773	arg1	mice					742:745	type 2 diabetes mellitus mice	717:745	type 2 diabetes mellitus mice	717:745	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	4	51	theme	cascade	1219:1225	arg1	pathway					1237:1243	the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway	1137:1243	the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels	1137:1433	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	1	52	theme	many	257:260	arg1	activities					273:282	many biological activities	257:282	many biological activities	257:282	BACKGROUND Dark tea, comprising one of the six major teas, has many biological activities, which originate from their active substrates, such as polyphenols, polysaccharides, and so on.
34018615	2	53	theme	prominent	423:431	arg1	activities					433:442	its most prominent activities	414:442	its most prominent activities	414:442	The hypoglycemic effect is one of its most prominent activities, although less is known about their evaluation and potential role in the hypoglycemic mechanism.
34018615	3	54	theme	phytochemical	598:610	arg1	activities					676:685	free radical scavenging activities	652:685	free radical scavenging activities	652:685	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	3	54	theme	phytochemical	598:610	arg1	composition					612:622	the phytochemical composition	594:622	the phytochemical composition	594:622	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	3	54	theme	phytochemical	598:610	arg1	inhibition					637:646	glycosidase inhibition	625:646	glycosidase inhibition	625:646	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	5	55	theme	better	1668:1673	arg1	activity					1699:1706	better free radical scavenging activity	1668:1706	better free radical scavenging activity	1668:1706	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	4	56	theme	key	1298:1300	arg1	activities					1309:1318	the key enzyme activities	1294:1318	the key enzyme activities related to glucose metabolism and antioxidant activity	1294:1373	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	6	57	theme	anti-diabetic	2034:2046	arg1	effects					2048:2054	anti-diabetic effects	2034:2054	anti-diabetic effects	2034:2054	CONCLUSION Dark tea is a highly attractive candidate for developing antidiabetic food, LBT and PET may be good natural sources of agricultural products with anti-diabetic effects.
34018615	5	58	theme	in	1542:1543	arg1	activity					1563:1570	in vivo hypoglycemic activity	1542:1570	in vivo hypoglycemic activity	1542:1570	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	5	59	theme	better	1502:1507	arg1	activity					1532:1539	better glycosidase inhibitory activity	1502:1539	better glycosidase inhibitory activity	1502:1539	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	6	60	theme	products	2020:2027	arg1	sources					1996:2002	good natural sources	1983:2002	good natural sources of agricultural products with anti-diabetic effects	1983:2054	CONCLUSION Dark tea is a highly attractive candidate for developing antidiabetic food, LBT and PET may be good natural sources of agricultural products with anti-diabetic effects.
34018615	6	60	theme	products	2020:2027	arg1	candidate					1920:1928	a highly attractive candidate	1900:1928	a highly attractive candidate for developing antidiabetic food, LBT	1900:1966	CONCLUSION Dark tea is a highly attractive candidate for developing antidiabetic food, LBT and PET may be good natural sources of agricultural products with anti-diabetic effects.
34018615	6	60	theme	products	2020:2027	arg1	tea					1893:1895	CONCLUSION Dark tea	1877:1895	CONCLUSION Dark tea	1877:1895	CONCLUSION Dark tea is a highly attractive candidate for developing antidiabetic food, LBT and PET may be good natural sources of agricultural products with anti-diabetic effects.
34018615	5	61	theme	distinct	1741:1748	arg1	catechins					1830:1838	catechins	1830:1838	catechins	1830:1838	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	5	61	theme	distinct	1741:1748	arg1	elements					1867:1874	some elements	1862:1874	some elements	1862:1874	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	5	61	theme	distinct	1741:1748	arg1	proteins					1790:1797	tea proteins	1786:1797	tea proteins	1786:1797	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	5	61	theme	distinct	1741:1748	arg1	polysaccharides					1800:1814	polysaccharides	1800:1814	polysaccharides	1800:1814	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	5	61	theme	distinct	1741:1748	arg1	compositions					1764:1775	their distinct phytochemical compositions	1735:1775	their distinct phytochemical compositions	1735:1775	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	5	61	theme	distinct	1741:1748	arg1	pigments					1849:1856	tea pigments	1845:1856	tea pigments	1845:1856	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	5	61	theme	distinct	1741:1748	arg1	polyphenols					1817:1827	polyphenols	1817:1827	polyphenols	1817:1827	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	4	62	theme	in	1044:1045	arg1	activity					1065:1072	in vivo hypoglycemic activity	1044:1072	in vivo hypoglycemic activity	1044:1072	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	2	63	from	evaluation	480:489	arg1	mechanism					530:538	the hypoglycemic mechanism	513:538	the hypoglycemic mechanism	513:538	The hypoglycemic effect is one of its most prominent activities, although less is known about their evaluation and potential role in the hypoglycemic mechanism.
34018615	3	64	from	composition	612:622	arg1	cells					806:810	HepG2 cells	800:810	HepG2 cells of four dark tea aqueous extracts	800:844	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	3	64	from	composition	612:622	arg1	mice					742:745	type 2 diabetes mellitus mice	717:745	type 2 diabetes mellitus mice	717:745	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	5	65	dep	in	1542:1543	arg1	vivo					1545:1548	vivo	1545:1548	vivo	1545:1548	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	5	66	theme	resistance	1593:1602	arg1	activity					1604:1611	improved insulin resistance activity	1576:1611	improved insulin resistance activity	1576:1611	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	3	67	theme	scavenging	665:674	arg1	composition					612:622	the phytochemical composition	594:622	the phytochemical composition	594:622	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	3	67	theme	scavenging	665:674	arg1	activities					676:685	free radical scavenging activities	652:685	free radical scavenging activities	652:685	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	0	68	theme	Chinese	175:181	arg1	teas					188:191	four typical Chinese dark teas	162:191	four typical Chinese dark teas	162:191	Comparative evaluation for phytochemical composition and regulation of blood glucose, hepatic oxidative stress and insulin resistance in mice and HepG2 models of four typical Chinese dark teas.
34018615	4	69	theme	radical	1013:1019	arg1	scavenging					1021:1030	free radical scavenging	1008:1030	free radical scavenging	1008:1030	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	3	70	theme	free	652:655	arg1	scavenging					665:674	free radical scavenging	652:674	free radical scavenging activities	652:685	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	0	71	theme	teas	188:191	arg1	mice					137:140	mice	137:140	mice	137:140	Comparative evaluation for phytochemical composition and regulation of blood glucose, hepatic oxidative stress and insulin resistance in mice and HepG2 models of four typical Chinese dark teas.
34018615	0	71	theme	teas	188:191	arg1	models					152:157	HepG2 models	146:157	HepG2 models of four typical Chinese dark teas	146:191	Comparative evaluation for phytochemical composition and regulation of blood glucose, hepatic oxidative stress and insulin resistance in mice and HepG2 models of four typical Chinese dark teas.
34018615	5	72	theme	Liubao	1448:1453	arg1	LBT					1466:1468	LBT	1466:1468	LBT	1466:1468	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	5	72	theme	Liubao	1448:1453	arg1	tea					1461:1463	Liubao brick tea	1448:1463	Liubao brick tea (LBT)	1448:1469	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	5	73	theme	tea	1786:1788	arg1	proteins					1790:1797	tea proteins	1786:1797	tea proteins	1786:1797	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	1	74	theme	major	241:245	arg1	teas					247:250	the six major teas	233:250	the six major teas	233:250	BACKGROUND Dark tea, comprising one of the six major teas, has many biological activities, which originate from their active substrates, such as polyphenols, polysaccharides, and so on.
34018615	3	75	theme	hypoglycemic	692:703	arg1	activity					705:712	hypoglycemic activity	692:712	hypoglycemic activity in type 2 diabetes mellitus mice	692:745	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	1	76	contain	has	253:255	arg2	activities					273:282	many biological activities	257:282	many biological activities	257:282	BACKGROUND Dark tea, comprising one of the six major teas, has many biological activities, which originate from their active substrates, such as polyphenols, polysaccharides, and so on.
34018615	1	76	contain	has	253:255	arg1	tea					210:212	BACKGROUND Dark tea	194:212	BACKGROUND Dark tea	194:212	BACKGROUND Dark tea, comprising one of the six major teas, has many biological activities, which originate from their active substrates, such as polyphenols, polysaccharides, and so on.
34018615	5	77	dep	possessed	1492:1500	arg1	whereas					1614:1620	whereas	1614:1620	whereas	1614:1620	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	4	78	theme	resistance	1101:1110	arg1	activity					1065:1072	in vivo hypoglycemic activity	1044:1072	in vivo hypoglycemic activity	1044:1072	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	4	78	theme	resistance	1101:1110	arg1	activities					1032:1041	good glycosidase inhibition and free radical scavenging activities	976:1041	good glycosidase inhibition and free radical scavenging activities	976:1041	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	4	78	theme	resistance	1101:1110	arg1	alleviation					1078:1088	alleviation	1078:1088	alleviation of insulin resistance	1078:1110	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	3	79	theme	glycosidase	625:635	arg1	composition					612:622	the phytochemical composition	594:622	the phytochemical composition	594:622	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	3	79	theme	glycosidase	625:635	arg1	inhibition					637:646	glycosidase inhibition	625:646	glycosidase inhibition	625:646	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	4	80	theme	hypoglycemic	1052:1063	arg1	activity					1065:1072	in vivo hypoglycemic activity	1044:1072	in vivo hypoglycemic activity	1044:1072	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	6	81	theme	CONCLUSION	1877:1886	arg1	candidate					1920:1928	a highly attractive candidate	1900:1928	a highly attractive candidate for developing antidiabetic food, LBT	1900:1966	CONCLUSION Dark tea is a highly attractive candidate for developing antidiabetic food, LBT and PET may be good natural sources of agricultural products with anti-diabetic effects.
34018615	6	81	theme	CONCLUSION	1877:1886	arg1	tea					1893:1895	CONCLUSION Dark tea	1877:1895	CONCLUSION Dark tea	1877:1895	CONCLUSION Dark tea is a highly attractive candidate for developing antidiabetic food, LBT and PET may be good natural sources of agricultural products with anti-diabetic effects.
34018615	5	82	theme	Fuzhuan	1646:1652	arg1	tea					1660:1662	Fuzhuan brick tea	1646:1662	Fuzhuan brick tea	1646:1662	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	2	83	theme	potential	495:503	arg1	role					505:508	potential role	495:508	potential role	495:508	The hypoglycemic effect is one of its most prominent activities, although less is known about their evaluation and potential role in the hypoglycemic mechanism.
34018615	4	84	theme	inflammatory	1408:1419	arg1	levels					1428:1433	inflammatory factor levels	1408:1433	inflammatory factor levels	1408:1433	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	1	85	theme	Dark	205:208	arg1	tea					210:212	BACKGROUND Dark tea	194:212	BACKGROUND Dark tea	194:212	BACKGROUND Dark tea, comprising one of the six major teas, has many biological activities, which originate from their active substrates, such as polyphenols, polysaccharides, and so on.
34018615	5	86	theme	Qingzhuan	1622:1630	arg1	tea					1638:1640	Qingzhuan brick tea	1622:1640	Qingzhuan brick tea	1622:1640	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	5	87	theme	tea	1845:1847	arg1	pigments					1849:1856	tea pigments	1845:1856	tea pigments	1845:1856	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	2	88	theme	hypoglycemic	517:528	arg1	mechanism					530:538	the hypoglycemic mechanism	513:538	the hypoglycemic mechanism	513:538	The hypoglycemic effect is one of its most prominent activities, although less is known about their evaluation and potential role in the hypoglycemic mechanism.
34018615	4	89	theme	3-kinase-Akt-perixisome	1162:1184	arg1	pathway					1237:1243	the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway	1137:1243	the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels	1137:1433	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	6	90	theme	antidiabetic	1945:1956	arg1	LBT					1964:1966	antidiabetic food, LBT	1945:1966	LBT	1964:1966	CONCLUSION Dark tea is a highly attractive candidate for developing antidiabetic food, LBT and PET may be good natural sources of agricultural products with anti-diabetic effects.
34018615	3	91	theme	mellitus	733:740	arg1	mice					742:745	type 2 diabetes mellitus mice	717:745	type 2 diabetes mellitus mice	717:745	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	4	92	theme	oxidative	1387:1395	arg1	stress					1397:1402	oxidative stress	1387:1402	oxidative stress	1387:1402	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	0	93	theme	glucose	77:83	arg1	regulation					57:66	regulation	57:66	regulation	57:66	Comparative evaluation for phytochemical composition and regulation of blood glucose, hepatic oxidative stress and insulin resistance in mice and HepG2 models of four typical Chinese dark teas.
34018615	0	93	theme	glucose	77:83	arg1	composition					41:51	phytochemical composition	27:51	phytochemical composition	27:51	Comparative evaluation for phytochemical composition and regulation of blood glucose, hepatic oxidative stress and insulin resistance in mice and HepG2 models of four typical Chinese dark teas.
34018615	3	94	theme	present	556:562	arg1	study					564:568	the present study	552:568	the present study	552:568	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	3	95	theme	HepG2	800:804	arg1	cells					806:810	HepG2 cells	800:810	HepG2 cells of four dark tea aqueous extracts	800:844	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	4	96	theme	aqueous	913:919	arg1	extracts					921:928	dark tea aqueous extracts	904:928	dark tea aqueous extracts	904:928	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	5	97	dep	had	1664:1666	arg1	explained					1722:1730	explained	1722:1730	may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements	1715:1874	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	6	98	theme	attractive	1909:1918	arg1	candidate					1920:1928	a highly attractive candidate	1900:1928	a highly attractive candidate for developing antidiabetic food, LBT	1900:1966	CONCLUSION Dark tea is a highly attractive candidate for developing antidiabetic food, LBT and PET may be good natural sources of agricultural products with anti-diabetic effects.
34018615	6	98	theme	attractive	1909:1918	arg1	tea					1893:1895	CONCLUSION Dark tea	1877:1895	CONCLUSION Dark tea	1877:1895	CONCLUSION Dark tea is a highly attractive candidate for developing antidiabetic food, LBT and PET may be good natural sources of agricultural products with anti-diabetic effects.
34018615	0	99	theme	hepatic	86:92	arg1	stress					104:109	hepatic oxidative stress	86:109	hepatic oxidative stress	86:109	Comparative evaluation for phytochemical composition and regulation of blood glucose, hepatic oxidative stress and insulin resistance in mice and HepG2 models of four typical Chinese dark teas.
34018615	3	100	theme	aqueous	829:835	arg1	extracts					837:844	four dark tea aqueous extracts	815:844	four dark tea aqueous extracts	815:844	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	2	101	theme	activities	433:442	arg1	activities					433:442	its most prominent activities	414:442	its most prominent activities	414:442	The hypoglycemic effect is one of its most prominent activities, although less is known about their evaluation and potential role in the hypoglycemic mechanism.
34018615	2	101	theme	activities	433:442	arg1	one					407:409	one	407:409	one	407:409	The hypoglycemic effect is one of its most prominent activities, although less is known about their evaluation and potential role in the hypoglycemic mechanism.
34018615	2	101	theme	activities	433:442	arg1	effect					397:402	The hypoglycemic effect	380:402	The hypoglycemic effect	380:402	The hypoglycemic effect is one of its most prominent activities, although less is known about their evaluation and potential role in the hypoglycemic mechanism.
34018615	3	102	theme	resistance	786:795	arg1	activities					676:685	free radical scavenging activities	652:685	free radical scavenging activities	652:685	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	3	102	theme	resistance	786:795	arg1	activity					705:712	hypoglycemic activity	692:712	hypoglycemic activity in type 2 diabetes mellitus mice	692:745	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	3	102	theme	resistance	786:795	arg1	alleviation					763:773	the alleviation	759:773	the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts	759:844	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	3	102	theme	resistance	786:795	arg1	composition					612:622	the phytochemical composition	594:622	the phytochemical composition	594:622	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	3	102	theme	resistance	786:795	arg1	inhibition					637:646	glycosidase inhibition	625:646	glycosidase inhibition	625:646	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	0	103	theme	stress	104:109	arg1	regulation					57:66	regulation	57:66	regulation	57:66	Comparative evaluation for phytochemical composition and regulation of blood glucose, hepatic oxidative stress and insulin resistance in mice and HepG2 models of four typical Chinese dark teas.
34018615	0	103	theme	stress	104:109	arg1	composition					41:51	phytochemical composition	27:51	phytochemical composition	27:51	Comparative evaluation for phytochemical composition and regulation of blood glucose, hepatic oxidative stress and insulin resistance in mice and HepG2 models of four typical Chinese dark teas.
34018615	3	104	theme	dark	820:823	arg1	extracts					837:844	four dark tea aqueous extracts	815:844	four dark tea aqueous extracts	815:844	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	6	105	theme	good	1983:1986	arg1	sources					1996:2002	good natural sources	1983:2002	good natural sources of agricultural products with anti-diabetic effects	1983:2054	CONCLUSION Dark tea is a highly attractive candidate for developing antidiabetic food, LBT and PET may be good natural sources of agricultural products with anti-diabetic effects.
34018615	0	106	theme	insulin	115:121	arg1	resistance					123:132	insulin resistance	115:132	insulin resistance	115:132	Comparative evaluation for phytochemical composition and regulation of blood glucose, hepatic oxidative stress and insulin resistance in mice and HepG2 models of four typical Chinese dark teas.
34018615	4	107	theme	dark	904:907	arg1	extracts					921:928	dark tea aqueous extracts	904:928	dark tea aqueous extracts	904:928	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	4	108	theme	receptor	1210:1217	arg1	pathway					1237:1243	the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway	1137:1243	the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels	1137:1433	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	5	109	theme	scavenging	1688:1697	arg1	activity					1699:1706	better free radical scavenging activity	1668:1706	better free radical scavenging activity	1668:1706	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	5	110	theme	improved	1576:1583	arg1	resistance					1593:1602	improved insulin resistance	1576:1602	improved insulin resistance activity	1576:1611	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	4	111	theme	signaling	1227:1235	arg1	pathway					1237:1243	the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway	1137:1243	the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels	1137:1433	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	0	112	theme	phytochemical	27:39	arg1	composition					41:51	phytochemical composition	27:51	phytochemical composition	27:51	Comparative evaluation for phytochemical composition and regulation of blood glucose, hepatic oxidative stress and insulin resistance in mice and HepG2 models of four typical Chinese dark teas.
34018615	2	113	theme	hypoglycemic	384:395	arg1	activities					433:442	its most prominent activities	414:442	its most prominent activities	414:442	The hypoglycemic effect is one of its most prominent activities, although less is known about their evaluation and potential role in the hypoglycemic mechanism.
34018615	2	113	theme	hypoglycemic	384:395	arg1	one					407:409	one	407:409	one	407:409	The hypoglycemic effect is one of its most prominent activities, although less is known about their evaluation and potential role in the hypoglycemic mechanism.
34018615	2	113	theme	hypoglycemic	384:395	arg1	effect					397:402	The hypoglycemic effect	380:402	The hypoglycemic effect	380:402	The hypoglycemic effect is one of its most prominent activities, although less is known about their evaluation and potential role in the hypoglycemic mechanism.
34018615	1	114	theme	biological	262:271	arg1	activities					273:282	many biological activities	257:282	many biological activities	257:282	BACKGROUND Dark tea, comprising one of the six major teas, has many biological activities, which originate from their active substrates, such as polyphenols, polysaccharides, and so on.
34018615	3	115	from	resistance	786:795	arg1	cells					806:810	HepG2 cells	800:810	HepG2 cells of four dark tea aqueous extracts	800:844	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
34018615	4	116	theme	enzyme	1302:1307	arg1	activities					1309:1318	the key enzyme activities	1294:1318	the key enzyme activities related to glucose metabolism and antioxidant activity	1294:1373	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	5	117	theme	free	1675:1678	arg1	activity					1699:1706	better free radical scavenging activity	1668:1706	better free radical scavenging activity	1668:1706	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	4	118	theme	lipid	1269:1273	arg1	metabolism					1275:1284	lipid metabolism	1269:1284	lipid metabolism	1269:1284	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	4	119	theme	scavenging	1021:1030	arg1	activities					1032:1041	good glycosidase inhibition and free radical scavenging activities	976:1041	good glycosidase inhibition and free radical scavenging activities	976:1041	The results showed that the phytochemical composition of dark tea aqueous extracts was significantly different, and they all had good glycosidase inhibition and free radical scavenging activities, in vivo hypoglycemic activity and alleviation of insulin resistance, and could also activate the phosphatidylinositol 3-kinase-Akt-perixisome proliferation-activated receptor cascade signaling pathway to regulate glucose and lipid metabolism, change the key enzyme activities related to glucose metabolism and antioxidant activity, and reduce oxidative stress and inflammatory factor levels.
34018615	5	120	theme	glycosidase	1509:1519	arg1	activity					1532:1539	better glycosidase inhibitory activity	1502:1539	better glycosidase inhibitory activity	1502:1539	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	6	121	theme	agricultural	2007:2018	arg1	products					2020:2027	agricultural products	2007:2027	agricultural products with anti-diabetic effects	2007:2054	CONCLUSION Dark tea is a highly attractive candidate for developing antidiabetic food, LBT and PET may be good natural sources of agricultural products with anti-diabetic effects.
34018615	5	122	theme	phytochemical	1750:1762	arg1	catechins					1830:1838	catechins	1830:1838	catechins	1830:1838	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	5	122	theme	phytochemical	1750:1762	arg1	elements					1867:1874	some elements	1862:1874	some elements	1862:1874	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	5	122	theme	phytochemical	1750:1762	arg1	proteins					1790:1797	tea proteins	1786:1797	tea proteins	1786:1797	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	5	122	theme	phytochemical	1750:1762	arg1	polysaccharides					1800:1814	polysaccharides	1800:1814	polysaccharides	1800:1814	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	5	122	theme	phytochemical	1750:1762	arg1	compositions					1764:1775	their distinct phytochemical compositions	1735:1775	their distinct phytochemical compositions	1735:1775	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	5	122	theme	phytochemical	1750:1762	arg1	pigments					1849:1856	tea pigments	1845:1856	tea pigments	1845:1856	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	5	122	theme	phytochemical	1750:1762	arg1	polyphenols					1817:1827	polyphenols	1817:1827	polyphenols	1817:1827	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	2	123	from	role	505:508	arg1	mechanism					530:538	the hypoglycemic mechanism	513:538	the hypoglycemic mechanism	513:538	The hypoglycemic effect is one of its most prominent activities, although less is known about their evaluation and potential role in the hypoglycemic mechanism.
34018615	5	124	theme	insulin	1585:1591	arg1	resistance					1593:1602	improved insulin resistance	1576:1602	improved insulin resistance activity	1576:1611	Among them, Liubao brick tea (LBT) and Pu-erh tea (PET) possessed better glycosidase inhibitory activity, in vivo hypoglycemic activity and improved insulin resistance activity, whereas Qingzhuan brick tea and Fuzhuan brick tea had better free radical scavenging activity, which may be explained by their distinct phytochemical compositions, such as tea proteins, polysaccharides, polyphenols, catechins, and tea pigments and some elements.
34018615	3	125	theme	radical	657:663	arg1	scavenging					665:674	free radical scavenging	652:674	free radical scavenging activities	652:685	RESULTS In the present study, we separately analyzed the phytochemical composition, glycosidase inhibition and free radical scavenging activities, and hypoglycemic activity in type 2 diabetes mellitus mice, as well as the alleviation of insulin resistance in HepG2 cells of four dark tea aqueous extracts.
31935418	4	0	theme	Response	553:560	arg1	methodology					570:580	Response surface methodology	553:580	Response surface methodology	553:580	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	2	1	theme	cABC	342:345	arg1	enzyme					349:354	cABC I enzyme	342:354	cABC I enzyme	342:354	In this study, cABC I enzyme was immobilized on the dextran-coated Fe3O4 nanoparticles through physical adsorption to improve the thermal stability.
31935418	1	2	from	temperature	314:324	arg1	stability					287:295	low thermal stability	275:295	low thermal stability at physiological temperature	275:324	Chondroitinase ABC I (cABC I) has received notable attention in treatment of spinal cord injuries and its application as therapeutics has been limited due to low thermal stability at physiological temperature.
31935418	4	3	theme	Experimental	689:700	arg1	results					702:708	Experimental results	689:708	Experimental results	689:708	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	4	theme	surface	562:568	arg1	methodology					570:580	Response surface methodology	553:580	Response surface methodology	553:580	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	5	theme	enzyme/support	749:762	arg1	h					805:805	incubation time 5.7 h	785:805	incubation time 5.7 h	785:805	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	5	theme	enzyme/support	749:762	arg1	conditions					839:848	the optimal immobilization conditions	812:848	the optimal immobilization conditions	812:848	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	5	theme	enzyme/support	749:762	arg1	pH					722:723	pH 6.3	722:727	pH 6.3	722:727	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	5	theme	enzyme/support	749:762	arg1	°C					745:746	temperature 24 °C	730:746	temperature 24 °C	730:746	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	5	theme	enzyme/support	749:762	arg1	ratio					769:773	enzyme/support mass ratio 1.27	749:778	enzyme/support mass ratio 1.27	749:778	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	6	theme	central	586:592	arg1	design					604:609	central composite design	586:609	central composite design	586:609	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	0	7	theme	in-vitro	99:106	arg1	release					108:114	its in-vitro release	95:114	its in-vitro release	95:114	Examination of chondroitinase ABC I immobilization onto dextran-coated Fe3O4 nanoparticles and its in-vitro release.
31935418	4	8	theme	optimal	816:822	arg1	h					805:805	incubation time 5.7 h	785:805	incubation time 5.7 h	785:805	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	8	theme	optimal	816:822	arg1	conditions					839:848	the optimal immobilization conditions	812:848	the optimal immobilization conditions	812:848	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	8	theme	optimal	816:822	arg1	pH					722:723	pH 6.3	722:727	pH 6.3	722:727	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	8	theme	optimal	816:822	arg1	°C					745:746	temperature 24 °C	730:746	temperature 24 °C	730:746	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	8	theme	optimal	816:822	arg1	ratio					769:773	enzyme/support mass ratio 1.27	749:778	enzyme/support mass ratio 1.27	749:778	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	9	dep	I.	686:687	arg1	showed					710:715	showed	710:715	showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions	710:848	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	6	10	theme	70	1039:1040	arg1	%					1042:1042	%	1042:1042	%	1042:1042	In-vitro cABC I release was studied under pH 7.5 and temperature 37 °C and the results indicated that 70 % release occurred after 9 h and the release mechanism was first-order kinetic model.
31935418	1	11	theme	notable	160:166	arg1	attention					168:176	notable attention	160:176	notable attention	160:176	Chondroitinase ABC I (cABC I) has received notable attention in treatment of spinal cord injuries and its application as therapeutics has been limited due to low thermal stability at physiological temperature.
31935418	4	12	theme	mass	764:767	arg1	h					805:805	incubation time 5.7 h	785:805	incubation time 5.7 h	785:805	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	12	theme	mass	764:767	arg1	conditions					839:848	the optimal immobilization conditions	812:848	the optimal immobilization conditions	812:848	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	12	theme	mass	764:767	arg1	pH					722:723	pH 6.3	722:727	pH 6.3	722:727	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	12	theme	mass	764:767	arg1	°C					745:746	temperature 24 °C	730:746	temperature 24 °C	730:746	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	12	theme	mass	764:767	arg1	ratio					769:773	enzyme/support mass ratio 1.27	749:778	enzyme/support mass ratio 1.27	749:778	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	6	13	theme	%	1042:1042	arg1	release					1044:1050	70 % release	1039:1050	70 % release	1039:1050	In-vitro cABC I release was studied under pH 7.5 and temperature 37 °C and the results indicated that 70 % release occurred after 9 h and the release mechanism was first-order kinetic model.
31935418	6	14	theme	cABC	946:949	arg1	release					953:959	In-vitro cABC I release	937:959	In-vitro cABC I release	937:959	In-vitro cABC I release was studied under pH 7.5 and temperature 37 °C and the results indicated that 70 % release occurred after 9 h and the release mechanism was first-order kinetic model.
31935418	4	15	theme	temperature	730:740	arg1	h					805:805	incubation time 5.7 h	785:805	incubation time 5.7 h	785:805	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	15	theme	temperature	730:740	arg1	conditions					839:848	the optimal immobilization conditions	812:848	the optimal immobilization conditions	812:848	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	15	theme	temperature	730:740	arg1	°C					745:746	temperature 24 °C	730:746	temperature 24 °C	730:746	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	15	theme	temperature	730:740	arg1	pH					722:723	pH 6.3	722:727	pH 6.3	722:727	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	15	theme	temperature	730:740	arg1	ratio					769:773	enzyme/support mass ratio 1.27	749:778	enzyme/support mass ratio 1.27	749:778	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	16	theme	immobilized	669:679	arg1	cABC					681:684	immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions	669:848	immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions	669:848	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	17	theme	immobilization	824:837	arg1	h					805:805	incubation time 5.7 h	785:805	incubation time 5.7 h	785:805	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	17	theme	immobilization	824:837	arg1	conditions					839:848	the optimal immobilization conditions	812:848	the optimal immobilization conditions	812:848	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	17	theme	immobilization	824:837	arg1	pH					722:723	pH 6.3	722:727	pH 6.3	722:727	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	17	theme	immobilization	824:837	arg1	°C					745:746	temperature 24 °C	730:746	temperature 24 °C	730:746	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	17	theme	immobilization	824:837	arg1	ratio					769:773	enzyme/support mass ratio 1.27	749:778	enzyme/support mass ratio 1.27	749:778	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	6	18	theme	kinetic	1113:1119	arg1	model					1121:1125	first-order kinetic model	1101:1125	first-order kinetic model	1101:1125	In-vitro cABC I release was studied under pH 7.5 and temperature 37 °C and the results indicated that 70 % release occurred after 9 h and the release mechanism was first-order kinetic model.
31935418	6	18	theme	kinetic	1113:1119	arg1	mechanism					1087:1095	the release mechanism	1075:1095	the release mechanism	1075:1095	In-vitro cABC I release was studied under pH 7.5 and temperature 37 °C and the results indicated that 70 % release occurred after 9 h and the release mechanism was first-order kinetic model.
31935418	0	19	theme	ABC	30:32	arg1	Examination					0:10	Examination	0:10	Examination of chondroitinase ABC I	0:34	Examination of chondroitinase ABC I immobilization onto dextran-coated Fe3O4 nanoparticles and its in-vitro release.
31935418	6	20	theme	I	951:951	arg1	release					953:959	In-vitro cABC I release	937:959	In-vitro cABC I release	937:959	In-vitro cABC I release was studied under pH 7.5 and temperature 37 °C and the results indicated that 70 % release occurred after 9 h and the release mechanism was first-order kinetic model.
31935418	3	21	theme	FTIR	538:541	arg1	analyses					543:550	FTIR analyses	538:550	FTIR analyses	538:550	The nanoparticles were characterized using XRD, SEM, VSM, and FTIR analyses.
31935418	2	22	theme	physical	422:429	arg1	adsorption					431:440	physical adsorption	422:440	physical adsorption to improve the thermal stability	422:473	In this study, cABC I enzyme was immobilized on the dextran-coated Fe3O4 nanoparticles through physical adsorption to improve the thermal stability.
31935418	1	23	theme	low	275:277	arg1	stability					287:295	low thermal stability	275:295	low thermal stability at physiological temperature	275:324	Chondroitinase ABC I (cABC I) has received notable attention in treatment of spinal cord injuries and its application as therapeutics has been limited due to low thermal stability at physiological temperature.
31935418	1	24	theme	Chondroitinase	117:130	arg1	ABC					132:134	Chondroitinase ABC I	117:136	Chondroitinase ABC I (cABC I)	117:145	Chondroitinase ABC I (cABC I) has received notable attention in treatment of spinal cord injuries and its application as therapeutics has been limited due to low thermal stability at physiological temperature.
31935418	1	24	theme	Chondroitinase	117:130	arg1	I					144:144	cABC I	139:144	cABC I	139:144	Chondroitinase ABC I (cABC I) has received notable attention in treatment of spinal cord injuries and its application as therapeutics has been limited due to low thermal stability at physiological temperature.
31935418	1	25	theme	spinal	194:199	arg1	injuries					206:213	spinal cord injuries	194:213	spinal cord injuries	194:213	Chondroitinase ABC I (cABC I) has received notable attention in treatment of spinal cord injuries and its application as therapeutics has been limited due to low thermal stability at physiological temperature.
31935418	1	26	theme	thermal	279:285	arg1	stability					287:295	low thermal stability	275:295	low thermal stability at physiological temperature	275:324	Chondroitinase ABC I (cABC I) has received notable attention in treatment of spinal cord injuries and its application as therapeutics has been limited due to low thermal stability at physiological temperature.
31935418	1	27	theme	cord	201:204	arg1	injuries					206:213	spinal cord injuries	194:213	spinal cord injuries	194:213	Chondroitinase ABC I (cABC I) has received notable attention in treatment of spinal cord injuries and its application as therapeutics has been limited due to low thermal stability at physiological temperature.
31935418	2	28	theme	Fe3O4	394:398	arg1	nanoparticles					400:412	the dextran-coated Fe3O4 nanoparticles	375:412	the dextran-coated Fe3O4 nanoparticles	375:412	In this study, cABC I enzyme was immobilized on the dextran-coated Fe3O4 nanoparticles through physical adsorption to improve the thermal stability.
31935418	6	29	theme	In-vitro	937:944	arg1	release					953:959	In-vitro cABC I release	937:959	In-vitro cABC I release	937:959	In-vitro cABC I release was studied under pH 7.5 and temperature 37 °C and the results indicated that 70 % release occurred after 9 h and the release mechanism was first-order kinetic model.
31935418	4	30	theme	time	796:799	arg1	pH					722:723	pH 6.3	722:727	pH 6.3	722:727	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	30	theme	time	796:799	arg1	conditions					839:848	the optimal immobilization conditions	812:848	the optimal immobilization conditions	812:848	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	30	theme	time	796:799	arg1	h					805:805	incubation time 5.7 h	785:805	incubation time 5.7 h	785:805	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	30	theme	time	796:799	arg1	°C					745:746	temperature 24 °C	730:746	temperature 24 °C	730:746	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	30	theme	time	796:799	arg1	ratio					769:773	enzyme/support mass ratio 1.27	749:778	enzyme/support mass ratio 1.27	749:778	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	1	31	theme	injuries	206:213	arg1	treatment					181:189	treatment	181:189	treatment of spinal cord injuries	181:213	Chondroitinase ABC I (cABC I) has received notable attention in treatment of spinal cord injuries and its application as therapeutics has been limited due to low thermal stability at physiological temperature.
31935418	2	32	theme	thermal	457:463	arg1	stability					465:473	the thermal stability	453:473	the thermal stability	453:473	In this study, cABC I enzyme was immobilized on the dextran-coated Fe3O4 nanoparticles through physical adsorption to improve the thermal stability.
31935418	0	33	theme	Fe3O4	71:75	arg1	nanoparticles					77:89	dextran-coated Fe3O4 nanoparticles	56:89	dextran-coated Fe3O4 nanoparticles	56:89	Examination of chondroitinase ABC I immobilization onto dextran-coated Fe3O4 nanoparticles and its in-vitro release.
31935418	6	34	theme	release	1079:1085	arg1	model					1121:1125	first-order kinetic model	1101:1125	first-order kinetic model	1101:1125	In-vitro cABC I release was studied under pH 7.5 and temperature 37 °C and the results indicated that 70 % release occurred after 9 h and the release mechanism was first-order kinetic model.
31935418	6	34	theme	release	1079:1085	arg1	mechanism					1087:1095	the release mechanism	1075:1095	the release mechanism	1075:1095	In-vitro cABC I release was studied under pH 7.5 and temperature 37 °C and the results indicated that 70 % release occurred after 9 h and the release mechanism was first-order kinetic model.
31935418	5	35	theme	immobilized	890:900	arg1	cABC					902:905	immobilized cABC I	890:907	immobilized cABC I	890:907	It was found that thermal stability of immobilized cABC I was significantly improved.
31935418	1	36	theme	physiological	300:312	arg1	temperature					314:324	physiological temperature	300:324	physiological temperature	300:324	Chondroitinase ABC I (cABC I) has received notable attention in treatment of spinal cord injuries and its application as therapeutics has been limited due to low thermal stability at physiological temperature.
31935418	5	37	theme	cABC	902:905	arg1	stability					877:885	thermal stability	869:885	thermal stability of immobilized cABC I	869:907	It was found that thermal stability of immobilized cABC I was significantly improved.
31935418	6	38	theme	first-order	1101:1111	arg1	model					1121:1125	first-order kinetic model	1101:1125	first-order kinetic model	1101:1125	In-vitro cABC I release was studied under pH 7.5 and temperature 37 °C and the results indicated that 70 % release occurred after 9 h and the release mechanism was first-order kinetic model.
31935418	6	38	theme	first-order	1101:1111	arg1	mechanism					1087:1095	the release mechanism	1075:1095	the release mechanism	1075:1095	In-vitro cABC I release was studied under pH 7.5 and temperature 37 °C and the results indicated that 70 % release occurred after 9 h and the release mechanism was first-order kinetic model.
31935418	4	39	dep	cABC	681:684	arg1	I.					686:687	I.	686:687	I.	686:687	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	1	40	theme	cABC	139:142	arg1	ABC					132:134	Chondroitinase ABC I	117:136	Chondroitinase ABC I (cABC I)	117:145	Chondroitinase ABC I (cABC I) has received notable attention in treatment of spinal cord injuries and its application as therapeutics has been limited due to low thermal stability at physiological temperature.
31935418	1	40	theme	cABC	139:142	arg1	I					144:144	cABC I	139:144	cABC I	139:144	Chondroitinase ABC I (cABC I) has received notable attention in treatment of spinal cord injuries and its application as therapeutics has been limited due to low thermal stability at physiological temperature.
31935418	4	41	theme	composite	594:602	arg1	design					604:609	central composite design	586:609	central composite design	586:609	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	2	42	theme	dextran-coated	379:392	arg1	nanoparticles					400:412	the dextran-coated Fe3O4 nanoparticles	375:412	the dextran-coated Fe3O4 nanoparticles	375:412	In this study, cABC I enzyme was immobilized on the dextran-coated Fe3O4 nanoparticles through physical adsorption to improve the thermal stability.
31935418	4	43	theme	incubation	785:794	arg1	pH					722:723	pH 6.3	722:727	pH 6.3	722:727	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	43	theme	incubation	785:794	arg1	conditions					839:848	the optimal immobilization conditions	812:848	the optimal immobilization conditions	812:848	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	43	theme	incubation	785:794	arg1	h					805:805	incubation time 5.7 h	785:805	incubation time 5.7 h	785:805	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	43	theme	incubation	785:794	arg1	°C					745:746	temperature 24 °C	730:746	temperature 24 °C	730:746	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	4	43	theme	incubation	785:794	arg1	ratio					769:773	enzyme/support mass ratio 1.27	749:778	enzyme/support mass ratio 1.27	749:778	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
31935418	5	44	theme	thermal	869:875	arg1	stability					877:885	thermal stability	869:885	thermal stability of immobilized cABC I	869:907	It was found that thermal stability of immobilized cABC I was significantly improved.
31935418	6	45	theme	temperature	990:1000	arg1	°C					1005:1006	temperature 37 °C	990:1006	temperature 37 °C	990:1006	In-vitro cABC I release was studied under pH 7.5 and temperature 37 °C and the results indicated that 70 % release occurred after 9 h and the release mechanism was first-order kinetic model.
31935418	0	46	theme	dextran-coated	56:69	arg1	nanoparticles					77:89	dextran-coated Fe3O4 nanoparticles	56:89	dextran-coated Fe3O4 nanoparticles	56:89	Examination of chondroitinase ABC I immobilization onto dextran-coated Fe3O4 nanoparticles and its in-vitro release.
31935418	0	47	theme	chondroitinase	15:28	arg1	ABC					30:32	chondroitinase ABC I	15:34	chondroitinase ABC I	15:34	Examination of chondroitinase ABC I immobilization onto dextran-coated Fe3O4 nanoparticles and its in-vitro release.
31935418	4	48	theme	cABC	681:684	arg1	activity					657:664	the activity	653:664	the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions	653:848	Response surface methodology and central composite design were employed to assess factors affecting the activity of immobilized cABC I. Experimental results showed that pH 6.3, temperature 24 °C, enzyme/support mass ratio 1.27, and incubation time 5.7 h were the optimal immobilization conditions.
32611401	17	0	theme	bacterial	2244:2252	arg1	DNA					2254:2256	bacterial DNA	2244:2256	bacterial DNA	2244:2256	CONCLUSION Mycobacterial antigens were detectable in PFMC from tuberculous pleural effusions, even in cases where viable mycobacteria or bacterial DNA were not always detected.
32611401	3	1	theme	mycobacterial	511:523	arg1	antigens					525:532	various mycobacterial antigens	503:532	various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB	503:725	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	15	2	theme	CD4+	1810:1813	arg1	counts					1822:1827	low CD4+ T-cell counts	1806:1827	low CD4+ T-cell counts	1806:1827	Cases with low CD4+ T-cell counts had higher bacterial and antigen burden.
32611401	3	3	theme	pleural	716:722	arg1	TB					724:725	pleural TB	716:725	pleural TB	716:725	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	3	4	from	antigens	525:532	arg1	potential					658:666	the diagnostic potential	643:666	the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB	643:725	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	3	4	from	antigens	525:532	arg1	presence					617:624	the presence	613:624	the presence of antigens	613:636	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	3	4	from	antigens	525:532	arg1	effusions					545:553	pleural effusions	537:553	pleural effusions	537:553	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	3	4	from	antigens	525:532	arg1	impact					560:565	the impact	556:565	the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens	556:636	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	8	5	theme	composite	1249:1257	arg1	reference					1259:1267	a composite reference	1247:1267	a composite reference standard	1247:1276	Patients were categorized as pleural TB or non-TB cases using a composite reference standard.
32611401	16	6	theme	real-time	2092:2100	arg1	PCR					2102:2104	real-time PCR	2092:2104	real-time PCR	2092:2104	By combining detection of secreted antigen or LAM, the sensitivity and specificity to diagnose pleural TB was 56 and 78%, respectively, as compared to 41 and 100% for culture, 53 and 89% for nested PCR, and 6 and 100% for real-time PCR.
32611401	10	7	theme	pleural	1431:1437	arg1	TB					1439:1440	pleural TB	1431:1440	pleural TB	1431:1440	RESULTS A total of 41 patients were enrolled, of which 32 were classified as pleural TB and 9 as non-TB.
32611401	10	7	theme	pleural	1431:1437	arg1	32					1409:1410	32	1409:1410	32	1409:1410	RESULTS A total of 41 patients were enrolled, of which 32 were classified as pleural TB and 9 as non-TB.
32611401	8	8	theme	standard	1269:1276	arg1	reference					1259:1267	a composite reference	1247:1267	a composite reference standard	1247:1276	Patients were categorized as pleural TB or non-TB cases using a composite reference standard.
32611401	17	9	from	PFMC	2160:2163	arg1	detectable					2146:2155	detectable	2146:2155	detectable	2146:2155	CONCLUSION Mycobacterial antigens were detectable in PFMC from tuberculous pleural effusions, even in cases where viable mycobacteria or bacterial DNA were not always detected.
32611401	3	10	theme	infection	574:582	arg1	potential					658:666	the diagnostic potential	643:666	the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB	643:725	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	3	10	theme	infection	574:582	arg1	effusions					545:553	pleural effusions	537:553	pleural effusions	537:553	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	3	10	theme	infection	574:582	arg1	impact					560:565	the impact	556:565	the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens	556:636	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	16	11	theme	pleural	1965:1971	arg1	TB					1973:1974	pleural TB	1965:1974	pleural TB	1965:1974	By combining detection of secreted antigen or LAM, the sensitivity and specificity to diagnose pleural TB was 56 and 78%, respectively, as compared to 41 and 100% for culture, 53 and 89% for nested PCR, and 6 and 100% for real-time PCR.
32611401	8	12	theme	non-TB	1228:1233	arg1	Patients					1185:1192	Patients	1185:1192	Patients	1185:1192	Patients were categorized as pleural TB or non-TB cases using a composite reference standard.
32611401	8	12	theme	non-TB	1228:1233	arg1	cases					1235:1239	non-TB cases	1228:1239	non-TB cases	1228:1239	Patients were categorized as pleural TB or non-TB cases using a composite reference standard.
32611401	0	13	from	tuberculosis	78:89	arg1	patients					110:117	HIV co-infected patients	94:117	HIV co-infected patients	94:117	Mycobacterial antigens in pleural fluid mononuclear cells to diagnose pleural tuberculosis in HIV co-infected patients.
32611401	16	14	dep	sensitivity	1925:1935	arg1	diagnose					1956:1963	diagnose	1956:1963	to diagnose pleural TB	1953:1974	By combining detection of secreted antigen or LAM, the sensitivity and specificity to diagnose pleural TB was 56 and 78%, respectively, as compared to 41 and 100% for culture, 53 and 89% for nested PCR, and 6 and 100% for real-time PCR.
32611401	16	14	dep	sensitivity	1925:1935	arg1	the					1921:1923	the	1921:1923	the	1921:1923	By combining detection of secreted antigen or LAM, the sensitivity and specificity to diagnose pleural TB was 56 and 78%, respectively, as compared to 41 and 100% for culture, 53 and 89% for nested PCR, and 6 and 100% for real-time PCR.
32611401	11	15	theme	confirmed	1489:1497	arg1	TB					1507:1508	culture confirmed pleural TB	1481:1508	culture confirmed pleural TB	1481:1508	Thirteen patients had culture confirmed pleural TB, 26 (81%) were HIV-TB co-infected, and 64% had < 100 CD4+ T-cells/microL.
32611401	3	16	theme	depletion	600:608	arg1	potential					658:666	the diagnostic potential	643:666	the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB	643:725	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	3	16	theme	depletion	600:608	arg1	effusions					545:553	pleural effusions	537:553	pleural effusions	537:553	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	3	16	theme	depletion	600:608	arg1	impact					560:565	the impact	556:565	the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens	556:636	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	1	17	theme	cases	224:228	arg1	one-half					209:216	approximately one-half	195:216	approximately one-half of TB cases	195:228	BACKGROUND Extra pulmonary manifestation of tuberculosis (TB) accounts for approximately one-half of TB cases in HIV-infected individuals with pleural TB as the second most common location.
32611401	4	18	theme	adenosine	889:897	arg1	deaminase					899:907	adenosine deaminase	889:907	adenosine deaminase	889:907	METHODS Pleural fluid specimens were collected from patients presenting with clinically suspected pleural TB, and processed routinely for culture, cytology, and adenosine deaminase activity analysis.
32611401	18	19	theme	LAM	2328:2330	arg1	detection					2332:2340	secreted antigen and LAM detection	2307:2340	secreted antigen and LAM detection by immunocytochemistry	2307:2363	Thus, a combination of secreted antigen and LAM detection by immunocytochemistry may be a complement to acid-fast staining and contribute to rapid and accurate diagnosis of pleural TB.
32611401	3	20	theme	CD4+	588:591	arg1	depletion					600:608	CD4+ T-cell depletion	588:608	CD4+ T-cell depletion	588:608	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	1	21	theme	Extra	131:135	arg1	manifestation					147:159	BACKGROUND Extra pulmonary manifestation	120:159	BACKGROUND Extra pulmonary manifestation of tuberculosis (TB)	120:180	BACKGROUND Extra pulmonary manifestation of tuberculosis (TB) accounts for approximately one-half of TB cases in HIV-infected individuals with pleural TB as the second most common location.
32611401	11	22	contain	had	1553:1555	arg2	T-cells/microL					1568:1581	< 100 CD4+ T-cells/microL	1557:1581	< 100 CD4+ T-cells/microL	1557:1581	Thirteen patients had culture confirmed pleural TB, 26 (81%) were HIV-TB co-infected, and 64% had < 100 CD4+ T-cells/microL.
32611401	11	22	contain	had	1553:1555	arg1	%					1551:1551	64%	1549:1551	64%	1549:1551	Thirteen patients had culture confirmed pleural TB, 26 (81%) were HIV-TB co-infected, and 64% had < 100 CD4+ T-cells/microL.
32611401	4	23	theme	activity	909:916	arg1	analysis					918:925	activity analysis	909:925	activity analysis	909:925	METHODS Pleural fluid specimens were collected from patients presenting with clinically suspected pleural TB, and processed routinely for culture, cytology, and adenosine deaminase activity analysis.
32611401	11	24	theme	pleural	1499:1505	arg1	TB					1507:1508	culture confirmed pleural TB	1481:1508	culture confirmed pleural TB	1481:1508	Thirteen patients had culture confirmed pleural TB, 26 (81%) were HIV-TB co-infected, and 64% had < 100 CD4+ T-cells/microL.
32611401	1	25	theme	pulmonary	137:145	arg1	manifestation					147:159	BACKGROUND Extra pulmonary manifestation	120:159	BACKGROUND Extra pulmonary manifestation of tuberculosis (TB)	120:180	BACKGROUND Extra pulmonary manifestation of tuberculosis (TB) accounts for approximately one-half of TB cases in HIV-infected individuals with pleural TB as the second most common location.
32611401	17	26	theme	tuberculous	2170:2180	arg1	effusions					2190:2198	tuberculous pleural effusions	2170:2198	tuberculous pleural effusions	2170:2198	CONCLUSION Mycobacterial antigens were detectable in PFMC from tuberculous pleural effusions, even in cases where viable mycobacteria or bacterial DNA were not always detected.
32611401	11	27	theme	culture	1481:1487	arg1	TB					1507:1508	culture confirmed pleural TB	1481:1508	culture confirmed pleural TB	1481:1508	Thirteen patients had culture confirmed pleural TB, 26 (81%) were HIV-TB co-infected, and 64% had < 100 CD4+ T-cells/microL.
32611401	9	28	theme	mycobacterial	1298:1310	arg1	antigens					1312:1319	the mycobacterial antigens	1294:1319	the mycobacterial antigens	1294:1319	Performance of the mycobacterial antigens as diagnostic test was assessed.
32611401	1	29	theme	tuberculosis	164:175	arg1	manifestation					147:159	BACKGROUND Extra pulmonary manifestation	120:159	BACKGROUND Extra pulmonary manifestation of tuberculosis (TB)	120:180	BACKGROUND Extra pulmonary manifestation of tuberculosis (TB) accounts for approximately one-half of TB cases in HIV-infected individuals with pleural TB as the second most common location.
32611401	17	30	from	effusions	2190:2198	arg1	PFMC					2160:2163	PFMC	2160:2163	PFMC from tuberculous pleural effusions	2160:2198	CONCLUSION Mycobacterial antigens were detectable in PFMC from tuberculous pleural effusions, even in cases where viable mycobacteria or bacterial DNA were not always detected.
32611401	17	31	theme	CONCLUSION	2107:2116	arg1	antigens					2132:2139	CONCLUSION Mycobacterial antigens	2107:2139	CONCLUSION Mycobacterial antigens	2107:2139	CONCLUSION Mycobacterial antigens were detectable in PFMC from tuberculous pleural effusions, even in cases where viable mycobacteria or bacterial DNA were not always detected.
32611401	3	32	from	effusions	545:553	arg1	presence					617:624	the presence	613:624	the presence of antigens	613:636	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	0	33	theme	HIV	94:96	arg1	patients					110:117	HIV co-infected patients	94:117	HIV co-infected patients	94:117	Mycobacterial antigens in pleural fluid mononuclear cells to diagnose pleural tuberculosis in HIV co-infected patients.
32611401	6	34	theme	fluid	985:989	arg1	cells					1003:1007	Pleural fluid mononuclear cells	977:1007	Pleural fluid mononuclear cells (PFMC)	977:1014	Pleural fluid mononuclear cells (PFMC) were isolated, and cell smears were stained with acid-fast staining and immunocytochemistry for various mycobacterial antigens.
32611401	6	34	theme	fluid	985:989	arg1	PFMC					1010:1013	PFMC	1010:1013	PFMC	1010:1013	Pleural fluid mononuclear cells (PFMC) were isolated, and cell smears were stained with acid-fast staining and immunocytochemistry for various mycobacterial antigens.
32611401	4	35	theme	METHODS	728:734	arg1	specimens					750:758	METHODS Pleural fluid specimens	728:758	METHODS Pleural fluid specimens	728:758	METHODS Pleural fluid specimens were collected from patients presenting with clinically suspected pleural TB, and processed routinely for culture, cytology, and adenosine deaminase activity analysis.
32611401	0	36	theme	Mycobacterial	0:12	arg1	antigens					14:21	Mycobacterial antigens	0:21	Mycobacterial antigens in pleural fluid mononuclear cells	0:56	Mycobacterial antigens in pleural fluid mononuclear cells to diagnose pleural tuberculosis in HIV co-infected patients.
32611401	1	37	theme	second	281:286	arg1	location					300:307	the second most common location	277:307	the second most common location	277:307	BACKGROUND Extra pulmonary manifestation of tuberculosis (TB) accounts for approximately one-half of TB cases in HIV-infected individuals with pleural TB as the second most common location.
32611401	5	38	theme	CD4+	943:946	arg1	counts					955:960	CD4+ T-cell counts	943:960	CD4+ T-cell counts	943:960	HIV status and CD4+ T-cell counts were recorded.
32611401	16	39	theme	78	1987:1988	arg1	%					1989:1989	56 and 78%	1980:1989	%	1989:1989	By combining detection of secreted antigen or LAM, the sensitivity and specificity to diagnose pleural TB was 56 and 78%, respectively, as compared to 41 and 100% for culture, 53 and 89% for nested PCR, and 6 and 100% for real-time PCR.
32611401	4	40	theme	fluid	744:748	arg1	specimens					750:758	METHODS Pleural fluid specimens	728:758	METHODS Pleural fluid specimens	728:758	METHODS Pleural fluid specimens were collected from patients presenting with clinically suspected pleural TB, and processed routinely for culture, cytology, and adenosine deaminase activity analysis.
32611401	16	41	theme	secreted	1896:1903	arg1	antigen					1905:1911	antigen	1905:1911	antigen	1905:1911	By combining detection of secreted antigen or LAM, the sensitivity and specificity to diagnose pleural TB was 56 and 78%, respectively, as compared to 41 and 100% for culture, 53 and 89% for nested PCR, and 6 and 100% for real-time PCR.
32611401	1	42	theme	common	293:298	arg1	location					300:307	the second most common location	277:307	the second most common location	277:307	BACKGROUND Extra pulmonary manifestation of tuberculosis (TB) accounts for approximately one-half of TB cases in HIV-infected individuals with pleural TB as the second most common location.
32611401	12	43	theme	mycobacterial	1612:1624	arg1	antigens					1626:1633	Both secreted and cell-wall mycobacterial antigens	1584:1633	Both secreted and cell-wall mycobacterial antigens	1584:1633	Both secreted and cell-wall mycobacterial antigens were detected in PFMC.
32611401	6	44	theme	cell	1035:1038	arg1	smears					1040:1045	cell smears	1035:1045	cell smears	1035:1045	Pleural fluid mononuclear cells (PFMC) were isolated, and cell smears were stained with acid-fast staining and immunocytochemistry for various mycobacterial antigens.
32611401	18	45	theme	antigen	2316:2322	arg1	detection					2332:2340	secreted antigen and LAM detection	2307:2340	secreted antigen and LAM detection by immunocytochemistry	2307:2363	Thus, a combination of secreted antigen and LAM detection by immunocytochemistry may be a complement to acid-fast staining and contribute to rapid and accurate diagnosis of pleural TB.
32611401	4	46	theme	pleural	826:832	arg1	TB					834:835	clinically suspected pleural TB	805:835	clinically suspected pleural TB	805:835	METHODS Pleural fluid specimens were collected from patients presenting with clinically suspected pleural TB, and processed routinely for culture, cytology, and adenosine deaminase activity analysis.
32611401	0	47	theme	fluid	34:38	arg1	cells					52:56	pleural fluid mononuclear cells	26:56	pleural fluid mononuclear cells	26:56	Mycobacterial antigens in pleural fluid mononuclear cells to diagnose pleural tuberculosis in HIV co-infected patients.
32611401	14	48	theme	positive	1764:1771	arg1	culture					1773:1779	positive culture	1764:1779	positive culture	1764:1779	There was no direct correlation between positive culture and antigens.
32611401	2	49	theme	mycobacterial	348:360	arg1	antigens					362:369	mycobacterial antigens	348:369	mycobacterial antigens	348:369	Even though mycobacteria are cleared, mycobacterial antigens may persist in infected tissues, causing sustained inflammation and chronicity of the disease.
32611401	4	50	dep	culture	866:872	arg1	analysis					918:925	activity analysis	909:925	activity analysis	909:925	METHODS Pleural fluid specimens were collected from patients presenting with clinically suspected pleural TB, and processed routinely for culture, cytology, and adenosine deaminase activity analysis.
32611401	16	51	theme	antigen	1905:1911	arg1	detection					1883:1891	detection	1883:1891	detection of secreted antigen or LAM	1883:1918	By combining detection of secreted antigen or LAM, the sensitivity and specificity to diagnose pleural TB was 56 and 78%, respectively, as compared to 41 and 100% for culture, 53 and 89% for nested PCR, and 6 and 100% for real-time PCR.
32611401	3	52	theme	antigens	671:678	arg1	potential					658:666	the diagnostic potential	643:666	the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB	643:725	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	3	52	theme	antigens	671:678	arg1	effusions					545:553	pleural effusions	537:553	pleural effusions	537:553	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	3	52	theme	antigens	671:678	arg1	impact					560:565	the impact	556:565	the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens	556:636	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	3	53	theme	study	482:486	arg1	aim					470:472	The aim	466:472	The aim of this study	466:486	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	16	54	theme	LAM	1916:1918	arg1	detection					1883:1891	detection	1883:1891	detection of secreted antigen or LAM	1883:1918	By combining detection of secreted antigen or LAM, the sensitivity and specificity to diagnose pleural TB was 56 and 78%, respectively, as compared to 41 and 100% for culture, 53 and 89% for nested PCR, and 6 and 100% for real-time PCR.
32611401	1	55	with	individuals	246:256	arg1	TB					271:272	pleural TB	263:272	pleural TB as the second most common location	263:307	BACKGROUND Extra pulmonary manifestation of tuberculosis (TB) accounts for approximately one-half of TB cases in HIV-infected individuals with pleural TB as the second most common location.
32611401	6	56	theme	various	1112:1118	arg1	antigens					1134:1141	various mycobacterial antigens	1112:1141	various mycobacterial antigens	1112:1141	Pleural fluid mononuclear cells (PFMC) were isolated, and cell smears were stained with acid-fast staining and immunocytochemistry for various mycobacterial antigens.
32611401	17	57	theme	viable	2221:2226	arg1	mycobacteria					2228:2239	viable mycobacteria	2221:2239	viable mycobacteria	2221:2239	CONCLUSION Mycobacterial antigens were detectable in PFMC from tuberculous pleural effusions, even in cases where viable mycobacteria or bacterial DNA were not always detected.
32611401	18	58	theme	secreted	2307:2314	arg1	detection					2332:2340	secreted antigen and LAM detection	2307:2340	secreted antigen and LAM detection by immunocytochemistry	2307:2363	Thus, a combination of secreted antigen and LAM detection by immunocytochemistry may be a complement to acid-fast staining and contribute to rapid and accurate diagnosis of pleural TB.
32611401	15	59	theme	T-cell	1815:1820	arg1	counts					1822:1827	low CD4+ T-cell counts	1806:1827	low CD4+ T-cell counts	1806:1827	Cases with low CD4+ T-cell counts had higher bacterial and antigen burden.
32611401	3	60	theme	rapid	697:701	arg1	diagnosis					703:711	improved and rapid diagnosis	684:711	diagnosis	703:711	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	3	61	theme	various	503:509	arg1	antigens					525:532	various mycobacterial antigens	503:532	various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB	503:725	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	1	62	theme	pleural	263:269	arg1	TB					271:272	pleural TB	263:272	pleural TB as the second most common location	263:307	BACKGROUND Extra pulmonary manifestation of tuberculosis (TB) accounts for approximately one-half of TB cases in HIV-infected individuals with pleural TB as the second most common location.
32611401	16	63	theme	56	1980:1981	arg1	%					1989:1989	56 and 78%	1980:1989	%	1989:1989	By combining detection of secreted antigen or LAM, the sensitivity and specificity to diagnose pleural TB was 56 and 78%, respectively, as compared to 41 and 100% for culture, 53 and 89% for nested PCR, and 6 and 100% for real-time PCR.
32611401	15	64	theme	low	1806:1808	arg1	counts					1822:1827	low CD4+ T-cell counts	1806:1827	low CD4+ T-cell counts	1806:1827	Cases with low CD4+ T-cell counts had higher bacterial and antigen burden.
32611401	3	65	theme	improved	684:691	arg1	diagnosis					703:711	improved and rapid diagnosis	684:711	diagnosis	703:711	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	3	66	theme	TB	724:725	arg1	diagnosis					703:711	improved and rapid diagnosis	684:711	diagnosis	703:711	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	15	67	dep	bacterial	1840:1848	arg1	higher					1833:1838	higher	1833:1838	higher	1833:1838	Cases with low CD4+ T-cell counts had higher bacterial and antigen burden.
32611401	3	68	theme	pleural	537:543	arg1	effusions					545:553	pleural effusions	537:553	pleural effusions	537:553	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	18	69	theme	accurate	2435:2442	arg1	diagnosis					2444:2452	rapid and accurate diagnosis	2425:2452	rapid and accurate diagnosis of pleural TB	2425:2466	Thus, a combination of secreted antigen and LAM detection by immunocytochemistry may be a complement to acid-fast staining and contribute to rapid and accurate diagnosis of pleural TB.
32611401	3	70	theme	HIV	570:572	arg1	infection					574:582	HIV infection	570:582	HIV infection	570:582	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	12	71	located	detected	1640:1647	arg1	PFMC					1652:1655	PFMC	1652:1655	PFMC	1652:1655	Both secreted and cell-wall mycobacterial antigens were detected in PFMC.
32611401	12	71	located	detected	1640:1647	arg2	antigens					1626:1633	Both secreted and cell-wall mycobacterial antigens	1584:1633	Both secreted and cell-wall mycobacterial antigens	1584:1633	Both secreted and cell-wall mycobacterial antigens were detected in PFMC.
32611401	6	72	theme	Pleural	977:983	arg1	cells					1003:1007	Pleural fluid mononuclear cells	977:1007	Pleural fluid mononuclear cells (PFMC)	977:1014	Pleural fluid mononuclear cells (PFMC) were isolated, and cell smears were stained with acid-fast staining and immunocytochemistry for various mycobacterial antigens.
32611401	6	72	theme	Pleural	977:983	arg1	PFMC					1010:1013	PFMC	1010:1013	PFMC	1010:1013	Pleural fluid mononuclear cells (PFMC) were isolated, and cell smears were stained with acid-fast staining and immunocytochemistry for various mycobacterial antigens.
32611401	18	73	theme	TB	2465:2466	arg1	diagnosis					2444:2452	rapid and accurate diagnosis	2425:2452	rapid and accurate diagnosis of pleural TB	2425:2466	Thus, a combination of secreted antigen and LAM detection by immunocytochemistry may be a complement to acid-fast staining and contribute to rapid and accurate diagnosis of pleural TB.
32611401	18	74	theme	rapid	2425:2429	arg1	diagnosis					2444:2452	rapid and accurate diagnosis	2425:2452	rapid and accurate diagnosis of pleural TB	2425:2466	Thus, a combination of secreted antigen and LAM detection by immunocytochemistry may be a complement to acid-fast staining and contribute to rapid and accurate diagnosis of pleural TB.
32611401	10	75	dep	RESULTS	1354:1360	arg1	enrolled					1390:1397	enrolled	1390:1397	enrolled	1390:1397	RESULTS A total of 41 patients were enrolled, of which 32 were classified as pleural TB and 9 as non-TB.
32611401	8	76	theme	pleural	1214:1220	arg1	TB					1222:1223	pleural TB	1214:1223	pleural TB	1214:1223	Patients were categorized as pleural TB or non-TB cases using a composite reference standard.
32611401	8	76	theme	pleural	1214:1220	arg1	Patients					1185:1192	Patients	1185:1192	Patients	1185:1192	Patients were categorized as pleural TB or non-TB cases using a composite reference standard.
32611401	15	77	theme	antigen	1854:1860	arg1	burden					1862:1867	higher bacterial and antigen burden	1833:1867	burden	1862:1867	Cases with low CD4+ T-cell counts had higher bacterial and antigen burden.
32611401	3	78	theme	T-cell	593:598	arg1	depletion					600:608	CD4+ T-cell depletion	588:608	CD4+ T-cell depletion	588:608	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	1	79	theme	TB	221:222	arg1	cases					224:228	TB cases	221:228	TB cases	221:228	BACKGROUND Extra pulmonary manifestation of tuberculosis (TB) accounts for approximately one-half of TB cases in HIV-infected individuals with pleural TB as the second most common location.
32611401	17	80	theme	pleural	2182:2188	arg1	effusions					2190:2198	tuberculous pleural effusions	2170:2198	tuberculous pleural effusions	2170:2198	CONCLUSION Mycobacterial antigens were detectable in PFMC from tuberculous pleural effusions, even in cases where viable mycobacteria or bacterial DNA were not always detected.
32611401	1	81	theme	BACKGROUND	120:129	arg1	manifestation					147:159	BACKGROUND Extra pulmonary manifestation	120:159	BACKGROUND Extra pulmonary manifestation of tuberculosis (TB)	120:180	BACKGROUND Extra pulmonary manifestation of tuberculosis (TB) accounts for approximately one-half of TB cases in HIV-infected individuals with pleural TB as the second most common location.
32611401	3	82	from	impact	560:565	arg1	presence					617:624	the presence	613:624	the presence of antigens	613:636	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	5	83	theme	HIV	928:930	arg1	status					932:937	HIV status	928:937	HIV status	928:937	HIV status and CD4+ T-cell counts were recorded.
32611401	0	84	theme	pleural	70:76	arg1	tuberculosis					78:89	pleural tuberculosis	70:89	pleural tuberculosis in HIV co-infected patients	70:117	Mycobacterial antigens in pleural fluid mononuclear cells to diagnose pleural tuberculosis in HIV co-infected patients.
32611401	17	85	from	detectable	2146:2155	arg1	cases					2209:2213	cases	2209:2213	cases where viable mycobacteria or bacterial DNA were not always detected	2209:2281	CONCLUSION Mycobacterial antigens were detectable in PFMC from tuberculous pleural effusions, even in cases where viable mycobacteria or bacterial DNA were not always detected.
32611401	17	85	from	detectable	2146:2155	arg1	PFMC					2160:2163	PFMC	2160:2163	PFMC from tuberculous pleural effusions	2160:2198	CONCLUSION Mycobacterial antigens were detectable in PFMC from tuberculous pleural effusions, even in cases where viable mycobacteria or bacterial DNA were not always detected.
32611401	9	86	theme	antigens	1312:1319	arg1	Performance					1279:1289	Performance	1279:1289	Performance of the mycobacterial antigens as diagnostic test	1279:1338	Performance of the mycobacterial antigens as diagnostic test was assessed.
32611401	15	87	contain	had	1829:1831	arg2	bacterial					1840:1848	bacterial	1840:1848	bacterial	1840:1848	Cases with low CD4+ T-cell counts had higher bacterial and antigen burden.
32611401	15	87	contain	had	1829:1831	arg1	Cases					1795:1799	Cases	1795:1799	Cases with low CD4+ T-cell counts	1795:1827	Cases with low CD4+ T-cell counts had higher bacterial and antigen burden.
32611401	15	87	contain	had	1829:1831	arg2	burden					1862:1867	higher bacterial and antigen burden	1833:1867	burden	1862:1867	Cases with low CD4+ T-cell counts had higher bacterial and antigen burden.
32611401	0	88	from	antigens	14:21	arg1	cells					52:56	pleural fluid mononuclear cells	26:56	pleural fluid mononuclear cells	26:56	Mycobacterial antigens in pleural fluid mononuclear cells to diagnose pleural tuberculosis in HIV co-infected patients.
32611401	0	89	theme	co-infected	98:108	arg1	patients					110:117	HIV co-infected patients	94:117	HIV co-infected patients	94:117	Mycobacterial antigens in pleural fluid mononuclear cells to diagnose pleural tuberculosis in HIV co-infected patients.
32611401	9	90	theme	diagnostic	1324:1333	arg1	test					1335:1338	diagnostic test	1324:1338	diagnostic test	1324:1338	Performance of the mycobacterial antigens as diagnostic test was assessed.
32611401	6	91	theme	mononuclear	991:1001	arg1	cells					1003:1007	Pleural fluid mononuclear cells	977:1007	Pleural fluid mononuclear cells (PFMC)	977:1014	Pleural fluid mononuclear cells (PFMC) were isolated, and cell smears were stained with acid-fast staining and immunocytochemistry for various mycobacterial antigens.
32611401	6	91	theme	mononuclear	991:1001	arg1	PFMC					1010:1013	PFMC	1010:1013	PFMC	1010:1013	Pleural fluid mononuclear cells (PFMC) were isolated, and cell smears were stained with acid-fast staining and immunocytochemistry for various mycobacterial antigens.
32611401	6	92	theme	acid-fast	1065:1073	arg1	staining					1075:1082	acid-fast staining	1065:1082	acid-fast staining	1065:1082	Pleural fluid mononuclear cells (PFMC) were isolated, and cell smears were stained with acid-fast staining and immunocytochemistry for various mycobacterial antigens.
32611401	16	93	theme	nested	2061:2066	arg1	PCR					2068:2070	nested PCR	2061:2070	nested PCR	2061:2070	By combining detection of secreted antigen or LAM, the sensitivity and specificity to diagnose pleural TB was 56 and 78%, respectively, as compared to 41 and 100% for culture, 53 and 89% for nested PCR, and 6 and 100% for real-time PCR.
32611401	3	94	from	presence	617:624	arg1	antigens					525:532	various mycobacterial antigens	503:532	various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB	503:725	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	2	95	theme	disease	457:463	arg1	inflammation					422:433	sustained inflammation	412:433	sustained inflammation	412:433	Even though mycobacteria are cleared, mycobacterial antigens may persist in infected tissues, causing sustained inflammation and chronicity of the disease.
32611401	2	95	theme	disease	457:463	arg1	chronicity					439:448	chronicity	439:448	chronicity of the disease	439:463	Even though mycobacteria are cleared, mycobacterial antigens may persist in infected tissues, causing sustained inflammation and chronicity of the disease.
32611401	18	96	theme	acid-fast	2388:2396	arg1	staining					2398:2405	acid-fast staining	2388:2405	acid-fast staining	2388:2405	Thus, a combination of secreted antigen and LAM detection by immunocytochemistry may be a complement to acid-fast staining and contribute to rapid and accurate diagnosis of pleural TB.
32611401	2	97	theme	infected	386:393	arg1	tissues					395:401	infected tissues	386:401	infected tissues	386:401	Even though mycobacteria are cleared, mycobacterial antigens may persist in infected tissues, causing sustained inflammation and chronicity of the disease.
32611401	0	98	theme	pleural	26:32	arg1	cells					52:56	pleural fluid mononuclear cells	26:56	pleural fluid mononuclear cells	26:56	Mycobacterial antigens in pleural fluid mononuclear cells to diagnose pleural tuberculosis in HIV co-infected patients.
32611401	5	99	theme	T-cell	948:953	arg1	counts					955:960	CD4+ T-cell counts	943:960	CD4+ T-cell counts	943:960	HIV status and CD4+ T-cell counts were recorded.
32611401	12	100	theme	secreted	1589:1596	arg1	antigens					1626:1633	Both secreted and cell-wall mycobacterial antigens	1584:1633	Both secreted and cell-wall mycobacterial antigens	1584:1633	Both secreted and cell-wall mycobacterial antigens were detected in PFMC.
32611401	4	101	theme	Pleural	736:742	arg1	specimens					750:758	METHODS Pleural fluid specimens	728:758	METHODS Pleural fluid specimens	728:758	METHODS Pleural fluid specimens were collected from patients presenting with clinically suspected pleural TB, and processed routinely for culture, cytology, and adenosine deaminase activity analysis.
32611401	0	102	theme	mononuclear	40:50	arg1	cells					52:56	pleural fluid mononuclear cells	26:56	pleural fluid mononuclear cells	26:56	Mycobacterial antigens in pleural fluid mononuclear cells to diagnose pleural tuberculosis in HIV co-infected patients.
32611401	15	103	with	Cases	1795:1799	arg1	counts					1822:1827	low CD4+ T-cell counts	1806:1827	low CD4+ T-cell counts	1806:1827	Cases with low CD4+ T-cell counts had higher bacterial and antigen burden.
32611401	12	104	theme	cell-wall	1602:1610	arg1	antigens					1626:1633	Both secreted and cell-wall mycobacterial antigens	1584:1633	Both secreted and cell-wall mycobacterial antigens	1584:1633	Both secreted and cell-wall mycobacterial antigens were detected in PFMC.
32611401	18	105	theme	pleural	2457:2463	arg1	TB					2465:2466	pleural TB	2457:2466	pleural TB	2457:2466	Thus, a combination of secreted antigen and LAM detection by immunocytochemistry may be a complement to acid-fast staining and contribute to rapid and accurate diagnosis of pleural TB.
32611401	3	106	theme	antigens	629:636	arg1	presence					617:624	the presence	613:624	the presence of antigens	613:636	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	4	107	theme	suspected	816:824	arg1	TB					834:835	clinically suspected pleural TB	805:835	clinically suspected pleural TB	805:835	METHODS Pleural fluid specimens were collected from patients presenting with clinically suspected pleural TB, and processed routinely for culture, cytology, and adenosine deaminase activity analysis.
32611401	2	108	theme	sustained	412:420	arg1	inflammation					422:433	sustained inflammation	412:433	sustained inflammation	412:433	Even though mycobacteria are cleared, mycobacterial antigens may persist in infected tissues, causing sustained inflammation and chronicity of the disease.
32611401	17	109	from	cases	2209:2213	arg1	detectable					2146:2155	detectable	2146:2155	detectable	2146:2155	CONCLUSION Mycobacterial antigens were detectable in PFMC from tuberculous pleural effusions, even in cases where viable mycobacteria or bacterial DNA were not always detected.
32611401	17	110	theme	Mycobacterial	2118:2130	arg1	antigens					2132:2139	CONCLUSION Mycobacterial antigens	2107:2139	CONCLUSION Mycobacterial antigens	2107:2139	CONCLUSION Mycobacterial antigens were detectable in PFMC from tuberculous pleural effusions, even in cases where viable mycobacteria or bacterial DNA were not always detected.
32611401	14	111	theme	direct	1737:1742	arg1	correlation					1744:1754	no direct correlation	1734:1754	no direct correlation between positive culture and antigens	1734:1792	There was no direct correlation between positive culture and antigens.
32611401	11	112	contain	had	1477:1479	arg2	TB					1507:1508	culture confirmed pleural TB	1481:1508	culture confirmed pleural TB	1481:1508	Thirteen patients had culture confirmed pleural TB, 26 (81%) were HIV-TB co-infected, and 64% had < 100 CD4+ T-cells/microL.
32611401	11	112	contain	had	1477:1479	arg1	patients					1468:1475	Thirteen patients	1459:1475	Thirteen patients	1459:1475	Thirteen patients had culture confirmed pleural TB, 26 (81%) were HIV-TB co-infected, and 64% had < 100 CD4+ T-cells/microL.
32611401	6	113	theme	mycobacterial	1120:1132	arg1	antigens					1134:1141	various mycobacterial antigens	1112:1141	various mycobacterial antigens	1112:1141	Pleural fluid mononuclear cells (PFMC) were isolated, and cell smears were stained with acid-fast staining and immunocytochemistry for various mycobacterial antigens.
32611401	10	114	theme	patients	1376:1383	arg1	total					1364:1368	A total	1362:1368	A total of 41 patients	1362:1383	RESULTS A total of 41 patients were enrolled, of which 32 were classified as pleural TB and 9 as non-TB.
32611401	18	115	theme	detection	2332:2340	arg1	complement					2374:2383	a complement	2372:2383	a complement to acid-fast staining	2372:2405	Thus, a combination of secreted antigen and LAM detection by immunocytochemistry may be a complement to acid-fast staining and contribute to rapid and accurate diagnosis of pleural TB.
32611401	18	115	theme	detection	2332:2340	arg1	combination					2292:2302	a combination	2290:2302	a combination of secreted antigen and LAM detection by immunocytochemistry	2290:2363	Thus, a combination of secreted antigen and LAM detection by immunocytochemistry may be a complement to acid-fast staining and contribute to rapid and accurate diagnosis of pleural TB.
32611401	3	116	theme	diagnostic	647:656	arg1	potential					658:666	the diagnostic potential	643:666	the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB	643:725	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	1	117	theme	HIV-infected	233:244	arg1	individuals					246:256	HIV-infected individuals	233:256	HIV-infected individuals with pleural TB as the second most common location	233:307	BACKGROUND Extra pulmonary manifestation of tuberculosis (TB) accounts for approximately one-half of TB cases in HIV-infected individuals with pleural TB as the second most common location.
32611401	3	118	from	potential	658:666	arg1	presence					617:624	the presence	613:624	the presence of antigens	613:636	The aim of this study was to explore various mycobacterial antigens in pleural effusions, the impact of HIV infection and CD4+ T-cell depletion on the presence of antigens, and the diagnostic potential of antigens for improved and rapid diagnosis of pleural TB.
32611401	11	119	theme	CD4+	1563:1566	arg1	T-cells/microL					1568:1581	< 100 CD4+ T-cells/microL	1557:1581	< 100 CD4+ T-cells/microL	1557:1581	Thirteen patients had culture confirmed pleural TB, 26 (81%) were HIV-TB co-infected, and 64% had < 100 CD4+ T-cells/microL.
32843013	4	0	theme	starch	791:796	arg1	composition					798:808	their starch composition	785:808	their starch composition	785:808	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	0	theme	starch	791:796	arg1	METHODS					594:600	METHODS	594:600	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties	594:885	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	7	1	theme	beta-amyrin	1177:1187	arg1	tetrahydro					1265:1274	tetrahydro	1265:1274	tetrahydro	1265:1274	Furthermore, the fruit parts contain high concentrations of beta-amyrin acetate, eleagine, epicatechin, epigallocatechin, skatole, stigmasterol and tetrahydro - 2- methylharman as revealed by HPLC-DAD.
32843013	7	1	theme	beta-amyrin	1177:1187	arg1	acetate					1189:1195	beta-amyrin acetate	1177:1195	beta-amyrin acetate	1177:1195	Furthermore, the fruit parts contain high concentrations of beta-amyrin acetate, eleagine, epicatechin, epigallocatechin, skatole, stigmasterol and tetrahydro - 2- methylharman as revealed by HPLC-DAD.
32843013	7	1	theme	beta-amyrin	1177:1187	arg1	eleagine					1198:1205	eleagine	1198:1205	eleagine	1198:1205	Furthermore, the fruit parts contain high concentrations of beta-amyrin acetate, eleagine, epicatechin, epigallocatechin, skatole, stigmasterol and tetrahydro - 2- methylharman as revealed by HPLC-DAD.
32843013	7	1	theme	beta-amyrin	1177:1187	arg1	skatole					1239:1245	skatole	1239:1245	skatole	1239:1245	Furthermore, the fruit parts contain high concentrations of beta-amyrin acetate, eleagine, epicatechin, epigallocatechin, skatole, stigmasterol and tetrahydro - 2- methylharman as revealed by HPLC-DAD.
32843013	7	1	theme	beta-amyrin	1177:1187	arg1	stigmasterol					1248:1259	stigmasterol	1248:1259	stigmasterol	1248:1259	Furthermore, the fruit parts contain high concentrations of beta-amyrin acetate, eleagine, epicatechin, epigallocatechin, skatole, stigmasterol and tetrahydro - 2- methylharman as revealed by HPLC-DAD.
32843013	7	1	theme	beta-amyrin	1177:1187	arg1	epicatechin					1208:1218	epicatechin	1208:1218	epicatechin	1208:1218	Furthermore, the fruit parts contain high concentrations of beta-amyrin acetate, eleagine, epicatechin, epigallocatechin, skatole, stigmasterol and tetrahydro - 2- methylharman as revealed by HPLC-DAD.
32843013	7	1	theme	beta-amyrin	1177:1187	arg1	epigallocatechin					1221:1236	epigallocatechin	1221:1236	epigallocatechin	1221:1236	Furthermore, the fruit parts contain high concentrations of beta-amyrin acetate, eleagine, epicatechin, epigallocatechin, skatole, stigmasterol and tetrahydro - 2- methylharman as revealed by HPLC-DAD.
32843013	3	2	theme	apple	492:496	arg1	parts					504:508	African star apple fruit parts	479:508	African star apple fruit parts	479:508	The current study examined and compared the inhibitory abilities of African star apple fruit parts on selected key enzymes related to diabetes mellitus in the pancreas tissue of rat.
32843013	1	3	theme	tropical	245:252	arg1	regions					254:260	tropical regions	245:260	tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes	245:346	BACKGROUND African star apple (Chrysophyllum albidum) is a traditonal fruit, which is predominant in tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes.
32843013	10	4	theme	apple	1678:1682	arg1	coat					1689:1692	the African star apple pulp coat	1661:1692	the African star apple pulp coat	1661:1692	Nevertheless, the African star apple pulp coat displayed the highest property in comparison to other parts of the fruit.
32843013	3	5	theme	selected	513:520	arg1	enzymes					526:532	selected key enzymes	513:532	selected key enzymes related to diabetes mellitus in the pancreas tissue of rat	513:591	The current study examined and compared the inhibitory abilities of African star apple fruit parts on selected key enzymes related to diabetes mellitus in the pancreas tissue of rat.
32843013	9	6	theme	diabetes	1637:1644	arg1	management					1616:1625	management	1616:1625	management	1616:1625	This could also be a potential mechanism for the use in the prevention and management of type-2 diabetes.
32843013	9	6	theme	diabetes	1637:1644	arg1	prevention					1601:1610	prevention	1601:1610	prevention	1601:1610	This could also be a potential mechanism for the use in the prevention and management of type-2 diabetes.
32843013	4	7	theme	phenolic	811:818	arg1	constituents					820:831	phenolic constituents	811:831	phenolic constituents	811:831	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	0	8	theme	apple	125:129	arg1	parts					137:141	African star apple fruit parts	112:141	African star apple fruit parts	112:141	Starch composition, glycemic indices, antioxidant properties and carbohydrate hydrolyzing enzymes activities of African star apple fruit parts.
32843013	10	9	theme	star	1673:1676	arg1	coat					1689:1692	the African star apple pulp coat	1661:1692	the African star apple pulp coat	1661:1692	Nevertheless, the African star apple pulp coat displayed the highest property in comparison to other parts of the fruit.
32843013	4	10	from	effect	613:618	arg1	activities					734:743	the activities	730:743	the activities of α-amylase	730:756	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	11	theme	aqueous	623:629	arg1	1:10 w/v					640:647	1:10 w/v	640:647	1:10 w/v	640:647	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	11	theme	aqueous	623:629	arg1	extract					631:637	aqueous extract	623:637	aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat)	623:725	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	0	12	theme	parts	137:141	arg1	activities					98:107	Starch composition, glycemic indices, antioxidant properties and carbohydrate hydrolyzing enzymes activities	0:107	Starch composition, glycemic indices, antioxidant properties and carbohydrate hydrolyzing enzymes activities of African star apple fruit parts.	0:142	Starch composition, glycemic indices, antioxidant properties and carbohydrate hydrolyzing enzymes activities of African star apple fruit parts.
32843013	4	13	from	index	853:857	arg1	activities					734:743	the activities	730:743	the activities of α-amylase	730:756	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	14	from	α-glucosidase	759:771	arg1	activities					734:743	the activities	730:743	the activities of α-amylase	730:756	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	15	theme	parts	678:682	arg1	1:10 w/v					640:647	1:10 w/v	640:647	1:10 w/v	640:647	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	15	theme	parts	678:682	arg1	extract					631:637	aqueous extract	623:637	aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat)	623:725	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	1	16	from	predominant	230:240	arg1	regions					254:260	tropical regions	245:260	tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes	245:346	BACKGROUND African star apple (Chrysophyllum albidum) is a traditonal fruit, which is predominant in tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes.
32843013	4	17	dep	METHODS	594:600	arg1	index					853:857	estimated glycemic index	834:857	estimated glycemic index	834:857	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	17	dep	METHODS	594:600	arg1	effect					613:618	Inhibitory effect	602:618	Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase	602:756	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	17	dep	METHODS	594:600	arg1	METHODS					594:600	METHODS	594:600	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties	594:885	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	17	dep	METHODS	594:600	arg1	constituents					820:831	phenolic constituents	811:831	phenolic constituents	811:831	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	17	dep	METHODS	594:600	arg1	properties					876:885	antioxidant properties	864:885	antioxidant properties	864:885	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	17	dep	METHODS	594:600	arg1	α-glucosidase					759:771	α-glucosidase	759:771	α-glucosidase	759:771	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	17	dep	METHODS	594:600	arg1	composition					798:808	their starch composition	785:808	their starch composition	785:808	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	18	theme	estimated	834:842	arg1	index					853:857	estimated glycemic index	834:857	estimated glycemic index	834:857	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	18	theme	estimated	834:842	arg1	METHODS					594:600	METHODS	594:600	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties	594:885	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	8	19	theme	α-amylase	1422:1430	arg1	activities					1450:1459	α-amylase and α-glucosidase activities	1422:1459	α-amylase and α-glucosidase activities exhibited might be related to the bioactive compounds contained in the extract	1422:1538	CONCLUSION The fruit part low estimated glycemic indices, strong antioxidant properties, inhibition of α-amylase and α-glucosidase activities exhibited might be related to the bioactive compounds contained in the extract.
32843013	8	20	theme	fruit	1334:1338	arg1	part					1340:1343	The fruit part low	1330:1347	The fruit part low	1330:1347	CONCLUSION The fruit part low estimated glycemic indices, strong antioxidant properties, inhibition of α-amylase and α-glucosidase activities exhibited might be related to the bioactive compounds contained in the extract.
32843013	5	21	theme	amylopectin	962:972	arg1	contents					974:981	amylopectin contents	962:981	amylopectin contents	962:981	RESULTS The fruit parts showed low sugar, eGI, amylose, and amylopectin contents.
32843013	8	22	theme	α-glucosidase	1436:1448	arg1	activities					1450:1459	α-amylase and α-glucosidase activities	1422:1459	α-amylase and α-glucosidase activities exhibited might be related to the bioactive compounds contained in the extract	1422:1538	CONCLUSION The fruit part low estimated glycemic indices, strong antioxidant properties, inhibition of α-amylase and α-glucosidase activities exhibited might be related to the bioactive compounds contained in the extract.
32843013	4	23	theme	African	653:659	arg1	parts					678:682	African star apple fruit parts	653:682	African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat)	653:725	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	23	theme	African	653:659	arg1	coat					721:724	pulp coat	716:724	pulp coat	716:724	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	23	theme	African	653:659	arg1	cotyledon					691:699	cotyledon	691:699	cotyledon	691:699	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	23	theme	African	653:659	arg1	coat					707:710	seed coat	702:710	seed coat	702:710	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	23	theme	African	653:659	arg1	pulp					685:688	pulp	685:688	pulp	685:688	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	3	24	from	mellitus	554:561	arg1	tissue					579:584	the pancreas tissue	566:584	the pancreas tissue of rat	566:591	The current study examined and compared the inhibitory abilities of African star apple fruit parts on selected key enzymes related to diabetes mellitus in the pancreas tissue of rat.
32843013	3	25	theme	rat	589:591	arg1	tissue					579:584	the pancreas tissue	566:584	the pancreas tissue of rat	566:591	The current study examined and compared the inhibitory abilities of African star apple fruit parts on selected key enzymes related to diabetes mellitus in the pancreas tissue of rat.
32843013	1	26	theme	African	155:161	arg1	fruit					214:218	a traditonal fruit	201:218	a traditonal fruit	201:218	BACKGROUND African star apple (Chrysophyllum albidum) is a traditonal fruit, which is predominant in tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes.
32843013	1	26	theme	African	155:161	arg1	predominant					230:240	predominant	230:240	predominant	230:240	BACKGROUND African star apple (Chrysophyllum albidum) is a traditonal fruit, which is predominant in tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes.
32843013	1	26	theme	African	155:161	arg1	apple					168:172	BACKGROUND African star apple	144:172	BACKGROUND African star apple (Chrysophyllum albidum)	144:196	BACKGROUND African star apple (Chrysophyllum albidum) is a traditonal fruit, which is predominant in tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes.
32843013	4	27	theme	apple	666:670	arg1	parts					678:682	African star apple fruit parts	653:682	African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat)	653:725	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	27	theme	apple	666:670	arg1	coat					721:724	pulp coat	716:724	pulp coat	716:724	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	27	theme	apple	666:670	arg1	cotyledon					691:699	cotyledon	691:699	cotyledon	691:699	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	27	theme	apple	666:670	arg1	coat					707:710	seed coat	702:710	seed coat	702:710	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	27	theme	apple	666:670	arg1	pulp					685:688	pulp	685:688	pulp	685:688	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	0	28	theme	hydrolyzing	78:88	arg1	enzymes					90:96	carbohydrate hydrolyzing enzymes	65:96	carbohydrate hydrolyzing enzymes	65:96	Starch composition, glycemic indices, antioxidant properties and carbohydrate hydrolyzing enzymes activities of African star apple fruit parts.
32843013	4	29	from	constituents	820:831	arg1	activities					734:743	the activities	730:743	the activities of α-amylase	730:756	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	10	30	theme	highest	1708:1714	arg1	property					1716:1723	the highest property	1704:1723	the highest property	1704:1723	Nevertheless, the African star apple pulp coat displayed the highest property in comparison to other parts of the fruit.
32843013	7	31	dep	epicatechin	1208:1218	arg1	methylharman					1281:1292	- 2- methylharman	1276:1292	- 2- methylharman	1276:1292	Furthermore, the fruit parts contain high concentrations of beta-amyrin acetate, eleagine, epicatechin, epigallocatechin, skatole, stigmasterol and tetrahydro - 2- methylharman as revealed by HPLC-DAD.
32843013	9	32	from	use	1590:1592	arg1	management					1616:1625	management	1616:1625	management	1616:1625	This could also be a potential mechanism for the use in the prevention and management of type-2 diabetes.
32843013	9	32	from	use	1590:1592	arg1	prevention					1601:1610	prevention	1601:1610	prevention	1601:1610	This could also be a potential mechanism for the use in the prevention and management of type-2 diabetes.
32843013	2	33	theme	activity	369:376	arg1	mechanism					378:386	the likely activity mechanism	358:386	the likely activity mechanism	358:386	However, the likely activity mechanism is still undetermined.
32843013	0	34	theme	African	112:118	arg1	parts					137:141	African star apple fruit parts	112:141	African star apple fruit parts	112:141	Starch composition, glycemic indices, antioxidant properties and carbohydrate hydrolyzing enzymes activities of African star apple fruit parts.
32843013	5	35	theme	fruit	914:918	arg1	parts					920:924	The fruit parts	910:924	RESULTS The fruit parts	902:924	RESULTS The fruit parts showed low sugar, eGI, amylose, and amylopectin contents.
32843013	8	36	theme	bioactive	1495:1503	arg1	compounds					1505:1513	the bioactive compounds	1491:1513	the bioactive compounds contained in the extract	1491:1538	CONCLUSION The fruit part low estimated glycemic indices, strong antioxidant properties, inhibition of α-amylase and α-glucosidase activities exhibited might be related to the bioactive compounds contained in the extract.
32843013	0	37	theme	Starch	0:5	arg1	composition					7:17	Starch composition	0:17	Starch composition	0:17	Starch composition, glycemic indices, antioxidant properties and carbohydrate hydrolyzing enzymes activities of African star apple fruit parts.
32843013	4	38	theme	seed	702:705	arg1	parts					678:682	African star apple fruit parts	653:682	African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat)	653:725	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	38	theme	seed	702:705	arg1	coat					707:710	seed coat	702:710	seed coat	702:710	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	8	39	theme	low	1345:1347	arg1	part					1340:1343	The fruit part low	1330:1347	The fruit part low	1330:1347	CONCLUSION The fruit part low estimated glycemic indices, strong antioxidant properties, inhibition of α-amylase and α-glucosidase activities exhibited might be related to the bioactive compounds contained in the extract.
32843013	9	40	dep	prevention	1601:1610	arg1	the					1597:1599	the	1597:1599	the	1597:1599	This could also be a potential mechanism for the use in the prevention and management of type-2 diabetes.
32843013	4	41	theme	α-amylase	748:756	arg1	activities					734:743	the activities	730:743	the activities of α-amylase	730:756	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	0	42	theme	indices	29:35	arg1	activities					98:107	Starch composition, glycemic indices, antioxidant properties and carbohydrate hydrolyzing enzymes activities	0:107	Starch composition, glycemic indices, antioxidant properties and carbohydrate hydrolyzing enzymes activities of African star apple fruit parts.	0:142	Starch composition, glycemic indices, antioxidant properties and carbohydrate hydrolyzing enzymes activities of African star apple fruit parts.
32843013	1	43	with	regions	254:260	arg1	parts					277:281	the fruit parts	267:281	the fruit parts consumed by the populace and used in folklore to manage diabetes	267:346	BACKGROUND African star apple (Chrysophyllum albidum) is a traditonal fruit, which is predominant in tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes.
32843013	8	44	theme	glycemic	1359:1366	arg1	inhibition					1408:1417	inhibition	1408:1417	inhibition of α-amylase and α-glucosidase activities exhibited might be related to the bioactive compounds contained in the extract	1408:1538	CONCLUSION The fruit part low estimated glycemic indices, strong antioxidant properties, inhibition of α-amylase and α-glucosidase activities exhibited might be related to the bioactive compounds contained in the extract.
32843013	8	44	theme	glycemic	1359:1366	arg1	properties					1396:1405	strong antioxidant properties	1377:1405	strong antioxidant properties	1377:1405	CONCLUSION The fruit part low estimated glycemic indices, strong antioxidant properties, inhibition of α-amylase and α-glucosidase activities exhibited might be related to the bioactive compounds contained in the extract.
32843013	8	44	theme	glycemic	1359:1366	arg1	indices					1368:1374	glycemic indices	1359:1374	glycemic indices	1359:1374	CONCLUSION The fruit part low estimated glycemic indices, strong antioxidant properties, inhibition of α-amylase and α-glucosidase activities exhibited might be related to the bioactive compounds contained in the extract.
32843013	10	45	theme	African	1665:1671	arg1	coat					1689:1692	the African star apple pulp coat	1661:1692	the African star apple pulp coat	1661:1692	Nevertheless, the African star apple pulp coat displayed the highest property in comparison to other parts of the fruit.
32843013	0	46	theme	antioxidant	38:48	arg1	properties					50:59	antioxidant properties	38:59	antioxidant properties	38:59	Starch composition, glycemic indices, antioxidant properties and carbohydrate hydrolyzing enzymes activities of African star apple fruit parts.
32843013	6	47	theme	α-glucosidase	1040:1052	arg1	activities					1068:1077	α-glucosidase and α-amylase activities	1040:1077	α-glucosidase and α-amylase activities	1040:1077	The analysis also showed that the fruit parts inhibited α-glucosidase and α-amylase activities and exhibited antioxidant properties.
32843013	3	48	theme	inhibitory	455:464	arg1	abilities					466:474	the inhibitory abilities	451:474	the inhibitory abilities of African star apple fruit parts on selected key enzymes related to diabetes mellitus in the pancreas tissue of rat	451:591	The current study examined and compared the inhibitory abilities of African star apple fruit parts on selected key enzymes related to diabetes mellitus in the pancreas tissue of rat.
32843013	8	49	theme	antioxidant	1384:1394	arg1	properties					1396:1405	strong antioxidant properties	1377:1405	strong antioxidant properties	1377:1405	CONCLUSION The fruit part low estimated glycemic indices, strong antioxidant properties, inhibition of α-amylase and α-glucosidase activities exhibited might be related to the bioactive compounds contained in the extract.
32843013	8	49	theme	antioxidant	1384:1394	arg1	indices					1368:1374	glycemic indices	1359:1374	glycemic indices	1359:1374	CONCLUSION The fruit part low estimated glycemic indices, strong antioxidant properties, inhibition of α-amylase and α-glucosidase activities exhibited might be related to the bioactive compounds contained in the extract.
32843013	7	50	contain	contain	1146:1152	arg2	concentrations					1159:1172	high concentrations	1154:1172	high concentrations of beta-amyrin acetate, eleagine, epicatechin, epigallocatechin, skatole, stigmasterol and tetrahydro - 2- methylharman	1154:1292	Furthermore, the fruit parts contain high concentrations of beta-amyrin acetate, eleagine, epicatechin, epigallocatechin, skatole, stigmasterol and tetrahydro - 2- methylharman as revealed by HPLC-DAD.
32843013	7	50	contain	contain	1146:1152	arg1	parts					1140:1144	the fruit parts	1130:1144	the fruit parts	1130:1144	Furthermore, the fruit parts contain high concentrations of beta-amyrin acetate, eleagine, epicatechin, epigallocatechin, skatole, stigmasterol and tetrahydro - 2- methylharman as revealed by HPLC-DAD.
32843013	3	51	theme	fruit	498:502	arg1	parts					504:508	African star apple fruit parts	479:508	African star apple fruit parts	479:508	The current study examined and compared the inhibitory abilities of African star apple fruit parts on selected key enzymes related to diabetes mellitus in the pancreas tissue of rat.
32843013	3	52	theme	star	487:490	arg1	parts					504:508	African star apple fruit parts	479:508	African star apple fruit parts	479:508	The current study examined and compared the inhibitory abilities of African star apple fruit parts on selected key enzymes related to diabetes mellitus in the pancreas tissue of rat.
32843013	7	53	theme	acetate	1189:1195	arg1	concentrations					1159:1172	high concentrations	1154:1172	high concentrations of beta-amyrin acetate, eleagine, epicatechin, epigallocatechin, skatole, stigmasterol and tetrahydro - 2- methylharman	1154:1292	Furthermore, the fruit parts contain high concentrations of beta-amyrin acetate, eleagine, epicatechin, epigallocatechin, skatole, stigmasterol and tetrahydro - 2- methylharman as revealed by HPLC-DAD.
32843013	3	54	theme	key	522:524	arg1	enzymes					526:532	selected key enzymes	513:532	selected key enzymes related to diabetes mellitus in the pancreas tissue of rat	513:591	The current study examined and compared the inhibitory abilities of African star apple fruit parts on selected key enzymes related to diabetes mellitus in the pancreas tissue of rat.
32843013	4	55	from	properties	876:885	arg1	activities					734:743	the activities	730:743	the activities of α-amylase	730:756	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	10	56	theme	other	1742:1746	arg1	parts					1748:1752	other parts	1742:1752	other parts of the fruit	1742:1765	Nevertheless, the African star apple pulp coat displayed the highest property in comparison to other parts of the fruit.
32843013	9	57	theme	type-2	1630:1635	arg1	diabetes					1637:1644	type-2 diabetes	1630:1644	type-2 diabetes	1630:1644	This could also be a potential mechanism for the use in the prevention and management of type-2 diabetes.
32843013	6	58	theme	antioxidant	1093:1103	arg1	properties					1105:1114	antioxidant properties	1093:1114	antioxidant properties	1093:1114	The analysis also showed that the fruit parts inhibited α-glucosidase and α-amylase activities and exhibited antioxidant properties.
32843013	4	59	theme	extract	631:637	arg1	index					853:857	estimated glycemic index	834:857	estimated glycemic index	834:857	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	59	theme	extract	631:637	arg1	effect					613:618	Inhibitory effect	602:618	Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase	602:756	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	59	theme	extract	631:637	arg1	METHODS					594:600	METHODS	594:600	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties	594:885	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	59	theme	extract	631:637	arg1	constituents					820:831	phenolic constituents	811:831	phenolic constituents	811:831	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	59	theme	extract	631:637	arg1	properties					876:885	antioxidant properties	864:885	antioxidant properties	864:885	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	59	theme	extract	631:637	arg1	α-glucosidase					759:771	α-glucosidase	759:771	α-glucosidase	759:771	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	59	theme	extract	631:637	arg1	composition					798:808	their starch composition	785:808	their starch composition	785:808	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	7	60	theme	high	1154:1157	arg1	concentrations					1159:1172	high concentrations	1154:1172	high concentrations of beta-amyrin acetate, eleagine, epicatechin, epigallocatechin, skatole, stigmasterol and tetrahydro - 2- methylharman	1154:1292	Furthermore, the fruit parts contain high concentrations of beta-amyrin acetate, eleagine, epicatechin, epigallocatechin, skatole, stigmasterol and tetrahydro - 2- methylharman as revealed by HPLC-DAD.
32843013	3	61	from	abilities	466:474	arg1	enzymes					526:532	selected key enzymes	513:532	selected key enzymes related to diabetes mellitus in the pancreas tissue of rat	513:591	The current study examined and compared the inhibitory abilities of African star apple fruit parts on selected key enzymes related to diabetes mellitus in the pancreas tissue of rat.
32843013	3	62	theme	diabetes	545:552	arg1	mellitus					554:561	diabetes mellitus	545:561	diabetes mellitus in the pancreas tissue of rat	545:591	The current study examined and compared the inhibitory abilities of African star apple fruit parts on selected key enzymes related to diabetes mellitus in the pancreas tissue of rat.
32843013	8	63	dep	CONCLUSION	1319:1328	arg1	estimated					1349:1357	estimated	1349:1357	estimated glycemic indices, strong antioxidant properties, inhibition of α-amylase and α-glucosidase activities exhibited might be related to the bioactive compounds contained in the extract	1349:1538	CONCLUSION The fruit part low estimated glycemic indices, strong antioxidant properties, inhibition of α-amylase and α-glucosidase activities exhibited might be related to the bioactive compounds contained in the extract.
32843013	1	64	from	regions	254:260	arg1	predominant					230:240	predominant	230:240	predominant	230:240	BACKGROUND African star apple (Chrysophyllum albidum) is a traditonal fruit, which is predominant in tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes.
32843013	1	64	from	regions	254:260	arg1	apple					168:172	BACKGROUND African star apple	144:172	BACKGROUND African star apple (Chrysophyllum albidum)	144:196	BACKGROUND African star apple (Chrysophyllum albidum) is a traditonal fruit, which is predominant in tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes.
32843013	1	64	from	regions	254:260	arg1	fruit					214:218	a traditonal fruit	201:218	a traditonal fruit	201:218	BACKGROUND African star apple (Chrysophyllum albidum) is a traditonal fruit, which is predominant in tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes.
32843013	3	65	theme	related	534:540	arg1	enzymes					526:532	selected key enzymes	513:532	selected key enzymes related to diabetes mellitus in the pancreas tissue of rat	513:591	The current study examined and compared the inhibitory abilities of African star apple fruit parts on selected key enzymes related to diabetes mellitus in the pancreas tissue of rat.
32843013	4	66	theme	glycemic	844:851	arg1	index					853:857	estimated glycemic index	834:857	estimated glycemic index	834:857	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	66	theme	glycemic	844:851	arg1	METHODS					594:600	METHODS	594:600	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties	594:885	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	6	67	theme	α-amylase	1058:1066	arg1	activities					1068:1077	α-glucosidase and α-amylase activities	1040:1077	α-glucosidase and α-amylase activities	1040:1077	The analysis also showed that the fruit parts inhibited α-glucosidase and α-amylase activities and exhibited antioxidant properties.
32843013	4	68	theme	Inhibitory	602:611	arg1	effect					613:618	Inhibitory effect	602:618	Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase	602:756	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	68	theme	Inhibitory	602:611	arg1	METHODS					594:600	METHODS	594:600	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties	594:885	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	3	69	theme	pancreas	570:577	arg1	tissue					579:584	the pancreas tissue	566:584	the pancreas tissue of rat	566:591	The current study examined and compared the inhibitory abilities of African star apple fruit parts on selected key enzymes related to diabetes mellitus in the pancreas tissue of rat.
32843013	1	70	theme	traditonal	203:212	arg1	predominant					230:240	predominant	230:240	predominant	230:240	BACKGROUND African star apple (Chrysophyllum albidum) is a traditonal fruit, which is predominant in tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes.
32843013	1	70	theme	traditonal	203:212	arg1	apple					168:172	BACKGROUND African star apple	144:172	BACKGROUND African star apple (Chrysophyllum albidum)	144:196	BACKGROUND African star apple (Chrysophyllum albidum) is a traditonal fruit, which is predominant in tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes.
32843013	1	70	theme	traditonal	203:212	arg1	fruit					214:218	a traditonal fruit	201:218	a traditonal fruit	201:218	BACKGROUND African star apple (Chrysophyllum albidum) is a traditonal fruit, which is predominant in tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes.
32843013	1	71	attach	predominant	230:240	arg2	fruit					214:218	a traditonal fruit	201:218	a traditonal fruit	201:218	BACKGROUND African star apple (Chrysophyllum albidum) is a traditonal fruit, which is predominant in tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes.
32843013	1	71	attach	predominant	230:240	arg2	predominant					230:240	predominant	230:240	predominant	230:240	BACKGROUND African star apple (Chrysophyllum albidum) is a traditonal fruit, which is predominant in tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes.
32843013	1	71	attach	predominant	230:240	arg1	regions					254:260	tropical regions	245:260	tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes	245:346	BACKGROUND African star apple (Chrysophyllum albidum) is a traditonal fruit, which is predominant in tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes.
32843013	1	71	attach	predominant	230:240	arg2	apple					168:172	BACKGROUND African star apple	144:172	BACKGROUND African star apple (Chrysophyllum albidum)	144:196	BACKGROUND African star apple (Chrysophyllum albidum) is a traditonal fruit, which is predominant in tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes.
32843013	0	72	theme	fruit	131:135	arg1	parts					137:141	African star apple fruit parts	112:141	African star apple fruit parts	112:141	Starch composition, glycemic indices, antioxidant properties and carbohydrate hydrolyzing enzymes activities of African star apple fruit parts.
32843013	4	73	theme	fruit	672:676	arg1	parts					678:682	African star apple fruit parts	653:682	African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat)	653:725	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	73	theme	fruit	672:676	arg1	coat					721:724	pulp coat	716:724	pulp coat	716:724	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	73	theme	fruit	672:676	arg1	cotyledon					691:699	cotyledon	691:699	cotyledon	691:699	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	73	theme	fruit	672:676	arg1	coat					707:710	seed coat	702:710	seed coat	702:710	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	73	theme	fruit	672:676	arg1	pulp					685:688	pulp	685:688	pulp	685:688	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	8	74	theme	activities	1450:1459	arg1	inhibition					1408:1417	inhibition	1408:1417	inhibition of α-amylase and α-glucosidase activities exhibited might be related to the bioactive compounds contained in the extract	1408:1538	CONCLUSION The fruit part low estimated glycemic indices, strong antioxidant properties, inhibition of α-amylase and α-glucosidase activities exhibited might be related to the bioactive compounds contained in the extract.
32843013	8	74	theme	activities	1450:1459	arg1	indices					1368:1374	glycemic indices	1359:1374	glycemic indices	1359:1374	CONCLUSION The fruit part low estimated glycemic indices, strong antioxidant properties, inhibition of α-amylase and α-glucosidase activities exhibited might be related to the bioactive compounds contained in the extract.
32843013	5	75	dep	RESULTS	902:908	arg1	parts					920:924	The fruit parts	910:924	RESULTS The fruit parts	902:924	RESULTS The fruit parts showed low sugar, eGI, amylose, and amylopectin contents.
32843013	0	76	theme	carbohydrate	65:76	arg1	enzymes					90:96	carbohydrate hydrolyzing enzymes	65:96	carbohydrate hydrolyzing enzymes	65:96	Starch composition, glycemic indices, antioxidant properties and carbohydrate hydrolyzing enzymes activities of African star apple fruit parts.
32843013	1	77	theme	BACKGROUND	144:153	arg1	fruit					214:218	a traditonal fruit	201:218	a traditonal fruit	201:218	BACKGROUND African star apple (Chrysophyllum albidum) is a traditonal fruit, which is predominant in tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes.
32843013	1	77	theme	BACKGROUND	144:153	arg1	predominant					230:240	predominant	230:240	predominant	230:240	BACKGROUND African star apple (Chrysophyllum albidum) is a traditonal fruit, which is predominant in tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes.
32843013	1	77	theme	BACKGROUND	144:153	arg1	apple					168:172	BACKGROUND African star apple	144:172	BACKGROUND African star apple (Chrysophyllum albidum)	144:196	BACKGROUND African star apple (Chrysophyllum albidum) is a traditonal fruit, which is predominant in tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes.
32843013	1	78	dep	parts	277:281	arg1	used					312:315	used	312:315	used in folklore to manage diabetes	312:346	BACKGROUND African star apple (Chrysophyllum albidum) is a traditonal fruit, which is predominant in tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes.
32843013	1	78	dep	parts	277:281	arg1	consumed					283:290	consumed	283:290	consumed by the populace	283:306	BACKGROUND African star apple (Chrysophyllum albidum) is a traditonal fruit, which is predominant in tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes.
32843013	0	79	theme	enzymes	90:96	arg1	activities					98:107	Starch composition, glycemic indices, antioxidant properties and carbohydrate hydrolyzing enzymes activities	0:107	Starch composition, glycemic indices, antioxidant properties and carbohydrate hydrolyzing enzymes activities of African star apple fruit parts.	0:142	Starch composition, glycemic indices, antioxidant properties and carbohydrate hydrolyzing enzymes activities of African star apple fruit parts.
32843013	4	80	from	composition	798:808	arg1	activities					734:743	the activities	730:743	the activities of α-amylase	730:756	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	1	81	theme	star	163:166	arg1	fruit					214:218	a traditonal fruit	201:218	a traditonal fruit	201:218	BACKGROUND African star apple (Chrysophyllum albidum) is a traditonal fruit, which is predominant in tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes.
32843013	1	81	theme	star	163:166	arg1	predominant					230:240	predominant	230:240	predominant	230:240	BACKGROUND African star apple (Chrysophyllum albidum) is a traditonal fruit, which is predominant in tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes.
32843013	1	81	theme	star	163:166	arg1	apple					168:172	BACKGROUND African star apple	144:172	BACKGROUND African star apple (Chrysophyllum albidum)	144:196	BACKGROUND African star apple (Chrysophyllum albidum) is a traditonal fruit, which is predominant in tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes.
32843013	4	82	theme	star	661:664	arg1	parts					678:682	African star apple fruit parts	653:682	African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat)	653:725	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	82	theme	star	661:664	arg1	coat					721:724	pulp coat	716:724	pulp coat	716:724	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	82	theme	star	661:664	arg1	cotyledon					691:699	cotyledon	691:699	cotyledon	691:699	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	82	theme	star	661:664	arg1	coat					707:710	seed coat	702:710	seed coat	702:710	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	82	theme	star	661:664	arg1	pulp					685:688	pulp	685:688	pulp	685:688	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	7	83	theme	fruit	1134:1138	arg1	parts					1140:1144	the fruit parts	1130:1144	the fruit parts	1130:1144	Furthermore, the fruit parts contain high concentrations of beta-amyrin acetate, eleagine, epicatechin, epigallocatechin, skatole, stigmasterol and tetrahydro - 2- methylharman as revealed by HPLC-DAD.
32843013	4	84	theme	antioxidant	864:874	arg1	properties					876:885	antioxidant properties	864:885	antioxidant properties	864:885	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	0	85	theme	star	120:123	arg1	parts					137:141	African star apple fruit parts	112:141	African star apple fruit parts	112:141	Starch composition, glycemic indices, antioxidant properties and carbohydrate hydrolyzing enzymes activities of African star apple fruit parts.
32843013	10	86	dep	parts	1748:1752	arg1	comparison					1728:1737	comparison	1728:1737	comparison	1728:1737	Nevertheless, the African star apple pulp coat displayed the highest property in comparison to other parts of the fruit.
32843013	7	87	theme	- 2-	1276:1279	arg1	methylharman					1281:1292	- 2- methylharman	1276:1292	- 2- methylharman	1276:1292	Furthermore, the fruit parts contain high concentrations of beta-amyrin acetate, eleagine, epicatechin, epigallocatechin, skatole, stigmasterol and tetrahydro - 2- methylharman as revealed by HPLC-DAD.
32843013	4	88	theme	pulp	716:719	arg1	parts					678:682	African star apple fruit parts	653:682	African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat)	653:725	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	88	theme	pulp	716:719	arg1	coat					721:724	pulp coat	716:724	pulp coat	716:724	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	2	89	theme	likely	362:367	arg1	mechanism					378:386	the likely activity mechanism	358:386	the likely activity mechanism	358:386	However, the likely activity mechanism is still undetermined.
32843013	0	90	theme	composition	7:17	arg1	activities					98:107	Starch composition, glycemic indices, antioxidant properties and carbohydrate hydrolyzing enzymes activities	0:107	Starch composition, glycemic indices, antioxidant properties and carbohydrate hydrolyzing enzymes activities of African star apple fruit parts.	0:142	Starch composition, glycemic indices, antioxidant properties and carbohydrate hydrolyzing enzymes activities of African star apple fruit parts.
32843013	1	91	dep	apple	168:172	arg1	albidum					189:195	Chrysophyllum albidum	175:195	Chrysophyllum albidum	175:195	BACKGROUND African star apple (Chrysophyllum albidum) is a traditonal fruit, which is predominant in tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes.
32843013	0	92	theme	glycemic	20:27	arg1	indices					29:35	glycemic indices	20:35	glycemic indices	20:35	Starch composition, glycemic indices, antioxidant properties and carbohydrate hydrolyzing enzymes activities of African star apple fruit parts.
32843013	1	93	theme	fruit	271:275	arg1	parts					277:281	the fruit parts	267:281	the fruit parts consumed by the populace and used in folklore to manage diabetes	267:346	BACKGROUND African star apple (Chrysophyllum albidum) is a traditonal fruit, which is predominant in tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes.
32843013	4	94	dep	parts	678:682	arg1	parts					678:682	African star apple fruit parts	653:682	African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat)	653:725	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	94	dep	parts	678:682	arg1	coat					721:724	pulp coat	716:724	pulp coat	716:724	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	94	dep	parts	678:682	arg1	cotyledon					691:699	cotyledon	691:699	cotyledon	691:699	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	94	dep	parts	678:682	arg1	coat					707:710	seed coat	702:710	seed coat	702:710	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	4	94	dep	parts	678:682	arg1	pulp					685:688	pulp	685:688	pulp	685:688	METHODS Inhibitory effect of aqueous extract (1:10 w/v) of African star apple fruit parts (pulp, cotyledon, seed coat and pulp coat) on the activities of α-amylase, α-glucosidase, as well as their starch composition, phenolic constituents, estimated glycemic index, and antioxidant properties were assessed.
32843013	10	95	theme	fruit	1761:1765	arg1	parts					1748:1752	other parts	1742:1752	other parts of the fruit	1742:1765	Nevertheless, the African star apple pulp coat displayed the highest property in comparison to other parts of the fruit.
32843013	3	96	theme	current	415:421	arg1	study					423:427	The current study	411:427	The current study	411:427	The current study examined and compared the inhibitory abilities of African star apple fruit parts on selected key enzymes related to diabetes mellitus in the pancreas tissue of rat.
32843013	0	97	theme	properties	50:59	arg1	activities					98:107	Starch composition, glycemic indices, antioxidant properties and carbohydrate hydrolyzing enzymes activities	0:107	Starch composition, glycemic indices, antioxidant properties and carbohydrate hydrolyzing enzymes activities of African star apple fruit parts.	0:142	Starch composition, glycemic indices, antioxidant properties and carbohydrate hydrolyzing enzymes activities of African star apple fruit parts.
32843013	9	98	theme	potential	1562:1570	arg1	This					1541:1544	This	1541:1544	This	1541:1544	This could also be a potential mechanism for the use in the prevention and management of type-2 diabetes.
32843013	9	98	theme	potential	1562:1570	arg1	mechanism					1572:1580	a potential mechanism	1560:1580	a potential mechanism for the use in the prevention and management of type-2 diabetes	1560:1644	This could also be a potential mechanism for the use in the prevention and management of type-2 diabetes.
32843013	1	99	dep	manage	332:337	arg1	folklore					320:327	folklore	320:327	folklore	320:327	BACKGROUND African star apple (Chrysophyllum albidum) is a traditonal fruit, which is predominant in tropical regions with the fruit parts consumed by the populace and used in folklore to manage diabetes.
32843013	3	100	theme	African	479:485	arg1	parts					504:508	African star apple fruit parts	479:508	African star apple fruit parts	479:508	The current study examined and compared the inhibitory abilities of African star apple fruit parts on selected key enzymes related to diabetes mellitus in the pancreas tissue of rat.
32843013	10	101	theme	pulp	1684:1687	arg1	coat					1689:1692	the African star apple pulp coat	1661:1692	the African star apple pulp coat	1661:1692	Nevertheless, the African star apple pulp coat displayed the highest property in comparison to other parts of the fruit.
32843013	5	102	theme	low	933:935	arg1	sugar					937:941	low sugar	933:941	low sugar	933:941	RESULTS The fruit parts showed low sugar, eGI, amylose, and amylopectin contents.
32843013	6	103	theme	fruit	1018:1022	arg1	parts					1024:1028	the fruit parts	1014:1028	the fruit parts	1014:1028	The analysis also showed that the fruit parts inhibited α-glucosidase and α-amylase activities and exhibited antioxidant properties.
32843013	8	104	theme	strong	1377:1382	arg1	properties					1396:1405	strong antioxidant properties	1377:1405	strong antioxidant properties	1377:1405	CONCLUSION The fruit part low estimated glycemic indices, strong antioxidant properties, inhibition of α-amylase and α-glucosidase activities exhibited might be related to the bioactive compounds contained in the extract.
32843013	8	104	theme	strong	1377:1382	arg1	indices					1368:1374	glycemic indices	1359:1374	glycemic indices	1359:1374	CONCLUSION The fruit part low estimated glycemic indices, strong antioxidant properties, inhibition of α-amylase and α-glucosidase activities exhibited might be related to the bioactive compounds contained in the extract.
32843013	3	105	theme	parts	504:508	arg1	abilities					466:474	the inhibitory abilities	451:474	the inhibitory abilities of African star apple fruit parts on selected key enzymes related to diabetes mellitus in the pancreas tissue of rat	451:591	The current study examined and compared the inhibitory abilities of African star apple fruit parts on selected key enzymes related to diabetes mellitus in the pancreas tissue of rat.
32056374	5	0	with	resistance	961:970	arg1	concentration					890:902	GnP concentration	886:902	GnP concentration	886:902	Overall, our hydrogels exhibited (a) increasing stiffness with GnP concentration for every pre-functionalization and (b) efficient enzyme resistance with PLL treatment, and also with type IV collagen but to a lesser extent.
32056374	8	1	theme	various	1455:1461	arg1	cells					1470:1474	various cancer cells	1455:1474	various cancer cells	1455:1474	The as-desired HA-based 3D tumor-like models we developed may provide a useful platform for the study of various cancer cells by simply tuning their biochemical composition and their mechanical properties.
32056374	5	2	with	stiffness	871:879	arg1	concentration					890:902	GnP concentration	886:902	GnP concentration	886:902	Overall, our hydrogels exhibited (a) increasing stiffness with GnP concentration for every pre-functionalization and (b) efficient enzyme resistance with PLL treatment, and also with type IV collagen but to a lesser extent.
32056374	7	3	theme	pre-treated	1277:1287	arg1	hydrogels					1289:1297	type IV collagen pre-treated hydrogels	1260:1297	type IV collagen pre-treated hydrogels	1260:1297	Contrary to type III collagen, type IV collagen pre-treated hydrogels supported the proliferation of glioblastoma cells.
32056374	7	4	theme	glioblastoma	1330:1341	arg1	cells					1343:1347	glioblastoma cells	1330:1347	glioblastoma cells	1330:1347	Contrary to type III collagen, type IV collagen pre-treated hydrogels supported the proliferation of glioblastoma cells.
32056374	0	5	with	stiffness	75:83	arg1	genipin-crosslinking					90:109	genipin-crosslinking	90:109	genipin-crosslinking	90:109	Hyaluronan-based hydrogels as versatile tumor-like models: Tunable ECM and stiffness with genipin-crosslinking.
32056374	7	6	theme	type	1260:1263	arg1	collagen					1268:1275	type IV collagen	1260:1275	type IV collagen pre-treated hydrogels	1260:1297	Contrary to type III collagen, type IV collagen pre-treated hydrogels supported the proliferation of glioblastoma cells.
32056374	6	7	theme	cells	1190:1194	arg1	proliferation					1159:1171	the proliferation	1155:1171	the proliferation of breast cancer cells	1155:1194	While PLL-treated hydrogels were not favorable to the culture of any glioblastoma cell lines, they enhanced the proliferation of breast cancer cells in a stiffness-dependent manner.
32056374	5	8	dep	increasing	860:869	arg1	a					857:857	a	857:857	a	857:857	Overall, our hydrogels exhibited (a) increasing stiffness with GnP concentration for every pre-functionalization and (b) efficient enzyme resistance with PLL treatment, and also with type IV collagen but to a lesser extent.
32056374	6	9	theme	lines	1134:1138	arg1	culture					1101:1107	the culture	1097:1107	the culture of any glioblastoma cell lines	1097:1138	While PLL-treated hydrogels were not favorable to the culture of any glioblastoma cell lines, they enhanced the proliferation of breast cancer cells in a stiffness-dependent manner.
32056374	3	10	dep	proteins	493:500	arg1	collagens					524:532	type III and type IV collagens	503:532	collagens	524:532	For that, we pre-functionalized our hydrogels with an adhesive polypeptide (poly-l-lysine, PLL) or ECM proteins (type III and type IV collagens), naturally present in tumorous tissues, and next, we tuned their stiffness by crosslinking with gradual concentrations of genipin (GnP).
32056374	3	10	dep	proteins	493:500	arg1	type					503:506	type III and type IV collagens	503:532	type	503:506	For that, we pre-functionalized our hydrogels with an adhesive polypeptide (poly-l-lysine, PLL) or ECM proteins (type III and type IV collagens), naturally present in tumorous tissues, and next, we tuned their stiffness by crosslinking with gradual concentrations of genipin (GnP).
32056374	1	11	theme	cell	146:149	arg1	platforms					159:167	Three-dimensional (3D) biomimetic cell culture platforms	112:167	Three-dimensional (3D) biomimetic cell culture platforms	112:167	Three-dimensional (3D) biomimetic cell culture platforms offer more realistic microenvironments that cells naturally experience in vivo.
32056374	0	12	with	ECM	67:69	arg1	genipin-crosslinking					90:109	genipin-crosslinking	90:109	genipin-crosslinking	90:109	Hyaluronan-based hydrogels as versatile tumor-like models: Tunable ECM and stiffness with genipin-crosslinking.
32056374	5	13	theme	efficient	944:952	arg1	resistance					961:970	(b) efficient enzyme resistance	940:970	(b) efficient enzyme resistance	940:970	Overall, our hydrogels exhibited (a) increasing stiffness with GnP concentration for every pre-functionalization and (b) efficient enzyme resistance with PLL treatment, and also with type IV collagen but to a lesser extent.
32056374	1	14	theme	realistic	180:188	arg1	microenvironments					190:206	more realistic microenvironments	175:206	more realistic microenvironments that cells naturally experience in vivo	175:246	Three-dimensional (3D) biomimetic cell culture platforms offer more realistic microenvironments that cells naturally experience in vivo.
32056374	8	15	theme	biochemical	1499:1509	arg1	composition					1511:1521	their biochemical composition	1493:1521	their biochemical composition	1493:1521	The as-desired HA-based 3D tumor-like models we developed may provide a useful platform for the study of various cancer cells by simply tuning their biochemical composition and their mechanical properties.
32056374	6	16	theme	stiffness-dependent	1201:1219	arg1	manner					1221:1226	a stiffness-dependent manner	1199:1226	a stiffness-dependent manner	1199:1226	While PLL-treated hydrogels were not favorable to the culture of any glioblastoma cell lines, they enhanced the proliferation of breast cancer cells in a stiffness-dependent manner.
32056374	5	17	theme	enzyme	954:959	arg1	resistance					961:970	(b) efficient enzyme resistance	940:970	(b) efficient enzyme resistance	940:970	Overall, our hydrogels exhibited (a) increasing stiffness with GnP concentration for every pre-functionalization and (b) efficient enzyme resistance with PLL treatment, and also with type IV collagen but to a lesser extent.
32056374	8	18	theme	HA-based	1365:1372	arg1	models					1388:1393	The as-desired HA-based 3D tumor-like models	1350:1393	The as-desired HA-based 3D tumor-like models we developed	1350:1406	The as-desired HA-based 3D tumor-like models we developed may provide a useful platform for the study of various cancer cells by simply tuning their biochemical composition and their mechanical properties.
32056374	5	19	theme	PLL	977:979	arg1	treatment					981:989	PLL treatment	977:989	PLL treatment	977:989	Overall, our hydrogels exhibited (a) increasing stiffness with GnP concentration for every pre-functionalization and (b) efficient enzyme resistance with PLL treatment, and also with type IV collagen but to a lesser extent.
32056374	3	20	theme	tumorous	557:564	arg1	tissues					566:572	tumorous tissues	557:572	tumorous tissues	557:572	For that, we pre-functionalized our hydrogels with an adhesive polypeptide (poly-l-lysine, PLL) or ECM proteins (type III and type IV collagens), naturally present in tumorous tissues, and next, we tuned their stiffness by crosslinking with gradual concentrations of genipin (GnP).
32056374	3	21	theme	adhesive	444:451	arg1	polypeptide					453:463	an adhesive polypeptide	441:463	an adhesive polypeptide (poly-l-lysine, PLL)	441:484	For that, we pre-functionalized our hydrogels with an adhesive polypeptide (poly-l-lysine, PLL) or ECM proteins (type III and type IV collagens), naturally present in tumorous tissues, and next, we tuned their stiffness by crosslinking with gradual concentrations of genipin (GnP).
32056374	0	22	theme	Hyaluronan-based	0:15	arg1	stiffness					75:83	stiffness	75:83	stiffness with genipin-crosslinking	75:109	Hyaluronan-based hydrogels as versatile tumor-like models: Tunable ECM and stiffness with genipin-crosslinking.
32056374	0	22	theme	Hyaluronan-based	0:15	arg1	ECM					67:69	Tunable ECM	59:69	Tunable ECM	59:69	Hyaluronan-based hydrogels as versatile tumor-like models: Tunable ECM and stiffness with genipin-crosslinking.
32056374	0	22	theme	Hyaluronan-based	0:15	arg1	hydrogels					17:25	Hyaluronan-based hydrogels	0:25	Hyaluronan-based hydrogels as versatile tumor-like models: Tunable ECM and stiffness with genipin-crosslinking.	0:110	Hyaluronan-based hydrogels as versatile tumor-like models: Tunable ECM and stiffness with genipin-crosslinking.
32056374	2	23	theme	healthy	338:344	arg1	matrices					373:380	healthy or malignant extracellular matrices	338:380	healthy or malignant extracellular matrices (ECMs)	338:387	We developed a tunable hyaluronan-based hydrogels that could easily be modified to mimic healthy or malignant extracellular matrices (ECMs).
32056374	2	23	theme	healthy	338:344	arg1	ECMs					383:386	ECMs	383:386	ECMs	383:386	We developed a tunable hyaluronan-based hydrogels that could easily be modified to mimic healthy or malignant extracellular matrices (ECMs).
32056374	7	24	theme	collagen	1268:1275	arg1	hydrogels					1289:1297	type IV collagen pre-treated hydrogels	1260:1297	type IV collagen pre-treated hydrogels	1260:1297	Contrary to type III collagen, type IV collagen pre-treated hydrogels supported the proliferation of glioblastoma cells.
32056374	0	25	theme	versatile	30:38	arg1	models					51:56	versatile tumor-like models	30:56	versatile tumor-like models	30:56	Hyaluronan-based hydrogels as versatile tumor-like models: Tunable ECM and stiffness with genipin-crosslinking.
32056374	0	26	dep	hydrogels	17:25	arg1	stiffness					75:83	stiffness	75:83	stiffness with genipin-crosslinking	75:109	Hyaluronan-based hydrogels as versatile tumor-like models: Tunable ECM and stiffness with genipin-crosslinking.
32056374	0	26	dep	hydrogels	17:25	arg1	ECM					67:69	Tunable ECM	59:69	Tunable ECM	59:69	Hyaluronan-based hydrogels as versatile tumor-like models: Tunable ECM and stiffness with genipin-crosslinking.
32056374	0	26	dep	hydrogels	17:25	arg1	hydrogels					17:25	Hyaluronan-based hydrogels	0:25	Hyaluronan-based hydrogels as versatile tumor-like models: Tunable ECM and stiffness with genipin-crosslinking.	0:110	Hyaluronan-based hydrogels as versatile tumor-like models: Tunable ECM and stiffness with genipin-crosslinking.
32056374	8	27	theme	3D	1374:1375	arg1	models					1388:1393	The as-desired HA-based 3D tumor-like models	1350:1393	The as-desired HA-based 3D tumor-like models we developed	1350:1406	The as-desired HA-based 3D tumor-like models we developed may provide a useful platform for the study of various cancer cells by simply tuning their biochemical composition and their mechanical properties.
32056374	1	28	theme	Three-dimensional	112:128	arg1	platforms					159:167	Three-dimensional (3D) biomimetic cell culture platforms	112:167	Three-dimensional (3D) biomimetic cell culture platforms	112:167	Three-dimensional (3D) biomimetic cell culture platforms offer more realistic microenvironments that cells naturally experience in vivo.
32056374	3	29	with	crosslinking	613:624	arg1	concentrations					639:652	gradual concentrations	631:652	gradual concentrations of genipin (GnP)	631:669	For that, we pre-functionalized our hydrogels with an adhesive polypeptide (poly-l-lysine, PLL) or ECM proteins (type III and type IV collagens), naturally present in tumorous tissues, and next, we tuned their stiffness by crosslinking with gradual concentrations of genipin (GnP).
32056374	8	30	theme	mechanical	1533:1542	arg1	properties					1544:1553	their mechanical properties	1527:1553	their mechanical properties	1527:1553	The as-desired HA-based 3D tumor-like models we developed may provide a useful platform for the study of various cancer cells by simply tuning their biochemical composition and their mechanical properties.
32056374	6	31	theme	cell	1129:1132	arg1	lines					1134:1138	any glioblastoma cell lines	1112:1138	any glioblastoma cell lines	1112:1138	While PLL-treated hydrogels were not favorable to the culture of any glioblastoma cell lines, they enhanced the proliferation of breast cancer cells in a stiffness-dependent manner.
32056374	8	32	theme	as-desired	1354:1363	arg1	models					1388:1393	The as-desired HA-based 3D tumor-like models	1350:1393	The as-desired HA-based 3D tumor-like models we developed	1350:1406	The as-desired HA-based 3D tumor-like models we developed may provide a useful platform for the study of various cancer cells by simply tuning their biochemical composition and their mechanical properties.
32056374	8	33	dep	models	1388:1393	arg1	we					1395:1396	we	1395:1396	we	1395:1396	The as-desired HA-based 3D tumor-like models we developed may provide a useful platform for the study of various cancer cells by simply tuning their biochemical composition and their mechanical properties.
32056374	3	34	theme	type	516:519	arg1	collagens					524:532	type III and type IV collagens	503:532	collagens	524:532	For that, we pre-functionalized our hydrogels with an adhesive polypeptide (poly-l-lysine, PLL) or ECM proteins (type III and type IV collagens), naturally present in tumorous tissues, and next, we tuned their stiffness by crosslinking with gradual concentrations of genipin (GnP).
32056374	7	35	theme	type	1241:1244	arg1	collagen					1250:1257	type III collagen	1241:1257	type III collagen	1241:1257	Contrary to type III collagen, type IV collagen pre-treated hydrogels supported the proliferation of glioblastoma cells.
32056374	6	36	theme	breast	1176:1181	arg1	cells					1190:1194	breast cancer cells	1176:1194	breast cancer cells	1176:1194	While PLL-treated hydrogels were not favorable to the culture of any glioblastoma cell lines, they enhanced the proliferation of breast cancer cells in a stiffness-dependent manner.
32056374	3	37	theme	gradual	631:637	arg1	concentrations					639:652	gradual concentrations	631:652	gradual concentrations of genipin (GnP)	631:669	For that, we pre-functionalized our hydrogels with an adhesive polypeptide (poly-l-lysine, PLL) or ECM proteins (type III and type IV collagens), naturally present in tumorous tissues, and next, we tuned their stiffness by crosslinking with gradual concentrations of genipin (GnP).
32056374	5	38	theme	type	1006:1009	arg1	collagen					1014:1021	type IV collagen	1006:1021	type IV collagen	1006:1021	Overall, our hydrogels exhibited (a) increasing stiffness with GnP concentration for every pre-functionalization and (b) efficient enzyme resistance with PLL treatment, and also with type IV collagen but to a lesser extent.
32056374	5	39	dep	resistance	961:970	arg1	b					941:941	b	941:941	b	941:941	Overall, our hydrogels exhibited (a) increasing stiffness with GnP concentration for every pre-functionalization and (b) efficient enzyme resistance with PLL treatment, and also with type IV collagen but to a lesser extent.
32056374	0	40	theme	tumor-like	40:49	arg1	models					51:56	versatile tumor-like models	30:56	versatile tumor-like models	30:56	Hyaluronan-based hydrogels as versatile tumor-like models: Tunable ECM and stiffness with genipin-crosslinking.
32056374	5	41	theme	lesser	1032:1037	arg1	extent					1039:1044	a lesser extent	1030:1044	a lesser extent	1030:1044	Overall, our hydrogels exhibited (a) increasing stiffness with GnP concentration for every pre-functionalization and (b) efficient enzyme resistance with PLL treatment, and also with type IV collagen but to a lesser extent.
32056374	3	42	dep	polypeptide	453:463	arg1	PLL					481:483	PLL	481:483	PLL	481:483	For that, we pre-functionalized our hydrogels with an adhesive polypeptide (poly-l-lysine, PLL) or ECM proteins (type III and type IV collagens), naturally present in tumorous tissues, and next, we tuned their stiffness by crosslinking with gradual concentrations of genipin (GnP).
32056374	3	42	dep	polypeptide	453:463	arg1	poly-l-lysine					466:478	poly-l-lysine	466:478	poly-l-lysine	466:478	For that, we pre-functionalized our hydrogels with an adhesive polypeptide (poly-l-lysine, PLL) or ECM proteins (type III and type IV collagens), naturally present in tumorous tissues, and next, we tuned their stiffness by crosslinking with gradual concentrations of genipin (GnP).
32056374	0	43	theme	Tunable	59:65	arg1	ECM					67:69	Tunable ECM	59:69	Tunable ECM	59:69	Hyaluronan-based hydrogels as versatile tumor-like models: Tunable ECM and stiffness with genipin-crosslinking.
32056374	0	43	theme	Tunable	59:65	arg1	hydrogels					17:25	Hyaluronan-based hydrogels	0:25	Hyaluronan-based hydrogels as versatile tumor-like models: Tunable ECM and stiffness with genipin-crosslinking.	0:110	Hyaluronan-based hydrogels as versatile tumor-like models: Tunable ECM and stiffness with genipin-crosslinking.
32056374	8	44	theme	useful	1422:1427	arg1	platform					1429:1436	a useful platform	1420:1436	a useful platform for the study of various cancer cells	1420:1474	The as-desired HA-based 3D tumor-like models we developed may provide a useful platform for the study of various cancer cells by simply tuning their biochemical composition and their mechanical properties.
32056374	4	45	theme	endothelial	804:814	arg1	cells					816:820	endothelial cells	804:820	endothelial cells	804:820	Then, we thoroughly characterized our substrates before testing them with glioblastoma and breast cancer cells, and thereafter with endothelial cells.
32056374	8	46	theme	cells	1470:1474	arg1	study					1446:1450	the study	1442:1450	the study of various cancer cells	1442:1474	The as-desired HA-based 3D tumor-like models we developed may provide a useful platform for the study of various cancer cells by simply tuning their biochemical composition and their mechanical properties.
32056374	8	47	theme	tumor-like	1377:1386	arg1	models					1388:1393	The as-desired HA-based 3D tumor-like models	1350:1393	The as-desired HA-based 3D tumor-like models we developed	1350:1406	The as-desired HA-based 3D tumor-like models we developed may provide a useful platform for the study of various cancer cells by simply tuning their biochemical composition and their mechanical properties.
32056374	3	48	theme	ECM	489:491	arg1	proteins					493:500	ECM proteins	489:500	ECM proteins (type III and type IV collagens)	489:533	For that, we pre-functionalized our hydrogels with an adhesive polypeptide (poly-l-lysine, PLL) or ECM proteins (type III and type IV collagens), naturally present in tumorous tissues, and next, we tuned their stiffness by crosslinking with gradual concentrations of genipin (GnP).
32056374	3	49	theme	genipin	657:663	arg1	concentrations					639:652	gradual concentrations	631:652	gradual concentrations of genipin (GnP)	631:669	For that, we pre-functionalized our hydrogels with an adhesive polypeptide (poly-l-lysine, PLL) or ECM proteins (type III and type IV collagens), naturally present in tumorous tissues, and next, we tuned their stiffness by crosslinking with gradual concentrations of genipin (GnP).
32056374	7	50	theme	cells	1343:1347	arg1	proliferation					1313:1325	the proliferation	1309:1325	the proliferation of glioblastoma cells	1309:1347	Contrary to type III collagen, type IV collagen pre-treated hydrogels supported the proliferation of glioblastoma cells.
32056374	5	51	theme	GnP	886:888	arg1	concentration					890:902	GnP concentration	886:902	GnP concentration	886:902	Overall, our hydrogels exhibited (a) increasing stiffness with GnP concentration for every pre-functionalization and (b) efficient enzyme resistance with PLL treatment, and also with type IV collagen but to a lesser extent.
32056374	3	52	from	present	546:552	arg1	tissues					566:572	tumorous tissues	557:572	tumorous tissues	557:572	For that, we pre-functionalized our hydrogels with an adhesive polypeptide (poly-l-lysine, PLL) or ECM proteins (type III and type IV collagens), naturally present in tumorous tissues, and next, we tuned their stiffness by crosslinking with gradual concentrations of genipin (GnP).
32056374	1	53	theme	culture	151:157	arg1	platforms					159:167	Three-dimensional (3D) biomimetic cell culture platforms	112:167	Three-dimensional (3D) biomimetic cell culture platforms	112:167	Three-dimensional (3D) biomimetic cell culture platforms offer more realistic microenvironments that cells naturally experience in vivo.
32056374	4	54	theme	cancer	770:775	arg1	cells					777:781	breast cancer cells	763:781	breast cancer cells	763:781	Then, we thoroughly characterized our substrates before testing them with glioblastoma and breast cancer cells, and thereafter with endothelial cells.
32056374	6	55	theme	cancer	1183:1188	arg1	cells					1190:1194	breast cancer cells	1176:1194	breast cancer cells	1176:1194	While PLL-treated hydrogels were not favorable to the culture of any glioblastoma cell lines, they enhanced the proliferation of breast cancer cells in a stiffness-dependent manner.
32056374	1	56	theme	biomimetic	135:144	arg1	platforms					159:167	Three-dimensional (3D) biomimetic cell culture platforms	112:167	Three-dimensional (3D) biomimetic cell culture platforms	112:167	Three-dimensional (3D) biomimetic cell culture platforms offer more realistic microenvironments that cells naturally experience in vivo.
32056374	6	57	theme	glioblastoma	1116:1127	arg1	lines					1134:1138	any glioblastoma cell lines	1112:1138	any glioblastoma cell lines	1112:1138	While PLL-treated hydrogels were not favorable to the culture of any glioblastoma cell lines, they enhanced the proliferation of breast cancer cells in a stiffness-dependent manner.
32056374	4	58	theme	breast	763:768	arg1	cells					777:781	breast cancer cells	763:781	breast cancer cells	763:781	Then, we thoroughly characterized our substrates before testing them with glioblastoma and breast cancer cells, and thereafter with endothelial cells.
32056374	2	59	theme	malignant	349:357	arg1	matrices					373:380	healthy or malignant extracellular matrices	338:380	healthy or malignant extracellular matrices (ECMs)	338:387	We developed a tunable hyaluronan-based hydrogels that could easily be modified to mimic healthy or malignant extracellular matrices (ECMs).
32056374	2	59	theme	malignant	349:357	arg1	ECMs					383:386	ECMs	383:386	ECMs	383:386	We developed a tunable hyaluronan-based hydrogels that could easily be modified to mimic healthy or malignant extracellular matrices (ECMs).
32056374	2	60	theme	extracellular	359:371	arg1	matrices					373:380	healthy or malignant extracellular matrices	338:380	healthy or malignant extracellular matrices (ECMs)	338:387	We developed a tunable hyaluronan-based hydrogels that could easily be modified to mimic healthy or malignant extracellular matrices (ECMs).
32056374	2	60	theme	extracellular	359:371	arg1	ECMs					383:386	ECMs	383:386	ECMs	383:386	We developed a tunable hyaluronan-based hydrogels that could easily be modified to mimic healthy or malignant extracellular matrices (ECMs).
32056374	2	61	theme	hyaluronan-based	272:287	arg1	hydrogels					289:297	a tunable hyaluronan-based hydrogels	262:297	a tunable hyaluronan-based hydrogels that could easily be modified to mimic healthy or malignant extracellular matrices (ECMs)	262:387	We developed a tunable hyaluronan-based hydrogels that could easily be modified to mimic healthy or malignant extracellular matrices (ECMs).
32056374	1	62	dep	Three-dimensional	112:128	arg1	3D					131:132	3D	131:132	3D	131:132	Three-dimensional (3D) biomimetic cell culture platforms offer more realistic microenvironments that cells naturally experience in vivo.
32056374	3	63	from	tissues	566:572	arg1	present					546:552	present	546:552	present	546:552	For that, we pre-functionalized our hydrogels with an adhesive polypeptide (poly-l-lysine, PLL) or ECM proteins (type III and type IV collagens), naturally present in tumorous tissues, and next, we tuned their stiffness by crosslinking with gradual concentrations of genipin (GnP).
32056374	8	64	theme	cancer	1463:1468	arg1	cells					1470:1474	various cancer cells	1455:1474	various cancer cells	1455:1474	The as-desired HA-based 3D tumor-like models we developed may provide a useful platform for the study of various cancer cells by simply tuning their biochemical composition and their mechanical properties.
32056374	2	65	theme	tunable	264:270	arg1	hydrogels					289:297	a tunable hyaluronan-based hydrogels	262:297	a tunable hyaluronan-based hydrogels that could easily be modified to mimic healthy or malignant extracellular matrices (ECMs)	262:387	We developed a tunable hyaluronan-based hydrogels that could easily be modified to mimic healthy or malignant extracellular matrices (ECMs).
32056374	6	66	theme	PLL-treated	1053:1063	arg1	hydrogels					1065:1073	PLL-treated hydrogels	1053:1073	PLL-treated hydrogels	1053:1073	While PLL-treated hydrogels were not favorable to the culture of any glioblastoma cell lines, they enhanced the proliferation of breast cancer cells in a stiffness-dependent manner.
34757130	8	0	theme	β-glucan	1211:1218	arg1	brooders					1224:1231	0.5 and 1% β-glucan fed brooders	1200:1231	0.5 and 1% β-glucan fed brooders	1200:1231	On the other hand, the higher seminal plasma total antioxidant capacity observed in 0.5 and 1% β-glucan fed brooders indicated increased scavenging ability of reactive oxygen species.
34757130	0	1	dep	putitora	156:163	arg1	Hamilton					166:173	Hamilton	166:173	Hamilton	166:173	Dietary β-glucan influences the expression of testicular aquaporins, antioxidative defence genes and sperm quality traits in endangered golden mahseer, Tor putitora (Hamilton, 1822).
34757130	1	2	theme	sperm	245:249	arg1	characteristics					251:265	sperm characteristics	245:265	sperm characteristics	245:265	The effect of dietary β-glucan on seminal plasma composition, sperm characteristics, expression of aquaporins, and antioxidative defence genes of golden mahseer was evaluated.
34757130	5	3	theme	1.5	782:784	arg1	%					785:785	%	785:785	%	785:785	The marked down-regulation in the transcript abundance of testicular aqp3a noticed in 1.5% β-glucan fed brooders corresponds to their poor sperm quality.
34757130	7	4	dep	control	994:1000	arg1	groups					1035:1040	groups	1035:1040	groups	1035:1040	In contrast, control and higher β-glucan (1 and 1.5%) groups displayed relatively higher expression levels of testicular gst and sod1.
34757130	5	5	theme	β-glucan	787:794	arg1	brooders					800:807	1.5% β-glucan fed brooders	782:807	1.5% β-glucan fed brooders	782:807	The marked down-regulation in the transcript abundance of testicular aqp3a noticed in 1.5% β-glucan fed brooders corresponds to their poor sperm quality.
34757130	8	6	theme	other	1123:1127	arg1	hand					1129:1132	the other hand	1119:1132	the other hand	1119:1132	On the other hand, the higher seminal plasma total antioxidant capacity observed in 0.5 and 1% β-glucan fed brooders indicated increased scavenging ability of reactive oxygen species.
34757130	9	7	theme	0.5	1328:1330	arg1	%					1331:1331	%	1331:1331	%	1331:1331	Overall, supplementation of 0.5% β-glucan improved sperm quality and antioxidative potential, but the higher inclusion (1.5%) negatively affected sperm characteristics.
34757130	6	8	theme	mRNA	863:866	arg1	lowest					943:948	lowest	943:948	lowest	943:948	Further, the mRNA expression of genes encoding antioxidant enzymes, namely gst and sod1, was lowest in 0.5% β-glucan fed brooders.
34757130	6	8	theme	mRNA	863:866	arg1	expression					868:877	the mRNA expression	859:877	the mRNA expression of genes encoding antioxidant enzymes, namely gst and sod1,	859:937	Further, the mRNA expression of genes encoding antioxidant enzymes, namely gst and sod1, was lowest in 0.5% β-glucan fed brooders.
34757130	2	9	theme	mahseer	467:473	arg1	brooders					475:482	male golden mahseer brooders	455:482	male golden mahseer brooders	455:482	For that, four experimental diets containing 0 (control), 0.5, 1, and 1.5% β-glucan were fed to male golden mahseer brooders for 130 days.
34757130	10	10	theme	dietary	1483:1489	arg1	%					1504:1504	0.5%	1501:1504	0.5%	1501:1504	Collectively, dietary β-glucan (0.5%) can be a practical approach to developing quality broodstock of golden mahseer.
34757130	10	10	theme	dietary	1483:1489	arg1	approach					1526:1533	a practical approach	1514:1533	a practical approach to developing quality broodstock of golden mahseer	1514:1584	Collectively, dietary β-glucan (0.5%) can be a practical approach to developing quality broodstock of golden mahseer.
34757130	10	10	theme	dietary	1483:1489	arg1	β-glucan					1491:1498	dietary β-glucan	1483:1498	dietary β-glucan (0.5%)	1483:1505	Collectively, dietary β-glucan (0.5%) can be a practical approach to developing quality broodstock of golden mahseer.
34757130	0	11	theme	golden	136:141	arg1	mahseer					143:149	endangered golden mahseer	125:149	endangered golden mahseer	125:149	Dietary β-glucan influences the expression of testicular aquaporins, antioxidative defence genes and sperm quality traits in endangered golden mahseer, Tor putitora (Hamilton, 1822).
34757130	1	12	from	effect	187:192	arg1	expression					268:277	expression	268:277	expression of aquaporins	268:291	The effect of dietary β-glucan on seminal plasma composition, sperm characteristics, expression of aquaporins, and antioxidative defence genes of golden mahseer was evaluated.
34757130	1	12	from	effect	187:192	arg1	composition					232:242	seminal plasma composition	217:242	seminal plasma composition	217:242	The effect of dietary β-glucan on seminal plasma composition, sperm characteristics, expression of aquaporins, and antioxidative defence genes of golden mahseer was evaluated.
34757130	1	12	from	effect	187:192	arg1	characteristics					251:265	sperm characteristics	245:265	sperm characteristics	245:265	The effect of dietary β-glucan on seminal plasma composition, sperm characteristics, expression of aquaporins, and antioxidative defence genes of golden mahseer was evaluated.
34757130	1	12	from	effect	187:192	arg1	genes					320:324	antioxidative defence genes	298:324	antioxidative defence genes of golden mahseer	298:342	The effect of dietary β-glucan on seminal plasma composition, sperm characteristics, expression of aquaporins, and antioxidative defence genes of golden mahseer was evaluated.
34757130	9	13	theme	β-glucan	1333:1340	arg1	supplementation					1309:1323	supplementation	1309:1323	supplementation of 0.5% β-glucan	1309:1340	Overall, supplementation of 0.5% β-glucan improved sperm quality and antioxidative potential, but the higher inclusion (1.5%) negatively affected sperm characteristics.
34757130	8	14	theme	higher	1139:1144	arg1	capacity					1179:1186	the higher seminal plasma total antioxidant capacity	1135:1186	the higher seminal plasma total antioxidant capacity observed in 0.5 and 1% β-glucan fed brooders	1135:1231	On the other hand, the higher seminal plasma total antioxidant capacity observed in 0.5 and 1% β-glucan fed brooders indicated increased scavenging ability of reactive oxygen species.
34757130	3	15	theme	%	512:512	arg1	β-glucan					514:521	0.5% β-glucan	509:521	0.5% β-glucan	509:521	Feeding of 0.5% β-glucan was found to improve sperm characteristics, viz.
34757130	5	16	theme	poor	830:833	arg1	quality					841:847	their poor sperm quality	824:847	their poor sperm quality	824:847	The marked down-regulation in the transcript abundance of testicular aqp3a noticed in 1.5% β-glucan fed brooders corresponds to their poor sperm quality.
34757130	8	17	theme	plasma	1154:1159	arg1	capacity					1179:1186	the higher seminal plasma total antioxidant capacity	1135:1186	the higher seminal plasma total antioxidant capacity observed in 0.5 and 1% β-glucan fed brooders	1135:1231	On the other hand, the higher seminal plasma total antioxidant capacity observed in 0.5 and 1% β-glucan fed brooders indicated increased scavenging ability of reactive oxygen species.
34757130	2	18	contain	containing	393:402	arg2	0					404:404	0	404:404	0	404:404	For that, four experimental diets containing 0 (control), 0.5, 1, and 1.5% β-glucan were fed to male golden mahseer brooders for 130 days.
34757130	2	18	contain	containing	393:402	arg2	0.5					417:419	0.5	417:419	0.5	417:419	For that, four experimental diets containing 0 (control), 0.5, 1, and 1.5% β-glucan were fed to male golden mahseer brooders for 130 days.
34757130	2	18	contain	containing	393:402	arg2	%					432:432	1.5% β-glucan	429:441	1.5% β-glucan	429:441	For that, four experimental diets containing 0 (control), 0.5, 1, and 1.5% β-glucan were fed to male golden mahseer brooders for 130 days.
34757130	2	18	contain	containing	393:402	arg2	1					422:422	1	422:422	1	422:422	For that, four experimental diets containing 0 (control), 0.5, 1, and 1.5% β-glucan were fed to male golden mahseer brooders for 130 days.
34757130	2	18	contain	containing	393:402	arg1	diets					387:391	four experimental diets	369:391	four experimental diets containing 0 (control), 0.5, 1, and 1.5% β-glucan	369:441	For that, four experimental diets containing 0 (control), 0.5, 1, and 1.5% β-glucan were fed to male golden mahseer brooders for 130 days.
34757130	2	18	contain	containing	393:402	arg2	control					407:413	control	407:413	control	407:413	For that, four experimental diets containing 0 (control), 0.5, 1, and 1.5% β-glucan were fed to male golden mahseer brooders for 130 days.
34757130	3	19	theme	sperm	544:548	arg1	viz					567:569	viz	567:569	viz	567:569	Feeding of 0.5% β-glucan was found to improve sperm characteristics, viz.
34757130	3	19	theme	sperm	544:548	arg1	characteristics					550:564	sperm characteristics	544:564	sperm characteristics	544:564	Feeding of 0.5% β-glucan was found to improve sperm characteristics, viz.
34757130	2	20	theme	β-glucan	434:441	arg1	%					432:432	1.5% β-glucan	429:441	1.5% β-glucan	429:441	For that, four experimental diets containing 0 (control), 0.5, 1, and 1.5% β-glucan were fed to male golden mahseer brooders for 130 days.
34757130	8	21	theme	antioxidant	1167:1177	arg1	capacity					1179:1186	the higher seminal plasma total antioxidant capacity	1135:1186	the higher seminal plasma total antioxidant capacity observed in 0.5 and 1% β-glucan fed brooders	1135:1231	On the other hand, the higher seminal plasma total antioxidant capacity observed in 0.5 and 1% β-glucan fed brooders indicated increased scavenging ability of reactive oxygen species.
34757130	7	22	theme	gst	1102:1104	arg1	levels					1081:1086	relatively higher expression levels	1052:1086	relatively higher expression levels of testicular gst and sod1	1052:1113	In contrast, control and higher β-glucan (1 and 1.5%) groups displayed relatively higher expression levels of testicular gst and sod1.
34757130	1	23	theme	defence	312:318	arg1	genes					320:324	antioxidative defence genes	298:324	antioxidative defence genes of golden mahseer	298:342	The effect of dietary β-glucan on seminal plasma composition, sperm characteristics, expression of aquaporins, and antioxidative defence genes of golden mahseer was evaluated.
34757130	0	24	theme	defence	83:89	arg1	genes					91:95	antioxidative defence genes	69:95	antioxidative defence genes	69:95	Dietary β-glucan influences the expression of testicular aquaporins, antioxidative defence genes and sperm quality traits in endangered golden mahseer, Tor putitora (Hamilton, 1822).
34757130	0	24	theme	defence	83:89	arg1	aquaporins					57:66	testicular aquaporins	46:66	testicular aquaporins	46:66	Dietary β-glucan influences the expression of testicular aquaporins, antioxidative defence genes and sperm quality traits in endangered golden mahseer, Tor putitora (Hamilton, 1822).
34757130	2	25	theme	male	455:458	arg1	brooders					475:482	male golden mahseer brooders	455:482	male golden mahseer brooders	455:482	For that, four experimental diets containing 0 (control), 0.5, 1, and 1.5% β-glucan were fed to male golden mahseer brooders for 130 days.
34757130	4	26	theme	plasma	671:676	arg1	resources					685:693	seminal plasma energy resources	663:693	seminal plasma energy resources	663:693	sperm count, motility, viability, and morphology with no effect on gonadosomatic index and seminal plasma energy resources.
34757130	7	27	theme	expression	1070:1079	arg1	levels					1081:1086	relatively higher expression levels	1052:1086	relatively higher expression levels of testicular gst and sod1	1052:1113	In contrast, control and higher β-glucan (1 and 1.5%) groups displayed relatively higher expression levels of testicular gst and sod1.
34757130	1	28	theme	mahseer	336:342	arg1	expression					268:277	expression	268:277	expression of aquaporins	268:291	The effect of dietary β-glucan on seminal plasma composition, sperm characteristics, expression of aquaporins, and antioxidative defence genes of golden mahseer was evaluated.
34757130	1	28	theme	mahseer	336:342	arg1	composition					232:242	seminal plasma composition	217:242	seminal plasma composition	217:242	The effect of dietary β-glucan on seminal plasma composition, sperm characteristics, expression of aquaporins, and antioxidative defence genes of golden mahseer was evaluated.
34757130	1	28	theme	mahseer	336:342	arg1	characteristics					251:265	sperm characteristics	245:265	sperm characteristics	245:265	The effect of dietary β-glucan on seminal plasma composition, sperm characteristics, expression of aquaporins, and antioxidative defence genes of golden mahseer was evaluated.
34757130	1	28	theme	mahseer	336:342	arg1	genes					320:324	antioxidative defence genes	298:324	antioxidative defence genes of golden mahseer	298:342	The effect of dietary β-glucan on seminal plasma composition, sperm characteristics, expression of aquaporins, and antioxidative defence genes of golden mahseer was evaluated.
34757130	9	29	theme	higher	1402:1407	arg1	inclusion					1409:1417	the higher inclusion	1398:1417	the higher inclusion (1.5%)	1398:1424	Overall, supplementation of 0.5% β-glucan improved sperm quality and antioxidative potential, but the higher inclusion (1.5%) negatively affected sperm characteristics.
34757130	9	29	theme	higher	1402:1407	arg1	%					1423:1423	1.5%	1420:1423	1.5%	1420:1423	Overall, supplementation of 0.5% β-glucan improved sperm quality and antioxidative potential, but the higher inclusion (1.5%) negatively affected sperm characteristics.
34757130	0	30	theme	quality	107:113	arg1	aquaporins					57:66	testicular aquaporins	46:66	testicular aquaporins	46:66	Dietary β-glucan influences the expression of testicular aquaporins, antioxidative defence genes and sperm quality traits in endangered golden mahseer, Tor putitora (Hamilton, 1822).
34757130	0	30	theme	quality	107:113	arg1	traits					115:120	sperm quality traits	101:120	sperm quality traits	101:120	Dietary β-glucan influences the expression of testicular aquaporins, antioxidative defence genes and sperm quality traits in endangered golden mahseer, Tor putitora (Hamilton, 1822).
34757130	6	31	theme	β-glucan	958:965	arg1	brooders					971:978	0.5% β-glucan fed brooders	953:978	0.5% β-glucan fed brooders	953:978	Further, the mRNA expression of genes encoding antioxidant enzymes, namely gst and sod1, was lowest in 0.5% β-glucan fed brooders.
34757130	0	32	theme	antioxidative	69:81	arg1	genes					91:95	antioxidative defence genes	69:95	antioxidative defence genes	69:95	Dietary β-glucan influences the expression of testicular aquaporins, antioxidative defence genes and sperm quality traits in endangered golden mahseer, Tor putitora (Hamilton, 1822).
34757130	0	32	theme	antioxidative	69:81	arg1	aquaporins					57:66	testicular aquaporins	46:66	testicular aquaporins	46:66	Dietary β-glucan influences the expression of testicular aquaporins, antioxidative defence genes and sperm quality traits in endangered golden mahseer, Tor putitora (Hamilton, 1822).
34757130	4	33	theme	gonadosomatic	639:651	arg1	index					653:657	gonadosomatic index	639:657	gonadosomatic index	639:657	sperm count, motility, viability, and morphology with no effect on gonadosomatic index and seminal plasma energy resources.
34757130	0	34	theme	Dietary	0:6	arg1	β-glucan					8:15	Dietary β-glucan	0:15	Dietary β-glucan	0:15	Dietary β-glucan influences the expression of testicular aquaporins, antioxidative defence genes and sperm quality traits in endangered golden mahseer, Tor putitora (Hamilton, 1822).
34757130	6	35	theme	0.5	953:955	arg1	%					956:956	%	956:956	%	956:956	Further, the mRNA expression of genes encoding antioxidant enzymes, namely gst and sod1, was lowest in 0.5% β-glucan fed brooders.
34757130	10	36	theme	practical	1516:1524	arg1	approach					1526:1533	a practical approach	1514:1533	a practical approach to developing quality broodstock of golden mahseer	1514:1584	Collectively, dietary β-glucan (0.5%) can be a practical approach to developing quality broodstock of golden mahseer.
34757130	10	36	theme	practical	1516:1524	arg1	β-glucan					1491:1498	dietary β-glucan	1483:1498	dietary β-glucan (0.5%)	1483:1505	Collectively, dietary β-glucan (0.5%) can be a practical approach to developing quality broodstock of golden mahseer.
34757130	5	37	theme	fed	796:798	arg1	brooders					800:807	1.5% β-glucan fed brooders	782:807	1.5% β-glucan fed brooders	782:807	The marked down-regulation in the transcript abundance of testicular aqp3a noticed in 1.5% β-glucan fed brooders corresponds to their poor sperm quality.
34757130	8	38	theme	increased	1243:1251	arg1	ability					1264:1270	increased scavenging ability	1243:1270	increased scavenging ability of reactive oxygen species	1243:1297	On the other hand, the higher seminal plasma total antioxidant capacity observed in 0.5 and 1% β-glucan fed brooders indicated increased scavenging ability of reactive oxygen species.
34757130	1	39	theme	aquaporins	282:291	arg1	expression					268:277	expression	268:277	expression of aquaporins	268:291	The effect of dietary β-glucan on seminal plasma composition, sperm characteristics, expression of aquaporins, and antioxidative defence genes of golden mahseer was evaluated.
34757130	1	39	theme	aquaporins	282:291	arg1	composition					232:242	seminal plasma composition	217:242	seminal plasma composition	217:242	The effect of dietary β-glucan on seminal plasma composition, sperm characteristics, expression of aquaporins, and antioxidative defence genes of golden mahseer was evaluated.
34757130	1	39	theme	aquaporins	282:291	arg1	characteristics					251:265	sperm characteristics	245:265	sperm characteristics	245:265	The effect of dietary β-glucan on seminal plasma composition, sperm characteristics, expression of aquaporins, and antioxidative defence genes of golden mahseer was evaluated.
34757130	1	39	theme	aquaporins	282:291	arg1	genes					320:324	antioxidative defence genes	298:324	antioxidative defence genes of golden mahseer	298:342	The effect of dietary β-glucan on seminal plasma composition, sperm characteristics, expression of aquaporins, and antioxidative defence genes of golden mahseer was evaluated.
34757130	9	40	theme	sperm	1446:1450	arg1	characteristics					1452:1466	sperm characteristics	1446:1466	sperm characteristics	1446:1466	Overall, supplementation of 0.5% β-glucan improved sperm quality and antioxidative potential, but the higher inclusion (1.5%) negatively affected sperm characteristics.
34757130	4	41	with	sperm	572:576	arg1	effect					629:634	no effect	626:634	no effect on gonadosomatic index and seminal plasma energy resources	626:693	sperm count, motility, viability, and morphology with no effect on gonadosomatic index and seminal plasma energy resources.
34757130	10	42	theme	quality	1549:1555	arg1	broodstock					1557:1566	quality broodstock	1549:1566	quality broodstock of golden mahseer	1549:1584	Collectively, dietary β-glucan (0.5%) can be a practical approach to developing quality broodstock of golden mahseer.
34757130	0	43	theme	testicular	46:55	arg1	traits					115:120	sperm quality traits	101:120	sperm quality traits	101:120	Dietary β-glucan influences the expression of testicular aquaporins, antioxidative defence genes and sperm quality traits in endangered golden mahseer, Tor putitora (Hamilton, 1822).
34757130	0	43	theme	testicular	46:55	arg1	putitora					156:163	putitora	156:163	putitora	156:163	Dietary β-glucan influences the expression of testicular aquaporins, antioxidative defence genes and sperm quality traits in endangered golden mahseer, Tor putitora (Hamilton, 1822).
34757130	0	43	theme	testicular	46:55	arg1	genes					91:95	antioxidative defence genes	69:95	antioxidative defence genes	69:95	Dietary β-glucan influences the expression of testicular aquaporins, antioxidative defence genes and sperm quality traits in endangered golden mahseer, Tor putitora (Hamilton, 1822).
34757130	0	43	theme	testicular	46:55	arg1	aquaporins					57:66	testicular aquaporins	46:66	testicular aquaporins	46:66	Dietary β-glucan influences the expression of testicular aquaporins, antioxidative defence genes and sperm quality traits in endangered golden mahseer, Tor putitora (Hamilton, 1822).
34757130	8	44	theme	reactive	1275:1282	arg1	species					1291:1297	reactive oxygen species	1275:1297	reactive oxygen species	1275:1297	On the other hand, the higher seminal plasma total antioxidant capacity observed in 0.5 and 1% β-glucan fed brooders indicated increased scavenging ability of reactive oxygen species.
34757130	1	45	theme	dietary	197:203	arg1	β-glucan					205:212	dietary β-glucan	197:212	dietary β-glucan	197:212	The effect of dietary β-glucan on seminal plasma composition, sperm characteristics, expression of aquaporins, and antioxidative defence genes of golden mahseer was evaluated.
34757130	8	46	located	observed	1188:1195	arg2	capacity					1179:1186	the higher seminal plasma total antioxidant capacity	1135:1186	the higher seminal plasma total antioxidant capacity observed in 0.5 and 1% β-glucan fed brooders	1135:1231	On the other hand, the higher seminal plasma total antioxidant capacity observed in 0.5 and 1% β-glucan fed brooders indicated increased scavenging ability of reactive oxygen species.
34757130	8	46	located	observed	1188:1195	arg1	brooders					1224:1231	0.5 and 1% β-glucan fed brooders	1200:1231	0.5 and 1% β-glucan fed brooders	1200:1231	On the other hand, the higher seminal plasma total antioxidant capacity observed in 0.5 and 1% β-glucan fed brooders indicated increased scavenging ability of reactive oxygen species.
34757130	8	47	theme	species	1291:1297	arg1	ability					1264:1270	increased scavenging ability	1243:1270	increased scavenging ability of reactive oxygen species	1243:1297	On the other hand, the higher seminal plasma total antioxidant capacity observed in 0.5 and 1% β-glucan fed brooders indicated increased scavenging ability of reactive oxygen species.
34757130	10	48	theme	mahseer	1578:1584	arg1	broodstock					1557:1566	quality broodstock	1549:1566	quality broodstock of golden mahseer	1549:1584	Collectively, dietary β-glucan (0.5%) can be a practical approach to developing quality broodstock of golden mahseer.
34757130	5	49	theme	marked	700:705	arg1	down-regulation					707:721	The marked down-regulation	696:721	The marked down-regulation in the transcript abundance of testicular aqp3a noticed in 1.5% β-glucan fed brooders	696:807	The marked down-regulation in the transcript abundance of testicular aqp3a noticed in 1.5% β-glucan fed brooders corresponds to their poor sperm quality.
34757130	4	50	dep	sperm	572:576	arg1	count					578:582	count	578:582	count	578:582	sperm count, motility, viability, and morphology with no effect on gonadosomatic index and seminal plasma energy resources.
34757130	4	50	dep	sperm	572:576	arg1	viability					595:603	viability	595:603	viability	595:603	sperm count, motility, viability, and morphology with no effect on gonadosomatic index and seminal plasma energy resources.
34757130	4	50	dep	sperm	572:576	arg1	morphology					610:619	morphology	610:619	morphology	610:619	sperm count, motility, viability, and morphology with no effect on gonadosomatic index and seminal plasma energy resources.
34757130	4	50	dep	sperm	572:576	arg1	motility					585:592	motility	585:592	motility	585:592	sperm count, motility, viability, and morphology with no effect on gonadosomatic index and seminal plasma energy resources.
34757130	1	51	theme	plasma	225:230	arg1	composition					232:242	seminal plasma composition	217:242	seminal plasma composition	217:242	The effect of dietary β-glucan on seminal plasma composition, sperm characteristics, expression of aquaporins, and antioxidative defence genes of golden mahseer was evaluated.
34757130	7	52	theme	sod1	1110:1113	arg1	levels					1081:1086	relatively higher expression levels	1052:1086	relatively higher expression levels of testicular gst and sod1	1052:1113	In contrast, control and higher β-glucan (1 and 1.5%) groups displayed relatively higher expression levels of testicular gst and sod1.
34757130	5	53	theme	%	785:785	arg1	brooders					800:807	1.5% β-glucan fed brooders	782:807	1.5% β-glucan fed brooders	782:807	The marked down-regulation in the transcript abundance of testicular aqp3a noticed in 1.5% β-glucan fed brooders corresponds to their poor sperm quality.
34757130	6	54	theme	genes	882:886	arg1	lowest					943:948	lowest	943:948	lowest	943:948	Further, the mRNA expression of genes encoding antioxidant enzymes, namely gst and sod1, was lowest in 0.5% β-glucan fed brooders.
34757130	6	54	theme	genes	882:886	arg1	expression					868:877	the mRNA expression	859:877	the mRNA expression of genes encoding antioxidant enzymes, namely gst and sod1,	859:937	Further, the mRNA expression of genes encoding antioxidant enzymes, namely gst and sod1, was lowest in 0.5% β-glucan fed brooders.
34757130	0	55	theme	endangered	125:134	arg1	mahseer					143:149	endangered golden mahseer	125:149	endangered golden mahseer	125:149	Dietary β-glucan influences the expression of testicular aquaporins, antioxidative defence genes and sperm quality traits in endangered golden mahseer, Tor putitora (Hamilton, 1822).
34757130	0	56	from	genes	91:95	arg1	mahseer					143:149	endangered golden mahseer	125:149	endangered golden mahseer	125:149	Dietary β-glucan influences the expression of testicular aquaporins, antioxidative defence genes and sperm quality traits in endangered golden mahseer, Tor putitora (Hamilton, 1822).
34757130	3	57	theme	β-glucan	514:521	arg1	Feeding					498:504	Feeding	498:504	Feeding of 0.5% β-glucan	498:521	Feeding of 0.5% β-glucan was found to improve sperm characteristics, viz.
34757130	9	58	theme	%	1331:1331	arg1	β-glucan					1333:1340	0.5% β-glucan	1328:1340	0.5% β-glucan	1328:1340	Overall, supplementation of 0.5% β-glucan improved sperm quality and antioxidative potential, but the higher inclusion (1.5%) negatively affected sperm characteristics.
34757130	2	59	theme	golden	460:465	arg1	brooders					475:482	male golden mahseer brooders	455:482	male golden mahseer brooders	455:482	For that, four experimental diets containing 0 (control), 0.5, 1, and 1.5% β-glucan were fed to male golden mahseer brooders for 130 days.
34757130	7	60	theme	higher	1063:1068	arg1	levels					1081:1086	relatively higher expression levels	1052:1086	relatively higher expression levels of testicular gst and sod1	1052:1113	In contrast, control and higher β-glucan (1 and 1.5%) groups displayed relatively higher expression levels of testicular gst and sod1.
34757130	3	61	theme	0.5	509:511	arg1	%					512:512	%	512:512	%	512:512	Feeding of 0.5% β-glucan was found to improve sperm characteristics, viz.
34757130	0	62	from	traits	115:120	arg1	mahseer					143:149	endangered golden mahseer	125:149	endangered golden mahseer	125:149	Dietary β-glucan influences the expression of testicular aquaporins, antioxidative defence genes and sperm quality traits in endangered golden mahseer, Tor putitora (Hamilton, 1822).
34757130	5	63	theme	sperm	835:839	arg1	quality					841:847	their poor sperm quality	824:847	their poor sperm quality	824:847	The marked down-regulation in the transcript abundance of testicular aqp3a noticed in 1.5% β-glucan fed brooders corresponds to their poor sperm quality.
34757130	8	64	theme	seminal	1146:1152	arg1	capacity					1179:1186	the higher seminal plasma total antioxidant capacity	1135:1186	the higher seminal plasma total antioxidant capacity observed in 0.5 and 1% β-glucan fed brooders	1135:1231	On the other hand, the higher seminal plasma total antioxidant capacity observed in 0.5 and 1% β-glucan fed brooders indicated increased scavenging ability of reactive oxygen species.
34757130	7	65	theme	testicular	1091:1100	arg1	gst					1102:1104	testicular gst	1091:1104	testicular gst	1091:1104	In contrast, control and higher β-glucan (1 and 1.5%) groups displayed relatively higher expression levels of testicular gst and sod1.
34757130	9	66	theme	antioxidative	1369:1381	arg1	potential					1383:1391	antioxidative potential	1369:1391	antioxidative potential	1369:1391	Overall, supplementation of 0.5% β-glucan improved sperm quality and antioxidative potential, but the higher inclusion (1.5%) negatively affected sperm characteristics.
34757130	8	67	theme	total	1161:1165	arg1	capacity					1179:1186	the higher seminal plasma total antioxidant capacity	1135:1186	the higher seminal plasma total antioxidant capacity observed in 0.5 and 1% β-glucan fed brooders	1135:1231	On the other hand, the higher seminal plasma total antioxidant capacity observed in 0.5 and 1% β-glucan fed brooders indicated increased scavenging ability of reactive oxygen species.
34757130	5	68	from	down-regulation	707:721	arg1	abundance					741:749	the transcript abundance	726:749	the transcript abundance of testicular aqp3a noticed in 1.5% β-glucan fed brooders	726:807	The marked down-regulation in the transcript abundance of testicular aqp3a noticed in 1.5% β-glucan fed brooders corresponds to their poor sperm quality.
34757130	0	69	theme	sperm	101:105	arg1	aquaporins					57:66	testicular aquaporins	46:66	testicular aquaporins	46:66	Dietary β-glucan influences the expression of testicular aquaporins, antioxidative defence genes and sperm quality traits in endangered golden mahseer, Tor putitora (Hamilton, 1822).
34757130	0	69	theme	sperm	101:105	arg1	traits					115:120	sperm quality traits	101:120	sperm quality traits	101:120	Dietary β-glucan influences the expression of testicular aquaporins, antioxidative defence genes and sperm quality traits in endangered golden mahseer, Tor putitora (Hamilton, 1822).
34757130	1	70	theme	golden	329:334	arg1	mahseer					336:342	golden mahseer	329:342	golden mahseer	329:342	The effect of dietary β-glucan on seminal plasma composition, sperm characteristics, expression of aquaporins, and antioxidative defence genes of golden mahseer was evaluated.
34757130	6	71	theme	antioxidant	897:907	arg1	enzymes					909:915	antioxidant enzymes	897:915	antioxidant enzymes	897:915	Further, the mRNA expression of genes encoding antioxidant enzymes, namely gst and sod1, was lowest in 0.5% β-glucan fed brooders.
34757130	4	72	theme	seminal	663:669	arg1	resources					685:693	seminal plasma energy resources	663:693	seminal plasma energy resources	663:693	sperm count, motility, viability, and morphology with no effect on gonadosomatic index and seminal plasma energy resources.
34757130	5	73	theme	testicular	754:763	arg1	aqp3a					765:769	testicular aqp3a	754:769	testicular aqp3a noticed in 1.5% β-glucan fed brooders	754:807	The marked down-regulation in the transcript abundance of testicular aqp3a noticed in 1.5% β-glucan fed brooders corresponds to their poor sperm quality.
34757130	6	74	theme	fed	967:969	arg1	brooders					971:978	0.5% β-glucan fed brooders	953:978	0.5% β-glucan fed brooders	953:978	Further, the mRNA expression of genes encoding antioxidant enzymes, namely gst and sod1, was lowest in 0.5% β-glucan fed brooders.
34757130	4	75	theme	energy	678:683	arg1	resources					685:693	seminal plasma energy resources	663:693	seminal plasma energy resources	663:693	sperm count, motility, viability, and morphology with no effect on gonadosomatic index and seminal plasma energy resources.
34757130	6	76	theme	%	956:956	arg1	brooders					971:978	0.5% β-glucan fed brooders	953:978	0.5% β-glucan fed brooders	953:978	Further, the mRNA expression of genes encoding antioxidant enzymes, namely gst and sod1, was lowest in 0.5% β-glucan fed brooders.
34757130	5	77	theme	transcript	730:739	arg1	abundance					741:749	the transcript abundance	726:749	the transcript abundance of testicular aqp3a noticed in 1.5% β-glucan fed brooders	726:807	The marked down-regulation in the transcript abundance of testicular aqp3a noticed in 1.5% β-glucan fed brooders corresponds to their poor sperm quality.
34757130	8	78	theme	fed	1220:1222	arg1	brooders					1224:1231	0.5 and 1% β-glucan fed brooders	1200:1231	0.5 and 1% β-glucan fed brooders	1200:1231	On the other hand, the higher seminal plasma total antioxidant capacity observed in 0.5 and 1% β-glucan fed brooders indicated increased scavenging ability of reactive oxygen species.
34757130	9	79	theme	sperm	1351:1355	arg1	quality					1357:1363	sperm quality	1351:1363	sperm quality	1351:1363	Overall, supplementation of 0.5% β-glucan improved sperm quality and antioxidative potential, but the higher inclusion (1.5%) negatively affected sperm characteristics.
34757130	5	80	theme	aqp3a	765:769	arg1	abundance					741:749	the transcript abundance	726:749	the transcript abundance of testicular aqp3a noticed in 1.5% β-glucan fed brooders	726:807	The marked down-regulation in the transcript abundance of testicular aqp3a noticed in 1.5% β-glucan fed brooders corresponds to their poor sperm quality.
34757130	2	81	theme	experimental	374:385	arg1	diets					387:391	four experimental diets	369:391	four experimental diets containing 0 (control), 0.5, 1, and 1.5% β-glucan	369:441	For that, four experimental diets containing 0 (control), 0.5, 1, and 1.5% β-glucan were fed to male golden mahseer brooders for 130 days.
34757130	0	82	theme	aquaporins	57:66	arg1	expression					32:41	the expression	28:41	the expression of testicular aquaporins, antioxidative defence genes and sperm quality traits in endangered golden mahseer, Tor putitora (Hamilton, 1822)	28:180	Dietary β-glucan influences the expression of testicular aquaporins, antioxidative defence genes and sperm quality traits in endangered golden mahseer, Tor putitora (Hamilton, 1822).
34757130	8	83	theme	scavenging	1253:1262	arg1	ability					1264:1270	increased scavenging ability	1243:1270	increased scavenging ability of reactive oxygen species	1243:1297	On the other hand, the higher seminal plasma total antioxidant capacity observed in 0.5 and 1% β-glucan fed brooders indicated increased scavenging ability of reactive oxygen species.
34757130	4	84	from	effect	629:634	arg1	index					653:657	gonadosomatic index	639:657	gonadosomatic index	639:657	sperm count, motility, viability, and morphology with no effect on gonadosomatic index and seminal plasma energy resources.
34757130	4	84	from	effect	629:634	arg1	resources					685:693	seminal plasma energy resources	663:693	seminal plasma energy resources	663:693	sperm count, motility, viability, and morphology with no effect on gonadosomatic index and seminal plasma energy resources.
34757130	1	85	theme	antioxidative	298:310	arg1	genes					320:324	antioxidative defence genes	298:324	antioxidative defence genes of golden mahseer	298:342	The effect of dietary β-glucan on seminal plasma composition, sperm characteristics, expression of aquaporins, and antioxidative defence genes of golden mahseer was evaluated.
34757130	1	86	theme	β-glucan	205:212	arg1	effect					187:192	The effect	183:192	The effect of dietary β-glucan on seminal plasma composition, sperm characteristics, expression of aquaporins, and antioxidative defence genes of golden mahseer	183:342	The effect of dietary β-glucan on seminal plasma composition, sperm characteristics, expression of aquaporins, and antioxidative defence genes of golden mahseer was evaluated.
34757130	8	87	theme	oxygen	1284:1289	arg1	species					1291:1297	reactive oxygen species	1275:1297	reactive oxygen species	1275:1297	On the other hand, the higher seminal plasma total antioxidant capacity observed in 0.5 and 1% β-glucan fed brooders indicated increased scavenging ability of reactive oxygen species.
34757130	10	88	theme	golden	1571:1576	arg1	mahseer					1578:1584	golden mahseer	1571:1584	golden mahseer	1571:1584	Collectively, dietary β-glucan (0.5%) can be a practical approach to developing quality broodstock of golden mahseer.
34757130	1	89	theme	seminal	217:223	arg1	composition					232:242	seminal plasma composition	217:242	seminal plasma composition	217:242	The effect of dietary β-glucan on seminal plasma composition, sperm characteristics, expression of aquaporins, and antioxidative defence genes of golden mahseer was evaluated.
34190400	4	0	theme	backing	794:800	arg1	electrospun					730:740	An electrospun poly(caprolactone) (PCL)	727:765	An electrospun poly(caprolactone) (PCL)	727:765	An electrospun poly(caprolactone) (PCL) was used as an impermeable backing layer to protect the mucoadhesive component from tongue movement and drug loss.
34190400	4	0	theme	backing	794:800	arg1	layer					802:806	an impermeable backing layer	779:806	an impermeable backing layer	779:806	An electrospun poly(caprolactone) (PCL) was used as an impermeable backing layer to protect the mucoadhesive component from tongue movement and drug loss.
34190400	3	1	theme	vinylalcohol	546:557	arg1	/ibuprofen					565:574	chitosan/poly(vinylalcohol) (PVA)/ibuprofen	532:574	chitosan/poly(vinylalcohol) (PVA)/ibuprofen	532:574	The patches were fabricated through simultaneous electrospinning of chitosan/poly(vinylalcohol) (PVA)/ibuprofen and electrospraying of phenylalanine amino acid nanotubes (PhNTs) containing metronidazole into the electrospun mats through a layer-by-layer process.
34190400	1	2	theme	periodontal	258:268	arg1	disease					270:276	periodontal disease	258:276	periodontal disease	258:276	Mucoadhesive buccal patch is a promising dosage form for a successful oral drug delivery, which provides unique advantages for various applications such as treatment of periodontal disease and postdental surgery disorders.
34190400	9	3	theme	polymers	1558:1565	arg1	amount					1535:1540	an optimal amount	1524:1540	an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g)	1524:1618	The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria.
34190400	9	3	theme	polymers	1558:1565	arg1	polymers					1558:1565	mucoadhesive polymers	1545:1565	mucoadhesive polymers (PVA/chitosan 80:20)	1545:1586	The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria.
34190400	9	3	theme	polymers	1558:1565	arg1	0.05 g					1612:1617	0.05 g	1612:1617	0.05 g	1612:1617	The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria.
34190400	9	3	theme	polymers	1558:1565	arg1	agent					1605:1609	crosslinking agent	1592:1609	crosslinking agent (0.05 g)	1592:1618	The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria.
34190400	9	3	theme	polymers	1558:1565	arg1	PVA/chitosan					1568:1579	PVA/chitosan	1568:1579	PVA/chitosan	1568:1579	The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria.
34190400	6	4	theme	patches	1196:1202	arg1	index					1183:1187	The swelling index	1170:1187	The swelling index of the patches	1170:1202	The swelling index of the patches was examined with respect to the PVA/chitosan ratio.
34190400	9	5	theme	agent	1605:1609	arg1	amount					1535:1540	an optimal amount	1524:1540	an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g)	1524:1618	The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria.
34190400	9	5	theme	agent	1605:1609	arg1	polymers					1558:1565	mucoadhesive polymers	1545:1565	mucoadhesive polymers (PVA/chitosan 80:20)	1545:1586	The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria.
34190400	9	5	theme	agent	1605:1609	arg1	0.05 g					1612:1617	0.05 g	1612:1617	0.05 g	1612:1617	The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria.
34190400	9	5	theme	agent	1605:1609	arg1	agent					1605:1609	crosslinking agent	1592:1609	crosslinking agent (0.05 g)	1592:1618	The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria.
34190400	9	5	theme	agent	1605:1609	arg1	PVA/chitosan					1568:1579	PVA/chitosan	1568:1579	PVA/chitosan	1568:1579	The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria.
34190400	10	6	theme	novel	1792:1796	arg1	composition					1798:1808	a novel composition	1790:1808	a novel composition	1790:1808	The synthesized multifunctional mucoadhesive patch with a novel composition and design has a great potential for oral therapeutic applications.
34190400	4	7	theme	drug	871:874	arg1	loss					876:879	drug loss	871:879	drug loss	871:879	An electrospun poly(caprolactone) (PCL) was used as an impermeable backing layer to protect the mucoadhesive component from tongue movement and drug loss.
34190400	6	8	theme	swelling	1174:1181	arg1	index					1183:1187	The swelling index	1170:1187	The swelling index of the patches	1170:1202	The swelling index of the patches was examined with respect to the PVA/chitosan ratio.
34190400	3	9	theme	amino	613:617	arg1	PhNTs					635:639	PhNTs	635:639	PhNTs	635:639	The patches were fabricated through simultaneous electrospinning of chitosan/poly(vinylalcohol) (PVA)/ibuprofen and electrospraying of phenylalanine amino acid nanotubes (PhNTs) containing metronidazole into the electrospun mats through a layer-by-layer process.
34190400	3	9	theme	amino	613:617	arg1	nanotubes					624:632	phenylalanine amino acid nanotubes	599:632	phenylalanine amino acid nanotubes (PhNTs) containing metronidazole into the electrospun mats through a layer-by-layer process	599:724	The patches were fabricated through simultaneous electrospinning of chitosan/poly(vinylalcohol) (PVA)/ibuprofen and electrospraying of phenylalanine amino acid nanotubes (PhNTs) containing metronidazole into the electrospun mats through a layer-by-layer process.
34190400	8	10	theme	cell	1382:1385	arg1	viability					1387:1395	The cell viability	1378:1395	The cell viability of buccal patches	1378:1413	The cell viability of buccal patches was assessed by methylthiazolydiphenyl-tetrazolium bromide test on L929 fibroblast cell line.
34190400	1	11	theme	dosage	130:135	arg1	form					137:140	a promising dosage form	118:140	a promising dosage form	118:140	Mucoadhesive buccal patch is a promising dosage form for a successful oral drug delivery, which provides unique advantages for various applications such as treatment of periodontal disease and postdental surgery disorders.
34190400	1	11	theme	dosage	130:135	arg1	patch					109:113	Mucoadhesive buccal patch	89:113	Mucoadhesive buccal patch	89:113	Mucoadhesive buccal patch is a promising dosage form for a successful oral drug delivery, which provides unique advantages for various applications such as treatment of periodontal disease and postdental surgery disorders.
34190400	8	12	theme	L929	1482:1485	arg1	line					1503:1506	L929 fibroblast cell line	1482:1506	L929 fibroblast cell line	1482:1506	The cell viability of buccal patches was assessed by methylthiazolydiphenyl-tetrazolium bromide test on L929 fibroblast cell line.
34190400	1	13	theme	various	216:222	arg1	applications					224:235	various applications	216:235	various applications such as treatment of periodontal disease and postdental surgery disorders	216:309	Mucoadhesive buccal patch is a promising dosage form for a successful oral drug delivery, which provides unique advantages for various applications such as treatment of periodontal disease and postdental surgery disorders.
34190400	1	13	theme	various	216:222	arg1	treatment					245:253	treatment	245:253	treatment of periodontal disease and postdental surgery disorders	245:309	Mucoadhesive buccal patch is a promising dosage form for a successful oral drug delivery, which provides unique advantages for various applications such as treatment of periodontal disease and postdental surgery disorders.
34190400	8	14	theme	cell	1498:1501	arg1	line					1503:1506	L929 fibroblast cell line	1482:1506	L929 fibroblast cell line	1482:1506	The cell viability of buccal patches was assessed by methylthiazolydiphenyl-tetrazolium bromide test on L929 fibroblast cell line.
34190400	3	15	theme	nanotubes	624:632	arg1	/ibuprofen					565:574	chitosan/poly(vinylalcohol) (PVA)/ibuprofen	532:574	chitosan/poly(vinylalcohol) (PVA)/ibuprofen	532:574	The patches were fabricated through simultaneous electrospinning of chitosan/poly(vinylalcohol) (PVA)/ibuprofen and electrospraying of phenylalanine amino acid nanotubes (PhNTs) containing metronidazole into the electrospun mats through a layer-by-layer process.
34190400	3	15	theme	nanotubes	624:632	arg1	electrospraying					580:594	electrospraying	580:594	electrospraying of phenylalanine amino acid nanotubes (PhNTs) containing metronidazole into the electrospun mats through a layer-by-layer process	580:724	The patches were fabricated through simultaneous electrospinning of chitosan/poly(vinylalcohol) (PVA)/ibuprofen and electrospraying of phenylalanine amino acid nanotubes (PhNTs) containing metronidazole into the electrospun mats through a layer-by-layer process.
34190400	9	16	theme	optimal	1527:1533	arg1	amount					1535:1540	an optimal amount	1524:1540	an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g)	1524:1618	The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria.
34190400	9	16	theme	optimal	1527:1533	arg1	polymers					1558:1565	mucoadhesive polymers	1545:1565	mucoadhesive polymers (PVA/chitosan 80:20)	1545:1586	The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria.
34190400	9	16	theme	optimal	1527:1533	arg1	0.05 g					1612:1617	0.05 g	1612:1617	0.05 g	1612:1617	The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria.
34190400	9	16	theme	optimal	1527:1533	arg1	agent					1605:1609	crosslinking agent	1592:1609	crosslinking agent (0.05 g)	1592:1618	The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria.
34190400	9	16	theme	optimal	1527:1533	arg1	PVA/chitosan					1568:1579	PVA/chitosan	1568:1579	PVA/chitosan	1568:1579	The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria.
34190400	10	17	theme	great	1827:1831	arg1	potential					1833:1841	a great potential	1825:1841	a great potential for oral therapeutic applications	1825:1875	The synthesized multifunctional mucoadhesive patch with a novel composition and design has a great potential for oral therapeutic applications.
34190400	7	18	theme	swelling	1357:1364	arg1	properties					1366:1375	mucoadhesive and swelling properties	1340:1375	mucoadhesive and swelling properties	1340:1375	The effect of genipin addition to the electrospinning solution was also studied on mucoadhesive and swelling properties.
34190400	1	19	theme	successful	148:157	arg1	delivery					169:176	a successful oral drug delivery	146:176	a successful oral drug delivery	146:176	Mucoadhesive buccal patch is a promising dosage form for a successful oral drug delivery, which provides unique advantages for various applications such as treatment of periodontal disease and postdental surgery disorders.
34190400	0	20	theme	delivery	66:73	arg1	applications					75:86	drug delivery applications	61:86	drug delivery applications	61:86	Fabrication of multifunctional mucoadhesive buccal patch for drug delivery applications.
34190400	5	21	theme	scanning	922:929	arg1	SEM					952:954	SEM	952:954	SEM	952:954	Buccal patches were characterized using scanning electron microscopy (SEM) and field emission scanning electron microscopy (FESEM) and also evaluated in terms of physicomechanical parameters such as pH, weight, thickness, tensile strength, folding endurance, and mucoadhesive properties.
34190400	5	21	theme	scanning	922:929	arg1	microscopy					940:949	scanning electron microscopy	922:949	scanning electron microscopy (SEM)	922:955	Buccal patches were characterized using scanning electron microscopy (SEM) and field emission scanning electron microscopy (FESEM) and also evaluated in terms of physicomechanical parameters such as pH, weight, thickness, tensile strength, folding endurance, and mucoadhesive properties.
34190400	1	22	theme	drug	164:167	arg1	delivery					169:176	a successful oral drug delivery	146:176	a successful oral drug delivery	146:176	Mucoadhesive buccal patch is a promising dosage form for a successful oral drug delivery, which provides unique advantages for various applications such as treatment of periodontal disease and postdental surgery disorders.
34190400	4	23	theme	impermeable	782:792	arg1	electrospun					730:740	An electrospun poly(caprolactone) (PCL)	727:765	An electrospun poly(caprolactone) (PCL)	727:765	An electrospun poly(caprolactone) (PCL) was used as an impermeable backing layer to protect the mucoadhesive component from tongue movement and drug loss.
34190400	4	23	theme	impermeable	782:792	arg1	layer					802:806	an impermeable backing layer	779:806	an impermeable backing layer	779:806	An electrospun poly(caprolactone) (PCL) was used as an impermeable backing layer to protect the mucoadhesive component from tongue movement and drug loss.
34190400	5	24	theme	tensile	1104:1110	arg1	strength					1112:1119	tensile strength	1104:1119	tensile strength	1104:1119	Buccal patches were characterized using scanning electron microscopy (SEM) and field emission scanning electron microscopy (FESEM) and also evaluated in terms of physicomechanical parameters such as pH, weight, thickness, tensile strength, folding endurance, and mucoadhesive properties.
34190400	1	25	theme	Mucoadhesive	89:100	arg1	form					137:140	a promising dosage form	118:140	a promising dosage form	118:140	Mucoadhesive buccal patch is a promising dosage form for a successful oral drug delivery, which provides unique advantages for various applications such as treatment of periodontal disease and postdental surgery disorders.
34190400	1	25	theme	Mucoadhesive	89:100	arg1	patch					109:113	Mucoadhesive buccal patch	89:113	Mucoadhesive buccal patch	89:113	Mucoadhesive buccal patch is a promising dosage form for a successful oral drug delivery, which provides unique advantages for various applications such as treatment of periodontal disease and postdental surgery disorders.
34190400	1	26	theme	postdental	282:291	arg1	surgery					293:299	postdental surgery	282:299	postdental surgery	282:299	Mucoadhesive buccal patch is a promising dosage form for a successful oral drug delivery, which provides unique advantages for various applications such as treatment of periodontal disease and postdental surgery disorders.
34190400	9	27	with	patch	1513:1517	arg1	amount					1535:1540	an optimal amount	1524:1540	an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g)	1524:1618	The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria.
34190400	9	27	with	patch	1513:1517	arg1	polymers					1558:1565	mucoadhesive polymers	1545:1565	mucoadhesive polymers (PVA/chitosan 80:20)	1545:1586	The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria.
34190400	9	27	with	patch	1513:1517	arg1	0.05 g					1612:1617	0.05 g	1612:1617	0.05 g	1612:1617	The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria.
34190400	9	27	with	patch	1513:1517	arg1	agent					1605:1609	crosslinking agent	1592:1609	crosslinking agent (0.05 g)	1592:1618	The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria.
34190400	9	27	with	patch	1513:1517	arg1	PVA/chitosan					1568:1579	PVA/chitosan	1568:1579	PVA/chitosan	1568:1579	The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria.
34190400	8	28	theme	buccal	1400:1405	arg1	patches					1407:1413	buccal patches	1400:1413	buccal patches	1400:1413	The cell viability of buccal patches was assessed by methylthiazolydiphenyl-tetrazolium bromide test on L929 fibroblast cell line.
34190400	10	29	theme	synthesized	1738:1748	arg1	patch					1779:1783	The synthesized multifunctional mucoadhesive patch	1734:1783	The synthesized multifunctional mucoadhesive patch with a novel composition and design	1734:1819	The synthesized multifunctional mucoadhesive patch with a novel composition and design has a great potential for oral therapeutic applications.
34190400	0	30	theme	multifunctional	15:29	arg1	patch					51:55	multifunctional mucoadhesive buccal patch	15:55	multifunctional mucoadhesive buccal patch	15:55	Fabrication of multifunctional mucoadhesive buccal patch for drug delivery applications.
34190400	5	31	theme	electron	985:992	arg1	FESEM					1006:1010	FESEM	1006:1010	FESEM	1006:1010	Buccal patches were characterized using scanning electron microscopy (SEM) and field emission scanning electron microscopy (FESEM) and also evaluated in terms of physicomechanical parameters such as pH, weight, thickness, tensile strength, folding endurance, and mucoadhesive properties.
34190400	5	31	theme	electron	985:992	arg1	microscopy					994:1003	scanning electron microscopy	976:1003	scanning electron microscopy (FESEM)	976:1011	Buccal patches were characterized using scanning electron microscopy (SEM) and field emission scanning electron microscopy (FESEM) and also evaluated in terms of physicomechanical parameters such as pH, weight, thickness, tensile strength, folding endurance, and mucoadhesive properties.
34190400	2	32	theme	reservoir	417:425	arg1	design					427:432	a multilayer reservoir design	404:432	a multilayer reservoir design	404:432	The aim of this study is to synthesize a novel multifunctional mucoadhesive buccal patch in a multilayer reservoir design for therapeutic applications.
34190400	0	33	theme	buccal	44:49	arg1	patch					51:55	multifunctional mucoadhesive buccal patch	15:55	multifunctional mucoadhesive buccal patch	15:55	Fabrication of multifunctional mucoadhesive buccal patch for drug delivery applications.
34190400	2	34	from	patch	395:399	arg1	design					427:432	a multilayer reservoir design	404:432	a multilayer reservoir design	404:432	The aim of this study is to synthesize a novel multifunctional mucoadhesive buccal patch in a multilayer reservoir design for therapeutic applications.
34190400	3	35	theme	electrospun	676:686	arg1	mats					688:691	the electrospun mats	672:691	the electrospun mats	672:691	The patches were fabricated through simultaneous electrospinning of chitosan/poly(vinylalcohol) (PVA)/ibuprofen and electrospraying of phenylalanine amino acid nanotubes (PhNTs) containing metronidazole into the electrospun mats through a layer-by-layer process.
34190400	9	36	theme	hemostatic	1639:1648	arg1	activity					1650:1657	an ideal hemostatic activity	1630:1657	an ideal hemostatic activity	1630:1657	The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria.
34190400	8	37	theme	bromide	1466:1472	arg1	test					1474:1477	methylthiazolydiphenyl-tetrazolium bromide test	1431:1477	methylthiazolydiphenyl-tetrazolium bromide test on L929 fibroblast cell line	1431:1506	The cell viability of buccal patches was assessed by methylthiazolydiphenyl-tetrazolium bromide test on L929 fibroblast cell line.
34190400	3	38	theme	simultaneous	500:511	arg1	electrospinning					513:527	simultaneous electrospinning	500:527	simultaneous electrospinning of chitosan/poly(vinylalcohol) (PVA)/ibuprofen and electrospraying of phenylalanine amino acid nanotubes (PhNTs) containing metronidazole into the electrospun mats through a layer-by-layer process	500:724	The patches were fabricated through simultaneous electrospinning of chitosan/poly(vinylalcohol) (PVA)/ibuprofen and electrospraying of phenylalanine amino acid nanotubes (PhNTs) containing metronidazole into the electrospun mats through a layer-by-layer process.
34190400	7	39	theme	addition	1279:1286	arg1	effect					1261:1266	The effect	1257:1266	The effect of genipin addition to the electrospinning solution	1257:1318	The effect of genipin addition to the electrospinning solution was also studied on mucoadhesive and swelling properties.
34190400	3	40	theme	layer-by-layer	703:716	arg1	process					718:724	a layer-by-layer process	701:724	a layer-by-layer process	701:724	The patches were fabricated through simultaneous electrospinning of chitosan/poly(vinylalcohol) (PVA)/ibuprofen and electrospraying of phenylalanine amino acid nanotubes (PhNTs) containing metronidazole into the electrospun mats through a layer-by-layer process.
34190400	4	41	theme	mucoadhesive	823:834	arg1	component					836:844	the mucoadhesive component	819:844	the mucoadhesive component	819:844	An electrospun poly(caprolactone) (PCL) was used as an impermeable backing layer to protect the mucoadhesive component from tongue movement and drug loss.
34190400	2	42	theme	mucoadhesive	375:386	arg1	patch					395:399	a novel multifunctional mucoadhesive buccal patch	351:399	a novel multifunctional mucoadhesive buccal patch in a multilayer reservoir design for therapeutic applications	351:461	The aim of this study is to synthesize a novel multifunctional mucoadhesive buccal patch in a multilayer reservoir design for therapeutic applications.
34190400	9	43	theme	antibacterial	1670:1682	arg1	properties					1684:1693	antibacterial properties	1670:1693	antibacterial properties against Streptococcus mutans bacteria	1670:1731	The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria.
34190400	2	44	theme	novel	353:357	arg1	patch					395:399	a novel multifunctional mucoadhesive buccal patch	351:399	a novel multifunctional mucoadhesive buccal patch in a multilayer reservoir design for therapeutic applications	351:461	The aim of this study is to synthesize a novel multifunctional mucoadhesive buccal patch in a multilayer reservoir design for therapeutic applications.
34190400	5	45	theme	physicomechanical	1044:1060	arg1	pH					1081:1082	pH	1081:1082	pH	1081:1082	Buccal patches were characterized using scanning electron microscopy (SEM) and field emission scanning electron microscopy (FESEM) and also evaluated in terms of physicomechanical parameters such as pH, weight, thickness, tensile strength, folding endurance, and mucoadhesive properties.
34190400	5	45	theme	physicomechanical	1044:1060	arg1	strength					1112:1119	tensile strength	1104:1119	tensile strength	1104:1119	Buccal patches were characterized using scanning electron microscopy (SEM) and field emission scanning electron microscopy (FESEM) and also evaluated in terms of physicomechanical parameters such as pH, weight, thickness, tensile strength, folding endurance, and mucoadhesive properties.
34190400	5	45	theme	physicomechanical	1044:1060	arg1	properties					1158:1167	mucoadhesive properties	1145:1167	mucoadhesive properties	1145:1167	Buccal patches were characterized using scanning electron microscopy (SEM) and field emission scanning electron microscopy (FESEM) and also evaluated in terms of physicomechanical parameters such as pH, weight, thickness, tensile strength, folding endurance, and mucoadhesive properties.
34190400	5	45	theme	physicomechanical	1044:1060	arg1	weight					1085:1090	weight	1085:1090	weight	1085:1090	Buccal patches were characterized using scanning electron microscopy (SEM) and field emission scanning electron microscopy (FESEM) and also evaluated in terms of physicomechanical parameters such as pH, weight, thickness, tensile strength, folding endurance, and mucoadhesive properties.
34190400	5	45	theme	physicomechanical	1044:1060	arg1	parameters					1062:1071	physicomechanical parameters	1044:1071	physicomechanical parameters such as pH, weight, thickness, tensile strength, folding endurance, and mucoadhesive properties	1044:1167	Buccal patches were characterized using scanning electron microscopy (SEM) and field emission scanning electron microscopy (FESEM) and also evaluated in terms of physicomechanical parameters such as pH, weight, thickness, tensile strength, folding endurance, and mucoadhesive properties.
34190400	5	45	theme	physicomechanical	1044:1060	arg1	thickness					1093:1101	thickness	1093:1101	thickness	1093:1101	Buccal patches were characterized using scanning electron microscopy (SEM) and field emission scanning electron microscopy (FESEM) and also evaluated in terms of physicomechanical parameters such as pH, weight, thickness, tensile strength, folding endurance, and mucoadhesive properties.
34190400	5	45	theme	physicomechanical	1044:1060	arg1	endurance					1130:1138	folding endurance	1122:1138	folding endurance	1122:1138	Buccal patches were characterized using scanning electron microscopy (SEM) and field emission scanning electron microscopy (FESEM) and also evaluated in terms of physicomechanical parameters such as pH, weight, thickness, tensile strength, folding endurance, and mucoadhesive properties.
34190400	3	46	theme	/ibuprofen	565:574	arg1	electrospinning					513:527	simultaneous electrospinning	500:527	simultaneous electrospinning of chitosan/poly(vinylalcohol) (PVA)/ibuprofen and electrospraying of phenylalanine amino acid nanotubes (PhNTs) containing metronidazole into the electrospun mats through a layer-by-layer process	500:724	The patches were fabricated through simultaneous electrospinning of chitosan/poly(vinylalcohol) (PVA)/ibuprofen and electrospraying of phenylalanine amino acid nanotubes (PhNTs) containing metronidazole into the electrospun mats through a layer-by-layer process.
34190400	1	47	theme	disease	270:276	arg1	treatment					245:253	treatment	245:253	treatment of periodontal disease and postdental surgery disorders	245:309	Mucoadhesive buccal patch is a promising dosage form for a successful oral drug delivery, which provides unique advantages for various applications such as treatment of periodontal disease and postdental surgery disorders.
34190400	10	48	theme	therapeutic	1852:1862	arg1	applications					1864:1875	oral therapeutic applications	1847:1875	oral therapeutic applications	1847:1875	The synthesized multifunctional mucoadhesive patch with a novel composition and design has a great potential for oral therapeutic applications.
34190400	9	49	theme	mutans	1717:1722	arg1	bacteria					1724:1731	Streptococcus mutans bacteria	1703:1731	Streptococcus mutans bacteria	1703:1731	The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria.
34190400	5	50	theme	electron	931:938	arg1	SEM					952:954	SEM	952:954	SEM	952:954	Buccal patches were characterized using scanning electron microscopy (SEM) and field emission scanning electron microscopy (FESEM) and also evaluated in terms of physicomechanical parameters such as pH, weight, thickness, tensile strength, folding endurance, and mucoadhesive properties.
34190400	5	50	theme	electron	931:938	arg1	microscopy					940:949	scanning electron microscopy	922:949	scanning electron microscopy (SEM)	922:955	Buccal patches were characterized using scanning electron microscopy (SEM) and field emission scanning electron microscopy (FESEM) and also evaluated in terms of physicomechanical parameters such as pH, weight, thickness, tensile strength, folding endurance, and mucoadhesive properties.
34190400	3	51	theme	PVA	561:563	arg1	/ibuprofen					565:574	chitosan/poly(vinylalcohol) (PVA)/ibuprofen	532:574	chitosan/poly(vinylalcohol) (PVA)/ibuprofen	532:574	The patches were fabricated through simultaneous electrospinning of chitosan/poly(vinylalcohol) (PVA)/ibuprofen and electrospraying of phenylalanine amino acid nanotubes (PhNTs) containing metronidazole into the electrospun mats through a layer-by-layer process.
34190400	5	52	theme	Buccal	882:887	arg1	patches					889:895	Buccal patches	882:895	Buccal patches	882:895	Buccal patches were characterized using scanning electron microscopy (SEM) and field emission scanning electron microscopy (FESEM) and also evaluated in terms of physicomechanical parameters such as pH, weight, thickness, tensile strength, folding endurance, and mucoadhesive properties.
34190400	3	53	theme	phenylalanine	599:611	arg1	PhNTs					635:639	PhNTs	635:639	PhNTs	635:639	The patches were fabricated through simultaneous electrospinning of chitosan/poly(vinylalcohol) (PVA)/ibuprofen and electrospraying of phenylalanine amino acid nanotubes (PhNTs) containing metronidazole into the electrospun mats through a layer-by-layer process.
34190400	3	53	theme	phenylalanine	599:611	arg1	nanotubes					624:632	phenylalanine amino acid nanotubes	599:632	phenylalanine amino acid nanotubes (PhNTs) containing metronidazole into the electrospun mats through a layer-by-layer process	599:724	The patches were fabricated through simultaneous electrospinning of chitosan/poly(vinylalcohol) (PVA)/ibuprofen and electrospraying of phenylalanine amino acid nanotubes (PhNTs) containing metronidazole into the electrospun mats through a layer-by-layer process.
34190400	5	54	theme	parameters	1062:1071	arg1	terms					1035:1039	terms	1035:1039	terms of physicomechanical parameters such as pH, weight, thickness, tensile strength, folding endurance, and mucoadhesive properties	1035:1167	Buccal patches were characterized using scanning electron microscopy (SEM) and field emission scanning electron microscopy (FESEM) and also evaluated in terms of physicomechanical parameters such as pH, weight, thickness, tensile strength, folding endurance, and mucoadhesive properties.
34190400	3	55	theme	electrospraying	580:594	arg1	electrospinning					513:527	simultaneous electrospinning	500:527	simultaneous electrospinning of chitosan/poly(vinylalcohol) (PVA)/ibuprofen and electrospraying of phenylalanine amino acid nanotubes (PhNTs) containing metronidazole into the electrospun mats through a layer-by-layer process	500:724	The patches were fabricated through simultaneous electrospinning of chitosan/poly(vinylalcohol) (PVA)/ibuprofen and electrospraying of phenylalanine amino acid nanotubes (PhNTs) containing metronidazole into the electrospun mats through a layer-by-layer process.
34190400	8	56	from	test	1474:1477	arg1	line					1503:1506	L929 fibroblast cell line	1482:1506	L929 fibroblast cell line	1482:1506	The cell viability of buccal patches was assessed by methylthiazolydiphenyl-tetrazolium bromide test on L929 fibroblast cell line.
34190400	1	57	theme	unique	194:199	arg1	advantages					201:210	unique advantages	194:210	unique advantages	194:210	Mucoadhesive buccal patch is a promising dosage form for a successful oral drug delivery, which provides unique advantages for various applications such as treatment of periodontal disease and postdental surgery disorders.
34190400	10	58	theme	oral	1847:1850	arg1	applications					1864:1875	oral therapeutic applications	1847:1875	oral therapeutic applications	1847:1875	The synthesized multifunctional mucoadhesive patch with a novel composition and design has a great potential for oral therapeutic applications.
34190400	8	59	theme	fibroblast	1487:1496	arg1	line					1503:1506	L929 fibroblast cell line	1482:1506	L929 fibroblast cell line	1482:1506	The cell viability of buccal patches was assessed by methylthiazolydiphenyl-tetrazolium bromide test on L929 fibroblast cell line.
34190400	6	60	theme	PVA/chitosan	1237:1248	arg1	ratio					1250:1254	the PVA/chitosan ratio	1233:1254	the PVA/chitosan ratio	1233:1254	The swelling index of the patches was examined with respect to the PVA/chitosan ratio.
34190400	3	61	theme	acid	619:622	arg1	PhNTs					635:639	PhNTs	635:639	PhNTs	635:639	The patches were fabricated through simultaneous electrospinning of chitosan/poly(vinylalcohol) (PVA)/ibuprofen and electrospraying of phenylalanine amino acid nanotubes (PhNTs) containing metronidazole into the electrospun mats through a layer-by-layer process.
34190400	3	61	theme	acid	619:622	arg1	nanotubes					624:632	phenylalanine amino acid nanotubes	599:632	phenylalanine amino acid nanotubes (PhNTs) containing metronidazole into the electrospun mats through a layer-by-layer process	599:724	The patches were fabricated through simultaneous electrospinning of chitosan/poly(vinylalcohol) (PVA)/ibuprofen and electrospraying of phenylalanine amino acid nanotubes (PhNTs) containing metronidazole into the electrospun mats through a layer-by-layer process.
34190400	1	62	theme	promising	120:128	arg1	form					137:140	a promising dosage form	118:140	a promising dosage form	118:140	Mucoadhesive buccal patch is a promising dosage form for a successful oral drug delivery, which provides unique advantages for various applications such as treatment of periodontal disease and postdental surgery disorders.
34190400	1	62	theme	promising	120:128	arg1	patch					109:113	Mucoadhesive buccal patch	89:113	Mucoadhesive buccal patch	89:113	Mucoadhesive buccal patch is a promising dosage form for a successful oral drug delivery, which provides unique advantages for various applications such as treatment of periodontal disease and postdental surgery disorders.
34190400	1	63	dep	disease	270:276	arg1	disorders					301:309	disorders	301:309	disorders	301:309	Mucoadhesive buccal patch is a promising dosage form for a successful oral drug delivery, which provides unique advantages for various applications such as treatment of periodontal disease and postdental surgery disorders.
34190400	10	64	theme	mucoadhesive	1766:1777	arg1	patch					1779:1783	The synthesized multifunctional mucoadhesive patch	1734:1783	The synthesized multifunctional mucoadhesive patch with a novel composition and design	1734:1819	The synthesized multifunctional mucoadhesive patch with a novel composition and design has a great potential for oral therapeutic applications.
34190400	9	65	theme	mucoadhesive	1545:1556	arg1	polymers					1558:1565	mucoadhesive polymers	1545:1565	mucoadhesive polymers (PVA/chitosan 80:20)	1545:1586	The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria.
34190400	9	65	theme	mucoadhesive	1545:1556	arg1	PVA/chitosan					1568:1579	PVA/chitosan	1568:1579	PVA/chitosan	1568:1579	The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria.
34190400	10	66	contain	has	1821:1823	arg1	patch					1779:1783	The synthesized multifunctional mucoadhesive patch	1734:1783	The synthesized multifunctional mucoadhesive patch with a novel composition and design	1734:1819	The synthesized multifunctional mucoadhesive patch with a novel composition and design has a great potential for oral therapeutic applications.
34190400	10	66	contain	has	1821:1823	arg2	potential					1833:1841	a great potential	1825:1841	a great potential for oral therapeutic applications	1825:1875	The synthesized multifunctional mucoadhesive patch with a novel composition and design has a great potential for oral therapeutic applications.
34190400	1	67	theme	oral	159:162	arg1	delivery					169:176	a successful oral drug delivery	146:176	a successful oral drug delivery	146:176	Mucoadhesive buccal patch is a promising dosage form for a successful oral drug delivery, which provides unique advantages for various applications such as treatment of periodontal disease and postdental surgery disorders.
34190400	5	68	theme	mucoadhesive	1145:1156	arg1	properties					1158:1167	mucoadhesive properties	1145:1167	mucoadhesive properties	1145:1167	Buccal patches were characterized using scanning electron microscopy (SEM) and field emission scanning electron microscopy (FESEM) and also evaluated in terms of physicomechanical parameters such as pH, weight, thickness, tensile strength, folding endurance, and mucoadhesive properties.
34190400	10	69	theme	multifunctional	1750:1764	arg1	patch					1779:1783	The synthesized multifunctional mucoadhesive patch	1734:1783	The synthesized multifunctional mucoadhesive patch with a novel composition and design	1734:1819	The synthesized multifunctional mucoadhesive patch with a novel composition and design has a great potential for oral therapeutic applications.
34190400	4	70	dep	electrospun	730:740	arg1	poly					742:745	poly	742:745	An electrospun poly(caprolactone) (PCL)	727:765	An electrospun poly(caprolactone) (PCL) was used as an impermeable backing layer to protect the mucoadhesive component from tongue movement and drug loss.
34190400	4	70	dep	electrospun	730:740	arg1	caprolactone					747:758	caprolactone	747:758	caprolactone	747:758	An electrospun poly(caprolactone) (PCL) was used as an impermeable backing layer to protect the mucoadhesive component from tongue movement and drug loss.
34190400	4	70	dep	electrospun	730:740	arg1	PCL					762:764	PCL	762:764	PCL	762:764	An electrospun poly(caprolactone) (PCL) was used as an impermeable backing layer to protect the mucoadhesive component from tongue movement and drug loss.
34190400	7	71	theme	mucoadhesive	1340:1351	arg1	properties					1366:1375	mucoadhesive and swelling properties	1340:1375	mucoadhesive and swelling properties	1340:1375	The effect of genipin addition to the electrospinning solution was also studied on mucoadhesive and swelling properties.
34190400	5	72	theme	field	961:965	arg1	emission					967:974	field emission	961:974	field emission	961:974	Buccal patches were characterized using scanning electron microscopy (SEM) and field emission scanning electron microscopy (FESEM) and also evaluated in terms of physicomechanical parameters such as pH, weight, thickness, tensile strength, folding endurance, and mucoadhesive properties.
34190400	5	73	theme	folding	1122:1128	arg1	endurance					1130:1138	folding endurance	1122:1138	folding endurance	1122:1138	Buccal patches were characterized using scanning electron microscopy (SEM) and field emission scanning electron microscopy (FESEM) and also evaluated in terms of physicomechanical parameters such as pH, weight, thickness, tensile strength, folding endurance, and mucoadhesive properties.
34190400	1	74	theme	buccal	102:107	arg1	form					137:140	a promising dosage form	118:140	a promising dosage form	118:140	Mucoadhesive buccal patch is a promising dosage form for a successful oral drug delivery, which provides unique advantages for various applications such as treatment of periodontal disease and postdental surgery disorders.
34190400	1	74	theme	buccal	102:107	arg1	patch					109:113	Mucoadhesive buccal patch	89:113	Mucoadhesive buccal patch	89:113	Mucoadhesive buccal patch is a promising dosage form for a successful oral drug delivery, which provides unique advantages for various applications such as treatment of periodontal disease and postdental surgery disorders.
34190400	5	75	dep	microscopy	940:949	arg1	FESEM					1006:1010	FESEM	1006:1010	FESEM	1006:1010	Buccal patches were characterized using scanning electron microscopy (SEM) and field emission scanning electron microscopy (FESEM) and also evaluated in terms of physicomechanical parameters such as pH, weight, thickness, tensile strength, folding endurance, and mucoadhesive properties.
34190400	5	75	dep	microscopy	940:949	arg1	microscopy					994:1003	scanning electron microscopy	976:1003	scanning electron microscopy (FESEM)	976:1011	Buccal patches were characterized using scanning electron microscopy (SEM) and field emission scanning electron microscopy (FESEM) and also evaluated in terms of physicomechanical parameters such as pH, weight, thickness, tensile strength, folding endurance, and mucoadhesive properties.
34190400	2	76	theme	buccal	388:393	arg1	patch					395:399	a novel multifunctional mucoadhesive buccal patch	351:399	a novel multifunctional mucoadhesive buccal patch in a multilayer reservoir design for therapeutic applications	351:461	The aim of this study is to synthesize a novel multifunctional mucoadhesive buccal patch in a multilayer reservoir design for therapeutic applications.
34190400	0	77	theme	mucoadhesive	31:42	arg1	patch					51:55	multifunctional mucoadhesive buccal patch	15:55	multifunctional mucoadhesive buccal patch	15:55	Fabrication of multifunctional mucoadhesive buccal patch for drug delivery applications.
34190400	1	78	theme	surgery	293:299	arg1	treatment					245:253	treatment	245:253	treatment of periodontal disease and postdental surgery disorders	245:309	Mucoadhesive buccal patch is a promising dosage form for a successful oral drug delivery, which provides unique advantages for various applications such as treatment of periodontal disease and postdental surgery disorders.
34190400	5	79	theme	scanning	976:983	arg1	FESEM					1006:1010	FESEM	1006:1010	FESEM	1006:1010	Buccal patches were characterized using scanning electron microscopy (SEM) and field emission scanning electron microscopy (FESEM) and also evaluated in terms of physicomechanical parameters such as pH, weight, thickness, tensile strength, folding endurance, and mucoadhesive properties.
34190400	5	79	theme	scanning	976:983	arg1	microscopy					994:1003	scanning electron microscopy	976:1003	scanning electron microscopy (FESEM)	976:1011	Buccal patches were characterized using scanning electron microscopy (SEM) and field emission scanning electron microscopy (FESEM) and also evaluated in terms of physicomechanical parameters such as pH, weight, thickness, tensile strength, folding endurance, and mucoadhesive properties.
34190400	2	80	theme	therapeutic	438:448	arg1	applications					450:461	therapeutic applications	438:461	therapeutic applications	438:461	The aim of this study is to synthesize a novel multifunctional mucoadhesive buccal patch in a multilayer reservoir design for therapeutic applications.
34190400	0	81	theme	patch	51:55	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication of multifunctional mucoadhesive buccal patch for drug delivery applications.	0:87	Fabrication of multifunctional mucoadhesive buccal patch for drug delivery applications.
34190400	7	82	theme	electrospinning	1295:1309	arg1	solution					1311:1318	the electrospinning solution	1291:1318	the electrospinning solution	1291:1318	The effect of genipin addition to the electrospinning solution was also studied on mucoadhesive and swelling properties.
34190400	8	83	theme	patches	1407:1413	arg1	viability					1387:1395	The cell viability	1378:1395	The cell viability of buccal patches	1378:1413	The cell viability of buccal patches was assessed by methylthiazolydiphenyl-tetrazolium bromide test on L929 fibroblast cell line.
34190400	2	84	theme	multilayer	406:415	arg1	design					427:432	a multilayer reservoir design	404:432	a multilayer reservoir design	404:432	The aim of this study is to synthesize a novel multifunctional mucoadhesive buccal patch in a multilayer reservoir design for therapeutic applications.
34190400	0	85	theme	drug	61:64	arg1	applications					75:86	drug delivery applications	61:86	drug delivery applications	61:86	Fabrication of multifunctional mucoadhesive buccal patch for drug delivery applications.
34190400	10	86	with	patch	1779:1783	arg1	design					1814:1819	design	1814:1819	design	1814:1819	The synthesized multifunctional mucoadhesive patch with a novel composition and design has a great potential for oral therapeutic applications.
34190400	10	86	with	patch	1779:1783	arg1	composition					1798:1808	a novel composition	1790:1808	a novel composition	1790:1808	The synthesized multifunctional mucoadhesive patch with a novel composition and design has a great potential for oral therapeutic applications.
34190400	9	87	theme	ideal	1633:1637	arg1	activity					1650:1657	an ideal hemostatic activity	1630:1657	an ideal hemostatic activity	1630:1657	The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria.
34190400	4	88	theme	tongue	851:856	arg1	movement					858:865	tongue movement	851:865	tongue movement	851:865	An electrospun poly(caprolactone) (PCL) was used as an impermeable backing layer to protect the mucoadhesive component from tongue movement and drug loss.
34190400	3	89	theme	chitosan/poly	532:544	arg1	/ibuprofen					565:574	chitosan/poly(vinylalcohol) (PVA)/ibuprofen	532:574	chitosan/poly(vinylalcohol) (PVA)/ibuprofen	532:574	The patches were fabricated through simultaneous electrospinning of chitosan/poly(vinylalcohol) (PVA)/ibuprofen and electrospraying of phenylalanine amino acid nanotubes (PhNTs) containing metronidazole into the electrospun mats through a layer-by-layer process.
34190400	7	90	theme	genipin	1271:1277	arg1	addition					1279:1286	genipin addition	1271:1286	genipin addition	1271:1286	The effect of genipin addition to the electrospinning solution was also studied on mucoadhesive and swelling properties.
34190400	3	91	contain	containing	642:651	arg1	PhNTs					635:639	PhNTs	635:639	PhNTs	635:639	The patches were fabricated through simultaneous electrospinning of chitosan/poly(vinylalcohol) (PVA)/ibuprofen and electrospraying of phenylalanine amino acid nanotubes (PhNTs) containing metronidazole into the electrospun mats through a layer-by-layer process.
34190400	3	91	contain	containing	642:651	arg2	metronidazole					653:665	metronidazole	653:665	metronidazole into the electrospun mats	653:691	The patches were fabricated through simultaneous electrospinning of chitosan/poly(vinylalcohol) (PVA)/ibuprofen and electrospraying of phenylalanine amino acid nanotubes (PhNTs) containing metronidazole into the electrospun mats through a layer-by-layer process.
34190400	3	91	contain	containing	642:651	arg1	nanotubes					624:632	phenylalanine amino acid nanotubes	599:632	phenylalanine amino acid nanotubes (PhNTs) containing metronidazole into the electrospun mats through a layer-by-layer process	599:724	The patches were fabricated through simultaneous electrospinning of chitosan/poly(vinylalcohol) (PVA)/ibuprofen and electrospraying of phenylalanine amino acid nanotubes (PhNTs) containing metronidazole into the electrospun mats through a layer-by-layer process.
34190400	9	92	theme	crosslinking	1592:1603	arg1	0.05 g					1612:1617	0.05 g	1612:1617	0.05 g	1612:1617	The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria.
34190400	9	92	theme	crosslinking	1592:1603	arg1	agent					1605:1609	crosslinking agent	1592:1609	crosslinking agent (0.05 g)	1592:1618	The patch with an optimal amount of mucoadhesive polymers (PVA/chitosan 80:20) and crosslinking agent (0.05 g) indicated an ideal hemostatic activity along with antibacterial properties against Streptococcus mutans bacteria.
34190400	8	93	theme	methylthiazolydiphenyl-tetrazolium	1431:1464	arg1	test					1474:1477	methylthiazolydiphenyl-tetrazolium bromide test	1431:1477	methylthiazolydiphenyl-tetrazolium bromide test on L929 fibroblast cell line	1431:1506	The cell viability of buccal patches was assessed by methylthiazolydiphenyl-tetrazolium bromide test on L929 fibroblast cell line.
34190400	2	94	theme	study	328:332	arg1	aim					316:318	The aim	312:318	The aim of this study	312:332	The aim of this study is to synthesize a novel multifunctional mucoadhesive buccal patch in a multilayer reservoir design for therapeutic applications.
34190400	2	95	theme	multifunctional	359:373	arg1	patch					395:399	a novel multifunctional mucoadhesive buccal patch	351:399	a novel multifunctional mucoadhesive buccal patch in a multilayer reservoir design for therapeutic applications	351:461	The aim of this study is to synthesize a novel multifunctional mucoadhesive buccal patch in a multilayer reservoir design for therapeutic applications.
34190400	4	96	used	used	771:774	arg2	layer					802:806	an impermeable backing layer	779:806	an impermeable backing layer	779:806	An electrospun poly(caprolactone) (PCL) was used as an impermeable backing layer to protect the mucoadhesive component from tongue movement and drug loss.
34190400	4	96	used	used	771:774	arg2	electrospun					730:740	An electrospun poly(caprolactone) (PCL)	727:765	An electrospun poly(caprolactone) (PCL)	727:765	An electrospun poly(caprolactone) (PCL) was used as an impermeable backing layer to protect the mucoadhesive component from tongue movement and drug loss.
34555401	7	0	from	effect	1434:1439	arg1	matrix					1467:1472	PVA matrix	1463:1472	PVA matrix	1463:1472	Thus, the combined effect of CNC, rGO and Ag in PVA matrix distinctively resulted into a multifunctional hybrid nanocomposite for potential use in tissue engineering and packaging applications.
34555401	4	1	theme	Coupled	836:842	arg1	ICP-MS					870:875	ICP-MS	870:875	ICP-MS	870:875	PVA/CNC/rGO/Ag nanocomposite showed the Ag+ ions sustained release from PVA studied using Inductively Coupled Plasma Mass Spectroscopy (ICP-MS).
34555401	4	1	theme	Coupled	836:842	arg1	Spectroscopy					856:867	Inductively Coupled Plasma Mass Spectroscopy	824:867	Inductively Coupled Plasma Mass Spectroscopy (ICP-MS)	824:876	PVA/CNC/rGO/Ag nanocomposite showed the Ag+ ions sustained release from PVA studied using Inductively Coupled Plasma Mass Spectroscopy (ICP-MS).
34555401	2	2	theme	reduction	365:373	arg1	process					375:381	One-step reduction process	356:381	One-step reduction process	356:381	One-step reduction process was followed, composed of reducing graphene oxide (GO) and silver nitrate (AgNO3) into rGO and Ag nanoparticles through hydrazine hydrate (chemical reduction method), respectively.
34555401	4	3	theme	Mass	851:854	arg1	ICP-MS					870:875	ICP-MS	870:875	ICP-MS	870:875	PVA/CNC/rGO/Ag nanocomposite showed the Ag+ ions sustained release from PVA studied using Inductively Coupled Plasma Mass Spectroscopy (ICP-MS).
34555401	4	3	theme	Mass	851:854	arg1	Spectroscopy					856:867	Inductively Coupled Plasma Mass Spectroscopy	824:867	Inductively Coupled Plasma Mass Spectroscopy (ICP-MS)	824:876	PVA/CNC/rGO/Ag nanocomposite showed the Ag+ ions sustained release from PVA studied using Inductively Coupled Plasma Mass Spectroscopy (ICP-MS).
34555401	6	4	theme	cell	1225:1228	arg1	line					1230:1233	HEK-293 cell line	1217:1233	HEK-293 cell line	1217:1233	All prepared nanocomposites with different wt% of Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag) were non-toxic to HEK-293 cell line and exhibited improved antibacterial property against E. coli and S. aureus due to a combination of Ag+ ions (release from Ag nanoparticles) and rGO (having antibacterial effect).
34555401	6	5	theme	wt	1158:1159	arg1	Ag					1165:1166	Ag	1165:1166	Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag)	1165:1197	All prepared nanocomposites with different wt% of Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag) were non-toxic to HEK-293 cell line and exhibited improved antibacterial property against E. coli and S. aureus due to a combination of Ag+ ions (release from Ag nanoparticles) and rGO (having antibacterial effect).
34555401	6	5	theme	wt	1158:1159	arg1	%					1160:1160	different wt%	1148:1160	different wt% of Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag)	1148:1197	All prepared nanocomposites with different wt% of Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag) were non-toxic to HEK-293 cell line and exhibited improved antibacterial property against E. coli and S. aureus due to a combination of Ag+ ions (release from Ag nanoparticles) and rGO (having antibacterial effect).
34555401	5	6	dep	FTIR	1000:1003	arg1	Transform					1014:1022	Transform	1014:1022	Transform Infrared Spectroscopy	1014:1044	The incorporation and elemental composition of CNC, rGO and Ag nanoparticles into nanocomposite were interpreted through FTIR (Fourier Transform Infrared Spectroscopy) and XPS (X-ray photoelectron spectroscopy) technique, respectively.
34555401	3	7	theme	PVA	670:672	arg1	%					665:665	184%	662:665	184% of PVA	662:672	Uniformly dispersed CNC, rGO and Ag nanoparticles in PVA matrix led to an increment in modulus by 184% of PVA demonstrating the reinforcement outcome of CNC, rGO and Ag.
34555401	3	7	theme	PVA	670:672	arg1	PVA					670:672	PVA	670:672	PVA	670:672	Uniformly dispersed CNC, rGO and Ag nanoparticles in PVA matrix led to an increment in modulus by 184% of PVA demonstrating the reinforcement outcome of CNC, rGO and Ag.
34555401	4	8	dep	showed	763:768	arg1	sustained					783:791	sustained	783:791	showed the Ag+ ions sustained release from PVA studied using Inductively Coupled Plasma Mass Spectroscopy (ICP-MS)	763:876	PVA/CNC/rGO/Ag nanocomposite showed the Ag+ ions sustained release from PVA studied using Inductively Coupled Plasma Mass Spectroscopy (ICP-MS).
34555401	3	9	theme	CNC	717:719	arg1	outcome					706:712	the reinforcement outcome	688:712	the reinforcement outcome of CNC, rGO and Ag	688:731	Uniformly dispersed CNC, rGO and Ag nanoparticles in PVA matrix led to an increment in modulus by 184% of PVA demonstrating the reinforcement outcome of CNC, rGO and Ag.
34555401	7	10	theme	tissue	1562:1567	arg1	engineering					1569:1579	tissue engineering	1562:1579	tissue engineering	1562:1579	Thus, the combined effect of CNC, rGO and Ag in PVA matrix distinctively resulted into a multifunctional hybrid nanocomposite for potential use in tissue engineering and packaging applications.
34555401	6	11	theme	prepared	1119:1126	arg1	nanocomposites					1128:1141	All prepared nanocomposites	1115:1141	All prepared nanocomposites with different wt% of Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag)	1115:1197	All prepared nanocomposites with different wt% of Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag) were non-toxic to HEK-293 cell line and exhibited improved antibacterial property against E. coli and S. aureus due to a combination of Ag+ ions (release from Ag nanoparticles) and rGO (having antibacterial effect).
34555401	1	12	theme	graphene	244:251	arg1	rGO					260:262	rGO	260:262	rGO	260:262	Towards fabricating a hybrid biodegradable multifunctional nanocomposite, cellulose nanocrystal (CNC), reduced graphene oxide (rGO) and silver (Ag) nanoparticles were reinforced into polyvinyl alcohol (PVA) polymer matrix.
34555401	1	12	theme	graphene	244:251	arg1	oxide					253:257	reduced graphene oxide	236:257	reduced graphene oxide (rGO)	236:263	Towards fabricating a hybrid biodegradable multifunctional nanocomposite, cellulose nanocrystal (CNC), reduced graphene oxide (rGO) and silver (Ag) nanoparticles were reinforced into polyvinyl alcohol (PVA) polymer matrix.
34555401	2	13	theme	reduction	531:539	arg1	method					541:546	chemical reduction method	522:546	chemical reduction method	522:546	One-step reduction process was followed, composed of reducing graphene oxide (GO) and silver nitrate (AgNO3) into rGO and Ag nanoparticles through hydrazine hydrate (chemical reduction method), respectively.
34555401	2	13	theme	reduction	531:539	arg1	hydrate					513:519	hydrazine hydrate	503:519	hydrazine hydrate (chemical reduction method)	503:547	One-step reduction process was followed, composed of reducing graphene oxide (GO) and silver nitrate (AgNO3) into rGO and Ag nanoparticles through hydrazine hydrate (chemical reduction method), respectively.
34555401	3	14	theme	Ag	730:731	arg1	outcome					706:712	the reinforcement outcome	688:712	the reinforcement outcome of CNC, rGO and Ag	688:731	Uniformly dispersed CNC, rGO and Ag nanoparticles in PVA matrix led to an increment in modulus by 184% of PVA demonstrating the reinforcement outcome of CNC, rGO and Ag.
34555401	5	15	theme	XPS	1051:1053	arg1	spectroscopy					1076:1087	X-ray photoelectron spectroscopy	1056:1087	X-ray photoelectron spectroscopy	1056:1087	The incorporation and elemental composition of CNC, rGO and Ag nanoparticles into nanocomposite were interpreted through FTIR (Fourier Transform Infrared Spectroscopy) and XPS (X-ray photoelectron spectroscopy) technique, respectively.
34555401	5	15	theme	XPS	1051:1053	arg1	technique					1090:1098	FTIR (Fourier Transform Infrared Spectroscopy) and XPS (X-ray photoelectron spectroscopy) technique	1000:1098	technique	1090:1098	The incorporation and elemental composition of CNC, rGO and Ag nanoparticles into nanocomposite were interpreted through FTIR (Fourier Transform Infrared Spectroscopy) and XPS (X-ray photoelectron spectroscopy) technique, respectively.
34555401	3	16	theme	rGO	589:591	arg1	nanoparticles					600:612	Uniformly dispersed CNC, rGO and Ag nanoparticles	564:612	Uniformly dispersed CNC, rGO and Ag nanoparticles in PVA matrix	564:626	Uniformly dispersed CNC, rGO and Ag nanoparticles in PVA matrix led to an increment in modulus by 184% of PVA demonstrating the reinforcement outcome of CNC, rGO and Ag.
34555401	6	17	with	nanocomposites	1128:1141	arg1	Ag					1165:1166	Ag	1165:1166	Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag)	1165:1197	All prepared nanocomposites with different wt% of Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag) were non-toxic to HEK-293 cell line and exhibited improved antibacterial property against E. coli and S. aureus due to a combination of Ag+ ions (release from Ag nanoparticles) and rGO (having antibacterial effect).
34555401	6	17	with	nanocomposites	1128:1141	arg1	%					1160:1160	different wt%	1148:1160	different wt% of Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag)	1148:1197	All prepared nanocomposites with different wt% of Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag) were non-toxic to HEK-293 cell line and exhibited improved antibacterial property against E. coli and S. aureus due to a combination of Ag+ ions (release from Ag nanoparticles) and rGO (having antibacterial effect).
34555401	3	18	theme	rGO	722:724	arg1	outcome					706:712	the reinforcement outcome	688:712	the reinforcement outcome of CNC, rGO and Ag	688:731	Uniformly dispersed CNC, rGO and Ag nanoparticles in PVA matrix led to an increment in modulus by 184% of PVA demonstrating the reinforcement outcome of CNC, rGO and Ag.
34555401	3	19	theme	CNC	584:586	arg1	nanoparticles					600:612	Uniformly dispersed CNC, rGO and Ag nanoparticles	564:612	Uniformly dispersed CNC, rGO and Ag nanoparticles in PVA matrix	564:626	Uniformly dispersed CNC, rGO and Ag nanoparticles in PVA matrix led to an increment in modulus by 184% of PVA demonstrating the reinforcement outcome of CNC, rGO and Ag.
34555401	1	20	theme	hybrid	155:160	arg1	nanocomposite					192:204	a hybrid biodegradable multifunctional nanocomposite	153:204	a hybrid biodegradable multifunctional nanocomposite	153:204	Towards fabricating a hybrid biodegradable multifunctional nanocomposite, cellulose nanocrystal (CNC), reduced graphene oxide (rGO) and silver (Ag) nanoparticles were reinforced into polyvinyl alcohol (PVA) polymer matrix.
34555401	7	21	theme	hybrid	1520:1525	arg1	nanocomposite					1527:1539	a multifunctional hybrid nanocomposite	1502:1539	a multifunctional hybrid nanocomposite for potential use in tissue engineering and packaging applications	1502:1606	Thus, the combined effect of CNC, rGO and Ag in PVA matrix distinctively resulted into a multifunctional hybrid nanocomposite for potential use in tissue engineering and packaging applications.
34555401	6	22	theme	improved	1249:1256	arg1	property					1272:1279	improved antibacterial property	1249:1279	improved antibacterial property against E. coli and S. aureus	1249:1309	All prepared nanocomposites with different wt% of Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag) were non-toxic to HEK-293 cell line and exhibited improved antibacterial property against E. coli and S. aureus due to a combination of Ag+ ions (release from Ag nanoparticles) and rGO (having antibacterial effect).
34555401	5	23	theme	Infrared	1024:1031	arg1	Spectroscopy					1033:1044	Infrared Spectroscopy	1024:1044	Infrared Spectroscopy	1024:1044	The incorporation and elemental composition of CNC, rGO and Ag nanoparticles into nanocomposite were interpreted through FTIR (Fourier Transform Infrared Spectroscopy) and XPS (X-ray photoelectron spectroscopy) technique, respectively.
34555401	1	24	theme	multifunctional	176:190	arg1	nanocomposite					192:204	a hybrid biodegradable multifunctional nanocomposite	153:204	a hybrid biodegradable multifunctional nanocomposite	153:204	Towards fabricating a hybrid biodegradable multifunctional nanocomposite, cellulose nanocrystal (CNC), reduced graphene oxide (rGO) and silver (Ag) nanoparticles were reinforced into polyvinyl alcohol (PVA) polymer matrix.
34555401	6	25	theme	Ag+	1335:1337	arg1	ions					1339:1342	Ag+ ions	1335:1342	Ag+ ions (release from Ag nanoparticles)	1335:1374	All prepared nanocomposites with different wt% of Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag) were non-toxic to HEK-293 cell line and exhibited improved antibacterial property against E. coli and S. aureus due to a combination of Ag+ ions (release from Ag nanoparticles) and rGO (having antibacterial effect).
34555401	1	26	theme	polymer	340:346	arg1	matrix					348:353	polyvinyl alcohol (PVA) polymer matrix	316:353	polyvinyl alcohol (PVA) polymer matrix	316:353	Towards fabricating a hybrid biodegradable multifunctional nanocomposite, cellulose nanocrystal (CNC), reduced graphene oxide (rGO) and silver (Ag) nanoparticles were reinforced into polyvinyl alcohol (PVA) polymer matrix.
34555401	3	27	theme	Ag	597:598	arg1	nanoparticles					600:612	Uniformly dispersed CNC, rGO and Ag nanoparticles	564:612	Uniformly dispersed CNC, rGO and Ag nanoparticles in PVA matrix	564:626	Uniformly dispersed CNC, rGO and Ag nanoparticles in PVA matrix led to an increment in modulus by 184% of PVA demonstrating the reinforcement outcome of CNC, rGO and Ag.
34555401	1	28	theme	nanocrystal	217:227	arg1	nanoparticles					281:293	cellulose nanocrystal (CNC), reduced graphene oxide (rGO) and silver (Ag) nanoparticles	207:293	cellulose nanocrystal (CNC), reduced graphene oxide (rGO) and silver (Ag) nanoparticles	207:293	Towards fabricating a hybrid biodegradable multifunctional nanocomposite, cellulose nanocrystal (CNC), reduced graphene oxide (rGO) and silver (Ag) nanoparticles were reinforced into polyvinyl alcohol (PVA) polymer matrix.
34555401	5	29	dep	incorporation	883:895	arg1	The					879:881	The	879:881	The	879:881	The incorporation and elemental composition of CNC, rGO and Ag nanoparticles into nanocomposite were interpreted through FTIR (Fourier Transform Infrared Spectroscopy) and XPS (X-ray photoelectron spectroscopy) technique, respectively.
34555401	5	30	theme	X-ray	1056:1060	arg1	spectroscopy					1076:1087	X-ray photoelectron spectroscopy	1056:1087	X-ray photoelectron spectroscopy	1056:1087	The incorporation and elemental composition of CNC, rGO and Ag nanoparticles into nanocomposite were interpreted through FTIR (Fourier Transform Infrared Spectroscopy) and XPS (X-ray photoelectron spectroscopy) technique, respectively.
34555401	5	30	theme	X-ray	1056:1060	arg1	technique					1090:1098	FTIR (Fourier Transform Infrared Spectroscopy) and XPS (X-ray photoelectron spectroscopy) technique	1000:1098	technique	1090:1098	The incorporation and elemental composition of CNC, rGO and Ag nanoparticles into nanocomposite were interpreted through FTIR (Fourier Transform Infrared Spectroscopy) and XPS (X-ray photoelectron spectroscopy) technique, respectively.
34555401	5	31	theme	rGO	931:933	arg1	nanoparticles					942:954	CNC, rGO and Ag nanoparticles	926:954	CNC, rGO and Ag nanoparticles	926:954	The incorporation and elemental composition of CNC, rGO and Ag nanoparticles into nanocomposite were interpreted through FTIR (Fourier Transform Infrared Spectroscopy) and XPS (X-ray photoelectron spectroscopy) technique, respectively.
34555401	2	32	theme	hydrazine	503:511	arg1	method					541:546	chemical reduction method	522:546	chemical reduction method	522:546	One-step reduction process was followed, composed of reducing graphene oxide (GO) and silver nitrate (AgNO3) into rGO and Ag nanoparticles through hydrazine hydrate (chemical reduction method), respectively.
34555401	2	32	theme	hydrazine	503:511	arg1	hydrate					513:519	hydrazine hydrate	503:519	hydrazine hydrate (chemical reduction method)	503:547	One-step reduction process was followed, composed of reducing graphene oxide (GO) and silver nitrate (AgNO3) into rGO and Ag nanoparticles through hydrazine hydrate (chemical reduction method), respectively.
34555401	2	33	dep	followed	387:394	arg1	composed					397:404	composed	397:404	composed of reducing graphene oxide (GO) and silver nitrate (AgNO3) into rGO and Ag nanoparticles through hydrazine hydrate (chemical reduction method), respectively	397:561	One-step reduction process was followed, composed of reducing graphene oxide (GO) and silver nitrate (AgNO3) into rGO and Ag nanoparticles through hydrazine hydrate (chemical reduction method), respectively.
34555401	4	34	from	PVA	806:808	arg1	release					793:799	release	793:799	release from PVA studied using Inductively Coupled Plasma Mass Spectroscopy (ICP-MS)	793:876	PVA/CNC/rGO/Ag nanocomposite showed the Ag+ ions sustained release from PVA studied using Inductively Coupled Plasma Mass Spectroscopy (ICP-MS).
34555401	7	35	from	use	1555:1557	arg1	packaging					1585:1593	packaging	1585:1593	packaging	1585:1593	Thus, the combined effect of CNC, rGO and Ag in PVA matrix distinctively resulted into a multifunctional hybrid nanocomposite for potential use in tissue engineering and packaging applications.
34555401	7	35	from	use	1555:1557	arg1	engineering					1569:1579	tissue engineering	1562:1579	tissue engineering	1562:1579	Thus, the combined effect of CNC, rGO and Ag in PVA matrix distinctively resulted into a multifunctional hybrid nanocomposite for potential use in tissue engineering and packaging applications.
34555401	5	36	theme	Ag	939:940	arg1	nanoparticles					942:954	CNC, rGO and Ag nanoparticles	926:954	CNC, rGO and Ag nanoparticles	926:954	The incorporation and elemental composition of CNC, rGO and Ag nanoparticles into nanocomposite were interpreted through FTIR (Fourier Transform Infrared Spectroscopy) and XPS (X-ray photoelectron spectroscopy) technique, respectively.
34555401	0	37	theme	Cellulose	0:8	arg1	oxide					61:65	Cellulose nanocrystals‑silver nanoparticles-reduced graphene oxide	0:65	Cellulose nanocrystals‑silver nanoparticles-reduced graphene oxide	0:65	Cellulose nanocrystals‑silver nanoparticles-reduced graphene oxide based hybrid PVA nanocomposites and its antimicrobial properties.
34555401	0	38	theme	nanoparticles-reduced	30:50	arg1	oxide					61:65	Cellulose nanocrystals‑silver nanoparticles-reduced graphene oxide	0:65	Cellulose nanocrystals‑silver nanoparticles-reduced graphene oxide	0:65	Cellulose nanocrystals‑silver nanoparticles-reduced graphene oxide based hybrid PVA nanocomposites and its antimicrobial properties.
34555401	7	39	theme	rGO	1449:1451	arg1	effect					1434:1439	the combined effect	1421:1439	the combined effect of CNC, rGO and Ag in PVA matrix	1421:1472	Thus, the combined effect of CNC, rGO and Ag in PVA matrix distinctively resulted into a multifunctional hybrid nanocomposite for potential use in tissue engineering and packaging applications.
34555401	5	40	theme	CNC	926:928	arg1	nanoparticles					942:954	CNC, rGO and Ag nanoparticles	926:954	CNC, rGO and Ag nanoparticles	926:954	The incorporation and elemental composition of CNC, rGO and Ag nanoparticles into nanocomposite were interpreted through FTIR (Fourier Transform Infrared Spectroscopy) and XPS (X-ray photoelectron spectroscopy) technique, respectively.
34555401	2	41	dep	oxide	427:431	arg1	GO					434:435	GO	434:435	GO	434:435	One-step reduction process was followed, composed of reducing graphene oxide (GO) and silver nitrate (AgNO3) into rGO and Ag nanoparticles through hydrazine hydrate (chemical reduction method), respectively.
34555401	1	42	theme	alcohol	326:332	arg1	matrix					348:353	polyvinyl alcohol (PVA) polymer matrix	316:353	polyvinyl alcohol (PVA) polymer matrix	316:353	Towards fabricating a hybrid biodegradable multifunctional nanocomposite, cellulose nanocrystal (CNC), reduced graphene oxide (rGO) and silver (Ag) nanoparticles were reinforced into polyvinyl alcohol (PVA) polymer matrix.
34555401	7	43	theme	CNC	1444:1446	arg1	effect					1434:1439	the combined effect	1421:1439	the combined effect of CNC, rGO and Ag in PVA matrix	1421:1472	Thus, the combined effect of CNC, rGO and Ag in PVA matrix distinctively resulted into a multifunctional hybrid nanocomposite for potential use in tissue engineering and packaging applications.
34555401	2	44	theme	One-step	356:363	arg1	process					375:381	One-step reduction process	356:381	One-step reduction process	356:381	One-step reduction process was followed, composed of reducing graphene oxide (GO) and silver nitrate (AgNO3) into rGO and Ag nanoparticles through hydrazine hydrate (chemical reduction method), respectively.
34555401	0	45	theme	hybrid	73:78	arg1	nanocomposites					84:97	hybrid PVA nanocomposites	73:97	hybrid PVA nanocomposites	73:97	Cellulose nanocrystals‑silver nanoparticles-reduced graphene oxide based hybrid PVA nanocomposites and its antimicrobial properties.
34555401	6	46	theme	antibacterial	1392:1404	arg1	effect					1406:1411	antibacterial effect	1392:1411	antibacterial effect	1392:1411	All prepared nanocomposites with different wt% of Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag) were non-toxic to HEK-293 cell line and exhibited improved antibacterial property against E. coli and S. aureus due to a combination of Ag+ ions (release from Ag nanoparticles) and rGO (having antibacterial effect).
34555401	4	47	theme	Plasma	844:849	arg1	ICP-MS					870:875	ICP-MS	870:875	ICP-MS	870:875	PVA/CNC/rGO/Ag nanocomposite showed the Ag+ ions sustained release from PVA studied using Inductively Coupled Plasma Mass Spectroscopy (ICP-MS).
34555401	4	47	theme	Plasma	844:849	arg1	Spectroscopy					856:867	Inductively Coupled Plasma Mass Spectroscopy	824:867	Inductively Coupled Plasma Mass Spectroscopy (ICP-MS)	824:876	PVA/CNC/rGO/Ag nanocomposite showed the Ag+ ions sustained release from PVA studied using Inductively Coupled Plasma Mass Spectroscopy (ICP-MS).
34555401	3	48	theme	reinforcement	692:704	arg1	outcome					706:712	the reinforcement outcome	688:712	the reinforcement outcome of CNC, rGO and Ag	688:731	Uniformly dispersed CNC, rGO and Ag nanoparticles in PVA matrix led to an increment in modulus by 184% of PVA demonstrating the reinforcement outcome of CNC, rGO and Ag.
34555401	6	49	theme	HEK-293	1217:1223	arg1	line					1230:1233	HEK-293 cell line	1217:1233	HEK-293 cell line	1217:1233	All prepared nanocomposites with different wt% of Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag) were non-toxic to HEK-293 cell line and exhibited improved antibacterial property against E. coli and S. aureus due to a combination of Ag+ ions (release from Ag nanoparticles) and rGO (having antibacterial effect).
34555401	6	50	theme	different	1148:1156	arg1	Ag					1165:1166	Ag	1165:1166	Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag)	1165:1197	All prepared nanocomposites with different wt% of Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag) were non-toxic to HEK-293 cell line and exhibited improved antibacterial property against E. coli and S. aureus due to a combination of Ag+ ions (release from Ag nanoparticles) and rGO (having antibacterial effect).
34555401	6	50	theme	different	1148:1156	arg1	%					1160:1160	different wt%	1148:1160	different wt% of Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag)	1148:1197	All prepared nanocomposites with different wt% of Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag) were non-toxic to HEK-293 cell line and exhibited improved antibacterial property against E. coli and S. aureus due to a combination of Ag+ ions (release from Ag nanoparticles) and rGO (having antibacterial effect).
34555401	1	51	theme	reduced	236:242	arg1	rGO					260:262	rGO	260:262	rGO	260:262	Towards fabricating a hybrid biodegradable multifunctional nanocomposite, cellulose nanocrystal (CNC), reduced graphene oxide (rGO) and silver (Ag) nanoparticles were reinforced into polyvinyl alcohol (PVA) polymer matrix.
34555401	1	51	theme	reduced	236:242	arg1	oxide					253:257	reduced graphene oxide	236:257	reduced graphene oxide (rGO)	236:263	Towards fabricating a hybrid biodegradable multifunctional nanocomposite, cellulose nanocrystal (CNC), reduced graphene oxide (rGO) and silver (Ag) nanoparticles were reinforced into polyvinyl alcohol (PVA) polymer matrix.
34555401	7	52	theme	potential	1545:1553	arg1	use					1555:1557	potential use	1545:1557	potential use in tissue engineering and packaging applications	1545:1606	Thus, the combined effect of CNC, rGO and Ag in PVA matrix distinctively resulted into a multifunctional hybrid nanocomposite for potential use in tissue engineering and packaging applications.
34555401	6	53	from	nanoparticles	1361:1373	arg1	release					1345:1351	release	1345:1351	release from Ag nanoparticles	1345:1373	All prepared nanocomposites with different wt% of Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag) were non-toxic to HEK-293 cell line and exhibited improved antibacterial property against E. coli and S. aureus due to a combination of Ag+ ions (release from Ag nanoparticles) and rGO (having antibacterial effect).
34555401	1	54	theme	oxide	253:257	arg1	nanoparticles					281:293	cellulose nanocrystal (CNC), reduced graphene oxide (rGO) and silver (Ag) nanoparticles	207:293	cellulose nanocrystal (CNC), reduced graphene oxide (rGO) and silver (Ag) nanoparticles	207:293	Towards fabricating a hybrid biodegradable multifunctional nanocomposite, cellulose nanocrystal (CNC), reduced graphene oxide (rGO) and silver (Ag) nanoparticles were reinforced into polyvinyl alcohol (PVA) polymer matrix.
34555401	2	55	theme	chemical	522:529	arg1	method					541:546	chemical reduction method	522:546	chemical reduction method	522:546	One-step reduction process was followed, composed of reducing graphene oxide (GO) and silver nitrate (AgNO3) into rGO and Ag nanoparticles through hydrazine hydrate (chemical reduction method), respectively.
34555401	2	55	theme	chemical	522:529	arg1	hydrate					513:519	hydrazine hydrate	503:519	hydrazine hydrate (chemical reduction method)	503:547	One-step reduction process was followed, composed of reducing graphene oxide (GO) and silver nitrate (AgNO3) into rGO and Ag nanoparticles through hydrazine hydrate (chemical reduction method), respectively.
34555401	3	56	theme	dispersed	574:582	arg1	nanoparticles					600:612	Uniformly dispersed CNC, rGO and Ag nanoparticles	564:612	Uniformly dispersed CNC, rGO and Ag nanoparticles in PVA matrix	564:626	Uniformly dispersed CNC, rGO and Ag nanoparticles in PVA matrix led to an increment in modulus by 184% of PVA demonstrating the reinforcement outcome of CNC, rGO and Ag.
34555401	7	57	theme	multifunctional	1504:1518	arg1	nanocomposite					1527:1539	a multifunctional hybrid nanocomposite	1502:1539	a multifunctional hybrid nanocomposite for potential use in tissue engineering and packaging applications	1502:1606	Thus, the combined effect of CNC, rGO and Ag in PVA matrix distinctively resulted into a multifunctional hybrid nanocomposite for potential use in tissue engineering and packaging applications.
34555401	6	58	dep	Ag	1165:1166	arg1	PVA					1169:1171	PVA	1169:1171	PVA	1169:1171	All prepared nanocomposites with different wt% of Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag) were non-toxic to HEK-293 cell line and exhibited improved antibacterial property against E. coli and S. aureus due to a combination of Ag+ ions (release from Ag nanoparticles) and rGO (having antibacterial effect).
34555401	6	58	dep	Ag	1165:1166	arg1	PVA/CNC/rGO/Ag					1183:1196	PVA/CNC/rGO/Ag	1183:1196	PVA/CNC/rGO/Ag	1183:1196	All prepared nanocomposites with different wt% of Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag) were non-toxic to HEK-293 cell line and exhibited improved antibacterial property against E. coli and S. aureus due to a combination of Ag+ ions (release from Ag nanoparticles) and rGO (having antibacterial effect).
34555401	6	59	theme	antibacterial	1258:1270	arg1	property					1272:1279	improved antibacterial property	1249:1279	improved antibacterial property against E. coli and S. aureus	1249:1309	All prepared nanocomposites with different wt% of Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag) were non-toxic to HEK-293 cell line and exhibited improved antibacterial property against E. coli and S. aureus due to a combination of Ag+ ions (release from Ag nanoparticles) and rGO (having antibacterial effect).
34555401	1	60	theme	biodegradable	162:174	arg1	nanocomposite					192:204	a hybrid biodegradable multifunctional nanocomposite	153:204	a hybrid biodegradable multifunctional nanocomposite	153:204	Towards fabricating a hybrid biodegradable multifunctional nanocomposite, cellulose nanocrystal (CNC), reduced graphene oxide (rGO) and silver (Ag) nanoparticles were reinforced into polyvinyl alcohol (PVA) polymer matrix.
34555401	7	61	dep	engineering	1569:1579	arg1	applications					1595:1606	applications	1595:1606	applications	1595:1606	Thus, the combined effect of CNC, rGO and Ag in PVA matrix distinctively resulted into a multifunctional hybrid nanocomposite for potential use in tissue engineering and packaging applications.
34555401	6	62	theme	ions	1339:1342	arg1	combination					1320:1330	a combination	1318:1330	a combination of Ag+ ions (release from Ag nanoparticles) and rGO (having antibacterial effect)	1318:1412	All prepared nanocomposites with different wt% of Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag) were non-toxic to HEK-293 cell line and exhibited improved antibacterial property against E. coli and S. aureus due to a combination of Ag+ ions (release from Ag nanoparticles) and rGO (having antibacterial effect).
34555401	2	63	theme	Ag	478:479	arg1	nanoparticles					481:493	Ag nanoparticles	478:493	Ag nanoparticles	478:493	One-step reduction process was followed, composed of reducing graphene oxide (GO) and silver nitrate (AgNO3) into rGO and Ag nanoparticles through hydrazine hydrate (chemical reduction method), respectively.
34555401	6	64	theme	Ag	1165:1166	arg1	Ag					1165:1166	Ag	1165:1166	Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag)	1165:1197	All prepared nanocomposites with different wt% of Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag) were non-toxic to HEK-293 cell line and exhibited improved antibacterial property against E. coli and S. aureus due to a combination of Ag+ ions (release from Ag nanoparticles) and rGO (having antibacterial effect).
34555401	6	64	theme	Ag	1165:1166	arg1	%					1160:1160	different wt%	1148:1160	different wt% of Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag)	1148:1197	All prepared nanocomposites with different wt% of Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag) were non-toxic to HEK-293 cell line and exhibited improved antibacterial property against E. coli and S. aureus due to a combination of Ag+ ions (release from Ag nanoparticles) and rGO (having antibacterial effect).
34555401	0	65	theme	antimicrobial	107:119	arg1	properties					121:130	its antimicrobial properties	103:130	its antimicrobial properties	103:130	Cellulose nanocrystals‑silver nanoparticles-reduced graphene oxide based hybrid PVA nanocomposites and its antimicrobial properties.
34555401	1	66	theme	cellulose	207:215	arg1	CNC					230:232	CNC	230:232	CNC	230:232	Towards fabricating a hybrid biodegradable multifunctional nanocomposite, cellulose nanocrystal (CNC), reduced graphene oxide (rGO) and silver (Ag) nanoparticles were reinforced into polyvinyl alcohol (PVA) polymer matrix.
34555401	1	66	theme	cellulose	207:215	arg1	nanocrystal					217:227	cellulose nanocrystal	207:227	cellulose nanocrystal (CNC)	207:233	Towards fabricating a hybrid biodegradable multifunctional nanocomposite, cellulose nanocrystal (CNC), reduced graphene oxide (rGO) and silver (Ag) nanoparticles were reinforced into polyvinyl alcohol (PVA) polymer matrix.
34555401	7	67	theme	PVA	1463:1465	arg1	matrix					1467:1472	PVA matrix	1463:1472	PVA matrix	1463:1472	Thus, the combined effect of CNC, rGO and Ag in PVA matrix distinctively resulted into a multifunctional hybrid nanocomposite for potential use in tissue engineering and packaging applications.
34555401	7	68	theme	Ag	1457:1458	arg1	effect					1434:1439	the combined effect	1421:1439	the combined effect of CNC, rGO and Ag in PVA matrix	1421:1472	Thus, the combined effect of CNC, rGO and Ag in PVA matrix distinctively resulted into a multifunctional hybrid nanocomposite for potential use in tissue engineering and packaging applications.
34555401	5	69	theme	photoelectron	1062:1074	arg1	spectroscopy					1076:1087	X-ray photoelectron spectroscopy	1056:1087	X-ray photoelectron spectroscopy	1056:1087	The incorporation and elemental composition of CNC, rGO and Ag nanoparticles into nanocomposite were interpreted through FTIR (Fourier Transform Infrared Spectroscopy) and XPS (X-ray photoelectron spectroscopy) technique, respectively.
34555401	5	69	theme	photoelectron	1062:1074	arg1	technique					1090:1098	FTIR (Fourier Transform Infrared Spectroscopy) and XPS (X-ray photoelectron spectroscopy) technique	1000:1098	technique	1090:1098	The incorporation and elemental composition of CNC, rGO and Ag nanoparticles into nanocomposite were interpreted through FTIR (Fourier Transform Infrared Spectroscopy) and XPS (X-ray photoelectron spectroscopy) technique, respectively.
34555401	3	70	from	nanoparticles	600:612	arg1	matrix					621:626	PVA matrix	617:626	PVA matrix	617:626	Uniformly dispersed CNC, rGO and Ag nanoparticles in PVA matrix led to an increment in modulus by 184% of PVA demonstrating the reinforcement outcome of CNC, rGO and Ag.
34555401	0	71	theme	nanocrystals‑silver	10:28	arg1	oxide					61:65	Cellulose nanocrystals‑silver nanoparticles-reduced graphene oxide	0:65	Cellulose nanocrystals‑silver nanoparticles-reduced graphene oxide	0:65	Cellulose nanocrystals‑silver nanoparticles-reduced graphene oxide based hybrid PVA nanocomposites and its antimicrobial properties.
34555401	6	72	dep	ions	1339:1342	arg1	release					1345:1351	release	1345:1351	release from Ag nanoparticles	1345:1373	All prepared nanocomposites with different wt% of Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag) were non-toxic to HEK-293 cell line and exhibited improved antibacterial property against E. coli and S. aureus due to a combination of Ag+ ions (release from Ag nanoparticles) and rGO (having antibacterial effect).
34555401	5	73	theme	elemental	901:909	arg1	composition					911:921	elemental composition	901:921	elemental composition	901:921	The incorporation and elemental composition of CNC, rGO and Ag nanoparticles into nanocomposite were interpreted through FTIR (Fourier Transform Infrared Spectroscopy) and XPS (X-ray photoelectron spectroscopy) technique, respectively.
34555401	2	74	theme	silver	442:447	arg1	AgNO3					458:462	AgNO3	458:462	AgNO3	458:462	One-step reduction process was followed, composed of reducing graphene oxide (GO) and silver nitrate (AgNO3) into rGO and Ag nanoparticles through hydrazine hydrate (chemical reduction method), respectively.
34555401	2	74	theme	silver	442:447	arg1	nitrate					449:455	silver nitrate	442:455	silver nitrate (AgNO3)	442:463	One-step reduction process was followed, composed of reducing graphene oxide (GO) and silver nitrate (AgNO3) into rGO and Ag nanoparticles through hydrazine hydrate (chemical reduction method), respectively.
34555401	0	75	theme	graphene	52:59	arg1	oxide					61:65	Cellulose nanocrystals‑silver nanoparticles-reduced graphene oxide	0:65	Cellulose nanocrystals‑silver nanoparticles-reduced graphene oxide	0:65	Cellulose nanocrystals‑silver nanoparticles-reduced graphene oxide based hybrid PVA nanocomposites and its antimicrobial properties.
34555401	6	76	theme	Ag	1358:1359	arg1	nanoparticles					1361:1373	Ag nanoparticles	1358:1373	Ag nanoparticles	1358:1373	All prepared nanocomposites with different wt% of Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag) were non-toxic to HEK-293 cell line and exhibited improved antibacterial property against E. coli and S. aureus due to a combination of Ag+ ions (release from Ag nanoparticles) and rGO (having antibacterial effect).
34555401	1	77	theme	polyvinyl	316:324	arg1	PVA					335:337	PVA	335:337	PVA	335:337	Towards fabricating a hybrid biodegradable multifunctional nanocomposite, cellulose nanocrystal (CNC), reduced graphene oxide (rGO) and silver (Ag) nanoparticles were reinforced into polyvinyl alcohol (PVA) polymer matrix.
34555401	1	77	theme	polyvinyl	316:324	arg1	alcohol					326:332	polyvinyl alcohol	316:332	polyvinyl alcohol (PVA) polymer matrix	316:353	Towards fabricating a hybrid biodegradable multifunctional nanocomposite, cellulose nanocrystal (CNC), reduced graphene oxide (rGO) and silver (Ag) nanoparticles were reinforced into polyvinyl alcohol (PVA) polymer matrix.
34555401	3	78	theme	PVA	617:619	arg1	matrix					621:626	PVA matrix	617:626	PVA matrix	617:626	Uniformly dispersed CNC, rGO and Ag nanoparticles in PVA matrix led to an increment in modulus by 184% of PVA demonstrating the reinforcement outcome of CNC, rGO and Ag.
34555401	4	79	theme	Ag+	774:776	arg1	ions					778:781	the Ag+ ions	770:781	the Ag+ ions	770:781	PVA/CNC/rGO/Ag nanocomposite showed the Ag+ ions sustained release from PVA studied using Inductively Coupled Plasma Mass Spectroscopy (ICP-MS).
34555401	2	80	theme	graphene	418:425	arg1	oxide					427:431	graphene oxide	418:431	graphene oxide (GO)	418:436	One-step reduction process was followed, composed of reducing graphene oxide (GO) and silver nitrate (AgNO3) into rGO and Ag nanoparticles through hydrazine hydrate (chemical reduction method), respectively.
34555401	0	81	theme	PVA	80:82	arg1	nanocomposites					84:97	hybrid PVA nanocomposites	73:97	hybrid PVA nanocomposites	73:97	Cellulose nanocrystals‑silver nanoparticles-reduced graphene oxide based hybrid PVA nanocomposites and its antimicrobial properties.
34555401	6	82	dep	rGO	1380:1382	arg1	having					1385:1390	having	1385:1390	having antibacterial effect	1385:1411	All prepared nanocomposites with different wt% of Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag) were non-toxic to HEK-293 cell line and exhibited improved antibacterial property against E. coli and S. aureus due to a combination of Ag+ ions (release from Ag nanoparticles) and rGO (having antibacterial effect).
34555401	5	83	theme	nanoparticles	942:954	arg1	incorporation					883:895	incorporation	883:895	incorporation	883:895	The incorporation and elemental composition of CNC, rGO and Ag nanoparticles into nanocomposite were interpreted through FTIR (Fourier Transform Infrared Spectroscopy) and XPS (X-ray photoelectron spectroscopy) technique, respectively.
34555401	5	83	theme	nanoparticles	942:954	arg1	composition					911:921	elemental composition	901:921	elemental composition	901:921	The incorporation and elemental composition of CNC, rGO and Ag nanoparticles into nanocomposite were interpreted through FTIR (Fourier Transform Infrared Spectroscopy) and XPS (X-ray photoelectron spectroscopy) technique, respectively.
34555401	4	84	theme	PVA/CNC/rGO/Ag	734:747	arg1	nanocomposite					749:761	PVA/CNC/rGO/Ag nanocomposite	734:761	PVA/CNC/rGO/Ag nanocomposite	734:761	PVA/CNC/rGO/Ag nanocomposite showed the Ag+ ions sustained release from PVA studied using Inductively Coupled Plasma Mass Spectroscopy (ICP-MS).
34555401	7	85	theme	combined	1425:1432	arg1	effect					1434:1439	the combined effect	1421:1439	the combined effect of CNC, rGO and Ag in PVA matrix	1421:1472	Thus, the combined effect of CNC, rGO and Ag in PVA matrix distinctively resulted into a multifunctional hybrid nanocomposite for potential use in tissue engineering and packaging applications.
34555401	3	86	from	increment	638:646	arg1	modulus					651:657	modulus	651:657	modulus by 184% of PVA	651:672	Uniformly dispersed CNC, rGO and Ag nanoparticles in PVA matrix led to an increment in modulus by 184% of PVA demonstrating the reinforcement outcome of CNC, rGO and Ag.
34555401	6	87	theme	rGO	1380:1382	arg1	combination					1320:1330	a combination	1318:1330	a combination of Ag+ ions (release from Ag nanoparticles) and rGO (having antibacterial effect)	1318:1412	All prepared nanocomposites with different wt% of Ag (PVA, PVA/CNC, PVA/CNC/rGO/Ag) were non-toxic to HEK-293 cell line and exhibited improved antibacterial property against E. coli and S. aureus due to a combination of Ag+ ions (release from Ag nanoparticles) and rGO (having antibacterial effect).
34555401	1	88	theme	silver	269:274	arg1	nanoparticles					281:293	cellulose nanocrystal (CNC), reduced graphene oxide (rGO) and silver (Ag) nanoparticles	207:293	cellulose nanocrystal (CNC), reduced graphene oxide (rGO) and silver (Ag) nanoparticles	207:293	Towards fabricating a hybrid biodegradable multifunctional nanocomposite, cellulose nanocrystal (CNC), reduced graphene oxide (rGO) and silver (Ag) nanoparticles were reinforced into polyvinyl alcohol (PVA) polymer matrix.
33789103	5	0	from	clustering	643:652	arg1	PM					712:713	PM	712:713	PM	712:713	Both VFs regulate actin assembly by tuning the clustering and nucleation activity of formin on the plasma membrane (PM) at the nano-sized scale.
33789103	5	0	from	clustering	643:652	arg1	membrane					702:709	the plasma membrane	691:709	the plasma membrane (PM)	691:714	Both VFs regulate actin assembly by tuning the clustering and nucleation activity of formin on the plasma membrane (PM) at the nano-sized scale.
33789103	1	1	theme	immune	169:174	arg1	responses					176:184	plant immune responses	163:184	plant immune responses	163:184	Plants respond to bacterial infection acutely with actin remodeling during plant immune responses.
33789103	7	2	theme	bacterial	1105:1113	arg1	molecules					1125:1133	bacterial signaling molecules	1105:1133	bacterial signaling molecules	1105:1133	Our results reveal a central mechanism for rapid actin remodeling during plant-bacteria interactions, in which bacterial signaling molecules fine tune plant formin nanoclustering in a host mechanical-structure-dependent manner.
33789103	4	3	theme	pattern-triggered	562:578	arg1	immunity					580:587	pattern-triggered immunity	562:587	pattern-triggered immunity	562:587	campestris (Xcc) diffusible signal factor (DSF), and pathogen-associated molecular pattern (PAMP) flagellin in pattern-triggered immunity (PTI).
33789103	7	4	theme	central	1015:1021	arg1	mechanism					1023:1031	a central mechanism	1013:1031	a central mechanism for rapid actin remodeling during plant-bacteria interactions, in which bacterial signaling molecules fine tune plant formin nanoclustering in a host mechanical-structure-dependent manner	1013:1219	Our results reveal a central mechanism for rapid actin remodeling during plant-bacteria interactions, in which bacterial signaling molecules fine tune plant formin nanoclustering in a host mechanical-structure-dependent manner.
33789103	6	5	theme	cytoskeleton	791:802	arg1	continuum					815:823	the cell-wall-PM-actin cytoskeleton (CW-PM-AC) continuum	768:823	the cell-wall-PM-actin cytoskeleton (CW-PM-AC) continuum	768:823	By being integrated within the cell-wall-PM-actin cytoskeleton (CW-PM-AC) continuum, the dynamic behavior and function of formins are highly dependent on each scaffold layer's composition within the CW-PM-AC continuum during both DSF and PTI signaling.
33789103	3	6	theme	actin	373:377	arg1	remodeling					379:388	actin remodeling	373:388	actin remodeling	373:388	Here, we show that plant-type-I formin serves as the molecular sensor for actin remodeling in response to two bacterial VFs: Xanthomonas campestris pv.
33789103	4	7	theme	signal	479:484	arg1	factor					486:491	diffusible signal factor	468:491	diffusible signal factor (DSF)	468:497	campestris (Xcc) diffusible signal factor (DSF), and pathogen-associated molecular pattern (PAMP) flagellin in pattern-triggered immunity (PTI).
33789103	4	7	theme	signal	479:484	arg1	DSF					494:496	DSF	494:496	DSF	494:496	campestris (Xcc) diffusible signal factor (DSF), and pathogen-associated molecular pattern (PAMP) flagellin in pattern-triggered immunity (PTI).
33789103	6	8	dep	behavior	838:845	arg1	the					826:828	the	826:828	the	826:828	By being integrated within the cell-wall-PM-actin cytoskeleton (CW-PM-AC) continuum, the dynamic behavior and function of formins are highly dependent on each scaffold layer's composition within the CW-PM-AC continuum during both DSF and PTI signaling.
33789103	6	9	theme	cell-wall-PM-actin	772:789	arg1	continuum					815:823	the cell-wall-PM-actin cytoskeleton (CW-PM-AC) continuum	768:823	the cell-wall-PM-actin cytoskeleton (CW-PM-AC) continuum	768:823	By being integrated within the cell-wall-PM-actin cytoskeleton (CW-PM-AC) continuum, the dynamic behavior and function of formins are highly dependent on each scaffold layer's composition within the CW-PM-AC continuum during both DSF and PTI signaling.
33789103	6	10	theme	PTI	979:981	arg1	signaling					983:991	PTI signaling	979:991	PTI signaling	979:991	By being integrated within the cell-wall-PM-actin cytoskeleton (CW-PM-AC) continuum, the dynamic behavior and function of formins are highly dependent on each scaffold layer's composition within the CW-PM-AC continuum during both DSF and PTI signaling.
33789103	3	11	theme	molecular	352:360	arg1	sensor					362:367	the molecular sensor	348:367	the molecular sensor for actin remodeling in response to two bacterial VFs: Xanthomonas campestris pv	348:448	Here, we show that plant-type-I formin serves as the molecular sensor for actin remodeling in response to two bacterial VFs: Xanthomonas campestris pv.
33789103	3	11	theme	molecular	352:360	arg1	formin					331:336	plant-type-I formin	318:336	plant-type-I formin	318:336	Here, we show that plant-type-I formin serves as the molecular sensor for actin remodeling in response to two bacterial VFs: Xanthomonas campestris pv.
33789103	4	12	theme	diffusible	468:477	arg1	factor					486:491	diffusible signal factor	468:491	diffusible signal factor (DSF)	468:497	campestris (Xcc) diffusible signal factor (DSF), and pathogen-associated molecular pattern (PAMP) flagellin in pattern-triggered immunity (PTI).
33789103	4	12	theme	diffusible	468:477	arg1	DSF					494:496	DSF	494:496	DSF	494:496	campestris (Xcc) diffusible signal factor (DSF), and pathogen-associated molecular pattern (PAMP) flagellin in pattern-triggered immunity (PTI).
33789103	7	13	theme	plant-bacteria	1067:1080	arg1	interactions					1082:1093	plant-bacteria interactions	1067:1093	plant-bacteria interactions	1067:1093	Our results reveal a central mechanism for rapid actin remodeling during plant-bacteria interactions, in which bacterial signaling molecules fine tune plant formin nanoclustering in a host mechanical-structure-dependent manner.
33789103	7	14	theme	host	1178:1181	arg1	manner					1214:1219	a host mechanical-structure-dependent manner	1176:1219	a host mechanical-structure-dependent manner	1176:1219	Our results reveal a central mechanism for rapid actin remodeling during plant-bacteria interactions, in which bacterial signaling molecules fine tune plant formin nanoclustering in a host mechanical-structure-dependent manner.
33789103	4	15	from	factor	486:491	arg1	immunity					580:587	pattern-triggered immunity	562:587	pattern-triggered immunity	562:587	campestris (Xcc) diffusible signal factor (DSF), and pathogen-associated molecular pattern (PAMP) flagellin in pattern-triggered immunity (PTI).
33789103	7	16	theme	mechanical-structure-dependent	1183:1212	arg1	manner					1214:1219	a host mechanical-structure-dependent manner	1176:1219	a host mechanical-structure-dependent manner	1176:1219	Our results reveal a central mechanism for rapid actin remodeling during plant-bacteria interactions, in which bacterial signaling molecules fine tune plant formin nanoclustering in a host mechanical-structure-dependent manner.
33789103	5	17	dep	clustering	643:652	arg1	the					639:641	the	639:641	the	639:641	Both VFs regulate actin assembly by tuning the clustering and nucleation activity of formin on the plasma membrane (PM) at the nano-sized scale.
33789103	3	18	dep	VFs	419:421	arg1	pv					447:448	pv	447:448	two bacterial VFs: Xanthomonas campestris pv	405:448	Here, we show that plant-type-I formin serves as the molecular sensor for actin remodeling in response to two bacterial VFs: Xanthomonas campestris pv.
33789103	6	19	theme	formins	863:869	arg1	function					851:858	function	851:858	function	851:858	By being integrated within the cell-wall-PM-actin cytoskeleton (CW-PM-AC) continuum, the dynamic behavior and function of formins are highly dependent on each scaffold layer's composition within the CW-PM-AC continuum during both DSF and PTI signaling.
33789103	6	19	theme	formins	863:869	arg1	behavior					838:845	dynamic behavior	830:845	dynamic behavior	830:845	By being integrated within the cell-wall-PM-actin cytoskeleton (CW-PM-AC) continuum, the dynamic behavior and function of formins are highly dependent on each scaffold layer's composition within the CW-PM-AC continuum during both DSF and PTI signaling.
33789103	4	20	from	flagellin	549:557	arg1	immunity					580:587	pattern-triggered immunity	562:587	pattern-triggered immunity	562:587	campestris (Xcc) diffusible signal factor (DSF), and pathogen-associated molecular pattern (PAMP) flagellin in pattern-triggered immunity (PTI).
33789103	7	21	theme	plant	1145:1149	arg1	nanoclustering					1158:1171	plant formin nanoclustering	1145:1171	plant formin nanoclustering	1145:1171	Our results reveal a central mechanism for rapid actin remodeling during plant-bacteria interactions, in which bacterial signaling molecules fine tune plant formin nanoclustering in a host mechanical-structure-dependent manner.
33789103	4	22	theme	PAMP	543:546	arg1	PTI					590:592	PTI	590:592	PTI	590:592	campestris (Xcc) diffusible signal factor (DSF), and pathogen-associated molecular pattern (PAMP) flagellin in pattern-triggered immunity (PTI).
33789103	4	22	theme	PAMP	543:546	arg1	flagellin					549:557	pathogen-associated molecular pattern (PAMP) flagellin	504:557	pathogen-associated molecular pattern (PAMP) flagellin in pattern-triggered immunity (PTI)	504:593	campestris (Xcc) diffusible signal factor (DSF), and pathogen-associated molecular pattern (PAMP) flagellin in pattern-triggered immunity (PTI).
33789103	7	23	theme	rapid	1037:1041	arg1	remodeling					1049:1058	rapid actin remodeling	1037:1058	rapid actin remodeling during plant-bacteria interactions, in which bacterial signaling molecules fine tune plant formin nanoclustering in a host mechanical-structure-dependent manner	1037:1219	Our results reveal a central mechanism for rapid actin remodeling during plant-bacteria interactions, in which bacterial signaling molecules fine tune plant formin nanoclustering in a host mechanical-structure-dependent manner.
33789103	1	24	theme	bacterial	106:114	arg1	infection					116:124	bacterial infection	106:124	bacterial infection	106:124	Plants respond to bacterial infection acutely with actin remodeling during plant immune responses.
33789103	0	25	theme	actin	31:35	arg1	assembly					37:44	actin assembly	31:44	actin assembly	31:44	Formin nanoclustering-mediated actin assembly during plant flagellin and DSF signaling.
33789103	7	26	theme	actin	1043:1047	arg1	remodeling					1049:1058	rapid actin remodeling	1037:1058	rapid actin remodeling during plant-bacteria interactions, in which bacterial signaling molecules fine tune plant formin nanoclustering in a host mechanical-structure-dependent manner	1037:1219	Our results reveal a central mechanism for rapid actin remodeling during plant-bacteria interactions, in which bacterial signaling molecules fine tune plant formin nanoclustering in a host mechanical-structure-dependent manner.
33789103	6	27	theme	CW-PM-AC	940:947	arg1	continuum					949:957	the CW-PM-AC continuum	936:957	the CW-PM-AC continuum during both DSF and PTI signaling	936:991	By being integrated within the cell-wall-PM-actin cytoskeleton (CW-PM-AC) continuum, the dynamic behavior and function of formins are highly dependent on each scaffold layer's composition within the CW-PM-AC continuum during both DSF and PTI signaling.
33789103	0	28	theme	plant	53:57	arg1	flagellin					59:67	plant flagellin	53:67	plant flagellin	53:67	Formin nanoclustering-mediated actin assembly during plant flagellin and DSF signaling.
33789103	5	29	theme	plasma	695:700	arg1	PM					712:713	PM	712:713	PM	712:713	Both VFs regulate actin assembly by tuning the clustering and nucleation activity of formin on the plasma membrane (PM) at the nano-sized scale.
33789103	5	29	theme	plasma	695:700	arg1	membrane					702:709	the plasma membrane	691:709	the plasma membrane (PM)	691:714	Both VFs regulate actin assembly by tuning the clustering and nucleation activity of formin on the plasma membrane (PM) at the nano-sized scale.
33789103	3	30	from	sensor	362:367	arg1	response					393:400	response	393:400	response to two bacterial VFs: Xanthomonas campestris pv	393:448	Here, we show that plant-type-I formin serves as the molecular sensor for actin remodeling in response to two bacterial VFs: Xanthomonas campestris pv.
33789103	4	31	theme	molecular	524:532	arg1	PTI					590:592	PTI	590:592	PTI	590:592	campestris (Xcc) diffusible signal factor (DSF), and pathogen-associated molecular pattern (PAMP) flagellin in pattern-triggered immunity (PTI).
33789103	4	31	theme	molecular	524:532	arg1	flagellin					549:557	pathogen-associated molecular pattern (PAMP) flagellin	504:557	pathogen-associated molecular pattern (PAMP) flagellin in pattern-triggered immunity (PTI)	504:593	campestris (Xcc) diffusible signal factor (DSF), and pathogen-associated molecular pattern (PAMP) flagellin in pattern-triggered immunity (PTI).
33789103	6	32	theme	dynamic	830:836	arg1	behavior					838:845	dynamic behavior	830:845	dynamic behavior	830:845	By being integrated within the cell-wall-PM-actin cytoskeleton (CW-PM-AC) continuum, the dynamic behavior and function of formins are highly dependent on each scaffold layer's composition within the CW-PM-AC continuum during both DSF and PTI signaling.
33789103	6	33	theme	scaffold	900:907	arg1	layer					909:913	each scaffold layer's	895:915	each scaffold layer's composition within the CW-PM-AC continuum during both DSF and PTI signaling	895:991	By being integrated within the cell-wall-PM-actin cytoskeleton (CW-PM-AC) continuum, the dynamic behavior and function of formins are highly dependent on each scaffold layer's composition within the CW-PM-AC continuum during both DSF and PTI signaling.
33789103	7	34	theme	signaling	1115:1123	arg1	molecules					1125:1133	bacterial signaling molecules	1105:1133	bacterial signaling molecules	1105:1133	Our results reveal a central mechanism for rapid actin remodeling during plant-bacteria interactions, in which bacterial signaling molecules fine tune plant formin nanoclustering in a host mechanical-structure-dependent manner.
33789103	4	35	theme	pathogen-associated	504:522	arg1	PTI					590:592	PTI	590:592	PTI	590:592	campestris (Xcc) diffusible signal factor (DSF), and pathogen-associated molecular pattern (PAMP) flagellin in pattern-triggered immunity (PTI).
33789103	4	35	theme	pathogen-associated	504:522	arg1	flagellin					549:557	pathogen-associated molecular pattern (PAMP) flagellin	504:557	pathogen-associated molecular pattern (PAMP) flagellin in pattern-triggered immunity (PTI)	504:593	campestris (Xcc) diffusible signal factor (DSF), and pathogen-associated molecular pattern (PAMP) flagellin in pattern-triggered immunity (PTI).
33789103	5	36	theme	nucleation	658:667	arg1	activity					669:676	nucleation activity	658:676	nucleation activity	658:676	Both VFs regulate actin assembly by tuning the clustering and nucleation activity of formin on the plasma membrane (PM) at the nano-sized scale.
33789103	4	37	dep	factor	486:491	arg1	Xcc					463:465	Xcc	463:465	Xcc	463:465	campestris (Xcc) diffusible signal factor (DSF), and pathogen-associated molecular pattern (PAMP) flagellin in pattern-triggered immunity (PTI).
33789103	4	37	dep	factor	486:491	arg1	campestris					451:460	campestris	451:460	campestris (Xcc)	451:466	campestris (Xcc) diffusible signal factor (DSF), and pathogen-associated molecular pattern (PAMP) flagellin in pattern-triggered immunity (PTI).
33789103	3	38	theme	bacterial	409:417	arg1	VFs					419:421	two bacterial VFs	405:421	two bacterial VFs: Xanthomonas campestris pv	405:448	Here, we show that plant-type-I formin serves as the molecular sensor for actin remodeling in response to two bacterial VFs: Xanthomonas campestris pv.
33789103	3	39	theme	plant-type-I	318:329	arg1	sensor					362:367	the molecular sensor	348:367	the molecular sensor for actin remodeling in response to two bacterial VFs: Xanthomonas campestris pv	348:448	Here, we show that plant-type-I formin serves as the molecular sensor for actin remodeling in response to two bacterial VFs: Xanthomonas campestris pv.
33789103	3	39	theme	plant-type-I	318:329	arg1	formin					331:336	plant-type-I formin	318:336	plant-type-I formin	318:336	Here, we show that plant-type-I formin serves as the molecular sensor for actin remodeling in response to two bacterial VFs: Xanthomonas campestris pv.
33789103	5	40	from	scale	734:738	arg1	tuning					632:637	tuning	632:637	tuning	632:637	Both VFs regulate actin assembly by tuning the clustering and nucleation activity of formin on the plasma membrane (PM) at the nano-sized scale.
33789103	5	41	theme	actin	614:618	arg1	assembly					620:627	actin assembly	614:627	actin assembly	614:627	Both VFs regulate actin assembly by tuning the clustering and nucleation activity of formin on the plasma membrane (PM) at the nano-sized scale.
33789103	7	42	theme	formin	1151:1156	arg1	nanoclustering					1158:1171	plant formin nanoclustering	1145:1171	plant formin nanoclustering	1145:1171	Our results reveal a central mechanism for rapid actin remodeling during plant-bacteria interactions, in which bacterial signaling molecules fine tune plant formin nanoclustering in a host mechanical-structure-dependent manner.
33789103	4	43	theme	pattern	534:540	arg1	PTI					590:592	PTI	590:592	PTI	590:592	campestris (Xcc) diffusible signal factor (DSF), and pathogen-associated molecular pattern (PAMP) flagellin in pattern-triggered immunity (PTI).
33789103	4	43	theme	pattern	534:540	arg1	flagellin					549:557	pathogen-associated molecular pattern (PAMP) flagellin	504:557	pathogen-associated molecular pattern (PAMP) flagellin in pattern-triggered immunity (PTI)	504:593	campestris (Xcc) diffusible signal factor (DSF), and pathogen-associated molecular pattern (PAMP) flagellin in pattern-triggered immunity (PTI).
33789103	2	44	theme	virulence	221:229	arg1	VFs					240:242	VFs	240:242	VFs	240:242	The mechanisms by which bacterial virulence factors (VFs) modulate plant actin polymerization remain enigmatic.
33789103	2	44	theme	virulence	221:229	arg1	factors					231:237	bacterial virulence factors	211:237	bacterial virulence factors (VFs)	211:243	The mechanisms by which bacterial virulence factors (VFs) modulate plant actin polymerization remain enigmatic.
33789103	5	45	theme	nano-sized	723:732	arg1	scale					734:738	the nano-sized scale	719:738	the nano-sized scale	719:738	Both VFs regulate actin assembly by tuning the clustering and nucleation activity of formin on the plasma membrane (PM) at the nano-sized scale.
33789103	1	46	theme	actin	139:143	arg1	remodeling					145:154	actin remodeling	139:154	actin remodeling during plant immune responses	139:184	Plants respond to bacterial infection acutely with actin remodeling during plant immune responses.
33789103	5	47	theme	formin	681:686	arg1	clustering					643:652	clustering	643:652	clustering	643:652	Both VFs regulate actin assembly by tuning the clustering and nucleation activity of formin on the plasma membrane (PM) at the nano-sized scale.
33789103	5	47	theme	formin	681:686	arg1	activity					669:676	nucleation activity	658:676	nucleation activity	658:676	Both VFs regulate actin assembly by tuning the clustering and nucleation activity of formin on the plasma membrane (PM) at the nano-sized scale.
33789103	2	48	theme	bacterial	211:219	arg1	VFs					240:242	VFs	240:242	VFs	240:242	The mechanisms by which bacterial virulence factors (VFs) modulate plant actin polymerization remain enigmatic.
33789103	2	48	theme	bacterial	211:219	arg1	factors					231:237	bacterial virulence factors	211:237	bacterial virulence factors (VFs)	211:243	The mechanisms by which bacterial virulence factors (VFs) modulate plant actin polymerization remain enigmatic.
33789103	6	49	theme	CW-PM-AC	805:812	arg1	continuum					815:823	the cell-wall-PM-actin cytoskeleton (CW-PM-AC) continuum	768:823	the cell-wall-PM-actin cytoskeleton (CW-PM-AC) continuum	768:823	By being integrated within the cell-wall-PM-actin cytoskeleton (CW-PM-AC) continuum, the dynamic behavior and function of formins are highly dependent on each scaffold layer's composition within the CW-PM-AC continuum during both DSF and PTI signaling.
33789103	2	50	theme	actin	260:264	arg1	polymerization					266:279	plant actin polymerization	254:279	plant actin polymerization	254:279	The mechanisms by which bacterial virulence factors (VFs) modulate plant actin polymerization remain enigmatic.
33789103	0	51	theme	DSF	73:75	arg1	signaling					77:85	DSF signaling	73:85	DSF signaling	73:85	Formin nanoclustering-mediated actin assembly during plant flagellin and DSF signaling.
33789103	2	52	theme	plant	254:258	arg1	polymerization					266:279	plant actin polymerization	254:279	plant actin polymerization	254:279	The mechanisms by which bacterial virulence factors (VFs) modulate plant actin polymerization remain enigmatic.
33789103	1	53	theme	plant	163:167	arg1	responses					176:184	plant immune responses	163:184	plant immune responses	163:184	Plants respond to bacterial infection acutely with actin remodeling during plant immune responses.
33789103	5	54	from	activity	669:676	arg1	PM					712:713	PM	712:713	PM	712:713	Both VFs regulate actin assembly by tuning the clustering and nucleation activity of formin on the plasma membrane (PM) at the nano-sized scale.
33789103	5	54	from	activity	669:676	arg1	membrane					702:709	the plasma membrane	691:709	the plasma membrane (PM)	691:714	Both VFs regulate actin assembly by tuning the clustering and nucleation activity of formin on the plasma membrane (PM) at the nano-sized scale.
35068098	7	0	theme	abundance	1446:1454	arg1	estimates					1456:1464	abundance estimates	1446:1464	abundance estimates by C. perfringens 16S	1446:1486	When data from all time points were combined, abundance estimates by C. perfringens 16S were statistically equivalent (α = 0.10) to both C. perfringens PCR and C. perfringens counts.
35068098	7	1	dep	C.	1560:1561	arg1	perfringens					1563:1573	perfringens	1563:1573	perfringens	1563:1573	When data from all time points were combined, abundance estimates by C. perfringens 16S were statistically equivalent (α = 0.10) to both C. perfringens PCR and C. perfringens counts.
35068098	5	2	dep	Clostridium	1001:1011	arg1	perfringens					1013:1023	perfringens	1013:1023	perfringens	1013:1023	Clostridium perfringens counts on select agar and semiquantitative PCR for C. perfringens were compared with 16S analysis for equivalence testing.
35068098	6	3	theme	Tpel	1249:1252	arg1	DNA					1260:1262	Tpel toxin DNA	1249:1262	Tpel toxin DNA	1249:1262	Relative abundance of C. perfringens estimated by 16S analysis and semiquantitative PCR for netB and Tpel toxin DNA were analyzed by Pearson linear correlation and statistical equivalence analyses with cumulative chick mortality at 4 and 9 wk growout.
35068098	10	4	theme	P	2064:2064	arg1	<					2066:2066	P < 0.05	2064:2071	P < 0.05	2064:2071	Similarly, abundance of Tpel at 0 and 2 wk showed a linear relationship with cumulative percent mortality at both 4 and 9 wk growout (0.44 ≤ r ≤ 0.54, P < 0.05).
35068098	6	5	theme	Relative	1148:1155	arg1	abundance					1157:1165	Relative abundance	1148:1165	Relative abundance of C. perfringens estimated by 16S analysis and semiquantitative PCR for netB and Tpel toxin DNA	1148:1262	Relative abundance of C. perfringens estimated by 16S analysis and semiquantitative PCR for netB and Tpel toxin DNA were analyzed by Pearson linear correlation and statistical equivalence analyses with cumulative chick mortality at 4 and 9 wk growout.
35068098	8	6	theme	C.	1642:1643	arg1	abundance					1657:1665	C. perfringens abundance	1642:1665	C. perfringens abundance	1642:1665	Yet, no correlations were observed between any estimate of C. perfringens abundance and cumulative percent chick mortality at 4 or 9 wk growout.
35068098	1	7	theme	bacterial	258:266	arg1	RNA					282:284	bacterial 16S ribosomal RNA	258:284	bacterial 16S ribosomal RNA (rRNA) in poultry litter	258:309	The purpose of this study was twofold-first, to determine whether analysis of bacterial 16S ribosomal RNA (rRNA) in poultry litter corroborated standard Clostridium perfringens counts and PCR assay, and second, to find whether a correlation between 16S rRNA analysis and netB or Tpel toxin PCR intensity with chick mortality existed.
35068098	1	7	theme	bacterial	258:266	arg1	rRNA					287:290	rRNA	287:290	rRNA	287:290	The purpose of this study was twofold-first, to determine whether analysis of bacterial 16S ribosomal RNA (rRNA) in poultry litter corroborated standard Clostridium perfringens counts and PCR assay, and second, to find whether a correlation between 16S rRNA analysis and netB or Tpel toxin PCR intensity with chick mortality existed.
35068098	9	8	theme	=	1832:1832	arg1	0.55					1834:1837	r = 0.55	1830:1837	r = 0.55	1830:1837	However, correlation analyses revealed a significant linear relationship between netB signal at 0 wk (r = 0.55) and 4 wk (r = 0.46) and cumulative mortality at 9 wk growout (P < 0.05).
35068098	9	8	theme	=	1832:1832	arg1	wk					1826:1827	0 wk	1824:1827	0 wk (r = 0.55)	1824:1838	However, correlation analyses revealed a significant linear relationship between netB signal at 0 wk (r = 0.55) and 4 wk (r = 0.46) and cumulative mortality at 9 wk growout (P < 0.05).
35068098	1	9	theme	rRNA	433:436	arg1	analysis					438:445	16S rRNA analysis	429:445	16S rRNA analysis	429:445	The purpose of this study was twofold-first, to determine whether analysis of bacterial 16S ribosomal RNA (rRNA) in poultry litter corroborated standard Clostridium perfringens counts and PCR assay, and second, to find whether a correlation between 16S rRNA analysis and netB or Tpel toxin PCR intensity with chick mortality existed.
35068098	1	10	theme	16S	268:270	arg1	RNA					282:284	bacterial 16S ribosomal RNA	258:284	bacterial 16S ribosomal RNA (rRNA) in poultry litter	258:309	The purpose of this study was twofold-first, to determine whether analysis of bacterial 16S ribosomal RNA (rRNA) in poultry litter corroborated standard Clostridium perfringens counts and PCR assay, and second, to find whether a correlation between 16S rRNA analysis and netB or Tpel toxin PCR intensity with chick mortality existed.
35068098	1	10	theme	16S	268:270	arg1	rRNA					287:290	rRNA	287:290	rRNA	287:290	The purpose of this study was twofold-first, to determine whether analysis of bacterial 16S ribosomal RNA (rRNA) in poultry litter corroborated standard Clostridium perfringens counts and PCR assay, and second, to find whether a correlation between 16S rRNA analysis and netB or Tpel toxin PCR intensity with chick mortality existed.
35068098	2	11	theme	control	670:676	arg1	program					678:684	a coccidiosis vaccine control program	648:684	a coccidiosis vaccine control program	648:684	At three time points of growout (0, 2, and 4 wk) litter samples were collected from 23 broiler houses representing eight farms during a coccidiosis vaccine control program.
35068098	10	12	with	relationship	1972:1983	arg1	mortality					2009:2017	cumulative percent mortality	1990:2017	cumulative percent mortality at both 4 and 9 wk growout (0.44 ≤ r ≤ 0.54, P < 0.05)	1990:2072	Similarly, abundance of Tpel at 0 and 2 wk showed a linear relationship with cumulative percent mortality at both 4 and 9 wk growout (0.44 ≤ r ≤ 0.54, P < 0.05).
35068098	8	13	theme	cumulative	1671:1680	arg1	mortality					1696:1704	cumulative percent chick mortality	1671:1704	cumulative percent chick mortality	1671:1704	Yet, no correlations were observed between any estimate of C. perfringens abundance and cumulative percent chick mortality at 4 or 9 wk growout.
35068098	10	14	theme	r	2054:2054	arg1	wk					2035:2036	4 and 9 wk growout	2027:2044	wk	2035:2036	Similarly, abundance of Tpel at 0 and 2 wk showed a linear relationship with cumulative percent mortality at both 4 and 9 wk growout (0.44 ≤ r ≤ 0.54, P < 0.05).
35068098	10	14	theme	r	2054:2054	arg1	0.54					2058:2061	0.44 ≤ r ≤ 0.54	2047:2061	0.44 ≤ r ≤ 0.54	2047:2061	Similarly, abundance of Tpel at 0 and 2 wk showed a linear relationship with cumulative percent mortality at both 4 and 9 wk growout (0.44 ≤ r ≤ 0.54, P < 0.05).
35068098	8	15	theme	chick	1690:1694	arg1	mortality					1696:1704	cumulative percent chick mortality	1671:1704	cumulative percent chick mortality	1671:1704	Yet, no correlations were observed between any estimate of C. perfringens abundance and cumulative percent chick mortality at 4 or 9 wk growout.
35068098	0	16	from	Counts	90:95	arg1	Agar					107:110	Select Agar	100:110	Select Agar	100:110	Metagenomic Analysis of 16S Clostridium perfringens Amplicons Corroborates C. perfringens Counts on Select Agar and C. perfringens PCR Analyses of Bacteria in Broiler Farm Litter.
35068098	9	17	from	wk	1846:1847	arg1	signal					1814:1819	netB signal	1809:1819	netB signal at 0 wk (r = 0.55) and 4 wk (r = 0.46)	1809:1858	However, correlation analyses revealed a significant linear relationship between netB signal at 0 wk (r = 0.55) and 4 wk (r = 0.46) and cumulative mortality at 9 wk growout (P < 0.05).
35068098	8	18	from	wk	1716:1717	arg1	mortality					1696:1704	cumulative percent chick mortality	1671:1704	cumulative percent chick mortality	1671:1704	Yet, no correlations were observed between any estimate of C. perfringens abundance and cumulative percent chick mortality at 4 or 9 wk growout.
35068098	8	18	from	wk	1716:1717	arg1	abundance					1657:1665	C. perfringens abundance	1642:1665	C. perfringens abundance	1642:1665	Yet, no correlations were observed between any estimate of C. perfringens abundance and cumulative percent chick mortality at 4 or 9 wk growout.
35068098	1	19	from	analysis	246:253	arg1	litter					304:309	poultry litter	296:309	poultry litter	296:309	The purpose of this study was twofold-first, to determine whether analysis of bacterial 16S ribosomal RNA (rRNA) in poultry litter corroborated standard Clostridium perfringens counts and PCR assay, and second, to find whether a correlation between 16S rRNA analysis and netB or Tpel toxin PCR intensity with chick mortality existed.
35068098	5	20	theme	select	1035:1040	arg1	agar					1042:1045	select agar	1035:1045	select agar	1035:1045	Clostridium perfringens counts on select agar and semiquantitative PCR for C. perfringens were compared with 16S analysis for equivalence testing.
35068098	9	21	theme	correlation	1737:1747	arg1	analyses					1749:1756	correlation analyses	1737:1756	correlation analyses	1737:1756	However, correlation analyses revealed a significant linear relationship between netB signal at 0 wk (r = 0.55) and 4 wk (r = 0.46) and cumulative mortality at 9 wk growout (P < 0.05).
35068098	7	22	theme	C.	1560:1561	arg1	counts					1575:1580	C. perfringens counts	1560:1580	C. perfringens counts	1560:1580	When data from all time points were combined, abundance estimates by C. perfringens 16S were statistically equivalent (α = 0.10) to both C. perfringens PCR and C. perfringens counts.
35068098	2	23	theme	growout	538:544	arg1	points					528:533	three time points	517:533	three time points of growout (0, 2, and 4 wk)	517:561	At three time points of growout (0, 2, and 4 wk) litter samples were collected from 23 broiler houses representing eight farms during a coccidiosis vaccine control program.
35068098	9	24	theme	=	1852:1852	arg1	r					1850:1850	r = 0.46	1850:1857	r = 0.46	1850:1857	However, correlation analyses revealed a significant linear relationship between netB signal at 0 wk (r = 0.55) and 4 wk (r = 0.46) and cumulative mortality at 9 wk growout (P < 0.05).
35068098	9	24	theme	=	1852:1852	arg1	wk					1846:1847	4 wk	1844:1847	4 wk (r = 0.46)	1844:1858	However, correlation analyses revealed a significant linear relationship between netB signal at 0 wk (r = 0.55) and 4 wk (r = 0.46) and cumulative mortality at 9 wk growout (P < 0.05).
35068098	0	25	theme	Bacteria	147:154	arg1	Counts					90:95	C. perfringens Counts	75:95	C. perfringens Counts on Select Agar	75:110	Metagenomic Analysis of 16S Clostridium perfringens Amplicons Corroborates C. perfringens Counts on Select Agar and C. perfringens PCR Analyses of Bacteria in Broiler Farm Litter.
35068098	0	25	theme	Bacteria	147:154	arg1	Analyses					135:142	C. perfringens PCR Analyses	116:142	C. perfringens PCR Analyses of Bacteria	116:154	Metagenomic Analysis of 16S Clostridium perfringens Amplicons Corroborates C. perfringens Counts on Select Agar and C. perfringens PCR Analyses of Bacteria in Broiler Farm Litter.
35068098	11	26	theme	percent	2174:2180	arg1	mortality					2188:2196	cumulative percent chick mortality	2163:2196	cumulative percent chick mortality	2163:2196	No correlations were observed between any other genera or species determined by 16S and cumulative percent chick mortality.
35068098	6	27	theme	perfringens	1173:1183	arg1	abundance					1157:1165	Relative abundance	1148:1165	Relative abundance of C. perfringens estimated by 16S analysis and semiquantitative PCR for netB and Tpel toxin DNA	1148:1262	Relative abundance of C. perfringens estimated by 16S analysis and semiquantitative PCR for netB and Tpel toxin DNA were analyzed by Pearson linear correlation and statistical equivalence analyses with cumulative chick mortality at 4 and 9 wk growout.
35068098	10	28	theme	cumulative	1990:1999	arg1	mortality					2009:2017	cumulative percent mortality	1990:2017	cumulative percent mortality at both 4 and 9 wk growout (0.44 ≤ r ≤ 0.54, P < 0.05)	1990:2072	Similarly, abundance of Tpel at 0 and 2 wk showed a linear relationship with cumulative percent mortality at both 4 and 9 wk growout (0.44 ≤ r ≤ 0.54, P < 0.05).
35068098	0	29	theme	Broiler	159:165	arg1	Litter					172:177	Broiler Farm Litter	159:177	Broiler Farm Litter	159:177	Metagenomic Analysis of 16S Clostridium perfringens Amplicons Corroborates C. perfringens Counts on Select Agar and C. perfringens PCR Analyses of Bacteria in Broiler Farm Litter.
35068098	5	30	theme	Clostridium	1001:1011	arg1	counts					1025:1030	Clostridium perfringens counts	1001:1030	Clostridium perfringens counts on select agar and semiquantitative PCR for C. perfringens	1001:1089	Clostridium perfringens counts on select agar and semiquantitative PCR for C. perfringens were compared with 16S analysis for equivalence testing.
35068098	9	31	from	wk	1826:1827	arg1	signal					1814:1819	netB signal	1809:1819	netB signal at 0 wk (r = 0.55) and 4 wk (r = 0.46)	1809:1858	However, correlation analyses revealed a significant linear relationship between netB signal at 0 wk (r = 0.55) and 4 wk (r = 0.46) and cumulative mortality at 9 wk growout (P < 0.05).
35068098	3	32	theme	hypervariable	776:788	arg1	region					796:801	the hypervariable V3-V4 region	772:801	the hypervariable V3-V4 region of bacterial 16s rRNA	772:823	DNA extracted from these samples was used for microbiota determination by sequencing the hypervariable V3-V4 region of bacterial 16s rRNA.
35068098	7	33	theme	C.	1537:1538	arg1	PCR					1552:1554	C. perfringens PCR	1537:1554	C. perfringens PCR	1537:1554	When data from all time points were combined, abundance estimates by C. perfringens 16S were statistically equivalent (α = 0.10) to both C. perfringens PCR and C. perfringens counts.
35068098	2	34	theme	coccidiosis	650:660	arg1	program					678:684	a coccidiosis vaccine control program	648:684	a coccidiosis vaccine control program	648:684	At three time points of growout (0, 2, and 4 wk) litter samples were collected from 23 broiler houses representing eight farms during a coccidiosis vaccine control program.
35068098	0	35	theme	C.	75:76	arg1	Counts					90:95	C. perfringens Counts	75:95	C. perfringens Counts on Select Agar	75:110	Metagenomic Analysis of 16S Clostridium perfringens Amplicons Corroborates C. perfringens Counts on Select Agar and C. perfringens PCR Analyses of Bacteria in Broiler Farm Litter.
35068098	5	36	theme	C.	1076:1077	arg1	perfringens					1079:1089	C. perfringens	1076:1089	C. perfringens	1076:1089	Clostridium perfringens counts on select agar and semiquantitative PCR for C. perfringens were compared with 16S analysis for equivalence testing.
35068098	3	37	theme	rRNA	820:823	arg1	region					796:801	the hypervariable V3-V4 region	772:801	the hypervariable V3-V4 region of bacterial 16s rRNA	772:823	DNA extracted from these samples was used for microbiota determination by sequencing the hypervariable V3-V4 region of bacterial 16s rRNA.
35068098	4	38	theme	relative	928:935	arg1	abundance					937:945	relative abundance	928:945	relative abundance	928:945	Obtained sequences were analyzed by QIIME 2 and the Greengenes database for taxonomic composition and relative abundance of C. perfringens in the litter bacterial population.
35068098	6	39	theme	cumulative	1350:1359	arg1	mortality					1367:1375	cumulative chick mortality	1350:1375	cumulative chick mortality	1350:1375	Relative abundance of C. perfringens estimated by 16S analysis and semiquantitative PCR for netB and Tpel toxin DNA were analyzed by Pearson linear correlation and statistical equivalence analyses with cumulative chick mortality at 4 and 9 wk growout.
35068098	1	40	theme	standard	324:331	arg1	counts					357:362	standard Clostridium perfringens counts	324:362	standard Clostridium perfringens counts	324:362	The purpose of this study was twofold-first, to determine whether analysis of bacterial 16S ribosomal RNA (rRNA) in poultry litter corroborated standard Clostridium perfringens counts and PCR assay, and second, to find whether a correlation between 16S rRNA analysis and netB or Tpel toxin PCR intensity with chick mortality existed.
35068098	6	41	theme	equivalence	1324:1334	arg1	analyses					1336:1343	statistical equivalence analyses	1312:1343	statistical equivalence analyses with cumulative chick mortality	1312:1375	Relative abundance of C. perfringens estimated by 16S analysis and semiquantitative PCR for netB and Tpel toxin DNA were analyzed by Pearson linear correlation and statistical equivalence analyses with cumulative chick mortality at 4 and 9 wk growout.
35068098	3	42	theme	bacterial	806:814	arg1	rRNA					820:823	bacterial 16s rRNA	806:823	bacterial 16s rRNA	806:823	DNA extracted from these samples was used for microbiota determination by sequencing the hypervariable V3-V4 region of bacterial 16s rRNA.
35068098	4	43	theme	Greengenes	878:887	arg1	database					889:896	the Greengenes database	874:896	the Greengenes database	874:896	Obtained sequences were analyzed by QIIME 2 and the Greengenes database for taxonomic composition and relative abundance of C. perfringens in the litter bacterial population.
35068098	0	44	theme	Select	100:105	arg1	Agar					107:110	Select Agar	100:110	Select Agar	100:110	Metagenomic Analysis of 16S Clostridium perfringens Amplicons Corroborates C. perfringens Counts on Select Agar and C. perfringens PCR Analyses of Bacteria in Broiler Farm Litter.
35068098	10	45	dep	0.54	2058:2061	arg1	<					2066:2066	P < 0.05	2064:2071	P < 0.05	2064:2071	Similarly, abundance of Tpel at 0 and 2 wk showed a linear relationship with cumulative percent mortality at both 4 and 9 wk growout (0.44 ≤ r ≤ 0.54, P < 0.05).
35068098	10	46	theme	growout	2038:2044	arg1	wk					2035:2036	4 and 9 wk growout	2027:2044	wk	2035:2036	Similarly, abundance of Tpel at 0 and 2 wk showed a linear relationship with cumulative percent mortality at both 4 and 9 wk growout (0.44 ≤ r ≤ 0.54, P < 0.05).
35068098	10	46	theme	growout	2038:2044	arg1	0.54					2058:2061	0.44 ≤ r ≤ 0.54	2047:2061	0.44 ≤ r ≤ 0.54	2047:2061	Similarly, abundance of Tpel at 0 and 2 wk showed a linear relationship with cumulative percent mortality at both 4 and 9 wk growout (0.44 ≤ r ≤ 0.54, P < 0.05).
35068098	6	47	theme	statistical	1312:1322	arg1	analyses					1336:1343	statistical equivalence analyses	1312:1343	statistical equivalence analyses with cumulative chick mortality	1312:1375	Relative abundance of C. perfringens estimated by 16S analysis and semiquantitative PCR for netB and Tpel toxin DNA were analyzed by Pearson linear correlation and statistical equivalence analyses with cumulative chick mortality at 4 and 9 wk growout.
35068098	10	48	theme	≤	2056:2056	arg1	wk					2035:2036	4 and 9 wk growout	2027:2044	wk	2035:2036	Similarly, abundance of Tpel at 0 and 2 wk showed a linear relationship with cumulative percent mortality at both 4 and 9 wk growout (0.44 ≤ r ≤ 0.54, P < 0.05).
35068098	10	48	theme	≤	2056:2056	arg1	0.54					2058:2061	0.44 ≤ r ≤ 0.54	2047:2061	0.44 ≤ r ≤ 0.54	2047:2061	Similarly, abundance of Tpel at 0 and 2 wk showed a linear relationship with cumulative percent mortality at both 4 and 9 wk growout (0.44 ≤ r ≤ 0.54, P < 0.05).
35068098	0	49	theme	Metagenomic	0:10	arg1	Analysis					12:19	Metagenomic Analysis	0:19	Metagenomic Analysis of 16S Clostridium perfringens Amplicons	0:60	Metagenomic Analysis of 16S Clostridium perfringens Amplicons Corroborates C. perfringens Counts on Select Agar and C. perfringens PCR Analyses of Bacteria in Broiler Farm Litter.
35068098	5	50	theme	equivalence	1127:1137	arg1	testing					1139:1145	equivalence testing	1127:1145	equivalence testing	1127:1145	Clostridium perfringens counts on select agar and semiquantitative PCR for C. perfringens were compared with 16S analysis for equivalence testing.
35068098	4	51	theme	bacterial	979:987	arg1	population					989:998	the litter bacterial population	968:998	the litter bacterial population	968:998	Obtained sequences were analyzed by QIIME 2 and the Greengenes database for taxonomic composition and relative abundance of C. perfringens in the litter bacterial population.
35068098	1	52	dep	Clostridium	333:343	arg1	perfringens					345:355	perfringens	345:355	perfringens	345:355	The purpose of this study was twofold-first, to determine whether analysis of bacterial 16S ribosomal RNA (rRNA) in poultry litter corroborated standard Clostridium perfringens counts and PCR assay, and second, to find whether a correlation between 16S rRNA analysis and netB or Tpel toxin PCR intensity with chick mortality existed.
35068098	10	53	theme	≤	2052:2052	arg1	wk					2035:2036	4 and 9 wk growout	2027:2044	wk	2035:2036	Similarly, abundance of Tpel at 0 and 2 wk showed a linear relationship with cumulative percent mortality at both 4 and 9 wk growout (0.44 ≤ r ≤ 0.54, P < 0.05).
35068098	10	53	theme	≤	2052:2052	arg1	0.54					2058:2061	0.44 ≤ r ≤ 0.54	2047:2061	0.44 ≤ r ≤ 0.54	2047:2061	Similarly, abundance of Tpel at 0 and 2 wk showed a linear relationship with cumulative percent mortality at both 4 and 9 wk growout (0.44 ≤ r ≤ 0.54, P < 0.05).
35068098	6	54	theme	growout	1391:1397	arg1	wk					1388:1389	4 and 9 wk growout	1380:1397	wk	1388:1389	Relative abundance of C. perfringens estimated by 16S analysis and semiquantitative PCR for netB and Tpel toxin DNA were analyzed by Pearson linear correlation and statistical equivalence analyses with cumulative chick mortality at 4 and 9 wk growout.
35068098	1	55	theme	RNA	282:284	arg1	analysis					246:253	analysis	246:253	analysis of bacterial 16S ribosomal RNA (rRNA) in poultry litter	246:309	The purpose of this study was twofold-first, to determine whether analysis of bacterial 16S ribosomal RNA (rRNA) in poultry litter corroborated standard Clostridium perfringens counts and PCR assay, and second, to find whether a correlation between 16S rRNA analysis and netB or Tpel toxin PCR intensity with chick mortality existed.
35068098	0	56	dep	C.	75:76	arg1	perfringens					78:88	perfringens	78:88	perfringens	78:88	Metagenomic Analysis of 16S Clostridium perfringens Amplicons Corroborates C. perfringens Counts on Select Agar and C. perfringens PCR Analyses of Bacteria in Broiler Farm Litter.
35068098	11	57	theme	chick	2182:2186	arg1	mortality					2188:2196	cumulative percent chick mortality	2163:2196	cumulative percent chick mortality	2163:2196	No correlations were observed between any other genera or species determined by 16S and cumulative percent chick mortality.
35068098	6	58	theme	9	1386:1386	arg1	wk					1388:1389	4 and 9 wk growout	1380:1397	wk	1388:1389	Relative abundance of C. perfringens estimated by 16S analysis and semiquantitative PCR for netB and Tpel toxin DNA were analyzed by Pearson linear correlation and statistical equivalence analyses with cumulative chick mortality at 4 and 9 wk growout.
35068098	0	59	theme	Clostridium	28:38	arg1	Amplicons					52:60	16S Clostridium perfringens Amplicons	24:60	16S Clostridium perfringens Amplicons	24:60	Metagenomic Analysis of 16S Clostridium perfringens Amplicons Corroborates C. perfringens Counts on Select Agar and C. perfringens PCR Analyses of Bacteria in Broiler Farm Litter.
35068098	4	60	theme	perfringens	953:963	arg1	composition					912:922	taxonomic composition	902:922	taxonomic composition	902:922	Obtained sequences were analyzed by QIIME 2 and the Greengenes database for taxonomic composition and relative abundance of C. perfringens in the litter bacterial population.
35068098	4	60	theme	perfringens	953:963	arg1	abundance					937:945	relative abundance	928:945	relative abundance	928:945	Obtained sequences were analyzed by QIIME 2 and the Greengenes database for taxonomic composition and relative abundance of C. perfringens in the litter bacterial population.
35068098	1	61	theme	Tpel	459:462	arg1	intensity					474:482	Tpel toxin PCR intensity	459:482	Tpel toxin PCR intensity	459:482	The purpose of this study was twofold-first, to determine whether analysis of bacterial 16S ribosomal RNA (rRNA) in poultry litter corroborated standard Clostridium perfringens counts and PCR assay, and second, to find whether a correlation between 16S rRNA analysis and netB or Tpel toxin PCR intensity with chick mortality existed.
35068098	6	62	theme	4	1380:1380	arg1	wk					1388:1389	4 and 9 wk growout	1380:1397	wk	1388:1389	Relative abundance of C. perfringens estimated by 16S analysis and semiquantitative PCR for netB and Tpel toxin DNA were analyzed by Pearson linear correlation and statistical equivalence analyses with cumulative chick mortality at 4 and 9 wk growout.
35068098	7	63	theme	C.	1469:1470	arg1	16S					1484:1486	C. perfringens 16S	1469:1486	C. perfringens 16S	1469:1486	When data from all time points were combined, abundance estimates by C. perfringens 16S were statistically equivalent (α = 0.10) to both C. perfringens PCR and C. perfringens counts.
35068098	0	64	dep	Clostridium	28:38	arg1	perfringens					40:50	perfringens	40:50	perfringens	40:50	Metagenomic Analysis of 16S Clostridium perfringens Amplicons Corroborates C. perfringens Counts on Select Agar and C. perfringens PCR Analyses of Bacteria in Broiler Farm Litter.
35068098	1	65	with	correlation	409:419	arg1	mortality					495:503	chick mortality	489:503	chick mortality	489:503	The purpose of this study was twofold-first, to determine whether analysis of bacterial 16S ribosomal RNA (rRNA) in poultry litter corroborated standard Clostridium perfringens counts and PCR assay, and second, to find whether a correlation between 16S rRNA analysis and netB or Tpel toxin PCR intensity with chick mortality existed.
35068098	1	66	theme	PCR	470:472	arg1	intensity					474:482	Tpel toxin PCR intensity	459:482	Tpel toxin PCR intensity	459:482	The purpose of this study was twofold-first, to determine whether analysis of bacterial 16S ribosomal RNA (rRNA) in poultry litter corroborated standard Clostridium perfringens counts and PCR assay, and second, to find whether a correlation between 16S rRNA analysis and netB or Tpel toxin PCR intensity with chick mortality existed.
35068098	9	67	theme	netB	1809:1812	arg1	signal					1814:1819	netB signal	1809:1819	netB signal at 0 wk (r = 0.55) and 4 wk (r = 0.46)	1809:1858	However, correlation analyses revealed a significant linear relationship between netB signal at 0 wk (r = 0.55) and 4 wk (r = 0.46) and cumulative mortality at 9 wk growout (P < 0.05).
35068098	0	68	from	Analyses	135:142	arg1	Agar					107:110	Select Agar	100:110	Select Agar	100:110	Metagenomic Analysis of 16S Clostridium perfringens Amplicons Corroborates C. perfringens Counts on Select Agar and C. perfringens PCR Analyses of Bacteria in Broiler Farm Litter.
35068098	4	69	from	composition	912:922	arg1	population					989:998	the litter bacterial population	968:998	the litter bacterial population	968:998	Obtained sequences were analyzed by QIIME 2 and the Greengenes database for taxonomic composition and relative abundance of C. perfringens in the litter bacterial population.
35068098	9	70	theme	significant	1769:1779	arg1	relationship					1788:1799	a significant linear relationship	1767:1799	a significant linear relationship between netB signal at 0 wk (r = 0.55) and 4 wk (r = 0.46)	1767:1858	However, correlation analyses revealed a significant linear relationship between netB signal at 0 wk (r = 0.55) and 4 wk (r = 0.46) and cumulative mortality at 9 wk growout (P < 0.05).
35068098	9	71	theme	cumulative	1864:1873	arg1	mortality					1875:1883	cumulative mortality	1864:1883	cumulative mortality at 9 wk growout (P < 0.05)	1864:1910	However, correlation analyses revealed a significant linear relationship between netB signal at 0 wk (r = 0.55) and 4 wk (r = 0.46) and cumulative mortality at 9 wk growout (P < 0.05).
35068098	6	72	theme	toxin	1254:1258	arg1	DNA					1260:1262	Tpel toxin DNA	1249:1262	Tpel toxin DNA	1249:1262	Relative abundance of C. perfringens estimated by 16S analysis and semiquantitative PCR for netB and Tpel toxin DNA were analyzed by Pearson linear correlation and statistical equivalence analyses with cumulative chick mortality at 4 and 9 wk growout.
35068098	10	73	theme	9	2033:2033	arg1	wk					2035:2036	4 and 9 wk growout	2027:2044	wk	2035:2036	Similarly, abundance of Tpel at 0 and 2 wk showed a linear relationship with cumulative percent mortality at both 4 and 9 wk growout (0.44 ≤ r ≤ 0.54, P < 0.05).
35068098	10	73	theme	9	2033:2033	arg1	0.54					2058:2061	0.44 ≤ r ≤ 0.54	2047:2061	0.44 ≤ r ≤ 0.54	2047:2061	Similarly, abundance of Tpel at 0 and 2 wk showed a linear relationship with cumulative percent mortality at both 4 and 9 wk growout (0.44 ≤ r ≤ 0.54, P < 0.05).
35068098	9	74	from	wk	1890:1891	arg1	relationship					1788:1799	a significant linear relationship	1767:1799	a significant linear relationship between netB signal at 0 wk (r = 0.55) and 4 wk (r = 0.46)	1767:1858	However, correlation analyses revealed a significant linear relationship between netB signal at 0 wk (r = 0.55) and 4 wk (r = 0.46) and cumulative mortality at 9 wk growout (P < 0.05).
35068098	9	74	from	wk	1890:1891	arg1	mortality					1875:1883	cumulative mortality	1864:1883	cumulative mortality at 9 wk growout (P < 0.05)	1864:1910	However, correlation analyses revealed a significant linear relationship between netB signal at 0 wk (r = 0.55) and 4 wk (r = 0.46) and cumulative mortality at 9 wk growout (P < 0.05).
35068098	10	75	theme	Tpel	1937:1940	arg1	abundance					1924:1932	abundance	1924:1932	abundance of Tpel at 0 and 2 wk	1924:1954	Similarly, abundance of Tpel at 0 and 2 wk showed a linear relationship with cumulative percent mortality at both 4 and 9 wk growout (0.44 ≤ r ≤ 0.54, P < 0.05).
35068098	7	76	dep	C.	1469:1470	arg1	perfringens					1472:1482	perfringens	1472:1482	perfringens	1472:1482	When data from all time points were combined, abundance estimates by C. perfringens 16S were statistically equivalent (α = 0.10) to both C. perfringens PCR and C. perfringens counts.
35068098	7	77	theme	time	1419:1422	arg1	points					1424:1429	all time points	1415:1429	all time points	1415:1429	When data from all time points were combined, abundance estimates by C. perfringens 16S were statistically equivalent (α = 0.10) to both C. perfringens PCR and C. perfringens counts.
35068098	9	78	theme	r	1830:1830	arg1	0.55					1834:1837	r = 0.55	1830:1837	r = 0.55	1830:1837	However, correlation analyses revealed a significant linear relationship between netB signal at 0 wk (r = 0.55) and 4 wk (r = 0.46) and cumulative mortality at 9 wk growout (P < 0.05).
35068098	9	78	theme	r	1830:1830	arg1	wk					1826:1827	0 wk	1824:1827	0 wk (r = 0.55)	1824:1838	However, correlation analyses revealed a significant linear relationship between netB signal at 0 wk (r = 0.55) and 4 wk (r = 0.46) and cumulative mortality at 9 wk growout (P < 0.05).
35068098	1	79	from	RNA	282:284	arg1	litter					304:309	poultry litter	296:309	poultry litter	296:309	The purpose of this study was twofold-first, to determine whether analysis of bacterial 16S ribosomal RNA (rRNA) in poultry litter corroborated standard Clostridium perfringens counts and PCR assay, and second, to find whether a correlation between 16S rRNA analysis and netB or Tpel toxin PCR intensity with chick mortality existed.
35068098	1	80	theme	16S	429:431	arg1	rRNA					433:436	16S rRNA	429:436	16S rRNA analysis	429:445	The purpose of this study was twofold-first, to determine whether analysis of bacterial 16S ribosomal RNA (rRNA) in poultry litter corroborated standard Clostridium perfringens counts and PCR assay, and second, to find whether a correlation between 16S rRNA analysis and netB or Tpel toxin PCR intensity with chick mortality existed.
35068098	0	81	theme	Amplicons	52:60	arg1	Analysis					12:19	Metagenomic Analysis	0:19	Metagenomic Analysis of 16S Clostridium perfringens Amplicons	0:60	Metagenomic Analysis of 16S Clostridium perfringens Amplicons Corroborates C. perfringens Counts on Select Agar and C. perfringens PCR Analyses of Bacteria in Broiler Farm Litter.
35068098	6	82	theme	semiquantitative	1215:1230	arg1	PCR					1232:1234	semiquantitative PCR	1215:1234	semiquantitative PCR	1215:1234	Relative abundance of C. perfringens estimated by 16S analysis and semiquantitative PCR for netB and Tpel toxin DNA were analyzed by Pearson linear correlation and statistical equivalence analyses with cumulative chick mortality at 4 and 9 wk growout.
35068098	6	83	with	correlation	1296:1306	arg1	mortality					1367:1375	cumulative chick mortality	1350:1375	cumulative chick mortality	1350:1375	Relative abundance of C. perfringens estimated by 16S analysis and semiquantitative PCR for netB and Tpel toxin DNA were analyzed by Pearson linear correlation and statistical equivalence analyses with cumulative chick mortality at 4 and 9 wk growout.
35068098	9	84	theme	P	1902:1902	arg1	<					1904:1904	P < 0.05	1902:1909	P < 0.05	1902:1909	However, correlation analyses revealed a significant linear relationship between netB signal at 0 wk (r = 0.55) and 4 wk (r = 0.46) and cumulative mortality at 9 wk growout (P < 0.05).
35068098	9	84	theme	P	1902:1902	arg1	wk					1890:1891	9 wk growout	1888:1899	9 wk growout (P < 0.05)	1888:1910	However, correlation analyses revealed a significant linear relationship between netB signal at 0 wk (r = 0.55) and 4 wk (r = 0.46) and cumulative mortality at 9 wk growout (P < 0.05).
35068098	0	85	theme	PCR	131:133	arg1	Analyses					135:142	C. perfringens PCR Analyses	116:142	C. perfringens PCR Analyses of Bacteria	116:154	Metagenomic Analysis of 16S Clostridium perfringens Amplicons Corroborates C. perfringens Counts on Select Agar and C. perfringens PCR Analyses of Bacteria in Broiler Farm Litter.
35068098	7	86	from	points	1424:1429	arg1	data					1405:1408	data	1405:1408	data from all time points	1405:1429	When data from all time points were combined, abundance estimates by C. perfringens 16S were statistically equivalent (α = 0.10) to both C. perfringens PCR and C. perfringens counts.
35068098	10	87	theme	linear	1965:1970	arg1	relationship					1972:1983	a linear relationship	1963:1983	a linear relationship with cumulative percent mortality at both 4 and 9 wk growout (0.44 ≤ r ≤ 0.54, P < 0.05)	1963:2072	Similarly, abundance of Tpel at 0 and 2 wk showed a linear relationship with cumulative percent mortality at both 4 and 9 wk growout (0.44 ≤ r ≤ 0.54, P < 0.05).
35068098	6	88	theme	linear	1289:1294	arg1	correlation					1296:1306	Pearson linear correlation	1281:1306	Pearson linear correlation	1281:1306	Relative abundance of C. perfringens estimated by 16S analysis and semiquantitative PCR for netB and Tpel toxin DNA were analyzed by Pearson linear correlation and statistical equivalence analyses with cumulative chick mortality at 4 and 9 wk growout.
35068098	8	89	theme	percent	1682:1688	arg1	mortality					1696:1704	cumulative percent chick mortality	1671:1704	cumulative percent chick mortality	1671:1704	Yet, no correlations were observed between any estimate of C. perfringens abundance and cumulative percent chick mortality at 4 or 9 wk growout.
35068098	1	90	theme	study	200:204	arg1	twofold-first					210:222	twofold-first	210:222	twofold-first	210:222	The purpose of this study was twofold-first, to determine whether analysis of bacterial 16S ribosomal RNA (rRNA) in poultry litter corroborated standard Clostridium perfringens counts and PCR assay, and second, to find whether a correlation between 16S rRNA analysis and netB or Tpel toxin PCR intensity with chick mortality existed.
35068098	1	90	theme	study	200:204	arg1	purpose					184:190	The purpose	180:190	The purpose of this study	180:204	The purpose of this study was twofold-first, to determine whether analysis of bacterial 16S ribosomal RNA (rRNA) in poultry litter corroborated standard Clostridium perfringens counts and PCR assay, and second, to find whether a correlation between 16S rRNA analysis and netB or Tpel toxin PCR intensity with chick mortality existed.
35068098	6	91	theme	16S	1198:1200	arg1	analysis					1202:1209	16S analysis	1198:1209	16S analysis	1198:1209	Relative abundance of C. perfringens estimated by 16S analysis and semiquantitative PCR for netB and Tpel toxin DNA were analyzed by Pearson linear correlation and statistical equivalence analyses with cumulative chick mortality at 4 and 9 wk growout.
35068098	1	92	theme	poultry	296:302	arg1	litter					304:309	poultry litter	296:309	poultry litter	296:309	The purpose of this study was twofold-first, to determine whether analysis of bacterial 16S ribosomal RNA (rRNA) in poultry litter corroborated standard Clostridium perfringens counts and PCR assay, and second, to find whether a correlation between 16S rRNA analysis and netB or Tpel toxin PCR intensity with chick mortality existed.
35068098	7	93	dep	C.	1537:1538	arg1	perfringens					1540:1550	perfringens	1540:1550	perfringens	1540:1550	When data from all time points were combined, abundance estimates by C. perfringens 16S were statistically equivalent (α = 0.10) to both C. perfringens PCR and C. perfringens counts.
35068098	4	94	theme	Obtained	826:833	arg1	sequences					835:843	Obtained sequences	826:843	Obtained sequences	826:843	Obtained sequences were analyzed by QIIME 2 and the Greengenes database for taxonomic composition and relative abundance of C. perfringens in the litter bacterial population.
35068098	10	95	theme	percent	2001:2007	arg1	mortality					2009:2017	cumulative percent mortality	1990:2017	cumulative percent mortality at both 4 and 9 wk growout (0.44 ≤ r ≤ 0.54, P < 0.05)	1990:2072	Similarly, abundance of Tpel at 0 and 2 wk showed a linear relationship with cumulative percent mortality at both 4 and 9 wk growout (0.44 ≤ r ≤ 0.54, P < 0.05).
35068098	10	96	from	wk	2035:2036	arg1	mortality					2009:2017	cumulative percent mortality	1990:2017	cumulative percent mortality at both 4 and 9 wk growout (0.44 ≤ r ≤ 0.54, P < 0.05)	1990:2072	Similarly, abundance of Tpel at 0 and 2 wk showed a linear relationship with cumulative percent mortality at both 4 and 9 wk growout (0.44 ≤ r ≤ 0.54, P < 0.05).
35068098	0	97	theme	Farm	167:170	arg1	Litter					172:177	Broiler Farm Litter	159:177	Broiler Farm Litter	159:177	Metagenomic Analysis of 16S Clostridium perfringens Amplicons Corroborates C. perfringens Counts on Select Agar and C. perfringens PCR Analyses of Bacteria in Broiler Farm Litter.
35068098	8	98	theme	4	1709:1709	arg1	wk					1716:1717	4 or 9 wk growout	1709:1725	wk	1716:1717	Yet, no correlations were observed between any estimate of C. perfringens abundance and cumulative percent chick mortality at 4 or 9 wk growout.
35068098	2	99	theme	time	523:526	arg1	points					528:533	three time points	517:533	three time points of growout (0, 2, and 4 wk)	517:561	At three time points of growout (0, 2, and 4 wk) litter samples were collected from 23 broiler houses representing eight farms during a coccidiosis vaccine control program.
35068098	6	100	theme	C.	1170:1171	arg1	perfringens					1173:1183	C. perfringens	1170:1183	C. perfringens estimated by 16S analysis and semiquantitative PCR for netB and Tpel toxin DNA	1170:1262	Relative abundance of C. perfringens estimated by 16S analysis and semiquantitative PCR for netB and Tpel toxin DNA were analyzed by Pearson linear correlation and statistical equivalence analyses with cumulative chick mortality at 4 and 9 wk growout.
35068098	2	101	theme	vaccine	662:668	arg1	program					678:684	a coccidiosis vaccine control program	648:684	a coccidiosis vaccine control program	648:684	At three time points of growout (0, 2, and 4 wk) litter samples were collected from 23 broiler houses representing eight farms during a coccidiosis vaccine control program.
35068098	3	102	theme	microbiota	733:742	arg1	determination					744:756	microbiota determination	733:756	microbiota determination	733:756	DNA extracted from these samples was used for microbiota determination by sequencing the hypervariable V3-V4 region of bacterial 16s rRNA.
35068098	8	103	theme	9	1714:1714	arg1	wk					1716:1717	4 or 9 wk growout	1709:1725	wk	1716:1717	Yet, no correlations were observed between any estimate of C. perfringens abundance and cumulative percent chick mortality at 4 or 9 wk growout.
35068098	3	104	theme	V3-V4	790:794	arg1	region					796:801	the hypervariable V3-V4 region	772:801	the hypervariable V3-V4 region of bacterial 16s rRNA	772:823	DNA extracted from these samples was used for microbiota determination by sequencing the hypervariable V3-V4 region of bacterial 16s rRNA.
35068098	4	105	theme	taxonomic	902:910	arg1	composition					912:922	taxonomic composition	902:922	taxonomic composition	902:922	Obtained sequences were analyzed by QIIME 2 and the Greengenes database for taxonomic composition and relative abundance of C. perfringens in the litter bacterial population.
35068098	3	106	used	used	724:727	arg2	DNA					687:689	DNA	687:689	DNA extracted from these samples	687:718	DNA extracted from these samples was used for microbiota determination by sequencing the hypervariable V3-V4 region of bacterial 16s rRNA.
35068098	10	107	theme	4	2027:2027	arg1	wk					2035:2036	4 and 9 wk growout	2027:2044	wk	2035:2036	Similarly, abundance of Tpel at 0 and 2 wk showed a linear relationship with cumulative percent mortality at both 4 and 9 wk growout (0.44 ≤ r ≤ 0.54, P < 0.05).
35068098	10	107	theme	4	2027:2027	arg1	0.54					2058:2061	0.44 ≤ r ≤ 0.54	2047:2061	0.44 ≤ r ≤ 0.54	2047:2061	Similarly, abundance of Tpel at 0 and 2 wk showed a linear relationship with cumulative percent mortality at both 4 and 9 wk growout (0.44 ≤ r ≤ 0.54, P < 0.05).
35068098	6	108	theme	chick	1361:1365	arg1	mortality					1367:1375	cumulative chick mortality	1350:1375	cumulative chick mortality	1350:1375	Relative abundance of C. perfringens estimated by 16S analysis and semiquantitative PCR for netB and Tpel toxin DNA were analyzed by Pearson linear correlation and statistical equivalence analyses with cumulative chick mortality at 4 and 9 wk growout.
35068098	6	109	with	analyses	1336:1343	arg1	mortality					1367:1375	cumulative chick mortality	1350:1375	cumulative chick mortality	1350:1375	Relative abundance of C. perfringens estimated by 16S analysis and semiquantitative PCR for netB and Tpel toxin DNA were analyzed by Pearson linear correlation and statistical equivalence analyses with cumulative chick mortality at 4 and 9 wk growout.
35068098	10	110	dep	wk	2035:2036	arg1	both					2022:2025	both	2022:2025	both	2022:2025	Similarly, abundance of Tpel at 0 and 2 wk showed a linear relationship with cumulative percent mortality at both 4 and 9 wk growout (0.44 ≤ r ≤ 0.54, P < 0.05).
35068098	11	111	theme	other	2117:2121	arg1	genera					2123:2128	any other genera	2113:2128	any other genera	2113:2128	No correlations were observed between any other genera or species determined by 16S and cumulative percent chick mortality.
35068098	1	112	theme	Clostridium	333:343	arg1	counts					357:362	standard Clostridium perfringens counts	324:362	standard Clostridium perfringens counts	324:362	The purpose of this study was twofold-first, to determine whether analysis of bacterial 16S ribosomal RNA (rRNA) in poultry litter corroborated standard Clostridium perfringens counts and PCR assay, and second, to find whether a correlation between 16S rRNA analysis and netB or Tpel toxin PCR intensity with chick mortality existed.
35068098	9	113	theme	growout	1893:1899	arg1	<					1904:1904	P < 0.05	1902:1909	P < 0.05	1902:1909	However, correlation analyses revealed a significant linear relationship between netB signal at 0 wk (r = 0.55) and 4 wk (r = 0.46) and cumulative mortality at 9 wk growout (P < 0.05).
35068098	9	113	theme	growout	1893:1899	arg1	wk					1890:1891	9 wk growout	1888:1899	9 wk growout (P < 0.05)	1888:1910	However, correlation analyses revealed a significant linear relationship between netB signal at 0 wk (r = 0.55) and 4 wk (r = 0.46) and cumulative mortality at 9 wk growout (P < 0.05).
35068098	0	114	dep	C.	116:117	arg1	perfringens					119:129	perfringens	119:129	perfringens	119:129	Metagenomic Analysis of 16S Clostridium perfringens Amplicons Corroborates C. perfringens Counts on Select Agar and C. perfringens PCR Analyses of Bacteria in Broiler Farm Litter.
35068098	3	115	theme	16s	816:818	arg1	rRNA					820:823	bacterial 16s rRNA	806:823	bacterial 16s rRNA	806:823	DNA extracted from these samples was used for microbiota determination by sequencing the hypervariable V3-V4 region of bacterial 16s rRNA.
35068098	2	116	theme	litter	563:568	arg1	samples					570:576	litter samples	563:576	litter samples	563:576	At three time points of growout (0, 2, and 4 wk) litter samples were collected from 23 broiler houses representing eight farms during a coccidiosis vaccine control program.
35068098	5	117	from	counts	1025:1030	arg1	PCR					1068:1070	semiquantitative PCR	1051:1070	semiquantitative PCR	1051:1070	Clostridium perfringens counts on select agar and semiquantitative PCR for C. perfringens were compared with 16S analysis for equivalence testing.
35068098	5	117	from	counts	1025:1030	arg1	agar					1042:1045	select agar	1035:1045	select agar	1035:1045	Clostridium perfringens counts on select agar and semiquantitative PCR for C. perfringens were compared with 16S analysis for equivalence testing.
35068098	5	118	theme	semiquantitative	1051:1066	arg1	PCR					1068:1070	semiquantitative PCR	1051:1070	semiquantitative PCR	1051:1070	Clostridium perfringens counts on select agar and semiquantitative PCR for C. perfringens were compared with 16S analysis for equivalence testing.
35068098	2	119	theme	broiler	601:607	arg1	houses					609:614	23 broiler houses	598:614	23 broiler houses representing eight farms during a coccidiosis vaccine control program	598:684	At three time points of growout (0, 2, and 4 wk) litter samples were collected from 23 broiler houses representing eight farms during a coccidiosis vaccine control program.
35068098	0	120	theme	C.	116:117	arg1	Analyses					135:142	C. perfringens PCR Analyses	116:142	C. perfringens PCR Analyses of Bacteria	116:154	Metagenomic Analysis of 16S Clostridium perfringens Amplicons Corroborates C. perfringens Counts on Select Agar and C. perfringens PCR Analyses of Bacteria in Broiler Farm Litter.
35068098	1	121	theme	PCR	368:370	arg1	assay					372:376	PCR assay	368:376	PCR assay	368:376	The purpose of this study was twofold-first, to determine whether analysis of bacterial 16S ribosomal RNA (rRNA) in poultry litter corroborated standard Clostridium perfringens counts and PCR assay, and second, to find whether a correlation between 16S rRNA analysis and netB or Tpel toxin PCR intensity with chick mortality existed.
35068098	8	122	dep	C.	1642:1643	arg1	perfringens					1645:1655	perfringens	1645:1655	perfringens	1645:1655	Yet, no correlations were observed between any estimate of C. perfringens abundance and cumulative percent chick mortality at 4 or 9 wk growout.
35068098	0	123	theme	16S	24:26	arg1	Amplicons					52:60	16S Clostridium perfringens Amplicons	24:60	16S Clostridium perfringens Amplicons	24:60	Metagenomic Analysis of 16S Clostridium perfringens Amplicons Corroborates C. perfringens Counts on Select Agar and C. perfringens PCR Analyses of Bacteria in Broiler Farm Litter.
35068098	1	124	theme	ribosomal	272:280	arg1	RNA					282:284	bacterial 16S ribosomal RNA	258:284	bacterial 16S ribosomal RNA (rRNA) in poultry litter	258:309	The purpose of this study was twofold-first, to determine whether analysis of bacterial 16S ribosomal RNA (rRNA) in poultry litter corroborated standard Clostridium perfringens counts and PCR assay, and second, to find whether a correlation between 16S rRNA analysis and netB or Tpel toxin PCR intensity with chick mortality existed.
35068098	1	124	theme	ribosomal	272:280	arg1	rRNA					287:290	rRNA	287:290	rRNA	287:290	The purpose of this study was twofold-first, to determine whether analysis of bacterial 16S ribosomal RNA (rRNA) in poultry litter corroborated standard Clostridium perfringens counts and PCR assay, and second, to find whether a correlation between 16S rRNA analysis and netB or Tpel toxin PCR intensity with chick mortality existed.
35068098	9	125	theme	linear	1781:1786	arg1	relationship					1788:1799	a significant linear relationship	1767:1799	a significant linear relationship between netB signal at 0 wk (r = 0.55) and 4 wk (r = 0.46)	1767:1858	However, correlation analyses revealed a significant linear relationship between netB signal at 0 wk (r = 0.55) and 4 wk (r = 0.46) and cumulative mortality at 9 wk growout (P < 0.05).
35068098	4	126	theme	litter	972:977	arg1	population					989:998	the litter bacterial population	968:998	the litter bacterial population	968:998	Obtained sequences were analyzed by QIIME 2 and the Greengenes database for taxonomic composition and relative abundance of C. perfringens in the litter bacterial population.
35068098	1	127	from	litter	304:309	arg1	analysis					246:253	analysis	246:253	analysis of bacterial 16S ribosomal RNA (rRNA) in poultry litter	246:309	The purpose of this study was twofold-first, to determine whether analysis of bacterial 16S ribosomal RNA (rRNA) in poultry litter corroborated standard Clostridium perfringens counts and PCR assay, and second, to find whether a correlation between 16S rRNA analysis and netB or Tpel toxin PCR intensity with chick mortality existed.
35068098	5	128	theme	16S	1110:1112	arg1	analysis					1114:1121	16S analysis	1110:1121	16S analysis for equivalence testing	1110:1145	Clostridium perfringens counts on select agar and semiquantitative PCR for C. perfringens were compared with 16S analysis for equivalence testing.
35068098	4	129	theme	C.	950:951	arg1	perfringens					953:963	C. perfringens	950:963	C. perfringens	950:963	Obtained sequences were analyzed by QIIME 2 and the Greengenes database for taxonomic composition and relative abundance of C. perfringens in the litter bacterial population.
35068098	10	130	from	wk	1953:1954	arg1	abundance					1924:1932	abundance	1924:1932	abundance of Tpel at 0 and 2 wk	1924:1954	Similarly, abundance of Tpel at 0 and 2 wk showed a linear relationship with cumulative percent mortality at both 4 and 9 wk growout (0.44 ≤ r ≤ 0.54, P < 0.05).
35068098	1	131	theme	toxin	464:468	arg1	intensity					474:482	Tpel toxin PCR intensity	459:482	Tpel toxin PCR intensity	459:482	The purpose of this study was twofold-first, to determine whether analysis of bacterial 16S ribosomal RNA (rRNA) in poultry litter corroborated standard Clostridium perfringens counts and PCR assay, and second, to find whether a correlation between 16S rRNA analysis and netB or Tpel toxin PCR intensity with chick mortality existed.
35068098	4	132	from	abundance	937:945	arg1	population					989:998	the litter bacterial population	968:998	the litter bacterial population	968:998	Obtained sequences were analyzed by QIIME 2 and the Greengenes database for taxonomic composition and relative abundance of C. perfringens in the litter bacterial population.
35068098	8	133	dep	abundance	1657:1665	arg1	estimate					1630:1637	estimate	1630:1637	estimate	1630:1637	Yet, no correlations were observed between any estimate of C. perfringens abundance and cumulative percent chick mortality at 4 or 9 wk growout.
35068098	6	134	theme	Pearson	1281:1287	arg1	correlation					1296:1306	Pearson linear correlation	1281:1306	Pearson linear correlation	1281:1306	Relative abundance of C. perfringens estimated by 16S analysis and semiquantitative PCR for netB and Tpel toxin DNA were analyzed by Pearson linear correlation and statistical equivalence analyses with cumulative chick mortality at 4 and 9 wk growout.
35068098	1	135	with	intensity	474:482	arg1	mortality					495:503	chick mortality	489:503	chick mortality	489:503	The purpose of this study was twofold-first, to determine whether analysis of bacterial 16S ribosomal RNA (rRNA) in poultry litter corroborated standard Clostridium perfringens counts and PCR assay, and second, to find whether a correlation between 16S rRNA analysis and netB or Tpel toxin PCR intensity with chick mortality existed.
35068098	11	136	theme	cumulative	2163:2172	arg1	mortality					2188:2196	cumulative percent chick mortality	2163:2196	cumulative percent chick mortality	2163:2196	No correlations were observed between any other genera or species determined by 16S and cumulative percent chick mortality.
35068098	1	137	theme	chick	489:493	arg1	mortality					495:503	chick mortality	489:503	chick mortality	489:503	The purpose of this study was twofold-first, to determine whether analysis of bacterial 16S ribosomal RNA (rRNA) in poultry litter corroborated standard Clostridium perfringens counts and PCR assay, and second, to find whether a correlation between 16S rRNA analysis and netB or Tpel toxin PCR intensity with chick mortality existed.
34740036	6	0	theme	bacterial	1057:1065	arg1	growth					1067:1072	bacterial growth	1057:1072	bacterial growth	1057:1072	Furthermore, POTs show negligible effect on bacterial growth in vitro, healthy mice and their microbiota composition under homeostatic conditions.
34740036	3	1	from	loss	504:507	arg1	caecum					635:640	caecum	635:640	caecum	635:640	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	3	1	from	loss	504:507	arg1	colon					655:659	proximal colon	646:659	proximal colon	646:659	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	2	2	theme	colitis	317:323	arg1	model					325:329	a dextran sulfate sodium (DSS)-induced colitis model	278:329	a dextran sulfate sodium (DSS)-induced colitis model	278:329	In a dextran sulfate sodium (DSS)-induced colitis model, symptom remission of C57BL/6 J mice with colitis is achieved by orally treated with POT complexes.
34740036	4	3	theme	magnitude	787:795	arg1	order					778:782	one order	774:782	one order of magnitude	774:795	Bacterial population analysis reveals that these Enterobacteriaceae population in the caecal content decline by one order of magnitude after administration of POT complexes.
34740036	3	4	theme	5	489:489	arg1	%					490:490	%	490:490	%	490:490	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	1	5	theme	Enterobacteriaceae	248:265	arg1	family					267:272	the Enterobacteriaceae family	244:272	the Enterobacteriaceae family	244:272	Here we show that Preyssler-type polyoxotungstates (Preyssler-type POTs, [NaP5W30O110]-14) complexed with peptides can prevent the dysbiotic expansion of anaerobic bacteria of the Enterobacteriaceae family.
34740036	7	6	theme	POT	1180:1182	arg1	complexes					1184:1192	Rationally designed POT complexes	1160:1192	Rationally designed POT complexes	1160:1192	Rationally designed POT complexes will provide distinctive approach to improve enteric bacteria dysbiosis-associated gut inflammation by balancing bacterial communities.
34740036	5	7	theme	immune	905:910	arg1	system					912:917	the host immune system	896:917	the host immune system	896:917	POT complexes exert anti-inflammatory effects indirectly on the host immune system by inhibition of malignant expansion of anaerobic Enterobacteriaceae during gut inflammation.
34740036	5	8	theme	host	900:903	arg1	system					912:917	the host immune system	896:917	the host immune system	896:917	POT complexes exert anti-inflammatory effects indirectly on the host immune system by inhibition of malignant expansion of anaerobic Enterobacteriaceae during gut inflammation.
34740036	1	9	theme	family	267:272	arg1	bacteria					232:239	anaerobic bacteria	222:239	anaerobic bacteria of the Enterobacteriaceae family	222:272	Here we show that Preyssler-type polyoxotungstates (Preyssler-type POTs, [NaP5W30O110]-14) complexed with peptides can prevent the dysbiotic expansion of anaerobic bacteria of the Enterobacteriaceae family.
34740036	7	10	theme	dysbiosis-associated	1256:1275	arg1	inflammation					1281:1292	enteric bacteria dysbiosis-associated gut inflammation	1239:1292	enteric bacteria dysbiosis-associated gut inflammation	1239:1292	Rationally designed POT complexes will provide distinctive approach to improve enteric bacteria dysbiosis-associated gut inflammation by balancing bacterial communities.
34740036	6	11	theme	microbiota	1107:1116	arg1	composition					1118:1128	their microbiota composition	1101:1128	their microbiota composition	1101:1128	Furthermore, POTs show negligible effect on bacterial growth in vitro, healthy mice and their microbiota composition under homeostatic conditions.
34740036	6	12	from	effect	1047:1052	arg1	growth					1067:1072	bacterial growth	1057:1072	bacterial growth	1057:1072	Furthermore, POTs show negligible effect on bacterial growth in vitro, healthy mice and their microbiota composition under homeostatic conditions.
34740036	6	12	from	effect	1047:1052	arg1	mice					1092:1095	vitro, healthy mice	1077:1095	mice	1092:1095	Furthermore, POTs show negligible effect on bacterial growth in vitro, healthy mice and their microbiota composition under homeostatic conditions.
34740036	4	13	theme	population	672:681	arg1	analysis					683:690	Bacterial population analysis	662:690	Bacterial population analysis	662:690	Bacterial population analysis reveals that these Enterobacteriaceae population in the caecal content decline by one order of magnitude after administration of POT complexes.
34740036	3	14	theme	proinflammatory	532:546	arg1	markers					548:554	proinflammatory markers	532:554	proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1)	532:626	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	7	15	theme	enteric	1239:1245	arg1	bacteria					1247:1254	enteric bacteria	1239:1254	enteric bacteria dysbiosis-associated gut inflammation	1239:1292	Rationally designed POT complexes will provide distinctive approach to improve enteric bacteria dysbiosis-associated gut inflammation by balancing bacterial communities.
34740036	3	16	theme	daily	443:447	arg1	administration					449:462	Ten days of daily administration	431:462	Ten days of daily administration of POT complexes	431:479	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	3	17	theme	weight	497:502	arg1	loss					504:507	5% body weight loss	489:507	5% body weight loss	489:507	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	5	18	theme	anti-inflammatory	856:872	arg1	effects					874:880	anti-inflammatory effects	856:880	anti-inflammatory effects	856:880	POT complexes exert anti-inflammatory effects indirectly on the host immune system by inhibition of malignant expansion of anaerobic Enterobacteriaceae during gut inflammation.
34740036	3	19	dep	markers	548:554	arg1	reduction					584:592	73% reduction	580:592	73% reduction for Tnf	580:600	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	3	19	dep	markers	548:554	arg1	reduction					607:615	91% reduction	603:615	91% reduction for Cxcl1	603:625	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	3	19	dep	markers	548:554	arg1	reduction					561:569	77% reduction	557:569	77% reduction for Il6	557:577	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	7	20	theme	designed	1171:1178	arg1	complexes					1184:1192	Rationally designed POT complexes	1160:1192	Rationally designed POT complexes	1160:1192	Rationally designed POT complexes will provide distinctive approach to improve enteric bacteria dysbiosis-associated gut inflammation by balancing bacterial communities.
34740036	0	21	from	polyoxotungstates	37:53	arg1	gut					63:65	mice gut	58:65	mice gut	58:65	Amelioration of enteric dysbiosis by polyoxotungstates in mice gut.
34740036	6	22	theme	negligible	1036:1045	arg1	effect					1047:1052	negligible effect	1036:1052	negligible effect on bacterial growth in vitro, healthy mice	1036:1095	Furthermore, POTs show negligible effect on bacterial growth in vitro, healthy mice and their microbiota composition under homeostatic conditions.
34740036	5	23	theme	Enterobacteriaceae	969:986	arg1	expansion					946:954	malignant expansion	936:954	malignant expansion of anaerobic Enterobacteriaceae during gut inflammation	936:1010	POT complexes exert anti-inflammatory effects indirectly on the host immune system by inhibition of malignant expansion of anaerobic Enterobacteriaceae during gut inflammation.
34740036	5	24	theme	POT	836:838	arg1	complexes					840:848	POT complexes	836:848	POT complexes	836:848	POT complexes exert anti-inflammatory effects indirectly on the host immune system by inhibition of malignant expansion of anaerobic Enterobacteriaceae during gut inflammation.
34740036	4	25	theme	Bacterial	662:670	arg1	analysis					683:690	Bacterial population analysis	662:690	Bacterial population analysis	662:690	Bacterial population analysis reveals that these Enterobacteriaceae population in the caecal content decline by one order of magnitude after administration of POT complexes.
34740036	7	26	theme	gut	1277:1279	arg1	inflammation					1281:1292	enteric bacteria dysbiosis-associated gut inflammation	1239:1292	enteric bacteria dysbiosis-associated gut inflammation	1239:1292	Rationally designed POT complexes will provide distinctive approach to improve enteric bacteria dysbiosis-associated gut inflammation by balancing bacterial communities.
34740036	3	27	theme	91	603:604	arg1	%					605:605	%	605:605	%	605:605	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	2	28	theme	mice	363:366	arg1	remission					340:348	symptom remission	332:348	symptom remission of C57BL/6 J mice with colitis	332:379	In a dextran sulfate sodium (DSS)-induced colitis model, symptom remission of C57BL/6 J mice with colitis is achieved by orally treated with POT complexes.
34740036	0	29	theme	dysbiosis	24:32	arg1	Amelioration					0:11	Amelioration	0:11	Amelioration of enteric dysbiosis by polyoxotungstates in mice gut.	0:66	Amelioration of enteric dysbiosis by polyoxotungstates in mice gut.
34740036	1	30	theme	Preyssler-type	86:99	arg1	[NaP5W30O110					141:152	Preyssler-type POTs, [NaP5W30O110]-14	120:156	[NaP5W30O110	141:152	Here we show that Preyssler-type polyoxotungstates (Preyssler-type POTs, [NaP5W30O110]-14) complexed with peptides can prevent the dysbiotic expansion of anaerobic bacteria of the Enterobacteriaceae family.
34740036	1	30	theme	Preyssler-type	86:99	arg1	polyoxotungstates					101:117	Preyssler-type polyoxotungstates	86:117	Preyssler-type polyoxotungstates (Preyssler-type POTs, [NaP5W30O110]-14) complexed with peptides	86:181	Here we show that Preyssler-type polyoxotungstates (Preyssler-type POTs, [NaP5W30O110]-14) complexed with peptides can prevent the dysbiotic expansion of anaerobic bacteria of the Enterobacteriaceae family.
34740036	5	31	theme	anaerobic	959:967	arg1	Enterobacteriaceae					969:986	anaerobic Enterobacteriaceae	959:986	anaerobic Enterobacteriaceae	959:986	POT complexes exert anti-inflammatory effects indirectly on the host immune system by inhibition of malignant expansion of anaerobic Enterobacteriaceae during gut inflammation.
34740036	3	32	theme	of	440:441	arg1	administration					449:462	Ten days of daily administration	431:462	Ten days of daily administration of POT complexes	431:479	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	0	33	theme	enteric	16:22	arg1	dysbiosis					24:32	enteric dysbiosis	16:32	enteric dysbiosis	16:32	Amelioration of enteric dysbiosis by polyoxotungstates in mice gut.
34740036	3	34	theme	proximal	646:653	arg1	colon					655:659	proximal colon	646:659	proximal colon	646:659	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	6	35	theme	homeostatic	1136:1146	arg1	conditions					1148:1157	homeostatic conditions	1136:1157	homeostatic conditions	1136:1157	Furthermore, POTs show negligible effect on bacterial growth in vitro, healthy mice and their microbiota composition under homeostatic conditions.
34740036	2	36	theme	symptom	332:338	arg1	remission					340:348	symptom remission	332:348	symptom remission of C57BL/6 J mice with colitis	332:379	In a dextran sulfate sodium (DSS)-induced colitis model, symptom remission of C57BL/6 J mice with colitis is achieved by orally treated with POT complexes.
34740036	6	37	from	composition	1118:1128	arg1	growth					1067:1072	bacterial growth	1057:1072	bacterial growth	1057:1072	Furthermore, POTs show negligible effect on bacterial growth in vitro, healthy mice and their microbiota composition under homeostatic conditions.
34740036	6	37	from	composition	1118:1128	arg1	mice					1092:1095	vitro, healthy mice	1077:1095	mice	1092:1095	Furthermore, POTs show negligible effect on bacterial growth in vitro, healthy mice and their microbiota composition under homeostatic conditions.
34740036	3	38	theme	%	490:490	arg1	loss					504:507	5% body weight loss	489:507	5% body weight loss	489:507	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	3	39	theme	complexes	471:479	arg1	administration					449:462	Ten days of daily administration	431:462	Ten days of daily administration of POT complexes	431:479	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	6	40	theme	healthy	1084:1090	arg1	mice					1092:1095	vitro, healthy mice	1077:1095	mice	1092:1095	Furthermore, POTs show negligible effect on bacterial growth in vitro, healthy mice and their microbiota composition under homeostatic conditions.
34740036	4	41	theme	caecal	748:753	arg1	content					755:761	the caecal content	744:761	the caecal content	744:761	Bacterial population analysis reveals that these Enterobacteriaceae population in the caecal content decline by one order of magnitude after administration of POT complexes.
34740036	1	42	theme	dysbiotic	199:207	arg1	expansion					209:217	the dysbiotic expansion	195:217	the dysbiotic expansion of anaerobic bacteria of the Enterobacteriaceae family	195:272	Here we show that Preyssler-type polyoxotungstates (Preyssler-type POTs, [NaP5W30O110]-14) complexed with peptides can prevent the dysbiotic expansion of anaerobic bacteria of the Enterobacteriaceae family.
34740036	3	43	theme	73	580:581	arg1	%					582:582	%	582:582	%	582:582	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	2	44	theme	POT	416:418	arg1	complexes					420:428	POT complexes	416:428	POT complexes	416:428	In a dextran sulfate sodium (DSS)-induced colitis model, symptom remission of C57BL/6 J mice with colitis is achieved by orally treated with POT complexes.
34740036	3	45	theme	days	435:438	arg1	administration					449:462	Ten days of daily administration	431:462	Ten days of daily administration of POT complexes	431:479	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	0	46	theme	mice	58:61	arg1	gut					63:65	mice gut	58:65	mice gut	58:65	Amelioration of enteric dysbiosis by polyoxotungstates in mice gut.
34740036	4	47	theme	complexes	825:833	arg1	administration					803:816	administration	803:816	administration of POT complexes	803:833	Bacterial population analysis reveals that these Enterobacteriaceae population in the caecal content decline by one order of magnitude after administration of POT complexes.
34740036	3	48	theme	%	582:582	arg1	reduction					584:592	73% reduction	580:592	73% reduction for Tnf	580:600	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	3	48	theme	%	582:582	arg1	reduction					561:569	77% reduction	557:569	77% reduction for Il6	557:577	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	3	49	theme	markers	548:554	arg1	levels					522:527	the mRNA levels	513:527	the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon	513:659	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	3	49	theme	markers	548:554	arg1	loss					504:507	5% body weight loss	489:507	5% body weight loss	489:507	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	3	50	theme	POT	467:469	arg1	complexes					471:479	POT complexes	467:479	POT complexes	467:479	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	3	51	dep	caecum	635:640	arg1	the					631:633	the	631:633	the	631:633	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	4	52	theme	POT	821:823	arg1	complexes					825:833	POT complexes	821:833	POT complexes	821:833	Bacterial population analysis reveals that these Enterobacteriaceae population in the caecal content decline by one order of magnitude after administration of POT complexes.
34740036	2	53	with	remission	340:348	arg1	colitis					373:379	colitis	373:379	colitis	373:379	In a dextran sulfate sodium (DSS)-induced colitis model, symptom remission of C57BL/6 J mice with colitis is achieved by orally treated with POT complexes.
34740036	5	54	theme	gut	995:997	arg1	inflammation					999:1010	gut inflammation	995:1010	gut inflammation	995:1010	POT complexes exert anti-inflammatory effects indirectly on the host immune system by inhibition of malignant expansion of anaerobic Enterobacteriaceae during gut inflammation.
34740036	1	55	theme	Preyssler-type	120:133	arg1	[NaP5W30O110					141:152	Preyssler-type POTs, [NaP5W30O110]-14	120:156	[NaP5W30O110	141:152	Here we show that Preyssler-type polyoxotungstates (Preyssler-type POTs, [NaP5W30O110]-14) complexed with peptides can prevent the dysbiotic expansion of anaerobic bacteria of the Enterobacteriaceae family.
34740036	1	55	theme	Preyssler-type	120:133	arg1	polyoxotungstates					101:117	Preyssler-type polyoxotungstates	86:117	Preyssler-type polyoxotungstates (Preyssler-type POTs, [NaP5W30O110]-14) complexed with peptides	86:181	Here we show that Preyssler-type polyoxotungstates (Preyssler-type POTs, [NaP5W30O110]-14) complexed with peptides can prevent the dysbiotic expansion of anaerobic bacteria of the Enterobacteriaceae family.
34740036	7	56	theme	bacteria	1247:1254	arg1	inflammation					1281:1292	enteric bacteria dysbiosis-associated gut inflammation	1239:1292	enteric bacteria dysbiosis-associated gut inflammation	1239:1292	Rationally designed POT complexes will provide distinctive approach to improve enteric bacteria dysbiosis-associated gut inflammation by balancing bacterial communities.
34740036	3	57	theme	%	605:605	arg1	reduction					607:615	91% reduction	603:615	91% reduction for Cxcl1	603:625	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	3	57	theme	%	605:605	arg1	reduction					561:569	77% reduction	557:569	77% reduction for Il6	557:577	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	4	58	theme	Enterobacteriaceae	711:728	arg1	population					730:739	these Enterobacteriaceae population	705:739	these Enterobacteriaceae population in the caecal content	705:761	Bacterial population analysis reveals that these Enterobacteriaceae population in the caecal content decline by one order of magnitude after administration of POT complexes.
34740036	3	59	theme	%	559:559	arg1	reduction					584:592	73% reduction	580:592	73% reduction for Tnf	580:600	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	3	59	theme	%	559:559	arg1	reduction					607:615	91% reduction	603:615	91% reduction for Cxcl1	603:625	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	3	59	theme	%	559:559	arg1	reduction					561:569	77% reduction	557:569	77% reduction for Il6	557:577	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	1	60	theme	POTs	135:138	arg1	[NaP5W30O110					141:152	Preyssler-type POTs, [NaP5W30O110]-14	120:156	[NaP5W30O110	141:152	Here we show that Preyssler-type polyoxotungstates (Preyssler-type POTs, [NaP5W30O110]-14) complexed with peptides can prevent the dysbiotic expansion of anaerobic bacteria of the Enterobacteriaceae family.
34740036	1	60	theme	POTs	135:138	arg1	polyoxotungstates					101:117	Preyssler-type polyoxotungstates	86:117	Preyssler-type polyoxotungstates (Preyssler-type POTs, [NaP5W30O110]-14) complexed with peptides	86:181	Here we show that Preyssler-type polyoxotungstates (Preyssler-type POTs, [NaP5W30O110]-14) complexed with peptides can prevent the dysbiotic expansion of anaerobic bacteria of the Enterobacteriaceae family.
34740036	3	61	theme	mRNA	517:520	arg1	levels					522:527	the mRNA levels	513:527	the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon	513:659	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	5	62	theme	malignant	936:944	arg1	expansion					946:954	malignant expansion	936:954	malignant expansion of anaerobic Enterobacteriaceae during gut inflammation	936:1010	POT complexes exert anti-inflammatory effects indirectly on the host immune system by inhibition of malignant expansion of anaerobic Enterobacteriaceae during gut inflammation.
34740036	2	63	theme	-induced	308:315	arg1	model					325:329	a dextran sulfate sodium (DSS)-induced colitis model	278:329	a dextran sulfate sodium (DSS)-induced colitis model	278:329	In a dextran sulfate sodium (DSS)-induced colitis model, symptom remission of C57BL/6 J mice with colitis is achieved by orally treated with POT complexes.
34740036	1	64	theme	anaerobic	222:230	arg1	bacteria					232:239	anaerobic bacteria	222:239	anaerobic bacteria of the Enterobacteriaceae family	222:272	Here we show that Preyssler-type polyoxotungstates (Preyssler-type POTs, [NaP5W30O110]-14) complexed with peptides can prevent the dysbiotic expansion of anaerobic bacteria of the Enterobacteriaceae family.
34740036	7	65	theme	distinctive	1207:1217	arg1	approach					1219:1226	distinctive approach	1207:1226	distinctive approach to improve enteric bacteria dysbiosis-associated gut inflammation by balancing bacterial communities	1207:1327	Rationally designed POT complexes will provide distinctive approach to improve enteric bacteria dysbiosis-associated gut inflammation by balancing bacterial communities.
34740036	7	66	theme	bacterial	1307:1315	arg1	communities					1317:1327	bacterial communities	1307:1327	bacterial communities	1307:1327	Rationally designed POT complexes will provide distinctive approach to improve enteric bacteria dysbiosis-associated gut inflammation by balancing bacterial communities.
34740036	5	67	theme	expansion	946:954	arg1	inhibition					922:931	inhibition	922:931	inhibition of malignant expansion of anaerobic Enterobacteriaceae during gut inflammation	922:1010	POT complexes exert anti-inflammatory effects indirectly on the host immune system by inhibition of malignant expansion of anaerobic Enterobacteriaceae during gut inflammation.
34740036	4	68	from	population	730:739	arg1	content					755:761	the caecal content	744:761	the caecal content	744:761	Bacterial population analysis reveals that these Enterobacteriaceae population in the caecal content decline by one order of magnitude after administration of POT complexes.
34740036	2	69	theme	C57BL/6 J	353:361	arg1	mice					363:366	C57BL/6 J mice	353:366	C57BL/6 J mice	353:366	In a dextran sulfate sodium (DSS)-induced colitis model, symptom remission of C57BL/6 J mice with colitis is achieved by orally treated with POT complexes.
34740036	3	70	from	levels	522:527	arg1	caecum					635:640	caecum	635:640	caecum	635:640	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	3	70	from	levels	522:527	arg1	colon					655:659	proximal colon	646:659	proximal colon	646:659	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
34740036	1	71	theme	bacteria	232:239	arg1	expansion					209:217	the dysbiotic expansion	195:217	the dysbiotic expansion of anaerobic bacteria of the Enterobacteriaceae family	195:272	Here we show that Preyssler-type polyoxotungstates (Preyssler-type POTs, [NaP5W30O110]-14) complexed with peptides can prevent the dysbiotic expansion of anaerobic bacteria of the Enterobacteriaceae family.
34740036	6	72	theme	vitro	1077:1081	arg1	mice					1092:1095	vitro, healthy mice	1077:1095	mice	1092:1095	Furthermore, POTs show negligible effect on bacterial growth in vitro, healthy mice and their microbiota composition under homeostatic conditions.
34740036	3	73	theme	body	492:495	arg1	loss					504:507	5% body weight loss	489:507	5% body weight loss	489:507	Ten days of daily administration of POT complexes reduces 5% body weight loss and the mRNA levels of proinflammatory markers (77% reduction for Il6, 73% reduction for Tnf, 91% reduction for Cxcl1) in the caecum and proximal colon.
33794389	5	0	used	used	673:676	arg2	Gamma					655:659	Gamma	655:659	Gamma counter	655:667	Gamma counter was used to measure microbead encapsulation efficiency and Slit3 release.
33794389	5	1	theme	encapsulation	699:711	arg1	efficiency					713:722	microbead encapsulation efficiency	689:722	microbead encapsulation efficiency	689:722	Gamma counter was used to measure microbead encapsulation efficiency and Slit3 release.
33794389	4	2	theme	mannuronic	638:647	arg1	acid					649:652	low-viscosity high mannuronic acid	619:652	low-viscosity high mannuronic acid	619:652	METHODS Slit3 labeled with I-125 was encapsulated in microbeads composed of low-viscosity alginate of high-glucuronic acid content, first coated with poly-L-ornithine for various durations and finally with low-viscosity high mannuronic acid.
33794389	6	3	theme	human	858:862	arg1	vein					874:877	human umbilical vein and mouse endothelial cells	858:905	vein	874:877	Markers of angiogenesis were assessed with Boyden chamber, scratch wound, and Matrigel tube formation assays using human umbilical vein and mouse endothelial cells.
33794389	8	4	from	24	1481:1482	arg1	closure					1470:1476	wound closure	1464:1476	wound closure at 24 and 48 h	1464:1491	Compared with controls (media alone), Slit3 microbeads significantly inhibited in vitro cellular migration, endothelial cell migration for wound closure at 24 and 48 h and endothelial tube formation (P < 0.001, respectively).
33794389	7	5	theme	Slit3	1151:1155	arg1	encapsulation					1157:1169	Slit3 encapsulation	1151:1169	Slit3 encapsulation	1151:1169	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	2	6	theme	exact	290:294	arg1	mechanism					296:304	the exact mechanism	286:304	the exact mechanism	286:304	Slit3 proteins exhibit both proangiogenic and antiangiogenic properties, but the exact mechanism remains unclear.
33794389	7	7	theme	release	1142:1148	arg1	efficiency					1119:1128	encapsulation efficiency	1105:1128	encapsulation efficiency	1105:1128	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	7	7	theme	release	1142:1148	arg1	rate					1134:1137	rate	1134:1137	rate	1134:1137	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	6	8	theme	tube	830:833	arg1	assays					845:850	Matrigel tube formation assays	821:850	Matrigel tube formation assays	821:850	Markers of angiogenesis were assessed with Boyden chamber, scratch wound, and Matrigel tube formation assays using human umbilical vein and mouse endothelial cells.
33794389	8	9	from	48 h	1488:1491	arg1	closure					1470:1476	wound closure	1464:1476	wound closure at 24 and 48 h	1464:1491	Compared with controls (media alone), Slit3 microbeads significantly inhibited in vitro cellular migration, endothelial cell migration for wound closure at 24 and 48 h and endothelial tube formation (P < 0.001, respectively).
33794389	8	10	theme	tube	1509:1512	arg1	formation					1514:1522	endothelial tube formation	1497:1522	endothelial tube formation (P < 0.001, respectively)	1497:1548	Compared with controls (media alone), Slit3 microbeads significantly inhibited in vitro cellular migration, endothelial cell migration for wound closure at 24 and 48 h and endothelial tube formation (P < 0.001, respectively).
33794389	10	11	theme	tube	1728:1731	arg1	formation					1733:1741	in vitro endothelial tube formation	1707:1741	in vitro endothelial tube formation	1707:1741	Microbead encapsulation reduces in vitro endothelial tube formation and inhibits cellular migration to impair angiogenesis.
33794389	7	12	theme	low-viscosity	1215:1227	arg1	alginate					1229:1236	lower low-viscosity alginate	1209:1236	lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%)	1209:1290	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	10	13	theme	in vitro	1707:1714	arg1	formation					1733:1741	in vitro endothelial tube formation	1707:1741	in vitro endothelial tube formation	1707:1741	Microbead encapsulation reduces in vitro endothelial tube formation and inhibits cellular migration to impair angiogenesis.
33794389	7	14	dep	was	964:966	arg1	determined					1094:1103	determined	1094:1103	determined encapsulation efficiency and rate of release	1094:1148	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	9	15	theme	alginate	1654:1661	arg1	microbeads					1663:1672	alginate microbeads	1654:1672	alginate microbeads	1654:1672	CONCLUSIONS Slit3 can be effectively encapsulated and delivered via a controlled release pattern using alginate microbeads.
33794389	7	16	theme	Microbead	1072:1080	arg1	composition					1082:1092	6 to 12 d. Microbead composition	1061:1092	6 to 12 d. Microbead composition	1061:1092	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	1	17	theme	angiogenesis	150:161	arg1	regulator					137:145	a key regulator	131:145	a key regulator of angiogenesis and cellular function in experimental models	131:206	BACKGROUND The Slit-Robo pathway is a key regulator of angiogenesis and cellular function in experimental models.
33794389	1	17	theme	angiogenesis	150:161	arg1	pathway					120:126	The Slit-Robo pathway	106:126	The Slit-Robo pathway	106:126	BACKGROUND The Slit-Robo pathway is a key regulator of angiogenesis and cellular function in experimental models.
33794389	4	18	theme	acid	531:534	arg1	content					536:542	high-glucuronic acid content	515:542	high-glucuronic acid content	515:542	METHODS Slit3 labeled with I-125 was encapsulated in microbeads composed of low-viscosity alginate of high-glucuronic acid content, first coated with poly-L-ornithine for various durations and finally with low-viscosity high mannuronic acid.
33794389	1	19	dep	BACKGROUND	95:104	arg1	regulator					137:145	a key regulator	131:145	a key regulator of angiogenesis and cellular function in experimental models	131:206	BACKGROUND The Slit-Robo pathway is a key regulator of angiogenesis and cellular function in experimental models.
33794389	1	19	dep	BACKGROUND	95:104	arg1	pathway					120:126	The Slit-Robo pathway	106:126	The Slit-Robo pathway	106:126	BACKGROUND The Slit-Robo pathway is a key regulator of angiogenesis and cellular function in experimental models.
33794389	1	20	theme	cellular	167:174	arg1	function					176:183	cellular function	167:183	cellular function	167:183	BACKGROUND The Slit-Robo pathway is a key regulator of angiogenesis and cellular function in experimental models.
33794389	8	21	theme	cellular	1413:1420	arg1	migration					1422:1430	in vitro cellular migration	1404:1430	in vitro cellular migration	1404:1430	Compared with controls (media alone), Slit3 microbeads significantly inhibited in vitro cellular migration, endothelial cell migration for wound closure at 24 and 48 h and endothelial tube formation (P < 0.001, respectively).
33794389	7	22	theme	Slit3	1002:1006	arg1	release					991:997	an initial burst phase release	968:997	an initial burst phase release of Slit3	968:1006	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	8	23	dep	controls	1339:1346	arg1	media					1349:1353	media	1349:1353	media alone	1349:1359	Compared with controls (media alone), Slit3 microbeads significantly inhibited in vitro cellular migration, endothelial cell migration for wound closure at 24 and 48 h and endothelial tube formation (P < 0.001, respectively).
33794389	4	24	theme	low-viscosity	489:501	arg1	alginate					503:510	low-viscosity alginate	489:510	low-viscosity alginate of high-glucuronic acid content	489:542	METHODS Slit3 labeled with I-125 was encapsulated in microbeads composed of low-viscosity alginate of high-glucuronic acid content, first coated with poly-L-ornithine for various durations and finally with low-viscosity high mannuronic acid.
33794389	7	25	theme	content	1262:1268	arg1	concentrations					1270:1283	high-glucuronic acid content concentrations	1241:1283	high-glucuronic acid content concentrations (1.5%)	1241:1290	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	7	25	theme	content	1262:1268	arg1	%					1289:1289	1.5%	1286:1289	1.5%	1286:1289	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	9	26	theme	release	1632:1638	arg1	pattern					1640:1646	a controlled release pattern	1619:1646	a controlled release pattern using alginate microbeads	1619:1672	CONCLUSIONS Slit3 can be effectively encapsulated and delivered via a controlled release pattern using alginate microbeads.
33794389	8	27	theme	cell	1445:1448	arg1	migration					1450:1458	endothelial cell migration	1433:1458	endothelial cell migration for wound closure at 24 and 48 h	1433:1491	Compared with controls (media alone), Slit3 microbeads significantly inhibited in vitro cellular migration, endothelial cell migration for wound closure at 24 and 48 h and endothelial tube formation (P < 0.001, respectively).
33794389	0	28	theme	Controlled	0:9	arg1	Delivery					11:18	Controlled Delivery	0:18	Controlled Delivery of Slit3 Proteins from Alginate Microbeads	0:61	Controlled Delivery of Slit3 Proteins from Alginate Microbeads Inhibits In Vitro Angiogenesis.
33794389	7	29	theme	burst	979:983	arg1	release					991:997	an initial burst phase release	968:997	an initial burst phase release of Slit3	968:1006	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	3	30	theme	vascular	383:390	arg1	diseases					392:399	vascular diseases	383:399	vascular diseases	383:399	It is theorized that Slit3 may be a potential treatment for vascular diseases and cancer.
33794389	4	31	theme	various	584:590	arg1	durations					592:600	various durations	584:600	various durations	584:600	METHODS Slit3 labeled with I-125 was encapsulated in microbeads composed of low-viscosity alginate of high-glucuronic acid content, first coated with poly-L-ornithine for various durations and finally with low-viscosity high mannuronic acid.
33794389	7	32	theme	high-glucuronic	1241:1255	arg1	concentrations					1270:1283	high-glucuronic acid content concentrations	1241:1283	high-glucuronic acid content concentrations (1.5%)	1241:1290	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	7	32	theme	high-glucuronic	1241:1255	arg1	%					1289:1289	1.5%	1286:1289	1.5%	1286:1289	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	0	33	theme	Proteins	29:36	arg1	Delivery					11:18	Controlled Delivery	0:18	Controlled Delivery of Slit3 Proteins from Alginate Microbeads	0:61	Controlled Delivery of Slit3 Proteins from Alginate Microbeads Inhibits In Vitro Angiogenesis.
33794389	7	34	theme	lower	1209:1213	arg1	alginate					1229:1236	lower low-viscosity alginate	1209:1236	lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%)	1209:1290	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	7	35	dep	RESULTS	908:914	arg1	was					964:966	was	964:966	was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release	964:1148	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	0	36	theme	Alginate	43:50	arg1	Microbeads					52:61	Alginate Microbeads	43:61	Alginate Microbeads	43:61	Controlled Delivery of Slit3 Proteins from Alginate Microbeads Inhibits In Vitro Angiogenesis.
33794389	8	37	dep	formation	1514:1522	arg1	P < 0.001					1525:1533	P < 0.001	1525:1533	P < 0.001	1525:1533	Compared with controls (media alone), Slit3 microbeads significantly inhibited in vitro cellular migration, endothelial cell migration for wound closure at 24 and 48 h and endothelial tube formation (P < 0.001, respectively).
33794389	5	38	dep	Gamma	655:659	arg1	counter					661:667	counter	661:667	counter	661:667	Gamma counter was used to measure microbead encapsulation efficiency and Slit3 release.
33794389	7	39	from	efficient	1180:1188	arg1	microbeads					1193:1202	microbeads	1193:1202	microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating	1193:1322	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	6	40	theme	scratch	802:808	arg1	wound					810:814	scratch wound	802:814	scratch wound	802:814	Markers of angiogenesis were assessed with Boyden chamber, scratch wound, and Matrigel tube formation assays using human umbilical vein and mouse endothelial cells.
33794389	7	41	theme	initial	971:977	arg1	release					991:997	an initial burst phase release	968:997	an initial burst phase release of Slit3	968:1006	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	4	42	dep	encapsulated	450:461	arg1	coated					551:556	coated	551:556	coated with poly-L-ornithine for various durations and finally with low-viscosity high mannuronic acid	551:652	METHODS Slit3 labeled with I-125 was encapsulated in microbeads composed of low-viscosity alginate of high-glucuronic acid content, first coated with poly-L-ornithine for various durations and finally with low-viscosity high mannuronic acid.
33794389	6	43	theme	mouse	883:887	arg1	cells					901:905	human umbilical vein and mouse endothelial cells	858:905	cells	901:905	Markers of angiogenesis were assessed with Boyden chamber, scratch wound, and Matrigel tube formation assays using human umbilical vein and mouse endothelial cells.
33794389	8	44	theme	endothelial	1497:1507	arg1	formation					1514:1522	endothelial tube formation	1497:1522	endothelial tube formation (P < 0.001, respectively)	1497:1548	Compared with controls (media alone), Slit3 microbeads significantly inhibited in vitro cellular migration, endothelial cell migration for wound closure at 24 and 48 h and endothelial tube formation (P < 0.001, respectively).
33794389	5	45	theme	microbead	689:697	arg1	efficiency					713:722	microbead encapsulation efficiency	689:722	microbead encapsulation efficiency	689:722	Gamma counter was used to measure microbead encapsulation efficiency and Slit3 release.
33794389	7	46	from	microbeads	1193:1202	arg1	efficient					1180:1188	efficient	1180:1188	efficient	1180:1188	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	4	47	theme	METHODS	413:419	arg1	Slit3					421:425	METHODS Slit3	413:425	METHODS Slit3 labeled with I-125	413:444	METHODS Slit3 labeled with I-125 was encapsulated in microbeads composed of low-viscosity alginate of high-glucuronic acid content, first coated with poly-L-ornithine for various durations and finally with low-viscosity high mannuronic acid.
33794389	7	48	theme	poly-L-ornithine	1299:1314	arg1	coating					1316:1322	no poly-L-ornithine coating	1296:1322	no poly-L-ornithine coating	1296:1322	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	7	49	dep	12 d.	1066:1070	arg1	to					1063:1064	to	1063:1064	to	1063:1064	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	6	50	theme	umbilical	864:872	arg1	vein					874:877	human umbilical vein and mouse endothelial cells	858:905	vein	874:877	Markers of angiogenesis were assessed with Boyden chamber, scratch wound, and Matrigel tube formation assays using human umbilical vein and mouse endothelial cells.
33794389	7	51	theme	Slit3-loaded	933:944	arg1	microbeads					946:955	Slit3-loaded microbeads	933:955	Slit3-loaded microbeads	933:955	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	7	52	with	microbeads	1193:1202	arg1	coating					1316:1322	no poly-L-ornithine coating	1296:1322	no poly-L-ornithine coating	1296:1322	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	7	52	with	microbeads	1193:1202	arg1	alginate					1229:1236	lower low-viscosity alginate	1209:1236	lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%)	1209:1290	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	1	53	theme	function	176:183	arg1	regulator					137:145	a key regulator	131:145	a key regulator of angiogenesis and cellular function in experimental models	131:206	BACKGROUND The Slit-Robo pathway is a key regulator of angiogenesis and cellular function in experimental models.
33794389	1	53	theme	function	176:183	arg1	pathway					120:126	The Slit-Robo pathway	106:126	The Slit-Robo pathway	106:126	BACKGROUND The Slit-Robo pathway is a key regulator of angiogenesis and cellular function in experimental models.
33794389	6	54	theme	formation	835:843	arg1	assays					845:850	Matrigel tube formation assays	821:850	Matrigel tube formation assays	821:850	Markers of angiogenesis were assessed with Boyden chamber, scratch wound, and Matrigel tube formation assays using human umbilical vein and mouse endothelial cells.
33794389	1	55	theme	experimental	188:199	arg1	models					201:206	experimental models	188:206	experimental models	188:206	BACKGROUND The Slit-Robo pathway is a key regulator of angiogenesis and cellular function in experimental models.
33794389	10	56	theme	Microbead	1675:1683	arg1	encapsulation					1685:1697	Microbead encapsulation	1675:1697	Microbead encapsulation	1675:1697	Microbead encapsulation reduces in vitro endothelial tube formation and inhibits cellular migration to impair angiogenesis.
33794389	6	57	theme	Matrigel	821:828	arg1	assays					845:850	Matrigel tube formation assays	821:850	Matrigel tube formation assays	821:850	Markers of angiogenesis were assessed with Boyden chamber, scratch wound, and Matrigel tube formation assays using human umbilical vein and mouse endothelial cells.
33794389	7	58	theme	encapsulation	1105:1117	arg1	efficiency					1119:1128	encapsulation efficiency	1105:1128	encapsulation efficiency	1105:1128	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	2	59	theme	antiangiogenic	255:268	arg1	properties					270:279	both proangiogenic and antiangiogenic properties	232:279	both proangiogenic and antiangiogenic properties	232:279	Slit3 proteins exhibit both proangiogenic and antiangiogenic properties, but the exact mechanism remains unclear.
33794389	10	60	theme	endothelial	1716:1726	arg1	formation					1733:1741	in vitro endothelial tube formation	1707:1741	in vitro endothelial tube formation	1707:1741	Microbead encapsulation reduces in vitro endothelial tube formation and inhibits cellular migration to impair angiogenesis.
33794389	5	61	theme	Slit3	728:732	arg1	release					734:740	Slit3 release	728:740	Slit3 release	728:740	Gamma counter was used to measure microbead encapsulation efficiency and Slit3 release.
33794389	2	62	theme	proangiogenic	237:249	arg1	properties					270:279	both proangiogenic and antiangiogenic properties	232:279	both proangiogenic and antiangiogenic properties	232:279	Slit3 proteins exhibit both proangiogenic and antiangiogenic properties, but the exact mechanism remains unclear.
33794389	9	63	theme	CONCLUSIONS	1551:1561	arg1	Slit3					1563:1567	CONCLUSIONS Slit3	1551:1567	CONCLUSIONS Slit3	1551:1567	CONCLUSIONS Slit3 can be effectively encapsulated and delivered via a controlled release pattern using alginate microbeads.
33794389	1	64	theme	key	133:135	arg1	regulator					137:145	a key regulator	131:145	a key regulator of angiogenesis and cellular function in experimental models	131:206	BACKGROUND The Slit-Robo pathway is a key regulator of angiogenesis and cellular function in experimental models.
33794389	1	64	theme	key	133:135	arg1	pathway					120:126	The Slit-Robo pathway	106:126	The Slit-Robo pathway	106:126	BACKGROUND The Slit-Robo pathway is a key regulator of angiogenesis and cellular function in experimental models.
33794389	0	65	theme	In Vitro	72:79	arg1	Angiogenesis					81:92	In Vitro Angiogenesis	72:92	In Vitro Angiogenesis	72:92	Controlled Delivery of Slit3 Proteins from Alginate Microbeads Inhibits In Vitro Angiogenesis.
33794389	7	66	theme	sustained	1039:1047	arg1	release					1049:1055	sustained release	1039:1055	sustained release for 6 to 12 d. Microbead composition	1039:1092	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	4	67	theme	content	536:542	arg1	alginate					503:510	low-viscosity alginate	489:510	low-viscosity alginate of high-glucuronic acid content	489:542	METHODS Slit3 labeled with I-125 was encapsulated in microbeads composed of low-viscosity alginate of high-glucuronic acid content, first coated with poly-L-ornithine for various durations and finally with low-viscosity high mannuronic acid.
33794389	8	68	theme	Slit3	1363:1367	arg1	microbeads					1369:1378	Slit3 microbeads	1363:1378	Slit3 microbeads	1363:1378	Compared with controls (media alone), Slit3 microbeads significantly inhibited in vitro cellular migration, endothelial cell migration for wound closure at 24 and 48 h and endothelial tube formation (P < 0.001, respectively).
33794389	11	69	theme	therapeutic	1847:1857	arg1	option					1859:1864	a therapeutic option	1845:1864	a therapeutic option to mitigate tumor proliferation	1845:1896	Thus, Slit3 microparticles may be explored as a therapeutic option to mitigate tumor proliferation.
33794389	11	69	theme	therapeutic	1847:1857	arg1	microparticles					1811:1824	Slit3 microparticles	1805:1824	Slit3 microparticles	1805:1824	Thus, Slit3 microparticles may be explored as a therapeutic option to mitigate tumor proliferation.
33794389	7	70	theme	concentrations	1270:1283	arg1	coating					1316:1322	no poly-L-ornithine coating	1296:1322	no poly-L-ornithine coating	1296:1322	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	7	70	theme	concentrations	1270:1283	arg1	alginate					1229:1236	lower low-viscosity alginate	1209:1236	lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%)	1209:1290	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	7	71	theme	first	1016:1020	arg1	24 h					1022:1025	the first 24 h	1012:1025	the first 24 h followed by sustained release for 6 to 12 d. Microbead composition	1012:1092	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	0	72	from	Microbeads	52:61	arg1	Delivery					11:18	Controlled Delivery	0:18	Controlled Delivery of Slit3 Proteins from Alginate Microbeads	0:61	Controlled Delivery of Slit3 Proteins from Alginate Microbeads Inhibits In Vitro Angiogenesis.
33794389	8	73	theme	in vitro	1404:1411	arg1	migration					1422:1430	in vitro cellular migration	1404:1430	in vitro cellular migration	1404:1430	Compared with controls (media alone), Slit3 microbeads significantly inhibited in vitro cellular migration, endothelial cell migration for wound closure at 24 and 48 h and endothelial tube formation (P < 0.001, respectively).
33794389	11	74	theme	tumor	1878:1882	arg1	proliferation					1884:1896	tumor proliferation	1878:1896	tumor proliferation	1878:1896	Thus, Slit3 microparticles may be explored as a therapeutic option to mitigate tumor proliferation.
33794389	7	75	theme	acid	1257:1260	arg1	concentrations					1270:1283	high-glucuronic acid content concentrations	1241:1283	high-glucuronic acid content concentrations (1.5%)	1241:1290	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	7	75	theme	acid	1257:1260	arg1	%					1289:1289	1.5%	1286:1289	1.5%	1286:1289	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	9	76	theme	controlled	1621:1630	arg1	pattern					1640:1646	a controlled release pattern	1619:1646	a controlled release pattern using alginate microbeads	1619:1672	CONCLUSIONS Slit3 can be effectively encapsulated and delivered via a controlled release pattern using alginate microbeads.
33794389	3	77	theme	potential	359:367	arg1	treatment					369:377	a potential treatment	357:377	a potential treatment for vascular diseases and cancer	357:410	It is theorized that Slit3 may be a potential treatment for vascular diseases and cancer.
33794389	3	77	theme	potential	359:367	arg1	Slit3					344:348	Slit3	344:348	Slit3	344:348	It is theorized that Slit3 may be a potential treatment for vascular diseases and cancer.
33794389	4	78	theme	high-glucuronic	515:529	arg1	content					536:542	high-glucuronic acid content	515:542	high-glucuronic acid content	515:542	METHODS Slit3 labeled with I-125 was encapsulated in microbeads composed of low-viscosity alginate of high-glucuronic acid content, first coated with poly-L-ornithine for various durations and finally with low-viscosity high mannuronic acid.
33794389	6	79	theme	angiogenesis	754:765	arg1	Markers					743:749	Markers	743:749	Markers of angiogenesis	743:765	Markers of angiogenesis were assessed with Boyden chamber, scratch wound, and Matrigel tube formation assays using human umbilical vein and mouse endothelial cells.
33794389	10	80	theme	cellular	1756:1763	arg1	migration					1765:1773	cellular migration	1756:1773	cellular migration to impair angiogenesis	1756:1796	Microbead encapsulation reduces in vitro endothelial tube formation and inhibits cellular migration to impair angiogenesis.
33794389	1	81	from	regulator	137:145	arg1	models					201:206	experimental models	188:206	experimental models	188:206	BACKGROUND The Slit-Robo pathway is a key regulator of angiogenesis and cellular function in experimental models.
33794389	8	82	theme	endothelial	1433:1443	arg1	migration					1450:1458	endothelial cell migration	1433:1458	endothelial cell migration for wound closure at 24 and 48 h	1433:1491	Compared with controls (media alone), Slit3 microbeads significantly inhibited in vitro cellular migration, endothelial cell migration for wound closure at 24 and 48 h and endothelial tube formation (P < 0.001, respectively).
33794389	0	83	theme	Slit3	23:27	arg1	Proteins					29:36	Slit3 Proteins	23:36	Slit3 Proteins	23:36	Controlled Delivery of Slit3 Proteins from Alginate Microbeads Inhibits In Vitro Angiogenesis.
33794389	7	84	theme	phase	985:989	arg1	release					991:997	an initial burst phase release	968:997	an initial burst phase release of Slit3	968:1006	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
33794389	1	85	theme	Slit-Robo	110:118	arg1	regulator					137:145	a key regulator	131:145	a key regulator of angiogenesis and cellular function in experimental models	131:206	BACKGROUND The Slit-Robo pathway is a key regulator of angiogenesis and cellular function in experimental models.
33794389	1	85	theme	Slit-Robo	110:118	arg1	pathway					120:126	The Slit-Robo pathway	106:126	The Slit-Robo pathway	106:126	BACKGROUND The Slit-Robo pathway is a key regulator of angiogenesis and cellular function in experimental models.
33794389	11	86	theme	Slit3	1805:1809	arg1	option					1859:1864	a therapeutic option	1845:1864	a therapeutic option to mitigate tumor proliferation	1845:1896	Thus, Slit3 microparticles may be explored as a therapeutic option to mitigate tumor proliferation.
33794389	11	86	theme	Slit3	1805:1809	arg1	microparticles					1811:1824	Slit3 microparticles	1805:1824	Slit3 microparticles	1805:1824	Thus, Slit3 microparticles may be explored as a therapeutic option to mitigate tumor proliferation.
33794389	8	87	theme	wound	1464:1468	arg1	closure					1470:1476	wound closure	1464:1476	wound closure at 24 and 48 h	1464:1491	Compared with controls (media alone), Slit3 microbeads significantly inhibited in vitro cellular migration, endothelial cell migration for wound closure at 24 and 48 h and endothelial tube formation (P < 0.001, respectively).
33794389	6	88	theme	endothelial	889:899	arg1	cells					901:905	human umbilical vein and mouse endothelial cells	858:905	cells	901:905	Markers of angiogenesis were assessed with Boyden chamber, scratch wound, and Matrigel tube formation assays using human umbilical vein and mouse endothelial cells.
33794389	2	89	theme	Slit3	209:213	arg1	proteins					215:222	Slit3 proteins	209:222	Slit3 proteins	209:222	Slit3 proteins exhibit both proangiogenic and antiangiogenic properties, but the exact mechanism remains unclear.
33794389	6	90	theme	Boyden	786:791	arg1	chamber					793:799	Boyden chamber	786:799	Boyden chamber	786:799	Markers of angiogenesis were assessed with Boyden chamber, scratch wound, and Matrigel tube formation assays using human umbilical vein and mouse endothelial cells.
33794389	7	91	theme	microbeads	946:955	arg1	incubation					919:928	incubation	919:928	incubation of Slit3-loaded microbeads	919:955	RESULTS On incubation of Slit3-loaded microbeads, there was an initial burst phase release of Slit3 for the first 24 h followed by sustained release for 6 to 12 d. Microbead composition determined encapsulation efficiency and rate of release; Slit3 encapsulation was most efficient in microbeads with lower low-viscosity alginate of high-glucuronic acid content concentrations (1.5%) and no poly-L-ornithine coating.
32231564	11	0	theme	BBR	2032:2034	arg1	treatment					2036:2044	BBR treatment	2032:2044	BBR treatment	2032:2044	Metagenomic analysis further showed an elevated potential for lipid and glycan metabolism and synthesis of SCFAs, as well as reduced potential of TMAO production after BBR treatment.
32231564	6	1	located	observed	1134:1141	arg2	factor-alpha					1028:1039	tumor necrosis factor-alpha	1013:1039	tumor necrosis factor-alpha	1013:1039	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	6	1	located	observed	1134:1141	arg1	group					1164:1168	the high-dose BBR group	1146:1168	the high-dose BBR group	1146:1168	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	6	1	located	observed	1134:1141	arg2	IL-10					1100:1104	increased anti-inflammatory IL-10	1072:1104	increased anti-inflammatory IL-10	1072:1104	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	6	1	located	observed	1134:1141	arg2	IL-6					1063:1066	IL-6	1063:1066	IL-6	1063:1066	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	6	1	located	observed	1134:1141	arg2	IL					1055:1056	interleukin (IL)-1β	1042:1060	interleukin (IL)-1β	1042:1060	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	6	1	located	observed	1134:1141	arg2	cytokines					1003:1011	Decreased pro-inflammatory cytokines	976:1011	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels	976:1127	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	10	2	theme	BBR	1853:1855	arg1	doses					1857:1861	both BBR doses	1848:1861	both BBR doses	1848:1861	Alistipes and Roseburia were significantly enriched in high-dose BBR group while Blautia and Allobaculum were more enriched in low-dose, and Turicibacter was enriched in both BBR doses.
32231564	9	3	theme	short-chain	1603:1613	arg1	SCFAs					1628:1632	SCFAs	1628:1632	SCFAs	1628:1632	These microbiota displayed good anti-inflammatory effects related to the production of short-chain fatty acids (SCFAs) and were related to glucolipid metabolism.
32231564	9	3	theme	short-chain	1603:1613	arg1	acids					1621:1625	short-chain fatty acids	1603:1625	short-chain fatty acids (SCFAs)	1603:1633	These microbiota displayed good anti-inflammatory effects related to the production of short-chain fatty acids (SCFAs) and were related to glucolipid metabolism.
32231564	2	4	from	role	307:310	arg1	gut					354:356	gut	354:356	gut	354:356	Recently, the role of berberine (BBR) in atherosclerosis and gut microbiota has begun to be appreciated.
32231564	2	4	from	role	307:310	arg1	atherosclerosis					334:348	atherosclerosis	334:348	atherosclerosis	334:348	Recently, the role of berberine (BBR) in atherosclerosis and gut microbiota has begun to be appreciated.
32231564	11	5	theme	SCFAs	1971:1975	arg1	synthesis					1958:1966	synthesis	1958:1966	synthesis of SCFAs	1958:1975	Metagenomic analysis further showed an elevated potential for lipid and glycan metabolism and synthesis of SCFAs, as well as reduced potential of TMAO production after BBR treatment.
32231564	11	5	theme	SCFAs	1971:1975	arg1	metabolism					1943:1952	lipid and glycan metabolism	1926:1952	metabolism	1943:1952	Metagenomic analysis further showed an elevated potential for lipid and glycan metabolism and synthesis of SCFAs, as well as reduced potential of TMAO production after BBR treatment.
32231564	9	6	theme	acids	1621:1625	arg1	production					1589:1598	the production	1585:1598	the production of short-chain fatty acids (SCFAs)	1585:1633	These microbiota displayed good anti-inflammatory effects related to the production of short-chain fatty acids (SCFAs) and were related to glucolipid metabolism.
32231564	7	7	theme	microbiota	1358:1367	arg1	structure					1341:1349	the community compositional structure	1313:1349	the community compositional structure of gut microbiota	1313:1367	16S rRNA sequencing showed that BBR significantly altered the community compositional structure of gut microbiota.
32231564	3	8	theme	BBR	477:479	arg1	doses					468:472	high or low doses	456:472	high or low doses of BBR	456:479	The purposes of this study were to observe the effects of high or low doses of BBR on atherosclerosis and gut microbiota modulation, and to explore their correlation in ApoE-/- mice fed a high-fat diet.
32231564	13	9	theme	high	2274:2277	arg1	dose					2279:2282	the high dose	2270:2282	the high dose	2270:2282	The effects are more pronounced for the high dose.
32231564	14	10	theme	gut	2393:2395	arg1	microbiota					2397:2406	gut microbiota	2393:2406	gut microbiota	2393:2406	This anti-atherosclerotic effect of BBR may be partly attributed to changes in composition and functions of gut microbiota which may be associated with anti-inflammatory and metabolism of glucose and lipid.
32231564	6	11	theme	adiponectin	1110:1120	arg1	levels					1122:1127	adiponectin levels	1110:1127	adiponectin levels	1110:1127	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	4	12	theme	significant	603:613	arg1	decrease					615:622	A significant decrease	601:622	A significant decrease in atherosclerotic lesions	601:649	A significant decrease in atherosclerotic lesions was observed after treatment with BBR, with the effect of the high dose being more obvious.
32231564	3	13	from	effects	445:451	arg1	atherosclerosis					484:498	atherosclerosis	484:498	atherosclerosis	484:498	The purposes of this study were to observe the effects of high or low doses of BBR on atherosclerosis and gut microbiota modulation, and to explore their correlation in ApoE-/- mice fed a high-fat diet.
32231564	3	13	from	effects	445:451	arg1	modulation					519:528	gut microbiota modulation	504:528	gut microbiota modulation	504:528	The purposes of this study were to observe the effects of high or low doses of BBR on atherosclerosis and gut microbiota modulation, and to explore their correlation in ApoE-/- mice fed a high-fat diet.
32231564	10	14	theme	high-dose	1733:1741	arg1	group					1747:1751	high-dose BBR group	1733:1751	high-dose BBR group	1733:1751	Alistipes and Roseburia were significantly enriched in high-dose BBR group while Blautia and Allobaculum were more enriched in low-dose, and Turicibacter was enriched in both BBR doses.
32231564	7	15	theme	compositional	1327:1339	arg1	structure					1341:1349	the community compositional structure	1313:1349	the community compositional structure of gut microbiota	1313:1367	16S rRNA sequencing showed that BBR significantly altered the community compositional structure of gut microbiota.
32231564	3	16	theme	gut	504:506	arg1	modulation					519:528	gut microbiota modulation	504:528	gut microbiota modulation	504:528	The purposes of this study were to observe the effects of high or low doses of BBR on atherosclerosis and gut microbiota modulation, and to explore their correlation in ApoE-/- mice fed a high-fat diet.
32231564	15	17	theme	BBR	2560:2562	arg1	dose					2564:2567	BBR dose	2560:2567	BBR dose	2560:2567	Notably, gut microbiota alterations showed different sensitivity to BBR dose.
32231564	11	18	theme	reduced	1989:1995	arg1	potential					1997:2005	reduced potential	1989:2005	reduced potential of TMAO production	1989:2024	Metagenomic analysis further showed an elevated potential for lipid and glycan metabolism and synthesis of SCFAs, as well as reduced potential of TMAO production after BBR treatment.
32231564	6	19	theme	BBR	1250:1252	arg1	low-dose					1238:1245	low-dose	1238:1245	low-dose	1238:1245	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	5	20	theme	total	785:789	arg1	cholesterol					791:801	total cholesterol	785:801	total cholesterol	785:801	Both BBR treatments significantly reduced total cholesterol, APOB100, and very low-density lipoprotein cholesterol levels but levels of high/low-density lipoprotein cholesterol and lipoprotein (a) were only reduced by high-dose BBR.
32231564	9	21	theme	glucolipid	1655:1664	arg1	metabolism					1666:1675	glucolipid metabolism	1655:1675	glucolipid metabolism	1655:1675	These microbiota displayed good anti-inflammatory effects related to the production of short-chain fatty acids (SCFAs) and were related to glucolipid metabolism.
32231564	0	22	from	Modulation	58:67	arg1	Mice					120:123	High-Fat Diet-Fed ApoE-/- Mice	94:123	High-Fat Diet-Fed ApoE-/- Mice	94:123	Effect of Berberine on Atherosclerosis and Gut Microbiota Modulation and Their Correlation in High-Fat Diet-Fed ApoE-/- Mice.
32231564	11	23	theme	elevated	1903:1910	arg1	potential					1912:1920	an elevated potential	1900:1920	an elevated potential for lipid and glycan metabolism and synthesis of SCFAs	1900:1975	Metagenomic analysis further showed an elevated potential for lipid and glycan metabolism and synthesis of SCFAs, as well as reduced potential of TMAO production after BBR treatment.
32231564	11	24	theme	production	2015:2024	arg1	potential					1912:1920	an elevated potential	1900:1920	an elevated potential for lipid and glycan metabolism and synthesis of SCFAs	1900:1975	Metagenomic analysis further showed an elevated potential for lipid and glycan metabolism and synthesis of SCFAs, as well as reduced potential of TMAO production after BBR treatment.
32231564	11	24	theme	production	2015:2024	arg1	potential					1997:2005	reduced potential	1989:2005	reduced potential of TMAO production	1989:2024	Metagenomic analysis further showed an elevated potential for lipid and glycan metabolism and synthesis of SCFAs, as well as reduced potential of TMAO production after BBR treatment.
32231564	3	25	theme	ApoE-/-	567:573	arg1	mice					575:578	ApoE-/- mice	567:578	ApoE-/- mice fed a high-fat diet	567:598	The purposes of this study were to observe the effects of high or low doses of BBR on atherosclerosis and gut microbiota modulation, and to explore their correlation in ApoE-/- mice fed a high-fat diet.
32231564	15	26	theme	gut	2501:2503	arg1	alterations					2516:2526	gut microbiota alterations	2501:2526	gut microbiota alterations	2501:2526	Notably, gut microbiota alterations showed different sensitivity to BBR dose.
32231564	6	27	theme	high-dose	1150:1158	arg1	group					1164:1168	the high-dose BBR group	1146:1168	the high-dose BBR group	1146:1168	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	5	28	theme	BBR	748:750	arg1	treatments					752:761	Both BBR treatments	743:761	Both BBR treatments	743:761	Both BBR treatments significantly reduced total cholesterol, APOB100, and very low-density lipoprotein cholesterol levels but levels of high/low-density lipoprotein cholesterol and lipoprotein (a) were only reduced by high-dose BBR.
32231564	1	29	theme	associated	150:159	arg1	Atherosclerosis					126:140	Atherosclerosis	126:140	Atherosclerosis	126:140	Atherosclerosis and its associated cardiovascular diseases (CVDs) are serious threats to human health and have been reported to be associated with the gut microbiota.
32231564	1	29	theme	associated	150:159	arg1	threats					204:210	serious threats	196:210	serious threats to human health	196:226	Atherosclerosis and its associated cardiovascular diseases (CVDs) are serious threats to human health and have been reported to be associated with the gut microbiota.
32231564	1	29	theme	associated	150:159	arg1	CVDs					186:189	CVDs	186:189	CVDs	186:189	Atherosclerosis and its associated cardiovascular diseases (CVDs) are serious threats to human health and have been reported to be associated with the gut microbiota.
32231564	1	29	theme	associated	150:159	arg1	diseases					176:183	its associated cardiovascular diseases	146:183	its associated cardiovascular diseases (CVDs)	146:190	Atherosclerosis and its associated cardiovascular diseases (CVDs) are serious threats to human health and have been reported to be associated with the gut microbiota.
32231564	5	30	theme	lipoprotein	834:844	arg1	cholesterol					846:856	low-density lipoprotein cholesterol	822:856	low-density lipoprotein cholesterol	822:856	Both BBR treatments significantly reduced total cholesterol, APOB100, and very low-density lipoprotein cholesterol levels but levels of high/low-density lipoprotein cholesterol and lipoprotein (a) were only reduced by high-dose BBR.
32231564	0	31	from	Effect	0:5	arg1	Modulation					58:67	Atherosclerosis and Gut Microbiota Modulation	23:67	Atherosclerosis and Gut Microbiota Modulation	23:67	Effect of Berberine on Atherosclerosis and Gut Microbiota Modulation and Their Correlation in High-Fat Diet-Fed ApoE-/- Mice.
32231564	0	31	from	Effect	0:5	arg1	Correlation					79:89	Their Correlation	73:89	Their Correlation in High-Fat Diet-Fed ApoE-/- Mice	73:123	Effect of Berberine on Atherosclerosis and Gut Microbiota Modulation and Their Correlation in High-Fat Diet-Fed ApoE-/- Mice.
32231564	5	32	theme	lipoprotein	924:934	arg1	levels					869:874	levels	869:874	levels of high/low-density lipoprotein cholesterol and lipoprotein (a)	869:938	Both BBR treatments significantly reduced total cholesterol, APOB100, and very low-density lipoprotein cholesterol levels but levels of high/low-density lipoprotein cholesterol and lipoprotein (a) were only reduced by high-dose BBR.
32231564	12	33	theme	high-fat	2203:2210	arg1	diet					2212:2215	high-fat diet	2203:2215	high-fat diet in ApoE-/- mice	2203:2231	The findings demonstrate that both high and low-dose BBR can improve serum lipid and systemic inflammation levels, and alleviate atherosclerosis induced by high-fat diet in ApoE-/- mice.
32231564	2	34	dep	atherosclerosis	334:348	arg1	microbiota					358:367	microbiota	358:367	microbiota	358:367	Recently, the role of berberine (BBR) in atherosclerosis and gut microbiota has begun to be appreciated.
32231564	3	35	from	correlation	552:562	arg1	mice					575:578	ApoE-/- mice	567:578	ApoE-/- mice fed a high-fat diet	567:598	The purposes of this study were to observe the effects of high or low doses of BBR on atherosclerosis and gut microbiota modulation, and to explore their correlation in ApoE-/- mice fed a high-fat diet.
32231564	0	36	theme	Diet-Fed	103:110	arg1	Mice					120:123	High-Fat Diet-Fed ApoE-/- Mice	94:123	High-Fat Diet-Fed ApoE-/- Mice	94:123	Effect of Berberine on Atherosclerosis and Gut Microbiota Modulation and Their Correlation in High-Fat Diet-Fed ApoE-/- Mice.
32231564	7	37	theme	rRNA	1259:1262	arg1	sequencing					1264:1273	16S rRNA sequencing	1255:1273	16S rRNA sequencing	1255:1273	16S rRNA sequencing showed that BBR significantly altered the community compositional structure of gut microbiota.
32231564	8	38	theme	Turicibacter	1462:1473	arg1	abundance					1401:1409	the abundance	1397:1409	the abundance of Roseburia, Blautia, Allobaculum, Alistipes, and Turicibacter	1397:1473	Specifically, BBR enriched the abundance of Roseburia, Blautia, Allobaculum, Alistipes, and Turicibacter, and changed the abundance of Bilophila.
32231564	5	39	theme	high/low-density	879:894	arg1	cholesterol					908:918	high/low-density lipoprotein cholesterol	879:918	high/low-density lipoprotein cholesterol	879:918	Both BBR treatments significantly reduced total cholesterol, APOB100, and very low-density lipoprotein cholesterol levels but levels of high/low-density lipoprotein cholesterol and lipoprotein (a) were only reduced by high-dose BBR.
32231564	6	40	theme	necrosis	1019:1026	arg1	cytokines					1003:1011	Decreased pro-inflammatory cytokines	976:1011	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels	976:1127	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	6	40	theme	necrosis	1019:1026	arg1	factor-alpha					1028:1039	tumor necrosis factor-alpha	1013:1039	tumor necrosis factor-alpha	1013:1039	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	0	41	theme	Berberine	10:18	arg1	Effect					0:5	Effect	0:5	Effect of Berberine on Atherosclerosis and Gut Microbiota Modulation and Their Correlation in High-Fat Diet-Fed ApoE-/- Mice.	0:124	Effect of Berberine on Atherosclerosis and Gut Microbiota Modulation and Their Correlation in High-Fat Diet-Fed ApoE-/- Mice.
32231564	1	42	theme	gut	277:279	arg1	microbiota					281:290	the gut microbiota	273:290	the gut microbiota	273:290	Atherosclerosis and its associated cardiovascular diseases (CVDs) are serious threats to human health and have been reported to be associated with the gut microbiota.
32231564	5	43	theme	cholesterol	908:918	arg1	levels					869:874	levels	869:874	levels of high/low-density lipoprotein cholesterol and lipoprotein (a)	869:938	Both BBR treatments significantly reduced total cholesterol, APOB100, and very low-density lipoprotein cholesterol levels but levels of high/low-density lipoprotein cholesterol and lipoprotein (a) were only reduced by high-dose BBR.
32231564	10	44	from	enriched	1793:1800	arg1	low-dose					1805:1812	low-dose	1805:1812	low-dose	1805:1812	Alistipes and Roseburia were significantly enriched in high-dose BBR group while Blautia and Allobaculum were more enriched in low-dose, and Turicibacter was enriched in both BBR doses.
32231564	3	45	theme	high-fat	586:593	arg1	diet					595:598	a high-fat diet	584:598	a high-fat diet	584:598	The purposes of this study were to observe the effects of high or low doses of BBR on atherosclerosis and gut microbiota modulation, and to explore their correlation in ApoE-/- mice fed a high-fat diet.
32231564	11	46	theme	Metagenomic	1864:1874	arg1	analysis					1876:1883	Metagenomic analysis	1864:1883	Metagenomic analysis	1864:1883	Metagenomic analysis further showed an elevated potential for lipid and glycan metabolism and synthesis of SCFAs, as well as reduced potential of TMAO production after BBR treatment.
32231564	0	47	theme	Atherosclerosis	23:37	arg1	Modulation					58:67	Atherosclerosis and Gut Microbiota Modulation	23:67	Atherosclerosis and Gut Microbiota Modulation	23:67	Effect of Berberine on Atherosclerosis and Gut Microbiota Modulation and Their Correlation in High-Fat Diet-Fed ApoE-/- Mice.
32231564	14	48	theme	lipid	2485:2489	arg1	anti-inflammatory					2437:2453	anti-inflammatory	2437:2453	anti-inflammatory	2437:2453	This anti-atherosclerotic effect of BBR may be partly attributed to changes in composition and functions of gut microbiota which may be associated with anti-inflammatory and metabolism of glucose and lipid.
32231564	5	49	theme	high-dose	961:969	arg1	BBR					971:973	high-dose BBR	961:973	high-dose BBR	961:973	Both BBR treatments significantly reduced total cholesterol, APOB100, and very low-density lipoprotein cholesterol levels but levels of high/low-density lipoprotein cholesterol and lipoprotein (a) were only reduced by high-dose BBR.
32231564	6	50	theme	Decreased	976:984	arg1	cytokines					1003:1011	Decreased pro-inflammatory cytokines	976:1011	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels	976:1127	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	6	50	theme	Decreased	976:984	arg1	factor-alpha					1028:1039	tumor necrosis factor-alpha	1013:1039	tumor necrosis factor-alpha	1013:1039	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	6	50	theme	Decreased	976:984	arg1	IL-6					1063:1066	IL-6	1063:1066	IL-6	1063:1066	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	6	50	theme	Decreased	976:984	arg1	IL-10					1100:1104	increased anti-inflammatory IL-10	1072:1104	increased anti-inflammatory IL-10	1072:1104	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	6	50	theme	Decreased	976:984	arg1	IL					1055:1056	interleukin (IL)-1β	1042:1060	interleukin (IL)-1β	1042:1060	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	5	51	dep	cholesterol	791:801	arg1	cholesterol					846:856	low-density lipoprotein cholesterol	822:856	low-density lipoprotein cholesterol	822:856	Both BBR treatments significantly reduced total cholesterol, APOB100, and very low-density lipoprotein cholesterol levels but levels of high/low-density lipoprotein cholesterol and lipoprotein (a) were only reduced by high-dose BBR.
32231564	0	52	theme	Gut	43:45	arg1	Modulation					58:67	Atherosclerosis and Gut Microbiota Modulation	23:67	Atherosclerosis and Gut Microbiota Modulation	23:67	Effect of Berberine on Atherosclerosis and Gut Microbiota Modulation and Their Correlation in High-Fat Diet-Fed ApoE-/- Mice.
32231564	14	53	theme	glucose	2473:2479	arg1	anti-inflammatory					2437:2453	anti-inflammatory	2437:2453	anti-inflammatory	2437:2453	This anti-atherosclerotic effect of BBR may be partly attributed to changes in composition and functions of gut microbiota which may be associated with anti-inflammatory and metabolism of glucose and lipid.
32231564	9	54	theme	anti-inflammatory	1548:1564	arg1	effects					1566:1572	good anti-inflammatory effects	1543:1572	good anti-inflammatory effects related to the production of short-chain fatty acids (SCFAs)	1543:1633	These microbiota displayed good anti-inflammatory effects related to the production of short-chain fatty acids (SCFAs) and were related to glucolipid metabolism.
32231564	4	55	theme	dose	718:721	arg1	effect					699:704	the effect	695:704	the effect of the high dose	695:721	A significant decrease in atherosclerotic lesions was observed after treatment with BBR, with the effect of the high dose being more obvious.
32231564	4	55	theme	dose	718:721	arg1	obvious					734:740	obvious	734:740	obvious	734:740	A significant decrease in atherosclerotic lesions was observed after treatment with BBR, with the effect of the high dose being more obvious.
32231564	9	56	theme	related	1574:1580	arg1	effects					1566:1572	good anti-inflammatory effects	1543:1572	good anti-inflammatory effects related to the production of short-chain fatty acids (SCFAs)	1543:1633	These microbiota displayed good anti-inflammatory effects related to the production of short-chain fatty acids (SCFAs) and were related to glucolipid metabolism.
32231564	3	57	theme	low	464:466	arg1	doses					468:472	high or low doses	456:472	high or low doses of BBR	456:479	The purposes of this study were to observe the effects of high or low doses of BBR on atherosclerosis and gut microbiota modulation, and to explore their correlation in ApoE-/- mice fed a high-fat diet.
32231564	15	58	theme	different	2535:2543	arg1	sensitivity					2545:2555	different sensitivity	2535:2555	different sensitivity to BBR dose	2535:2567	Notably, gut microbiota alterations showed different sensitivity to BBR dose.
32231564	3	59	theme	high	456:459	arg1	doses					468:472	high or low doses	456:472	high or low doses of BBR	456:479	The purposes of this study were to observe the effects of high or low doses of BBR on atherosclerosis and gut microbiota modulation, and to explore their correlation in ApoE-/- mice fed a high-fat diet.
32231564	12	60	theme	systemic	2132:2139	arg1	inflammation					2141:2152	systemic inflammation	2132:2152	systemic inflammation	2132:2152	The findings demonstrate that both high and low-dose BBR can improve serum lipid and systemic inflammation levels, and alleviate atherosclerosis induced by high-fat diet in ApoE-/- mice.
32231564	4	61	from	decrease	615:622	arg1	lesions					643:649	atherosclerotic lesions	627:649	atherosclerotic lesions	627:649	A significant decrease in atherosclerotic lesions was observed after treatment with BBR, with the effect of the high dose being more obvious.
32231564	6	62	from	increase	1198:1205	arg1	IL-10					1210:1214	IL-10	1210:1214	IL-10	1210:1214	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	6	62	from	increase	1198:1205	arg1	IL-6					1190:1193	IL-6	1190:1193	IL-6	1190:1193	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	3	63	theme	microbiota	508:517	arg1	modulation					519:528	gut microbiota modulation	504:528	gut microbiota modulation	504:528	The purposes of this study were to observe the effects of high or low doses of BBR on atherosclerosis and gut microbiota modulation, and to explore their correlation in ApoE-/- mice fed a high-fat diet.
32231564	9	64	theme	fatty	1615:1619	arg1	SCFAs					1628:1632	SCFAs	1628:1632	SCFAs	1628:1632	These microbiota displayed good anti-inflammatory effects related to the production of short-chain fatty acids (SCFAs) and were related to glucolipid metabolism.
32231564	9	64	theme	fatty	1615:1619	arg1	acids					1621:1625	short-chain fatty acids	1603:1625	short-chain fatty acids (SCFAs)	1603:1633	These microbiota displayed good anti-inflammatory effects related to the production of short-chain fatty acids (SCFAs) and were related to glucolipid metabolism.
32231564	5	65	theme	low-density	822:832	arg1	cholesterol					846:856	low-density lipoprotein cholesterol	822:856	low-density lipoprotein cholesterol	822:856	Both BBR treatments significantly reduced total cholesterol, APOB100, and very low-density lipoprotein cholesterol levels but levels of high/low-density lipoprotein cholesterol and lipoprotein (a) were only reduced by high-dose BBR.
32231564	14	66	theme	BBR	2321:2323	arg1	effect					2311:2316	This anti-atherosclerotic effect	2285:2316	This anti-atherosclerotic effect of BBR	2285:2323	This anti-atherosclerotic effect of BBR may be partly attributed to changes in composition and functions of gut microbiota which may be associated with anti-inflammatory and metabolism of glucose and lipid.
32231564	5	67	theme	cholesterol	791:801	arg1	levels					858:863	total cholesterol, APOB100, and very low-density lipoprotein cholesterol levels	785:863	total cholesterol, APOB100, and very low-density lipoprotein cholesterol levels	785:863	Both BBR treatments significantly reduced total cholesterol, APOB100, and very low-density lipoprotein cholesterol levels but levels of high/low-density lipoprotein cholesterol and lipoprotein (a) were only reduced by high-dose BBR.
32231564	14	68	theme	microbiota	2397:2406	arg1	functions					2380:2388	functions	2380:2388	functions	2380:2388	This anti-atherosclerotic effect of BBR may be partly attributed to changes in composition and functions of gut microbiota which may be associated with anti-inflammatory and metabolism of glucose and lipid.
32231564	14	68	theme	microbiota	2397:2406	arg1	composition					2364:2374	composition	2364:2374	composition	2364:2374	This anti-atherosclerotic effect of BBR may be partly attributed to changes in composition and functions of gut microbiota which may be associated with anti-inflammatory and metabolism of glucose and lipid.
32231564	10	69	theme	BBR	1743:1745	arg1	group					1747:1751	high-dose BBR group	1733:1751	high-dose BBR group	1733:1751	Alistipes and Roseburia were significantly enriched in high-dose BBR group while Blautia and Allobaculum were more enriched in low-dose, and Turicibacter was enriched in both BBR doses.
32231564	5	70	theme	APOB100	804:810	arg1	levels					858:863	total cholesterol, APOB100, and very low-density lipoprotein cholesterol levels	785:863	total cholesterol, APOB100, and very low-density lipoprotein cholesterol levels	785:863	Both BBR treatments significantly reduced total cholesterol, APOB100, and very low-density lipoprotein cholesterol levels but levels of high/low-density lipoprotein cholesterol and lipoprotein (a) were only reduced by high-dose BBR.
32231564	7	71	theme	gut	1354:1356	arg1	microbiota					1358:1367	gut microbiota	1354:1367	gut microbiota	1354:1367	16S rRNA sequencing showed that BBR significantly altered the community compositional structure of gut microbiota.
32231564	12	72	theme	high	2082:2085	arg1	BBR					2100:2102	both high and low-dose BBR	2077:2102	both high and low-dose BBR	2077:2102	The findings demonstrate that both high and low-dose BBR can improve serum lipid and systemic inflammation levels, and alleviate atherosclerosis induced by high-fat diet in ApoE-/- mice.
32231564	14	73	theme	anti-atherosclerotic	2290:2309	arg1	effect					2311:2316	This anti-atherosclerotic effect	2285:2316	This anti-atherosclerotic effect of BBR	2285:2323	This anti-atherosclerotic effect of BBR may be partly attributed to changes in composition and functions of gut microbiota which may be associated with anti-inflammatory and metabolism of glucose and lipid.
32231564	1	74	theme	serious	196:202	arg1	Atherosclerosis					126:140	Atherosclerosis	126:140	Atherosclerosis	126:140	Atherosclerosis and its associated cardiovascular diseases (CVDs) are serious threats to human health and have been reported to be associated with the gut microbiota.
32231564	1	74	theme	serious	196:202	arg1	threats					204:210	serious threats	196:210	serious threats to human health	196:226	Atherosclerosis and its associated cardiovascular diseases (CVDs) are serious threats to human health and have been reported to be associated with the gut microbiota.
32231564	1	74	theme	serious	196:202	arg1	diseases					176:183	its associated cardiovascular diseases	146:183	its associated cardiovascular diseases (CVDs)	146:190	Atherosclerosis and its associated cardiovascular diseases (CVDs) are serious threats to human health and have been reported to be associated with the gut microbiota.
32231564	11	75	theme	TMAO	2010:2013	arg1	production					2015:2024	TMAO production	2010:2024	TMAO production	2010:2024	Metagenomic analysis further showed an elevated potential for lipid and glycan metabolism and synthesis of SCFAs, as well as reduced potential of TMAO production after BBR treatment.
32231564	0	76	from	Correlation	79:89	arg1	Mice					120:123	High-Fat Diet-Fed ApoE-/- Mice	94:123	High-Fat Diet-Fed ApoE-/- Mice	94:123	Effect of Berberine on Atherosclerosis and Gut Microbiota Modulation and Their Correlation in High-Fat Diet-Fed ApoE-/- Mice.
32231564	6	77	theme	anti-inflammatory	1082:1098	arg1	cytokines					1003:1011	Decreased pro-inflammatory cytokines	976:1011	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels	976:1127	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	6	77	theme	anti-inflammatory	1082:1098	arg1	IL-10					1100:1104	increased anti-inflammatory IL-10	1072:1104	increased anti-inflammatory IL-10	1072:1104	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	12	78	dep	lipid	2122:2126	arg1	levels					2154:2159	levels	2154:2159	levels	2154:2159	The findings demonstrate that both high and low-dose BBR can improve serum lipid and systemic inflammation levels, and alleviate atherosclerosis induced by high-fat diet in ApoE-/- mice.
32231564	7	79	theme	community	1317:1325	arg1	structure					1341:1349	the community compositional structure	1313:1349	the community compositional structure of gut microbiota	1313:1367	16S rRNA sequencing showed that BBR significantly altered the community compositional structure of gut microbiota.
32231564	11	80	theme	glycan	1936:1941	arg1	metabolism					1943:1952	lipid and glycan metabolism	1926:1952	metabolism	1943:1952	Metagenomic analysis further showed an elevated potential for lipid and glycan metabolism and synthesis of SCFAs, as well as reduced potential of TMAO production after BBR treatment.
32231564	8	81	theme	Blautia	1425:1431	arg1	abundance					1401:1409	the abundance	1397:1409	the abundance of Roseburia, Blautia, Allobaculum, Alistipes, and Turicibacter	1397:1473	Specifically, BBR enriched the abundance of Roseburia, Blautia, Allobaculum, Alistipes, and Turicibacter, and changed the abundance of Bilophila.
32231564	6	82	theme	BBR	1160:1162	arg1	group					1164:1168	the high-dose BBR group	1146:1168	the high-dose BBR group	1146:1168	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	6	83	from	decrease	1178:1185	arg1	IL-10					1210:1214	IL-10	1210:1214	IL-10	1210:1214	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	6	83	from	decrease	1178:1185	arg1	IL-6					1190:1193	IL-6	1190:1193	IL-6	1190:1193	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	11	84	theme	lipid	1926:1930	arg1	metabolism					1943:1952	lipid and glycan metabolism	1926:1952	metabolism	1943:1952	Metagenomic analysis further showed an elevated potential for lipid and glycan metabolism and synthesis of SCFAs, as well as reduced potential of TMAO production after BBR treatment.
32231564	14	85	from	changes	2353:2359	arg1	functions					2380:2388	functions	2380:2388	functions	2380:2388	This anti-atherosclerotic effect of BBR may be partly attributed to changes in composition and functions of gut microbiota which may be associated with anti-inflammatory and metabolism of glucose and lipid.
32231564	14	85	from	changes	2353:2359	arg1	composition					2364:2374	composition	2364:2374	composition	2364:2374	This anti-atherosclerotic effect of BBR may be partly attributed to changes in composition and functions of gut microbiota which may be associated with anti-inflammatory and metabolism of glucose and lipid.
32231564	1	86	theme	cardiovascular	161:174	arg1	Atherosclerosis					126:140	Atherosclerosis	126:140	Atherosclerosis	126:140	Atherosclerosis and its associated cardiovascular diseases (CVDs) are serious threats to human health and have been reported to be associated with the gut microbiota.
32231564	1	86	theme	cardiovascular	161:174	arg1	threats					204:210	serious threats	196:210	serious threats to human health	196:226	Atherosclerosis and its associated cardiovascular diseases (CVDs) are serious threats to human health and have been reported to be associated with the gut microbiota.
32231564	1	86	theme	cardiovascular	161:174	arg1	CVDs					186:189	CVDs	186:189	CVDs	186:189	Atherosclerosis and its associated cardiovascular diseases (CVDs) are serious threats to human health and have been reported to be associated with the gut microbiota.
32231564	1	86	theme	cardiovascular	161:174	arg1	diseases					176:183	its associated cardiovascular diseases	146:183	its associated cardiovascular diseases (CVDs)	146:190	Atherosclerosis and its associated cardiovascular diseases (CVDs) are serious threats to human health and have been reported to be associated with the gut microbiota.
32231564	8	87	theme	Alistipes	1447:1455	arg1	abundance					1401:1409	the abundance	1397:1409	the abundance of Roseburia, Blautia, Allobaculum, Alistipes, and Turicibacter	1397:1473	Specifically, BBR enriched the abundance of Roseburia, Blautia, Allobaculum, Alistipes, and Turicibacter, and changed the abundance of Bilophila.
32231564	4	88	theme	atherosclerotic	627:641	arg1	lesions					643:649	atherosclerotic lesions	627:649	atherosclerotic lesions	627:649	A significant decrease in atherosclerotic lesions was observed after treatment with BBR, with the effect of the high dose being more obvious.
32231564	0	89	theme	High-Fat	94:101	arg1	Mice					120:123	High-Fat Diet-Fed ApoE-/- Mice	94:123	High-Fat Diet-Fed ApoE-/- Mice	94:123	Effect of Berberine on Atherosclerosis and Gut Microbiota Modulation and Their Correlation in High-Fat Diet-Fed ApoE-/- Mice.
32231564	12	90	theme	low-dose	2091:2098	arg1	BBR					2100:2102	both high and low-dose BBR	2077:2102	both high and low-dose BBR	2077:2102	The findings demonstrate that both high and low-dose BBR can improve serum lipid and systemic inflammation levels, and alleviate atherosclerosis induced by high-fat diet in ApoE-/- mice.
32231564	0	91	theme	ApoE-/-	112:118	arg1	Mice					120:123	High-Fat Diet-Fed ApoE-/- Mice	94:123	High-Fat Diet-Fed ApoE-/- Mice	94:123	Effect of Berberine on Atherosclerosis and Gut Microbiota Modulation and Their Correlation in High-Fat Diet-Fed ApoE-/- Mice.
32231564	6	92	theme	interleukin	1042:1052	arg1	cytokines					1003:1011	Decreased pro-inflammatory cytokines	976:1011	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels	976:1127	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	6	92	theme	interleukin	1042:1052	arg1	IL					1055:1056	interleukin (IL)-1β	1042:1060	interleukin (IL)-1β	1042:1060	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	12	93	theme	ApoE-/-	2220:2226	arg1	mice					2228:2231	ApoE-/- mice	2220:2231	ApoE-/- mice	2220:2231	The findings demonstrate that both high and low-dose BBR can improve serum lipid and systemic inflammation levels, and alleviate atherosclerosis induced by high-fat diet in ApoE-/- mice.
32231564	6	94	dep	cytokines	1003:1011	arg1	cytokines					1003:1011	Decreased pro-inflammatory cytokines	976:1011	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels	976:1127	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	6	94	dep	cytokines	1003:1011	arg1	factor-alpha					1028:1039	tumor necrosis factor-alpha	1013:1039	tumor necrosis factor-alpha	1013:1039	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	6	94	dep	cytokines	1003:1011	arg1	IL					1055:1056	interleukin (IL)-1β	1042:1060	interleukin (IL)-1β	1042:1060	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	6	94	dep	cytokines	1003:1011	arg1	levels					1122:1127	adiponectin levels	1110:1127	adiponectin levels	1110:1127	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	6	94	dep	cytokines	1003:1011	arg1	IL-6					1063:1066	IL-6	1063:1066	IL-6	1063:1066	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	12	95	from	diet	2212:2215	arg1	mice					2228:2231	ApoE-/- mice	2220:2231	ApoE-/- mice	2220:2231	The findings demonstrate that both high and low-dose BBR can improve serum lipid and systemic inflammation levels, and alleviate atherosclerosis induced by high-fat diet in ApoE-/- mice.
32231564	5	96	theme	lipoprotein	896:906	arg1	cholesterol					908:918	high/low-density lipoprotein cholesterol	879:918	high/low-density lipoprotein cholesterol	879:918	Both BBR treatments significantly reduced total cholesterol, APOB100, and very low-density lipoprotein cholesterol levels but levels of high/low-density lipoprotein cholesterol and lipoprotein (a) were only reduced by high-dose BBR.
32231564	6	97	theme	tumor	1013:1017	arg1	cytokines					1003:1011	Decreased pro-inflammatory cytokines	976:1011	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels	976:1127	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	6	97	theme	tumor	1013:1017	arg1	factor-alpha					1028:1039	tumor necrosis factor-alpha	1013:1039	tumor necrosis factor-alpha	1013:1039	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	6	98	theme	pro-inflammatory	986:1001	arg1	cytokines					1003:1011	Decreased pro-inflammatory cytokines	976:1011	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels	976:1127	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	6	98	theme	pro-inflammatory	986:1001	arg1	factor-alpha					1028:1039	tumor necrosis factor-alpha	1013:1039	tumor necrosis factor-alpha	1013:1039	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	6	98	theme	pro-inflammatory	986:1001	arg1	IL-6					1063:1066	IL-6	1063:1066	IL-6	1063:1066	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	6	98	theme	pro-inflammatory	986:1001	arg1	IL-10					1100:1104	increased anti-inflammatory IL-10	1072:1104	increased anti-inflammatory IL-10	1072:1104	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	6	98	theme	pro-inflammatory	986:1001	arg1	IL					1055:1056	interleukin (IL)-1β	1042:1060	interleukin (IL)-1β	1042:1060	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	7	99	theme	16S	1255:1257	arg1	sequencing					1264:1273	16S rRNA sequencing	1255:1273	16S rRNA sequencing	1255:1273	16S rRNA sequencing showed that BBR significantly altered the community compositional structure of gut microbiota.
32231564	8	100	theme	Bilophila	1505:1513	arg1	abundance					1492:1500	the abundance	1488:1500	the abundance of Bilophila	1488:1513	Specifically, BBR enriched the abundance of Roseburia, Blautia, Allobaculum, Alistipes, and Turicibacter, and changed the abundance of Bilophila.
32231564	12	101	theme	serum	2116:2120	arg1	lipid					2122:2126	serum lipid	2116:2126	serum lipid	2116:2126	The findings demonstrate that both high and low-dose BBR can improve serum lipid and systemic inflammation levels, and alleviate atherosclerosis induced by high-fat diet in ApoE-/- mice.
32231564	3	102	theme	study	419:423	arg1	purposes					402:409	The purposes	398:409	The purposes of this study	398:423	The purposes of this study were to observe the effects of high or low doses of BBR on atherosclerosis and gut microbiota modulation, and to explore their correlation in ApoE-/- mice fed a high-fat diet.
32231564	0	103	theme	Microbiota	47:56	arg1	Modulation					58:67	Atherosclerosis and Gut Microbiota Modulation	23:67	Atherosclerosis and Gut Microbiota Modulation	23:67	Effect of Berberine on Atherosclerosis and Gut Microbiota Modulation and Their Correlation in High-Fat Diet-Fed ApoE-/- Mice.
32231564	9	104	theme	good	1543:1546	arg1	effects					1566:1572	good anti-inflammatory effects	1543:1572	good anti-inflammatory effects related to the production of short-chain fatty acids (SCFAs)	1543:1633	These microbiota displayed good anti-inflammatory effects related to the production of short-chain fatty acids (SCFAs) and were related to glucolipid metabolism.
32231564	6	105	theme	increased	1072:1080	arg1	cytokines					1003:1011	Decreased pro-inflammatory cytokines	976:1011	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels	976:1127	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	6	105	theme	increased	1072:1080	arg1	IL-10					1100:1104	increased anti-inflammatory IL-10	1072:1104	increased anti-inflammatory IL-10	1072:1104	Decreased pro-inflammatory cytokines tumor necrosis factor-alpha, interleukin (IL)-1β, IL-6 and increased anti-inflammatory IL-10 and adiponectin levels were observed in the high-dose BBR group, but no decrease in IL-6 or increase in IL-10 was evident using the low-dose of BBR.
32231564	2	106	theme	berberine	315:323	arg1	role					307:310	the role	303:310	the role of berberine (BBR) in atherosclerosis and gut microbiota	303:367	Recently, the role of berberine (BBR) in atherosclerosis and gut microbiota has begun to be appreciated.
32231564	4	107	theme	high	713:716	arg1	dose					718:721	the high dose	709:721	the high dose	709:721	A significant decrease in atherosclerotic lesions was observed after treatment with BBR, with the effect of the high dose being more obvious.
32231564	10	108	from	low-dose	1805:1812	arg1	enriched					1793:1800	enriched	1793:1800	enriched	1793:1800	Alistipes and Roseburia were significantly enriched in high-dose BBR group while Blautia and Allobaculum were more enriched in low-dose, and Turicibacter was enriched in both BBR doses.
32231564	1	109	theme	human	215:219	arg1	health					221:226	human health	215:226	human health	215:226	Atherosclerosis and its associated cardiovascular diseases (CVDs) are serious threats to human health and have been reported to be associated with the gut microbiota.
32231564	4	110	with	treatment	670:678	arg1	BBR					685:687	BBR	685:687	BBR	685:687	A significant decrease in atherosclerotic lesions was observed after treatment with BBR, with the effect of the high dose being more obvious.
32231564	3	111	theme	doses	468:472	arg1	effects					445:451	the effects	441:451	the effects of high or low doses of BBR on atherosclerosis and gut microbiota modulation	441:528	The purposes of this study were to observe the effects of high or low doses of BBR on atherosclerosis and gut microbiota modulation, and to explore their correlation in ApoE-/- mice fed a high-fat diet.
32231564	15	112	theme	microbiota	2505:2514	arg1	alterations					2516:2526	gut microbiota alterations	2501:2526	gut microbiota alterations	2501:2526	Notably, gut microbiota alterations showed different sensitivity to BBR dose.
32294957	4	0	theme	anti-inflammatory	1039:1055	arg1	activities					1057:1066	the antioxidant and anti-inflammatory activities	1019:1066	the antioxidant and anti-inflammatory activities of the extracts	1019:1082	In vitro enzyme inhibitory assays and in vivo assays were used to investigate the antioxidant and anti-inflammatory activities of the extracts.
32294957	4	1	theme	antioxidant	1023:1033	arg1	activities					1057:1066	the antioxidant and anti-inflammatory activities	1019:1066	the antioxidant and anti-inflammatory activities of the extracts	1019:1082	In vitro enzyme inhibitory assays and in vivo assays were used to investigate the antioxidant and anti-inflammatory activities of the extracts.
32294957	3	2	theme	anti-inflammatory	768:784	arg1	potential					786:794	the anti-inflammatory potential	764:794	the anti-inflammatory potential	764:794	Molecular docking study was carried out to estimate the anti-inflammatory potential and the binding affinities of some individual secondary metabolites from both extracts to the main enzymes involved in the inflammation pathway.
32294957	5	3	theme	analgesic	1177:1185	arg1	effects					1204:1210	antioxidant, anti-inflammatory, analgesic, and antipyretic effects	1145:1210	antioxidant, anti-inflammatory, analgesic, and antipyretic effects	1145:1210	Results revealed that both studied Thymus species exhibited antioxidant, anti-inflammatory, analgesic, and antipyretic effects.
32294957	8	4	theme	disorders	1603:1611	arg1	treatment					1548:1556	the treatment	1544:1556	the treatment of inflammation and oxidative stress-related disorders	1544:1611	In conclusion, Thymus algeriensis and Thymus fontanesii may be interesting candidates for the treatment of inflammation and oxidative stress-related disorders.
32294957	4	5	theme	inhibitory	957:966	arg1	assays					968:973	In vitro enzyme inhibitory assays	941:973	In vitro enzyme inhibitory assays	941:973	In vitro enzyme inhibitory assays and in vivo assays were used to investigate the antioxidant and anti-inflammatory activities of the extracts.
32294957	3	6	theme	Molecular	712:720	arg1	study					730:734	Molecular docking study	712:734	Molecular docking study	712:734	Molecular docking study was carried out to estimate the anti-inflammatory potential and the binding affinities of some individual secondary metabolites from both extracts to the main enzymes involved in the inflammation pathway.
32294957	2	7	theme	diterpene	616:624	arg1	carnosol					626:633	the phenolic diterpene carnosol	603:633	the phenolic diterpene carnosol	603:633	Thirty-five secondary metabolites were characterized in both extracts using HPLC-PDA-ESI-MS/MS. Phenolic acids, mainly rosmarinic acid and its derivatives, dominated the T. algeriensis extract, while the phenolic diterpene carnosol and the methylated flavonoid salvigenin, prevailed in T. fontanesii extract.
32294957	3	8	from	affinities	812:821	arg1	extracts					874:881	both extracts	869:881	both extracts	869:881	Molecular docking study was carried out to estimate the anti-inflammatory potential and the binding affinities of some individual secondary metabolites from both extracts to the main enzymes involved in the inflammation pathway.
32294957	5	9	theme	Thymus	1120:1125	arg1	species					1127:1133	both studied Thymus species	1107:1133	both studied Thymus species	1107:1133	Results revealed that both studied Thymus species exhibited antioxidant, anti-inflammatory, analgesic, and antipyretic effects.
32294957	0	10	theme	Pain	90:93	arg1	Killing					95:101	Pain Killing	90:101	Pain Killing	90:101	Thymus algeriensis and Thymus fontanesii: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison.
32294957	3	11	theme	docking	722:728	arg1	study					730:734	Molecular docking study	712:734	Molecular docking study	712:734	Molecular docking study was carried out to estimate the anti-inflammatory potential and the binding affinities of some individual secondary metabolites from both extracts to the main enzymes involved in the inflammation pathway.
32294957	1	12	from	properties	298:307	arg1	the					335:337	the	335:337	the	335:337	This study aimed to investigate the chemical composition, and evaluate the antioxidant, anti-inflammatory, anti-pyretic, and the analgesic properties of methanol extracts from the leaves of Thymus algeriensis and Thymus fontanesii (Lamiaceae).
32294957	6	13	theme	potent	1238:1243	arg1	antioxidant					1245:1255	a more potent antioxidant	1231:1255	a more potent antioxidant than ascorbic acid	1231:1274	They showed to be a more potent antioxidant than ascorbic acid and more selective against cyclooxygenase (COX-2) than diclofenac and indomethacin.
32294957	1	14	theme	Thymus	349:354	arg1	algeriensis					356:366	Thymus algeriensis	349:366	Thymus algeriensis	349:366	This study aimed to investigate the chemical composition, and evaluate the antioxidant, anti-inflammatory, anti-pyretic, and the analgesic properties of methanol extracts from the leaves of Thymus algeriensis and Thymus fontanesii (Lamiaceae).
32294957	5	15	theme	anti-inflammatory	1158:1174	arg1	effects					1204:1210	antioxidant, anti-inflammatory, analgesic, and antipyretic effects	1145:1210	antioxidant, anti-inflammatory, analgesic, and antipyretic effects	1145:1210	Results revealed that both studied Thymus species exhibited antioxidant, anti-inflammatory, analgesic, and antipyretic effects.
32294957	2	16	theme	flavonoid	654:662	arg1	salvigenin					664:673	the methylated flavonoid salvigenin	639:673	the methylated flavonoid salvigenin	639:673	Thirty-five secondary metabolites were characterized in both extracts using HPLC-PDA-ESI-MS/MS. Phenolic acids, mainly rosmarinic acid and its derivatives, dominated the T. algeriensis extract, while the phenolic diterpene carnosol and the methylated flavonoid salvigenin, prevailed in T. fontanesii extract.
32294957	2	17	theme	secondary	415:423	arg1	metabolites					425:435	Thirty-five secondary metabolites	403:435	Thirty-five secondary metabolites	403:435	Thirty-five secondary metabolites were characterized in both extracts using HPLC-PDA-ESI-MS/MS. Phenolic acids, mainly rosmarinic acid and its derivatives, dominated the T. algeriensis extract, while the phenolic diterpene carnosol and the methylated flavonoid salvigenin, prevailed in T. fontanesii extract.
32294957	1	18	from	the	335:337	arg1	properties					298:307	the analgesic properties	284:307	the analgesic properties of methanol extracts from the leaves of Thymus algeriensis and Thymus fontanesii (Lamiaceae)	284:400	This study aimed to investigate the chemical composition, and evaluate the antioxidant, anti-inflammatory, anti-pyretic, and the analgesic properties of methanol extracts from the leaves of Thymus algeriensis and Thymus fontanesii (Lamiaceae).
32294957	1	18	from	the	335:337	arg1	extracts					321:328	methanol extracts	312:328	methanol extracts from the leaves of Thymus algeriensis and Thymus fontanesii (Lamiaceae)	312:400	This study aimed to investigate the chemical composition, and evaluate the antioxidant, anti-inflammatory, anti-pyretic, and the analgesic properties of methanol extracts from the leaves of Thymus algeriensis and Thymus fontanesii (Lamiaceae).
32294957	2	19	theme	methylated	643:652	arg1	salvigenin					664:673	the methylated flavonoid salvigenin	639:673	the methylated flavonoid salvigenin	639:673	Thirty-five secondary metabolites were characterized in both extracts using HPLC-PDA-ESI-MS/MS. Phenolic acids, mainly rosmarinic acid and its derivatives, dominated the T. algeriensis extract, while the phenolic diterpene carnosol and the methylated flavonoid salvigenin, prevailed in T. fontanesii extract.
32294957	8	20	theme	oxidative	1578:1586	arg1	disorders					1603:1611	oxidative stress-related disorders	1578:1611	oxidative stress-related disorders	1578:1611	In conclusion, Thymus algeriensis and Thymus fontanesii may be interesting candidates for the treatment of inflammation and oxidative stress-related disorders.
32294957	7	21	theme	COX-2	1417:1421	arg1	inhibitor					1423:1431	COX-2 inhibitor	1417:1431	COX-2 inhibitor	1417:1431	Relatively, the T. fontanesii extract was more potent as COX-2 inhibitor than T. algeriensis.
32294957	4	22	theme	in	979:980	arg1	assays					987:992	in vivo assays	979:992	in vivo assays	979:992	In vitro enzyme inhibitory assays and in vivo assays were used to investigate the antioxidant and anti-inflammatory activities of the extracts.
32294957	4	23	theme	extracts	1075:1082	arg1	activities					1057:1066	the antioxidant and anti-inflammatory activities	1019:1066	the antioxidant and anti-inflammatory activities of the extracts	1019:1082	In vitro enzyme inhibitory assays and in vivo assays were used to investigate the antioxidant and anti-inflammatory activities of the extracts.
32294957	1	24	theme	Thymus	372:377	arg1	Lamiaceae					391:399	Lamiaceae	391:399	Lamiaceae	391:399	This study aimed to investigate the chemical composition, and evaluate the antioxidant, anti-inflammatory, anti-pyretic, and the analgesic properties of methanol extracts from the leaves of Thymus algeriensis and Thymus fontanesii (Lamiaceae).
32294957	1	24	theme	Thymus	372:377	arg1	fontanesii					379:388	Thymus fontanesii	372:388	Thymus fontanesii (Lamiaceae)	372:400	This study aimed to investigate the chemical composition, and evaluate the antioxidant, anti-inflammatory, anti-pyretic, and the analgesic properties of methanol extracts from the leaves of Thymus algeriensis and Thymus fontanesii (Lamiaceae).
32294957	3	25	from	potential	786:794	arg1	extracts					874:881	both extracts	869:881	both extracts	869:881	Molecular docking study was carried out to estimate the anti-inflammatory potential and the binding affinities of some individual secondary metabolites from both extracts to the main enzymes involved in the inflammation pathway.
32294957	2	26	theme	rosmarinic	522:531	arg1	acids					508:512	HPLC-PDA-ESI-MS/MS. Phenolic acids	479:512	HPLC-PDA-ESI-MS/MS. Phenolic acids	479:512	Thirty-five secondary metabolites were characterized in both extracts using HPLC-PDA-ESI-MS/MS. Phenolic acids, mainly rosmarinic acid and its derivatives, dominated the T. algeriensis extract, while the phenolic diterpene carnosol and the methylated flavonoid salvigenin, prevailed in T. fontanesii extract.
32294957	2	26	theme	rosmarinic	522:531	arg1	acid					533:536	rosmarinic acid	522:536	rosmarinic acid	522:536	Thirty-five secondary metabolites were characterized in both extracts using HPLC-PDA-ESI-MS/MS. Phenolic acids, mainly rosmarinic acid and its derivatives, dominated the T. algeriensis extract, while the phenolic diterpene carnosol and the methylated flavonoid salvigenin, prevailed in T. fontanesii extract.
32294957	1	27	dep	the	335:337	arg1	leaves					339:344	leaves	339:344	leaves	339:344	This study aimed to investigate the chemical composition, and evaluate the antioxidant, anti-inflammatory, anti-pyretic, and the analgesic properties of methanol extracts from the leaves of Thymus algeriensis and Thymus fontanesii (Lamiaceae).
32294957	8	28	theme	stress-related	1588:1601	arg1	disorders					1603:1611	oxidative stress-related disorders	1578:1611	oxidative stress-related disorders	1578:1611	In conclusion, Thymus algeriensis and Thymus fontanesii may be interesting candidates for the treatment of inflammation and oxidative stress-related disorders.
32294957	7	29	theme	T.	1376:1377	arg1	extract					1390:1396	the T. fontanesii extract	1372:1396	the T. fontanesii extract	1372:1396	Relatively, the T. fontanesii extract was more potent as COX-2 inhibitor than T. algeriensis.
32294957	7	29	theme	T.	1376:1377	arg1	potent					1407:1412	potent	1407:1412	potent	1407:1412	Relatively, the T. fontanesii extract was more potent as COX-2 inhibitor than T. algeriensis.
32294957	0	30	theme	Thymus	0:5	arg1	algeriensis					7:17	Thymus algeriensis	0:17	Thymus algeriensis	0:17	Thymus algeriensis and Thymus fontanesii: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison.
32294957	2	31	theme	fontanesii	692:701	arg1	extract					703:709	T. fontanesii extract	689:709	T. fontanesii extract	689:709	Thirty-five secondary metabolites were characterized in both extracts using HPLC-PDA-ESI-MS/MS. Phenolic acids, mainly rosmarinic acid and its derivatives, dominated the T. algeriensis extract, while the phenolic diterpene carnosol and the methylated flavonoid salvigenin, prevailed in T. fontanesii extract.
32294957	8	32	theme	Thymus	1469:1474	arg1	algeriensis					1476:1486	Thymus algeriensis	1469:1486	Thymus algeriensis	1469:1486	In conclusion, Thymus algeriensis and Thymus fontanesii may be interesting candidates for the treatment of inflammation and oxidative stress-related disorders.
32294957	7	33	theme	fontanesii	1379:1388	arg1	extract					1390:1396	the T. fontanesii extract	1372:1396	the T. fontanesii extract	1372:1396	Relatively, the T. fontanesii extract was more potent as COX-2 inhibitor than T. algeriensis.
32294957	7	33	theme	fontanesii	1379:1388	arg1	potent					1407:1412	potent	1407:1412	potent	1407:1412	Relatively, the T. fontanesii extract was more potent as COX-2 inhibitor than T. algeriensis.
32294957	0	34	theme	Thymus	23:28	arg1	fontanesii					30:39	Thymus fontanesii	23:39	Thymus fontanesii	23:39	Thymus algeriensis and Thymus fontanesii: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison.
32294957	8	35	theme	Thymus	1492:1497	arg1	fontanesii					1499:1508	Thymus fontanesii	1492:1508	Thymus fontanesii	1492:1508	In conclusion, Thymus algeriensis and Thymus fontanesii may be interesting candidates for the treatment of inflammation and oxidative stress-related disorders.
32294957	2	36	theme	T.	573:574	arg1	extract					588:594	the T. algeriensis extract	569:594	the T. algeriensis extract	569:594	Thirty-five secondary metabolites were characterized in both extracts using HPLC-PDA-ESI-MS/MS. Phenolic acids, mainly rosmarinic acid and its derivatives, dominated the T. algeriensis extract, while the phenolic diterpene carnosol and the methylated flavonoid salvigenin, prevailed in T. fontanesii extract.
32294957	2	37	theme	T.	689:690	arg1	extract					703:709	T. fontanesii extract	689:709	T. fontanesii extract	689:709	Thirty-five secondary metabolites were characterized in both extracts using HPLC-PDA-ESI-MS/MS. Phenolic acids, mainly rosmarinic acid and its derivatives, dominated the T. algeriensis extract, while the phenolic diterpene carnosol and the methylated flavonoid salvigenin, prevailed in T. fontanesii extract.
32294957	0	38	dep	In	64:65	arg1	Vivo					67:70	Vivo	67:70	Vivo	67:70	Thymus algeriensis and Thymus fontanesii: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison.
32294957	0	38	dep	In	64:65	arg1	Antiinflammatory					72:87	Antiinflammatory	72:87	Antiinflammatory	72:87	Thymus algeriensis and Thymus fontanesii: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison.
32294957	8	39	from	candidates	1529:1538	arg1	conclusion					1457:1466	conclusion	1457:1466	conclusion	1457:1466	In conclusion, Thymus algeriensis and Thymus fontanesii may be interesting candidates for the treatment of inflammation and oxidative stress-related disorders.
32294957	0	40	theme	Antipyretic	107:117	arg1	Activities					119:128	Antipyretic Activities	107:128	Antipyretic Activities	107:128	Thymus algeriensis and Thymus fontanesii: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison.
32294957	4	41	dep	In	941:942	arg1	vitro					944:948	vitro	944:948	vitro	944:948	In vitro enzyme inhibitory assays and in vivo assays were used to investigate the antioxidant and anti-inflammatory activities of the extracts.
32294957	1	42	theme	chemical	195:202	arg1	composition					204:214	the chemical composition	191:214	the chemical composition	191:214	This study aimed to investigate the chemical composition, and evaluate the antioxidant, anti-inflammatory, anti-pyretic, and the analgesic properties of methanol extracts from the leaves of Thymus algeriensis and Thymus fontanesii (Lamiaceae).
32294957	2	43	theme	Phenolic	499:506	arg1	acids					508:512	HPLC-PDA-ESI-MS/MS. Phenolic acids	479:512	HPLC-PDA-ESI-MS/MS. Phenolic acids	479:512	Thirty-five secondary metabolites were characterized in both extracts using HPLC-PDA-ESI-MS/MS. Phenolic acids, mainly rosmarinic acid and its derivatives, dominated the T. algeriensis extract, while the phenolic diterpene carnosol and the methylated flavonoid salvigenin, prevailed in T. fontanesii extract.
32294957	2	43	theme	Phenolic	499:506	arg1	acid					533:536	rosmarinic acid	522:536	rosmarinic acid	522:536	Thirty-five secondary metabolites were characterized in both extracts using HPLC-PDA-ESI-MS/MS. Phenolic acids, mainly rosmarinic acid and its derivatives, dominated the T. algeriensis extract, while the phenolic diterpene carnosol and the methylated flavonoid salvigenin, prevailed in T. fontanesii extract.
32294957	2	43	theme	Phenolic	499:506	arg1	derivatives					546:556	its derivatives	542:556	its derivatives	542:556	Thirty-five secondary metabolites were characterized in both extracts using HPLC-PDA-ESI-MS/MS. Phenolic acids, mainly rosmarinic acid and its derivatives, dominated the T. algeriensis extract, while the phenolic diterpene carnosol and the methylated flavonoid salvigenin, prevailed in T. fontanesii extract.
32294957	1	44	theme	analgesic	288:296	arg1	properties					298:307	the analgesic properties	284:307	the analgesic properties of methanol extracts from the leaves of Thymus algeriensis and Thymus fontanesii (Lamiaceae)	284:400	This study aimed to investigate the chemical composition, and evaluate the antioxidant, anti-inflammatory, anti-pyretic, and the analgesic properties of methanol extracts from the leaves of Thymus algeriensis and Thymus fontanesii (Lamiaceae).
32294957	3	45	from	extracts	874:881	arg1	potential					786:794	the anti-inflammatory potential	764:794	the anti-inflammatory potential	764:794	Molecular docking study was carried out to estimate the anti-inflammatory potential and the binding affinities of some individual secondary metabolites from both extracts to the main enzymes involved in the inflammation pathway.
32294957	3	45	from	extracts	874:881	arg1	metabolites					852:862	some individual secondary metabolites	826:862	some individual secondary metabolites from both extracts	826:881	Molecular docking study was carried out to estimate the anti-inflammatory potential and the binding affinities of some individual secondary metabolites from both extracts to the main enzymes involved in the inflammation pathway.
32294957	3	45	from	extracts	874:881	arg1	affinities					812:821	the binding affinities	800:821	the binding affinities of some individual secondary metabolites from both extracts to the main enzymes involved in the inflammation pathway	800:938	Molecular docking study was carried out to estimate the anti-inflammatory potential and the binding affinities of some individual secondary metabolites from both extracts to the main enzymes involved in the inflammation pathway.
32294957	2	46	theme	Thirty-five	403:413	arg1	metabolites					425:435	Thirty-five secondary metabolites	403:435	Thirty-five secondary metabolites	403:435	Thirty-five secondary metabolites were characterized in both extracts using HPLC-PDA-ESI-MS/MS. Phenolic acids, mainly rosmarinic acid and its derivatives, dominated the T. algeriensis extract, while the phenolic diterpene carnosol and the methylated flavonoid salvigenin, prevailed in T. fontanesii extract.
32294957	2	47	theme	HPLC-PDA-ESI-MS/MS.	479:497	arg1	acids					508:512	HPLC-PDA-ESI-MS/MS. Phenolic acids	479:512	HPLC-PDA-ESI-MS/MS. Phenolic acids	479:512	Thirty-five secondary metabolites were characterized in both extracts using HPLC-PDA-ESI-MS/MS. Phenolic acids, mainly rosmarinic acid and its derivatives, dominated the T. algeriensis extract, while the phenolic diterpene carnosol and the methylated flavonoid salvigenin, prevailed in T. fontanesii extract.
32294957	2	47	theme	HPLC-PDA-ESI-MS/MS.	479:497	arg1	acid					533:536	rosmarinic acid	522:536	rosmarinic acid	522:536	Thirty-five secondary metabolites were characterized in both extracts using HPLC-PDA-ESI-MS/MS. Phenolic acids, mainly rosmarinic acid and its derivatives, dominated the T. algeriensis extract, while the phenolic diterpene carnosol and the methylated flavonoid salvigenin, prevailed in T. fontanesii extract.
32294957	2	47	theme	HPLC-PDA-ESI-MS/MS.	479:497	arg1	derivatives					546:556	its derivatives	542:556	its derivatives	542:556	Thirty-five secondary metabolites were characterized in both extracts using HPLC-PDA-ESI-MS/MS. Phenolic acids, mainly rosmarinic acid and its derivatives, dominated the T. algeriensis extract, while the phenolic diterpene carnosol and the methylated flavonoid salvigenin, prevailed in T. fontanesii extract.
32294957	8	48	theme	interesting	1517:1527	arg1	candidates					1529:1538	interesting candidates	1517:1538	interesting candidates for the treatment of inflammation and oxidative stress-related disorders	1517:1611	In conclusion, Thymus algeriensis and Thymus fontanesii may be interesting candidates for the treatment of inflammation and oxidative stress-related disorders.
32294957	3	49	theme	binding	804:810	arg1	affinities					812:821	the binding affinities	800:821	the binding affinities of some individual secondary metabolites from both extracts to the main enzymes involved in the inflammation pathway	800:938	Molecular docking study was carried out to estimate the anti-inflammatory potential and the binding affinities of some individual secondary metabolites from both extracts to the main enzymes involved in the inflammation pathway.
32294957	4	50	dep	in	979:980	arg1	vivo					982:985	vivo	982:985	vivo	982:985	In vitro enzyme inhibitory assays and in vivo assays were used to investigate the antioxidant and anti-inflammatory activities of the extracts.
32294957	4	51	theme	In	941:942	arg1	assays					968:973	In vitro enzyme inhibitory assays	941:973	In vitro enzyme inhibitory assays	941:973	In vitro enzyme inhibitory assays and in vivo assays were used to investigate the antioxidant and anti-inflammatory activities of the extracts.
32294957	5	52	theme	antioxidant	1145:1155	arg1	effects					1204:1210	antioxidant, anti-inflammatory, analgesic, and antipyretic effects	1145:1210	antioxidant, anti-inflammatory, analgesic, and antipyretic effects	1145:1210	Results revealed that both studied Thymus species exhibited antioxidant, anti-inflammatory, analgesic, and antipyretic effects.
32294957	5	53	theme	antipyretic	1192:1202	arg1	effects					1204:1210	antioxidant, anti-inflammatory, analgesic, and antipyretic effects	1145:1210	antioxidant, anti-inflammatory, analgesic, and antipyretic effects	1145:1210	Results revealed that both studied Thymus species exhibited antioxidant, anti-inflammatory, analgesic, and antipyretic effects.
32294957	0	54	theme	Chemical	42:49	arg1	Composition					51:61	Chemical Composition	42:61	Chemical Composition	42:61	Thymus algeriensis and Thymus fontanesii: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison.
32294957	1	55	theme	methanol	312:319	arg1	extracts					321:328	methanol extracts	312:328	methanol extracts from the leaves of Thymus algeriensis and Thymus fontanesii (Lamiaceae)	312:400	This study aimed to investigate the chemical composition, and evaluate the antioxidant, anti-inflammatory, anti-pyretic, and the analgesic properties of methanol extracts from the leaves of Thymus algeriensis and Thymus fontanesii (Lamiaceae).
32294957	0	56	theme	Comprehensive	133:145	arg1	Comparison					147:156	A Comprehensive Comparison	131:156	Thymus algeriensis and Thymus fontanesii: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison.	0:157	Thymus algeriensis and Thymus fontanesii: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison.
32294957	2	57	theme	phenolic	607:614	arg1	carnosol					626:633	the phenolic diterpene carnosol	603:633	the phenolic diterpene carnosol	603:633	Thirty-five secondary metabolites were characterized in both extracts using HPLC-PDA-ESI-MS/MS. Phenolic acids, mainly rosmarinic acid and its derivatives, dominated the T. algeriensis extract, while the phenolic diterpene carnosol and the methylated flavonoid salvigenin, prevailed in T. fontanesii extract.
32294957	1	58	theme	extracts	321:328	arg1	properties					298:307	the analgesic properties	284:307	the analgesic properties of methanol extracts from the leaves of Thymus algeriensis and Thymus fontanesii (Lamiaceae)	284:400	This study aimed to investigate the chemical composition, and evaluate the antioxidant, anti-inflammatory, anti-pyretic, and the analgesic properties of methanol extracts from the leaves of Thymus algeriensis and Thymus fontanesii (Lamiaceae).
32294957	4	59	theme	enzyme	950:955	arg1	assays					968:973	In vitro enzyme inhibitory assays	941:973	In vitro enzyme inhibitory assays	941:973	In vitro enzyme inhibitory assays and in vivo assays were used to investigate the antioxidant and anti-inflammatory activities of the extracts.
32294957	4	60	used	used	999:1002	arg2	assays					968:973	In vitro enzyme inhibitory assays	941:973	In vitro enzyme inhibitory assays	941:973	In vitro enzyme inhibitory assays and in vivo assays were used to investigate the antioxidant and anti-inflammatory activities of the extracts.
32294957	4	60	used	used	999:1002	arg2	assays					987:992	in vivo assays	979:992	in vivo assays	979:992	In vitro enzyme inhibitory assays and in vivo assays were used to investigate the antioxidant and anti-inflammatory activities of the extracts.
32294957	3	61	theme	secondary	842:850	arg1	metabolites					852:862	some individual secondary metabolites	826:862	some individual secondary metabolites from both extracts	826:881	Molecular docking study was carried out to estimate the anti-inflammatory potential and the binding affinities of some individual secondary metabolites from both extracts to the main enzymes involved in the inflammation pathway.
32294957	2	62	theme	algeriensis	576:586	arg1	extract					588:594	the T. algeriensis extract	569:594	the T. algeriensis extract	569:594	Thirty-five secondary metabolites were characterized in both extracts using HPLC-PDA-ESI-MS/MS. Phenolic acids, mainly rosmarinic acid and its derivatives, dominated the T. algeriensis extract, while the phenolic diterpene carnosol and the methylated flavonoid salvigenin, prevailed in T. fontanesii extract.
32294957	6	63	theme	ascorbic	1262:1269	arg1	acid					1271:1274	ascorbic acid	1262:1274	ascorbic acid	1262:1274	They showed to be a more potent antioxidant than ascorbic acid and more selective against cyclooxygenase (COX-2) than diclofenac and indomethacin.
32294957	3	64	theme	metabolites	852:862	arg1	potential					786:794	the anti-inflammatory potential	764:794	the anti-inflammatory potential	764:794	Molecular docking study was carried out to estimate the anti-inflammatory potential and the binding affinities of some individual secondary metabolites from both extracts to the main enzymes involved in the inflammation pathway.
32294957	3	64	theme	metabolites	852:862	arg1	affinities					812:821	the binding affinities	800:821	the binding affinities of some individual secondary metabolites from both extracts to the main enzymes involved in the inflammation pathway	800:938	Molecular docking study was carried out to estimate the anti-inflammatory potential and the binding affinities of some individual secondary metabolites from both extracts to the main enzymes involved in the inflammation pathway.
32294957	0	65	theme	In	64:65	arg1	Composition					51:61	Chemical Composition	42:61	Chemical Composition	42:61	Thymus algeriensis and Thymus fontanesii: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison.
32294957	5	66	theme	studied	1112:1118	arg1	species					1127:1133	both studied Thymus species	1107:1133	both studied Thymus species	1107:1133	Results revealed that both studied Thymus species exhibited antioxidant, anti-inflammatory, analgesic, and antipyretic effects.
32294957	0	67	dep	algeriensis	7:17	arg1	Comparison					147:156	A Comprehensive Comparison	131:156	Thymus algeriensis and Thymus fontanesii: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison.	0:157	Thymus algeriensis and Thymus fontanesii: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison.
32294957	0	67	dep	algeriensis	7:17	arg1	Killing					95:101	Pain Killing	90:101	Pain Killing	90:101	Thymus algeriensis and Thymus fontanesii: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison.
32294957	0	67	dep	algeriensis	7:17	arg1	Activities					119:128	Antipyretic Activities	107:128	Antipyretic Activities	107:128	Thymus algeriensis and Thymus fontanesii: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison.
32294957	0	67	dep	algeriensis	7:17	arg1	Composition					51:61	Chemical Composition	42:61	Chemical Composition	42:61	Thymus algeriensis and Thymus fontanesii: Chemical Composition, In Vivo Antiinflammatory, Pain Killing and Antipyretic Activities: A Comprehensive Comparison.
32294957	3	68	theme	main	890:893	arg1	enzymes					895:901	the main enzymes	886:901	the main enzymes involved in the inflammation pathway	886:938	Molecular docking study was carried out to estimate the anti-inflammatory potential and the binding affinities of some individual secondary metabolites from both extracts to the main enzymes involved in the inflammation pathway.
32294957	8	69	theme	inflammation	1561:1572	arg1	treatment					1548:1556	the treatment	1544:1556	the treatment of inflammation and oxidative stress-related disorders	1544:1611	In conclusion, Thymus algeriensis and Thymus fontanesii may be interesting candidates for the treatment of inflammation and oxidative stress-related disorders.
32294957	3	70	theme	inflammation	919:930	arg1	pathway					932:938	the inflammation pathway	915:938	the inflammation pathway	915:938	Molecular docking study was carried out to estimate the anti-inflammatory potential and the binding affinities of some individual secondary metabolites from both extracts to the main enzymes involved in the inflammation pathway.
32294957	3	71	theme	individual	831:840	arg1	metabolites					852:862	some individual secondary metabolites	826:862	some individual secondary metabolites from both extracts	826:881	Molecular docking study was carried out to estimate the anti-inflammatory potential and the binding affinities of some individual secondary metabolites from both extracts to the main enzymes involved in the inflammation pathway.
32722157	4	0	theme	insulin	610:616	arg1	resistance					618:627	insulin resistance	610:627	insulin resistance	610:627	Maternal glucose homeostasis (fasting plasma glucose and insulin, homeostatic model assessment for insulin resistance, and insulin sensitivity index) was evaluated at 30 weeks of gestation in healthy women (body mass index = 18.5-35 kg/m2).
32722157	9	1	theme	trimester	1198:1206	arg1	glucose					1223:1229	third trimester fasting plasma glucose	1192:1229	third trimester fasting plasma glucose	1192:1229	In non-secretors, third trimester fasting plasma glucose and insulin were negatively associated with total HMO-bound sialic acid and concentrations of the sialylated HMOs 3'-sialyllactose and disialylacto-N-tetraose.
32722157	1	2	theme	critical	257:264	arg1	role					266:269	a critical role	255:269	a critical role	255:269	Human milk oligosaccharides (HMOs) are bioactive molecules in human milk that play a critical role in infant health.
32722157	9	3	theme	plasma	1216:1221	arg1	glucose					1223:1229	third trimester fasting plasma glucose	1192:1229	third trimester fasting plasma glucose	1192:1229	In non-secretors, third trimester fasting plasma glucose and insulin were negatively associated with total HMO-bound sialic acid and concentrations of the sialylated HMOs 3'-sialyllactose and disialylacto-N-tetraose.
32722157	11	4	theme	secretor	1703:1710	arg1	populations					1729:1739	both secretor and non-secretor populations	1698:1739	populations	1729:1739	This study is the first to demonstrate a relationship between obesity-associated maternal factors and HMO composition in both secretor and non-secretor populations.
32722157	4	5	theme	gestation	690:698	arg1	weeks					681:685	30 weeks	678:685	30 weeks of gestation	678:698	Maternal glucose homeostasis (fasting plasma glucose and insulin, homeostatic model assessment for insulin resistance, and insulin sensitivity index) was evaluated at 30 weeks of gestation in healthy women (body mass index = 18.5-35 kg/m2).
32722157	2	6	theme	metabolic	312:320	arg1	aberrations					322:332	associated metabolic aberrations	301:332	associated metabolic aberrations	301:332	Obesity and associated metabolic aberrations can negatively impact lactation and alter milk composition.
32722157	11	7	theme	HMO	1679:1681	arg1	composition					1683:1693	HMO composition	1679:1693	HMO composition	1679:1693	This study is the first to demonstrate a relationship between obesity-associated maternal factors and HMO composition in both secretor and non-secretor populations.
32722157	0	8	theme	Months	131:136	arg1	Postpartum					138:147	2 Months Postpartum	129:147	2 Months Postpartum	129:147	Third-Trimester Glucose Homeostasis in Healthy Women Is Differentially Associated with Human Milk Oligosaccharide Composition at 2 Months Postpartum by Secretor Phenotype.
32722157	1	9	theme	bioactive	211:219	arg1	oligosaccharides					183:198	Human milk oligosaccharides	172:198	Human milk oligosaccharides (HMOs)	172:205	Human milk oligosaccharides (HMOs) are bioactive molecules in human milk that play a critical role in infant health.
32722157	1	9	theme	bioactive	211:219	arg1	molecules					221:229	bioactive molecules	211:229	bioactive molecules in human milk that play a critical role in infant health	211:286	Human milk oligosaccharides (HMOs) are bioactive molecules in human milk that play a critical role in infant health.
32722157	9	10	theme	total	1275:1279	arg1	acid					1298:1301	total HMO-bound sialic acid	1275:1301	total HMO-bound sialic acid	1275:1301	In non-secretors, third trimester fasting plasma glucose and insulin were negatively associated with total HMO-bound sialic acid and concentrations of the sialylated HMOs 3'-sialyllactose and disialylacto-N-tetraose.
32722157	1	11	from	molecules	221:229	arg1	milk					240:243	human milk	234:243	human milk	234:243	Human milk oligosaccharides (HMOs) are bioactive molecules in human milk that play a critical role in infant health.
32722157	4	12	theme	mass	723:726	arg1	index					728:732	body mass index	718:732	body mass index = 18.5-35 kg/m2	718:748	Maternal glucose homeostasis (fasting plasma glucose and insulin, homeostatic model assessment for insulin resistance, and insulin sensitivity index) was evaluated at 30 weeks of gestation in healthy women (body mass index = 18.5-35 kg/m2).
32722157	9	13	theme	sialic	1291:1296	arg1	acid					1298:1301	total HMO-bound sialic acid	1275:1301	total HMO-bound sialic acid	1275:1301	In non-secretors, third trimester fasting plasma glucose and insulin were negatively associated with total HMO-bound sialic acid and concentrations of the sialylated HMOs 3'-sialyllactose and disialylacto-N-tetraose.
32722157	6	14	theme	HMO	812:814	arg1	concentrations					816:829	HMO concentrations	812:829	HMO concentrations	812:829	HMO concentrations were measured via high performance liquid chromatography.
32722157	4	15	theme	=	734:734	arg1	index					728:732	body mass index	718:732	body mass index = 18.5-35 kg/m2	718:748	Maternal glucose homeostasis (fasting plasma glucose and insulin, homeostatic model assessment for insulin resistance, and insulin sensitivity index) was evaluated at 30 weeks of gestation in healthy women (body mass index = 18.5-35 kg/m2).
32722157	4	16	theme	healthy	703:709	arg1	women					711:715	healthy women	703:715	healthy women (body mass index = 18.5-35 kg/m2)	703:749	Maternal glucose homeostasis (fasting plasma glucose and insulin, homeostatic model assessment for insulin resistance, and insulin sensitivity index) was evaluated at 30 weeks of gestation in healthy women (body mass index = 18.5-35 kg/m2).
32722157	7	17	dep	concentrations	981:994	arg1	nmol/mL					1002:1008	<100 nmol/mL	997:1008	<100 nmol/mL	997:1008	Women were categorized into "secretor" and "non-secretor" groups based on 2'-Fucosyllactose concentrations (<100 nmol/mL, non-secretor).
32722157	7	17	dep	concentrations	981:994	arg1	non-secretor					1011:1022	non-secretor	1011:1022	non-secretor	1011:1022	Women were categorized into "secretor" and "non-secretor" groups based on 2'-Fucosyllactose concentrations (<100 nmol/mL, non-secretor).
32722157	5	18	theme	milk	758:761	arg1	samples					763:769	Human milk samples	752:769	Human milk samples	752:769	Human milk samples were collected at two months postpartum.
32722157	0	19	from	Homeostasis	24:34	arg1	Women					47:51	Healthy Women	39:51	Healthy Women	39:51	Third-Trimester Glucose Homeostasis in Healthy Women Is Differentially Associated with Human Milk Oligosaccharide Composition at 2 Months Postpartum by Secretor Phenotype.
32722157	11	20	theme	maternal	1658:1665	arg1	factors					1667:1673	obesity-associated maternal factors	1639:1673	obesity-associated maternal factors	1639:1673	This study is the first to demonstrate a relationship between obesity-associated maternal factors and HMO composition in both secretor and non-secretor populations.
32722157	0	21	theme	Human	87:91	arg1	Composition					114:124	Human Milk Oligosaccharide Composition	87:124	Human Milk Oligosaccharide Composition at 2 Months Postpartum by Secretor Phenotype	87:169	Third-Trimester Glucose Homeostasis in Healthy Women Is Differentially Associated with Human Milk Oligosaccharide Composition at 2 Months Postpartum by Secretor Phenotype.
32722157	9	22	theme	sialylated	1329:1338	arg1	3'-sialyllactose					1345:1360	the sialylated HMOs 3'-sialyllactose	1325:1360	the sialylated HMOs 3'-sialyllactose	1325:1360	In non-secretors, third trimester fasting plasma glucose and insulin were negatively associated with total HMO-bound sialic acid and concentrations of the sialylated HMOs 3'-sialyllactose and disialylacto-N-tetraose.
32722157	7	23	theme	2'-Fucosyllactose	963:979	arg1	concentrations					981:994	2'-Fucosyllactose concentrations	963:994	2'-Fucosyllactose concentrations (<100 nmol/mL, non-secretor)	963:1023	Women were categorized into "secretor" and "non-secretor" groups based on 2'-Fucosyllactose concentrations (<100 nmol/mL, non-secretor).
32722157	1	24	theme	milk	178:181	arg1	HMOs					201:204	HMOs	201:204	HMOs	201:204	Human milk oligosaccharides (HMOs) are bioactive molecules in human milk that play a critical role in infant health.
32722157	1	24	theme	milk	178:181	arg1	oligosaccharides					183:198	Human milk oligosaccharides	172:198	Human milk oligosaccharides (HMOs)	172:205	Human milk oligosaccharides (HMOs) are bioactive molecules in human milk that play a critical role in infant health.
32722157	1	24	theme	milk	178:181	arg1	molecules					221:229	bioactive molecules	211:229	bioactive molecules in human milk that play a critical role in infant health	211:286	Human milk oligosaccharides (HMOs) are bioactive molecules in human milk that play a critical role in infant health.
32722157	10	25	theme	sialyllacto-N-tetraose	1479:1500	arg1	c					1502:1502	sialyllacto-N-tetraose c	1479:1502	sialyllacto-N-tetraose c	1479:1502	In secretors, difucosyllactose and lacto-N-fucopentaose-II concentrations increased and sialyllacto-N-tetraose c and sialyllacto-N-tetraose b decreased as insulin sensitivity increased.
32722157	0	26	theme	Oligosaccharide	98:112	arg1	Composition					114:124	Human Milk Oligosaccharide Composition	87:124	Human Milk Oligosaccharide Composition at 2 Months Postpartum by Secretor Phenotype	87:169	Third-Trimester Glucose Homeostasis in Healthy Women Is Differentially Associated with Human Milk Oligosaccharide Composition at 2 Months Postpartum by Secretor Phenotype.
32722157	9	27	theme	3'-sialyllactose	1345:1360	arg1	acid					1298:1301	total HMO-bound sialic acid	1275:1301	total HMO-bound sialic acid	1275:1301	In non-secretors, third trimester fasting plasma glucose and insulin were negatively associated with total HMO-bound sialic acid and concentrations of the sialylated HMOs 3'-sialyllactose and disialylacto-N-tetraose.
32722157	9	27	theme	3'-sialyllactose	1345:1360	arg1	concentrations					1307:1320	concentrations	1307:1320	concentrations of the sialylated HMOs 3'-sialyllactose and disialylacto-N-tetraose	1307:1388	In non-secretors, third trimester fasting plasma glucose and insulin were negatively associated with total HMO-bound sialic acid and concentrations of the sialylated HMOs 3'-sialyllactose and disialylacto-N-tetraose.
32722157	4	28	dep	women	711:715	arg1	index					728:732	body mass index	718:732	body mass index = 18.5-35 kg/m2	718:748	Maternal glucose homeostasis (fasting plasma glucose and insulin, homeostatic model assessment for insulin resistance, and insulin sensitivity index) was evaluated at 30 weeks of gestation in healthy women (body mass index = 18.5-35 kg/m2).
32722157	3	29	theme	HMO	458:460	arg1	composition					462:472	HMO composition	458:472	HMO composition	458:472	Here, the relationship between maternal glucose homeostasis and HMO composition from 136 healthy women was examined.
32722157	9	30	theme	disialylacto-N-tetraose	1366:1388	arg1	acid					1298:1301	total HMO-bound sialic acid	1275:1301	total HMO-bound sialic acid	1275:1301	In non-secretors, third trimester fasting plasma glucose and insulin were negatively associated with total HMO-bound sialic acid and concentrations of the sialylated HMOs 3'-sialyllactose and disialylacto-N-tetraose.
32722157	9	30	theme	disialylacto-N-tetraose	1366:1388	arg1	concentrations					1307:1320	concentrations	1307:1320	concentrations of the sialylated HMOs 3'-sialyllactose and disialylacto-N-tetraose	1307:1388	In non-secretors, third trimester fasting plasma glucose and insulin were negatively associated with total HMO-bound sialic acid and concentrations of the sialylated HMOs 3'-sialyllactose and disialylacto-N-tetraose.
32722157	8	31	theme	linear	1061:1066	arg1	models					1068:1073	linear models	1061:1073	linear models	1061:1073	Pearson's correlation analysis and linear models were used to assess the relationships between maternal glucose homeostasis and HMO concentrations.
32722157	7	32	theme	"	945:945	arg1	groups					947:952	"secretor" and "non-secretor" groups	917:952	"secretor" and "non-secretor" groups based on 2'-Fucosyllactose concentrations (<100 nmol/mL, non-secretor)	917:1023	Women were categorized into "secretor" and "non-secretor" groups based on 2'-Fucosyllactose concentrations (<100 nmol/mL, non-secretor).
32722157	0	33	theme	Third-Trimester	0:14	arg1	Homeostasis					24:34	Third-Trimester Glucose Homeostasis	0:34	Third-Trimester Glucose Homeostasis in Healthy Women	0:51	Third-Trimester Glucose Homeostasis in Healthy Women Is Differentially Associated with Human Milk Oligosaccharide Composition at 2 Months Postpartum by Secretor Phenotype.
32722157	1	34	theme	infant	274:279	arg1	health					281:286	infant health	274:286	infant health	274:286	Human milk oligosaccharides (HMOs) are bioactive molecules in human milk that play a critical role in infant health.
32722157	3	35	theme	healthy	483:489	arg1	women					491:495	136 healthy women	479:495	136 healthy women	479:495	Here, the relationship between maternal glucose homeostasis and HMO composition from 136 healthy women was examined.
32722157	3	36	theme	maternal	425:432	arg1	homeostasis					442:452	maternal glucose homeostasis	425:452	maternal glucose homeostasis	425:452	Here, the relationship between maternal glucose homeostasis and HMO composition from 136 healthy women was examined.
32722157	4	37	theme	model	589:593	arg1	assessment					595:604	homeostatic model assessment	577:604	homeostatic model assessment for insulin resistance	577:627	Maternal glucose homeostasis (fasting plasma glucose and insulin, homeostatic model assessment for insulin resistance, and insulin sensitivity index) was evaluated at 30 weeks of gestation in healthy women (body mass index = 18.5-35 kg/m2).
32722157	0	38	theme	Healthy	39:45	arg1	Women					47:51	Healthy Women	39:51	Healthy Women	39:51	Third-Trimester Glucose Homeostasis in Healthy Women Is Differentially Associated with Human Milk Oligosaccharide Composition at 2 Months Postpartum by Secretor Phenotype.
32722157	6	39	theme	liquid	866:871	arg1	chromatography					873:886	high performance liquid chromatography	849:886	high performance liquid chromatography	849:886	HMO concentrations were measured via high performance liquid chromatography.
32722157	4	40	theme	plasma	549:554	arg1	glucose					556:562	fasting plasma glucose	541:562	fasting plasma glucose	541:562	Maternal glucose homeostasis (fasting plasma glucose and insulin, homeostatic model assessment for insulin resistance, and insulin sensitivity index) was evaluated at 30 weeks of gestation in healthy women (body mass index = 18.5-35 kg/m2).
32722157	5	41	theme	months	793:798	arg1	postpartum					800:809	two months postpartum	789:809	two months postpartum	789:809	Human milk samples were collected at two months postpartum.
32722157	4	42	dep	homeostasis	528:538	arg1	insulin					568:574	insulin	568:574	insulin	568:574	Maternal glucose homeostasis (fasting plasma glucose and insulin, homeostatic model assessment for insulin resistance, and insulin sensitivity index) was evaluated at 30 weeks of gestation in healthy women (body mass index = 18.5-35 kg/m2).
32722157	4	42	dep	homeostasis	528:538	arg1	glucose					556:562	fasting plasma glucose	541:562	fasting plasma glucose	541:562	Maternal glucose homeostasis (fasting plasma glucose and insulin, homeostatic model assessment for insulin resistance, and insulin sensitivity index) was evaluated at 30 weeks of gestation in healthy women (body mass index = 18.5-35 kg/m2).
32722157	4	42	dep	homeostasis	528:538	arg1	assessment					595:604	homeostatic model assessment	577:604	homeostatic model assessment for insulin resistance	577:627	Maternal glucose homeostasis (fasting plasma glucose and insulin, homeostatic model assessment for insulin resistance, and insulin sensitivity index) was evaluated at 30 weeks of gestation in healthy women (body mass index = 18.5-35 kg/m2).
32722157	4	42	dep	homeostasis	528:538	arg1	index					654:658	insulin sensitivity index	634:658	insulin sensitivity index	634:658	Maternal glucose homeostasis (fasting plasma glucose and insulin, homeostatic model assessment for insulin resistance, and insulin sensitivity index) was evaluated at 30 weeks of gestation in healthy women (body mass index = 18.5-35 kg/m2).
32722157	6	43	theme	high	849:852	arg1	chromatography					873:886	high performance liquid chromatography	849:886	high performance liquid chromatography	849:886	HMO concentrations were measured via high performance liquid chromatography.
32722157	3	44	from	women	491:495	arg1	homeostasis					442:452	maternal glucose homeostasis	425:452	maternal glucose homeostasis	425:452	Here, the relationship between maternal glucose homeostasis and HMO composition from 136 healthy women was examined.
32722157	3	44	from	women	491:495	arg1	composition					462:472	HMO composition	458:472	HMO composition	458:472	Here, the relationship between maternal glucose homeostasis and HMO composition from 136 healthy women was examined.
32722157	1	45	theme	human	234:238	arg1	milk					240:243	human milk	234:243	human milk	234:243	Human milk oligosaccharides (HMOs) are bioactive molecules in human milk that play a critical role in infant health.
32722157	8	46	theme	glucose	1130:1136	arg1	homeostasis					1138:1148	maternal glucose homeostasis	1121:1148	maternal glucose homeostasis	1121:1148	Pearson's correlation analysis and linear models were used to assess the relationships between maternal glucose homeostasis and HMO concentrations.
32722157	7	47	theme	secretor	918:925	arg1	"					926:926	"secretor"	917:926	"secretor"	917:926	Women were categorized into "secretor" and "non-secretor" groups based on 2'-Fucosyllactose concentrations (<100 nmol/mL, non-secretor).
32722157	4	48	theme	sensitivity	642:652	arg1	index					654:658	insulin sensitivity index	634:658	insulin sensitivity index	634:658	Maternal glucose homeostasis (fasting plasma glucose and insulin, homeostatic model assessment for insulin resistance, and insulin sensitivity index) was evaluated at 30 weeks of gestation in healthy women (body mass index = 18.5-35 kg/m2).
32722157	9	49	theme	third	1192:1196	arg1	glucose					1223:1229	third trimester fasting plasma glucose	1192:1229	third trimester fasting plasma glucose	1192:1229	In non-secretors, third trimester fasting plasma glucose and insulin were negatively associated with total HMO-bound sialic acid and concentrations of the sialylated HMOs 3'-sialyllactose and disialylacto-N-tetraose.
32722157	4	50	theme	glucose	520:526	arg1	homeostasis					528:538	Maternal glucose homeostasis	511:538	Maternal glucose homeostasis (fasting plasma glucose and insulin, homeostatic model assessment for insulin resistance, and insulin sensitivity index)	511:659	Maternal glucose homeostasis (fasting plasma glucose and insulin, homeostatic model assessment for insulin resistance, and insulin sensitivity index) was evaluated at 30 weeks of gestation in healthy women (body mass index = 18.5-35 kg/m2).
32722157	9	51	theme	fasting	1208:1214	arg1	glucose					1223:1229	third trimester fasting plasma glucose	1192:1229	third trimester fasting plasma glucose	1192:1229	In non-secretors, third trimester fasting plasma glucose and insulin were negatively associated with total HMO-bound sialic acid and concentrations of the sialylated HMOs 3'-sialyllactose and disialylacto-N-tetraose.
32722157	10	52	theme	sialyllacto-N-tetraose	1508:1529	arg1	b					1531:1531	sialyllacto-N-tetraose b	1508:1531	sialyllacto-N-tetraose b	1508:1531	In secretors, difucosyllactose and lacto-N-fucopentaose-II concentrations increased and sialyllacto-N-tetraose c and sialyllacto-N-tetraose b decreased as insulin sensitivity increased.
32722157	2	53	theme	associated	301:310	arg1	aberrations					322:332	associated metabolic aberrations	301:332	associated metabolic aberrations	301:332	Obesity and associated metabolic aberrations can negatively impact lactation and alter milk composition.
32722157	11	54	from	composition	1683:1693	arg1	populations					1729:1739	both secretor and non-secretor populations	1698:1739	populations	1729:1739	This study is the first to demonstrate a relationship between obesity-associated maternal factors and HMO composition in both secretor and non-secretor populations.
32722157	0	55	theme	Secretor	152:159	arg1	Phenotype					161:169	Secretor Phenotype	152:169	Secretor Phenotype	152:169	Third-Trimester Glucose Homeostasis in Healthy Women Is Differentially Associated with Human Milk Oligosaccharide Composition at 2 Months Postpartum by Secretor Phenotype.
32722157	9	56	theme	HMO-bound	1281:1289	arg1	acid					1298:1301	total HMO-bound sialic acid	1275:1301	total HMO-bound sialic acid	1275:1301	In non-secretors, third trimester fasting plasma glucose and insulin were negatively associated with total HMO-bound sialic acid and concentrations of the sialylated HMOs 3'-sialyllactose and disialylacto-N-tetraose.
32722157	4	57	theme	body	718:721	arg1	index					728:732	body mass index	718:732	body mass index = 18.5-35 kg/m2	718:748	Maternal glucose homeostasis (fasting plasma glucose and insulin, homeostatic model assessment for insulin resistance, and insulin sensitivity index) was evaluated at 30 weeks of gestation in healthy women (body mass index = 18.5-35 kg/m2).
32722157	9	58	gly	sialylated	1329:1338	arg1	3'-sialyllactose					1345:1360	the sialylated HMOs 3'-sialyllactose	1325:1360	the sialylated HMOs 3'-sialyllactose	1325:1360	In non-secretors, third trimester fasting plasma glucose and insulin were negatively associated with total HMO-bound sialic acid and concentrations of the sialylated HMOs 3'-sialyllactose and disialylacto-N-tetraose.
32722157	5	59	theme	Human	752:756	arg1	samples					763:769	Human milk samples	752:769	Human milk samples	752:769	Human milk samples were collected at two months postpartum.
32722157	11	60	from	factors	1667:1673	arg1	populations					1729:1739	both secretor and non-secretor populations	1698:1739	populations	1729:1739	This study is the first to demonstrate a relationship between obesity-associated maternal factors and HMO composition in both secretor and non-secretor populations.
32722157	11	61	theme	obesity-associated	1639:1656	arg1	factors					1667:1673	obesity-associated maternal factors	1639:1673	obesity-associated maternal factors	1639:1673	This study is the first to demonstrate a relationship between obesity-associated maternal factors and HMO composition in both secretor and non-secretor populations.
32722157	0	62	theme	Milk	93:96	arg1	Composition					114:124	Human Milk Oligosaccharide Composition	87:124	Human Milk Oligosaccharide Composition at 2 Months Postpartum by Secretor Phenotype	87:169	Third-Trimester Glucose Homeostasis in Healthy Women Is Differentially Associated with Human Milk Oligosaccharide Composition at 2 Months Postpartum by Secretor Phenotype.
32722157	8	63	theme	correlation	1036:1046	arg1	analysis					1048:1055	Pearson's correlation analysis	1026:1055	Pearson's correlation analysis	1026:1055	Pearson's correlation analysis and linear models were used to assess the relationships between maternal glucose homeostasis and HMO concentrations.
32722157	1	64	theme	Human	172:176	arg1	HMOs					201:204	HMOs	201:204	HMOs	201:204	Human milk oligosaccharides (HMOs) are bioactive molecules in human milk that play a critical role in infant health.
32722157	1	64	theme	Human	172:176	arg1	oligosaccharides					183:198	Human milk oligosaccharides	172:198	Human milk oligosaccharides (HMOs)	172:205	Human milk oligosaccharides (HMOs) are bioactive molecules in human milk that play a critical role in infant health.
32722157	1	64	theme	Human	172:176	arg1	molecules					221:229	bioactive molecules	211:229	bioactive molecules in human milk that play a critical role in infant health	211:286	Human milk oligosaccharides (HMOs) are bioactive molecules in human milk that play a critical role in infant health.
32722157	4	65	theme	fasting	541:547	arg1	glucose					556:562	fasting plasma glucose	541:562	fasting plasma glucose	541:562	Maternal glucose homeostasis (fasting plasma glucose and insulin, homeostatic model assessment for insulin resistance, and insulin sensitivity index) was evaluated at 30 weeks of gestation in healthy women (body mass index = 18.5-35 kg/m2).
32722157	9	66	theme	HMOs	1340:1343	arg1	3'-sialyllactose					1345:1360	the sialylated HMOs 3'-sialyllactose	1325:1360	the sialylated HMOs 3'-sialyllactose	1325:1360	In non-secretors, third trimester fasting plasma glucose and insulin were negatively associated with total HMO-bound sialic acid and concentrations of the sialylated HMOs 3'-sialyllactose and disialylacto-N-tetraose.
32722157	8	67	used	used	1080:1083	arg2	models					1068:1073	linear models	1061:1073	linear models	1061:1073	Pearson's correlation analysis and linear models were used to assess the relationships between maternal glucose homeostasis and HMO concentrations.
32722157	8	67	used	used	1080:1083	arg2	analysis					1048:1055	Pearson's correlation analysis	1026:1055	Pearson's correlation analysis	1026:1055	Pearson's correlation analysis and linear models were used to assess the relationships between maternal glucose homeostasis and HMO concentrations.
32722157	10	68	theme	insulin	1546:1552	arg1	sensitivity					1554:1564	insulin sensitivity	1546:1564	insulin sensitivity	1546:1564	In secretors, difucosyllactose and lacto-N-fucopentaose-II concentrations increased and sialyllacto-N-tetraose c and sialyllacto-N-tetraose b decreased as insulin sensitivity increased.
32722157	4	69	theme	Maternal	511:518	arg1	homeostasis					528:538	Maternal glucose homeostasis	511:538	Maternal glucose homeostasis (fasting plasma glucose and insulin, homeostatic model assessment for insulin resistance, and insulin sensitivity index)	511:659	Maternal glucose homeostasis (fasting plasma glucose and insulin, homeostatic model assessment for insulin resistance, and insulin sensitivity index) was evaluated at 30 weeks of gestation in healthy women (body mass index = 18.5-35 kg/m2).
32722157	0	70	theme	Glucose	16:22	arg1	Homeostasis					24:34	Third-Trimester Glucose Homeostasis	0:34	Third-Trimester Glucose Homeostasis in Healthy Women	0:51	Third-Trimester Glucose Homeostasis in Healthy Women Is Differentially Associated with Human Milk Oligosaccharide Composition at 2 Months Postpartum by Secretor Phenotype.
32722157	4	71	dep	=	734:734	arg1	kg/m2					744:748	18.5-35 kg/m2	736:748	18.5-35 kg/m2	736:748	Maternal glucose homeostasis (fasting plasma glucose and insulin, homeostatic model assessment for insulin resistance, and insulin sensitivity index) was evaluated at 30 weeks of gestation in healthy women (body mass index = 18.5-35 kg/m2).
32722157	4	72	theme	homeostatic	577:587	arg1	assessment					595:604	homeostatic model assessment	577:604	homeostatic model assessment for insulin resistance	577:627	Maternal glucose homeostasis (fasting plasma glucose and insulin, homeostatic model assessment for insulin resistance, and insulin sensitivity index) was evaluated at 30 weeks of gestation in healthy women (body mass index = 18.5-35 kg/m2).
32722157	7	73	theme	non-secretor	933:944	arg1	"					945:945	"non-secretor"	932:945	"non-secretor"	932:945	Women were categorized into "secretor" and "non-secretor" groups based on 2'-Fucosyllactose concentrations (<100 nmol/mL, non-secretor).
32722157	3	74	theme	glucose	434:440	arg1	homeostasis					442:452	maternal glucose homeostasis	425:452	maternal glucose homeostasis	425:452	Here, the relationship between maternal glucose homeostasis and HMO composition from 136 healthy women was examined.
32722157	10	75	theme	difucosyllactose	1405:1420	arg1	concentrations					1450:1463	difucosyllactose and lacto-N-fucopentaose-II concentrations	1405:1463	difucosyllactose and lacto-N-fucopentaose-II concentrations	1405:1463	In secretors, difucosyllactose and lacto-N-fucopentaose-II concentrations increased and sialyllacto-N-tetraose c and sialyllacto-N-tetraose b decreased as insulin sensitivity increased.
32722157	3	76	theme	136	479:481	arg1	women					491:495	136 healthy women	479:495	136 healthy women	479:495	Here, the relationship between maternal glucose homeostasis and HMO composition from 136 healthy women was examined.
32722157	8	77	theme	maternal	1121:1128	arg1	homeostasis					1138:1148	maternal glucose homeostasis	1121:1148	maternal glucose homeostasis	1121:1148	Pearson's correlation analysis and linear models were used to assess the relationships between maternal glucose homeostasis and HMO concentrations.
32722157	6	78	theme	performance	854:864	arg1	chromatography					873:886	high performance liquid chromatography	849:886	high performance liquid chromatography	849:886	HMO concentrations were measured via high performance liquid chromatography.
32722157	11	79	theme	non-secretor	1716:1727	arg1	populations					1729:1739	both secretor and non-secretor populations	1698:1739	populations	1729:1739	This study is the first to demonstrate a relationship between obesity-associated maternal factors and HMO composition in both secretor and non-secretor populations.
32722157	7	80	theme	"	926:926	arg1	groups					947:952	"secretor" and "non-secretor" groups	917:952	"secretor" and "non-secretor" groups based on 2'-Fucosyllactose concentrations (<100 nmol/mL, non-secretor)	917:1023	Women were categorized into "secretor" and "non-secretor" groups based on 2'-Fucosyllactose concentrations (<100 nmol/mL, non-secretor).
32722157	4	81	theme	insulin	634:640	arg1	index					654:658	insulin sensitivity index	634:658	insulin sensitivity index	634:658	Maternal glucose homeostasis (fasting plasma glucose and insulin, homeostatic model assessment for insulin resistance, and insulin sensitivity index) was evaluated at 30 weeks of gestation in healthy women (body mass index = 18.5-35 kg/m2).
32722157	8	82	theme	HMO	1154:1156	arg1	concentrations					1158:1171	HMO concentrations	1154:1171	HMO concentrations	1154:1171	Pearson's correlation analysis and linear models were used to assess the relationships between maternal glucose homeostasis and HMO concentrations.
32722157	0	83	from	Postpartum	138:147	arg1	Composition					114:124	Human Milk Oligosaccharide Composition	87:124	Human Milk Oligosaccharide Composition at 2 Months Postpartum by Secretor Phenotype	87:169	Third-Trimester Glucose Homeostasis in Healthy Women Is Differentially Associated with Human Milk Oligosaccharide Composition at 2 Months Postpartum by Secretor Phenotype.
32722157	10	84	theme	lacto-N-fucopentaose-II	1426:1448	arg1	concentrations					1450:1463	difucosyllactose and lacto-N-fucopentaose-II concentrations	1405:1463	difucosyllactose and lacto-N-fucopentaose-II concentrations	1405:1463	In secretors, difucosyllactose and lacto-N-fucopentaose-II concentrations increased and sialyllacto-N-tetraose c and sialyllacto-N-tetraose b decreased as insulin sensitivity increased.
32722157	2	85	theme	milk	376:379	arg1	composition					381:391	milk composition	376:391	milk composition	376:391	Obesity and associated metabolic aberrations can negatively impact lactation and alter milk composition.
32131144	5	0	theme	expressed	734:742	arg1	DEPs					754:757	DEPs	754:757	DEPs	754:757	52 common differentially expressed proteins (DEPs) are identified, in three ALF groups, compared to the control.
32131144	5	0	theme	expressed	734:742	arg1	proteins					744:751	52 common differentially expressed proteins	709:751	52 common differentially expressed proteins (DEPs)	709:758	52 common differentially expressed proteins (DEPs) are identified, in three ALF groups, compared to the control.
32131144	6	1	theme	metabolic	949:957	arg1	process					959:965	metabolic process	949:965	metabolic process	949:965	Gene ontology analyses show that among the common DEPs, ten proteins are involved in immune system process, and 39 proteins in metabolic process.
32131144	8	2	theme	mouse	1520:1524	arg1	proteins					1532:1539	mouse liver proteins	1520:1539	mouse liver proteins	1520:1539	In conclusion, the common and specific DEPs in three ALF mouse models at molecular level are identified; and determined a close-to-complete reference map of mouse liver proteins which will be useful for clinical diagnosis and treatment of liver failure in humans.
32131144	6	3	from	proteins	937:944	arg1	process					959:965	metabolic process	949:965	metabolic process	949:965	Gene ontology analyses show that among the common DEPs, ten proteins are involved in immune system process, and 39 proteins in metabolic process.
32131144	3	4	theme	molecular	498:506	arg1	unclear					599:605	unclear	599:605	unclear	599:605	However, the molecular differences among three ALF models (LPS/D-GalN, poly(I:C)/D-GalN, and CpG DNA/D-GalN), are unclear.
32131144	3	4	theme	molecular	498:506	arg1	differences					508:518	the molecular differences	494:518	the molecular differences	494:518	However, the molecular differences among three ALF models (LPS/D-GalN, poly(I:C)/D-GalN, and CpG DNA/D-GalN), are unclear.
32131144	8	5	theme	proteins	1532:1539	arg1	map					1513:1515	a close-to-complete reference map	1483:1515	a close-to-complete reference map of mouse liver proteins which will be useful for clinical diagnosis and treatment of liver failure in humans	1483:1624	In conclusion, the common and specific DEPs in three ALF mouse models at molecular level are identified; and determined a close-to-complete reference map of mouse liver proteins which will be useful for clinical diagnosis and treatment of liver failure in humans.
32131144	8	5	theme	proteins	1532:1539	arg1	useful					1555:1560	useful	1555:1560	useful	1555:1560	In conclusion, the common and specific DEPs in three ALF mouse models at molecular level are identified; and determined a close-to-complete reference map of mouse liver proteins which will be useful for clinical diagnosis and treatment of liver failure in humans.
32131144	7	6	theme	DNA/D-GalN-specific	1282:1300	arg1	proteins					1302:1309	the membrane and CpG DNA/D-GalN-specific proteins	1261:1309	proteins	1302:1309	Among 80,195, and 23 specifically-expressed proteins in poly(I:C)/D-GalN, LPS/D-GalN, and CpG DNA/D-GalN groups, LPS/D-GalN-specific proteins are mostly distributed in the endoplasmic reticulum and more enriched in metabolic pathways, whereas poly (I:C)/D-GalN-specific proteins are mainly in the membrane and CpG DNA/D-GalN-specific proteins are related to the ribosome structural composition.
32131144	7	7	theme	/D-GalN-specific	1221:1236	arg1	proteins					1238:1245	poly (I:C)/D-GalN-specific proteins	1211:1245	poly (I:C)/D-GalN-specific proteins	1211:1245	Among 80,195, and 23 specifically-expressed proteins in poly(I:C)/D-GalN, LPS/D-GalN, and CpG DNA/D-GalN groups, LPS/D-GalN-specific proteins are mostly distributed in the endoplasmic reticulum and more enriched in metabolic pathways, whereas poly (I:C)/D-GalN-specific proteins are mainly in the membrane and CpG DNA/D-GalN-specific proteins are related to the ribosome structural composition.
32131144	8	8	from	DEPs	1402:1405	arg1	models					1426:1431	three ALF mouse models	1410:1431	three ALF mouse models	1410:1431	In conclusion, the common and specific DEPs in three ALF mouse models at molecular level are identified; and determined a close-to-complete reference map of mouse liver proteins which will be useful for clinical diagnosis and treatment of liver failure in humans.
32131144	6	9	from	process	921:927	arg1	process					959:965	metabolic process	949:965	metabolic process	949:965	Gene ontology analyses show that among the common DEPs, ten proteins are involved in immune system process, and 39 proteins in metabolic process.
32131144	3	10	theme	ALF	532:534	arg1	/D-GalN					565:571	poly(I:C)/D-GalN	556:571	poly(I:C)/D-GalN	556:571	However, the molecular differences among three ALF models (LPS/D-GalN, poly(I:C)/D-GalN, and CpG DNA/D-GalN), are unclear.
32131144	3	10	theme	ALF	532:534	arg1	LPS/D-GalN					544:553	LPS/D-GalN	544:553	LPS/D-GalN	544:553	However, the molecular differences among three ALF models (LPS/D-GalN, poly(I:C)/D-GalN, and CpG DNA/D-GalN), are unclear.
32131144	3	10	theme	ALF	532:534	arg1	models					536:541	three ALF models	526:541	three ALF models (LPS/D-GalN, poly(I:C)/D-GalN, and CpG DNA/D-GalN)	526:592	However, the molecular differences among three ALF models (LPS/D-GalN, poly(I:C)/D-GalN, and CpG DNA/D-GalN), are unclear.
32131144	3	10	theme	ALF	532:534	arg1	DNA/D-GalN					582:591	CpG DNA/D-GalN	578:591	CpG DNA/D-GalN	578:591	However, the molecular differences among three ALF models (LPS/D-GalN, poly(I:C)/D-GalN, and CpG DNA/D-GalN), are unclear.
32131144	1	11	theme	abrupt	184:189	arg1	injury					202:207	abrupt hepatocyte injury	184:207	abrupt hepatocyte injury	184:207	Acute liver failure (ALF) is a severe consequence of abrupt hepatocyte injury and has lethal outcomes.
32131144	8	12	from	humans	1619:1624	arg1	treatment					1589:1597	treatment	1589:1597	treatment	1589:1597	In conclusion, the common and specific DEPs in three ALF mouse models at molecular level are identified; and determined a close-to-complete reference map of mouse liver proteins which will be useful for clinical diagnosis and treatment of liver failure in humans.
32131144	8	12	from	humans	1619:1624	arg1	diagnosis					1575:1583	clinical diagnosis	1566:1583	clinical diagnosis	1566:1583	In conclusion, the common and specific DEPs in three ALF mouse models at molecular level are identified; and determined a close-to-complete reference map of mouse liver proteins which will be useful for clinical diagnosis and treatment of liver failure in humans.
32131144	8	13	theme	clinical	1566:1573	arg1	diagnosis					1575:1583	clinical diagnosis	1566:1583	clinical diagnosis	1566:1583	In conclusion, the common and specific DEPs in three ALF mouse models at molecular level are identified; and determined a close-to-complete reference map of mouse liver proteins which will be useful for clinical diagnosis and treatment of liver failure in humans.
32131144	1	14	theme	injury	202:207	arg1	failure					143:149	Acute liver failure	131:149	Acute liver failure (ALF)	131:155	Acute liver failure (ALF) is a severe consequence of abrupt hepatocyte injury and has lethal outcomes.
32131144	1	14	theme	injury	202:207	arg1	consequence					169:179	a severe consequence	160:179	a severe consequence of abrupt hepatocyte injury	160:207	Acute liver failure (ALF) is a severe consequence of abrupt hepatocyte injury and has lethal outcomes.
32131144	7	15	theme	I	1217:1217	arg1	C					1219:1219	I:C	1217:1219	I:C	1217:1219	Among 80,195, and 23 specifically-expressed proteins in poly(I:C)/D-GalN, LPS/D-GalN, and CpG DNA/D-GalN groups, LPS/D-GalN-specific proteins are mostly distributed in the endoplasmic reticulum and more enriched in metabolic pathways, whereas poly (I:C)/D-GalN-specific proteins are mainly in the membrane and CpG DNA/D-GalN-specific proteins are related to the ribosome structural composition.
32131144	7	15	theme	I	1217:1217	arg1	poly					1211:1214	poly	1211:1214	poly (I:C)/D-GalN-specific proteins	1211:1245	Among 80,195, and 23 specifically-expressed proteins in poly(I:C)/D-GalN, LPS/D-GalN, and CpG DNA/D-GalN groups, LPS/D-GalN-specific proteins are mostly distributed in the endoplasmic reticulum and more enriched in metabolic pathways, whereas poly (I:C)/D-GalN-specific proteins are mainly in the membrane and CpG DNA/D-GalN-specific proteins are related to the ribosome structural composition.
32131144	8	16	from	failure	1608:1614	arg1	humans					1619:1624	humans	1619:1624	humans	1619:1624	In conclusion, the common and specific DEPs in three ALF mouse models at molecular level are identified; and determined a close-to-complete reference map of mouse liver proteins which will be useful for clinical diagnosis and treatment of liver failure in humans.
32131144	7	17	theme	poly	1024:1027	arg1	/D-GalN					1033:1039	poly(I:C)/D-GalN	1024:1039	poly(I:C)/D-GalN	1024:1039	Among 80,195, and 23 specifically-expressed proteins in poly(I:C)/D-GalN, LPS/D-GalN, and CpG DNA/D-GalN groups, LPS/D-GalN-specific proteins are mostly distributed in the endoplasmic reticulum and more enriched in metabolic pathways, whereas poly (I:C)/D-GalN-specific proteins are mainly in the membrane and CpG DNA/D-GalN-specific proteins are related to the ribosome structural composition.
32131144	6	18	theme	Gene	822:825	arg1	analyses					836:843	Gene ontology analyses	822:843	Gene ontology analyses	822:843	Gene ontology analyses show that among the common DEPs, ten proteins are involved in immune system process, and 39 proteins in metabolic process.
32131144	7	19	theme	poly	1211:1214	arg1	proteins					1238:1245	poly (I:C)/D-GalN-specific proteins	1211:1245	poly (I:C)/D-GalN-specific proteins	1211:1245	Among 80,195, and 23 specifically-expressed proteins in poly(I:C)/D-GalN, LPS/D-GalN, and CpG DNA/D-GalN groups, LPS/D-GalN-specific proteins are mostly distributed in the endoplasmic reticulum and more enriched in metabolic pathways, whereas poly (I:C)/D-GalN-specific proteins are mainly in the membrane and CpG DNA/D-GalN-specific proteins are related to the ribosome structural composition.
32131144	1	20	theme	liver	137:141	arg1	failure					143:149	Acute liver failure	131:149	Acute liver failure (ALF)	131:155	Acute liver failure (ALF) is a severe consequence of abrupt hepatocyte injury and has lethal outcomes.
32131144	1	20	theme	liver	137:141	arg1	consequence					169:179	a severe consequence	160:179	a severe consequence of abrupt hepatocyte injury	160:207	Acute liver failure (ALF) is a severe consequence of abrupt hepatocyte injury and has lethal outcomes.
32131144	1	20	theme	liver	137:141	arg1	ALF					152:154	ALF	152:154	ALF	152:154	Acute liver failure (ALF) is a severe consequence of abrupt hepatocyte injury and has lethal outcomes.
32131144	4	21	theme	mass	621:624	arg1	analyses					659:666	tandem mass tag based quantitative proteomic analyses	614:666	tandem mass tag based quantitative proteomic analyses of three ALF mouse models	614:692	Here, tandem mass tag based quantitative proteomic analyses of three ALF mouse models are performed.
32131144	8	22	from	level	1446:1450	arg1	DEPs					1402:1405	the common and specific DEPs	1378:1405	the common and specific DEPs in three ALF mouse models at molecular level	1378:1450	In conclusion, the common and specific DEPs in three ALF mouse models at molecular level are identified; and determined a close-to-complete reference map of mouse liver proteins which will be useful for clinical diagnosis and treatment of liver failure in humans.
32131144	8	23	theme	failure	1608:1614	arg1	treatment					1589:1597	treatment	1589:1597	treatment	1589:1597	In conclusion, the common and specific DEPs in three ALF mouse models at molecular level are identified; and determined a close-to-complete reference map of mouse liver proteins which will be useful for clinical diagnosis and treatment of liver failure in humans.
32131144	8	23	theme	failure	1608:1614	arg1	diagnosis					1575:1583	clinical diagnosis	1566:1583	clinical diagnosis	1566:1583	In conclusion, the common and specific DEPs in three ALF mouse models at molecular level are identified; and determined a close-to-complete reference map of mouse liver proteins which will be useful for clinical diagnosis and treatment of liver failure in humans.
32131144	7	24	theme	metabolic	1183:1191	arg1	pathways					1193:1200	metabolic pathways	1183:1200	metabolic pathways	1183:1200	Among 80,195, and 23 specifically-expressed proteins in poly(I:C)/D-GalN, LPS/D-GalN, and CpG DNA/D-GalN groups, LPS/D-GalN-specific proteins are mostly distributed in the endoplasmic reticulum and more enriched in metabolic pathways, whereas poly (I:C)/D-GalN-specific proteins are mainly in the membrane and CpG DNA/D-GalN-specific proteins are related to the ribosome structural composition.
32131144	7	25	theme	CpG	1058:1060	arg1	groups					1073:1078	CpG DNA/D-GalN groups	1058:1078	CpG DNA/D-GalN groups	1058:1078	Among 80,195, and 23 specifically-expressed proteins in poly(I:C)/D-GalN, LPS/D-GalN, and CpG DNA/D-GalN groups, LPS/D-GalN-specific proteins are mostly distributed in the endoplasmic reticulum and more enriched in metabolic pathways, whereas poly (I:C)/D-GalN-specific proteins are mainly in the membrane and CpG DNA/D-GalN-specific proteins are related to the ribosome structural composition.
32131144	2	26	theme	toll-like	240:248	arg1	acid					306:309	polyinosinic-polycytidylic acid	279:309	polyinosinic-polycytidylic acid (poly(I:C))	279:321	Three toll-like receptor agonists, including polyinosinic-polycytidylic acid (poly(I:C)), lipopolysaccharide (LPS), and cytosine-phosphate-guanine (CpG) DNA, cause acute and severe hepatitis, respectively, in D-galactosamine (D-GalN)-sensitized mice.
32131144	2	26	theme	toll-like	240:248	arg1	DNA					387:389	cytosine-phosphate-guanine (CpG) DNA	354:389	cytosine-phosphate-guanine (CpG) DNA	354:389	Three toll-like receptor agonists, including polyinosinic-polycytidylic acid (poly(I:C)), lipopolysaccharide (LPS), and cytosine-phosphate-guanine (CpG) DNA, cause acute and severe hepatitis, respectively, in D-galactosamine (D-GalN)-sensitized mice.
32131144	2	26	theme	toll-like	240:248	arg1	agonists					259:266	Three toll-like receptor agonists	234:266	Three toll-like receptor agonists	234:266	Three toll-like receptor agonists, including polyinosinic-polycytidylic acid (poly(I:C)), lipopolysaccharide (LPS), and cytosine-phosphate-guanine (CpG) DNA, cause acute and severe hepatitis, respectively, in D-galactosamine (D-GalN)-sensitized mice.
32131144	2	26	theme	toll-like	240:248	arg1	lipopolysaccharide					324:341	lipopolysaccharide	324:341	lipopolysaccharide (LPS)	324:347	Three toll-like receptor agonists, including polyinosinic-polycytidylic acid (poly(I:C)), lipopolysaccharide (LPS), and cytosine-phosphate-guanine (CpG) DNA, cause acute and severe hepatitis, respectively, in D-galactosamine (D-GalN)-sensitized mice.
32131144	0	27	from	Analyses	22:29	arg1	Mice					74:77	D-Galactosamine-Sensitized Mice	47:77	D-Galactosamine-Sensitized Mice	47:77	Comparative Proteomic Analyses of the Liver in D-Galactosamine-Sensitized Mice Treated with Different Toll-Like Receptor Agonists.
32131144	0	28	theme	Toll-Like	102:110	arg1	Agonists					121:128	Different Toll-Like Receptor Agonists	92:128	Different Toll-Like Receptor Agonists	92:128	Comparative Proteomic Analyses of the Liver in D-Galactosamine-Sensitized Mice Treated with Different Toll-Like Receptor Agonists.
32131144	2	29	dep	I	317:317	arg1	C					319:319	C	319:319	C	319:319	Three toll-like receptor agonists, including polyinosinic-polycytidylic acid (poly(I:C)), lipopolysaccharide (LPS), and cytosine-phosphate-guanine (CpG) DNA, cause acute and severe hepatitis, respectively, in D-galactosamine (D-GalN)-sensitized mice.
32131144	2	30	theme	-sensitized	467:477	arg1	mice					479:482	D-galactosamine (D-GalN)-sensitized mice	443:482	D-galactosamine (D-GalN)-sensitized mice	443:482	Three toll-like receptor agonists, including polyinosinic-polycytidylic acid (poly(I:C)), lipopolysaccharide (LPS), and cytosine-phosphate-guanine (CpG) DNA, cause acute and severe hepatitis, respectively, in D-galactosamine (D-GalN)-sensitized mice.
32131144	2	31	from	hepatitis	415:423	arg1	mice					479:482	D-galactosamine (D-GalN)-sensitized mice	443:482	D-galactosamine (D-GalN)-sensitized mice	443:482	Three toll-like receptor agonists, including polyinosinic-polycytidylic acid (poly(I:C)), lipopolysaccharide (LPS), and cytosine-phosphate-guanine (CpG) DNA, cause acute and severe hepatitis, respectively, in D-galactosamine (D-GalN)-sensitized mice.
32131144	4	32	theme	ALF	677:679	arg1	models					687:692	three ALF mouse models	671:692	three ALF mouse models	671:692	Here, tandem mass tag based quantitative proteomic analyses of three ALF mouse models are performed.
32131144	7	33	dep	distributed	1121:1131	arg1	whereas					1203:1209	whereas	1203:1209	whereas	1203:1209	Among 80,195, and 23 specifically-expressed proteins in poly(I:C)/D-GalN, LPS/D-GalN, and CpG DNA/D-GalN groups, LPS/D-GalN-specific proteins are mostly distributed in the endoplasmic reticulum and more enriched in metabolic pathways, whereas poly (I:C)/D-GalN-specific proteins are mainly in the membrane and CpG DNA/D-GalN-specific proteins are related to the ribosome structural composition.
32131144	2	34	theme	severe	408:413	arg1	hepatitis					415:423	acute and severe hepatitis	398:423	acute and severe hepatitis	398:423	Three toll-like receptor agonists, including polyinosinic-polycytidylic acid (poly(I:C)), lipopolysaccharide (LPS), and cytosine-phosphate-guanine (CpG) DNA, cause acute and severe hepatitis, respectively, in D-galactosamine (D-GalN)-sensitized mice.
32131144	4	35	theme	based	630:634	arg1	analyses					659:666	tandem mass tag based quantitative proteomic analyses	614:666	tandem mass tag based quantitative proteomic analyses of three ALF mouse models	614:692	Here, tandem mass tag based quantitative proteomic analyses of three ALF mouse models are performed.
32131144	0	36	theme	Comparative	0:10	arg1	Analyses					22:29	Comparative Proteomic Analyses	0:29	Comparative Proteomic Analyses of the Liver in D-Galactosamine-Sensitized Mice	0:77	Comparative Proteomic Analyses of the Liver in D-Galactosamine-Sensitized Mice Treated with Different Toll-Like Receptor Agonists.
32131144	2	37	theme	acute	398:402	arg1	hepatitis					415:423	acute and severe hepatitis	398:423	acute and severe hepatitis	398:423	Three toll-like receptor agonists, including polyinosinic-polycytidylic acid (poly(I:C)), lipopolysaccharide (LPS), and cytosine-phosphate-guanine (CpG) DNA, cause acute and severe hepatitis, respectively, in D-galactosamine (D-GalN)-sensitized mice.
32131144	2	38	theme	receptor	250:257	arg1	acid					306:309	polyinosinic-polycytidylic acid	279:309	polyinosinic-polycytidylic acid (poly(I:C))	279:321	Three toll-like receptor agonists, including polyinosinic-polycytidylic acid (poly(I:C)), lipopolysaccharide (LPS), and cytosine-phosphate-guanine (CpG) DNA, cause acute and severe hepatitis, respectively, in D-galactosamine (D-GalN)-sensitized mice.
32131144	2	38	theme	receptor	250:257	arg1	DNA					387:389	cytosine-phosphate-guanine (CpG) DNA	354:389	cytosine-phosphate-guanine (CpG) DNA	354:389	Three toll-like receptor agonists, including polyinosinic-polycytidylic acid (poly(I:C)), lipopolysaccharide (LPS), and cytosine-phosphate-guanine (CpG) DNA, cause acute and severe hepatitis, respectively, in D-galactosamine (D-GalN)-sensitized mice.
32131144	2	38	theme	receptor	250:257	arg1	agonists					259:266	Three toll-like receptor agonists	234:266	Three toll-like receptor agonists	234:266	Three toll-like receptor agonists, including polyinosinic-polycytidylic acid (poly(I:C)), lipopolysaccharide (LPS), and cytosine-phosphate-guanine (CpG) DNA, cause acute and severe hepatitis, respectively, in D-galactosamine (D-GalN)-sensitized mice.
32131144	2	38	theme	receptor	250:257	arg1	lipopolysaccharide					324:341	lipopolysaccharide	324:341	lipopolysaccharide (LPS)	324:347	Three toll-like receptor agonists, including polyinosinic-polycytidylic acid (poly(I:C)), lipopolysaccharide (LPS), and cytosine-phosphate-guanine (CpG) DNA, cause acute and severe hepatitis, respectively, in D-galactosamine (D-GalN)-sensitized mice.
32131144	4	39	theme	proteomic	649:657	arg1	analyses					659:666	tandem mass tag based quantitative proteomic analyses	614:666	tandem mass tag based quantitative proteomic analyses of three ALF mouse models	614:692	Here, tandem mass tag based quantitative proteomic analyses of three ALF mouse models are performed.
32131144	6	40	theme	system	914:919	arg1	process					921:927	immune system process	907:927	immune system process	907:927	Gene ontology analyses show that among the common DEPs, ten proteins are involved in immune system process, and 39 proteins in metabolic process.
32131144	3	41	dep	models	536:541	arg1	/D-GalN					565:571	poly(I:C)/D-GalN	556:571	poly(I:C)/D-GalN	556:571	However, the molecular differences among three ALF models (LPS/D-GalN, poly(I:C)/D-GalN, and CpG DNA/D-GalN), are unclear.
32131144	3	41	dep	models	536:541	arg1	LPS/D-GalN					544:553	LPS/D-GalN	544:553	LPS/D-GalN	544:553	However, the molecular differences among three ALF models (LPS/D-GalN, poly(I:C)/D-GalN, and CpG DNA/D-GalN), are unclear.
32131144	3	41	dep	models	536:541	arg1	models					536:541	three ALF models	526:541	three ALF models (LPS/D-GalN, poly(I:C)/D-GalN, and CpG DNA/D-GalN)	526:592	However, the molecular differences among three ALF models (LPS/D-GalN, poly(I:C)/D-GalN, and CpG DNA/D-GalN), are unclear.
32131144	3	41	dep	models	536:541	arg1	DNA/D-GalN					582:591	CpG DNA/D-GalN	578:591	CpG DNA/D-GalN	578:591	However, the molecular differences among three ALF models (LPS/D-GalN, poly(I:C)/D-GalN, and CpG DNA/D-GalN), are unclear.
32131144	7	42	theme	specifically-expressed	989:1010	arg1	proteins					1012:1019	80,195, and 23 specifically-expressed proteins	974:1019	80,195, and 23 specifically-expressed proteins in poly(I:C)/D-GalN, LPS/D-GalN, and CpG DNA/D-GalN groups	974:1078	Among 80,195, and 23 specifically-expressed proteins in poly(I:C)/D-GalN, LPS/D-GalN, and CpG DNA/D-GalN groups, LPS/D-GalN-specific proteins are mostly distributed in the endoplasmic reticulum and more enriched in metabolic pathways, whereas poly (I:C)/D-GalN-specific proteins are mainly in the membrane and CpG DNA/D-GalN-specific proteins are related to the ribosome structural composition.
32131144	7	43	theme	structural	1339:1348	arg1	composition					1350:1360	the ribosome structural composition	1326:1360	the ribosome structural composition	1326:1360	Among 80,195, and 23 specifically-expressed proteins in poly(I:C)/D-GalN, LPS/D-GalN, and CpG DNA/D-GalN groups, LPS/D-GalN-specific proteins are mostly distributed in the endoplasmic reticulum and more enriched in metabolic pathways, whereas poly (I:C)/D-GalN-specific proteins are mainly in the membrane and CpG DNA/D-GalN-specific proteins are related to the ribosome structural composition.
32131144	4	44	theme	models	687:692	arg1	analyses					659:666	tandem mass tag based quantitative proteomic analyses	614:666	tandem mass tag based quantitative proteomic analyses of three ALF mouse models	614:692	Here, tandem mass tag based quantitative proteomic analyses of three ALF mouse models are performed.
32131144	1	45	theme	lethal	217:222	arg1	outcomes					224:231	lethal outcomes	217:231	lethal outcomes	217:231	Acute liver failure (ALF) is a severe consequence of abrupt hepatocyte injury and has lethal outcomes.
32131144	8	46	theme	mouse	1420:1424	arg1	models					1426:1431	three ALF mouse models	1410:1431	three ALF mouse models	1410:1431	In conclusion, the common and specific DEPs in three ALF mouse models at molecular level are identified; and determined a close-to-complete reference map of mouse liver proteins which will be useful for clinical diagnosis and treatment of liver failure in humans.
32131144	3	47	theme	CpG	578:580	arg1	models					536:541	three ALF models	526:541	three ALF models (LPS/D-GalN, poly(I:C)/D-GalN, and CpG DNA/D-GalN)	526:592	However, the molecular differences among three ALF models (LPS/D-GalN, poly(I:C)/D-GalN, and CpG DNA/D-GalN), are unclear.
32131144	3	47	theme	CpG	578:580	arg1	DNA/D-GalN					582:591	CpG DNA/D-GalN	578:591	CpG DNA/D-GalN	578:591	However, the molecular differences among three ALF models (LPS/D-GalN, poly(I:C)/D-GalN, and CpG DNA/D-GalN), are unclear.
32131144	7	48	theme	LPS/D-GalN-specific	1081:1099	arg1	proteins					1101:1108	LPS/D-GalN-specific proteins	1081:1108	LPS/D-GalN-specific proteins	1081:1108	Among 80,195, and 23 specifically-expressed proteins in poly(I:C)/D-GalN, LPS/D-GalN, and CpG DNA/D-GalN groups, LPS/D-GalN-specific proteins are mostly distributed in the endoplasmic reticulum and more enriched in metabolic pathways, whereas poly (I:C)/D-GalN-specific proteins are mainly in the membrane and CpG DNA/D-GalN-specific proteins are related to the ribosome structural composition.
32131144	8	49	theme	reference	1503:1511	arg1	map					1513:1515	a close-to-complete reference map	1483:1515	a close-to-complete reference map of mouse liver proteins which will be useful for clinical diagnosis and treatment of liver failure in humans	1483:1624	In conclusion, the common and specific DEPs in three ALF mouse models at molecular level are identified; and determined a close-to-complete reference map of mouse liver proteins which will be useful for clinical diagnosis and treatment of liver failure in humans.
32131144	8	49	theme	reference	1503:1511	arg1	useful					1555:1560	useful	1555:1560	useful	1555:1560	In conclusion, the common and specific DEPs in three ALF mouse models at molecular level are identified; and determined a close-to-complete reference map of mouse liver proteins which will be useful for clinical diagnosis and treatment of liver failure in humans.
32131144	7	50	theme	CpG	1278:1280	arg1	proteins					1302:1309	the membrane and CpG DNA/D-GalN-specific proteins	1261:1309	proteins	1302:1309	Among 80,195, and 23 specifically-expressed proteins in poly(I:C)/D-GalN, LPS/D-GalN, and CpG DNA/D-GalN groups, LPS/D-GalN-specific proteins are mostly distributed in the endoplasmic reticulum and more enriched in metabolic pathways, whereas poly (I:C)/D-GalN-specific proteins are mainly in the membrane and CpG DNA/D-GalN-specific proteins are related to the ribosome structural composition.
32131144	2	51	theme	polyinosinic-polycytidylic	279:304	arg1	acid					306:309	polyinosinic-polycytidylic acid	279:309	polyinosinic-polycytidylic acid (poly(I:C))	279:321	Three toll-like receptor agonists, including polyinosinic-polycytidylic acid (poly(I:C)), lipopolysaccharide (LPS), and cytosine-phosphate-guanine (CpG) DNA, cause acute and severe hepatitis, respectively, in D-galactosamine (D-GalN)-sensitized mice.
32131144	8	52	theme	liver	1526:1530	arg1	proteins					1532:1539	mouse liver proteins	1520:1539	mouse liver proteins	1520:1539	In conclusion, the common and specific DEPs in three ALF mouse models at molecular level are identified; and determined a close-to-complete reference map of mouse liver proteins which will be useful for clinical diagnosis and treatment of liver failure in humans.
32131144	1	53	theme	severe	162:167	arg1	failure					143:149	Acute liver failure	131:149	Acute liver failure (ALF)	131:155	Acute liver failure (ALF) is a severe consequence of abrupt hepatocyte injury and has lethal outcomes.
32131144	1	53	theme	severe	162:167	arg1	consequence					169:179	a severe consequence	160:179	a severe consequence of abrupt hepatocyte injury	160:207	Acute liver failure (ALF) is a severe consequence of abrupt hepatocyte injury and has lethal outcomes.
32131144	8	54	from	treatment	1589:1597	arg1	humans					1619:1624	humans	1619:1624	humans	1619:1624	In conclusion, the common and specific DEPs in three ALF mouse models at molecular level are identified; and determined a close-to-complete reference map of mouse liver proteins which will be useful for clinical diagnosis and treatment of liver failure in humans.
32131144	8	55	from	diagnosis	1575:1583	arg1	humans					1619:1624	humans	1619:1624	humans	1619:1624	In conclusion, the common and specific DEPs in three ALF mouse models at molecular level are identified; and determined a close-to-complete reference map of mouse liver proteins which will be useful for clinical diagnosis and treatment of liver failure in humans.
32131144	1	56	theme	hepatocyte	191:200	arg1	injury					202:207	abrupt hepatocyte injury	184:207	abrupt hepatocyte injury	184:207	Acute liver failure (ALF) is a severe consequence of abrupt hepatocyte injury and has lethal outcomes.
32131144	6	57	theme	ontology	827:834	arg1	analyses					836:843	Gene ontology analyses	822:843	Gene ontology analyses	822:843	Gene ontology analyses show that among the common DEPs, ten proteins are involved in immune system process, and 39 proteins in metabolic process.
32131144	1	58	theme	Acute	131:135	arg1	failure					143:149	Acute liver failure	131:149	Acute liver failure (ALF)	131:155	Acute liver failure (ALF) is a severe consequence of abrupt hepatocyte injury and has lethal outcomes.
32131144	1	58	theme	Acute	131:135	arg1	consequence					169:179	a severe consequence	160:179	a severe consequence of abrupt hepatocyte injury	160:207	Acute liver failure (ALF) is a severe consequence of abrupt hepatocyte injury and has lethal outcomes.
32131144	1	58	theme	Acute	131:135	arg1	ALF					152:154	ALF	152:154	ALF	152:154	Acute liver failure (ALF) is a severe consequence of abrupt hepatocyte injury and has lethal outcomes.
32131144	3	59	theme	I	561:561	arg1	C					563:563	I:C	561:563	I:C	561:563	However, the molecular differences among three ALF models (LPS/D-GalN, poly(I:C)/D-GalN, and CpG DNA/D-GalN), are unclear.
32131144	3	59	theme	I	561:561	arg1	poly					556:559	poly	556:559	poly(I:C)/D-GalN	556:571	However, the molecular differences among three ALF models (LPS/D-GalN, poly(I:C)/D-GalN, and CpG DNA/D-GalN), are unclear.
32131144	4	60	theme	tandem	614:619	arg1	analyses					659:666	tandem mass tag based quantitative proteomic analyses	614:666	tandem mass tag based quantitative proteomic analyses of three ALF mouse models	614:692	Here, tandem mass tag based quantitative proteomic analyses of three ALF mouse models are performed.
32131144	3	61	theme	poly	556:559	arg1	/D-GalN					565:571	poly(I:C)/D-GalN	556:571	poly(I:C)/D-GalN	556:571	However, the molecular differences among three ALF models (LPS/D-GalN, poly(I:C)/D-GalN, and CpG DNA/D-GalN), are unclear.
32131144	3	61	theme	poly	556:559	arg1	models					536:541	three ALF models	526:541	three ALF models (LPS/D-GalN, poly(I:C)/D-GalN, and CpG DNA/D-GalN)	526:592	However, the molecular differences among three ALF models (LPS/D-GalN, poly(I:C)/D-GalN, and CpG DNA/D-GalN), are unclear.
32131144	8	62	theme	liver	1602:1606	arg1	failure					1608:1614	liver failure	1602:1614	liver failure in humans	1602:1624	In conclusion, the common and specific DEPs in three ALF mouse models at molecular level are identified; and determined a close-to-complete reference map of mouse liver proteins which will be useful for clinical diagnosis and treatment of liver failure in humans.
32131144	0	63	theme	Different	92:100	arg1	Agonists					121:128	Different Toll-Like Receptor Agonists	92:128	Different Toll-Like Receptor Agonists	92:128	Comparative Proteomic Analyses of the Liver in D-Galactosamine-Sensitized Mice Treated with Different Toll-Like Receptor Agonists.
32131144	5	64	theme	ALF	785:787	arg1	groups					789:794	three ALF groups	779:794	three ALF groups	779:794	52 common differentially expressed proteins (DEPs) are identified, in three ALF groups, compared to the control.
32131144	7	65	from	proteins	1012:1019	arg1	/D-GalN					1033:1039	poly(I:C)/D-GalN	1024:1039	poly(I:C)/D-GalN	1024:1039	Among 80,195, and 23 specifically-expressed proteins in poly(I:C)/D-GalN, LPS/D-GalN, and CpG DNA/D-GalN groups, LPS/D-GalN-specific proteins are mostly distributed in the endoplasmic reticulum and more enriched in metabolic pathways, whereas poly (I:C)/D-GalN-specific proteins are mainly in the membrane and CpG DNA/D-GalN-specific proteins are related to the ribosome structural composition.
32131144	7	65	from	proteins	1012:1019	arg1	groups					1073:1078	CpG DNA/D-GalN groups	1058:1078	CpG DNA/D-GalN groups	1058:1078	Among 80,195, and 23 specifically-expressed proteins in poly(I:C)/D-GalN, LPS/D-GalN, and CpG DNA/D-GalN groups, LPS/D-GalN-specific proteins are mostly distributed in the endoplasmic reticulum and more enriched in metabolic pathways, whereas poly (I:C)/D-GalN-specific proteins are mainly in the membrane and CpG DNA/D-GalN-specific proteins are related to the ribosome structural composition.
32131144	7	65	from	proteins	1012:1019	arg1	LPS/D-GalN					1042:1051	LPS/D-GalN	1042:1051	LPS/D-GalN	1042:1051	Among 80,195, and 23 specifically-expressed proteins in poly(I:C)/D-GalN, LPS/D-GalN, and CpG DNA/D-GalN groups, LPS/D-GalN-specific proteins are mostly distributed in the endoplasmic reticulum and more enriched in metabolic pathways, whereas poly (I:C)/D-GalN-specific proteins are mainly in the membrane and CpG DNA/D-GalN-specific proteins are related to the ribosome structural composition.
32131144	2	66	dep	acid	306:309	arg1	I					317:317	I	317:317	I	317:317	Three toll-like receptor agonists, including polyinosinic-polycytidylic acid (poly(I:C)), lipopolysaccharide (LPS), and cytosine-phosphate-guanine (CpG) DNA, cause acute and severe hepatitis, respectively, in D-galactosamine (D-GalN)-sensitized mice.
32131144	2	66	dep	acid	306:309	arg1	poly					312:315	poly	312:315	poly(I:C)	312:320	Three toll-like receptor agonists, including polyinosinic-polycytidylic acid (poly(I:C)), lipopolysaccharide (LPS), and cytosine-phosphate-guanine (CpG) DNA, cause acute and severe hepatitis, respectively, in D-galactosamine (D-GalN)-sensitized mice.
32131144	0	67	theme	Receptor	112:119	arg1	Agonists					121:128	Different Toll-Like Receptor Agonists	92:128	Different Toll-Like Receptor Agonists	92:128	Comparative Proteomic Analyses of the Liver in D-Galactosamine-Sensitized Mice Treated with Different Toll-Like Receptor Agonists.
32131144	6	68	theme	common	865:870	arg1	DEPs					872:875	the common DEPs	861:875	the common DEPs	861:875	Gene ontology analyses show that among the common DEPs, ten proteins are involved in immune system process, and 39 proteins in metabolic process.
32131144	2	69	theme	cytosine-phosphate-guanine	354:379	arg1	DNA					387:389	cytosine-phosphate-guanine (CpG) DNA	354:389	cytosine-phosphate-guanine (CpG) DNA	354:389	Three toll-like receptor agonists, including polyinosinic-polycytidylic acid (poly(I:C)), lipopolysaccharide (LPS), and cytosine-phosphate-guanine (CpG) DNA, cause acute and severe hepatitis, respectively, in D-galactosamine (D-GalN)-sensitized mice.
32131144	0	70	theme	Proteomic	12:20	arg1	Analyses					22:29	Comparative Proteomic Analyses	0:29	Comparative Proteomic Analyses of the Liver in D-Galactosamine-Sensitized Mice	0:77	Comparative Proteomic Analyses of the Liver in D-Galactosamine-Sensitized Mice Treated with Different Toll-Like Receptor Agonists.
32131144	8	71	theme	common	1382:1387	arg1	DEPs					1402:1405	the common and specific DEPs	1378:1405	the common and specific DEPs in three ALF mouse models at molecular level	1378:1450	In conclusion, the common and specific DEPs in three ALF mouse models at molecular level are identified; and determined a close-to-complete reference map of mouse liver proteins which will be useful for clinical diagnosis and treatment of liver failure in humans.
32131144	4	72	theme	tag	626:628	arg1	analyses					659:666	tandem mass tag based quantitative proteomic analyses	614:666	tandem mass tag based quantitative proteomic analyses of three ALF mouse models	614:692	Here, tandem mass tag based quantitative proteomic analyses of three ALF mouse models are performed.
32131144	5	73	theme	common	712:717	arg1	DEPs					754:757	DEPs	754:757	DEPs	754:757	52 common differentially expressed proteins (DEPs) are identified, in three ALF groups, compared to the control.
32131144	5	73	theme	common	712:717	arg1	proteins					744:751	52 common differentially expressed proteins	709:751	52 common differentially expressed proteins (DEPs)	709:758	52 common differentially expressed proteins (DEPs) are identified, in three ALF groups, compared to the control.
32131144	7	74	theme	endoplasmic	1140:1150	arg1	reticulum					1152:1160	the endoplasmic reticulum	1136:1160	the endoplasmic reticulum	1136:1160	Among 80,195, and 23 specifically-expressed proteins in poly(I:C)/D-GalN, LPS/D-GalN, and CpG DNA/D-GalN groups, LPS/D-GalN-specific proteins are mostly distributed in the endoplasmic reticulum and more enriched in metabolic pathways, whereas poly (I:C)/D-GalN-specific proteins are mainly in the membrane and CpG DNA/D-GalN-specific proteins are related to the ribosome structural composition.
32131144	8	75	theme	specific	1393:1400	arg1	DEPs					1402:1405	the common and specific DEPs	1378:1405	the common and specific DEPs in three ALF mouse models at molecular level	1378:1450	In conclusion, the common and specific DEPs in three ALF mouse models at molecular level are identified; and determined a close-to-complete reference map of mouse liver proteins which will be useful for clinical diagnosis and treatment of liver failure in humans.
32131144	4	76	theme	quantitative	636:647	arg1	analyses					659:666	tandem mass tag based quantitative proteomic analyses	614:666	tandem mass tag based quantitative proteomic analyses of three ALF mouse models	614:692	Here, tandem mass tag based quantitative proteomic analyses of three ALF mouse models are performed.
32131144	0	77	theme	Liver	38:42	arg1	Analyses					22:29	Comparative Proteomic Analyses	0:29	Comparative Proteomic Analyses of the Liver in D-Galactosamine-Sensitized Mice	0:77	Comparative Proteomic Analyses of the Liver in D-Galactosamine-Sensitized Mice Treated with Different Toll-Like Receptor Agonists.
32131144	6	78	theme	immune	907:912	arg1	process					921:927	immune system process	907:927	immune system process	907:927	Gene ontology analyses show that among the common DEPs, ten proteins are involved in immune system process, and 39 proteins in metabolic process.
32131144	2	79	theme	CpG	382:384	arg1	DNA					387:389	cytosine-phosphate-guanine (CpG) DNA	354:389	cytosine-phosphate-guanine (CpG) DNA	354:389	Three toll-like receptor agonists, including polyinosinic-polycytidylic acid (poly(I:C)), lipopolysaccharide (LPS), and cytosine-phosphate-guanine (CpG) DNA, cause acute and severe hepatitis, respectively, in D-galactosamine (D-GalN)-sensitized mice.
32131144	0	80	theme	D-Galactosamine-Sensitized	47:72	arg1	Mice					74:77	D-Galactosamine-Sensitized Mice	47:77	D-Galactosamine-Sensitized Mice	47:77	Comparative Proteomic Analyses of the Liver in D-Galactosamine-Sensitized Mice Treated with Different Toll-Like Receptor Agonists.
32131144	8	81	theme	ALF	1416:1418	arg1	models					1426:1431	three ALF mouse models	1410:1431	three ALF mouse models	1410:1431	In conclusion, the common and specific DEPs in three ALF mouse models at molecular level are identified; and determined a close-to-complete reference map of mouse liver proteins which will be useful for clinical diagnosis and treatment of liver failure in humans.
32131144	1	82	contain	has	213:215	arg1	failure					143:149	Acute liver failure	131:149	Acute liver failure (ALF)	131:155	Acute liver failure (ALF) is a severe consequence of abrupt hepatocyte injury and has lethal outcomes.
32131144	1	82	contain	has	213:215	arg1	ALF					152:154	ALF	152:154	ALF	152:154	Acute liver failure (ALF) is a severe consequence of abrupt hepatocyte injury and has lethal outcomes.
32131144	1	82	contain	has	213:215	arg2	outcomes					224:231	lethal outcomes	217:231	lethal outcomes	217:231	Acute liver failure (ALF) is a severe consequence of abrupt hepatocyte injury and has lethal outcomes.
32131144	1	82	contain	has	213:215	arg1	consequence					169:179	a severe consequence	160:179	a severe consequence of abrupt hepatocyte injury	160:207	Acute liver failure (ALF) is a severe consequence of abrupt hepatocyte injury and has lethal outcomes.
32131144	4	83	theme	mouse	681:685	arg1	models					687:692	three ALF mouse models	671:692	three ALF mouse models	671:692	Here, tandem mass tag based quantitative proteomic analyses of three ALF mouse models are performed.
32131144	8	84	theme	close-to-complete	1485:1501	arg1	map					1513:1515	a close-to-complete reference map	1483:1515	a close-to-complete reference map of mouse liver proteins which will be useful for clinical diagnosis and treatment of liver failure in humans	1483:1624	In conclusion, the common and specific DEPs in three ALF mouse models at molecular level are identified; and determined a close-to-complete reference map of mouse liver proteins which will be useful for clinical diagnosis and treatment of liver failure in humans.
32131144	8	84	theme	close-to-complete	1485:1501	arg1	useful					1555:1560	useful	1555:1560	useful	1555:1560	In conclusion, the common and specific DEPs in three ALF mouse models at molecular level are identified; and determined a close-to-complete reference map of mouse liver proteins which will be useful for clinical diagnosis and treatment of liver failure in humans.
32131144	7	85	theme	ribosome	1330:1337	arg1	composition					1350:1360	the ribosome structural composition	1326:1360	the ribosome structural composition	1326:1360	Among 80,195, and 23 specifically-expressed proteins in poly(I:C)/D-GalN, LPS/D-GalN, and CpG DNA/D-GalN groups, LPS/D-GalN-specific proteins are mostly distributed in the endoplasmic reticulum and more enriched in metabolic pathways, whereas poly (I:C)/D-GalN-specific proteins are mainly in the membrane and CpG DNA/D-GalN-specific proteins are related to the ribosome structural composition.
32131144	8	86	theme	molecular	1436:1444	arg1	level					1446:1450	molecular level	1436:1450	molecular level	1436:1450	In conclusion, the common and specific DEPs in three ALF mouse models at molecular level are identified; and determined a close-to-complete reference map of mouse liver proteins which will be useful for clinical diagnosis and treatment of liver failure in humans.
32131144	7	87	theme	I	1029:1029	arg1	C					1031:1031	I:C	1029:1031	I:C	1029:1031	Among 80,195, and 23 specifically-expressed proteins in poly(I:C)/D-GalN, LPS/D-GalN, and CpG DNA/D-GalN groups, LPS/D-GalN-specific proteins are mostly distributed in the endoplasmic reticulum and more enriched in metabolic pathways, whereas poly (I:C)/D-GalN-specific proteins are mainly in the membrane and CpG DNA/D-GalN-specific proteins are related to the ribosome structural composition.
32131144	7	87	theme	I	1029:1029	arg1	poly					1024:1027	poly	1024:1027	poly(I:C)/D-GalN	1024:1039	Among 80,195, and 23 specifically-expressed proteins in poly(I:C)/D-GalN, LPS/D-GalN, and CpG DNA/D-GalN groups, LPS/D-GalN-specific proteins are mostly distributed in the endoplasmic reticulum and more enriched in metabolic pathways, whereas poly (I:C)/D-GalN-specific proteins are mainly in the membrane and CpG DNA/D-GalN-specific proteins are related to the ribosome structural composition.
32131144	7	88	theme	DNA/D-GalN	1062:1071	arg1	groups					1073:1078	CpG DNA/D-GalN groups	1058:1078	CpG DNA/D-GalN groups	1058:1078	Among 80,195, and 23 specifically-expressed proteins in poly(I:C)/D-GalN, LPS/D-GalN, and CpG DNA/D-GalN groups, LPS/D-GalN-specific proteins are mostly distributed in the endoplasmic reticulum and more enriched in metabolic pathways, whereas poly (I:C)/D-GalN-specific proteins are mainly in the membrane and CpG DNA/D-GalN-specific proteins are related to the ribosome structural composition.
32100703	2	0	theme	Gram-stain-positive	96:114	arg1	bacterium					156:164	An obligately anaerobic, Gram-stain-positive, non-motile and coccoid- or oval-shaped bacterium	71:164	An obligately anaerobic, Gram-stain-positive, non-motile and coccoid- or oval-shaped bacterium	71:164	An obligately anaerobic, Gram-stain-positive, non-motile and coccoid- or oval-shaped bacterium, designated strain KGMB01111T, was isolated from faeces from a healthy Korean.
32100703	2	1	theme	non-motile	117:126	arg1	bacterium					156:164	An obligately anaerobic, Gram-stain-positive, non-motile and coccoid- or oval-shaped bacterium	71:164	An obligately anaerobic, Gram-stain-positive, non-motile and coccoid- or oval-shaped bacterium	71:164	An obligately anaerobic, Gram-stain-positive, non-motile and coccoid- or oval-shaped bacterium, designated strain KGMB01111T, was isolated from faeces from a healthy Korean.
32100703	9	2	theme	name	1377:1380	arg1	sp					1400:1401	the name Blautia faecicola sp	1373:1401	the name Blautia faecicola sp	1373:1401	On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, KGMB01111T represents a novel species within the genus Blautia for which the name Blautia faecicola sp.
32100703	3	3	dep	related	331:337	arg1	followed					426:433	followed	426:433	followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa)	426:669	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	7	4	theme	fermentation	1080:1091	arg1	product					1069:1075	The predominant end product	1049:1075	The predominant end product of fermentation produced by KGMB01111T	1049:1114	The predominant end product of fermentation produced by KGMB01111T was acetic acid.
32100703	7	4	theme	fermentation	1080:1091	arg1	acid					1127:1130	acetic acid	1120:1130	acetic acid	1120:1130	The predominant end product of fermentation produced by KGMB01111T was acetic acid.
32100703	4	5	theme	genus	802:806	arg1	Blautia					808:814	the genus Blautia	798:814	the genus Blautia	798:814	Phylogenetic analysis based on the 16S rRNA gene sequences indicated that KGMB01111T formed a separate branch with species in the genus Blautia.
32100703	4	6	theme	separate	766:773	arg1	branch					775:780	a separate branch	764:780	a separate branch with species	764:793	Phylogenetic analysis based on the 16S rRNA gene sequences indicated that KGMB01111T formed a separate branch with species in the genus Blautia.
32100703	3	7	theme	gene	278:281	arg1	sequences					283:291	16S rRNA gene sequences	269:291	16S rRNA gene sequences	269:291	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	6	8	contain	contained	986:994	arg1	KGMB01111T					975:984	KGMB01111T	975:984	KGMB01111T	975:984	KGMB01111T contained meso-diaminopimelic acid in cell-wall peptidoglycan.
32100703	6	8	contain	contained	986:994	arg2	acid					1016:1019	meso-diaminopimelic acid	996:1019	meso-diaminopimelic acid in cell-wall peptidoglycan	996:1046	KGMB01111T contained meso-diaminopimelic acid in cell-wall peptidoglycan.
32100703	3	9	theme	Ruminococcus	342:353	arg1	CCRI-16110T					365:375	Ruminococcus gauveauii CCRI-16110T	342:375	Ruminococcus gauveauii CCRI-16110T (93.9 %)	342:384	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	3	9	theme	Ruminococcus	342:353	arg1	%					383:383	93.9 %	378:383	93.9 %	378:383	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	2	10	theme	anaerobic	85:93	arg1	bacterium					156:164	An obligately anaerobic, Gram-stain-positive, non-motile and coccoid- or oval-shaped bacterium	71:164	An obligately anaerobic, Gram-stain-positive, non-motile and coccoid- or oval-shaped bacterium	71:164	An obligately anaerobic, Gram-stain-positive, non-motile and coccoid- or oval-shaped bacterium, designated strain KGMB01111T, was isolated from faeces from a healthy Korean.
32100703	9	11	dep	characteristics	1283:1297	arg1	basis					1226:1230	basis	1226:1230	basis	1226:1230	On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, KGMB01111T represents a novel species within the genus Blautia for which the name Blautia faecicola sp.
32100703	9	11	dep	characteristics	1283:1297	arg1	the					1222:1224	the	1222:1224	the	1222:1224	On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, KGMB01111T represents a novel species within the genus Blautia for which the name Blautia faecicola sp.
32100703	3	12	theme	producta	485:492	arg1	27340T					499:504	Blautia producta ATCC 27340T	477:504	Blautia producta ATCC 27340T (93.4 %)	477:513	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	3	12	theme	producta	485:492	arg1	%					512:512	93.4 %	507:512	93.4 %	507:512	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	11	13	theme	=KCTC	1453:1457	arg1	KGMB01111T					1441:1450	KGMB01111T	1441:1450	KGMB01111T (=KCTC 15706T=DSM 107827T)	1441:1477	The type strain is KGMB01111T (=KCTC 15706T=DSM 107827T).
32100703	11	13	theme	=KCTC	1453:1457	arg1	107827T					1470:1476	=KCTC 15706T=DSM 107827T	1453:1476	=KCTC 15706T=DSM 107827T	1453:1476	The type strain is KGMB01111T (=KCTC 15706T=DSM 107827T).
32100703	2	14	attach	isolated	201:208	arg1	faeces					215:220	faeces	215:220	faeces	215:220	An obligately anaerobic, Gram-stain-positive, non-motile and coccoid- or oval-shaped bacterium, designated strain KGMB01111T, was isolated from faeces from a healthy Korean.
32100703	2	14	attach	isolated	201:208	arg1	Korean					237:242	a healthy Korean	227:242	a healthy Korean	227:242	An obligately anaerobic, Gram-stain-positive, non-motile and coccoid- or oval-shaped bacterium, designated strain KGMB01111T, was isolated from faeces from a healthy Korean.
32100703	2	14	attach	isolated	201:208	arg2	bacterium					156:164	An obligately anaerobic, Gram-stain-positive, non-motile and coccoid- or oval-shaped bacterium	71:164	An obligately anaerobic, Gram-stain-positive, non-motile and coccoid- or oval-shaped bacterium	71:164	An obligately anaerobic, Gram-stain-positive, non-motile and coccoid- or oval-shaped bacterium, designated strain KGMB01111T, was isolated from faeces from a healthy Korean.
32100703	5	15	theme	major	920:924	arg1	lipids					932:937	the major polar lipids	916:937	the major polar lipids	916:937	The major cellular fatty acids (>10.0 %) were C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA), and the major polar lipids were aminophospholipids and lipids.
32100703	5	15	theme	major	920:924	arg1	aminophospholipids					944:961	aminophospholipids	944:961	aminophospholipids	944:961	The major cellular fatty acids (>10.0 %) were C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA), and the major polar lipids were aminophospholipids and lipids.
32100703	3	16	theme	ATCC	494:497	arg1	27340T					499:504	Blautia producta ATCC 27340T	477:504	Blautia producta ATCC 27340T (93.4 %)	477:513	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	3	16	theme	ATCC	494:497	arg1	%					512:512	93.4 %	507:512	93.4 %	507:512	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	9	17	theme	phylogenetic	1270:1281	arg1	characteristics					1283:1297	the phenotypic, chemotaxonomic and phylogenetic characteristics	1235:1297	the phenotypic, chemotaxonomic and phylogenetic characteristics	1235:1297	On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, KGMB01111T represents a novel species within the genus Blautia for which the name Blautia faecicola sp.
32100703	1	18	attach	isolated	28:35	arg1	faeces					42:47	faeces	42:47	faeces	42:47	nov., isolated from faeces from a healthy human.
32100703	1	18	attach	isolated	28:35	arg2	nov.					22:25	nov.	22:25	nov.	22:25	nov., isolated from faeces from a healthy human.
32100703	1	18	attach	isolated	28:35	arg1	human					64:68	a healthy human	54:68	a healthy human	54:68	nov., isolated from faeces from a healthy human.
32100703	2	19	theme	coccoid-	132:139	arg1	bacterium					156:164	An obligately anaerobic, Gram-stain-positive, non-motile and coccoid- or oval-shaped bacterium	71:164	An obligately anaerobic, Gram-stain-positive, non-motile and coccoid- or oval-shaped bacterium	71:164	An obligately anaerobic, Gram-stain-positive, non-motile and coccoid- or oval-shaped bacterium, designated strain KGMB01111T, was isolated from faeces from a healthy Korean.
32100703	8	20	theme	whole-genome	1146:1157	arg1	sequence					1159:1166	the whole-genome sequence	1142:1166	the whole-genome sequence	1142:1166	Based on the whole-genome sequence, the DNA G+C content of the isolate was 44.7 mol%.
32100703	6	21	theme	cell-wall	1024:1032	arg1	peptidoglycan					1034:1046	cell-wall peptidoglycan	1024:1046	cell-wall peptidoglycan	1024:1046	KGMB01111T contained meso-diaminopimelic acid in cell-wall peptidoglycan.
32100703	7	22	theme	predominant	1053:1063	arg1	product					1069:1075	The predominant end product	1049:1075	The predominant end product of fermentation produced by KGMB01111T	1049:1114	The predominant end product of fermentation produced by KGMB01111T was acetic acid.
32100703	7	22	theme	predominant	1053:1063	arg1	acid					1127:1130	acetic acid	1120:1130	acetic acid	1120:1130	The predominant end product of fermentation produced by KGMB01111T was acetic acid.
32100703	4	23	theme	16S	707:709	arg1	sequences					721:729	the 16S rRNA gene sequences	703:729	the 16S rRNA gene sequences	703:729	Phylogenetic analysis based on the 16S rRNA gene sequences indicated that KGMB01111T formed a separate branch with species in the genus Blautia.
32100703	1	24	theme	healthy	56:62	arg1	human					64:68	a healthy human	54:68	a healthy human	54:68	nov., isolated from faeces from a healthy human.
32100703	0	25	theme	faecicola	8:16	arg1	sp					18:19	Blautia faecicola sp	0:19	Blautia faecicola sp.	0:20	Blautia faecicola sp.
32100703	3	26	theme	family	616:621	arg1	XIVa					665:668	Clostridium rRNA cluster XIVa	640:668	Clostridium rRNA cluster XIVa	640:668	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	3	26	theme	family	616:621	arg1	Lachnospiraceae					623:637	the family Lachnospiraceae	612:637	the family Lachnospiraceae (Clostridium rRNA cluster XIVa)	612:669	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	3	27	dep	Blautia	390:396	arg1	stercoris					398:406	stercoris	398:406	stercoris	398:406	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	5	28	theme	fatty	836:840	arg1	acids					842:846	The major cellular fatty acids	817:846	The major cellular fatty acids (>10.0 %)	817:856	The major cellular fatty acids (>10.0 %) were C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA), and the major polar lipids were aminophospholipids and lipids.
32100703	5	28	theme	fatty	836:840	arg1	%					855:855	>10.0 %	849:855	>10.0 %	849:855	The major cellular fatty acids (>10.0 %) were C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA), and the major polar lipids were aminophospholipids and lipids.
32100703	5	28	theme	fatty	836:840	arg1	C16 					863:866	C16 	863:866	C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA)	863:909	The major cellular fatty acids (>10.0 %) were C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA), and the major polar lipids were aminophospholipids and lipids.
32100703	5	29	theme	polar	926:930	arg1	lipids					932:937	the major polar lipids	916:937	the major polar lipids	916:937	The major cellular fatty acids (>10.0 %) were C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA), and the major polar lipids were aminophospholipids and lipids.
32100703	5	29	theme	polar	926:930	arg1	aminophospholipids					944:961	aminophospholipids	944:961	aminophospholipids	944:961	The major cellular fatty acids (>10.0 %) were C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA), and the major polar lipids were aminophospholipids and lipids.
32100703	9	30	theme	genus	1349:1353	arg1	Blautia					1355:1361	the genus Blautia	1345:1361	the genus Blautia for which the name Blautia faecicola sp	1345:1401	On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, KGMB01111T represents a novel species within the genus Blautia for which the name Blautia faecicola sp.
32100703	0	31	theme	Blautia	0:6	arg1	sp					18:19	Blautia faecicola sp	0:19	Blautia faecicola sp.	0:20	Blautia faecicola sp.
32100703	3	32	theme	cluster	657:663	arg1	XIVa					665:668	Clostridium rRNA cluster XIVa	640:668	Clostridium rRNA cluster XIVa	640:668	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	3	32	theme	cluster	657:663	arg1	Lachnospiraceae					623:637	the family Lachnospiraceae	612:637	the family Lachnospiraceae (Clostridium rRNA cluster XIVa)	612:669	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	2	33	theme	strain	178:183	arg1	KGMB01111T					185:194	strain KGMB01111T	178:194	strain KGMB01111T	178:194	An obligately anaerobic, Gram-stain-positive, non-motile and coccoid- or oval-shaped bacterium, designated strain KGMB01111T, was isolated from faeces from a healthy Korean.
32100703	6	34	theme	meso-diaminopimelic	996:1014	arg1	acid					1016:1019	meso-diaminopimelic acid	996:1019	meso-diaminopimelic acid in cell-wall peptidoglycan	996:1046	KGMB01111T contained meso-diaminopimelic acid in cell-wall peptidoglycan.
32100703	8	35	theme	44.7 mol	1208:1215	arg1	content					1181:1187	the DNA G+C content	1169:1187	the DNA G+C content of the isolate	1169:1202	Based on the whole-genome sequence, the DNA G+C content of the isolate was 44.7 mol%.
32100703	8	35	theme	44.7 mol	1208:1215	arg1	%					1216:1216	44.7 mol%	1208:1216	44.7 mol%	1208:1216	Based on the whole-genome sequence, the DNA G+C content of the isolate was 44.7 mol%.
32100703	3	36	theme	DSM	542:544	arg1	10507T					546:551	Blautia hydrogenotrophica DSM 10507T	516:551	Blautia hydrogenotrophica DSM 10507T (93.1 %)	516:560	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	3	36	theme	DSM	542:544	arg1	%					559:559	93.1 %	554:559	93.1 %	554:559	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	5	37	theme	cellular	827:834	arg1	acids					842:846	The major cellular fatty acids	817:846	The major cellular fatty acids (>10.0 %)	817:856	The major cellular fatty acids (>10.0 %) were C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA), and the major polar lipids were aminophospholipids and lipids.
32100703	5	37	theme	cellular	827:834	arg1	%					855:855	>10.0 %	849:855	>10.0 %	849:855	The major cellular fatty acids (>10.0 %) were C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA), and the major polar lipids were aminophospholipids and lipids.
32100703	5	37	theme	cellular	827:834	arg1	C16 					863:866	C16 	863:866	C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA)	863:909	The major cellular fatty acids (>10.0 %) were C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA), and the major polar lipids were aminophospholipids and lipids.
32100703	4	38	theme	gene	716:719	arg1	sequences					721:729	the 16S rRNA gene sequences	703:729	the 16S rRNA gene sequences	703:729	Phylogenetic analysis based on the 16S rRNA gene sequences indicated that KGMB01111T formed a separate branch with species in the genus Blautia.
32100703	9	39	theme	novel	1324:1328	arg1	species					1330:1336	a novel species	1322:1336	a novel species within the genus Blautia for which the name Blautia faecicola sp	1322:1401	On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, KGMB01111T represents a novel species within the genus Blautia for which the name Blautia faecicola sp.
32100703	3	40	theme	Clostridium	640:650	arg1	XIVa					665:668	Clostridium rRNA cluster XIVa	640:668	Clostridium rRNA cluster XIVa	640:668	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	3	40	theme	Clostridium	640:650	arg1	Lachnospiraceae					623:637	the family Lachnospiraceae	612:637	the family Lachnospiraceae (Clostridium rRNA cluster XIVa)	612:669	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	3	41	theme	16S	269:271	arg1	sequences					283:291	16S rRNA gene sequences	269:291	16S rRNA gene sequences	269:291	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	9	42	theme	Blautia	1382:1388	arg1	sp					1400:1401	the name Blautia faecicola sp	1373:1401	the name Blautia faecicola sp	1373:1401	On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, KGMB01111T represents a novel species within the genus Blautia for which the name Blautia faecicola sp.
32100703	6	43	from	acid	1016:1019	arg1	peptidoglycan					1034:1046	cell-wall peptidoglycan	1024:1046	cell-wall peptidoglycan	1024:1046	KGMB01111T contained meso-diaminopimelic acid in cell-wall peptidoglycan.
32100703	8	44	theme	G+C	1177:1179	arg1	content					1181:1187	the DNA G+C content	1169:1187	the DNA G+C content of the isolate	1169:1202	Based on the whole-genome sequence, the DNA G+C content of the isolate was 44.7 mol%.
32100703	8	44	theme	G+C	1177:1179	arg1	%					1216:1216	44.7 mol%	1208:1216	44.7 mol%	1208:1216	Based on the whole-genome sequence, the DNA G+C content of the isolate was 44.7 mol%.
32100703	3	45	theme	Blautia	390:396	arg1	GAM6-1T					408:414	Blautia stercoris GAM6-1T	390:414	Blautia stercoris GAM6-1T (93.7 %)	390:423	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	3	45	theme	Blautia	390:396	arg1	%					422:422	93.7 %	417:422	93.7 %	417:422	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	3	46	theme	rRNA	652:655	arg1	XIVa					665:668	Clostridium rRNA cluster XIVa	640:668	Clostridium rRNA cluster XIVa	640:668	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	3	46	theme	rRNA	652:655	arg1	Lachnospiraceae					623:637	the family Lachnospiraceae	612:637	the family Lachnospiraceae (Clostridium rRNA cluster XIVa)	612:669	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	3	47	theme	Comparative	245:255	arg1	analysis					257:264	Comparative analysis	245:264	Comparative analysis of 16S rRNA gene sequences	245:291	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	3	48	theme	Blautia	566:572	arg1	29236T					589:594	Blautia coccoides ATCC 29236T	566:594	Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa)	566:669	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	3	48	theme	Blautia	566:572	arg1	%					602:602	93.1 %	597:602	93.1 %	597:602	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	3	49	theme	Blautia	516:522	arg1	10507T					546:551	Blautia hydrogenotrophica DSM 10507T	516:551	Blautia hydrogenotrophica DSM 10507T (93.1 %)	516:560	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	3	49	theme	Blautia	516:522	arg1	%					559:559	93.1 %	554:559	93.1 %	554:559	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	9	50	theme	chemotaxonomic	1251:1264	arg1	characteristics					1283:1297	the phenotypic, chemotaxonomic and phylogenetic characteristics	1235:1297	the phenotypic, chemotaxonomic and phylogenetic characteristics	1235:1297	On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, KGMB01111T represents a novel species within the genus Blautia for which the name Blautia faecicola sp.
32100703	8	51	theme	DNA	1173:1175	arg1	content					1181:1187	the DNA G+C content	1169:1187	the DNA G+C content of the isolate	1169:1202	Based on the whole-genome sequence, the DNA G+C content of the isolate was 44.7 mol%.
32100703	8	51	theme	DNA	1173:1175	arg1	%					1216:1216	44.7 mol%	1208:1216	44.7 mol%	1208:1216	Based on the whole-genome sequence, the DNA G+C content of the isolate was 44.7 mol%.
32100703	3	52	theme	hydrogenotrophica	524:540	arg1	10507T					546:551	Blautia hydrogenotrophica DSM 10507T	516:551	Blautia hydrogenotrophica DSM 10507T (93.1 %)	516:560	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	3	52	theme	hydrogenotrophica	524:540	arg1	%					559:559	93.1 %	554:559	93.1 %	554:559	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	7	53	theme	end	1065:1067	arg1	product					1069:1075	The predominant end product	1049:1075	The predominant end product of fermentation produced by KGMB01111T	1049:1114	The predominant end product of fermentation produced by KGMB01111T was acetic acid.
32100703	7	53	theme	end	1065:1067	arg1	acid					1127:1130	acetic acid	1120:1130	acetic acid	1120:1130	The predominant end product of fermentation produced by KGMB01111T was acetic acid.
32100703	3	54	theme	sequences	283:291	arg1	analysis					257:264	Comparative analysis	245:264	Comparative analysis of 16S rRNA gene sequences	245:291	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	2	55	theme	oval-shaped	144:154	arg1	bacterium					156:164	An obligately anaerobic, Gram-stain-positive, non-motile and coccoid- or oval-shaped bacterium	71:164	An obligately anaerobic, Gram-stain-positive, non-motile and coccoid- or oval-shaped bacterium	71:164	An obligately anaerobic, Gram-stain-positive, non-motile and coccoid- or oval-shaped bacterium, designated strain KGMB01111T, was isolated from faeces from a healthy Korean.
32100703	5	56	theme	cis	883:885	arg1	DMA					906:908	DMA	906:908	DMA	906:908	The major cellular fatty acids (>10.0 %) were C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA), and the major polar lipids were aminophospholipids and lipids.
32100703	5	56	theme	cis	883:885	arg1	acetal					898:903	 1 cis 9 dimethyl acetal	880:903	C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA)	863:909	The major cellular fatty acids (>10.0 %) were C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA), and the major polar lipids were aminophospholipids and lipids.
32100703	3	57	dep	Ruminococcus	342:353	arg1	gauveauii					355:363	gauveauii	355:363	gauveauii	355:363	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	3	58	theme	DSM	457:459	arg1	1787T					461:465	Clostridium nexile DSM 1787T	438:465	Clostridium nexile DSM 1787T (93.5 %)	438:474	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	3	58	theme	DSM	457:459	arg1	%					473:473	93.5 %	468:473	93.5 %	468:473	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	5	59	theme	dimethyl	889:896	arg1	DMA					906:908	DMA	906:908	DMA	906:908	The major cellular fatty acids (>10.0 %) were C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA), and the major polar lipids were aminophospholipids and lipids.
32100703	5	59	theme	dimethyl	889:896	arg1	acetal					898:903	 1 cis 9 dimethyl acetal	880:903	C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA)	863:909	The major cellular fatty acids (>10.0 %) were C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA), and the major polar lipids were aminophospholipids and lipids.
32100703	11	60	theme	type	1426:1429	arg1	KGMB01111T					1441:1450	KGMB01111T	1441:1450	KGMB01111T (=KCTC 15706T=DSM 107827T)	1441:1477	The type strain is KGMB01111T (=KCTC 15706T=DSM 107827T).
32100703	11	60	theme	type	1426:1429	arg1	strain					1431:1436	The type strain	1422:1436	The type strain	1422:1436	The type strain is KGMB01111T (=KCTC 15706T=DSM 107827T).
32100703	5	61	dep	C16 	863:866	arg1	C18 					875:878	C18 	875:878	C18 	875:878	The major cellular fatty acids (>10.0 %) were C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA), and the major polar lipids were aminophospholipids and lipids.
32100703	5	61	dep	C16 	863:866	arg1	acetal					898:903	 1 cis 9 dimethyl acetal	880:903	C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA)	863:909	The major cellular fatty acids (>10.0 %) were C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA), and the major polar lipids were aminophospholipids and lipids.
32100703	5	61	dep	C16 	863:866	arg1	DMA					906:908	DMA	906:908	DMA	906:908	The major cellular fatty acids (>10.0 %) were C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA), and the major polar lipids were aminophospholipids and lipids.
32100703	5	61	dep	C16 	863:866	arg1	 0					868:869	 0	868:869	 0	868:869	The major cellular fatty acids (>10.0 %) were C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA), and the major polar lipids were aminophospholipids and lipids.
32100703	3	62	theme	rRNA	273:276	arg1	sequences					283:291	16S rRNA gene sequences	269:291	16S rRNA gene sequences	269:291	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	5	63	theme	 1	880:881	arg1	DMA					906:908	DMA	906:908	DMA	906:908	The major cellular fatty acids (>10.0 %) were C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA), and the major polar lipids were aminophospholipids and lipids.
32100703	5	63	theme	 1	880:881	arg1	acetal					898:903	 1 cis 9 dimethyl acetal	880:903	C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA)	863:909	The major cellular fatty acids (>10.0 %) were C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA), and the major polar lipids were aminophospholipids and lipids.
32100703	4	64	theme	rRNA	711:714	arg1	sequences					721:729	the 16S rRNA gene sequences	703:729	the 16S rRNA gene sequences	703:729	Phylogenetic analysis based on the 16S rRNA gene sequences indicated that KGMB01111T formed a separate branch with species in the genus Blautia.
32100703	3	65	theme	Clostridium	438:448	arg1	1787T					461:465	Clostridium nexile DSM 1787T	438:465	Clostridium nexile DSM 1787T (93.5 %)	438:474	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	3	65	theme	Clostridium	438:448	arg1	%					473:473	93.5 %	468:473	93.5 %	468:473	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	9	66	theme	faecicola	1390:1398	arg1	sp					1400:1401	the name Blautia faecicola sp	1373:1401	the name Blautia faecicola sp	1373:1401	On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, KGMB01111T represents a novel species within the genus Blautia for which the name Blautia faecicola sp.
32100703	3	67	theme	Blautia	477:483	arg1	27340T					499:504	Blautia producta ATCC 27340T	477:504	Blautia producta ATCC 27340T (93.4 %)	477:513	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	3	67	theme	Blautia	477:483	arg1	%					512:512	93.4 %	507:512	93.4 %	507:512	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	3	68	theme	coccoides	574:582	arg1	29236T					589:594	Blautia coccoides ATCC 29236T	566:594	Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa)	566:669	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	3	68	theme	coccoides	574:582	arg1	%					602:602	93.1 %	597:602	93.1 %	597:602	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	8	69	theme	isolate	1196:1202	arg1	content					1181:1187	the DNA G+C content	1169:1187	the DNA G+C content of the isolate	1169:1202	Based on the whole-genome sequence, the DNA G+C content of the isolate was 44.7 mol%.
32100703	8	69	theme	isolate	1196:1202	arg1	%					1216:1216	44.7 mol%	1208:1216	44.7 mol%	1208:1216	Based on the whole-genome sequence, the DNA G+C content of the isolate was 44.7 mol%.
32100703	11	70	theme	15706T=DSM	1459:1468	arg1	KGMB01111T					1441:1450	KGMB01111T	1441:1450	KGMB01111T (=KCTC 15706T=DSM 107827T)	1441:1477	The type strain is KGMB01111T (=KCTC 15706T=DSM 107827T).
32100703	11	70	theme	15706T=DSM	1459:1468	arg1	107827T					1470:1476	=KCTC 15706T=DSM 107827T	1453:1476	=KCTC 15706T=DSM 107827T	1453:1476	The type strain is KGMB01111T (=KCTC 15706T=DSM 107827T).
32100703	3	71	theme	nexile	450:455	arg1	1787T					461:465	Clostridium nexile DSM 1787T	438:465	Clostridium nexile DSM 1787T (93.5 %)	438:474	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	3	71	theme	nexile	450:455	arg1	%					473:473	93.5 %	468:473	93.5 %	468:473	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	4	72	with	branch	775:780	arg1	species					787:793	species	787:793	species	787:793	Phylogenetic analysis based on the 16S rRNA gene sequences indicated that KGMB01111T formed a separate branch with species in the genus Blautia.
32100703	5	73	theme	major	821:825	arg1	acids					842:846	The major cellular fatty acids	817:846	The major cellular fatty acids (>10.0 %)	817:856	The major cellular fatty acids (>10.0 %) were C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA), and the major polar lipids were aminophospholipids and lipids.
32100703	5	73	theme	major	821:825	arg1	%					855:855	>10.0 %	849:855	>10.0 %	849:855	The major cellular fatty acids (>10.0 %) were C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA), and the major polar lipids were aminophospholipids and lipids.
32100703	5	73	theme	major	821:825	arg1	C16 					863:866	C16 	863:866	C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA)	863:909	The major cellular fatty acids (>10.0 %) were C16 : 0 and C18 : 1 cis 9 dimethyl acetal (DMA), and the major polar lipids were aminophospholipids and lipids.
32100703	3	74	theme	ATCC	584:587	arg1	29236T					589:594	Blautia coccoides ATCC 29236T	566:594	Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa)	566:669	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	3	74	theme	ATCC	584:587	arg1	%					602:602	93.1 %	597:602	93.1 %	597:602	Comparative analysis of 16S rRNA gene sequences indicated that KGMB01111T was closely related to Ruminococcus gauveauii CCRI-16110T (93.9 %) and Blautia stercoris GAM6-1T (93.7 %), followed by Clostridium nexile DSM 1787T (93.5 %), Blautia producta ATCC 27340T (93.4 %), Blautia hydrogenotrophica DSM 10507T (93.1 %) and Blautia coccoides ATCC 29236T (93.1 %) within the family Lachnospiraceae (Clostridium rRNA cluster XIVa).
32100703	2	75	theme	healthy	229:235	arg1	Korean					237:242	a healthy Korean	227:242	a healthy Korean	227:242	An obligately anaerobic, Gram-stain-positive, non-motile and coccoid- or oval-shaped bacterium, designated strain KGMB01111T, was isolated from faeces from a healthy Korean.
32100703	7	76	theme	acetic	1120:1125	arg1	product					1069:1075	The predominant end product	1049:1075	The predominant end product of fermentation produced by KGMB01111T	1049:1114	The predominant end product of fermentation produced by KGMB01111T was acetic acid.
32100703	7	76	theme	acetic	1120:1125	arg1	acid					1127:1130	acetic acid	1120:1130	acetic acid	1120:1130	The predominant end product of fermentation produced by KGMB01111T was acetic acid.
32100703	4	77	theme	Phylogenetic	672:683	arg1	analysis					685:692	Phylogenetic analysis	672:692	Phylogenetic analysis based on the 16S rRNA gene sequences	672:729	Phylogenetic analysis based on the 16S rRNA gene sequences indicated that KGMB01111T formed a separate branch with species in the genus Blautia.
32100703	9	78	theme	phenotypic	1239:1248	arg1	characteristics					1283:1297	the phenotypic, chemotaxonomic and phylogenetic characteristics	1235:1297	the phenotypic, chemotaxonomic and phylogenetic characteristics	1235:1297	On the basis of the phenotypic, chemotaxonomic and phylogenetic characteristics, KGMB01111T represents a novel species within the genus Blautia for which the name Blautia faecicola sp.
34301113	0	0	theme	Different	100:108	arg1	Techniques					121:130	Different Extraction Techniques	100:130	Different Extraction Techniques	100:130	Physicochemical and Biological Properties of Polysaccharides from Dictyophora indusiata Prepared by Different Extraction Techniques.
34301113	3	1	theme	monosaccharide	744:757	arg1	compositions					759:770	monosaccharide compositions	744:770	monosaccharide compositions	744:770	D. indusiata polysaccharides prepared by different extraction techniques possessed similar chemical compositions and monosaccharide compositions, while exhibited different molecular weights (M), apparent viscosities, and molar ratios of constituent monosaccharides.
34301113	2	2	theme	6.48	620:623	arg1	%					615:615	%	615:615	%	615:615	Results revealed that extraction yields of D. indusiata polysaccharides prepared by different extraction techniques ranged from 5.62% to 6.48%.
34301113	6	3	dep	D.	1471:1472	arg1	indusiata					1474:1482	D. indusiata	1471:1482	D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U	1471:1532	Indeed, both DFP-P and DFP-H exhibited much higher in vitro binding properties, including fat, cholesterol, and bile acid binding properties, and lipase inhibitory effects than that of D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U.
34301113	7	4	theme	functional	1708:1717	arg1	industry					1724:1731	the functional food industry	1704:1731	the functional food industry	1704:1731	These findings suggest that the PAE technique has good potential for the preparation of D. indusiata polysaccharides with desirable bioactivities for the application in the functional food industry.
34301113	3	5	theme	constituent	864:874	arg1	monosaccharides					876:890	constituent monosaccharides	864:890	constituent monosaccharides	864:890	D. indusiata polysaccharides prepared by different extraction techniques possessed similar chemical compositions and monosaccharide compositions, while exhibited different molecular weights (M), apparent viscosities, and molar ratios of constituent monosaccharides.
34301113	3	6	theme	chemical	718:725	arg1	compositions					727:738	similar chemical compositions	710:738	similar chemical compositions	710:738	D. indusiata polysaccharides prepared by different extraction techniques possessed similar chemical compositions and monosaccharide compositions, while exhibited different molecular weights (M), apparent viscosities, and molar ratios of constituent monosaccharides.
34301113	4	7	theme	D.	910:911	arg1	polysaccharides					923:937	D. indusiata polysaccharides	910:937	D. indusiata polysaccharides prepared by HWE (DFP-H)	910:961	In particularly, D. indusiata polysaccharides prepared by HWE (DFP-H) had the highest M and apparent viscosity among all DFPs, while D. indusiata polysaccharides extracted by UAE (DFP-U) possessed the lowest M and apparent viscosity.
34301113	6	8	theme	inhibitory	1439:1448	arg1	effects					1450:1456	lipase inhibitory effects	1432:1456	lipase inhibitory effects	1432:1456	Indeed, both DFP-P and DFP-H exhibited much higher in vitro binding properties, including fat, cholesterol, and bile acid binding properties, and lipase inhibitory effects than that of D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U.
34301113	6	8	theme	inhibitory	1439:1448	arg1	fat					1376:1378	fat	1376:1378	fat	1376:1378	Indeed, both DFP-P and DFP-H exhibited much higher in vitro binding properties, including fat, cholesterol, and bile acid binding properties, and lipase inhibitory effects than that of D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U.
34301113	6	8	theme	inhibitory	1439:1448	arg1	properties					1416:1425	bile acid binding properties	1398:1425	bile acid binding properties	1398:1425	Indeed, both DFP-P and DFP-H exhibited much higher in vitro binding properties, including fat, cholesterol, and bile acid binding properties, and lipase inhibitory effects than that of D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U.
34301113	6	8	theme	inhibitory	1439:1448	arg1	cholesterol					1381:1391	cholesterol	1381:1391	cholesterol	1381:1391	Indeed, both DFP-P and DFP-H exhibited much higher in vitro binding properties, including fat, cholesterol, and bile acid binding properties, and lipase inhibitory effects than that of D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U.
34301113	3	9	theme	monosaccharides	876:890	arg1	weights					809:815	different molecular weights	789:815	different molecular weights (M)	789:819	D. indusiata polysaccharides prepared by different extraction techniques possessed similar chemical compositions and monosaccharide compositions, while exhibited different molecular weights (M), apparent viscosities, and molar ratios of constituent monosaccharides.
34301113	3	9	theme	monosaccharides	876:890	arg1	viscosities					831:841	apparent viscosities	822:841	apparent viscosities	822:841	D. indusiata polysaccharides prepared by different extraction techniques possessed similar chemical compositions and monosaccharide compositions, while exhibited different molecular weights (M), apparent viscosities, and molar ratios of constituent monosaccharides.
34301113	3	9	theme	monosaccharides	876:890	arg1	M					818:818	M	818:818	M	818:818	D. indusiata polysaccharides prepared by different extraction techniques possessed similar chemical compositions and monosaccharide compositions, while exhibited different molecular weights (M), apparent viscosities, and molar ratios of constituent monosaccharides.
34301113	3	9	theme	monosaccharides	876:890	arg1	ratios					854:859	molar ratios	848:859	molar ratios of constituent monosaccharides	848:890	D. indusiata polysaccharides prepared by different extraction techniques possessed similar chemical compositions and monosaccharide compositions, while exhibited different molecular weights (M), apparent viscosities, and molar ratios of constituent monosaccharides.
34301113	2	10	theme	indusiata	529:537	arg1	polysaccharides					539:553	D. indusiata polysaccharides	526:553	D. indusiata polysaccharides	526:553	Results revealed that extraction yields of D. indusiata polysaccharides prepared by different extraction techniques ranged from 5.62% to 6.48%.
34301113	4	11	dep	D.	1026:1027	arg1	indusiata					1029:1037	D. indusiata	1026:1037	D. indusiata polysaccharides extracted by UAE (DFP-U)	1026:1078	In particularly, D. indusiata polysaccharides prepared by HWE (DFP-H) had the highest M and apparent viscosity among all DFPs, while D. indusiata polysaccharides extracted by UAE (DFP-U) possessed the lowest M and apparent viscosity.
34301113	1	12	theme	physicochemical	426:440	arg1	properties					457:466	their physicochemical and biological properties	420:466	their physicochemical and biological properties	420:466	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	7	13	contain	has	1581:1583	arg1	technique					1571:1579	the PAE technique	1563:1579	the PAE technique	1563:1579	These findings suggest that the PAE technique has good potential for the preparation of D. indusiata polysaccharides with desirable bioactivities for the application in the functional food industry.
34301113	7	13	contain	has	1581:1583	arg2	potential					1590:1598	good potential	1585:1598	good potential for the preparation of D. indusiata polysaccharides with desirable bioactivities for the application in the functional food industry	1585:1731	These findings suggest that the PAE technique has good potential for the preparation of D. indusiata polysaccharides with desirable bioactivities for the application in the functional food industry.
34301113	6	14	theme	lipase	1432:1437	arg1	effects					1450:1456	lipase inhibitory effects	1432:1456	lipase inhibitory effects	1432:1456	Indeed, both DFP-P and DFP-H exhibited much higher in vitro binding properties, including fat, cholesterol, and bile acid binding properties, and lipase inhibitory effects than that of D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U.
34301113	6	14	theme	lipase	1432:1437	arg1	fat					1376:1378	fat	1376:1378	fat	1376:1378	Indeed, both DFP-P and DFP-H exhibited much higher in vitro binding properties, including fat, cholesterol, and bile acid binding properties, and lipase inhibitory effects than that of D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U.
34301113	6	14	theme	lipase	1432:1437	arg1	properties					1416:1425	bile acid binding properties	1398:1425	bile acid binding properties	1398:1425	Indeed, both DFP-P and DFP-H exhibited much higher in vitro binding properties, including fat, cholesterol, and bile acid binding properties, and lipase inhibitory effects than that of D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U.
34301113	6	14	theme	lipase	1432:1437	arg1	cholesterol					1381:1391	cholesterol	1381:1391	cholesterol	1381:1391	Indeed, both DFP-P and DFP-H exhibited much higher in vitro binding properties, including fat, cholesterol, and bile acid binding properties, and lipase inhibitory effects than that of D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U.
34301113	1	15	theme	pressurized	268:278	arg1	PAE					301:303	PAE	301:303	PAE	301:303	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	1	15	theme	pressurized	268:278	arg1	extraction					289:298	pressurized assisted extraction	268:298	pressurized assisted extraction (PAE)	268:304	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	0	16	theme	Extraction	110:119	arg1	Techniques					121:130	Different Extraction Techniques	100:130	Different Extraction Techniques	100:130	Physicochemical and Biological Properties of Polysaccharides from Dictyophora indusiata Prepared by Different Extraction Techniques.
34301113	2	17	theme	5.62	611:614	arg1	%					615:615	%	615:615	%	615:615	Results revealed that extraction yields of D. indusiata polysaccharides prepared by different extraction techniques ranged from 5.62% to 6.48%.
34301113	1	18	theme	assisted	280:287	arg1	PAE					301:303	PAE	301:303	PAE	301:303	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	1	18	theme	assisted	280:287	arg1	extraction					289:298	pressurized assisted extraction	268:298	pressurized assisted extraction (PAE)	268:304	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	6	19	theme	binding	1346:1352	arg1	properties					1354:1363	much higher in vitro binding properties	1325:1363	much higher in vitro binding properties	1325:1363	Indeed, both DFP-P and DFP-H exhibited much higher in vitro binding properties, including fat, cholesterol, and bile acid binding properties, and lipase inhibitory effects than that of D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U.
34301113	6	19	theme	binding	1346:1352	arg1	fat					1376:1378	fat	1376:1378	fat	1376:1378	Indeed, both DFP-P and DFP-H exhibited much higher in vitro binding properties, including fat, cholesterol, and bile acid binding properties, and lipase inhibitory effects than that of D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U.
34301113	6	19	theme	binding	1346:1352	arg1	properties					1416:1425	bile acid binding properties	1398:1425	bile acid binding properties	1398:1425	Indeed, both DFP-P and DFP-H exhibited much higher in vitro binding properties, including fat, cholesterol, and bile acid binding properties, and lipase inhibitory effects than that of D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U.
34301113	6	19	theme	binding	1346:1352	arg1	cholesterol					1381:1391	cholesterol	1381:1391	cholesterol	1381:1391	Indeed, both DFP-P and DFP-H exhibited much higher in vitro binding properties, including fat, cholesterol, and bile acid binding properties, and lipase inhibitory effects than that of D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U.
34301113	1	20	theme	biological	446:455	arg1	properties					457:466	their physicochemical and biological properties	420:466	their physicochemical and biological properties	420:466	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	7	21	theme	food	1719:1722	arg1	industry					1724:1731	the functional food industry	1704:1731	the functional food industry	1704:1731	These findings suggest that the PAE technique has good potential for the preparation of D. indusiata polysaccharides with desirable bioactivities for the application in the functional food industry.
34301113	1	22	theme	traditional	191:201	arg1	HWE					225:227	HWE	225:227	HWE	225:227	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	1	22	theme	traditional	191:201	arg1	extraction					213:222	traditional hot water extraction	191:222	traditional hot water extraction (HWE)	191:228	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	4	23	theme	apparent	985:992	arg1	viscosity					994:1002	apparent viscosity	985:1002	apparent viscosity	985:1002	In particularly, D. indusiata polysaccharides prepared by HWE (DFP-H) had the highest M and apparent viscosity among all DFPs, while D. indusiata polysaccharides extracted by UAE (DFP-U) possessed the lowest M and apparent viscosity.
34301113	2	24	dep	%	615:615	arg1	to					617:618	to	617:618	to	617:618	Results revealed that extraction yields of D. indusiata polysaccharides prepared by different extraction techniques ranged from 5.62% to 6.48%.
34301113	7	25	theme	PAE	1567:1569	arg1	technique					1571:1579	the PAE technique	1563:1579	the PAE technique	1563:1579	These findings suggest that the PAE technique has good potential for the preparation of D. indusiata polysaccharides with desirable bioactivities for the application in the functional food industry.
34301113	6	26	theme	acid	1403:1406	arg1	properties					1416:1425	bile acid binding properties	1398:1425	bile acid binding properties	1398:1425	Indeed, both DFP-P and DFP-H exhibited much higher in vitro binding properties, including fat, cholesterol, and bile acid binding properties, and lipase inhibitory effects than that of D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U.
34301113	1	27	theme	hot	203:205	arg1	HWE					225:227	HWE	225:227	HWE	225:227	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	1	27	theme	hot	203:205	arg1	extraction					213:222	traditional hot water extraction	191:222	traditional hot water extraction (HWE)	191:228	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	3	28	theme	different	668:676	arg1	techniques					689:698	different extraction techniques	668:698	different extraction techniques	668:698	D. indusiata polysaccharides prepared by different extraction techniques possessed similar chemical compositions and monosaccharide compositions, while exhibited different molecular weights (M), apparent viscosities, and molar ratios of constituent monosaccharides.
34301113	1	29	theme	extract	362:368	arg1	polysaccharides					392:406	extract Dictyophora indusiata polysaccharides	362:406	extract Dictyophora indusiata polysaccharides (DFPs)	362:413	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	1	29	theme	extract	362:368	arg1	DFPs					409:412	DFPs	409:412	DFPs	409:412	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	5	30	dep	in	1144:1145	arg1	vitro					1147:1151	vitro	1147:1151	vitro	1147:1151	In addition, the in vitro antioxidant effects of D. indusiata polysaccharides prepared by PAE (DFP-P) and DFP-U were significantly higher than that of others.
34301113	1	31	theme	water	207:211	arg1	HWE					225:227	HWE	225:227	HWE	225:227	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	1	31	theme	water	207:211	arg1	extraction					213:222	traditional hot water extraction	191:222	traditional hot water extraction (HWE)	191:228	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	0	32	theme	Physicochemical	0:14	arg1	Properties					31:40	Physicochemical and Biological Properties	0:40	Physicochemical and Biological Properties of Polysaccharides from Dictyophora indusiata	0:86	Physicochemical and Biological Properties of Polysaccharides from Dictyophora indusiata Prepared by Different Extraction Techniques.
34301113	5	33	theme	indusiata	1179:1187	arg1	polysaccharides					1189:1203	D. indusiata polysaccharides	1176:1203	D. indusiata polysaccharides prepared by PAE (DFP-P) and DFP-U	1176:1237	In addition, the in vitro antioxidant effects of D. indusiata polysaccharides prepared by PAE (DFP-P) and DFP-U were significantly higher than that of others.
34301113	7	34	from	application	1689:1699	arg1	industry					1724:1731	the functional food industry	1704:1731	the functional food industry	1704:1731	These findings suggest that the PAE technique has good potential for the preparation of D. indusiata polysaccharides with desirable bioactivities for the application in the functional food industry.
34301113	7	35	theme	polysaccharides	1636:1650	arg1	preparation					1608:1618	the preparation	1604:1618	the preparation of D. indusiata polysaccharides with desirable bioactivities for the application in the functional food industry	1604:1731	These findings suggest that the PAE technique has good potential for the preparation of D. indusiata polysaccharides with desirable bioactivities for the application in the functional food industry.
34301113	3	36	contain	possessed	700:708	arg1	polysaccharides					640:654	D. indusiata polysaccharides	627:654	D. indusiata polysaccharides prepared by different extraction techniques	627:698	D. indusiata polysaccharides prepared by different extraction techniques possessed similar chemical compositions and monosaccharide compositions, while exhibited different molecular weights (M), apparent viscosities, and molar ratios of constituent monosaccharides.
34301113	3	36	contain	possessed	700:708	arg2	compositions					759:770	monosaccharide compositions	744:770	monosaccharide compositions	744:770	D. indusiata polysaccharides prepared by different extraction techniques possessed similar chemical compositions and monosaccharide compositions, while exhibited different molecular weights (M), apparent viscosities, and molar ratios of constituent monosaccharides.
34301113	3	36	contain	possessed	700:708	arg2	compositions					727:738	similar chemical compositions	710:738	similar chemical compositions	710:738	D. indusiata polysaccharides prepared by different extraction techniques possessed similar chemical compositions and monosaccharide compositions, while exhibited different molecular weights (M), apparent viscosities, and molar ratios of constituent monosaccharides.
34301113	5	37	theme	polysaccharides	1189:1203	arg1	higher					1258:1263	higher	1258:1263	higher	1258:1263	In addition, the in vitro antioxidant effects of D. indusiata polysaccharides prepared by PAE (DFP-P) and DFP-U were significantly higher than that of others.
34301113	5	37	theme	polysaccharides	1189:1203	arg1	effects					1165:1171	the in vitro antioxidant effects	1140:1171	the in vitro antioxidant effects of D. indusiata polysaccharides prepared by PAE (DFP-P) and DFP-U	1140:1237	In addition, the in vitro antioxidant effects of D. indusiata polysaccharides prepared by PAE (DFP-P) and DFP-U were significantly higher than that of others.
34301113	2	38	theme	different	567:575	arg1	techniques					588:597	different extraction techniques	567:597	different extraction techniques	567:597	Results revealed that extraction yields of D. indusiata polysaccharides prepared by different extraction techniques ranged from 5.62% to 6.48%.
34301113	4	39	theme	D.	1026:1027	arg1	polysaccharides					1039:1053	D. indusiata polysaccharides	1026:1053	D. indusiata polysaccharides extracted by UAE (DFP-U)	1026:1078	In particularly, D. indusiata polysaccharides prepared by HWE (DFP-H) had the highest M and apparent viscosity among all DFPs, while D. indusiata polysaccharides extracted by UAE (DFP-U) possessed the lowest M and apparent viscosity.
34301113	0	40	theme	Biological	20:29	arg1	Properties					31:40	Physicochemical and Biological Properties	0:40	Physicochemical and Biological Properties of Polysaccharides from Dictyophora indusiata	0:86	Physicochemical and Biological Properties of Polysaccharides from Dictyophora indusiata Prepared by Different Extraction Techniques.
34301113	1	41	used	used	354:357	arg2	extraction					331:340	ultrasonic-assisted extraction	311:340	ultrasonic-assisted extraction (UAE)	311:346	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	1	41	used	used	354:357	arg2	extraction					289:298	pressurized assisted extraction	268:298	pressurized assisted extraction (PAE)	268:304	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	1	41	used	used	354:357	arg2	techniques					169:178	different extraction techniques	148:178	different extraction techniques	148:178	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	1	41	used	used	354:357	arg2	extraction					213:222	traditional hot water extraction	191:222	traditional hot water extraction (HWE)	191:228	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	1	41	used	used	354:357	arg2	extraction					250:259	microwave-assisted extraction	231:259	microwave-assisted extraction (MAE)	231:265	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	6	42	theme	higher	1330:1335	arg1	properties					1354:1363	much higher in vitro binding properties	1325:1363	much higher in vitro binding properties	1325:1363	Indeed, both DFP-P and DFP-H exhibited much higher in vitro binding properties, including fat, cholesterol, and bile acid binding properties, and lipase inhibitory effects than that of D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U.
34301113	6	42	theme	higher	1330:1335	arg1	fat					1376:1378	fat	1376:1378	fat	1376:1378	Indeed, both DFP-P and DFP-H exhibited much higher in vitro binding properties, including fat, cholesterol, and bile acid binding properties, and lipase inhibitory effects than that of D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U.
34301113	6	42	theme	higher	1330:1335	arg1	properties					1416:1425	bile acid binding properties	1398:1425	bile acid binding properties	1398:1425	Indeed, both DFP-P and DFP-H exhibited much higher in vitro binding properties, including fat, cholesterol, and bile acid binding properties, and lipase inhibitory effects than that of D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U.
34301113	6	42	theme	higher	1330:1335	arg1	cholesterol					1381:1391	cholesterol	1381:1391	cholesterol	1381:1391	Indeed, both DFP-P and DFP-H exhibited much higher in vitro binding properties, including fat, cholesterol, and bile acid binding properties, and lipase inhibitory effects than that of D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U.
34301113	1	43	theme	Dictyophora	370:380	arg1	polysaccharides					392:406	extract Dictyophora indusiata polysaccharides	362:406	extract Dictyophora indusiata polysaccharides (DFPs)	362:413	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	1	43	theme	Dictyophora	370:380	arg1	DFPs					409:412	DFPs	409:412	DFPs	409:412	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	7	44	theme	D.	1623:1624	arg1	polysaccharides					1636:1650	D. indusiata polysaccharides	1623:1650	D. indusiata polysaccharides with desirable bioactivities for the application in the functional food industry	1623:1731	These findings suggest that the PAE technique has good potential for the preparation of D. indusiata polysaccharides with desirable bioactivities for the application in the functional food industry.
34301113	0	45	theme	Polysaccharides	45:59	arg1	Properties					31:40	Physicochemical and Biological Properties	0:40	Physicochemical and Biological Properties of Polysaccharides from Dictyophora indusiata	0:86	Physicochemical and Biological Properties of Polysaccharides from Dictyophora indusiata Prepared by Different Extraction Techniques.
34301113	3	46	theme	molecular	799:807	arg1	weights					809:815	different molecular weights	789:815	different molecular weights (M)	789:819	D. indusiata polysaccharides prepared by different extraction techniques possessed similar chemical compositions and monosaccharide compositions, while exhibited different molecular weights (M), apparent viscosities, and molar ratios of constituent monosaccharides.
34301113	3	46	theme	molecular	799:807	arg1	M					818:818	M	818:818	M	818:818	D. indusiata polysaccharides prepared by different extraction techniques possessed similar chemical compositions and monosaccharide compositions, while exhibited different molecular weights (M), apparent viscosities, and molar ratios of constituent monosaccharides.
34301113	4	47	theme	highest	971:977	arg1	M					979:979	M	979:979	M	979:979	In particularly, D. indusiata polysaccharides prepared by HWE (DFP-H) had the highest M and apparent viscosity among all DFPs, while D. indusiata polysaccharides extracted by UAE (DFP-U) possessed the lowest M and apparent viscosity.
34301113	1	48	theme	indusiata	382:390	arg1	polysaccharides					392:406	extract Dictyophora indusiata polysaccharides	362:406	extract Dictyophora indusiata polysaccharides (DFPs)	362:413	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	1	48	theme	indusiata	382:390	arg1	DFPs					409:412	DFPs	409:412	DFPs	409:412	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	4	49	contain	had	963:965	arg2	M					979:979	M	979:979	M	979:979	In particularly, D. indusiata polysaccharides prepared by HWE (DFP-H) had the highest M and apparent viscosity among all DFPs, while D. indusiata polysaccharides extracted by UAE (DFP-U) possessed the lowest M and apparent viscosity.
34301113	4	49	contain	had	963:965	arg1	polysaccharides					923:937	D. indusiata polysaccharides	910:937	D. indusiata polysaccharides prepared by HWE (DFP-H)	910:961	In particularly, D. indusiata polysaccharides prepared by HWE (DFP-H) had the highest M and apparent viscosity among all DFPs, while D. indusiata polysaccharides extracted by UAE (DFP-U) possessed the lowest M and apparent viscosity.
34301113	4	49	contain	had	963:965	arg2	viscosity					994:1002	apparent viscosity	985:1002	apparent viscosity	985:1002	In particularly, D. indusiata polysaccharides prepared by HWE (DFP-H) had the highest M and apparent viscosity among all DFPs, while D. indusiata polysaccharides extracted by UAE (DFP-U) possessed the lowest M and apparent viscosity.
34301113	6	50	theme	bile	1398:1401	arg1	properties					1416:1425	bile acid binding properties	1398:1425	bile acid binding properties	1398:1425	Indeed, both DFP-P and DFP-H exhibited much higher in vitro binding properties, including fat, cholesterol, and bile acid binding properties, and lipase inhibitory effects than that of D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U.
34301113	4	51	contain	possessed	1080:1088	arg2	viscosity					1116:1124	apparent viscosity	1107:1124	apparent viscosity	1107:1124	In particularly, D. indusiata polysaccharides prepared by HWE (DFP-H) had the highest M and apparent viscosity among all DFPs, while D. indusiata polysaccharides extracted by UAE (DFP-U) possessed the lowest M and apparent viscosity.
34301113	4	51	contain	possessed	1080:1088	arg2	M					1101:1101	M	1101:1101	M	1101:1101	In particularly, D. indusiata polysaccharides prepared by HWE (DFP-H) had the highest M and apparent viscosity among all DFPs, while D. indusiata polysaccharides extracted by UAE (DFP-U) possessed the lowest M and apparent viscosity.
34301113	4	51	contain	possessed	1080:1088	arg1	polysaccharides					1039:1053	D. indusiata polysaccharides	1026:1053	D. indusiata polysaccharides extracted by UAE (DFP-U)	1026:1078	In particularly, D. indusiata polysaccharides prepared by HWE (DFP-H) had the highest M and apparent viscosity among all DFPs, while D. indusiata polysaccharides extracted by UAE (DFP-U) possessed the lowest M and apparent viscosity.
34301113	2	52	theme	polysaccharides	539:553	arg1	yields					516:521	extraction yields	505:521	extraction yields of D. indusiata polysaccharides prepared by different extraction techniques	505:597	Results revealed that extraction yields of D. indusiata polysaccharides prepared by different extraction techniques ranged from 5.62% to 6.48%.
34301113	7	53	with	polysaccharides	1636:1650	arg1	bioactivities					1667:1679	desirable bioactivities	1657:1679	desirable bioactivities for the application in the functional food industry	1657:1731	These findings suggest that the PAE technique has good potential for the preparation of D. indusiata polysaccharides with desirable bioactivities for the application in the functional food industry.
34301113	6	54	dep	in	1337:1338	arg1	vitro					1340:1344	vitro	1340:1344	vitro	1340:1344	Indeed, both DFP-P and DFP-H exhibited much higher in vitro binding properties, including fat, cholesterol, and bile acid binding properties, and lipase inhibitory effects than that of D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U.
34301113	3	55	theme	apparent	822:829	arg1	viscosities					831:841	apparent viscosities	822:841	apparent viscosities	822:841	D. indusiata polysaccharides prepared by different extraction techniques possessed similar chemical compositions and monosaccharide compositions, while exhibited different molecular weights (M), apparent viscosities, and molar ratios of constituent monosaccharides.
34301113	0	56	from	indusiata	78:86	arg1	Polysaccharides					45:59	Polysaccharides	45:59	Polysaccharides from Dictyophora indusiata	45:86	Physicochemical and Biological Properties of Polysaccharides from Dictyophora indusiata Prepared by Different Extraction Techniques.
34301113	0	56	from	indusiata	78:86	arg1	Properties					31:40	Physicochemical and Biological Properties	0:40	Physicochemical and Biological Properties of Polysaccharides from Dictyophora indusiata	0:86	Physicochemical and Biological Properties of Polysaccharides from Dictyophora indusiata Prepared by Different Extraction Techniques.
34301113	5	57	theme	in	1144:1145	arg1	higher					1258:1263	higher	1258:1263	higher	1258:1263	In addition, the in vitro antioxidant effects of D. indusiata polysaccharides prepared by PAE (DFP-P) and DFP-U were significantly higher than that of others.
34301113	5	57	theme	in	1144:1145	arg1	effects					1165:1171	the in vitro antioxidant effects	1140:1171	the in vitro antioxidant effects of D. indusiata polysaccharides prepared by PAE (DFP-P) and DFP-U	1140:1237	In addition, the in vitro antioxidant effects of D. indusiata polysaccharides prepared by PAE (DFP-P) and DFP-U were significantly higher than that of others.
34301113	1	58	theme	ultrasonic-assisted	311:329	arg1	UAE					343:345	UAE	343:345	UAE	343:345	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	1	58	theme	ultrasonic-assisted	311:329	arg1	extraction					331:340	ultrasonic-assisted extraction	311:340	ultrasonic-assisted extraction (UAE)	311:346	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	6	59	theme	in	1337:1338	arg1	properties					1354:1363	much higher in vitro binding properties	1325:1363	much higher in vitro binding properties	1325:1363	Indeed, both DFP-P and DFP-H exhibited much higher in vitro binding properties, including fat, cholesterol, and bile acid binding properties, and lipase inhibitory effects than that of D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U.
34301113	6	59	theme	in	1337:1338	arg1	fat					1376:1378	fat	1376:1378	fat	1376:1378	Indeed, both DFP-P and DFP-H exhibited much higher in vitro binding properties, including fat, cholesterol, and bile acid binding properties, and lipase inhibitory effects than that of D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U.
34301113	6	59	theme	in	1337:1338	arg1	properties					1416:1425	bile acid binding properties	1398:1425	bile acid binding properties	1398:1425	Indeed, both DFP-P and DFP-H exhibited much higher in vitro binding properties, including fat, cholesterol, and bile acid binding properties, and lipase inhibitory effects than that of D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U.
34301113	6	59	theme	in	1337:1338	arg1	cholesterol					1381:1391	cholesterol	1381:1391	cholesterol	1381:1391	Indeed, both DFP-P and DFP-H exhibited much higher in vitro binding properties, including fat, cholesterol, and bile acid binding properties, and lipase inhibitory effects than that of D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U.
34301113	5	60	theme	antioxidant	1153:1163	arg1	higher					1258:1263	higher	1258:1263	higher	1258:1263	In addition, the in vitro antioxidant effects of D. indusiata polysaccharides prepared by PAE (DFP-P) and DFP-U were significantly higher than that of others.
34301113	5	60	theme	antioxidant	1153:1163	arg1	effects					1165:1171	the in vitro antioxidant effects	1140:1171	the in vitro antioxidant effects of D. indusiata polysaccharides prepared by PAE (DFP-P) and DFP-U	1140:1237	In addition, the in vitro antioxidant effects of D. indusiata polysaccharides prepared by PAE (DFP-P) and DFP-U were significantly higher than that of others.
34301113	5	61	theme	D.	1176:1177	arg1	polysaccharides					1189:1203	D. indusiata polysaccharides	1176:1203	D. indusiata polysaccharides prepared by PAE (DFP-P) and DFP-U	1176:1237	In addition, the in vitro antioxidant effects of D. indusiata polysaccharides prepared by PAE (DFP-P) and DFP-U were significantly higher than that of others.
34301113	3	62	theme	similar	710:716	arg1	compositions					727:738	similar chemical compositions	710:738	similar chemical compositions	710:738	D. indusiata polysaccharides prepared by different extraction techniques possessed similar chemical compositions and monosaccharide compositions, while exhibited different molecular weights (M), apparent viscosities, and molar ratios of constituent monosaccharides.
34301113	3	63	theme	D.	627:628	arg1	polysaccharides					640:654	D. indusiata polysaccharides	627:654	D. indusiata polysaccharides prepared by different extraction techniques	627:698	D. indusiata polysaccharides prepared by different extraction techniques possessed similar chemical compositions and monosaccharide compositions, while exhibited different molecular weights (M), apparent viscosities, and molar ratios of constituent monosaccharides.
34301113	3	64	theme	different	789:797	arg1	weights					809:815	different molecular weights	789:815	different molecular weights (M)	789:819	D. indusiata polysaccharides prepared by different extraction techniques possessed similar chemical compositions and monosaccharide compositions, while exhibited different molecular weights (M), apparent viscosities, and molar ratios of constituent monosaccharides.
34301113	3	64	theme	different	789:797	arg1	M					818:818	M	818:818	M	818:818	D. indusiata polysaccharides prepared by different extraction techniques possessed similar chemical compositions and monosaccharide compositions, while exhibited different molecular weights (M), apparent viscosities, and molar ratios of constituent monosaccharides.
34301113	4	65	theme	lowest	1094:1099	arg1	M					1101:1101	M	1101:1101	M	1101:1101	In particularly, D. indusiata polysaccharides prepared by HWE (DFP-H) had the highest M and apparent viscosity among all DFPs, while D. indusiata polysaccharides extracted by UAE (DFP-U) possessed the lowest M and apparent viscosity.
34301113	1	66	theme	microwave-assisted	231:248	arg1	MAE					262:264	MAE	262:264	MAE	262:264	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	1	66	theme	microwave-assisted	231:248	arg1	extraction					250:259	microwave-assisted extraction	231:259	microwave-assisted extraction (MAE)	231:265	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	2	67	theme	extraction	577:586	arg1	techniques					588:597	different extraction techniques	567:597	different extraction techniques	567:597	Results revealed that extraction yields of D. indusiata polysaccharides prepared by different extraction techniques ranged from 5.62% to 6.48%.
34301113	3	68	theme	extraction	678:687	arg1	techniques					689:698	different extraction techniques	668:698	different extraction techniques	668:698	D. indusiata polysaccharides prepared by different extraction techniques possessed similar chemical compositions and monosaccharide compositions, while exhibited different molecular weights (M), apparent viscosities, and molar ratios of constituent monosaccharides.
34301113	3	69	theme	indusiata	630:638	arg1	polysaccharides					640:654	D. indusiata polysaccharides	627:654	D. indusiata polysaccharides prepared by different extraction techniques	627:698	D. indusiata polysaccharides prepared by different extraction techniques possessed similar chemical compositions and monosaccharide compositions, while exhibited different molecular weights (M), apparent viscosities, and molar ratios of constituent monosaccharides.
34301113	7	70	dep	D.	1623:1624	arg1	indusiata					1626:1634	D. indusiata	1623:1634	D. indusiata polysaccharides with desirable bioactivities for the application in the functional food industry	1623:1731	These findings suggest that the PAE technique has good potential for the preparation of D. indusiata polysaccharides with desirable bioactivities for the application in the functional food industry.
34301113	1	71	theme	different	148:156	arg1	techniques					169:178	different extraction techniques	148:178	different extraction techniques	148:178	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	1	71	theme	different	148:156	arg1	extraction					289:298	pressurized assisted extraction	268:298	pressurized assisted extraction (PAE)	268:304	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	1	71	theme	different	148:156	arg1	extraction					213:222	traditional hot water extraction	191:222	traditional hot water extraction (HWE)	191:228	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	1	71	theme	different	148:156	arg1	extraction					250:259	microwave-assisted extraction	231:259	microwave-assisted extraction (MAE)	231:265	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	1	71	theme	different	148:156	arg1	extraction					331:340	ultrasonic-assisted extraction	311:340	ultrasonic-assisted extraction (UAE)	311:346	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	4	72	theme	apparent	1107:1114	arg1	viscosity					1116:1124	apparent viscosity	1107:1124	apparent viscosity	1107:1124	In particularly, D. indusiata polysaccharides prepared by HWE (DFP-H) had the highest M and apparent viscosity among all DFPs, while D. indusiata polysaccharides extracted by UAE (DFP-U) possessed the lowest M and apparent viscosity.
34301113	3	73	theme	molar	848:852	arg1	ratios					854:859	molar ratios	848:859	molar ratios of constituent monosaccharides	848:890	D. indusiata polysaccharides prepared by different extraction techniques possessed similar chemical compositions and monosaccharide compositions, while exhibited different molecular weights (M), apparent viscosities, and molar ratios of constituent monosaccharides.
34301113	2	74	theme	extraction	505:514	arg1	yields					516:521	extraction yields	505:521	extraction yields of D. indusiata polysaccharides prepared by different extraction techniques	505:597	Results revealed that extraction yields of D. indusiata polysaccharides prepared by different extraction techniques ranged from 5.62% to 6.48%.
34301113	7	75	theme	good	1585:1588	arg1	potential					1590:1598	good potential	1585:1598	good potential for the preparation of D. indusiata polysaccharides with desirable bioactivities for the application in the functional food industry	1585:1731	These findings suggest that the PAE technique has good potential for the preparation of D. indusiata polysaccharides with desirable bioactivities for the application in the functional food industry.
34301113	1	76	theme	extraction	158:167	arg1	techniques					169:178	different extraction techniques	148:178	different extraction techniques	148:178	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	1	76	theme	extraction	158:167	arg1	extraction					289:298	pressurized assisted extraction	268:298	pressurized assisted extraction (PAE)	268:304	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	1	76	theme	extraction	158:167	arg1	extraction					213:222	traditional hot water extraction	191:222	traditional hot water extraction (HWE)	191:228	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	1	76	theme	extraction	158:167	arg1	extraction					250:259	microwave-assisted extraction	231:259	microwave-assisted extraction (MAE)	231:265	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	1	76	theme	extraction	158:167	arg1	extraction					331:340	ultrasonic-assisted extraction	311:340	ultrasonic-assisted extraction (UAE)	311:346	In this study, different extraction techniques, including traditional hot water extraction (HWE), microwave-assisted extraction (MAE), pressurized assisted extraction (PAE), and ultrasonic-assisted extraction (UAE), were used to extract Dictyophora indusiata polysaccharides (DFPs), and their physicochemical and biological properties were compared.
34301113	6	77	theme	D.	1471:1472	arg1	polysaccharides					1484:1498	D. indusiata polysaccharides	1471:1498	D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U	1471:1532	Indeed, both DFP-P and DFP-H exhibited much higher in vitro binding properties, including fat, cholesterol, and bile acid binding properties, and lipase inhibitory effects than that of D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U.
34301113	7	78	theme	desirable	1657:1665	arg1	bioactivities					1667:1679	desirable bioactivities	1657:1679	desirable bioactivities for the application in the functional food industry	1657:1731	These findings suggest that the PAE technique has good potential for the preparation of D. indusiata polysaccharides with desirable bioactivities for the application in the functional food industry.
34301113	0	79	from	Properties	31:40	arg1	indusiata					78:86	indusiata	78:86	indusiata	78:86	Physicochemical and Biological Properties of Polysaccharides from Dictyophora indusiata Prepared by Different Extraction Techniques.
34301113	6	80	theme	binding	1408:1414	arg1	properties					1416:1425	bile acid binding properties	1398:1425	bile acid binding properties	1398:1425	Indeed, both DFP-P and DFP-H exhibited much higher in vitro binding properties, including fat, cholesterol, and bile acid binding properties, and lipase inhibitory effects than that of D. indusiata polysaccharides prepared by MAE (DFP-M) and DFP-U.
34301113	4	81	theme	indusiata	913:921	arg1	polysaccharides					923:937	D. indusiata polysaccharides	910:937	D. indusiata polysaccharides prepared by HWE (DFP-H)	910:961	In particularly, D. indusiata polysaccharides prepared by HWE (DFP-H) had the highest M and apparent viscosity among all DFPs, while D. indusiata polysaccharides extracted by UAE (DFP-U) possessed the lowest M and apparent viscosity.
34301113	2	82	theme	D.	526:527	arg1	polysaccharides					539:553	D. indusiata polysaccharides	526:553	D. indusiata polysaccharides	526:553	Results revealed that extraction yields of D. indusiata polysaccharides prepared by different extraction techniques ranged from 5.62% to 6.48%.
32234114	8	0	theme	strain	887:892	arg1	N7XX-4T					894:900	strain N7XX-4T	887:900	strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T)	887:941	On the basis of differences in phenotypic and genotypic characteristics, strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T) is designated as the type strain of a novel species of the genus Lysinimonas, for which the name Lysinimonas yzui sp.
32234114	8	0	theme	strain	887:892	arg1	strain					969:974	the type strain	960:974	the type strain of a novel species of the genus Lysinimonas, for which the name Lysinimonas yzui sp	960:1058	On the basis of differences in phenotypic and genotypic characteristics, strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T) is designated as the type strain of a novel species of the genus Lysinimonas, for which the name Lysinimonas yzui sp.
32234114	8	0	theme	strain	887:892	arg1	32630T					935:940	=CGMCC 1.16548T=DSM 106791T=JCM 32630T	903:940	=CGMCC 1.16548T=DSM 106791T=JCM 32630T	903:940	On the basis of differences in phenotypic and genotypic characteristics, strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T) is designated as the type strain of a novel species of the genus Lysinimonas, for which the name Lysinimonas yzui sp.
32234114	2	1	theme	PR	311:312	arg1	Province					301:308	Province	301:308	Province	301:308	A yellow-pigmented, Gram-stain-negative, aerobic, non-motile rod shaped, mesophilic bacterium, designated strain N7XX-4T, was isolated from cattail root grown on the mine tailings of Phoenix mountain, Tongling city, Anhui Province (PR China).
32234114	2	1	theme	PR	311:312	arg1	China					314:318	PR China	311:318	PR China	311:318	A yellow-pigmented, Gram-stain-negative, aerobic, non-motile rod shaped, mesophilic bacterium, designated strain N7XX-4T, was isolated from cattail root grown on the mine tailings of Phoenix mountain, Tongling city, Anhui Province (PR China).
32234114	8	2	theme	type	964:967	arg1	N7XX-4T					894:900	strain N7XX-4T	887:900	strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T)	887:941	On the basis of differences in phenotypic and genotypic characteristics, strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T) is designated as the type strain of a novel species of the genus Lysinimonas, for which the name Lysinimonas yzui sp.
32234114	8	2	theme	type	964:967	arg1	strain					969:974	the type strain	960:974	the type strain of a novel species of the genus Lysinimonas, for which the name Lysinimonas yzui sp	960:1058	On the basis of differences in phenotypic and genotypic characteristics, strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T) is designated as the type strain of a novel species of the genus Lysinimonas, for which the name Lysinimonas yzui sp.
32234114	2	3	attach	isolated	205:212	arg1	root					227:230	cattail root	219:230	cattail root grown on the mine tailings of Phoenix mountain, Tongling city, Anhui Province (PR China)	219:319	A yellow-pigmented, Gram-stain-negative, aerobic, non-motile rod shaped, mesophilic bacterium, designated strain N7XX-4T, was isolated from cattail root grown on the mine tailings of Phoenix mountain, Tongling city, Anhui Province (PR China).
32234114	2	3	attach	isolated	205:212	arg2	bacterium					163:171	A yellow-pigmented, Gram-stain-negative, aerobic, non-motile rod shaped, mesophilic bacterium	79:171	A yellow-pigmented, Gram-stain-negative, aerobic, non-motile rod shaped, mesophilic bacterium	79:171	A yellow-pigmented, Gram-stain-negative, aerobic, non-motile rod shaped, mesophilic bacterium, designated strain N7XX-4T, was isolated from cattail root grown on the mine tailings of Phoenix mountain, Tongling city, Anhui Province (PR China).
32234114	8	4	theme	species	987:993	arg1	N7XX-4T					894:900	strain N7XX-4T	887:900	strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T)	887:941	On the basis of differences in phenotypic and genotypic characteristics, strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T) is designated as the type strain of a novel species of the genus Lysinimonas, for which the name Lysinimonas yzui sp.
32234114	8	4	theme	species	987:993	arg1	strain					969:974	the type strain	960:974	the type strain of a novel species of the genus Lysinimonas, for which the name Lysinimonas yzui sp	960:1058	On the basis of differences in phenotypic and genotypic characteristics, strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T) is designated as the type strain of a novel species of the genus Lysinimonas, for which the name Lysinimonas yzui sp.
32234114	4	5	theme	phylogenetic	458:469	arg1	neighbour					471:479	The nearest phylogenetic neighbour	446:479	The nearest phylogenetic neighbour	446:479	The nearest phylogenetic neighbour was Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity).
32234114	4	5	theme	phylogenetic	458:469	arg1	MSL-13T					508:514	Lysinimonas kribbensis MSL-13T	485:514	Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity)	485:557	The nearest phylogenetic neighbour was Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity).
32234114	5	6	theme	whole	592:596	arg1	cells					598:602	whole cells	592:602	whole cells of N7XX-4T	592:613	The most abundant fatty acid in whole cells of N7XX-4T was anteiso-C15 : 0 (29.9 %).
32234114	8	7	from	differences	830:840	arg1	characteristics					870:884	phenotypic and genotypic characteristics	845:884	phenotypic and genotypic characteristics	845:884	On the basis of differences in phenotypic and genotypic characteristics, strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T) is designated as the type strain of a novel species of the genus Lysinimonas, for which the name Lysinimonas yzui sp.
32234114	4	8	theme	16S	524:526	arg1	MSL-13T					508:514	Lysinimonas kribbensis MSL-13T	485:514	Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity)	485:557	The nearest phylogenetic neighbour was Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity).
32234114	4	8	theme	16S	524:526	arg1	similarity					547:556	97.8 % 16S rRNA gene sequence similarity	517:556	97.8 % 16S rRNA gene sequence similarity	517:556	The nearest phylogenetic neighbour was Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity).
32234114	8	9	theme	genus	1002:1006	arg1	Lysinimonas					1008:1018	the genus Lysinimonas	998:1018	the genus Lysinimonas	998:1018	On the basis of differences in phenotypic and genotypic characteristics, strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T) is designated as the type strain of a novel species of the genus Lysinimonas, for which the name Lysinimonas yzui sp.
32234114	2	10	theme	yellow-pigmented	81:96	arg1	bacterium					163:171	A yellow-pigmented, Gram-stain-negative, aerobic, non-motile rod shaped, mesophilic bacterium	79:171	A yellow-pigmented, Gram-stain-negative, aerobic, non-motile rod shaped, mesophilic bacterium	79:171	A yellow-pigmented, Gram-stain-negative, aerobic, non-motile rod shaped, mesophilic bacterium, designated strain N7XX-4T, was isolated from cattail root grown on the mine tailings of Phoenix mountain, Tongling city, Anhui Province (PR China).
32234114	4	11	theme	sequence	538:545	arg1	MSL-13T					508:514	Lysinimonas kribbensis MSL-13T	485:514	Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity)	485:557	The nearest phylogenetic neighbour was Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity).
32234114	4	11	theme	sequence	538:545	arg1	similarity					547:556	97.8 % 16S rRNA gene sequence similarity	517:556	97.8 % 16S rRNA gene sequence similarity	517:556	The nearest phylogenetic neighbour was Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity).
32234114	7	12	with	B1δ	757:759	arg1	l-Lys					766:770	l-Lys	766:770	l-Lys as the diagnostic cell-wall diamino acid	766:811	The peptidoglycan type of the isolate was B1δ with l-Lys as the diagnostic cell-wall diamino acid.
32234114	7	13	theme	peptidoglycan	719:731	arg1	B1δ					757:759	B1δ	757:759	B1δ	757:759	The peptidoglycan type of the isolate was B1δ with l-Lys as the diagnostic cell-wall diamino acid.
32234114	7	13	theme	peptidoglycan	719:731	arg1	type					733:736	The peptidoglycan type	715:736	The peptidoglycan type of the isolate	715:751	The peptidoglycan type of the isolate was B1δ with l-Lys as the diagnostic cell-wall diamino acid.
32234114	1	14	attach	isolated	27:34	arg1	cattail					41:47	cattail	41:47	cattail	41:47	nov., isolated from cattail root soil from mine tailings.
32234114	1	14	attach	isolated	27:34	arg2	nov.					21:24	nov.	21:24	nov.	21:24	nov., isolated from cattail root soil from mine tailings.
32234114	8	15	theme	phenotypic	845:854	arg1	characteristics					870:884	phenotypic and genotypic characteristics	845:884	phenotypic and genotypic characteristics	845:884	On the basis of differences in phenotypic and genotypic characteristics, strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T) is designated as the type strain of a novel species of the genus Lysinimonas, for which the name Lysinimonas yzui sp.
32234114	3	16	theme	family	420:425	arg1	Microbacteriaceae					427:443	the family Microbacteriaceae	416:443	the family Microbacteriaceae	416:443	Analysis of the 16S rRNA gene sequence revealed that the strain represented a novel member of the family Microbacteriaceae.
32234114	8	17	theme	1.16548T=DSM	910:921	arg1	N7XX-4T					894:900	strain N7XX-4T	887:900	strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T)	887:941	On the basis of differences in phenotypic and genotypic characteristics, strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T) is designated as the type strain of a novel species of the genus Lysinimonas, for which the name Lysinimonas yzui sp.
32234114	8	17	theme	1.16548T=DSM	910:921	arg1	32630T					935:940	=CGMCC 1.16548T=DSM 106791T=JCM 32630T	903:940	=CGMCC 1.16548T=DSM 106791T=JCM 32630T	903:940	On the basis of differences in phenotypic and genotypic characteristics, strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T) is designated as the type strain of a novel species of the genus Lysinimonas, for which the name Lysinimonas yzui sp.
32234114	2	18	theme	mine	245:248	arg1	tailings					250:257	the mine tailings	241:257	the mine tailings of Phoenix mountain, Tongling city, Anhui Province (PR China)	241:319	A yellow-pigmented, Gram-stain-negative, aerobic, non-motile rod shaped, mesophilic bacterium, designated strain N7XX-4T, was isolated from cattail root grown on the mine tailings of Phoenix mountain, Tongling city, Anhui Province (PR China).
32234114	4	19	theme	gene	533:536	arg1	MSL-13T					508:514	Lysinimonas kribbensis MSL-13T	485:514	Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity)	485:557	The nearest phylogenetic neighbour was Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity).
32234114	4	19	theme	gene	533:536	arg1	similarity					547:556	97.8 % 16S rRNA gene sequence similarity	517:556	97.8 % 16S rRNA gene sequence similarity	517:556	The nearest phylogenetic neighbour was Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity).
32234114	4	20	theme	rRNA	528:531	arg1	MSL-13T					508:514	Lysinimonas kribbensis MSL-13T	485:514	Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity)	485:557	The nearest phylogenetic neighbour was Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity).
32234114	4	20	theme	rRNA	528:531	arg1	similarity					547:556	97.8 % 16S rRNA gene sequence similarity	517:556	97.8 % 16S rRNA gene sequence similarity	517:556	The nearest phylogenetic neighbour was Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity).
32234114	7	21	theme	cell-wall	790:798	arg1	acid					808:811	the diagnostic cell-wall diamino acid	775:811	the diagnostic cell-wall diamino acid	775:811	The peptidoglycan type of the isolate was B1δ with l-Lys as the diagnostic cell-wall diamino acid.
32234114	0	22	theme	yzui	12:15	arg1	sp					17:18	Lysinimonas yzui sp	0:18	Lysinimonas yzui sp.	0:19	Lysinimonas yzui sp.
32234114	5	23	theme	fatty	578:582	arg1	acid					584:587	The most abundant fatty acid	560:587	The most abundant fatty acid in whole cells of N7XX-4T	560:613	The most abundant fatty acid in whole cells of N7XX-4T was anteiso-C15 : 0 (29.9 %).
32234114	5	23	theme	fatty	578:582	arg1	 0					632:633	 0	632:633	 0	632:633	The most abundant fatty acid in whole cells of N7XX-4T was anteiso-C15 : 0 (29.9 %).
32234114	7	24	theme	diamino	800:806	arg1	acid					808:811	the diagnostic cell-wall diamino acid	775:811	the diagnostic cell-wall diamino acid	775:811	The peptidoglycan type of the isolate was B1δ with l-Lys as the diagnostic cell-wall diamino acid.
32234114	1	25	theme	mine	64:67	arg1	tailings					69:76	mine tailings	64:76	mine tailings	64:76	nov., isolated from cattail root soil from mine tailings.
32234114	0	26	theme	Lysinimonas	0:10	arg1	sp					17:18	Lysinimonas yzui sp	0:18	Lysinimonas yzui sp.	0:19	Lysinimonas yzui sp.
32234114	8	27	theme	106791T=JCM	923:933	arg1	N7XX-4T					894:900	strain N7XX-4T	887:900	strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T)	887:941	On the basis of differences in phenotypic and genotypic characteristics, strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T) is designated as the type strain of a novel species of the genus Lysinimonas, for which the name Lysinimonas yzui sp.
32234114	8	27	theme	106791T=JCM	923:933	arg1	32630T					935:940	=CGMCC 1.16548T=DSM 106791T=JCM 32630T	903:940	=CGMCC 1.16548T=DSM 106791T=JCM 32630T	903:940	On the basis of differences in phenotypic and genotypic characteristics, strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T) is designated as the type strain of a novel species of the genus Lysinimonas, for which the name Lysinimonas yzui sp.
32234114	8	28	theme	name	1035:1038	arg1	sp					1057:1058	the name Lysinimonas yzui sp	1031:1058	the name Lysinimonas yzui sp	1031:1058	On the basis of differences in phenotypic and genotypic characteristics, strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T) is designated as the type strain of a novel species of the genus Lysinimonas, for which the name Lysinimonas yzui sp.
32234114	5	29	from	acid	584:587	arg1	cells					598:602	whole cells	592:602	whole cells of N7XX-4T	592:613	The most abundant fatty acid in whole cells of N7XX-4T was anteiso-C15 : 0 (29.9 %).
32234114	7	30	theme	diagnostic	779:788	arg1	acid					808:811	the diagnostic cell-wall diamino acid	775:811	the diagnostic cell-wall diamino acid	775:811	The peptidoglycan type of the isolate was B1δ with l-Lys as the diagnostic cell-wall diamino acid.
32234114	8	31	theme	yzui	1052:1055	arg1	sp					1057:1058	the name Lysinimonas yzui sp	1031:1058	the name Lysinimonas yzui sp	1031:1058	On the basis of differences in phenotypic and genotypic characteristics, strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T) is designated as the type strain of a novel species of the genus Lysinimonas, for which the name Lysinimonas yzui sp.
32234114	8	32	dep	differences	830:840	arg1	basis					821:825	basis	821:825	basis	821:825	On the basis of differences in phenotypic and genotypic characteristics, strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T) is designated as the type strain of a novel species of the genus Lysinimonas, for which the name Lysinimonas yzui sp.
32234114	8	32	dep	differences	830:840	arg1	the					817:819	the	817:819	the	817:819	On the basis of differences in phenotypic and genotypic characteristics, strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T) is designated as the type strain of a novel species of the genus Lysinimonas, for which the name Lysinimonas yzui sp.
32234114	3	33	theme	novel	400:404	arg1	member					406:411	a novel member	398:411	a novel member of the family Microbacteriaceae	398:443	Analysis of the 16S rRNA gene sequence revealed that the strain represented a novel member of the family Microbacteriaceae.
32234114	4	34	theme	%	522:522	arg1	MSL-13T					508:514	Lysinimonas kribbensis MSL-13T	485:514	Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity)	485:557	The nearest phylogenetic neighbour was Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity).
32234114	4	34	theme	%	522:522	arg1	similarity					547:556	97.8 % 16S rRNA gene sequence similarity	517:556	97.8 % 16S rRNA gene sequence similarity	517:556	The nearest phylogenetic neighbour was Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity).
32234114	3	35	theme	gene	347:350	arg1	sequence					352:359	the 16S rRNA gene sequence	334:359	the 16S rRNA gene sequence	334:359	Analysis of the 16S rRNA gene sequence revealed that the strain represented a novel member of the family Microbacteriaceae.
32234114	4	36	theme	97.8 	517:521	arg1	%					522:522	%	522:522	%	522:522	The nearest phylogenetic neighbour was Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity).
32234114	3	37	theme	sequence	352:359	arg1	Analysis					322:329	Analysis	322:329	Analysis of the 16S rRNA gene sequence	322:359	Analysis of the 16S rRNA gene sequence revealed that the strain represented a novel member of the family Microbacteriaceae.
32234114	8	38	theme	Lysinimonas	1040:1050	arg1	sp					1057:1058	the name Lysinimonas yzui sp	1031:1058	the name Lysinimonas yzui sp	1031:1058	On the basis of differences in phenotypic and genotypic characteristics, strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T) is designated as the type strain of a novel species of the genus Lysinimonas, for which the name Lysinimonas yzui sp.
32234114	8	39	theme	genotypic	860:868	arg1	characteristics					870:884	phenotypic and genotypic characteristics	845:884	phenotypic and genotypic characteristics	845:884	On the basis of differences in phenotypic and genotypic characteristics, strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T) is designated as the type strain of a novel species of the genus Lysinimonas, for which the name Lysinimonas yzui sp.
32234114	8	40	theme	=CGMCC	903:908	arg1	N7XX-4T					894:900	strain N7XX-4T	887:900	strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T)	887:941	On the basis of differences in phenotypic and genotypic characteristics, strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T) is designated as the type strain of a novel species of the genus Lysinimonas, for which the name Lysinimonas yzui sp.
32234114	8	40	theme	=CGMCC	903:908	arg1	32630T					935:940	=CGMCC 1.16548T=DSM 106791T=JCM 32630T	903:940	=CGMCC 1.16548T=DSM 106791T=JCM 32630T	903:940	On the basis of differences in phenotypic and genotypic characteristics, strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T) is designated as the type strain of a novel species of the genus Lysinimonas, for which the name Lysinimonas yzui sp.
32234114	8	41	theme	Lysinimonas	1008:1018	arg1	species					987:993	a novel species	979:993	a novel species	979:993	On the basis of differences in phenotypic and genotypic characteristics, strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T) is designated as the type strain of a novel species of the genus Lysinimonas, for which the name Lysinimonas yzui sp.
32234114	3	42	theme	16S	338:340	arg1	rRNA					342:345	16S rRNA	338:345	the 16S rRNA gene sequence	334:359	Analysis of the 16S rRNA gene sequence revealed that the strain represented a novel member of the family Microbacteriaceae.
32234114	2	43	dep	yellow-pigmented	81:96	arg1	Gram-stain-negative					99:117	Gram-stain-negative	99:117	Gram-stain-negative	99:117	A yellow-pigmented, Gram-stain-negative, aerobic, non-motile rod shaped, mesophilic bacterium, designated strain N7XX-4T, was isolated from cattail root grown on the mine tailings of Phoenix mountain, Tongling city, Anhui Province (PR China).
32234114	2	43	dep	yellow-pigmented	81:96	arg1	mesophilic					152:161	mesophilic	152:161	mesophilic	152:161	A yellow-pigmented, Gram-stain-negative, aerobic, non-motile rod shaped, mesophilic bacterium, designated strain N7XX-4T, was isolated from cattail root grown on the mine tailings of Phoenix mountain, Tongling city, Anhui Province (PR China).
32234114	2	43	dep	yellow-pigmented	81:96	arg1	shaped					144:149	shaped	144:149	shaped	144:149	A yellow-pigmented, Gram-stain-negative, aerobic, non-motile rod shaped, mesophilic bacterium, designated strain N7XX-4T, was isolated from cattail root grown on the mine tailings of Phoenix mountain, Tongling city, Anhui Province (PR China).
32234114	2	43	dep	yellow-pigmented	81:96	arg1	aerobic					120:126	aerobic	120:126	aerobic	120:126	A yellow-pigmented, Gram-stain-negative, aerobic, non-motile rod shaped, mesophilic bacterium, designated strain N7XX-4T, was isolated from cattail root grown on the mine tailings of Phoenix mountain, Tongling city, Anhui Province (PR China).
32234114	5	44	theme	N7XX-4T	607:613	arg1	cells					598:602	whole cells	592:602	whole cells of N7XX-4T	592:613	The most abundant fatty acid in whole cells of N7XX-4T was anteiso-C15 : 0 (29.9 %).
32234114	2	45	theme	Province	301:308	arg1	tailings					250:257	the mine tailings	241:257	the mine tailings of Phoenix mountain, Tongling city, Anhui Province (PR China)	241:319	A yellow-pigmented, Gram-stain-negative, aerobic, non-motile rod shaped, mesophilic bacterium, designated strain N7XX-4T, was isolated from cattail root grown on the mine tailings of Phoenix mountain, Tongling city, Anhui Province (PR China).
32234114	3	46	theme	rRNA	342:345	arg1	sequence					352:359	the 16S rRNA gene sequence	334:359	the 16S rRNA gene sequence	334:359	Analysis of the 16S rRNA gene sequence revealed that the strain represented a novel member of the family Microbacteriaceae.
32234114	4	47	theme	kribbensis	497:506	arg1	neighbour					471:479	The nearest phylogenetic neighbour	446:479	The nearest phylogenetic neighbour	446:479	The nearest phylogenetic neighbour was Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity).
32234114	4	47	theme	kribbensis	497:506	arg1	similarity					547:556	97.8 % 16S rRNA gene sequence similarity	517:556	97.8 % 16S rRNA gene sequence similarity	517:556	The nearest phylogenetic neighbour was Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity).
32234114	4	47	theme	kribbensis	497:506	arg1	MSL-13T					508:514	Lysinimonas kribbensis MSL-13T	485:514	Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity)	485:557	The nearest phylogenetic neighbour was Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity).
32234114	5	48	theme	abundant	569:576	arg1	acid					584:587	The most abundant fatty acid	560:587	The most abundant fatty acid in whole cells of N7XX-4T	560:613	The most abundant fatty acid in whole cells of N7XX-4T was anteiso-C15 : 0 (29.9 %).
32234114	5	48	theme	abundant	569:576	arg1	 0					632:633	 0	632:633	 0	632:633	The most abundant fatty acid in whole cells of N7XX-4T was anteiso-C15 : 0 (29.9 %).
32234114	3	49	theme	Microbacteriaceae	427:443	arg1	member					406:411	a novel member	398:411	a novel member of the family Microbacteriaceae	398:443	Analysis of the 16S rRNA gene sequence revealed that the strain represented a novel member of the family Microbacteriaceae.
32234114	4	50	theme	Lysinimonas	485:495	arg1	neighbour					471:479	The nearest phylogenetic neighbour	446:479	The nearest phylogenetic neighbour	446:479	The nearest phylogenetic neighbour was Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity).
32234114	4	50	theme	Lysinimonas	485:495	arg1	similarity					547:556	97.8 % 16S rRNA gene sequence similarity	517:556	97.8 % 16S rRNA gene sequence similarity	517:556	The nearest phylogenetic neighbour was Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity).
32234114	4	50	theme	Lysinimonas	485:495	arg1	MSL-13T					508:514	Lysinimonas kribbensis MSL-13T	485:514	Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity)	485:557	The nearest phylogenetic neighbour was Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity).
32234114	2	51	theme	strain	185:190	arg1	N7XX-4T					192:198	strain N7XX-4T	185:198	strain N7XX-4T	185:198	A yellow-pigmented, Gram-stain-negative, aerobic, non-motile rod shaped, mesophilic bacterium, designated strain N7XX-4T, was isolated from cattail root grown on the mine tailings of Phoenix mountain, Tongling city, Anhui Province (PR China).
32234114	4	52	theme	nearest	450:456	arg1	neighbour					471:479	The nearest phylogenetic neighbour	446:479	The nearest phylogenetic neighbour	446:479	The nearest phylogenetic neighbour was Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity).
32234114	4	52	theme	nearest	450:456	arg1	MSL-13T					508:514	Lysinimonas kribbensis MSL-13T	485:514	Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity)	485:557	The nearest phylogenetic neighbour was Lysinimonas kribbensis MSL-13T (97.8 % 16S rRNA gene sequence similarity).
32234114	6	53	theme	predominant	649:659	arg1	MK-12					679:683	MK-12	679:683	MK-12	679:683	The predominant menaquinones were MK-12(H2), MK-13(H2) and MK-11(H2).
32234114	6	53	theme	predominant	649:659	arg1	menaquinones					661:672	The predominant menaquinones	645:672	The predominant menaquinones	645:672	The predominant menaquinones were MK-12(H2), MK-13(H2) and MK-11(H2).
32234114	8	54	theme	novel	981:985	arg1	species					987:993	a novel species	979:993	a novel species	979:993	On the basis of differences in phenotypic and genotypic characteristics, strain N7XX-4T (=CGMCC 1.16548T=DSM 106791T=JCM 32630T) is designated as the type strain of a novel species of the genus Lysinimonas, for which the name Lysinimonas yzui sp.
32234114	2	55	theme	cattail	219:225	arg1	root					227:230	cattail root	219:230	cattail root grown on the mine tailings of Phoenix mountain, Tongling city, Anhui Province (PR China)	219:319	A yellow-pigmented, Gram-stain-negative, aerobic, non-motile rod shaped, mesophilic bacterium, designated strain N7XX-4T, was isolated from cattail root grown on the mine tailings of Phoenix mountain, Tongling city, Anhui Province (PR China).
32234114	7	56	theme	isolate	745:751	arg1	B1δ					757:759	B1δ	757:759	B1δ	757:759	The peptidoglycan type of the isolate was B1δ with l-Lys as the diagnostic cell-wall diamino acid.
32234114	7	56	theme	isolate	745:751	arg1	type					733:736	The peptidoglycan type	715:736	The peptidoglycan type of the isolate	715:751	The peptidoglycan type of the isolate was B1δ with l-Lys as the diagnostic cell-wall diamino acid.
35141446	4	0	theme	eCG	827:829	arg1	proteins					841:848	recombinant eCG (rec-eCG) proteins	815:848	recombinant eCG (rec-eCG) proteins	815:848	We produced recombinant eCG (rec-eCG) proteins in Chinese hamster ovary suspension (CHO-S) cells.
35141446	1	1	theme	-like	340:344	arg1	effects					390:396	dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects	311:396	dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects	311:396	Equine chorionic gonadotropin (eCG), produced by the endometrial cups of the placenta after the first trimester, is a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species.
35141446	9	2	gly	glycosylation	1586:1598	arg2	sites					1600:1604	specific glycosylation sites	1577:1604	specific glycosylation sites	1577:1604	These results provide important information on rec-eCG in the regulation of specific glycosylation sites and improve our understanding of the specific biological activity of rec-eCG glycosylation sites in equidaes.
35141446	1	3	theme	placenta	234:241	arg1	cups					222:225	the endometrial cups	206:225	the endometrial cups of the placenta	206:241	Equine chorionic gonadotropin (eCG), produced by the endometrial cups of the placenta after the first trimester, is a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species.
35141446	4	4	theme	rec-eCG	832:838	arg1	proteins					841:848	recombinant eCG (rec-eCG) proteins	815:848	recombinant eCG (rec-eCG) proteins	815:848	We produced recombinant eCG (rec-eCG) proteins in Chinese hamster ovary suspension (CHO-S) cells.
35141446	8	5	link	O-linked	1443:1450	arg1	sites					1466:1470	O-linked glycosylation sites	1443:1470	O-linked glycosylation sites in cells expressing eLH/CGR	1443:1498	Our results indicate that the biological activity of eCG is greatly affected by the removal of N- and O-linked glycosylation sites in cells expressing eLH/CGR.
35141446	3	6	theme	site	692:695	arg1	substitution					641:652	substitution	641:652	substitution of α-subunit56 N-linked glycosylation site	641:695	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	6	theme	site	692:695	arg1	eCGβ/αΔ56					630:638	eCGβ/αΔ56	630:638	eCGβ/αΔ56	630:638	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	7	theme	biological	503:512	arg1	functions					514:522	the specific biological functions	490:522	the specific biological functions of glycosylated sites in eCG	490:551	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	8	theme	N-linked	669:676	arg1	site					692:695	α-subunit56 N-linked glycosylation site	657:695	α-subunit56 N-linked glycosylation site	657:695	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	9	9	theme	specific	1577:1584	arg1	sites					1600:1604	specific glycosylation sites	1577:1604	specific glycosylation sites	1577:1604	These results provide important information on rec-eCG in the regulation of specific glycosylation sites and improve our understanding of the specific biological activity of rec-eCG glycosylation sites in equidaes.
35141446	7	10	theme	eCG	1313:1315	arg1	eCG					1313:1315	wild-type eCG	1303:1315	wild-type eCG (141.9 nmol/104 cells)	1303:1338	The Rmax values of the mutants were 56%-80% those of wild-type eCG (141.9 nmol/104 cells).
35141446	7	10	theme	eCG	1313:1315	arg1	values					1259:1264	The Rmax values	1250:1264	The Rmax values of the mutants	1250:1279	The Rmax values of the mutants were 56%-80% those of wild-type eCG (141.9 nmol/104 cells).
35141446	7	10	theme	eCG	1313:1315	arg1	cells					1333:1337	141.9 nmol/104 cells	1318:1337	141.9 nmol/104 cells	1318:1337	The Rmax values of the mutants were 56%-80% those of wild-type eCG (141.9 nmol/104 cells).
35141446	7	10	theme	eCG	1313:1315	arg1	%					1292:1292	56%-80%	1286:1292	56%-80% those of wild-type eCG (141.9 nmol/104 cells)	1286:1338	The Rmax values of the mutants were 56%-80% those of wild-type eCG (141.9 nmol/104 cells).
35141446	3	11	theme	sites	540:544	arg1	functions					514:522	the specific biological functions	490:522	the specific biological functions of glycosylated sites in eCG	490:551	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	4	12	theme	ovary	869:873	arg1	cells					894:898	Chinese hamster ovary suspension (CHO-S) cells	853:898	Chinese hamster ovary suspension (CHO-S) cells	853:898	We produced recombinant eCG (rec-eCG) proteins in Chinese hamster ovary suspension (CHO-S) cells.
35141446	0	13	theme	Luteinizing	115:125	arg1	Receptor					147:154	Equine Luteinizing Hormone/CG (eLH/CG) Receptor	108:154	Equine Luteinizing Hormone/CG (eLH/CG) Receptor	108:154	Specific Biological Activity of Equine Chorionic Gonadotropin (eCG) Glycosylation Sites in Cells Expressing Equine Luteinizing Hormone/CG (eLH/CG) Receptor.
35141446	5	14	gly	deglycosylated	1053:1066	arg1	mutants					1068:1074	deglycosylated mutants	1053:1074	deglycosylated mutants	1053:1074	We examined the biological activity of rec-eCG proteins in CHO-K1 cells expressing the eLH/CG receptor and found that signal transduction activities of deglycosylated mutants remarkably decreased.
35141446	3	15	theme	glycosylation	733:745	arg1	sites					747:751	the O-linked glycosylation sites	720:751	the O-linked glycosylation sites at the β-subunit	720:768	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	5	16	theme	signal	1019:1024	arg1	activities					1039:1048	signal transduction activities	1019:1048	signal transduction activities of deglycosylated mutants	1019:1074	We examined the biological activity of rec-eCG proteins in CHO-K1 cells expressing the eLH/CG receptor and found that signal transduction activities of deglycosylated mutants remarkably decreased.
35141446	7	17	theme	%	1288:1288	arg1	eCG					1313:1315	wild-type eCG	1303:1315	wild-type eCG (141.9 nmol/104 cells)	1303:1338	The Rmax values of the mutants were 56%-80% those of wild-type eCG (141.9 nmol/104 cells).
35141446	7	17	theme	%	1288:1288	arg1	values					1259:1264	The Rmax values	1250:1264	The Rmax values of the mutants	1250:1279	The Rmax values of the mutants were 56%-80% those of wild-type eCG (141.9 nmol/104 cells).
35141446	7	17	theme	%	1288:1288	arg1	cells					1333:1337	141.9 nmol/104 cells	1318:1337	141.9 nmol/104 cells	1318:1337	The Rmax values of the mutants were 56%-80% those of wild-type eCG (141.9 nmol/104 cells).
35141446	7	17	theme	%	1288:1288	arg1	%					1292:1292	56%-80%	1286:1292	56%-80% those of wild-type eCG (141.9 nmol/104 cells)	1286:1338	The Rmax values of the mutants were 56%-80% those of wild-type eCG (141.9 nmol/104 cells).
35141446	3	18	link	O-linked	724:731	arg1	sites					747:751	the O-linked glycosylation sites	720:751	the O-linked glycosylation sites at the β-subunit	720:768	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	9	19	from	rec-eCG	1548:1554	arg1	regulation					1563:1572	the regulation	1559:1572	the regulation of specific glycosylation sites	1559:1604	These results provide important information on rec-eCG in the regulation of specific glycosylation sites and improve our understanding of the specific biological activity of rec-eCG glycosylation sites in equidaes.
35141446	4	20	theme	Chinese	853:859	arg1	cells					894:898	Chinese hamster ovary suspension (CHO-S) cells	853:898	Chinese hamster ovary suspension (CHO-S) cells	853:898	We produced recombinant eCG (rec-eCG) proteins in Chinese hamster ovary suspension (CHO-S) cells.
35141446	1	21	theme	-like	384:388	arg1	effects					390:396	dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects	311:396	dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects	311:396	Equine chorionic gonadotropin (eCG), produced by the endometrial cups of the placenta after the first trimester, is a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species.
35141446	3	22	theme	site	583:586	arg1	deletion					708:715	deletion	708:715	deletion of the O-linked glycosylation sites at the β-subunit	708:768	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	22	theme	site	583:586	arg1	eCGβ-D/αΔ56					775:785	eCGβ-D/αΔ56	775:785	eCGβ-D/αΔ56	775:785	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	22	theme	site	583:586	arg1	mutants					588:594	the following site mutants	569:594	the following site mutants of N- and O-linked glycosylation	569:627	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	22	theme	site	583:586	arg1	mutant					795:800	double mutant	788:800	double mutant	788:800	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	22	theme	site	583:586	arg1	eCGβ-D/α					698:705	eCGβ-D/α	698:705	eCGβ-D/α	698:705	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	9	23	theme	biological	1652:1661	arg1	activity					1663:1670	the specific biological activity	1639:1670	the specific biological activity of rec-eCG glycosylation sites in equidaes	1639:1713	These results provide important information on rec-eCG in the regulation of specific glycosylation sites and improve our understanding of the specific biological activity of rec-eCG glycosylation sites in equidaes.
35141446	8	24	theme	biological	1371:1380	arg1	activity					1382:1389	the biological activity	1367:1389	the biological activity of eCG	1367:1396	Our results indicate that the biological activity of eCG is greatly affected by the removal of N- and O-linked glycosylation sites in cells expressing eLH/CGR.
35141446	6	25	theme	mutants	1154:1160	arg1	levels					1107:1112	The EC50 levels	1098:1112	The EC50 levels of eCGβ/αΔ56, eCGβ-D/α, and eCGβ-D/αΔ56 mutants	1098:1160	The EC50 levels of eCGβ/αΔ56, eCGβ-D/α, and eCGβ-D/αΔ56 mutants decreased by 2.1-, 5.6-, and 3.4-fold, respectively, compared to that of wild-type eCG.
35141446	0	26	from	Activity	20:27	arg1	Cells					91:95	Cells	91:95	Cells Expressing Equine Luteinizing Hormone/CG (eLH/CG) Receptor	91:154	Specific Biological Activity of Equine Chorionic Gonadotropin (eCG) Glycosylation Sites in Cells Expressing Equine Luteinizing Hormone/CG (eLH/CG) Receptor.
35141446	0	27	theme	Glycosylation	68:80	arg1	Sites					82:86	Equine Chorionic Gonadotropin (eCG) Glycosylation Sites	32:86	Equine Chorionic Gonadotropin (eCG) Glycosylation Sites	32:86	Specific Biological Activity of Equine Chorionic Gonadotropin (eCG) Glycosylation Sites in Cells Expressing Equine Luteinizing Hormone/CG (eLH/CG) Receptor.
35141446	1	28	theme	luteinizing	316:326	arg1	LH					337:338	LH	337:338	LH	337:338	Equine chorionic gonadotropin (eCG), produced by the endometrial cups of the placenta after the first trimester, is a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species.
35141446	1	28	theme	luteinizing	316:326	arg1	hormone					328:334	luteinizing hormone	316:334	luteinizing hormone (LH)	316:339	Equine chorionic gonadotropin (eCG), produced by the endometrial cups of the placenta after the first trimester, is a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species.
35141446	1	29	theme	Equine	157:162	arg1	eCG					188:190	eCG	188:190	eCG	188:190	Equine chorionic gonadotropin (eCG), produced by the endometrial cups of the placenta after the first trimester, is a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species.
35141446	1	29	theme	Equine	157:162	arg1	glycoprotein					284:295	a specific glycoprotein	273:295	a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species	273:417	Equine chorionic gonadotropin (eCG), produced by the endometrial cups of the placenta after the first trimester, is a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species.
35141446	1	29	theme	Equine	157:162	arg1	gonadotropin					174:185	Equine chorionic gonadotropin	157:185	Equine chorionic gonadotropin (eCG)	157:191	Equine chorionic gonadotropin (eCG), produced by the endometrial cups of the placenta after the first trimester, is a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species.
35141446	9	30	theme	glycosylation	1683:1695	arg1	sites					1697:1701	rec-eCG glycosylation sites	1675:1701	rec-eCG glycosylation sites in equidaes	1675:1713	These results provide important information on rec-eCG in the regulation of specific glycosylation sites and improve our understanding of the specific biological activity of rec-eCG glycosylation sites in equidaes.
35141446	3	31	from	eCGβ-D/α	698:705	arg1	β-subunit					760:768	the β-subunit	756:768	the β-subunit	756:768	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	5	32	theme	deglycosylated	1053:1066	arg1	mutants					1068:1074	deglycosylated mutants	1053:1074	deglycosylated mutants	1053:1074	We examined the biological activity of rec-eCG proteins in CHO-K1 cells expressing the eLH/CG receptor and found that signal transduction activities of deglycosylated mutants remarkably decreased.
35141446	8	33	from	removal	1425:1431	arg1	cells					1475:1479	cells	1475:1479	cells expressing eLH/CGR	1475:1498	Our results indicate that the biological activity of eCG is greatly affected by the removal of N- and O-linked glycosylation sites in cells expressing eLH/CGR.
35141446	5	34	theme	biological	917:926	arg1	activity					928:935	the biological activity	913:935	the biological activity of rec-eCG proteins	913:955	We examined the biological activity of rec-eCG proteins in CHO-K1 cells expressing the eLH/CG receptor and found that signal transduction activities of deglycosylated mutants remarkably decreased.
35141446	3	35	from	deletion	708:715	arg1	β-subunit					760:768	the β-subunit	756:768	the β-subunit	756:768	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	0	36	theme	Specific	0:7	arg1	Activity					20:27	Specific Biological Activity	0:27	Specific Biological Activity of Equine Chorionic Gonadotropin (eCG) Glycosylation Sites in Cells Expressing Equine Luteinizing Hormone/CG (eLH/CG) Receptor	0:154	Specific Biological Activity of Equine Chorionic Gonadotropin (eCG) Glycosylation Sites in Cells Expressing Equine Luteinizing Hormone/CG (eLH/CG) Receptor.
35141446	1	37	theme	first	253:257	arg1	trimester					259:267	the first trimester	249:267	the first trimester	249:267	Equine chorionic gonadotropin (eCG), produced by the endometrial cups of the placenta after the first trimester, is a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species.
35141446	7	38	theme	Rmax	1254:1257	arg1	eCG					1313:1315	wild-type eCG	1303:1315	wild-type eCG (141.9 nmol/104 cells)	1303:1338	The Rmax values of the mutants were 56%-80% those of wild-type eCG (141.9 nmol/104 cells).
35141446	7	38	theme	Rmax	1254:1257	arg1	%					1292:1292	56%-80%	1286:1292	56%-80% those of wild-type eCG (141.9 nmol/104 cells)	1286:1338	The Rmax values of the mutants were 56%-80% those of wild-type eCG (141.9 nmol/104 cells).
35141446	7	38	theme	Rmax	1254:1257	arg1	values					1259:1264	The Rmax values	1250:1264	The Rmax values of the mutants	1250:1279	The Rmax values of the mutants were 56%-80% those of wild-type eCG (141.9 nmol/104 cells).
35141446	0	39	theme	Equine	32:37	arg1	eCG					63:65	eCG	63:65	eCG	63:65	Specific Biological Activity of Equine Chorionic Gonadotropin (eCG) Glycosylation Sites in Cells Expressing Equine Luteinizing Hormone/CG (eLH/CG) Receptor.
35141446	0	39	theme	Equine	32:37	arg1	Gonadotropin					49:60	Equine Chorionic Gonadotropin	32:60	Equine Chorionic Gonadotropin (eCG) Glycosylation Sites	32:86	Specific Biological Activity of Equine Chorionic Gonadotropin (eCG) Glycosylation Sites in Cells Expressing Equine Luteinizing Hormone/CG (eLH/CG) Receptor.
35141446	5	40	theme	CHO-K1	960:965	arg1	cells					967:971	CHO-K1 cells	960:971	CHO-K1 cells expressing the eLH/CG receptor	960:1002	We examined the biological activity of rec-eCG proteins in CHO-K1 cells expressing the eLH/CG receptor and found that signal transduction activities of deglycosylated mutants remarkably decreased.
35141446	8	41	theme	glycosylation	1452:1464	arg1	sites					1466:1470	O-linked glycosylation sites	1443:1470	O-linked glycosylation sites in cells expressing eLH/CGR	1443:1498	Our results indicate that the biological activity of eCG is greatly affected by the removal of N- and O-linked glycosylation sites in cells expressing eLH/CGR.
35141446	0	42	theme	Gonadotropin	49:60	arg1	Sites					82:86	Equine Chorionic Gonadotropin (eCG) Glycosylation Sites	32:86	Equine Chorionic Gonadotropin (eCG) Glycosylation Sites	32:86	Specific Biological Activity of Equine Chorionic Gonadotropin (eCG) Glycosylation Sites in Cells Expressing Equine Luteinizing Hormone/CG (eLH/CG) Receptor.
35141446	3	43	link	O-linked	606:613	arg1	glycosylation					615:627	O-linked glycosylation	606:627	O-linked glycosylation	606:627	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	44	from	β-subunit	760:768	arg1	deletion					708:715	deletion	708:715	deletion of the O-linked glycosylation sites at the β-subunit	708:768	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	44	from	β-subunit	760:768	arg1	eCGβ-D/αΔ56					775:785	eCGβ-D/αΔ56	775:785	eCGβ-D/αΔ56	775:785	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	44	from	β-subunit	760:768	arg1	eCGβ-D/α					698:705	eCGβ-D/α	698:705	eCGβ-D/α	698:705	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	44	from	β-subunit	760:768	arg1	mutants					588:594	the following site mutants	569:594	the following site mutants of N- and O-linked glycosylation	569:627	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	44	from	β-subunit	760:768	arg1	sites					747:751	the O-linked glycosylation sites	720:751	the O-linked glycosylation sites at the β-subunit	720:768	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	45	dep	mutants	588:594	arg1	mutants					588:594	the following site mutants	569:594	the following site mutants of N- and O-linked glycosylation	569:627	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	45	dep	mutants	588:594	arg1	substitution					641:652	substitution	641:652	substitution of α-subunit56 N-linked glycosylation site	641:695	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	45	dep	mutants	588:594	arg1	eCGβ-D/αΔ56					775:785	eCGβ-D/αΔ56	775:785	eCGβ-D/αΔ56	775:785	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	45	dep	mutants	588:594	arg1	eCGβ-D/α					698:705	eCGβ-D/α	698:705	eCGβ-D/α	698:705	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	45	dep	mutants	588:594	arg1	deletion					708:715	deletion	708:715	deletion of the O-linked glycosylation sites at the β-subunit	708:768	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	45	dep	mutants	588:594	arg1	eCGβ/αΔ56					630:638	eCGβ/αΔ56	630:638	eCGβ/αΔ56	630:638	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	46	theme	glycosylation	615:627	arg1	deletion					708:715	deletion	708:715	deletion of the O-linked glycosylation sites at the β-subunit	708:768	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	46	theme	glycosylation	615:627	arg1	eCGβ-D/αΔ56					775:785	eCGβ-D/αΔ56	775:785	eCGβ-D/αΔ56	775:785	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	46	theme	glycosylation	615:627	arg1	mutants					588:594	the following site mutants	569:594	the following site mutants of N- and O-linked glycosylation	569:627	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	46	theme	glycosylation	615:627	arg1	mutant					795:800	double mutant	788:800	double mutant	788:800	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	46	theme	glycosylation	615:627	arg1	eCGβ-D/α					698:705	eCGβ-D/α	698:705	eCGβ-D/α	698:705	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	5	47	theme	proteins	948:955	arg1	activity					928:935	the biological activity	913:935	the biological activity of rec-eCG proteins	913:955	We examined the biological activity of rec-eCG proteins in CHO-K1 cells expressing the eLH/CG receptor and found that signal transduction activities of deglycosylated mutants remarkably decreased.
35141446	3	48	gly	glycosylation	733:745	arg2	sites					747:751	the O-linked glycosylation sites	720:751	the O-linked glycosylation sites at the β-subunit	720:768	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	48	gly	glycosylation	733:745	arg2	β-subunit					760:768	the β-subunit	756:768	the β-subunit	756:768	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	4	49	theme	recombinant	815:825	arg1	proteins					841:848	recombinant eCG (rec-eCG) proteins	815:848	recombinant eCG (rec-eCG) proteins	815:848	We produced recombinant eCG (rec-eCG) proteins in Chinese hamster ovary suspension (CHO-S) cells.
35141446	8	50	from	N-	1436:1437	arg1	cells					1475:1479	cells	1475:1479	cells expressing eLH/CGR	1475:1498	Our results indicate that the biological activity of eCG is greatly affected by the removal of N- and O-linked glycosylation sites in cells expressing eLH/CGR.
35141446	1	51	theme	non-equid	401:409	arg1	species					411:417	non-equid species	401:417	non-equid species	401:417	Equine chorionic gonadotropin (eCG), produced by the endometrial cups of the placenta after the first trimester, is a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species.
35141446	3	52	theme	specific	494:501	arg1	functions					514:522	the specific biological functions	490:522	the specific biological functions of glycosylated sites in eCG	490:551	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	53	link	N-linked	669:676	arg1	site					692:695	α-subunit56 N-linked glycosylation site	657:695	α-subunit56 N-linked glycosylation site	657:695	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	8	54	from	cells	1475:1479	arg1	removal					1425:1431	the removal	1421:1431	the removal of N- and O-linked glycosylation sites in cells expressing eLH/CGR	1421:1498	Our results indicate that the biological activity of eCG is greatly affected by the removal of N- and O-linked glycosylation sites in cells expressing eLH/CGR.
35141446	1	55	theme	chorionic	164:172	arg1	eCG					188:190	eCG	188:190	eCG	188:190	Equine chorionic gonadotropin (eCG), produced by the endometrial cups of the placenta after the first trimester, is a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species.
35141446	1	55	theme	chorionic	164:172	arg1	glycoprotein					284:295	a specific glycoprotein	273:295	a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species	273:417	Equine chorionic gonadotropin (eCG), produced by the endometrial cups of the placenta after the first trimester, is a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species.
35141446	1	55	theme	chorionic	164:172	arg1	gonadotropin					174:185	Equine chorionic gonadotropin	157:185	Equine chorionic gonadotropin (eCG)	157:191	Equine chorionic gonadotropin (eCG), produced by the endometrial cups of the placenta after the first trimester, is a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species.
35141446	7	56	theme	nmol/104	1324:1331	arg1	eCG					1313:1315	wild-type eCG	1303:1315	wild-type eCG (141.9 nmol/104 cells)	1303:1338	The Rmax values of the mutants were 56%-80% those of wild-type eCG (141.9 nmol/104 cells).
35141446	7	56	theme	nmol/104	1324:1331	arg1	cells					1333:1337	141.9 nmol/104 cells	1318:1337	141.9 nmol/104 cells	1318:1337	The Rmax values of the mutants were 56%-80% those of wild-type eCG (141.9 nmol/104 cells).
35141446	3	57	theme	glycosylation	678:690	arg1	site					692:695	α-subunit56 N-linked glycosylation site	657:695	α-subunit56 N-linked glycosylation site	657:695	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	8	58	from	sites	1466:1470	arg1	cells					1475:1479	cells	1475:1479	cells expressing eLH/CGR	1475:1498	Our results indicate that the biological activity of eCG is greatly affected by the removal of N- and O-linked glycosylation sites in cells expressing eLH/CGR.
35141446	3	59	theme	glycosylated	527:538	arg1	sites					540:544	glycosylated sites	527:544	glycosylated sites	527:544	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	7	60	dep	%	1292:1292	arg1	those					1294:1298	those	1294:1298	those	1294:1298	The Rmax values of the mutants were 56%-80% those of wild-type eCG (141.9 nmol/104 cells).
35141446	9	61	theme	glycosylation	1586:1598	arg1	sites					1600:1604	specific glycosylation sites	1577:1604	specific glycosylation sites	1577:1604	These results provide important information on rec-eCG in the regulation of specific glycosylation sites and improve our understanding of the specific biological activity of rec-eCG glycosylation sites in equidaes.
35141446	0	62	theme	Hormone/CG	127:136	arg1	Receptor					147:154	Equine Luteinizing Hormone/CG (eLH/CG) Receptor	108:154	Equine Luteinizing Hormone/CG (eLH/CG) Receptor	108:154	Specific Biological Activity of Equine Chorionic Gonadotropin (eCG) Glycosylation Sites in Cells Expressing Equine Luteinizing Hormone/CG (eLH/CG) Receptor.
35141446	4	63	theme	hamster	861:867	arg1	cells					894:898	Chinese hamster ovary suspension (CHO-S) cells	853:898	Chinese hamster ovary suspension (CHO-S) cells	853:898	We produced recombinant eCG (rec-eCG) proteins in Chinese hamster ovary suspension (CHO-S) cells.
35141446	6	64	theme	EC50	1102:1105	arg1	levels					1107:1112	The EC50 levels	1098:1112	The EC50 levels of eCGβ/αΔ56, eCGβ-D/α, and eCGβ-D/αΔ56 mutants	1098:1160	The EC50 levels of eCGβ/αΔ56, eCGβ-D/α, and eCGβ-D/αΔ56 mutants decreased by 2.1-, 5.6-, and 3.4-fold, respectively, compared to that of wild-type eCG.
35141446	8	65	theme	O-linked	1443:1450	arg1	sites					1466:1470	O-linked glycosylation sites	1443:1470	O-linked glycosylation sites in cells expressing eLH/CGR	1443:1498	Our results indicate that the biological activity of eCG is greatly affected by the removal of N- and O-linked glycosylation sites in cells expressing eLH/CGR.
35141446	0	66	theme	eLH/CG	139:144	arg1	Receptor					147:154	Equine Luteinizing Hormone/CG (eLH/CG) Receptor	108:154	Equine Luteinizing Hormone/CG (eLH/CG) Receptor	108:154	Specific Biological Activity of Equine Chorionic Gonadotropin (eCG) Glycosylation Sites in Cells Expressing Equine Luteinizing Hormone/CG (eLH/CG) Receptor.
35141446	1	67	theme	follicle-stimulating	350:369	arg1	hormone					371:377	follicle-stimulating hormone	350:377	follicle-stimulating hormone (FSH)	350:383	Equine chorionic gonadotropin (eCG), produced by the endometrial cups of the placenta after the first trimester, is a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species.
35141446	1	67	theme	follicle-stimulating	350:369	arg1	FSH					380:382	FSH	380:382	FSH	380:382	Equine chorionic gonadotropin (eCG), produced by the endometrial cups of the placenta after the first trimester, is a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species.
35141446	4	68	theme	suspension	875:884	arg1	cells					894:898	Chinese hamster ovary suspension (CHO-S) cells	853:898	Chinese hamster ovary suspension (CHO-S) cells	853:898	We produced recombinant eCG (rec-eCG) proteins in Chinese hamster ovary suspension (CHO-S) cells.
35141446	3	69	theme	sites	747:751	arg1	deletion					708:715	deletion	708:715	deletion of the O-linked glycosylation sites at the β-subunit	708:768	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	69	theme	sites	747:751	arg1	eCGβ-D/αΔ56					775:785	eCGβ-D/αΔ56	775:785	eCGβ-D/αΔ56	775:785	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	69	theme	sites	747:751	arg1	eCGβ-D/α					698:705	eCGβ-D/α	698:705	eCGβ-D/α	698:705	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	69	theme	sites	747:751	arg1	mutants					588:594	the following site mutants	569:594	the following site mutants of N- and O-linked glycosylation	569:627	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	5	70	theme	transduction	1026:1037	arg1	activities					1039:1048	signal transduction activities	1019:1048	signal transduction activities of deglycosylated mutants	1019:1074	We examined the biological activity of rec-eCG proteins in CHO-K1 cells expressing the eLH/CG receptor and found that signal transduction activities of deglycosylated mutants remarkably decreased.
35141446	9	71	theme	sites	1600:1604	arg1	regulation					1563:1572	the regulation	1559:1572	the regulation of specific glycosylation sites	1559:1604	These results provide important information on rec-eCG in the regulation of specific glycosylation sites and improve our understanding of the specific biological activity of rec-eCG glycosylation sites in equidaes.
35141446	3	72	gly	glycosylated	527:538	arg1	sites					540:544	glycosylated sites	527:544	glycosylated sites	527:544	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	73	from	functions	514:522	arg1	eCG					549:551	eCG	549:551	eCG	549:551	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	74	theme	O-linked	724:731	arg1	sites					747:751	the O-linked glycosylation sites	720:751	the O-linked glycosylation sites at the β-subunit	720:768	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	6	75	theme	wild-type	1235:1243	arg1	eCG					1245:1247	wild-type eCG	1235:1247	wild-type eCG	1235:1247	The EC50 levels of eCGβ/αΔ56, eCGβ-D/α, and eCGβ-D/αΔ56 mutants decreased by 2.1-, 5.6-, and 3.4-fold, respectively, compared to that of wild-type eCG.
35141446	5	76	theme	eLH/CG	988:993	arg1	receptor					995:1002	the eLH/CG receptor	984:1002	the eLH/CG receptor	984:1002	We examined the biological activity of rec-eCG proteins in CHO-K1 cells expressing the eLH/CG receptor and found that signal transduction activities of deglycosylated mutants remarkably decreased.
35141446	3	77	theme	following	573:581	arg1	deletion					708:715	deletion	708:715	deletion of the O-linked glycosylation sites at the β-subunit	708:768	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	77	theme	following	573:581	arg1	eCGβ-D/αΔ56					775:785	eCGβ-D/αΔ56	775:785	eCGβ-D/αΔ56	775:785	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	77	theme	following	573:581	arg1	mutants					588:594	the following site mutants	569:594	the following site mutants of N- and O-linked glycosylation	569:627	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	77	theme	following	573:581	arg1	mutant					795:800	double mutant	788:800	double mutant	788:800	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	77	theme	following	573:581	arg1	eCGβ-D/α					698:705	eCGβ-D/α	698:705	eCGβ-D/α	698:705	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	9	78	theme	specific	1643:1650	arg1	activity					1663:1670	the specific biological activity	1639:1670	the specific biological activity of rec-eCG glycosylation sites in equidaes	1639:1713	These results provide important information on rec-eCG in the regulation of specific glycosylation sites and improve our understanding of the specific biological activity of rec-eCG glycosylation sites in equidaes.
35141446	9	79	gly	glycosylation	1683:1695	arg2	sites					1697:1701	rec-eCG glycosylation sites	1675:1701	rec-eCG glycosylation sites in equidaes	1675:1713	These results provide important information on rec-eCG in the regulation of specific glycosylation sites and improve our understanding of the specific biological activity of rec-eCG glycosylation sites in equidaes.
35141446	9	80	theme	activity	1663:1670	arg1	understanding					1622:1634	our understanding	1618:1634	our understanding of the specific biological activity of rec-eCG glycosylation sites in equidaes	1618:1713	These results provide important information on rec-eCG in the regulation of specific glycosylation sites and improve our understanding of the specific biological activity of rec-eCG glycosylation sites in equidaes.
35141446	8	81	theme	eCG	1394:1396	arg1	activity					1382:1389	the biological activity	1367:1389	the biological activity of eCG	1367:1396	Our results indicate that the biological activity of eCG is greatly affected by the removal of N- and O-linked glycosylation sites in cells expressing eLH/CGR.
35141446	7	82	theme	mutants	1273:1279	arg1	eCG					1313:1315	wild-type eCG	1303:1315	wild-type eCG (141.9 nmol/104 cells)	1303:1338	The Rmax values of the mutants were 56%-80% those of wild-type eCG (141.9 nmol/104 cells).
35141446	7	82	theme	mutants	1273:1279	arg1	%					1292:1292	56%-80%	1286:1292	56%-80% those of wild-type eCG (141.9 nmol/104 cells)	1286:1338	The Rmax values of the mutants were 56%-80% those of wild-type eCG (141.9 nmol/104 cells).
35141446	7	82	theme	mutants	1273:1279	arg1	values					1259:1264	The Rmax values	1250:1264	The Rmax values of the mutants	1250:1279	The Rmax values of the mutants were 56%-80% those of wild-type eCG (141.9 nmol/104 cells).
35141446	9	83	theme	sites	1697:1701	arg1	activity					1663:1670	the specific biological activity	1639:1670	the specific biological activity of rec-eCG glycosylation sites in equidaes	1639:1713	These results provide important information on rec-eCG in the regulation of specific glycosylation sites and improve our understanding of the specific biological activity of rec-eCG glycosylation sites in equidaes.
35141446	0	84	theme	Sites	82:86	arg1	Activity					20:27	Specific Biological Activity	0:27	Specific Biological Activity of Equine Chorionic Gonadotropin (eCG) Glycosylation Sites in Cells Expressing Equine Luteinizing Hormone/CG (eLH/CG) Receptor	0:154	Specific Biological Activity of Equine Chorionic Gonadotropin (eCG) Glycosylation Sites in Cells Expressing Equine Luteinizing Hormone/CG (eLH/CG) Receptor.
35141446	1	85	theme	dual	311:314	arg1	effects					390:396	dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects	311:396	dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects	311:396	Equine chorionic gonadotropin (eCG), produced by the endometrial cups of the placenta after the first trimester, is a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species.
35141446	1	86	gly	glycoprotein	284:295	arg1	glycoprotein					284:295	a specific glycoprotein	273:295	a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species	273:417	Equine chorionic gonadotropin (eCG), produced by the endometrial cups of the placenta after the first trimester, is a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species.
35141446	1	86	gly	glycoprotein	284:295	arg1	gonadotropin					174:185	Equine chorionic gonadotropin	157:185	Equine chorionic gonadotropin (eCG)	157:191	Equine chorionic gonadotropin (eCG), produced by the endometrial cups of the placenta after the first trimester, is a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species.
35141446	9	87	theme	rec-eCG	1675:1681	arg1	sites					1697:1701	rec-eCG glycosylation sites	1675:1701	rec-eCG glycosylation sites in equidaes	1675:1713	These results provide important information on rec-eCG in the regulation of specific glycosylation sites and improve our understanding of the specific biological activity of rec-eCG glycosylation sites in equidaes.
35141446	3	88	theme	double	788:793	arg1	mutant					795:800	double mutant	788:800	double mutant	788:800	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	88	theme	double	788:793	arg1	mutants					588:594	the following site mutants	569:594	the following site mutants of N- and O-linked glycosylation	569:627	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	7	89	theme	wild-type	1303:1311	arg1	eCG					1313:1315	wild-type eCG	1303:1315	wild-type eCG (141.9 nmol/104 cells)	1303:1338	The Rmax values of the mutants were 56%-80% those of wild-type eCG (141.9 nmol/104 cells).
35141446	7	89	theme	wild-type	1303:1311	arg1	cells					1333:1337	141.9 nmol/104 cells	1318:1337	141.9 nmol/104 cells	1318:1337	The Rmax values of the mutants were 56%-80% those of wild-type eCG (141.9 nmol/104 cells).
35141446	4	90	theme	CHO-S	887:891	arg1	cells					894:898	Chinese hamster ovary suspension (CHO-S) cells	853:898	Chinese hamster ovary suspension (CHO-S) cells	853:898	We produced recombinant eCG (rec-eCG) proteins in Chinese hamster ovary suspension (CHO-S) cells.
35141446	0	91	theme	Equine	108:113	arg1	Receptor					147:154	Equine Luteinizing Hormone/CG (eLH/CG) Receptor	108:154	Equine Luteinizing Hormone/CG (eLH/CG) Receptor	108:154	Specific Biological Activity of Equine Chorionic Gonadotropin (eCG) Glycosylation Sites in Cells Expressing Equine Luteinizing Hormone/CG (eLH/CG) Receptor.
35141446	9	92	from	information	1533:1543	arg1	rec-eCG					1548:1554	rec-eCG	1548:1554	rec-eCG in the regulation of specific glycosylation sites	1548:1604	These results provide important information on rec-eCG in the regulation of specific glycosylation sites and improve our understanding of the specific biological activity of rec-eCG glycosylation sites in equidaes.
35141446	2	93	theme	LH-like	460:466	arg1	activity					468:475	only LH-like activity	455:475	only LH-like activity	455:475	However, in equidaes, eCG exhibits only LH-like activity.
35141446	0	94	theme	Biological	9:18	arg1	Activity					20:27	Specific Biological Activity	0:27	Specific Biological Activity of Equine Chorionic Gonadotropin (eCG) Glycosylation Sites in Cells Expressing Equine Luteinizing Hormone/CG (eLH/CG) Receptor	0:154	Specific Biological Activity of Equine Chorionic Gonadotropin (eCG) Glycosylation Sites in Cells Expressing Equine Luteinizing Hormone/CG (eLH/CG) Receptor.
35141446	5	95	theme	mutants	1068:1074	arg1	activities					1039:1048	signal transduction activities	1019:1048	signal transduction activities of deglycosylated mutants	1019:1074	We examined the biological activity of rec-eCG proteins in CHO-K1 cells expressing the eLH/CG receptor and found that signal transduction activities of deglycosylated mutants remarkably decreased.
35141446	8	96	gly	glycosylation	1452:1464	arg2	sites					1466:1470	O-linked glycosylation sites	1443:1470	O-linked glycosylation sites in cells expressing eLH/CGR	1443:1498	Our results indicate that the biological activity of eCG is greatly affected by the removal of N- and O-linked glycosylation sites in cells expressing eLH/CGR.
35141446	8	97	theme	N-	1436:1437	arg1	removal					1425:1431	the removal	1421:1431	the removal of N- and O-linked glycosylation sites in cells expressing eLH/CGR	1421:1498	Our results indicate that the biological activity of eCG is greatly affected by the removal of N- and O-linked glycosylation sites in cells expressing eLH/CGR.
35141446	9	98	theme	important	1523:1531	arg1	information					1533:1543	important information	1523:1543	important information on rec-eCG in the regulation of specific glycosylation sites	1523:1604	These results provide important information on rec-eCG in the regulation of specific glycosylation sites and improve our understanding of the specific biological activity of rec-eCG glycosylation sites in equidaes.
35141446	3	99	gly	glycosylation	678:690	arg2	site					692:695	α-subunit56 N-linked glycosylation site	657:695	α-subunit56 N-linked glycosylation site	657:695	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	99	gly	glycosylation	678:690	arg2	α-subunit56					657:667	α-subunit56	657:667	α-subunit56	657:667	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	100	from	eCGβ-D/αΔ56	775:785	arg1	β-subunit					760:768	the β-subunit	756:768	the β-subunit	756:768	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	101	theme	N-	599:600	arg1	deletion					708:715	deletion	708:715	deletion of the O-linked glycosylation sites at the β-subunit	708:768	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	101	theme	N-	599:600	arg1	eCGβ-D/αΔ56					775:785	eCGβ-D/αΔ56	775:785	eCGβ-D/αΔ56	775:785	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	101	theme	N-	599:600	arg1	mutants					588:594	the following site mutants	569:594	the following site mutants of N- and O-linked glycosylation	569:627	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	101	theme	N-	599:600	arg1	mutant					795:800	double mutant	788:800	double mutant	788:800	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	3	101	theme	N-	599:600	arg1	eCGβ-D/α					698:705	eCGβ-D/α	698:705	eCGβ-D/α	698:705	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	0	102	theme	Chorionic	39:47	arg1	eCG					63:65	eCG	63:65	eCG	63:65	Specific Biological Activity of Equine Chorionic Gonadotropin (eCG) Glycosylation Sites in Cells Expressing Equine Luteinizing Hormone/CG (eLH/CG) Receptor.
35141446	0	102	theme	Chorionic	39:47	arg1	Gonadotropin					49:60	Equine Chorionic Gonadotropin	32:60	Equine Chorionic Gonadotropin (eCG) Glycosylation Sites	32:86	Specific Biological Activity of Equine Chorionic Gonadotropin (eCG) Glycosylation Sites in Cells Expressing Equine Luteinizing Hormone/CG (eLH/CG) Receptor.
35141446	9	103	from	equidaes	1706:1713	arg1	activity					1663:1670	the specific biological activity	1639:1670	the specific biological activity of rec-eCG glycosylation sites in equidaes	1639:1713	These results provide important information on rec-eCG in the regulation of specific glycosylation sites and improve our understanding of the specific biological activity of rec-eCG glycosylation sites in equidaes.
35141446	8	104	theme	sites	1466:1470	arg1	removal					1425:1431	the removal	1421:1431	the removal of N- and O-linked glycosylation sites in cells expressing eLH/CGR	1421:1498	Our results indicate that the biological activity of eCG is greatly affected by the removal of N- and O-linked glycosylation sites in cells expressing eLH/CGR.
35141446	1	105	theme	specific	275:282	arg1	glycoprotein					284:295	a specific glycoprotein	273:295	a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species	273:417	Equine chorionic gonadotropin (eCG), produced by the endometrial cups of the placenta after the first trimester, is a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species.
35141446	1	105	theme	specific	275:282	arg1	gonadotropin					174:185	Equine chorionic gonadotropin	157:185	Equine chorionic gonadotropin (eCG)	157:191	Equine chorionic gonadotropin (eCG), produced by the endometrial cups of the placenta after the first trimester, is a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species.
35141446	9	106	from	sites	1697:1701	arg1	equidaes					1706:1713	equidaes	1706:1713	equidaes	1706:1713	These results provide important information on rec-eCG in the regulation of specific glycosylation sites and improve our understanding of the specific biological activity of rec-eCG glycosylation sites in equidaes.
35141446	3	107	theme	O-linked	606:613	arg1	glycosylation					615:627	O-linked glycosylation	606:627	O-linked glycosylation	606:627	To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit56 N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites at the β-subunit, and eCGβ-D/αΔ56, double mutant.
35141446	1	108	theme	endometrial	210:220	arg1	cups					222:225	the endometrial cups	206:225	the endometrial cups of the placenta	206:241	Equine chorionic gonadotropin (eCG), produced by the endometrial cups of the placenta after the first trimester, is a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species.
35141446	9	109	from	activity	1663:1670	arg1	equidaes					1706:1713	equidaes	1706:1713	equidaes	1706:1713	These results provide important information on rec-eCG in the regulation of specific glycosylation sites and improve our understanding of the specific biological activity of rec-eCG glycosylation sites in equidaes.
35141446	5	110	theme	rec-eCG	940:946	arg1	proteins					948:955	rec-eCG proteins	940:955	rec-eCG proteins	940:955	We examined the biological activity of rec-eCG proteins in CHO-K1 cells expressing the eLH/CG receptor and found that signal transduction activities of deglycosylated mutants remarkably decreased.
33166890	0	0	theme	high-quality	99:110	arg1	dispersion					112:121	cellulose-assisted high-quality dispersion	80:121	cellulose-assisted high-quality dispersion of carbon nanotubes	80:141	High-strength, blood-compatible, and high-capacity bilirubin adsorbent based on cellulose-assisted high-quality dispersion of carbon nanotubes.
33166890	7	1	dep	demonstrate	1095:1105	arg1	have					1113:1116	have	1113:1116	demonstrate LCCMs have high bilirubin adsorption capacity (204.12 mg/g) that is significantly higher than most of the reported adsorbents	1095:1231	Experimental results demonstrate LCCMs have high bilirubin adsorption capacity (204.12 mg/g) that is significantly higher than most of the reported adsorbents.
33166890	1	2	theme	body	179:182	arg1	organs					184:189	body organs	179:189	body organs	179:189	Excess bilirubin can accumulate in body organs and has serious effects on human health.
33166890	0	3	theme	cellulose-assisted	80:97	arg1	dispersion					112:121	cellulose-assisted high-quality dispersion	80:121	cellulose-assisted high-quality dispersion of carbon nanotubes	80:141	High-strength, blood-compatible, and high-capacity bilirubin adsorbent based on cellulose-assisted high-quality dispersion of carbon nanotubes.
33166890	7	4	theme	high	1118:1121	arg1	capacity					1144:1151	high bilirubin adsorption capacity	1118:1151	high bilirubin adsorption capacity (204.12 mg/g) that is significantly higher than most of the reported adsorbents	1118:1231	Experimental results demonstrate LCCMs have high bilirubin adsorption capacity (204.12 mg/g) that is significantly higher than most of the reported adsorbents.
33166890	7	4	theme	high	1118:1121	arg1	mg/g					1161:1164	204.12 mg/g	1154:1164	204.12 mg/g	1154:1164	Experimental results demonstrate LCCMs have high bilirubin adsorption capacity (204.12 mg/g) that is significantly higher than most of the reported adsorbents.
33166890	4	5	theme	mechanical	803:812	arg1	strength					814:821	the improved mechanical strength	790:821	the improved mechanical strength	790:821	The obtained cellulose/CNTs solution is applied for the fabrication of cellulose/CNTs microspheres (CCMs), in which cellulose serves as a base material and guarantees the blood compatibility of the composite material, and CNTs contribute to the improved mechanical strength and high adsorption capacity.
33166890	3	6	from	cellulose	433:441	arg1	solution					477:484	NaOH/thiourea aqueous solution	455:484	NaOH/thiourea aqueous solution	455:484	By dispersing cellulose and CNTs in NaOH/thiourea aqueous solution, a homogeneous and stable cellulose/CNTs solution is achieved.
33166890	2	7	theme	cellulose-assisted	286:303	arg1	dispersion					318:327	cellulose-assisted high-quality dispersion	286:327	cellulose-assisted high-quality dispersion of carbon nanotubes (CNTs)	286:354	In this work, a simple engineering strategy, based on cellulose-assisted high-quality dispersion of carbon nanotubes (CNTs), is proposed to produce high-performance bilirubin adsorbents.
33166890	4	8	theme	microspheres	635:646	arg1	fabrication					605:615	the fabrication	601:615	the fabrication of cellulose/CNTs microspheres (CCMs)	601:653	The obtained cellulose/CNTs solution is applied for the fabrication of cellulose/CNTs microspheres (CCMs), in which cellulose serves as a base material and guarantees the blood compatibility of the composite material, and CNTs contribute to the improved mechanical strength and high adsorption capacity.
33166890	7	9	theme	reported	1213:1220	arg1	adsorbents					1222:1231	the reported adsorbents	1209:1231	the reported adsorbents	1209:1231	Experimental results demonstrate LCCMs have high bilirubin adsorption capacity (204.12 mg/g) that is significantly higher than most of the reported adsorbents.
33166890	6	10	theme	large	1004:1008	arg1	area					1018:1021	a large surface area	1002:1021	a large surface area (171.31 m2/g)	1002:1035	The obtained lysine-modified CCMs (LCCMs) exhibit a large surface area (171.31 m2/g) and hierarchically porous structure.
33166890	6	10	theme	large	1004:1008	arg1	m2/g					1031:1034	171.31 m2/g	1024:1034	171.31 m2/g	1024:1034	The obtained lysine-modified CCMs (LCCMs) exhibit a large surface area (171.31 m2/g) and hierarchically porous structure.
33166890	4	11	theme	blood	720:724	arg1	cellulose					665:673	cellulose	665:673	cellulose	665:673	The obtained cellulose/CNTs solution is applied for the fabrication of cellulose/CNTs microspheres (CCMs), in which cellulose serves as a base material and guarantees the blood compatibility of the composite material, and CNTs contribute to the improved mechanical strength and high adsorption capacity.
33166890	4	11	theme	blood	720:724	arg1	compatibility					726:738	the blood compatibility	716:738	the blood compatibility of the composite material	716:764	The obtained cellulose/CNTs solution is applied for the fabrication of cellulose/CNTs microspheres (CCMs), in which cellulose serves as a base material and guarantees the blood compatibility of the composite material, and CNTs contribute to the improved mechanical strength and high adsorption capacity.
33166890	4	11	theme	blood	720:724	arg1	material					692:699	a base material	685:699	a base material	685:699	The obtained cellulose/CNTs solution is applied for the fabrication of cellulose/CNTs microspheres (CCMs), in which cellulose serves as a base material and guarantees the blood compatibility of the composite material, and CNTs contribute to the improved mechanical strength and high adsorption capacity.
33166890	4	12	theme	cellulose/CNTs	620:633	arg1	CCMs					649:652	CCMs	649:652	CCMs	649:652	The obtained cellulose/CNTs solution is applied for the fabrication of cellulose/CNTs microspheres (CCMs), in which cellulose serves as a base material and guarantees the blood compatibility of the composite material, and CNTs contribute to the improved mechanical strength and high adsorption capacity.
33166890	4	12	theme	cellulose/CNTs	620:633	arg1	microspheres					635:646	cellulose/CNTs microspheres	620:646	cellulose/CNTs microspheres (CCMs)	620:653	The obtained cellulose/CNTs solution is applied for the fabrication of cellulose/CNTs microspheres (CCMs), in which cellulose serves as a base material and guarantees the blood compatibility of the composite material, and CNTs contribute to the improved mechanical strength and high adsorption capacity.
33166890	0	13	theme	nanotubes	133:141	arg1	dispersion					112:121	cellulose-assisted high-quality dispersion	80:121	cellulose-assisted high-quality dispersion of carbon nanotubes	80:141	High-strength, blood-compatible, and high-capacity bilirubin adsorbent based on cellulose-assisted high-quality dispersion of carbon nanotubes.
33166890	7	14	theme	bilirubin	1123:1131	arg1	capacity					1144:1151	high bilirubin adsorption capacity	1118:1151	high bilirubin adsorption capacity (204.12 mg/g) that is significantly higher than most of the reported adsorbents	1118:1231	Experimental results demonstrate LCCMs have high bilirubin adsorption capacity (204.12 mg/g) that is significantly higher than most of the reported adsorbents.
33166890	7	14	theme	bilirubin	1123:1131	arg1	mg/g					1161:1164	204.12 mg/g	1154:1164	204.12 mg/g	1154:1164	Experimental results demonstrate LCCMs have high bilirubin adsorption capacity (204.12 mg/g) that is significantly higher than most of the reported adsorbents.
33166890	5	15	theme	adsorption	896:905	arg1	capacity					907:914	adsorption capacity	896:914	adsorption capacity	896:914	To further improve blood compatibility and adsorption capacity, lysine is immobilized on the CCMs.
33166890	4	16	theme	improved	794:801	arg1	strength					814:821	the improved mechanical strength	790:821	the improved mechanical strength	790:821	The obtained cellulose/CNTs solution is applied for the fabrication of cellulose/CNTs microspheres (CCMs), in which cellulose serves as a base material and guarantees the blood compatibility of the composite material, and CNTs contribute to the improved mechanical strength and high adsorption capacity.
33166890	2	17	theme	high-performance	380:395	arg1	adsorbents					407:416	high-performance bilirubin adsorbents	380:416	high-performance bilirubin adsorbents	380:416	In this work, a simple engineering strategy, based on cellulose-assisted high-quality dispersion of carbon nanotubes (CNTs), is proposed to produce high-performance bilirubin adsorbents.
33166890	0	18	theme	carbon	126:131	arg1	nanotubes					133:141	carbon nanotubes	126:141	carbon nanotubes	126:141	High-strength, blood-compatible, and high-capacity bilirubin adsorbent based on cellulose-assisted high-quality dispersion of carbon nanotubes.
33166890	7	19	theme	adsorption	1133:1142	arg1	capacity					1144:1151	high bilirubin adsorption capacity	1118:1151	high bilirubin adsorption capacity (204.12 mg/g) that is significantly higher than most of the reported adsorbents	1118:1231	Experimental results demonstrate LCCMs have high bilirubin adsorption capacity (204.12 mg/g) that is significantly higher than most of the reported adsorbents.
33166890	7	19	theme	adsorption	1133:1142	arg1	mg/g					1161:1164	204.12 mg/g	1154:1164	204.12 mg/g	1154:1164	Experimental results demonstrate LCCMs have high bilirubin adsorption capacity (204.12 mg/g) that is significantly higher than most of the reported adsorbents.
33166890	2	20	theme	bilirubin	397:405	arg1	adsorbents					407:416	high-performance bilirubin adsorbents	380:416	high-performance bilirubin adsorbents	380:416	In this work, a simple engineering strategy, based on cellulose-assisted high-quality dispersion of carbon nanotubes (CNTs), is proposed to produce high-performance bilirubin adsorbents.
33166890	2	21	theme	carbon	332:337	arg1	CNTs					350:353	CNTs	350:353	CNTs	350:353	In this work, a simple engineering strategy, based on cellulose-assisted high-quality dispersion of carbon nanotubes (CNTs), is proposed to produce high-performance bilirubin adsorbents.
33166890	2	21	theme	carbon	332:337	arg1	nanotubes					339:347	carbon nanotubes	332:347	carbon nanotubes (CNTs)	332:354	In this work, a simple engineering strategy, based on cellulose-assisted high-quality dispersion of carbon nanotubes (CNTs), is proposed to produce high-performance bilirubin adsorbents.
33166890	1	22	theme	serious	199:205	arg1	effects					207:213	serious effects	199:213	serious effects	199:213	Excess bilirubin can accumulate in body organs and has serious effects on human health.
33166890	6	23	theme	obtained	956:963	arg1	CCMs					981:984	The obtained lysine-modified CCMs	952:984	The obtained lysine-modified CCMs (LCCMs)	952:992	The obtained lysine-modified CCMs (LCCMs) exhibit a large surface area (171.31 m2/g) and hierarchically porous structure.
33166890	6	23	theme	obtained	956:963	arg1	LCCMs					987:991	LCCMs	987:991	LCCMs	987:991	The obtained lysine-modified CCMs (LCCMs) exhibit a large surface area (171.31 m2/g) and hierarchically porous structure.
33166890	4	24	theme	material	757:764	arg1	cellulose					665:673	cellulose	665:673	cellulose	665:673	The obtained cellulose/CNTs solution is applied for the fabrication of cellulose/CNTs microspheres (CCMs), in which cellulose serves as a base material and guarantees the blood compatibility of the composite material, and CNTs contribute to the improved mechanical strength and high adsorption capacity.
33166890	4	24	theme	material	757:764	arg1	compatibility					726:738	the blood compatibility	716:738	the blood compatibility of the composite material	716:764	The obtained cellulose/CNTs solution is applied for the fabrication of cellulose/CNTs microspheres (CCMs), in which cellulose serves as a base material and guarantees the blood compatibility of the composite material, and CNTs contribute to the improved mechanical strength and high adsorption capacity.
33166890	4	24	theme	material	757:764	arg1	material					692:699	a base material	685:699	a base material	685:699	The obtained cellulose/CNTs solution is applied for the fabrication of cellulose/CNTs microspheres (CCMs), in which cellulose serves as a base material and guarantees the blood compatibility of the composite material, and CNTs contribute to the improved mechanical strength and high adsorption capacity.
33166890	4	25	theme	base	687:690	arg1	cellulose					665:673	cellulose	665:673	cellulose	665:673	The obtained cellulose/CNTs solution is applied for the fabrication of cellulose/CNTs microspheres (CCMs), in which cellulose serves as a base material and guarantees the blood compatibility of the composite material, and CNTs contribute to the improved mechanical strength and high adsorption capacity.
33166890	4	25	theme	base	687:690	arg1	compatibility					726:738	the blood compatibility	716:738	the blood compatibility of the composite material	716:764	The obtained cellulose/CNTs solution is applied for the fabrication of cellulose/CNTs microspheres (CCMs), in which cellulose serves as a base material and guarantees the blood compatibility of the composite material, and CNTs contribute to the improved mechanical strength and high adsorption capacity.
33166890	4	25	theme	base	687:690	arg1	material					692:699	a base material	685:699	a base material	685:699	The obtained cellulose/CNTs solution is applied for the fabrication of cellulose/CNTs microspheres (CCMs), in which cellulose serves as a base material and guarantees the blood compatibility of the composite material, and CNTs contribute to the improved mechanical strength and high adsorption capacity.
33166890	4	26	theme	high	827:830	arg1	capacity					843:850	high adsorption capacity	827:850	high adsorption capacity	827:850	The obtained cellulose/CNTs solution is applied for the fabrication of cellulose/CNTs microspheres (CCMs), in which cellulose serves as a base material and guarantees the blood compatibility of the composite material, and CNTs contribute to the improved mechanical strength and high adsorption capacity.
33166890	6	27	theme	porous	1056:1061	arg1	structure					1063:1071	hierarchically porous structure	1041:1071	hierarchically porous structure	1041:1071	The obtained lysine-modified CCMs (LCCMs) exhibit a large surface area (171.31 m2/g) and hierarchically porous structure.
33166890	2	28	theme	high-quality	305:316	arg1	dispersion					318:327	cellulose-assisted high-quality dispersion	286:327	cellulose-assisted high-quality dispersion of carbon nanotubes (CNTs)	286:354	In this work, a simple engineering strategy, based on cellulose-assisted high-quality dispersion of carbon nanotubes (CNTs), is proposed to produce high-performance bilirubin adsorbents.
33166890	1	29	theme	human	218:222	arg1	health					224:229	human health	218:229	human health	218:229	Excess bilirubin can accumulate in body organs and has serious effects on human health.
33166890	3	30	theme	cellulose/CNTs	512:525	arg1	solution					527:534	a homogeneous and stable cellulose/CNTs solution	487:534	a homogeneous and stable cellulose/CNTs solution	487:534	By dispersing cellulose and CNTs in NaOH/thiourea aqueous solution, a homogeneous and stable cellulose/CNTs solution is achieved.
33166890	7	31	theme	Experimental	1074:1085	arg1	results					1087:1093	Experimental results	1074:1093	Experimental results	1074:1093	Experimental results demonstrate LCCMs have high bilirubin adsorption capacity (204.12 mg/g) that is significantly higher than most of the reported adsorbents.
33166890	5	32	theme	blood	872:876	arg1	compatibility					878:890	blood compatibility	872:890	blood compatibility	872:890	To further improve blood compatibility and adsorption capacity, lysine is immobilized on the CCMs.
33166890	8	33	theme	blood	1268:1272	arg1	compatibility					1274:1286	blood compatibility	1268:1286	blood compatibility	1268:1286	The combination of high strength, blood compatibility, and high adsorption capacity positions the LCCMs as a promising candidate for bilirubin removal.
33166890	3	34	theme	aqueous	469:475	arg1	solution					477:484	NaOH/thiourea aqueous solution	455:484	NaOH/thiourea aqueous solution	455:484	By dispersing cellulose and CNTs in NaOH/thiourea aqueous solution, a homogeneous and stable cellulose/CNTs solution is achieved.
33166890	8	35	theme	strength	1258:1265	arg1	combination					1238:1248	The combination	1234:1248	The combination of high strength, blood compatibility, and high adsorption capacity	1234:1316	The combination of high strength, blood compatibility, and high adsorption capacity positions the LCCMs as a promising candidate for bilirubin removal.
33166890	4	36	theme	obtained	553:560	arg1	solution					577:584	The obtained cellulose/CNTs solution	549:584	The obtained cellulose/CNTs solution	549:584	The obtained cellulose/CNTs solution is applied for the fabrication of cellulose/CNTs microspheres (CCMs), in which cellulose serves as a base material and guarantees the blood compatibility of the composite material, and CNTs contribute to the improved mechanical strength and high adsorption capacity.
33166890	1	37	contain	has	195:197	arg1	bilirubin					151:159	Excess bilirubin	144:159	Excess bilirubin	144:159	Excess bilirubin can accumulate in body organs and has serious effects on human health.
33166890	1	37	contain	has	195:197	arg2	effects					207:213	serious effects	199:213	serious effects	199:213	Excess bilirubin can accumulate in body organs and has serious effects on human health.
33166890	4	38	theme	adsorption	832:841	arg1	capacity					843:850	high adsorption capacity	827:850	high adsorption capacity	827:850	The obtained cellulose/CNTs solution is applied for the fabrication of cellulose/CNTs microspheres (CCMs), in which cellulose serves as a base material and guarantees the blood compatibility of the composite material, and CNTs contribute to the improved mechanical strength and high adsorption capacity.
33166890	6	39	theme	hierarchically	1041:1054	arg1	structure					1063:1071	hierarchically porous structure	1041:1071	hierarchically porous structure	1041:1071	The obtained lysine-modified CCMs (LCCMs) exhibit a large surface area (171.31 m2/g) and hierarchically porous structure.
33166890	8	40	theme	high	1253:1256	arg1	strength					1258:1265	high strength	1253:1265	high strength	1253:1265	The combination of high strength, blood compatibility, and high adsorption capacity positions the LCCMs as a promising candidate for bilirubin removal.
33166890	8	41	theme	bilirubin	1367:1375	arg1	removal					1377:1383	bilirubin removal	1367:1383	bilirubin removal	1367:1383	The combination of high strength, blood compatibility, and high adsorption capacity positions the LCCMs as a promising candidate for bilirubin removal.
33166890	3	42	theme	stable	505:510	arg1	solution					527:534	a homogeneous and stable cellulose/CNTs solution	487:534	a homogeneous and stable cellulose/CNTs solution	487:534	By dispersing cellulose and CNTs in NaOH/thiourea aqueous solution, a homogeneous and stable cellulose/CNTs solution is achieved.
33166890	6	43	theme	lysine-modified	965:979	arg1	CCMs					981:984	The obtained lysine-modified CCMs	952:984	The obtained lysine-modified CCMs (LCCMs)	952:992	The obtained lysine-modified CCMs (LCCMs) exhibit a large surface area (171.31 m2/g) and hierarchically porous structure.
33166890	6	43	theme	lysine-modified	965:979	arg1	LCCMs					987:991	LCCMs	987:991	LCCMs	987:991	The obtained lysine-modified CCMs (LCCMs) exhibit a large surface area (171.31 m2/g) and hierarchically porous structure.
33166890	8	44	theme	promising	1343:1351	arg1	LCCMs					1332:1336	the LCCMs	1328:1336	the LCCMs	1328:1336	The combination of high strength, blood compatibility, and high adsorption capacity positions the LCCMs as a promising candidate for bilirubin removal.
33166890	8	44	theme	promising	1343:1351	arg1	candidate					1353:1361	a promising candidate	1341:1361	a promising candidate for bilirubin removal	1341:1383	The combination of high strength, blood compatibility, and high adsorption capacity positions the LCCMs as a promising candidate for bilirubin removal.
33166890	1	45	theme	Excess	144:149	arg1	bilirubin					151:159	Excess bilirubin	144:159	Excess bilirubin	144:159	Excess bilirubin can accumulate in body organs and has serious effects on human health.
33166890	4	46	theme	cellulose/CNTs	562:575	arg1	solution					577:584	The obtained cellulose/CNTs solution	549:584	The obtained cellulose/CNTs solution	549:584	The obtained cellulose/CNTs solution is applied for the fabrication of cellulose/CNTs microspheres (CCMs), in which cellulose serves as a base material and guarantees the blood compatibility of the composite material, and CNTs contribute to the improved mechanical strength and high adsorption capacity.
33166890	8	47	theme	adsorption	1298:1307	arg1	capacity					1309:1316	high adsorption capacity	1293:1316	high adsorption capacity	1293:1316	The combination of high strength, blood compatibility, and high adsorption capacity positions the LCCMs as a promising candidate for bilirubin removal.
33166890	8	48	theme	compatibility	1274:1286	arg1	combination					1238:1248	The combination	1234:1248	The combination of high strength, blood compatibility, and high adsorption capacity	1234:1316	The combination of high strength, blood compatibility, and high adsorption capacity positions the LCCMs as a promising candidate for bilirubin removal.
33166890	3	49	theme	homogeneous	489:499	arg1	solution					527:534	a homogeneous and stable cellulose/CNTs solution	487:534	a homogeneous and stable cellulose/CNTs solution	487:534	By dispersing cellulose and CNTs in NaOH/thiourea aqueous solution, a homogeneous and stable cellulose/CNTs solution is achieved.
33166890	2	50	theme	engineering	255:265	arg1	strategy					267:274	a simple engineering strategy	246:274	a simple engineering strategy	246:274	In this work, a simple engineering strategy, based on cellulose-assisted high-quality dispersion of carbon nanotubes (CNTs), is proposed to produce high-performance bilirubin adsorbents.
33166890	2	51	theme	nanotubes	339:347	arg1	dispersion					318:327	cellulose-assisted high-quality dispersion	286:327	cellulose-assisted high-quality dispersion of carbon nanotubes (CNTs)	286:354	In this work, a simple engineering strategy, based on cellulose-assisted high-quality dispersion of carbon nanotubes (CNTs), is proposed to produce high-performance bilirubin adsorbents.
33166890	4	52	theme	composite	747:755	arg1	material					757:764	the composite material	743:764	the composite material	743:764	The obtained cellulose/CNTs solution is applied for the fabrication of cellulose/CNTs microspheres (CCMs), in which cellulose serves as a base material and guarantees the blood compatibility of the composite material, and CNTs contribute to the improved mechanical strength and high adsorption capacity.
33166890	8	53	theme	high	1293:1296	arg1	capacity					1309:1316	high adsorption capacity	1293:1316	high adsorption capacity	1293:1316	The combination of high strength, blood compatibility, and high adsorption capacity positions the LCCMs as a promising candidate for bilirubin removal.
33166890	2	54	theme	simple	248:253	arg1	strategy					267:274	a simple engineering strategy	246:274	a simple engineering strategy	246:274	In this work, a simple engineering strategy, based on cellulose-assisted high-quality dispersion of carbon nanotubes (CNTs), is proposed to produce high-performance bilirubin adsorbents.
33166890	3	55	from	CNTs	447:450	arg1	solution					477:484	NaOH/thiourea aqueous solution	455:484	NaOH/thiourea aqueous solution	455:484	By dispersing cellulose and CNTs in NaOH/thiourea aqueous solution, a homogeneous and stable cellulose/CNTs solution is achieved.
33166890	3	56	theme	NaOH/thiourea	455:467	arg1	solution					477:484	NaOH/thiourea aqueous solution	455:484	NaOH/thiourea aqueous solution	455:484	By dispersing cellulose and CNTs in NaOH/thiourea aqueous solution, a homogeneous and stable cellulose/CNTs solution is achieved.
33166890	6	57	theme	surface	1010:1016	arg1	area					1018:1021	a large surface area	1002:1021	a large surface area (171.31 m2/g)	1002:1035	The obtained lysine-modified CCMs (LCCMs) exhibit a large surface area (171.31 m2/g) and hierarchically porous structure.
33166890	6	57	theme	surface	1010:1016	arg1	m2/g					1031:1034	171.31 m2/g	1024:1034	171.31 m2/g	1024:1034	The obtained lysine-modified CCMs (LCCMs) exhibit a large surface area (171.31 m2/g) and hierarchically porous structure.
33166890	8	58	theme	capacity	1309:1316	arg1	combination					1238:1248	The combination	1234:1248	The combination of high strength, blood compatibility, and high adsorption capacity	1234:1316	The combination of high strength, blood compatibility, and high adsorption capacity positions the LCCMs as a promising candidate for bilirubin removal.
33362841	2	0	contain	contain	425:431	arg2	sequences					440:448	signal sequences	433:448	signal sequences for addition of a glycosylphosphatidylinositol (GPI)-anchor to tether them to the plasma membrane	433:546	In addition to FAS1 domains, these multi-domain FLA proteins contain glycomotif regions predicted to direct addition of large arabinogalactan (AG) glycans and many contain signal sequences for addition of a glycosylphosphatidylinositol (GPI)-anchor to tether them to the plasma membrane.
33362841	2	0	contain	contain	425:431	arg1	many					420:423	many	420:423	many	420:423	In addition to FAS1 domains, these multi-domain FLA proteins contain glycomotif regions predicted to direct addition of large arabinogalactan (AG) glycans and many contain signal sequences for addition of a glycosylphosphatidylinositol (GPI)-anchor to tether them to the plasma membrane.
33362841	11	1	theme	wall	1845:1848	arg1	synthesis					1850:1858	plant secondary cell wall synthesis	1824:1858	plant secondary cell wall synthesis	1824:1858	Together these results indicate FLA16, a two-FAS1 domain FLAs, plays a role in plant secondary cell wall synthesis and function.
33362841	8	2	gly	glycosylation	1419:1431	arg1	glycoproteins					1391:1403	cell wall glycoproteins	1381:1403	cell wall glycoproteins with moderate glycosylation	1381:1431	A FLA16-YFP fusion protein driven by the endogenous putative FLA16 promoter in wild type background showed expression in cells with secondary cell walls, and FLA16 displayed characteristics of cell wall glycoproteins with moderate glycosylation.
33362841	3	3	theme	FAS1-containing	696:710	arg1	proteins					712:719	FAS1-containing proteins	696:719	FAS1-containing proteins in animals	696:730	FLAs are proposed to play both structural and signaling functions by forming a range of interactions in the plant extracellular matrix, similar to FAS1-containing proteins in animals.
33362841	8	4	gly	glycoproteins	1391:1403	arg1	glycoproteins					1391:1403	cell wall glycoproteins	1381:1403	cell wall glycoproteins with moderate glycosylation	1381:1431	A FLA16-YFP fusion protein driven by the endogenous putative FLA16 promoter in wild type background showed expression in cells with secondary cell walls, and FLA16 displayed characteristics of cell wall glycoproteins with moderate glycosylation.
33362841	10	5	theme	fiber	1732:1736	arg1	cells					1738:1742	interfascicular stem fiber cells	1711:1742	interfascicular stem fiber cells	1711:1742	Immuno-labeling indicated support for FLA16 location to the plasma-membrane and (apoplastic) cell wall of interfascicular stem fiber cells.
33362841	5	6	theme	B	838:838	arg1	members					840:846	the group B members	828:846	the group B members	828:846	None of the group B members have been functionally characterized or their sub-cellular location resolved, limiting understanding of their function.
33362841	3	7	from	proteins	712:719	arg1	animals					724:730	animals	724:730	animals	724:730	FLAs are proposed to play both structural and signaling functions by forming a range of interactions in the plant extracellular matrix, similar to FAS1-containing proteins in animals.
33362841	11	8	theme	two-FAS1	1786:1793	arg1	FLAs					1802:1805	a two-FAS1 domain FLAs	1784:1805	a two-FAS1 domain FLAs	1784:1805	Together these results indicate FLA16, a two-FAS1 domain FLAs, plays a role in plant secondary cell wall synthesis and function.
33362841	11	8	theme	two-FAS1	1786:1793	arg1	FLA16					1777:1781	FLA16	1777:1781	FLA16	1777:1781	Together these results indicate FLA16, a two-FAS1 domain FLAs, plays a role in plant secondary cell wall synthesis and function.
33362841	2	9	theme	FLA	309:311	arg1	proteins					313:320	these multi-domain FLA proteins	290:320	these multi-domain FLA proteins	290:320	In addition to FAS1 domains, these multi-domain FLA proteins contain glycomotif regions predicted to direct addition of large arabinogalactan (AG) glycans and many contain signal sequences for addition of a glycosylphosphatidylinositol (GPI)-anchor to tether them to the plasma membrane.
33362841	8	10	theme	putative	1240:1247	arg1	promoter					1255:1262	the endogenous putative FLA16 promoter	1225:1262	the endogenous putative FLA16 promoter in wild type background	1225:1286	A FLA16-YFP fusion protein driven by the endogenous putative FLA16 promoter in wild type background showed expression in cells with secondary cell walls, and FLA16 displayed characteristics of cell wall glycoproteins with moderate glycosylation.
33362841	1	11	theme	Arabinogalactan-protein	192:214	arg1	family					222:227	the Fasciclin-Like Arabinogalactan-protein (FLA) family	173:227	the Fasciclin-Like Arabinogalactan-protein (FLA) family of extracellular glycoproteins	173:258	The predominant Fascilin 1 (FAS1)-containing proteins in plants belong to the Fasciclin-Like Arabinogalactan-protein (FLA) family of extracellular glycoproteins.
33362841	5	12	theme	sub-cellular	894:905	arg1	location					907:914	their sub-cellular location	888:914	their sub-cellular location	888:914	None of the group B members have been functionally characterized or their sub-cellular location resolved, limiting understanding of their function.
33362841	2	13	theme	plasma	532:537	arg1	membrane					539:546	the plasma membrane	528:546	the plasma membrane	528:546	In addition to FAS1 domains, these multi-domain FLA proteins contain glycomotif regions predicted to direct addition of large arabinogalactan (AG) glycans and many contain signal sequences for addition of a glycosylphosphatidylinositol (GPI)-anchor to tether them to the plasma membrane.
33362841	0	14	from	Development	71:81	arg1	Arabidopsis					86:96	Arabidopsis	86:96	Arabidopsis	86:96	Fasciclin-Like Arabinogalactan-Protein 16 (FLA16) Is Required for Stem Development in Arabidopsis.
33362841	1	15	theme	FLA	217:219	arg1	family					222:227	the Fasciclin-Like Arabinogalactan-protein (FLA) family	173:227	the Fasciclin-Like Arabinogalactan-protein (FLA) family of extracellular glycoproteins	173:258	The predominant Fascilin 1 (FAS1)-containing proteins in plants belong to the Fasciclin-Like Arabinogalactan-protein (FLA) family of extracellular glycoproteins.
33362841	8	16	theme	fusion	1200:1205	arg1	protein					1207:1213	A FLA16-YFP fusion protein	1188:1213	A FLA16-YFP fusion protein driven by the endogenous putative FLA16 promoter in wild type background	1188:1286	A FLA16-YFP fusion protein driven by the endogenous putative FLA16 promoter in wild type background showed expression in cells with secondary cell walls, and FLA16 displayed characteristics of cell wall glycoproteins with moderate glycosylation.
33362841	8	17	theme	wild	1267:1270	arg1	background					1277:1286	wild type background	1267:1286	wild type background	1267:1286	A FLA16-YFP fusion protein driven by the endogenous putative FLA16 promoter in wild type background showed expression in cells with secondary cell walls, and FLA16 displayed characteristics of cell wall glycoproteins with moderate glycosylation.
33362841	9	18	theme	FLA16	1481:1485	arg1	loss					1473:1476	loss	1473:1476	loss of FLA16	1473:1485	Investigation of a fla16 mutant showed loss of FLA16 leads to reduced stem length and altered biomechanical properties, likely as a result of reduced levels of cellulose.
33362841	8	19	from	promoter	1255:1262	arg1	background					1277:1286	wild type background	1267:1286	wild type background	1267:1286	A FLA16-YFP fusion protein driven by the endogenous putative FLA16 promoter in wild type background showed expression in cells with secondary cell walls, and FLA16 displayed characteristics of cell wall glycoproteins with moderate glycosylation.
33362841	2	20	theme	-anchor	502:508	arg1	GPI					498:500	GPI	498:500	GPI	498:500	In addition to FAS1 domains, these multi-domain FLA proteins contain glycomotif regions predicted to direct addition of large arabinogalactan (AG) glycans and many contain signal sequences for addition of a glycosylphosphatidylinositol (GPI)-anchor to tether them to the plasma membrane.
33362841	2	20	theme	-anchor	502:508	arg1	glycosylphosphatidylinositol					468:495	a glycosylphosphatidylinositol	466:495	a glycosylphosphatidylinositol (GPI)-anchor to tether them to the plasma membrane	466:546	In addition to FAS1 domains, these multi-domain FLA proteins contain glycomotif regions predicted to direct addition of large arabinogalactan (AG) glycans and many contain signal sequences for addition of a glycosylphosphatidylinositol (GPI)-anchor to tether them to the plasma membrane.
33362841	2	21	contain	contain	322:328	arg2	regions					341:347	glycomotif regions	330:347	glycomotif regions predicted to direct addition of large arabinogalactan (AG) glycans	330:414	In addition to FAS1 domains, these multi-domain FLA proteins contain glycomotif regions predicted to direct addition of large arabinogalactan (AG) glycans and many contain signal sequences for addition of a glycosylphosphatidylinositol (GPI)-anchor to tether them to the plasma membrane.
33362841	2	21	contain	contain	322:328	arg1	proteins					313:320	these multi-domain FLA proteins	290:320	these multi-domain FLA proteins	290:320	In addition to FAS1 domains, these multi-domain FLA proteins contain glycomotif regions predicted to direct addition of large arabinogalactan (AG) glycans and many contain signal sequences for addition of a glycosylphosphatidylinositol (GPI)-anchor to tether them to the plasma membrane.
33362841	9	22	theme	stem	1504:1507	arg1	length					1509:1514	reduced stem length	1496:1514	reduced stem length	1496:1514	Investigation of a fla16 mutant showed loss of FLA16 leads to reduced stem length and altered biomechanical properties, likely as a result of reduced levels of cellulose.
33362841	7	23	dep	members	1140:1146	arg1	FLA11					1148:1152	FLA11	1148:1152	FLA11	1148:1152	FLA16 is the most highly expressed FLA in the stem after Group A members FLA11 and FLA12 that are stem specific.
33362841	7	23	dep	members	1140:1146	arg1	members					1140:1146	Group A members FLA11 and FLA12	1132:1162	Group A members FLA11 and FLA12	1132:1162	FLA16 is the most highly expressed FLA in the stem after Group A members FLA11 and FLA12 that are stem specific.
33362841	7	23	dep	members	1140:1146	arg1	FLA12					1158:1162	FLA12	1158:1162	FLA12	1158:1162	FLA16 is the most highly expressed FLA in the stem after Group A members FLA11 and FLA12 that are stem specific.
33362841	1	24	from	proteins	144:151	arg1	plants					156:161	plants	156:161	plants	156:161	The predominant Fascilin 1 (FAS1)-containing proteins in plants belong to the Fasciclin-Like Arabinogalactan-protein (FLA) family of extracellular glycoproteins.
33362841	6	25	theme	group	988:992	arg1	FLA16					996:1000	the group B FLA16	984:1000	the group B FLA16 in Arabidopsis that is predominantly expressed in inflorescence tissues	984:1072	We investigated the group B FLA16 in Arabidopsis that is predominantly expressed in inflorescence tissues.
33362841	8	26	theme	secondary	1320:1328	arg1	walls					1335:1339	secondary cell walls	1320:1339	secondary cell walls	1320:1339	A FLA16-YFP fusion protein driven by the endogenous putative FLA16 promoter in wild type background showed expression in cells with secondary cell walls, and FLA16 displayed characteristics of cell wall glycoproteins with moderate glycosylation.
33362841	9	27	theme	biomechanical	1528:1540	arg1	properties					1542:1551	altered biomechanical properties	1520:1551	altered biomechanical properties	1520:1551	Investigation of a fla16 mutant showed loss of FLA16 leads to reduced stem length and altered biomechanical properties, likely as a result of reduced levels of cellulose.
33362841	4	28	theme	FAS1	765:768	arg1	domains					770:776	two FAS1 domains	761:776	two FAS1 domains	761:776	FLA group B members contain two FAS1 domains and are not predicted to be GPI-anchored.
33362841	4	29	theme	FLA	733:735	arg1	members					745:751	FLA group B members	733:751	FLA group B members	733:751	FLA group B members contain two FAS1 domains and are not predicted to be GPI-anchored.
33362841	2	30	theme	arabinogalactan	387:401	arg1	glycans					408:414	large arabinogalactan (AG) glycans	381:414	large arabinogalactan (AG) glycans	381:414	In addition to FAS1 domains, these multi-domain FLA proteins contain glycomotif regions predicted to direct addition of large arabinogalactan (AG) glycans and many contain signal sequences for addition of a glycosylphosphatidylinositol (GPI)-anchor to tether them to the plasma membrane.
33362841	0	31	theme	Fasciclin-Like	0:13	arg1	Arabinogalactan-Protein					15:37	Fasciclin-Like Arabinogalactan-Protein 16	0:40	Fasciclin-Like Arabinogalactan-Protein 16 (FLA16)	0:48	Fasciclin-Like Arabinogalactan-Protein 16 (FLA16) Is Required for Stem Development in Arabidopsis.
33362841	0	31	theme	Fasciclin-Like	0:13	arg1	FLA16					43:47	FLA16	43:47	FLA16	43:47	Fasciclin-Like Arabinogalactan-Protein 16 (FLA16) Is Required for Stem Development in Arabidopsis.
33362841	8	32	from	expression	1295:1304	arg1	cells					1309:1313	cells	1309:1313	cells with secondary cell walls	1309:1339	A FLA16-YFP fusion protein driven by the endogenous putative FLA16 promoter in wild type background showed expression in cells with secondary cell walls, and FLA16 displayed characteristics of cell wall glycoproteins with moderate glycosylation.
33362841	6	33	theme	inflorescence	1052:1064	arg1	tissues					1066:1072	inflorescence tissues	1052:1072	inflorescence tissues	1052:1072	We investigated the group B FLA16 in Arabidopsis that is predominantly expressed in inflorescence tissues.
33362841	2	34	theme	signal	433:438	arg1	sequences					440:448	signal sequences	433:448	signal sequences for addition of a glycosylphosphatidylinositol (GPI)-anchor to tether them to the plasma membrane	433:546	In addition to FAS1 domains, these multi-domain FLA proteins contain glycomotif regions predicted to direct addition of large arabinogalactan (AG) glycans and many contain signal sequences for addition of a glycosylphosphatidylinositol (GPI)-anchor to tether them to the plasma membrane.
33362841	4	35	theme	B	743:743	arg1	members					745:751	FLA group B members	733:751	FLA group B members	733:751	FLA group B members contain two FAS1 domains and are not predicted to be GPI-anchored.
33362841	10	36	theme	cells	1738:1742	arg1	plasma-membrane					1665:1679	the plasma-membrane and (apoplastic) cell wall	1661:1706	plasma-membrane	1665:1679	Immuno-labeling indicated support for FLA16 location to the plasma-membrane and (apoplastic) cell wall of interfascicular stem fiber cells.
33362841	10	36	theme	cells	1738:1742	arg1	wall					1703:1706	the plasma-membrane and (apoplastic) cell wall	1661:1706	wall	1703:1706	Immuno-labeling indicated support for FLA16 location to the plasma-membrane and (apoplastic) cell wall of interfascicular stem fiber cells.
33362841	1	37	theme	predominant	103:113	arg1	proteins					144:151	The predominant Fascilin 1 (FAS1)-containing proteins	99:151	The predominant Fascilin 1 (FAS1)-containing proteins in plants	99:161	The predominant Fascilin 1 (FAS1)-containing proteins in plants belong to the Fasciclin-Like Arabinogalactan-protein (FLA) family of extracellular glycoproteins.
33362841	2	38	theme	glycosylphosphatidylinositol	468:495	arg1	addition					454:461	addition	454:461	addition of a glycosylphosphatidylinositol (GPI)-anchor to tether them to the plasma membrane	454:546	In addition to FAS1 domains, these multi-domain FLA proteins contain glycomotif regions predicted to direct addition of large arabinogalactan (AG) glycans and many contain signal sequences for addition of a glycosylphosphatidylinositol (GPI)-anchor to tether them to the plasma membrane.
33362841	3	39	theme	interactions	637:648	arg1	range					628:632	a range	626:632	a range of interactions	626:648	FLAs are proposed to play both structural and signaling functions by forming a range of interactions in the plant extracellular matrix, similar to FAS1-containing proteins in animals.
33362841	2	40	theme	glycans	408:414	arg1	addition					369:376	addition	369:376	addition of large arabinogalactan (AG) glycans	369:414	In addition to FAS1 domains, these multi-domain FLA proteins contain glycomotif regions predicted to direct addition of large arabinogalactan (AG) glycans and many contain signal sequences for addition of a glycosylphosphatidylinositol (GPI)-anchor to tether them to the plasma membrane.
33362841	7	41	theme	A	1138:1138	arg1	FLA11					1148:1152	FLA11	1148:1152	FLA11	1148:1152	FLA16 is the most highly expressed FLA in the stem after Group A members FLA11 and FLA12 that are stem specific.
33362841	7	41	theme	A	1138:1138	arg1	members					1140:1146	Group A members FLA11 and FLA12	1132:1162	Group A members FLA11 and FLA12	1132:1162	FLA16 is the most highly expressed FLA in the stem after Group A members FLA11 and FLA12 that are stem specific.
33362841	7	41	theme	A	1138:1138	arg1	FLA12					1158:1162	FLA12	1158:1162	FLA12	1158:1162	FLA16 is the most highly expressed FLA in the stem after Group A members FLA11 and FLA12 that are stem specific.
33362841	9	42	theme	reduced	1576:1582	arg1	levels					1584:1589	reduced levels	1576:1589	reduced levels of cellulose	1576:1602	Investigation of a fla16 mutant showed loss of FLA16 leads to reduced stem length and altered biomechanical properties, likely as a result of reduced levels of cellulose.
33362841	7	43	from	FLA	1110:1112	arg1	stem					1121:1124	the stem	1117:1124	the stem after Group A members FLA11 and FLA12	1117:1162	FLA16 is the most highly expressed FLA in the stem after Group A members FLA11 and FLA12 that are stem specific.
33362841	8	44	theme	wall	1386:1389	arg1	glycoproteins					1391:1403	cell wall glycoproteins	1381:1403	cell wall glycoproteins with moderate glycosylation	1381:1431	A FLA16-YFP fusion protein driven by the endogenous putative FLA16 promoter in wild type background showed expression in cells with secondary cell walls, and FLA16 displayed characteristics of cell wall glycoproteins with moderate glycosylation.
33362841	10	45	theme	cell	1698:1701	arg1	wall					1703:1706	the plasma-membrane and (apoplastic) cell wall	1661:1706	wall	1703:1706	Immuno-labeling indicated support for FLA16 location to the plasma-membrane and (apoplastic) cell wall of interfascicular stem fiber cells.
33362841	3	46	theme	extracellular	663:675	arg1	matrix					677:682	the plant extracellular matrix	653:682	the plant extracellular matrix	653:682	FLAs are proposed to play both structural and signaling functions by forming a range of interactions in the plant extracellular matrix, similar to FAS1-containing proteins in animals.
33362841	2	47	theme	glycomotif	330:339	arg1	regions					341:347	glycomotif regions	330:347	glycomotif regions predicted to direct addition of large arabinogalactan (AG) glycans	330:414	In addition to FAS1 domains, these multi-domain FLA proteins contain glycomotif regions predicted to direct addition of large arabinogalactan (AG) glycans and many contain signal sequences for addition of a glycosylphosphatidylinositol (GPI)-anchor to tether them to the plasma membrane.
33362841	1	48	theme	-containing	132:142	arg1	proteins					144:151	The predominant Fascilin 1 (FAS1)-containing proteins	99:151	The predominant Fascilin 1 (FAS1)-containing proteins in plants	99:161	The predominant Fascilin 1 (FAS1)-containing proteins in plants belong to the Fasciclin-Like Arabinogalactan-protein (FLA) family of extracellular glycoproteins.
33362841	10	49	theme	stem	1727:1730	arg1	cells					1738:1742	interfascicular stem fiber cells	1711:1742	interfascicular stem fiber cells	1711:1742	Immuno-labeling indicated support for FLA16 location to the plasma-membrane and (apoplastic) cell wall of interfascicular stem fiber cells.
33362841	11	50	theme	cell	1840:1843	arg1	synthesis					1850:1858	plant secondary cell wall synthesis	1824:1858	plant secondary cell wall synthesis	1824:1858	Together these results indicate FLA16, a two-FAS1 domain FLAs, plays a role in plant secondary cell wall synthesis and function.
33362841	1	51	theme	glycoproteins	246:258	arg1	family					222:227	the Fasciclin-Like Arabinogalactan-protein (FLA) family	173:227	the Fasciclin-Like Arabinogalactan-protein (FLA) family of extracellular glycoproteins	173:258	The predominant Fascilin 1 (FAS1)-containing proteins in plants belong to the Fasciclin-Like Arabinogalactan-protein (FLA) family of extracellular glycoproteins.
33362841	7	52	theme	expressed	1100:1108	arg1	FLA					1110:1112	the most highly expressed FLA	1084:1112	the most highly expressed FLA in the stem after Group A members FLA11 and FLA12 that are stem specific	1084:1185	FLA16 is the most highly expressed FLA in the stem after Group A members FLA11 and FLA12 that are stem specific.
33362841	7	52	theme	expressed	1100:1108	arg1	specific					1178:1185	specific	1178:1185	specific	1178:1185	FLA16 is the most highly expressed FLA in the stem after Group A members FLA11 and FLA12 that are stem specific.
33362841	7	52	theme	expressed	1100:1108	arg1	FLA16					1075:1079	FLA16	1075:1079	FLA16	1075:1079	FLA16 is the most highly expressed FLA in the stem after Group A members FLA11 and FLA12 that are stem specific.
33362841	2	53	theme	FAS1	276:279	arg1	domains					281:287	FAS1 domains	276:287	FAS1 domains	276:287	In addition to FAS1 domains, these multi-domain FLA proteins contain glycomotif regions predicted to direct addition of large arabinogalactan (AG) glycans and many contain signal sequences for addition of a glycosylphosphatidylinositol (GPI)-anchor to tether them to the plasma membrane.
33362841	5	54	theme	group	832:836	arg1	members					840:846	the group B members	828:846	the group B members	828:846	None of the group B members have been functionally characterized or their sub-cellular location resolved, limiting understanding of their function.
33362841	5	55	theme	members	840:846	arg1	None					820:823	None	820:823	None of the group B members	820:846	None of the group B members have been functionally characterized or their sub-cellular location resolved, limiting understanding of their function.
33362841	3	56	theme	plant	657:661	arg1	matrix					677:682	the plant extracellular matrix	653:682	the plant extracellular matrix	653:682	FLAs are proposed to play both structural and signaling functions by forming a range of interactions in the plant extracellular matrix, similar to FAS1-containing proteins in animals.
33362841	11	57	theme	domain	1795:1800	arg1	FLAs					1802:1805	a two-FAS1 domain FLAs	1784:1805	a two-FAS1 domain FLAs	1784:1805	Together these results indicate FLA16, a two-FAS1 domain FLAs, plays a role in plant secondary cell wall synthesis and function.
33362841	11	57	theme	domain	1795:1800	arg1	FLA16					1777:1781	FLA16	1777:1781	FLA16	1777:1781	Together these results indicate FLA16, a two-FAS1 domain FLAs, plays a role in plant secondary cell wall synthesis and function.
33362841	2	58	theme	multi-domain	296:307	arg1	proteins					313:320	these multi-domain FLA proteins	290:320	these multi-domain FLA proteins	290:320	In addition to FAS1 domains, these multi-domain FLA proteins contain glycomotif regions predicted to direct addition of large arabinogalactan (AG) glycans and many contain signal sequences for addition of a glycosylphosphatidylinositol (GPI)-anchor to tether them to the plasma membrane.
33362841	9	59	theme	fla16	1453:1457	arg1	mutant					1459:1464	a fla16 mutant	1451:1464	a fla16 mutant	1451:1464	Investigation of a fla16 mutant showed loss of FLA16 leads to reduced stem length and altered biomechanical properties, likely as a result of reduced levels of cellulose.
33362841	8	60	theme	endogenous	1229:1238	arg1	promoter					1255:1262	the endogenous putative FLA16 promoter	1225:1262	the endogenous putative FLA16 promoter in wild type background	1225:1286	A FLA16-YFP fusion protein driven by the endogenous putative FLA16 promoter in wild type background showed expression in cells with secondary cell walls, and FLA16 displayed characteristics of cell wall glycoproteins with moderate glycosylation.
33362841	3	61	theme	structural	580:589	arg1	functions					605:613	both structural and signaling functions	575:613	both structural and signaling functions	575:613	FLAs are proposed to play both structural and signaling functions by forming a range of interactions in the plant extracellular matrix, similar to FAS1-containing proteins in animals.
33362841	8	62	theme	FLA16	1249:1253	arg1	promoter					1255:1262	the endogenous putative FLA16 promoter	1225:1262	the endogenous putative FLA16 promoter in wild type background	1225:1286	A FLA16-YFP fusion protein driven by the endogenous putative FLA16 promoter in wild type background showed expression in cells with secondary cell walls, and FLA16 displayed characteristics of cell wall glycoproteins with moderate glycosylation.
33362841	8	63	theme	FLA16-YFP	1190:1198	arg1	protein					1207:1213	A FLA16-YFP fusion protein	1188:1213	A FLA16-YFP fusion protein driven by the endogenous putative FLA16 promoter in wild type background	1188:1286	A FLA16-YFP fusion protein driven by the endogenous putative FLA16 promoter in wild type background showed expression in cells with secondary cell walls, and FLA16 displayed characteristics of cell wall glycoproteins with moderate glycosylation.
33362841	8	64	with	glycoproteins	1391:1403	arg1	glycosylation					1419:1431	moderate glycosylation	1410:1431	moderate glycosylation	1410:1431	A FLA16-YFP fusion protein driven by the endogenous putative FLA16 promoter in wild type background showed expression in cells with secondary cell walls, and FLA16 displayed characteristics of cell wall glycoproteins with moderate glycosylation.
33362841	8	65	theme	type	1272:1275	arg1	background					1277:1286	wild type background	1267:1286	wild type background	1267:1286	A FLA16-YFP fusion protein driven by the endogenous putative FLA16 promoter in wild type background showed expression in cells with secondary cell walls, and FLA16 displayed characteristics of cell wall glycoproteins with moderate glycosylation.
33362841	0	66	theme	Stem	66:69	arg1	Development					71:81	Stem Development	66:81	Stem Development in Arabidopsis	66:96	Fasciclin-Like Arabinogalactan-Protein 16 (FLA16) Is Required for Stem Development in Arabidopsis.
33362841	3	67	theme	signaling	595:603	arg1	functions					605:613	both structural and signaling functions	575:613	both structural and signaling functions	575:613	FLAs are proposed to play both structural and signaling functions by forming a range of interactions in the plant extracellular matrix, similar to FAS1-containing proteins in animals.
33362841	1	68	theme	Fasciclin-Like	177:190	arg1	family					222:227	the Fasciclin-Like Arabinogalactan-protein (FLA) family	173:227	the Fasciclin-Like Arabinogalactan-protein (FLA) family of extracellular glycoproteins	173:258	The predominant Fascilin 1 (FAS1)-containing proteins in plants belong to the Fasciclin-Like Arabinogalactan-protein (FLA) family of extracellular glycoproteins.
33362841	9	69	theme	reduced	1496:1502	arg1	length					1509:1514	reduced stem length	1496:1514	reduced stem length	1496:1514	Investigation of a fla16 mutant showed loss of FLA16 leads to reduced stem length and altered biomechanical properties, likely as a result of reduced levels of cellulose.
33362841	6	70	theme	B	994:994	arg1	FLA16					996:1000	the group B FLA16	984:1000	the group B FLA16 in Arabidopsis that is predominantly expressed in inflorescence tissues	984:1072	We investigated the group B FLA16 in Arabidopsis that is predominantly expressed in inflorescence tissues.
33362841	6	71	from	FLA16	996:1000	arg1	Arabidopsis					1005:1015	Arabidopsis	1005:1015	Arabidopsis	1005:1015	We investigated the group B FLA16 in Arabidopsis that is predominantly expressed in inflorescence tissues.
33362841	9	72	theme	altered	1520:1526	arg1	properties					1542:1551	altered biomechanical properties	1520:1551	altered biomechanical properties	1520:1551	Investigation of a fla16 mutant showed loss of FLA16 leads to reduced stem length and altered biomechanical properties, likely as a result of reduced levels of cellulose.
33362841	5	73	theme	function	958:965	arg1	understanding					935:947	understanding	935:947	understanding of their function	935:965	None of the group B members have been functionally characterized or their sub-cellular location resolved, limiting understanding of their function.
33362841	10	74	dep	wall	1703:1706	arg1	apoplastic					1686:1695	apoplastic	1686:1695	apoplastic	1686:1695	Immuno-labeling indicated support for FLA16 location to the plasma-membrane and (apoplastic) cell wall of interfascicular stem fiber cells.
33362841	4	75	contain	contain	753:759	arg2	domains					770:776	two FAS1 domains	761:776	two FAS1 domains	761:776	FLA group B members contain two FAS1 domains and are not predicted to be GPI-anchored.
33362841	4	75	contain	contain	753:759	arg1	members					745:751	FLA group B members	733:751	FLA group B members	733:751	FLA group B members contain two FAS1 domains and are not predicted to be GPI-anchored.
33362841	9	76	theme	mutant	1459:1464	arg1	Investigation					1434:1446	Investigation	1434:1446	Investigation of a fla16 mutant	1434:1464	Investigation of a fla16 mutant showed loss of FLA16 leads to reduced stem length and altered biomechanical properties, likely as a result of reduced levels of cellulose.
33362841	8	77	theme	cell	1330:1333	arg1	walls					1335:1339	secondary cell walls	1320:1339	secondary cell walls	1320:1339	A FLA16-YFP fusion protein driven by the endogenous putative FLA16 promoter in wild type background showed expression in cells with secondary cell walls, and FLA16 displayed characteristics of cell wall glycoproteins with moderate glycosylation.
33362841	1	78	gly	glycoproteins	246:258	arg1	glycoproteins					246:258	extracellular glycoproteins	232:258	extracellular glycoproteins	232:258	The predominant Fascilin 1 (FAS1)-containing proteins in plants belong to the Fasciclin-Like Arabinogalactan-protein (FLA) family of extracellular glycoproteins.
33362841	2	79	theme	large	381:385	arg1	AG					404:405	AG	404:405	AG	404:405	In addition to FAS1 domains, these multi-domain FLA proteins contain glycomotif regions predicted to direct addition of large arabinogalactan (AG) glycans and many contain signal sequences for addition of a glycosylphosphatidylinositol (GPI)-anchor to tether them to the plasma membrane.
33362841	2	79	theme	large	381:385	arg1	arabinogalactan					387:401	large arabinogalactan	381:401	large arabinogalactan (AG) glycans	381:414	In addition to FAS1 domains, these multi-domain FLA proteins contain glycomotif regions predicted to direct addition of large arabinogalactan (AG) glycans and many contain signal sequences for addition of a glycosylphosphatidylinositol (GPI)-anchor to tether them to the plasma membrane.
33362841	4	80	theme	group	737:741	arg1	members					745:751	FLA group B members	733:751	FLA group B members	733:751	FLA group B members contain two FAS1 domains and are not predicted to be GPI-anchored.
33362841	7	81	theme	Group	1132:1136	arg1	FLA11					1148:1152	FLA11	1148:1152	FLA11	1148:1152	FLA16 is the most highly expressed FLA in the stem after Group A members FLA11 and FLA12 that are stem specific.
33362841	7	81	theme	Group	1132:1136	arg1	members					1140:1146	Group A members FLA11 and FLA12	1132:1162	Group A members FLA11 and FLA12	1132:1162	FLA16 is the most highly expressed FLA in the stem after Group A members FLA11 and FLA12 that are stem specific.
33362841	7	81	theme	Group	1132:1136	arg1	FLA12					1158:1162	FLA12	1158:1162	FLA12	1158:1162	FLA16 is the most highly expressed FLA in the stem after Group A members FLA11 and FLA12 that are stem specific.
33362841	9	82	theme	levels	1584:1589	arg1	result					1566:1571	a result	1564:1571	a result of reduced levels of cellulose	1564:1602	Investigation of a fla16 mutant showed loss of FLA16 leads to reduced stem length and altered biomechanical properties, likely as a result of reduced levels of cellulose.
33362841	8	83	with	cells	1309:1313	arg1	walls					1335:1339	secondary cell walls	1320:1339	secondary cell walls	1320:1339	A FLA16-YFP fusion protein driven by the endogenous putative FLA16 promoter in wild type background showed expression in cells with secondary cell walls, and FLA16 displayed characteristics of cell wall glycoproteins with moderate glycosylation.
33362841	8	84	theme	cell	1381:1384	arg1	glycoproteins					1391:1403	cell wall glycoproteins	1381:1403	cell wall glycoproteins with moderate glycosylation	1381:1431	A FLA16-YFP fusion protein driven by the endogenous putative FLA16 promoter in wild type background showed expression in cells with secondary cell walls, and FLA16 displayed characteristics of cell wall glycoproteins with moderate glycosylation.
33362841	11	85	theme	secondary	1830:1838	arg1	synthesis					1850:1858	plant secondary cell wall synthesis	1824:1858	plant secondary cell wall synthesis	1824:1858	Together these results indicate FLA16, a two-FAS1 domain FLAs, plays a role in plant secondary cell wall synthesis and function.
33362841	9	86	theme	cellulose	1594:1602	arg1	levels					1584:1589	reduced levels	1576:1589	reduced levels of cellulose	1576:1602	Investigation of a fla16 mutant showed loss of FLA16 leads to reduced stem length and altered biomechanical properties, likely as a result of reduced levels of cellulose.
33362841	8	87	theme	glycoproteins	1391:1403	arg1	characteristics					1362:1376	characteristics	1362:1376	characteristics of cell wall glycoproteins with moderate glycosylation	1362:1431	A FLA16-YFP fusion protein driven by the endogenous putative FLA16 promoter in wild type background showed expression in cells with secondary cell walls, and FLA16 displayed characteristics of cell wall glycoproteins with moderate glycosylation.
33362841	8	88	theme	moderate	1410:1417	arg1	glycosylation					1419:1431	moderate glycosylation	1410:1431	moderate glycosylation	1410:1431	A FLA16-YFP fusion protein driven by the endogenous putative FLA16 promoter in wild type background showed expression in cells with secondary cell walls, and FLA16 displayed characteristics of cell wall glycoproteins with moderate glycosylation.
33362841	10	89	theme	interfascicular	1711:1725	arg1	cells					1738:1742	interfascicular stem fiber cells	1711:1742	interfascicular stem fiber cells	1711:1742	Immuno-labeling indicated support for FLA16 location to the plasma-membrane and (apoplastic) cell wall of interfascicular stem fiber cells.
33362841	1	90	theme	extracellular	232:244	arg1	glycoproteins					246:258	extracellular glycoproteins	232:258	extracellular glycoproteins	232:258	The predominant Fascilin 1 (FAS1)-containing proteins in plants belong to the Fasciclin-Like Arabinogalactan-protein (FLA) family of extracellular glycoproteins.
33932348	6	0	from	cells	726:730	arg1	nodes					773:777	mesenteric lymph nodes	756:777	mesenteric lymph nodes (MLNs)	756:784	Th17 cells in peripheral blood and mesenteric lymph nodes (MLNs) were assessed using flow cytometry.
33932348	6	0	from	cells	726:730	arg1	blood					746:750	peripheral blood	735:750	peripheral blood	735:750	Th17 cells in peripheral blood and mesenteric lymph nodes (MLNs) were assessed using flow cytometry.
33932348	6	0	from	cells	726:730	arg1	MLNs					780:783	MLNs	780:783	MLNs	780:783	Th17 cells in peripheral blood and mesenteric lymph nodes (MLNs) were assessed using flow cytometry.
33932348	11	1	theme	colitis	1375:1381	arg1	mice					1393:1396	colitis wild-type mice	1375:1396	colitis wild-type mice	1375:1396	In contrast to colitis wild-type mice, the composition of colonic microbiota was shifted in colitis TRAIL-/- mice, and was characterized by increased alpha diversity, increased TM7, deferribacteres and tenericutes, and decreased proteobacteria at the phylum level.
33932348	2	2	theme	experimental	357:368	arg1	model					384:388	experimental colitis mouse model	357:388	experimental colitis mouse model	357:388	This study investigated the influence of TRAIL deficiency on Th17 cells and colonic microbiota in experimental colitis mouse model.
33932348	7	3	theme	Th17	847:850	arg1	markers					857:863	Th17 cell markers IL-17A and ROR-γt	847:881	Th17 cell markers IL-17A and ROR-γt	847:881	The expression levels of Th17 cell markers IL-17A and ROR-γt were evaluated by quantitative real-time polymerase chain reaction.
33932348	7	3	theme	Th17	847:850	arg1	ROR-γt					876:881	ROR-γt	876:881	ROR-γt	876:881	The expression levels of Th17 cell markers IL-17A and ROR-γt were evaluated by quantitative real-time polymerase chain reaction.
33932348	7	3	theme	Th17	847:850	arg1	IL-17A					865:870	IL-17A	865:870	IL-17A	865:870	The expression levels of Th17 cell markers IL-17A and ROR-γt were evaluated by quantitative real-time polymerase chain reaction.
33932348	8	4	theme	colonic	955:961	arg1	samples					963:969	The colonic samples	951:969	The colonic samples	951:969	The colonic samples were also analyzed for microbiota profile by 16s-rDNA gene sequencing on variable V4 region.
33932348	4	5	theme	dextran	583:589	arg1	DSS					607:609	3.5% dextran sulfate sodium (DSS)	578:610	3.5% dextran sulfate sodium (DSS) for 7 consecutive days	578:633	Colitis was induced by oral administration of 3.5% dextran sulfate sodium (DSS) for 7 consecutive days.
33932348	3	6	theme	colitis	495:501	arg1	groups					524:529	wild-type colitis and TRAIL-/- colitis groups	485:529	wild-type colitis and TRAIL-/- colitis groups	485:529	METHODS Mice were randomly divided into 4 groups: wild-type, TRAIL gene knock-out (TRAIL-/-), wild-type colitis and TRAIL-/- colitis groups.
33932348	3	6	theme	colitis	495:501	arg1	groups					433:438	4 groups	431:438	4 groups	431:438	METHODS Mice were randomly divided into 4 groups: wild-type, TRAIL gene knock-out (TRAIL-/-), wild-type colitis and TRAIL-/- colitis groups.
33932348	11	7	theme	microbiota	1426:1435	arg1	composition					1403:1413	the composition	1399:1413	the composition of colonic microbiota	1399:1435	In contrast to colitis wild-type mice, the composition of colonic microbiota was shifted in colitis TRAIL-/- mice, and was characterized by increased alpha diversity, increased TM7, deferribacteres and tenericutes, and decreased proteobacteria at the phylum level.
33932348	12	8	theme	altered	1773:1779	arg1	composition					1800:1810	altered colonic microbiota composition	1773:1810	altered colonic microbiota composition	1773:1810	CONCLUSIONS These findings suggested that TRAIL deficiency not only aggravated DSS-induced colitis, but also led to enhanced Th17 cell response and altered colonic microbiota composition.
33932348	10	9	contain	had	1197:1199	arg2	proportion					1211:1220	increased proportion	1201:1220	increased proportion of Th17 cells	1201:1234	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	10	9	contain	had	1197:1199	arg1	mice					1192:1195	Colitis TRAIL-/- mice	1175:1195	Colitis TRAIL-/- mice	1175:1195	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	10	9	contain	had	1197:1199	arg2	levels					1265:1270	elevated mRNA expression levels	1240:1270	elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs	1240:1320	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	6	10	theme	lymph	767:771	arg1	nodes					773:777	mesenteric lymph nodes	756:777	mesenteric lymph nodes (MLNs)	756:784	Th17 cells in peripheral blood and mesenteric lymph nodes (MLNs) were assessed using flow cytometry.
33932348	6	10	theme	lymph	767:771	arg1	MLNs					780:783	MLNs	780:783	MLNs	780:783	Th17 cells in peripheral blood and mesenteric lymph nodes (MLNs) were assessed using flow cytometry.
33932348	2	11	theme	Th17	320:323	arg1	cells					325:329	Th17 cells	320:329	Th17 cells	320:329	This study investigated the influence of TRAIL deficiency on Th17 cells and colonic microbiota in experimental colitis mouse model.
33932348	8	12	theme	microbiota	994:1003	arg1	profile					1005:1011	microbiota profile	994:1011	microbiota profile	994:1011	The colonic samples were also analyzed for microbiota profile by 16s-rDNA gene sequencing on variable V4 region.
33932348	2	13	theme	deficiency	306:315	arg1	influence					287:295	the influence	283:295	the influence of TRAIL deficiency on Th17 cells and colonic microbiota	283:352	This study investigated the influence of TRAIL deficiency on Th17 cells and colonic microbiota in experimental colitis mouse model.
33932348	11	14	theme	TRAIL-/-	1460:1467	arg1	mice					1469:1472	colitis TRAIL-/- mice	1452:1472	colitis TRAIL-/- mice	1452:1472	In contrast to colitis wild-type mice, the composition of colonic microbiota was shifted in colitis TRAIL-/- mice, and was characterized by increased alpha diversity, increased TM7, deferribacteres and tenericutes, and decreased proteobacteria at the phylum level.
33932348	11	15	theme	colitis	1452:1458	arg1	mice					1469:1472	colitis TRAIL-/- mice	1452:1472	colitis TRAIL-/- mice	1452:1472	In contrast to colitis wild-type mice, the composition of colonic microbiota was shifted in colitis TRAIL-/- mice, and was characterized by increased alpha diversity, increased TM7, deferribacteres and tenericutes, and decreased proteobacteria at the phylum level.
33932348	4	16	theme	consecutive	618:628	arg1	days					630:633	7 consecutive days	616:633	7 consecutive days	616:633	Colitis was induced by oral administration of 3.5% dextran sulfate sodium (DSS) for 7 consecutive days.
33932348	1	17	theme	various	218:224	arg1	disorders					237:245	various autoimmune disorders	218:245	various autoimmune disorders	218:245	BACKGROUND The abnormalities of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) are implicated in various autoimmune disorders and tumors.
33932348	2	18	from	influence	287:295	arg1	microbiota					343:352	colonic microbiota	335:352	colonic microbiota	335:352	This study investigated the influence of TRAIL deficiency on Th17 cells and colonic microbiota in experimental colitis mouse model.
33932348	2	18	from	influence	287:295	arg1	cells					325:329	Th17 cells	320:329	Th17 cells	320:329	This study investigated the influence of TRAIL deficiency on Th17 cells and colonic microbiota in experimental colitis mouse model.
33932348	10	19	from	blood	1307:1311	arg1	levels					1265:1270	elevated mRNA expression levels	1240:1270	elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs	1240:1320	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	10	19	from	blood	1307:1311	arg1	proportion					1211:1220	increased proportion	1201:1220	increased proportion of Th17 cells	1201:1234	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	10	20	from	proportion	1211:1220	arg1	blood					1307:1311	peripheral blood	1296:1311	peripheral blood	1296:1311	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	10	20	from	proportion	1211:1220	arg1	MLNs					1317:1320	MLNs	1317:1320	MLNs	1317:1320	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	10	21	from	MLNs	1317:1320	arg1	levels					1265:1270	elevated mRNA expression levels	1240:1270	elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs	1240:1320	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	10	21	from	MLNs	1317:1320	arg1	proportion					1211:1220	increased proportion	1201:1220	increased proportion of Th17 cells	1201:1234	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	10	22	theme	Th17	1225:1228	arg1	cells					1230:1234	Th17 cells	1225:1234	Th17 cells	1225:1234	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	0	23	theme	Colonic	48:54	arg1	Microbiota					56:65	Colonic Microbiota	48:65	Colonic Microbiota	48:65	Influence of TRAIL Deficiency on Th17 Cells and Colonic Microbiota in Experimental Colitis Mouse Model.
33932348	1	24	theme	necrosis	142:149	arg1	TRAIL					193:197	TRAIL	193:197	TRAIL	193:197	BACKGROUND The abnormalities of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) are implicated in various autoimmune disorders and tumors.
33932348	1	24	theme	necrosis	142:149	arg1	ligand					185:190	tumor necrosis factor-related apoptosis-inducing ligand	136:190	tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)	136:198	BACKGROUND The abnormalities of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) are implicated in various autoimmune disorders and tumors.
33932348	5	25	theme	disease	663:669	arg1	severity					671:678	disease severity	663:678	disease severity	663:678	Mice were given scores for disease severity both clinically and histopathologically.
33932348	1	26	theme	apoptosis-inducing	166:183	arg1	TRAIL					193:197	TRAIL	193:197	TRAIL	193:197	BACKGROUND The abnormalities of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) are implicated in various autoimmune disorders and tumors.
33932348	1	26	theme	apoptosis-inducing	166:183	arg1	ligand					185:190	tumor necrosis factor-related apoptosis-inducing ligand	136:190	tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)	136:198	BACKGROUND The abnormalities of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) are implicated in various autoimmune disorders and tumors.
33932348	0	27	from	Influence	0:8	arg1	Cells					38:42	Th17 Cells	33:42	Th17 Cells	33:42	Influence of TRAIL Deficiency on Th17 Cells and Colonic Microbiota in Experimental Colitis Mouse Model.
33932348	0	27	from	Influence	0:8	arg1	Microbiota					56:65	Colonic Microbiota	48:65	Colonic Microbiota	48:65	Influence of TRAIL Deficiency on Th17 Cells and Colonic Microbiota in Experimental Colitis Mouse Model.
33932348	10	28	theme	mRNA	1249:1252	arg1	levels					1265:1270	elevated mRNA expression levels	1240:1270	elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs	1240:1320	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	0	29	theme	Colitis	83:89	arg1	Model					97:101	Experimental Colitis Mouse Model	70:101	Experimental Colitis Mouse Model	70:101	Influence of TRAIL Deficiency on Th17 Cells and Colonic Microbiota in Experimental Colitis Mouse Model.
33932348	8	30	theme	gene	1025:1028	arg1	sequencing					1030:1039	16s-rDNA gene sequencing	1016:1039	16s-rDNA gene sequencing on variable V4 region	1016:1061	The colonic samples were also analyzed for microbiota profile by 16s-rDNA gene sequencing on variable V4 region.
33932348	7	31	theme	chain	935:939	arg1	reaction					941:948	quantitative real-time polymerase chain reaction	901:948	quantitative real-time polymerase chain reaction	901:948	The expression levels of Th17 cell markers IL-17A and ROR-γt were evaluated by quantitative real-time polymerase chain reaction.
33932348	12	32	theme	TRAIL	1667:1671	arg1	deficiency					1673:1682	TRAIL deficiency	1667:1682	TRAIL deficiency	1667:1682	CONCLUSIONS These findings suggested that TRAIL deficiency not only aggravated DSS-induced colitis, but also led to enhanced Th17 cell response and altered colonic microbiota composition.
33932348	1	33	dep	BACKGROUND	104:113	arg1	implicated					204:213	implicated	204:213	are implicated in various autoimmune disorders and tumors	200:256	BACKGROUND The abnormalities of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) are implicated in various autoimmune disorders and tumors.
33932348	10	34	theme	ROR-γt	1286:1291	arg1	levels					1265:1270	elevated mRNA expression levels	1240:1270	elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs	1240:1320	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	10	34	theme	ROR-γt	1286:1291	arg1	proportion					1211:1220	increased proportion	1201:1220	increased proportion of Th17 cells	1201:1234	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	7	35	theme	real-time	914:922	arg1	reaction					941:948	quantitative real-time polymerase chain reaction	901:948	quantitative real-time polymerase chain reaction	901:948	The expression levels of Th17 cell markers IL-17A and ROR-γt were evaluated by quantitative real-time polymerase chain reaction.
33932348	10	36	theme	IL-17A	1275:1280	arg1	levels					1265:1270	elevated mRNA expression levels	1240:1270	elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs	1240:1320	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	10	36	theme	IL-17A	1275:1280	arg1	proportion					1211:1220	increased proportion	1201:1220	increased proportion of Th17 cells	1201:1234	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	8	37	theme	V4	1053:1054	arg1	region					1056:1061	variable V4 region	1044:1061	variable V4 region	1044:1061	The colonic samples were also analyzed for microbiota profile by 16s-rDNA gene sequencing on variable V4 region.
33932348	0	38	theme	TRAIL	13:17	arg1	Deficiency					19:28	TRAIL Deficiency	13:28	TRAIL Deficiency	13:28	Influence of TRAIL Deficiency on Th17 Cells and Colonic Microbiota in Experimental Colitis Mouse Model.
33932348	10	39	theme	peripheral	1296:1305	arg1	blood					1307:1311	peripheral blood	1296:1311	peripheral blood	1296:1311	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	4	40	theme	oral	555:558	arg1	administration					560:573	oral administration	555:573	oral administration of 3.5% dextran sulfate sodium (DSS) for 7 consecutive days	555:633	Colitis was induced by oral administration of 3.5% dextran sulfate sodium (DSS) for 7 consecutive days.
33932348	9	41	theme	TRAIL-/-	1110:1117	arg1	mice					1119:1122	TRAIL-/- mice	1110:1122	TRAIL-/- mice	1110:1122	RESULTS Compared with wild-type counterparts, TRAIL-/- mice developed more severe colitis after DSS treatment.
33932348	10	42	theme	colitis	1336:1342	arg1	mice					1354:1357	colitis wild-type mice	1336:1357	colitis wild-type mice	1336:1357	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	9	43	theme	severe	1139:1144	arg1	colitis					1146:1152	more severe colitis	1134:1152	more severe colitis	1134:1152	RESULTS Compared with wild-type counterparts, TRAIL-/- mice developed more severe colitis after DSS treatment.
33932348	2	44	theme	colonic	335:341	arg1	microbiota					343:352	colonic microbiota	335:352	colonic microbiota	335:352	This study investigated the influence of TRAIL deficiency on Th17 cells and colonic microbiota in experimental colitis mouse model.
33932348	1	45	theme	autoimmune	226:235	arg1	disorders					237:245	various autoimmune disorders	218:245	various autoimmune disorders	218:245	BACKGROUND The abnormalities of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) are implicated in various autoimmune disorders and tumors.
33932348	6	46	theme	flow	806:809	arg1	cytometry					811:819	flow cytometry	806:819	flow cytometry	806:819	Th17 cells in peripheral blood and mesenteric lymph nodes (MLNs) were assessed using flow cytometry.
33932348	4	47	theme	sulfate	591:597	arg1	DSS					607:609	3.5% dextran sulfate sodium (DSS)	578:610	3.5% dextran sulfate sodium (DSS) for 7 consecutive days	578:633	Colitis was induced by oral administration of 3.5% dextran sulfate sodium (DSS) for 7 consecutive days.
33932348	11	48	theme	wild-type	1383:1391	arg1	mice					1393:1396	colitis wild-type mice	1375:1396	colitis wild-type mice	1375:1396	In contrast to colitis wild-type mice, the composition of colonic microbiota was shifted in colitis TRAIL-/- mice, and was characterized by increased alpha diversity, increased TM7, deferribacteres and tenericutes, and decreased proteobacteria at the phylum level.
33932348	6	49	theme	Th17	721:724	arg1	cells					726:730	Th17 cells	721:730	Th17 cells in peripheral blood and mesenteric lymph nodes (MLNs)	721:784	Th17 cells in peripheral blood and mesenteric lymph nodes (MLNs) were assessed using flow cytometry.
33932348	3	50	theme	TRAIL-/-	507:514	arg1	colitis					516:522	TRAIL-/- colitis	507:522	TRAIL-/- colitis	507:522	METHODS Mice were randomly divided into 4 groups: wild-type, TRAIL gene knock-out (TRAIL-/-), wild-type colitis and TRAIL-/- colitis groups.
33932348	10	51	theme	Colitis	1175:1181	arg1	mice					1192:1195	Colitis TRAIL-/- mice	1175:1195	Colitis TRAIL-/- mice	1175:1195	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	0	52	dep	Influence	0:8	arg1	Model					97:101	Experimental Colitis Mouse Model	70:101	Experimental Colitis Mouse Model	70:101	Influence of TRAIL Deficiency on Th17 Cells and Colonic Microbiota in Experimental Colitis Mouse Model.
33932348	11	53	theme	phylum	1611:1616	arg1	level					1618:1622	the phylum level	1607:1622	the phylum level	1607:1622	In contrast to colitis wild-type mice, the composition of colonic microbiota was shifted in colitis TRAIL-/- mice, and was characterized by increased alpha diversity, increased TM7, deferribacteres and tenericutes, and decreased proteobacteria at the phylum level.
33932348	2	54	theme	colitis	370:376	arg1	model					384:388	experimental colitis mouse model	357:388	experimental colitis mouse model	357:388	This study investigated the influence of TRAIL deficiency on Th17 cells and colonic microbiota in experimental colitis mouse model.
33932348	2	55	theme	TRAIL	300:304	arg1	deficiency					306:315	TRAIL deficiency	300:315	TRAIL deficiency	300:315	This study investigated the influence of TRAIL deficiency on Th17 cells and colonic microbiota in experimental colitis mouse model.
33932348	7	56	theme	cell	852:855	arg1	markers					857:863	Th17 cell markers IL-17A and ROR-γt	847:881	Th17 cell markers IL-17A and ROR-γt	847:881	The expression levels of Th17 cell markers IL-17A and ROR-γt were evaluated by quantitative real-time polymerase chain reaction.
33932348	7	56	theme	cell	852:855	arg1	ROR-γt					876:881	ROR-γt	876:881	ROR-γt	876:881	The expression levels of Th17 cell markers IL-17A and ROR-γt were evaluated by quantitative real-time polymerase chain reaction.
33932348	7	56	theme	cell	852:855	arg1	IL-17A					865:870	IL-17A	865:870	IL-17A	865:870	The expression levels of Th17 cell markers IL-17A and ROR-γt were evaluated by quantitative real-time polymerase chain reaction.
33932348	10	57	theme	increased	1201:1209	arg1	proportion					1211:1220	increased proportion	1201:1220	increased proportion of Th17 cells	1201:1234	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	12	58	theme	colonic	1781:1787	arg1	composition					1800:1810	altered colonic microbiota composition	1773:1810	altered colonic microbiota composition	1773:1810	CONCLUSIONS These findings suggested that TRAIL deficiency not only aggravated DSS-induced colitis, but also led to enhanced Th17 cell response and altered colonic microbiota composition.
33932348	4	59	theme	%	581:581	arg1	DSS					607:609	3.5% dextran sulfate sodium (DSS)	578:610	3.5% dextran sulfate sodium (DSS) for 7 consecutive days	578:633	Colitis was induced by oral administration of 3.5% dextran sulfate sodium (DSS) for 7 consecutive days.
33932348	11	60	theme	increased	1527:1535	arg1	TM7					1537:1539	increased TM7	1527:1539	increased TM7	1527:1539	In contrast to colitis wild-type mice, the composition of colonic microbiota was shifted in colitis TRAIL-/- mice, and was characterized by increased alpha diversity, increased TM7, deferribacteres and tenericutes, and decreased proteobacteria at the phylum level.
33932348	1	61	theme	ligand	185:190	arg1	abnormalities					119:131	The abnormalities	115:131	The abnormalities of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)	115:198	BACKGROUND The abnormalities of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) are implicated in various autoimmune disorders and tumors.
33932348	12	62	theme	enhanced	1741:1748	arg1	response					1760:1767	enhanced Th17 cell response	1741:1767	enhanced Th17 cell response	1741:1767	CONCLUSIONS These findings suggested that TRAIL deficiency not only aggravated DSS-induced colitis, but also led to enhanced Th17 cell response and altered colonic microbiota composition.
33932348	7	63	theme	expression	826:835	arg1	levels					837:842	The expression levels	822:842	The expression levels of Th17 cell markers IL-17A and ROR-γt	822:881	The expression levels of Th17 cell markers IL-17A and ROR-γt were evaluated by quantitative real-time polymerase chain reaction.
33932348	8	64	from	sequencing	1030:1039	arg1	region					1056:1061	variable V4 region	1044:1061	variable V4 region	1044:1061	The colonic samples were also analyzed for microbiota profile by 16s-rDNA gene sequencing on variable V4 region.
33932348	12	65	theme	cell	1755:1758	arg1	response					1760:1767	enhanced Th17 cell response	1741:1767	enhanced Th17 cell response	1741:1767	CONCLUSIONS These findings suggested that TRAIL deficiency not only aggravated DSS-induced colitis, but also led to enhanced Th17 cell response and altered colonic microbiota composition.
33932348	7	66	dep	markers	857:863	arg1	markers					857:863	Th17 cell markers IL-17A and ROR-γt	847:881	Th17 cell markers IL-17A and ROR-γt	847:881	The expression levels of Th17 cell markers IL-17A and ROR-γt were evaluated by quantitative real-time polymerase chain reaction.
33932348	7	66	dep	markers	857:863	arg1	ROR-γt					876:881	ROR-γt	876:881	ROR-γt	876:881	The expression levels of Th17 cell markers IL-17A and ROR-γt were evaluated by quantitative real-time polymerase chain reaction.
33932348	7	66	dep	markers	857:863	arg1	IL-17A					865:870	IL-17A	865:870	IL-17A	865:870	The expression levels of Th17 cell markers IL-17A and ROR-γt were evaluated by quantitative real-time polymerase chain reaction.
33932348	6	67	theme	mesenteric	756:765	arg1	nodes					773:777	mesenteric lymph nodes	756:777	mesenteric lymph nodes (MLNs)	756:784	Th17 cells in peripheral blood and mesenteric lymph nodes (MLNs) were assessed using flow cytometry.
33932348	6	67	theme	mesenteric	756:765	arg1	MLNs					780:783	MLNs	780:783	MLNs	780:783	Th17 cells in peripheral blood and mesenteric lymph nodes (MLNs) were assessed using flow cytometry.
33932348	10	68	from	levels	1265:1270	arg1	blood					1307:1311	peripheral blood	1296:1311	peripheral blood	1296:1311	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	10	68	from	levels	1265:1270	arg1	MLNs					1317:1320	MLNs	1317:1320	MLNs	1317:1320	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	11	69	dep	mice	1393:1396	arg1	contrast					1363:1370	contrast	1363:1370	contrast	1363:1370	In contrast to colitis wild-type mice, the composition of colonic microbiota was shifted in colitis TRAIL-/- mice, and was characterized by increased alpha diversity, increased TM7, deferribacteres and tenericutes, and decreased proteobacteria at the phylum level.
33932348	10	70	from	IL-17A	1275:1280	arg1	blood					1307:1311	peripheral blood	1296:1311	peripheral blood	1296:1311	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	10	70	from	IL-17A	1275:1280	arg1	MLNs					1317:1320	MLNs	1317:1320	MLNs	1317:1320	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	10	71	from	ROR-γt	1286:1291	arg1	blood					1307:1311	peripheral blood	1296:1311	peripheral blood	1296:1311	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	10	71	from	ROR-γt	1286:1291	arg1	MLNs					1317:1320	MLNs	1317:1320	MLNs	1317:1320	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	10	72	theme	elevated	1240:1247	arg1	levels					1265:1270	elevated mRNA expression levels	1240:1270	elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs	1240:1320	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	12	73	theme	Th17	1750:1753	arg1	response					1760:1767	enhanced Th17 cell response	1741:1767	enhanced Th17 cell response	1741:1767	CONCLUSIONS These findings suggested that TRAIL deficiency not only aggravated DSS-induced colitis, but also led to enhanced Th17 cell response and altered colonic microbiota composition.
33932348	12	74	dep	CONCLUSIONS	1625:1635	arg1	suggested					1652:1660	suggested	1652:1660	suggested that TRAIL deficiency not only aggravated DSS-induced colitis, but also led to enhanced Th17 cell response and altered colonic microbiota composition	1652:1810	CONCLUSIONS These findings suggested that TRAIL deficiency not only aggravated DSS-induced colitis, but also led to enhanced Th17 cell response and altered colonic microbiota composition.
33932348	10	75	theme	cells	1230:1234	arg1	levels					1265:1270	elevated mRNA expression levels	1240:1270	elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs	1240:1320	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	10	75	theme	cells	1230:1234	arg1	proportion					1211:1220	increased proportion	1201:1220	increased proportion of Th17 cells	1201:1234	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	11	76	theme	increased	1500:1508	arg1	diversity					1516:1524	increased alpha diversity	1500:1524	increased alpha diversity	1500:1524	In contrast to colitis wild-type mice, the composition of colonic microbiota was shifted in colitis TRAIL-/- mice, and was characterized by increased alpha diversity, increased TM7, deferribacteres and tenericutes, and decreased proteobacteria at the phylum level.
33932348	1	77	theme	tumor	136:140	arg1	TRAIL					193:197	TRAIL	193:197	TRAIL	193:197	BACKGROUND The abnormalities of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) are implicated in various autoimmune disorders and tumors.
33932348	1	77	theme	tumor	136:140	arg1	ligand					185:190	tumor necrosis factor-related apoptosis-inducing ligand	136:190	tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)	136:198	BACKGROUND The abnormalities of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) are implicated in various autoimmune disorders and tumors.
33932348	11	78	theme	alpha	1510:1514	arg1	diversity					1516:1524	increased alpha diversity	1500:1524	increased alpha diversity	1500:1524	In contrast to colitis wild-type mice, the composition of colonic microbiota was shifted in colitis TRAIL-/- mice, and was characterized by increased alpha diversity, increased TM7, deferribacteres and tenericutes, and decreased proteobacteria at the phylum level.
33932348	0	79	theme	Experimental	70:81	arg1	Model					97:101	Experimental Colitis Mouse Model	70:101	Experimental Colitis Mouse Model	70:101	Influence of TRAIL Deficiency on Th17 Cells and Colonic Microbiota in Experimental Colitis Mouse Model.
33932348	8	80	theme	16s-rDNA	1016:1023	arg1	sequencing					1030:1039	16s-rDNA gene sequencing	1016:1039	16s-rDNA gene sequencing on variable V4 region	1016:1061	The colonic samples were also analyzed for microbiota profile by 16s-rDNA gene sequencing on variable V4 region.
33932348	1	81	theme	factor-related	151:164	arg1	TRAIL					193:197	TRAIL	193:197	TRAIL	193:197	BACKGROUND The abnormalities of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) are implicated in various autoimmune disorders and tumors.
33932348	1	81	theme	factor-related	151:164	arg1	ligand					185:190	tumor necrosis factor-related apoptosis-inducing ligand	136:190	tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)	136:198	BACKGROUND The abnormalities of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) are implicated in various autoimmune disorders and tumors.
33932348	10	82	theme	expression	1254:1263	arg1	levels					1265:1270	elevated mRNA expression levels	1240:1270	elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs	1240:1320	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	0	83	theme	Mouse	91:95	arg1	Model					97:101	Experimental Colitis Mouse Model	70:101	Experimental Colitis Mouse Model	70:101	Influence of TRAIL Deficiency on Th17 Cells and Colonic Microbiota in Experimental Colitis Mouse Model.
33932348	12	84	theme	DSS-induced	1704:1714	arg1	colitis					1716:1722	DSS-induced colitis	1704:1722	DSS-induced colitis	1704:1722	CONCLUSIONS These findings suggested that TRAIL deficiency not only aggravated DSS-induced colitis, but also led to enhanced Th17 cell response and altered colonic microbiota composition.
33932348	8	85	theme	variable	1044:1051	arg1	region					1056:1061	variable V4 region	1044:1061	variable V4 region	1044:1061	The colonic samples were also analyzed for microbiota profile by 16s-rDNA gene sequencing on variable V4 region.
33932348	11	86	theme	colonic	1418:1424	arg1	microbiota					1426:1435	colonic microbiota	1418:1435	colonic microbiota	1418:1435	In contrast to colitis wild-type mice, the composition of colonic microbiota was shifted in colitis TRAIL-/- mice, and was characterized by increased alpha diversity, increased TM7, deferribacteres and tenericutes, and decreased proteobacteria at the phylum level.
33932348	7	87	theme	polymerase	924:933	arg1	reaction					941:948	quantitative real-time polymerase chain reaction	901:948	quantitative real-time polymerase chain reaction	901:948	The expression levels of Th17 cell markers IL-17A and ROR-γt were evaluated by quantitative real-time polymerase chain reaction.
33932348	9	88	theme	wild-type	1086:1094	arg1	counterparts					1096:1107	wild-type counterparts	1086:1107	wild-type counterparts	1086:1107	RESULTS Compared with wild-type counterparts, TRAIL-/- mice developed more severe colitis after DSS treatment.
33932348	0	89	theme	Deficiency	19:28	arg1	Influence					0:8	Influence	0:8	Influence of TRAIL Deficiency on Th17 Cells and Colonic Microbiota	0:65	Influence of TRAIL Deficiency on Th17 Cells and Colonic Microbiota in Experimental Colitis Mouse Model.
33932348	7	90	theme	quantitative	901:912	arg1	reaction					941:948	quantitative real-time polymerase chain reaction	901:948	quantitative real-time polymerase chain reaction	901:948	The expression levels of Th17 cell markers IL-17A and ROR-γt were evaluated by quantitative real-time polymerase chain reaction.
33932348	3	91	theme	METHODS	391:397	arg1	Mice					399:402	METHODS Mice	391:402	METHODS Mice	391:402	METHODS Mice were randomly divided into 4 groups: wild-type, TRAIL gene knock-out (TRAIL-/-), wild-type colitis and TRAIL-/- colitis groups.
33932348	0	92	theme	Th17	33:36	arg1	Cells					38:42	Th17 Cells	33:42	Th17 Cells	33:42	Influence of TRAIL Deficiency on Th17 Cells and Colonic Microbiota in Experimental Colitis Mouse Model.
33932348	3	93	dep	groups	433:438	arg1	wild-type					441:449	wild-type	441:449	wild-type	441:449	METHODS Mice were randomly divided into 4 groups: wild-type, TRAIL gene knock-out (TRAIL-/-), wild-type colitis and TRAIL-/- colitis groups.
33932348	10	94	theme	wild-type	1344:1352	arg1	mice					1354:1357	colitis wild-type mice	1336:1357	colitis wild-type mice	1336:1357	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	3	95	dep	wild-type	441:449	arg1	TRAIL-/-					474:481	TRAIL-/-	474:481	TRAIL-/-	474:481	METHODS Mice were randomly divided into 4 groups: wild-type, TRAIL gene knock-out (TRAIL-/-), wild-type colitis and TRAIL-/- colitis groups.
33932348	3	95	dep	wild-type	441:449	arg1	knock-out					463:471	knock-out	463:471	knock-out	463:471	METHODS Mice were randomly divided into 4 groups: wild-type, TRAIL gene knock-out (TRAIL-/-), wild-type colitis and TRAIL-/- colitis groups.
33932348	6	96	theme	peripheral	735:744	arg1	blood					746:750	peripheral blood	735:750	peripheral blood	735:750	Th17 cells in peripheral blood and mesenteric lymph nodes (MLNs) were assessed using flow cytometry.
33932348	3	97	theme	wild-type	485:493	arg1	colitis					495:501	wild-type colitis	485:501	wild-type colitis	485:501	METHODS Mice were randomly divided into 4 groups: wild-type, TRAIL gene knock-out (TRAIL-/-), wild-type colitis and TRAIL-/- colitis groups.
33932348	4	98	theme	sodium	599:604	arg1	DSS					607:609	3.5% dextran sulfate sodium (DSS)	578:610	3.5% dextran sulfate sodium (DSS) for 7 consecutive days	578:633	Colitis was induced by oral administration of 3.5% dextran sulfate sodium (DSS) for 7 consecutive days.
33932348	2	99	theme	mouse	378:382	arg1	model					384:388	experimental colitis mouse model	357:388	experimental colitis mouse model	357:388	This study investigated the influence of TRAIL deficiency on Th17 cells and colonic microbiota in experimental colitis mouse model.
33932348	10	100	theme	TRAIL-/-	1183:1190	arg1	mice					1192:1195	Colitis TRAIL-/- mice	1175:1195	Colitis TRAIL-/- mice	1175:1195	Colitis TRAIL-/- mice had increased proportion of Th17 cells and elevated mRNA expression levels of IL-17A and ROR-γt in peripheral blood and MLNs compared with colitis wild-type mice.
33932348	12	101	theme	microbiota	1789:1798	arg1	composition					1800:1810	altered colonic microbiota composition	1773:1810	altered colonic microbiota composition	1773:1810	CONCLUSIONS These findings suggested that TRAIL deficiency not only aggravated DSS-induced colitis, but also led to enhanced Th17 cell response and altered colonic microbiota composition.
33932348	4	102	theme	DSS	607:609	arg1	administration					560:573	oral administration	555:573	oral administration of 3.5% dextran sulfate sodium (DSS) for 7 consecutive days	555:633	Colitis was induced by oral administration of 3.5% dextran sulfate sodium (DSS) for 7 consecutive days.
33932348	9	103	theme	DSS	1160:1162	arg1	treatment					1164:1172	DSS treatment	1160:1172	DSS treatment	1160:1172	RESULTS Compared with wild-type counterparts, TRAIL-/- mice developed more severe colitis after DSS treatment.
33932348	3	104	theme	colitis	516:522	arg1	groups					524:529	wild-type colitis and TRAIL-/- colitis groups	485:529	wild-type colitis and TRAIL-/- colitis groups	485:529	METHODS Mice were randomly divided into 4 groups: wild-type, TRAIL gene knock-out (TRAIL-/-), wild-type colitis and TRAIL-/- colitis groups.
33932348	3	104	theme	colitis	516:522	arg1	groups					433:438	4 groups	431:438	4 groups	431:438	METHODS Mice were randomly divided into 4 groups: wild-type, TRAIL gene knock-out (TRAIL-/-), wild-type colitis and TRAIL-/- colitis groups.
33932348	7	105	theme	markers	857:863	arg1	levels					837:842	The expression levels	822:842	The expression levels of Th17 cell markers IL-17A and ROR-γt	822:881	The expression levels of Th17 cell markers IL-17A and ROR-γt were evaluated by quantitative real-time polymerase chain reaction.
36750618	0	0	theme	adults	84:89	arg1	microbiota					70:79	intestinal microbiota	59:79	intestinal microbiota of adults and pre-frail elderly	59:111	In vitro metabolic capacity of carbohydrate degradation by intestinal microbiota of adults and pre-frail elderly.
36750618	3	1	theme	in	738:739	arg1	study					763:767	an in vivo GOS intervention study	735:767	an in vivo GOS intervention study based on the same subjects	735:794	The in vitro metabolic capacity was also compared with an in vivo GOS intervention study based on the same subjects.
36750618	4	2	from	portions	867:874	arg1	microbiota					900:909	overall microbiota and metabolite composition	892:936	microbiota	900:909	Analysis of 16S rRNA gene sequences and metabolites revealed distinct portions of variation in overall microbiota and metabolite composition during incubation being explained by individuality of the subjects and carbon source.
36750618	4	2	from	portions	867:874	arg1	composition					926:936	overall microbiota and metabolite composition	892:936	composition	926:936	Analysis of 16S rRNA gene sequences and metabolites revealed distinct portions of variation in overall microbiota and metabolite composition during incubation being explained by individuality of the subjects and carbon source.
36750618	2	3	theme	in	358:359	arg1	model					384:388	an in vitro batch incubation model	355:388	an in vitro batch incubation model	355:388	In the current study, we used an in vitro batch incubation model to compare the metabolic capacity of the faecal microbiota of adults (n = 6) versus pre-frail elderly (n = 6) to degrade various glycosidic carbohydrates, including galacto-oligosaccharides, 2'-fucosyllactose, chicory fructo-oligosaccharides and inulin, and isomalto/malto-polysaccharides.
36750618	7	4	theme	degradation	1552:1562	arg1	rates					1564:1568	decreased degradation rates	1542:1568	decreased degradation rates	1542:1568	Altogether, the carbohydrate degradation in elderly was different compared to adults, with some carbohydrates showing decreased degradation rates.
36750618	4	5	from	microbiota	900:909	arg1	portions					867:874	distinct portions	858:874	distinct portions of variation in overall microbiota and metabolite composition during incubation being explained by individuality of the subjects and carbon source	858:1021	Analysis of 16S rRNA gene sequences and metabolites revealed distinct portions of variation in overall microbiota and metabolite composition during incubation being explained by individuality of the subjects and carbon source.
36750618	4	6	theme	overall	892:898	arg1	microbiota					900:909	overall microbiota and metabolite composition	892:936	microbiota	900:909	Analysis of 16S rRNA gene sequences and metabolites revealed distinct portions of variation in overall microbiota and metabolite composition during incubation being explained by individuality of the subjects and carbon source.
36750618	5	7	theme	carbohydrate	1120:1131	arg1	degradation					1133:1143	carbohydrate degradation	1120:1143	carbohydrate degradation	1120:1143	In addition, the age group of the subjects also had significant impact on microbiota variation, carbohydrate degradation and metabolite production.
36750618	1	8	theme	compositional	254:266	arg1	characteristics					283:297	compositional and functional characteristics	254:297	compositional and functional characteristics of intestinal microbiota	254:322	Globally increased life expectancy strongly triggered interest to delay the onset of frailty, which has been associated with alterations in compositional and functional characteristics of intestinal microbiota.
36750618	4	9	theme	subjects	996:1003	arg1	individuality					975:987	individuality	975:987	individuality of the subjects	975:1003	Analysis of 16S rRNA gene sequences and metabolites revealed distinct portions of variation in overall microbiota and metabolite composition during incubation being explained by individuality of the subjects and carbon source.
36750618	4	9	theme	subjects	996:1003	arg1	source					1016:1021	carbon source	1009:1021	carbon source	1009:1021	Analysis of 16S rRNA gene sequences and metabolites revealed distinct portions of variation in overall microbiota and metabolite composition during incubation being explained by individuality of the subjects and carbon source.
36750618	7	10	theme	carbohydrate	1440:1451	arg1	different					1480:1488	different	1480:1488	different	1480:1488	Altogether, the carbohydrate degradation in elderly was different compared to adults, with some carbohydrates showing decreased degradation rates.
36750618	7	10	theme	carbohydrate	1440:1451	arg1	degradation					1453:1463	the carbohydrate degradation	1436:1463	the carbohydrate degradation in elderly	1436:1474	Altogether, the carbohydrate degradation in elderly was different compared to adults, with some carbohydrates showing decreased degradation rates.
36750618	2	11	theme	chicory	600:606	arg1	fructo-oligosaccharides					608:630	chicory fructo-oligosaccharides	600:630	chicory fructo-oligosaccharides	600:630	In the current study, we used an in vitro batch incubation model to compare the metabolic capacity of the faecal microbiota of adults (n = 6) versus pre-frail elderly (n = 6) to degrade various glycosidic carbohydrates, including galacto-oligosaccharides, 2'-fucosyllactose, chicory fructo-oligosaccharides and inulin, and isomalto/malto-polysaccharides.
36750618	5	12	theme	subjects	1058:1065	arg1	group					1045:1049	the age group	1037:1049	the age group of the subjects	1037:1065	In addition, the age group of the subjects also had significant impact on microbiota variation, carbohydrate degradation and metabolite production.
36750618	5	12	theme	subjects	1058:1065	arg1	subjects					1058:1065	the subjects	1054:1065	the subjects	1054:1065	In addition, the age group of the subjects also had significant impact on microbiota variation, carbohydrate degradation and metabolite production.
36750618	2	13	dep	in	358:359	arg1	vitro					361:365	vitro	361:365	vitro	361:365	In the current study, we used an in vitro batch incubation model to compare the metabolic capacity of the faecal microbiota of adults (n = 6) versus pre-frail elderly (n = 6) to degrade various glycosidic carbohydrates, including galacto-oligosaccharides, 2'-fucosyllactose, chicory fructo-oligosaccharides and inulin, and isomalto/malto-polysaccharides.
36750618	8	14	theme	health	1735:1740	arg1	benefits					1742:1749	associated prebiotic health benefits	1714:1749	associated prebiotic health benefits	1714:1749	Longer interventions periods may be required to enhance bifidobacterial abundance in the microbiota of pre-frail elderly and thereby to obtain associated prebiotic health benefits.
36750618	3	15	theme	intervention	750:761	arg1	study					763:767	an in vivo GOS intervention study	735:767	an in vivo GOS intervention study based on the same subjects	735:794	The in vitro metabolic capacity was also compared with an in vivo GOS intervention study based on the same subjects.
36750618	1	16	theme	functional	272:281	arg1	characteristics					283:297	compositional and functional characteristics	254:297	compositional and functional characteristics of intestinal microbiota	254:322	Globally increased life expectancy strongly triggered interest to delay the onset of frailty, which has been associated with alterations in compositional and functional characteristics of intestinal microbiota.
36750618	4	17	theme	carbon	1009:1014	arg1	source					1016:1021	carbon source	1009:1021	carbon source	1009:1021	Analysis of 16S rRNA gene sequences and metabolites revealed distinct portions of variation in overall microbiota and metabolite composition during incubation being explained by individuality of the subjects and carbon source.
36750618	4	18	from	variation	879:887	arg1	microbiota					900:909	overall microbiota and metabolite composition	892:936	microbiota	900:909	Analysis of 16S rRNA gene sequences and metabolites revealed distinct portions of variation in overall microbiota and metabolite composition during incubation being explained by individuality of the subjects and carbon source.
36750618	4	18	from	variation	879:887	arg1	composition					926:936	overall microbiota and metabolite composition	892:936	composition	926:936	Analysis of 16S rRNA gene sequences and metabolites revealed distinct portions of variation in overall microbiota and metabolite composition during incubation being explained by individuality of the subjects and carbon source.
36750618	6	19	theme	relative	1221:1228	arg1	abundance					1230:1238	the relative abundance	1217:1238	the relative abundance of Bifidobacterium in the microbiota of adults compared to that of pre-frail elderly and significantly decreased effectiveness to degrade galacto-oligosaccharides by the latter group	1217:1421	This was accompanied by elevated increase in the relative abundance of Bifidobacterium in the microbiota of adults compared to that of pre-frail elderly and significantly decreased effectiveness to degrade galacto-oligosaccharides by the latter group.
36750618	0	20	theme	elderly	105:111	arg1	microbiota					70:79	intestinal microbiota	59:79	intestinal microbiota of adults and pre-frail elderly	59:111	In vitro metabolic capacity of carbohydrate degradation by intestinal microbiota of adults and pre-frail elderly.
36750618	5	21	theme	metabolite	1149:1158	arg1	production					1160:1169	metabolite production	1149:1169	metabolite production	1149:1169	In addition, the age group of the subjects also had significant impact on microbiota variation, carbohydrate degradation and metabolite production.
36750618	8	22	theme	bifidobacterial	1627:1641	arg1	abundance					1643:1651	bifidobacterial abundance	1627:1651	bifidobacterial abundance	1627:1651	Longer interventions periods may be required to enhance bifidobacterial abundance in the microbiota of pre-frail elderly and thereby to obtain associated prebiotic health benefits.
36750618	0	23	theme	In	0:1	arg1	capacity					19:26	In vitro metabolic capacity	0:26	In vitro metabolic capacity of carbohydrate degradation by intestinal microbiota of adults and pre-frail elderly.	0:112	In vitro metabolic capacity of carbohydrate degradation by intestinal microbiota of adults and pre-frail elderly.
36750618	7	24	with	different	1480:1488	arg1	carbohydrates					1520:1532	some carbohydrates	1515:1532	some carbohydrates showing decreased degradation rates	1515:1568	Altogether, the carbohydrate degradation in elderly was different compared to adults, with some carbohydrates showing decreased degradation rates.
36750618	3	25	theme	GOS	746:748	arg1	study					763:767	an in vivo GOS intervention study	735:767	an in vivo GOS intervention study based on the same subjects	735:794	The in vitro metabolic capacity was also compared with an in vivo GOS intervention study based on the same subjects.
36750618	5	26	theme	age	1041:1043	arg1	group					1045:1049	the age group	1037:1049	the age group of the subjects	1037:1065	In addition, the age group of the subjects also had significant impact on microbiota variation, carbohydrate degradation and metabolite production.
36750618	5	26	theme	age	1041:1043	arg1	subjects					1058:1065	the subjects	1054:1065	the subjects	1054:1065	In addition, the age group of the subjects also had significant impact on microbiota variation, carbohydrate degradation and metabolite production.
36750618	2	27	theme	current	332:338	arg1	study					340:344	the current study	328:344	the current study	328:344	In the current study, we used an in vitro batch incubation model to compare the metabolic capacity of the faecal microbiota of adults (n = 6) versus pre-frail elderly (n = 6) to degrade various glycosidic carbohydrates, including galacto-oligosaccharides, 2'-fucosyllactose, chicory fructo-oligosaccharides and inulin, and isomalto/malto-polysaccharides.
36750618	6	28	from	abundance	1230:1238	arg1	microbiota					1266:1275	the microbiota	1262:1275	the microbiota of adults compared to that of pre-frail elderly and significantly decreased effectiveness to degrade galacto-oligosaccharides by the latter group	1262:1421	This was accompanied by elevated increase in the relative abundance of Bifidobacterium in the microbiota of adults compared to that of pre-frail elderly and significantly decreased effectiveness to degrade galacto-oligosaccharides by the latter group.
36750618	2	29	theme	microbiota	438:447	arg1	capacity					415:422	the metabolic capacity	401:422	the metabolic capacity of the faecal microbiota of adults (n = 6) versus pre-frail elderly (n = 6) to degrade various glycosidic carbohydrates, including galacto-oligosaccharides, 2'-fucosyllactose, chicory fructo-oligosaccharides and inulin, and isomalto/malto-polysaccharides	401:677	In the current study, we used an in vitro batch incubation model to compare the metabolic capacity of the faecal microbiota of adults (n = 6) versus pre-frail elderly (n = 6) to degrade various glycosidic carbohydrates, including galacto-oligosaccharides, 2'-fucosyllactose, chicory fructo-oligosaccharides and inulin, and isomalto/malto-polysaccharides.
36750618	4	30	theme	variation	879:887	arg1	portions					867:874	distinct portions	858:874	distinct portions of variation in overall microbiota and metabolite composition during incubation being explained by individuality of the subjects and carbon source	858:1021	Analysis of 16S rRNA gene sequences and metabolites revealed distinct portions of variation in overall microbiota and metabolite composition during incubation being explained by individuality of the subjects and carbon source.
36750618	8	31	theme	Longer	1571:1576	arg1	periods					1592:1598	Longer interventions periods	1571:1598	Longer interventions periods	1571:1598	Longer interventions periods may be required to enhance bifidobacterial abundance in the microbiota of pre-frail elderly and thereby to obtain associated prebiotic health benefits.
36750618	0	32	theme	degradation	44:54	arg1	capacity					19:26	In vitro metabolic capacity	0:26	In vitro metabolic capacity of carbohydrate degradation by intestinal microbiota of adults and pre-frail elderly.	0:112	In vitro metabolic capacity of carbohydrate degradation by intestinal microbiota of adults and pre-frail elderly.
36750618	2	33	theme	incubation	373:382	arg1	model					384:388	an in vitro batch incubation model	355:388	an in vitro batch incubation model	355:388	In the current study, we used an in vitro batch incubation model to compare the metabolic capacity of the faecal microbiota of adults (n = 6) versus pre-frail elderly (n = 6) to degrade various glycosidic carbohydrates, including galacto-oligosaccharides, 2'-fucosyllactose, chicory fructo-oligosaccharides and inulin, and isomalto/malto-polysaccharides.
36750618	2	34	theme	faecal	431:436	arg1	microbiota					438:447	the faecal microbiota	427:447	the faecal microbiota of adults (n = 6) versus pre-frail elderly (n = 6)	427:498	In the current study, we used an in vitro batch incubation model to compare the metabolic capacity of the faecal microbiota of adults (n = 6) versus pre-frail elderly (n = 6) to degrade various glycosidic carbohydrates, including galacto-oligosaccharides, 2'-fucosyllactose, chicory fructo-oligosaccharides and inulin, and isomalto/malto-polysaccharides.
36750618	3	35	theme	same	782:785	arg1	subjects					787:794	the same subjects	778:794	the same subjects	778:794	The in vitro metabolic capacity was also compared with an in vivo GOS intervention study based on the same subjects.
36750618	2	36	used	used	350:353	arg2	we					347:348	we	347:348	we	347:348	In the current study, we used an in vitro batch incubation model to compare the metabolic capacity of the faecal microbiota of adults (n = 6) versus pre-frail elderly (n = 6) to degrade various glycosidic carbohydrates, including galacto-oligosaccharides, 2'-fucosyllactose, chicory fructo-oligosaccharides and inulin, and isomalto/malto-polysaccharides.
36750618	6	37	theme	elevated	1196:1203	arg1	increase					1205:1212	elevated increase	1196:1212	elevated increase in the relative abundance of Bifidobacterium in the microbiota of adults compared to that of pre-frail elderly and significantly decreased effectiveness to degrade galacto-oligosaccharides by the latter group	1196:1421	This was accompanied by elevated increase in the relative abundance of Bifidobacterium in the microbiota of adults compared to that of pre-frail elderly and significantly decreased effectiveness to degrade galacto-oligosaccharides by the latter group.
36750618	1	38	theme	frailty	199:205	arg1	onset					190:194	the onset	186:194	the onset	186:194	Globally increased life expectancy strongly triggered interest to delay the onset of frailty, which has been associated with alterations in compositional and functional characteristics of intestinal microbiota.
36750618	0	39	theme	metabolic	9:17	arg1	capacity					19:26	In vitro metabolic capacity	0:26	In vitro metabolic capacity of carbohydrate degradation by intestinal microbiota of adults and pre-frail elderly.	0:112	In vitro metabolic capacity of carbohydrate degradation by intestinal microbiota of adults and pre-frail elderly.
36750618	1	40	theme	intestinal	302:311	arg1	microbiota					313:322	intestinal microbiota	302:322	intestinal microbiota	302:322	Globally increased life expectancy strongly triggered interest to delay the onset of frailty, which has been associated with alterations in compositional and functional characteristics of intestinal microbiota.
36750618	3	41	theme	in	684:685	arg1	capacity					703:710	The in vitro metabolic capacity	680:710	The in vitro metabolic capacity	680:710	The in vitro metabolic capacity was also compared with an in vivo GOS intervention study based on the same subjects.
36750618	6	42	from	increase	1205:1212	arg1	abundance					1230:1238	the relative abundance	1217:1238	the relative abundance of Bifidobacterium in the microbiota of adults compared to that of pre-frail elderly and significantly decreased effectiveness to degrade galacto-oligosaccharides by the latter group	1217:1421	This was accompanied by elevated increase in the relative abundance of Bifidobacterium in the microbiota of adults compared to that of pre-frail elderly and significantly decreased effectiveness to degrade galacto-oligosaccharides by the latter group.
36750618	7	43	from	degradation	1453:1463	arg1	elderly					1468:1474	elderly	1468:1474	elderly	1468:1474	Altogether, the carbohydrate degradation in elderly was different compared to adults, with some carbohydrates showing decreased degradation rates.
36750618	0	44	theme	carbohydrate	31:42	arg1	degradation					44:54	carbohydrate degradation	31:54	carbohydrate degradation by intestinal microbiota of adults and pre-frail elderly	31:111	In vitro metabolic capacity of carbohydrate degradation by intestinal microbiota of adults and pre-frail elderly.
36750618	1	45	theme	microbiota	313:322	arg1	characteristics					283:297	compositional and functional characteristics	254:297	compositional and functional characteristics of intestinal microbiota	254:322	Globally increased life expectancy strongly triggered interest to delay the onset of frailty, which has been associated with alterations in compositional and functional characteristics of intestinal microbiota.
36750618	2	46	theme	glycosidic	519:528	arg1	carbohydrates					530:542	various glycosidic carbohydrates	511:542	various glycosidic carbohydrates	511:542	In the current study, we used an in vitro batch incubation model to compare the metabolic capacity of the faecal microbiota of adults (n = 6) versus pre-frail elderly (n = 6) to degrade various glycosidic carbohydrates, including galacto-oligosaccharides, 2'-fucosyllactose, chicory fructo-oligosaccharides and inulin, and isomalto/malto-polysaccharides.
36750618	2	46	theme	glycosidic	519:528	arg1	galacto-oligosaccharides					555:578	galacto-oligosaccharides	555:578	galacto-oligosaccharides	555:578	In the current study, we used an in vitro batch incubation model to compare the metabolic capacity of the faecal microbiota of adults (n = 6) versus pre-frail elderly (n = 6) to degrade various glycosidic carbohydrates, including galacto-oligosaccharides, 2'-fucosyllactose, chicory fructo-oligosaccharides and inulin, and isomalto/malto-polysaccharides.
36750618	2	46	theme	glycosidic	519:528	arg1	2'-fucosyllactose					581:597	2'-fucosyllactose	581:597	2'-fucosyllactose	581:597	In the current study, we used an in vitro batch incubation model to compare the metabolic capacity of the faecal microbiota of adults (n = 6) versus pre-frail elderly (n = 6) to degrade various glycosidic carbohydrates, including galacto-oligosaccharides, 2'-fucosyllactose, chicory fructo-oligosaccharides and inulin, and isomalto/malto-polysaccharides.
36750618	2	46	theme	glycosidic	519:528	arg1	isomalto/malto-polysaccharides					648:677	isomalto/malto-polysaccharides	648:677	isomalto/malto-polysaccharides	648:677	In the current study, we used an in vitro batch incubation model to compare the metabolic capacity of the faecal microbiota of adults (n = 6) versus pre-frail elderly (n = 6) to degrade various glycosidic carbohydrates, including galacto-oligosaccharides, 2'-fucosyllactose, chicory fructo-oligosaccharides and inulin, and isomalto/malto-polysaccharides.
36750618	8	47	theme	interventions	1578:1590	arg1	periods					1592:1598	Longer interventions periods	1571:1598	Longer interventions periods	1571:1598	Longer interventions periods may be required to enhance bifidobacterial abundance in the microbiota of pre-frail elderly and thereby to obtain associated prebiotic health benefits.
36750618	4	48	theme	metabolites	837:847	arg1	Analysis					797:804	Analysis	797:804	Analysis of 16S rRNA gene sequences and metabolites	797:847	Analysis of 16S rRNA gene sequences and metabolites revealed distinct portions of variation in overall microbiota and metabolite composition during incubation being explained by individuality of the subjects and carbon source.
36750618	6	49	theme	Bifidobacterium	1243:1257	arg1	abundance					1230:1238	the relative abundance	1217:1238	the relative abundance of Bifidobacterium in the microbiota of adults compared to that of pre-frail elderly and significantly decreased effectiveness to degrade galacto-oligosaccharides by the latter group	1217:1421	This was accompanied by elevated increase in the relative abundance of Bifidobacterium in the microbiota of adults compared to that of pre-frail elderly and significantly decreased effectiveness to degrade galacto-oligosaccharides by the latter group.
36750618	2	50	theme	metabolic	405:413	arg1	capacity					415:422	the metabolic capacity	401:422	the metabolic capacity of the faecal microbiota of adults (n = 6) versus pre-frail elderly (n = 6) to degrade various glycosidic carbohydrates, including galacto-oligosaccharides, 2'-fucosyllactose, chicory fructo-oligosaccharides and inulin, and isomalto/malto-polysaccharides	401:677	In the current study, we used an in vitro batch incubation model to compare the metabolic capacity of the faecal microbiota of adults (n = 6) versus pre-frail elderly (n = 6) to degrade various glycosidic carbohydrates, including galacto-oligosaccharides, 2'-fucosyllactose, chicory fructo-oligosaccharides and inulin, and isomalto/malto-polysaccharides.
36750618	2	51	theme	various	511:517	arg1	carbohydrates					530:542	various glycosidic carbohydrates	511:542	various glycosidic carbohydrates	511:542	In the current study, we used an in vitro batch incubation model to compare the metabolic capacity of the faecal microbiota of adults (n = 6) versus pre-frail elderly (n = 6) to degrade various glycosidic carbohydrates, including galacto-oligosaccharides, 2'-fucosyllactose, chicory fructo-oligosaccharides and inulin, and isomalto/malto-polysaccharides.
36750618	2	51	theme	various	511:517	arg1	galacto-oligosaccharides					555:578	galacto-oligosaccharides	555:578	galacto-oligosaccharides	555:578	In the current study, we used an in vitro batch incubation model to compare the metabolic capacity of the faecal microbiota of adults (n = 6) versus pre-frail elderly (n = 6) to degrade various glycosidic carbohydrates, including galacto-oligosaccharides, 2'-fucosyllactose, chicory fructo-oligosaccharides and inulin, and isomalto/malto-polysaccharides.
36750618	2	51	theme	various	511:517	arg1	2'-fucosyllactose					581:597	2'-fucosyllactose	581:597	2'-fucosyllactose	581:597	In the current study, we used an in vitro batch incubation model to compare the metabolic capacity of the faecal microbiota of adults (n = 6) versus pre-frail elderly (n = 6) to degrade various glycosidic carbohydrates, including galacto-oligosaccharides, 2'-fucosyllactose, chicory fructo-oligosaccharides and inulin, and isomalto/malto-polysaccharides.
36750618	2	51	theme	various	511:517	arg1	isomalto/malto-polysaccharides					648:677	isomalto/malto-polysaccharides	648:677	isomalto/malto-polysaccharides	648:677	In the current study, we used an in vitro batch incubation model to compare the metabolic capacity of the faecal microbiota of adults (n = 6) versus pre-frail elderly (n = 6) to degrade various glycosidic carbohydrates, including galacto-oligosaccharides, 2'-fucosyllactose, chicory fructo-oligosaccharides and inulin, and isomalto/malto-polysaccharides.
36750618	6	52	theme	latter	1410:1415	arg1	group					1417:1421	the latter group	1406:1421	the latter group	1406:1421	This was accompanied by elevated increase in the relative abundance of Bifidobacterium in the microbiota of adults compared to that of pre-frail elderly and significantly decreased effectiveness to degrade galacto-oligosaccharides by the latter group.
36750618	7	53	theme	decreased	1542:1550	arg1	rates					1564:1568	decreased degradation rates	1542:1568	decreased degradation rates	1542:1568	Altogether, the carbohydrate degradation in elderly was different compared to adults, with some carbohydrates showing decreased degradation rates.
36750618	8	54	dep	elderly	1684:1690	arg1	the					1656:1658	the	1656:1658	the	1656:1658	Longer interventions periods may be required to enhance bifidobacterial abundance in the microbiota of pre-frail elderly and thereby to obtain associated prebiotic health benefits.
36750618	8	54	dep	elderly	1684:1690	arg1	microbiota					1660:1669	microbiota	1660:1669	microbiota	1660:1669	Longer interventions periods may be required to enhance bifidobacterial abundance in the microbiota of pre-frail elderly and thereby to obtain associated prebiotic health benefits.
36750618	5	55	theme	microbiota	1098:1107	arg1	variation					1109:1117	microbiota variation	1098:1117	microbiota variation	1098:1117	In addition, the age group of the subjects also had significant impact on microbiota variation, carbohydrate degradation and metabolite production.
36750618	4	56	theme	metabolite	915:924	arg1	composition					926:936	overall microbiota and metabolite composition	892:936	composition	926:936	Analysis of 16S rRNA gene sequences and metabolites revealed distinct portions of variation in overall microbiota and metabolite composition during incubation being explained by individuality of the subjects and carbon source.
36750618	2	57	theme	elderly	484:490	arg1	microbiota					438:447	the faecal microbiota	427:447	the faecal microbiota of adults (n = 6) versus pre-frail elderly (n = 6)	427:498	In the current study, we used an in vitro batch incubation model to compare the metabolic capacity of the faecal microbiota of adults (n = 6) versus pre-frail elderly (n = 6) to degrade various glycosidic carbohydrates, including galacto-oligosaccharides, 2'-fucosyllactose, chicory fructo-oligosaccharides and inulin, and isomalto/malto-polysaccharides.
36750618	4	58	theme	distinct	858:865	arg1	portions					867:874	distinct portions	858:874	distinct portions of variation in overall microbiota and metabolite composition during incubation being explained by individuality of the subjects and carbon source	858:1021	Analysis of 16S rRNA gene sequences and metabolites revealed distinct portions of variation in overall microbiota and metabolite composition during incubation being explained by individuality of the subjects and carbon source.
36750618	2	59	theme	adults	452:457	arg1	microbiota					438:447	the faecal microbiota	427:447	the faecal microbiota of adults (n = 6) versus pre-frail elderly (n = 6)	427:498	In the current study, we used an in vitro batch incubation model to compare the metabolic capacity of the faecal microbiota of adults (n = 6) versus pre-frail elderly (n = 6) to degrade various glycosidic carbohydrates, including galacto-oligosaccharides, 2'-fucosyllactose, chicory fructo-oligosaccharides and inulin, and isomalto/malto-polysaccharides.
36750618	1	60	from	alterations	239:249	arg1	characteristics					283:297	compositional and functional characteristics	254:297	compositional and functional characteristics of intestinal microbiota	254:322	Globally increased life expectancy strongly triggered interest to delay the onset of frailty, which has been associated with alterations in compositional and functional characteristics of intestinal microbiota.
36750618	5	61	contain	had	1072:1074	arg1	group					1045:1049	the age group	1037:1049	the age group of the subjects	1037:1065	In addition, the age group of the subjects also had significant impact on microbiota variation, carbohydrate degradation and metabolite production.
36750618	5	61	contain	had	1072:1074	arg1	subjects					1058:1065	the subjects	1054:1065	the subjects	1054:1065	In addition, the age group of the subjects also had significant impact on microbiota variation, carbohydrate degradation and metabolite production.
36750618	5	61	contain	had	1072:1074	arg2	impact					1088:1093	significant impact	1076:1093	significant impact	1076:1093	In addition, the age group of the subjects also had significant impact on microbiota variation, carbohydrate degradation and metabolite production.
36750618	5	62	theme	significant	1076:1086	arg1	impact					1088:1093	significant impact	1076:1093	significant impact	1076:1093	In addition, the age group of the subjects also had significant impact on microbiota variation, carbohydrate degradation and metabolite production.
36750618	1	63	theme	increased	123:131	arg1	expectancy					138:147	Globally increased life expectancy	114:147	Globally increased life expectancy	114:147	Globally increased life expectancy strongly triggered interest to delay the onset of frailty, which has been associated with alterations in compositional and functional characteristics of intestinal microbiota.
36750618	4	64	theme	gene	818:821	arg1	sequences					823:831	16S rRNA gene sequences	809:831	16S rRNA gene sequences	809:831	Analysis of 16S rRNA gene sequences and metabolites revealed distinct portions of variation in overall microbiota and metabolite composition during incubation being explained by individuality of the subjects and carbon source.
36750618	6	65	theme	decreased	1343:1351	arg1	effectiveness					1353:1365	significantly decreased effectiveness	1329:1365	significantly decreased effectiveness to degrade galacto-oligosaccharides by the latter group	1329:1421	This was accompanied by elevated increase in the relative abundance of Bifidobacterium in the microbiota of adults compared to that of pre-frail elderly and significantly decreased effectiveness to degrade galacto-oligosaccharides by the latter group.
36750618	4	66	theme	rRNA	813:816	arg1	sequences					823:831	16S rRNA gene sequences	809:831	16S rRNA gene sequences	809:831	Analysis of 16S rRNA gene sequences and metabolites revealed distinct portions of variation in overall microbiota and metabolite composition during incubation being explained by individuality of the subjects and carbon source.
36750618	3	67	dep	in	738:739	arg1	vivo					741:744	vivo	741:744	vivo	741:744	The in vitro metabolic capacity was also compared with an in vivo GOS intervention study based on the same subjects.
36750618	1	68	theme	life	133:136	arg1	expectancy					138:147	Globally increased life expectancy	114:147	Globally increased life expectancy	114:147	Globally increased life expectancy strongly triggered interest to delay the onset of frailty, which has been associated with alterations in compositional and functional characteristics of intestinal microbiota.
36750618	8	69	theme	associated	1714:1723	arg1	benefits					1742:1749	associated prebiotic health benefits	1714:1749	associated prebiotic health benefits	1714:1749	Longer interventions periods may be required to enhance bifidobacterial abundance in the microbiota of pre-frail elderly and thereby to obtain associated prebiotic health benefits.
36750618	3	70	theme	metabolic	693:701	arg1	capacity					703:710	The in vitro metabolic capacity	680:710	The in vitro metabolic capacity	680:710	The in vitro metabolic capacity was also compared with an in vivo GOS intervention study based on the same subjects.
36750618	4	71	theme	16S	809:811	arg1	sequences					823:831	16S rRNA gene sequences	809:831	16S rRNA gene sequences	809:831	Analysis of 16S rRNA gene sequences and metabolites revealed distinct portions of variation in overall microbiota and metabolite composition during incubation being explained by individuality of the subjects and carbon source.
36750618	4	72	from	composition	926:936	arg1	portions					867:874	distinct portions	858:874	distinct portions of variation in overall microbiota and metabolite composition during incubation being explained by individuality of the subjects and carbon source	858:1021	Analysis of 16S rRNA gene sequences and metabolites revealed distinct portions of variation in overall microbiota and metabolite composition during incubation being explained by individuality of the subjects and carbon source.
36750618	4	73	theme	sequences	823:831	arg1	Analysis					797:804	Analysis	797:804	Analysis of 16S rRNA gene sequences and metabolites	797:847	Analysis of 16S rRNA gene sequences and metabolites revealed distinct portions of variation in overall microbiota and metabolite composition during incubation being explained by individuality of the subjects and carbon source.
36750618	6	74	theme	adults	1280:1285	arg1	microbiota					1266:1275	the microbiota	1262:1275	the microbiota of adults compared to that of pre-frail elderly and significantly decreased effectiveness to degrade galacto-oligosaccharides by the latter group	1262:1421	This was accompanied by elevated increase in the relative abundance of Bifidobacterium in the microbiota of adults compared to that of pre-frail elderly and significantly decreased effectiveness to degrade galacto-oligosaccharides by the latter group.
36750618	2	75	theme	batch	367:371	arg1	model					384:388	an in vitro batch incubation model	355:388	an in vitro batch incubation model	355:388	In the current study, we used an in vitro batch incubation model to compare the metabolic capacity of the faecal microbiota of adults (n = 6) versus pre-frail elderly (n = 6) to degrade various glycosidic carbohydrates, including galacto-oligosaccharides, 2'-fucosyllactose, chicory fructo-oligosaccharides and inulin, and isomalto/malto-polysaccharides.
36750618	3	76	dep	in	684:685	arg1	vitro					687:691	vitro	687:691	vitro	687:691	The in vitro metabolic capacity was also compared with an in vivo GOS intervention study based on the same subjects.
36750618	0	77	theme	intestinal	59:68	arg1	microbiota					70:79	intestinal microbiota	59:79	intestinal microbiota of adults and pre-frail elderly	59:111	In vitro metabolic capacity of carbohydrate degradation by intestinal microbiota of adults and pre-frail elderly.
36750618	0	78	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro metabolic capacity of carbohydrate degradation by intestinal microbiota of adults and pre-frail elderly.
36750618	8	79	theme	prebiotic	1725:1733	arg1	benefits					1742:1749	associated prebiotic health benefits	1714:1749	associated prebiotic health benefits	1714:1749	Longer interventions periods may be required to enhance bifidobacterial abundance in the microbiota of pre-frail elderly and thereby to obtain associated prebiotic health benefits.
31940676	7	0	theme	adjusted	1164:1171	arg1	ratio					1178:1182	adjusted odds ratio	1164:1182	adjusted odds ratio	1164:1182	The cumulative incidence of thromboembolism was 0.5% in 2014 to 2016 compared with 0.3% in 2016 to 2017; adjusted odds ratio (OR) 0.60 (95% confidence interval [CI] 0.30-1.21).
31940676	7	0	theme	adjusted	1164:1171	arg1	[CI					1219:1221	95% confidence interval [CI] 0.30-1.21	1195:1232	95% confidence interval [CI] 0.30-1.21	1195:1232	The cumulative incidence of thromboembolism was 0.5% in 2014 to 2016 compared with 0.3% in 2016 to 2017; adjusted odds ratio (OR) 0.60 (95% confidence interval [CI] 0.30-1.21).
31940676	10	1	theme	BRIDGE	1664:1669	arg1	trial					1671:1675	the BRIDGE trial	1660:1675	the BRIDGE trial	1660:1675	The frequency of bridging with LMWH (14.8% in 2014-2016 vs. 16.6% in 2016-2017) as well as mean CHA2DS2-VASc scores of patients receiving bridging did not change after publication of the BRIDGE trial.
31940676	7	2	theme	thromboembolism	1087:1101	arg1	%					1110:1110	0.5%	1107:1110	0.5%	1107:1110	The cumulative incidence of thromboembolism was 0.5% in 2014 to 2016 compared with 0.3% in 2016 to 2017; adjusted odds ratio (OR) 0.60 (95% confidence interval [CI] 0.30-1.21).
31940676	7	2	theme	thromboembolism	1087:1101	arg1	incidence					1074:1082	The cumulative incidence	1059:1082	The cumulative incidence of thromboembolism	1059:1101	The cumulative incidence of thromboembolism was 0.5% in 2014 to 2016 compared with 0.3% in 2016 to 2017; adjusted odds ratio (OR) 0.60 (95% confidence interval [CI] 0.30-1.21).
31940676	8	3	dep	period	1357:1362	arg1	to					1349:1350	to	1349:1350	to	1349:1350	The cumulative incidence of major bleeding was 1.0% in the 2014 to 2016 period as compared with 1.3% in the 2016 to 2017 period; adjusted OR was 1.27 (95% CI 0.85-1.90).
31940676	9	4	theme	%	1460:1460	arg1	CI					1462:1463	95% CI 0.74-1.48	1458:1473	95% CI 0.74-1.48	1458:1473	The adjusted OR of the composite endpoint was 1.05 (95% CI 0.74-1.48).
31940676	9	4	theme	%	1460:1460	arg1	1.05					1452:1455	1.05	1452:1455	1.05	1452:1455	The adjusted OR of the composite endpoint was 1.05 (95% CI 0.74-1.48).
31940676	1	5	theme	trial	274:278	arg1	publication					248:258	the publication	244:258	the publication of the BRIDGE trial	244:278	BACKGROUND The benefit of periprocedural bridging with low-molecular-weight heparin (LMWH) in patients with atrial fibrillation has been contested by the publication of the BRIDGE trial.
31940676	8	6	from	%	1286:1286	arg1	period					1308:1313	the 2014 to 2016 period	1291:1313	the 2014 to 2016 period	1291:1313	The cumulative incidence of major bleeding was 1.0% in the 2014 to 2016 period as compared with 1.3% in the 2016 to 2017 period; adjusted OR was 1.27 (95% CI 0.85-1.90).
31940676	3	7	dep	METHODS	583:589	arg1	identified					594:603	identified	594:603	identified all procedures that required vitamin K antagonist interruption	594:666	METHODS We identified all procedures that required vitamin K antagonist interruption.
31940676	5	8	theme	relative	853:860	arg1	risks					862:866	relative risks	853:866	relative risks	853:866	Cumulative incidences 30 days postprocedure and relative risks of thromboembolic events, major bleeding, and mortality were calculated.
31940676	2	9	theme	Anticoagulation	542:556	arg1	Clinic					558:563	the Leiden Anticoagulation Clinic	531:563	the Leiden Anticoagulation Clinic	531:563	OBJECTIVE This article determines whether publication of the BRIDGE trial has led to less bridging procedures and better patient outcomes (i.e., a composite of thromboembolism, major bleeding, and death) in patients undergoing invasive procedures at the Leiden Anticoagulation Clinic, the Netherlands.
31940676	2	9	theme	Anticoagulation	542:556	arg1	Netherlands					570:580	the Netherlands	566:580	the Netherlands	566:580	OBJECTIVE This article determines whether publication of the BRIDGE trial has led to less bridging procedures and better patient outcomes (i.e., a composite of thromboembolism, major bleeding, and death) in patients undergoing invasive procedures at the Leiden Anticoagulation Clinic, the Netherlands.
31940676	11	10	dep	LMWH	1776:1779	arg1	complications					1821:1833	regarding bleeding complications	1802:1833	regarding bleeding complications	1802:1833	CONCLUSION We showed that despite publication of the BRIDGE trial, the frequency of bridging with LMWH and patient outcomes regarding bleeding complications did not change.
31940676	5	11	theme	Cumulative	805:814	arg1	incidences					816:825	Cumulative incidences	805:825	Cumulative incidences	805:825	Cumulative incidences 30 days postprocedure and relative risks of thromboembolic events, major bleeding, and mortality were calculated.
31940676	11	12	theme	regarding	1802:1810	arg1	complications					1821:1833	regarding bleeding complications	1802:1833	regarding bleeding complications	1802:1833	CONCLUSION We showed that despite publication of the BRIDGE trial, the frequency of bridging with LMWH and patient outcomes regarding bleeding complications did not change.
31940676	1	13	theme	periprocedural	120:133	arg1	benefit					109:115	The benefit	105:115	The benefit of periprocedural bridging with low-molecular-weight heparin (LMWH) in patients with atrial fibrillation	105:220	BACKGROUND The benefit of periprocedural bridging with low-molecular-weight heparin (LMWH) in patients with atrial fibrillation has been contested by the publication of the BRIDGE trial.
31940676	2	14	dep	OBJECTIVE	281:289	arg1	determines					304:313	determines	304:313	determines whether publication of the BRIDGE trial has led to less bridging procedures and better patient outcomes (i.e., a composite of thromboembolism, major bleeding, and death) in patients undergoing invasive procedures at the Leiden Anticoagulation Clinic, the Netherlands	304:580	OBJECTIVE This article determines whether publication of the BRIDGE trial has led to less bridging procedures and better patient outcomes (i.e., a composite of thromboembolism, major bleeding, and death) in patients undergoing invasive procedures at the Leiden Anticoagulation Clinic, the Netherlands.
31940676	10	15	theme	CHA2DS2-VASc	1573:1584	arg1	scores					1586:1591	mean CHA2DS2-VASc scores	1568:1591	LMWH (14.8% in 2014-2016 vs. 16.6% in 2016-2017) as well as mean CHA2DS2-VASc scores of patients receiving bridging	1508:1622	The frequency of bridging with LMWH (14.8% in 2014-2016 vs. 16.6% in 2016-2017) as well as mean CHA2DS2-VASc scores of patients receiving bridging did not change after publication of the BRIDGE trial.
31940676	6	16	theme	eligible	976:983	arg1	procedures					985:994	4,892 and 4,237 eligible procedures	960:994	4,892 and 4,237 eligible procedures	960:994	RESULTS A total of 4,892 and 4,237 eligible procedures were performed in 2014 to 2016 and 2016 to 2017, respectively.
31940676	0	17	theme	Cohort	80:85	arg1	Study					87:91	Observational Cohort Study	66:91	Perioperative Management in Patients Using Vitamin K Antagonists: Observational Cohort Study.	0:92	Perioperative Management in Patients Using Vitamin K Antagonists: Observational Cohort Study.
31940676	5	18	theme	events	886:891	arg1	incidences					816:825	Cumulative incidences	805:825	Cumulative incidences	805:825	Cumulative incidences 30 days postprocedure and relative risks of thromboembolic events, major bleeding, and mortality were calculated.
31940676	5	18	theme	events	886:891	arg1	risks					862:866	relative risks	853:866	relative risks	853:866	Cumulative incidences 30 days postprocedure and relative risks of thromboembolic events, major bleeding, and mortality were calculated.
31940676	7	19	dep	2016	1123:1126	arg1	to					1120:1121	to	1120:1121	to	1120:1121	The cumulative incidence of thromboembolism was 0.5% in 2014 to 2016 compared with 0.3% in 2016 to 2017; adjusted odds ratio (OR) 0.60 (95% confidence interval [CI] 0.30-1.21).
31940676	8	20	theme	adjusted	1365:1372	arg1	OR					1374:1375	adjusted OR	1365:1375	adjusted OR	1365:1375	The cumulative incidence of major bleeding was 1.0% in the 2014 to 2016 period as compared with 1.3% in the 2016 to 2017 period; adjusted OR was 1.27 (95% CI 0.85-1.90).
31940676	6	21	dep	2016	1022:1025	arg1	to					1019:1020	to	1019:1020	to	1019:1020	RESULTS A total of 4,892 and 4,237 eligible procedures were performed in 2014 to 2016 and 2016 to 2017, respectively.
31940676	2	22	theme	patient	402:408	arg1	outcomes					410:417	better patient outcomes	395:417	better patient outcomes (i.e., a composite of thromboembolism, major bleeding, and death)	395:483	OBJECTIVE This article determines whether publication of the BRIDGE trial has led to less bridging procedures and better patient outcomes (i.e., a composite of thromboembolism, major bleeding, and death) in patients undergoing invasive procedures at the Leiden Anticoagulation Clinic, the Netherlands.
31940676	8	23	from	%	1335:1335	arg1	period					1357:1362	the 2016 to 2017 period	1340:1362	the 2016 to 2017 period	1340:1362	The cumulative incidence of major bleeding was 1.0% in the 2014 to 2016 period as compared with 1.3% in the 2016 to 2017 period; adjusted OR was 1.27 (95% CI 0.85-1.90).
31940676	11	24	with	frequency	1749:1757	arg1	LMWH					1776:1779	LMWH	1776:1779	LMWH	1776:1779	CONCLUSION We showed that despite publication of the BRIDGE trial, the frequency of bridging with LMWH and patient outcomes regarding bleeding complications did not change.
31940676	11	24	with	frequency	1749:1757	arg1	outcomes					1793:1800	patient outcomes	1785:1800	patient outcomes	1785:1800	CONCLUSION We showed that despite publication of the BRIDGE trial, the frequency of bridging with LMWH and patient outcomes regarding bleeding complications did not change.
31940676	2	25	theme	thromboembolism	441:455	arg1	thromboembolism					441:455	thromboembolism	441:455	thromboembolism	441:455	OBJECTIVE This article determines whether publication of the BRIDGE trial has led to less bridging procedures and better patient outcomes (i.e., a composite of thromboembolism, major bleeding, and death) in patients undergoing invasive procedures at the Leiden Anticoagulation Clinic, the Netherlands.
31940676	2	25	theme	thromboembolism	441:455	arg1	composite					428:436	a composite	426:436	a composite of thromboembolism	426:455	OBJECTIVE This article determines whether publication of the BRIDGE trial has led to less bridging procedures and better patient outcomes (i.e., a composite of thromboembolism, major bleeding, and death) in patients undergoing invasive procedures at the Leiden Anticoagulation Clinic, the Netherlands.
31940676	2	25	theme	thromboembolism	441:455	arg1	death					478:482	death	478:482	death	478:482	OBJECTIVE This article determines whether publication of the BRIDGE trial has led to less bridging procedures and better patient outcomes (i.e., a composite of thromboembolism, major bleeding, and death) in patients undergoing invasive procedures at the Leiden Anticoagulation Clinic, the Netherlands.
31940676	7	26	dep	%	1110:1110	arg1	ratio					1178:1182	adjusted odds ratio	1164:1182	adjusted odds ratio	1164:1182	The cumulative incidence of thromboembolism was 0.5% in 2014 to 2016 compared with 0.3% in 2016 to 2017; adjusted odds ratio (OR) 0.60 (95% confidence interval [CI] 0.30-1.21).
31940676	7	26	dep	%	1110:1110	arg1	0.60					1189:1192	0.60	1189:1192	0.60	1189:1192	The cumulative incidence of thromboembolism was 0.5% in 2014 to 2016 compared with 0.3% in 2016 to 2017; adjusted odds ratio (OR) 0.60 (95% confidence interval [CI] 0.30-1.21).
31940676	7	26	dep	%	1110:1110	arg1	[CI					1219:1221	95% confidence interval [CI] 0.30-1.21	1195:1232	95% confidence interval [CI] 0.30-1.21	1195:1232	The cumulative incidence of thromboembolism was 0.5% in 2014 to 2016 compared with 0.3% in 2016 to 2017; adjusted odds ratio (OR) 0.60 (95% confidence interval [CI] 0.30-1.21).
31940676	0	27	theme	Perioperative	0:12	arg1	Management					14:23	Perioperative Management	0:23	Perioperative Management in Patients Using Vitamin K Antagonists: Observational Cohort Study.	0:92	Perioperative Management in Patients Using Vitamin K Antagonists: Observational Cohort Study.
31940676	1	28	with	periprocedural	120:133	arg1	heparin					170:176	low-molecular-weight heparin	149:176	low-molecular-weight heparin (LMWH)	149:183	BACKGROUND The benefit of periprocedural bridging with low-molecular-weight heparin (LMWH) in patients with atrial fibrillation has been contested by the publication of the BRIDGE trial.
31940676	1	28	with	periprocedural	120:133	arg1	LMWH					179:182	LMWH	179:182	LMWH	179:182	BACKGROUND The benefit of periprocedural bridging with low-molecular-weight heparin (LMWH) in patients with atrial fibrillation has been contested by the publication of the BRIDGE trial.
31940676	5	29	theme	mortality	914:922	arg1	incidences					816:825	Cumulative incidences	805:825	Cumulative incidences	805:825	Cumulative incidences 30 days postprocedure and relative risks of thromboembolic events, major bleeding, and mortality were calculated.
31940676	5	29	theme	mortality	914:922	arg1	risks					862:866	relative risks	853:866	relative risks	853:866	Cumulative incidences 30 days postprocedure and relative risks of thromboembolic events, major bleeding, and mortality were calculated.
31940676	6	30	dep	2017	1039:1042	arg1	to					1036:1037	to	1036:1037	to	1036:1037	RESULTS A total of 4,892 and 4,237 eligible procedures were performed in 2014 to 2016 and 2016 to 2017, respectively.
31940676	7	31	theme	interval	1210:1217	arg1	ratio					1178:1182	adjusted odds ratio	1164:1182	adjusted odds ratio	1164:1182	The cumulative incidence of thromboembolism was 0.5% in 2014 to 2016 compared with 0.3% in 2016 to 2017; adjusted odds ratio (OR) 0.60 (95% confidence interval [CI] 0.30-1.21).
31940676	7	31	theme	interval	1210:1217	arg1	[CI					1219:1221	95% confidence interval [CI] 0.30-1.21	1195:1232	95% confidence interval [CI] 0.30-1.21	1195:1232	The cumulative incidence of thromboembolism was 0.5% in 2014 to 2016 compared with 0.3% in 2016 to 2017; adjusted odds ratio (OR) 0.60 (95% confidence interval [CI] 0.30-1.21).
31940676	8	32	theme	%	1389:1389	arg1	CI					1391:1392	95% CI 0.85-1.90	1387:1402	95% CI 0.85-1.90	1387:1402	The cumulative incidence of major bleeding was 1.0% in the 2014 to 2016 period as compared with 1.3% in the 2016 to 2017 period; adjusted OR was 1.27 (95% CI 0.85-1.90).
31940676	8	32	theme	%	1389:1389	arg1	1.27					1381:1384	1.27	1381:1384	1.27 (95% CI 0.85-1.90)	1381:1403	The cumulative incidence of major bleeding was 1.0% in the 2014 to 2016 period as compared with 1.3% in the 2016 to 2017 period; adjusted OR was 1.27 (95% CI 0.85-1.90).
31940676	7	33	dep	2017	1158:1161	arg1	to					1155:1156	to	1155:1156	to	1155:1156	The cumulative incidence of thromboembolism was 0.5% in 2014 to 2016 compared with 0.3% in 2016 to 2017; adjusted odds ratio (OR) 0.60 (95% confidence interval [CI] 0.30-1.21).
31940676	7	34	theme	%	1197:1197	arg1	ratio					1178:1182	adjusted odds ratio	1164:1182	adjusted odds ratio	1164:1182	The cumulative incidence of thromboembolism was 0.5% in 2014 to 2016 compared with 0.3% in 2016 to 2017; adjusted odds ratio (OR) 0.60 (95% confidence interval [CI] 0.30-1.21).
31940676	7	34	theme	%	1197:1197	arg1	[CI					1219:1221	95% confidence interval [CI] 0.30-1.21	1195:1232	95% confidence interval [CI] 0.30-1.21	1195:1232	The cumulative incidence of thromboembolism was 0.5% in 2014 to 2016 compared with 0.3% in 2016 to 2017; adjusted odds ratio (OR) 0.60 (95% confidence interval [CI] 0.30-1.21).
31940676	3	35	theme	K	642:642	arg1	interruption					655:666	vitamin K antagonist interruption	634:666	vitamin K antagonist interruption	634:666	METHODS We identified all procedures that required vitamin K antagonist interruption.
31940676	0	36	theme	K	51:51	arg1	Antagonists					53:63	Vitamin K Antagonists	43:63	Vitamin K Antagonists	43:63	Perioperative Management in Patients Using Vitamin K Antagonists: Observational Cohort Study.
31940676	11	37	dep	CONCLUSION	1678:1687	arg1	showed					1692:1697	showed	1692:1697	showed that despite publication of the BRIDGE trial, the frequency of bridging with LMWH and patient outcomes regarding bleeding complications did not change	1692:1848	CONCLUSION We showed that despite publication of the BRIDGE trial, the frequency of bridging with LMWH and patient outcomes regarding bleeding complications did not change.
31940676	11	38	theme	trial	1738:1742	arg1	publication					1712:1722	publication	1712:1722	publication of the BRIDGE trial	1712:1742	CONCLUSION We showed that despite publication of the BRIDGE trial, the frequency of bridging with LMWH and patient outcomes regarding bleeding complications did not change.
31940676	5	39	dep	incidences	816:825	arg1	postprocedure					835:847	30 days postprocedure	827:847	30 days postprocedure	827:847	Cumulative incidences 30 days postprocedure and relative risks of thromboembolic events, major bleeding, and mortality were calculated.
31940676	2	40	theme	BRIDGE	342:347	arg1	trial					349:353	the BRIDGE trial	338:353	the BRIDGE trial	338:353	OBJECTIVE This article determines whether publication of the BRIDGE trial has led to less bridging procedures and better patient outcomes (i.e., a composite of thromboembolism, major bleeding, and death) in patients undergoing invasive procedures at the Leiden Anticoagulation Clinic, the Netherlands.
31940676	1	41	from	benefit	109:115	arg1	patients					188:195	patients	188:195	patients with atrial fibrillation	188:220	BACKGROUND The benefit of periprocedural bridging with low-molecular-weight heparin (LMWH) in patients with atrial fibrillation has been contested by the publication of the BRIDGE trial.
31940676	9	42	theme	endpoint	1439:1446	arg1	OR					1419:1420	The adjusted OR	1406:1420	The adjusted OR of the composite endpoint	1406:1446	The adjusted OR of the composite endpoint was 1.05 (95% CI 0.74-1.48).
31940676	9	42	theme	endpoint	1439:1446	arg1	1.05					1452:1455	1.05	1452:1455	1.05	1452:1455	The adjusted OR of the composite endpoint was 1.05 (95% CI 0.74-1.48).
31940676	2	43	theme	bridging	371:378	arg1	procedures					380:389	less bridging procedures	366:389	less bridging procedures	366:389	OBJECTIVE This article determines whether publication of the BRIDGE trial has led to less bridging procedures and better patient outcomes (i.e., a composite of thromboembolism, major bleeding, and death) in patients undergoing invasive procedures at the Leiden Anticoagulation Clinic, the Netherlands.
31940676	10	44	theme	trial	1671:1675	arg1	publication					1645:1655	publication	1645:1655	publication of the BRIDGE trial	1645:1675	The frequency of bridging with LMWH (14.8% in 2014-2016 vs. 16.6% in 2016-2017) as well as mean CHA2DS2-VASc scores of patients receiving bridging did not change after publication of the BRIDGE trial.
31940676	7	45	theme	odds	1173:1176	arg1	ratio					1178:1182	adjusted odds ratio	1164:1182	adjusted odds ratio	1164:1182	The cumulative incidence of thromboembolism was 0.5% in 2014 to 2016 compared with 0.3% in 2016 to 2017; adjusted odds ratio (OR) 0.60 (95% confidence interval [CI] 0.30-1.21).
31940676	7	45	theme	odds	1173:1176	arg1	[CI					1219:1221	95% confidence interval [CI] 0.30-1.21	1195:1232	95% confidence interval [CI] 0.30-1.21	1195:1232	The cumulative incidence of thromboembolism was 0.5% in 2014 to 2016 compared with 0.3% in 2016 to 2017; adjusted odds ratio (OR) 0.60 (95% confidence interval [CI] 0.30-1.21).
31940676	7	46	theme	cumulative	1063:1072	arg1	%					1110:1110	0.5%	1107:1110	0.5%	1107:1110	The cumulative incidence of thromboembolism was 0.5% in 2014 to 2016 compared with 0.3% in 2016 to 2017; adjusted odds ratio (OR) 0.60 (95% confidence interval [CI] 0.30-1.21).
31940676	7	46	theme	cumulative	1063:1072	arg1	incidence					1074:1082	The cumulative incidence	1059:1082	The cumulative incidence of thromboembolism	1059:1101	The cumulative incidence of thromboembolism was 0.5% in 2014 to 2016 compared with 0.3% in 2016 to 2017; adjusted odds ratio (OR) 0.60 (95% confidence interval [CI] 0.30-1.21).
31940676	11	47	theme	bridging	1762:1769	arg1	frequency					1749:1757	the frequency	1745:1757	the frequency of bridging with LMWH and patient outcomes regarding bleeding complications	1745:1833	CONCLUSION We showed that despite publication of the BRIDGE trial, the frequency of bridging with LMWH and patient outcomes regarding bleeding complications did not change.
31940676	1	48	theme	BRIDGE	267:272	arg1	trial					274:278	the BRIDGE trial	263:278	the BRIDGE trial	263:278	BACKGROUND The benefit of periprocedural bridging with low-molecular-weight heparin (LMWH) in patients with atrial fibrillation has been contested by the publication of the BRIDGE trial.
31940676	5	49	theme	days	830:833	arg1	postprocedure					835:847	30 days postprocedure	827:847	30 days postprocedure	827:847	Cumulative incidences 30 days postprocedure and relative risks of thromboembolic events, major bleeding, and mortality were calculated.
31940676	11	50	theme	patient	1785:1791	arg1	outcomes					1793:1800	patient outcomes	1785:1800	patient outcomes	1785:1800	CONCLUSION We showed that despite publication of the BRIDGE trial, the frequency of bridging with LMWH and patient outcomes regarding bleeding complications did not change.
31940676	0	51	from	Management	14:23	arg1	Patients					28:35	Patients	28:35	Patients Using Vitamin K Antagonists	28:63	Perioperative Management in Patients Using Vitamin K Antagonists: Observational Cohort Study.
31940676	10	52	from	%	1518:1518	arg1	2014-2016					1523:1531	2014-2016	1523:1531	2014-2016	1523:1531	The frequency of bridging with LMWH (14.8% in 2014-2016 vs. 16.6% in 2016-2017) as well as mean CHA2DS2-VASc scores of patients receiving bridging did not change after publication of the BRIDGE trial.
31940676	1	53	theme	atrial	202:207	arg1	fibrillation					209:220	atrial fibrillation	202:220	atrial fibrillation	202:220	BACKGROUND The benefit of periprocedural bridging with low-molecular-weight heparin (LMWH) in patients with atrial fibrillation has been contested by the publication of the BRIDGE trial.
31940676	2	54	theme	Leiden	535:540	arg1	Clinic					558:563	the Leiden Anticoagulation Clinic	531:563	the Leiden Anticoagulation Clinic	531:563	OBJECTIVE This article determines whether publication of the BRIDGE trial has led to less bridging procedures and better patient outcomes (i.e., a composite of thromboembolism, major bleeding, and death) in patients undergoing invasive procedures at the Leiden Anticoagulation Clinic, the Netherlands.
31940676	2	54	theme	Leiden	535:540	arg1	Netherlands					570:580	the Netherlands	566:580	the Netherlands	566:580	OBJECTIVE This article determines whether publication of the BRIDGE trial has led to less bridging procedures and better patient outcomes (i.e., a composite of thromboembolism, major bleeding, and death) in patients undergoing invasive procedures at the Leiden Anticoagulation Clinic, the Netherlands.
31940676	10	55	theme	bridging	1494:1501	arg1	frequency					1481:1489	The frequency	1477:1489	The frequency of bridging with LMWH (14.8% in 2014-2016 vs. 16.6% in 2016-2017) as well as mean CHA2DS2-VASc scores of patients receiving bridging	1477:1622	The frequency of bridging with LMWH (14.8% in 2014-2016 vs. 16.6% in 2016-2017) as well as mean CHA2DS2-VASc scores of patients receiving bridging did not change after publication of the BRIDGE trial.
31940676	10	56	theme	mean	1568:1571	arg1	scores					1586:1591	mean CHA2DS2-VASc scores	1568:1591	LMWH (14.8% in 2014-2016 vs. 16.6% in 2016-2017) as well as mean CHA2DS2-VASc scores of patients receiving bridging	1508:1622	The frequency of bridging with LMWH (14.8% in 2014-2016 vs. 16.6% in 2016-2017) as well as mean CHA2DS2-VASc scores of patients receiving bridging did not change after publication of the BRIDGE trial.
31940676	11	57	theme	bleeding	1812:1819	arg1	complications					1821:1833	regarding bleeding complications	1802:1833	regarding bleeding complications	1802:1833	CONCLUSION We showed that despite publication of the BRIDGE trial, the frequency of bridging with LMWH and patient outcomes regarding bleeding complications did not change.
31940676	7	58	from	%	1145:1145	arg1	2017					1158:1161	2017	1158:1161	2017	1158:1161	The cumulative incidence of thromboembolism was 0.5% in 2014 to 2016 compared with 0.3% in 2016 to 2017; adjusted odds ratio (OR) 0.60 (95% confidence interval [CI] 0.30-1.21).
31940676	8	59	dep	period	1308:1313	arg1	to					1300:1301	to	1300:1301	to	1300:1301	The cumulative incidence of major bleeding was 1.0% in the 2014 to 2016 period as compared with 1.3% in the 2016 to 2017 period; adjusted OR was 1.27 (95% CI 0.85-1.90).
31940676	6	60	dep	RESULTS	941:947	arg1	total					951:955	A total	949:955	RESULTS A total of 4,892 and 4,237 eligible procedures	941:994	RESULTS A total of 4,892 and 4,237 eligible procedures were performed in 2014 to 2016 and 2016 to 2017, respectively.
31940676	4	61	theme	trial	775:779	arg1	2014-2016					713:721	2014-2016	713:721	2014-2016; 22 months	713:732	Procedures were divided in a period before (2014-2016; 22 months) and after the publication of the BRIDGE trial (2016-2017; 22 months).
31940676	4	61	theme	trial	775:779	arg1	publication					749:759	the publication	745:759	the publication of the BRIDGE trial (2016-2017; 22 months)	745:802	Procedures were divided in a period before (2014-2016; 22 months) and after the publication of the BRIDGE trial (2016-2017; 22 months).
31940676	10	62	theme	patients	1596:1603	arg1	scores					1586:1591	mean CHA2DS2-VASc scores	1568:1591	LMWH (14.8% in 2014-2016 vs. 16.6% in 2016-2017) as well as mean CHA2DS2-VASc scores of patients receiving bridging	1508:1622	The frequency of bridging with LMWH (14.8% in 2014-2016 vs. 16.6% in 2016-2017) as well as mean CHA2DS2-VASc scores of patients receiving bridging did not change after publication of the BRIDGE trial.
31940676	10	62	theme	patients	1596:1603	arg1	LMWH					1508:1511	LMWH	1508:1511	LMWH (14.8% in 2014-2016 vs. 16.6% in 2016-2017) as well as mean CHA2DS2-VASc scores of patients receiving bridging	1508:1622	The frequency of bridging with LMWH (14.8% in 2014-2016 vs. 16.6% in 2016-2017) as well as mean CHA2DS2-VASc scores of patients receiving bridging did not change after publication of the BRIDGE trial.
31940676	5	63	theme	bleeding	900:907	arg1	incidences					816:825	Cumulative incidences	805:825	Cumulative incidences	805:825	Cumulative incidences 30 days postprocedure and relative risks of thromboembolic events, major bleeding, and mortality were calculated.
31940676	5	63	theme	bleeding	900:907	arg1	risks					862:866	relative risks	853:866	relative risks	853:866	Cumulative incidences 30 days postprocedure and relative risks of thromboembolic events, major bleeding, and mortality were calculated.
31940676	0	64	theme	Observational	66:78	arg1	Study					87:91	Observational Cohort Study	66:91	Perioperative Management in Patients Using Vitamin K Antagonists: Observational Cohort Study.	0:92	Perioperative Management in Patients Using Vitamin K Antagonists: Observational Cohort Study.
31940676	11	65	theme	BRIDGE	1731:1736	arg1	trial					1738:1742	the BRIDGE trial	1727:1742	the BRIDGE trial	1727:1742	CONCLUSION We showed that despite publication of the BRIDGE trial, the frequency of bridging with LMWH and patient outcomes regarding bleeding complications did not change.
31940676	1	66	theme	low-molecular-weight	149:168	arg1	heparin					170:176	low-molecular-weight heparin	149:176	low-molecular-weight heparin (LMWH)	149:183	BACKGROUND The benefit of periprocedural bridging with low-molecular-weight heparin (LMWH) in patients with atrial fibrillation has been contested by the publication of the BRIDGE trial.
31940676	1	66	theme	low-molecular-weight	149:168	arg1	LMWH					179:182	LMWH	179:182	LMWH	179:182	BACKGROUND The benefit of periprocedural bridging with low-molecular-weight heparin (LMWH) in patients with atrial fibrillation has been contested by the publication of the BRIDGE trial.
31940676	6	67	theme	procedures	985:994	arg1	total					951:955	A total	949:955	RESULTS A total of 4,892 and 4,237 eligible procedures	941:994	RESULTS A total of 4,892 and 4,237 eligible procedures were performed in 2014 to 2016 and 2016 to 2017, respectively.
31940676	5	68	theme	thromboembolic	871:884	arg1	events					886:891	thromboembolic events	871:891	thromboembolic events	871:891	Cumulative incidences 30 days postprocedure and relative risks of thromboembolic events, major bleeding, and mortality were calculated.
31940676	10	69	from	%	1541:1541	arg1	2016-2017					1546:1554	2016-2017	1546:1554	2016-2017	1546:1554	The frequency of bridging with LMWH (14.8% in 2014-2016 vs. 16.6% in 2016-2017) as well as mean CHA2DS2-VASc scores of patients receiving bridging did not change after publication of the BRIDGE trial.
31940676	7	70	from	%	1110:1110	arg1	2016					1123:1126	2016	1123:1126	2016	1123:1126	The cumulative incidence of thromboembolism was 0.5% in 2014 to 2016 compared with 0.3% in 2016 to 2017; adjusted odds ratio (OR) 0.60 (95% confidence interval [CI] 0.30-1.21).
31940676	1	71	dep	BACKGROUND	94:103	arg1	contested					231:239	contested	231:239	has been contested by the publication of the BRIDGE trial	222:278	BACKGROUND The benefit of periprocedural bridging with low-molecular-weight heparin (LMWH) in patients with atrial fibrillation has been contested by the publication of the BRIDGE trial.
31940676	2	72	theme	invasive	508:515	arg1	procedures					517:526	invasive procedures	508:526	invasive procedures	508:526	OBJECTIVE This article determines whether publication of the BRIDGE trial has led to less bridging procedures and better patient outcomes (i.e., a composite of thromboembolism, major bleeding, and death) in patients undergoing invasive procedures at the Leiden Anticoagulation Clinic, the Netherlands.
31940676	10	73	dep	LMWH	1508:1511	arg1	%					1541:1541	16.6%	1537:1541	16.6% in 2016-2017	1537:1554	The frequency of bridging with LMWH (14.8% in 2014-2016 vs. 16.6% in 2016-2017) as well as mean CHA2DS2-VASc scores of patients receiving bridging did not change after publication of the BRIDGE trial.
31940676	10	73	dep	LMWH	1508:1511	arg1	%					1518:1518	14.8%	1514:1518	14.8% in 2014-2016	1514:1531	The frequency of bridging with LMWH (14.8% in 2014-2016 vs. 16.6% in 2016-2017) as well as mean CHA2DS2-VASc scores of patients receiving bridging did not change after publication of the BRIDGE trial.
31940676	2	74	dep	composite	428:436	arg1	i.e.					420:423	i.e.	420:423	i.e.	420:423	OBJECTIVE This article determines whether publication of the BRIDGE trial has led to less bridging procedures and better patient outcomes (i.e., a composite of thromboembolism, major bleeding, and death) in patients undergoing invasive procedures at the Leiden Anticoagulation Clinic, the Netherlands.
31940676	8	75	theme	cumulative	1240:1249	arg1	incidence					1251:1259	The cumulative incidence	1236:1259	The cumulative incidence of major bleeding	1236:1277	The cumulative incidence of major bleeding was 1.0% in the 2014 to 2016 period as compared with 1.3% in the 2016 to 2017 period; adjusted OR was 1.27 (95% CI 0.85-1.90).
31940676	8	75	theme	cumulative	1240:1249	arg1	%					1286:1286	1.0%	1283:1286	1.0%	1283:1286	The cumulative incidence of major bleeding was 1.0% in the 2014 to 2016 period as compared with 1.3% in the 2016 to 2017 period; adjusted OR was 1.27 (95% CI 0.85-1.90).
31940676	2	76	theme	better	395:400	arg1	outcomes					410:417	better patient outcomes	395:417	better patient outcomes (i.e., a composite of thromboembolism, major bleeding, and death)	395:483	OBJECTIVE This article determines whether publication of the BRIDGE trial has led to less bridging procedures and better patient outcomes (i.e., a composite of thromboembolism, major bleeding, and death) in patients undergoing invasive procedures at the Leiden Anticoagulation Clinic, the Netherlands.
31940676	4	77	dep	2014-2016	713:721	arg1	months					727:732	22 months	724:732	2014-2016; 22 months	713:732	Procedures were divided in a period before (2014-2016; 22 months) and after the publication of the BRIDGE trial (2016-2017; 22 months).
31940676	7	78	theme	confidence	1199:1208	arg1	ratio					1178:1182	adjusted odds ratio	1164:1182	adjusted odds ratio	1164:1182	The cumulative incidence of thromboembolism was 0.5% in 2014 to 2016 compared with 0.3% in 2016 to 2017; adjusted odds ratio (OR) 0.60 (95% confidence interval [CI] 0.30-1.21).
31940676	7	78	theme	confidence	1199:1208	arg1	[CI					1219:1221	95% confidence interval [CI] 0.30-1.21	1195:1232	95% confidence interval [CI] 0.30-1.21	1195:1232	The cumulative incidence of thromboembolism was 0.5% in 2014 to 2016 compared with 0.3% in 2016 to 2017; adjusted odds ratio (OR) 0.60 (95% confidence interval [CI] 0.30-1.21).
31940676	2	79	dep	bridging	371:378	arg1	less					366:369	less	366:369	less	366:369	OBJECTIVE This article determines whether publication of the BRIDGE trial has led to less bridging procedures and better patient outcomes (i.e., a composite of thromboembolism, major bleeding, and death) in patients undergoing invasive procedures at the Leiden Anticoagulation Clinic, the Netherlands.
31940676	3	80	theme	antagonist	644:653	arg1	interruption					655:666	vitamin K antagonist interruption	634:666	vitamin K antagonist interruption	634:666	METHODS We identified all procedures that required vitamin K antagonist interruption.
31940676	8	81	theme	bleeding	1270:1277	arg1	incidence					1251:1259	The cumulative incidence	1236:1259	The cumulative incidence of major bleeding	1236:1277	The cumulative incidence of major bleeding was 1.0% in the 2014 to 2016 period as compared with 1.3% in the 2016 to 2017 period; adjusted OR was 1.27 (95% CI 0.85-1.90).
31940676	8	81	theme	bleeding	1270:1277	arg1	%					1286:1286	1.0%	1283:1286	1.0%	1283:1286	The cumulative incidence of major bleeding was 1.0% in the 2014 to 2016 period as compared with 1.3% in the 2016 to 2017 period; adjusted OR was 1.27 (95% CI 0.85-1.90).
31940676	0	82	theme	Vitamin	43:49	arg1	Antagonists					53:63	Vitamin K Antagonists	43:63	Vitamin K Antagonists	43:63	Perioperative Management in Patients Using Vitamin K Antagonists: Observational Cohort Study.
31940676	9	83	theme	adjusted	1410:1417	arg1	OR					1419:1420	The adjusted OR	1406:1420	The adjusted OR of the composite endpoint	1406:1446	The adjusted OR of the composite endpoint was 1.05 (95% CI 0.74-1.48).
31940676	9	83	theme	adjusted	1410:1417	arg1	1.05					1452:1455	1.05	1452:1455	1.05	1452:1455	The adjusted OR of the composite endpoint was 1.05 (95% CI 0.74-1.48).
31940676	3	84	theme	vitamin	634:640	arg1	interruption					655:666	vitamin K antagonist interruption	634:666	vitamin K antagonist interruption	634:666	METHODS We identified all procedures that required vitamin K antagonist interruption.
31940676	1	85	with	patients	188:195	arg1	fibrillation					209:220	atrial fibrillation	202:220	atrial fibrillation	202:220	BACKGROUND The benefit of periprocedural bridging with low-molecular-weight heparin (LMWH) in patients with atrial fibrillation has been contested by the publication of the BRIDGE trial.
31940676	2	86	from	procedures	380:389	arg1	patients					488:495	patients	488:495	patients undergoing invasive procedures at the Leiden Anticoagulation Clinic, the Netherlands	488:580	OBJECTIVE This article determines whether publication of the BRIDGE trial has led to less bridging procedures and better patient outcomes (i.e., a composite of thromboembolism, major bleeding, and death) in patients undergoing invasive procedures at the Leiden Anticoagulation Clinic, the Netherlands.
31940676	2	87	theme	trial	349:353	arg1	publication					323:333	publication	323:333	publication of the BRIDGE trial	323:353	OBJECTIVE This article determines whether publication of the BRIDGE trial has led to less bridging procedures and better patient outcomes (i.e., a composite of thromboembolism, major bleeding, and death) in patients undergoing invasive procedures at the Leiden Anticoagulation Clinic, the Netherlands.
31940676	9	88	theme	composite	1429:1437	arg1	endpoint					1439:1446	the composite endpoint	1425:1446	the composite endpoint	1425:1446	The adjusted OR of the composite endpoint was 1.05 (95% CI 0.74-1.48).
31940676	4	89	theme	BRIDGE	768:773	arg1	months					796:801	2016-2017; 22 months	782:801	2016-2017; 22 months	782:801	Procedures were divided in a period before (2014-2016; 22 months) and after the publication of the BRIDGE trial (2016-2017; 22 months).
31940676	4	89	theme	BRIDGE	768:773	arg1	trial					775:779	the BRIDGE trial	764:779	the BRIDGE trial (2016-2017; 22 months)	764:802	Procedures were divided in a period before (2014-2016; 22 months) and after the publication of the BRIDGE trial (2016-2017; 22 months).
31940676	0	90	dep	Management	14:23	arg1	Study					87:91	Observational Cohort Study	66:91	Perioperative Management in Patients Using Vitamin K Antagonists: Observational Cohort Study.	0:92	Perioperative Management in Patients Using Vitamin K Antagonists: Observational Cohort Study.
31940676	2	91	from	outcomes	410:417	arg1	patients					488:495	patients	488:495	patients undergoing invasive procedures at the Leiden Anticoagulation Clinic, the Netherlands	488:580	OBJECTIVE This article determines whether publication of the BRIDGE trial has led to less bridging procedures and better patient outcomes (i.e., a composite of thromboembolism, major bleeding, and death) in patients undergoing invasive procedures at the Leiden Anticoagulation Clinic, the Netherlands.
32752259	0	0	theme	Cell	70:73	arg1	Lines					75:79	Colorectal Cancer Cell Lines	52:79	Colorectal Cancer Cell Lines	52:79	N-Glycoproteins Have a Major Role in MGL Binding to Colorectal Cancer Cell Lines: Associations with Overall Proteome Diversity.
32752259	5	1	gly	glycoproteins	833:845	arg1	glycoproteins					833:845	most CRC glycoproteins	824:845	most CRC glycoproteins recognized by MGL	824:863	However, we failed to detect the presence of O-linked Tn and STn glycans on most CRC glycoproteins recognized by MGL.
32752259	3	2	theme	STn	463:465	arg1	antigens					467:474	STn antigens	463:474	STn antigens	463:474	Cancer-associated aberrant glycosylation, especially the Tn and STn antigens, can be detected using the macrophage galactose-type C-type lectin (MGL/CLEC10A/CD301), which has been shown to be a promising tool for CRC prognosis.
32752259	3	2	theme	STn	463:465	arg1	glycosylation					426:438	Cancer-associated aberrant glycosylation	399:438	Cancer-associated aberrant glycosylation	399:438	Cancer-associated aberrant glycosylation, especially the Tn and STn antigens, can be detected using the macrophage galactose-type C-type lectin (MGL/CLEC10A/CD301), which has been shown to be a promising tool for CRC prognosis.
32752259	6	3	theme	O-linked	924:931	arg1	glycans					933:939	N-linked and O-linked glycans	911:939	N-linked and O-linked glycans carried by these proteins for the binding to MGL	911:988	We therefore investigated here the impact of N-linked and O-linked glycans carried by these proteins for the binding to MGL.
32752259	0	4	theme	Cancer	63:68	arg1	Lines					75:79	Colorectal Cancer Cell Lines	52:79	Colorectal Cancer Cell Lines	52:79	N-Glycoproteins Have a Major Role in MGL Binding to Colorectal Cancer Cell Lines: Associations with Overall Proteome Diversity.
32752259	7	5	from	differences	1060:1070	arg1	proteins					1075:1082	proteins	1075:1082	proteins involved in glycosylation in these cells	1075:1123	In addition, we performed quantitative proteomics to study the major differences in proteins involved in glycosylation in these cells.
32752259	5	6	theme	most	824:827	arg1	glycoproteins					833:845	most CRC glycoproteins	824:845	most CRC glycoproteins recognized by MGL	824:863	However, we failed to detect the presence of O-linked Tn and STn glycans on most CRC glycoproteins recognized by MGL.
32752259	9	7	theme	high-MGL-binding	1334:1349	arg1	phenotype					1351:1359	a high-MGL-binding phenotype	1332:1359	a high-MGL-binding phenotype	1332:1359	Finally, we highlighted both common and cell-specific processes associated with a high-MGL-binding phenotype, such as differential levels of enzymes involved in protein glycosylation, and a transcriptional factor (CDX-2) involved in their regulation.
32752259	3	8	theme	aberrant	417:424	arg1	Tn					456:457	Tn	456:457	Tn	456:457	Cancer-associated aberrant glycosylation, especially the Tn and STn antigens, can be detected using the macrophage galactose-type C-type lectin (MGL/CLEC10A/CD301), which has been shown to be a promising tool for CRC prognosis.
32752259	3	8	theme	aberrant	417:424	arg1	antigens					467:474	STn antigens	463:474	STn antigens	463:474	Cancer-associated aberrant glycosylation, especially the Tn and STn antigens, can be detected using the macrophage galactose-type C-type lectin (MGL/CLEC10A/CD301), which has been shown to be a promising tool for CRC prognosis.
32752259	3	8	theme	aberrant	417:424	arg1	glycosylation					426:438	Cancer-associated aberrant glycosylation	399:438	Cancer-associated aberrant glycosylation	399:438	Cancer-associated aberrant glycosylation, especially the Tn and STn antigens, can be detected using the macrophage galactose-type C-type lectin (MGL/CLEC10A/CD301), which has been shown to be a promising tool for CRC prognosis.
32752259	9	9	theme	transcriptional	1442:1456	arg1	CDX-2					1466:1470	CDX-2	1466:1470	CDX-2	1466:1470	Finally, we highlighted both common and cell-specific processes associated with a high-MGL-binding phenotype, such as differential levels of enzymes involved in protein glycosylation, and a transcriptional factor (CDX-2) involved in their regulation.
32752259	9	9	theme	transcriptional	1442:1456	arg1	factor					1458:1463	a transcriptional factor	1440:1463	a transcriptional factor (CDX-2) involved in their regulation	1440:1500	Finally, we highlighted both common and cell-specific processes associated with a high-MGL-binding phenotype, such as differential levels of enzymes involved in protein glycosylation, and a transcriptional factor (CDX-2) involved in their regulation.
32752259	7	10	theme	quantitative	1017:1028	arg1	proteomics					1030:1039	quantitative proteomics	1017:1039	quantitative proteomics	1017:1039	In addition, we performed quantitative proteomics to study the major differences in proteins involved in glycosylation in these cells.
32752259	4	11	theme	cells	718:722	arg1	HT29					742:745	HT29	742:745	HT29	742:745	We had recently identified the major MGL-binding glycoproteins in two high-MGL-binding CRC cells lines, HCT116 and HT29.
32752259	4	11	theme	cells	718:722	arg1	HCT116					731:736	HCT116	731:736	HCT116	731:736	We had recently identified the major MGL-binding glycoproteins in two high-MGL-binding CRC cells lines, HCT116 and HT29.
32752259	4	11	theme	cells	718:722	arg1	lines					724:728	two high-MGL-binding CRC cells lines	693:728	two high-MGL-binding CRC cells lines	693:728	We had recently identified the major MGL-binding glycoproteins in two high-MGL-binding CRC cells lines, HCT116 and HT29.
32752259	1	12	theme	high	223:226	arg1	proportion					228:237	a high proportion	221:237	a high proportion of patients diagnosed at advanced stages of the disease	221:293	Colorectal cancer (CRC) is the second-leading cause of cancer death worldwide due in part to a high proportion of patients diagnosed at advanced stages of the disease.
32752259	6	13	link	N-linked	911:918	arg1	glycans					933:939	N-linked and O-linked glycans	911:939	N-linked and O-linked glycans carried by these proteins for the binding to MGL	911:988	We therefore investigated here the impact of N-linked and O-linked glycans carried by these proteins for the binding to MGL.
32752259	4	14	theme	CRC	714:716	arg1	HT29					742:745	HT29	742:745	HT29	742:745	We had recently identified the major MGL-binding glycoproteins in two high-MGL-binding CRC cells lines, HCT116 and HT29.
32752259	4	14	theme	CRC	714:716	arg1	HCT116					731:736	HCT116	731:736	HCT116	731:736	We had recently identified the major MGL-binding glycoproteins in two high-MGL-binding CRC cells lines, HCT116 and HT29.
32752259	4	14	theme	CRC	714:716	arg1	lines					724:728	two high-MGL-binding CRC cells lines	693:728	two high-MGL-binding CRC cells lines	693:728	We had recently identified the major MGL-binding glycoproteins in two high-MGL-binding CRC cells lines, HCT116 and HT29.
32752259	8	15	theme	MGL	1221:1223	arg1	binding					1225:1231	MGL binding	1221:1231	MGL binding to CRC cell lines	1221:1249	Our results showed that N-glycans have a significant, previously underestimated, importance in MGL binding to CRC cell lines.
32752259	9	16	theme	protein	1413:1419	arg1	glycosylation					1421:1433	protein glycosylation	1413:1433	protein glycosylation	1413:1433	Finally, we highlighted both common and cell-specific processes associated with a high-MGL-binding phenotype, such as differential levels of enzymes involved in protein glycosylation, and a transcriptional factor (CDX-2) involved in their regulation.
32752259	8	17	contain	have	1160:1163	arg2	importance					1207:1216	a significant, previously underestimated, importance	1165:1216	importance	1207:1216	Our results showed that N-glycans have a significant, previously underestimated, importance in MGL binding to CRC cell lines.
32752259	8	17	contain	have	1160:1163	arg1	N-glycans					1150:1158	N-glycans	1150:1158	N-glycans	1150:1158	Our results showed that N-glycans have a significant, previously underestimated, importance in MGL binding to CRC cell lines.
32752259	3	18	dep	Tn	456:457	arg1	the					452:454	the	452:454	the	452:454	Cancer-associated aberrant glycosylation, especially the Tn and STn antigens, can be detected using the macrophage galactose-type C-type lectin (MGL/CLEC10A/CD301), which has been shown to be a promising tool for CRC prognosis.
32752259	8	19	theme	significant	1167:1177	arg1	importance					1207:1216	a significant, previously underestimated, importance	1165:1216	importance	1207:1216	Our results showed that N-glycans have a significant, previously underestimated, importance in MGL binding to CRC cell lines.
32752259	7	20	theme	major	1054:1058	arg1	differences					1060:1070	the major differences	1050:1070	the major differences in proteins involved in glycosylation in these cells	1050:1123	In addition, we performed quantitative proteomics to study the major differences in proteins involved in glycosylation in these cells.
32752259	5	21	theme	O-linked	793:800	arg1	Tn					802:803	O-linked Tn	793:803	O-linked Tn	793:803	However, we failed to detect the presence of O-linked Tn and STn glycans on most CRC glycoproteins recognized by MGL.
32752259	3	22	theme	promising	593:601	arg1	tool					603:606	a promising tool	591:606	a promising tool for CRC prognosis	591:624	Cancer-associated aberrant glycosylation, especially the Tn and STn antigens, can be detected using the macrophage galactose-type C-type lectin (MGL/CLEC10A/CD301), which has been shown to be a promising tool for CRC prognosis.
32752259	3	22	theme	promising	593:601	arg1	lectin					536:541	the macrophage galactose-type C-type lectin	499:541	the macrophage galactose-type C-type lectin (MGL/CLEC10A/CD301)	499:561	Cancer-associated aberrant glycosylation, especially the Tn and STn antigens, can be detected using the macrophage galactose-type C-type lectin (MGL/CLEC10A/CD301), which has been shown to be a promising tool for CRC prognosis.
32752259	0	23	with	Associations	82:93	arg1	Diversity					117:125	Overall Proteome Diversity	100:125	Overall Proteome Diversity	100:125	N-Glycoproteins Have a Major Role in MGL Binding to Colorectal Cancer Cell Lines: Associations with Overall Proteome Diversity.
32752259	3	24	theme	C-type	529:534	arg1	MGL/CLEC10A/CD301					544:560	MGL/CLEC10A/CD301	544:560	MGL/CLEC10A/CD301	544:560	Cancer-associated aberrant glycosylation, especially the Tn and STn antigens, can be detected using the macrophage galactose-type C-type lectin (MGL/CLEC10A/CD301), which has been shown to be a promising tool for CRC prognosis.
32752259	3	24	theme	C-type	529:534	arg1	tool					603:606	a promising tool	591:606	a promising tool for CRC prognosis	591:624	Cancer-associated aberrant glycosylation, especially the Tn and STn antigens, can be detected using the macrophage galactose-type C-type lectin (MGL/CLEC10A/CD301), which has been shown to be a promising tool for CRC prognosis.
32752259	3	24	theme	C-type	529:534	arg1	lectin					536:541	the macrophage galactose-type C-type lectin	499:541	the macrophage galactose-type C-type lectin (MGL/CLEC10A/CD301)	499:561	Cancer-associated aberrant glycosylation, especially the Tn and STn antigens, can be detected using the macrophage galactose-type C-type lectin (MGL/CLEC10A/CD301), which has been shown to be a promising tool for CRC prognosis.
32752259	5	25	theme	Tn	802:803	arg1	presence					781:788	the presence	777:788	the presence of O-linked Tn and STn glycans on most CRC glycoproteins recognized by MGL	777:863	However, we failed to detect the presence of O-linked Tn and STn glycans on most CRC glycoproteins recognized by MGL.
32752259	5	26	link	O-linked	793:800	arg1	Tn					802:803	O-linked Tn	793:803	O-linked Tn	793:803	However, we failed to detect the presence of O-linked Tn and STn glycans on most CRC glycoproteins recognized by MGL.
32752259	5	27	theme	glycans	813:819	arg1	presence					781:788	the presence	777:788	the presence of O-linked Tn and STn glycans on most CRC glycoproteins recognized by MGL	777:863	However, we failed to detect the presence of O-linked Tn and STn glycans on most CRC glycoproteins recognized by MGL.
32752259	1	28	theme	second-leading	159:172	arg1	cancer					139:144	Colorectal cancer	128:144	Colorectal cancer (CRC)	128:150	Colorectal cancer (CRC) is the second-leading cause of cancer death worldwide due in part to a high proportion of patients diagnosed at advanced stages of the disease.
32752259	1	28	theme	second-leading	159:172	arg1	cause					174:178	the second-leading cause	155:178	the second-leading cause of cancer death worldwide due in part to a high proportion of patients diagnosed at advanced stages of the disease	155:293	Colorectal cancer (CRC) is the second-leading cause of cancer death worldwide due in part to a high proportion of patients diagnosed at advanced stages of the disease.
32752259	0	29	theme	Major	23:27	arg1	Role					29:32	a Major Role	21:32	a Major Role	21:32	N-Glycoproteins Have a Major Role in MGL Binding to Colorectal Cancer Cell Lines: Associations with Overall Proteome Diversity.
32752259	1	30	theme	patients	242:249	arg1	proportion					228:237	a high proportion	221:237	a high proportion of patients diagnosed at advanced stages of the disease	221:293	Colorectal cancer (CRC) is the second-leading cause of cancer death worldwide due in part to a high proportion of patients diagnosed at advanced stages of the disease.
32752259	0	31	theme	Proteome	108:115	arg1	Diversity					117:125	Overall Proteome Diversity	100:125	Overall Proteome Diversity	100:125	N-Glycoproteins Have a Major Role in MGL Binding to Colorectal Cancer Cell Lines: Associations with Overall Proteome Diversity.
32752259	0	32	contain	Have	16:19	arg1	N-Glycoproteins					0:14	N-Glycoproteins	0:14	N-Glycoproteins	0:14	N-Glycoproteins Have a Major Role in MGL Binding to Colorectal Cancer Cell Lines: Associations with Overall Proteome Diversity.
32752259	0	32	contain	Have	16:19	arg2	Role					29:32	a Major Role	21:32	a Major Role	21:32	N-Glycoproteins Have a Major Role in MGL Binding to Colorectal Cancer Cell Lines: Associations with Overall Proteome Diversity.
32752259	3	33	theme	macrophage	503:512	arg1	MGL/CLEC10A/CD301					544:560	MGL/CLEC10A/CD301	544:560	MGL/CLEC10A/CD301	544:560	Cancer-associated aberrant glycosylation, especially the Tn and STn antigens, can be detected using the macrophage galactose-type C-type lectin (MGL/CLEC10A/CD301), which has been shown to be a promising tool for CRC prognosis.
32752259	3	33	theme	macrophage	503:512	arg1	tool					603:606	a promising tool	591:606	a promising tool for CRC prognosis	591:624	Cancer-associated aberrant glycosylation, especially the Tn and STn antigens, can be detected using the macrophage galactose-type C-type lectin (MGL/CLEC10A/CD301), which has been shown to be a promising tool for CRC prognosis.
32752259	3	33	theme	macrophage	503:512	arg1	lectin					536:541	the macrophage galactose-type C-type lectin	499:541	the macrophage galactose-type C-type lectin (MGL/CLEC10A/CD301)	499:561	Cancer-associated aberrant glycosylation, especially the Tn and STn antigens, can be detected using the macrophage galactose-type C-type lectin (MGL/CLEC10A/CD301), which has been shown to be a promising tool for CRC prognosis.
32752259	9	34	theme	differential	1370:1381	arg1	levels					1383:1388	differential levels	1370:1388	differential levels of enzymes involved in protein glycosylation	1370:1433	Finally, we highlighted both common and cell-specific processes associated with a high-MGL-binding phenotype, such as differential levels of enzymes involved in protein glycosylation, and a transcriptional factor (CDX-2) involved in their regulation.
32752259	2	35	theme	early	368:372	arg1	detection					374:382	early detection	368:382	early detection	368:382	For this reason, many efforts have been made towards new approaches for early detection and prognosis.
32752259	0	36	theme	Overall	100:106	arg1	Diversity					117:125	Overall Proteome Diversity	100:125	Overall Proteome Diversity	100:125	N-Glycoproteins Have a Major Role in MGL Binding to Colorectal Cancer Cell Lines: Associations with Overall Proteome Diversity.
32752259	3	37	theme	galactose-type	514:527	arg1	MGL/CLEC10A/CD301					544:560	MGL/CLEC10A/CD301	544:560	MGL/CLEC10A/CD301	544:560	Cancer-associated aberrant glycosylation, especially the Tn and STn antigens, can be detected using the macrophage galactose-type C-type lectin (MGL/CLEC10A/CD301), which has been shown to be a promising tool for CRC prognosis.
32752259	3	37	theme	galactose-type	514:527	arg1	tool					603:606	a promising tool	591:606	a promising tool for CRC prognosis	591:624	Cancer-associated aberrant glycosylation, especially the Tn and STn antigens, can be detected using the macrophage galactose-type C-type lectin (MGL/CLEC10A/CD301), which has been shown to be a promising tool for CRC prognosis.
32752259	3	37	theme	galactose-type	514:527	arg1	lectin					536:541	the macrophage galactose-type C-type lectin	499:541	the macrophage galactose-type C-type lectin (MGL/CLEC10A/CD301)	499:561	Cancer-associated aberrant glycosylation, especially the Tn and STn antigens, can be detected using the macrophage galactose-type C-type lectin (MGL/CLEC10A/CD301), which has been shown to be a promising tool for CRC prognosis.
32752259	5	38	theme	STn	809:811	arg1	glycans					813:819	STn glycans	809:819	STn glycans	809:819	However, we failed to detect the presence of O-linked Tn and STn glycans on most CRC glycoproteins recognized by MGL.
32752259	8	39	theme	cell	1240:1243	arg1	lines					1245:1249	CRC cell lines	1236:1249	CRC cell lines	1236:1249	Our results showed that N-glycans have a significant, previously underestimated, importance in MGL binding to CRC cell lines.
32752259	3	40	theme	Cancer-associated	399:415	arg1	Tn					456:457	Tn	456:457	Tn	456:457	Cancer-associated aberrant glycosylation, especially the Tn and STn antigens, can be detected using the macrophage galactose-type C-type lectin (MGL/CLEC10A/CD301), which has been shown to be a promising tool for CRC prognosis.
32752259	3	40	theme	Cancer-associated	399:415	arg1	antigens					467:474	STn antigens	463:474	STn antigens	463:474	Cancer-associated aberrant glycosylation, especially the Tn and STn antigens, can be detected using the macrophage galactose-type C-type lectin (MGL/CLEC10A/CD301), which has been shown to be a promising tool for CRC prognosis.
32752259	3	40	theme	Cancer-associated	399:415	arg1	glycosylation					426:438	Cancer-associated aberrant glycosylation	399:438	Cancer-associated aberrant glycosylation	399:438	Cancer-associated aberrant glycosylation, especially the Tn and STn antigens, can be detected using the macrophage galactose-type C-type lectin (MGL/CLEC10A/CD301), which has been shown to be a promising tool for CRC prognosis.
32752259	1	41	theme	cancer	183:188	arg1	worldwide					196:204	cancer death worldwide	183:204	cancer death worldwide due in part to a high proportion of patients diagnosed at advanced stages of the disease	183:293	Colorectal cancer (CRC) is the second-leading cause of cancer death worldwide due in part to a high proportion of patients diagnosed at advanced stages of the disease.
32752259	9	42	theme	cell-specific	1292:1304	arg1	levels					1383:1388	differential levels	1370:1388	differential levels of enzymes involved in protein glycosylation	1370:1433	Finally, we highlighted both common and cell-specific processes associated with a high-MGL-binding phenotype, such as differential levels of enzymes involved in protein glycosylation, and a transcriptional factor (CDX-2) involved in their regulation.
32752259	9	42	theme	cell-specific	1292:1304	arg1	factor					1458:1463	a transcriptional factor	1440:1463	a transcriptional factor (CDX-2) involved in their regulation	1440:1500	Finally, we highlighted both common and cell-specific processes associated with a high-MGL-binding phenotype, such as differential levels of enzymes involved in protein glycosylation, and a transcriptional factor (CDX-2) involved in their regulation.
32752259	9	42	theme	cell-specific	1292:1304	arg1	processes					1306:1314	both common and cell-specific processes	1276:1314	both common and cell-specific processes	1276:1314	Finally, we highlighted both common and cell-specific processes associated with a high-MGL-binding phenotype, such as differential levels of enzymes involved in protein glycosylation, and a transcriptional factor (CDX-2) involved in their regulation.
32752259	1	43	theme	advanced	264:271	arg1	stages					273:278	advanced stages	264:278	advanced stages of the disease	264:293	Colorectal cancer (CRC) is the second-leading cause of cancer death worldwide due in part to a high proportion of patients diagnosed at advanced stages of the disease.
32752259	1	44	theme	death	190:194	arg1	worldwide					196:204	cancer death worldwide	183:204	cancer death worldwide due in part to a high proportion of patients diagnosed at advanced stages of the disease	183:293	Colorectal cancer (CRC) is the second-leading cause of cancer death worldwide due in part to a high proportion of patients diagnosed at advanced stages of the disease.
32752259	8	45	from	importance	1207:1216	arg1	binding					1225:1231	MGL binding	1221:1231	MGL binding to CRC cell lines	1221:1249	Our results showed that N-glycans have a significant, previously underestimated, importance in MGL binding to CRC cell lines.
32752259	4	46	gly	glycoproteins	676:688	arg1	glycoproteins					676:688	the major MGL-binding glycoproteins	654:688	the major MGL-binding glycoproteins in two high-MGL-binding CRC cells lines, HCT116 and HT29	654:745	We had recently identified the major MGL-binding glycoproteins in two high-MGL-binding CRC cells lines, HCT116 and HT29.
32752259	5	47	from	presence	781:788	arg1	glycoproteins					833:845	most CRC glycoproteins	824:845	most CRC glycoproteins recognized by MGL	824:863	However, we failed to detect the presence of O-linked Tn and STn glycans on most CRC glycoproteins recognized by MGL.
32752259	5	48	theme	CRC	829:831	arg1	glycoproteins					833:845	most CRC glycoproteins	824:845	most CRC glycoproteins recognized by MGL	824:863	However, we failed to detect the presence of O-linked Tn and STn glycans on most CRC glycoproteins recognized by MGL.
32752259	0	49	theme	MGL	37:39	arg1	Binding					41:47	MGL Binding	37:47	MGL Binding to Colorectal Cancer Cell Lines	37:79	N-Glycoproteins Have a Major Role in MGL Binding to Colorectal Cancer Cell Lines: Associations with Overall Proteome Diversity.
32752259	9	50	theme	common	1281:1286	arg1	levels					1383:1388	differential levels	1370:1388	differential levels of enzymes involved in protein glycosylation	1370:1433	Finally, we highlighted both common and cell-specific processes associated with a high-MGL-binding phenotype, such as differential levels of enzymes involved in protein glycosylation, and a transcriptional factor (CDX-2) involved in their regulation.
32752259	9	50	theme	common	1281:1286	arg1	factor					1458:1463	a transcriptional factor	1440:1463	a transcriptional factor (CDX-2) involved in their regulation	1440:1500	Finally, we highlighted both common and cell-specific processes associated with a high-MGL-binding phenotype, such as differential levels of enzymes involved in protein glycosylation, and a transcriptional factor (CDX-2) involved in their regulation.
32752259	9	50	theme	common	1281:1286	arg1	processes					1306:1314	both common and cell-specific processes	1276:1314	both common and cell-specific processes	1276:1314	Finally, we highlighted both common and cell-specific processes associated with a high-MGL-binding phenotype, such as differential levels of enzymes involved in protein glycosylation, and a transcriptional factor (CDX-2) involved in their regulation.
32752259	1	51	from	part	213:216	arg1	due					206:208	due	206:208	due	206:208	Colorectal cancer (CRC) is the second-leading cause of cancer death worldwide due in part to a high proportion of patients diagnosed at advanced stages of the disease.
32752259	3	52	theme	CRC	612:614	arg1	prognosis					616:624	CRC prognosis	612:624	CRC prognosis	612:624	Cancer-associated aberrant glycosylation, especially the Tn and STn antigens, can be detected using the macrophage galactose-type C-type lectin (MGL/CLEC10A/CD301), which has been shown to be a promising tool for CRC prognosis.
32752259	9	53	theme	enzymes	1393:1399	arg1	CDX-2					1466:1470	CDX-2	1466:1470	CDX-2	1466:1470	Finally, we highlighted both common and cell-specific processes associated with a high-MGL-binding phenotype, such as differential levels of enzymes involved in protein glycosylation, and a transcriptional factor (CDX-2) involved in their regulation.
32752259	9	53	theme	enzymes	1393:1399	arg1	levels					1383:1388	differential levels	1370:1388	differential levels of enzymes involved in protein glycosylation	1370:1433	Finally, we highlighted both common and cell-specific processes associated with a high-MGL-binding phenotype, such as differential levels of enzymes involved in protein glycosylation, and a transcriptional factor (CDX-2) involved in their regulation.
32752259	9	53	theme	enzymes	1393:1399	arg1	factor					1458:1463	a transcriptional factor	1440:1463	a transcriptional factor (CDX-2) involved in their regulation	1440:1500	Finally, we highlighted both common and cell-specific processes associated with a high-MGL-binding phenotype, such as differential levels of enzymes involved in protein glycosylation, and a transcriptional factor (CDX-2) involved in their regulation.
32752259	4	54	from	glycoproteins	676:688	arg1	HT29					742:745	HT29	742:745	HT29	742:745	We had recently identified the major MGL-binding glycoproteins in two high-MGL-binding CRC cells lines, HCT116 and HT29.
32752259	4	54	from	glycoproteins	676:688	arg1	HCT116					731:736	HCT116	731:736	HCT116	731:736	We had recently identified the major MGL-binding glycoproteins in two high-MGL-binding CRC cells lines, HCT116 and HT29.
32752259	4	54	from	glycoproteins	676:688	arg1	lines					724:728	two high-MGL-binding CRC cells lines	693:728	two high-MGL-binding CRC cells lines	693:728	We had recently identified the major MGL-binding glycoproteins in two high-MGL-binding CRC cells lines, HCT116 and HT29.
32752259	6	55	link	O-linked	924:931	arg1	glycans					933:939	N-linked and O-linked glycans	911:939	N-linked and O-linked glycans carried by these proteins for the binding to MGL	911:988	We therefore investigated here the impact of N-linked and O-linked glycans carried by these proteins for the binding to MGL.
32752259	4	56	theme	MGL-binding	664:674	arg1	glycoproteins					676:688	the major MGL-binding glycoproteins	654:688	the major MGL-binding glycoproteins in two high-MGL-binding CRC cells lines, HCT116 and HT29	654:745	We had recently identified the major MGL-binding glycoproteins in two high-MGL-binding CRC cells lines, HCT116 and HT29.
32752259	4	57	theme	high-MGL-binding	697:712	arg1	HT29					742:745	HT29	742:745	HT29	742:745	We had recently identified the major MGL-binding glycoproteins in two high-MGL-binding CRC cells lines, HCT116 and HT29.
32752259	4	57	theme	high-MGL-binding	697:712	arg1	HCT116					731:736	HCT116	731:736	HCT116	731:736	We had recently identified the major MGL-binding glycoproteins in two high-MGL-binding CRC cells lines, HCT116 and HT29.
32752259	4	57	theme	high-MGL-binding	697:712	arg1	lines					724:728	two high-MGL-binding CRC cells lines	693:728	two high-MGL-binding CRC cells lines	693:728	We had recently identified the major MGL-binding glycoproteins in two high-MGL-binding CRC cells lines, HCT116 and HT29.
32752259	1	58	theme	worldwide	196:204	arg1	cancer					139:144	Colorectal cancer	128:144	Colorectal cancer (CRC)	128:150	Colorectal cancer (CRC) is the second-leading cause of cancer death worldwide due in part to a high proportion of patients diagnosed at advanced stages of the disease.
32752259	1	58	theme	worldwide	196:204	arg1	cause					174:178	the second-leading cause	155:178	the second-leading cause of cancer death worldwide due in part to a high proportion of patients diagnosed at advanced stages of the disease	155:293	Colorectal cancer (CRC) is the second-leading cause of cancer death worldwide due in part to a high proportion of patients diagnosed at advanced stages of the disease.
32752259	1	59	from	due	206:208	arg1	part					213:216	part	213:216	part	213:216	Colorectal cancer (CRC) is the second-leading cause of cancer death worldwide due in part to a high proportion of patients diagnosed at advanced stages of the disease.
32752259	5	60	attach	presence	781:788	arg1	glycoproteins					833:845	most CRC glycoproteins	824:845	most CRC glycoproteins recognized by MGL	824:863	However, we failed to detect the presence of O-linked Tn and STn glycans on most CRC glycoproteins recognized by MGL.
32752259	5	60	attach	presence	781:788	arg2	Tn					802:803	O-linked Tn	793:803	O-linked Tn	793:803	However, we failed to detect the presence of O-linked Tn and STn glycans on most CRC glycoproteins recognized by MGL.
32752259	5	60	attach	presence	781:788	arg2	glycans					813:819	STn glycans	809:819	STn glycans	809:819	However, we failed to detect the presence of O-linked Tn and STn glycans on most CRC glycoproteins recognized by MGL.
32752259	8	61	theme	CRC	1236:1238	arg1	lines					1245:1249	CRC cell lines	1236:1249	CRC cell lines	1236:1249	Our results showed that N-glycans have a significant, previously underestimated, importance in MGL binding to CRC cell lines.
32752259	4	62	theme	major	658:662	arg1	glycoproteins					676:688	the major MGL-binding glycoproteins	654:688	the major MGL-binding glycoproteins in two high-MGL-binding CRC cells lines, HCT116 and HT29	654:745	We had recently identified the major MGL-binding glycoproteins in two high-MGL-binding CRC cells lines, HCT116 and HT29.
32752259	1	63	theme	due	206:208	arg1	worldwide					196:204	cancer death worldwide	183:204	cancer death worldwide due in part to a high proportion of patients diagnosed at advanced stages of the disease	183:293	Colorectal cancer (CRC) is the second-leading cause of cancer death worldwide due in part to a high proportion of patients diagnosed at advanced stages of the disease.
32752259	0	64	theme	Colorectal	52:61	arg1	Lines					75:79	Colorectal Cancer Cell Lines	52:79	Colorectal Cancer Cell Lines	52:79	N-Glycoproteins Have a Major Role in MGL Binding to Colorectal Cancer Cell Lines: Associations with Overall Proteome Diversity.
32752259	2	65	theme	new	349:351	arg1	approaches					353:362	new approaches	349:362	new approaches for early detection and prognosis	349:396	For this reason, many efforts have been made towards new approaches for early detection and prognosis.
32752259	8	66	theme	underestimated	1191:1204	arg1	importance					1207:1216	a significant, previously underestimated, importance	1165:1216	importance	1207:1216	Our results showed that N-glycans have a significant, previously underestimated, importance in MGL binding to CRC cell lines.
32752259	1	67	theme	Colorectal	128:137	arg1	cancer					139:144	Colorectal cancer	128:144	Colorectal cancer (CRC)	128:150	Colorectal cancer (CRC) is the second-leading cause of cancer death worldwide due in part to a high proportion of patients diagnosed at advanced stages of the disease.
32752259	1	67	theme	Colorectal	128:137	arg1	cause					174:178	the second-leading cause	155:178	the second-leading cause of cancer death worldwide due in part to a high proportion of patients diagnosed at advanced stages of the disease	155:293	Colorectal cancer (CRC) is the second-leading cause of cancer death worldwide due in part to a high proportion of patients diagnosed at advanced stages of the disease.
32752259	1	67	theme	Colorectal	128:137	arg1	CRC					147:149	CRC	147:149	CRC	147:149	Colorectal cancer (CRC) is the second-leading cause of cancer death worldwide due in part to a high proportion of patients diagnosed at advanced stages of the disease.
32752259	6	68	theme	N-linked	911:918	arg1	glycans					933:939	N-linked and O-linked glycans	911:939	N-linked and O-linked glycans carried by these proteins for the binding to MGL	911:988	We therefore investigated here the impact of N-linked and O-linked glycans carried by these proteins for the binding to MGL.
32752259	1	69	theme	disease	287:293	arg1	stages					273:278	advanced stages	264:278	advanced stages of the disease	264:293	Colorectal cancer (CRC) is the second-leading cause of cancer death worldwide due in part to a high proportion of patients diagnosed at advanced stages of the disease.
32752259	2	70	theme	many	313:316	arg1	efforts					318:324	many efforts	313:324	many efforts	313:324	For this reason, many efforts have been made towards new approaches for early detection and prognosis.
32752259	6	71	theme	glycans	933:939	arg1	impact					901:906	the impact	897:906	the impact of N-linked and O-linked glycans carried by these proteins for the binding to MGL	897:988	We therefore investigated here the impact of N-linked and O-linked glycans carried by these proteins for the binding to MGL.
32726734	0	0	from	degradation	59:69	arg1	system					113:118	water-sediment system	98:118	water-sediment system of one eutrophic lake	98:140	Priming effect of autochthonous organic matter on enhanced degradation of 17α-ethynylestradiol in water-sediment system of one eutrophic lake.
32726734	6	1	theme	genes	1502:1506	arg1	upregulation					1467:1478	the clear upregulation	1457:1478	the clear upregulation of metabolic function genes of organic carbon and xenobiotics	1457:1540	In the meantime, the bacterial community composition was reshaped toward a more eutrophic state, leading to the clear upregulation of metabolic function genes of organic carbon and xenobiotics.
32726734	5	2	from	breakdown	989:997	arg1	MOM					1071:1073	MOM	1071:1073	MOM	1071:1073	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM remarkably augmented the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase, as well as the elevated production of proteins and polysaccharides in extracellular polymeric substances.
32726734	5	2	from	breakdown	989:997	arg1	COM					1063:1065	COM	1063:1065	COM	1063:1065	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM remarkably augmented the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase, as well as the elevated production of proteins and polysaccharides in extracellular polymeric substances.
32726734	4	3	theme	community	928:936	arg1	responses					894:902	responses	894:902	responses of indigenous bacterial community to OM amendment as a priming effect	894:972	The enhanced degradation was explained by responses of indigenous bacterial community to OM amendment as a priming effect.
32726734	8	4	from	management	1898:1907	arg1	lakes					1944:1948	freshwater lakes	1933:1948	freshwater lakes	1933:1948	These results suggest that cyanobacterial blooms-dominated zones are potential hotspot areas for steroid estrogen attenuation, a finding of significance for the control and management of complex pollution in freshwater lakes.
32726734	6	5	theme	bacterial	1370:1378	arg1	composition					1390:1400	the bacterial community composition	1366:1400	the bacterial community composition	1366:1400	In the meantime, the bacterial community composition was reshaped toward a more eutrophic state, leading to the clear upregulation of metabolic function genes of organic carbon and xenobiotics.
32726734	8	6	theme	potential	1794:1802	arg1	zones					1784:1788	cyanobacterial blooms-dominated zones	1752:1788	cyanobacterial blooms-dominated zones	1752:1788	These results suggest that cyanobacterial blooms-dominated zones are potential hotspot areas for steroid estrogen attenuation, a finding of significance for the control and management of complex pollution in freshwater lakes.
32726734	8	6	theme	potential	1794:1802	arg1	areas					1812:1816	potential hotspot areas	1794:1816	potential hotspot areas for steroid estrogen attenuation, a finding of significance	1794:1876	These results suggest that cyanobacterial blooms-dominated zones are potential hotspot areas for steroid estrogen attenuation, a finding of significance for the control and management of complex pollution in freshwater lakes.
32726734	8	7	from	control	1886:1892	arg1	lakes					1944:1948	freshwater lakes	1933:1948	freshwater lakes	1933:1948	These results suggest that cyanobacterial blooms-dominated zones are potential hotspot areas for steroid estrogen attenuation, a finding of significance for the control and management of complex pollution in freshwater lakes.
32726734	0	8	theme	autochthonous	18:30	arg1	matter					40:45	autochthonous organic matter	18:45	autochthonous organic matter	18:45	Priming effect of autochthonous organic matter on enhanced degradation of 17α-ethynylestradiol in water-sediment system of one eutrophic lake.
32726734	1	9	theme	autochthonous	228:240	arg1	OM					258:259	OM	258:259	OM	258:259	Climate change and increasing eutrophication are expected to increase the release of autochthonous organic matter (OM) to sediments, where most contaminants are transformed or mineralized in freshwater lakes.
32726734	1	9	theme	autochthonous	228:240	arg1	matter					250:255	autochthonous organic matter	228:255	autochthonous organic matter (OM)	228:260	Climate change and increasing eutrophication are expected to increase the release of autochthonous organic matter (OM) to sediments, where most contaminants are transformed or mineralized in freshwater lakes.
32726734	1	10	theme	most	282:285	arg1	contaminants					287:298	most contaminants	282:298	most contaminants	282:298	Climate change and increasing eutrophication are expected to increase the release of autochthonous organic matter (OM) to sediments, where most contaminants are transformed or mineralized in freshwater lakes.
32726734	5	11	from	production	1267:1276	arg1	terms					1134:1138	terms	1134:1138	terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase	1134:1240	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM remarkably augmented the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase, as well as the elevated production of proteins and polysaccharides in extracellular polymeric substances.
32726734	5	11	from	production	1267:1276	arg1	substances					1337:1346	extracellular polymeric substances	1313:1346	extracellular polymeric substances	1313:1346	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM remarkably augmented the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase, as well as the elevated production of proteins and polysaccharides in extracellular polymeric substances.
32726734	3	12	theme	water-sediment	575:588	arg1	experiments					600:610	water-sediment microcosm experiments	575:610	water-sediment microcosm experiments	575:610	In two months of water-sediment microcosm experiments, the input of COM and MOM both promoted EE2 degradation more strongly than humic acids, and the degradation efficiency was significantly and positively correlated with the cometabolism of increasing organic carbon in sediments (P < 0.001).
32726734	4	13	theme	indigenous	907:916	arg1	community					928:936	indigenous bacterial community	907:936	indigenous bacterial community	907:936	The enhanced degradation was explained by responses of indigenous bacterial community to OM amendment as a priming effect.
32726734	5	14	theme	extracellular	1313:1325	arg1	substances					1337:1346	extracellular polymeric substances	1313:1346	extracellular polymeric substances	1313:1346	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM remarkably augmented the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase, as well as the elevated production of proteins and polysaccharides in extracellular polymeric substances.
32726734	1	15	theme	Climate	143:149	arg1	change					151:156	Climate change	143:156	Climate change	143:156	Climate change and increasing eutrophication are expected to increase the release of autochthonous organic matter (OM) to sediments, where most contaminants are transformed or mineralized in freshwater lakes.
32726734	7	16	theme	compositional	1672:1684	arg1	variability					1686:1696	the compositional variability	1668:1696	the compositional variability of OM in the cometabolism	1668:1722	Correlation-based network analysis further determined the strong facilitative coordination between the community members and the compositional variability of OM in the cometabolism.
32726734	6	17	theme	eutrophic	1429:1437	arg1	state					1439:1443	a more eutrophic state	1422:1443	a more eutrophic state	1422:1443	In the meantime, the bacterial community composition was reshaped toward a more eutrophic state, leading to the clear upregulation of metabolic function genes of organic carbon and xenobiotics.
32726734	1	18	theme	freshwater	334:343	arg1	lakes					345:349	freshwater lakes	334:349	freshwater lakes	334:349	Climate change and increasing eutrophication are expected to increase the release of autochthonous organic matter (OM) to sediments, where most contaminants are transformed or mineralized in freshwater lakes.
32726734	8	19	from	lakes	1944:1948	arg1	control					1886:1892	control	1886:1892	control	1886:1892	These results suggest that cyanobacterial blooms-dominated zones are potential hotspot areas for steroid estrogen attenuation, a finding of significance for the control and management of complex pollution in freshwater lakes.
32726734	8	19	from	lakes	1944:1948	arg1	management					1898:1907	management	1898:1907	management	1898:1907	These results suggest that cyanobacterial blooms-dominated zones are potential hotspot areas for steroid estrogen attenuation, a finding of significance for the control and management of complex pollution in freshwater lakes.
32726734	5	20	theme	proteins	1281:1288	arg1	activity					1110:1117	the metabolic activity	1096:1117	the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase	1096:1240	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM remarkably augmented the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase, as well as the elevated production of proteins and polysaccharides in extracellular polymeric substances.
32726734	5	20	theme	proteins	1281:1288	arg1	production					1267:1276	the elevated production	1254:1276	the elevated production of proteins and polysaccharides in extracellular polymeric substances	1254:1346	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM remarkably augmented the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase, as well as the elevated production of proteins and polysaccharides in extracellular polymeric substances.
32726734	0	21	theme	17α-ethynylestradiol	74:93	arg1	degradation					59:69	enhanced degradation	50:69	enhanced degradation of 17α-ethynylestradiol in water-sediment system of one eutrophic lake	50:140	Priming effect of autochthonous organic matter on enhanced degradation of 17α-ethynylestradiol in water-sediment system of one eutrophic lake.
32726734	8	22	theme	complex	1912:1918	arg1	pollution					1920:1928	complex pollution	1912:1928	complex pollution in freshwater lakes	1912:1948	These results suggest that cyanobacterial blooms-dominated zones are potential hotspot areas for steroid estrogen attenuation, a finding of significance for the control and management of complex pollution in freshwater lakes.
32726734	7	23	theme	community	1646:1654	arg1	members					1656:1662	the community members	1642:1662	the community members	1642:1662	Correlation-based network analysis further determined the strong facilitative coordination between the community members and the compositional variability of OM in the cometabolism.
32726734	5	24	theme	polysaccharides	1294:1308	arg1	activity					1110:1117	the metabolic activity	1096:1117	the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase	1096:1240	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM remarkably augmented the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase, as well as the elevated production of proteins and polysaccharides in extracellular polymeric substances.
32726734	5	24	theme	polysaccharides	1294:1308	arg1	production					1267:1276	the elevated production	1254:1276	the elevated production of proteins and polysaccharides in extracellular polymeric substances	1254:1346	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM remarkably augmented the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase, as well as the elevated production of proteins and polysaccharides in extracellular polymeric substances.
32726734	0	25	theme	water-sediment	98:111	arg1	system					113:118	water-sediment system	98:118	water-sediment system of one eutrophic lake	98:140	Priming effect of autochthonous organic matter on enhanced degradation of 17α-ethynylestradiol in water-sediment system of one eutrophic lake.
32726734	4	26	theme	priming	959:965	arg1	effect					967:972	a priming effect	957:972	a priming effect	957:972	The enhanced degradation was explained by responses of indigenous bacterial community to OM amendment as a priming effect.
32726734	2	27	theme	macrophyte-derived	405:422	arg1	OM					424:425	macrophyte-derived OM	405:425	macrophyte-derived OM (COM and MOM)	405:439	This study sought to evaluate how cyanobacteria- and macrophyte-derived OM (COM and MOM) affected the microbial attenuation of 17α-ethinylestradiol (EE2) in the sediment from eutrophic Lake Taihu in China.
32726734	5	28	theme	bacteria	1122:1129	arg1	activity					1110:1117	the metabolic activity	1096:1117	the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase	1096:1240	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM remarkably augmented the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase, as well as the elevated production of proteins and polysaccharides in extracellular polymeric substances.
32726734	5	28	theme	bacteria	1122:1129	arg1	production					1267:1276	the elevated production	1254:1276	the elevated production of proteins and polysaccharides in extracellular polymeric substances	1254:1346	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM remarkably augmented the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase, as well as the elevated production of proteins and polysaccharides in extracellular polymeric substances.
32726734	5	29	theme	immediate	979:987	arg1	breakdown					989:997	The immediate breakdown	975:997	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM	975:1073	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM remarkably augmented the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase, as well as the elevated production of proteins and polysaccharides in extracellular polymeric substances.
32726734	0	30	theme	Priming	0:6	arg1	effect					8:13	Priming effect	0:13	Priming effect of autochthonous organic matter on enhanced degradation of 17α-ethynylestradiol in water-sediment system of one eutrophic lake.	0:141	Priming effect of autochthonous organic matter on enhanced degradation of 17α-ethynylestradiol in water-sediment system of one eutrophic lake.
32726734	6	31	theme	clear	1461:1465	arg1	upregulation					1467:1478	the clear upregulation	1457:1478	the clear upregulation of metabolic function genes of organic carbon and xenobiotics	1457:1540	In the meantime, the bacterial community composition was reshaped toward a more eutrophic state, leading to the clear upregulation of metabolic function genes of organic carbon and xenobiotics.
32726734	7	32	theme	strong	1601:1606	arg1	coordination					1621:1632	the strong facilitative coordination	1597:1632	the strong facilitative coordination between the community members	1597:1662	Correlation-based network analysis further determined the strong facilitative coordination between the community members and the compositional variability of OM in the cometabolism.
32726734	2	33	theme	17α-ethinylestradiol	479:498	arg1	attenuation					464:474	the microbial attenuation	450:474	the microbial attenuation of 17α-ethinylestradiol (EE2) in the sediment from eutrophic Lake Taihu in China	450:555	This study sought to evaluate how cyanobacteria- and macrophyte-derived OM (COM and MOM) affected the microbial attenuation of 17α-ethinylestradiol (EE2) in the sediment from eutrophic Lake Taihu in China.
32726734	0	34	theme	organic	32:38	arg1	matter					40:45	autochthonous organic matter	18:45	autochthonous organic matter	18:45	Priming effect of autochthonous organic matter on enhanced degradation of 17α-ethynylestradiol in water-sediment system of one eutrophic lake.
32726734	5	35	theme	activity	1158:1165	arg1	terms					1134:1138	terms	1134:1138	terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase	1134:1240	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM remarkably augmented the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase, as well as the elevated production of proteins and polysaccharides in extracellular polymeric substances.
32726734	1	36	from	lakes	345:349	arg1	transformed					304:314	transformed	304:314	transformed	304:314	Climate change and increasing eutrophication are expected to increase the release of autochthonous organic matter (OM) to sediments, where most contaminants are transformed or mineralized in freshwater lakes.
32726734	5	37	theme	extracellular	1170:1182	arg1	dehydrogenase					1228:1240	dehydrogenase	1228:1240	dehydrogenase	1228:1240	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM remarkably augmented the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase, as well as the elevated production of proteins and polysaccharides in extracellular polymeric substances.
32726734	5	37	theme	extracellular	1170:1182	arg1	enzymes					1184:1190	extracellular enzymes	1170:1190	extracellular enzymes including fluorescein diacetate and dehydrogenase	1170:1240	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM remarkably augmented the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase, as well as the elevated production of proteins and polysaccharides in extracellular polymeric substances.
32726734	5	37	theme	extracellular	1170:1182	arg1	diacetate					1214:1222	fluorescein diacetate	1202:1222	fluorescein diacetate	1202:1222	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM remarkably augmented the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase, as well as the elevated production of proteins and polysaccharides in extracellular polymeric substances.
32726734	3	38	theme	COM	626:628	arg1	input					617:621	the input	613:621	the input of COM and MOM	613:636	In two months of water-sediment microcosm experiments, the input of COM and MOM both promoted EE2 degradation more strongly than humic acids, and the degradation efficiency was significantly and positively correlated with the cometabolism of increasing organic carbon in sediments (P < 0.001).
32726734	6	39	theme	xenobiotics	1530:1540	arg1	genes					1502:1506	metabolic function genes	1483:1506	metabolic function genes of organic carbon and xenobiotics	1483:1540	In the meantime, the bacterial community composition was reshaped toward a more eutrophic state, leading to the clear upregulation of metabolic function genes of organic carbon and xenobiotics.
32726734	1	40	theme	organic	242:248	arg1	OM					258:259	OM	258:259	OM	258:259	Climate change and increasing eutrophication are expected to increase the release of autochthonous organic matter (OM) to sediments, where most contaminants are transformed or mineralized in freshwater lakes.
32726734	1	40	theme	organic	242:248	arg1	matter					250:255	autochthonous organic matter	228:255	autochthonous organic matter (OM)	228:260	Climate change and increasing eutrophication are expected to increase the release of autochthonous organic matter (OM) to sediments, where most contaminants are transformed or mineralized in freshwater lakes.
32726734	7	41	theme	Correlation-based	1543:1559	arg1	analysis					1569:1576	Correlation-based network analysis	1543:1576	Correlation-based network analysis	1543:1576	Correlation-based network analysis further determined the strong facilitative coordination between the community members and the compositional variability of OM in the cometabolism.
32726734	8	42	dep	control	1886:1892	arg1	the					1882:1884	the	1882:1884	the	1882:1884	These results suggest that cyanobacterial blooms-dominated zones are potential hotspot areas for steroid estrogen attenuation, a finding of significance for the control and management of complex pollution in freshwater lakes.
32726734	8	43	theme	steroid	1822:1828	arg1	attenuation					1839:1849	steroid estrogen attenuation	1822:1849	steroid estrogen attenuation	1822:1849	These results suggest that cyanobacterial blooms-dominated zones are potential hotspot areas for steroid estrogen attenuation, a finding of significance for the control and management of complex pollution in freshwater lakes.
32726734	8	43	theme	steroid	1822:1828	arg1	finding					1854:1860	a finding	1852:1860	a finding of significance	1852:1876	These results suggest that cyanobacterial blooms-dominated zones are potential hotspot areas for steroid estrogen attenuation, a finding of significance for the control and management of complex pollution in freshwater lakes.
32726734	3	44	theme	EE2	652:654	arg1	degradation					656:666	EE2 degradation	652:666	EE2 degradation	652:666	In two months of water-sediment microcosm experiments, the input of COM and MOM both promoted EE2 degradation more strongly than humic acids, and the degradation efficiency was significantly and positively correlated with the cometabolism of increasing organic carbon in sediments (P < 0.001).
32726734	6	45	theme	carbon	1519:1524	arg1	genes					1502:1506	metabolic function genes	1483:1506	metabolic function genes of organic carbon and xenobiotics	1483:1540	In the meantime, the bacterial community composition was reshaped toward a more eutrophic state, leading to the clear upregulation of metabolic function genes of organic carbon and xenobiotics.
32726734	5	46	theme	elevated	1258:1265	arg1	production					1267:1276	the elevated production	1254:1276	the elevated production of proteins and polysaccharides in extracellular polymeric substances	1254:1346	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM remarkably augmented the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase, as well as the elevated production of proteins and polysaccharides in extracellular polymeric substances.
32726734	5	47	theme	components	1016:1025	arg1	breakdown					989:997	The immediate breakdown	975:997	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM	975:1073	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM remarkably augmented the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase, as well as the elevated production of proteins and polysaccharides in extracellular polymeric substances.
32726734	8	48	theme	blooms-dominated	1767:1782	arg1	zones					1784:1788	cyanobacterial blooms-dominated zones	1752:1788	cyanobacterial blooms-dominated zones	1752:1788	These results suggest that cyanobacterial blooms-dominated zones are potential hotspot areas for steroid estrogen attenuation, a finding of significance for the control and management of complex pollution in freshwater lakes.
32726734	8	48	theme	blooms-dominated	1767:1782	arg1	areas					1812:1816	potential hotspot areas	1794:1816	potential hotspot areas for steroid estrogen attenuation, a finding of significance	1794:1876	These results suggest that cyanobacterial blooms-dominated zones are potential hotspot areas for steroid estrogen attenuation, a finding of significance for the control and management of complex pollution in freshwater lakes.
32726734	3	49	dep	promoted	643:650	arg1	both					638:641	both	638:641	both	638:641	In two months of water-sediment microcosm experiments, the input of COM and MOM both promoted EE2 degradation more strongly than humic acids, and the degradation efficiency was significantly and positively correlated with the cometabolism of increasing organic carbon in sediments (P < 0.001).
32726734	4	50	theme	enhanced	856:863	arg1	degradation					865:875	The enhanced degradation	852:875	The enhanced degradation	852:875	The enhanced degradation was explained by responses of indigenous bacterial community to OM amendment as a priming effect.
32726734	1	51	from	transformed	304:314	arg1	lakes					345:349	freshwater lakes	334:349	freshwater lakes	334:349	Climate change and increasing eutrophication are expected to increase the release of autochthonous organic matter (OM) to sediments, where most contaminants are transformed or mineralized in freshwater lakes.
32726734	6	52	theme	function	1493:1500	arg1	genes					1502:1506	metabolic function genes	1483:1506	metabolic function genes of organic carbon and xenobiotics	1483:1540	In the meantime, the bacterial community composition was reshaped toward a more eutrophic state, leading to the clear upregulation of metabolic function genes of organic carbon and xenobiotics.
32726734	3	53	theme	degradation	708:718	arg1	efficiency					720:729	the degradation efficiency	704:729	the degradation efficiency	704:729	In two months of water-sediment microcosm experiments, the input of COM and MOM both promoted EE2 degradation more strongly than humic acids, and the degradation efficiency was significantly and positively correlated with the cometabolism of increasing organic carbon in sediments (P < 0.001).
32726734	8	54	theme	hotspot	1804:1810	arg1	zones					1784:1788	cyanobacterial blooms-dominated zones	1752:1788	cyanobacterial blooms-dominated zones	1752:1788	These results suggest that cyanobacterial blooms-dominated zones are potential hotspot areas for steroid estrogen attenuation, a finding of significance for the control and management of complex pollution in freshwater lakes.
32726734	8	54	theme	hotspot	1804:1810	arg1	areas					1812:1816	potential hotspot areas	1794:1816	potential hotspot areas for steroid estrogen attenuation, a finding of significance	1794:1876	These results suggest that cyanobacterial blooms-dominated zones are potential hotspot areas for steroid estrogen attenuation, a finding of significance for the control and management of complex pollution in freshwater lakes.
32726734	6	55	theme	community	1380:1388	arg1	composition					1390:1400	the bacterial community composition	1366:1400	the bacterial community composition	1366:1400	In the meantime, the bacterial community composition was reshaped toward a more eutrophic state, leading to the clear upregulation of metabolic function genes of organic carbon and xenobiotics.
32726734	8	56	theme	estrogen	1830:1837	arg1	attenuation					1839:1849	steroid estrogen attenuation	1822:1849	steroid estrogen attenuation	1822:1849	These results suggest that cyanobacterial blooms-dominated zones are potential hotspot areas for steroid estrogen attenuation, a finding of significance for the control and management of complex pollution in freshwater lakes.
32726734	8	56	theme	estrogen	1830:1837	arg1	finding					1854:1860	a finding	1852:1860	a finding of significance	1852:1876	These results suggest that cyanobacterial blooms-dominated zones are potential hotspot areas for steroid estrogen attenuation, a finding of significance for the control and management of complex pollution in freshwater lakes.
32726734	4	57	theme	bacterial	918:926	arg1	community					928:936	indigenous bacterial community	907:936	indigenous bacterial community	907:936	The enhanced degradation was explained by responses of indigenous bacterial community to OM amendment as a priming effect.
32726734	3	58	theme	microcosm	590:598	arg1	experiments					600:610	water-sediment microcosm experiments	575:610	water-sediment microcosm experiments	575:610	In two months of water-sediment microcosm experiments, the input of COM and MOM both promoted EE2 degradation more strongly than humic acids, and the degradation efficiency was significantly and positively correlated with the cometabolism of increasing organic carbon in sediments (P < 0.001).
32726734	7	59	theme	OM	1701:1702	arg1	coordination					1621:1632	the strong facilitative coordination	1597:1632	the strong facilitative coordination between the community members	1597:1662	Correlation-based network analysis further determined the strong facilitative coordination between the community members and the compositional variability of OM in the cometabolism.
32726734	7	59	theme	OM	1701:1702	arg1	variability					1686:1696	the compositional variability	1668:1696	the compositional variability of OM in the cometabolism	1668:1722	Correlation-based network analysis further determined the strong facilitative coordination between the community members and the compositional variability of OM in the cometabolism.
32726734	5	60	theme	metabolic	1100:1108	arg1	activity					1110:1117	the metabolic activity	1096:1117	the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase	1096:1240	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM remarkably augmented the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase, as well as the elevated production of proteins and polysaccharides in extracellular polymeric substances.
32726734	0	61	theme	lake	137:140	arg1	system					113:118	water-sediment system	98:118	water-sediment system of one eutrophic lake	98:140	Priming effect of autochthonous organic matter on enhanced degradation of 17α-ethynylestradiol in water-sediment system of one eutrophic lake.
32726734	2	62	from	sediment	513:520	arg1	attenuation					464:474	the microbial attenuation	450:474	the microbial attenuation of 17α-ethinylestradiol (EE2) in the sediment from eutrophic Lake Taihu in China	450:555	This study sought to evaluate how cyanobacteria- and macrophyte-derived OM (COM and MOM) affected the microbial attenuation of 17α-ethinylestradiol (EE2) in the sediment from eutrophic Lake Taihu in China.
32726734	8	63	from	pollution	1920:1928	arg1	lakes					1944:1948	freshwater lakes	1933:1948	freshwater lakes	1933:1948	These results suggest that cyanobacterial blooms-dominated zones are potential hotspot areas for steroid estrogen attenuation, a finding of significance for the control and management of complex pollution in freshwater lakes.
32726734	8	64	theme	significance	1865:1876	arg1	attenuation					1839:1849	steroid estrogen attenuation	1822:1849	steroid estrogen attenuation	1822:1849	These results suggest that cyanobacterial blooms-dominated zones are potential hotspot areas for steroid estrogen attenuation, a finding of significance for the control and management of complex pollution in freshwater lakes.
32726734	8	64	theme	significance	1865:1876	arg1	finding					1854:1860	a finding	1852:1860	a finding of significance	1852:1876	These results suggest that cyanobacterial blooms-dominated zones are potential hotspot areas for steroid estrogen attenuation, a finding of significance for the control and management of complex pollution in freshwater lakes.
32726734	5	65	theme	polymeric	1327:1335	arg1	substances					1337:1346	extracellular polymeric substances	1313:1346	extracellular polymeric substances	1313:1346	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM remarkably augmented the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase, as well as the elevated production of proteins and polysaccharides in extracellular polymeric substances.
32726734	3	66	theme	organic	811:817	arg1	carbon					819:824	organic carbon	811:824	organic carbon	811:824	In two months of water-sediment microcosm experiments, the input of COM and MOM both promoted EE2 degradation more strongly than humic acids, and the degradation efficiency was significantly and positively correlated with the cometabolism of increasing organic carbon in sediments (P < 0.001).
32726734	7	67	from	variability	1686:1696	arg1	cometabolism					1711:1722	the cometabolism	1707:1722	the cometabolism	1707:1722	Correlation-based network analysis further determined the strong facilitative coordination between the community members and the compositional variability of OM in the cometabolism.
32726734	1	68	theme	increasing	162:171	arg1	eutrophication					173:186	increasing eutrophication	162:186	increasing eutrophication	162:186	Climate change and increasing eutrophication are expected to increase the release of autochthonous organic matter (OM) to sediments, where most contaminants are transformed or mineralized in freshwater lakes.
32726734	0	69	theme	eutrophic	127:135	arg1	lake					137:140	one eutrophic lake	123:140	one eutrophic lake	123:140	Priming effect of autochthonous organic matter on enhanced degradation of 17α-ethynylestradiol in water-sediment system of one eutrophic lake.
32726734	0	70	from	effect	8:13	arg1	degradation					59:69	enhanced degradation	50:69	enhanced degradation of 17α-ethynylestradiol in water-sediment system of one eutrophic lake	50:140	Priming effect of autochthonous organic matter on enhanced degradation of 17α-ethynylestradiol in water-sediment system of one eutrophic lake.
32726734	5	71	theme	stimulated	1147:1156	arg1	activity					1158:1165	the stimulated activity	1143:1165	the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase	1143:1240	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM remarkably augmented the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase, as well as the elevated production of proteins and polysaccharides in extracellular polymeric substances.
32726734	2	72	from	attenuation	464:474	arg1	sediment					513:520	the sediment	509:520	the sediment from eutrophic Lake Taihu in China	509:555	This study sought to evaluate how cyanobacteria- and macrophyte-derived OM (COM and MOM) affected the microbial attenuation of 17α-ethinylestradiol (EE2) in the sediment from eutrophic Lake Taihu in China.
32726734	8	73	theme	pollution	1920:1928	arg1	control					1886:1892	control	1886:1892	control	1886:1892	These results suggest that cyanobacterial blooms-dominated zones are potential hotspot areas for steroid estrogen attenuation, a finding of significance for the control and management of complex pollution in freshwater lakes.
32726734	8	73	theme	pollution	1920:1928	arg1	management					1898:1907	management	1898:1907	management	1898:1907	These results suggest that cyanobacterial blooms-dominated zones are potential hotspot areas for steroid estrogen attenuation, a finding of significance for the control and management of complex pollution in freshwater lakes.
32726734	2	74	from	17α-ethinylestradiol	479:498	arg1	sediment					513:520	the sediment	509:520	the sediment from eutrophic Lake Taihu in China	509:555	This study sought to evaluate how cyanobacteria- and macrophyte-derived OM (COM and MOM) affected the microbial attenuation of 17α-ethinylestradiol (EE2) in the sediment from eutrophic Lake Taihu in China.
32726734	2	74	from	17α-ethinylestradiol	479:498	arg1	Taihu					542:546	Taihu	542:546	Taihu	542:546	This study sought to evaluate how cyanobacteria- and macrophyte-derived OM (COM and MOM) affected the microbial attenuation of 17α-ethinylestradiol (EE2) in the sediment from eutrophic Lake Taihu in China.
32726734	6	75	theme	metabolic	1483:1491	arg1	genes					1502:1506	metabolic function genes	1483:1506	metabolic function genes of organic carbon and xenobiotics	1483:1540	In the meantime, the bacterial community composition was reshaped toward a more eutrophic state, leading to the clear upregulation of metabolic function genes of organic carbon and xenobiotics.
32726734	8	76	theme	freshwater	1933:1942	arg1	lakes					1944:1948	freshwater lakes	1933:1948	freshwater lakes	1933:1948	These results suggest that cyanobacterial blooms-dominated zones are potential hotspot areas for steroid estrogen attenuation, a finding of significance for the control and management of complex pollution in freshwater lakes.
32726734	5	77	from	activity	1110:1117	arg1	terms					1134:1138	terms	1134:1138	terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase	1134:1240	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM remarkably augmented the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase, as well as the elevated production of proteins and polysaccharides in extracellular polymeric substances.
32726734	5	77	from	activity	1110:1117	arg1	substances					1337:1346	extracellular polymeric substances	1313:1346	extracellular polymeric substances	1313:1346	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM remarkably augmented the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase, as well as the elevated production of proteins and polysaccharides in extracellular polymeric substances.
32726734	5	78	theme	fluorescein	1202:1212	arg1	diacetate					1214:1222	fluorescein diacetate	1202:1222	fluorescein diacetate	1202:1222	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM remarkably augmented the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase, as well as the elevated production of proteins and polysaccharides in extracellular polymeric substances.
32726734	7	79	from	coordination	1621:1632	arg1	cometabolism					1711:1722	the cometabolism	1707:1722	the cometabolism	1707:1722	Correlation-based network analysis further determined the strong facilitative coordination between the community members and the compositional variability of OM in the cometabolism.
32726734	3	80	theme	MOM	634:636	arg1	input					617:621	the input	613:621	the input of COM and MOM	613:636	In two months of water-sediment microcosm experiments, the input of COM and MOM both promoted EE2 degradation more strongly than humic acids, and the degradation efficiency was significantly and positively correlated with the cometabolism of increasing organic carbon in sediments (P < 0.001).
32726734	0	81	theme	matter	40:45	arg1	effect					8:13	Priming effect	0:13	Priming effect of autochthonous organic matter on enhanced degradation of 17α-ethynylestradiol in water-sediment system of one eutrophic lake.	0:141	Priming effect of autochthonous organic matter on enhanced degradation of 17α-ethynylestradiol in water-sediment system of one eutrophic lake.
32726734	7	82	theme	facilitative	1608:1619	arg1	coordination					1621:1632	the strong facilitative coordination	1597:1632	the strong facilitative coordination between the community members	1597:1662	Correlation-based network analysis further determined the strong facilitative coordination between the community members and the compositional variability of OM in the cometabolism.
32726734	2	83	link	macrophyte-derived	405:422	arg1	OM					424:425	macrophyte-derived OM	405:425	macrophyte-derived OM (COM and MOM)	405:439	This study sought to evaluate how cyanobacteria- and macrophyte-derived OM (COM and MOM) affected the microbial attenuation of 17α-ethinylestradiol (EE2) in the sediment from eutrophic Lake Taihu in China.
32726734	2	84	dep	OM	424:425	arg1	MOM					436:438	MOM	436:438	MOM	436:438	This study sought to evaluate how cyanobacteria- and macrophyte-derived OM (COM and MOM) affected the microbial attenuation of 17α-ethinylestradiol (EE2) in the sediment from eutrophic Lake Taihu in China.
32726734	2	84	dep	OM	424:425	arg1	COM					428:430	COM	428:430	COM	428:430	This study sought to evaluate how cyanobacteria- and macrophyte-derived OM (COM and MOM) affected the microbial attenuation of 17α-ethinylestradiol (EE2) in the sediment from eutrophic Lake Taihu in China.
32726734	0	85	theme	enhanced	50:57	arg1	degradation					59:69	enhanced degradation	50:69	enhanced degradation of 17α-ethynylestradiol in water-sediment system of one eutrophic lake	50:140	Priming effect of autochthonous organic matter on enhanced degradation of 17α-ethynylestradiol in water-sediment system of one eutrophic lake.
32726734	5	86	theme	proteinaceous	1035:1047	arg1	substances					1049:1058	proteinaceous substances	1035:1058	proteinaceous substances	1035:1058	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM remarkably augmented the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase, as well as the elevated production of proteins and polysaccharides in extracellular polymeric substances.
32726734	2	87	theme	microbial	454:462	arg1	attenuation					464:474	the microbial attenuation	450:474	the microbial attenuation of 17α-ethinylestradiol (EE2) in the sediment from eutrophic Lake Taihu in China	450:555	This study sought to evaluate how cyanobacteria- and macrophyte-derived OM (COM and MOM) affected the microbial attenuation of 17α-ethinylestradiol (EE2) in the sediment from eutrophic Lake Taihu in China.
32726734	5	88	theme	enzymes	1184:1190	arg1	activity					1158:1165	the stimulated activity	1143:1165	the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase	1143:1240	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM remarkably augmented the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase, as well as the elevated production of proteins and polysaccharides in extracellular polymeric substances.
32726734	3	89	theme	experiments	600:610	arg1	months					565:570	two months	561:570	two months of water-sediment microcosm experiments	561:610	In two months of water-sediment microcosm experiments, the input of COM and MOM both promoted EE2 degradation more strongly than humic acids, and the degradation efficiency was significantly and positively correlated with the cometabolism of increasing organic carbon in sediments (P < 0.001).
32726734	2	90	from	Taihu	542:546	arg1	sediment					513:520	the sediment	509:520	the sediment from eutrophic Lake Taihu in China	509:555	This study sought to evaluate how cyanobacteria- and macrophyte-derived OM (COM and MOM) affected the microbial attenuation of 17α-ethinylestradiol (EE2) in the sediment from eutrophic Lake Taihu in China.
32726734	2	90	from	Taihu	542:546	arg1	EE2					501:503	EE2	501:503	EE2	501:503	This study sought to evaluate how cyanobacteria- and macrophyte-derived OM (COM and MOM) affected the microbial attenuation of 17α-ethinylestradiol (EE2) in the sediment from eutrophic Lake Taihu in China.
32726734	2	90	from	Taihu	542:546	arg1	17α-ethinylestradiol					479:498	17α-ethinylestradiol	479:498	17α-ethinylestradiol (EE2) in the sediment from eutrophic Lake Taihu in China	479:555	This study sought to evaluate how cyanobacteria- and macrophyte-derived OM (COM and MOM) affected the microbial attenuation of 17α-ethinylestradiol (EE2) in the sediment from eutrophic Lake Taihu in China.
32726734	8	91	theme	cyanobacterial	1752:1765	arg1	zones					1784:1788	cyanobacterial blooms-dominated zones	1752:1788	cyanobacterial blooms-dominated zones	1752:1788	These results suggest that cyanobacterial blooms-dominated zones are potential hotspot areas for steroid estrogen attenuation, a finding of significance for the control and management of complex pollution in freshwater lakes.
32726734	8	91	theme	cyanobacterial	1752:1765	arg1	areas					1812:1816	potential hotspot areas	1794:1816	potential hotspot areas for steroid estrogen attenuation, a finding of significance	1794:1876	These results suggest that cyanobacterial blooms-dominated zones are potential hotspot areas for steroid estrogen attenuation, a finding of significance for the control and management of complex pollution in freshwater lakes.
32726734	1	92	theme	matter	250:255	arg1	release					217:223	the release	213:223	the release of autochthonous organic matter (OM) to sediments, where most contaminants are transformed or mineralized in freshwater lakes	213:349	Climate change and increasing eutrophication are expected to increase the release of autochthonous organic matter (OM) to sediments, where most contaminants are transformed or mineralized in freshwater lakes.
32726734	7	93	theme	network	1561:1567	arg1	analysis					1569:1576	Correlation-based network analysis	1543:1576	Correlation-based network analysis	1543:1576	Correlation-based network analysis further determined the strong facilitative coordination between the community members and the compositional variability of OM in the cometabolism.
32726734	5	94	theme	biodegradable	1002:1014	arg1	components					1016:1025	biodegradable components	1002:1025	biodegradable components such as proteinaceous substances	1002:1058	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM remarkably augmented the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase, as well as the elevated production of proteins and polysaccharides in extracellular polymeric substances.
32726734	5	94	theme	biodegradable	1002:1014	arg1	substances					1049:1058	proteinaceous substances	1035:1058	proteinaceous substances	1035:1058	The immediate breakdown of biodegradable components such as proteinaceous substances in COM and MOM remarkably augmented the metabolic activity of bacteria in terms of the stimulated activity of extracellular enzymes including fluorescein diacetate and dehydrogenase, as well as the elevated production of proteins and polysaccharides in extracellular polymeric substances.
32726734	3	95	theme	humic	687:691	arg1	acids					693:697	humic acids	687:697	humic acids	687:697	In two months of water-sediment microcosm experiments, the input of COM and MOM both promoted EE2 degradation more strongly than humic acids, and the degradation efficiency was significantly and positively correlated with the cometabolism of increasing organic carbon in sediments (P < 0.001).
32726734	6	96	theme	organic	1511:1517	arg1	carbon					1519:1524	organic carbon	1511:1524	organic carbon	1511:1524	In the meantime, the bacterial community composition was reshaped toward a more eutrophic state, leading to the clear upregulation of metabolic function genes of organic carbon and xenobiotics.
34904944	12	0	theme	polyamine	1335:1343	arg1	pattern					1345:1351	The polyamine pattern	1331:1351	The polyamine pattern	1331:1351	The polyamine pattern contained major amounts of spermidine and spermine.
34904944	11	1	theme	phospholipids	1248:1260	arg1	amounts					1220:1226	minor amounts	1214:1226	minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid	1214:1328	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid.
34904944	11	1	theme	phospholipids	1248:1260	arg1	aminophospholipid					1312:1328	aminophospholipid	1312:1328	aminophospholipid	1312:1328	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid.
34904944	11	1	theme	phospholipids	1248:1260	arg1	phospholipids					1248:1260	two unidentified phospholipids	1231:1260	two unidentified phospholipids	1231:1260	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid.
34904944	11	1	theme	phospholipids	1248:1260	arg1	glycolipids					1280:1290	two unidentified glycolipids	1263:1290	two unidentified glycolipids	1263:1290	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid.
34904944	11	2	theme	major	1140:1144	arg1	lipids					1146:1151	the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid	1136:1328	the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid	1136:1328	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid.
34904944	11	2	theme	major	1140:1144	arg1	phosphatidylglycerol					1177:1196	phosphatidylglycerol	1177:1196	phosphatidylglycerol	1177:1196	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid.
34904944	11	2	theme	major	1140:1144	arg1	diphosphatidylglycerol					1153:1174	diphosphatidylglycerol	1153:1174	diphosphatidylglycerol	1153:1174	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid.
34904944	14	3	theme	analyses	1576:1583	arg1	results					1552:1558	The results	1548:1558	The results of ANI and dDDH analyses and physiological and biochemical tests	1548:1623	The results of ANI and dDDH analyses and physiological and biochemical tests allowed a genotypic and phenotypic differentiation of strain IMT-300T from the most closely related Leucobacter species.
34904944	4	4	theme	similarity	317:326	arg1	studies					328:334	16S rRNA gene sequence similarity studies	294:334	16S rRNA gene sequence similarity studies	294:334	Based on 16S rRNA gene sequence similarity studies, strain IMT-300T was shown to belong to the genus Leucobacter and was closely related to the type strain of 'Leucobacter margaritiformis' L1T (97.8%).
34904944	6	5	theme	genome	677:682	arg1	assembly					684:691	the IMT-300T genome assembly	664:691	the IMT-300T genome assembly	664:691	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the IMT-300T genome assembly and those of the closest relative Leucobacter type strain were 81.4 and 23.3 % (Leucobacter chironomi), respectively.
34904944	6	6	theme	average	574:580	arg1	ANI					603:605	ANI	603:605	ANI	603:605	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the IMT-300T genome assembly and those of the closest relative Leucobacter type strain were 81.4 and 23.3 % (Leucobacter chironomi), respectively.
34904944	6	6	theme	average	574:580	arg1	identity					593:600	The average nucleotide identity	570:600	The average nucleotide identity (ANI)	570:606	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the IMT-300T genome assembly and those of the closest relative Leucobacter type strain were 81.4 and 23.3 % (Leucobacter chironomi), respectively.
34904944	11	7	theme	glycolipids	1280:1290	arg1	amounts					1220:1226	minor amounts	1214:1226	minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid	1214:1328	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid.
34904944	11	7	theme	glycolipids	1280:1290	arg1	aminophospholipid					1312:1328	aminophospholipid	1312:1328	aminophospholipid	1312:1328	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid.
34904944	11	7	theme	glycolipids	1280:1290	arg1	phospholipids					1248:1260	two unidentified phospholipids	1231:1260	two unidentified phospholipids	1231:1260	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid.
34904944	11	7	theme	glycolipids	1280:1290	arg1	glycolipids					1280:1290	two unidentified glycolipids	1263:1290	two unidentified glycolipids	1263:1290	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid.
34904944	4	8	theme	16S	294:296	arg1	similarity					317:326	16S rRNA gene sequence similarity	294:326	16S rRNA gene sequence similarity studies	294:334	Based on 16S rRNA gene sequence similarity studies, strain IMT-300T was shown to belong to the genus Leucobacter and was closely related to the type strain of 'Leucobacter margaritiformis' L1T (97.8%).
34904944	16	9	dep	nov.	1853:1856	arg1	with					1859:1862	with	1859:1862	with	1859:1862	nov., with the type strain IMT-300T (CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T).
34904944	5	10	theme	Leucobacter	527:537	arg1	species					539:545	Leucobacter species	527:545	Leucobacter species	527:545	Similarity to all other type strains of Leucobacter species was lower than 97.2 %.
34904944	4	11	theme	gene	303:306	arg1	similarity					317:326	16S rRNA gene sequence similarity	294:326	16S rRNA gene sequence similarity studies	294:334	Based on 16S rRNA gene sequence similarity studies, strain IMT-300T was shown to belong to the genus Leucobacter and was closely related to the type strain of 'Leucobacter margaritiformis' L1T (97.8%).
34904944	15	12	theme	novel	1775:1779	arg1	species					1793:1799	a novel Leucobacter species	1773:1799	a novel Leucobacter species	1773:1799	Strain IMT-300T represents a novel Leucobacter species, for which we propose the name Leucobacter soli sp.
34904944	14	13	theme	ANI	1563:1565	arg1	analyses					1576:1583	ANI and dDDH analyses	1563:1583	analyses	1576:1583	The results of ANI and dDDH analyses and physiological and biochemical tests allowed a genotypic and phenotypic differentiation of strain IMT-300T from the most closely related Leucobacter species.
34904944	13	14	dep	C15 	1453:1456	arg1	anteiso					1494:1500	 0 anteiso	1491:1500	the major fatty acids C15 : 0 anteiso, C16 : 0 iso and C17 : 0 anteiso	1431:1500	Strain IMT-300T contained the major fatty acids C15 : 0 anteiso, C16 : 0 iso and C17 : 0 anteiso, like other members of the genus Leucobacter.
34904944	13	14	dep	C15 	1453:1456	arg1	iso					1478:1480	 0 iso	1475:1480	 0 iso	1475:1480	Strain IMT-300T contained the major fatty acids C15 : 0 anteiso, C16 : 0 iso and C17 : 0 anteiso, like other members of the genus Leucobacter.
34904944	13	14	dep	C15 	1453:1456	arg1	C16 					1470:1473	C16 	1470:1473	C16 	1470:1473	Strain IMT-300T contained the major fatty acids C15 : 0 anteiso, C16 : 0 iso and C17 : 0 anteiso, like other members of the genus Leucobacter.
34904944	13	14	dep	C15 	1453:1456	arg1	C17 					1486:1489	C17 	1486:1489	C17 	1486:1489	Strain IMT-300T contained the major fatty acids C15 : 0 anteiso, C16 : 0 iso and C17 : 0 anteiso, like other members of the genus Leucobacter.
34904944	7	15	theme	diagnostic	891:900	arg1	acid					910:913	the diagnostic diamino acid	887:913	the diagnostic diamino acid	887:913	The peptidoglycan of strain IMT-300T contained l-2,4-diaminobutyric acid as the diagnostic diamino acid.
34904944	7	15	theme	diagnostic	891:900	arg1	acid					879:882	l-2,4-diaminobutyric acid	858:882	l-2,4-diaminobutyric acid	858:882	The peptidoglycan of strain IMT-300T contained l-2,4-diaminobutyric acid as the diagnostic diamino acid.
34904944	15	16	theme	Strain	1746:1751	arg1	IMT-300T					1753:1760	Strain IMT-300T	1746:1760	Strain IMT-300T	1746:1760	Strain IMT-300T represents a novel Leucobacter species, for which we propose the name Leucobacter soli sp.
34904944	14	17	theme	Leucobacter	1725:1735	arg1	species					1737:1743	the most closely related Leucobacter species	1700:1743	the most closely related Leucobacter species	1700:1743	The results of ANI and dDDH analyses and physiological and biochemical tests allowed a genotypic and phenotypic differentiation of strain IMT-300T from the most closely related Leucobacter species.
34904944	7	18	theme	l-2,4-diaminobutyric	858:877	arg1	acid					910:913	the diagnostic diamino acid	887:913	the diagnostic diamino acid	887:913	The peptidoglycan of strain IMT-300T contained l-2,4-diaminobutyric acid as the diagnostic diamino acid.
34904944	7	18	theme	l-2,4-diaminobutyric	858:877	arg1	acid					879:882	l-2,4-diaminobutyric acid	858:882	l-2,4-diaminobutyric acid	858:882	The peptidoglycan of strain IMT-300T contained l-2,4-diaminobutyric acid as the diagnostic diamino acid.
34904944	14	19	theme	dDDH	1571:1574	arg1	analyses					1576:1583	ANI and dDDH analyses	1563:1583	analyses	1576:1583	The results of ANI and dDDH analyses and physiological and biochemical tests allowed a genotypic and phenotypic differentiation of strain IMT-300T from the most closely related Leucobacter species.
34904944	13	20	theme	fatty	1441:1445	arg1	acids					1447:1451	the major fatty acids	1431:1451	the major fatty acids C15 : 0 anteiso, C16 : 0 iso and C17 : 0 anteiso	1431:1500	Strain IMT-300T contained the major fatty acids C15 : 0 anteiso, C16 : 0 iso and C17 : 0 anteiso, like other members of the genus Leucobacter.
34904944	7	21	theme	IMT-300T	839:846	arg1	peptidoglycan					815:827	The peptidoglycan	811:827	The peptidoglycan of strain IMT-300T	811:846	The peptidoglycan of strain IMT-300T contained l-2,4-diaminobutyric acid as the diagnostic diamino acid.
34904944	15	22	theme	name	1827:1830	arg1	sp					1849:1850	the name Leucobacter soli sp	1823:1850	the name Leucobacter soli sp	1823:1850	Strain IMT-300T represents a novel Leucobacter species, for which we propose the name Leucobacter soli sp.
34904944	8	23	located	found	980:984	arg2	d-					938:939	d-	938:939	d-	938:939	In addition, glycine, d- and l-alanine and d-glutamic acid were found.
34904944	8	23	located	found	980:984	arg2	glycine					929:935	glycine	929:935	glycine	929:935	In addition, glycine, d- and l-alanine and d-glutamic acid were found.
34904944	8	23	located	found	980:984	arg1	addition					919:926	addition	919:926	addition	919:926	In addition, glycine, d- and l-alanine and d-glutamic acid were found.
34904944	8	23	located	found	980:984	arg2	acid					970:973	d-glutamic acid	959:973	d-glutamic acid	959:973	In addition, glycine, d- and l-alanine and d-glutamic acid were found.
34904944	8	23	located	found	980:984	arg2	l-alanine					945:953	l-alanine	945:953	l-alanine	945:953	In addition, glycine, d- and l-alanine and d-glutamic acid were found.
34904944	16	24	theme	strain	1873:1878	arg1	IMT-300T					1880:1887	the type strain IMT-300T	1864:1887	the type strain IMT-300T (CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T)	1864:1934	nov., with the type strain IMT-300T (CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T).
34904944	16	24	theme	strain	1873:1878	arg1	31600T					1928:1933	CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T	1890:1933	CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T	1890:1933	nov., with the type strain IMT-300T (CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T).
34904944	14	25	theme	IMT-300T	1686:1693	arg1	differentiation					1660:1674	a genotypic and phenotypic differentiation	1633:1674	a genotypic and phenotypic differentiation of strain IMT-300T from the most closely related Leucobacter species	1633:1743	The results of ANI and dDDH analyses and physiological and biochemical tests allowed a genotypic and phenotypic differentiation of strain IMT-300T from the most closely related Leucobacter species.
34904944	2	26	dep	Gram-positive	64:76	arg1	non-spore-forming					79:95	non-spore-forming	79:95	non-spore-forming	79:95	A Gram-positive, non-spore-forming actinobacterium (IMT-300T) was isolated from soil amended with humic acid in Malvern, AL, USA.
34904944	6	27	theme	identity	593:600	arg1	values					649:654	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values	570:654	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the IMT-300T genome assembly	570:691	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the IMT-300T genome assembly and those of the closest relative Leucobacter type strain were 81.4 and 23.3 % (Leucobacter chironomi), respectively.
34904944	13	28	contain	contained	1421:1429	arg2	acids					1447:1451	the major fatty acids	1431:1451	the major fatty acids C15 : 0 anteiso, C16 : 0 iso and C17 : 0 anteiso	1431:1500	Strain IMT-300T contained the major fatty acids C15 : 0 anteiso, C16 : 0 iso and C17 : 0 anteiso, like other members of the genus Leucobacter.
34904944	13	28	contain	contained	1421:1429	arg1	IMT-300T					1412:1419	Strain IMT-300T	1405:1419	Strain IMT-300T	1405:1419	Strain IMT-300T contained the major fatty acids C15 : 0 anteiso, C16 : 0 iso and C17 : 0 anteiso, like other members of the genus Leucobacter.
34904944	13	29	theme	Leucobacter	1535:1545	arg1	members					1514:1520	other members	1508:1520	other members of the genus Leucobacter	1508:1545	Strain IMT-300T contained the major fatty acids C15 : 0 anteiso, C16 : 0 iso and C17 : 0 anteiso, like other members of the genus Leucobacter.
34904944	11	30	theme	aminophospholipid	1312:1328	arg1	amounts					1220:1226	minor amounts	1214:1226	minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid	1214:1328	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid.
34904944	11	30	theme	aminophospholipid	1312:1328	arg1	aminophospholipid					1312:1328	aminophospholipid	1312:1328	aminophospholipid	1312:1328	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid.
34904944	11	30	theme	aminophospholipid	1312:1328	arg1	phospholipids					1248:1260	two unidentified phospholipids	1231:1260	two unidentified phospholipids	1231:1260	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid.
34904944	11	30	theme	aminophospholipid	1312:1328	arg1	glycolipids					1280:1290	two unidentified glycolipids	1263:1290	two unidentified glycolipids	1263:1290	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid.
34904944	13	31	theme	Strain	1405:1410	arg1	IMT-300T					1412:1419	Strain IMT-300T	1405:1419	Strain IMT-300T	1405:1419	Strain IMT-300T contained the major fatty acids C15 : 0 anteiso, C16 : 0 iso and C17 : 0 anteiso, like other members of the genus Leucobacter.
34904944	0	32	theme	Leucobacter	0:10	arg1	sp					17:18	Leucobacter soli sp	0:18	Leucobacter soli sp.	0:19	Leucobacter soli sp.
34904944	14	33	theme	strain	1679:1684	arg1	IMT-300T					1686:1693	strain IMT-300T	1679:1693	strain IMT-300T	1679:1693	The results of ANI and dDDH analyses and physiological and biochemical tests allowed a genotypic and phenotypic differentiation of strain IMT-300T from the most closely related Leucobacter species.
34904944	6	34	theme	type	739:742	arg1	strain					744:749	the closest relative Leucobacter type strain	706:749	the closest relative Leucobacter type strain	706:749	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the IMT-300T genome assembly and those of the closest relative Leucobacter type strain were 81.4 and 23.3 % (Leucobacter chironomi), respectively.
34904944	4	35	theme	margaritiformis	457:471	arg1	L1T					474:476	'Leucobacter margaritiformis' L1T	444:476	'Leucobacter margaritiformis' L1T	444:476	Based on 16S rRNA gene sequence similarity studies, strain IMT-300T was shown to belong to the genus Leucobacter and was closely related to the type strain of 'Leucobacter margaritiformis' L1T (97.8%).
34904944	4	36	theme	L1T	474:476	arg1	strain					434:439	the type strain	425:439	the type strain of 'Leucobacter margaritiformis' L1T	425:476	Based on 16S rRNA gene sequence similarity studies, strain IMT-300T was shown to belong to the genus Leucobacter and was closely related to the type strain of 'Leucobacter margaritiformis' L1T (97.8%).
34904944	16	37	theme	CIP	1890:1892	arg1	IMT-300T					1880:1887	the type strain IMT-300T	1864:1887	the type strain IMT-300T (CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T)	1864:1934	nov., with the type strain IMT-300T (CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T).
34904944	16	37	theme	CIP	1890:1892	arg1	31600T					1928:1933	CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T	1890:1933	CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T	1890:1933	nov., with the type strain IMT-300T (CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T).
34904944	16	38	theme	111803T=DSM	1894:1904	arg1	IMT-300T					1880:1887	the type strain IMT-300T	1864:1887	the type strain IMT-300T (CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T)	1864:1934	nov., with the type strain IMT-300T (CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T).
34904944	16	38	theme	111803T=DSM	1894:1904	arg1	31600T					1928:1933	CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T	1890:1933	CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T	1890:1933	nov., with the type strain IMT-300T (CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T).
34904944	6	39	theme	Leucobacter	727:737	arg1	strain					744:749	the closest relative Leucobacter type strain	706:749	the closest relative Leucobacter type strain	706:749	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the IMT-300T genome assembly and those of the closest relative Leucobacter type strain were 81.4 and 23.3 % (Leucobacter chironomi), respectively.
34904944	11	40	theme	polar	1103:1107	arg1	profile					1115:1121	The polar lipid profile	1099:1121	The polar lipid profile	1099:1121	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid.
34904944	9	41	theme	peptidoglycan	991:1003	arg1	type					1005:1008	The peptidoglycan type	987:1008	The peptidoglycan type	987:1008	The peptidoglycan type represents a variant of B2δ (B11).
34904944	6	42	theme	closest	710:716	arg1	strain					744:749	the closest relative Leucobacter type strain	706:749	the closest relative Leucobacter type strain	706:749	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the IMT-300T genome assembly and those of the closest relative Leucobacter type strain were 81.4 and 23.3 % (Leucobacter chironomi), respectively.
34904944	15	43	theme	soli	1844:1847	arg1	sp					1849:1850	the name Leucobacter soli sp	1823:1850	the name Leucobacter soli sp	1823:1850	Strain IMT-300T represents a novel Leucobacter species, for which we propose the name Leucobacter soli sp.
34904944	6	44	theme	DNA-DNA	620:626	arg1	hybridization					628:640	digital DNA-DNA hybridization	612:640	digital DNA-DNA hybridization (dDDH)	612:647	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the IMT-300T genome assembly and those of the closest relative Leucobacter type strain were 81.4 and 23.3 % (Leucobacter chironomi), respectively.
34904944	6	44	theme	DNA-DNA	620:626	arg1	dDDH					643:646	dDDH	643:646	dDDH	643:646	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the IMT-300T genome assembly and those of the closest relative Leucobacter type strain were 81.4 and 23.3 % (Leucobacter chironomi), respectively.
34904944	14	45	theme	tests	1619:1623	arg1	results					1552:1558	The results	1548:1558	The results of ANI and dDDH analyses and physiological and biochemical tests	1548:1623	The results of ANI and dDDH analyses and physiological and biochemical tests allowed a genotypic and phenotypic differentiation of strain IMT-300T from the most closely related Leucobacter species.
34904944	7	46	contain	contained	848:856	arg1	peptidoglycan					815:827	The peptidoglycan	811:827	The peptidoglycan of strain IMT-300T	811:846	The peptidoglycan of strain IMT-300T contained l-2,4-diaminobutyric acid as the diagnostic diamino acid.
34904944	7	46	contain	contained	848:856	arg2	acid					879:882	l-2,4-diaminobutyric acid	858:882	l-2,4-diaminobutyric acid	858:882	The peptidoglycan of strain IMT-300T contained l-2,4-diaminobutyric acid as the diagnostic diamino acid.
34904944	7	46	contain	contained	848:856	arg2	acid					910:913	the diagnostic diamino acid	887:913	the diagnostic diamino acid	887:913	The peptidoglycan of strain IMT-300T contained l-2,4-diaminobutyric acid as the diagnostic diamino acid.
34904944	11	47	theme	unidentified	1235:1246	arg1	phospholipids					1248:1260	two unidentified phospholipids	1231:1260	two unidentified phospholipids	1231:1260	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid.
34904944	5	48	theme	species	539:545	arg1	strains					516:522	all other type strains	501:522	all other type strains of Leucobacter species	501:545	Similarity to all other type strains of Leucobacter species was lower than 97.2 %.
34904944	9	49	theme	B2δ	1034:1036	arg1	variant					1023:1029	a variant	1021:1029	a variant of B2δ (B11)	1021:1042	The peptidoglycan type represents a variant of B2δ (B11).
34904944	16	50	theme	110505T=CCM	1906:1916	arg1	IMT-300T					1880:1887	the type strain IMT-300T	1864:1887	the type strain IMT-300T (CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T)	1864:1934	nov., with the type strain IMT-300T (CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T).
34904944	16	50	theme	110505T=CCM	1906:1916	arg1	31600T					1928:1933	CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T	1890:1933	CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T	1890:1933	nov., with the type strain IMT-300T (CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T).
34904944	12	51	theme	major	1363:1367	arg1	spermidine					1380:1389	spermidine	1380:1389	spermidine	1380:1389	The polyamine pattern contained major amounts of spermidine and spermine.
34904944	12	51	theme	major	1363:1367	arg1	amounts					1369:1375	major amounts	1363:1375	major amounts of spermidine and spermine	1363:1402	The polyamine pattern contained major amounts of spermidine and spermine.
34904944	12	51	theme	major	1363:1367	arg1	spermine					1395:1402	spermine	1395:1402	spermine	1395:1402	The polyamine pattern contained major amounts of spermidine and spermine.
34904944	10	52	theme	major	1049:1053	arg1	menaquinones					1069:1080	menaquinones MK-10 and MK-11	1069:1096	menaquinones MK-10 and MK-11	1069:1096	The major quinones were menaquinones MK-10 and MK-11.
34904944	10	52	theme	major	1049:1053	arg1	quinones					1055:1062	The major quinones	1045:1062	The major quinones	1045:1062	The major quinones were menaquinones MK-10 and MK-11.
34904944	2	53	from	acid	166:169	arg1	USA					187:189	USA	187:189	USA	187:189	A Gram-positive, non-spore-forming actinobacterium (IMT-300T) was isolated from soil amended with humic acid in Malvern, AL, USA.
34904944	2	53	from	acid	166:169	arg1	Malvern					174:180	Malvern	174:180	Malvern	174:180	A Gram-positive, non-spore-forming actinobacterium (IMT-300T) was isolated from soil amended with humic acid in Malvern, AL, USA.
34904944	4	54	theme	strain	337:342	arg1	IMT-300T					344:351	strain IMT-300T	337:351	strain IMT-300T	337:351	Based on 16S rRNA gene sequence similarity studies, strain IMT-300T was shown to belong to the genus Leucobacter and was closely related to the type strain of 'Leucobacter margaritiformis' L1T (97.8%).
34904944	11	55	theme	unidentified	1267:1278	arg1	glycolipids					1280:1290	two unidentified glycolipids	1263:1290	two unidentified glycolipids	1263:1290	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid.
34904944	5	56	theme	type	511:514	arg1	strains					516:522	all other type strains	501:522	all other type strains of Leucobacter species	501:545	Similarity to all other type strains of Leucobacter species was lower than 97.2 %.
34904944	6	57	theme	IMT-300T	668:675	arg1	assembly					684:691	the IMT-300T genome assembly	664:691	the IMT-300T genome assembly	664:691	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the IMT-300T genome assembly and those of the closest relative Leucobacter type strain were 81.4 and 23.3 % (Leucobacter chironomi), respectively.
34904944	1	58	from	soil	32:35	arg1	nov.					21:24	nov.	21:24	nov.	21:24	nov., from soil amended with humic acid.
34904944	4	59	theme	rRNA	298:301	arg1	similarity					317:326	16S rRNA gene sequence similarity	294:326	16S rRNA gene sequence similarity studies	294:334	Based on 16S rRNA gene sequence similarity studies, strain IMT-300T was shown to belong to the genus Leucobacter and was closely related to the type strain of 'Leucobacter margaritiformis' L1T (97.8%).
34904944	15	60	theme	Leucobacter	1781:1791	arg1	species					1793:1799	a novel Leucobacter species	1773:1799	a novel Leucobacter species	1773:1799	Strain IMT-300T represents a novel Leucobacter species, for which we propose the name Leucobacter soli sp.
34904944	11	61	theme	lipid	1109:1113	arg1	profile					1115:1121	The polar lipid profile	1099:1121	The polar lipid profile	1099:1121	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid.
34904944	3	62	used	used	211:214	arg2	soil					197:200	This soil	192:200	This soil	192:200	This soil has been used for 50+years for the cultivation of earthworms for use as fish bait.
34904944	4	63	theme	sequence	308:315	arg1	similarity					317:326	16S rRNA gene sequence similarity	294:326	16S rRNA gene sequence similarity studies	294:334	Based on 16S rRNA gene sequence similarity studies, strain IMT-300T was shown to belong to the genus Leucobacter and was closely related to the type strain of 'Leucobacter margaritiformis' L1T (97.8%).
34904944	4	64	theme	genus	380:384	arg1	Leucobacter					386:396	the genus Leucobacter	376:396	the genus Leucobacter	376:396	Based on 16S rRNA gene sequence similarity studies, strain IMT-300T was shown to belong to the genus Leucobacter and was closely related to the type strain of 'Leucobacter margaritiformis' L1T (97.8%).
34904944	11	65	theme	minor	1214:1218	arg1	amounts					1220:1226	minor amounts	1214:1226	minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid	1214:1328	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid.
34904944	11	65	theme	minor	1214:1218	arg1	aminophospholipid					1312:1328	aminophospholipid	1312:1328	aminophospholipid	1312:1328	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid.
34904944	11	65	theme	minor	1214:1218	arg1	phospholipids					1248:1260	two unidentified phospholipids	1231:1260	two unidentified phospholipids	1231:1260	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid.
34904944	11	65	theme	minor	1214:1218	arg1	glycolipids					1280:1290	two unidentified glycolipids	1263:1290	two unidentified glycolipids	1263:1290	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid.
34904944	2	66	theme	Gram-positive	64:76	arg1	IMT-300T					114:121	IMT-300T	114:121	IMT-300T	114:121	A Gram-positive, non-spore-forming actinobacterium (IMT-300T) was isolated from soil amended with humic acid in Malvern, AL, USA.
34904944	2	66	theme	Gram-positive	64:76	arg1	actinobacterium					97:111	A Gram-positive, non-spore-forming actinobacterium	62:111	A Gram-positive, non-spore-forming actinobacterium (IMT-300T)	62:122	A Gram-positive, non-spore-forming actinobacterium (IMT-300T) was isolated from soil amended with humic acid in Malvern, AL, USA.
34904944	7	67	theme	diamino	902:908	arg1	acid					910:913	the diagnostic diamino acid	887:913	the diagnostic diamino acid	887:913	The peptidoglycan of strain IMT-300T contained l-2,4-diaminobutyric acid as the diagnostic diamino acid.
34904944	7	67	theme	diamino	902:908	arg1	acid					879:882	l-2,4-diaminobutyric acid	858:882	l-2,4-diaminobutyric acid	858:882	The peptidoglycan of strain IMT-300T contained l-2,4-diaminobutyric acid as the diagnostic diamino acid.
34904944	3	68	theme	earthworms	252:261	arg1	cultivation					237:247	the cultivation	233:247	the cultivation of earthworms for use as fish bait	233:282	This soil has been used for 50+years for the cultivation of earthworms for use as fish bait.
34904944	14	69	theme	related	1717:1723	arg1	species					1737:1743	the most closely related Leucobacter species	1700:1743	the most closely related Leucobacter species	1700:1743	The results of ANI and dDDH analyses and physiological and biochemical tests allowed a genotypic and phenotypic differentiation of strain IMT-300T from the most closely related Leucobacter species.
34904944	13	70	theme	major	1435:1439	arg1	acids					1447:1451	the major fatty acids	1431:1451	the major fatty acids C15 : 0 anteiso, C16 : 0 iso and C17 : 0 anteiso	1431:1500	Strain IMT-300T contained the major fatty acids C15 : 0 anteiso, C16 : 0 iso and C17 : 0 anteiso, like other members of the genus Leucobacter.
34904944	3	71	theme	fish	274:277	arg1	bait					279:282	fish bait	274:282	fish bait	274:282	This soil has been used for 50+years for the cultivation of earthworms for use as fish bait.
34904944	4	72	theme	type	429:432	arg1	strain					434:439	the type strain	425:439	the type strain of 'Leucobacter margaritiformis' L1T	425:476	Based on 16S rRNA gene sequence similarity studies, strain IMT-300T was shown to belong to the genus Leucobacter and was closely related to the type strain of 'Leucobacter margaritiformis' L1T (97.8%).
34904944	15	73	theme	Leucobacter	1832:1842	arg1	sp					1849:1850	the name Leucobacter soli sp	1823:1850	the name Leucobacter soli sp	1823:1850	Strain IMT-300T represents a novel Leucobacter species, for which we propose the name Leucobacter soli sp.
34904944	7	74	theme	strain	832:837	arg1	IMT-300T					839:846	strain IMT-300T	832:846	strain IMT-300T	832:846	The peptidoglycan of strain IMT-300T contained l-2,4-diaminobutyric acid as the diagnostic diamino acid.
34904944	5	75	theme	other	505:509	arg1	strains					516:522	all other type strains	501:522	all other type strains of Leucobacter species	501:545	Similarity to all other type strains of Leucobacter species was lower than 97.2 %.
34904944	1	76	theme	humic	50:54	arg1	acid					56:59	humic acid	50:59	humic acid	50:59	nov., from soil amended with humic acid.
34904944	13	77	theme	genus	1529:1533	arg1	Leucobacter					1535:1545	the genus Leucobacter	1525:1545	the genus Leucobacter	1525:1545	Strain IMT-300T contained the major fatty acids C15 : 0 anteiso, C16 : 0 iso and C17 : 0 anteiso, like other members of the genus Leucobacter.
34904944	6	78	theme	nucleotide	582:591	arg1	ANI					603:605	ANI	603:605	ANI	603:605	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the IMT-300T genome assembly and those of the closest relative Leucobacter type strain were 81.4 and 23.3 % (Leucobacter chironomi), respectively.
34904944	6	78	theme	nucleotide	582:591	arg1	identity					593:600	The average nucleotide identity	570:600	The average nucleotide identity (ANI)	570:606	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the IMT-300T genome assembly and those of the closest relative Leucobacter type strain were 81.4 and 23.3 % (Leucobacter chironomi), respectively.
34904944	0	79	theme	soli	12:15	arg1	sp					17:18	Leucobacter soli sp	0:18	Leucobacter soli sp.	0:19	Leucobacter soli sp.
34904944	10	80	dep	menaquinones	1069:1080	arg1	menaquinones					1069:1080	menaquinones MK-10 and MK-11	1069:1096	menaquinones MK-10 and MK-11	1069:1096	The major quinones were menaquinones MK-10 and MK-11.
34904944	10	80	dep	menaquinones	1069:1080	arg1	MK-11					1092:1096	MK-11	1092:1096	MK-11	1092:1096	The major quinones were menaquinones MK-10 and MK-11.
34904944	10	80	dep	menaquinones	1069:1080	arg1	MK-10					1082:1086	MK-10	1082:1086	MK-10	1082:1086	The major quinones were menaquinones MK-10 and MK-11.
34904944	13	81	dep	acids	1447:1451	arg1	C15 					1453:1456	C15 	1453:1456	the major fatty acids C15 : 0 anteiso, C16 : 0 iso and C17 : 0 anteiso	1431:1500	Strain IMT-300T contained the major fatty acids C15 : 0 anteiso, C16 : 0 iso and C17 : 0 anteiso, like other members of the genus Leucobacter.
34904944	12	82	contain	contained	1353:1361	arg1	pattern					1345:1351	The polyamine pattern	1331:1351	The polyamine pattern	1331:1351	The polyamine pattern contained major amounts of spermidine and spermine.
34904944	12	82	contain	contained	1353:1361	arg2	spermidine					1380:1389	spermidine	1380:1389	spermidine	1380:1389	The polyamine pattern contained major amounts of spermidine and spermine.
34904944	12	82	contain	contained	1353:1361	arg2	spermine					1395:1402	spermine	1395:1402	spermine	1395:1402	The polyamine pattern contained major amounts of spermidine and spermine.
34904944	12	82	contain	contained	1353:1361	arg2	amounts					1369:1375	major amounts	1363:1375	major amounts of spermidine and spermine	1363:1402	The polyamine pattern contained major amounts of spermidine and spermine.
34904944	16	83	theme	type	1868:1871	arg1	IMT-300T					1880:1887	the type strain IMT-300T	1864:1887	the type strain IMT-300T (CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T)	1864:1934	nov., with the type strain IMT-300T (CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T).
34904944	16	83	theme	type	1868:1871	arg1	31600T					1928:1933	CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T	1890:1933	CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T	1890:1933	nov., with the type strain IMT-300T (CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T).
34904944	13	84	theme	other	1508:1512	arg1	members					1514:1520	other members	1508:1520	other members of the genus Leucobacter	1508:1545	Strain IMT-300T contained the major fatty acids C15 : 0 anteiso, C16 : 0 iso and C17 : 0 anteiso, like other members of the genus Leucobacter.
34904944	2	85	theme	humic	160:164	arg1	acid					166:169	humic acid	160:169	humic acid in Malvern, AL, USA	160:189	A Gram-positive, non-spore-forming actinobacterium (IMT-300T) was isolated from soil amended with humic acid in Malvern, AL, USA.
34904944	14	86	theme	phenotypic	1649:1658	arg1	differentiation					1660:1674	a genotypic and phenotypic differentiation	1633:1674	a genotypic and phenotypic differentiation of strain IMT-300T from the most closely related Leucobacter species	1633:1743	The results of ANI and dDDH analyses and physiological and biochemical tests allowed a genotypic and phenotypic differentiation of strain IMT-300T from the most closely related Leucobacter species.
34904944	8	87	theme	d-glutamic	959:968	arg1	acid					970:973	d-glutamic acid	959:973	d-glutamic acid	959:973	In addition, glycine, d- and l-alanine and d-glutamic acid were found.
34904944	12	88	theme	spermine	1395:1402	arg1	spermidine					1380:1389	spermidine	1380:1389	spermidine	1380:1389	The polyamine pattern contained major amounts of spermidine and spermine.
34904944	12	88	theme	spermine	1395:1402	arg1	amounts					1369:1375	major amounts	1363:1375	major amounts of spermidine and spermine	1363:1402	The polyamine pattern contained major amounts of spermidine and spermine.
34904944	12	88	theme	spermine	1395:1402	arg1	spermine					1395:1402	spermine	1395:1402	spermine	1395:1402	The polyamine pattern contained major amounts of spermidine and spermine.
34904944	11	89	dep	lipids	1146:1151	arg1	moderate					1202:1209	moderate	1202:1209	moderate	1202:1209	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid.
34904944	11	89	dep	lipids	1146:1151	arg1	lipids					1146:1151	the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid	1136:1328	the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid	1136:1328	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid.
34904944	11	89	dep	lipids	1146:1151	arg1	phosphatidylglycerol					1177:1196	phosphatidylglycerol	1177:1196	phosphatidylglycerol	1177:1196	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid.
34904944	11	89	dep	lipids	1146:1151	arg1	diphosphatidylglycerol					1153:1174	diphosphatidylglycerol	1153:1174	diphosphatidylglycerol	1153:1174	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol and moderate to minor amounts of two unidentified phospholipids, two unidentified glycolipids and an unidentified aminophospholipid.
34904944	14	90	theme	genotypic	1635:1643	arg1	differentiation					1660:1674	a genotypic and phenotypic differentiation	1633:1674	a genotypic and phenotypic differentiation of strain IMT-300T from the most closely related Leucobacter species	1633:1743	The results of ANI and dDDH analyses and physiological and biochemical tests allowed a genotypic and phenotypic differentiation of strain IMT-300T from the most closely related Leucobacter species.
34904944	6	91	theme	relative	718:725	arg1	strain					744:749	the closest relative Leucobacter type strain	706:749	the closest relative Leucobacter type strain	706:749	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the IMT-300T genome assembly and those of the closest relative Leucobacter type strain were 81.4 and 23.3 % (Leucobacter chironomi), respectively.
34904944	2	92	attach	isolated	128:135	arg1	soil					142:145	soil	142:145	soil amended with humic acid in Malvern, AL, USA	142:189	A Gram-positive, non-spore-forming actinobacterium (IMT-300T) was isolated from soil amended with humic acid in Malvern, AL, USA.
34904944	2	92	attach	isolated	128:135	arg2	IMT-300T					114:121	IMT-300T	114:121	IMT-300T	114:121	A Gram-positive, non-spore-forming actinobacterium (IMT-300T) was isolated from soil amended with humic acid in Malvern, AL, USA.
34904944	2	92	attach	isolated	128:135	arg2	actinobacterium					97:111	A Gram-positive, non-spore-forming actinobacterium	62:111	A Gram-positive, non-spore-forming actinobacterium (IMT-300T)	62:122	A Gram-positive, non-spore-forming actinobacterium (IMT-300T) was isolated from soil amended with humic acid in Malvern, AL, USA.
34904944	4	93	theme	Leucobacter	445:455	arg1	L1T					474:476	'Leucobacter margaritiformis' L1T	444:476	'Leucobacter margaritiformis' L1T	444:476	Based on 16S rRNA gene sequence similarity studies, strain IMT-300T was shown to belong to the genus Leucobacter and was closely related to the type strain of 'Leucobacter margaritiformis' L1T (97.8%).
34904944	13	94	theme	 0	1491:1492	arg1	anteiso					1494:1500	 0 anteiso	1491:1500	the major fatty acids C15 : 0 anteiso, C16 : 0 iso and C17 : 0 anteiso	1431:1500	Strain IMT-300T contained the major fatty acids C15 : 0 anteiso, C16 : 0 iso and C17 : 0 anteiso, like other members of the genus Leucobacter.
34904944	6	95	theme	hybridization	628:640	arg1	values					649:654	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values	570:654	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the IMT-300T genome assembly	570:691	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the IMT-300T genome assembly and those of the closest relative Leucobacter type strain were 81.4 and 23.3 % (Leucobacter chironomi), respectively.
34904944	4	96	dep	related	414:420	arg1	%					483:483	97.8%	479:483	97.8%	479:483	Based on 16S rRNA gene sequence similarity studies, strain IMT-300T was shown to belong to the genus Leucobacter and was closely related to the type strain of 'Leucobacter margaritiformis' L1T (97.8%).
34904944	16	97	theme	9020T=LMG	1918:1926	arg1	IMT-300T					1880:1887	the type strain IMT-300T	1864:1887	the type strain IMT-300T (CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T)	1864:1934	nov., with the type strain IMT-300T (CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T).
34904944	16	97	theme	9020T=LMG	1918:1926	arg1	31600T					1928:1933	CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T	1890:1933	CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T	1890:1933	nov., with the type strain IMT-300T (CIP 111803T=DSM 110505T=CCM 9020T=LMG 31600T).
34904944	6	98	theme	digital	612:618	arg1	hybridization					628:640	digital DNA-DNA hybridization	612:640	digital DNA-DNA hybridization (dDDH)	612:647	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the IMT-300T genome assembly and those of the closest relative Leucobacter type strain were 81.4 and 23.3 % (Leucobacter chironomi), respectively.
34904944	6	98	theme	digital	612:618	arg1	dDDH					643:646	dDDH	643:646	dDDH	643:646	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the IMT-300T genome assembly and those of the closest relative Leucobacter type strain were 81.4 and 23.3 % (Leucobacter chironomi), respectively.
34904944	14	99	from	species	1737:1743	arg1	differentiation					1660:1674	a genotypic and phenotypic differentiation	1633:1674	a genotypic and phenotypic differentiation of strain IMT-300T from the most closely related Leucobacter species	1633:1743	The results of ANI and dDDH analyses and physiological and biochemical tests allowed a genotypic and phenotypic differentiation of strain IMT-300T from the most closely related Leucobacter species.
34904944	14	100	theme	biochemical	1607:1617	arg1	tests					1619:1623	physiological and biochemical tests	1589:1623	physiological and biochemical tests	1589:1623	The results of ANI and dDDH analyses and physiological and biochemical tests allowed a genotypic and phenotypic differentiation of strain IMT-300T from the most closely related Leucobacter species.
34904944	12	101	theme	spermidine	1380:1389	arg1	spermidine					1380:1389	spermidine	1380:1389	spermidine	1380:1389	The polyamine pattern contained major amounts of spermidine and spermine.
34904944	12	101	theme	spermidine	1380:1389	arg1	amounts					1369:1375	major amounts	1363:1375	major amounts of spermidine and spermine	1363:1402	The polyamine pattern contained major amounts of spermidine and spermine.
34904944	12	101	theme	spermidine	1380:1389	arg1	spermine					1395:1402	spermine	1395:1402	spermine	1395:1402	The polyamine pattern contained major amounts of spermidine and spermine.
34904944	14	102	theme	physiological	1589:1601	arg1	tests					1619:1623	physiological and biochemical tests	1589:1623	physiological and biochemical tests	1589:1623	The results of ANI and dDDH analyses and physiological and biochemical tests allowed a genotypic and phenotypic differentiation of strain IMT-300T from the most closely related Leucobacter species.
34699119	6	0	theme	granulocyte	805:815	arg1	GMCSF					855:859	GMCSF	855:859	GMCSF	855:859	CVS also inhibited secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum.
34699119	6	0	theme	granulocyte	805:815	arg1	factor					847:852	granulocyte macrophage colony-stimulating factor	805:852	granulocyte macrophage colony-stimulating factor (GMCSF)	805:860	CVS also inhibited secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum.
34699119	5	1	theme	Tnf	612:614	arg1	Interleukin					661:671	Interleukin	661:671	Interleukin(Il-23)	661:678	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	5	1	theme	Tnf	612:614	arg1	synthase					644:651	inducible nitric oxide synthase	621:651	inducible nitric oxide synthase (Inos)	621:658	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	5	1	theme	Tnf	612:614	arg1	-a					617:618	tumor necrosis factor (Tnf )-a	589:618	tumor necrosis factor (Tnf )-a	589:618	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	5	2	theme	-a	617:618	arg1	expression					575:584	decreased gene expression	560:584	decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues	560:695	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	5	3	theme	tumor	589:593	arg1	Interleukin					661:671	Interleukin	661:671	Interleukin(Il-23)	661:678	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	5	3	theme	tumor	589:593	arg1	synthase					644:651	inducible nitric oxide synthase	621:651	inducible nitric oxide synthase (Inos)	621:658	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	5	3	theme	tumor	589:593	arg1	-a					617:618	tumor necrosis factor (Tnf )-a	589:618	tumor necrosis factor (Tnf )-a	589:618	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	6	4	theme	stimulating	777:787	arg1	G-CSF					797:801	G-CSF	797:801	G-CSF	797:801	CVS also inhibited secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum.
34699119	6	4	theme	stimulating	777:787	arg1	factor					789:794	Granulocyte colony stimulating factor	758:794	Granulocyte colony stimulating factor (G-CSF)	758:802	CVS also inhibited secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum.
34699119	9	5	theme	acids	1489:1493	arg1	regulation					1460:1469	the regulation	1456:1469	the regulation of steroid, fatty acids, and bile acid biosynthesis	1456:1521	These changes may be related to the regulation of steroid, fatty acids, and bile acid biosynthesis.
34699119	5	6	theme	factor	604:609	arg1	Interleukin					661:671	Interleukin	661:671	Interleukin(Il-23)	661:678	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	5	6	theme	factor	604:609	arg1	synthase					644:651	inducible nitric oxide synthase	621:651	inducible nitric oxide synthase (Inos)	621:658	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	5	6	theme	factor	604:609	arg1	-a					617:618	tumor necrosis factor (Tnf )-a	589:618	tumor necrosis factor (Tnf )-a	589:618	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	10	7	theme	Il-10-/-	1614:1621	arg1	mice					1623:1626	Il-10-/- mice	1614:1626	Il-10-/- mice	1614:1626	CONCLUSION This study demonstrated that CVS ameliorates spontaneous ulcerative colitis in Il-10-/- mice, which suggests CVS supplementation may serve as a protective dietary nutrient against colitis.
34699119	7	8	theme	Il-10-/-	1208:1215	arg1	mice					1217:1220	Il-10-/- mice	1208:1220	Il-10-/- mice	1208:1220	While CVS enhanced Regenerating Family Member 3 Gamma (Reg3γ), Mucin (Muc2, Muc3, and Muc4 gene expression, promoted intestinal epithelial cells to secrete Muc-2, and increased the content of acetic acid in intestinal tract of Il-10-/- mice.
34699119	8	9	theme	Alistipes	1330:1338	arg1	abundance					1304:1312	the abundance	1300:1312	the abundance of Akkermansia, Alistipes, and Lactobacillus	1300:1357	Additionally, CVS altered the composition of the gut microbiota by promoting the abundance of Akkermansia, Alistipes, and Lactobacillus, while inhibiting Desulfovibrio and Clostridium sensu stricto 1.
34699119	3	10	from	colitis	342:348	arg1	mice					362:365	Il-10-/- mice	353:365	Il-10-/- mice	353:365	This study evaluates the effects of CVS on spontaneous colitis in Il-10-/- mice.
34699119	10	11	theme	spontaneous	1580:1590	arg1	colitis					1603:1609	spontaneous ulcerative colitis	1580:1609	spontaneous ulcerative colitis	1580:1609	CONCLUSION This study demonstrated that CVS ameliorates spontaneous ulcerative colitis in Il-10-/- mice, which suggests CVS supplementation may serve as a protective dietary nutrient against colitis.
34699119	2	12	contain	has	193:195	arg2	functions					205:213	various functions	197:213	various functions such as anti-inflammatory, anti-tumor, hypoglycemic, and hypolipidemic	197:284	It has various functions such as anti-inflammatory, anti-tumor, hypoglycemic, and hypolipidemic.
34699119	2	12	contain	has	193:195	arg2	anti-tumor					242:251	anti-tumor	242:251	anti-tumor	242:251	It has various functions such as anti-inflammatory, anti-tumor, hypoglycemic, and hypolipidemic.
34699119	2	12	contain	has	193:195	arg2	hypoglycemic					254:265	hypoglycemic	254:265	hypoglycemic	254:265	It has various functions such as anti-inflammatory, anti-tumor, hypoglycemic, and hypolipidemic.
34699119	2	12	contain	has	193:195	arg1	It					190:191	It	190:191	It	190:191	It has various functions such as anti-inflammatory, anti-tumor, hypoglycemic, and hypolipidemic.
34699119	2	12	contain	has	193:195	arg2	hypolipidemic					272:284	hypolipidemic	272:284	hypolipidemic	272:284	It has various functions such as anti-inflammatory, anti-tumor, hypoglycemic, and hypolipidemic.
34699119	2	12	contain	has	193:195	arg2	anti-inflammatory					223:239	anti-inflammatory	223:239	anti-inflammatory	223:239	It has various functions such as anti-inflammatory, anti-tumor, hypoglycemic, and hypolipidemic.
34699119	9	13	theme	acid	1505:1508	arg1	biosynthesis					1510:1521	bile acid biosynthesis	1500:1521	bile acid biosynthesis	1500:1521	These changes may be related to the regulation of steroid, fatty acids, and bile acid biosynthesis.
34699119	5	14	theme	inducible	621:629	arg1	Inos					654:657	Inos	654:657	Inos	654:657	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	5	14	theme	inducible	621:629	arg1	synthase					644:651	inducible nitric oxide synthase	621:651	inducible nitric oxide synthase (Inos)	621:658	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	5	14	theme	inducible	621:629	arg1	-a					617:618	tumor necrosis factor (Tnf )-a	589:618	tumor necrosis factor (Tnf )-a	589:618	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	8	15	theme	microbiota	1276:1285	arg1	composition					1253:1263	the composition	1249:1263	the composition of the gut microbiota	1249:1285	Additionally, CVS altered the composition of the gut microbiota by promoting the abundance of Akkermansia, Alistipes, and Lactobacillus, while inhibiting Desulfovibrio and Clostridium sensu stricto 1.
34699119	6	16	theme	colony-stimulating	828:845	arg1	GMCSF					855:859	GMCSF	855:859	GMCSF	855:859	CVS also inhibited secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum.
34699119	6	16	theme	colony-stimulating	828:845	arg1	factor					847:852	granulocyte macrophage colony-stimulating factor	805:852	granulocyte macrophage colony-stimulating factor (GMCSF)	805:860	CVS also inhibited secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum.
34699119	4	17	theme	body	402:405	arg1	weight					407:412	1 g kg-1 body weight	393:412	1 g kg-1 body weight	393:412	METHODS AND RESULTS CVS (1 g kg-1 body weight) is administered to mice for 42 days.
34699119	4	17	theme	body	402:405	arg1	CVS					388:390	METHODS AND RESULTS CVS	368:390	METHODS AND RESULTS CVS (1 g kg-1 body weight)	368:413	METHODS AND RESULTS CVS (1 g kg-1 body weight) is administered to mice for 42 days.
34699119	7	18	theme	acetic	1173:1178	arg1	acid					1180:1183	acetic acid	1173:1183	acetic acid in intestinal tract of Il-10-/- mice	1173:1220	While CVS enhanced Regenerating Family Member 3 Gamma (Reg3γ), Mucin (Muc2, Muc3, and Muc4 gene expression, promoted intestinal epithelial cells to secrete Muc-2, and increased the content of acetic acid in intestinal tract of Il-10-/- mice.
34699119	5	19	theme	oxide	638:642	arg1	Inos					654:657	Inos	654:657	Inos	654:657	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	5	19	theme	oxide	638:642	arg1	synthase					644:651	inducible nitric oxide synthase	621:651	inducible nitric oxide synthase (Inos)	621:658	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	5	19	theme	oxide	638:642	arg1	-a					617:618	tumor necrosis factor (Tnf )-a	589:618	tumor necrosis factor (Tnf )-a	589:618	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	0	20	theme	Il-10	69:73	arg1	Mice					85:88	Il-10 Deficient Mice	69:88	Il-10 Deficient Mice	69:88	Cereal Vinegar Sediment Alleviates Spontaneous Ulcerative Colitis in Il-10 Deficient Mice.
34699119	5	21	from	expression	575:584	arg1	tissues					689:695	colon tissues	683:695	colon tissues	683:695	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	6	22	theme	-γ	879:880	arg1	secretion					726:734	secretion	726:734	secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum	726:978	CVS also inhibited secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum.
34699119	3	23	theme	Il-10-/-	353:360	arg1	mice					362:365	Il-10-/- mice	353:365	Il-10-/- mice	353:365	This study evaluates the effects of CVS on spontaneous colitis in Il-10-/- mice.
34699119	8	24	theme	Akkermansia	1317:1327	arg1	abundance					1304:1312	the abundance	1300:1312	the abundance of Akkermansia, Alistipes, and Lactobacillus	1300:1357	Additionally, CVS altered the composition of the gut microbiota by promoting the abundance of Akkermansia, Alistipes, and Lactobacillus, while inhibiting Desulfovibrio and Clostridium sensu stricto 1.
34699119	10	25	dep	CONCLUSION	1524:1533	arg1	demonstrated					1546:1557	demonstrated	1546:1557	demonstrated that CVS ameliorates spontaneous ulcerative colitis in Il-10-/- mice, which suggests CVS supplementation may serve as a protective dietary nutrient against colitis	1546:1721	CONCLUSION This study demonstrated that CVS ameliorates spontaneous ulcerative colitis in Il-10-/- mice, which suggests CVS supplementation may serve as a protective dietary nutrient against colitis.
34699119	5	26	theme	gene	570:573	arg1	expression					575:584	decreased gene expression	560:584	decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues	560:695	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	7	27	from	content	1162:1168	arg1	tract					1199:1203	intestinal tract	1188:1203	intestinal tract of Il-10-/- mice	1188:1220	While CVS enhanced Regenerating Family Member 3 Gamma (Reg3γ), Mucin (Muc2, Muc3, and Muc4 gene expression, promoted intestinal epithelial cells to secrete Muc-2, and increased the content of acetic acid in intestinal tract of Il-10-/- mice.
34699119	0	28	from	Colitis	58:64	arg1	Mice					85:88	Il-10 Deficient Mice	69:88	Il-10 Deficient Mice	69:88	Cereal Vinegar Sediment Alleviates Spontaneous Ulcerative Colitis in Il-10 Deficient Mice.
34699119	6	29	dep	Cell	923:926	arg1	Expressed					928:936	Expressed	928:936	Expressed	928:936	CVS also inhibited secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum.
34699119	6	29	dep	Cell	923:926	arg1	Secreted					953:960	Secreted	953:960	Presumably Secreted (RANTES) in serum	942:978	CVS also inhibited secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum.
34699119	0	30	theme	Ulcerative	47:56	arg1	Colitis					58:64	Spontaneous Ulcerative Colitis	35:64	Spontaneous Ulcerative Colitis in Il-10 Deficient Mice	35:88	Cereal Vinegar Sediment Alleviates Spontaneous Ulcerative Colitis in Il-10 Deficient Mice.
34699119	0	31	theme	Cereal	0:5	arg1	Sediment					15:22	Cereal Vinegar Sediment	0:22	Cereal Vinegar Sediment	0:22	Cereal Vinegar Sediment Alleviates Spontaneous Ulcerative Colitis in Il-10 Deficient Mice.
34699119	1	32	theme	Cereal	97:102	arg1	CVS					122:124	CVS	122:124	CVS	122:124	SCOPE Cereal vinegar sediment (CVS) is precipitation generated during the preservation of vinegar.
34699119	1	32	theme	Cereal	97:102	arg1	sediment					112:119	SCOPE Cereal vinegar sediment	91:119	SCOPE Cereal vinegar sediment (CVS)	91:125	SCOPE Cereal vinegar sediment (CVS) is precipitation generated during the preservation of vinegar.
34699119	7	33	dep	Muc2	1051:1054	arg1	expression					1077:1086	gene expression	1072:1086	gene expression	1072:1086	While CVS enhanced Regenerating Family Member 3 Gamma (Reg3γ), Mucin (Muc2, Muc3, and Muc4 gene expression, promoted intestinal epithelial cells to secrete Muc-2, and increased the content of acetic acid in intestinal tract of Il-10-/- mice.
34699119	6	34	dep	Secreted	953:960	arg1	RANTES					963:968	RANTES	963:968	RANTES	963:968	CVS also inhibited secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum.
34699119	6	35	theme	colony	770:775	arg1	G-CSF					797:801	G-CSF	797:801	G-CSF	797:801	CVS also inhibited secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum.
34699119	6	35	theme	colony	770:775	arg1	factor					789:794	Granulocyte colony stimulating factor	758:794	Granulocyte colony stimulating factor (G-CSF)	758:802	CVS also inhibited secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum.
34699119	7	36	from	tract	1199:1203	arg1	content					1162:1168	the content	1158:1168	the content of acetic acid in intestinal tract of Il-10-/- mice	1158:1220	While CVS enhanced Regenerating Family Member 3 Gamma (Reg3γ), Mucin (Muc2, Muc3, and Muc4 gene expression, promoted intestinal epithelial cells to secrete Muc-2, and increased the content of acetic acid in intestinal tract of Il-10-/- mice.
34699119	6	37	theme	T	921:921	arg1	Cell					923:926	Normal T Cell	914:926	Normal T Cell Expressed and Presumably Secreted (RANTES) in serum	914:978	CVS also inhibited secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum.
34699119	6	37	theme	T	921:921	arg1	Activation					902:911	Activation	902:911	Activation	902:911	CVS also inhibited secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum.
34699119	0	38	theme	Spontaneous	35:45	arg1	Colitis					58:64	Spontaneous Ulcerative Colitis	35:64	Spontaneous Ulcerative Colitis in Il-10 Deficient Mice	35:88	Cereal Vinegar Sediment Alleviates Spontaneous Ulcerative Colitis in Il-10 Deficient Mice.
34699119	7	39	theme	Regenerating	1000:1011	arg1	Mucin					1044:1048	Mucin	1044:1048	Mucin (Muc2, Muc3, and Muc4 gene expression	1044:1086	While CVS enhanced Regenerating Family Member 3 Gamma (Reg3γ), Mucin (Muc2, Muc3, and Muc4 gene expression, promoted intestinal epithelial cells to secrete Muc-2, and increased the content of acetic acid in intestinal tract of Il-10-/- mice.
34699119	7	39	theme	Regenerating	1000:1011	arg1	Member					1020:1025	Regenerating Family Member 3 Gamma (Reg3γ)	1000:1041	Regenerating Family Member 3 Gamma (Reg3γ)	1000:1041	While CVS enhanced Regenerating Family Member 3 Gamma (Reg3γ), Mucin (Muc2, Muc3, and Muc4 gene expression, promoted intestinal epithelial cells to secrete Muc-2, and increased the content of acetic acid in intestinal tract of Il-10-/- mice.
34699119	7	40	theme	epithelial	1109:1118	arg1	cells					1120:1124	intestinal epithelial cells	1098:1124	intestinal epithelial cells	1098:1124	While CVS enhanced Regenerating Family Member 3 Gamma (Reg3γ), Mucin (Muc2, Muc3, and Muc4 gene expression, promoted intestinal epithelial cells to secrete Muc-2, and increased the content of acetic acid in intestinal tract of Il-10-/- mice.
34699119	8	41	theme	sensu	1407:1411	arg1	stricto					1413:1419	Clostridium sensu stricto 1	1395:1421	Clostridium sensu stricto 1	1395:1421	Additionally, CVS altered the composition of the gut microbiota by promoting the abundance of Akkermansia, Alistipes, and Lactobacillus, while inhibiting Desulfovibrio and Clostridium sensu stricto 1.
34699119	7	42	dep	Mucin	1044:1048	arg1	Muc3					1057:1060	Muc3	1057:1060	Muc3	1057:1060	While CVS enhanced Regenerating Family Member 3 Gamma (Reg3γ), Mucin (Muc2, Muc3, and Muc4 gene expression, promoted intestinal epithelial cells to secrete Muc-2, and increased the content of acetic acid in intestinal tract of Il-10-/- mice.
34699119	7	42	dep	Mucin	1044:1048	arg1	Muc4					1067:1070	Muc4	1067:1070	Muc4	1067:1070	While CVS enhanced Regenerating Family Member 3 Gamma (Reg3γ), Mucin (Muc2, Muc3, and Muc4 gene expression, promoted intestinal epithelial cells to secrete Muc-2, and increased the content of acetic acid in intestinal tract of Il-10-/- mice.
34699119	7	42	dep	Mucin	1044:1048	arg1	Muc2					1051:1054	Muc2	1051:1054	Muc2	1051:1054	While CVS enhanced Regenerating Family Member 3 Gamma (Reg3γ), Mucin (Muc2, Muc3, and Muc4 gene expression, promoted intestinal epithelial cells to secrete Muc-2, and increased the content of acetic acid in intestinal tract of Il-10-/- mice.
34699119	2	43	theme	various	197:203	arg1	anti-inflammatory					223:239	anti-inflammatory	223:239	anti-inflammatory	223:239	It has various functions such as anti-inflammatory, anti-tumor, hypoglycemic, and hypolipidemic.
34699119	2	43	theme	various	197:203	arg1	hypoglycemic					254:265	hypoglycemic	254:265	hypoglycemic	254:265	It has various functions such as anti-inflammatory, anti-tumor, hypoglycemic, and hypolipidemic.
34699119	2	43	theme	various	197:203	arg1	hypolipidemic					272:284	hypolipidemic	272:284	hypolipidemic	272:284	It has various functions such as anti-inflammatory, anti-tumor, hypoglycemic, and hypolipidemic.
34699119	2	43	theme	various	197:203	arg1	anti-tumor					242:251	anti-tumor	242:251	anti-tumor	242:251	It has various functions such as anti-inflammatory, anti-tumor, hypoglycemic, and hypolipidemic.
34699119	2	43	theme	various	197:203	arg1	functions					205:213	various functions	197:213	various functions such as anti-inflammatory, anti-tumor, hypoglycemic, and hypolipidemic	197:284	It has various functions such as anti-inflammatory, anti-tumor, hypoglycemic, and hypolipidemic.
34699119	10	44	theme	dietary	1690:1696	arg1	nutrient					1698:1705	a protective dietary nutrient	1677:1705	a protective dietary nutrient against colitis	1677:1721	CONCLUSION This study demonstrated that CVS ameliorates spontaneous ulcerative colitis in Il-10-/- mice, which suggests CVS supplementation may serve as a protective dietary nutrient against colitis.
34699119	10	44	theme	dietary	1690:1696	arg1	supplementation					1648:1662	CVS supplementation	1644:1662	CVS supplementation	1644:1662	CONCLUSION This study demonstrated that CVS ameliorates spontaneous ulcerative colitis in Il-10-/- mice, which suggests CVS supplementation may serve as a protective dietary nutrient against colitis.
34699119	6	45	theme	macrophage	817:826	arg1	GMCSF					855:859	GMCSF	855:859	GMCSF	855:859	CVS also inhibited secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum.
34699119	6	45	theme	macrophage	817:826	arg1	factor					847:852	granulocyte macrophage colony-stimulating factor	805:852	granulocyte macrophage colony-stimulating factor (GMCSF)	805:860	CVS also inhibited secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum.
34699119	9	46	theme	steroid	1474:1480	arg1	regulation					1460:1469	the regulation	1456:1469	the regulation of steroid, fatty acids, and bile acid biosynthesis	1456:1521	These changes may be related to the regulation of steroid, fatty acids, and bile acid biosynthesis.
34699119	4	47	theme	kg-1	397:400	arg1	weight					407:412	1 g kg-1 body weight	393:412	1 g kg-1 body weight	393:412	METHODS AND RESULTS CVS (1 g kg-1 body weight) is administered to mice for 42 days.
34699119	4	47	theme	kg-1	397:400	arg1	CVS					388:390	METHODS AND RESULTS CVS	368:390	METHODS AND RESULTS CVS (1 g kg-1 body weight)	368:413	METHODS AND RESULTS CVS (1 g kg-1 body weight) is administered to mice for 42 days.
34699119	5	48	theme	epithelium	467:476	arg1	damage					478:483	epithelium damage	467:483	epithelium damage	467:483	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	6	49	theme	factor	789:794	arg1	secretion					726:734	secretion	726:734	secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum	726:978	CVS also inhibited secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum.
34699119	9	50	theme	fatty	1483:1487	arg1	acids					1489:1493	fatty acids	1483:1493	fatty acids	1483:1493	These changes may be related to the regulation of steroid, fatty acids, and bile acid biosynthesis.
34699119	4	51	theme	METHODS	368:374	arg1	weight					407:412	1 g kg-1 body weight	393:412	1 g kg-1 body weight	393:412	METHODS AND RESULTS CVS (1 g kg-1 body weight) is administered to mice for 42 days.
34699119	4	51	theme	METHODS	368:374	arg1	CVS					388:390	METHODS AND RESULTS CVS	368:390	METHODS AND RESULTS CVS (1 g kg-1 body weight)	368:413	METHODS AND RESULTS CVS (1 g kg-1 body weight) is administered to mice for 42 days.
34699119	5	52	theme	necrosis	595:602	arg1	Interleukin					661:671	Interleukin	661:671	Interleukin(Il-23)	661:678	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	5	52	theme	necrosis	595:602	arg1	synthase					644:651	inducible nitric oxide synthase	621:651	inducible nitric oxide synthase (Inos)	621:658	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	5	52	theme	necrosis	595:602	arg1	-a					617:618	tumor necrosis factor (Tnf )-a	589:618	tumor necrosis factor (Tnf )-a	589:618	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	1	53	theme	vinegar	181:187	arg1	preservation					165:176	the preservation	161:176	the preservation of vinegar	161:187	SCOPE Cereal vinegar sediment (CVS) is precipitation generated during the preservation of vinegar.
34699119	4	54	theme	RESULTS	380:386	arg1	weight					407:412	1 g kg-1 body weight	393:412	1 g kg-1 body weight	393:412	METHODS AND RESULTS CVS (1 g kg-1 body weight) is administered to mice for 42 days.
34699119	4	54	theme	RESULTS	380:386	arg1	CVS					388:390	METHODS AND RESULTS CVS	368:390	METHODS AND RESULTS CVS (1 g kg-1 body weight)	368:413	METHODS AND RESULTS CVS (1 g kg-1 body weight) is administered to mice for 42 days.
34699119	10	55	theme	ulcerative	1592:1601	arg1	colitis					1603:1609	spontaneous ulcerative colitis	1580:1609	spontaneous ulcerative colitis	1580:1609	CONCLUSION This study demonstrated that CVS ameliorates spontaneous ulcerative colitis in Il-10-/- mice, which suggests CVS supplementation may serve as a protective dietary nutrient against colitis.
34699119	9	56	theme	bile	1500:1503	arg1	biosynthesis					1510:1521	bile acid biosynthesis	1500:1521	bile acid biosynthesis	1500:1521	These changes may be related to the regulation of steroid, fatty acids, and bile acid biosynthesis.
34699119	7	57	theme	mice	1217:1220	arg1	tract					1199:1203	intestinal tract	1188:1203	intestinal tract of Il-10-/- mice	1188:1220	While CVS enhanced Regenerating Family Member 3 Gamma (Reg3γ), Mucin (Muc2, Muc3, and Muc4 gene expression, promoted intestinal epithelial cells to secrete Muc-2, and increased the content of acetic acid in intestinal tract of Il-10-/- mice.
34699119	9	58	theme	biosynthesis	1510:1521	arg1	regulation					1460:1469	the regulation	1456:1469	the regulation of steroid, fatty acids, and bile acid biosynthesis	1456:1521	These changes may be related to the regulation of steroid, fatty acids, and bile acid biosynthesis.
34699119	8	59	theme	gut	1272:1274	arg1	microbiota					1276:1285	the gut microbiota	1268:1285	the gut microbiota	1268:1285	Additionally, CVS altered the composition of the gut microbiota by promoting the abundance of Akkermansia, Alistipes, and Lactobacillus, while inhibiting Desulfovibrio and Clostridium sensu stricto 1.
34699119	6	60	theme	factor	847:852	arg1	secretion					726:734	secretion	726:734	secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum	726:978	CVS also inhibited secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum.
34699119	7	61	theme	intestinal	1188:1197	arg1	tract					1199:1203	intestinal tract	1188:1203	intestinal tract of Il-10-/- mice	1188:1220	While CVS enhanced Regenerating Family Member 3 Gamma (Reg3γ), Mucin (Muc2, Muc3, and Muc4 gene expression, promoted intestinal epithelial cells to secrete Muc-2, and increased the content of acetic acid in intestinal tract of Il-10-/- mice.
34699119	5	62	dep	alleviated	456:465	arg1	inhibited					486:494	inhibited	486:494	inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level	486:557	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	5	63	theme	nitric	631:636	arg1	Inos					654:657	Inos	654:657	Inos	654:657	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	5	63	theme	nitric	631:636	arg1	synthase					644:651	inducible nitric oxide synthase	621:651	inducible nitric oxide synthase (Inos)	621:658	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	5	63	theme	nitric	631:636	arg1	-a					617:618	tumor necrosis factor (Tnf )-a	589:618	tumor necrosis factor (Tnf )-a	589:618	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	5	64	theme	malondialdehyde	531:545	arg1	level					553:557	malondialdehyde (MDA) level	531:557	malondialdehyde (MDA) level	531:557	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	7	65	theme	acid	1180:1183	arg1	content					1162:1168	the content	1158:1168	the content of acetic acid in intestinal tract of Il-10-/- mice	1158:1220	While CVS enhanced Regenerating Family Member 3 Gamma (Reg3γ), Mucin (Muc2, Muc3, and Muc4 gene expression, promoted intestinal epithelial cells to secrete Muc-2, and increased the content of acetic acid in intestinal tract of Il-10-/- mice.
34699119	0	66	theme	Deficient	75:83	arg1	Mice					85:88	Il-10 Deficient Mice	69:88	Il-10 Deficient Mice	69:88	Cereal Vinegar Sediment Alleviates Spontaneous Ulcerative Colitis in Il-10 Deficient Mice.
34699119	10	67	theme	CVS	1644:1646	arg1	nutrient					1698:1705	a protective dietary nutrient	1677:1705	a protective dietary nutrient against colitis	1677:1721	CONCLUSION This study demonstrated that CVS ameliorates spontaneous ulcerative colitis in Il-10-/- mice, which suggests CVS supplementation may serve as a protective dietary nutrient against colitis.
34699119	10	67	theme	CVS	1644:1646	arg1	supplementation					1648:1662	CVS supplementation	1644:1662	CVS supplementation	1644:1662	CONCLUSION This study demonstrated that CVS ameliorates spontaneous ulcerative colitis in Il-10-/- mice, which suggests CVS supplementation may serve as a protective dietary nutrient against colitis.
34699119	5	68	theme	myeloperoxidase	496:510	arg1	activity					518:525	myeloperoxidase (MPO) activity	496:525	myeloperoxidase (MPO) activity	496:525	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	6	69	theme	Regulated	887:895	arg1	secretion					726:734	secretion	726:734	secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum	726:978	CVS also inhibited secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum.
34699119	3	70	theme	spontaneous	330:340	arg1	colitis					342:348	spontaneous colitis	330:348	spontaneous colitis in Il-10-/- mice	330:365	This study evaluates the effects of CVS on spontaneous colitis in Il-10-/- mice.
34699119	5	71	theme	colon	683:687	arg1	tissues					689:695	colon tissues	683:695	colon tissues	683:695	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	3	72	theme	CVS	323:325	arg1	effects					312:318	the effects	308:318	the effects of CVS on spontaneous colitis in Il-10-/- mice	308:365	This study evaluates the effects of CVS on spontaneous colitis in Il-10-/- mice.
34699119	5	73	theme	decreased	560:568	arg1	expression					575:584	decreased gene expression	560:584	decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues	560:695	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	10	74	from	colitis	1603:1609	arg1	mice					1623:1626	Il-10-/- mice	1614:1626	Il-10-/- mice	1614:1626	CONCLUSION This study demonstrated that CVS ameliorates spontaneous ulcerative colitis in Il-10-/- mice, which suggests CVS supplementation may serve as a protective dietary nutrient against colitis.
34699119	8	75	theme	Lactobacillus	1345:1357	arg1	abundance					1304:1312	the abundance	1300:1312	the abundance of Akkermansia, Alistipes, and Lactobacillus	1300:1357	Additionally, CVS altered the composition of the gut microbiota by promoting the abundance of Akkermansia, Alistipes, and Lactobacillus, while inhibiting Desulfovibrio and Clostridium sensu stricto 1.
34699119	0	76	theme	Vinegar	7:13	arg1	Sediment					15:22	Cereal Vinegar Sediment	0:22	Cereal Vinegar Sediment	0:22	Cereal Vinegar Sediment Alleviates Spontaneous Ulcerative Colitis in Il-10 Deficient Mice.
34699119	5	77	theme	MDA	548:550	arg1	level					553:557	malondialdehyde (MDA) level	531:557	malondialdehyde (MDA) level	531:557	CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )-a, inducible nitric oxide synthase (Inos), Interleukin(Il-23) in colon tissues is found.
34699119	1	78	theme	SCOPE	91:95	arg1	CVS					122:124	CVS	122:124	CVS	122:124	SCOPE Cereal vinegar sediment (CVS) is precipitation generated during the preservation of vinegar.
34699119	1	78	theme	SCOPE	91:95	arg1	sediment					112:119	SCOPE Cereal vinegar sediment	91:119	SCOPE Cereal vinegar sediment (CVS)	91:125	SCOPE Cereal vinegar sediment (CVS) is precipitation generated during the preservation of vinegar.
34699119	7	79	from	acid	1180:1183	arg1	tract					1199:1203	intestinal tract	1188:1203	intestinal tract of Il-10-/- mice	1188:1220	While CVS enhanced Regenerating Family Member 3 Gamma (Reg3γ), Mucin (Muc2, Muc3, and Muc4 gene expression, promoted intestinal epithelial cells to secrete Muc-2, and increased the content of acetic acid in intestinal tract of Il-10-/- mice.
34699119	7	80	dep	Member	1020:1025	arg1	Gamma					1029:1033	Gamma	1029:1033	Regenerating Family Member 3 Gamma (Reg3γ)	1000:1041	While CVS enhanced Regenerating Family Member 3 Gamma (Reg3γ), Mucin (Muc2, Muc3, and Muc4 gene expression, promoted intestinal epithelial cells to secrete Muc-2, and increased the content of acetic acid in intestinal tract of Il-10-/- mice.
34699119	7	80	dep	Member	1020:1025	arg1	Reg3γ					1036:1040	Reg3γ	1036:1040	Reg3γ	1036:1040	While CVS enhanced Regenerating Family Member 3 Gamma (Reg3γ), Mucin (Muc2, Muc3, and Muc4 gene expression, promoted intestinal epithelial cells to secrete Muc-2, and increased the content of acetic acid in intestinal tract of Il-10-/- mice.
34699119	1	81	theme	vinegar	104:110	arg1	CVS					122:124	CVS	122:124	CVS	122:124	SCOPE Cereal vinegar sediment (CVS) is precipitation generated during the preservation of vinegar.
34699119	1	81	theme	vinegar	104:110	arg1	sediment					112:119	SCOPE Cereal vinegar sediment	91:119	SCOPE Cereal vinegar sediment (CVS)	91:125	SCOPE Cereal vinegar sediment (CVS) is precipitation generated during the preservation of vinegar.
34699119	10	82	theme	protective	1679:1688	arg1	nutrient					1698:1705	a protective dietary nutrient	1677:1705	a protective dietary nutrient against colitis	1677:1721	CONCLUSION This study demonstrated that CVS ameliorates spontaneous ulcerative colitis in Il-10-/- mice, which suggests CVS supplementation may serve as a protective dietary nutrient against colitis.
34699119	10	82	theme	protective	1679:1688	arg1	supplementation					1648:1662	CVS supplementation	1644:1662	CVS supplementation	1644:1662	CONCLUSION This study demonstrated that CVS ameliorates spontaneous ulcerative colitis in Il-10-/- mice, which suggests CVS supplementation may serve as a protective dietary nutrient against colitis.
34699119	7	83	theme	Family	1013:1018	arg1	Mucin					1044:1048	Mucin	1044:1048	Mucin (Muc2, Muc3, and Muc4 gene expression	1044:1086	While CVS enhanced Regenerating Family Member 3 Gamma (Reg3γ), Mucin (Muc2, Muc3, and Muc4 gene expression, promoted intestinal epithelial cells to secrete Muc-2, and increased the content of acetic acid in intestinal tract of Il-10-/- mice.
34699119	7	83	theme	Family	1013:1018	arg1	Member					1020:1025	Regenerating Family Member 3 Gamma (Reg3γ)	1000:1041	Regenerating Family Member 3 Gamma (Reg3γ)	1000:1041	While CVS enhanced Regenerating Family Member 3 Gamma (Reg3γ), Mucin (Muc2, Muc3, and Muc4 gene expression, promoted intestinal epithelial cells to secrete Muc-2, and increased the content of acetic acid in intestinal tract of Il-10-/- mice.
34699119	6	84	theme	Granulocyte	758:768	arg1	G-CSF					797:801	G-CSF	797:801	G-CSF	797:801	CVS also inhibited secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum.
34699119	6	84	theme	Granulocyte	758:768	arg1	factor					789:794	Granulocyte colony stimulating factor	758:794	Granulocyte colony stimulating factor (G-CSF)	758:802	CVS also inhibited secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum.
34699119	6	85	theme	Normal	914:919	arg1	Cell					923:926	Normal T Cell	914:926	Normal T Cell Expressed and Presumably Secreted (RANTES) in serum	914:978	CVS also inhibited secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum.
34699119	6	85	theme	Normal	914:919	arg1	Activation					902:911	Activation	902:911	Activation	902:911	CVS also inhibited secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum.
34699119	8	86	theme	Clostridium	1395:1405	arg1	stricto					1413:1419	Clostridium sensu stricto 1	1395:1421	Clostridium sensu stricto 1	1395:1421	Additionally, CVS altered the composition of the gut microbiota by promoting the abundance of Akkermansia, Alistipes, and Lactobacillus, while inhibiting Desulfovibrio and Clostridium sensu stricto 1.
34699119	6	87	theme	IL-13	751:755	arg1	secretion					726:734	secretion	726:734	secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum	726:978	CVS also inhibited secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum.
34699119	7	88	theme	intestinal	1098:1107	arg1	cells					1120:1124	intestinal epithelial cells	1098:1124	intestinal epithelial cells	1098:1124	While CVS enhanced Regenerating Family Member 3 Gamma (Reg3γ), Mucin (Muc2, Muc3, and Muc4 gene expression, promoted intestinal epithelial cells to secrete Muc-2, and increased the content of acetic acid in intestinal tract of Il-10-/- mice.
34699119	6	89	theme	IL-6	745:748	arg1	secretion					726:734	secretion	726:734	secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum	726:978	CVS also inhibited secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum.
34699119	6	90	theme	IL-2	739:742	arg1	secretion					726:734	secretion	726:734	secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum	726:978	CVS also inhibited secretion of IL-2, IL-6, IL-13, Granulocyte colony stimulating factor (G-CSF), granulocyte macrophage colony-stimulating factor (GMCSF), Interferon (IFN)-γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum.
34699119	7	91	theme	gene	1072:1075	arg1	expression					1077:1086	gene expression	1072:1086	gene expression	1072:1086	While CVS enhanced Regenerating Family Member 3 Gamma (Reg3γ), Mucin (Muc2, Muc3, and Muc4 gene expression, promoted intestinal epithelial cells to secrete Muc-2, and increased the content of acetic acid in intestinal tract of Il-10-/- mice.
34699119	3	92	from	effects	312:318	arg1	colitis					342:348	spontaneous colitis	330:348	spontaneous colitis in Il-10-/- mice	330:365	This study evaluates the effects of CVS on spontaneous colitis in Il-10-/- mice.
33588748	8	0	theme	inulin	1061:1066	arg1	fermentation					1068:1079	inulin fermentation	1061:1079	inulin fermentation	1061:1079	However, after inulin fermentation, the acetate concentration and inulin degradation rate decreased while the gas production increased in midlife group, suggesting a deficiency of saccharolytic potential in midlife, especially for non-digestible carbohydrate.
33588748	5	1	theme	age	779:781	arg1	groups					783:788	the two age groups	771:788	the two age groups	771:788	Although no difference was observed in the diversity indices between the two age groups, a wide range taxonomic changes were found in the faecal microbiota.
33588748	6	2	theme	fermented	944:952	arg1	samples					954:960	both faecal and fermented samples	928:960	both faecal and fermented samples	928:960	Furthermore, substantial Bifidobacterium reduction was also found in both faecal and fermented samples.
33588748	9	3	dep	CONCLUSIONS	1306:1316	arg1	demonstrate					1327:1337	demonstrate	1327:1337	demonstrate that gut microbiota begins to change as early as in midlife	1327:1397	CONCLUSIONS Our data demonstrate that gut microbiota begins to change as early as in midlife.
33588748	0	4	from	changes	12:18	arg1	midlife					37:43	midlife	37:43	midlife associated with reduced saccharolytic potential	37:91	Age-related changes of microbiota in midlife associated with reduced saccharolytic potential: an in vitro study.
33588748	4	5	theme	microbiota	635:644	arg1	responses					618:626	Metabolic responses	608:626	Metabolic responses of the microbiota	608:644	Metabolic responses of the microbiota were studied through in vitro batch fermentation model.
33588748	7	6	from	groups	1000:1005	arg1	similar					984:990	similar	984:990	similar	984:990	The faecal SCFAs are similar in both groups, as well as starch fermentation broth.
33588748	7	6	from	groups	1000:1005	arg1	SCFAs					974:978	The faecal SCFAs	963:978	The faecal SCFAs	963:978	The faecal SCFAs are similar in both groups, as well as starch fermentation broth.
33588748	0	7	theme	in	97:98	arg1	study					106:110	an in vitro study	94:110	Age-related changes of microbiota in midlife associated with reduced saccharolytic potential: an in vitro study.	0:111	Age-related changes of microbiota in midlife associated with reduced saccharolytic potential: an in vitro study.
33588748	10	8	from	elderly	1693:1699	arg1	period					1672:1677	this transition period	1656:1677	this transition period from young to elderly	1656:1699	The reduction in Bifidobacterium dominates the change of the microbiota composition in midlife resulting in attenuated saccharolytic capacity of inulin, possibly leading to insufficient acetate production which might be associated with healthy problems in this transition period from young to elderly.
33588748	10	8	from	elderly	1693:1699	arg1	problems					1644:1651	healthy problems	1636:1651	healthy problems in this transition period from young to elderly	1636:1699	The reduction in Bifidobacterium dominates the change of the microbiota composition in midlife resulting in attenuated saccharolytic capacity of inulin, possibly leading to insufficient acetate production which might be associated with healthy problems in this transition period from young to elderly.
33588748	10	9	theme	saccharolytic	1519:1531	arg1	capacity					1533:1540	attenuated saccharolytic capacity	1508:1540	attenuated saccharolytic capacity of inulin	1508:1550	The reduction in Bifidobacterium dominates the change of the microbiota composition in midlife resulting in attenuated saccharolytic capacity of inulin, possibly leading to insufficient acetate production which might be associated with healthy problems in this transition period from young to elderly.
33588748	9	10	from	midlife	1391:1397	arg1	early					1379:1383	early	1379:1383	early	1379:1383	CONCLUSIONS Our data demonstrate that gut microbiota begins to change as early as in midlife.
33588748	6	11	theme	faecal	933:938	arg1	samples					954:960	both faecal and fermented samples	928:960	both faecal and fermented samples	928:960	Furthermore, substantial Bifidobacterium reduction was also found in both faecal and fermented samples.
33588748	10	12	theme	inulin	1545:1550	arg1	capacity					1533:1540	attenuated saccharolytic capacity	1508:1540	attenuated saccharolytic capacity of inulin	1508:1550	The reduction in Bifidobacterium dominates the change of the microbiota composition in midlife resulting in attenuated saccharolytic capacity of inulin, possibly leading to insufficient acetate production which might be associated with healthy problems in this transition period from young to elderly.
33588748	5	13	located	found	827:831	arg2	changes					814:820	a wide range taxonomic changes	791:820	a wide range taxonomic changes	791:820	Although no difference was observed in the diversity indices between the two age groups, a wide range taxonomic changes were found in the faecal microbiota.
33588748	5	13	located	found	827:831	arg1	microbiota					847:856	the faecal microbiota	836:856	the faecal microbiota	836:856	Although no difference was observed in the diversity indices between the two age groups, a wide range taxonomic changes were found in the faecal microbiota.
33588748	10	14	dep	elderly	1693:1699	arg1	to					1690:1691	to	1690:1691	to	1690:1691	The reduction in Bifidobacterium dominates the change of the microbiota composition in midlife resulting in attenuated saccharolytic capacity of inulin, possibly leading to insufficient acetate production which might be associated with healthy problems in this transition period from young to elderly.
33588748	8	15	theme	non-digestible	1277:1290	arg1	carbohydrate					1292:1303	non-digestible carbohydrate	1277:1303	non-digestible carbohydrate	1277:1303	However, after inulin fermentation, the acetate concentration and inulin degradation rate decreased while the gas production increased in midlife group, suggesting a deficiency of saccharolytic potential in midlife, especially for non-digestible carbohydrate.
33588748	8	16	theme	potential	1240:1248	arg1	deficiency					1212:1221	a deficiency	1210:1221	a deficiency of saccharolytic potential in midlife	1210:1259	However, after inulin fermentation, the acetate concentration and inulin degradation rate decreased while the gas production increased in midlife group, suggesting a deficiency of saccharolytic potential in midlife, especially for non-digestible carbohydrate.
33588748	8	17	theme	saccharolytic	1226:1238	arg1	potential					1240:1248	saccharolytic potential	1226:1248	saccharolytic potential	1226:1248	However, after inulin fermentation, the acetate concentration and inulin degradation rate decreased while the gas production increased in midlife group, suggesting a deficiency of saccharolytic potential in midlife, especially for non-digestible carbohydrate.
33588748	7	18	theme	starch	1019:1024	arg1	broth					1039:1043	starch fermentation broth	1019:1043	starch fermentation broth	1019:1043	The faecal SCFAs are similar in both groups, as well as starch fermentation broth.
33588748	2	19	from	adulthood	317:325	arg1	general					330:336	general	330:336	general	330:336	Gut microbiota is reported to be stable across the long adulthood in general, but lack of careful examination, especially for the midlife people.
33588748	0	20	dep	in	97:98	arg1	vitro					100:104	vitro	100:104	vitro	100:104	Age-related changes of microbiota in midlife associated with reduced saccharolytic potential: an in vitro study.
33588748	5	21	theme	taxonomic	804:812	arg1	changes					814:820	a wide range taxonomic changes	791:820	a wide range taxonomic changes	791:820	Although no difference was observed in the diversity indices between the two age groups, a wide range taxonomic changes were found in the faecal microbiota.
33588748	1	22	theme	human	166:170	arg1	health					172:177	human health	166:177	human health	166:177	BACKGROUND Gut microbiota is critical in maintaining human health, of which diversity and abundance are subject to significantly reduce in seniors.
33588748	8	23	theme	gas	1156:1158	arg1	production					1160:1169	the gas production	1152:1169	the gas production	1152:1169	However, after inulin fermentation, the acetate concentration and inulin degradation rate decreased while the gas production increased in midlife group, suggesting a deficiency of saccharolytic potential in midlife, especially for non-digestible carbohydrate.
33588748	7	24	theme	fermentation	1026:1037	arg1	broth					1039:1043	starch fermentation broth	1019:1043	starch fermentation broth	1019:1043	The faecal SCFAs are similar in both groups, as well as starch fermentation broth.
33588748	2	25	theme	Gut	261:263	arg1	microbiota					265:274	Gut microbiota	261:274	Gut microbiota	261:274	Gut microbiota is reported to be stable across the long adulthood in general, but lack of careful examination, especially for the midlife people.
33588748	1	26	theme	health	172:177	arg1	subject					217:223	subject	217:223	subject	217:223	BACKGROUND Gut microbiota is critical in maintaining human health, of which diversity and abundance are subject to significantly reduce in seniors.
33588748	8	27	theme	degradation	1119:1129	arg1	rate					1131:1134	inulin degradation rate	1112:1134	inulin degradation rate	1112:1134	However, after inulin fermentation, the acetate concentration and inulin degradation rate decreased while the gas production increased in midlife group, suggesting a deficiency of saccharolytic potential in midlife, especially for non-digestible carbohydrate.
33588748	10	28	from	problems	1644:1651	arg1	period					1672:1677	this transition period	1656:1677	this transition period from young to elderly	1656:1699	The reduction in Bifidobacterium dominates the change of the microbiota composition in midlife resulting in attenuated saccharolytic capacity of inulin, possibly leading to insufficient acetate production which might be associated with healthy problems in this transition period from young to elderly.
33588748	10	28	from	problems	1644:1651	arg1	elderly					1693:1699	elderly	1693:1699	elderly	1693:1699	The reduction in Bifidobacterium dominates the change of the microbiota composition in midlife resulting in attenuated saccharolytic capacity of inulin, possibly leading to insufficient acetate production which might be associated with healthy problems in this transition period from young to elderly.
33588748	5	29	located	observed	729:736	arg2	difference					714:723	no difference	711:723	no difference	711:723	Although no difference was observed in the diversity indices between the two age groups, a wide range taxonomic changes were found in the faecal microbiota.
33588748	5	29	located	observed	729:736	arg1	indices					755:761	the diversity indices	741:761	the diversity indices between the two age groups	741:788	Although no difference was observed in the diversity indices between the two age groups, a wide range taxonomic changes were found in the faecal microbiota.
33588748	4	30	theme	fermentation	682:693	arg1	model					695:699	in vitro batch fermentation model	667:699	in vitro batch fermentation model	667:699	Metabolic responses of the microbiota were studied through in vitro batch fermentation model.
33588748	2	31	theme	careful	351:357	arg1	examination					359:369	careful examination	351:369	careful examination	351:369	Gut microbiota is reported to be stable across the long adulthood in general, but lack of careful examination, especially for the midlife people.
33588748	0	32	theme	Age-related	0:10	arg1	changes					12:18	Age-related changes	0:18	Age-related changes of microbiota in midlife associated with reduced saccharolytic potential: an in vitro study.	0:111	Age-related changes of microbiota in midlife associated with reduced saccharolytic potential: an in vitro study.
33588748	10	33	theme	acetate	1586:1592	arg1	production					1594:1603	insufficient acetate production	1573:1603	insufficient acetate production which might be associated with healthy problems in this transition period from young to elderly	1573:1699	The reduction in Bifidobacterium dominates the change of the microbiota composition in midlife resulting in attenuated saccharolytic capacity of inulin, possibly leading to insufficient acetate production which might be associated with healthy problems in this transition period from young to elderly.
33588748	4	34	theme	batch	676:680	arg1	model					695:699	in vitro batch fermentation model	667:699	in vitro batch fermentation model	667:699	Metabolic responses of the microbiota were studied through in vitro batch fermentation model.
33588748	4	35	theme	Metabolic	608:616	arg1	responses					618:626	Metabolic responses	608:626	Metabolic responses of the microbiota	608:644	Metabolic responses of the microbiota were studied through in vitro batch fermentation model.
33588748	0	36	theme	microbiota	23:32	arg1	changes					12:18	Age-related changes	0:18	Age-related changes of microbiota in midlife associated with reduced saccharolytic potential: an in vitro study.	0:111	Age-related changes of microbiota in midlife associated with reduced saccharolytic potential: an in vitro study.
33588748	10	37	theme	transition	1661:1670	arg1	period					1672:1677	this transition period	1656:1677	this transition period from young to elderly	1656:1699	The reduction in Bifidobacterium dominates the change of the microbiota composition in midlife resulting in attenuated saccharolytic capacity of inulin, possibly leading to insufficient acetate production which might be associated with healthy problems in this transition period from young to elderly.
33588748	9	38	from	early	1379:1383	arg1	midlife					1391:1397	midlife	1391:1397	midlife	1391:1397	CONCLUSIONS Our data demonstrate that gut microbiota begins to change as early as in midlife.
33588748	3	39	theme	healthy	522:528	arg1	people					530:535	healthy people	522:535	healthy people	522:535	RESULTS To characterize the gut microbiota in midlife, we investigated the faecal microbiota between two groups of healthy people, young, 20-39 years old, n = 15; and midlife, 40-60 years old, n = 15.
33588748	3	40	theme	gut	435:437	arg1	microbiota					439:448	the gut microbiota	431:448	the gut microbiota in midlife	431:459	RESULTS To characterize the gut microbiota in midlife, we investigated the faecal microbiota between two groups of healthy people, young, 20-39 years old, n = 15; and midlife, 40-60 years old, n = 15.
33588748	4	41	dep	in	667:668	arg1	vitro					670:674	vitro	670:674	vitro	670:674	Metabolic responses of the microbiota were studied through in vitro batch fermentation model.
33588748	3	42	theme	people	530:535	arg1	old					557:559	old	557:559	old	557:559	RESULTS To characterize the gut microbiota in midlife, we investigated the faecal microbiota between two groups of healthy people, young, 20-39 years old, n = 15; and midlife, 40-60 years old, n = 15.
33588748	3	42	theme	people	530:535	arg1	old					595:597	old	595:597	old	595:597	RESULTS To characterize the gut microbiota in midlife, we investigated the faecal microbiota between two groups of healthy people, young, 20-39 years old, n = 15; and midlife, 40-60 years old, n = 15.
33588748	3	42	theme	people	530:535	arg1	people					530:535	healthy people	522:535	healthy people	522:535	RESULTS To characterize the gut microbiota in midlife, we investigated the faecal microbiota between two groups of healthy people, young, 20-39 years old, n = 15; and midlife, 40-60 years old, n = 15.
33588748	3	42	theme	people	530:535	arg1	n = 15					562:567	n = 15	562:567	n = 15	562:567	RESULTS To characterize the gut microbiota in midlife, we investigated the faecal microbiota between two groups of healthy people, young, 20-39 years old, n = 15; and midlife, 40-60 years old, n = 15.
33588748	3	42	theme	people	530:535	arg1	young					538:542	young	538:542	young	538:542	RESULTS To characterize the gut microbiota in midlife, we investigated the faecal microbiota between two groups of healthy people, young, 20-39 years old, n = 15; and midlife, 40-60 years old, n = 15.
33588748	3	42	theme	people	530:535	arg1	n = 15					600:605	n = 15	600:605	n = 15	600:605	RESULTS To characterize the gut microbiota in midlife, we investigated the faecal microbiota between two groups of healthy people, young, 20-39 years old, n = 15; and midlife, 40-60 years old, n = 15.
33588748	3	42	theme	people	530:535	arg1	midlife					574:580	midlife	574:580	midlife	574:580	RESULTS To characterize the gut microbiota in midlife, we investigated the faecal microbiota between two groups of healthy people, young, 20-39 years old, n = 15; and midlife, 40-60 years old, n = 15.
33588748	3	42	theme	people	530:535	arg1	groups					512:517	two groups	508:517	two groups of healthy people	508:535	RESULTS To characterize the gut microbiota in midlife, we investigated the faecal microbiota between two groups of healthy people, young, 20-39 years old, n = 15; and midlife, 40-60 years old, n = 15.
33588748	5	43	theme	range	798:802	arg1	changes					814:820	a wide range taxonomic changes	791:820	a wide range taxonomic changes	791:820	Although no difference was observed in the diversity indices between the two age groups, a wide range taxonomic changes were found in the faecal microbiota.
33588748	8	44	theme	midlife	1184:1190	arg1	group					1192:1196	midlife group	1184:1196	midlife group	1184:1196	However, after inulin fermentation, the acetate concentration and inulin degradation rate decreased while the gas production increased in midlife group, suggesting a deficiency of saccharolytic potential in midlife, especially for non-digestible carbohydrate.
33588748	5	45	theme	wide	793:796	arg1	range					798:802	a wide range	791:802	a wide range taxonomic changes	791:820	Although no difference was observed in the diversity indices between the two age groups, a wide range taxonomic changes were found in the faecal microbiota.
33588748	10	46	theme	healthy	1636:1642	arg1	problems					1644:1651	healthy problems	1636:1651	healthy problems in this transition period from young to elderly	1636:1699	The reduction in Bifidobacterium dominates the change of the microbiota composition in midlife resulting in attenuated saccharolytic capacity of inulin, possibly leading to insufficient acetate production which might be associated with healthy problems in this transition period from young to elderly.
33588748	6	47	theme	Bifidobacterium	884:898	arg1	reduction					900:908	substantial Bifidobacterium reduction	872:908	substantial Bifidobacterium reduction	872:908	Furthermore, substantial Bifidobacterium reduction was also found in both faecal and fermented samples.
33588748	10	48	theme	attenuated	1508:1517	arg1	capacity					1533:1540	attenuated saccharolytic capacity	1508:1540	attenuated saccharolytic capacity of inulin	1508:1550	The reduction in Bifidobacterium dominates the change of the microbiota composition in midlife resulting in attenuated saccharolytic capacity of inulin, possibly leading to insufficient acetate production which might be associated with healthy problems in this transition period from young to elderly.
33588748	7	49	theme	faecal	967:972	arg1	similar					984:990	similar	984:990	similar	984:990	The faecal SCFAs are similar in both groups, as well as starch fermentation broth.
33588748	7	49	theme	faecal	967:972	arg1	SCFAs					974:978	The faecal SCFAs	963:978	The faecal SCFAs	963:978	The faecal SCFAs are similar in both groups, as well as starch fermentation broth.
33588748	5	50	theme	faecal	840:845	arg1	microbiota					847:856	the faecal microbiota	836:856	the faecal microbiota	836:856	Although no difference was observed in the diversity indices between the two age groups, a wide range taxonomic changes were found in the faecal microbiota.
33588748	5	51	theme	diversity	745:753	arg1	indices					755:761	the diversity indices	741:761	the diversity indices between the two age groups	741:788	Although no difference was observed in the diversity indices between the two age groups, a wide range taxonomic changes were found in the faecal microbiota.
33588748	6	52	theme	substantial	872:882	arg1	reduction					900:908	substantial Bifidobacterium reduction	872:908	substantial Bifidobacterium reduction	872:908	Furthermore, substantial Bifidobacterium reduction was also found in both faecal and fermented samples.
33588748	10	53	from	change	1447:1452	arg1	midlife					1487:1493	midlife	1487:1493	midlife resulting in attenuated saccharolytic capacity of inulin	1487:1550	The reduction in Bifidobacterium dominates the change of the microbiota composition in midlife resulting in attenuated saccharolytic capacity of inulin, possibly leading to insufficient acetate production which might be associated with healthy problems in this transition period from young to elderly.
33588748	1	54	theme	BACKGROUND	113:122	arg1	microbiota					128:137	BACKGROUND Gut microbiota	113:137	BACKGROUND Gut microbiota	113:137	BACKGROUND Gut microbiota is critical in maintaining human health, of which diversity and abundance are subject to significantly reduce in seniors.
33588748	10	55	theme	composition	1472:1482	arg1	change					1447:1452	the change	1443:1452	the change of the microbiota composition in midlife resulting in attenuated saccharolytic capacity of inulin	1443:1550	The reduction in Bifidobacterium dominates the change of the microbiota composition in midlife resulting in attenuated saccharolytic capacity of inulin, possibly leading to insufficient acetate production which might be associated with healthy problems in this transition period from young to elderly.
33588748	6	56	located	found	919:923	arg2	reduction					900:908	substantial Bifidobacterium reduction	872:908	substantial Bifidobacterium reduction	872:908	Furthermore, substantial Bifidobacterium reduction was also found in both faecal and fermented samples.
33588748	6	56	located	found	919:923	arg1	samples					954:960	both faecal and fermented samples	928:960	both faecal and fermented samples	928:960	Furthermore, substantial Bifidobacterium reduction was also found in both faecal and fermented samples.
33588748	3	57	from	microbiota	439:448	arg1	midlife					453:459	midlife	453:459	midlife	453:459	RESULTS To characterize the gut microbiota in midlife, we investigated the faecal microbiota between two groups of healthy people, young, 20-39 years old, n = 15; and midlife, 40-60 years old, n = 15.
33588748	1	58	theme	Gut	124:126	arg1	microbiota					128:137	BACKGROUND Gut microbiota	113:137	BACKGROUND Gut microbiota	113:137	BACKGROUND Gut microbiota is critical in maintaining human health, of which diversity and abundance are subject to significantly reduce in seniors.
33588748	8	59	theme	acetate	1086:1092	arg1	concentration					1094:1106	the acetate concentration	1082:1106	the acetate concentration	1082:1106	However, after inulin fermentation, the acetate concentration and inulin degradation rate decreased while the gas production increased in midlife group, suggesting a deficiency of saccharolytic potential in midlife, especially for non-digestible carbohydrate.
33588748	10	60	theme	microbiota	1461:1470	arg1	composition					1472:1482	the microbiota composition	1457:1482	the microbiota composition	1457:1482	The reduction in Bifidobacterium dominates the change of the microbiota composition in midlife resulting in attenuated saccharolytic capacity of inulin, possibly leading to insufficient acetate production which might be associated with healthy problems in this transition period from young to elderly.
33588748	8	61	theme	inulin	1112:1117	arg1	rate					1131:1134	inulin degradation rate	1112:1134	inulin degradation rate	1112:1134	However, after inulin fermentation, the acetate concentration and inulin degradation rate decreased while the gas production increased in midlife group, suggesting a deficiency of saccharolytic potential in midlife, especially for non-digestible carbohydrate.
33588748	8	62	from	deficiency	1212:1221	arg1	midlife					1253:1259	midlife	1253:1259	midlife	1253:1259	However, after inulin fermentation, the acetate concentration and inulin degradation rate decreased while the gas production increased in midlife group, suggesting a deficiency of saccharolytic potential in midlife, especially for non-digestible carbohydrate.
33588748	4	63	theme	in	667:668	arg1	model					695:699	in vitro batch fermentation model	667:699	in vitro batch fermentation model	667:699	Metabolic responses of the microbiota were studied through in vitro batch fermentation model.
33588748	2	64	theme	long	312:315	arg1	adulthood					317:325	the long adulthood	308:325	the long adulthood in general	308:336	Gut microbiota is reported to be stable across the long adulthood in general, but lack of careful examination, especially for the midlife people.
33588748	0	65	theme	saccharolytic	69:81	arg1	potential					83:91	reduced saccharolytic potential	61:91	reduced saccharolytic potential	61:91	Age-related changes of microbiota in midlife associated with reduced saccharolytic potential: an in vitro study.
33588748	0	66	dep	changes	12:18	arg1	study					106:110	an in vitro study	94:110	Age-related changes of microbiota in midlife associated with reduced saccharolytic potential: an in vitro study.	0:111	Age-related changes of microbiota in midlife associated with reduced saccharolytic potential: an in vitro study.
33588748	2	67	theme	midlife	391:397	arg1	people					399:404	the midlife people	387:404	the midlife people	387:404	Gut microbiota is reported to be stable across the long adulthood in general, but lack of careful examination, especially for the midlife people.
33588748	3	68	theme	faecal	482:487	arg1	microbiota					489:498	the faecal microbiota	478:498	the faecal microbiota between two groups of healthy people, young, 20-39 years old, n = 15; and midlife, 40-60 years old, n = 15	478:605	RESULTS To characterize the gut microbiota in midlife, we investigated the faecal microbiota between two groups of healthy people, young, 20-39 years old, n = 15; and midlife, 40-60 years old, n = 15.
33588748	2	69	theme	examination	359:369	arg1	lack					343:346	lack	343:346	lack of careful examination	343:369	Gut microbiota is reported to be stable across the long adulthood in general, but lack of careful examination, especially for the midlife people.
33588748	7	70	from	similar	984:990	arg1	groups					1000:1005	both groups	995:1005	both groups	995:1005	The faecal SCFAs are similar in both groups, as well as starch fermentation broth.
33588748	0	71	theme	reduced	61:67	arg1	potential					83:91	reduced saccharolytic potential	61:91	reduced saccharolytic potential	61:91	Age-related changes of microbiota in midlife associated with reduced saccharolytic potential: an in vitro study.
33588748	10	72	theme	insufficient	1573:1584	arg1	production					1594:1603	insufficient acetate production	1573:1603	insufficient acetate production which might be associated with healthy problems in this transition period from young to elderly	1573:1699	The reduction in Bifidobacterium dominates the change of the microbiota composition in midlife resulting in attenuated saccharolytic capacity of inulin, possibly leading to insufficient acetate production which might be associated with healthy problems in this transition period from young to elderly.
33588748	9	73	theme	gut	1344:1346	arg1	microbiota					1348:1357	gut microbiota	1344:1357	gut microbiota	1344:1357	CONCLUSIONS Our data demonstrate that gut microbiota begins to change as early as in midlife.
33588748	10	74	from	reduction	1404:1412	arg1	Bifidobacterium					1417:1431	Bifidobacterium	1417:1431	Bifidobacterium	1417:1431	The reduction in Bifidobacterium dominates the change of the microbiota composition in midlife resulting in attenuated saccharolytic capacity of inulin, possibly leading to insufficient acetate production which might be associated with healthy problems in this transition period from young to elderly.
34440934	8	0	from	O2	1136:1137	arg1	Chondrogenesis					1115:1128	Chondrogenesis	1115:1128	Chondrogenesis at 2% O2	1115:1137	Chondrogenesis at 2% O2 significantly reduced ALP gene expression and reduced type I collagen deposition, producing a more stable and less hypertrophic chondrogenic phenotype.
34440934	4	1	theme	different	546:554	arg1	media					564:568	two different culture media	542:568	two different culture media including specific growth factors, TGFβ1 or BMP2	542:617	We hypothesized that the use of two different culture media including specific growth factors, TGFβ1 or BMP2, as well as low (2% O2) or high (20% O2) oxygen tension, would improve the chondrogenic and osteogenic potential, respectively.
34440934	4	1	theme	different	546:554	arg1	factors					596:602	specific growth factors	580:602	specific growth factors	580:602	We hypothesized that the use of two different culture media including specific growth factors, TGFβ1 or BMP2, as well as low (2% O2) or high (20% O2) oxygen tension, would improve the chondrogenic and osteogenic potential, respectively.
34440934	7	2	theme	I	1066:1066	arg1	accumulation					1068:1079	collagen type II and I accumulation	1045:1079	collagen type II and I accumulation	1045:1079	Greater sGAG production and deposition, and collagen type II and I accumulation occurred for chondrogenic groups.
34440934	3	3	theme	conditions	473:482	arg1	effect					455:460	the effect	451:460	the effect of culture conditions on hBMSC differentiation	451:507	To improve our understanding of cartilage and bone in vitro differentiation, we investigated the effect of culture conditions on hBMSC differentiation.
34440934	4	4	theme	media	564:568	arg1	tension					667:673	low (2% O2) or high (20% O2) oxygen tension	631:673	low (2% O2) or high (20% O2) oxygen tension	631:673	We hypothesized that the use of two different culture media including specific growth factors, TGFβ1 or BMP2, as well as low (2% O2) or high (20% O2) oxygen tension, would improve the chondrogenic and osteogenic potential, respectively.
34440934	4	4	theme	media	564:568	arg1	use					535:537	the use	531:537	the use of two different culture media including specific growth factors, TGFβ1 or BMP2	531:617	We hypothesized that the use of two different culture media including specific growth factors, TGFβ1 or BMP2, as well as low (2% O2) or high (20% O2) oxygen tension, would improve the chondrogenic and osteogenic potential, respectively.
34440934	4	5	dep	high	646:649	arg1	%					654:654	20% O2	652:657	20% O2	652:657	We hypothesized that the use of two different culture media including specific growth factors, TGFβ1 or BMP2, as well as low (2% O2) or high (20% O2) oxygen tension, would improve the chondrogenic and osteogenic potential, respectively.
34440934	7	6	theme	collagen	1045:1052	arg1	type					1054:1057	collagen type II and I	1045:1066	type	1054:1057	Greater sGAG production and deposition, and collagen type II and I accumulation occurred for chondrogenic groups.
34440934	2	7	theme	culture	249:255	arg1	conditions					257:266	in vitro culture conditions	240:266	in vitro culture conditions for human bone marrow mesenchymal stromal cells (hBMSCs)	240:323	However, in vitro culture conditions for human bone marrow mesenchymal stromal cells (hBMSCs) have not yet been fully defined.
34440934	12	8	theme	same	1895:1898	arg1	population					1911:1920	the same donor cell population	1891:1920	the same donor cell population	1891:1920	This study demonstrates how the modulation of O2 tension, combined with tissue-specific growth factors and media composition can be tailored in vitro to promote chondral or endochondral differentiation while using the same donor cell population.
34440934	8	9	theme	I	1198:1198	arg1	collagen					1200:1207	type I collagen	1193:1207	type I collagen deposition	1193:1218	Chondrogenesis at 2% O2 significantly reduced ALP gene expression and reduced type I collagen deposition, producing a more stable and less hypertrophic chondrogenic phenotype.
34440934	3	10	from	effect	455:460	arg1	differentiation					493:507	hBMSC differentiation	487:507	hBMSC differentiation	487:507	To improve our understanding of cartilage and bone in vitro differentiation, we investigated the effect of culture conditions on hBMSC differentiation.
34440934	2	11	theme	mesenchymal	290:300	arg1	hBMSCs					317:322	hBMSCs	317:322	hBMSCs	317:322	However, in vitro culture conditions for human bone marrow mesenchymal stromal cells (hBMSCs) have not yet been fully defined.
34440934	2	11	theme	mesenchymal	290:300	arg1	cells					310:314	mesenchymal stromal cells	290:314	human bone marrow mesenchymal stromal cells (hBMSCs)	272:323	However, in vitro culture conditions for human bone marrow mesenchymal stromal cells (hBMSCs) have not yet been fully defined.
34440934	5	12	theme	hBMSCs	794:799	arg1	differentiation					775:789	Chondrogenic and osteogenic differentiation	747:789	Chondrogenic and osteogenic differentiation of hBMSCs isolated from multiple donors and expanded under the same conditions	747:868	Chondrogenic and osteogenic differentiation of hBMSCs isolated from multiple donors and expanded under the same conditions were directly compared.
34440934	12	13	theme	cell	1906:1909	arg1	population					1911:1920	the same donor cell population	1891:1920	the same donor cell population	1891:1920	This study demonstrates how the modulation of O2 tension, combined with tissue-specific growth factors and media composition can be tailored in vitro to promote chondral or endochondral differentiation while using the same donor cell population.
34440934	6	14	theme	Chondrogenic	894:905	arg1	groups					907:912	Chondrogenic groups	894:912	Chondrogenic groups	894:912	Chondrogenic groups showed a notable upregulation of chondrogenic markers compared with osteogenic groups.
34440934	2	15	theme	bone	278:281	arg1	marrow					283:288	human bone marrow mesenchymal stromal cells (hBMSCs)	272:323	human bone marrow mesenchymal stromal cells (hBMSCs)	272:323	However, in vitro culture conditions for human bone marrow mesenchymal stromal cells (hBMSCs) have not yet been fully defined.
34440934	1	16	theme	treatments	188:197	arg1	development					169:179	the development	165:179	the development of new treatments for cartilage and bone defects	165:228	In the field of tissue engineering, progress has been made towards the development of new treatments for cartilage and bone defects.
34440934	8	17	theme	ALP	1161:1163	arg1	expression					1170:1179	ALP gene expression	1161:1179	ALP gene expression	1161:1179	Chondrogenesis at 2% O2 significantly reduced ALP gene expression and reduced type I collagen deposition, producing a more stable and less hypertrophic chondrogenic phenotype.
34440934	7	18	theme	sGAG	1009:1012	arg1	production					1014:1023	Greater sGAG production	1001:1023	Greater sGAG production	1001:1023	Greater sGAG production and deposition, and collagen type II and I accumulation occurred for chondrogenic groups.
34440934	2	19	dep	marrow	283:288	arg1	hBMSCs					317:322	hBMSCs	317:322	hBMSCs	317:322	However, in vitro culture conditions for human bone marrow mesenchymal stromal cells (hBMSCs) have not yet been fully defined.
34440934	2	19	dep	marrow	283:288	arg1	cells					310:314	mesenchymal stromal cells	290:314	human bone marrow mesenchymal stromal cells (hBMSCs)	272:323	However, in vitro culture conditions for human bone marrow mesenchymal stromal cells (hBMSCs) have not yet been fully defined.
34440934	10	20	theme	osteogenic	1523:1532	arg1	media					1534:1538	BMP2 free osteogenic media	1513:1538	BMP2 free osteogenic media	1513:1538	An upregulation of ALP and OC occurred during osteogenesis in BMP2 containing media under 20% O2; BMP2 free osteogenic media downregulated ALP and also led to higher sGAG release.
34440934	1	21	theme	tissue	114:119	arg1	engineering					121:131	tissue engineering	114:131	tissue engineering	114:131	In the field of tissue engineering, progress has been made towards the development of new treatments for cartilage and bone defects.
34440934	4	22	theme	high	646:649	arg1	tension					667:673	low (2% O2) or high (20% O2) oxygen tension	631:673	low (2% O2) or high (20% O2) oxygen tension	631:673	We hypothesized that the use of two different culture media including specific growth factors, TGFβ1 or BMP2, as well as low (2% O2) or high (20% O2) oxygen tension, would improve the chondrogenic and osteogenic potential, respectively.
34440934	2	23	theme	in	240:241	arg1	conditions					257:266	in vitro culture conditions	240:266	in vitro culture conditions for human bone marrow mesenchymal stromal cells (hBMSCs)	240:323	However, in vitro culture conditions for human bone marrow mesenchymal stromal cells (hBMSCs) have not yet been fully defined.
34440934	6	24	theme	markers	960:966	arg1	upregulation					931:942	a notable upregulation	921:942	a notable upregulation of chondrogenic markers	921:966	Chondrogenic groups showed a notable upregulation of chondrogenic markers compared with osteogenic groups.
34440934	8	25	theme	hypertrophic	1254:1265	arg1	phenotype					1280:1288	a more stable and less hypertrophic chondrogenic phenotype	1231:1288	a more stable and less hypertrophic chondrogenic phenotype	1231:1288	Chondrogenesis at 2% O2 significantly reduced ALP gene expression and reduced type I collagen deposition, producing a more stable and less hypertrophic chondrogenic phenotype.
34440934	6	26	theme	notable	923:929	arg1	upregulation					931:942	a notable upregulation	921:942	a notable upregulation of chondrogenic markers	921:966	Chondrogenic groups showed a notable upregulation of chondrogenic markers compared with osteogenic groups.
34440934	4	27	theme	oxygen	660:665	arg1	tension					667:673	low (2% O2) or high (20% O2) oxygen tension	631:673	low (2% O2) or high (20% O2) oxygen tension	631:673	We hypothesized that the use of two different culture media including specific growth factors, TGFβ1 or BMP2, as well as low (2% O2) or high (20% O2) oxygen tension, would improve the chondrogenic and osteogenic potential, respectively.
34440934	12	28	theme	chondral	1838:1845	arg1	differentiation					1863:1877	chondral or endochondral differentiation	1838:1877	chondral or endochondral differentiation	1838:1877	This study demonstrates how the modulation of O2 tension, combined with tissue-specific growth factors and media composition can be tailored in vitro to promote chondral or endochondral differentiation while using the same donor cell population.
34440934	3	29	theme	bone	404:407	arg1	understanding					373:385	our understanding	369:385	our understanding of cartilage and bone in vitro differentiation	369:432	To improve our understanding of cartilage and bone in vitro differentiation, we investigated the effect of culture conditions on hBMSC differentiation.
34440934	10	30	contain	containing	1482:1491	arg1	BMP2					1477:1480	BMP2	1477:1480	BMP2 containing media under 20% O2	1477:1510	An upregulation of ALP and OC occurred during osteogenesis in BMP2 containing media under 20% O2; BMP2 free osteogenic media downregulated ALP and also led to higher sGAG release.
34440934	10	30	contain	containing	1482:1491	arg2	media					1493:1497	media	1493:1497	media	1493:1497	An upregulation of ALP and OC occurred during osteogenesis in BMP2 containing media under 20% O2; BMP2 free osteogenic media downregulated ALP and also led to higher sGAG release.
34440934	11	31	located	observed	1623:1630	arg2	mineralization					1604:1617	A higher mineralization	1595:1617	A higher mineralization	1595:1617	A higher mineralization was observed in the presence of BMP2 during osteogenesis.
34440934	11	31	located	observed	1623:1630	arg1	presence					1639:1646	the presence	1635:1646	the presence of BMP2	1635:1654	A higher mineralization was observed in the presence of BMP2 during osteogenesis.
34440934	3	32	theme	cartilage	390:398	arg1	understanding					373:385	our understanding	369:385	our understanding of cartilage and bone in vitro differentiation	369:432	To improve our understanding of cartilage and bone in vitro differentiation, we investigated the effect of culture conditions on hBMSC differentiation.
34440934	0	33	theme	Mesenchymal	0:10	arg1	Differentiation					25:39	Mesenchymal Stromal Cell Differentiation	0:39	Mesenchymal Stromal Cell Differentiation for Generating Cartilage and Bone-Like Tissues In Vitro.	0:96	Mesenchymal Stromal Cell Differentiation for Generating Cartilage and Bone-Like Tissues In Vitro.
34440934	10	34	theme	ALP	1434:1436	arg1	upregulation					1418:1429	An upregulation	1415:1429	An upregulation of ALP and OC	1415:1443	An upregulation of ALP and OC occurred during osteogenesis in BMP2 containing media under 20% O2; BMP2 free osteogenic media downregulated ALP and also led to higher sGAG release.
34440934	0	35	theme	Cell	20:23	arg1	Differentiation					25:39	Mesenchymal Stromal Cell Differentiation	0:39	Mesenchymal Stromal Cell Differentiation for Generating Cartilage and Bone-Like Tissues In Vitro.	0:96	Mesenchymal Stromal Cell Differentiation for Generating Cartilage and Bone-Like Tissues In Vitro.
34440934	4	36	theme	osteogenic	711:720	arg1	potential					722:730	the chondrogenic and osteogenic potential	690:730	the chondrogenic and osteogenic potential	690:730	We hypothesized that the use of two different culture media including specific growth factors, TGFβ1 or BMP2, as well as low (2% O2) or high (20% O2) oxygen tension, would improve the chondrogenic and osteogenic potential, respectively.
34440934	10	37	theme	sGAG	1581:1584	arg1	release					1586:1592	higher sGAG release	1574:1592	higher sGAG release	1574:1592	An upregulation of ALP and OC occurred during osteogenesis in BMP2 containing media under 20% O2; BMP2 free osteogenic media downregulated ALP and also led to higher sGAG release.
34440934	7	38	theme	chondrogenic	1094:1105	arg1	groups					1107:1112	chondrogenic groups	1094:1112	chondrogenic groups	1094:1112	Greater sGAG production and deposition, and collagen type II and I accumulation occurred for chondrogenic groups.
34440934	0	39	dep	Cartilage	56:64	arg1	Tissues					80:86	Tissues	80:86	Tissues	80:86	Mesenchymal Stromal Cell Differentiation for Generating Cartilage and Bone-Like Tissues In Vitro.
34440934	12	40	theme	tension	1726:1732	arg1	modulation					1709:1718	the modulation	1705:1718	the modulation of O2 tension, combined with tissue-specific growth factors and media composition	1705:1800	This study demonstrates how the modulation of O2 tension, combined with tissue-specific growth factors and media composition can be tailored in vitro to promote chondral or endochondral differentiation while using the same donor cell population.
34440934	11	41	theme	higher	1597:1602	arg1	mineralization					1604:1617	A higher mineralization	1595:1617	A higher mineralization	1595:1617	A higher mineralization was observed in the presence of BMP2 during osteogenesis.
34440934	9	42	theme	%	1309:1309	arg1	tension					1297:1303	An O2 tension	1291:1303	An O2 tension of 2%	1291:1309	An O2 tension of 2% did not inhibit osteogenic differentiation at the protein level but reduced ALP and OC gene expression.
34440934	3	43	dep	in	409:410	arg1	vitro					412:416	vitro	412:416	vitro	412:416	To improve our understanding of cartilage and bone in vitro differentiation, we investigated the effect of culture conditions on hBMSC differentiation.
34440934	10	44	theme	OC	1442:1443	arg1	upregulation					1418:1429	An upregulation	1415:1429	An upregulation of ALP and OC	1415:1443	An upregulation of ALP and OC occurred during osteogenesis in BMP2 containing media under 20% O2; BMP2 free osteogenic media downregulated ALP and also led to higher sGAG release.
34440934	12	45	theme	growth	1765:1770	arg1	factors					1772:1778	tissue-specific growth factors	1749:1778	tissue-specific growth factors	1749:1778	This study demonstrates how the modulation of O2 tension, combined with tissue-specific growth factors and media composition can be tailored in vitro to promote chondral or endochondral differentiation while using the same donor cell population.
34440934	4	46	theme	chondrogenic	694:705	arg1	potential					722:730	the chondrogenic and osteogenic potential	690:730	the chondrogenic and osteogenic potential	690:730	We hypothesized that the use of two different culture media including specific growth factors, TGFβ1 or BMP2, as well as low (2% O2) or high (20% O2) oxygen tension, would improve the chondrogenic and osteogenic potential, respectively.
34440934	9	47	theme	osteogenic	1327:1336	arg1	differentiation					1338:1352	osteogenic differentiation	1327:1352	osteogenic differentiation	1327:1352	An O2 tension of 2% did not inhibit osteogenic differentiation at the protein level but reduced ALP and OC gene expression.
34440934	4	48	theme	growth	589:594	arg1	BMP2					614:617	BMP2	614:617	BMP2	614:617	We hypothesized that the use of two different culture media including specific growth factors, TGFβ1 or BMP2, as well as low (2% O2) or high (20% O2) oxygen tension, would improve the chondrogenic and osteogenic potential, respectively.
34440934	4	48	theme	growth	589:594	arg1	TGFβ1					605:609	TGFβ1	605:609	TGFβ1	605:609	We hypothesized that the use of two different culture media including specific growth factors, TGFβ1 or BMP2, as well as low (2% O2) or high (20% O2) oxygen tension, would improve the chondrogenic and osteogenic potential, respectively.
34440934	4	48	theme	growth	589:594	arg1	factors					596:602	specific growth factors	580:602	specific growth factors	580:602	We hypothesized that the use of two different culture media including specific growth factors, TGFβ1 or BMP2, as well as low (2% O2) or high (20% O2) oxygen tension, would improve the chondrogenic and osteogenic potential, respectively.
34440934	3	49	theme	culture	465:471	arg1	conditions					473:482	culture conditions	465:482	culture conditions	465:482	To improve our understanding of cartilage and bone in vitro differentiation, we investigated the effect of culture conditions on hBMSC differentiation.
34440934	7	50	theme	type	1054:1057	arg1	accumulation					1068:1079	collagen type II and I accumulation	1045:1079	collagen type II and I accumulation	1045:1079	Greater sGAG production and deposition, and collagen type II and I accumulation occurred for chondrogenic groups.
34440934	2	51	theme	human	272:276	arg1	marrow					283:288	human bone marrow mesenchymal stromal cells (hBMSCs)	272:323	human bone marrow mesenchymal stromal cells (hBMSCs)	272:323	However, in vitro culture conditions for human bone marrow mesenchymal stromal cells (hBMSCs) have not yet been fully defined.
34440934	9	52	theme	protein	1361:1367	arg1	level					1369:1373	the protein level	1357:1373	the protein level	1357:1373	An O2 tension of 2% did not inhibit osteogenic differentiation at the protein level but reduced ALP and OC gene expression.
34440934	4	53	theme	culture	556:562	arg1	media					564:568	two different culture media	542:568	two different culture media including specific growth factors, TGFβ1 or BMP2	542:617	We hypothesized that the use of two different culture media including specific growth factors, TGFβ1 or BMP2, as well as low (2% O2) or high (20% O2) oxygen tension, would improve the chondrogenic and osteogenic potential, respectively.
34440934	4	53	theme	culture	556:562	arg1	factors					596:602	specific growth factors	580:602	specific growth factors	580:602	We hypothesized that the use of two different culture media including specific growth factors, TGFβ1 or BMP2, as well as low (2% O2) or high (20% O2) oxygen tension, would improve the chondrogenic and osteogenic potential, respectively.
34440934	5	54	theme	osteogenic	764:773	arg1	differentiation					775:789	Chondrogenic and osteogenic differentiation	747:789	Chondrogenic and osteogenic differentiation of hBMSCs isolated from multiple donors and expanded under the same conditions	747:868	Chondrogenic and osteogenic differentiation of hBMSCs isolated from multiple donors and expanded under the same conditions were directly compared.
34440934	10	55	theme	free	1518:1521	arg1	media					1534:1538	BMP2 free osteogenic media	1513:1538	BMP2 free osteogenic media	1513:1538	An upregulation of ALP and OC occurred during osteogenesis in BMP2 containing media under 20% O2; BMP2 free osteogenic media downregulated ALP and also led to higher sGAG release.
34440934	2	56	theme	stromal	302:308	arg1	hBMSCs					317:322	hBMSCs	317:322	hBMSCs	317:322	However, in vitro culture conditions for human bone marrow mesenchymal stromal cells (hBMSCs) have not yet been fully defined.
34440934	2	56	theme	stromal	302:308	arg1	cells					310:314	mesenchymal stromal cells	290:314	human bone marrow mesenchymal stromal cells (hBMSCs)	272:323	However, in vitro culture conditions for human bone marrow mesenchymal stromal cells (hBMSCs) have not yet been fully defined.
34440934	9	57	theme	ALP	1387:1389	arg1	expression					1403:1412	ALP and OC gene expression	1387:1412	ALP and OC gene expression	1387:1412	An O2 tension of 2% did not inhibit osteogenic differentiation at the protein level but reduced ALP and OC gene expression.
34440934	8	58	theme	2	1133:1133	arg1	%					1134:1134	%	1134:1134	%	1134:1134	Chondrogenesis at 2% O2 significantly reduced ALP gene expression and reduced type I collagen deposition, producing a more stable and less hypertrophic chondrogenic phenotype.
34440934	8	59	theme	type	1193:1196	arg1	collagen					1200:1207	type I collagen	1193:1207	type I collagen deposition	1193:1218	Chondrogenesis at 2% O2 significantly reduced ALP gene expression and reduced type I collagen deposition, producing a more stable and less hypertrophic chondrogenic phenotype.
34440934	1	60	theme	new	184:186	arg1	treatments					188:197	new treatments	184:197	new treatments for cartilage and bone defects	184:228	In the field of tissue engineering, progress has been made towards the development of new treatments for cartilage and bone defects.
34440934	12	61	theme	donor	1900:1904	arg1	population					1911:1920	the same donor cell population	1891:1920	the same donor cell population	1891:1920	This study demonstrates how the modulation of O2 tension, combined with tissue-specific growth factors and media composition can be tailored in vitro to promote chondral or endochondral differentiation while using the same donor cell population.
34440934	8	62	theme	collagen	1200:1207	arg1	deposition					1209:1218	type I collagen deposition	1193:1218	type I collagen deposition	1193:1218	Chondrogenesis at 2% O2 significantly reduced ALP gene expression and reduced type I collagen deposition, producing a more stable and less hypertrophic chondrogenic phenotype.
34440934	7	63	theme	Greater	1001:1007	arg1	production					1014:1023	Greater sGAG production	1001:1023	Greater sGAG production	1001:1023	Greater sGAG production and deposition, and collagen type II and I accumulation occurred for chondrogenic groups.
34440934	2	64	dep	in	240:241	arg1	vitro					243:247	vitro	243:247	vitro	243:247	However, in vitro culture conditions for human bone marrow mesenchymal stromal cells (hBMSCs) have not yet been fully defined.
34440934	5	65	theme	Chondrogenic	747:758	arg1	differentiation					775:789	Chondrogenic and osteogenic differentiation	747:789	Chondrogenic and osteogenic differentiation of hBMSCs isolated from multiple donors and expanded under the same conditions	747:868	Chondrogenic and osteogenic differentiation of hBMSCs isolated from multiple donors and expanded under the same conditions were directly compared.
34440934	12	66	theme	endochondral	1850:1861	arg1	differentiation					1863:1877	chondral or endochondral differentiation	1838:1877	chondral or endochondral differentiation	1838:1877	This study demonstrates how the modulation of O2 tension, combined with tissue-specific growth factors and media composition can be tailored in vitro to promote chondral or endochondral differentiation while using the same donor cell population.
34440934	8	67	theme	gene	1165:1168	arg1	expression					1170:1179	ALP gene expression	1161:1179	ALP gene expression	1161:1179	Chondrogenesis at 2% O2 significantly reduced ALP gene expression and reduced type I collagen deposition, producing a more stable and less hypertrophic chondrogenic phenotype.
34440934	1	68	theme	engineering	121:131	arg1	field					105:109	the field	101:109	the field of tissue engineering	101:131	In the field of tissue engineering, progress has been made towards the development of new treatments for cartilage and bone defects.
34440934	8	69	theme	stable	1238:1243	arg1	phenotype					1280:1288	a more stable and less hypertrophic chondrogenic phenotype	1231:1288	a more stable and less hypertrophic chondrogenic phenotype	1231:1288	Chondrogenesis at 2% O2 significantly reduced ALP gene expression and reduced type I collagen deposition, producing a more stable and less hypertrophic chondrogenic phenotype.
34440934	9	70	theme	OC	1395:1396	arg1	expression					1403:1412	ALP and OC gene expression	1387:1412	ALP and OC gene expression	1387:1412	An O2 tension of 2% did not inhibit osteogenic differentiation at the protein level but reduced ALP and OC gene expression.
34440934	6	71	theme	chondrogenic	947:958	arg1	markers					960:966	chondrogenic markers	947:966	chondrogenic markers	947:966	Chondrogenic groups showed a notable upregulation of chondrogenic markers compared with osteogenic groups.
34440934	4	72	theme	low	631:633	arg1	tension					667:673	low (2% O2) or high (20% O2) oxygen tension	631:673	low (2% O2) or high (20% O2) oxygen tension	631:673	We hypothesized that the use of two different culture media including specific growth factors, TGFβ1 or BMP2, as well as low (2% O2) or high (20% O2) oxygen tension, would improve the chondrogenic and osteogenic potential, respectively.
34440934	11	73	theme	BMP2	1651:1654	arg1	presence					1639:1646	the presence	1635:1646	the presence of BMP2	1635:1654	A higher mineralization was observed in the presence of BMP2 during osteogenesis.
34440934	5	74	theme	multiple	815:822	arg1	donors					824:829	multiple donors	815:829	multiple donors	815:829	Chondrogenic and osteogenic differentiation of hBMSCs isolated from multiple donors and expanded under the same conditions were directly compared.
34440934	8	75	theme	chondrogenic	1267:1278	arg1	phenotype					1280:1288	a more stable and less hypertrophic chondrogenic phenotype	1231:1288	a more stable and less hypertrophic chondrogenic phenotype	1231:1288	Chondrogenesis at 2% O2 significantly reduced ALP gene expression and reduced type I collagen deposition, producing a more stable and less hypertrophic chondrogenic phenotype.
34440934	9	76	theme	gene	1398:1401	arg1	expression					1403:1412	ALP and OC gene expression	1387:1412	ALP and OC gene expression	1387:1412	An O2 tension of 2% did not inhibit osteogenic differentiation at the protein level but reduced ALP and OC gene expression.
34440934	3	77	theme	in	409:410	arg1	differentiation					418:432	in vitro differentiation	409:432	in vitro differentiation	409:432	To improve our understanding of cartilage and bone in vitro differentiation, we investigated the effect of culture conditions on hBMSC differentiation.
34440934	0	78	theme	Stromal	12:18	arg1	Differentiation					25:39	Mesenchymal Stromal Cell Differentiation	0:39	Mesenchymal Stromal Cell Differentiation for Generating Cartilage and Bone-Like Tissues In Vitro.	0:96	Mesenchymal Stromal Cell Differentiation for Generating Cartilage and Bone-Like Tissues In Vitro.
34440934	5	79	theme	same	854:857	arg1	conditions					859:868	the same conditions	850:868	the same conditions	850:868	Chondrogenic and osteogenic differentiation of hBMSCs isolated from multiple donors and expanded under the same conditions were directly compared.
34440934	12	80	theme	media	1784:1788	arg1	modulation					1709:1718	the modulation	1705:1718	the modulation of O2 tension, combined with tissue-specific growth factors and media composition	1705:1800	This study demonstrates how the modulation of O2 tension, combined with tissue-specific growth factors and media composition can be tailored in vitro to promote chondral or endochondral differentiation while using the same donor cell population.
34440934	12	81	dep	tension	1726:1732	arg1	composition					1790:1800	composition	1790:1800	composition	1790:1800	This study demonstrates how the modulation of O2 tension, combined with tissue-specific growth factors and media composition can be tailored in vitro to promote chondral or endochondral differentiation while using the same donor cell population.
34440934	3	82	dep	cartilage	390:398	arg1	differentiation					418:432	in vitro differentiation	409:432	in vitro differentiation	409:432	To improve our understanding of cartilage and bone in vitro differentiation, we investigated the effect of culture conditions on hBMSC differentiation.
34440934	6	83	theme	osteogenic	982:991	arg1	groups					993:998	osteogenic groups	982:998	osteogenic groups	982:998	Chondrogenic groups showed a notable upregulation of chondrogenic markers compared with osteogenic groups.
34440934	5	84	dep	hBMSCs	794:799	arg1	expanded					835:842	expanded	835:842	expanded under the same conditions	835:868	Chondrogenic and osteogenic differentiation of hBMSCs isolated from multiple donors and expanded under the same conditions were directly compared.
34440934	5	84	dep	hBMSCs	794:799	arg1	isolated					801:808	isolated	801:808	isolated from multiple donors	801:829	Chondrogenic and osteogenic differentiation of hBMSCs isolated from multiple donors and expanded under the same conditions were directly compared.
34440934	10	85	theme	higher	1574:1579	arg1	release					1586:1592	higher sGAG release	1574:1592	higher sGAG release	1574:1592	An upregulation of ALP and OC occurred during osteogenesis in BMP2 containing media under 20% O2; BMP2 free osteogenic media downregulated ALP and also led to higher sGAG release.
34440934	12	86	theme	tissue-specific	1749:1763	arg1	factors					1772:1778	tissue-specific growth factors	1749:1778	tissue-specific growth factors	1749:1778	This study demonstrates how the modulation of O2 tension, combined with tissue-specific growth factors and media composition can be tailored in vitro to promote chondral or endochondral differentiation while using the same donor cell population.
34440934	4	87	theme	specific	580:587	arg1	BMP2					614:617	BMP2	614:617	BMP2	614:617	We hypothesized that the use of two different culture media including specific growth factors, TGFβ1 or BMP2, as well as low (2% O2) or high (20% O2) oxygen tension, would improve the chondrogenic and osteogenic potential, respectively.
34440934	4	87	theme	specific	580:587	arg1	TGFβ1					605:609	TGFβ1	605:609	TGFβ1	605:609	We hypothesized that the use of two different culture media including specific growth factors, TGFβ1 or BMP2, as well as low (2% O2) or high (20% O2) oxygen tension, would improve the chondrogenic and osteogenic potential, respectively.
34440934	4	87	theme	specific	580:587	arg1	factors					596:602	specific growth factors	580:602	specific growth factors	580:602	We hypothesized that the use of two different culture media including specific growth factors, TGFβ1 or BMP2, as well as low (2% O2) or high (20% O2) oxygen tension, would improve the chondrogenic and osteogenic potential, respectively.
34440934	4	88	dep	low	631:633	arg1	%					637:637	2% O2	636:640	2% O2	636:640	We hypothesized that the use of two different culture media including specific growth factors, TGFβ1 or BMP2, as well as low (2% O2) or high (20% O2) oxygen tension, would improve the chondrogenic and osteogenic potential, respectively.
34440934	1	89	dep	cartilage	203:211	arg1	defects					222:228	defects	222:228	defects	222:228	In the field of tissue engineering, progress has been made towards the development of new treatments for cartilage and bone defects.
34440934	3	90	theme	hBMSC	487:491	arg1	differentiation					493:507	hBMSC differentiation	487:507	hBMSC differentiation	487:507	To improve our understanding of cartilage and bone in vitro differentiation, we investigated the effect of culture conditions on hBMSC differentiation.
32284410	8	0	theme	common	1153:1158	arg1	signal					1160:1165	a common signal	1151:1165	a common signal with specific machinery that initiates discrete signaling cascades to produce a localized response	1151:1264	Our results demonstrate that distinct membrane domains can integrate a common signal with specific machinery that initiates discrete signaling cascades to produce a localized response.
32284410	1	1	from	differences	184:194	arg1	protein					199:205	protein	199:205	protein	199:205	The plasma membrane (PM) is composed of heterogeneous subdomains, characterized by differences in protein and lipid composition.
32284410	1	1	from	differences	184:194	arg1	composition					217:227	lipid composition	211:227	lipid composition	211:227	The plasma membrane (PM) is composed of heterogeneous subdomains, characterized by differences in protein and lipid composition.
32284410	0	2	from	specificity	78:88	arg1	plants					93:98	plants	93:98	plants	93:98	Chitin perception in plasmodesmata characterizes submembrane immune-signaling specificity in plants.
32284410	5	3	dep	kinases	718:724	arg1	kinases					718:724	the LysM receptor kinases	700:724	the LysM receptor kinases LYK4 and LYK5 in addition to LYM2	700:758	Focusing on chitin signaling, we found that responses in the plasmodesmal PM require the LysM receptor kinases LYK4 and LYK5 in addition to LYM2.
32284410	5	3	dep	kinases	718:724	arg1	LYK5					735:738	LYK5	735:738	LYK5	735:738	Focusing on chitin signaling, we found that responses in the plasmodesmal PM require the LysM receptor kinases LYK4 and LYK5 in addition to LYM2.
32284410	5	3	dep	kinases	718:724	arg1	LYK4					726:729	LYK4	726:729	LYK4	726:729	Focusing on chitin signaling, we found that responses in the plasmodesmal PM require the LysM receptor kinases LYK4 and LYK5 in addition to LYM2.
32284410	0	4	from	perception	7:16	arg1	plasmodesmata					21:33	plasmodesmata	21:33	plasmodesmata	21:33	Chitin perception in plasmodesmata characterizes submembrane immune-signaling specificity in plants.
32284410	7	5	theme	chitin-induced	944:957	arg1	production					959:968	chitin-induced production	944:968	chitin-induced production of reactive oxygen species and callose	944:1007	We further uncovered that chitin-induced production of reactive oxygen species and callose depends on specific signaling events that lead to plasmodesmata closure.
32284410	3	6	theme	plasmodesmal	366:377	arg1	microdomain					396:406	a discrete microdomain	385:406	a discrete microdomain that hosts specific receptors and responses	385:450	In plants, the plasmodesmal PM is a discrete microdomain that hosts specific receptors and responses.
32284410	3	6	theme	plasmodesmal	366:377	arg1	PM					379:380	the plasmodesmal PM	362:380	the plasmodesmal PM	362:380	In plants, the plasmodesmal PM is a discrete microdomain that hosts specific receptors and responses.
32284410	4	7	theme	membrane	520:527	arg1	domains					529:535	membrane domains	520:535	membrane domains	520:535	We exploited the independence of this PM domain to investigate how membrane domains can independently integrate a signal that triggers responses across the cell.
32284410	7	8	theme	reactive	973:980	arg1	species					989:995	reactive oxygen species	973:995	reactive oxygen species	973:995	We further uncovered that chitin-induced production of reactive oxygen species and callose depends on specific signaling events that lead to plasmodesmata closure.
32284410	2	9	theme	extracellular	328:340	arg1	stimuli					342:348	extracellular stimuli	328:348	extracellular stimuli	328:348	PM receptors can be dynamically sorted into membrane domains to underpin signaling in response to extracellular stimuli.
32284410	4	10	theme	PM	491:492	arg1	domain					494:499	this PM domain	486:499	this PM domain	486:499	We exploited the independence of this PM domain to investigate how membrane domains can independently integrate a signal that triggers responses across the cell.
32284410	5	11	theme	plasmodesmal	676:687	arg1	PM					689:690	the plasmodesmal PM	672:690	the plasmodesmal PM	672:690	Focusing on chitin signaling, we found that responses in the plasmodesmal PM require the LysM receptor kinases LYK4 and LYK5 in addition to LYM2.
32284410	5	12	theme	chitin	627:632	arg1	signaling					634:642	chitin signaling	627:642	chitin signaling	627:642	Focusing on chitin signaling, we found that responses in the plasmodesmal PM require the LysM receptor kinases LYK4 and LYK5 in addition to LYM2.
32284410	8	13	theme	specific	1172:1179	arg1	machinery					1181:1189	specific machinery	1172:1189	specific machinery that initiates discrete signaling cascades to produce a localized response	1172:1264	Our results demonstrate that distinct membrane domains can integrate a common signal with specific machinery that initiates discrete signaling cascades to produce a localized response.
32284410	0	14	theme	submembrane	49:59	arg1	specificity					78:88	submembrane immune-signaling specificity	49:88	submembrane immune-signaling specificity in plants	49:98	Chitin perception in plasmodesmata characterizes submembrane immune-signaling specificity in plants.
32284410	8	15	theme	localized	1247:1255	arg1	response					1257:1264	a localized response	1245:1264	a localized response	1245:1264	Our results demonstrate that distinct membrane domains can integrate a common signal with specific machinery that initiates discrete signaling cascades to produce a localized response.
32284410	3	16	theme	discrete	387:394	arg1	microdomain					396:406	a discrete microdomain	385:406	a discrete microdomain that hosts specific receptors and responses	385:450	In plants, the plasmodesmal PM is a discrete microdomain that hosts specific receptors and responses.
32284410	3	16	theme	discrete	387:394	arg1	PM					379:380	the plasmodesmal PM	362:380	the plasmodesmal PM	362:380	In plants, the plasmodesmal PM is a discrete microdomain that hosts specific receptors and responses.
32284410	4	17	theme	domain	494:499	arg1	independence					470:481	the independence	466:481	the independence of this PM domain	466:499	We exploited the independence of this PM domain to investigate how membrane domains can independently integrate a signal that triggers responses across the cell.
32284410	1	18	theme	plasma	105:110	arg1	membrane					112:119	The plasma membrane	101:119	The plasma membrane (PM)	101:124	The plasma membrane (PM) is composed of heterogeneous subdomains, characterized by differences in protein and lipid composition.
32284410	1	18	theme	plasma	105:110	arg1	PM					122:123	PM	122:123	PM	122:123	The plasma membrane (PM) is composed of heterogeneous subdomains, characterized by differences in protein and lipid composition.
32284410	0	19	theme	Chitin	0:5	arg1	perception					7:16	Chitin perception	0:16	Chitin perception in plasmodesmata	0:33	Chitin perception in plasmodesmata characterizes submembrane immune-signaling specificity in plants.
32284410	8	20	theme	signaling	1215:1223	arg1	cascades					1225:1232	discrete signaling cascades	1206:1232	discrete signaling cascades	1206:1232	Our results demonstrate that distinct membrane domains can integrate a common signal with specific machinery that initiates discrete signaling cascades to produce a localized response.
32284410	5	21	theme	receptor	709:716	arg1	kinases					718:724	the LysM receptor kinases	700:724	the LysM receptor kinases LYK4 and LYK5 in addition to LYM2	700:758	Focusing on chitin signaling, we found that responses in the plasmodesmal PM require the LysM receptor kinases LYK4 and LYK5 in addition to LYM2.
32284410	5	21	theme	receptor	709:716	arg1	LYK5					735:738	LYK5	735:738	LYK5	735:738	Focusing on chitin signaling, we found that responses in the plasmodesmal PM require the LysM receptor kinases LYK4 and LYK5 in addition to LYM2.
32284410	5	21	theme	receptor	709:716	arg1	LYK4					726:729	LYK4	726:729	LYK4	726:729	Focusing on chitin signaling, we found that responses in the plasmodesmal PM require the LysM receptor kinases LYK4 and LYK5 in addition to LYM2.
32284410	6	22	theme	dynamic	776:782	arg1	changes					784:790	dynamic changes	776:790	dynamic changes in the localization, association, or mobility of these receptors	776:855	Chitin induces dynamic changes in the localization, association, or mobility of these receptors, but only LYM2 and LYK4 are detected in the plasmodesmal PM.
32284410	6	23	dep	localization	799:810	arg1	the					795:797	the	795:797	the	795:797	Chitin induces dynamic changes in the localization, association, or mobility of these receptors, but only LYM2 and LYK4 are detected in the plasmodesmal PM.
32284410	6	24	from	changes	784:790	arg1	association					813:823	association	813:823	association	813:823	Chitin induces dynamic changes in the localization, association, or mobility of these receptors, but only LYM2 and LYK4 are detected in the plasmodesmal PM.
32284410	6	24	from	changes	784:790	arg1	mobility					829:836	mobility	829:836	mobility	829:836	Chitin induces dynamic changes in the localization, association, or mobility of these receptors, but only LYM2 and LYK4 are detected in the plasmodesmal PM.
32284410	6	24	from	changes	784:790	arg1	localization					799:810	localization	799:810	localization	799:810	Chitin induces dynamic changes in the localization, association, or mobility of these receptors, but only LYM2 and LYK4 are detected in the plasmodesmal PM.
32284410	8	25	theme	discrete	1206:1213	arg1	cascades					1225:1232	discrete signaling cascades	1206:1232	discrete signaling cascades	1206:1232	Our results demonstrate that distinct membrane domains can integrate a common signal with specific machinery that initiates discrete signaling cascades to produce a localized response.
32284410	7	26	theme	signaling	1029:1037	arg1	events					1039:1044	specific signaling events	1020:1044	specific signaling events that lead to plasmodesmata closure	1020:1079	We further uncovered that chitin-induced production of reactive oxygen species and callose depends on specific signaling events that lead to plasmodesmata closure.
32284410	1	27	theme	lipid	211:215	arg1	composition					217:227	lipid composition	211:227	lipid composition	211:227	The plasma membrane (PM) is composed of heterogeneous subdomains, characterized by differences in protein and lipid composition.
32284410	3	28	theme	specific	419:426	arg1	receptors					428:436	specific receptors	419:436	specific receptors	419:436	In plants, the plasmodesmal PM is a discrete microdomain that hosts specific receptors and responses.
32284410	6	29	located	detected	885:892	arg2	LYM2					867:870	LYM2	867:870	LYM2	867:870	Chitin induces dynamic changes in the localization, association, or mobility of these receptors, but only LYM2 and LYK4 are detected in the plasmodesmal PM.
32284410	6	29	located	detected	885:892	arg1	PM					914:915	the plasmodesmal PM	897:915	the plasmodesmal PM	897:915	Chitin induces dynamic changes in the localization, association, or mobility of these receptors, but only LYM2 and LYK4 are detected in the plasmodesmal PM.
32284410	6	29	located	detected	885:892	arg2	LYK4					876:879	LYK4	876:879	LYK4	876:879	Chitin induces dynamic changes in the localization, association, or mobility of these receptors, but only LYM2 and LYK4 are detected in the plasmodesmal PM.
32284410	8	30	theme	distinct	1111:1118	arg1	domains					1129:1135	distinct membrane domains	1111:1135	distinct membrane domains	1111:1135	Our results demonstrate that distinct membrane domains can integrate a common signal with specific machinery that initiates discrete signaling cascades to produce a localized response.
32284410	6	31	theme	receptors	847:855	arg1	association					813:823	association	813:823	association	813:823	Chitin induces dynamic changes in the localization, association, or mobility of these receptors, but only LYM2 and LYK4 are detected in the plasmodesmal PM.
32284410	6	31	theme	receptors	847:855	arg1	mobility					829:836	mobility	829:836	mobility	829:836	Chitin induces dynamic changes in the localization, association, or mobility of these receptors, but only LYM2 and LYK4 are detected in the plasmodesmal PM.
32284410	6	31	theme	receptors	847:855	arg1	localization					799:810	localization	799:810	localization	799:810	Chitin induces dynamic changes in the localization, association, or mobility of these receptors, but only LYM2 and LYK4 are detected in the plasmodesmal PM.
32284410	0	32	theme	immune-signaling	61:76	arg1	specificity					78:88	submembrane immune-signaling specificity	49:88	submembrane immune-signaling specificity in plants	49:98	Chitin perception in plasmodesmata characterizes submembrane immune-signaling specificity in plants.
32284410	5	33	from	responses	659:667	arg1	PM					689:690	the plasmodesmal PM	672:690	the plasmodesmal PM	672:690	Focusing on chitin signaling, we found that responses in the plasmodesmal PM require the LysM receptor kinases LYK4 and LYK5 in addition to LYM2.
32284410	7	34	theme	plasmodesmata	1059:1071	arg1	closure					1073:1079	plasmodesmata closure	1059:1079	plasmodesmata closure	1059:1079	We further uncovered that chitin-induced production of reactive oxygen species and callose depends on specific signaling events that lead to plasmodesmata closure.
32284410	2	35	theme	PM	230:231	arg1	receptors					233:241	PM receptors	230:241	PM receptors	230:241	PM receptors can be dynamically sorted into membrane domains to underpin signaling in response to extracellular stimuli.
32284410	8	36	with	signal	1160:1165	arg1	machinery					1181:1189	specific machinery	1172:1189	specific machinery that initiates discrete signaling cascades to produce a localized response	1172:1264	Our results demonstrate that distinct membrane domains can integrate a common signal with specific machinery that initiates discrete signaling cascades to produce a localized response.
32284410	7	37	theme	specific	1020:1027	arg1	events					1039:1044	specific signaling events	1020:1044	specific signaling events that lead to plasmodesmata closure	1020:1079	We further uncovered that chitin-induced production of reactive oxygen species and callose depends on specific signaling events that lead to plasmodesmata closure.
32284410	2	38	theme	membrane	274:281	arg1	domains					283:289	membrane domains	274:289	membrane domains	274:289	PM receptors can be dynamically sorted into membrane domains to underpin signaling in response to extracellular stimuli.
32284410	7	39	theme	callose	1001:1007	arg1	production					959:968	chitin-induced production	944:968	chitin-induced production of reactive oxygen species and callose	944:1007	We further uncovered that chitin-induced production of reactive oxygen species and callose depends on specific signaling events that lead to plasmodesmata closure.
32284410	5	40	theme	LysM	704:707	arg1	kinases					718:724	the LysM receptor kinases	700:724	the LysM receptor kinases LYK4 and LYK5 in addition to LYM2	700:758	Focusing on chitin signaling, we found that responses in the plasmodesmal PM require the LysM receptor kinases LYK4 and LYK5 in addition to LYM2.
32284410	5	40	theme	LysM	704:707	arg1	LYK5					735:738	LYK5	735:738	LYK5	735:738	Focusing on chitin signaling, we found that responses in the plasmodesmal PM require the LysM receptor kinases LYK4 and LYK5 in addition to LYM2.
32284410	5	40	theme	LysM	704:707	arg1	LYK4					726:729	LYK4	726:729	LYK4	726:729	Focusing on chitin signaling, we found that responses in the plasmodesmal PM require the LysM receptor kinases LYK4 and LYK5 in addition to LYM2.
32284410	3	41	from	microdomain	396:406	arg1	plants					354:359	plants	354:359	plants	354:359	In plants, the plasmodesmal PM is a discrete microdomain that hosts specific receptors and responses.
32284410	7	42	theme	oxygen	982:987	arg1	species					989:995	reactive oxygen species	973:995	reactive oxygen species	973:995	We further uncovered that chitin-induced production of reactive oxygen species and callose depends on specific signaling events that lead to plasmodesmata closure.
32284410	2	43	from	signaling	303:311	arg1	response					316:323	response	316:323	response to extracellular stimuli	316:348	PM receptors can be dynamically sorted into membrane domains to underpin signaling in response to extracellular stimuli.
32284410	7	44	theme	species	989:995	arg1	production					959:968	chitin-induced production	944:968	chitin-induced production of reactive oxygen species and callose	944:1007	We further uncovered that chitin-induced production of reactive oxygen species and callose depends on specific signaling events that lead to plasmodesmata closure.
32284410	1	45	theme	heterogeneous	141:153	arg1	subdomains					155:164	heterogeneous subdomains	141:164	heterogeneous subdomains	141:164	The plasma membrane (PM) is composed of heterogeneous subdomains, characterized by differences in protein and lipid composition.
32284410	6	46	theme	plasmodesmal	901:912	arg1	PM					914:915	the plasmodesmal PM	897:915	the plasmodesmal PM	897:915	Chitin induces dynamic changes in the localization, association, or mobility of these receptors, but only LYM2 and LYK4 are detected in the plasmodesmal PM.
32284410	8	47	theme	membrane	1120:1127	arg1	domains					1129:1135	distinct membrane domains	1111:1135	distinct membrane domains	1111:1135	Our results demonstrate that distinct membrane domains can integrate a common signal with specific machinery that initiates discrete signaling cascades to produce a localized response.
33483018	0	0	theme	mulching	94:101	arg1	film					103:106	sand-fixation liquid mulching film	73:106	sand-fixation liquid mulching film	73:106	Preparation and characterization of an eco-friendly dust suppression and sand-fixation liquid mulching film.
33483018	7	1	from	environments	1290:1301	arg1	demand					1210:1215	the demand	1206:1215	the demand of dust suppression and sand-fixation in dusty areas and desertification environments, which opens a new application field for liquid mulching film with high safety and environmental protection	1206:1409	The CLS-HWPU film meets the demand of dust suppression and sand-fixation in dusty areas and desertification environments, which opens a new application field for liquid mulching film with high safety and environmental protection.
33483018	5	2	theme	electron	899:906	arg1	terms					810:814	terms	810:814	terms of light transmittance	810:837	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	5	2	theme	electron	899:906	arg1	SEM					920:922	SEM	920:922	SEM	920:922	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	5	2	theme	electron	899:906	arg1	microscopy					908:917	scanning electron microscopy	890:917	scanning electron microscopy (SEM)	890:923	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	7	3	theme	suppression	1225:1235	arg1	demand					1210:1215	the demand	1206:1215	the demand of dust suppression and sand-fixation in dusty areas and desertification environments, which opens a new application field for liquid mulching film with high safety and environmental protection	1206:1409	The CLS-HWPU film meets the demand of dust suppression and sand-fixation in dusty areas and desertification environments, which opens a new application field for liquid mulching film with high safety and environmental protection.
33483018	0	4	theme	liquid	87:92	arg1	film					103:106	sand-fixation liquid mulching film	73:106	sand-fixation liquid mulching film	73:106	Preparation and characterization of an eco-friendly dust suppression and sand-fixation liquid mulching film.
33483018	7	5	theme	sand-fixation	1241:1253	arg1	demand					1210:1215	the demand	1206:1215	the demand of dust suppression and sand-fixation in dusty areas and desertification environments, which opens a new application field for liquid mulching film with high safety and environmental protection	1206:1409	The CLS-HWPU film meets the demand of dust suppression and sand-fixation in dusty areas and desertification environments, which opens a new application field for liquid mulching film with high safety and environmental protection.
33483018	5	6	theme	degradation	840:850	arg1	terms					810:814	terms	810:814	terms of light transmittance	810:837	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	5	6	theme	degradation	840:850	arg1	test					864:867	degradation performance test	840:867	degradation performance test	840:867	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	5	7	theme	erosion	1035:1041	arg1	terms					810:814	terms	810:814	terms of light transmittance	810:837	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	5	7	theme	erosion	1035:1041	arg1	test					1054:1057	erosion resistance test	1035:1057	erosion resistance test	1035:1057	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	7	8	theme	liquid	1344:1349	arg1	film					1360:1363	liquid mulching film	1344:1363	liquid mulching film with high safety and environmental protection	1344:1409	The CLS-HWPU film meets the demand of dust suppression and sand-fixation in dusty areas and desertification environments, which opens a new application field for liquid mulching film with high safety and environmental protection.
33483018	3	9	from	properties	560:569	arg1	states					586:591	dry and wet states	574:591	dry and wet states	574:591	The effects of liquid mulching film composition on mechanical properties in dry and wet states were investigated.
33483018	5	10	dep	spectrometer	971:982	arg1	ATR-FTIR					985:992	ATR-FTIR	985:992	ATR-FTIR	985:992	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	7	11	from	demand	1210:1215	arg1	environments					1290:1301	desertification environments	1274:1301	desertification environments	1274:1301	The CLS-HWPU film meets the demand of dust suppression and sand-fixation in dusty areas and desertification environments, which opens a new application field for liquid mulching film with high safety and environmental protection.
33483018	7	11	from	demand	1210:1215	arg1	areas					1264:1268	dusty areas	1258:1268	dusty areas	1258:1268	The CLS-HWPU film meets the demand of dust suppression and sand-fixation in dusty areas and desertification environments, which opens a new application field for liquid mulching film with high safety and environmental protection.
33483018	2	12	theme	dissolved	290:298	arg1	acid					306:309	dissolved humic acid	290:309	dissolved humic acid (HA)	290:314	Water polyurethane (WPU) was blended with dissolved humic acid (HA) firstly, and then the blend solutions (HWPU) were sprayed on the surface of cationic starch (CS) / sodium lignosulfonate (LS) film to synthesize the liquid mulching film (CLS-HWPU).
33483018	2	12	theme	dissolved	290:298	arg1	HA					312:313	HA	312:313	HA	312:313	Water polyurethane (WPU) was blended with dissolved humic acid (HA) firstly, and then the blend solutions (HWPU) were sprayed on the surface of cationic starch (CS) / sodium lignosulfonate (LS) film to synthesize the liquid mulching film (CLS-HWPU).
33483018	2	13	theme	humic	300:304	arg1	acid					306:309	dissolved humic acid	290:309	dissolved humic acid (HA)	290:314	Water polyurethane (WPU) was blended with dissolved humic acid (HA) firstly, and then the blend solutions (HWPU) were sprayed on the surface of cationic starch (CS) / sodium lignosulfonate (LS) film to synthesize the liquid mulching film (CLS-HWPU).
33483018	2	13	theme	humic	300:304	arg1	HA					312:313	HA	312:313	HA	312:313	Water polyurethane (WPU) was blended with dissolved humic acid (HA) firstly, and then the blend solutions (HWPU) were sprayed on the surface of cationic starch (CS) / sodium lignosulfonate (LS) film to synthesize the liquid mulching film (CLS-HWPU).
33483018	5	14	theme	thermo	996:1001	arg1	terms					810:814	terms	810:814	terms of light transmittance	810:837	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	5	14	theme	thermo	996:1001	arg1	TGA					1025:1027	TGA	1025:1027	TGA	1025:1027	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	5	14	theme	thermo	996:1001	arg1	analysis					1015:1022	thermo gravimetric analysis	996:1022	thermo gravimetric analysis (TGA)	996:1028	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	0	15	theme	film	103:106	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of an eco-friendly dust suppression and sand-fixation liquid mulching film.
33483018	0	15	theme	film	103:106	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of an eco-friendly dust suppression and sand-fixation liquid mulching film.
33483018	5	16	dep	reflectance-Fourier	932:950	arg1	transform					952:960	transform	952:960	transform infrared spectrometer (ATR-FTIR)	952:993	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	7	17	from	suppression	1225:1235	arg1	environments					1290:1301	desertification environments	1274:1301	desertification environments	1274:1301	The CLS-HWPU film meets the demand of dust suppression and sand-fixation in dusty areas and desertification environments, which opens a new application field for liquid mulching film with high safety and environmental protection.
33483018	7	17	from	suppression	1225:1235	arg1	areas					1264:1268	dusty areas	1258:1268	dusty areas	1258:1268	The CLS-HWPU film meets the demand of dust suppression and sand-fixation in dusty areas and desertification environments, which opens a new application field for liquid mulching film with high safety and environmental protection.
33483018	7	18	from	areas	1264:1268	arg1	demand					1210:1215	the demand	1206:1215	the demand of dust suppression and sand-fixation in dusty areas and desertification environments, which opens a new application field for liquid mulching film with high safety and environmental protection	1206:1409	The CLS-HWPU film meets the demand of dust suppression and sand-fixation in dusty areas and desertification environments, which opens a new application field for liquid mulching film with high safety and environmental protection.
33483018	4	19	theme	optimal	640:646	arg1	%					690:690	3%	689:690	3% (CS)	689:695	The results showed that the optimal composition of liquid mulching film was: 3% (CS), 0.9 % (LS), 1.5 % (glycerol), 2% (HA), and 30 % (WPU).
33483018	4	19	theme	optimal	640:646	arg1	composition					648:658	the optimal composition	636:658	the optimal composition of liquid mulching film	636:682	The results showed that the optimal composition of liquid mulching film was: 3% (CS), 0.9 % (LS), 1.5 % (glycerol), 2% (HA), and 30 % (WPU).
33483018	5	20	theme	gravimetric	1003:1013	arg1	terms					810:814	terms	810:814	terms of light transmittance	810:837	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	5	20	theme	gravimetric	1003:1013	arg1	TGA					1025:1027	TGA	1025:1027	TGA	1025:1027	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	5	20	theme	gravimetric	1003:1013	arg1	analysis					1015:1022	thermo gravimetric analysis	996:1022	thermo gravimetric analysis (TGA)	996:1028	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	5	21	theme	CLS-HWPU	757:764	arg1	films					782:786	The CLS-HWPU liquid mulching films	753:786	The CLS-HWPU liquid mulching films	753:786	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	2	22	theme	film	442:445	arg1	surface					381:387	the surface	377:387	the surface of cationic starch (CS) / sodium lignosulfonate (LS) film to synthesize the liquid mulching film (CLS-HWPU)	377:495	Water polyurethane (WPU) was blended with dissolved humic acid (HA) firstly, and then the blend solutions (HWPU) were sprayed on the surface of cationic starch (CS) / sodium lignosulfonate (LS) film to synthesize the liquid mulching film (CLS-HWPU).
33483018	5	23	theme	scanning	890:897	arg1	terms					810:814	terms	810:814	terms of light transmittance	810:837	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	5	23	theme	scanning	890:897	arg1	SEM					920:922	SEM	920:922	SEM	920:922	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	5	23	theme	scanning	890:897	arg1	microscopy					908:917	scanning electron microscopy	890:917	scanning electron microscopy (SEM)	890:923	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	2	24	theme	sodium	415:420	arg1	film					442:445	cationic starch (CS) / sodium lignosulfonate (LS) film	392:445	cationic starch (CS) / sodium lignosulfonate (LS) film	392:445	Water polyurethane (WPU) was blended with dissolved humic acid (HA) firstly, and then the blend solutions (HWPU) were sprayed on the surface of cationic starch (CS) / sodium lignosulfonate (LS) film to synthesize the liquid mulching film (CLS-HWPU).
33483018	5	25	theme	liquid	766:771	arg1	films					782:786	The CLS-HWPU liquid mulching films	753:786	The CLS-HWPU liquid mulching films	753:786	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	7	26	theme	dust	1220:1223	arg1	suppression					1225:1235	dust suppression	1220:1235	dust suppression	1220:1235	The CLS-HWPU film meets the demand of dust suppression and sand-fixation in dusty areas and desertification environments, which opens a new application field for liquid mulching film with high safety and environmental protection.
33483018	7	27	theme	application	1322:1332	arg1	field					1334:1338	a new application field	1316:1338	a new application field for liquid mulching film with high safety and environmental protection	1316:1409	The CLS-HWPU film meets the demand of dust suppression and sand-fixation in dusty areas and desertification environments, which opens a new application field for liquid mulching film with high safety and environmental protection.
33483018	2	28	theme	lignosulfonate	422:435	arg1	film					442:445	cationic starch (CS) / sodium lignosulfonate (LS) film	392:445	cationic starch (CS) / sodium lignosulfonate (LS) film	392:445	Water polyurethane (WPU) was blended with dissolved humic acid (HA) firstly, and then the blend solutions (HWPU) were sprayed on the surface of cationic starch (CS) / sodium lignosulfonate (LS) film to synthesize the liquid mulching film (CLS-HWPU).
33483018	4	29	theme	liquid	663:668	arg1	film					679:682	liquid mulching film	663:682	liquid mulching film	663:682	The results showed that the optimal composition of liquid mulching film was: 3% (CS), 0.9 % (LS), 1.5 % (glycerol), 2% (HA), and 30 % (WPU).
33483018	2	30	theme	starch	401:406	arg1	film					442:445	cationic starch (CS) / sodium lignosulfonate (LS) film	392:445	cationic starch (CS) / sodium lignosulfonate (LS) film	392:445	Water polyurethane (WPU) was blended with dissolved humic acid (HA) firstly, and then the blend solutions (HWPU) were sprayed on the surface of cationic starch (CS) / sodium lignosulfonate (LS) film to synthesize the liquid mulching film (CLS-HWPU).
33483018	5	31	theme	infrared	962:969	arg1	spectrometer					971:982	infrared spectrometer	962:982	infrared spectrometer (ATR-FTIR)	962:993	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	7	32	theme	desertification	1274:1288	arg1	environments					1290:1301	desertification environments	1274:1301	desertification environments	1274:1301	The CLS-HWPU film meets the demand of dust suppression and sand-fixation in dusty areas and desertification environments, which opens a new application field for liquid mulching film with high safety and environmental protection.
33483018	7	33	theme	CLS-HWPU	1186:1193	arg1	film					1195:1198	The CLS-HWPU film	1182:1198	The CLS-HWPU film	1182:1198	The CLS-HWPU film meets the demand of dust suppression and sand-fixation in dusty areas and desertification environments, which opens a new application field for liquid mulching film with high safety and environmental protection.
33483018	3	34	from	effects	502:508	arg1	properties					560:569	mechanical properties	549:569	mechanical properties in dry and wet states	549:591	The effects of liquid mulching film composition on mechanical properties in dry and wet states were investigated.
33483018	1	35	theme	facile	200:205	arg1	process					226:232	a facile secondary spraying process	198:232	a facile secondary spraying process in this work	198:245	An eco-friendly dust suppression and sand-fixation liquid mulching film was prepared via a facile secondary spraying process in this work.
33483018	2	36	theme	cationic	392:399	arg1	starch					401:406	cationic starch	392:406	cationic starch (CS) / sodium lignosulfonate (LS) film	392:445	Water polyurethane (WPU) was blended with dissolved humic acid (HA) firstly, and then the blend solutions (HWPU) were sprayed on the surface of cationic starch (CS) / sodium lignosulfonate (LS) film to synthesize the liquid mulching film (CLS-HWPU).
33483018	2	36	theme	cationic	392:399	arg1	CS					409:410	CS	409:410	CS	409:410	Water polyurethane (WPU) was blended with dissolved humic acid (HA) firstly, and then the blend solutions (HWPU) were sprayed on the surface of cationic starch (CS) / sodium lignosulfonate (LS) film to synthesize the liquid mulching film (CLS-HWPU).
33483018	6	37	theme	UV	1114:1115	arg1	resistance					1117:1126	good UV resistance	1109:1126	good UV resistance	1109:1126	The results indicated that the CLS-HWPU film had good UV resistance, thermal stability, anti-erosion, and biodegradation.
33483018	5	38	theme	light	819:823	arg1	transmittance					825:837	light transmittance	819:837	light transmittance	819:837	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	4	39	theme	film	679:682	arg1	%					690:690	3%	689:690	3% (CS)	689:695	The results showed that the optimal composition of liquid mulching film was: 3% (CS), 0.9 % (LS), 1.5 % (glycerol), 2% (HA), and 30 % (WPU).
33483018	4	39	theme	film	679:682	arg1	composition					648:658	the optimal composition	636:658	the optimal composition of liquid mulching film	636:682	The results showed that the optimal composition of liquid mulching film was: 3% (CS), 0.9 % (LS), 1.5 % (glycerol), 2% (HA), and 30 % (WPU).
33483018	3	40	theme	liquid	513:518	arg1	composition					534:544	liquid mulching film composition	513:544	liquid mulching film composition	513:544	The effects of liquid mulching film composition on mechanical properties in dry and wet states were investigated.
33483018	1	41	theme	secondary	207:215	arg1	process					226:232	a facile secondary spraying process	198:232	a facile secondary spraying process in this work	198:245	An eco-friendly dust suppression and sand-fixation liquid mulching film was prepared via a facile secondary spraying process in this work.
33483018	6	42	theme	good	1109:1112	arg1	resistance					1117:1126	good UV resistance	1109:1126	good UV resistance	1109:1126	The results indicated that the CLS-HWPU film had good UV resistance, thermal stability, anti-erosion, and biodegradation.
33483018	3	43	theme	dry	574:576	arg1	states					586:591	dry and wet states	574:591	dry and wet states	574:591	The effects of liquid mulching film composition on mechanical properties in dry and wet states were investigated.
33483018	1	44	theme	eco-friendly	112:123	arg1	suppression					130:140	An eco-friendly dust suppression	109:140	An eco-friendly dust suppression	109:140	An eco-friendly dust suppression and sand-fixation liquid mulching film was prepared via a facile secondary spraying process in this work.
33483018	5	45	theme	resistance	1043:1052	arg1	terms					810:814	terms	810:814	terms of light transmittance	810:837	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	5	45	theme	resistance	1043:1052	arg1	test					1054:1057	erosion resistance test	1035:1057	erosion resistance test	1035:1057	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	1	46	theme	spraying	217:224	arg1	process					226:232	a facile secondary spraying process	198:232	a facile secondary spraying process in this work	198:245	An eco-friendly dust suppression and sand-fixation liquid mulching film was prepared via a facile secondary spraying process in this work.
33483018	0	47	theme	eco-friendly	39:50	arg1	suppression					57:67	an eco-friendly dust suppression	36:67	an eco-friendly dust suppression	36:67	Preparation and characterization of an eco-friendly dust suppression and sand-fixation liquid mulching film.
33483018	2	48	theme	mulching	472:479	arg1	film					481:484	the liquid mulching film	461:484	the liquid mulching film (CLS-HWPU)	461:495	Water polyurethane (WPU) was blended with dissolved humic acid (HA) firstly, and then the blend solutions (HWPU) were sprayed on the surface of cationic starch (CS) / sodium lignosulfonate (LS) film to synthesize the liquid mulching film (CLS-HWPU).
33483018	2	48	theme	mulching	472:479	arg1	CLS-HWPU					487:494	CLS-HWPU	487:494	CLS-HWPU	487:494	Water polyurethane (WPU) was blended with dissolved humic acid (HA) firstly, and then the blend solutions (HWPU) were sprayed on the surface of cationic starch (CS) / sodium lignosulfonate (LS) film to synthesize the liquid mulching film (CLS-HWPU).
33483018	7	49	theme	new	1318:1320	arg1	field					1334:1338	a new application field	1316:1338	a new application field for liquid mulching film with high safety and environmental protection	1316:1409	The CLS-HWPU film meets the demand of dust suppression and sand-fixation in dusty areas and desertification environments, which opens a new application field for liquid mulching film with high safety and environmental protection.
33483018	2	50	theme	Water	248:252	arg1	WPU					268:270	WPU	268:270	WPU	268:270	Water polyurethane (WPU) was blended with dissolved humic acid (HA) firstly, and then the blend solutions (HWPU) were sprayed on the surface of cationic starch (CS) / sodium lignosulfonate (LS) film to synthesize the liquid mulching film (CLS-HWPU).
33483018	2	50	theme	Water	248:252	arg1	polyurethane					254:265	Water polyurethane	248:265	Water polyurethane (WPU)	248:271	Water polyurethane (WPU) was blended with dissolved humic acid (HA) firstly, and then the blend solutions (HWPU) were sprayed on the surface of cationic starch (CS) / sodium lignosulfonate (LS) film to synthesize the liquid mulching film (CLS-HWPU).
33483018	7	51	from	sand-fixation	1241:1253	arg1	environments					1290:1301	desertification environments	1274:1301	desertification environments	1274:1301	The CLS-HWPU film meets the demand of dust suppression and sand-fixation in dusty areas and desertification environments, which opens a new application field for liquid mulching film with high safety and environmental protection.
33483018	7	51	from	sand-fixation	1241:1253	arg1	areas					1264:1268	dusty areas	1258:1268	dusty areas	1258:1268	The CLS-HWPU film meets the demand of dust suppression and sand-fixation in dusty areas and desertification environments, which opens a new application field for liquid mulching film with high safety and environmental protection.
33483018	7	52	theme	environmental	1386:1398	arg1	protection					1400:1409	environmental protection	1386:1409	environmental protection	1386:1409	The CLS-HWPU film meets the demand of dust suppression and sand-fixation in dusty areas and desertification environments, which opens a new application field for liquid mulching film with high safety and environmental protection.
33483018	2	53	theme	liquid	465:470	arg1	film					481:484	the liquid mulching film	461:484	the liquid mulching film (CLS-HWPU)	461:495	Water polyurethane (WPU) was blended with dissolved humic acid (HA) firstly, and then the blend solutions (HWPU) were sprayed on the surface of cationic starch (CS) / sodium lignosulfonate (LS) film to synthesize the liquid mulching film (CLS-HWPU).
33483018	2	53	theme	liquid	465:470	arg1	CLS-HWPU					487:494	CLS-HWPU	487:494	CLS-HWPU	487:494	Water polyurethane (WPU) was blended with dissolved humic acid (HA) firstly, and then the blend solutions (HWPU) were sprayed on the surface of cationic starch (CS) / sodium lignosulfonate (LS) film to synthesize the liquid mulching film (CLS-HWPU).
33483018	5	54	theme	contact	870:876	arg1	terms					810:814	terms	810:814	terms of light transmittance	810:837	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	5	54	theme	contact	870:876	arg1	test					884:887	contact angle test	870:887	contact angle test	870:887	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	3	55	theme	mulching	520:527	arg1	composition					534:544	liquid mulching film composition	513:544	liquid mulching film composition	513:544	The effects of liquid mulching film composition on mechanical properties in dry and wet states were investigated.
33483018	1	56	from	process	226:232	arg1	work					242:245	this work	237:245	this work	237:245	An eco-friendly dust suppression and sand-fixation liquid mulching film was prepared via a facile secondary spraying process in this work.
33483018	0	57	theme	suppression	57:67	arg1	characterization					16:31	characterization	16:31	characterization	16:31	Preparation and characterization of an eco-friendly dust suppression and sand-fixation liquid mulching film.
33483018	0	57	theme	suppression	57:67	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and characterization of an eco-friendly dust suppression and sand-fixation liquid mulching film.
33483018	5	58	theme	total	926:930	arg1	reflectance-Fourier					932:950	total reflectance-Fourier	926:950	total reflectance-Fourier transform infrared spectrometer (ATR-FTIR)	926:993	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	5	58	theme	total	926:930	arg1	terms					810:814	terms	810:814	terms of light transmittance	810:837	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	6	59	theme	CLS-HWPU	1091:1098	arg1	film					1100:1103	the CLS-HWPU film	1087:1103	the CLS-HWPU film	1087:1103	The results indicated that the CLS-HWPU film had good UV resistance, thermal stability, anti-erosion, and biodegradation.
33483018	5	60	theme	angle	878:882	arg1	terms					810:814	terms	810:814	terms of light transmittance	810:837	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	5	60	theme	angle	878:882	arg1	test					884:887	contact angle test	870:887	contact angle test	870:887	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	1	61	theme	dust	125:128	arg1	suppression					130:140	An eco-friendly dust suppression	109:140	An eco-friendly dust suppression	109:140	An eco-friendly dust suppression and sand-fixation liquid mulching film was prepared via a facile secondary spraying process in this work.
33483018	0	62	theme	dust	52:55	arg1	suppression					57:67	an eco-friendly dust suppression	36:67	an eco-friendly dust suppression	36:67	Preparation and characterization of an eco-friendly dust suppression and sand-fixation liquid mulching film.
33483018	5	63	theme	transmittance	825:837	arg1	test					864:867	degradation performance test	840:867	degradation performance test	840:867	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	5	63	theme	transmittance	825:837	arg1	test					884:887	contact angle test	870:887	contact angle test	870:887	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	5	63	theme	transmittance	825:837	arg1	analysis					1015:1022	thermo gravimetric analysis	996:1022	thermo gravimetric analysis (TGA)	996:1028	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	5	63	theme	transmittance	825:837	arg1	terms					810:814	terms	810:814	terms of light transmittance	810:837	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	5	63	theme	transmittance	825:837	arg1	test					1054:1057	erosion resistance test	1035:1057	erosion resistance test	1035:1057	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	5	63	theme	transmittance	825:837	arg1	reflectance-Fourier					932:950	total reflectance-Fourier	926:950	total reflectance-Fourier transform infrared spectrometer (ATR-FTIR)	926:993	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	5	63	theme	transmittance	825:837	arg1	microscopy					908:917	scanning electron microscopy	890:917	scanning electron microscopy (SEM)	890:923	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	1	64	theme	mulching	167:174	arg1	film					176:179	sand-fixation liquid mulching film	146:179	sand-fixation liquid mulching film	146:179	An eco-friendly dust suppression and sand-fixation liquid mulching film was prepared via a facile secondary spraying process in this work.
33483018	5	65	theme	mulching	773:780	arg1	films					782:786	The CLS-HWPU liquid mulching films	753:786	The CLS-HWPU liquid mulching films	753:786	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	6	66	contain	had	1105:1107	arg2	biodegradation					1166:1179	biodegradation	1166:1179	biodegradation	1166:1179	The results indicated that the CLS-HWPU film had good UV resistance, thermal stability, anti-erosion, and biodegradation.
33483018	6	66	contain	had	1105:1107	arg2	anti-erosion					1148:1159	anti-erosion	1148:1159	anti-erosion	1148:1159	The results indicated that the CLS-HWPU film had good UV resistance, thermal stability, anti-erosion, and biodegradation.
33483018	6	66	contain	had	1105:1107	arg2	stability					1137:1145	thermal stability	1129:1145	thermal stability	1129:1145	The results indicated that the CLS-HWPU film had good UV resistance, thermal stability, anti-erosion, and biodegradation.
33483018	6	66	contain	had	1105:1107	arg1	film					1100:1103	the CLS-HWPU film	1087:1103	the CLS-HWPU film	1087:1103	The results indicated that the CLS-HWPU film had good UV resistance, thermal stability, anti-erosion, and biodegradation.
33483018	6	66	contain	had	1105:1107	arg2	resistance					1117:1126	good UV resistance	1109:1126	good UV resistance	1109:1126	The results indicated that the CLS-HWPU film had good UV resistance, thermal stability, anti-erosion, and biodegradation.
33483018	3	67	theme	wet	582:584	arg1	states					586:591	dry and wet states	574:591	dry and wet states	574:591	The effects of liquid mulching film composition on mechanical properties in dry and wet states were investigated.
33483018	3	68	theme	mechanical	549:558	arg1	properties					560:569	mechanical properties	549:569	mechanical properties in dry and wet states	549:591	The effects of liquid mulching film composition on mechanical properties in dry and wet states were investigated.
33483018	4	69	theme	mulching	670:677	arg1	film					679:682	liquid mulching film	663:682	liquid mulching film	663:682	The results showed that the optimal composition of liquid mulching film was: 3% (CS), 0.9 % (LS), 1.5 % (glycerol), 2% (HA), and 30 % (WPU).
33483018	1	70	theme	sand-fixation	146:158	arg1	film					176:179	sand-fixation liquid mulching film	146:179	sand-fixation liquid mulching film	146:179	An eco-friendly dust suppression and sand-fixation liquid mulching film was prepared via a facile secondary spraying process in this work.
33483018	7	71	theme	high	1370:1373	arg1	safety					1375:1380	high safety	1370:1380	high safety	1370:1380	The CLS-HWPU film meets the demand of dust suppression and sand-fixation in dusty areas and desertification environments, which opens a new application field for liquid mulching film with high safety and environmental protection.
33483018	0	72	theme	sand-fixation	73:85	arg1	film					103:106	sand-fixation liquid mulching film	73:106	sand-fixation liquid mulching film	73:106	Preparation and characterization of an eco-friendly dust suppression and sand-fixation liquid mulching film.
33483018	5	73	theme	performance	852:862	arg1	terms					810:814	terms	810:814	terms of light transmittance	810:837	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	5	73	theme	performance	852:862	arg1	test					864:867	degradation performance test	840:867	degradation performance test	840:867	The CLS-HWPU liquid mulching films were characterized in terms of light transmittance, degradation performance test, contact angle test, scanning electron microscopy (SEM), total reflectance-Fourier transform infrared spectrometer (ATR-FTIR), thermo gravimetric analysis (TGA), and erosion resistance test.
33483018	3	74	theme	film	529:532	arg1	composition					534:544	liquid mulching film composition	513:544	liquid mulching film composition	513:544	The effects of liquid mulching film composition on mechanical properties in dry and wet states were investigated.
33483018	7	75	with	film	1360:1363	arg1	safety					1375:1380	high safety	1370:1380	high safety	1370:1380	The CLS-HWPU film meets the demand of dust suppression and sand-fixation in dusty areas and desertification environments, which opens a new application field for liquid mulching film with high safety and environmental protection.
33483018	7	75	with	film	1360:1363	arg1	protection					1400:1409	environmental protection	1386:1409	environmental protection	1386:1409	The CLS-HWPU film meets the demand of dust suppression and sand-fixation in dusty areas and desertification environments, which opens a new application field for liquid mulching film with high safety and environmental protection.
33483018	1	76	theme	liquid	160:165	arg1	film					176:179	sand-fixation liquid mulching film	146:179	sand-fixation liquid mulching film	146:179	An eco-friendly dust suppression and sand-fixation liquid mulching film was prepared via a facile secondary spraying process in this work.
33483018	7	77	theme	mulching	1351:1358	arg1	film					1360:1363	liquid mulching film	1344:1363	liquid mulching film with high safety and environmental protection	1344:1409	The CLS-HWPU film meets the demand of dust suppression and sand-fixation in dusty areas and desertification environments, which opens a new application field for liquid mulching film with high safety and environmental protection.
33483018	2	78	theme	LS	438:439	arg1	film					442:445	cationic starch (CS) / sodium lignosulfonate (LS) film	392:445	cationic starch (CS) / sodium lignosulfonate (LS) film	392:445	Water polyurethane (WPU) was blended with dissolved humic acid (HA) firstly, and then the blend solutions (HWPU) were sprayed on the surface of cationic starch (CS) / sodium lignosulfonate (LS) film to synthesize the liquid mulching film (CLS-HWPU).
33483018	7	79	theme	dusty	1258:1262	arg1	areas					1264:1268	dusty areas	1258:1268	dusty areas	1258:1268	The CLS-HWPU film meets the demand of dust suppression and sand-fixation in dusty areas and desertification environments, which opens a new application field for liquid mulching film with high safety and environmental protection.
33483018	3	80	theme	composition	534:544	arg1	effects					502:508	The effects	498:508	The effects of liquid mulching film composition on mechanical properties in dry and wet states	498:591	The effects of liquid mulching film composition on mechanical properties in dry and wet states were investigated.
33483018	2	81	theme	blend	338:342	arg1	HWPU					355:358	HWPU	355:358	HWPU	355:358	Water polyurethane (WPU) was blended with dissolved humic acid (HA) firstly, and then the blend solutions (HWPU) were sprayed on the surface of cationic starch (CS) / sodium lignosulfonate (LS) film to synthesize the liquid mulching film (CLS-HWPU).
33483018	2	81	theme	blend	338:342	arg1	solutions					344:352	then the blend solutions	329:352	then the blend solutions (HWPU)	329:359	Water polyurethane (WPU) was blended with dissolved humic acid (HA) firstly, and then the blend solutions (HWPU) were sprayed on the surface of cationic starch (CS) / sodium lignosulfonate (LS) film to synthesize the liquid mulching film (CLS-HWPU).
33483018	6	82	theme	thermal	1129:1135	arg1	stability					1137:1145	thermal stability	1129:1145	thermal stability	1129:1145	The results indicated that the CLS-HWPU film had good UV resistance, thermal stability, anti-erosion, and biodegradation.
33398399	13	0	theme	strain	1631:1636	arg1	TLY-12T					1638:1644	strain TLY-12T	1631:1644	strain TLY-12T	1631:1644	Based on the phylogenetic, phenotypic, chemotaxonomic and genome pairwise data, strain TLY-12T is considered to represent a novel species in a new genus in the family Promicromonosporaceae, for which the name Puerhibacterium puerhi gen. nov, sp.
33398399	9	1	theme	16S	964:966	arg1	rRNA					968:971	16S rRNA	964:971	16S rRNA gene	964:976	Phylogenetic analyses of 16S rRNA gene showed that this strain belonged to the family Promicromonosporaceae, and was most closely related to Isoptericola cucumis DSM 101603 T, which gave sequence similarity of 97.9%.
33398399	6	2	theme	unidentified	705:716	arg1	phospholipid					718:729	an unidentified phospholipid	702:729	an unidentified phospholipid (PL)	702:734	The polar lipids were phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidylinositol (PI), phosphoglycolipid (PGL), glycolipid (GL) and an unidentified phospholipid (PL).
33398399	6	2	theme	unidentified	705:716	arg1	PL					732:733	PL	732:733	PL	732:733	The polar lipids were phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidylinositol (PI), phosphoglycolipid (PGL), glycolipid (GL) and an unidentified phospholipid (PL).
33398399	13	3	theme	chemotaxonomic	1590:1603	arg1	data					1625:1628	the phylogenetic, phenotypic, chemotaxonomic and genome pairwise data	1560:1628	the phylogenetic, phenotypic, chemotaxonomic and genome pairwise data	1560:1628	Based on the phylogenetic, phenotypic, chemotaxonomic and genome pairwise data, strain TLY-12T is considered to represent a novel species in a new genus in the family Promicromonosporaceae, for which the name Puerhibacterium puerhi gen. nov, sp.
33398399	9	4	theme	gene	973:976	arg1	analyses					952:959	Phylogenetic analyses	939:959	Phylogenetic analyses of 16S rRNA gene	939:976	Phylogenetic analyses of 16S rRNA gene showed that this strain belonged to the family Promicromonosporaceae, and was most closely related to Isoptericola cucumis DSM 101603 T, which gave sequence similarity of 97.9%.
33398399	10	5	theme	3.91 Mbp	1200:1207	arg1	size					1192:1195	a genome size	1183:1195	a genome size of 3.91 Mbp	1183:1207	Genome sequencing revealed a genome size of 3.91 Mbp and a G + C content of 75.0%.
33398399	10	5	theme	3.91 Mbp	1200:1207	arg1	content					1221:1227	a G + C content	1213:1227	a G + C content of 75.0%	1213:1236	Genome sequencing revealed a genome size of 3.91 Mbp and a G + C content of 75.0%.
33398399	12	6	theme	Promicromonosporaceae	1528:1548	arg1	genera					1518:1523	other related genera	1504:1523	other related genera of Promicromonosporaceae	1504:1548	Genome phylogenetic analysis showed that strain TLY-12T formed a separate evolutionary branch, and was parallel to other related genera of Promicromonosporaceae.
33398399	13	7	theme	phylogenetic	1564:1575	arg1	data					1625:1628	the phylogenetic, phenotypic, chemotaxonomic and genome pairwise data	1560:1628	the phylogenetic, phenotypic, chemotaxonomic and genome pairwise data	1560:1628	Based on the phylogenetic, phenotypic, chemotaxonomic and genome pairwise data, strain TLY-12T is considered to represent a novel species in a new genus in the family Promicromonosporaceae, for which the name Puerhibacterium puerhi gen. nov, sp.
33398399	11	8	theme	species	1327:1333	arg1	threshold					1335:1343	the species threshold	1323:1343	the species threshold of described Promicromonosporaceae species	1323:1386	Average nucleotide identity and digital DNA-DNA hybridization values were all below the species threshold of described Promicromonosporaceae species.
33398399	12	9	theme	phylogenetic	1396:1407	arg1	analysis					1409:1416	Genome phylogenetic analysis	1389:1416	Genome phylogenetic analysis	1389:1416	Genome phylogenetic analysis showed that strain TLY-12T formed a separate evolutionary branch, and was parallel to other related genera of Promicromonosporaceae.
33398399	13	10	theme	name	1755:1758	arg1	sp					1793:1794	the name Puerhibacterium puerhi gen. nov, sp	1751:1794	sp	1793:1794	Based on the phylogenetic, phenotypic, chemotaxonomic and genome pairwise data, strain TLY-12T is considered to represent a novel species in a new genus in the family Promicromonosporaceae, for which the name Puerhibacterium puerhi gen. nov, sp.
33398399	13	11	theme	phenotypic	1578:1587	arg1	data					1625:1628	the phylogenetic, phenotypic, chemotaxonomic and genome pairwise data	1560:1628	the phylogenetic, phenotypic, chemotaxonomic and genome pairwise data	1560:1628	Based on the phylogenetic, phenotypic, chemotaxonomic and genome pairwise data, strain TLY-12T is considered to represent a novel species in a new genus in the family Promicromonosporaceae, for which the name Puerhibacterium puerhi gen. nov, sp.
33398399	7	12	contain	contained	755:763	arg2	lysine					807:812	lysine	807:812	lysine	807:812	The peptidoglycan contained glutamic acid, aspartic acid, alanine and lysine, with the last named being the diagnostic diamino acid.
33398399	7	12	contain	contained	755:763	arg1	peptidoglycan					741:753	The peptidoglycan	737:753	The peptidoglycan	737:753	The peptidoglycan contained glutamic acid, aspartic acid, alanine and lysine, with the last named being the diagnostic diamino acid.
33398399	7	12	contain	contained	755:763	arg2	alanine					795:801	alanine	795:801	alanine	795:801	The peptidoglycan contained glutamic acid, aspartic acid, alanine and lysine, with the last named being the diagnostic diamino acid.
33398399	7	12	contain	contained	755:763	arg2	acid					774:777	glutamic acid	765:777	glutamic acid	765:777	The peptidoglycan contained glutamic acid, aspartic acid, alanine and lysine, with the last named being the diagnostic diamino acid.
33398399	7	12	contain	contained	755:763	arg2	acid					789:792	aspartic acid	780:792	aspartic acid	780:792	The peptidoglycan contained glutamic acid, aspartic acid, alanine and lysine, with the last named being the diagnostic diamino acid.
33398399	7	13	theme	diagnostic	845:854	arg1	last					824:827	last	824:827	last	824:827	The peptidoglycan contained glutamic acid, aspartic acid, alanine and lysine, with the last named being the diagnostic diamino acid.
33398399	7	13	theme	diagnostic	845:854	arg1	acid					864:867	the diagnostic diamino acid	841:867	the diagnostic diamino acid	841:867	The peptidoglycan contained glutamic acid, aspartic acid, alanine and lysine, with the last named being the diagnostic diamino acid.
33398399	9	14	theme	family	1018:1023	arg1	Promicromonosporaceae					1025:1045	the family Promicromonosporaceae	1014:1045	the family Promicromonosporaceae	1014:1045	Phylogenetic analyses of 16S rRNA gene showed that this strain belonged to the family Promicromonosporaceae, and was most closely related to Isoptericola cucumis DSM 101603 T, which gave sequence similarity of 97.9%.
33398399	12	15	theme	related	1510:1516	arg1	genera					1518:1523	other related genera	1504:1523	other related genera of Promicromonosporaceae	1504:1548	Genome phylogenetic analysis showed that strain TLY-12T formed a separate evolutionary branch, and was parallel to other related genera of Promicromonosporaceae.
33398399	13	16	theme	Puerhibacterium	1760:1774	arg1	sp					1793:1794	the name Puerhibacterium puerhi gen. nov, sp	1751:1794	sp	1793:1794	Based on the phylogenetic, phenotypic, chemotaxonomic and genome pairwise data, strain TLY-12T is considered to represent a novel species in a new genus in the family Promicromonosporaceae, for which the name Puerhibacterium puerhi gen. nov, sp.
33398399	2	17	theme	pile-fermentation	237:253	arg1	process					255:261	the Pu-erh tea pile-fermentation process	222:261	the Pu-erh tea pile-fermentation process in Pu'er city, Yunnan, China	222:290	A Gram-staining positive aerobic bacterium, designated TLY-12T, was isolated from the Pu-erh tea pile-fermentation process in Pu'er city, Yunnan, China.
33398399	1	18	theme	Pu-erh	110:115	arg1	pile-fermentation					121:137	Pu-erh tea pile-fermentation	110:137	Pu-erh tea pile-fermentation	110:137	nov., a novel member of the family Promicromonosporaceae, isolated from Pu-erh tea pile-fermentation.
33398399	11	19	theme	nucleotide	1247:1256	arg1	identity					1258:1265	Average nucleotide identity	1239:1265	Average nucleotide identity	1239:1265	Average nucleotide identity and digital DNA-DNA hybridization values were all below the species threshold of described Promicromonosporaceae species.
33398399	2	20	theme	Pu-erh	226:231	arg1	process					255:261	the Pu-erh tea pile-fermentation process	222:261	the Pu-erh tea pile-fermentation process in Pu'er city, Yunnan, China	222:290	A Gram-staining positive aerobic bacterium, designated TLY-12T, was isolated from the Pu-erh tea pile-fermentation process in Pu'er city, Yunnan, China.
33398399	7	21	theme	aspartic	780:787	arg1	acid					789:792	aspartic acid	780:792	aspartic acid	780:792	The peptidoglycan contained glutamic acid, aspartic acid, alanine and lysine, with the last named being the diagnostic diamino acid.
33398399	3	22	dep	optimum	326:332	arg1	30 °C					335:339	30 °C	335:339	30 °C	335:339	Strain TLY-12T grew at 15-37 °C (optimum, 30 °C), pH 6.0-11.0 (optimum, pH 9.0) and 0-9.0% (w/v) NaCl (optimum, 3.0%).
33398399	13	23	theme	puerhi	1776:1781	arg1	sp					1793:1794	the name Puerhibacterium puerhi gen. nov, sp	1751:1794	sp	1793:1794	Based on the phylogenetic, phenotypic, chemotaxonomic and genome pairwise data, strain TLY-12T is considered to represent a novel species in a new genus in the family Promicromonosporaceae, for which the name Puerhibacterium puerhi gen. nov, sp.
33398399	11	24	theme	DNA-DNA	1279:1285	arg1	hybridization					1287:1299	digital DNA-DNA hybridization	1271:1299	digital DNA-DNA hybridization	1271:1299	Average nucleotide identity and digital DNA-DNA hybridization values were all below the species threshold of described Promicromonosporaceae species.
33398399	9	25	theme	cucumis	1093:1099	arg1	101603 T					1105:1112	Isoptericola cucumis DSM 101603 T	1080:1112	Isoptericola cucumis DSM 101603 T	1080:1112	Phylogenetic analyses of 16S rRNA gene showed that this strain belonged to the family Promicromonosporaceae, and was most closely related to Isoptericola cucumis DSM 101603 T, which gave sequence similarity of 97.9%.
33398399	13	26	from	genus	1698:1702	arg1	Promicromonosporaceae					1718:1738	the family Promicromonosporaceae	1707:1738	the family Promicromonosporaceae	1707:1738	Based on the phylogenetic, phenotypic, chemotaxonomic and genome pairwise data, strain TLY-12T is considered to represent a novel species in a new genus in the family Promicromonosporaceae, for which the name Puerhibacterium puerhi gen. nov, sp.
33398399	12	27	theme	evolutionary	1463:1474	arg1	branch					1476:1481	a separate evolutionary branch	1452:1481	a separate evolutionary branch	1452:1481	Genome phylogenetic analysis showed that strain TLY-12T formed a separate evolutionary branch, and was parallel to other related genera of Promicromonosporaceae.
33398399	2	28	attach	isolated	208:215	arg2	bacterium					173:181	A Gram-staining positive aerobic bacterium	140:181	A Gram-staining positive aerobic bacterium	140:181	A Gram-staining positive aerobic bacterium, designated TLY-12T, was isolated from the Pu-erh tea pile-fermentation process in Pu'er city, Yunnan, China.
33398399	2	28	attach	isolated	208:215	arg1	process					255:261	the Pu-erh tea pile-fermentation process	222:261	the Pu-erh tea pile-fermentation process in Pu'er city, Yunnan, China	222:290	A Gram-staining positive aerobic bacterium, designated TLY-12T, was isolated from the Pu-erh tea pile-fermentation process in Pu'er city, Yunnan, China.
33398399	11	29	theme	Average	1239:1245	arg1	identity					1258:1265	Average nucleotide identity	1239:1265	Average nucleotide identity	1239:1265	Average nucleotide identity and digital DNA-DNA hybridization values were all below the species threshold of described Promicromonosporaceae species.
33398399	0	30	theme	Puerhibacterium	0:14	arg1	nov.					28:31	Puerhibacterium puerhi gen. nov.	0:31	Puerhibacterium puerhi gen. nov.	0:31	Puerhibacterium puerhi gen. nov., sp.
33398399	3	31	dep	optimum	396:402	arg1	%					408:408	3.0%	405:408	3.0%	405:408	Strain TLY-12T grew at 15-37 °C (optimum, 30 °C), pH 6.0-11.0 (optimum, pH 9.0) and 0-9.0% (w/v) NaCl (optimum, 3.0%).
33398399	4	32	theme	major	416:420	arg1	anteiso-C15:0					448:460	anteiso-C15:0	448:460	anteiso-C15:0	448:460	The major cellular fatty acids were anteiso-C15:0, C16:0 and iso-C16:0.
33398399	4	32	theme	major	416:420	arg1	acids					437:441	The major cellular fatty acids	412:441	The major cellular fatty acids	412:441	The major cellular fatty acids were anteiso-C15:0, C16:0 and iso-C16:0.
33398399	13	33	theme	gen.	1783:1786	arg1	sp					1793:1794	the name Puerhibacterium puerhi gen. nov, sp	1751:1794	sp	1793:1794	Based on the phylogenetic, phenotypic, chemotaxonomic and genome pairwise data, strain TLY-12T is considered to represent a novel species in a new genus in the family Promicromonosporaceae, for which the name Puerhibacterium puerhi gen. nov, sp.
33398399	3	34	theme	0-9.0	377:381	arg1	%					382:382	%	382:382	%	382:382	Strain TLY-12T grew at 15-37 °C (optimum, 30 °C), pH 6.0-11.0 (optimum, pH 9.0) and 0-9.0% (w/v) NaCl (optimum, 3.0%).
33398399	0	35	theme	gen.	23:26	arg1	nov.					28:31	Puerhibacterium puerhi gen. nov.	0:31	Puerhibacterium puerhi gen. nov.	0:31	Puerhibacterium puerhi gen. nov., sp.
33398399	9	36	theme	sequence	1126:1133	arg1	similarity					1135:1144	sequence similarity	1126:1144	sequence similarity of 97.9%	1126:1153	Phylogenetic analyses of 16S rRNA gene showed that this strain belonged to the family Promicromonosporaceae, and was most closely related to Isoptericola cucumis DSM 101603 T, which gave sequence similarity of 97.9%.
33398399	1	37	theme	Promicromonosporaceae	73:93	arg1	member					52:57	a novel member	44:57	a novel member of the family Promicromonosporaceae	44:93	nov., a novel member of the family Promicromonosporaceae, isolated from Pu-erh tea pile-fermentation.
33398399	1	37	theme	Promicromonosporaceae	73:93	arg1	nov.					38:41	nov.	38:41	nov.	38:41	nov., a novel member of the family Promicromonosporaceae, isolated from Pu-erh tea pile-fermentation.
33398399	2	38	from	process	255:261	arg1	China					286:290	China	286:290	China	286:290	A Gram-staining positive aerobic bacterium, designated TLY-12T, was isolated from the Pu-erh tea pile-fermentation process in Pu'er city, Yunnan, China.
33398399	2	38	from	process	255:261	arg1	city					272:275	Pu'er city	266:275	Pu'er city	266:275	A Gram-staining positive aerobic bacterium, designated TLY-12T, was isolated from the Pu-erh tea pile-fermentation process in Pu'er city, Yunnan, China.
33398399	4	39	theme	fatty	431:435	arg1	anteiso-C15:0					448:460	anteiso-C15:0	448:460	anteiso-C15:0	448:460	The major cellular fatty acids were anteiso-C15:0, C16:0 and iso-C16:0.
33398399	4	39	theme	fatty	431:435	arg1	acids					437:441	The major cellular fatty acids	412:441	The major cellular fatty acids	412:441	The major cellular fatty acids were anteiso-C15:0, C16:0 and iso-C16:0.
33398399	2	40	theme	aerobic	165:171	arg1	bacterium					173:181	A Gram-staining positive aerobic bacterium	140:181	A Gram-staining positive aerobic bacterium	140:181	A Gram-staining positive aerobic bacterium, designated TLY-12T, was isolated from the Pu-erh tea pile-fermentation process in Pu'er city, Yunnan, China.
33398399	2	41	theme	Gram-staining	142:154	arg1	bacterium					173:181	A Gram-staining positive aerobic bacterium	140:181	A Gram-staining positive aerobic bacterium	140:181	A Gram-staining positive aerobic bacterium, designated TLY-12T, was isolated from the Pu-erh tea pile-fermentation process in Pu'er city, Yunnan, China.
33398399	9	42	theme	%	1153:1153	arg1	similarity					1135:1144	sequence similarity	1126:1144	sequence similarity of 97.9%	1126:1153	Phylogenetic analyses of 16S rRNA gene showed that this strain belonged to the family Promicromonosporaceae, and was most closely related to Isoptericola cucumis DSM 101603 T, which gave sequence similarity of 97.9%.
33398399	11	43	theme	species	1380:1386	arg1	threshold					1335:1343	the species threshold	1323:1343	the species threshold of described Promicromonosporaceae species	1323:1386	Average nucleotide identity and digital DNA-DNA hybridization values were all below the species threshold of described Promicromonosporaceae species.
33398399	13	44	from	species	1681:1687	arg1	genus					1698:1702	a new genus	1692:1702	a new genus in the family Promicromonosporaceae	1692:1738	Based on the phylogenetic, phenotypic, chemotaxonomic and genome pairwise data, strain TLY-12T is considered to represent a novel species in a new genus in the family Promicromonosporaceae, for which the name Puerhibacterium puerhi gen. nov, sp.
33398399	13	45	theme	pairwise	1616:1623	arg1	data					1625:1628	the phylogenetic, phenotypic, chemotaxonomic and genome pairwise data	1560:1628	the phylogenetic, phenotypic, chemotaxonomic and genome pairwise data	1560:1628	Based on the phylogenetic, phenotypic, chemotaxonomic and genome pairwise data, strain TLY-12T is considered to represent a novel species in a new genus in the family Promicromonosporaceae, for which the name Puerhibacterium puerhi gen. nov, sp.
33398399	9	46	theme	Phylogenetic	939:950	arg1	analyses					952:959	Phylogenetic analyses	939:959	Phylogenetic analyses of 16S rRNA gene	939:976	Phylogenetic analyses of 16S rRNA gene showed that this strain belonged to the family Promicromonosporaceae, and was most closely related to Isoptericola cucumis DSM 101603 T, which gave sequence similarity of 97.9%.
33398399	15	47	theme	type	1819:1822	arg1	strain					1824:1829	The type strain	1815:1829	The type strain	1815:1829	The type strain is TLY-12T (= CGMCC 1.17157T = KCTC 49467T).
33398399	15	47	theme	type	1819:1822	arg1	TLY-12T					1834:1840	TLY-12T	1834:1840	TLY-12T (= CGMCC 1.17157T = KCTC 49467T)	1834:1873	The type strain is TLY-12T (= CGMCC 1.17157T = KCTC 49467T).
33398399	3	48	dep	%	382:382	arg1	w/v					385:387	w/v	385:387	w/v	385:387	Strain TLY-12T grew at 15-37 °C (optimum, 30 °C), pH 6.0-11.0 (optimum, pH 9.0) and 0-9.0% (w/v) NaCl (optimum, 3.0%).
33398399	9	49	theme	rRNA	968:971	arg1	gene					973:976	16S rRNA gene	964:976	16S rRNA gene	964:976	Phylogenetic analyses of 16S rRNA gene showed that this strain belonged to the family Promicromonosporaceae, and was most closely related to Isoptericola cucumis DSM 101603 T, which gave sequence similarity of 97.9%.
33398399	2	50	theme	er	269:270	arg1	China					286:290	China	286:290	China	286:290	A Gram-staining positive aerobic bacterium, designated TLY-12T, was isolated from the Pu-erh tea pile-fermentation process in Pu'er city, Yunnan, China.
33398399	2	50	theme	er	269:270	arg1	city					272:275	Pu'er city	266:275	Pu'er city	266:275	A Gram-staining positive aerobic bacterium, designated TLY-12T, was isolated from the Pu-erh tea pile-fermentation process in Pu'er city, Yunnan, China.
33398399	5	51	theme	respiratory	488:498	arg1	quinone					500:506	The respiratory quinone	484:506	The respiratory quinone	484:506	The respiratory quinone were menaquinones MK-9 (H2) and MK-9 (H4).
33398399	5	51	theme	respiratory	488:498	arg1	MK-9					526:529	MK-9	526:529	MK-9	526:529	The respiratory quinone were menaquinones MK-9 (H2) and MK-9 (H4).
33398399	10	52	theme	Genome	1156:1161	arg1	sequencing					1163:1172	Genome sequencing	1156:1172	Genome sequencing	1156:1172	Genome sequencing revealed a genome size of 3.91 Mbp and a G + C content of 75.0%.
33398399	2	53	theme	Pu	266:267	arg1	China					286:290	China	286:290	China	286:290	A Gram-staining positive aerobic bacterium, designated TLY-12T, was isolated from the Pu-erh tea pile-fermentation process in Pu'er city, Yunnan, China.
33398399	2	53	theme	Pu	266:267	arg1	city					272:275	Pu'er city	266:275	Pu'er city	266:275	A Gram-staining positive aerobic bacterium, designated TLY-12T, was isolated from the Pu-erh tea pile-fermentation process in Pu'er city, Yunnan, China.
33398399	11	54	theme	described	1348:1356	arg1	species					1380:1386	described Promicromonosporaceae species	1348:1386	described Promicromonosporaceae species	1348:1386	Average nucleotide identity and digital DNA-DNA hybridization values were all below the species threshold of described Promicromonosporaceae species.
33398399	7	55	theme	diamino	856:862	arg1	last					824:827	last	824:827	last	824:827	The peptidoglycan contained glutamic acid, aspartic acid, alanine and lysine, with the last named being the diagnostic diamino acid.
33398399	7	55	theme	diamino	856:862	arg1	acid					864:867	the diagnostic diamino acid	841:867	the diagnostic diamino acid	841:867	The peptidoglycan contained glutamic acid, aspartic acid, alanine and lysine, with the last named being the diagnostic diamino acid.
33398399	10	56	theme	genome	1185:1190	arg1	size					1192:1195	a genome size	1183:1195	a genome size of 3.91 Mbp	1183:1207	Genome sequencing revealed a genome size of 3.91 Mbp and a G + C content of 75.0%.
33398399	13	57	theme	new	1694:1696	arg1	genus					1698:1702	a new genus	1692:1702	a new genus in the family Promicromonosporaceae	1692:1738	Based on the phylogenetic, phenotypic, chemotaxonomic and genome pairwise data, strain TLY-12T is considered to represent a novel species in a new genus in the family Promicromonosporaceae, for which the name Puerhibacterium puerhi gen. nov, sp.
33398399	12	58	theme	Genome	1389:1394	arg1	analysis					1409:1416	Genome phylogenetic analysis	1389:1416	Genome phylogenetic analysis	1389:1416	Genome phylogenetic analysis showed that strain TLY-12T formed a separate evolutionary branch, and was parallel to other related genera of Promicromonosporaceae.
33398399	11	59	theme	identity	1258:1265	arg1	values					1301:1306	Average nucleotide identity and digital DNA-DNA hybridization values	1239:1306	Average nucleotide identity and digital DNA-DNA hybridization values	1239:1306	Average nucleotide identity and digital DNA-DNA hybridization values were all below the species threshold of described Promicromonosporaceae species.
33398399	12	60	theme	other	1504:1508	arg1	genera					1518:1523	other related genera	1504:1523	other related genera of Promicromonosporaceae	1504:1548	Genome phylogenetic analysis showed that strain TLY-12T formed a separate evolutionary branch, and was parallel to other related genera of Promicromonosporaceae.
33398399	13	61	theme	family	1711:1716	arg1	Promicromonosporaceae					1718:1738	the family Promicromonosporaceae	1707:1738	the family Promicromonosporaceae	1707:1738	Based on the phylogenetic, phenotypic, chemotaxonomic and genome pairwise data, strain TLY-12T is considered to represent a novel species in a new genus in the family Promicromonosporaceae, for which the name Puerhibacterium puerhi gen. nov, sp.
33398399	2	62	theme	tea	233:235	arg1	process					255:261	the Pu-erh tea pile-fermentation process	222:261	the Pu-erh tea pile-fermentation process in Pu'er city, Yunnan, China	222:290	A Gram-staining positive aerobic bacterium, designated TLY-12T, was isolated from the Pu-erh tea pile-fermentation process in Pu'er city, Yunnan, China.
33398399	1	63	theme	tea	117:119	arg1	pile-fermentation					121:137	Pu-erh tea pile-fermentation	110:137	Pu-erh tea pile-fermentation	110:137	nov., a novel member of the family Promicromonosporaceae, isolated from Pu-erh tea pile-fermentation.
33398399	11	64	theme	hybridization	1287:1299	arg1	values					1301:1306	Average nucleotide identity and digital DNA-DNA hybridization values	1239:1306	Average nucleotide identity and digital DNA-DNA hybridization values	1239:1306	Average nucleotide identity and digital DNA-DNA hybridization values were all below the species threshold of described Promicromonosporaceae species.
33398399	10	65	theme	G + C	1215:1219	arg1	content					1221:1227	a G + C content	1213:1227	a G + C content of 75.0%	1213:1236	Genome sequencing revealed a genome size of 3.91 Mbp and a G + C content of 75.0%.
33398399	11	66	theme	digital	1271:1277	arg1	hybridization					1287:1299	digital DNA-DNA hybridization	1271:1299	digital DNA-DNA hybridization	1271:1299	Average nucleotide identity and digital DNA-DNA hybridization values were all below the species threshold of described Promicromonosporaceae species.
33398399	9	67	theme	Isoptericola	1080:1091	arg1	101603 T					1105:1112	Isoptericola cucumis DSM 101603 T	1080:1112	Isoptericola cucumis DSM 101603 T	1080:1112	Phylogenetic analyses of 16S rRNA gene showed that this strain belonged to the family Promicromonosporaceae, and was most closely related to Isoptericola cucumis DSM 101603 T, which gave sequence similarity of 97.9%.
33398399	15	68	theme	= CGMCC	1843:1849	arg1	49467T					1867:1872	= CGMCC 1.17157T = KCTC 49467T	1843:1872	= CGMCC 1.17157T = KCTC 49467T	1843:1872	The type strain is TLY-12T (= CGMCC 1.17157T = KCTC 49467T).
33398399	15	68	theme	= CGMCC	1843:1849	arg1	TLY-12T					1834:1840	TLY-12T	1834:1840	TLY-12T (= CGMCC 1.17157T = KCTC 49467T)	1834:1873	The type strain is TLY-12T (= CGMCC 1.17157T = KCTC 49467T).
33398399	15	69	theme	1.17157T = KCTC	1851:1865	arg1	49467T					1867:1872	= CGMCC 1.17157T = KCTC 49467T	1843:1872	= CGMCC 1.17157T = KCTC 49467T	1843:1872	The type strain is TLY-12T (= CGMCC 1.17157T = KCTC 49467T).
33398399	15	69	theme	1.17157T = KCTC	1851:1865	arg1	TLY-12T					1834:1840	TLY-12T	1834:1840	TLY-12T (= CGMCC 1.17157T = KCTC 49467T)	1834:1873	The type strain is TLY-12T (= CGMCC 1.17157T = KCTC 49467T).
33398399	6	70	theme	polar	555:559	arg1	phosphatidylglycerol					573:592	phosphatidylglycerol	573:592	phosphatidylglycerol (PG)	573:597	The polar lipids were phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidylinositol (PI), phosphoglycolipid (PGL), glycolipid (GL) and an unidentified phospholipid (PL).
33398399	6	70	theme	polar	555:559	arg1	lipids					561:566	The polar lipids	551:566	The polar lipids	551:566	The polar lipids were phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidylinositol (PI), phosphoglycolipid (PGL), glycolipid (GL) and an unidentified phospholipid (PL).
33398399	9	71	theme	DSM	1101:1103	arg1	101603 T					1105:1112	Isoptericola cucumis DSM 101603 T	1080:1112	Isoptericola cucumis DSM 101603 T	1080:1112	Phylogenetic analyses of 16S rRNA gene showed that this strain belonged to the family Promicromonosporaceae, and was most closely related to Isoptericola cucumis DSM 101603 T, which gave sequence similarity of 97.9%.
33398399	7	72	theme	glutamic	765:772	arg1	acid					774:777	glutamic acid	765:777	glutamic acid	765:777	The peptidoglycan contained glutamic acid, aspartic acid, alanine and lysine, with the last named being the diagnostic diamino acid.
33398399	1	73	theme	novel	46:50	arg1	member					52:57	a novel member	44:57	a novel member of the family Promicromonosporaceae	44:93	nov., a novel member of the family Promicromonosporaceae, isolated from Pu-erh tea pile-fermentation.
33398399	1	73	theme	novel	46:50	arg1	nov.					38:41	nov.	38:41	nov.	38:41	nov., a novel member of the family Promicromonosporaceae, isolated from Pu-erh tea pile-fermentation.
33398399	12	74	theme	separate	1454:1461	arg1	branch					1476:1481	a separate evolutionary branch	1452:1481	a separate evolutionary branch	1452:1481	Genome phylogenetic analysis showed that strain TLY-12T formed a separate evolutionary branch, and was parallel to other related genera of Promicromonosporaceae.
33398399	10	75	theme	%	1236:1236	arg1	size					1192:1195	a genome size	1183:1195	a genome size of 3.91 Mbp	1183:1207	Genome sequencing revealed a genome size of 3.91 Mbp and a G + C content of 75.0%.
33398399	10	75	theme	%	1236:1236	arg1	content					1221:1227	a G + C content	1213:1227	a G + C content of 75.0%	1213:1236	Genome sequencing revealed a genome size of 3.91 Mbp and a G + C content of 75.0%.
33398399	13	76	theme	novel	1675:1679	arg1	species					1681:1687	a novel species	1673:1687	a novel species	1673:1687	Based on the phylogenetic, phenotypic, chemotaxonomic and genome pairwise data, strain TLY-12T is considered to represent a novel species in a new genus in the family Promicromonosporaceae, for which the name Puerhibacterium puerhi gen. nov, sp.
33398399	0	77	theme	puerhi	16:21	arg1	nov.					28:31	Puerhibacterium puerhi gen. nov.	0:31	Puerhibacterium puerhi gen. nov.	0:31	Puerhibacterium puerhi gen. nov., sp.
33398399	4	78	theme	cellular	422:429	arg1	anteiso-C15:0					448:460	anteiso-C15:0	448:460	anteiso-C15:0	448:460	The major cellular fatty acids were anteiso-C15:0, C16:0 and iso-C16:0.
33398399	4	78	theme	cellular	422:429	arg1	acids					437:441	The major cellular fatty acids	412:441	The major cellular fatty acids	412:441	The major cellular fatty acids were anteiso-C15:0, C16:0 and iso-C16:0.
33398399	8	79	theme	Whole-cell	870:879	arg1	sugars					881:886	Whole-cell sugars	870:886	Whole-cell sugars of the isolate	870:901	Whole-cell sugars of the isolate were ribose, galactose and glucose.
33398399	1	80	theme	family	66:71	arg1	Promicromonosporaceae					73:93	the family Promicromonosporaceae	62:93	the family Promicromonosporaceae	62:93	nov., a novel member of the family Promicromonosporaceae, isolated from Pu-erh tea pile-fermentation.
33398399	12	81	theme	strain	1430:1435	arg1	TLY-12T					1437:1443	strain TLY-12T	1430:1443	strain TLY-12T	1430:1443	Genome phylogenetic analysis showed that strain TLY-12T formed a separate evolutionary branch, and was parallel to other related genera of Promicromonosporaceae.
33398399	3	82	theme	%	382:382	arg1	optimum					396:402	optimum	396:402	optimum	396:402	Strain TLY-12T grew at 15-37 °C (optimum, 30 °C), pH 6.0-11.0 (optimum, pH 9.0) and 0-9.0% (w/v) NaCl (optimum, 3.0%).
33398399	3	82	theme	%	382:382	arg1	NaCl					390:393	0-9.0% (w/v) NaCl	377:393	0-9.0% (w/v) NaCl (optimum, 3.0%)	377:409	Strain TLY-12T grew at 15-37 °C (optimum, 30 °C), pH 6.0-11.0 (optimum, pH 9.0) and 0-9.0% (w/v) NaCl (optimum, 3.0%).
33398399	3	83	dep	optimum	356:362	arg1	pH					365:366	pH 9.0	365:370	pH 9.0	365:370	Strain TLY-12T grew at 15-37 °C (optimum, 30 °C), pH 6.0-11.0 (optimum, pH 9.0) and 0-9.0% (w/v) NaCl (optimum, 3.0%).
33398399	2	84	theme	positive	156:163	arg1	bacterium					173:181	A Gram-staining positive aerobic bacterium	140:181	A Gram-staining positive aerobic bacterium	140:181	A Gram-staining positive aerobic bacterium, designated TLY-12T, was isolated from the Pu-erh tea pile-fermentation process in Pu'er city, Yunnan, China.
33398399	8	85	theme	isolate	895:901	arg1	sugars					881:886	Whole-cell sugars	870:886	Whole-cell sugars of the isolate	870:901	Whole-cell sugars of the isolate were ribose, galactose and glucose.
33398399	0	86	dep	sp	34:35	arg1	nov.					28:31	Puerhibacterium puerhi gen. nov.	0:31	Puerhibacterium puerhi gen. nov.	0:31	Puerhibacterium puerhi gen. nov., sp.
33398399	11	87	theme	Promicromonosporaceae	1358:1378	arg1	species					1380:1386	described Promicromonosporaceae species	1348:1386	described Promicromonosporaceae species	1348:1386	Average nucleotide identity and digital DNA-DNA hybridization values were all below the species threshold of described Promicromonosporaceae species.
33398399	13	88	theme	nov	1788:1790	arg1	sp					1793:1794	the name Puerhibacterium puerhi gen. nov, sp	1751:1794	sp	1793:1794	Based on the phylogenetic, phenotypic, chemotaxonomic and genome pairwise data, strain TLY-12T is considered to represent a novel species in a new genus in the family Promicromonosporaceae, for which the name Puerhibacterium puerhi gen. nov, sp.
32985644	5	0	theme	genomic	783:789	arg1	variation					791:799	genomic variation	783:799	genomic variation	783:799	Therefore, genomic variation, and/or mutations, as well as environmental and growth factors can change the composition of a fungal cell's biofilm.
32985644	3	1	theme	synthetic	499:507	arg1	surfaces					509:516	natural and synthetic surfaces	487:516	natural and synthetic surfaces	487:516	Biofilms represent the majority of known microbial communities, are ubiquitous, and are found on a multitude of natural and synthetic surfaces.
32985644	4	2	theme	biofilms	586:593	arg1	properties					564:573	in-turn nanomechanical properties	541:573	in-turn nanomechanical properties	541:573	The compositions, and in-turn nanomechanical properties, of fungal biofilms remain poorly understood, because these systems are complex, composed of anisotropic cellular and extracellular material, and importantly are species and environment dependent.
32985644	4	2	theme	biofilms	586:593	arg1	compositions					523:534	The compositions	519:534	The compositions	519:534	The compositions, and in-turn nanomechanical properties, of fungal biofilms remain poorly understood, because these systems are complex, composed of anisotropic cellular and extracellular material, and importantly are species and environment dependent.
32985644	0	3	theme	biofilms	87:94	arg1	mapping					45:51	nano-scale chemical and mechanical mapping	10:51	nano-scale chemical and mechanical mapping of antimicrobial-resistant fungal biofilms	10:94	Micro- to nano-scale chemical and mechanical mapping of antimicrobial-resistant fungal biofilms.
32985644	3	4	theme	surfaces	509:516	arg1	multitude					474:482	a multitude	472:482	a multitude of natural and synthetic surfaces	472:516	Biofilms represent the majority of known microbial communities, are ubiquitous, and are found on a multitude of natural and synthetic surfaces.
32985644	4	5	theme	fungal	579:584	arg1	biofilms					586:593	fungal biofilms	579:593	fungal biofilms	579:593	The compositions, and in-turn nanomechanical properties, of fungal biofilms remain poorly understood, because these systems are complex, composed of anisotropic cellular and extracellular material, and importantly are species and environment dependent.
32985644	9	6	theme	new	1280:1282	arg1	methodology					1297:1307	A new experimental methodology	1278:1307	A new experimental methodology of characterization	1278:1327	A new experimental methodology of characterization is proposed, employing a combination of atomic force microscopy (AFM), instrumented nanoindentation, and Synchrotron ATR-FTIR measurements.
32985644	7	7	dep	B-resistant	1231:1241	arg1	neoformans					1246:1255	C. neoformans	1243:1255	amphotericin B-resistant C. neoformans	1218:1255	neoformans) and amphotericin B-resistant C. neoformans (AmBRC.
32985644	5	8	theme	fungal	896:901	arg1	biofilm					910:916	a fungal cell's biofilm	894:916	a fungal cell's biofilm	894:916	Therefore, genomic variation, and/or mutations, as well as environmental and growth factors can change the composition of a fungal cell's biofilm.
32985644	6	9	dep	neoformans	1063:1072	arg1	neoformans					1078:1087	C. neoformans	1075:1087	C. neoformans	1075:1087	In this work, we probe the physico-mechanical and biochemical properties of two fungal species, Candida albicans (C. albicans) and Cryptococcus neoformans (C. neoformans), as well as two antifungal resistant sub-species of C. neoformans, fluconazole-resistant C. neoformans (FlucRC.
32985644	9	10	theme	instrumented	1400:1411	arg1	nanoindentation					1413:1427	instrumented nanoindentation	1400:1427	instrumented nanoindentation	1400:1427	A new experimental methodology of characterization is proposed, employing a combination of atomic force microscopy (AFM), instrumented nanoindentation, and Synchrotron ATR-FTIR measurements.
32985644	9	11	theme	experimental	1284:1295	arg1	methodology					1297:1307	A new experimental methodology	1278:1307	A new experimental methodology of characterization	1278:1327	A new experimental methodology of characterization is proposed, employing a combination of atomic force microscopy (AFM), instrumented nanoindentation, and Synchrotron ATR-FTIR measurements.
32985644	3	12	located	found	463:467	arg2	Biofilms					375:382	Biofilms	375:382	Biofilms	375:382	Biofilms represent the majority of known microbial communities, are ubiquitous, and are found on a multitude of natural and synthetic surfaces.
32985644	3	12	located	found	463:467	arg1	multitude					474:482	a multitude	472:482	a multitude of natural and synthetic surfaces	472:516	Biofilms represent the majority of known microbial communities, are ubiquitous, and are found on a multitude of natural and synthetic surfaces.
32985644	2	13	theme	surface	284:290	arg1	adhesion					292:299	strong surface adhesion	277:299	strong surface adhesion	277:299	The ECM is composed primarily of polysaccharides that facilitate strong surface adhesion, proliferation, and cellular protection from the surrounding environment.
32985644	5	14	theme	growth	849:854	arg1	factors					856:862	environmental and growth factors	831:862	factors	856:862	Therefore, genomic variation, and/or mutations, as well as environmental and growth factors can change the composition of a fungal cell's biofilm.
32985644	2	15	theme	strong	277:282	arg1	adhesion					292:299	strong surface adhesion	277:299	strong surface adhesion	277:299	The ECM is composed primarily of polysaccharides that facilitate strong surface adhesion, proliferation, and cellular protection from the surrounding environment.
32985644	4	16	theme	cellular	680:687	arg1	material					707:714	anisotropic cellular and extracellular material	668:714	anisotropic cellular and extracellular material	668:714	The compositions, and in-turn nanomechanical properties, of fungal biofilms remain poorly understood, because these systems are complex, composed of anisotropic cellular and extracellular material, and importantly are species and environment dependent.
32985644	6	17	dep	albicans	1023:1030	arg1	albicans					1036:1043	C. albicans	1033:1043	C. albicans	1033:1043	In this work, we probe the physico-mechanical and biochemical properties of two fungal species, Candida albicans (C. albicans) and Cryptococcus neoformans (C. neoformans), as well as two antifungal resistant sub-species of C. neoformans, fluconazole-resistant C. neoformans (FlucRC.
32985644	0	18	theme	mechanical	34:43	arg1	mapping					45:51	nano-scale chemical and mechanical mapping	10:51	nano-scale chemical and mechanical mapping of antimicrobial-resistant fungal biofilms	10:94	Micro- to nano-scale chemical and mechanical mapping of antimicrobial-resistant fungal biofilms.
32985644	9	19	theme	microscopy	1382:1391	arg1	combination					1354:1364	a combination	1352:1364	a combination of atomic force microscopy (AFM)	1352:1397	A new experimental methodology of characterization is proposed, employing a combination of atomic force microscopy (AFM), instrumented nanoindentation, and Synchrotron ATR-FTIR measurements.
32985644	9	19	theme	microscopy	1382:1391	arg1	measurements					1455:1466	Synchrotron ATR-FTIR measurements	1434:1466	Synchrotron ATR-FTIR measurements	1434:1466	A new experimental methodology of characterization is proposed, employing a combination of atomic force microscopy (AFM), instrumented nanoindentation, and Synchrotron ATR-FTIR measurements.
32985644	9	19	theme	microscopy	1382:1391	arg1	nanoindentation					1413:1427	instrumented nanoindentation	1400:1427	instrumented nanoindentation	1400:1427	A new experimental methodology of characterization is proposed, employing a combination of atomic force microscopy (AFM), instrumented nanoindentation, and Synchrotron ATR-FTIR measurements.
32985644	1	20	theme	polymeric	174:182	arg1	ECM					206:208	ECM	206:208	ECM	206:208	A fungal biofilm refers to the agglomeration of fungal cells surrounded by a polymeric extracellular matrix (ECM).
32985644	1	20	theme	polymeric	174:182	arg1	matrix					198:203	a polymeric extracellular matrix	172:203	a polymeric extracellular matrix (ECM)	172:209	A fungal biofilm refers to the agglomeration of fungal cells surrounded by a polymeric extracellular matrix (ECM).
32985644	9	21	theme	characterization	1312:1327	arg1	methodology					1297:1307	A new experimental methodology	1278:1307	A new experimental methodology of characterization	1278:1327	A new experimental methodology of characterization is proposed, employing a combination of atomic force microscopy (AFM), instrumented nanoindentation, and Synchrotron ATR-FTIR measurements.
32985644	1	22	theme	extracellular	184:196	arg1	ECM					206:208	ECM	206:208	ECM	206:208	A fungal biofilm refers to the agglomeration of fungal cells surrounded by a polymeric extracellular matrix (ECM).
32985644	1	22	theme	extracellular	184:196	arg1	matrix					198:203	a polymeric extracellular matrix	172:203	a polymeric extracellular matrix (ECM)	172:209	A fungal biofilm refers to the agglomeration of fungal cells surrounded by a polymeric extracellular matrix (ECM).
32985644	6	23	theme	biochemical	969:979	arg1	properties					981:990	the physico-mechanical and biochemical properties	942:990	the physico-mechanical and biochemical properties of two fungal species, Candida albicans (C. albicans) and Cryptococcus neoformans (C. neoformans), as well as two antifungal resistant sub-species of C. neoformans, fluconazole-resistant C. neoformans	942:1191	In this work, we probe the physico-mechanical and biochemical properties of two fungal species, Candida albicans (C. albicans) and Cryptococcus neoformans (C. neoformans), as well as two antifungal resistant sub-species of C. neoformans, fluconazole-resistant C. neoformans (FlucRC.
32985644	1	24	theme	fungal	99:104	arg1	biofilm					106:112	A fungal biofilm	97:112	A fungal biofilm	97:112	A fungal biofilm refers to the agglomeration of fungal cells surrounded by a polymeric extracellular matrix (ECM).
32985644	6	25	theme	fungal	999:1004	arg1	neoformans					1063:1072	Cryptococcus neoformans	1050:1072	Cryptococcus neoformans (C. neoformans)	1050:1088	In this work, we probe the physico-mechanical and biochemical properties of two fungal species, Candida albicans (C. albicans) and Cryptococcus neoformans (C. neoformans), as well as two antifungal resistant sub-species of C. neoformans, fluconazole-resistant C. neoformans (FlucRC.
32985644	6	25	theme	fungal	999:1004	arg1	species					1006:1012	two fungal species	995:1012	two fungal species	995:1012	In this work, we probe the physico-mechanical and biochemical properties of two fungal species, Candida albicans (C. albicans) and Cryptococcus neoformans (C. neoformans), as well as two antifungal resistant sub-species of C. neoformans, fluconazole-resistant C. neoformans (FlucRC.
32985644	6	25	theme	fungal	999:1004	arg1	albicans					1023:1030	Candida albicans	1015:1030	Candida albicans (C. albicans)	1015:1044	In this work, we probe the physico-mechanical and biochemical properties of two fungal species, Candida albicans (C. albicans) and Cryptococcus neoformans (C. neoformans), as well as two antifungal resistant sub-species of C. neoformans, fluconazole-resistant C. neoformans (FlucRC.
32985644	4	26	theme	anisotropic	668:678	arg1	material					707:714	anisotropic cellular and extracellular material	668:714	anisotropic cellular and extracellular material	668:714	The compositions, and in-turn nanomechanical properties, of fungal biofilms remain poorly understood, because these systems are complex, composed of anisotropic cellular and extracellular material, and importantly are species and environment dependent.
32985644	0	27	theme	chemical	21:28	arg1	mapping					45:51	nano-scale chemical and mechanical mapping	10:51	nano-scale chemical and mechanical mapping of antimicrobial-resistant fungal biofilms	10:94	Micro- to nano-scale chemical and mechanical mapping of antimicrobial-resistant fungal biofilms.
32985644	4	28	theme	nanomechanical	549:562	arg1	properties					564:573	in-turn nanomechanical properties	541:573	in-turn nanomechanical properties	541:573	The compositions, and in-turn nanomechanical properties, of fungal biofilms remain poorly understood, because these systems are complex, composed of anisotropic cellular and extracellular material, and importantly are species and environment dependent.
32985644	10	29	theme	chemical	1506:1513	arg1	characterisation					1515:1530	the nano-mechanical and chemical characterisation	1482:1530	the nano-mechanical and chemical characterisation of each fungal biofilm	1482:1553	This allowed the nano-mechanical and chemical characterisation of each fungal biofilm.
32985644	0	30	theme	nano-scale	10:19	arg1	mapping					45:51	nano-scale chemical and mechanical mapping	10:51	nano-scale chemical and mechanical mapping of antimicrobial-resistant fungal biofilms	10:94	Micro- to nano-scale chemical and mechanical mapping of antimicrobial-resistant fungal biofilms.
32985644	6	31	theme	physico-mechanical	946:963	arg1	properties					981:990	the physico-mechanical and biochemical properties	942:990	the physico-mechanical and biochemical properties of two fungal species, Candida albicans (C. albicans) and Cryptococcus neoformans (C. neoformans), as well as two antifungal resistant sub-species of C. neoformans, fluconazole-resistant C. neoformans	942:1191	In this work, we probe the physico-mechanical and biochemical properties of two fungal species, Candida albicans (C. albicans) and Cryptococcus neoformans (C. neoformans), as well as two antifungal resistant sub-species of C. neoformans, fluconazole-resistant C. neoformans (FlucRC.
32985644	7	32	dep	neoformans	1202:1211	arg1	AmBRC					1258:1262	AmBRC	1258:1262	AmBRC	1258:1262	neoformans) and amphotericin B-resistant C. neoformans (AmBRC.
32985644	2	33	theme	surrounding	350:360	arg1	environment					362:372	the surrounding environment	346:372	the surrounding environment	346:372	The ECM is composed primarily of polysaccharides that facilitate strong surface adhesion, proliferation, and cellular protection from the surrounding environment.
32985644	4	34	theme	extracellular	693:705	arg1	material					707:714	anisotropic cellular and extracellular material	668:714	anisotropic cellular and extracellular material	668:714	The compositions, and in-turn nanomechanical properties, of fungal biofilms remain poorly understood, because these systems are complex, composed of anisotropic cellular and extracellular material, and importantly are species and environment dependent.
32985644	3	35	theme	communities	426:436	arg1	majority					398:405	the majority	394:405	the majority of known microbial communities	394:436	Biofilms represent the majority of known microbial communities, are ubiquitous, and are found on a multitude of natural and synthetic surfaces.
32985644	10	36	theme	nano-mechanical	1486:1500	arg1	characterisation					1515:1530	the nano-mechanical and chemical characterisation	1482:1530	the nano-mechanical and chemical characterisation of each fungal biofilm	1482:1553	This allowed the nano-mechanical and chemical characterisation of each fungal biofilm.
32985644	9	37	theme	ATR-FTIR	1446:1453	arg1	measurements					1455:1466	Synchrotron ATR-FTIR measurements	1434:1466	Synchrotron ATR-FTIR measurements	1434:1466	A new experimental methodology of characterization is proposed, employing a combination of atomic force microscopy (AFM), instrumented nanoindentation, and Synchrotron ATR-FTIR measurements.
32985644	6	38	dep	probe	936:940	arg1	FlucRC					1194:1199	FlucRC	1194:1199	FlucRC	1194:1199	In this work, we probe the physico-mechanical and biochemical properties of two fungal species, Candida albicans (C. albicans) and Cryptococcus neoformans (C. neoformans), as well as two antifungal resistant sub-species of C. neoformans, fluconazole-resistant C. neoformans (FlucRC.
32985644	6	39	theme	Cryptococcus	1050:1061	arg1	neoformans					1063:1072	Cryptococcus neoformans	1050:1072	Cryptococcus neoformans (C. neoformans)	1050:1088	In this work, we probe the physico-mechanical and biochemical properties of two fungal species, Candida albicans (C. albicans) and Cryptococcus neoformans (C. neoformans), as well as two antifungal resistant sub-species of C. neoformans, fluconazole-resistant C. neoformans (FlucRC.
32985644	6	39	theme	Cryptococcus	1050:1061	arg1	species					1006:1012	two fungal species	995:1012	two fungal species	995:1012	In this work, we probe the physico-mechanical and biochemical properties of two fungal species, Candida albicans (C. albicans) and Cryptococcus neoformans (C. neoformans), as well as two antifungal resistant sub-species of C. neoformans, fluconazole-resistant C. neoformans (FlucRC.
32985644	3	40	theme	known	410:414	arg1	communities					426:436	known microbial communities	410:436	known microbial communities	410:436	Biofilms represent the majority of known microbial communities, are ubiquitous, and are found on a multitude of natural and synthetic surfaces.
32985644	10	41	theme	fungal	1540:1545	arg1	biofilm					1547:1553	each fungal biofilm	1535:1553	each fungal biofilm	1535:1553	This allowed the nano-mechanical and chemical characterisation of each fungal biofilm.
32985644	3	42	theme	natural	487:493	arg1	surfaces					509:516	natural and synthetic surfaces	487:516	natural and synthetic surfaces	487:516	Biofilms represent the majority of known microbial communities, are ubiquitous, and are found on a multitude of natural and synthetic surfaces.
32985644	3	43	theme	microbial	416:424	arg1	communities					426:436	known microbial communities	410:436	known microbial communities	410:436	Biofilms represent the majority of known microbial communities, are ubiquitous, and are found on a multitude of natural and synthetic surfaces.
32985644	4	44	theme	in-turn	541:547	arg1	properties					564:573	in-turn nanomechanical properties	541:573	in-turn nanomechanical properties	541:573	The compositions, and in-turn nanomechanical properties, of fungal biofilms remain poorly understood, because these systems are complex, composed of anisotropic cellular and extracellular material, and importantly are species and environment dependent.
32985644	9	45	theme	atomic	1369:1374	arg1	AFM					1394:1396	AFM	1394:1396	AFM	1394:1396	A new experimental methodology of characterization is proposed, employing a combination of atomic force microscopy (AFM), instrumented nanoindentation, and Synchrotron ATR-FTIR measurements.
32985644	9	45	theme	atomic	1369:1374	arg1	microscopy					1382:1391	atomic force microscopy	1369:1391	atomic force microscopy (AFM)	1369:1397	A new experimental methodology of characterization is proposed, employing a combination of atomic force microscopy (AFM), instrumented nanoindentation, and Synchrotron ATR-FTIR measurements.
32985644	9	46	theme	force	1376:1380	arg1	AFM					1394:1396	AFM	1394:1396	AFM	1394:1396	A new experimental methodology of characterization is proposed, employing a combination of atomic force microscopy (AFM), instrumented nanoindentation, and Synchrotron ATR-FTIR measurements.
32985644	9	46	theme	force	1376:1380	arg1	microscopy					1382:1391	atomic force microscopy	1369:1391	atomic force microscopy (AFM)	1369:1397	A new experimental methodology of characterization is proposed, employing a combination of atomic force microscopy (AFM), instrumented nanoindentation, and Synchrotron ATR-FTIR measurements.
32985644	6	47	dep	C.	1142:1143	arg1	neoformans					1145:1154	neoformans	1145:1154	neoformans	1145:1154	In this work, we probe the physico-mechanical and biochemical properties of two fungal species, Candida albicans (C. albicans) and Cryptococcus neoformans (C. neoformans), as well as two antifungal resistant sub-species of C. neoformans, fluconazole-resistant C. neoformans (FlucRC.
32985644	6	47	dep	C.	1142:1143	arg1	fluconazole-resistant					1157:1177	fluconazole-resistant	1157:1177	fluconazole-resistant	1157:1177	In this work, we probe the physico-mechanical and biochemical properties of two fungal species, Candida albicans (C. albicans) and Cryptococcus neoformans (C. neoformans), as well as two antifungal resistant sub-species of C. neoformans, fluconazole-resistant C. neoformans (FlucRC.
32985644	2	48	theme	cellular	321:328	arg1	protection					330:339	cellular protection	321:339	cellular protection	321:339	The ECM is composed primarily of polysaccharides that facilitate strong surface adhesion, proliferation, and cellular protection from the surrounding environment.
32985644	6	49	theme	Candida	1015:1021	arg1	species					1006:1012	two fungal species	995:1012	two fungal species	995:1012	In this work, we probe the physico-mechanical and biochemical properties of two fungal species, Candida albicans (C. albicans) and Cryptococcus neoformans (C. neoformans), as well as two antifungal resistant sub-species of C. neoformans, fluconazole-resistant C. neoformans (FlucRC.
32985644	6	49	theme	Candida	1015:1021	arg1	albicans					1023:1030	Candida albicans	1015:1030	Candida albicans (C. albicans)	1015:1044	In this work, we probe the physico-mechanical and biochemical properties of two fungal species, Candida albicans (C. albicans) and Cryptococcus neoformans (C. neoformans), as well as two antifungal resistant sub-species of C. neoformans, fluconazole-resistant C. neoformans (FlucRC.
32985644	0	50	theme	fungal	80:85	arg1	biofilms					87:94	antimicrobial-resistant fungal biofilms	56:94	antimicrobial-resistant fungal biofilms	56:94	Micro- to nano-scale chemical and mechanical mapping of antimicrobial-resistant fungal biofilms.
32985644	6	51	theme	neoformans	1182:1191	arg1	sub-species					1127:1137	sub-species	1127:1137	sub-species	1127:1137	In this work, we probe the physico-mechanical and biochemical properties of two fungal species, Candida albicans (C. albicans) and Cryptococcus neoformans (C. neoformans), as well as two antifungal resistant sub-species of C. neoformans, fluconazole-resistant C. neoformans (FlucRC.
32985644	1	52	theme	fungal	145:150	arg1	cells					152:156	fungal cells	145:156	fungal cells surrounded by a polymeric extracellular matrix (ECM)	145:209	A fungal biofilm refers to the agglomeration of fungal cells surrounded by a polymeric extracellular matrix (ECM).
32985644	10	53	theme	biofilm	1547:1553	arg1	characterisation					1515:1530	the nano-mechanical and chemical characterisation	1482:1530	the nano-mechanical and chemical characterisation of each fungal biofilm	1482:1553	This allowed the nano-mechanical and chemical characterisation of each fungal biofilm.
32985644	0	54	theme	antimicrobial-resistant	56:78	arg1	biofilms					87:94	antimicrobial-resistant fungal biofilms	56:94	antimicrobial-resistant fungal biofilms	56:94	Micro- to nano-scale chemical and mechanical mapping of antimicrobial-resistant fungal biofilms.
32985644	9	55	theme	Synchrotron	1434:1444	arg1	measurements					1455:1466	Synchrotron ATR-FTIR measurements	1434:1466	Synchrotron ATR-FTIR measurements	1434:1466	A new experimental methodology of characterization is proposed, employing a combination of atomic force microscopy (AFM), instrumented nanoindentation, and Synchrotron ATR-FTIR measurements.
32985644	5	56	theme	environmental	831:843	arg1	factors					856:862	environmental and growth factors	831:862	factors	856:862	Therefore, genomic variation, and/or mutations, as well as environmental and growth factors can change the composition of a fungal cell's biofilm.
32985644	1	57	theme	cells	152:156	arg1	agglomeration					128:140	the agglomeration	124:140	the agglomeration of fungal cells surrounded by a polymeric extracellular matrix (ECM)	124:209	A fungal biofilm refers to the agglomeration of fungal cells surrounded by a polymeric extracellular matrix (ECM).
32985644	7	58	theme	amphotericin	1218:1229	arg1	B-resistant					1231:1241	amphotericin B-resistant	1218:1241	amphotericin B-resistant C. neoformans	1218:1255	neoformans) and amphotericin B-resistant C. neoformans (AmBRC.
32985644	6	59	theme	species	1006:1012	arg1	properties					981:990	the physico-mechanical and biochemical properties	942:990	the physico-mechanical and biochemical properties of two fungal species, Candida albicans (C. albicans) and Cryptococcus neoformans (C. neoformans), as well as two antifungal resistant sub-species of C. neoformans, fluconazole-resistant C. neoformans	942:1191	In this work, we probe the physico-mechanical and biochemical properties of two fungal species, Candida albicans (C. albicans) and Cryptococcus neoformans (C. neoformans), as well as two antifungal resistant sub-species of C. neoformans, fluconazole-resistant C. neoformans (FlucRC.
32315798	0	0	theme	Dictyosphaerium	98:112	arg1	sp					114:115	Dictyosphaerium sp	98:115	Dictyosphaerium sp	98:115	Enhanced excretion of extracellular polymeric substances associated with nonylphenol tolerance in Dictyosphaerium sp.
32315798	3	1	from	composition	396:406	arg1	algae					455:459	algae	455:459	algae exposed to NP	455:473	In this study, a batch of algal culture experiments were carried out to elucidate the underlying mechanisms by investigating the production and composition of extracellular polymeric substances (EPS) in algae exposed to NP.
32315798	6	2	theme	excitation	695:704	arg1	analyses					747:754	The three-dimensional excitation and emission matrix fluorescence spectra analyses	673:754	The three-dimensional excitation and emission matrix fluorescence spectra analyses	673:754	The three-dimensional excitation and emission matrix fluorescence spectra analyses indicated that tyrosine- and tryptophan-like substances were the main functional compositions in the proteins of EPS.
32315798	5	3	theme	bound	631:635	arg1	EPS					637:639	bound EPS	631:639	bound EPS	631:639	The polysaccharides in soluble EPS and the proteins in bound EPS were specifically overproduced.
32315798	4	4	dep	enhanced	515:522	arg1	P < 0.001					525:533	P < 0.001	525:533	P < 0.001	525:533	The excretion of EPS was significantly enhanced (P < 0.001) in algae exposed to 4 and 8 mg/L of NP.
32315798	7	5	theme	cell	1055:1058	arg1	rate					1067:1070	cell growth rate	1055:1070	cell growth rate	1055:1070	In addition, enhanced EPS secretion significantly alleviated the toxicity of NP to the algae by the reduction of cell internalization, as indicated by the higher IC50, biomass, and cell growth rate in the algae with EPS.
32315798	6	6	theme	main	821:824	arg1	compositions					837:848	the main functional compositions	817:848	the main functional compositions in the proteins of EPS	817:871	The three-dimensional excitation and emission matrix fluorescence spectra analyses indicated that tyrosine- and tryptophan-like substances were the main functional compositions in the proteins of EPS.
32315798	6	6	theme	main	821:824	arg1	substances					801:810	tyrosine- and tryptophan-like substances	771:810	tyrosine- and tryptophan-like substances	771:810	The three-dimensional excitation and emission matrix fluorescence spectra analyses indicated that tyrosine- and tryptophan-like substances were the main functional compositions in the proteins of EPS.
32315798	8	7	theme	electron	1202:1209	arg1	microscopy					1211:1220	scanning electron microscopy	1193:1220	scanning electron microscopy	1193:1220	These discoveries along with the characterizations by algal cell surface hydrophobicity analysis, scanning electron microscopy, and Fourier transform infrared spectra spectroscopy demonstrated the vital role of EPS in the algal resistance to NP.
32315798	7	8	from	biomass	1042:1048	arg1	algae					1079:1083	the algae	1075:1083	the algae with EPS	1075:1092	In addition, enhanced EPS secretion significantly alleviated the toxicity of NP to the algae by the reduction of cell internalization, as indicated by the higher IC50, biomass, and cell growth rate in the algae with EPS.
32315798	3	9	theme	underlying	338:347	arg1	mechanisms					349:358	the underlying mechanisms	334:358	the underlying mechanisms	334:358	In this study, a batch of algal culture experiments were carried out to elucidate the underlying mechanisms by investigating the production and composition of extracellular polymeric substances (EPS) in algae exposed to NP.
32315798	4	10	theme	EPS	493:495	arg1	excretion					480:488	The excretion	476:488	The excretion of EPS	476:495	The excretion of EPS was significantly enhanced (P < 0.001) in algae exposed to 4 and 8 mg/L of NP.
32315798	3	11	dep	production	381:390	arg1	the					377:379	the	377:379	the	377:379	In this study, a batch of algal culture experiments were carried out to elucidate the underlying mechanisms by investigating the production and composition of extracellular polymeric substances (EPS) in algae exposed to NP.
32315798	8	12	theme	algal	1317:1321	arg1	resistance					1323:1332	the algal resistance	1313:1332	the algal resistance to NP	1313:1338	These discoveries along with the characterizations by algal cell surface hydrophobicity analysis, scanning electron microscopy, and Fourier transform infrared spectra spectroscopy demonstrated the vital role of EPS in the algal resistance to NP.
32315798	6	13	theme	three-dimensional	677:693	arg1	analyses					747:754	The three-dimensional excitation and emission matrix fluorescence spectra analyses	673:754	The three-dimensional excitation and emission matrix fluorescence spectra analyses	673:754	The three-dimensional excitation and emission matrix fluorescence spectra analyses indicated that tyrosine- and tryptophan-like substances were the main functional compositions in the proteins of EPS.
32315798	7	14	theme	enhanced	887:894	arg1	secretion					900:908	enhanced EPS secretion	887:908	enhanced EPS secretion	887:908	In addition, enhanced EPS secretion significantly alleviated the toxicity of NP to the algae by the reduction of cell internalization, as indicated by the higher IC50, biomass, and cell growth rate in the algae with EPS.
32315798	6	15	from	compositions	837:848	arg1	proteins					857:864	the proteins	853:864	the proteins of EPS	853:871	The three-dimensional excitation and emission matrix fluorescence spectra analyses indicated that tyrosine- and tryptophan-like substances were the main functional compositions in the proteins of EPS.
32315798	6	16	theme	tryptophan-like	785:799	arg1	compositions					837:848	the main functional compositions	817:848	the main functional compositions in the proteins of EPS	817:871	The three-dimensional excitation and emission matrix fluorescence spectra analyses indicated that tyrosine- and tryptophan-like substances were the main functional compositions in the proteins of EPS.
32315798	6	16	theme	tryptophan-like	785:799	arg1	substances					801:810	tyrosine- and tryptophan-like substances	771:810	tyrosine- and tryptophan-like substances	771:810	The three-dimensional excitation and emission matrix fluorescence spectra analyses indicated that tyrosine- and tryptophan-like substances were the main functional compositions in the proteins of EPS.
32315798	8	17	theme	algal	1149:1153	arg1	analysis					1183:1190	algal cell surface hydrophobicity analysis	1149:1190	algal cell surface hydrophobicity analysis	1149:1190	These discoveries along with the characterizations by algal cell surface hydrophobicity analysis, scanning electron microscopy, and Fourier transform infrared spectra spectroscopy demonstrated the vital role of EPS in the algal resistance to NP.
32315798	8	18	theme	cell	1155:1158	arg1	analysis					1183:1190	algal cell surface hydrophobicity analysis	1149:1190	algal cell surface hydrophobicity analysis	1149:1190	These discoveries along with the characterizations by algal cell surface hydrophobicity analysis, scanning electron microscopy, and Fourier transform infrared spectra spectroscopy demonstrated the vital role of EPS in the algal resistance to NP.
32315798	3	19	from	production	381:390	arg1	algae					455:459	algae	455:459	algae exposed to NP	455:473	In this study, a batch of algal culture experiments were carried out to elucidate the underlying mechanisms by investigating the production and composition of extracellular polymeric substances (EPS) in algae exposed to NP.
32315798	5	20	theme	soluble	599:605	arg1	EPS					607:609	soluble EPS	599:609	soluble EPS	599:609	The polysaccharides in soluble EPS and the proteins in bound EPS were specifically overproduced.
32315798	7	21	theme	NP	951:952	arg1	toxicity					939:946	the toxicity	935:946	the toxicity of NP to the algae	935:965	In addition, enhanced EPS secretion significantly alleviated the toxicity of NP to the algae by the reduction of cell internalization, as indicated by the higher IC50, biomass, and cell growth rate in the algae with EPS.
32315798	8	22	dep	Fourier	1227:1233	arg1	transform					1235:1243	transform	1235:1243	transform infrared spectra spectroscopy	1235:1273	These discoveries along with the characterizations by algal cell surface hydrophobicity analysis, scanning electron microscopy, and Fourier transform infrared spectra spectroscopy demonstrated the vital role of EPS in the algal resistance to NP.
32315798	3	23	theme	polymeric	425:433	arg1	EPS					447:449	EPS	447:449	EPS	447:449	In this study, a batch of algal culture experiments were carried out to elucidate the underlying mechanisms by investigating the production and composition of extracellular polymeric substances (EPS) in algae exposed to NP.
32315798	3	23	theme	polymeric	425:433	arg1	substances					435:444	extracellular polymeric substances	411:444	extracellular polymeric substances (EPS)	411:450	In this study, a batch of algal culture experiments were carried out to elucidate the underlying mechanisms by investigating the production and composition of extracellular polymeric substances (EPS) in algae exposed to NP.
32315798	0	24	theme	Enhanced	0:7	arg1	excretion					9:17	Enhanced excretion	0:17	Enhanced excretion of extracellular polymeric substances	0:55	Enhanced excretion of extracellular polymeric substances associated with nonylphenol tolerance in Dictyosphaerium sp.
32315798	7	25	theme	growth	1060:1065	arg1	rate					1067:1070	cell growth rate	1055:1070	cell growth rate	1055:1070	In addition, enhanced EPS secretion significantly alleviated the toxicity of NP to the algae by the reduction of cell internalization, as indicated by the higher IC50, biomass, and cell growth rate in the algae with EPS.
32315798	6	26	theme	tyrosine-	771:779	arg1	compositions					837:848	the main functional compositions	817:848	the main functional compositions in the proteins of EPS	817:871	The three-dimensional excitation and emission matrix fluorescence spectra analyses indicated that tyrosine- and tryptophan-like substances were the main functional compositions in the proteins of EPS.
32315798	6	26	theme	tyrosine-	771:779	arg1	substances					801:810	tyrosine- and tryptophan-like substances	771:810	tyrosine- and tryptophan-like substances	771:810	The three-dimensional excitation and emission matrix fluorescence spectra analyses indicated that tyrosine- and tryptophan-like substances were the main functional compositions in the proteins of EPS.
32315798	3	27	theme	substances	435:444	arg1	composition					396:406	composition	396:406	composition	396:406	In this study, a batch of algal culture experiments were carried out to elucidate the underlying mechanisms by investigating the production and composition of extracellular polymeric substances (EPS) in algae exposed to NP.
32315798	3	27	theme	substances	435:444	arg1	production					381:390	production	381:390	production	381:390	In this study, a batch of algal culture experiments were carried out to elucidate the underlying mechanisms by investigating the production and composition of extracellular polymeric substances (EPS) in algae exposed to NP.
32315798	0	28	theme	extracellular	22:34	arg1	substances					46:55	extracellular polymeric substances	22:55	extracellular polymeric substances	22:55	Enhanced excretion of extracellular polymeric substances associated with nonylphenol tolerance in Dictyosphaerium sp.
32315798	1	29	theme	Dictyosphaerium	118:132	arg1	sp					134:135	Dictyosphaerium sp	118:135	Dictyosphaerium sp.	118:136	Dictyosphaerium sp.
32315798	8	30	from	role	1298:1301	arg1	resistance					1323:1332	the algal resistance	1313:1332	the algal resistance to NP	1313:1338	These discoveries along with the characterizations by algal cell surface hydrophobicity analysis, scanning electron microscopy, and Fourier transform infrared spectra spectroscopy demonstrated the vital role of EPS in the algal resistance to NP.
32315798	8	31	theme	infrared	1245:1252	arg1	spectroscopy					1262:1273	infrared spectra spectroscopy	1245:1273	infrared spectra spectroscopy	1245:1273	These discoveries along with the characterizations by algal cell surface hydrophobicity analysis, scanning electron microscopy, and Fourier transform infrared spectra spectroscopy demonstrated the vital role of EPS in the algal resistance to NP.
32315798	7	32	theme	cell	987:990	arg1	internalization					992:1006	cell internalization	987:1006	cell internalization	987:1006	In addition, enhanced EPS secretion significantly alleviated the toxicity of NP to the algae by the reduction of cell internalization, as indicated by the higher IC50, biomass, and cell growth rate in the algae with EPS.
32315798	5	33	from	polysaccharides	580:594	arg1	EPS					607:609	soluble EPS	599:609	soluble EPS	599:609	The polysaccharides in soluble EPS and the proteins in bound EPS were specifically overproduced.
32315798	5	33	from	polysaccharides	580:594	arg1	EPS					637:639	bound EPS	631:639	bound EPS	631:639	The polysaccharides in soluble EPS and the proteins in bound EPS were specifically overproduced.
32315798	7	34	theme	internalization	992:1006	arg1	reduction					974:982	the reduction	970:982	the reduction of cell internalization	970:1006	In addition, enhanced EPS secretion significantly alleviated the toxicity of NP to the algae by the reduction of cell internalization, as indicated by the higher IC50, biomass, and cell growth rate in the algae with EPS.
32315798	0	35	theme	substances	46:55	arg1	excretion					9:17	Enhanced excretion	0:17	Enhanced excretion of extracellular polymeric substances	0:55	Enhanced excretion of extracellular polymeric substances associated with nonylphenol tolerance in Dictyosphaerium sp.
32315798	6	36	theme	EPS	869:871	arg1	proteins					857:864	the proteins	853:864	the proteins of EPS	853:871	The three-dimensional excitation and emission matrix fluorescence spectra analyses indicated that tyrosine- and tryptophan-like substances were the main functional compositions in the proteins of EPS.
32315798	0	37	theme	polymeric	36:44	arg1	substances					46:55	extracellular polymeric substances	22:55	extracellular polymeric substances	22:55	Enhanced excretion of extracellular polymeric substances associated with nonylphenol tolerance in Dictyosphaerium sp.
32315798	5	38	from	proteins	619:626	arg1	EPS					607:609	soluble EPS	599:609	soluble EPS	599:609	The polysaccharides in soluble EPS and the proteins in bound EPS were specifically overproduced.
32315798	5	38	from	proteins	619:626	arg1	EPS					637:639	bound EPS	631:639	bound EPS	631:639	The polysaccharides in soluble EPS and the proteins in bound EPS were specifically overproduced.
32315798	3	39	theme	extracellular	411:423	arg1	EPS					447:449	EPS	447:449	EPS	447:449	In this study, a batch of algal culture experiments were carried out to elucidate the underlying mechanisms by investigating the production and composition of extracellular polymeric substances (EPS) in algae exposed to NP.
32315798	3	39	theme	extracellular	411:423	arg1	substances					435:444	extracellular polymeric substances	411:444	extracellular polymeric substances (EPS)	411:450	In this study, a batch of algal culture experiments were carried out to elucidate the underlying mechanisms by investigating the production and composition of extracellular polymeric substances (EPS) in algae exposed to NP.
32315798	0	40	from	tolerance	85:93	arg1	sp					114:115	Dictyosphaerium sp	98:115	Dictyosphaerium sp	98:115	Enhanced excretion of extracellular polymeric substances associated with nonylphenol tolerance in Dictyosphaerium sp.
32315798	8	41	theme	hydrophobicity	1168:1181	arg1	analysis					1183:1190	algal cell surface hydrophobicity analysis	1149:1190	algal cell surface hydrophobicity analysis	1149:1190	These discoveries along with the characterizations by algal cell surface hydrophobicity analysis, scanning electron microscopy, and Fourier transform infrared spectra spectroscopy demonstrated the vital role of EPS in the algal resistance to NP.
32315798	8	42	theme	spectra	1254:1260	arg1	spectroscopy					1262:1273	infrared spectra spectroscopy	1245:1273	infrared spectra spectroscopy	1245:1273	These discoveries along with the characterizations by algal cell surface hydrophobicity analysis, scanning electron microscopy, and Fourier transform infrared spectra spectroscopy demonstrated the vital role of EPS in the algal resistance to NP.
32315798	7	43	from	rate	1067:1070	arg1	algae					1079:1083	the algae	1075:1083	the algae with EPS	1075:1092	In addition, enhanced EPS secretion significantly alleviated the toxicity of NP to the algae by the reduction of cell internalization, as indicated by the higher IC50, biomass, and cell growth rate in the algae with EPS.
32315798	3	44	theme	culture	284:290	arg1	experiments					292:302	algal culture experiments	278:302	algal culture experiments	278:302	In this study, a batch of algal culture experiments were carried out to elucidate the underlying mechanisms by investigating the production and composition of extracellular polymeric substances (EPS) in algae exposed to NP.
32315798	2	45	theme	NP	227:228	arg1	tolerance					230:238	NP tolerance	227:238	NP tolerance	227:238	is tolerant to nonylphenol (NP); however, knowledge regarding the mechanisms involved in NP tolerance is limited.
32315798	8	46	theme	scanning	1193:1200	arg1	microscopy					1211:1220	scanning electron microscopy	1193:1220	scanning electron microscopy	1193:1220	These discoveries along with the characterizations by algal cell surface hydrophobicity analysis, scanning electron microscopy, and Fourier transform infrared spectra spectroscopy demonstrated the vital role of EPS in the algal resistance to NP.
32315798	3	47	theme	experiments	292:302	arg1	batch					269:273	a batch	267:273	a batch of algal culture experiments	267:302	In this study, a batch of algal culture experiments were carried out to elucidate the underlying mechanisms by investigating the production and composition of extracellular polymeric substances (EPS) in algae exposed to NP.
32315798	6	48	theme	spectra	739:745	arg1	analyses					747:754	The three-dimensional excitation and emission matrix fluorescence spectra analyses	673:754	The three-dimensional excitation and emission matrix fluorescence spectra analyses	673:754	The three-dimensional excitation and emission matrix fluorescence spectra analyses indicated that tyrosine- and tryptophan-like substances were the main functional compositions in the proteins of EPS.
32315798	6	49	theme	fluorescence	726:737	arg1	spectra					739:745	emission matrix fluorescence spectra	710:745	emission matrix fluorescence spectra	710:745	The three-dimensional excitation and emission matrix fluorescence spectra analyses indicated that tyrosine- and tryptophan-like substances were the main functional compositions in the proteins of EPS.
32315798	4	50	theme	NP	572:573	arg1	NP					572:573	NP	572:573	NP	572:573	The excretion of EPS was significantly enhanced (P < 0.001) in algae exposed to 4 and 8 mg/L of NP.
32315798	4	50	theme	NP	572:573	arg1	4					556:556	4	556:556	4	556:556	The excretion of EPS was significantly enhanced (P < 0.001) in algae exposed to 4 and 8 mg/L of NP.
32315798	3	51	theme	algal	278:282	arg1	experiments					292:302	algal culture experiments	278:302	algal culture experiments	278:302	In this study, a batch of algal culture experiments were carried out to elucidate the underlying mechanisms by investigating the production and composition of extracellular polymeric substances (EPS) in algae exposed to NP.
32315798	6	52	theme	matrix	719:724	arg1	spectra					739:745	emission matrix fluorescence spectra	710:745	emission matrix fluorescence spectra	710:745	The three-dimensional excitation and emission matrix fluorescence spectra analyses indicated that tyrosine- and tryptophan-like substances were the main functional compositions in the proteins of EPS.
32315798	8	53	theme	vital	1292:1296	arg1	role					1298:1301	the vital role	1288:1301	the vital role of EPS in the algal resistance to NP	1288:1338	These discoveries along with the characterizations by algal cell surface hydrophobicity analysis, scanning electron microscopy, and Fourier transform infrared spectra spectroscopy demonstrated the vital role of EPS in the algal resistance to NP.
32315798	7	54	theme	EPS	896:898	arg1	secretion					900:908	enhanced EPS secretion	887:908	enhanced EPS secretion	887:908	In addition, enhanced EPS secretion significantly alleviated the toxicity of NP to the algae by the reduction of cell internalization, as indicated by the higher IC50, biomass, and cell growth rate in the algae with EPS.
32315798	6	55	theme	functional	826:835	arg1	compositions					837:848	the main functional compositions	817:848	the main functional compositions in the proteins of EPS	817:871	The three-dimensional excitation and emission matrix fluorescence spectra analyses indicated that tyrosine- and tryptophan-like substances were the main functional compositions in the proteins of EPS.
32315798	6	55	theme	functional	826:835	arg1	substances					801:810	tyrosine- and tryptophan-like substances	771:810	tyrosine- and tryptophan-like substances	771:810	The three-dimensional excitation and emission matrix fluorescence spectra analyses indicated that tyrosine- and tryptophan-like substances were the main functional compositions in the proteins of EPS.
32315798	7	56	theme	higher	1029:1034	arg1	IC50					1036:1039	the higher IC50	1025:1039	the higher IC50	1025:1039	In addition, enhanced EPS secretion significantly alleviated the toxicity of NP to the algae by the reduction of cell internalization, as indicated by the higher IC50, biomass, and cell growth rate in the algae with EPS.
32315798	6	57	theme	emission	710:717	arg1	spectra					739:745	emission matrix fluorescence spectra	710:745	emission matrix fluorescence spectra	710:745	The three-dimensional excitation and emission matrix fluorescence spectra analyses indicated that tyrosine- and tryptophan-like substances were the main functional compositions in the proteins of EPS.
32315798	8	58	theme	EPS	1306:1308	arg1	role					1298:1301	the vital role	1288:1301	the vital role of EPS in the algal resistance to NP	1288:1338	These discoveries along with the characterizations by algal cell surface hydrophobicity analysis, scanning electron microscopy, and Fourier transform infrared spectra spectroscopy demonstrated the vital role of EPS in the algal resistance to NP.
32315798	0	59	theme	nonylphenol	73:83	arg1	tolerance					85:93	nonylphenol tolerance	73:93	nonylphenol tolerance in Dictyosphaerium sp	73:115	Enhanced excretion of extracellular polymeric substances associated with nonylphenol tolerance in Dictyosphaerium sp.
32315798	8	60	theme	surface	1160:1166	arg1	analysis					1183:1190	algal cell surface hydrophobicity analysis	1149:1190	algal cell surface hydrophobicity analysis	1149:1190	These discoveries along with the characterizations by algal cell surface hydrophobicity analysis, scanning electron microscopy, and Fourier transform infrared spectra spectroscopy demonstrated the vital role of EPS in the algal resistance to NP.
32315798	7	61	with	algae	1079:1083	arg1	EPS					1090:1092	EPS	1090:1092	EPS	1090:1092	In addition, enhanced EPS secretion significantly alleviated the toxicity of NP to the algae by the reduction of cell internalization, as indicated by the higher IC50, biomass, and cell growth rate in the algae with EPS.
32315798	7	62	from	IC50	1036:1039	arg1	algae					1079:1083	the algae	1075:1083	the algae with EPS	1075:1092	In addition, enhanced EPS secretion significantly alleviated the toxicity of NP to the algae by the reduction of cell internalization, as indicated by the higher IC50, biomass, and cell growth rate in the algae with EPS.
33423571	0	0	theme	Colon-targeted	99:112	arg1	delivery					114:121	possible Colon-targeted delivery	90:121	possible Colon-targeted delivery	90:121	Mucoadhesive chitosan and thiolated chitosan nanoparticles containing alpha mangostin for possible Colon-targeted delivery.
33423571	12	1	theme	controlled-release	1393:1410	arg1	system					1426:1431	a controlled-release drug delivery system	1391:1431	a controlled-release drug delivery system of α-mangostin to the colon	1391:1459	Therefore, the mucoadhesive TCS-based NPs could be a promising candidate for a controlled-release drug delivery system of α-mangostin to the colon.
33423571	5	2	theme	wash-off	658:665	arg1	method					667:672	the wash-off method	654:672	the wash-off method	654:672	In vitro mucoadhesive properties were examined by the wash-off method.
33423571	6	3	theme	colorectal	727:736	arg1	cells					745:749	colorectal cancer cells	727:749	colorectal cancer cells	727:749	In addition, the anti-tumour activity was tested on colorectal cancer cells.
33423571	0	4	theme	possible	90:97	arg1	delivery					114:121	possible Colon-targeted delivery	90:121	possible Colon-targeted delivery	90:121	Mucoadhesive chitosan and thiolated chitosan nanoparticles containing alpha mangostin for possible Colon-targeted delivery.
33423571	10	5	theme	TCS-based	1145:1153	arg1	NPs					1155:1157	The TCS-based NPs	1141:1157	The TCS-based NPs with GP and L100	1141:1174	The TCS-based NPs with GP and L100 exhibited strong mucoadhesion to colon mucosa, more than uncoated-NPs and CS-based NPs.
33423571	5	6	theme	In	604:605	arg1	properties					626:635	In vitro mucoadhesive properties	604:635	In vitro mucoadhesive properties	604:635	In vitro mucoadhesive properties were examined by the wash-off method.
33423571	1	7	theme	colon-targeted	194:207	arg1	delivery					214:221	colon-targeted drug delivery	194:221	colon-targeted drug delivery	194:221	α-Mangostin-loaded mucoadhesive nanoparticles (NPs) were prepared for colon-targeted drug delivery against colorectal cancer cells using pH-dependent composite mucoadhesive NPs.
33423571	10	8	theme	colon	1209:1213	arg1	mucosa					1215:1220	colon mucosa	1209:1220	colon mucosa	1209:1220	The TCS-based NPs with GP and L100 exhibited strong mucoadhesion to colon mucosa, more than uncoated-NPs and CS-based NPs.
33423571	12	9	theme	delivery	1417:1424	arg1	system					1426:1431	a controlled-release drug delivery system	1391:1431	a controlled-release drug delivery system of α-mangostin to the colon	1391:1459	Therefore, the mucoadhesive TCS-based NPs could be a promising candidate for a controlled-release drug delivery system of α-mangostin to the colon.
33423571	8	10	theme	L100	941:944	arg1	process					946:952	GP and L100 process	934:952	GP and L100 process	934:952	The small size of NPs was found with TCS and larger NPs were observed by GP and L100 process.
33423571	8	11	theme	GP	934:935	arg1	process					946:952	GP and L100 process	934:952	GP and L100 process	934:952	The small size of NPs was found with TCS and larger NPs were observed by GP and L100 process.
33423571	7	12	from	oblong	794:799	arg1	shape					804:808	shape	804:808	shape	804:808	The results showed that NPs were slightly oblong in shape with particle size ranging between 300 and 900 nm.
33423571	5	13	theme	mucoadhesive	613:624	arg1	properties					626:635	In vitro mucoadhesive properties	604:635	In vitro mucoadhesive properties	604:635	In vitro mucoadhesive properties were examined by the wash-off method.
33423571	12	14	theme	mucoadhesive	1329:1340	arg1	candidate					1377:1385	a promising candidate	1365:1385	a promising candidate for a controlled-release drug delivery system of α-mangostin to the colon	1365:1459	Therefore, the mucoadhesive TCS-based NPs could be a promising candidate for a controlled-release drug delivery system of α-mangostin to the colon.
33423571	12	14	theme	mucoadhesive	1329:1340	arg1	NPs					1352:1354	the mucoadhesive TCS-based NPs	1325:1354	the mucoadhesive TCS-based NPs	1325:1354	Therefore, the mucoadhesive TCS-based NPs could be a promising candidate for a controlled-release drug delivery system of α-mangostin to the colon.
33423571	12	15	theme	promising	1367:1375	arg1	candidate					1377:1385	a promising candidate	1365:1385	a promising candidate for a controlled-release drug delivery system of α-mangostin to the colon	1365:1459	Therefore, the mucoadhesive TCS-based NPs could be a promising candidate for a controlled-release drug delivery system of α-mangostin to the colon.
33423571	12	15	theme	promising	1367:1375	arg1	NPs					1352:1354	the mucoadhesive TCS-based NPs	1325:1354	the mucoadhesive TCS-based NPs	1325:1354	Therefore, the mucoadhesive TCS-based NPs could be a promising candidate for a controlled-release drug delivery system of α-mangostin to the colon.
33423571	7	16	with	oblong	794:799	arg1	size					824:827	particle size	815:827	particle size ranging between 300 and 900 nm	815:858	The results showed that NPs were slightly oblong in shape with particle size ranging between 300 and 900 nm.
33423571	2	17	theme	thiolated	320:328	arg1	chitosan					330:337	thiolated chitosan	320:337	thiolated chitosan (TCS)	320:343	Chitosan (CS) and thiolated chitosan (TCS) were used to form the NPs, following by genipin (GP) crosslinking and the surface modification by Eudragit® L100 (L100).
33423571	2	17	theme	thiolated	320:328	arg1	TCS					340:342	TCS	340:342	TCS	340:342	Chitosan (CS) and thiolated chitosan (TCS) were used to form the NPs, following by genipin (GP) crosslinking and the surface modification by Eudragit® L100 (L100).
33423571	8	18	theme	small	865:869	arg1	size					871:874	The small size	861:874	The small size of NPs	861:881	The small size of NPs was found with TCS and larger NPs were observed by GP and L100 process.
33423571	1	19	theme	drug	209:212	arg1	delivery					214:221	colon-targeted drug delivery	194:221	colon-targeted drug delivery	194:221	α-Mangostin-loaded mucoadhesive nanoparticles (NPs) were prepared for colon-targeted drug delivery against colorectal cancer cells using pH-dependent composite mucoadhesive NPs.
33423571	7	20	from	shape	804:808	arg1	oblong					794:799	oblong	794:799	oblong	794:799	The results showed that NPs were slightly oblong in shape with particle size ranging between 300 and 900 nm.
33423571	8	21	theme	NPs	879:881	arg1	size					871:874	The small size	861:874	The small size of NPs	861:881	The small size of NPs was found with TCS and larger NPs were observed by GP and L100 process.
33423571	9	22	from	increase	988:995	arg1	loading					1012:1018	α-mangostin loading	1000:1018	α-mangostin loading	1000:1018	However, GP and L100 provided an increase in α-mangostin loading, limited the release of α-mangostin in the upper gastrointestinal tract, and enhanced α-mangostin delivery to the colon.
33423571	9	23	theme	α-mangostin	1044:1054	arg1	delivery					1118:1125	enhanced α-mangostin delivery	1097:1125	enhanced α-mangostin delivery to the colon	1097:1138	However, GP and L100 provided an increase in α-mangostin loading, limited the release of α-mangostin in the upper gastrointestinal tract, and enhanced α-mangostin delivery to the colon.
33423571	9	23	theme	α-mangostin	1044:1054	arg1	release					1033:1039	the release	1029:1039	the release of α-mangostin in the upper gastrointestinal tract	1029:1090	However, GP and L100 provided an increase in α-mangostin loading, limited the release of α-mangostin in the upper gastrointestinal tract, and enhanced α-mangostin delivery to the colon.
33423571	2	24	theme	GP	394:395	arg1	crosslinking					398:409	by genipin (GP) crosslinking	382:409	by genipin (GP) crosslinking	382:409	Chitosan (CS) and thiolated chitosan (TCS) were used to form the NPs, following by genipin (GP) crosslinking and the surface modification by Eudragit® L100 (L100).
33423571	0	25	theme	Mucoadhesive	0:11	arg1	chitosan					13:20	Mucoadhesive chitosan	0:20	Mucoadhesive chitosan	0:20	Mucoadhesive chitosan and thiolated chitosan nanoparticles containing alpha mangostin for possible Colon-targeted delivery.
33423571	10	26	with	NPs	1155:1157	arg1	L100					1171:1174	L100	1171:1174	L100	1171:1174	The TCS-based NPs with GP and L100 exhibited strong mucoadhesion to colon mucosa, more than uncoated-NPs and CS-based NPs.
33423571	10	26	with	NPs	1155:1157	arg1	GP					1164:1165	GP	1164:1165	GP	1164:1165	The TCS-based NPs with GP and L100 exhibited strong mucoadhesion to colon mucosa, more than uncoated-NPs and CS-based NPs.
33423571	6	27	theme	anti-tumour	692:702	arg1	activity					704:711	the anti-tumour activity	688:711	the anti-tumour activity	688:711	In addition, the anti-tumour activity was tested on colorectal cancer cells.
33423571	1	28	theme	colorectal	231:240	arg1	cells					249:253	colorectal cancer cells	231:253	colorectal cancer cells using pH-dependent composite mucoadhesive NPs	231:299	α-Mangostin-loaded mucoadhesive nanoparticles (NPs) were prepared for colon-targeted drug delivery against colorectal cancer cells using pH-dependent composite mucoadhesive NPs.
33423571	0	29	theme	thiolated	26:34	arg1	chitosan					36:43	thiolated chitosan	26:43	thiolated chitosan	26:43	Mucoadhesive chitosan and thiolated chitosan nanoparticles containing alpha mangostin for possible Colon-targeted delivery.
33423571	4	30	theme	α-mangostin	544:554	arg1	loading					556:562	The α-mangostin loading and release patterns	540:583	loading	556:562	The α-mangostin loading and release patterns were investigated.
33423571	1	31	theme	α-Mangostin-loaded	124:141	arg1	NPs					171:173	NPs	171:173	NPs	171:173	α-Mangostin-loaded mucoadhesive nanoparticles (NPs) were prepared for colon-targeted drug delivery against colorectal cancer cells using pH-dependent composite mucoadhesive NPs.
33423571	1	31	theme	α-Mangostin-loaded	124:141	arg1	nanoparticles					156:168	α-Mangostin-loaded mucoadhesive nanoparticles	124:168	α-Mangostin-loaded mucoadhesive nanoparticles (NPs)	124:174	α-Mangostin-loaded mucoadhesive nanoparticles (NPs) were prepared for colon-targeted drug delivery against colorectal cancer cells using pH-dependent composite mucoadhesive NPs.
33423571	1	32	theme	cancer	242:247	arg1	cells					249:253	colorectal cancer cells	231:253	colorectal cancer cells using pH-dependent composite mucoadhesive NPs	231:299	α-Mangostin-loaded mucoadhesive nanoparticles (NPs) were prepared for colon-targeted drug delivery against colorectal cancer cells using pH-dependent composite mucoadhesive NPs.
33423571	4	33	theme	release	568:574	arg1	patterns					576:583	The α-mangostin loading and release patterns	540:583	patterns	576:583	The α-mangostin loading and release patterns were investigated.
33423571	1	34	theme	mucoadhesive	143:154	arg1	NPs					171:173	NPs	171:173	NPs	171:173	α-Mangostin-loaded mucoadhesive nanoparticles (NPs) were prepared for colon-targeted drug delivery against colorectal cancer cells using pH-dependent composite mucoadhesive NPs.
33423571	1	34	theme	mucoadhesive	143:154	arg1	nanoparticles					156:168	α-Mangostin-loaded mucoadhesive nanoparticles	124:168	α-Mangostin-loaded mucoadhesive nanoparticles (NPs)	124:174	α-Mangostin-loaded mucoadhesive nanoparticles (NPs) were prepared for colon-targeted drug delivery against colorectal cancer cells using pH-dependent composite mucoadhesive NPs.
33423571	9	35	theme	upper	1063:1067	arg1	tract					1086:1090	the upper gastrointestinal tract	1059:1090	the upper gastrointestinal tract	1059:1090	However, GP and L100 provided an increase in α-mangostin loading, limited the release of α-mangostin in the upper gastrointestinal tract, and enhanced α-mangostin delivery to the colon.
33423571	2	36	theme	surface	419:425	arg1	modification					427:438	the surface modification	415:438	the surface modification by Eudragit® L100 (L100)	415:463	Chitosan (CS) and thiolated chitosan (TCS) were used to form the NPs, following by genipin (GP) crosslinking and the surface modification by Eudragit® L100 (L100).
33423571	10	37	theme	uncoated-NPs	1233:1244	arg1	NPs					1259:1261	uncoated-NPs and CS-based NPs	1233:1261	uncoated-NPs and CS-based NPs	1233:1261	The TCS-based NPs with GP and L100 exhibited strong mucoadhesion to colon mucosa, more than uncoated-NPs and CS-based NPs.
33423571	9	38	theme	gastrointestinal	1069:1084	arg1	tract					1086:1090	the upper gastrointestinal tract	1059:1090	the upper gastrointestinal tract	1059:1090	However, GP and L100 provided an increase in α-mangostin loading, limited the release of α-mangostin in the upper gastrointestinal tract, and enhanced α-mangostin delivery to the colon.
33423571	12	39	theme	TCS-based	1342:1350	arg1	candidate					1377:1385	a promising candidate	1365:1385	a promising candidate for a controlled-release drug delivery system of α-mangostin to the colon	1365:1459	Therefore, the mucoadhesive TCS-based NPs could be a promising candidate for a controlled-release drug delivery system of α-mangostin to the colon.
33423571	12	39	theme	TCS-based	1342:1350	arg1	NPs					1352:1354	the mucoadhesive TCS-based NPs	1325:1354	the mucoadhesive TCS-based NPs	1325:1354	Therefore, the mucoadhesive TCS-based NPs could be a promising candidate for a controlled-release drug delivery system of α-mangostin to the colon.
33423571	8	40	theme	larger	906:911	arg1	NPs					913:915	larger NPs	906:915	larger NPs	906:915	The small size of NPs was found with TCS and larger NPs were observed by GP and L100 process.
33423571	1	41	theme	pH-dependent	261:272	arg1	NPs					297:299	pH-dependent composite mucoadhesive NPs	261:299	pH-dependent composite mucoadhesive NPs	261:299	α-Mangostin-loaded mucoadhesive nanoparticles (NPs) were prepared for colon-targeted drug delivery against colorectal cancer cells using pH-dependent composite mucoadhesive NPs.
33423571	0	42	theme	alpha	70:74	arg1	mangostin					76:84	containing alpha mangostin	59:84	containing alpha mangostin for possible Colon-targeted delivery	59:121	Mucoadhesive chitosan and thiolated chitosan nanoparticles containing alpha mangostin for possible Colon-targeted delivery.
33423571	10	43	theme	CS-based	1250:1257	arg1	NPs					1259:1261	uncoated-NPs and CS-based NPs	1233:1261	uncoated-NPs and CS-based NPs	1233:1261	The TCS-based NPs with GP and L100 exhibited strong mucoadhesion to colon mucosa, more than uncoated-NPs and CS-based NPs.
33423571	7	44	theme	particle	815:822	arg1	size					824:827	particle size	815:827	particle size ranging between 300 and 900 nm	815:858	The results showed that NPs were slightly oblong in shape with particle size ranging between 300 and 900 nm.
33423571	0	45	theme	containing	59:68	arg1	mangostin					76:84	containing alpha mangostin	59:84	containing alpha mangostin for possible Colon-targeted delivery	59:121	Mucoadhesive chitosan and thiolated chitosan nanoparticles containing alpha mangostin for possible Colon-targeted delivery.
33423571	12	46	theme	α-mangostin	1436:1446	arg1	system					1426:1431	a controlled-release drug delivery system	1391:1431	a controlled-release drug delivery system of α-mangostin to the colon	1391:1459	Therefore, the mucoadhesive TCS-based NPs could be a promising candidate for a controlled-release drug delivery system of α-mangostin to the colon.
33423571	9	47	from	delivery	1118:1125	arg1	tract					1086:1090	the upper gastrointestinal tract	1059:1090	the upper gastrointestinal tract	1059:1090	However, GP and L100 provided an increase in α-mangostin loading, limited the release of α-mangostin in the upper gastrointestinal tract, and enhanced α-mangostin delivery to the colon.
33423571	9	48	theme	enhanced	1097:1104	arg1	delivery					1118:1125	enhanced α-mangostin delivery	1097:1125	enhanced α-mangostin delivery to the colon	1097:1138	However, GP and L100 provided an increase in α-mangostin loading, limited the release of α-mangostin in the upper gastrointestinal tract, and enhanced α-mangostin delivery to the colon.
33423571	3	49	theme	particle	470:477	arg1	size					479:482	The particle size	466:482	The particle size	466:482	The particle size, morphologies and characteristics of NPs were observed.
33423571	10	50	theme	strong	1186:1191	arg1	mucoadhesion					1193:1204	strong mucoadhesion	1186:1204	strong mucoadhesion	1186:1204	The TCS-based NPs with GP and L100 exhibited strong mucoadhesion to colon mucosa, more than uncoated-NPs and CS-based NPs.
33423571	1	51	theme	composite	274:282	arg1	NPs					297:299	pH-dependent composite mucoadhesive NPs	261:299	pH-dependent composite mucoadhesive NPs	261:299	α-Mangostin-loaded mucoadhesive nanoparticles (NPs) were prepared for colon-targeted drug delivery against colorectal cancer cells using pH-dependent composite mucoadhesive NPs.
33423571	9	52	theme	α-mangostin	1106:1116	arg1	delivery					1118:1125	enhanced α-mangostin delivery	1097:1125	enhanced α-mangostin delivery to the colon	1097:1138	However, GP and L100 provided an increase in α-mangostin loading, limited the release of α-mangostin in the upper gastrointestinal tract, and enhanced α-mangostin delivery to the colon.
33423571	3	53	theme	NPs	521:523	arg1	characteristics					502:516	characteristics	502:516	characteristics	502:516	The particle size, morphologies and characteristics of NPs were observed.
33423571	3	53	theme	NPs	521:523	arg1	morphologies					485:496	morphologies	485:496	morphologies	485:496	The particle size, morphologies and characteristics of NPs were observed.
33423571	3	53	theme	NPs	521:523	arg1	size					479:482	The particle size	466:482	The particle size	466:482	The particle size, morphologies and characteristics of NPs were observed.
33423571	5	54	dep	In	604:605	arg1	vitro					607:611	vitro	607:611	vitro	607:611	In vitro mucoadhesive properties were examined by the wash-off method.
33423571	1	55	theme	mucoadhesive	284:295	arg1	NPs					297:299	pH-dependent composite mucoadhesive NPs	261:299	pH-dependent composite mucoadhesive NPs	261:299	α-Mangostin-loaded mucoadhesive nanoparticles (NPs) were prepared for colon-targeted drug delivery against colorectal cancer cells using pH-dependent composite mucoadhesive NPs.
33423571	2	56	theme	genipin	385:391	arg1	crosslinking					398:409	by genipin (GP) crosslinking	382:409	by genipin (GP) crosslinking	382:409	Chitosan (CS) and thiolated chitosan (TCS) were used to form the NPs, following by genipin (GP) crosslinking and the surface modification by Eudragit® L100 (L100).
33423571	2	57	used	used	350:353	arg2	TCS					340:342	TCS	340:342	TCS	340:342	Chitosan (CS) and thiolated chitosan (TCS) were used to form the NPs, following by genipin (GP) crosslinking and the surface modification by Eudragit® L100 (L100).
33423571	2	57	used	used	350:353	arg2	CS					312:313	CS	312:313	CS	312:313	Chitosan (CS) and thiolated chitosan (TCS) were used to form the NPs, following by genipin (GP) crosslinking and the surface modification by Eudragit® L100 (L100).
33423571	2	57	used	used	350:353	arg2	Chitosan					302:309	Chitosan	302:309	Chitosan (CS)	302:314	Chitosan (CS) and thiolated chitosan (TCS) were used to form the NPs, following by genipin (GP) crosslinking and the surface modification by Eudragit® L100 (L100).
33423571	2	57	used	used	350:353	arg2	chitosan					330:337	thiolated chitosan	320:337	thiolated chitosan (TCS)	320:343	Chitosan (CS) and thiolated chitosan (TCS) were used to form the NPs, following by genipin (GP) crosslinking and the surface modification by Eudragit® L100 (L100).
33423571	11	58	theme	anti-tumour	1292:1302	arg1	activity					1304:1311	the anti-tumour activity	1288:1311	the anti-tumour activity	1288:1311	Moreover, NPs exhibited the anti-tumour activity.
33423571	12	59	theme	drug	1412:1415	arg1	system					1426:1431	a controlled-release drug delivery system	1391:1431	a controlled-release drug delivery system of α-mangostin to the colon	1391:1459	Therefore, the mucoadhesive TCS-based NPs could be a promising candidate for a controlled-release drug delivery system of α-mangostin to the colon.
33423571	9	60	from	release	1033:1039	arg1	tract					1086:1090	the upper gastrointestinal tract	1059:1090	the upper gastrointestinal tract	1059:1090	However, GP and L100 provided an increase in α-mangostin loading, limited the release of α-mangostin in the upper gastrointestinal tract, and enhanced α-mangostin delivery to the colon.
33423571	6	61	theme	cancer	738:743	arg1	cells					745:749	colorectal cancer cells	727:749	colorectal cancer cells	727:749	In addition, the anti-tumour activity was tested on colorectal cancer cells.
33423571	2	62	theme	by	382:383	arg1	crosslinking					398:409	by genipin (GP) crosslinking	382:409	by genipin (GP) crosslinking	382:409	Chitosan (CS) and thiolated chitosan (TCS) were used to form the NPs, following by genipin (GP) crosslinking and the surface modification by Eudragit® L100 (L100).
33423571	9	63	theme	α-mangostin	1000:1010	arg1	loading					1012:1018	α-mangostin loading	1000:1018	α-mangostin loading	1000:1018	However, GP and L100 provided an increase in α-mangostin loading, limited the release of α-mangostin in the upper gastrointestinal tract, and enhanced α-mangostin delivery to the colon.
31677149	5	0	theme	245.13	1143:1148	arg1	nm					1158:1159	245.13 ± 10.23 nm	1143:1159	245.13 ± 10.23 nm	1143:1159	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	5	0	theme	245.13	1143:1148	arg1	vesicles					1133:1140	spherical vesicles	1123:1140	spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2)	1123:1272	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	2	1	theme	formulation	567:577	arg1	variables					579:587	different formulation variables	557:587	different formulation variables	557:587	A face-centered central composite design was adopted to observe the effects of different formulation variables on TBN-BLS, and artificial neural network (ANN) modeling was employed to optimize TBN-BLS.
31677149	4	2	from	study	1004:1008	arg1	model					1053:1057	a rat model	1047:1057	a rat model	1047:1057	After being subjected to physicochemical characterization, TBN-BLS were enrolled in a histopathological study and pharmacokinetic investigation in a rat model.
31677149	4	3	theme	histopathological	986:1002	arg1	study					1004:1008	histopathological study	986:1008	histopathological study	986:1008	After being subjected to physicochemical characterization, TBN-BLS were enrolled in a histopathological study and pharmacokinetic investigation in a rat model.
31677149	3	4	theme	lipid	795:799	arg1	cholesterol					778:788	cholesterol	778:788	cholesterol	778:788	TBN-BLS were prepared by a thin film hydration method integrating soybean phosphatidylcholine and cholesterol as a lipid phase and sodium deoxycholate (SDC) as a surfactant with or without the coating of chitosan (CTS).
31677149	3	4	theme	lipid	795:799	arg1	phosphatidylcholine					754:772	soybean phosphatidylcholine	746:772	soybean phosphatidylcholine	746:772	TBN-BLS were prepared by a thin film hydration method integrating soybean phosphatidylcholine and cholesterol as a lipid phase and sodium deoxycholate (SDC) as a surfactant with or without the coating of chitosan (CTS).
31677149	3	4	theme	lipid	795:799	arg1	phase					801:805	a lipid phase	793:805	a lipid phase	793:805	TBN-BLS were prepared by a thin film hydration method integrating soybean phosphatidylcholine and cholesterol as a lipid phase and sodium deoxycholate (SDC) as a surfactant with or without the coating of chitosan (CTS).
31677149	5	5	theme	chitosan-coated	1078:1092	arg1	TBN-CTS-BLS					1105:1115	TBN-CTS-BLS	1105:1115	TBN-CTS-BLS	1105:1115	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	5	5	theme	chitosan-coated	1078:1092	arg1	bilosomes					1094:1102	The optimized TBN chitosan-coated bilosomes	1060:1102	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS)	1060:1116	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	5	5	theme	chitosan-coated	1078:1092	arg1	vesicles					1133:1140	spherical vesicles	1123:1140	spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2)	1123:1272	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	0	6	theme	in	176:177	arg1	evaluation					184:193	in vivo evaluation	176:193	in vivo evaluation	176:193	A novel nanogel loaded with chitosan decorated bilosomes for transdermal delivery of terbutaline sulfate: artificial neural network optimization, in vitro characterization and in vivo evaluation.
31677149	0	6	theme	in	176:177	arg1	bilosomes					47:55	bilosomes	47:55	bilosomes for transdermal delivery of terbutaline sulfate: artificial neural network optimization, in vitro characterization and in vivo evaluation	47:193	A novel nanogel loaded with chitosan decorated bilosomes for transdermal delivery of terbutaline sulfate: artificial neural network optimization, in vitro characterization and in vivo evaluation.
31677149	2	7	theme	network	623:629	arg1	modeling					637:644	artificial neural network (ANN) modeling	605:644	artificial neural network (ANN) modeling	605:644	A face-centered central composite design was adopted to observe the effects of different formulation variables on TBN-BLS, and artificial neural network (ANN) modeling was employed to optimize TBN-BLS.
31677149	7	8	theme	TBN	1522:1524	arg1	bioavailability					1503:1517	the bioavailability	1499:1517	the bioavailability of TBN	1499:1524	The pharmacokinetic study revealed that the optimized TBN-CTS-BLS formulation successively enhanced the bioavailability of TBN by about 2.33-fold and increased t1/2 to about 6.21 ± 0.24 h as compared to the oral solution.
31677149	4	9	theme	pharmacokinetic	1014:1028	arg1	investigation					1030:1042	pharmacokinetic investigation	1014:1042	pharmacokinetic investigation	1014:1042	After being subjected to physicochemical characterization, TBN-BLS were enrolled in a histopathological study and pharmacokinetic investigation in a rat model.
31677149	0	10	theme	sulfate	97:103	arg1	delivery					73:80	transdermal delivery	61:80	transdermal delivery of terbutaline sulfate	61:103	A novel nanogel loaded with chitosan decorated bilosomes for transdermal delivery of terbutaline sulfate: artificial neural network optimization, in vitro characterization and in vivo evaluation.
31677149	7	11	theme	optimized	1443:1451	arg1	formulation					1465:1475	the optimized TBN-CTS-BLS formulation	1439:1475	the optimized TBN-CTS-BLS formulation	1439:1475	The pharmacokinetic study revealed that the optimized TBN-CTS-BLS formulation successively enhanced the bioavailability of TBN by about 2.33-fold and increased t1/2 to about 6.21 ± 0.24 h as compared to the oral solution.
31677149	5	12	theme	±	1205:1205	arg1	%					1211:1211	65.25 ± 5.51%	1199:1211	65.25 ± 5.51%	1199:1211	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	5	12	theme	±	1205:1205	arg1	efficiency					1187:1196	adequate entrapment efficiency	1167:1196	adequate entrapment efficiency (65.25 ± 5.51%)	1167:1212	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	2	13	theme	neural	616:621	arg1	ANN					632:634	ANN	632:634	ANN	632:634	A face-centered central composite design was adopted to observe the effects of different formulation variables on TBN-BLS, and artificial neural network (ANN) modeling was employed to optimize TBN-BLS.
31677149	2	13	theme	neural	616:621	arg1	network					623:629	artificial neural network	605:629	artificial neural network (ANN) modeling	605:644	A face-centered central composite design was adopted to observe the effects of different formulation variables on TBN-BLS, and artificial neural network (ANN) modeling was employed to optimize TBN-BLS.
31677149	0	14	theme	terbutaline	85:95	arg1	sulfate					97:103	terbutaline sulfate	85:103	terbutaline sulfate	85:103	A novel nanogel loaded with chitosan decorated bilosomes for transdermal delivery of terbutaline sulfate: artificial neural network optimization, in vitro characterization and in vivo evaluation.
31677149	2	15	theme	artificial	605:614	arg1	ANN					632:634	ANN	632:634	ANN	632:634	A face-centered central composite design was adopted to observe the effects of different formulation variables on TBN-BLS, and artificial neural network (ANN) modeling was employed to optimize TBN-BLS.
31677149	2	15	theme	artificial	605:614	arg1	network					623:629	artificial neural network	605:629	artificial neural network (ANN) modeling	605:644	A face-centered central composite design was adopted to observe the effects of different formulation variables on TBN-BLS, and artificial neural network (ANN) modeling was employed to optimize TBN-BLS.
31677149	7	16	theme	TBN-CTS-BLS	1453:1463	arg1	formulation					1465:1475	the optimized TBN-CTS-BLS formulation	1439:1475	the optimized TBN-CTS-BLS formulation	1439:1475	The pharmacokinetic study revealed that the optimized TBN-CTS-BLS formulation successively enhanced the bioavailability of TBN by about 2.33-fold and increased t1/2 to about 6.21 ± 0.24 h as compared to the oral solution.
31677149	4	17	theme	physicochemical	925:939	arg1	characterization					941:956	physicochemical characterization	925:956	physicochemical characterization	925:956	After being subjected to physicochemical characterization, TBN-BLS were enrolled in a histopathological study and pharmacokinetic investigation in a rat model.
31677149	4	18	from	investigation	1030:1042	arg1	model					1053:1057	a rat model	1047:1057	a rat model	1047:1057	After being subjected to physicochemical characterization, TBN-BLS were enrolled in a histopathological study and pharmacokinetic investigation in a rat model.
31677149	0	19	theme	artificial	106:115	arg1	optimization					132:143	artificial neural network optimization	106:143	artificial neural network optimization	106:143	A novel nanogel loaded with chitosan decorated bilosomes for transdermal delivery of terbutaline sulfate: artificial neural network optimization, in vitro characterization and in vivo evaluation.
31677149	0	19	theme	artificial	106:115	arg1	bilosomes					47:55	bilosomes	47:55	bilosomes for transdermal delivery of terbutaline sulfate: artificial neural network optimization, in vitro characterization and in vivo evaluation	47:193	A novel nanogel loaded with chitosan decorated bilosomes for transdermal delivery of terbutaline sulfate: artificial neural network optimization, in vitro characterization and in vivo evaluation.
31677149	9	20	dep	Abstract	1753:1760	arg1	Graphical					1743:1751	Graphical	1743:1751	Graphical	1743:1751	Graphical Abstract.
31677149	1	21	theme	free	415:418	arg1	TBN					420:422	free TBN	415:422	free TBN	415:422	The objective of the present work was to formulate, optimize, and evaluate transdermal terbutaline sulfate (TBN)-loaded bilosomes (BLS) in gel, compared to conventional oral TBN solution and transdermal gel loaded with free TBN, aiming at evading the hepatic first-pass metabolism.
31677149	5	22	theme	good	1218:1221	arg1	μg/cm2					1266:1271	340.11 ± 22.34 μg/cm2	1251:1271	340.11 ± 22.34 μg/cm2	1251:1271	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	5	22	theme	good	1218:1221	arg1	characteristics					1234:1248	good permeation characteristics	1218:1248	good permeation characteristics (340.11 ± 22.34 μg/cm2)	1218:1272	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	6	23	theme	inflammatory	1334:1345	arg1	signs					1347:1351	no inflammatory signs	1331:1351	no inflammatory signs manifested upon histopathological evaluation	1331:1396	The TBN-CTS-BLS gel formulation was well-tolerated with no inflammatory signs manifested upon histopathological evaluation.
31677149	7	24	theme	pharmacokinetic	1403:1417	arg1	study					1419:1423	The pharmacokinetic study	1399:1423	The pharmacokinetic study	1399:1423	The pharmacokinetic study revealed that the optimized TBN-CTS-BLS formulation successively enhanced the bioavailability of TBN by about 2.33-fold and increased t1/2 to about 6.21 ± 0.24 h as compared to the oral solution.
31677149	5	25	theme	TBN	1074:1076	arg1	TBN-CTS-BLS					1105:1115	TBN-CTS-BLS	1105:1115	TBN-CTS-BLS	1105:1115	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	5	25	theme	TBN	1074:1076	arg1	bilosomes					1094:1102	The optimized TBN chitosan-coated bilosomes	1060:1102	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS)	1060:1116	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	5	25	theme	TBN	1074:1076	arg1	vesicles					1133:1140	spherical vesicles	1123:1140	spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2)	1123:1272	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	5	26	theme	entrapment	1176:1185	arg1	%					1211:1211	65.25 ± 5.51%	1199:1211	65.25 ± 5.51%	1199:1211	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	5	26	theme	entrapment	1176:1185	arg1	efficiency					1187:1196	adequate entrapment efficiency	1167:1196	adequate entrapment efficiency (65.25 ± 5.51%)	1167:1212	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	6	27	theme	TBN-CTS-BLS	1279:1289	arg1	formulation					1295:1305	The TBN-CTS-BLS gel formulation	1275:1305	The TBN-CTS-BLS gel formulation	1275:1305	The TBN-CTS-BLS gel formulation was well-tolerated with no inflammatory signs manifested upon histopathological evaluation.
31677149	0	28	theme	novel	2:6	arg1	nanogel					8:14	A novel nanogel	0:14	A novel nanogel loaded with chitosan	0:35	A novel nanogel loaded with chitosan decorated bilosomes for transdermal delivery of terbutaline sulfate: artificial neural network optimization, in vitro characterization and in vivo evaluation.
31677149	5	29	theme	optimized	1064:1072	arg1	TBN-CTS-BLS					1105:1115	TBN-CTS-BLS	1105:1115	TBN-CTS-BLS	1105:1115	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	5	29	theme	optimized	1064:1072	arg1	bilosomes					1094:1102	The optimized TBN chitosan-coated bilosomes	1060:1102	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS)	1060:1116	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	5	29	theme	optimized	1064:1072	arg1	vesicles					1133:1140	spherical vesicles	1123:1140	spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2)	1123:1272	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	8	30	theme	BLS	1664:1666	arg1	use					1657:1659	the prospect use	1644:1659	the prospect use of BLS as active and safe transdermal carrier for TBN in the treatment of asthma	1644:1740	These findings support the prospect use of BLS as active and safe transdermal carrier for TBN in the treatment of asthma.
31677149	5	31	theme	spherical	1123:1131	arg1	bilosomes					1094:1102	The optimized TBN chitosan-coated bilosomes	1060:1102	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS)	1060:1116	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	5	31	theme	spherical	1123:1131	arg1	nm					1158:1159	245.13 ± 10.23 nm	1143:1159	245.13 ± 10.23 nm	1143:1159	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	5	31	theme	spherical	1123:1131	arg1	vesicles					1133:1140	spherical vesicles	1123:1140	spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2)	1123:1272	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	8	32	theme	safe	1682:1685	arg1	carrier					1699:1705	active and safe transdermal carrier	1671:1705	active and safe transdermal carrier for TBN in the treatment of asthma	1671:1740	These findings support the prospect use of BLS as active and safe transdermal carrier for TBN in the treatment of asthma.
31677149	7	33	theme	oral	1606:1609	arg1	solution					1611:1618	the oral solution	1602:1618	the oral solution	1602:1618	The pharmacokinetic study revealed that the optimized TBN-CTS-BLS formulation successively enhanced the bioavailability of TBN by about 2.33-fold and increased t1/2 to about 6.21 ± 0.24 h as compared to the oral solution.
31677149	2	34	theme	variables	579:587	arg1	effects					546:552	the effects	542:552	the effects of different formulation variables on TBN-BLS	542:598	A face-centered central composite design was adopted to observe the effects of different formulation variables on TBN-BLS, and artificial neural network (ANN) modeling was employed to optimize TBN-BLS.
31677149	5	35	with	vesicles	1133:1140	arg1	μg/cm2					1266:1271	340.11 ± 22.34 μg/cm2	1251:1271	340.11 ± 22.34 μg/cm2	1251:1271	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	5	35	with	vesicles	1133:1140	arg1	characteristics					1234:1248	good permeation characteristics	1218:1248	good permeation characteristics (340.11 ± 22.34 μg/cm2)	1218:1272	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	5	35	with	vesicles	1133:1140	arg1	%					1211:1211	65.25 ± 5.51%	1199:1211	65.25 ± 5.51%	1199:1211	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	5	35	with	vesicles	1133:1140	arg1	efficiency					1187:1196	adequate entrapment efficiency	1167:1196	adequate entrapment efficiency (65.25 ± 5.51%)	1167:1212	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	8	36	theme	transdermal	1687:1697	arg1	carrier					1699:1705	active and safe transdermal carrier	1671:1705	active and safe transdermal carrier for TBN in the treatment of asthma	1671:1740	These findings support the prospect use of BLS as active and safe transdermal carrier for TBN in the treatment of asthma.
31677149	2	37	theme	different	557:565	arg1	variables					579:587	different formulation variables	557:587	different formulation variables	557:587	A face-centered central composite design was adopted to observe the effects of different formulation variables on TBN-BLS, and artificial neural network (ANN) modeling was employed to optimize TBN-BLS.
31677149	2	38	from	effects	546:552	arg1	TBN-BLS					592:598	TBN-BLS	592:598	TBN-BLS	592:598	A face-centered central composite design was adopted to observe the effects of different formulation variables on TBN-BLS, and artificial neural network (ANN) modeling was employed to optimize TBN-BLS.
31677149	0	39	theme	network	124:130	arg1	optimization					132:143	artificial neural network optimization	106:143	artificial neural network optimization	106:143	A novel nanogel loaded with chitosan decorated bilosomes for transdermal delivery of terbutaline sulfate: artificial neural network optimization, in vitro characterization and in vivo evaluation.
31677149	0	39	theme	network	124:130	arg1	bilosomes					47:55	bilosomes	47:55	bilosomes for transdermal delivery of terbutaline sulfate: artificial neural network optimization, in vitro characterization and in vivo evaluation	47:193	A novel nanogel loaded with chitosan decorated bilosomes for transdermal delivery of terbutaline sulfate: artificial neural network optimization, in vitro characterization and in vivo evaluation.
31677149	3	40	theme	chitosan	884:891	arg1	coating					873:879	the coating	869:879	the coating of chitosan (CTS)	869:897	TBN-BLS were prepared by a thin film hydration method integrating soybean phosphatidylcholine and cholesterol as a lipid phase and sodium deoxycholate (SDC) as a surfactant with or without the coating of chitosan (CTS).
31677149	6	41	theme	histopathological	1369:1385	arg1	evaluation					1387:1396	histopathological evaluation	1369:1396	histopathological evaluation	1369:1396	The TBN-CTS-BLS gel formulation was well-tolerated with no inflammatory signs manifested upon histopathological evaluation.
31677149	1	42	theme	transdermal	271:281	arg1	BLS					327:329	BLS	327:329	BLS	327:329	The objective of the present work was to formulate, optimize, and evaluate transdermal terbutaline sulfate (TBN)-loaded bilosomes (BLS) in gel, compared to conventional oral TBN solution and transdermal gel loaded with free TBN, aiming at evading the hepatic first-pass metabolism.
31677149	1	42	theme	transdermal	271:281	arg1	bilosomes					316:324	transdermal terbutaline sulfate (TBN)-loaded bilosomes	271:324	transdermal terbutaline sulfate (TBN)-loaded bilosomes (BLS) in gel	271:337	The objective of the present work was to formulate, optimize, and evaluate transdermal terbutaline sulfate (TBN)-loaded bilosomes (BLS) in gel, compared to conventional oral TBN solution and transdermal gel loaded with free TBN, aiming at evading the hepatic first-pass metabolism.
31677149	0	43	theme	neural	117:122	arg1	optimization					132:143	artificial neural network optimization	106:143	artificial neural network optimization	106:143	A novel nanogel loaded with chitosan decorated bilosomes for transdermal delivery of terbutaline sulfate: artificial neural network optimization, in vitro characterization and in vivo evaluation.
31677149	0	43	theme	neural	117:122	arg1	bilosomes					47:55	bilosomes	47:55	bilosomes for transdermal delivery of terbutaline sulfate: artificial neural network optimization, in vitro characterization and in vivo evaluation	47:193	A novel nanogel loaded with chitosan decorated bilosomes for transdermal delivery of terbutaline sulfate: artificial neural network optimization, in vitro characterization and in vivo evaluation.
31677149	1	44	dep	was	230:232	arg1	compared					340:347	compared	340:347	compared to conventional oral TBN solution and transdermal gel loaded with free TBN, aiming at evading the hepatic first-pass metabolism	340:475	The objective of the present work was to formulate, optimize, and evaluate transdermal terbutaline sulfate (TBN)-loaded bilosomes (BLS) in gel, compared to conventional oral TBN solution and transdermal gel loaded with free TBN, aiming at evading the hepatic first-pass metabolism.
31677149	5	45	theme	±	1258:1258	arg1	μg/cm2					1266:1271	340.11 ± 22.34 μg/cm2	1251:1271	340.11 ± 22.34 μg/cm2	1251:1271	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	5	45	theme	±	1258:1258	arg1	characteristics					1234:1248	good permeation characteristics	1218:1248	good permeation characteristics (340.11 ± 22.34 μg/cm2)	1218:1272	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	5	46	theme	340.11	1251:1256	arg1	μg/cm2					1266:1271	340.11 ± 22.34 μg/cm2	1251:1271	340.11 ± 22.34 μg/cm2	1251:1271	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	5	46	theme	340.11	1251:1256	arg1	characteristics					1234:1248	good permeation characteristics	1218:1248	good permeation characteristics (340.11 ± 22.34 μg/cm2)	1218:1272	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	7	47	theme	6.21	1573:1576	arg1	h					1585:1585	6.21 ± 0.24 h	1573:1585	about 6.21 ± 0.24 h	1567:1585	The pharmacokinetic study revealed that the optimized TBN-CTS-BLS formulation successively enhanced the bioavailability of TBN by about 2.33-fold and increased t1/2 to about 6.21 ± 0.24 h as compared to the oral solution.
31677149	5	48	theme	±	1150:1150	arg1	nm					1158:1159	245.13 ± 10.23 nm	1143:1159	245.13 ± 10.23 nm	1143:1159	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	5	48	theme	±	1150:1150	arg1	vesicles					1133:1140	spherical vesicles	1123:1140	spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2)	1123:1272	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	2	49	theme	composite	502:510	arg1	design					512:517	A face-centered central composite design	478:517	A face-centered central composite design	478:517	A face-centered central composite design was adopted to observe the effects of different formulation variables on TBN-BLS, and artificial neural network (ANN) modeling was employed to optimize TBN-BLS.
31677149	5	50	theme	5.51	1207:1210	arg1	%					1211:1211	65.25 ± 5.51%	1199:1211	65.25 ± 5.51%	1199:1211	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	5	50	theme	5.51	1207:1210	arg1	efficiency					1187:1196	adequate entrapment efficiency	1167:1196	adequate entrapment efficiency (65.25 ± 5.51%)	1167:1212	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	0	51	dep	bilosomes	47:55	arg1	optimization					132:143	artificial neural network optimization	106:143	artificial neural network optimization	106:143	A novel nanogel loaded with chitosan decorated bilosomes for transdermal delivery of terbutaline sulfate: artificial neural network optimization, in vitro characterization and in vivo evaluation.
31677149	0	51	dep	bilosomes	47:55	arg1	evaluation					184:193	in vivo evaluation	176:193	in vivo evaluation	176:193	A novel nanogel loaded with chitosan decorated bilosomes for transdermal delivery of terbutaline sulfate: artificial neural network optimization, in vitro characterization and in vivo evaluation.
31677149	0	51	dep	bilosomes	47:55	arg1	characterization					155:170	in vitro characterization	146:170	in vitro characterization	146:170	A novel nanogel loaded with chitosan decorated bilosomes for transdermal delivery of terbutaline sulfate: artificial neural network optimization, in vitro characterization and in vivo evaluation.
31677149	0	51	dep	bilosomes	47:55	arg1	bilosomes					47:55	bilosomes	47:55	bilosomes for transdermal delivery of terbutaline sulfate: artificial neural network optimization, in vitro characterization and in vivo evaluation	47:193	A novel nanogel loaded with chitosan decorated bilosomes for transdermal delivery of terbutaline sulfate: artificial neural network optimization, in vitro characterization and in vivo evaluation.
31677149	5	52	theme	permeation	1223:1232	arg1	μg/cm2					1266:1271	340.11 ± 22.34 μg/cm2	1251:1271	340.11 ± 22.34 μg/cm2	1251:1271	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	5	52	theme	permeation	1223:1232	arg1	characteristics					1234:1248	good permeation characteristics	1218:1248	good permeation characteristics (340.11 ± 22.34 μg/cm2)	1218:1272	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	3	53	theme	thin	707:710	arg1	film					712:715	a thin film	705:715	a thin film hydration method integrating soybean phosphatidylcholine and cholesterol as a lipid phase and sodium deoxycholate (SDC) as a surfactant with or without the coating of chitosan (CTS)	705:897	TBN-BLS were prepared by a thin film hydration method integrating soybean phosphatidylcholine and cholesterol as a lipid phase and sodium deoxycholate (SDC) as a surfactant with or without the coating of chitosan (CTS).
31677149	2	54	theme	central	494:500	arg1	design					512:517	A face-centered central composite design	478:517	A face-centered central composite design	478:517	A face-centered central composite design was adopted to observe the effects of different formulation variables on TBN-BLS, and artificial neural network (ANN) modeling was employed to optimize TBN-BLS.
31677149	1	55	theme	conventional	352:363	arg1	solution					374:381	conventional oral TBN solution	352:381	conventional oral TBN solution	352:381	The objective of the present work was to formulate, optimize, and evaluate transdermal terbutaline sulfate (TBN)-loaded bilosomes (BLS) in gel, compared to conventional oral TBN solution and transdermal gel loaded with free TBN, aiming at evading the hepatic first-pass metabolism.
31677149	0	56	dep	in	176:177	arg1	vivo					179:182	vivo	179:182	vivo	179:182	A novel nanogel loaded with chitosan decorated bilosomes for transdermal delivery of terbutaline sulfate: artificial neural network optimization, in vitro characterization and in vivo evaluation.
31677149	7	57	theme	±	1578:1578	arg1	h					1585:1585	6.21 ± 0.24 h	1573:1585	about 6.21 ± 0.24 h	1567:1585	The pharmacokinetic study revealed that the optimized TBN-CTS-BLS formulation successively enhanced the bioavailability of TBN by about 2.33-fold and increased t1/2 to about 6.21 ± 0.24 h as compared to the oral solution.
31677149	2	58	theme	face-centered	480:492	arg1	design					512:517	A face-centered central composite design	478:517	A face-centered central composite design	478:517	A face-centered central composite design was adopted to observe the effects of different formulation variables on TBN-BLS, and artificial neural network (ANN) modeling was employed to optimize TBN-BLS.
31677149	1	59	theme	oral	365:368	arg1	solution					374:381	conventional oral TBN solution	352:381	conventional oral TBN solution	352:381	The objective of the present work was to formulate, optimize, and evaluate transdermal terbutaline sulfate (TBN)-loaded bilosomes (BLS) in gel, compared to conventional oral TBN solution and transdermal gel loaded with free TBN, aiming at evading the hepatic first-pass metabolism.
31677149	4	60	dep	study	1004:1008	arg1	a					984:984	a	984:984	a	984:984	After being subjected to physicochemical characterization, TBN-BLS were enrolled in a histopathological study and pharmacokinetic investigation in a rat model.
31677149	1	61	from	bilosomes	316:324	arg1	gel					335:337	gel	335:337	gel	335:337	The objective of the present work was to formulate, optimize, and evaluate transdermal terbutaline sulfate (TBN)-loaded bilosomes (BLS) in gel, compared to conventional oral TBN solution and transdermal gel loaded with free TBN, aiming at evading the hepatic first-pass metabolism.
31677149	3	62	theme	film	712:715	arg1	method					727:732	a thin film hydration method	705:732	a thin film hydration method integrating soybean phosphatidylcholine and cholesterol as a lipid phase and sodium deoxycholate (SDC) as a surfactant with or without the coating of chitosan (CTS)	705:897	TBN-BLS were prepared by a thin film hydration method integrating soybean phosphatidylcholine and cholesterol as a lipid phase and sodium deoxycholate (SDC) as a surfactant with or without the coating of chitosan (CTS).
31677149	0	63	theme	in	146:147	arg1	characterization					155:170	in vitro characterization	146:170	in vitro characterization	146:170	A novel nanogel loaded with chitosan decorated bilosomes for transdermal delivery of terbutaline sulfate: artificial neural network optimization, in vitro characterization and in vivo evaluation.
31677149	0	63	theme	in	146:147	arg1	bilosomes					47:55	bilosomes	47:55	bilosomes for transdermal delivery of terbutaline sulfate: artificial neural network optimization, in vitro characterization and in vivo evaluation	47:193	A novel nanogel loaded with chitosan decorated bilosomes for transdermal delivery of terbutaline sulfate: artificial neural network optimization, in vitro characterization and in vivo evaluation.
31677149	1	64	theme	TBN	370:372	arg1	solution					374:381	conventional oral TBN solution	352:381	conventional oral TBN solution	352:381	The objective of the present work was to formulate, optimize, and evaluate transdermal terbutaline sulfate (TBN)-loaded bilosomes (BLS) in gel, compared to conventional oral TBN solution and transdermal gel loaded with free TBN, aiming at evading the hepatic first-pass metabolism.
31677149	1	65	theme	hepatic	447:453	arg1	metabolism					466:475	the hepatic first-pass metabolism	443:475	the hepatic first-pass metabolism	443:475	The objective of the present work was to formulate, optimize, and evaluate transdermal terbutaline sulfate (TBN)-loaded bilosomes (BLS) in gel, compared to conventional oral TBN solution and transdermal gel loaded with free TBN, aiming at evading the hepatic first-pass metabolism.
31677149	8	66	theme	active	1671:1676	arg1	carrier					1699:1705	active and safe transdermal carrier	1671:1705	active and safe transdermal carrier for TBN in the treatment of asthma	1671:1740	These findings support the prospect use of BLS as active and safe transdermal carrier for TBN in the treatment of asthma.
31677149	3	67	theme	hydration	717:725	arg1	method					727:732	a thin film hydration method	705:732	a thin film hydration method integrating soybean phosphatidylcholine and cholesterol as a lipid phase and sodium deoxycholate (SDC) as a surfactant with or without the coating of chitosan (CTS)	705:897	TBN-BLS were prepared by a thin film hydration method integrating soybean phosphatidylcholine and cholesterol as a lipid phase and sodium deoxycholate (SDC) as a surfactant with or without the coating of chitosan (CTS).
31677149	1	68	theme	first-pass	455:464	arg1	metabolism					466:475	the hepatic first-pass metabolism	443:475	the hepatic first-pass metabolism	443:475	The objective of the present work was to formulate, optimize, and evaluate transdermal terbutaline sulfate (TBN)-loaded bilosomes (BLS) in gel, compared to conventional oral TBN solution and transdermal gel loaded with free TBN, aiming at evading the hepatic first-pass metabolism.
31677149	1	69	theme	present	217:223	arg1	work					225:228	the present work	213:228	the present work	213:228	The objective of the present work was to formulate, optimize, and evaluate transdermal terbutaline sulfate (TBN)-loaded bilosomes (BLS) in gel, compared to conventional oral TBN solution and transdermal gel loaded with free TBN, aiming at evading the hepatic first-pass metabolism.
31677149	8	70	from	carrier	1699:1705	arg1	treatment					1722:1730	the treatment	1718:1730	the treatment of asthma	1718:1740	These findings support the prospect use of BLS as active and safe transdermal carrier for TBN in the treatment of asthma.
31677149	8	71	theme	asthma	1735:1740	arg1	treatment					1722:1730	the treatment	1718:1730	the treatment of asthma	1718:1740	These findings support the prospect use of BLS as active and safe transdermal carrier for TBN in the treatment of asthma.
31677149	0	72	dep	in	146:147	arg1	vitro					149:153	vitro	149:153	vitro	149:153	A novel nanogel loaded with chitosan decorated bilosomes for transdermal delivery of terbutaline sulfate: artificial neural network optimization, in vitro characterization and in vivo evaluation.
31677149	4	73	theme	rat	1049:1051	arg1	model					1053:1057	a rat model	1047:1057	a rat model	1047:1057	After being subjected to physicochemical characterization, TBN-BLS were enrolled in a histopathological study and pharmacokinetic investigation in a rat model.
31677149	1	74	theme	work	225:228	arg1	objective					200:208	The objective	196:208	The objective of the present work	196:228	The objective of the present work was to formulate, optimize, and evaluate transdermal terbutaline sulfate (TBN)-loaded bilosomes (BLS) in gel, compared to conventional oral TBN solution and transdermal gel loaded with free TBN, aiming at evading the hepatic first-pass metabolism.
31677149	0	75	theme	transdermal	61:71	arg1	delivery					73:80	transdermal delivery	61:80	transdermal delivery of terbutaline sulfate	61:103	A novel nanogel loaded with chitosan decorated bilosomes for transdermal delivery of terbutaline sulfate: artificial neural network optimization, in vitro characterization and in vivo evaluation.
31677149	1	76	theme	transdermal	387:397	arg1	gel					399:401	transdermal gel	387:401	transdermal gel	387:401	The objective of the present work was to formulate, optimize, and evaluate transdermal terbutaline sulfate (TBN)-loaded bilosomes (BLS) in gel, compared to conventional oral TBN solution and transdermal gel loaded with free TBN, aiming at evading the hepatic first-pass metabolism.
31677149	3	77	theme	sodium	811:816	arg1	deoxycholate					818:829	sodium deoxycholate	811:829	sodium deoxycholate (SDC)	811:835	TBN-BLS were prepared by a thin film hydration method integrating soybean phosphatidylcholine and cholesterol as a lipid phase and sodium deoxycholate (SDC) as a surfactant with or without the coating of chitosan (CTS).
31677149	3	77	theme	sodium	811:816	arg1	SDC					832:834	SDC	832:834	SDC	832:834	TBN-BLS were prepared by a thin film hydration method integrating soybean phosphatidylcholine and cholesterol as a lipid phase and sodium deoxycholate (SDC) as a surfactant with or without the coating of chitosan (CTS).
31677149	3	78	theme	soybean	746:752	arg1	surfactant					842:851	a surfactant	840:851	a surfactant with or without the coating of chitosan (CTS)	840:897	TBN-BLS were prepared by a thin film hydration method integrating soybean phosphatidylcholine and cholesterol as a lipid phase and sodium deoxycholate (SDC) as a surfactant with or without the coating of chitosan (CTS).
31677149	3	78	theme	soybean	746:752	arg1	cholesterol					778:788	cholesterol	778:788	cholesterol	778:788	TBN-BLS were prepared by a thin film hydration method integrating soybean phosphatidylcholine and cholesterol as a lipid phase and sodium deoxycholate (SDC) as a surfactant with or without the coating of chitosan (CTS).
31677149	3	78	theme	soybean	746:752	arg1	phosphatidylcholine					754:772	soybean phosphatidylcholine	746:772	soybean phosphatidylcholine	746:772	TBN-BLS were prepared by a thin film hydration method integrating soybean phosphatidylcholine and cholesterol as a lipid phase and sodium deoxycholate (SDC) as a surfactant with or without the coating of chitosan (CTS).
31677149	3	78	theme	soybean	746:752	arg1	phase					801:805	a lipid phase	793:805	a lipid phase	793:805	TBN-BLS were prepared by a thin film hydration method integrating soybean phosphatidylcholine and cholesterol as a lipid phase and sodium deoxycholate (SDC) as a surfactant with or without the coating of chitosan (CTS).
31677149	1	79	theme	-loaded	308:314	arg1	BLS					327:329	BLS	327:329	BLS	327:329	The objective of the present work was to formulate, optimize, and evaluate transdermal terbutaline sulfate (TBN)-loaded bilosomes (BLS) in gel, compared to conventional oral TBN solution and transdermal gel loaded with free TBN, aiming at evading the hepatic first-pass metabolism.
31677149	1	79	theme	-loaded	308:314	arg1	bilosomes					316:324	transdermal terbutaline sulfate (TBN)-loaded bilosomes	271:324	transdermal terbutaline sulfate (TBN)-loaded bilosomes (BLS) in gel	271:337	The objective of the present work was to formulate, optimize, and evaluate transdermal terbutaline sulfate (TBN)-loaded bilosomes (BLS) in gel, compared to conventional oral TBN solution and transdermal gel loaded with free TBN, aiming at evading the hepatic first-pass metabolism.
31677149	5	80	theme	adequate	1167:1174	arg1	%					1211:1211	65.25 ± 5.51%	1199:1211	65.25 ± 5.51%	1199:1211	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	5	80	theme	adequate	1167:1174	arg1	efficiency					1187:1196	adequate entrapment efficiency	1167:1196	adequate entrapment efficiency (65.25 ± 5.51%)	1167:1212	The optimized TBN chitosan-coated bilosomes (TBN-CTS-BLS) were spherical vesicles (245.13 ± 10.23 nm) with adequate entrapment efficiency (65.25 ± 5.51%) and good permeation characteristics (340.11 ± 22.34 μg/cm2).
31677149	8	81	theme	prospect	1648:1655	arg1	use					1657:1659	the prospect use	1644:1659	the prospect use of BLS as active and safe transdermal carrier for TBN in the treatment of asthma	1644:1740	These findings support the prospect use of BLS as active and safe transdermal carrier for TBN in the treatment of asthma.
31677149	6	82	theme	gel	1291:1293	arg1	formulation					1295:1305	The TBN-CTS-BLS gel formulation	1275:1305	The TBN-CTS-BLS gel formulation	1275:1305	The TBN-CTS-BLS gel formulation was well-tolerated with no inflammatory signs manifested upon histopathological evaluation.
33059004	0	0	theme	transdermal	88:98	arg1	delivery					105:112	transdermal drug delivery	88:112	transdermal drug delivery	88:112	Study on the fabrication and characterization of tip-loaded dissolving microneedles for transdermal drug delivery.
33059004	7	1	theme	smooth	1153:1158	arg1	skin					1160:1163	smooth skin	1153:1163	smooth skin	1153:1163	Nanoindentation testing showed that the tips of all three kinds of dissolving microneedles can bear the maximum loading of 50 mN with no damages, indicated sufficient mechanical strength for smooth skin puncturing as the minimum pressure required was 10 mN only.
33059004	7	2	theme	kinds	1020:1024	arg1	tips					1002:1005	the tips	998:1005	the tips of all three kinds of dissolving microneedles	998:1051	Nanoindentation testing showed that the tips of all three kinds of dissolving microneedles can bear the maximum loading of 50 mN with no damages, indicated sufficient mechanical strength for smooth skin puncturing as the minimum pressure required was 10 mN only.
33059004	2	3	theme	layered	360:366	arg1	microneedles					368:379	layered microneedles	360:379	layered microneedles	360:379	The tip-loaded dissolving microneedles, also named layered microneedles, was consisted of two layers.
33059004	6	4	theme	fabricating	818:828	arg1	conditions					830:839	fabricating conditions	818:839	fabricating conditions	818:839	The material formulation and fabricating conditions of the tip-loaded dissolving microneedles and their transdermal insulin delivery efficiency were systematically studied.
33059004	1	5	theme	drug	160:163	arg1	rate					152:155	the utilization rate	136:155	the utilization rate of drug carried by microneedles	136:187	In order to increase the utilization rate of drug carried by microneedles and reduce waste, a two-step casting method was proposed to fabricate tip-loaded dissolving microneedles in this paper.
33059004	4	6	theme	hyaluronic	579:588	arg1	polyvinylpyrrolidone					554:573	polyvinylpyrrolidone	554:573	polyvinylpyrrolidone	554:573	Polyvinyl alcohol, polyvinylpyrrolidone and hyaluronic acid were used as the base materials to fabricate the dissolving layers of the microneedle patches.
33059004	4	6	theme	hyaluronic	579:588	arg1	acid					590:593	hyaluronic acid	579:593	hyaluronic acid	579:593	Polyvinyl alcohol, polyvinylpyrrolidone and hyaluronic acid were used as the base materials to fabricate the dissolving layers of the microneedle patches.
33059004	4	6	theme	hyaluronic	579:588	arg1	alcohol					545:551	Polyvinyl alcohol	535:551	Polyvinyl alcohol	535:551	Polyvinyl alcohol, polyvinylpyrrolidone and hyaluronic acid were used as the base materials to fabricate the dissolving layers of the microneedle patches.
33059004	4	6	theme	hyaluronic	579:588	arg1	materials					617:625	the base materials	608:625	the base materials	608:625	Polyvinyl alcohol, polyvinylpyrrolidone and hyaluronic acid were used as the base materials to fabricate the dissolving layers of the microneedle patches.
33059004	5	7	theme	model	720:724	arg1	B					700:700	Rhodamine B	690:700	Rhodamine B	690:700	Rhodamine B was chosen as the model drug to show the layered structure of tip-loaded microneedles.
33059004	5	7	theme	model	720:724	arg1	drug					726:729	the model drug	716:729	the model drug to show the layered structure of tip-loaded microneedles	716:786	Rhodamine B was chosen as the model drug to show the layered structure of tip-loaded microneedles.
33059004	0	8	from	Study	0:4	arg1	characterization					29:44	characterization	29:44	characterization	29:44	Study on the fabrication and characterization of tip-loaded dissolving microneedles for transdermal drug delivery.
33059004	0	8	from	Study	0:4	arg1	fabrication					13:23	fabrication	13:23	fabrication	13:23	Study on the fabrication and characterization of tip-loaded dissolving microneedles for transdermal drug delivery.
33059004	0	9	theme	drug	100:103	arg1	delivery					105:112	transdermal drug delivery	88:112	transdermal drug delivery	88:112	Study on the fabrication and characterization of tip-loaded dissolving microneedles for transdermal drug delivery.
33059004	6	10	theme	efficiency	922:931	arg1	conditions					830:839	fabricating conditions	818:839	fabricating conditions	818:839	The material formulation and fabricating conditions of the tip-loaded dissolving microneedles and their transdermal insulin delivery efficiency were systematically studied.
33059004	6	10	theme	efficiency	922:931	arg1	material					793:800	The material formulation and fabricating conditions	789:839	material	793:800	The material formulation and fabricating conditions of the tip-loaded dissolving microneedles and their transdermal insulin delivery efficiency were systematically studied.
33059004	1	11	theme	tip-loaded	259:268	arg1	microneedles					281:292	tip-loaded dissolving microneedles	259:292	tip-loaded dissolving microneedles	259:292	In order to increase the utilization rate of drug carried by microneedles and reduce waste, a two-step casting method was proposed to fabricate tip-loaded dissolving microneedles in this paper.
33059004	6	12	theme	delivery	913:920	arg1	efficiency					922:931	their transdermal insulin delivery efficiency	887:931	their transdermal insulin delivery efficiency	887:931	The material formulation and fabricating conditions of the tip-loaded dissolving microneedles and their transdermal insulin delivery efficiency were systematically studied.
33059004	7	13	theme	sufficient	1118:1127	arg1	strength					1140:1147	sufficient mechanical strength	1118:1147	sufficient mechanical strength for smooth skin	1118:1163	Nanoindentation testing showed that the tips of all three kinds of dissolving microneedles can bear the maximum loading of 50 mN with no damages, indicated sufficient mechanical strength for smooth skin puncturing as the minimum pressure required was 10 mN only.
33059004	3	14	theme	dissolving	514:523	arg1	material					525:532	pure dissolving material	509:532	pure dissolving material	509:532	The tip layer of the microneedles carried model drug, while the backing layer was fabricated with pure dissolving material.
33059004	7	15	contain	bear	1057:1060	arg1	tips					1002:1005	the tips	998:1005	the tips of all three kinds of dissolving microneedles	998:1051	Nanoindentation testing showed that the tips of all three kinds of dissolving microneedles can bear the maximum loading of 50 mN with no damages, indicated sufficient mechanical strength for smooth skin puncturing as the minimum pressure required was 10 mN only.
33059004	7	15	contain	bear	1057:1060	arg2	loading					1074:1080	the maximum loading	1062:1080	the maximum loading of 50 mN with no damages	1062:1105	Nanoindentation testing showed that the tips of all three kinds of dissolving microneedles can bear the maximum loading of 50 mN with no damages, indicated sufficient mechanical strength for smooth skin puncturing as the minimum pressure required was 10 mN only.
33059004	6	16	theme	insulin	905:911	arg1	efficiency					922:931	their transdermal insulin delivery efficiency	887:931	their transdermal insulin delivery efficiency	887:931	The material formulation and fabricating conditions of the tip-loaded dissolving microneedles and their transdermal insulin delivery efficiency were systematically studied.
33059004	7	17	theme	mechanical	1129:1138	arg1	strength					1140:1147	sufficient mechanical strength	1118:1147	sufficient mechanical strength for smooth skin	1118:1163	Nanoindentation testing showed that the tips of all three kinds of dissolving microneedles can bear the maximum loading of 50 mN with no damages, indicated sufficient mechanical strength for smooth skin puncturing as the minimum pressure required was 10 mN only.
33059004	6	18	theme	dissolving	859:868	arg1	microneedles					870:881	the tip-loaded dissolving microneedles	844:881	the tip-loaded dissolving microneedles	844:881	The material formulation and fabricating conditions of the tip-loaded dissolving microneedles and their transdermal insulin delivery efficiency were systematically studied.
33059004	3	19	theme	microneedles	432:443	arg1	layer					419:423	The tip layer	411:423	The tip layer of the microneedles	411:443	The tip layer of the microneedles carried model drug, while the backing layer was fabricated with pure dissolving material.
33059004	6	20	theme	transdermal	893:903	arg1	efficiency					922:931	their transdermal insulin delivery efficiency	887:931	their transdermal insulin delivery efficiency	887:931	The material formulation and fabricating conditions of the tip-loaded dissolving microneedles and their transdermal insulin delivery efficiency were systematically studied.
33059004	2	21	theme	tip-loaded	313:322	arg1	microneedles					335:346	The tip-loaded dissolving microneedles	309:346	The tip-loaded dissolving microneedles	309:346	The tip-loaded dissolving microneedles, also named layered microneedles, was consisted of two layers.
33059004	4	22	theme	microneedle	669:679	arg1	patches					681:687	the microneedle patches	665:687	the microneedle patches	665:687	Polyvinyl alcohol, polyvinylpyrrolidone and hyaluronic acid were used as the base materials to fabricate the dissolving layers of the microneedle patches.
33059004	1	23	theme	dissolving	270:279	arg1	microneedles					281:292	tip-loaded dissolving microneedles	259:292	tip-loaded dissolving microneedles	259:292	In order to increase the utilization rate of drug carried by microneedles and reduce waste, a two-step casting method was proposed to fabricate tip-loaded dissolving microneedles in this paper.
33059004	7	24	theme	Nanoindentation	962:976	arg1	testing					978:984	Nanoindentation testing	962:984	Nanoindentation testing	962:984	Nanoindentation testing showed that the tips of all three kinds of dissolving microneedles can bear the maximum loading of 50 mN with no damages, indicated sufficient mechanical strength for smooth skin puncturing as the minimum pressure required was 10 mN only.
33059004	8	25	theme	%	1388:1388	arg1	enhancement					1390:1400	a 30% enhancement	1384:1400	a 30% enhancement of drug delivery efficiency	1384:1428	Moreover, our fabricated tip-loaded dissolving microneedles can greatly reduce the drug waste cause by unused backing layer in normal microneedles and realize a 30% enhancement of drug delivery efficiency after puncture treatment.
33059004	8	26	theme	unused	1328:1333	arg1	layer					1343:1347	unused backing layer	1328:1347	unused backing layer in normal microneedles	1328:1370	Moreover, our fabricated tip-loaded dissolving microneedles can greatly reduce the drug waste cause by unused backing layer in normal microneedles and realize a 30% enhancement of drug delivery efficiency after puncture treatment.
33059004	8	27	from	layer	1343:1347	arg1	microneedles					1359:1370	normal microneedles	1352:1370	normal microneedles	1352:1370	Moreover, our fabricated tip-loaded dissolving microneedles can greatly reduce the drug waste cause by unused backing layer in normal microneedles and realize a 30% enhancement of drug delivery efficiency after puncture treatment.
33059004	8	28	theme	drug	1405:1408	arg1	efficiency					1419:1428	drug delivery efficiency	1405:1428	drug delivery efficiency	1405:1428	Moreover, our fabricated tip-loaded dissolving microneedles can greatly reduce the drug waste cause by unused backing layer in normal microneedles and realize a 30% enhancement of drug delivery efficiency after puncture treatment.
33059004	6	29	dep	material	793:800	arg1	formulation					802:812	formulation	802:812	formulation	802:812	The material formulation and fabricating conditions of the tip-loaded dissolving microneedles and their transdermal insulin delivery efficiency were systematically studied.
33059004	8	30	theme	30	1386:1387	arg1	%					1388:1388	%	1388:1388	%	1388:1388	Moreover, our fabricated tip-loaded dissolving microneedles can greatly reduce the drug waste cause by unused backing layer in normal microneedles and realize a 30% enhancement of drug delivery efficiency after puncture treatment.
33059004	8	31	theme	delivery	1410:1417	arg1	efficiency					1419:1428	drug delivery efficiency	1405:1428	drug delivery efficiency	1405:1428	Moreover, our fabricated tip-loaded dissolving microneedles can greatly reduce the drug waste cause by unused backing layer in normal microneedles and realize a 30% enhancement of drug delivery efficiency after puncture treatment.
33059004	8	32	theme	normal	1352:1357	arg1	microneedles					1359:1370	normal microneedles	1352:1370	normal microneedles	1352:1370	Moreover, our fabricated tip-loaded dissolving microneedles can greatly reduce the drug waste cause by unused backing layer in normal microneedles and realize a 30% enhancement of drug delivery efficiency after puncture treatment.
33059004	7	33	theme	minimum	1183:1189	arg1	mN					1216:1217	10 mN only	1213:1222	10 mN only	1213:1222	Nanoindentation testing showed that the tips of all three kinds of dissolving microneedles can bear the maximum loading of 50 mN with no damages, indicated sufficient mechanical strength for smooth skin puncturing as the minimum pressure required was 10 mN only.
33059004	7	33	theme	minimum	1183:1189	arg1	pressure					1191:1198	the minimum pressure	1179:1198	the minimum pressure required	1179:1207	Nanoindentation testing showed that the tips of all three kinds of dissolving microneedles can bear the maximum loading of 50 mN with no damages, indicated sufficient mechanical strength for smooth skin puncturing as the minimum pressure required was 10 mN only.
33059004	5	34	theme	tip-loaded	764:773	arg1	microneedles					775:786	tip-loaded microneedles	764:786	tip-loaded microneedles	764:786	Rhodamine B was chosen as the model drug to show the layered structure of tip-loaded microneedles.
33059004	7	35	theme	mN	1088:1089	arg1	loading					1074:1080	the maximum loading	1062:1080	the maximum loading of 50 mN with no damages	1062:1105	Nanoindentation testing showed that the tips of all three kinds of dissolving microneedles can bear the maximum loading of 50 mN with no damages, indicated sufficient mechanical strength for smooth skin puncturing as the minimum pressure required was 10 mN only.
33059004	3	36	contain	carried	445:451	arg1	layer					419:423	The tip layer	411:423	The tip layer of the microneedles	411:443	The tip layer of the microneedles carried model drug, while the backing layer was fabricated with pure dissolving material.
33059004	3	36	contain	carried	445:451	arg2	drug					459:462	model drug	453:462	model drug	453:462	The tip layer of the microneedles carried model drug, while the backing layer was fabricated with pure dissolving material.
33059004	8	37	theme	efficiency	1419:1428	arg1	enhancement					1390:1400	a 30% enhancement	1384:1400	a 30% enhancement of drug delivery efficiency	1384:1428	Moreover, our fabricated tip-loaded dissolving microneedles can greatly reduce the drug waste cause by unused backing layer in normal microneedles and realize a 30% enhancement of drug delivery efficiency after puncture treatment.
33059004	4	38	theme	dissolving	644:653	arg1	layers					655:660	the dissolving layers	640:660	the dissolving layers of the microneedle patches	640:687	Polyvinyl alcohol, polyvinylpyrrolidone and hyaluronic acid were used as the base materials to fabricate the dissolving layers of the microneedle patches.
33059004	3	39	theme	tip	415:417	arg1	layer					419:423	The tip layer	411:423	The tip layer of the microneedles	411:443	The tip layer of the microneedles carried model drug, while the backing layer was fabricated with pure dissolving material.
33059004	5	40	theme	microneedles	775:786	arg1	structure					751:759	the layered structure	739:759	the layered structure of tip-loaded microneedles	739:786	Rhodamine B was chosen as the model drug to show the layered structure of tip-loaded microneedles.
33059004	6	41	theme	microneedles	870:881	arg1	conditions					830:839	fabricating conditions	818:839	fabricating conditions	818:839	The material formulation and fabricating conditions of the tip-loaded dissolving microneedles and their transdermal insulin delivery efficiency were systematically studied.
33059004	6	41	theme	microneedles	870:881	arg1	material					793:800	The material formulation and fabricating conditions	789:839	material	793:800	The material formulation and fabricating conditions of the tip-loaded dissolving microneedles and their transdermal insulin delivery efficiency were systematically studied.
33059004	7	42	theme	only	1219:1222	arg1	mN					1216:1217	10 mN only	1213:1222	10 mN only	1213:1222	Nanoindentation testing showed that the tips of all three kinds of dissolving microneedles can bear the maximum loading of 50 mN with no damages, indicated sufficient mechanical strength for smooth skin puncturing as the minimum pressure required was 10 mN only.
33059004	7	42	theme	only	1219:1222	arg1	pressure					1191:1198	the minimum pressure	1179:1198	the minimum pressure required	1179:1207	Nanoindentation testing showed that the tips of all three kinds of dissolving microneedles can bear the maximum loading of 50 mN with no damages, indicated sufficient mechanical strength for smooth skin puncturing as the minimum pressure required was 10 mN only.
33059004	3	43	theme	model	453:457	arg1	drug					459:462	model drug	453:462	model drug	453:462	The tip layer of the microneedles carried model drug, while the backing layer was fabricated with pure dissolving material.
33059004	0	44	theme	tip-loaded	49:58	arg1	dissolving					60:69	tip-loaded dissolving	49:69	tip-loaded dissolving	49:69	Study on the fabrication and characterization of tip-loaded dissolving microneedles for transdermal drug delivery.
33059004	5	45	theme	layered	743:749	arg1	structure					751:759	the layered structure	739:759	the layered structure of tip-loaded microneedles	739:786	Rhodamine B was chosen as the model drug to show the layered structure of tip-loaded microneedles.
33059004	8	46	theme	puncture	1436:1443	arg1	treatment					1445:1453	puncture treatment	1436:1453	puncture treatment	1436:1453	Moreover, our fabricated tip-loaded dissolving microneedles can greatly reduce the drug waste cause by unused backing layer in normal microneedles and realize a 30% enhancement of drug delivery efficiency after puncture treatment.
33059004	7	47	theme	maximum	1066:1072	arg1	loading					1074:1080	the maximum loading	1062:1080	the maximum loading of 50 mN with no damages	1062:1105	Nanoindentation testing showed that the tips of all three kinds of dissolving microneedles can bear the maximum loading of 50 mN with no damages, indicated sufficient mechanical strength for smooth skin puncturing as the minimum pressure required was 10 mN only.
33059004	3	48	theme	backing	475:481	arg1	layer					483:487	the backing layer	471:487	the backing layer	471:487	The tip layer of the microneedles carried model drug, while the backing layer was fabricated with pure dissolving material.
33059004	8	49	theme	fabricated	1239:1248	arg1	microneedles					1272:1283	our fabricated tip-loaded dissolving microneedles	1235:1283	our fabricated tip-loaded dissolving microneedles	1235:1283	Moreover, our fabricated tip-loaded dissolving microneedles can greatly reduce the drug waste cause by unused backing layer in normal microneedles and realize a 30% enhancement of drug delivery efficiency after puncture treatment.
33059004	4	50	theme	Polyvinyl	535:543	arg1	polyvinylpyrrolidone					554:573	polyvinylpyrrolidone	554:573	polyvinylpyrrolidone	554:573	Polyvinyl alcohol, polyvinylpyrrolidone and hyaluronic acid were used as the base materials to fabricate the dissolving layers of the microneedle patches.
33059004	4	50	theme	Polyvinyl	535:543	arg1	acid					590:593	hyaluronic acid	579:593	hyaluronic acid	579:593	Polyvinyl alcohol, polyvinylpyrrolidone and hyaluronic acid were used as the base materials to fabricate the dissolving layers of the microneedle patches.
33059004	4	50	theme	Polyvinyl	535:543	arg1	alcohol					545:551	Polyvinyl alcohol	535:551	Polyvinyl alcohol	535:551	Polyvinyl alcohol, polyvinylpyrrolidone and hyaluronic acid were used as the base materials to fabricate the dissolving layers of the microneedle patches.
33059004	4	50	theme	Polyvinyl	535:543	arg1	materials					617:625	the base materials	608:625	the base materials	608:625	Polyvinyl alcohol, polyvinylpyrrolidone and hyaluronic acid were used as the base materials to fabricate the dissolving layers of the microneedle patches.
33059004	4	51	used	used	600:603	arg2	materials					617:625	the base materials	608:625	the base materials	608:625	Polyvinyl alcohol, polyvinylpyrrolidone and hyaluronic acid were used as the base materials to fabricate the dissolving layers of the microneedle patches.
33059004	4	51	used	used	600:603	arg2	polyvinylpyrrolidone					554:573	polyvinylpyrrolidone	554:573	polyvinylpyrrolidone	554:573	Polyvinyl alcohol, polyvinylpyrrolidone and hyaluronic acid were used as the base materials to fabricate the dissolving layers of the microneedle patches.
33059004	4	51	used	used	600:603	arg2	alcohol					545:551	Polyvinyl alcohol	535:551	Polyvinyl alcohol	535:551	Polyvinyl alcohol, polyvinylpyrrolidone and hyaluronic acid were used as the base materials to fabricate the dissolving layers of the microneedle patches.
33059004	4	51	used	used	600:603	arg2	acid					590:593	hyaluronic acid	579:593	hyaluronic acid	579:593	Polyvinyl alcohol, polyvinylpyrrolidone and hyaluronic acid were used as the base materials to fabricate the dissolving layers of the microneedle patches.
33059004	6	52	theme	tip-loaded	848:857	arg1	microneedles					870:881	the tip-loaded dissolving microneedles	844:881	the tip-loaded dissolving microneedles	844:881	The material formulation and fabricating conditions of the tip-loaded dissolving microneedles and their transdermal insulin delivery efficiency were systematically studied.
33059004	7	53	theme	microneedles	1040:1051	arg1	kinds					1020:1024	all three kinds	1010:1024	all three kinds of dissolving microneedles	1010:1051	Nanoindentation testing showed that the tips of all three kinds of dissolving microneedles can bear the maximum loading of 50 mN with no damages, indicated sufficient mechanical strength for smooth skin puncturing as the minimum pressure required was 10 mN only.
33059004	5	54	theme	Rhodamine	690:698	arg1	B					700:700	Rhodamine B	690:700	Rhodamine B	690:700	Rhodamine B was chosen as the model drug to show the layered structure of tip-loaded microneedles.
33059004	5	54	theme	Rhodamine	690:698	arg1	drug					726:729	the model drug	716:729	the model drug to show the layered structure of tip-loaded microneedles	716:786	Rhodamine B was chosen as the model drug to show the layered structure of tip-loaded microneedles.
33059004	0	55	dep	fabrication	13:23	arg1	the					9:11	the	9:11	the	9:11	Study on the fabrication and characterization of tip-loaded dissolving microneedles for transdermal drug delivery.
33059004	4	56	theme	patches	681:687	arg1	layers					655:660	the dissolving layers	640:660	the dissolving layers of the microneedle patches	640:687	Polyvinyl alcohol, polyvinylpyrrolidone and hyaluronic acid were used as the base materials to fabricate the dissolving layers of the microneedle patches.
33059004	8	57	theme	backing	1335:1341	arg1	layer					1343:1347	unused backing layer	1328:1347	unused backing layer in normal microneedles	1328:1370	Moreover, our fabricated tip-loaded dissolving microneedles can greatly reduce the drug waste cause by unused backing layer in normal microneedles and realize a 30% enhancement of drug delivery efficiency after puncture treatment.
33059004	1	58	theme	two-step	209:216	arg1	method					226:231	a two-step casting method	207:231	a two-step casting method	207:231	In order to increase the utilization rate of drug carried by microneedles and reduce waste, a two-step casting method was proposed to fabricate tip-loaded dissolving microneedles in this paper.
33059004	2	59	theme	dissolving	324:333	arg1	microneedles					335:346	The tip-loaded dissolving microneedles	309:346	The tip-loaded dissolving microneedles	309:346	The tip-loaded dissolving microneedles, also named layered microneedles, was consisted of two layers.
33059004	8	60	theme	tip-loaded	1250:1259	arg1	microneedles					1272:1283	our fabricated tip-loaded dissolving microneedles	1235:1283	our fabricated tip-loaded dissolving microneedles	1235:1283	Moreover, our fabricated tip-loaded dissolving microneedles can greatly reduce the drug waste cause by unused backing layer in normal microneedles and realize a 30% enhancement of drug delivery efficiency after puncture treatment.
33059004	1	61	theme	casting	218:224	arg1	method					226:231	a two-step casting method	207:231	a two-step casting method	207:231	In order to increase the utilization rate of drug carried by microneedles and reduce waste, a two-step casting method was proposed to fabricate tip-loaded dissolving microneedles in this paper.
33059004	8	62	theme	drug	1308:1311	arg1	cause					1319:1323	the drug waste cause	1304:1323	the drug waste cause	1304:1323	Moreover, our fabricated tip-loaded dissolving microneedles can greatly reduce the drug waste cause by unused backing layer in normal microneedles and realize a 30% enhancement of drug delivery efficiency after puncture treatment.
33059004	7	63	theme	dissolving	1029:1038	arg1	microneedles					1040:1051	dissolving microneedles	1029:1051	dissolving microneedles	1029:1051	Nanoindentation testing showed that the tips of all three kinds of dissolving microneedles can bear the maximum loading of 50 mN with no damages, indicated sufficient mechanical strength for smooth skin puncturing as the minimum pressure required was 10 mN only.
33059004	1	64	theme	utilization	140:150	arg1	rate					152:155	the utilization rate	136:155	the utilization rate of drug carried by microneedles	136:187	In order to increase the utilization rate of drug carried by microneedles and reduce waste, a two-step casting method was proposed to fabricate tip-loaded dissolving microneedles in this paper.
33059004	7	65	with	loading	1074:1080	arg1	damages					1099:1105	no damages	1096:1105	no damages	1096:1105	Nanoindentation testing showed that the tips of all three kinds of dissolving microneedles can bear the maximum loading of 50 mN with no damages, indicated sufficient mechanical strength for smooth skin puncturing as the minimum pressure required was 10 mN only.
33059004	0	66	theme	dissolving	60:69	arg1	characterization					29:44	characterization	29:44	characterization	29:44	Study on the fabrication and characterization of tip-loaded dissolving microneedles for transdermal drug delivery.
33059004	0	66	theme	dissolving	60:69	arg1	fabrication					13:23	fabrication	13:23	fabrication	13:23	Study on the fabrication and characterization of tip-loaded dissolving microneedles for transdermal drug delivery.
33059004	8	67	theme	dissolving	1261:1270	arg1	microneedles					1272:1283	our fabricated tip-loaded dissolving microneedles	1235:1283	our fabricated tip-loaded dissolving microneedles	1235:1283	Moreover, our fabricated tip-loaded dissolving microneedles can greatly reduce the drug waste cause by unused backing layer in normal microneedles and realize a 30% enhancement of drug delivery efficiency after puncture treatment.
33059004	8	68	theme	waste	1313:1317	arg1	cause					1319:1323	the drug waste cause	1304:1323	the drug waste cause	1304:1323	Moreover, our fabricated tip-loaded dissolving microneedles can greatly reduce the drug waste cause by unused backing layer in normal microneedles and realize a 30% enhancement of drug delivery efficiency after puncture treatment.
33059004	3	69	theme	pure	509:512	arg1	material					525:532	pure dissolving material	509:532	pure dissolving material	509:532	The tip layer of the microneedles carried model drug, while the backing layer was fabricated with pure dissolving material.
33059004	4	70	theme	base	612:615	arg1	polyvinylpyrrolidone					554:573	polyvinylpyrrolidone	554:573	polyvinylpyrrolidone	554:573	Polyvinyl alcohol, polyvinylpyrrolidone and hyaluronic acid were used as the base materials to fabricate the dissolving layers of the microneedle patches.
33059004	4	70	theme	base	612:615	arg1	acid					590:593	hyaluronic acid	579:593	hyaluronic acid	579:593	Polyvinyl alcohol, polyvinylpyrrolidone and hyaluronic acid were used as the base materials to fabricate the dissolving layers of the microneedle patches.
33059004	4	70	theme	base	612:615	arg1	alcohol					545:551	Polyvinyl alcohol	535:551	Polyvinyl alcohol	535:551	Polyvinyl alcohol, polyvinylpyrrolidone and hyaluronic acid were used as the base materials to fabricate the dissolving layers of the microneedle patches.
33059004	4	70	theme	base	612:615	arg1	materials					617:625	the base materials	608:625	the base materials	608:625	Polyvinyl alcohol, polyvinylpyrrolidone and hyaluronic acid were used as the base materials to fabricate the dissolving layers of the microneedle patches.
32781127	2	0	theme	bone	646:649	arg1	engineering					651:661	bone engineering	646:661	bone engineering	646:661	Inspired by the natural extracellular matrix of bone tissue, organic-inorganic composite scaffolds with three-dimensional (3D) porous structures, sufficient mechanical properties, excellent cytocompatibility, osteoconductivity, and osteogenic potential have received considerable attention within the field of bone engineering.
32781127	3	1	theme	-crosslinked	707:718	arg1	cellulose					733:741	-crosslinked hydroxyethyl cellulose	707:741	a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp)	678:864	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	3	1	theme	-crosslinked	707:718	arg1	HEC					744:746	HEC	744:746	HEC	744:746	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	5	2	theme	HAp	1256:1258	arg1	coating					1260:1266	a biomimetic HAp coating	1243:1266	a biomimetic HAp coating on their surfaces	1243:1284	The results indicated that the prepared scaffolds exhibited an interconnected porous structure, a biomimetic HAp coating on their surfaces, improved mechanical properties in compression and a controllable degradation rate.
32781127	5	2	theme	HAp	1256:1258	arg1	structure					1232:1240	an interconnected porous structure	1207:1240	an interconnected porous structure	1207:1240	The results indicated that the prepared scaffolds exhibited an interconnected porous structure, a biomimetic HAp coating on their surfaces, improved mechanical properties in compression and a controllable degradation rate.
32781127	8	3	theme	70	1883:1884	arg1	%					1885:1885	%	1885:1885	%	1885:1885	Consistently, in vitro osteogenic differentiation studies showed that EHSS/HAp with 70% SPI content can significantly accelerate the expression of osteogenesis-related genes (Col-1, Runx2, OPN, and OCN) during osteogenic differentiation of MC3T3-E1 cells.
32781127	12	4	theme	defect	2780:2785	arg1	repair					2787:2792	successful bone defect repair	2764:2792	successful bone defect repair	2764:2792	Collectively, our results demonstrate the potential of EHSS/HAp scaffolds with 70% SPI for successful bone defect repair and regeneration.
32781127	1	5	used	utilized	174:181	arg2	strategies					153:162	various strategies	145:162	various strategies	145:162	Although various strategies have been utilized to accelerate bone regeneration in bone tissue engineering (BTE), the treatment and repair of large bone defects remains a clinical challenge worldwide.
32781127	9	6	with	EHSS/HAp	2145:2152	arg1	SPI					2163:2165	70% SPI	2159:2165	70% SPI	2159:2165	Furthermore, when applied to the repair of critical-sized cranial defects in a rat model, EHSS/HAp with 70% SPI was capable of significantly promoting tissue regeneration and integration with native bone tissue.
32781127	11	7	theme	%	2614:2614	arg1	SPI					2616:2618	70% SPI	2612:2618	70% SPI	2612:2618	Hematoxylin and eosin (H&E) staining and Masson's trichrome staining of histological sections further confirmed that EHSS/HAp with 70% SPI markedly promoted new bone formation and maturation.
32781127	3	8	theme	/soy	748:751	arg1	EHSS					805:808	EHSS	805:808	EHSS	805:808	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	3	8	theme	/soy	748:751	arg1	scaffold					795:802	/soy protein isolate (SPI) porous bi-component scaffold	748:802	a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp)	678:864	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	3	9	with	epichlorohydrin	686:700	arg1	EHSS/HAp					856:863	EHSS/HAp	856:863	EHSS/HAp	856:863	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	3	9	with	epichlorohydrin	686:700	arg1	functionalization					837:853	hydroxyapatite (HAp) functionalization	816:853	hydroxyapatite (HAp) functionalization (EHSS/HAp)	816:864	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	8	10	theme	differentiation	1833:1847	arg1	studies					1849:1855	in vitro osteogenic differentiation studies	1813:1855	in vitro osteogenic differentiation studies	1813:1855	Consistently, in vitro osteogenic differentiation studies showed that EHSS/HAp with 70% SPI content can significantly accelerate the expression of osteogenesis-related genes (Col-1, Runx2, OPN, and OCN) during osteogenic differentiation of MC3T3-E1 cells.
32781127	10	11	theme	defect	2345:2350	arg1	site					2352:2355	the bone defect site	2336:2355	the bone defect site	2336:2355	Microscopic computed tomography (micro-CT) results demonstrated that the bone defect site was nearly occupied with newly formed bone at 12 weeks after implantation of EHSS/HAp with 70% SPI content into the defect.
32781127	10	12	used	occupied	2368:2375	arg2	site					2352:2355	the bone defect site	2336:2355	the bone defect site	2336:2355	Microscopic computed tomography (micro-CT) results demonstrated that the bone defect site was nearly occupied with newly formed bone at 12 weeks after implantation of EHSS/HAp with 70% SPI content into the defect.
32781127	5	13	theme	biomimetic	1245:1254	arg1	coating					1260:1266	a biomimetic HAp coating	1243:1266	a biomimetic HAp coating on their surfaces	1243:1284	The results indicated that the prepared scaffolds exhibited an interconnected porous structure, a biomimetic HAp coating on their surfaces, improved mechanical properties in compression and a controllable degradation rate.
32781127	5	13	theme	biomimetic	1245:1254	arg1	structure					1232:1240	an interconnected porous structure	1207:1240	an interconnected porous structure	1207:1240	The results indicated that the prepared scaffolds exhibited an interconnected porous structure, a biomimetic HAp coating on their surfaces, improved mechanical properties in compression and a controllable degradation rate.
32781127	2	14	with	scaffolds	425:433	arg1	potential					579:587	osteogenic potential	568:587	osteogenic potential	568:587	Inspired by the natural extracellular matrix of bone tissue, organic-inorganic composite scaffolds with three-dimensional (3D) porous structures, sufficient mechanical properties, excellent cytocompatibility, osteoconductivity, and osteogenic potential have received considerable attention within the field of bone engineering.
32781127	2	14	with	scaffolds	425:433	arg1	structures					470:479	three-dimensional (3D) porous structures	440:479	three-dimensional (3D) porous structures	440:479	Inspired by the natural extracellular matrix of bone tissue, organic-inorganic composite scaffolds with three-dimensional (3D) porous structures, sufficient mechanical properties, excellent cytocompatibility, osteoconductivity, and osteogenic potential have received considerable attention within the field of bone engineering.
32781127	2	14	with	scaffolds	425:433	arg1	osteoconductivity					545:561	osteoconductivity	545:561	osteoconductivity	545:561	Inspired by the natural extracellular matrix of bone tissue, organic-inorganic composite scaffolds with three-dimensional (3D) porous structures, sufficient mechanical properties, excellent cytocompatibility, osteoconductivity, and osteogenic potential have received considerable attention within the field of bone engineering.
32781127	2	14	with	scaffolds	425:433	arg1	properties					504:513	sufficient mechanical properties	482:513	sufficient mechanical properties	482:513	Inspired by the natural extracellular matrix of bone tissue, organic-inorganic composite scaffolds with three-dimensional (3D) porous structures, sufficient mechanical properties, excellent cytocompatibility, osteoconductivity, and osteogenic potential have received considerable attention within the field of bone engineering.
32781127	2	14	with	scaffolds	425:433	arg1	cytocompatibility					526:542	excellent cytocompatibility	516:542	excellent cytocompatibility	516:542	Inspired by the natural extracellular matrix of bone tissue, organic-inorganic composite scaffolds with three-dimensional (3D) porous structures, sufficient mechanical properties, excellent cytocompatibility, osteoconductivity, and osteogenic potential have received considerable attention within the field of bone engineering.
32781127	3	15	theme	SPI	770:772	arg1	EHSS					805:808	EHSS	805:808	EHSS	805:808	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	3	15	theme	SPI	770:772	arg1	scaffold					795:802	/soy protein isolate (SPI) porous bi-component scaffold	748:802	a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp)	678:864	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	8	16	theme	osteogenesis-related	1946:1965	arg1	OPN					1988:1990	OPN	1988:1990	OPN	1988:1990	Consistently, in vitro osteogenic differentiation studies showed that EHSS/HAp with 70% SPI content can significantly accelerate the expression of osteogenesis-related genes (Col-1, Runx2, OPN, and OCN) during osteogenic differentiation of MC3T3-E1 cells.
32781127	8	16	theme	osteogenesis-related	1946:1965	arg1	OCN					1997:1999	OCN	1997:1999	OCN	1997:1999	Consistently, in vitro osteogenic differentiation studies showed that EHSS/HAp with 70% SPI content can significantly accelerate the expression of osteogenesis-related genes (Col-1, Runx2, OPN, and OCN) during osteogenic differentiation of MC3T3-E1 cells.
32781127	8	16	theme	osteogenesis-related	1946:1965	arg1	Runx2					1981:1985	Runx2	1981:1985	Runx2	1981:1985	Consistently, in vitro osteogenic differentiation studies showed that EHSS/HAp with 70% SPI content can significantly accelerate the expression of osteogenesis-related genes (Col-1, Runx2, OPN, and OCN) during osteogenic differentiation of MC3T3-E1 cells.
32781127	8	16	theme	osteogenesis-related	1946:1965	arg1	Col-1					1974:1978	Col-1	1974:1978	Col-1	1974:1978	Consistently, in vitro osteogenic differentiation studies showed that EHSS/HAp with 70% SPI content can significantly accelerate the expression of osteogenesis-related genes (Col-1, Runx2, OPN, and OCN) during osteogenic differentiation of MC3T3-E1 cells.
32781127	8	16	theme	osteogenesis-related	1946:1965	arg1	genes					1967:1971	osteogenesis-related genes	1946:1971	osteogenesis-related genes (Col-1, Runx2, OPN, and OCN)	1946:2000	Consistently, in vitro osteogenic differentiation studies showed that EHSS/HAp with 70% SPI content can significantly accelerate the expression of osteogenesis-related genes (Col-1, Runx2, OPN, and OCN) during osteogenic differentiation of MC3T3-E1 cells.
32781127	7	17	theme	%	1678:1678	arg1	content					1684:1690	70% SPI content	1676:1690	70% SPI content	1676:1690	In vitro cell culture experiments indicated that the EHSS/HAp with 70% SPI content showed improved cytocompatibility and was suitable for MC3T3-E1 cell attachment, proliferation and growth.
32781127	4	18	theme	scaffold	1137:1144	arg1	properties					1098:1107	mechanical properties	1087:1107	mechanical properties	1087:1107	Systematic characterization experiments were performed to assess the morphology, HAp-forming properties, mechanical properties and degradation rate of the scaffold.
32781127	4	18	theme	scaffold	1137:1144	arg1	rate					1125:1128	degradation rate	1113:1128	degradation rate	1113:1128	Systematic characterization experiments were performed to assess the morphology, HAp-forming properties, mechanical properties and degradation rate of the scaffold.
32781127	4	18	theme	scaffold	1137:1144	arg1	morphology					1051:1060	morphology	1051:1060	morphology	1051:1060	Systematic characterization experiments were performed to assess the morphology, HAp-forming properties, mechanical properties and degradation rate of the scaffold.
32781127	4	18	theme	scaffold	1137:1144	arg1	properties					1075:1084	HAp-forming properties	1063:1084	HAp-forming properties	1063:1084	Systematic characterization experiments were performed to assess the morphology, HAp-forming properties, mechanical properties and degradation rate of the scaffold.
32781127	3	19	theme	isolate	761:767	arg1	EHSS					805:808	EHSS	805:808	EHSS	805:808	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	3	19	theme	isolate	761:767	arg1	scaffold					795:802	/soy protein isolate (SPI) porous bi-component scaffold	748:802	a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp)	678:864	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	7	20	dep	In	1609:1610	arg1	vitro					1612:1616	vitro	1612:1616	vitro	1612:1616	In vitro cell culture experiments indicated that the EHSS/HAp with 70% SPI content showed improved cytocompatibility and was suitable for MC3T3-E1 cell attachment, proliferation and growth.
32781127	1	21	theme	bone	218:221	arg1	BTE					243:245	BTE	243:245	BTE	243:245	Although various strategies have been utilized to accelerate bone regeneration in bone tissue engineering (BTE), the treatment and repair of large bone defects remains a clinical challenge worldwide.
32781127	1	21	theme	bone	218:221	arg1	engineering					230:240	bone tissue engineering	218:240	bone tissue engineering (BTE)	218:246	Although various strategies have been utilized to accelerate bone regeneration in bone tissue engineering (BTE), the treatment and repair of large bone defects remains a clinical challenge worldwide.
32781127	2	22	theme	osteogenic	568:577	arg1	potential					579:587	osteogenic potential	568:587	osteogenic potential	568:587	Inspired by the natural extracellular matrix of bone tissue, organic-inorganic composite scaffolds with three-dimensional (3D) porous structures, sufficient mechanical properties, excellent cytocompatibility, osteoconductivity, and osteogenic potential have received considerable attention within the field of bone engineering.
32781127	10	23	theme	bone	2395:2398	arg1	12 weeks					2403:2410	newly formed bone at 12 weeks	2382:2410	newly formed bone at 12 weeks after implantation of EHSS/HAp with 70% SPI content into the defect	2382:2478	Microscopic computed tomography (micro-CT) results demonstrated that the bone defect site was nearly occupied with newly formed bone at 12 weeks after implantation of EHSS/HAp with 70% SPI content into the defect.
32781127	11	24	theme	trichrome	2531:2539	arg1	staining					2541:2548	Masson's trichrome staining	2522:2548	Masson's trichrome staining	2522:2548	Hematoxylin and eosin (H&E) staining and Masson's trichrome staining of histological sections further confirmed that EHSS/HAp with 70% SPI markedly promoted new bone formation and maturation.
32781127	9	25	theme	70	2159:2160	arg1	%					2161:2161	%	2161:2161	%	2161:2161	Furthermore, when applied to the repair of critical-sized cranial defects in a rat model, EHSS/HAp with 70% SPI was capable of significantly promoting tissue regeneration and integration with native bone tissue.
32781127	3	26	theme	porous	775:780	arg1	EHSS					805:808	EHSS	805:808	EHSS	805:808	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	3	26	theme	porous	775:780	arg1	scaffold					795:802	/soy protein isolate (SPI) porous bi-component scaffold	748:802	a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp)	678:864	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	8	27	theme	in	1813:1814	arg1	studies					1849:1855	in vitro osteogenic differentiation studies	1813:1855	in vitro osteogenic differentiation studies	1813:1855	Consistently, in vitro osteogenic differentiation studies showed that EHSS/HAp with 70% SPI content can significantly accelerate the expression of osteogenesis-related genes (Col-1, Runx2, OPN, and OCN) during osteogenic differentiation of MC3T3-E1 cells.
32781127	2	28	theme	considerable	603:614	arg1	attention					616:624	considerable attention	603:624	considerable attention	603:624	Inspired by the natural extracellular matrix of bone tissue, organic-inorganic composite scaffolds with three-dimensional (3D) porous structures, sufficient mechanical properties, excellent cytocompatibility, osteoconductivity, and osteogenic potential have received considerable attention within the field of bone engineering.
32781127	9	29	theme	native	2247:2252	arg1	tissue					2259:2264	native bone tissue	2247:2264	native bone tissue	2247:2264	Furthermore, when applied to the repair of critical-sized cranial defects in a rat model, EHSS/HAp with 70% SPI was capable of significantly promoting tissue regeneration and integration with native bone tissue.
32781127	8	30	theme	osteogenic	1822:1831	arg1	studies					1849:1855	in vitro osteogenic differentiation studies	1813:1855	in vitro osteogenic differentiation studies	1813:1855	Consistently, in vitro osteogenic differentiation studies showed that EHSS/HAp with 70% SPI content can significantly accelerate the expression of osteogenesis-related genes (Col-1, Runx2, OPN, and OCN) during osteogenic differentiation of MC3T3-E1 cells.
32781127	1	31	theme	various	145:151	arg1	strategies					153:162	various strategies	145:162	various strategies	145:162	Although various strategies have been utilized to accelerate bone regeneration in bone tissue engineering (BTE), the treatment and repair of large bone defects remains a clinical challenge worldwide.
32781127	7	32	theme	culture	1623:1629	arg1	experiments					1631:1641	In vitro cell culture experiments	1609:1641	In vitro cell culture experiments	1609:1641	In vitro cell culture experiments indicated that the EHSS/HAp with 70% SPI content showed improved cytocompatibility and was suitable for MC3T3-E1 cell attachment, proliferation and growth.
32781127	4	33	theme	characterization	993:1008	arg1	experiments					1010:1020	Systematic characterization experiments	982:1020	Systematic characterization experiments	982:1020	Systematic characterization experiments were performed to assess the morphology, HAp-forming properties, mechanical properties and degradation rate of the scaffold.
32781127	1	34	dep	treatment	253:261	arg1	the					249:251	the	249:251	the	249:251	Although various strategies have been utilized to accelerate bone regeneration in bone tissue engineering (BTE), the treatment and repair of large bone defects remains a clinical challenge worldwide.
32781127	11	35	theme	Hematoxylin	2481:2491	arg1	staining					2509:2516	Hematoxylin and eosin (H&E) staining	2481:2516	Hematoxylin and eosin (H&E) staining	2481:2516	Hematoxylin and eosin (H&E) staining and Masson's trichrome staining of histological sections further confirmed that EHSS/HAp with 70% SPI markedly promoted new bone formation and maturation.
32781127	5	36	theme	degradation	1352:1362	arg1	rate					1364:1367	a controllable degradation rate	1337:1367	a controllable degradation rate	1337:1367	The results indicated that the prepared scaffolds exhibited an interconnected porous structure, a biomimetic HAp coating on their surfaces, improved mechanical properties in compression and a controllable degradation rate.
32781127	0	37	theme	scaffolds	78:86	arg1	mineralization					11:24	Biomimetic mineralization	0:24	Biomimetic mineralization of novel hydroxyethyl cellulose/soy protein isolate scaffolds	0:86	Biomimetic mineralization of novel hydroxyethyl cellulose/soy protein isolate scaffolds promote bone regeneration in vitro and in vivo.
32781127	4	38	theme	HAp-forming	1063:1073	arg1	properties					1075:1084	HAp-forming properties	1063:1084	HAp-forming properties	1063:1084	Systematic characterization experiments were performed to assess the morphology, HAp-forming properties, mechanical properties and degradation rate of the scaffold.
32781127	6	39	theme	spectroscopy	1586:1597	arg1	analysis					1599:1606	energy dispersive X-ray spectroscopy analysis	1562:1606	energy dispersive X-ray spectroscopy analysis	1562:1606	In particular, semiquantitative analysis showed that the calcium/phosphorus (Ca/P) ratio of EHSS/HAp with 70% SPI content (1.65) was similar to that of natural bone tissue (1.67) according to energy dispersive X-ray spectroscopy analysis.
32781127	3	40	theme	bone	886:889	arg1	repair					898:903	bone defect repair	886:903	bone defect repair	886:903	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	12	41	with	potential	2715:2723	arg1	SPI					2756:2758	70% SPI	2752:2758	70% SPI	2752:2758	Collectively, our results demonstrate the potential of EHSS/HAp scaffolds with 70% SPI for successful bone defect repair and regeneration.
32781127	9	42	theme	%	2161:2161	arg1	SPI					2163:2165	70% SPI	2159:2165	70% SPI	2159:2165	Furthermore, when applied to the repair of critical-sized cranial defects in a rat model, EHSS/HAp with 70% SPI was capable of significantly promoting tissue regeneration and integration with native bone tissue.
32781127	0	43	theme	bone	96:99	arg1	regeneration					101:112	bone regeneration	96:112	bone regeneration	96:112	Biomimetic mineralization of novel hydroxyethyl cellulose/soy protein isolate scaffolds promote bone regeneration in vitro and in vivo.
32781127	12	44	theme	bone	2775:2778	arg1	repair					2787:2792	successful bone defect repair	2764:2792	successful bone defect repair	2764:2792	Collectively, our results demonstrate the potential of EHSS/HAp scaffolds with 70% SPI for successful bone defect repair and regeneration.
32781127	10	45	theme	70	2448:2449	arg1	%					2450:2450	%	2450:2450	%	2450:2450	Microscopic computed tomography (micro-CT) results demonstrated that the bone defect site was nearly occupied with newly formed bone at 12 weeks after implantation of EHSS/HAp with 70% SPI content into the defect.
32781127	11	46	theme	eosin	2497:2501	arg1	staining					2509:2516	Hematoxylin and eosin (H&E) staining	2481:2516	Hematoxylin and eosin (H&E) staining	2481:2516	Hematoxylin and eosin (H&E) staining and Masson's trichrome staining of histological sections further confirmed that EHSS/HAp with 70% SPI markedly promoted new bone formation and maturation.
32781127	6	47	theme	calcium/phosphorus	1427:1444	arg1	similar					1503:1509	similar	1503:1509	similar	1503:1509	In particular, semiquantitative analysis showed that the calcium/phosphorus (Ca/P) ratio of EHSS/HAp with 70% SPI content (1.65) was similar to that of natural bone tissue (1.67) according to energy dispersive X-ray spectroscopy analysis.
32781127	6	47	theme	calcium/phosphorus	1427:1444	arg1	ratio					1453:1457	the calcium/phosphorus (Ca/P) ratio	1423:1457	the calcium/phosphorus (Ca/P) ratio of EHSS/HAp with 70% SPI content (1.65)	1423:1497	In particular, semiquantitative analysis showed that the calcium/phosphorus (Ca/P) ratio of EHSS/HAp with 70% SPI content (1.65) was similar to that of natural bone tissue (1.67) according to energy dispersive X-ray spectroscopy analysis.
32781127	10	48	theme	EHSS/HAp	2434:2441	arg1	implantation					2418:2429	implantation	2418:2429	implantation of EHSS/HAp with 70% SPI content into the defect	2418:2478	Microscopic computed tomography (micro-CT) results demonstrated that the bone defect site was nearly occupied with newly formed bone at 12 weeks after implantation of EHSS/HAp with 70% SPI content into the defect.
32781127	6	49	theme	dispersive	1569:1578	arg1	spectroscopy					1586:1597	energy dispersive X-ray spectroscopy	1562:1597	energy dispersive X-ray spectroscopy analysis	1562:1606	In particular, semiquantitative analysis showed that the calcium/phosphorus (Ca/P) ratio of EHSS/HAp with 70% SPI content (1.65) was similar to that of natural bone tissue (1.67) according to energy dispersive X-ray spectroscopy analysis.
32781127	8	50	theme	MC3T3-E1	2039:2046	arg1	cells					2048:2052	MC3T3-E1 cells	2039:2052	MC3T3-E1 cells	2039:2052	Consistently, in vitro osteogenic differentiation studies showed that EHSS/HAp with 70% SPI content can significantly accelerate the expression of osteogenesis-related genes (Col-1, Runx2, OPN, and OCN) during osteogenic differentiation of MC3T3-E1 cells.
32781127	0	51	theme	cellulose/soy	48:60	arg1	scaffolds					78:86	novel hydroxyethyl cellulose/soy protein isolate scaffolds	29:86	novel hydroxyethyl cellulose/soy protein isolate scaffolds	29:86	Biomimetic mineralization of novel hydroxyethyl cellulose/soy protein isolate scaffolds promote bone regeneration in vitro and in vivo.
32781127	7	52	with	EHSS/HAp	1662:1669	arg1	content					1684:1690	70% SPI content	1676:1690	70% SPI content	1676:1690	In vitro cell culture experiments indicated that the EHSS/HAp with 70% SPI content showed improved cytocompatibility and was suitable for MC3T3-E1 cell attachment, proliferation and growth.
32781127	9	53	theme	bone	2254:2257	arg1	tissue					2259:2264	native bone tissue	2247:2264	native bone tissue	2247:2264	Furthermore, when applied to the repair of critical-sized cranial defects in a rat model, EHSS/HAp with 70% SPI was capable of significantly promoting tissue regeneration and integration with native bone tissue.
32781127	7	54	theme	improved	1699:1706	arg1	cytocompatibility					1708:1724	improved cytocompatibility	1699:1724	improved cytocompatibility	1699:1724	In vitro cell culture experiments indicated that the EHSS/HAp with 70% SPI content showed improved cytocompatibility and was suitable for MC3T3-E1 cell attachment, proliferation and growth.
32781127	2	55	theme	excellent	516:524	arg1	cytocompatibility					526:542	excellent cytocompatibility	516:542	excellent cytocompatibility	516:542	Inspired by the natural extracellular matrix of bone tissue, organic-inorganic composite scaffolds with three-dimensional (3D) porous structures, sufficient mechanical properties, excellent cytocompatibility, osteoconductivity, and osteogenic potential have received considerable attention within the field of bone engineering.
32781127	2	56	theme	bone	384:387	arg1	tissue					389:394	bone tissue	384:394	bone tissue	384:394	Inspired by the natural extracellular matrix of bone tissue, organic-inorganic composite scaffolds with three-dimensional (3D) porous structures, sufficient mechanical properties, excellent cytocompatibility, osteoconductivity, and osteogenic potential have received considerable attention within the field of bone engineering.
32781127	0	57	theme	Biomimetic	0:9	arg1	mineralization					11:24	Biomimetic mineralization	0:24	Biomimetic mineralization of novel hydroxyethyl cellulose/soy protein isolate scaffolds	0:86	Biomimetic mineralization of novel hydroxyethyl cellulose/soy protein isolate scaffolds promote bone regeneration in vitro and in vivo.
32781127	5	58	theme	porous	1225:1230	arg1	coating					1260:1266	a biomimetic HAp coating	1243:1266	a biomimetic HAp coating on their surfaces	1243:1284	The results indicated that the prepared scaffolds exhibited an interconnected porous structure, a biomimetic HAp coating on their surfaces, improved mechanical properties in compression and a controllable degradation rate.
32781127	5	58	theme	porous	1225:1230	arg1	structure					1232:1240	an interconnected porous structure	1207:1240	an interconnected porous structure	1207:1240	The results indicated that the prepared scaffolds exhibited an interconnected porous structure, a biomimetic HAp coating on their surfaces, improved mechanical properties in compression and a controllable degradation rate.
32781127	5	58	theme	porous	1225:1230	arg1	properties					1307:1316	improved mechanical properties	1287:1316	improved mechanical properties in compression and a controllable degradation rate	1287:1367	The results indicated that the prepared scaffolds exhibited an interconnected porous structure, a biomimetic HAp coating on their surfaces, improved mechanical properties in compression and a controllable degradation rate.
32781127	11	59	theme	sections	2566:2573	arg1	staining					2541:2548	Masson's trichrome staining	2522:2548	Masson's trichrome staining	2522:2548	Hematoxylin and eosin (H&E) staining and Masson's trichrome staining of histological sections further confirmed that EHSS/HAp with 70% SPI markedly promoted new bone formation and maturation.
32781127	11	59	theme	sections	2566:2573	arg1	staining					2509:2516	Hematoxylin and eosin (H&E) staining	2481:2516	Hematoxylin and eosin (H&E) staining	2481:2516	Hematoxylin and eosin (H&E) staining and Masson's trichrome staining of histological sections further confirmed that EHSS/HAp with 70% SPI markedly promoted new bone formation and maturation.
32781127	12	60	theme	70	2752:2753	arg1	%					2754:2754	%	2754:2754	%	2754:2754	Collectively, our results demonstrate the potential of EHSS/HAp scaffolds with 70% SPI for successful bone defect repair and regeneration.
32781127	9	61	theme	critical-sized	2098:2111	arg1	defects					2121:2127	critical-sized cranial defects	2098:2127	critical-sized cranial defects in a rat model	2098:2142	Furthermore, when applied to the repair of critical-sized cranial defects in a rat model, EHSS/HAp with 70% SPI was capable of significantly promoting tissue regeneration and integration with native bone tissue.
32781127	10	62	theme	SPI	2452:2454	arg1	content					2456:2462	70% SPI content	2448:2462	70% SPI content into the defect	2448:2478	Microscopic computed tomography (micro-CT) results demonstrated that the bone defect site was nearly occupied with newly formed bone at 12 weeks after implantation of EHSS/HAp with 70% SPI content into the defect.
32781127	10	63	theme	Microscopic	2267:2277	arg1	results					2310:2316	Microscopic computed tomography (micro-CT) results	2267:2316	Microscopic computed tomography (micro-CT) results	2267:2316	Microscopic computed tomography (micro-CT) results demonstrated that the bone defect site was nearly occupied with newly formed bone at 12 weeks after implantation of EHSS/HAp with 70% SPI content into the defect.
32781127	6	64	theme	SPI	1480:1482	arg1	content					1484:1490	70% SPI content	1476:1490	70% SPI content	1476:1490	In particular, semiquantitative analysis showed that the calcium/phosphorus (Ca/P) ratio of EHSS/HAp with 70% SPI content (1.65) was similar to that of natural bone tissue (1.67) according to energy dispersive X-ray spectroscopy analysis.
32781127	1	65	theme	bone	283:286	arg1	defects					288:294	large bone defects	277:294	large bone defects	277:294	Although various strategies have been utilized to accelerate bone regeneration in bone tissue engineering (BTE), the treatment and repair of large bone defects remains a clinical challenge worldwide.
32781127	7	66	theme	SPI	1680:1682	arg1	content					1684:1690	70% SPI content	1676:1690	70% SPI content	1676:1690	In vitro cell culture experiments indicated that the EHSS/HAp with 70% SPI content showed improved cytocompatibility and was suitable for MC3T3-E1 cell attachment, proliferation and growth.
32781127	8	67	dep	genes	1967:1971	arg1	OPN					1988:1990	OPN	1988:1990	OPN	1988:1990	Consistently, in vitro osteogenic differentiation studies showed that EHSS/HAp with 70% SPI content can significantly accelerate the expression of osteogenesis-related genes (Col-1, Runx2, OPN, and OCN) during osteogenic differentiation of MC3T3-E1 cells.
32781127	8	67	dep	genes	1967:1971	arg1	OCN					1997:1999	OCN	1997:1999	OCN	1997:1999	Consistently, in vitro osteogenic differentiation studies showed that EHSS/HAp with 70% SPI content can significantly accelerate the expression of osteogenesis-related genes (Col-1, Runx2, OPN, and OCN) during osteogenic differentiation of MC3T3-E1 cells.
32781127	8	67	dep	genes	1967:1971	arg1	Runx2					1981:1985	Runx2	1981:1985	Runx2	1981:1985	Consistently, in vitro osteogenic differentiation studies showed that EHSS/HAp with 70% SPI content can significantly accelerate the expression of osteogenesis-related genes (Col-1, Runx2, OPN, and OCN) during osteogenic differentiation of MC3T3-E1 cells.
32781127	8	67	dep	genes	1967:1971	arg1	Col-1					1974:1978	Col-1	1974:1978	Col-1	1974:1978	Consistently, in vitro osteogenic differentiation studies showed that EHSS/HAp with 70% SPI content can significantly accelerate the expression of osteogenesis-related genes (Col-1, Runx2, OPN, and OCN) during osteogenic differentiation of MC3T3-E1 cells.
32781127	8	67	dep	genes	1967:1971	arg1	genes					1967:1971	osteogenesis-related genes	1946:1971	osteogenesis-related genes (Col-1, Runx2, OPN, and OCN)	1946:2000	Consistently, in vitro osteogenic differentiation studies showed that EHSS/HAp with 70% SPI content can significantly accelerate the expression of osteogenesis-related genes (Col-1, Runx2, OPN, and OCN) during osteogenic differentiation of MC3T3-E1 cells.
32781127	2	68	theme	sufficient	482:491	arg1	properties					504:513	sufficient mechanical properties	482:513	sufficient mechanical properties	482:513	Inspired by the natural extracellular matrix of bone tissue, organic-inorganic composite scaffolds with three-dimensional (3D) porous structures, sufficient mechanical properties, excellent cytocompatibility, osteoconductivity, and osteogenic potential have received considerable attention within the field of bone engineering.
32781127	11	69	theme	bone	2642:2645	arg1	formation					2647:2655	new bone formation	2638:2655	new bone formation	2638:2655	Hematoxylin and eosin (H&E) staining and Masson's trichrome staining of histological sections further confirmed that EHSS/HAp with 70% SPI markedly promoted new bone formation and maturation.
32781127	3	70	theme	novel	680:684	arg1	ECH					703:705	ECH	703:705	ECH	703:705	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	3	70	theme	novel	680:684	arg1	epichlorohydrin					686:700	a novel epichlorohydrin	678:700	a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp)	678:864	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	2	71	theme	composite	415:423	arg1	scaffolds					425:433	organic-inorganic composite scaffolds	397:433	organic-inorganic composite scaffolds with three-dimensional (3D) porous structures, sufficient mechanical properties, excellent cytocompatibility, osteoconductivity, and osteogenic potential	397:587	Inspired by the natural extracellular matrix of bone tissue, organic-inorganic composite scaffolds with three-dimensional (3D) porous structures, sufficient mechanical properties, excellent cytocompatibility, osteoconductivity, and osteogenic potential have received considerable attention within the field of bone engineering.
32781127	0	72	theme	hydroxyethyl	35:46	arg1	scaffolds					78:86	novel hydroxyethyl cellulose/soy protein isolate scaffolds	29:86	novel hydroxyethyl cellulose/soy protein isolate scaffolds	29:86	Biomimetic mineralization of novel hydroxyethyl cellulose/soy protein isolate scaffolds promote bone regeneration in vitro and in vivo.
32781127	6	73	theme	70	1476:1477	arg1	%					1478:1478	%	1478:1478	%	1478:1478	In particular, semiquantitative analysis showed that the calcium/phosphorus (Ca/P) ratio of EHSS/HAp with 70% SPI content (1.65) was similar to that of natural bone tissue (1.67) according to energy dispersive X-ray spectroscopy analysis.
32781127	3	74	theme	mineralization	966:979	arg1	combination					913:923	the combination	909:923	the combination of lyophilization and in situ biomimetic mineralization	909:979	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	9	75	theme	defects	2121:2127	arg1	repair					2088:2093	the repair	2084:2093	the repair of critical-sized cranial defects in a rat model	2084:2142	Furthermore, when applied to the repair of critical-sized cranial defects in a rat model, EHSS/HAp with 70% SPI was capable of significantly promoting tissue regeneration and integration with native bone tissue.
32781127	0	76	theme	protein	62:68	arg1	scaffolds					78:86	novel hydroxyethyl cellulose/soy protein isolate scaffolds	29:86	novel hydroxyethyl cellulose/soy protein isolate scaffolds	29:86	Biomimetic mineralization of novel hydroxyethyl cellulose/soy protein isolate scaffolds promote bone regeneration in vitro and in vivo.
32781127	1	77	theme	clinical	306:313	arg1	worldwide					325:333	a clinical challenge worldwide	304:333	a clinical challenge worldwide	304:333	Although various strategies have been utilized to accelerate bone regeneration in bone tissue engineering (BTE), the treatment and repair of large bone defects remains a clinical challenge worldwide.
32781127	9	78	from	repair	2088:2093	arg1	model					2138:2142	a rat model	2132:2142	a rat model	2132:2142	Furthermore, when applied to the repair of critical-sized cranial defects in a rat model, EHSS/HAp with 70% SPI was capable of significantly promoting tissue regeneration and integration with native bone tissue.
32781127	6	79	theme	natural	1522:1528	arg1	1.67					1543:1546	1.67	1543:1546	1.67	1543:1546	In particular, semiquantitative analysis showed that the calcium/phosphorus (Ca/P) ratio of EHSS/HAp with 70% SPI content (1.65) was similar to that of natural bone tissue (1.67) according to energy dispersive X-ray spectroscopy analysis.
32781127	6	79	theme	natural	1522:1528	arg1	tissue					1535:1540	natural bone tissue	1522:1540	natural bone tissue (1.67) according to energy dispersive X-ray spectroscopy analysis	1522:1606	In particular, semiquantitative analysis showed that the calcium/phosphorus (Ca/P) ratio of EHSS/HAp with 70% SPI content (1.65) was similar to that of natural bone tissue (1.67) according to energy dispersive X-ray spectroscopy analysis.
32781127	11	80	theme	70	2612:2613	arg1	%					2614:2614	%	2614:2614	%	2614:2614	Hematoxylin and eosin (H&E) staining and Masson's trichrome staining of histological sections further confirmed that EHSS/HAp with 70% SPI markedly promoted new bone formation and maturation.
32781127	11	81	with	EHSS/HAp	2598:2605	arg1	SPI					2616:2618	70% SPI	2612:2618	70% SPI	2612:2618	Hematoxylin and eosin (H&E) staining and Masson's trichrome staining of histological sections further confirmed that EHSS/HAp with 70% SPI markedly promoted new bone formation and maturation.
32781127	4	82	theme	mechanical	1087:1096	arg1	properties					1098:1107	mechanical properties	1087:1107	mechanical properties	1087:1107	Systematic characterization experiments were performed to assess the morphology, HAp-forming properties, mechanical properties and degradation rate of the scaffold.
32781127	12	83	theme	successful	2764:2773	arg1	repair					2787:2792	successful bone defect repair	2764:2792	successful bone defect repair	2764:2792	Collectively, our results demonstrate the potential of EHSS/HAp scaffolds with 70% SPI for successful bone defect repair and regeneration.
32781127	2	84	theme	natural	352:358	arg1	matrix					374:379	the natural extracellular matrix	348:379	the natural extracellular matrix of bone tissue	348:394	Inspired by the natural extracellular matrix of bone tissue, organic-inorganic composite scaffolds with three-dimensional (3D) porous structures, sufficient mechanical properties, excellent cytocompatibility, osteoconductivity, and osteogenic potential have received considerable attention within the field of bone engineering.
32781127	3	85	dep	epichlorohydrin	686:700	arg1	cellulose					733:741	-crosslinked hydroxyethyl cellulose	707:741	a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp)	678:864	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	3	85	dep	epichlorohydrin	686:700	arg1	EHSS					805:808	EHSS	805:808	EHSS	805:808	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	3	85	dep	epichlorohydrin	686:700	arg1	scaffold					795:802	/soy protein isolate (SPI) porous bi-component scaffold	748:802	a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp)	678:864	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	3	85	dep	epichlorohydrin	686:700	arg1	HEC					744:746	HEC	744:746	HEC	744:746	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	10	86	theme	computed	2279:2286	arg1	micro-CT					2300:2307	micro-CT	2300:2307	micro-CT	2300:2307	Microscopic computed tomography (micro-CT) results demonstrated that the bone defect site was nearly occupied with newly formed bone at 12 weeks after implantation of EHSS/HAp with 70% SPI content into the defect.
32781127	10	86	theme	computed	2279:2286	arg1	tomography					2288:2297	computed tomography	2279:2297	Microscopic computed tomography (micro-CT) results	2267:2316	Microscopic computed tomography (micro-CT) results demonstrated that the bone defect site was nearly occupied with newly formed bone at 12 weeks after implantation of EHSS/HAp with 70% SPI content into the defect.
32781127	3	87	theme	hydroxyethyl	720:731	arg1	cellulose					733:741	-crosslinked hydroxyethyl cellulose	707:741	a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp)	678:864	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	3	87	theme	hydroxyethyl	720:731	arg1	HEC					744:746	HEC	744:746	HEC	744:746	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	6	88	theme	X-ray	1580:1584	arg1	spectroscopy					1586:1597	energy dispersive X-ray spectroscopy	1562:1597	energy dispersive X-ray spectroscopy analysis	1562:1606	In particular, semiquantitative analysis showed that the calcium/phosphorus (Ca/P) ratio of EHSS/HAp with 70% SPI content (1.65) was similar to that of natural bone tissue (1.67) according to energy dispersive X-ray spectroscopy analysis.
32781127	10	89	theme	tomography	2288:2297	arg1	results					2310:2316	Microscopic computed tomography (micro-CT) results	2267:2316	Microscopic computed tomography (micro-CT) results	2267:2316	Microscopic computed tomography (micro-CT) results demonstrated that the bone defect site was nearly occupied with newly formed bone at 12 weeks after implantation of EHSS/HAp with 70% SPI content into the defect.
32781127	3	90	theme	protein	753:759	arg1	EHSS					805:808	EHSS	805:808	EHSS	805:808	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	3	90	theme	protein	753:759	arg1	scaffold					795:802	/soy protein isolate (SPI) porous bi-component scaffold	748:802	a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp)	678:864	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	6	91	theme	energy	1562:1567	arg1	spectroscopy					1586:1597	energy dispersive X-ray spectroscopy	1562:1597	energy dispersive X-ray spectroscopy analysis	1562:1606	In particular, semiquantitative analysis showed that the calcium/phosphorus (Ca/P) ratio of EHSS/HAp with 70% SPI content (1.65) was similar to that of natural bone tissue (1.67) according to energy dispersive X-ray spectroscopy analysis.
32781127	6	92	theme	EHSS/HAp	1462:1469	arg1	similar					1503:1509	similar	1503:1509	similar	1503:1509	In particular, semiquantitative analysis showed that the calcium/phosphorus (Ca/P) ratio of EHSS/HAp with 70% SPI content (1.65) was similar to that of natural bone tissue (1.67) according to energy dispersive X-ray spectroscopy analysis.
32781127	6	92	theme	EHSS/HAp	1462:1469	arg1	ratio					1453:1457	the calcium/phosphorus (Ca/P) ratio	1423:1457	the calcium/phosphorus (Ca/P) ratio of EHSS/HAp with 70% SPI content (1.65)	1423:1497	In particular, semiquantitative analysis showed that the calcium/phosphorus (Ca/P) ratio of EHSS/HAp with 70% SPI content (1.65) was similar to that of natural bone tissue (1.67) according to energy dispersive X-ray spectroscopy analysis.
32781127	10	93	theme	at	2400:2401	arg1	12 weeks					2403:2410	newly formed bone at 12 weeks	2382:2410	newly formed bone at 12 weeks after implantation of EHSS/HAp with 70% SPI content into the defect	2382:2478	Microscopic computed tomography (micro-CT) results demonstrated that the bone defect site was nearly occupied with newly formed bone at 12 weeks after implantation of EHSS/HAp with 70% SPI content into the defect.
32781127	10	94	theme	bone	2340:2343	arg1	site					2352:2355	the bone defect site	2336:2355	the bone defect site	2336:2355	Microscopic computed tomography (micro-CT) results demonstrated that the bone defect site was nearly occupied with newly formed bone at 12 weeks after implantation of EHSS/HAp with 70% SPI content into the defect.
32781127	5	95	from	properties	1307:1316	arg1	rate					1364:1367	a controllable degradation rate	1337:1367	a controllable degradation rate	1337:1367	The results indicated that the prepared scaffolds exhibited an interconnected porous structure, a biomimetic HAp coating on their surfaces, improved mechanical properties in compression and a controllable degradation rate.
32781127	5	95	from	properties	1307:1316	arg1	compression					1321:1331	compression	1321:1331	compression	1321:1331	The results indicated that the prepared scaffolds exhibited an interconnected porous structure, a biomimetic HAp coating on their surfaces, improved mechanical properties in compression and a controllable degradation rate.
32781127	6	96	theme	Ca/P	1447:1450	arg1	similar					1503:1509	similar	1503:1509	similar	1503:1509	In particular, semiquantitative analysis showed that the calcium/phosphorus (Ca/P) ratio of EHSS/HAp with 70% SPI content (1.65) was similar to that of natural bone tissue (1.67) according to energy dispersive X-ray spectroscopy analysis.
32781127	6	96	theme	Ca/P	1447:1450	arg1	ratio					1453:1457	the calcium/phosphorus (Ca/P) ratio	1423:1457	the calcium/phosphorus (Ca/P) ratio of EHSS/HAp with 70% SPI content (1.65)	1423:1497	In particular, semiquantitative analysis showed that the calcium/phosphorus (Ca/P) ratio of EHSS/HAp with 70% SPI content (1.65) was similar to that of natural bone tissue (1.67) according to energy dispersive X-ray spectroscopy analysis.
32781127	8	97	theme	genes	1967:1971	arg1	expression					1932:1941	the expression	1928:1941	the expression of osteogenesis-related genes (Col-1, Runx2, OPN, and OCN) during osteogenic differentiation of MC3T3-E1 cells	1928:2052	Consistently, in vitro osteogenic differentiation studies showed that EHSS/HAp with 70% SPI content can significantly accelerate the expression of osteogenesis-related genes (Col-1, Runx2, OPN, and OCN) during osteogenic differentiation of MC3T3-E1 cells.
32781127	1	98	theme	bone	197:200	arg1	regeneration					202:213	bone regeneration	197:213	bone regeneration	197:213	Although various strategies have been utilized to accelerate bone regeneration in bone tissue engineering (BTE), the treatment and repair of large bone defects remains a clinical challenge worldwide.
32781127	7	99	theme	70	1676:1677	arg1	%					1678:1678	%	1678:1678	%	1678:1678	In vitro cell culture experiments indicated that the EHSS/HAp with 70% SPI content showed improved cytocompatibility and was suitable for MC3T3-E1 cell attachment, proliferation and growth.
32781127	2	100	theme	engineering	651:661	arg1	field					637:641	the field	633:641	the field of bone engineering	633:661	Inspired by the natural extracellular matrix of bone tissue, organic-inorganic composite scaffolds with three-dimensional (3D) porous structures, sufficient mechanical properties, excellent cytocompatibility, osteoconductivity, and osteogenic potential have received considerable attention within the field of bone engineering.
32781127	3	101	link	-crosslinked	707:718	arg1	cellulose					733:741	-crosslinked hydroxyethyl cellulose	707:741	a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp)	678:864	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	3	101	link	-crosslinked	707:718	arg1	HEC					744:746	HEC	744:746	HEC	744:746	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	12	102	theme	%	2754:2754	arg1	SPI					2756:2758	70% SPI	2752:2758	70% SPI	2752:2758	Collectively, our results demonstrate the potential of EHSS/HAp scaffolds with 70% SPI for successful bone defect repair and regeneration.
32781127	5	103	from	coating	1260:1266	arg1	surfaces					1277:1284	their surfaces	1271:1284	their surfaces	1271:1284	The results indicated that the prepared scaffolds exhibited an interconnected porous structure, a biomimetic HAp coating on their surfaces, improved mechanical properties in compression and a controllable degradation rate.
32781127	10	104	from	12 weeks	2403:2410	arg1	at					2400:2401	newly formed bone at 12 weeks	2382:2410	newly formed bone at 12 weeks after implantation of EHSS/HAp with 70% SPI content into the defect	2382:2478	Microscopic computed tomography (micro-CT) results demonstrated that the bone defect site was nearly occupied with newly formed bone at 12 weeks after implantation of EHSS/HAp with 70% SPI content into the defect.
32781127	10	104	from	12 weeks	2403:2410	arg1	bone					2395:2398	newly formed bone at 12 weeks	2382:2410	newly formed bone at 12 weeks after implantation of EHSS/HAp with 70% SPI content into the defect	2382:2478	Microscopic computed tomography (micro-CT) results demonstrated that the bone defect site was nearly occupied with newly formed bone at 12 weeks after implantation of EHSS/HAp with 70% SPI content into the defect.
32781127	9	105	theme	tissue	2206:2211	arg1	regeneration					2213:2224	tissue regeneration	2206:2224	tissue regeneration	2206:2224	Furthermore, when applied to the repair of critical-sized cranial defects in a rat model, EHSS/HAp with 70% SPI was capable of significantly promoting tissue regeneration and integration with native bone tissue.
32781127	6	106	theme	semiquantitative	1385:1400	arg1	analysis					1402:1409	semiquantitative analysis	1385:1409	semiquantitative analysis	1385:1409	In particular, semiquantitative analysis showed that the calcium/phosphorus (Ca/P) ratio of EHSS/HAp with 70% SPI content (1.65) was similar to that of natural bone tissue (1.67) according to energy dispersive X-ray spectroscopy analysis.
32781127	1	107	theme	tissue	223:228	arg1	BTE					243:245	BTE	243:245	BTE	243:245	Although various strategies have been utilized to accelerate bone regeneration in bone tissue engineering (BTE), the treatment and repair of large bone defects remains a clinical challenge worldwide.
32781127	1	107	theme	tissue	223:228	arg1	engineering					230:240	bone tissue engineering	218:240	bone tissue engineering (BTE)	218:246	Although various strategies have been utilized to accelerate bone regeneration in bone tissue engineering (BTE), the treatment and repair of large bone defects remains a clinical challenge worldwide.
32781127	12	108	theme	EHSS/HAp	2728:2735	arg1	scaffolds					2737:2745	EHSS/HAp scaffolds	2728:2745	EHSS/HAp scaffolds	2728:2745	Collectively, our results demonstrate the potential of EHSS/HAp scaffolds with 70% SPI for successful bone defect repair and regeneration.
32781127	1	109	theme	challenge	315:323	arg1	worldwide					325:333	a clinical challenge worldwide	304:333	a clinical challenge worldwide	304:333	Although various strategies have been utilized to accelerate bone regeneration in bone tissue engineering (BTE), the treatment and repair of large bone defects remains a clinical challenge worldwide.
32781127	10	110	theme	formed	2388:2393	arg1	12 weeks					2403:2410	newly formed bone at 12 weeks	2382:2410	newly formed bone at 12 weeks after implantation of EHSS/HAp with 70% SPI content into the defect	2382:2478	Microscopic computed tomography (micro-CT) results demonstrated that the bone defect site was nearly occupied with newly formed bone at 12 weeks after implantation of EHSS/HAp with 70% SPI content into the defect.
32781127	10	111	with	EHSS/HAp	2434:2441	arg1	content					2456:2462	70% SPI content	2448:2462	70% SPI content into the defect	2448:2478	Microscopic computed tomography (micro-CT) results demonstrated that the bone defect site was nearly occupied with newly formed bone at 12 weeks after implantation of EHSS/HAp with 70% SPI content into the defect.
32781127	3	112	theme	bi-component	782:793	arg1	EHSS					805:808	EHSS	805:808	EHSS	805:808	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	3	112	theme	bi-component	782:793	arg1	scaffold					795:802	/soy protein isolate (SPI) porous bi-component scaffold	748:802	a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp)	678:864	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	7	113	theme	cell	1618:1621	arg1	experiments					1631:1641	In vitro cell culture experiments	1609:1641	In vitro cell culture experiments	1609:1641	In vitro cell culture experiments indicated that the EHSS/HAp with 70% SPI content showed improved cytocompatibility and was suitable for MC3T3-E1 cell attachment, proliferation and growth.
32781127	11	114	theme	histological	2553:2564	arg1	sections					2566:2573	histological sections	2553:2573	histological sections	2553:2573	Hematoxylin and eosin (H&E) staining and Masson's trichrome staining of histological sections further confirmed that EHSS/HAp with 70% SPI markedly promoted new bone formation and maturation.
32781127	5	115	theme	controllable	1339:1350	arg1	rate					1364:1367	a controllable degradation rate	1337:1367	a controllable degradation rate	1337:1367	The results indicated that the prepared scaffolds exhibited an interconnected porous structure, a biomimetic HAp coating on their surfaces, improved mechanical properties in compression and a controllable degradation rate.
32781127	3	116	theme	hydroxyapatite	816:829	arg1	EHSS/HAp					856:863	EHSS/HAp	856:863	EHSS/HAp	856:863	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	3	116	theme	hydroxyapatite	816:829	arg1	functionalization					837:853	hydroxyapatite (HAp) functionalization	816:853	hydroxyapatite (HAp) functionalization (EHSS/HAp)	816:864	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	7	117	theme	In	1609:1610	arg1	experiments					1631:1641	In vitro cell culture experiments	1609:1641	In vitro cell culture experiments	1609:1641	In vitro cell culture experiments indicated that the EHSS/HAp with 70% SPI content showed improved cytocompatibility and was suitable for MC3T3-E1 cell attachment, proliferation and growth.
32781127	4	118	theme	Systematic	982:991	arg1	experiments					1010:1020	Systematic characterization experiments	982:1020	Systematic characterization experiments	982:1020	Systematic characterization experiments were performed to assess the morphology, HAp-forming properties, mechanical properties and degradation rate of the scaffold.
32781127	5	119	theme	mechanical	1296:1305	arg1	structure					1232:1240	an interconnected porous structure	1207:1240	an interconnected porous structure	1207:1240	The results indicated that the prepared scaffolds exhibited an interconnected porous structure, a biomimetic HAp coating on their surfaces, improved mechanical properties in compression and a controllable degradation rate.
32781127	5	119	theme	mechanical	1296:1305	arg1	properties					1307:1316	improved mechanical properties	1287:1316	improved mechanical properties in compression and a controllable degradation rate	1287:1367	The results indicated that the prepared scaffolds exhibited an interconnected porous structure, a biomimetic HAp coating on their surfaces, improved mechanical properties in compression and a controllable degradation rate.
32781127	9	120	from	defects	2121:2127	arg1	model					2138:2142	a rat model	2132:2142	a rat model	2132:2142	Furthermore, when applied to the repair of critical-sized cranial defects in a rat model, EHSS/HAp with 70% SPI was capable of significantly promoting tissue regeneration and integration with native bone tissue.
32781127	8	121	theme	osteogenic	2009:2018	arg1	differentiation					2020:2034	osteogenic differentiation	2009:2034	osteogenic differentiation of MC3T3-E1 cells	2009:2052	Consistently, in vitro osteogenic differentiation studies showed that EHSS/HAp with 70% SPI content can significantly accelerate the expression of osteogenesis-related genes (Col-1, Runx2, OPN, and OCN) during osteogenic differentiation of MC3T3-E1 cells.
32781127	3	122	dep	in	947:948	arg1	situ					950:953	situ	950:953	situ	950:953	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	3	123	theme	defect	891:896	arg1	repair					898:903	bone defect repair	886:903	bone defect repair	886:903	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	2	124	theme	mechanical	493:502	arg1	properties					504:513	sufficient mechanical properties	482:513	sufficient mechanical properties	482:513	Inspired by the natural extracellular matrix of bone tissue, organic-inorganic composite scaffolds with three-dimensional (3D) porous structures, sufficient mechanical properties, excellent cytocompatibility, osteoconductivity, and osteogenic potential have received considerable attention within the field of bone engineering.
32781127	9	125	with	integration	2230:2240	arg1	tissue					2259:2264	native bone tissue	2247:2264	native bone tissue	2247:2264	Furthermore, when applied to the repair of critical-sized cranial defects in a rat model, EHSS/HAp with 70% SPI was capable of significantly promoting tissue regeneration and integration with native bone tissue.
32781127	5	126	theme	prepared	1178:1185	arg1	scaffolds					1187:1195	the prepared scaffolds	1174:1195	the prepared scaffolds	1174:1195	The results indicated that the prepared scaffolds exhibited an interconnected porous structure, a biomimetic HAp coating on their surfaces, improved mechanical properties in compression and a controllable degradation rate.
32781127	7	127	theme	MC3T3-E1	1747:1754	arg1	cell					1756:1759	MC3T3-E1 cell attachment, proliferation and growth	1747:1796	cell	1756:1759	In vitro cell culture experiments indicated that the EHSS/HAp with 70% SPI content showed improved cytocompatibility and was suitable for MC3T3-E1 cell attachment, proliferation and growth.
32781127	9	128	from	model	2138:2142	arg1	repair					2088:2093	the repair	2084:2093	the repair of critical-sized cranial defects in a rat model	2084:2142	Furthermore, when applied to the repair of critical-sized cranial defects in a rat model, EHSS/HAp with 70% SPI was capable of significantly promoting tissue regeneration and integration with native bone tissue.
32781127	9	129	with	regeneration	2213:2224	arg1	tissue					2259:2264	native bone tissue	2247:2264	native bone tissue	2247:2264	Furthermore, when applied to the repair of critical-sized cranial defects in a rat model, EHSS/HAp with 70% SPI was capable of significantly promoting tissue regeneration and integration with native bone tissue.
32781127	3	130	theme	HAp	832:834	arg1	EHSS/HAp					856:863	EHSS/HAp	856:863	EHSS/HAp	856:863	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	3	130	theme	HAp	832:834	arg1	functionalization					837:853	hydroxyapatite (HAp) functionalization	816:853	hydroxyapatite (HAp) functionalization (EHSS/HAp)	816:864	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	8	131	theme	cells	2048:2052	arg1	differentiation					2020:2034	osteogenic differentiation	2009:2034	osteogenic differentiation of MC3T3-E1 cells	2009:2052	Consistently, in vitro osteogenic differentiation studies showed that EHSS/HAp with 70% SPI content can significantly accelerate the expression of osteogenesis-related genes (Col-1, Runx2, OPN, and OCN) during osteogenic differentiation of MC3T3-E1 cells.
32781127	2	132	theme	tissue	389:394	arg1	matrix					374:379	the natural extracellular matrix	348:379	the natural extracellular matrix of bone tissue	348:394	Inspired by the natural extracellular matrix of bone tissue, organic-inorganic composite scaffolds with three-dimensional (3D) porous structures, sufficient mechanical properties, excellent cytocompatibility, osteoconductivity, and osteogenic potential have received considerable attention within the field of bone engineering.
32781127	6	133	with	EHSS/HAp	1462:1469	arg1	content					1484:1490	70% SPI content	1476:1490	70% SPI content	1476:1490	In particular, semiquantitative analysis showed that the calcium/phosphorus (Ca/P) ratio of EHSS/HAp with 70% SPI content (1.65) was similar to that of natural bone tissue (1.67) according to energy dispersive X-ray spectroscopy analysis.
32781127	10	134	theme	%	2450:2450	arg1	content					2456:2462	70% SPI content	2448:2462	70% SPI content into the defect	2448:2478	Microscopic computed tomography (micro-CT) results demonstrated that the bone defect site was nearly occupied with newly formed bone at 12 weeks after implantation of EHSS/HAp with 70% SPI content into the defect.
32781127	12	135	theme	scaffolds	2737:2745	arg1	potential					2715:2723	the potential	2711:2723	the potential of EHSS/HAp scaffolds with 70% SPI for successful bone defect repair and regeneration	2711:2809	Collectively, our results demonstrate the potential of EHSS/HAp scaffolds with 70% SPI for successful bone defect repair and regeneration.
32781127	7	136	dep	cell	1756:1759	arg1	attachment					1761:1770	attachment	1761:1770	attachment	1761:1770	In vitro cell culture experiments indicated that the EHSS/HAp with 70% SPI content showed improved cytocompatibility and was suitable for MC3T3-E1 cell attachment, proliferation and growth.
32781127	0	137	theme	novel	29:33	arg1	scaffolds					78:86	novel hydroxyethyl cellulose/soy protein isolate scaffolds	29:86	novel hydroxyethyl cellulose/soy protein isolate scaffolds	29:86	Biomimetic mineralization of novel hydroxyethyl cellulose/soy protein isolate scaffolds promote bone regeneration in vitro and in vivo.
32781127	1	138	theme	large	277:281	arg1	defects					288:294	large bone defects	277:294	large bone defects	277:294	Although various strategies have been utilized to accelerate bone regeneration in bone tissue engineering (BTE), the treatment and repair of large bone defects remains a clinical challenge worldwide.
32781127	8	139	theme	SPI	1887:1889	arg1	content					1891:1897	70% SPI content	1883:1897	70% SPI content	1883:1897	Consistently, in vitro osteogenic differentiation studies showed that EHSS/HAp with 70% SPI content can significantly accelerate the expression of osteogenesis-related genes (Col-1, Runx2, OPN, and OCN) during osteogenic differentiation of MC3T3-E1 cells.
32781127	3	140	theme	in	947:948	arg1	mineralization					966:979	in situ biomimetic mineralization	947:979	in situ biomimetic mineralization	947:979	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	2	141	theme	three-dimensional	440:456	arg1	structures					470:479	three-dimensional (3D) porous structures	440:479	three-dimensional (3D) porous structures	440:479	Inspired by the natural extracellular matrix of bone tissue, organic-inorganic composite scaffolds with three-dimensional (3D) porous structures, sufficient mechanical properties, excellent cytocompatibility, osteoconductivity, and osteogenic potential have received considerable attention within the field of bone engineering.
32781127	11	142	theme	new	2638:2640	arg1	formation					2647:2655	new bone formation	2638:2655	new bone formation	2638:2655	Hematoxylin and eosin (H&E) staining and Masson's trichrome staining of histological sections further confirmed that EHSS/HAp with 70% SPI markedly promoted new bone formation and maturation.
32781127	6	143	theme	%	1478:1478	arg1	content					1484:1490	70% SPI content	1476:1490	70% SPI content	1476:1490	In particular, semiquantitative analysis showed that the calcium/phosphorus (Ca/P) ratio of EHSS/HAp with 70% SPI content (1.65) was similar to that of natural bone tissue (1.67) according to energy dispersive X-ray spectroscopy analysis.
32781127	1	144	theme	defects	288:294	arg1	treatment					253:261	treatment	253:261	treatment	253:261	Although various strategies have been utilized to accelerate bone regeneration in bone tissue engineering (BTE), the treatment and repair of large bone defects remains a clinical challenge worldwide.
32781127	1	144	theme	defects	288:294	arg1	repair					267:272	repair	267:272	repair	267:272	Although various strategies have been utilized to accelerate bone regeneration in bone tissue engineering (BTE), the treatment and repair of large bone defects remains a clinical challenge worldwide.
32781127	3	145	theme	lyophilization	928:941	arg1	combination					913:923	the combination	909:923	the combination of lyophilization and in situ biomimetic mineralization	909:979	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	9	146	theme	cranial	2113:2119	arg1	defects					2121:2127	critical-sized cranial defects	2098:2127	critical-sized cranial defects in a rat model	2098:2142	Furthermore, when applied to the repair of critical-sized cranial defects in a rat model, EHSS/HAp with 70% SPI was capable of significantly promoting tissue regeneration and integration with native bone tissue.
32781127	2	147	theme	organic-inorganic	397:413	arg1	scaffolds					425:433	organic-inorganic composite scaffolds	397:433	organic-inorganic composite scaffolds with three-dimensional (3D) porous structures, sufficient mechanical properties, excellent cytocompatibility, osteoconductivity, and osteogenic potential	397:587	Inspired by the natural extracellular matrix of bone tissue, organic-inorganic composite scaffolds with three-dimensional (3D) porous structures, sufficient mechanical properties, excellent cytocompatibility, osteoconductivity, and osteogenic potential have received considerable attention within the field of bone engineering.
32781127	0	148	theme	isolate	70:76	arg1	scaffolds					78:86	novel hydroxyethyl cellulose/soy protein isolate scaffolds	29:86	novel hydroxyethyl cellulose/soy protein isolate scaffolds	29:86	Biomimetic mineralization of novel hydroxyethyl cellulose/soy protein isolate scaffolds promote bone regeneration in vitro and in vivo.
32781127	2	149	theme	porous	463:468	arg1	structures					470:479	three-dimensional (3D) porous structures	440:479	three-dimensional (3D) porous structures	440:479	Inspired by the natural extracellular matrix of bone tissue, organic-inorganic composite scaffolds with three-dimensional (3D) porous structures, sufficient mechanical properties, excellent cytocompatibility, osteoconductivity, and osteogenic potential have received considerable attention within the field of bone engineering.
32781127	2	150	dep	three-dimensional	440:456	arg1	3D					459:460	3D	459:460	3D	459:460	Inspired by the natural extracellular matrix of bone tissue, organic-inorganic composite scaffolds with three-dimensional (3D) porous structures, sufficient mechanical properties, excellent cytocompatibility, osteoconductivity, and osteogenic potential have received considerable attention within the field of bone engineering.
32781127	6	151	theme	bone	1530:1533	arg1	1.67					1543:1546	1.67	1543:1546	1.67	1543:1546	In particular, semiquantitative analysis showed that the calcium/phosphorus (Ca/P) ratio of EHSS/HAp with 70% SPI content (1.65) was similar to that of natural bone tissue (1.67) according to energy dispersive X-ray spectroscopy analysis.
32781127	6	151	theme	bone	1530:1533	arg1	tissue					1535:1540	natural bone tissue	1522:1540	natural bone tissue (1.67) according to energy dispersive X-ray spectroscopy analysis	1522:1606	In particular, semiquantitative analysis showed that the calcium/phosphorus (Ca/P) ratio of EHSS/HAp with 70% SPI content (1.65) was similar to that of natural bone tissue (1.67) according to energy dispersive X-ray spectroscopy analysis.
32781127	5	152	theme	interconnected	1210:1223	arg1	coating					1260:1266	a biomimetic HAp coating	1243:1266	a biomimetic HAp coating on their surfaces	1243:1284	The results indicated that the prepared scaffolds exhibited an interconnected porous structure, a biomimetic HAp coating on their surfaces, improved mechanical properties in compression and a controllable degradation rate.
32781127	5	152	theme	interconnected	1210:1223	arg1	structure					1232:1240	an interconnected porous structure	1207:1240	an interconnected porous structure	1207:1240	The results indicated that the prepared scaffolds exhibited an interconnected porous structure, a biomimetic HAp coating on their surfaces, improved mechanical properties in compression and a controllable degradation rate.
32781127	5	152	theme	interconnected	1210:1223	arg1	properties					1307:1316	improved mechanical properties	1287:1316	improved mechanical properties in compression and a controllable degradation rate	1287:1367	The results indicated that the prepared scaffolds exhibited an interconnected porous structure, a biomimetic HAp coating on their surfaces, improved mechanical properties in compression and a controllable degradation rate.
32781127	8	153	theme	%	1885:1885	arg1	content					1891:1897	70% SPI content	1883:1897	70% SPI content	1883:1897	Consistently, in vitro osteogenic differentiation studies showed that EHSS/HAp with 70% SPI content can significantly accelerate the expression of osteogenesis-related genes (Col-1, Runx2, OPN, and OCN) during osteogenic differentiation of MC3T3-E1 cells.
32781127	3	154	theme	biomimetic	955:964	arg1	mineralization					966:979	in situ biomimetic mineralization	947:979	in situ biomimetic mineralization	947:979	In this work, a novel epichlorohydrin (ECH)-crosslinked hydroxyethyl cellulose (HEC)/soy protein isolate (SPI) porous bi-component scaffold (EHSS) with hydroxyapatite (HAp) functionalization (EHSS/HAp) was constructed for bone defect repair via the combination of lyophilization and in situ biomimetic mineralization.
32781127	4	155	theme	degradation	1113:1123	arg1	rate					1125:1128	degradation rate	1113:1128	degradation rate	1113:1128	Systematic characterization experiments were performed to assess the morphology, HAp-forming properties, mechanical properties and degradation rate of the scaffold.
32781127	9	156	theme	rat	2134:2136	arg1	model					2138:2142	a rat model	2132:2142	a rat model	2132:2142	Furthermore, when applied to the repair of critical-sized cranial defects in a rat model, EHSS/HAp with 70% SPI was capable of significantly promoting tissue regeneration and integration with native bone tissue.
32781127	8	157	dep	in	1813:1814	arg1	vitro					1816:1820	vitro	1816:1820	vitro	1816:1820	Consistently, in vitro osteogenic differentiation studies showed that EHSS/HAp with 70% SPI content can significantly accelerate the expression of osteogenesis-related genes (Col-1, Runx2, OPN, and OCN) during osteogenic differentiation of MC3T3-E1 cells.
32781127	8	158	with	EHSS/HAp	1869:1876	arg1	content					1891:1897	70% SPI content	1883:1897	70% SPI content	1883:1897	Consistently, in vitro osteogenic differentiation studies showed that EHSS/HAp with 70% SPI content can significantly accelerate the expression of osteogenesis-related genes (Col-1, Runx2, OPN, and OCN) during osteogenic differentiation of MC3T3-E1 cells.
32781127	2	159	theme	extracellular	360:372	arg1	matrix					374:379	the natural extracellular matrix	348:379	the natural extracellular matrix of bone tissue	348:394	Inspired by the natural extracellular matrix of bone tissue, organic-inorganic composite scaffolds with three-dimensional (3D) porous structures, sufficient mechanical properties, excellent cytocompatibility, osteoconductivity, and osteogenic potential have received considerable attention within the field of bone engineering.
32781127	5	160	theme	improved	1287:1294	arg1	structure					1232:1240	an interconnected porous structure	1207:1240	an interconnected porous structure	1207:1240	The results indicated that the prepared scaffolds exhibited an interconnected porous structure, a biomimetic HAp coating on their surfaces, improved mechanical properties in compression and a controllable degradation rate.
32781127	5	160	theme	improved	1287:1294	arg1	properties					1307:1316	improved mechanical properties	1287:1316	improved mechanical properties in compression and a controllable degradation rate	1287:1367	The results indicated that the prepared scaffolds exhibited an interconnected porous structure, a biomimetic HAp coating on their surfaces, improved mechanical properties in compression and a controllable degradation rate.
33354968	6	0	theme	key	1127:1129	arg1	pathways					1142:1149	key osteogenic pathways	1127:1149	key osteogenic pathways	1127:1149	The intracellular and extracellular Zn2+ released from nano ZIF-8 and the receptors involved in the endocytosis may play a role in inducing activation of key osteogenic pathways.
33354968	8	1	theme	bone	1620:1623	arg1	engineering					1632:1642	bone tissue engineering	1620:1642	bone tissue engineering	1620:1642	Taken together, this study helps to elucidate the mechanism by which nano ZIF-8 regulates osteogenesis and suggests it to be a potential biomaterial for constructing multifunctional composites in bone tissue engineering.
33354968	6	2	theme	nano	1028:1031	arg1	ZIF-8					1033:1037	nano ZIF-8	1028:1037	nano ZIF-8	1028:1037	The intracellular and extracellular Zn2+ released from nano ZIF-8 and the receptors involved in the endocytosis may play a role in inducing activation of key osteogenic pathways.
33354968	0	3	theme	Bone	85:88	arg1	Repair					90:95	Bone Repair	85:95	Bone Repair	85:95	Nanoscale Zeolitic Imidazolate Framework-8 Activator of Canonical MAPK Signaling for Bone Repair.
33354968	3	4	theme	osteogenic	672:681	arg1	effect					683:688	a better osteogenic effect	663:688	a better osteogenic effect	663:688	Presented here is a nanoscale ZIF-8 that could drive rat bone mesenchymal stem cell (rBMSC) differentiation into osteoblasts both in vitro and in vivo, and interestingly, nano ZIF-8 exhibited a better osteogenic effect compared with ionic conditions of Zn at the same concentration of Zn2+.
33354968	5	5	theme	rBMSC	892:896	arg1	cytoplasm					898:906	the rBMSC cytoplasm	888:906	the rBMSC cytoplasm	888:906	Specifically, nano ZIF-8 could enter the rBMSC cytoplasm probably via caveolae-mediated endocytosis and macropinocytosis.
33354968	7	6	dep	phosphorylated	1291:1304	arg1	promoting					1386:1394	promoting	1386:1394	promoting the osteogenesis of rBMSCs	1386:1421	Furthermore, through transcriptome sequencing, multiple osteogenic pathways were found to be upregulated, among which nano ZIF-8 primarily phosphorylated ERK, thus activating the canonical mitogen-activated protein kinase pathway and promoting the osteogenesis of rBMSCs.
33354968	7	6	dep	phosphorylated	1291:1304	arg1	activating					1316:1325	activating	1316:1325	activating the canonical mitogen-activated protein kinase pathway	1316:1380	Furthermore, through transcriptome sequencing, multiple osteogenic pathways were found to be upregulated, among which nano ZIF-8 primarily phosphorylated ERK, thus activating the canonical mitogen-activated protein kinase pathway and promoting the osteogenesis of rBMSCs.
33354968	8	7	theme	potential	1551:1559	arg1	biomaterial					1561:1571	a potential biomaterial	1549:1571	a potential biomaterial for constructing multifunctional composites in bone tissue engineering	1549:1642	Taken together, this study helps to elucidate the mechanism by which nano ZIF-8 regulates osteogenesis and suggests it to be a potential biomaterial for constructing multifunctional composites in bone tissue engineering.
33354968	8	7	theme	potential	1551:1559	arg1	it					1540:1541	it	1540:1541	it	1540:1541	Taken together, this study helps to elucidate the mechanism by which nano ZIF-8 regulates osteogenesis and suggests it to be a potential biomaterial for constructing multifunctional composites in bone tissue engineering.
33354968	7	8	theme	rBMSCs	1416:1421	arg1	osteogenesis					1400:1411	the osteogenesis	1396:1411	the osteogenesis of rBMSCs	1396:1421	Furthermore, through transcriptome sequencing, multiple osteogenic pathways were found to be upregulated, among which nano ZIF-8 primarily phosphorylated ERK, thus activating the canonical mitogen-activated protein kinase pathway and promoting the osteogenesis of rBMSCs.
33354968	2	9	theme	nano	292:295	arg1	implants					318:325	nano ZIF-8-based titanium implants	292:325	nano ZIF-8-based titanium implants	292:325	Our previous studies have demonstrated that nano ZIF-8-based titanium implants could promote osseointegration; however, its osteogenic capacity and the related mechanisms in bone regeneration have not been fully clarified.
33354968	7	10	theme	nano	1270:1273	arg1	ZIF-8					1275:1279	nano ZIF-8	1270:1279	nano ZIF-8	1270:1279	Furthermore, through transcriptome sequencing, multiple osteogenic pathways were found to be upregulated, among which nano ZIF-8 primarily phosphorylated ERK, thus activating the canonical mitogen-activated protein kinase pathway and promoting the osteogenesis of rBMSCs.
33354968	3	11	theme	nanoscale	491:499	arg1	ZIF-8					501:505	a nanoscale ZIF-8	489:505	a nanoscale ZIF-8 that could drive rat bone mesenchymal stem cell (rBMSC) differentiation into osteoblasts both in vitro and in vivo	489:620	Presented here is a nanoscale ZIF-8 that could drive rat bone mesenchymal stem cell (rBMSC) differentiation into osteoblasts both in vitro and in vivo, and interestingly, nano ZIF-8 exhibited a better osteogenic effect compared with ionic conditions of Zn at the same concentration of Zn2+.
33354968	3	12	theme	Zn	724:725	arg1	conditions					710:719	ionic conditions	704:719	ionic conditions of Zn at the same concentration of Zn2+	704:759	Presented here is a nanoscale ZIF-8 that could drive rat bone mesenchymal stem cell (rBMSC) differentiation into osteoblasts both in vitro and in vivo, and interestingly, nano ZIF-8 exhibited a better osteogenic effect compared with ionic conditions of Zn at the same concentration of Zn2+.
33354968	7	13	theme	mitogen-activated	1341:1357	arg1	kinase					1367:1372	the canonical mitogen-activated protein kinase	1327:1372	the canonical mitogen-activated protein kinase pathway	1327:1380	Furthermore, through transcriptome sequencing, multiple osteogenic pathways were found to be upregulated, among which nano ZIF-8 primarily phosphorylated ERK, thus activating the canonical mitogen-activated protein kinase pathway and promoting the osteogenesis of rBMSCs.
33354968	3	14	theme	mesenchymal	533:543	arg1	cell					550:553	rat bone mesenchymal stem cell	524:553	rat bone mesenchymal stem cell (rBMSC) differentiation into osteoblasts	524:594	Presented here is a nanoscale ZIF-8 that could drive rat bone mesenchymal stem cell (rBMSC) differentiation into osteoblasts both in vitro and in vivo, and interestingly, nano ZIF-8 exhibited a better osteogenic effect compared with ionic conditions of Zn at the same concentration of Zn2+.
33354968	3	14	theme	mesenchymal	533:543	arg1	rBMSC					556:560	rBMSC	556:560	rBMSC	556:560	Presented here is a nanoscale ZIF-8 that could drive rat bone mesenchymal stem cell (rBMSC) differentiation into osteoblasts both in vitro and in vivo, and interestingly, nano ZIF-8 exhibited a better osteogenic effect compared with ionic conditions of Zn at the same concentration of Zn2+.
33354968	5	15	theme	nano	865:868	arg1	ZIF-8					870:874	nano ZIF-8	865:874	nano ZIF-8	865:874	Specifically, nano ZIF-8 could enter the rBMSC cytoplasm probably via caveolae-mediated endocytosis and macropinocytosis.
33354968	1	16	theme	metal	163:167	arg1	framework					177:185	metal organic framework	163:185	metal organic framework	163:185	Zeolitic imidazolate framework-8 (ZIF-8) is an important type of metal organic framework and has found numerous applications in the biomedical field.
33354968	3	17	theme	ionic	704:708	arg1	conditions					710:719	ionic conditions	704:719	ionic conditions of Zn at the same concentration of Zn2+	704:759	Presented here is a nanoscale ZIF-8 that could drive rat bone mesenchymal stem cell (rBMSC) differentiation into osteoblasts both in vitro and in vivo, and interestingly, nano ZIF-8 exhibited a better osteogenic effect compared with ionic conditions of Zn at the same concentration of Zn2+.
33354968	3	18	theme	stem	545:548	arg1	cell					550:553	rat bone mesenchymal stem cell	524:553	rat bone mesenchymal stem cell (rBMSC) differentiation into osteoblasts	524:594	Presented here is a nanoscale ZIF-8 that could drive rat bone mesenchymal stem cell (rBMSC) differentiation into osteoblasts both in vitro and in vivo, and interestingly, nano ZIF-8 exhibited a better osteogenic effect compared with ionic conditions of Zn at the same concentration of Zn2+.
33354968	3	18	theme	stem	545:548	arg1	rBMSC					556:560	rBMSC	556:560	rBMSC	556:560	Presented here is a nanoscale ZIF-8 that could drive rat bone mesenchymal stem cell (rBMSC) differentiation into osteoblasts both in vitro and in vivo, and interestingly, nano ZIF-8 exhibited a better osteogenic effect compared with ionic conditions of Zn at the same concentration of Zn2+.
33354968	1	19	theme	organic	169:175	arg1	framework					177:185	metal organic framework	163:185	metal organic framework	163:185	Zeolitic imidazolate framework-8 (ZIF-8) is an important type of metal organic framework and has found numerous applications in the biomedical field.
33354968	0	20	theme	Zeolitic	10:17	arg1	Activator					43:51	Nanoscale Zeolitic Imidazolate Framework-8 Activator	0:51	Nanoscale Zeolitic Imidazolate Framework-8 Activator of Canonical MAPK Signaling for Bone Repair.	0:96	Nanoscale Zeolitic Imidazolate Framework-8 Activator of Canonical MAPK Signaling for Bone Repair.
33354968	3	21	theme	bone	528:531	arg1	cell					550:553	rat bone mesenchymal stem cell	524:553	rat bone mesenchymal stem cell (rBMSC) differentiation into osteoblasts	524:594	Presented here is a nanoscale ZIF-8 that could drive rat bone mesenchymal stem cell (rBMSC) differentiation into osteoblasts both in vitro and in vivo, and interestingly, nano ZIF-8 exhibited a better osteogenic effect compared with ionic conditions of Zn at the same concentration of Zn2+.
33354968	3	21	theme	bone	528:531	arg1	rBMSC					556:560	rBMSC	556:560	rBMSC	556:560	Presented here is a nanoscale ZIF-8 that could drive rat bone mesenchymal stem cell (rBMSC) differentiation into osteoblasts both in vitro and in vivo, and interestingly, nano ZIF-8 exhibited a better osteogenic effect compared with ionic conditions of Zn at the same concentration of Zn2+.
33354968	6	22	theme	extracellular	995:1007	arg1	Zn2+					1009:1012	The intracellular and extracellular Zn2+	973:1012	The intracellular and extracellular Zn2+ released from nano ZIF-8 and the receptors involved in the endocytosis	973:1083	The intracellular and extracellular Zn2+ released from nano ZIF-8 and the receptors involved in the endocytosis may play a role in inducing activation of key osteogenic pathways.
33354968	7	23	theme	multiple	1199:1206	arg1	pathways					1219:1226	multiple osteogenic pathways	1199:1226	multiple osteogenic pathways	1199:1226	Furthermore, through transcriptome sequencing, multiple osteogenic pathways were found to be upregulated, among which nano ZIF-8 primarily phosphorylated ERK, thus activating the canonical mitogen-activated protein kinase pathway and promoting the osteogenesis of rBMSCs.
33354968	1	24	theme	framework	177:185	arg1	type					155:158	an important type	142:158	an important type of metal organic framework	142:185	Zeolitic imidazolate framework-8 (ZIF-8) is an important type of metal organic framework and has found numerous applications in the biomedical field.
33354968	1	24	theme	framework	177:185	arg1	framework-8					119:129	Zeolitic imidazolate framework-8	98:129	Zeolitic imidazolate framework-8 (ZIF-8)	98:137	Zeolitic imidazolate framework-8 (ZIF-8) is an important type of metal organic framework and has found numerous applications in the biomedical field.
33354968	0	25	theme	Nanoscale	0:8	arg1	Activator					43:51	Nanoscale Zeolitic Imidazolate Framework-8 Activator	0:51	Nanoscale Zeolitic Imidazolate Framework-8 Activator of Canonical MAPK Signaling for Bone Repair.	0:96	Nanoscale Zeolitic Imidazolate Framework-8 Activator of Canonical MAPK Signaling for Bone Repair.
33354968	3	26	theme	same	734:737	arg1	concentration					739:751	the same concentration	730:751	the same concentration of Zn2+	730:759	Presented here is a nanoscale ZIF-8 that could drive rat bone mesenchymal stem cell (rBMSC) differentiation into osteoblasts both in vitro and in vivo, and interestingly, nano ZIF-8 exhibited a better osteogenic effect compared with ionic conditions of Zn at the same concentration of Zn2+.
33354968	3	27	from	concentration	739:751	arg1	conditions					710:719	ionic conditions	704:719	ionic conditions of Zn at the same concentration of Zn2+	704:759	Presented here is a nanoscale ZIF-8 that could drive rat bone mesenchymal stem cell (rBMSC) differentiation into osteoblasts both in vitro and in vivo, and interestingly, nano ZIF-8 exhibited a better osteogenic effect compared with ionic conditions of Zn at the same concentration of Zn2+.
33354968	2	28	from	capacity	383:390	arg1	regeneration					427:438	bone regeneration	422:438	bone regeneration	422:438	Our previous studies have demonstrated that nano ZIF-8-based titanium implants could promote osseointegration; however, its osteogenic capacity and the related mechanisms in bone regeneration have not been fully clarified.
33354968	1	29	theme	Zeolitic	98:105	arg1	type					155:158	an important type	142:158	an important type of metal organic framework	142:185	Zeolitic imidazolate framework-8 (ZIF-8) is an important type of metal organic framework and has found numerous applications in the biomedical field.
33354968	1	29	theme	Zeolitic	98:105	arg1	ZIF-8					132:136	ZIF-8	132:136	ZIF-8	132:136	Zeolitic imidazolate framework-8 (ZIF-8) is an important type of metal organic framework and has found numerous applications in the biomedical field.
33354968	1	29	theme	Zeolitic	98:105	arg1	framework-8					119:129	Zeolitic imidazolate framework-8	98:129	Zeolitic imidazolate framework-8 (ZIF-8)	98:137	Zeolitic imidazolate framework-8 (ZIF-8) is an important type of metal organic framework and has found numerous applications in the biomedical field.
33354968	3	30	theme	nano	642:645	arg1	ZIF-8					647:651	nano ZIF-8	642:651	nano ZIF-8	642:651	Presented here is a nanoscale ZIF-8 that could drive rat bone mesenchymal stem cell (rBMSC) differentiation into osteoblasts both in vitro and in vivo, and interestingly, nano ZIF-8 exhibited a better osteogenic effect compared with ionic conditions of Zn at the same concentration of Zn2+.
33354968	0	31	theme	Framework-8	31:41	arg1	Activator					43:51	Nanoscale Zeolitic Imidazolate Framework-8 Activator	0:51	Nanoscale Zeolitic Imidazolate Framework-8 Activator of Canonical MAPK Signaling for Bone Repair.	0:96	Nanoscale Zeolitic Imidazolate Framework-8 Activator of Canonical MAPK Signaling for Bone Repair.
33354968	8	32	theme	multifunctional	1590:1604	arg1	composites					1606:1615	multifunctional composites	1590:1615	multifunctional composites in bone tissue engineering	1590:1642	Taken together, this study helps to elucidate the mechanism by which nano ZIF-8 regulates osteogenesis and suggests it to be a potential biomaterial for constructing multifunctional composites in bone tissue engineering.
33354968	5	33	theme	caveolae-mediated	921:937	arg1	endocytosis					939:949	caveolae-mediated endocytosis	921:949	caveolae-mediated endocytosis	921:949	Specifically, nano ZIF-8 could enter the rBMSC cytoplasm probably via caveolae-mediated endocytosis and macropinocytosis.
33354968	1	34	theme	imidazolate	107:117	arg1	type					155:158	an important type	142:158	an important type of metal organic framework	142:185	Zeolitic imidazolate framework-8 (ZIF-8) is an important type of metal organic framework and has found numerous applications in the biomedical field.
33354968	1	34	theme	imidazolate	107:117	arg1	ZIF-8					132:136	ZIF-8	132:136	ZIF-8	132:136	Zeolitic imidazolate framework-8 (ZIF-8) is an important type of metal organic framework and has found numerous applications in the biomedical field.
33354968	1	34	theme	imidazolate	107:117	arg1	framework-8					119:129	Zeolitic imidazolate framework-8	98:129	Zeolitic imidazolate framework-8 (ZIF-8)	98:137	Zeolitic imidazolate framework-8 (ZIF-8) is an important type of metal organic framework and has found numerous applications in the biomedical field.
33354968	7	35	theme	canonical	1331:1339	arg1	kinase					1367:1372	the canonical mitogen-activated protein kinase	1327:1372	the canonical mitogen-activated protein kinase pathway	1327:1380	Furthermore, through transcriptome sequencing, multiple osteogenic pathways were found to be upregulated, among which nano ZIF-8 primarily phosphorylated ERK, thus activating the canonical mitogen-activated protein kinase pathway and promoting the osteogenesis of rBMSCs.
33354968	0	36	theme	Imidazolate	19:29	arg1	Activator					43:51	Nanoscale Zeolitic Imidazolate Framework-8 Activator	0:51	Nanoscale Zeolitic Imidazolate Framework-8 Activator of Canonical MAPK Signaling for Bone Repair.	0:96	Nanoscale Zeolitic Imidazolate Framework-8 Activator of Canonical MAPK Signaling for Bone Repair.
33354968	6	37	theme	intracellular	977:989	arg1	Zn2+					1009:1012	The intracellular and extracellular Zn2+	973:1012	The intracellular and extracellular Zn2+ released from nano ZIF-8 and the receptors involved in the endocytosis	973:1083	The intracellular and extracellular Zn2+ released from nano ZIF-8 and the receptors involved in the endocytosis may play a role in inducing activation of key osteogenic pathways.
33354968	2	38	theme	ZIF-8-based	297:307	arg1	implants					318:325	nano ZIF-8-based titanium implants	292:325	nano ZIF-8-based titanium implants	292:325	Our previous studies have demonstrated that nano ZIF-8-based titanium implants could promote osseointegration; however, its osteogenic capacity and the related mechanisms in bone regeneration have not been fully clarified.
33354968	1	39	theme	found	195:199	arg1	applications					210:221	found numerous applications	195:221	found numerous applications in the biomedical field	195:245	Zeolitic imidazolate framework-8 (ZIF-8) is an important type of metal organic framework and has found numerous applications in the biomedical field.
33354968	1	40	theme	numerous	201:208	arg1	applications					210:221	found numerous applications	195:221	found numerous applications in the biomedical field	195:245	Zeolitic imidazolate framework-8 (ZIF-8) is an important type of metal organic framework and has found numerous applications in the biomedical field.
33354968	7	41	theme	kinase	1367:1372	arg1	pathway					1374:1380	the canonical mitogen-activated protein kinase pathway	1327:1380	the canonical mitogen-activated protein kinase pathway	1327:1380	Furthermore, through transcriptome sequencing, multiple osteogenic pathways were found to be upregulated, among which nano ZIF-8 primarily phosphorylated ERK, thus activating the canonical mitogen-activated protein kinase pathway and promoting the osteogenesis of rBMSCs.
33354968	2	42	theme	bone	422:425	arg1	regeneration					427:438	bone regeneration	422:438	bone regeneration	422:438	Our previous studies have demonstrated that nano ZIF-8-based titanium implants could promote osseointegration; however, its osteogenic capacity and the related mechanisms in bone regeneration have not been fully clarified.
33354968	7	43	theme	transcriptome	1173:1185	arg1	sequencing					1187:1196	transcriptome sequencing	1173:1196	transcriptome sequencing	1173:1196	Furthermore, through transcriptome sequencing, multiple osteogenic pathways were found to be upregulated, among which nano ZIF-8 primarily phosphorylated ERK, thus activating the canonical mitogen-activated protein kinase pathway and promoting the osteogenesis of rBMSCs.
33354968	8	44	from	composites	1606:1615	arg1	engineering					1632:1642	bone tissue engineering	1620:1642	bone tissue engineering	1620:1642	Taken together, this study helps to elucidate the mechanism by which nano ZIF-8 regulates osteogenesis and suggests it to be a potential biomaterial for constructing multifunctional composites in bone tissue engineering.
33354968	0	45	theme	MAPK	66:69	arg1	Signaling					71:79	Canonical MAPK Signaling	56:79	Canonical MAPK Signaling	56:79	Nanoscale Zeolitic Imidazolate Framework-8 Activator of Canonical MAPK Signaling for Bone Repair.
33354968	8	46	theme	tissue	1625:1630	arg1	engineering					1632:1642	bone tissue engineering	1620:1642	bone tissue engineering	1620:1642	Taken together, this study helps to elucidate the mechanism by which nano ZIF-8 regulates osteogenesis and suggests it to be a potential biomaterial for constructing multifunctional composites in bone tissue engineering.
33354968	8	47	theme	nano	1493:1496	arg1	ZIF-8					1498:1502	nano ZIF-8	1493:1502	nano ZIF-8	1493:1502	Taken together, this study helps to elucidate the mechanism by which nano ZIF-8 regulates osteogenesis and suggests it to be a potential biomaterial for constructing multifunctional composites in bone tissue engineering.
33354968	4	48	theme	nanoparticles	810:822	arg1	mechanisms					792:801	the cellular uptake mechanisms	772:801	the cellular uptake mechanisms of the nanoparticles	772:822	Moreover, the cellular uptake mechanisms of the nanoparticles were thoroughly clarified.
33354968	1	49	from	applications	210:221	arg1	field					241:245	the biomedical field	226:245	the biomedical field	226:245	Zeolitic imidazolate framework-8 (ZIF-8) is an important type of metal organic framework and has found numerous applications in the biomedical field.
33354968	0	50	theme	Canonical	56:64	arg1	Signaling					71:79	Canonical MAPK Signaling	56:79	Canonical MAPK Signaling	56:79	Nanoscale Zeolitic Imidazolate Framework-8 Activator of Canonical MAPK Signaling for Bone Repair.
33354968	2	51	from	mechanisms	408:417	arg1	regeneration					427:438	bone regeneration	422:438	bone regeneration	422:438	Our previous studies have demonstrated that nano ZIF-8-based titanium implants could promote osseointegration; however, its osteogenic capacity and the related mechanisms in bone regeneration have not been fully clarified.
33354968	6	52	theme	pathways	1142:1149	arg1	activation					1113:1122	activation	1113:1122	activation of key osteogenic pathways	1113:1149	The intracellular and extracellular Zn2+ released from nano ZIF-8 and the receptors involved in the endocytosis may play a role in inducing activation of key osteogenic pathways.
33354968	3	53	theme	Zn2+	756:759	arg1	concentration					739:751	the same concentration	730:751	the same concentration of Zn2+	730:759	Presented here is a nanoscale ZIF-8 that could drive rat bone mesenchymal stem cell (rBMSC) differentiation into osteoblasts both in vitro and in vivo, and interestingly, nano ZIF-8 exhibited a better osteogenic effect compared with ionic conditions of Zn at the same concentration of Zn2+.
33354968	3	54	theme	better	665:670	arg1	effect					683:688	a better osteogenic effect	663:688	a better osteogenic effect	663:688	Presented here is a nanoscale ZIF-8 that could drive rat bone mesenchymal stem cell (rBMSC) differentiation into osteoblasts both in vitro and in vivo, and interestingly, nano ZIF-8 exhibited a better osteogenic effect compared with ionic conditions of Zn at the same concentration of Zn2+.
33354968	6	55	theme	osteogenic	1131:1140	arg1	pathways					1142:1149	key osteogenic pathways	1127:1149	key osteogenic pathways	1127:1149	The intracellular and extracellular Zn2+ released from nano ZIF-8 and the receptors involved in the endocytosis may play a role in inducing activation of key osteogenic pathways.
33354968	2	56	theme	titanium	309:316	arg1	implants					318:325	nano ZIF-8-based titanium implants	292:325	nano ZIF-8-based titanium implants	292:325	Our previous studies have demonstrated that nano ZIF-8-based titanium implants could promote osseointegration; however, its osteogenic capacity and the related mechanisms in bone regeneration have not been fully clarified.
33354968	6	57	attach	released	1014:1021	arg2	Zn2+					1009:1012	The intracellular and extracellular Zn2+	973:1012	The intracellular and extracellular Zn2+ released from nano ZIF-8 and the receptors involved in the endocytosis	973:1083	The intracellular and extracellular Zn2+ released from nano ZIF-8 and the receptors involved in the endocytosis may play a role in inducing activation of key osteogenic pathways.
33354968	6	57	attach	released	1014:1021	arg1	ZIF-8					1033:1037	nano ZIF-8	1028:1037	nano ZIF-8	1028:1037	The intracellular and extracellular Zn2+ released from nano ZIF-8 and the receptors involved in the endocytosis may play a role in inducing activation of key osteogenic pathways.
33354968	6	57	attach	released	1014:1021	arg1	receptors					1047:1055	the receptors	1043:1055	the receptors involved in the endocytosis	1043:1083	The intracellular and extracellular Zn2+ released from nano ZIF-8 and the receptors involved in the endocytosis may play a role in inducing activation of key osteogenic pathways.
33354968	2	58	theme	previous	252:259	arg1	studies					261:267	Our previous studies	248:267	Our previous studies	248:267	Our previous studies have demonstrated that nano ZIF-8-based titanium implants could promote osseointegration; however, its osteogenic capacity and the related mechanisms in bone regeneration have not been fully clarified.
33354968	2	59	theme	osteogenic	372:381	arg1	capacity					383:390	its osteogenic capacity	368:390	its osteogenic capacity	368:390	Our previous studies have demonstrated that nano ZIF-8-based titanium implants could promote osseointegration; however, its osteogenic capacity and the related mechanisms in bone regeneration have not been fully clarified.
33354968	7	60	theme	protein	1359:1365	arg1	kinase					1367:1372	the canonical mitogen-activated protein kinase	1327:1372	the canonical mitogen-activated protein kinase pathway	1327:1380	Furthermore, through transcriptome sequencing, multiple osteogenic pathways were found to be upregulated, among which nano ZIF-8 primarily phosphorylated ERK, thus activating the canonical mitogen-activated protein kinase pathway and promoting the osteogenesis of rBMSCs.
33354968	1	61	contain	has	191:193	arg2	applications					210:221	found numerous applications	195:221	found numerous applications in the biomedical field	195:245	Zeolitic imidazolate framework-8 (ZIF-8) is an important type of metal organic framework and has found numerous applications in the biomedical field.
33354968	1	61	contain	has	191:193	arg1	type					155:158	an important type	142:158	an important type of metal organic framework	142:185	Zeolitic imidazolate framework-8 (ZIF-8) is an important type of metal organic framework and has found numerous applications in the biomedical field.
33354968	1	61	contain	has	191:193	arg1	ZIF-8					132:136	ZIF-8	132:136	ZIF-8	132:136	Zeolitic imidazolate framework-8 (ZIF-8) is an important type of metal organic framework and has found numerous applications in the biomedical field.
33354968	1	61	contain	has	191:193	arg1	framework-8					119:129	Zeolitic imidazolate framework-8	98:129	Zeolitic imidazolate framework-8 (ZIF-8)	98:137	Zeolitic imidazolate framework-8 (ZIF-8) is an important type of metal organic framework and has found numerous applications in the biomedical field.
33354968	4	62	theme	uptake	785:790	arg1	mechanisms					792:801	the cellular uptake mechanisms	772:801	the cellular uptake mechanisms of the nanoparticles	772:822	Moreover, the cellular uptake mechanisms of the nanoparticles were thoroughly clarified.
33354968	3	63	theme	rat	524:526	arg1	cell					550:553	rat bone mesenchymal stem cell	524:553	rat bone mesenchymal stem cell (rBMSC) differentiation into osteoblasts	524:594	Presented here is a nanoscale ZIF-8 that could drive rat bone mesenchymal stem cell (rBMSC) differentiation into osteoblasts both in vitro and in vivo, and interestingly, nano ZIF-8 exhibited a better osteogenic effect compared with ionic conditions of Zn at the same concentration of Zn2+.
33354968	3	63	theme	rat	524:526	arg1	rBMSC					556:560	rBMSC	556:560	rBMSC	556:560	Presented here is a nanoscale ZIF-8 that could drive rat bone mesenchymal stem cell (rBMSC) differentiation into osteoblasts both in vitro and in vivo, and interestingly, nano ZIF-8 exhibited a better osteogenic effect compared with ionic conditions of Zn at the same concentration of Zn2+.
33354968	2	64	theme	related	400:406	arg1	mechanisms					408:417	the related mechanisms	396:417	the related mechanisms in bone regeneration	396:438	Our previous studies have demonstrated that nano ZIF-8-based titanium implants could promote osseointegration; however, its osteogenic capacity and the related mechanisms in bone regeneration have not been fully clarified.
33354968	1	65	theme	biomedical	230:239	arg1	field					241:245	the biomedical field	226:245	the biomedical field	226:245	Zeolitic imidazolate framework-8 (ZIF-8) is an important type of metal organic framework and has found numerous applications in the biomedical field.
33354968	0	66	theme	Signaling	71:79	arg1	Activator					43:51	Nanoscale Zeolitic Imidazolate Framework-8 Activator	0:51	Nanoscale Zeolitic Imidazolate Framework-8 Activator of Canonical MAPK Signaling for Bone Repair.	0:96	Nanoscale Zeolitic Imidazolate Framework-8 Activator of Canonical MAPK Signaling for Bone Repair.
33354968	3	67	theme	cell	550:553	arg1	differentiation					563:577	rat bone mesenchymal stem cell (rBMSC) differentiation	524:577	rat bone mesenchymal stem cell (rBMSC) differentiation into osteoblasts	524:594	Presented here is a nanoscale ZIF-8 that could drive rat bone mesenchymal stem cell (rBMSC) differentiation into osteoblasts both in vitro and in vivo, and interestingly, nano ZIF-8 exhibited a better osteogenic effect compared with ionic conditions of Zn at the same concentration of Zn2+.
33354968	4	68	theme	cellular	776:783	arg1	mechanisms					792:801	the cellular uptake mechanisms	772:801	the cellular uptake mechanisms of the nanoparticles	772:822	Moreover, the cellular uptake mechanisms of the nanoparticles were thoroughly clarified.
33354968	1	69	theme	important	145:153	arg1	type					155:158	an important type	142:158	an important type of metal organic framework	142:185	Zeolitic imidazolate framework-8 (ZIF-8) is an important type of metal organic framework and has found numerous applications in the biomedical field.
33354968	1	69	theme	important	145:153	arg1	framework-8					119:129	Zeolitic imidazolate framework-8	98:129	Zeolitic imidazolate framework-8 (ZIF-8)	98:137	Zeolitic imidazolate framework-8 (ZIF-8) is an important type of metal organic framework and has found numerous applications in the biomedical field.
33354968	7	70	theme	osteogenic	1208:1217	arg1	pathways					1219:1226	multiple osteogenic pathways	1199:1226	multiple osteogenic pathways	1199:1226	Furthermore, through transcriptome sequencing, multiple osteogenic pathways were found to be upregulated, among which nano ZIF-8 primarily phosphorylated ERK, thus activating the canonical mitogen-activated protein kinase pathway and promoting the osteogenesis of rBMSCs.
33399896	2	0	theme	new	108:110	arg1	species					128:134	A new actinobacterial species	106:134	A new actinobacterial species of the genus Myceligenerans	106:162	A new actinobacterial species of the genus Myceligenerans has been isolated from the intertidal sediment of Indian Sundarbans mangrove ecosystem.
33399896	5	1	theme	different	520:528	arg1	sources					537:543	different carbon sources	520:543	different carbon sources	520:543	The organism can grow across a wide range of temperature, salinity, and pH as well as on different carbon sources.
33399896	9	2	theme	isolate	1041:1047	arg1	content					1026:1032	The DNA G + C content	1012:1032	The DNA G + C content of the isolate	1012:1047	The DNA G + C content of the isolate has been determined as 72 mol%.
33399896	9	2	theme	isolate	1041:1047	arg1	%					1078:1078	72 mol%	1072:1078	72 mol%	1072:1078	The DNA G + C content of the isolate has been determined as 72 mol%.
33399896	14	3	theme	xiligouense	1580:1590	arg1	DSM					1599:1601	Myceligenerans xiligouense strain DSM 15,700	1565:1608	Myceligenerans xiligouense strain DSM 15,700	1565:1608	GGDC (%), orthoANIu (%), and AAI of I2 genome indicated 28.9%, 77.44% and 0.859 identity with the genome of Myceligenerans xiligouense strain DSM 15,700.
33399896	14	4	theme	Myceligenerans	1565:1578	arg1	DSM					1599:1601	Myceligenerans xiligouense strain DSM 15,700	1565:1608	Myceligenerans xiligouense strain DSM 15,700	1565:1608	GGDC (%), orthoANIu (%), and AAI of I2 genome indicated 28.9%, 77.44% and 0.859 identity with the genome of Myceligenerans xiligouense strain DSM 15,700.
33399896	11	5	theme	unknown	1281:1287	arg1	diphosphatidylglycerol					1224:1245	diphosphatidylglycerol	1224:1245	diphosphatidylglycerol	1224:1245	The polar lipids were represented by diphosphatidylglycerol, one unknown phospholipid and one unknown glycolipid.
33399896	11	5	theme	unknown	1281:1287	arg1	glycolipid					1289:1298	one unknown glycolipid	1277:1298	one unknown glycolipid	1277:1298	The polar lipids were represented by diphosphatidylglycerol, one unknown phospholipid and one unknown glycolipid.
33399896	4	6	theme	rod-shaped	410:419	arg1	elements					421:428	cocci- and rod-shaped elements	399:428	cocci- and rod-shaped elements	399:428	The isolate exhibit well-developed substrate mycelia along with the presence of cocci- and rod-shaped elements.
33399896	12	7	from	present	1319:1325	arg1	isolate					1334:1340	the isolate	1330:1340	the isolate	1330:1340	Major fatty acids present in the isolate are anteiso-C15, iso-C15, iso-C16, and anteiso-C17.
33399896	15	8	theme	Myceligenerans	1662:1675	arg1	sp					1685:1686	Myceligenerans indicum sp	1662:1686	Myceligenerans indicum sp	1662:1686	The isolate I2 has been proposed as a new species, Myceligenerans indicum sp.
33399896	15	8	theme	Myceligenerans	1662:1675	arg1	species					1653:1659	a new species	1647:1659	a new species	1647:1659	The isolate I2 has been proposed as a new species, Myceligenerans indicum sp.
33399896	7	9	theme	potential	807:815	arg1	ability					817:823	its potential ability to produce secondary metabolites	803:856	its potential ability to produce secondary metabolites	803:856	Presence of ketosynthase domain representing polyketide synthases in the isolate provides evidence of its potential ability to produce secondary metabolites.
33399896	15	10	theme	new	1649:1651	arg1	I2					1623:1624	The isolate I2	1611:1624	The isolate I2	1611:1624	The isolate I2 has been proposed as a new species, Myceligenerans indicum sp.
33399896	15	10	theme	new	1649:1651	arg1	sp					1685:1686	Myceligenerans indicum sp	1662:1686	Myceligenerans indicum sp	1662:1686	The isolate I2 has been proposed as a new species, Myceligenerans indicum sp.
33399896	15	10	theme	new	1649:1651	arg1	species					1653:1659	a new species	1647:1659	a new species	1647:1659	The isolate I2 has been proposed as a new species, Myceligenerans indicum sp.
33399896	8	11	theme	family	958:963	arg1	Promicromonosporaceae					965:985	the family Promicromonosporaceae	954:985	the family Promicromonosporaceae (Phylum Actinobacteria)	954:1009	Multigene phylogeny based on atpD and rpoB gene sequences confirmed it as a new species within the family Promicromonosporaceae (Phylum Actinobacteria).
33399896	8	11	theme	family	958:963	arg1	Actinobacteria					995:1008	Phylum Actinobacteria	988:1008	Phylum Actinobacteria	988:1008	Multigene phylogeny based on atpD and rpoB gene sequences confirmed it as a new species within the family Promicromonosporaceae (Phylum Actinobacteria).
33399896	6	12	theme	coverage	690:697	arg1	XHU					661:663	XHU	661:663	XHU	661:663	Phylogenetic analyses based on 16S rRNA showed that this isolate is closely related to Myceligenerans salitolerans XHU 5031 (99% identity; 100% coverage).
33399896	6	12	theme	coverage	690:697	arg1	%					688:688	99% identity; 100% coverage	671:697	99% identity; 100% coverage	671:697	Phylogenetic analyses based on 16S rRNA showed that this isolate is closely related to Myceligenerans salitolerans XHU 5031 (99% identity; 100% coverage).
33399896	5	13	theme	carbon	530:535	arg1	sources					537:543	different carbon sources	520:543	different carbon sources	520:543	The organism can grow across a wide range of temperature, salinity, and pH as well as on different carbon sources.
33399896	2	14	theme	genus	143:147	arg1	Myceligenerans					149:162	the genus Myceligenerans	139:162	the genus Myceligenerans	139:162	A new actinobacterial species of the genus Myceligenerans has been isolated from the intertidal sediment of Indian Sundarbans mangrove ecosystem.
33399896	8	15	theme	Phylum	988:993	arg1	Promicromonosporaceae					965:985	the family Promicromonosporaceae	954:985	the family Promicromonosporaceae (Phylum Actinobacteria)	954:1009	Multigene phylogeny based on atpD and rpoB gene sequences confirmed it as a new species within the family Promicromonosporaceae (Phylum Actinobacteria).
33399896	8	15	theme	Phylum	988:993	arg1	Actinobacteria					995:1008	Phylum Actinobacteria	988:1008	Phylum Actinobacteria	988:1008	Multigene phylogeny based on atpD and rpoB gene sequences confirmed it as a new species within the family Promicromonosporaceae (Phylum Actinobacteria).
33399896	8	16	theme	phylogeny	869:877	arg1	on atpD					885:891	Multigene phylogeny based on atpD and rpoB gene sequences	859:915	on atpD	885:891	Multigene phylogeny based on atpD and rpoB gene sequences confirmed it as a new species within the family Promicromonosporaceae (Phylum Actinobacteria).
33399896	7	17	theme	secondary	836:844	arg1	metabolites					846:856	secondary metabolites	836:856	secondary metabolites	836:856	Presence of ketosynthase domain representing polyketide synthases in the isolate provides evidence of its potential ability to produce secondary metabolites.
33399896	10	18	contain	contained	1144:1152	arg2	glucose					1154:1160	glucose	1154:1160	glucose	1154:1160	The peptidoglycan type was A4α and the whole-cell hydrolysates contained glucose, galactose, and mannose.
33399896	10	18	contain	contained	1144:1152	arg1	hydrolysates					1131:1142	the whole-cell hydrolysates	1116:1142	the whole-cell hydrolysates	1116:1142	The peptidoglycan type was A4α and the whole-cell hydrolysates contained glucose, galactose, and mannose.
33399896	10	18	contain	contained	1144:1152	arg2	mannose					1178:1184	mannose	1178:1184	mannose	1178:1184	The peptidoglycan type was A4α and the whole-cell hydrolysates contained glucose, galactose, and mannose.
33399896	10	18	contain	contained	1144:1152	arg2	galactose					1163:1171	galactose	1163:1171	galactose	1163:1171	The peptidoglycan type was A4α and the whole-cell hydrolysates contained glucose, galactose, and mannose.
33399896	4	19	theme	elements	421:428	arg1	presence					387:394	the presence	383:394	the presence of cocci- and rod-shaped elements	383:428	The isolate exhibit well-developed substrate mycelia along with the presence of cocci- and rod-shaped elements.
33399896	1	20	theme	mangrove	66:73	arg1	sediment					75:82	mangrove sediment	66:82	mangrove sediment of Sundarbans, India	66:103	nov., an actinobacterium isolated from mangrove sediment of Sundarbans, India.
33399896	8	21	theme	Multigene	859:867	arg1	on atpD					885:891	Multigene phylogeny based on atpD and rpoB gene sequences	859:915	on atpD	885:891	Multigene phylogeny based on atpD and rpoB gene sequences confirmed it as a new species within the family Promicromonosporaceae (Phylum Actinobacteria).
33399896	15	22	theme	indicum	1677:1683	arg1	sp					1685:1686	Myceligenerans indicum sp	1662:1686	Myceligenerans indicum sp	1662:1686	The isolate I2 has been proposed as a new species, Myceligenerans indicum sp.
33399896	15	22	theme	indicum	1677:1683	arg1	species					1653:1659	a new species	1647:1659	a new species	1647:1659	The isolate I2 has been proposed as a new species, Myceligenerans indicum sp.
33399896	5	23	theme	pH	503:504	arg1	range					467:471	a wide range	460:471	a wide range of temperature, salinity, and pH	460:504	The organism can grow across a wide range of temperature, salinity, and pH as well as on different carbon sources.
33399896	0	24	theme	indicum	15:21	arg1	sp					23:24	indicum sp	15:24	indicum sp	15:24	Myceligenerans indicum sp.
33399896	14	25	theme	0.859	1531:1535	arg1	identity					1537:1544	0.859 identity	1531:1544	0.859 identity	1531:1544	GGDC (%), orthoANIu (%), and AAI of I2 genome indicated 28.9%, 77.44% and 0.859 identity with the genome of Myceligenerans xiligouense strain DSM 15,700.
33399896	8	26	theme	gene	902:905	arg1	sequences					907:915	Multigene phylogeny based on atpD and rpoB gene sequences	859:915	sequences	907:915	Multigene phylogeny based on atpD and rpoB gene sequences confirmed it as a new species within the family Promicromonosporaceae (Phylum Actinobacteria).
33399896	6	27	theme	identity	675:682	arg1	XHU					661:663	XHU	661:663	XHU	661:663	Phylogenetic analyses based on 16S rRNA showed that this isolate is closely related to Myceligenerans salitolerans XHU 5031 (99% identity; 100% coverage).
33399896	6	27	theme	identity	675:682	arg1	%					688:688	99% identity; 100% coverage	671:697	99% identity; 100% coverage	671:697	Phylogenetic analyses based on 16S rRNA showed that this isolate is closely related to Myceligenerans salitolerans XHU 5031 (99% identity; 100% coverage).
33399896	12	28	theme	fatty	1307:1311	arg1	acids					1313:1317	Major fatty acids	1301:1317	Major fatty acids present in the isolate	1301:1340	Major fatty acids present in the isolate are anteiso-C15, iso-C15, iso-C16, and anteiso-C17.
33399896	12	28	theme	fatty	1307:1311	arg1	anteiso-C15					1346:1356	anteiso-C15	1346:1356	anteiso-C15	1346:1356	Major fatty acids present in the isolate are anteiso-C15, iso-C15, iso-C16, and anteiso-C17.
33399896	14	29	theme	DSM	1599:1601	arg1	genome					1555:1560	the genome	1551:1560	the genome of Myceligenerans xiligouense strain DSM 15,700	1551:1608	GGDC (%), orthoANIu (%), and AAI of I2 genome indicated 28.9%, 77.44% and 0.859 identity with the genome of Myceligenerans xiligouense strain DSM 15,700.
33399896	4	30	theme	cocci-	399:404	arg1	elements					421:428	cocci- and rod-shaped elements	399:428	cocci- and rod-shaped elements	399:428	The isolate exhibit well-developed substrate mycelia along with the presence of cocci- and rod-shaped elements.
33399896	11	31	theme	polar	1191:1195	arg1	lipids					1197:1202	The polar lipids	1187:1202	The polar lipids	1187:1202	The polar lipids were represented by diphosphatidylglycerol, one unknown phospholipid and one unknown glycolipid.
33399896	6	32	theme	%	673:673	arg1	XHU					661:663	XHU	661:663	XHU	661:663	Phylogenetic analyses based on 16S rRNA showed that this isolate is closely related to Myceligenerans salitolerans XHU 5031 (99% identity; 100% coverage).
33399896	6	32	theme	%	673:673	arg1	%					688:688	99% identity; 100% coverage	671:697	99% identity; 100% coverage	671:697	Phylogenetic analyses based on 16S rRNA showed that this isolate is closely related to Myceligenerans salitolerans XHU 5031 (99% identity; 100% coverage).
33399896	12	33	theme	Major	1301:1305	arg1	acids					1313:1317	Major fatty acids	1301:1317	Major fatty acids present in the isolate	1301:1340	Major fatty acids present in the isolate are anteiso-C15, iso-C15, iso-C16, and anteiso-C17.
33399896	12	33	theme	Major	1301:1305	arg1	anteiso-C15					1346:1356	anteiso-C15	1346:1356	anteiso-C15	1346:1356	Major fatty acids present in the isolate are anteiso-C15, iso-C15, iso-C16, and anteiso-C17.
33399896	1	34	attach	isolated	52:59	arg2	actinobacterium					36:50	an actinobacterium	33:50	an actinobacterium isolated from mangrove sediment of Sundarbans, India	33:103	nov., an actinobacterium isolated from mangrove sediment of Sundarbans, India.
33399896	1	34	attach	isolated	52:59	arg1	sediment					75:82	mangrove sediment	66:82	mangrove sediment of Sundarbans, India	66:103	nov., an actinobacterium isolated from mangrove sediment of Sundarbans, India.
33399896	7	35	theme	ability	817:823	arg1	evidence					791:798	evidence	791:798	evidence of its potential ability to produce secondary metabolites	791:856	Presence of ketosynthase domain representing polyketide synthases in the isolate provides evidence of its potential ability to produce secondary metabolites.
33399896	5	36	theme	temperature	476:486	arg1	range					467:471	a wide range	460:471	a wide range of temperature, salinity, and pH	460:504	The organism can grow across a wide range of temperature, salinity, and pH as well as on different carbon sources.
33399896	17	37	theme	genome	1698:1703	arg1	sequence					1705:1712	The genome sequence	1694:1712	The genome sequence	1694:1712	The genome sequence has been deposited to GenBank/ENA/DDBJ under the accession number JABBYC000000000.
33399896	1	38	theme	India	99:103	arg1	sediment					75:82	mangrove sediment	66:82	mangrove sediment of Sundarbans, India	66:103	nov., an actinobacterium isolated from mangrove sediment of Sundarbans, India.
33399896	15	39	theme	isolate	1615:1621	arg1	I2					1623:1624	The isolate I2	1611:1624	The isolate I2	1611:1624	The isolate I2 has been proposed as a new species, Myceligenerans indicum sp.
33399896	15	39	theme	isolate	1615:1621	arg1	species					1653:1659	a new species	1647:1659	a new species	1647:1659	The isolate I2 has been proposed as a new species, Myceligenerans indicum sp.
33399896	1	40	dep	actinobacterium	36:50	arg1	nov.					27:30	nov.	27:30	nov.	27:30	nov., an actinobacterium isolated from mangrove sediment of Sundarbans, India.
33399896	6	41	theme	16S	577:579	arg1	rRNA					581:584	16S rRNA	577:584	16S rRNA	577:584	Phylogenetic analyses based on 16S rRNA showed that this isolate is closely related to Myceligenerans salitolerans XHU 5031 (99% identity; 100% coverage).
33399896	13	42	theme	Whole-genome	1394:1405	arg1	sequence					1407:1414	Whole-genome sequence	1394:1414	Whole-genome sequence	1394:1414	Whole-genome sequence indicates the size of genome is ~ 5 Mbp.
33399896	2	43	theme	mangrove	232:239	arg1	ecosystem					241:249	Indian Sundarbans mangrove ecosystem	214:249	Indian Sundarbans mangrove ecosystem	214:249	A new actinobacterial species of the genus Myceligenerans has been isolated from the intertidal sediment of Indian Sundarbans mangrove ecosystem.
33399896	5	44	theme	salinity	489:496	arg1	range					467:471	a wide range	460:471	a wide range of temperature, salinity, and pH	460:504	The organism can grow across a wide range of temperature, salinity, and pH as well as on different carbon sources.
33399896	12	45	attach	present	1319:1325	arg2	acids					1313:1317	Major fatty acids	1301:1317	Major fatty acids present in the isolate	1301:1340	Major fatty acids present in the isolate are anteiso-C15, iso-C15, iso-C16, and anteiso-C17.
33399896	12	45	attach	present	1319:1325	arg2	anteiso-C15					1346:1356	anteiso-C15	1346:1356	anteiso-C15	1346:1356	Major fatty acids present in the isolate are anteiso-C15, iso-C15, iso-C16, and anteiso-C17.
33399896	12	45	attach	present	1319:1325	arg1	isolate					1334:1340	the isolate	1330:1340	the isolate	1330:1340	Major fatty acids present in the isolate are anteiso-C15, iso-C15, iso-C16, and anteiso-C17.
33399896	7	46	from	Presence	701:708	arg1	isolate					774:780	the isolate	770:780	the isolate	770:780	Presence of ketosynthase domain representing polyketide synthases in the isolate provides evidence of its potential ability to produce secondary metabolites.
33399896	2	47	theme	Sundarbans	221:230	arg1	ecosystem					241:249	Indian Sundarbans mangrove ecosystem	214:249	Indian Sundarbans mangrove ecosystem	214:249	A new actinobacterial species of the genus Myceligenerans has been isolated from the intertidal sediment of Indian Sundarbans mangrove ecosystem.
33399896	2	48	attach	isolated	173:180	arg2	species					128:134	A new actinobacterial species	106:134	A new actinobacterial species of the genus Myceligenerans	106:162	A new actinobacterial species of the genus Myceligenerans has been isolated from the intertidal sediment of Indian Sundarbans mangrove ecosystem.
33399896	2	48	attach	isolated	173:180	arg1	sediment					202:209	the intertidal sediment	187:209	the intertidal sediment of Indian Sundarbans mangrove ecosystem	187:249	A new actinobacterial species of the genus Myceligenerans has been isolated from the intertidal sediment of Indian Sundarbans mangrove ecosystem.
33399896	10	49	theme	whole-cell	1120:1129	arg1	hydrolysates					1131:1142	the whole-cell hydrolysates	1116:1142	the whole-cell hydrolysates	1116:1142	The peptidoglycan type was A4α and the whole-cell hydrolysates contained glucose, galactose, and mannose.
33399896	2	50	theme	Myceligenerans	149:162	arg1	species					128:134	A new actinobacterial species	106:134	A new actinobacterial species of the genus Myceligenerans	106:162	A new actinobacterial species of the genus Myceligenerans has been isolated from the intertidal sediment of Indian Sundarbans mangrove ecosystem.
33399896	2	51	theme	Indian	214:219	arg1	ecosystem					241:249	Indian Sundarbans mangrove ecosystem	214:249	Indian Sundarbans mangrove ecosystem	214:249	A new actinobacterial species of the genus Myceligenerans has been isolated from the intertidal sediment of Indian Sundarbans mangrove ecosystem.
33399896	4	52	theme	substrate	354:362	arg1	mycelia					364:370	well-developed substrate mycelia	339:370	well-developed substrate mycelia	339:370	The isolate exhibit well-developed substrate mycelia along with the presence of cocci- and rod-shaped elements.
33399896	7	53	theme	polyketide	746:755	arg1	synthases					757:765	polyketide synthases	746:765	polyketide synthases	746:765	Presence of ketosynthase domain representing polyketide synthases in the isolate provides evidence of its potential ability to produce secondary metabolites.
33399896	11	54	theme	unknown	1252:1258	arg1	phospholipid					1260:1271	one unknown phospholipid	1248:1271	one unknown phospholipid	1248:1271	The polar lipids were represented by diphosphatidylglycerol, one unknown phospholipid and one unknown glycolipid.
33399896	11	54	theme	unknown	1252:1258	arg1	diphosphatidylglycerol					1224:1245	diphosphatidylglycerol	1224:1245	diphosphatidylglycerol	1224:1245	The polar lipids were represented by diphosphatidylglycerol, one unknown phospholipid and one unknown glycolipid.
33399896	8	55	theme	rpoB	897:900	arg1	sequences					907:915	Multigene phylogeny based on atpD and rpoB gene sequences	859:915	sequences	907:915	Multigene phylogeny based on atpD and rpoB gene sequences confirmed it as a new species within the family Promicromonosporaceae (Phylum Actinobacteria).
33399896	13	56	theme	genome	1438:1443	arg1	size					1430:1433	the size	1426:1433	the size of genome is ~ 5 Mbp	1426:1454	Whole-genome sequence indicates the size of genome is ~ 5 Mbp.
33399896	9	57	theme	DNA	1016:1018	arg1	content					1026:1032	The DNA G + C content	1012:1032	The DNA G + C content of the isolate	1012:1047	The DNA G + C content of the isolate has been determined as 72 mol%.
33399896	9	57	theme	DNA	1016:1018	arg1	%					1078:1078	72 mol%	1072:1078	72 mol%	1072:1078	The DNA G + C content of the isolate has been determined as 72 mol%.
33399896	8	58	theme	based	879:883	arg1	on atpD					885:891	Multigene phylogeny based on atpD and rpoB gene sequences	859:915	on atpD	885:891	Multigene phylogeny based on atpD and rpoB gene sequences confirmed it as a new species within the family Promicromonosporaceae (Phylum Actinobacteria).
33399896	17	59	theme	accession	1763:1771	arg1	number					1773:1778	the accession number JABBYC000000000	1759:1794	the accession number JABBYC000000000	1759:1794	The genome sequence has been deposited to GenBank/ENA/DDBJ under the accession number JABBYC000000000.
33399896	2	60	theme	intertidal	191:200	arg1	sediment					202:209	the intertidal sediment	187:209	the intertidal sediment of Indian Sundarbans mangrove ecosystem	187:249	A new actinobacterial species of the genus Myceligenerans has been isolated from the intertidal sediment of Indian Sundarbans mangrove ecosystem.
33399896	12	61	theme	present	1319:1325	arg1	acids					1313:1317	Major fatty acids	1301:1317	Major fatty acids present in the isolate	1301:1340	Major fatty acids present in the isolate are anteiso-C15, iso-C15, iso-C16, and anteiso-C17.
33399896	12	61	theme	present	1319:1325	arg1	anteiso-C15					1346:1356	anteiso-C15	1346:1356	anteiso-C15	1346:1356	Major fatty acids present in the isolate are anteiso-C15, iso-C15, iso-C16, and anteiso-C17.
33399896	14	62	theme	I2	1493:1494	arg1	genome					1496:1501	I2 genome	1493:1501	I2 genome	1493:1501	GGDC (%), orthoANIu (%), and AAI of I2 genome indicated 28.9%, 77.44% and 0.859 identity with the genome of Myceligenerans xiligouense strain DSM 15,700.
33399896	10	63	theme	peptidoglycan	1085:1097	arg1	A4α					1108:1110	A4α	1108:1110	A4α	1108:1110	The peptidoglycan type was A4α and the whole-cell hydrolysates contained glucose, galactose, and mannose.
33399896	10	63	theme	peptidoglycan	1085:1097	arg1	type					1099:1102	The peptidoglycan type	1081:1102	The peptidoglycan type	1081:1102	The peptidoglycan type was A4α and the whole-cell hydrolysates contained glucose, galactose, and mannose.
33399896	9	64	theme	G + C	1020:1024	arg1	content					1026:1032	The DNA G + C content	1012:1032	The DNA G + C content of the isolate	1012:1047	The DNA G + C content of the isolate has been determined as 72 mol%.
33399896	9	64	theme	G + C	1020:1024	arg1	%					1078:1078	72 mol%	1072:1078	72 mol%	1072:1078	The DNA G + C content of the isolate has been determined as 72 mol%.
33399896	7	65	theme	domain	726:731	arg1	Presence					701:708	Presence	701:708	Presence of ketosynthase domain representing polyketide synthases in the isolate	701:780	Presence of ketosynthase domain representing polyketide synthases in the isolate provides evidence of its potential ability to produce secondary metabolites.
33399896	3	66	theme	polyphasic	296:305	arg1	approaches					307:316	polyphasic approaches	296:316	polyphasic approaches	296:316	The isolate has been characterized based on polyphasic approaches.
33399896	0	67	dep	sp	23:24	arg1	Myceligenerans					0:13	Myceligenerans	0:13	Myceligenerans	0:13	Myceligenerans indicum sp.
33399896	13	68	theme	Mbp	1452:1454	arg1	size					1430:1433	the size	1426:1433	the size of genome is ~ 5 Mbp	1426:1454	Whole-genome sequence indicates the size of genome is ~ 5 Mbp.
33399896	5	69	theme	wide	462:465	arg1	range					467:471	a wide range	460:471	a wide range of temperature, salinity, and pH	460:504	The organism can grow across a wide range of temperature, salinity, and pH as well as on different carbon sources.
33399896	9	70	theme	72 mol	1072:1077	arg1	content					1026:1032	The DNA G + C content	1012:1032	The DNA G + C content of the isolate	1012:1047	The DNA G + C content of the isolate has been determined as 72 mol%.
33399896	9	70	theme	72 mol	1072:1077	arg1	%					1078:1078	72 mol%	1072:1078	72 mol%	1072:1078	The DNA G + C content of the isolate has been determined as 72 mol%.
33399896	6	71	theme	Phylogenetic	546:557	arg1	analyses					559:566	Phylogenetic analyses	546:566	Phylogenetic analyses based on 16S rRNA	546:584	Phylogenetic analyses based on 16S rRNA showed that this isolate is closely related to Myceligenerans salitolerans XHU 5031 (99% identity; 100% coverage).
33399896	14	72	theme	strain	1592:1597	arg1	DSM					1599:1601	Myceligenerans xiligouense strain DSM 15,700	1565:1608	Myceligenerans xiligouense strain DSM 15,700	1565:1608	GGDC (%), orthoANIu (%), and AAI of I2 genome indicated 28.9%, 77.44% and 0.859 identity with the genome of Myceligenerans xiligouense strain DSM 15,700.
33399896	8	73	theme	new	935:937	arg1	species					939:945	a new species	933:945	a new species within the family Promicromonosporaceae (Phylum Actinobacteria)	933:1009	Multigene phylogeny based on atpD and rpoB gene sequences confirmed it as a new species within the family Promicromonosporaceae (Phylum Actinobacteria).
33399896	8	73	theme	new	935:937	arg1	it					927:928	it	927:928	it	927:928	Multigene phylogeny based on atpD and rpoB gene sequences confirmed it as a new species within the family Promicromonosporaceae (Phylum Actinobacteria).
33399896	12	74	from	isolate	1334:1340	arg1	present					1319:1325	present	1319:1325	present	1319:1325	Major fatty acids present in the isolate are anteiso-C15, iso-C15, iso-C16, and anteiso-C17.
33399896	2	75	theme	actinobacterial	112:126	arg1	species					128:134	A new actinobacterial species	106:134	A new actinobacterial species of the genus Myceligenerans	106:162	A new actinobacterial species of the genus Myceligenerans has been isolated from the intertidal sediment of Indian Sundarbans mangrove ecosystem.
33399896	2	76	theme	ecosystem	241:249	arg1	sediment					202:209	the intertidal sediment	187:209	the intertidal sediment of Indian Sundarbans mangrove ecosystem	187:249	A new actinobacterial species of the genus Myceligenerans has been isolated from the intertidal sediment of Indian Sundarbans mangrove ecosystem.
33399896	4	77	theme	well-developed	339:352	arg1	mycelia					364:370	well-developed substrate mycelia	339:370	well-developed substrate mycelia	339:370	The isolate exhibit well-developed substrate mycelia along with the presence of cocci- and rod-shaped elements.
33399896	14	78	theme	genome	1496:1501	arg1	%					1478:1478	%	1478:1478	%	1478:1478	GGDC (%), orthoANIu (%), and AAI of I2 genome indicated 28.9%, 77.44% and 0.859 identity with the genome of Myceligenerans xiligouense strain DSM 15,700.
33399896	14	78	theme	genome	1496:1501	arg1	%					1463:1463	%	1463:1463	%	1463:1463	GGDC (%), orthoANIu (%), and AAI of I2 genome indicated 28.9%, 77.44% and 0.859 identity with the genome of Myceligenerans xiligouense strain DSM 15,700.
33399896	14	78	theme	genome	1496:1501	arg1	orthoANIu					1467:1475	orthoANIu	1467:1475	orthoANIu (%)	1467:1479	GGDC (%), orthoANIu (%), and AAI of I2 genome indicated 28.9%, 77.44% and 0.859 identity with the genome of Myceligenerans xiligouense strain DSM 15,700.
33399896	14	78	theme	genome	1496:1501	arg1	GGDC					1457:1460	GGDC	1457:1460	GGDC (%)	1457:1464	GGDC (%), orthoANIu (%), and AAI of I2 genome indicated 28.9%, 77.44% and 0.859 identity with the genome of Myceligenerans xiligouense strain DSM 15,700.
33399896	14	78	theme	genome	1496:1501	arg1	AAI					1486:1488	AAI	1486:1488	AAI of I2 genome	1486:1501	GGDC (%), orthoANIu (%), and AAI of I2 genome indicated 28.9%, 77.44% and 0.859 identity with the genome of Myceligenerans xiligouense strain DSM 15,700.
33399896	7	79	theme	ketosynthase	713:724	arg1	domain					726:731	ketosynthase domain	713:731	ketosynthase domain representing polyketide synthases	713:765	Presence of ketosynthase domain representing polyketide synthases in the isolate provides evidence of its potential ability to produce secondary metabolites.
32829806	0	0	theme	food	104:107	arg1	packaging					109:117	bioactive food packaging	94:117	bioactive food packaging	94:117	Antibacterial tragacanth gum-based nanocomposite films carrying ascorbic acid antioxidant for bioactive food packaging.
32829806	5	1	theme	AA	710:711	arg1	NPs					721:723	AA and ZnO NPs	710:723	AA and ZnO NPs	710:723	The incorporation of AA and ZnO NPs into nanocomposite films improved antioxidant activity from 50 % to 66 % in 95 % ethanolic solution.
32829806	5	2	from	%	796:796	arg1	solution					816:823	95 % ethanolic solution	801:823	95 % ethanolic solution	801:823	The incorporation of AA and ZnO NPs into nanocomposite films improved antioxidant activity from 50 % to 66 % in 95 % ethanolic solution.
32829806	0	3	theme	bioactive	94:102	arg1	packaging					109:117	bioactive food packaging	94:117	bioactive food packaging	94:117	Antibacterial tragacanth gum-based nanocomposite films carrying ascorbic acid antioxidant for bioactive food packaging.
32829806	1	4	contain	has	135:137	arg2	share					149:153	a pivotal share	139:153	a pivotal share	139:153	Food packaging has a pivotal share to improve protection, safety and shelf-life time of foods and bioproducts.
32829806	1	4	contain	has	135:137	arg1	packaging					125:133	Food packaging	120:133	Food packaging	120:133	Food packaging has a pivotal share to improve protection, safety and shelf-life time of foods and bioproducts.
32829806	7	5	theme	TG	1025:1026	arg1	%					1020:1020	the wt%	1014:1020	the wt% of TG	1014:1026	Soil degradation rate of nanocomposite films increased from 80 % to 91.46 as the wt% of TG increased.
32829806	7	5	theme	TG	1025:1026	arg1	TG					1025:1026	TG	1025:1026	TG	1025:1026	Soil degradation rate of nanocomposite films increased from 80 % to 91.46 as the wt% of TG increased.
32829806	3	6	theme	homogenous	491:500	arg1	distribution					502:513	a homogenous distribution	489:513	a homogenous distribution of ZnO NPs with low aggregation in nanocomposite films	489:568	The SEM images showed a homogenous distribution of ZnO NPs with low aggregation in nanocomposite films.
32829806	6	7	theme	-positive	917:925	arg1	bacteria					927:934	Gram-negative and -positive bacteria	899:934	Gram-negative and -positive bacteria	899:934	Also, the nanocomposite films showed good antibacterial activity against Gram-negative and -positive bacteria.
32829806	5	8	theme	50	785:786	arg1	%					788:788	%	788:788	%	788:788	The incorporation of AA and ZnO NPs into nanocomposite films improved antioxidant activity from 50 % to 66 % in 95 % ethanolic solution.
32829806	0	9	contain	carrying	55:62	arg2	acid					73:76	ascorbic acid	64:76	ascorbic acid antioxidant for bioactive food packaging	64:117	Antibacterial tragacanth gum-based nanocomposite films carrying ascorbic acid antioxidant for bioactive food packaging.
32829806	0	9	contain	carrying	55:62	arg1	films					49:53	nanocomposite films	35:53	nanocomposite films carrying ascorbic acid antioxidant for bioactive food packaging	35:117	Antibacterial tragacanth gum-based nanocomposite films carrying ascorbic acid antioxidant for bioactive food packaging.
32829806	1	10	theme	shelf-life	189:198	arg1	time					200:203	shelf-life time	189:203	shelf-life time	189:203	Food packaging has a pivotal share to improve protection, safety and shelf-life time of foods and bioproducts.
32829806	5	11	theme	66	793:794	arg1	%					788:788	%	788:788	%	788:788	The incorporation of AA and ZnO NPs into nanocomposite films improved antioxidant activity from 50 % to 66 % in 95 % ethanolic solution.
32829806	0	12	theme	ascorbic	64:71	arg1	acid					73:76	ascorbic acid	64:76	ascorbic acid antioxidant for bioactive food packaging	64:117	Antibacterial tragacanth gum-based nanocomposite films carrying ascorbic acid antioxidant for bioactive food packaging.
32829806	8	13	theme	packaging	1131:1139	arg1	applications					1141:1152	food packaging applications	1126:1152	food packaging applications	1126:1152	Therefore, prepared nanocomposite films could be employed as a promising candidate for food packaging applications.
32829806	8	14	theme	promising	1102:1110	arg1	candidate					1112:1120	a promising candidate	1100:1120	a promising candidate for food packaging applications	1100:1152	Therefore, prepared nanocomposite films could be employed as a promising candidate for food packaging applications.
32829806	8	14	theme	promising	1102:1110	arg1	films					1073:1077	prepared nanocomposite films	1050:1077	prepared nanocomposite films	1050:1077	Therefore, prepared nanocomposite films could be employed as a promising candidate for food packaging applications.
32829806	8	15	theme	nanocomposite	1059:1071	arg1	candidate					1112:1120	a promising candidate	1100:1120	a promising candidate for food packaging applications	1100:1152	Therefore, prepared nanocomposite films could be employed as a promising candidate for food packaging applications.
32829806	8	15	theme	nanocomposite	1059:1071	arg1	films					1073:1077	prepared nanocomposite films	1050:1077	prepared nanocomposite films	1050:1077	Therefore, prepared nanocomposite films could be employed as a promising candidate for food packaging applications.
32829806	3	16	theme	NPs	522:524	arg1	distribution					502:513	a homogenous distribution	489:513	a homogenous distribution of ZnO NPs with low aggregation in nanocomposite films	489:568	The SEM images showed a homogenous distribution of ZnO NPs with low aggregation in nanocomposite films.
32829806	2	17	theme	ascorbic	362:369	arg1	acid					371:374	ascorbic acid	362:374	ascorbic acid (AA)	362:379	Herein, we prepared bioactive nanocomposite films that composed of tragacanth (TG), polyvinyl alcohol, ZnO nanoparticles (NPs) and ascorbic acid (AA) using glycerol as a plasticizer and citric acid as a cross-linker for food packaging.
32829806	2	17	theme	ascorbic	362:369	arg1	AA					377:378	AA	377:378	AA	377:378	Herein, we prepared bioactive nanocomposite films that composed of tragacanth (TG), polyvinyl alcohol, ZnO nanoparticles (NPs) and ascorbic acid (AA) using glycerol as a plasticizer and citric acid as a cross-linker for food packaging.
32829806	0	18	theme	Antibacterial	0:12	arg1	tragacanth					14:23	Antibacterial tragacanth	0:23	Antibacterial tragacanth	0:23	Antibacterial tragacanth gum-based nanocomposite films carrying ascorbic acid antioxidant for bioactive food packaging.
32829806	7	19	theme	wt	1018:1019	arg1	%					1020:1020	the wt%	1014:1020	the wt% of TG	1014:1026	Soil degradation rate of nanocomposite films increased from 80 % to 91.46 as the wt% of TG increased.
32829806	7	19	theme	wt	1018:1019	arg1	TG					1025:1026	TG	1025:1026	TG	1025:1026	Soil degradation rate of nanocomposite films increased from 80 % to 91.46 as the wt% of TG increased.
32829806	4	20	theme	NPs	675:677	arg1	contents					679:686	ZnO NPs contents	671:686	ZnO NPs contents	671:686	The water solubility of nanocomposite films reduced from 15.65 % to 10.81 with increasing of TG and ZnO NPs contents.
32829806	5	21	theme	95	801:802	arg1	%					804:804	%	804:804	%	804:804	The incorporation of AA and ZnO NPs into nanocomposite films improved antioxidant activity from 50 % to 66 % in 95 % ethanolic solution.
32829806	0	22	theme	nanocomposite	35:47	arg1	films					49:53	nanocomposite films	35:53	nanocomposite films carrying ascorbic acid antioxidant for bioactive food packaging	35:117	Antibacterial tragacanth gum-based nanocomposite films carrying ascorbic acid antioxidant for bioactive food packaging.
32829806	6	23	theme	Gram-negative	899:911	arg1	bacteria					927:934	Gram-negative and -positive bacteria	899:934	Gram-negative and -positive bacteria	899:934	Also, the nanocomposite films showed good antibacterial activity against Gram-negative and -positive bacteria.
32829806	1	24	theme	Food	120:123	arg1	packaging					125:133	Food packaging	120:133	Food packaging	120:133	Food packaging has a pivotal share to improve protection, safety and shelf-life time of foods and bioproducts.
32829806	3	25	from	aggregation	535:545	arg1	films					564:568	nanocomposite films	550:568	nanocomposite films	550:568	The SEM images showed a homogenous distribution of ZnO NPs with low aggregation in nanocomposite films.
32829806	3	26	theme	ZnO	518:520	arg1	NPs					522:524	ZnO NPs	518:524	ZnO NPs	518:524	The SEM images showed a homogenous distribution of ZnO NPs with low aggregation in nanocomposite films.
32829806	1	27	theme	foods	208:212	arg1	safety					178:183	safety	178:183	safety	178:183	Food packaging has a pivotal share to improve protection, safety and shelf-life time of foods and bioproducts.
32829806	1	27	theme	foods	208:212	arg1	time					200:203	shelf-life time	189:203	shelf-life time	189:203	Food packaging has a pivotal share to improve protection, safety and shelf-life time of foods and bioproducts.
32829806	1	27	theme	foods	208:212	arg1	protection					166:175	protection	166:175	protection	166:175	Food packaging has a pivotal share to improve protection, safety and shelf-life time of foods and bioproducts.
32829806	5	28	theme	ZnO	717:719	arg1	NPs					721:723	AA and ZnO NPs	710:723	AA and ZnO NPs	710:723	The incorporation of AA and ZnO NPs into nanocomposite films improved antioxidant activity from 50 % to 66 % in 95 % ethanolic solution.
32829806	4	29	theme	films	609:613	arg1	solubility					581:590	The water solubility	571:590	The water solubility of nanocomposite films	571:613	The water solubility of nanocomposite films reduced from 15.65 % to 10.81 with increasing of TG and ZnO NPs contents.
32829806	5	30	theme	NPs	721:723	arg1	incorporation					693:705	The incorporation	689:705	The incorporation of AA and ZnO NPs into nanocomposite films	689:748	The incorporation of AA and ZnO NPs into nanocomposite films improved antioxidant activity from 50 % to 66 % in 95 % ethanolic solution.
32829806	6	31	theme	antibacterial	868:880	arg1	activity					882:889	good antibacterial activity	863:889	good antibacterial activity against Gram-negative and -positive bacteria	863:934	Also, the nanocomposite films showed good antibacterial activity against Gram-negative and -positive bacteria.
32829806	8	32	theme	food	1126:1129	arg1	applications					1141:1152	food packaging applications	1126:1152	food packaging applications	1126:1152	Therefore, prepared nanocomposite films could be employed as a promising candidate for food packaging applications.
32829806	3	33	theme	nanocomposite	550:562	arg1	films					564:568	nanocomposite films	550:568	nanocomposite films	550:568	The SEM images showed a homogenous distribution of ZnO NPs with low aggregation in nanocomposite films.
32829806	1	34	theme	bioproducts	218:228	arg1	safety					178:183	safety	178:183	safety	178:183	Food packaging has a pivotal share to improve protection, safety and shelf-life time of foods and bioproducts.
32829806	1	34	theme	bioproducts	218:228	arg1	time					200:203	shelf-life time	189:203	shelf-life time	189:203	Food packaging has a pivotal share to improve protection, safety and shelf-life time of foods and bioproducts.
32829806	1	34	theme	bioproducts	218:228	arg1	protection					166:175	protection	166:175	protection	166:175	Food packaging has a pivotal share to improve protection, safety and shelf-life time of foods and bioproducts.
32829806	5	35	theme	%	804:804	arg1	solution					816:823	95 % ethanolic solution	801:823	95 % ethanolic solution	801:823	The incorporation of AA and ZnO NPs into nanocomposite films improved antioxidant activity from 50 % to 66 % in 95 % ethanolic solution.
32829806	5	36	theme	nanocomposite	730:742	arg1	films					744:748	nanocomposite films	730:748	nanocomposite films	730:748	The incorporation of AA and ZnO NPs into nanocomposite films improved antioxidant activity from 50 % to 66 % in 95 % ethanolic solution.
32829806	0	37	theme	antioxidant	78:88	arg1	acid					73:76	ascorbic acid	64:76	ascorbic acid antioxidant for bioactive food packaging	64:117	Antibacterial tragacanth gum-based nanocomposite films carrying ascorbic acid antioxidant for bioactive food packaging.
32829806	3	38	theme	low	531:533	arg1	aggregation					535:545	low aggregation	531:545	low aggregation in nanocomposite films	531:568	The SEM images showed a homogenous distribution of ZnO NPs with low aggregation in nanocomposite films.
32829806	7	39	theme	nanocomposite	962:974	arg1	films					976:980	nanocomposite films	962:980	nanocomposite films	962:980	Soil degradation rate of nanocomposite films increased from 80 % to 91.46 as the wt% of TG increased.
32829806	2	40	theme	ZnO	334:336	arg1	NPs					353:355	NPs	353:355	NPs	353:355	Herein, we prepared bioactive nanocomposite films that composed of tragacanth (TG), polyvinyl alcohol, ZnO nanoparticles (NPs) and ascorbic acid (AA) using glycerol as a plasticizer and citric acid as a cross-linker for food packaging.
32829806	2	40	theme	ZnO	334:336	arg1	nanoparticles					338:350	ZnO nanoparticles	334:350	ZnO nanoparticles (NPs)	334:356	Herein, we prepared bioactive nanocomposite films that composed of tragacanth (TG), polyvinyl alcohol, ZnO nanoparticles (NPs) and ascorbic acid (AA) using glycerol as a plasticizer and citric acid as a cross-linker for food packaging.
32829806	2	41	theme	citric	417:422	arg1	acid					424:427	citric acid	417:427	citric acid	417:427	Herein, we prepared bioactive nanocomposite films that composed of tragacanth (TG), polyvinyl alcohol, ZnO nanoparticles (NPs) and ascorbic acid (AA) using glycerol as a plasticizer and citric acid as a cross-linker for food packaging.
32829806	5	42	theme	antioxidant	759:769	arg1	activity					771:778	antioxidant activity	759:778	antioxidant activity	759:778	The incorporation of AA and ZnO NPs into nanocomposite films improved antioxidant activity from 50 % to 66 % in 95 % ethanolic solution.
32829806	7	43	theme	degradation	942:952	arg1	rate					954:957	Soil degradation rate	937:957	Soil degradation rate of nanocomposite films	937:980	Soil degradation rate of nanocomposite films increased from 80 % to 91.46 as the wt% of TG increased.
32829806	7	44	theme	films	976:980	arg1	rate					954:957	Soil degradation rate	937:957	Soil degradation rate of nanocomposite films	937:980	Soil degradation rate of nanocomposite films increased from 80 % to 91.46 as the wt% of TG increased.
32829806	5	45	dep	%	788:788	arg1	to					790:791	to	790:791	to	790:791	The incorporation of AA and ZnO NPs into nanocomposite films improved antioxidant activity from 50 % to 66 % in 95 % ethanolic solution.
32829806	3	46	with	distribution	502:513	arg1	aggregation					535:545	low aggregation	531:545	low aggregation in nanocomposite films	531:568	The SEM images showed a homogenous distribution of ZnO NPs with low aggregation in nanocomposite films.
32829806	1	47	theme	pivotal	141:147	arg1	share					149:153	a pivotal share	139:153	a pivotal share	139:153	Food packaging has a pivotal share to improve protection, safety and shelf-life time of foods and bioproducts.
32829806	2	48	theme	nanocomposite	261:273	arg1	films					275:279	bioactive nanocomposite films	251:279	bioactive nanocomposite films that composed of tragacanth (TG), polyvinyl alcohol, ZnO nanoparticles (NPs) and ascorbic acid (AA)	251:379	Herein, we prepared bioactive nanocomposite films that composed of tragacanth (TG), polyvinyl alcohol, ZnO nanoparticles (NPs) and ascorbic acid (AA) using glycerol as a plasticizer and citric acid as a cross-linker for food packaging.
32829806	2	49	theme	polyvinyl	315:323	arg1	alcohol					325:331	polyvinyl alcohol	315:331	polyvinyl alcohol	315:331	Herein, we prepared bioactive nanocomposite films that composed of tragacanth (TG), polyvinyl alcohol, ZnO nanoparticles (NPs) and ascorbic acid (AA) using glycerol as a plasticizer and citric acid as a cross-linker for food packaging.
32829806	4	50	theme	ZnO	671:673	arg1	contents					679:686	ZnO NPs contents	671:686	ZnO NPs contents	671:686	The water solubility of nanocomposite films reduced from 15.65 % to 10.81 with increasing of TG and ZnO NPs contents.
32829806	8	51	theme	prepared	1050:1057	arg1	candidate					1112:1120	a promising candidate	1100:1120	a promising candidate for food packaging applications	1100:1152	Therefore, prepared nanocomposite films could be employed as a promising candidate for food packaging applications.
32829806	8	51	theme	prepared	1050:1057	arg1	films					1073:1077	prepared nanocomposite films	1050:1077	prepared nanocomposite films	1050:1077	Therefore, prepared nanocomposite films could be employed as a promising candidate for food packaging applications.
32829806	4	52	theme	nanocomposite	595:607	arg1	films					609:613	nanocomposite films	595:613	nanocomposite films	595:613	The water solubility of nanocomposite films reduced from 15.65 % to 10.81 with increasing of TG and ZnO NPs contents.
32829806	2	53	theme	bioactive	251:259	arg1	films					275:279	bioactive nanocomposite films	251:279	bioactive nanocomposite films that composed of tragacanth (TG), polyvinyl alcohol, ZnO nanoparticles (NPs) and ascorbic acid (AA)	251:379	Herein, we prepared bioactive nanocomposite films that composed of tragacanth (TG), polyvinyl alcohol, ZnO nanoparticles (NPs) and ascorbic acid (AA) using glycerol as a plasticizer and citric acid as a cross-linker for food packaging.
32829806	7	54	theme	Soil	937:940	arg1	rate					954:957	Soil degradation rate	937:957	Soil degradation rate of nanocomposite films	937:980	Soil degradation rate of nanocomposite films increased from 80 % to 91.46 as the wt% of TG increased.
32829806	3	55	theme	SEM	471:473	arg1	images					475:480	The SEM images	467:480	The SEM images	467:480	The SEM images showed a homogenous distribution of ZnO NPs with low aggregation in nanocomposite films.
32829806	4	56	theme	water	575:579	arg1	solubility					581:590	The water solubility	571:590	The water solubility of nanocomposite films	571:613	The water solubility of nanocomposite films reduced from 15.65 % to 10.81 with increasing of TG and ZnO NPs contents.
32829806	2	57	theme	food	451:454	arg1	packaging					456:464	food packaging	451:464	food packaging	451:464	Herein, we prepared bioactive nanocomposite films that composed of tragacanth (TG), polyvinyl alcohol, ZnO nanoparticles (NPs) and ascorbic acid (AA) using glycerol as a plasticizer and citric acid as a cross-linker for food packaging.
32829806	6	58	theme	nanocomposite	836:848	arg1	films					850:854	the nanocomposite films	832:854	the nanocomposite films	832:854	Also, the nanocomposite films showed good antibacterial activity against Gram-negative and -positive bacteria.
32829806	5	59	theme	ethanolic	806:814	arg1	solution					816:823	95 % ethanolic solution	801:823	95 % ethanolic solution	801:823	The incorporation of AA and ZnO NPs into nanocomposite films improved antioxidant activity from 50 % to 66 % in 95 % ethanolic solution.
32829806	6	60	theme	good	863:866	arg1	activity					882:889	good antibacterial activity	863:889	good antibacterial activity against Gram-negative and -positive bacteria	863:934	Also, the nanocomposite films showed good antibacterial activity against Gram-negative and -positive bacteria.
33512160	4	0	theme	multiple	941:948	arg1	stabilizers					950:960	multiple stabilizers	941:960	multiple stabilizers	941:960	The physicochemical stability, microstructure, rheological properties, and in vitro gastrointestinal digestion of Pickering emulsions were controlled by the addition sequence and mass ratio of multiple stabilizers, which showed the enhanced stability and delayed lipid digestion of the particle-biopolymer-surfactant-stabilized Pickering emulsions.
33512160	3	1	theme	lipophilic	721:730	arg1	nutraceuticals					732:745	lipophilic nutraceuticals	721:745	lipophilic nutraceuticals	721:745	It is the first time that three different types of emulsifiers have been used to synergistically stabilize food Pickering emulsions for delivery of lipophilic nutraceuticals.
33512160	1	2	theme	particles	264:272	arg1	presence					237:244	the presence	233:244	the presence of zein colloidal particles (ZCPs), propylene glycol alginate (PGA), and rhamnolipid (Rha) for delivery of β-carotene	233:362	Novel Pickering emulsions were stabilized by complex interfaces in the presence of zein colloidal particles (ZCPs), propylene glycol alginate (PGA), and rhamnolipid (Rha) for delivery of β-carotene.
33512160	6	3	theme	Rha	1255:1257	arg1	coexistence					1225:1235	The coexistence	1221:1235	The coexistence of ZCPs, PGA, and Rha	1221:1257	The coexistence of ZCPs, PGA, and Rha could induce the competitive displacement, multilayer deposition, and interparticle network at the interface.
33512160	3	4	theme	nutraceuticals	732:745	arg1	delivery					709:716	delivery	709:716	delivery of lipophilic nutraceuticals	709:745	It is the first time that three different types of emulsifiers have been used to synergistically stabilize food Pickering emulsions for delivery of lipophilic nutraceuticals.
33512160	6	5	theme	interparticle	1329:1341	arg1	network					1343:1349	interparticle network	1329:1349	interparticle network	1329:1349	The coexistence of ZCPs, PGA, and Rha could induce the competitive displacement, multilayer deposition, and interparticle network at the interface.
33512160	9	6	theme	controlled	1751:1760	arg1	digestion					1768:1776	controlled lipid digestion	1751:1776	controlled lipid digestion	1751:1776	The novel Pickering emulsion could be incorporated in foods as well as pharmaceuticals for controlled lipid digestion or targeted nutrient delivery purposes.
33512160	3	7	theme	first	583:587	arg1	time					589:592	the first time that three different types of emulsifiers have been used to synergistically stabilize food Pickering emulsions for delivery of lipophilic nutraceuticals	579:745	the first time that three different types of emulsifiers have been used to synergistically stabilize food Pickering emulsions for delivery of lipophilic nutraceuticals	579:745	It is the first time that three different types of emulsifiers have been used to synergistically stabilize food Pickering emulsions for delivery of lipophilic nutraceuticals.
33512160	3	7	theme	first	583:587	arg1	It					573:574	It	573:574	It	573:574	It is the first time that three different types of emulsifiers have been used to synergistically stabilize food Pickering emulsions for delivery of lipophilic nutraceuticals.
33512160	4	8	dep	in	823:824	arg1	vitro					826:830	vitro	826:830	vitro	826:830	The physicochemical stability, microstructure, rheological properties, and in vitro gastrointestinal digestion of Pickering emulsions were controlled by the addition sequence and mass ratio of multiple stabilizers, which showed the enhanced stability and delayed lipid digestion of the particle-biopolymer-surfactant-stabilized Pickering emulsions.
33512160	9	9	theme	digestion	1768:1776	arg1	purposes					1808:1815	controlled lipid digestion or targeted nutrient delivery purposes	1751:1815	controlled lipid digestion or targeted nutrient delivery purposes	1751:1815	The novel Pickering emulsion could be incorporated in foods as well as pharmaceuticals for controlled lipid digestion or targeted nutrient delivery purposes.
33512160	1	10	theme	complex	211:217	arg1	interfaces					219:228	complex interfaces	211:228	complex interfaces	211:228	Novel Pickering emulsions were stabilized by complex interfaces in the presence of zein colloidal particles (ZCPs), propylene glycol alginate (PGA), and rhamnolipid (Rha) for delivery of β-carotene.
33512160	4	11	theme	enhanced	980:987	arg1	stability					989:997	enhanced stability	980:997	enhanced stability	980:997	The physicochemical stability, microstructure, rheological properties, and in vitro gastrointestinal digestion of Pickering emulsions were controlled by the addition sequence and mass ratio of multiple stabilizers, which showed the enhanced stability and delayed lipid digestion of the particle-biopolymer-surfactant-stabilized Pickering emulsions.
33512160	4	12	dep	stability	989:997	arg1	the					976:978	the	976:978	the	976:978	The physicochemical stability, microstructure, rheological properties, and in vitro gastrointestinal digestion of Pickering emulsions were controlled by the addition sequence and mass ratio of multiple stabilizers, which showed the enhanced stability and delayed lipid digestion of the particle-biopolymer-surfactant-stabilized Pickering emulsions.
33512160	3	13	theme	different	605:613	arg1	types					615:619	three different types	599:619	three different types of emulsifiers	599:634	It is the first time that three different types of emulsifiers have been used to synergistically stabilize food Pickering emulsions for delivery of lipophilic nutraceuticals.
33512160	9	14	theme	delivery	1799:1806	arg1	purposes					1808:1815	controlled lipid digestion or targeted nutrient delivery purposes	1751:1815	controlled lipid digestion or targeted nutrient delivery purposes	1751:1815	The novel Pickering emulsion could be incorporated in foods as well as pharmaceuticals for controlled lipid digestion or targeted nutrient delivery purposes.
33512160	6	15	theme	competitive	1276:1286	arg1	displacement					1288:1299	the competitive displacement	1272:1299	the competitive displacement	1272:1299	The coexistence of ZCPs, PGA, and Rha could induce the competitive displacement, multilayer deposition, and interparticle network at the interface.
33512160	7	16	theme	surfactant	1419:1428	arg1	combination					1373:1383	The combination	1369:1383	The combination of particles, a biopolymer, and a surfactant	1369:1428	The combination of particles, a biopolymer, and a surfactant delayed the lipolysis during in vitro gastrointestinal tract.
33512160	4	17	theme	physicochemical	752:766	arg1	stability					768:776	physicochemical stability	752:776	physicochemical stability	752:776	The physicochemical stability, microstructure, rheological properties, and in vitro gastrointestinal digestion of Pickering emulsions were controlled by the addition sequence and mass ratio of multiple stabilizers, which showed the enhanced stability and delayed lipid digestion of the particle-biopolymer-surfactant-stabilized Pickering emulsions.
33512160	5	18	theme	retention	1147:1155	arg1	rate					1157:1160	the retention rate	1143:1160	the retention rate of β-carotene	1143:1174	After encapsulation into Pickering emulsions, the retention rate of β-carotene increased 2-fold under UV radiation for 8 h.
33512160	4	19	dep	stability	768:776	arg1	The					748:750	The	748:750	The	748:750	The physicochemical stability, microstructure, rheological properties, and in vitro gastrointestinal digestion of Pickering emulsions were controlled by the addition sequence and mass ratio of multiple stabilizers, which showed the enhanced stability and delayed lipid digestion of the particle-biopolymer-surfactant-stabilized Pickering emulsions.
33512160	8	20	theme	free	1555:1558	arg1	acids					1566:1570	free fatty acids	1555:1570	free fatty acids from Pickering emulsions	1555:1595	By modulating the interfacial composition, the release rate of free fatty acids from Pickering emulsions was reduced from 19.46% to 2.83% through different mechanisms.
33512160	2	21	theme	physiochemical	487:500	arg1	properties					502:511	physiochemical properties	487:511	physiochemical properties	487:511	The influence of the particle-surfactant, particle-biopolymer, and particle-biopolymer-surfactant mixed interfaces on the physiochemical properties and digestion fate of Pickering emulsions was investigated.
33512160	8	22	theme	acids	1566:1570	arg1	rate					1547:1550	the release rate	1535:1550	the release rate of free fatty acids from Pickering emulsions	1535:1595	By modulating the interfacial composition, the release rate of free fatty acids from Pickering emulsions was reduced from 19.46% to 2.83% through different mechanisms.
33512160	0	23	theme	Pickering	86:94	arg1	Emulsions					96:104	β-Carotene-Loaded Pickering Emulsions	68:104	β-Carotene-Loaded Pickering Emulsions	68:104	Stability, Interfacial Structure, and Gastrointestinal Digestion of β-Carotene-Loaded Pickering Emulsions Co-stabilized by Particles, a Biopolymer, and a Surfactant.
33512160	1	24	theme	rhamnolipid	319:329	arg1	presence					237:244	the presence	233:244	the presence of zein colloidal particles (ZCPs), propylene glycol alginate (PGA), and rhamnolipid (Rha) for delivery of β-carotene	233:362	Novel Pickering emulsions were stabilized by complex interfaces in the presence of zein colloidal particles (ZCPs), propylene glycol alginate (PGA), and rhamnolipid (Rha) for delivery of β-carotene.
33512160	1	25	theme	Novel	166:170	arg1	emulsions					182:190	Novel Pickering emulsions	166:190	Novel Pickering emulsions	166:190	Novel Pickering emulsions were stabilized by complex interfaces in the presence of zein colloidal particles (ZCPs), propylene glycol alginate (PGA), and rhamnolipid (Rha) for delivery of β-carotene.
33512160	2	26	theme	Pickering	535:543	arg1	emulsions					545:553	Pickering emulsions	535:553	Pickering emulsions	535:553	The influence of the particle-surfactant, particle-biopolymer, and particle-biopolymer-surfactant mixed interfaces on the physiochemical properties and digestion fate of Pickering emulsions was investigated.
33512160	8	27	theme	Pickering	1577:1585	arg1	emulsions					1587:1595	Pickering emulsions	1577:1595	Pickering emulsions	1577:1595	By modulating the interfacial composition, the release rate of free fatty acids from Pickering emulsions was reduced from 19.46% to 2.83% through different mechanisms.
33512160	4	28	theme	lipid	1011:1015	arg1	digestion					1017:1025	delayed lipid digestion	1003:1025	delayed lipid digestion	1003:1025	The physicochemical stability, microstructure, rheological properties, and in vitro gastrointestinal digestion of Pickering emulsions were controlled by the addition sequence and mass ratio of multiple stabilizers, which showed the enhanced stability and delayed lipid digestion of the particle-biopolymer-surfactant-stabilized Pickering emulsions.
33512160	8	29	from	rate	1547:1550	arg1	emulsions					1587:1595	Pickering emulsions	1577:1595	Pickering emulsions	1577:1595	By modulating the interfacial composition, the release rate of free fatty acids from Pickering emulsions was reduced from 19.46% to 2.83% through different mechanisms.
33512160	2	30	theme	particle-biopolymer	407:425	arg1	interfaces					469:478	the particle-surfactant, particle-biopolymer, and particle-biopolymer-surfactant mixed interfaces	382:478	the particle-surfactant, particle-biopolymer, and particle-biopolymer-surfactant mixed interfaces	382:478	The influence of the particle-surfactant, particle-biopolymer, and particle-biopolymer-surfactant mixed interfaces on the physiochemical properties and digestion fate of Pickering emulsions was investigated.
33512160	2	31	theme	particle-surfactant	386:404	arg1	interfaces					469:478	the particle-surfactant, particle-biopolymer, and particle-biopolymer-surfactant mixed interfaces	382:478	the particle-surfactant, particle-biopolymer, and particle-biopolymer-surfactant mixed interfaces	382:478	The influence of the particle-surfactant, particle-biopolymer, and particle-biopolymer-surfactant mixed interfaces on the physiochemical properties and digestion fate of Pickering emulsions was investigated.
33512160	0	32	theme	Interfacial	11:21	arg1	Structure					23:31	Interfacial Structure	11:31	Interfacial Structure	11:31	Stability, Interfacial Structure, and Gastrointestinal Digestion of β-Carotene-Loaded Pickering Emulsions Co-stabilized by Particles, a Biopolymer, and a Surfactant.
33512160	1	33	theme	propylene	282:290	arg1	glycol					292:297	propylene glycol	282:297	propylene glycol alginate (PGA)	282:312	Novel Pickering emulsions were stabilized by complex interfaces in the presence of zein colloidal particles (ZCPs), propylene glycol alginate (PGA), and rhamnolipid (Rha) for delivery of β-carotene.
33512160	7	34	theme	particles	1388:1396	arg1	combination					1373:1383	The combination	1369:1383	The combination of particles, a biopolymer, and a surfactant	1369:1428	The combination of particles, a biopolymer, and a surfactant delayed the lipolysis during in vitro gastrointestinal tract.
33512160	4	35	theme	emulsions	1086:1094	arg1	stability					989:997	enhanced stability	980:997	enhanced stability	980:997	The physicochemical stability, microstructure, rheological properties, and in vitro gastrointestinal digestion of Pickering emulsions were controlled by the addition sequence and mass ratio of multiple stabilizers, which showed the enhanced stability and delayed lipid digestion of the particle-biopolymer-surfactant-stabilized Pickering emulsions.
33512160	4	35	theme	emulsions	1086:1094	arg1	digestion					1017:1025	delayed lipid digestion	1003:1025	delayed lipid digestion	1003:1025	The physicochemical stability, microstructure, rheological properties, and in vitro gastrointestinal digestion of Pickering emulsions were controlled by the addition sequence and mass ratio of multiple stabilizers, which showed the enhanced stability and delayed lipid digestion of the particle-biopolymer-surfactant-stabilized Pickering emulsions.
33512160	8	36	theme	2.83	1624:1627	arg1	%					1619:1619	%	1619:1619	%	1619:1619	By modulating the interfacial composition, the release rate of free fatty acids from Pickering emulsions was reduced from 19.46% to 2.83% through different mechanisms.
33512160	4	37	theme	Pickering	862:870	arg1	emulsions					872:880	Pickering emulsions	862:880	Pickering emulsions	862:880	The physicochemical stability, microstructure, rheological properties, and in vitro gastrointestinal digestion of Pickering emulsions were controlled by the addition sequence and mass ratio of multiple stabilizers, which showed the enhanced stability and delayed lipid digestion of the particle-biopolymer-surfactant-stabilized Pickering emulsions.
33512160	1	38	theme	alginate	299:306	arg1	presence					237:244	the presence	233:244	the presence of zein colloidal particles (ZCPs), propylene glycol alginate (PGA), and rhamnolipid (Rha) for delivery of β-carotene	233:362	Novel Pickering emulsions were stabilized by complex interfaces in the presence of zein colloidal particles (ZCPs), propylene glycol alginate (PGA), and rhamnolipid (Rha) for delivery of β-carotene.
33512160	2	39	theme	interfaces	469:478	arg1	influence					369:377	The influence	365:377	The influence of the particle-surfactant, particle-biopolymer, and particle-biopolymer-surfactant mixed interfaces on the physiochemical properties and digestion fate of Pickering emulsions	365:553	The influence of the particle-surfactant, particle-biopolymer, and particle-biopolymer-surfactant mixed interfaces on the physiochemical properties and digestion fate of Pickering emulsions was investigated.
33512160	9	40	theme	novel	1664:1668	arg1	emulsion					1680:1687	The novel Pickering emulsion	1660:1687	The novel Pickering emulsion	1660:1687	The novel Pickering emulsion could be incorporated in foods as well as pharmaceuticals for controlled lipid digestion or targeted nutrient delivery purposes.
33512160	2	41	theme	mixed	463:467	arg1	interfaces					469:478	the particle-surfactant, particle-biopolymer, and particle-biopolymer-surfactant mixed interfaces	382:478	the particle-surfactant, particle-biopolymer, and particle-biopolymer-surfactant mixed interfaces	382:478	The influence of the particle-surfactant, particle-biopolymer, and particle-biopolymer-surfactant mixed interfaces on the physiochemical properties and digestion fate of Pickering emulsions was investigated.
33512160	0	42	theme	Gastrointestinal	38:53	arg1	Digestion					55:63	Gastrointestinal Digestion	38:63	Gastrointestinal Digestion	38:63	Stability, Interfacial Structure, and Gastrointestinal Digestion of β-Carotene-Loaded Pickering Emulsions Co-stabilized by Particles, a Biopolymer, and a Surfactant.
33512160	2	43	dep	properties	502:511	arg1	the					483:485	the	483:485	the	483:485	The influence of the particle-surfactant, particle-biopolymer, and particle-biopolymer-surfactant mixed interfaces on the physiochemical properties and digestion fate of Pickering emulsions was investigated.
33512160	3	44	theme	food	680:683	arg1	emulsions					695:703	food Pickering emulsions	680:703	food Pickering emulsions for delivery of lipophilic nutraceuticals	680:745	It is the first time that three different types of emulsifiers have been used to synergistically stabilize food Pickering emulsions for delivery of lipophilic nutraceuticals.
33512160	8	45	theme	interfacial	1510:1520	arg1	composition					1522:1532	the interfacial composition	1506:1532	the interfacial composition	1506:1532	By modulating the interfacial composition, the release rate of free fatty acids from Pickering emulsions was reduced from 19.46% to 2.83% through different mechanisms.
33512160	4	46	theme	particle-biopolymer-surfactant-stabilized	1034:1074	arg1	emulsions					1086:1094	the particle-biopolymer-surfactant-stabilized Pickering emulsions	1030:1094	the particle-biopolymer-surfactant-stabilized Pickering emulsions	1030:1094	The physicochemical stability, microstructure, rheological properties, and in vitro gastrointestinal digestion of Pickering emulsions were controlled by the addition sequence and mass ratio of multiple stabilizers, which showed the enhanced stability and delayed lipid digestion of the particle-biopolymer-surfactant-stabilized Pickering emulsions.
33512160	1	47	theme	colloidal	254:262	arg1	particles					264:272	zein colloidal particles	249:272	zein colloidal particles (ZCPs)	249:279	Novel Pickering emulsions were stabilized by complex interfaces in the presence of zein colloidal particles (ZCPs), propylene glycol alginate (PGA), and rhamnolipid (Rha) for delivery of β-carotene.
33512160	1	47	theme	colloidal	254:262	arg1	ZCPs					275:278	ZCPs	275:278	ZCPs	275:278	Novel Pickering emulsions were stabilized by complex interfaces in the presence of zein colloidal particles (ZCPs), propylene glycol alginate (PGA), and rhamnolipid (Rha) for delivery of β-carotene.
33512160	9	48	theme	Pickering	1670:1678	arg1	emulsion					1680:1687	The novel Pickering emulsion	1660:1687	The novel Pickering emulsion	1660:1687	The novel Pickering emulsion could be incorporated in foods as well as pharmaceuticals for controlled lipid digestion or targeted nutrient delivery purposes.
33512160	4	49	theme	mass	927:930	arg1	ratio					932:936	mass ratio	927:936	mass ratio	927:936	The physicochemical stability, microstructure, rheological properties, and in vitro gastrointestinal digestion of Pickering emulsions were controlled by the addition sequence and mass ratio of multiple stabilizers, which showed the enhanced stability and delayed lipid digestion of the particle-biopolymer-surfactant-stabilized Pickering emulsions.
33512160	1	50	theme	Pickering	172:180	arg1	emulsions					182:190	Novel Pickering emulsions	166:190	Novel Pickering emulsions	166:190	Novel Pickering emulsions were stabilized by complex interfaces in the presence of zein colloidal particles (ZCPs), propylene glycol alginate (PGA), and rhamnolipid (Rha) for delivery of β-carotene.
33512160	2	51	theme	particle-biopolymer-surfactant	432:461	arg1	interfaces					469:478	the particle-surfactant, particle-biopolymer, and particle-biopolymer-surfactant mixed interfaces	382:478	the particle-surfactant, particle-biopolymer, and particle-biopolymer-surfactant mixed interfaces	382:478	The influence of the particle-surfactant, particle-biopolymer, and particle-biopolymer-surfactant mixed interfaces on the physiochemical properties and digestion fate of Pickering emulsions was investigated.
33512160	6	52	theme	PGA	1246:1248	arg1	coexistence					1225:1235	The coexistence	1221:1235	The coexistence of ZCPs, PGA, and Rha	1221:1257	The coexistence of ZCPs, PGA, and Rha could induce the competitive displacement, multilayer deposition, and interparticle network at the interface.
33512160	9	53	theme	lipid	1762:1766	arg1	digestion					1768:1776	controlled lipid digestion	1751:1776	controlled lipid digestion	1751:1776	The novel Pickering emulsion could be incorporated in foods as well as pharmaceuticals for controlled lipid digestion or targeted nutrient delivery purposes.
33512160	6	54	theme	ZCPs	1240:1243	arg1	coexistence					1225:1235	The coexistence	1221:1235	The coexistence of ZCPs, PGA, and Rha	1221:1257	The coexistence of ZCPs, PGA, and Rha could induce the competitive displacement, multilayer deposition, and interparticle network at the interface.
33512160	7	55	theme	gastrointestinal	1468:1483	arg1	tract					1485:1489	in vitro gastrointestinal tract	1459:1489	in vitro gastrointestinal tract	1459:1489	The combination of particles, a biopolymer, and a surfactant delayed the lipolysis during in vitro gastrointestinal tract.
33512160	1	56	theme	β-carotene	353:362	arg1	delivery					341:348	delivery	341:348	delivery of β-carotene	341:362	Novel Pickering emulsions were stabilized by complex interfaces in the presence of zein colloidal particles (ZCPs), propylene glycol alginate (PGA), and rhamnolipid (Rha) for delivery of β-carotene.
33512160	7	57	dep	in	1459:1460	arg1	vitro					1462:1466	vitro	1462:1466	vitro	1462:1466	The combination of particles, a biopolymer, and a surfactant delayed the lipolysis during in vitro gastrointestinal tract.
33512160	9	58	theme	nutrient	1790:1797	arg1	delivery					1799:1806	targeted nutrient delivery	1781:1806	targeted nutrient delivery	1781:1806	The novel Pickering emulsion could be incorporated in foods as well as pharmaceuticals for controlled lipid digestion or targeted nutrient delivery purposes.
33512160	8	59	theme	release	1539:1545	arg1	rate					1547:1550	the release rate	1535:1550	the release rate of free fatty acids from Pickering emulsions	1535:1595	By modulating the interfacial composition, the release rate of free fatty acids from Pickering emulsions was reduced from 19.46% to 2.83% through different mechanisms.
33512160	7	60	theme	in	1459:1460	arg1	tract					1485:1489	in vitro gastrointestinal tract	1459:1489	in vitro gastrointestinal tract	1459:1489	The combination of particles, a biopolymer, and a surfactant delayed the lipolysis during in vitro gastrointestinal tract.
33512160	3	61	theme	emulsifiers	624:634	arg1	types					615:619	three different types	599:619	three different types of emulsifiers	599:634	It is the first time that three different types of emulsifiers have been used to synergistically stabilize food Pickering emulsions for delivery of lipophilic nutraceuticals.
33512160	4	62	theme	stabilizers	950:960	arg1	addition					905:912	the addition sequence and mass ratio	901:936	addition	905:912	The physicochemical stability, microstructure, rheological properties, and in vitro gastrointestinal digestion of Pickering emulsions were controlled by the addition sequence and mass ratio of multiple stabilizers, which showed the enhanced stability and delayed lipid digestion of the particle-biopolymer-surfactant-stabilized Pickering emulsions.
33512160	4	62	theme	stabilizers	950:960	arg1	ratio					932:936	mass ratio	927:936	mass ratio	927:936	The physicochemical stability, microstructure, rheological properties, and in vitro gastrointestinal digestion of Pickering emulsions were controlled by the addition sequence and mass ratio of multiple stabilizers, which showed the enhanced stability and delayed lipid digestion of the particle-biopolymer-surfactant-stabilized Pickering emulsions.
33512160	8	63	theme	fatty	1560:1564	arg1	acids					1566:1570	free fatty acids	1555:1570	free fatty acids from Pickering emulsions	1555:1595	By modulating the interfacial composition, the release rate of free fatty acids from Pickering emulsions was reduced from 19.46% to 2.83% through different mechanisms.
33512160	0	64	theme	β-Carotene-Loaded	68:84	arg1	Emulsions					96:104	β-Carotene-Loaded Pickering Emulsions	68:104	β-Carotene-Loaded Pickering Emulsions	68:104	Stability, Interfacial Structure, and Gastrointestinal Digestion of β-Carotene-Loaded Pickering Emulsions Co-stabilized by Particles, a Biopolymer, and a Surfactant.
33512160	8	65	dep	%	1619:1619	arg1	to					1621:1622	to	1621:1622	to	1621:1622	By modulating the interfacial composition, the release rate of free fatty acids from Pickering emulsions was reduced from 19.46% to 2.83% through different mechanisms.
33512160	0	66	theme	Emulsions	96:104	arg1	Stability					0:8	Stability	0:8	Stability	0:8	Stability, Interfacial Structure, and Gastrointestinal Digestion of β-Carotene-Loaded Pickering Emulsions Co-stabilized by Particles, a Biopolymer, and a Surfactant.
33512160	0	66	theme	Emulsions	96:104	arg1	Structure					23:31	Interfacial Structure	11:31	Interfacial Structure	11:31	Stability, Interfacial Structure, and Gastrointestinal Digestion of β-Carotene-Loaded Pickering Emulsions Co-stabilized by Particles, a Biopolymer, and a Surfactant.
33512160	0	66	theme	Emulsions	96:104	arg1	Digestion					55:63	Gastrointestinal Digestion	38:63	Gastrointestinal Digestion	38:63	Stability, Interfacial Structure, and Gastrointestinal Digestion of β-Carotene-Loaded Pickering Emulsions Co-stabilized by Particles, a Biopolymer, and a Surfactant.
33512160	4	67	theme	rheological	795:805	arg1	properties					807:816	rheological properties	795:816	rheological properties	795:816	The physicochemical stability, microstructure, rheological properties, and in vitro gastrointestinal digestion of Pickering emulsions were controlled by the addition sequence and mass ratio of multiple stabilizers, which showed the enhanced stability and delayed lipid digestion of the particle-biopolymer-surfactant-stabilized Pickering emulsions.
33512160	5	68	theme	UV	1199:1200	arg1	radiation					1202:1210	UV radiation	1199:1210	UV radiation	1199:1210	After encapsulation into Pickering emulsions, the retention rate of β-carotene increased 2-fold under UV radiation for 8 h.
33512160	7	69	theme	biopolymer	1401:1410	arg1	combination					1373:1383	The combination	1369:1383	The combination of particles, a biopolymer, and a surfactant	1369:1428	The combination of particles, a biopolymer, and a surfactant delayed the lipolysis during in vitro gastrointestinal tract.
33512160	2	70	theme	digestion	517:525	arg1	fate					527:530	digestion fate	517:530	digestion fate	517:530	The influence of the particle-surfactant, particle-biopolymer, and particle-biopolymer-surfactant mixed interfaces on the physiochemical properties and digestion fate of Pickering emulsions was investigated.
33512160	8	71	theme	19.46	1614:1618	arg1	%					1619:1619	%	1619:1619	%	1619:1619	By modulating the interfacial composition, the release rate of free fatty acids from Pickering emulsions was reduced from 19.46% to 2.83% through different mechanisms.
33512160	4	72	dep	addition	905:912	arg1	sequence					914:921	sequence	914:921	sequence	914:921	The physicochemical stability, microstructure, rheological properties, and in vitro gastrointestinal digestion of Pickering emulsions were controlled by the addition sequence and mass ratio of multiple stabilizers, which showed the enhanced stability and delayed lipid digestion of the particle-biopolymer-surfactant-stabilized Pickering emulsions.
33512160	9	73	theme	targeted	1781:1788	arg1	delivery					1799:1806	targeted nutrient delivery	1781:1806	targeted nutrient delivery	1781:1806	The novel Pickering emulsion could be incorporated in foods as well as pharmaceuticals for controlled lipid digestion or targeted nutrient delivery purposes.
33512160	3	74	used	used	646:649	arg2	types					615:619	three different types	599:619	three different types of emulsifiers	599:634	It is the first time that three different types of emulsifiers have been used to synergistically stabilize food Pickering emulsions for delivery of lipophilic nutraceuticals.
33512160	5	75	theme	β-carotene	1165:1174	arg1	rate					1157:1160	the retention rate	1143:1160	the retention rate of β-carotene	1143:1174	After encapsulation into Pickering emulsions, the retention rate of β-carotene increased 2-fold under UV radiation for 8 h.
33512160	1	76	theme	glycol	292:297	arg1	PGA					309:311	PGA	309:311	PGA	309:311	Novel Pickering emulsions were stabilized by complex interfaces in the presence of zein colloidal particles (ZCPs), propylene glycol alginate (PGA), and rhamnolipid (Rha) for delivery of β-carotene.
33512160	1	76	theme	glycol	292:297	arg1	alginate					299:306	propylene glycol alginate	282:306	propylene glycol alginate (PGA)	282:312	Novel Pickering emulsions were stabilized by complex interfaces in the presence of zein colloidal particles (ZCPs), propylene glycol alginate (PGA), and rhamnolipid (Rha) for delivery of β-carotene.
33512160	4	77	theme	Pickering	1076:1084	arg1	emulsions					1086:1094	the particle-biopolymer-surfactant-stabilized Pickering emulsions	1030:1094	the particle-biopolymer-surfactant-stabilized Pickering emulsions	1030:1094	The physicochemical stability, microstructure, rheological properties, and in vitro gastrointestinal digestion of Pickering emulsions were controlled by the addition sequence and mass ratio of multiple stabilizers, which showed the enhanced stability and delayed lipid digestion of the particle-biopolymer-surfactant-stabilized Pickering emulsions.
33512160	8	78	theme	different	1638:1646	arg1	mechanisms					1648:1657	different mechanisms	1638:1657	different mechanisms	1638:1657	By modulating the interfacial composition, the release rate of free fatty acids from Pickering emulsions was reduced from 19.46% to 2.83% through different mechanisms.
33512160	4	79	theme	delayed	1003:1009	arg1	digestion					1017:1025	delayed lipid digestion	1003:1025	delayed lipid digestion	1003:1025	The physicochemical stability, microstructure, rheological properties, and in vitro gastrointestinal digestion of Pickering emulsions were controlled by the addition sequence and mass ratio of multiple stabilizers, which showed the enhanced stability and delayed lipid digestion of the particle-biopolymer-surfactant-stabilized Pickering emulsions.
33512160	5	80	theme	Pickering	1122:1130	arg1	emulsions					1132:1140	Pickering emulsions	1122:1140	Pickering emulsions	1122:1140	After encapsulation into Pickering emulsions, the retention rate of β-carotene increased 2-fold under UV radiation for 8 h.
33512160	2	81	theme	emulsions	545:553	arg1	properties					502:511	physiochemical properties	487:511	physiochemical properties	487:511	The influence of the particle-surfactant, particle-biopolymer, and particle-biopolymer-surfactant mixed interfaces on the physiochemical properties and digestion fate of Pickering emulsions was investigated.
33512160	2	81	theme	emulsions	545:553	arg1	fate					527:530	digestion fate	517:530	digestion fate	517:530	The influence of the particle-surfactant, particle-biopolymer, and particle-biopolymer-surfactant mixed interfaces on the physiochemical properties and digestion fate of Pickering emulsions was investigated.
33512160	3	82	theme	Pickering	685:693	arg1	emulsions					695:703	food Pickering emulsions	680:703	food Pickering emulsions for delivery of lipophilic nutraceuticals	680:745	It is the first time that three different types of emulsifiers have been used to synergistically stabilize food Pickering emulsions for delivery of lipophilic nutraceuticals.
33512160	4	83	theme	emulsions	872:880	arg1	microstructure					779:792	microstructure	779:792	microstructure	779:792	The physicochemical stability, microstructure, rheological properties, and in vitro gastrointestinal digestion of Pickering emulsions were controlled by the addition sequence and mass ratio of multiple stabilizers, which showed the enhanced stability and delayed lipid digestion of the particle-biopolymer-surfactant-stabilized Pickering emulsions.
33512160	4	83	theme	emulsions	872:880	arg1	properties					807:816	rheological properties	795:816	rheological properties	795:816	The physicochemical stability, microstructure, rheological properties, and in vitro gastrointestinal digestion of Pickering emulsions were controlled by the addition sequence and mass ratio of multiple stabilizers, which showed the enhanced stability and delayed lipid digestion of the particle-biopolymer-surfactant-stabilized Pickering emulsions.
33512160	4	83	theme	emulsions	872:880	arg1	stability					768:776	physicochemical stability	752:776	physicochemical stability	752:776	The physicochemical stability, microstructure, rheological properties, and in vitro gastrointestinal digestion of Pickering emulsions were controlled by the addition sequence and mass ratio of multiple stabilizers, which showed the enhanced stability and delayed lipid digestion of the particle-biopolymer-surfactant-stabilized Pickering emulsions.
33512160	4	83	theme	emulsions	872:880	arg1	digestion					849:857	in vitro gastrointestinal digestion	823:857	in vitro gastrointestinal digestion	823:857	The physicochemical stability, microstructure, rheological properties, and in vitro gastrointestinal digestion of Pickering emulsions were controlled by the addition sequence and mass ratio of multiple stabilizers, which showed the enhanced stability and delayed lipid digestion of the particle-biopolymer-surfactant-stabilized Pickering emulsions.
33512160	6	84	theme	multilayer	1302:1311	arg1	deposition					1313:1322	multilayer deposition	1302:1322	multilayer deposition	1302:1322	The coexistence of ZCPs, PGA, and Rha could induce the competitive displacement, multilayer deposition, and interparticle network at the interface.
33512160	4	85	theme	in	823:824	arg1	digestion					849:857	in vitro gastrointestinal digestion	823:857	in vitro gastrointestinal digestion	823:857	The physicochemical stability, microstructure, rheological properties, and in vitro gastrointestinal digestion of Pickering emulsions were controlled by the addition sequence and mass ratio of multiple stabilizers, which showed the enhanced stability and delayed lipid digestion of the particle-biopolymer-surfactant-stabilized Pickering emulsions.
33512160	2	86	from	influence	369:377	arg1	properties					502:511	physiochemical properties	487:511	physiochemical properties	487:511	The influence of the particle-surfactant, particle-biopolymer, and particle-biopolymer-surfactant mixed interfaces on the physiochemical properties and digestion fate of Pickering emulsions was investigated.
33512160	2	86	from	influence	369:377	arg1	fate					527:530	digestion fate	517:530	digestion fate	517:530	The influence of the particle-surfactant, particle-biopolymer, and particle-biopolymer-surfactant mixed interfaces on the physiochemical properties and digestion fate of Pickering emulsions was investigated.
33512160	4	87	theme	gastrointestinal	832:847	arg1	digestion					849:857	in vitro gastrointestinal digestion	823:857	in vitro gastrointestinal digestion	823:857	The physicochemical stability, microstructure, rheological properties, and in vitro gastrointestinal digestion of Pickering emulsions were controlled by the addition sequence and mass ratio of multiple stabilizers, which showed the enhanced stability and delayed lipid digestion of the particle-biopolymer-surfactant-stabilized Pickering emulsions.
33512160	8	88	from	emulsions	1587:1595	arg1	acids					1566:1570	free fatty acids	1555:1570	free fatty acids from Pickering emulsions	1555:1595	By modulating the interfacial composition, the release rate of free fatty acids from Pickering emulsions was reduced from 19.46% to 2.83% through different mechanisms.
33512160	8	88	from	emulsions	1587:1595	arg1	rate					1547:1550	the release rate	1535:1550	the release rate of free fatty acids from Pickering emulsions	1535:1595	By modulating the interfacial composition, the release rate of free fatty acids from Pickering emulsions was reduced from 19.46% to 2.83% through different mechanisms.
33512160	1	89	theme	zein	249:252	arg1	particles					264:272	zein colloidal particles	249:272	zein colloidal particles (ZCPs)	249:279	Novel Pickering emulsions were stabilized by complex interfaces in the presence of zein colloidal particles (ZCPs), propylene glycol alginate (PGA), and rhamnolipid (Rha) for delivery of β-carotene.
33512160	1	89	theme	zein	249:252	arg1	ZCPs					275:278	ZCPs	275:278	ZCPs	275:278	Novel Pickering emulsions were stabilized by complex interfaces in the presence of zein colloidal particles (ZCPs), propylene glycol alginate (PGA), and rhamnolipid (Rha) for delivery of β-carotene.
32447434	9	0	theme	DNA	925:927	arg1	%					982:982	38.5 mol%	974:982	38.5 mol%	974:982	The genomic DNA G + C content observed for strain KC615T was 38.5 mol%.
32447434	9	0	theme	DNA	925:927	arg1	content					935:941	The genomic DNA G + C content	913:941	The genomic DNA G + C content observed for strain KC615T	913:968	The genomic DNA G + C content observed for strain KC615T was 38.5 mol%.
32447434	8	1	theme	fatty	864:868	arg1	acids					870:874	Major fatty acids	858:874	Major fatty acids	858:874	Major fatty acids were anteiso-C15:0, C20:0 and C18:0.
32447434	4	2	theme	cell-wall	563:571	arg1	peptidoglycan					573:585	The cell-wall peptidoglycan	559:585	The cell-wall peptidoglycan	559:585	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
32447434	10	3	theme	physiological	1097:1109	arg1	properties					1111:1120	differential physiological properties	1084:1120	differential physiological properties	1084:1120	Based on 16S rRNA gene similarity, DNA-DNA hybridization value, chemotaxonomic characteristics and differential physiological properties, strain KC615T is considered to represent a novel species within the genus Shimazuella, for which the name Shimazuella alba sp.
32447434	10	4	theme	Shimazuella	1229:1239	arg1	sp					1246:1247	the name Shimazuella alba sp	1220:1247	the name Shimazuella alba sp	1220:1247	Based on 16S rRNA gene similarity, DNA-DNA hybridization value, chemotaxonomic characteristics and differential physiological properties, strain KC615T is considered to represent a novel species within the genus Shimazuella, for which the name Shimazuella alba sp.
32447434	9	5	theme	strain	956:961	arg1	KC615T					963:968	strain KC615T	956:968	strain KC615T	956:968	The genomic DNA G + C content observed for strain KC615T was 38.5 mol%.
32447434	10	6	theme	differential	1084:1095	arg1	properties					1111:1120	differential physiological properties	1084:1120	differential physiological properties	1084:1120	Based on 16S rRNA gene similarity, DNA-DNA hybridization value, chemotaxonomic characteristics and differential physiological properties, strain KC615T is considered to represent a novel species within the genus Shimazuella, for which the name Shimazuella alba sp.
32447434	3	7	theme	strain	526:531	arg1	affiliation					507:517	the affiliation	503:517	the affiliation of the strain to the genus Shimazuella	503:556	Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence showed that isolate KC615T formed a monophyletic clade with Shimazuella kribbensis KCTC 9933T, sharing 98.2% similarity and polyphasic taxonomic studies confirmed the affiliation of the strain to the genus Shimazuella.
32447434	5	8	contain	contained	647:655	arg1	hydrolysates					634:645	Whole-cell hydrolysates	623:645	Whole-cell hydrolysates	623:645	Whole-cell hydrolysates contained ribose and glucose.
32447434	5	8	contain	contained	647:655	arg2	glucose					668:674	glucose	668:674	glucose	668:674	Whole-cell hydrolysates contained ribose and glucose.
32447434	5	8	contain	contained	647:655	arg2	ribose					657:662	ribose	657:662	ribose	657:662	Whole-cell hydrolysates contained ribose and glucose.
32447434	12	9	theme	type	1272:1275	arg1	KC615T					1287:1292	KC615T	1287:1292	KC615T (= JCM 33532T = CGMCC 4.7616T)	1287:1323	The type strain is KC615T (= JCM 33532T = CGMCC 4.7616T).
32447434	12	9	theme	type	1272:1275	arg1	strain					1277:1282	The type strain	1268:1282	The type strain	1268:1282	The type strain is KC615T (= JCM 33532T = CGMCC 4.7616T).
32447434	3	10	theme	rRNA	321:324	arg1	sequence					331:338	an almost-complete 16S rRNA gene sequence	298:338	an almost-complete 16S rRNA gene sequence	298:338	Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence showed that isolate KC615T formed a monophyletic clade with Shimazuella kribbensis KCTC 9933T, sharing 98.2% similarity and polyphasic taxonomic studies confirmed the affiliation of the strain to the genus Shimazuella.
32447434	2	11	theme	novel	121:125	arg1	bacterium					148:156	A novel, Gram-stain-positive bacterium	119:156	A novel, Gram-stain-positive bacterium	119:156	A novel, Gram-stain-positive bacterium, designated KC615T, was isolated from desert soil which was collected from the Karakum Desert, Turkmenistan.
32447434	5	12	theme	Whole-cell	623:632	arg1	hydrolysates					634:645	Whole-cell hydrolysates	623:645	Whole-cell hydrolysates	623:645	Whole-cell hydrolysates contained ribose and glucose.
32447434	1	13	theme	emended	56:62	arg1	description					64:74	emended description	56:74	emended description of the genus	56:87	nov. isolated from desert soil and emended description of the genus Shimazuella Park et al. 2007.
32447434	12	14	theme	33532T = CGMCC	1301:1314	arg1	KC615T					1287:1292	KC615T	1287:1292	KC615T (= JCM 33532T = CGMCC 4.7616T)	1287:1323	The type strain is KC615T (= JCM 33532T = CGMCC 4.7616T).
32447434	12	14	theme	33532T = CGMCC	1301:1314	arg1	4.7616T					1316:1322	= JCM 33532T = CGMCC 4.7616T	1295:1322	= JCM 33532T = CGMCC 4.7616T	1295:1322	The type strain is KC615T (= JCM 33532T = CGMCC 4.7616T).
32447434	3	15	theme	gene	326:329	arg1	sequence					331:338	an almost-complete 16S rRNA gene sequence	298:338	an almost-complete 16S rRNA gene sequence	298:338	Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence showed that isolate KC615T formed a monophyletic clade with Shimazuella kribbensis KCTC 9933T, sharing 98.2% similarity and polyphasic taxonomic studies confirmed the affiliation of the strain to the genus Shimazuella.
32447434	7	16	theme	predominant	796:806	arg1	menaquinones					808:819	The predominant menaquinones	792:819	The predominant menaquinones (> 10%)	792:827	The predominant menaquinones (> 10%) were MK-9(H4) and MK-10(H4).
32447434	7	16	theme	predominant	796:806	arg1	%					826:826	> 10%	822:826	> 10%	822:826	The predominant menaquinones (> 10%) were MK-9(H4) and MK-10(H4).
32447434	7	16	theme	predominant	796:806	arg1	MK-9					834:837	MK-9	834:837	MK-9	834:837	The predominant menaquinones (> 10%) were MK-9(H4) and MK-10(H4).
32447434	10	17	theme	name	1224:1227	arg1	sp					1246:1247	the name Shimazuella alba sp	1220:1247	the name Shimazuella alba sp	1220:1247	Based on 16S rRNA gene similarity, DNA-DNA hybridization value, chemotaxonomic characteristics and differential physiological properties, strain KC615T is considered to represent a novel species within the genus Shimazuella, for which the name Shimazuella alba sp.
32447434	2	18	theme	Karakum	237:243	arg1	Turkmenistan					253:264	Turkmenistan	253:264	Turkmenistan	253:264	A novel, Gram-stain-positive bacterium, designated KC615T, was isolated from desert soil which was collected from the Karakum Desert, Turkmenistan.
32447434	2	18	theme	Karakum	237:243	arg1	Desert					245:250	the Karakum Desert	233:250	the Karakum Desert	233:250	A novel, Gram-stain-positive bacterium, designated KC615T, was isolated from desert soil which was collected from the Karakum Desert, Turkmenistan.
32447434	2	19	attach	isolated	182:189	arg1	soil					203:206	desert soil	196:206	desert soil which was collected from the Karakum Desert, Turkmenistan	196:264	A novel, Gram-stain-positive bacterium, designated KC615T, was isolated from desert soil which was collected from the Karakum Desert, Turkmenistan.
32447434	2	19	attach	isolated	182:189	arg2	bacterium					148:156	A novel, Gram-stain-positive bacterium	119:156	A novel, Gram-stain-positive bacterium	119:156	A novel, Gram-stain-positive bacterium, designated KC615T, was isolated from desert soil which was collected from the Karakum Desert, Turkmenistan.
32447434	0	20	theme	alba	12:15	arg1	sp					17:18	Shimazuella alba sp	0:18	Shimazuella alba sp.	0:19	Shimazuella alba sp.
32447434	10	21	theme	strain	1123:1128	arg1	KC615T					1130:1135	strain KC615T	1123:1135	strain KC615T	1123:1135	Based on 16S rRNA gene similarity, DNA-DNA hybridization value, chemotaxonomic characteristics and differential physiological properties, strain KC615T is considered to represent a novel species within the genus Shimazuella, for which the name Shimazuella alba sp.
32447434	9	22	theme	G + C	929:933	arg1	%					982:982	38.5 mol%	974:982	38.5 mol%	974:982	The genomic DNA G + C content observed for strain KC615T was 38.5 mol%.
32447434	9	22	theme	G + C	929:933	arg1	content					935:941	The genomic DNA G + C content	913:941	The genomic DNA G + C content observed for strain KC615T	913:968	The genomic DNA G + C content observed for strain KC615T was 38.5 mol%.
32447434	3	23	theme	Shimazuella	400:410	arg1	KCTC					423:426	Shimazuella kribbensis KCTC 9933T	400:432	Shimazuella kribbensis KCTC 9933T	400:432	Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence showed that isolate KC615T formed a monophyletic clade with Shimazuella kribbensis KCTC 9933T, sharing 98.2% similarity and polyphasic taxonomic studies confirmed the affiliation of the strain to the genus Shimazuella.
32447434	0	24	theme	Shimazuella	0:10	arg1	sp					17:18	Shimazuella alba sp	0:18	Shimazuella alba sp.	0:19	Shimazuella alba sp.
32447434	10	25	theme	novel	1166:1170	arg1	species					1172:1178	a novel species	1164:1178	a novel species	1164:1178	Based on 16S rRNA gene similarity, DNA-DNA hybridization value, chemotaxonomic characteristics and differential physiological properties, strain KC615T is considered to represent a novel species within the genus Shimazuella, for which the name Shimazuella alba sp.
32447434	3	26	theme	kribbensis	412:421	arg1	KCTC					423:426	Shimazuella kribbensis KCTC 9933T	400:432	Shimazuella kribbensis KCTC 9933T	400:432	Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence showed that isolate KC615T formed a monophyletic clade with Shimazuella kribbensis KCTC 9933T, sharing 98.2% similarity and polyphasic taxonomic studies confirmed the affiliation of the strain to the genus Shimazuella.
32447434	10	27	theme	alba	1241:1244	arg1	sp					1246:1247	the name Shimazuella alba sp	1220:1247	the name Shimazuella alba sp	1220:1247	Based on 16S rRNA gene similarity, DNA-DNA hybridization value, chemotaxonomic characteristics and differential physiological properties, strain KC615T is considered to represent a novel species within the genus Shimazuella, for which the name Shimazuella alba sp.
32447434	1	28	theme	genus	83:87	arg1	soil					47:50	desert soil	40:50	desert soil	40:50	nov. isolated from desert soil and emended description of the genus Shimazuella Park et al. 2007.
32447434	1	28	theme	genus	83:87	arg1	description					64:74	emended description	56:74	emended description of the genus	56:87	nov. isolated from desert soil and emended description of the genus Shimazuella Park et al. 2007.
32447434	3	29	theme	98.2	443:446	arg1	%					447:447	%	447:447	%	447:447	Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence showed that isolate KC615T formed a monophyletic clade with Shimazuella kribbensis KCTC 9933T, sharing 98.2% similarity and polyphasic taxonomic studies confirmed the affiliation of the strain to the genus Shimazuella.
32447434	1	30	dep	Park	101:104	arg1	al.					109:111	Shimazuella Park et al.	89:111	Shimazuella Park et al.	89:111	nov. isolated from desert soil and emended description of the genus Shimazuella Park et al. 2007.
32447434	3	31	theme	genus	540:544	arg1	Shimazuella					546:556	the genus Shimazuella	536:556	the genus Shimazuella	536:556	Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence showed that isolate KC615T formed a monophyletic clade with Shimazuella kribbensis KCTC 9933T, sharing 98.2% similarity and polyphasic taxonomic studies confirmed the affiliation of the strain to the genus Shimazuella.
32447434	3	32	theme	%	447:447	arg1	similarity					449:458	98.2% similarity	443:458	98.2% similarity	443:458	Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence showed that isolate KC615T formed a monophyletic clade with Shimazuella kribbensis KCTC 9933T, sharing 98.2% similarity and polyphasic taxonomic studies confirmed the affiliation of the strain to the genus Shimazuella.
32447434	2	33	dep	novel	121:125	arg1	Gram-stain-positive					128:146	Gram-stain-positive	128:146	Gram-stain-positive	128:146	A novel, Gram-stain-positive bacterium, designated KC615T, was isolated from desert soil which was collected from the Karakum Desert, Turkmenistan.
32447434	10	34	theme	hybridization	1028:1040	arg1	value					1042:1046	DNA-DNA hybridization value	1020:1046	DNA-DNA hybridization value	1020:1046	Based on 16S rRNA gene similarity, DNA-DNA hybridization value, chemotaxonomic characteristics and differential physiological properties, strain KC615T is considered to represent a novel species within the genus Shimazuella, for which the name Shimazuella alba sp.
32447434	6	35	theme	polar	687:691	arg1	diphosphatidylglycerol					705:726	diphosphatidylglycerol	705:726	diphosphatidylglycerol	705:726	The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and hydroxy-phosphatidylethanolamine.
32447434	6	35	theme	polar	687:691	arg1	lipids					693:698	The major polar lipids	677:698	The major polar lipids	677:698	The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and hydroxy-phosphatidylethanolamine.
32447434	10	36	theme	genus	1191:1195	arg1	Shimazuella					1197:1207	the genus Shimazuella	1187:1207	the genus Shimazuella	1187:1207	Based on 16S rRNA gene similarity, DNA-DNA hybridization value, chemotaxonomic characteristics and differential physiological properties, strain KC615T is considered to represent a novel species within the genus Shimazuella, for which the name Shimazuella alba sp.
32447434	10	37	theme	DNA-DNA	1020:1026	arg1	hybridization					1028:1040	DNA-DNA hybridization	1020:1040	DNA-DNA hybridization value	1020:1046	Based on 16S rRNA gene similarity, DNA-DNA hybridization value, chemotaxonomic characteristics and differential physiological properties, strain KC615T is considered to represent a novel species within the genus Shimazuella, for which the name Shimazuella alba sp.
32447434	4	38	contain	contained	587:595	arg1	peptidoglycan					573:585	The cell-wall peptidoglycan	559:585	The cell-wall peptidoglycan	559:585	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
32447434	4	38	contain	contained	587:595	arg2	acid					617:620	meso-diaminopimelic acid	597:620	meso-diaminopimelic acid	597:620	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
32447434	9	39	theme	38.5 mol	974:981	arg1	%					982:982	38.5 mol%	974:982	38.5 mol%	974:982	The genomic DNA G + C content observed for strain KC615T was 38.5 mol%.
32447434	9	39	theme	38.5 mol	974:981	arg1	content					935:941	The genomic DNA G + C content	913:941	The genomic DNA G + C content observed for strain KC615T	913:968	The genomic DNA G + C content observed for strain KC615T was 38.5 mol%.
32447434	2	40	theme	desert	196:201	arg1	soil					203:206	desert soil	196:206	desert soil which was collected from the Karakum Desert, Turkmenistan	196:264	A novel, Gram-stain-positive bacterium, designated KC615T, was isolated from desert soil which was collected from the Karakum Desert, Turkmenistan.
32447434	3	41	theme	polyphasic	464:473	arg1	studies					485:491	polyphasic taxonomic studies	464:491	polyphasic taxonomic studies	464:491	Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence showed that isolate KC615T formed a monophyletic clade with Shimazuella kribbensis KCTC 9933T, sharing 98.2% similarity and polyphasic taxonomic studies confirmed the affiliation of the strain to the genus Shimazuella.
32447434	3	42	theme	monophyletic	376:387	arg1	clade					389:393	a monophyletic clade	374:393	a monophyletic clade	374:393	Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence showed that isolate KC615T formed a monophyletic clade with Shimazuella kribbensis KCTC 9933T, sharing 98.2% similarity and polyphasic taxonomic studies confirmed the affiliation of the strain to the genus Shimazuella.
32447434	3	43	theme	taxonomic	475:483	arg1	studies					485:491	polyphasic taxonomic studies	464:491	polyphasic taxonomic studies	464:491	Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence showed that isolate KC615T formed a monophyletic clade with Shimazuella kribbensis KCTC 9933T, sharing 98.2% similarity and polyphasic taxonomic studies confirmed the affiliation of the strain to the genus Shimazuella.
32447434	6	44	theme	major	681:685	arg1	diphosphatidylglycerol					705:726	diphosphatidylglycerol	705:726	diphosphatidylglycerol	705:726	The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and hydroxy-phosphatidylethanolamine.
32447434	6	44	theme	major	681:685	arg1	lipids					693:698	The major polar lipids	677:698	The major polar lipids	677:698	The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and hydroxy-phosphatidylethanolamine.
32447434	10	45	theme	16S	994:996	arg1	similarity					1008:1017	16S rRNA gene similarity	994:1017	16S rRNA gene similarity	994:1017	Based on 16S rRNA gene similarity, DNA-DNA hybridization value, chemotaxonomic characteristics and differential physiological properties, strain KC615T is considered to represent a novel species within the genus Shimazuella, for which the name Shimazuella alba sp.
32447434	9	46	theme	genomic	917:923	arg1	%					982:982	38.5 mol%	974:982	38.5 mol%	974:982	The genomic DNA G + C content observed for strain KC615T was 38.5 mol%.
32447434	9	46	theme	genomic	917:923	arg1	content					935:941	The genomic DNA G + C content	913:941	The genomic DNA G + C content observed for strain KC615T	913:968	The genomic DNA G + C content observed for strain KC615T was 38.5 mol%.
32447434	1	47	dep	soil	47:50	arg1	Park					101:104	Park	101:104	Park	101:104	nov. isolated from desert soil and emended description of the genus Shimazuella Park et al. 2007.
32447434	3	48	theme	Phylogenetic	267:278	arg1	analysis					280:287	Phylogenetic analysis	267:287	Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence	267:338	Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence showed that isolate KC615T formed a monophyletic clade with Shimazuella kribbensis KCTC 9933T, sharing 98.2% similarity and polyphasic taxonomic studies confirmed the affiliation of the strain to the genus Shimazuella.
32447434	10	49	theme	chemotaxonomic	1049:1062	arg1	characteristics					1064:1078	chemotaxonomic characteristics	1049:1078	chemotaxonomic characteristics	1049:1078	Based on 16S rRNA gene similarity, DNA-DNA hybridization value, chemotaxonomic characteristics and differential physiological properties, strain KC615T is considered to represent a novel species within the genus Shimazuella, for which the name Shimazuella alba sp.
32447434	10	50	theme	gene	1003:1006	arg1	similarity					1008:1017	16S rRNA gene similarity	994:1017	16S rRNA gene similarity	994:1017	Based on 16S rRNA gene similarity, DNA-DNA hybridization value, chemotaxonomic characteristics and differential physiological properties, strain KC615T is considered to represent a novel species within the genus Shimazuella, for which the name Shimazuella alba sp.
32447434	8	51	theme	Major	858:862	arg1	acids					870:874	Major fatty acids	858:874	Major fatty acids	858:874	Major fatty acids were anteiso-C15:0, C20:0 and C18:0.
32447434	4	52	theme	meso-diaminopimelic	597:615	arg1	acid					617:620	meso-diaminopimelic acid	597:620	meso-diaminopimelic acid	597:620	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
32447434	3	53	theme	almost-complete	301:315	arg1	rRNA					321:324	an almost-complete 16S rRNA	298:324	an almost-complete 16S rRNA gene sequence	298:338	Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence showed that isolate KC615T formed a monophyletic clade with Shimazuella kribbensis KCTC 9933T, sharing 98.2% similarity and polyphasic taxonomic studies confirmed the affiliation of the strain to the genus Shimazuella.
32447434	10	54	theme	rRNA	998:1001	arg1	similarity					1008:1017	16S rRNA gene similarity	994:1017	16S rRNA gene similarity	994:1017	Based on 16S rRNA gene similarity, DNA-DNA hybridization value, chemotaxonomic characteristics and differential physiological properties, strain KC615T is considered to represent a novel species within the genus Shimazuella, for which the name Shimazuella alba sp.
32447434	12	55	theme	= JCM	1295:1299	arg1	KC615T					1287:1292	KC615T	1287:1292	KC615T (= JCM 33532T = CGMCC 4.7616T)	1287:1323	The type strain is KC615T (= JCM 33532T = CGMCC 4.7616T).
32447434	12	55	theme	= JCM	1295:1299	arg1	4.7616T					1316:1322	= JCM 33532T = CGMCC 4.7616T	1295:1322	= JCM 33532T = CGMCC 4.7616T	1295:1322	The type strain is KC615T (= JCM 33532T = CGMCC 4.7616T).
32447434	3	56	theme	16S	317:319	arg1	rRNA					321:324	an almost-complete 16S rRNA	298:324	an almost-complete 16S rRNA gene sequence	298:338	Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence showed that isolate KC615T formed a monophyletic clade with Shimazuella kribbensis KCTC 9933T, sharing 98.2% similarity and polyphasic taxonomic studies confirmed the affiliation of the strain to the genus Shimazuella.
32447434	1	57	theme	desert	40:45	arg1	soil					47:50	desert soil	40:50	desert soil	40:50	nov. isolated from desert soil and emended description of the genus Shimazuella Park et al. 2007.
32228775	3	0	theme	yellow-pigmented	288:303	arg1	bacterium					340:348	a Gram stain-negative, catalase- and oxidase-positive, aerobic, yellow-pigmented, motile by gliding, and rod-shaped bacterium	224:348	a Gram stain-negative, catalase- and oxidase-positive, aerobic, yellow-pigmented, motile by gliding, and rod-shaped bacterium	224:348	Strain PS13T was a Gram stain-negative, catalase- and oxidase-positive, aerobic, yellow-pigmented, motile by gliding, and rod-shaped bacterium.
32228775	3	0	theme	yellow-pigmented	288:303	arg1	PS13T					214:218	Strain PS13T	207:218	Strain PS13T	207:218	Strain PS13T was a Gram stain-negative, catalase- and oxidase-positive, aerobic, yellow-pigmented, motile by gliding, and rod-shaped bacterium.
32228775	8	1	theme	32.2 mol	953:960	arg1	%					961:961	32.2 mol%	953:961	32.2 mol%	953:961	The genomic DNA G+C content of the strain PS13T was 32.2 mol%.
32228775	8	1	theme	32.2 mol	953:960	arg1	content					921:927	The genomic DNA G+C content	901:927	The genomic DNA G+C content of the strain PS13T	901:947	The genomic DNA G+C content of the strain PS13T was 32.2 mol%.
32228775	8	2	theme	strain	936:941	arg1	PS13T					943:947	the strain PS13T	932:947	the strain PS13T	932:947	The genomic DNA G+C content of the strain PS13T was 32.2 mol%.
32228775	8	3	theme	genomic	905:911	arg1	%					961:961	32.2 mol%	953:961	32.2 mol%	953:961	The genomic DNA G+C content of the strain PS13T was 32.2 mol%.
32228775	8	3	theme	genomic	905:911	arg1	content					921:927	The genomic DNA G+C content	901:927	The genomic DNA G+C content of the strain PS13T	901:947	The genomic DNA G+C content of the strain PS13T was 32.2 mol%.
32228775	6	4	theme	Formosa	809:815	arg1	species					788:794	a distinct species	777:794	a distinct species of the genus Formosa	777:815	The DNA-DNA relatedness (17.3-21.8 %) and average nucleotide identity (83.6-84.6 %) values clearly indicated that strain PS13T represents a distinct species of the genus Formosa.
32228775	3	5	theme	Strain	207:212	arg1	bacterium					340:348	a Gram stain-negative, catalase- and oxidase-positive, aerobic, yellow-pigmented, motile by gliding, and rod-shaped bacterium	224:348	a Gram stain-negative, catalase- and oxidase-positive, aerobic, yellow-pigmented, motile by gliding, and rod-shaped bacterium	224:348	Strain PS13T was a Gram stain-negative, catalase- and oxidase-positive, aerobic, yellow-pigmented, motile by gliding, and rod-shaped bacterium.
32228775	3	5	theme	Strain	207:212	arg1	PS13T					214:218	Strain PS13T	207:218	Strain PS13T	207:218	Strain PS13T was a Gram stain-negative, catalase- and oxidase-positive, aerobic, yellow-pigmented, motile by gliding, and rod-shaped bacterium.
32228775	7	6	theme	 0	850:851	arg1	C15 					845:848	C15 	845:848	C15 	845:848	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	6	theme	 0	850:851	arg1	ω6c/C16 					866:873	 1 ω6c/C16 	863:873	 1 ω6c/C16 	863:873	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	6	theme	 0	850:851	arg1	iso					853:855	 0 iso	850:855	 0 iso	850:855	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	6	theme	 0	850:851	arg1	iso					894:896	iso	894:896	iso	894:896	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	6	theme	 0	850:851	arg1	ω7c					878:880	ω7c	878:880	ω7c	878:880	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	6	theme	 0	850:851	arg1	acids					834:838	The major fatty acids	818:838	The major fatty acids	818:838	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	6	theme	 0	850:851	arg1	C16 					858:861	C16 	858:861	C16 	858:861	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	9	7	theme	novel	1099:1103	arg1	species					1105:1111	a novel species	1097:1111	a novel species	1097:1111	On the basis of polyphasic characteristics, it is suggested that strain PS13T be assigned to the genus Formosa as the type strain of a novel species, for which the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp.
32228775	5	8	theme	%	615:615	arg1	similarity					626:635	98.3 % sequence similarity	610:635	98.3 % sequence similarity	610:635	Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain PS13T belonged to the genus Formosa and was closely related to Formosa algae KMM3553T (98.3 % sequence similarity).
32228775	5	8	theme	%	615:615	arg1	KMM3553T					600:607	Formosa algae KMM3553T	586:607	Formosa algae KMM3553T (98.3 % sequence similarity)	586:636	Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain PS13T belonged to the genus Formosa and was closely related to Formosa algae KMM3553T (98.3 % sequence similarity).
32228775	6	9	theme	genus	803:807	arg1	Formosa					809:815	the genus Formosa	799:815	the genus Formosa	799:815	The DNA-DNA relatedness (17.3-21.8 %) and average nucleotide identity (83.6-84.6 %) values clearly indicated that strain PS13T represents a distinct species of the genus Formosa.
32228775	3	10	theme	rod-shaped	329:338	arg1	bacterium					340:348	a Gram stain-negative, catalase- and oxidase-positive, aerobic, yellow-pigmented, motile by gliding, and rod-shaped bacterium	224:348	a Gram stain-negative, catalase- and oxidase-positive, aerobic, yellow-pigmented, motile by gliding, and rod-shaped bacterium	224:348	Strain PS13T was a Gram stain-negative, catalase- and oxidase-positive, aerobic, yellow-pigmented, motile by gliding, and rod-shaped bacterium.
32228775	3	10	theme	rod-shaped	329:338	arg1	PS13T					214:218	Strain PS13T	207:218	Strain PS13T	207:218	Strain PS13T was a Gram stain-negative, catalase- and oxidase-positive, aerobic, yellow-pigmented, motile by gliding, and rod-shaped bacterium.
32228775	4	11	theme	%	424:424	arg1	NaCl					432:435	3 % (w/v) NaCl	422:435	3 % (w/v) NaCl	422:435	Strain PS13T grew optimally at 25 °C and pH 8.0 and in the presence of 3 % (w/v) NaCl.
32228775	5	12	theme	Formosa	586:592	arg1	similarity					626:635	98.3 % sequence similarity	610:635	98.3 % sequence similarity	610:635	Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain PS13T belonged to the genus Formosa and was closely related to Formosa algae KMM3553T (98.3 % sequence similarity).
32228775	5	12	theme	Formosa	586:592	arg1	KMM3553T					600:607	Formosa algae KMM3553T	586:607	Formosa algae KMM3553T (98.3 % sequence similarity)	586:636	Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain PS13T belonged to the genus Formosa and was closely related to Formosa algae KMM3553T (98.3 % sequence similarity).
32228775	9	13	theme	strain	1029:1034	arg1	strain					1087:1092	the type strain	1078:1092	the type strain of a novel species, for which the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp	1078:1184	On the basis of polyphasic characteristics, it is suggested that strain PS13T be assigned to the genus Formosa as the type strain of a novel species, for which the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp.
32228775	9	13	theme	strain	1029:1034	arg1	PS13T					1036:1040	strain PS13T	1029:1040	strain PS13T	1029:1040	On the basis of polyphasic characteristics, it is suggested that strain PS13T be assigned to the genus Formosa as the type strain of a novel species, for which the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp.
32228775	4	14	theme	3 	422:423	arg1	%					424:424	%	424:424	%	424:424	Strain PS13T grew optimally at 25 °C and pH 8.0 and in the presence of 3 % (w/v) NaCl.
32228775	5	15	theme	genus	545:549	arg1	Formosa					551:557	the genus Formosa	541:557	the genus Formosa	541:557	Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain PS13T belonged to the genus Formosa and was closely related to Formosa algae KMM3553T (98.3 % sequence similarity).
32228775	9	16	theme	species	1105:1111	arg1	strain					1087:1092	the type strain	1078:1092	the type strain of a novel species, for which the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp	1078:1184	On the basis of polyphasic characteristics, it is suggested that strain PS13T be assigned to the genus Formosa as the type strain of a novel species, for which the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp.
32228775	9	16	theme	species	1105:1111	arg1	PS13T					1036:1040	strain PS13T	1029:1040	strain PS13T	1029:1040	On the basis of polyphasic characteristics, it is suggested that strain PS13T be assigned to the genus Formosa as the type strain of a novel species, for which the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp.
32228775	6	17	theme	identity	700:707	arg1	values					723:728	The DNA-DNA relatedness (17.3-21.8 %) and average nucleotide identity (83.6-84.6 %) values	639:728	The DNA-DNA relatedness (17.3-21.8 %) and average nucleotide identity (83.6-84.6 %) values	639:728	The DNA-DNA relatedness (17.3-21.8 %) and average nucleotide identity (83.6-84.6 %) values clearly indicated that strain PS13T represents a distinct species of the genus Formosa.
32228775	1	18	theme	marine	71:76	arg1	sediment					78:85	marine sediment	71:85	marine sediment	71:85	nov., a starch-degrading bacterium isolated from marine sediment.
32228775	0	19	theme	sediminum	8:16	arg1	sp					18:19	Formosa sediminum sp	0:19	Formosa sediminum sp.	0:20	Formosa sediminum sp.
32228775	6	20	theme	distinct	779:786	arg1	species					788:794	a distinct species	777:794	a distinct species of the genus Formosa	777:815	The DNA-DNA relatedness (17.3-21.8 %) and average nucleotide identity (83.6-84.6 %) values clearly indicated that strain PS13T represents a distinct species of the genus Formosa.
32228775	6	21	theme	nucleotide	689:698	arg1	identity					700:707	average nucleotide identity	681:707	average nucleotide identity (83.6-84.6 %)	681:721	The DNA-DNA relatedness (17.3-21.8 %) and average nucleotide identity (83.6-84.6 %) values clearly indicated that strain PS13T represents a distinct species of the genus Formosa.
32228775	6	21	theme	nucleotide	689:698	arg1	%					720:720	83.6-84.6 %	710:720	83.6-84.6 %	710:720	The DNA-DNA relatedness (17.3-21.8 %) and average nucleotide identity (83.6-84.6 %) values clearly indicated that strain PS13T represents a distinct species of the genus Formosa.
32228775	8	22	theme	DNA	913:915	arg1	%					961:961	32.2 mol%	953:961	32.2 mol%	953:961	The genomic DNA G+C content of the strain PS13T was 32.2 mol%.
32228775	8	22	theme	DNA	913:915	arg1	content					921:927	The genomic DNA G+C content	901:927	The genomic DNA G+C content of the strain PS13T	901:947	The genomic DNA G+C content of the strain PS13T was 32.2 mol%.
32228775	0	23	theme	Formosa	0:6	arg1	sp					18:19	Formosa sediminum sp	0:19	Formosa sediminum sp.	0:20	Formosa sediminum sp.
32228775	6	24	theme	average	681:687	arg1	identity					700:707	average nucleotide identity	681:707	average nucleotide identity (83.6-84.6 %)	681:721	The DNA-DNA relatedness (17.3-21.8 %) and average nucleotide identity (83.6-84.6 %) values clearly indicated that strain PS13T represents a distinct species of the genus Formosa.
32228775	6	24	theme	average	681:687	arg1	%					720:720	83.6-84.6 %	710:720	83.6-84.6 %	710:720	The DNA-DNA relatedness (17.3-21.8 %) and average nucleotide identity (83.6-84.6 %) values clearly indicated that strain PS13T represents a distinct species of the genus Formosa.
32228775	7	25	theme	fatty	828:832	arg1	C15 					845:848	C15 	845:848	C15 	845:848	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	25	theme	fatty	828:832	arg1	ω6c/C16 					866:873	 1 ω6c/C16 	863:873	 1 ω6c/C16 	863:873	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	25	theme	fatty	828:832	arg1	iso					853:855	 0 iso	850:855	 0 iso	850:855	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	25	theme	fatty	828:832	arg1	iso					894:896	iso	894:896	iso	894:896	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	25	theme	fatty	828:832	arg1	ω7c					878:880	ω7c	878:880	ω7c	878:880	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	25	theme	fatty	828:832	arg1	acids					834:838	The major fatty acids	818:838	The major fatty acids	818:838	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	25	theme	fatty	828:832	arg1	C16 					858:861	C16 	858:861	C16 	858:861	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	26	theme	major	822:826	arg1	C15 					845:848	C15 	845:848	C15 	845:848	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	26	theme	major	822:826	arg1	ω6c/C16 					866:873	 1 ω6c/C16 	863:873	 1 ω6c/C16 	863:873	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	26	theme	major	822:826	arg1	iso					853:855	 0 iso	850:855	 0 iso	850:855	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	26	theme	major	822:826	arg1	iso					894:896	iso	894:896	iso	894:896	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	26	theme	major	822:826	arg1	ω7c					878:880	ω7c	878:880	ω7c	878:880	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	26	theme	major	822:826	arg1	acids					834:838	The major fatty acids	818:838	The major fatty acids	818:838	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	26	theme	major	822:826	arg1	C16 					858:861	C16 	858:861	C16 	858:861	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	3	27	theme	catalase-	247:255	arg1	bacterium					340:348	a Gram stain-negative, catalase- and oxidase-positive, aerobic, yellow-pigmented, motile by gliding, and rod-shaped bacterium	224:348	a Gram stain-negative, catalase- and oxidase-positive, aerobic, yellow-pigmented, motile by gliding, and rod-shaped bacterium	224:348	Strain PS13T was a Gram stain-negative, catalase- and oxidase-positive, aerobic, yellow-pigmented, motile by gliding, and rod-shaped bacterium.
32228775	3	27	theme	catalase-	247:255	arg1	PS13T					214:218	Strain PS13T	207:218	Strain PS13T	207:218	Strain PS13T was a Gram stain-negative, catalase- and oxidase-positive, aerobic, yellow-pigmented, motile by gliding, and rod-shaped bacterium.
32228775	5	28	theme	strain	516:521	arg1	PS13T					523:527	strain PS13T	516:527	strain PS13T	516:527	Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain PS13T belonged to the genus Formosa and was closely related to Formosa algae KMM3553T (98.3 % sequence similarity).
32228775	9	29	theme	polyphasic	980:989	arg1	characteristics					991:1005	polyphasic characteristics	980:1005	polyphasic characteristics	980:1005	On the basis of polyphasic characteristics, it is suggested that strain PS13T be assigned to the genus Formosa as the type strain of a novel species, for which the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp.
32228775	6	30	theme	DNA-DNA	643:649	arg1	relatedness					651:661	DNA-DNA relatedness	643:661	DNA-DNA relatedness (17.3-21.8 %)	643:675	The DNA-DNA relatedness (17.3-21.8 %) and average nucleotide identity (83.6-84.6 %) values clearly indicated that strain PS13T represents a distinct species of the genus Formosa.
32228775	6	30	theme	DNA-DNA	643:649	arg1	%					674:674	17.3-21.8 %	664:674	17.3-21.8 %	664:674	The DNA-DNA relatedness (17.3-21.8 %) and average nucleotide identity (83.6-84.6 %) values clearly indicated that strain PS13T represents a distinct species of the genus Formosa.
32228775	4	31	dep	%	424:424	arg1	w/v					427:429	w/v	427:429	w/v	427:429	Strain PS13T grew optimally at 25 °C and pH 8.0 and in the presence of 3 % (w/v) NaCl.
32228775	5	32	theme	sequence	617:624	arg1	similarity					626:635	98.3 % sequence similarity	610:635	98.3 % sequence similarity	610:635	Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain PS13T belonged to the genus Formosa and was closely related to Formosa algae KMM3553T (98.3 % sequence similarity).
32228775	5	32	theme	sequence	617:624	arg1	KMM3553T					600:607	Formosa algae KMM3553T	586:607	Formosa algae KMM3553T (98.3 % sequence similarity)	586:636	Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain PS13T belonged to the genus Formosa and was closely related to Formosa algae KMM3553T (98.3 % sequence similarity).
32228775	9	33	theme	name	1128:1131	arg1	9918T					1176:1180	=KCCM 43301T=CECT 9918T	1158:1180	=KCCM 43301T=CECT 9918T	1158:1180	On the basis of polyphasic characteristics, it is suggested that strain PS13T be assigned to the genus Formosa as the type strain of a novel species, for which the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp.
32228775	9	33	theme	name	1128:1131	arg1	PS13T					1151:1155	name Formosa sediminum PS13T	1128:1155	the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp	1124:1184	On the basis of polyphasic characteristics, it is suggested that strain PS13T be assigned to the genus Formosa as the type strain of a novel species, for which the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp.
32228775	3	34	theme	stain-negative	231:244	arg1	bacterium					340:348	a Gram stain-negative, catalase- and oxidase-positive, aerobic, yellow-pigmented, motile by gliding, and rod-shaped bacterium	224:348	a Gram stain-negative, catalase- and oxidase-positive, aerobic, yellow-pigmented, motile by gliding, and rod-shaped bacterium	224:348	Strain PS13T was a Gram stain-negative, catalase- and oxidase-positive, aerobic, yellow-pigmented, motile by gliding, and rod-shaped bacterium.
32228775	3	34	theme	stain-negative	231:244	arg1	PS13T					214:218	Strain PS13T	207:218	Strain PS13T	207:218	Strain PS13T was a Gram stain-negative, catalase- and oxidase-positive, aerobic, yellow-pigmented, motile by gliding, and rod-shaped bacterium.
32228775	9	35	theme	type	1082:1085	arg1	strain					1087:1092	the type strain	1078:1092	the type strain of a novel species, for which the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp	1078:1184	On the basis of polyphasic characteristics, it is suggested that strain PS13T be assigned to the genus Formosa as the type strain of a novel species, for which the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp.
32228775	9	35	theme	type	1082:1085	arg1	PS13T					1036:1040	strain PS13T	1029:1040	strain PS13T	1029:1040	On the basis of polyphasic characteristics, it is suggested that strain PS13T be assigned to the genus Formosa as the type strain of a novel species, for which the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp.
32228775	9	36	dep	characteristics	991:1005	arg1	the					967:969	the	967:969	the	967:969	On the basis of polyphasic characteristics, it is suggested that strain PS13T be assigned to the genus Formosa as the type strain of a novel species, for which the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp.
32228775	9	36	dep	characteristics	991:1005	arg1	basis					971:975	basis	971:975	basis	971:975	On the basis of polyphasic characteristics, it is suggested that strain PS13T be assigned to the genus Formosa as the type strain of a novel species, for which the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp.
32228775	2	37	theme	novel	90:94	arg1	bacterium					96:104	A novel bacterium	88:104	A novel bacterium	88:104	A novel bacterium, designated strain PS13T, was isolated from marine sediment collected from the coast of Jeju Island.
32228775	8	38	theme	G+C	917:919	arg1	%					961:961	32.2 mol%	953:961	32.2 mol%	953:961	The genomic DNA G+C content of the strain PS13T was 32.2 mol%.
32228775	8	38	theme	G+C	917:919	arg1	content					921:927	The genomic DNA G+C content	901:927	The genomic DNA G+C content of the strain PS13T	901:947	The genomic DNA G+C content of the strain PS13T was 32.2 mol%.
32228775	2	39	theme	marine	150:155	arg1	sediment					157:164	marine sediment	150:164	marine sediment collected from the coast of Jeju Island	150:204	A novel bacterium, designated strain PS13T, was isolated from marine sediment collected from the coast of Jeju Island.
32228775	5	40	dep	Formosa	586:592	arg1	algae					594:598	algae	594:598	algae	594:598	Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain PS13T belonged to the genus Formosa and was closely related to Formosa algae KMM3553T (98.3 % sequence similarity).
32228775	9	41	theme	Formosa	1133:1139	arg1	9918T					1176:1180	=KCCM 43301T=CECT 9918T	1158:1180	=KCCM 43301T=CECT 9918T	1158:1180	On the basis of polyphasic characteristics, it is suggested that strain PS13T be assigned to the genus Formosa as the type strain of a novel species, for which the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp.
32228775	9	41	theme	Formosa	1133:1139	arg1	PS13T					1151:1155	name Formosa sediminum PS13T	1128:1155	the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp	1124:1184	On the basis of polyphasic characteristics, it is suggested that strain PS13T be assigned to the genus Formosa as the type strain of a novel species, for which the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp.
32228775	3	42	theme	aerobic	279:285	arg1	bacterium					340:348	a Gram stain-negative, catalase- and oxidase-positive, aerobic, yellow-pigmented, motile by gliding, and rod-shaped bacterium	224:348	a Gram stain-negative, catalase- and oxidase-positive, aerobic, yellow-pigmented, motile by gliding, and rod-shaped bacterium	224:348	Strain PS13T was a Gram stain-negative, catalase- and oxidase-positive, aerobic, yellow-pigmented, motile by gliding, and rod-shaped bacterium.
32228775	3	42	theme	aerobic	279:285	arg1	PS13T					214:218	Strain PS13T	207:218	Strain PS13T	207:218	Strain PS13T was a Gram stain-negative, catalase- and oxidase-positive, aerobic, yellow-pigmented, motile by gliding, and rod-shaped bacterium.
32228775	6	43	theme	strain	753:758	arg1	PS13T					760:764	strain PS13T	753:764	strain PS13T	753:764	The DNA-DNA relatedness (17.3-21.8 %) and average nucleotide identity (83.6-84.6 %) values clearly indicated that strain PS13T represents a distinct species of the genus Formosa.
32228775	2	44	theme	Island	199:204	arg1	coast					185:189	the coast	181:189	the coast of Jeju Island	181:204	A novel bacterium, designated strain PS13T, was isolated from marine sediment collected from the coast of Jeju Island.
32228775	5	45	theme	16S	480:482	arg1	sequences					494:502	16S rRNA gene sequences	480:502	16S rRNA gene sequences	480:502	Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain PS13T belonged to the genus Formosa and was closely related to Formosa algae KMM3553T (98.3 % sequence similarity).
32228775	9	46	theme	PS13T	1151:1155	arg1	sp					1183:1184	the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp	1124:1184	the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp	1124:1184	On the basis of polyphasic characteristics, it is suggested that strain PS13T be assigned to the genus Formosa as the type strain of a novel species, for which the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp.
32228775	2	47	theme	strain	118:123	arg1	PS13T					125:129	strain PS13T	118:129	strain PS13T	118:129	A novel bacterium, designated strain PS13T, was isolated from marine sediment collected from the coast of Jeju Island.
32228775	2	48	theme	Jeju	194:197	arg1	Island					199:204	Jeju Island	194:204	Jeju Island	194:204	A novel bacterium, designated strain PS13T, was isolated from marine sediment collected from the coast of Jeju Island.
32228775	5	49	theme	rRNA	484:487	arg1	sequences					494:502	16S rRNA gene sequences	480:502	16S rRNA gene sequences	480:502	Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain PS13T belonged to the genus Formosa and was closely related to Formosa algae KMM3553T (98.3 % sequence similarity).
32228775	2	50	attach	isolated	136:143	arg1	sediment					157:164	marine sediment	150:164	marine sediment collected from the coast of Jeju Island	150:204	A novel bacterium, designated strain PS13T, was isolated from marine sediment collected from the coast of Jeju Island.
32228775	2	50	attach	isolated	136:143	arg2	bacterium					96:104	A novel bacterium	88:104	A novel bacterium	88:104	A novel bacterium, designated strain PS13T, was isolated from marine sediment collected from the coast of Jeju Island.
32228775	7	51	dep	C15 	845:848	arg1	C15 					845:848	C15 	845:848	C15 	845:848	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	51	dep	C15 	845:848	arg1	ω6c/C16 					866:873	 1 ω6c/C16 	863:873	 1 ω6c/C16 	863:873	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	51	dep	C15 	845:848	arg1	iso					853:855	 0 iso	850:855	 0 iso	850:855	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	51	dep	C15 	845:848	arg1	iso					894:896	iso	894:896	iso	894:896	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	51	dep	C15 	845:848	arg1	ω7c					878:880	ω7c	878:880	ω7c	878:880	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	51	dep	C15 	845:848	arg1	acids					834:838	The major fatty acids	818:838	The major fatty acids	818:838	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	51	dep	C15 	845:848	arg1	C16 					858:861	C16 	858:861	C16 	858:861	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	52	theme	 1	863:864	arg1	C15 					845:848	C15 	845:848	C15 	845:848	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	52	theme	 1	863:864	arg1	ω6c/C16 					866:873	 1 ω6c/C16 	863:873	 1 ω6c/C16 	863:873	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	52	theme	 1	863:864	arg1	iso					853:855	 0 iso	850:855	 0 iso	850:855	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	52	theme	 1	863:864	arg1	iso					894:896	iso	894:896	iso	894:896	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	52	theme	 1	863:864	arg1	ω7c					878:880	ω7c	878:880	ω7c	878:880	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	52	theme	 1	863:864	arg1	acids					834:838	The major fatty acids	818:838	The major fatty acids	818:838	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	7	52	theme	 1	863:864	arg1	C16 					858:861	C16 	858:861	C16 	858:861	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	8	53	theme	PS13T	943:947	arg1	%					961:961	32.2 mol%	953:961	32.2 mol%	953:961	The genomic DNA G+C content of the strain PS13T was 32.2 mol%.
32228775	8	53	theme	PS13T	943:947	arg1	content					921:927	The genomic DNA G+C content	901:927	The genomic DNA G+C content of the strain PS13T	901:947	The genomic DNA G+C content of the strain PS13T was 32.2 mol%.
32228775	9	54	theme	genus	1061:1065	arg1	Formosa					1067:1073	the genus Formosa	1057:1073	the genus Formosa	1057:1073	On the basis of polyphasic characteristics, it is suggested that strain PS13T be assigned to the genus Formosa as the type strain of a novel species, for which the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp.
32228775	5	55	theme	gene	489:492	arg1	sequences					494:502	16S rRNA gene sequences	480:502	16S rRNA gene sequences	480:502	Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain PS13T belonged to the genus Formosa and was closely related to Formosa algae KMM3553T (98.3 % sequence similarity).
32228775	9	56	theme	=KCCM	1158:1162	arg1	9918T					1176:1180	=KCCM 43301T=CECT 9918T	1158:1180	=KCCM 43301T=CECT 9918T	1158:1180	On the basis of polyphasic characteristics, it is suggested that strain PS13T be assigned to the genus Formosa as the type strain of a novel species, for which the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp.
32228775	9	56	theme	=KCCM	1158:1162	arg1	PS13T					1151:1155	name Formosa sediminum PS13T	1128:1155	the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp	1124:1184	On the basis of polyphasic characteristics, it is suggested that strain PS13T be assigned to the genus Formosa as the type strain of a novel species, for which the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp.
32228775	5	57	theme	98.3 	610:614	arg1	%					615:615	%	615:615	%	615:615	Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain PS13T belonged to the genus Formosa and was closely related to Formosa algae KMM3553T (98.3 % sequence similarity).
32228775	6	58	theme	relatedness	651:661	arg1	values					723:728	The DNA-DNA relatedness (17.3-21.8 %) and average nucleotide identity (83.6-84.6 %) values	639:728	The DNA-DNA relatedness (17.3-21.8 %) and average nucleotide identity (83.6-84.6 %) values	639:728	The DNA-DNA relatedness (17.3-21.8 %) and average nucleotide identity (83.6-84.6 %) values clearly indicated that strain PS13T represents a distinct species of the genus Formosa.
32228775	9	59	theme	43301T=CECT	1164:1174	arg1	9918T					1176:1180	=KCCM 43301T=CECT 9918T	1158:1180	=KCCM 43301T=CECT 9918T	1158:1180	On the basis of polyphasic characteristics, it is suggested that strain PS13T be assigned to the genus Formosa as the type strain of a novel species, for which the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp.
32228775	9	59	theme	43301T=CECT	1164:1174	arg1	PS13T					1151:1155	name Formosa sediminum PS13T	1128:1155	the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp	1124:1184	On the basis of polyphasic characteristics, it is suggested that strain PS13T be assigned to the genus Formosa as the type strain of a novel species, for which the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp.
32228775	3	60	theme	motile	306:311	arg1	bacterium					340:348	a Gram stain-negative, catalase- and oxidase-positive, aerobic, yellow-pigmented, motile by gliding, and rod-shaped bacterium	224:348	a Gram stain-negative, catalase- and oxidase-positive, aerobic, yellow-pigmented, motile by gliding, and rod-shaped bacterium	224:348	Strain PS13T was a Gram stain-negative, catalase- and oxidase-positive, aerobic, yellow-pigmented, motile by gliding, and rod-shaped bacterium.
32228775	3	60	theme	motile	306:311	arg1	PS13T					214:218	Strain PS13T	207:218	Strain PS13T	207:218	Strain PS13T was a Gram stain-negative, catalase- and oxidase-positive, aerobic, yellow-pigmented, motile by gliding, and rod-shaped bacterium.
32228775	5	61	theme	phylogenetic	449:460	arg1	analysis					462:469	phylogenetic analysis	449:469	phylogenetic analysis based on 16S rRNA gene sequences	449:502	Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain PS13T belonged to the genus Formosa and was closely related to Formosa algae KMM3553T (98.3 % sequence similarity).
32228775	7	62	dep	iso	853:855	arg1	G					898:898	G	898:898	G	898:898	The major fatty acids were C15 : 0 iso, C16 : 1 ω6c/C16 : 1 ω7c and C15 : 1 iso G.
32228775	4	63	theme	NaCl	432:435	arg1	presence					410:417	the presence	406:417	the presence of 3 % (w/v) NaCl	406:435	Strain PS13T grew optimally at 25 °C and pH 8.0 and in the presence of 3 % (w/v) NaCl.
32228775	1	64	theme	starch-degrading	30:45	arg1	bacterium					47:55	a starch-degrading bacterium	28:55	a starch-degrading bacterium	28:55	nov., a starch-degrading bacterium isolated from marine sediment.
32228775	1	64	theme	starch-degrading	30:45	arg1	nov.					22:25	nov.	22:25	nov.	22:25	nov., a starch-degrading bacterium isolated from marine sediment.
32228775	5	65	theme	analysis	462:469	arg1	Results					438:444	Results	438:444	Results of phylogenetic analysis based on 16S rRNA gene sequences	438:502	Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain PS13T belonged to the genus Formosa and was closely related to Formosa algae KMM3553T (98.3 % sequence similarity).
32228775	9	66	theme	sediminum	1141:1149	arg1	9918T					1176:1180	=KCCM 43301T=CECT 9918T	1158:1180	=KCCM 43301T=CECT 9918T	1158:1180	On the basis of polyphasic characteristics, it is suggested that strain PS13T be assigned to the genus Formosa as the type strain of a novel species, for which the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp.
32228775	9	66	theme	sediminum	1141:1149	arg1	PS13T					1151:1155	name Formosa sediminum PS13T	1128:1155	the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp	1124:1184	On the basis of polyphasic characteristics, it is suggested that strain PS13T be assigned to the genus Formosa as the type strain of a novel species, for which the name Formosa sediminum PS13T (=KCCM 43301T=CECT 9918T) sp.
32206967	0	0	theme	Substrates	86:95	arg1	Usage					64:68	the Usage	60:68	the Usage of Agricultural Substrates and Microorganisms	60:114	Composition of Synthesized Cellulolytic Enzymes Varied with the Usage of Agricultural Substrates and Microorganisms.
32206967	4	1	theme	Nocardiopsis	818:829	arg1	sp					831:832	Nocardiopsis sp	818:832	Nocardiopsis sp	818:832	Water hyacinth produced (U ml-1) endoglucanase (51.13) and filter paperase (0.55), and corn straw produced (U ml-1) β-glucosidase (4.65), xylanase (113.32), and glucoamylase (41.27) after 7-day incubation using Nocardiopsis sp.
32206967	8	2	theme	chrysosporium	1215:1227	arg1	co-culture					1161:1170	co-culture	1161:1170	co-culture of T. reesei, T. viride, A. flavus, and P. chrysosporium	1161:1227	Among all, co-culture of T. reesei, T. viride, A. flavus, and P. chrysosporium showed highest β-glucosidase (17.05 U ml-1).
32206967	1	3	theme	sugars	270:275	arg1	amount					251:256	high amount	246:256	high amount of reducing sugars	246:275	We evaluated various agricultural lignocellulosic biomass and variety of fungi to produce cellulolytic enzymes cocktail to yield high amount of reducing sugars.
32206967	1	3	theme	sugars	270:275	arg1	sugars					270:275	reducing sugars	261:275	reducing sugars	261:275	We evaluated various agricultural lignocellulosic biomass and variety of fungi to produce cellulolytic enzymes cocktail to yield high amount of reducing sugars.
32206967	0	4	theme	Agricultural	73:84	arg1	Substrates					86:95	Agricultural Substrates	73:95	Agricultural Substrates	73:95	Composition of Synthesized Cellulolytic Enzymes Varied with the Usage of Agricultural Substrates and Microorganisms.
32206967	6	5	theme	enzymes	867:873	arg1	Production					840:849	Production	840:849	Production of cellulolytic enzymes	840:873	Production of cellulolytic enzymes was altered due to addition of various nitrogen sources, metal ions, vitamins, and amino acids.
32206967	2	6	theme	soybean	364:370	arg1	husk/tops					372:380	soybean husk/tops	364:380	soybean husk/tops	364:380	Solid-state fermentation was performed using water hyacinth, paddy straw, corn straw, soybean husk/tops, wheat straw, and sugarcane bagasse using fungi like Nocardiopsis sp.
32206967	0	7	theme	Microorganisms	101:114	arg1	Usage					64:68	the Usage	60:68	the Usage of Agricultural Substrates and Microorganisms	60:114	Composition of Synthesized Cellulolytic Enzymes Varied with the Usage of Agricultural Substrates and Microorganisms.
32206967	4	8	theme	U ml-1	632:637	arg1	51.13					655:659	51.13	655:659	51.13	655:659	Water hyacinth produced (U ml-1) endoglucanase (51.13) and filter paperase (0.55), and corn straw produced (U ml-1) β-glucosidase (4.65), xylanase (113.32), and glucoamylase (41.27) after 7-day incubation using Nocardiopsis sp.
32206967	4	8	theme	U ml-1	632:637	arg1	endoglucanase					640:652	(U ml-1) endoglucanase	631:652	(U ml-1) endoglucanase (51.13)	631:660	Water hyacinth produced (U ml-1) endoglucanase (51.13) and filter paperase (0.55), and corn straw produced (U ml-1) β-glucosidase (4.65), xylanase (113.32), and glucoamylase (41.27) after 7-day incubation using Nocardiopsis sp.
32206967	8	9	theme	highest	1236:1242	arg1	β-glucosidase					1244:1256	highest β-glucosidase	1236:1256	highest β-glucosidase (17.05 U ml-1)	1236:1271	Among all, co-culture of T. reesei, T. viride, A. flavus, and P. chrysosporium showed highest β-glucosidase (17.05 U ml-1).
32206967	8	9	theme	highest	1236:1242	arg1	17.05 U ml-1					1259:1270	17.05 U ml-1	1259:1270	17.05 U ml-1	1259:1270	Among all, co-culture of T. reesei, T. viride, A. flavus, and P. chrysosporium showed highest β-glucosidase (17.05 U ml-1).
32206967	4	10	theme	filter	666:671	arg1	0.55					683:686	0.55	683:686	0.55	683:686	Water hyacinth produced (U ml-1) endoglucanase (51.13) and filter paperase (0.55), and corn straw produced (U ml-1) β-glucosidase (4.65), xylanase (113.32), and glucoamylase (41.27) after 7-day incubation using Nocardiopsis sp.
32206967	4	10	theme	filter	666:671	arg1	paperase					673:680	filter paperase	666:680	filter paperase (0.55)	666:687	Water hyacinth produced (U ml-1) endoglucanase (51.13) and filter paperase (0.55), and corn straw produced (U ml-1) β-glucosidase (4.65), xylanase (113.32), and glucoamylase (41.27) after 7-day incubation using Nocardiopsis sp.
32206967	6	11	theme	ions	938:941	arg1	addition					894:901	addition	894:901	addition of various nitrogen sources, metal ions, vitamins, and amino acids	894:968	Production of cellulolytic enzymes was altered due to addition of various nitrogen sources, metal ions, vitamins, and amino acids.
32206967	10	12	theme	maximum	1482:1488	arg1	sugars					1499:1504	maximum reducing sugars	1482:1504	maximum reducing sugars	1482:1504	This study revealed the dependency on substrate and microorganism to produce good quality enzyme cocktail to obtain maximum reducing sugars.
32206967	7	13	dep	viride	1111:1116	arg1	paperase					1126:1133	filter paperase	1119:1133	filter paperase; 1.57 U ml-1	1119:1146	The maximum cellulolytic enzymes were produced by P. chrysosporium (endoglucanase; 166.32 U ml-1 and exoglucanase; 12.20 U ml-1), and by T. viride (filter paperase; 1.57 U ml-1).
32206967	6	14	theme	metal	932:936	arg1	ions					938:941	metal ions	932:941	metal ions	932:941	Production of cellulolytic enzymes was altered due to addition of various nitrogen sources, metal ions, vitamins, and amino acids.
32206967	8	15	theme	viride	1189:1194	arg1	co-culture					1161:1170	co-culture	1161:1170	co-culture of T. reesei, T. viride, A. flavus, and P. chrysosporium	1161:1227	Among all, co-culture of T. reesei, T. viride, A. flavus, and P. chrysosporium showed highest β-glucosidase (17.05 U ml-1).
32206967	6	16	theme	acids	964:968	arg1	addition					894:901	addition	894:901	addition of various nitrogen sources, metal ions, vitamins, and amino acids	894:968	Production of cellulolytic enzymes was altered due to addition of various nitrogen sources, metal ions, vitamins, and amino acids.
32206967	1	17	theme	fungi	190:194	arg1	fungi					190:194	fungi	190:194	fungi	190:194	We evaluated various agricultural lignocellulosic biomass and variety of fungi to produce cellulolytic enzymes cocktail to yield high amount of reducing sugars.
32206967	1	17	theme	fungi	190:194	arg1	variety					179:185	variety	179:185	variety	179:185	We evaluated various agricultural lignocellulosic biomass and variety of fungi to produce cellulolytic enzymes cocktail to yield high amount of reducing sugars.
32206967	1	17	theme	fungi	190:194	arg1	biomass					167:173	various agricultural lignocellulosic biomass	130:173	various agricultural lignocellulosic biomass	130:173	We evaluated various agricultural lignocellulosic biomass and variety of fungi to produce cellulolytic enzymes cocktail to yield high amount of reducing sugars.
32206967	7	18	theme	filter	1119:1124	arg1	paperase					1126:1133	filter paperase	1119:1133	filter paperase; 1.57 U ml-1	1119:1146	The maximum cellulolytic enzymes were produced by P. chrysosporium (endoglucanase; 166.32 U ml-1 and exoglucanase; 12.20 U ml-1), and by T. viride (filter paperase; 1.57 U ml-1).
32206967	6	19	theme	vitamins	944:951	arg1	addition					894:901	addition	894:901	addition of various nitrogen sources, metal ions, vitamins, and amino acids	894:968	Production of cellulolytic enzymes was altered due to addition of various nitrogen sources, metal ions, vitamins, and amino acids.
32206967	6	20	theme	amino	958:962	arg1	acids					964:968	amino acids	958:968	amino acids	958:968	Production of cellulolytic enzymes was altered due to addition of various nitrogen sources, metal ions, vitamins, and amino acids.
32206967	9	21	theme	viride + P.	1328:1338	arg1	co-culture					1354:1363	T. viride + P. chrysosporium co-culture	1325:1363	T. viride + P. chrysosporium co-culture	1325:1363	The highest xylanase (1129 U ml-1) was observed in T. viride + P. chrysosporium co-culture.
32206967	7	22	theme	T.	1108:1109	arg1	viride					1111:1116	T. viride	1108:1116	T. viride (filter paperase; 1.57 U ml-1)	1108:1147	The maximum cellulolytic enzymes were produced by P. chrysosporium (endoglucanase; 166.32 U ml-1 and exoglucanase; 12.20 U ml-1), and by T. viride (filter paperase; 1.57 U ml-1).
32206967	6	23	theme	various	906:912	arg1	sources					923:929	various nitrogen sources	906:929	various nitrogen sources	906:929	Production of cellulolytic enzymes was altered due to addition of various nitrogen sources, metal ions, vitamins, and amino acids.
32206967	7	24	theme	P.	1021:1022	arg1	chrysosporium					1024:1036	P. chrysosporium	1021:1036	P. chrysosporium (endoglucanase; 166.32 U ml-1 and exoglucanase; 12.20 U ml-1)	1021:1098	The maximum cellulolytic enzymes were produced by P. chrysosporium (endoglucanase; 166.32 U ml-1 and exoglucanase; 12.20 U ml-1), and by T. viride (filter paperase; 1.57 U ml-1).
32206967	7	25	theme	cellulolytic	983:994	arg1	enzymes					996:1002	The maximum cellulolytic enzymes	971:1002	The maximum cellulolytic enzymes	971:1002	The maximum cellulolytic enzymes were produced by P. chrysosporium (endoglucanase; 166.32 U ml-1 and exoglucanase; 12.20 U ml-1), and by T. viride (filter paperase; 1.57 U ml-1).
32206967	9	26	theme	chrysosporium	1340:1352	arg1	co-culture					1354:1363	T. viride + P. chrysosporium co-culture	1325:1363	T. viride + P. chrysosporium co-culture	1325:1363	The highest xylanase (1129 U ml-1) was observed in T. viride + P. chrysosporium co-culture.
32206967	4	27	theme	7-day	795:799	arg1	incubation					801:810	7-day incubation	795:810	7-day incubation using Nocardiopsis sp	795:832	Water hyacinth produced (U ml-1) endoglucanase (51.13) and filter paperase (0.55), and corn straw produced (U ml-1) β-glucosidase (4.65), xylanase (113.32), and glucoamylase (41.27) after 7-day incubation using Nocardiopsis sp.
32206967	2	28	theme	paddy	339:343	arg1	straw					345:349	paddy straw	339:349	paddy straw	339:349	Solid-state fermentation was performed using water hyacinth, paddy straw, corn straw, soybean husk/tops, wheat straw, and sugarcane bagasse using fungi like Nocardiopsis sp.
32206967	1	29	theme	cellulolytic	207:218	arg1	enzymes					220:226	cellulolytic enzymes	207:226	cellulolytic enzymes cocktail to yield high amount of reducing sugars	207:275	We evaluated various agricultural lignocellulosic biomass and variety of fungi to produce cellulolytic enzymes cocktail to yield high amount of reducing sugars.
32206967	0	30	theme	Cellulolytic	27:38	arg1	Enzymes					40:46	Synthesized Cellulolytic Enzymes	15:46	Synthesized Cellulolytic Enzymes	15:46	Composition of Synthesized Cellulolytic Enzymes Varied with the Usage of Agricultural Substrates and Microorganisms.
32206967	4	31	theme	U ml-1	715:720	arg1	β-glucosidase					723:735	(U ml-1) β-glucosidase	714:735	(U ml-1) β-glucosidase (4.65)	714:742	Water hyacinth produced (U ml-1) endoglucanase (51.13) and filter paperase (0.55), and corn straw produced (U ml-1) β-glucosidase (4.65), xylanase (113.32), and glucoamylase (41.27) after 7-day incubation using Nocardiopsis sp.
32206967	4	31	theme	U ml-1	715:720	arg1	4.65					738:741	4.65	738:741	4.65	738:741	Water hyacinth produced (U ml-1) endoglucanase (51.13) and filter paperase (0.55), and corn straw produced (U ml-1) β-glucosidase (4.65), xylanase (113.32), and glucoamylase (41.27) after 7-day incubation using Nocardiopsis sp.
32206967	9	32	located	observed	1313:1320	arg1	co-culture					1354:1363	T. viride + P. chrysosporium co-culture	1325:1363	T. viride + P. chrysosporium co-culture	1325:1363	The highest xylanase (1129 U ml-1) was observed in T. viride + P. chrysosporium co-culture.
32206967	9	32	located	observed	1313:1320	arg2	1129 U ml-1					1296:1306	1129 U ml-1	1296:1306	1129 U ml-1	1296:1306	The highest xylanase (1129 U ml-1) was observed in T. viride + P. chrysosporium co-culture.
32206967	9	32	located	observed	1313:1320	arg2	xylanase					1286:1293	The highest xylanase	1274:1293	The highest xylanase (1129 U ml-1)	1274:1307	The highest xylanase (1129 U ml-1) was observed in T. viride + P. chrysosporium co-culture.
32206967	8	33	theme	reesei	1178:1183	arg1	co-culture					1161:1170	co-culture	1161:1170	co-culture of T. reesei, T. viride, A. flavus, and P. chrysosporium	1161:1227	Among all, co-culture of T. reesei, T. viride, A. flavus, and P. chrysosporium showed highest β-glucosidase (17.05 U ml-1).
32206967	2	34	theme	Nocardiopsis	435:446	arg1	sp					448:449	Nocardiopsis sp	435:449	Nocardiopsis sp	435:449	Solid-state fermentation was performed using water hyacinth, paddy straw, corn straw, soybean husk/tops, wheat straw, and sugarcane bagasse using fungi like Nocardiopsis sp.
32206967	6	35	theme	nitrogen	914:921	arg1	sources					923:929	various nitrogen sources	906:929	various nitrogen sources	906:929	Production of cellulolytic enzymes was altered due to addition of various nitrogen sources, metal ions, vitamins, and amino acids.
32206967	7	36	dep	endoglucanase	1039:1051	arg1	166.32 U ml-1					1054:1066	166.32 U ml-1	1054:1066	166.32 U ml-1	1054:1066	The maximum cellulolytic enzymes were produced by P. chrysosporium (endoglucanase; 166.32 U ml-1 and exoglucanase; 12.20 U ml-1), and by T. viride (filter paperase; 1.57 U ml-1).
32206967	7	36	dep	endoglucanase	1039:1051	arg1	12.20 U ml-1					1086:1097	12.20 U ml-1	1086:1097	endoglucanase; 166.32 U ml-1 and exoglucanase; 12.20 U ml-1	1039:1097	The maximum cellulolytic enzymes were produced by P. chrysosporium (endoglucanase; 166.32 U ml-1 and exoglucanase; 12.20 U ml-1), and by T. viride (filter paperase; 1.57 U ml-1).
32206967	7	36	dep	endoglucanase	1039:1051	arg1	exoglucanase					1072:1083	exoglucanase	1072:1083	exoglucanase	1072:1083	The maximum cellulolytic enzymes were produced by P. chrysosporium (endoglucanase; 166.32 U ml-1 and exoglucanase; 12.20 U ml-1), and by T. viride (filter paperase; 1.57 U ml-1).
32206967	8	37	theme	P.	1212:1213	arg1	chrysosporium					1215:1227	P. chrysosporium	1212:1227	P. chrysosporium	1212:1227	Among all, co-culture of T. reesei, T. viride, A. flavus, and P. chrysosporium showed highest β-glucosidase (17.05 U ml-1).
32206967	3	38	theme	Aspergillus	497:507	arg1	flavus					509:514	Aspergillus flavus	497:514	Aspergillus flavus	497:514	KNU, Trichoderma reesei, Trichoderma viride, Aspergillus flavus, and Phanerochaete chrysosporium alone and in combination to produce cellulolytic enzymes.
32206967	8	39	theme	flavus	1200:1205	arg1	co-culture					1161:1170	co-culture	1161:1170	co-culture of T. reesei, T. viride, A. flavus, and P. chrysosporium	1161:1227	Among all, co-culture of T. reesei, T. viride, A. flavus, and P. chrysosporium showed highest β-glucosidase (17.05 U ml-1).
32206967	3	40	theme	cellulolytic	585:596	arg1	enzymes					598:604	cellulolytic enzymes	585:604	cellulolytic enzymes	585:604	KNU, Trichoderma reesei, Trichoderma viride, Aspergillus flavus, and Phanerochaete chrysosporium alone and in combination to produce cellulolytic enzymes.
32206967	0	41	theme	Enzymes	40:46	arg1	Composition					0:10	Composition	0:10	Composition of Synthesized Cellulolytic Enzymes	0:46	Composition of Synthesized Cellulolytic Enzymes Varied with the Usage of Agricultural Substrates and Microorganisms.
32206967	7	42	dep	chrysosporium	1024:1036	arg1	endoglucanase					1039:1051	endoglucanase	1039:1051	endoglucanase; 166.32 U ml-1 and exoglucanase; 12.20 U ml-1	1039:1097	The maximum cellulolytic enzymes were produced by P. chrysosporium (endoglucanase; 166.32 U ml-1 and exoglucanase; 12.20 U ml-1), and by T. viride (filter paperase; 1.57 U ml-1).
32206967	4	43	theme	corn	694:697	arg1	straw					699:703	corn straw	694:703	corn straw	694:703	Water hyacinth produced (U ml-1) endoglucanase (51.13) and filter paperase (0.55), and corn straw produced (U ml-1) β-glucosidase (4.65), xylanase (113.32), and glucoamylase (41.27) after 7-day incubation using Nocardiopsis sp.
32206967	3	44	theme	Trichoderma	457:467	arg1	reesei					469:474	Trichoderma reesei	457:474	Trichoderma reesei	457:474	KNU, Trichoderma reesei, Trichoderma viride, Aspergillus flavus, and Phanerochaete chrysosporium alone and in combination to produce cellulolytic enzymes.
32206967	10	45	theme	reducing	1490:1497	arg1	sugars					1499:1504	maximum reducing sugars	1482:1504	maximum reducing sugars	1482:1504	This study revealed the dependency on substrate and microorganism to produce good quality enzyme cocktail to obtain maximum reducing sugars.
32206967	7	46	theme	maximum	975:981	arg1	enzymes					996:1002	The maximum cellulolytic enzymes	971:1002	The maximum cellulolytic enzymes	971:1002	The maximum cellulolytic enzymes were produced by P. chrysosporium (endoglucanase; 166.32 U ml-1 and exoglucanase; 12.20 U ml-1), and by T. viride (filter paperase; 1.57 U ml-1).
32206967	6	47	theme	sources	923:929	arg1	addition					894:901	addition	894:901	addition of various nitrogen sources, metal ions, vitamins, and amino acids	894:968	Production of cellulolytic enzymes was altered due to addition of various nitrogen sources, metal ions, vitamins, and amino acids.
32206967	9	48	theme	T.	1325:1326	arg1	co-culture					1354:1363	T. viride + P. chrysosporium co-culture	1325:1363	T. viride + P. chrysosporium co-culture	1325:1363	The highest xylanase (1129 U ml-1) was observed in T. viride + P. chrysosporium co-culture.
32206967	6	49	theme	cellulolytic	854:865	arg1	enzymes					867:873	cellulolytic enzymes	854:873	cellulolytic enzymes	854:873	Production of cellulolytic enzymes was altered due to addition of various nitrogen sources, metal ions, vitamins, and amino acids.
32206967	2	50	theme	sugarcane	400:408	arg1	bagasse					410:416	sugarcane bagasse	400:416	sugarcane bagasse	400:416	Solid-state fermentation was performed using water hyacinth, paddy straw, corn straw, soybean husk/tops, wheat straw, and sugarcane bagasse using fungi like Nocardiopsis sp.
32206967	3	51	theme	Trichoderma	477:487	arg1	viride					489:494	Trichoderma viride	477:494	Trichoderma viride	477:494	KNU, Trichoderma reesei, Trichoderma viride, Aspergillus flavus, and Phanerochaete chrysosporium alone and in combination to produce cellulolytic enzymes.
32206967	1	52	theme	various	130:136	arg1	biomass					167:173	various agricultural lignocellulosic biomass	130:173	various agricultural lignocellulosic biomass	130:173	We evaluated various agricultural lignocellulosic biomass and variety of fungi to produce cellulolytic enzymes cocktail to yield high amount of reducing sugars.
32206967	7	53	dep	paperase	1126:1133	arg1	1.57 U ml-1					1136:1146	1.57 U ml-1	1136:1146	filter paperase; 1.57 U ml-1	1119:1146	The maximum cellulolytic enzymes were produced by P. chrysosporium (endoglucanase; 166.32 U ml-1 and exoglucanase; 12.20 U ml-1), and by T. viride (filter paperase; 1.57 U ml-1).
32206967	10	54	from	dependency	1390:1399	arg1	microorganism					1418:1430	microorganism	1418:1430	microorganism	1418:1430	This study revealed the dependency on substrate and microorganism to produce good quality enzyme cocktail to obtain maximum reducing sugars.
32206967	10	54	from	dependency	1390:1399	arg1	substrate					1404:1412	substrate	1404:1412	substrate	1404:1412	This study revealed the dependency on substrate and microorganism to produce good quality enzyme cocktail to obtain maximum reducing sugars.
32206967	1	55	theme	agricultural	138:149	arg1	biomass					167:173	various agricultural lignocellulosic biomass	130:173	various agricultural lignocellulosic biomass	130:173	We evaluated various agricultural lignocellulosic biomass and variety of fungi to produce cellulolytic enzymes cocktail to yield high amount of reducing sugars.
32206967	9	56	theme	highest	1278:1284	arg1	1129 U ml-1					1296:1306	1129 U ml-1	1296:1306	1129 U ml-1	1296:1306	The highest xylanase (1129 U ml-1) was observed in T. viride + P. chrysosporium co-culture.
32206967	9	56	theme	highest	1278:1284	arg1	xylanase					1286:1293	The highest xylanase	1274:1293	The highest xylanase (1129 U ml-1)	1274:1307	The highest xylanase (1129 U ml-1) was observed in T. viride + P. chrysosporium co-culture.
32206967	1	57	theme	high	246:249	arg1	amount					251:256	high amount	246:256	high amount of reducing sugars	246:275	We evaluated various agricultural lignocellulosic biomass and variety of fungi to produce cellulolytic enzymes cocktail to yield high amount of reducing sugars.
32206967	1	57	theme	high	246:249	arg1	sugars					270:275	reducing sugars	261:275	reducing sugars	261:275	We evaluated various agricultural lignocellulosic biomass and variety of fungi to produce cellulolytic enzymes cocktail to yield high amount of reducing sugars.
32206967	2	58	theme	Solid-state	278:288	arg1	fermentation					290:301	Solid-state fermentation	278:301	Solid-state fermentation	278:301	Solid-state fermentation was performed using water hyacinth, paddy straw, corn straw, soybean husk/tops, wheat straw, and sugarcane bagasse using fungi like Nocardiopsis sp.
32206967	1	59	theme	lignocellulosic	151:165	arg1	biomass					167:173	various agricultural lignocellulosic biomass	130:173	various agricultural lignocellulosic biomass	130:173	We evaluated various agricultural lignocellulosic biomass and variety of fungi to produce cellulolytic enzymes cocktail to yield high amount of reducing sugars.
32206967	2	60	theme	corn	352:355	arg1	straw					357:361	corn straw	352:361	corn straw	352:361	Solid-state fermentation was performed using water hyacinth, paddy straw, corn straw, soybean husk/tops, wheat straw, and sugarcane bagasse using fungi like Nocardiopsis sp.
32206967	1	61	dep	enzymes	220:226	arg1	cocktail					228:235	cocktail	228:235	cocktail	228:235	We evaluated various agricultural lignocellulosic biomass and variety of fungi to produce cellulolytic enzymes cocktail to yield high amount of reducing sugars.
32206967	2	62	theme	wheat	383:387	arg1	straw					389:393	wheat straw	383:393	wheat straw	383:393	Solid-state fermentation was performed using water hyacinth, paddy straw, corn straw, soybean husk/tops, wheat straw, and sugarcane bagasse using fungi like Nocardiopsis sp.
32206967	10	63	theme	good	1443:1446	arg1	quality					1448:1454	good quality	1443:1454	good quality enzyme cocktail to obtain maximum reducing sugars	1443:1504	This study revealed the dependency on substrate and microorganism to produce good quality enzyme cocktail to obtain maximum reducing sugars.
32206967	10	64	theme	enzyme	1456:1461	arg1	cocktail					1463:1470	enzyme cocktail	1456:1470	enzyme cocktail	1456:1470	This study revealed the dependency on substrate and microorganism to produce good quality enzyme cocktail to obtain maximum reducing sugars.
32206967	0	65	theme	Synthesized	15:25	arg1	Enzymes					40:46	Synthesized Cellulolytic Enzymes	15:46	Synthesized Cellulolytic Enzymes	15:46	Composition of Synthesized Cellulolytic Enzymes Varied with the Usage of Agricultural Substrates and Microorganisms.
32206967	3	66	theme	Phanerochaete	521:533	arg1	chrysosporium					535:547	Phanerochaete chrysosporium	521:547	Phanerochaete chrysosporium	521:547	KNU, Trichoderma reesei, Trichoderma viride, Aspergillus flavus, and Phanerochaete chrysosporium alone and in combination to produce cellulolytic enzymes.
32206967	1	67	theme	reducing	261:268	arg1	sugars					270:275	reducing sugars	261:275	reducing sugars	261:275	We evaluated various agricultural lignocellulosic biomass and variety of fungi to produce cellulolytic enzymes cocktail to yield high amount of reducing sugars.
31961287	3	0	theme	gene	314:317	arg1	sequences					319:327	both 16S rRNA gene and rpoB gene sequences	286:327	sequences	319:327	Phylogenetic analyses based on both 16S rRNA gene and rpoB gene sequences showed that MS2379T was most closely related to Paenibacillus polymyxa (DSM 36T), P. jamilae (DSM 13815T), and P. peoriae (DSM 8320T), yet DNA-DNA relatedness through DNA-DNA hybridization revealed only 22.6, 32.0 and 24.7 % relatedness to these three species respectively.
31961287	9	1	dep	%	1424:1424	arg1	3 					1422:1423	3 	1422:1423	3 	1422:1423	The growth range of MS2379T was observed from 10-45 °C with optimal growth temperature at 30 °C. Growth was observed between pH 6-10 and up to 3 % NaCl.
31961287	7	2	theme	gas	1034:1036	arg1	end-products					1038:1049	gas end-products	1034:1049	gas end-products	1034:1049	Fermentation of glucose yields acid and gas end-products.
31961287	11	3	theme	strain	1633:1638	arg1	MS2379T					1640:1646	strain MS2379T	1633:1646	strain MS2379T	1633:1646	Nucleic acid, chemotaxonomic and biochemical features support the distinctiveness of strain MS2379T.
31961287	3	4	theme	DSM	452:454	arg1	peoriae					443:449	P. peoriae	440:449	P. peoriae (DSM 8320T)	440:461	Phylogenetic analyses based on both 16S rRNA gene and rpoB gene sequences showed that MS2379T was most closely related to Paenibacillus polymyxa (DSM 36T), P. jamilae (DSM 13815T), and P. peoriae (DSM 8320T), yet DNA-DNA relatedness through DNA-DNA hybridization revealed only 22.6, 32.0 and 24.7 % relatedness to these three species respectively.
31961287	3	4	theme	DSM	452:454	arg1	8320T					456:460	DSM 8320T	452:460	DSM 8320T	452:460	Phylogenetic analyses based on both 16S rRNA gene and rpoB gene sequences showed that MS2379T was most closely related to Paenibacillus polymyxa (DSM 36T), P. jamilae (DSM 13815T), and P. peoriae (DSM 8320T), yet DNA-DNA relatedness through DNA-DNA hybridization revealed only 22.6, 32.0 and 24.7 % relatedness to these three species respectively.
31961287	8	5	theme	polar	1056:1060	arg1	lipids					1062:1067	The polar lipids	1052:1067	The polar lipids found	1052:1073	The polar lipids found were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and glycolipids, but also included some unidentified lipids, aminolipids, aminoglycolipid, and phosphatidylmethylethanolamine.
31961287	8	5	theme	polar	1056:1060	arg1	diphosphatidylglycerol					1080:1101	diphosphatidylglycerol	1080:1101	diphosphatidylglycerol	1080:1101	The polar lipids found were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and glycolipids, but also included some unidentified lipids, aminolipids, aminoglycolipid, and phosphatidylmethylethanolamine.
31961287	13	6	theme	=DSM	1816:1819	arg1	MS2379T					1807:1813	the type strain MS2379T	1791:1813	the type strain MS2379T (=DSM 107750T=ATCC TSD-165T)	1791:1842	nov. is proposed with the type strain MS2379T (=DSM 107750T=ATCC TSD-165T).
31961287	13	6	theme	=DSM	1816:1819	arg1	TSD-165T					1834:1841	=DSM 107750T=ATCC TSD-165T	1816:1841	=DSM 107750T=ATCC TSD-165T	1816:1841	nov. is proposed with the type strain MS2379T (=DSM 107750T=ATCC TSD-165T).
31961287	2	7	attach	isolated	112:119	arg2	MS2379T					100:106	Strain MS2379T	93:106	Strain MS2379T	93:106	Strain MS2379T was isolated from a pasteurized solution sample from a predominantly anaerobic fermentation system processing bovine manure in Pilot Point, Texas.
31961287	2	7	attach	isolated	112:119	arg1	sample					149:154	a pasteurized solution sample	126:154	a pasteurized solution sample from a predominantly anaerobic fermentation system processing bovine manure	126:230	Strain MS2379T was isolated from a pasteurized solution sample from a predominantly anaerobic fermentation system processing bovine manure in Pilot Point, Texas.
31961287	10	8	theme	Voges-Proskauer	1522:1536	arg1	reaction					1538:1545	the Voges-Proskauer reaction	1518:1545	the Voges-Proskauer reaction	1518:1545	Unlike the most closely related Paenibacillus species, strain MS2379T was negative in the Voges-Proskauer reaction.
31961287	4	9	with	variable	653:660	arg1	endospores					704:713	sub-terminal, ellipsoidal, deforming endospores	667:713	sub-terminal, ellipsoidal, deforming endospores	667:713	Rod-shaped cells of strain MS2379T are Gram-stain variable with sub-terminal, ellipsoidal, deforming endospores.
31961287	1	10	theme	fermentation	47:58	arg1	manure					85:90	a fermentation system processing bovine manure	45:90	a fermentation system processing bovine manure	45:90	nov. isolated from a fermentation system processing bovine manure.
31961287	11	11	theme	chemotaxonomic	1562:1575	arg1	features					1593:1600	Nucleic acid, chemotaxonomic and biochemical features	1548:1600	Nucleic acid, chemotaxonomic and biochemical features	1548:1600	Nucleic acid, chemotaxonomic and biochemical features support the distinctiveness of strain MS2379T.
31961287	11	12	theme	MS2379T	1640:1646	arg1	distinctiveness					1614:1628	the distinctiveness	1610:1628	the distinctiveness of strain MS2379T	1610:1646	Nucleic acid, chemotaxonomic and biochemical features support the distinctiveness of strain MS2379T.
31961287	5	13	theme	strain	882:887	arg1	MS2379T					889:895	strain MS2379T	882:895	strain MS2379T	882:895	The peptidoglycan contains meso-diaminopimelic acid (mDAP) and the predominant fatty acids are anteiso-C15 : 0 (61.9 %) and anteiso-C17 : 0 (11.6 %), confirming that strain MS2379T has diagnostic features of other Paenibacillus species.
31961287	12	14	theme	name	1738:1741	arg1	sp					1765:1766	the name Paenibacillus ottowii sp	1734:1766	the name Paenibacillus ottowii sp	1734:1766	Thus, strain MS2379T represents a novel species of the genus Paenibacillus for which the name Paenibacillus ottowii sp.
31961287	1	15	theme	system	60:65	arg1	manure					85:90	a fermentation system processing bovine manure	45:90	a fermentation system processing bovine manure	45:90	nov. isolated from a fermentation system processing bovine manure.
31961287	5	16	contain	has	897:899	arg1	MS2379T					889:895	strain MS2379T	882:895	strain MS2379T	882:895	The peptidoglycan contains meso-diaminopimelic acid (mDAP) and the predominant fatty acids are anteiso-C15 : 0 (61.9 %) and anteiso-C17 : 0 (11.6 %), confirming that strain MS2379T has diagnostic features of other Paenibacillus species.
31961287	5	16	contain	has	897:899	arg2	features					912:919	diagnostic features	901:919	diagnostic features of other Paenibacillus species	901:950	The peptidoglycan contains meso-diaminopimelic acid (mDAP) and the predominant fatty acids are anteiso-C15 : 0 (61.9 %) and anteiso-C17 : 0 (11.6 %), confirming that strain MS2379T has diagnostic features of other Paenibacillus species.
31961287	4	17	theme	sub-terminal	667:678	arg1	endospores					704:713	sub-terminal, ellipsoidal, deforming endospores	667:713	sub-terminal, ellipsoidal, deforming endospores	667:713	Rod-shaped cells of strain MS2379T are Gram-stain variable with sub-terminal, ellipsoidal, deforming endospores.
31961287	2	18	from	manure	225:230	arg1	sample					149:154	a pasteurized solution sample	126:154	a pasteurized solution sample from a predominantly anaerobic fermentation system processing bovine manure	126:230	Strain MS2379T was isolated from a pasteurized solution sample from a predominantly anaerobic fermentation system processing bovine manure in Pilot Point, Texas.
31961287	3	19	theme	Phylogenetic	255:266	arg1	analyses					268:275	Phylogenetic analyses	255:275	Phylogenetic analyses based on both 16S rRNA gene and rpoB gene sequences	255:327	Phylogenetic analyses based on both 16S rRNA gene and rpoB gene sequences showed that MS2379T was most closely related to Paenibacillus polymyxa (DSM 36T), P. jamilae (DSM 13815T), and P. peoriae (DSM 8320T), yet DNA-DNA relatedness through DNA-DNA hybridization revealed only 22.6, 32.0 and 24.7 % relatedness to these three species respectively.
31961287	1	20	theme	processing	67:76	arg1	manure					85:90	a fermentation system processing bovine manure	45:90	a fermentation system processing bovine manure	45:90	nov. isolated from a fermentation system processing bovine manure.
31961287	8	21	theme	unidentified	1192:1203	arg1	aminoglycolipid					1226:1240	aminoglycolipid	1226:1240	aminoglycolipid	1226:1240	The polar lipids found were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and glycolipids, but also included some unidentified lipids, aminolipids, aminoglycolipid, and phosphatidylmethylethanolamine.
31961287	8	21	theme	unidentified	1192:1203	arg1	phosphatidylmethylethanolamine					1247:1276	phosphatidylmethylethanolamine	1247:1276	phosphatidylmethylethanolamine	1247:1276	The polar lipids found were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and glycolipids, but also included some unidentified lipids, aminolipids, aminoglycolipid, and phosphatidylmethylethanolamine.
31961287	8	21	theme	unidentified	1192:1203	arg1	aminolipids					1213:1223	aminolipids	1213:1223	aminolipids	1213:1223	The polar lipids found were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and glycolipids, but also included some unidentified lipids, aminolipids, aminoglycolipid, and phosphatidylmethylethanolamine.
31961287	8	21	theme	unidentified	1192:1203	arg1	lipids					1205:1210	some unidentified lipids	1187:1210	some unidentified lipids	1187:1210	The polar lipids found were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and glycolipids, but also included some unidentified lipids, aminolipids, aminoglycolipid, and phosphatidylmethylethanolamine.
31961287	4	22	theme	Rod-shaped	603:612	arg1	cells					614:618	Rod-shaped cells	603:618	Rod-shaped cells of strain MS2379T	603:636	Rod-shaped cells of strain MS2379T are Gram-stain variable with sub-terminal, ellipsoidal, deforming endospores.
31961287	2	23	theme	solution	140:147	arg1	sample					149:154	a pasteurized solution sample	126:154	a pasteurized solution sample from a predominantly anaerobic fermentation system processing bovine manure	126:230	Strain MS2379T was isolated from a pasteurized solution sample from a predominantly anaerobic fermentation system processing bovine manure in Pilot Point, Texas.
31961287	1	24	theme	bovine	78:83	arg1	manure					85:90	a fermentation system processing bovine manure	45:90	a fermentation system processing bovine manure	45:90	nov. isolated from a fermentation system processing bovine manure.
31961287	13	25	theme	107750T=ATCC	1821:1832	arg1	MS2379T					1807:1813	the type strain MS2379T	1791:1813	the type strain MS2379T (=DSM 107750T=ATCC TSD-165T)	1791:1842	nov. is proposed with the type strain MS2379T (=DSM 107750T=ATCC TSD-165T).
31961287	13	25	theme	107750T=ATCC	1821:1832	arg1	TSD-165T					1834:1841	=DSM 107750T=ATCC TSD-165T	1816:1841	=DSM 107750T=ATCC TSD-165T	1816:1841	nov. is proposed with the type strain MS2379T (=DSM 107750T=ATCC TSD-165T).
31961287	0	26	theme	ottowii	14:20	arg1	sp					22:23	Paenibacillus ottowii sp	0:23	Paenibacillus ottowii sp.	0:24	Paenibacillus ottowii sp.
31961287	2	27	theme	pasteurized	128:138	arg1	sample					149:154	a pasteurized solution sample	126:154	a pasteurized solution sample from a predominantly anaerobic fermentation system processing bovine manure	126:230	Strain MS2379T was isolated from a pasteurized solution sample from a predominantly anaerobic fermentation system processing bovine manure in Pilot Point, Texas.
31961287	11	28	theme	biochemical	1581:1591	arg1	features					1593:1600	Nucleic acid, chemotaxonomic and biochemical features	1548:1600	Nucleic acid, chemotaxonomic and biochemical features	1548:1600	Nucleic acid, chemotaxonomic and biochemical features support the distinctiveness of strain MS2379T.
31961287	10	29	theme	Paenibacillus	1464:1476	arg1	species					1478:1484	the most closely related Paenibacillus species	1439:1484	the most closely related Paenibacillus species	1439:1484	Unlike the most closely related Paenibacillus species, strain MS2379T was negative in the Voges-Proskauer reaction.
31961287	6	30	theme	45.9 mol	983:990	arg1	%					991:991	45.9 mol%	983:991	45.9 mol%	983:991	The G+C content of MS2379T is 45.9 mol%.
31961287	6	30	theme	45.9 mol	983:990	arg1	content					961:967	The G+C content	953:967	The G+C content of MS2379T	953:978	The G+C content of MS2379T is 45.9 mol%.
31961287	0	31	theme	Paenibacillus	0:12	arg1	sp					22:23	Paenibacillus ottowii sp	0:23	Paenibacillus ottowii sp.	0:24	Paenibacillus ottowii sp.
31961287	12	32	theme	Paenibacillus	1710:1722	arg1	genus					1704:1708	the genus Paenibacillus	1700:1722	the genus Paenibacillus	1700:1722	Thus, strain MS2379T represents a novel species of the genus Paenibacillus for which the name Paenibacillus ottowii sp.
31961287	4	33	dep	sub-terminal	667:678	arg1	deforming					694:702	deforming	694:702	deforming	694:702	Rod-shaped cells of strain MS2379T are Gram-stain variable with sub-terminal, ellipsoidal, deforming endospores.
31961287	4	33	dep	sub-terminal	667:678	arg1	ellipsoidal					681:691	ellipsoidal	681:691	ellipsoidal	681:691	Rod-shaped cells of strain MS2379T are Gram-stain variable with sub-terminal, ellipsoidal, deforming endospores.
31961287	9	34	theme	MS2379T	1299:1305	arg1	range					1290:1294	The growth range	1279:1294	The growth range of MS2379T	1279:1305	The growth range of MS2379T was observed from 10-45 °C with optimal growth temperature at 30 °C. Growth was observed between pH 6-10 and up to 3 % NaCl.
31961287	12	35	theme	genus	1704:1708	arg1	species					1689:1695	a novel species	1681:1695	a novel species of the genus Paenibacillus for which the name Paenibacillus ottowii sp	1681:1766	Thus, strain MS2379T represents a novel species of the genus Paenibacillus for which the name Paenibacillus ottowii sp.
31961287	3	36	theme	DNA-DNA	496:502	arg1	hybridization					504:516	DNA-DNA hybridization	496:516	DNA-DNA hybridization	496:516	Phylogenetic analyses based on both 16S rRNA gene and rpoB gene sequences showed that MS2379T was most closely related to Paenibacillus polymyxa (DSM 36T), P. jamilae (DSM 13815T), and P. peoriae (DSM 8320T), yet DNA-DNA relatedness through DNA-DNA hybridization revealed only 22.6, 32.0 and 24.7 % relatedness to these three species respectively.
31961287	13	37	theme	type	1795:1798	arg1	MS2379T					1807:1813	the type strain MS2379T	1791:1813	the type strain MS2379T (=DSM 107750T=ATCC TSD-165T)	1791:1842	nov. is proposed with the type strain MS2379T (=DSM 107750T=ATCC TSD-165T).
31961287	13	37	theme	type	1795:1798	arg1	TSD-165T					1834:1841	=DSM 107750T=ATCC TSD-165T	1816:1841	=DSM 107750T=ATCC TSD-165T	1816:1841	nov. is proposed with the type strain MS2379T (=DSM 107750T=ATCC TSD-165T).
31961287	5	38	theme	fatty	795:799	arg1	anteiso-C15 					811:822	anteiso-C15 	811:822	anteiso-C15 	811:822	The peptidoglycan contains meso-diaminopimelic acid (mDAP) and the predominant fatty acids are anteiso-C15 : 0 (61.9 %) and anteiso-C17 : 0 (11.6 %), confirming that strain MS2379T has diagnostic features of other Paenibacillus species.
31961287	5	38	theme	fatty	795:799	arg1	acids					801:805	the predominant fatty acids	779:805	the predominant fatty acids	779:805	The peptidoglycan contains meso-diaminopimelic acid (mDAP) and the predominant fatty acids are anteiso-C15 : 0 (61.9 %) and anteiso-C17 : 0 (11.6 %), confirming that strain MS2379T has diagnostic features of other Paenibacillus species.
31961287	5	39	theme	predominant	783:793	arg1	anteiso-C15 					811:822	anteiso-C15 	811:822	anteiso-C15 	811:822	The peptidoglycan contains meso-diaminopimelic acid (mDAP) and the predominant fatty acids are anteiso-C15 : 0 (61.9 %) and anteiso-C17 : 0 (11.6 %), confirming that strain MS2379T has diagnostic features of other Paenibacillus species.
31961287	5	39	theme	predominant	783:793	arg1	acids					801:805	the predominant fatty acids	779:805	the predominant fatty acids	779:805	The peptidoglycan contains meso-diaminopimelic acid (mDAP) and the predominant fatty acids are anteiso-C15 : 0 (61.9 %) and anteiso-C17 : 0 (11.6 %), confirming that strain MS2379T has diagnostic features of other Paenibacillus species.
31961287	3	40	theme	16S	291:293	arg1	gene					300:303	both 16S rRNA gene and rpoB gene sequences	286:327	gene	300:303	Phylogenetic analyses based on both 16S rRNA gene and rpoB gene sequences showed that MS2379T was most closely related to Paenibacillus polymyxa (DSM 36T), P. jamilae (DSM 13815T), and P. peoriae (DSM 8320T), yet DNA-DNA relatedness through DNA-DNA hybridization revealed only 22.6, 32.0 and 24.7 % relatedness to these three species respectively.
31961287	2	41	theme	fermentation	187:198	arg1	manure					225:230	a predominantly anaerobic fermentation system processing bovine manure	161:230	a predominantly anaerobic fermentation system processing bovine manure	161:230	Strain MS2379T was isolated from a pasteurized solution sample from a predominantly anaerobic fermentation system processing bovine manure in Pilot Point, Texas.
31961287	13	42	theme	strain	1800:1805	arg1	MS2379T					1807:1813	the type strain MS2379T	1791:1813	the type strain MS2379T (=DSM 107750T=ATCC TSD-165T)	1791:1842	nov. is proposed with the type strain MS2379T (=DSM 107750T=ATCC TSD-165T).
31961287	13	42	theme	strain	1800:1805	arg1	TSD-165T					1834:1841	=DSM 107750T=ATCC TSD-165T	1816:1841	=DSM 107750T=ATCC TSD-165T	1816:1841	nov. is proposed with the type strain MS2379T (=DSM 107750T=ATCC TSD-165T).
31961287	3	43	dep	%	552:552	arg1	32.0					538:541	32.0	538:541	32.0	538:541	Phylogenetic analyses based on both 16S rRNA gene and rpoB gene sequences showed that MS2379T was most closely related to Paenibacillus polymyxa (DSM 36T), P. jamilae (DSM 13815T), and P. peoriae (DSM 8320T), yet DNA-DNA relatedness through DNA-DNA hybridization revealed only 22.6, 32.0 and 24.7 % relatedness to these three species respectively.
31961287	3	43	dep	%	552:552	arg1	24.7					547:550	24.7	547:550	24.7	547:550	Phylogenetic analyses based on both 16S rRNA gene and rpoB gene sequences showed that MS2379T was most closely related to Paenibacillus polymyxa (DSM 36T), P. jamilae (DSM 13815T), and P. peoriae (DSM 8320T), yet DNA-DNA relatedness through DNA-DNA hybridization revealed only 22.6, 32.0 and 24.7 % relatedness to these three species respectively.
31961287	5	44	theme	meso-diaminopimelic	743:761	arg1	mDAP					769:772	mDAP	769:772	mDAP	769:772	The peptidoglycan contains meso-diaminopimelic acid (mDAP) and the predominant fatty acids are anteiso-C15 : 0 (61.9 %) and anteiso-C17 : 0 (11.6 %), confirming that strain MS2379T has diagnostic features of other Paenibacillus species.
31961287	5	44	theme	meso-diaminopimelic	743:761	arg1	acid					763:766	meso-diaminopimelic acid	743:766	meso-diaminopimelic acid (mDAP)	743:773	The peptidoglycan contains meso-diaminopimelic acid (mDAP) and the predominant fatty acids are anteiso-C15 : 0 (61.9 %) and anteiso-C17 : 0 (11.6 %), confirming that strain MS2379T has diagnostic features of other Paenibacillus species.
31961287	9	45	dep	NaCl	1426:1429	arg1	%					1424:1424	%	1424:1424	%	1424:1424	The growth range of MS2379T was observed from 10-45 °C with optimal growth temperature at 30 °C. Growth was observed between pH 6-10 and up to 3 % NaCl.
31961287	2	46	theme	anaerobic	177:185	arg1	manure					225:230	a predominantly anaerobic fermentation system processing bovine manure	161:230	a predominantly anaerobic fermentation system processing bovine manure	161:230	Strain MS2379T was isolated from a pasteurized solution sample from a predominantly anaerobic fermentation system processing bovine manure in Pilot Point, Texas.
31961287	5	47	theme	Paenibacillus	930:942	arg1	species					944:950	other Paenibacillus species	924:950	other Paenibacillus species	924:950	The peptidoglycan contains meso-diaminopimelic acid (mDAP) and the predominant fatty acids are anteiso-C15 : 0 (61.9 %) and anteiso-C17 : 0 (11.6 %), confirming that strain MS2379T has diagnostic features of other Paenibacillus species.
31961287	3	48	theme	DSM	423:425	arg1	jamilae					414:420	jamilae	414:420	jamilae	414:420	Phylogenetic analyses based on both 16S rRNA gene and rpoB gene sequences showed that MS2379T was most closely related to Paenibacillus polymyxa (DSM 36T), P. jamilae (DSM 13815T), and P. peoriae (DSM 8320T), yet DNA-DNA relatedness through DNA-DNA hybridization revealed only 22.6, 32.0 and 24.7 % relatedness to these three species respectively.
31961287	3	48	theme	DSM	423:425	arg1	13815T					427:432	DSM 13815T	423:432	DSM 13815T	423:432	Phylogenetic analyses based on both 16S rRNA gene and rpoB gene sequences showed that MS2379T was most closely related to Paenibacillus polymyxa (DSM 36T), P. jamilae (DSM 13815T), and P. peoriae (DSM 8320T), yet DNA-DNA relatedness through DNA-DNA hybridization revealed only 22.6, 32.0 and 24.7 % relatedness to these three species respectively.
31961287	9	49	dep	3 	1422:1423	arg1	to					1419:1420	to	1419:1420	to	1419:1420	The growth range of MS2379T was observed from 10-45 °C with optimal growth temperature at 30 °C. Growth was observed between pH 6-10 and up to 3 % NaCl.
31961287	5	50	theme	species	944:950	arg1	features					912:919	diagnostic features	901:919	diagnostic features of other Paenibacillus species	901:950	The peptidoglycan contains meso-diaminopimelic acid (mDAP) and the predominant fatty acids are anteiso-C15 : 0 (61.9 %) and anteiso-C17 : 0 (11.6 %), confirming that strain MS2379T has diagnostic features of other Paenibacillus species.
31961287	11	51	theme	Nucleic	1548:1554	arg1	features					1593:1600	Nucleic acid, chemotaxonomic and biochemical features	1548:1600	Nucleic acid, chemotaxonomic and biochemical features	1548:1600	Nucleic acid, chemotaxonomic and biochemical features support the distinctiveness of strain MS2379T.
31961287	6	52	theme	G+C	957:959	arg1	%					991:991	45.9 mol%	983:991	45.9 mol%	983:991	The G+C content of MS2379T is 45.9 mol%.
31961287	6	52	theme	G+C	957:959	arg1	content					961:967	The G+C content	953:967	The G+C content of MS2379T	953:978	The G+C content of MS2379T is 45.9 mol%.
31961287	10	53	theme	related	1456:1462	arg1	species					1478:1484	the most closely related Paenibacillus species	1439:1484	the most closely related Paenibacillus species	1439:1484	Unlike the most closely related Paenibacillus species, strain MS2379T was negative in the Voges-Proskauer reaction.
31961287	2	54	theme	Strain	93:98	arg1	MS2379T					100:106	Strain MS2379T	93:106	Strain MS2379T	93:106	Strain MS2379T was isolated from a pasteurized solution sample from a predominantly anaerobic fermentation system processing bovine manure in Pilot Point, Texas.
31961287	9	55	dep	temperature	1354:1364	arg1	observed					1387:1394	observed	1387:1394	was observed between pH 6-10 and up to 3 % NaCl	1383:1429	The growth range of MS2379T was observed from 10-45 °C with optimal growth temperature at 30 °C. Growth was observed between pH 6-10 and up to 3 % NaCl.
31961287	3	56	dep	relatedness	554:564	arg1	%					552:552	%	552:552	%	552:552	Phylogenetic analyses based on both 16S rRNA gene and rpoB gene sequences showed that MS2379T was most closely related to Paenibacillus polymyxa (DSM 36T), P. jamilae (DSM 13815T), and P. peoriae (DSM 8320T), yet DNA-DNA relatedness through DNA-DNA hybridization revealed only 22.6, 32.0 and 24.7 % relatedness to these three species respectively.
31961287	9	57	theme	growth	1283:1288	arg1	range					1290:1294	The growth range	1279:1294	The growth range of MS2379T	1279:1305	The growth range of MS2379T was observed from 10-45 °C with optimal growth temperature at 30 °C. Growth was observed between pH 6-10 and up to 3 % NaCl.
31961287	3	58	theme	DSM	401:403	arg1	polymyxa					391:398	Paenibacillus polymyxa	377:398	Paenibacillus polymyxa (DSM 36T)	377:408	Phylogenetic analyses based on both 16S rRNA gene and rpoB gene sequences showed that MS2379T was most closely related to Paenibacillus polymyxa (DSM 36T), P. jamilae (DSM 13815T), and P. peoriae (DSM 8320T), yet DNA-DNA relatedness through DNA-DNA hybridization revealed only 22.6, 32.0 and 24.7 % relatedness to these three species respectively.
31961287	3	58	theme	DSM	401:403	arg1	36T					405:407	DSM 36T	401:407	DSM 36T	401:407	Phylogenetic analyses based on both 16S rRNA gene and rpoB gene sequences showed that MS2379T was most closely related to Paenibacillus polymyxa (DSM 36T), P. jamilae (DSM 13815T), and P. peoriae (DSM 8320T), yet DNA-DNA relatedness through DNA-DNA hybridization revealed only 22.6, 32.0 and 24.7 % relatedness to these three species respectively.
31961287	3	59	theme	rpoB	309:312	arg1	sequences					319:327	both 16S rRNA gene and rpoB gene sequences	286:327	sequences	319:327	Phylogenetic analyses based on both 16S rRNA gene and rpoB gene sequences showed that MS2379T was most closely related to Paenibacillus polymyxa (DSM 36T), P. jamilae (DSM 13815T), and P. peoriae (DSM 8320T), yet DNA-DNA relatedness through DNA-DNA hybridization revealed only 22.6, 32.0 and 24.7 % relatedness to these three species respectively.
31961287	10	60	theme	strain	1487:1492	arg1	MS2379T					1494:1500	strain MS2379T	1487:1500	strain MS2379T	1487:1500	Unlike the most closely related Paenibacillus species, strain MS2379T was negative in the Voges-Proskauer reaction.
31961287	10	61	from	negative	1506:1513	arg1	reaction					1538:1545	the Voges-Proskauer reaction	1518:1545	the Voges-Proskauer reaction	1518:1545	Unlike the most closely related Paenibacillus species, strain MS2379T was negative in the Voges-Proskauer reaction.
31961287	10	62	from	reaction	1538:1545	arg1	negative					1506:1513	negative	1506:1513	negative	1506:1513	Unlike the most closely related Paenibacillus species, strain MS2379T was negative in the Voges-Proskauer reaction.
31961287	9	63	theme	optimal	1339:1345	arg1	temperature					1354:1364	optimal growth temperature	1339:1364	optimal growth temperature at 30 °C. Growth was observed between pH 6-10 and up to 3 % NaCl	1339:1429	The growth range of MS2379T was observed from 10-45 °C with optimal growth temperature at 30 °C. Growth was observed between pH 6-10 and up to 3 % NaCl.
31961287	3	64	theme	rRNA	295:298	arg1	gene					300:303	both 16S rRNA gene and rpoB gene sequences	286:327	gene	300:303	Phylogenetic analyses based on both 16S rRNA gene and rpoB gene sequences showed that MS2379T was most closely related to Paenibacillus polymyxa (DSM 36T), P. jamilae (DSM 13815T), and P. peoriae (DSM 8320T), yet DNA-DNA relatedness through DNA-DNA hybridization revealed only 22.6, 32.0 and 24.7 % relatedness to these three species respectively.
31961287	2	65	theme	bovine	218:223	arg1	manure					225:230	a predominantly anaerobic fermentation system processing bovine manure	161:230	a predominantly anaerobic fermentation system processing bovine manure	161:230	Strain MS2379T was isolated from a pasteurized solution sample from a predominantly anaerobic fermentation system processing bovine manure in Pilot Point, Texas.
31961287	5	66	theme	diagnostic	901:910	arg1	features					912:919	diagnostic features	901:919	diagnostic features of other Paenibacillus species	901:950	The peptidoglycan contains meso-diaminopimelic acid (mDAP) and the predominant fatty acids are anteiso-C15 : 0 (61.9 %) and anteiso-C17 : 0 (11.6 %), confirming that strain MS2379T has diagnostic features of other Paenibacillus species.
31961287	7	67	theme	glucose	1010:1016	arg1	Fermentation					994:1005	Fermentation	994:1005	Fermentation of glucose	994:1016	Fermentation of glucose yields acid and gas end-products.
31961287	9	68	theme	growth	1347:1352	arg1	temperature					1354:1364	optimal growth temperature	1339:1364	optimal growth temperature at 30 °C. Growth was observed between pH 6-10 and up to 3 % NaCl	1339:1429	The growth range of MS2379T was observed from 10-45 °C with optimal growth temperature at 30 °C. Growth was observed between pH 6-10 and up to 3 % NaCl.
31961287	3	69	theme	DNA-DNA	468:474	arg1	relatedness					476:486	DNA-DNA relatedness	468:486	DNA-DNA relatedness through DNA-DNA hybridization	468:516	Phylogenetic analyses based on both 16S rRNA gene and rpoB gene sequences showed that MS2379T was most closely related to Paenibacillus polymyxa (DSM 36T), P. jamilae (DSM 13815T), and P. peoriae (DSM 8320T), yet DNA-DNA relatedness through DNA-DNA hybridization revealed only 22.6, 32.0 and 24.7 % relatedness to these three species respectively.
31961287	2	70	theme	processing	207:216	arg1	manure					225:230	a predominantly anaerobic fermentation system processing bovine manure	161:230	a predominantly anaerobic fermentation system processing bovine manure	161:230	Strain MS2379T was isolated from a pasteurized solution sample from a predominantly anaerobic fermentation system processing bovine manure in Pilot Point, Texas.
31961287	5	71	dep	anteiso-C15 	811:822	arg1	 0					824:825	 0	824:825	 0	824:825	The peptidoglycan contains meso-diaminopimelic acid (mDAP) and the predominant fatty acids are anteiso-C15 : 0 (61.9 %) and anteiso-C17 : 0 (11.6 %), confirming that strain MS2379T has diagnostic features of other Paenibacillus species.
31961287	5	71	dep	anteiso-C15 	811:822	arg1	%					833:833	61.9 %	828:833	61.9 %	828:833	The peptidoglycan contains meso-diaminopimelic acid (mDAP) and the predominant fatty acids are anteiso-C15 : 0 (61.9 %) and anteiso-C17 : 0 (11.6 %), confirming that strain MS2379T has diagnostic features of other Paenibacillus species.
31961287	5	71	dep	anteiso-C15 	811:822	arg1	anteiso-C17 					840:851	anteiso-C17 	840:851	anteiso-C17 	840:851	The peptidoglycan contains meso-diaminopimelic acid (mDAP) and the predominant fatty acids are anteiso-C15 : 0 (61.9 %) and anteiso-C17 : 0 (11.6 %), confirming that strain MS2379T has diagnostic features of other Paenibacillus species.
31961287	5	71	dep	anteiso-C15 	811:822	arg1	%					862:862	11.6 %	857:862	11.6 %	857:862	The peptidoglycan contains meso-diaminopimelic acid (mDAP) and the predominant fatty acids are anteiso-C15 : 0 (61.9 %) and anteiso-C17 : 0 (11.6 %), confirming that strain MS2379T has diagnostic features of other Paenibacillus species.
31961287	5	71	dep	anteiso-C15 	811:822	arg1	 0					853:854	 0	853:854	 0	853:854	The peptidoglycan contains meso-diaminopimelic acid (mDAP) and the predominant fatty acids are anteiso-C15 : 0 (61.9 %) and anteiso-C17 : 0 (11.6 %), confirming that strain MS2379T has diagnostic features of other Paenibacillus species.
31961287	12	72	theme	ottowii	1757:1763	arg1	sp					1765:1766	the name Paenibacillus ottowii sp	1734:1766	the name Paenibacillus ottowii sp	1734:1766	Thus, strain MS2379T represents a novel species of the genus Paenibacillus for which the name Paenibacillus ottowii sp.
31961287	4	73	theme	strain	623:628	arg1	MS2379T					630:636	strain MS2379T	623:636	strain MS2379T	623:636	Rod-shaped cells of strain MS2379T are Gram-stain variable with sub-terminal, ellipsoidal, deforming endospores.
31961287	11	74	theme	acid	1556:1559	arg1	features					1593:1600	Nucleic acid, chemotaxonomic and biochemical features	1548:1600	Nucleic acid, chemotaxonomic and biochemical features	1548:1600	Nucleic acid, chemotaxonomic and biochemical features support the distinctiveness of strain MS2379T.
31961287	12	75	theme	Paenibacillus	1743:1755	arg1	sp					1765:1766	the name Paenibacillus ottowii sp	1734:1766	the name Paenibacillus ottowii sp	1734:1766	Thus, strain MS2379T represents a novel species of the genus Paenibacillus for which the name Paenibacillus ottowii sp.
31961287	2	76	theme	system	200:205	arg1	manure					225:230	a predominantly anaerobic fermentation system processing bovine manure	161:230	a predominantly anaerobic fermentation system processing bovine manure	161:230	Strain MS2379T was isolated from a pasteurized solution sample from a predominantly anaerobic fermentation system processing bovine manure in Pilot Point, Texas.
31961287	6	77	theme	MS2379T	972:978	arg1	%					991:991	45.9 mol%	983:991	45.9 mol%	983:991	The G+C content of MS2379T is 45.9 mol%.
31961287	6	77	theme	MS2379T	972:978	arg1	content					961:967	The G+C content	953:967	The G+C content of MS2379T	953:978	The G+C content of MS2379T is 45.9 mol%.
31961287	4	78	theme	MS2379T	630:636	arg1	cells					614:618	Rod-shaped cells	603:618	Rod-shaped cells of strain MS2379T	603:636	Rod-shaped cells of strain MS2379T are Gram-stain variable with sub-terminal, ellipsoidal, deforming endospores.
31961287	12	79	theme	novel	1683:1687	arg1	species					1689:1695	a novel species	1681:1695	a novel species of the genus Paenibacillus for which the name Paenibacillus ottowii sp	1681:1766	Thus, strain MS2379T represents a novel species of the genus Paenibacillus for which the name Paenibacillus ottowii sp.
31961287	5	80	contain	contains	734:741	arg2	mDAP					769:772	mDAP	769:772	mDAP	769:772	The peptidoglycan contains meso-diaminopimelic acid (mDAP) and the predominant fatty acids are anteiso-C15 : 0 (61.9 %) and anteiso-C17 : 0 (11.6 %), confirming that strain MS2379T has diagnostic features of other Paenibacillus species.
31961287	5	80	contain	contains	734:741	arg1	peptidoglycan					720:732	The peptidoglycan	716:732	The peptidoglycan	716:732	The peptidoglycan contains meso-diaminopimelic acid (mDAP) and the predominant fatty acids are anteiso-C15 : 0 (61.9 %) and anteiso-C17 : 0 (11.6 %), confirming that strain MS2379T has diagnostic features of other Paenibacillus species.
31961287	5	80	contain	contains	734:741	arg2	acid					763:766	meso-diaminopimelic acid	743:766	meso-diaminopimelic acid (mDAP)	743:773	The peptidoglycan contains meso-diaminopimelic acid (mDAP) and the predominant fatty acids are anteiso-C15 : 0 (61.9 %) and anteiso-C17 : 0 (11.6 %), confirming that strain MS2379T has diagnostic features of other Paenibacillus species.
31961287	5	81	theme	other	924:928	arg1	species					944:950	other Paenibacillus species	924:950	other Paenibacillus species	924:950	The peptidoglycan contains meso-diaminopimelic acid (mDAP) and the predominant fatty acids are anteiso-C15 : 0 (61.9 %) and anteiso-C17 : 0 (11.6 %), confirming that strain MS2379T has diagnostic features of other Paenibacillus species.
31961287	3	82	theme	Paenibacillus	377:389	arg1	polymyxa					391:398	Paenibacillus polymyxa	377:398	Paenibacillus polymyxa (DSM 36T)	377:408	Phylogenetic analyses based on both 16S rRNA gene and rpoB gene sequences showed that MS2379T was most closely related to Paenibacillus polymyxa (DSM 36T), P. jamilae (DSM 13815T), and P. peoriae (DSM 8320T), yet DNA-DNA relatedness through DNA-DNA hybridization revealed only 22.6, 32.0 and 24.7 % relatedness to these three species respectively.
31961287	3	82	theme	Paenibacillus	377:389	arg1	36T					405:407	DSM 36T	401:407	DSM 36T	401:407	Phylogenetic analyses based on both 16S rRNA gene and rpoB gene sequences showed that MS2379T was most closely related to Paenibacillus polymyxa (DSM 36T), P. jamilae (DSM 13815T), and P. peoriae (DSM 8320T), yet DNA-DNA relatedness through DNA-DNA hybridization revealed only 22.6, 32.0 and 24.7 % relatedness to these three species respectively.
31961287	12	83	theme	strain	1655:1660	arg1	MS2379T					1662:1668	strain MS2379T	1655:1668	strain MS2379T	1655:1668	Thus, strain MS2379T represents a novel species of the genus Paenibacillus for which the name Paenibacillus ottowii sp.
31961287	9	84	theme	30 °C.	1369:1374	arg1	Growth					1376:1381	30 °C. Growth	1369:1381	30 °C. Growth	1369:1381	The growth range of MS2379T was observed from 10-45 °C with optimal growth temperature at 30 °C. Growth was observed between pH 6-10 and up to 3 % NaCl.
34294283	7	0	theme	full	1209:1212	arg1	reconstruction					1219:1232	autologous full face reconstruction	1198:1232	autologous full face reconstruction	1198:1232	The hydrogels prepared using these three polysaccharides have great potential in the construction of personalized, complex, and vascularized tissue-engineered anatomical faces and provide a new strategy for autologous full face reconstruction.
34294283	5	1	theme	present	637:643	arg1	study					645:649	The present study	633:649	The present study	633:649	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	5	2	dep	collapse	866:873	arg1	parts					891:895	characteristic parts	876:895	characteristic parts	876:895	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	5	2	dep	collapse	866:873	arg1	lips					906:909	lips	906:909	lips	906:909	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	5	3	theme	characteristic	876:889	arg1	parts					891:895	characteristic parts	876:895	characteristic parts	876:895	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	4	4	from	limited	581:587	arg1	size					592:595	size	592:595	size	592:595	However, simple hydrogels are prone to collapse during printing, are limited in size, and have poor shape and structure.
34294283	6	5	theme	vessel-like	968:978	arg1	structure					980:988	a blood vessel-like structure	960:988	a blood vessel-like structure	960:988	The nutrient network gradually formed a blood vessel-like structure.
34294283	2	6	theme	collateral	396:405	arg1	damage					407:412	collateral damage	396:412	collateral damage	396:412	Autologous tissue reconstruction or allograft face transplantation has been adopted but has problems such as blood supply difficulties, collateral damage, immune rejection, and ethical disputes.
34294283	7	7	theme	great	1053:1057	arg1	potential					1059:1067	great potential	1053:1067	great potential in the construction of personalized, complex, and vascularized tissue-engineered anatomical faces	1053:1165	The hydrogels prepared using these three polysaccharides have great potential in the construction of personalized, complex, and vascularized tissue-engineered anatomical faces and provide a new strategy for autologous full face reconstruction.
34294283	1	8	theme	clinical	241:248	arg1	practice					250:257	clinical practice	241:257	clinical practice	241:257	Large, deep, complex, and severe tissue defects and deformities of the face are the problems encountered in clinical practice.
34294283	2	9	contain	has	348:350	arg2	rejection					422:430	immune rejection	415:430	immune rejection	415:430	Autologous tissue reconstruction or allograft face transplantation has been adopted but has problems such as blood supply difficulties, collateral damage, immune rejection, and ethical disputes.
34294283	2	9	contain	has	348:350	arg2	difficulties					382:393	blood supply difficulties	369:393	blood supply difficulties	369:393	Autologous tissue reconstruction or allograft face transplantation has been adopted but has problems such as blood supply difficulties, collateral damage, immune rejection, and ethical disputes.
34294283	2	9	contain	has	348:350	arg1	reconstruction					278:291	Autologous tissue reconstruction or allograft face transplantation	260:325	reconstruction	278:291	Autologous tissue reconstruction or allograft face transplantation has been adopted but has problems such as blood supply difficulties, collateral damage, immune rejection, and ethical disputes.
34294283	2	9	contain	has	348:350	arg2	problems					352:359	problems	352:359	problems such as blood supply difficulties, collateral damage, immune rejection, and ethical disputes	352:452	Autologous tissue reconstruction or allograft face transplantation has been adopted but has problems such as blood supply difficulties, collateral damage, immune rejection, and ethical disputes.
34294283	2	9	contain	has	348:350	arg2	disputes					445:452	ethical disputes	437:452	ethical disputes	437:452	Autologous tissue reconstruction or allograft face transplantation has been adopted but has problems such as blood supply difficulties, collateral damage, immune rejection, and ethical disputes.
34294283	2	9	contain	has	348:350	arg2	damage					407:412	collateral damage	396:412	collateral damage	396:412	Autologous tissue reconstruction or allograft face transplantation has been adopted but has problems such as blood supply difficulties, collateral damage, immune rejection, and ethical disputes.
34294283	7	10	theme	personalized	1092:1103	arg1	faces					1161:1165	personalized, complex, and vascularized tissue-engineered anatomical faces	1092:1165	personalized, complex, and vascularized tissue-engineered anatomical faces	1092:1165	The hydrogels prepared using these three polysaccharides have great potential in the construction of personalized, complex, and vascularized tissue-engineered anatomical faces and provide a new strategy for autologous full face reconstruction.
34294283	4	11	from	size	592:595	arg1	limited					581:587	limited	581:587	limited	581:587	However, simple hydrogels are prone to collapse during printing, are limited in size, and have poor shape and structure.
34294283	7	12	theme	tissue-engineered	1132:1148	arg1	faces					1161:1165	personalized, complex, and vascularized tissue-engineered anatomical faces	1092:1165	personalized, complex, and vascularized tissue-engineered anatomical faces	1092:1165	The hydrogels prepared using these three polysaccharides have great potential in the construction of personalized, complex, and vascularized tissue-engineered anatomical faces and provide a new strategy for autologous full face reconstruction.
34294283	3	13	theme	tissue	491:496	arg1	regeneration					498:509	personalized tissue regeneration	478:509	personalized tissue regeneration	478:509	3D bioprinting enables personalized tissue regeneration.
34294283	5	14	theme	alginate	735:742	arg1	composites					686:695	three polysaccharide hydrogel composites	656:695	three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA)	656:799	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	5	14	theme	alginate	735:742	arg1	alginate					735:742	sodium alginate	728:742	sodium alginate	728:742	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	5	14	theme	alginate	735:742	arg1	agarose					715:721	agarose	715:721	agarose	715:721	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	5	14	theme	alginate	735:742	arg1	nanocellulose					700:712	nanocellulose	700:712	nanocellulose	700:712	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	2	15	theme	tissue	271:276	arg1	reconstruction					278:291	Autologous tissue reconstruction or allograft face transplantation	260:325	reconstruction	278:291	Autologous tissue reconstruction or allograft face transplantation has been adopted but has problems such as blood supply difficulties, collateral damage, immune rejection, and ethical disputes.
34294283	1	16	theme	severe	159:164	arg1	defects					173:179	tissue defects	166:179	tissue defects	166:179	Large, deep, complex, and severe tissue defects and deformities of the face are the problems encountered in clinical practice.
34294283	1	16	theme	severe	159:164	arg1	problems					217:224	the problems	213:224	the problems encountered in clinical practice	213:257	Large, deep, complex, and severe tissue defects and deformities of the face are the problems encountered in clinical practice.
34294283	1	16	theme	severe	159:164	arg1	deformities					185:195	deformities	185:195	deformities	185:195	Large, deep, complex, and severe tissue defects and deformities of the face are the problems encountered in clinical practice.
34294283	0	17	theme	nutrient	114:121	arg1	networks					123:130	nutrient networks	114:130	nutrient networks	114:130	Agarose composite hydrogel and PVA sacrificial materials for bioprinting large-scale, personalized face-like with nutrient networks.
34294283	5	18	dep	such	898:901	arg1	as					903:904	as	903:904	as	903:904	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	2	19	theme	ethical	437:443	arg1	disputes					445:452	ethical disputes	437:452	ethical disputes	437:452	Autologous tissue reconstruction or allograft face transplantation has been adopted but has problems such as blood supply difficulties, collateral damage, immune rejection, and ethical disputes.
34294283	7	20	from	potential	1059:1067	arg1	construction					1076:1087	the construction	1072:1087	the construction of personalized, complex, and vascularized tissue-engineered anatomical faces	1072:1165	The hydrogels prepared using these three polysaccharides have great potential in the construction of personalized, complex, and vascularized tissue-engineered anatomical faces and provide a new strategy for autologous full face reconstruction.
34294283	1	21	theme	tissue	166:171	arg1	defects					173:179	tissue defects	166:179	tissue defects	166:179	Large, deep, complex, and severe tissue defects and deformities of the face are the problems encountered in clinical practice.
34294283	1	21	theme	tissue	166:171	arg1	problems					217:224	the problems	213:224	the problems encountered in clinical practice	213:257	Large, deep, complex, and severe tissue defects and deformities of the face are the problems encountered in clinical practice.
34294283	1	21	theme	tissue	166:171	arg1	deformities					185:195	deformities	185:195	deformities	185:195	Large, deep, complex, and severe tissue defects and deformities of the face are the problems encountered in clinical practice.
34294283	0	22	theme	composite	8:16	arg1	hydrogel					18:25	Agarose composite hydrogel and PVA sacrificial materials	0:55	hydrogel	18:25	Agarose composite hydrogel and PVA sacrificial materials for bioprinting large-scale, personalized face-like with nutrient networks.
34294283	7	23	theme	face	1214:1217	arg1	reconstruction					1219:1232	autologous full face reconstruction	1198:1232	autologous full face reconstruction	1198:1232	The hydrogels prepared using these three polysaccharides have great potential in the construction of personalized, complex, and vascularized tissue-engineered anatomical faces and provide a new strategy for autologous full face reconstruction.
34294283	5	24	theme	seeded	749:754	arg1	cells					756:760	seeded cells	749:760	seeded cells as bioinks and polyvinyl alcohol (PVA)	749:799	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	0	25	theme	Agarose	0:6	arg1	hydrogel					18:25	Agarose composite hydrogel and PVA sacrificial materials	0:55	hydrogel	18:25	Agarose composite hydrogel and PVA sacrificial materials for bioprinting large-scale, personalized face-like with nutrient networks.
34294283	7	26	theme	anatomical	1150:1159	arg1	faces					1161:1165	personalized, complex, and vascularized tissue-engineered anatomical faces	1092:1165	personalized, complex, and vascularized tissue-engineered anatomical faces	1092:1165	The hydrogels prepared using these three polysaccharides have great potential in the construction of personalized, complex, and vascularized tissue-engineered anatomical faces and provide a new strategy for autologous full face reconstruction.
34294283	5	27	used	used	651:654	arg2	study					645:649	The present study	633:649	The present study	633:649	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	7	28	contain	have	1048:1051	arg2	potential					1059:1067	great potential	1053:1067	great potential in the construction of personalized, complex, and vascularized tissue-engineered anatomical faces	1053:1165	The hydrogels prepared using these three polysaccharides have great potential in the construction of personalized, complex, and vascularized tissue-engineered anatomical faces and provide a new strategy for autologous full face reconstruction.
34294283	7	28	contain	have	1048:1051	arg1	hydrogels					995:1003	The hydrogels	991:1003	The hydrogels prepared using these three polysaccharides	991:1046	The hydrogels prepared using these three polysaccharides have great potential in the construction of personalized, complex, and vascularized tissue-engineered anatomical faces and provide a new strategy for autologous full face reconstruction.
34294283	2	29	theme	supply	375:380	arg1	difficulties					382:393	blood supply difficulties	369:393	blood supply difficulties	369:393	Autologous tissue reconstruction or allograft face transplantation has been adopted but has problems such as blood supply difficulties, collateral damage, immune rejection, and ethical disputes.
34294283	7	30	theme	faces	1161:1165	arg1	construction					1076:1087	the construction	1072:1087	the construction of personalized, complex, and vascularized tissue-engineered anatomical faces	1072:1165	The hydrogels prepared using these three polysaccharides have great potential in the construction of personalized, complex, and vascularized tissue-engineered anatomical faces and provide a new strategy for autologous full face reconstruction.
34294283	5	31	theme	nanocellulose	700:712	arg1	composites					686:695	three polysaccharide hydrogel composites	656:695	three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA)	656:799	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	5	31	theme	nanocellulose	700:712	arg1	alginate					735:742	sodium alginate	728:742	sodium alginate	728:742	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	5	31	theme	nanocellulose	700:712	arg1	agarose					715:721	agarose	715:721	agarose	715:721	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	5	31	theme	nanocellulose	700:712	arg1	nanocellulose					700:712	nanocellulose	700:712	nanocellulose	700:712	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	0	32	with	face-like	99:107	arg1	networks					123:130	nutrient networks	114:130	nutrient networks	114:130	Agarose composite hydrogel and PVA sacrificial materials for bioprinting large-scale, personalized face-like with nutrient networks.
34294283	2	33	theme	face	306:309	arg1	transplantation					311:325	Autologous tissue reconstruction or allograft face transplantation	260:325	transplantation	311:325	Autologous tissue reconstruction or allograft face transplantation has been adopted but has problems such as blood supply difficulties, collateral damage, immune rejection, and ethical disputes.
34294283	5	34	theme	sacrificial	804:814	arg1	material					816:823	sacrificial material	804:823	sacrificial material	804:823	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	6	35	theme	nutrient	926:933	arg1	network					935:941	The nutrient network	922:941	The nutrient network	922:941	The nutrient network gradually formed a blood vessel-like structure.
34294283	2	36	theme	allograft	296:304	arg1	transplantation					311:325	Autologous tissue reconstruction or allograft face transplantation	260:325	transplantation	311:325	Autologous tissue reconstruction or allograft face transplantation has been adopted but has problems such as blood supply difficulties, collateral damage, immune rejection, and ethical disputes.
34294283	2	37	theme	immune	415:420	arg1	rejection					422:430	immune rejection	415:430	immune rejection	415:430	Autologous tissue reconstruction or allograft face transplantation has been adopted but has problems such as blood supply difficulties, collateral damage, immune rejection, and ethical disputes.
34294283	0	38	theme	sacrificial	35:45	arg1	materials					47:55	Agarose composite hydrogel and PVA sacrificial materials	0:55	materials	47:55	Agarose composite hydrogel and PVA sacrificial materials for bioprinting large-scale, personalized face-like with nutrient networks.
34294283	5	39	theme	agarose	715:721	arg1	composites					686:695	three polysaccharide hydrogel composites	656:695	three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA)	656:799	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	5	39	theme	agarose	715:721	arg1	alginate					735:742	sodium alginate	728:742	sodium alginate	728:742	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	5	39	theme	agarose	715:721	arg1	agarose					715:721	agarose	715:721	agarose	715:721	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	5	39	theme	agarose	715:721	arg1	nanocellulose					700:712	nanocellulose	700:712	nanocellulose	700:712	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	0	40	theme	PVA	31:33	arg1	materials					47:55	Agarose composite hydrogel and PVA sacrificial materials	0:55	materials	47:55	Agarose composite hydrogel and PVA sacrificial materials for bioprinting large-scale, personalized face-like with nutrient networks.
34294283	7	41	theme	complex	1106:1112	arg1	faces					1161:1165	personalized, complex, and vascularized tissue-engineered anatomical faces	1092:1165	personalized, complex, and vascularized tissue-engineered anatomical faces	1092:1165	The hydrogels prepared using these three polysaccharides have great potential in the construction of personalized, complex, and vascularized tissue-engineered anatomical faces and provide a new strategy for autologous full face reconstruction.
34294283	5	42	theme	polyvinyl	777:785	arg1	alcohol					787:793	polyvinyl alcohol	777:793	polyvinyl alcohol (PVA)	777:799	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	5	42	theme	polyvinyl	777:785	arg1	PVA					796:798	PVA	796:798	PVA	796:798	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	5	43	theme	sodium	728:733	arg1	alginate					735:742	sodium alginate	728:742	sodium alginate	728:742	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	1	44	theme	face	204:207	arg1	defects					173:179	tissue defects	166:179	tissue defects	166:179	Large, deep, complex, and severe tissue defects and deformities of the face are the problems encountered in clinical practice.
34294283	1	44	theme	face	204:207	arg1	problems					217:224	the problems	213:224	the problems encountered in clinical practice	213:257	Large, deep, complex, and severe tissue defects and deformities of the face are the problems encountered in clinical practice.
34294283	1	44	theme	face	204:207	arg1	deformities					185:195	deformities	185:195	deformities	185:195	Large, deep, complex, and severe tissue defects and deformities of the face are the problems encountered in clinical practice.
34294283	5	45	theme	polysaccharide	662:675	arg1	composites					686:695	three polysaccharide hydrogel composites	656:695	three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA)	656:799	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	5	45	theme	polysaccharide	662:675	arg1	alginate					735:742	sodium alginate	728:742	sodium alginate	728:742	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	5	45	theme	polysaccharide	662:675	arg1	agarose					715:721	agarose	715:721	agarose	715:721	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	5	45	theme	polysaccharide	662:675	arg1	nanocellulose					700:712	nanocellulose	700:712	nanocellulose	700:712	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	4	46	theme	simple	521:526	arg1	hydrogels					528:536	simple hydrogels	521:536	simple hydrogels	521:536	However, simple hydrogels are prone to collapse during printing, are limited in size, and have poor shape and structure.
34294283	5	47	theme	hydrogel	677:684	arg1	composites					686:695	three polysaccharide hydrogel composites	656:695	three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA)	656:799	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	5	47	theme	hydrogel	677:684	arg1	alginate					735:742	sodium alginate	728:742	sodium alginate	728:742	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	5	47	theme	hydrogel	677:684	arg1	agarose					715:721	agarose	715:721	agarose	715:721	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	5	47	theme	hydrogel	677:684	arg1	nanocellulose					700:712	nanocellulose	700:712	nanocellulose	700:712	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	5	48	with	composites	686:695	arg1	cells					756:760	seeded cells	749:760	seeded cells as bioinks and polyvinyl alcohol (PVA)	749:799	The present study used three polysaccharide hydrogel composites of nanocellulose, agarose, and sodium alginate with seeded cells as bioinks and polyvinyl alcohol (PVA) as sacrificial material to construct the structures that did not collapse (characteristic parts, such as lips and nose).
34294283	7	49	theme	autologous	1198:1207	arg1	reconstruction					1219:1232	autologous full face reconstruction	1198:1232	autologous full face reconstruction	1198:1232	The hydrogels prepared using these three polysaccharides have great potential in the construction of personalized, complex, and vascularized tissue-engineered anatomical faces and provide a new strategy for autologous full face reconstruction.
34294283	1	50	theme	Large	133:137	arg1	defects					173:179	tissue defects	166:179	tissue defects	166:179	Large, deep, complex, and severe tissue defects and deformities of the face are the problems encountered in clinical practice.
34294283	1	50	theme	Large	133:137	arg1	problems					217:224	the problems	213:224	the problems encountered in clinical practice	213:257	Large, deep, complex, and severe tissue defects and deformities of the face are the problems encountered in clinical practice.
34294283	1	50	theme	Large	133:137	arg1	deformities					185:195	deformities	185:195	deformities	185:195	Large, deep, complex, and severe tissue defects and deformities of the face are the problems encountered in clinical practice.
34294283	7	51	theme	vascularized	1119:1130	arg1	faces					1161:1165	personalized, complex, and vascularized tissue-engineered anatomical faces	1092:1165	personalized, complex, and vascularized tissue-engineered anatomical faces	1092:1165	The hydrogels prepared using these three polysaccharides have great potential in the construction of personalized, complex, and vascularized tissue-engineered anatomical faces and provide a new strategy for autologous full face reconstruction.
34294283	3	52	theme	personalized	478:489	arg1	regeneration					498:509	personalized tissue regeneration	478:509	personalized tissue regeneration	478:509	3D bioprinting enables personalized tissue regeneration.
34294283	0	53	dep	large-scale	73:83	arg1	personalized					86:97	personalized	86:97	personalized	86:97	Agarose composite hydrogel and PVA sacrificial materials for bioprinting large-scale, personalized face-like with nutrient networks.
34294283	2	54	theme	Autologous	260:269	arg1	reconstruction					278:291	Autologous tissue reconstruction or allograft face transplantation	260:325	reconstruction	278:291	Autologous tissue reconstruction or allograft face transplantation has been adopted but has problems such as blood supply difficulties, collateral damage, immune rejection, and ethical disputes.
34294283	1	55	theme	deep	140:143	arg1	defects					173:179	tissue defects	166:179	tissue defects	166:179	Large, deep, complex, and severe tissue defects and deformities of the face are the problems encountered in clinical practice.
34294283	1	55	theme	deep	140:143	arg1	problems					217:224	the problems	213:224	the problems encountered in clinical practice	213:257	Large, deep, complex, and severe tissue defects and deformities of the face are the problems encountered in clinical practice.
34294283	1	55	theme	deep	140:143	arg1	deformities					185:195	deformities	185:195	deformities	185:195	Large, deep, complex, and severe tissue defects and deformities of the face are the problems encountered in clinical practice.
34294283	0	56	theme	large-scale	73:83	arg1	face-like					99:107	large-scale, personalized face-like	73:107	large-scale, personalized face-like with nutrient networks	73:130	Agarose composite hydrogel and PVA sacrificial materials for bioprinting large-scale, personalized face-like with nutrient networks.
34294283	3	57	theme	3D	455:456	arg1	bioprinting					458:468	3D bioprinting	455:468	3D bioprinting	455:468	3D bioprinting enables personalized tissue regeneration.
34294283	2	58	theme	blood	369:373	arg1	difficulties					382:393	blood supply difficulties	369:393	blood supply difficulties	369:393	Autologous tissue reconstruction or allograft face transplantation has been adopted but has problems such as blood supply difficulties, collateral damage, immune rejection, and ethical disputes.
34294283	6	59	theme	blood	962:966	arg1	structure					980:988	a blood vessel-like structure	960:988	a blood vessel-like structure	960:988	The nutrient network gradually formed a blood vessel-like structure.
34294283	7	60	theme	new	1181:1183	arg1	strategy					1185:1192	a new strategy	1179:1192	a new strategy for autologous full face reconstruction	1179:1232	The hydrogels prepared using these three polysaccharides have great potential in the construction of personalized, complex, and vascularized tissue-engineered anatomical faces and provide a new strategy for autologous full face reconstruction.
34294283	4	61	contain	have	602:605	arg2	shape					612:616	shape	612:616	shape	612:616	However, simple hydrogels are prone to collapse during printing, are limited in size, and have poor shape and structure.
34294283	4	61	contain	have	602:605	arg2	structure					622:630	structure	622:630	structure	622:630	However, simple hydrogels are prone to collapse during printing, are limited in size, and have poor shape and structure.
34294283	4	61	contain	have	602:605	arg1	hydrogels					528:536	simple hydrogels	521:536	simple hydrogels	521:536	However, simple hydrogels are prone to collapse during printing, are limited in size, and have poor shape and structure.
34294283	1	62	theme	complex	146:152	arg1	defects					173:179	tissue defects	166:179	tissue defects	166:179	Large, deep, complex, and severe tissue defects and deformities of the face are the problems encountered in clinical practice.
34294283	1	62	theme	complex	146:152	arg1	problems					217:224	the problems	213:224	the problems encountered in clinical practice	213:257	Large, deep, complex, and severe tissue defects and deformities of the face are the problems encountered in clinical practice.
34294283	1	62	theme	complex	146:152	arg1	deformities					185:195	deformities	185:195	deformities	185:195	Large, deep, complex, and severe tissue defects and deformities of the face are the problems encountered in clinical practice.
34294283	4	63	theme	poor	607:610	arg1	shape					612:616	shape	612:616	shape	612:616	However, simple hydrogels are prone to collapse during printing, are limited in size, and have poor shape and structure.
33434856	5	0	theme	galacturonic	723:734	arg1	acid					736:739	galacturonic acid	723:739	galacturonic acid	723:739	H.syriacus petroleum ether extract (PEE) showed a high percentage of palmitic and oleic acids while its mucilage demonstrated high glucosamine and galacturonic acid.
33434856	8	1	theme	electron	1062:1069	arg1	microscopy					1071:1080	scanning electron microscopy	1053:1080	scanning electron microscopy	1053:1080	Morphological characteristics were evaluated using scanning electron microscopy.
33434856	11	2	theme	healing	1279:1285	arg1	ability					1287:1293	The wound healing ability	1269:1293	The wound healing ability	1269:1293	The wound healing ability was clearly proved by the clinical acceleration, histopathological examination, and modulation of correlated inflammatory parameters as tumor necrosis factor in addition to vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation.
33434856	9	3	theme	composite	1094:1102	arg1	sponge					1104:1109	H.syriacus composite sponge	1083:1109	H.syriacus composite sponge of mucilage: chitosan (1:5)	1083:1137	H.syriacus composite sponge of mucilage: chitosan (1:5) was loaded with three different concentrations of PEE.
33434856	10	4	theme	excision	1247:1254	arg1	model					1262:1266	excision wound model	1247:1266	excision wound model	1247:1266	Medicated formulations were assessed in rat model of excision wound model.
33434856	5	5	theme	oleic	658:662	arg1	acids					664:668	palmitic and oleic acids	645:668	palmitic and oleic acids	645:668	H.syriacus petroleum ether extract (PEE) showed a high percentage of palmitic and oleic acids while its mucilage demonstrated high glucosamine and galacturonic acid.
33434856	8	6	theme	scanning	1053:1060	arg1	microscopy					1071:1080	scanning electron microscopy	1053:1080	scanning electron microscopy	1053:1080	Morphological characteristics were evaluated using scanning electron microscopy.
33434856	7	7	theme	utilized	971:978	arg1	sponges					980:986	the utilized sponges	967:986	the utilized sponges' ingredients	967:999	Fourier-transformed infrared spectroscopy investigated the chemical interaction between the utilized sponges' ingredients.
33434856	0	8	theme	lipoidal	84:91	arg1	matter					93:98	lipoidal matter	84:98	lipoidal matter	84:98	In-vivo wound healing activity of a novel composite sponge loaded with mucilage and lipoidal matter of Hibiscus species.
33434856	11	9	theme	single-dose	1599:1609	arg1	preparation					1611:1621	single-dose preparation	1599:1621	single-dose preparation	1599:1621	The wound healing ability was clearly proved by the clinical acceleration, histopathological examination, and modulation of correlated inflammatory parameters as tumor necrosis factor in addition to vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation.
33434856	11	10	theme	excision	1577:1584	arg1	wounds					1586:1591	excision wounds	1577:1591	excision wounds using single-dose preparation	1577:1621	The wound healing ability was clearly proved by the clinical acceleration, histopathological examination, and modulation of correlated inflammatory parameters as tumor necrosis factor in addition to vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation.
33434856	4	11	theme	wound	552:556	arg1	healing					558:564	its wound healing	548:564	its wound healing activity	548:573	H.syriacus was the plant of choice to be incorporated in a new drug delivery system and evaluated for its wound healing activity.
33434856	11	12	theme	vascular	1468:1475	arg1	factor					1496:1501	vascular endothelial growth factor	1468:1501	vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation	1468:1621	The wound healing ability was clearly proved by the clinical acceleration, histopathological examination, and modulation of correlated inflammatory parameters as tumor necrosis factor in addition to vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation.
33434856	8	13	theme	Morphological	1002:1014	arg1	characteristics					1016:1030	Morphological characteristics	1002:1030	Morphological characteristics	1002:1030	Morphological characteristics were evaluated using scanning electron microscopy.
33434856	9	14	theme	mucilage	1114:1121	arg1	sponge					1104:1109	H.syriacus composite sponge	1083:1109	H.syriacus composite sponge of mucilage: chitosan (1:5)	1083:1137	H.syriacus composite sponge of mucilage: chitosan (1:5) was loaded with three different concentrations of PEE.
33434856	10	15	theme	rat	1234:1236	arg1	model					1238:1242	rat model	1234:1242	rat model of excision wound model	1234:1266	Medicated formulations were assessed in rat model of excision wound model.
33434856	10	16	theme	wound	1256:1260	arg1	model					1262:1266	excision wound model	1247:1266	excision wound model	1247:1266	Medicated formulations were assessed in rat model of excision wound model.
33434856	6	17	theme	various	863:869	arg1	ratios					871:876	various ratios	863:876	various ratios	863:876	It was selected to be formulated and pharmaceutically evaluated into three different composite sponges using chitosan in various ratios.
33434856	5	18	theme	petroleum	587:595	arg1	extract					603:609	H.syriacus petroleum ether extract	576:609	H.syriacus petroleum ether extract (PEE)	576:615	H.syriacus petroleum ether extract (PEE) showed a high percentage of palmitic and oleic acids while its mucilage demonstrated high glucosamine and galacturonic acid.
33434856	5	18	theme	petroleum	587:595	arg1	PEE					612:614	PEE	612:614	PEE	612:614	H.syriacus petroleum ether extract (PEE) showed a high percentage of palmitic and oleic acids while its mucilage demonstrated high glucosamine and galacturonic acid.
33434856	5	19	theme	H.syriacus	576:585	arg1	extract					603:609	H.syriacus petroleum ether extract	576:609	H.syriacus petroleum ether extract (PEE)	576:615	H.syriacus petroleum ether extract (PEE) showed a high percentage of palmitic and oleic acids while its mucilage demonstrated high glucosamine and galacturonic acid.
33434856	5	19	theme	H.syriacus	576:585	arg1	PEE					612:614	PEE	612:614	PEE	612:614	H.syriacus petroleum ether extract (PEE) showed a high percentage of palmitic and oleic acids while its mucilage demonstrated high glucosamine and galacturonic acid.
33434856	11	20	theme	correlated	1393:1402	arg1	parameters					1417:1426	correlated inflammatory parameters	1393:1426	correlated inflammatory parameters as tumor necrosis factor in addition to vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation	1393:1621	The wound healing ability was clearly proved by the clinical acceleration, histopathological examination, and modulation of correlated inflammatory parameters as tumor necrosis factor in addition to vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation.
33434856	5	21	theme	high	702:705	arg1	glucosamine					707:717	high glucosamine	702:717	high glucosamine	702:717	H.syriacus petroleum ether extract (PEE) showed a high percentage of palmitic and oleic acids while its mucilage demonstrated high glucosamine and galacturonic acid.
33434856	5	22	theme	ether	597:601	arg1	extract					603:609	H.syriacus petroleum ether extract	576:609	H.syriacus petroleum ether extract (PEE)	576:615	H.syriacus petroleum ether extract (PEE) showed a high percentage of palmitic and oleic acids while its mucilage demonstrated high glucosamine and galacturonic acid.
33434856	5	22	theme	ether	597:601	arg1	PEE					612:614	PEE	612:614	PEE	612:614	H.syriacus petroleum ether extract (PEE) showed a high percentage of palmitic and oleic acids while its mucilage demonstrated high glucosamine and galacturonic acid.
33434856	7	23	theme	chemical	938:945	arg1	interaction					947:957	the chemical interaction	934:957	the chemical interaction between the utilized sponges' ingredients	934:999	Fourier-transformed infrared spectroscopy investigated the chemical interaction between the utilized sponges' ingredients.
33434856	0	24	theme	In-vivo	0:6	arg1	healing					14:20	In-vivo wound healing	0:20	In-vivo wound healing activity of a novel composite sponge	0:57	In-vivo wound healing activity of a novel composite sponge loaded with mucilage and lipoidal matter of Hibiscus species.
33434856	11	25	theme	endothelial	1477:1487	arg1	factor					1496:1501	vascular endothelial growth factor	1468:1501	vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation	1468:1621	The wound healing ability was clearly proved by the clinical acceleration, histopathological examination, and modulation of correlated inflammatory parameters as tumor necrosis factor in addition to vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation.
33434856	0	26	theme	wound	8:12	arg1	healing					14:20	In-vivo wound healing	0:20	In-vivo wound healing activity of a novel composite sponge	0:57	In-vivo wound healing activity of a novel composite sponge loaded with mucilage and lipoidal matter of Hibiscus species.
33434856	7	27	dep	sponges	980:986	arg1	ingredients					989:999	ingredients	989:999	the utilized sponges' ingredients	967:999	Fourier-transformed infrared spectroscopy investigated the chemical interaction between the utilized sponges' ingredients.
33434856	11	28	theme	clinical	1321:1328	arg1	acceleration					1330:1341	the clinical acceleration	1317:1341	the clinical acceleration	1317:1341	The wound healing ability was clearly proved by the clinical acceleration, histopathological examination, and modulation of correlated inflammatory parameters as tumor necrosis factor in addition to vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation.
33434856	11	29	theme	growth	1489:1494	arg1	factor					1496:1501	vascular endothelial growth factor	1468:1501	vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation	1468:1621	The wound healing ability was clearly proved by the clinical acceleration, histopathological examination, and modulation of correlated inflammatory parameters as tumor necrosis factor in addition to vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation.
33434856	3	30	theme	species	347:353	arg1	extracts					355:362	All the tested species extracts	332:362	All the tested species extracts	332:362	All the tested species extracts were assessed for their viability percentage using the water-soluble tetrazolium.
33434856	4	31	theme	delivery	514:521	arg1	system					523:528	a new drug delivery system	503:528	a new drug delivery system	503:528	H.syriacus was the plant of choice to be incorporated in a new drug delivery system and evaluated for its wound healing activity.
33434856	11	32	theme	wounds	1586:1591	arg1	management					1563:1572	the management	1559:1572	the management of excision wounds using single-dose preparation	1559:1621	The wound healing ability was clearly proved by the clinical acceleration, histopathological examination, and modulation of correlated inflammatory parameters as tumor necrosis factor in addition to vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation.
33434856	2	33	dep	species	234:240	arg1	leaves					243:248	leaves	243:248	leaves	243:248	In this study, several Hibiscus species (leaves) were extracted with petroleum ether, methanol, and their mucilage was separated.
33434856	4	34	theme	drug	509:512	arg1	system					523:528	a new drug delivery system	503:528	a new drug delivery system	503:528	H.syriacus was the plant of choice to be incorporated in a new drug delivery system and evaluated for its wound healing activity.
33434856	0	35	theme	species	112:118	arg1	mucilage					71:78	mucilage	71:78	mucilage	71:78	In-vivo wound healing activity of a novel composite sponge loaded with mucilage and lipoidal matter of Hibiscus species.
33434856	0	35	theme	species	112:118	arg1	matter					93:98	lipoidal matter	84:98	lipoidal matter	84:98	In-vivo wound healing activity of a novel composite sponge loaded with mucilage and lipoidal matter of Hibiscus species.
33434856	2	36	theme	petroleum	271:279	arg1	ether					281:285	petroleum ether	271:285	petroleum ether	271:285	In this study, several Hibiscus species (leaves) were extracted with petroleum ether, methanol, and their mucilage was separated.
33434856	1	37	theme	wound	179:183	arg1	process					193:199	the natural wound healing process	167:199	the natural wound healing process	167:199	Many researches have been undergone to hasten the natural wound healing process.
33434856	0	38	theme	healing	14:20	arg1	activity					22:29	In-vivo wound healing activity	0:29	In-vivo wound healing activity of a novel composite sponge	0:57	In-vivo wound healing activity of a novel composite sponge loaded with mucilage and lipoidal matter of Hibiscus species.
33434856	0	39	theme	Hibiscus	103:110	arg1	species					112:118	Hibiscus species	103:118	Hibiscus species	103:118	In-vivo wound healing activity of a novel composite sponge loaded with mucilage and lipoidal matter of Hibiscus species.
33434856	11	40	theme	promising	1516:1524	arg1	candidate					1535:1543	a promising valuable candidate	1514:1543	a promising valuable candidate that supports the management of excision wounds using single-dose preparation	1514:1621	The wound healing ability was clearly proved by the clinical acceleration, histopathological examination, and modulation of correlated inflammatory parameters as tumor necrosis factor in addition to vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation.
33434856	1	41	theme	healing	185:191	arg1	process					193:199	the natural wound healing process	167:199	the natural wound healing process	167:199	Many researches have been undergone to hasten the natural wound healing process.
33434856	11	42	theme	inflammatory	1404:1415	arg1	parameters					1417:1426	correlated inflammatory parameters	1393:1426	correlated inflammatory parameters as tumor necrosis factor in addition to vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation	1393:1621	The wound healing ability was clearly proved by the clinical acceleration, histopathological examination, and modulation of correlated inflammatory parameters as tumor necrosis factor in addition to vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation.
33434856	5	43	theme	acids	664:668	arg1	percentage					631:640	a high percentage	624:640	a high percentage of palmitic and oleic acids	624:668	H.syriacus petroleum ether extract (PEE) showed a high percentage of palmitic and oleic acids while its mucilage demonstrated high glucosamine and galacturonic acid.
33434856	3	44	theme	viability	388:396	arg1	percentage					398:407	their viability percentage	382:407	their viability percentage	382:407	All the tested species extracts were assessed for their viability percentage using the water-soluble tetrazolium.
33434856	11	45	theme	parameters	1417:1426	arg1	acceleration					1330:1341	the clinical acceleration	1317:1341	the clinical acceleration	1317:1341	The wound healing ability was clearly proved by the clinical acceleration, histopathological examination, and modulation of correlated inflammatory parameters as tumor necrosis factor in addition to vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation.
33434856	11	45	theme	parameters	1417:1426	arg1	examination					1362:1372	histopathological examination	1344:1372	histopathological examination	1344:1372	The wound healing ability was clearly proved by the clinical acceleration, histopathological examination, and modulation of correlated inflammatory parameters as tumor necrosis factor in addition to vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation.
33434856	11	45	theme	parameters	1417:1426	arg1	modulation					1379:1388	modulation	1379:1388	modulation of correlated inflammatory parameters as tumor necrosis factor in addition to vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation	1379:1621	The wound healing ability was clearly proved by the clinical acceleration, histopathological examination, and modulation of correlated inflammatory parameters as tumor necrosis factor in addition to vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation.
33434856	10	46	theme	Medicated	1194:1202	arg1	formulations					1204:1215	Medicated formulations	1194:1215	Medicated formulations	1194:1215	Medicated formulations were assessed in rat model of excision wound model.
33434856	6	47	theme	composite	827:835	arg1	sponges					837:843	three different composite sponges	811:843	three different composite sponges using chitosan in various ratios	811:876	It was selected to be formulated and pharmaceutically evaluated into three different composite sponges using chitosan in various ratios.
33434856	3	48	theme	water-soluble	419:431	arg1	tetrazolium					433:443	the water-soluble tetrazolium	415:443	the water-soluble tetrazolium	415:443	All the tested species extracts were assessed for their viability percentage using the water-soluble tetrazolium.
33434856	0	49	theme	composite	42:50	arg1	sponge					52:57	a novel composite sponge	34:57	a novel composite sponge	34:57	In-vivo wound healing activity of a novel composite sponge loaded with mucilage and lipoidal matter of Hibiscus species.
33434856	5	50	theme	high	626:629	arg1	percentage					631:640	a high percentage	624:640	a high percentage of palmitic and oleic acids	624:668	H.syriacus petroleum ether extract (PEE) showed a high percentage of palmitic and oleic acids while its mucilage demonstrated high glucosamine and galacturonic acid.
33434856	6	51	theme	different	817:825	arg1	sponges					837:843	three different composite sponges	811:843	three different composite sponges using chitosan in various ratios	811:876	It was selected to be formulated and pharmaceutically evaluated into three different composite sponges using chitosan in various ratios.
33434856	1	52	theme	natural	171:177	arg1	process					193:199	the natural wound healing process	167:199	the natural wound healing process	167:199	Many researches have been undergone to hasten the natural wound healing process.
33434856	0	53	theme	novel	36:40	arg1	sponge					52:57	a novel composite sponge	34:57	a novel composite sponge	34:57	In-vivo wound healing activity of a novel composite sponge loaded with mucilage and lipoidal matter of Hibiscus species.
33434856	4	54	theme	new	505:507	arg1	system					523:528	a new drug delivery system	503:528	a new drug delivery system	503:528	H.syriacus was the plant of choice to be incorporated in a new drug delivery system and evaluated for its wound healing activity.
33434856	11	55	theme	histopathological	1344:1360	arg1	examination					1362:1372	histopathological examination	1344:1372	histopathological examination	1344:1372	The wound healing ability was clearly proved by the clinical acceleration, histopathological examination, and modulation of correlated inflammatory parameters as tumor necrosis factor in addition to vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation.
33434856	9	56	theme	different	1161:1169	arg1	concentrations					1171:1184	three different concentrations	1155:1184	three different concentrations of PEE	1155:1191	H.syriacus composite sponge of mucilage: chitosan (1:5) was loaded with three different concentrations of PEE.
33434856	7	57	theme	infrared	899:906	arg1	spectroscopy					908:919	Fourier-transformed infrared spectroscopy	879:919	Fourier-transformed infrared spectroscopy	879:919	Fourier-transformed infrared spectroscopy investigated the chemical interaction between the utilized sponges' ingredients.
33434856	11	58	theme	wound	1273:1277	arg1	healing					1279:1285	The wound healing	1269:1285	The wound healing ability	1269:1293	The wound healing ability was clearly proved by the clinical acceleration, histopathological examination, and modulation of correlated inflammatory parameters as tumor necrosis factor in addition to vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation.
33434856	9	59	dep	sponge	1104:1109	arg1	chitosan					1124:1131	chitosan (1:5)	1124:1137	H.syriacus composite sponge of mucilage: chitosan (1:5)	1083:1137	H.syriacus composite sponge of mucilage: chitosan (1:5) was loaded with three different concentrations of PEE.
33434856	11	60	theme	tumor	1431:1435	arg1	factor					1446:1451	tumor necrosis factor	1431:1451	tumor necrosis factor in addition to vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation	1431:1621	The wound healing ability was clearly proved by the clinical acceleration, histopathological examination, and modulation of correlated inflammatory parameters as tumor necrosis factor in addition to vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation.
33434856	5	61	theme	palmitic	645:652	arg1	acids					664:668	palmitic and oleic acids	645:668	palmitic and oleic acids	645:668	H.syriacus petroleum ether extract (PEE) showed a high percentage of palmitic and oleic acids while its mucilage demonstrated high glucosamine and galacturonic acid.
33434856	1	62	theme	Many	121:124	arg1	researches					126:135	Many researches	121:135	Many researches	121:135	Many researches have been undergone to hasten the natural wound healing process.
33434856	10	63	theme	model	1262:1266	arg1	model					1238:1242	rat model	1234:1242	rat model of excision wound model	1234:1266	Medicated formulations were assessed in rat model of excision wound model.
33434856	4	64	theme	healing	558:564	arg1	activity					566:573	its wound healing activity	548:573	its wound healing activity	548:573	H.syriacus was the plant of choice to be incorporated in a new drug delivery system and evaluated for its wound healing activity.
33434856	11	65	theme	necrosis	1437:1444	arg1	factor					1446:1451	tumor necrosis factor	1431:1451	tumor necrosis factor in addition to vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation	1431:1621	The wound healing ability was clearly proved by the clinical acceleration, histopathological examination, and modulation of correlated inflammatory parameters as tumor necrosis factor in addition to vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation.
33434856	2	66	theme	Hibiscus	225:232	arg1	species					234:240	several Hibiscus species	217:240	several Hibiscus species (leaves)	217:249	In this study, several Hibiscus species (leaves) were extracted with petroleum ether, methanol, and their mucilage was separated.
33434856	9	67	theme	PEE	1189:1191	arg1	concentrations					1171:1184	three different concentrations	1155:1184	three different concentrations of PEE	1155:1191	H.syriacus composite sponge of mucilage: chitosan (1:5) was loaded with three different concentrations of PEE.
33434856	7	68	theme	Fourier-transformed	879:897	arg1	spectroscopy					908:919	Fourier-transformed infrared spectroscopy	879:919	Fourier-transformed infrared spectroscopy	879:919	Fourier-transformed infrared spectroscopy investigated the chemical interaction between the utilized sponges' ingredients.
33434856	0	69	theme	sponge	52:57	arg1	activity					22:29	In-vivo wound healing activity	0:29	In-vivo wound healing activity of a novel composite sponge	0:57	In-vivo wound healing activity of a novel composite sponge loaded with mucilage and lipoidal matter of Hibiscus species.
33434856	4	70	theme	choice	474:479	arg1	plant					465:469	the plant	461:469	the plant of choice	461:479	H.syriacus was the plant of choice to be incorporated in a new drug delivery system and evaluated for its wound healing activity.
33434856	2	71	theme	several	217:223	arg1	species					234:240	several Hibiscus species	217:240	several Hibiscus species (leaves)	217:249	In this study, several Hibiscus species (leaves) were extracted with petroleum ether, methanol, and their mucilage was separated.
33434856	11	72	theme	valuable	1526:1533	arg1	candidate					1535:1543	a promising valuable candidate	1514:1543	a promising valuable candidate that supports the management of excision wounds using single-dose preparation	1514:1621	The wound healing ability was clearly proved by the clinical acceleration, histopathological examination, and modulation of correlated inflammatory parameters as tumor necrosis factor in addition to vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation.
33434856	3	73	theme	tested	340:345	arg1	extracts					355:362	All the tested species extracts	332:362	All the tested species extracts	332:362	All the tested species extracts were assessed for their viability percentage using the water-soluble tetrazolium.
33434856	9	74	theme	H.syriacus	1083:1092	arg1	sponge					1104:1109	H.syriacus composite sponge	1083:1109	H.syriacus composite sponge of mucilage: chitosan (1:5)	1083:1137	H.syriacus composite sponge of mucilage: chitosan (1:5) was loaded with three different concentrations of PEE.
34866441	0	0	theme	Serous	79:84	arg1	Carcinomas					108:117	Serous Tubal Intraepithelial Carcinomas	79:117	Serous Tubal Intraepithelial Carcinomas	79:117	Multi-modal Profiling of the Extracellular Matrix of Human Fallopian Tubes and Serous Tubal Intraepithelial Carcinomas.
34866441	8	1	theme	changes	1469:1475	arg1	picture					1402:1408	an in-depth picture	1390:1408	an in-depth picture of the ECM in the benign fallopian tube and identified ECM changes that accompany STIC formation	1390:1505	Our results provide an in-depth picture of the ECM in the benign fallopian tube and identified ECM changes that accompany STIC formation.
34866441	4	2	theme	published	664:672	arg1	datasets					684:691	published proteomic datasets	664:691	published proteomic datasets that identified 190 ECM proteins	664:724	The ECM composition of benign human fallopian tube was first defined from a meta-analysis of published proteomic datasets that identified 190 ECM proteins.
34866441	8	3	from	changes	1469:1475	arg1	tube					1445:1448	the benign fallopian tube	1424:1448	the benign fallopian tube	1424:1448	Our results provide an in-depth picture of the ECM in the benign fallopian tube and identified ECM changes that accompany STIC formation.
34866441	5	4	theme	de	745:746	arg1	proteomics					753:762	de novo proteomics	745:762	de novo proteomics	745:762	We then conducted de novo proteomics using ECM enrichment and identified 88 proteins, 7 of which were not identified in prior studies (COL2A1, COL4A5, COL16A1, elastin, LAMA5, annexin A2, and PAI1).
34866441	7	5	from	versican	1351:1358	arg1	STICs					1363:1367	STICs	1363:1367	STICs	1363:1367	Quantification revealed an increase in stromal fibronectin and a decrease in epithelial versican in STICs.
34866441	8	6	from	tube	1445:1448	arg1	picture					1402:1408	an in-depth picture	1390:1408	an in-depth picture of the ECM in the benign fallopian tube and identified ECM changes that accompany STIC formation	1390:1505	Our results provide an in-depth picture of the ECM in the benign fallopian tube and identified ECM changes that accompany STIC formation.
34866441	1	7	theme	fallopian	165:173	arg1	tube					175:178	the fallopian tube	161:178	the fallopian tube	161:178	Recent evidence supports the fimbriae of the fallopian tube as one origin site for high-grade serous ovarian cancer (HGSOC).
34866441	2	8	from	changes	300:306	arg1	microenvironment					315:330	the microenvironment	311:330	the microenvironment	311:330	The progression of many solid tumors is accompanied by changes in the microenvironment, including alterations of the extracellular matrix (ECM).
34866441	0	9	theme	Intraepithelial	92:106	arg1	Carcinomas					108:117	Serous Tubal Intraepithelial Carcinomas	79:117	Serous Tubal Intraepithelial Carcinomas	79:117	Multi-modal Profiling of the Extracellular Matrix of Human Fallopian Tubes and Serous Tubal Intraepithelial Carcinomas.
34866441	9	10	theme	J	1509:1509	arg1	Cytochem					1521:1528	J Histochem Cytochem XX	1509:1531	J Histochem Cytochem XX: XXX-XXX, XXXX	1509:1546	(J Histochem Cytochem XX: XXX-XXX, XXXX).
34866441	6	11	with	patients	1221:1228	arg1	STICs					1256:1260	STICs	1256:1260	STICs	1256:1260	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	6	11	with	patients	1221:1228	arg1	conditions					1242:1251	benign conditions	1235:1251	benign conditions	1235:1251	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	2	12	theme	tumors	275:280	arg1	progression					249:259	The progression	245:259	The progression of many solid tumors	245:280	The progression of many solid tumors is accompanied by changes in the microenvironment, including alterations of the extracellular matrix (ECM).
34866441	0	13	theme	Tubal	86:90	arg1	Carcinomas					108:117	Serous Tubal Intraepithelial Carcinomas	79:117	Serous Tubal Intraepithelial Carcinomas	79:117	Multi-modal Profiling of the Extracellular Matrix of Human Fallopian Tubes and Serous Tubal Intraepithelial Carcinomas.
34866441	8	14	theme	ECM	1417:1419	arg1	picture					1402:1408	an in-depth picture	1390:1408	an in-depth picture of the ECM in the benign fallopian tube and identified ECM changes that accompany STIC formation	1390:1505	Our results provide an in-depth picture of the ECM in the benign fallopian tube and identified ECM changes that accompany STIC formation.
34866441	6	15	theme	hyaluronic	1138:1147	arg1	acid					1149:1152	hyaluronic acid	1138:1152	hyaluronic acid	1138:1152	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	6	15	theme	hyaluronic	1138:1147	arg1	collagens					1081:1089	type I, III, and IV collagens	1061:1089	type I, III, and IV collagens	1061:1089	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	2	16	theme	solid	269:273	arg1	tumors					275:280	many solid tumors	264:280	many solid tumors	264:280	The progression of many solid tumors is accompanied by changes in the microenvironment, including alterations of the extracellular matrix (ECM).
34866441	6	17	theme	components	1012:1021	arg1	localization					992:1003	localization	992:1003	localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid)	992:1153	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	6	17	theme	components	1012:1021	arg1	levels					981:986	the levels	977:986	the levels	977:986	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	3	18	theme	fallopian	458:466	arg1	tube					468:471	the benign fallopian tube	447:471	the benign fallopian tube	447:471	Therefore, we sought to determine the ECM composition of the benign fallopian tube and changes associated with serous tubal intraepithelial carcinomas (STICs), precursors of HGSOC.
34866441	9	19	dep	Cytochem	1521:1528	arg1	XXX-XXX					1534:1540	XXX-XXX	1534:1540	XXX-XXX	1534:1540	(J Histochem Cytochem XX: XXX-XXX, XXXX).
34866441	9	19	dep	Cytochem	1521:1528	arg1	XXXX					1543:1546	XXXX	1543:1546	XXXX	1543:1546	(J Histochem Cytochem XX: XXX-XXX, XXXX).
34866441	2	20	theme	many	264:267	arg1	tumors					275:280	many solid tumors	264:280	many solid tumors	264:280	The progression of many solid tumors is accompanied by changes in the microenvironment, including alterations of the extracellular matrix (ECM).
34866441	1	21	theme	tube	175:178	arg1	fimbriae					149:156	the fimbriae	145:156	the fimbriae of the fallopian tube	145:178	Recent evidence supports the fimbriae of the fallopian tube as one origin site for high-grade serous ovarian cancer (HGSOC).
34866441	0	22	theme	Carcinomas	108:117	arg1	Matrix					43:48	the Extracellular Matrix	25:48	the Extracellular Matrix of Human Fallopian Tubes and Serous Tubal Intraepithelial Carcinomas	25:117	Multi-modal Profiling of the Extracellular Matrix of Human Fallopian Tubes and Serous Tubal Intraepithelial Carcinomas.
34866441	7	23	theme	epithelial	1340:1349	arg1	versican					1351:1358	epithelial versican	1340:1358	epithelial versican in STICs	1340:1367	Quantification revealed an increase in stromal fibronectin and a decrease in epithelial versican in STICs.
34866441	6	24	theme	benign	1235:1240	arg1	conditions					1242:1251	benign conditions	1235:1251	benign conditions	1235:1251	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	6	25	from	patients	1221:1228	arg1	staining					1195:1202	multispectral immunohistochemical staining	1161:1202	multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs	1161:1260	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	6	25	from	patients	1221:1228	arg1	fimbriae					1207:1214	fimbriae	1207:1214	fimbriae from patients with benign conditions or STICs	1207:1260	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	3	26	theme	tubal	508:512	arg1	STICs					542:546	STICs	542:546	STICs	542:546	Therefore, we sought to determine the ECM composition of the benign fallopian tube and changes associated with serous tubal intraepithelial carcinomas (STICs), precursors of HGSOC.
34866441	3	26	theme	tubal	508:512	arg1	precursors					550:559	precursors	550:559	precursors of HGSOC	550:568	Therefore, we sought to determine the ECM composition of the benign fallopian tube and changes associated with serous tubal intraepithelial carcinomas (STICs), precursors of HGSOC.
34866441	3	26	theme	tubal	508:512	arg1	carcinomas					530:539	serous tubal intraepithelial carcinomas	501:539	serous tubal intraepithelial carcinomas (STICs)	501:547	Therefore, we sought to determine the ECM composition of the benign fallopian tube and changes associated with serous tubal intraepithelial carcinomas (STICs), precursors of HGSOC.
34866441	6	27	dep	models	1053:1058	arg1	fibronectin					1092:1102	fibronectin	1092:1102	fibronectin	1092:1102	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	6	27	dep	models	1053:1058	arg1	perlecan					1124:1131	perlecan	1124:1131	perlecan	1124:1131	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	6	27	dep	models	1053:1058	arg1	laminin					1105:1111	laminin	1105:1111	laminin	1105:1111	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	6	27	dep	models	1053:1058	arg1	versican					1114:1121	versican	1114:1121	versican	1114:1121	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	6	27	dep	models	1053:1058	arg1	acid					1149:1152	hyaluronic acid	1138:1152	hyaluronic acid	1138:1152	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	6	27	dep	models	1053:1058	arg1	collagens					1081:1089	type I, III, and IV collagens	1061:1089	type I, III, and IV collagens	1061:1089	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	4	28	theme	fallopian	607:615	arg1	tube					617:620	benign human fallopian tube	594:620	benign human fallopian tube	594:620	The ECM composition of benign human fallopian tube was first defined from a meta-analysis of published proteomic datasets that identified 190 ECM proteins.
34866441	4	29	theme	ECM	713:715	arg1	proteins					717:724	190 ECM proteins	709:724	190 ECM proteins	709:724	The ECM composition of benign human fallopian tube was first defined from a meta-analysis of published proteomic datasets that identified 190 ECM proteins.
34866441	6	30	theme	future	936:941	arg1	studies					952:958	future in vitro studies	936:958	future in vitro studies	936:958	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	2	31	theme	matrix	376:381	arg1	alterations					343:353	alterations	343:353	alterations of the extracellular matrix (ECM)	343:387	The progression of many solid tumors is accompanied by changes in the microenvironment, including alterations of the extracellular matrix (ECM).
34866441	0	32	theme	Multi-modal	0:10	arg1	Profiling					12:20	Multi-modal Profiling	0:20	Multi-modal Profiling of the Extracellular Matrix of Human Fallopian Tubes and Serous Tubal Intraepithelial Carcinomas.	0:118	Multi-modal Profiling of the Extracellular Matrix of Human Fallopian Tubes and Serous Tubal Intraepithelial Carcinomas.
34866441	8	33	theme	fallopian	1435:1443	arg1	tube					1445:1448	the benign fallopian tube	1424:1448	the benign fallopian tube	1424:1448	Our results provide an in-depth picture of the ECM in the benign fallopian tube and identified ECM changes that accompany STIC formation.
34866441	6	34	theme	type	1061:1064	arg1	fibronectin					1092:1102	fibronectin	1092:1102	fibronectin	1092:1102	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	6	34	theme	type	1061:1064	arg1	perlecan					1124:1131	perlecan	1124:1131	perlecan	1124:1131	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	6	34	theme	type	1061:1064	arg1	laminin					1105:1111	laminin	1105:1111	laminin	1105:1111	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	6	34	theme	type	1061:1064	arg1	versican					1114:1121	versican	1114:1121	versican	1114:1121	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	6	34	theme	type	1061:1064	arg1	acid					1149:1152	hyaluronic acid	1138:1152	hyaluronic acid	1138:1152	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	6	34	theme	type	1061:1064	arg1	collagens					1081:1089	type I, III, and IV collagens	1061:1089	type I, III, and IV collagens	1061:1089	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	1	35	theme	origin	187:192	arg1	site					194:197	one origin site	183:197	one origin site for high-grade serous ovarian cancer (HGSOC)	183:242	Recent evidence supports the fimbriae of the fallopian tube as one origin site for high-grade serous ovarian cancer (HGSOC).
34866441	8	36	theme	benign	1428:1433	arg1	tube					1445:1448	the benign fallopian tube	1424:1448	the benign fallopian tube	1424:1448	Our results provide an in-depth picture of the ECM in the benign fallopian tube and identified ECM changes that accompany STIC formation.
34866441	4	37	theme	human	601:605	arg1	tube					617:620	benign human fallopian tube	594:620	benign human fallopian tube	594:620	The ECM composition of benign human fallopian tube was first defined from a meta-analysis of published proteomic datasets that identified 190 ECM proteins.
34866441	5	38	dep	studies	853:859	arg1	PAI1					919:922	PAI1	919:922	PAI1	919:922	We then conducted de novo proteomics using ECM enrichment and identified 88 proteins, 7 of which were not identified in prior studies (COL2A1, COL4A5, COL16A1, elastin, LAMA5, annexin A2, and PAI1).
34866441	5	38	dep	studies	853:859	arg1	COL4A5					870:875	COL4A5	870:875	COL4A5	870:875	We then conducted de novo proteomics using ECM enrichment and identified 88 proteins, 7 of which were not identified in prior studies (COL2A1, COL4A5, COL16A1, elastin, LAMA5, annexin A2, and PAI1).
34866441	5	38	dep	studies	853:859	arg1	LAMA5					896:900	LAMA5	896:900	LAMA5	896:900	We then conducted de novo proteomics using ECM enrichment and identified 88 proteins, 7 of which were not identified in prior studies (COL2A1, COL4A5, COL16A1, elastin, LAMA5, annexin A2, and PAI1).
34866441	5	38	dep	studies	853:859	arg1	A2					911:912	annexin A2	903:912	annexin A2	903:912	We then conducted de novo proteomics using ECM enrichment and identified 88 proteins, 7 of which were not identified in prior studies (COL2A1, COL4A5, COL16A1, elastin, LAMA5, annexin A2, and PAI1).
34866441	5	38	dep	studies	853:859	arg1	studies					853:859	prior studies	847:859	prior studies (COL2A1, COL4A5, COL16A1, elastin, LAMA5, annexin A2, and PAI1)	847:923	We then conducted de novo proteomics using ECM enrichment and identified 88 proteins, 7 of which were not identified in prior studies (COL2A1, COL4A5, COL16A1, elastin, LAMA5, annexin A2, and PAI1).
34866441	5	38	dep	studies	853:859	arg1	COL16A1					878:884	COL16A1	878:884	COL16A1	878:884	We then conducted de novo proteomics using ECM enrichment and identified 88 proteins, 7 of which were not identified in prior studies (COL2A1, COL4A5, COL16A1, elastin, LAMA5, annexin A2, and PAI1).
34866441	5	38	dep	studies	853:859	arg1	elastin					887:893	elastin	887:893	elastin	887:893	We then conducted de novo proteomics using ECM enrichment and identified 88 proteins, 7 of which were not identified in prior studies (COL2A1, COL4A5, COL16A1, elastin, LAMA5, annexin A2, and PAI1).
34866441	5	38	dep	studies	853:859	arg1	COL2A1					862:867	COL2A1	862:867	COL2A1	862:867	We then conducted de novo proteomics using ECM enrichment and identified 88 proteins, 7 of which were not identified in prior studies (COL2A1, COL4A5, COL16A1, elastin, LAMA5, annexin A2, and PAI1).
34866441	6	39	dep	in	943:944	arg1	vitro					946:950	vitro	946:950	vitro	946:950	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	3	40	theme	serous	501:506	arg1	STICs					542:546	STICs	542:546	STICs	542:546	Therefore, we sought to determine the ECM composition of the benign fallopian tube and changes associated with serous tubal intraepithelial carcinomas (STICs), precursors of HGSOC.
34866441	3	40	theme	serous	501:506	arg1	precursors					550:559	precursors	550:559	precursors of HGSOC	550:568	Therefore, we sought to determine the ECM composition of the benign fallopian tube and changes associated with serous tubal intraepithelial carcinomas (STICs), precursors of HGSOC.
34866441	3	40	theme	serous	501:506	arg1	carcinomas					530:539	serous tubal intraepithelial carcinomas	501:539	serous tubal intraepithelial carcinomas (STICs)	501:547	Therefore, we sought to determine the ECM composition of the benign fallopian tube and changes associated with serous tubal intraepithelial carcinomas (STICs), precursors of HGSOC.
34866441	3	41	theme	changes	477:483	arg1	composition					432:442	the ECM composition	424:442	the ECM composition of the benign fallopian tube and changes associated with serous tubal intraepithelial carcinomas (STICs), precursors of HGSOC	424:568	Therefore, we sought to determine the ECM composition of the benign fallopian tube and changes associated with serous tubal intraepithelial carcinomas (STICs), precursors of HGSOC.
34866441	7	42	theme	stromal	1302:1308	arg1	fibronectin					1310:1320	stromal fibronectin	1302:1320	stromal fibronectin	1302:1320	Quantification revealed an increase in stromal fibronectin and a decrease in epithelial versican in STICs.
34866441	4	43	theme	benign	594:599	arg1	tube					617:620	benign human fallopian tube	594:620	benign human fallopian tube	594:620	The ECM composition of benign human fallopian tube was first defined from a meta-analysis of published proteomic datasets that identified 190 ECM proteins.
34866441	0	44	theme	Matrix	43:48	arg1	Profiling					12:20	Multi-modal Profiling	0:20	Multi-modal Profiling of the Extracellular Matrix of Human Fallopian Tubes and Serous Tubal Intraepithelial Carcinomas.	0:118	Multi-modal Profiling of the Extracellular Matrix of Human Fallopian Tubes and Serous Tubal Intraepithelial Carcinomas.
34866441	4	45	theme	proteomic	674:682	arg1	datasets					684:691	published proteomic datasets	664:691	published proteomic datasets that identified 190 ECM proteins	664:724	The ECM composition of benign human fallopian tube was first defined from a meta-analysis of published proteomic datasets that identified 190 ECM proteins.
34866441	4	46	theme	tube	617:620	arg1	composition					579:589	The ECM composition	571:589	The ECM composition of benign human fallopian tube	571:620	The ECM composition of benign human fallopian tube was first defined from a meta-analysis of published proteomic datasets that identified 190 ECM proteins.
34866441	3	47	theme	HGSOC	564:568	arg1	precursors					550:559	precursors	550:559	precursors of HGSOC	550:568	Therefore, we sought to determine the ECM composition of the benign fallopian tube and changes associated with serous tubal intraepithelial carcinomas (STICs), precursors of HGSOC.
34866441	3	47	theme	HGSOC	564:568	arg1	carcinomas					530:539	serous tubal intraepithelial carcinomas	501:539	serous tubal intraepithelial carcinomas (STICs)	501:547	Therefore, we sought to determine the ECM composition of the benign fallopian tube and changes associated with serous tubal intraepithelial carcinomas (STICs), precursors of HGSOC.
34866441	2	48	theme	extracellular	362:374	arg1	ECM					384:386	ECM	384:386	ECM	384:386	The progression of many solid tumors is accompanied by changes in the microenvironment, including alterations of the extracellular matrix (ECM).
34866441	2	48	theme	extracellular	362:374	arg1	matrix					376:381	the extracellular matrix	358:381	the extracellular matrix (ECM)	358:387	The progression of many solid tumors is accompanied by changes in the microenvironment, including alterations of the extracellular matrix (ECM).
34866441	1	49	theme	high-grade	203:212	arg1	HGSOC					237:241	HGSOC	237:241	HGSOC	237:241	Recent evidence supports the fimbriae of the fallopian tube as one origin site for high-grade serous ovarian cancer (HGSOC).
34866441	1	49	theme	high-grade	203:212	arg1	cancer					229:234	high-grade serous ovarian cancer	203:234	high-grade serous ovarian cancer (HGSOC)	203:242	Recent evidence supports the fimbriae of the fallopian tube as one origin site for high-grade serous ovarian cancer (HGSOC).
34866441	0	50	theme	Extracellular	29:41	arg1	Matrix					43:48	the Extracellular Matrix	25:48	the Extracellular Matrix of Human Fallopian Tubes and Serous Tubal Intraepithelial Carcinomas	25:117	Multi-modal Profiling of the Extracellular Matrix of Human Fallopian Tubes and Serous Tubal Intraepithelial Carcinomas.
34866441	8	51	theme	ECM	1465:1467	arg1	changes					1469:1475	identified ECM changes	1454:1475	identified ECM changes that accompany STIC formation	1454:1505	Our results provide an in-depth picture of the ECM in the benign fallopian tube and identified ECM changes that accompany STIC formation.
34866441	5	52	dep	de	745:746	arg1	novo					748:751	novo	748:751	novo	748:751	We then conducted de novo proteomics using ECM enrichment and identified 88 proteins, 7 of which were not identified in prior studies (COL2A1, COL4A5, COL16A1, elastin, LAMA5, annexin A2, and PAI1).
34866441	7	53	from	decrease	1328:1335	arg1	fibronectin					1310:1320	stromal fibronectin	1302:1320	stromal fibronectin	1302:1320	Quantification revealed an increase in stromal fibronectin and a decrease in epithelial versican in STICs.
34866441	7	53	from	decrease	1328:1335	arg1	versican					1351:1358	epithelial versican	1340:1358	epithelial versican in STICs	1340:1367	Quantification revealed an increase in stromal fibronectin and a decrease in epithelial versican in STICs.
34866441	1	54	theme	serous	214:219	arg1	HGSOC					237:241	HGSOC	237:241	HGSOC	237:241	Recent evidence supports the fimbriae of the fallopian tube as one origin site for high-grade serous ovarian cancer (HGSOC).
34866441	1	54	theme	serous	214:219	arg1	cancer					229:234	high-grade serous ovarian cancer	203:234	high-grade serous ovarian cancer (HGSOC)	203:242	Recent evidence supports the fimbriae of the fallopian tube as one origin site for high-grade serous ovarian cancer (HGSOC).
34866441	0	55	theme	Human	53:57	arg1	Tubes					69:73	Human Fallopian Tubes	53:73	Human Fallopian Tubes	53:73	Multi-modal Profiling of the Extracellular Matrix of Human Fallopian Tubes and Serous Tubal Intraepithelial Carcinomas.
34866441	8	56	theme	identified	1454:1463	arg1	changes					1469:1475	identified ECM changes	1454:1475	identified ECM changes that accompany STIC formation	1454:1505	Our results provide an in-depth picture of the ECM in the benign fallopian tube and identified ECM changes that accompany STIC formation.
34866441	6	57	theme	ECM	1008:1010	arg1	components					1012:1021	ECM components	1008:1021	ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid)	1008:1153	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	5	58	theme	annexin	903:909	arg1	studies					853:859	prior studies	847:859	prior studies (COL2A1, COL4A5, COL16A1, elastin, LAMA5, annexin A2, and PAI1)	847:923	We then conducted de novo proteomics using ECM enrichment and identified 88 proteins, 7 of which were not identified in prior studies (COL2A1, COL4A5, COL16A1, elastin, LAMA5, annexin A2, and PAI1).
34866441	5	58	theme	annexin	903:909	arg1	A2					911:912	annexin A2	903:912	annexin A2	903:912	We then conducted de novo proteomics using ECM enrichment and identified 88 proteins, 7 of which were not identified in prior studies (COL2A1, COL4A5, COL16A1, elastin, LAMA5, annexin A2, and PAI1).
34866441	6	59	theme	immunohistochemical	1175:1193	arg1	staining					1195:1202	multispectral immunohistochemical staining	1161:1202	multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs	1161:1260	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	1	60	theme	Recent	120:125	arg1	evidence					127:134	Recent evidence	120:134	Recent evidence	120:134	Recent evidence supports the fimbriae of the fallopian tube as one origin site for high-grade serous ovarian cancer (HGSOC).
34866441	5	61	theme	ECM	770:772	arg1	enrichment					774:783	ECM enrichment	770:783	ECM enrichment	770:783	We then conducted de novo proteomics using ECM enrichment and identified 88 proteins, 7 of which were not identified in prior studies (COL2A1, COL4A5, COL16A1, elastin, LAMA5, annexin A2, and PAI1).
34866441	8	62	from	ECM	1417:1419	arg1	tube					1445:1448	the benign fallopian tube	1424:1448	the benign fallopian tube	1424:1448	Our results provide an in-depth picture of the ECM in the benign fallopian tube and identified ECM changes that accompany STIC formation.
34866441	8	63	from	picture	1402:1408	arg1	tube					1445:1448	the benign fallopian tube	1424:1448	the benign fallopian tube	1424:1448	Our results provide an in-depth picture of the ECM in the benign fallopian tube and identified ECM changes that accompany STIC formation.
34866441	6	64	theme	in	943:944	arg1	studies					952:958	future in vitro studies	936:958	future in vitro studies	936:958	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	3	65	theme	tube	468:471	arg1	composition					432:442	the ECM composition	424:442	the ECM composition of the benign fallopian tube and changes associated with serous tubal intraepithelial carcinomas (STICs), precursors of HGSOC	424:568	Therefore, we sought to determine the ECM composition of the benign fallopian tube and changes associated with serous tubal intraepithelial carcinomas (STICs), precursors of HGSOC.
34866441	7	66	from	increase	1290:1297	arg1	fibronectin					1310:1320	stromal fibronectin	1302:1320	stromal fibronectin	1302:1320	Quantification revealed an increase in stromal fibronectin and a decrease in epithelial versican in STICs.
34866441	7	66	from	increase	1290:1297	arg1	versican					1351:1358	epithelial versican	1340:1358	epithelial versican in STICs	1340:1367	Quantification revealed an increase in stromal fibronectin and a decrease in epithelial versican in STICs.
34866441	6	67	from	staining	1195:1202	arg1	patients					1221:1228	patients	1221:1228	patients with benign conditions or STICs	1221:1260	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	4	68	theme	datasets	684:691	arg1	meta-analysis					647:659	a meta-analysis	645:659	a meta-analysis of published proteomic datasets that identified 190 ECM proteins	645:724	The ECM composition of benign human fallopian tube was first defined from a meta-analysis of published proteomic datasets that identified 190 ECM proteins.
34866441	6	69	theme	fimbriae	1207:1214	arg1	staining					1195:1202	multispectral immunohistochemical staining	1161:1202	multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs	1161:1260	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	1	70	theme	ovarian	221:227	arg1	HGSOC					237:241	HGSOC	237:241	HGSOC	237:241	Recent evidence supports the fimbriae of the fallopian tube as one origin site for high-grade serous ovarian cancer (HGSOC).
34866441	1	70	theme	ovarian	221:227	arg1	cancer					229:234	high-grade serous ovarian cancer	203:234	high-grade serous ovarian cancer (HGSOC)	203:242	Recent evidence supports the fimbriae of the fallopian tube as one origin site for high-grade serous ovarian cancer (HGSOC).
34866441	6	71	theme	multispectral	1161:1173	arg1	staining					1195:1202	multispectral immunohistochemical staining	1161:1202	multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs	1161:1260	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	3	72	theme	intraepithelial	514:528	arg1	STICs					542:546	STICs	542:546	STICs	542:546	Therefore, we sought to determine the ECM composition of the benign fallopian tube and changes associated with serous tubal intraepithelial carcinomas (STICs), precursors of HGSOC.
34866441	3	72	theme	intraepithelial	514:528	arg1	precursors					550:559	precursors	550:559	precursors of HGSOC	550:568	Therefore, we sought to determine the ECM composition of the benign fallopian tube and changes associated with serous tubal intraepithelial carcinomas (STICs), precursors of HGSOC.
34866441	3	72	theme	intraepithelial	514:528	arg1	carcinomas					530:539	serous tubal intraepithelial carcinomas	501:539	serous tubal intraepithelial carcinomas (STICs)	501:547	Therefore, we sought to determine the ECM composition of the benign fallopian tube and changes associated with serous tubal intraepithelial carcinomas (STICs), precursors of HGSOC.
34866441	3	73	theme	benign	451:456	arg1	tube					468:471	the benign fallopian tube	447:471	the benign fallopian tube	447:471	Therefore, we sought to determine the ECM composition of the benign fallopian tube and changes associated with serous tubal intraepithelial carcinomas (STICs), precursors of HGSOC.
34866441	6	74	theme	tissue-engineered	1035:1051	arg1	models					1053:1058	tissue-engineered models	1035:1058	tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid)	1035:1153	To enable future in vitro studies, we investigated the levels and localization of ECM components included in tissue-engineered models (type I, III, and IV collagens, fibronectin, laminin, versican, perlecan, and hyaluronic acid) using multispectral immunohistochemical staining of fimbriae from patients with benign conditions or STICs.
34866441	8	75	theme	STIC	1492:1495	arg1	formation					1497:1505	STIC formation	1492:1505	STIC formation	1492:1505	Our results provide an in-depth picture of the ECM in the benign fallopian tube and identified ECM changes that accompany STIC formation.
34866441	0	76	theme	Tubes	69:73	arg1	Matrix					43:48	the Extracellular Matrix	25:48	the Extracellular Matrix of Human Fallopian Tubes and Serous Tubal Intraepithelial Carcinomas	25:117	Multi-modal Profiling of the Extracellular Matrix of Human Fallopian Tubes and Serous Tubal Intraepithelial Carcinomas.
34866441	3	77	theme	ECM	428:430	arg1	composition					432:442	the ECM composition	424:442	the ECM composition of the benign fallopian tube and changes associated with serous tubal intraepithelial carcinomas (STICs), precursors of HGSOC	424:568	Therefore, we sought to determine the ECM composition of the benign fallopian tube and changes associated with serous tubal intraepithelial carcinomas (STICs), precursors of HGSOC.
34866441	9	78	theme	Histochem	1511:1519	arg1	Cytochem					1521:1528	J Histochem Cytochem XX	1509:1531	J Histochem Cytochem XX: XXX-XXX, XXXX	1509:1546	(J Histochem Cytochem XX: XXX-XXX, XXXX).
34866441	5	79	theme	proteins	803:810	arg1	7					813:813	7	813:813	7	813:813	We then conducted de novo proteomics using ECM enrichment and identified 88 proteins, 7 of which were not identified in prior studies (COL2A1, COL4A5, COL16A1, elastin, LAMA5, annexin A2, and PAI1).
34866441	5	79	theme	proteins	803:810	arg1	proteins					803:810	88 proteins	800:810	88 proteins	800:810	We then conducted de novo proteomics using ECM enrichment and identified 88 proteins, 7 of which were not identified in prior studies (COL2A1, COL4A5, COL16A1, elastin, LAMA5, annexin A2, and PAI1).
34866441	5	80	theme	prior	847:851	arg1	PAI1					919:922	PAI1	919:922	PAI1	919:922	We then conducted de novo proteomics using ECM enrichment and identified 88 proteins, 7 of which were not identified in prior studies (COL2A1, COL4A5, COL16A1, elastin, LAMA5, annexin A2, and PAI1).
34866441	5	80	theme	prior	847:851	arg1	COL4A5					870:875	COL4A5	870:875	COL4A5	870:875	We then conducted de novo proteomics using ECM enrichment and identified 88 proteins, 7 of which were not identified in prior studies (COL2A1, COL4A5, COL16A1, elastin, LAMA5, annexin A2, and PAI1).
34866441	5	80	theme	prior	847:851	arg1	LAMA5					896:900	LAMA5	896:900	LAMA5	896:900	We then conducted de novo proteomics using ECM enrichment and identified 88 proteins, 7 of which were not identified in prior studies (COL2A1, COL4A5, COL16A1, elastin, LAMA5, annexin A2, and PAI1).
34866441	5	80	theme	prior	847:851	arg1	A2					911:912	annexin A2	903:912	annexin A2	903:912	We then conducted de novo proteomics using ECM enrichment and identified 88 proteins, 7 of which were not identified in prior studies (COL2A1, COL4A5, COL16A1, elastin, LAMA5, annexin A2, and PAI1).
34866441	5	80	theme	prior	847:851	arg1	studies					853:859	prior studies	847:859	prior studies (COL2A1, COL4A5, COL16A1, elastin, LAMA5, annexin A2, and PAI1)	847:923	We then conducted de novo proteomics using ECM enrichment and identified 88 proteins, 7 of which were not identified in prior studies (COL2A1, COL4A5, COL16A1, elastin, LAMA5, annexin A2, and PAI1).
34866441	5	80	theme	prior	847:851	arg1	COL16A1					878:884	COL16A1	878:884	COL16A1	878:884	We then conducted de novo proteomics using ECM enrichment and identified 88 proteins, 7 of which were not identified in prior studies (COL2A1, COL4A5, COL16A1, elastin, LAMA5, annexin A2, and PAI1).
34866441	5	80	theme	prior	847:851	arg1	elastin					887:893	elastin	887:893	elastin	887:893	We then conducted de novo proteomics using ECM enrichment and identified 88 proteins, 7 of which were not identified in prior studies (COL2A1, COL4A5, COL16A1, elastin, LAMA5, annexin A2, and PAI1).
34866441	5	80	theme	prior	847:851	arg1	COL2A1					862:867	COL2A1	862:867	COL2A1	862:867	We then conducted de novo proteomics using ECM enrichment and identified 88 proteins, 7 of which were not identified in prior studies (COL2A1, COL4A5, COL16A1, elastin, LAMA5, annexin A2, and PAI1).
34866441	0	81	theme	Fallopian	59:67	arg1	Tubes					69:73	Human Fallopian Tubes	53:73	Human Fallopian Tubes	53:73	Multi-modal Profiling of the Extracellular Matrix of Human Fallopian Tubes and Serous Tubal Intraepithelial Carcinomas.
34866441	8	82	theme	in-depth	1393:1400	arg1	picture					1402:1408	an in-depth picture	1390:1408	an in-depth picture of the ECM in the benign fallopian tube and identified ECM changes that accompany STIC formation	1390:1505	Our results provide an in-depth picture of the ECM in the benign fallopian tube and identified ECM changes that accompany STIC formation.
34866441	4	83	theme	ECM	575:577	arg1	composition					579:589	The ECM composition	571:589	The ECM composition of benign human fallopian tube	571:620	The ECM composition of benign human fallopian tube was first defined from a meta-analysis of published proteomic datasets that identified 190 ECM proteins.
33479326	0	0	theme	multiple	86:93	arg1	sites					95:99	multiple sites	86:99	multiple sites in the United States	86:120	Metagenomic insights of the infant microbiome community structure and function across multiple sites in the United States.
33479326	4	1	theme	complete	926:933	arg1	capacity					935:942	a complete capacity	924:942	a complete capacity for HMOs utilization	924:963	Bifidobacterium were less abundant than generally expected and the species identified, including B. breve, B. longum and B. bifidum, had limited genetic capacity to metabolize human milk oligosaccharides (HMOs), while B. infantis strains with a complete capacity for HMOs utilization were found to be exceptionally rare.
33479326	4	2	theme	limited	818:824	arg1	capacity					834:841	limited genetic capacity	818:841	limited genetic capacity	818:841	Bifidobacterium were less abundant than generally expected and the species identified, including B. breve, B. longum and B. bifidum, had limited genetic capacity to metabolize human milk oligosaccharides (HMOs), while B. infantis strains with a complete capacity for HMOs utilization were found to be exceptionally rare.
33479326	4	3	theme	human	857:861	arg1	HMOs					886:889	HMOs	886:889	HMOs	886:889	Bifidobacterium were less abundant than generally expected and the species identified, including B. breve, B. longum and B. bifidum, had limited genetic capacity to metabolize human milk oligosaccharides (HMOs), while B. infantis strains with a complete capacity for HMOs utilization were found to be exceptionally rare.
33479326	4	3	theme	human	857:861	arg1	oligosaccharides					868:883	human milk oligosaccharides	857:883	human milk oligosaccharides (HMOs)	857:890	Bifidobacterium were less abundant than generally expected and the species identified, including B. breve, B. longum and B. bifidum, had limited genetic capacity to metabolize human milk oligosaccharides (HMOs), while B. infantis strains with a complete capacity for HMOs utilization were found to be exceptionally rare.
33479326	1	4	theme	immune	235:240	arg1	development					249:259	immune system development	235:259	immune system development	235:259	The gut microbiome plays an important role in early life, protecting newborns from enteric pathogens, promoting immune system development and providing key functions to the infant host.
33479326	5	5	from	widespread	1128:1137	arg1	microbiome					1173:1182	the contemporary US infant gut microbiome	1142:1182	the contemporary US infant gut microbiome	1142:1182	Considering microbiome composition and functional capacity, this survey revealed a previously unappreciated dysbiosis that is widespread in the contemporary US infant gut microbiome.
33479326	1	6	theme	important	151:159	arg1	role					161:164	an important role	148:164	an important role	148:164	The gut microbiome plays an important role in early life, protecting newborns from enteric pathogens, promoting immune system development and providing key functions to the infant host.
33479326	4	7	contain	had	814:816	arg2	capacity					834:841	limited genetic capacity	818:841	limited genetic capacity	818:841	Bifidobacterium were less abundant than generally expected and the species identified, including B. breve, B. longum and B. bifidum, had limited genetic capacity to metabolize human milk oligosaccharides (HMOs), while B. infantis strains with a complete capacity for HMOs utilization were found to be exceptionally rare.
33479326	4	7	contain	had	814:816	arg1	breve					781:785	B. breve	778:785	B. breve	778:785	Bifidobacterium were less abundant than generally expected and the species identified, including B. breve, B. longum and B. bifidum, had limited genetic capacity to metabolize human milk oligosaccharides (HMOs), while B. infantis strains with a complete capacity for HMOs utilization were found to be exceptionally rare.
33479326	4	7	contain	had	814:816	arg1	species					748:754	the species	744:754	the species	744:754	Bifidobacterium were less abundant than generally expected and the species identified, including B. breve, B. longum and B. bifidum, had limited genetic capacity to metabolize human milk oligosaccharides (HMOs), while B. infantis strains with a complete capacity for HMOs utilization were found to be exceptionally rare.
33479326	1	8	theme	system	242:247	arg1	development					249:259	immune system development	235:259	immune system development	235:259	The gut microbiome plays an important role in early life, protecting newborns from enteric pathogens, promoting immune system development and providing key functions to the infant host.
33479326	3	9	theme	age	666:668	arg1	independent					641:651	independent	641:651	independent	641:651	To address this gap, we performed a multi-state metagenomic survey and found high levels of bacteria associated with enteric inflammation (e.g. Escherichia, Klebsiella), antibiotic resistance genes, and signatures of dysbiosis, independent of location, age, and diet.
33479326	4	10	theme	genetic	826:832	arg1	capacity					834:841	limited genetic capacity	818:841	limited genetic capacity	818:841	Bifidobacterium were less abundant than generally expected and the species identified, including B. breve, B. longum and B. bifidum, had limited genetic capacity to metabolize human milk oligosaccharides (HMOs), while B. infantis strains with a complete capacity for HMOs utilization were found to be exceptionally rare.
33479326	3	11	theme	with enteric	525:536	arg1	inflammation					538:549	with enteric inflammation	525:549	with enteric inflammation	525:549	To address this gap, we performed a multi-state metagenomic survey and found high levels of bacteria associated with enteric inflammation (e.g. Escherichia, Klebsiella), antibiotic resistance genes, and signatures of dysbiosis, independent of location, age, and diet.
33479326	3	12	theme	multi-state	449:459	arg1	survey					473:478	a multi-state metagenomic survey	447:478	a multi-state metagenomic survey	447:478	To address this gap, we performed a multi-state metagenomic survey and found high levels of bacteria associated with enteric inflammation (e.g. Escherichia, Klebsiella), antibiotic resistance genes, and signatures of dysbiosis, independent of location, age, and diet.
33479326	0	13	theme	United	108:113	arg1	States					115:120	the United States	104:120	the United States	104:120	Metagenomic insights of the infant microbiome community structure and function across multiple sites in the United States.
33479326	5	14	theme	functional	1041:1050	arg1	capacity					1052:1059	functional capacity	1041:1059	functional capacity	1041:1059	Considering microbiome composition and functional capacity, this survey revealed a previously unappreciated dysbiosis that is widespread in the contemporary US infant gut microbiome.
33479326	4	15	theme	B.	899:900	arg1	strains					911:917	B. infantis strains	899:917	B. infantis strains with a complete capacity for HMOs utilization	899:963	Bifidobacterium were less abundant than generally expected and the species identified, including B. breve, B. longum and B. bifidum, had limited genetic capacity to metabolize human milk oligosaccharides (HMOs), while B. infantis strains with a complete capacity for HMOs utilization were found to be exceptionally rare.
33479326	3	16	theme	diet	675:678	arg1	independent					641:651	independent	641:651	independent	641:651	To address this gap, we performed a multi-state metagenomic survey and found high levels of bacteria associated with enteric inflammation (e.g. Escherichia, Klebsiella), antibiotic resistance genes, and signatures of dysbiosis, independent of location, age, and diet.
33479326	4	17	theme	B.	778:779	arg1	bifidum					805:811	B. bifidum	802:811	B. bifidum	802:811	Bifidobacterium were less abundant than generally expected and the species identified, including B. breve, B. longum and B. bifidum, had limited genetic capacity to metabolize human milk oligosaccharides (HMOs), while B. infantis strains with a complete capacity for HMOs utilization were found to be exceptionally rare.
33479326	4	17	theme	B.	778:779	arg1	breve					781:785	B. breve	778:785	B. breve	778:785	Bifidobacterium were less abundant than generally expected and the species identified, including B. breve, B. longum and B. bifidum, had limited genetic capacity to metabolize human milk oligosaccharides (HMOs), while B. infantis strains with a complete capacity for HMOs utilization were found to be exceptionally rare.
33479326	4	17	theme	B.	778:779	arg1	longum					791:796	B. longum	788:796	B. longum	788:796	Bifidobacterium were less abundant than generally expected and the species identified, including B. breve, B. longum and B. bifidum, had limited genetic capacity to metabolize human milk oligosaccharides (HMOs), while B. infantis strains with a complete capacity for HMOs utilization were found to be exceptionally rare.
33479326	3	18	theme	bacteria	505:512	arg1	levels					495:500	high levels	490:500	high levels	490:500	To address this gap, we performed a multi-state metagenomic survey and found high levels of bacteria associated with enteric inflammation (e.g. Escherichia, Klebsiella), antibiotic resistance genes, and signatures of dysbiosis, independent of location, age, and diet.
33479326	4	19	theme	infantis	902:909	arg1	strains					911:917	B. infantis strains	899:917	B. infantis strains with a complete capacity for HMOs utilization	899:963	Bifidobacterium were less abundant than generally expected and the species identified, including B. breve, B. longum and B. bifidum, had limited genetic capacity to metabolize human milk oligosaccharides (HMOs), while B. infantis strains with a complete capacity for HMOs utilization were found to be exceptionally rare.
33479326	1	20	theme	early	169:173	arg1	life					175:178	early life	169:178	early life	169:178	The gut microbiome plays an important role in early life, protecting newborns from enteric pathogens, promoting immune system development and providing key functions to the infant host.
33479326	0	21	theme	Metagenomic	0:10	arg1	insights					12:19	Metagenomic insights	0:19	Metagenomic insights of the infant	0:33	Metagenomic insights of the infant microbiome community structure and function across multiple sites in the United States.
33479326	5	22	theme	infant	1162:1167	arg1	microbiome					1173:1182	the contemporary US infant gut microbiome	1142:1182	the contemporary US infant gut microbiome	1142:1182	Considering microbiome composition and functional capacity, this survey revealed a previously unappreciated dysbiosis that is widespread in the contemporary US infant gut microbiome.
33479326	3	23	theme	independent	641:651	arg1	levels					495:500	high levels	490:500	high levels	490:500	To address this gap, we performed a multi-state metagenomic survey and found high levels of bacteria associated with enteric inflammation (e.g. Escherichia, Klebsiella), antibiotic resistance genes, and signatures of dysbiosis, independent of location, age, and diet.
33479326	1	24	theme	key	275:277	arg1	functions					279:287	key functions	275:287	key functions to the infant host	275:306	The gut microbiome plays an important role in early life, protecting newborns from enteric pathogens, promoting immune system development and providing key functions to the infant host.
33479326	5	25	from	microbiome	1173:1182	arg1	dysbiosis					1110:1118	a previously unappreciated dysbiosis	1083:1118	a previously unappreciated dysbiosis that is widespread in the contemporary US infant gut microbiome	1083:1182	Considering microbiome composition and functional capacity, this survey revealed a previously unappreciated dysbiosis that is widespread in the contemporary US infant gut microbiome.
33479326	5	25	from	microbiome	1173:1182	arg1	widespread					1128:1137	widespread	1128:1137	widespread	1128:1137	Considering microbiome composition and functional capacity, this survey revealed a previously unappreciated dysbiosis that is widespread in the contemporary US infant gut microbiome.
33479326	2	26	theme	microbiome	401:410	arg1	status					365:370	the status	361:370	the status of the US healthy infant gut microbiome	361:410	Currently, there are limited data to broadly assess the status of the US healthy infant gut microbiome.
33479326	5	27	theme	contemporary	1146:1157	arg1	microbiome					1173:1182	the contemporary US infant gut microbiome	1142:1182	the contemporary US infant gut microbiome	1142:1182	Considering microbiome composition and functional capacity, this survey revealed a previously unappreciated dysbiosis that is widespread in the contemporary US infant gut microbiome.
33479326	5	28	theme	microbiome	1014:1023	arg1	composition					1025:1035	microbiome composition	1014:1035	microbiome composition	1014:1035	Considering microbiome composition and functional capacity, this survey revealed a previously unappreciated dysbiosis that is widespread in the contemporary US infant gut microbiome.
33479326	1	29	dep	plays	142:146	arg1	protecting					181:190	protecting	181:190	protecting newborns from enteric pathogens	181:222	The gut microbiome plays an important role in early life, protecting newborns from enteric pathogens, promoting immune system development and providing key functions to the infant host.
33479326	1	29	dep	plays	142:146	arg1	providing					265:273	providing	265:273	providing key functions to the infant host	265:306	The gut microbiome plays an important role in early life, protecting newborns from enteric pathogens, promoting immune system development and providing key functions to the infant host.
33479326	1	29	dep	plays	142:146	arg1	promoting					225:233	promoting	225:233	promoting immune system development	225:259	The gut microbiome plays an important role in early life, protecting newborns from enteric pathogens, promoting immune system development and providing key functions to the infant host.
33479326	2	30	theme	gut	397:399	arg1	microbiome					401:410	the US healthy infant gut microbiome	375:410	the US healthy infant gut microbiome	375:410	Currently, there are limited data to broadly assess the status of the US healthy infant gut microbiome.
33479326	5	31	theme	unappreciated	1096:1108	arg1	dysbiosis					1110:1118	a previously unappreciated dysbiosis	1083:1118	a previously unappreciated dysbiosis that is widespread in the contemporary US infant gut microbiome	1083:1182	Considering microbiome composition and functional capacity, this survey revealed a previously unappreciated dysbiosis that is widespread in the contemporary US infant gut microbiome.
33479326	5	31	theme	unappreciated	1096:1108	arg1	widespread					1128:1137	widespread	1128:1137	widespread	1128:1137	Considering microbiome composition and functional capacity, this survey revealed a previously unappreciated dysbiosis that is widespread in the contemporary US infant gut microbiome.
33479326	3	32	theme	dysbiosis	630:638	arg1	genes					605:609	antibiotic resistance genes	583:609	antibiotic resistance genes	583:609	To address this gap, we performed a multi-state metagenomic survey and found high levels of bacteria associated with enteric inflammation (e.g. Escherichia, Klebsiella), antibiotic resistance genes, and signatures of dysbiosis, independent of location, age, and diet.
33479326	3	32	theme	dysbiosis	630:638	arg1	bacteria					505:512	bacteria	505:512	bacteria associated with enteric inflammation (e.g. Escherichia, Klebsiella)	505:580	To address this gap, we performed a multi-state metagenomic survey and found high levels of bacteria associated with enteric inflammation (e.g. Escherichia, Klebsiella), antibiotic resistance genes, and signatures of dysbiosis, independent of location, age, and diet.
33479326	3	32	theme	dysbiosis	630:638	arg1	signatures					616:625	signatures	616:625	signatures of dysbiosis	616:638	To address this gap, we performed a multi-state metagenomic survey and found high levels of bacteria associated with enteric inflammation (e.g. Escherichia, Klebsiella), antibiotic resistance genes, and signatures of dysbiosis, independent of location, age, and diet.
33479326	2	33	theme	healthy infant	382:395	arg1	microbiome					401:410	the US healthy infant gut microbiome	375:410	the US healthy infant gut microbiome	375:410	Currently, there are limited data to broadly assess the status of the US healthy infant gut microbiome.
33479326	0	34	theme	infant	28:33	arg1	insights					12:19	Metagenomic insights	0:19	Metagenomic insights of the infant	0:33	Metagenomic insights of the infant microbiome community structure and function across multiple sites in the United States.
33479326	4	35	theme	HMOs	948:951	arg1	utilization					953:963	HMOs utilization	948:963	HMOs utilization	948:963	Bifidobacterium were less abundant than generally expected and the species identified, including B. breve, B. longum and B. bifidum, had limited genetic capacity to metabolize human milk oligosaccharides (HMOs), while B. infantis strains with a complete capacity for HMOs utilization were found to be exceptionally rare.
33479326	5	36	theme	gut	1169:1171	arg1	microbiome					1173:1182	the contemporary US infant gut microbiome	1142:1182	the contemporary US infant gut microbiome	1142:1182	Considering microbiome composition and functional capacity, this survey revealed a previously unappreciated dysbiosis that is widespread in the contemporary US infant gut microbiome.
33479326	1	37	theme	infant	296:301	arg1	host					303:306	the infant host	292:306	the infant host	292:306	The gut microbiome plays an important role in early life, protecting newborns from enteric pathogens, promoting immune system development and providing key functions to the infant host.
33479326	3	38	theme	signatures	616:625	arg1	levels					495:500	high levels	490:500	high levels	490:500	To address this gap, we performed a multi-state metagenomic survey and found high levels of bacteria associated with enteric inflammation (e.g. Escherichia, Klebsiella), antibiotic resistance genes, and signatures of dysbiosis, independent of location, age, and diet.
33479326	2	39	dep	microbiome	401:410	arg1	US					379:380	US	379:380	US	379:380	Currently, there are limited data to broadly assess the status of the US healthy infant gut microbiome.
33479326	3	40	theme	metagenomic	461:471	arg1	survey					473:478	a multi-state metagenomic survey	447:478	a multi-state metagenomic survey	447:478	To address this gap, we performed a multi-state metagenomic survey and found high levels of bacteria associated with enteric inflammation (e.g. Escherichia, Klebsiella), antibiotic resistance genes, and signatures of dysbiosis, independent of location, age, and diet.
33479326	0	41	theme	community	46:54	arg1	structure					56:64	community structure	46:64	community structure	46:64	Metagenomic insights of the infant microbiome community structure and function across multiple sites in the United States.
33479326	3	42	dep	bacteria	505:512	arg1	Klebsiella					570:579	Klebsiella	570:579	Klebsiella	570:579	To address this gap, we performed a multi-state metagenomic survey and found high levels of bacteria associated with enteric inflammation (e.g. Escherichia, Klebsiella), antibiotic resistance genes, and signatures of dysbiosis, independent of location, age, and diet.
33479326	3	42	dep	bacteria	505:512	arg1	Escherichia					557:567	e.g. Escherichia	552:567	e.g. Escherichia	552:567	To address this gap, we performed a multi-state metagenomic survey and found high levels of bacteria associated with enteric inflammation (e.g. Escherichia, Klebsiella), antibiotic resistance genes, and signatures of dysbiosis, independent of location, age, and diet.
33479326	4	43	theme	milk	863:866	arg1	HMOs					886:889	HMOs	886:889	HMOs	886:889	Bifidobacterium were less abundant than generally expected and the species identified, including B. breve, B. longum and B. bifidum, had limited genetic capacity to metabolize human milk oligosaccharides (HMOs), while B. infantis strains with a complete capacity for HMOs utilization were found to be exceptionally rare.
33479326	4	43	theme	milk	863:866	arg1	oligosaccharides					868:883	human milk oligosaccharides	857:883	human milk oligosaccharides (HMOs)	857:890	Bifidobacterium were less abundant than generally expected and the species identified, including B. breve, B. longum and B. bifidum, had limited genetic capacity to metabolize human milk oligosaccharides (HMOs), while B. infantis strains with a complete capacity for HMOs utilization were found to be exceptionally rare.
33479326	3	44	theme	location	656:663	arg1	independent					641:651	independent	641:651	independent	641:651	To address this gap, we performed a multi-state metagenomic survey and found high levels of bacteria associated with enteric inflammation (e.g. Escherichia, Klebsiella), antibiotic resistance genes, and signatures of dysbiosis, independent of location, age, and diet.
33479326	3	45	theme	genes	605:609	arg1	levels					495:500	high levels	490:500	high levels	490:500	To address this gap, we performed a multi-state metagenomic survey and found high levels of bacteria associated with enteric inflammation (e.g. Escherichia, Klebsiella), antibiotic resistance genes, and signatures of dysbiosis, independent of location, age, and diet.
33479326	4	46	with	strains	911:917	arg1	capacity					935:942	a complete capacity	924:942	a complete capacity for HMOs utilization	924:963	Bifidobacterium were less abundant than generally expected and the species identified, including B. breve, B. longum and B. bifidum, had limited genetic capacity to metabolize human milk oligosaccharides (HMOs), while B. infantis strains with a complete capacity for HMOs utilization were found to be exceptionally rare.
33479326	1	47	theme	enteric	206:212	arg1	pathogens					214:222	enteric pathogens	206:222	enteric pathogens	206:222	The gut microbiome plays an important role in early life, protecting newborns from enteric pathogens, promoting immune system development and providing key functions to the infant host.
33479326	1	48	from	pathogens	214:222	arg1	newborns					192:199	newborns	192:199	newborns from enteric pathogens	192:222	The gut microbiome plays an important role in early life, protecting newborns from enteric pathogens, promoting immune system development and providing key functions to the infant host.
33479326	1	49	theme	gut	127:129	arg1	microbiome					131:140	The gut microbiome	123:140	The gut microbiome	123:140	The gut microbiome plays an important role in early life, protecting newborns from enteric pathogens, promoting immune system development and providing key functions to the infant host.
33479326	0	50	from	sites	95:99	arg1	States					115:120	the United States	104:120	the United States	104:120	Metagenomic insights of the infant microbiome community structure and function across multiple sites in the United States.
33479326	3	51	theme	antibiotic	583:592	arg1	genes					605:609	antibiotic resistance genes	583:609	antibiotic resistance genes	583:609	To address this gap, we performed a multi-state metagenomic survey and found high levels of bacteria associated with enteric inflammation (e.g. Escherichia, Klebsiella), antibiotic resistance genes, and signatures of dysbiosis, independent of location, age, and diet.
33479326	3	52	theme	high	490:493	arg1	levels					495:500	high levels	490:500	high levels	490:500	To address this gap, we performed a multi-state metagenomic survey and found high levels of bacteria associated with enteric inflammation (e.g. Escherichia, Klebsiella), antibiotic resistance genes, and signatures of dysbiosis, independent of location, age, and diet.
33479326	5	53	dep	contemporary	1146:1157	arg1	US					1159:1160	US	1159:1160	US	1159:1160	Considering microbiome composition and functional capacity, this survey revealed a previously unappreciated dysbiosis that is widespread in the contemporary US infant gut microbiome.
33479326	3	54	theme	resistance	594:603	arg1	genes					605:609	antibiotic resistance genes	583:609	antibiotic resistance genes	583:609	To address this gap, we performed a multi-state metagenomic survey and found high levels of bacteria associated with enteric inflammation (e.g. Escherichia, Klebsiella), antibiotic resistance genes, and signatures of dysbiosis, independent of location, age, and diet.
34130600	0	0	theme	defective	89:97	arg1	autophagy					99:107	defective autophagy	89:107	defective autophagy	89:107	The spectrum of neurodevelopmental, neuromuscular and neurodegenerative disorders due to defective autophagy.
34130600	7	1	theme	ciliopathies	1364:1375	arg1	defects					1418:1424	ciliopathies, glycosylation and vesicular trafficking defects	1364:1424	ciliopathies, glycosylation and vesicular trafficking defects	1364:1424	The differential diagnosis comprises a wide range of other multisystem disorders, including mitochondrial, glycogen and lysosomal storage disorders, as well as ciliopathies, glycosylation and vesicular trafficking defects.
34130600	7	2	theme	disorders	1275:1283	arg1	range					1248:1252	a wide range	1241:1252	a wide range of other multisystem disorders, including mitochondrial, glycogen and lysosomal storage disorders, as well as ciliopathies, glycosylation and vesicular trafficking defects	1241:1424	The differential diagnosis comprises a wide range of other multisystem disorders, including mitochondrial, glycogen and lysosomal storage disorders, as well as ciliopathies, glycosylation and vesicular trafficking defects.
34130600	7	2	theme	disorders	1275:1283	arg1	defects					1418:1424	ciliopathies, glycosylation and vesicular trafficking defects	1364:1424	ciliopathies, glycosylation and vesicular trafficking defects	1364:1424	The differential diagnosis comprises a wide range of other multisystem disorders, including mitochondrial, glycogen and lysosomal storage disorders, as well as ciliopathies, glycosylation and vesicular trafficking defects.
34130600	7	2	theme	disorders	1275:1283	arg1	disorders					1342:1350	mitochondrial, glycogen and lysosomal storage disorders	1296:1350	mitochondrial, glycogen and lysosomal storage disorders	1296:1350	The differential diagnosis comprises a wide range of other multisystem disorders, including mitochondrial, glycogen and lysosomal storage disorders, as well as ciliopathies, glycosylation and vesicular trafficking defects.
34130600	4	3	theme	prominent	781:789	arg1	features					804:811	prominent neurological features	781:811	prominent neurological features	781:811	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	3	theme	prominent	781:789	arg1	abnormalities					696:708	Structural CNS abnormalities	681:708	Structural CNS abnormalities	681:708	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	3	theme	prominent	781:789	arg1	involvement					722:732	cerebellar involvement	711:732	cerebellar involvement	711:732	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	3	theme	prominent	781:789	arg1	pathology					767:775	peripheral nerve pathology	750:775	peripheral nerve pathology	750:775	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	3	theme	prominent	781:789	arg1	spasticity					735:744	spasticity	735:744	spasticity	735:744	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	6	4	theme	associated	1138:1147	arg1	myopathy					1149:1156	an associated myopathy	1135:1156	an associated myopathy	1135:1156	Additionally, an associated myopathy has been characterized in several conditions.
34130600	8	5	theme	autophagy	1484:1492	arg1	disorders					1471:1479	the congenital disorders	1456:1479	the congenital disorders of autophagy	1456:1492	The clinical overlap between the congenital disorders of autophagy and these conditions reflects the multiple roles of the proteins and/or emerging molecular connections between the pathways implicated and suggests an exciting area for future research.
34130600	7	6	theme	multisystem	1263:1273	arg1	defects					1418:1424	ciliopathies, glycosylation and vesicular trafficking defects	1364:1424	ciliopathies, glycosylation and vesicular trafficking defects	1364:1424	The differential diagnosis comprises a wide range of other multisystem disorders, including mitochondrial, glycogen and lysosomal storage disorders, as well as ciliopathies, glycosylation and vesicular trafficking defects.
34130600	7	6	theme	multisystem	1263:1273	arg1	disorders					1275:1283	other multisystem disorders	1257:1283	other multisystem disorders	1257:1283	The differential diagnosis comprises a wide range of other multisystem disorders, including mitochondrial, glycogen and lysosomal storage disorders, as well as ciliopathies, glycosylation and vesicular trafficking defects.
34130600	7	6	theme	multisystem	1263:1273	arg1	disorders					1342:1350	mitochondrial, glycogen and lysosomal storage disorders	1296:1350	mitochondrial, glycogen and lysosomal storage disorders	1296:1350	The differential diagnosis comprises a wide range of other multisystem disorders, including mitochondrial, glycogen and lysosomal storage disorders, as well as ciliopathies, glycosylation and vesicular trafficking defects.
34130600	5	7	theme	disorder	1029:1036	arg1	background					994:1003	the background	990:1003	the background of a neurodevelopmental disorder	990:1036	A typically biphasic disease course of late-onset neurodegeneration occurring on the background of a neurodevelopmental disorder further supports a role of autophagy in both neuronal development and maintenance.
34130600	1	8	theme	core	178:181	arg1	components					183:192	core components	178:192	core components of the autophagy machinery	178:219	Primary dysfunction of autophagy due to Mendelian defects affecting core components of the autophagy machinery or closely related proteins have recently emerged as an important cause of genetic disease.
34130600	5	9	theme	neurodevelopmental	1010:1027	arg1	disorder					1029:1036	a neurodevelopmental disorder	1008:1036	a neurodevelopmental disorder	1008:1036	A typically biphasic disease course of late-onset neurodegeneration occurring on the background of a neurodevelopmental disorder further supports a role of autophagy in both neuronal development and maintenance.
34130600	8	10	theme	proteins	1550:1557	arg1	roles					1537:1541	the multiple roles	1524:1541	the multiple roles of the proteins	1524:1557	The clinical overlap between the congenital disorders of autophagy and these conditions reflects the multiple roles of the proteins and/or emerging molecular connections between the pathways implicated and suggests an exciting area for future research.
34130600	4	11	theme	autophagic	885:894	arg1	disturbance					896:906	autophagic disturbance	885:906	autophagic disturbance	885:906	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	7	12	theme	other	1257:1261	arg1	defects					1418:1424	ciliopathies, glycosylation and vesicular trafficking defects	1364:1424	ciliopathies, glycosylation and vesicular trafficking defects	1364:1424	The differential diagnosis comprises a wide range of other multisystem disorders, including mitochondrial, glycogen and lysosomal storage disorders, as well as ciliopathies, glycosylation and vesicular trafficking defects.
34130600	7	12	theme	other	1257:1261	arg1	disorders					1275:1283	other multisystem disorders	1257:1283	other multisystem disorders	1257:1283	The differential diagnosis comprises a wide range of other multisystem disorders, including mitochondrial, glycogen and lysosomal storage disorders, as well as ciliopathies, glycosylation and vesicular trafficking defects.
34130600	7	12	theme	other	1257:1261	arg1	disorders					1342:1350	mitochondrial, glycogen and lysosomal storage disorders	1296:1350	mitochondrial, glycogen and lysosomal storage disorders	1296:1350	The differential diagnosis comprises a wide range of other multisystem disorders, including mitochondrial, glycogen and lysosomal storage disorders, as well as ciliopathies, glycosylation and vesicular trafficking defects.
34130600	4	13	theme	nerve	761:765	arg1	features					804:811	prominent neurological features	781:811	prominent neurological features	781:811	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	13	theme	nerve	761:765	arg1	abnormalities					696:708	Structural CNS abnormalities	681:708	Structural CNS abnormalities	681:708	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	13	theme	nerve	761:765	arg1	involvement					722:732	cerebellar involvement	711:732	cerebellar involvement	711:732	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	13	theme	nerve	761:765	arg1	pathology					767:775	peripheral nerve pathology	750:775	peripheral nerve pathology	750:775	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	13	theme	nerve	761:765	arg1	spasticity					735:744	spasticity	735:744	spasticity	735:744	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	14	theme	neuronal	861:868	arg1	populations					870:880	certain neuronal populations	853:880	certain neuronal populations	853:880	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	5	15	theme	neuronal	1083:1090	arg1	development					1092:1102	neuronal development	1083:1102	neuronal development	1083:1102	A typically biphasic disease course of late-onset neurodegeneration occurring on the background of a neurodevelopmental disorder further supports a role of autophagy in both neuronal development and maintenance.
34130600	1	16	theme	important	277:285	arg1	cause					287:291	an important cause	274:291	an important cause of genetic disease	274:310	Primary dysfunction of autophagy due to Mendelian defects affecting core components of the autophagy machinery or closely related proteins have recently emerged as an important cause of genetic disease.
34130600	5	17	theme	autophagy	1065:1073	arg1	role					1057:1060	a role	1055:1060	a role of autophagy in both neuronal development and maintenance	1055:1118	A typically biphasic disease course of late-onset neurodegeneration occurring on the background of a neurodevelopmental disorder further supports a role of autophagy in both neuronal development and maintenance.
34130600	9	18	theme	congenital	1704:1713	arg1	disorders					1715:1723	congenital disorders	1704:1723	congenital disorders of autophagy	1704:1736	Therapy development for congenital disorders of autophagy is still in its infancy but may result in the identification of molecules that target autophagy more specifically than currently available compounds.
34130600	3	19	theme	variable	596:603	arg1	combinations					605:616	variable combinations	596:616	variable combinations of neurological, neuromuscular and multisystem manifestations	596:678	Early-onset (or congenital) disorders of autophagy often share a recognizable "clinical signature," including variable combinations of neurological, neuromuscular and multisystem manifestations.
34130600	7	20	theme	trafficking	1406:1416	arg1	defects					1418:1424	ciliopathies, glycosylation and vesicular trafficking defects	1364:1424	ciliopathies, glycosylation and vesicular trafficking defects	1364:1424	The differential diagnosis comprises a wide range of other multisystem disorders, including mitochondrial, glycogen and lysosomal storage disorders, as well as ciliopathies, glycosylation and vesicular trafficking defects.
34130600	4	21	theme	certain	853:859	arg1	populations					870:880	certain neuronal populations	853:880	certain neuronal populations	853:880	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	3	22	theme	autophagy	527:535	arg1	disorders					514:522	Early-onset (or congenital) disorders	486:522	Early-onset (or congenital) disorders of autophagy	486:535	Early-onset (or congenital) disorders of autophagy often share a recognizable "clinical signature," including variable combinations of neurological, neuromuscular and multisystem manifestations.
34130600	9	23	theme	Therapy	1680:1686	arg1	development					1688:1698	Therapy development	1680:1698	Therapy development for congenital disorders of autophagy	1680:1736	Therapy development for congenital disorders of autophagy is still in its infancy but may result in the identification of molecules that target autophagy more specifically than currently available compounds.
34130600	5	24	theme	late-onset	948:957	arg1	neurodegeneration					959:975	late-onset neurodegeneration	948:975	late-onset neurodegeneration	948:975	A typically biphasic disease course of late-onset neurodegeneration occurring on the background of a neurodevelopmental disorder further supports a role of autophagy in both neuronal development and maintenance.
34130600	3	25	theme	Early-onset	486:496	arg1	disorders					514:522	Early-onset (or congenital) disorders	486:522	Early-onset (or congenital) disorders of autophagy	486:535	Early-onset (or congenital) disorders of autophagy often share a recognizable "clinical signature," including variable combinations of neurological, neuromuscular and multisystem manifestations.
34130600	3	26	dep	manifestations	665:678	arg1	multisystem					653:663	multisystem	653:663	multisystem	653:663	Early-onset (or congenital) disorders of autophagy often share a recognizable "clinical signature," including variable combinations of neurological, neuromuscular and multisystem manifestations.
34130600	3	26	dep	manifestations	665:678	arg1	neuromuscular					635:647	neuromuscular	635:647	neuromuscular	635:647	Early-onset (or congenital) disorders of autophagy often share a recognizable "clinical signature," including variable combinations of neurological, neuromuscular and multisystem manifestations.
34130600	3	26	dep	manifestations	665:678	arg1	manifestations					665:678	neurological, neuromuscular and multisystem manifestations	621:678	manifestations	665:678	Early-onset (or congenital) disorders of autophagy often share a recognizable "clinical signature," including variable combinations of neurological, neuromuscular and multisystem manifestations.
34130600	5	27	from	role	1057:1060	arg1	maintenance					1108:1118	maintenance	1108:1118	maintenance	1108:1118	A typically biphasic disease course of late-onset neurodegeneration occurring on the background of a neurodevelopmental disorder further supports a role of autophagy in both neuronal development and maintenance.
34130600	5	27	from	role	1057:1060	arg1	development					1092:1102	neuronal development	1083:1102	neuronal development	1083:1102	A typically biphasic disease course of late-onset neurodegeneration occurring on the background of a neurodevelopmental disorder further supports a role of autophagy in both neuronal development and maintenance.
34130600	4	28	theme	populations	870:880	arg1	vulnerability					836:848	a specific vulnerability	825:848	a specific vulnerability of certain neuronal populations to autophagic disturbance	825:906	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	2	29	theme	neurodevelopmental	410:427	arg1	disorders					475:483	severe early-onset neurodevelopmental and more common adult-onset neurodegenerative disorders	391:483	severe early-onset neurodevelopmental and more common adult-onset neurodegenerative disorders	391:483	This novel group of human disorders may present throughout life and comprises severe early-onset neurodevelopmental and more common adult-onset neurodegenerative disorders.
34130600	7	30	theme	storage	1334:1340	arg1	disorders					1342:1350	mitochondrial, glycogen and lysosomal storage disorders	1296:1350	mitochondrial, glycogen and lysosomal storage disorders	1296:1350	The differential diagnosis comprises a wide range of other multisystem disorders, including mitochondrial, glycogen and lysosomal storage disorders, as well as ciliopathies, glycosylation and vesicular trafficking defects.
34130600	9	31	theme	autophagy	1728:1736	arg1	disorders					1715:1723	congenital disorders	1704:1723	congenital disorders of autophagy	1704:1736	Therapy development for congenital disorders of autophagy is still in its infancy but may result in the identification of molecules that target autophagy more specifically than currently available compounds.
34130600	4	32	theme	specific	827:834	arg1	vulnerability					836:848	a specific vulnerability	825:848	a specific vulnerability of certain neuronal populations to autophagic disturbance	825:906	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	33	theme	peripheral	750:759	arg1	features					804:811	prominent neurological features	781:811	prominent neurological features	781:811	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	33	theme	peripheral	750:759	arg1	abnormalities					696:708	Structural CNS abnormalities	681:708	Structural CNS abnormalities	681:708	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	33	theme	peripheral	750:759	arg1	involvement					722:732	cerebellar involvement	711:732	cerebellar involvement	711:732	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	33	theme	peripheral	750:759	arg1	pathology					767:775	peripheral nerve pathology	750:775	peripheral nerve pathology	750:775	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	33	theme	peripheral	750:759	arg1	spasticity					735:744	spasticity	735:744	spasticity	735:744	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	5	34	theme	biphasic	921:928	arg1	course					938:943	A typically biphasic disease course	909:943	A typically biphasic disease course of late-onset neurodegeneration occurring on the background of a neurodevelopmental disorder	909:1036	A typically biphasic disease course of late-onset neurodegeneration occurring on the background of a neurodevelopmental disorder further supports a role of autophagy in both neuronal development and maintenance.
34130600	7	35	theme	wide	1243:1246	arg1	range					1248:1252	a wide range	1241:1252	a wide range of other multisystem disorders, including mitochondrial, glycogen and lysosomal storage disorders, as well as ciliopathies, glycosylation and vesicular trafficking defects	1241:1424	The differential diagnosis comprises a wide range of other multisystem disorders, including mitochondrial, glycogen and lysosomal storage disorders, as well as ciliopathies, glycosylation and vesicular trafficking defects.
34130600	7	35	theme	wide	1243:1246	arg1	defects					1418:1424	ciliopathies, glycosylation and vesicular trafficking defects	1364:1424	ciliopathies, glycosylation and vesicular trafficking defects	1364:1424	The differential diagnosis comprises a wide range of other multisystem disorders, including mitochondrial, glycogen and lysosomal storage disorders, as well as ciliopathies, glycosylation and vesicular trafficking defects.
34130600	7	35	theme	wide	1243:1246	arg1	disorders					1342:1350	mitochondrial, glycogen and lysosomal storage disorders	1296:1350	mitochondrial, glycogen and lysosomal storage disorders	1296:1350	The differential diagnosis comprises a wide range of other multisystem disorders, including mitochondrial, glycogen and lysosomal storage disorders, as well as ciliopathies, glycosylation and vesicular trafficking defects.
34130600	6	36	theme	several	1184:1190	arg1	conditions					1192:1201	several conditions	1184:1201	several conditions	1184:1201	Additionally, an associated myopathy has been characterized in several conditions.
34130600	3	37	theme	neurological	621:632	arg1	multisystem					653:663	multisystem	653:663	multisystem	653:663	Early-onset (or congenital) disorders of autophagy often share a recognizable "clinical signature," including variable combinations of neurological, neuromuscular and multisystem manifestations.
34130600	3	37	theme	neurological	621:632	arg1	neuromuscular					635:647	neuromuscular	635:647	neuromuscular	635:647	Early-onset (or congenital) disorders of autophagy often share a recognizable "clinical signature," including variable combinations of neurological, neuromuscular and multisystem manifestations.
34130600	3	37	theme	neurological	621:632	arg1	manifestations					665:678	neurological, neuromuscular and multisystem manifestations	621:678	manifestations	665:678	Early-onset (or congenital) disorders of autophagy often share a recognizable "clinical signature," including variable combinations of neurological, neuromuscular and multisystem manifestations.
34130600	2	38	theme	severe	391:396	arg1	disorders					475:483	severe early-onset neurodevelopmental and more common adult-onset neurodegenerative disorders	391:483	severe early-onset neurodevelopmental and more common adult-onset neurodegenerative disorders	391:483	This novel group of human disorders may present throughout life and comprises severe early-onset neurodevelopmental and more common adult-onset neurodegenerative disorders.
34130600	0	39	theme	neurodevelopmental	16:33	arg1	disorders					72:80	neurodevelopmental, neuromuscular and neurodegenerative disorders	16:80	neurodevelopmental, neuromuscular and neurodegenerative disorders	16:80	The spectrum of neurodevelopmental, neuromuscular and neurodegenerative disorders due to defective autophagy.
34130600	1	40	theme	genetic	296:302	arg1	disease					304:310	genetic disease	296:310	genetic disease	296:310	Primary dysfunction of autophagy due to Mendelian defects affecting core components of the autophagy machinery or closely related proteins have recently emerged as an important cause of genetic disease.
34130600	1	41	theme	Primary	110:116	arg1	dysfunction					118:128	Primary dysfunction	110:128	Primary dysfunction of autophagy due to Mendelian defects affecting core components of the autophagy machinery or closely related proteins	110:247	Primary dysfunction of autophagy due to Mendelian defects affecting core components of the autophagy machinery or closely related proteins have recently emerged as an important cause of genetic disease.
34130600	8	42	theme	molecular	1575:1583	arg1	connections					1585:1595	emerging molecular connections	1566:1595	emerging molecular connections between the pathways implicated	1566:1627	The clinical overlap between the congenital disorders of autophagy and these conditions reflects the multiple roles of the proteins and/or emerging molecular connections between the pathways implicated and suggests an exciting area for future research.
34130600	2	43	theme	neurodegenerative	457:473	arg1	disorders					475:483	severe early-onset neurodevelopmental and more common adult-onset neurodegenerative disorders	391:483	severe early-onset neurodevelopmental and more common adult-onset neurodegenerative disorders	391:483	This novel group of human disorders may present throughout life and comprises severe early-onset neurodevelopmental and more common adult-onset neurodegenerative disorders.
34130600	7	44	theme	lysosomal	1324:1332	arg1	disorders					1342:1350	mitochondrial, glycogen and lysosomal storage disorders	1296:1350	mitochondrial, glycogen and lysosomal storage disorders	1296:1350	The differential diagnosis comprises a wide range of other multisystem disorders, including mitochondrial, glycogen and lysosomal storage disorders, as well as ciliopathies, glycosylation and vesicular trafficking defects.
34130600	1	45	theme	disease	304:310	arg1	cause					287:291	an important cause	274:291	an important cause of genetic disease	274:310	Primary dysfunction of autophagy due to Mendelian defects affecting core components of the autophagy machinery or closely related proteins have recently emerged as an important cause of genetic disease.
34130600	8	46	theme	emerging	1566:1573	arg1	connections					1585:1595	emerging molecular connections	1566:1595	emerging molecular connections between the pathways implicated	1566:1627	The clinical overlap between the congenital disorders of autophagy and these conditions reflects the multiple roles of the proteins and/or emerging molecular connections between the pathways implicated and suggests an exciting area for future research.
34130600	3	47	theme	recognizable	551:562	arg1	signature					574:582	a recognizable "clinical signature	549:582	a recognizable "clinical signature	549:582	Early-onset (or congenital) disorders of autophagy often share a recognizable "clinical signature," including variable combinations of neurological, neuromuscular and multisystem manifestations.
34130600	2	48	theme	adult-onset	445:455	arg1	disorders					475:483	severe early-onset neurodevelopmental and more common adult-onset neurodegenerative disorders	391:483	severe early-onset neurodevelopmental and more common adult-onset neurodegenerative disorders	391:483	This novel group of human disorders may present throughout life and comprises severe early-onset neurodevelopmental and more common adult-onset neurodegenerative disorders.
34130600	0	49	theme	neuromuscular	36:48	arg1	disorders					72:80	neurodevelopmental, neuromuscular and neurodegenerative disorders	16:80	neurodevelopmental, neuromuscular and neurodegenerative disorders	16:80	The spectrum of neurodevelopmental, neuromuscular and neurodegenerative disorders due to defective autophagy.
34130600	4	50	theme	neurological	791:802	arg1	features					804:811	prominent neurological features	781:811	prominent neurological features	781:811	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	50	theme	neurological	791:802	arg1	abnormalities					696:708	Structural CNS abnormalities	681:708	Structural CNS abnormalities	681:708	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	50	theme	neurological	791:802	arg1	involvement					722:732	cerebellar involvement	711:732	cerebellar involvement	711:732	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	50	theme	neurological	791:802	arg1	pathology					767:775	peripheral nerve pathology	750:775	peripheral nerve pathology	750:775	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	50	theme	neurological	791:802	arg1	spasticity					735:744	spasticity	735:744	spasticity	735:744	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	5	51	theme	neurodegeneration	959:975	arg1	course					938:943	A typically biphasic disease course	909:943	A typically biphasic disease course of late-onset neurodegeneration occurring on the background of a neurodevelopmental disorder	909:1036	A typically biphasic disease course of late-onset neurodegeneration occurring on the background of a neurodevelopmental disorder further supports a role of autophagy in both neuronal development and maintenance.
34130600	4	52	theme	cerebellar	711:720	arg1	features					804:811	prominent neurological features	781:811	prominent neurological features	781:811	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	52	theme	cerebellar	711:720	arg1	abnormalities					696:708	Structural CNS abnormalities	681:708	Structural CNS abnormalities	681:708	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	52	theme	cerebellar	711:720	arg1	involvement					722:732	cerebellar involvement	711:732	cerebellar involvement	711:732	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	52	theme	cerebellar	711:720	arg1	pathology					767:775	peripheral nerve pathology	750:775	peripheral nerve pathology	750:775	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	52	theme	cerebellar	711:720	arg1	spasticity					735:744	spasticity	735:744	spasticity	735:744	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	1	53	theme	autophagy	201:209	arg1	machinery					211:219	the autophagy machinery	197:219	the autophagy machinery	197:219	Primary dysfunction of autophagy due to Mendelian defects affecting core components of the autophagy machinery or closely related proteins have recently emerged as an important cause of genetic disease.
34130600	2	54	theme	common	438:443	arg1	disorders					475:483	severe early-onset neurodevelopmental and more common adult-onset neurodegenerative disorders	391:483	severe early-onset neurodevelopmental and more common adult-onset neurodegenerative disorders	391:483	This novel group of human disorders may present throughout life and comprises severe early-onset neurodevelopmental and more common adult-onset neurodegenerative disorders.
34130600	7	55	theme	glycogen	1311:1318	arg1	disorders					1342:1350	mitochondrial, glycogen and lysosomal storage disorders	1296:1350	mitochondrial, glycogen and lysosomal storage disorders	1296:1350	The differential diagnosis comprises a wide range of other multisystem disorders, including mitochondrial, glycogen and lysosomal storage disorders, as well as ciliopathies, glycosylation and vesicular trafficking defects.
34130600	7	56	theme	differential	1208:1219	arg1	diagnosis					1221:1229	The differential diagnosis	1204:1229	The differential diagnosis	1204:1229	The differential diagnosis comprises a wide range of other multisystem disorders, including mitochondrial, glycogen and lysosomal storage disorders, as well as ciliopathies, glycosylation and vesicular trafficking defects.
34130600	5	57	theme	disease	930:936	arg1	course					938:943	A typically biphasic disease course	909:943	A typically biphasic disease course of late-onset neurodegeneration occurring on the background of a neurodevelopmental disorder	909:1036	A typically biphasic disease course of late-onset neurodegeneration occurring on the background of a neurodevelopmental disorder further supports a role of autophagy in both neuronal development and maintenance.
34130600	1	58	theme	related	232:238	arg1	proteins					240:247	closely related proteins	224:247	closely related proteins	224:247	Primary dysfunction of autophagy due to Mendelian defects affecting core components of the autophagy machinery or closely related proteins have recently emerged as an important cause of genetic disease.
34130600	0	59	theme	neurodegenerative	54:70	arg1	disorders					72:80	neurodevelopmental, neuromuscular and neurodegenerative disorders	16:80	neurodevelopmental, neuromuscular and neurodegenerative disorders	16:80	The spectrum of neurodevelopmental, neuromuscular and neurodegenerative disorders due to defective autophagy.
34130600	8	60	theme	exciting	1645:1652	arg1	area					1654:1657	an exciting area	1642:1657	an exciting area for future research	1642:1677	The clinical overlap between the congenital disorders of autophagy and these conditions reflects the multiple roles of the proteins and/or emerging molecular connections between the pathways implicated and suggests an exciting area for future research.
34130600	8	61	theme	congenital	1460:1469	arg1	disorders					1471:1479	the congenital disorders	1456:1479	the congenital disorders of autophagy	1456:1492	The clinical overlap between the congenital disorders of autophagy and these conditions reflects the multiple roles of the proteins and/or emerging molecular connections between the pathways implicated and suggests an exciting area for future research.
34130600	7	62	theme	mitochondrial	1296:1308	arg1	disorders					1342:1350	mitochondrial, glycogen and lysosomal storage disorders	1296:1350	mitochondrial, glycogen and lysosomal storage disorders	1296:1350	The differential diagnosis comprises a wide range of other multisystem disorders, including mitochondrial, glycogen and lysosomal storage disorders, as well as ciliopathies, glycosylation and vesicular trafficking defects.
34130600	8	63	theme	future	1663:1668	arg1	research					1670:1677	future research	1663:1677	future research	1663:1677	The clinical overlap between the congenital disorders of autophagy and these conditions reflects the multiple roles of the proteins and/or emerging molecular connections between the pathways implicated and suggests an exciting area for future research.
34130600	9	64	theme	molecules	1802:1810	arg1	identification					1784:1797	the identification	1780:1797	the identification of molecules that target autophagy more specifically than currently available compounds	1780:1885	Therapy development for congenital disorders of autophagy is still in its infancy but may result in the identification of molecules that target autophagy more specifically than currently available compounds.
34130600	9	65	theme	available	1867:1875	arg1	compounds					1877:1885	currently available compounds	1857:1885	currently available compounds	1857:1885	Therapy development for congenital disorders of autophagy is still in its infancy but may result in the identification of molecules that target autophagy more specifically than currently available compounds.
34130600	1	66	theme	autophagy	133:141	arg1	dysfunction					118:128	Primary dysfunction	110:128	Primary dysfunction of autophagy due to Mendelian defects affecting core components of the autophagy machinery or closely related proteins	110:247	Primary dysfunction of autophagy due to Mendelian defects affecting core components of the autophagy machinery or closely related proteins have recently emerged as an important cause of genetic disease.
34130600	3	67	theme	manifestations	665:678	arg1	combinations					605:616	variable combinations	596:616	variable combinations of neurological, neuromuscular and multisystem manifestations	596:678	Early-onset (or congenital) disorders of autophagy often share a recognizable "clinical signature," including variable combinations of neurological, neuromuscular and multisystem manifestations.
34130600	2	68	theme	disorders	339:347	arg1	group					324:328	This novel group	313:328	This novel group of human disorders	313:347	This novel group of human disorders may present throughout life and comprises severe early-onset neurodevelopmental and more common adult-onset neurodegenerative disorders.
34130600	2	68	theme	disorders	339:347	arg1	disorders					339:347	human disorders	333:347	human disorders	333:347	This novel group of human disorders may present throughout life and comprises severe early-onset neurodevelopmental and more common adult-onset neurodegenerative disorders.
34130600	2	69	theme	novel	318:322	arg1	group					324:328	This novel group	313:328	This novel group of human disorders	313:347	This novel group of human disorders may present throughout life and comprises severe early-onset neurodevelopmental and more common adult-onset neurodegenerative disorders.
34130600	2	69	theme	novel	318:322	arg1	disorders					339:347	human disorders	333:347	human disorders	333:347	This novel group of human disorders may present throughout life and comprises severe early-onset neurodevelopmental and more common adult-onset neurodegenerative disorders.
34130600	8	70	theme	multiple	1528:1535	arg1	roles					1537:1541	the multiple roles	1524:1541	the multiple roles of the proteins	1524:1557	The clinical overlap between the congenital disorders of autophagy and these conditions reflects the multiple roles of the proteins and/or emerging molecular connections between the pathways implicated and suggests an exciting area for future research.
34130600	2	71	dep	severe	391:396	arg1	early-onset					398:408	early-onset	398:408	early-onset	398:408	This novel group of human disorders may present throughout life and comprises severe early-onset neurodevelopmental and more common adult-onset neurodegenerative disorders.
34130600	2	72	theme	human	333:337	arg1	disorders					339:347	human disorders	333:347	human disorders	333:347	This novel group of human disorders may present throughout life and comprises severe early-onset neurodevelopmental and more common adult-onset neurodegenerative disorders.
34130600	7	73	theme	glycosylation	1378:1390	arg1	defects					1418:1424	ciliopathies, glycosylation and vesicular trafficking defects	1364:1424	ciliopathies, glycosylation and vesicular trafficking defects	1364:1424	The differential diagnosis comprises a wide range of other multisystem disorders, including mitochondrial, glycogen and lysosomal storage disorders, as well as ciliopathies, glycosylation and vesicular trafficking defects.
34130600	3	74	theme	congenital	502:511	arg1	disorders					514:522	Early-onset (or congenital) disorders	486:522	Early-onset (or congenital) disorders of autophagy	486:535	Early-onset (or congenital) disorders of autophagy often share a recognizable "clinical signature," including variable combinations of neurological, neuromuscular and multisystem manifestations.
34130600	0	75	dep	spectrum	4:11	arg1	autophagy					99:107	defective autophagy	89:107	defective autophagy	89:107	The spectrum of neurodevelopmental, neuromuscular and neurodegenerative disorders due to defective autophagy.
34130600	1	76	theme	machinery	211:219	arg1	components					183:192	core components	178:192	core components of the autophagy machinery	178:219	Primary dysfunction of autophagy due to Mendelian defects affecting core components of the autophagy machinery or closely related proteins have recently emerged as an important cause of genetic disease.
34130600	1	76	theme	machinery	211:219	arg1	proteins					240:247	closely related proteins	224:247	closely related proteins	224:247	Primary dysfunction of autophagy due to Mendelian defects affecting core components of the autophagy machinery or closely related proteins have recently emerged as an important cause of genetic disease.
34130600	1	77	theme	Mendelian	150:158	arg1	defects					160:166	Mendelian defects	150:166	Mendelian defects affecting core components of the autophagy machinery or closely related proteins	150:247	Primary dysfunction of autophagy due to Mendelian defects affecting core components of the autophagy machinery or closely related proteins have recently emerged as an important cause of genetic disease.
34130600	4	78	theme	CNS	692:694	arg1	features					804:811	prominent neurological features	781:811	prominent neurological features	781:811	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	78	theme	CNS	692:694	arg1	abnormalities					696:708	Structural CNS abnormalities	681:708	Structural CNS abnormalities	681:708	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	78	theme	CNS	692:694	arg1	involvement					722:732	cerebellar involvement	711:732	cerebellar involvement	711:732	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	78	theme	CNS	692:694	arg1	pathology					767:775	peripheral nerve pathology	750:775	peripheral nerve pathology	750:775	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	78	theme	CNS	692:694	arg1	spasticity					735:744	spasticity	735:744	spasticity	735:744	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	0	79	theme	disorders	72:80	arg1	spectrum					4:11	The spectrum	0:11	The spectrum of neurodevelopmental, neuromuscular and neurodegenerative disorders	0:80	The spectrum of neurodevelopmental, neuromuscular and neurodegenerative disorders due to defective autophagy.
34130600	3	80	theme	clinical	565:572	arg1	signature					574:582	a recognizable "clinical signature	549:582	a recognizable "clinical signature	549:582	Early-onset (or congenital) disorders of autophagy often share a recognizable "clinical signature," including variable combinations of neurological, neuromuscular and multisystem manifestations.
34130600	7	81	theme	vesicular	1396:1404	arg1	trafficking					1406:1416	vesicular trafficking	1396:1416	vesicular trafficking	1396:1416	The differential diagnosis comprises a wide range of other multisystem disorders, including mitochondrial, glycogen and lysosomal storage disorders, as well as ciliopathies, glycosylation and vesicular trafficking defects.
34130600	4	82	theme	Structural	681:690	arg1	features					804:811	prominent neurological features	781:811	prominent neurological features	781:811	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	82	theme	Structural	681:690	arg1	abnormalities					696:708	Structural CNS abnormalities	681:708	Structural CNS abnormalities	681:708	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	82	theme	Structural	681:690	arg1	involvement					722:732	cerebellar involvement	711:732	cerebellar involvement	711:732	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	82	theme	Structural	681:690	arg1	pathology					767:775	peripheral nerve pathology	750:775	peripheral nerve pathology	750:775	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34130600	4	82	theme	Structural	681:690	arg1	spasticity					735:744	spasticity	735:744	spasticity	735:744	Structural CNS abnormalities, cerebellar involvement, spasticity and peripheral nerve pathology are prominent neurological features, indicating a specific vulnerability of certain neuronal populations to autophagic disturbance.
34204022	4	0	theme	Robust	690:695	arg1	inflammation					709:720	Robust endothelial inflammation	690:720	Robust endothelial inflammation induced by LPS	690:735	Robust endothelial inflammation induced by LPS was linked to LDs composed of highly unsaturated lipids, as well as prostacyclin release.
34204022	2	1	theme	endothelial	365:375	arg1	inflammation					377:388	endothelial inflammation	365:388	endothelial inflammation in response to lipopolysaccharides (LPS)	365:429	Here, we adopted a multiparameter approach combining various imaging techniques at the nano- and microscale (Raman, AFM and fluorescence) to investigate endothelial inflammation in response to lipopolysaccharides (LPS) in vitro in human microvascular endothelial cells (HMEC-1) with a focus on lipid droplets (LDs) formation.
34204022	5	2	theme	cell	904:907	arg1	architecture					918:929	the cell membrane architecture	900:929	the cell membrane architecture	900:929	LPS-induced LDs were spatially associated with nanostructural changes in the cell membrane architecture.
34204022	6	3	theme	inflammation	1006:1017	arg1	component					981:989	an integral component	969:989	an integral component of endothelial inflammation induced by LPS	969:1032	In summary, LDs formation represents an integral component of endothelial inflammation induced by LPS.
34204022	3	4	theme	serum	683:687	arg1	presence					671:678	the presence	667:678	the presence of serum	667:687	Our results show that LPS-induced LDs in HMEC-1 have a composition depending on LPS-incubation time and their formation requires the presence of serum.
34204022	3	5	contain	have	586:589	arg1	LDs					572:574	LPS-induced LDs	560:574	LPS-induced LDs in HMEC-1	560:584	Our results show that LPS-induced LDs in HMEC-1 have a composition depending on LPS-incubation time and their formation requires the presence of serum.
34204022	3	5	contain	have	586:589	arg2	composition					593:603	a composition	591:603	a composition	591:603	Our results show that LPS-induced LDs in HMEC-1 have a composition depending on LPS-incubation time and their formation requires the presence of serum.
34204022	5	6	from	changes	889:895	arg1	architecture					918:929	the cell membrane architecture	900:929	the cell membrane architecture	900:929	LPS-induced LDs were spatially associated with nanostructural changes in the cell membrane architecture.
34204022	2	7	dep	microscale	309:318	arg1	Raman					321:325	Raman	321:325	Raman	321:325	Here, we adopted a multiparameter approach combining various imaging techniques at the nano- and microscale (Raman, AFM and fluorescence) to investigate endothelial inflammation in response to lipopolysaccharides (LPS) in vitro in human microvascular endothelial cells (HMEC-1) with a focus on lipid droplets (LDs) formation.
34204022	2	7	dep	microscale	309:318	arg1	fluorescence					336:347	fluorescence	336:347	fluorescence	336:347	Here, we adopted a multiparameter approach combining various imaging techniques at the nano- and microscale (Raman, AFM and fluorescence) to investigate endothelial inflammation in response to lipopolysaccharides (LPS) in vitro in human microvascular endothelial cells (HMEC-1) with a focus on lipid droplets (LDs) formation.
34204022	2	7	dep	microscale	309:318	arg1	AFM					328:330	AFM	328:330	AFM	328:330	Here, we adopted a multiparameter approach combining various imaging techniques at the nano- and microscale (Raman, AFM and fluorescence) to investigate endothelial inflammation in response to lipopolysaccharides (LPS) in vitro in human microvascular endothelial cells (HMEC-1) with a focus on lipid droplets (LDs) formation.
34204022	2	8	theme	droplets	512:519	arg1	formation					527:535	lipid droplets (LDs) formation	506:535	lipid droplets (LDs) formation	506:535	Here, we adopted a multiparameter approach combining various imaging techniques at the nano- and microscale (Raman, AFM and fluorescence) to investigate endothelial inflammation in response to lipopolysaccharides (LPS) in vitro in human microvascular endothelial cells (HMEC-1) with a focus on lipid droplets (LDs) formation.
34204022	2	9	theme	lipid	506:510	arg1	droplets					512:519	lipid droplets	506:519	lipid droplets (LDs) formation	506:535	Here, we adopted a multiparameter approach combining various imaging techniques at the nano- and microscale (Raman, AFM and fluorescence) to investigate endothelial inflammation in response to lipopolysaccharides (LPS) in vitro in human microvascular endothelial cells (HMEC-1) with a focus on lipid droplets (LDs) formation.
34204022	2	9	theme	lipid	506:510	arg1	LDs					522:524	LDs	522:524	LDs	522:524	Here, we adopted a multiparameter approach combining various imaging techniques at the nano- and microscale (Raman, AFM and fluorescence) to investigate endothelial inflammation in response to lipopolysaccharides (LPS) in vitro in human microvascular endothelial cells (HMEC-1) with a focus on lipid droplets (LDs) formation.
34204022	0	10	theme	Lipid	0:4	arg1	Droplets					6:13	Lipid Droplets	0:13	Lipid Droplets Formation	0:23	Lipid Droplets Formation Represents an Integral Component of Endothelial Inflammation Induced by LPS.
34204022	1	11	theme	cardiovascular	187:200	arg1	diseases					202:209	cardiovascular diseases	187:209	cardiovascular diseases	187:209	Endothelial inflammation is the hallmark of vascular pathology often proceeding with cardiovascular diseases.
34204022	0	12	theme	Droplets	6:13	arg1	Formation					15:23	Lipid Droplets Formation	0:23	Lipid Droplets Formation	0:23	Lipid Droplets Formation Represents an Integral Component of Endothelial Inflammation Induced by LPS.
34204022	2	13	theme	microvascular	449:461	arg1	HMEC-1					482:487	HMEC-1	482:487	HMEC-1	482:487	Here, we adopted a multiparameter approach combining various imaging techniques at the nano- and microscale (Raman, AFM and fluorescence) to investigate endothelial inflammation in response to lipopolysaccharides (LPS) in vitro in human microvascular endothelial cells (HMEC-1) with a focus on lipid droplets (LDs) formation.
34204022	2	13	theme	microvascular	449:461	arg1	cells					475:479	human microvascular endothelial cells	443:479	human microvascular endothelial cells (HMEC-1) with a focus on lipid droplets (LDs) formation	443:535	Here, we adopted a multiparameter approach combining various imaging techniques at the nano- and microscale (Raman, AFM and fluorescence) to investigate endothelial inflammation in response to lipopolysaccharides (LPS) in vitro in human microvascular endothelial cells (HMEC-1) with a focus on lipid droplets (LDs) formation.
34204022	1	14	dep	proceeding	171:180	arg1	hallmark					134:141	the hallmark	130:141	the hallmark of vascular pathology	130:163	Endothelial inflammation is the hallmark of vascular pathology often proceeding with cardiovascular diseases.
34204022	3	15	from	LDs	572:574	arg1	HMEC-1					579:584	HMEC-1	579:584	HMEC-1	579:584	Our results show that LPS-induced LDs in HMEC-1 have a composition depending on LPS-incubation time and their formation requires the presence of serum.
34204022	5	16	theme	nanostructural	874:887	arg1	changes					889:895	nanostructural changes	874:895	nanostructural changes in the cell membrane architecture	874:929	LPS-induced LDs were spatially associated with nanostructural changes in the cell membrane architecture.
34204022	2	17	theme	human	443:447	arg1	HMEC-1					482:487	HMEC-1	482:487	HMEC-1	482:487	Here, we adopted a multiparameter approach combining various imaging techniques at the nano- and microscale (Raman, AFM and fluorescence) to investigate endothelial inflammation in response to lipopolysaccharides (LPS) in vitro in human microvascular endothelial cells (HMEC-1) with a focus on lipid droplets (LDs) formation.
34204022	2	17	theme	human	443:447	arg1	cells					475:479	human microvascular endothelial cells	443:479	human microvascular endothelial cells (HMEC-1) with a focus on lipid droplets (LDs) formation	443:535	Here, we adopted a multiparameter approach combining various imaging techniques at the nano- and microscale (Raman, AFM and fluorescence) to investigate endothelial inflammation in response to lipopolysaccharides (LPS) in vitro in human microvascular endothelial cells (HMEC-1) with a focus on lipid droplets (LDs) formation.
34204022	6	18	theme	integral	972:979	arg1	component					981:989	an integral component	969:989	an integral component of endothelial inflammation induced by LPS	969:1032	In summary, LDs formation represents an integral component of endothelial inflammation induced by LPS.
34204022	2	19	from	inflammation	377:388	arg1	response					393:400	response	393:400	response to lipopolysaccharides (LPS)	393:429	Here, we adopted a multiparameter approach combining various imaging techniques at the nano- and microscale (Raman, AFM and fluorescence) to investigate endothelial inflammation in response to lipopolysaccharides (LPS) in vitro in human microvascular endothelial cells (HMEC-1) with a focus on lipid droplets (LDs) formation.
34204022	1	20	theme	Endothelial	102:112	arg1	inflammation					114:125	Endothelial inflammation	102:125	Endothelial inflammation	102:125	Endothelial inflammation is the hallmark of vascular pathology often proceeding with cardiovascular diseases.
34204022	3	21	theme	LPS-induced	560:570	arg1	LDs					572:574	LPS-induced LDs	560:574	LPS-induced LDs in HMEC-1	560:584	Our results show that LPS-induced LDs in HMEC-1 have a composition depending on LPS-incubation time and their formation requires the presence of serum.
34204022	5	22	theme	LPS-induced	827:837	arg1	LDs					839:841	LPS-induced LDs	827:841	LPS-induced LDs	827:841	LPS-induced LDs were spatially associated with nanostructural changes in the cell membrane architecture.
34204022	0	23	theme	Integral	39:46	arg1	Component					48:56	an Integral Component	36:56	an Integral Component of Endothelial Inflammation Induced by LPS	36:99	Lipid Droplets Formation Represents an Integral Component of Endothelial Inflammation Induced by LPS.
34204022	3	24	theme	LPS-incubation	618:631	arg1	time					633:636	LPS-incubation time	618:636	LPS-incubation time	618:636	Our results show that LPS-induced LDs in HMEC-1 have a composition depending on LPS-incubation time and their formation requires the presence of serum.
34204022	6	25	theme	LDs	944:946	arg1	formation					948:956	LDs formation	944:956	LDs formation	944:956	In summary, LDs formation represents an integral component of endothelial inflammation induced by LPS.
34204022	2	26	from	focus	497:501	arg1	formation					527:535	lipid droplets (LDs) formation	506:535	lipid droplets (LDs) formation	506:535	Here, we adopted a multiparameter approach combining various imaging techniques at the nano- and microscale (Raman, AFM and fluorescence) to investigate endothelial inflammation in response to lipopolysaccharides (LPS) in vitro in human microvascular endothelial cells (HMEC-1) with a focus on lipid droplets (LDs) formation.
34204022	2	27	theme	multiparameter	231:244	arg1	approach					246:253	a multiparameter approach	229:253	a multiparameter approach combining various imaging techniques at the nano- and microscale (Raman, AFM and fluorescence)	229:348	Here, we adopted a multiparameter approach combining various imaging techniques at the nano- and microscale (Raman, AFM and fluorescence) to investigate endothelial inflammation in response to lipopolysaccharides (LPS) in vitro in human microvascular endothelial cells (HMEC-1) with a focus on lipid droplets (LDs) formation.
34204022	5	28	theme	membrane	909:916	arg1	architecture					918:929	the cell membrane architecture	900:929	the cell membrane architecture	900:929	LPS-induced LDs were spatially associated with nanostructural changes in the cell membrane architecture.
34204022	4	29	attach	linked	741:746	arg2	inflammation					709:720	Robust endothelial inflammation	690:720	Robust endothelial inflammation induced by LPS	690:735	Robust endothelial inflammation induced by LPS was linked to LDs composed of highly unsaturated lipids, as well as prostacyclin release.
34204022	4	29	attach	linked	741:746	arg1	LDs					751:753	LDs	751:753	LDs composed of highly unsaturated lipids	751:791	Robust endothelial inflammation induced by LPS was linked to LDs composed of highly unsaturated lipids, as well as prostacyclin release.
34204022	4	29	attach	linked	741:746	arg1	release					818:824	prostacyclin release	805:824	prostacyclin release	805:824	Robust endothelial inflammation induced by LPS was linked to LDs composed of highly unsaturated lipids, as well as prostacyclin release.
34204022	4	30	theme	prostacyclin	805:816	arg1	release					818:824	prostacyclin release	805:824	prostacyclin release	805:824	Robust endothelial inflammation induced by LPS was linked to LDs composed of highly unsaturated lipids, as well as prostacyclin release.
34204022	4	31	theme	endothelial	697:707	arg1	inflammation					709:720	Robust endothelial inflammation	690:720	Robust endothelial inflammation induced by LPS	690:735	Robust endothelial inflammation induced by LPS was linked to LDs composed of highly unsaturated lipids, as well as prostacyclin release.
34204022	6	32	theme	endothelial	994:1004	arg1	inflammation					1006:1017	endothelial inflammation	994:1017	endothelial inflammation induced by LPS	994:1032	In summary, LDs formation represents an integral component of endothelial inflammation induced by LPS.
34204022	4	33	theme	unsaturated	774:784	arg1	lipids					786:791	highly unsaturated lipids	767:791	highly unsaturated lipids	767:791	Robust endothelial inflammation induced by LPS was linked to LDs composed of highly unsaturated lipids, as well as prostacyclin release.
34204022	1	34	theme	pathology	155:163	arg1	hallmark					134:141	the hallmark	130:141	the hallmark of vascular pathology	130:163	Endothelial inflammation is the hallmark of vascular pathology often proceeding with cardiovascular diseases.
34204022	0	35	theme	Inflammation	73:84	arg1	Component					48:56	an Integral Component	36:56	an Integral Component of Endothelial Inflammation Induced by LPS	36:99	Lipid Droplets Formation Represents an Integral Component of Endothelial Inflammation Induced by LPS.
34204022	2	36	theme	imaging	273:279	arg1	techniques					281:290	various imaging techniques	265:290	various imaging techniques	265:290	Here, we adopted a multiparameter approach combining various imaging techniques at the nano- and microscale (Raman, AFM and fluorescence) to investigate endothelial inflammation in response to lipopolysaccharides (LPS) in vitro in human microvascular endothelial cells (HMEC-1) with a focus on lipid droplets (LDs) formation.
34204022	2	37	with	cells	475:479	arg1	focus					497:501	a focus	495:501	a focus on lipid droplets (LDs) formation	495:535	Here, we adopted a multiparameter approach combining various imaging techniques at the nano- and microscale (Raman, AFM and fluorescence) to investigate endothelial inflammation in response to lipopolysaccharides (LPS) in vitro in human microvascular endothelial cells (HMEC-1) with a focus on lipid droplets (LDs) formation.
34204022	1	38	theme	vascular	146:153	arg1	pathology					155:163	vascular pathology	146:163	vascular pathology	146:163	Endothelial inflammation is the hallmark of vascular pathology often proceeding with cardiovascular diseases.
34204022	0	39	theme	Endothelial	61:71	arg1	Inflammation					73:84	Endothelial Inflammation	61:84	Endothelial Inflammation Induced by LPS	61:99	Lipid Droplets Formation Represents an Integral Component of Endothelial Inflammation Induced by LPS.
34204022	2	40	theme	various	265:271	arg1	techniques					281:290	various imaging techniques	265:290	various imaging techniques	265:290	Here, we adopted a multiparameter approach combining various imaging techniques at the nano- and microscale (Raman, AFM and fluorescence) to investigate endothelial inflammation in response to lipopolysaccharides (LPS) in vitro in human microvascular endothelial cells (HMEC-1) with a focus on lipid droplets (LDs) formation.
34204022	2	41	theme	endothelial	463:473	arg1	HMEC-1					482:487	HMEC-1	482:487	HMEC-1	482:487	Here, we adopted a multiparameter approach combining various imaging techniques at the nano- and microscale (Raman, AFM and fluorescence) to investigate endothelial inflammation in response to lipopolysaccharides (LPS) in vitro in human microvascular endothelial cells (HMEC-1) with a focus on lipid droplets (LDs) formation.
34204022	2	41	theme	endothelial	463:473	arg1	cells					475:479	human microvascular endothelial cells	443:479	human microvascular endothelial cells (HMEC-1) with a focus on lipid droplets (LDs) formation	443:535	Here, we adopted a multiparameter approach combining various imaging techniques at the nano- and microscale (Raman, AFM and fluorescence) to investigate endothelial inflammation in response to lipopolysaccharides (LPS) in vitro in human microvascular endothelial cells (HMEC-1) with a focus on lipid droplets (LDs) formation.
34547316	0	0	theme	lavender	99:106	arg1	oil					118:120	lavender essential oil	99:120	lavender essential oil for skin-friendly cellulosic support	99:157	Preparation, characterization, and application of polysaccharide-based emulsions incorporated with lavender essential oil for skin-friendly cellulosic support.
34547316	7	1	theme	healthy	1080:1086	arg1	volunteers					1088:1097	healthy volunteers	1080:1097	healthy volunteers	1080:1097	Obtained cellulosic support was analyzed in terms of active compound controlled release, toxicity and antimicrobial testing, and skin analysis in healthy volunteers.
34547316	7	2	from	release	1014:1020	arg1	volunteers					1088:1097	healthy volunteers	1080:1097	healthy volunteers	1080:1097	Obtained cellulosic support was analyzed in terms of active compound controlled release, toxicity and antimicrobial testing, and skin analysis in healthy volunteers.
34547316	7	3	theme	Obtained	934:941	arg1	support					954:960	Obtained cellulosic support	934:960	Obtained cellulosic support	934:960	Obtained cellulosic support was analyzed in terms of active compound controlled release, toxicity and antimicrobial testing, and skin analysis in healthy volunteers.
34547316	5	4	theme	microbiological	738:752	arg1	analysis					754:761	microbiological analysis	738:761	microbiological analysis	738:761	A total of seven emulsions (RiACL) were evaluated by determining rheological parameters and microbiological analysis.
34547316	4	5	theme	chemical	543:550	arg1	composition					552:562	The chemical composition	539:562	The chemical composition of essential oil	539:579	The chemical composition of essential oil was analyzed using gas chromatography-mass spectrometry (GC/MS).
34547316	7	6	theme	release	1014:1020	arg1	terms					978:982	terms	978:982	terms of active compound controlled release, toxicity and antimicrobial testing, and skin analysis in healthy volunteers	978:1097	Obtained cellulosic support was analyzed in terms of active compound controlled release, toxicity and antimicrobial testing, and skin analysis in healthy volunteers.
34547316	2	7	theme	active	388:393	arg1	compound					395:402	an active compound	385:402	an active compound mainly due to its antimicrobial properties	385:445	The lavender essential oil has been added to emulsions as an active compound mainly due to its antimicrobial properties.
34547316	4	8	theme	chromatography-mass	604:622	arg1	GC/MS					638:642	GC/MS	638:642	GC/MS	638:642	The chemical composition of essential oil was analyzed using gas chromatography-mass spectrometry (GC/MS).
34547316	4	8	theme	chromatography-mass	604:622	arg1	spectrometry					624:635	gas chromatography-mass spectrometry	600:635	gas chromatography-mass spectrometry (GC/MS)	600:643	The chemical composition of essential oil was analyzed using gas chromatography-mass spectrometry (GC/MS).
34547316	8	9	theme	moisturizing	1207:1218	arg1	effects					1220:1226	moisturizing effects	1207:1226	moisturizing effects	1207:1226	It was found that the cellulosic supports treated with O/W emulsions are non-irritating, have softness and moisturizing effects, and can be used safely in topical applications for patches obtaining.
34547316	7	10	theme	active	987:992	arg1	release					1014:1020	active compound controlled release	987:1020	active compound controlled release	987:1020	Obtained cellulosic support was analyzed in terms of active compound controlled release, toxicity and antimicrobial testing, and skin analysis in healthy volunteers.
34547316	6	11	theme	non-irritating	837:850	arg1	textiles					852:859	non-irritating textiles	837:859	non-irritating textiles	837:859	One of the emulsions (R7ACL) was applied to cellulosic support to obtain non-irritating textiles with controlled release of the active compound and moisturizing effects.
34547316	2	12	theme	essential	340:348	arg1	oil					350:352	The lavender essential oil	327:352	The lavender essential oil	327:352	The lavender essential oil has been added to emulsions as an active compound mainly due to its antimicrobial properties.
34547316	0	13	theme	essential	108:116	arg1	oil					118:120	lavender essential oil	99:120	lavender essential oil for skin-friendly cellulosic support	99:157	Preparation, characterization, and application of polysaccharide-based emulsions incorporated with lavender essential oil for skin-friendly cellulosic support.
34547316	7	14	theme	compound	994:1001	arg1	release					1014:1020	active compound controlled release	987:1020	active compound controlled release	987:1020	Obtained cellulosic support was analyzed in terms of active compound controlled release, toxicity and antimicrobial testing, and skin analysis in healthy volunteers.
34547316	2	15	theme	lavender	331:338	arg1	oil					350:352	The lavender essential oil	327:352	The lavender essential oil	327:352	The lavender essential oil has been added to emulsions as an active compound mainly due to its antimicrobial properties.
34547316	1	16	theme	obtaining	316:324	arg1	patches					308:314	patches obtaining	308:324	patches obtaining	308:324	This study aimed to develop polysaccharide-based emulsions incorporated with lavender essential oil and their application on cellulosic support for patches obtaining.
34547316	0	17	theme	skin-friendly	126:138	arg1	support					151:157	skin-friendly cellulosic support	126:157	skin-friendly cellulosic support	126:157	Preparation, characterization, and application of polysaccharide-based emulsions incorporated with lavender essential oil for skin-friendly cellulosic support.
34547316	7	18	theme	controlled	1003:1012	arg1	release					1014:1020	active compound controlled release	987:1020	active compound controlled release	987:1020	Obtained cellulosic support was analyzed in terms of active compound controlled release, toxicity and antimicrobial testing, and skin analysis in healthy volunteers.
34547316	3	19	used	used	478:481	arg2	emulsions					463:471	emulsions	463:471	emulsions	463:471	In this study, emulsions were used to deliver active ingredients (lavender essential oil).
34547316	1	20	theme	polysaccharide-based	188:207	arg1	emulsions					209:217	polysaccharide-based emulsions	188:217	polysaccharide-based emulsions incorporated with lavender essential oil and their application on cellulosic support for patches obtaining	188:324	This study aimed to develop polysaccharide-based emulsions incorporated with lavender essential oil and their application on cellulosic support for patches obtaining.
34547316	6	21	theme	effects	925:931	arg1	release					877:883	controlled release	866:883	controlled release of the active compound and moisturizing effects	866:931	One of the emulsions (R7ACL) was applied to cellulosic support to obtain non-irritating textiles with controlled release of the active compound and moisturizing effects.
34547316	2	22	theme	antimicrobial	422:434	arg1	properties					436:445	its antimicrobial properties	418:445	its antimicrobial properties	418:445	The lavender essential oil has been added to emulsions as an active compound mainly due to its antimicrobial properties.
34547316	6	23	theme	moisturizing	912:923	arg1	effects					925:931	moisturizing effects	912:931	moisturizing effects	912:931	One of the emulsions (R7ACL) was applied to cellulosic support to obtain non-irritating textiles with controlled release of the active compound and moisturizing effects.
34547316	5	24	theme	emulsions	663:671	arg1	total					648:652	A total	646:652	A total of seven emulsions (RiACL)	646:679	A total of seven emulsions (RiACL) were evaluated by determining rheological parameters and microbiological analysis.
34547316	6	25	theme	cellulosic	808:817	arg1	support					819:825	cellulosic support	808:825	cellulosic support	808:825	One of the emulsions (R7ACL) was applied to cellulosic support to obtain non-irritating textiles with controlled release of the active compound and moisturizing effects.
34547316	8	26	contain	have	1189:1192	arg1	non-irritating					1173:1186	non-irritating	1173:1186	non-irritating	1173:1186	It was found that the cellulosic supports treated with O/W emulsions are non-irritating, have softness and moisturizing effects, and can be used safely in topical applications for patches obtaining.
34547316	8	26	contain	have	1189:1192	arg2	effects					1220:1226	moisturizing effects	1207:1226	moisturizing effects	1207:1226	It was found that the cellulosic supports treated with O/W emulsions are non-irritating, have softness and moisturizing effects, and can be used safely in topical applications for patches obtaining.
34547316	8	26	contain	have	1189:1192	arg2	softness					1194:1201	softness	1194:1201	softness	1194:1201	It was found that the cellulosic supports treated with O/W emulsions are non-irritating, have softness and moisturizing effects, and can be used safely in topical applications for patches obtaining.
34547316	8	26	contain	have	1189:1192	arg1	cellulosic					1122:1131	cellulosic	1122:1131	cellulosic	1122:1131	It was found that the cellulosic supports treated with O/W emulsions are non-irritating, have softness and moisturizing effects, and can be used safely in topical applications for patches obtaining.
34547316	7	27	from	testing	1050:1056	arg1	volunteers					1088:1097	healthy volunteers	1080:1097	healthy volunteers	1080:1097	Obtained cellulosic support was analyzed in terms of active compound controlled release, toxicity and antimicrobial testing, and skin analysis in healthy volunteers.
34547316	3	28	theme	active	494:499	arg1	oil					533:535	lavender essential oil	514:535	lavender essential oil	514:535	In this study, emulsions were used to deliver active ingredients (lavender essential oil).
34547316	3	28	theme	active	494:499	arg1	ingredients					501:511	active ingredients	494:511	active ingredients (lavender essential oil)	494:536	In this study, emulsions were used to deliver active ingredients (lavender essential oil).
34547316	4	29	theme	gas	600:602	arg1	GC/MS					638:642	GC/MS	638:642	GC/MS	638:642	The chemical composition of essential oil was analyzed using gas chromatography-mass spectrometry (GC/MS).
34547316	4	29	theme	gas	600:602	arg1	spectrometry					624:635	gas chromatography-mass spectrometry	600:635	gas chromatography-mass spectrometry (GC/MS)	600:643	The chemical composition of essential oil was analyzed using gas chromatography-mass spectrometry (GC/MS).
34547316	0	30	theme	cellulosic	140:149	arg1	support					151:157	skin-friendly cellulosic support	126:157	skin-friendly cellulosic support	126:157	Preparation, characterization, and application of polysaccharide-based emulsions incorporated with lavender essential oil for skin-friendly cellulosic support.
34547316	1	31	theme	essential	246:254	arg1	oil					256:258	lavender essential oil	237:258	lavender essential oil	237:258	This study aimed to develop polysaccharide-based emulsions incorporated with lavender essential oil and their application on cellulosic support for patches obtaining.
34547316	6	32	theme	compound	899:906	arg1	release					877:883	controlled release	866:883	controlled release of the active compound and moisturizing effects	866:931	One of the emulsions (R7ACL) was applied to cellulosic support to obtain non-irritating textiles with controlled release of the active compound and moisturizing effects.
34547316	7	33	from	toxicity	1023:1030	arg1	volunteers					1088:1097	healthy volunteers	1080:1097	healthy volunteers	1080:1097	Obtained cellulosic support was analyzed in terms of active compound controlled release, toxicity and antimicrobial testing, and skin analysis in healthy volunteers.
34547316	8	34	used	used	1240:1243	arg2	non-irritating					1173:1186	non-irritating	1173:1186	non-irritating	1173:1186	It was found that the cellulosic supports treated with O/W emulsions are non-irritating, have softness and moisturizing effects, and can be used safely in topical applications for patches obtaining.
34547316	8	34	used	used	1240:1243	arg2	cellulosic					1122:1131	cellulosic	1122:1131	cellulosic	1122:1131	It was found that the cellulosic supports treated with O/W emulsions are non-irritating, have softness and moisturizing effects, and can be used safely in topical applications for patches obtaining.
34547316	8	35	dep	supports	1133:1140	arg1	treated					1142:1148	treated	1142:1148	supports treated with O/W emulsions	1133:1167	It was found that the cellulosic supports treated with O/W emulsions are non-irritating, have softness and moisturizing effects, and can be used safely in topical applications for patches obtaining.
34547316	6	36	theme	active	892:897	arg1	compound					899:906	the active compound	888:906	the active compound	888:906	One of the emulsions (R7ACL) was applied to cellulosic support to obtain non-irritating textiles with controlled release of the active compound and moisturizing effects.
34547316	1	37	from	oil	256:258	arg1	support					296:302	cellulosic support	285:302	cellulosic support for patches obtaining	285:324	This study aimed to develop polysaccharide-based emulsions incorporated with lavender essential oil and their application on cellulosic support for patches obtaining.
34547316	7	38	theme	antimicrobial	1036:1048	arg1	testing					1050:1056	antimicrobial testing	1036:1056	antimicrobial testing	1036:1056	Obtained cellulosic support was analyzed in terms of active compound controlled release, toxicity and antimicrobial testing, and skin analysis in healthy volunteers.
34547316	0	39	theme	polysaccharide-based	50:69	arg1	emulsions					71:79	polysaccharide-based emulsions	50:79	polysaccharide-based emulsions	50:79	Preparation, characterization, and application of polysaccharide-based emulsions incorporated with lavender essential oil for skin-friendly cellulosic support.
34547316	8	40	theme	O/W	1155:1157	arg1	emulsions					1159:1167	O/W emulsions	1155:1167	O/W emulsions	1155:1167	It was found that the cellulosic supports treated with O/W emulsions are non-irritating, have softness and moisturizing effects, and can be used safely in topical applications for patches obtaining.
34547316	7	41	theme	analysis	1068:1075	arg1	terms					978:982	terms	978:982	terms of active compound controlled release, toxicity and antimicrobial testing, and skin analysis in healthy volunteers	978:1097	Obtained cellulosic support was analyzed in terms of active compound controlled release, toxicity and antimicrobial testing, and skin analysis in healthy volunteers.
34547316	3	42	theme	essential	523:531	arg1	oil					533:535	lavender essential oil	514:535	lavender essential oil	514:535	In this study, emulsions were used to deliver active ingredients (lavender essential oil).
34547316	3	42	theme	essential	523:531	arg1	ingredients					501:511	active ingredients	494:511	active ingredients (lavender essential oil)	494:536	In this study, emulsions were used to deliver active ingredients (lavender essential oil).
34547316	8	43	theme	topical	1255:1261	arg1	applications					1263:1274	topical applications	1255:1274	topical applications	1255:1274	It was found that the cellulosic supports treated with O/W emulsions are non-irritating, have softness and moisturizing effects, and can be used safely in topical applications for patches obtaining.
34547316	4	44	theme	oil	577:579	arg1	composition					552:562	The chemical composition	539:562	The chemical composition of essential oil	539:579	The chemical composition of essential oil was analyzed using gas chromatography-mass spectrometry (GC/MS).
34547316	7	45	from	terms	978:982	arg1	volunteers					1088:1097	healthy volunteers	1080:1097	healthy volunteers	1080:1097	Obtained cellulosic support was analyzed in terms of active compound controlled release, toxicity and antimicrobial testing, and skin analysis in healthy volunteers.
34547316	4	46	theme	essential	567:575	arg1	oil					577:579	essential oil	567:579	essential oil	567:579	The chemical composition of essential oil was analyzed using gas chromatography-mass spectrometry (GC/MS).
34547316	5	47	theme	rheological	711:721	arg1	parameters					723:732	rheological parameters	711:732	rheological parameters	711:732	A total of seven emulsions (RiACL) were evaluated by determining rheological parameters and microbiological analysis.
34547316	1	48	from	application	270:280	arg1	support					296:302	cellulosic support	285:302	cellulosic support for patches obtaining	285:324	This study aimed to develop polysaccharide-based emulsions incorporated with lavender essential oil and their application on cellulosic support for patches obtaining.
34547316	6	49	theme	controlled	866:875	arg1	release					877:883	controlled release	866:883	controlled release of the active compound and moisturizing effects	866:931	One of the emulsions (R7ACL) was applied to cellulosic support to obtain non-irritating textiles with controlled release of the active compound and moisturizing effects.
34547316	7	50	theme	skin	1063:1066	arg1	analysis					1068:1075	skin analysis	1063:1075	skin analysis	1063:1075	Obtained cellulosic support was analyzed in terms of active compound controlled release, toxicity and antimicrobial testing, and skin analysis in healthy volunteers.
34547316	6	51	theme	emulsions	775:783	arg1	R7ACL					786:790	R7ACL	786:790	R7ACL	786:790	One of the emulsions (R7ACL) was applied to cellulosic support to obtain non-irritating textiles with controlled release of the active compound and moisturizing effects.
34547316	6	51	theme	emulsions	775:783	arg1	One					764:766	One	764:766	One	764:766	One of the emulsions (R7ACL) was applied to cellulosic support to obtain non-irritating textiles with controlled release of the active compound and moisturizing effects.
34547316	6	51	theme	emulsions	775:783	arg1	emulsions					775:783	the emulsions	771:783	the emulsions (R7ACL)	771:791	One of the emulsions (R7ACL) was applied to cellulosic support to obtain non-irritating textiles with controlled release of the active compound and moisturizing effects.
34547316	3	52	theme	lavender	514:521	arg1	oil					533:535	lavender essential oil	514:535	lavender essential oil	514:535	In this study, emulsions were used to deliver active ingredients (lavender essential oil).
34547316	3	52	theme	lavender	514:521	arg1	ingredients					501:511	active ingredients	494:511	active ingredients (lavender essential oil)	494:536	In this study, emulsions were used to deliver active ingredients (lavender essential oil).
34547316	7	53	theme	cellulosic	943:952	arg1	support					954:960	Obtained cellulosic support	934:960	Obtained cellulosic support	934:960	Obtained cellulosic support was analyzed in terms of active compound controlled release, toxicity and antimicrobial testing, and skin analysis in healthy volunteers.
34547316	7	54	from	analysis	1068:1075	arg1	volunteers					1088:1097	healthy volunteers	1080:1097	healthy volunteers	1080:1097	Obtained cellulosic support was analyzed in terms of active compound controlled release, toxicity and antimicrobial testing, and skin analysis in healthy volunteers.
34547316	1	55	theme	lavender	237:244	arg1	oil					256:258	lavender essential oil	237:258	lavender essential oil	237:258	This study aimed to develop polysaccharide-based emulsions incorporated with lavender essential oil and their application on cellulosic support for patches obtaining.
34547316	2	56	theme	due	411:413	arg1	compound					395:402	an active compound	385:402	an active compound mainly due to its antimicrobial properties	385:445	The lavender essential oil has been added to emulsions as an active compound mainly due to its antimicrobial properties.
34547316	7	57	from	volunteers	1088:1097	arg1	terms					978:982	terms	978:982	terms of active compound controlled release, toxicity and antimicrobial testing, and skin analysis in healthy volunteers	978:1097	Obtained cellulosic support was analyzed in terms of active compound controlled release, toxicity and antimicrobial testing, and skin analysis in healthy volunteers.
34547316	0	58	theme	emulsions	71:79	arg1	characterization					13:28	characterization	13:28	characterization	13:28	Preparation, characterization, and application of polysaccharide-based emulsions incorporated with lavender essential oil for skin-friendly cellulosic support.
34547316	0	58	theme	emulsions	71:79	arg1	application					35:45	application	35:45	application	35:45	Preparation, characterization, and application of polysaccharide-based emulsions incorporated with lavender essential oil for skin-friendly cellulosic support.
34547316	0	58	theme	emulsions	71:79	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation, characterization, and application of polysaccharide-based emulsions incorporated with lavender essential oil for skin-friendly cellulosic support.
34547316	7	59	theme	toxicity	1023:1030	arg1	terms					978:982	terms	978:982	terms of active compound controlled release, toxicity and antimicrobial testing, and skin analysis in healthy volunteers	978:1097	Obtained cellulosic support was analyzed in terms of active compound controlled release, toxicity and antimicrobial testing, and skin analysis in healthy volunteers.
34547316	8	60	dep	cellulosic	1122:1131	arg1	supports					1133:1140	supports	1133:1140	supports treated with O/W emulsions	1133:1167	It was found that the cellulosic supports treated with O/W emulsions are non-irritating, have softness and moisturizing effects, and can be used safely in topical applications for patches obtaining.
34547316	1	61	theme	cellulosic	285:294	arg1	support					296:302	cellulosic support	285:302	cellulosic support for patches obtaining	285:324	This study aimed to develop polysaccharide-based emulsions incorporated with lavender essential oil and their application on cellulosic support for patches obtaining.
32892290	11	0	theme	consumed	1584:1591	arg1	fruit					1602:1606	traditionally consumed bilberry fruit	1570:1606	traditionally consumed bilberry fruit	1570:1606	Finally, it was shown that only trace amounts of free growth regulators are present in the cells at the harvesting point and assessment by freshwater crustaceans assay indicated that toxicity of the cells was not exceeding that of traditionally consumed bilberry fruit.
32892290	10	1	theme	Synthetic	1207:1215	arg1	regulators					1230:1239	Synthetic plant growth regulators	1207:1239	Synthetic plant growth regulators	1207:1239	Synthetic plant growth regulators were replaced by natural components, such as coconut water and IAA for several subculture cycles.
32892290	7	2	theme	plant	715:719	arg1	cultures					726:733	two plant cell cultures	711:733	two plant cell cultures	711:733	In this study, two plant cell cultures, deriving from arctic bramble (Rubus arcticus) and birch (Betula pendula), were cultivated using lactose-rich dairy side streams as alternative carbon sources to replace sucrose.
32892290	8	3	theme	arctic	1072:1077	arg1	bramble					1079:1085	arctic bramble	1072:1085	arctic bramble	1072:1085	Biomasses were comparable to those of original plant cell culture media when up to 83% and 75% of the original sucrose was replaced by these side streams for arctic bramble and birch cell cultures, respectively.
32892290	4	4	theme	Plant	407:411	arg1	cultures					418:425	Plant cell cultures	407:425	Plant cell cultures used for food	407:439	Plant cell cultures used for food could supplement current food production.
32892290	7	5	theme	lactose-rich	832:843	arg1	streams					856:862	lactose-rich dairy side streams	832:862	lactose-rich dairy side streams	832:862	In this study, two plant cell cultures, deriving from arctic bramble (Rubus arcticus) and birch (Betula pendula), were cultivated using lactose-rich dairy side streams as alternative carbon sources to replace sucrose.
32892290	7	5	theme	lactose-rich	832:843	arg1	sources					886:892	alternative carbon sources	867:892	alternative carbon sources	867:892	In this study, two plant cell cultures, deriving from arctic bramble (Rubus arcticus) and birch (Betula pendula), were cultivated using lactose-rich dairy side streams as alternative carbon sources to replace sucrose.
32892290	4	6	theme	current	458:464	arg1	production					471:480	current food production	458:480	current food production	458:480	Plant cell cultures used for food could supplement current food production.
32892290	8	7	theme	birch	1091:1095	arg1	cultures					1102:1109	birch cell cultures	1091:1109	birch cell cultures	1091:1109	Biomasses were comparable to those of original plant cell culture media when up to 83% and 75% of the original sucrose was replaced by these side streams for arctic bramble and birch cell cultures, respectively.
32892290	7	8	attach	deriving	736:743	arg1	bramble					757:763	arctic bramble	750:763	arctic bramble (Rubus arcticus)	750:780	In this study, two plant cell cultures, deriving from arctic bramble (Rubus arcticus) and birch (Betula pendula), were cultivated using lactose-rich dairy side streams as alternative carbon sources to replace sucrose.
32892290	7	8	attach	deriving	736:743	arg2	cultures					726:733	two plant cell cultures	711:733	two plant cell cultures	711:733	In this study, two plant cell cultures, deriving from arctic bramble (Rubus arcticus) and birch (Betula pendula), were cultivated using lactose-rich dairy side streams as alternative carbon sources to replace sucrose.
32892290	7	8	attach	deriving	736:743	arg1	birch					786:790	birch	786:790	birch (Betula pendula)	786:807	In this study, two plant cell cultures, deriving from arctic bramble (Rubus arcticus) and birch (Betula pendula), were cultivated using lactose-rich dairy side streams as alternative carbon sources to replace sucrose.
32892290	10	9	theme	subculture	1320:1329	arg1	cycles					1331:1336	several subculture cycles	1312:1336	several subculture cycles	1312:1336	Synthetic plant growth regulators were replaced by natural components, such as coconut water and IAA for several subculture cycles.
32892290	11	10	from	cells	1430:1434	arg1	present					1415:1421	present	1415:1421	present	1415:1421	Finally, it was shown that only trace amounts of free growth regulators are present in the cells at the harvesting point and assessment by freshwater crustaceans assay indicated that toxicity of the cells was not exceeding that of traditionally consumed bilberry fruit.
32892290	7	11	theme	dairy	845:849	arg1	streams					856:862	lactose-rich dairy side streams	832:862	lactose-rich dairy side streams	832:862	In this study, two plant cell cultures, deriving from arctic bramble (Rubus arcticus) and birch (Betula pendula), were cultivated using lactose-rich dairy side streams as alternative carbon sources to replace sucrose.
32892290	7	11	theme	dairy	845:849	arg1	sources					886:892	alternative carbon sources	867:892	alternative carbon sources	867:892	In this study, two plant cell cultures, deriving from arctic bramble (Rubus arcticus) and birch (Betula pendula), were cultivated using lactose-rich dairy side streams as alternative carbon sources to replace sucrose.
32892290	8	12	dep	83	997:998	arg1	to					994:995	to	994:995	to	994:995	Biomasses were comparable to those of original plant cell culture media when up to 83% and 75% of the original sucrose was replaced by these side streams for arctic bramble and birch cell cultures, respectively.
32892290	8	13	theme	plant	961:965	arg1	media					980:984	original plant cell culture media	952:984	original plant cell culture media	952:984	Biomasses were comparable to those of original plant cell culture media when up to 83% and 75% of the original sucrose was replaced by these side streams for arctic bramble and birch cell cultures, respectively.
32892290	5	14	theme	many	498:501	arg1	aspects					503:509	many aspects	498:509	many aspects	498:509	However, still many aspects need to be resolved before this new food concept can enter the market.
32892290	7	15	dep	bramble	757:763	arg1	arcticus					772:779	Rubus arcticus	766:779	Rubus arcticus	766:779	In this study, two plant cell cultures, deriving from arctic bramble (Rubus arcticus) and birch (Betula pendula), were cultivated using lactose-rich dairy side streams as alternative carbon sources to replace sucrose.
32892290	4	16	theme	cell	413:416	arg1	cultures					418:425	Plant cell cultures	407:425	Plant cell cultures used for food	407:439	Plant cell cultures used for food could supplement current food production.
32892290	1	17	theme	Sustainability	66:79	arg1	aspects					92:98	Sustainability and safety aspects	66:98	Sustainability and safety aspects of plant cell cultures as food	66:129	Sustainability and safety aspects of plant cell cultures as food are presented.
32892290	2	18	from	use	203:205	arg1	cultivation					238:248	cultivation	238:248	cultivation	238:248	Applicability of dairy side streams as carbon source and use of natural growth enhancers in cultivation are shown.
32892290	8	19	theme	cell	1097:1100	arg1	cultures					1102:1109	birch cell cultures	1091:1109	birch cell cultures	1091:1109	Biomasses were comparable to those of original plant cell culture media when up to 83% and 75% of the original sucrose was replaced by these side streams for arctic bramble and birch cell cultures, respectively.
32892290	11	20	attach	present	1415:1421	arg2	regulators					1400:1409	free growth regulators	1388:1409	free growth regulators	1388:1409	Finally, it was shown that only trace amounts of free growth regulators are present in the cells at the harvesting point and assessment by freshwater crustaceans assay indicated that toxicity of the cells was not exceeding that of traditionally consumed bilberry fruit.
32892290	11	20	attach	present	1415:1421	arg1	point					1454:1458	the harvesting point	1439:1458	the harvesting point	1439:1458	Finally, it was shown that only trace amounts of free growth regulators are present in the cells at the harvesting point and assessment by freshwater crustaceans assay indicated that toxicity of the cells was not exceeding that of traditionally consumed bilberry fruit.
32892290	11	20	attach	present	1415:1421	arg2	amounts					1377:1383	only trace amounts	1366:1383	only trace amounts of free growth regulators	1366:1409	Finally, it was shown that only trace amounts of free growth regulators are present in the cells at the harvesting point and assessment by freshwater crustaceans assay indicated that toxicity of the cells was not exceeding that of traditionally consumed bilberry fruit.
32892290	11	20	attach	present	1415:1421	arg1	cells					1430:1434	the cells	1426:1434	the cells	1426:1434	Finally, it was shown that only trace amounts of free growth regulators are present in the cells at the harvesting point and assessment by freshwater crustaceans assay indicated that toxicity of the cells was not exceeding that of traditionally consumed bilberry fruit.
32892290	9	21	theme	nutritional	1139:1149	arg1	composition					1151:1161	nutritional composition	1139:1161	nutritional composition	1139:1161	Furthermore, nutritional composition or sensory properties were not compromised.
32892290	8	22	theme	culture	972:978	arg1	media					980:984	original plant cell culture media	952:984	original plant cell culture media	952:984	Biomasses were comparable to those of original plant cell culture media when up to 83% and 75% of the original sucrose was replaced by these side streams for arctic bramble and birch cell cultures, respectively.
32892290	3	23	theme	commodities	394:404	arg1	portfolio					358:366	the portfolio	354:366	the portfolio of agriculturally derived commodities	354:404	Biotechnologically produced cellular products are currently emerging to replace and add into the portfolio of agriculturally derived commodities.
32892290	7	24	dep	birch	786:790	arg1	pendula					800:806	Betula pendula	793:806	Betula pendula	793:806	In this study, two plant cell cultures, deriving from arctic bramble (Rubus arcticus) and birch (Betula pendula), were cultivated using lactose-rich dairy side streams as alternative carbon sources to replace sucrose.
32892290	0	25	theme	cell	6:9	arg1	cultures					11:18	Plant cell cultures	0:18	Plant cell cultures	0:18	Plant cell cultures as food-aspects of sustainability and safety.
32892290	8	26	theme	cell	967:970	arg1	media					980:984	original plant cell culture media	952:984	original plant cell culture media	952:984	Biomasses were comparable to those of original plant cell culture media when up to 83% and 75% of the original sucrose was replaced by these side streams for arctic bramble and birch cell cultures, respectively.
32892290	1	27	theme	safety	85:90	arg1	aspects					92:98	Sustainability and safety aspects	66:98	Sustainability and safety aspects of plant cell cultures as food	66:129	Sustainability and safety aspects of plant cell cultures as food are presented.
32892290	10	28	theme	growth	1223:1228	arg1	regulators					1230:1239	Synthetic plant growth regulators	1207:1239	Synthetic plant growth regulators	1207:1239	Synthetic plant growth regulators were replaced by natural components, such as coconut water and IAA for several subculture cycles.
32892290	0	29	theme	Plant	0:4	arg1	cultures					11:18	Plant cell cultures	0:18	Plant cell cultures	0:18	Plant cell cultures as food-aspects of sustainability and safety.
32892290	11	30	theme	freshwater	1478:1487	arg1	assay					1501:1505	freshwater crustaceans assay	1478:1505	freshwater crustaceans assay	1478:1505	Finally, it was shown that only trace amounts of free growth regulators are present in the cells at the harvesting point and assessment by freshwater crustaceans assay indicated that toxicity of the cells was not exceeding that of traditionally consumed bilberry fruit.
32892290	10	31	theme	plant	1217:1221	arg1	regulators					1230:1239	Synthetic plant growth regulators	1207:1239	Synthetic plant growth regulators	1207:1239	Synthetic plant growth regulators were replaced by natural components, such as coconut water and IAA for several subculture cycles.
32892290	11	32	theme	trace	1371:1375	arg1	amounts					1377:1383	only trace amounts	1366:1383	only trace amounts of free growth regulators	1366:1409	Finally, it was shown that only trace amounts of free growth regulators are present in the cells at the harvesting point and assessment by freshwater crustaceans assay indicated that toxicity of the cells was not exceeding that of traditionally consumed bilberry fruit.
32892290	11	32	theme	trace	1371:1375	arg1	regulators					1400:1409	free growth regulators	1388:1409	free growth regulators	1388:1409	Finally, it was shown that only trace amounts of free growth regulators are present in the cells at the harvesting point and assessment by freshwater crustaceans assay indicated that toxicity of the cells was not exceeding that of traditionally consumed bilberry fruit.
32892290	6	33	theme	related	589:595	arg1	Issues					582:587	Issues	582:587	Issues related to sustainability and safety for human consumption	582:646	Issues related to sustainability and safety for human consumption are relevant for both consumers and regulators.
32892290	8	34	theme	sucrose	1025:1031	arg1	sucrose					1025:1031	the original sucrose	1012:1031	the original sucrose	1012:1031	Biomasses were comparable to those of original plant cell culture media when up to 83% and 75% of the original sucrose was replaced by these side streams for arctic bramble and birch cell cultures, respectively.
32892290	8	34	theme	sucrose	1025:1031	arg1	%					999:999	up to 83%	991:999	up to 83%	991:999	Biomasses were comparable to those of original plant cell culture media when up to 83% and 75% of the original sucrose was replaced by these side streams for arctic bramble and birch cell cultures, respectively.
32892290	8	34	theme	sucrose	1025:1031	arg1	%					1007:1007	75%	1005:1007	75% of the original sucrose	1005:1031	Biomasses were comparable to those of original plant cell culture media when up to 83% and 75% of the original sucrose was replaced by these side streams for arctic bramble and birch cell cultures, respectively.
32892290	8	35	theme	original	1016:1023	arg1	sucrose					1025:1031	the original sucrose	1012:1031	the original sucrose	1012:1031	Biomasses were comparable to those of original plant cell culture media when up to 83% and 75% of the original sucrose was replaced by these side streams for arctic bramble and birch cell cultures, respectively.
32892290	8	36	theme	original	952:959	arg1	media					980:984	original plant cell culture media	952:984	original plant cell culture media	952:984	Biomasses were comparable to those of original plant cell culture media when up to 83% and 75% of the original sucrose was replaced by these side streams for arctic bramble and birch cell cultures, respectively.
32892290	2	37	from	enhancers	225:233	arg1	cultivation					238:248	cultivation	238:248	cultivation	238:248	Applicability of dairy side streams as carbon source and use of natural growth enhancers in cultivation are shown.
32892290	11	38	located	present	1415:1421	arg2	regulators					1400:1409	free growth regulators	1388:1409	free growth regulators	1388:1409	Finally, it was shown that only trace amounts of free growth regulators are present in the cells at the harvesting point and assessment by freshwater crustaceans assay indicated that toxicity of the cells was not exceeding that of traditionally consumed bilberry fruit.
32892290	11	38	located	present	1415:1421	arg1	point					1454:1458	the harvesting point	1439:1458	the harvesting point	1439:1458	Finally, it was shown that only trace amounts of free growth regulators are present in the cells at the harvesting point and assessment by freshwater crustaceans assay indicated that toxicity of the cells was not exceeding that of traditionally consumed bilberry fruit.
32892290	11	38	located	present	1415:1421	arg2	amounts					1377:1383	only trace amounts	1366:1383	only trace amounts of free growth regulators	1366:1409	Finally, it was shown that only trace amounts of free growth regulators are present in the cells at the harvesting point and assessment by freshwater crustaceans assay indicated that toxicity of the cells was not exceeding that of traditionally consumed bilberry fruit.
32892290	11	38	located	present	1415:1421	arg1	cells					1430:1434	the cells	1426:1434	the cells	1426:1434	Finally, it was shown that only trace amounts of free growth regulators are present in the cells at the harvesting point and assessment by freshwater crustaceans assay indicated that toxicity of the cells was not exceeding that of traditionally consumed bilberry fruit.
32892290	11	39	theme	harvesting	1443:1452	arg1	point					1454:1458	the harvesting point	1439:1458	the harvesting point	1439:1458	Finally, it was shown that only trace amounts of free growth regulators are present in the cells at the harvesting point and assessment by freshwater crustaceans assay indicated that toxicity of the cells was not exceeding that of traditionally consumed bilberry fruit.
32892290	3	40	theme	produced	280:287	arg1	products					298:305	Biotechnologically produced cellular products	261:305	Biotechnologically produced cellular products	261:305	Biotechnologically produced cellular products are currently emerging to replace and add into the portfolio of agriculturally derived commodities.
32892290	2	41	theme	streams	174:180	arg1	use					203:205	use	203:205	use of natural growth enhancers in cultivation	203:248	Applicability of dairy side streams as carbon source and use of natural growth enhancers in cultivation are shown.
32892290	2	41	theme	streams	174:180	arg1	Applicability					146:158	Applicability	146:158	Applicability of dairy side streams as carbon source	146:197	Applicability of dairy side streams as carbon source and use of natural growth enhancers in cultivation are shown.
32892290	1	42	theme	plant	103:107	arg1	cultures					114:121	plant cell cultures	103:121	plant cell cultures	103:121	Sustainability and safety aspects of plant cell cultures as food are presented.
32892290	10	43	theme	natural	1258:1264	arg1	coconut					1286:1292	coconut	1286:1292	coconut	1286:1292	Synthetic plant growth regulators were replaced by natural components, such as coconut water and IAA for several subculture cycles.
32892290	10	43	theme	natural	1258:1264	arg1	IAA					1304:1306	IAA	1304:1306	IAA	1304:1306	Synthetic plant growth regulators were replaced by natural components, such as coconut water and IAA for several subculture cycles.
32892290	10	43	theme	natural	1258:1264	arg1	components					1266:1275	natural components	1258:1275	natural components	1258:1275	Synthetic plant growth regulators were replaced by natural components, such as coconut water and IAA for several subculture cycles.
32892290	11	44	theme	growth	1393:1398	arg1	regulators					1400:1409	free growth regulators	1388:1409	free growth regulators	1388:1409	Finally, it was shown that only trace amounts of free growth regulators are present in the cells at the harvesting point and assessment by freshwater crustaceans assay indicated that toxicity of the cells was not exceeding that of traditionally consumed bilberry fruit.
32892290	11	45	theme	free	1388:1391	arg1	regulators					1400:1409	free growth regulators	1388:1409	free growth regulators	1388:1409	Finally, it was shown that only trace amounts of free growth regulators are present in the cells at the harvesting point and assessment by freshwater crustaceans assay indicated that toxicity of the cells was not exceeding that of traditionally consumed bilberry fruit.
32892290	11	46	theme	bilberry	1593:1600	arg1	fruit					1602:1606	traditionally consumed bilberry fruit	1570:1606	traditionally consumed bilberry fruit	1570:1606	Finally, it was shown that only trace amounts of free growth regulators are present in the cells at the harvesting point and assessment by freshwater crustaceans assay indicated that toxicity of the cells was not exceeding that of traditionally consumed bilberry fruit.
32892290	2	47	theme	side	169:172	arg1	streams					174:180	dairy side streams	163:180	dairy side streams as carbon source	163:197	Applicability of dairy side streams as carbon source and use of natural growth enhancers in cultivation are shown.
32892290	2	48	theme	growth	218:223	arg1	enhancers					225:233	natural growth enhancers	210:233	natural growth enhancers in cultivation	210:248	Applicability of dairy side streams as carbon source and use of natural growth enhancers in cultivation are shown.
32892290	2	49	from	cultivation	238:248	arg1	use					203:205	use	203:205	use of natural growth enhancers in cultivation	203:248	Applicability of dairy side streams as carbon source and use of natural growth enhancers in cultivation are shown.
32892290	2	49	from	cultivation	238:248	arg1	Applicability					146:158	Applicability	146:158	Applicability of dairy side streams as carbon source	146:197	Applicability of dairy side streams as carbon source and use of natural growth enhancers in cultivation are shown.
32892290	8	50	theme	side	1055:1058	arg1	streams					1060:1066	these side streams	1049:1066	these side streams for arctic bramble	1049:1085	Biomasses were comparable to those of original plant cell culture media when up to 83% and 75% of the original sucrose was replaced by these side streams for arctic bramble and birch cell cultures, respectively.
32892290	7	51	theme	cell	721:724	arg1	cultures					726:733	two plant cell cultures	711:733	two plant cell cultures	711:733	In this study, two plant cell cultures, deriving from arctic bramble (Rubus arcticus) and birch (Betula pendula), were cultivated using lactose-rich dairy side streams as alternative carbon sources to replace sucrose.
32892290	2	52	theme	dairy	163:167	arg1	streams					174:180	dairy side streams	163:180	dairy side streams as carbon source	163:197	Applicability of dairy side streams as carbon source and use of natural growth enhancers in cultivation are shown.
32892290	2	53	theme	natural	210:216	arg1	enhancers					225:233	natural growth enhancers	210:233	natural growth enhancers in cultivation	210:248	Applicability of dairy side streams as carbon source and use of natural growth enhancers in cultivation are shown.
32892290	5	54	theme	new	543:545	arg1	concept					552:558	this new food concept	538:558	this new food concept	538:558	However, still many aspects need to be resolved before this new food concept can enter the market.
32892290	2	55	from	Applicability	146:158	arg1	cultivation					238:248	cultivation	238:248	cultivation	238:248	Applicability of dairy side streams as carbon source and use of natural growth enhancers in cultivation are shown.
32892290	4	56	theme	food	466:469	arg1	production					471:480	current food production	458:480	current food production	458:480	Plant cell cultures used for food could supplement current food production.
32892290	5	57	theme	food	547:550	arg1	concept					552:558	this new food concept	538:558	this new food concept	538:558	However, still many aspects need to be resolved before this new food concept can enter the market.
32892290	1	58	theme	cell	109:112	arg1	cultures					114:121	plant cell cultures	103:121	plant cell cultures	103:121	Sustainability and safety aspects of plant cell cultures as food are presented.
32892290	0	59	theme	sustainability	39:52	arg1	food-aspects					23:34	food-aspects	23:34	food-aspects of sustainability and safety	23:63	Plant cell cultures as food-aspects of sustainability and safety.
32892290	7	60	theme	arctic	750:755	arg1	bramble					757:763	arctic bramble	750:763	arctic bramble (Rubus arcticus)	750:780	In this study, two plant cell cultures, deriving from arctic bramble (Rubus arcticus) and birch (Betula pendula), were cultivated using lactose-rich dairy side streams as alternative carbon sources to replace sucrose.
32892290	1	61	theme	cultures	114:121	arg1	aspects					92:98	Sustainability and safety aspects	66:98	Sustainability and safety aspects of plant cell cultures as food	66:129	Sustainability and safety aspects of plant cell cultures as food are presented.
32892290	7	62	theme	carbon	879:884	arg1	streams					856:862	lactose-rich dairy side streams	832:862	lactose-rich dairy side streams	832:862	In this study, two plant cell cultures, deriving from arctic bramble (Rubus arcticus) and birch (Betula pendula), were cultivated using lactose-rich dairy side streams as alternative carbon sources to replace sucrose.
32892290	7	62	theme	carbon	879:884	arg1	sources					886:892	alternative carbon sources	867:892	alternative carbon sources	867:892	In this study, two plant cell cultures, deriving from arctic bramble (Rubus arcticus) and birch (Betula pendula), were cultivated using lactose-rich dairy side streams as alternative carbon sources to replace sucrose.
32892290	11	63	theme	crustaceans	1489:1499	arg1	assay					1501:1505	freshwater crustaceans assay	1478:1505	freshwater crustaceans assay	1478:1505	Finally, it was shown that only trace amounts of free growth regulators are present in the cells at the harvesting point and assessment by freshwater crustaceans assay indicated that toxicity of the cells was not exceeding that of traditionally consumed bilberry fruit.
32892290	3	64	theme	cellular	289:296	arg1	products					298:305	Biotechnologically produced cellular products	261:305	Biotechnologically produced cellular products	261:305	Biotechnologically produced cellular products are currently emerging to replace and add into the portfolio of agriculturally derived commodities.
32892290	10	65	dep	coconut	1286:1292	arg1	water					1294:1298	water	1294:1298	water	1294:1298	Synthetic plant growth regulators were replaced by natural components, such as coconut water and IAA for several subculture cycles.
32892290	2	66	theme	carbon	185:190	arg1	source					192:197	carbon source	185:197	carbon source	185:197	Applicability of dairy side streams as carbon source and use of natural growth enhancers in cultivation are shown.
32892290	0	67	dep	cultures	11:18	arg1	food-aspects					23:34	food-aspects	23:34	food-aspects of sustainability and safety	23:63	Plant cell cultures as food-aspects of sustainability and safety.
32892290	10	68	theme	several	1312:1318	arg1	cycles					1331:1336	several subculture cycles	1312:1336	several subculture cycles	1312:1336	Synthetic plant growth regulators were replaced by natural components, such as coconut water and IAA for several subculture cycles.
32892290	11	69	theme	regulators	1400:1409	arg1	amounts					1377:1383	only trace amounts	1366:1383	only trace amounts of free growth regulators	1366:1409	Finally, it was shown that only trace amounts of free growth regulators are present in the cells at the harvesting point and assessment by freshwater crustaceans assay indicated that toxicity of the cells was not exceeding that of traditionally consumed bilberry fruit.
32892290	11	69	theme	regulators	1400:1409	arg1	regulators					1400:1409	free growth regulators	1388:1409	free growth regulators	1388:1409	Finally, it was shown that only trace amounts of free growth regulators are present in the cells at the harvesting point and assessment by freshwater crustaceans assay indicated that toxicity of the cells was not exceeding that of traditionally consumed bilberry fruit.
32892290	11	70	from	present	1415:1421	arg1	cells					1430:1434	the cells	1426:1434	the cells	1426:1434	Finally, it was shown that only trace amounts of free growth regulators are present in the cells at the harvesting point and assessment by freshwater crustaceans assay indicated that toxicity of the cells was not exceeding that of traditionally consumed bilberry fruit.
32892290	3	71	link	derived	386:392	arg1	commodities					394:404	agriculturally derived commodities	371:404	agriculturally derived commodities	371:404	Biotechnologically produced cellular products are currently emerging to replace and add into the portfolio of agriculturally derived commodities.
32892290	7	72	theme	side	851:854	arg1	streams					856:862	lactose-rich dairy side streams	832:862	lactose-rich dairy side streams	832:862	In this study, two plant cell cultures, deriving from arctic bramble (Rubus arcticus) and birch (Betula pendula), were cultivated using lactose-rich dairy side streams as alternative carbon sources to replace sucrose.
32892290	7	72	theme	side	851:854	arg1	sources					886:892	alternative carbon sources	867:892	alternative carbon sources	867:892	In this study, two plant cell cultures, deriving from arctic bramble (Rubus arcticus) and birch (Betula pendula), were cultivated using lactose-rich dairy side streams as alternative carbon sources to replace sucrose.
32892290	11	73	from	point	1454:1458	arg1	present					1415:1421	present	1415:1421	present	1415:1421	Finally, it was shown that only trace amounts of free growth regulators are present in the cells at the harvesting point and assessment by freshwater crustaceans assay indicated that toxicity of the cells was not exceeding that of traditionally consumed bilberry fruit.
32892290	7	74	theme	alternative	867:877	arg1	streams					856:862	lactose-rich dairy side streams	832:862	lactose-rich dairy side streams	832:862	In this study, two plant cell cultures, deriving from arctic bramble (Rubus arcticus) and birch (Betula pendula), were cultivated using lactose-rich dairy side streams as alternative carbon sources to replace sucrose.
32892290	7	74	theme	alternative	867:877	arg1	sources					886:892	alternative carbon sources	867:892	alternative carbon sources	867:892	In this study, two plant cell cultures, deriving from arctic bramble (Rubus arcticus) and birch (Betula pendula), were cultivated using lactose-rich dairy side streams as alternative carbon sources to replace sucrose.
32892290	0	75	theme	safety	58:63	arg1	food-aspects					23:34	food-aspects	23:34	food-aspects of sustainability and safety	23:63	Plant cell cultures as food-aspects of sustainability and safety.
32892290	9	76	theme	sensory	1166:1172	arg1	properties					1174:1183	sensory properties	1166:1183	sensory properties	1166:1183	Furthermore, nutritional composition or sensory properties were not compromised.
32892290	2	77	theme	enhancers	225:233	arg1	use					203:205	use	203:205	use of natural growth enhancers in cultivation	203:248	Applicability of dairy side streams as carbon source and use of natural growth enhancers in cultivation are shown.
32892290	2	77	theme	enhancers	225:233	arg1	Applicability					146:158	Applicability	146:158	Applicability of dairy side streams as carbon source	146:197	Applicability of dairy side streams as carbon source and use of natural growth enhancers in cultivation are shown.
32892290	11	78	theme	cells	1538:1542	arg1	toxicity					1522:1529	toxicity	1522:1529	toxicity of the cells	1522:1542	Finally, it was shown that only trace amounts of free growth regulators are present in the cells at the harvesting point and assessment by freshwater crustaceans assay indicated that toxicity of the cells was not exceeding that of traditionally consumed bilberry fruit.
32892290	3	79	theme	derived	386:392	arg1	commodities					394:404	agriculturally derived commodities	371:404	agriculturally derived commodities	371:404	Biotechnologically produced cellular products are currently emerging to replace and add into the portfolio of agriculturally derived commodities.
32892290	6	80	theme	human	630:634	arg1	consumption					636:646	human consumption	630:646	human consumption	630:646	Issues related to sustainability and safety for human consumption are relevant for both consumers and regulators.
31830181	2	0	theme	palladium	345:353	arg1	nanoparticles					355:367	palladium nanoparticles	345:367	palladium nanoparticles	345:367	Here, we develop a facile approach for in situ growth of palladium nanoparticles in an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1).
31830181	4	1	used	used	807:810	arg2	transducer					824:833	a signal transducer	815:833	a signal transducer	815:833	Based on these features, the designed nanocomposite was used as a signal transducer to develop a colorimetric assay and multicolor imaging for accurate and sensitive detection of cancer cells.
31830181	4	1	used	used	807:810	arg2	nanocomposite					789:801	the designed nanocomposite	776:801	the designed nanocomposite	776:801	Based on these features, the designed nanocomposite was used as a signal transducer to develop a colorimetric assay and multicolor imaging for accurate and sensitive detection of cancer cells.
31830181	1	2	theme	early	261:265	arg1	diagnosis					267:275	early diagnosis	261:275	early diagnosis	261:275	Accurate, sensitive detection of cancer cells from clinical fluids is helpful for screening and early diagnosis of tumors.
31830181	7	3	theme	COF-based	1221:1229	arg1	Pd					1252:1253	Pd NPs/CMC-COF-LZU1	1252:1270	Pd NPs/CMC-COF-LZU1	1252:1270	We envision that these COF-based composite materials (Pd NPs/CMC-COF-LZU1) have tremendous potential applications in biotechnology and biological sciences.
31830181	7	3	theme	COF-based	1221:1229	arg1	materials					1241:1249	these COF-based composite materials	1215:1249	these COF-based composite materials (Pd NPs/CMC-COF-LZU1)	1215:1271	We envision that these COF-based composite materials (Pd NPs/CMC-COF-LZU1) have tremendous potential applications in biotechnology and biological sciences.
31830181	5	4	theme	detection	992:1000	arg1	system					1002:1007	the detection system	988:1007	the detection system	988:1007	The transformation of NNPH into NPH enabled the detection system to perform multicolor imaging of HeLa cells.
31830181	6	5	theme	%	1191:1191	arg1	FBS					1193:1195	10% FBS	1189:1195	10% FBS	1189:1195	By using folic acid (FA) as a recognition element, a total of 100 cancer cells (HeLa) can be distinguished in 1 mL culture medium with 10% FBS.
31830181	4	6	theme	cells	937:941	arg1	detection					917:925	accurate and sensitive detection	894:925	accurate and sensitive detection of cancer cells	894:941	Based on these features, the designed nanocomposite was used as a signal transducer to develop a colorimetric assay and multicolor imaging for accurate and sensitive detection of cancer cells.
31830181	6	7	theme	1	1164:1164	arg1	mL					1166:1167	mL	1166:1167	mL	1166:1167	By using folic acid (FA) as a recognition element, a total of 100 cancer cells (HeLa) can be distinguished in 1 mL culture medium with 10% FBS.
31830181	0	8	theme	colorimetric	102:113	arg1	detection					115:123	colorimetric detection	102:123	colorimetric detection	102:123	Aqueous stable Pd nanoparticles/covalent organic framework nanocomposite: an efficient nanoenzyme for colorimetric detection and multicolor imaging of cancer cells.
31830181	4	9	theme	cancer	930:935	arg1	cells					937:941	cancer cells	930:941	cancer cells	930:941	Based on these features, the designed nanocomposite was used as a signal transducer to develop a colorimetric assay and multicolor imaging for accurate and sensitive detection of cancer cells.
31830181	2	10	from	growth	335:340	arg1	hydrogel					450:457	an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel	372:457	an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1)	372:485	Here, we develop a facile approach for in situ growth of palladium nanoparticles in an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1).
31830181	2	10	from	growth	335:340	arg1	NPs/CMC-COF-LZU1					469:484	named Pd NPs/CMC-COF-LZU1	460:484	named Pd NPs/CMC-COF-LZU1	460:484	Here, we develop a facile approach for in situ growth of palladium nanoparticles in an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1).
31830181	5	11	theme	multicolor	1020:1029	arg1	imaging					1031:1037	multicolor imaging	1020:1037	multicolor imaging of HeLa cells	1020:1051	The transformation of NNPH into NPH enabled the detection system to perform multicolor imaging of HeLa cells.
31830181	1	12	theme	tumors	280:285	arg1	screening					247:255	screening	247:255	screening	247:255	Accurate, sensitive detection of cancer cells from clinical fluids is helpful for screening and early diagnosis of tumors.
31830181	1	12	theme	tumors	280:285	arg1	diagnosis					267:275	early diagnosis	261:275	early diagnosis	261:275	Accurate, sensitive detection of cancer cells from clinical fluids is helpful for screening and early diagnosis of tumors.
31830181	0	13	theme	multicolor	129:138	arg1	imaging					140:146	multicolor imaging	129:146	multicolor imaging	129:146	Aqueous stable Pd nanoparticles/covalent organic framework nanocomposite: an efficient nanoenzyme for colorimetric detection and multicolor imaging of cancer cells.
31830181	7	14	theme	composite	1231:1239	arg1	Pd					1252:1253	Pd NPs/CMC-COF-LZU1	1252:1270	Pd NPs/CMC-COF-LZU1	1252:1270	We envision that these COF-based composite materials (Pd NPs/CMC-COF-LZU1) have tremendous potential applications in biotechnology and biological sciences.
31830181	7	14	theme	composite	1231:1239	arg1	materials					1241:1249	these COF-based composite materials	1215:1249	these COF-based composite materials (Pd NPs/CMC-COF-LZU1)	1215:1271	We envision that these COF-based composite materials (Pd NPs/CMC-COF-LZU1) have tremendous potential applications in biotechnology and biological sciences.
31830181	4	15	theme	accurate	894:901	arg1	detection					917:925	accurate and sensitive detection	894:925	accurate and sensitive detection of cancer cells	894:941	Based on these features, the designed nanocomposite was used as a signal transducer to develop a colorimetric assay and multicolor imaging for accurate and sensitive detection of cancer cells.
31830181	2	16	theme	organic	432:438	arg1	hydrogel					450:457	an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel	372:457	an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1)	372:485	Here, we develop a facile approach for in situ growth of palladium nanoparticles in an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1).
31830181	2	16	theme	organic	432:438	arg1	NPs/CMC-COF-LZU1					469:484	named Pd NPs/CMC-COF-LZU1	460:484	named Pd NPs/CMC-COF-LZU1	460:484	Here, we develop a facile approach for in situ growth of palladium nanoparticles in an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1).
31830181	3	17	theme	catalytic	549:557	arg1	performance					559:569	superior catalytic performance	540:569	superior catalytic performance	540:569	The resulting nanocomposite has been proven to show superior catalytic performance for the transformation of N-butyl-4-NHAlloc-1,8-naphthalimide (NNPH) into N-butyl-4-amido-1,8-naphthalimide (NPH), indicated by significant changes in both color and fluorescence.
31830181	2	18	theme	aqueous	375:381	arg1	hydrogel					450:457	an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel	372:457	an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1)	372:485	Here, we develop a facile approach for in situ growth of palladium nanoparticles in an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1).
31830181	2	18	theme	aqueous	375:381	arg1	NPs/CMC-COF-LZU1					469:484	named Pd NPs/CMC-COF-LZU1	460:484	named Pd NPs/CMC-COF-LZU1	460:484	Here, we develop a facile approach for in situ growth of palladium nanoparticles in an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1).
31830181	2	19	theme	carboxymethyl	390:402	arg1	hydrogel					450:457	an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel	372:457	an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1)	372:485	Here, we develop a facile approach for in situ growth of palladium nanoparticles in an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1).
31830181	2	19	theme	carboxymethyl	390:402	arg1	NPs/CMC-COF-LZU1					469:484	named Pd NPs/CMC-COF-LZU1	460:484	named Pd NPs/CMC-COF-LZU1	460:484	Here, we develop a facile approach for in situ growth of palladium nanoparticles in an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1).
31830181	3	20	from	changes	711:717	arg1	fluorescence					737:748	fluorescence	737:748	fluorescence	737:748	The resulting nanocomposite has been proven to show superior catalytic performance for the transformation of N-butyl-4-NHAlloc-1,8-naphthalimide (NNPH) into N-butyl-4-amido-1,8-naphthalimide (NPH), indicated by significant changes in both color and fluorescence.
31830181	3	20	from	changes	711:717	arg1	color					727:731	color	727:731	color	727:731	The resulting nanocomposite has been proven to show superior catalytic performance for the transformation of N-butyl-4-NHAlloc-1,8-naphthalimide (NNPH) into N-butyl-4-amido-1,8-naphthalimide (NPH), indicated by significant changes in both color and fluorescence.
31830181	2	21	theme	Pd	466:467	arg1	hydrogel					450:457	an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel	372:457	an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1)	372:485	Here, we develop a facile approach for in situ growth of palladium nanoparticles in an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1).
31830181	2	21	theme	Pd	466:467	arg1	NPs/CMC-COF-LZU1					469:484	named Pd NPs/CMC-COF-LZU1	460:484	named Pd NPs/CMC-COF-LZU1	460:484	Here, we develop a facile approach for in situ growth of palladium nanoparticles in an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1).
31830181	2	22	theme	stable	383:388	arg1	hydrogel					450:457	an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel	372:457	an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1)	372:485	Here, we develop a facile approach for in situ growth of palladium nanoparticles in an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1).
31830181	2	22	theme	stable	383:388	arg1	NPs/CMC-COF-LZU1					469:484	named Pd NPs/CMC-COF-LZU1	460:484	named Pd NPs/CMC-COF-LZU1	460:484	Here, we develop a facile approach for in situ growth of palladium nanoparticles in an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1).
31830181	0	23	theme	stable	8:13	arg1	nanocomposite					59:71	Aqueous stable Pd nanoparticles/covalent organic framework nanocomposite	0:71	Aqueous stable Pd nanoparticles/covalent organic framework nanocomposite: an efficient nanoenzyme for colorimetric detection and multicolor imaging of cancer cells.	0:163	Aqueous stable Pd nanoparticles/covalent organic framework nanocomposite: an efficient nanoenzyme for colorimetric detection and multicolor imaging of cancer cells.
31830181	3	24	theme	superior	540:547	arg1	performance					559:569	superior catalytic performance	540:569	superior catalytic performance	540:569	The resulting nanocomposite has been proven to show superior catalytic performance for the transformation of N-butyl-4-NHAlloc-1,8-naphthalimide (NNPH) into N-butyl-4-amido-1,8-naphthalimide (NPH), indicated by significant changes in both color and fluorescence.
31830181	4	25	theme	sensitive	907:915	arg1	detection					917:925	accurate and sensitive detection	894:925	accurate and sensitive detection of cancer cells	894:941	Based on these features, the designed nanocomposite was used as a signal transducer to develop a colorimetric assay and multicolor imaging for accurate and sensitive detection of cancer cells.
31830181	2	26	dep	in	327:328	arg1	situ					330:333	situ	330:333	situ	330:333	Here, we develop a facile approach for in situ growth of palladium nanoparticles in an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1).
31830181	1	27	theme	cancer	198:203	arg1	cells					205:209	cancer cells	198:209	cancer cells	198:209	Accurate, sensitive detection of cancer cells from clinical fluids is helpful for screening and early diagnosis of tumors.
31830181	0	28	theme	Aqueous	0:6	arg1	nanocomposite					59:71	Aqueous stable Pd nanoparticles/covalent organic framework nanocomposite	0:71	Aqueous stable Pd nanoparticles/covalent organic framework nanocomposite: an efficient nanoenzyme for colorimetric detection and multicolor imaging of cancer cells.	0:163	Aqueous stable Pd nanoparticles/covalent organic framework nanocomposite: an efficient nanoenzyme for colorimetric detection and multicolor imaging of cancer cells.
31830181	6	29	theme	10	1189:1190	arg1	%					1191:1191	%	1191:1191	%	1191:1191	By using folic acid (FA) as a recognition element, a total of 100 cancer cells (HeLa) can be distinguished in 1 mL culture medium with 10% FBS.
31830181	2	30	theme	facile	307:312	arg1	approach					314:321	a facile approach	305:321	a facile approach for in situ growth of palladium nanoparticles in an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1)	305:485	Here, we develop a facile approach for in situ growth of palladium nanoparticles in an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1).
31830181	1	31	theme	cells	205:209	arg1	detection					185:193	Accurate, sensitive detection	165:193	Accurate, sensitive detection of cancer cells from clinical fluids	165:230	Accurate, sensitive detection of cancer cells from clinical fluids is helpful for screening and early diagnosis of tumors.
31830181	0	32	theme	nanoparticles/covalent	18:39	arg1	nanocomposite					59:71	Aqueous stable Pd nanoparticles/covalent organic framework nanocomposite	0:71	Aqueous stable Pd nanoparticles/covalent organic framework nanocomposite: an efficient nanoenzyme for colorimetric detection and multicolor imaging of cancer cells.	0:163	Aqueous stable Pd nanoparticles/covalent organic framework nanocomposite: an efficient nanoenzyme for colorimetric detection and multicolor imaging of cancer cells.
31830181	4	33	theme	designed	780:787	arg1	nanocomposite					789:801	the designed nanocomposite	776:801	the designed nanocomposite	776:801	Based on these features, the designed nanocomposite was used as a signal transducer to develop a colorimetric assay and multicolor imaging for accurate and sensitive detection of cancer cells.
31830181	4	33	theme	designed	780:787	arg1	transducer					824:833	a signal transducer	815:833	a signal transducer	815:833	Based on these features, the designed nanocomposite was used as a signal transducer to develop a colorimetric assay and multicolor imaging for accurate and sensitive detection of cancer cells.
31830181	2	34	theme	framework	440:448	arg1	hydrogel					450:457	an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel	372:457	an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1)	372:485	Here, we develop a facile approach for in situ growth of palladium nanoparticles in an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1).
31830181	2	34	theme	framework	440:448	arg1	NPs/CMC-COF-LZU1					469:484	named Pd NPs/CMC-COF-LZU1	460:484	named Pd NPs/CMC-COF-LZU1	460:484	Here, we develop a facile approach for in situ growth of palladium nanoparticles in an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1).
31830181	0	35	theme	Pd	15:16	arg1	nanocomposite					59:71	Aqueous stable Pd nanoparticles/covalent organic framework nanocomposite	0:71	Aqueous stable Pd nanoparticles/covalent organic framework nanocomposite: an efficient nanoenzyme for colorimetric detection and multicolor imaging of cancer cells.	0:163	Aqueous stable Pd nanoparticles/covalent organic framework nanocomposite: an efficient nanoenzyme for colorimetric detection and multicolor imaging of cancer cells.
31830181	6	36	with	medium	1177:1182	arg1	FBS					1193:1195	10% FBS	1189:1195	10% FBS	1189:1195	By using folic acid (FA) as a recognition element, a total of 100 cancer cells (HeLa) can be distinguished in 1 mL culture medium with 10% FBS.
31830181	2	37	theme	named	460:464	arg1	hydrogel					450:457	an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel	372:457	an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1)	372:485	Here, we develop a facile approach for in situ growth of palladium nanoparticles in an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1).
31830181	2	37	theme	named	460:464	arg1	NPs/CMC-COF-LZU1					469:484	named Pd NPs/CMC-COF-LZU1	460:484	named Pd NPs/CMC-COF-LZU1	460:484	Here, we develop a facile approach for in situ growth of palladium nanoparticles in an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1).
31830181	3	38	theme	significant	699:709	arg1	changes					711:717	significant changes	699:717	significant changes in both color and fluorescence	699:748	The resulting nanocomposite has been proven to show superior catalytic performance for the transformation of N-butyl-4-NHAlloc-1,8-naphthalimide (NNPH) into N-butyl-4-amido-1,8-naphthalimide (NPH), indicated by significant changes in both color and fluorescence.
31830181	1	39	dep	Accurate	165:172	arg1	sensitive					175:183	sensitive	175:183	sensitive	175:183	Accurate, sensitive detection of cancer cells from clinical fluids is helpful for screening and early diagnosis of tumors.
31830181	1	40	theme	clinical	216:223	arg1	fluids					225:230	clinical fluids	216:230	clinical fluids	216:230	Accurate, sensitive detection of cancer cells from clinical fluids is helpful for screening and early diagnosis of tumors.
31830181	0	41	theme	framework	49:57	arg1	nanocomposite					59:71	Aqueous stable Pd nanoparticles/covalent organic framework nanocomposite	0:71	Aqueous stable Pd nanoparticles/covalent organic framework nanocomposite: an efficient nanoenzyme for colorimetric detection and multicolor imaging of cancer cells.	0:163	Aqueous stable Pd nanoparticles/covalent organic framework nanocomposite: an efficient nanoenzyme for colorimetric detection and multicolor imaging of cancer cells.
31830181	0	42	theme	cells	158:162	arg1	detection					115:123	colorimetric detection	102:123	colorimetric detection	102:123	Aqueous stable Pd nanoparticles/covalent organic framework nanocomposite: an efficient nanoenzyme for colorimetric detection and multicolor imaging of cancer cells.
31830181	0	42	theme	cells	158:162	arg1	imaging					140:146	multicolor imaging	129:146	multicolor imaging	129:146	Aqueous stable Pd nanoparticles/covalent organic framework nanocomposite: an efficient nanoenzyme for colorimetric detection and multicolor imaging of cancer cells.
31830181	7	43	contain	have	1273:1276	arg1	Pd					1252:1253	Pd NPs/CMC-COF-LZU1	1252:1270	Pd NPs/CMC-COF-LZU1	1252:1270	We envision that these COF-based composite materials (Pd NPs/CMC-COF-LZU1) have tremendous potential applications in biotechnology and biological sciences.
31830181	7	43	contain	have	1273:1276	arg1	materials					1241:1249	these COF-based composite materials	1215:1249	these COF-based composite materials (Pd NPs/CMC-COF-LZU1)	1215:1271	We envision that these COF-based composite materials (Pd NPs/CMC-COF-LZU1) have tremendous potential applications in biotechnology and biological sciences.
31830181	7	43	contain	have	1273:1276	arg2	applications					1299:1310	tremendous potential applications	1278:1310	tremendous potential applications	1278:1310	We envision that these COF-based composite materials (Pd NPs/CMC-COF-LZU1) have tremendous potential applications in biotechnology and biological sciences.
31830181	6	44	theme	recognition	1084:1094	arg1	element					1096:1102	a recognition element	1082:1102	a recognition element	1082:1102	By using folic acid (FA) as a recognition element, a total of 100 cancer cells (HeLa) can be distinguished in 1 mL culture medium with 10% FBS.
31830181	6	44	theme	recognition	1084:1094	arg1	acid					1069:1072	folic acid	1063:1072	folic acid (FA)	1063:1077	By using folic acid (FA) as a recognition element, a total of 100 cancer cells (HeLa) can be distinguished in 1 mL culture medium with 10% FBS.
31830181	2	45	theme	covalent	423:430	arg1	hydrogel					450:457	an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel	372:457	an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1)	372:485	Here, we develop a facile approach for in situ growth of palladium nanoparticles in an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1).
31830181	2	45	theme	covalent	423:430	arg1	NPs/CMC-COF-LZU1					469:484	named Pd NPs/CMC-COF-LZU1	460:484	named Pd NPs/CMC-COF-LZU1	460:484	Here, we develop a facile approach for in situ growth of palladium nanoparticles in an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1).
31830181	0	46	theme	organic	41:47	arg1	nanocomposite					59:71	Aqueous stable Pd nanoparticles/covalent organic framework nanocomposite	0:71	Aqueous stable Pd nanoparticles/covalent organic framework nanocomposite: an efficient nanoenzyme for colorimetric detection and multicolor imaging of cancer cells.	0:163	Aqueous stable Pd nanoparticles/covalent organic framework nanocomposite: an efficient nanoenzyme for colorimetric detection and multicolor imaging of cancer cells.
31830181	0	47	theme	cancer	151:156	arg1	cells					158:162	cancer cells	151:162	cancer cells	151:162	Aqueous stable Pd nanoparticles/covalent organic framework nanocomposite: an efficient nanoenzyme for colorimetric detection and multicolor imaging of cancer cells.
31830181	0	48	dep	nanocomposite	59:71	arg1	nanoenzyme					87:96	an efficient nanoenzyme	74:96	Aqueous stable Pd nanoparticles/covalent organic framework nanocomposite: an efficient nanoenzyme for colorimetric detection and multicolor imaging of cancer cells.	0:163	Aqueous stable Pd nanoparticles/covalent organic framework nanocomposite: an efficient nanoenzyme for colorimetric detection and multicolor imaging of cancer cells.
31830181	1	49	from	fluids	225:230	arg1	detection					185:193	Accurate, sensitive detection	165:193	Accurate, sensitive detection of cancer cells from clinical fluids	165:230	Accurate, sensitive detection of cancer cells from clinical fluids is helpful for screening and early diagnosis of tumors.
31830181	2	50	theme	cellulose-modified	404:421	arg1	hydrogel					450:457	an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel	372:457	an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1)	372:485	Here, we develop a facile approach for in situ growth of palladium nanoparticles in an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1).
31830181	2	50	theme	cellulose-modified	404:421	arg1	NPs/CMC-COF-LZU1					469:484	named Pd NPs/CMC-COF-LZU1	460:484	named Pd NPs/CMC-COF-LZU1	460:484	Here, we develop a facile approach for in situ growth of palladium nanoparticles in an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1).
31830181	5	51	theme	NNPH	966:969	arg1	transformation					948:961	The transformation	944:961	The transformation of NNPH into NPH	944:978	The transformation of NNPH into NPH enabled the detection system to perform multicolor imaging of HeLa cells.
31830181	6	52	theme	culture	1169:1175	arg1	medium					1177:1182	1 mL culture medium	1164:1182	1 mL culture medium with 10% FBS	1164:1195	By using folic acid (FA) as a recognition element, a total of 100 cancer cells (HeLa) can be distinguished in 1 mL culture medium with 10% FBS.
31830181	4	53	theme	multicolor	871:880	arg1	imaging					882:888	multicolor imaging	871:888	multicolor imaging	871:888	Based on these features, the designed nanocomposite was used as a signal transducer to develop a colorimetric assay and multicolor imaging for accurate and sensitive detection of cancer cells.
31830181	7	54	theme	biological	1333:1342	arg1	sciences					1344:1351	biological sciences	1333:1351	biological sciences	1333:1351	We envision that these COF-based composite materials (Pd NPs/CMC-COF-LZU1) have tremendous potential applications in biotechnology and biological sciences.
31830181	3	55	theme	N-butyl-4-NHAlloc-1,8-naphthalimide	597:631	arg1	transformation					579:592	the transformation	575:592	the transformation of N-butyl-4-NHAlloc-1,8-naphthalimide (NNPH) into N-butyl-4-amido-1,8-naphthalimide (NPH), indicated by significant changes in both color and fluorescence	575:748	The resulting nanocomposite has been proven to show superior catalytic performance for the transformation of N-butyl-4-NHAlloc-1,8-naphthalimide (NNPH) into N-butyl-4-amido-1,8-naphthalimide (NPH), indicated by significant changes in both color and fluorescence.
31830181	5	56	theme	HeLa	1042:1045	arg1	cells					1047:1051	HeLa cells	1042:1051	HeLa cells	1042:1051	The transformation of NNPH into NPH enabled the detection system to perform multicolor imaging of HeLa cells.
31830181	2	57	theme	in	327:328	arg1	growth					335:340	in situ growth	327:340	in situ growth of palladium nanoparticles in an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1)	327:485	Here, we develop a facile approach for in situ growth of palladium nanoparticles in an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1).
31830181	6	58	theme	cells	1127:1131	arg1	total					1107:1111	a total	1105:1111	a total of 100 cancer cells (HeLa)	1105:1138	By using folic acid (FA) as a recognition element, a total of 100 cancer cells (HeLa) can be distinguished in 1 mL culture medium with 10% FBS.
31830181	4	59	theme	signal	817:822	arg1	nanocomposite					789:801	the designed nanocomposite	776:801	the designed nanocomposite	776:801	Based on these features, the designed nanocomposite was used as a signal transducer to develop a colorimetric assay and multicolor imaging for accurate and sensitive detection of cancer cells.
31830181	4	59	theme	signal	817:822	arg1	transducer					824:833	a signal transducer	815:833	a signal transducer	815:833	Based on these features, the designed nanocomposite was used as a signal transducer to develop a colorimetric assay and multicolor imaging for accurate and sensitive detection of cancer cells.
31830181	5	60	theme	cells	1047:1051	arg1	imaging					1031:1037	multicolor imaging	1020:1037	multicolor imaging of HeLa cells	1020:1051	The transformation of NNPH into NPH enabled the detection system to perform multicolor imaging of HeLa cells.
31830181	2	61	theme	nanoparticles	355:367	arg1	growth					335:340	in situ growth	327:340	in situ growth of palladium nanoparticles in an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1)	327:485	Here, we develop a facile approach for in situ growth of palladium nanoparticles in an aqueous stable carboxymethyl cellulose-modified covalent organic framework hydrogel (named Pd NPs/CMC-COF-LZU1).
31830181	3	62	theme	resulting	492:500	arg1	nanocomposite					502:514	The resulting nanocomposite	488:514	The resulting nanocomposite	488:514	The resulting nanocomposite has been proven to show superior catalytic performance for the transformation of N-butyl-4-NHAlloc-1,8-naphthalimide (NNPH) into N-butyl-4-amido-1,8-naphthalimide (NPH), indicated by significant changes in both color and fluorescence.
31830181	0	63	theme	efficient	77:85	arg1	nanoenzyme					87:96	an efficient nanoenzyme	74:96	Aqueous stable Pd nanoparticles/covalent organic framework nanocomposite: an efficient nanoenzyme for colorimetric detection and multicolor imaging of cancer cells.	0:163	Aqueous stable Pd nanoparticles/covalent organic framework nanocomposite: an efficient nanoenzyme for colorimetric detection and multicolor imaging of cancer cells.
31830181	7	64	theme	tremendous	1278:1287	arg1	applications					1299:1310	tremendous potential applications	1278:1310	tremendous potential applications	1278:1310	We envision that these COF-based composite materials (Pd NPs/CMC-COF-LZU1) have tremendous potential applications in biotechnology and biological sciences.
31830181	4	65	theme	colorimetric	848:859	arg1	assay					861:865	a colorimetric assay	846:865	a colorimetric assay	846:865	Based on these features, the designed nanocomposite was used as a signal transducer to develop a colorimetric assay and multicolor imaging for accurate and sensitive detection of cancer cells.
31830181	7	66	theme	potential	1289:1297	arg1	applications					1299:1310	tremendous potential applications	1278:1310	tremendous potential applications	1278:1310	We envision that these COF-based composite materials (Pd NPs/CMC-COF-LZU1) have tremendous potential applications in biotechnology and biological sciences.
31830181	6	67	theme	cancer	1120:1125	arg1	HeLa					1134:1137	HeLa	1134:1137	HeLa	1134:1137	By using folic acid (FA) as a recognition element, a total of 100 cancer cells (HeLa) can be distinguished in 1 mL culture medium with 10% FBS.
31830181	6	67	theme	cancer	1120:1125	arg1	cells					1127:1131	100 cancer cells	1116:1131	100 cancer cells (HeLa)	1116:1138	By using folic acid (FA) as a recognition element, a total of 100 cancer cells (HeLa) can be distinguished in 1 mL culture medium with 10% FBS.
31830181	6	68	theme	mL	1166:1167	arg1	medium					1177:1182	1 mL culture medium	1164:1182	1 mL culture medium with 10% FBS	1164:1195	By using folic acid (FA) as a recognition element, a total of 100 cancer cells (HeLa) can be distinguished in 1 mL culture medium with 10% FBS.
31830181	6	69	theme	folic	1063:1067	arg1	element					1096:1102	a recognition element	1082:1102	a recognition element	1082:1102	By using folic acid (FA) as a recognition element, a total of 100 cancer cells (HeLa) can be distinguished in 1 mL culture medium with 10% FBS.
31830181	6	69	theme	folic	1063:1067	arg1	FA					1075:1076	FA	1075:1076	FA	1075:1076	By using folic acid (FA) as a recognition element, a total of 100 cancer cells (HeLa) can be distinguished in 1 mL culture medium with 10% FBS.
31830181	6	69	theme	folic	1063:1067	arg1	acid					1069:1072	folic acid	1063:1072	folic acid (FA)	1063:1077	By using folic acid (FA) as a recognition element, a total of 100 cancer cells (HeLa) can be distinguished in 1 mL culture medium with 10% FBS.
31830181	1	70	theme	Accurate	165:172	arg1	detection					185:193	Accurate, sensitive detection	165:193	Accurate, sensitive detection of cancer cells from clinical fluids	165:230	Accurate, sensitive detection of cancer cells from clinical fluids is helpful for screening and early diagnosis of tumors.
33125241	5	0	theme	short	848:852	arg1	glycopeptides					873:885	short aliphatic O-linked glycopeptides	848:885	short aliphatic O-linked glycopeptides	848:885	Using three Burkholderia species (Burkholderia cenocepacia, Burkholderia Dolosa, and Burkholderia ubonensis), we demonstrate that short aliphatic O-linked glycopeptides are typically absent from HILIC enrichments, yet are readily identified in whole proteome samples.
33125241	6	1	theme	ion	1023:1025	arg1	FAIMS					1050:1054	FAIMS	1050:1054	FAIMS	1050:1054	Using high-field asymmetric waveform ion mobility spectrometry (FAIMS) fractionation, we show that at high compensation voltages (CVs), short aliphatic glycopeptides can be enriched from complex samples, providing an alternative means to identify glycopeptide recalcitrant to hydrophilic-based enrichment.
33125241	6	1	theme	ion	1023:1025	arg1	spectrometry					1036:1047	asymmetric waveform ion mobility spectrometry	1003:1047	high-field asymmetric waveform ion mobility spectrometry (FAIMS) fractionation	992:1069	Using high-field asymmetric waveform ion mobility spectrometry (FAIMS) fractionation, we show that at high compensation voltages (CVs), short aliphatic glycopeptides can be enriched from complex samples, providing an alternative means to identify glycopeptide recalcitrant to hydrophilic-based enrichment.
33125241	2	2	gly	glycopeptide	191:202	arg2	glycopeptide					191:202	Hydrophilic interaction liquid chromatography (HILIC) glycopeptide enrichment	137:213	Hydrophilic interaction liquid chromatography (HILIC) glycopeptide enrichment	137:213	Hydrophilic interaction liquid chromatography (HILIC) glycopeptide enrichment is an indispensable tool for the high-throughput characterization of glycoproteomes.
33125241	4	3	theme	HILIC	635:639	arg1	approaches					664:673	HILIC enrichment-independent approaches	635:673	HILIC enrichment-independent approaches for the study of bacterial glycoproteomes	635:715	Here, we investigate HILIC enrichment-independent approaches for the study of bacterial glycoproteomes.
33125241	5	4	theme	aliphatic	854:862	arg1	glycopeptides					873:885	short aliphatic O-linked glycopeptides	848:885	short aliphatic O-linked glycopeptides	848:885	Using three Burkholderia species (Burkholderia cenocepacia, Burkholderia Dolosa, and Burkholderia ubonensis), we demonstrate that short aliphatic O-linked glycopeptides are typically absent from HILIC enrichments, yet are readily identified in whole proteome samples.
33125241	6	5	theme	waveform	1014:1021	arg1	FAIMS					1050:1054	FAIMS	1050:1054	FAIMS	1050:1054	Using high-field asymmetric waveform ion mobility spectrometry (FAIMS) fractionation, we show that at high compensation voltages (CVs), short aliphatic glycopeptides can be enriched from complex samples, providing an alternative means to identify glycopeptide recalcitrant to hydrophilic-based enrichment.
33125241	6	5	theme	waveform	1014:1021	arg1	spectrometry					1036:1047	asymmetric waveform ion mobility spectrometry	1003:1047	high-field asymmetric waveform ion mobility spectrometry (FAIMS) fractionation	992:1069	Using high-field asymmetric waveform ion mobility spectrometry (FAIMS) fractionation, we show that at high compensation voltages (CVs), short aliphatic glycopeptides can be enriched from complex samples, providing an alternative means to identify glycopeptide recalcitrant to hydrophilic-based enrichment.
33125241	5	6	theme	Burkholderia	730:741	arg1	species					743:749	three Burkholderia species	724:749	three Burkholderia species (Burkholderia cenocepacia, Burkholderia Dolosa, and Burkholderia ubonensis)	724:825	Using three Burkholderia species (Burkholderia cenocepacia, Burkholderia Dolosa, and Burkholderia ubonensis), we demonstrate that short aliphatic O-linked glycopeptides are typically absent from HILIC enrichments, yet are readily identified in whole proteome samples.
33125241	6	7	theme	complex	1173:1179	arg1	samples					1181:1187	complex samples	1173:1187	complex samples	1173:1187	Using high-field asymmetric waveform ion mobility spectrometry (FAIMS) fractionation, we show that at high compensation voltages (CVs), short aliphatic glycopeptides can be enriched from complex samples, providing an alternative means to identify glycopeptide recalcitrant to hydrophilic-based enrichment.
33125241	6	8	theme	high	1088:1091	arg1	CVs					1116:1118	CVs	1116:1118	CVs	1116:1118	Using high-field asymmetric waveform ion mobility spectrometry (FAIMS) fractionation, we show that at high compensation voltages (CVs), short aliphatic glycopeptides can be enriched from complex samples, providing an alternative means to identify glycopeptide recalcitrant to hydrophilic-based enrichment.
33125241	6	8	theme	high	1088:1091	arg1	voltages					1106:1113	high compensation voltages	1088:1113	high compensation voltages (CVs)	1088:1119	Using high-field asymmetric waveform ion mobility spectrometry (FAIMS) fractionation, we show that at high compensation voltages (CVs), short aliphatic glycopeptides can be enriched from complex samples, providing an alternative means to identify glycopeptide recalcitrant to hydrophilic-based enrichment.
33125241	3	9	used	used	547:550	arg2	concentrations					513:526	high concentrations	508:526	high concentrations of formic acid	508:541	Despite its utility, HILIC enrichment is associated with a number of shortcomings, including requiring large amounts of starting materials, potentially introducing chemical artifacts such as formylation when high concentrations of formic acid are used, and biasing/undersampling specific classes of glycopeptides.
33125241	6	10	theme	high-field	992:1001	arg1	fractionation					1057:1069	high-field asymmetric waveform ion mobility spectrometry (FAIMS) fractionation	992:1069	high-field asymmetric waveform ion mobility spectrometry (FAIMS) fractionation	992:1069	Using high-field asymmetric waveform ion mobility spectrometry (FAIMS) fractionation, we show that at high compensation voltages (CVs), short aliphatic glycopeptides can be enriched from complex samples, providing an alternative means to identify glycopeptide recalcitrant to hydrophilic-based enrichment.
33125241	9	11	from	CVs	1659:1661	arg1	enrichable					1634:1643	enrichable	1634:1643	enrichable	1634:1643	fetus also being enrichable at high FAIMS CVs.
33125241	2	12	theme	chromatography	168:181	arg1	tool					235:238	an indispensable tool	218:238	an indispensable tool for the high-throughput characterization of glycoproteomes	218:297	Hydrophilic interaction liquid chromatography (HILIC) glycopeptide enrichment is an indispensable tool for the high-throughput characterization of glycoproteomes.
33125241	2	12	theme	chromatography	168:181	arg1	enrichment					204:213	Hydrophilic interaction liquid chromatography (HILIC) glycopeptide enrichment	137:213	Hydrophilic interaction liquid chromatography (HILIC) glycopeptide enrichment	137:213	Hydrophilic interaction liquid chromatography (HILIC) glycopeptide enrichment is an indispensable tool for the high-throughput characterization of glycoproteomes.
33125241	3	13	theme	glycopeptides	599:611	arg1	classes					588:594	specific classes	579:594	specific classes of glycopeptides	579:611	Despite its utility, HILIC enrichment is associated with a number of shortcomings, including requiring large amounts of starting materials, potentially introducing chemical artifacts such as formylation when high concentrations of formic acid are used, and biasing/undersampling specific classes of glycopeptides.
33125241	4	14	theme	enrichment-independent	641:662	arg1	approaches					664:673	HILIC enrichment-independent approaches	635:673	HILIC enrichment-independent approaches for the study of bacterial glycoproteomes	635:715	Here, we investigate HILIC enrichment-independent approaches for the study of bacterial glycoproteomes.
33125241	3	15	theme	shortcomings	369:380	arg1	number					359:364	a number	357:364	a number of shortcomings	357:380	Despite its utility, HILIC enrichment is associated with a number of shortcomings, including requiring large amounts of starting materials, potentially introducing chemical artifacts such as formylation when high concentrations of formic acid are used, and biasing/undersampling specific classes of glycopeptides.
33125241	2	16	theme	liquid	161:166	arg1	HILIC					184:188	HILIC	184:188	HILIC	184:188	Hydrophilic interaction liquid chromatography (HILIC) glycopeptide enrichment is an indispensable tool for the high-throughput characterization of glycoproteomes.
33125241	2	16	theme	liquid	161:166	arg1	chromatography					168:181	Hydrophilic interaction liquid chromatography	137:181	Hydrophilic interaction liquid chromatography (HILIC) glycopeptide enrichment	137:213	Hydrophilic interaction liquid chromatography (HILIC) glycopeptide enrichment is an indispensable tool for the high-throughput characterization of glycoproteomes.
33125241	6	17	theme	aliphatic	1128:1136	arg1	glycopeptides					1138:1150	short aliphatic glycopeptides	1122:1150	short aliphatic glycopeptides	1122:1150	Using high-field asymmetric waveform ion mobility spectrometry (FAIMS) fractionation, we show that at high compensation voltages (CVs), short aliphatic glycopeptides can be enriched from complex samples, providing an alternative means to identify glycopeptide recalcitrant to hydrophilic-based enrichment.
33125241	1	18	theme	Bacterial	63:71	arg1	Coverage					87:94	Bacterial Glycoproteome Coverage	63:94	Bacterial Glycoproteome Coverage	63:94	Improving Bacterial Glycoproteome Coverage Using Total Proteome and FAIMS Analyses.
33125241	5	19	theme	whole	962:966	arg1	samples					977:983	whole proteome samples	962:983	whole proteome samples	962:983	Using three Burkholderia species (Burkholderia cenocepacia, Burkholderia Dolosa, and Burkholderia ubonensis), we demonstrate that short aliphatic O-linked glycopeptides are typically absent from HILIC enrichments, yet are readily identified in whole proteome samples.
33125241	8	20	link	N-linked	1564:1571	arg1	glycopeptides					1573:1585	the N-linked glycopeptides	1560:1585	the N-linked glycopeptides of Campylobacter fetus subsp	1560:1614	Excitingly, the ability to enrich glycopeptides using FAIMS appears generally applicable, with the N-linked glycopeptides of Campylobacter fetus subsp.
33125241	2	21	theme	interaction	149:159	arg1	HILIC					184:188	HILIC	184:188	HILIC	184:188	Hydrophilic interaction liquid chromatography (HILIC) glycopeptide enrichment is an indispensable tool for the high-throughput characterization of glycoproteomes.
33125241	2	21	theme	interaction	149:159	arg1	chromatography					168:181	Hydrophilic interaction liquid chromatography	137:181	Hydrophilic interaction liquid chromatography (HILIC) glycopeptide enrichment	137:213	Hydrophilic interaction liquid chromatography (HILIC) glycopeptide enrichment is an indispensable tool for the high-throughput characterization of glycoproteomes.
33125241	6	22	theme	compensation	1093:1104	arg1	CVs					1116:1118	CVs	1116:1118	CVs	1116:1118	Using high-field asymmetric waveform ion mobility spectrometry (FAIMS) fractionation, we show that at high compensation voltages (CVs), short aliphatic glycopeptides can be enriched from complex samples, providing an alternative means to identify glycopeptide recalcitrant to hydrophilic-based enrichment.
33125241	6	22	theme	compensation	1093:1104	arg1	voltages					1106:1113	high compensation voltages	1088:1113	high compensation voltages (CVs)	1088:1119	Using high-field asymmetric waveform ion mobility spectrometry (FAIMS) fractionation, we show that at high compensation voltages (CVs), short aliphatic glycopeptides can be enriched from complex samples, providing an alternative means to identify glycopeptide recalcitrant to hydrophilic-based enrichment.
33125241	1	23	theme	Glycoproteome	73:85	arg1	Coverage					87:94	Bacterial Glycoproteome Coverage	63:94	Bacterial Glycoproteome Coverage	63:94	Improving Bacterial Glycoproteome Coverage Using Total Proteome and FAIMS Analyses.
33125241	7	24	theme	whole	1302:1306	arg1	proteome					1308:1315	whole proteome	1302:1315	whole proteome	1302:1315	Combining whole proteome and FAIMS analyses, we show that the observable glycoproteome of these Burkholderia species is at least 25% larger than what was initially thought.
33125241	5	25	dep	species	743:749	arg1	ubonensis					816:824	Burkholderia ubonensis	803:824	Burkholderia ubonensis	803:824	Using three Burkholderia species (Burkholderia cenocepacia, Burkholderia Dolosa, and Burkholderia ubonensis), we demonstrate that short aliphatic O-linked glycopeptides are typically absent from HILIC enrichments, yet are readily identified in whole proteome samples.
33125241	5	25	dep	species	743:749	arg1	cenocepacia					765:775	Burkholderia cenocepacia	752:775	Burkholderia cenocepacia	752:775	Using three Burkholderia species (Burkholderia cenocepacia, Burkholderia Dolosa, and Burkholderia ubonensis), we demonstrate that short aliphatic O-linked glycopeptides are typically absent from HILIC enrichments, yet are readily identified in whole proteome samples.
33125241	5	25	dep	species	743:749	arg1	Dolosa					791:796	Burkholderia Dolosa	778:796	Burkholderia Dolosa	778:796	Using three Burkholderia species (Burkholderia cenocepacia, Burkholderia Dolosa, and Burkholderia ubonensis), we demonstrate that short aliphatic O-linked glycopeptides are typically absent from HILIC enrichments, yet are readily identified in whole proteome samples.
33125241	2	26	theme	Hydrophilic	137:147	arg1	HILIC					184:188	HILIC	184:188	HILIC	184:188	Hydrophilic interaction liquid chromatography (HILIC) glycopeptide enrichment is an indispensable tool for the high-throughput characterization of glycoproteomes.
33125241	2	26	theme	Hydrophilic	137:147	arg1	chromatography					168:181	Hydrophilic interaction liquid chromatography	137:181	Hydrophilic interaction liquid chromatography (HILIC) glycopeptide enrichment	137:213	Hydrophilic interaction liquid chromatography (HILIC) glycopeptide enrichment is an indispensable tool for the high-throughput characterization of glycoproteomes.
33125241	5	27	from	enrichments	919:929	arg1	absent					901:906	absent	901:906	absent	901:906	Using three Burkholderia species (Burkholderia cenocepacia, Burkholderia Dolosa, and Burkholderia ubonensis), we demonstrate that short aliphatic O-linked glycopeptides are typically absent from HILIC enrichments, yet are readily identified in whole proteome samples.
33125241	3	28	theme	chemical	464:471	arg1	artifacts					473:481	chemical artifacts	464:481	chemical artifacts such as formylation when high concentrations of formic acid are used	464:550	Despite its utility, HILIC enrichment is associated with a number of shortcomings, including requiring large amounts of starting materials, potentially introducing chemical artifacts such as formylation when high concentrations of formic acid are used, and biasing/undersampling specific classes of glycopeptides.
33125241	3	28	theme	chemical	464:471	arg1	formylation					491:501	formylation	491:501	formylation when high concentrations of formic acid are used	491:550	Despite its utility, HILIC enrichment is associated with a number of shortcomings, including requiring large amounts of starting materials, potentially introducing chemical artifacts such as formylation when high concentrations of formic acid are used, and biasing/undersampling specific classes of glycopeptides.
33125241	3	29	theme	starting	420:427	arg1	materials					429:437	starting materials	420:437	starting materials	420:437	Despite its utility, HILIC enrichment is associated with a number of shortcomings, including requiring large amounts of starting materials, potentially introducing chemical artifacts such as formylation when high concentrations of formic acid are used, and biasing/undersampling specific classes of glycopeptides.
33125241	10	30	gly	glycopeptides	1757:1769	arg2	glycopeptides					1757:1769	access glycopeptides	1750:1769	access glycopeptides	1750:1769	Taken together, these results demonstrate that FAIMS provides an alternative means to access glycopeptides and is a valuable tool for glycoproteomic analysis.
33125241	10	31	theme	glycoproteomic	1798:1811	arg1	analysis					1813:1820	glycoproteomic analysis	1798:1820	glycoproteomic analysis	1798:1820	Taken together, these results demonstrate that FAIMS provides an alternative means to access glycopeptides and is a valuable tool for glycoproteomic analysis.
33125241	5	32	theme	proteome	968:975	arg1	samples					977:983	whole proteome samples	962:983	whole proteome samples	962:983	Using three Burkholderia species (Burkholderia cenocepacia, Burkholderia Dolosa, and Burkholderia ubonensis), we demonstrate that short aliphatic O-linked glycopeptides are typically absent from HILIC enrichments, yet are readily identified in whole proteome samples.
33125241	3	33	theme	large	403:407	arg1	amounts					409:415	large amounts	403:415	large amounts of starting materials	403:437	Despite its utility, HILIC enrichment is associated with a number of shortcomings, including requiring large amounts of starting materials, potentially introducing chemical artifacts such as formylation when high concentrations of formic acid are used, and biasing/undersampling specific classes of glycopeptides.
33125241	3	33	theme	large	403:407	arg1	materials					429:437	starting materials	420:437	starting materials	420:437	Despite its utility, HILIC enrichment is associated with a number of shortcomings, including requiring large amounts of starting materials, potentially introducing chemical artifacts such as formylation when high concentrations of formic acid are used, and biasing/undersampling specific classes of glycopeptides.
33125241	1	34	theme	Total	102:106	arg1	Proteome					108:115	Total Proteome	102:115	Total Proteome	102:115	Improving Bacterial Glycoproteome Coverage Using Total Proteome and FAIMS Analyses.
33125241	3	35	theme	high	508:511	arg1	concentrations					513:526	high concentrations	508:526	high concentrations of formic acid	508:541	Despite its utility, HILIC enrichment is associated with a number of shortcomings, including requiring large amounts of starting materials, potentially introducing chemical artifacts such as formylation when high concentrations of formic acid are used, and biasing/undersampling specific classes of glycopeptides.
33125241	6	36	gly	glycopeptide	1233:1244	arg2	glycopeptide					1233:1244	glycopeptide	1233:1244	glycopeptide	1233:1244	Using high-field asymmetric waveform ion mobility spectrometry (FAIMS) fractionation, we show that at high compensation voltages (CVs), short aliphatic glycopeptides can be enriched from complex samples, providing an alternative means to identify glycopeptide recalcitrant to hydrophilic-based enrichment.
33125241	3	37	theme	specific	579:586	arg1	classes					588:594	specific classes	579:594	specific classes of glycopeptides	579:611	Despite its utility, HILIC enrichment is associated with a number of shortcomings, including requiring large amounts of starting materials, potentially introducing chemical artifacts such as formylation when high concentrations of formic acid are used, and biasing/undersampling specific classes of glycopeptides.
33125241	2	38	theme	glycopeptide	191:202	arg1	tool					235:238	an indispensable tool	218:238	an indispensable tool for the high-throughput characterization of glycoproteomes	218:297	Hydrophilic interaction liquid chromatography (HILIC) glycopeptide enrichment is an indispensable tool for the high-throughput characterization of glycoproteomes.
33125241	2	38	theme	glycopeptide	191:202	arg1	enrichment					204:213	Hydrophilic interaction liquid chromatography (HILIC) glycopeptide enrichment	137:213	Hydrophilic interaction liquid chromatography (HILIC) glycopeptide enrichment	137:213	Hydrophilic interaction liquid chromatography (HILIC) glycopeptide enrichment is an indispensable tool for the high-throughput characterization of glycoproteomes.
33125241	9	39	theme	high	1648:1651	arg1	CVs					1659:1661	high FAIMS CVs	1648:1661	high FAIMS CVs	1648:1661	fetus also being enrichable at high FAIMS CVs.
33125241	4	40	theme	bacterial	692:700	arg1	glycoproteomes					702:715	bacterial glycoproteomes	692:715	bacterial glycoproteomes	692:715	Here, we investigate HILIC enrichment-independent approaches for the study of bacterial glycoproteomes.
33125241	5	41	theme	O-linked	864:871	arg1	glycopeptides					873:885	short aliphatic O-linked glycopeptides	848:885	short aliphatic O-linked glycopeptides	848:885	Using three Burkholderia species (Burkholderia cenocepacia, Burkholderia Dolosa, and Burkholderia ubonensis), we demonstrate that short aliphatic O-linked glycopeptides are typically absent from HILIC enrichments, yet are readily identified in whole proteome samples.
33125241	7	42	theme	observable	1354:1363	arg1	larger					1425:1430	larger	1425:1430	larger	1425:1430	Combining whole proteome and FAIMS analyses, we show that the observable glycoproteome of these Burkholderia species is at least 25% larger than what was initially thought.
33125241	7	42	theme	observable	1354:1363	arg1	glycoproteome					1365:1377	the observable glycoproteome	1350:1377	the observable glycoproteome of these Burkholderia species	1350:1407	Combining whole proteome and FAIMS analyses, we show that the observable glycoproteome of these Burkholderia species is at least 25% larger than what was initially thought.
33125241	2	43	theme	high-throughput	248:262	arg1	characterization					264:279	the high-throughput characterization	244:279	the high-throughput characterization of glycoproteomes	244:297	Hydrophilic interaction liquid chromatography (HILIC) glycopeptide enrichment is an indispensable tool for the high-throughput characterization of glycoproteomes.
33125241	10	44	theme	access	1750:1755	arg1	glycopeptides					1757:1769	access glycopeptides	1750:1769	access glycopeptides	1750:1769	Taken together, these results demonstrate that FAIMS provides an alternative means to access glycopeptides and is a valuable tool for glycoproteomic analysis.
33125241	6	45	theme	short	1122:1126	arg1	glycopeptides					1138:1150	short aliphatic glycopeptides	1122:1150	short aliphatic glycopeptides	1122:1150	Using high-field asymmetric waveform ion mobility spectrometry (FAIMS) fractionation, we show that at high compensation voltages (CVs), short aliphatic glycopeptides can be enriched from complex samples, providing an alternative means to identify glycopeptide recalcitrant to hydrophilic-based enrichment.
33125241	8	46	gly	glycopeptides	1499:1511	arg2	glycopeptides					1499:1511	glycopeptides	1499:1511	glycopeptides using FAIMS	1499:1523	Excitingly, the ability to enrich glycopeptides using FAIMS appears generally applicable, with the N-linked glycopeptides of Campylobacter fetus subsp.
33125241	8	47	theme	N-linked	1564:1571	arg1	glycopeptides					1573:1585	the N-linked glycopeptides	1560:1585	the N-linked glycopeptides of Campylobacter fetus subsp	1560:1614	Excitingly, the ability to enrich glycopeptides using FAIMS appears generally applicable, with the N-linked glycopeptides of Campylobacter fetus subsp.
33125241	5	48	theme	HILIC	913:917	arg1	enrichments					919:929	HILIC enrichments	913:929	HILIC enrichments	913:929	Using three Burkholderia species (Burkholderia cenocepacia, Burkholderia Dolosa, and Burkholderia ubonensis), we demonstrate that short aliphatic O-linked glycopeptides are typically absent from HILIC enrichments, yet are readily identified in whole proteome samples.
33125241	8	49	gly	glycopeptides	1573:1585	arg1	subsp					1610:1614	Campylobacter fetus subsp	1590:1614	Campylobacter fetus subsp	1590:1614	Excitingly, the ability to enrich glycopeptides using FAIMS appears generally applicable, with the N-linked glycopeptides of Campylobacter fetus subsp.
33125241	8	49	gly	glycopeptides	1573:1585	arg2	glycopeptides					1573:1585	the N-linked glycopeptides	1560:1585	the N-linked glycopeptides of Campylobacter fetus subsp	1560:1614	Excitingly, the ability to enrich glycopeptides using FAIMS appears generally applicable, with the N-linked glycopeptides of Campylobacter fetus subsp.
33125241	3	50	theme	materials	429:437	arg1	amounts					409:415	large amounts	403:415	large amounts of starting materials	403:437	Despite its utility, HILIC enrichment is associated with a number of shortcomings, including requiring large amounts of starting materials, potentially introducing chemical artifacts such as formylation when high concentrations of formic acid are used, and biasing/undersampling specific classes of glycopeptides.
33125241	3	50	theme	materials	429:437	arg1	materials					429:437	starting materials	420:437	starting materials	420:437	Despite its utility, HILIC enrichment is associated with a number of shortcomings, including requiring large amounts of starting materials, potentially introducing chemical artifacts such as formylation when high concentrations of formic acid are used, and biasing/undersampling specific classes of glycopeptides.
33125241	5	51	link	O-linked	864:871	arg1	glycopeptides					873:885	short aliphatic O-linked glycopeptides	848:885	short aliphatic O-linked glycopeptides	848:885	Using three Burkholderia species (Burkholderia cenocepacia, Burkholderia Dolosa, and Burkholderia ubonensis), we demonstrate that short aliphatic O-linked glycopeptides are typically absent from HILIC enrichments, yet are readily identified in whole proteome samples.
33125241	3	52	theme	acid	538:541	arg1	concentrations					513:526	high concentrations	508:526	high concentrations of formic acid	508:541	Despite its utility, HILIC enrichment is associated with a number of shortcomings, including requiring large amounts of starting materials, potentially introducing chemical artifacts such as formylation when high concentrations of formic acid are used, and biasing/undersampling specific classes of glycopeptides.
33125241	0	53	theme	Hydrophilic	29:39	arg1	Enrichment					41:50	Hydrophilic Enrichment	29:50	Hydrophilic Enrichment	29:50	What Are We Missing by Using Hydrophilic Enrichment?
33125241	7	54	dep	proteome	1308:1315	arg1	analyses					1327:1334	analyses	1327:1334	analyses	1327:1334	Combining whole proteome and FAIMS analyses, we show that the observable glycoproteome of these Burkholderia species is at least 25% larger than what was initially thought.
33125241	2	55	theme	indispensable	221:233	arg1	tool					235:238	an indispensable tool	218:238	an indispensable tool for the high-throughput characterization of glycoproteomes	218:297	Hydrophilic interaction liquid chromatography (HILIC) glycopeptide enrichment is an indispensable tool for the high-throughput characterization of glycoproteomes.
33125241	2	55	theme	indispensable	221:233	arg1	enrichment					204:213	Hydrophilic interaction liquid chromatography (HILIC) glycopeptide enrichment	137:213	Hydrophilic interaction liquid chromatography (HILIC) glycopeptide enrichment	137:213	Hydrophilic interaction liquid chromatography (HILIC) glycopeptide enrichment is an indispensable tool for the high-throughput characterization of glycoproteomes.
33125241	7	56	theme	Burkholderia	1388:1399	arg1	species					1401:1407	these Burkholderia species	1382:1407	these Burkholderia species	1382:1407	Combining whole proteome and FAIMS analyses, we show that the observable glycoproteome of these Burkholderia species is at least 25% larger than what was initially thought.
33125241	7	57	theme	species	1401:1407	arg1	larger					1425:1430	larger	1425:1430	larger	1425:1430	Combining whole proteome and FAIMS analyses, we show that the observable glycoproteome of these Burkholderia species is at least 25% larger than what was initially thought.
33125241	7	57	theme	species	1401:1407	arg1	glycoproteome					1365:1377	the observable glycoproteome	1350:1377	the observable glycoproteome of these Burkholderia species	1350:1407	Combining whole proteome and FAIMS analyses, we show that the observable glycoproteome of these Burkholderia species is at least 25% larger than what was initially thought.
33125241	6	58	gly	glycopeptides	1138:1150	arg2	glycopeptides					1138:1150	short aliphatic glycopeptides	1122:1150	short aliphatic glycopeptides	1122:1150	Using high-field asymmetric waveform ion mobility spectrometry (FAIMS) fractionation, we show that at high compensation voltages (CVs), short aliphatic glycopeptides can be enriched from complex samples, providing an alternative means to identify glycopeptide recalcitrant to hydrophilic-based enrichment.
33125241	6	59	theme	spectrometry	1036:1047	arg1	fractionation					1057:1069	high-field asymmetric waveform ion mobility spectrometry (FAIMS) fractionation	992:1069	high-field asymmetric waveform ion mobility spectrometry (FAIMS) fractionation	992:1069	Using high-field asymmetric waveform ion mobility spectrometry (FAIMS) fractionation, we show that at high compensation voltages (CVs), short aliphatic glycopeptides can be enriched from complex samples, providing an alternative means to identify glycopeptide recalcitrant to hydrophilic-based enrichment.
33125241	3	60	theme	HILIC	321:325	arg1	enrichment					327:336	HILIC enrichment	321:336	HILIC enrichment	321:336	Despite its utility, HILIC enrichment is associated with a number of shortcomings, including requiring large amounts of starting materials, potentially introducing chemical artifacts such as formylation when high concentrations of formic acid are used, and biasing/undersampling specific classes of glycopeptides.
33125241	5	61	gly	glycopeptides	873:885	arg2	glycopeptides					873:885	short aliphatic O-linked glycopeptides	848:885	short aliphatic O-linked glycopeptides	848:885	Using three Burkholderia species (Burkholderia cenocepacia, Burkholderia Dolosa, and Burkholderia ubonensis), we demonstrate that short aliphatic O-linked glycopeptides are typically absent from HILIC enrichments, yet are readily identified in whole proteome samples.
33125241	10	62	theme	valuable	1780:1787	arg1	tool					1789:1792	a valuable tool	1778:1792	a valuable tool for glycoproteomic analysis	1778:1820	Taken together, these results demonstrate that FAIMS provides an alternative means to access glycopeptides and is a valuable tool for glycoproteomic analysis.
33125241	6	63	theme	alternative	1203:1213	arg1	means					1215:1219	an alternative means	1200:1219	an alternative means to identify glycopeptide recalcitrant to hydrophilic-based enrichment	1200:1289	Using high-field asymmetric waveform ion mobility spectrometry (FAIMS) fractionation, we show that at high compensation voltages (CVs), short aliphatic glycopeptides can be enriched from complex samples, providing an alternative means to identify glycopeptide recalcitrant to hydrophilic-based enrichment.
33125241	9	64	theme	FAIMS	1653:1657	arg1	CVs					1659:1661	high FAIMS CVs	1648:1661	high FAIMS CVs	1648:1661	fetus also being enrichable at high FAIMS CVs.
33125241	6	65	theme	hydrophilic-based	1262:1278	arg1	enrichment					1280:1289	hydrophilic-based enrichment	1262:1289	hydrophilic-based enrichment	1262:1289	Using high-field asymmetric waveform ion mobility spectrometry (FAIMS) fractionation, we show that at high compensation voltages (CVs), short aliphatic glycopeptides can be enriched from complex samples, providing an alternative means to identify glycopeptide recalcitrant to hydrophilic-based enrichment.
33125241	8	66	theme	fetus	1604:1608	arg1	subsp					1610:1614	Campylobacter fetus subsp	1590:1614	Campylobacter fetus subsp	1590:1614	Excitingly, the ability to enrich glycopeptides using FAIMS appears generally applicable, with the N-linked glycopeptides of Campylobacter fetus subsp.
33125241	2	67	theme	glycoproteomes	284:297	arg1	characterization					264:279	the high-throughput characterization	244:279	the high-throughput characterization of glycoproteomes	244:297	Hydrophilic interaction liquid chromatography (HILIC) glycopeptide enrichment is an indispensable tool for the high-throughput characterization of glycoproteomes.
33125241	8	68	theme	subsp	1610:1614	arg1	glycopeptides					1573:1585	the N-linked glycopeptides	1560:1585	the N-linked glycopeptides of Campylobacter fetus subsp	1560:1614	Excitingly, the ability to enrich glycopeptides using FAIMS appears generally applicable, with the N-linked glycopeptides of Campylobacter fetus subsp.
33125241	3	69	theme	formic	531:536	arg1	acid					538:541	formic acid	531:541	formic acid	531:541	Despite its utility, HILIC enrichment is associated with a number of shortcomings, including requiring large amounts of starting materials, potentially introducing chemical artifacts such as formylation when high concentrations of formic acid are used, and biasing/undersampling specific classes of glycopeptides.
33125241	8	70	theme	Campylobacter	1590:1602	arg1	subsp					1610:1614	Campylobacter fetus subsp	1590:1614	Campylobacter fetus subsp	1590:1614	Excitingly, the ability to enrich glycopeptides using FAIMS appears generally applicable, with the N-linked glycopeptides of Campylobacter fetus subsp.
33125241	4	71	theme	glycoproteomes	702:715	arg1	study					683:687	the study	679:687	the study of bacterial glycoproteomes	679:715	Here, we investigate HILIC enrichment-independent approaches for the study of bacterial glycoproteomes.
33125241	3	72	gly	glycopeptides	599:611	arg2	glycopeptides					599:611	glycopeptides	599:611	glycopeptides	599:611	Despite its utility, HILIC enrichment is associated with a number of shortcomings, including requiring large amounts of starting materials, potentially introducing chemical artifacts such as formylation when high concentrations of formic acid are used, and biasing/undersampling specific classes of glycopeptides.
33125241	6	73	theme	asymmetric	1003:1012	arg1	FAIMS					1050:1054	FAIMS	1050:1054	FAIMS	1050:1054	Using high-field asymmetric waveform ion mobility spectrometry (FAIMS) fractionation, we show that at high compensation voltages (CVs), short aliphatic glycopeptides can be enriched from complex samples, providing an alternative means to identify glycopeptide recalcitrant to hydrophilic-based enrichment.
33125241	6	73	theme	asymmetric	1003:1012	arg1	spectrometry					1036:1047	asymmetric waveform ion mobility spectrometry	1003:1047	high-field asymmetric waveform ion mobility spectrometry (FAIMS) fractionation	992:1069	Using high-field asymmetric waveform ion mobility spectrometry (FAIMS) fractionation, we show that at high compensation voltages (CVs), short aliphatic glycopeptides can be enriched from complex samples, providing an alternative means to identify glycopeptide recalcitrant to hydrophilic-based enrichment.
33125241	10	74	theme	alternative	1729:1739	arg1	means					1741:1745	an alternative means	1726:1745	an alternative means to access glycopeptides	1726:1769	Taken together, these results demonstrate that FAIMS provides an alternative means to access glycopeptides and is a valuable tool for glycoproteomic analysis.
33125241	6	75	theme	mobility	1027:1034	arg1	FAIMS					1050:1054	FAIMS	1050:1054	FAIMS	1050:1054	Using high-field asymmetric waveform ion mobility spectrometry (FAIMS) fractionation, we show that at high compensation voltages (CVs), short aliphatic glycopeptides can be enriched from complex samples, providing an alternative means to identify glycopeptide recalcitrant to hydrophilic-based enrichment.
33125241	6	75	theme	mobility	1027:1034	arg1	spectrometry					1036:1047	asymmetric waveform ion mobility spectrometry	1003:1047	high-field asymmetric waveform ion mobility spectrometry (FAIMS) fractionation	992:1069	Using high-field asymmetric waveform ion mobility spectrometry (FAIMS) fractionation, we show that at high compensation voltages (CVs), short aliphatic glycopeptides can be enriched from complex samples, providing an alternative means to identify glycopeptide recalcitrant to hydrophilic-based enrichment.
33125241	1	76	dep	Proteome	108:115	arg1	Analyses					127:134	Analyses	127:134	Analyses	127:134	Improving Bacterial Glycoproteome Coverage Using Total Proteome and FAIMS Analyses.
34747049	5	0	from	colitis	1039:1045	arg1	mice					1050:1053	mice	1050:1053	mice	1050:1053	SNX10 deletion or treatment with DC-SX029, a novel SNX10 inhibitor, rescues OMV-induced intestinal barrier dysfunction and ameliorates colitis in mice by blocking cytosolic LPS release, caspase-5 activation, and downstream signaling.
34747049	0	1	theme	signaling	91:99	arg1	cascade					101:107	a caspase-5-dependent signaling cascade	69:107	a caspase-5-dependent signaling cascade	69:107	SNX10-mediated LPS sensing causes intestinal barrier dysfunction via a caspase-5-dependent signaling cascade.
34747049	4	2	theme	cytosolic	719:727	arg1	LPS					729:731	cytosolic LPS	719:731	cytosolic LPS	719:731	Caspase-5 activated by cytosolic LPS leads to Lyn phosphorylation, which in turn promotes nuclear translocalization of Snail/Slug, downregulation of E-cadherin expression, and intestinal barrier dysfunction.
34747049	4	3	theme	Snail/Slug	815:824	arg1	downregulation					827:840	downregulation	827:840	downregulation of E-cadherin expression	827:865	Caspase-5 activated by cytosolic LPS leads to Lyn phosphorylation, which in turn promotes nuclear translocalization of Snail/Slug, downregulation of E-cadherin expression, and intestinal barrier dysfunction.
34747049	4	3	theme	Snail/Slug	815:824	arg1	dysfunction					891:901	intestinal barrier dysfunction	872:901	intestinal barrier dysfunction	872:901	Caspase-5 activated by cytosolic LPS leads to Lyn phosphorylation, which in turn promotes nuclear translocalization of Snail/Slug, downregulation of E-cadherin expression, and intestinal barrier dysfunction.
34747049	4	3	theme	Snail/Slug	815:824	arg1	translocalization					794:810	nuclear translocalization	786:810	nuclear translocalization of Snail/Slug	786:824	Caspase-5 activated by cytosolic LPS leads to Lyn phosphorylation, which in turn promotes nuclear translocalization of Snail/Slug, downregulation of E-cadherin expression, and intestinal barrier dysfunction.
34747049	5	4	theme	cytosolic	1067:1075	arg1	release					1081:1087	cytosolic LPS release	1067:1087	cytosolic LPS release	1067:1087	SNX10 deletion or treatment with DC-SX029, a novel SNX10 inhibitor, rescues OMV-induced intestinal barrier dysfunction and ameliorates colitis in mice by blocking cytosolic LPS release, caspase-5 activation, and downstream signaling.
34747049	6	5	theme	IBD	1290:1292	arg1	treatment					1294:1302	IBD treatment	1290:1302	IBD treatment	1290:1302	Our results show that targeting SNX10 may be a new therapeutic approach for restoring intestinal epithelial barrier function and promising strategy for IBD treatment.
34747049	3	6	theme	early	587:591	arg1	membranes					603:611	early endosomal membranes	587:611	early endosomal membranes	587:611	Here, we show that the internalization of Gram-negative bacterial outer membrane vesicles (OMVs) in human intestinal epithelial cells promotes recruitment of caspase-5 and PIKfyve to early endosomal membranes via sorting nexin 10 (SNX10), resulting in LPS release from OMVs into the cytosol.
34747049	2	7	theme	inflammation	324:335	arg1	control					313:319	control	313:319	control of inflammation	313:335	Current treatments for IBD are focused on control of inflammation rather than on maintaining intestinal epithelial barrier function.
34747049	6	8	theme	therapeutic	1189:1199	arg1	approach					1201:1208	a new therapeutic approach	1183:1208	a new therapeutic approach for restoring intestinal epithelial barrier function and promising strategy for IBD treatment	1183:1302	Our results show that targeting SNX10 may be a new therapeutic approach for restoring intestinal epithelial barrier function and promising strategy for IBD treatment.
34747049	6	8	theme	therapeutic	1189:1199	arg1	SNX10					1170:1174	targeting SNX10	1160:1174	targeting SNX10	1160:1174	Our results show that targeting SNX10 may be a new therapeutic approach for restoring intestinal epithelial barrier function and promising strategy for IBD treatment.
34747049	6	9	theme	epithelial	1235:1244	arg1	function					1254:1261	intestinal epithelial barrier function	1224:1261	intestinal epithelial barrier function	1224:1261	Our results show that targeting SNX10 may be a new therapeutic approach for restoring intestinal epithelial barrier function and promising strategy for IBD treatment.
34747049	6	10	theme	promising	1267:1275	arg1	strategy					1277:1284	promising strategy	1267:1284	promising strategy for IBD treatment	1267:1302	Our results show that targeting SNX10 may be a new therapeutic approach for restoring intestinal epithelial barrier function and promising strategy for IBD treatment.
34747049	3	11	theme	endosomal	593:601	arg1	membranes					603:611	early endosomal membranes	587:611	early endosomal membranes	587:611	Here, we show that the internalization of Gram-negative bacterial outer membrane vesicles (OMVs) in human intestinal epithelial cells promotes recruitment of caspase-5 and PIKfyve to early endosomal membranes via sorting nexin 10 (SNX10), resulting in LPS release from OMVs into the cytosol.
34747049	3	12	theme	epithelial	521:530	arg1	cells					532:536	human intestinal epithelial cells	504:536	human intestinal epithelial cells	504:536	Here, we show that the internalization of Gram-negative bacterial outer membrane vesicles (OMVs) in human intestinal epithelial cells promotes recruitment of caspase-5 and PIKfyve to early endosomal membranes via sorting nexin 10 (SNX10), resulting in LPS release from OMVs into the cytosol.
34747049	3	13	from	OMVs	673:676	arg1	release					660:666	LPS release	656:666	LPS release from OMVs into the cytosol	656:693	Here, we show that the internalization of Gram-negative bacterial outer membrane vesicles (OMVs) in human intestinal epithelial cells promotes recruitment of caspase-5 and PIKfyve to early endosomal membranes via sorting nexin 10 (SNX10), resulting in LPS release from OMVs into the cytosol.
34747049	5	14	theme	novel	949:953	arg1	deletion					910:917	SNX10 deletion	904:917	SNX10 deletion	904:917	SNX10 deletion or treatment with DC-SX029, a novel SNX10 inhibitor, rescues OMV-induced intestinal barrier dysfunction and ameliorates colitis in mice by blocking cytosolic LPS release, caspase-5 activation, and downstream signaling.
34747049	5	14	theme	novel	949:953	arg1	inhibitor					961:969	a novel SNX10 inhibitor	947:969	a novel SNX10 inhibitor	947:969	SNX10 deletion or treatment with DC-SX029, a novel SNX10 inhibitor, rescues OMV-induced intestinal barrier dysfunction and ameliorates colitis in mice by blocking cytosolic LPS release, caspase-5 activation, and downstream signaling.
34747049	4	15	theme	barrier	883:889	arg1	dysfunction					891:901	intestinal barrier dysfunction	872:901	intestinal barrier dysfunction	872:901	Caspase-5 activated by cytosolic LPS leads to Lyn phosphorylation, which in turn promotes nuclear translocalization of Snail/Slug, downregulation of E-cadherin expression, and intestinal barrier dysfunction.
34747049	3	16	theme	human	504:508	arg1	cells					532:536	human intestinal epithelial cells	504:536	human intestinal epithelial cells	504:536	Here, we show that the internalization of Gram-negative bacterial outer membrane vesicles (OMVs) in human intestinal epithelial cells promotes recruitment of caspase-5 and PIKfyve to early endosomal membranes via sorting nexin 10 (SNX10), resulting in LPS release from OMVs into the cytosol.
34747049	3	17	theme	PIKfyve	576:582	arg1	recruitment					547:557	recruitment	547:557	recruitment of caspase-5 and PIKfyve to early endosomal membranes	547:611	Here, we show that the internalization of Gram-negative bacterial outer membrane vesicles (OMVs) in human intestinal epithelial cells promotes recruitment of caspase-5 and PIKfyve to early endosomal membranes via sorting nexin 10 (SNX10), resulting in LPS release from OMVs into the cytosol.
34747049	3	18	theme	vesicles	485:492	arg1	internalization					427:441	the internalization	423:441	the internalization of Gram-negative bacterial outer membrane vesicles (OMVs) in human intestinal epithelial cells	423:536	Here, we show that the internalization of Gram-negative bacterial outer membrane vesicles (OMVs) in human intestinal epithelial cells promotes recruitment of caspase-5 and PIKfyve to early endosomal membranes via sorting nexin 10 (SNX10), resulting in LPS release from OMVs into the cytosol.
34747049	3	19	theme	Gram-negative	446:458	arg1	vesicles					485:492	Gram-negative bacterial outer membrane vesicles	446:492	Gram-negative bacterial outer membrane vesicles (OMVs)	446:499	Here, we show that the internalization of Gram-negative bacterial outer membrane vesicles (OMVs) in human intestinal epithelial cells promotes recruitment of caspase-5 and PIKfyve to early endosomal membranes via sorting nexin 10 (SNX10), resulting in LPS release from OMVs into the cytosol.
34747049	3	19	theme	Gram-negative	446:458	arg1	OMVs					495:498	OMVs	495:498	OMVs	495:498	Here, we show that the internalization of Gram-negative bacterial outer membrane vesicles (OMVs) in human intestinal epithelial cells promotes recruitment of caspase-5 and PIKfyve to early endosomal membranes via sorting nexin 10 (SNX10), resulting in LPS release from OMVs into the cytosol.
34747049	5	20	theme	barrier	1003:1009	arg1	dysfunction					1011:1021	OMV-induced intestinal barrier dysfunction	980:1021	OMV-induced intestinal barrier dysfunction	980:1021	SNX10 deletion or treatment with DC-SX029, a novel SNX10 inhibitor, rescues OMV-induced intestinal barrier dysfunction and ameliorates colitis in mice by blocking cytosolic LPS release, caspase-5 activation, and downstream signaling.
34747049	0	21	theme	LPS	15:17	arg1	sensing					19:25	SNX10-mediated LPS sensing	0:25	SNX10-mediated LPS sensing	0:25	SNX10-mediated LPS sensing causes intestinal barrier dysfunction via a caspase-5-dependent signaling cascade.
34747049	3	22	theme	sorting	617:623	arg1	SNX10					635:639	SNX10	635:639	SNX10	635:639	Here, we show that the internalization of Gram-negative bacterial outer membrane vesicles (OMVs) in human intestinal epithelial cells promotes recruitment of caspase-5 and PIKfyve to early endosomal membranes via sorting nexin 10 (SNX10), resulting in LPS release from OMVs into the cytosol.
34747049	3	22	theme	sorting	617:623	arg1	nexin					625:629	sorting nexin 10	617:632	sorting nexin 10 (SNX10)	617:640	Here, we show that the internalization of Gram-negative bacterial outer membrane vesicles (OMVs) in human intestinal epithelial cells promotes recruitment of caspase-5 and PIKfyve to early endosomal membranes via sorting nexin 10 (SNX10), resulting in LPS release from OMVs into the cytosol.
34747049	5	23	with	treatment	922:930	arg1	DC-SX029					937:944	DC-SX029	937:944	DC-SX029	937:944	SNX10 deletion or treatment with DC-SX029, a novel SNX10 inhibitor, rescues OMV-induced intestinal barrier dysfunction and ameliorates colitis in mice by blocking cytosolic LPS release, caspase-5 activation, and downstream signaling.
34747049	3	24	theme	outer	470:474	arg1	vesicles					485:492	Gram-negative bacterial outer membrane vesicles	446:492	Gram-negative bacterial outer membrane vesicles (OMVs)	446:499	Here, we show that the internalization of Gram-negative bacterial outer membrane vesicles (OMVs) in human intestinal epithelial cells promotes recruitment of caspase-5 and PIKfyve to early endosomal membranes via sorting nexin 10 (SNX10), resulting in LPS release from OMVs into the cytosol.
34747049	3	24	theme	outer	470:474	arg1	OMVs					495:498	OMVs	495:498	OMVs	495:498	Here, we show that the internalization of Gram-negative bacterial outer membrane vesicles (OMVs) in human intestinal epithelial cells promotes recruitment of caspase-5 and PIKfyve to early endosomal membranes via sorting nexin 10 (SNX10), resulting in LPS release from OMVs into the cytosol.
34747049	0	25	theme	SNX10-mediated	0:13	arg1	sensing					19:25	SNX10-mediated LPS sensing	0:25	SNX10-mediated LPS sensing	0:25	SNX10-mediated LPS sensing causes intestinal barrier dysfunction via a caspase-5-dependent signaling cascade.
34747049	4	26	theme	Lyn	742:744	arg1	phosphorylation					746:760	Lyn phosphorylation	742:760	Lyn phosphorylation	742:760	Caspase-5 activated by cytosolic LPS leads to Lyn phosphorylation, which in turn promotes nuclear translocalization of Snail/Slug, downregulation of E-cadherin expression, and intestinal barrier dysfunction.
34747049	1	27	theme	Altered	110:116	arg1	composition					139:149	Altered intestinal microbial composition	110:149	Altered intestinal microbial composition	110:149	Altered intestinal microbial composition promotes intestinal barrier dysfunction and triggers the initiation and recurrence of inflammatory bowel disease (IBD).
34747049	4	28	theme	nuclear	786:792	arg1	translocalization					794:810	nuclear translocalization	786:810	nuclear translocalization of Snail/Slug	786:824	Caspase-5 activated by cytosolic LPS leads to Lyn phosphorylation, which in turn promotes nuclear translocalization of Snail/Slug, downregulation of E-cadherin expression, and intestinal barrier dysfunction.
34747049	3	29	theme	membrane	476:483	arg1	vesicles					485:492	Gram-negative bacterial outer membrane vesicles	446:492	Gram-negative bacterial outer membrane vesicles (OMVs)	446:499	Here, we show that the internalization of Gram-negative bacterial outer membrane vesicles (OMVs) in human intestinal epithelial cells promotes recruitment of caspase-5 and PIKfyve to early endosomal membranes via sorting nexin 10 (SNX10), resulting in LPS release from OMVs into the cytosol.
34747049	3	29	theme	membrane	476:483	arg1	OMVs					495:498	OMVs	495:498	OMVs	495:498	Here, we show that the internalization of Gram-negative bacterial outer membrane vesicles (OMVs) in human intestinal epithelial cells promotes recruitment of caspase-5 and PIKfyve to early endosomal membranes via sorting nexin 10 (SNX10), resulting in LPS release from OMVs into the cytosol.
34747049	6	30	theme	new	1185:1187	arg1	approach					1201:1208	a new therapeutic approach	1183:1208	a new therapeutic approach for restoring intestinal epithelial barrier function and promising strategy for IBD treatment	1183:1302	Our results show that targeting SNX10 may be a new therapeutic approach for restoring intestinal epithelial barrier function and promising strategy for IBD treatment.
34747049	6	30	theme	new	1185:1187	arg1	SNX10					1170:1174	targeting SNX10	1160:1174	targeting SNX10	1160:1174	Our results show that targeting SNX10 may be a new therapeutic approach for restoring intestinal epithelial barrier function and promising strategy for IBD treatment.
34747049	1	31	theme	intestinal	118:127	arg1	composition					139:149	Altered intestinal microbial composition	110:149	Altered intestinal microbial composition	110:149	Altered intestinal microbial composition promotes intestinal barrier dysfunction and triggers the initiation and recurrence of inflammatory bowel disease (IBD).
34747049	4	32	theme	expression	856:865	arg1	downregulation					827:840	downregulation	827:840	downregulation of E-cadherin expression	827:865	Caspase-5 activated by cytosolic LPS leads to Lyn phosphorylation, which in turn promotes nuclear translocalization of Snail/Slug, downregulation of E-cadherin expression, and intestinal barrier dysfunction.
34747049	4	32	theme	expression	856:865	arg1	dysfunction					891:901	intestinal barrier dysfunction	872:901	intestinal barrier dysfunction	872:901	Caspase-5 activated by cytosolic LPS leads to Lyn phosphorylation, which in turn promotes nuclear translocalization of Snail/Slug, downregulation of E-cadherin expression, and intestinal barrier dysfunction.
34747049	4	32	theme	expression	856:865	arg1	translocalization					794:810	nuclear translocalization	786:810	nuclear translocalization of Snail/Slug	786:824	Caspase-5 activated by cytosolic LPS leads to Lyn phosphorylation, which in turn promotes nuclear translocalization of Snail/Slug, downregulation of E-cadherin expression, and intestinal barrier dysfunction.
34747049	1	33	theme	inflammatory	237:248	arg1	IBD					265:267	IBD	265:267	IBD	265:267	Altered intestinal microbial composition promotes intestinal barrier dysfunction and triggers the initiation and recurrence of inflammatory bowel disease (IBD).
34747049	1	33	theme	inflammatory	237:248	arg1	disease					256:262	inflammatory bowel disease	237:262	inflammatory bowel disease (IBD)	237:268	Altered intestinal microbial composition promotes intestinal barrier dysfunction and triggers the initiation and recurrence of inflammatory bowel disease (IBD).
34747049	5	34	theme	OMV-induced	980:990	arg1	dysfunction					1011:1021	OMV-induced intestinal barrier dysfunction	980:1021	OMV-induced intestinal barrier dysfunction	980:1021	SNX10 deletion or treatment with DC-SX029, a novel SNX10 inhibitor, rescues OMV-induced intestinal barrier dysfunction and ameliorates colitis in mice by blocking cytosolic LPS release, caspase-5 activation, and downstream signaling.
34747049	6	35	theme	barrier	1246:1252	arg1	function					1254:1261	intestinal epithelial barrier function	1224:1261	intestinal epithelial barrier function	1224:1261	Our results show that targeting SNX10 may be a new therapeutic approach for restoring intestinal epithelial barrier function and promising strategy for IBD treatment.
34747049	6	36	theme	intestinal	1224:1233	arg1	function					1254:1261	intestinal epithelial barrier function	1224:1261	intestinal epithelial barrier function	1224:1261	Our results show that targeting SNX10 may be a new therapeutic approach for restoring intestinal epithelial barrier function and promising strategy for IBD treatment.
34747049	5	37	theme	downstream	1116:1125	arg1	signaling					1127:1135	downstream signaling	1116:1135	downstream signaling	1116:1135	SNX10 deletion or treatment with DC-SX029, a novel SNX10 inhibitor, rescues OMV-induced intestinal barrier dysfunction and ameliorates colitis in mice by blocking cytosolic LPS release, caspase-5 activation, and downstream signaling.
34747049	1	38	theme	microbial	129:137	arg1	composition					139:149	Altered intestinal microbial composition	110:149	Altered intestinal microbial composition	110:149	Altered intestinal microbial composition promotes intestinal barrier dysfunction and triggers the initiation and recurrence of inflammatory bowel disease (IBD).
34747049	1	39	theme	bowel	250:254	arg1	IBD					265:267	IBD	265:267	IBD	265:267	Altered intestinal microbial composition promotes intestinal barrier dysfunction and triggers the initiation and recurrence of inflammatory bowel disease (IBD).
34747049	1	39	theme	bowel	250:254	arg1	disease					256:262	inflammatory bowel disease	237:262	inflammatory bowel disease (IBD)	237:268	Altered intestinal microbial composition promotes intestinal barrier dysfunction and triggers the initiation and recurrence of inflammatory bowel disease (IBD).
34747049	0	40	theme	barrier	45:51	arg1	dysfunction					53:63	intestinal barrier dysfunction	34:63	intestinal barrier dysfunction	34:63	SNX10-mediated LPS sensing causes intestinal barrier dysfunction via a caspase-5-dependent signaling cascade.
34747049	5	41	theme	SNX10	955:959	arg1	deletion					910:917	SNX10 deletion	904:917	SNX10 deletion	904:917	SNX10 deletion or treatment with DC-SX029, a novel SNX10 inhibitor, rescues OMV-induced intestinal barrier dysfunction and ameliorates colitis in mice by blocking cytosolic LPS release, caspase-5 activation, and downstream signaling.
34747049	5	41	theme	SNX10	955:959	arg1	inhibitor					961:969	a novel SNX10 inhibitor	947:969	a novel SNX10 inhibitor	947:969	SNX10 deletion or treatment with DC-SX029, a novel SNX10 inhibitor, rescues OMV-induced intestinal barrier dysfunction and ameliorates colitis in mice by blocking cytosolic LPS release, caspase-5 activation, and downstream signaling.
34747049	2	42	theme	Current	271:277	arg1	treatments					279:288	Current treatments	271:288	Current treatments for IBD	271:296	Current treatments for IBD are focused on control of inflammation rather than on maintaining intestinal epithelial barrier function.
34747049	1	43	theme	disease	256:262	arg1	recurrence					223:232	recurrence	223:232	recurrence	223:232	Altered intestinal microbial composition promotes intestinal barrier dysfunction and triggers the initiation and recurrence of inflammatory bowel disease (IBD).
34747049	1	43	theme	disease	256:262	arg1	initiation					208:217	initiation	208:217	initiation	208:217	Altered intestinal microbial composition promotes intestinal barrier dysfunction and triggers the initiation and recurrence of inflammatory bowel disease (IBD).
34747049	0	44	theme	intestinal	34:43	arg1	dysfunction					53:63	intestinal barrier dysfunction	34:63	intestinal barrier dysfunction	34:63	SNX10-mediated LPS sensing causes intestinal barrier dysfunction via a caspase-5-dependent signaling cascade.
34747049	3	45	theme	bacterial	460:468	arg1	vesicles					485:492	Gram-negative bacterial outer membrane vesicles	446:492	Gram-negative bacterial outer membrane vesicles (OMVs)	446:499	Here, we show that the internalization of Gram-negative bacterial outer membrane vesicles (OMVs) in human intestinal epithelial cells promotes recruitment of caspase-5 and PIKfyve to early endosomal membranes via sorting nexin 10 (SNX10), resulting in LPS release from OMVs into the cytosol.
34747049	3	45	theme	bacterial	460:468	arg1	OMVs					495:498	OMVs	495:498	OMVs	495:498	Here, we show that the internalization of Gram-negative bacterial outer membrane vesicles (OMVs) in human intestinal epithelial cells promotes recruitment of caspase-5 and PIKfyve to early endosomal membranes via sorting nexin 10 (SNX10), resulting in LPS release from OMVs into the cytosol.
34747049	4	46	theme	E-cadherin	845:854	arg1	expression					856:865	E-cadherin expression	845:865	E-cadherin expression	845:865	Caspase-5 activated by cytosolic LPS leads to Lyn phosphorylation, which in turn promotes nuclear translocalization of Snail/Slug, downregulation of E-cadherin expression, and intestinal barrier dysfunction.
34747049	1	47	dep	initiation	208:217	arg1	the					204:206	the	204:206	the	204:206	Altered intestinal microbial composition promotes intestinal barrier dysfunction and triggers the initiation and recurrence of inflammatory bowel disease (IBD).
34747049	6	48	theme	targeting	1160:1168	arg1	approach					1201:1208	a new therapeutic approach	1183:1208	a new therapeutic approach for restoring intestinal epithelial barrier function and promising strategy for IBD treatment	1183:1302	Our results show that targeting SNX10 may be a new therapeutic approach for restoring intestinal epithelial barrier function and promising strategy for IBD treatment.
34747049	6	48	theme	targeting	1160:1168	arg1	SNX10					1170:1174	targeting SNX10	1160:1174	targeting SNX10	1160:1174	Our results show that targeting SNX10 may be a new therapeutic approach for restoring intestinal epithelial barrier function and promising strategy for IBD treatment.
34747049	2	49	theme	barrier	386:392	arg1	function					394:401	intestinal epithelial barrier function	364:401	intestinal epithelial barrier function	364:401	Current treatments for IBD are focused on control of inflammation rather than on maintaining intestinal epithelial barrier function.
34747049	3	50	theme	intestinal	510:519	arg1	cells					532:536	human intestinal epithelial cells	504:536	human intestinal epithelial cells	504:536	Here, we show that the internalization of Gram-negative bacterial outer membrane vesicles (OMVs) in human intestinal epithelial cells promotes recruitment of caspase-5 and PIKfyve to early endosomal membranes via sorting nexin 10 (SNX10), resulting in LPS release from OMVs into the cytosol.
34747049	5	51	theme	caspase-5	1090:1098	arg1	activation					1100:1109	caspase-5 activation	1090:1109	caspase-5 activation	1090:1109	SNX10 deletion or treatment with DC-SX029, a novel SNX10 inhibitor, rescues OMV-induced intestinal barrier dysfunction and ameliorates colitis in mice by blocking cytosolic LPS release, caspase-5 activation, and downstream signaling.
34747049	4	52	theme	intestinal	872:881	arg1	dysfunction					891:901	intestinal barrier dysfunction	872:901	intestinal barrier dysfunction	872:901	Caspase-5 activated by cytosolic LPS leads to Lyn phosphorylation, which in turn promotes nuclear translocalization of Snail/Slug, downregulation of E-cadherin expression, and intestinal barrier dysfunction.
34747049	2	53	theme	epithelial	375:384	arg1	function					394:401	intestinal epithelial barrier function	364:401	intestinal epithelial barrier function	364:401	Current treatments for IBD are focused on control of inflammation rather than on maintaining intestinal epithelial barrier function.
34747049	5	54	theme	LPS	1077:1079	arg1	release					1081:1087	cytosolic LPS release	1067:1087	cytosolic LPS release	1067:1087	SNX10 deletion or treatment with DC-SX029, a novel SNX10 inhibitor, rescues OMV-induced intestinal barrier dysfunction and ameliorates colitis in mice by blocking cytosolic LPS release, caspase-5 activation, and downstream signaling.
34747049	1	55	theme	intestinal	160:169	arg1	dysfunction					179:189	intestinal barrier dysfunction	160:189	intestinal barrier dysfunction	160:189	Altered intestinal microbial composition promotes intestinal barrier dysfunction and triggers the initiation and recurrence of inflammatory bowel disease (IBD).
34747049	5	56	theme	intestinal	992:1001	arg1	dysfunction					1011:1021	OMV-induced intestinal barrier dysfunction	980:1021	OMV-induced intestinal barrier dysfunction	980:1021	SNX10 deletion or treatment with DC-SX029, a novel SNX10 inhibitor, rescues OMV-induced intestinal barrier dysfunction and ameliorates colitis in mice by blocking cytosolic LPS release, caspase-5 activation, and downstream signaling.
34747049	5	57	with	deletion	910:917	arg1	DC-SX029					937:944	DC-SX029	937:944	DC-SX029	937:944	SNX10 deletion or treatment with DC-SX029, a novel SNX10 inhibitor, rescues OMV-induced intestinal barrier dysfunction and ameliorates colitis in mice by blocking cytosolic LPS release, caspase-5 activation, and downstream signaling.
34747049	3	58	theme	LPS	656:658	arg1	release					660:666	LPS release	656:666	LPS release from OMVs into the cytosol	656:693	Here, we show that the internalization of Gram-negative bacterial outer membrane vesicles (OMVs) in human intestinal epithelial cells promotes recruitment of caspase-5 and PIKfyve to early endosomal membranes via sorting nexin 10 (SNX10), resulting in LPS release from OMVs into the cytosol.
34747049	1	59	theme	barrier	171:177	arg1	dysfunction					179:189	intestinal barrier dysfunction	160:189	intestinal barrier dysfunction	160:189	Altered intestinal microbial composition promotes intestinal barrier dysfunction and triggers the initiation and recurrence of inflammatory bowel disease (IBD).
34747049	2	60	theme	intestinal	364:373	arg1	function					394:401	intestinal epithelial barrier function	364:401	intestinal epithelial barrier function	364:401	Current treatments for IBD are focused on control of inflammation rather than on maintaining intestinal epithelial barrier function.
34747049	0	61	theme	caspase-5-dependent	71:89	arg1	cascade					101:107	a caspase-5-dependent signaling cascade	69:107	a caspase-5-dependent signaling cascade	69:107	SNX10-mediated LPS sensing causes intestinal barrier dysfunction via a caspase-5-dependent signaling cascade.
34747049	3	62	theme	caspase-5	562:570	arg1	recruitment					547:557	recruitment	547:557	recruitment of caspase-5 and PIKfyve to early endosomal membranes	547:611	Here, we show that the internalization of Gram-negative bacterial outer membrane vesicles (OMVs) in human intestinal epithelial cells promotes recruitment of caspase-5 and PIKfyve to early endosomal membranes via sorting nexin 10 (SNX10), resulting in LPS release from OMVs into the cytosol.
34747049	3	63	from	internalization	427:441	arg1	cells					532:536	human intestinal epithelial cells	504:536	human intestinal epithelial cells	504:536	Here, we show that the internalization of Gram-negative bacterial outer membrane vesicles (OMVs) in human intestinal epithelial cells promotes recruitment of caspase-5 and PIKfyve to early endosomal membranes via sorting nexin 10 (SNX10), resulting in LPS release from OMVs into the cytosol.
34384804	6	0	theme	sustained	1001:1009	arg1	release					1011:1017	sustained release	1001:1017	sustained release	1001:1017	Biphasic pattern, with initial rapid release followed by sustained release illustrated controlled delivery of linalool from chitosan nanocomposite, a prerequisite for shelf-life enhancement of stored food products.
34384804	5	1	from	temperatures	930:941	arg1	loss					914:917	reduced weight loss	899:917	reduced weight loss at varying temperatures	899:941	TGA analysis reflected the stability of Nm-linalool with reduced weight loss at varying temperatures.
34384804	9	2	theme	controlled	1625:1634	arg1	system					1650:1655	an innovative controlled nano-delivery system	1611:1655	an innovative controlled nano-delivery system for its practical application as novel environmentally friendly eco-smart preservative in food and agricultural industries	1611:1778	In conclusion, the investigation strengthens the application of chitosan-linalool nanocomposite as an innovative controlled nano-delivery system for its practical application as novel environmentally friendly eco-smart preservative in food and agricultural industries.
34384804	6	3	theme	rapid	975:979	arg1	release					981:987	initial rapid release	967:987	initial rapid release followed by sustained release	967:1017	Biphasic pattern, with initial rapid release followed by sustained release illustrated controlled delivery of linalool from chitosan nanocomposite, a prerequisite for shelf-life enhancement of stored food products.
34384804	8	4	theme	considerable	1395:1406	arg1	reduction					1408:1416	considerable reduction	1395:1416	considerable reduction of lipid peroxidation in rice	1395:1446	Further, Nm-linalool displayed considerable reduction of lipid peroxidation in rice without exerting any adverse impact on organoleptic attributes.
34384804	7	5	theme	antiaflatoxigenic	1240:1256	arg1	activity					1258:1265	prominent antifungal and antiaflatoxigenic activity	1215:1265	prominent antifungal and antiaflatoxigenic activity	1215:1265	Chitosan nanocomposite incorporating linalool displayed prominent antifungal and antiaflatoxigenic activity during in vitro as well as in situ investigation in rice with improved antioxidant potentiality.
34384804	9	6	from	preservative	1731:1742	arg1	industries					1769:1778	food and agricultural industries	1747:1778	food and agricultural industries	1747:1778	In conclusion, the investigation strengthens the application of chitosan-linalool nanocomposite as an innovative controlled nano-delivery system for its practical application as novel environmentally friendly eco-smart preservative in food and agricultural industries.
34384804	7	7	theme	antifungal	1225:1234	arg1	activity					1258:1265	prominent antifungal and antiaflatoxigenic activity	1215:1265	prominent antifungal and antiaflatoxigenic activity	1215:1265	Chitosan nanocomposite incorporating linalool displayed prominent antifungal and antiaflatoxigenic activity during in vitro as well as in situ investigation in rice with improved antioxidant potentiality.
34384804	1	8	theme	study	213:217	arg1	aim					194:196	The aim	190:196	The aim of the present study	190:217	The aim of the present study was to encapsulate linalool into chitosan nanocomposite (Nm-linalool) for developing novel controlled release delivery system in order to protect stored rice against fungal infestation, aflatoxin B1 (AFB1) contamination, and lipid peroxidation.
34384804	6	9	theme	chitosan	1068:1075	arg1	prerequisite					1094:1105	a prerequisite	1092:1105	a prerequisite for shelf-life enhancement of stored food products	1092:1156	Biphasic pattern, with initial rapid release followed by sustained release illustrated controlled delivery of linalool from chitosan nanocomposite, a prerequisite for shelf-life enhancement of stored food products.
34384804	6	9	theme	chitosan	1068:1075	arg1	nanocomposite					1077:1089	chitosan nanocomposite	1068:1089	chitosan nanocomposite	1068:1089	Biphasic pattern, with initial rapid release followed by sustained release illustrated controlled delivery of linalool from chitosan nanocomposite, a prerequisite for shelf-life enhancement of stored food products.
34384804	2	10	theme	AFM	591:593	arg1	investigation					595:607	AFM investigation	591:607	AFM investigation	591:607	The chitosan-linalool nanocomposite showed spherical shapes, smooth surface with monomodal distribution as revealed by SEM and AFM investigation.
34384804	8	11	theme	peroxidation	1427:1438	arg1	reduction					1408:1416	considerable reduction	1395:1416	considerable reduction of lipid peroxidation in rice	1395:1446	Further, Nm-linalool displayed considerable reduction of lipid peroxidation in rice without exerting any adverse impact on organoleptic attributes.
34384804	0	12	theme	delivery	151:158	arg1	system					160:165	innovative controlled release delivery system	121:165	innovative controlled release delivery system for food preservation	121:187	Fabrication, physico-chemical characterization, and bioactivity evaluation of chitosan-linalool composite nano-matrix as innovative controlled release delivery system for food preservation.
34384804	1	13	theme	B1	415:416	arg1	contamination					425:437	aflatoxin B1 (AFB1) contamination	405:437	aflatoxin B1 (AFB1) contamination	405:437	The aim of the present study was to encapsulate linalool into chitosan nanocomposite (Nm-linalool) for developing novel controlled release delivery system in order to protect stored rice against fungal infestation, aflatoxin B1 (AFB1) contamination, and lipid peroxidation.
34384804	6	14	theme	linalool	1054:1061	arg1	delivery					1042:1049	controlled delivery	1031:1049	controlled delivery of linalool from chitosan nanocomposite, a prerequisite for shelf-life enhancement of stored food products	1031:1156	Biphasic pattern, with initial rapid release followed by sustained release illustrated controlled delivery of linalool from chitosan nanocomposite, a prerequisite for shelf-life enhancement of stored food products.
34384804	9	15	theme	novel	1690:1694	arg1	preservative					1731:1742	novel environmentally friendly eco-smart preservative	1690:1742	novel environmentally friendly eco-smart preservative in food and agricultural industries	1690:1778	In conclusion, the investigation strengthens the application of chitosan-linalool nanocomposite as an innovative controlled nano-delivery system for its practical application as novel environmentally friendly eco-smart preservative in food and agricultural industries.
34384804	7	16	theme	Chitosan	1159:1166	arg1	nanocomposite					1168:1180	Chitosan nanocomposite	1159:1180	Chitosan nanocomposite incorporating linalool	1159:1203	Chitosan nanocomposite incorporating linalool displayed prominent antifungal and antiaflatoxigenic activity during in vitro as well as in situ investigation in rice with improved antioxidant potentiality.
34384804	9	17	theme	friendly	1712:1719	arg1	preservative					1731:1742	novel environmentally friendly eco-smart preservative	1690:1742	novel environmentally friendly eco-smart preservative in food and agricultural industries	1690:1778	In conclusion, the investigation strengthens the application of chitosan-linalool nanocomposite as an innovative controlled nano-delivery system for its practical application as novel environmentally friendly eco-smart preservative in food and agricultural industries.
34384804	4	18	theme	zeta	793:796	arg1	potential					798:806	higher zeta potential	786:806	higher zeta potential	786:806	Nanoencapsulation of linalool showed higher zeta potential and lowered polydispersity index.
34384804	8	19	theme	adverse	1469:1475	arg1	impact					1477:1482	any adverse impact	1465:1482	any adverse impact on organoleptic attributes	1465:1509	Further, Nm-linalool displayed considerable reduction of lipid peroxidation in rice without exerting any adverse impact on organoleptic attributes.
34384804	5	20	theme	weight	907:912	arg1	loss					914:917	reduced weight loss	899:917	reduced weight loss at varying temperatures	899:941	TGA analysis reflected the stability of Nm-linalool with reduced weight loss at varying temperatures.
34384804	2	21	with	shapes	517:522	arg1	distribution					555:566	monomodal distribution	545:566	monomodal distribution as revealed by SEM	545:585	The chitosan-linalool nanocomposite showed spherical shapes, smooth surface with monomodal distribution as revealed by SEM and AFM investigation.
34384804	9	22	theme	food	1747:1750	arg1	industries					1769:1778	food and agricultural industries	1747:1778	food and agricultural industries	1747:1778	In conclusion, the investigation strengthens the application of chitosan-linalool nanocomposite as an innovative controlled nano-delivery system for its practical application as novel environmentally friendly eco-smart preservative in food and agricultural industries.
34384804	6	23	theme	shelf-life	1111:1120	arg1	enhancement					1122:1132	shelf-life enhancement	1111:1132	shelf-life enhancement of stored food products	1111:1156	Biphasic pattern, with initial rapid release followed by sustained release illustrated controlled delivery of linalool from chitosan nanocomposite, a prerequisite for shelf-life enhancement of stored food products.
34384804	2	24	theme	smooth	525:530	arg1	surface					532:538	smooth surface	525:538	smooth surface with monomodal distribution as revealed by SEM	525:585	The chitosan-linalool nanocomposite showed spherical shapes, smooth surface with monomodal distribution as revealed by SEM and AFM investigation.
34384804	0	25	theme	composite	96:104	arg1	nano-matrix					106:116	chitosan-linalool composite nano-matrix	78:116	chitosan-linalool composite nano-matrix	78:116	Fabrication, physico-chemical characterization, and bioactivity evaluation of chitosan-linalool composite nano-matrix as innovative controlled release delivery system for food preservation.
34384804	9	26	theme	agricultural	1756:1767	arg1	industries					1769:1778	food and agricultural industries	1747:1778	food and agricultural industries	1747:1778	In conclusion, the investigation strengthens the application of chitosan-linalool nanocomposite as an innovative controlled nano-delivery system for its practical application as novel environmentally friendly eco-smart preservative in food and agricultural industries.
34384804	1	27	theme	fungal	385:390	arg1	infestation					392:402	fungal infestation	385:402	fungal infestation	385:402	The aim of the present study was to encapsulate linalool into chitosan nanocomposite (Nm-linalool) for developing novel controlled release delivery system in order to protect stored rice against fungal infestation, aflatoxin B1 (AFB1) contamination, and lipid peroxidation.
34384804	7	28	from	investigation	1302:1314	arg1	rice					1319:1322	rice	1319:1322	rice with improved antioxidant potentiality	1319:1361	Chitosan nanocomposite incorporating linalool displayed prominent antifungal and antiaflatoxigenic activity during in vitro as well as in situ investigation in rice with improved antioxidant potentiality.
34384804	3	29	from	changes	653:659	arg1	degree					664:669	degree	664:669	degree of crystallinity	664:686	FTIR and XRD represented peak shifting and changes in degree of crystallinity after incorporation of linalool into chitosan nanocomposite.
34384804	0	30	theme	controlled	132:141	arg1	system					160:165	innovative controlled release delivery system	121:165	innovative controlled release delivery system for food preservation	121:187	Fabrication, physico-chemical characterization, and bioactivity evaluation of chitosan-linalool composite nano-matrix as innovative controlled release delivery system for food preservation.
34384804	2	31	theme	chitosan-linalool	468:484	arg1	nanocomposite					486:498	The chitosan-linalool nanocomposite	464:498	The chitosan-linalool nanocomposite	464:498	The chitosan-linalool nanocomposite showed spherical shapes, smooth surface with monomodal distribution as revealed by SEM and AFM investigation.
34384804	1	32	theme	novel	304:308	arg1	system					338:343	novel controlled release delivery system	304:343	novel controlled release delivery system in order to protect stored rice against fungal infestation, aflatoxin B1 (AFB1) contamination, and lipid peroxidation	304:461	The aim of the present study was to encapsulate linalool into chitosan nanocomposite (Nm-linalool) for developing novel controlled release delivery system in order to protect stored rice against fungal infestation, aflatoxin B1 (AFB1) contamination, and lipid peroxidation.
34384804	6	33	with	pattern	953:959	arg1	release					981:987	initial rapid release	967:987	initial rapid release followed by sustained release	967:1017	Biphasic pattern, with initial rapid release followed by sustained release illustrated controlled delivery of linalool from chitosan nanocomposite, a prerequisite for shelf-life enhancement of stored food products.
34384804	4	34	theme	polydispersity	820:833	arg1	index					835:839	polydispersity index	820:839	polydispersity index	820:839	Nanoencapsulation of linalool showed higher zeta potential and lowered polydispersity index.
34384804	0	35	theme	physico-chemical	13:28	arg1	characterization					30:45	physico-chemical characterization	13:45	physico-chemical characterization	13:45	Fabrication, physico-chemical characterization, and bioactivity evaluation of chitosan-linalool composite nano-matrix as innovative controlled release delivery system for food preservation.
34384804	1	36	theme	release	321:327	arg1	system					338:343	novel controlled release delivery system	304:343	novel controlled release delivery system in order to protect stored rice against fungal infestation, aflatoxin B1 (AFB1) contamination, and lipid peroxidation	304:461	The aim of the present study was to encapsulate linalool into chitosan nanocomposite (Nm-linalool) for developing novel controlled release delivery system in order to protect stored rice against fungal infestation, aflatoxin B1 (AFB1) contamination, and lipid peroxidation.
34384804	6	37	from	nanocomposite	1077:1089	arg1	delivery					1042:1049	controlled delivery	1031:1049	controlled delivery of linalool from chitosan nanocomposite, a prerequisite for shelf-life enhancement of stored food products	1031:1156	Biphasic pattern, with initial rapid release followed by sustained release illustrated controlled delivery of linalool from chitosan nanocomposite, a prerequisite for shelf-life enhancement of stored food products.
34384804	3	38	from	shifting	640:647	arg1	degree					664:669	degree	664:669	degree of crystallinity	664:686	FTIR and XRD represented peak shifting and changes in degree of crystallinity after incorporation of linalool into chitosan nanocomposite.
34384804	7	39	theme	in	1294:1295	arg1	investigation					1302:1314	in vitro as well as in situ investigation	1274:1314	in vitro as well as in situ investigation in rice with improved antioxidant potentiality	1274:1361	Chitosan nanocomposite incorporating linalool displayed prominent antifungal and antiaflatoxigenic activity during in vitro as well as in situ investigation in rice with improved antioxidant potentiality.
34384804	6	40	theme	Biphasic	944:951	arg1	pattern					953:959	Biphasic pattern	944:959	Biphasic pattern	944:959	Biphasic pattern, with initial rapid release followed by sustained release illustrated controlled delivery of linalool from chitosan nanocomposite, a prerequisite for shelf-life enhancement of stored food products.
34384804	6	41	theme	food	1144:1147	arg1	products					1149:1156	stored food products	1137:1156	stored food products	1137:1156	Biphasic pattern, with initial rapid release followed by sustained release illustrated controlled delivery of linalool from chitosan nanocomposite, a prerequisite for shelf-life enhancement of stored food products.
34384804	3	42	theme	crystallinity	674:686	arg1	degree					664:669	degree	664:669	degree of crystallinity	664:686	FTIR and XRD represented peak shifting and changes in degree of crystallinity after incorporation of linalool into chitosan nanocomposite.
34384804	0	43	theme	bioactivity	52:62	arg1	evaluation					64:73	bioactivity evaluation	52:73	bioactivity evaluation	52:73	Fabrication, physico-chemical characterization, and bioactivity evaluation of chitosan-linalool composite nano-matrix as innovative controlled release delivery system for food preservation.
34384804	3	44	theme	chitosan	725:732	arg1	nanocomposite					734:746	chitosan nanocomposite	725:746	chitosan nanocomposite	725:746	FTIR and XRD represented peak shifting and changes in degree of crystallinity after incorporation of linalool into chitosan nanocomposite.
34384804	3	45	theme	linalool	711:718	arg1	incorporation					694:706	incorporation	694:706	incorporation of linalool into chitosan nanocomposite	694:746	FTIR and XRD represented peak shifting and changes in degree of crystallinity after incorporation of linalool into chitosan nanocomposite.
34384804	9	46	theme	chitosan-linalool	1576:1592	arg1	nanocomposite					1594:1606	chitosan-linalool nanocomposite	1576:1606	chitosan-linalool nanocomposite	1576:1606	In conclusion, the investigation strengthens the application of chitosan-linalool nanocomposite as an innovative controlled nano-delivery system for its practical application as novel environmentally friendly eco-smart preservative in food and agricultural industries.
34384804	5	47	theme	TGA	842:844	arg1	analysis					846:853	TGA analysis	842:853	TGA analysis	842:853	TGA analysis reflected the stability of Nm-linalool with reduced weight loss at varying temperatures.
34384804	7	48	theme	in	1274:1275	arg1	investigation					1302:1314	in vitro as well as in situ investigation	1274:1314	in vitro as well as in situ investigation in rice with improved antioxidant potentiality	1274:1361	Chitosan nanocomposite incorporating linalool displayed prominent antifungal and antiaflatoxigenic activity during in vitro as well as in situ investigation in rice with improved antioxidant potentiality.
34384804	2	49	with	investigation	595:607	arg1	distribution					555:566	monomodal distribution	545:566	monomodal distribution as revealed by SEM	545:585	The chitosan-linalool nanocomposite showed spherical shapes, smooth surface with monomodal distribution as revealed by SEM and AFM investigation.
34384804	9	50	theme	innovative	1614:1623	arg1	system					1650:1655	an innovative controlled nano-delivery system	1611:1655	an innovative controlled nano-delivery system for its practical application as novel environmentally friendly eco-smart preservative in food and agricultural industries	1611:1778	In conclusion, the investigation strengthens the application of chitosan-linalool nanocomposite as an innovative controlled nano-delivery system for its practical application as novel environmentally friendly eco-smart preservative in food and agricultural industries.
34384804	9	51	theme	nano-delivery	1636:1648	arg1	system					1650:1655	an innovative controlled nano-delivery system	1611:1655	an innovative controlled nano-delivery system for its practical application as novel environmentally friendly eco-smart preservative in food and agricultural industries	1611:1778	In conclusion, the investigation strengthens the application of chitosan-linalool nanocomposite as an innovative controlled nano-delivery system for its practical application as novel environmentally friendly eco-smart preservative in food and agricultural industries.
34384804	6	52	theme	initial	967:973	arg1	release					981:987	initial rapid release	967:987	initial rapid release followed by sustained release	967:1017	Biphasic pattern, with initial rapid release followed by sustained release illustrated controlled delivery of linalool from chitosan nanocomposite, a prerequisite for shelf-life enhancement of stored food products.
34384804	7	53	theme	prominent	1215:1223	arg1	activity					1258:1265	prominent antifungal and antiaflatoxigenic activity	1215:1265	prominent antifungal and antiaflatoxigenic activity	1215:1265	Chitosan nanocomposite incorporating linalool displayed prominent antifungal and antiaflatoxigenic activity during in vitro as well as in situ investigation in rice with improved antioxidant potentiality.
34384804	1	54	theme	present	205:211	arg1	study					213:217	the present study	201:217	the present study	201:217	The aim of the present study was to encapsulate linalool into chitosan nanocomposite (Nm-linalool) for developing novel controlled release delivery system in order to protect stored rice against fungal infestation, aflatoxin B1 (AFB1) contamination, and lipid peroxidation.
34384804	7	55	dep	in	1274:1275	arg1	vitro					1277:1281	vitro	1277:1281	vitro	1277:1281	Chitosan nanocomposite incorporating linalool displayed prominent antifungal and antiaflatoxigenic activity during in vitro as well as in situ investigation in rice with improved antioxidant potentiality.
34384804	9	56	theme	practical	1665:1673	arg1	application					1675:1685	its practical application	1661:1685	its practical application as novel environmentally friendly eco-smart preservative in food and agricultural industries	1661:1778	In conclusion, the investigation strengthens the application of chitosan-linalool nanocomposite as an innovative controlled nano-delivery system for its practical application as novel environmentally friendly eco-smart preservative in food and agricultural industries.
34384804	7	57	with	rice	1319:1322	arg1	potentiality					1350:1361	improved antioxidant potentiality	1329:1361	improved antioxidant potentiality	1329:1361	Chitosan nanocomposite incorporating linalool displayed prominent antifungal and antiaflatoxigenic activity during in vitro as well as in situ investigation in rice with improved antioxidant potentiality.
34384804	8	58	theme	lipid	1421:1425	arg1	peroxidation					1427:1438	lipid peroxidation	1421:1438	lipid peroxidation	1421:1438	Further, Nm-linalool displayed considerable reduction of lipid peroxidation in rice without exerting any adverse impact on organoleptic attributes.
34384804	0	59	theme	food	171:174	arg1	preservation					176:187	food preservation	171:187	food preservation	171:187	Fabrication, physico-chemical characterization, and bioactivity evaluation of chitosan-linalool composite nano-matrix as innovative controlled release delivery system for food preservation.
34384804	3	60	theme	peak	635:638	arg1	shifting					640:647	peak shifting	635:647	peak shifting	635:647	FTIR and XRD represented peak shifting and changes in degree of crystallinity after incorporation of linalool into chitosan nanocomposite.
34384804	5	61	theme	varying	922:928	arg1	temperatures					930:941	varying temperatures	922:941	varying temperatures	922:941	TGA analysis reflected the stability of Nm-linalool with reduced weight loss at varying temperatures.
34384804	7	62	theme	antioxidant	1338:1348	arg1	potentiality					1350:1361	improved antioxidant potentiality	1329:1361	improved antioxidant potentiality	1329:1361	Chitosan nanocomposite incorporating linalool displayed prominent antifungal and antiaflatoxigenic activity during in vitro as well as in situ investigation in rice with improved antioxidant potentiality.
34384804	8	63	from	reduction	1408:1416	arg1	rice					1443:1446	rice	1443:1446	rice	1443:1446	Further, Nm-linalool displayed considerable reduction of lipid peroxidation in rice without exerting any adverse impact on organoleptic attributes.
34384804	9	64	theme	environmentally	1696:1710	arg1	preservative					1731:1742	novel environmentally friendly eco-smart preservative	1690:1742	novel environmentally friendly eco-smart preservative in food and agricultural industries	1690:1778	In conclusion, the investigation strengthens the application of chitosan-linalool nanocomposite as an innovative controlled nano-delivery system for its practical application as novel environmentally friendly eco-smart preservative in food and agricultural industries.
34384804	6	65	theme	controlled	1031:1040	arg1	delivery					1042:1049	controlled delivery	1031:1049	controlled delivery of linalool from chitosan nanocomposite, a prerequisite for shelf-life enhancement of stored food products	1031:1156	Biphasic pattern, with initial rapid release followed by sustained release illustrated controlled delivery of linalool from chitosan nanocomposite, a prerequisite for shelf-life enhancement of stored food products.
34384804	4	66	theme	linalool	770:777	arg1	Nanoencapsulation					749:765	Nanoencapsulation	749:765	Nanoencapsulation of linalool	749:777	Nanoencapsulation of linalool showed higher zeta potential and lowered polydispersity index.
34384804	9	67	theme	eco-smart	1721:1729	arg1	preservative					1731:1742	novel environmentally friendly eco-smart preservative	1690:1742	novel environmentally friendly eco-smart preservative in food and agricultural industries	1690:1778	In conclusion, the investigation strengthens the application of chitosan-linalool nanocomposite as an innovative controlled nano-delivery system for its practical application as novel environmentally friendly eco-smart preservative in food and agricultural industries.
34384804	6	68	theme	stored	1137:1142	arg1	products					1149:1156	stored food products	1137:1156	stored food products	1137:1156	Biphasic pattern, with initial rapid release followed by sustained release illustrated controlled delivery of linalool from chitosan nanocomposite, a prerequisite for shelf-life enhancement of stored food products.
34384804	4	69	theme	higher	786:791	arg1	potential					798:806	higher zeta potential	786:806	higher zeta potential	786:806	Nanoencapsulation of linalool showed higher zeta potential and lowered polydispersity index.
34384804	0	70	theme	chitosan-linalool	78:94	arg1	nano-matrix					106:116	chitosan-linalool composite nano-matrix	78:116	chitosan-linalool composite nano-matrix	78:116	Fabrication, physico-chemical characterization, and bioactivity evaluation of chitosan-linalool composite nano-matrix as innovative controlled release delivery system for food preservation.
34384804	1	71	theme	lipid	444:448	arg1	peroxidation					450:461	lipid peroxidation	444:461	lipid peroxidation	444:461	The aim of the present study was to encapsulate linalool into chitosan nanocomposite (Nm-linalool) for developing novel controlled release delivery system in order to protect stored rice against fungal infestation, aflatoxin B1 (AFB1) contamination, and lipid peroxidation.
34384804	0	72	theme	nano-matrix	106:116	arg1	Fabrication					0:10	Fabrication	0:10	Fabrication	0:10	Fabrication, physico-chemical characterization, and bioactivity evaluation of chitosan-linalool composite nano-matrix as innovative controlled release delivery system for food preservation.
34384804	0	72	theme	nano-matrix	106:116	arg1	characterization					30:45	physico-chemical characterization	13:45	physico-chemical characterization	13:45	Fabrication, physico-chemical characterization, and bioactivity evaluation of chitosan-linalool composite nano-matrix as innovative controlled release delivery system for food preservation.
34384804	0	72	theme	nano-matrix	106:116	arg1	evaluation					64:73	bioactivity evaluation	52:73	bioactivity evaluation	52:73	Fabrication, physico-chemical characterization, and bioactivity evaluation of chitosan-linalool composite nano-matrix as innovative controlled release delivery system for food preservation.
34384804	1	73	theme	stored	365:370	arg1	rice					372:375	stored rice	365:375	stored rice	365:375	The aim of the present study was to encapsulate linalool into chitosan nanocomposite (Nm-linalool) for developing novel controlled release delivery system in order to protect stored rice against fungal infestation, aflatoxin B1 (AFB1) contamination, and lipid peroxidation.
34384804	8	74	theme	organoleptic	1487:1498	arg1	attributes					1500:1509	organoleptic attributes	1487:1509	organoleptic attributes	1487:1509	Further, Nm-linalool displayed considerable reduction of lipid peroxidation in rice without exerting any adverse impact on organoleptic attributes.
34384804	0	75	theme	innovative	121:130	arg1	system					160:165	innovative controlled release delivery system	121:165	innovative controlled release delivery system for food preservation	121:187	Fabrication, physico-chemical characterization, and bioactivity evaluation of chitosan-linalool composite nano-matrix as innovative controlled release delivery system for food preservation.
34384804	0	76	theme	release	143:149	arg1	system					160:165	innovative controlled release delivery system	121:165	innovative controlled release delivery system for food preservation	121:187	Fabrication, physico-chemical characterization, and bioactivity evaluation of chitosan-linalool composite nano-matrix as innovative controlled release delivery system for food preservation.
34384804	2	77	with	surface	532:538	arg1	distribution					555:566	monomodal distribution	545:566	monomodal distribution as revealed by SEM	545:585	The chitosan-linalool nanocomposite showed spherical shapes, smooth surface with monomodal distribution as revealed by SEM and AFM investigation.
34384804	2	78	theme	monomodal	545:553	arg1	distribution					555:566	monomodal distribution	545:566	monomodal distribution as revealed by SEM	545:585	The chitosan-linalool nanocomposite showed spherical shapes, smooth surface with monomodal distribution as revealed by SEM and AFM investigation.
34384804	5	79	theme	Nm-linalool	882:892	arg1	stability					869:877	the stability	865:877	the stability of Nm-linalool	865:892	TGA analysis reflected the stability of Nm-linalool with reduced weight loss at varying temperatures.
34384804	1	80	theme	aflatoxin	405:413	arg1	AFB1					419:422	AFB1	419:422	AFB1	419:422	The aim of the present study was to encapsulate linalool into chitosan nanocomposite (Nm-linalool) for developing novel controlled release delivery system in order to protect stored rice against fungal infestation, aflatoxin B1 (AFB1) contamination, and lipid peroxidation.
34384804	1	80	theme	aflatoxin	405:413	arg1	B1					415:416	aflatoxin B1	405:416	aflatoxin B1 (AFB1) contamination	405:437	The aim of the present study was to encapsulate linalool into chitosan nanocomposite (Nm-linalool) for developing novel controlled release delivery system in order to protect stored rice against fungal infestation, aflatoxin B1 (AFB1) contamination, and lipid peroxidation.
34384804	7	81	dep	in	1294:1295	arg1	situ					1297:1300	situ	1297:1300	situ	1297:1300	Chitosan nanocomposite incorporating linalool displayed prominent antifungal and antiaflatoxigenic activity during in vitro as well as in situ investigation in rice with improved antioxidant potentiality.
34384804	5	82	theme	reduced	899:905	arg1	loss					914:917	reduced weight loss	899:917	reduced weight loss at varying temperatures	899:941	TGA analysis reflected the stability of Nm-linalool with reduced weight loss at varying temperatures.
34384804	1	83	theme	controlled	310:319	arg1	system					338:343	novel controlled release delivery system	304:343	novel controlled release delivery system in order to protect stored rice against fungal infestation, aflatoxin B1 (AFB1) contamination, and lipid peroxidation	304:461	The aim of the present study was to encapsulate linalool into chitosan nanocomposite (Nm-linalool) for developing novel controlled release delivery system in order to protect stored rice against fungal infestation, aflatoxin B1 (AFB1) contamination, and lipid peroxidation.
34384804	2	84	dep	distribution	555:566	arg1	revealed					571:578	revealed	571:578	revealed by SEM	571:585	The chitosan-linalool nanocomposite showed spherical shapes, smooth surface with monomodal distribution as revealed by SEM and AFM investigation.
34384804	8	85	from	impact	1477:1482	arg1	attributes					1500:1509	organoleptic attributes	1487:1509	organoleptic attributes	1487:1509	Further, Nm-linalool displayed considerable reduction of lipid peroxidation in rice without exerting any adverse impact on organoleptic attributes.
34384804	1	86	theme	delivery	329:336	arg1	system					338:343	novel controlled release delivery system	304:343	novel controlled release delivery system in order to protect stored rice against fungal infestation, aflatoxin B1 (AFB1) contamination, and lipid peroxidation	304:461	The aim of the present study was to encapsulate linalool into chitosan nanocomposite (Nm-linalool) for developing novel controlled release delivery system in order to protect stored rice against fungal infestation, aflatoxin B1 (AFB1) contamination, and lipid peroxidation.
34384804	6	87	theme	products	1149:1156	arg1	enhancement					1122:1132	shelf-life enhancement	1111:1132	shelf-life enhancement of stored food products	1111:1156	Biphasic pattern, with initial rapid release followed by sustained release illustrated controlled delivery of linalool from chitosan nanocomposite, a prerequisite for shelf-life enhancement of stored food products.
34384804	2	88	theme	spherical	507:515	arg1	shapes					517:522	spherical shapes	507:522	spherical shapes	507:522	The chitosan-linalool nanocomposite showed spherical shapes, smooth surface with monomodal distribution as revealed by SEM and AFM investigation.
34384804	7	89	theme	improved	1329:1336	arg1	potentiality					1350:1361	improved antioxidant potentiality	1329:1361	improved antioxidant potentiality	1329:1361	Chitosan nanocomposite incorporating linalool displayed prominent antifungal and antiaflatoxigenic activity during in vitro as well as in situ investigation in rice with improved antioxidant potentiality.
34384804	1	90	theme	chitosan	252:259	arg1	nanocomposite					261:273	chitosan nanocomposite	252:273	chitosan nanocomposite (Nm-linalool) for developing novel controlled release delivery system in order to protect stored rice against fungal infestation, aflatoxin B1 (AFB1) contamination, and lipid peroxidation	252:461	The aim of the present study was to encapsulate linalool into chitosan nanocomposite (Nm-linalool) for developing novel controlled release delivery system in order to protect stored rice against fungal infestation, aflatoxin B1 (AFB1) contamination, and lipid peroxidation.
34384804	1	90	theme	chitosan	252:259	arg1	Nm-linalool					276:286	Nm-linalool	276:286	Nm-linalool	276:286	The aim of the present study was to encapsulate linalool into chitosan nanocomposite (Nm-linalool) for developing novel controlled release delivery system in order to protect stored rice against fungal infestation, aflatoxin B1 (AFB1) contamination, and lipid peroxidation.
34384804	9	91	theme	nanocomposite	1594:1606	arg1	application					1561:1571	the application	1557:1571	the application of chitosan-linalool nanocomposite as an innovative controlled nano-delivery system for its practical application as novel environmentally friendly eco-smart preservative in food and agricultural industries	1557:1778	In conclusion, the investigation strengthens the application of chitosan-linalool nanocomposite as an innovative controlled nano-delivery system for its practical application as novel environmentally friendly eco-smart preservative in food and agricultural industries.
34069916	9	0	from	shift	1317:1321	arg1	abundance					1369:1377	the taxonomic and functional gene category abundance	1326:1377	the taxonomic and functional gene category abundance caused by dysbiosis, which occurs during the progression of periodontal disease	1326:1457	These results provide important insights into the shift in the taxonomic and functional gene category abundance caused by dysbiosis, which occurs during the progression of periodontal disease.
34069916	7	1	with	sites	968:972	arg1	pockets					991:997	periodontal pockets	979:997	periodontal pockets	979:997	In the taxonomic composition analysis, genus Bacteroides and Mycobacterium demonstrated significantly different compositions between healthy sites and sites with periodontal pockets.
34069916	4	2	theme	subgingival	574:584	arg1	microbiome					586:595	the subgingival microbiome	570:595	the subgingival microbiome	570:595	Previous studies have implicated dysbiosis of the subgingival microbiome in the etiology of periodontitis.
34069916	8	3	theme	significant	1185:1195	arg1	differences					1197:1207	significant differences	1185:1207	significant differences between healthy sites and sites with periodontal pockets	1185:1264	The results from the relative abundance of functional gene categories, carbohydrate metabolism, glycan biosynthesis and metabolism, amino acid metabolism, replication and repair showed significant differences between healthy sites and sites with periodontal pockets.
34069916	9	4	theme	gene	1355:1358	arg1	abundance					1369:1377	the taxonomic and functional gene category abundance	1326:1377	the taxonomic and functional gene category abundance caused by dysbiosis, which occurs during the progression of periodontal disease	1326:1457	These results provide important insights into the shift in the taxonomic and functional gene category abundance caused by dysbiosis, which occurs during the progression of periodontal disease.
34069916	8	5	theme	categories	1059:1068	arg1	abundance					1030:1038	the relative abundance	1017:1038	the relative abundance of functional gene categories, carbohydrate metabolism, glycan biosynthesis and metabolism, amino acid metabolism, replication and repair	1017:1176	The results from the relative abundance of functional gene categories, carbohydrate metabolism, glycan biosynthesis and metabolism, amino acid metabolism, replication and repair showed significant differences between healthy sites and sites with periodontal pockets.
34069916	3	6	from	microbiome	486:495	arg1	plaque					516:521	the subgingival plaque	500:521	the subgingival plaque	500:521	The development of next-generation sequencing made it possible to investigate the whole microbiome in the subgingival plaque.
34069916	8	7	from	abundance	1030:1038	arg1	results					1004:1010	The results	1000:1010	The results from the relative abundance of functional gene categories, carbohydrate metabolism, glycan biosynthesis and metabolism, amino acid metabolism, replication and repair	1000:1176	The results from the relative abundance of functional gene categories, carbohydrate metabolism, glycan biosynthesis and metabolism, amino acid metabolism, replication and repair showed significant differences between healthy sites and sites with periodontal pockets.
34069916	0	8	theme	Individuals	85:95	arg1	Group					67:71	a Group	65:71	a Group of Japanese Individuals with and without Periodontitis	65:126	Taxonomic and Gene Category Analyses of Subgingival Plaques from a Group of Japanese Individuals with and without Periodontitis.
34069916	0	8	theme	Individuals	85:95	arg1	Individuals					85:95	Japanese Individuals	76:95	Japanese Individuals with and without Periodontitis	76:126	Taxonomic and Gene Category Analyses of Subgingival Plaques from a Group of Japanese Individuals with and without Periodontitis.
34069916	8	9	with	sites	1225:1229	arg1	pockets					1258:1264	periodontal pockets	1246:1264	periodontal pockets	1246:1264	The results from the relative abundance of functional gene categories, carbohydrate metabolism, glycan biosynthesis and metabolism, amino acid metabolism, replication and repair showed significant differences between healthy sites and sites with periodontal pockets.
34069916	8	10	theme	relative	1021:1028	arg1	abundance					1030:1038	the relative abundance	1017:1038	the relative abundance of functional gene categories, carbohydrate metabolism, glycan biosynthesis and metabolism, amino acid metabolism, replication and repair	1017:1176	The results from the relative abundance of functional gene categories, carbohydrate metabolism, glycan biosynthesis and metabolism, amino acid metabolism, replication and repair showed significant differences between healthy sites and sites with periodontal pockets.
34069916	9	11	theme	category	1360:1367	arg1	abundance					1369:1377	the taxonomic and functional gene category abundance	1326:1377	the taxonomic and functional gene category abundance caused by dysbiosis, which occurs during the progression of periodontal disease	1326:1457	These results provide important insights into the shift in the taxonomic and functional gene category abundance caused by dysbiosis, which occurs during the progression of periodontal disease.
34069916	0	12	theme	Japanese	76:83	arg1	Individuals					85:95	Japanese Individuals	76:95	Japanese Individuals with and without Periodontitis	76:126	Taxonomic and Gene Category Analyses of Subgingival Plaques from a Group of Japanese Individuals with and without Periodontitis.
34069916	8	13	theme	carbohydrate	1071:1082	arg1	categories					1059:1068	functional gene categories	1043:1068	functional gene categories	1043:1068	The results from the relative abundance of functional gene categories, carbohydrate metabolism, glycan biosynthesis and metabolism, amino acid metabolism, replication and repair showed significant differences between healthy sites and sites with periodontal pockets.
34069916	8	13	theme	carbohydrate	1071:1082	arg1	metabolism					1084:1093	carbohydrate metabolism	1071:1093	carbohydrate metabolism	1071:1093	The results from the relative abundance of functional gene categories, carbohydrate metabolism, glycan biosynthesis and metabolism, amino acid metabolism, replication and repair showed significant differences between healthy sites and sites with periodontal pockets.
34069916	7	14	theme	periodontal	979:989	arg1	pockets					991:997	periodontal pockets	979:997	periodontal pockets	979:997	In the taxonomic composition analysis, genus Bacteroides and Mycobacterium demonstrated significantly different compositions between healthy sites and sites with periodontal pockets.
34069916	6	15	theme	metagenomic	697:707	arg1	analysis					709:716	a metagenomic analysis	695:716	a metagenomic analysis of subgingival plaque samples from a group of Japanese individuals with and without periodontitis	695:814	In this study, we conducted a metagenomic analysis of subgingival plaque samples from a group of Japanese individuals with and without periodontitis.
34069916	4	16	theme	microbiome	586:595	arg1	dysbiosis					557:565	dysbiosis	557:565	dysbiosis of the subgingival microbiome	557:595	Previous studies have implicated dysbiosis of the subgingival microbiome in the etiology of periodontitis.
34069916	7	17	theme	genus	856:860	arg1	Bacteroides					862:872	genus Bacteroides and Mycobacterium	856:890	Bacteroides	862:872	In the taxonomic composition analysis, genus Bacteroides and Mycobacterium demonstrated significantly different compositions between healthy sites and sites with periodontal pockets.
34069916	8	18	theme	glycan	1096:1101	arg1	categories					1059:1068	functional gene categories	1043:1068	functional gene categories	1043:1068	The results from the relative abundance of functional gene categories, carbohydrate metabolism, glycan biosynthesis and metabolism, amino acid metabolism, replication and repair showed significant differences between healthy sites and sites with periodontal pockets.
34069916	8	18	theme	glycan	1096:1101	arg1	biosynthesis					1103:1114	glycan biosynthesis	1096:1114	glycan biosynthesis	1096:1114	The results from the relative abundance of functional gene categories, carbohydrate metabolism, glycan biosynthesis and metabolism, amino acid metabolism, replication and repair showed significant differences between healthy sites and sites with periodontal pockets.
34069916	1	19	theme	tooth-supporting	165:180	arg1	tissues					182:188	tooth-supporting tissues	165:188	tooth-supporting tissues	165:188	Periodontitis is an inflammation of tooth-supporting tissues, which is caused by bacteria in the subgingival plaque (biofilm) and the host immune response.
34069916	1	20	theme	tissues	182:188	arg1	Periodontitis					129:141	Periodontitis	129:141	Periodontitis	129:141	Periodontitis is an inflammation of tooth-supporting tissues, which is caused by bacteria in the subgingival plaque (biofilm) and the host immune response.
34069916	1	20	theme	tissues	182:188	arg1	inflammation					149:160	an inflammation	146:160	an inflammation	146:160	Periodontitis is an inflammation of tooth-supporting tissues, which is caused by bacteria in the subgingival plaque (biofilm) and the host immune response.
34069916	2	21	theme	DNA	378:380	arg1	probes					382:387	DNA probes	378:387	DNA probes	378:387	Traditionally, subgingival pathogens have been investigated using methods such as culturing, DNA probes, or PCR.
34069916	6	22	theme	individuals	773:783	arg1	individuals					773:783	Japanese individuals	764:783	Japanese individuals with and without periodontitis	764:814	In this study, we conducted a metagenomic analysis of subgingival plaque samples from a group of Japanese individuals with and without periodontitis.
34069916	6	22	theme	individuals	773:783	arg1	group					755:759	a group	753:759	a group of Japanese individuals with and without periodontitis	753:814	In this study, we conducted a metagenomic analysis of subgingival plaque samples from a group of Japanese individuals with and without periodontitis.
34069916	2	23	theme	subgingival	300:310	arg1	pathogens					312:320	subgingival pathogens	300:320	subgingival pathogens	300:320	Traditionally, subgingival pathogens have been investigated using methods such as culturing, DNA probes, or PCR.
34069916	0	24	theme	Taxonomic	0:8	arg1	Analyses					28:35	Taxonomic and Gene Category Analyses	0:35	Analyses	28:35	Taxonomic and Gene Category Analyses of Subgingival Plaques from a Group of Japanese Individuals with and without Periodontitis.
34069916	7	25	with	sites	958:962	arg1	pockets					991:997	periodontal pockets	979:997	periodontal pockets	979:997	In the taxonomic composition analysis, genus Bacteroides and Mycobacterium demonstrated significantly different compositions between healthy sites and sites with periodontal pockets.
34069916	6	26	theme	Japanese	764:771	arg1	individuals					773:783	Japanese individuals	764:783	Japanese individuals with and without periodontitis	764:814	In this study, we conducted a metagenomic analysis of subgingival plaque samples from a group of Japanese individuals with and without periodontitis.
34069916	7	27	theme	taxonomic	824:832	arg1	analysis					846:853	the taxonomic composition analysis	820:853	the taxonomic composition analysis	820:853	In the taxonomic composition analysis, genus Bacteroides and Mycobacterium demonstrated significantly different compositions between healthy sites and sites with periodontal pockets.
34069916	8	28	theme	gene	1054:1057	arg1	categories					1059:1068	functional gene categories	1043:1068	functional gene categories	1043:1068	The results from the relative abundance of functional gene categories, carbohydrate metabolism, glycan biosynthesis and metabolism, amino acid metabolism, replication and repair showed significant differences between healthy sites and sites with periodontal pockets.
34069916	8	28	theme	gene	1054:1057	arg1	metabolism					1084:1093	carbohydrate metabolism	1071:1093	carbohydrate metabolism	1071:1093	The results from the relative abundance of functional gene categories, carbohydrate metabolism, glycan biosynthesis and metabolism, amino acid metabolism, replication and repair showed significant differences between healthy sites and sites with periodontal pockets.
34069916	8	28	theme	gene	1054:1057	arg1	biosynthesis					1103:1114	glycan biosynthesis	1096:1114	glycan biosynthesis	1096:1114	The results from the relative abundance of functional gene categories, carbohydrate metabolism, glycan biosynthesis and metabolism, amino acid metabolism, replication and repair showed significant differences between healthy sites and sites with periodontal pockets.
34069916	8	28	theme	gene	1054:1057	arg1	metabolism					1120:1129	metabolism	1120:1129	metabolism	1120:1129	The results from the relative abundance of functional gene categories, carbohydrate metabolism, glycan biosynthesis and metabolism, amino acid metabolism, replication and repair showed significant differences between healthy sites and sites with periodontal pockets.
34069916	0	29	theme	Category	19:26	arg1	Analyses					28:35	Taxonomic and Gene Category Analyses	0:35	Analyses	28:35	Taxonomic and Gene Category Analyses of Subgingival Plaques from a Group of Japanese Individuals with and without Periodontitis.
34069916	1	30	theme	host	263:266	arg1	response					275:282	the host immune response	259:282	the host immune response	259:282	Periodontitis is an inflammation of tooth-supporting tissues, which is caused by bacteria in the subgingival plaque (biofilm) and the host immune response.
34069916	8	31	theme	functional	1043:1052	arg1	categories					1059:1068	functional gene categories	1043:1068	functional gene categories	1043:1068	The results from the relative abundance of functional gene categories, carbohydrate metabolism, glycan biosynthesis and metabolism, amino acid metabolism, replication and repair showed significant differences between healthy sites and sites with periodontal pockets.
34069916	8	31	theme	functional	1043:1052	arg1	metabolism					1084:1093	carbohydrate metabolism	1071:1093	carbohydrate metabolism	1071:1093	The results from the relative abundance of functional gene categories, carbohydrate metabolism, glycan biosynthesis and metabolism, amino acid metabolism, replication and repair showed significant differences between healthy sites and sites with periodontal pockets.
34069916	8	31	theme	functional	1043:1052	arg1	biosynthesis					1103:1114	glycan biosynthesis	1096:1114	glycan biosynthesis	1096:1114	The results from the relative abundance of functional gene categories, carbohydrate metabolism, glycan biosynthesis and metabolism, amino acid metabolism, replication and repair showed significant differences between healthy sites and sites with periodontal pockets.
34069916	8	31	theme	functional	1043:1052	arg1	metabolism					1120:1129	metabolism	1120:1129	metabolism	1120:1129	The results from the relative abundance of functional gene categories, carbohydrate metabolism, glycan biosynthesis and metabolism, amino acid metabolism, replication and repair showed significant differences between healthy sites and sites with periodontal pockets.
34069916	7	32	theme	composition	834:844	arg1	analysis					846:853	the taxonomic composition analysis	820:853	the taxonomic composition analysis	820:853	In the taxonomic composition analysis, genus Bacteroides and Mycobacterium demonstrated significantly different compositions between healthy sites and sites with periodontal pockets.
34069916	0	33	theme	Gene	14:17	arg1	Analyses					28:35	Taxonomic and Gene Category Analyses	0:35	Analyses	28:35	Taxonomic and Gene Category Analyses of Subgingival Plaques from a Group of Japanese Individuals with and without Periodontitis.
34069916	1	34	theme	immune	268:273	arg1	response					275:282	the host immune response	259:282	the host immune response	259:282	Periodontitis is an inflammation of tooth-supporting tissues, which is caused by bacteria in the subgingival plaque (biofilm) and the host immune response.
34069916	9	35	theme	important	1289:1297	arg1	insights					1299:1306	important insights	1289:1306	important insights into the shift in the taxonomic and functional gene category abundance caused by dysbiosis, which occurs during the progression of periodontal disease	1289:1457	These results provide important insights into the shift in the taxonomic and functional gene category abundance caused by dysbiosis, which occurs during the progression of periodontal disease.
34069916	8	36	theme	acid	1138:1141	arg1	metabolism					1120:1129	metabolism	1120:1129	metabolism	1120:1129	The results from the relative abundance of functional gene categories, carbohydrate metabolism, glycan biosynthesis and metabolism, amino acid metabolism, replication and repair showed significant differences between healthy sites and sites with periodontal pockets.
34069916	8	36	theme	acid	1138:1141	arg1	metabolism					1143:1152	amino acid metabolism	1132:1152	amino acid metabolism	1132:1152	The results from the relative abundance of functional gene categories, carbohydrate metabolism, glycan biosynthesis and metabolism, amino acid metabolism, replication and repair showed significant differences between healthy sites and sites with periodontal pockets.
34069916	0	37	from	Group	67:71	arg1	Analyses					28:35	Taxonomic and Gene Category Analyses	0:35	Analyses	28:35	Taxonomic and Gene Category Analyses of Subgingival Plaques from a Group of Japanese Individuals with and without Periodontitis.
34069916	3	38	theme	sequencing	433:442	arg1	development					402:412	The development	398:412	The development of next-generation sequencing	398:442	The development of next-generation sequencing made it possible to investigate the whole microbiome in the subgingival plaque.
34069916	8	39	theme	amino	1132:1136	arg1	metabolism					1120:1129	metabolism	1120:1129	metabolism	1120:1129	The results from the relative abundance of functional gene categories, carbohydrate metabolism, glycan biosynthesis and metabolism, amino acid metabolism, replication and repair showed significant differences between healthy sites and sites with periodontal pockets.
34069916	8	39	theme	amino	1132:1136	arg1	metabolism					1143:1152	amino acid metabolism	1132:1152	amino acid metabolism	1132:1152	The results from the relative abundance of functional gene categories, carbohydrate metabolism, glycan biosynthesis and metabolism, amino acid metabolism, replication and repair showed significant differences between healthy sites and sites with periodontal pockets.
34069916	4	40	theme	periodontitis	616:628	arg1	etiology					604:611	the etiology	600:611	the etiology of periodontitis	600:628	Previous studies have implicated dysbiosis of the subgingival microbiome in the etiology of periodontitis.
34069916	9	41	theme	periodontal	1439:1449	arg1	disease					1451:1457	periodontal disease	1439:1457	periodontal disease	1439:1457	These results provide important insights into the shift in the taxonomic and functional gene category abundance caused by dysbiosis, which occurs during the progression of periodontal disease.
34069916	0	42	theme	Plaques	52:58	arg1	Analyses					28:35	Taxonomic and Gene Category Analyses	0:35	Analyses	28:35	Taxonomic and Gene Category Analyses of Subgingival Plaques from a Group of Japanese Individuals with and without Periodontitis.
34069916	8	43	theme	healthy	1217:1223	arg1	sites					1225:1229	healthy sites	1217:1229	healthy sites	1217:1229	The results from the relative abundance of functional gene categories, carbohydrate metabolism, glycan biosynthesis and metabolism, amino acid metabolism, replication and repair showed significant differences between healthy sites and sites with periodontal pockets.
34069916	1	44	from	bacteria	210:217	arg1	biofilm					246:252	the subgingival plaque (biofilm)	222:253	the subgingival plaque (biofilm)	222:253	Periodontitis is an inflammation of tooth-supporting tissues, which is caused by bacteria in the subgingival plaque (biofilm) and the host immune response.
34069916	1	44	from	bacteria	210:217	arg1	response					275:282	the host immune response	259:282	the host immune response	259:282	Periodontitis is an inflammation of tooth-supporting tissues, which is caused by bacteria in the subgingival plaque (biofilm) and the host immune response.
34069916	0	45	theme	Subgingival	40:50	arg1	Plaques					52:58	Subgingival Plaques	40:58	Subgingival Plaques	40:58	Taxonomic and Gene Category Analyses of Subgingival Plaques from a Group of Japanese Individuals with and without Periodontitis.
34069916	9	46	theme	functional	1344:1353	arg1	abundance					1369:1377	the taxonomic and functional gene category abundance	1326:1377	the taxonomic and functional gene category abundance caused by dysbiosis, which occurs during the progression of periodontal disease	1326:1457	These results provide important insights into the shift in the taxonomic and functional gene category abundance caused by dysbiosis, which occurs during the progression of periodontal disease.
34069916	3	47	theme	whole	480:484	arg1	microbiome					486:495	the whole microbiome	476:495	the whole microbiome in the subgingival plaque	476:521	The development of next-generation sequencing made it possible to investigate the whole microbiome in the subgingival plaque.
34069916	4	48	theme	Previous	524:531	arg1	studies					533:539	Previous studies	524:539	Previous studies	524:539	Previous studies have implicated dysbiosis of the subgingival microbiome in the etiology of periodontitis.
34069916	6	49	theme	samples	740:746	arg1	analysis					709:716	a metagenomic analysis	695:716	a metagenomic analysis of subgingival plaque samples from a group of Japanese individuals with and without periodontitis	695:814	In this study, we conducted a metagenomic analysis of subgingival plaque samples from a group of Japanese individuals with and without periodontitis.
34069916	6	50	from	group	755:759	arg1	analysis					709:716	a metagenomic analysis	695:716	a metagenomic analysis of subgingival plaque samples from a group of Japanese individuals with and without periodontitis	695:814	In this study, we conducted a metagenomic analysis of subgingival plaque samples from a group of Japanese individuals with and without periodontitis.
34069916	6	50	from	group	755:759	arg1	samples					740:746	subgingival plaque samples	721:746	subgingival plaque samples from a group of Japanese individuals with and without periodontitis	721:814	In this study, we conducted a metagenomic analysis of subgingival plaque samples from a group of Japanese individuals with and without periodontitis.
34069916	7	51	theme	healthy	950:956	arg1	sites					958:962	healthy sites	950:962	healthy sites	950:962	In the taxonomic composition analysis, genus Bacteroides and Mycobacterium demonstrated significantly different compositions between healthy sites and sites with periodontal pockets.
34069916	9	52	theme	disease	1451:1457	arg1	progression					1424:1434	the progression	1420:1434	the progression of periodontal disease	1420:1457	These results provide important insights into the shift in the taxonomic and functional gene category abundance caused by dysbiosis, which occurs during the progression of periodontal disease.
34069916	8	53	with	sites	1235:1239	arg1	pockets					1258:1264	periodontal pockets	1246:1264	periodontal pockets	1246:1264	The results from the relative abundance of functional gene categories, carbohydrate metabolism, glycan biosynthesis and metabolism, amino acid metabolism, replication and repair showed significant differences between healthy sites and sites with periodontal pockets.
34069916	6	54	theme	plaque	733:738	arg1	samples					740:746	subgingival plaque samples	721:746	subgingival plaque samples from a group of Japanese individuals with and without periodontitis	721:814	In this study, we conducted a metagenomic analysis of subgingival plaque samples from a group of Japanese individuals with and without periodontitis.
34069916	6	55	from	analysis	709:716	arg1	individuals					773:783	Japanese individuals	764:783	Japanese individuals with and without periodontitis	764:814	In this study, we conducted a metagenomic analysis of subgingival plaque samples from a group of Japanese individuals with and without periodontitis.
34069916	6	55	from	analysis	709:716	arg1	group					755:759	a group	753:759	a group of Japanese individuals with and without periodontitis	753:814	In this study, we conducted a metagenomic analysis of subgingival plaque samples from a group of Japanese individuals with and without periodontitis.
34069916	7	56	theme	different	919:927	arg1	compositions					929:940	significantly different compositions	905:940	significantly different compositions between healthy sites and sites with periodontal pockets	905:997	In the taxonomic composition analysis, genus Bacteroides and Mycobacterium demonstrated significantly different compositions between healthy sites and sites with periodontal pockets.
34069916	9	57	theme	taxonomic	1330:1338	arg1	abundance					1369:1377	the taxonomic and functional gene category abundance	1326:1377	the taxonomic and functional gene category abundance caused by dysbiosis, which occurs during the progression of periodontal disease	1326:1457	These results provide important insights into the shift in the taxonomic and functional gene category abundance caused by dysbiosis, which occurs during the progression of periodontal disease.
34069916	6	58	theme	subgingival	721:731	arg1	samples					740:746	subgingival plaque samples	721:746	subgingival plaque samples from a group of Japanese individuals with and without periodontitis	721:814	In this study, we conducted a metagenomic analysis of subgingival plaque samples from a group of Japanese individuals with and without periodontitis.
34069916	3	59	theme	next-generation	417:431	arg1	sequencing					433:442	next-generation sequencing	417:442	next-generation sequencing	417:442	The development of next-generation sequencing made it possible to investigate the whole microbiome in the subgingival plaque.
34069916	1	60	theme	subgingival	226:236	arg1	biofilm					246:252	the subgingival plaque (biofilm)	222:253	the subgingival plaque (biofilm)	222:253	Periodontitis is an inflammation of tooth-supporting tissues, which is caused by bacteria in the subgingival plaque (biofilm) and the host immune response.
34069916	8	61	theme	periodontal	1246:1256	arg1	pockets					1258:1264	periodontal pockets	1246:1264	periodontal pockets	1246:1264	The results from the relative abundance of functional gene categories, carbohydrate metabolism, glycan biosynthesis and metabolism, amino acid metabolism, replication and repair showed significant differences between healthy sites and sites with periodontal pockets.
34069916	3	62	theme	subgingival	504:514	arg1	plaque					516:521	the subgingival plaque	500:521	the subgingival plaque	500:521	The development of next-generation sequencing made it possible to investigate the whole microbiome in the subgingival plaque.
34069916	1	63	theme	plaque	238:243	arg1	biofilm					246:252	the subgingival plaque (biofilm)	222:253	the subgingival plaque (biofilm)	222:253	Periodontitis is an inflammation of tooth-supporting tissues, which is caused by bacteria in the subgingival plaque (biofilm) and the host immune response.
33278972	1	0	contain	have	183:186	arg1	polymers					146:153	Biocompatible polymers	132:153	Biocompatible polymers	132:153	Biocompatible polymers and drug-delivery scaffolds have driven development in bone regeneration.
33278972	1	0	contain	have	183:186	arg2	development					195:205	driven development	188:205	driven development	188:205	Biocompatible polymers and drug-delivery scaffolds have driven development in bone regeneration.
33278972	1	0	contain	have	183:186	arg1	scaffolds					173:181	drug-delivery scaffolds	159:181	drug-delivery scaffolds	159:181	Biocompatible polymers and drug-delivery scaffolds have driven development in bone regeneration.
33278972	1	1	theme	bone	210:213	arg1	regeneration					215:226	bone regeneration	210:226	bone regeneration	210:226	Biocompatible polymers and drug-delivery scaffolds have driven development in bone regeneration.
33278972	0	2	theme	bone	113:116	arg1	regeneration					118:129	bone regeneration	113:129	bone regeneration	113:129	Chitosan-coated hydroxyapatite and drug-loaded polytrimethylene carbonate/polylactic acid scaffold for enhancing bone regeneration.
33278972	5	3	theme	microcomputer	947:959	arg1	scanning					972:979	microcomputer tomography scanning	947:979	microcomputer tomography scanning	947:979	The scaffolds were characterized via scanning electron microscopy, in vitro comprehensive performance testing, cell culturing, and microcomputer tomography scanning.
33278972	3	4	theme	PTMC	528:531	arg1	microspheres					533:544	PTMC microspheres	528:544	PTMC microspheres	528:544	The incorporation of PLLA, OA-HA, and VH into PTMC microspheres not only slowed the biodegradability of the scaffold but also enhanced its mechanical properties and surface properties.
33278972	8	5	theme	tissue	1264:1269	arg1	applications					1283:1294	bone tissue engineering applications	1259:1294	bone tissue engineering applications	1259:1294	Our findings suggest that the CS-PTMC/PLLA/OA-HA/VH microsphere scaffold is promising for bone tissue engineering applications.
33278972	2	6	theme	acid	326:329	arg1	VH					407:408	VH	407:408	VH	407:408	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	2	6	theme	acid	326:329	arg1	hydrochloride					392:404	chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride	260:404	a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release	258:447	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	4	7	theme	controlled	787:796	arg1	release					798:804	the controlled release	783:804	the controlled release of OA-HA	783:813	Moreover, the CS coating stimulated extensive adhesion of osteoblasts before OA-HA incorporation, which facilitated the controlled release of OA-HA.
33278972	3	8	theme	scaffold	590:597	arg1	biodegradability					566:581	the biodegradability	562:581	the biodegradability of the scaffold	562:597	The incorporation of PLLA, OA-HA, and VH into PTMC microspheres not only slowed the biodegradability of the scaffold but also enhanced its mechanical properties and surface properties.
33278972	2	9	theme	/polylactic	314:324	arg1	VH					407:408	VH	407:408	VH	407:408	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	2	9	theme	/polylactic	314:324	arg1	hydrochloride					392:404	chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride	260:404	a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release	258:447	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	2	10	theme	/vancomycin	380:390	arg1	VH					407:408	VH	407:408	VH	407:408	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	2	10	theme	/vancomycin	380:390	arg1	hydrochloride					392:404	chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride	260:404	a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release	258:447	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	2	11	theme	acid-modified	344:356	arg1	VH					407:408	VH	407:408	VH	407:408	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	2	11	theme	acid-modified	344:356	arg1	hydrochloride					392:404	chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride	260:404	a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release	258:447	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	1	12	theme	drug-delivery	159:171	arg1	scaffolds					173:181	drug-delivery scaffolds	159:181	drug-delivery scaffolds	159:181	Biocompatible polymers and drug-delivery scaffolds have driven development in bone regeneration.
33278972	5	13	theme	cell	927:930	arg1	culturing					932:940	cell culturing	927:940	cell culturing	927:940	The scaffolds were characterized via scanning electron microscopy, in vitro comprehensive performance testing, cell culturing, and microcomputer tomography scanning.
33278972	6	14	theme	scaffold	1050:1057	arg1	suitable					1063:1070	suitable	1063:1070	suitable	1063:1070	The results indicated that the surface of the composite microsphere scaffold was suitable for osteoblast adhesion.
33278972	6	14	theme	scaffold	1050:1057	arg1	surface					1013:1019	the surface	1009:1019	the surface of the composite microsphere scaffold	1009:1057	The results indicated that the surface of the composite microsphere scaffold was suitable for osteoblast adhesion.
33278972	5	15	theme	tomography	961:970	arg1	scanning					972:979	microcomputer tomography scanning	947:979	microcomputer tomography scanning	947:979	The scaffolds were characterized via scanning electron microscopy, in vitro comprehensive performance testing, cell culturing, and microcomputer tomography scanning.
33278972	2	16	theme	PTMC	309:312	arg1	VH					407:408	VH	407:408	VH	407:408	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	2	16	theme	PTMC	309:312	arg1	hydrochloride					392:404	chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride	260:404	a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release	258:447	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	2	17	theme	microsphere	411:421	arg1	scaffold					423:430	a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold	258:430	a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release	258:447	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	5	18	theme	scanning	853:860	arg1	microscopy					871:880	scanning electron microscopy	853:880	scanning electron microscopy	853:880	The scaffolds were characterized via scanning electron microscopy, in vitro comprehensive performance testing, cell culturing, and microcomputer tomography scanning.
33278972	2	19	theme	/oleic	337:342	arg1	VH					407:408	VH	407:408	VH	407:408	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	2	19	theme	/oleic	337:342	arg1	hydrochloride					392:404	chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride	260:404	a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release	258:447	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	4	20	theme	extensive	703:711	arg1	adhesion					713:720	extensive adhesion	703:720	extensive adhesion of osteoblasts	703:735	Moreover, the CS coating stimulated extensive adhesion of osteoblasts before OA-HA incorporation, which facilitated the controlled release of OA-HA.
33278972	0	21	theme	Chitosan-coated	0:14	arg1	hydroxyapatite					16:29	Chitosan-coated hydroxyapatite	0:29	Chitosan-coated hydroxyapatite	0:29	Chitosan-coated hydroxyapatite and drug-loaded polytrimethylene carbonate/polylactic acid scaffold for enhancing bone regeneration.
33278972	6	22	theme	microsphere	1038:1048	arg1	scaffold					1050:1057	the composite microsphere scaffold	1024:1057	the composite microsphere scaffold	1024:1057	The results indicated that the surface of the composite microsphere scaffold was suitable for osteoblast adhesion.
33278972	1	23	theme	Biocompatible	132:144	arg1	polymers					146:153	Biocompatible polymers	132:153	Biocompatible polymers	132:153	Biocompatible polymers and drug-delivery scaffolds have driven development in bone regeneration.
33278972	4	24	theme	OA-HA	809:813	arg1	release					798:804	the controlled release	783:804	the controlled release of OA-HA	783:813	Moreover, the CS coating stimulated extensive adhesion of osteoblasts before OA-HA incorporation, which facilitated the controlled release of OA-HA.
33278972	0	25	theme	drug-loaded	35:45	arg1	polytrimethylene					47:62	drug-loaded polytrimethylene	35:62	drug-loaded polytrimethylene	35:62	Chitosan-coated hydroxyapatite and drug-loaded polytrimethylene carbonate/polylactic acid scaffold for enhancing bone regeneration.
33278972	5	26	theme	electron	862:869	arg1	microscopy					871:880	scanning electron microscopy	853:880	scanning electron microscopy	853:880	The scaffolds were characterized via scanning electron microscopy, in vitro comprehensive performance testing, cell culturing, and microcomputer tomography scanning.
33278972	7	27	theme	osteogenic	1143:1152	arg1	proliferation					1154:1166	osteogenic proliferation	1143:1166	osteogenic proliferation	1143:1166	Additionally, the release of OA-HA stimulated osteogenic proliferation.
33278972	2	28	theme	PLLA	332:335	arg1	VH					407:408	VH	407:408	VH	407:408	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	2	28	theme	PLLA	332:335	arg1	hydrochloride					392:404	chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride	260:404	a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release	258:447	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	3	29	theme	PLLA	503:506	arg1	incorporation					486:498	The incorporation	482:498	The incorporation of PLLA, OA-HA, and VH into PTMC microspheres	482:544	The incorporation of PLLA, OA-HA, and VH into PTMC microspheres not only slowed the biodegradability of the scaffold but also enhanced its mechanical properties and surface properties.
33278972	2	30	theme	excellent	454:462	arg1	biocompatibility					464:479	excellent biocompatibility	454:479	excellent biocompatibility	454:479	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	4	31	theme	osteoblasts	725:735	arg1	adhesion					713:720	extensive adhesion	703:720	extensive adhesion of osteoblasts	703:735	Moreover, the CS coating stimulated extensive adhesion of osteoblasts before OA-HA incorporation, which facilitated the controlled release of OA-HA.
33278972	5	32	dep	in	883:884	arg1	vitro					886:890	vitro	886:890	vitro	886:890	The scaffolds were characterized via scanning electron microscopy, in vitro comprehensive performance testing, cell culturing, and microcomputer tomography scanning.
33278972	4	33	theme	OA-HA	744:748	arg1	incorporation					750:762	OA-HA incorporation	744:762	OA-HA incorporation	744:762	Moreover, the CS coating stimulated extensive adhesion of osteoblasts before OA-HA incorporation, which facilitated the controlled release of OA-HA.
33278972	7	34	theme	OA-HA	1126:1130	arg1	release					1115:1121	the release	1111:1121	the release of OA-HA	1111:1130	Additionally, the release of OA-HA stimulated osteogenic proliferation.
33278972	5	35	theme	comprehensive	892:904	arg1	testing					918:924	in vitro comprehensive performance testing	883:924	in vitro comprehensive performance testing	883:924	The scaffolds were characterized via scanning electron microscopy, in vitro comprehensive performance testing, cell culturing, and microcomputer tomography scanning.
33278972	2	36	theme	drug	436:439	arg1	release					441:447	drug release	436:447	drug release	436:447	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	2	37	theme	CS	270:271	arg1	VH					407:408	VH	407:408	VH	407:408	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	2	37	theme	CS	270:271	arg1	hydrochloride					392:404	chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride	260:404	a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release	258:447	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	5	38	theme	performance	906:916	arg1	testing					918:924	in vitro comprehensive performance testing	883:924	in vitro comprehensive performance testing	883:924	The scaffolds were characterized via scanning electron microscopy, in vitro comprehensive performance testing, cell culturing, and microcomputer tomography scanning.
33278972	8	39	theme	engineering	1271:1281	arg1	applications					1283:1294	bone tissue engineering applications	1259:1294	bone tissue engineering applications	1259:1294	Our findings suggest that the CS-PTMC/PLLA/OA-HA/VH microsphere scaffold is promising for bone tissue engineering applications.
33278972	6	40	theme	composite	1028:1036	arg1	scaffold					1050:1057	the composite microsphere scaffold	1024:1057	the composite microsphere scaffold	1024:1057	The results indicated that the surface of the composite microsphere scaffold was suitable for osteoblast adhesion.
33278972	2	41	theme	OA-HA	374:378	arg1	VH					407:408	VH	407:408	VH	407:408	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	2	41	theme	OA-HA	374:378	arg1	hydrochloride					392:404	chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride	260:404	a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release	258:447	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	0	42	theme	acid	85:88	arg1	scaffold					90:97	acid scaffold	85:97	acid scaffold	85:97	Chitosan-coated hydroxyapatite and drug-loaded polytrimethylene carbonate/polylactic acid scaffold for enhancing bone regeneration.
33278972	2	43	theme	chitosan	260:267	arg1	VH					407:408	VH	407:408	VH	407:408	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	2	43	theme	chitosan	260:267	arg1	hydrochloride					392:404	chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride	260:404	a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release	258:447	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	3	44	theme	VH	520:521	arg1	incorporation					486:498	The incorporation	482:498	The incorporation of PLLA, OA-HA, and VH into PTMC microspheres	482:544	The incorporation of PLLA, OA-HA, and VH into PTMC microspheres not only slowed the biodegradability of the scaffold but also enhanced its mechanical properties and surface properties.
33278972	8	45	theme	microsphere	1221:1231	arg1	promising					1245:1253	promising	1245:1253	promising	1245:1253	Our findings suggest that the CS-PTMC/PLLA/OA-HA/VH microsphere scaffold is promising for bone tissue engineering applications.
33278972	8	45	theme	microsphere	1221:1231	arg1	scaffold					1233:1240	the CS-PTMC/PLLA/OA-HA/VH microsphere scaffold	1195:1240	the CS-PTMC/PLLA/OA-HA/VH microsphere scaffold	1195:1240	Our findings suggest that the CS-PTMC/PLLA/OA-HA/VH microsphere scaffold is promising for bone tissue engineering applications.
33278972	8	46	theme	bone	1259:1262	arg1	applications					1283:1294	bone tissue engineering applications	1259:1294	bone tissue engineering applications	1259:1294	Our findings suggest that the CS-PTMC/PLLA/OA-HA/VH microsphere scaffold is promising for bone tissue engineering applications.
33278972	3	47	theme	surface	647:653	arg1	properties					655:664	surface properties	647:664	surface properties	647:664	The incorporation of PLLA, OA-HA, and VH into PTMC microspheres not only slowed the biodegradability of the scaffold but also enhanced its mechanical properties and surface properties.
33278972	3	48	theme	mechanical	621:630	arg1	properties					632:641	its mechanical properties	617:641	its mechanical properties	617:641	The incorporation of PLLA, OA-HA, and VH into PTMC microspheres not only slowed the biodegradability of the scaffold but also enhanced its mechanical properties and surface properties.
33278972	2	49	theme	hydroxyapatite	358:371	arg1	VH					407:408	VH	407:408	VH	407:408	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	2	49	theme	hydroxyapatite	358:371	arg1	hydrochloride					392:404	chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride	260:404	a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release	258:447	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	8	50	theme	CS-PTMC/PLLA/OA-HA/VH	1199:1219	arg1	promising					1245:1253	promising	1245:1253	promising	1245:1253	Our findings suggest that the CS-PTMC/PLLA/OA-HA/VH microsphere scaffold is promising for bone tissue engineering applications.
33278972	8	50	theme	CS-PTMC/PLLA/OA-HA/VH	1199:1219	arg1	scaffold					1233:1240	the CS-PTMC/PLLA/OA-HA/VH microsphere scaffold	1195:1240	the CS-PTMC/PLLA/OA-HA/VH microsphere scaffold	1195:1240	Our findings suggest that the CS-PTMC/PLLA/OA-HA/VH microsphere scaffold is promising for bone tissue engineering applications.
33278972	2	51	theme	carbonate	298:306	arg1	VH					407:408	VH	407:408	VH	407:408	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	2	51	theme	carbonate	298:306	arg1	hydrochloride					392:404	chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride	260:404	a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release	258:447	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	1	52	theme	driven	188:193	arg1	development					195:205	driven development	188:205	driven development	188:205	Biocompatible polymers and drug-delivery scaffolds have driven development in bone regeneration.
33278972	3	53	theme	OA-HA	509:513	arg1	incorporation					486:498	The incorporation	482:498	The incorporation of PLLA, OA-HA, and VH into PTMC microspheres	482:544	The incorporation of PLLA, OA-HA, and VH into PTMC microspheres not only slowed the biodegradability of the scaffold but also enhanced its mechanical properties and surface properties.
33278972	2	54	theme	polytrimethylene	281:296	arg1	VH					407:408	VH	407:408	VH	407:408	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	2	54	theme	polytrimethylene	281:296	arg1	hydrochloride					392:404	chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride	260:404	a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release	258:447	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	5	55	theme	in	883:884	arg1	testing					918:924	in vitro comprehensive performance testing	883:924	in vitro comprehensive performance testing	883:924	The scaffolds were characterized via scanning electron microscopy, in vitro comprehensive performance testing, cell culturing, and microcomputer tomography scanning.
33278972	4	56	theme	CS	681:682	arg1	coating					684:690	the CS coating	677:690	the CS coating	677:690	Moreover, the CS coating stimulated extensive adhesion of osteoblasts before OA-HA incorporation, which facilitated the controlled release of OA-HA.
33278972	2	57	theme	-coated	273:279	arg1	VH					407:408	VH	407:408	VH	407:408	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	2	57	theme	-coated	273:279	arg1	hydrochloride					392:404	chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride	260:404	a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release	258:447	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	2	58	theme	hydrochloride	392:404	arg1	scaffold					423:430	a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold	258:430	a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release	258:447	In this study, we fabricated a chitosan (CS)-coated polytrimethylene carbonate (PTMC)/polylactic acid (PLLA)/oleic acid-modified hydroxyapatite (OA-HA)/vancomycin hydrochloride (VH) microsphere scaffold for drug release with excellent biocompatibility.
33278972	6	59	theme	osteoblast	1076:1085	arg1	adhesion					1087:1094	osteoblast adhesion	1076:1094	osteoblast adhesion	1076:1094	The results indicated that the surface of the composite microsphere scaffold was suitable for osteoblast adhesion.
34568845	1	0	theme	tag	248:250	arg1	spectrometry					283:294	tandem mass tag multiplexing-based tandem mass spectrometry	236:294	tandem mass tag multiplexing-based tandem mass spectrometry	236:294	Here, we describe a fractionation protocol optimized to quantify changes in relative abundance of the chromatin-bound proteome (chromatome) by tandem mass tag multiplexing-based tandem mass spectrometry.
34568845	1	1	from	changes	158:164	arg1	abundance					178:186	relative abundance	169:186	relative abundance of the chromatin-bound proteome (chromatome)	169:231	Here, we describe a fractionation protocol optimized to quantify changes in relative abundance of the chromatin-bound proteome (chromatome) by tandem mass tag multiplexing-based tandem mass spectrometry.
34568845	3	2	theme	sample	520:525	arg1	preparation					527:537	sample preparation	520:537	sample preparation for mass spectrometry	520:559	We detail steps for stringent chromatin fractionation, sample preparation for mass spectrometry, and its evaluation.
34568845	1	3	theme	multiplexing-based	252:269	arg1	spectrometry					283:294	tandem mass tag multiplexing-based tandem mass spectrometry	236:294	tandem mass tag multiplexing-based tandem mass spectrometry	236:294	Here, we describe a fractionation protocol optimized to quantify changes in relative abundance of the chromatin-bound proteome (chromatome) by tandem mass tag multiplexing-based tandem mass spectrometry.
34568845	1	4	theme	tandem	271:276	arg1	spectrometry					283:294	tandem mass tag multiplexing-based tandem mass spectrometry	236:294	tandem mass tag multiplexing-based tandem mass spectrometry	236:294	Here, we describe a fractionation protocol optimized to quantify changes in relative abundance of the chromatin-bound proteome (chromatome) by tandem mass tag multiplexing-based tandem mass spectrometry.
34568845	1	5	theme	mass	278:281	arg1	spectrometry					283:294	tandem mass tag multiplexing-based tandem mass spectrometry	236:294	tandem mass tag multiplexing-based tandem mass spectrometry	236:294	Here, we describe a fractionation protocol optimized to quantify changes in relative abundance of the chromatin-bound proteome (chromatome) by tandem mass tag multiplexing-based tandem mass spectrometry.
34568845	4	6	from	details	595:601	arg1	execution					618:626	execution	618:626	execution	618:626	For complete details on the use and execution of this protocol, please refer to Challa et al. (2021).
34568845	4	6	from	details	595:601	arg1	use					610:612	use	610:612	use	610:612	For complete details on the use and execution of this protocol, please refer to Challa et al. (2021).
34568845	3	7	theme	mass	543:546	arg1	spectrometry					548:559	mass spectrometry	543:559	mass spectrometry	543:559	We detail steps for stringent chromatin fractionation, sample preparation for mass spectrometry, and its evaluation.
34568845	1	8	theme	relative	169:176	arg1	abundance					178:186	relative abundance	169:186	relative abundance of the chromatin-bound proteome (chromatome)	169:231	Here, we describe a fractionation protocol optimized to quantify changes in relative abundance of the chromatin-bound proteome (chromatome) by tandem mass tag multiplexing-based tandem mass spectrometry.
34568845	0	9	theme	Sucrose	0:6	arg1	enrichment					27:36	Sucrose gradient chromatin enrichment	0:36	Sucrose gradient chromatin enrichment for quantitative proteomics analysis in budding yeast.	0:91	Sucrose gradient chromatin enrichment for quantitative proteomics analysis in budding yeast.
34568845	0	10	theme	gradient	8:15	arg1	enrichment					27:36	Sucrose gradient chromatin enrichment	0:36	Sucrose gradient chromatin enrichment for quantitative proteomics analysis in budding yeast.	0:91	Sucrose gradient chromatin enrichment for quantitative proteomics analysis in budding yeast.
34568845	2	11	theme	yeast	320:324	arg1	cells					326:330	yeast cells	320:330	yeast cells	320:330	It has been applied to yeast cells before and after exposure to DNA-damaging drugs to characterize changes in chromatin composition induced by the DNA damage response.
34568845	2	12	theme	damage	448:453	arg1	response					455:462	the DNA damage response	440:462	the DNA damage response	440:462	It has been applied to yeast cells before and after exposure to DNA-damaging drugs to characterize changes in chromatin composition induced by the DNA damage response.
34568845	2	13	theme	DNA	444:446	arg1	damage					448:453	DNA damage	444:453	the DNA damage response	440:462	It has been applied to yeast cells before and after exposure to DNA-damaging drugs to characterize changes in chromatin composition induced by the DNA damage response.
34568845	1	14	theme	chromatin-bound	195:209	arg1	proteome					211:218	the chromatin-bound proteome	191:218	the chromatin-bound proteome (chromatome)	191:231	Here, we describe a fractionation protocol optimized to quantify changes in relative abundance of the chromatin-bound proteome (chromatome) by tandem mass tag multiplexing-based tandem mass spectrometry.
34568845	1	14	theme	chromatin-bound	195:209	arg1	chromatome					221:230	chromatome	221:230	chromatome	221:230	Here, we describe a fractionation protocol optimized to quantify changes in relative abundance of the chromatin-bound proteome (chromatome) by tandem mass tag multiplexing-based tandem mass spectrometry.
34568845	0	15	theme	chromatin	17:25	arg1	enrichment					27:36	Sucrose gradient chromatin enrichment	0:36	Sucrose gradient chromatin enrichment for quantitative proteomics analysis in budding yeast.	0:91	Sucrose gradient chromatin enrichment for quantitative proteomics analysis in budding yeast.
34568845	1	16	theme	proteome	211:218	arg1	abundance					178:186	relative abundance	169:186	relative abundance of the chromatin-bound proteome (chromatome)	169:231	Here, we describe a fractionation protocol optimized to quantify changes in relative abundance of the chromatin-bound proteome (chromatome) by tandem mass tag multiplexing-based tandem mass spectrometry.
34568845	0	17	theme	quantitative	42:53	arg1	analysis					66:73	quantitative proteomics analysis	42:73	quantitative proteomics analysis	42:73	Sucrose gradient chromatin enrichment for quantitative proteomics analysis in budding yeast.
34568845	4	18	theme	complete	586:593	arg1	details					595:601	complete details	586:601	complete details on the use and execution of this protocol	586:643	For complete details on the use and execution of this protocol, please refer to Challa et al. (2021).
34568845	2	19	theme	chromatin	407:415	arg1	composition					417:427	chromatin composition	407:427	chromatin composition induced by the DNA damage response	407:462	It has been applied to yeast cells before and after exposure to DNA-damaging drugs to characterize changes in chromatin composition induced by the DNA damage response.
34568845	3	20	theme	chromatin	495:503	arg1	fractionation					505:517	stringent chromatin fractionation	485:517	stringent chromatin fractionation	485:517	We detail steps for stringent chromatin fractionation, sample preparation for mass spectrometry, and its evaluation.
34568845	0	21	theme	proteomics	55:64	arg1	analysis					66:73	quantitative proteomics analysis	42:73	quantitative proteomics analysis	42:73	Sucrose gradient chromatin enrichment for quantitative proteomics analysis in budding yeast.
34568845	1	22	theme	fractionation	113:125	arg1	protocol					127:134	a fractionation protocol	111:134	a fractionation protocol optimized to quantify changes in relative abundance of the chromatin-bound proteome (chromatome) by tandem mass tag multiplexing-based tandem mass spectrometry	111:294	Here, we describe a fractionation protocol optimized to quantify changes in relative abundance of the chromatin-bound proteome (chromatome) by tandem mass tag multiplexing-based tandem mass spectrometry.
34568845	2	23	from	changes	396:402	arg1	composition					417:427	chromatin composition	407:427	chromatin composition induced by the DNA damage response	407:462	It has been applied to yeast cells before and after exposure to DNA-damaging drugs to characterize changes in chromatin composition induced by the DNA damage response.
34568845	3	24	theme	stringent	485:493	arg1	fractionation					505:517	stringent chromatin fractionation	485:517	stringent chromatin fractionation	485:517	We detail steps for stringent chromatin fractionation, sample preparation for mass spectrometry, and its evaluation.
34568845	0	25	theme	budding	78:84	arg1	yeast					86:90	budding yeast	78:90	budding yeast	78:90	Sucrose gradient chromatin enrichment for quantitative proteomics analysis in budding yeast.
34568845	4	26	dep	use	610:612	arg1	the					606:608	the	606:608	the	606:608	For complete details on the use and execution of this protocol, please refer to Challa et al. (2021).
34568845	4	27	theme	protocol	636:643	arg1	execution					618:626	execution	618:626	execution	618:626	For complete details on the use and execution of this protocol, please refer to Challa et al. (2021).
34568845	4	27	theme	protocol	636:643	arg1	use					610:612	use	610:612	use	610:612	For complete details on the use and execution of this protocol, please refer to Challa et al. (2021).
34568845	1	28	theme	tandem	236:241	arg1	spectrometry					283:294	tandem mass tag multiplexing-based tandem mass spectrometry	236:294	tandem mass tag multiplexing-based tandem mass spectrometry	236:294	Here, we describe a fractionation protocol optimized to quantify changes in relative abundance of the chromatin-bound proteome (chromatome) by tandem mass tag multiplexing-based tandem mass spectrometry.
34568845	0	29	from	enrichment	27:36	arg1	yeast					86:90	budding yeast	78:90	budding yeast	78:90	Sucrose gradient chromatin enrichment for quantitative proteomics analysis in budding yeast.
34568845	2	30	theme	DNA-damaging	361:372	arg1	drugs					374:378	DNA-damaging drugs	361:378	DNA-damaging drugs	361:378	It has been applied to yeast cells before and after exposure to DNA-damaging drugs to characterize changes in chromatin composition induced by the DNA damage response.
34568845	1	31	theme	mass	243:246	arg1	spectrometry					283:294	tandem mass tag multiplexing-based tandem mass spectrometry	236:294	tandem mass tag multiplexing-based tandem mass spectrometry	236:294	Here, we describe a fractionation protocol optimized to quantify changes in relative abundance of the chromatin-bound proteome (chromatome) by tandem mass tag multiplexing-based tandem mass spectrometry.
32800353	11	0	theme	antibacterial	1737:1749	arg1	group					1770:1774	antibacterial and remineralizing group	1737:1774	antibacterial and remineralizing group (P < 0.05)	1737:1785	Under biofilm acids, enamel hardness at the margins was decreased to about 0.5 GPa for control; it was about 1 GPa for antibacterial group, and 1.3 GPa for antibacterial and remineralizing group (P < 0.05).
32800353	11	0	theme	antibacterial	1737:1749	arg1	<					1779:1779	P < 0.05	1777:1784	P < 0.05	1777:1784	Under biofilm acids, enamel hardness at the margins was decreased to about 0.5 GPa for control; it was about 1 GPa for antibacterial group, and 1.3 GPa for antibacterial and remineralizing group (P < 0.05).
32800353	2	1	theme	antibacterial	341:353	arg1	function					355:362	antibacterial function	341:362	antibacterial function	341:362	The objectives of this study were to: (1) develop a novel multifunctional composite with antibacterial function and calcium (Ca) and phosphate (P) ion release, and (2) investigate the effects on enamel demineralization and hardness at the margins under biofilms.
32800353	11	2	theme	remineralizing	1755:1768	arg1	group					1770:1774	antibacterial and remineralizing group	1737:1774	antibacterial and remineralizing group (P < 0.05)	1737:1785	Under biofilm acids, enamel hardness at the margins was decreased to about 0.5 GPa for control; it was about 1 GPa for antibacterial group, and 1.3 GPa for antibacterial and remineralizing group (P < 0.05).
32800353	11	2	theme	remineralizing	1755:1768	arg1	<					1779:1779	P < 0.05	1777:1784	P < 0.05	1777:1784	Under biofilm acids, enamel hardness at the margins was decreased to about 0.5 GPa for control; it was about 1 GPa for antibacterial group, and 1.3 GPa for antibacterial and remineralizing group (P < 0.05).
32800353	6	3	theme	lactic	972:977	arg1	units					959:963	Colony-forming units	944:963	Colony-forming units (CFU)	944:969	Colony-forming units (CFU), lactic acid and polysaccharide of Streptococcus mutans (S. mutans) biofilms were evaluated.
32800353	6	3	theme	lactic	972:977	arg1	acid					979:982	lactic acid	972:982	lactic acid	972:982	Colony-forming units (CFU), lactic acid and polysaccharide of Streptococcus mutans (S. mutans) biofilms were evaluated.
32800353	4	4	theme	%	873:873	arg1	NACP					875:878	30% NACP	871:878	30% NACP	871:878	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	12	5	theme	NACP	1826:1829	arg1	composite					1831:1839	30% NACP composite	1822:1839	30% NACP composite	1822:1839	CONCLUSIONS The novel 3% DMAHDM + 30% NACP composite had strong antibacterial effects.
32800353	4	6	dep	antibacterial	819:831	arg1	D					816:816	D	816:816	D	816:816	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	14	7	theme	recurrent	2119:2127	arg1	caries					2129:2134	recurrent caries	2119:2134	recurrent caries	2119:2134	The novel multifunctional composite is promising to inhibit recurrent caries.
32800353	6	8	theme	biofilms	1039:1046	arg1	units					959:963	Colony-forming units	944:963	Colony-forming units (CFU)	944:969	Colony-forming units (CFU), lactic acid and polysaccharide of Streptococcus mutans (S. mutans) biofilms were evaluated.
32800353	6	8	theme	biofilms	1039:1046	arg1	polysaccharide					988:1001	polysaccharide	988:1001	polysaccharide	988:1001	Colony-forming units (CFU), lactic acid and polysaccharide of Streptococcus mutans (S. mutans) biofilms were evaluated.
32800353	6	8	theme	biofilms	1039:1046	arg1	acid					979:982	lactic acid	972:982	lactic acid	972:982	Colony-forming units (CFU), lactic acid and polysaccharide of Streptococcus mutans (S. mutans) biofilms were evaluated.
32800353	6	8	theme	biofilms	1039:1046	arg1	CFU					966:968	CFU	966:968	CFU	966:968	Colony-forming units (CFU), lactic acid and polysaccharide of Streptococcus mutans (S. mutans) biofilms were evaluated.
32800353	10	9	theme	lactic	1472:1477	arg1	acid					1479:1482	Biofilm lactic acid	1464:1482	cariogenic low pH. Biofilm lactic acid	1445:1482	Ca and P ion release of 3% DMAHDM + 30% NACP was increased at cariogenic low pH. Biofilm lactic acid and polysaccharides were greatly decreased via DMAHDM, and CFU was reduced by 4 logs (P < 0.05).
32800353	1	10	theme	primary	188:194	arg1	reason					196:201	a primary reason	186:201	a primary reason for restoration failure caused by biofilm acids	186:249	OBJECTIVE Recurrent caries is a primary reason for restoration failure caused by biofilm acids.
32800353	1	10	theme	primary	188:194	arg1	caries					176:181	OBJECTIVE Recurrent caries	156:181	OBJECTIVE Recurrent caries	156:181	OBJECTIVE Recurrent caries is a primary reason for restoration failure caused by biofilm acids.
32800353	11	11	theme	antibacterial	1700:1712	arg1	group					1714:1718	antibacterial group	1700:1718	antibacterial group	1700:1718	Under biofilm acids, enamel hardness at the margins was decreased to about 0.5 GPa for control; it was about 1 GPa for antibacterial group, and 1.3 GPa for antibacterial and remineralizing group (P < 0.05).
32800353	12	12	dep	CONCLUSIONS	1788:1798	arg1	composite					1831:1839	30% NACP composite	1822:1839	30% NACP composite	1822:1839	CONCLUSIONS The novel 3% DMAHDM + 30% NACP composite had strong antibacterial effects.
32800353	12	12	dep	CONCLUSIONS	1788:1798	arg1	DMAHDM					1813:1818	The novel 3% DMAHDM	1800:1818	The novel 3% DMAHDM	1800:1818	CONCLUSIONS The novel 3% DMAHDM + 30% NACP composite had strong antibacterial effects.
32800353	4	13	theme	%	794:794	arg1	DMAHDM					796:801	3% DMAHDM	793:801	3% DMAHDM	793:801	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	6	14	theme	Colony-forming	944:957	arg1	units					959:963	Colony-forming units	944:963	Colony-forming units (CFU)	944:969	Colony-forming units (CFU), lactic acid and polysaccharide of Streptococcus mutans (S. mutans) biofilms were evaluated.
32800353	6	14	theme	Colony-forming	944:957	arg1	polysaccharide					988:1001	polysaccharide	988:1001	polysaccharide	988:1001	Colony-forming units (CFU), lactic acid and polysaccharide of Streptococcus mutans (S. mutans) biofilms were evaluated.
32800353	6	14	theme	Colony-forming	944:957	arg1	acid					979:982	lactic acid	972:982	lactic acid	972:982	Colony-forming units (CFU), lactic acid and polysaccharide of Streptococcus mutans (S. mutans) biofilms were evaluated.
32800353	6	14	theme	Colony-forming	944:957	arg1	CFU					966:968	CFU	966:968	CFU	966:968	Colony-forming units (CFU), lactic acid and polysaccharide of Streptococcus mutans (S. mutans) biofilms were evaluated.
32800353	0	15	theme	bovine	121:126	arg1	margins					147:153	bovine enamel-restoration margins	121:153	bovine enamel-restoration margins	121:153	In vitro evaluation of composite containing DMAHDM and calcium phosphate nanoparticles on recurrent caries inhibition at bovine enamel-restoration margins.
32800353	10	16	theme	cariogenic	1445:1454	arg1	acid					1479:1482	Biofilm lactic acid	1464:1482	cariogenic low pH. Biofilm lactic acid	1445:1482	Ca and P ion release of 3% DMAHDM + 30% NACP was increased at cariogenic low pH. Biofilm lactic acid and polysaccharides were greatly decreased via DMAHDM, and CFU was reduced by 4 logs (P < 0.05).
32800353	13	17	theme	biofilm	1955:1961	arg1	attacks					1968:1974	biofilm acid attacks	1955:1974	biofilm acid attacks	1955:1974	It substantially reduced enamel demineralization adjacent to restorations under biofilm acid attacks, yielding enamel hardness that was 2-fold greater than that of control composites.
32800353	13	18	theme	adjacent	1924:1931	arg1	demineralization					1907:1922	enamel demineralization	1900:1922	enamel demineralization adjacent to restorations under biofilm acid attacks	1900:1974	It substantially reduced enamel demineralization adjacent to restorations under biofilm acid attacks, yielding enamel hardness that was 2-fold greater than that of control composites.
32800353	10	19	theme	P	1390:1390	arg1	release					1396:1402	Ca and P ion release	1383:1402	release	1396:1402	Ca and P ion release of 3% DMAHDM + 30% NACP was increased at cariogenic low pH. Biofilm lactic acid and polysaccharides were greatly decreased via DMAHDM, and CFU was reduced by 4 logs (P < 0.05).
32800353	5	20	theme	Mechanical	882:891	arg1	properties					893:902	Mechanical properties	882:902	Mechanical properties	882:902	Mechanical properties and Ca and P ion release were measured.
32800353	4	21	theme	antibacterial	772:784	arg1	group					786:790	(3) antibacterial group	768:790	(3) antibacterial group (3% DMAHDM + 0% NACP)	768:812	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	6	22	theme	mutans	1020:1025	arg1	biofilms					1039:1046	Streptococcus mutans (S. mutans) biofilms	1006:1046	Streptococcus mutans (S. mutans) biofilms	1006:1046	Colony-forming units (CFU), lactic acid and polysaccharide of Streptococcus mutans (S. mutans) biofilms were evaluated.
32800353	10	23	theme	Ca	1383:1384	arg1	release					1396:1402	Ca and P ion release	1383:1402	release	1396:1402	Ca and P ion release of 3% DMAHDM + 30% NACP was increased at cariogenic low pH. Biofilm lactic acid and polysaccharides were greatly decreased via DMAHDM, and CFU was reduced by 4 logs (P < 0.05).
32800353	4	24	dep	control	698:704	arg1	1					684:684	1	684:684	1	684:684	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	2	25	theme	study	275:279	arg1	to					286:287	to	286:287	to	286:287	The objectives of this study were to: (1) develop a novel multifunctional composite with antibacterial function and calcium (Ca) and phosphate (P) ion release, and (2) investigate the effects on enamel demineralization and hardness at the margins under biofilms.
32800353	2	25	theme	study	275:279	arg1	objectives					256:265	The objectives	252:265	The objectives of this study	252:279	The objectives of this study were to: (1) develop a novel multifunctional composite with antibacterial function and calcium (Ca) and phosphate (P) ion release, and (2) investigate the effects on enamel demineralization and hardness at the margins under biofilms.
32800353	11	26	from	margins	1625:1631	arg1	hardness					1609:1616	enamel hardness	1602:1616	enamel hardness at the margins	1602:1631	Under biofilm acids, enamel hardness at the margins was decreased to about 0.5 GPa for control; it was about 1 GPa for antibacterial group, and 1.3 GPa for antibacterial and remineralizing group (P < 0.05).
32800353	3	27	theme	calcium	599:605	arg1	NACP					618:621	NACP	618:621	NACP	618:621	METHODS Dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP) were incorporated into composite.
32800353	3	27	theme	calcium	599:605	arg1	phosphate					607:615	amorphous calcium phosphate	589:615	amorphous calcium phosphate (NACP)	589:622	METHODS Dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP) were incorporated into composite.
32800353	2	28	theme	enamel	447:452	arg1	demineralization					454:469	enamel demineralization	447:469	enamel demineralization	447:469	The objectives of this study were to: (1) develop a novel multifunctional composite with antibacterial function and calcium (Ca) and phosphate (P) ion release, and (2) investigate the effects on enamel demineralization and hardness at the margins under biofilms.
32800353	8	29	theme	comparison	1262:1271	arg1	tests					1273:1277	Tukey's multiple comparison tests	1245:1277	Tukey's multiple comparison tests	1245:1277	Data were analyzed via one-way and two-way analyses of variance and Tukey's multiple comparison tests.
32800353	6	30	dep	mutans	1020:1025	arg1	mutans					1031:1036	S. mutans	1028:1036	S. mutans	1028:1036	Colony-forming units (CFU), lactic acid and polysaccharide of Streptococcus mutans (S. mutans) biofilms were evaluated.
32800353	4	31	dep	group	786:790	arg1	NACP					808:811	0% NACP	805:811	0% NACP	805:811	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	4	31	dep	group	786:790	arg1	DMAHDM					796:801	3% DMAHDM	793:801	3% DMAHDM	793:801	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	4	31	dep	group	786:790	arg1	3					769:769	3	769:769	3	769:769	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	5	32	theme	ion	917:919	arg1	release					921:927	Ca and P ion release	908:927	Ca and P ion release	908:927	Mechanical properties and Ca and P ion release were measured.
32800353	4	33	theme	%	860:860	arg1	DMAHDM					862:867	3% DMAHDM	859:867	3% DMAHDM	859:867	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	3	34	dep	METHODS	515:521	arg1	DMAHDM					560:565	DMAHDM	560:565	DMAHDM	560:565	METHODS Dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP) were incorporated into composite.
32800353	3	34	dep	METHODS	515:521	arg1	nanoparticles					572:584	nanoparticles	572:584	nanoparticles of amorphous calcium phosphate (NACP)	572:622	METHODS Dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP) were incorporated into composite.
32800353	3	34	dep	METHODS	515:521	arg1	methacrylate					546:557	Dimethylaminohexadecyl methacrylate	523:557	Dimethylaminohexadecyl methacrylate (DMAHDM)	523:566	METHODS Dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP) were incorporated into composite.
32800353	13	35	theme	enamel	1900:1905	arg1	demineralization					1907:1922	enamel demineralization	1900:1922	enamel demineralization adjacent to restorations under biofilm acid attacks	1900:1974	It substantially reduced enamel demineralization adjacent to restorations under biofilm acid attacks, yielding enamel hardness that was 2-fold greater than that of control composites.
32800353	4	36	theme	%	806:806	arg1	NACP					808:811	0% NACP	805:811	0% NACP	805:811	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	12	37	theme	antibacterial	1852:1864	arg1	effects					1866:1872	strong antibacterial effects	1845:1872	strong antibacterial effects	1845:1872	CONCLUSIONS The novel 3% DMAHDM + 30% NACP composite had strong antibacterial effects.
32800353	4	38	dep	tested	675:680	arg1	control					737:743	(2) Experimental control	720:743	(2) Experimental control (0% DMAHDM + 0% NACP)	720:765	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	4	38	dep	tested	675:680	arg1	Heliomolar					707:716	Heliomolar	707:716	Heliomolar	707:716	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	4	38	dep	tested	675:680	arg1	group					786:790	(3) antibacterial group	768:790	(3) antibacterial group (3% DMAHDM + 0% NACP)	768:812	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	4	38	dep	tested	675:680	arg1	group					852:856	remineralizing group	837:856	remineralizing group (3% DMAHDM + 30% NACP)	837:879	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	4	38	dep	tested	675:680	arg1	control					698:704	(1) Commercial control	683:704	(1) Commercial control (Heliomolar)	683:717	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	4	38	dep	tested	675:680	arg1	antibacterial					819:831	(D) antibacterial	815:831	(D) antibacterial	815:831	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	0	39	theme	recurrent	90:98	arg1	inhibition					107:116	recurrent caries inhibition	90:116	recurrent caries inhibition at bovine enamel-restoration margins	90:153	In vitro evaluation of composite containing DMAHDM and calcium phosphate nanoparticles on recurrent caries inhibition at bovine enamel-restoration margins.
32800353	12	40	theme	novel	1804:1808	arg1	DMAHDM					1813:1818	The novel 3% DMAHDM	1800:1818	The novel 3% DMAHDM	1800:1818	CONCLUSIONS The novel 3% DMAHDM + 30% NACP composite had strong antibacterial effects.
32800353	10	41	theme	%	1408:1408	arg1	DMAHDM					1410:1415	3% DMAHDM	1407:1415	3% DMAHDM	1407:1415	Ca and P ion release of 3% DMAHDM + 30% NACP was increased at cariogenic low pH. Biofilm lactic acid and polysaccharides were greatly decreased via DMAHDM, and CFU was reduced by 4 logs (P < 0.05).
32800353	4	42	theme	3	859:859	arg1	%					860:860	%	860:860	%	860:860	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	4	43	theme	Commercial	687:696	arg1	control					698:704	(1) Commercial control	683:704	(1) Commercial control (Heliomolar)	683:717	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	4	43	theme	Commercial	687:696	arg1	Heliomolar					707:716	Heliomolar	707:716	Heliomolar	707:716	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	11	44	theme	biofilm	1587:1593	arg1	acids					1595:1599	biofilm acids	1587:1599	biofilm acids	1587:1599	Under biofilm acids, enamel hardness at the margins was decreased to about 0.5 GPa for control; it was about 1 GPa for antibacterial group, and 1.3 GPa for antibacterial and remineralizing group (P < 0.05).
32800353	0	45	theme	In	0:1	arg1	evaluation					9:18	In vitro evaluation	0:18	In vitro evaluation of composite containing DMAHDM and calcium phosphate	0:71	In vitro evaluation of composite containing DMAHDM and calcium phosphate nanoparticles on recurrent caries inhibition at bovine enamel-restoration margins.
32800353	10	46	theme	%	1421:1421	arg1	NACP					1423:1426	30% NACP	1419:1426	30% NACP	1419:1426	Ca and P ion release of 3% DMAHDM + 30% NACP was increased at cariogenic low pH. Biofilm lactic acid and polysaccharides were greatly decreased via DMAHDM, and CFU was reduced by 4 logs (P < 0.05).
32800353	9	47	theme	P	1372:1372	arg1	properties					1360:1369	the mechanical properties	1345:1369	the mechanical properties (P > 0.05)	1345:1380	RESULTS Adding DMAHDM and NACP into composite did not compromise the mechanical properties (P > 0.05).
32800353	9	47	theme	P	1372:1372	arg1	>					1374:1374	P > 0.05	1372:1379	P > 0.05	1372:1379	RESULTS Adding DMAHDM and NACP into composite did not compromise the mechanical properties (P > 0.05).
32800353	10	48	dep	acid	1479:1482	arg1	pH.					1460:1462	cariogenic low pH. Biofilm lactic acid	1445:1482	cariogenic low pH. Biofilm lactic acid	1445:1482	Ca and P ion release of 3% DMAHDM + 30% NACP was increased at cariogenic low pH. Biofilm lactic acid and polysaccharides were greatly decreased via DMAHDM, and CFU was reduced by 4 logs (P < 0.05).
32800353	10	49	theme	P	1570:1570	arg1	logs					1564:1567	4 logs	1562:1567	4 logs (P < 0.05)	1562:1578	Ca and P ion release of 3% DMAHDM + 30% NACP was increased at cariogenic low pH. Biofilm lactic acid and polysaccharides were greatly decreased via DMAHDM, and CFU was reduced by 4 logs (P < 0.05).
32800353	10	49	theme	P	1570:1570	arg1	<					1572:1572	P < 0.05	1570:1577	P < 0.05	1570:1577	Ca and P ion release of 3% DMAHDM + 30% NACP was increased at cariogenic low pH. Biofilm lactic acid and polysaccharides were greatly decreased via DMAHDM, and CFU was reduced by 4 logs (P < 0.05).
32800353	0	50	theme	composite	23:31	arg1	DMAHDM					44:49	composite containing DMAHDM	23:49	composite containing DMAHDM	23:49	In vitro evaluation of composite containing DMAHDM and calcium phosphate nanoparticles on recurrent caries inhibition at bovine enamel-restoration margins.
32800353	7	51	theme	enamel	1091:1096	arg1	Demineralization					1064:1079	Demineralization	1064:1079	Demineralization of bovine enamel with restorations	1064:1114	Demineralization of bovine enamel with restorations was induced via S. mutans, and enamel hardness was measured.
32800353	0	52	theme	calcium	55:61	arg1	phosphate					63:71	calcium phosphate	55:71	calcium phosphate	55:71	In vitro evaluation of composite containing DMAHDM and calcium phosphate nanoparticles on recurrent caries inhibition at bovine enamel-restoration margins.
32800353	12	53	theme	30	1822:1823	arg1	%					1824:1824	%	1824:1824	%	1824:1824	CONCLUSIONS The novel 3% DMAHDM + 30% NACP composite had strong antibacterial effects.
32800353	0	54	theme	DMAHDM	44:49	arg1	evaluation					9:18	In vitro evaluation	0:18	In vitro evaluation of composite containing DMAHDM and calcium phosphate	0:71	In vitro evaluation of composite containing DMAHDM and calcium phosphate nanoparticles on recurrent caries inhibition at bovine enamel-restoration margins.
32800353	13	55	theme	control	2039:2045	arg1	composites					2047:2056	control composites	2039:2056	control composites	2039:2056	It substantially reduced enamel demineralization adjacent to restorations under biofilm acid attacks, yielding enamel hardness that was 2-fold greater than that of control composites.
32800353	14	56	theme	multifunctional	2069:2083	arg1	composite					2085:2093	The novel multifunctional composite	2059:2093	The novel multifunctional composite	2059:2093	The novel multifunctional composite is promising to inhibit recurrent caries.
32800353	14	56	theme	multifunctional	2069:2083	arg1	promising					2098:2106	promising	2098:2106	promising	2098:2106	The novel multifunctional composite is promising to inhibit recurrent caries.
32800353	2	57	theme	multifunctional	310:324	arg1	composite					326:334	a novel multifunctional composite	302:334	a novel multifunctional composite with antibacterial function and calcium (Ca) and phosphate (P) ion release	302:409	The objectives of this study were to: (1) develop a novel multifunctional composite with antibacterial function and calcium (Ca) and phosphate (P) ion release, and (2) investigate the effects on enamel demineralization and hardness at the margins under biofilms.
32800353	1	58	theme	biofilm	237:243	arg1	acids					245:249	biofilm acids	237:249	biofilm acids	237:249	OBJECTIVE Recurrent caries is a primary reason for restoration failure caused by biofilm acids.
32800353	1	59	theme	Recurrent	166:174	arg1	reason					196:201	a primary reason	186:201	a primary reason for restoration failure caused by biofilm acids	186:249	OBJECTIVE Recurrent caries is a primary reason for restoration failure caused by biofilm acids.
32800353	1	59	theme	Recurrent	166:174	arg1	caries					176:181	OBJECTIVE Recurrent caries	156:181	OBJECTIVE Recurrent caries	156:181	OBJECTIVE Recurrent caries is a primary reason for restoration failure caused by biofilm acids.
32800353	0	60	from	margins	147:153	arg1	inhibition					107:116	recurrent caries inhibition	90:116	recurrent caries inhibition at bovine enamel-restoration margins	90:153	In vitro evaluation of composite containing DMAHDM and calcium phosphate nanoparticles on recurrent caries inhibition at bovine enamel-restoration margins.
32800353	10	61	theme	DMAHDM	1410:1415	arg1	release					1396:1402	Ca and P ion release	1383:1402	release	1396:1402	Ca and P ion release of 3% DMAHDM + 30% NACP was increased at cariogenic low pH. Biofilm lactic acid and polysaccharides were greatly decreased via DMAHDM, and CFU was reduced by 4 logs (P < 0.05).
32800353	12	62	theme	3	1810:1810	arg1	%					1811:1811	%	1811:1811	%	1811:1811	CONCLUSIONS The novel 3% DMAHDM + 30% NACP composite had strong antibacterial effects.
32800353	10	63	theme	Biofilm	1464:1470	arg1	acid					1479:1482	Biofilm lactic acid	1464:1482	cariogenic low pH. Biofilm lactic acid	1445:1482	Ca and P ion release of 3% DMAHDM + 30% NACP was increased at cariogenic low pH. Biofilm lactic acid and polysaccharides were greatly decreased via DMAHDM, and CFU was reduced by 4 logs (P < 0.05).
32800353	0	64	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro evaluation of composite containing DMAHDM and calcium phosphate nanoparticles on recurrent caries inhibition at bovine enamel-restoration margins.
32800353	4	65	theme	30	871:872	arg1	%					873:873	%	873:873	%	873:873	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	4	66	dep	group	852:856	arg1	NACP					875:878	30% NACP	871:878	30% NACP	871:878	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	4	66	dep	group	852:856	arg1	DMAHDM					862:867	3% DMAHDM	859:867	3% DMAHDM	859:867	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	10	67	theme	low	1456:1458	arg1	acid					1479:1482	Biofilm lactic acid	1464:1482	cariogenic low pH. Biofilm lactic acid	1445:1482	Ca and P ion release of 3% DMAHDM + 30% NACP was increased at cariogenic low pH. Biofilm lactic acid and polysaccharides were greatly decreased via DMAHDM, and CFU was reduced by 4 logs (P < 0.05).
32800353	2	68	theme	calcium	368:374	arg1	release					403:409	calcium (Ca) and phosphate (P) ion release	368:409	calcium (Ca) and phosphate (P) ion release	368:409	The objectives of this study were to: (1) develop a novel multifunctional composite with antibacterial function and calcium (Ca) and phosphate (P) ion release, and (2) investigate the effects on enamel demineralization and hardness at the margins under biofilms.
32800353	11	69	theme	P	1777:1777	arg1	group					1770:1774	antibacterial and remineralizing group	1737:1774	antibacterial and remineralizing group (P < 0.05)	1737:1785	Under biofilm acids, enamel hardness at the margins was decreased to about 0.5 GPa for control; it was about 1 GPa for antibacterial group, and 1.3 GPa for antibacterial and remineralizing group (P < 0.05).
32800353	11	69	theme	P	1777:1777	arg1	<					1779:1779	P < 0.05	1777:1784	P < 0.05	1777:1784	Under biofilm acids, enamel hardness at the margins was decreased to about 0.5 GPa for control; it was about 1 GPa for antibacterial group, and 1.3 GPa for antibacterial and remineralizing group (P < 0.05).
32800353	4	70	dep	control	737:743	arg1	2					721:721	2	721:721	2	721:721	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	4	70	dep	control	737:743	arg1	NACP					761:764	0% DMAHDM + 0% NACP	746:764	NACP	761:764	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	4	70	dep	control	737:743	arg1	DMAHDM					749:754	0% DMAHDM + 0% NACP	746:764	DMAHDM	749:754	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	7	71	with	Demineralization	1064:1079	arg1	restorations					1103:1114	restorations	1103:1114	restorations	1103:1114	Demineralization of bovine enamel with restorations was induced via S. mutans, and enamel hardness was measured.
32800353	3	72	theme	Dimethylaminohexadecyl	523:544	arg1	DMAHDM					560:565	DMAHDM	560:565	DMAHDM	560:565	METHODS Dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP) were incorporated into composite.
32800353	3	72	theme	Dimethylaminohexadecyl	523:544	arg1	methacrylate					546:557	Dimethylaminohexadecyl methacrylate	523:557	Dimethylaminohexadecyl methacrylate (DMAHDM)	523:566	METHODS Dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP) were incorporated into composite.
32800353	13	73	theme	enamel	1986:1991	arg1	hardness					1993:2000	enamel hardness	1986:2000	enamel hardness that was 2-fold greater than that of control composites	1986:2056	It substantially reduced enamel demineralization adjacent to restorations under biofilm acid attacks, yielding enamel hardness that was 2-fold greater than that of control composites.
32800353	4	74	theme	%	759:759	arg1	NACP					761:764	0% DMAHDM + 0% NACP	746:764	NACP	761:764	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	0	75	theme	enamel-restoration	128:145	arg1	margins					147:153	bovine enamel-restoration margins	121:153	bovine enamel-restoration margins	121:153	In vitro evaluation of composite containing DMAHDM and calcium phosphate nanoparticles on recurrent caries inhibition at bovine enamel-restoration margins.
32800353	1	76	theme	restoration	207:217	arg1	failure					219:225	restoration failure	207:225	restoration failure caused by biofilm acids	207:249	OBJECTIVE Recurrent caries is a primary reason for restoration failure caused by biofilm acids.
32800353	4	77	theme	3	793:793	arg1	%					794:794	%	794:794	%	794:794	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	8	78	theme	variance	1232:1239	arg1	analyses					1220:1227	one-way and two-way analyses	1200:1227	one-way and two-way analyses of variance	1200:1239	Data were analyzed via one-way and two-way analyses of variance and Tukey's multiple comparison tests.
32800353	10	79	theme	ion	1392:1394	arg1	release					1396:1402	Ca and P ion release	1383:1402	release	1396:1402	Ca and P ion release of 3% DMAHDM + 30% NACP was increased at cariogenic low pH. Biofilm lactic acid and polysaccharides were greatly decreased via DMAHDM, and CFU was reduced by 4 logs (P < 0.05).
32800353	13	80	theme	acid	1963:1966	arg1	attacks					1968:1974	biofilm acid attacks	1955:1974	biofilm acid attacks	1955:1974	It substantially reduced enamel demineralization adjacent to restorations under biofilm acid attacks, yielding enamel hardness that was 2-fold greater than that of control composites.
32800353	8	81	theme	multiple	1253:1260	arg1	tests					1273:1277	Tukey's multiple comparison tests	1245:1277	Tukey's multiple comparison tests	1245:1277	Data were analyzed via one-way and two-way analyses of variance and Tukey's multiple comparison tests.
32800353	3	82	theme	amorphous	589:597	arg1	NACP					618:621	NACP	618:621	NACP	618:621	METHODS Dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP) were incorporated into composite.
32800353	3	82	theme	amorphous	589:597	arg1	phosphate					607:615	amorphous calcium phosphate	589:615	amorphous calcium phosphate (NACP)	589:622	METHODS Dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP) were incorporated into composite.
32800353	7	83	theme	enamel	1147:1152	arg1	hardness					1154:1161	enamel hardness	1147:1161	enamel hardness	1147:1161	Demineralization of bovine enamel with restorations was induced via S. mutans, and enamel hardness was measured.
32800353	2	84	dep	develop	294:300	arg1	1					291:291	1	291:291	1	291:291	The objectives of this study were to: (1) develop a novel multifunctional composite with antibacterial function and calcium (Ca) and phosphate (P) ion release, and (2) investigate the effects on enamel demineralization and hardness at the margins under biofilms.
32800353	0	85	theme	phosphate	63:71	arg1	evaluation					9:18	In vitro evaluation	0:18	In vitro evaluation of composite containing DMAHDM and calcium phosphate	0:71	In vitro evaluation of composite containing DMAHDM and calcium phosphate nanoparticles on recurrent caries inhibition at bovine enamel-restoration margins.
32800353	1	86	theme	OBJECTIVE	156:164	arg1	reason					196:201	a primary reason	186:201	a primary reason for restoration failure caused by biofilm acids	186:249	OBJECTIVE Recurrent caries is a primary reason for restoration failure caused by biofilm acids.
32800353	1	86	theme	OBJECTIVE	156:164	arg1	caries					176:181	OBJECTIVE Recurrent caries	156:181	OBJECTIVE Recurrent caries	156:181	OBJECTIVE Recurrent caries is a primary reason for restoration failure caused by biofilm acids.
32800353	12	87	contain	had	1841:1843	arg1	CONCLUSIONS					1788:1798	CONCLUSIONS The novel 3% DMAHDM + 30% NACP composite	1788:1839	CONCLUSIONS The novel 3% DMAHDM + 30% NACP composite	1788:1839	CONCLUSIONS The novel 3% DMAHDM + 30% NACP composite had strong antibacterial effects.
32800353	12	87	contain	had	1841:1843	arg2	effects					1866:1872	strong antibacterial effects	1845:1872	strong antibacterial effects	1845:1872	CONCLUSIONS The novel 3% DMAHDM + 30% NACP composite had strong antibacterial effects.
32800353	2	88	from	effects	436:442	arg1	hardness					475:482	hardness	475:482	hardness	475:482	The objectives of this study were to: (1) develop a novel multifunctional composite with antibacterial function and calcium (Ca) and phosphate (P) ion release, and (2) investigate the effects on enamel demineralization and hardness at the margins under biofilms.
32800353	2	88	from	effects	436:442	arg1	demineralization					454:469	enamel demineralization	447:469	enamel demineralization	447:469	The objectives of this study were to: (1) develop a novel multifunctional composite with antibacterial function and calcium (Ca) and phosphate (P) ion release, and (2) investigate the effects on enamel demineralization and hardness at the margins under biofilms.
32800353	0	89	theme	caries	100:105	arg1	inhibition					107:116	recurrent caries inhibition	90:116	recurrent caries inhibition at bovine enamel-restoration margins	90:153	In vitro evaluation of composite containing DMAHDM and calcium phosphate nanoparticles on recurrent caries inhibition at bovine enamel-restoration margins.
32800353	2	90	dep	investigate	420:430	arg1	2					417:417	2	417:417	2	417:417	The objectives of this study were to: (1) develop a novel multifunctional composite with antibacterial function and calcium (Ca) and phosphate (P) ion release, and (2) investigate the effects on enamel demineralization and hardness at the margins under biofilms.
32800353	4	91	theme	0	805:805	arg1	%					806:806	%	806:806	%	806:806	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	12	92	theme	strong	1845:1850	arg1	effects					1866:1872	strong antibacterial effects	1845:1872	strong antibacterial effects	1845:1872	CONCLUSIONS The novel 3% DMAHDM + 30% NACP composite had strong antibacterial effects.
32800353	2	93	theme	novel	304:308	arg1	composite					326:334	a novel multifunctional composite	302:334	a novel multifunctional composite with antibacterial function and calcium (Ca) and phosphate (P) ion release	302:409	The objectives of this study were to: (1) develop a novel multifunctional composite with antibacterial function and calcium (Ca) and phosphate (P) ion release, and (2) investigate the effects on enamel demineralization and hardness at the margins under biofilms.
32800353	5	94	theme	Ca	908:909	arg1	release					921:927	Ca and P ion release	908:927	Ca and P ion release	908:927	Mechanical properties and Ca and P ion release were measured.
32800353	10	95	theme	3	1407:1407	arg1	%					1408:1408	%	1408:1408	%	1408:1408	Ca and P ion release of 3% DMAHDM + 30% NACP was increased at cariogenic low pH. Biofilm lactic acid and polysaccharides were greatly decreased via DMAHDM, and CFU was reduced by 4 logs (P < 0.05).
32800353	5	96	theme	P	915:915	arg1	release					921:927	Ca and P ion release	908:927	Ca and P ion release	908:927	Mechanical properties and Ca and P ion release were measured.
32800353	9	97	theme	mechanical	1349:1358	arg1	properties					1360:1369	the mechanical properties	1345:1369	the mechanical properties (P > 0.05)	1345:1380	RESULTS Adding DMAHDM and NACP into composite did not compromise the mechanical properties (P > 0.05).
32800353	9	97	theme	mechanical	1349:1358	arg1	>					1374:1374	P > 0.05	1372:1379	P > 0.05	1372:1379	RESULTS Adding DMAHDM and NACP into composite did not compromise the mechanical properties (P > 0.05).
32800353	12	98	theme	%	1824:1824	arg1	composite					1831:1839	30% NACP composite	1822:1839	30% NACP composite	1822:1839	CONCLUSIONS The novel 3% DMAHDM + 30% NACP composite had strong antibacterial effects.
32800353	2	99	theme	ion	399:401	arg1	release					403:409	calcium (Ca) and phosphate (P) ion release	368:409	calcium (Ca) and phosphate (P) ion release	368:409	The objectives of this study were to: (1) develop a novel multifunctional composite with antibacterial function and calcium (Ca) and phosphate (P) ion release, and (2) investigate the effects on enamel demineralization and hardness at the margins under biofilms.
32800353	10	100	theme	30	1419:1420	arg1	%					1421:1421	%	1421:1421	%	1421:1421	Ca and P ion release of 3% DMAHDM + 30% NACP was increased at cariogenic low pH. Biofilm lactic acid and polysaccharides were greatly decreased via DMAHDM, and CFU was reduced by 4 logs (P < 0.05).
32800353	0	101	theme	containing	33:42	arg1	DMAHDM					44:49	composite containing DMAHDM	23:49	composite containing DMAHDM	23:49	In vitro evaluation of composite containing DMAHDM and calcium phosphate nanoparticles on recurrent caries inhibition at bovine enamel-restoration margins.
32800353	7	102	theme	bovine	1084:1089	arg1	enamel					1091:1096	bovine enamel	1084:1096	bovine enamel	1084:1096	Demineralization of bovine enamel with restorations was induced via S. mutans, and enamel hardness was measured.
32800353	3	103	theme	phosphate	607:615	arg1	DMAHDM					560:565	DMAHDM	560:565	DMAHDM	560:565	METHODS Dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP) were incorporated into composite.
32800353	3	103	theme	phosphate	607:615	arg1	nanoparticles					572:584	nanoparticles	572:584	nanoparticles of amorphous calcium phosphate (NACP)	572:622	METHODS Dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP) were incorporated into composite.
32800353	3	103	theme	phosphate	607:615	arg1	methacrylate					546:557	Dimethylaminohexadecyl methacrylate	523:557	Dimethylaminohexadecyl methacrylate (DMAHDM)	523:566	METHODS Dimethylaminohexadecyl methacrylate (DMAHDM) and nanoparticles of amorphous calcium phosphate (NACP) were incorporated into composite.
32800353	12	104	theme	%	1811:1811	arg1	DMAHDM					1813:1818	The novel 3% DMAHDM	1800:1818	The novel 3% DMAHDM	1800:1818	CONCLUSIONS The novel 3% DMAHDM + 30% NACP composite had strong antibacterial effects.
32800353	2	105	theme	phosphate	385:393	arg1	release					403:409	calcium (Ca) and phosphate (P) ion release	368:409	calcium (Ca) and phosphate (P) ion release	368:409	The objectives of this study were to: (1) develop a novel multifunctional composite with antibacterial function and calcium (Ca) and phosphate (P) ion release, and (2) investigate the effects on enamel demineralization and hardness at the margins under biofilms.
32800353	2	106	with	composite	326:334	arg1	function					355:362	antibacterial function	341:362	antibacterial function	341:362	The objectives of this study were to: (1) develop a novel multifunctional composite with antibacterial function and calcium (Ca) and phosphate (P) ion release, and (2) investigate the effects on enamel demineralization and hardness at the margins under biofilms.
32800353	2	106	with	composite	326:334	arg1	release					403:409	calcium (Ca) and phosphate (P) ion release	368:409	calcium (Ca) and phosphate (P) ion release	368:409	The objectives of this study were to: (1) develop a novel multifunctional composite with antibacterial function and calcium (Ca) and phosphate (P) ion release, and (2) investigate the effects on enamel demineralization and hardness at the margins under biofilms.
32800353	4	107	theme	remineralizing	837:850	arg1	group					852:856	remineralizing group	837:856	remineralizing group (3% DMAHDM + 30% NACP)	837:879	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	4	108	theme	%	747:747	arg1	DMAHDM					749:754	0% DMAHDM + 0% NACP	746:764	DMAHDM	749:754	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	4	109	theme	Experimental	724:735	arg1	control					737:743	(2) Experimental control	720:743	(2) Experimental control (0% DMAHDM + 0% NACP)	720:765	Four groups were tested: (1) Commercial control (Heliomolar), (2) Experimental control (0% DMAHDM + 0% NACP), (3) antibacterial group (3% DMAHDM + 0% NACP), (D) antibacterial and remineralizing group (3% DMAHDM + 30% NACP).
32800353	2	110	from	margins	491:497	arg1	effects					436:442	the effects	432:442	the effects on enamel demineralization and hardness at the margins under biofilms	432:512	The objectives of this study were to: (1) develop a novel multifunctional composite with antibacterial function and calcium (Ca) and phosphate (P) ion release, and (2) investigate the effects on enamel demineralization and hardness at the margins under biofilms.
32800353	8	111	theme	one-way	1200:1206	arg1	analyses					1220:1227	one-way and two-way analyses	1200:1227	one-way and two-way analyses of variance	1200:1239	Data were analyzed via one-way and two-way analyses of variance and Tukey's multiple comparison tests.
32800353	14	112	theme	novel	2063:2067	arg1	composite					2085:2093	The novel multifunctional composite	2059:2093	The novel multifunctional composite	2059:2093	The novel multifunctional composite is promising to inhibit recurrent caries.
32800353	14	112	theme	novel	2063:2067	arg1	promising					2098:2106	promising	2098:2106	promising	2098:2106	The novel multifunctional composite is promising to inhibit recurrent caries.
32800353	10	113	theme	NACP	1423:1426	arg1	release					1396:1402	Ca and P ion release	1383:1402	release	1396:1402	Ca and P ion release of 3% DMAHDM + 30% NACP was increased at cariogenic low pH. Biofilm lactic acid and polysaccharides were greatly decreased via DMAHDM, and CFU was reduced by 4 logs (P < 0.05).
32800353	11	114	theme	enamel	1602:1607	arg1	hardness					1609:1616	enamel hardness	1602:1616	enamel hardness at the margins	1602:1631	Under biofilm acids, enamel hardness at the margins was decreased to about 0.5 GPa for control; it was about 1 GPa for antibacterial group, and 1.3 GPa for antibacterial and remineralizing group (P < 0.05).
32800353	8	115	theme	two-way	1212:1218	arg1	analyses					1220:1227	one-way and two-way analyses	1200:1227	one-way and two-way analyses of variance	1200:1239	Data were analyzed via one-way and two-way analyses of variance and Tukey's multiple comparison tests.
33069404	9	0	theme	negative	1508:1515	arg1	relationship					1540:1551	this negative, and generally linear, relationship	1503:1551	this negative, and generally linear, relationship between unsaturated C18 FA levels and milk OBCFA	1503:1600	Overall, regression equations supported this negative, and generally linear, relationship between unsaturated C18 FA levels and milk OBCFA.
33069404	12	1	theme	increasing	2092:2101	arg1	levels					2103:2108	increasing levels	2092:2108	increasing levels of acid detergent fiber (positive linear coefficients) and starch (negative linear coefficients)	2092:2205	In this regard, regression equations indicated that iso FA would show opposite responses to increasing levels of acid detergent fiber (positive linear coefficients) and starch (negative linear coefficients).
33069404	12	2	theme	linear	2186:2191	arg1	coefficients					2193:2204	negative linear coefficients	2177:2204	negative linear coefficients	2177:2204	In this regard, regression equations indicated that iso FA would show opposite responses to increasing levels of acid detergent fiber (positive linear coefficients) and starch (negative linear coefficients).
33069404	12	2	theme	linear	2186:2191	arg1	starch					2169:2174	starch	2169:2174	starch (negative linear coefficients)	2169:2205	In this regard, regression equations indicated that iso FA would show opposite responses to increasing levels of acid detergent fiber (positive linear coefficients) and starch (negative linear coefficients).
33069404	11	3	theme	dietary	1722:1728	arg1	determinant					1753:1763	a determinant	1751:1763	a determinant of milk OBCFA profile in dairy ewes	1751:1799	The PCA also suggested that dietary crude protein is not a determinant of milk OBCFA profile in dairy ewes, but significant relationships were observed between some OBCFA and dietary fiber or starch, consistent with a potential role of these FA as biomarkers of rumen cellulolytic and amylolytic bacteria.
33069404	11	3	theme	dietary	1722:1728	arg1	protein					1736:1742	dietary crude protein	1722:1742	dietary crude protein	1722:1742	The PCA also suggested that dietary crude protein is not a determinant of milk OBCFA profile in dairy ewes, but significant relationships were observed between some OBCFA and dietary fiber or starch, consistent with a potential role of these FA as biomarkers of rumen cellulolytic and amylolytic bacteria.
33069404	11	4	theme	cellulolytic	1962:1973	arg1	bacteria					1990:1997	rumen cellulolytic and amylolytic bacteria	1956:1997	rumen cellulolytic and amylolytic bacteria	1956:1997	The PCA also suggested that dietary crude protein is not a determinant of milk OBCFA profile in dairy ewes, but significant relationships were observed between some OBCFA and dietary fiber or starch, consistent with a potential role of these FA as biomarkers of rumen cellulolytic and amylolytic bacteria.
33069404	5	5	theme	lipid	803:807	arg1	supplements					809:819	lipid supplements	803:819	lipid supplements	803:819	A total of 47 lots received lipid supplements, whereas their respective controls (27 lots) were fed the same basal diets without lipid supplementation.
33069404	13	6	theme	feeding	2366:2372	arg1	conditions					2374:2383	different feeding conditions	2356:2383	different feeding conditions	2356:2383	Lipid supplementation would not largely affect these associations, supporting the potential of OBCFA as noninvasive markers of rumen function under different feeding conditions (i.e., with or without lipid supplementation).
33069404	10	7	theme	n-3	1636:1638	arg1	FA					1656:1657	C20-22 n-3 polyunsaturated FA	1629:1657	C20-22 n-3 polyunsaturated FA	1629:1657	However, the influence of C20-22 n-3 polyunsaturated FA and saturated FA was more limited.
33069404	14	8	theme	nutritional	2473:2483	arg1	benefits					2485:2492	nutritional benefits	2473:2492	nutritional benefits for humans	2473:2503	Because consumption of these FA may have nutritional benefits for humans, the use of high-fiber/low-starch rations might be recommended to maintain the highest possible content of milk OBCFA in dairy sheep.
33069404	9	9	theme	unsaturated	1561:1571	arg1	levels					1580:1585	unsaturated C18 FA levels	1561:1585	unsaturated C18 FA levels	1561:1585	Overall, regression equations supported this negative, and generally linear, relationship between unsaturated C18 FA levels and milk OBCFA.
33069404	11	10	theme	dairy	1790:1794	arg1	ewes					1796:1799	dairy ewes	1790:1799	dairy ewes	1790:1799	The PCA also suggested that dietary crude protein is not a determinant of milk OBCFA profile in dairy ewes, but significant relationships were observed between some OBCFA and dietary fiber or starch, consistent with a potential role of these FA as biomarkers of rumen cellulolytic and amylolytic bacteria.
33069404	0	11	theme	dairy	103:107	arg1	ewes					109:112	dairy ewes	103:112	dairy ewes	103:112	Effect of dietary lipids and other nutrients on milk odd- and branched-chain fatty acid composition in dairy ewes.
33069404	11	12	theme	dietary	1869:1875	arg1	fiber					1877:1881	dietary fiber	1869:1881	dietary fiber	1869:1881	The PCA also suggested that dietary crude protein is not a determinant of milk OBCFA profile in dairy ewes, but significant relationships were observed between some OBCFA and dietary fiber or starch, consistent with a potential role of these FA as biomarkers of rumen cellulolytic and amylolytic bacteria.
33069404	14	13	contain	have	2468:2471	arg2	benefits					2485:2492	nutritional benefits	2473:2492	nutritional benefits for humans	2473:2503	Because consumption of these FA may have nutritional benefits for humans, the use of high-fiber/low-starch rations might be recommended to maintain the highest possible content of milk OBCFA in dairy sheep.
33069404	14	13	contain	have	2468:2471	arg1	consumption					2440:2450	consumption	2440:2450	consumption of these FA	2440:2462	Because consumption of these FA may have nutritional benefits for humans, the use of high-fiber/low-starch rations might be recommended to maintain the highest possible content of milk OBCFA in dairy sheep.
33069404	9	14	theme	FA	1577:1578	arg1	levels					1580:1585	unsaturated C18 FA levels	1561:1585	unsaturated C18 FA levels	1561:1585	Overall, regression equations supported this negative, and generally linear, relationship between unsaturated C18 FA levels and milk OBCFA.
33069404	13	15	theme	rumen	2335:2339	arg1	function					2341:2348	rumen function	2335:2348	rumen function	2335:2348	Lipid supplementation would not largely affect these associations, supporting the potential of OBCFA as noninvasive markers of rumen function under different feeding conditions (i.e., with or without lipid supplementation).
33069404	0	16	theme	lipids	18:23	arg1	Effect					0:5	Effect	0:5	Effect of dietary lipids and other nutrients on milk odd- and branched-chain fatty acid composition in dairy ewes.	0:113	Effect of dietary lipids and other nutrients on milk odd- and branched-chain fatty acid composition in dairy ewes.
33069404	5	17	theme	lots	789:792	arg1	total					777:781	A total	775:781	A total of 47 lots	775:792	A total of 47 lots received lipid supplements, whereas their respective controls (27 lots) were fed the same basal diets without lipid supplementation.
33069404	12	18	theme	starch	2169:2174	arg1	levels					2103:2108	increasing levels	2092:2108	increasing levels of acid detergent fiber (positive linear coefficients) and starch (negative linear coefficients)	2092:2205	In this regard, regression equations indicated that iso FA would show opposite responses to increasing levels of acid detergent fiber (positive linear coefficients) and starch (negative linear coefficients).
33069404	14	19	theme	dairy	2626:2630	arg1	sheep					2632:2636	dairy sheep	2626:2636	dairy sheep	2626:2636	Because consumption of these FA may have nutritional benefits for humans, the use of high-fiber/low-starch rations might be recommended to maintain the highest possible content of milk OBCFA in dairy sheep.
33069404	13	20	dep	supporting	2275:2284	arg1	i.e.					2386:2389	i.e.	2386:2389	i.e.	2386:2389	Lipid supplementation would not largely affect these associations, supporting the potential of OBCFA as noninvasive markers of rumen function under different feeding conditions (i.e., with or without lipid supplementation).
33069404	10	21	theme	FA	1656:1657	arg1	influence					1616:1624	the influence	1612:1624	the influence of C20-22 n-3 polyunsaturated FA and saturated FA	1612:1674	However, the influence of C20-22 n-3 polyunsaturated FA and saturated FA was more limited.
33069404	10	21	theme	FA	1656:1657	arg1	limited					1685:1691	limited	1685:1691	limited	1685:1691	However, the influence of C20-22 n-3 polyunsaturated FA and saturated FA was more limited.
33069404	0	22	from	composition	88:98	arg1	ewes					109:112	dairy ewes	103:112	dairy ewes	103:112	Effect of dietary lipids and other nutrients on milk odd- and branched-chain fatty acid composition in dairy ewes.
33069404	14	23	theme	OBCFA	2617:2621	arg1	content					2601:2607	the highest possible content	2580:2607	the highest possible content of milk OBCFA in dairy sheep	2580:2636	Because consumption of these FA may have nutritional benefits for humans, the use of high-fiber/low-starch rations might be recommended to maintain the highest possible content of milk OBCFA in dairy sheep.
33069404	11	24	from	profile	1779:1785	arg1	ewes					1796:1799	dairy ewes	1790:1799	dairy ewes	1790:1799	The PCA also suggested that dietary crude protein is not a determinant of milk OBCFA profile in dairy ewes, but significant relationships were observed between some OBCFA and dietary fiber or starch, consistent with a potential role of these FA as biomarkers of rumen cellulolytic and amylolytic bacteria.
33069404	8	25	theme	C18	1290:1292	arg1	FA					1294:1295	dietary unsaturated C18 FA	1270:1295	dietary unsaturated C18 FA	1270:1295	According to the loading plot, dietary unsaturated C18 FA loaded opposite to major milk OBCFA (e.g., 15:0, 15:0 anteiso, and 17:0) and were strongly correlated with principal component 1, which described 46% of variability.
33069404	11	26	dep	OBCFA	1859:1863	arg1	some					1854:1857	some	1854:1857	some	1854:1857	The PCA also suggested that dietary crude protein is not a determinant of milk OBCFA profile in dairy ewes, but significant relationships were observed between some OBCFA and dietary fiber or starch, consistent with a potential role of these FA as biomarkers of rumen cellulolytic and amylolytic bacteria.
33069404	2	27	theme	nutrients	450:458	arg1	effects					422:428	the effects	418:428	the effects of lipids and other nutrients on milk OBCFA	418:472	Targeted research has examined relationships between these fatty acids (FA) and dietary components, but interactions between the effects of lipids and other nutrients on milk OBCFA are not well characterized yet.
33069404	12	28	theme	linear	2144:2149	arg1	coefficients					2151:2162	positive linear coefficients	2135:2162	positive linear coefficients	2135:2162	In this regard, regression equations indicated that iso FA would show opposite responses to increasing levels of acid detergent fiber (positive linear coefficients) and starch (negative linear coefficients).
33069404	12	28	theme	linear	2144:2149	arg1	fiber					2128:2132	acid detergent fiber	2113:2132	acid detergent fiber (positive linear coefficients)	2113:2163	In this regard, regression equations indicated that iso FA would show opposite responses to increasing levels of acid detergent fiber (positive linear coefficients) and starch (negative linear coefficients).
33069404	11	29	theme	OBCFA	1773:1777	arg1	profile					1779:1785	milk OBCFA profile	1768:1785	milk OBCFA profile in dairy ewes	1768:1799	The PCA also suggested that dietary crude protein is not a determinant of milk OBCFA profile in dairy ewes, but significant relationships were observed between some OBCFA and dietary fiber or starch, consistent with a potential role of these FA as biomarkers of rumen cellulolytic and amylolytic bacteria.
33069404	4	30	theme	diet	643:646	arg1	composition					648:658	diet composition	643:658	diet composition	643:658	Thus, the present meta-analysis examined relationships between diet composition and milk OBCFA using a database compiled with lot observations from 14 trials in dairy ewes fed lipid supplements.
33069404	0	31	theme	milk	48:51	arg1	composition					88:98	milk odd- and branched-chain fatty acid composition	48:98	milk odd- and branched-chain fatty acid composition in dairy ewes	48:112	Effect of dietary lipids and other nutrients on milk odd- and branched-chain fatty acid composition in dairy ewes.
33069404	1	32	theme	function	283:290	arg1	biomarkers					263:272	biomarkers	263:272	biomarkers of rumen function	263:290	Milk odd- and branched-chain fatty acids (OBCFA) are largely derived from bacteria leaving the rumen, which has encouraged research on their use as biomarkers of rumen function.
33069404	7	33	theme	milk	1095:1098	arg1	OBCFA					1100:1104	milk OBCFA	1095:1104	milk OBCFA	1095:1104	Then, responses of milk OBCFA to variations in specific dietary components (selected on the basis of the PCA) were examined in more detail by regression analysis.
33069404	6	34	theme	milk	949:952	arg1	OBCFA					954:958	milk OBCFA	949:958	milk OBCFA	949:958	Relationships between milk OBCFA and dietary components were first assessed through a principal component analysis (PCA) and a correlation analysis.
33069404	11	35	from	determinant	1753:1763	arg1	ewes					1796:1799	dairy ewes	1790:1799	dairy ewes	1790:1799	The PCA also suggested that dietary crude protein is not a determinant of milk OBCFA profile in dairy ewes, but significant relationships were observed between some OBCFA and dietary fiber or starch, consistent with a potential role of these FA as biomarkers of rumen cellulolytic and amylolytic bacteria.
33069404	4	36	from	trials	731:736	arg1	ewes					747:750	dairy ewes	741:750	dairy ewes fed lipid supplements	741:772	Thus, the present meta-analysis examined relationships between diet composition and milk OBCFA using a database compiled with lot observations from 14 trials in dairy ewes fed lipid supplements.
33069404	4	36	from	trials	731:736	arg1	observations					710:721	lot observations	706:721	lot observations from 14 trials in dairy ewes fed lipid supplements	706:772	Thus, the present meta-analysis examined relationships between diet composition and milk OBCFA using a database compiled with lot observations from 14 trials in dairy ewes fed lipid supplements.
33069404	1	37	theme	fatty	144:148	arg1	acids					150:154	Milk odd- and branched-chain fatty acids	115:154	acids	150:154	Milk odd- and branched-chain fatty acids (OBCFA) are largely derived from bacteria leaving the rumen, which has encouraged research on their use as biomarkers of rumen function.
33069404	0	38	from	Effect	0:5	arg1	composition					88:98	milk odd- and branched-chain fatty acid composition	48:98	milk odd- and branched-chain fatty acid composition in dairy ewes	48:112	Effect of dietary lipids and other nutrients on milk odd- and branched-chain fatty acid composition in dairy ewes.
33069404	0	39	theme	fatty	77:81	arg1	composition					88:98	milk odd- and branched-chain fatty acid composition	48:98	milk odd- and branched-chain fatty acid composition in dairy ewes	48:112	Effect of dietary lipids and other nutrients on milk odd- and branched-chain fatty acid composition in dairy ewes.
33069404	2	40	theme	milk	463:466	arg1	OBCFA					468:472	milk OBCFA	463:472	milk OBCFA	463:472	Targeted research has examined relationships between these fatty acids (FA) and dietary components, but interactions between the effects of lipids and other nutrients on milk OBCFA are not well characterized yet.
33069404	12	41	theme	opposite	2070:2077	arg1	responses					2079:2087	opposite responses	2070:2087	opposite responses to increasing levels of acid detergent fiber (positive linear coefficients) and starch (negative linear coefficients)	2070:2205	In this regard, regression equations indicated that iso FA would show opposite responses to increasing levels of acid detergent fiber (positive linear coefficients) and starch (negative linear coefficients).
33069404	5	42	theme	lipid	904:908	arg1	supplementation					910:924	lipid supplementation	904:924	lipid supplementation	904:924	A total of 47 lots received lipid supplements, whereas their respective controls (27 lots) were fed the same basal diets without lipid supplementation.
33069404	6	43	theme	principal	1013:1021	arg1	PCA					1043:1045	PCA	1043:1045	PCA	1043:1045	Relationships between milk OBCFA and dietary components were first assessed through a principal component analysis (PCA) and a correlation analysis.
33069404	6	43	theme	principal	1013:1021	arg1	analysis					1033:1040	a principal component analysis	1011:1040	a principal component analysis (PCA)	1011:1046	Relationships between milk OBCFA and dietary components were first assessed through a principal component analysis (PCA) and a correlation analysis.
33069404	2	44	from	effects	422:428	arg1	OBCFA					468:472	milk OBCFA	463:472	milk OBCFA	463:472	Targeted research has examined relationships between these fatty acids (FA) and dietary components, but interactions between the effects of lipids and other nutrients on milk OBCFA are not well characterized yet.
33069404	8	45	theme	dietary	1270:1276	arg1	FA					1294:1295	dietary unsaturated C18 FA	1270:1295	dietary unsaturated C18 FA	1270:1295	According to the loading plot, dietary unsaturated C18 FA loaded opposite to major milk OBCFA (e.g., 15:0, 15:0 anteiso, and 17:0) and were strongly correlated with principal component 1, which described 46% of variability.
33069404	2	46	theme	fatty	352:356	arg1	acids					358:362	these fatty acids	346:362	these fatty acids (FA)	346:367	Targeted research has examined relationships between these fatty acids (FA) and dietary components, but interactions between the effects of lipids and other nutrients on milk OBCFA are not well characterized yet.
33069404	2	46	theme	fatty	352:356	arg1	FA					365:366	FA	365:366	FA	365:366	Targeted research has examined relationships between these fatty acids (FA) and dietary components, but interactions between the effects of lipids and other nutrients on milk OBCFA are not well characterized yet.
33069404	11	47	theme	amylolytic	1979:1988	arg1	bacteria					1990:1997	rumen cellulolytic and amylolytic bacteria	1956:1997	rumen cellulolytic and amylolytic bacteria	1956:1997	The PCA also suggested that dietary crude protein is not a determinant of milk OBCFA profile in dairy ewes, but significant relationships were observed between some OBCFA and dietary fiber or starch, consistent with a potential role of these FA as biomarkers of rumen cellulolytic and amylolytic bacteria.
33069404	7	48	dep	components	1140:1149	arg1	selected					1152:1159	selected	1152:1159	selected on the basis of the PCA	1152:1183	Then, responses of milk OBCFA to variations in specific dietary components (selected on the basis of the PCA) were examined in more detail by regression analysis.
33069404	4	49	theme	lot	706:708	arg1	observations					710:721	lot observations	706:721	lot observations from 14 trials in dairy ewes fed lipid supplements	706:772	Thus, the present meta-analysis examined relationships between diet composition and milk OBCFA using a database compiled with lot observations from 14 trials in dairy ewes fed lipid supplements.
33069404	2	50	theme	dietary	373:379	arg1	components					381:390	dietary components	373:390	dietary components	373:390	Targeted research has examined relationships between these fatty acids (FA) and dietary components, but interactions between the effects of lipids and other nutrients on milk OBCFA are not well characterized yet.
33069404	0	51	theme	dietary	10:16	arg1	lipids					18:23	dietary lipids	10:23	dietary lipids	10:23	Effect of dietary lipids and other nutrients on milk odd- and branched-chain fatty acid composition in dairy ewes.
33069404	5	52	theme	same	879:882	arg1	diets					890:894	the same basal diets	875:894	the same basal diets	875:894	A total of 47 lots received lipid supplements, whereas their respective controls (27 lots) were fed the same basal diets without lipid supplementation.
33069404	13	53	theme	Lipid	2208:2212	arg1	supplementation					2214:2228	Lipid supplementation	2208:2228	Lipid supplementation	2208:2228	Lipid supplementation would not largely affect these associations, supporting the potential of OBCFA as noninvasive markers of rumen function under different feeding conditions (i.e., with or without lipid supplementation).
33069404	7	54	theme	more	1203:1206	arg1	detail					1208:1213	more detail	1203:1213	more detail	1203:1213	Then, responses of milk OBCFA to variations in specific dietary components (selected on the basis of the PCA) were examined in more detail by regression analysis.
33069404	12	55	theme	regression	2016:2025	arg1	equations					2027:2035	regression equations	2016:2035	regression equations	2016:2035	In this regard, regression equations indicated that iso FA would show opposite responses to increasing levels of acid detergent fiber (positive linear coefficients) and starch (negative linear coefficients).
33069404	0	56	theme	nutrients	35:43	arg1	Effect					0:5	Effect	0:5	Effect of dietary lipids and other nutrients on milk odd- and branched-chain fatty acid composition in dairy ewes.	0:113	Effect of dietary lipids and other nutrients on milk odd- and branched-chain fatty acid composition in dairy ewes.
33069404	6	57	theme	correlation	1054:1064	arg1	analysis					1066:1073	a correlation analysis	1052:1073	a correlation analysis	1052:1073	Relationships between milk OBCFA and dietary components were first assessed through a principal component analysis (PCA) and a correlation analysis.
33069404	11	58	theme	significant	1806:1816	arg1	relationships					1818:1830	significant relationships	1806:1830	significant relationships	1806:1830	The PCA also suggested that dietary crude protein is not a determinant of milk OBCFA profile in dairy ewes, but significant relationships were observed between some OBCFA and dietary fiber or starch, consistent with a potential role of these FA as biomarkers of rumen cellulolytic and amylolytic bacteria.
33069404	8	59	theme	milk	1322:1325	arg1	OBCFA					1327:1331	major milk OBCFA	1316:1331	major milk OBCFA (e.g., 15:0, 15:0 anteiso, and 17:0)	1316:1368	According to the loading plot, dietary unsaturated C18 FA loaded opposite to major milk OBCFA (e.g., 15:0, 15:0 anteiso, and 17:0) and were strongly correlated with principal component 1, which described 46% of variability.
33069404	8	60	theme	variability	1450:1460	arg1	variability					1450:1460	variability	1450:1460	variability	1450:1460	According to the loading plot, dietary unsaturated C18 FA loaded opposite to major milk OBCFA (e.g., 15:0, 15:0 anteiso, and 17:0) and were strongly correlated with principal component 1, which described 46% of variability.
33069404	8	60	theme	variability	1450:1460	arg1	%					1445:1445	46%	1443:1445	46% of variability	1443:1460	According to the loading plot, dietary unsaturated C18 FA loaded opposite to major milk OBCFA (e.g., 15:0, 15:0 anteiso, and 17:0) and were strongly correlated with principal component 1, which described 46% of variability.
33069404	1	61	attach	derived	176:182	arg2	acids					150:154	Milk odd- and branched-chain fatty acids	115:154	acids	150:154	Milk odd- and branched-chain fatty acids (OBCFA) are largely derived from bacteria leaving the rumen, which has encouraged research on their use as biomarkers of rumen function.
33069404	1	61	attach	derived	176:182	arg1	bacteria					189:196	bacteria	189:196	bacteria leaving the rumen, which has encouraged research on their use as biomarkers of rumen function	189:290	Milk odd- and branched-chain fatty acids (OBCFA) are largely derived from bacteria leaving the rumen, which has encouraged research on their use as biomarkers of rumen function.
33069404	1	61	attach	derived	176:182	arg2	odd-					120:123	odd-	120:123	odd-	120:123	Milk odd- and branched-chain fatty acids (OBCFA) are largely derived from bacteria leaving the rumen, which has encouraged research on their use as biomarkers of rumen function.
33069404	1	61	attach	derived	176:182	arg2	OBCFA					157:161	OBCFA	157:161	OBCFA	157:161	Milk odd- and branched-chain fatty acids (OBCFA) are largely derived from bacteria leaving the rumen, which has encouraged research on their use as biomarkers of rumen function.
33069404	14	62	from	content	2601:2607	arg1	sheep					2632:2636	dairy sheep	2626:2636	dairy sheep	2626:2636	Because consumption of these FA may have nutritional benefits for humans, the use of high-fiber/low-starch rations might be recommended to maintain the highest possible content of milk OBCFA in dairy sheep.
33069404	11	63	theme	crude	1730:1734	arg1	determinant					1753:1763	a determinant	1751:1763	a determinant of milk OBCFA profile in dairy ewes	1751:1799	The PCA also suggested that dietary crude protein is not a determinant of milk OBCFA profile in dairy ewes, but significant relationships were observed between some OBCFA and dietary fiber or starch, consistent with a potential role of these FA as biomarkers of rumen cellulolytic and amylolytic bacteria.
33069404	11	63	theme	crude	1730:1734	arg1	protein					1736:1742	dietary crude protein	1722:1742	dietary crude protein	1722:1742	The PCA also suggested that dietary crude protein is not a determinant of milk OBCFA profile in dairy ewes, but significant relationships were observed between some OBCFA and dietary fiber or starch, consistent with a potential role of these FA as biomarkers of rumen cellulolytic and amylolytic bacteria.
33069404	13	64	theme	different	2356:2364	arg1	conditions					2374:2383	different feeding conditions	2356:2383	different feeding conditions	2356:2383	Lipid supplementation would not largely affect these associations, supporting the potential of OBCFA as noninvasive markers of rumen function under different feeding conditions (i.e., with or without lipid supplementation).
33069404	12	65	theme	negative	2177:2184	arg1	coefficients					2193:2204	negative linear coefficients	2177:2204	negative linear coefficients	2177:2204	In this regard, regression equations indicated that iso FA would show opposite responses to increasing levels of acid detergent fiber (positive linear coefficients) and starch (negative linear coefficients).
33069404	12	65	theme	negative	2177:2184	arg1	starch					2169:2174	starch	2169:2174	starch (negative linear coefficients)	2169:2205	In this regard, regression equations indicated that iso FA would show opposite responses to increasing levels of acid detergent fiber (positive linear coefficients) and starch (negative linear coefficients).
33069404	11	66	theme	FA	1936:1937	arg1	role					1922:1925	a potential role	1910:1925	a potential role of these FA as biomarkers of rumen cellulolytic and amylolytic bacteria	1910:1997	The PCA also suggested that dietary crude protein is not a determinant of milk OBCFA profile in dairy ewes, but significant relationships were observed between some OBCFA and dietary fiber or starch, consistent with a potential role of these FA as biomarkers of rumen cellulolytic and amylolytic bacteria.
33069404	5	67	theme	respective	836:845	arg1	controls					847:854	their respective controls	830:854	their respective controls (27 lots)	830:864	A total of 47 lots received lipid supplements, whereas their respective controls (27 lots) were fed the same basal diets without lipid supplementation.
33069404	5	67	theme	respective	836:845	arg1	lots					860:863	27 lots	857:863	27 lots	857:863	A total of 47 lots received lipid supplements, whereas their respective controls (27 lots) were fed the same basal diets without lipid supplementation.
33069404	3	68	theme	milk	539:542	arg1	OBCFA					544:548	milk OBCFA	539:548	milk OBCFA	539:548	Furthermore, factors controlling milk OBCFA in sheep are largely unknown.
33069404	13	69	theme	function	2341:2348	arg1	markers					2324:2330	noninvasive markers	2312:2330	noninvasive markers of rumen function	2312:2348	Lipid supplementation would not largely affect these associations, supporting the potential of OBCFA as noninvasive markers of rumen function under different feeding conditions (i.e., with or without lipid supplementation).
33069404	13	70	theme	lipid	2408:2412	arg1	supplementation					2414:2428	lipid supplementation	2408:2428	lipid supplementation	2408:2428	Lipid supplementation would not largely affect these associations, supporting the potential of OBCFA as noninvasive markers of rumen function under different feeding conditions (i.e., with or without lipid supplementation).
33069404	4	71	theme	present	590:596	arg1	meta-analysis					598:610	the present meta-analysis	586:610	the present meta-analysis	586:610	Thus, the present meta-analysis examined relationships between diet composition and milk OBCFA using a database compiled with lot observations from 14 trials in dairy ewes fed lipid supplements.
33069404	1	72	from	research	238:245	arg1	use					256:258	their use	250:258	their use as biomarkers of rumen function	250:290	Milk odd- and branched-chain fatty acids (OBCFA) are largely derived from bacteria leaving the rumen, which has encouraged research on their use as biomarkers of rumen function.
33069404	10	73	theme	polyunsaturated	1640:1654	arg1	FA					1656:1657	C20-22 n-3 polyunsaturated FA	1629:1657	C20-22 n-3 polyunsaturated FA	1629:1657	However, the influence of C20-22 n-3 polyunsaturated FA and saturated FA was more limited.
33069404	12	74	theme	iso	2052:2054	arg1	FA					2056:2057	iso FA	2052:2057	iso FA	2052:2057	In this regard, regression equations indicated that iso FA would show opposite responses to increasing levels of acid detergent fiber (positive linear coefficients) and starch (negative linear coefficients).
33069404	13	75	theme	noninvasive	2312:2322	arg1	markers					2324:2330	noninvasive markers	2312:2330	noninvasive markers of rumen function	2312:2348	Lipid supplementation would not largely affect these associations, supporting the potential of OBCFA as noninvasive markers of rumen function under different feeding conditions (i.e., with or without lipid supplementation).
33069404	10	76	theme	C20-22	1629:1634	arg1	FA					1656:1657	C20-22 n-3 polyunsaturated FA	1629:1657	C20-22 n-3 polyunsaturated FA	1629:1657	However, the influence of C20-22 n-3 polyunsaturated FA and saturated FA was more limited.
33069404	9	77	theme	C18	1573:1575	arg1	levels					1580:1585	unsaturated C18 FA levels	1561:1585	unsaturated C18 FA levels	1561:1585	Overall, regression equations supported this negative, and generally linear, relationship between unsaturated C18 FA levels and milk OBCFA.
33069404	7	78	from	variations	1109:1118	arg1	components					1140:1149	specific dietary components	1123:1149	specific dietary components (selected on the basis of the PCA)	1123:1184	Then, responses of milk OBCFA to variations in specific dietary components (selected on the basis of the PCA) were examined in more detail by regression analysis.
33069404	11	79	from	ewes	1796:1799	arg1	determinant					1753:1763	a determinant	1751:1763	a determinant of milk OBCFA profile in dairy ewes	1751:1799	The PCA also suggested that dietary crude protein is not a determinant of milk OBCFA profile in dairy ewes, but significant relationships were observed between some OBCFA and dietary fiber or starch, consistent with a potential role of these FA as biomarkers of rumen cellulolytic and amylolytic bacteria.
33069404	11	79	from	ewes	1796:1799	arg1	protein					1736:1742	dietary crude protein	1722:1742	dietary crude protein	1722:1742	The PCA also suggested that dietary crude protein is not a determinant of milk OBCFA profile in dairy ewes, but significant relationships were observed between some OBCFA and dietary fiber or starch, consistent with a potential role of these FA as biomarkers of rumen cellulolytic and amylolytic bacteria.
33069404	14	80	theme	high-fiber/low-starch	2517:2537	arg1	rations					2539:2545	high-fiber/low-starch rations	2517:2545	high-fiber/low-starch rations	2517:2545	Because consumption of these FA may have nutritional benefits for humans, the use of high-fiber/low-starch rations might be recommended to maintain the highest possible content of milk OBCFA in dairy sheep.
33069404	10	81	theme	FA	1673:1674	arg1	influence					1616:1624	the influence	1612:1624	the influence of C20-22 n-3 polyunsaturated FA and saturated FA	1612:1674	However, the influence of C20-22 n-3 polyunsaturated FA and saturated FA was more limited.
33069404	10	81	theme	FA	1673:1674	arg1	limited					1685:1691	limited	1685:1691	limited	1685:1691	However, the influence of C20-22 n-3 polyunsaturated FA and saturated FA was more limited.
33069404	14	82	theme	FA	2461:2462	arg1	consumption					2440:2450	consumption	2440:2450	consumption of these FA	2440:2462	Because consumption of these FA may have nutritional benefits for humans, the use of high-fiber/low-starch rations might be recommended to maintain the highest possible content of milk OBCFA in dairy sheep.
33069404	7	83	theme	specific	1123:1130	arg1	components					1140:1149	specific dietary components	1123:1149	specific dietary components (selected on the basis of the PCA)	1123:1184	Then, responses of milk OBCFA to variations in specific dietary components (selected on the basis of the PCA) were examined in more detail by regression analysis.
33069404	12	84	theme	detergent	2118:2126	arg1	coefficients					2151:2162	positive linear coefficients	2135:2162	positive linear coefficients	2135:2162	In this regard, regression equations indicated that iso FA would show opposite responses to increasing levels of acid detergent fiber (positive linear coefficients) and starch (negative linear coefficients).
33069404	12	84	theme	detergent	2118:2126	arg1	fiber					2128:2132	acid detergent fiber	2113:2132	acid detergent fiber (positive linear coefficients)	2113:2163	In this regard, regression equations indicated that iso FA would show opposite responses to increasing levels of acid detergent fiber (positive linear coefficients) and starch (negative linear coefficients).
33069404	9	85	theme	milk	1591:1594	arg1	OBCFA					1596:1600	milk OBCFA	1591:1600	milk OBCFA	1591:1600	Overall, regression equations supported this negative, and generally linear, relationship between unsaturated C18 FA levels and milk OBCFA.
33069404	12	86	theme	positive	2135:2142	arg1	coefficients					2151:2162	positive linear coefficients	2135:2162	positive linear coefficients	2135:2162	In this regard, regression equations indicated that iso FA would show opposite responses to increasing levels of acid detergent fiber (positive linear coefficients) and starch (negative linear coefficients).
33069404	12	86	theme	positive	2135:2142	arg1	fiber					2128:2132	acid detergent fiber	2113:2132	acid detergent fiber (positive linear coefficients)	2113:2163	In this regard, regression equations indicated that iso FA would show opposite responses to increasing levels of acid detergent fiber (positive linear coefficients) and starch (negative linear coefficients).
33069404	4	87	theme	milk	664:667	arg1	OBCFA					669:673	milk OBCFA	664:673	milk OBCFA	664:673	Thus, the present meta-analysis examined relationships between diet composition and milk OBCFA using a database compiled with lot observations from 14 trials in dairy ewes fed lipid supplements.
33069404	13	88	theme	OBCFA	2303:2307	arg1	potential					2290:2298	the potential	2286:2298	the potential of OBCFA as noninvasive markers of rumen function	2286:2348	Lipid supplementation would not largely affect these associations, supporting the potential of OBCFA as noninvasive markers of rumen function under different feeding conditions (i.e., with or without lipid supplementation).
33069404	4	89	theme	dairy	741:745	arg1	ewes					747:750	dairy ewes	741:750	dairy ewes fed lipid supplements	741:772	Thus, the present meta-analysis examined relationships between diet composition and milk OBCFA using a database compiled with lot observations from 14 trials in dairy ewes fed lipid supplements.
33069404	6	90	theme	dietary	964:970	arg1	components					972:981	dietary components	964:981	dietary components	964:981	Relationships between milk OBCFA and dietary components were first assessed through a principal component analysis (PCA) and a correlation analysis.
33069404	11	91	theme	potential	1912:1920	arg1	role					1922:1925	a potential role	1910:1925	a potential role of these FA as biomarkers of rumen cellulolytic and amylolytic bacteria	1910:1997	The PCA also suggested that dietary crude protein is not a determinant of milk OBCFA profile in dairy ewes, but significant relationships were observed between some OBCFA and dietary fiber or starch, consistent with a potential role of these FA as biomarkers of rumen cellulolytic and amylolytic bacteria.
33069404	14	92	theme	milk	2612:2615	arg1	OBCFA					2617:2621	milk OBCFA	2612:2621	milk OBCFA	2612:2621	Because consumption of these FA may have nutritional benefits for humans, the use of high-fiber/low-starch rations might be recommended to maintain the highest possible content of milk OBCFA in dairy sheep.
33069404	8	93	theme	15:0	1346:1349	arg1	anteiso					1351:1357	15:0 anteiso	1346:1357	15:0 anteiso	1346:1357	According to the loading plot, dietary unsaturated C18 FA loaded opposite to major milk OBCFA (e.g., 15:0, 15:0 anteiso, and 17:0) and were strongly correlated with principal component 1, which described 46% of variability.
33069404	0	94	theme	odd-	53:56	arg1	composition					88:98	milk odd- and branched-chain fatty acid composition	48:98	milk odd- and branched-chain fatty acid composition in dairy ewes	48:112	Effect of dietary lipids and other nutrients on milk odd- and branched-chain fatty acid composition in dairy ewes.
33069404	2	95	theme	other	444:448	arg1	nutrients					450:458	other nutrients	444:458	other nutrients	444:458	Targeted research has examined relationships between these fatty acids (FA) and dietary components, but interactions between the effects of lipids and other nutrients on milk OBCFA are not well characterized yet.
33069404	12	96	theme	acid	2113:2116	arg1	coefficients					2151:2162	positive linear coefficients	2135:2162	positive linear coefficients	2135:2162	In this regard, regression equations indicated that iso FA would show opposite responses to increasing levels of acid detergent fiber (positive linear coefficients) and starch (negative linear coefficients).
33069404	12	96	theme	acid	2113:2116	arg1	fiber					2128:2132	acid detergent fiber	2113:2132	acid detergent fiber (positive linear coefficients)	2113:2163	In this regard, regression equations indicated that iso FA would show opposite responses to increasing levels of acid detergent fiber (positive linear coefficients) and starch (negative linear coefficients).
33069404	7	97	theme	dietary	1132:1138	arg1	components					1140:1149	specific dietary components	1123:1149	specific dietary components (selected on the basis of the PCA)	1123:1184	Then, responses of milk OBCFA to variations in specific dietary components (selected on the basis of the PCA) were examined in more detail by regression analysis.
33069404	0	98	theme	branched-chain	62:75	arg1	composition					88:98	milk odd- and branched-chain fatty acid composition	48:98	milk odd- and branched-chain fatty acid composition in dairy ewes	48:112	Effect of dietary lipids and other nutrients on milk odd- and branched-chain fatty acid composition in dairy ewes.
33069404	1	99	theme	branched-chain	129:142	arg1	acids					150:154	Milk odd- and branched-chain fatty acids	115:154	acids	150:154	Milk odd- and branched-chain fatty acids (OBCFA) are largely derived from bacteria leaving the rumen, which has encouraged research on their use as biomarkers of rumen function.
33069404	2	100	theme	lipids	433:438	arg1	effects					422:428	the effects	418:428	the effects of lipids and other nutrients on milk OBCFA	418:472	Targeted research has examined relationships between these fatty acids (FA) and dietary components, but interactions between the effects of lipids and other nutrients on milk OBCFA are not well characterized yet.
33069404	12	101	theme	fiber	2128:2132	arg1	levels					2103:2108	increasing levels	2092:2108	increasing levels of acid detergent fiber (positive linear coefficients) and starch (negative linear coefficients)	2092:2205	In this regard, regression equations indicated that iso FA would show opposite responses to increasing levels of acid detergent fiber (positive linear coefficients) and starch (negative linear coefficients).
33069404	0	102	theme	acid	83:86	arg1	composition					88:98	milk odd- and branched-chain fatty acid composition	48:98	milk odd- and branched-chain fatty acid composition in dairy ewes	48:112	Effect of dietary lipids and other nutrients on milk odd- and branched-chain fatty acid composition in dairy ewes.
33069404	5	103	theme	basal	884:888	arg1	diets					890:894	the same basal diets	875:894	the same basal diets	875:894	A total of 47 lots received lipid supplements, whereas their respective controls (27 lots) were fed the same basal diets without lipid supplementation.
33069404	6	104	theme	component	1023:1031	arg1	PCA					1043:1045	PCA	1043:1045	PCA	1043:1045	Relationships between milk OBCFA and dietary components were first assessed through a principal component analysis (PCA) and a correlation analysis.
33069404	6	104	theme	component	1023:1031	arg1	analysis					1033:1040	a principal component analysis	1011:1040	a principal component analysis (PCA)	1011:1046	Relationships between milk OBCFA and dietary components were first assessed through a principal component analysis (PCA) and a correlation analysis.
33069404	8	105	theme	principal	1404:1412	arg1	component					1414:1422	principal component 1	1404:1424	principal component 1	1404:1424	According to the loading plot, dietary unsaturated C18 FA loaded opposite to major milk OBCFA (e.g., 15:0, 15:0 anteiso, and 17:0) and were strongly correlated with principal component 1, which described 46% of variability.
33069404	8	106	theme	loading	1256:1262	arg1	plot					1264:1267	the loading plot	1252:1267	the loading plot	1252:1267	According to the loading plot, dietary unsaturated C18 FA loaded opposite to major milk OBCFA (e.g., 15:0, 15:0 anteiso, and 17:0) and were strongly correlated with principal component 1, which described 46% of variability.
33069404	7	107	theme	OBCFA	1100:1104	arg1	responses					1082:1090	responses	1082:1090	responses of milk OBCFA to variations in specific dietary components (selected on the basis of the PCA)	1082:1184	Then, responses of milk OBCFA to variations in specific dietary components (selected on the basis of the PCA) were examined in more detail by regression analysis.
33069404	7	108	theme	regression	1218:1227	arg1	analysis					1229:1236	regression analysis	1218:1236	regression analysis	1218:1236	Then, responses of milk OBCFA to variations in specific dietary components (selected on the basis of the PCA) were examined in more detail by regression analysis.
33069404	9	109	dep	negative	1508:1515	arg1	linear					1532:1537	linear	1532:1537	linear	1532:1537	Overall, regression equations supported this negative, and generally linear, relationship between unsaturated C18 FA levels and milk OBCFA.
33069404	11	110	theme	bacteria	1990:1997	arg1	biomarkers					1942:1951	biomarkers	1942:1951	biomarkers of rumen cellulolytic and amylolytic bacteria	1942:1997	The PCA also suggested that dietary crude protein is not a determinant of milk OBCFA profile in dairy ewes, but significant relationships were observed between some OBCFA and dietary fiber or starch, consistent with a potential role of these FA as biomarkers of rumen cellulolytic and amylolytic bacteria.
33069404	8	111	theme	unsaturated	1278:1288	arg1	FA					1294:1295	dietary unsaturated C18 FA	1270:1295	dietary unsaturated C18 FA	1270:1295	According to the loading plot, dietary unsaturated C18 FA loaded opposite to major milk OBCFA (e.g., 15:0, 15:0 anteiso, and 17:0) and were strongly correlated with principal component 1, which described 46% of variability.
33069404	11	112	theme	profile	1779:1785	arg1	determinant					1753:1763	a determinant	1751:1763	a determinant of milk OBCFA profile in dairy ewes	1751:1799	The PCA also suggested that dietary crude protein is not a determinant of milk OBCFA profile in dairy ewes, but significant relationships were observed between some OBCFA and dietary fiber or starch, consistent with a potential role of these FA as biomarkers of rumen cellulolytic and amylolytic bacteria.
33069404	11	112	theme	profile	1779:1785	arg1	protein					1736:1742	dietary crude protein	1722:1742	dietary crude protein	1722:1742	The PCA also suggested that dietary crude protein is not a determinant of milk OBCFA profile in dairy ewes, but significant relationships were observed between some OBCFA and dietary fiber or starch, consistent with a potential role of these FA as biomarkers of rumen cellulolytic and amylolytic bacteria.
33069404	0	113	theme	other	29:33	arg1	nutrients					35:43	other nutrients	29:43	other nutrients	29:43	Effect of dietary lipids and other nutrients on milk odd- and branched-chain fatty acid composition in dairy ewes.
33069404	11	114	with	consistent	1894:1903	arg1	role					1922:1925	a potential role	1910:1925	a potential role of these FA as biomarkers of rumen cellulolytic and amylolytic bacteria	1910:1997	The PCA also suggested that dietary crude protein is not a determinant of milk OBCFA profile in dairy ewes, but significant relationships were observed between some OBCFA and dietary fiber or starch, consistent with a potential role of these FA as biomarkers of rumen cellulolytic and amylolytic bacteria.
33069404	14	115	theme	possible	2592:2599	arg1	content					2601:2607	the highest possible content	2580:2607	the highest possible content of milk OBCFA in dairy sheep	2580:2636	Because consumption of these FA may have nutritional benefits for humans, the use of high-fiber/low-starch rations might be recommended to maintain the highest possible content of milk OBCFA in dairy sheep.
33069404	4	116	theme	lipid	756:760	arg1	supplements					762:772	lipid supplements	756:772	lipid supplements	756:772	Thus, the present meta-analysis examined relationships between diet composition and milk OBCFA using a database compiled with lot observations from 14 trials in dairy ewes fed lipid supplements.
33069404	11	117	theme	milk	1768:1771	arg1	profile					1779:1785	milk OBCFA profile	1768:1785	milk OBCFA profile in dairy ewes	1768:1799	The PCA also suggested that dietary crude protein is not a determinant of milk OBCFA profile in dairy ewes, but significant relationships were observed between some OBCFA and dietary fiber or starch, consistent with a potential role of these FA as biomarkers of rumen cellulolytic and amylolytic bacteria.
33069404	8	118	dep	15:0	1340:1343	arg1	e.g.					1334:1337	e.g.	1334:1337	e.g.	1334:1337	According to the loading plot, dietary unsaturated C18 FA loaded opposite to major milk OBCFA (e.g., 15:0, 15:0 anteiso, and 17:0) and were strongly correlated with principal component 1, which described 46% of variability.
33069404	14	119	theme	rations	2539:2545	arg1	use					2510:2512	the use	2506:2512	the use of high-fiber/low-starch rations	2506:2545	Because consumption of these FA may have nutritional benefits for humans, the use of high-fiber/low-starch rations might be recommended to maintain the highest possible content of milk OBCFA in dairy sheep.
33069404	2	120	theme	Targeted	293:300	arg1	research					302:309	Targeted research	293:309	Targeted research	293:309	Targeted research has examined relationships between these fatty acids (FA) and dietary components, but interactions between the effects of lipids and other nutrients on milk OBCFA are not well characterized yet.
33069404	1	121	theme	rumen	277:281	arg1	function					283:290	rumen function	277:290	rumen function	277:290	Milk odd- and branched-chain fatty acids (OBCFA) are largely derived from bacteria leaving the rumen, which has encouraged research on their use as biomarkers of rumen function.
33069404	9	122	theme	regression	1472:1481	arg1	equations					1483:1491	regression equations	1472:1491	regression equations	1472:1491	Overall, regression equations supported this negative, and generally linear, relationship between unsaturated C18 FA levels and milk OBCFA.
33069404	10	123	theme	saturated	1663:1671	arg1	FA					1673:1674	saturated FA	1663:1674	saturated FA	1663:1674	However, the influence of C20-22 n-3 polyunsaturated FA and saturated FA was more limited.
33069404	7	124	theme	PCA	1181:1183	arg1	basis					1168:1172	the basis	1164:1172	the basis of the PCA	1164:1183	Then, responses of milk OBCFA to variations in specific dietary components (selected on the basis of the PCA) were examined in more detail by regression analysis.
33069404	8	125	theme	major	1316:1320	arg1	OBCFA					1327:1331	major milk OBCFA	1316:1331	major milk OBCFA (e.g., 15:0, 15:0 anteiso, and 17:0)	1316:1368	According to the loading plot, dietary unsaturated C18 FA loaded opposite to major milk OBCFA (e.g., 15:0, 15:0 anteiso, and 17:0) and were strongly correlated with principal component 1, which described 46% of variability.
33069404	14	126	theme	highest	2584:2590	arg1	content					2601:2607	the highest possible content	2580:2607	the highest possible content of milk OBCFA in dairy sheep	2580:2636	Because consumption of these FA may have nutritional benefits for humans, the use of high-fiber/low-starch rations might be recommended to maintain the highest possible content of milk OBCFA in dairy sheep.
32679299	2	0	with	women	370:374	arg1	cirrhosis					381:389	cirrhosis	381:389	cirrhosis	381:389	We aimed to determine differences in fecal microbiota composition/functionality between men and women with cirrhosis and HE on differing treatments.
32679299	2	0	with	women	370:374	arg1	HE					395:396	HE	395:396	HE	395:396	We aimed to determine differences in fecal microbiota composition/functionality between men and women with cirrhosis and HE on differing treatments.
32679299	3	1	theme	functional	512:521	arg1	analyses					523:530	METHODS Cross-sectional stool microbiome composition (16s rRNA sequencing) and microbial functional analyses	423:530	analyses	523:530	METHODS Cross-sectional stool microbiome composition (16s rRNA sequencing) and microbial functional analyses were performed in men and women with cirrhosis, and controls.
32679299	1	2	theme	sex	241:243	arg1	unclear					265:271	unclear	265:271	unclear	265:271	BACKGROUND & AIMS Altered microbiota can affect the gut-liver-brain axis in cirrhosis and hepatic encephalopathy (HE), but the impact of sex on these changes is unclear.
32679299	1	2	theme	sex	241:243	arg1	impact					231:236	the impact	227:236	the impact of sex on these changes	227:260	BACKGROUND & AIMS Altered microbiota can affect the gut-liver-brain axis in cirrhosis and hepatic encephalopathy (HE), but the impact of sex on these changes is unclear.
32679299	7	3	used	used	986:989	arg2	Men					951:953	Men	951:953	Men	951:953	Men were older and more frequently used proton pump inhibitors (PPIs), but model for end-stage liver disease score, No-HE (n = 319), HE-lac (n = 130) and HE-Rif (n = 170) proportions were similar.
32679299	2	4	from	differences	296:306	arg1	composition/functionality					328:352	fecal microbiota composition/functionality	311:352	fecal microbiota composition/functionality	311:352	We aimed to determine differences in fecal microbiota composition/functionality between men and women with cirrhosis and HE on differing treatments.
32679299	10	5	dep	PERMANOVA	1486:1494	arg1	analyses					1504:1511	analyses	1504:1511	analyses	1504:1511	Men vs. women were different in all cirrhosis, No-HE and HE-Lac but not HE-Rif on PERMANOVA and AUC analyses.
32679299	16	6	theme	sex-specific	2165:2176	arg1	outcomes					2178:2185	sex-specific outcomes	2165:2185	sex-specific outcomes	2165:2185	Further studies linking these differences to sex-specific outcomes are needed.
32679299	6	7	theme	individuals	847:857	arg1	total					834:838	A total	832:838	A total of 761 individuals	832:857	RESULTS A total of 761 individuals were included, 619 with cirrhosis (466 men, 153 women) and 142 controls (92 men, 50 women).
32679299	17	8	theme	brain	2260:2264	arg1	function					2266:2273	their brain function	2254:2273	their brain function	2254:2273	LAY SUMMARY Patients with cirrhosis develop changes in their brain function, and men often develop feminization with disease progression.
32679299	17	9	with	feminization	2298:2309	arg1	progression					2324:2334	disease progression	2316:2334	disease progression	2316:2334	LAY SUMMARY Patients with cirrhosis develop changes in their brain function, and men often develop feminization with disease progression.
32679299	7	10	theme	pump	998:1001	arg1	PPIs					1015:1018	PPIs	1015:1018	PPIs	1015:1018	Men were older and more frequently used proton pump inhibitors (PPIs), but model for end-stage liver disease score, No-HE (n = 319), HE-lac (n = 130) and HE-Rif (n = 170) proportions were similar.
32679299	7	10	theme	pump	998:1001	arg1	inhibitors					1003:1012	proton pump inhibitors	991:1012	proton pump inhibitors (PPIs)	991:1019	Men were older and more frequently used proton pump inhibitors (PPIs), but model for end-stage liver disease score, No-HE (n = 319), HE-lac (n = 130) and HE-Rif (n = 170) proportions were similar.
32679299	15	11	theme	microbial	1978:1986	arg1	function					1988:1995	gut microbial function	1974:1995	gut microbial function	1974:1995	CONCLUSIONS There are differences in gut microbial function and composition between men and women with cirrhosis, which could be implicated in differential responses to HE therapies.
32679299	13	12	theme	compound	1756:1763	arg1	degradation					1765:1775	glutamate and aromatic compound degradation	1733:1775	glutamate and aromatic compound degradation	1733:1775	Pathways related to glutamate and aromatic compound degradation were higher in men at all stages.
32679299	1	13	theme	hepatic	194:200	arg1	HE					218:219	HE	218:219	HE	218:219	BACKGROUND & AIMS Altered microbiota can affect the gut-liver-brain axis in cirrhosis and hepatic encephalopathy (HE), but the impact of sex on these changes is unclear.
32679299	1	13	theme	hepatic	194:200	arg1	encephalopathy					202:215	hepatic encephalopathy	194:215	hepatic encephalopathy (HE)	194:220	BACKGROUND & AIMS Altered microbiota can affect the gut-liver-brain axis in cirrhosis and hepatic encephalopathy (HE), but the impact of sex on these changes is unclear.
32679299	18	14	theme	disease	2390:2396	arg1	severity					2398:2405	disease severity	2390:2405	disease severity	2390:2405	However, the interaction between sex, microbiota and disease severity is unclear.
32679299	1	15	from	impact	231:236	arg1	changes					254:260	these changes	248:260	these changes	248:260	BACKGROUND & AIMS Altered microbiota can affect the gut-liver-brain axis in cirrhosis and hepatic encephalopathy (HE), but the impact of sex on these changes is unclear.
32679299	1	16	theme	Altered	122:128	arg1	microbiota					130:139	Altered microbiota	122:139	Altered microbiota	122:139	BACKGROUND & AIMS Altered microbiota can affect the gut-liver-brain axis in cirrhosis and hepatic encephalopathy (HE), but the impact of sex on these changes is unclear.
32679299	9	17	dep	Control	1343:1349	arg1	differentiation					1362:1376	differentiation	1362:1376	differentiation	1362:1376	Control vs. HE-Rif differentiation was similar across sexes.
32679299	13	18	theme	related	1722:1728	arg1	Pathways					1713:1720	Pathways	1713:1720	Pathways related to glutamate and aromatic compound degradation	1713:1775	Pathways related to glutamate and aromatic compound degradation were higher in men at all stages.
32679299	19	19	theme	hormone	2597:2603	arg1	metabolism					2605:2614	male hormone metabolism	2592:2614	male hormone metabolism	2592:2614	We found that as disease progressed in men, their microbial composition began to approach that observed in women, with changes in specific microbes that are associated with male hormone metabolism.
32679299	13	20	theme	glutamate	1733:1741	arg1	degradation					1765:1775	glutamate and aromatic compound degradation	1733:1775	glutamate and aromatic compound degradation	1733:1775	Pathways related to glutamate and aromatic compound degradation were higher in men at all stages.
32679299	0	21	theme	composition	52:62	arg1	and function					64:75	gut microbial composition and function	38:75	gut microbial composition and function in hepatic encephalopathy	38:101	Sex is associated with differences in gut microbial composition and function in hepatic encephalopathy.
32679299	17	22	from	changes	2243:2249	arg1	function					2266:2273	their brain function	2254:2273	their brain function	2254:2273	LAY SUMMARY Patients with cirrhosis develop changes in their brain function, and men often develop feminization with disease progression.
32679299	6	23	dep	controls	922:929	arg1	men					935:937	92 men	932:937	92 men	932:937	RESULTS A total of 761 individuals were included, 619 with cirrhosis (466 men, 153 women) and 142 controls (92 men, 50 women).
32679299	6	23	dep	controls	922:929	arg1	women					943:947	50 women	940:947	50 women	940:947	RESULTS A total of 761 individuals were included, 619 with cirrhosis (466 men, 153 women) and 142 controls (92 men, 50 women).
32679299	8	24	theme	differentiation	1172:1186	arg1	AUC					1165:1167	PPI/age-adjusted AUC	1148:1167	PPI/age-adjusted AUC of differentiation between controls vs. all cirrhosis, and controls vs. No-HE	1148:1245	PPI/age-adjusted AUC of differentiation between controls vs. all cirrhosis, and controls vs. No-HE were higher within women than men, but the adjusted AUC for No-HE vs. HE-Rif was higher in men.
32679299	3	25	with	controls	584:591	arg1	cirrhosis					569:577	cirrhosis	569:577	cirrhosis	569:577	METHODS Cross-sectional stool microbiome composition (16s rRNA sequencing) and microbial functional analyses were performed in men and women with cirrhosis, and controls.
32679299	14	26	theme	androstenedione	1826:1840	arg1	Degradation					1811:1821	Degradation	1811:1821	Degradation of androstenedione, an estrogenic precursor,	1811:1866	Degradation of androstenedione, an estrogenic precursor, was lower in men vs. women in HE-Rif, likely enhancing feminization.
32679299	15	27	with	men	2021:2023	arg1	cirrhosis					2040:2048	cirrhosis	2040:2048	cirrhosis	2040:2048	CONCLUSIONS There are differences in gut microbial function and composition between men and women with cirrhosis, which could be implicated in differential responses to HE therapies.
32679299	2	28	theme	differing	401:409	arg1	treatments					411:420	differing treatments	401:420	differing treatments	401:420	We aimed to determine differences in fecal microbiota composition/functionality between men and women with cirrhosis and HE on differing treatments.
32679299	3	29	theme	Cross-sectional	431:445	arg1	composition					464:474	METHODS Cross-sectional stool microbiome composition (16s rRNA sequencing) and microbial functional analyses	423:530	composition	464:474	METHODS Cross-sectional stool microbiome composition (16s rRNA sequencing) and microbial functional analyses were performed in men and women with cirrhosis, and controls.
32679299	0	30	from	differences	23:33	arg1	and function					64:75	gut microbial composition and function	38:75	gut microbial composition and function in hepatic encephalopathy	38:101	Sex is associated with differences in gut microbial composition and function in hepatic encephalopathy.
32679299	15	31	with	women	2029:2033	arg1	cirrhosis					2040:2048	cirrhosis	2040:2048	cirrhosis	2040:2048	CONCLUSIONS There are differences in gut microbial function and composition between men and women with cirrhosis, which could be implicated in differential responses to HE therapies.
32679299	8	32	theme	all	1209:1211	arg1	cirrhosis					1213:1221	all cirrhosis	1209:1221	all cirrhosis	1209:1221	PPI/age-adjusted AUC of differentiation between controls vs. all cirrhosis, and controls vs. No-HE were higher within women than men, but the adjusted AUC for No-HE vs. HE-Rif was higher in men.
32679299	17	33	theme	SUMMARY	2203:2209	arg1	Patients					2211:2218	LAY SUMMARY Patients	2199:2218	LAY SUMMARY Patients with cirrhosis	2199:2233	LAY SUMMARY Patients with cirrhosis develop changes in their brain function, and men often develop feminization with disease progression.
32679299	7	34	theme	liver	1046:1050	arg1	disease					1052:1058	end-stage liver disease	1036:1058	end-stage liver disease score	1036:1064	Men were older and more frequently used proton pump inhibitors (PPIs), but model for end-stage liver disease score, No-HE (n = 319), HE-lac (n = 130) and HE-Rif (n = 170) proportions were similar.
32679299	19	35	theme	microbial	2469:2477	arg1	composition					2479:2489	their microbial composition	2463:2489	their microbial composition	2463:2489	We found that as disease progressed in men, their microbial composition began to approach that observed in women, with changes in specific microbes that are associated with male hormone metabolism.
32679299	3	36	theme	microbiome	453:462	arg1	composition					464:474	METHODS Cross-sectional stool microbiome composition (16s rRNA sequencing) and microbial functional analyses	423:530	composition	464:474	METHODS Cross-sectional stool microbiome composition (16s rRNA sequencing) and microbial functional analyses were performed in men and women with cirrhosis, and controls.
32679299	19	37	theme	specific	2549:2556	arg1	microbes					2558:2565	specific microbes	2549:2565	specific microbes that are associated with male hormone metabolism	2549:2614	We found that as disease progressed in men, their microbial composition began to approach that observed in women, with changes in specific microbes that are associated with male hormone metabolism.
32679299	14	38	from	men	1881:1883	arg1	HE-Rif					1898:1903	HE-Rif	1898:1903	HE-Rif	1898:1903	Degradation of androstenedione, an estrogenic precursor, was lower in men vs. women in HE-Rif, likely enhancing feminization.
32679299	4	39	with	patients	644:651	arg1	HE-Lac					680:685	HE-Lac	680:685	HE-Lac	680:685	Patients with HE on rifaximin+lactulose (HE-Rif), patients with HE on lactulose only (HE-Lac) and those with cirrhosis without HE (No-HE) were compared to controls using random forest classifier.
32679299	4	39	with	patients	644:651	arg1	HE					608:609	HE	608:609	HE	608:609	Patients with HE on rifaximin+lactulose (HE-Rif), patients with HE on lactulose only (HE-Lac) and those with cirrhosis without HE (No-HE) were compared to controls using random forest classifier.
32679299	4	39	with	patients	644:651	arg1	on lactulose					661:672	HE on lactulose only	658:677	HE on lactulose only (HE-Lac)	658:686	Patients with HE on rifaximin+lactulose (HE-Rif), patients with HE on lactulose only (HE-Lac) and those with cirrhosis without HE (No-HE) were compared to controls using random forest classifier.
32679299	2	40	theme	microbiota	317:326	arg1	composition/functionality					328:352	fecal microbiota composition/functionality	311:352	fecal microbiota composition/functionality	311:352	We aimed to determine differences in fecal microbiota composition/functionality between men and women with cirrhosis and HE on differing treatments.
32679299	0	41	theme	gut	38:40	arg1	and function					64:75	gut microbial composition and function	38:75	gut microbial composition and function in hepatic encephalopathy	38:101	Sex is associated with differences in gut microbial composition and function in hepatic encephalopathy.
32679299	14	42	theme	estrogenic	1846:1855	arg1	precursor					1857:1865	an estrogenic precursor	1843:1865	an estrogenic precursor	1843:1865	Degradation of androstenedione, an estrogenic precursor, was lower in men vs. women in HE-Rif, likely enhancing feminization.
32679299	14	42	theme	estrogenic	1846:1855	arg1	androstenedione					1826:1840	androstenedione	1826:1840	androstenedione	1826:1840	Degradation of androstenedione, an estrogenic precursor, was lower in men vs. women in HE-Rif, likely enhancing feminization.
32679299	3	43	theme	rRNA	481:484	arg1	sequencing					486:495	16s rRNA sequencing	477:495	16s rRNA sequencing	477:495	METHODS Cross-sectional stool microbiome composition (16s rRNA sequencing) and microbial functional analyses were performed in men and women with cirrhosis, and controls.
32679299	3	44	dep	composition	464:474	arg1	sequencing					486:495	16s rRNA sequencing	477:495	16s rRNA sequencing	477:495	METHODS Cross-sectional stool microbiome composition (16s rRNA sequencing) and microbial functional analyses were performed in men and women with cirrhosis, and controls.
32679299	17	45	theme	disease	2316:2322	arg1	progression					2324:2334	disease progression	2316:2334	disease progression	2316:2334	LAY SUMMARY Patients with cirrhosis develop changes in their brain function, and men often develop feminization with disease progression.
32679299	4	46	theme	forest	771:776	arg1	classifier					778:787	random forest classifier	764:787	random forest classifier	764:787	Patients with HE on rifaximin+lactulose (HE-Rif), patients with HE on lactulose only (HE-Lac) and those with cirrhosis without HE (No-HE) were compared to controls using random forest classifier.
32679299	19	47	from	progressed	2444:2453	arg1	men					2458:2460	men	2458:2460	men	2458:2460	We found that as disease progressed in men, their microbial composition began to approach that observed in women, with changes in specific microbes that are associated with male hormone metabolism.
32679299	3	48	with	men	550:552	arg1	cirrhosis					569:577	cirrhosis	569:577	cirrhosis	569:577	METHODS Cross-sectional stool microbiome composition (16s rRNA sequencing) and microbial functional analyses were performed in men and women with cirrhosis, and controls.
32679299	10	49	dep	different	1423:1431	arg1	HE-Rif					1476:1481	HE-Rif	1476:1481	HE-Rif	1476:1481	Men vs. women were different in all cirrhosis, No-HE and HE-Lac but not HE-Rif on PERMANOVA and AUC analyses.
32679299	3	50	theme	microbial	502:510	arg1	analyses					523:530	METHODS Cross-sectional stool microbiome composition (16s rRNA sequencing) and microbial functional analyses	423:530	analyses	523:530	METHODS Cross-sectional stool microbiome composition (16s rRNA sequencing) and microbial functional analyses were performed in men and women with cirrhosis, and controls.
32679299	15	51	theme	HE	2106:2107	arg1	therapies					2109:2117	HE therapies	2106:2117	HE therapies	2106:2117	CONCLUSIONS There are differences in gut microbial function and composition between men and women with cirrhosis, which could be implicated in differential responses to HE therapies.
32679299	10	52	from	different	1423:1431	arg1	No-HE					1451:1455	No-HE	1451:1455	No-HE	1451:1455	Men vs. women were different in all cirrhosis, No-HE and HE-Lac but not HE-Rif on PERMANOVA and AUC analyses.
32679299	10	52	from	different	1423:1431	arg1	cirrhosis					1440:1448	all cirrhosis	1436:1448	all cirrhosis	1436:1448	Men vs. women were different in all cirrhosis, No-HE and HE-Lac but not HE-Rif on PERMANOVA and AUC analyses.
32679299	10	52	from	different	1423:1431	arg1	HE-Lac					1461:1466	HE-Lac	1461:1466	HE-Lac	1461:1466	Men vs. women were different in all cirrhosis, No-HE and HE-Lac but not HE-Rif on PERMANOVA and AUC analyses.
32679299	6	53	dep	619	874:876	arg1	men					898:900	466 men	894:900	466 men	894:900	RESULTS A total of 761 individuals were included, 619 with cirrhosis (466 men, 153 women) and 142 controls (92 men, 50 women).
32679299	6	53	dep	619	874:876	arg1	women					907:911	153 women	903:911	153 women	903:911	RESULTS A total of 761 individuals were included, 619 with cirrhosis (466 men, 153 women) and 142 controls (92 men, 50 women).
32679299	7	54	theme	proton	991:996	arg1	PPIs					1015:1018	PPIs	1015:1018	PPIs	1015:1018	Men were older and more frequently used proton pump inhibitors (PPIs), but model for end-stage liver disease score, No-HE (n = 319), HE-lac (n = 130) and HE-Rif (n = 170) proportions were similar.
32679299	7	54	theme	proton	991:996	arg1	inhibitors					1003:1012	proton pump inhibitors	991:1012	proton pump inhibitors (PPIs)	991:1019	Men were older and more frequently used proton pump inhibitors (PPIs), but model for end-stage liver disease score, No-HE (n = 319), HE-lac (n = 130) and HE-Rif (n = 170) proportions were similar.
32679299	15	55	theme	gut	1974:1976	arg1	function					1988:1995	gut microbial function	1974:1995	gut microbial function	1974:1995	CONCLUSIONS There are differences in gut microbial function and composition between men and women with cirrhosis, which could be implicated in differential responses to HE therapies.
32679299	17	56	with	Patients	2211:2218	arg1	cirrhosis					2225:2233	cirrhosis	2225:2233	cirrhosis	2225:2233	LAY SUMMARY Patients with cirrhosis develop changes in their brain function, and men often develop feminization with disease progression.
32679299	13	57	theme	aromatic	1747:1754	arg1	degradation					1765:1775	glutamate and aromatic compound degradation	1733:1775	glutamate and aromatic compound degradation	1733:1775	Pathways related to glutamate and aromatic compound degradation were higher in men at all stages.
32679299	4	58	from	patients	644:651	arg1	HE-Rif					635:640	HE-Rif	635:640	HE-Rif	635:640	Patients with HE on rifaximin+lactulose (HE-Rif), patients with HE on lactulose only (HE-Lac) and those with cirrhosis without HE (No-HE) were compared to controls using random forest classifier.
32679299	4	58	from	patients	644:651	arg1	rifaximin+lactulose					614:632	rifaximin+lactulose	614:632	rifaximin+lactulose (HE-Rif)	614:641	Patients with HE on rifaximin+lactulose (HE-Rif), patients with HE on lactulose only (HE-Lac) and those with cirrhosis without HE (No-HE) were compared to controls using random forest classifier.
32679299	10	59	theme	all	1436:1438	arg1	cirrhosis					1440:1448	all cirrhosis	1436:1448	all cirrhosis	1436:1448	Men vs. women were different in all cirrhosis, No-HE and HE-Lac but not HE-Rif on PERMANOVA and AUC analyses.
32679299	0	60	from	and function	64:75	arg1	encephalopathy					88:101	hepatic encephalopathy	80:101	hepatic encephalopathy	80:101	Sex is associated with differences in gut microbial composition and function in hepatic encephalopathy.
32679299	8	61	theme	adjusted	1290:1297	arg1	AUC					1299:1301	the adjusted AUC	1286:1301	the adjusted AUC for No-HE vs. HE-Rif	1286:1322	PPI/age-adjusted AUC of differentiation between controls vs. all cirrhosis, and controls vs. No-HE were higher within women than men, but the adjusted AUC for No-HE vs. HE-Rif was higher in men.
32679299	8	61	theme	adjusted	1290:1297	arg1	higher					1328:1333	higher	1328:1333	higher	1328:1333	PPI/age-adjusted AUC of differentiation between controls vs. all cirrhosis, and controls vs. No-HE were higher within women than men, but the adjusted AUC for No-HE vs. HE-Rif was higher in men.
32679299	4	62	with	Patients	594:601	arg1	HE-Lac					680:685	HE-Lac	680:685	HE-Lac	680:685	Patients with HE on rifaximin+lactulose (HE-Rif), patients with HE on lactulose only (HE-Lac) and those with cirrhosis without HE (No-HE) were compared to controls using random forest classifier.
32679299	4	62	with	Patients	594:601	arg1	HE					608:609	HE	608:609	HE	608:609	Patients with HE on rifaximin+lactulose (HE-Rif), patients with HE on lactulose only (HE-Lac) and those with cirrhosis without HE (No-HE) were compared to controls using random forest classifier.
32679299	4	62	with	Patients	594:601	arg1	on lactulose					661:672	HE on lactulose only	658:677	HE on lactulose only (HE-Lac)	658:686	Patients with HE on rifaximin+lactulose (HE-Rif), patients with HE on lactulose only (HE-Lac) and those with cirrhosis without HE (No-HE) were compared to controls using random forest classifier.
32679299	19	63	theme	male	2592:2595	arg1	metabolism					2605:2614	male hormone metabolism	2592:2614	male hormone metabolism	2592:2614	We found that as disease progressed in men, their microbial composition began to approach that observed in women, with changes in specific microbes that are associated with male hormone metabolism.
32679299	10	64	from	No-HE	1451:1455	arg1	different					1423:1431	different	1423:1431	different	1423:1431	Men vs. women were different in all cirrhosis, No-HE and HE-Lac but not HE-Rif on PERMANOVA and AUC analyses.
32679299	15	65	dep	CONCLUSIONS	1937:1947	arg1	are					1955:1957	are	1955:1957	are differences in gut microbial function and composition between men and women with cirrhosis, which could be implicated in differential responses to HE therapies	1955:2117	CONCLUSIONS There are differences in gut microbial function and composition between men and women with cirrhosis, which could be implicated in differential responses to HE therapies.
32679299	15	66	from	differences	1959:1969	arg1	composition					2001:2011	composition	2001:2011	composition	2001:2011	CONCLUSIONS There are differences in gut microbial function and composition between men and women with cirrhosis, which could be implicated in differential responses to HE therapies.
32679299	15	66	from	differences	1959:1969	arg1	function					1988:1995	gut microbial function	1974:1995	gut microbial function	1974:1995	CONCLUSIONS There are differences in gut microbial function and composition between men and women with cirrhosis, which could be implicated in differential responses to HE therapies.
32679299	16	67	theme	Further	2120:2126	arg1	studies					2128:2134	Further studies	2120:2134	Further studies linking these differences to sex-specific outcomes	2120:2185	Further studies linking these differences to sex-specific outcomes are needed.
32679299	0	68	theme	hepatic	80:86	arg1	encephalopathy					88:101	hepatic encephalopathy	80:101	hepatic encephalopathy	80:101	Sex is associated with differences in gut microbial composition and function in hepatic encephalopathy.
32679299	1	69	theme	gut-liver-brain	156:170	arg1	axis					172:175	the gut-liver-brain axis	152:175	the gut-liver-brain axis in cirrhosis and hepatic encephalopathy (HE)	152:220	BACKGROUND & AIMS Altered microbiota can affect the gut-liver-brain axis in cirrhosis and hepatic encephalopathy (HE), but the impact of sex on these changes is unclear.
32679299	8	70	theme	PPI/age-adjusted	1148:1163	arg1	AUC					1165:1167	PPI/age-adjusted AUC	1148:1167	PPI/age-adjusted AUC of differentiation between controls vs. all cirrhosis, and controls vs. No-HE	1148:1245	PPI/age-adjusted AUC of differentiation between controls vs. all cirrhosis, and controls vs. No-HE were higher within women than men, but the adjusted AUC for No-HE vs. HE-Rif was higher in men.
32679299	4	71	from	Patients	594:601	arg1	HE-Rif					635:640	HE-Rif	635:640	HE-Rif	635:640	Patients with HE on rifaximin+lactulose (HE-Rif), patients with HE on lactulose only (HE-Lac) and those with cirrhosis without HE (No-HE) were compared to controls using random forest classifier.
32679299	4	71	from	Patients	594:601	arg1	rifaximin+lactulose					614:632	rifaximin+lactulose	614:632	rifaximin+lactulose (HE-Rif)	614:641	Patients with HE on rifaximin+lactulose (HE-Rif), patients with HE on lactulose only (HE-Lac) and those with cirrhosis without HE (No-HE) were compared to controls using random forest classifier.
32679299	1	72	from	axis	172:175	arg1	HE					218:219	HE	218:219	HE	218:219	BACKGROUND & AIMS Altered microbiota can affect the gut-liver-brain axis in cirrhosis and hepatic encephalopathy (HE), but the impact of sex on these changes is unclear.
32679299	1	72	from	axis	172:175	arg1	cirrhosis					180:188	cirrhosis	180:188	cirrhosis	180:188	BACKGROUND & AIMS Altered microbiota can affect the gut-liver-brain axis in cirrhosis and hepatic encephalopathy (HE), but the impact of sex on these changes is unclear.
32679299	1	72	from	axis	172:175	arg1	encephalopathy					202:215	hepatic encephalopathy	194:215	hepatic encephalopathy (HE)	194:220	BACKGROUND & AIMS Altered microbiota can affect the gut-liver-brain axis in cirrhosis and hepatic encephalopathy (HE), but the impact of sex on these changes is unclear.
32679299	11	73	theme	disease	1574:1580	arg1	progression					1582:1592	disease progression	1574:1592	disease progression	1574:1592	Autochthonous taxa decreased and pathobionts increased with disease progression regardless of sex.
32679299	19	74	theme	disease	2436:2442	arg1	progressed					2444:2453	disease progressed	2436:2453	disease progressed in men	2436:2460	We found that as disease progressed in men, their microbial composition began to approach that observed in women, with changes in specific microbes that are associated with male hormone metabolism.
32679299	3	75	theme	stool	447:451	arg1	composition					464:474	METHODS Cross-sectional stool microbiome composition (16s rRNA sequencing) and microbial functional analyses	423:530	composition	464:474	METHODS Cross-sectional stool microbiome composition (16s rRNA sequencing) and microbial functional analyses were performed in men and women with cirrhosis, and controls.
32679299	3	76	with	women	558:562	arg1	cirrhosis					569:577	cirrhosis	569:577	cirrhosis	569:577	METHODS Cross-sectional stool microbiome composition (16s rRNA sequencing) and microbial functional analyses were performed in men and women with cirrhosis, and controls.
32679299	17	77	theme	LAY	2199:2201	arg1	Patients					2211:2218	LAY SUMMARY Patients	2199:2218	LAY SUMMARY Patients with cirrhosis	2199:2233	LAY SUMMARY Patients with cirrhosis develop changes in their brain function, and men often develop feminization with disease progression.
32679299	3	78	theme	METHODS	423:429	arg1	composition					464:474	METHODS Cross-sectional stool microbiome composition (16s rRNA sequencing) and microbial functional analyses	423:530	composition	464:474	METHODS Cross-sectional stool microbiome composition (16s rRNA sequencing) and microbial functional analyses were performed in men and women with cirrhosis, and controls.
32679299	7	79	theme	end-stage	1036:1044	arg1	disease					1052:1058	end-stage liver disease	1036:1058	end-stage liver disease score	1036:1064	Men were older and more frequently used proton pump inhibitors (PPIs), but model for end-stage liver disease score, No-HE (n = 319), HE-lac (n = 130) and HE-Rif (n = 170) proportions were similar.
32679299	15	80	theme	differential	2080:2091	arg1	responses					2093:2101	differential responses	2080:2101	differential responses to HE therapies	2080:2117	CONCLUSIONS There are differences in gut microbial function and composition between men and women with cirrhosis, which could be implicated in differential responses to HE therapies.
32679299	1	81	theme	BACKGROUND	104:113	arg1	&					115:115	BACKGROUND &	104:115	BACKGROUND &	104:115	BACKGROUND & AIMS Altered microbiota can affect the gut-liver-brain axis in cirrhosis and hepatic encephalopathy (HE), but the impact of sex on these changes is unclear.
32679299	2	82	from	HE	395:396	arg1	treatments					411:420	differing treatments	401:420	differing treatments	401:420	We aimed to determine differences in fecal microbiota composition/functionality between men and women with cirrhosis and HE on differing treatments.
32679299	3	83	theme	16s	477:479	arg1	sequencing					486:495	16s rRNA sequencing	477:495	16s rRNA sequencing	477:495	METHODS Cross-sectional stool microbiome composition (16s rRNA sequencing) and microbial functional analyses were performed in men and women with cirrhosis, and controls.
32679299	7	84	theme	model	1026:1030	arg1	proportions					1122:1132	model for end-stage liver disease score, No-HE (n = 319), HE-lac (n = 130) and HE-Rif (n = 170) proportions	1026:1132	model for end-stage liver disease score, No-HE (n = 319), HE-lac (n = 130) and HE-Rif (n = 170) proportions	1026:1132	Men were older and more frequently used proton pump inhibitors (PPIs), but model for end-stage liver disease score, No-HE (n = 319), HE-lac (n = 130) and HE-Rif (n = 170) proportions were similar.
32679299	6	85	dep	RESULTS	824:830	arg1	included					864:871	included	864:871	included	864:871	RESULTS A total of 761 individuals were included, 619 with cirrhosis (466 men, 153 women) and 142 controls (92 men, 50 women).
32679299	0	86	theme	microbial	42:50	arg1	and function					64:75	gut microbial composition and function	38:75	gut microbial composition and function in hepatic encephalopathy	38:101	Sex is associated with differences in gut microbial composition and function in hepatic encephalopathy.
32679299	10	87	from	cirrhosis	1440:1448	arg1	different					1423:1431	different	1423:1431	different	1423:1431	Men vs. women were different in all cirrhosis, No-HE and HE-Lac but not HE-Rif on PERMANOVA and AUC analyses.
32679299	11	88	theme	Autochthonous	1514:1526	arg1	taxa					1528:1531	Autochthonous taxa	1514:1531	Autochthonous taxa	1514:1531	Autochthonous taxa decreased and pathobionts increased with disease progression regardless of sex.
32679299	2	89	theme	fecal	311:315	arg1	composition/functionality					328:352	fecal microbiota composition/functionality	311:352	fecal microbiota composition/functionality	311:352	We aimed to determine differences in fecal microbiota composition/functionality between men and women with cirrhosis and HE on differing treatments.
32679299	2	90	from	cirrhosis	381:389	arg1	treatments					411:420	differing treatments	401:420	differing treatments	401:420	We aimed to determine differences in fecal microbiota composition/functionality between men and women with cirrhosis and HE on differing treatments.
32679299	19	91	from	changes	2538:2544	arg1	microbes					2558:2565	specific microbes	2549:2565	specific microbes that are associated with male hormone metabolism	2549:2614	We found that as disease progressed in men, their microbial composition began to approach that observed in women, with changes in specific microbes that are associated with male hormone metabolism.
32679299	2	92	with	men	362:364	arg1	cirrhosis					381:389	cirrhosis	381:389	cirrhosis	381:389	We aimed to determine differences in fecal microbiota composition/functionality between men and women with cirrhosis and HE on differing treatments.
32679299	2	92	with	men	362:364	arg1	HE					395:396	HE	395:396	HE	395:396	We aimed to determine differences in fecal microbiota composition/functionality between men and women with cirrhosis and HE on differing treatments.
32679299	10	93	from	HE-Lac	1461:1466	arg1	different					1423:1431	different	1423:1431	different	1423:1431	Men vs. women were different in all cirrhosis, No-HE and HE-Lac but not HE-Rif on PERMANOVA and AUC analyses.
32679299	4	94	theme	random	764:769	arg1	classifier					778:787	random forest classifier	764:787	random forest classifier	764:787	Patients with HE on rifaximin+lactulose (HE-Rif), patients with HE on lactulose only (HE-Lac) and those with cirrhosis without HE (No-HE) were compared to controls using random forest classifier.
32679299	7	95	theme	disease	1052:1058	arg1	score					1060:1064	end-stage liver disease score	1036:1064	end-stage liver disease score	1036:1064	Men were older and more frequently used proton pump inhibitors (PPIs), but model for end-stage liver disease score, No-HE (n = 319), HE-lac (n = 130) and HE-Rif (n = 170) proportions were similar.
34959902	7	0	theme	function	1508:1515	arg1	composition					1550:1560	the composition	1546:1560	the composition of the intestinal microbiome	1546:1589	These results suggest that feeding an FV-supplemented diet for two weeks modulated markers of cellular inflammatory and immune function in the WBC transcriptome and the composition of the intestinal microbiome by increasing the abundance of bacterial taxa that have been associated with improved intestinal health.
34959902	7	0	theme	function	1508:1515	arg1	markers					1464:1470	markers	1464:1470	markers of cellular inflammatory and immune function in the WBC transcriptome	1464:1540	These results suggest that feeding an FV-supplemented diet for two weeks modulated markers of cellular inflammatory and immune function in the WBC transcriptome and the composition of the intestinal microbiome by increasing the abundance of bacterial taxa that have been associated with improved intestinal health.
34959902	6	1	theme	fecal	1055:1059	arg1	samples					1072:1078	fecal microbiome samples	1055:1078	fecal microbiome samples	1055:1078	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples showed differential increases in genera from Lachnospiraceae and Ruminococcaceae families within the order Clostridiales and Erysipelotrichaceae family with a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet.
34959902	7	2	theme	bacterial	1622:1630	arg1	taxa					1632:1635	bacterial taxa	1622:1635	bacterial taxa that have been associated with improved intestinal health	1622:1693	These results suggest that feeding an FV-supplemented diet for two weeks modulated markers of cellular inflammatory and immune function in the WBC transcriptome and the composition of the intestinal microbiome by increasing the abundance of bacterial taxa that have been associated with improved intestinal health.
34959902	7	3	from	composition	1550:1560	arg1	transcriptome					1528:1540	the WBC transcriptome	1520:1540	the WBC transcriptome	1520:1540	These results suggest that feeding an FV-supplemented diet for two weeks modulated markers of cellular inflammatory and immune function in the WBC transcriptome and the composition of the intestinal microbiome by increasing the abundance of bacterial taxa that have been associated with improved intestinal health.
34959902	7	4	theme	WBC	1524:1526	arg1	transcriptome					1528:1540	the WBC transcriptome	1520:1540	the WBC transcriptome	1520:1540	These results suggest that feeding an FV-supplemented diet for two weeks modulated markers of cellular inflammatory and immune function in the WBC transcriptome and the composition of the intestinal microbiome by increasing the abundance of bacterial taxa that have been associated with improved intestinal health.
34959902	6	5	theme	lipopolysaccharide	1311:1328	arg1	biosynthesis					1330:1341	lipopolysaccharide biosynthesis	1311:1341	lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet	1311:1378	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples showed differential increases in genera from Lachnospiraceae and Ruminococcaceae families within the order Clostridiales and Erysipelotrichaceae family with a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet.
34959902	4	6	theme	Isolated	632:639	arg1	DNA					641:643	Isolated DNA	632:643	Isolated DNA from the fecal microbiome	632:669	Isolated DNA from the fecal microbiome was used for 16S rDNA taxonomic analysis and prediction of metabolomic function.
34959902	5	7	from	regulation	919:928	arg1	differentiation					895:909	differentiation	895:909	differentiation	895:909	Feeding an FV-supplemented diet to pigs induced differential expression of several genes associated with an increase in B-cell development and differentiation and the regulation of cellular movement, inflammatory response, and cell-to-cell signaling.
34959902	5	7	from	regulation	919:928	arg1	development					879:889	B-cell development	872:889	B-cell development	872:889	Feeding an FV-supplemented diet to pigs induced differential expression of several genes associated with an increase in B-cell development and differentiation and the regulation of cellular movement, inflammatory response, and cell-to-cell signaling.
34959902	6	8	from	reduction	1249:1257	arg1	protein					1287:1293	rgpE-glucosyltransferase protein	1262:1293	rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet	1262:1378	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples showed differential increases in genera from Lachnospiraceae and Ruminococcaceae families within the order Clostridiales and Erysipelotrichaceae family with a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet.
34959902	0	9	theme	Two-Week	93:100	arg1	Intervention					110:121	a Two-Week Feeding Intervention	91:121	a Two-Week Feeding Intervention	91:121	Fruit and Vegetable Supplemented Diet Modulates the Pig Transcriptome and Microbiome after a Two-Week Feeding Intervention.
34959902	5	10	theme	inflammatory	952:963	arg1	response					965:972	inflammatory response	952:972	inflammatory response	952:972	Feeding an FV-supplemented diet to pigs induced differential expression of several genes associated with an increase in B-cell development and differentiation and the regulation of cellular movement, inflammatory response, and cell-to-cell signaling.
34959902	6	11	from	size	1039:1042	arg1	samples					1072:1078	fecal microbiome samples	1055:1078	fecal microbiome samples	1055:1078	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples showed differential increases in genera from Lachnospiraceae and Ruminococcaceae families within the order Clostridiales and Erysipelotrichaceae family with a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet.
34959902	4	12	theme	taxonomic	693:701	arg1	analysis					703:710	rDNA taxonomic analysis	688:710	rDNA taxonomic analysis	688:710	Isolated DNA from the fecal microbiome was used for 16S rDNA taxonomic analysis and prediction of metabolomic function.
34959902	4	13	used	used	675:678	arg2	DNA					641:643	Isolated DNA	632:643	Isolated DNA from the fecal microbiome	632:669	Isolated DNA from the fecal microbiome was used for 16S rDNA taxonomic analysis and prediction of metabolomic function.
34959902	1	14	theme	host	233:236	arg1	WBC					256:258	WBC	256:258	WBC	256:258	A study was conducted to determine the effects of a diet supplemented with fruits and vegetables (FV) on the host whole blood cell (WBC) transcriptome and the composition and function of the intestinal microbiome.
34959902	1	14	theme	host	233:236	arg1	cell					250:253	host whole blood cell	233:253	the host whole blood cell (WBC) transcriptome	229:273	A study was conducted to determine the effects of a diet supplemented with fruits and vegetables (FV) on the host whole blood cell (WBC) transcriptome and the composition and function of the intestinal microbiome.
34959902	4	15	theme	rDNA	688:691	arg1	analysis					703:710	rDNA taxonomic analysis	688:710	rDNA taxonomic analysis	688:710	Isolated DNA from the fecal microbiome was used for 16S rDNA taxonomic analysis and prediction of metabolomic function.
34959902	6	16	dep	Lachnospiraceae	1125:1139	arg1	families					1161:1168	families	1161:1168	families	1161:1168	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples showed differential increases in genera from Lachnospiraceae and Ruminococcaceae families within the order Clostridiales and Erysipelotrichaceae family with a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet.
34959902	1	17	theme	whole	238:242	arg1	WBC					256:258	WBC	256:258	WBC	256:258	A study was conducted to determine the effects of a diet supplemented with fruits and vegetables (FV) on the host whole blood cell (WBC) transcriptome and the composition and function of the intestinal microbiome.
34959902	1	17	theme	whole	238:242	arg1	cell					250:253	host whole blood cell	233:253	the host whole blood cell (WBC) transcriptome	229:273	A study was conducted to determine the effects of a diet supplemented with fruits and vegetables (FV) on the host whole blood cell (WBC) transcriptome and the composition and function of the intestinal microbiome.
34959902	1	18	theme	intestinal	315:324	arg1	microbiome					326:335	the intestinal microbiome	311:335	the intestinal microbiome	311:335	A study was conducted to determine the effects of a diet supplemented with fruits and vegetables (FV) on the host whole blood cell (WBC) transcriptome and the composition and function of the intestinal microbiome.
34959902	6	19	theme	analysis	1023:1030	arg1	LEfSe					1045:1049	LEfSe	1045:1049	LEfSe	1045:1049	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples showed differential increases in genera from Lachnospiraceae and Ruminococcaceae families within the order Clostridiales and Erysipelotrichaceae family with a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet.
34959902	6	19	theme	analysis	1023:1030	arg1	size					1039:1042	Linear discriminant analysis effect size	1003:1042	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples	1003:1078	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples showed differential increases in genera from Lachnospiraceae and Ruminococcaceae families within the order Clostridiales and Erysipelotrichaceae family with a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet.
34959902	5	20	theme	cellular	933:940	arg1	movement					942:949	cellular movement	933:949	cellular movement	933:949	Feeding an FV-supplemented diet to pigs induced differential expression of several genes associated with an increase in B-cell development and differentiation and the regulation of cellular movement, inflammatory response, and cell-to-cell signaling.
34959902	6	21	from	Ruminococcaceae	1145:1159	arg1	genera					1113:1118	genera	1113:1118	genera from Lachnospiraceae and Ruminococcaceae families	1113:1168	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples showed differential increases in genera from Lachnospiraceae and Ruminococcaceae families within the order Clostridiales and Erysipelotrichaceae family with a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet.
34959902	6	21	from	Ruminococcaceae	1145:1159	arg1	increases					1100:1108	differential increases	1087:1108	differential increases in genera from Lachnospiraceae and Ruminococcaceae families	1087:1168	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples showed differential increases in genera from Lachnospiraceae and Ruminococcaceae families within the order Clostridiales and Erysipelotrichaceae family with a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet.
34959902	5	22	theme	differential	800:811	arg1	expression					813:822	differential expression	800:822	differential expression of several genes associated with an increase in B-cell development and differentiation and the regulation of cellular movement, inflammatory response, and cell-to-cell signaling	800:1000	Feeding an FV-supplemented diet to pigs induced differential expression of several genes associated with an increase in B-cell development and differentiation and the regulation of cellular movement, inflammatory response, and cell-to-cell signaling.
34959902	0	23	theme	Feeding	102:108	arg1	Intervention					110:121	a Two-Week Feeding Intervention	91:121	a Two-Week Feeding Intervention	91:121	Fruit and Vegetable Supplemented Diet Modulates the Pig Transcriptome and Microbiome after a Two-Week Feeding Intervention.
34959902	1	24	theme	microbiome	326:335	arg1	function					299:306	function	299:306	function	299:306	A study was conducted to determine the effects of a diet supplemented with fruits and vegetables (FV) on the host whole blood cell (WBC) transcriptome and the composition and function of the intestinal microbiome.
34959902	1	24	theme	microbiome	326:335	arg1	transcriptome					261:273	the host whole blood cell (WBC) transcriptome	229:273	the host whole blood cell (WBC) transcriptome	229:273	A study was conducted to determine the effects of a diet supplemented with fruits and vegetables (FV) on the host whole blood cell (WBC) transcriptome and the composition and function of the intestinal microbiome.
34959902	1	24	theme	microbiome	326:335	arg1	composition					283:293	composition	283:293	composition	283:293	A study was conducted to determine the effects of a diet supplemented with fruits and vegetables (FV) on the host whole blood cell (WBC) transcriptome and the composition and function of the intestinal microbiome.
34959902	6	25	theme	rgpE-glucosyltransferase	1262:1285	arg1	protein					1287:1293	rgpE-glucosyltransferase protein	1262:1293	rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet	1262:1378	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples showed differential increases in genera from Lachnospiraceae and Ruminococcaceae families within the order Clostridiales and Erysipelotrichaceae family with a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet.
34959902	5	26	theme	movement	942:949	arg1	regulation					919:928	the regulation	915:928	the regulation of cellular movement, inflammatory response, and cell-to-cell signaling	915:1000	Feeding an FV-supplemented diet to pigs induced differential expression of several genes associated with an increase in B-cell development and differentiation and the regulation of cellular movement, inflammatory response, and cell-to-cell signaling.
34959902	5	26	theme	movement	942:949	arg1	increase					860:867	an increase	857:867	an increase in B-cell development and differentiation	857:909	Feeding an FV-supplemented diet to pigs induced differential expression of several genes associated with an increase in B-cell development and differentiation and the regulation of cellular movement, inflammatory response, and cell-to-cell signaling.
34959902	6	27	theme	Linear	1003:1008	arg1	analysis					1023:1030	Linear discriminant analysis	1003:1030	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples	1003:1078	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples showed differential increases in genera from Lachnospiraceae and Ruminococcaceae families within the order Clostridiales and Erysipelotrichaceae family with a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet.
34959902	5	28	theme	response	965:972	arg1	regulation					919:928	the regulation	915:928	the regulation of cellular movement, inflammatory response, and cell-to-cell signaling	915:1000	Feeding an FV-supplemented diet to pigs induced differential expression of several genes associated with an increase in B-cell development and differentiation and the regulation of cellular movement, inflammatory response, and cell-to-cell signaling.
34959902	5	28	theme	response	965:972	arg1	increase					860:867	an increase	857:867	an increase in B-cell development and differentiation	857:909	Feeding an FV-supplemented diet to pigs induced differential expression of several genes associated with an increase in B-cell development and differentiation and the regulation of cellular movement, inflammatory response, and cell-to-cell signaling.
34959902	2	29	theme	six-week-old	343:354	arg1	pigs					356:359	Nine six-week-old pigs	338:359	Nine six-week-old pigs	338:359	Nine six-week-old pigs were fed a pig grower diet alone or supplemented with lyophilized FV equivalent to half the daily recommended amount prescribed for humans by the Dietary Guideline for Americans (DGA) for two weeks.
34959902	7	30	from	function	1508:1515	arg1	transcriptome					1528:1540	the WBC transcriptome	1520:1540	the WBC transcriptome	1520:1540	These results suggest that feeding an FV-supplemented diet for two weeks modulated markers of cellular inflammatory and immune function in the WBC transcriptome and the composition of the intestinal microbiome by increasing the abundance of bacterial taxa that have been associated with improved intestinal health.
34959902	1	31	theme	blood	244:248	arg1	WBC					256:258	WBC	256:258	WBC	256:258	A study was conducted to determine the effects of a diet supplemented with fruits and vegetables (FV) on the host whole blood cell (WBC) transcriptome and the composition and function of the intestinal microbiome.
34959902	1	31	theme	blood	244:248	arg1	cell					250:253	host whole blood cell	233:253	the host whole blood cell (WBC) transcriptome	229:273	A study was conducted to determine the effects of a diet supplemented with fruits and vegetables (FV) on the host whole blood cell (WBC) transcriptome and the composition and function of the intestinal microbiome.
34959902	5	32	from	increase	860:867	arg1	differentiation					895:909	differentiation	895:909	differentiation	895:909	Feeding an FV-supplemented diet to pigs induced differential expression of several genes associated with an increase in B-cell development and differentiation and the regulation of cellular movement, inflammatory response, and cell-to-cell signaling.
34959902	5	32	from	increase	860:867	arg1	development					879:889	B-cell development	872:889	B-cell development	872:889	Feeding an FV-supplemented diet to pigs induced differential expression of several genes associated with an increase in B-cell development and differentiation and the regulation of cellular movement, inflammatory response, and cell-to-cell signaling.
34959902	7	33	theme	improved	1668:1675	arg1	health					1688:1693	improved intestinal health	1668:1693	improved intestinal health	1668:1693	These results suggest that feeding an FV-supplemented diet for two weeks modulated markers of cellular inflammatory and immune function in the WBC transcriptome and the composition of the intestinal microbiome by increasing the abundance of bacterial taxa that have been associated with improved intestinal health.
34959902	1	34	theme	diet	176:179	arg1	effects					163:169	the effects	159:169	the effects of a diet supplemented with fruits and vegetables (FV) on the host whole blood cell (WBC) transcriptome and the composition and function of the intestinal microbiome	159:335	A study was conducted to determine the effects of a diet supplemented with fruits and vegetables (FV) on the host whole blood cell (WBC) transcriptome and the composition and function of the intestinal microbiome.
34959902	7	35	theme	inflammatory	1484:1495	arg1	function					1508:1515	cellular inflammatory and immune function	1475:1515	cellular inflammatory and immune function in the WBC transcriptome	1475:1540	These results suggest that feeding an FV-supplemented diet for two weeks modulated markers of cellular inflammatory and immune function in the WBC transcriptome and the composition of the intestinal microbiome by increasing the abundance of bacterial taxa that have been associated with improved intestinal health.
34959902	1	36	theme	cell	250:253	arg1	transcriptome					261:273	the host whole blood cell (WBC) transcriptome	229:273	the host whole blood cell (WBC) transcriptome	229:273	A study was conducted to determine the effects of a diet supplemented with fruits and vegetables (FV) on the host whole blood cell (WBC) transcriptome and the composition and function of the intestinal microbiome.
34959902	2	37	theme	Dietary	507:513	arg1	Guideline					515:523	the Dietary Guideline	503:523	the Dietary Guideline for Americans (DGA) for two weeks	503:557	Nine six-week-old pigs were fed a pig grower diet alone or supplemented with lyophilized FV equivalent to half the daily recommended amount prescribed for humans by the Dietary Guideline for Americans (DGA) for two weeks.
34959902	2	38	theme	grower	376:381	arg1	diet					383:386	a pig grower diet	370:386	a pig grower diet alone	370:392	Nine six-week-old pigs were fed a pig grower diet alone or supplemented with lyophilized FV equivalent to half the daily recommended amount prescribed for humans by the Dietary Guideline for Americans (DGA) for two weeks.
34959902	5	39	theme	FV-supplemented	763:777	arg1	diet					779:782	an FV-supplemented diet	760:782	an FV-supplemented diet to pigs	760:790	Feeding an FV-supplemented diet to pigs induced differential expression of several genes associated with an increase in B-cell development and differentiation and the regulation of cellular movement, inflammatory response, and cell-to-cell signaling.
34959902	1	40	dep	composition	283:293	arg1	the					279:281	the	279:281	the	279:281	A study was conducted to determine the effects of a diet supplemented with fruits and vegetables (FV) on the host whole blood cell (WBC) transcriptome and the composition and function of the intestinal microbiome.
34959902	7	41	theme	taxa	1632:1635	arg1	abundance					1609:1617	the abundance	1605:1617	the abundance of bacterial taxa that have been associated with improved intestinal health	1605:1693	These results suggest that feeding an FV-supplemented diet for two weeks modulated markers of cellular inflammatory and immune function in the WBC transcriptome and the composition of the intestinal microbiome by increasing the abundance of bacterial taxa that have been associated with improved intestinal health.
34959902	6	42	from	Lachnospiraceae	1125:1139	arg1	genera					1113:1118	genera	1113:1118	genera from Lachnospiraceae and Ruminococcaceae families	1113:1168	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples showed differential increases in genera from Lachnospiraceae and Ruminococcaceae families within the order Clostridiales and Erysipelotrichaceae family with a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet.
34959902	6	42	from	Lachnospiraceae	1125:1139	arg1	increases					1100:1108	differential increases	1087:1108	differential increases in genera from Lachnospiraceae and Ruminococcaceae families	1087:1168	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples showed differential increases in genera from Lachnospiraceae and Ruminococcaceae families within the order Clostridiales and Erysipelotrichaceae family with a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet.
34959902	2	43	theme	pig	372:374	arg1	diet					383:386	a pig grower diet	370:386	a pig grower diet alone	370:392	Nine six-week-old pigs were fed a pig grower diet alone or supplemented with lyophilized FV equivalent to half the daily recommended amount prescribed for humans by the Dietary Guideline for Americans (DGA) for two weeks.
34959902	2	44	theme	equivalent	430:439	arg1	FV					427:428	lyophilized FV	415:428	lyophilized FV equivalent to half the daily recommended amount prescribed for humans by the Dietary Guideline for Americans (DGA) for two weeks	415:557	Nine six-week-old pigs were fed a pig grower diet alone or supplemented with lyophilized FV equivalent to half the daily recommended amount prescribed for humans by the Dietary Guideline for Americans (DGA) for two weeks.
34959902	6	45	theme	predicted	1239:1247	arg1	reduction					1249:1257	a predicted reduction	1237:1257	a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet	1237:1378	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples showed differential increases in genera from Lachnospiraceae and Ruminococcaceae families within the order Clostridiales and Erysipelotrichaceae family with a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet.
34959902	5	46	theme	several	827:833	arg1	genes					835:839	several genes	827:839	several genes associated with an increase in B-cell development and differentiation and the regulation of cellular movement, inflammatory response, and cell-to-cell signaling	827:1000	Feeding an FV-supplemented diet to pigs induced differential expression of several genes associated with an increase in B-cell development and differentiation and the regulation of cellular movement, inflammatory response, and cell-to-cell signaling.
34959902	6	47	theme	discriminant	1010:1021	arg1	analysis					1023:1030	Linear discriminant analysis	1003:1030	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples	1003:1078	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples showed differential increases in genera from Lachnospiraceae and Ruminococcaceae families within the order Clostridiales and Erysipelotrichaceae family with a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet.
34959902	7	48	from	transcriptome	1528:1540	arg1	composition					1550:1560	the composition	1546:1560	the composition of the intestinal microbiome	1546:1589	These results suggest that feeding an FV-supplemented diet for two weeks modulated markers of cellular inflammatory and immune function in the WBC transcriptome and the composition of the intestinal microbiome by increasing the abundance of bacterial taxa that have been associated with improved intestinal health.
34959902	7	48	from	transcriptome	1528:1540	arg1	markers					1464:1470	markers	1464:1470	markers of cellular inflammatory and immune function in the WBC transcriptome	1464:1540	These results suggest that feeding an FV-supplemented diet for two weeks modulated markers of cellular inflammatory and immune function in the WBC transcriptome and the composition of the intestinal microbiome by increasing the abundance of bacterial taxa that have been associated with improved intestinal health.
34959902	7	49	theme	intestinal	1677:1686	arg1	health					1688:1693	improved intestinal health	1668:1693	improved intestinal health	1668:1693	These results suggest that feeding an FV-supplemented diet for two weeks modulated markers of cellular inflammatory and immune function in the WBC transcriptome and the composition of the intestinal microbiome by increasing the abundance of bacterial taxa that have been associated with improved intestinal health.
34959902	6	50	theme	FV-supplemented	1359:1373	arg1	diet					1375:1378	the FV-supplemented diet	1355:1378	the FV-supplemented diet	1355:1378	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples showed differential increases in genera from Lachnospiraceae and Ruminococcaceae families within the order Clostridiales and Erysipelotrichaceae family with a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet.
34959902	7	51	theme	microbiome	1580:1589	arg1	composition					1550:1560	the composition	1546:1560	the composition of the intestinal microbiome	1546:1589	These results suggest that feeding an FV-supplemented diet for two weeks modulated markers of cellular inflammatory and immune function in the WBC transcriptome and the composition of the intestinal microbiome by increasing the abundance of bacterial taxa that have been associated with improved intestinal health.
34959902	7	51	theme	microbiome	1580:1589	arg1	markers					1464:1470	markers	1464:1470	markers of cellular inflammatory and immune function in the WBC transcriptome	1464:1540	These results suggest that feeding an FV-supplemented diet for two weeks modulated markers of cellular inflammatory and immune function in the WBC transcriptome and the composition of the intestinal microbiome by increasing the abundance of bacterial taxa that have been associated with improved intestinal health.
34959902	5	52	theme	cell-to-cell	979:990	arg1	signaling					992:1000	cell-to-cell signaling	979:1000	cell-to-cell signaling	979:1000	Feeding an FV-supplemented diet to pigs induced differential expression of several genes associated with an increase in B-cell development and differentiation and the regulation of cellular movement, inflammatory response, and cell-to-cell signaling.
34959902	2	53	theme	recommended	459:469	arg1	amount					471:476	the daily recommended amount	449:476	half the daily recommended amount prescribed for humans by the Dietary Guideline for Americans (DGA) for two weeks	444:557	Nine six-week-old pigs were fed a pig grower diet alone or supplemented with lyophilized FV equivalent to half the daily recommended amount prescribed for humans by the Dietary Guideline for Americans (DGA) for two weeks.
34959902	2	54	theme	lyophilized	415:425	arg1	FV					427:428	lyophilized FV	415:428	lyophilized FV equivalent to half the daily recommended amount prescribed for humans by the Dietary Guideline for Americans (DGA) for two weeks	415:557	Nine six-week-old pigs were fed a pig grower diet alone or supplemented with lyophilized FV equivalent to half the daily recommended amount prescribed for humans by the Dietary Guideline for Americans (DGA) for two weeks.
34959902	3	55	from	changes	579:585	arg1	WBC					594:596	the WBC	590:596	the WBC	590:596	Host transcriptome changes in the WBC were evaluated by RNA sequencing.
34959902	2	56	dep	half	444:447	arg1	amount					471:476	the daily recommended amount	449:476	half the daily recommended amount prescribed for humans by the Dietary Guideline for Americans (DGA) for two weeks	444:557	Nine six-week-old pigs were fed a pig grower diet alone or supplemented with lyophilized FV equivalent to half the daily recommended amount prescribed for humans by the Dietary Guideline for Americans (DGA) for two weeks.
34959902	5	57	theme	signaling	992:1000	arg1	regulation					919:928	the regulation	915:928	the regulation of cellular movement, inflammatory response, and cell-to-cell signaling	915:1000	Feeding an FV-supplemented diet to pigs induced differential expression of several genes associated with an increase in B-cell development and differentiation and the regulation of cellular movement, inflammatory response, and cell-to-cell signaling.
34959902	5	57	theme	signaling	992:1000	arg1	increase					860:867	an increase	857:867	an increase in B-cell development and differentiation	857:909	Feeding an FV-supplemented diet to pigs induced differential expression of several genes associated with an increase in B-cell development and differentiation and the regulation of cellular movement, inflammatory response, and cell-to-cell signaling.
34959902	7	58	theme	immune	1501:1506	arg1	function					1508:1515	cellular inflammatory and immune function	1475:1515	cellular inflammatory and immune function in the WBC transcriptome	1475:1540	These results suggest that feeding an FV-supplemented diet for two weeks modulated markers of cellular inflammatory and immune function in the WBC transcriptome and the composition of the intestinal microbiome by increasing the abundance of bacterial taxa that have been associated with improved intestinal health.
34959902	7	59	theme	FV-supplemented	1419:1433	arg1	diet					1435:1438	an FV-supplemented diet	1416:1438	an FV-supplemented diet for two weeks	1416:1452	These results suggest that feeding an FV-supplemented diet for two weeks modulated markers of cellular inflammatory and immune function in the WBC transcriptome and the composition of the intestinal microbiome by increasing the abundance of bacterial taxa that have been associated with improved intestinal health.
34959902	4	60	from	microbiome	660:669	arg1	DNA					641:643	Isolated DNA	632:643	Isolated DNA from the fecal microbiome	632:669	Isolated DNA from the fecal microbiome was used for 16S rDNA taxonomic analysis and prediction of metabolomic function.
34959902	6	61	theme	order	1181:1185	arg1	Clostridiales					1187:1199	the order Clostridiales and Erysipelotrichaceae family	1177:1230	Clostridiales	1187:1199	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples showed differential increases in genera from Lachnospiraceae and Ruminococcaceae families within the order Clostridiales and Erysipelotrichaceae family with a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet.
34959902	3	62	theme	RNA	616:618	arg1	sequencing					620:629	RNA sequencing	616:629	RNA sequencing	616:629	Host transcriptome changes in the WBC were evaluated by RNA sequencing.
34959902	3	63	theme	Host	560:563	arg1	changes					579:585	Host transcriptome changes	560:585	Host transcriptome changes in the WBC	560:596	Host transcriptome changes in the WBC were evaluated by RNA sequencing.
34959902	6	64	from	increases	1100:1108	arg1	Lachnospiraceae					1125:1139	Lachnospiraceae	1125:1139	Lachnospiraceae	1125:1139	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples showed differential increases in genera from Lachnospiraceae and Ruminococcaceae families within the order Clostridiales and Erysipelotrichaceae family with a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet.
34959902	6	64	from	increases	1100:1108	arg1	genera					1113:1118	genera	1113:1118	genera from Lachnospiraceae and Ruminococcaceae families	1113:1168	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples showed differential increases in genera from Lachnospiraceae and Ruminococcaceae families within the order Clostridiales and Erysipelotrichaceae family with a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet.
34959902	6	64	from	increases	1100:1108	arg1	Ruminococcaceae					1145:1159	Ruminococcaceae	1145:1159	Ruminococcaceae	1145:1159	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples showed differential increases in genera from Lachnospiraceae and Ruminococcaceae families within the order Clostridiales and Erysipelotrichaceae family with a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet.
34959902	5	65	theme	B-cell	872:877	arg1	development					879:889	B-cell development	872:889	B-cell development	872:889	Feeding an FV-supplemented diet to pigs induced differential expression of several genes associated with an increase in B-cell development and differentiation and the regulation of cellular movement, inflammatory response, and cell-to-cell signaling.
34959902	7	66	theme	cellular	1475:1482	arg1	function					1508:1515	cellular inflammatory and immune function	1475:1515	cellular inflammatory and immune function in the WBC transcriptome	1475:1540	These results suggest that feeding an FV-supplemented diet for two weeks modulated markers of cellular inflammatory and immune function in the WBC transcriptome and the composition of the intestinal microbiome by increasing the abundance of bacterial taxa that have been associated with improved intestinal health.
34959902	3	67	theme	transcriptome	565:577	arg1	changes					579:585	Host transcriptome changes	560:585	Host transcriptome changes in the WBC	560:596	Host transcriptome changes in the WBC were evaluated by RNA sequencing.
34959902	4	68	theme	fecal	654:658	arg1	microbiome					660:669	the fecal microbiome	650:669	the fecal microbiome	650:669	Isolated DNA from the fecal microbiome was used for 16S rDNA taxonomic analysis and prediction of metabolomic function.
34959902	6	69	theme	differential	1087:1098	arg1	increases					1100:1108	differential increases	1087:1108	differential increases in genera from Lachnospiraceae and Ruminococcaceae families	1087:1168	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples showed differential increases in genera from Lachnospiraceae and Ruminococcaceae families within the order Clostridiales and Erysipelotrichaceae family with a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet.
34959902	2	70	theme	daily	453:457	arg1	amount					471:476	the daily recommended amount	449:476	half the daily recommended amount prescribed for humans by the Dietary Guideline for Americans (DGA) for two weeks	444:557	Nine six-week-old pigs were fed a pig grower diet alone or supplemented with lyophilized FV equivalent to half the daily recommended amount prescribed for humans by the Dietary Guideline for Americans (DGA) for two weeks.
34959902	6	71	theme	microbiome	1061:1070	arg1	samples					1072:1078	fecal microbiome samples	1055:1078	fecal microbiome samples	1055:1078	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples showed differential increases in genera from Lachnospiraceae and Ruminococcaceae families within the order Clostridiales and Erysipelotrichaceae family with a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet.
34959902	4	72	theme	function	742:749	arg1	prediction					716:725	prediction	716:725	prediction of metabolomic function	716:749	Isolated DNA from the fecal microbiome was used for 16S rDNA taxonomic analysis and prediction of metabolomic function.
34959902	4	72	theme	function	742:749	arg1	analysis					703:710	rDNA taxonomic analysis	688:710	rDNA taxonomic analysis	688:710	Isolated DNA from the fecal microbiome was used for 16S rDNA taxonomic analysis and prediction of metabolomic function.
34959902	6	73	theme	effect	1032:1037	arg1	LEfSe					1045:1049	LEfSe	1045:1049	LEfSe	1045:1049	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples showed differential increases in genera from Lachnospiraceae and Ruminococcaceae families within the order Clostridiales and Erysipelotrichaceae family with a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet.
34959902	6	73	theme	effect	1032:1037	arg1	size					1039:1042	Linear discriminant analysis effect size	1003:1042	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples	1003:1078	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples showed differential increases in genera from Lachnospiraceae and Ruminococcaceae families within the order Clostridiales and Erysipelotrichaceae family with a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet.
34959902	5	74	theme	genes	835:839	arg1	expression					813:822	differential expression	800:822	differential expression of several genes associated with an increase in B-cell development and differentiation and the regulation of cellular movement, inflammatory response, and cell-to-cell signaling	800:1000	Feeding an FV-supplemented diet to pigs induced differential expression of several genes associated with an increase in B-cell development and differentiation and the regulation of cellular movement, inflammatory response, and cell-to-cell signaling.
34959902	7	75	from	markers	1464:1470	arg1	transcriptome					1528:1540	the WBC transcriptome	1520:1540	the WBC transcriptome	1520:1540	These results suggest that feeding an FV-supplemented diet for two weeks modulated markers of cellular inflammatory and immune function in the WBC transcriptome and the composition of the intestinal microbiome by increasing the abundance of bacterial taxa that have been associated with improved intestinal health.
34959902	6	76	from	biosynthesis	1330:1341	arg1	pigs					1346:1349	pigs	1346:1349	pigs fed the FV-supplemented diet	1346:1378	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples showed differential increases in genera from Lachnospiraceae and Ruminococcaceae families within the order Clostridiales and Erysipelotrichaceae family with a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet.
34959902	0	77	theme	Pig	52:54	arg1	Transcriptome					56:68	the Pig Transcriptome	48:68	the Pig Transcriptome	48:68	Fruit and Vegetable Supplemented Diet Modulates the Pig Transcriptome and Microbiome after a Two-Week Feeding Intervention.
34959902	4	78	theme	metabolomic	730:740	arg1	function					742:749	metabolomic function	730:749	metabolomic function	730:749	Isolated DNA from the fecal microbiome was used for 16S rDNA taxonomic analysis and prediction of metabolomic function.
34959902	1	79	from	effects	163:169	arg1	transcriptome					261:273	the host whole blood cell (WBC) transcriptome	229:273	the host whole blood cell (WBC) transcriptome	229:273	A study was conducted to determine the effects of a diet supplemented with fruits and vegetables (FV) on the host whole blood cell (WBC) transcriptome and the composition and function of the intestinal microbiome.
34959902	1	79	from	effects	163:169	arg1	composition					283:293	composition	283:293	composition	283:293	A study was conducted to determine the effects of a diet supplemented with fruits and vegetables (FV) on the host whole blood cell (WBC) transcriptome and the composition and function of the intestinal microbiome.
34959902	6	80	theme	Erysipelotrichaceae	1205:1223	arg1	family					1225:1230	the order Clostridiales and Erysipelotrichaceae family	1177:1230	family	1225:1230	Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples showed differential increases in genera from Lachnospiraceae and Ruminococcaceae families within the order Clostridiales and Erysipelotrichaceae family with a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet.
34959902	7	81	theme	intestinal	1569:1578	arg1	microbiome					1580:1589	the intestinal microbiome	1565:1589	the intestinal microbiome	1565:1589	These results suggest that feeding an FV-supplemented diet for two weeks modulated markers of cellular inflammatory and immune function in the WBC transcriptome and the composition of the intestinal microbiome by increasing the abundance of bacterial taxa that have been associated with improved intestinal health.
34431694	9	0	theme	communities	1669:1679	arg1	composition					1628:1638	composition	1628:1638	composition	1628:1638	IMPORTANCE Recent advances in microbial ecology have highlighted the importance of intercellular interactions in controlling the development, composition, and resilience of microbial communities.
34431694	9	0	theme	communities	1669:1679	arg1	resilience					1645:1654	resilience	1645:1654	resilience	1645:1654	IMPORTANCE Recent advances in microbial ecology have highlighted the importance of intercellular interactions in controlling the development, composition, and resilience of microbial communities.
34431694	9	0	theme	communities	1669:1679	arg1	development					1615:1625	development	1615:1625	development	1615:1625	IMPORTANCE Recent advances in microbial ecology have highlighted the importance of intercellular interactions in controlling the development, composition, and resilience of microbial communities.
34431694	11	1	theme	static	1905:1910	arg1	perturbations					1912:1924	static perturbations	1905:1924	static perturbations	1905:1924	Optogenetically controlled interactions offer advantages over static perturbations or chemically controlled interactions, as light can be manipulated in space and time and does not require the addition of nutrients or antibiotics.
34431694	8	2	from	control	1364:1370	arg1	cerevisiae					1414:1423	S. cerevisiae	1411:1423	S. cerevisiae	1411:1423	Our strategy can be applied to achieve spatiotemporal control of expression of a gene of interest in S. cerevisiae to perturb both intercellular and interspecies interactions.
34431694	9	3	theme	interactions	1583:1594	arg1	importance					1555:1564	the importance	1551:1564	the importance of intercellular interactions in controlling the development, composition, and resilience of microbial communities	1551:1679	IMPORTANCE Recent advances in microbial ecology have highlighted the importance of intercellular interactions in controlling the development, composition, and resilience of microbial communities.
34431694	7	4	theme	Spatial	1106:1112	arg1	control					1114:1120	Spatial control	1106:1120	Spatial control of light	1106:1129	Spatial control of light allows cooperators and cheaters to be spatially segregated, and we show that the interplay between cooperative and inhibitory interactions in space can lead to pattern formation.
34431694	2	5	theme	powerful	294:301	arg1	route					303:307	a powerful route	292:307	a powerful route to determining the critical interactions and design rules for microbial communities	292:391	The ability to perturb specific intercellular interactions in space and time provides a powerful route to determining the critical interactions and design rules for microbial communities.
34431694	9	6	theme	IMPORTANCE	1486:1495	arg1	advances					1504:1511	IMPORTANCE Recent advances	1486:1511	IMPORTANCE Recent advances in microbial ecology	1486:1532	IMPORTANCE Recent advances in microbial ecology have highlighted the importance of intercellular interactions in controlling the development, composition, and resilience of microbial communities.
34431694	12	7	theme	gene	2140:2143	arg1	control					2127:2133	light control	2121:2133	light control of a gene of interest	2121:2155	Here, we report a system for rapidly achieving light control of a gene of interest in the important model organism Saccharomyces cerevisiae and demonstrate that by controlling expression of the enzyme invertase, we can control cooperative interactions.
34431694	9	8	dep	development	1615:1625	arg1	the					1611:1613	the	1611:1613	the	1611:1613	IMPORTANCE Recent advances in microbial ecology have highlighted the importance of intercellular interactions in controlling the development, composition, and resilience of microbial communities.
34431694	8	9	from	cerevisiae	1414:1423	arg1	control					1364:1370	spatiotemporal control	1349:1370	spatiotemporal control of expression of a gene of interest in S. cerevisiae to perturb both intercellular and interspecies interactions	1349:1483	Our strategy can be applied to achieve spatiotemporal control of expression of a gene of interest in S. cerevisiae to perturb both intercellular and interspecies interactions.
34431694	13	10	theme	intercellular	2374:2386	arg1	interactions					2405:2416	intercellular and interspecies interactions	2374:2416	intercellular and interspecies interactions in natural and synthetic microbial consortia containing S. cerevisiae	2374:2486	This approach will be useful for understanding intercellular and interspecies interactions in natural and synthetic microbial consortia containing S. cerevisiae and serves as a proof of principle for implementing this approach in other consortia.
34431694	12	11	theme	light	2121:2125	arg1	control					2127:2133	light control	2121:2133	light control of a gene of interest	2121:2155	Here, we report a system for rapidly achieving light control of a gene of interest in the important model organism Saccharomyces cerevisiae and demonstrate that by controlling expression of the enzyme invertase, we can control cooperative interactions.
34431694	7	12	theme	cooperative	1230:1240	arg1	interactions					1257:1268	cooperative and inhibitory interactions	1230:1268	cooperative and inhibitory interactions in space	1230:1277	Spatial control of light allows cooperators and cheaters to be spatially segregated, and we show that the interplay between cooperative and inhibitory interactions in space can lead to pattern formation.
34431694	2	13	theme	intercellular	238:250	arg1	interactions					252:263	specific intercellular interactions	229:263	specific intercellular interactions	229:263	The ability to perturb specific intercellular interactions in space and time provides a powerful route to determining the critical interactions and design rules for microbial communities.
34431694	13	14	contain	containing	2463:2472	arg1	consortia					2453:2461	natural and synthetic microbial consortia	2421:2461	natural and synthetic microbial consortia containing S. cerevisiae	2421:2486	This approach will be useful for understanding intercellular and interspecies interactions in natural and synthetic microbial consortia containing S. cerevisiae and serves as a proof of principle for implementing this approach in other consortia.
34431694	13	14	contain	containing	2463:2472	arg2	cerevisiae					2477:2486	S. cerevisiae	2474:2486	S. cerevisiae	2474:2486	This approach will be useful for understanding intercellular and interspecies interactions in natural and synthetic microbial consortia containing S. cerevisiae and serves as a proof of principle for implementing this approach in other consortia.
34431694	5	15	theme	proof-of-principle	701:718	arg1	study					720:724	a proof-of-principle study	699:724	a proof-of-principle study	699:724	In a proof-of-principle study, we demonstrate the ability to control a model cooperative interaction, namely, the expression of the enzyme invertase (SUC2) which allows S. cerevisiae to hydrolyze sucrose and utilize it as a carbon source.
34431694	9	16	theme	Recent	1497:1502	arg1	advances					1504:1511	IMPORTANCE Recent advances	1486:1511	IMPORTANCE Recent advances in microbial ecology	1486:1532	IMPORTANCE Recent advances in microbial ecology have highlighted the importance of intercellular interactions in controlling the development, composition, and resilience of microbial communities.
34431694	5	17	theme	carbon	920:925	arg1	source					927:932	a carbon source	918:932	a carbon source	918:932	In a proof-of-principle study, we demonstrate the ability to control a model cooperative interaction, namely, the expression of the enzyme invertase (SUC2) which allows S. cerevisiae to hydrolyze sucrose and utilize it as a carbon source.
34431694	5	17	theme	carbon	920:925	arg1	it					912:913	it	912:913	it	912:913	In a proof-of-principle study, we demonstrate the ability to control a model cooperative interaction, namely, the expression of the enzyme invertase (SUC2) which allows S. cerevisiae to hydrolyze sucrose and utilize it as a carbon source.
34431694	5	18	theme	cooperative	773:783	arg1	interaction					785:795	a model cooperative interaction	765:795	a model cooperative interaction	765:795	In a proof-of-principle study, we demonstrate the ability to control a model cooperative interaction, namely, the expression of the enzyme invertase (SUC2) which allows S. cerevisiae to hydrolyze sucrose and utilize it as a carbon source.
34431694	10	19	theme	controlled	1824:1833	arg1	manner					1835:1840	a controlled manner	1822:1840	a controlled manner	1822:1840	In order to better understand the role of these interactions in governing community development, it is critical to be able to alter them in a controlled manner.
34431694	6	20	theme	interaction	988:998	arg1	strength					959:966	the strength	955:966	the strength of this cooperative interaction	955:998	We demonstrate that the strength of this cooperative interaction can be tuned in space and time by modulating light intensity and through spatial control of illumination.
34431694	0	21	from	Goods	40:44	arg1	cerevisiae					63:72	Saccharomyces cerevisiae	49:72	Saccharomyces cerevisiae	49:72	Optogenetic Tools for Control of Public Goods in Saccharomyces cerevisiae.
34431694	12	22	theme	enzyme	2268:2273	arg1	invertase					2275:2283	the enzyme invertase	2264:2283	the enzyme invertase	2264:2283	Here, we report a system for rapidly achieving light control of a gene of interest in the important model organism Saccharomyces cerevisiae and demonstrate that by controlling expression of the enzyme invertase, we can control cooperative interactions.
34431694	9	23	theme	intercellular	1569:1581	arg1	interactions					1583:1594	intercellular interactions	1569:1594	intercellular interactions	1569:1594	IMPORTANCE Recent advances in microbial ecology have highlighted the importance of intercellular interactions in controlling the development, composition, and resilience of microbial communities.
34431694	3	24	theme	optogenetic	411:421	arg1	tools					423:427	optogenetic tools	411:427	optogenetic tools	411:427	Approaches using optogenetic tools to modulate these interactions offer promise, as light can be exquisitely controlled in space and time.
34431694	0	25	theme	Optogenetic	0:10	arg1	Tools					12:16	Optogenetic Tools	0:16	Optogenetic Tools for Control of Public Goods in Saccharomyces cerevisiae	0:72	Optogenetic Tools for Control of Public Goods in Saccharomyces cerevisiae.
34431694	1	26	with	communities	116:126	arg1	interactions					134:145	interactions	134:145	interactions between cells guiding community development and phenotype	134:203	Microorganisms live in dense and diverse communities, with interactions between cells guiding community development and phenotype.
34431694	6	27	theme	light	1045:1049	arg1	intensity					1051:1059	light intensity	1045:1059	light intensity	1045:1059	We demonstrate that the strength of this cooperative interaction can be tuned in space and time by modulating light intensity and through spatial control of illumination.
34431694	8	28	theme	expression	1375:1384	arg1	control					1364:1370	spatiotemporal control	1349:1370	spatiotemporal control of expression of a gene of interest in S. cerevisiae to perturb both intercellular and interspecies interactions	1349:1483	Our strategy can be applied to achieve spatiotemporal control of expression of a gene of interest in S. cerevisiae to perturb both intercellular and interspecies interactions.
34431694	5	29	theme	invertase	835:843	arg1	expression					810:819	the expression	806:819	the expression of the enzyme invertase (SUC2) which allows S. cerevisiae to hydrolyze sucrose and utilize it as a carbon source	806:932	In a proof-of-principle study, we demonstrate the ability to control a model cooperative interaction, namely, the expression of the enzyme invertase (SUC2) which allows S. cerevisiae to hydrolyze sucrose and utilize it as a carbon source.
34431694	12	30	theme	Saccharomyces	2189:2201	arg1	organism					2180:2187	the important model organism	2160:2187	the important model organism Saccharomyces cerevisiae	2160:2212	Here, we report a system for rapidly achieving light control of a gene of interest in the important model organism Saccharomyces cerevisiae and demonstrate that by controlling expression of the enzyme invertase, we can control cooperative interactions.
34431694	4	31	theme	new	543:545	arg1	plasmids					547:554	new plasmids	543:554	new plasmids for rapid integration of an optogenetic system into Saccharomyces cerevisiae	543:631	We report new plasmids for rapid integration of an optogenetic system into Saccharomyces cerevisiae to engineer light control of expression of a gene of interest.
34431694	0	32	theme	Saccharomyces	49:61	arg1	cerevisiae					63:72	Saccharomyces cerevisiae	49:72	Saccharomyces cerevisiae	49:72	Optogenetic Tools for Control of Public Goods in Saccharomyces cerevisiae.
34431694	10	33	theme	interactions	1730:1741	arg1	role					1716:1719	the role	1712:1719	the role of these interactions in governing community development	1712:1776	In order to better understand the role of these interactions in governing community development, it is critical to be able to alter them in a controlled manner.
34431694	0	34	theme	Goods	40:44	arg1	Control					22:28	Control	22:28	Control of Public Goods in Saccharomyces cerevisiae	22:72	Optogenetic Tools for Control of Public Goods in Saccharomyces cerevisiae.
34431694	0	35	from	Control	22:28	arg1	cerevisiae					63:72	Saccharomyces cerevisiae	49:72	Saccharomyces cerevisiae	49:72	Optogenetic Tools for Control of Public Goods in Saccharomyces cerevisiae.
34431694	13	36	theme	microbial	2443:2451	arg1	consortia					2453:2461	natural and synthetic microbial consortia	2421:2461	natural and synthetic microbial consortia containing S. cerevisiae	2421:2486	This approach will be useful for understanding intercellular and interspecies interactions in natural and synthetic microbial consortia containing S. cerevisiae and serves as a proof of principle for implementing this approach in other consortia.
34431694	9	37	from	advances	1504:1511	arg1	ecology					1526:1532	microbial ecology	1516:1532	microbial ecology	1516:1532	IMPORTANCE Recent advances in microbial ecology have highlighted the importance of intercellular interactions in controlling the development, composition, and resilience of microbial communities.
34431694	11	38	theme	controlled	1859:1868	arg1	interactions					1870:1881	Optogenetically controlled interactions	1843:1881	Optogenetically controlled interactions	1843:1881	Optogenetically controlled interactions offer advantages over static perturbations or chemically controlled interactions, as light can be manipulated in space and time and does not require the addition of nutrients or antibiotics.
34431694	13	39	theme	interspecies	2392:2403	arg1	interactions					2405:2416	intercellular and interspecies interactions	2374:2416	intercellular and interspecies interactions in natural and synthetic microbial consortia containing S. cerevisiae	2374:2486	This approach will be useful for understanding intercellular and interspecies interactions in natural and synthetic microbial consortia containing S. cerevisiae and serves as a proof of principle for implementing this approach in other consortia.
34431694	12	40	theme	model	2174:2178	arg1	organism					2180:2187	the important model organism	2160:2187	the important model organism Saccharomyces cerevisiae	2160:2212	Here, we report a system for rapidly achieving light control of a gene of interest in the important model organism Saccharomyces cerevisiae and demonstrate that by controlling expression of the enzyme invertase, we can control cooperative interactions.
34431694	9	41	theme	microbial	1659:1667	arg1	communities					1669:1679	microbial communities	1659:1679	microbial communities	1659:1679	IMPORTANCE Recent advances in microbial ecology have highlighted the importance of intercellular interactions in controlling the development, composition, and resilience of microbial communities.
34431694	7	42	theme	inhibitory	1246:1255	arg1	interactions					1257:1268	cooperative and inhibitory interactions	1230:1268	cooperative and inhibitory interactions in space	1230:1277	Spatial control of light allows cooperators and cheaters to be spatially segregated, and we show that the interplay between cooperative and inhibitory interactions in space can lead to pattern formation.
34431694	4	43	theme	light	645:649	arg1	control					651:657	light control	645:657	light control of expression of a gene of interest	645:693	We report new plasmids for rapid integration of an optogenetic system into Saccharomyces cerevisiae to engineer light control of expression of a gene of interest.
34431694	2	44	theme	microbial	371:379	arg1	communities					381:391	microbial communities	371:391	microbial communities	371:391	The ability to perturb specific intercellular interactions in space and time provides a powerful route to determining the critical interactions and design rules for microbial communities.
34431694	6	45	theme	spatial	1073:1079	arg1	control					1081:1087	spatial control	1073:1087	spatial control of illumination	1073:1103	We demonstrate that the strength of this cooperative interaction can be tuned in space and time by modulating light intensity and through spatial control of illumination.
34431694	4	46	theme	system	596:601	arg1	integration					566:576	rapid integration	560:576	rapid integration of an optogenetic system into Saccharomyces cerevisiae	560:631	We report new plasmids for rapid integration of an optogenetic system into Saccharomyces cerevisiae to engineer light control of expression of a gene of interest.
34431694	2	47	theme	critical	328:335	arg1	interactions					337:348	the critical interactions	324:348	the critical interactions	324:348	The ability to perturb specific intercellular interactions in space and time provides a powerful route to determining the critical interactions and design rules for microbial communities.
34431694	13	48	theme	other	2557:2561	arg1	consortia					2563:2571	other consortia	2557:2571	other consortia	2557:2571	This approach will be useful for understanding intercellular and interspecies interactions in natural and synthetic microbial consortia containing S. cerevisiae and serves as a proof of principle for implementing this approach in other consortia.
34431694	12	49	theme	interest	2148:2155	arg1	gene					2140:2143	a gene	2138:2143	a gene of interest	2138:2155	Here, we report a system for rapidly achieving light control of a gene of interest in the important model organism Saccharomyces cerevisiae and demonstrate that by controlling expression of the enzyme invertase, we can control cooperative interactions.
34431694	1	50	theme	community	169:177	arg1	development					179:189	community development	169:189	community development	169:189	Microorganisms live in dense and diverse communities, with interactions between cells guiding community development and phenotype.
34431694	7	51	from	interactions	1257:1268	arg1	space					1273:1277	space	1273:1277	space	1273:1277	Spatial control of light allows cooperators and cheaters to be spatially segregated, and we show that the interplay between cooperative and inhibitory interactions in space can lead to pattern formation.
34431694	4	52	theme	gene	678:681	arg1	expression					662:671	expression	662:671	expression of a gene of interest	662:693	We report new plasmids for rapid integration of an optogenetic system into Saccharomyces cerevisiae to engineer light control of expression of a gene of interest.
34431694	11	53	theme	antibiotics	2061:2071	arg1	addition					2036:2043	the addition	2032:2043	the addition of nutrients or antibiotics	2032:2071	Optogenetically controlled interactions offer advantages over static perturbations or chemically controlled interactions, as light can be manipulated in space and time and does not require the addition of nutrients or antibiotics.
34431694	4	54	theme	interest	686:693	arg1	gene					678:681	a gene	676:681	a gene of interest	676:693	We report new plasmids for rapid integration of an optogenetic system into Saccharomyces cerevisiae to engineer light control of expression of a gene of interest.
34431694	8	55	theme	gene	1391:1394	arg1	expression					1375:1384	expression	1375:1384	expression of a gene of interest in S. cerevisiae	1375:1423	Our strategy can be applied to achieve spatiotemporal control of expression of a gene of interest in S. cerevisiae to perturb both intercellular and interspecies interactions.
34431694	11	56	theme	nutrients	2048:2056	arg1	addition					2036:2043	the addition	2032:2043	the addition of nutrients or antibiotics	2032:2071	Optogenetically controlled interactions offer advantages over static perturbations or chemically controlled interactions, as light can be manipulated in space and time and does not require the addition of nutrients or antibiotics.
34431694	2	57	theme	design	354:359	arg1	rules					361:365	design rules	354:365	design rules for microbial communities	354:391	The ability to perturb specific intercellular interactions in space and time provides a powerful route to determining the critical interactions and design rules for microbial communities.
34431694	12	58	dep	Saccharomyces	2189:2201	arg1	cerevisiae					2203:2212	cerevisiae	2203:2212	cerevisiae	2203:2212	Here, we report a system for rapidly achieving light control of a gene of interest in the important model organism Saccharomyces cerevisiae and demonstrate that by controlling expression of the enzyme invertase, we can control cooperative interactions.
34431694	8	59	theme	intercellular	1441:1453	arg1	interactions					1472:1483	both intercellular and interspecies interactions	1436:1483	both intercellular and interspecies interactions	1436:1483	Our strategy can be applied to achieve spatiotemporal control of expression of a gene of interest in S. cerevisiae to perturb both intercellular and interspecies interactions.
34431694	2	60	theme	specific	229:236	arg1	interactions					252:263	specific intercellular interactions	229:263	specific intercellular interactions	229:263	The ability to perturb specific intercellular interactions in space and time provides a powerful route to determining the critical interactions and design rules for microbial communities.
34431694	1	61	theme	diverse	108:114	arg1	communities					116:126	dense and diverse communities	98:126	dense and diverse communities	98:126	Microorganisms live in dense and diverse communities, with interactions between cells guiding community development and phenotype.
34431694	8	62	theme	interspecies	1459:1470	arg1	interactions					1472:1483	both intercellular and interspecies interactions	1436:1483	both intercellular and interspecies interactions	1436:1483	Our strategy can be applied to achieve spatiotemporal control of expression of a gene of interest in S. cerevisiae to perturb both intercellular and interspecies interactions.
34431694	13	63	theme	principle	2513:2521	arg1	proof					2504:2508	a proof	2502:2508	a proof of principle for implementing this approach in other consortia	2502:2571	This approach will be useful for understanding intercellular and interspecies interactions in natural and synthetic microbial consortia containing S. cerevisiae and serves as a proof of principle for implementing this approach in other consortia.
34431694	5	64	theme	model	767:771	arg1	interaction					785:795	a model cooperative interaction	765:795	a model cooperative interaction	765:795	In a proof-of-principle study, we demonstrate the ability to control a model cooperative interaction, namely, the expression of the enzyme invertase (SUC2) which allows S. cerevisiae to hydrolyze sucrose and utilize it as a carbon source.
34431694	4	65	theme	expression	662:671	arg1	control					651:657	light control	645:657	light control of expression of a gene of interest	645:693	We report new plasmids for rapid integration of an optogenetic system into Saccharomyces cerevisiae to engineer light control of expression of a gene of interest.
34431694	9	66	theme	microbial	1516:1524	arg1	ecology					1526:1532	microbial ecology	1516:1532	microbial ecology	1516:1532	IMPORTANCE Recent advances in microbial ecology have highlighted the importance of intercellular interactions in controlling the development, composition, and resilience of microbial communities.
34431694	12	67	theme	cooperative	2301:2311	arg1	interactions					2313:2324	cooperative interactions	2301:2324	cooperative interactions	2301:2324	Here, we report a system for rapidly achieving light control of a gene of interest in the important model organism Saccharomyces cerevisiae and demonstrate that by controlling expression of the enzyme invertase, we can control cooperative interactions.
34431694	6	68	theme	cooperative	976:986	arg1	interaction					988:998	this cooperative interaction	971:998	this cooperative interaction	971:998	We demonstrate that the strength of this cooperative interaction can be tuned in space and time by modulating light intensity and through spatial control of illumination.
34431694	13	69	from	interactions	2405:2416	arg1	consortia					2453:2461	natural and synthetic microbial consortia	2421:2461	natural and synthetic microbial consortia containing S. cerevisiae	2421:2486	This approach will be useful for understanding intercellular and interspecies interactions in natural and synthetic microbial consortia containing S. cerevisiae and serves as a proof of principle for implementing this approach in other consortia.
34431694	1	70	theme	live	90:93	arg1	Microorganisms					75:88	Microorganisms	75:88	Microorganisms live in dense and diverse communities, with interactions between cells guiding community development and phenotype	75:203	Microorganisms live in dense and diverse communities, with interactions between cells guiding community development and phenotype.
34431694	12	71	theme	invertase	2275:2283	arg1	expression					2250:2259	expression	2250:2259	expression of the enzyme invertase	2250:2283	Here, we report a system for rapidly achieving light control of a gene of interest in the important model organism Saccharomyces cerevisiae and demonstrate that by controlling expression of the enzyme invertase, we can control cooperative interactions.
34431694	8	72	theme	spatiotemporal	1349:1362	arg1	control					1364:1370	spatiotemporal control	1349:1370	spatiotemporal control of expression of a gene of interest in S. cerevisiae to perturb both intercellular and interspecies interactions	1349:1483	Our strategy can be applied to achieve spatiotemporal control of expression of a gene of interest in S. cerevisiae to perturb both intercellular and interspecies interactions.
34431694	11	73	theme	controlled	1940:1949	arg1	interactions					1951:1962	chemically controlled interactions	1929:1962	chemically controlled interactions	1929:1962	Optogenetically controlled interactions offer advantages over static perturbations or chemically controlled interactions, as light can be manipulated in space and time and does not require the addition of nutrients or antibiotics.
34431694	5	74	theme	enzyme	828:833	arg1	SUC2					846:849	SUC2	846:849	SUC2	846:849	In a proof-of-principle study, we demonstrate the ability to control a model cooperative interaction, namely, the expression of the enzyme invertase (SUC2) which allows S. cerevisiae to hydrolyze sucrose and utilize it as a carbon source.
34431694	5	74	theme	enzyme	828:833	arg1	invertase					835:843	the enzyme invertase	824:843	the enzyme invertase (SUC2) which allows S. cerevisiae to hydrolyze sucrose and utilize it as a carbon source	824:932	In a proof-of-principle study, we demonstrate the ability to control a model cooperative interaction, namely, the expression of the enzyme invertase (SUC2) which allows S. cerevisiae to hydrolyze sucrose and utilize it as a carbon source.
34431694	1	75	from	communities	116:126	arg1	live					90:93	live	90:93	live	90:93	Microorganisms live in dense and diverse communities, with interactions between cells guiding community development and phenotype.
34431694	1	76	theme	dense	98:102	arg1	communities					116:126	dense and diverse communities	98:126	dense and diverse communities	98:126	Microorganisms live in dense and diverse communities, with interactions between cells guiding community development and phenotype.
34431694	7	77	theme	pattern	1291:1297	arg1	formation					1299:1307	pattern formation	1291:1307	pattern formation	1291:1307	Spatial control of light allows cooperators and cheaters to be spatially segregated, and we show that the interplay between cooperative and inhibitory interactions in space can lead to pattern formation.
34431694	4	78	theme	optogenetic	584:594	arg1	system					596:601	an optogenetic system	581:601	an optogenetic system	581:601	We report new plasmids for rapid integration of an optogenetic system into Saccharomyces cerevisiae to engineer light control of expression of a gene of interest.
34431694	0	79	theme	Public	33:38	arg1	Goods					40:44	Public Goods	33:44	Public Goods in Saccharomyces cerevisiae	33:72	Optogenetic Tools for Control of Public Goods in Saccharomyces cerevisiae.
34431694	13	80	theme	natural	2421:2427	arg1	consortia					2453:2461	natural and synthetic microbial consortia	2421:2461	natural and synthetic microbial consortia containing S. cerevisiae	2421:2486	This approach will be useful for understanding intercellular and interspecies interactions in natural and synthetic microbial consortia containing S. cerevisiae and serves as a proof of principle for implementing this approach in other consortia.
34431694	7	81	theme	light	1125:1129	arg1	control					1114:1120	Spatial control	1106:1120	Spatial control of light	1106:1129	Spatial control of light allows cooperators and cheaters to be spatially segregated, and we show that the interplay between cooperative and inhibitory interactions in space can lead to pattern formation.
34431694	13	82	theme	synthetic	2433:2441	arg1	consortia					2453:2461	natural and synthetic microbial consortia	2421:2461	natural and synthetic microbial consortia containing S. cerevisiae	2421:2486	This approach will be useful for understanding intercellular and interspecies interactions in natural and synthetic microbial consortia containing S. cerevisiae and serves as a proof of principle for implementing this approach in other consortia.
34431694	4	83	theme	rapid	560:564	arg1	integration					566:576	rapid integration	560:576	rapid integration of an optogenetic system into Saccharomyces cerevisiae	560:631	We report new plasmids for rapid integration of an optogenetic system into Saccharomyces cerevisiae to engineer light control of expression of a gene of interest.
34431694	10	84	dep	understand	1701:1710	arg1	to					1691:1692	to	1691:1692	to	1691:1692	In order to better understand the role of these interactions in governing community development, it is critical to be able to alter them in a controlled manner.
34431694	0	85	from	cerevisiae	63:72	arg1	Control					22:28	Control	22:28	Control of Public Goods in Saccharomyces cerevisiae	22:72	Optogenetic Tools for Control of Public Goods in Saccharomyces cerevisiae.
34431694	8	86	theme	interest	1399:1406	arg1	gene					1391:1394	a gene	1389:1394	a gene of interest	1389:1406	Our strategy can be applied to achieve spatiotemporal control of expression of a gene of interest in S. cerevisiae to perturb both intercellular and interspecies interactions.
34431694	1	87	from	live	90:93	arg1	communities					116:126	dense and diverse communities	98:126	dense and diverse communities	98:126	Microorganisms live in dense and diverse communities, with interactions between cells guiding community development and phenotype.
34431694	6	88	theme	illumination	1092:1103	arg1	control					1081:1087	spatial control	1073:1087	spatial control of illumination	1073:1103	We demonstrate that the strength of this cooperative interaction can be tuned in space and time by modulating light intensity and through spatial control of illumination.
34431694	12	89	theme	important	2164:2172	arg1	organism					2180:2187	the important model organism	2160:2187	the important model organism Saccharomyces cerevisiae	2160:2212	Here, we report a system for rapidly achieving light control of a gene of interest in the important model organism Saccharomyces cerevisiae and demonstrate that by controlling expression of the enzyme invertase, we can control cooperative interactions.
34431694	10	90	theme	community	1756:1764	arg1	development					1766:1776	community development	1756:1776	community development	1756:1776	In order to better understand the role of these interactions in governing community development, it is critical to be able to alter them in a controlled manner.
34431694	8	91	from	expression	1375:1384	arg1	cerevisiae					1414:1423	S. cerevisiae	1411:1423	S. cerevisiae	1411:1423	Our strategy can be applied to achieve spatiotemporal control of expression of a gene of interest in S. cerevisiae to perturb both intercellular and interspecies interactions.
33242664	16	0	theme	peptidoglycan	2437:2449	arg1	recognition					2388:2398	recognition	2388:2398	recognition of bifidobacterial cell wall-derived peptidoglycan	2388:2449	These manifestations of DSS-induced dysbiosis were significantly attenuated by feeding B.a. Both the local and systemic ill-being of the mice alleviated when they received B.a. DISCUSSION This study shows that Pglyrp3 facilitates recognition of bifidobacterial cell wall-derived peptidoglycan, thus leading additively to a reduction of metaflammation through an increase in the number of bifidobacteria, which were able to mitigate intestinal immunopathology in the context of Pglyrp3 blockade.
33242664	10	1	theme	relevant	1473:1480	arg1	organs					1482:1487	relevant organs	1473:1487	relevant organs	1473:1487	Histology of relevant organs was carried out on day 8.
33242664	16	2	theme	cell	2419:2422	arg1	peptidoglycan					2437:2449	bifidobacterial cell wall-derived peptidoglycan	2403:2449	bifidobacterial cell wall-derived peptidoglycan	2403:2449	These manifestations of DSS-induced dysbiosis were significantly attenuated by feeding B.a. Both the local and systemic ill-being of the mice alleviated when they received B.a. DISCUSSION This study shows that Pglyrp3 facilitates recognition of bifidobacterial cell wall-derived peptidoglycan, thus leading additively to a reduction of metaflammation through an increase in the number of bifidobacteria, which were able to mitigate intestinal immunopathology in the context of Pglyrp3 blockade.
33242664	0	3	theme	wild-type	169:177	arg1	mice					184:187	Pglyrp3 knockout (Pglyrp3 -/-) and wild-type (WT) mice	134:187	Pglyrp3 knockout (Pglyrp3 -/-) and wild-type (WT) mice	134:187	Study on the additive protective effect of PGLYRP3 and Bifidobacterium adolescentis Reuter 1963 on severity of DSS-induced colitis in Pglyrp3 knockout (Pglyrp3 -/-) and wild-type (WT) mice.
33242664	16	4	theme	B.a.	2330:2333	arg1	DISCUSSION					2335:2344	B.a. DISCUSSION	2330:2344	B.a. DISCUSSION This study shows that Pglyrp3 facilitates recognition of bifidobacterial cell wall-derived peptidoglycan, thus leading additively to a reduction of metaflammation through an increase in the number of bifidobacteria, which were able to mitigate intestinal immunopathology in the context of Pglyrp3 blockade	2330:2650	These manifestations of DSS-induced dysbiosis were significantly attenuated by feeding B.a. Both the local and systemic ill-being of the mice alleviated when they received B.a. DISCUSSION This study shows that Pglyrp3 facilitates recognition of bifidobacterial cell wall-derived peptidoglycan, thus leading additively to a reduction of metaflammation through an increase in the number of bifidobacteria, which were able to mitigate intestinal immunopathology in the context of Pglyrp3 blockade.
33242664	1	5	theme	innate	236:241	arg1	Pglyrp3					210:216	Pglyrp3	210:216	Pglyrp3	210:216	BACKGROUND AND AIMS Pglyrp3 is a bactericidal innate immunity protein known to sustain the habitual gut microbiome and protect against experimental colitis.
33242664	1	5	theme	innate	236:241	arg1	protein					252:258	a bactericidal innate immunity protein	221:258	a bactericidal innate immunity protein known to sustain the habitual gut microbiome and protect against experimental colitis	221:344	BACKGROUND AND AIMS Pglyrp3 is a bactericidal innate immunity protein known to sustain the habitual gut microbiome and protect against experimental colitis.
33242664	6	6	theme	%	1129:1129	arg1	DSS					1131:1133	5% DSS	1128:1133	5% DSS	1128:1133	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	4	7	theme	mouse	711:715	arg1	models					717:722	DSS-induced mouse models	699:722	DSS-induced mouse models of intestinal inflammation	699:749	We investigated the extent to which Pglyrp3 and bifidobacteria regulate metaflammation and gut bacterial dysbiosis in DSS-induced mouse models of intestinal inflammation.
33242664	16	8	theme	DSS-induced	2182:2192	arg1	dysbiosis					2194:2202	DSS-induced dysbiosis	2182:2202	DSS-induced dysbiosis	2182:2202	These manifestations of DSS-induced dysbiosis were significantly attenuated by feeding B.a. Both the local and systemic ill-being of the mice alleviated when they received B.a. DISCUSSION This study shows that Pglyrp3 facilitates recognition of bifidobacterial cell wall-derived peptidoglycan, thus leading additively to a reduction of metaflammation through an increase in the number of bifidobacteria, which were able to mitigate intestinal immunopathology in the context of Pglyrp3 blockade.
33242664	14	9	theme	serum	1859:1863	arg1	cholesterol					1884:1894	cholesterol	1884:1894	cholesterol	1884:1894	Buildup of key serum metabolic markers (cholesterol, triglyceride and glucose) was set off by colonic inflammation.
33242664	14	9	theme	serum	1859:1863	arg1	markers					1875:1881	key serum metabolic markers	1855:1881	key serum metabolic markers (cholesterol, triglyceride and glucose)	1855:1921	Buildup of key serum metabolic markers (cholesterol, triglyceride and glucose) was set off by colonic inflammation.
33242664	14	9	theme	serum	1859:1863	arg1	glucose					1914:1920	glucose	1914:1920	glucose	1914:1920	Buildup of key serum metabolic markers (cholesterol, triglyceride and glucose) was set off by colonic inflammation.
33242664	14	9	theme	serum	1859:1863	arg1	triglyceride					1897:1908	triglyceride	1897:1908	triglyceride	1897:1908	Buildup of key serum metabolic markers (cholesterol, triglyceride and glucose) was set off by colonic inflammation.
33242664	6	10	theme	Pglyrp3	822:828	arg1	experiments					834:844	both WT and Pglyrp3 -/- experiments	810:844	experiments	834:844	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	9	11	theme	Faecal	1386:1391	arg1	samples					1400:1406	Faecal pellet samples	1386:1406	Faecal pellet samples	1386:1406	Faecal pellet samples were analysed by qRT-PCR for microbial composition.
33242664	16	12	theme	intestinal	2590:2599	arg1	immunopathology					2601:2615	intestinal immunopathology	2590:2615	intestinal immunopathology in the context of Pglyrp3 blockade	2590:2650	These manifestations of DSS-induced dysbiosis were significantly attenuated by feeding B.a. Both the local and systemic ill-being of the mice alleviated when they received B.a. DISCUSSION This study shows that Pglyrp3 facilitates recognition of bifidobacterial cell wall-derived peptidoglycan, thus leading additively to a reduction of metaflammation through an increase in the number of bifidobacteria, which were able to mitigate intestinal immunopathology in the context of Pglyrp3 blockade.
33242664	4	13	theme	bacterial	676:684	arg1	dysbiosis					686:694	gut bacterial dysbiosis	672:694	gut bacterial dysbiosis	672:694	We investigated the extent to which Pglyrp3 and bifidobacteria regulate metaflammation and gut bacterial dysbiosis in DSS-induced mouse models of intestinal inflammation.
33242664	0	14	theme	DSS-induced	111:121	arg1	colitis					123:129	DSS-induced colitis	111:129	DSS-induced colitis	111:129	Study on the additive protective effect of PGLYRP3 and Bifidobacterium adolescentis Reuter 1963 on severity of DSS-induced colitis in Pglyrp3 knockout (Pglyrp3 -/-) and wild-type (WT) mice.
33242664	16	15	theme	B.a.	2245:2248	arg1	ill-being					2278:2286	B.a. Both the local and systemic ill-being	2245:2286	B.a. Both the local and systemic ill-being of the mice alleviated when they received B.a. DISCUSSION This study shows that Pglyrp3 facilitates recognition of bifidobacterial cell wall-derived peptidoglycan, thus leading additively to a reduction of metaflammation through an increase in the number of bifidobacteria, which were able to mitigate intestinal immunopathology in the context of Pglyrp3 blockade	2245:2650	These manifestations of DSS-induced dysbiosis were significantly attenuated by feeding B.a. Both the local and systemic ill-being of the mice alleviated when they received B.a. DISCUSSION This study shows that Pglyrp3 facilitates recognition of bifidobacterial cell wall-derived peptidoglycan, thus leading additively to a reduction of metaflammation through an increase in the number of bifidobacteria, which were able to mitigate intestinal immunopathology in the context of Pglyrp3 blockade.
33242664	6	16	dep	group	932:936	arg1	a					922:922	a	922:922	a	922:922	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	6	16	dep	group	932:936	arg1	1					919:919	1	919:919	1	919:919	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	5	17	theme	old	782:784	arg1	mice					791:794	MATERIAL & METHODS 8-10 weeks old male mice	752:794	MATERIAL & METHODS 8-10 weeks old male mice	752:794	MATERIAL & METHODS 8-10 weeks old male mice were used.
33242664	14	18	dep	markers	1875:1881	arg1	cholesterol					1884:1894	cholesterol	1884:1894	cholesterol	1884:1894	Buildup of key serum metabolic markers (cholesterol, triglyceride and glucose) was set off by colonic inflammation.
33242664	14	18	dep	markers	1875:1881	arg1	markers					1875:1881	key serum metabolic markers	1855:1881	key serum metabolic markers (cholesterol, triglyceride and glucose)	1855:1921	Buildup of key serum metabolic markers (cholesterol, triglyceride and glucose) was set off by colonic inflammation.
33242664	14	18	dep	markers	1875:1881	arg1	glucose					1914:1920	glucose	1914:1920	glucose	1914:1920	Buildup of key serum metabolic markers (cholesterol, triglyceride and glucose) was set off by colonic inflammation.
33242664	14	18	dep	markers	1875:1881	arg1	triglyceride					1897:1908	triglyceride	1897:1908	triglyceride	1897:1908	Buildup of key serum metabolic markers (cholesterol, triglyceride and glucose) was set off by colonic inflammation.
33242664	15	19	theme	Bacteroides	2107:2117	arg1	group					2119:2123	Bacteroides group	2107:2123	Bacteroides group	2107:2123	qRT-PCR quantification showed that DSS significantly decreased the Clostridium coccoides and Bifidobacterium cell counts while increasing those of Bacteroides group in both WT and Pglyrp3 -/- mice.
33242664	6	20	theme	sterile	948:954	arg1	water					960:964	sterile tap water	948:964	sterile tap water	948:964	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	0	21	theme	knockout	142:149	arg1	mice					184:187	Pglyrp3 knockout (Pglyrp3 -/-) and wild-type (WT) mice	134:187	Pglyrp3 knockout (Pglyrp3 -/-) and wild-type (WT) mice	134:187	Study on the additive protective effect of PGLYRP3 and Bifidobacterium adolescentis Reuter 1963 on severity of DSS-induced colitis in Pglyrp3 knockout (Pglyrp3 -/-) and wild-type (WT) mice.
33242664	16	22	theme	Pglyrp3	2635:2641	arg1	blockade					2643:2650	Pglyrp3 blockade	2635:2650	Pglyrp3 blockade	2635:2650	These manifestations of DSS-induced dysbiosis were significantly attenuated by feeding B.a. Both the local and systemic ill-being of the mice alleviated when they received B.a. DISCUSSION This study shows that Pglyrp3 facilitates recognition of bifidobacterial cell wall-derived peptidoglycan, thus leading additively to a reduction of metaflammation through an increase in the number of bifidobacteria, which were able to mitigate intestinal immunopathology in the context of Pglyrp3 blockade.
33242664	0	23	from	severity	99:106	arg1	mice					184:187	Pglyrp3 knockout (Pglyrp3 -/-) and wild-type (WT) mice	134:187	Pglyrp3 knockout (Pglyrp3 -/-) and wild-type (WT) mice	134:187	Study on the additive protective effect of PGLYRP3 and Bifidobacterium adolescentis Reuter 1963 on severity of DSS-induced colitis in Pglyrp3 knockout (Pglyrp3 -/-) and wild-type (WT) mice.
33242664	16	24	theme	metaflammation	2494:2507	arg1	reduction					2481:2489	a reduction	2479:2489	a reduction of metaflammation through an increase in the number of bifidobacteria, which were able to mitigate intestinal immunopathology in the context of Pglyrp3 blockade	2479:2650	These manifestations of DSS-induced dysbiosis were significantly attenuated by feeding B.a. Both the local and systemic ill-being of the mice alleviated when they received B.a. DISCUSSION This study shows that Pglyrp3 facilitates recognition of bifidobacterial cell wall-derived peptidoglycan, thus leading additively to a reduction of metaflammation through an increase in the number of bifidobacteria, which were able to mitigate intestinal immunopathology in the context of Pglyrp3 blockade.
33242664	16	25	theme	local	2259:2263	arg1	ill-being					2278:2286	B.a. Both the local and systemic ill-being	2245:2286	B.a. Both the local and systemic ill-being of the mice alleviated when they received B.a. DISCUSSION This study shows that Pglyrp3 facilitates recognition of bifidobacterial cell wall-derived peptidoglycan, thus leading additively to a reduction of metaflammation through an increase in the number of bifidobacteria, which were able to mitigate intestinal immunopathology in the context of Pglyrp3 blockade	2245:2650	These manifestations of DSS-induced dysbiosis were significantly attenuated by feeding B.a. Both the local and systemic ill-being of the mice alleviated when they received B.a. DISCUSSION This study shows that Pglyrp3 facilitates recognition of bifidobacterial cell wall-derived peptidoglycan, thus leading additively to a reduction of metaflammation through an increase in the number of bifidobacteria, which were able to mitigate intestinal immunopathology in the context of Pglyrp3 blockade.
33242664	5	26	theme	MATERIAL	752:759	arg1	mice					791:794	MATERIAL & METHODS 8-10 weeks old male mice	752:794	MATERIAL & METHODS 8-10 weeks old male mice	752:794	MATERIAL & METHODS 8-10 weeks old male mice were used.
33242664	9	27	theme	microbial	1437:1445	arg1	composition					1447:1457	microbial composition	1437:1457	microbial composition	1437:1457	Faecal pellet samples were analysed by qRT-PCR for microbial composition.
33242664	6	28	theme	tap	1011:1013	arg1	water					1015:1019	sterile tap water	1003:1019	sterile tap water supplemented with only 5% DSS	1003:1049	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	4	29	theme	inflammation	738:749	arg1	models					717:722	DSS-induced mouse models	699:722	DSS-induced mouse models of intestinal inflammation	699:749	We investigated the extent to which Pglyrp3 and bifidobacteria regulate metaflammation and gut bacterial dysbiosis in DSS-induced mouse models of intestinal inflammation.
33242664	16	30	theme	systemic	2269:2276	arg1	ill-being					2278:2286	B.a. Both the local and systemic ill-being	2245:2286	B.a. Both the local and systemic ill-being of the mice alleviated when they received B.a. DISCUSSION This study shows that Pglyrp3 facilitates recognition of bifidobacterial cell wall-derived peptidoglycan, thus leading additively to a reduction of metaflammation through an increase in the number of bifidobacteria, which were able to mitigate intestinal immunopathology in the context of Pglyrp3 blockade	2245:2650	These manifestations of DSS-induced dysbiosis were significantly attenuated by feeding B.a. Both the local and systemic ill-being of the mice alleviated when they received B.a. DISCUSSION This study shows that Pglyrp3 facilitates recognition of bifidobacterial cell wall-derived peptidoglycan, thus leading additively to a reduction of metaflammation through an increase in the number of bifidobacteria, which were able to mitigate intestinal immunopathology in the context of Pglyrp3 blockade.
33242664	0	31	theme	adolescentis	71:82	arg1	effect					33:38	the additive protective effect	9:38	the additive protective effect of PGLYRP3 and Bifidobacterium adolescentis Reuter 1963 on severity of DSS-induced colitis in Pglyrp3 knockout (Pglyrp3 -/-) and wild-type (WT) mice	9:187	Study on the additive protective effect of PGLYRP3 and Bifidobacterium adolescentis Reuter 1963 on severity of DSS-induced colitis in Pglyrp3 knockout (Pglyrp3 -/-) and wild-type (WT) mice.
33242664	6	32	theme	experimental	974:985	arg1	group					987:991	an experimental group	971:991	(2) an experimental group receiving sterile tap water supplemented with only 5% DSS	967:1049	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	0	33	from	Study	0:4	arg1	effect					33:38	the additive protective effect	9:38	the additive protective effect of PGLYRP3 and Bifidobacterium adolescentis Reuter 1963 on severity of DSS-induced colitis in Pglyrp3 knockout (Pglyrp3 -/-) and wild-type (WT) mice	9:187	Study on the additive protective effect of PGLYRP3 and Bifidobacterium adolescentis Reuter 1963 on severity of DSS-induced colitis in Pglyrp3 knockout (Pglyrp3 -/-) and wild-type (WT) mice.
33242664	16	34	theme	mice	2295:2298	arg1	ill-being					2278:2286	B.a. Both the local and systemic ill-being	2245:2286	B.a. Both the local and systemic ill-being of the mice alleviated when they received B.a. DISCUSSION This study shows that Pglyrp3 facilitates recognition of bifidobacterial cell wall-derived peptidoglycan, thus leading additively to a reduction of metaflammation through an increase in the number of bifidobacteria, which were able to mitigate intestinal immunopathology in the context of Pglyrp3 blockade	2245:2650	These manifestations of DSS-induced dysbiosis were significantly attenuated by feeding B.a. Both the local and systemic ill-being of the mice alleviated when they received B.a. DISCUSSION This study shows that Pglyrp3 facilitates recognition of bifidobacterial cell wall-derived peptidoglycan, thus leading additively to a reduction of metaflammation through an increase in the number of bifidobacteria, which were able to mitigate intestinal immunopathology in the context of Pglyrp3 blockade.
33242664	1	35	theme	experimental	325:336	arg1	colitis					338:344	experimental colitis	325:344	experimental colitis	325:344	BACKGROUND AND AIMS Pglyrp3 is a bactericidal innate immunity protein known to sustain the habitual gut microbiome and protect against experimental colitis.
33242664	15	36	theme	Clostridium	2027:2037	arg1	coccoides					2039:2047	the Clostridium coccoides	2023:2047	the Clostridium coccoides	2023:2047	qRT-PCR quantification showed that DSS significantly decreased the Clostridium coccoides and Bifidobacterium cell counts while increasing those of Bacteroides group in both WT and Pglyrp3 -/- mice.
33242664	12	37	theme	Intestinal	1601:1610	arg1	inflammation					1612:1623	Intestinal inflammation	1601:1623	Intestinal inflammation in mice of group 2	1601:1642	RESULTS Intestinal inflammation in mice of group 2 were significantly increased compared to those of control group 1.
33242664	0	38	dep	knockout	142:149	arg1	WT					180:181	WT	180:181	WT	180:181	Study on the additive protective effect of PGLYRP3 and Bifidobacterium adolescentis Reuter 1963 on severity of DSS-induced colitis in Pglyrp3 knockout (Pglyrp3 -/-) and wild-type (WT) mice.
33242664	0	38	dep	knockout	142:149	arg1	-/-					160:162	-/-	160:162	-/-	160:162	Study on the additive protective effect of PGLYRP3 and Bifidobacterium adolescentis Reuter 1963 on severity of DSS-induced colitis in Pglyrp3 knockout (Pglyrp3 -/-) and wild-type (WT) mice.
33242664	16	39	from	increase	2520:2527	arg1	number					2536:2541	the number	2532:2541	the number of bifidobacteria, which were able to mitigate intestinal immunopathology in the context of Pglyrp3 blockade	2532:2650	These manifestations of DSS-induced dysbiosis were significantly attenuated by feeding B.a. Both the local and systemic ill-being of the mice alleviated when they received B.a. DISCUSSION This study shows that Pglyrp3 facilitates recognition of bifidobacterial cell wall-derived peptidoglycan, thus leading additively to a reduction of metaflammation through an increase in the number of bifidobacteria, which were able to mitigate intestinal immunopathology in the context of Pglyrp3 blockade.
33242664	14	40	theme	colonic	1938:1944	arg1	inflammation					1946:1957	colonic inflammation	1938:1957	colonic inflammation	1938:1957	Buildup of key serum metabolic markers (cholesterol, triglyceride and glucose) was set off by colonic inflammation.
33242664	16	41	theme	dysbiosis	2194:2202	arg1	manifestations					2164:2177	These manifestations	2158:2177	These manifestations of DSS-induced dysbiosis	2158:2202	These manifestations of DSS-induced dysbiosis were significantly attenuated by feeding B.a. Both the local and systemic ill-being of the mice alleviated when they received B.a. DISCUSSION This study shows that Pglyrp3 facilitates recognition of bifidobacterial cell wall-derived peptidoglycan, thus leading additively to a reduction of metaflammation through an increase in the number of bifidobacteria, which were able to mitigate intestinal immunopathology in the context of Pglyrp3 blockade.
33242664	2	42	theme	commensal	441:449	arg1	bifidobacteria					451:464	commensal bifidobacteria	441:464	commensal bifidobacteria	441:464	Intestinal inflammation and metaflammation are commonly associated with a marked reduction of commensal bifidobacteria.
33242664	13	43	theme	Pglyrp3	1826:1832	arg1	mice					1838:1841	DSS-treated WT and DSS-treated Pglyrp3 -/- mice	1795:1841	DSS-treated WT and DSS-treated Pglyrp3 -/- mice	1795:1841	There was a significant difference in mean scores for inflammation severity between DSS-treated WT and DSS-treated Pglyrp3 -/- mice.
33242664	1	44	theme	immunity	243:250	arg1	Pglyrp3					210:216	Pglyrp3	210:216	Pglyrp3	210:216	BACKGROUND AND AIMS Pglyrp3 is a bactericidal innate immunity protein known to sustain the habitual gut microbiome and protect against experimental colitis.
33242664	1	44	theme	immunity	243:250	arg1	protein					252:258	a bactericidal innate immunity protein	221:258	a bactericidal innate immunity protein known to sustain the habitual gut microbiome and protect against experimental colitis	221:344	BACKGROUND AND AIMS Pglyrp3 is a bactericidal innate immunity protein known to sustain the habitual gut microbiome and protect against experimental colitis.
33242664	6	45	theme	%	1045:1045	arg1	DSS					1047:1049	only 5% DSS	1039:1049	only 5% DSS	1039:1049	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	6	46	theme	mice	902:905	arg1	group					932:936	control group	924:936	control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS	924:1133	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	6	46	theme	mice	902:905	arg1	adolescentis					1173:1184	Bifidobacterium adolescentis	1157:1184	Bifidobacterium adolescentis (B.a.)	1157:1191	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	6	46	theme	mice	902:905	arg1	CFU/ml					1147:1152	1 × 109 CFU/ml	1139:1152	1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.)	1139:1191	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	6	46	theme	mice	902:905	arg1	groups					889:894	three groups	883:894	three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days	883:1202	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	6	46	theme	mice	902:905	arg1	mice					902:905	16 mice	899:905	16 mice	899:905	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	7	47	dep	Wild-type	1205:1213	arg1	WT					1216:1217	WT	1216:1217	WT	1216:1217	Wild-type (WT) littermates of the respective gene (i.e. Pglyrp3) were used as controls throughout the study.
33242664	7	48	theme	gene	1250:1253	arg1	controls					1283:1290	controls	1283:1290	controls	1283:1290	Wild-type (WT) littermates of the respective gene (i.e. Pglyrp3) were used as controls throughout the study.
33242664	7	48	theme	gene	1250:1253	arg1	littermates					1220:1230	Wild-type (WT) littermates	1205:1230	Wild-type (WT) littermates of the respective gene (i.e. Pglyrp3)	1205:1268	Wild-type (WT) littermates of the respective gene (i.e. Pglyrp3) were used as controls throughout the study.
33242664	0	49	theme	protective	22:31	arg1	effect					33:38	the additive protective effect	9:38	the additive protective effect of PGLYRP3 and Bifidobacterium adolescentis Reuter 1963 on severity of DSS-induced colitis in Pglyrp3 knockout (Pglyrp3 -/-) and wild-type (WT) mice	9:187	Study on the additive protective effect of PGLYRP3 and Bifidobacterium adolescentis Reuter 1963 on severity of DSS-induced colitis in Pglyrp3 knockout (Pglyrp3 -/-) and wild-type (WT) mice.
33242664	6	50	dep	groups	889:894	arg1	adolescentis					1173:1184	Bifidobacterium adolescentis	1157:1184	Bifidobacterium adolescentis (B.a.)	1157:1191	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	6	50	dep	groups	889:894	arg1	group					932:936	control group	924:936	control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS	924:1133	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	6	50	dep	groups	889:894	arg1	B.a.					1187:1190	B.a.	1187:1190	B.a.	1187:1190	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	6	50	dep	groups	889:894	arg1	groups					889:894	three groups	883:894	three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days	883:1202	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	6	50	dep	groups	889:894	arg1	CFU/ml					1147:1152	1 × 109 CFU/ml	1139:1152	1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.)	1139:1191	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	6	50	dep	groups	889:894	arg1	mice					902:905	16 mice	899:905	16 mice	899:905	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	8	51	theme	general	1332:1338	arg1	health					1340:1345	general health	1332:1345	general health	1332:1345	Clinical signs of general health and inflammation were monitored daily.
33242664	1	52	theme	habitual	281:288	arg1	microbiome					294:303	the habitual gut microbiome	277:303	the habitual gut microbiome	277:303	BACKGROUND AND AIMS Pglyrp3 is a bactericidal innate immunity protein known to sustain the habitual gut microbiome and protect against experimental colitis.
33242664	6	53	dep	group	1076:1080	arg1	3					1057:1057	3	1057:1057	3	1057:1057	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	11	54	theme	Metabolic	1515:1523	arg1	parameters					1525:1534	Metabolic parameters	1515:1534	Metabolic parameters	1515:1534	Metabolic parameters and liver inflammation were determined in serum samples.
33242664	15	55	theme	cell	2069:2072	arg1	counts					2074:2079	Bifidobacterium cell counts	2053:2079	Bifidobacterium cell counts	2053:2079	qRT-PCR quantification showed that DSS significantly decreased the Clostridium coccoides and Bifidobacterium cell counts while increasing those of Bacteroides group in both WT and Pglyrp3 -/- mice.
33242664	6	56	theme	experimental	1063:1074	arg1	group					1076:1080	an experimental group	1060:1080	(3) an experimental group receiving sterile tap water supplemented with 5% DSS	1056:1133	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	2	57	theme	Intestinal	347:356	arg1	inflammation					358:369	inflammation	358:369	inflammation	358:369	Intestinal inflammation and metaflammation are commonly associated with a marked reduction of commensal bifidobacteria.
33242664	15	58	theme	Pglyrp3	2140:2146	arg1	mice					2152:2155	both WT and Pglyrp3 -/- mice	2128:2155	mice	2152:2155	qRT-PCR quantification showed that DSS significantly decreased the Clostridium coccoides and Bifidobacterium cell counts while increasing those of Bacteroides group in both WT and Pglyrp3 -/- mice.
33242664	6	59	theme	control	924:930	arg1	group					932:936	control group	924:936	control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS	924:1133	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	6	59	theme	control	924:930	arg1	B.a.					1187:1190	B.a.	1187:1190	B.a.	1187:1190	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	6	59	theme	control	924:930	arg1	groups					889:894	three groups	883:894	three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days	883:1202	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	6	59	theme	control	924:930	arg1	adolescentis					1173:1184	Bifidobacterium adolescentis	1157:1184	Bifidobacterium adolescentis (B.a.)	1157:1191	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	6	59	theme	control	924:930	arg1	mice					902:905	16 mice	899:905	16 mice	899:905	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	14	60	theme	markers	1875:1881	arg1	Buildup					1844:1850	Buildup	1844:1850	Buildup of key serum metabolic markers (cholesterol, triglyceride and glucose)	1844:1921	Buildup of key serum metabolic markers (cholesterol, triglyceride and glucose) was set off by colonic inflammation.
33242664	7	61	theme	Wild-type	1205:1213	arg1	controls					1283:1290	controls	1283:1290	controls	1283:1290	Wild-type (WT) littermates of the respective gene (i.e. Pglyrp3) were used as controls throughout the study.
33242664	7	61	theme	Wild-type	1205:1213	arg1	littermates					1220:1230	Wild-type (WT) littermates	1205:1230	Wild-type (WT) littermates of the respective gene (i.e. Pglyrp3)	1205:1268	Wild-type (WT) littermates of the respective gene (i.e. Pglyrp3) were used as controls throughout the study.
33242664	10	62	theme	organs	1482:1487	arg1	Histology					1460:1468	Histology	1460:1468	Histology of relevant organs	1460:1487	Histology of relevant organs was carried out on day 8.
33242664	12	63	dep	RESULTS	1593:1599	arg1	increased					1663:1671	increased	1663:1671	were significantly increased compared to those of control group 1	1644:1708	RESULTS Intestinal inflammation in mice of group 2 were significantly increased compared to those of control group 1.
33242664	16	64	link	wall-derived	2424:2435	arg1	peptidoglycan					2437:2449	bifidobacterial cell wall-derived peptidoglycan	2403:2449	bifidobacterial cell wall-derived peptidoglycan	2403:2449	These manifestations of DSS-induced dysbiosis were significantly attenuated by feeding B.a. Both the local and systemic ill-being of the mice alleviated when they received B.a. DISCUSSION This study shows that Pglyrp3 facilitates recognition of bifidobacterial cell wall-derived peptidoglycan, thus leading additively to a reduction of metaflammation through an increase in the number of bifidobacteria, which were able to mitigate intestinal immunopathology in the context of Pglyrp3 blockade.
33242664	11	65	theme	serum	1578:1582	arg1	samples					1584:1590	serum samples	1578:1590	serum samples	1578:1590	Metabolic parameters and liver inflammation were determined in serum samples.
33242664	16	66	from	immunopathology	2601:2615	arg1	context					2624:2630	the context	2620:2630	the context of Pglyrp3 blockade	2620:2650	These manifestations of DSS-induced dysbiosis were significantly attenuated by feeding B.a. Both the local and systemic ill-being of the mice alleviated when they received B.a. DISCUSSION This study shows that Pglyrp3 facilitates recognition of bifidobacterial cell wall-derived peptidoglycan, thus leading additively to a reduction of metaflammation through an increase in the number of bifidobacteria, which were able to mitigate intestinal immunopathology in the context of Pglyrp3 blockade.
33242664	1	67	theme	bactericidal	223:234	arg1	Pglyrp3					210:216	Pglyrp3	210:216	Pglyrp3	210:216	BACKGROUND AND AIMS Pglyrp3 is a bactericidal innate immunity protein known to sustain the habitual gut microbiome and protect against experimental colitis.
33242664	1	67	theme	bactericidal	223:234	arg1	protein					252:258	a bactericidal innate immunity protein	221:258	a bactericidal innate immunity protein known to sustain the habitual gut microbiome and protect against experimental colitis	221:344	BACKGROUND AND AIMS Pglyrp3 is a bactericidal innate immunity protein known to sustain the habitual gut microbiome and protect against experimental colitis.
33242664	12	68	from	inflammation	1612:1623	arg1	mice					1628:1631	mice	1628:1631	mice of group 2	1628:1642	RESULTS Intestinal inflammation in mice of group 2 were significantly increased compared to those of control group 1.
33242664	13	69	theme	significant	1723:1733	arg1	difference					1735:1744	a significant difference	1721:1744	a significant difference in mean scores for inflammation severity between DSS-treated WT and DSS-treated Pglyrp3 -/- mice	1721:1841	There was a significant difference in mean scores for inflammation severity between DSS-treated WT and DSS-treated Pglyrp3 -/- mice.
33242664	14	70	theme	metabolic	1865:1873	arg1	cholesterol					1884:1894	cholesterol	1884:1894	cholesterol	1884:1894	Buildup of key serum metabolic markers (cholesterol, triglyceride and glucose) was set off by colonic inflammation.
33242664	14	70	theme	metabolic	1865:1873	arg1	markers					1875:1881	key serum metabolic markers	1855:1881	key serum metabolic markers (cholesterol, triglyceride and glucose)	1855:1921	Buildup of key serum metabolic markers (cholesterol, triglyceride and glucose) was set off by colonic inflammation.
33242664	14	70	theme	metabolic	1865:1873	arg1	glucose					1914:1920	glucose	1914:1920	glucose	1914:1920	Buildup of key serum metabolic markers (cholesterol, triglyceride and glucose) was set off by colonic inflammation.
33242664	14	70	theme	metabolic	1865:1873	arg1	triglyceride					1897:1908	triglyceride	1897:1908	triglyceride	1897:1908	Buildup of key serum metabolic markers (cholesterol, triglyceride and glucose) was set off by colonic inflammation.
33242664	4	71	theme	DSS-induced	699:709	arg1	models					717:722	DSS-induced mouse models	699:722	DSS-induced mouse models of intestinal inflammation	699:749	We investigated the extent to which Pglyrp3 and bifidobacteria regulate metaflammation and gut bacterial dysbiosis in DSS-induced mouse models of intestinal inflammation.
33242664	6	72	theme	-/-	830:832	arg1	experiments					834:844	both WT and Pglyrp3 -/- experiments	810:844	experiments	834:844	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	14	73	theme	key	1855:1857	arg1	cholesterol					1884:1894	cholesterol	1884:1894	cholesterol	1884:1894	Buildup of key serum metabolic markers (cholesterol, triglyceride and glucose) was set off by colonic inflammation.
33242664	14	73	theme	key	1855:1857	arg1	markers					1875:1881	key serum metabolic markers	1855:1881	key serum metabolic markers (cholesterol, triglyceride and glucose)	1855:1921	Buildup of key serum metabolic markers (cholesterol, triglyceride and glucose) was set off by colonic inflammation.
33242664	14	73	theme	key	1855:1857	arg1	glucose					1914:1920	glucose	1914:1920	glucose	1914:1920	Buildup of key serum metabolic markers (cholesterol, triglyceride and glucose) was set off by colonic inflammation.
33242664	14	73	theme	key	1855:1857	arg1	triglyceride					1897:1908	triglyceride	1897:1908	triglyceride	1897:1908	Buildup of key serum metabolic markers (cholesterol, triglyceride and glucose) was set off by colonic inflammation.
33242664	12	74	theme	control	1694:1700	arg1	group					1702:1706	control group 1	1694:1708	control group 1	1694:1708	RESULTS Intestinal inflammation in mice of group 2 were significantly increased compared to those of control group 1.
33242664	0	75	theme	colitis	123:129	arg1	severity					99:106	severity	99:106	severity of DSS-induced colitis in Pglyrp3 knockout (Pglyrp3 -/-) and wild-type (WT) mice	99:187	Study on the additive protective effect of PGLYRP3 and Bifidobacterium adolescentis Reuter 1963 on severity of DSS-induced colitis in Pglyrp3 knockout (Pglyrp3 -/-) and wild-type (WT) mice.
33242664	9	76	theme	pellet	1393:1398	arg1	samples					1400:1406	Faecal pellet samples	1386:1406	Faecal pellet samples	1386:1406	Faecal pellet samples were analysed by qRT-PCR for microbial composition.
33242664	6	77	theme	tap	1100:1102	arg1	water					1104:1108	sterile tap water	1092:1108	sterile tap water supplemented with 5% DSS	1092:1133	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	4	78	theme	gut	672:674	arg1	dysbiosis					686:694	gut bacterial dysbiosis	672:694	gut bacterial dysbiosis	672:694	We investigated the extent to which Pglyrp3 and bifidobacteria regulate metaflammation and gut bacterial dysbiosis in DSS-induced mouse models of intestinal inflammation.
33242664	5	79	theme	8-10 weeks	771:780	arg1	mice					791:794	MATERIAL & METHODS 8-10 weeks old male mice	752:794	MATERIAL & METHODS 8-10 weeks old male mice	752:794	MATERIAL & METHODS 8-10 weeks old male mice were used.
33242664	13	80	from	difference	1735:1744	arg1	scores					1754:1759	mean scores	1749:1759	mean scores for inflammation severity	1749:1785	There was a significant difference in mean scores for inflammation severity between DSS-treated WT and DSS-treated Pglyrp3 -/- mice.
33242664	6	81	theme	tap	956:958	arg1	water					960:964	sterile tap water	948:964	sterile tap water	948:964	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	1	82	dep	BACKGROUND	190:199	arg1	Pglyrp3					210:216	Pglyrp3	210:216	Pglyrp3	210:216	BACKGROUND AND AIMS Pglyrp3 is a bactericidal innate immunity protein known to sustain the habitual gut microbiome and protect against experimental colitis.
33242664	1	82	dep	BACKGROUND	190:199	arg1	protein					252:258	a bactericidal innate immunity protein	221:258	a bactericidal innate immunity protein known to sustain the habitual gut microbiome and protect against experimental colitis	221:344	BACKGROUND AND AIMS Pglyrp3 is a bactericidal innate immunity protein known to sustain the habitual gut microbiome and protect against experimental colitis.
33242664	16	83	theme	blockade	2643:2650	arg1	context					2624:2630	the context	2620:2630	the context of Pglyrp3 blockade	2620:2650	These manifestations of DSS-induced dysbiosis were significantly attenuated by feeding B.a. Both the local and systemic ill-being of the mice alleviated when they received B.a. DISCUSSION This study shows that Pglyrp3 facilitates recognition of bifidobacterial cell wall-derived peptidoglycan, thus leading additively to a reduction of metaflammation through an increase in the number of bifidobacteria, which were able to mitigate intestinal immunopathology in the context of Pglyrp3 blockade.
33242664	5	84	theme	&	761:761	arg1	mice					791:794	MATERIAL & METHODS 8-10 weeks old male mice	752:794	MATERIAL & METHODS 8-10 weeks old male mice	752:794	MATERIAL & METHODS 8-10 weeks old male mice were used.
33242664	6	85	theme	adolescentis	1173:1184	arg1	adolescentis					1173:1184	Bifidobacterium adolescentis	1157:1184	Bifidobacterium adolescentis (B.a.)	1157:1191	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	6	85	theme	adolescentis	1173:1184	arg1	group					932:936	control group	924:936	control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS	924:1133	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	6	85	theme	adolescentis	1173:1184	arg1	B.a.					1187:1190	B.a.	1187:1190	B.a.	1187:1190	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	6	85	theme	adolescentis	1173:1184	arg1	groups					889:894	three groups	883:894	three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days	883:1202	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	6	85	theme	adolescentis	1173:1184	arg1	CFU/ml					1147:1152	1 × 109 CFU/ml	1139:1152	1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.)	1139:1191	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	6	85	theme	adolescentis	1173:1184	arg1	mice					902:905	16 mice	899:905	16 mice	899:905	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	16	86	theme	bifidobacteria	2546:2559	arg1	number					2536:2541	the number	2532:2541	the number of bifidobacteria, which were able to mitigate intestinal immunopathology in the context of Pglyrp3 blockade	2532:2650	These manifestations of DSS-induced dysbiosis were significantly attenuated by feeding B.a. Both the local and systemic ill-being of the mice alleviated when they received B.a. DISCUSSION This study shows that Pglyrp3 facilitates recognition of bifidobacterial cell wall-derived peptidoglycan, thus leading additively to a reduction of metaflammation through an increase in the number of bifidobacteria, which were able to mitigate intestinal immunopathology in the context of Pglyrp3 blockade.
33242664	0	87	theme	Bifidobacterium	55:69	arg1	adolescentis					71:82	Bifidobacterium adolescentis	55:82	Bifidobacterium adolescentis	55:82	Study on the additive protective effect of PGLYRP3 and Bifidobacterium adolescentis Reuter 1963 on severity of DSS-induced colitis in Pglyrp3 knockout (Pglyrp3 -/-) and wild-type (WT) mice.
33242664	6	88	theme	sterile	1003:1009	arg1	water					1015:1019	sterile tap water	1003:1019	sterile tap water supplemented with only 5% DSS	1003:1049	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	4	89	theme	intestinal	727:736	arg1	inflammation					738:749	intestinal inflammation	727:749	intestinal inflammation	727:749	We investigated the extent to which Pglyrp3 and bifidobacteria regulate metaflammation and gut bacterial dysbiosis in DSS-induced mouse models of intestinal inflammation.
33242664	13	90	theme	-/-	1834:1836	arg1	mice					1838:1841	DSS-treated WT and DSS-treated Pglyrp3 -/- mice	1795:1841	DSS-treated WT and DSS-treated Pglyrp3 -/- mice	1795:1841	There was a significant difference in mean scores for inflammation severity between DSS-treated WT and DSS-treated Pglyrp3 -/- mice.
33242664	5	91	used	used	801:804	arg2	mice					791:794	MATERIAL & METHODS 8-10 weeks old male mice	752:794	MATERIAL & METHODS 8-10 weeks old male mice	752:794	MATERIAL & METHODS 8-10 weeks old male mice were used.
33242664	15	92	theme	-/-	2148:2150	arg1	mice					2152:2155	both WT and Pglyrp3 -/- mice	2128:2155	mice	2152:2155	qRT-PCR quantification showed that DSS significantly decreased the Clostridium coccoides and Bifidobacterium cell counts while increasing those of Bacteroides group in both WT and Pglyrp3 -/- mice.
33242664	5	93	theme	METHODS	763:769	arg1	mice					791:794	MATERIAL & METHODS 8-10 weeks old male mice	752:794	MATERIAL & METHODS 8-10 weeks old male mice	752:794	MATERIAL & METHODS 8-10 weeks old male mice were used.
33242664	6	94	dep	group	987:991	arg1	2					968:968	2	968:968	2	968:968	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	5	95	theme	male	786:789	arg1	mice					791:794	MATERIAL & METHODS 8-10 weeks old male mice	752:794	MATERIAL & METHODS 8-10 weeks old male mice	752:794	MATERIAL & METHODS 8-10 weeks old male mice were used.
33242664	12	96	theme	group	1636:1640	arg1	mice					1628:1631	mice	1628:1631	mice of group 2	1628:1642	RESULTS Intestinal inflammation in mice of group 2 were significantly increased compared to those of control group 1.
33242664	13	97	theme	WT	1807:1808	arg1	mice					1838:1841	DSS-treated WT and DSS-treated Pglyrp3 -/- mice	1795:1841	DSS-treated WT and DSS-treated Pglyrp3 -/- mice	1795:1841	There was a significant difference in mean scores for inflammation severity between DSS-treated WT and DSS-treated Pglyrp3 -/- mice.
33242664	16	98	dep	DISCUSSION	2335:2344	arg1	shows					2357:2361	shows	2357:2361	shows that Pglyrp3 facilitates recognition of bifidobacterial cell wall-derived peptidoglycan, thus leading additively to a reduction of metaflammation through an increase in the number of bifidobacteria, which were able to mitigate intestinal immunopathology in the context of Pglyrp3 blockade	2357:2650	These manifestations of DSS-induced dysbiosis were significantly attenuated by feeding B.a. Both the local and systemic ill-being of the mice alleviated when they received B.a. DISCUSSION This study shows that Pglyrp3 facilitates recognition of bifidobacterial cell wall-derived peptidoglycan, thus leading additively to a reduction of metaflammation through an increase in the number of bifidobacteria, which were able to mitigate intestinal immunopathology in the context of Pglyrp3 blockade.
33242664	0	99	from	effect	33:38	arg1	severity					99:106	severity	99:106	severity of DSS-induced colitis in Pglyrp3 knockout (Pglyrp3 -/-) and wild-type (WT) mice	99:187	Study on the additive protective effect of PGLYRP3 and Bifidobacterium adolescentis Reuter 1963 on severity of DSS-induced colitis in Pglyrp3 knockout (Pglyrp3 -/-) and wild-type (WT) mice.
33242664	13	100	theme	DSS-treated	1814:1824	arg1	mice					1838:1841	DSS-treated WT and DSS-treated Pglyrp3 -/- mice	1795:1841	DSS-treated WT and DSS-treated Pglyrp3 -/- mice	1795:1841	There was a significant difference in mean scores for inflammation severity between DSS-treated WT and DSS-treated Pglyrp3 -/- mice.
33242664	7	101	used	used	1275:1278	arg2	littermates					1220:1230	Wild-type (WT) littermates	1205:1230	Wild-type (WT) littermates of the respective gene (i.e. Pglyrp3)	1205:1268	Wild-type (WT) littermates of the respective gene (i.e. Pglyrp3) were used as controls throughout the study.
33242664	7	101	used	used	1275:1278	arg2	controls					1283:1290	controls	1283:1290	controls	1283:1290	Wild-type (WT) littermates of the respective gene (i.e. Pglyrp3) were used as controls throughout the study.
33242664	13	102	theme	inflammation	1765:1776	arg1	severity					1778:1785	inflammation severity	1765:1785	inflammation severity	1765:1785	There was a significant difference in mean scores for inflammation severity between DSS-treated WT and DSS-treated Pglyrp3 -/- mice.
33242664	0	103	theme	additive	13:20	arg1	effect					33:38	the additive protective effect	9:38	the additive protective effect of PGLYRP3 and Bifidobacterium adolescentis Reuter 1963 on severity of DSS-induced colitis in Pglyrp3 knockout (Pglyrp3 -/-) and wild-type (WT) mice	9:187	Study on the additive protective effect of PGLYRP3 and Bifidobacterium adolescentis Reuter 1963 on severity of DSS-induced colitis in Pglyrp3 knockout (Pglyrp3 -/-) and wild-type (WT) mice.
33242664	7	104	theme	respective	1239:1248	arg1	gene					1250:1253	the respective gene	1235:1253	the respective gene	1235:1253	Wild-type (WT) littermates of the respective gene (i.e. Pglyrp3) were used as controls throughout the study.
33242664	2	105	theme	marked	421:426	arg1	reduction					428:436	a marked reduction	419:436	a marked reduction of commensal bifidobacteria	419:464	Intestinal inflammation and metaflammation are commonly associated with a marked reduction of commensal bifidobacteria.
33242664	15	106	theme	qRT-PCR	1960:1966	arg1	quantification					1968:1981	qRT-PCR quantification	1960:1981	qRT-PCR quantification	1960:1981	qRT-PCR quantification showed that DSS significantly decreased the Clostridium coccoides and Bifidobacterium cell counts while increasing those of Bacteroides group in both WT and Pglyrp3 -/- mice.
33242664	0	107	dep	PGLYRP3	43:49	arg1	Reuter					84:89	Reuter	84:89	Reuter	84:89	Study on the additive protective effect of PGLYRP3 and Bifidobacterium adolescentis Reuter 1963 on severity of DSS-induced colitis in Pglyrp3 knockout (Pglyrp3 -/-) and wild-type (WT) mice.
33242664	8	108	theme	Clinical	1314:1321	arg1	signs					1323:1327	Clinical signs	1314:1327	Clinical signs of general health and inflammation	1314:1362	Clinical signs of general health and inflammation were monitored daily.
33242664	0	109	theme	PGLYRP3	43:49	arg1	effect					33:38	the additive protective effect	9:38	the additive protective effect of PGLYRP3 and Bifidobacterium adolescentis Reuter 1963 on severity of DSS-induced colitis in Pglyrp3 knockout (Pglyrp3 -/-) and wild-type (WT) mice	9:187	Study on the additive protective effect of PGLYRP3 and Bifidobacterium adolescentis Reuter 1963 on severity of DSS-induced colitis in Pglyrp3 knockout (Pglyrp3 -/-) and wild-type (WT) mice.
33242664	7	110	dep	Pglyrp3	1261:1267	arg1	i.e.					1256:1259	i.e.	1256:1259	i.e.	1256:1259	Wild-type (WT) littermates of the respective gene (i.e. Pglyrp3) were used as controls throughout the study.
33242664	6	111	theme	sterile	1092:1098	arg1	water					1104:1108	sterile tap water	1092:1108	sterile tap water supplemented with 5% DSS	1092:1133	In both WT and Pglyrp3 -/- experiments, the mice were randomly divided into three groups of 16 mice per group: (1) a control group receiving sterile tap water, (2) an experimental group receiving sterile tap water supplemented with only 5% DSS, and (3) an experimental group receiving sterile tap water supplemented with 5% DSS and 1 × 109 CFU/ml of Bifidobacterium adolescentis (B.a.) for 7 days.
33242664	2	112	theme	bifidobacteria	451:464	arg1	reduction					428:436	a marked reduction	419:436	a marked reduction of commensal bifidobacteria	419:464	Intestinal inflammation and metaflammation are commonly associated with a marked reduction of commensal bifidobacteria.
33242664	1	113	theme	gut	290:292	arg1	microbiome					294:303	the habitual gut microbiome	277:303	the habitual gut microbiome	277:303	BACKGROUND AND AIMS Pglyrp3 is a bactericidal innate immunity protein known to sustain the habitual gut microbiome and protect against experimental colitis.
33242664	15	114	theme	Bifidobacterium	2053:2067	arg1	counts					2074:2079	Bifidobacterium cell counts	2053:2079	Bifidobacterium cell counts	2053:2079	qRT-PCR quantification showed that DSS significantly decreased the Clostridium coccoides and Bifidobacterium cell counts while increasing those of Bacteroides group in both WT and Pglyrp3 -/- mice.
33242664	16	115	theme	wall-derived	2424:2435	arg1	peptidoglycan					2437:2449	bifidobacterial cell wall-derived peptidoglycan	2403:2449	bifidobacterial cell wall-derived peptidoglycan	2403:2449	These manifestations of DSS-induced dysbiosis were significantly attenuated by feeding B.a. Both the local and systemic ill-being of the mice alleviated when they received B.a. DISCUSSION This study shows that Pglyrp3 facilitates recognition of bifidobacterial cell wall-derived peptidoglycan, thus leading additively to a reduction of metaflammation through an increase in the number of bifidobacteria, which were able to mitigate intestinal immunopathology in the context of Pglyrp3 blockade.
33242664	8	116	theme	health	1340:1345	arg1	signs					1323:1327	Clinical signs	1314:1327	Clinical signs of general health and inflammation	1314:1362	Clinical signs of general health and inflammation were monitored daily.
33242664	16	117	theme	bifidobacterial	2403:2417	arg1	peptidoglycan					2437:2449	bifidobacterial cell wall-derived peptidoglycan	2403:2449	bifidobacterial cell wall-derived peptidoglycan	2403:2449	These manifestations of DSS-induced dysbiosis were significantly attenuated by feeding B.a. Both the local and systemic ill-being of the mice alleviated when they received B.a. DISCUSSION This study shows that Pglyrp3 facilitates recognition of bifidobacterial cell wall-derived peptidoglycan, thus leading additively to a reduction of metaflammation through an increase in the number of bifidobacteria, which were able to mitigate intestinal immunopathology in the context of Pglyrp3 blockade.
33242664	13	118	theme	mean	1749:1752	arg1	scores					1754:1759	mean scores	1749:1759	mean scores for inflammation severity	1749:1785	There was a significant difference in mean scores for inflammation severity between DSS-treated WT and DSS-treated Pglyrp3 -/- mice.
33242664	8	119	theme	inflammation	1351:1362	arg1	signs					1323:1327	Clinical signs	1314:1327	Clinical signs of general health and inflammation	1314:1362	Clinical signs of general health and inflammation were monitored daily.
33242664	11	120	theme	liver	1540:1544	arg1	inflammation					1546:1557	liver inflammation	1540:1557	liver inflammation	1540:1557	Metabolic parameters and liver inflammation were determined in serum samples.
32183253	0	0	theme	Genetic	77:83	arg1	Parameters					85:94	Genetic Parameters	77:94	Genetic Parameters	77:94	Integrating Casein Complex SNPs Additive, Dominance and Epistatic Effects on Genetic Parameters and Breeding Values Estimation for Murciano-Granadina Goat Milk Yield and Components.
32183253	4	1	theme	quantitative	994:1005	arg1	production					1028:1037	quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count)	994:1136	quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count)	994:1136	Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count).
32183253	7	2	theme	including	1455:1463	arg1	effects					1473:1479	including genetic effects	1455:1479	including genetic effects	1455:1479	Conclusively, including genetic effects and relationships among these heritable biomarkers may improve model efficiency, genetic parameters, and breeding values for milk yield and composition, optimizing selection practices profitability for components whose technological application may be especially relevant for the cheese-making dairy sector.
32183253	5	3	theme	somatic	1164:1170	arg1	count					1177:1181	somatic cell count	1164:1181	somatic cell count	1164:1181	Milk yield, lactose, and somatic cell count heritabilities increased considerably when the model including genetic effects was considered (0.46, 0.30, 0.43, respectively).
32183253	7	4	theme	milk	1606:1609	arg1	yield					1611:1615	milk yield	1606:1615	milk yield	1606:1615	Conclusively, including genetic effects and relationships among these heritable biomarkers may improve model efficiency, genetic parameters, and breeding values for milk yield and composition, optimizing selection practices profitability for components whose technological application may be especially relevant for the cheese-making dairy sector.
32183253	1	5	theme	traits	410:415	arg1	regulation					385:394	the genetic regulation	373:394	the genetic regulation of profitable traits	373:415	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	7	6	theme	breeding	1586:1593	arg1	values					1595:1600	breeding values	1586:1600	breeding values for milk yield and composition	1586:1631	Conclusively, including genetic effects and relationships among these heritable biomarkers may improve model efficiency, genetic parameters, and breeding values for milk yield and composition, optimizing selection practices profitability for components whose technological application may be especially relevant for the cheese-making dairy sector.
32183253	5	7	theme	genetic	1246:1252	arg1	effects					1254:1260	genetic effects	1246:1260	genetic effects	1246:1260	Milk yield, lactose, and somatic cell count heritabilities increased considerably when the model including genetic effects was considered (0.46, 0.30, 0.43, respectively).
32183253	4	8	from	outcomes	963:970	arg1	production					1028:1037	quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count)	994:1136	quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count)	994:1136	Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count).
32183253	4	9	theme	principal	791:799	arg1	analysis					811:818	categorical principal component analysis	779:818	categorical principal component analysis (CATPCA)	779:827	Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count).
32183253	4	9	theme	principal	791:799	arg1	CATPCA					821:826	CATPCA	821:826	CATPCA	821:826	Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count).
32183253	2	10	theme	breeding	581:588	arg1	values					590:595	breeding values	581:595	breeding values	581:595	Contextually, new genomic selection perspectives may translate this higher efficiency into higher accuracies for milk yield and components' genetic parameters and breeding values.
32183253	5	11	theme	count	1177:1181	arg1	heritabilities					1183:1196	Milk yield, lactose, and somatic cell count heritabilities	1139:1196	Milk yield, lactose, and somatic cell count heritabilities	1139:1196	Milk yield, lactose, and somatic cell count heritabilities increased considerably when the model including genetic effects was considered (0.46, 0.30, 0.43, respectively).
32183253	2	12	theme	higher	509:514	arg1	accuracies					516:525	higher accuracies	509:525	higher accuracies for milk yield and components' genetic parameters	509:575	Contextually, new genomic selection perspectives may translate this higher efficiency into higher accuracies for milk yield and components' genetic parameters and breeding values.
32183253	1	13	theme	epistatic	319:327	arg1	relationships					329:341	their epistatic relationships	313:341	their epistatic relationships	313:341	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	4	14	dep	yield	1063:1067	arg1	fat					1079:1081	fat	1079:1081	fat	1079:1081	Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count).
32183253	4	14	dep	yield	1063:1067	arg1	count					1131:1135	lactose contents and somatic cells count	1096:1135	lactose contents and somatic cells count	1096:1135	Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count).
32183253	4	14	dep	yield	1063:1067	arg1	protein					1070:1076	protein	1070:1076	protein	1070:1076	Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count).
32183253	4	14	dep	yield	1063:1067	arg1	solids					1084:1089	solids	1084:1089	solids	1084:1089	Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count).
32183253	4	15	theme	nonparametric	758:770	arg1	tests					772:776	Bonferroni-corrected nonparametric tests	737:776	Bonferroni-corrected nonparametric tests	737:776	Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count).
32183253	4	16	theme	somatic	1117:1123	arg1	cells					1125:1129	somatic cells	1117:1129	somatic cells	1117:1129	Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count).
32183253	4	17	theme	cells	1125:1129	arg1	count					1131:1135	lactose contents and somatic cells count	1096:1135	lactose contents and somatic cells count	1096:1135	Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count).
32183253	0	18	theme	Values	109:114	arg1	Estimation					116:125	Breeding Values Estimation	100:125	Breeding Values Estimation for Murciano-Granadina Goat Milk Yield and Components	100:179	Integrating Casein Complex SNPs Additive, Dominance and Epistatic Effects on Genetic Parameters and Breeding Values Estimation for Murciano-Granadina Goat Milk Yield and Components.
32183253	3	19	theme	casein	711:716	arg1	SNPs					731:734	48 casein loci-located SNPs	708:734	48 casein loci-located SNPs	708:734	A total of 2594 lactation records were collected from 159 Murciano-Granadina goats (2005-2018), genotyped for 48 casein loci-located SNPs.
32183253	4	20	theme	canonical	844:852	arg1	correlations					854:865	nonlinear canonical correlations	834:865	nonlinear canonical correlations	834:865	Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count).
32183253	4	21	theme	categorical	779:789	arg1	analysis					811:818	categorical principal component analysis	779:818	categorical principal component analysis (CATPCA)	779:827	Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count).
32183253	4	21	theme	categorical	779:789	arg1	CATPCA					821:826	CATPCA	821:826	CATPCA	821:826	Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count).
32183253	4	22	dep	traits	1039:1044	arg1	yield					1063:1067	yield	1063:1067	yield	1063:1067	Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count).
32183253	4	22	dep	traits	1039:1044	arg1	models					1055:1060	genetic models	1047:1060	quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count)	994:1136	Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count).
32183253	0	23	theme	Breeding	100:107	arg1	Estimation					116:125	Breeding Values Estimation	100:125	Breeding Values Estimation for Murciano-Granadina Goat Milk Yield and Components	100:179	Integrating Casein Complex SNPs Additive, Dominance and Epistatic Effects on Genetic Parameters and Breeding Values Estimation for Murciano-Granadina Goat Milk Yield and Components.
32183253	6	24	theme	standard	1322:1329	arg1	errors					1342:1347	Components standard prediction errors	1311:1347	Components standard prediction errors	1311:1347	Components standard prediction errors decreased, and accuracies and reliabilities increased when genetic effects were considered.
32183253	4	25	theme	nonlinear	834:842	arg1	correlations					854:865	nonlinear canonical correlations	834:865	nonlinear canonical correlations	834:865	Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count).
32183253	2	26	theme	Contextually	418:429	arg1	perspectives					454:465	Contextually, new genomic selection perspectives	418:465	perspectives	454:465	Contextually, new genomic selection perspectives may translate this higher efficiency into higher accuracies for milk yield and components' genetic parameters and breeding values.
32183253	7	27	theme	technological	1700:1712	arg1	application					1714:1724	application	1714:1724	application	1714:1724	Conclusively, including genetic effects and relationships among these heritable biomarkers may improve model efficiency, genetic parameters, and breeding values for milk yield and composition, optimizing selection practices profitability for components whose technological application may be especially relevant for the cheese-making dairy sector.
32183253	5	28	theme	Milk	1139:1142	arg1	yield					1144:1148	Milk yield	1139:1148	Milk yield	1139:1148	Milk yield, lactose, and somatic cell count heritabilities increased considerably when the model including genetic effects was considered (0.46, 0.30, 0.43, respectively).
32183253	0	29	theme	Casein	12:17	arg1	Complex					19:25	Integrating Casein Complex	0:25	Integrating Casein Complex	0:25	Integrating Casein Complex SNPs Additive, Dominance and Epistatic Effects on Genetic Parameters and Breeding Values Estimation for Murciano-Granadina Goat Milk Yield and Components.
32183253	4	30	theme	inclusion	981:989	arg1	outcomes					963:970	the outcomes	959:970	the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count)	959:1136	Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count).
32183253	2	31	theme	genetic	558:564	arg1	parameters					566:575	components' genetic parameters	546:575	components' genetic parameters	546:575	Contextually, new genomic selection perspectives may translate this higher efficiency into higher accuracies for milk yield and components' genetic parameters and breeding values.
32183253	7	32	theme	heritable	1511:1519	arg1	biomarkers					1521:1530	these heritable biomarkers	1505:1530	these heritable biomarkers	1505:1530	Conclusively, including genetic effects and relationships among these heritable biomarkers may improve model efficiency, genetic parameters, and breeding values for milk yield and composition, optimizing selection practices profitability for components whose technological application may be especially relevant for the cheese-making dairy sector.
32183253	0	33	theme	Integrating	0:10	arg1	Complex					19:25	Integrating Casein Complex	0:25	Integrating Casein Complex	0:25	Integrating Casein Complex SNPs Additive, Dominance and Epistatic Effects on Genetic Parameters and Breeding Values Estimation for Murciano-Granadina Goat Milk Yield and Components.
32183253	7	34	theme	model	1544:1548	arg1	efficiency					1550:1559	model efficiency	1544:1559	model efficiency	1544:1559	Conclusively, including genetic effects and relationships among these heritable biomarkers may improve model efficiency, genetic parameters, and breeding values for milk yield and composition, optimizing selection practices profitability for components whose technological application may be especially relevant for the cheese-making dairy sector.
32183253	1	35	theme	profitable	399:408	arg1	traits					410:415	profitable traits	399:415	profitable traits	399:415	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	3	36	theme	Murciano-Granadina	656:673	arg1	2005-2018					682:690	2005-2018	682:690	2005-2018	682:690	A total of 2594 lactation records were collected from 159 Murciano-Granadina goats (2005-2018), genotyped for 48 casein loci-located SNPs.
32183253	3	36	theme	Murciano-Granadina	656:673	arg1	goats					675:679	159 Murciano-Granadina goats	652:679	159 Murciano-Granadina goats (2005-2018)	652:691	A total of 2594 lactation records were collected from 159 Murciano-Granadina goats (2005-2018), genotyped for 48 casein loci-located SNPs.
32183253	5	37	dep	increased	1198:1206	arg1	0.46					1278:1281	0.46	1278:1281	0.46	1278:1281	Milk yield, lactose, and somatic cell count heritabilities increased considerably when the model including genetic effects was considered (0.46, 0.30, 0.43, respectively).
32183253	2	38	theme	milk	531:534	arg1	yield					536:540	milk yield	531:540	milk yield	531:540	Contextually, new genomic selection perspectives may translate this higher efficiency into higher accuracies for milk yield and components' genetic parameters and breeding values.
32183253	5	39	theme	lactose	1151:1157	arg1	heritabilities					1183:1196	Milk yield, lactose, and somatic cell count heritabilities	1139:1196	Milk yield, lactose, and somatic cell count heritabilities	1139:1196	Milk yield, lactose, and somatic cell count heritabilities increased considerably when the model including genetic effects was considered (0.46, 0.30, 0.43, respectively).
32183253	4	40	theme	epistatic	928:936	arg1	effects					938:944	additive, dominance, and interSNP epistatic effects	894:944	effects	938:944	Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count).
32183253	6	41	theme	Components	1311:1320	arg1	errors					1342:1347	Components standard prediction errors	1311:1347	Components standard prediction errors	1311:1347	Components standard prediction errors decreased, and accuracies and reliabilities increased when genetic effects were considered.
32183253	4	42	theme	Bonferroni-corrected	737:756	arg1	tests					772:776	Bonferroni-corrected nonparametric tests	737:776	Bonferroni-corrected nonparametric tests	737:776	Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count).
32183253	1	43	theme	additive	206:213	arg1	effects					215:221	additive effects	206:221	additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein)	206:306	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	4	44	theme	genetic	1047:1053	arg1	models					1055:1060	genetic models	1047:1060	quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count)	994:1136	Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count).
32183253	3	45	theme	loci-located	718:729	arg1	SNPs					731:734	48 casein loci-located SNPs	708:734	48 casein loci-located SNPs	708:734	A total of 2594 lactation records were collected from 159 Murciano-Granadina goats (2005-2018), genotyped for 48 casein loci-located SNPs.
32183253	4	46	theme	interSNP	919:926	arg1	effects					938:944	additive, dominance, and interSNP epistatic effects	894:944	effects	938:944	Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count).
32183253	1	47	dep	polymorphisms	259:271	arg1	polymorphisms					259:271	casein complex single-nucleotide polymorphisms	226:271	casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein)	226:306	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	1	47	dep	polymorphisms	259:271	arg1	β					291:291	β	291:291	β	291:291	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	1	47	dep	polymorphisms	259:271	arg1	αS2					286:288	αS2	286:288	αS2	286:288	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	1	47	dep	polymorphisms	259:271	arg1	αS1					281:283	αS1	281:283	αS1	281:283	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	1	47	dep	polymorphisms	259:271	arg1	casein					300:305	κ casein	298:305	κ casein	298:305	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	2	48	theme	selection	444:452	arg1	perspectives					454:465	Contextually, new genomic selection perspectives	418:465	perspectives	454:465	Contextually, new genomic selection perspectives may translate this higher efficiency into higher accuracies for milk yield and components' genetic parameters and breeding values.
32183253	4	49	theme	contents	1104:1111	arg1	count					1131:1135	lactose contents and somatic cells count	1096:1135	lactose contents and somatic cells count	1096:1135	Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count).
32183253	4	50	theme	qualitative	1011:1021	arg1	production					1028:1037	quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count)	994:1136	quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count)	994:1136	Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count).
32183253	0	51	theme	Goat	150:153	arg1	Yield					160:164	Murciano-Granadina Goat Milk Yield	131:164	Murciano-Granadina Goat Milk Yield	131:164	Integrating Casein Complex SNPs Additive, Dominance and Epistatic Effects on Genetic Parameters and Breeding Values Estimation for Murciano-Granadina Goat Milk Yield and Components.
32183253	4	52	theme	lactose	1096:1102	arg1	contents					1104:1111	lactose contents	1096:1111	lactose contents	1096:1111	Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count).
32183253	5	53	theme	cell	1172:1175	arg1	count					1177:1181	somatic cell count	1164:1181	somatic cell count	1164:1181	Milk yield, lactose, and somatic cell count heritabilities increased considerably when the model including genetic effects was considered (0.46, 0.30, 0.43, respectively).
32183253	0	54	theme	Additive	32:39	arg1	Effects					66:72	Additive, Dominance and Epistatic Effects	32:72	Additive, Dominance and Epistatic Effects on Genetic Parameters and Breeding Values Estimation for Murciano-Granadina Goat Milk Yield and Components	32:179	Integrating Casein Complex SNPs Additive, Dominance and Epistatic Effects on Genetic Parameters and Breeding Values Estimation for Murciano-Granadina Goat Milk Yield and Components.
32183253	4	55	theme	milk	1023:1026	arg1	production					1028:1037	quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count)	994:1136	quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count)	994:1136	Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count).
32183253	0	56	theme	Murciano-Granadina	131:148	arg1	Yield					160:164	Murciano-Granadina Goat Milk Yield	131:164	Murciano-Granadina Goat Milk Yield	131:164	Integrating Casein Complex SNPs Additive, Dominance and Epistatic Effects on Genetic Parameters and Breeding Values Estimation for Murciano-Granadina Goat Milk Yield and Components.
32183253	0	57	from	Effects	66:72	arg1	Estimation					116:125	Breeding Values Estimation	100:125	Breeding Values Estimation for Murciano-Granadina Goat Milk Yield and Components	100:179	Integrating Casein Complex SNPs Additive, Dominance and Epistatic Effects on Genetic Parameters and Breeding Values Estimation for Murciano-Granadina Goat Milk Yield and Components.
32183253	0	57	from	Effects	66:72	arg1	Parameters					85:94	Genetic Parameters	77:94	Genetic Parameters	77:94	Integrating Casein Complex SNPs Additive, Dominance and Epistatic Effects on Genetic Parameters and Breeding Values Estimation for Murciano-Granadina Goat Milk Yield and Components.
32183253	7	58	theme	genetic	1465:1471	arg1	effects					1473:1479	including genetic effects	1455:1479	including genetic effects	1455:1479	Conclusively, including genetic effects and relationships among these heritable biomarkers may improve model efficiency, genetic parameters, and breeding values for milk yield and composition, optimizing selection practices profitability for components whose technological application may be especially relevant for the cheese-making dairy sector.
32183253	1	59	from	knowledge	360:368	arg1	regulation					385:394	the genetic regulation	373:394	the genetic regulation of profitable traits	373:415	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	2	60	theme	genomic	436:442	arg1	perspectives					454:465	Contextually, new genomic selection perspectives	418:465	perspectives	454:465	Contextually, new genomic selection perspectives may translate this higher efficiency into higher accuracies for milk yield and components' genetic parameters and breeding values.
32183253	4	61	theme	additive	894:901	arg1	dominance					904:912	additive, dominance, and interSNP epistatic effects	894:944	dominance	904:912	Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count).
32183253	3	62	theme	records	624:630	arg1	total					600:604	A total	598:604	A total of 2594 lactation records	598:630	A total of 2594 lactation records were collected from 159 Murciano-Granadina goats (2005-2018), genotyped for 48 casein loci-located SNPs.
32183253	7	63	theme	cheese-making	1761:1773	arg1	sector					1781:1786	the cheese-making dairy sector	1757:1786	the cheese-making dairy sector	1757:1786	Conclusively, including genetic effects and relationships among these heritable biomarkers may improve model efficiency, genetic parameters, and breeding values for milk yield and composition, optimizing selection practices profitability for components whose technological application may be especially relevant for the cheese-making dairy sector.
32183253	2	64	theme	new	432:434	arg1	perspectives					454:465	Contextually, new genomic selection perspectives	418:465	perspectives	454:465	Contextually, new genomic selection perspectives may translate this higher efficiency into higher accuracies for milk yield and components' genetic parameters and breeding values.
32183253	1	65	theme	κ	298:298	arg1	polymorphisms					259:271	casein complex single-nucleotide polymorphisms	226:271	casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein)	226:306	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	1	65	theme	κ	298:298	arg1	casein					300:305	κ casein	298:305	κ casein	298:305	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	0	66	theme	Milk	155:158	arg1	Yield					160:164	Murciano-Granadina Goat Milk Yield	131:164	Murciano-Granadina Goat Milk Yield	131:164	Integrating Casein Complex SNPs Additive, Dominance and Epistatic Effects on Genetic Parameters and Breeding Values Estimation for Murciano-Granadina Goat Milk Yield and Components.
32183253	1	67	theme	genetic	377:383	arg1	regulation					385:394	the genetic regulation	373:394	the genetic regulation of profitable traits	373:415	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	0	68	dep	Additive	32:39	arg1	Epistatic					56:64	Epistatic	56:64	Epistatic	56:64	Integrating Casein Complex SNPs Additive, Dominance and Epistatic Effects on Genetic Parameters and Breeding Values Estimation for Murciano-Granadina Goat Milk Yield and Components.
32183253	0	68	dep	Additive	32:39	arg1	Dominance					42:50	Dominance	42:50	Dominance	42:50	Integrating Casein Complex SNPs Additive, Dominance and Epistatic Effects on Genetic Parameters and Breeding Values Estimation for Murciano-Granadina Goat Milk Yield and Components.
32183253	7	69	theme	profitability	1665:1677	arg1	practices					1655:1663	selection practices	1645:1663	selection practices profitability for components whose technological application may be especially relevant for the cheese-making dairy sector	1645:1786	Conclusively, including genetic effects and relationships among these heritable biomarkers may improve model efficiency, genetic parameters, and breeding values for milk yield and composition, optimizing selection practices profitability for components whose technological application may be especially relevant for the cheese-making dairy sector.
32183253	3	70	theme	lactation	614:622	arg1	records					624:630	2594 lactation records	609:630	2594 lactation records	609:630	A total of 2594 lactation records were collected from 159 Murciano-Granadina goats (2005-2018), genotyped for 48 casein loci-located SNPs.
32183253	5	71	theme	yield	1144:1148	arg1	heritabilities					1183:1196	Milk yield, lactose, and somatic cell count heritabilities	1139:1196	Milk yield, lactose, and somatic cell count heritabilities	1139:1196	Milk yield, lactose, and somatic cell count heritabilities increased considerably when the model including genetic effects was considered (0.46, 0.30, 0.43, respectively).
32183253	4	72	theme	component	801:809	arg1	analysis					811:818	categorical principal component analysis	779:818	categorical principal component analysis (CATPCA)	779:827	Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count).
32183253	4	72	theme	component	801:809	arg1	CATPCA					821:826	CATPCA	821:826	CATPCA	821:826	Bonferroni-corrected nonparametric tests, categorical principal component analysis (CATPCA), and nonlinear canonical correlations were performed to quantify additive, dominance, and interSNP epistatic effects and evaluate the outcomes of their inclusion in quantitative and qualitative milk production traits' genetic models (yield, protein, fat, solids, and lactose contents and somatic cells count).
32183253	1	73	theme	complex	233:239	arg1	polymorphisms					259:271	casein complex single-nucleotide polymorphisms	226:271	casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein)	226:306	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	1	73	theme	complex	233:239	arg1	αS1					281:283	αS1	281:283	αS1	281:283	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	1	73	theme	complex	233:239	arg1	αS2					286:288	αS2	286:288	αS2	286:288	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	1	73	theme	complex	233:239	arg1	SNPs					274:277	SNPs	274:277	SNPs	274:277	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	1	73	theme	complex	233:239	arg1	casein					300:305	κ casein	298:305	κ casein	298:305	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	7	74	theme	selection	1645:1653	arg1	practices					1655:1663	selection practices	1645:1663	selection practices profitability for components whose technological application may be especially relevant for the cheese-making dairy sector	1645:1786	Conclusively, including genetic effects and relationships among these heritable biomarkers may improve model efficiency, genetic parameters, and breeding values for milk yield and composition, optimizing selection practices profitability for components whose technological application may be especially relevant for the cheese-making dairy sector.
32183253	3	75	theme	2594	609:612	arg1	records					624:630	2594 lactation records	609:630	2594 lactation records	609:630	A total of 2594 lactation records were collected from 159 Murciano-Granadina goats (2005-2018), genotyped for 48 casein loci-located SNPs.
32183253	6	76	theme	prediction	1331:1340	arg1	errors					1342:1347	Components standard prediction errors	1311:1347	Components standard prediction errors	1311:1347	Components standard prediction errors decreased, and accuracies and reliabilities increased when genetic effects were considered.
32183253	1	77	theme	single-nucleotide	241:257	arg1	polymorphisms					259:271	casein complex single-nucleotide polymorphisms	226:271	casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein)	226:306	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	1	77	theme	single-nucleotide	241:257	arg1	αS1					281:283	αS1	281:283	αS1	281:283	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	1	77	theme	single-nucleotide	241:257	arg1	αS2					286:288	αS2	286:288	αS2	286:288	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	1	77	theme	single-nucleotide	241:257	arg1	SNPs					274:277	SNPs	274:277	SNPs	274:277	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	1	77	theme	single-nucleotide	241:257	arg1	casein					300:305	κ casein	298:305	κ casein	298:305	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	2	78	theme	higher	486:491	arg1	efficiency					493:502	this higher efficiency	481:502	this higher efficiency	481:502	Contextually, new genomic selection perspectives may translate this higher efficiency into higher accuracies for milk yield and components' genetic parameters and breeding values.
32183253	1	79	theme	casein	226:231	arg1	polymorphisms					259:271	casein complex single-nucleotide polymorphisms	226:271	casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein)	226:306	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	1	79	theme	casein	226:231	arg1	αS1					281:283	αS1	281:283	αS1	281:283	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	1	79	theme	casein	226:231	arg1	αS2					286:288	αS2	286:288	αS2	286:288	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	1	79	theme	casein	226:231	arg1	SNPs					274:277	SNPs	274:277	SNPs	274:277	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	1	79	theme	casein	226:231	arg1	casein					300:305	κ casein	298:305	κ casein	298:305	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	7	80	theme	genetic	1562:1568	arg1	parameters					1570:1579	genetic parameters	1562:1579	genetic parameters	1562:1579	Conclusively, including genetic effects and relationships among these heritable biomarkers may improve model efficiency, genetic parameters, and breeding values for milk yield and composition, optimizing selection practices profitability for components whose technological application may be especially relevant for the cheese-making dairy sector.
32183253	7	81	theme	dairy	1775:1779	arg1	sector					1781:1786	the cheese-making dairy sector	1757:1786	the cheese-making dairy sector	1757:1786	Conclusively, including genetic effects and relationships among these heritable biomarkers may improve model efficiency, genetic parameters, and breeding values for milk yield and composition, optimizing selection practices profitability for components whose technological application may be especially relevant for the cheese-making dairy sector.
32183253	1	82	theme	polymorphisms	259:271	arg1	dominance					192:200	dominance	192:200	dominance	192:200	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	1	82	theme	polymorphisms	259:271	arg1	effects					215:221	additive effects	206:221	additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein)	206:306	Assessing dominance and additive effects of casein complex single-nucleotide polymorphisms (SNPs) (αS1, αS2, β, and κ casein), and their epistatic relationships may maximize our knowledge on the genetic regulation of profitable traits.
32183253	6	83	theme	genetic	1408:1414	arg1	effects					1416:1422	genetic effects	1408:1422	genetic effects	1408:1422	Components standard prediction errors decreased, and accuracies and reliabilities increased when genetic effects were considered.
32183253	3	84	dep	collected	637:645	arg1	genotyped					694:702	genotyped	694:702	genotyped for 48 casein loci-located SNPs	694:734	A total of 2594 lactation records were collected from 159 Murciano-Granadina goats (2005-2018), genotyped for 48 casein loci-located SNPs.
33381584	8	0	theme	RNA	965:967	arg1	RNAi					983:986	RNAi	983:986	RNAi	983:986	After RNA interference (RNAi), IκBα mRNA and protein expression were significantly inhibited.
33381584	8	0	theme	RNA	965:967	arg1	interference					969:980	RNA interference	965:980	RNA interference (RNAi)	965:987	After RNA interference (RNAi), IκBα mRNA and protein expression were significantly inhibited.
33381584	13	1	theme	ciliary	1435:1441	arg1	muscle					1443:1448	the ciliary muscle	1431:1448	the ciliary muscle of the DMAPA-Glyp+siRNA group	1431:1478	The levels of Cy3-siRNA in the ciliary muscle of the DMAPA-Glyp+siRNA group did not significantly decrease over time.
33381584	7	2	theme	Gene	867:870	arg1	efficiency					885:894	Gene transfection efficiency	867:894	Gene transfection efficiency	867:894	Gene transfection efficiency and toxicity of the DMAPA-Glyp derivative were also evaluated.
33381584	17	3	theme	siRNA	1969:1973	arg1	vector					1975:1980	an effective siRNA vector	1956:1980	an effective siRNA vector in rat eyes	1956:1992	The DMAPA-Glyp derivative is safe and feasible as an effective siRNA vector in rat eyes.
33381584	15	4	theme	rats	1831:1834	arg1	values					1821:1826	the IOP values	1813:1826	the IOP values of rats	1813:1834	Taken together, our results suggest that downregulation of IκBα expression in the ciliary muscle plays a crucial role in reducing the IOP values of rats.
33381584	6	5	theme	intraocular	802:812	arg1	IOP					824:826	IOP	824:826	IOP	824:826	Rat intraocular pressure (IOP) was monitored pre- and postinjection.
33381584	6	5	theme	intraocular	802:812	arg1	pressure					814:821	Rat intraocular pressure	798:821	Rat intraocular pressure (IOP)	798:827	Rat intraocular pressure (IOP) was monitored pre- and postinjection.
33381584	15	6	from	downregulation	1724:1737	arg1	muscle					1773:1778	the ciliary muscle	1761:1778	the ciliary muscle	1761:1778	Taken together, our results suggest that downregulation of IκBα expression in the ciliary muscle plays a crucial role in reducing the IOP values of rats.
33381584	13	7	from	levels	1408:1413	arg1	muscle					1443:1448	the ciliary muscle	1431:1448	the ciliary muscle of the DMAPA-Glyp+siRNA group	1431:1478	The levels of Cy3-siRNA in the ciliary muscle of the DMAPA-Glyp+siRNA group did not significantly decrease over time.
33381584	1	8	theme	outflow	186:192	arg1	pathway					194:200	the uveoscleral outflow pathway	170:200	the uveoscleral outflow pathway	170:200	The role of the IκB/NF-κB signaling pathway in the uveoscleral outflow pathway was investigated with IκBα gene silencing mediated by the 3-(dimethylamino)-1-propylamine-conjugated glycogen (DMAPA-Glyp) derivative.
33381584	8	9	theme	IκBα	990:993	arg1	mRNA					995:998	IκBα mRNA	990:998	IκBα mRNA	990:998	After RNA interference (RNAi), IκBα mRNA and protein expression were significantly inhibited.
33381584	4	10	theme	real-time	663:671	arg1	PCR					673:675	real-time PCR	663:675	real-time PCR	663:675	The mRNA and protein expression of IκBα, NF-κBp65, and MMP-2 were analyzed by real-time PCR, western blotting, and in situ gelatin zymography.
33381584	16	11	theme	molecular	1859:1867	arg1	target					1869:1874	a new molecular target	1853:1874	a new molecular target for lowering IOP in glaucoma	1853:1903	IκBα may become a new molecular target for lowering IOP in glaucoma.
33381584	1	12	from	role	127:130	arg1	pathway					194:200	the uveoscleral outflow pathway	170:200	the uveoscleral outflow pathway	170:200	The role of the IκB/NF-κB signaling pathway in the uveoscleral outflow pathway was investigated with IκBα gene silencing mediated by the 3-(dimethylamino)-1-propylamine-conjugated glycogen (DMAPA-Glyp) derivative.
33381584	12	13	theme	lowest	1370:1375	arg1	level					1377:1381	the lowest level	1366:1381	the lowest level on day 3	1366:1390	IOP in the DMAPA-Glyp+siRNA group fell to the lowest level on day 3 after RNAi.
33381584	4	14	theme	MMP-2	640:644	arg1	protein					598:604	protein	598:604	protein	598:604	The mRNA and protein expression of IκBα, NF-κBp65, and MMP-2 were analyzed by real-time PCR, western blotting, and in situ gelatin zymography.
33381584	4	14	theme	MMP-2	640:644	arg1	mRNA					589:592	mRNA	589:592	mRNA	589:592	The mRNA and protein expression of IκBα, NF-κBp65, and MMP-2 were analyzed by real-time PCR, western blotting, and in situ gelatin zymography.
33381584	4	15	dep	mRNA	589:592	arg1	The					585:587	The	585:587	The	585:587	The mRNA and protein expression of IκBα, NF-κBp65, and MMP-2 were analyzed by real-time PCR, western blotting, and in situ gelatin zymography.
33381584	4	15	dep	mRNA	589:592	arg1	expression					606:615	expression	606:615	expression	606:615	The mRNA and protein expression of IκBα, NF-κBp65, and MMP-2 were analyzed by real-time PCR, western blotting, and in situ gelatin zymography.
33381584	1	16	theme	gene	229:232	arg1	silencing					234:242	IκBα gene silencing	224:242	IκBα gene silencing mediated by the 3-(dimethylamino)-1-propylamine-conjugated glycogen (DMAPA-Glyp) derivative	224:334	The role of the IκB/NF-κB signaling pathway in the uveoscleral outflow pathway was investigated with IκBα gene silencing mediated by the 3-(dimethylamino)-1-propylamine-conjugated glycogen (DMAPA-Glyp) derivative.
33381584	15	17	theme	ciliary	1765:1771	arg1	muscle					1773:1778	the ciliary muscle	1761:1778	the ciliary muscle	1761:1778	Taken together, our results suggest that downregulation of IκBα expression in the ciliary muscle plays a crucial role in reducing the IOP values of rats.
33381584	1	18	theme	IκB/NF-κB	139:147	arg1	pathway					159:165	the IκB/NF-κB signaling pathway	135:165	the IκB/NF-κB signaling pathway	135:165	The role of the IκB/NF-κB signaling pathway in the uveoscleral outflow pathway was investigated with IκBα gene silencing mediated by the 3-(dimethylamino)-1-propylamine-conjugated glycogen (DMAPA-Glyp) derivative.
33381584	14	19	theme	DMAPA-Glyp/siRNA	1657:1672	arg1	complex					1674:1680	the DMAPA-Glyp/siRNA complex	1653:1680	the DMAPA-Glyp/siRNA complex	1653:1680	At 7 and 14 d after RNAi, no significant pathological damage was detectable in the eyes injected with the DMAPA-Glyp derivative or the DMAPA-Glyp/siRNA complex.
33381584	1	20	theme	pathway	159:165	arg1	role					127:130	The role	123:130	The role of the IκB/NF-κB signaling pathway in the uveoscleral outflow pathway	123:200	The role of the IκB/NF-κB signaling pathway in the uveoscleral outflow pathway was investigated with IκBα gene silencing mediated by the 3-(dimethylamino)-1-propylamine-conjugated glycogen (DMAPA-Glyp) derivative.
33381584	11	21	theme	DMAPA-Glyp+siRNA	1300:1315	arg1	group					1317:1321	the DMAPA-Glyp+siRNA group	1296:1321	the DMAPA-Glyp+siRNA group	1296:1321	Both mRNA expression and activity of MMP-2 increased, with the largest increase in the DMAPA-Glyp+siRNA group.
33381584	14	22	theme	significant	1551:1561	arg1	damage					1576:1581	no significant pathological damage	1548:1581	no significant pathological damage	1548:1581	At 7 and 14 d after RNAi, no significant pathological damage was detectable in the eyes injected with the DMAPA-Glyp derivative or the DMAPA-Glyp/siRNA complex.
33381584	9	23	theme	NF-κBp65	1053:1060	arg1	mRNA					1062:1065	NF-κBp65 mRNA	1053:1065	NF-κBp65 mRNA	1053:1065	NF-κBp65 mRNA and protein expression showed no significant differences.
33381584	0	24	theme	Outflow	98:104	arg1	Pathway					106:112	the Uveoscleral Outflow Pathway	82:112	the Uveoscleral Outflow Pathway in Rats	82:120	Hyperbranched Cationic Glycogen Derivative-Mediated IκBα Gene Silencing Regulates the Uveoscleral Outflow Pathway in Rats.
33381584	14	25	theme	DMAPA-Glyp	1628:1637	arg1	derivative					1639:1648	the DMAPA-Glyp derivative	1624:1648	the DMAPA-Glyp derivative	1624:1648	At 7 and 14 d after RNAi, no significant pathological damage was detectable in the eyes injected with the DMAPA-Glyp derivative or the DMAPA-Glyp/siRNA complex.
33381584	5	26	theme	Nuclear	728:734	arg1	translocation					736:748	Nuclear translocation	728:748	Nuclear translocation of NF-κBp65	728:760	Nuclear translocation of NF-κBp65 was analyzed by immunofluorescence.
33381584	14	27	from	7	1525:1525	arg1	detectable					1587:1596	detectable	1587:1596	detectable	1587:1596	At 7 and 14 d after RNAi, no significant pathological damage was detectable in the eyes injected with the DMAPA-Glyp derivative or the DMAPA-Glyp/siRNA complex.
33381584	0	28	theme	Hyperbranched	0:12	arg1	Silencing					62:70	Hyperbranched Cationic Glycogen Derivative-Mediated IκBα Gene Silencing	0:70	Hyperbranched Cationic Glycogen Derivative-Mediated IκBα Gene Silencing	0:70	Hyperbranched Cationic Glycogen Derivative-Mediated IκBα Gene Silencing Regulates the Uveoscleral Outflow Pathway in Rats.
33381584	1	29	theme	-1-propylamine-conjugated	277:301	arg1	derivative					325:334	the 3-(dimethylamino)-1-propylamine-conjugated glycogen (DMAPA-Glyp) derivative	256:334	the 3-(dimethylamino)-1-propylamine-conjugated glycogen (DMAPA-Glyp) derivative	256:334	The role of the IκB/NF-κB signaling pathway in the uveoscleral outflow pathway was investigated with IκBα gene silencing mediated by the 3-(dimethylamino)-1-propylamine-conjugated glycogen (DMAPA-Glyp) derivative.
33381584	7	30	theme	derivative	927:936	arg1	efficiency					885:894	Gene transfection efficiency	867:894	Gene transfection efficiency	867:894	Gene transfection efficiency and toxicity of the DMAPA-Glyp derivative were also evaluated.
33381584	7	30	theme	derivative	927:936	arg1	toxicity					900:907	toxicity	900:907	toxicity	900:907	Gene transfection efficiency and toxicity of the DMAPA-Glyp derivative were also evaluated.
33381584	4	31	dep	in	700:701	arg1	situ					703:706	situ	703:706	situ	703:706	The mRNA and protein expression of IκBα, NF-κBp65, and MMP-2 were analyzed by real-time PCR, western blotting, and in situ gelatin zymography.
33381584	6	32	theme	Rat	798:800	arg1	IOP					824:826	IOP	824:826	IOP	824:826	Rat intraocular pressure (IOP) was monitored pre- and postinjection.
33381584	6	32	theme	Rat	798:800	arg1	pressure					814:821	Rat intraocular pressure	798:821	Rat intraocular pressure (IOP)	798:827	Rat intraocular pressure (IOP) was monitored pre- and postinjection.
33381584	14	33	from	14 d	1531:1534	arg1	detectable					1587:1596	detectable	1587:1596	detectable	1587:1596	At 7 and 14 d after RNAi, no significant pathological damage was detectable in the eyes injected with the DMAPA-Glyp derivative or the DMAPA-Glyp/siRNA complex.
33381584	15	34	theme	crucial	1788:1794	arg1	role					1796:1799	a crucial role	1786:1799	a crucial role	1786:1799	Taken together, our results suggest that downregulation of IκBα expression in the ciliary muscle plays a crucial role in reducing the IOP values of rats.
33381584	13	35	theme	group	1474:1478	arg1	muscle					1443:1448	the ciliary muscle	1431:1448	the ciliary muscle of the DMAPA-Glyp+siRNA group	1431:1478	The levels of Cy3-siRNA in the ciliary muscle of the DMAPA-Glyp+siRNA group did not significantly decrease over time.
33381584	0	36	theme	Glycogen	23:30	arg1	Silencing					62:70	Hyperbranched Cationic Glycogen Derivative-Mediated IκBα Gene Silencing	0:70	Hyperbranched Cationic Glycogen Derivative-Mediated IκBα Gene Silencing	0:70	Hyperbranched Cationic Glycogen Derivative-Mediated IκBα Gene Silencing Regulates the Uveoscleral Outflow Pathway in Rats.
33381584	10	37	theme	nuclear	1139:1145	arg1	translocation					1147:1159	nuclear translocation	1139:1159	nuclear translocation of NF-κBp65	1139:1171	Nevertheless, nuclear translocation of NF-κBp65 occurred in the DMAPA-Glyp+siRNA group.
33381584	2	38	dep	injection	443:451	arg1	labeled					454:460	labeled	454:460	labeled as the DMAPA-Glyp+siRNA group	454:490	The IκBα-siRNA-loaded DMAPA-Glyp complex was transfected into the ciliary muscles of rats by intracameral injection (labeled as the DMAPA-Glyp+siRNA group).
33381584	16	39	theme	new	1855:1857	arg1	target					1869:1874	a new molecular target	1853:1874	a new molecular target for lowering IOP in glaucoma	1853:1903	IκBα may become a new molecular target for lowering IOP in glaucoma.
33381584	0	40	theme	IκBα	52:55	arg1	Silencing					62:70	Hyperbranched Cationic Glycogen Derivative-Mediated IκBα Gene Silencing	0:70	Hyperbranched Cationic Glycogen Derivative-Mediated IκBα Gene Silencing	0:70	Hyperbranched Cationic Glycogen Derivative-Mediated IκBα Gene Silencing Regulates the Uveoscleral Outflow Pathway in Rats.
33381584	11	41	theme	largest	1276:1282	arg1	increase					1284:1291	the largest increase	1272:1291	the largest increase in the DMAPA-Glyp+siRNA group	1272:1321	Both mRNA expression and activity of MMP-2 increased, with the largest increase in the DMAPA-Glyp+siRNA group.
33381584	14	42	from	eyes	1605:1608	arg1	detectable					1587:1596	detectable	1587:1596	detectable	1587:1596	At 7 and 14 d after RNAi, no significant pathological damage was detectable in the eyes injected with the DMAPA-Glyp derivative or the DMAPA-Glyp/siRNA complex.
33381584	3	43	theme	Lipo	519:522	arg1	complex					531:537	The Lipofectamine™ 2000 (Lipo)/siRNA complex	494:537	The Lipofectamine™ 2000 (Lipo)/siRNA complex	494:537	The Lipofectamine™ 2000 (Lipo)/siRNA complex and the naked siRNA were set as the controls.
33381584	3	43	theme	Lipo	519:522	arg1	controls					575:582	the controls	571:582	the controls	571:582	The Lipofectamine™ 2000 (Lipo)/siRNA complex and the naked siRNA were set as the controls.
33381584	3	43	theme	Lipo	519:522	arg1	siRNA					553:557	the naked siRNA	543:557	the naked siRNA	543:557	The Lipofectamine™ 2000 (Lipo)/siRNA complex and the naked siRNA were set as the controls.
33381584	2	44	theme	DMAPA-Glyp	359:368	arg1	complex					370:376	The IκBα-siRNA-loaded DMAPA-Glyp complex	337:376	The IκBα-siRNA-loaded DMAPA-Glyp complex	337:376	The IκBα-siRNA-loaded DMAPA-Glyp complex was transfected into the ciliary muscles of rats by intracameral injection (labeled as the DMAPA-Glyp+siRNA group).
33381584	7	45	theme	DMAPA-Glyp	916:925	arg1	derivative					927:936	the DMAPA-Glyp derivative	912:936	the DMAPA-Glyp derivative	912:936	Gene transfection efficiency and toxicity of the DMAPA-Glyp derivative were also evaluated.
33381584	16	46	from	IOP	1889:1891	arg1	glaucoma					1896:1903	glaucoma	1896:1903	glaucoma	1896:1903	IκBα may become a new molecular target for lowering IOP in glaucoma.
33381584	2	47	theme	ciliary	403:409	arg1	muscles					411:417	the ciliary muscles	399:417	the ciliary muscles of rats	399:425	The IκBα-siRNA-loaded DMAPA-Glyp complex was transfected into the ciliary muscles of rats by intracameral injection (labeled as the DMAPA-Glyp+siRNA group).
33381584	4	48	theme	IκBα	620:623	arg1	protein					598:604	protein	598:604	protein	598:604	The mRNA and protein expression of IκBα, NF-κBp65, and MMP-2 were analyzed by real-time PCR, western blotting, and in situ gelatin zymography.
33381584	4	48	theme	IκBα	620:623	arg1	mRNA					589:592	mRNA	589:592	mRNA	589:592	The mRNA and protein expression of IκBα, NF-κBp65, and MMP-2 were analyzed by real-time PCR, western blotting, and in situ gelatin zymography.
33381584	3	49	theme	/siRNA	524:529	arg1	complex					531:537	The Lipofectamine™ 2000 (Lipo)/siRNA complex	494:537	The Lipofectamine™ 2000 (Lipo)/siRNA complex	494:537	The Lipofectamine™ 2000 (Lipo)/siRNA complex and the naked siRNA were set as the controls.
33381584	3	49	theme	/siRNA	524:529	arg1	controls					575:582	the controls	571:582	the controls	571:582	The Lipofectamine™ 2000 (Lipo)/siRNA complex and the naked siRNA were set as the controls.
33381584	3	49	theme	/siRNA	524:529	arg1	siRNA					553:557	the naked siRNA	543:557	the naked siRNA	543:557	The Lipofectamine™ 2000 (Lipo)/siRNA complex and the naked siRNA were set as the controls.
33381584	17	50	theme	effective	1959:1967	arg1	vector					1975:1980	an effective siRNA vector	1956:1980	an effective siRNA vector in rat eyes	1956:1992	The DMAPA-Glyp derivative is safe and feasible as an effective siRNA vector in rat eyes.
33381584	13	51	theme	Cy3-siRNA	1418:1426	arg1	levels					1408:1413	The levels	1404:1413	The levels of Cy3-siRNA in the ciliary muscle of the DMAPA-Glyp+siRNA group	1404:1478	The levels of Cy3-siRNA in the ciliary muscle of the DMAPA-Glyp+siRNA group did not significantly decrease over time.
33381584	7	52	theme	transfection	872:883	arg1	efficiency					885:894	Gene transfection efficiency	867:894	Gene transfection efficiency	867:894	Gene transfection efficiency and toxicity of the DMAPA-Glyp derivative were also evaluated.
33381584	12	53	from	IOP	1324:1326	arg1	group					1352:1356	the DMAPA-Glyp+siRNA group	1331:1356	the DMAPA-Glyp+siRNA group	1331:1356	IOP in the DMAPA-Glyp+siRNA group fell to the lowest level on day 3 after RNAi.
33381584	15	54	theme	IOP	1817:1819	arg1	values					1821:1826	the IOP values	1813:1826	the IOP values of rats	1813:1834	Taken together, our results suggest that downregulation of IκBα expression in the ciliary muscle plays a crucial role in reducing the IOP values of rats.
33381584	15	55	theme	IκBα	1742:1745	arg1	expression					1747:1756	IκBα expression	1742:1756	IκBα expression	1742:1756	Taken together, our results suggest that downregulation of IκBα expression in the ciliary muscle plays a crucial role in reducing the IOP values of rats.
33381584	3	56	theme	naked	547:551	arg1	complex					531:537	The Lipofectamine™ 2000 (Lipo)/siRNA complex	494:537	The Lipofectamine™ 2000 (Lipo)/siRNA complex	494:537	The Lipofectamine™ 2000 (Lipo)/siRNA complex and the naked siRNA were set as the controls.
33381584	3	56	theme	naked	547:551	arg1	controls					575:582	the controls	571:582	the controls	571:582	The Lipofectamine™ 2000 (Lipo)/siRNA complex and the naked siRNA were set as the controls.
33381584	3	56	theme	naked	547:551	arg1	siRNA					553:557	the naked siRNA	543:557	the naked siRNA	543:557	The Lipofectamine™ 2000 (Lipo)/siRNA complex and the naked siRNA were set as the controls.
33381584	1	57	theme	uveoscleral	174:184	arg1	pathway					194:200	the uveoscleral outflow pathway	170:200	the uveoscleral outflow pathway	170:200	The role of the IκB/NF-κB signaling pathway in the uveoscleral outflow pathway was investigated with IκBα gene silencing mediated by the 3-(dimethylamino)-1-propylamine-conjugated glycogen (DMAPA-Glyp) derivative.
33381584	17	58	theme	rat	1985:1987	arg1	eyes					1989:1992	rat eyes	1985:1992	rat eyes	1985:1992	The DMAPA-Glyp derivative is safe and feasible as an effective siRNA vector in rat eyes.
33381584	8	59	theme	protein	1004:1010	arg1	expression					1012:1021	protein expression	1004:1021	protein expression	1004:1021	After RNA interference (RNAi), IκBα mRNA and protein expression were significantly inhibited.
33381584	17	60	theme	DMAPA-Glyp	1910:1919	arg1	safe					1935:1938	safe	1935:1938	safe	1935:1938	The DMAPA-Glyp derivative is safe and feasible as an effective siRNA vector in rat eyes.
33381584	17	60	theme	DMAPA-Glyp	1910:1919	arg1	derivative					1921:1930	The DMAPA-Glyp derivative	1906:1930	The DMAPA-Glyp derivative	1906:1930	The DMAPA-Glyp derivative is safe and feasible as an effective siRNA vector in rat eyes.
33381584	0	61	from	Pathway	106:112	arg1	Rats					117:120	Rats	117:120	Rats	117:120	Hyperbranched Cationic Glycogen Derivative-Mediated IκBα Gene Silencing Regulates the Uveoscleral Outflow Pathway in Rats.
33381584	4	62	theme	NF-κBp65	626:633	arg1	protein					598:604	protein	598:604	protein	598:604	The mRNA and protein expression of IκBα, NF-κBp65, and MMP-2 were analyzed by real-time PCR, western blotting, and in situ gelatin zymography.
33381584	4	62	theme	NF-κBp65	626:633	arg1	mRNA					589:592	mRNA	589:592	mRNA	589:592	The mRNA and protein expression of IκBα, NF-κBp65, and MMP-2 were analyzed by real-time PCR, western blotting, and in situ gelatin zymography.
33381584	1	63	theme	IκBα	224:227	arg1	silencing					234:242	IκBα gene silencing	224:242	IκBα gene silencing mediated by the 3-(dimethylamino)-1-propylamine-conjugated glycogen (DMAPA-Glyp) derivative	224:334	The role of the IκB/NF-κB signaling pathway in the uveoscleral outflow pathway was investigated with IκBα gene silencing mediated by the 3-(dimethylamino)-1-propylamine-conjugated glycogen (DMAPA-Glyp) derivative.
33381584	17	64	from	vector	1975:1980	arg1	eyes					1989:1992	rat eyes	1985:1992	rat eyes	1985:1992	The DMAPA-Glyp derivative is safe and feasible as an effective siRNA vector in rat eyes.
33381584	12	65	from	level	1377:1381	arg1	day					1386:1388	day 3	1386:1390	day 3	1386:1390	IOP in the DMAPA-Glyp+siRNA group fell to the lowest level on day 3 after RNAi.
33381584	12	66	theme	DMAPA-Glyp+siRNA	1335:1350	arg1	group					1352:1356	the DMAPA-Glyp+siRNA group	1331:1356	the DMAPA-Glyp+siRNA group	1331:1356	IOP in the DMAPA-Glyp+siRNA group fell to the lowest level on day 3 after RNAi.
33381584	4	67	theme	in	700:701	arg1	zymography					716:725	in situ gelatin zymography	700:725	in situ gelatin zymography	700:725	The mRNA and protein expression of IκBα, NF-κBp65, and MMP-2 were analyzed by real-time PCR, western blotting, and in situ gelatin zymography.
33381584	15	68	theme	expression	1747:1756	arg1	downregulation					1724:1737	downregulation	1724:1737	downregulation of IκBα expression in the ciliary muscle	1724:1778	Taken together, our results suggest that downregulation of IκBα expression in the ciliary muscle plays a crucial role in reducing the IOP values of rats.
33381584	0	69	theme	Uveoscleral	86:96	arg1	Pathway					106:112	the Uveoscleral Outflow Pathway	82:112	the Uveoscleral Outflow Pathway in Rats	82:120	Hyperbranched Cationic Glycogen Derivative-Mediated IκBα Gene Silencing Regulates the Uveoscleral Outflow Pathway in Rats.
33381584	1	70	theme	signaling	149:157	arg1	pathway					159:165	the IκB/NF-κB signaling pathway	135:165	the IκB/NF-κB signaling pathway	135:165	The role of the IκB/NF-κB signaling pathway in the uveoscleral outflow pathway was investigated with IκBα gene silencing mediated by the 3-(dimethylamino)-1-propylamine-conjugated glycogen (DMAPA-Glyp) derivative.
33381584	14	71	theme	pathological	1563:1574	arg1	damage					1576:1581	no significant pathological damage	1548:1581	no significant pathological damage	1548:1581	At 7 and 14 d after RNAi, no significant pathological damage was detectable in the eyes injected with the DMAPA-Glyp derivative or the DMAPA-Glyp/siRNA complex.
33381584	5	72	theme	NF-κBp65	753:760	arg1	translocation					736:748	Nuclear translocation	728:748	Nuclear translocation of NF-κBp65	728:760	Nuclear translocation of NF-κBp65 was analyzed by immunofluorescence.
33381584	4	73	theme	western	678:684	arg1	blotting					686:693	western blotting	678:693	western blotting	678:693	The mRNA and protein expression of IκBα, NF-κBp65, and MMP-2 were analyzed by real-time PCR, western blotting, and in situ gelatin zymography.
33381584	9	74	theme	protein	1071:1077	arg1	expression					1079:1088	protein expression	1071:1088	protein expression	1071:1088	NF-κBp65 mRNA and protein expression showed no significant differences.
33381584	13	75	theme	DMAPA-Glyp+siRNA	1457:1472	arg1	group					1474:1478	the DMAPA-Glyp+siRNA group	1453:1478	the DMAPA-Glyp+siRNA group	1453:1478	The levels of Cy3-siRNA in the ciliary muscle of the DMAPA-Glyp+siRNA group did not significantly decrease over time.
33381584	2	76	theme	rats	422:425	arg1	muscles					411:417	the ciliary muscles	399:417	the ciliary muscles of rats	399:425	The IκBα-siRNA-loaded DMAPA-Glyp complex was transfected into the ciliary muscles of rats by intracameral injection (labeled as the DMAPA-Glyp+siRNA group).
33381584	0	77	theme	Cationic	14:21	arg1	Silencing					62:70	Hyperbranched Cationic Glycogen Derivative-Mediated IκBα Gene Silencing	0:70	Hyperbranched Cationic Glycogen Derivative-Mediated IκBα Gene Silencing	0:70	Hyperbranched Cationic Glycogen Derivative-Mediated IκBα Gene Silencing Regulates the Uveoscleral Outflow Pathway in Rats.
33381584	2	78	theme	DMAPA-Glyp+siRNA	469:484	arg1	group					486:490	the DMAPA-Glyp+siRNA group	465:490	the DMAPA-Glyp+siRNA group	465:490	The IκBα-siRNA-loaded DMAPA-Glyp complex was transfected into the ciliary muscles of rats by intracameral injection (labeled as the DMAPA-Glyp+siRNA group).
33381584	4	79	theme	gelatin	708:714	arg1	zymography					716:725	in situ gelatin zymography	700:725	in situ gelatin zymography	700:725	The mRNA and protein expression of IκBα, NF-κBp65, and MMP-2 were analyzed by real-time PCR, western blotting, and in situ gelatin zymography.
33381584	9	80	theme	significant	1100:1110	arg1	differences					1112:1122	no significant differences	1097:1122	no significant differences	1097:1122	NF-κBp65 mRNA and protein expression showed no significant differences.
33381584	0	81	theme	Derivative-Mediated	32:50	arg1	Silencing					62:70	Hyperbranched Cationic Glycogen Derivative-Mediated IκBα Gene Silencing	0:70	Hyperbranched Cationic Glycogen Derivative-Mediated IκBα Gene Silencing	0:70	Hyperbranched Cationic Glycogen Derivative-Mediated IκBα Gene Silencing Regulates the Uveoscleral Outflow Pathway in Rats.
33381584	1	82	theme	glycogen	303:310	arg1	derivative					325:334	the 3-(dimethylamino)-1-propylamine-conjugated glycogen (DMAPA-Glyp) derivative	256:334	the 3-(dimethylamino)-1-propylamine-conjugated glycogen (DMAPA-Glyp) derivative	256:334	The role of the IκB/NF-κB signaling pathway in the uveoscleral outflow pathway was investigated with IκBα gene silencing mediated by the 3-(dimethylamino)-1-propylamine-conjugated glycogen (DMAPA-Glyp) derivative.
33381584	11	83	theme	mRNA	1218:1221	arg1	expression					1223:1232	mRNA expression	1218:1232	mRNA expression	1218:1232	Both mRNA expression and activity of MMP-2 increased, with the largest increase in the DMAPA-Glyp+siRNA group.
33381584	3	84	theme	Lipofectamine™	498:511	arg1	complex					531:537	The Lipofectamine™ 2000 (Lipo)/siRNA complex	494:537	The Lipofectamine™ 2000 (Lipo)/siRNA complex	494:537	The Lipofectamine™ 2000 (Lipo)/siRNA complex and the naked siRNA were set as the controls.
33381584	3	84	theme	Lipofectamine™	498:511	arg1	controls					575:582	the controls	571:582	the controls	571:582	The Lipofectamine™ 2000 (Lipo)/siRNA complex and the naked siRNA were set as the controls.
33381584	3	84	theme	Lipofectamine™	498:511	arg1	siRNA					553:557	the naked siRNA	543:557	the naked siRNA	543:557	The Lipofectamine™ 2000 (Lipo)/siRNA complex and the naked siRNA were set as the controls.
33381584	0	85	theme	Gene	57:60	arg1	Silencing					62:70	Hyperbranched Cationic Glycogen Derivative-Mediated IκBα Gene Silencing	0:70	Hyperbranched Cationic Glycogen Derivative-Mediated IκBα Gene Silencing	0:70	Hyperbranched Cationic Glycogen Derivative-Mediated IκBα Gene Silencing Regulates the Uveoscleral Outflow Pathway in Rats.
33381584	1	86	theme	DMAPA-Glyp	313:322	arg1	derivative					325:334	the 3-(dimethylamino)-1-propylamine-conjugated glycogen (DMAPA-Glyp) derivative	256:334	the 3-(dimethylamino)-1-propylamine-conjugated glycogen (DMAPA-Glyp) derivative	256:334	The role of the IκB/NF-κB signaling pathway in the uveoscleral outflow pathway was investigated with IκBα gene silencing mediated by the 3-(dimethylamino)-1-propylamine-conjugated glycogen (DMAPA-Glyp) derivative.
33381584	11	87	theme	MMP-2	1250:1254	arg1	expression					1223:1232	mRNA expression	1218:1232	mRNA expression	1218:1232	Both mRNA expression and activity of MMP-2 increased, with the largest increase in the DMAPA-Glyp+siRNA group.
33381584	11	87	theme	MMP-2	1250:1254	arg1	activity					1238:1245	activity	1238:1245	activity of MMP-2	1238:1254	Both mRNA expression and activity of MMP-2 increased, with the largest increase in the DMAPA-Glyp+siRNA group.
33381584	14	88	from	detectable	1587:1596	arg1	eyes					1605:1608	the eyes	1601:1608	the eyes injected with the DMAPA-Glyp derivative or the DMAPA-Glyp/siRNA complex	1601:1680	At 7 and 14 d after RNAi, no significant pathological damage was detectable in the eyes injected with the DMAPA-Glyp derivative or the DMAPA-Glyp/siRNA complex.
33381584	2	89	theme	intracameral	430:441	arg1	injection					443:451	intracameral injection	430:451	intracameral injection (labeled as the DMAPA-Glyp+siRNA group)	430:491	The IκBα-siRNA-loaded DMAPA-Glyp complex was transfected into the ciliary muscles of rats by intracameral injection (labeled as the DMAPA-Glyp+siRNA group).
33381584	10	90	theme	NF-κBp65	1164:1171	arg1	translocation					1147:1159	nuclear translocation	1139:1159	nuclear translocation of NF-κBp65	1139:1171	Nevertheless, nuclear translocation of NF-κBp65 occurred in the DMAPA-Glyp+siRNA group.
33381584	2	91	theme	IκBα-siRNA-loaded	341:357	arg1	complex					370:376	The IκBα-siRNA-loaded DMAPA-Glyp complex	337:376	The IκBα-siRNA-loaded DMAPA-Glyp complex	337:376	The IκBα-siRNA-loaded DMAPA-Glyp complex was transfected into the ciliary muscles of rats by intracameral injection (labeled as the DMAPA-Glyp+siRNA group).
33381584	10	92	theme	DMAPA-Glyp+siRNA	1189:1204	arg1	group					1206:1210	the DMAPA-Glyp+siRNA group	1185:1210	the DMAPA-Glyp+siRNA group	1185:1210	Nevertheless, nuclear translocation of NF-κBp65 occurred in the DMAPA-Glyp+siRNA group.
33381584	11	93	from	increase	1284:1291	arg1	group					1317:1321	the DMAPA-Glyp+siRNA group	1296:1321	the DMAPA-Glyp+siRNA group	1296:1321	Both mRNA expression and activity of MMP-2 increased, with the largest increase in the DMAPA-Glyp+siRNA group.
33231621	15	0	theme	disease	1804:1810	arg1	severity					1812:1819	disease severity	1804:1819	disease severity	1804:1819	There was good correlation between other measures of visual function and disease severity.
33231621	15	1	theme	function	1791:1798	arg1	measures					1772:1779	other measures	1766:1779	other measures of visual function and disease severity	1766:1819	There was good correlation between other measures of visual function and disease severity.
33231621	13	2	theme	retinal	1518:1524	arg1	structure					1526:1534	retinal structure	1518:1534	retinal structure	1518:1534	Eyes with advanced disease based on retinal structure had significantly worse retinal function for several testing modalities.
33231621	10	3	theme	20-92	1181:1185	arg1	range					1174:1178	range	1174:1178	range	1174:1178	Median NEI-VFQ-39 and LLQ composite scores were 65 (range, 33-88) and 41 (range, 20-92), respectively.
33231621	6	4	theme	dark	850:853	arg1	adaptometry					855:865	dark adaptometry	850:865	dark adaptometry	850:865	Objective functional evaluations included Early Treatment of Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA), Pelli-Robson contrast sensitivity, mesopic microperimetry, and dark adaptometry.
33231621	7	5	theme	structural	916:925	arg1	category					935:942	structural disease category	916:942	structural disease category	916:942	Functional testing results were correlated with structural disease category.
33231621	11	6	theme	percent	1317:1323	arg1	thresholds					1333:1342	percent reduced thresholds	1317:1342	percent reduced thresholds	1317:1342	Median contrast sensitivity was 1.65 (range, 0.15-1.95), and median mesopic microperimetry average thresholds and percent reduced thresholds were 26 decibels (range, 0.4-28.6) and 21.6% (range, 0-100%), respectively.
33231621	13	7	theme	advanced	1492:1499	arg1	disease					1501:1507	advanced disease	1492:1507	advanced disease	1492:1507	Eyes with advanced disease based on retinal structure had significantly worse retinal function for several testing modalities.
33231621	1	8	theme	reading	200:206	arg1	symptoms					147:154	symptoms	147:154	symptoms of prolonged dark adaptation and difficulty reading	147:206	Purpose Individuals with pentosan polysulfate sodium (PPS) maculopathy commonly report symptoms of prolonged dark adaptation and difficulty reading.
33231621	11	9	dep	decibels	1352:1359	arg1	range					1362:1366	range	1362:1366	range	1362:1366	Median contrast sensitivity was 1.65 (range, 0.15-1.95), and median mesopic microperimetry average thresholds and percent reduced thresholds were 26 decibels (range, 0.4-28.6) and 21.6% (range, 0-100%), respectively.
33231621	14	10	theme	function	1664:1671	arg1	degradation					1673:1683	considerable visual function degradation	1644:1683	considerable visual function degradation that is not fully captured with BCVA testing	1644:1728	Conclusions PPS maculopathy causes considerable visual function degradation that is not fully captured with BCVA testing.
33231621	2	11	theme	acuity	314:319	arg1	testing					321:327	visual acuity testing	307:327	visual acuity testing	307:327	We hypothesize that PPS maculopathy causes degradation of visual function not fully captured with visual acuity testing.
33231621	6	12	theme	Retinopathy	724:734	arg1	BCVA					780:783	BCVA	780:783	BCVA	780:783	Objective functional evaluations included Early Treatment of Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA), Pelli-Robson contrast sensitivity, mesopic microperimetry, and dark adaptometry.
33231621	6	12	theme	Retinopathy	724:734	arg1	acuity					772:777	Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity	715:777	Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA)	715:784	Objective functional evaluations included Early Treatment of Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA), Pelli-Robson contrast sensitivity, mesopic microperimetry, and dark adaptometry.
33231621	14	13	theme	considerable	1644:1655	arg1	degradation					1673:1683	considerable visual function degradation	1644:1683	considerable visual function degradation that is not fully captured with BCVA testing	1644:1728	Conclusions PPS maculopathy causes considerable visual function degradation that is not fully captured with BCVA testing.
33231621	9	14	theme	ETDRS	1046:1050	arg1	score					1059:1063	Median ETDRS letter score	1039:1063	Median ETDRS letter score	1039:1063	Median ETDRS letter score was 82 (Snellen equivalent 20/25).
33231621	2	15	theme	PPS	229:231	arg1	maculopathy					233:243	PPS maculopathy	229:243	PPS maculopathy	229:243	We hypothesize that PPS maculopathy causes degradation of visual function not fully captured with visual acuity testing.
33231621	3	16	with	Subjects	338:345	arg1	maculopathy					356:366	PPS maculopathy	352:366	PPS maculopathy	352:366	Methods Subjects with PPS maculopathy underwent multimodal evaluation of retinal structure and function.
33231621	6	17	theme	Diabetic	715:722	arg1	Retinopathy					724:734	Diabetic Retinopathy	715:734	Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA)	715:784	Objective functional evaluations included Early Treatment of Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA), Pelli-Robson contrast sensitivity, mesopic microperimetry, and dark adaptometry.
33231621	14	18	theme	Conclusions	1609:1619	arg1	PPS					1621:1623	Conclusions PPS	1609:1623	Conclusions PPS maculopathy	1609:1635	Conclusions PPS maculopathy causes considerable visual function degradation that is not fully captured with BCVA testing.
33231621	2	19	theme	function	274:281	arg1	degradation					252:262	degradation	252:262	degradation of visual function not fully captured with visual acuity testing	252:327	We hypothesize that PPS maculopathy causes degradation of visual function not fully captured with visual acuity testing.
33231621	5	20	theme	Visual	569:574	arg1	Questionnaire					585:597	the National Eye Institute Visual Function Questionnaire 39	542:600	the National Eye Institute Visual Function Questionnaire 39 (NEI-VFQ-39)	542:613	Patient-reported visual function was assessed with the National Eye Institute Visual Function Questionnaire 39 (NEI-VFQ-39) and Low Luminance Questionnaire (LLQ).
33231621	5	20	theme	Visual	569:574	arg1	NEI-VFQ-39					603:612	NEI-VFQ-39	603:612	NEI-VFQ-39	603:612	Patient-reported visual function was assessed with the National Eye Institute Visual Function Questionnaire 39 (NEI-VFQ-39) and Low Luminance Questionnaire (LLQ).
33231621	12	21	theme	4.4-20.0	1471:1478	arg1	range					1464:1468	range	1464:1468	range	1464:1468	Median rod intercept time was 14.1 minutes (range, 4.4-20.0).
33231621	3	22	theme	PPS	352:354	arg1	maculopathy					356:366	PPS maculopathy	352:366	PPS maculopathy	352:366	Methods Subjects with PPS maculopathy underwent multimodal evaluation of retinal structure and function.
33231621	1	23	theme	dark	169:172	arg1	adaptation					174:183	prolonged dark adaptation	159:183	prolonged dark adaptation	159:183	Purpose Individuals with pentosan polysulfate sodium (PPS) maculopathy commonly report symptoms of prolonged dark adaptation and difficulty reading.
33231621	6	24	theme	functional	664:673	arg1	evaluations					675:685	Objective functional evaluations	654:685	Objective functional evaluations	654:685	Objective functional evaluations included Early Treatment of Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA), Pelli-Robson contrast sensitivity, mesopic microperimetry, and dark adaptometry.
33231621	12	25	dep	time	1441:1444	arg1	intercept					1431:1439	intercept	1431:1439	intercept	1431:1439	Median rod intercept time was 14.1 minutes (range, 4.4-20.0).
33231621	13	26	with	Eyes	1482:1485	arg1	disease					1501:1507	advanced disease	1492:1507	advanced disease	1492:1507	Eyes with advanced disease based on retinal structure had significantly worse retinal function for several testing modalities.
33231621	11	27	theme	mesopic	1271:1277	arg1	thresholds					1302:1311	median mesopic microperimetry average thresholds	1264:1311	median mesopic microperimetry average thresholds	1264:1311	Median contrast sensitivity was 1.65 (range, 0.15-1.95), and median mesopic microperimetry average thresholds and percent reduced thresholds were 26 decibels (range, 0.4-28.6) and 21.6% (range, 0-100%), respectively.
33231621	6	28	theme	acuity	772:777	arg1	Treatment					702:710	Early Treatment	696:710	Early Treatment of Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA), Pelli-Robson contrast sensitivity, mesopic microperimetry, and dark adaptometry	696:865	Objective functional evaluations included Early Treatment of Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA), Pelli-Robson contrast sensitivity, mesopic microperimetry, and dark adaptometry.
33231621	10	29	theme	LLQ	1122:1124	arg1	scores					1136:1141	Median NEI-VFQ-39 and LLQ composite scores	1100:1141	scores	1136:1141	Median NEI-VFQ-39 and LLQ composite scores were 65 (range, 33-88) and 41 (range, 20-92), respectively.
33231621	5	30	theme	Low	619:621	arg1	Questionnaire					633:645	Low Luminance Questionnaire	619:645	Low Luminance Questionnaire (LLQ)	619:651	Patient-reported visual function was assessed with the National Eye Institute Visual Function Questionnaire 39 (NEI-VFQ-39) and Low Luminance Questionnaire (LLQ).
33231621	5	30	theme	Low	619:621	arg1	LLQ					648:650	LLQ	648:650	LLQ	648:650	Patient-reported visual function was assessed with the National Eye Institute Visual Function Questionnaire 39 (NEI-VFQ-39) and Low Luminance Questionnaire (LLQ).
33231621	1	31	theme	pentosan	85:92	arg1	polysulfate					94:104	pentosan polysulfate	85:104	pentosan polysulfate sodium (PPS) maculopathy	85:129	Purpose Individuals with pentosan polysulfate sodium (PPS) maculopathy commonly report symptoms of prolonged dark adaptation and difficulty reading.
33231621	6	32	theme	best-corrected	750:763	arg1	BCVA					780:783	BCVA	780:783	BCVA	780:783	Objective functional evaluations included Early Treatment of Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA), Pelli-Robson contrast sensitivity, mesopic microperimetry, and dark adaptometry.
33231621	6	32	theme	best-corrected	750:763	arg1	acuity					772:777	Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity	715:777	Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA)	715:784	Objective functional evaluations included Early Treatment of Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA), Pelli-Robson contrast sensitivity, mesopic microperimetry, and dark adaptometry.
33231621	0	33	theme	Visual	0:5	arg1	Function					7:14	Visual Function	0:14	Visual Function in Pentosan Polysulfate Sodium Maculopathy.	0:58	Visual Function in Pentosan Polysulfate Sodium Maculopathy.
33231621	7	34	theme	Functional	868:877	arg1	results					887:893	Functional testing results	868:893	Functional testing results	868:893	Functional testing results were correlated with structural disease category.
33231621	1	35	theme	sodium	106:111	arg1	maculopathy					119:129	pentosan polysulfate sodium (PPS) maculopathy	85:129	pentosan polysulfate sodium (PPS) maculopathy	85:129	Purpose Individuals with pentosan polysulfate sodium (PPS) maculopathy commonly report symptoms of prolonged dark adaptation and difficulty reading.
33231621	3	36	theme	retinal	403:409	arg1	structure					411:419	retinal structure	403:419	retinal structure	403:419	Methods Subjects with PPS maculopathy underwent multimodal evaluation of retinal structure and function.
33231621	11	37	theme	contrast	1210:1217	arg1	sensitivity					1219:1229	Median contrast sensitivity	1203:1229	Median contrast sensitivity	1203:1229	Median contrast sensitivity was 1.65 (range, 0.15-1.95), and median mesopic microperimetry average thresholds and percent reduced thresholds were 26 decibels (range, 0.4-28.6) and 21.6% (range, 0-100%), respectively.
33231621	6	38	theme	ETDRS	743:747	arg1	BCVA					780:783	BCVA	780:783	BCVA	780:783	Objective functional evaluations included Early Treatment of Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA), Pelli-Robson contrast sensitivity, mesopic microperimetry, and dark adaptometry.
33231621	6	38	theme	ETDRS	743:747	arg1	acuity					772:777	Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity	715:777	Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA)	715:784	Objective functional evaluations included Early Treatment of Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA), Pelli-Robson contrast sensitivity, mesopic microperimetry, and dark adaptometry.
33231621	13	39	theme	testing	1589:1595	arg1	modalities					1597:1606	several testing modalities	1581:1606	several testing modalities	1581:1606	Eyes with advanced disease based on retinal structure had significantly worse retinal function for several testing modalities.
33231621	6	40	theme	Study	736:740	arg1	BCVA					780:783	BCVA	780:783	BCVA	780:783	Objective functional evaluations included Early Treatment of Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA), Pelli-Robson contrast sensitivity, mesopic microperimetry, and dark adaptometry.
33231621	6	40	theme	Study	736:740	arg1	acuity					772:777	Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity	715:777	Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA)	715:784	Objective functional evaluations included Early Treatment of Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA), Pelli-Robson contrast sensitivity, mesopic microperimetry, and dark adaptometry.
33231621	0	41	theme	Polysulfate	28:38	arg1	Maculopathy					47:57	Pentosan Polysulfate Sodium Maculopathy	19:57	Pentosan Polysulfate Sodium Maculopathy	19:57	Visual Function in Pentosan Polysulfate Sodium Maculopathy.
33231621	16	42	theme	safety	1879:1884	arg1	issue					1886:1890	this patient safety issue	1866:1890	this patient safety issue	1866:1890	These findings deepen our concern regarding this patient safety issue.
33231621	8	43	theme	age	989:991	arg1	Results					945:951	Results Thirteen patients (26 eyes), median age 62 years (range, 37-76),	945:1016	Results	945:951	Results Thirteen patients (26 eyes), median age 62 years (range, 37-76), completed the study.
33231621	8	43	theme	age	989:991	arg1	years					996:1000	median age 62 years	982:1000	median age 62 years (range, 37-76)	982:1015	Results Thirteen patients (26 eyes), median age 62 years (range, 37-76), completed the study.
33231621	12	44	dep	minutes	1455:1461	arg1	range					1464:1468	range	1464:1468	range	1464:1468	Median rod intercept time was 14.1 minutes (range, 4.4-20.0).
33231621	13	45	theme	retinal	1560:1566	arg1	function					1568:1575	worse retinal function	1554:1575	worse retinal function for several testing modalities	1554:1606	Eyes with advanced disease based on retinal structure had significantly worse retinal function for several testing modalities.
33231621	6	46	theme	contrast	800:807	arg1	sensitivity					809:819	Pelli-Robson contrast sensitivity	787:819	Pelli-Robson contrast sensitivity	787:819	Objective functional evaluations included Early Treatment of Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA), Pelli-Robson contrast sensitivity, mesopic microperimetry, and dark adaptometry.
33231621	14	47	theme	BCVA	1717:1720	arg1	testing					1722:1728	BCVA testing	1717:1728	BCVA testing	1717:1728	Conclusions PPS maculopathy causes considerable visual function degradation that is not fully captured with BCVA testing.
33231621	12	48	theme	rod	1427:1429	arg1	time					1441:1444	Median rod intercept time	1420:1444	Median rod intercept time	1420:1444	Median rod intercept time was 14.1 minutes (range, 4.4-20.0).
33231621	5	49	theme	Eye	555:557	arg1	Institute					559:567	the National Eye Institute	542:567	the National Eye Institute Visual Function Questionnaire 39 (NEI-VFQ-39)	542:613	Patient-reported visual function was assessed with the National Eye Institute Visual Function Questionnaire 39 (NEI-VFQ-39) and Low Luminance Questionnaire (LLQ).
33231621	15	50	theme	severity	1812:1819	arg1	measures					1772:1779	other measures	1766:1779	other measures of visual function and disease severity	1766:1819	There was good correlation between other measures of visual function and disease severity.
33231621	6	51	theme	Early	696:700	arg1	Treatment					702:710	Early Treatment	696:710	Early Treatment of Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA), Pelli-Robson contrast sensitivity, mesopic microperimetry, and dark adaptometry	696:865	Objective functional evaluations included Early Treatment of Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA), Pelli-Robson contrast sensitivity, mesopic microperimetry, and dark adaptometry.
33231621	5	52	theme	visual	508:513	arg1	function					515:522	Patient-reported visual function	491:522	Patient-reported visual function	491:522	Patient-reported visual function was assessed with the National Eye Institute Visual Function Questionnaire 39 (NEI-VFQ-39) and Low Luminance Questionnaire (LLQ).
33231621	8	53	theme	37-76	1010:1014	arg1	range					1003:1007	range	1003:1007	range	1003:1007	Results Thirteen patients (26 eyes), median age 62 years (range, 37-76), completed the study.
33231621	4	54	theme	Structural	435:444	arg1	changes					446:452	Structural changes	435:452	Structural changes	435:452	Structural changes were graded as moderate or advanced.
33231621	15	55	theme	visual	1784:1789	arg1	function					1791:1798	visual function	1784:1798	visual function	1784:1798	There was good correlation between other measures of visual function and disease severity.
33231621	2	56	theme	visual	307:312	arg1	testing					321:327	visual acuity testing	307:327	visual acuity testing	307:327	We hypothesize that PPS maculopathy causes degradation of visual function not fully captured with visual acuity testing.
33231621	7	57	theme	disease	927:933	arg1	category					935:942	structural disease category	916:942	structural disease category	916:942	Functional testing results were correlated with structural disease category.
33231621	11	58	theme	0.4-28.6	1369:1376	arg1	range					1362:1366	range	1362:1366	range	1362:1366	Median contrast sensitivity was 1.65 (range, 0.15-1.95), and median mesopic microperimetry average thresholds and percent reduced thresholds were 26 decibels (range, 0.4-28.6) and 21.6% (range, 0-100%), respectively.
33231621	6	59	theme	adaptometry	855:865	arg1	Treatment					702:710	Early Treatment	696:710	Early Treatment of Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA), Pelli-Robson contrast sensitivity, mesopic microperimetry, and dark adaptometry	696:865	Objective functional evaluations included Early Treatment of Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA), Pelli-Robson contrast sensitivity, mesopic microperimetry, and dark adaptometry.
33231621	5	60	theme	National	546:553	arg1	Institute					559:567	the National Eye Institute	542:567	the National Eye Institute Visual Function Questionnaire 39 (NEI-VFQ-39)	542:613	Patient-reported visual function was assessed with the National Eye Institute Visual Function Questionnaire 39 (NEI-VFQ-39) and Low Luminance Questionnaire (LLQ).
33231621	1	61	theme	adaptation	174:183	arg1	symptoms					147:154	symptoms	147:154	symptoms of prolonged dark adaptation and difficulty reading	147:206	Purpose Individuals with pentosan polysulfate sodium (PPS) maculopathy commonly report symptoms of prolonged dark adaptation and difficulty reading.
33231621	6	62	theme	Objective	654:662	arg1	evaluations					675:685	Objective functional evaluations	654:685	Objective functional evaluations	654:685	Objective functional evaluations included Early Treatment of Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA), Pelli-Robson contrast sensitivity, mesopic microperimetry, and dark adaptometry.
33231621	5	63	theme	Institute	559:567	arg1	Questionnaire					585:597	the National Eye Institute Visual Function Questionnaire 39	542:600	the National Eye Institute Visual Function Questionnaire 39 (NEI-VFQ-39)	542:613	Patient-reported visual function was assessed with the National Eye Institute Visual Function Questionnaire 39 (NEI-VFQ-39) and Low Luminance Questionnaire (LLQ).
33231621	5	63	theme	Institute	559:567	arg1	NEI-VFQ-39					603:612	NEI-VFQ-39	603:612	NEI-VFQ-39	603:612	Patient-reported visual function was assessed with the National Eye Institute Visual Function Questionnaire 39 (NEI-VFQ-39) and Low Luminance Questionnaire (LLQ).
33231621	1	64	theme	difficulty	189:198	arg1	reading					200:206	difficulty reading	189:206	difficulty reading	189:206	Purpose Individuals with pentosan polysulfate sodium (PPS) maculopathy commonly report symptoms of prolonged dark adaptation and difficulty reading.
33231621	11	65	theme	average	1294:1300	arg1	thresholds					1302:1311	median mesopic microperimetry average thresholds	1264:1311	median mesopic microperimetry average thresholds	1264:1311	Median contrast sensitivity was 1.65 (range, 0.15-1.95), and median mesopic microperimetry average thresholds and percent reduced thresholds were 26 decibels (range, 0.4-28.6) and 21.6% (range, 0-100%), respectively.
33231621	6	66	theme	microperimetry	830:843	arg1	Treatment					702:710	Early Treatment	696:710	Early Treatment of Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA), Pelli-Robson contrast sensitivity, mesopic microperimetry, and dark adaptometry	696:865	Objective functional evaluations included Early Treatment of Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA), Pelli-Robson contrast sensitivity, mesopic microperimetry, and dark adaptometry.
33231621	14	67	theme	visual	1657:1662	arg1	degradation					1673:1683	considerable visual function degradation	1644:1683	considerable visual function degradation that is not fully captured with BCVA testing	1644:1728	Conclusions PPS maculopathy causes considerable visual function degradation that is not fully captured with BCVA testing.
33231621	16	68	theme	patient	1871:1877	arg1	issue					1886:1890	this patient safety issue	1866:1890	this patient safety issue	1866:1890	These findings deepen our concern regarding this patient safety issue.
33231621	11	69	dep	%	1387:1387	arg1	range					1390:1394	range	1390:1394	range	1390:1394	Median contrast sensitivity was 1.65 (range, 0.15-1.95), and median mesopic microperimetry average thresholds and percent reduced thresholds were 26 decibels (range, 0.4-28.6) and 21.6% (range, 0-100%), respectively.
33231621	11	69	dep	%	1387:1387	arg1	%					1402:1402	0-100%	1397:1402	0-100%	1397:1402	Median contrast sensitivity was 1.65 (range, 0.15-1.95), and median mesopic microperimetry average thresholds and percent reduced thresholds were 26 decibels (range, 0.4-28.6) and 21.6% (range, 0-100%), respectively.
33231621	11	70	theme	reduced	1325:1331	arg1	thresholds					1333:1342	percent reduced thresholds	1317:1342	percent reduced thresholds	1317:1342	Median contrast sensitivity was 1.65 (range, 0.15-1.95), and median mesopic microperimetry average thresholds and percent reduced thresholds were 26 decibels (range, 0.4-28.6) and 21.6% (range, 0-100%), respectively.
33231621	1	71	with	Individuals	68:78	arg1	maculopathy					119:129	pentosan polysulfate sodium (PPS) maculopathy	85:129	pentosan polysulfate sodium (PPS) maculopathy	85:129	Purpose Individuals with pentosan polysulfate sodium (PPS) maculopathy commonly report symptoms of prolonged dark adaptation and difficulty reading.
33231621	3	72	theme	Methods	330:336	arg1	Subjects					338:345	Methods Subjects	330:345	Methods Subjects with PPS maculopathy	330:366	Methods Subjects with PPS maculopathy underwent multimodal evaluation of retinal structure and function.
33231621	9	73	theme	letter	1052:1057	arg1	score					1059:1063	Median ETDRS letter score	1039:1063	Median ETDRS letter score	1039:1063	Median ETDRS letter score was 82 (Snellen equivalent 20/25).
33231621	8	74	dep	Results	945:951	arg1	patients					962:969	Thirteen patients	953:969	Thirteen patients (26 eyes)	953:979	Results Thirteen patients (26 eyes), median age 62 years (range, 37-76), completed the study.
33231621	8	74	dep	Results	945:951	arg1	eyes					975:978	26 eyes	972:978	26 eyes	972:978	Results Thirteen patients (26 eyes), median age 62 years (range, 37-76), completed the study.
33231621	14	75	theme	PPS	1621:1623	arg1	maculopathy					1625:1635	Conclusions PPS maculopathy	1609:1635	Conclusions PPS maculopathy	1609:1635	Conclusions PPS maculopathy causes considerable visual function degradation that is not fully captured with BCVA testing.
33231621	2	76	theme	visual	267:272	arg1	function					274:281	visual function	267:281	visual function not fully captured with visual acuity testing	267:327	We hypothesize that PPS maculopathy causes degradation of visual function not fully captured with visual acuity testing.
33231621	5	77	theme	Function	576:583	arg1	Questionnaire					585:597	the National Eye Institute Visual Function Questionnaire 39	542:600	the National Eye Institute Visual Function Questionnaire 39 (NEI-VFQ-39)	542:613	Patient-reported visual function was assessed with the National Eye Institute Visual Function Questionnaire 39 (NEI-VFQ-39) and Low Luminance Questionnaire (LLQ).
33231621	5	77	theme	Function	576:583	arg1	NEI-VFQ-39					603:612	NEI-VFQ-39	603:612	NEI-VFQ-39	603:612	Patient-reported visual function was assessed with the National Eye Institute Visual Function Questionnaire 39 (NEI-VFQ-39) and Low Luminance Questionnaire (LLQ).
33231621	8	78	dep	years	996:1000	arg1	range					1003:1007	range	1003:1007	range	1003:1007	Results Thirteen patients (26 eyes), median age 62 years (range, 37-76), completed the study.
33231621	10	79	theme	Median	1100:1105	arg1	NEI-VFQ-39					1107:1116	Median NEI-VFQ-39 and LLQ composite scores	1100:1141	NEI-VFQ-39	1107:1116	Median NEI-VFQ-39 and LLQ composite scores were 65 (range, 33-88) and 41 (range, 20-92), respectively.
33231621	1	80	theme	prolonged	159:167	arg1	adaptation					174:183	prolonged dark adaptation	159:183	prolonged dark adaptation	159:183	Purpose Individuals with pentosan polysulfate sodium (PPS) maculopathy commonly report symptoms of prolonged dark adaptation and difficulty reading.
33231621	0	81	from	Function	7:14	arg1	Maculopathy					47:57	Pentosan Polysulfate Sodium Maculopathy	19:57	Pentosan Polysulfate Sodium Maculopathy	19:57	Visual Function in Pentosan Polysulfate Sodium Maculopathy.
33231621	9	82	theme	equivalent	1081:1090	arg1	Snellen					1073:1079	Snellen equivalent 20/25	1073:1096	Snellen equivalent 20/25	1073:1096	Median ETDRS letter score was 82 (Snellen equivalent 20/25).
33231621	9	82	theme	equivalent	1081:1090	arg1	82					1069:1070	82	1069:1070	82	1069:1070	Median ETDRS letter score was 82 (Snellen equivalent 20/25).
33231621	1	83	theme	Purpose	60:66	arg1	Individuals					68:78	Purpose Individuals	60:78	Purpose Individuals with pentosan polysulfate sodium (PPS) maculopathy	60:129	Purpose Individuals with pentosan polysulfate sodium (PPS) maculopathy commonly report symptoms of prolonged dark adaptation and difficulty reading.
33231621	10	84	theme	composite	1126:1134	arg1	scores					1136:1141	Median NEI-VFQ-39 and LLQ composite scores	1100:1141	scores	1136:1141	Median NEI-VFQ-39 and LLQ composite scores were 65 (range, 33-88) and 41 (range, 20-92), respectively.
33231621	3	85	theme	multimodal	378:387	arg1	evaluation					389:398	multimodal evaluation	378:398	multimodal evaluation of retinal structure and function	378:432	Methods Subjects with PPS maculopathy underwent multimodal evaluation of retinal structure and function.
33231621	11	86	theme	microperimetry	1279:1292	arg1	thresholds					1302:1311	median mesopic microperimetry average thresholds	1264:1311	median mesopic microperimetry average thresholds	1264:1311	Median contrast sensitivity was 1.65 (range, 0.15-1.95), and median mesopic microperimetry average thresholds and percent reduced thresholds were 26 decibels (range, 0.4-28.6) and 21.6% (range, 0-100%), respectively.
33231621	6	87	theme	visual	765:770	arg1	BCVA					780:783	BCVA	780:783	BCVA	780:783	Objective functional evaluations included Early Treatment of Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA), Pelli-Robson contrast sensitivity, mesopic microperimetry, and dark adaptometry.
33231621	6	87	theme	visual	765:770	arg1	acuity					772:777	Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity	715:777	Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA)	715:784	Objective functional evaluations included Early Treatment of Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA), Pelli-Robson contrast sensitivity, mesopic microperimetry, and dark adaptometry.
33231621	13	88	contain	had	1536:1538	arg2	function					1568:1575	worse retinal function	1554:1575	worse retinal function for several testing modalities	1554:1606	Eyes with advanced disease based on retinal structure had significantly worse retinal function for several testing modalities.
33231621	13	88	contain	had	1536:1538	arg1	Eyes					1482:1485	Eyes	1482:1485	Eyes with advanced disease based on retinal structure	1482:1534	Eyes with advanced disease based on retinal structure had significantly worse retinal function for several testing modalities.
33231621	5	89	theme	Luminance	623:631	arg1	Questionnaire					633:645	Low Luminance Questionnaire	619:645	Low Luminance Questionnaire (LLQ)	619:651	Patient-reported visual function was assessed with the National Eye Institute Visual Function Questionnaire 39 (NEI-VFQ-39) and Low Luminance Questionnaire (LLQ).
33231621	5	89	theme	Luminance	623:631	arg1	LLQ					648:650	LLQ	648:650	LLQ	648:650	Patient-reported visual function was assessed with the National Eye Institute Visual Function Questionnaire 39 (NEI-VFQ-39) and Low Luminance Questionnaire (LLQ).
33231621	1	90	theme	polysulfate	94:104	arg1	sodium					106:111	pentosan polysulfate sodium	85:111	pentosan polysulfate sodium (PPS) maculopathy	85:129	Purpose Individuals with pentosan polysulfate sodium (PPS) maculopathy commonly report symptoms of prolonged dark adaptation and difficulty reading.
33231621	1	90	theme	polysulfate	94:104	arg1	PPS					114:116	PPS	114:116	PPS	114:116	Purpose Individuals with pentosan polysulfate sodium (PPS) maculopathy commonly report symptoms of prolonged dark adaptation and difficulty reading.
33231621	3	91	theme	function	425:432	arg1	evaluation					389:398	multimodal evaluation	378:398	multimodal evaluation of retinal structure and function	378:432	Methods Subjects with PPS maculopathy underwent multimodal evaluation of retinal structure and function.
33231621	11	92	theme	median	1264:1269	arg1	thresholds					1302:1311	median mesopic microperimetry average thresholds	1264:1311	median mesopic microperimetry average thresholds	1264:1311	Median contrast sensitivity was 1.65 (range, 0.15-1.95), and median mesopic microperimetry average thresholds and percent reduced thresholds were 26 decibels (range, 0.4-28.6) and 21.6% (range, 0-100%), respectively.
33231621	0	93	theme	Pentosan	19:26	arg1	Polysulfate					28:38	Pentosan Polysulfate	19:38	Pentosan Polysulfate Sodium Maculopathy	19:57	Visual Function in Pentosan Polysulfate Sodium Maculopathy.
33231621	7	94	theme	testing	879:885	arg1	results					887:893	Functional testing results	868:893	Functional testing results	868:893	Functional testing results were correlated with structural disease category.
33231621	3	95	theme	structure	411:419	arg1	evaluation					389:398	multimodal evaluation	378:398	multimodal evaluation of retinal structure and function	378:432	Methods Subjects with PPS maculopathy underwent multimodal evaluation of retinal structure and function.
33231621	13	96	theme	several	1581:1587	arg1	modalities					1597:1606	several testing modalities	1581:1606	several testing modalities	1581:1606	Eyes with advanced disease based on retinal structure had significantly worse retinal function for several testing modalities.
33231621	0	97	theme	Sodium	40:45	arg1	Maculopathy					47:57	Pentosan Polysulfate Sodium Maculopathy	19:57	Pentosan Polysulfate Sodium Maculopathy	19:57	Visual Function in Pentosan Polysulfate Sodium Maculopathy.
33231621	15	98	theme	other	1766:1770	arg1	measures					1772:1779	other measures	1766:1779	other measures of visual function and disease severity	1766:1819	There was good correlation between other measures of visual function and disease severity.
33231621	11	99	theme	Median	1203:1208	arg1	sensitivity					1219:1229	Median contrast sensitivity	1203:1229	Median contrast sensitivity	1203:1229	Median contrast sensitivity was 1.65 (range, 0.15-1.95), and median mesopic microperimetry average thresholds and percent reduced thresholds were 26 decibels (range, 0.4-28.6) and 21.6% (range, 0-100%), respectively.
33231621	6	100	theme	mesopic	822:828	arg1	microperimetry					830:843	mesopic microperimetry	822:843	mesopic microperimetry	822:843	Objective functional evaluations included Early Treatment of Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA), Pelli-Robson contrast sensitivity, mesopic microperimetry, and dark adaptometry.
33231621	8	101	theme	median	982:987	arg1	Results					945:951	Results Thirteen patients (26 eyes), median age 62 years (range, 37-76),	945:1016	Results	945:951	Results Thirteen patients (26 eyes), median age 62 years (range, 37-76), completed the study.
33231621	8	101	theme	median	982:987	arg1	years					996:1000	median age 62 years	982:1000	median age 62 years (range, 37-76)	982:1015	Results Thirteen patients (26 eyes), median age 62 years (range, 37-76), completed the study.
33231621	13	102	theme	worse	1554:1558	arg1	function					1568:1575	worse retinal function	1554:1575	worse retinal function for several testing modalities	1554:1606	Eyes with advanced disease based on retinal structure had significantly worse retinal function for several testing modalities.
33231621	6	103	theme	sensitivity	809:819	arg1	Treatment					702:710	Early Treatment	696:710	Early Treatment of Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA), Pelli-Robson contrast sensitivity, mesopic microperimetry, and dark adaptometry	696:865	Objective functional evaluations included Early Treatment of Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA), Pelli-Robson contrast sensitivity, mesopic microperimetry, and dark adaptometry.
33231621	10	104	dep	41	1170:1171	arg1	range					1174:1178	range	1174:1178	range	1174:1178	Median NEI-VFQ-39 and LLQ composite scores were 65 (range, 33-88) and 41 (range, 20-92), respectively.
33231621	6	105	theme	Pelli-Robson	787:798	arg1	sensitivity					809:819	Pelli-Robson contrast sensitivity	787:819	Pelli-Robson contrast sensitivity	787:819	Objective functional evaluations included Early Treatment of Diabetic Retinopathy Study (ETDRS) best-corrected visual acuity (BCVA), Pelli-Robson contrast sensitivity, mesopic microperimetry, and dark adaptometry.
33231621	5	106	theme	Patient-reported	491:506	arg1	function					515:522	Patient-reported visual function	491:522	Patient-reported visual function	491:522	Patient-reported visual function was assessed with the National Eye Institute Visual Function Questionnaire 39 (NEI-VFQ-39) and Low Luminance Questionnaire (LLQ).
33231621	15	107	theme	good	1741:1744	arg1	correlation					1746:1756	good correlation	1741:1756	good correlation between other measures of visual function and disease severity	1741:1819	There was good correlation between other measures of visual function and disease severity.
33231621	12	108	theme	Median	1420:1425	arg1	time					1441:1444	Median rod intercept time	1420:1444	Median rod intercept time	1420:1444	Median rod intercept time was 14.1 minutes (range, 4.4-20.0).
33231621	9	109	theme	Median	1039:1044	arg1	score					1059:1063	Median ETDRS letter score	1039:1063	Median ETDRS letter score	1039:1063	Median ETDRS letter score was 82 (Snellen equivalent 20/25).
34581895	1	0	with	variability	237:247	arg1	regards					254:260	regards	254:260	regards to clinical outcomes and treatment response	254:304	Prostate Cancer (PC) is a disease with remarkable tumor heterogeneity that often manifests in significant intra-patient variability with regards to clinical outcomes and treatment response.
34581895	6	1	theme	novel	1332:1336	arg1	protocol					1372:1379	a novel microfluidic rare-event screening protocol	1330:1379	a novel microfluidic rare-event screening protocol	1330:1379	Furthermore, to confirm the tumor-origin of the PDCOs we have analyzed the expression of PC-associated epigenetic biomarkers including promoter methylation of the GSTP1, RASSF1 and APC and RARb genes by employing a novel microfluidic rare-event screening protocol.
34581895	3	2	theme	current	520:526	arg1	study					528:532	current study	520:532	current study	520:532	In current study, we established ex vivo primary patient-derived cancer organoid (PDCO) cultures from prostatectomy specimens of patients with locally advanced PC.
34581895	1	3	theme	clinical	265:272	arg1	outcomes					274:281	clinical outcomes	265:281	clinical outcomes	265:281	Prostate Cancer (PC) is a disease with remarkable tumor heterogeneity that often manifests in significant intra-patient variability with regards to clinical outcomes and treatment response.
34581895	7	4	theme	multifocal	1510:1519	arg1	PC					1521:1522	multifocal PC	1510:1522	multifocal PC using orthogonal analyses	1510:1548	These results demonstrate that this ex vivo PDCO model recapitulates the complexity of the epithelial tumor microenvironment of multifocal PC using orthogonal analyses.
34581895	6	5	theme	biomarkers	1231:1240	arg1	expression					1192:1201	the expression	1188:1201	the expression of PC-associated epigenetic biomarkers including promoter methylation of the GSTP1, RASSF1 and APC and RARb genes	1188:1315	Furthermore, to confirm the tumor-origin of the PDCOs we have analyzed the expression of PC-associated epigenetic biomarkers including promoter methylation of the GSTP1, RASSF1 and APC and RARb genes by employing a novel microfluidic rare-event screening protocol.
34581895	6	6	theme	genes	1311:1315	arg1	methylation					1261:1271	promoter methylation	1252:1271	promoter methylation of the GSTP1, RASSF1 and APC and RARb genes	1252:1315	Furthermore, to confirm the tumor-origin of the PDCOs we have analyzed the expression of PC-associated epigenetic biomarkers including promoter methylation of the GSTP1, RASSF1 and APC and RARb genes by employing a novel microfluidic rare-event screening protocol.
34581895	1	7	theme	tumor	167:171	arg1	heterogeneity					173:185	remarkable tumor heterogeneity	156:185	remarkable tumor heterogeneity	156:185	Prostate Cancer (PC) is a disease with remarkable tumor heterogeneity that often manifests in significant intra-patient variability with regards to clinical outcomes and treatment response.
34581895	7	8	theme	orthogonal	1530:1539	arg1	analyses					1541:1548	orthogonal analyses	1530:1548	orthogonal analyses	1530:1548	These results demonstrate that this ex vivo PDCO model recapitulates the complexity of the epithelial tumor microenvironment of multifocal PC using orthogonal analyses.
34581895	6	9	theme	PC-associated	1206:1218	arg1	biomarkers					1231:1240	PC-associated epigenetic biomarkers	1206:1240	PC-associated epigenetic biomarkers including promoter methylation of the GSTP1, RASSF1 and APC and RARb genes	1206:1315	Furthermore, to confirm the tumor-origin of the PDCOs we have analyzed the expression of PC-associated epigenetic biomarkers including promoter methylation of the GSTP1, RASSF1 and APC and RARb genes by employing a novel microfluidic rare-event screening protocol.
34581895	6	9	theme	PC-associated	1206:1218	arg1	methylation					1261:1271	promoter methylation	1252:1271	promoter methylation of the GSTP1, RASSF1 and APC and RARb genes	1252:1315	Furthermore, to confirm the tumor-origin of the PDCOs we have analyzed the expression of PC-associated epigenetic biomarkers including promoter methylation of the GSTP1, RASSF1 and APC and RARb genes by employing a novel microfluidic rare-event screening protocol.
34581895	2	10	theme	cell	329:332	arg1	lines					334:338	Commonly available PC cell lines	307:338	Commonly available PC cell lines	307:338	Commonly available PC cell lines do not accurately reflect the complexity of this disease and there is critical need for development of new models to recapitulate the intricate hierarchy of tumor pathogenesis.
34581895	3	11	with	patients	646:653	arg1	PC					677:678	locally advanced PC	660:678	locally advanced PC	660:678	In current study, we established ex vivo primary patient-derived cancer organoid (PDCO) cultures from prostatectomy specimens of patients with locally advanced PC.
34581895	2	12	theme	available	316:324	arg1	lines					334:338	Commonly available PC cell lines	307:338	Commonly available PC cell lines	307:338	Commonly available PC cell lines do not accurately reflect the complexity of this disease and there is critical need for development of new models to recapitulate the intricate hierarchy of tumor pathogenesis.
34581895	7	13	dep	ex	1418:1419	arg1	vivo					1421:1424	vivo	1421:1424	vivo	1421:1424	These results demonstrate that this ex vivo PDCO model recapitulates the complexity of the epithelial tumor microenvironment of multifocal PC using orthogonal analyses.
34581895	6	14	theme	APC	1298:1300	arg1	genes					1311:1315	the GSTP1, RASSF1 and APC and RARb genes	1276:1315	the GSTP1, RASSF1 and APC and RARb genes	1276:1315	Furthermore, to confirm the tumor-origin of the PDCOs we have analyzed the expression of PC-associated epigenetic biomarkers including promoter methylation of the GSTP1, RASSF1 and APC and RARb genes by employing a novel microfluidic rare-event screening protocol.
34581895	5	15	theme	orthogonal	897:906	arg1	analysis					923:930	orthogonal flow cytometry analysis	897:930	orthogonal flow cytometry analysis	897:930	Using orthogonal flow cytometry analysis, we demonstrate that primary PDCOs maintain distinct subsets of epithelial cells throughout culture and that these cells conserve expression of androgen receptor (AR)-related elements.
34581895	6	16	theme	RASSF1	1287:1292	arg1	genes					1311:1315	the GSTP1, RASSF1 and APC and RARb genes	1276:1315	the GSTP1, RASSF1 and APC and RARb genes	1276:1315	Furthermore, to confirm the tumor-origin of the PDCOs we have analyzed the expression of PC-associated epigenetic biomarkers including promoter methylation of the GSTP1, RASSF1 and APC and RARb genes by employing a novel microfluidic rare-event screening protocol.
34581895	3	17	theme	PDCO	599:602	arg1	cultures					605:612	ex vivo primary patient-derived cancer organoid (PDCO) cultures	550:612	ex vivo primary patient-derived cancer organoid (PDCO) cultures from prostatectomy specimens of patients with locally advanced PC	550:678	In current study, we established ex vivo primary patient-derived cancer organoid (PDCO) cultures from prostatectomy specimens of patients with locally advanced PC.
34581895	6	18	theme	GSTP1	1280:1284	arg1	genes					1311:1315	the GSTP1, RASSF1 and APC and RARb genes	1276:1315	the GSTP1, RASSF1 and APC and RARb genes	1276:1315	Furthermore, to confirm the tumor-origin of the PDCOs we have analyzed the expression of PC-associated epigenetic biomarkers including promoter methylation of the GSTP1, RASSF1 and APC and RARb genes by employing a novel microfluidic rare-event screening protocol.
34581895	4	19	theme	cellular	755:762	arg1	composition					764:774	the cellular composition	751:774	the cellular composition utilizing a novel approach for live-cell staining and direct imaging in the integrated microfluidic Stacks device	751:888	We then performed a comprehensive multi-parameter characterization of the cellular composition utilizing a novel approach for live-cell staining and direct imaging in the integrated microfluidic Stacks device.
34581895	6	20	theme	rare-event	1351:1360	arg1	protocol					1372:1379	a novel microfluidic rare-event screening protocol	1330:1379	a novel microfluidic rare-event screening protocol	1330:1379	Furthermore, to confirm the tumor-origin of the PDCOs we have analyzed the expression of PC-associated epigenetic biomarkers including promoter methylation of the GSTP1, RASSF1 and APC and RARb genes by employing a novel microfluidic rare-event screening protocol.
34581895	5	21	theme	elements	1107:1114	arg1	expression					1062:1071	expression	1062:1071	expression of androgen receptor (AR)-related elements	1062:1114	Using orthogonal flow cytometry analysis, we demonstrate that primary PDCOs maintain distinct subsets of epithelial cells throughout culture and that these cells conserve expression of androgen receptor (AR)-related elements.
34581895	6	22	theme	promoter	1252:1259	arg1	methylation					1261:1271	promoter methylation	1252:1271	promoter methylation of the GSTP1, RASSF1 and APC and RARb genes	1252:1315	Furthermore, to confirm the tumor-origin of the PDCOs we have analyzed the expression of PC-associated epigenetic biomarkers including promoter methylation of the GSTP1, RASSF1 and APC and RARb genes by employing a novel microfluidic rare-event screening protocol.
34581895	3	23	dep	ex	550:551	arg1	vivo					553:556	vivo	553:556	vivo	553:556	In current study, we established ex vivo primary patient-derived cancer organoid (PDCO) cultures from prostatectomy specimens of patients with locally advanced PC.
34581895	0	24	theme	organoids	56:64	arg1	analysis					20:27	Live cell molecular analysis	0:27	Live cell molecular analysis of primary prostate cancer organoids	0:64	Live cell molecular analysis of primary prostate cancer organoids identifies persistent androgen receptor signaling.
34581895	3	25	theme	prostatectomy	619:631	arg1	specimens					633:641	prostatectomy specimens	619:641	prostatectomy specimens of patients with locally advanced PC	619:678	In current study, we established ex vivo primary patient-derived cancer organoid (PDCO) cultures from prostatectomy specimens of patients with locally advanced PC.
34581895	2	26	theme	tumor	497:501	arg1	pathogenesis					503:514	tumor pathogenesis	497:514	tumor pathogenesis	497:514	Commonly available PC cell lines do not accurately reflect the complexity of this disease and there is critical need for development of new models to recapitulate the intricate hierarchy of tumor pathogenesis.
34581895	4	27	theme	multi-parameter	715:729	arg1	characterization					731:746	a comprehensive multi-parameter characterization	699:746	a comprehensive multi-parameter characterization of the cellular composition utilizing a novel approach for live-cell staining and direct imaging in the integrated microfluidic Stacks device	699:888	We then performed a comprehensive multi-parameter characterization of the cellular composition utilizing a novel approach for live-cell staining and direct imaging in the integrated microfluidic Stacks device.
34581895	0	28	theme	persistent	77:86	arg1	signaling					106:114	persistent androgen receptor signaling	77:114	persistent androgen receptor signaling	77:114	Live cell molecular analysis of primary prostate cancer organoids identifies persistent androgen receptor signaling.
34581895	5	29	theme	primary	953:959	arg1	PDCOs					961:965	primary PDCOs	953:965	primary PDCOs	953:965	Using orthogonal flow cytometry analysis, we demonstrate that primary PDCOs maintain distinct subsets of epithelial cells throughout culture and that these cells conserve expression of androgen receptor (AR)-related elements.
34581895	0	30	theme	receptor	97:104	arg1	signaling					106:114	persistent androgen receptor signaling	77:114	persistent androgen receptor signaling	77:114	Live cell molecular analysis of primary prostate cancer organoids identifies persistent androgen receptor signaling.
34581895	8	31	theme	microfluidic	1598:1609	arg1	device					1611:1616	the Stacks microfluidic device	1587:1616	the Stacks microfluidic device	1587:1616	Furthermore, we propose to leverage the Stacks microfluidic device as a high-throughput, translational platform to interrogate phenotypic and molecular endpoints with the capacity to incorporate a complex tumor microenvironment.
34581895	8	31	theme	microfluidic	1598:1609	arg1	platform					1654:1661	a high-throughput, translational platform	1621:1661	a high-throughput, translational platform to interrogate phenotypic and molecular endpoints with the capacity to incorporate a complex tumor microenvironment	1621:1777	Furthermore, we propose to leverage the Stacks microfluidic device as a high-throughput, translational platform to interrogate phenotypic and molecular endpoints with the capacity to incorporate a complex tumor microenvironment.
34581895	0	32	theme	Live	0:3	arg1	analysis					20:27	Live cell molecular analysis	0:27	Live cell molecular analysis of primary prostate cancer organoids	0:64	Live cell molecular analysis of primary prostate cancer organoids identifies persistent androgen receptor signaling.
34581895	1	33	theme	treatment	287:295	arg1	response					297:304	treatment response	287:304	treatment response	287:304	Prostate Cancer (PC) is a disease with remarkable tumor heterogeneity that often manifests in significant intra-patient variability with regards to clinical outcomes and treatment response.
34581895	5	34	theme	epithelial	996:1005	arg1	cells					1007:1011	epithelial cells	996:1011	epithelial cells	996:1011	Using orthogonal flow cytometry analysis, we demonstrate that primary PDCOs maintain distinct subsets of epithelial cells throughout culture and that these cells conserve expression of androgen receptor (AR)-related elements.
34581895	2	35	theme	new	443:445	arg1	models					447:452	new models	443:452	new models	443:452	Commonly available PC cell lines do not accurately reflect the complexity of this disease and there is critical need for development of new models to recapitulate the intricate hierarchy of tumor pathogenesis.
34581895	8	36	theme	high-throughput	1623:1637	arg1	device					1611:1616	the Stacks microfluidic device	1587:1616	the Stacks microfluidic device	1587:1616	Furthermore, we propose to leverage the Stacks microfluidic device as a high-throughput, translational platform to interrogate phenotypic and molecular endpoints with the capacity to incorporate a complex tumor microenvironment.
34581895	8	36	theme	high-throughput	1623:1637	arg1	platform					1654:1661	a high-throughput, translational platform	1621:1661	a high-throughput, translational platform to interrogate phenotypic and molecular endpoints with the capacity to incorporate a complex tumor microenvironment	1621:1777	Furthermore, we propose to leverage the Stacks microfluidic device as a high-throughput, translational platform to interrogate phenotypic and molecular endpoints with the capacity to incorporate a complex tumor microenvironment.
34581895	4	37	theme	novel	788:792	arg1	approach					794:801	a novel approach	786:801	a novel approach for live-cell staining and direct imaging in the integrated microfluidic Stacks device	786:888	We then performed a comprehensive multi-parameter characterization of the cellular composition utilizing a novel approach for live-cell staining and direct imaging in the integrated microfluidic Stacks device.
34581895	0	38	theme	molecular	10:18	arg1	analysis					20:27	Live cell molecular analysis	0:27	Live cell molecular analysis of primary prostate cancer organoids	0:64	Live cell molecular analysis of primary prostate cancer organoids identifies persistent androgen receptor signaling.
34581895	7	39	theme	microenvironment	1490:1505	arg1	complexity					1455:1464	the complexity	1451:1464	the complexity of the epithelial tumor microenvironment of multifocal PC using orthogonal analyses	1451:1548	These results demonstrate that this ex vivo PDCO model recapitulates the complexity of the epithelial tumor microenvironment of multifocal PC using orthogonal analyses.
34581895	1	40	theme	Prostate	117:124	arg1	PC					134:135	PC	134:135	PC	134:135	Prostate Cancer (PC) is a disease with remarkable tumor heterogeneity that often manifests in significant intra-patient variability with regards to clinical outcomes and treatment response.
34581895	1	40	theme	Prostate	117:124	arg1	Cancer					126:131	Prostate Cancer	117:131	Prostate Cancer (PC)	117:136	Prostate Cancer (PC) is a disease with remarkable tumor heterogeneity that often manifests in significant intra-patient variability with regards to clinical outcomes and treatment response.
34581895	1	40	theme	Prostate	117:124	arg1	disease					143:149	a disease	141:149	a disease with remarkable tumor heterogeneity that often manifests in significant intra-patient variability with regards to clinical outcomes and treatment response	141:304	Prostate Cancer (PC) is a disease with remarkable tumor heterogeneity that often manifests in significant intra-patient variability with regards to clinical outcomes and treatment response.
34581895	3	41	theme	advanced	668:675	arg1	PC					677:678	locally advanced PC	660:678	locally advanced PC	660:678	In current study, we established ex vivo primary patient-derived cancer organoid (PDCO) cultures from prostatectomy specimens of patients with locally advanced PC.
34581895	4	42	theme	integrated	852:861	arg1	device					883:888	the integrated microfluidic Stacks device	848:888	the integrated microfluidic Stacks device	848:888	We then performed a comprehensive multi-parameter characterization of the cellular composition utilizing a novel approach for live-cell staining and direct imaging in the integrated microfluidic Stacks device.
34581895	1	43	theme	remarkable	156:165	arg1	heterogeneity					173:185	remarkable tumor heterogeneity	156:185	remarkable tumor heterogeneity	156:185	Prostate Cancer (PC) is a disease with remarkable tumor heterogeneity that often manifests in significant intra-patient variability with regards to clinical outcomes and treatment response.
34581895	2	44	theme	pathogenesis	503:514	arg1	hierarchy					484:492	the intricate hierarchy	470:492	the intricate hierarchy of tumor pathogenesis	470:514	Commonly available PC cell lines do not accurately reflect the complexity of this disease and there is critical need for development of new models to recapitulate the intricate hierarchy of tumor pathogenesis.
34581895	4	45	theme	Stacks	876:881	arg1	device					883:888	the integrated microfluidic Stacks device	848:888	the integrated microfluidic Stacks device	848:888	We then performed a comprehensive multi-parameter characterization of the cellular composition utilizing a novel approach for live-cell staining and direct imaging in the integrated microfluidic Stacks device.
34581895	0	46	theme	prostate	40:47	arg1	organoids					56:64	primary prostate cancer organoids	32:64	primary prostate cancer organoids	32:64	Live cell molecular analysis of primary prostate cancer organoids identifies persistent androgen receptor signaling.
34581895	8	47	theme	phenotypic	1678:1687	arg1	endpoints					1703:1711	phenotypic and molecular endpoints	1678:1711	phenotypic and molecular endpoints	1678:1711	Furthermore, we propose to leverage the Stacks microfluidic device as a high-throughput, translational platform to interrogate phenotypic and molecular endpoints with the capacity to incorporate a complex tumor microenvironment.
34581895	4	48	theme	direct	830:835	arg1	imaging					837:843	direct imaging	830:843	direct imaging	830:843	We then performed a comprehensive multi-parameter characterization of the cellular composition utilizing a novel approach for live-cell staining and direct imaging in the integrated microfluidic Stacks device.
34581895	8	49	theme	complex	1748:1754	arg1	microenvironment					1762:1777	a complex tumor microenvironment	1746:1777	a complex tumor microenvironment	1746:1777	Furthermore, we propose to leverage the Stacks microfluidic device as a high-throughput, translational platform to interrogate phenotypic and molecular endpoints with the capacity to incorporate a complex tumor microenvironment.
34581895	1	50	theme	intra-patient	223:235	arg1	variability					237:247	significant intra-patient variability	211:247	significant intra-patient variability with regards to clinical outcomes and treatment response	211:304	Prostate Cancer (PC) is a disease with remarkable tumor heterogeneity that often manifests in significant intra-patient variability with regards to clinical outcomes and treatment response.
34581895	6	51	theme	PDCOs	1165:1169	arg1	tumor-origin					1145:1156	the tumor-origin	1141:1156	the tumor-origin of the PDCOs	1141:1169	Furthermore, to confirm the tumor-origin of the PDCOs we have analyzed the expression of PC-associated epigenetic biomarkers including promoter methylation of the GSTP1, RASSF1 and APC and RARb genes by employing a novel microfluidic rare-event screening protocol.
34581895	8	52	theme	molecular	1693:1701	arg1	endpoints					1703:1711	phenotypic and molecular endpoints	1678:1711	phenotypic and molecular endpoints	1678:1711	Furthermore, we propose to leverage the Stacks microfluidic device as a high-throughput, translational platform to interrogate phenotypic and molecular endpoints with the capacity to incorporate a complex tumor microenvironment.
34581895	6	53	dep	analyzed	1179:1186	arg1	confirm					1133:1139	confirm	1133:1139	to confirm the tumor-origin of the PDCOs	1130:1169	Furthermore, to confirm the tumor-origin of the PDCOs we have analyzed the expression of PC-associated epigenetic biomarkers including promoter methylation of the GSTP1, RASSF1 and APC and RARb genes by employing a novel microfluidic rare-event screening protocol.
34581895	5	54	theme	cytometry	913:921	arg1	analysis					923:930	orthogonal flow cytometry analysis	897:930	orthogonal flow cytometry analysis	897:930	Using orthogonal flow cytometry analysis, we demonstrate that primary PDCOs maintain distinct subsets of epithelial cells throughout culture and that these cells conserve expression of androgen receptor (AR)-related elements.
34581895	6	55	theme	microfluidic	1338:1349	arg1	protocol					1372:1379	a novel microfluidic rare-event screening protocol	1330:1379	a novel microfluidic rare-event screening protocol	1330:1379	Furthermore, to confirm the tumor-origin of the PDCOs we have analyzed the expression of PC-associated epigenetic biomarkers including promoter methylation of the GSTP1, RASSF1 and APC and RARb genes by employing a novel microfluidic rare-event screening protocol.
34581895	3	56	theme	ex	550:551	arg1	cultures					605:612	ex vivo primary patient-derived cancer organoid (PDCO) cultures	550:612	ex vivo primary patient-derived cancer organoid (PDCO) cultures from prostatectomy specimens of patients with locally advanced PC	550:678	In current study, we established ex vivo primary patient-derived cancer organoid (PDCO) cultures from prostatectomy specimens of patients with locally advanced PC.
34581895	7	57	theme	epithelial	1473:1482	arg1	microenvironment					1490:1505	the epithelial tumor microenvironment	1469:1505	the epithelial tumor microenvironment of multifocal PC using orthogonal analyses	1469:1548	These results demonstrate that this ex vivo PDCO model recapitulates the complexity of the epithelial tumor microenvironment of multifocal PC using orthogonal analyses.
34581895	7	58	theme	PC	1521:1522	arg1	microenvironment					1490:1505	the epithelial tumor microenvironment	1469:1505	the epithelial tumor microenvironment of multifocal PC using orthogonal analyses	1469:1548	These results demonstrate that this ex vivo PDCO model recapitulates the complexity of the epithelial tumor microenvironment of multifocal PC using orthogonal analyses.
34581895	5	59	theme	flow	908:911	arg1	analysis					923:930	orthogonal flow cytometry analysis	897:930	orthogonal flow cytometry analysis	897:930	Using orthogonal flow cytometry analysis, we demonstrate that primary PDCOs maintain distinct subsets of epithelial cells throughout culture and that these cells conserve expression of androgen receptor (AR)-related elements.
34581895	6	60	theme	epigenetic	1220:1229	arg1	biomarkers					1231:1240	PC-associated epigenetic biomarkers	1206:1240	PC-associated epigenetic biomarkers including promoter methylation of the GSTP1, RASSF1 and APC and RARb genes	1206:1315	Furthermore, to confirm the tumor-origin of the PDCOs we have analyzed the expression of PC-associated epigenetic biomarkers including promoter methylation of the GSTP1, RASSF1 and APC and RARb genes by employing a novel microfluidic rare-event screening protocol.
34581895	6	60	theme	epigenetic	1220:1229	arg1	methylation					1261:1271	promoter methylation	1252:1271	promoter methylation of the GSTP1, RASSF1 and APC and RARb genes	1252:1315	Furthermore, to confirm the tumor-origin of the PDCOs we have analyzed the expression of PC-associated epigenetic biomarkers including promoter methylation of the GSTP1, RASSF1 and APC and RARb genes by employing a novel microfluidic rare-event screening protocol.
34581895	3	61	theme	cancer	582:587	arg1	cultures					605:612	ex vivo primary patient-derived cancer organoid (PDCO) cultures	550:612	ex vivo primary patient-derived cancer organoid (PDCO) cultures from prostatectomy specimens of patients with locally advanced PC	550:678	In current study, we established ex vivo primary patient-derived cancer organoid (PDCO) cultures from prostatectomy specimens of patients with locally advanced PC.
34581895	7	62	theme	tumor	1484:1488	arg1	microenvironment					1490:1505	the epithelial tumor microenvironment	1469:1505	the epithelial tumor microenvironment of multifocal PC using orthogonal analyses	1469:1548	These results demonstrate that this ex vivo PDCO model recapitulates the complexity of the epithelial tumor microenvironment of multifocal PC using orthogonal analyses.
34581895	2	63	theme	PC	326:327	arg1	lines					334:338	Commonly available PC cell lines	307:338	Commonly available PC cell lines	307:338	Commonly available PC cell lines do not accurately reflect the complexity of this disease and there is critical need for development of new models to recapitulate the intricate hierarchy of tumor pathogenesis.
34581895	3	64	theme	organoid	589:596	arg1	cultures					605:612	ex vivo primary patient-derived cancer organoid (PDCO) cultures	550:612	ex vivo primary patient-derived cancer organoid (PDCO) cultures from prostatectomy specimens of patients with locally advanced PC	550:678	In current study, we established ex vivo primary patient-derived cancer organoid (PDCO) cultures from prostatectomy specimens of patients with locally advanced PC.
34581895	6	65	theme	screening	1362:1370	arg1	protocol					1372:1379	a novel microfluidic rare-event screening protocol	1330:1379	a novel microfluidic rare-event screening protocol	1330:1379	Furthermore, to confirm the tumor-origin of the PDCOs we have analyzed the expression of PC-associated epigenetic biomarkers including promoter methylation of the GSTP1, RASSF1 and APC and RARb genes by employing a novel microfluidic rare-event screening protocol.
34581895	5	66	theme	-related	1098:1105	arg1	elements					1107:1114	androgen receptor (AR)-related elements	1076:1114	androgen receptor (AR)-related elements	1076:1114	Using orthogonal flow cytometry analysis, we demonstrate that primary PDCOs maintain distinct subsets of epithelial cells throughout culture and that these cells conserve expression of androgen receptor (AR)-related elements.
34581895	5	67	theme	distinct	976:983	arg1	subsets					985:991	distinct subsets	976:991	distinct subsets of epithelial cells	976:1011	Using orthogonal flow cytometry analysis, we demonstrate that primary PDCOs maintain distinct subsets of epithelial cells throughout culture and that these cells conserve expression of androgen receptor (AR)-related elements.
34581895	3	68	theme	primary	558:564	arg1	cultures					605:612	ex vivo primary patient-derived cancer organoid (PDCO) cultures	550:612	ex vivo primary patient-derived cancer organoid (PDCO) cultures from prostatectomy specimens of patients with locally advanced PC	550:678	In current study, we established ex vivo primary patient-derived cancer organoid (PDCO) cultures from prostatectomy specimens of patients with locally advanced PC.
34581895	7	69	theme	PDCO	1426:1429	arg1	model					1431:1435	this ex vivo PDCO model	1413:1435	this ex vivo PDCO model	1413:1435	These results demonstrate that this ex vivo PDCO model recapitulates the complexity of the epithelial tumor microenvironment of multifocal PC using orthogonal analyses.
34581895	8	70	dep	high-throughput	1623:1637	arg1	translational					1640:1652	translational	1640:1652	translational	1640:1652	Furthermore, we propose to leverage the Stacks microfluidic device as a high-throughput, translational platform to interrogate phenotypic and molecular endpoints with the capacity to incorporate a complex tumor microenvironment.
34581895	3	71	link	patient-derived	566:580	arg1	cultures					605:612	ex vivo primary patient-derived cancer organoid (PDCO) cultures	550:612	ex vivo primary patient-derived cancer organoid (PDCO) cultures from prostatectomy specimens of patients with locally advanced PC	550:678	In current study, we established ex vivo primary patient-derived cancer organoid (PDCO) cultures from prostatectomy specimens of patients with locally advanced PC.
34581895	7	72	theme	ex	1418:1419	arg1	model					1431:1435	this ex vivo PDCO model	1413:1435	this ex vivo PDCO model	1413:1435	These results demonstrate that this ex vivo PDCO model recapitulates the complexity of the epithelial tumor microenvironment of multifocal PC using orthogonal analyses.
34581895	0	73	theme	androgen	88:95	arg1	signaling					106:114	persistent androgen receptor signaling	77:114	persistent androgen receptor signaling	77:114	Live cell molecular analysis of primary prostate cancer organoids identifies persistent androgen receptor signaling.
34581895	1	74	with	disease	143:149	arg1	heterogeneity					173:185	remarkable tumor heterogeneity	156:185	remarkable tumor heterogeneity	156:185	Prostate Cancer (PC) is a disease with remarkable tumor heterogeneity that often manifests in significant intra-patient variability with regards to clinical outcomes and treatment response.
34581895	8	75	theme	Stacks	1591:1596	arg1	device					1611:1616	the Stacks microfluidic device	1587:1616	the Stacks microfluidic device	1587:1616	Furthermore, we propose to leverage the Stacks microfluidic device as a high-throughput, translational platform to interrogate phenotypic and molecular endpoints with the capacity to incorporate a complex tumor microenvironment.
34581895	8	75	theme	Stacks	1591:1596	arg1	platform					1654:1661	a high-throughput, translational platform	1621:1661	a high-throughput, translational platform to interrogate phenotypic and molecular endpoints with the capacity to incorporate a complex tumor microenvironment	1621:1777	Furthermore, we propose to leverage the Stacks microfluidic device as a high-throughput, translational platform to interrogate phenotypic and molecular endpoints with the capacity to incorporate a complex tumor microenvironment.
34581895	4	76	theme	comprehensive	701:713	arg1	characterization					731:746	a comprehensive multi-parameter characterization	699:746	a comprehensive multi-parameter characterization of the cellular composition utilizing a novel approach for live-cell staining and direct imaging in the integrated microfluidic Stacks device	699:888	We then performed a comprehensive multi-parameter characterization of the cellular composition utilizing a novel approach for live-cell staining and direct imaging in the integrated microfluidic Stacks device.
34581895	3	77	theme	patient-derived	566:580	arg1	cultures					605:612	ex vivo primary patient-derived cancer organoid (PDCO) cultures	550:612	ex vivo primary patient-derived cancer organoid (PDCO) cultures from prostatectomy specimens of patients with locally advanced PC	550:678	In current study, we established ex vivo primary patient-derived cancer organoid (PDCO) cultures from prostatectomy specimens of patients with locally advanced PC.
34581895	4	78	from	approach	794:801	arg1	device					883:888	the integrated microfluidic Stacks device	848:888	the integrated microfluidic Stacks device	848:888	We then performed a comprehensive multi-parameter characterization of the cellular composition utilizing a novel approach for live-cell staining and direct imaging in the integrated microfluidic Stacks device.
34581895	4	79	theme	live-cell	807:815	arg1	staining					817:824	live-cell staining	807:824	live-cell staining	807:824	We then performed a comprehensive multi-parameter characterization of the cellular composition utilizing a novel approach for live-cell staining and direct imaging in the integrated microfluidic Stacks device.
34581895	3	80	from	specimens	633:641	arg1	cultures					605:612	ex vivo primary patient-derived cancer organoid (PDCO) cultures	550:612	ex vivo primary patient-derived cancer organoid (PDCO) cultures from prostatectomy specimens of patients with locally advanced PC	550:678	In current study, we established ex vivo primary patient-derived cancer organoid (PDCO) cultures from prostatectomy specimens of patients with locally advanced PC.
34581895	0	81	theme	cell	5:8	arg1	analysis					20:27	Live cell molecular analysis	0:27	Live cell molecular analysis of primary prostate cancer organoids	0:64	Live cell molecular analysis of primary prostate cancer organoids identifies persistent androgen receptor signaling.
34581895	2	82	theme	models	447:452	arg1	development					428:438	development	428:438	development of new models	428:452	Commonly available PC cell lines do not accurately reflect the complexity of this disease and there is critical need for development of new models to recapitulate the intricate hierarchy of tumor pathogenesis.
34581895	4	83	theme	composition	764:774	arg1	characterization					731:746	a comprehensive multi-parameter characterization	699:746	a comprehensive multi-parameter characterization of the cellular composition utilizing a novel approach for live-cell staining and direct imaging in the integrated microfluidic Stacks device	699:888	We then performed a comprehensive multi-parameter characterization of the cellular composition utilizing a novel approach for live-cell staining and direct imaging in the integrated microfluidic Stacks device.
34581895	2	84	theme	disease	389:395	arg1	complexity					370:379	the complexity	366:379	the complexity of this disease	366:395	Commonly available PC cell lines do not accurately reflect the complexity of this disease and there is critical need for development of new models to recapitulate the intricate hierarchy of tumor pathogenesis.
34581895	5	85	theme	cells	1007:1011	arg1	subsets					985:991	distinct subsets	976:991	distinct subsets of epithelial cells	976:1011	Using orthogonal flow cytometry analysis, we demonstrate that primary PDCOs maintain distinct subsets of epithelial cells throughout culture and that these cells conserve expression of androgen receptor (AR)-related elements.
34581895	0	86	theme	primary	32:38	arg1	organoids					56:64	primary prostate cancer organoids	32:64	primary prostate cancer organoids	32:64	Live cell molecular analysis of primary prostate cancer organoids identifies persistent androgen receptor signaling.
34581895	5	87	theme	androgen	1076:1083	arg1	receptor					1085:1092	androgen receptor	1076:1092	androgen receptor (AR)	1076:1097	Using orthogonal flow cytometry analysis, we demonstrate that primary PDCOs maintain distinct subsets of epithelial cells throughout culture and that these cells conserve expression of androgen receptor (AR)-related elements.
34581895	5	87	theme	androgen	1076:1083	arg1	AR					1095:1096	AR	1095:1096	AR	1095:1096	Using orthogonal flow cytometry analysis, we demonstrate that primary PDCOs maintain distinct subsets of epithelial cells throughout culture and that these cells conserve expression of androgen receptor (AR)-related elements.
34581895	2	88	theme	intricate	474:482	arg1	hierarchy					484:492	the intricate hierarchy	470:492	the intricate hierarchy of tumor pathogenesis	470:514	Commonly available PC cell lines do not accurately reflect the complexity of this disease and there is critical need for development of new models to recapitulate the intricate hierarchy of tumor pathogenesis.
34581895	3	89	theme	patients	646:653	arg1	specimens					633:641	prostatectomy specimens	619:641	prostatectomy specimens of patients with locally advanced PC	619:678	In current study, we established ex vivo primary patient-derived cancer organoid (PDCO) cultures from prostatectomy specimens of patients with locally advanced PC.
34581895	2	90	theme	critical	410:417	arg1	need					419:422	critical need	410:422	critical need for development of new models to recapitulate the intricate hierarchy of tumor pathogenesis	410:514	Commonly available PC cell lines do not accurately reflect the complexity of this disease and there is critical need for development of new models to recapitulate the intricate hierarchy of tumor pathogenesis.
34581895	0	91	theme	cancer	49:54	arg1	organoids					56:64	primary prostate cancer organoids	32:64	primary prostate cancer organoids	32:64	Live cell molecular analysis of primary prostate cancer organoids identifies persistent androgen receptor signaling.
34581895	4	92	theme	microfluidic	863:874	arg1	device					883:888	the integrated microfluidic Stacks device	848:888	the integrated microfluidic Stacks device	848:888	We then performed a comprehensive multi-parameter characterization of the cellular composition utilizing a novel approach for live-cell staining and direct imaging in the integrated microfluidic Stacks device.
34581895	8	93	theme	tumor	1756:1760	arg1	microenvironment					1762:1777	a complex tumor microenvironment	1746:1777	a complex tumor microenvironment	1746:1777	Furthermore, we propose to leverage the Stacks microfluidic device as a high-throughput, translational platform to interrogate phenotypic and molecular endpoints with the capacity to incorporate a complex tumor microenvironment.
34581895	1	94	theme	significant	211:221	arg1	variability					237:247	significant intra-patient variability	211:247	significant intra-patient variability with regards to clinical outcomes and treatment response	211:304	Prostate Cancer (PC) is a disease with remarkable tumor heterogeneity that often manifests in significant intra-patient variability with regards to clinical outcomes and treatment response.
32798183	3	0	theme	respiratory	639:649	arg1	droplets					651:658	respiratory droplets	639:658	respiratory droplets	639:658	The primary route of SARS-CoV-2 transmission from human to human is through inhalation of respiratory droplets.
32798183	4	1	theme	vital	781:785	arg1	requirement					787:797	a vital requirement	779:797	a vital requirement to curb the ongoing outbreak	779:826	Devising protective technologies for stopping the spread of the droplets of aerosol containing the viral particles is a vital requirement to curb the ongoing outbreak.
32798183	8	2	theme	nanofibrous	1403:1413	arg1	matrix					1415:1420	a nanofibrous matrix	1401:1420	a nanofibrous matrix of polylactic acid and cellulose acetate containing copper oxide nanoparticles and graphene oxide nanosheets and produced using the electrospinning technique	1401:1578	The novel design is based on the filtration system composed of a nanofibrous matrix of polylactic acid and cellulose acetate containing copper oxide nanoparticles and graphene oxide nanosheets and produced using the electrospinning technique.
32798183	1	3	theme	coronavirus	136:146	arg1	COVID-19					162:169	COVID-19	162:169	COVID-19	162:169	The outbreak of the coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is thought to have occurred first in Wuhan, China in December 2019, before spreading to over 120 countries in the months that followed.
32798183	1	3	theme	coronavirus	136:146	arg1	disease					148:154	the coronavirus disease 2019	132:159	the coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	132:241	The outbreak of the coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is thought to have occurred first in Wuhan, China in December 2019, before spreading to over 120 countries in the months that followed.
32798183	5	4	from	use	896:898	arg1	generations					850:860	the current generations	838:860	the current generations of protective respirator masks in use	838:898	However, the current generations of protective respirator masks in use are noted for their imperfect design and there is a need to develop their more advanced analogues, with higher blockage efficiency and the ability to deactivate the trapped bacteria and viruses.
32798183	8	5	theme	cellulose	1445:1453	arg1	acetate					1455:1461	cellulose acetate	1445:1461	cellulose acetate containing copper oxide nanoparticles and graphene oxide nanosheets and produced using the electrospinning technique	1445:1578	The novel design is based on the filtration system composed of a nanofibrous matrix of polylactic acid and cellulose acetate containing copper oxide nanoparticles and graphene oxide nanosheets and produced using the electrospinning technique.
32798183	1	6	theme	disease	148:154	arg1	outbreak					120:127	The outbreak	116:127	The outbreak of the coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	116:241	The outbreak of the coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is thought to have occurred first in Wuhan, China in December 2019, before spreading to over 120 countries in the months that followed.
32798183	5	7	from	respirator	876:885	arg1	use					896:898	use	896:898	use	896:898	However, the current generations of protective respirator masks in use are noted for their imperfect design and there is a need to develop their more advanced analogues, with higher blockage efficiency and the ability to deactivate the trapped bacteria and viruses.
32798183	4	8	contain	containing	745:754	arg1	aerosol					737:743	aerosol	737:743	aerosol containing the viral particles	737:774	Devising protective technologies for stopping the spread of the droplets of aerosol containing the viral particles is a vital requirement to curb the ongoing outbreak.
32798183	4	8	contain	containing	745:754	arg2	particles					766:774	the viral particles	756:774	the viral particles	756:774	Devising protective technologies for stopping the spread of the droplets of aerosol containing the viral particles is a vital requirement to curb the ongoing outbreak.
32798183	0	9	theme	new	93:95	arg1	device					97:102	a new device	91:102	a new device from Egypt	91:113	Protecting healthcare workers during COVID-19 pandemic with nanotechnology: A protocol for a new device from Egypt.
32798183	8	10	theme	polylactic	1425:1434	arg1	acid					1436:1439	polylactic acid	1425:1439	polylactic acid	1425:1439	The novel design is based on the filtration system composed of a nanofibrous matrix of polylactic acid and cellulose acetate containing copper oxide nanoparticles and graphene oxide nanosheets and produced using the electrospinning technique.
32798183	5	11	theme	respirator	876:885	arg1	generations					850:860	the current generations	838:860	the current generations of protective respirator masks in use	838:898	However, the current generations of protective respirator masks in use are noted for their imperfect design and there is a need to develop their more advanced analogues, with higher blockage efficiency and the ability to deactivate the trapped bacteria and viruses.
32798183	7	12	theme	antiviral	1275:1283	arg1	mask					1303:1306	a reusable, recyclable, customizable, antimicrobial and antiviral respirator facial mask	1219:1306	a reusable, recyclable, customizable, antimicrobial and antiviral respirator facial mask feasible for mass production	1219:1335	Here we describe a new design from Egypt, utilizing a reusable, recyclable, customizable, antimicrobial and antiviral respirator facial mask feasible for mass production.
32798183	9	13	used	used	1668:1671	arg2	pattern					1606:1612	the flat pattern	1597:1612	the flat pattern fabricated from a thermoplastic composite material	1597:1663	Simultaneously, the flat pattern fabricated from a thermoplastic composite material is used to provide a solid fit with the facial anatomy.
32798183	7	14	theme	respirator	1285:1294	arg1	mask					1303:1306	a reusable, recyclable, customizable, antimicrobial and antiviral respirator facial mask	1219:1306	a reusable, recyclable, customizable, antimicrobial and antiviral respirator facial mask feasible for mass production	1219:1335	Here we describe a new design from Egypt, utilizing a reusable, recyclable, customizable, antimicrobial and antiviral respirator facial mask feasible for mass production.
32798183	7	15	theme	mass	1321:1324	arg1	production					1326:1335	mass production	1321:1335	mass production	1321:1335	Here we describe a new design from Egypt, utilizing a reusable, recyclable, customizable, antimicrobial and antiviral respirator facial mask feasible for mass production.
32798183	10	16	theme	global	1869:1874	arg1	significance					1876:1887	potentially global significance	1857:1887	potentially global significance	1857:1887	This design illustrates an effort made in a developing setting to provide innovative solutions for combating the SARS-CoV-2 pandemic of potentially global significance.
32798183	4	17	theme	protective	670:679	arg1	technologies					681:692	protective technologies	670:692	protective technologies	670:692	Devising protective technologies for stopping the spread of the droplets of aerosol containing the viral particles is a vital requirement to curb the ongoing outbreak.
32798183	8	18	theme	acid	1436:1439	arg1	matrix					1415:1420	a nanofibrous matrix	1401:1420	a nanofibrous matrix of polylactic acid and cellulose acetate containing copper oxide nanoparticles and graphene oxide nanosheets and produced using the electrospinning technique	1401:1578	The novel design is based on the filtration system composed of a nanofibrous matrix of polylactic acid and cellulose acetate containing copper oxide nanoparticles and graphene oxide nanosheets and produced using the electrospinning technique.
32798183	4	19	theme	viral	760:764	arg1	particles					766:774	the viral particles	756:774	the viral particles	756:774	Devising protective technologies for stopping the spread of the droplets of aerosol containing the viral particles is a vital requirement to curb the ongoing outbreak.
32798183	10	20	theme	SARS-CoV-2	1834:1843	arg1	pandemic					1845:1852	the SARS-CoV-2 pandemic	1830:1852	the SARS-CoV-2 pandemic of potentially global significance	1830:1887	This design illustrates an effort made in a developing setting to provide innovative solutions for combating the SARS-CoV-2 pandemic of potentially global significance.
32798183	7	21	theme	feasible	1308:1315	arg1	mask					1303:1306	a reusable, recyclable, customizable, antimicrobial and antiviral respirator facial mask	1219:1306	a reusable, recyclable, customizable, antimicrobial and antiviral respirator facial mask feasible for mass production	1219:1335	Here we describe a new design from Egypt, utilizing a reusable, recyclable, customizable, antimicrobial and antiviral respirator facial mask feasible for mass production.
32798183	0	22	theme	healthcare	11:20	arg1	workers					22:28	healthcare workers	11:28	healthcare workers	11:28	Protecting healthcare workers during COVID-19 pandemic with nanotechnology: A protocol for a new device from Egypt.
32798183	5	23	theme	higher	1004:1009	arg1	efficiency					1020:1029	higher blockage efficiency	1004:1029	higher blockage efficiency	1004:1029	However, the current generations of protective respirator masks in use are noted for their imperfect design and there is a need to develop their more advanced analogues, with higher blockage efficiency and the ability to deactivate the trapped bacteria and viruses.
32798183	3	24	from	route	561:565	arg1	human					599:603	human	599:603	human	599:603	The primary route of SARS-CoV-2 transmission from human to human is through inhalation of respiratory droplets.
32798183	5	25	theme	current	842:848	arg1	generations					850:860	the current generations	838:860	the current generations of protective respirator masks in use	838:898	However, the current generations of protective respirator masks in use are noted for their imperfect design and there is a need to develop their more advanced analogues, with higher blockage efficiency and the ability to deactivate the trapped bacteria and viruses.
32798183	9	26	theme	thermoplastic	1632:1644	arg1	material					1656:1663	a thermoplastic composite material	1630:1663	a thermoplastic composite material	1630:1663	Simultaneously, the flat pattern fabricated from a thermoplastic composite material is used to provide a solid fit with the facial anatomy.
32798183	9	27	theme	facial	1705:1710	arg1	anatomy					1712:1718	the facial anatomy	1701:1718	the facial anatomy	1701:1718	Simultaneously, the flat pattern fabricated from a thermoplastic composite material is used to provide a solid fit with the facial anatomy.
32798183	5	28	theme	trapped	1065:1071	arg1	bacteria					1073:1080	bacteria	1073:1080	bacteria	1073:1080	However, the current generations of protective respirator masks in use are noted for their imperfect design and there is a need to develop their more advanced analogues, with higher blockage efficiency and the ability to deactivate the trapped bacteria and viruses.
32798183	7	29	theme	facial	1296:1301	arg1	mask					1303:1306	a reusable, recyclable, customizable, antimicrobial and antiviral respirator facial mask	1219:1306	a reusable, recyclable, customizable, antimicrobial and antiviral respirator facial mask feasible for mass production	1219:1335	Here we describe a new design from Egypt, utilizing a reusable, recyclable, customizable, antimicrobial and antiviral respirator facial mask feasible for mass production.
32798183	9	30	theme	solid	1686:1690	arg1	fit					1692:1694	a solid fit	1684:1694	a solid fit with the facial anatomy	1684:1718	Simultaneously, the flat pattern fabricated from a thermoplastic composite material is used to provide a solid fit with the facial anatomy.
32798183	10	31	theme	significance	1876:1887	arg1	pandemic					1845:1852	the SARS-CoV-2 pandemic	1830:1852	the SARS-CoV-2 pandemic of potentially global significance	1830:1887	This design illustrates an effort made in a developing setting to provide innovative solutions for combating the SARS-CoV-2 pandemic of potentially global significance.
32798183	9	32	theme	flat	1601:1604	arg1	pattern					1606:1612	the flat pattern	1597:1612	the flat pattern fabricated from a thermoplastic composite material	1597:1663	Simultaneously, the flat pattern fabricated from a thermoplastic composite material is used to provide a solid fit with the facial anatomy.
32798183	7	33	theme	customizable	1243:1254	arg1	mask					1303:1306	a reusable, recyclable, customizable, antimicrobial and antiviral respirator facial mask	1219:1306	a reusable, recyclable, customizable, antimicrobial and antiviral respirator facial mask feasible for mass production	1219:1335	Here we describe a new design from Egypt, utilizing a reusable, recyclable, customizable, antimicrobial and antiviral respirator facial mask feasible for mass production.
32798183	9	34	with	fit	1692:1694	arg1	anatomy					1712:1718	the facial anatomy	1701:1718	the facial anatomy	1701:1718	Simultaneously, the flat pattern fabricated from a thermoplastic composite material is used to provide a solid fit with the facial anatomy.
32798183	2	35	theme	World	455:459	arg1	Organization					468:479	the World Health Organization	451:479	the World Health Organization	451:479	It was declared a "public health emergency of international concern" by the World Health Organization on January 31, 2020 and recognized as a pandemic on March 11, 2020.
32798183	2	36	from	pandemic	521:528	arg1	March					533:537	March	533:537	March	533:537	It was declared a "public health emergency of international concern" by the World Health Organization on January 31, 2020 and recognized as a pandemic on March 11, 2020.
32798183	5	37	theme	protective	865:874	arg1	respirator					876:885	protective respirator masks	865:891	protective respirator masks in use	865:898	However, the current generations of protective respirator masks in use are noted for their imperfect design and there is a need to develop their more advanced analogues, with higher blockage efficiency and the ability to deactivate the trapped bacteria and viruses.
32798183	2	38	theme	health	405:410	arg1	emergency					412:420	a "public health emergency	395:420	a "public health emergency of international concern	395:445	It was declared a "public health emergency of international concern" by the World Health Organization on January 31, 2020 and recognized as a pandemic on March 11, 2020.
32798183	3	39	theme	droplets	651:658	arg1	inhalation					625:634	inhalation	625:634	inhalation of respiratory droplets	625:658	The primary route of SARS-CoV-2 transmission from human to human is through inhalation of respiratory droplets.
32798183	3	40	theme	primary	553:559	arg1	route					561:565	The primary route	549:565	The primary route of SARS-CoV-2 transmission from human to human	549:612	The primary route of SARS-CoV-2 transmission from human to human is through inhalation of respiratory droplets.
32798183	5	41	theme	imperfect	920:928	arg1	design					930:935	their imperfect design	914:935	their imperfect design	914:935	However, the current generations of protective respirator masks in use are noted for their imperfect design and there is a need to develop their more advanced analogues, with higher blockage efficiency and the ability to deactivate the trapped bacteria and viruses.
32798183	8	42	theme	electrospinning	1554:1568	arg1	technique					1570:1578	the electrospinning technique	1550:1578	the electrospinning technique	1550:1578	The novel design is based on the filtration system composed of a nanofibrous matrix of polylactic acid and cellulose acetate containing copper oxide nanoparticles and graphene oxide nanosheets and produced using the electrospinning technique.
32798183	1	43	theme	severe	182:187	arg1	coronavirus					216:226	severe acute respiratory syndrome coronavirus 2	182:228	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	182:241	The outbreak of the coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is thought to have occurred first in Wuhan, China in December 2019, before spreading to over 120 countries in the months that followed.
32798183	1	43	theme	severe	182:187	arg1	SARS-CoV-2					231:240	SARS-CoV-2	231:240	SARS-CoV-2	231:240	The outbreak of the coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is thought to have occurred first in Wuhan, China in December 2019, before spreading to over 120 countries in the months that followed.
32798183	2	44	theme	public	398:403	arg1	emergency					412:420	a "public health emergency	395:420	a "public health emergency of international concern	395:445	It was declared a "public health emergency of international concern" by the World Health Organization on January 31, 2020 and recognized as a pandemic on March 11, 2020.
32798183	3	45	from	human	599:603	arg1	route					561:565	The primary route	549:565	The primary route of SARS-CoV-2 transmission from human to human	549:612	The primary route of SARS-CoV-2 transmission from human to human is through inhalation of respiratory droplets.
32798183	3	45	from	human	599:603	arg1	transmission					581:592	SARS-CoV-2 transmission	570:592	SARS-CoV-2 transmission from human to human	570:612	The primary route of SARS-CoV-2 transmission from human to human is through inhalation of respiratory droplets.
32798183	0	46	theme	COVID-19	37:44	arg1	pandemic					46:53	COVID-19 pandemic	37:53	COVID-19 pandemic with nanotechnology	37:73	Protecting healthcare workers during COVID-19 pandemic with nanotechnology: A protocol for a new device from Egypt.
32798183	8	47	theme	novel	1342:1346	arg1	design					1348:1353	The novel design	1338:1353	The novel design	1338:1353	The novel design is based on the filtration system composed of a nanofibrous matrix of polylactic acid and cellulose acetate containing copper oxide nanoparticles and graphene oxide nanosheets and produced using the electrospinning technique.
32798183	4	48	theme	droplets	725:732	arg1	spread					711:716	the spread	707:716	the spread of the droplets of aerosol containing the viral particles	707:774	Devising protective technologies for stopping the spread of the droplets of aerosol containing the viral particles is a vital requirement to curb the ongoing outbreak.
32798183	1	49	theme	acute	189:193	arg1	coronavirus					216:226	severe acute respiratory syndrome coronavirus 2	182:228	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	182:241	The outbreak of the coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is thought to have occurred first in Wuhan, China in December 2019, before spreading to over 120 countries in the months that followed.
32798183	1	49	theme	acute	189:193	arg1	SARS-CoV-2					231:240	SARS-CoV-2	231:240	SARS-CoV-2	231:240	The outbreak of the coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is thought to have occurred first in Wuhan, China in December 2019, before spreading to over 120 countries in the months that followed.
32798183	1	50	from	countries	340:348	arg1	months					357:362	the months	353:362	the months that followed	353:376	The outbreak of the coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is thought to have occurred first in Wuhan, China in December 2019, before spreading to over 120 countries in the months that followed.
32798183	5	51	theme	advanced	979:986	arg1	analogues					988:996	their more advanced analogues	968:996	their more advanced analogues	968:996	However, the current generations of protective respirator masks in use are noted for their imperfect design and there is a need to develop their more advanced analogues, with higher blockage efficiency and the ability to deactivate the trapped bacteria and viruses.
32798183	9	52	theme	composite	1646:1654	arg1	material					1656:1663	a thermoplastic composite material	1630:1663	a thermoplastic composite material	1630:1663	Simultaneously, the flat pattern fabricated from a thermoplastic composite material is used to provide a solid fit with the facial anatomy.
32798183	8	53	theme	oxide	1481:1485	arg1	nanoparticles					1487:1499	copper oxide nanoparticles	1474:1499	copper oxide nanoparticles	1474:1499	The novel design is based on the filtration system composed of a nanofibrous matrix of polylactic acid and cellulose acetate containing copper oxide nanoparticles and graphene oxide nanosheets and produced using the electrospinning technique.
32798183	2	54	theme	Health	461:466	arg1	Organization					468:479	the World Health Organization	451:479	the World Health Organization	451:479	It was declared a "public health emergency of international concern" by the World Health Organization on January 31, 2020 and recognized as a pandemic on March 11, 2020.
32798183	8	55	theme	copper	1474:1479	arg1	nanoparticles					1487:1499	copper oxide nanoparticles	1474:1499	copper oxide nanoparticles	1474:1499	The novel design is based on the filtration system composed of a nanofibrous matrix of polylactic acid and cellulose acetate containing copper oxide nanoparticles and graphene oxide nanosheets and produced using the electrospinning technique.
32798183	8	56	theme	filtration	1371:1380	arg1	system					1382:1387	the filtration system	1367:1387	the filtration system composed of a nanofibrous matrix of polylactic acid and cellulose acetate containing copper oxide nanoparticles and graphene oxide nanosheets and produced using the electrospinning technique	1367:1578	The novel design is based on the filtration system composed of a nanofibrous matrix of polylactic acid and cellulose acetate containing copper oxide nanoparticles and graphene oxide nanosheets and produced using the electrospinning technique.
32798183	3	57	theme	SARS-CoV-2	570:579	arg1	transmission					581:592	SARS-CoV-2 transmission	570:592	SARS-CoV-2 transmission from human to human	570:612	The primary route of SARS-CoV-2 transmission from human to human is through inhalation of respiratory droplets.
32798183	4	58	theme	aerosol	737:743	arg1	droplets					725:732	the droplets	721:732	the droplets of aerosol containing the viral particles	721:774	Devising protective technologies for stopping the spread of the droplets of aerosol containing the viral particles is a vital requirement to curb the ongoing outbreak.
32798183	10	59	theme	innovative	1795:1804	arg1	solutions					1806:1814	innovative solutions	1795:1814	innovative solutions	1795:1814	This design illustrates an effort made in a developing setting to provide innovative solutions for combating the SARS-CoV-2 pandemic of potentially global significance.
32798183	8	60	dep	acetate	1455:1461	arg1	containing					1463:1472	containing	1463:1472	containing copper oxide nanoparticles and graphene oxide nanosheets	1463:1529	The novel design is based on the filtration system composed of a nanofibrous matrix of polylactic acid and cellulose acetate containing copper oxide nanoparticles and graphene oxide nanosheets and produced using the electrospinning technique.
32798183	8	60	dep	acetate	1455:1461	arg1	produced					1535:1542	produced	1535:1542	produced using the electrospinning technique	1535:1578	The novel design is based on the filtration system composed of a nanofibrous matrix of polylactic acid and cellulose acetate containing copper oxide nanoparticles and graphene oxide nanosheets and produced using the electrospinning technique.
32798183	7	61	theme	reusable	1221:1228	arg1	mask					1303:1306	a reusable, recyclable, customizable, antimicrobial and antiviral respirator facial mask	1219:1306	a reusable, recyclable, customizable, antimicrobial and antiviral respirator facial mask feasible for mass production	1219:1335	Here we describe a new design from Egypt, utilizing a reusable, recyclable, customizable, antimicrobial and antiviral respirator facial mask feasible for mass production.
32798183	8	62	theme	oxide	1514:1518	arg1	nanosheets					1520:1529	graphene oxide nanosheets	1505:1529	graphene oxide nanosheets	1505:1529	The novel design is based on the filtration system composed of a nanofibrous matrix of polylactic acid and cellulose acetate containing copper oxide nanoparticles and graphene oxide nanosheets and produced using the electrospinning technique.
32798183	3	63	theme	transmission	581:592	arg1	route					561:565	The primary route	549:565	The primary route of SARS-CoV-2 transmission from human to human	549:612	The primary route of SARS-CoV-2 transmission from human to human is through inhalation of respiratory droplets.
32798183	6	64	theme	such	1117:1120	arg1	design					1122:1127	one such design	1113:1127	one such design	1113:1127	It is likely that one such design will be inspired by nanotechnologies.
32798183	5	65	dep	respirator	876:885	arg1	masks					887:891	masks	887:891	masks	887:891	However, the current generations of protective respirator masks in use are noted for their imperfect design and there is a need to develop their more advanced analogues, with higher blockage efficiency and the ability to deactivate the trapped bacteria and viruses.
32798183	1	66	theme	respiratory	195:205	arg1	coronavirus					216:226	severe acute respiratory syndrome coronavirus 2	182:228	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	182:241	The outbreak of the coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is thought to have occurred first in Wuhan, China in December 2019, before spreading to over 120 countries in the months that followed.
32798183	1	66	theme	respiratory	195:205	arg1	SARS-CoV-2					231:240	SARS-CoV-2	231:240	SARS-CoV-2	231:240	The outbreak of the coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is thought to have occurred first in Wuhan, China in December 2019, before spreading to over 120 countries in the months that followed.
32798183	4	67	theme	ongoing	811:817	arg1	outbreak					819:826	the ongoing outbreak	807:826	the ongoing outbreak	807:826	Devising protective technologies for stopping the spread of the droplets of aerosol containing the viral particles is a vital requirement to curb the ongoing outbreak.
32798183	7	68	theme	recyclable	1231:1240	arg1	mask					1303:1306	a reusable, recyclable, customizable, antimicrobial and antiviral respirator facial mask	1219:1306	a reusable, recyclable, customizable, antimicrobial and antiviral respirator facial mask feasible for mass production	1219:1335	Here we describe a new design from Egypt, utilizing a reusable, recyclable, customizable, antimicrobial and antiviral respirator facial mask feasible for mass production.
32798183	8	69	theme	acetate	1455:1461	arg1	matrix					1415:1420	a nanofibrous matrix	1401:1420	a nanofibrous matrix of polylactic acid and cellulose acetate containing copper oxide nanoparticles and graphene oxide nanosheets and produced using the electrospinning technique	1401:1578	The novel design is based on the filtration system composed of a nanofibrous matrix of polylactic acid and cellulose acetate containing copper oxide nanoparticles and graphene oxide nanosheets and produced using the electrospinning technique.
32798183	1	70	theme	syndrome	207:214	arg1	coronavirus					216:226	severe acute respiratory syndrome coronavirus 2	182:228	severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)	182:241	The outbreak of the coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is thought to have occurred first in Wuhan, China in December 2019, before spreading to over 120 countries in the months that followed.
32798183	1	70	theme	syndrome	207:214	arg1	SARS-CoV-2					231:240	SARS-CoV-2	231:240	SARS-CoV-2	231:240	The outbreak of the coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is thought to have occurred first in Wuhan, China in December 2019, before spreading to over 120 countries in the months that followed.
32798183	2	71	theme	concern	439:445	arg1	emergency					412:420	a "public health emergency	395:420	a "public health emergency of international concern	395:445	It was declared a "public health emergency of international concern" by the World Health Organization on January 31, 2020 and recognized as a pandemic on March 11, 2020.
32798183	10	72	theme	developing	1765:1774	arg1	setting					1776:1782	a developing setting to provide innovative solutions for combating the SARS-CoV-2 pandemic of potentially global significance	1763:1887	a developing setting to provide innovative solutions for combating the SARS-CoV-2 pandemic of potentially global significance	1763:1887	This design illustrates an effort made in a developing setting to provide innovative solutions for combating the SARS-CoV-2 pandemic of potentially global significance.
32798183	7	73	theme	new	1186:1188	arg1	design					1190:1195	a new design	1184:1195	a new design	1184:1195	Here we describe a new design from Egypt, utilizing a reusable, recyclable, customizable, antimicrobial and antiviral respirator facial mask feasible for mass production.
32798183	5	74	from	generations	850:860	arg1	use					896:898	use	896:898	use	896:898	However, the current generations of protective respirator masks in use are noted for their imperfect design and there is a need to develop their more advanced analogues, with higher blockage efficiency and the ability to deactivate the trapped bacteria and viruses.
32798183	8	75	theme	graphene	1505:1512	arg1	nanosheets					1520:1529	graphene oxide nanosheets	1505:1529	graphene oxide nanosheets	1505:1529	The novel design is based on the filtration system composed of a nanofibrous matrix of polylactic acid and cellulose acetate containing copper oxide nanoparticles and graphene oxide nanosheets and produced using the electrospinning technique.
32798183	2	76	theme	international	425:437	arg1	concern					439:445	international concern	425:445	international concern	425:445	It was declared a "public health emergency of international concern" by the World Health Organization on January 31, 2020 and recognized as a pandemic on March 11, 2020.
32798183	5	77	theme	blockage	1011:1018	arg1	efficiency					1020:1029	higher blockage efficiency	1004:1029	higher blockage efficiency	1004:1029	However, the current generations of protective respirator masks in use are noted for their imperfect design and there is a need to develop their more advanced analogues, with higher blockage efficiency and the ability to deactivate the trapped bacteria and viruses.
32798183	0	78	dep	Protecting	0:9	arg1	protocol					78:85	A protocol	76:85	A protocol for a new device from Egypt	76:113	Protecting healthcare workers during COVID-19 pandemic with nanotechnology: A protocol for a new device from Egypt.
32798183	0	79	with	pandemic	46:53	arg1	nanotechnology					60:73	nanotechnology	60:73	nanotechnology	60:73	Protecting healthcare workers during COVID-19 pandemic with nanotechnology: A protocol for a new device from Egypt.
32798183	7	80	theme	antimicrobial	1257:1269	arg1	mask					1303:1306	a reusable, recyclable, customizable, antimicrobial and antiviral respirator facial mask	1219:1306	a reusable, recyclable, customizable, antimicrobial and antiviral respirator facial mask feasible for mass production	1219:1335	Here we describe a new design from Egypt, utilizing a reusable, recyclable, customizable, antimicrobial and antiviral respirator facial mask feasible for mass production.
32798183	0	81	from	Egypt	109:113	arg1	device					97:102	a new device	91:102	a new device from Egypt	91:113	Protecting healthcare workers during COVID-19 pandemic with nanotechnology: A protocol for a new device from Egypt.
34259622	6	0	theme	overall	779:785	arg1	increase					787:794	an overall increase	776:794	an overall increase in health	776:804	The data indicated an overall increase in health and economic gain for the producer with the addition of MRF to the diet of the broilers.
34259622	2	1	theme	extensive	271:279	arg1	use					281:283	the extensive use	267:283	the extensive use of antibiotics	267:298	The rapid increase in resistance rates has been attributed to the extensive use of antibiotics since they became commercially available.
34259622	5	2	from	timepoints	693:702	arg1	caecum					646:651	the caecum	642:651	the caecum of commercial broilers at two different timepoints within the growth of the broiler, day 27 and day 34	642:754	We investigated the effect of MRF on the microbial community, resistome and metabolic pathways within the caecum of commercial broilers at two different timepoints within the growth of the broiler, day 27 and day 34.
34259622	5	2	from	timepoints	693:702	arg1	broilers					667:674	commercial broilers	656:674	commercial broilers at two different timepoints within the growth of the broiler, day 27 and day 34	656:754	We investigated the effect of MRF on the microbial community, resistome and metabolic pathways within the caecum of commercial broilers at two different timepoints within the growth of the broiler, day 27 and day 34.
34259622	8	3	from	variation	1169:1177	arg1	richness					1216:1223	the antimicrobial resistance gene richness	1182:1223	the antimicrobial resistance gene richness	1182:1223	While all samples harboured resistance genes conferring resistance to the same classes of antibiotics, there was significant variation in the antimicrobial resistance gene richness across time and treatment and across combinations of time and treatment.
34259622	8	4	theme	antimicrobial	1186:1198	arg1	richness					1216:1223	the antimicrobial resistance gene richness	1182:1223	the antimicrobial resistance gene richness	1182:1223	While all samples harboured resistance genes conferring resistance to the same classes of antibiotics, there was significant variation in the antimicrobial resistance gene richness across time and treatment and across combinations of time and treatment.
34259622	13	5	theme	resistome	1785:1793	arg1	variations					1795:1804	The resistome variations	1781:1804	The resistome variations across samples	1781:1819	The resistome variations across samples were correlated with specific genera.
34259622	9	6	with	taxa	1302:1305	arg1	correlation					1321:1331	positive correlation	1312:1331	positive correlation	1312:1331	The taxa with positive correlation comprised Bacilli and Clostridia.
34259622	1	7	theme	threats	170:176	arg1	resistance					120:129	Antibiotic resistance	109:129	Antibiotic resistance	109:129	Antibiotic resistance is regarded as one of the most serious threats to human health worldwide.
34259622	1	7	theme	threats	170:176	arg1	threats					170:176	the most serious threats	153:176	the most serious threats to human health worldwide	153:202	Antibiotic resistance is regarded as one of the most serious threats to human health worldwide.
34259622	1	7	theme	threats	170:176	arg1	one					146:148	one	146:148	one	146:148	Antibiotic resistance is regarded as one of the most serious threats to human health worldwide.
34259622	8	8	theme	antibiotics	1134:1144	arg1	classes					1123:1129	the same classes	1114:1129	the same classes of antibiotics	1114:1144	While all samples harboured resistance genes conferring resistance to the same classes of antibiotics, there was significant variation in the antimicrobial resistance gene richness across time and treatment and across combinations of time and treatment.
34259622	8	9	theme	gene	1211:1214	arg1	richness					1216:1223	the antimicrobial resistance gene richness	1182:1223	the antimicrobial resistance gene richness	1182:1223	While all samples harboured resistance genes conferring resistance to the same classes of antibiotics, there was significant variation in the antimicrobial resistance gene richness across time and treatment and across combinations of time and treatment.
34259622	11	10	theme	pathway	1545:1551	arg1	abundances					1553:1562	the metabolism pathway abundances	1530:1562	the metabolism pathway abundances of the caecal microflora	1530:1587	The KEGG-pathway analysis identified an age-related change in the metabolism pathway abundances of the caecal microflora.
34259622	1	11	theme	human	181:185	arg1	worldwide					194:202	human health worldwide	181:202	human health worldwide	181:202	Antibiotic resistance is regarded as one of the most serious threats to human health worldwide.
34259622	7	12	theme	communities	1012:1022	arg1	richness					986:993	the richness	982:993	the richness of the microbial communities across all samples	982:1041	The only significant difference across the microbial composition of the samples was in the richness of the microbial communities across all samples.
34259622	8	13	theme	significant	1157:1167	arg1	variation					1169:1177	significant variation	1157:1177	significant variation in the antimicrobial resistance gene richness across time and treatment and across combinations of time and treatment	1157:1295	While all samples harboured resistance genes conferring resistance to the same classes of antibiotics, there was significant variation in the antimicrobial resistance gene richness across time and treatment and across combinations of time and treatment.
34259622	6	14	from	increase	787:794	arg1	health					799:804	health	799:804	health	799:804	The data indicated an overall increase in health and economic gain for the producer with the addition of MRF to the diet of the broilers.
34259622	12	15	from	changes	1697:1703	arg1	metabolism					1712:1721	the metabolism	1708:1721	the metabolism of the microbiomes rather than the microbial composition	1708:1778	We suggest that the MRF-related increases in health and weight gain in the broilers may be associated with changes in the metabolism of the microbiomes rather than the microbial composition.
34259622	12	15	from	changes	1697:1703	arg1	composition					1768:1778	the microbial composition	1754:1778	the metabolism of the microbiomes rather than the microbial composition	1708:1778	We suggest that the MRF-related increases in health and weight gain in the broilers may be associated with changes in the metabolism of the microbiomes rather than the microbial composition.
34259622	5	16	theme	broilers	667:674	arg1	caecum					646:651	the caecum	642:651	the caecum of commercial broilers at two different timepoints within the growth of the broiler, day 27 and day 34	642:754	We investigated the effect of MRF on the microbial community, resistome and metabolic pathways within the caecum of commercial broilers at two different timepoints within the growth of the broiler, day 27 and day 34.
34259622	2	17	theme	rapid	209:213	arg1	increase					215:222	The rapid increase	205:222	The rapid increase in resistance rates	205:242	The rapid increase in resistance rates has been attributed to the extensive use of antibiotics since they became commercially available.
34259622	11	18	theme	KEGG-pathway	1472:1483	arg1	analysis					1485:1492	The KEGG-pathway analysis	1468:1492	The KEGG-pathway analysis	1468:1492	The KEGG-pathway analysis identified an age-related change in the metabolism pathway abundances of the caecal microflora.
34259622	15	19	theme	ARGs	2166:2169	arg1	presence					2154:2161	the presence	2150:2161	the presence of ARGs	2150:2169	While the ARGs of greatest concern to human or animal health were not detected in this study, it has identified the potential to reduce the presence of ARGs by the increase in specific genera.
34259622	8	20	theme	time	1278:1281	arg1	combinations					1262:1273	combinations	1262:1273	combinations of time and treatment	1262:1295	While all samples harboured resistance genes conferring resistance to the same classes of antibiotics, there was significant variation in the antimicrobial resistance gene richness across time and treatment and across combinations of time and treatment.
34259622	8	21	theme	treatment	1287:1295	arg1	combinations					1262:1273	combinations	1262:1273	combinations of time and treatment	1262:1295	While all samples harboured resistance genes conferring resistance to the same classes of antibiotics, there was significant variation in the antimicrobial resistance gene richness across time and treatment and across combinations of time and treatment.
34259622	11	22	theme	age-related	1508:1518	arg1	change					1520:1525	an age-related change	1505:1525	an age-related change in the metabolism pathway abundances of the caecal microflora	1505:1587	The KEGG-pathway analysis identified an age-related change in the metabolism pathway abundances of the caecal microflora.
34259622	1	23	theme	Antibiotic	109:118	arg1	resistance					120:129	Antibiotic resistance	109:129	Antibiotic resistance	109:129	Antibiotic resistance is regarded as one of the most serious threats to human health worldwide.
34259622	1	23	theme	Antibiotic	109:118	arg1	threats					170:176	the most serious threats	153:176	the most serious threats to human health worldwide	153:202	Antibiotic resistance is regarded as one of the most serious threats to human health worldwide.
34259622	1	23	theme	Antibiotic	109:118	arg1	one					146:148	one	146:148	one	146:148	Antibiotic resistance is regarded as one of the most serious threats to human health worldwide.
34259622	0	24	theme	broiler	91:97	arg1	chickens					99:106	broiler chickens	91:106	broiler chickens	91:106	Investigation into the effect of mannan-rich fraction supplementation on the metagenome of broiler chickens.
34259622	15	25	theme	human	2052:2056	arg1	health					2068:2073	human or animal health	2052:2073	human or animal health	2052:2073	While the ARGs of greatest concern to human or animal health were not detected in this study, it has identified the potential to reduce the presence of ARGs by the increase in specific genera.
34259622	2	26	from	increase	215:222	arg1	rates					238:242	resistance rates	227:242	resistance rates	227:242	The rapid increase in resistance rates has been attributed to the extensive use of antibiotics since they became commercially available.
34259622	10	27	theme	Streptococcus	1446:1458	arg1	genera					1460:1465	the Streptococcus genera	1442:1465	the Streptococcus genera	1442:1465	The negative correlation taxa were also dominated by Bacilli, specifically the Streptococcus genera.
34259622	14	28	used	used	1877:1880	arg2	data					1865:1868	These data	1859:1868	These data	1859:1868	These data may be used to further enhance the development of feed supplements to reduce the presence of antibiotic resistance genes (ARGs) within poultry.
34259622	5	29	theme	broiler	729:735	arg1	growth					715:720	the growth	711:720	the growth of the broiler, day 27 and day 34	711:754	We investigated the effect of MRF on the microbial community, resistome and metabolic pathways within the caecum of commercial broilers at two different timepoints within the growth of the broiler, day 27 and day 34.
34259622	15	30	theme	concern	2041:2047	arg1	ARGs					2024:2027	the ARGs	2020:2027	the ARGs of greatest concern to human or animal health	2020:2073	While the ARGs of greatest concern to human or animal health were not detected in this study, it has identified the potential to reduce the presence of ARGs by the increase in specific genera.
34259622	5	31	theme	MRF	570:572	arg1	effect					560:565	the effect	556:565	the effect of MRF on the microbial community, resistome and metabolic pathways within the caecum of commercial broilers at two different timepoints within the growth of the broiler, day 27 and day 34	556:754	We investigated the effect of MRF on the microbial community, resistome and metabolic pathways within the caecum of commercial broilers at two different timepoints within the growth of the broiler, day 27 and day 34.
34259622	15	32	from	increase	2178:2185	arg1	genera					2199:2204	specific genera	2190:2204	specific genera	2190:2204	While the ARGs of greatest concern to human or animal health were not detected in this study, it has identified the potential to reduce the presence of ARGs by the increase in specific genera.
34259622	14	33	theme	resistance	1974:1983	arg1	ARGs					1992:1995	ARGs	1992:1995	ARGs	1992:1995	These data may be used to further enhance the development of feed supplements to reduce the presence of antibiotic resistance genes (ARGs) within poultry.
34259622	14	33	theme	resistance	1974:1983	arg1	genes					1985:1989	antibiotic resistance genes	1963:1989	antibiotic resistance genes (ARGs)	1963:1996	These data may be used to further enhance the development of feed supplements to reduce the presence of antibiotic resistance genes (ARGs) within poultry.
34259622	9	34	theme	positive	1312:1319	arg1	correlation					1321:1331	positive correlation	1312:1331	positive correlation	1312:1331	The taxa with positive correlation comprised Bacilli and Clostridia.
34259622	5	35	from	caecum	646:651	arg1	timepoints					693:702	two different timepoints	679:702	two different timepoints within the growth of the broiler, day 27 and day 34	679:754	We investigated the effect of MRF on the microbial community, resistome and metabolic pathways within the caecum of commercial broilers at two different timepoints within the growth of the broiler, day 27 and day 34.
34259622	0	36	theme	fraction	45:52	arg1	supplementation					54:68	mannan-rich fraction supplementation	33:68	mannan-rich fraction supplementation	33:68	Investigation into the effect of mannan-rich fraction supplementation on the metagenome of broiler chickens.
34259622	11	37	theme	microflora	1578:1587	arg1	abundances					1553:1562	the metabolism pathway abundances	1530:1562	the metabolism pathway abundances of the caecal microflora	1530:1587	The KEGG-pathway analysis identified an age-related change in the metabolism pathway abundances of the caecal microflora.
34259622	8	38	theme	resistance	1072:1081	arg1	genes					1083:1087	resistance genes	1072:1087	resistance genes conferring resistance to the same classes of antibiotics	1072:1144	While all samples harboured resistance genes conferring resistance to the same classes of antibiotics, there was significant variation in the antimicrobial resistance gene richness across time and treatment and across combinations of time and treatment.
34259622	15	39	theme	animal	2061:2066	arg1	health					2068:2073	human or animal health	2052:2073	human or animal health	2052:2073	While the ARGs of greatest concern to human or animal health were not detected in this study, it has identified the potential to reduce the presence of ARGs by the increase in specific genera.
34259622	4	40	theme	growth-promoting	499:514	arg1	effects					516:522	similar growth-promoting effects	491:522	similar growth-promoting effects	491:522	Mannan-rich fraction (MRF) has been reported to show similar growth-promoting effects to antibiotics.
34259622	6	41	theme	MRF	862:864	arg1	addition					850:857	the addition	846:857	the addition of MRF to the diet of the broilers	846:892	The data indicated an overall increase in health and economic gain for the producer with the addition of MRF to the diet of the broilers.
34259622	11	42	theme	metabolism	1534:1543	arg1	abundances					1553:1562	the metabolism pathway abundances	1530:1562	the metabolism pathway abundances of the caecal microflora	1530:1587	The KEGG-pathway analysis identified an age-related change in the metabolism pathway abundances of the caecal microflora.
34259622	14	43	theme	feed	1920:1923	arg1	supplements					1925:1935	feed supplements	1920:1935	feed supplements	1920:1935	These data may be used to further enhance the development of feed supplements to reduce the presence of antibiotic resistance genes (ARGs) within poultry.
34259622	12	44	theme	microbial	1758:1766	arg1	composition					1768:1778	the microbial composition	1754:1778	the metabolism of the microbiomes rather than the microbial composition	1708:1778	We suggest that the MRF-related increases in health and weight gain in the broilers may be associated with changes in the metabolism of the microbiomes rather than the microbial composition.
34259622	1	45	theme	serious	162:168	arg1	threats					170:176	the most serious threats	153:176	the most serious threats to human health worldwide	153:202	Antibiotic resistance is regarded as one of the most serious threats to human health worldwide.
34259622	8	46	theme	same	1118:1121	arg1	classes					1123:1129	the same classes	1114:1129	the same classes of antibiotics	1114:1144	While all samples harboured resistance genes conferring resistance to the same classes of antibiotics, there was significant variation in the antimicrobial resistance gene richness across time and treatment and across combinations of time and treatment.
34259622	6	47	from	gain	819:822	arg1	health					799:804	health	799:804	health	799:804	The data indicated an overall increase in health and economic gain for the producer with the addition of MRF to the diet of the broilers.
34259622	5	48	theme	metabolic	616:624	arg1	pathways					626:633	metabolic pathways	616:633	metabolic pathways	616:633	We investigated the effect of MRF on the microbial community, resistome and metabolic pathways within the caecum of commercial broilers at two different timepoints within the growth of the broiler, day 27 and day 34.
34259622	5	48	theme	metabolic	616:624	arg1	community					591:599	the microbial community	577:599	the microbial community	577:599	We investigated the effect of MRF on the microbial community, resistome and metabolic pathways within the caecum of commercial broilers at two different timepoints within the growth of the broiler, day 27 and day 34.
34259622	12	49	theme	microbiomes	1730:1740	arg1	metabolism					1712:1721	the metabolism	1708:1721	the metabolism of the microbiomes rather than the microbial composition	1708:1778	We suggest that the MRF-related increases in health and weight gain in the broilers may be associated with changes in the metabolism of the microbiomes rather than the microbial composition.
34259622	8	50	theme	resistance	1200:1209	arg1	richness					1216:1223	the antimicrobial resistance gene richness	1182:1223	the antimicrobial resistance gene richness	1182:1223	While all samples harboured resistance genes conferring resistance to the same classes of antibiotics, there was significant variation in the antimicrobial resistance gene richness across time and treatment and across combinations of time and treatment.
34259622	10	51	theme	correlation	1380:1390	arg1	taxa					1392:1395	The negative correlation taxa	1367:1395	The negative correlation taxa	1367:1395	The negative correlation taxa were also dominated by Bacilli, specifically the Streptococcus genera.
34259622	7	52	theme	microbial	1002:1010	arg1	communities					1012:1022	the microbial communities	998:1022	the microbial communities	998:1022	The only significant difference across the microbial composition of the samples was in the richness of the microbial communities across all samples.
34259622	10	53	theme	negative	1371:1378	arg1	taxa					1392:1395	The negative correlation taxa	1367:1395	The negative correlation taxa	1367:1395	The negative correlation taxa were also dominated by Bacilli, specifically the Streptococcus genera.
34259622	2	54	theme	antibiotics	288:298	arg1	use					281:283	the extensive use	267:283	the extensive use of antibiotics	267:298	The rapid increase in resistance rates has been attributed to the extensive use of antibiotics since they became commercially available.
34259622	1	55	theme	health	187:192	arg1	worldwide					194:202	human health worldwide	181:202	human health worldwide	181:202	Antibiotic resistance is regarded as one of the most serious threats to human health worldwide.
34259622	12	56	theme	MRF-related	1610:1620	arg1	increases					1622:1630	the MRF-related increases	1606:1630	the MRF-related increases in health and weight gain in the broilers	1606:1672	We suggest that the MRF-related increases in health and weight gain in the broilers may be associated with changes in the metabolism of the microbiomes rather than the microbial composition.
34259622	4	57	theme	Mannan-rich	438:448	arg1	MRF					460:462	MRF	460:462	MRF	460:462	Mannan-rich fraction (MRF) has been reported to show similar growth-promoting effects to antibiotics.
34259622	4	57	theme	Mannan-rich	438:448	arg1	fraction					450:457	Mannan-rich fraction	438:457	Mannan-rich fraction (MRF)	438:463	Mannan-rich fraction (MRF) has been reported to show similar growth-promoting effects to antibiotics.
34259622	12	58	from	increases	1622:1630	arg1	health					1635:1640	health	1635:1640	health	1635:1640	We suggest that the MRF-related increases in health and weight gain in the broilers may be associated with changes in the metabolism of the microbiomes rather than the microbial composition.
34259622	12	58	from	increases	1622:1630	arg1	gain					1653:1656	weight gain	1646:1656	weight gain	1646:1656	We suggest that the MRF-related increases in health and weight gain in the broilers may be associated with changes in the metabolism of the microbiomes rather than the microbial composition.
34259622	12	58	from	increases	1622:1630	arg1	broilers					1665:1672	the broilers	1661:1672	the broilers	1661:1672	We suggest that the MRF-related increases in health and weight gain in the broilers may be associated with changes in the metabolism of the microbiomes rather than the microbial composition.
34259622	2	59	theme	resistance	227:236	arg1	rates					238:242	resistance rates	227:242	resistance rates	227:242	The rapid increase in resistance rates has been attributed to the extensive use of antibiotics since they became commercially available.
34259622	6	60	theme	broilers	885:892	arg1	diet					873:876	the diet	869:876	the diet of the broilers	869:892	The data indicated an overall increase in health and economic gain for the producer with the addition of MRF to the diet of the broilers.
34259622	5	61	theme	commercial	656:665	arg1	broilers					667:674	commercial broilers	656:674	commercial broilers at two different timepoints within the growth of the broiler, day 27 and day 34	656:754	We investigated the effect of MRF on the microbial community, resistome and metabolic pathways within the caecum of commercial broilers at two different timepoints within the growth of the broiler, day 27 and day 34.
34259622	7	62	theme	samples	967:973	arg1	composition					948:958	the microbial composition	934:958	the microbial composition of the samples	934:973	The only significant difference across the microbial composition of the samples was in the richness of the microbial communities across all samples.
34259622	5	63	theme	different	683:691	arg1	timepoints					693:702	two different timepoints	679:702	two different timepoints within the growth of the broiler, day 27 and day 34	679:754	We investigated the effect of MRF on the microbial community, resistome and metabolic pathways within the caecum of commercial broilers at two different timepoints within the growth of the broiler, day 27 and day 34.
34259622	7	64	theme	significant	904:914	arg1	difference					916:925	The only significant difference	895:925	The only significant difference across the microbial composition of the samples	895:973	The only significant difference across the microbial composition of the samples was in the richness of the microbial communities across all samples.
34259622	5	65	from	effect	560:565	arg1	resistome					602:610	resistome	602:610	resistome	602:610	We investigated the effect of MRF on the microbial community, resistome and metabolic pathways within the caecum of commercial broilers at two different timepoints within the growth of the broiler, day 27 and day 34.
34259622	5	65	from	effect	560:565	arg1	pathways					626:633	metabolic pathways	616:633	metabolic pathways	616:633	We investigated the effect of MRF on the microbial community, resistome and metabolic pathways within the caecum of commercial broilers at two different timepoints within the growth of the broiler, day 27 and day 34.
34259622	5	65	from	effect	560:565	arg1	community					591:599	the microbial community	577:599	the microbial community	577:599	We investigated the effect of MRF on the microbial community, resistome and metabolic pathways within the caecum of commercial broilers at two different timepoints within the growth of the broiler, day 27 and day 34.
34259622	7	66	theme	microbial	938:946	arg1	composition					948:958	the microbial composition	934:958	the microbial composition of the samples	934:973	The only significant difference across the microbial composition of the samples was in the richness of the microbial communities across all samples.
34259622	11	67	from	change	1520:1525	arg1	abundances					1553:1562	the metabolism pathway abundances	1530:1562	the metabolism pathway abundances of the caecal microflora	1530:1587	The KEGG-pathway analysis identified an age-related change in the metabolism pathway abundances of the caecal microflora.
34259622	0	68	from	effect	23:28	arg1	metagenome					77:86	the metagenome	73:86	the metagenome of broiler chickens	73:106	Investigation into the effect of mannan-rich fraction supplementation on the metagenome of broiler chickens.
34259622	4	69	theme	similar	491:497	arg1	effects					516:522	similar growth-promoting effects	491:522	similar growth-promoting effects	491:522	Mannan-rich fraction (MRF) has been reported to show similar growth-promoting effects to antibiotics.
34259622	0	70	theme	chickens	99:106	arg1	metagenome					77:86	the metagenome	73:86	the metagenome of broiler chickens	73:106	Investigation into the effect of mannan-rich fraction supplementation on the metagenome of broiler chickens.
34259622	5	71	theme	microbial	581:589	arg1	resistome					602:610	resistome	602:610	resistome	602:610	We investigated the effect of MRF on the microbial community, resistome and metabolic pathways within the caecum of commercial broilers at two different timepoints within the growth of the broiler, day 27 and day 34.
34259622	5	71	theme	microbial	581:589	arg1	pathways					626:633	metabolic pathways	616:633	metabolic pathways	616:633	We investigated the effect of MRF on the microbial community, resistome and metabolic pathways within the caecum of commercial broilers at two different timepoints within the growth of the broiler, day 27 and day 34.
34259622	5	71	theme	microbial	581:589	arg1	community					591:599	the microbial community	577:599	the microbial community	577:599	We investigated the effect of MRF on the microbial community, resistome and metabolic pathways within the caecum of commercial broilers at two different timepoints within the growth of the broiler, day 27 and day 34.
34259622	15	72	located	detected	2084:2091	arg1	study					2101:2105	this study	2096:2105	this study	2096:2105	While the ARGs of greatest concern to human or animal health were not detected in this study, it has identified the potential to reduce the presence of ARGs by the increase in specific genera.
34259622	15	72	located	detected	2084:2091	arg2	ARGs					2024:2027	the ARGs	2020:2027	the ARGs of greatest concern to human or animal health	2020:2073	While the ARGs of greatest concern to human or animal health were not detected in this study, it has identified the potential to reduce the presence of ARGs by the increase in specific genera.
34259622	3	73	theme	antibiotics	353:363	arg1	use					346:348	The use	342:348	The use of antibiotics as growth promotors	342:383	The use of antibiotics as growth promotors has been banned in numerous regions for this reason.
34259622	15	74	theme	greatest	2032:2039	arg1	concern					2041:2047	greatest concern	2032:2047	greatest concern to human or animal health	2032:2073	While the ARGs of greatest concern to human or animal health were not detected in this study, it has identified the potential to reduce the presence of ARGs by the increase in specific genera.
34259622	12	75	theme	weight	1646:1651	arg1	gain					1653:1656	weight gain	1646:1656	weight gain	1646:1656	We suggest that the MRF-related increases in health and weight gain in the broilers may be associated with changes in the metabolism of the microbiomes rather than the microbial composition.
34259622	14	76	theme	genes	1985:1989	arg1	presence					1951:1958	the presence	1947:1958	the presence of antibiotic resistance genes (ARGs) within poultry	1947:2011	These data may be used to further enhance the development of feed supplements to reduce the presence of antibiotic resistance genes (ARGs) within poultry.
34259622	3	77	theme	growth	368:373	arg1	promotors					375:383	growth promotors	368:383	growth promotors	368:383	The use of antibiotics as growth promotors has been banned in numerous regions for this reason.
34259622	13	78	theme	specific	1842:1849	arg1	genera					1851:1856	specific genera	1842:1856	specific genera	1842:1856	The resistome variations across samples were correlated with specific genera.
34259622	0	79	theme	mannan-rich	33:43	arg1	supplementation					54:68	mannan-rich fraction supplementation	33:68	mannan-rich fraction supplementation	33:68	Investigation into the effect of mannan-rich fraction supplementation on the metagenome of broiler chickens.
34259622	14	80	theme	antibiotic	1963:1972	arg1	ARGs					1992:1995	ARGs	1992:1995	ARGs	1992:1995	These data may be used to further enhance the development of feed supplements to reduce the presence of antibiotic resistance genes (ARGs) within poultry.
34259622	14	80	theme	antibiotic	1963:1972	arg1	genes					1985:1989	antibiotic resistance genes	1963:1989	antibiotic resistance genes (ARGs)	1963:1996	These data may be used to further enhance the development of feed supplements to reduce the presence of antibiotic resistance genes (ARGs) within poultry.
34259622	3	81	theme	numerous	404:411	arg1	regions					413:419	numerous regions	404:419	numerous regions for this reason	404:435	The use of antibiotics as growth promotors has been banned in numerous regions for this reason.
34259622	0	82	theme	supplementation	54:68	arg1	effect					23:28	the effect	19:28	the effect of mannan-rich fraction supplementation on the metagenome of broiler chickens	19:106	Investigation into the effect of mannan-rich fraction supplementation on the metagenome of broiler chickens.
34259622	6	83	theme	economic	810:817	arg1	gain					819:822	economic gain	810:822	economic gain for the producer	810:839	The data indicated an overall increase in health and economic gain for the producer with the addition of MRF to the diet of the broilers.
34259622	11	84	theme	caecal	1571:1576	arg1	microflora					1578:1587	the caecal microflora	1567:1587	the caecal microflora	1567:1587	The KEGG-pathway analysis identified an age-related change in the metabolism pathway abundances of the caecal microflora.
34259622	15	85	theme	specific	2190:2197	arg1	genera					2199:2204	specific genera	2190:2204	specific genera	2190:2204	While the ARGs of greatest concern to human or animal health were not detected in this study, it has identified the potential to reduce the presence of ARGs by the increase in specific genera.
34259622	14	86	theme	supplements	1925:1935	arg1	development					1905:1915	the development	1901:1915	the development of feed supplements	1901:1935	These data may be used to further enhance the development of feed supplements to reduce the presence of antibiotic resistance genes (ARGs) within poultry.
35011480	1	0	theme	scanning	366:373	arg1	spectroscopy					416:427	scanning electron microscopy-electron diffraction spectroscopy	366:427	scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS)	366:437	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	0	theme	scanning	366:373	arg1	techniques					329:338	various analytical techniques	310:338	various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM))	310:481	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	0	theme	scanning	366:373	arg1	SEM-EDS					430:436	SEM-EDS	430:436	SEM-EDS	430:436	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	0	1	theme	Chromatographic	184:198	arg1	Analysis					200:207	Liquid Chromatographic Analysis	177:207	Liquid Chromatographic Analysis	177:207	Beta-Cyclodextrin-Decorated Magnetic Activated Carbon as a Sorbent for Extraction and Enrichment of Steroid Hormones (Estrone, β-Estradiol, Hydrocortisone and Progesterone) for Liquid Chromatographic Analysis.
35011480	2	2	theme	composite	751:759	arg1	design					761:766	the central composite design in order to determine the experimental parameters affecting the extraction procedure	739:851	the central composite design in order to determine the experimental parameters affecting the extraction procedure	739:851	This method was optimized using the central composite design in order to determine the experimental parameters affecting the extraction procedure.
35011480	1	3	theme	progesterone	655:666	arg1	preconcentration					594:609	the preconcentration	590:609	the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples	590:704	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	4	theme	electron	375:382	arg1	spectroscopy					416:427	scanning electron microscopy-electron diffraction spectroscopy	366:427	scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS)	366:437	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	4	theme	electron	375:382	arg1	techniques					329:338	various analytical techniques	310:338	various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM))	310:481	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	4	theme	electron	375:382	arg1	SEM-EDS					430:436	SEM-EDS	430:436	SEM-EDS	430:436	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	0	5	theme	Liquid	177:182	arg1	Analysis					200:207	Liquid Chromatographic Analysis	177:207	Liquid Chromatographic Analysis	177:207	Beta-Cyclodextrin-Decorated Magnetic Activated Carbon as a Sorbent for Extraction and Enrichment of Steroid Hormones (Estrone, β-Estradiol, Hydrocortisone and Progesterone) for Liquid Chromatographic Analysis.
35011480	4	6	dep	300	1057:1059	arg1	to					1054:1055	to	1054:1055	to	1054:1055	Under optimum conditions, the linearity ranged from 0.04 to 300 µg L-1 with a correlation of determinations of 0.9969-0.9991.
35011480	7	7	from	use	1491:1493	arg1	samples					1509:1515	wastewater samples	1498:1515	wastewater samples without significant interferences	1498:1549	The developed procedure demonstrated high potential as an effective technique for use in wastewater samples without significant interferences, and the adsorbent could be reused up to eight times.
35011480	0	8	theme	Hormones	108:115	arg1	Enrichment					86:95	Enrichment	86:95	Enrichment	86:95	Beta-Cyclodextrin-Decorated Magnetic Activated Carbon as a Sorbent for Extraction and Enrichment of Steroid Hormones (Estrone, β-Estradiol, Hydrocortisone and Progesterone) for Liquid Chromatographic Analysis.
35011480	0	8	theme	Hormones	108:115	arg1	Extraction					71:80	Extraction	71:80	Extraction	71:80	Beta-Cyclodextrin-Decorated Magnetic Activated Carbon as a Sorbent for Extraction and Enrichment of Steroid Hormones (Estrone, β-Estradiol, Hydrocortisone and Progesterone) for Liquid Chromatographic Analysis.
35011480	1	9	theme	microscopy-electron	384:402	arg1	spectroscopy					416:427	scanning electron microscopy-electron diffraction spectroscopy	366:427	scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS)	366:437	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	9	theme	microscopy-electron	384:402	arg1	techniques					329:338	various analytical techniques	310:338	various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM))	310:481	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	9	theme	microscopy-electron	384:402	arg1	SEM-EDS					430:436	SEM-EDS	430:436	SEM-EDS	430:436	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	10	theme	wastewater	671:680	arg1	samples					698:704	wastewater and river water samples	671:704	samples	698:704	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	0	11	theme	Steroid	100:106	arg1	Hormones					108:115	Steroid Hormones	100:115	Steroid Hormones (Estrone, β-Estradiol, Hydrocortisone and Progesterone)	100:171	Beta-Cyclodextrin-Decorated Magnetic Activated Carbon as a Sorbent for Extraction and Enrichment of Steroid Hormones (Estrone, β-Estradiol, Hydrocortisone and Progesterone) for Liquid Chromatographic Analysis.
35011480	0	11	theme	Steroid	100:106	arg1	Hydrocortisone					140:153	Hydrocortisone	140:153	Hydrocortisone	140:153	Beta-Cyclodextrin-Decorated Magnetic Activated Carbon as a Sorbent for Extraction and Enrichment of Steroid Hormones (Estrone, β-Estradiol, Hydrocortisone and Progesterone) for Liquid Chromatographic Analysis.
35011480	0	11	theme	Steroid	100:106	arg1	Progesterone					159:170	Progesterone	159:170	Progesterone	159:170	Beta-Cyclodextrin-Decorated Magnetic Activated Carbon as a Sorbent for Extraction and Enrichment of Steroid Hormones (Estrone, β-Estradiol, Hydrocortisone and Progesterone) for Liquid Chromatographic Analysis.
35011480	0	11	theme	Steroid	100:106	arg1	β-Estradiol					127:137	β-Estradiol	127:137	β-Estradiol	127:137	Beta-Cyclodextrin-Decorated Magnetic Activated Carbon as a Sorbent for Extraction and Enrichment of Steroid Hormones (Estrone, β-Estradiol, Hydrocortisone and Progesterone) for Liquid Chromatographic Analysis.
35011480	0	11	theme	Steroid	100:106	arg1	Estrone					118:124	Estrone	118:124	Estrone	118:124	Beta-Cyclodextrin-Decorated Magnetic Activated Carbon as a Sorbent for Extraction and Enrichment of Steroid Hormones (Estrone, β-Estradiol, Hydrocortisone and Progesterone) for Liquid Chromatographic Analysis.
35011480	1	12	theme	diffraction	404:414	arg1	spectroscopy					416:427	scanning electron microscopy-electron diffraction spectroscopy	366:427	scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS)	366:437	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	12	theme	diffraction	404:414	arg1	techniques					329:338	various analytical techniques	310:338	various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM))	310:481	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	12	theme	diffraction	404:414	arg1	SEM-EDS					430:436	SEM-EDS	430:436	SEM-EDS	430:436	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	13	theme	β-estradiol	623:633	arg1	preconcentration					594:609	the preconcentration	590:609	the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples	590:704	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	6	14	theme	isotherm	1330:1337	arg1	model					1339:1343	the Langmuir isotherm model	1317:1343	the Langmuir isotherm model	1317:1343	The equilibrium data were best described by the Langmuir isotherm model, and high adsorption capacities (217-294 mg g-1) were obtained.
35011480	2	15	theme	experimental	794:805	arg1	parameters					807:816	the experimental parameters	790:816	the experimental parameters affecting the extraction procedure	790:851	This method was optimized using the central composite design in order to determine the experimental parameters affecting the extraction procedure.
35011480	1	16	theme	various	310:316	arg1	microscopy					465:474	transmission electron microscopy	443:474	transmission electron microscopy (TEM)	443:480	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	16	theme	various	310:316	arg1	spectroscopy					416:427	scanning electron microscopy-electron diffraction spectroscopy	366:427	scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS)	366:437	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	16	theme	various	310:316	arg1	techniques					329:338	various analytical techniques	310:338	various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM))	310:481	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	16	theme	various	310:316	arg1	diffraction					347:357	X-ray diffraction	341:357	X-ray diffraction (XRD)	341:363	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	7	17	theme	significant	1525:1535	arg1	interferences					1537:1549	significant interferences	1525:1549	significant interferences	1525:1549	The developed procedure demonstrated high potential as an effective technique for use in wastewater samples without significant interferences, and the adsorbent could be reused up to eight times.
35011480	6	18	theme	Langmuir	1321:1328	arg1	model					1339:1343	the Langmuir isotherm model	1317:1343	the Langmuir isotherm model	1317:1343	The equilibrium data were best described by the Langmuir isotherm model, and high adsorption capacities (217-294 mg g-1) were obtained.
35011480	3	19	theme	photodiode	959:968	arg1	HPLC-DAD					986:993	HPLC-DAD	986:993	HPLC-DAD	986:993	The quantification of hormones was achieved using high-performance liquid chromatography equipped with a photodiode array detector (HPLC-DAD).
35011480	3	19	theme	photodiode	959:968	arg1	detector					976:983	a photodiode array detector	957:983	a photodiode array detector (HPLC-DAD)	957:994	The quantification of hormones was achieved using high-performance liquid chromatography equipped with a photodiode array detector (HPLC-DAD).
35011480	0	20	dep	Hormones	108:115	arg1	Progesterone					159:170	Progesterone	159:170	Progesterone	159:170	Beta-Cyclodextrin-Decorated Magnetic Activated Carbon as a Sorbent for Extraction and Enrichment of Steroid Hormones (Estrone, β-Estradiol, Hydrocortisone and Progesterone) for Liquid Chromatographic Analysis.
35011480	0	20	dep	Hormones	108:115	arg1	Hydrocortisone					140:153	Hydrocortisone	140:153	Hydrocortisone	140:153	Beta-Cyclodextrin-Decorated Magnetic Activated Carbon as a Sorbent for Extraction and Enrichment of Steroid Hormones (Estrone, β-Estradiol, Hydrocortisone and Progesterone) for Liquid Chromatographic Analysis.
35011480	0	20	dep	Hormones	108:115	arg1	β-Estradiol					127:137	β-Estradiol	127:137	β-Estradiol	127:137	Beta-Cyclodextrin-Decorated Magnetic Activated Carbon as a Sorbent for Extraction and Enrichment of Steroid Hormones (Estrone, β-Estradiol, Hydrocortisone and Progesterone) for Liquid Chromatographic Analysis.
35011480	0	20	dep	Hormones	108:115	arg1	Hormones					108:115	Steroid Hormones	100:115	Steroid Hormones (Estrone, β-Estradiol, Hydrocortisone and Progesterone)	100:171	Beta-Cyclodextrin-Decorated Magnetic Activated Carbon as a Sorbent for Extraction and Enrichment of Steroid Hormones (Estrone, β-Estradiol, Hydrocortisone and Progesterone) for Liquid Chromatographic Analysis.
35011480	0	20	dep	Hormones	108:115	arg1	Estrone					118:124	Estrone	118:124	Estrone	118:124	Beta-Cyclodextrin-Decorated Magnetic Activated Carbon as a Sorbent for Extraction and Enrichment of Steroid Hormones (Estrone, β-Estradiol, Hydrocortisone and Progesterone) for Liquid Chromatographic Analysis.
35011480	3	21	theme	hormones	876:883	arg1	quantification					858:871	The quantification	854:871	The quantification of hormones	854:883	The quantification of hormones was achieved using high-performance liquid chromatography equipped with a photodiode array detector (HPLC-DAD).
35011480	7	22	theme	wastewater	1498:1507	arg1	samples					1509:1515	wastewater samples	1498:1515	wastewater samples without significant interferences	1498:1549	The developed procedure demonstrated high potential as an effective technique for use in wastewater samples without significant interferences, and the adsorbent could be reused up to eight times.
35011480	5	23	from	1.1-3.4	1252:1258	arg1	precisions					1238:1247	intraday and interday precisions	1216:1247	intraday and interday precisions at 1.1-3.4 and 3.2-4.2	1216:1270	The limits of detection and quantification were between 0.01-0.03 and 0.033-0.1 µg L-1, with intraday and interday precisions at 1.1-3.4 and 3.2-4.2.
35011480	0	24	theme	Beta-Cyclodextrin-Decorated	0:26	arg1	Carbon					47:52	Beta-Cyclodextrin-Decorated Magnetic Activated Carbon	0:52	Beta-Cyclodextrin-Decorated Magnetic Activated Carbon as a Sorbent for Extraction and Enrichment of Steroid Hormones (Estrone, β-Estradiol, Hydrocortisone and Progesterone) for Liquid Chromatographic Analysis.	0:208	Beta-Cyclodextrin-Decorated Magnetic Activated Carbon as a Sorbent for Extraction and Enrichment of Steroid Hormones (Estrone, β-Estradiol, Hydrocortisone and Progesterone) for Liquid Chromatographic Analysis.
35011480	1	25	theme	method	579:584	arg1	development					518:528	the development	514:528	the development of a magnetic solid-phase microextraction (MSPE) method	514:584	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	0	26	theme	Magnetic	28:35	arg1	Carbon					47:52	Beta-Cyclodextrin-Decorated Magnetic Activated Carbon	0:52	Beta-Cyclodextrin-Decorated Magnetic Activated Carbon as a Sorbent for Extraction and Enrichment of Steroid Hormones (Estrone, β-Estradiol, Hydrocortisone and Progesterone) for Liquid Chromatographic Analysis.	0:208	Beta-Cyclodextrin-Decorated Magnetic Activated Carbon as a Sorbent for Extraction and Enrichment of Steroid Hormones (Estrone, β-Estradiol, Hydrocortisone and Progesterone) for Liquid Chromatographic Analysis.
35011480	5	27	theme	interday	1229:1236	arg1	precisions					1238:1247	intraday and interday precisions	1216:1247	intraday and interday precisions at 1.1-3.4 and 3.2-4.2	1216:1270	The limits of detection and quantification were between 0.01-0.03 and 0.033-0.1 µg L-1, with intraday and interday precisions at 1.1-3.4 and 3.2-4.2.
35011480	6	28	theme	high	1350:1353	arg1	capacities					1366:1375	high adsorption capacities	1350:1375	high adsorption capacities (217-294 mg g-1)	1350:1392	The equilibrium data were best described by the Langmuir isotherm model, and high adsorption capacities (217-294 mg g-1) were obtained.
35011480	6	28	theme	high	1350:1353	arg1	g-1					1389:1391	217-294 mg g-1	1378:1391	217-294 mg g-1	1378:1391	The equilibrium data were best described by the Langmuir isotherm model, and high adsorption capacities (217-294 mg g-1) were obtained.
35011480	1	29	theme	river	686:690	arg1	samples					698:704	wastewater and river water samples	671:704	samples	698:704	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	30	theme	analytical	318:327	arg1	microscopy					465:474	transmission electron microscopy	443:474	transmission electron microscopy (TEM)	443:480	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	30	theme	analytical	318:327	arg1	spectroscopy					416:427	scanning electron microscopy-electron diffraction spectroscopy	366:427	scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS)	366:437	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	30	theme	analytical	318:327	arg1	techniques					329:338	various analytical techniques	310:338	various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM))	310:481	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	30	theme	analytical	318:327	arg1	diffraction					347:357	X-ray diffraction	341:357	X-ray diffraction (XRD)	341:363	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	3	31	theme	high-performance	904:919	arg1	chromatography					928:941	high-performance liquid chromatography	904:941	high-performance liquid chromatography equipped with a photodiode array detector (HPLC-DAD)	904:994	The quantification of hormones was achieved using high-performance liquid chromatography equipped with a photodiode array detector (HPLC-DAD).
35011480	7	32	theme	effective	1467:1475	arg1	potential					1451:1459	high potential	1446:1459	high potential	1446:1459	The developed procedure demonstrated high potential as an effective technique for use in wastewater samples without significant interferences, and the adsorbent could be reused up to eight times.
35011480	7	32	theme	effective	1467:1475	arg1	technique					1477:1485	an effective technique	1464:1485	an effective technique for use in wastewater samples without significant interferences	1464:1549	The developed procedure demonstrated high potential as an effective technique for use in wastewater samples without significant interferences, and the adsorbent could be reused up to eight times.
35011480	2	33	theme	extraction	832:841	arg1	procedure					843:851	the extraction procedure	828:851	the extraction procedure	828:851	This method was optimized using the central composite design in order to determine the experimental parameters affecting the extraction procedure.
35011480	1	34	theme	water	692:696	arg1	samples					698:704	wastewater and river water samples	671:704	samples	698:704	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	6	35	theme	equilibrium	1277:1287	arg1	data					1289:1292	The equilibrium data	1273:1292	The equilibrium data	1273:1292	The equilibrium data were best described by the Langmuir isotherm model, and high adsorption capacities (217-294 mg g-1) were obtained.
35011480	0	36	theme	Activated	37:45	arg1	Carbon					47:52	Beta-Cyclodextrin-Decorated Magnetic Activated Carbon	0:52	Beta-Cyclodextrin-Decorated Magnetic Activated Carbon as a Sorbent for Extraction and Enrichment of Steroid Hormones (Estrone, β-Estradiol, Hydrocortisone and Progesterone) for Liquid Chromatographic Analysis.	0:208	Beta-Cyclodextrin-Decorated Magnetic Activated Carbon as a Sorbent for Extraction and Enrichment of Steroid Hormones (Estrone, β-Estradiol, Hydrocortisone and Progesterone) for Liquid Chromatographic Analysis.
35011480	1	37	theme	β-cyclodextrin-decorated	212:235	arg1	carbon					256:261	A β-cyclodextrin-decorated magnetic activated carbon	210:261	A β-cyclodextrin-decorated magnetic activated carbon adsorbent	210:271	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	4	38	theme	µg	1061:1062	arg1	L-1					1064:1066	0.04 to 300 µg L-1	1049:1066	0.04 to 300 µg L-1	1049:1066	Under optimum conditions, the linearity ranged from 0.04 to 300 µg L-1 with a correlation of determinations of 0.9969-0.9991.
35011480	1	39	theme	X-ray	341:345	arg1	XRD					360:362	XRD	360:362	XRD	360:362	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	39	theme	X-ray	341:345	arg1	techniques					329:338	various analytical techniques	310:338	various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM))	310:481	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	39	theme	X-ray	341:345	arg1	diffraction					347:357	X-ray diffraction	341:357	X-ray diffraction (XRD)	341:363	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	4	40	theme	optimum	1003:1009	arg1	conditions					1011:1020	optimum conditions	1003:1020	optimum conditions	1003:1020	Under optimum conditions, the linearity ranged from 0.04 to 300 µg L-1 with a correlation of determinations of 0.9969-0.9991.
35011480	1	41	theme	estrone	614:620	arg1	preconcentration					594:609	the preconcentration	590:609	the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples	590:704	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	42	theme	magnetic	237:244	arg1	carbon					256:261	A β-cyclodextrin-decorated magnetic activated carbon	210:261	A β-cyclodextrin-decorated magnetic activated carbon adsorbent	210:271	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	5	43	from	3.2-4.2	1264:1270	arg1	precisions					1238:1247	intraday and interday precisions	1216:1247	intraday and interday precisions at 1.1-3.4 and 3.2-4.2	1216:1270	The limits of detection and quantification were between 0.01-0.03 and 0.033-0.1 µg L-1, with intraday and interday precisions at 1.1-3.4 and 3.2-4.2.
35011480	1	44	theme	transmission	443:454	arg1	TEM					477:479	TEM	477:479	TEM	477:479	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	44	theme	transmission	443:454	arg1	techniques					329:338	various analytical techniques	310:338	various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM))	310:481	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	44	theme	transmission	443:454	arg1	microscopy					465:474	transmission electron microscopy	443:474	transmission electron microscopy (TEM)	443:480	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	4	45	theme	determinations	1090:1103	arg1	correlation					1075:1085	a correlation	1073:1085	a correlation of determinations of 0.9969-0.9991	1073:1120	Under optimum conditions, the linearity ranged from 0.04 to 300 µg L-1 with a correlation of determinations of 0.9969-0.9991.
35011480	2	46	theme	central	743:749	arg1	design					761:766	the central composite design in order to determine the experimental parameters affecting the extraction procedure	739:851	the central composite design in order to determine the experimental parameters affecting the extraction procedure	739:851	This method was optimized using the central composite design in order to determine the experimental parameters affecting the extraction procedure.
35011480	1	47	theme	activated	246:254	arg1	carbon					256:261	A β-cyclodextrin-decorated magnetic activated carbon	210:261	A β-cyclodextrin-decorated magnetic activated carbon adsorbent	210:271	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	5	48	theme	quantification	1151:1164	arg1	limits					1127:1132	The limits	1123:1132	The limits of detection and quantification	1123:1164	The limits of detection and quantification were between 0.01-0.03 and 0.033-0.1 µg L-1, with intraday and interday precisions at 1.1-3.4 and 3.2-4.2.
35011480	1	49	theme	electron	456:463	arg1	TEM					477:479	TEM	477:479	TEM	477:479	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	49	theme	electron	456:463	arg1	techniques					329:338	various analytical techniques	310:338	various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM))	310:481	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	49	theme	electron	456:463	arg1	microscopy					465:474	transmission electron microscopy	443:474	transmission electron microscopy (TEM)	443:480	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	7	50	theme	developed	1413:1421	arg1	procedure					1423:1431	The developed procedure	1409:1431	The developed procedure	1409:1431	The developed procedure demonstrated high potential as an effective technique for use in wastewater samples without significant interferences, and the adsorbent could be reused up to eight times.
35011480	1	51	dep	techniques	329:338	arg1	XRD					360:362	XRD	360:362	XRD	360:362	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	51	dep	techniques	329:338	arg1	spectroscopy					416:427	scanning electron microscopy-electron diffraction spectroscopy	366:427	scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS)	366:437	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	51	dep	techniques	329:338	arg1	techniques					329:338	various analytical techniques	310:338	various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM))	310:481	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	51	dep	techniques	329:338	arg1	microscopy					465:474	transmission electron microscopy	443:474	transmission electron microscopy (TEM)	443:480	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	51	dep	techniques	329:338	arg1	SEM-EDS					430:436	SEM-EDS	430:436	SEM-EDS	430:436	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	51	dep	techniques	329:338	arg1	TEM					477:479	TEM	477:479	TEM	477:479	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	51	dep	techniques	329:338	arg1	diffraction					347:357	X-ray diffraction	341:357	X-ray diffraction (XRD)	341:363	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	5	52	theme	intraday	1216:1223	arg1	precisions					1238:1247	intraday and interday precisions	1216:1247	intraday and interday precisions at 1.1-3.4 and 3.2-4.2	1216:1270	The limits of detection and quantification were between 0.01-0.03 and 0.033-0.1 µg L-1, with intraday and interday precisions at 1.1-3.4 and 3.2-4.2.
35011480	1	53	from	preconcentration	594:609	arg1	samples					698:704	wastewater and river water samples	671:704	samples	698:704	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	5	54	theme	detection	1137:1145	arg1	limits					1127:1132	The limits	1123:1132	The limits of detection and quantification	1123:1164	The limits of detection and quantification were between 0.01-0.03 and 0.033-0.1 µg L-1, with intraday and interday precisions at 1.1-3.4 and 3.2-4.2.
35011480	3	55	theme	liquid	921:926	arg1	chromatography					928:941	high-performance liquid chromatography	904:941	high-performance liquid chromatography equipped with a photodiode array detector (HPLC-DAD)	904:994	The quantification of hormones was achieved using high-performance liquid chromatography equipped with a photodiode array detector (HPLC-DAD).
35011480	6	56	theme	adsorption	1355:1364	arg1	capacities					1366:1375	high adsorption capacities	1350:1375	high adsorption capacities (217-294 mg g-1)	1350:1392	The equilibrium data were best described by the Langmuir isotherm model, and high adsorption capacities (217-294 mg g-1) were obtained.
35011480	6	56	theme	adsorption	1355:1364	arg1	g-1					1389:1391	217-294 mg g-1	1378:1391	217-294 mg g-1	1378:1391	The equilibrium data were best described by the Langmuir isotherm model, and high adsorption capacities (217-294 mg g-1) were obtained.
35011480	7	57	theme	high	1446:1449	arg1	potential					1451:1459	high potential	1446:1459	high potential	1446:1459	The developed procedure demonstrated high potential as an effective technique for use in wastewater samples without significant interferences, and the adsorbent could be reused up to eight times.
35011480	7	57	theme	high	1446:1449	arg1	technique					1477:1485	an effective technique	1464:1485	an effective technique for use in wastewater samples without significant interferences	1464:1549	The developed procedure demonstrated high potential as an effective technique for use in wastewater samples without significant interferences, and the adsorbent could be reused up to eight times.
35011480	1	58	used	used	506:509	arg2	adsorbent					492:500	adsorbent	492:500	adsorbent	492:500	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	3	59	theme	array	970:974	arg1	HPLC-DAD					986:993	HPLC-DAD	986:993	HPLC-DAD	986:993	The quantification of hormones was achieved using high-performance liquid chromatography equipped with a photodiode array detector (HPLC-DAD).
35011480	3	59	theme	array	970:974	arg1	detector					976:983	a photodiode array detector	957:983	a photodiode array detector (HPLC-DAD)	957:994	The quantification of hormones was achieved using high-performance liquid chromatography equipped with a photodiode array detector (HPLC-DAD).
35011480	1	60	theme	magnetic	535:542	arg1	MSPE					573:576	MSPE	573:576	MSPE	573:576	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	60	theme	magnetic	535:542	arg1	microextraction					556:570	a magnetic solid-phase microextraction	533:570	a magnetic solid-phase microextraction (MSPE) method	533:584	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	61	theme	adsorbent	263:271	arg1	carbon					256:261	A β-cyclodextrin-decorated magnetic activated carbon	210:261	A β-cyclodextrin-decorated magnetic activated carbon adsorbent	210:271	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	6	62	theme	mg	1386:1387	arg1	capacities					1366:1375	high adsorption capacities	1350:1375	high adsorption capacities (217-294 mg g-1)	1350:1392	The equilibrium data were best described by the Langmuir isotherm model, and high adsorption capacities (217-294 mg g-1) were obtained.
35011480	6	62	theme	mg	1386:1387	arg1	g-1					1389:1391	217-294 mg g-1	1378:1391	217-294 mg g-1	1378:1391	The equilibrium data were best described by the Langmuir isotherm model, and high adsorption capacities (217-294 mg g-1) were obtained.
35011480	1	63	theme	solid-phase	544:554	arg1	MSPE					573:576	MSPE	573:576	MSPE	573:576	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	63	theme	solid-phase	544:554	arg1	microextraction					556:570	a magnetic solid-phase microextraction	533:570	a magnetic solid-phase microextraction (MSPE) method	533:584	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	1	64	theme	hydrocortisone	636:649	arg1	preconcentration					594:609	the preconcentration	590:609	the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples	590:704	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	7	65	dep	eight	1592:1596	arg1	to					1589:1590	to	1589:1590	to	1589:1590	The developed procedure demonstrated high potential as an effective technique for use in wastewater samples without significant interferences, and the adsorbent could be reused up to eight times.
35011480	1	66	theme	microextraction	556:570	arg1	method					579:584	a magnetic solid-phase microextraction (MSPE) method	533:584	a magnetic solid-phase microextraction (MSPE) method	533:584	A β-cyclodextrin-decorated magnetic activated carbon adsorbent was prepared and characterized using various analytical techniques (X-ray diffraction (XRD), scanning electron microscopy-electron diffraction spectroscopy (SEM-EDS) and transmission electron microscopy (TEM)), and the adsorbent was used in the development of a magnetic solid-phase microextraction (MSPE) method for the preconcentration of estrone, β-estradiol, hydrocortisone and progesterone in wastewater and river water samples.
35011480	4	67	theme	0.9969-0.9991	1108:1120	arg1	determinations					1090:1103	determinations	1090:1103	determinations of 0.9969-0.9991	1090:1120	Under optimum conditions, the linearity ranged from 0.04 to 300 µg L-1 with a correlation of determinations of 0.9969-0.9991.
33536321	6	0	dep	center	928:933	arg1	the					914:916	the	914:916	the	914:916	PnpA ( NlpC-like protein A) has a four-domain structure with a hydrophobic and narrow access to the catalytic center and specificity for the γ-d-glutamyl-meso-diaminopimelic acid bond.
33536321	3	1	from	bonds	517:521	arg1	PG					530:531	the PG	526:531	the PG	526:531	PG biogenesis is highly dynamic and requires multiple enzymes, including several hydrolases that cleave glycosidic or amide bonds in the PG.
33536321	6	2	theme	catalytic	918:926	arg1	center					928:933	catalytic center	918:933	catalytic center	918:933	PnpA ( NlpC-like protein A) has a four-domain structure with a hydrophobic and narrow access to the catalytic center and specificity for the γ-d-glutamyl-meso-diaminopimelic acid bond.
33536321	10	3	theme	specific	2090:2097	arg1	enzymes					2099:2105	specific enzymes	2090:2105	specific enzymes that cleave or join its components	2090:2140	Although PG provides structural integrity and support for anchoring other components of the cell envelope, it is constantly being remodeled through the action of specific enzymes that cleave or join its components.
33536321	4	4	theme	NlpC/P60-containing	607:625	arg1	peptidase					627:635	an NlpC/P60-containing peptidase	604:635	an NlpC/P60-containing peptidase from Photobacterium damselae subsp	604:670	This work describes the structural and functional characterization of an NlpC/P60-containing peptidase from Photobacterium damselae subsp.
33536321	13	5	theme	bacteria	2511:2518	arg1	PG					2500:2501	the PG	2496:2501	the PG of some bacteria and its inability to cleave others	2496:2553	The specificity of PnpA for the PG of some bacteria and its inability to cleave others may be explained by differences in the structure of the PG mesh and not by different muropeptide composition.
33536321	6	6	theme	protein	835:841	arg1	PnpA					818:821	PnpA	818:821	PnpA ( NlpC-like protein A)	818:844	PnpA ( NlpC-like protein A) has a four-domain structure with a hydrophobic and narrow access to the catalytic center and specificity for the γ-d-glutamyl-meso-diaminopimelic acid bond.
33536321	6	6	theme	protein	835:841	arg1	A					843:843	NlpC-like protein A	825:843	NlpC-like protein A	825:843	PnpA ( NlpC-like protein A) has a four-domain structure with a hydrophobic and narrow access to the catalytic center and specificity for the γ-d-glutamyl-meso-diaminopimelic acid bond.
33536321	9	7	theme	muropeptide	1432:1442	arg1	composition					1444:1454	the muropeptide composition	1428:1454	the muropeptide composition of PG susceptible and resistant	1428:1486	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	1	8	theme	Bacteria	131:138	arg1	Peptidoglycan					92:104	the Peptidoglycan	88:104	the Peptidoglycan of Potentially Competing Bacteria	88:138	piscicida Cleaves the Peptidoglycan of Potentially Competing Bacteria.
33536321	13	9	theme	PnpA	2487:2490	arg1	specificity					2472:2482	The specificity	2468:2482	The specificity of PnpA for the PG of some bacteria and its inability to cleave others	2468:2553	The specificity of PnpA for the PG of some bacteria and its inability to cleave others may be explained by differences in the structure of the PG mesh and not by different muropeptide composition.
33536321	13	10	theme	muropeptide	2640:2650	arg1	composition					2652:2662	different muropeptide composition	2630:2662	different muropeptide composition	2630:2662	The specificity of PnpA for the PG of some bacteria and its inability to cleave others may be explained by differences in the structure of the PG mesh and not by different muropeptide composition.
33536321	6	11	theme	narrow	897:902	arg1	access					904:909	a hydrophobic and narrow access	879:909	a hydrophobic and narrow access to the catalytic center and specificity for the γ-d-glutamyl-meso-diaminopimelic acid bond	879:1000	PnpA ( NlpC-like protein A) has a four-domain structure with a hydrophobic and narrow access to the catalytic center and specificity for the γ-d-glutamyl-meso-diaminopimelic acid bond.
33536321	9	12	theme	PG.IMPORTANCE	1699:1711	arg1	PG					1728:1729	PG	1728:1729	PG	1728:1729	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	9	12	theme	PG.IMPORTANCE	1699:1711	arg1	Peptidoglycan					1713:1725	the PG.IMPORTANCE Peptidoglycan	1695:1725	the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell	1695:1925	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	9	13	theme	major	1737:1741	arg1	component					1743:1751	a major component	1735:1751	a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides	1735:1859	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	13	14	theme	PG	2611:2612	arg1	mesh					2614:2617	the PG mesh	2607:2617	the PG mesh	2607:2617	The specificity of PnpA for the PG of some bacteria and its inability to cleave others may be explained by differences in the structure of the PG mesh and not by different muropeptide composition.
33536321	9	15	theme	susceptible	1462:1472	arg1	PG					1459:1460	PG	1459:1460	PG susceptible and resistant	1459:1486	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	8	16	theme	Vibrio	1206:1211	arg1	anguillarum					1213:1223	Vibrio anguillarum	1206:1223	Vibrio anguillarum	1206:1223	Interestingly, it is secreted by the Phdp type II secretion system and degrades the PG of Vibrio anguillarum and Vibrio vulnificus This suggests that PnpA is used by Phdp to gain an advantage over bacteria that compete for the same resources or to obtain nutrients in nutrient-scarce environments.
33536321	9	17	from	Peptidoglycan	1713:1725	arg1	organization					1659:1670	the three-dimensional organization	1637:1670	the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell	1637:1925	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	7	18	theme	bacterial	1097:1105	arg1	species					1107:1113	several Gram-negative and Gram-positive bacterial species	1057:1113	several Gram-negative and Gram-positive bacterial species	1057:1113	However, PnpA does not cleave the PG of Phdp or PG of several Gram-negative and Gram-positive bacterial species.
33536321	8	19	used	used	1274:1277	arg2	PnpA					1266:1269	PnpA	1266:1269	PnpA	1266:1269	Interestingly, it is secreted by the Phdp type II secretion system and degrades the PG of Vibrio anguillarum and Vibrio vulnificus This suggests that PnpA is used by Phdp to gain an advantage over bacteria that compete for the same resources or to obtain nutrients in nutrient-scarce environments.
33536321	9	20	theme	resistant	1478:1486	arg1	PG					1459:1460	PG	1459:1460	PG susceptible and resistant	1459:1486	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	3	21	theme	multiple	438:445	arg1	enzymes					447:453	multiple enzymes	438:453	multiple enzymes	438:453	PG biogenesis is highly dynamic and requires multiple enzymes, including several hydrolases that cleave glycosidic or amide bonds in the PG.
33536321	3	21	theme	multiple	438:445	arg1	hydrolases					474:483	several hydrolases	466:483	several hydrolases that cleave glycosidic or amide bonds in the PG	466:531	PG biogenesis is highly dynamic and requires multiple enzymes, including several hydrolases that cleave glycosidic or amide bonds in the PG.
33536321	5	22	theme	warm-water	747:756	arg1	fish					765:768	warm-water marine fish	747:768	warm-water marine fish	747:768	piscicida (Phdp), a Gram-negative bacterium that causes high mortality of warm-water marine fish with great impact for the aquaculture industry.
33536321	12	23	dep	able	2344:2347	arg1	enzyme					2292:2297	an enzyme	2289:2297	an enzyme that is secreted into the environment and is able to cleave the PG of potentially competing bacteria, either to gain a competitive advantage and/or to obtain nutrients	2289:2465	piscicida, a bacterium that causes high mortality in warm-water marine fish, produces PnpA, an enzyme that is secreted into the environment and is able to cleave the PG of potentially competing bacteria, either to gain a competitive advantage and/or to obtain nutrients.
33536321	12	23	dep	able	2344:2347	arg1	obtain					2450:2455	obtain	2450:2455	to obtain nutrients	2447:2465	piscicida, a bacterium that causes high mortality in warm-water marine fish, produces PnpA, an enzyme that is secreted into the environment and is able to cleave the PG of potentially competing bacteria, either to gain a competitive advantage and/or to obtain nutrients.
33536321	12	23	dep	able	2344:2347	arg1	gain					2411:2414	gain	2411:2414	to gain a competitive advantage	2408:2438	piscicida, a bacterium that causes high mortality in warm-water marine fish, produces PnpA, an enzyme that is secreted into the environment and is able to cleave the PG of potentially competing bacteria, either to gain a competitive advantage and/or to obtain nutrients.
33536321	12	23	dep	able	2344:2347	arg1	able					2344:2347	able	2344:2347	able	2344:2347	piscicida, a bacterium that causes high mortality in warm-water marine fish, produces PnpA, an enzyme that is secreted into the environment and is able to cleave the PG of potentially competing bacteria, either to gain a competitive advantage and/or to obtain nutrients.
33536321	12	23	dep	able	2344:2347	arg1	PnpA					2283:2286	PnpA	2283:2286	PnpA	2283:2286	piscicida, a bacterium that causes high mortality in warm-water marine fish, produces PnpA, an enzyme that is secreted into the environment and is able to cleave the PG of potentially competing bacteria, either to gain a competitive advantage and/or to obtain nutrients.
33536321	12	23	dep	able	2344:2347	arg1	cleave					2352:2357	cleave	2352:2357	to cleave the PG of potentially competing bacteria	2349:2398	piscicida, a bacterium that causes high mortality in warm-water marine fish, produces PnpA, an enzyme that is secreted into the environment and is able to cleave the PG of potentially competing bacteria, either to gain a competitive advantage and/or to obtain nutrients.
33536321	2	24	theme	mesh-like	219:227	arg1	structure					229:237	a mesh-like structure	217:237	a mesh-like structure enwrapping the bacteria that is essential for maintaining structural integrity and providing support for anchoring other components of the cell envelope	217:390	Peptidoglycan (PG) is a major component of the bacterial cell wall, forming a mesh-like structure enwrapping the bacteria that is essential for maintaining structural integrity and providing support for anchoring other components of the cell envelope.
33536321	8	25	theme	vulnificus	1236:1245	arg1	PG					1200:1201	the PG	1196:1201	the PG of Vibrio anguillarum and Vibrio vulnificus This suggests that PnpA is used by Phdp to gain an advantage over bacteria that compete for the same resources or to obtain nutrients in nutrient-scarce environments	1196:1411	Interestingly, it is secreted by the Phdp type II secretion system and degrades the PG of Vibrio anguillarum and Vibrio vulnificus This suggests that PnpA is used by Phdp to gain an advantage over bacteria that compete for the same resources or to obtain nutrients in nutrient-scarce environments.
33536321	6	26	theme	γ-d-glutamyl-meso-diaminopimelic	959:990	arg1	bond					997:1000	the γ-d-glutamyl-meso-diaminopimelic acid bond	955:1000	the γ-d-glutamyl-meso-diaminopimelic acid bond	955:1000	PnpA ( NlpC-like protein A) has a four-domain structure with a hydrophobic and narrow access to the catalytic center and specificity for the γ-d-glutamyl-meso-diaminopimelic acid bond.
33536321	7	27	theme	several	1057:1063	arg1	species					1107:1113	several Gram-negative and Gram-positive bacterial species	1057:1113	several Gram-negative and Gram-positive bacterial species	1057:1113	However, PnpA does not cleave the PG of Phdp or PG of several Gram-negative and Gram-positive bacterial species.
33536321	7	28	theme	PG	1051:1052	arg1	PG					1037:1038	the PG	1033:1038	the PG of Phdp or PG of several Gram-negative and Gram-positive bacterial species	1033:1113	However, PnpA does not cleave the PG of Phdp or PG of several Gram-negative and Gram-positive bacterial species.
33536321	9	29	theme	PnpA	1517:1520	arg1	activity					1505:1512	the catalytic activity	1491:1512	the catalytic activity of PnpA	1491:1520	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	6	30	contain	has	846:848	arg1	PnpA					818:821	PnpA	818:821	PnpA ( NlpC-like protein A)	818:844	PnpA ( NlpC-like protein A) has a four-domain structure with a hydrophobic and narrow access to the catalytic center and specificity for the γ-d-glutamyl-meso-diaminopimelic acid bond.
33536321	6	30	contain	has	846:848	arg1	A					843:843	NlpC-like protein A	825:843	NlpC-like protein A	825:843	PnpA ( NlpC-like protein A) has a four-domain structure with a hydrophobic and narrow access to the catalytic center and specificity for the γ-d-glutamyl-meso-diaminopimelic acid bond.
33536321	6	30	contain	has	846:848	arg2	structure					864:872	a four-domain structure	850:872	a four-domain structure with a hydrophobic and narrow access to the catalytic center and specificity for the γ-d-glutamyl-meso-diaminopimelic acid bond	850:1000	PnpA ( NlpC-like protein A) has a four-domain structure with a hydrophobic and narrow access to the catalytic center and specificity for the γ-d-glutamyl-meso-diaminopimelic acid bond.
33536321	10	31	theme	envelope	2025:2032	arg1	components					2002:2011	other components	1996:2011	other components of the cell envelope	1996:2032	Although PG provides structural integrity and support for anchoring other components of the cell envelope, it is constantly being remodeled through the action of specific enzymes that cleave or join its components.
33536321	9	32	theme	cell	1770:1773	arg1	wall					1775:1778	the bacterial cell wall	1756:1778	the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides	1756:1859	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	12	33	theme	competitive	2418:2428	arg1	advantage					2430:2438	a competitive advantage	2416:2438	a competitive advantage	2416:2438	piscicida, a bacterium that causes high mortality in warm-water marine fish, produces PnpA, an enzyme that is secreted into the environment and is able to cleave the PG of potentially competing bacteria, either to gain a competitive advantage and/or to obtain nutrients.
33536321	5	34	theme	aquaculture	796:806	arg1	industry					808:815	the aquaculture industry	792:815	the aquaculture industry	792:815	piscicida (Phdp), a Gram-negative bacterium that causes high mortality of warm-water marine fish with great impact for the aquaculture industry.
33536321	9	35	theme	global	1538:1543	arg1	content					1545:1551	the global content	1534:1551	the global content of muropeptides	1534:1567	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	9	35	theme	global	1538:1543	arg1	similar					1572:1578	similar	1572:1578	similar	1572:1578	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	12	36	theme	high	2232:2235	arg1	mortality					2237:2245	high mortality	2232:2245	high mortality in warm-water marine fish	2232:2271	piscicida, a bacterium that causes high mortality in warm-water marine fish, produces PnpA, an enzyme that is secreted into the environment and is able to cleave the PG of potentially competing bacteria, either to gain a competitive advantage and/or to obtain nutrients.
33536321	5	37	theme	fish	765:768	arg1	mortality					734:742	high mortality	729:742	high mortality of warm-water marine fish with great impact for the aquaculture industry	729:815	piscicida (Phdp), a Gram-negative bacterium that causes high mortality of warm-water marine fish with great impact for the aquaculture industry.
33536321	9	38	theme	long	1790:1793	arg1	chains					1795:1800	long chains	1790:1800	long chains of two alternating sugars interconnected by short peptides	1790:1859	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	12	39	theme	warm-water	2250:2259	arg1	fish					2268:2271	warm-water marine fish	2250:2271	warm-water marine fish	2250:2271	piscicida, a bacterium that causes high mortality in warm-water marine fish, produces PnpA, an enzyme that is secreted into the environment and is able to cleave the PG of potentially competing bacteria, either to gain a competitive advantage and/or to obtain nutrients.
33536321	5	40	theme	great	775:779	arg1	impact					781:786	great impact	775:786	great impact for the aquaculture industry	775:815	piscicida (Phdp), a Gram-negative bacterium that causes high mortality of warm-water marine fish with great impact for the aquaculture industry.
33536321	3	41	theme	PG	393:394	arg1	biogenesis					396:405	PG biogenesis	393:405	PG biogenesis	393:405	PG biogenesis is highly dynamic and requires multiple enzymes, including several hydrolases that cleave glycosidic or amide bonds in the PG.
33536321	10	42	theme	structural	1949:1958	arg1	integrity					1960:1968	structural integrity	1949:1968	structural integrity	1949:1968	Although PG provides structural integrity and support for anchoring other components of the cell envelope, it is constantly being remodeled through the action of specific enzymes that cleave or join its components.
33536321	9	43	theme	alternating	1809:1819	arg1	sugars					1821:1826	two alternating sugars	1805:1826	two alternating sugars interconnected by short peptides	1805:1859	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	0	44	theme	NlpC/P60	11:18	arg1	Endopeptidase					20:32	A Secreted NlpC/P60 Endopeptidase	0:32	A Secreted NlpC/P60 Endopeptidase from Photobacterium damselae subsp.	0:68	A Secreted NlpC/P60 Endopeptidase from Photobacterium damselae subsp.
33536321	13	45	from	differences	2575:2585	arg1	structure					2594:2602	the structure	2590:2602	the structure of the PG mesh	2590:2617	The specificity of PnpA for the PG of some bacteria and its inability to cleave others may be explained by differences in the structure of the PG mesh and not by different muropeptide composition.
33536321	1	46	theme	Competing	121:129	arg1	Bacteria					131:138	Potentially Competing Bacteria	109:138	Potentially Competing Bacteria	109:138	piscicida Cleaves the Peptidoglycan of Potentially Competing Bacteria.
33536321	9	47	theme	interconnected	1828:1841	arg1	sugars					1821:1826	two alternating sugars	1805:1826	two alternating sugars interconnected by short peptides	1805:1859	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	13	48	theme	different	2630:2638	arg1	composition					2652:2662	different muropeptide composition	2630:2662	different muropeptide composition	2630:2662	The specificity of PnpA for the PG of some bacteria and its inability to cleave others may be explained by differences in the structure of the PG mesh and not by different muropeptide composition.
33536321	8	49	theme	nutrient-scarce	1384:1398	arg1	environments					1400:1411	nutrient-scarce environments	1384:1411	nutrient-scarce environments	1384:1411	Interestingly, it is secreted by the Phdp type II secretion system and degrades the PG of Vibrio anguillarum and Vibrio vulnificus This suggests that PnpA is used by Phdp to gain an advantage over bacteria that compete for the same resources or to obtain nutrients in nutrient-scarce environments.
33536321	9	50	dep	Peptidoglycan	1713:1725	arg1	component					1743:1751	a major component	1735:1751	a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides	1735:1859	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	5	51	with	mortality	734:742	arg1	impact					781:786	great impact	775:786	great impact for the aquaculture industry	775:815	piscicida (Phdp), a Gram-negative bacterium that causes high mortality of warm-water marine fish with great impact for the aquaculture industry.
33536321	9	52	theme	short	1846:1850	arg1	peptides					1852:1859	short peptides	1846:1859	short peptides	1846:1859	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	12	53	theme	competing	2381:2389	arg1	bacteria					2391:2398	potentially competing bacteria	2369:2398	potentially competing bacteria	2369:2398	piscicida, a bacterium that causes high mortality in warm-water marine fish, produces PnpA, an enzyme that is secreted into the environment and is able to cleave the PG of potentially competing bacteria, either to gain a competitive advantage and/or to obtain nutrients.
33536321	2	54	theme	major	165:169	arg1	Peptidoglycan					141:153	Peptidoglycan	141:153	Peptidoglycan (PG)	141:158	Peptidoglycan (PG) is a major component of the bacterial cell wall, forming a mesh-like structure enwrapping the bacteria that is essential for maintaining structural integrity and providing support for anchoring other components of the cell envelope.
33536321	2	54	theme	major	165:169	arg1	component					171:179	a major component	163:179	a major component	163:179	Peptidoglycan (PG) is a major component of the bacterial cell wall, forming a mesh-like structure enwrapping the bacteria that is essential for maintaining structural integrity and providing support for anchoring other components of the cell envelope.
33536321	2	55	theme	other	354:358	arg1	components					360:369	other components	354:369	other components of the cell envelope	354:390	Peptidoglycan (PG) is a major component of the bacterial cell wall, forming a mesh-like structure enwrapping the bacteria that is essential for maintaining structural integrity and providing support for anchoring other components of the cell envelope.
33536321	0	56	theme	damselae	54:61	arg1	subsp					63:67	Photobacterium damselae subsp	39:67	Photobacterium damselae subsp	39:67	A Secreted NlpC/P60 Endopeptidase from Photobacterium damselae subsp.
33536321	8	57	dep	PG	1200:1201	arg1	suggests					1252:1259	suggests	1252:1259	suggests that PnpA is used by Phdp to gain an advantage over bacteria that compete for the same resources or to obtain nutrients in nutrient-scarce environments	1252:1411	Interestingly, it is secreted by the Phdp type II secretion system and degrades the PG of Vibrio anguillarum and Vibrio vulnificus This suggests that PnpA is used by Phdp to gain an advantage over bacteria that compete for the same resources or to obtain nutrients in nutrient-scarce environments.
33536321	2	58	theme	wall	203:206	arg1	Peptidoglycan					141:153	Peptidoglycan	141:153	Peptidoglycan (PG)	141:158	Peptidoglycan (PG) is a major component of the bacterial cell wall, forming a mesh-like structure enwrapping the bacteria that is essential for maintaining structural integrity and providing support for anchoring other components of the cell envelope.
33536321	2	58	theme	wall	203:206	arg1	component					171:179	a major component	163:179	a major component	163:179	Peptidoglycan (PG) is a major component of the bacterial cell wall, forming a mesh-like structure enwrapping the bacteria that is essential for maintaining structural integrity and providing support for anchoring other components of the cell envelope.
33536321	4	59	theme	peptidase	627:635	arg1	characterization					584:599	the structural and functional characterization	554:599	the structural and functional characterization of an NlpC/P60-containing peptidase from Photobacterium damselae subsp	554:670	This work describes the structural and functional characterization of an NlpC/P60-containing peptidase from Photobacterium damselae subsp.
33536321	3	60	theme	several	466:472	arg1	hydrolases					474:483	several hydrolases	466:483	several hydrolases that cleave glycosidic or amide bonds in the PG	466:531	PG biogenesis is highly dynamic and requires multiple enzymes, including several hydrolases that cleave glycosidic or amide bonds in the PG.
33536321	2	61	theme	cell	378:381	arg1	envelope					383:390	the cell envelope	374:390	the cell envelope	374:390	Peptidoglycan (PG) is a major component of the bacterial cell wall, forming a mesh-like structure enwrapping the bacteria that is essential for maintaining structural integrity and providing support for anchoring other components of the cell envelope.
33536321	11	62	theme	damselae	2181:2188	arg1	subsp					2190:2194	Photobacterium damselae subsp	2166:2194	Photobacterium damselae subsp	2166:2194	Here, it is shown that Photobacterium damselae subsp.
33536321	9	63	theme	three-dimensional	1641:1657	arg1	organization					1659:1670	the three-dimensional organization	1637:1670	the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell	1637:1925	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	4	64	theme	Photobacterium	642:655	arg1	subsp					666:670	Photobacterium damselae subsp	642:670	Photobacterium damselae subsp	642:670	This work describes the structural and functional characterization of an NlpC/P60-containing peptidase from Photobacterium damselae subsp.
33536321	8	65	theme	type	1158:1161	arg1	system					1176:1181	the Phdp type II secretion system	1149:1181	the Phdp type II secretion system	1149:1181	Interestingly, it is secreted by the Phdp type II secretion system and degrades the PG of Vibrio anguillarum and Vibrio vulnificus This suggests that PnpA is used by Phdp to gain an advantage over bacteria that compete for the same resources or to obtain nutrients in nutrient-scarce environments.
33536321	10	66	theme	enzymes	2099:2105	arg1	action					2080:2085	the action	2076:2085	the action of specific enzymes that cleave or join its components	2076:2140	Although PG provides structural integrity and support for anchoring other components of the cell envelope, it is constantly being remodeled through the action of specific enzymes that cleave or join its components.
33536321	8	67	theme	secretion	1166:1174	arg1	system					1176:1181	the Phdp type II secretion system	1149:1181	the Phdp type II secretion system	1149:1181	Interestingly, it is secreted by the Phdp type II secretion system and degrades the PG of Vibrio anguillarum and Vibrio vulnificus This suggests that PnpA is used by Phdp to gain an advantage over bacteria that compete for the same resources or to obtain nutrients in nutrient-scarce environments.
33536321	9	68	theme	muropeptides	1679:1690	arg1	organization					1659:1670	the three-dimensional organization	1637:1670	the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell	1637:1925	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	9	69	theme	bacterial	1912:1920	arg1	cell					1922:1925	the bacterial cell	1908:1925	the bacterial cell	1908:1925	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	3	70	theme	or	508:509	arg1	bonds					517:521	glycosidic or amide bonds	497:521	glycosidic or amide bonds in the PG	497:531	PG biogenesis is highly dynamic and requires multiple enzymes, including several hydrolases that cleave glycosidic or amide bonds in the PG.
33536321	6	71	theme	NlpC-like	825:833	arg1	PnpA					818:821	PnpA	818:821	PnpA ( NlpC-like protein A)	818:844	PnpA ( NlpC-like protein A) has a four-domain structure with a hydrophobic and narrow access to the catalytic center and specificity for the γ-d-glutamyl-meso-diaminopimelic acid bond.
33536321	6	71	theme	NlpC-like	825:833	arg1	A					843:843	NlpC-like protein A	825:843	NlpC-like protein A	825:843	PnpA ( NlpC-like protein A) has a four-domain structure with a hydrophobic and narrow access to the catalytic center and specificity for the γ-d-glutamyl-meso-diaminopimelic acid bond.
33536321	12	72	from	mortality	2237:2245	arg1	fish					2268:2271	warm-water marine fish	2250:2271	warm-water marine fish	2250:2271	piscicida, a bacterium that causes high mortality in warm-water marine fish, produces PnpA, an enzyme that is secreted into the environment and is able to cleave the PG of potentially competing bacteria, either to gain a competitive advantage and/or to obtain nutrients.
33536321	9	73	theme	composition	1444:1454	arg1	Comparison					1414:1423	Comparison	1414:1423	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA	1414:1520	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	7	74	theme	species	1107:1113	arg1	PG					1051:1052	PG	1051:1052	PG	1051:1052	However, PnpA does not cleave the PG of Phdp or PG of several Gram-negative and Gram-positive bacterial species.
33536321	7	74	theme	species	1107:1113	arg1	Phdp					1043:1046	Phdp	1043:1046	Phdp	1043:1046	However, PnpA does not cleave the PG of Phdp or PG of several Gram-negative and Gram-positive bacterial species.
33536321	9	75	theme	PG	1459:1460	arg1	composition					1444:1454	the muropeptide composition	1428:1454	the muropeptide composition of PG susceptible and resistant	1428:1486	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	5	76	theme	high	729:732	arg1	mortality					734:742	high mortality	729:742	high mortality of warm-water marine fish with great impact for the aquaculture industry	729:815	piscicida (Phdp), a Gram-negative bacterium that causes high mortality of warm-water marine fish with great impact for the aquaculture industry.
33536321	13	77	theme	mesh	2614:2617	arg1	structure					2594:2602	the structure	2590:2602	the structure of the PG mesh	2590:2617	The specificity of PnpA for the PG of some bacteria and its inability to cleave others may be explained by differences in the structure of the PG mesh and not by different muropeptide composition.
33536321	7	78	theme	Gram-negative	1065:1077	arg1	species					1107:1113	several Gram-negative and Gram-positive bacterial species	1057:1113	several Gram-negative and Gram-positive bacterial species	1057:1113	However, PnpA does not cleave the PG of Phdp or PG of several Gram-negative and Gram-positive bacterial species.
33536321	4	79	from	subsp	666:670	arg1	characterization					584:599	the structural and functional characterization	554:599	the structural and functional characterization of an NlpC/P60-containing peptidase from Photobacterium damselae subsp	554:670	This work describes the structural and functional characterization of an NlpC/P60-containing peptidase from Photobacterium damselae subsp.
33536321	4	79	from	subsp	666:670	arg1	peptidase					627:635	an NlpC/P60-containing peptidase	604:635	an NlpC/P60-containing peptidase from Photobacterium damselae subsp	604:670	This work describes the structural and functional characterization of an NlpC/P60-containing peptidase from Photobacterium damselae subsp.
33536321	8	80	theme	anguillarum	1213:1223	arg1	PG					1200:1201	the PG	1196:1201	the PG of Vibrio anguillarum and Vibrio vulnificus This suggests that PnpA is used by Phdp to gain an advantage over bacteria that compete for the same resources or to obtain nutrients in nutrient-scarce environments	1196:1411	Interestingly, it is secreted by the Phdp type II secretion system and degrades the PG of Vibrio anguillarum and Vibrio vulnificus This suggests that PnpA is used by Phdp to gain an advantage over bacteria that compete for the same resources or to obtain nutrients in nutrient-scarce environments.
33536321	6	81	theme	acid	992:995	arg1	bond					997:1000	the γ-d-glutamyl-meso-diaminopimelic acid bond	955:1000	the γ-d-glutamyl-meso-diaminopimelic acid bond	955:1000	PnpA ( NlpC-like protein A) has a four-domain structure with a hydrophobic and narrow access to the catalytic center and specificity for the γ-d-glutamyl-meso-diaminopimelic acid bond.
33536321	5	82	theme	Gram-negative	693:705	arg1	piscicida					673:681	piscicida	673:681	piscicida (Phdp)	673:688	piscicida (Phdp), a Gram-negative bacterium that causes high mortality of warm-water marine fish with great impact for the aquaculture industry.
33536321	5	82	theme	Gram-negative	693:705	arg1	bacterium					707:715	a Gram-negative bacterium	691:715	a Gram-negative bacterium that causes high mortality of warm-water marine fish with great impact for the aquaculture industry	691:815	piscicida (Phdp), a Gram-negative bacterium that causes high mortality of warm-water marine fish with great impact for the aquaculture industry.
33536321	7	83	theme	Phdp	1043:1046	arg1	PG					1037:1038	the PG	1033:1038	the PG of Phdp or PG of several Gram-negative and Gram-positive bacterial species	1033:1113	However, PnpA does not cleave the PG of Phdp or PG of several Gram-negative and Gram-positive bacterial species.
33536321	9	84	theme	catalytic	1495:1503	arg1	activity					1505:1512	the catalytic activity	1491:1512	the catalytic activity of PnpA	1491:1520	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	8	85	theme	Vibrio	1229:1234	arg1	vulnificus					1236:1245	Vibrio vulnificus	1229:1245	Vibrio vulnificus	1229:1245	Interestingly, it is secreted by the Phdp type II secretion system and degrades the PG of Vibrio anguillarum and Vibrio vulnificus This suggests that PnpA is used by Phdp to gain an advantage over bacteria that compete for the same resources or to obtain nutrients in nutrient-scarce environments.
33536321	9	86	from	organization	1659:1670	arg1	PG					1728:1729	PG	1728:1729	PG	1728:1729	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	9	86	from	organization	1659:1670	arg1	Peptidoglycan					1713:1725	the PG.IMPORTANCE Peptidoglycan	1695:1725	the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell	1695:1925	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	7	87	theme	Gram-positive	1083:1095	arg1	species					1107:1113	several Gram-negative and Gram-positive bacterial species	1057:1113	several Gram-negative and Gram-positive bacterial species	1057:1113	However, PnpA does not cleave the PG of Phdp or PG of several Gram-negative and Gram-positive bacterial species.
33536321	10	88	theme	other	1996:2000	arg1	components					2002:2011	other components	1996:2011	other components of the cell envelope	1996:2032	Although PG provides structural integrity and support for anchoring other components of the cell envelope, it is constantly being remodeled through the action of specific enzymes that cleave or join its components.
33536321	9	89	theme	bacterial	1760:1768	arg1	wall					1775:1778	the bacterial cell wall	1756:1778	the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides	1756:1859	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	10	90	theme	cell	2020:2023	arg1	envelope					2025:2032	the cell envelope	2016:2032	the cell envelope	2016:2032	Although PG provides structural integrity and support for anchoring other components of the cell envelope, it is constantly being remodeled through the action of specific enzymes that cleave or join its components.
33536321	12	91	theme	marine	2261:2266	arg1	fish					2268:2271	warm-water marine fish	2250:2271	warm-water marine fish	2250:2271	piscicida, a bacterium that causes high mortality in warm-water marine fish, produces PnpA, an enzyme that is secreted into the environment and is able to cleave the PG of potentially competing bacteria, either to gain a competitive advantage and/or to obtain nutrients.
33536321	9	92	theme	wall	1775:1778	arg1	component					1743:1751	a major component	1735:1751	a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides	1735:1859	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	5	93	theme	marine	758:763	arg1	fish					765:768	warm-water marine fish	747:768	warm-water marine fish	747:768	piscicida (Phdp), a Gram-negative bacterium that causes high mortality of warm-water marine fish with great impact for the aquaculture industry.
33536321	2	94	theme	structural	297:306	arg1	integrity					308:316	structural integrity	297:316	structural integrity	297:316	Peptidoglycan (PG) is a major component of the bacterial cell wall, forming a mesh-like structure enwrapping the bacteria that is essential for maintaining structural integrity and providing support for anchoring other components of the cell envelope.
33536321	0	95	from	subsp	63:67	arg1	Endopeptidase					20:32	A Secreted NlpC/P60 Endopeptidase	0:32	A Secreted NlpC/P60 Endopeptidase from Photobacterium damselae subsp.	0:68	A Secreted NlpC/P60 Endopeptidase from Photobacterium damselae subsp.
33536321	9	96	theme	muropeptides	1556:1567	arg1	content					1545:1551	the global content	1534:1551	the global content of muropeptides	1534:1567	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	9	96	theme	muropeptides	1556:1567	arg1	similar					1572:1578	similar	1572:1578	similar	1572:1578	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	8	97	theme	same	1343:1346	arg1	resources					1348:1356	the same resources	1339:1356	the same resources	1339:1356	Interestingly, it is secreted by the Phdp type II secretion system and degrades the PG of Vibrio anguillarum and Vibrio vulnificus This suggests that PnpA is used by Phdp to gain an advantage over bacteria that compete for the same resources or to obtain nutrients in nutrient-scarce environments.
33536321	0	98	theme	Secreted	2:9	arg1	Endopeptidase					20:32	A Secreted NlpC/P60 Endopeptidase	0:32	A Secreted NlpC/P60 Endopeptidase from Photobacterium damselae subsp.	0:68	A Secreted NlpC/P60 Endopeptidase from Photobacterium damselae subsp.
33536321	11	99	theme	Photobacterium	2166:2179	arg1	subsp					2190:2194	Photobacterium damselae subsp	2166:2194	Photobacterium damselae subsp	2166:2194	Here, it is shown that Photobacterium damselae subsp.
33536321	4	100	theme	structural	558:567	arg1	characterization					584:599	the structural and functional characterization	554:599	the structural and functional characterization of an NlpC/P60-containing peptidase from Photobacterium damselae subsp	554:670	This work describes the structural and functional characterization of an NlpC/P60-containing peptidase from Photobacterium damselae subsp.
33536321	2	101	theme	bacterial	188:196	arg1	wall					203:206	the bacterial cell wall	184:206	the bacterial cell wall	184:206	Peptidoglycan (PG) is a major component of the bacterial cell wall, forming a mesh-like structure enwrapping the bacteria that is essential for maintaining structural integrity and providing support for anchoring other components of the cell envelope.
33536321	2	102	theme	envelope	383:390	arg1	components					360:369	other components	354:369	other components of the cell envelope	354:390	Peptidoglycan (PG) is a major component of the bacterial cell wall, forming a mesh-like structure enwrapping the bacteria that is essential for maintaining structural integrity and providing support for anchoring other components of the cell envelope.
33536321	9	103	theme	sugars	1821:1826	arg1	chains					1795:1800	long chains	1790:1800	long chains of two alternating sugars interconnected by short peptides	1790:1859	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	4	104	theme	functional	573:582	arg1	characterization					584:599	the structural and functional characterization	554:599	the structural and functional characterization of an NlpC/P60-containing peptidase from Photobacterium damselae subsp	554:670	This work describes the structural and functional characterization of an NlpC/P60-containing peptidase from Photobacterium damselae subsp.
33536321	0	105	theme	Photobacterium	39:52	arg1	subsp					63:67	Photobacterium damselae subsp	39:67	Photobacterium damselae subsp	39:67	A Secreted NlpC/P60 Endopeptidase from Photobacterium damselae subsp.
33536321	6	106	theme	hydrophobic	881:891	arg1	access					904:909	a hydrophobic and narrow access	879:909	a hydrophobic and narrow access to the catalytic center and specificity for the γ-d-glutamyl-meso-diaminopimelic acid bond	879:1000	PnpA ( NlpC-like protein A) has a four-domain structure with a hydrophobic and narrow access to the catalytic center and specificity for the γ-d-glutamyl-meso-diaminopimelic acid bond.
33536321	6	107	with	structure	864:872	arg1	access					904:909	a hydrophobic and narrow access	879:909	a hydrophobic and narrow access to the catalytic center and specificity for the γ-d-glutamyl-meso-diaminopimelic acid bond	879:1000	PnpA ( NlpC-like protein A) has a four-domain structure with a hydrophobic and narrow access to the catalytic center and specificity for the γ-d-glutamyl-meso-diaminopimelic acid bond.
33536321	12	108	theme	bacteria	2391:2398	arg1	PG					2363:2364	the PG	2359:2364	the PG of potentially competing bacteria	2359:2398	piscicida, a bacterium that causes high mortality in warm-water marine fish, produces PnpA, an enzyme that is secreted into the environment and is able to cleave the PG of potentially competing bacteria, either to gain a competitive advantage and/or to obtain nutrients.
33536321	4	109	from	characterization	584:599	arg1	subsp					666:670	Photobacterium damselae subsp	642:670	Photobacterium damselae subsp	642:670	This work describes the structural and functional characterization of an NlpC/P60-containing peptidase from Photobacterium damselae subsp.
33536321	13	110	theme	inability	2528:2536	arg1	PG					2500:2501	the PG	2496:2501	the PG of some bacteria and its inability to cleave others	2496:2553	The specificity of PnpA for the PG of some bacteria and its inability to cleave others may be explained by differences in the structure of the PG mesh and not by different muropeptide composition.
33536321	3	111	theme	amide	511:515	arg1	bonds					517:521	glycosidic or amide bonds	497:521	glycosidic or amide bonds in the PG	497:531	PG biogenesis is highly dynamic and requires multiple enzymes, including several hydrolases that cleave glycosidic or amide bonds in the PG.
33536321	6	112	theme	four-domain	852:862	arg1	structure					864:872	a four-domain structure	850:872	a four-domain structure with a hydrophobic and narrow access to the catalytic center and specificity for the γ-d-glutamyl-meso-diaminopimelic acid bond	850:1000	PnpA ( NlpC-like protein A) has a four-domain structure with a hydrophobic and narrow access to the catalytic center and specificity for the γ-d-glutamyl-meso-diaminopimelic acid bond.
33536321	2	113	theme	cell	198:201	arg1	wall					203:206	the bacterial cell wall	184:206	the bacterial cell wall	184:206	Peptidoglycan (PG) is a major component of the bacterial cell wall, forming a mesh-like structure enwrapping the bacteria that is essential for maintaining structural integrity and providing support for anchoring other components of the cell envelope.
33536321	3	114	theme	glycosidic	497:506	arg1	bonds					517:521	glycosidic or amide bonds	497:521	glycosidic or amide bonds in the PG	497:531	PG biogenesis is highly dynamic and requires multiple enzymes, including several hydrolases that cleave glycosidic or amide bonds in the PG.
33536321	8	115	theme	Phdp	1153:1156	arg1	system					1176:1181	the Phdp type II secretion system	1149:1181	the Phdp type II secretion system	1149:1181	Interestingly, it is secreted by the Phdp type II secretion system and degrades the PG of Vibrio anguillarum and Vibrio vulnificus This suggests that PnpA is used by Phdp to gain an advantage over bacteria that compete for the same resources or to obtain nutrients in nutrient-scarce environments.
33536321	9	116	theme	mesh-like	1875:1883	arg1	structure					1885:1893	a mesh-like structure	1873:1893	a mesh-like structure that enwraps the bacterial cell	1873:1925	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	4	117	theme	damselae	657:664	arg1	subsp					666:670	Photobacterium damselae subsp	642:670	Photobacterium damselae subsp	642:670	This work describes the structural and functional characterization of an NlpC/P60-containing peptidase from Photobacterium damselae subsp.
33536321	8	118	from	nutrients	1371:1379	arg1	environments					1400:1411	nutrient-scarce environments	1384:1411	nutrient-scarce environments	1384:1411	Interestingly, it is secreted by the Phdp type II secretion system and degrades the PG of Vibrio anguillarum and Vibrio vulnificus This suggests that PnpA is used by Phdp to gain an advantage over bacteria that compete for the same resources or to obtain nutrients in nutrient-scarce environments.
33536321	9	119	from	muropeptides	1679:1690	arg1	PG					1728:1729	PG	1728:1729	PG	1728:1729	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
33536321	9	119	from	muropeptides	1679:1690	arg1	Peptidoglycan					1713:1725	the PG.IMPORTANCE Peptidoglycan	1695:1725	the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell	1695:1925	Comparison of the muropeptide composition of PG susceptible and resistant to the catalytic activity of PnpA showed that the global content of muropeptides is similar, suggesting that susceptibility to PnpA is determined by the three-dimensional organization of the muropeptides in the PG.IMPORTANCE Peptidoglycan (PG) is a major component of the bacterial cell wall formed by long chains of two alternating sugars interconnected by short peptides, generating a mesh-like structure that enwraps the bacterial cell.
32862508	7	0	theme	strategies	1461:1470	arg1	development					1446:1456	the development	1442:1456	the development of strategies that target virus-host surface glycan interactions to reduce MrNV infections	1442:1547	Therefore, the results from this study highly suggested that MrNV-VLPs preferentially attach to fucosylated N-glycans in the susceptible gill tissues, and these findings could lead to the development of strategies that target virus-host surface glycan interactions to reduce MrNV infections.
32862508	3	1	theme	susceptible	551:561	arg1	gills					583:587	the gills	579:587	the gills	579:587	Therefore, the present study investigated the role of glycans as binding molecules for virus attachment in susceptible tissues such as the gills.
32862508	3	1	theme	susceptible	551:561	arg1	tissues					563:569	susceptible tissues	551:569	susceptible tissues such as the gills	551:587	Therefore, the present study investigated the role of glycans as binding molecules for virus attachment in susceptible tissues such as the gills.
32862508	4	2	theme	particle	635:642	arg1	form					655:658	their virus-like particle (MrNV-VLP) form	618:658	their virus-like particle (MrNV-VLP) form	618:658	We established that MrNV in their virus-like particle (MrNV-VLP) form exhibited strong binding to gill tissues and lysates, which was highly reduced by the glycan-reducing periodate and PNGase F.
32862508	1	3	theme	many	230:233	arg1	species					235:241	many species	230:241	many species of shrimp and prawn	230:261	The Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white-tail disease (WTD) in many species of shrimp and prawn, has been shown to infect hemocytes and tissues such as the gills and muscles.
32862508	7	4	theme	gill	1395:1398	arg1	tissues					1400:1406	the susceptible gill tissues	1379:1406	the susceptible gill tissues	1379:1406	Therefore, the results from this study highly suggested that MrNV-VLPs preferentially attach to fucosylated N-glycans in the susceptible gill tissues, and these findings could lead to the development of strategies that target virus-host surface glycan interactions to reduce MrNV infections.
32862508	5	5	theme	Aurantia	820:827	arg1	AAL					837:839	AAL	837:839	AAL	837:839	The broad, fucose-binding Aleuria Aurantia lectin (AAL) highly reduced MrNV-VLPs binding to gill tissue sections and lysates, and efficiently disrupted the specific interactions between the VLPs and gill glycoproteins.
32862508	5	5	theme	Aurantia	820:827	arg1	lectin					829:834	The broad, fucose-binding Aleuria Aurantia lectin	786:834	lectin	829:834	The broad, fucose-binding Aleuria Aurantia lectin (AAL) highly reduced MrNV-VLPs binding to gill tissue sections and lysates, and efficiently disrupted the specific interactions between the VLPs and gill glycoproteins.
32862508	4	6	theme	virus-like	624:633	arg1	form					655:658	their virus-like particle (MrNV-VLP) form	618:658	their virus-like particle (MrNV-VLP) form	618:658	We established that MrNV in their virus-like particle (MrNV-VLP) form exhibited strong binding to gill tissues and lysates, which was highly reduced by the glycan-reducing periodate and PNGase F.
32862508	6	7	link	N-linked	1099:1106	arg1	glycans					1121:1127	unique fucosylated LacdiNAc-extended N-linked and O-linked glycans	1062:1127	unique fucosylated LacdiNAc-extended N-linked and O-linked glycans in the gill tissues	1062:1147	Furthermore, mass spectroscopy revealed the existence of unique fucosylated LacdiNAc-extended N-linked and O-linked glycans in the gill tissues, whereas beta-elimination experiments showed that MrNV-VLPs demonstrated a binding preference for N-glycans.
32862508	6	8	theme	mass	1018:1021	arg1	spectroscopy					1023:1034	mass spectroscopy	1018:1034	mass spectroscopy	1018:1034	Furthermore, mass spectroscopy revealed the existence of unique fucosylated LacdiNAc-extended N-linked and O-linked glycans in the gill tissues, whereas beta-elimination experiments showed that MrNV-VLPs demonstrated a binding preference for N-glycans.
32862508	2	9	attach	attach	414:419	arg1	molecules					390:398	the host surface molecules	373:398	the host surface molecules to which MrNV attach to initiate infection	373:441	However, little is known about the host surface molecules to which MrNV attach to initiate infection.
32862508	2	9	attach	attach	414:419	arg2	MrNV					409:412	MrNV	409:412	MrNV	409:412	However, little is known about the host surface molecules to which MrNV attach to initiate infection.
32862508	5	10	theme	gill	878:881	arg1	sections					890:897	gill tissue sections	878:897	gill tissue sections	878:897	The broad, fucose-binding Aleuria Aurantia lectin (AAL) highly reduced MrNV-VLPs binding to gill tissue sections and lysates, and efficiently disrupted the specific interactions between the VLPs and gill glycoproteins.
32862508	3	11	from	role	490:493	arg1	gills					583:587	the gills	579:587	the gills	579:587	Therefore, the present study investigated the role of glycans as binding molecules for virus attachment in susceptible tissues such as the gills.
32862508	3	11	from	role	490:493	arg1	tissues					563:569	susceptible tissues	551:569	susceptible tissues such as the gills	551:587	Therefore, the present study investigated the role of glycans as binding molecules for virus attachment in susceptible tissues such as the gills.
32862508	7	12	theme	MrNV	1533:1536	arg1	infections					1538:1547	MrNV infections	1533:1547	MrNV infections	1533:1547	Therefore, the results from this study highly suggested that MrNV-VLPs preferentially attach to fucosylated N-glycans in the susceptible gill tissues, and these findings could lead to the development of strategies that target virus-host surface glycan interactions to reduce MrNV infections.
32862508	7	13	theme	susceptible	1383:1393	arg1	tissues					1400:1406	the susceptible gill tissues	1379:1406	the susceptible gill tissues	1379:1406	Therefore, the results from this study highly suggested that MrNV-VLPs preferentially attach to fucosylated N-glycans in the susceptible gill tissues, and these findings could lead to the development of strategies that target virus-host surface glycan interactions to reduce MrNV infections.
32862508	6	14	theme	N-linked	1099:1106	arg1	glycans					1121:1127	unique fucosylated LacdiNAc-extended N-linked and O-linked glycans	1062:1127	unique fucosylated LacdiNAc-extended N-linked and O-linked glycans in the gill tissues	1062:1147	Furthermore, mass spectroscopy revealed the existence of unique fucosylated LacdiNAc-extended N-linked and O-linked glycans in the gill tissues, whereas beta-elimination experiments showed that MrNV-VLPs demonstrated a binding preference for N-glycans.
32862508	4	15	theme	gill	688:691	arg1	tissues					693:699	gill tissues	688:699	gill tissues	688:699	We established that MrNV in their virus-like particle (MrNV-VLP) form exhibited strong binding to gill tissues and lysates, which was highly reduced by the glycan-reducing periodate and PNGase F.
32862508	1	16	dep	Macrobrachium	135:147	arg1	rosenbergii					149:159	rosenbergii	149:159	rosenbergii	149:159	The Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white-tail disease (WTD) in many species of shrimp and prawn, has been shown to infect hemocytes and tissues such as the gills and muscles.
32862508	4	17	from	MrNV	610:613	arg1	form					655:658	their virus-like particle (MrNV-VLP) form	618:658	their virus-like particle (MrNV-VLP) form	618:658	We established that MrNV in their virus-like particle (MrNV-VLP) form exhibited strong binding to gill tissues and lysates, which was highly reduced by the glycan-reducing periodate and PNGase F.
32862508	1	18	theme	shrimp	246:251	arg1	species					235:241	many species	230:241	many species of shrimp and prawn	230:261	The Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white-tail disease (WTD) in many species of shrimp and prawn, has been shown to infect hemocytes and tissues such as the gills and muscles.
32862508	0	19	theme	freshwater	113:122	arg1	prawn					124:128	the giant freshwater prawn	103:128	the giant freshwater prawn	103:128	Macrobrachium rosenbergii nodavirus virus-like particles attach to fucosylated glycans in the gills of the giant freshwater prawn.
32862508	6	20	theme	unique	1062:1067	arg1	glycans					1121:1127	unique fucosylated LacdiNAc-extended N-linked and O-linked glycans	1062:1127	unique fucosylated LacdiNAc-extended N-linked and O-linked glycans in the gill tissues	1062:1147	Furthermore, mass spectroscopy revealed the existence of unique fucosylated LacdiNAc-extended N-linked and O-linked glycans in the gill tissues, whereas beta-elimination experiments showed that MrNV-VLPs demonstrated a binding preference for N-glycans.
32862508	5	21	theme	MrNV-VLPs	857:865	arg1	binding					867:873	MrNV-VLPs binding	857:873	MrNV-VLPs binding to gill tissue sections and lysates	857:909	The broad, fucose-binding Aleuria Aurantia lectin (AAL) highly reduced MrNV-VLPs binding to gill tissue sections and lysates, and efficiently disrupted the specific interactions between the VLPs and gill glycoproteins.
32862508	6	22	gly	fucosylated	1069:1079	arg1	glycans					1121:1127	unique fucosylated LacdiNAc-extended N-linked and O-linked glycans	1062:1127	unique fucosylated LacdiNAc-extended N-linked and O-linked glycans in the gill tissues	1062:1147	Furthermore, mass spectroscopy revealed the existence of unique fucosylated LacdiNAc-extended N-linked and O-linked glycans in the gill tissues, whereas beta-elimination experiments showed that MrNV-VLPs demonstrated a binding preference for N-glycans.
32862508	6	23	theme	glycans	1121:1127	arg1	existence					1049:1057	the existence	1045:1057	the existence of unique fucosylated LacdiNAc-extended N-linked and O-linked glycans in the gill tissues	1045:1147	Furthermore, mass spectroscopy revealed the existence of unique fucosylated LacdiNAc-extended N-linked and O-linked glycans in the gill tissues, whereas beta-elimination experiments showed that MrNV-VLPs demonstrated a binding preference for N-glycans.
32862508	0	24	theme	giant	107:111	arg1	prawn					124:128	the giant freshwater prawn	103:128	the giant freshwater prawn	103:128	Macrobrachium rosenbergii nodavirus virus-like particles attach to fucosylated glycans in the gills of the giant freshwater prawn.
32862508	0	25	theme	Macrobrachium	0:12	arg1	particles					47:55	Macrobrachium rosenbergii nodavirus virus-like particles	0:55	Macrobrachium rosenbergii nodavirus virus-like particles	0:55	Macrobrachium rosenbergii nodavirus virus-like particles attach to fucosylated glycans in the gills of the giant freshwater prawn.
32862508	4	26	dep	periodate	762:770	arg1	the					742:744	the	742:744	the	742:744	We established that MrNV in their virus-like particle (MrNV-VLP) form exhibited strong binding to gill tissues and lysates, which was highly reduced by the glycan-reducing periodate and PNGase F.
32862508	6	27	dep	revealed	1036:1043	arg1	whereas					1150:1156	whereas	1150:1156	whereas	1150:1156	Furthermore, mass spectroscopy revealed the existence of unique fucosylated LacdiNAc-extended N-linked and O-linked glycans in the gill tissues, whereas beta-elimination experiments showed that MrNV-VLPs demonstrated a binding preference for N-glycans.
32862508	0	28	attach	attach	57:62	arg1	glycans					79:85	fucosylated glycans	67:85	fucosylated glycans	67:85	Macrobrachium rosenbergii nodavirus virus-like particles attach to fucosylated glycans in the gills of the giant freshwater prawn.
32862508	0	28	attach	attach	57:62	arg2	particles					47:55	Macrobrachium rosenbergii nodavirus virus-like particles	0:55	Macrobrachium rosenbergii nodavirus virus-like particles	0:55	Macrobrachium rosenbergii nodavirus virus-like particles attach to fucosylated glycans in the gills of the giant freshwater prawn.
32862508	4	29	theme	strong	670:675	arg1	binding					677:683	strong binding	670:683	strong binding to gill tissues and lysates, which was highly reduced by the glycan-reducing periodate and PNGase F	670:783	We established that MrNV in their virus-like particle (MrNV-VLP) form exhibited strong binding to gill tissues and lysates, which was highly reduced by the glycan-reducing periodate and PNGase F.
32862508	5	30	theme	broad	790:794	arg1	AAL					837:839	AAL	837:839	AAL	837:839	The broad, fucose-binding Aleuria Aurantia lectin (AAL) highly reduced MrNV-VLPs binding to gill tissue sections and lysates, and efficiently disrupted the specific interactions between the VLPs and gill glycoproteins.
32862508	5	30	theme	broad	790:794	arg1	lectin					829:834	The broad, fucose-binding Aleuria Aurantia lectin	786:834	lectin	829:834	The broad, fucose-binding Aleuria Aurantia lectin (AAL) highly reduced MrNV-VLPs binding to gill tissue sections and lysates, and efficiently disrupted the specific interactions between the VLPs and gill glycoproteins.
32862508	2	31	theme	surface	382:388	arg1	molecules					390:398	the host surface molecules	373:398	the host surface molecules to which MrNV attach to initiate infection	373:441	However, little is known about the host surface molecules to which MrNV attach to initiate infection.
32862508	6	32	theme	binding	1224:1230	arg1	preference					1232:1241	a binding preference	1222:1241	a binding preference for N-glycans	1222:1255	Furthermore, mass spectroscopy revealed the existence of unique fucosylated LacdiNAc-extended N-linked and O-linked glycans in the gill tissues, whereas beta-elimination experiments showed that MrNV-VLPs demonstrated a binding preference for N-glycans.
32862508	4	33	theme	PNGase	776:781	arg1	F					783:783	PNGase F	776:783	PNGase F	776:783	We established that MrNV in their virus-like particle (MrNV-VLP) form exhibited strong binding to gill tissues and lysates, which was highly reduced by the glycan-reducing periodate and PNGase F.
32862508	3	34	theme	binding	509:515	arg1	molecules					517:525	binding molecules	509:525	binding molecules for virus attachment	509:546	Therefore, the present study investigated the role of glycans as binding molecules for virus attachment in susceptible tissues such as the gills.
32862508	1	35	theme	causative	183:191	arg1	nodavirus					161:169	The Macrobrachium rosenbergii nodavirus	131:169	The Macrobrachium rosenbergii nodavirus (MrNV)	131:176	The Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white-tail disease (WTD) in many species of shrimp and prawn, has been shown to infect hemocytes and tissues such as the gills and muscles.
32862508	1	35	theme	causative	183:191	arg1	agent					193:197	the causative agent	179:197	the causative agent of white-tail disease (WTD) in many species of shrimp and prawn	179:261	The Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white-tail disease (WTD) in many species of shrimp and prawn, has been shown to infect hemocytes and tissues such as the gills and muscles.
32862508	2	36	theme	host	377:380	arg1	molecules					390:398	the host surface molecules	373:398	the host surface molecules to which MrNV attach to initiate infection	373:441	However, little is known about the host surface molecules to which MrNV attach to initiate infection.
32862508	1	37	theme	prawn	257:261	arg1	species					235:241	many species	230:241	many species of shrimp and prawn	230:261	The Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white-tail disease (WTD) in many species of shrimp and prawn, has been shown to infect hemocytes and tissues such as the gills and muscles.
32862508	6	38	theme	LacdiNAc-extended	1081:1097	arg1	glycans					1121:1127	unique fucosylated LacdiNAc-extended N-linked and O-linked glycans	1062:1127	unique fucosylated LacdiNAc-extended N-linked and O-linked glycans in the gill tissues	1062:1147	Furthermore, mass spectroscopy revealed the existence of unique fucosylated LacdiNAc-extended N-linked and O-linked glycans in the gill tissues, whereas beta-elimination experiments showed that MrNV-VLPs demonstrated a binding preference for N-glycans.
32862508	7	39	theme	fucosylated	1354:1364	arg1	N-glycans					1366:1374	fucosylated N-glycans	1354:1374	fucosylated N-glycans	1354:1374	Therefore, the results from this study highly suggested that MrNV-VLPs preferentially attach to fucosylated N-glycans in the susceptible gill tissues, and these findings could lead to the development of strategies that target virus-host surface glycan interactions to reduce MrNV infections.
32862508	6	40	from	glycans	1121:1127	arg1	tissues					1141:1147	the gill tissues	1132:1147	the gill tissues	1132:1147	Furthermore, mass spectroscopy revealed the existence of unique fucosylated LacdiNAc-extended N-linked and O-linked glycans in the gill tissues, whereas beta-elimination experiments showed that MrNV-VLPs demonstrated a binding preference for N-glycans.
32862508	0	41	theme	nodavirus	26:34	arg1	particles					47:55	Macrobrachium rosenbergii nodavirus virus-like particles	0:55	Macrobrachium rosenbergii nodavirus virus-like particles	0:55	Macrobrachium rosenbergii nodavirus virus-like particles attach to fucosylated glycans in the gills of the giant freshwater prawn.
32862508	0	42	theme	prawn	124:128	arg1	gills					94:98	the gills	90:98	the gills of the giant freshwater prawn	90:128	Macrobrachium rosenbergii nodavirus virus-like particles attach to fucosylated glycans in the gills of the giant freshwater prawn.
32862508	5	43	theme	gill	985:988	arg1	glycoproteins					990:1002	gill glycoproteins	985:1002	gill glycoproteins	985:1002	The broad, fucose-binding Aleuria Aurantia lectin (AAL) highly reduced MrNV-VLPs binding to gill tissue sections and lysates, and efficiently disrupted the specific interactions between the VLPs and gill glycoproteins.
32862508	3	44	theme	glycans	498:504	arg1	role					490:493	the role	486:493	the role of glycans as binding molecules for virus attachment in susceptible tissues such as the gills	486:587	Therefore, the present study investigated the role of glycans as binding molecules for virus attachment in susceptible tissues such as the gills.
32862508	5	45	theme	specific	942:949	arg1	interactions					951:962	the specific interactions	938:962	the specific interactions between the VLPs and gill glycoproteins	938:1002	The broad, fucose-binding Aleuria Aurantia lectin (AAL) highly reduced MrNV-VLPs binding to gill tissue sections and lysates, and efficiently disrupted the specific interactions between the VLPs and gill glycoproteins.
32862508	5	46	theme	tissue	883:888	arg1	sections					890:897	gill tissue sections	878:897	gill tissue sections	878:897	The broad, fucose-binding Aleuria Aurantia lectin (AAL) highly reduced MrNV-VLPs binding to gill tissue sections and lysates, and efficiently disrupted the specific interactions between the VLPs and gill glycoproteins.
32862508	3	47	theme	virus	531:535	arg1	attachment					537:546	virus attachment	531:546	virus attachment	531:546	Therefore, the present study investigated the role of glycans as binding molecules for virus attachment in susceptible tissues such as the gills.
32862508	6	48	theme	gill	1136:1139	arg1	tissues					1141:1147	the gill tissues	1132:1147	the gill tissues	1132:1147	Furthermore, mass spectroscopy revealed the existence of unique fucosylated LacdiNAc-extended N-linked and O-linked glycans in the gill tissues, whereas beta-elimination experiments showed that MrNV-VLPs demonstrated a binding preference for N-glycans.
32862508	1	49	theme	white-tail	202:211	arg1	WTD					222:224	WTD	222:224	WTD	222:224	The Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white-tail disease (WTD) in many species of shrimp and prawn, has been shown to infect hemocytes and tissues such as the gills and muscles.
32862508	1	49	theme	white-tail	202:211	arg1	disease					213:219	white-tail disease	202:219	white-tail disease (WTD)	202:225	The Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white-tail disease (WTD) in many species of shrimp and prawn, has been shown to infect hemocytes and tissues such as the gills and muscles.
32862508	7	50	gly	fucosylated	1354:1364	arg1	N-glycans					1366:1374	fucosylated N-glycans	1354:1374	fucosylated N-glycans	1354:1374	Therefore, the results from this study highly suggested that MrNV-VLPs preferentially attach to fucosylated N-glycans in the susceptible gill tissues, and these findings could lead to the development of strategies that target virus-host surface glycan interactions to reduce MrNV infections.
32862508	7	51	theme	glycan	1503:1508	arg1	interactions					1510:1521	virus-host surface glycan interactions	1484:1521	virus-host surface glycan interactions	1484:1521	Therefore, the results from this study highly suggested that MrNV-VLPs preferentially attach to fucosylated N-glycans in the susceptible gill tissues, and these findings could lead to the development of strategies that target virus-host surface glycan interactions to reduce MrNV infections.
32862508	0	52	theme	virus-like	36:45	arg1	particles					47:55	Macrobrachium rosenbergii nodavirus virus-like particles	0:55	Macrobrachium rosenbergii nodavirus virus-like particles	0:55	Macrobrachium rosenbergii nodavirus virus-like particles attach to fucosylated glycans in the gills of the giant freshwater prawn.
32862508	6	53	from	existence	1049:1057	arg1	tissues					1141:1147	the gill tissues	1132:1147	the gill tissues	1132:1147	Furthermore, mass spectroscopy revealed the existence of unique fucosylated LacdiNAc-extended N-linked and O-linked glycans in the gill tissues, whereas beta-elimination experiments showed that MrNV-VLPs demonstrated a binding preference for N-glycans.
32862508	1	54	theme	disease	213:219	arg1	nodavirus					161:169	The Macrobrachium rosenbergii nodavirus	131:169	The Macrobrachium rosenbergii nodavirus (MrNV)	131:176	The Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white-tail disease (WTD) in many species of shrimp and prawn, has been shown to infect hemocytes and tissues such as the gills and muscles.
32862508	1	54	theme	disease	213:219	arg1	agent					193:197	the causative agent	179:197	the causative agent of white-tail disease (WTD) in many species of shrimp and prawn	179:261	The Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white-tail disease (WTD) in many species of shrimp and prawn, has been shown to infect hemocytes and tissues such as the gills and muscles.
32862508	0	55	theme	fucosylated	67:77	arg1	glycans					79:85	fucosylated glycans	67:85	fucosylated glycans	67:85	Macrobrachium rosenbergii nodavirus virus-like particles attach to fucosylated glycans in the gills of the giant freshwater prawn.
32862508	5	56	theme	fucose-binding	797:810	arg1	AAL					837:839	AAL	837:839	AAL	837:839	The broad, fucose-binding Aleuria Aurantia lectin (AAL) highly reduced MrNV-VLPs binding to gill tissue sections and lysates, and efficiently disrupted the specific interactions between the VLPs and gill glycoproteins.
32862508	5	56	theme	fucose-binding	797:810	arg1	lectin					829:834	The broad, fucose-binding Aleuria Aurantia lectin	786:834	lectin	829:834	The broad, fucose-binding Aleuria Aurantia lectin (AAL) highly reduced MrNV-VLPs binding to gill tissue sections and lysates, and efficiently disrupted the specific interactions between the VLPs and gill glycoproteins.
32862508	4	57	theme	glycan-reducing	746:760	arg1	periodate					762:770	glycan-reducing periodate	746:770	glycan-reducing periodate	746:770	We established that MrNV in their virus-like particle (MrNV-VLP) form exhibited strong binding to gill tissues and lysates, which was highly reduced by the glycan-reducing periodate and PNGase F.
32862508	4	58	theme	MrNV-VLP	645:652	arg1	form					655:658	their virus-like particle (MrNV-VLP) form	618:658	their virus-like particle (MrNV-VLP) form	618:658	We established that MrNV in their virus-like particle (MrNV-VLP) form exhibited strong binding to gill tissues and lysates, which was highly reduced by the glycan-reducing periodate and PNGase F.
32862508	7	59	theme	virus-host	1484:1493	arg1	interactions					1510:1521	virus-host surface glycan interactions	1484:1521	virus-host surface glycan interactions	1484:1521	Therefore, the results from this study highly suggested that MrNV-VLPs preferentially attach to fucosylated N-glycans in the susceptible gill tissues, and these findings could lead to the development of strategies that target virus-host surface glycan interactions to reduce MrNV infections.
32862508	6	60	theme	beta-elimination	1158:1173	arg1	experiments					1175:1185	beta-elimination experiments	1158:1185	beta-elimination experiments	1158:1185	Furthermore, mass spectroscopy revealed the existence of unique fucosylated LacdiNAc-extended N-linked and O-linked glycans in the gill tissues, whereas beta-elimination experiments showed that MrNV-VLPs demonstrated a binding preference for N-glycans.
32862508	1	61	from	agent	193:197	arg1	species					235:241	many species	230:241	many species of shrimp and prawn	230:261	The Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white-tail disease (WTD) in many species of shrimp and prawn, has been shown to infect hemocytes and tissues such as the gills and muscles.
32862508	5	62	gly	glycoproteins	990:1002	arg1	glycoproteins					990:1002	gill glycoproteins	985:1002	gill glycoproteins	985:1002	The broad, fucose-binding Aleuria Aurantia lectin (AAL) highly reduced MrNV-VLPs binding to gill tissue sections and lysates, and efficiently disrupted the specific interactions between the VLPs and gill glycoproteins.
32862508	0	63	gly	fucosylated	67:77	arg1	glycans					79:85	fucosylated glycans	67:85	fucosylated glycans	67:85	Macrobrachium rosenbergii nodavirus virus-like particles attach to fucosylated glycans in the gills of the giant freshwater prawn.
32862508	7	64	theme	surface	1495:1501	arg1	interactions					1510:1521	virus-host surface glycan interactions	1484:1521	virus-host surface glycan interactions	1484:1521	Therefore, the results from this study highly suggested that MrNV-VLPs preferentially attach to fucosylated N-glycans in the susceptible gill tissues, and these findings could lead to the development of strategies that target virus-host surface glycan interactions to reduce MrNV infections.
32862508	6	65	theme	O-linked	1112:1119	arg1	glycans					1121:1127	unique fucosylated LacdiNAc-extended N-linked and O-linked glycans	1062:1127	unique fucosylated LacdiNAc-extended N-linked and O-linked glycans in the gill tissues	1062:1147	Furthermore, mass spectroscopy revealed the existence of unique fucosylated LacdiNAc-extended N-linked and O-linked glycans in the gill tissues, whereas beta-elimination experiments showed that MrNV-VLPs demonstrated a binding preference for N-glycans.
32862508	1	66	theme	Macrobrachium	135:147	arg1	nodavirus					161:169	The Macrobrachium rosenbergii nodavirus	131:169	The Macrobrachium rosenbergii nodavirus (MrNV)	131:176	The Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white-tail disease (WTD) in many species of shrimp and prawn, has been shown to infect hemocytes and tissues such as the gills and muscles.
32862508	1	66	theme	Macrobrachium	135:147	arg1	agent					193:197	the causative agent	179:197	the causative agent of white-tail disease (WTD) in many species of shrimp and prawn	179:261	The Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white-tail disease (WTD) in many species of shrimp and prawn, has been shown to infect hemocytes and tissues such as the gills and muscles.
32862508	1	66	theme	Macrobrachium	135:147	arg1	MrNV					172:175	MrNV	172:175	MrNV	172:175	The Macrobrachium rosenbergii nodavirus (MrNV), the causative agent of white-tail disease (WTD) in many species of shrimp and prawn, has been shown to infect hemocytes and tissues such as the gills and muscles.
32862508	7	67	attach	attach	1344:1349	arg1	N-glycans					1366:1374	fucosylated N-glycans	1354:1374	fucosylated N-glycans	1354:1374	Therefore, the results from this study highly suggested that MrNV-VLPs preferentially attach to fucosylated N-glycans in the susceptible gill tissues, and these findings could lead to the development of strategies that target virus-host surface glycan interactions to reduce MrNV infections.
32862508	7	67	attach	attach	1344:1349	arg2	MrNV-VLPs					1319:1327	MrNV-VLPs	1319:1327	MrNV-VLPs	1319:1327	Therefore, the results from this study highly suggested that MrNV-VLPs preferentially attach to fucosylated N-glycans in the susceptible gill tissues, and these findings could lead to the development of strategies that target virus-host surface glycan interactions to reduce MrNV infections.
32862508	6	68	link	O-linked	1112:1119	arg1	glycans					1121:1127	unique fucosylated LacdiNAc-extended N-linked and O-linked glycans	1062:1127	unique fucosylated LacdiNAc-extended N-linked and O-linked glycans in the gill tissues	1062:1147	Furthermore, mass spectroscopy revealed the existence of unique fucosylated LacdiNAc-extended N-linked and O-linked glycans in the gill tissues, whereas beta-elimination experiments showed that MrNV-VLPs demonstrated a binding preference for N-glycans.
32862508	5	69	theme	Aleuria	812:818	arg1	AAL					837:839	AAL	837:839	AAL	837:839	The broad, fucose-binding Aleuria Aurantia lectin (AAL) highly reduced MrNV-VLPs binding to gill tissue sections and lysates, and efficiently disrupted the specific interactions between the VLPs and gill glycoproteins.
32862508	5	69	theme	Aleuria	812:818	arg1	lectin					829:834	The broad, fucose-binding Aleuria Aurantia lectin	786:834	lectin	829:834	The broad, fucose-binding Aleuria Aurantia lectin (AAL) highly reduced MrNV-VLPs binding to gill tissue sections and lysates, and efficiently disrupted the specific interactions between the VLPs and gill glycoproteins.
32862508	6	70	theme	fucosylated	1069:1079	arg1	glycans					1121:1127	unique fucosylated LacdiNAc-extended N-linked and O-linked glycans	1062:1127	unique fucosylated LacdiNAc-extended N-linked and O-linked glycans in the gill tissues	1062:1147	Furthermore, mass spectroscopy revealed the existence of unique fucosylated LacdiNAc-extended N-linked and O-linked glycans in the gill tissues, whereas beta-elimination experiments showed that MrNV-VLPs demonstrated a binding preference for N-glycans.
32862508	0	71	dep	Macrobrachium	0:12	arg1	rosenbergii					14:24	rosenbergii	14:24	rosenbergii	14:24	Macrobrachium rosenbergii nodavirus virus-like particles attach to fucosylated glycans in the gills of the giant freshwater prawn.
32862508	7	72	from	study	1291:1295	arg1	results					1273:1279	the results	1269:1279	the results from this study	1269:1295	Therefore, the results from this study highly suggested that MrNV-VLPs preferentially attach to fucosylated N-glycans in the susceptible gill tissues, and these findings could lead to the development of strategies that target virus-host surface glycan interactions to reduce MrNV infections.
32862508	3	73	theme	present	459:465	arg1	study					467:471	the present study	455:471	the present study	455:471	Therefore, the present study investigated the role of glycans as binding molecules for virus attachment in susceptible tissues such as the gills.
32862508	6	74	from	tissues	1141:1147	arg1	existence					1049:1057	the existence	1045:1057	the existence of unique fucosylated LacdiNAc-extended N-linked and O-linked glycans in the gill tissues	1045:1147	Furthermore, mass spectroscopy revealed the existence of unique fucosylated LacdiNAc-extended N-linked and O-linked glycans in the gill tissues, whereas beta-elimination experiments showed that MrNV-VLPs demonstrated a binding preference for N-glycans.
32359163	10	0	from	kingdoms	1925:1932	arg1	regions					1888:1894	the abundant disordered regions	1864:1894	the abundant disordered regions in protein families from all kingdoms of life	1864:1940	These findings will inform research on the abundant disordered regions in protein families from all kingdoms of life.
32359163	10	0	from	kingdoms	1925:1932	arg1	families					1907:1914	protein families	1899:1914	protein families from all kingdoms of life	1899:1940	These findings will inform research on the abundant disordered regions in protein families from all kingdoms of life.
32359163	4	1	theme	several	873:879	arg1	types					890:894	several distinct types	873:894	a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions	814:997	We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.
32359163	3	2	theme	new	524:526	arg1	approaches					539:548	new analytical approaches	524:548	new analytical approaches that project representations of sequence space	524:595	Here, we overcome these limitations by using new analytical approaches that project representations of sequence space to dissect the evolution of proteins with both ordered and disordered regions, and the correlated changes between these.
32359163	7	3	gly	glycosylation	1456:1468	arg2	motifs					1470:1475	the N-linked and O-linked glycosylation motifs	1430:1475	the N-linked and O-linked glycosylation motifs	1430:1475	Sequence features of the globular and disordered regions clearly correlate with one another, implying coevolution of these distinct regions, as well as with the N-linked and O-linked glycosylation motifs.
32359163	7	4	theme	O-linked	1447:1454	arg1	motifs					1470:1475	the N-linked and O-linked glycosylation motifs	1430:1475	the N-linked and O-linked glycosylation motifs	1430:1475	Sequence features of the globular and disordered regions clearly correlate with one another, implying coevolution of these distinct regions, as well as with the N-linked and O-linked glycosylation motifs.
32359163	8	5	theme	region	1659:1664	arg1	features					1675:1682	disordered region sequence features	1648:1682	disordered region sequence features	1648:1682	We reconstruct the overall evolutionary history of the FLAs, annotated with the changing domain architectures, glycosylation motifs, number and length of AG regions, and disordered region sequence features.
32359163	2	6	theme	disordered	281:290	arg1	regions					292:298	disordered regions	281:298	disordered regions	281:298	Our understanding of the evolution of proteins containing disordered regions therefore lags that of globular proteins, limiting our capacity to estimate their evolutionary history, classify paralogs, and identify potential sequence-function relationships.
32359163	4	7	theme	domains	854:860	arg1	number					825:830	a variable number	814:830	a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions	814:997	We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.
32359163	4	7	theme	domains	854:860	arg1	types					890:894	several distinct types	873:894	a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions	814:997	We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.
32359163	7	8	theme	N-linked	1434:1441	arg1	motifs					1470:1475	the N-linked and O-linked glycosylation motifs	1430:1475	the N-linked and O-linked glycosylation motifs	1430:1475	Sequence features of the globular and disordered regions clearly correlate with one another, implying coevolution of these distinct regions, as well as with the N-linked and O-linked glycosylation motifs.
32359163	2	9	theme	proteins	261:268	arg1	evolution					248:256	the evolution	244:256	the evolution of proteins containing disordered regions	244:298	Our understanding of the evolution of proteins containing disordered regions therefore lags that of globular proteins, limiting our capacity to estimate their evolutionary history, classify paralogs, and identify potential sequence-function relationships.
32359163	3	10	theme	space	591:595	arg1	representations					563:577	project representations	555:577	project representations of sequence space	555:595	Here, we overcome these limitations by using new analytical approaches that project representations of sequence space to dissect the evolution of proteins with both ordered and disordered regions, and the correlated changes between these.
32359163	6	11	theme	disordered	1253:1262	arg1	regions					1264:1270	Pro-depleted disordered regions	1240:1270	Pro-depleted disordered regions	1240:1270	Clusters can be similarly identified in the seemingly random Pro-rich AG and Pro-depleted disordered regions.
32359163	3	12	theme	proteins	625:632	arg1	changes					695:701	the correlated changes	680:701	the correlated changes between these	680:715	Here, we overcome these limitations by using new analytical approaches that project representations of sequence space to dissect the evolution of proteins with both ordered and disordered regions, and the correlated changes between these.
32359163	3	12	theme	proteins	625:632	arg1	evolution					612:620	the evolution	608:620	the evolution of proteins with both ordered and disordered regions	608:673	Here, we overcome these limitations by using new analytical approaches that project representations of sequence space to dissect the evolution of proteins with both ordered and disordered regions, and the correlated changes between these.
32359163	1	13	theme	disordered	201:210	arg1	sequences					212:220	disordered sequences	201:220	disordered sequences	201:220	Approaches for studying the evolution of globular proteins are now well established yet are unsuitable for disordered sequences.
32359163	4	14	theme	disordered	899:908	arg1	regions					910:916	disordered regions	899:916	disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions	899:997	We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.
32359163	7	15	theme	distinct	1396:1403	arg1	regions					1405:1411	these distinct regions	1390:1411	these distinct regions	1390:1411	Sequence features of the globular and disordered regions clearly correlate with one another, implying coevolution of these distinct regions, as well as with the N-linked and O-linked glycosylation motifs.
32359163	4	16	theme	fasciclin-like	729:742	arg1	family					787:792	a model family	779:792	a model family	779:792	We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.
32359163	4	16	theme	fasciclin-like	729:742	arg1	FLAs					770:773	FLAs	770:773	FLAs	770:773	We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.
32359163	4	16	theme	fasciclin-like	729:742	arg1	proteins					760:767	the fasciclin-like arabinogalactan proteins	725:767	the fasciclin-like arabinogalactan proteins (FLAs)	725:774	We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.
32359163	5	17	theme	domains	1040:1046	arg1	projections					1015:1025	Sequence space projections	1000:1025	Sequence space projections of fasciclin domains from 2019 FLAs from 78 species	1000:1077	Sequence space projections of fasciclin domains from 2019 FLAs from 78 species identified distinct clusters corresponding to different types of fasciclin domains.
32359163	9	18	theme	sequence-function	1773:1789	arg1	relationships					1791:1803	their sequence-function relationships	1767:1803	their sequence-function relationships	1767:1803	Mapping these features onto the functionally characterized FLAs therefore enables their sequence-function relationships to be interrogated.
32359163	6	19	theme	Pro-rich	1224:1231	arg1	AG					1233:1234	the seemingly random Pro-rich AG	1203:1234	the seemingly random Pro-rich AG	1203:1234	Clusters can be similarly identified in the seemingly random Pro-rich AG and Pro-depleted disordered regions.
32359163	4	20	contain	contain	806:812	arg2	types					890:894	several distinct types	873:894	a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions	814:997	We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.
32359163	4	20	contain	contain	806:812	arg2	number					825:830	a variable number	814:830	a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions	814:997	We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.
32359163	4	20	contain	contain	806:812	arg1	they					801:804	they	801:804	they	801:804	We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.
32359163	5	21	theme	2019	1053:1056	arg1	FLAs					1058:1061	2019 FLAs	1053:1061	2019 FLAs from 78 species	1053:1077	Sequence space projections of fasciclin domains from 2019 FLAs from 78 species identified distinct clusters corresponding to different types of fasciclin domains.
32359163	2	22	theme	evolution	248:256	arg1	understanding					227:239	Our understanding	223:239	Our understanding of the evolution of proteins containing disordered regions	223:298	Our understanding of the evolution of proteins containing disordered regions therefore lags that of globular proteins, limiting our capacity to estimate their evolutionary history, classify paralogs, and identify potential sequence-function relationships.
32359163	8	23	theme	evolutionary	1505:1516	arg1	history					1518:1524	the overall evolutionary history	1493:1524	the overall evolutionary history	1493:1524	We reconstruct the overall evolutionary history of the FLAs, annotated with the changing domain architectures, glycosylation motifs, number and length of AG regions, and disordered region sequence features.
32359163	7	24	with	coevolution	1375:1385	arg1	motifs					1470:1475	the N-linked and O-linked glycosylation motifs	1430:1475	the N-linked and O-linked glycosylation motifs	1430:1475	Sequence features of the globular and disordered regions clearly correlate with one another, implying coevolution of these distinct regions, as well as with the N-linked and O-linked glycosylation motifs.
32359163	5	25	theme	different	1125:1133	arg1	types					1135:1139	different types	1125:1139	different types of fasciclin domains	1125:1160	Sequence space projections of fasciclin domains from 2019 FLAs from 78 species identified distinct clusters corresponding to different types of fasciclin domains.
32359163	10	26	theme	disordered	1877:1886	arg1	regions					1888:1894	the abundant disordered regions	1864:1894	the abundant disordered regions in protein families from all kingdoms of life	1864:1940	These findings will inform research on the abundant disordered regions in protein families from all kingdoms of life.
32359163	1	27	theme	globular	135:142	arg1	proteins					144:151	globular proteins	135:151	globular proteins	135:151	Approaches for studying the evolution of globular proteins are now well established yet are unsuitable for disordered sequences.
32359163	4	28	theme	longer	971:976	arg1	regions					991:997	longer Pro-depleted regions	971:997	longer Pro-depleted regions	971:997	We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.
32359163	4	29	theme	arabinogalactan	938:952	arg1	regions					959:965	proline (Pro)-rich arabinogalactan (AG) regions	919:965	proline (Pro)-rich arabinogalactan (AG) regions	919:965	We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.
32359163	8	30	theme	FLAs	1533:1536	arg1	history					1518:1524	the overall evolutionary history	1493:1524	the overall evolutionary history	1493:1524	We reconstruct the overall evolutionary history of the FLAs, annotated with the changing domain architectures, glycosylation motifs, number and length of AG regions, and disordered region sequence features.
32359163	4	31	theme	model	781:785	arg1	family					787:792	a model family	779:792	a model family	779:792	We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.
32359163	4	31	theme	model	781:785	arg1	proteins					760:767	the fasciclin-like arabinogalactan proteins	725:767	the fasciclin-like arabinogalactan proteins (FLAs)	725:774	We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.
32359163	5	32	theme	distinct	1090:1097	arg1	clusters					1099:1106	distinct clusters	1090:1106	distinct clusters corresponding to different types of fasciclin domains	1090:1160	Sequence space projections of fasciclin domains from 2019 FLAs from 78 species identified distinct clusters corresponding to different types of fasciclin domains.
32359163	7	33	link	O-linked	1447:1454	arg1	motifs					1470:1475	the N-linked and O-linked glycosylation motifs	1430:1475	the N-linked and O-linked glycosylation motifs	1430:1475	Sequence features of the globular and disordered regions clearly correlate with one another, implying coevolution of these distinct regions, as well as with the N-linked and O-linked glycosylation motifs.
32359163	3	34	with	proteins	625:632	arg1	ordered					644:650	ordered	644:650	ordered	644:650	Here, we overcome these limitations by using new analytical approaches that project representations of sequence space to dissect the evolution of proteins with both ordered and disordered regions, and the correlated changes between these.
32359163	3	34	with	proteins	625:632	arg1	regions					667:673	disordered regions	656:673	disordered regions	656:673	Here, we overcome these limitations by using new analytical approaches that project representations of sequence space to dissect the evolution of proteins with both ordered and disordered regions, and the correlated changes between these.
32359163	8	35	gly	glycosylation	1589:1601	arg2	motifs					1603:1608	glycosylation motifs	1589:1608	glycosylation motifs	1589:1608	We reconstruct the overall evolutionary history of the FLAs, annotated with the changing domain architectures, glycosylation motifs, number and length of AG regions, and disordered region sequence features.
32359163	2	36	theme	evolutionary	382:393	arg1	history					395:401	their evolutionary history	376:401	their evolutionary history	376:401	Our understanding of the evolution of proteins containing disordered regions therefore lags that of globular proteins, limiting our capacity to estimate their evolutionary history, classify paralogs, and identify potential sequence-function relationships.
32359163	10	37	from	regions	1888:1894	arg1	families					1907:1914	protein families	1899:1914	protein families from all kingdoms of life	1899:1940	These findings will inform research on the abundant disordered regions in protein families from all kingdoms of life.
32359163	10	37	from	regions	1888:1894	arg1	kingdoms					1925:1932	all kingdoms	1921:1932	all kingdoms of life	1921:1940	These findings will inform research on the abundant disordered regions in protein families from all kingdoms of life.
32359163	2	38	theme	potential	436:444	arg1	relationships					464:476	potential sequence-function relationships	436:476	potential sequence-function relationships	436:476	Our understanding of the evolution of proteins containing disordered regions therefore lags that of globular proteins, limiting our capacity to estimate their evolutionary history, classify paralogs, and identify potential sequence-function relationships.
32359163	8	39	theme	domain	1567:1572	arg1	architectures					1574:1586	the changing domain architectures	1554:1586	the changing domain architectures	1554:1586	We reconstruct the overall evolutionary history of the FLAs, annotated with the changing domain architectures, glycosylation motifs, number and length of AG regions, and disordered region sequence features.
32359163	0	40	theme	Sequence-Diverse	13:28	arg1	Regions					41:47	Sequence-Diverse Disordered Regions	13:47	Sequence-Diverse Disordered Regions	13:47	Evolution of Sequence-Diverse Disordered Regions in a Protein Family: Order within the Chaos.
32359163	0	41	theme	Regions	41:47	arg1	Evolution					0:8	Evolution	0:8	Evolution of Sequence-Diverse Disordered Regions in a Protein Family: Order within the Chaos.	0:92	Evolution of Sequence-Diverse Disordered Regions in a Protein Family: Order within the Chaos.
32359163	5	42	theme	domains	1154:1160	arg1	types					1135:1139	different types	1125:1139	different types of fasciclin domains	1125:1160	Sequence space projections of fasciclin domains from 2019 FLAs from 78 species identified distinct clusters corresponding to different types of fasciclin domains.
32359163	7	43	dep	one	1353:1355	arg1	another					1357:1363	another	1357:1363	another	1357:1363	Sequence features of the globular and disordered regions clearly correlate with one another, implying coevolution of these distinct regions, as well as with the N-linked and O-linked glycosylation motifs.
32359163	7	44	theme	regions	1322:1328	arg1	features					1282:1289	Sequence features	1273:1289	Sequence features of the globular and disordered regions	1273:1328	Sequence features of the globular and disordered regions clearly correlate with one another, implying coevolution of these distinct regions, as well as with the N-linked and O-linked glycosylation motifs.
32359163	5	45	from	FLAs	1058:1061	arg1	projections					1015:1025	Sequence space projections	1000:1025	Sequence space projections of fasciclin domains from 2019 FLAs from 78 species	1000:1077	Sequence space projections of fasciclin domains from 2019 FLAs from 78 species identified distinct clusters corresponding to different types of fasciclin domains.
32359163	4	46	theme	globular	835:842	arg1	domains					854:860	globular fasciclin domains	835:860	globular fasciclin domains	835:860	We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.
32359163	5	47	theme	fasciclin	1030:1038	arg1	domains					1040:1046	fasciclin domains	1030:1046	fasciclin domains	1030:1046	Sequence space projections of fasciclin domains from 2019 FLAs from 78 species identified distinct clusters corresponding to different types of fasciclin domains.
32359163	8	48	theme	sequence	1666:1673	arg1	features					1675:1682	disordered region sequence features	1648:1682	disordered region sequence features	1648:1682	We reconstruct the overall evolutionary history of the FLAs, annotated with the changing domain architectures, glycosylation motifs, number and length of AG regions, and disordered region sequence features.
32359163	3	49	theme	project	555:561	arg1	representations					563:577	project representations	555:577	project representations of sequence space	555:595	Here, we overcome these limitations by using new analytical approaches that project representations of sequence space to dissect the evolution of proteins with both ordered and disordered regions, and the correlated changes between these.
32359163	3	50	theme	correlated	684:693	arg1	changes					695:701	the correlated changes	680:701	the correlated changes between these	680:715	Here, we overcome these limitations by using new analytical approaches that project representations of sequence space to dissect the evolution of proteins with both ordered and disordered regions, and the correlated changes between these.
32359163	3	51	theme	analytical	528:537	arg1	approaches					539:548	new analytical approaches	524:548	new analytical approaches that project representations of sequence space	524:595	Here, we overcome these limitations by using new analytical approaches that project representations of sequence space to dissect the evolution of proteins with both ordered and disordered regions, and the correlated changes between these.
32359163	5	52	theme	Sequence	1000:1007	arg1	space					1009:1013	Sequence space	1000:1013	Sequence space projections of fasciclin domains from 2019 FLAs from 78 species	1000:1077	Sequence space projections of fasciclin domains from 2019 FLAs from 78 species identified distinct clusters corresponding to different types of fasciclin domains.
32359163	8	53	theme	AG	1632:1633	arg1	regions					1635:1641	AG regions	1632:1641	AG regions	1632:1641	We reconstruct the overall evolutionary history of the FLAs, annotated with the changing domain architectures, glycosylation motifs, number and length of AG regions, and disordered region sequence features.
32359163	4	54	theme	distinct	881:888	arg1	types					890:894	several distinct types	873:894	a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions	814:997	We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.
32359163	7	55	theme	glycosylation	1456:1468	arg1	motifs					1470:1475	the N-linked and O-linked glycosylation motifs	1430:1475	the N-linked and O-linked glycosylation motifs	1430:1475	Sequence features of the globular and disordered regions clearly correlate with one another, implying coevolution of these distinct regions, as well as with the N-linked and O-linked glycosylation motifs.
32359163	8	56	theme	disordered	1648:1657	arg1	features					1675:1682	disordered region sequence features	1648:1682	disordered region sequence features	1648:1682	We reconstruct the overall evolutionary history of the FLAs, annotated with the changing domain architectures, glycosylation motifs, number and length of AG regions, and disordered region sequence features.
32359163	4	57	theme	fasciclin	844:852	arg1	domains					854:860	globular fasciclin domains	835:860	globular fasciclin domains	835:860	We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.
32359163	4	58	dep	regions	910:916	arg1	regions					959:965	proline (Pro)-rich arabinogalactan (AG) regions	919:965	proline (Pro)-rich arabinogalactan (AG) regions	919:965	We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.
32359163	4	58	dep	regions	910:916	arg1	regions					991:997	longer Pro-depleted regions	971:997	longer Pro-depleted regions	971:997	We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.
32359163	3	59	theme	sequence	582:589	arg1	space					591:595	sequence space	582:595	sequence space	582:595	Here, we overcome these limitations by using new analytical approaches that project representations of sequence space to dissect the evolution of proteins with both ordered and disordered regions, and the correlated changes between these.
32359163	9	60	theme	characterized	1730:1742	arg1	FLAs					1744:1747	the functionally characterized FLAs	1713:1747	the functionally characterized FLAs	1713:1747	Mapping these features onto the functionally characterized FLAs therefore enables their sequence-function relationships to be interrogated.
32359163	10	61	theme	abundant	1868:1875	arg1	regions					1888:1894	the abundant disordered regions	1864:1894	the abundant disordered regions in protein families from all kingdoms of life	1864:1940	These findings will inform research on the abundant disordered regions in protein families from all kingdoms of life.
32359163	6	62	theme	Pro-depleted	1240:1251	arg1	regions					1264:1270	Pro-depleted disordered regions	1240:1270	Pro-depleted disordered regions	1240:1270	Clusters can be similarly identified in the seemingly random Pro-rich AG and Pro-depleted disordered regions.
32359163	7	63	link	N-linked	1434:1441	arg1	motifs					1470:1475	the N-linked and O-linked glycosylation motifs	1430:1475	the N-linked and O-linked glycosylation motifs	1430:1475	Sequence features of the globular and disordered regions clearly correlate with one another, implying coevolution of these distinct regions, as well as with the N-linked and O-linked glycosylation motifs.
32359163	0	64	from	Evolution	0:8	arg1	Family					62:67	a Protein Family	52:67	a Protein Family	52:67	Evolution of Sequence-Diverse Disordered Regions in a Protein Family: Order within the Chaos.
32359163	8	65	theme	overall	1497:1503	arg1	history					1518:1524	the overall evolutionary history	1493:1524	the overall evolutionary history	1493:1524	We reconstruct the overall evolutionary history of the FLAs, annotated with the changing domain architectures, glycosylation motifs, number and length of AG regions, and disordered region sequence features.
32359163	7	66	theme	regions	1405:1411	arg1	coevolution					1375:1385	coevolution	1375:1385	coevolution of these distinct regions, as well as with the N-linked and O-linked glycosylation motifs	1375:1475	Sequence features of the globular and disordered regions clearly correlate with one another, implying coevolution of these distinct regions, as well as with the N-linked and O-linked glycosylation motifs.
32359163	10	67	theme	protein	1899:1905	arg1	families					1907:1914	protein families	1899:1914	protein families from all kingdoms of life	1899:1940	These findings will inform research on the abundant disordered regions in protein families from all kingdoms of life.
32359163	4	68	theme	regions	910:916	arg1	number					825:830	a variable number	814:830	a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions	814:997	We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.
32359163	4	68	theme	regions	910:916	arg1	types					890:894	several distinct types	873:894	a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions	814:997	We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.
32359163	6	69	theme	random	1217:1222	arg1	AG					1233:1234	the seemingly random Pro-rich AG	1203:1234	the seemingly random Pro-rich AG	1203:1234	Clusters can be similarly identified in the seemingly random Pro-rich AG and Pro-depleted disordered regions.
32359163	4	70	theme	arabinogalactan	744:758	arg1	family					787:792	a model family	779:792	a model family	779:792	We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.
32359163	4	70	theme	arabinogalactan	744:758	arg1	FLAs					770:773	FLAs	770:773	FLAs	770:773	We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.
32359163	4	70	theme	arabinogalactan	744:758	arg1	proteins					760:767	the fasciclin-like arabinogalactan proteins	725:767	the fasciclin-like arabinogalactan proteins (FLAs)	725:774	We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.
32359163	1	71	theme	proteins	144:151	arg1	evolution					122:130	the evolution	118:130	the evolution of globular proteins	118:151	Approaches for studying the evolution of globular proteins are now well established yet are unsuitable for disordered sequences.
32359163	5	72	from	species	1071:1077	arg1	FLAs					1058:1061	2019 FLAs	1053:1061	2019 FLAs from 78 species	1053:1077	Sequence space projections of fasciclin domains from 2019 FLAs from 78 species identified distinct clusters corresponding to different types of fasciclin domains.
32359163	5	73	theme	fasciclin	1144:1152	arg1	domains					1154:1160	fasciclin domains	1144:1160	fasciclin domains	1144:1160	Sequence space projections of fasciclin domains from 2019 FLAs from 78 species identified distinct clusters corresponding to different types of fasciclin domains.
32359163	4	74	theme	-rich	932:936	arg1	AG					955:956	AG	955:956	AG	955:956	We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.
32359163	4	74	theme	-rich	932:936	arg1	arabinogalactan					938:952	proline (Pro)-rich arabinogalactan	919:952	proline (Pro)-rich arabinogalactan (AG) regions	919:965	We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.
32359163	8	75	theme	changing	1558:1565	arg1	architectures					1574:1586	the changing domain architectures	1554:1586	the changing domain architectures	1554:1586	We reconstruct the overall evolutionary history of the FLAs, annotated with the changing domain architectures, glycosylation motifs, number and length of AG regions, and disordered region sequence features.
32359163	2	76	theme	sequence-function	446:462	arg1	relationships					464:476	potential sequence-function relationships	436:476	potential sequence-function relationships	436:476	Our understanding of the evolution of proteins containing disordered regions therefore lags that of globular proteins, limiting our capacity to estimate their evolutionary history, classify paralogs, and identify potential sequence-function relationships.
32359163	0	77	theme	Disordered	30:39	arg1	Regions					41:47	Sequence-Diverse Disordered Regions	13:47	Sequence-Diverse Disordered Regions	13:47	Evolution of Sequence-Diverse Disordered Regions in a Protein Family: Order within the Chaos.
32359163	2	78	contain	containing	270:279	arg1	proteins					261:268	proteins	261:268	proteins containing disordered regions	261:298	Our understanding of the evolution of proteins containing disordered regions therefore lags that of globular proteins, limiting our capacity to estimate their evolutionary history, classify paralogs, and identify potential sequence-function relationships.
32359163	2	78	contain	containing	270:279	arg2	regions					292:298	disordered regions	281:298	disordered regions	281:298	Our understanding of the evolution of proteins containing disordered regions therefore lags that of globular proteins, limiting our capacity to estimate their evolutionary history, classify paralogs, and identify potential sequence-function relationships.
32359163	8	79	theme	glycosylation	1589:1601	arg1	motifs					1603:1608	glycosylation motifs	1589:1608	glycosylation motifs	1589:1608	We reconstruct the overall evolutionary history of the FLAs, annotated with the changing domain architectures, glycosylation motifs, number and length of AG regions, and disordered region sequence features.
32359163	5	80	theme	space	1009:1013	arg1	projections					1015:1025	Sequence space projections	1000:1025	Sequence space projections of fasciclin domains from 2019 FLAs from 78 species	1000:1077	Sequence space projections of fasciclin domains from 2019 FLAs from 78 species identified distinct clusters corresponding to different types of fasciclin domains.
32359163	7	81	theme	disordered	1311:1320	arg1	regions					1322:1328	disordered regions	1311:1328	disordered regions	1311:1328	Sequence features of the globular and disordered regions clearly correlate with one another, implying coevolution of these distinct regions, as well as with the N-linked and O-linked glycosylation motifs.
32359163	4	82	theme	Pro-depleted	978:989	arg1	regions					991:997	longer Pro-depleted regions	971:997	longer Pro-depleted regions	971:997	We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.
32359163	3	83	theme	disordered	656:665	arg1	regions					667:673	disordered regions	656:673	disordered regions	656:673	Here, we overcome these limitations by using new analytical approaches that project representations of sequence space to dissect the evolution of proteins with both ordered and disordered regions, and the correlated changes between these.
32359163	4	84	theme	variable	816:823	arg1	number					825:830	a variable number	814:830	a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions	814:997	We use the fasciclin-like arabinogalactan proteins (FLAs) as a model family, since they contain a variable number of globular fasciclin domains as well as several distinct types of disordered regions: proline (Pro)-rich arabinogalactan (AG) regions and longer Pro-depleted regions.
32359163	0	85	theme	Protein	54:60	arg1	Family					62:67	a Protein Family	52:67	a Protein Family	52:67	Evolution of Sequence-Diverse Disordered Regions in a Protein Family: Order within the Chaos.
32359163	7	86	theme	globular	1298:1305	arg1	features					1282:1289	Sequence features	1273:1289	Sequence features of the globular and disordered regions	1273:1328	Sequence features of the globular and disordered regions clearly correlate with one another, implying coevolution of these distinct regions, as well as with the N-linked and O-linked glycosylation motifs.
32359163	0	87	dep	Evolution	0:8	arg1	Order					70:74	Order	70:74	Evolution of Sequence-Diverse Disordered Regions in a Protein Family: Order within the Chaos.	0:92	Evolution of Sequence-Diverse Disordered Regions in a Protein Family: Order within the Chaos.
32359163	10	88	theme	life	1937:1940	arg1	kingdoms					1925:1932	all kingdoms	1921:1932	all kingdoms of life	1921:1940	These findings will inform research on the abundant disordered regions in protein families from all kingdoms of life.
32359163	2	89	theme	globular	323:330	arg1	proteins					332:339	globular proteins	323:339	globular proteins	323:339	Our understanding of the evolution of proteins containing disordered regions therefore lags that of globular proteins, limiting our capacity to estimate their evolutionary history, classify paralogs, and identify potential sequence-function relationships.
32359163	7	90	theme	Sequence	1273:1280	arg1	features					1282:1289	Sequence features	1273:1289	Sequence features of the globular and disordered regions	1273:1328	Sequence features of the globular and disordered regions clearly correlate with one another, implying coevolution of these distinct regions, as well as with the N-linked and O-linked glycosylation motifs.
32359163	8	91	theme	regions	1635:1641	arg1	length					1622:1627	length	1622:1627	length	1622:1627	We reconstruct the overall evolutionary history of the FLAs, annotated with the changing domain architectures, glycosylation motifs, number and length of AG regions, and disordered region sequence features.
32359163	8	91	theme	regions	1635:1641	arg1	number					1611:1616	number	1611:1616	number	1611:1616	We reconstruct the overall evolutionary history of the FLAs, annotated with the changing domain architectures, glycosylation motifs, number and length of AG regions, and disordered region sequence features.
32359163	8	91	theme	regions	1635:1641	arg1	architectures					1574:1586	the changing domain architectures	1554:1586	the changing domain architectures	1554:1586	We reconstruct the overall evolutionary history of the FLAs, annotated with the changing domain architectures, glycosylation motifs, number and length of AG regions, and disordered region sequence features.
32359163	8	91	theme	regions	1635:1641	arg1	motifs					1603:1608	glycosylation motifs	1589:1608	glycosylation motifs	1589:1608	We reconstruct the overall evolutionary history of the FLAs, annotated with the changing domain architectures, glycosylation motifs, number and length of AG regions, and disordered region sequence features.
32359163	8	91	theme	regions	1635:1641	arg1	features					1675:1682	disordered region sequence features	1648:1682	disordered region sequence features	1648:1682	We reconstruct the overall evolutionary history of the FLAs, annotated with the changing domain architectures, glycosylation motifs, number and length of AG regions, and disordered region sequence features.
34071405	0	0	theme	Human	86:90	arg1	Equivalents					97:107	Human Skin Equivalents	86:107	Human Skin Equivalents	86:107	Multitargeted Approach for the Optimization of Morphogenesis and Barrier Formation in Human Skin Equivalents.
34071405	7	1	theme	barrier	1088:1094	arg1	functionality					1096:1108	barrier functionality	1088:1108	barrier functionality	1088:1108	Importantly, barrier functionality was improved in the corresponding approach.
34071405	2	2	theme	human	318:322	arg1	skin					324:327	native human skin	311:327	native human skin in many fundamental aspects	311:355	Yet, three-dimensional (3D) human skin equivalents (HSEs) are able to mimic native human skin in many fundamental aspects.
34071405	8	3	from	functionality	1278:1290	arg1	FTM					1299:1301	the FTM	1295:1301	the FTM	1295:1301	In conclusion, our leading optimization approach substantially improved the epidermal morphogenesis, barrier formation, and functionality in the FTM, which therefore better resembled native human skin.
34071405	4	4	theme	barrier	527:533	arg1	formation					535:543	an improved lipid barrier formation	509:543	an improved lipid barrier formation	509:543	Recently, HSEs with an improved lipid barrier formation were generated by (i) incorporating chitosan in the dermal collagen matrix, (ii) reducing the external oxygen level to 3%, and (iii) inhibiting the liver X receptor (LXR).
34071405	2	5	theme	native	311:316	arg1	skin					324:327	native human skin	311:327	native human skin in many fundamental aspects	311:355	Yet, three-dimensional (3D) human skin equivalents (HSEs) are able to mimic native human skin in many fundamental aspects.
34071405	4	6	theme	lipid	521:525	arg1	formation					535:543	an improved lipid barrier formation	509:543	an improved lipid barrier formation	509:543	Recently, HSEs with an improved lipid barrier formation were generated by (i) incorporating chitosan in the dermal collagen matrix, (ii) reducing the external oxygen level to 3%, and (iii) inhibiting the liver X receptor (LXR).
34071405	4	7	dep	inhibiting	678:687	arg1	iii					673:675	iii	673:675	iii	673:675	Recently, HSEs with an improved lipid barrier formation were generated by (i) incorporating chitosan in the dermal collagen matrix, (ii) reducing the external oxygen level to 3%, and (iii) inhibiting the liver X receptor (LXR).
34071405	5	8	theme	triple-targeted	867:881	arg1	approaches					896:905	triple-targeted optimization approaches	867:905	triple-targeted optimization approaches	867:905	In this study, we aimed to determine the synergic effects in full-thickness models (FTMs) with combinations of these factors as single-, double-, and triple-targeted optimization approaches.
34071405	2	9	theme	skin	269:272	arg1	HSEs					287:290	HSEs	287:290	HSEs	287:290	Yet, three-dimensional (3D) human skin equivalents (HSEs) are able to mimic native human skin in many fundamental aspects.
34071405	2	9	theme	skin	269:272	arg1	equivalents					274:284	three-dimensional (3D) human skin equivalents	240:284	three-dimensional (3D) human skin equivalents (HSEs)	240:291	Yet, three-dimensional (3D) human skin equivalents (HSEs) are able to mimic native human skin in many fundamental aspects.
34071405	4	10	dep	reducing	626:633	arg1	ii					622:623	ii	622:623	ii	622:623	Recently, HSEs with an improved lipid barrier formation were generated by (i) incorporating chitosan in the dermal collagen matrix, (ii) reducing the external oxygen level to 3%, and (iii) inhibiting the liver X receptor (LXR).
34071405	0	11	theme	Skin	92:95	arg1	Equivalents					97:107	Human Skin Equivalents	86:107	Human Skin Equivalents	86:107	Multitargeted Approach for the Optimization of Morphogenesis and Barrier Formation in Human Skin Equivalents.
34071405	6	12	theme	better	1048:1053	arg1	organization					1061:1072	a better lipid organization	1046:1072	a better lipid organization	1046:1072	The collagen-chitosan FTM supplemented with the LXR inhibitor showed improved epidermal morphogenesis, an enhanced lipid composition, and a better lipid organization.
34071405	2	13	theme	human	263:267	arg1	HSEs					287:290	HSEs	287:290	HSEs	287:290	Yet, three-dimensional (3D) human skin equivalents (HSEs) are able to mimic native human skin in many fundamental aspects.
34071405	2	13	theme	human	263:267	arg1	equivalents					274:284	three-dimensional (3D) human skin equivalents	240:284	three-dimensional (3D) human skin equivalents (HSEs)	240:291	Yet, three-dimensional (3D) human skin equivalents (HSEs) are able to mimic native human skin in many fundamental aspects.
34071405	6	14	theme	epidermal	986:994	arg1	morphogenesis					996:1008	improved epidermal morphogenesis	977:1008	improved epidermal morphogenesis	977:1008	The collagen-chitosan FTM supplemented with the LXR inhibitor showed improved epidermal morphogenesis, an enhanced lipid composition, and a better lipid organization.
34071405	3	15	theme	lipid	381:385	arg1	formation					395:403	the epidermal lipid barrier formation	367:403	the epidermal lipid barrier formation	367:403	However, the epidermal lipid barrier formation, which is essential for the functionality of the skin barrier, remains compromised.
34071405	3	15	theme	lipid	381:385	arg1	essential					415:423	essential	415:423	essential	415:423	However, the epidermal lipid barrier formation, which is essential for the functionality of the skin barrier, remains compromised.
34071405	4	16	theme	dermal	597:602	arg1	matrix					613:618	the dermal collagen matrix	593:618	the dermal collagen matrix	593:618	Recently, HSEs with an improved lipid barrier formation were generated by (i) incorporating chitosan in the dermal collagen matrix, (ii) reducing the external oxygen level to 3%, and (iii) inhibiting the liver X receptor (LXR).
34071405	5	17	theme	synergic	758:765	arg1	effects					767:773	the synergic effects	754:773	the synergic effects in full-thickness models (FTMs) with combinations of these factors as single-, double-, and triple-targeted optimization approaches	754:905	In this study, we aimed to determine the synergic effects in full-thickness models (FTMs) with combinations of these factors as single-, double-, and triple-targeted optimization approaches.
34071405	3	18	theme	barrier	387:393	arg1	formation					395:403	the epidermal lipid barrier formation	367:403	the epidermal lipid barrier formation	367:403	However, the epidermal lipid barrier formation, which is essential for the functionality of the skin barrier, remains compromised.
34071405	3	18	theme	barrier	387:393	arg1	essential					415:423	essential	415:423	essential	415:423	However, the epidermal lipid barrier formation, which is essential for the functionality of the skin barrier, remains compromised.
34071405	4	19	with	HSEs	499:502	arg1	formation					535:543	an improved lipid barrier formation	509:543	an improved lipid barrier formation	509:543	Recently, HSEs with an improved lipid barrier formation were generated by (i) incorporating chitosan in the dermal collagen matrix, (ii) reducing the external oxygen level to 3%, and (iii) inhibiting the liver X receptor (LXR).
34071405	6	20	theme	LXR	956:958	arg1	inhibitor					960:968	the LXR inhibitor	952:968	the LXR inhibitor	952:968	The collagen-chitosan FTM supplemented with the LXR inhibitor showed improved epidermal morphogenesis, an enhanced lipid composition, and a better lipid organization.
34071405	1	21	theme	manifold	169:176	arg1	differences					178:188	the manifold differences	165:188	the manifold differences between the in vivo and in vitro conditions	165:232	In vitro skin tissue engineering is challenging due to the manifold differences between the in vivo and in vitro conditions.
34071405	0	22	from	Optimization	31:42	arg1	Equivalents					97:107	Human Skin Equivalents	86:107	Human Skin Equivalents	86:107	Multitargeted Approach for the Optimization of Morphogenesis and Barrier Formation in Human Skin Equivalents.
34071405	5	23	with	effects	767:773	arg1	combinations					812:823	combinations	812:823	combinations of these factors as single-, double-, and triple-targeted optimization approaches	812:905	In this study, we aimed to determine the synergic effects in full-thickness models (FTMs) with combinations of these factors as single-, double-, and triple-targeted optimization approaches.
34071405	2	24	theme	fundamental	337:347	arg1	aspects					349:355	many fundamental aspects	332:355	many fundamental aspects	332:355	Yet, three-dimensional (3D) human skin equivalents (HSEs) are able to mimic native human skin in many fundamental aspects.
34071405	5	25	from	effects	767:773	arg1	FTMs					801:804	FTMs	801:804	FTMs	801:804	In this study, we aimed to determine the synergic effects in full-thickness models (FTMs) with combinations of these factors as single-, double-, and triple-targeted optimization approaches.
34071405	5	25	from	effects	767:773	arg1	models					793:798	full-thickness models	778:798	full-thickness models (FTMs)	778:805	In this study, we aimed to determine the synergic effects in full-thickness models (FTMs) with combinations of these factors as single-, double-, and triple-targeted optimization approaches.
34071405	5	26	theme	full-thickness	778:791	arg1	FTMs					801:804	FTMs	801:804	FTMs	801:804	In this study, we aimed to determine the synergic effects in full-thickness models (FTMs) with combinations of these factors as single-, double-, and triple-targeted optimization approaches.
34071405	5	26	theme	full-thickness	778:791	arg1	models					793:798	full-thickness models	778:798	full-thickness models (FTMs)	778:805	In this study, we aimed to determine the synergic effects in full-thickness models (FTMs) with combinations of these factors as single-, double-, and triple-targeted optimization approaches.
34071405	2	27	theme	many	332:335	arg1	aspects					349:355	many fundamental aspects	332:355	many fundamental aspects	332:355	Yet, three-dimensional (3D) human skin equivalents (HSEs) are able to mimic native human skin in many fundamental aspects.
34071405	8	28	theme	native	1337:1342	arg1	skin					1350:1353	native human skin	1337:1353	native human skin	1337:1353	In conclusion, our leading optimization approach substantially improved the epidermal morphogenesis, barrier formation, and functionality in the FTM, which therefore better resembled native human skin.
34071405	2	29	from	skin	324:327	arg1	aspects					349:355	many fundamental aspects	332:355	many fundamental aspects	332:355	Yet, three-dimensional (3D) human skin equivalents (HSEs) are able to mimic native human skin in many fundamental aspects.
34071405	6	30	theme	lipid	1023:1027	arg1	composition					1029:1039	an enhanced lipid composition	1011:1039	an enhanced lipid composition	1011:1039	The collagen-chitosan FTM supplemented with the LXR inhibitor showed improved epidermal morphogenesis, an enhanced lipid composition, and a better lipid organization.
34071405	4	31	from	chitosan	581:588	arg1	matrix					613:618	the dermal collagen matrix	593:618	the dermal collagen matrix	593:618	Recently, HSEs with an improved lipid barrier formation were generated by (i) incorporating chitosan in the dermal collagen matrix, (ii) reducing the external oxygen level to 3%, and (iii) inhibiting the liver X receptor (LXR).
34071405	6	32	theme	collagen-chitosan	912:928	arg1	FTM					930:932	The collagen-chitosan FTM	908:932	The collagen-chitosan FTM supplemented with the LXR inhibitor	908:968	The collagen-chitosan FTM supplemented with the LXR inhibitor showed improved epidermal morphogenesis, an enhanced lipid composition, and a better lipid organization.
34071405	4	33	theme	improved	512:519	arg1	formation					535:543	an improved lipid barrier formation	509:543	an improved lipid barrier formation	509:543	Recently, HSEs with an improved lipid barrier formation were generated by (i) incorporating chitosan in the dermal collagen matrix, (ii) reducing the external oxygen level to 3%, and (iii) inhibiting the liver X receptor (LXR).
34071405	6	34	theme	lipid	1055:1059	arg1	organization					1061:1072	a better lipid organization	1046:1072	a better lipid organization	1046:1072	The collagen-chitosan FTM supplemented with the LXR inhibitor showed improved epidermal morphogenesis, an enhanced lipid composition, and a better lipid organization.
34071405	1	35	theme	In	110:111	arg1	engineering					131:141	In vitro skin tissue engineering	110:141	In vitro skin tissue engineering	110:141	In vitro skin tissue engineering is challenging due to the manifold differences between the in vivo and in vitro conditions.
34071405	1	36	theme	in	202:203	arg1	conditions					223:232	the in vivo and in vitro conditions	198:232	the in vivo and in vitro conditions	198:232	In vitro skin tissue engineering is challenging due to the manifold differences between the in vivo and in vitro conditions.
34071405	3	37	theme	skin	454:457	arg1	barrier					459:465	the skin barrier	450:465	the skin barrier	450:465	However, the epidermal lipid barrier formation, which is essential for the functionality of the skin barrier, remains compromised.
34071405	7	38	theme	corresponding	1130:1142	arg1	approach					1144:1151	the corresponding approach	1126:1151	the corresponding approach	1126:1151	Importantly, barrier functionality was improved in the corresponding approach.
34071405	2	39	dep	three-dimensional	240:256	arg1	3D					259:260	3D	259:260	3D	259:260	Yet, three-dimensional (3D) human skin equivalents (HSEs) are able to mimic native human skin in many fundamental aspects.
34071405	3	40	theme	epidermal	371:379	arg1	formation					395:403	the epidermal lipid barrier formation	367:403	the epidermal lipid barrier formation	367:403	However, the epidermal lipid barrier formation, which is essential for the functionality of the skin barrier, remains compromised.
34071405	3	40	theme	epidermal	371:379	arg1	essential					415:423	essential	415:423	essential	415:423	However, the epidermal lipid barrier formation, which is essential for the functionality of the skin barrier, remains compromised.
34071405	1	41	dep	in	214:215	arg1	vitro					217:221	vitro	217:221	vitro	217:221	In vitro skin tissue engineering is challenging due to the manifold differences between the in vivo and in vitro conditions.
34071405	4	42	theme	X	699:699	arg1	receptor					701:708	the liver X receptor	689:708	the liver X receptor (LXR)	689:714	Recently, HSEs with an improved lipid barrier formation were generated by (i) incorporating chitosan in the dermal collagen matrix, (ii) reducing the external oxygen level to 3%, and (iii) inhibiting the liver X receptor (LXR).
34071405	4	42	theme	X	699:699	arg1	LXR					711:713	LXR	711:713	LXR	711:713	Recently, HSEs with an improved lipid barrier formation were generated by (i) incorporating chitosan in the dermal collagen matrix, (ii) reducing the external oxygen level to 3%, and (iii) inhibiting the liver X receptor (LXR).
34071405	8	43	theme	optimization	1181:1192	arg1	approach					1194:1201	our leading optimization approach	1169:1201	our leading optimization approach	1169:1201	In conclusion, our leading optimization approach substantially improved the epidermal morphogenesis, barrier formation, and functionality in the FTM, which therefore better resembled native human skin.
34071405	4	44	dep	incorporating	567:579	arg1	i					564:564	i	564:564	i	564:564	Recently, HSEs with an improved lipid barrier formation were generated by (i) incorporating chitosan in the dermal collagen matrix, (ii) reducing the external oxygen level to 3%, and (iii) inhibiting the liver X receptor (LXR).
34071405	8	45	theme	leading	1173:1179	arg1	approach					1194:1201	our leading optimization approach	1169:1201	our leading optimization approach	1169:1201	In conclusion, our leading optimization approach substantially improved the epidermal morphogenesis, barrier formation, and functionality in the FTM, which therefore better resembled native human skin.
34071405	0	46	theme	Morphogenesis	47:59	arg1	Optimization					31:42	the Optimization	27:42	the Optimization of Morphogenesis and Barrier Formation in Human Skin Equivalents	27:107	Multitargeted Approach for the Optimization of Morphogenesis and Barrier Formation in Human Skin Equivalents.
34071405	5	47	theme	optimization	883:894	arg1	approaches					896:905	triple-targeted optimization approaches	867:905	triple-targeted optimization approaches	867:905	In this study, we aimed to determine the synergic effects in full-thickness models (FTMs) with combinations of these factors as single-, double-, and triple-targeted optimization approaches.
34071405	3	48	theme	barrier	459:465	arg1	functionality					433:445	the functionality	429:445	the functionality of the skin barrier	429:465	However, the epidermal lipid barrier formation, which is essential for the functionality of the skin barrier, remains compromised.
34071405	1	49	theme	skin	119:122	arg1	engineering					131:141	In vitro skin tissue engineering	110:141	In vitro skin tissue engineering	110:141	In vitro skin tissue engineering is challenging due to the manifold differences between the in vivo and in vitro conditions.
34071405	8	50	theme	human	1344:1348	arg1	skin					1350:1353	native human skin	1337:1353	native human skin	1337:1353	In conclusion, our leading optimization approach substantially improved the epidermal morphogenesis, barrier formation, and functionality in the FTM, which therefore better resembled native human skin.
34071405	4	51	theme	collagen	604:611	arg1	matrix					613:618	the dermal collagen matrix	593:618	the dermal collagen matrix	593:618	Recently, HSEs with an improved lipid barrier formation were generated by (i) incorporating chitosan in the dermal collagen matrix, (ii) reducing the external oxygen level to 3%, and (iii) inhibiting the liver X receptor (LXR).
34071405	1	52	theme	tissue	124:129	arg1	engineering					131:141	In vitro skin tissue engineering	110:141	In vitro skin tissue engineering	110:141	In vitro skin tissue engineering is challenging due to the manifold differences between the in vivo and in vitro conditions.
34071405	8	53	theme	barrier	1255:1261	arg1	formation					1263:1271	barrier formation	1255:1271	barrier formation	1255:1271	In conclusion, our leading optimization approach substantially improved the epidermal morphogenesis, barrier formation, and functionality in the FTM, which therefore better resembled native human skin.
34071405	1	54	theme	in	214:215	arg1	conditions					223:232	the in vivo and in vitro conditions	198:232	the in vivo and in vitro conditions	198:232	In vitro skin tissue engineering is challenging due to the manifold differences between the in vivo and in vitro conditions.
34071405	5	55	theme	factors	834:840	arg1	combinations					812:823	combinations	812:823	combinations of these factors as single-, double-, and triple-targeted optimization approaches	812:905	In this study, we aimed to determine the synergic effects in full-thickness models (FTMs) with combinations of these factors as single-, double-, and triple-targeted optimization approaches.
34071405	2	56	theme	three-dimensional	240:256	arg1	HSEs					287:290	HSEs	287:290	HSEs	287:290	Yet, three-dimensional (3D) human skin equivalents (HSEs) are able to mimic native human skin in many fundamental aspects.
34071405	2	56	theme	three-dimensional	240:256	arg1	equivalents					274:284	three-dimensional (3D) human skin equivalents	240:284	three-dimensional (3D) human skin equivalents (HSEs)	240:291	Yet, three-dimensional (3D) human skin equivalents (HSEs) are able to mimic native human skin in many fundamental aspects.
34071405	4	57	theme	external	639:646	arg1	level					655:659	the external oxygen level	635:659	the external oxygen level to 3%	635:665	Recently, HSEs with an improved lipid barrier formation were generated by (i) incorporating chitosan in the dermal collagen matrix, (ii) reducing the external oxygen level to 3%, and (iii) inhibiting the liver X receptor (LXR).
34071405	8	58	theme	epidermal	1230:1238	arg1	morphogenesis					1240:1252	epidermal morphogenesis	1230:1252	epidermal morphogenesis	1230:1252	In conclusion, our leading optimization approach substantially improved the epidermal morphogenesis, barrier formation, and functionality in the FTM, which therefore better resembled native human skin.
34071405	4	59	theme	oxygen	648:653	arg1	level					655:659	the external oxygen level	635:659	the external oxygen level to 3%	635:665	Recently, HSEs with an improved lipid barrier formation were generated by (i) incorporating chitosan in the dermal collagen matrix, (ii) reducing the external oxygen level to 3%, and (iii) inhibiting the liver X receptor (LXR).
34071405	0	60	theme	Formation	73:81	arg1	Optimization					31:42	the Optimization	27:42	the Optimization of Morphogenesis and Barrier Formation in Human Skin Equivalents	27:107	Multitargeted Approach for the Optimization of Morphogenesis and Barrier Formation in Human Skin Equivalents.
34071405	4	61	theme	liver	693:697	arg1	receptor					701:708	the liver X receptor	689:708	the liver X receptor (LXR)	689:714	Recently, HSEs with an improved lipid barrier formation were generated by (i) incorporating chitosan in the dermal collagen matrix, (ii) reducing the external oxygen level to 3%, and (iii) inhibiting the liver X receptor (LXR).
34071405	4	61	theme	liver	693:697	arg1	LXR					711:713	LXR	711:713	LXR	711:713	Recently, HSEs with an improved lipid barrier formation were generated by (i) incorporating chitosan in the dermal collagen matrix, (ii) reducing the external oxygen level to 3%, and (iii) inhibiting the liver X receptor (LXR).
34071405	6	62	theme	improved	977:984	arg1	morphogenesis					996:1008	improved epidermal morphogenesis	977:1008	improved epidermal morphogenesis	977:1008	The collagen-chitosan FTM supplemented with the LXR inhibitor showed improved epidermal morphogenesis, an enhanced lipid composition, and a better lipid organization.
34071405	8	63	from	morphogenesis	1240:1252	arg1	FTM					1299:1301	the FTM	1295:1301	the FTM	1295:1301	In conclusion, our leading optimization approach substantially improved the epidermal morphogenesis, barrier formation, and functionality in the FTM, which therefore better resembled native human skin.
34071405	0	64	theme	Barrier	65:71	arg1	Formation					73:81	Barrier Formation	65:81	Barrier Formation	65:81	Multitargeted Approach for the Optimization of Morphogenesis and Barrier Formation in Human Skin Equivalents.
34071405	6	65	theme	enhanced	1014:1021	arg1	composition					1029:1039	an enhanced lipid composition	1011:1039	an enhanced lipid composition	1011:1039	The collagen-chitosan FTM supplemented with the LXR inhibitor showed improved epidermal morphogenesis, an enhanced lipid composition, and a better lipid organization.
34071405	1	66	dep	in	202:203	arg1	vivo					205:208	vivo	205:208	vivo	205:208	In vitro skin tissue engineering is challenging due to the manifold differences between the in vivo and in vitro conditions.
34071405	1	67	dep	In	110:111	arg1	vitro					113:117	vitro	113:117	vitro	113:117	In vitro skin tissue engineering is challenging due to the manifold differences between the in vivo and in vitro conditions.
34071405	8	68	from	formation	1263:1271	arg1	FTM					1299:1301	the FTM	1295:1301	the FTM	1295:1301	In conclusion, our leading optimization approach substantially improved the epidermal morphogenesis, barrier formation, and functionality in the FTM, which therefore better resembled native human skin.
33676333	7	0	theme	study	1122:1126	arg1	findings					1105:1112	the findings	1101:1112	the findings of this study	1101:1126	According to the findings of this study, the application of alginate edible coatings containing TAEO, especially in Nano-form, can be very effective in controlling the growth of L. monocytogenes, as a foodborne pathogen, during storage; therefore, it is a good choice to be applied in the meat industry.
33676333	5	1	theme	current	755:761	arg1	study					763:767	the current study	751:767	the current study	751:767	Based on the obtained results of the current study, a comparison of different treatments with the blank samples (without any coating) showed that the highest considerable result was observed in the samples with Nano-emulsion coating (P < 0.05).
33676333	5	2	theme	blank	816:820	arg1	samples					822:828	the blank samples	812:828	the blank samples (without any coating)	812:850	Based on the obtained results of the current study, a comparison of different treatments with the blank samples (without any coating) showed that the highest considerable result was observed in the samples with Nano-emulsion coating (P < 0.05).
33676333	1	3	theme	essential	261:269	arg1	oil					271:273	Trachyspermum ammi essential oil	242:273	Trachyspermum ammi essential oil (TAEO)	242:280	The objective of this study was to determine the chemical composition and antibacterial activity of Trachyspermum ammi essential oil (TAEO).
33676333	1	3	theme	essential	261:269	arg1	TAEO					276:279	TAEO	276:279	TAEO	276:279	The objective of this study was to determine the chemical composition and antibacterial activity of Trachyspermum ammi essential oil (TAEO).
33676333	0	4	theme	edible	74:79	arg1	coating					81:87	alginate edible coating	65:87	alginate edible coating with Trachyspermum ammi essential oil nano-emulsion	65:139	Inhibition of Listeria monocytogenes growth in turkey fillets by alginate edible coating with Trachyspermum ammi essential oil nano-emulsion.
33676333	5	5	theme	treatments	796:805	arg1	comparison					772:781	a comparison	770:781	a comparison of different treatments with the blank samples (without any coating)	770:850	Based on the obtained results of the current study, a comparison of different treatments with the blank samples (without any coating) showed that the highest considerable result was observed in the samples with Nano-emulsion coating (P < 0.05).
33676333	0	6	with	coating	81:87	arg1	nano-emulsion					127:139	Trachyspermum ammi essential oil nano-emulsion	94:139	Trachyspermum ammi essential oil nano-emulsion	94:139	Inhibition of Listeria monocytogenes growth in turkey fillets by alginate edible coating with Trachyspermum ammi essential oil nano-emulsion.
33676333	1	7	theme	study	164:168	arg1	objective					146:154	The objective	142:154	The objective of this study	142:168	The objective of this study was to determine the chemical composition and antibacterial activity of Trachyspermum ammi essential oil (TAEO).
33676333	1	8	theme	oil	271:273	arg1	composition					200:210	chemical composition	191:210	chemical composition	191:210	The objective of this study was to determine the chemical composition and antibacterial activity of Trachyspermum ammi essential oil (TAEO).
33676333	1	8	theme	oil	271:273	arg1	activity					230:237	antibacterial activity	216:237	antibacterial activity	216:237	The objective of this study was to determine the chemical composition and antibacterial activity of Trachyspermum ammi essential oil (TAEO).
33676333	0	9	theme	Trachyspermum	94:106	arg1	nano-emulsion					127:139	Trachyspermum ammi essential oil nano-emulsion	94:139	Trachyspermum ammi essential oil nano-emulsion	94:139	Inhibition of Listeria monocytogenes growth in turkey fillets by alginate edible coating with Trachyspermum ammi essential oil nano-emulsion.
33676333	5	10	theme	highest	868:874	arg1	result					889:894	the highest considerable result	864:894	the highest considerable result	864:894	Based on the obtained results of the current study, a comparison of different treatments with the blank samples (without any coating) showed that the highest considerable result was observed in the samples with Nano-emulsion coating (P < 0.05).
33676333	2	11	theme	4 ± 1 °C	532:539	arg1	temperature					517:527	a temperature	515:527	a temperature of 4 ± 1 °C	515:539	Moreover, the present study comparatively investigated TAEO in the forms of emulsion and Nano-emulsion in alginate-based edible coatings against inoculated Listeria monocytogenes in turkey fillets during 12 days in cold storage (at a temperature of 4 ± 1 °C).
33676333	2	12	from	12 days	487:493	arg1	storage					503:509	cold storage	498:509	cold storage (at a temperature of 4 ± 1 °C)	498:540	Moreover, the present study comparatively investigated TAEO in the forms of emulsion and Nano-emulsion in alginate-based edible coatings against inoculated Listeria monocytogenes in turkey fillets during 12 days in cold storage (at a temperature of 4 ± 1 °C).
33676333	2	12	from	12 days	487:493	arg1	temperature					517:527	a temperature	515:527	a temperature of 4 ± 1 °C	515:539	Moreover, the present study comparatively investigated TAEO in the forms of emulsion and Nano-emulsion in alginate-based edible coatings against inoculated Listeria monocytogenes in turkey fillets during 12 days in cold storage (at a temperature of 4 ± 1 °C).
33676333	7	13	theme	alginate	1148:1155	arg1	coatings					1164:1171	alginate edible coatings	1148:1171	alginate edible coatings containing TAEO	1148:1187	According to the findings of this study, the application of alginate edible coatings containing TAEO, especially in Nano-form, can be very effective in controlling the growth of L. monocytogenes, as a foodborne pathogen, during storage; therefore, it is a good choice to be applied in the meat industry.
33676333	5	14	theme	considerable	876:887	arg1	result					889:894	the highest considerable result	864:894	the highest considerable result	864:894	Based on the obtained results of the current study, a comparison of different treatments with the blank samples (without any coating) showed that the highest considerable result was observed in the samples with Nano-emulsion coating (P < 0.05).
33676333	0	15	theme	essential	113:121	arg1	nano-emulsion					127:139	Trachyspermum ammi essential oil nano-emulsion	94:139	Trachyspermum ammi essential oil nano-emulsion	94:139	Inhibition of Listeria monocytogenes growth in turkey fillets by alginate edible coating with Trachyspermum ammi essential oil nano-emulsion.
33676333	7	16	contain	containing	1173:1182	arg2	TAEO					1184:1187	TAEO	1184:1187	TAEO	1184:1187	According to the findings of this study, the application of alginate edible coatings containing TAEO, especially in Nano-form, can be very effective in controlling the growth of L. monocytogenes, as a foodborne pathogen, during storage; therefore, it is a good choice to be applied in the meat industry.
33676333	7	16	contain	containing	1173:1182	arg1	coatings					1164:1171	alginate edible coatings	1148:1171	alginate edible coatings containing TAEO	1148:1187	According to the findings of this study, the application of alginate edible coatings containing TAEO, especially in Nano-form, can be very effective in controlling the growth of L. monocytogenes, as a foodborne pathogen, during storage; therefore, it is a good choice to be applied in the meat industry.
33676333	5	17	theme	study	763:767	arg1	results					740:746	the obtained results	727:746	the obtained results of the current study	727:767	Based on the obtained results of the current study, a comparison of different treatments with the blank samples (without any coating) showed that the highest considerable result was observed in the samples with Nano-emulsion coating (P < 0.05).
33676333	3	18	from	forms	617:621	arg1	levels					571:576	two levels	567:576	two levels of TAEO (in emulsion and Nano-emulsion forms)	567:622	Alginate solutions with two levels of TAEO (in emulsion and Nano-emulsion forms) were prepared in this study.
33676333	2	19	theme	edible	404:409	arg1	coatings					411:418	alginate-based edible coatings	389:418	alginate-based edible coatings	389:418	Moreover, the present study comparatively investigated TAEO in the forms of emulsion and Nano-emulsion in alginate-based edible coatings against inoculated Listeria monocytogenes in turkey fillets during 12 days in cold storage (at a temperature of 4 ± 1 °C).
33676333	0	20	theme	ammi	108:111	arg1	nano-emulsion					127:139	Trachyspermum ammi essential oil nano-emulsion	94:139	Trachyspermum ammi essential oil nano-emulsion	94:139	Inhibition of Listeria monocytogenes growth in turkey fillets by alginate edible coating with Trachyspermum ammi essential oil nano-emulsion.
33676333	3	21	theme	Alginate	543:550	arg1	solutions					552:560	Alginate solutions	543:560	Alginate solutions with two levels of TAEO (in emulsion and Nano-emulsion forms)	543:622	Alginate solutions with two levels of TAEO (in emulsion and Nano-emulsion forms) were prepared in this study.
33676333	2	22	theme	alginate-based	389:402	arg1	coatings					411:418	alginate-based edible coatings	389:418	alginate-based edible coatings	389:418	Moreover, the present study comparatively investigated TAEO in the forms of emulsion and Nano-emulsion in alginate-based edible coatings against inoculated Listeria monocytogenes in turkey fillets during 12 days in cold storage (at a temperature of 4 ± 1 °C).
33676333	5	23	theme	obtained	731:738	arg1	results					740:746	the obtained results	727:746	the obtained results of the current study	727:767	Based on the obtained results of the current study, a comparison of different treatments with the blank samples (without any coating) showed that the highest considerable result was observed in the samples with Nano-emulsion coating (P < 0.05).
33676333	0	24	from	growth	37:42	arg1	turkey					47:52	turkey	47:52	turkey	47:52	Inhibition of Listeria monocytogenes growth in turkey fillets by alginate edible coating with Trachyspermum ammi essential oil nano-emulsion.
33676333	2	25	from	forms	350:354	arg1	coatings					411:418	alginate-based edible coatings	389:418	alginate-based edible coatings	389:418	Moreover, the present study comparatively investigated TAEO in the forms of emulsion and Nano-emulsion in alginate-based edible coatings against inoculated Listeria monocytogenes in turkey fillets during 12 days in cold storage (at a temperature of 4 ± 1 °C).
33676333	6	26	theme	cold	1074:1077	arg1	storage					1079:1085	cold storage	1074:1085	cold storage	1074:1085	Nano-emulsion loaded alginate coating prevented the growth of listeria in turkey fillets even after 12 days of cold storage.
33676333	5	27	theme	different	786:794	arg1	treatments					796:805	different treatments	786:805	different treatments with the blank samples (without any coating)	786:850	Based on the obtained results of the current study, a comparison of different treatments with the blank samples (without any coating) showed that the highest considerable result was observed in the samples with Nano-emulsion coating (P < 0.05).
33676333	2	28	theme	turkey	465:470	arg1	fillets					472:478	turkey fillets	465:478	turkey fillets	465:478	Moreover, the present study comparatively investigated TAEO in the forms of emulsion and Nano-emulsion in alginate-based edible coatings against inoculated Listeria monocytogenes in turkey fillets during 12 days in cold storage (at a temperature of 4 ± 1 °C).
33676333	3	29	theme	TAEO	581:584	arg1	levels					571:576	two levels	567:576	two levels of TAEO (in emulsion and Nano-emulsion forms)	567:622	Alginate solutions with two levels of TAEO (in emulsion and Nano-emulsion forms) were prepared in this study.
33676333	2	30	theme	Nano-emulsion	372:384	arg1	forms					350:354	the forms	346:354	the forms of emulsion and Nano-emulsion in alginate-based edible coatings	346:418	Moreover, the present study comparatively investigated TAEO in the forms of emulsion and Nano-emulsion in alginate-based edible coatings against inoculated Listeria monocytogenes in turkey fillets during 12 days in cold storage (at a temperature of 4 ± 1 °C).
33676333	0	31	theme	monocytogenes	23:35	arg1	Inhibition					0:9	Inhibition	0:9	Inhibition of Listeria monocytogenes growth in turkey	0:52	Inhibition of Listeria monocytogenes growth in turkey fillets by alginate edible coating with Trachyspermum ammi essential oil nano-emulsion.
33676333	2	32	from	emulsion	359:366	arg1	coatings					411:418	alginate-based edible coatings	389:418	alginate-based edible coatings	389:418	Moreover, the present study comparatively investigated TAEO in the forms of emulsion and Nano-emulsion in alginate-based edible coatings against inoculated Listeria monocytogenes in turkey fillets during 12 days in cold storage (at a temperature of 4 ± 1 °C).
33676333	2	33	theme	present	297:303	arg1	study					305:309	the present study	293:309	the present study	293:309	Moreover, the present study comparatively investigated TAEO in the forms of emulsion and Nano-emulsion in alginate-based edible coatings against inoculated Listeria monocytogenes in turkey fillets during 12 days in cold storage (at a temperature of 4 ± 1 °C).
33676333	2	34	from	TAEO	338:341	arg1	forms					350:354	the forms	346:354	the forms of emulsion and Nano-emulsion in alginate-based edible coatings	346:418	Moreover, the present study comparatively investigated TAEO in the forms of emulsion and Nano-emulsion in alginate-based edible coatings against inoculated Listeria monocytogenes in turkey fillets during 12 days in cold storage (at a temperature of 4 ± 1 °C).
33676333	2	35	theme	Listeria	439:446	arg1	monocytogenes					448:460	inoculated Listeria monocytogenes	428:460	inoculated Listeria monocytogenes	428:460	Moreover, the present study comparatively investigated TAEO in the forms of emulsion and Nano-emulsion in alginate-based edible coatings against inoculated Listeria monocytogenes in turkey fillets during 12 days in cold storage (at a temperature of 4 ± 1 °C).
33676333	0	36	theme	Listeria	14:21	arg1	monocytogenes					23:35	Listeria monocytogenes	14:35	Listeria monocytogenes growth in turkey	14:52	Inhibition of Listeria monocytogenes growth in turkey fillets by alginate edible coating with Trachyspermum ammi essential oil nano-emulsion.
33676333	0	37	theme	oil	123:125	arg1	nano-emulsion					127:139	Trachyspermum ammi essential oil nano-emulsion	94:139	Trachyspermum ammi essential oil nano-emulsion	94:139	Inhibition of Listeria monocytogenes growth in turkey fillets by alginate edible coating with Trachyspermum ammi essential oil nano-emulsion.
33676333	7	38	theme	foodborne	1289:1297	arg1	pathogen					1299:1306	a foodborne pathogen	1287:1306	a foodborne pathogen	1287:1306	According to the findings of this study, the application of alginate edible coatings containing TAEO, especially in Nano-form, can be very effective in controlling the growth of L. monocytogenes, as a foodborne pathogen, during storage; therefore, it is a good choice to be applied in the meat industry.
33676333	2	39	theme	inoculated	428:437	arg1	monocytogenes					448:460	inoculated Listeria monocytogenes	428:460	inoculated Listeria monocytogenes	428:460	Moreover, the present study comparatively investigated TAEO in the forms of emulsion and Nano-emulsion in alginate-based edible coatings against inoculated Listeria monocytogenes in turkey fillets during 12 days in cold storage (at a temperature of 4 ± 1 °C).
33676333	2	40	theme	cold	498:501	arg1	storage					503:509	cold storage	498:509	cold storage (at a temperature of 4 ± 1 °C)	498:540	Moreover, the present study comparatively investigated TAEO in the forms of emulsion and Nano-emulsion in alginate-based edible coatings against inoculated Listeria monocytogenes in turkey fillets during 12 days in cold storage (at a temperature of 4 ± 1 °C).
33676333	7	41	theme	edible	1157:1162	arg1	coatings					1164:1171	alginate edible coatings	1148:1171	alginate edible coatings containing TAEO	1148:1187	According to the findings of this study, the application of alginate edible coatings containing TAEO, especially in Nano-form, can be very effective in controlling the growth of L. monocytogenes, as a foodborne pathogen, during storage; therefore, it is a good choice to be applied in the meat industry.
33676333	3	42	theme	emulsion	590:597	arg1	forms					617:621	emulsion and Nano-emulsion forms	590:621	emulsion and Nano-emulsion forms	590:621	Alginate solutions with two levels of TAEO (in emulsion and Nano-emulsion forms) were prepared in this study.
33676333	2	43	from	coatings	411:418	arg1	forms					350:354	the forms	346:354	the forms of emulsion and Nano-emulsion in alginate-based edible coatings	346:418	Moreover, the present study comparatively investigated TAEO in the forms of emulsion and Nano-emulsion in alginate-based edible coatings against inoculated Listeria monocytogenes in turkey fillets during 12 days in cold storage (at a temperature of 4 ± 1 °C).
33676333	6	44	theme	alginate	984:991	arg1	coating					993:999	Nano-emulsion loaded alginate coating	963:999	Nano-emulsion loaded alginate coating	963:999	Nano-emulsion loaded alginate coating prevented the growth of listeria in turkey fillets even after 12 days of cold storage.
33676333	7	45	theme	good	1344:1347	arg1	choice					1349:1354	a good choice	1342:1354	a good choice to be applied in the meat industry	1342:1389	According to the findings of this study, the application of alginate edible coatings containing TAEO, especially in Nano-form, can be very effective in controlling the growth of L. monocytogenes, as a foodborne pathogen, during storage; therefore, it is a good choice to be applied in the meat industry.
33676333	7	45	theme	good	1344:1347	arg1	it					1336:1337	it	1336:1337	it	1336:1337	According to the findings of this study, the application of alginate edible coatings containing TAEO, especially in Nano-form, can be very effective in controlling the growth of L. monocytogenes, as a foodborne pathogen, during storage; therefore, it is a good choice to be applied in the meat industry.
33676333	5	46	located	observed	900:907	arg1	samples					916:922	the samples	912:922	the samples with Nano-emulsion coating (P < 0.05)	912:960	Based on the obtained results of the current study, a comparison of different treatments with the blank samples (without any coating) showed that the highest considerable result was observed in the samples with Nano-emulsion coating (P < 0.05).
33676333	5	46	located	observed	900:907	arg2	result					889:894	the highest considerable result	864:894	the highest considerable result	864:894	Based on the obtained results of the current study, a comparison of different treatments with the blank samples (without any coating) showed that the highest considerable result was observed in the samples with Nano-emulsion coating (P < 0.05).
33676333	5	47	theme	Nano-emulsion	929:941	arg1	P < 0.05					952:959	P < 0.05	952:959	P < 0.05	952:959	Based on the obtained results of the current study, a comparison of different treatments with the blank samples (without any coating) showed that the highest considerable result was observed in the samples with Nano-emulsion coating (P < 0.05).
33676333	5	47	theme	Nano-emulsion	929:941	arg1	coating					943:949	Nano-emulsion coating	929:949	Nano-emulsion coating (P < 0.05)	929:960	Based on the obtained results of the current study, a comparison of different treatments with the blank samples (without any coating) showed that the highest considerable result was observed in the samples with Nano-emulsion coating (P < 0.05).
33676333	7	48	theme	monocytogenes	1269:1281	arg1	growth					1256:1261	the growth	1252:1261	the growth of L. monocytogenes	1252:1281	According to the findings of this study, the application of alginate edible coatings containing TAEO, especially in Nano-form, can be very effective in controlling the growth of L. monocytogenes, as a foodborne pathogen, during storage; therefore, it is a good choice to be applied in the meat industry.
33676333	1	49	theme	antibacterial	216:228	arg1	activity					230:237	antibacterial activity	216:237	antibacterial activity	216:237	The objective of this study was to determine the chemical composition and antibacterial activity of Trachyspermum ammi essential oil (TAEO).
33676333	3	50	with	solutions	552:560	arg1	levels					571:576	two levels	567:576	two levels of TAEO (in emulsion and Nano-emulsion forms)	567:622	Alginate solutions with two levels of TAEO (in emulsion and Nano-emulsion forms) were prepared in this study.
33676333	2	51	from	Nano-emulsion	372:384	arg1	coatings					411:418	alginate-based edible coatings	389:418	alginate-based edible coatings	389:418	Moreover, the present study comparatively investigated TAEO in the forms of emulsion and Nano-emulsion in alginate-based edible coatings against inoculated Listeria monocytogenes in turkey fillets during 12 days in cold storage (at a temperature of 4 ± 1 °C).
33676333	6	52	theme	loaded	977:982	arg1	coating					993:999	Nano-emulsion loaded alginate coating	963:999	Nano-emulsion loaded alginate coating	963:999	Nano-emulsion loaded alginate coating prevented the growth of listeria in turkey fillets even after 12 days of cold storage.
33676333	5	53	with	treatments	796:805	arg1	samples					822:828	the blank samples	812:828	the blank samples (without any coating)	812:850	Based on the obtained results of the current study, a comparison of different treatments with the blank samples (without any coating) showed that the highest considerable result was observed in the samples with Nano-emulsion coating (P < 0.05).
33676333	7	54	theme	meat	1377:1380	arg1	industry					1382:1389	the meat industry	1373:1389	the meat industry	1373:1389	According to the findings of this study, the application of alginate edible coatings containing TAEO, especially in Nano-form, can be very effective in controlling the growth of L. monocytogenes, as a foodborne pathogen, during storage; therefore, it is a good choice to be applied in the meat industry.
33676333	4	55	theme	bacterial	657:665	arg1	count					667:671	The bacterial count	653:671	The bacterial count	653:671	The bacterial count was performed on days 0, 1, 2, 4, 8, and 12.
33676333	2	56	from	temperature	517:527	arg1	12 days					487:493	12 days	487:493	12 days in cold storage (at a temperature of 4 ± 1 °C)	487:540	Moreover, the present study comparatively investigated TAEO in the forms of emulsion and Nano-emulsion in alginate-based edible coatings against inoculated Listeria monocytogenes in turkey fillets during 12 days in cold storage (at a temperature of 4 ± 1 °C).
33676333	2	56	from	temperature	517:527	arg1	storage					503:509	cold storage	498:509	cold storage (at a temperature of 4 ± 1 °C)	498:540	Moreover, the present study comparatively investigated TAEO in the forms of emulsion and Nano-emulsion in alginate-based edible coatings against inoculated Listeria monocytogenes in turkey fillets during 12 days in cold storage (at a temperature of 4 ± 1 °C).
33676333	0	57	dep	monocytogenes	23:35	arg1	growth					37:42	growth	37:42	Listeria monocytogenes growth in turkey	14:52	Inhibition of Listeria monocytogenes growth in turkey fillets by alginate edible coating with Trachyspermum ammi essential oil nano-emulsion.
33676333	6	58	theme	turkey	1037:1042	arg1	fillets					1044:1050	turkey fillets	1037:1050	turkey fillets	1037:1050	Nano-emulsion loaded alginate coating prevented the growth of listeria in turkey fillets even after 12 days of cold storage.
33676333	3	59	from	levels	571:576	arg1	forms					617:621	emulsion and Nano-emulsion forms	590:621	emulsion and Nano-emulsion forms	590:621	Alginate solutions with two levels of TAEO (in emulsion and Nano-emulsion forms) were prepared in this study.
33676333	6	60	theme	storage	1079:1085	arg1	12 days					1063:1069	12 days	1063:1069	12 days of cold storage	1063:1085	Nano-emulsion loaded alginate coating prevented the growth of listeria in turkey fillets even after 12 days of cold storage.
33676333	6	61	theme	Nano-emulsion	963:975	arg1	coating					993:999	Nano-emulsion loaded alginate coating	963:999	Nano-emulsion loaded alginate coating	963:999	Nano-emulsion loaded alginate coating prevented the growth of listeria in turkey fillets even after 12 days of cold storage.
33676333	6	62	from	growth	1015:1020	arg1	fillets					1044:1050	turkey fillets	1037:1050	turkey fillets	1037:1050	Nano-emulsion loaded alginate coating prevented the growth of listeria in turkey fillets even after 12 days of cold storage.
33676333	7	63	theme	coatings	1164:1171	arg1	effective					1227:1235	effective	1227:1235	effective	1227:1235	According to the findings of this study, the application of alginate edible coatings containing TAEO, especially in Nano-form, can be very effective in controlling the growth of L. monocytogenes, as a foodborne pathogen, during storage; therefore, it is a good choice to be applied in the meat industry.
33676333	7	63	theme	coatings	1164:1171	arg1	application					1133:1143	the application	1129:1143	the application	1129:1143	According to the findings of this study, the application of alginate edible coatings containing TAEO, especially in Nano-form, can be very effective in controlling the growth of L. monocytogenes, as a foodborne pathogen, during storage; therefore, it is a good choice to be applied in the meat industry.
33676333	0	64	theme	alginate	65:72	arg1	coating					81:87	alginate edible coating	65:87	alginate edible coating with Trachyspermum ammi essential oil nano-emulsion	65:139	Inhibition of Listeria monocytogenes growth in turkey fillets by alginate edible coating with Trachyspermum ammi essential oil nano-emulsion.
33676333	7	65	from	application	1133:1143	arg1	Nano-form					1204:1212	Nano-form	1204:1212	Nano-form	1204:1212	According to the findings of this study, the application of alginate edible coatings containing TAEO, especially in Nano-form, can be very effective in controlling the growth of L. monocytogenes, as a foodborne pathogen, during storage; therefore, it is a good choice to be applied in the meat industry.
33676333	3	66	theme	Nano-emulsion	603:615	arg1	forms					617:621	emulsion and Nano-emulsion forms	590:621	emulsion and Nano-emulsion forms	590:621	Alginate solutions with two levels of TAEO (in emulsion and Nano-emulsion forms) were prepared in this study.
33676333	1	67	theme	chemical	191:198	arg1	composition					200:210	chemical composition	191:210	chemical composition	191:210	The objective of this study was to determine the chemical composition and antibacterial activity of Trachyspermum ammi essential oil (TAEO).
33676333	6	68	theme	listeria	1025:1032	arg1	growth					1015:1020	the growth	1011:1020	the growth of listeria in turkey fillets	1011:1050	Nano-emulsion loaded alginate coating prevented the growth of listeria in turkey fillets even after 12 days of cold storage.
33676333	3	69	from	TAEO	581:584	arg1	forms					617:621	emulsion and Nano-emulsion forms	590:621	emulsion and Nano-emulsion forms	590:621	Alginate solutions with two levels of TAEO (in emulsion and Nano-emulsion forms) were prepared in this study.
33676333	1	70	theme	Trachyspermum	242:254	arg1	oil					271:273	Trachyspermum ammi essential oil	242:273	Trachyspermum ammi essential oil (TAEO)	242:280	The objective of this study was to determine the chemical composition and antibacterial activity of Trachyspermum ammi essential oil (TAEO).
33676333	1	70	theme	Trachyspermum	242:254	arg1	TAEO					276:279	TAEO	276:279	TAEO	276:279	The objective of this study was to determine the chemical composition and antibacterial activity of Trachyspermum ammi essential oil (TAEO).
33676333	5	71	with	samples	916:922	arg1	P < 0.05					952:959	P < 0.05	952:959	P < 0.05	952:959	Based on the obtained results of the current study, a comparison of different treatments with the blank samples (without any coating) showed that the highest considerable result was observed in the samples with Nano-emulsion coating (P < 0.05).
33676333	5	71	with	samples	916:922	arg1	coating					943:949	Nano-emulsion coating	929:949	Nano-emulsion coating (P < 0.05)	929:960	Based on the obtained results of the current study, a comparison of different treatments with the blank samples (without any coating) showed that the highest considerable result was observed in the samples with Nano-emulsion coating (P < 0.05).
33676333	1	72	dep	composition	200:210	arg1	the					187:189	the	187:189	the	187:189	The objective of this study was to determine the chemical composition and antibacterial activity of Trachyspermum ammi essential oil (TAEO).
33676333	2	73	theme	emulsion	359:366	arg1	forms					350:354	the forms	346:354	the forms of emulsion and Nano-emulsion in alginate-based edible coatings	346:418	Moreover, the present study comparatively investigated TAEO in the forms of emulsion and Nano-emulsion in alginate-based edible coatings against inoculated Listeria monocytogenes in turkey fillets during 12 days in cold storage (at a temperature of 4 ± 1 °C).
33676333	1	74	theme	ammi	256:259	arg1	oil					271:273	Trachyspermum ammi essential oil	242:273	Trachyspermum ammi essential oil (TAEO)	242:280	The objective of this study was to determine the chemical composition and antibacterial activity of Trachyspermum ammi essential oil (TAEO).
33676333	1	74	theme	ammi	256:259	arg1	TAEO					276:279	TAEO	276:279	TAEO	276:279	The objective of this study was to determine the chemical composition and antibacterial activity of Trachyspermum ammi essential oil (TAEO).
32762805	9	0	theme	predominant	876:886	arg1	MK11					906:909	MK11	906:909	MK11	906:909	The predominant menaquinones were MK11 and MK12.
32762805	9	0	theme	predominant	876:886	arg1	menaquinones					888:899	The predominant menaquinones	872:899	The predominant menaquinones	872:899	The predominant menaquinones were MK11 and MK12.
32762805	7	1	theme	nucleotide	631:640	arg1	identity					642:649	The average nucleotide identity	619:649	The average nucleotide identity	619:649	The average nucleotide identity and digital DNA-DNA hybridization values between strain NS18T and A. lahaulensis DSM 17612T based on their whole genomes were 85.1 and 28.7 %, respectively.
32762805	5	2	theme	sequence	505:512	arg1	similarity					514:523	98.2 % 16S rRNA gene sequence similarity	484:523	98.2 % 16S rRNA gene sequence similarity	484:523	Its closest phylogenetic neighbour was Agrococcus lahaulensis DSM 17612T with 98.2 % 16S rRNA gene sequence similarity.
32762805	13	3	theme	=NBRC	1257:1261	arg1	1K03759T					1276:1283	=NBRC 113859T=MCCC 1K03759T	1257:1283	=NBRC 113859T=MCCC 1K03759T	1257:1283	According to the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain NS18T (=NBRC 113859T=MCCC 1K03759T) represents a novel species, for which the name Agrococcus sediminis sp.
32762805	13	3	theme	=NBRC	1257:1261	arg1	NS18T					1250:1254	strain NS18T	1243:1254	strain NS18T (=NBRC 113859T=MCCC 1K03759T)	1243:1284	According to the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain NS18T (=NBRC 113859T=MCCC 1K03759T) represents a novel species, for which the name Agrococcus sediminis sp.
32762805	7	4	theme	average	623:629	arg1	identity					642:649	The average nucleotide identity	619:649	The average nucleotide identity	619:649	The average nucleotide identity and digital DNA-DNA hybridization values between strain NS18T and A. lahaulensis DSM 17612T based on their whole genomes were 85.1 and 28.7 %, respectively.
32762805	13	5	theme	phenotypic	1189:1198	arg1	analyses					1233:1240	phenotypic, chemotaxonomic and phylogenetic analyses	1189:1240	phenotypic, chemotaxonomic and phylogenetic analyses	1189:1240	According to the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain NS18T (=NBRC 113859T=MCCC 1K03759T) represents a novel species, for which the name Agrococcus sediminis sp.
32762805	3	6	theme	isolate	186:192	arg1	Cells					173:177	Cells	173:177	Cells of the isolate	173:192	Cells of the isolate were spherical, aerobic, non-motile, Gram-stain-positive and non-endospore-forming.
32762805	7	7	theme	hybridization	671:683	arg1	values					685:690	The average nucleotide identity and digital DNA-DNA hybridization values	619:690	The average nucleotide identity and digital DNA-DNA hybridization values between strain NS18T and A. lahaulensis DSM 17612T based on their whole genomes	619:770	The average nucleotide identity and digital DNA-DNA hybridization values between strain NS18T and A. lahaulensis DSM 17612T based on their whole genomes were 85.1 and 28.7 %, respectively.
32762805	7	8	theme	A.	717:718	arg1	17612T					736:741	A. lahaulensis DSM 17612T	717:741	A. lahaulensis DSM 17612T	717:741	The average nucleotide identity and digital DNA-DNA hybridization values between strain NS18T and A. lahaulensis DSM 17612T based on their whole genomes were 85.1 and 28.7 %, respectively.
32762805	13	9	dep	Agrococcus	1333:1342	arg1	sediminis					1344:1352	sediminis	1344:1352	sediminis	1344:1352	According to the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain NS18T (=NBRC 113859T=MCCC 1K03759T) represents a novel species, for which the name Agrococcus sediminis sp.
32762805	13	10	theme	Agrococcus	1333:1342	arg1	sp					1354:1355	the name Agrococcus sediminis sp	1324:1355	the name Agrococcus sediminis sp	1324:1355	According to the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain NS18T (=NBRC 113859T=MCCC 1K03759T) represents a novel species, for which the name Agrococcus sediminis sp.
32762805	6	11	theme	DNA	588:590	arg1	G+C content					592:602	its genomic DNA G+C content	576:602	its genomic DNA G+C content	576:602	The complete genome of NS18T was 2 736 037 bp and its genomic DNA G+C content was 72.8 mol%.
32762805	7	12	theme	strain	700:705	arg1	NS18T					707:711	strain NS18T	700:711	strain NS18T	700:711	The average nucleotide identity and digital DNA-DNA hybridization values between strain NS18T and A. lahaulensis DSM 17612T based on their whole genomes were 85.1 and 28.7 %, respectively.
32762805	11	13	theme	peptidoglycan	1044:1056	arg1	Ala					1063:1065	Ala	1063:1065	Ala	1063:1065	The components of the peptidoglycan were Ala, Gly, Asp, Thr and DAB.
32762805	11	13	theme	peptidoglycan	1044:1056	arg1	components					1026:1035	The components	1022:1035	The components of the peptidoglycan	1022:1056	The components of the peptidoglycan were Ala, Gly, Asp, Thr and DAB.
32762805	1	14	theme	lake	64:67	arg1	sediment					69:76	lake sediment	64:76	lake sediment	64:76	nov., an actinobacterium isolated from lake sediment.
32762805	4	15	theme	Phylogenetic	278:289	arg1	analysis					291:298	Phylogenetic analysis	278:298	Phylogenetic analysis based on 16S rRNA gene sequences	278:331	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NS18T clustered in a clade of the genus Agrococcus.
32762805	0	16	theme	sediminis	11:19	arg1	sp					21:22	Agrococcus sediminis sp	0:22	Agrococcus sediminis sp.	0:23	Agrococcus sediminis sp.
32762805	13	17	theme	phylogenetic	1220:1231	arg1	analyses					1233:1240	phenotypic, chemotaxonomic and phylogenetic analyses	1189:1240	phenotypic, chemotaxonomic and phylogenetic analyses	1189:1240	According to the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain NS18T (=NBRC 113859T=MCCC 1K03759T) represents a novel species, for which the name Agrococcus sediminis sp.
32762805	5	18	theme	%	489:489	arg1	similarity					514:523	98.2 % 16S rRNA gene sequence similarity	484:523	98.2 % 16S rRNA gene sequence similarity	484:523	Its closest phylogenetic neighbour was Agrococcus lahaulensis DSM 17612T with 98.2 % 16S rRNA gene sequence similarity.
32762805	0	19	theme	Agrococcus	0:9	arg1	sp					21:22	Agrococcus sediminis sp	0:22	Agrococcus sediminis sp.	0:23	Agrococcus sediminis sp.
32762805	13	20	theme	name	1328:1331	arg1	sp					1354:1355	the name Agrococcus sediminis sp	1324:1355	the name Agrococcus sediminis sp	1324:1355	According to the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain NS18T (=NBRC 113859T=MCCC 1K03759T) represents a novel species, for which the name Agrococcus sediminis sp.
32762805	7	21	theme	identity	642:649	arg1	values					685:690	The average nucleotide identity and digital DNA-DNA hybridization values	619:690	The average nucleotide identity and digital DNA-DNA hybridization values between strain NS18T and A. lahaulensis DSM 17612T based on their whole genomes	619:770	The average nucleotide identity and digital DNA-DNA hybridization values between strain NS18T and A. lahaulensis DSM 17612T based on their whole genomes were 85.1 and 28.7 %, respectively.
32762805	8	22	dep	anteiso-C17 	835:846	arg1	anteiso-C15 					855:866	anteiso-C15 	855:866	anteiso-C15 	855:866	The major fatty acids were anteiso-C17 : 0 and anteiso-C15 : 0.
32762805	8	22	dep	anteiso-C17 	835:846	arg1	 0					848:849	 0	848:849	 0	848:849	The major fatty acids were anteiso-C17 : 0 and anteiso-C15 : 0.
32762805	8	22	dep	anteiso-C17 	835:846	arg1	 0					868:869	 0	868:869	anteiso-C17 : 0 and anteiso-C15 : 0	835:869	The major fatty acids were anteiso-C17 : 0 and anteiso-C15 : 0.
32762805	13	23	theme	chemotaxonomic	1201:1214	arg1	analyses					1233:1240	phenotypic, chemotaxonomic and phylogenetic analyses	1189:1240	phenotypic, chemotaxonomic and phylogenetic analyses	1189:1240	According to the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain NS18T (=NBRC 113859T=MCCC 1K03759T) represents a novel species, for which the name Agrococcus sediminis sp.
32762805	10	24	theme	unidentified	1001:1012	arg1	lipids					1014:1019	two unidentified lipids	997:1019	two unidentified lipids	997:1019	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol and two unidentified lipids.
32762805	1	25	attach	isolated	50:57	arg2	actinobacterium					34:48	an actinobacterium	31:48	an actinobacterium isolated from lake sediment	31:76	nov., an actinobacterium isolated from lake sediment.
32762805	1	25	attach	isolated	50:57	arg1	sediment					69:76	lake sediment	64:76	lake sediment	64:76	nov., an actinobacterium isolated from lake sediment.
32762805	5	26	theme	Agrococcus	445:454	arg1	17612T					472:477	Agrococcus lahaulensis DSM 17612T	445:477	Agrococcus lahaulensis DSM 17612T	445:477	Its closest phylogenetic neighbour was Agrococcus lahaulensis DSM 17612T with 98.2 % 16S rRNA gene sequence similarity.
32762805	7	27	dep	A.	717:718	arg1	lahaulensis					720:730	lahaulensis	720:730	lahaulensis	720:730	The average nucleotide identity and digital DNA-DNA hybridization values between strain NS18T and A. lahaulensis DSM 17612T based on their whole genomes were 85.1 and 28.7 %, respectively.
32762805	12	28	contain	contained	1113:1121	arg2	glucose					1152:1158	glucose	1152:1158	glucose	1152:1158	The whole-cell sugars contained rhamnose, ribose, xylose and glucose.
32762805	12	28	contain	contained	1113:1121	arg2	xylose					1141:1146	xylose	1141:1146	xylose	1141:1146	The whole-cell sugars contained rhamnose, ribose, xylose and glucose.
32762805	12	28	contain	contained	1113:1121	arg2	ribose					1133:1138	ribose	1133:1138	ribose	1133:1138	The whole-cell sugars contained rhamnose, ribose, xylose and glucose.
32762805	12	28	contain	contained	1113:1121	arg2	rhamnose					1123:1130	rhamnose	1123:1130	rhamnose	1123:1130	The whole-cell sugars contained rhamnose, ribose, xylose and glucose.
32762805	12	28	contain	contained	1113:1121	arg1	sugars					1106:1111	The whole-cell sugars	1091:1111	The whole-cell sugars	1091:1111	The whole-cell sugars contained rhamnose, ribose, xylose and glucose.
32762805	2	29	theme	Lake	157:160	arg1	China					166:170	Taihu Lake, PR China	151:170	China	166:170	A novel strain, designated NS18T, was isolated from sediment sampled at Taihu Lake, PR China.
32762805	5	30	theme	lahaulensis	456:466	arg1	17612T					472:477	Agrococcus lahaulensis DSM 17612T	445:477	Agrococcus lahaulensis DSM 17612T	445:477	Its closest phylogenetic neighbour was Agrococcus lahaulensis DSM 17612T with 98.2 % 16S rRNA gene sequence similarity.
32762805	1	31	dep	actinobacterium	34:48	arg1	nov.					25:28	nov.	25:28	nov.	25:28	nov., an actinobacterium isolated from lake sediment.
32762805	7	32	theme	85.1	777:780	arg1	%					791:791	85.1 and 28.7 %	777:791	%	791:791	The average nucleotide identity and digital DNA-DNA hybridization values between strain NS18T and A. lahaulensis DSM 17612T based on their whole genomes were 85.1 and 28.7 %, respectively.
32762805	2	33	theme	Taihu	151:155	arg1	China					166:170	Taihu Lake, PR China	151:170	China	166:170	A novel strain, designated NS18T, was isolated from sediment sampled at Taihu Lake, PR China.
32762805	5	34	theme	DSM	468:470	arg1	17612T					472:477	Agrococcus lahaulensis DSM 17612T	445:477	Agrococcus lahaulensis DSM 17612T	445:477	Its closest phylogenetic neighbour was Agrococcus lahaulensis DSM 17612T with 98.2 % 16S rRNA gene sequence similarity.
32762805	6	35	theme	NS18T	549:553	arg1	genome					539:544	The complete genome	526:544	The complete genome of NS18T	526:553	The complete genome of NS18T was 2 736 037 bp and its genomic DNA G+C content was 72.8 mol%.
32762805	6	35	theme	NS18T	549:553	arg1	2 736 037 bp					559:570	2 736 037 bp	559:570	2 736 037 bp	559:570	The complete genome of NS18T was 2 736 037 bp and its genomic DNA G+C content was 72.8 mol%.
32762805	7	36	theme	whole	758:762	arg1	genomes					764:770	their whole genomes	752:770	their whole genomes	752:770	The average nucleotide identity and digital DNA-DNA hybridization values between strain NS18T and A. lahaulensis DSM 17612T based on their whole genomes were 85.1 and 28.7 %, respectively.
32762805	13	37	theme	113859T=MCCC	1263:1274	arg1	1K03759T					1276:1283	=NBRC 113859T=MCCC 1K03759T	1257:1283	=NBRC 113859T=MCCC 1K03759T	1257:1283	According to the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain NS18T (=NBRC 113859T=MCCC 1K03759T) represents a novel species, for which the name Agrococcus sediminis sp.
32762805	13	37	theme	113859T=MCCC	1263:1274	arg1	NS18T					1250:1254	strain NS18T	1243:1254	strain NS18T (=NBRC 113859T=MCCC 1K03759T)	1243:1284	According to the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain NS18T (=NBRC 113859T=MCCC 1K03759T) represents a novel species, for which the name Agrococcus sediminis sp.
32762805	7	38	theme	DNA-DNA	663:669	arg1	hybridization					671:683	digital DNA-DNA hybridization	655:683	digital DNA-DNA hybridization	655:683	The average nucleotide identity and digital DNA-DNA hybridization values between strain NS18T and A. lahaulensis DSM 17612T based on their whole genomes were 85.1 and 28.7 %, respectively.
32762805	10	39	theme	polar	925:929	arg1	lipids					931:936	The polar lipids	921:936	The polar lipids	921:936	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol and two unidentified lipids.
32762805	4	40	theme	gene	318:321	arg1	sequences					323:331	16S rRNA gene sequences	309:331	16S rRNA gene sequences	309:331	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NS18T clustered in a clade of the genus Agrococcus.
32762805	2	41	theme	novel	81:85	arg1	strain					87:92	A novel strain	79:92	A novel strain	79:92	A novel strain, designated NS18T, was isolated from sediment sampled at Taihu Lake, PR China.
32762805	7	42	theme	28.7 	786:790	arg1	%					791:791	85.1 and 28.7 %	777:791	%	791:791	The average nucleotide identity and digital DNA-DNA hybridization values between strain NS18T and A. lahaulensis DSM 17612T based on their whole genomes were 85.1 and 28.7 %, respectively.
32762805	4	43	theme	rRNA	313:316	arg1	sequences					323:331	16S rRNA gene sequences	309:331	16S rRNA gene sequences	309:331	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NS18T clustered in a clade of the genus Agrococcus.
32762805	12	44	theme	whole-cell	1095:1104	arg1	sugars					1106:1111	The whole-cell sugars	1091:1111	The whole-cell sugars	1091:1111	The whole-cell sugars contained rhamnose, ribose, xylose and glucose.
32762805	8	45	theme	fatty	818:822	arg1	anteiso-C17 					835:846	anteiso-C17 	835:846	anteiso-C17 	835:846	The major fatty acids were anteiso-C17 : 0 and anteiso-C15 : 0.
32762805	8	45	theme	fatty	818:822	arg1	acids					824:828	The major fatty acids	808:828	The major fatty acids	808:828	The major fatty acids were anteiso-C17 : 0 and anteiso-C15 : 0.
32762805	4	46	theme	genus	388:392	arg1	Agrococcus					394:403	the genus Agrococcus	384:403	the genus Agrococcus	384:403	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NS18T clustered in a clade of the genus Agrococcus.
32762805	7	47	theme	digital	655:661	arg1	hybridization					671:683	digital DNA-DNA hybridization	655:683	digital DNA-DNA hybridization	655:683	The average nucleotide identity and digital DNA-DNA hybridization values between strain NS18T and A. lahaulensis DSM 17612T based on their whole genomes were 85.1 and 28.7 %, respectively.
32762805	13	48	theme	analyses	1233:1240	arg1	results					1178:1184	the results	1174:1184	the results of phenotypic, chemotaxonomic and phylogenetic analyses	1174:1240	According to the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain NS18T (=NBRC 113859T=MCCC 1K03759T) represents a novel species, for which the name Agrococcus sediminis sp.
32762805	8	49	theme	major	812:816	arg1	anteiso-C17 					835:846	anteiso-C17 	835:846	anteiso-C17 	835:846	The major fatty acids were anteiso-C17 : 0 and anteiso-C15 : 0.
32762805	8	49	theme	major	812:816	arg1	acids					824:828	The major fatty acids	808:828	The major fatty acids	808:828	The major fatty acids were anteiso-C17 : 0 and anteiso-C15 : 0.
32762805	5	50	theme	gene	500:503	arg1	similarity					514:523	98.2 % 16S rRNA gene sequence similarity	484:523	98.2 % 16S rRNA gene sequence similarity	484:523	Its closest phylogenetic neighbour was Agrococcus lahaulensis DSM 17612T with 98.2 % 16S rRNA gene sequence similarity.
32762805	5	51	with	17612T	472:477	arg1	similarity					514:523	98.2 % 16S rRNA gene sequence similarity	484:523	98.2 % 16S rRNA gene sequence similarity	484:523	Its closest phylogenetic neighbour was Agrococcus lahaulensis DSM 17612T with 98.2 % 16S rRNA gene sequence similarity.
32762805	13	52	theme	strain	1243:1248	arg1	1K03759T					1276:1283	=NBRC 113859T=MCCC 1K03759T	1257:1283	=NBRC 113859T=MCCC 1K03759T	1257:1283	According to the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain NS18T (=NBRC 113859T=MCCC 1K03759T) represents a novel species, for which the name Agrococcus sediminis sp.
32762805	13	52	theme	strain	1243:1248	arg1	NS18T					1250:1254	strain NS18T	1243:1254	strain NS18T (=NBRC 113859T=MCCC 1K03759T)	1243:1284	According to the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain NS18T (=NBRC 113859T=MCCC 1K03759T) represents a novel species, for which the name Agrococcus sediminis sp.
32762805	5	53	theme	16S	491:493	arg1	similarity					514:523	98.2 % 16S rRNA gene sequence similarity	484:523	98.2 % 16S rRNA gene sequence similarity	484:523	Its closest phylogenetic neighbour was Agrococcus lahaulensis DSM 17612T with 98.2 % 16S rRNA gene sequence similarity.
32762805	2	54	attach	isolated	117:124	arg2	strain					87:92	A novel strain	79:92	A novel strain	79:92	A novel strain, designated NS18T, was isolated from sediment sampled at Taihu Lake, PR China.
32762805	2	54	attach	isolated	117:124	arg1	sediment					131:138	sediment	131:138	sediment sampled at Taihu Lake, PR China	131:170	A novel strain, designated NS18T, was isolated from sediment sampled at Taihu Lake, PR China.
32762805	5	55	theme	phylogenetic	418:429	arg1	neighbour					431:439	Its closest phylogenetic neighbour	406:439	Its closest phylogenetic neighbour	406:439	Its closest phylogenetic neighbour was Agrococcus lahaulensis DSM 17612T with 98.2 % 16S rRNA gene sequence similarity.
32762805	5	56	theme	98.2 	484:488	arg1	%					489:489	%	489:489	%	489:489	Its closest phylogenetic neighbour was Agrococcus lahaulensis DSM 17612T with 98.2 % 16S rRNA gene sequence similarity.
32762805	6	57	theme	complete	530:537	arg1	genome					539:544	The complete genome	526:544	The complete genome of NS18T	526:553	The complete genome of NS18T was 2 736 037 bp and its genomic DNA G+C content was 72.8 mol%.
32762805	6	57	theme	complete	530:537	arg1	2 736 037 bp					559:570	2 736 037 bp	559:570	2 736 037 bp	559:570	The complete genome of NS18T was 2 736 037 bp and its genomic DNA G+C content was 72.8 mol%.
32762805	4	58	theme	Agrococcus	394:403	arg1	clade					375:379	a clade	373:379	a clade of the genus Agrococcus	373:403	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NS18T clustered in a clade of the genus Agrococcus.
32762805	5	59	theme	rRNA	495:498	arg1	similarity					514:523	98.2 % 16S rRNA gene sequence similarity	484:523	98.2 % 16S rRNA gene sequence similarity	484:523	Its closest phylogenetic neighbour was Agrococcus lahaulensis DSM 17612T with 98.2 % 16S rRNA gene sequence similarity.
32762805	5	60	theme	closest	410:416	arg1	neighbour					431:439	Its closest phylogenetic neighbour	406:439	Its closest phylogenetic neighbour	406:439	Its closest phylogenetic neighbour was Agrococcus lahaulensis DSM 17612T with 98.2 % 16S rRNA gene sequence similarity.
32762805	13	61	theme	novel	1299:1303	arg1	species					1305:1311	a novel species	1297:1311	a novel species	1297:1311	According to the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain NS18T (=NBRC 113859T=MCCC 1K03759T) represents a novel species, for which the name Agrococcus sediminis sp.
32762805	7	62	theme	DSM	732:734	arg1	17612T					736:741	A. lahaulensis DSM 17612T	717:741	A. lahaulensis DSM 17612T	717:741	The average nucleotide identity and digital DNA-DNA hybridization values between strain NS18T and A. lahaulensis DSM 17612T based on their whole genomes were 85.1 and 28.7 %, respectively.
32762805	4	63	theme	16S	309:311	arg1	sequences					323:331	16S rRNA gene sequences	309:331	16S rRNA gene sequences	309:331	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NS18T clustered in a clade of the genus Agrococcus.
32762805	6	64	theme	genomic	580:586	arg1	G+C content					592:602	its genomic DNA G+C content	576:602	its genomic DNA G+C content	576:602	The complete genome of NS18T was 2 736 037 bp and its genomic DNA G+C content was 72.8 mol%.
32762805	6	65	theme	72.8 mol	608:615	arg1	%					616:616	72.8 mol%	608:616	72.8 mol%	608:616	The complete genome of NS18T was 2 736 037 bp and its genomic DNA G+C content was 72.8 mol%.
32762805	2	66	dep	China	166:170	arg1	PR					163:164	Taihu Lake, PR China	151:170	PR	163:164	A novel strain, designated NS18T, was isolated from sediment sampled at Taihu Lake, PR China.
33453253	5	0	theme	barrier	745:751	arg1	properties					753:762	barrier properties	745:762	barrier properties	745:762	The addition of RB led to improved film characteristics, including the thickness, mechanical properties, solubility, and water barrier properties.
33453253	5	1	theme	RB	634:635	arg1	addition					622:629	The addition	618:629	The addition of RB	618:635	The addition of RB led to improved film characteristics, including the thickness, mechanical properties, solubility, and water barrier properties.
33453253	9	2	theme	CS-RB	1319:1323	arg1	films					1335:1339	The CS-RB composite films	1315:1339	The CS-RB composite films	1315:1339	The CS-RB composite films showed potential as a novel environmentally friendly packaging material for shelf-life extension of refrigerated food products.
33453253	9	3	theme	shelf-life	1417:1426	arg1	extension					1428:1436	shelf-life extension	1417:1436	shelf-life extension of refrigerated food products	1417:1466	The CS-RB composite films showed potential as a novel environmentally friendly packaging material for shelf-life extension of refrigerated food products.
33453253	0	4	theme	food	99:102	arg1	packaging					104:112	antibacterial food packaging	85:112	antibacterial food packaging	85:112	Chitosan-riboflavin composite film based on photodynamic inactivation technology for antibacterial food packaging.
33453253	6	5	theme	Listeria	902:909	arg1	monocytogenes					911:923	Listeria monocytogenes	902:923	Listeria monocytogenes	902:923	The CS-RB5 composite films produced sufficient singlet oxygen under blue LED irradiation for 2 h to inactivate two food-borne pathogens (Listeria monocytogenes and Vibrio parahaemolyticus) and one spoilage bacteria (Shewanella baltica).
33453253	6	6	theme	singlet	812:818	arg1	oxygen					820:825	sufficient singlet oxygen	801:825	sufficient singlet oxygen	801:825	The CS-RB5 composite films produced sufficient singlet oxygen under blue LED irradiation for 2 h to inactivate two food-borne pathogens (Listeria monocytogenes and Vibrio parahaemolyticus) and one spoilage bacteria (Shewanella baltica).
33453253	3	7	theme	transparent	496:506	arg1	appearance					515:524	a visually appealing highly transparent yellow appearance	468:524	a visually appealing highly transparent yellow appearance	468:524	The CS-RB composite films exhibited good ultraviolet (UV)-barrier properties, and had a visually appealing highly transparent yellow appearance.
33453253	4	8	theme	even	572:575	arg1	dispersion					577:586	even dispersion	572:586	even dispersion of RB	572:592	Scanning electron microscopy (SEM) confirmed even dispersion of RB throughout the CS film.
33453253	7	9	theme	growth	1104:1109	arg1	inhibition					1080:1089	inhibition	1080:1089	inhibition of bacterial growth	1080:1109	The CS-RB composite films were assessed as a salmon packaging material, where inhibition of bacterial growth was observed.
33453253	3	10	theme	yellow	508:513	arg1	appearance					515:524	a visually appealing highly transparent yellow appearance	468:524	a visually appealing highly transparent yellow appearance	468:524	The CS-RB composite films exhibited good ultraviolet (UV)-barrier properties, and had a visually appealing highly transparent yellow appearance.
33453253	8	11	contain	has	1156:1158	arg2	potential					1164:1172	the potential to alleviate the issues associated with the excessive use of petrochemical materials, such as environmental pollution and limited resources	1160:1312	the potential to alleviate the issues associated with the excessive use of petrochemical materials, such as environmental pollution and limited resources	1160:1312	The film is biodegradable, and has the potential to alleviate the issues associated with the excessive use of petrochemical materials, such as environmental pollution and limited resources.
33453253	8	11	contain	has	1156:1158	arg1	biodegradable					1137:1149	biodegradable	1137:1149	biodegradable	1137:1149	The film is biodegradable, and has the potential to alleviate the issues associated with the excessive use of petrochemical materials, such as environmental pollution and limited resources.
33453253	8	11	contain	has	1156:1158	arg1	film					1129:1132	The film	1125:1132	The film	1125:1132	The film is biodegradable, and has the potential to alleviate the issues associated with the excessive use of petrochemical materials, such as environmental pollution and limited resources.
33453253	4	12	theme	RB	591:592	arg1	dispersion					577:586	even dispersion	572:586	even dispersion of RB	572:592	Scanning electron microscopy (SEM) confirmed even dispersion of RB throughout the CS film.
33453253	5	13	theme	improved	644:651	arg1	solubility					723:732	solubility	723:732	solubility	723:732	The addition of RB led to improved film characteristics, including the thickness, mechanical properties, solubility, and water barrier properties.
33453253	5	13	theme	improved	644:651	arg1	water					739:743	water	739:743	water	739:743	The addition of RB led to improved film characteristics, including the thickness, mechanical properties, solubility, and water barrier properties.
33453253	5	13	theme	improved	644:651	arg1	properties					711:720	mechanical properties	700:720	mechanical properties	700:720	The addition of RB led to improved film characteristics, including the thickness, mechanical properties, solubility, and water barrier properties.
33453253	5	13	theme	improved	644:651	arg1	characteristics					658:672	improved film characteristics	644:672	improved film characteristics	644:672	The addition of RB led to improved film characteristics, including the thickness, mechanical properties, solubility, and water barrier properties.
33453253	5	13	theme	improved	644:651	arg1	thickness					689:697	the thickness	685:697	the thickness	685:697	The addition of RB led to improved film characteristics, including the thickness, mechanical properties, solubility, and water barrier properties.
33453253	6	14	theme	Shewanella	981:990	arg1	2 h					858:860	2 h	858:860	2 h	858:860	The CS-RB5 composite films produced sufficient singlet oxygen under blue LED irradiation for 2 h to inactivate two food-borne pathogens (Listeria monocytogenes and Vibrio parahaemolyticus) and one spoilage bacteria (Shewanella baltica).
33453253	6	14	theme	Shewanella	981:990	arg1	baltica					992:998	Shewanella baltica	981:998	Shewanella baltica	981:998	The CS-RB5 composite films produced sufficient singlet oxygen under blue LED irradiation for 2 h to inactivate two food-borne pathogens (Listeria monocytogenes and Vibrio parahaemolyticus) and one spoilage bacteria (Shewanella baltica).
33453253	3	15	contain	had	464:466	arg2	appearance					515:524	a visually appealing highly transparent yellow appearance	468:524	a visually appealing highly transparent yellow appearance	468:524	The CS-RB composite films exhibited good ultraviolet (UV)-barrier properties, and had a visually appealing highly transparent yellow appearance.
33453253	3	15	contain	had	464:466	arg1	films					402:406	The CS-RB composite films	382:406	The CS-RB composite films	382:406	The CS-RB composite films exhibited good ultraviolet (UV)-barrier properties, and had a visually appealing highly transparent yellow appearance.
33453253	8	16	theme	materials	1249:1257	arg1	use					1228:1230	the excessive use	1214:1230	the excessive use of petrochemical materials	1214:1257	The film is biodegradable, and has the potential to alleviate the issues associated with the excessive use of petrochemical materials, such as environmental pollution and limited resources.
33453253	2	17	theme	chitosan	282:289	arg1	films					296:300	chitosan (CS) films	282:300	chitosan (CS) films containing photosensitizing riboflavin (RB)	282:344	This study focused on applying PDI to food packaging, where chitosan (CS) films containing photosensitizing riboflavin (RB) were prepared via solution casting.
33453253	9	18	theme	food	1454:1457	arg1	products					1459:1466	refrigerated food products	1441:1466	refrigerated food products	1441:1466	The CS-RB composite films showed potential as a novel environmentally friendly packaging material for shelf-life extension of refrigerated food products.
33453253	9	19	theme	refrigerated	1441:1452	arg1	products					1459:1466	refrigerated food products	1441:1466	refrigerated food products	1441:1466	The CS-RB composite films showed potential as a novel environmentally friendly packaging material for shelf-life extension of refrigerated food products.
33453253	7	20	theme	bacterial	1094:1102	arg1	growth					1104:1109	bacterial growth	1094:1109	bacterial growth	1094:1109	The CS-RB composite films were assessed as a salmon packaging material, where inhibition of bacterial growth was observed.
33453253	6	21	theme	composite	776:784	arg1	films					786:790	The CS-RB5 composite films	765:790	The CS-RB5 composite films	765:790	The CS-RB5 composite films produced sufficient singlet oxygen under blue LED irradiation for 2 h to inactivate two food-borne pathogens (Listeria monocytogenes and Vibrio parahaemolyticus) and one spoilage bacteria (Shewanella baltica).
33453253	0	22	theme	Chitosan-riboflavin	0:18	arg1	film					30:33	Chitosan-riboflavin composite film	0:33	Chitosan-riboflavin composite film	0:33	Chitosan-riboflavin composite film based on photodynamic inactivation technology for antibacterial food packaging.
33453253	8	23	theme	excessive	1218:1226	arg1	use					1228:1230	the excessive use	1214:1230	the excessive use of petrochemical materials	1214:1257	The film is biodegradable, and has the potential to alleviate the issues associated with the excessive use of petrochemical materials, such as environmental pollution and limited resources.
33453253	0	24	theme	composite	20:28	arg1	film					30:33	Chitosan-riboflavin composite film	0:33	Chitosan-riboflavin composite film	0:33	Chitosan-riboflavin composite film based on photodynamic inactivation technology for antibacterial food packaging.
33453253	5	25	dep	thickness	689:697	arg1	properties					753:762	barrier properties	745:762	barrier properties	745:762	The addition of RB led to improved film characteristics, including the thickness, mechanical properties, solubility, and water barrier properties.
33453253	6	26	theme	CS-RB5	769:774	arg1	films					786:790	The CS-RB5 composite films	765:790	The CS-RB5 composite films	765:790	The CS-RB5 composite films produced sufficient singlet oxygen under blue LED irradiation for 2 h to inactivate two food-borne pathogens (Listeria monocytogenes and Vibrio parahaemolyticus) and one spoilage bacteria (Shewanella baltica).
33453253	2	27	theme	photosensitizing	313:328	arg1	RB					342:343	RB	342:343	RB	342:343	This study focused on applying PDI to food packaging, where chitosan (CS) films containing photosensitizing riboflavin (RB) were prepared via solution casting.
33453253	2	27	theme	photosensitizing	313:328	arg1	riboflavin					330:339	photosensitizing riboflavin	313:339	photosensitizing riboflavin (RB)	313:344	This study focused on applying PDI to food packaging, where chitosan (CS) films containing photosensitizing riboflavin (RB) were prepared via solution casting.
33453253	6	28	theme	food-borne	880:889	arg1	pathogens					891:899	two food-borne pathogens	876:899	two food-borne pathogens (Listeria monocytogenes and Vibrio parahaemolyticus)	876:952	The CS-RB5 composite films produced sufficient singlet oxygen under blue LED irradiation for 2 h to inactivate two food-borne pathogens (Listeria monocytogenes and Vibrio parahaemolyticus) and one spoilage bacteria (Shewanella baltica).
33453253	8	29	theme	petrochemical	1235:1247	arg1	materials					1249:1257	petrochemical materials	1235:1257	petrochemical materials	1235:1257	The film is biodegradable, and has the potential to alleviate the issues associated with the excessive use of petrochemical materials, such as environmental pollution and limited resources.
33453253	3	30	theme	CS-RB	386:390	arg1	films					402:406	The CS-RB composite films	382:406	The CS-RB composite films	382:406	The CS-RB composite films exhibited good ultraviolet (UV)-barrier properties, and had a visually appealing highly transparent yellow appearance.
33453253	9	31	theme	composite	1325:1333	arg1	films					1335:1339	The CS-RB composite films	1315:1339	The CS-RB composite films	1315:1339	The CS-RB composite films showed potential as a novel environmentally friendly packaging material for shelf-life extension of refrigerated food products.
33453253	9	32	theme	novel	1363:1367	arg1	material					1404:1411	a novel environmentally friendly packaging material	1361:1411	a novel environmentally friendly packaging material for shelf-life extension of refrigerated food products	1361:1466	The CS-RB composite films showed potential as a novel environmentally friendly packaging material for shelf-life extension of refrigerated food products.
33453253	1	33	theme	Photodynamic	115:126	arg1	technology					172:181	a novel sterilization technology	150:181	a novel sterilization technology that has proven effective in medicine	150:219	Photodynamic inactivation (PDI) is a novel sterilization technology that has proven effective in medicine.
33453253	1	33	theme	Photodynamic	115:126	arg1	PDI					142:144	PDI	142:144	PDI	142:144	Photodynamic inactivation (PDI) is a novel sterilization technology that has proven effective in medicine.
33453253	1	33	theme	Photodynamic	115:126	arg1	inactivation					128:139	Photodynamic inactivation	115:139	Photodynamic inactivation (PDI)	115:145	Photodynamic inactivation (PDI) is a novel sterilization technology that has proven effective in medicine.
33453253	9	34	theme	environmentally	1369:1383	arg1	material					1404:1411	a novel environmentally friendly packaging material	1361:1411	a novel environmentally friendly packaging material for shelf-life extension of refrigerated food products	1361:1466	The CS-RB composite films showed potential as a novel environmentally friendly packaging material for shelf-life extension of refrigerated food products.
33453253	6	35	dep	2 h	858:860	arg1	bacteria					971:978	bacteria	971:978	bacteria	971:978	The CS-RB5 composite films produced sufficient singlet oxygen under blue LED irradiation for 2 h to inactivate two food-borne pathogens (Listeria monocytogenes and Vibrio parahaemolyticus) and one spoilage bacteria (Shewanella baltica).
33453253	1	36	theme	novel	152:156	arg1	inactivation					128:139	Photodynamic inactivation	115:139	Photodynamic inactivation (PDI)	115:145	Photodynamic inactivation (PDI) is a novel sterilization technology that has proven effective in medicine.
33453253	1	36	theme	novel	152:156	arg1	technology					172:181	a novel sterilization technology	150:181	a novel sterilization technology that has proven effective in medicine	150:219	Photodynamic inactivation (PDI) is a novel sterilization technology that has proven effective in medicine.
33453253	0	37	theme	photodynamic	44:55	arg1	technology					70:79	photodynamic inactivation technology	44:79	photodynamic inactivation technology for antibacterial food packaging	44:112	Chitosan-riboflavin composite film based on photodynamic inactivation technology for antibacterial food packaging.
33453253	8	38	theme	environmental	1268:1280	arg1	pollution					1282:1290	environmental pollution	1268:1290	environmental pollution	1268:1290	The film is biodegradable, and has the potential to alleviate the issues associated with the excessive use of petrochemical materials, such as environmental pollution and limited resources.
33453253	7	39	theme	salmon	1047:1052	arg1	material					1064:1071	a salmon packaging material	1045:1071	a salmon packaging material	1045:1071	The CS-RB composite films were assessed as a salmon packaging material, where inhibition of bacterial growth was observed.
33453253	7	39	theme	salmon	1047:1052	arg1	films					1022:1026	The CS-RB composite films	1002:1026	The CS-RB composite films	1002:1026	The CS-RB composite films were assessed as a salmon packaging material, where inhibition of bacterial growth was observed.
33453253	3	40	theme	-barrier	439:446	arg1	properties					448:457	good ultraviolet (UV)-barrier properties	418:457	good ultraviolet (UV)-barrier properties	418:457	The CS-RB composite films exhibited good ultraviolet (UV)-barrier properties, and had a visually appealing highly transparent yellow appearance.
33453253	3	41	theme	good	418:421	arg1	properties					448:457	good ultraviolet (UV)-barrier properties	418:457	good ultraviolet (UV)-barrier properties	418:457	The CS-RB composite films exhibited good ultraviolet (UV)-barrier properties, and had a visually appealing highly transparent yellow appearance.
33453253	4	42	theme	Scanning	527:534	arg1	SEM					557:559	SEM	557:559	SEM	557:559	Scanning electron microscopy (SEM) confirmed even dispersion of RB throughout the CS film.
33453253	4	42	theme	Scanning	527:534	arg1	microscopy					545:554	Scanning electron microscopy	527:554	Scanning electron microscopy (SEM)	527:560	Scanning electron microscopy (SEM) confirmed even dispersion of RB throughout the CS film.
33453253	5	43	theme	mechanical	700:709	arg1	properties					711:720	mechanical properties	700:720	mechanical properties	700:720	The addition of RB led to improved film characteristics, including the thickness, mechanical properties, solubility, and water barrier properties.
33453253	7	44	theme	composite	1012:1020	arg1	material					1064:1071	a salmon packaging material	1045:1071	a salmon packaging material	1045:1071	The CS-RB composite films were assessed as a salmon packaging material, where inhibition of bacterial growth was observed.
33453253	7	44	theme	composite	1012:1020	arg1	films					1022:1026	The CS-RB composite films	1002:1026	The CS-RB composite films	1002:1026	The CS-RB composite films were assessed as a salmon packaging material, where inhibition of bacterial growth was observed.
33453253	6	45	theme	Vibrio	929:934	arg1	parahaemolyticus					936:951	Vibrio parahaemolyticus	929:951	Vibrio parahaemolyticus	929:951	The CS-RB5 composite films produced sufficient singlet oxygen under blue LED irradiation for 2 h to inactivate two food-borne pathogens (Listeria monocytogenes and Vibrio parahaemolyticus) and one spoilage bacteria (Shewanella baltica).
33453253	3	46	theme	composite	392:400	arg1	films					402:406	The CS-RB composite films	382:406	The CS-RB composite films	382:406	The CS-RB composite films exhibited good ultraviolet (UV)-barrier properties, and had a visually appealing highly transparent yellow appearance.
33453253	5	47	theme	film	653:656	arg1	solubility					723:732	solubility	723:732	solubility	723:732	The addition of RB led to improved film characteristics, including the thickness, mechanical properties, solubility, and water barrier properties.
33453253	5	47	theme	film	653:656	arg1	water					739:743	water	739:743	water	739:743	The addition of RB led to improved film characteristics, including the thickness, mechanical properties, solubility, and water barrier properties.
33453253	5	47	theme	film	653:656	arg1	properties					711:720	mechanical properties	700:720	mechanical properties	700:720	The addition of RB led to improved film characteristics, including the thickness, mechanical properties, solubility, and water barrier properties.
33453253	5	47	theme	film	653:656	arg1	characteristics					658:672	improved film characteristics	644:672	improved film characteristics	644:672	The addition of RB led to improved film characteristics, including the thickness, mechanical properties, solubility, and water barrier properties.
33453253	5	47	theme	film	653:656	arg1	thickness					689:697	the thickness	685:697	the thickness	685:697	The addition of RB led to improved film characteristics, including the thickness, mechanical properties, solubility, and water barrier properties.
33453253	7	48	theme	packaging	1054:1062	arg1	material					1064:1071	a salmon packaging material	1045:1071	a salmon packaging material	1045:1071	The CS-RB composite films were assessed as a salmon packaging material, where inhibition of bacterial growth was observed.
33453253	7	48	theme	packaging	1054:1062	arg1	films					1022:1026	The CS-RB composite films	1002:1026	The CS-RB composite films	1002:1026	The CS-RB composite films were assessed as a salmon packaging material, where inhibition of bacterial growth was observed.
33453253	0	49	theme	inactivation	57:68	arg1	technology					70:79	photodynamic inactivation technology	44:79	photodynamic inactivation technology for antibacterial food packaging	44:112	Chitosan-riboflavin composite film based on photodynamic inactivation technology for antibacterial food packaging.
33453253	2	50	contain	containing	302:311	arg1	films					296:300	chitosan (CS) films	282:300	chitosan (CS) films containing photosensitizing riboflavin (RB)	282:344	This study focused on applying PDI to food packaging, where chitosan (CS) films containing photosensitizing riboflavin (RB) were prepared via solution casting.
33453253	2	50	contain	containing	302:311	arg2	RB					342:343	RB	342:343	RB	342:343	This study focused on applying PDI to food packaging, where chitosan (CS) films containing photosensitizing riboflavin (RB) were prepared via solution casting.
33453253	2	50	contain	containing	302:311	arg2	riboflavin					330:339	photosensitizing riboflavin	313:339	photosensitizing riboflavin (RB)	313:344	This study focused on applying PDI to food packaging, where chitosan (CS) films containing photosensitizing riboflavin (RB) were prepared via solution casting.
33453253	6	51	theme	LED	838:840	arg1	irradiation					842:852	blue LED irradiation	833:852	blue LED irradiation for 2 h to inactivate two food-borne pathogens (Listeria monocytogenes and Vibrio parahaemolyticus) and one spoilage bacteria (Shewanella baltica)	833:999	The CS-RB5 composite films produced sufficient singlet oxygen under blue LED irradiation for 2 h to inactivate two food-borne pathogens (Listeria monocytogenes and Vibrio parahaemolyticus) and one spoilage bacteria (Shewanella baltica).
33453253	1	52	from	medicine	212:219	arg1	effective					199:207	effective	199:207	effective	199:207	Photodynamic inactivation (PDI) is a novel sterilization technology that has proven effective in medicine.
33453253	3	53	theme	appealing	479:487	arg1	appearance					515:524	a visually appealing highly transparent yellow appearance	468:524	a visually appealing highly transparent yellow appearance	468:524	The CS-RB composite films exhibited good ultraviolet (UV)-barrier properties, and had a visually appealing highly transparent yellow appearance.
33453253	4	54	theme	electron	536:543	arg1	SEM					557:559	SEM	557:559	SEM	557:559	Scanning electron microscopy (SEM) confirmed even dispersion of RB throughout the CS film.
33453253	4	54	theme	electron	536:543	arg1	microscopy					545:554	Scanning electron microscopy	527:554	Scanning electron microscopy (SEM)	527:560	Scanning electron microscopy (SEM) confirmed even dispersion of RB throughout the CS film.
33453253	6	55	theme	blue	833:836	arg1	irradiation					842:852	blue LED irradiation	833:852	blue LED irradiation for 2 h to inactivate two food-borne pathogens (Listeria monocytogenes and Vibrio parahaemolyticus) and one spoilage bacteria (Shewanella baltica)	833:999	The CS-RB5 composite films produced sufficient singlet oxygen under blue LED irradiation for 2 h to inactivate two food-borne pathogens (Listeria monocytogenes and Vibrio parahaemolyticus) and one spoilage bacteria (Shewanella baltica).
33453253	6	56	theme	sufficient	801:810	arg1	oxygen					820:825	sufficient singlet oxygen	801:825	sufficient singlet oxygen	801:825	The CS-RB5 composite films produced sufficient singlet oxygen under blue LED irradiation for 2 h to inactivate two food-borne pathogens (Listeria monocytogenes and Vibrio parahaemolyticus) and one spoilage bacteria (Shewanella baltica).
33453253	8	57	theme	limited	1296:1302	arg1	resources					1304:1312	limited resources	1296:1312	limited resources	1296:1312	The film is biodegradable, and has the potential to alleviate the issues associated with the excessive use of petrochemical materials, such as environmental pollution and limited resources.
33453253	9	58	theme	friendly	1385:1392	arg1	material					1404:1411	a novel environmentally friendly packaging material	1361:1411	a novel environmentally friendly packaging material for shelf-life extension of refrigerated food products	1361:1466	The CS-RB composite films showed potential as a novel environmentally friendly packaging material for shelf-life extension of refrigerated food products.
33453253	7	59	theme	CS-RB	1006:1010	arg1	material					1064:1071	a salmon packaging material	1045:1071	a salmon packaging material	1045:1071	The CS-RB composite films were assessed as a salmon packaging material, where inhibition of bacterial growth was observed.
33453253	7	59	theme	CS-RB	1006:1010	arg1	films					1022:1026	The CS-RB composite films	1002:1026	The CS-RB composite films	1002:1026	The CS-RB composite films were assessed as a salmon packaging material, where inhibition of bacterial growth was observed.
33453253	1	60	from	effective	199:207	arg1	medicine					212:219	medicine	212:219	medicine	212:219	Photodynamic inactivation (PDI) is a novel sterilization technology that has proven effective in medicine.
33453253	9	61	theme	packaging	1394:1402	arg1	material					1404:1411	a novel environmentally friendly packaging material	1361:1411	a novel environmentally friendly packaging material for shelf-life extension of refrigerated food products	1361:1466	The CS-RB composite films showed potential as a novel environmentally friendly packaging material for shelf-life extension of refrigerated food products.
33453253	0	62	theme	antibacterial	85:97	arg1	packaging					104:112	antibacterial food packaging	85:112	antibacterial food packaging	85:112	Chitosan-riboflavin composite film based on photodynamic inactivation technology for antibacterial food packaging.
33453253	2	63	theme	food	260:263	arg1	packaging					265:273	food packaging	260:273	food packaging	260:273	This study focused on applying PDI to food packaging, where chitosan (CS) films containing photosensitizing riboflavin (RB) were prepared via solution casting.
33453253	2	64	theme	solution	364:371	arg1	casting					373:379	solution casting	364:379	solution casting	364:379	This study focused on applying PDI to food packaging, where chitosan (CS) films containing photosensitizing riboflavin (RB) were prepared via solution casting.
33453253	9	65	theme	products	1459:1466	arg1	extension					1428:1436	shelf-life extension	1417:1436	shelf-life extension of refrigerated food products	1417:1466	The CS-RB composite films showed potential as a novel environmentally friendly packaging material for shelf-life extension of refrigerated food products.
33453253	6	66	dep	pathogens	891:899	arg1	monocytogenes					911:923	Listeria monocytogenes	902:923	Listeria monocytogenes	902:923	The CS-RB5 composite films produced sufficient singlet oxygen under blue LED irradiation for 2 h to inactivate two food-borne pathogens (Listeria monocytogenes and Vibrio parahaemolyticus) and one spoilage bacteria (Shewanella baltica).
33453253	6	66	dep	pathogens	891:899	arg1	parahaemolyticus					936:951	Vibrio parahaemolyticus	929:951	Vibrio parahaemolyticus	929:951	The CS-RB5 composite films produced sufficient singlet oxygen under blue LED irradiation for 2 h to inactivate two food-borne pathogens (Listeria monocytogenes and Vibrio parahaemolyticus) and one spoilage bacteria (Shewanella baltica).
33453253	1	67	theme	sterilization	158:170	arg1	inactivation					128:139	Photodynamic inactivation	115:139	Photodynamic inactivation (PDI)	115:145	Photodynamic inactivation (PDI) is a novel sterilization technology that has proven effective in medicine.
33453253	1	67	theme	sterilization	158:170	arg1	technology					172:181	a novel sterilization technology	150:181	a novel sterilization technology that has proven effective in medicine	150:219	Photodynamic inactivation (PDI) is a novel sterilization technology that has proven effective in medicine.
33453253	4	68	theme	CS	609:610	arg1	film					612:615	the CS film	605:615	the CS film	605:615	Scanning electron microscopy (SEM) confirmed even dispersion of RB throughout the CS film.
34762581	11	0	theme	M.	1371:1372	arg1	strains					1336:1342	its reference strains	1322:1342	its reference strains	1322:1342	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	11	0	theme	M.	1371:1372	arg1	18904T					1390:1395	M. marinilacus DSM 18904T	1371:1395	M. marinilacus DSM 18904T	1371:1395	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	2	1	theme	novel	109:113	arg1	strain					165:170	A novel growth-promoting and indole acetic acid-producing strain	107:170	A novel growth-promoting and indole acetic acid-producing strain	107:170	A novel growth-promoting and indole acetic acid-producing strain, designated NEAU-LLBT, was isolated from cow dung collected from Shangzhi, Heilongjiang Province, PR China.
34762581	10	2	theme	strain	1209:1214	arg1	NEAU-LLBT					1216:1224	strain NEAU-LLBT	1209:1224	strain NEAU-LLBT	1209:1224	The genomic DNA G+C content of strain NEAU-LLBT was 70.2 mol%.
34762581	4	3	theme	16S	406:408	arg1	rRNA					410:413	16S rRNA	406:413	16S rRNA gene sequence	406:427	Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain NEAU-LLBT belonged to the genus Microbacterium.
34762581	4	4	theme	gene	415:418	arg1	sequence					420:427	16S rRNA gene sequence	406:427	16S rRNA gene sequence	406:427	Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain NEAU-LLBT belonged to the genus Microbacterium.
34762581	2	5	theme	acetic	143:148	arg1	strain					165:170	A novel growth-promoting and indole acetic acid-producing strain	107:170	A novel growth-promoting and indole acetic acid-producing strain	107:170	A novel growth-promoting and indole acetic acid-producing strain, designated NEAU-LLBT, was isolated from cow dung collected from Shangzhi, Heilongjiang Province, PR China.
34762581	11	6	theme	hybridization	1510:1522	arg1	level					1485:1489	the level	1481:1489	the level of digital DNA-DNA hybridization between them	1481:1535	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	11	6	theme	hybridization	1510:1522	arg1	%					1562:1562	23.8, 22.6 and 21.8 %	1542:1562	%	1562:1562	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	2	7	theme	PR	270:271	arg1	Province					260:267	Shangzhi, Heilongjiang Province	237:267	Province	260:267	A novel growth-promoting and indole acetic acid-producing strain, designated NEAU-LLBT, was isolated from cow dung collected from Shangzhi, Heilongjiang Province, PR China.
34762581	2	7	theme	PR	270:271	arg1	China					273:277	PR China	270:277	PR China	270:277	A novel growth-promoting and indole acetic acid-producing strain, designated NEAU-LLBT, was isolated from cow dung collected from Shangzhi, Heilongjiang Province, PR China.
34762581	5	8	theme	16S	525:527	arg1	similarities					543:554	high 16S rRNA sequence similarities	520:554	high 16S rRNA sequence similarities of 98.81 and 98.41 % to Microbacterium paludicola DSM 16915T and Microbacterium marinilacus DSM 18904T, and less than 98 % to other members of the genus Microbacterium	520:722	Strain NEAU-LLBT had high 16S rRNA sequence similarities of 98.81 and 98.41 % to Microbacterium paludicola DSM 16915T and Microbacterium marinilacus DSM 18904T, and less than 98 % to other members of the genus Microbacterium.
34762581	4	9	theme	Phylogenetic	375:386	arg1	analysis					388:395	Phylogenetic analysis	375:395	Phylogenetic analysis based on 16S rRNA gene sequence	375:427	Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain NEAU-LLBT belonged to the genus Microbacterium.
34762581	11	10	theme	digital	1494:1500	arg1	hybridization					1510:1522	digital DNA-DNA hybridization	1494:1522	digital DNA-DNA hybridization between them	1494:1535	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	7	11	contain	contained	849:857	arg2	ornithine					898:906	ornithine	898:906	ornithine	898:906	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	11	contain	contained	849:857	arg2	acid					883:886	aspartic acid	874:886	aspartic acid	874:886	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	11	contain	contained	849:857	arg2	glycine					889:895	glycine	889:895	glycine	889:895	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	11	contain	contained	849:857	arg2	amount					920:925	a small amount	912:925	a small amount of alanine	912:936	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	11	contain	contained	849:857	arg2	alanine					930:936	alanine	930:936	alanine	930:936	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	11	contain	contained	849:857	arg1	peptidoglycan					835:847	The peptidoglycan	831:847	The peptidoglycan	831:847	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	11	contain	contained	849:857	arg2	acid					868:871	glutamic acid	859:871	glutamic acid	859:871	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	11	contain	contained	849:857	arg2	acid					980:983	the diagnostic diamino acid	957:983	the diagnostic diamino acid	957:983	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	8	12	theme	major	990:994	arg1	diphosphatidylglycerol					1014:1035	diphosphatidylglycerol	1014:1035	diphosphatidylglycerol	1014:1035	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid.
34762581	8	12	theme	major	990:994	arg1	lipids					1002:1007	The major polar lipids	986:1007	The major polar lipids	986:1007	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid.
34762581	11	13	theme	reference	1326:1334	arg1	strains					1336:1342	its reference strains	1322:1342	its reference strains	1322:1342	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	11	13	theme	reference	1326:1334	arg1	D8007T					1415:1420	D8007T	1415:1420	D8007T	1415:1420	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	11	13	theme	reference	1326:1334	arg1	18904T					1390:1395	M. marinilacus DSM 18904T	1371:1395	M. marinilacus DSM 18904T	1371:1395	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	11	13	theme	reference	1326:1334	arg1	16915T					1363:1368	M. paludicola DSM 16915T	1345:1368	M. paludicola DSM 16915T	1345:1368	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	1	14	attach	isolated	83:90	arg1	cow					97:99	cow dung	97:104	cow dung	97:104	nov., an indole acetic acid-producing actinobacterium isolated from cow dung.
34762581	1	14	attach	isolated	83:90	arg2	actinobacterium					67:81	an indole acetic acid-producing actinobacterium	35:81	an indole acetic acid-producing actinobacterium isolated from cow dung	35:104	nov., an indole acetic acid-producing actinobacterium isolated from cow dung.
34762581	5	15	theme	Microbacterium	709:722	arg1	members					688:694	other members	682:694	other members of the genus Microbacterium	682:722	Strain NEAU-LLBT had high 16S rRNA sequence similarities of 98.81 and 98.41 % to Microbacterium paludicola DSM 16915T and Microbacterium marinilacus DSM 18904T, and less than 98 % to other members of the genus Microbacterium.
34762581	5	16	theme	Strain	499:504	arg1	NEAU-LLBT					506:514	Strain NEAU-LLBT	499:514	Strain NEAU-LLBT	499:514	Strain NEAU-LLBT had high 16S rRNA sequence similarities of 98.81 and 98.41 % to Microbacterium paludicola DSM 16915T and Microbacterium marinilacus DSM 18904T, and less than 98 % to other members of the genus Microbacterium.
34762581	3	17	theme	NEAU-LLBT	296:304	arg1	Cells					280:284	Cells	280:284	Cells of strain NEAU-LLBT	280:304	Cells of strain NEAU-LLBT were Gram-stain-positive, non-motile, aerobic and non-spore-forming.
34762581	1	18	dep	actinobacterium	67:81	arg1	nov.					29:32	nov.	29:32	nov.	29:32	nov., an indole acetic acid-producing actinobacterium isolated from cow dung.
34762581	11	19	theme	paludicola	1348:1357	arg1	strains					1336:1342	its reference strains	1322:1342	its reference strains	1322:1342	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	11	19	theme	paludicola	1348:1357	arg1	16915T					1363:1368	M. paludicola DSM 16915T	1345:1368	M. paludicola DSM 16915T	1345:1368	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	2	20	theme	dung	217:220	arg1	cow					213:215	cow dung	213:220	cow dung collected from Shangzhi, Heilongjiang Province, PR China	213:277	A novel growth-promoting and indole acetic acid-producing strain, designated NEAU-LLBT, was isolated from cow dung collected from Shangzhi, Heilongjiang Province, PR China.
34762581	5	21	theme	%	575:575	arg1	similarities					543:554	high 16S rRNA sequence similarities	520:554	high 16S rRNA sequence similarities of 98.81 and 98.41 % to Microbacterium paludicola DSM 16915T and Microbacterium marinilacus DSM 18904T, and less than 98 % to other members of the genus Microbacterium	520:722	Strain NEAU-LLBT had high 16S rRNA sequence similarities of 98.81 and 98.41 % to Microbacterium paludicola DSM 16915T and Microbacterium marinilacus DSM 18904T, and less than 98 % to other members of the genus Microbacterium.
34762581	12	22	theme	strain	1637:1642	arg1	NEAU-LLBT					1644:1652	strain NEAU-LLBT	1637:1652	strain NEAU-LLBT	1637:1652	Based on the phenotypic, phylogenetic and genotypic data, strain NEAU-LLBT is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium stercoris sp.
34762581	12	23	theme	novel	1683:1687	arg1	species					1689:1695	a novel species	1681:1695	a novel species	1681:1695	Based on the phenotypic, phylogenetic and genotypic data, strain NEAU-LLBT is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium stercoris sp.
34762581	5	24	theme	sequence	534:541	arg1	similarities					543:554	high 16S rRNA sequence similarities	520:554	high 16S rRNA sequence similarities of 98.81 and 98.41 % to Microbacterium paludicola DSM 16915T and Microbacterium marinilacus DSM 18904T, and less than 98 % to other members of the genus Microbacterium	520:722	Strain NEAU-LLBT had high 16S rRNA sequence similarities of 98.81 and 98.41 % to Microbacterium paludicola DSM 16915T and Microbacterium marinilacus DSM 18904T, and less than 98 % to other members of the genus Microbacterium.
34762581	4	25	theme	strain	444:449	arg1	NEAU-LLBT					451:459	strain NEAU-LLBT	444:459	strain NEAU-LLBT	444:459	Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain NEAU-LLBT belonged to the genus Microbacterium.
34762581	11	26	theme	identity	1277:1284	arg1	values					1286:1291	the average nucleotide identity values	1254:1291	the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T,	1254:1421	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	11	26	theme	identity	1277:1284	arg1	%					1460:1460	81.1, 79.4 and 78.7 %	1440:1460	81.1, 79.4 and 78.7 %	1440:1460	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	11	27	theme	average	1258:1264	arg1	values					1286:1291	the average nucleotide identity values	1254:1291	the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T,	1254:1421	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	11	27	theme	average	1258:1264	arg1	%					1460:1460	81.1, 79.4 and 78.7 %	1440:1460	81.1, 79.4 and 78.7 %	1440:1460	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	9	28	theme	fatty	1101:1105	arg1	acids					1107:1111	The major fatty acids	1091:1111	The major fatty acids	1091:1111	The major fatty acids were identified as anteiso-C15 : 0, iso-C16 : 0 and iso-C17 : 0.
34762581	9	28	theme	fatty	1101:1105	arg1	anteiso-C15 					1132:1143	anteiso-C15 	1132:1143	anteiso-C15 	1132:1143	The major fatty acids were identified as anteiso-C15 : 0, iso-C16 : 0 and iso-C17 : 0.
34762581	8	29	theme	unidentified	1066:1077	arg1	glycolipid					1079:1088	an unidentified glycolipid	1063:1088	an unidentified glycolipid	1063:1088	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid.
34762581	7	30	theme	diagnostic	961:970	arg1	alanine					930:936	alanine	930:936	alanine	930:936	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	30	theme	diagnostic	961:970	arg1	acid					868:871	glutamic acid	859:871	glutamic acid	859:871	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	30	theme	diagnostic	961:970	arg1	ornithine					898:906	ornithine	898:906	ornithine	898:906	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	30	theme	diagnostic	961:970	arg1	acid					980:983	the diagnostic diamino acid	957:983	the diagnostic diamino acid	957:983	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	30	theme	diagnostic	961:970	arg1	acid					883:886	aspartic acid	874:886	aspartic acid	874:886	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	30	theme	diagnostic	961:970	arg1	glycine					889:895	glycine	889:895	glycine	889:895	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	30	theme	diagnostic	961:970	arg1	amount					920:925	a small amount	912:925	a small amount of alanine	912:936	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	1	31	theme	acid-producing	52:65	arg1	actinobacterium					67:81	an indole acetic acid-producing actinobacterium	35:81	an indole acetic acid-producing actinobacterium isolated from cow dung	35:104	nov., an indole acetic acid-producing actinobacterium isolated from cow dung.
34762581	5	32	theme	Microbacterium	580:593	arg1	16915T					610:615	Microbacterium paludicola DSM 16915T	580:615	Microbacterium paludicola DSM 16915T	580:615	Strain NEAU-LLBT had high 16S rRNA sequence similarities of 98.81 and 98.41 % to Microbacterium paludicola DSM 16915T and Microbacterium marinilacus DSM 18904T, and less than 98 % to other members of the genus Microbacterium.
34762581	0	33	theme	Microbacterium	0:13	arg1	sp					25:26	Microbacterium stercoris sp	0:26	Microbacterium stercoris sp.	0:27	Microbacterium stercoris sp.
34762581	5	34	theme	DSM	606:608	arg1	16915T					610:615	Microbacterium paludicola DSM 16915T	580:615	Microbacterium paludicola DSM 16915T	580:615	Strain NEAU-LLBT had high 16S rRNA sequence similarities of 98.81 and 98.41 % to Microbacterium paludicola DSM 16915T and Microbacterium marinilacus DSM 18904T, and less than 98 % to other members of the genus Microbacterium.
34762581	2	35	attach	isolated	199:206	arg2	strain					165:170	A novel growth-promoting and indole acetic acid-producing strain	107:170	A novel growth-promoting and indole acetic acid-producing strain	107:170	A novel growth-promoting and indole acetic acid-producing strain, designated NEAU-LLBT, was isolated from cow dung collected from Shangzhi, Heilongjiang Province, PR China.
34762581	2	35	attach	isolated	199:206	arg1	cow					213:215	cow dung	213:220	cow dung collected from Shangzhi, Heilongjiang Province, PR China	213:277	A novel growth-promoting and indole acetic acid-producing strain, designated NEAU-LLBT, was isolated from cow dung collected from Shangzhi, Heilongjiang Province, PR China.
34762581	8	36	theme	polar	996:1000	arg1	diphosphatidylglycerol					1014:1035	diphosphatidylglycerol	1014:1035	diphosphatidylglycerol	1014:1035	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid.
34762581	8	36	theme	polar	996:1000	arg1	lipids					1002:1007	The major polar lipids	986:1007	The major polar lipids	986:1007	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unidentified glycolipid.
34762581	9	37	dep	identified	1118:1127	arg1	 0					1145:1146	 0	1145:1146	 0	1145:1146	The major fatty acids were identified as anteiso-C15 : 0, iso-C16 : 0 and iso-C17 : 0.
34762581	11	38	located	found	1428:1432	arg1	addition					1244:1251	addition	1244:1251	addition	1244:1251	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	11	38	located	found	1428:1432	arg2	values					1286:1291	the average nucleotide identity values	1254:1291	the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T,	1254:1421	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	11	38	located	found	1428:1432	arg2	%					1460:1460	81.1, 79.4 and 78.7 %	1440:1460	81.1, 79.4 and 78.7 %	1440:1460	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	12	39	theme	stercoris	1760:1768	arg1	sp					1770:1771	the name Microbacterium stercoris sp	1736:1771	the name Microbacterium stercoris sp	1736:1771	Based on the phenotypic, phylogenetic and genotypic data, strain NEAU-LLBT is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium stercoris sp.
34762581	12	40	theme	Microbacterium	1745:1758	arg1	sp					1770:1771	the name Microbacterium stercoris sp	1736:1771	the name Microbacterium stercoris sp	1736:1771	Based on the phenotypic, phylogenetic and genotypic data, strain NEAU-LLBT is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium stercoris sp.
34762581	13	41	theme	type	1845:1848	arg1	strain					1850:1855	the type strain	1841:1855	the type strain	1841:1855	nov is proposed, with NEAU-LLBT (=CCTCC AA 2018028T=JCM 32660T) as the type strain.
34762581	10	42	theme	G+C	1194:1196	arg1	%					1238:1238	70.2 mol%	1230:1238	70.2 mol%	1230:1238	The genomic DNA G+C content of strain NEAU-LLBT was 70.2 mol%.
34762581	10	42	theme	G+C	1194:1196	arg1	content					1198:1204	The genomic DNA G+C content	1178:1204	The genomic DNA G+C content of strain NEAU-LLBT	1178:1224	The genomic DNA G+C content of strain NEAU-LLBT was 70.2 mol%.
34762581	9	43	dep	 0	1145:1146	arg1	 0					1158:1159	 0	1158:1159	 0	1158:1159	The major fatty acids were identified as anteiso-C15 : 0, iso-C16 : 0 and iso-C17 : 0.
34762581	9	43	dep	 0	1145:1146	arg1	 0					1174:1175	 0	1174:1175	 0	1174:1175	The major fatty acids were identified as anteiso-C15 : 0, iso-C16 : 0 and iso-C17 : 0.
34762581	9	43	dep	 0	1145:1146	arg1	iso-C17 					1165:1172	iso-C17 	1165:1172	iso-C17 	1165:1172	The major fatty acids were identified as anteiso-C15 : 0, iso-C16 : 0 and iso-C17 : 0.
34762581	11	44	theme	marinilacus	1374:1384	arg1	strains					1336:1342	its reference strains	1322:1342	its reference strains	1322:1342	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	11	44	theme	marinilacus	1374:1384	arg1	18904T					1390:1395	M. marinilacus DSM 18904T	1371:1395	M. marinilacus DSM 18904T	1371:1395	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	10	45	theme	genomic	1182:1188	arg1	%					1238:1238	70.2 mol%	1230:1238	70.2 mol%	1230:1238	The genomic DNA G+C content of strain NEAU-LLBT was 70.2 mol%.
34762581	10	45	theme	genomic	1182:1188	arg1	content					1198:1204	The genomic DNA G+C content	1178:1204	The genomic DNA G+C content of strain NEAU-LLBT	1178:1224	The genomic DNA G+C content of strain NEAU-LLBT was 70.2 mol%.
34762581	1	46	theme	indole	38:43	arg1	actinobacterium					67:81	an indole acetic acid-producing actinobacterium	35:81	an indole acetic acid-producing actinobacterium isolated from cow dung	35:104	nov., an indole acetic acid-producing actinobacterium isolated from cow dung.
34762581	2	47	theme	growth-promoting	115:130	arg1	strain					165:170	A novel growth-promoting and indole acetic acid-producing strain	107:170	A novel growth-promoting and indole acetic acid-producing strain	107:170	A novel growth-promoting and indole acetic acid-producing strain, designated NEAU-LLBT, was isolated from cow dung collected from Shangzhi, Heilongjiang Province, PR China.
34762581	7	48	theme	small	914:918	arg1	alanine					930:936	alanine	930:936	alanine	930:936	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	48	theme	small	914:918	arg1	acid					868:871	glutamic acid	859:871	glutamic acid	859:871	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	48	theme	small	914:918	arg1	ornithine					898:906	ornithine	898:906	ornithine	898:906	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	48	theme	small	914:918	arg1	acid					980:983	the diagnostic diamino acid	957:983	the diagnostic diamino acid	957:983	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	48	theme	small	914:918	arg1	acid					883:886	aspartic acid	874:886	aspartic acid	874:886	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	48	theme	small	914:918	arg1	glycine					889:895	glycine	889:895	glycine	889:895	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	48	theme	small	914:918	arg1	amount					920:925	a small amount	912:925	a small amount of alanine	912:936	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	10	49	theme	NEAU-LLBT	1216:1224	arg1	%					1238:1238	70.2 mol%	1230:1238	70.2 mol%	1230:1238	The genomic DNA G+C content of strain NEAU-LLBT was 70.2 mol%.
34762581	10	49	theme	NEAU-LLBT	1216:1224	arg1	content					1198:1204	The genomic DNA G+C content	1178:1204	The genomic DNA G+C content of strain NEAU-LLBT	1178:1224	The genomic DNA G+C content of strain NEAU-LLBT was 70.2 mol%.
34762581	4	50	theme	rRNA	410:413	arg1	sequence					420:427	16S rRNA gene sequence	406:427	16S rRNA gene sequence	406:427	Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain NEAU-LLBT belonged to the genus Microbacterium.
34762581	5	51	dep	Microbacterium	580:593	arg1	paludicola					595:604	paludicola	595:604	paludicola	595:604	Strain NEAU-LLBT had high 16S rRNA sequence similarities of 98.81 and 98.41 % to Microbacterium paludicola DSM 16915T and Microbacterium marinilacus DSM 18904T, and less than 98 % to other members of the genus Microbacterium.
34762581	5	52	theme	%	677:677	arg1	similarities					543:554	high 16S rRNA sequence similarities	520:554	high 16S rRNA sequence similarities of 98.81 and 98.41 % to Microbacterium paludicola DSM 16915T and Microbacterium marinilacus DSM 18904T, and less than 98 % to other members of the genus Microbacterium	520:722	Strain NEAU-LLBT had high 16S rRNA sequence similarities of 98.81 and 98.41 % to Microbacterium paludicola DSM 16915T and Microbacterium marinilacus DSM 18904T, and less than 98 % to other members of the genus Microbacterium.
34762581	2	53	theme	acid-producing	150:163	arg1	strain					165:170	A novel growth-promoting and indole acetic acid-producing strain	107:170	A novel growth-promoting and indole acetic acid-producing strain	107:170	A novel growth-promoting and indole acetic acid-producing strain, designated NEAU-LLBT, was isolated from cow dung collected from Shangzhi, Heilongjiang Province, PR China.
34762581	5	54	theme	18904T	652:657	arg1	similarities					543:554	high 16S rRNA sequence similarities	520:554	high 16S rRNA sequence similarities of 98.81 and 98.41 % to Microbacterium paludicola DSM 16915T and Microbacterium marinilacus DSM 18904T, and less than 98 % to other members of the genus Microbacterium	520:722	Strain NEAU-LLBT had high 16S rRNA sequence similarities of 98.81 and 98.41 % to Microbacterium paludicola DSM 16915T and Microbacterium marinilacus DSM 18904T, and less than 98 % to other members of the genus Microbacterium.
34762581	5	55	theme	high	520:523	arg1	similarities					543:554	high 16S rRNA sequence similarities	520:554	high 16S rRNA sequence similarities of 98.81 and 98.41 % to Microbacterium paludicola DSM 16915T and Microbacterium marinilacus DSM 18904T, and less than 98 % to other members of the genus Microbacterium	520:722	Strain NEAU-LLBT had high 16S rRNA sequence similarities of 98.81 and 98.41 % to Microbacterium paludicola DSM 16915T and Microbacterium marinilacus DSM 18904T, and less than 98 % to other members of the genus Microbacterium.
34762581	2	56	theme	indole	136:141	arg1	strain					165:170	A novel growth-promoting and indole acetic acid-producing strain	107:170	A novel growth-promoting and indole acetic acid-producing strain	107:170	A novel growth-promoting and indole acetic acid-producing strain, designated NEAU-LLBT, was isolated from cow dung collected from Shangzhi, Heilongjiang Province, PR China.
34762581	6	57	theme	predominant	805:815	arg1	MK-12					778:782	MK-12	778:782	MK-12	778:782	Chemotaxonomic characteristics showed that MK-11 and MK-12 were detected as the predominant menaquinones.
34762581	6	57	theme	predominant	805:815	arg1	menaquinones					817:828	the predominant menaquinones	801:828	the predominant menaquinones	801:828	Chemotaxonomic characteristics showed that MK-11 and MK-12 were detected as the predominant menaquinones.
34762581	6	57	theme	predominant	805:815	arg1	MK-11					768:772	MK-11	768:772	MK-11	768:772	Chemotaxonomic characteristics showed that MK-11 and MK-12 were detected as the predominant menaquinones.
34762581	5	58	theme	rRNA	529:532	arg1	similarities					543:554	high 16S rRNA sequence similarities	520:554	high 16S rRNA sequence similarities of 98.81 and 98.41 % to Microbacterium paludicola DSM 16915T and Microbacterium marinilacus DSM 18904T, and less than 98 % to other members of the genus Microbacterium	520:722	Strain NEAU-LLBT had high 16S rRNA sequence similarities of 98.81 and 98.41 % to Microbacterium paludicola DSM 16915T and Microbacterium marinilacus DSM 18904T, and less than 98 % to other members of the genus Microbacterium.
34762581	2	59	dep	Province	260:267	arg1	Heilongjiang					247:258	Shangzhi, Heilongjiang Province	237:267	Heilongjiang	247:258	A novel growth-promoting and indole acetic acid-producing strain, designated NEAU-LLBT, was isolated from cow dung collected from Shangzhi, Heilongjiang Province, PR China.
34762581	5	60	theme	genus	703:707	arg1	Microbacterium					709:722	the genus Microbacterium	699:722	the genus Microbacterium	699:722	Strain NEAU-LLBT had high 16S rRNA sequence similarities of 98.81 and 98.41 % to Microbacterium paludicola DSM 16915T and Microbacterium marinilacus DSM 18904T, and less than 98 % to other members of the genus Microbacterium.
34762581	11	61	theme	DNA-DNA	1502:1508	arg1	hybridization					1510:1522	digital DNA-DNA hybridization	1494:1522	digital DNA-DNA hybridization between them	1494:1535	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	7	62	theme	aspartic	874:881	arg1	alanine					930:936	alanine	930:936	alanine	930:936	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	62	theme	aspartic	874:881	arg1	acid					868:871	glutamic acid	859:871	glutamic acid	859:871	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	62	theme	aspartic	874:881	arg1	ornithine					898:906	ornithine	898:906	ornithine	898:906	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	62	theme	aspartic	874:881	arg1	acid					980:983	the diagnostic diamino acid	957:983	the diagnostic diamino acid	957:983	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	62	theme	aspartic	874:881	arg1	acid					883:886	aspartic acid	874:886	aspartic acid	874:886	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	62	theme	aspartic	874:881	arg1	glycine					889:895	glycine	889:895	glycine	889:895	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	62	theme	aspartic	874:881	arg1	amount					920:925	a small amount	912:925	a small amount of alanine	912:936	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	11	63	theme	DSM	1359:1361	arg1	strains					1336:1342	its reference strains	1322:1342	its reference strains	1322:1342	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	11	63	theme	DSM	1359:1361	arg1	16915T					1363:1368	M. paludicola DSM 16915T	1345:1368	M. paludicola DSM 16915T	1345:1368	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	13	64	theme	=CCTCC AA	1807:1815	arg1	NEAU-LLBT					1796:1804	NEAU-LLBT	1796:1804	NEAU-LLBT (=CCTCC AA 2018028T=JCM 32660T) as the type strain	1796:1855	nov is proposed, with NEAU-LLBT (=CCTCC AA 2018028T=JCM 32660T) as the type strain.
34762581	13	64	theme	=CCTCC AA	1807:1815	arg1	32660T					1830:1835	=CCTCC AA 2018028T=JCM 32660T	1807:1835	=CCTCC AA 2018028T=JCM 32660T	1807:1835	nov is proposed, with NEAU-LLBT (=CCTCC AA 2018028T=JCM 32660T) as the type strain.
34762581	2	65	theme	Shangzhi	237:244	arg1	Province					260:267	Shangzhi, Heilongjiang Province	237:267	Province	260:267	A novel growth-promoting and indole acetic acid-producing strain, designated NEAU-LLBT, was isolated from cow dung collected from Shangzhi, Heilongjiang Province, PR China.
34762581	2	65	theme	Shangzhi	237:244	arg1	China					273:277	PR China	270:277	PR China	270:277	A novel growth-promoting and indole acetic acid-producing strain, designated NEAU-LLBT, was isolated from cow dung collected from Shangzhi, Heilongjiang Province, PR China.
34762581	4	66	theme	genus	477:481	arg1	Microbacterium					483:496	the genus Microbacterium	473:496	the genus Microbacterium	473:496	Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain NEAU-LLBT belonged to the genus Microbacterium.
34762581	11	67	theme	M.	1345:1346	arg1	strains					1336:1342	its reference strains	1322:1342	its reference strains	1322:1342	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	11	67	theme	M.	1345:1346	arg1	16915T					1363:1368	M. paludicola DSM 16915T	1345:1368	M. paludicola DSM 16915T	1345:1368	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	5	68	theme	other	682:686	arg1	members					688:694	other members	682:694	other members of the genus Microbacterium	682:722	Strain NEAU-LLBT had high 16S rRNA sequence similarities of 98.81 and 98.41 % to Microbacterium paludicola DSM 16915T and Microbacterium marinilacus DSM 18904T, and less than 98 % to other members of the genus Microbacterium.
34762581	3	69	theme	strain	289:294	arg1	NEAU-LLBT					296:304	strain NEAU-LLBT	289:304	strain NEAU-LLBT	289:304	Cells of strain NEAU-LLBT were Gram-stain-positive, non-motile, aerobic and non-spore-forming.
34762581	10	70	theme	70.2 mol	1230:1237	arg1	%					1238:1238	70.2 mol%	1230:1238	70.2 mol%	1230:1238	The genomic DNA G+C content of strain NEAU-LLBT was 70.2 mol%.
34762581	10	70	theme	70.2 mol	1230:1237	arg1	content					1198:1204	The genomic DNA G+C content	1178:1204	The genomic DNA G+C content of strain NEAU-LLBT	1178:1224	The genomic DNA G+C content of strain NEAU-LLBT was 70.2 mol%.
34762581	13	71	theme	2018028T=JCM	1817:1828	arg1	NEAU-LLBT					1796:1804	NEAU-LLBT	1796:1804	NEAU-LLBT (=CCTCC AA 2018028T=JCM 32660T) as the type strain	1796:1855	nov is proposed, with NEAU-LLBT (=CCTCC AA 2018028T=JCM 32660T) as the type strain.
34762581	13	71	theme	2018028T=JCM	1817:1828	arg1	32660T					1830:1835	=CCTCC AA 2018028T=JCM 32660T	1807:1835	=CCTCC AA 2018028T=JCM 32660T	1807:1835	nov is proposed, with NEAU-LLBT (=CCTCC AA 2018028T=JCM 32660T) as the type strain.
34762581	12	72	theme	phylogenetic	1604:1615	arg1	data					1631:1634	the phenotypic, phylogenetic and genotypic data	1588:1634	the phenotypic, phylogenetic and genotypic data	1588:1634	Based on the phenotypic, phylogenetic and genotypic data, strain NEAU-LLBT is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium stercoris sp.
34762581	9	73	theme	major	1095:1099	arg1	acids					1107:1111	The major fatty acids	1091:1111	The major fatty acids	1091:1111	The major fatty acids were identified as anteiso-C15 : 0, iso-C16 : 0 and iso-C17 : 0.
34762581	9	73	theme	major	1095:1099	arg1	anteiso-C15 					1132:1143	anteiso-C15 	1132:1143	anteiso-C15 	1132:1143	The major fatty acids were identified as anteiso-C15 : 0, iso-C16 : 0 and iso-C17 : 0.
34762581	5	74	theme	Microbacterium	621:634	arg1	18904T					652:657	Microbacterium marinilacus DSM 18904T	621:657	Microbacterium marinilacus DSM 18904T	621:657	Strain NEAU-LLBT had high 16S rRNA sequence similarities of 98.81 and 98.41 % to Microbacterium paludicola DSM 16915T and Microbacterium marinilacus DSM 18904T, and less than 98 % to other members of the genus Microbacterium.
34762581	1	75	theme	acetic	45:50	arg1	actinobacterium					67:81	an indole acetic acid-producing actinobacterium	35:81	an indole acetic acid-producing actinobacterium isolated from cow dung	35:104	nov., an indole acetic acid-producing actinobacterium isolated from cow dung.
34762581	12	76	theme	genotypic	1621:1629	arg1	data					1631:1634	the phenotypic, phylogenetic and genotypic data	1588:1634	the phenotypic, phylogenetic and genotypic data	1588:1634	Based on the phenotypic, phylogenetic and genotypic data, strain NEAU-LLBT is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium stercoris sp.
34762581	12	77	theme	Microbacterium	1710:1723	arg1	species					1689:1695	a novel species	1681:1695	a novel species	1681:1695	Based on the phenotypic, phylogenetic and genotypic data, strain NEAU-LLBT is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium stercoris sp.
34762581	11	78	theme	nucleotide	1266:1275	arg1	values					1286:1291	the average nucleotide identity values	1254:1291	the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T,	1254:1421	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	11	78	theme	nucleotide	1266:1275	arg1	%					1460:1460	81.1, 79.4 and 78.7 %	1440:1460	81.1, 79.4 and 78.7 %	1440:1460	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	7	79	theme	glutamic	859:866	arg1	alanine					930:936	alanine	930:936	alanine	930:936	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	79	theme	glutamic	859:866	arg1	acid					868:871	glutamic acid	859:871	glutamic acid	859:871	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	79	theme	glutamic	859:866	arg1	ornithine					898:906	ornithine	898:906	ornithine	898:906	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	79	theme	glutamic	859:866	arg1	acid					980:983	the diagnostic diamino acid	957:983	the diagnostic diamino acid	957:983	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	79	theme	glutamic	859:866	arg1	acid					883:886	aspartic acid	874:886	aspartic acid	874:886	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	79	theme	glutamic	859:866	arg1	glycine					889:895	glycine	889:895	glycine	889:895	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	79	theme	glutamic	859:866	arg1	amount					920:925	a small amount	912:925	a small amount of alanine	912:936	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	6	80	theme	Chemotaxonomic	725:738	arg1	characteristics					740:754	Chemotaxonomic characteristics	725:754	Chemotaxonomic characteristics	725:754	Chemotaxonomic characteristics showed that MK-11 and MK-12 were detected as the predominant menaquinones.
34762581	5	81	theme	DSM	648:650	arg1	18904T					652:657	Microbacterium marinilacus DSM 18904T	621:657	Microbacterium marinilacus DSM 18904T	621:657	Strain NEAU-LLBT had high 16S rRNA sequence similarities of 98.81 and 98.41 % to Microbacterium paludicola DSM 16915T and Microbacterium marinilacus DSM 18904T, and less than 98 % to other members of the genus Microbacterium.
34762581	5	82	contain	had	516:518	arg1	NEAU-LLBT					506:514	Strain NEAU-LLBT	499:514	Strain NEAU-LLBT	499:514	Strain NEAU-LLBT had high 16S rRNA sequence similarities of 98.81 and 98.41 % to Microbacterium paludicola DSM 16915T and Microbacterium marinilacus DSM 18904T, and less than 98 % to other members of the genus Microbacterium.
34762581	5	82	contain	had	516:518	arg2	similarities					543:554	high 16S rRNA sequence similarities	520:554	high 16S rRNA sequence similarities of 98.81 and 98.41 % to Microbacterium paludicola DSM 16915T and Microbacterium marinilacus DSM 18904T, and less than 98 % to other members of the genus Microbacterium	520:722	Strain NEAU-LLBT had high 16S rRNA sequence similarities of 98.81 and 98.41 % to Microbacterium paludicola DSM 16915T and Microbacterium marinilacus DSM 18904T, and less than 98 % to other members of the genus Microbacterium.
34762581	0	83	theme	stercoris	15:23	arg1	sp					25:26	Microbacterium stercoris sp	0:26	Microbacterium stercoris sp.	0:27	Microbacterium stercoris sp.
34762581	12	84	theme	phenotypic	1592:1601	arg1	data					1631:1634	the phenotypic, phylogenetic and genotypic data	1588:1634	the phenotypic, phylogenetic and genotypic data	1588:1634	Based on the phenotypic, phylogenetic and genotypic data, strain NEAU-LLBT is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium stercoris sp.
34762581	11	85	theme	strain	1301:1306	arg1	NEAU-LLBT					1308:1316	strain NEAU-LLBT	1301:1316	strain NEAU-LLBT	1301:1316	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	7	86	theme	diamino	972:978	arg1	alanine					930:936	alanine	930:936	alanine	930:936	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	86	theme	diamino	972:978	arg1	acid					868:871	glutamic acid	859:871	glutamic acid	859:871	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	86	theme	diamino	972:978	arg1	ornithine					898:906	ornithine	898:906	ornithine	898:906	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	86	theme	diamino	972:978	arg1	acid					980:983	the diagnostic diamino acid	957:983	the diagnostic diamino acid	957:983	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	86	theme	diamino	972:978	arg1	acid					883:886	aspartic acid	874:886	aspartic acid	874:886	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	86	theme	diamino	972:978	arg1	glycine					889:895	glycine	889:895	glycine	889:895	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	86	theme	diamino	972:978	arg1	amount					920:925	a small amount	912:925	a small amount of alanine	912:936	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	1	87	theme	dung	101:104	arg1	cow					97:99	cow dung	97:104	cow dung	97:104	nov., an indole acetic acid-producing actinobacterium isolated from cow dung.
34762581	11	88	theme	23.8	1542:1545	arg1	level					1485:1489	the level	1481:1489	the level of digital DNA-DNA hybridization between them	1481:1535	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	11	88	theme	23.8	1542:1545	arg1	%					1562:1562	23.8, 22.6 and 21.8 %	1542:1562	%	1562:1562	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	7	89	theme	alanine	930:936	arg1	alanine					930:936	alanine	930:936	alanine	930:936	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	89	theme	alanine	930:936	arg1	acid					868:871	glutamic acid	859:871	glutamic acid	859:871	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	89	theme	alanine	930:936	arg1	ornithine					898:906	ornithine	898:906	ornithine	898:906	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	89	theme	alanine	930:936	arg1	acid					980:983	the diagnostic diamino acid	957:983	the diagnostic diamino acid	957:983	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	89	theme	alanine	930:936	arg1	acid					883:886	aspartic acid	874:886	aspartic acid	874:886	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	89	theme	alanine	930:936	arg1	glycine					889:895	glycine	889:895	glycine	889:895	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	7	89	theme	alanine	930:936	arg1	amount					920:925	a small amount	912:925	a small amount of alanine	912:936	The peptidoglycan contained glutamic acid, aspartic acid, glycine, ornithine and a small amount of alanine, with ornithine as the diagnostic diamino acid.
34762581	12	90	theme	name	1740:1743	arg1	sp					1770:1771	the name Microbacterium stercoris sp	1736:1771	the name Microbacterium stercoris sp	1736:1771	Based on the phenotypic, phylogenetic and genotypic data, strain NEAU-LLBT is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium stercoris sp.
34762581	11	91	theme	21.8 	1557:1561	arg1	level					1485:1489	the level	1481:1489	the level of digital DNA-DNA hybridization between them	1481:1535	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	11	91	theme	21.8 	1557:1561	arg1	%					1562:1562	23.8, 22.6 and 21.8 %	1542:1562	%	1562:1562	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	10	92	theme	DNA	1190:1192	arg1	%					1238:1238	70.2 mol%	1230:1238	70.2 mol%	1230:1238	The genomic DNA G+C content of strain NEAU-LLBT was 70.2 mol%.
34762581	10	92	theme	DNA	1190:1192	arg1	content					1198:1204	The genomic DNA G+C content	1178:1204	The genomic DNA G+C content of strain NEAU-LLBT	1178:1224	The genomic DNA G+C content of strain NEAU-LLBT was 70.2 mol%.
34762581	12	93	theme	genus	1704:1708	arg1	Microbacterium					1710:1723	the genus Microbacterium	1700:1723	the genus Microbacterium	1700:1723	Based on the phenotypic, phylogenetic and genotypic data, strain NEAU-LLBT is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium stercoris sp.
34762581	5	94	dep	Microbacterium	621:634	arg1	marinilacus					636:646	marinilacus	636:646	marinilacus	636:646	Strain NEAU-LLBT had high 16S rRNA sequence similarities of 98.81 and 98.41 % to Microbacterium paludicola DSM 16915T and Microbacterium marinilacus DSM 18904T, and less than 98 % to other members of the genus Microbacterium.
34762581	11	95	theme	DSM	1386:1388	arg1	strains					1336:1342	its reference strains	1322:1342	its reference strains	1322:1342	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
34762581	11	95	theme	DSM	1386:1388	arg1	18904T					1390:1395	M. marinilacus DSM 18904T	1371:1395	M. marinilacus DSM 18904T	1371:1395	In addition, the average nucleotide identity values between strain NEAU-LLBT and its reference strains, M. paludicola DSM 16915T, M. marinilacus DSM 18904T and M. album SYSU D8007T, were found to be 81.1, 79.4 and 78.7 %, respectively, and the level of digital DNA-DNA hybridization between them were 23.8, 22.6 and 21.8 %, respectively.
32598858	7	0	theme	chain	1368:1372	arg1	composition					1374:1384	α-myosin heavy chain composition	1353:1384	α-myosin heavy chain composition (p = 0.074)	1353:1396	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	7	0	theme	chain	1368:1372	arg1	0.074					1391:1395	p = 0.074	1387:1395	p = 0.074	1387:1395	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	7	1	theme	HFHS+Sed	1150:1157	arg1	group					1159:1163	the HFHS+Sed group	1146:1163	the HFHS+Sed group	1146:1163	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	7	2	theme	α-myosin	1353:1360	arg1	chain					1368:1372	α-myosin heavy chain	1353:1372	α-myosin heavy chain composition (p = 0.074)	1353:1396	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	9	3	theme	Novelty	1542:1548	arg1	muscle					1558:1563	Novelty Cardiac muscle	1542:1563	Novelty Cardiac muscle from animals exposed to an obesogenic diet for 24 weeks	1542:1619	Novelty Cardiac muscle from animals exposed to an obesogenic diet for 24 weeks had impaired contractile properties compared with controls.
32598858	8	4	theme	contractile	1518:1528	arg1	properties					1530:1539	contractile properties	1518:1539	contractile properties	1518:1539	However, 12 weeks of moderate aerobic exercise partially prevented these decrements in contractile properties.
32598858	4	5	dep	sedentary	709:717	arg1	HFHS+Sed					720:727	HFHS+Sed	720:727	HFHS+Sed	720:727	Following 12 weeks of obesity induction the HFHS group animals were stratified and grouped into sedentary (HFHS+Sed) and exercise (HFHS+Ex) groups for an additional 12 weeks.
32598858	5	6	theme	diet	810:813	arg1	intervention					815:826	diet intervention	810:826	diet intervention	810:826	Following 24 weeks of diet intervention, with 12 weeks of aerobic exercise (25 m/min, 30 min/day, 5 days/week) for the HFHS+Ex group, skinned cardiac fibre bundle testing was used to evaluate cardiac contractile properties.
32598858	7	7	theme	calcium	1295:1301	arg1	sensitivity					1303:1313	lower calcium sensitivity	1289:1313	lower calcium sensitivity (p = 0.077)	1289:1325	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	7	7	theme	calcium	1295:1301	arg1	0.077					1320:1324	p = 0.077	1316:1324	p = 0.077	1316:1324	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	3	8	theme	chow	556:559	arg1	diet					561:564	a chow diet	554:564	a chow diet	554:564	Twelve-week-old rats (n = 30) were organized into either a chow diet or a high-fat, high-sucrose (HFHS) diet group.
32598858	9	9	theme	obesogenic	1592:1601	arg1	diet					1603:1606	an obesogenic diet	1589:1606	an obesogenic diet	1589:1606	Novelty Cardiac muscle from animals exposed to an obesogenic diet for 24 weeks had impaired contractile properties compared with controls.
32598858	10	10	theme	contractile	1711:1721	arg1	properties					1723:1732	contractile properties	1711:1732	contractile properties of the heart	1711:1745	Obesity-induced impairment of contractile properties of the heart were partially prevented by a 12-week aerobic exercise regime.
32598858	7	11	from	rats	1138:1141	arg1	Hearts					1126:1131	Hearts	1126:1131	Hearts from rats in the HFHS+Sed group	1126:1163	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	7	11	from	rats	1138:1141	arg1	group					1159:1163	the HFHS+Sed group	1146:1163	the HFHS+Sed group	1146:1163	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	5	12	theme	cardiac	980:986	arg1	properties					1000:1009	cardiac contractile properties	980:1009	cardiac contractile properties	980:1009	Following 24 weeks of diet intervention, with 12 weeks of aerobic exercise (25 m/min, 30 min/day, 5 days/week) for the HFHS+Ex group, skinned cardiac fibre bundle testing was used to evaluate cardiac contractile properties.
32598858	3	13	theme	HFHS	595:598	arg1	diet					601:604	a high-fat, high-sucrose (HFHS) diet	569:604	diet	601:604	Twelve-week-old rats (n = 30) were organized into either a chow diet or a high-fat, high-sucrose (HFHS) diet group.
32598858	9	14	theme	impaired	1625:1632	arg1	properties					1646:1655	impaired contractile properties	1625:1655	impaired contractile properties	1625:1655	Novelty Cardiac muscle from animals exposed to an obesogenic diet for 24 weeks had impaired contractile properties compared with controls.
32598858	4	15	theme	induction	643:651	arg1	weeks					626:630	12 weeks	623:630	12 weeks of obesity induction	623:651	Following 12 weeks of obesity induction the HFHS group animals were stratified and grouped into sedentary (HFHS+Sed) and exercise (HFHS+Ex) groups for an additional 12 weeks.
32598858	1	16	theme	cardiac	310:316	arg1	function					337:344	cardiac muscle contractile function	310:344	cardiac muscle contractile function	310:344	Decrements in contractile function resulting from obesity are thought to be major reasons for the link between obesity and cardiovascular disease, while exercise has been shown to improve cardiac muscle contractile function.
32598858	1	17	theme	contractile	136:146	arg1	function					148:155	contractile function	136:155	contractile function resulting from obesity	136:178	Decrements in contractile function resulting from obesity are thought to be major reasons for the link between obesity and cardiovascular disease, while exercise has been shown to improve cardiac muscle contractile function.
32598858	2	18	theme	exercise	487:494	arg1	induction					438:446	obesity induction	430:446	obesity induction	430:446	The purpose of this study was to evaluate cardiac contractile properties following obesity induction and the potential protective effect of exercise.
32598858	2	18	theme	exercise	487:494	arg1	effect					477:482	the potential protective effect	452:482	the potential protective effect of exercise	452:494	The purpose of this study was to evaluate cardiac contractile properties following obesity induction and the potential protective effect of exercise.
32598858	4	19	theme	HFHS	657:660	arg1	animals					668:674	the HFHS group animals	653:674	the HFHS group animals	653:674	Following 12 weeks of obesity induction the HFHS group animals were stratified and grouped into sedentary (HFHS+Sed) and exercise (HFHS+Ex) groups for an additional 12 weeks.
32598858	1	20	theme	contractile	325:335	arg1	function					337:344	cardiac muscle contractile function	310:344	cardiac muscle contractile function	310:344	Decrements in contractile function resulting from obesity are thought to be major reasons for the link between obesity and cardiovascular disease, while exercise has been shown to improve cardiac muscle contractile function.
32598858	5	21	theme	aerobic	846:852	arg1	exercise					854:861	aerobic exercise	846:861	aerobic exercise (25 m/min, 30 min/day, 5 days/week) for the HFHS+Ex group	846:919	Following 24 weeks of diet intervention, with 12 weeks of aerobic exercise (25 m/min, 30 min/day, 5 days/week) for the HFHS+Ex group, skinned cardiac fibre bundle testing was used to evaluate cardiac contractile properties.
32598858	10	22	theme	heart	1741:1745	arg1	properties					1723:1732	contractile properties	1711:1732	contractile properties of the heart	1711:1745	Obesity-induced impairment of contractile properties of the heart were partially prevented by a 12-week aerobic exercise regime.
32598858	5	23	dep	exercise	854:861	arg1	5 days/week					886:896	5 days/week	886:896	5 days/week	886:896	Following 24 weeks of diet intervention, with 12 weeks of aerobic exercise (25 m/min, 30 min/day, 5 days/week) for the HFHS+Ex group, skinned cardiac fibre bundle testing was used to evaluate cardiac contractile properties.
32598858	5	23	dep	exercise	854:861	arg1	30 min/day					874:883	30 min/day	874:883	30 min/day	874:883	Following 24 weeks of diet intervention, with 12 weeks of aerobic exercise (25 m/min, 30 min/day, 5 days/week) for the HFHS+Ex group, skinned cardiac fibre bundle testing was used to evaluate cardiac contractile properties.
32598858	5	23	dep	exercise	854:861	arg1	25 m/min					864:871	25 m/min	864:871	25 m/min	864:871	Following 24 weeks of diet intervention, with 12 weeks of aerobic exercise (25 m/min, 30 min/day, 5 days/week) for the HFHS+Ex group, skinned cardiac fibre bundle testing was used to evaluate cardiac contractile properties.
32598858	0	24	theme	diet-induced	92:103	arg1	obesity					105:111	diet-induced obesity	92:111	diet-induced obesity in rats	92:119	Mechanical function of cardiac fibre bundles is partly protected by exercise in response to diet-induced obesity in rats.
32598858	6	25	theme	Body	1012:1015	arg1	fat					1017:1019	Body fat	1012:1019	Body fat	1012:1019	Body fat and mass were significantly greater in the HFHS-fed animals compared with the chow controls (p < 0.043).
32598858	2	26	theme	study	367:371	arg1	purpose					351:357	The purpose	347:357	The purpose of this study	347:371	The purpose of this study was to evaluate cardiac contractile properties following obesity induction and the potential protective effect of exercise.
32598858	0	27	theme	Mechanical	0:9	arg1	function					11:18	Mechanical function	0:18	Mechanical function of cardiac fibre bundles	0:43	Mechanical function of cardiac fibre bundles is partly protected by exercise in response to diet-induced obesity in rats.
32598858	7	28	theme	maximum	1229:1235	arg1	mass					1191:1194	significantly greater mass	1169:1194	significantly greater mass (p < 0.03)	1169:1205	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	7	28	theme	maximum	1229:1235	arg1	p =					1258:1260	p = 0.001	1258:1266	p = 0.001	1258:1266	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	7	28	theme	maximum	1229:1235	arg1	velocity					1248:1255	significantly slower maximum shortening velocity	1208:1255	significantly slower maximum shortening velocity (p = 0.001)	1208:1267	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	2	29	theme	obesity	430:436	arg1	induction					438:446	obesity induction	430:446	obesity induction	430:446	The purpose of this study was to evaluate cardiac contractile properties following obesity induction and the potential protective effect of exercise.
32598858	2	30	theme	cardiac	389:395	arg1	properties					409:418	cardiac contractile properties	389:418	cardiac contractile properties	389:418	The purpose of this study was to evaluate cardiac contractile properties following obesity induction and the potential protective effect of exercise.
32598858	0	31	theme	fibre	31:35	arg1	bundles					37:43	cardiac fibre bundles	23:43	cardiac fibre bundles	23:43	Mechanical function of cardiac fibre bundles is partly protected by exercise in response to diet-induced obesity in rats.
32598858	2	32	theme	potential	456:464	arg1	effect					477:482	the potential protective effect	452:482	the potential protective effect of exercise	452:494	The purpose of this study was to evaluate cardiac contractile properties following obesity induction and the potential protective effect of exercise.
32598858	3	33	theme	diet	601:604	arg1	group					606:610	a high-fat, high-sucrose (HFHS) diet group	569:610	a high-fat, high-sucrose (HFHS) diet group	569:610	Twelve-week-old rats (n = 30) were organized into either a chow diet or a high-fat, high-sucrose (HFHS) diet group.
32598858	4	34	theme	additional	767:776	arg1	12 weeks					778:785	an additional 12 weeks	764:785	an additional 12 weeks	764:785	Following 12 weeks of obesity induction the HFHS group animals were stratified and grouped into sedentary (HFHS+Sed) and exercise (HFHS+Ex) groups for an additional 12 weeks.
32598858	10	35	theme	exercise	1793:1800	arg1	regime					1802:1807	a 12-week aerobic exercise regime	1775:1807	a 12-week aerobic exercise regime	1775:1807	Obesity-induced impairment of contractile properties of the heart were partially prevented by a 12-week aerobic exercise regime.
32598858	7	36	theme	composition	1374:1384	arg1	proportion					1339:1348	a lower proportion	1331:1348	a lower proportion of α-myosin heavy chain composition (p = 0.074)	1331:1396	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	7	36	theme	composition	1374:1384	arg1	sensitivity					1303:1313	lower calcium sensitivity	1289:1313	lower calcium sensitivity (p = 0.077)	1289:1325	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	7	36	theme	composition	1374:1384	arg1	0.077					1320:1324	p = 0.077	1316:1324	p = 0.077	1316:1324	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	5	37	theme	skinned	922:928	arg1	testing					951:957	skinned cardiac fibre bundle testing	922:957	skinned cardiac fibre bundle testing	922:957	Following 24 weeks of diet intervention, with 12 weeks of aerobic exercise (25 m/min, 30 min/day, 5 days/week) for the HFHS+Ex group, skinned cardiac fibre bundle testing was used to evaluate cardiac contractile properties.
32598858	8	38	theme	aerobic	1461:1467	arg1	exercise					1469:1476	moderate aerobic exercise	1452:1476	moderate aerobic exercise	1452:1476	However, 12 weeks of moderate aerobic exercise partially prevented these decrements in contractile properties.
32598858	10	39	theme	12-week	1777:1783	arg1	regime					1802:1807	a 12-week aerobic exercise regime	1775:1807	a 12-week aerobic exercise regime	1775:1807	Obesity-induced impairment of contractile properties of the heart were partially prevented by a 12-week aerobic exercise regime.
32598858	9	40	contain	had	1621:1623	arg1	muscle					1558:1563	Novelty Cardiac muscle	1542:1563	Novelty Cardiac muscle from animals exposed to an obesogenic diet for 24 weeks	1542:1619	Novelty Cardiac muscle from animals exposed to an obesogenic diet for 24 weeks had impaired contractile properties compared with controls.
32598858	9	40	contain	had	1621:1623	arg2	properties					1646:1655	impaired contractile properties	1625:1655	impaired contractile properties	1625:1655	Novelty Cardiac muscle from animals exposed to an obesogenic diet for 24 weeks had impaired contractile properties compared with controls.
32598858	7	41	theme	heavy	1362:1366	arg1	chain					1368:1372	α-myosin heavy chain	1353:1372	α-myosin heavy chain composition (p = 0.074)	1353:1396	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	5	42	theme	fibre	938:942	arg1	testing					951:957	skinned cardiac fibre bundle testing	922:957	skinned cardiac fibre bundle testing	922:957	Following 24 weeks of diet intervention, with 12 weeks of aerobic exercise (25 m/min, 30 min/day, 5 days/week) for the HFHS+Ex group, skinned cardiac fibre bundle testing was used to evaluate cardiac contractile properties.
32598858	1	43	theme	cardiovascular	245:258	arg1	disease					260:266	cardiovascular disease	245:266	cardiovascular disease	245:266	Decrements in contractile function resulting from obesity are thought to be major reasons for the link between obesity and cardiovascular disease, while exercise has been shown to improve cardiac muscle contractile function.
32598858	7	44	theme	lower	1333:1337	arg1	proportion					1339:1348	a lower proportion	1331:1348	a lower proportion of α-myosin heavy chain composition (p = 0.074)	1331:1396	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	9	45	theme	Cardiac	1550:1556	arg1	muscle					1558:1563	Novelty Cardiac muscle	1542:1563	Novelty Cardiac muscle from animals exposed to an obesogenic diet for 24 weeks	1542:1619	Novelty Cardiac muscle from animals exposed to an obesogenic diet for 24 weeks had impaired contractile properties compared with controls.
32598858	6	46	theme	chow	1099:1102	arg1	controls					1104:1111	the chow controls	1095:1111	the chow controls (p < 0.043)	1095:1123	Body fat and mass were significantly greater in the HFHS-fed animals compared with the chow controls (p < 0.043).
32598858	6	46	theme	chow	1099:1102	arg1	0.043					1118:1122	p < 0.043	1114:1122	p < 0.043	1114:1122	Body fat and mass were significantly greater in the HFHS-fed animals compared with the chow controls (p < 0.043).
32598858	10	47	theme	Obesity-induced	1681:1695	arg1	impairment					1697:1706	Obesity-induced impairment	1681:1706	Obesity-induced impairment of contractile properties of the heart	1681:1745	Obesity-induced impairment of contractile properties of the heart were partially prevented by a 12-week aerobic exercise regime.
32598858	5	48	theme	intervention	815:826	arg1	24 weeks					798:805	24 weeks	798:805	24 weeks of diet intervention	798:826	Following 24 weeks of diet intervention, with 12 weeks of aerobic exercise (25 m/min, 30 min/day, 5 days/week) for the HFHS+Ex group, skinned cardiac fibre bundle testing was used to evaluate cardiac contractile properties.
32598858	9	49	from	animals	1570:1576	arg1	muscle					1558:1563	Novelty Cardiac muscle	1542:1563	Novelty Cardiac muscle from animals exposed to an obesogenic diet for 24 weeks	1542:1619	Novelty Cardiac muscle from animals exposed to an obesogenic diet for 24 weeks had impaired contractile properties compared with controls.
32598858	7	50	theme	lower	1289:1293	arg1	sensitivity					1303:1313	lower calcium sensitivity	1289:1313	lower calcium sensitivity (p = 0.077)	1289:1325	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	7	50	theme	lower	1289:1293	arg1	0.077					1320:1324	p = 0.077	1316:1324	p = 0.077	1316:1324	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	10	51	theme	properties	1723:1732	arg1	impairment					1697:1706	Obesity-induced impairment	1681:1706	Obesity-induced impairment of contractile properties of the heart	1681:1745	Obesity-induced impairment of contractile properties of the heart were partially prevented by a 12-week aerobic exercise regime.
32598858	1	52	theme	major	198:202	arg1	reasons					204:210	major reasons	198:210	major reasons for the link between obesity and cardiovascular disease	198:266	Decrements in contractile function resulting from obesity are thought to be major reasons for the link between obesity and cardiovascular disease, while exercise has been shown to improve cardiac muscle contractile function.
32598858	5	53	theme	exercise	854:861	arg1	12 weeks					834:841	12 weeks	834:841	12 weeks of aerobic exercise (25 m/min, 30 min/day, 5 days/week) for the HFHS+Ex group	834:919	Following 24 weeks of diet intervention, with 12 weeks of aerobic exercise (25 m/min, 30 min/day, 5 days/week) for the HFHS+Ex group, skinned cardiac fibre bundle testing was used to evaluate cardiac contractile properties.
32598858	0	54	from	obesity	105:111	arg1	rats					116:119	rats	116:119	rats	116:119	Mechanical function of cardiac fibre bundles is partly protected by exercise in response to diet-induced obesity in rats.
32598858	4	55	theme	group	662:666	arg1	animals					668:674	the HFHS group animals	653:674	the HFHS group animals	653:674	Following 12 weeks of obesity induction the HFHS group animals were stratified and grouped into sedentary (HFHS+Sed) and exercise (HFHS+Ex) groups for an additional 12 weeks.
32598858	5	56	theme	contractile	988:998	arg1	properties					1000:1009	cardiac contractile properties	980:1009	cardiac contractile properties	980:1009	Following 24 weeks of diet intervention, with 12 weeks of aerobic exercise (25 m/min, 30 min/day, 5 days/week) for the HFHS+Ex group, skinned cardiac fibre bundle testing was used to evaluate cardiac contractile properties.
32598858	9	57	theme	contractile	1634:1644	arg1	properties					1646:1655	impaired contractile properties	1625:1655	impaired contractile properties	1625:1655	Novelty Cardiac muscle from animals exposed to an obesogenic diet for 24 weeks had impaired contractile properties compared with controls.
32598858	4	58	theme	obesity	635:641	arg1	induction					643:651	obesity induction	635:651	obesity induction	635:651	Following 12 weeks of obesity induction the HFHS group animals were stratified and grouped into sedentary (HFHS+Sed) and exercise (HFHS+Ex) groups for an additional 12 weeks.
32598858	1	59	theme	muscle	318:323	arg1	function					337:344	cardiac muscle contractile function	310:344	cardiac muscle contractile function	310:344	Decrements in contractile function resulting from obesity are thought to be major reasons for the link between obesity and cardiovascular disease, while exercise has been shown to improve cardiac muscle contractile function.
32598858	7	60	contain	had	1165:1167	arg2	velocity					1248:1255	significantly slower maximum shortening velocity	1208:1255	significantly slower maximum shortening velocity (p = 0.001)	1208:1267	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	7	60	contain	had	1165:1167	arg2	p <					1197:1199	p < 0.03	1197:1204	p < 0.03	1197:1204	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	7	60	contain	had	1165:1167	arg2	mass					1191:1194	significantly greater mass	1169:1194	significantly greater mass (p < 0.03)	1169:1205	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	7	60	contain	had	1165:1167	arg1	Hearts					1126:1131	Hearts	1126:1131	Hearts from rats in the HFHS+Sed group	1126:1163	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	0	61	dep	obesity	105:111	arg1	response					80:87	response	80:87	response	80:87	Mechanical function of cardiac fibre bundles is partly protected by exercise in response to diet-induced obesity in rats.
32598858	7	62	theme	chow	1417:1420	arg1	animals					1422:1428	the sedentary chow animals	1403:1428	the sedentary chow animals	1403:1428	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	5	63	theme	HFHS+Ex	907:913	arg1	group					915:919	the HFHS+Ex group	903:919	the HFHS+Ex group	903:919	Following 24 weeks of diet intervention, with 12 weeks of aerobic exercise (25 m/min, 30 min/day, 5 days/week) for the HFHS+Ex group, skinned cardiac fibre bundle testing was used to evaluate cardiac contractile properties.
32598858	7	64	theme	shortening	1237:1246	arg1	mass					1191:1194	significantly greater mass	1169:1194	significantly greater mass (p < 0.03)	1169:1205	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	7	64	theme	shortening	1237:1246	arg1	p =					1258:1260	p = 0.001	1258:1266	p = 0.001	1258:1266	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	7	64	theme	shortening	1237:1246	arg1	velocity					1248:1255	significantly slower maximum shortening velocity	1208:1255	significantly slower maximum shortening velocity (p = 0.001)	1208:1267	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	1	65	from	Decrements	122:131	arg1	function					148:155	contractile function	136:155	contractile function resulting from obesity	136:178	Decrements in contractile function resulting from obesity are thought to be major reasons for the link between obesity and cardiovascular disease, while exercise has been shown to improve cardiac muscle contractile function.
32598858	7	66	theme	slower	1222:1227	arg1	mass					1191:1194	significantly greater mass	1169:1194	significantly greater mass (p < 0.03)	1169:1205	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	7	66	theme	slower	1222:1227	arg1	p =					1258:1260	p = 0.001	1258:1266	p = 0.001	1258:1266	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	7	66	theme	slower	1222:1227	arg1	velocity					1248:1255	significantly slower maximum shortening velocity	1208:1255	significantly slower maximum shortening velocity (p = 0.001)	1208:1267	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	0	67	theme	cardiac	23:29	arg1	bundles					37:43	cardiac fibre bundles	23:43	cardiac fibre bundles	23:43	Mechanical function of cardiac fibre bundles is partly protected by exercise in response to diet-induced obesity in rats.
32598858	7	68	contain	have	1284:1287	arg2	proportion					1339:1348	a lower proportion	1331:1348	a lower proportion of α-myosin heavy chain composition (p = 0.074)	1331:1396	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	7	68	contain	have	1284:1287	arg2	0.077					1320:1324	p = 0.077	1316:1324	p = 0.077	1316:1324	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	7	68	contain	have	1284:1287	arg1	Hearts					1126:1131	Hearts	1126:1131	Hearts from rats in the HFHS+Sed group	1126:1163	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	7	68	contain	have	1284:1287	arg2	sensitivity					1303:1313	lower calcium sensitivity	1289:1313	lower calcium sensitivity (p = 0.077)	1289:1325	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	2	69	theme	contractile	397:407	arg1	properties					409:418	cardiac contractile properties	389:418	cardiac contractile properties	389:418	The purpose of this study was to evaluate cardiac contractile properties following obesity induction and the potential protective effect of exercise.
32598858	0	70	theme	bundles	37:43	arg1	function					11:18	Mechanical function	0:18	Mechanical function of cardiac fibre bundles	0:43	Mechanical function of cardiac fibre bundles is partly protected by exercise in response to diet-induced obesity in rats.
32598858	7	71	theme	sedentary	1407:1415	arg1	animals					1422:1428	the sedentary chow animals	1403:1428	the sedentary chow animals	1403:1428	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	5	72	theme	bundle	944:949	arg1	testing					951:957	skinned cardiac fibre bundle testing	922:957	skinned cardiac fibre bundle testing	922:957	Following 24 weeks of diet intervention, with 12 weeks of aerobic exercise (25 m/min, 30 min/day, 5 days/week) for the HFHS+Ex group, skinned cardiac fibre bundle testing was used to evaluate cardiac contractile properties.
32598858	2	73	theme	protective	466:475	arg1	effect					477:482	the potential protective effect	452:482	the potential protective effect of exercise	452:494	The purpose of this study was to evaluate cardiac contractile properties following obesity induction and the potential protective effect of exercise.
32598858	6	74	theme	HFHS-fed	1064:1071	arg1	animals					1073:1079	the HFHS-fed animals	1060:1079	the HFHS-fed animals	1060:1079	Body fat and mass were significantly greater in the HFHS-fed animals compared with the chow controls (p < 0.043).
32598858	8	75	theme	moderate	1452:1459	arg1	exercise					1469:1476	moderate aerobic exercise	1452:1476	moderate aerobic exercise	1452:1476	However, 12 weeks of moderate aerobic exercise partially prevented these decrements in contractile properties.
32598858	3	76	theme	Twelve-week-old	497:511	arg1	n					519:519	n = 30	519:524	n = 30	519:524	Twelve-week-old rats (n = 30) were organized into either a chow diet or a high-fat, high-sucrose (HFHS) diet group.
32598858	3	76	theme	Twelve-week-old	497:511	arg1	rats					513:516	Twelve-week-old rats	497:516	Twelve-week-old rats (n = 30)	497:525	Twelve-week-old rats (n = 30) were organized into either a chow diet or a high-fat, high-sucrose (HFHS) diet group.
32598858	3	77	theme	high-fat	571:578	arg1	diet					601:604	a high-fat, high-sucrose (HFHS) diet	569:604	diet	601:604	Twelve-week-old rats (n = 30) were organized into either a chow diet or a high-fat, high-sucrose (HFHS) diet group.
32598858	7	78	theme	greater	1183:1189	arg1	mass					1191:1194	significantly greater mass	1169:1194	significantly greater mass (p < 0.03)	1169:1205	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	7	78	theme	greater	1183:1189	arg1	p <					1197:1199	p < 0.03	1197:1204	p < 0.03	1197:1204	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	7	78	theme	greater	1183:1189	arg1	velocity					1248:1255	significantly slower maximum shortening velocity	1208:1255	significantly slower maximum shortening velocity (p = 0.001)	1208:1267	Hearts from rats in the HFHS+Sed group had significantly greater mass (p < 0.03), significantly slower maximum shortening velocity (p = 0.001), and tended to have lower calcium sensitivity (p = 0.077) and a lower proportion of α-myosin heavy chain composition (p = 0.074) than the sedentary chow animals.
32598858	8	79	theme	exercise	1469:1476	arg1	12 weeks					1440:1447	12 weeks	1440:1447	12 weeks of moderate aerobic exercise	1440:1476	However, 12 weeks of moderate aerobic exercise partially prevented these decrements in contractile properties.
32598858	5	80	used	used	963:966	arg2	testing					951:957	skinned cardiac fibre bundle testing	922:957	skinned cardiac fibre bundle testing	922:957	Following 24 weeks of diet intervention, with 12 weeks of aerobic exercise (25 m/min, 30 min/day, 5 days/week) for the HFHS+Ex group, skinned cardiac fibre bundle testing was used to evaluate cardiac contractile properties.
32598858	10	81	theme	aerobic	1785:1791	arg1	regime					1802:1807	a 12-week aerobic exercise regime	1775:1807	a 12-week aerobic exercise regime	1775:1807	Obesity-induced impairment of contractile properties of the heart were partially prevented by a 12-week aerobic exercise regime.
32598858	4	82	theme	exercise	734:741	arg1	HFHS+Ex					744:750	HFHS+Ex	744:750	HFHS+Ex	744:750	Following 12 weeks of obesity induction the HFHS group animals were stratified and grouped into sedentary (HFHS+Sed) and exercise (HFHS+Ex) groups for an additional 12 weeks.
32598858	4	82	theme	exercise	734:741	arg1	groups					753:758	sedentary (HFHS+Sed) and exercise (HFHS+Ex) groups	709:758	groups	753:758	Following 12 weeks of obesity induction the HFHS group animals were stratified and grouped into sedentary (HFHS+Sed) and exercise (HFHS+Ex) groups for an additional 12 weeks.
32598858	3	83	dep	diet	601:604	arg1	high-sucrose					581:592	a high-fat, high-sucrose (HFHS) diet	569:604	high-sucrose	581:592	Twelve-week-old rats (n = 30) were organized into either a chow diet or a high-fat, high-sucrose (HFHS) diet group.
32598858	5	84	theme	cardiac	930:936	arg1	testing					951:957	skinned cardiac fibre bundle testing	922:957	skinned cardiac fibre bundle testing	922:957	Following 24 weeks of diet intervention, with 12 weeks of aerobic exercise (25 m/min, 30 min/day, 5 days/week) for the HFHS+Ex group, skinned cardiac fibre bundle testing was used to evaluate cardiac contractile properties.
32598858	3	85	theme	=	521:521	arg1	n					519:519	n = 30	519:524	n = 30	519:524	Twelve-week-old rats (n = 30) were organized into either a chow diet or a high-fat, high-sucrose (HFHS) diet group.
32598858	3	85	theme	=	521:521	arg1	rats					513:516	Twelve-week-old rats	497:516	Twelve-week-old rats (n = 30)	497:525	Twelve-week-old rats (n = 30) were organized into either a chow diet or a high-fat, high-sucrose (HFHS) diet group.
34694986	9	0	theme	name	1448:1451	arg1	sp					1482:1483	the name Pontibacter cellulosilyticus sp	1444:1483	the name Pontibacter cellulosilyticus sp	1444:1483	In accordance with the results of phenotypic, chemotaxonomic and phylogenetic data, strain SD6T represents a novel species of the genus Pontibacter, for which the name Pontibacter cellulosilyticus sp.
34694986	9	1	theme	genus	1415:1419	arg1	Pontibacter					1421:1431	the genus Pontibacter	1411:1431	the genus Pontibacter	1411:1431	In accordance with the results of phenotypic, chemotaxonomic and phylogenetic data, strain SD6T represents a novel species of the genus Pontibacter, for which the name Pontibacter cellulosilyticus sp.
34694986	7	2	theme	72.8-79.8 	1199:1208	arg1	values					1139:1144	The average nucleotide identity and in silico DNA-DNA hybridization values	1071:1144	The average nucleotide identity and in silico DNA-DNA hybridization values of strain SD6T with its closely related strains	1071:1192	The average nucleotide identity and in silico DNA-DNA hybridization values of strain SD6T with its closely related strains were 72.8-79.8 % and 19.2-22.6 %, respectively.
34694986	7	2	theme	72.8-79.8 	1199:1208	arg1	%					1225:1225	72.8-79.8 % and 19.2-22.6 %	1199:1225	72.8-79.8 % and 19.2-22.6 %	1199:1225	The average nucleotide identity and in silico DNA-DNA hybridization values of strain SD6T with its closely related strains were 72.8-79.8 % and 19.2-22.6 %, respectively.
34694986	4	3	theme	16S	419:421	arg1	rRNA					423:426	16S rRNA	419:426	16S rRNA gene sequence analysis	419:449	Moreover, 16S rRNA gene sequence analysis indicated that strain SD6T was a member of the genus Pontibacter, sharing similarities to Pontibacter aydingkolensis XAAS-1T (98.0 %), Pontibacter amylolyticus 9-2T (97.3 %), Pontibacter korlensis X14-1T (97.2 %) and Pontibacter soli HYL7-26T (96.8 %).
34694986	3	4	dep	optimum	344:350	arg1	pH					353:354	pH 6.0-7.0	353:362	pH 6.0-7.0	353:362	Cells of SD6T grew at 10-42 °C (optimum, 30 °C), pH 5.0-9.0 (optimum, pH 6.0-7.0) and at 0-8.0 % (w/v) NaCl (optimum, 0-3 %).
34694986	7	5	theme	average	1075:1081	arg1	identity					1094:1101	average nucleotide identity	1075:1101	average nucleotide identity	1075:1101	The average nucleotide identity and in silico DNA-DNA hybridization values of strain SD6T with its closely related strains were 72.8-79.8 % and 19.2-22.6 %, respectively.
34694986	2	6	theme	Gram-stain-negative	118:136	arg1	bacterium					172:180	A Gram-stain-negative, rod-shaped, non-motile, red-pink bacterium	116:180	A Gram-stain-negative, rod-shaped, non-motile, red-pink bacterium designated SD6T	116:196	A Gram-stain-negative, rod-shaped, non-motile, red-pink bacterium designated SD6T was isolated from coastal marine water at Sadong Beach, Ulleung Island, South Korea.
34694986	9	7	theme	cellulosilyticus	1465:1480	arg1	sp					1482:1483	the name Pontibacter cellulosilyticus sp	1444:1483	the name Pontibacter cellulosilyticus sp	1444:1483	In accordance with the results of phenotypic, chemotaxonomic and phylogenetic data, strain SD6T represents a novel species of the genus Pontibacter, for which the name Pontibacter cellulosilyticus sp.
34694986	7	8	theme	%	1209:1209	arg1	values					1139:1144	The average nucleotide identity and in silico DNA-DNA hybridization values	1071:1144	The average nucleotide identity and in silico DNA-DNA hybridization values of strain SD6T with its closely related strains	1071:1192	The average nucleotide identity and in silico DNA-DNA hybridization values of strain SD6T with its closely related strains were 72.8-79.8 % and 19.2-22.6 %, respectively.
34694986	7	8	theme	%	1209:1209	arg1	%					1225:1225	72.8-79.8 % and 19.2-22.6 %	1199:1225	72.8-79.8 % and 19.2-22.6 %	1199:1225	The average nucleotide identity and in silico DNA-DNA hybridization values of strain SD6T with its closely related strains were 72.8-79.8 % and 19.2-22.6 %, respectively.
34694986	1	9	theme	cellulose-hydrolysing	55:75	arg1	bacterium					77:85	a carboxymethyl cellulose-hydrolysing bacterium	39:85	a carboxymethyl cellulose-hydrolysing bacterium isolated from coastal water	39:113	nov., a carboxymethyl cellulose-hydrolysing bacterium isolated from coastal water.
34694986	1	9	theme	cellulose-hydrolysing	55:75	arg1	nov.					33:36	nov.	33:36	nov.	33:36	nov., a carboxymethyl cellulose-hydrolysing bacterium isolated from coastal water.
34694986	8	10	theme	G+C	1258:1260	arg1	%					1282:1282	45.4 mol%	1274:1282	45.4 mol%	1274:1282	The genomic DNA G+C content was 45.4 mol%.
34694986	8	10	theme	G+C	1258:1260	arg1	content					1262:1268	The genomic DNA G+C content	1242:1268	The genomic DNA G+C content	1242:1268	The genomic DNA G+C content was 45.4 mol%.
34694986	5	11	dep	 0	775:776	arg1	anteiso-C17 					811:822	comprising anteiso-C17 	800:822	comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I	800:848	The predominant fatty acids of strain SD6T were identified as iso-C15 : 0 and summed feature 4 (comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I) and the sole respiratory quinone was identified as MK-7 (menaquinone 7).
34694986	5	12	theme	comprising	800:809	arg1	anteiso-C17 					811:822	comprising anteiso-C17 	800:822	comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I	800:848	The predominant fatty acids of strain SD6T were identified as iso-C15 : 0 and summed feature 4 (comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I) and the sole respiratory quinone was identified as MK-7 (menaquinone 7).
34694986	7	13	theme	in	1107:1108	arg1	hybridization					1125:1137	in silico DNA-DNA hybridization	1107:1137	in silico DNA-DNA hybridization	1107:1137	The average nucleotide identity and in silico DNA-DNA hybridization values of strain SD6T with its closely related strains were 72.8-79.8 % and 19.2-22.6 %, respectively.
34694986	6	14	theme	polar	930:934	arg1	lipids					936:941	Major polar lipids	924:941	Major polar lipids	924:941	Major polar lipids included phosphatidylethanolamine, one unidentified phosphoglycolipid, two unidentified glycolipids and one unidentified lipid.
34694986	7	15	theme	strain	1149:1154	arg1	SD6T					1156:1159	strain SD6T	1149:1159	strain SD6T	1149:1159	The average nucleotide identity and in silico DNA-DNA hybridization values of strain SD6T with its closely related strains were 72.8-79.8 % and 19.2-22.6 %, respectively.
34694986	6	16	theme	unidentified	982:993	arg1	phosphoglycolipid					995:1011	one unidentified phosphoglycolipid	978:1011	one unidentified phosphoglycolipid	978:1011	Major polar lipids included phosphatidylethanolamine, one unidentified phosphoglycolipid, two unidentified glycolipids and one unidentified lipid.
34694986	5	17	theme	predominant	708:718	arg1	iso-C15 					766:773	iso-C15 	766:773	iso-C15 	766:773	The predominant fatty acids of strain SD6T were identified as iso-C15 : 0 and summed feature 4 (comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I) and the sole respiratory quinone was identified as MK-7 (menaquinone 7).
34694986	5	17	theme	predominant	708:718	arg1	acids					726:730	The predominant fatty acids	704:730	The predominant fatty acids of strain SD6T	704:745	The predominant fatty acids of strain SD6T were identified as iso-C15 : 0 and summed feature 4 (comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I) and the sole respiratory quinone was identified as MK-7 (menaquinone 7).
34694986	11	18	theme	114313T=JCM	1547:1557	arg1	31022T					1559:1564	=KACC 21543T=NBRC 114313T=JCM 31022T	1529:1564	=KACC 21543T=NBRC 114313T=JCM 31022T	1529:1564	The type strain is SD6T (=KACC 21543T=NBRC 114313T=JCM 31022T).
34694986	11	18	theme	114313T=JCM	1547:1557	arg1	SD6T					1523:1526	SD6T	1523:1526	SD6T (=KACC 21543T=NBRC 114313T=JCM 31022T)	1523:1565	The type strain is SD6T (=KACC 21543T=NBRC 114313T=JCM 31022T).
34694986	7	19	dep	in	1107:1108	arg1	silico					1110:1115	silico	1110:1115	silico	1110:1115	The average nucleotide identity and in silico DNA-DNA hybridization values of strain SD6T with its closely related strains were 72.8-79.8 % and 19.2-22.6 %, respectively.
34694986	3	20	theme	w/v	381:383	arg1	NaCl					386:389	0-8.0 % (w/v) NaCl	372:389	0-8.0 % (w/v) NaCl (optimum, 0-3 %)	372:406	Cells of SD6T grew at 10-42 °C (optimum, 30 °C), pH 5.0-9.0 (optimum, pH 6.0-7.0) and at 0-8.0 % (w/v) NaCl (optimum, 0-3 %).
34694986	3	20	theme	w/v	381:383	arg1	optimum					392:398	optimum	392:398	optimum	392:398	Cells of SD6T grew at 10-42 °C (optimum, 30 °C), pH 5.0-9.0 (optimum, pH 6.0-7.0) and at 0-8.0 % (w/v) NaCl (optimum, 0-3 %).
34694986	0	21	theme	cellulosilyticus	12:27	arg1	sp					29:30	Pontibacter cellulosilyticus sp	0:30	Pontibacter cellulosilyticus sp.	0:31	Pontibacter cellulosilyticus sp.
34694986	4	22	theme	strain	466:471	arg1	member					484:489	a member	482:489	a member of the genus Pontibacter	482:514	Moreover, 16S rRNA gene sequence analysis indicated that strain SD6T was a member of the genus Pontibacter, sharing similarities to Pontibacter aydingkolensis XAAS-1T (98.0 %), Pontibacter amylolyticus 9-2T (97.3 %), Pontibacter korlensis X14-1T (97.2 %) and Pontibacter soli HYL7-26T (96.8 %).
34694986	4	22	theme	strain	466:471	arg1	SD6T					473:476	strain SD6T	466:476	strain SD6T	466:476	Moreover, 16S rRNA gene sequence analysis indicated that strain SD6T was a member of the genus Pontibacter, sharing similarities to Pontibacter aydingkolensis XAAS-1T (98.0 %), Pontibacter amylolyticus 9-2T (97.3 %), Pontibacter korlensis X14-1T (97.2 %) and Pontibacter soli HYL7-26T (96.8 %).
34694986	9	23	theme	Pontibacter	1453:1463	arg1	sp					1482:1483	the name Pontibacter cellulosilyticus sp	1444:1483	the name Pontibacter cellulosilyticus sp	1444:1483	In accordance with the results of phenotypic, chemotaxonomic and phylogenetic data, strain SD6T represents a novel species of the genus Pontibacter, for which the name Pontibacter cellulosilyticus sp.
34694986	7	24	theme	identity	1094:1101	arg1	values					1139:1144	The average nucleotide identity and in silico DNA-DNA hybridization values	1071:1144	The average nucleotide identity and in silico DNA-DNA hybridization values of strain SD6T with its closely related strains	1071:1192	The average nucleotide identity and in silico DNA-DNA hybridization values of strain SD6T with its closely related strains were 72.8-79.8 % and 19.2-22.6 %, respectively.
34694986	7	24	theme	identity	1094:1101	arg1	%					1225:1225	72.8-79.8 % and 19.2-22.6 %	1199:1225	72.8-79.8 % and 19.2-22.6 %	1199:1225	The average nucleotide identity and in silico DNA-DNA hybridization values of strain SD6T with its closely related strains were 72.8-79.8 % and 19.2-22.6 %, respectively.
34694986	1	25	theme	coastal	101:107	arg1	water					109:113	coastal water	101:113	coastal water	101:113	nov., a carboxymethyl cellulose-hydrolysing bacterium isolated from coastal water.
34694986	7	26	theme	SD6T	1156:1159	arg1	values					1139:1144	The average nucleotide identity and in silico DNA-DNA hybridization values	1071:1144	The average nucleotide identity and in silico DNA-DNA hybridization values of strain SD6T with its closely related strains	1071:1192	The average nucleotide identity and in silico DNA-DNA hybridization values of strain SD6T with its closely related strains were 72.8-79.8 % and 19.2-22.6 %, respectively.
34694986	7	26	theme	SD6T	1156:1159	arg1	%					1225:1225	72.8-79.8 % and 19.2-22.6 %	1199:1225	72.8-79.8 % and 19.2-22.6 %	1199:1225	The average nucleotide identity and in silico DNA-DNA hybridization values of strain SD6T with its closely related strains were 72.8-79.8 % and 19.2-22.6 %, respectively.
34694986	0	27	theme	Pontibacter	0:10	arg1	sp					29:30	Pontibacter cellulosilyticus sp	0:30	Pontibacter cellulosilyticus sp.	0:31	Pontibacter cellulosilyticus sp.
34694986	11	28	theme	=KACC	1529:1533	arg1	31022T					1559:1564	=KACC 21543T=NBRC 114313T=JCM 31022T	1529:1564	=KACC 21543T=NBRC 114313T=JCM 31022T	1529:1564	The type strain is SD6T (=KACC 21543T=NBRC 114313T=JCM 31022T).
34694986	11	28	theme	=KACC	1529:1533	arg1	SD6T					1523:1526	SD6T	1523:1526	SD6T (=KACC 21543T=NBRC 114313T=JCM 31022T)	1523:1565	The type strain is SD6T (=KACC 21543T=NBRC 114313T=JCM 31022T).
34694986	5	29	theme	summed	782:787	arg1	feature					789:795	summed feature 4	782:797	summed feature 4	782:797	The predominant fatty acids of strain SD6T were identified as iso-C15 : 0 and summed feature 4 (comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I) and the sole respiratory quinone was identified as MK-7 (menaquinone 7).
34694986	5	29	theme	summed	782:787	arg1	MK-7					902:905	MK-7	902:905	MK-7 (menaquinone 7)	902:921	The predominant fatty acids of strain SD6T were identified as iso-C15 : 0 and summed feature 4 (comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I) and the sole respiratory quinone was identified as MK-7 (menaquinone 7).
34694986	5	29	theme	summed	782:787	arg1	quinone					876:882	the sole respiratory quinone	855:882	the sole respiratory quinone	855:882	The predominant fatty acids of strain SD6T were identified as iso-C15 : 0 and summed feature 4 (comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I) and the sole respiratory quinone was identified as MK-7 (menaquinone 7).
34694986	5	29	theme	summed	782:787	arg1	 0					775:776	 0	775:776	 0	775:776	The predominant fatty acids of strain SD6T were identified as iso-C15 : 0 and summed feature 4 (comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I) and the sole respiratory quinone was identified as MK-7 (menaquinone 7).
34694986	7	30	with	values	1139:1144	arg1	strains					1186:1192	its closely related strains	1166:1192	its closely related strains	1166:1192	The average nucleotide identity and in silico DNA-DNA hybridization values of strain SD6T with its closely related strains were 72.8-79.8 % and 19.2-22.6 %, respectively.
34694986	3	31	theme	%	378:378	arg1	NaCl					386:389	0-8.0 % (w/v) NaCl	372:389	0-8.0 % (w/v) NaCl (optimum, 0-3 %)	372:406	Cells of SD6T grew at 10-42 °C (optimum, 30 °C), pH 5.0-9.0 (optimum, pH 6.0-7.0) and at 0-8.0 % (w/v) NaCl (optimum, 0-3 %).
34694986	3	31	theme	%	378:378	arg1	optimum					392:398	optimum	392:398	optimum	392:398	Cells of SD6T grew at 10-42 °C (optimum, 30 °C), pH 5.0-9.0 (optimum, pH 6.0-7.0) and at 0-8.0 % (w/v) NaCl (optimum, 0-3 %).
34694986	7	32	theme	related	1178:1184	arg1	strains					1186:1192	its closely related strains	1166:1192	its closely related strains	1166:1192	The average nucleotide identity and in silico DNA-DNA hybridization values of strain SD6T with its closely related strains were 72.8-79.8 % and 19.2-22.6 %, respectively.
34694986	9	33	theme	Pontibacter	1421:1431	arg1	species					1400:1406	a novel species	1392:1406	a novel species	1392:1406	In accordance with the results of phenotypic, chemotaxonomic and phylogenetic data, strain SD6T represents a novel species of the genus Pontibacter, for which the name Pontibacter cellulosilyticus sp.
34694986	9	34	theme	novel	1394:1398	arg1	species					1400:1406	a novel species	1392:1406	a novel species	1392:1406	In accordance with the results of phenotypic, chemotaxonomic and phylogenetic data, strain SD6T represents a novel species of the genus Pontibacter, for which the name Pontibacter cellulosilyticus sp.
34694986	3	35	dep	optimum	315:321	arg1	30 °C					324:328	30 °C	324:328	30 °C	324:328	Cells of SD6T grew at 10-42 °C (optimum, 30 °C), pH 5.0-9.0 (optimum, pH 6.0-7.0) and at 0-8.0 % (w/v) NaCl (optimum, 0-3 %).
34694986	11	36	theme	type	1508:1511	arg1	SD6T					1523:1526	SD6T	1523:1526	SD6T (=KACC 21543T=NBRC 114313T=JCM 31022T)	1523:1565	The type strain is SD6T (=KACC 21543T=NBRC 114313T=JCM 31022T).
34694986	11	36	theme	type	1508:1511	arg1	strain					1513:1518	The type strain	1504:1518	The type strain	1504:1518	The type strain is SD6T (=KACC 21543T=NBRC 114313T=JCM 31022T).
34694986	9	37	theme	phylogenetic	1350:1361	arg1	data					1363:1366	phenotypic, chemotaxonomic and phylogenetic data	1319:1366	phenotypic, chemotaxonomic and phylogenetic data	1319:1366	In accordance with the results of phenotypic, chemotaxonomic and phylogenetic data, strain SD6T represents a novel species of the genus Pontibacter, for which the name Pontibacter cellulosilyticus sp.
34694986	2	38	attach	isolated	202:209	arg2	bacterium					172:180	A Gram-stain-negative, rod-shaped, non-motile, red-pink bacterium	116:180	A Gram-stain-negative, rod-shaped, non-motile, red-pink bacterium designated SD6T	116:196	A Gram-stain-negative, rod-shaped, non-motile, red-pink bacterium designated SD6T was isolated from coastal marine water at Sadong Beach, Ulleung Island, South Korea.
34694986	2	38	attach	isolated	202:209	arg1	water					231:235	coastal marine water	216:235	coastal marine water at Sadong Beach, Ulleung Island, South Korea	216:280	A Gram-stain-negative, rod-shaped, non-motile, red-pink bacterium designated SD6T was isolated from coastal marine water at Sadong Beach, Ulleung Island, South Korea.
34694986	1	39	attach	isolated	87:94	arg1	water					109:113	coastal water	101:113	coastal water	101:113	nov., a carboxymethyl cellulose-hydrolysing bacterium isolated from coastal water.
34694986	1	39	attach	isolated	87:94	arg2	nov.					33:36	nov.	33:36	nov.	33:36	nov., a carboxymethyl cellulose-hydrolysing bacterium isolated from coastal water.
34694986	1	39	attach	isolated	87:94	arg2	bacterium					77:85	a carboxymethyl cellulose-hydrolysing bacterium	39:85	a carboxymethyl cellulose-hydrolysing bacterium isolated from coastal water	39:113	nov., a carboxymethyl cellulose-hydrolysing bacterium isolated from coastal water.
34694986	7	40	theme	nucleotide	1083:1092	arg1	identity					1094:1101	average nucleotide identity	1075:1101	average nucleotide identity	1075:1101	The average nucleotide identity and in silico DNA-DNA hybridization values of strain SD6T with its closely related strains were 72.8-79.8 % and 19.2-22.6 %, respectively.
34694986	7	41	theme	hybridization	1125:1137	arg1	values					1139:1144	The average nucleotide identity and in silico DNA-DNA hybridization values	1071:1144	The average nucleotide identity and in silico DNA-DNA hybridization values of strain SD6T with its closely related strains	1071:1192	The average nucleotide identity and in silico DNA-DNA hybridization values of strain SD6T with its closely related strains were 72.8-79.8 % and 19.2-22.6 %, respectively.
34694986	7	41	theme	hybridization	1125:1137	arg1	%					1225:1225	72.8-79.8 % and 19.2-22.6 %	1199:1225	72.8-79.8 % and 19.2-22.6 %	1199:1225	The average nucleotide identity and in silico DNA-DNA hybridization values of strain SD6T with its closely related strains were 72.8-79.8 % and 19.2-22.6 %, respectively.
34694986	4	42	theme	gene	428:431	arg1	analysis					442:449	16S rRNA gene sequence analysis	419:449	16S rRNA gene sequence analysis	419:449	Moreover, 16S rRNA gene sequence analysis indicated that strain SD6T was a member of the genus Pontibacter, sharing similarities to Pontibacter aydingkolensis XAAS-1T (98.0 %), Pontibacter amylolyticus 9-2T (97.3 %), Pontibacter korlensis X14-1T (97.2 %) and Pontibacter soli HYL7-26T (96.8 %).
34694986	5	43	theme	 1	845:846	arg1	I					848:848	 1 I	845:848	comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I	800:848	The predominant fatty acids of strain SD6T were identified as iso-C15 : 0 and summed feature 4 (comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I) and the sole respiratory quinone was identified as MK-7 (menaquinone 7).
34694986	4	44	theme	sequence	433:440	arg1	analysis					442:449	16S rRNA gene sequence analysis	419:449	16S rRNA gene sequence analysis	419:449	Moreover, 16S rRNA gene sequence analysis indicated that strain SD6T was a member of the genus Pontibacter, sharing similarities to Pontibacter aydingkolensis XAAS-1T (98.0 %), Pontibacter amylolyticus 9-2T (97.3 %), Pontibacter korlensis X14-1T (97.2 %) and Pontibacter soli HYL7-26T (96.8 %).
34694986	4	45	theme	rRNA	423:426	arg1	analysis					442:449	16S rRNA gene sequence analysis	419:449	16S rRNA gene sequence analysis	419:449	Moreover, 16S rRNA gene sequence analysis indicated that strain SD6T was a member of the genus Pontibacter, sharing similarities to Pontibacter aydingkolensis XAAS-1T (98.0 %), Pontibacter amylolyticus 9-2T (97.3 %), Pontibacter korlensis X14-1T (97.2 %) and Pontibacter soli HYL7-26T (96.8 %).
34694986	8	46	theme	mol	1279:1281	arg1	%					1282:1282	45.4 mol%	1274:1282	45.4 mol%	1274:1282	The genomic DNA G+C content was 45.4 mol%.
34694986	8	46	theme	mol	1279:1281	arg1	content					1262:1268	The genomic DNA G+C content	1242:1268	The genomic DNA G+C content	1242:1268	The genomic DNA G+C content was 45.4 mol%.
34694986	6	47	theme	unidentified	1051:1062	arg1	lipid					1064:1068	one unidentified lipid	1047:1068	one unidentified lipid	1047:1068	Major polar lipids included phosphatidylethanolamine, one unidentified phosphoglycolipid, two unidentified glycolipids and one unidentified lipid.
34694986	4	48	theme	Pontibacter	504:514	arg1	member					484:489	a member	482:489	a member of the genus Pontibacter	482:514	Moreover, 16S rRNA gene sequence analysis indicated that strain SD6T was a member of the genus Pontibacter, sharing similarities to Pontibacter aydingkolensis XAAS-1T (98.0 %), Pontibacter amylolyticus 9-2T (97.3 %), Pontibacter korlensis X14-1T (97.2 %) and Pontibacter soli HYL7-26T (96.8 %).
34694986	4	48	theme	Pontibacter	504:514	arg1	SD6T					473:476	strain SD6T	466:476	strain SD6T	466:476	Moreover, 16S rRNA gene sequence analysis indicated that strain SD6T was a member of the genus Pontibacter, sharing similarities to Pontibacter aydingkolensis XAAS-1T (98.0 %), Pontibacter amylolyticus 9-2T (97.3 %), Pontibacter korlensis X14-1T (97.2 %) and Pontibacter soli HYL7-26T (96.8 %).
34694986	5	49	dep	anteiso-C17 	811:822	arg1	iso-C17 					836:843	iso-C17 	836:843	iso-C17 	836:843	The predominant fatty acids of strain SD6T were identified as iso-C15 : 0 and summed feature 4 (comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I) and the sole respiratory quinone was identified as MK-7 (menaquinone 7).
34694986	5	49	dep	anteiso-C17 	811:822	arg1	I					848:848	 1 I	845:848	comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I	800:848	The predominant fatty acids of strain SD6T were identified as iso-C15 : 0 and summed feature 4 (comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I) and the sole respiratory quinone was identified as MK-7 (menaquinone 7).
34694986	5	49	dep	anteiso-C17 	811:822	arg1	B					827:827	 1 B	824:827	 1 B	824:827	The predominant fatty acids of strain SD6T were identified as iso-C15 : 0 and summed feature 4 (comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I) and the sole respiratory quinone was identified as MK-7 (menaquinone 7).
34694986	9	50	theme	phenotypic	1319:1328	arg1	data					1363:1366	phenotypic, chemotaxonomic and phylogenetic data	1319:1366	phenotypic, chemotaxonomic and phylogenetic data	1319:1366	In accordance with the results of phenotypic, chemotaxonomic and phylogenetic data, strain SD6T represents a novel species of the genus Pontibacter, for which the name Pontibacter cellulosilyticus sp.
34694986	9	51	theme	data	1363:1366	arg1	results					1308:1314	the results	1304:1314	the results of phenotypic, chemotaxonomic and phylogenetic data	1304:1366	In accordance with the results of phenotypic, chemotaxonomic and phylogenetic data, strain SD6T represents a novel species of the genus Pontibacter, for which the name Pontibacter cellulosilyticus sp.
34694986	7	52	theme	 and	1210:1213	arg1	values					1139:1144	The average nucleotide identity and in silico DNA-DNA hybridization values	1071:1144	The average nucleotide identity and in silico DNA-DNA hybridization values of strain SD6T with its closely related strains	1071:1192	The average nucleotide identity and in silico DNA-DNA hybridization values of strain SD6T with its closely related strains were 72.8-79.8 % and 19.2-22.6 %, respectively.
34694986	7	52	theme	 and	1210:1213	arg1	%					1225:1225	72.8-79.8 % and 19.2-22.6 %	1199:1225	72.8-79.8 % and 19.2-22.6 %	1199:1225	The average nucleotide identity and in silico DNA-DNA hybridization values of strain SD6T with its closely related strains were 72.8-79.8 % and 19.2-22.6 %, respectively.
34694986	6	53	theme	Major	924:928	arg1	lipids					936:941	Major polar lipids	924:941	Major polar lipids	924:941	Major polar lipids included phosphatidylethanolamine, one unidentified phosphoglycolipid, two unidentified glycolipids and one unidentified lipid.
34694986	8	54	theme	DNA	1254:1256	arg1	%					1282:1282	45.4 mol%	1274:1282	45.4 mol%	1274:1282	The genomic DNA G+C content was 45.4 mol%.
34694986	8	54	theme	DNA	1254:1256	arg1	content					1262:1268	The genomic DNA G+C content	1242:1268	The genomic DNA G+C content	1242:1268	The genomic DNA G+C content was 45.4 mol%.
34694986	1	55	theme	carboxymethyl	41:53	arg1	bacterium					77:85	a carboxymethyl cellulose-hydrolysing bacterium	39:85	a carboxymethyl cellulose-hydrolysing bacterium isolated from coastal water	39:113	nov., a carboxymethyl cellulose-hydrolysing bacterium isolated from coastal water.
34694986	1	55	theme	carboxymethyl	41:53	arg1	nov.					33:36	nov.	33:36	nov.	33:36	nov., a carboxymethyl cellulose-hydrolysing bacterium isolated from coastal water.
34694986	3	56	theme	SD6T	292:295	arg1	Cells					283:287	Cells	283:287	Cells of SD6T	283:295	Cells of SD6T grew at 10-42 °C (optimum, 30 °C), pH 5.0-9.0 (optimum, pH 6.0-7.0) and at 0-8.0 % (w/v) NaCl (optimum, 0-3 %).
34694986	2	57	theme	marine	224:229	arg1	water					231:235	coastal marine water	216:235	coastal marine water at Sadong Beach, Ulleung Island, South Korea	216:280	A Gram-stain-negative, rod-shaped, non-motile, red-pink bacterium designated SD6T was isolated from coastal marine water at Sadong Beach, Ulleung Island, South Korea.
34694986	5	58	theme	 1	824:825	arg1	B					827:827	 1 B	824:827	 1 B	824:827	The predominant fatty acids of strain SD6T were identified as iso-C15 : 0 and summed feature 4 (comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I) and the sole respiratory quinone was identified as MK-7 (menaquinone 7).
34694986	9	59	theme	chemotaxonomic	1331:1344	arg1	data					1363:1366	phenotypic, chemotaxonomic and phylogenetic data	1319:1366	phenotypic, chemotaxonomic and phylogenetic data	1319:1366	In accordance with the results of phenotypic, chemotaxonomic and phylogenetic data, strain SD6T represents a novel species of the genus Pontibacter, for which the name Pontibacter cellulosilyticus sp.
34694986	2	60	from	Korea	276:280	arg1	water					231:235	coastal marine water	216:235	coastal marine water at Sadong Beach, Ulleung Island, South Korea	216:280	A Gram-stain-negative, rod-shaped, non-motile, red-pink bacterium designated SD6T was isolated from coastal marine water at Sadong Beach, Ulleung Island, South Korea.
34694986	4	61	theme	genus	498:502	arg1	Pontibacter					504:514	the genus Pontibacter	494:514	the genus Pontibacter	494:514	Moreover, 16S rRNA gene sequence analysis indicated that strain SD6T was a member of the genus Pontibacter, sharing similarities to Pontibacter aydingkolensis XAAS-1T (98.0 %), Pontibacter amylolyticus 9-2T (97.3 %), Pontibacter korlensis X14-1T (97.2 %) and Pontibacter soli HYL7-26T (96.8 %).
34694986	2	62	theme	coastal	216:222	arg1	water					231:235	coastal marine water	216:235	coastal marine water at Sadong Beach, Ulleung Island, South Korea	216:280	A Gram-stain-negative, rod-shaped, non-motile, red-pink bacterium designated SD6T was isolated from coastal marine water at Sadong Beach, Ulleung Island, South Korea.
34694986	4	63	dep	Pontibacter	586:596	arg1	9-2T					611:614	9-2T	611:614	9-2T	611:614	Moreover, 16S rRNA gene sequence analysis indicated that strain SD6T was a member of the genus Pontibacter, sharing similarities to Pontibacter aydingkolensis XAAS-1T (98.0 %), Pontibacter amylolyticus 9-2T (97.3 %), Pontibacter korlensis X14-1T (97.2 %) and Pontibacter soli HYL7-26T (96.8 %).
34694986	4	63	dep	Pontibacter	586:596	arg1	%					622:622	97.3 %	617:622	97.3 %	617:622	Moreover, 16S rRNA gene sequence analysis indicated that strain SD6T was a member of the genus Pontibacter, sharing similarities to Pontibacter aydingkolensis XAAS-1T (98.0 %), Pontibacter amylolyticus 9-2T (97.3 %), Pontibacter korlensis X14-1T (97.2 %) and Pontibacter soli HYL7-26T (96.8 %).
34694986	4	63	dep	Pontibacter	586:596	arg1	amylolyticus					598:609	Pontibacter amylolyticus 9-2T (97.3 %)	586:623	Pontibacter amylolyticus 9-2T (97.3 %)	586:623	Moreover, 16S rRNA gene sequence analysis indicated that strain SD6T was a member of the genus Pontibacter, sharing similarities to Pontibacter aydingkolensis XAAS-1T (98.0 %), Pontibacter amylolyticus 9-2T (97.3 %), Pontibacter korlensis X14-1T (97.2 %) and Pontibacter soli HYL7-26T (96.8 %).
34694986	5	64	theme	SD6T	742:745	arg1	iso-C15 					766:773	iso-C15 	766:773	iso-C15 	766:773	The predominant fatty acids of strain SD6T were identified as iso-C15 : 0 and summed feature 4 (comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I) and the sole respiratory quinone was identified as MK-7 (menaquinone 7).
34694986	5	64	theme	SD6T	742:745	arg1	acids					726:730	The predominant fatty acids	704:730	The predominant fatty acids of strain SD6T	704:745	The predominant fatty acids of strain SD6T were identified as iso-C15 : 0 and summed feature 4 (comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I) and the sole respiratory quinone was identified as MK-7 (menaquinone 7).
34694986	5	65	theme	sole	859:862	arg1	feature					789:795	summed feature 4	782:797	summed feature 4	782:797	The predominant fatty acids of strain SD6T were identified as iso-C15 : 0 and summed feature 4 (comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I) and the sole respiratory quinone was identified as MK-7 (menaquinone 7).
34694986	5	65	theme	sole	859:862	arg1	MK-7					902:905	MK-7	902:905	MK-7 (menaquinone 7)	902:921	The predominant fatty acids of strain SD6T were identified as iso-C15 : 0 and summed feature 4 (comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I) and the sole respiratory quinone was identified as MK-7 (menaquinone 7).
34694986	5	65	theme	sole	859:862	arg1	quinone					876:882	the sole respiratory quinone	855:882	the sole respiratory quinone	855:882	The predominant fatty acids of strain SD6T were identified as iso-C15 : 0 and summed feature 4 (comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I) and the sole respiratory quinone was identified as MK-7 (menaquinone 7).
34694986	5	65	theme	sole	859:862	arg1	 0					775:776	 0	775:776	 0	775:776	The predominant fatty acids of strain SD6T were identified as iso-C15 : 0 and summed feature 4 (comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I) and the sole respiratory quinone was identified as MK-7 (menaquinone 7).
34694986	8	66	theme	genomic	1246:1252	arg1	%					1282:1282	45.4 mol%	1274:1282	45.4 mol%	1274:1282	The genomic DNA G+C content was 45.4 mol%.
34694986	8	66	theme	genomic	1246:1252	arg1	content					1262:1268	The genomic DNA G+C content	1242:1268	The genomic DNA G+C content	1242:1268	The genomic DNA G+C content was 45.4 mol%.
34694986	5	67	theme	respiratory	864:874	arg1	feature					789:795	summed feature 4	782:797	summed feature 4	782:797	The predominant fatty acids of strain SD6T were identified as iso-C15 : 0 and summed feature 4 (comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I) and the sole respiratory quinone was identified as MK-7 (menaquinone 7).
34694986	5	67	theme	respiratory	864:874	arg1	MK-7					902:905	MK-7	902:905	MK-7 (menaquinone 7)	902:921	The predominant fatty acids of strain SD6T were identified as iso-C15 : 0 and summed feature 4 (comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I) and the sole respiratory quinone was identified as MK-7 (menaquinone 7).
34694986	5	67	theme	respiratory	864:874	arg1	quinone					876:882	the sole respiratory quinone	855:882	the sole respiratory quinone	855:882	The predominant fatty acids of strain SD6T were identified as iso-C15 : 0 and summed feature 4 (comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I) and the sole respiratory quinone was identified as MK-7 (menaquinone 7).
34694986	5	67	theme	respiratory	864:874	arg1	 0					775:776	 0	775:776	 0	775:776	The predominant fatty acids of strain SD6T were identified as iso-C15 : 0 and summed feature 4 (comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I) and the sole respiratory quinone was identified as MK-7 (menaquinone 7).
34694986	6	68	theme	unidentified	1018:1029	arg1	glycolipids					1031:1041	two unidentified glycolipids	1014:1041	two unidentified glycolipids	1014:1041	Major polar lipids included phosphatidylethanolamine, one unidentified phosphoglycolipid, two unidentified glycolipids and one unidentified lipid.
34694986	5	69	theme	strain	735:740	arg1	SD6T					742:745	strain SD6T	735:745	strain SD6T	735:745	The predominant fatty acids of strain SD6T were identified as iso-C15 : 0 and summed feature 4 (comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I) and the sole respiratory quinone was identified as MK-7 (menaquinone 7).
34694986	5	70	theme	fatty	720:724	arg1	iso-C15 					766:773	iso-C15 	766:773	iso-C15 	766:773	The predominant fatty acids of strain SD6T were identified as iso-C15 : 0 and summed feature 4 (comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I) and the sole respiratory quinone was identified as MK-7 (menaquinone 7).
34694986	5	70	theme	fatty	720:724	arg1	acids					726:730	The predominant fatty acids	704:730	The predominant fatty acids of strain SD6T	704:745	The predominant fatty acids of strain SD6T were identified as iso-C15 : 0 and summed feature 4 (comprising anteiso-C17 : 1 B and/or iso-C17 : 1 I) and the sole respiratory quinone was identified as MK-7 (menaquinone 7).
34694986	11	71	theme	21543T=NBRC	1535:1545	arg1	31022T					1559:1564	=KACC 21543T=NBRC 114313T=JCM 31022T	1529:1564	=KACC 21543T=NBRC 114313T=JCM 31022T	1529:1564	The type strain is SD6T (=KACC 21543T=NBRC 114313T=JCM 31022T).
34694986	11	71	theme	21543T=NBRC	1535:1545	arg1	SD6T					1523:1526	SD6T	1523:1526	SD6T (=KACC 21543T=NBRC 114313T=JCM 31022T)	1523:1565	The type strain is SD6T (=KACC 21543T=NBRC 114313T=JCM 31022T).
34694986	3	72	dep	optimum	392:398	arg1	%					405:405	0-3 %	401:405	0-3 %	401:405	Cells of SD6T grew at 10-42 °C (optimum, 30 °C), pH 5.0-9.0 (optimum, pH 6.0-7.0) and at 0-8.0 % (w/v) NaCl (optimum, 0-3 %).
34694986	9	73	theme	strain	1369:1374	arg1	SD6T					1376:1379	strain SD6T	1369:1379	strain SD6T	1369:1379	In accordance with the results of phenotypic, chemotaxonomic and phylogenetic data, strain SD6T represents a novel species of the genus Pontibacter, for which the name Pontibacter cellulosilyticus sp.
34694986	2	74	dep	Korea	276:280	arg1	South					270:274	South	270:274	South	270:274	A Gram-stain-negative, rod-shaped, non-motile, red-pink bacterium designated SD6T was isolated from coastal marine water at Sadong Beach, Ulleung Island, South Korea.
34694986	7	75	theme	DNA-DNA	1117:1123	arg1	hybridization					1125:1137	in silico DNA-DNA hybridization	1107:1137	in silico DNA-DNA hybridization	1107:1137	The average nucleotide identity and in silico DNA-DNA hybridization values of strain SD6T with its closely related strains were 72.8-79.8 % and 19.2-22.6 %, respectively.
34694986	2	76	dep	Gram-stain-negative	118:136	arg1	rod-shaped					139:148	rod-shaped	139:148	rod-shaped	139:148	A Gram-stain-negative, rod-shaped, non-motile, red-pink bacterium designated SD6T was isolated from coastal marine water at Sadong Beach, Ulleung Island, South Korea.
34694986	2	76	dep	Gram-stain-negative	118:136	arg1	red-pink					163:170	red-pink	163:170	red-pink	163:170	A Gram-stain-negative, rod-shaped, non-motile, red-pink bacterium designated SD6T was isolated from coastal marine water at Sadong Beach, Ulleung Island, South Korea.
34694986	2	76	dep	Gram-stain-negative	118:136	arg1	non-motile					151:160	non-motile	151:160	non-motile	151:160	A Gram-stain-negative, rod-shaped, non-motile, red-pink bacterium designated SD6T was isolated from coastal marine water at Sadong Beach, Ulleung Island, South Korea.
33044149	2	0	theme	novel	97:101	arg1	bacterium					189:197	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium	95:197	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium designated Q22T	95:213	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium designated Q22T was isolated from the rhizosphere soil of mangrove plant, Kandelia candel collected in Zhangzhou, Fujian province, China.
33044149	5	1	theme	nucleotide	519:528	arg1	identity					530:537	The average nucleotide identity	507:537	The average nucleotide identity	507:537	The average nucleotide identity, and in silico DNA-DNA hybridization values between strain Q22T and the reference strains were 79.7-88.9% and 22.6-37.4%, respectively.
33044149	5	2	theme	hybridization	562:574	arg1	values					576:581	The average nucleotide identity, and in silico DNA-DNA hybridization values	507:581	The average nucleotide identity, and in silico DNA-DNA hybridization values between strain Q22T and the reference strains	507:627	The average nucleotide identity, and in silico DNA-DNA hybridization values between strain Q22T and the reference strains were 79.7-88.9% and 22.6-37.4%, respectively.
33044149	2	3	attach	isolated	219:226	arg2	bacterium					189:197	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium	95:197	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium designated Q22T	95:213	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium designated Q22T was isolated from the rhizosphere soil of mangrove plant, Kandelia candel collected in Zhangzhou, Fujian province, China.
33044149	2	3	attach	isolated	219:226	arg1	soil					249:252	the rhizosphere soil	233:252	the rhizosphere soil of mangrove plant	233:270	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium designated Q22T was isolated from the rhizosphere soil of mangrove plant, Kandelia candel collected in Zhangzhou, Fujian province, China.
33044149	2	3	attach	isolated	219:226	arg1	candel					282:287	candel	282:287	candel	282:287	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium designated Q22T was isolated from the rhizosphere soil of mangrove plant, Kandelia candel collected in Zhangzhou, Fujian province, China.
33044149	9	4	theme	novel	1195:1199	arg1	species					1201:1207	a novel species	1193:1207	a novel species	1193:1207	The phylogenetic analysis and genotypic features, along with the phenotypic and chemotaxonomic characteristics, indicate that strain Q22T represents a novel species of the genus Agromyces, for which the name Agromyces kandeliae sp.
33044149	5	5	theme	identity	530:537	arg1	values					576:581	The average nucleotide identity, and in silico DNA-DNA hybridization values	507:581	The average nucleotide identity, and in silico DNA-DNA hybridization values between strain Q22T and the reference strains	507:627	The average nucleotide identity, and in silico DNA-DNA hybridization values between strain Q22T and the reference strains were 79.7-88.9% and 22.6-37.4%, respectively.
33044149	6	6	theme	isoprenoid	691:700	arg1	MK-12					714:718	MK-12	714:718	MK-12	714:718	The predominant isoprenoid quinone was MK-12 and the major fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0.
33044149	6	6	theme	isoprenoid	691:700	arg1	quinone					702:708	The predominant isoprenoid quinone	675:708	The predominant isoprenoid quinone	675:708	The predominant isoprenoid quinone was MK-12 and the major fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0.
33044149	3	7	theme	Strain	337:342	arg1	Q22T					344:347	Strain Q22T	337:347	Strain Q22T	337:347	Strain Q22T was able to grow at 10-40 °C (optimum 30 °C), pH 5.5-9.0 (optimum 7.0-8.0) and with 0-5.0% (w/v) NaCl (optimum 1.0 %).
33044149	8	8	theme	strain	934:939	arg1	Q22T					941:944	The strain Q22T	930:944	The strain Q22T	930:944	The strain Q22T contained 2,4-diaminobutyric acid, alanine acid, glutamic acid and glycine in the peptidoglycans.
33044149	6	9	theme	predominant	679:689	arg1	MK-12					714:718	MK-12	714:718	MK-12	714:718	The predominant isoprenoid quinone was MK-12 and the major fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0.
33044149	6	9	theme	predominant	679:689	arg1	quinone					702:708	The predominant isoprenoid quinone	675:708	The predominant isoprenoid quinone	675:708	The predominant isoprenoid quinone was MK-12 and the major fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0.
33044149	1	10	theme	rhizosphere	44:54	arg1	soil					56:59	rhizosphere soil	44:59	rhizosphere soil of Kandelia	44:71	nov., isolated from rhizosphere soil of Kandelia candel in a mangrove.
33044149	4	11	theme	DNA	480:482	arg1	content					488:494	The genomic DNA G+C content	468:494	The genomic DNA G+C content	468:494	The genomic DNA G+C content was 71.9%.
33044149	4	11	theme	DNA	480:482	arg1	%					504:504	71.9%	500:504	71.9%	500:504	The genomic DNA G+C content was 71.9%.
33044149	8	12	theme	2,4-diaminobutyric	956:973	arg1	acid					975:978	2,4-diaminobutyric acid	956:978	2,4-diaminobutyric acid	956:978	The strain Q22T contained 2,4-diaminobutyric acid, alanine acid, glutamic acid and glycine in the peptidoglycans.
33044149	7	13	theme	strain	821:826	arg1	Q22T					828:831	strain Q22T	821:831	strain Q22T	821:831	The major polar lipids of strain Q22T were diphosphatidylglycerol, phosphatidylglycerol, one glycolipid and three unidentified lipids.
33044149	11	14	theme	=MCCC	1319:1323	arg1	39961T					1340:1345	=MCCC 1K03340T= KCTC 39961T	1319:1345	=MCCC 1K03340T= KCTC 39961T	1319:1345	The type strain is Q22T (=MCCC 1K03340T= KCTC 39961T).
33044149	11	14	theme	=MCCC	1319:1323	arg1	Q22T					1313:1316	Q22T	1313:1316	Q22T (=MCCC 1K03340T= KCTC 39961T)	1313:1346	The type strain is Q22T (=MCCC 1K03340T= KCTC 39961T).
33044149	1	15	attach	isolated	30:37	arg2	nov.					24:27	nov.	24:27	nov.	24:27	nov., isolated from rhizosphere soil of Kandelia candel in a mangrove.
33044149	1	15	attach	isolated	30:37	arg1	soil					56:59	rhizosphere soil	44:59	rhizosphere soil of Kandelia	44:71	nov., isolated from rhizosphere soil of Kandelia candel in a mangrove.
33044149	5	16	theme	reference	611:619	arg1	strains					621:627	the reference strains	607:627	the reference strains	607:627	The average nucleotide identity, and in silico DNA-DNA hybridization values between strain Q22T and the reference strains were 79.7-88.9% and 22.6-37.4%, respectively.
33044149	2	17	theme	aerobic	125:131	arg1	bacterium					189:197	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium	95:197	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium designated Q22T	95:213	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium designated Q22T was isolated from the rhizosphere soil of mangrove plant, Kandelia candel collected in Zhangzhou, Fujian province, China.
33044149	7	18	theme	Q22T	828:831	arg1	lipids					811:816	The major polar lipids	795:816	The major polar lipids of strain Q22T	795:831	The major polar lipids of strain Q22T were diphosphatidylglycerol, phosphatidylglycerol, one glycolipid and three unidentified lipids.
33044149	7	18	theme	Q22T	828:831	arg1	diphosphatidylglycerol					838:859	diphosphatidylglycerol	838:859	diphosphatidylglycerol	838:859	The major polar lipids of strain Q22T were diphosphatidylglycerol, phosphatidylglycerol, one glycolipid and three unidentified lipids.
33044149	2	19	theme	plant	266:270	arg1	soil					249:252	the rhizosphere soil	233:252	the rhizosphere soil of mangrove plant	233:270	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium designated Q22T was isolated from the rhizosphere soil of mangrove plant, Kandelia candel collected in Zhangzhou, Fujian province, China.
33044149	2	19	theme	plant	266:270	arg1	candel					282:287	candel	282:287	candel	282:287	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium designated Q22T was isolated from the rhizosphere soil of mangrove plant, Kandelia candel collected in Zhangzhou, Fujian province, China.
33044149	11	20	theme	1K03340T=	1325:1333	arg1	39961T					1340:1345	=MCCC 1K03340T= KCTC 39961T	1319:1345	=MCCC 1K03340T= KCTC 39961T	1319:1345	The type strain is Q22T (=MCCC 1K03340T= KCTC 39961T).
33044149	11	20	theme	1K03340T=	1325:1333	arg1	Q22T					1313:1316	Q22T	1313:1316	Q22T (=MCCC 1K03340T= KCTC 39961T)	1313:1346	The type strain is Q22T (=MCCC 1K03340T= KCTC 39961T).
33044149	4	21	theme	genomic	472:478	arg1	content					488:494	The genomic DNA G+C content	468:494	The genomic DNA G+C content	468:494	The genomic DNA G+C content was 71.9%.
33044149	4	21	theme	genomic	472:478	arg1	%					504:504	71.9%	500:504	71.9%	500:504	The genomic DNA G+C content was 71.9%.
33044149	9	22	theme	Agromyces	1222:1230	arg1	species					1201:1207	a novel species	1193:1207	a novel species	1193:1207	The phylogenetic analysis and genotypic features, along with the phenotypic and chemotaxonomic characteristics, indicate that strain Q22T represents a novel species of the genus Agromyces, for which the name Agromyces kandeliae sp.
33044149	8	23	from	glycine	1013:1019	arg1	peptidoglycans					1028:1041	the peptidoglycans	1024:1041	the peptidoglycans	1024:1041	The strain Q22T contained 2,4-diaminobutyric acid, alanine acid, glutamic acid and glycine in the peptidoglycans.
33044149	1	24	theme	Kandelia	64:71	arg1	soil					56:59	rhizosphere soil	44:59	rhizosphere soil of Kandelia	44:71	nov., isolated from rhizosphere soil of Kandelia candel in a mangrove.
33044149	5	25	dep	in	544:545	arg1	silico					547:552	silico	547:552	silico	547:552	The average nucleotide identity, and in silico DNA-DNA hybridization values between strain Q22T and the reference strains were 79.7-88.9% and 22.6-37.4%, respectively.
33044149	0	26	theme	kandeliae	10:18	arg1	sp					20:21	Agromyces kandeliae sp	0:21	Agromyces kandeliae sp.	0:22	Agromyces kandeliae sp.
33044149	2	27	theme	Gram-stain-positive	104:122	arg1	bacterium					189:197	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium	95:197	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium designated Q22T	95:213	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium designated Q22T was isolated from the rhizosphere soil of mangrove plant, Kandelia candel collected in Zhangzhou, Fujian province, China.
33044149	9	28	theme	genus	1216:1220	arg1	Agromyces					1222:1230	the genus Agromyces	1212:1230	the genus Agromyces	1212:1230	The phylogenetic analysis and genotypic features, along with the phenotypic and chemotaxonomic characteristics, indicate that strain Q22T represents a novel species of the genus Agromyces, for which the name Agromyces kandeliae sp.
33044149	3	29	theme	optimum	379:385	arg1	°C					390:391	optimum 30 °C	379:391	optimum 30 °C	379:391	Strain Q22T was able to grow at 10-40 °C (optimum 30 °C), pH 5.5-9.0 (optimum 7.0-8.0) and with 0-5.0% (w/v) NaCl (optimum 1.0 %).
33044149	3	29	theme	optimum	379:385	arg1	°C					375:376	10-40 °C	369:376	10-40 °C (optimum 30 °C)	369:392	Strain Q22T was able to grow at 10-40 °C (optimum 30 °C), pH 5.5-9.0 (optimum 7.0-8.0) and with 0-5.0% (w/v) NaCl (optimum 1.0 %).
33044149	0	30	theme	Agromyces	0:8	arg1	sp					20:21	Agromyces kandeliae sp	0:21	Agromyces kandeliae sp.	0:22	Agromyces kandeliae sp.
33044149	5	31	theme	average	511:517	arg1	identity					530:537	The average nucleotide identity	507:537	The average nucleotide identity	507:537	The average nucleotide identity, and in silico DNA-DNA hybridization values between strain Q22T and the reference strains were 79.7-88.9% and 22.6-37.4%, respectively.
33044149	7	32	theme	major	799:803	arg1	lipids					811:816	The major polar lipids	795:816	The major polar lipids of strain Q22T	795:831	The major polar lipids of strain Q22T were diphosphatidylglycerol, phosphatidylglycerol, one glycolipid and three unidentified lipids.
33044149	7	32	theme	major	799:803	arg1	diphosphatidylglycerol					838:859	diphosphatidylglycerol	838:859	diphosphatidylglycerol	838:859	The major polar lipids of strain Q22T were diphosphatidylglycerol, phosphatidylglycerol, one glycolipid and three unidentified lipids.
33044149	6	33	theme	fatty	734:738	arg1	anteiso-C15:0					751:763	anteiso-C15:0	751:763	anteiso-C15:0	751:763	The predominant isoprenoid quinone was MK-12 and the major fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0.
33044149	6	33	theme	fatty	734:738	arg1	acids					740:744	the major fatty acids	724:744	the major fatty acids	724:744	The predominant isoprenoid quinone was MK-12 and the major fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0.
33044149	9	34	theme	phenotypic	1109:1118	arg1	characteristics					1139:1153	the phenotypic and chemotaxonomic characteristics	1105:1153	the phenotypic and chemotaxonomic characteristics	1105:1153	The phylogenetic analysis and genotypic features, along with the phenotypic and chemotaxonomic characteristics, indicate that strain Q22T represents a novel species of the genus Agromyces, for which the name Agromyces kandeliae sp.
33044149	8	35	theme	alanine	981:987	arg1	acid					989:992	alanine acid	981:992	alanine acid	981:992	The strain Q22T contained 2,4-diaminobutyric acid, alanine acid, glutamic acid and glycine in the peptidoglycans.
33044149	7	36	theme	polar	805:809	arg1	lipids					811:816	The major polar lipids	795:816	The major polar lipids of strain Q22T	795:831	The major polar lipids of strain Q22T were diphosphatidylglycerol, phosphatidylglycerol, one glycolipid and three unidentified lipids.
33044149	7	36	theme	polar	805:809	arg1	diphosphatidylglycerol					838:859	diphosphatidylglycerol	838:859	diphosphatidylglycerol	838:859	The major polar lipids of strain Q22T were diphosphatidylglycerol, phosphatidylglycerol, one glycolipid and three unidentified lipids.
33044149	3	37	theme	optimum	452:458	arg1	NaCl					446:449	0-5.0% (w/v) NaCl	433:449	0-5.0% (w/v) NaCl (optimum 1.0 %)	433:465	Strain Q22T was able to grow at 10-40 °C (optimum 30 °C), pH 5.5-9.0 (optimum 7.0-8.0) and with 0-5.0% (w/v) NaCl (optimum 1.0 %).
33044149	3	37	theme	optimum	452:458	arg1	%					464:464	optimum 1.0 %	452:464	optimum 1.0 %	452:464	Strain Q22T was able to grow at 10-40 °C (optimum 30 °C), pH 5.5-9.0 (optimum 7.0-8.0) and with 0-5.0% (w/v) NaCl (optimum 1.0 %).
33044149	9	38	theme	genotypic	1074:1082	arg1	features					1084:1091	genotypic features	1074:1091	genotypic features	1074:1091	The phylogenetic analysis and genotypic features, along with the phenotypic and chemotaxonomic characteristics, indicate that strain Q22T represents a novel species of the genus Agromyces, for which the name Agromyces kandeliae sp.
33044149	3	39	theme	w/v	441:443	arg1	NaCl					446:449	0-5.0% (w/v) NaCl	433:449	0-5.0% (w/v) NaCl (optimum 1.0 %)	433:465	Strain Q22T was able to grow at 10-40 °C (optimum 30 °C), pH 5.5-9.0 (optimum 7.0-8.0) and with 0-5.0% (w/v) NaCl (optimum 1.0 %).
33044149	3	39	theme	w/v	441:443	arg1	%					464:464	optimum 1.0 %	452:464	optimum 1.0 %	452:464	Strain Q22T was able to grow at 10-40 °C (optimum 30 °C), pH 5.5-9.0 (optimum 7.0-8.0) and with 0-5.0% (w/v) NaCl (optimum 1.0 %).
33044149	9	40	theme	chemotaxonomic	1124:1137	arg1	characteristics					1139:1153	the phenotypic and chemotaxonomic characteristics	1105:1153	the phenotypic and chemotaxonomic characteristics	1105:1153	The phylogenetic analysis and genotypic features, along with the phenotypic and chemotaxonomic characteristics, indicate that strain Q22T represents a novel species of the genus Agromyces, for which the name Agromyces kandeliae sp.
33044149	3	41	theme	1.0 	460:463	arg1	NaCl					446:449	0-5.0% (w/v) NaCl	433:449	0-5.0% (w/v) NaCl (optimum 1.0 %)	433:465	Strain Q22T was able to grow at 10-40 °C (optimum 30 °C), pH 5.5-9.0 (optimum 7.0-8.0) and with 0-5.0% (w/v) NaCl (optimum 1.0 %).
33044149	3	41	theme	1.0 	460:463	arg1	%					464:464	optimum 1.0 %	452:464	optimum 1.0 %	452:464	Strain Q22T was able to grow at 10-40 °C (optimum 30 °C), pH 5.5-9.0 (optimum 7.0-8.0) and with 0-5.0% (w/v) NaCl (optimum 1.0 %).
33044149	11	42	theme	KCTC	1335:1338	arg1	39961T					1340:1345	=MCCC 1K03340T= KCTC 39961T	1319:1345	=MCCC 1K03340T= KCTC 39961T	1319:1345	The type strain is Q22T (=MCCC 1K03340T= KCTC 39961T).
33044149	11	42	theme	KCTC	1335:1338	arg1	Q22T					1313:1316	Q22T	1313:1316	Q22T (=MCCC 1K03340T= KCTC 39961T)	1313:1346	The type strain is Q22T (=MCCC 1K03340T= KCTC 39961T).
33044149	9	43	theme	name	1247:1250	arg1	sp					1272:1273	the name Agromyces kandeliae sp	1243:1273	the name Agromyces kandeliae sp	1243:1273	The phylogenetic analysis and genotypic features, along with the phenotypic and chemotaxonomic characteristics, indicate that strain Q22T represents a novel species of the genus Agromyces, for which the name Agromyces kandeliae sp.
33044149	5	44	theme	strain	591:596	arg1	Q22T					598:601	strain Q22T	591:601	strain Q22T	591:601	The average nucleotide identity, and in silico DNA-DNA hybridization values between strain Q22T and the reference strains were 79.7-88.9% and 22.6-37.4%, respectively.
33044149	2	45	theme	non-motile	153:162	arg1	bacterium					189:197	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium	95:197	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium designated Q22T	95:213	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium designated Q22T was isolated from the rhizosphere soil of mangrove plant, Kandelia candel collected in Zhangzhou, Fujian province, China.
33044149	8	46	contain	contained	946:954	arg2	acid					975:978	2,4-diaminobutyric acid	956:978	2,4-diaminobutyric acid	956:978	The strain Q22T contained 2,4-diaminobutyric acid, alanine acid, glutamic acid and glycine in the peptidoglycans.
33044149	8	46	contain	contained	946:954	arg2	glycine					1013:1019	glycine	1013:1019	glycine	1013:1019	The strain Q22T contained 2,4-diaminobutyric acid, alanine acid, glutamic acid and glycine in the peptidoglycans.
33044149	8	46	contain	contained	946:954	arg2	acid					1004:1007	glutamic acid	995:1007	glutamic acid	995:1007	The strain Q22T contained 2,4-diaminobutyric acid, alanine acid, glutamic acid and glycine in the peptidoglycans.
33044149	8	46	contain	contained	946:954	arg1	Q22T					941:944	The strain Q22T	930:944	The strain Q22T	930:944	The strain Q22T contained 2,4-diaminobutyric acid, alanine acid, glutamic acid and glycine in the peptidoglycans.
33044149	8	46	contain	contained	946:954	arg2	acid					989:992	alanine acid	981:992	alanine acid	981:992	The strain Q22T contained 2,4-diaminobutyric acid, alanine acid, glutamic acid and glycine in the peptidoglycans.
33044149	8	47	from	acid	975:978	arg1	peptidoglycans					1028:1041	the peptidoglycans	1024:1041	the peptidoglycans	1024:1041	The strain Q22T contained 2,4-diaminobutyric acid, alanine acid, glutamic acid and glycine in the peptidoglycans.
33044149	6	48	theme	major	728:732	arg1	anteiso-C15:0					751:763	anteiso-C15:0	751:763	anteiso-C15:0	751:763	The predominant isoprenoid quinone was MK-12 and the major fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0.
33044149	6	48	theme	major	728:732	arg1	acids					740:744	the major fatty acids	724:744	the major fatty acids	724:744	The predominant isoprenoid quinone was MK-12 and the major fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0.
33044149	11	49	theme	type	1298:1301	arg1	Q22T					1313:1316	Q22T	1313:1316	Q22T (=MCCC 1K03340T= KCTC 39961T)	1313:1346	The type strain is Q22T (=MCCC 1K03340T= KCTC 39961T).
33044149	11	49	theme	type	1298:1301	arg1	strain					1303:1308	The type strain	1294:1308	The type strain	1294:1308	The type strain is Q22T (=MCCC 1K03340T= KCTC 39961T).
33044149	9	50	theme	Agromyces	1252:1260	arg1	sp					1272:1273	the name Agromyces kandeliae sp	1243:1273	the name Agromyces kandeliae sp	1243:1273	The phylogenetic analysis and genotypic features, along with the phenotypic and chemotaxonomic characteristics, indicate that strain Q22T represents a novel species of the genus Agromyces, for which the name Agromyces kandeliae sp.
33044149	5	51	theme	in	544:545	arg1	hybridization					562:574	in silico DNA-DNA hybridization	544:574	in silico DNA-DNA hybridization	544:574	The average nucleotide identity, and in silico DNA-DNA hybridization values between strain Q22T and the reference strains were 79.7-88.9% and 22.6-37.4%, respectively.
33044149	2	52	theme	non-spore-forming	134:150	arg1	bacterium					189:197	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium	95:197	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium designated Q22T	95:213	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium designated Q22T was isolated from the rhizosphere soil of mangrove plant, Kandelia candel collected in Zhangzhou, Fujian province, China.
33044149	7	53	theme	unidentified	909:920	arg1	lipids					922:927	three unidentified lipids	903:927	three unidentified lipids	903:927	The major polar lipids of strain Q22T were diphosphatidylglycerol, phosphatidylglycerol, one glycolipid and three unidentified lipids.
33044149	8	54	theme	glutamic	995:1002	arg1	acid					1004:1007	glutamic acid	995:1007	glutamic acid	995:1007	The strain Q22T contained 2,4-diaminobutyric acid, alanine acid, glutamic acid and glycine in the peptidoglycans.
33044149	5	55	theme	DNA-DNA	554:560	arg1	hybridization					562:574	in silico DNA-DNA hybridization	544:574	in silico DNA-DNA hybridization	544:574	The average nucleotide identity, and in silico DNA-DNA hybridization values between strain Q22T and the reference strains were 79.7-88.9% and 22.6-37.4%, respectively.
33044149	9	56	theme	strain	1170:1175	arg1	Q22T					1177:1180	strain Q22T	1170:1180	strain Q22T	1170:1180	The phylogenetic analysis and genotypic features, along with the phenotypic and chemotaxonomic characteristics, indicate that strain Q22T represents a novel species of the genus Agromyces, for which the name Agromyces kandeliae sp.
33044149	8	57	from	acid	989:992	arg1	peptidoglycans					1028:1041	the peptidoglycans	1024:1041	the peptidoglycans	1024:1041	The strain Q22T contained 2,4-diaminobutyric acid, alanine acid, glutamic acid and glycine in the peptidoglycans.
33044149	3	58	theme	optimum	407:413	arg1	pH					395:396	pH 5.5-9.0	395:404	pH 5.5-9.0 (optimum 7.0-8.0)	395:422	Strain Q22T was able to grow at 10-40 °C (optimum 30 °C), pH 5.5-9.0 (optimum 7.0-8.0) and with 0-5.0% (w/v) NaCl (optimum 1.0 %).
33044149	3	58	theme	optimum	407:413	arg1	7.0-8.0					415:421	optimum 7.0-8.0	407:421	optimum 7.0-8.0	407:421	Strain Q22T was able to grow at 10-40 °C (optimum 30 °C), pH 5.5-9.0 (optimum 7.0-8.0) and with 0-5.0% (w/v) NaCl (optimum 1.0 %).
33044149	9	59	dep	along	1094:1098	arg1	with					1100:1103	with	1100:1103	with	1100:1103	The phylogenetic analysis and genotypic features, along with the phenotypic and chemotaxonomic characteristics, indicate that strain Q22T represents a novel species of the genus Agromyces, for which the name Agromyces kandeliae sp.
33044149	2	60	theme	mangrove	257:264	arg1	plant					266:270	mangrove plant	257:270	mangrove plant	257:270	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium designated Q22T was isolated from the rhizosphere soil of mangrove plant, Kandelia candel collected in Zhangzhou, Fujian province, China.
33044149	2	61	theme	rod-shaped	178:187	arg1	bacterium					189:197	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium	95:197	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium designated Q22T	95:213	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium designated Q22T was isolated from the rhizosphere soil of mangrove plant, Kandelia candel collected in Zhangzhou, Fujian province, China.
33044149	4	62	theme	G+C	484:486	arg1	content					488:494	The genomic DNA G+C content	468:494	The genomic DNA G+C content	468:494	The genomic DNA G+C content was 71.9%.
33044149	4	62	theme	G+C	484:486	arg1	%					504:504	71.9%	500:504	71.9%	500:504	The genomic DNA G+C content was 71.9%.
33044149	2	63	theme	irregular	168:176	arg1	bacterium					189:197	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium	95:197	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium designated Q22T	95:213	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium designated Q22T was isolated from the rhizosphere soil of mangrove plant, Kandelia candel collected in Zhangzhou, Fujian province, China.
33044149	2	64	theme	rhizosphere	237:247	arg1	soil					249:252	the rhizosphere soil	233:252	the rhizosphere soil of mangrove plant	233:270	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium designated Q22T was isolated from the rhizosphere soil of mangrove plant, Kandelia candel collected in Zhangzhou, Fujian province, China.
33044149	2	64	theme	rhizosphere	237:247	arg1	candel					282:287	candel	282:287	candel	282:287	A novel, Gram-stain-positive, aerobic, non-spore-forming, non-motile and irregular rod-shaped bacterium designated Q22T was isolated from the rhizosphere soil of mangrove plant, Kandelia candel collected in Zhangzhou, Fujian province, China.
33044149	9	65	theme	phylogenetic	1048:1059	arg1	analysis					1061:1068	The phylogenetic analysis	1044:1068	The phylogenetic analysis	1044:1068	The phylogenetic analysis and genotypic features, along with the phenotypic and chemotaxonomic characteristics, indicate that strain Q22T represents a novel species of the genus Agromyces, for which the name Agromyces kandeliae sp.
33044149	3	66	theme	%	438:438	arg1	NaCl					446:449	0-5.0% (w/v) NaCl	433:449	0-5.0% (w/v) NaCl (optimum 1.0 %)	433:465	Strain Q22T was able to grow at 10-40 °C (optimum 30 °C), pH 5.5-9.0 (optimum 7.0-8.0) and with 0-5.0% (w/v) NaCl (optimum 1.0 %).
33044149	3	66	theme	%	438:438	arg1	%					464:464	optimum 1.0 %	452:464	optimum 1.0 %	452:464	Strain Q22T was able to grow at 10-40 °C (optimum 30 °C), pH 5.5-9.0 (optimum 7.0-8.0) and with 0-5.0% (w/v) NaCl (optimum 1.0 %).
33044149	8	67	from	acid	1004:1007	arg1	peptidoglycans					1028:1041	the peptidoglycans	1024:1041	the peptidoglycans	1024:1041	The strain Q22T contained 2,4-diaminobutyric acid, alanine acid, glutamic acid and glycine in the peptidoglycans.
33044149	9	68	dep	Agromyces	1252:1260	arg1	kandeliae					1262:1270	kandeliae	1262:1270	kandeliae	1262:1270	The phylogenetic analysis and genotypic features, along with the phenotypic and chemotaxonomic characteristics, indicate that strain Q22T represents a novel species of the genus Agromyces, for which the name Agromyces kandeliae sp.
31976851	14	0	from	2JSPR-7T	1752:1759	arg1	genus					1768:1772	the genus	1764:1772	the genus	1764:1772	Therefore, it is proposed to combine the genera Xylanimicrobium and Xylanibacterium with the genus Xylanimonas considering the priority of publication and to classify strain 2JSPR-7T in the genus as Xylanimonas allomyrinae sp.
31976851	14	1	theme	genera	1619:1624	arg1	Xylanimicrobium					1626:1640	the genera Xylanimicrobium	1615:1640	the genera Xylanimicrobium	1615:1640	Therefore, it is proposed to combine the genera Xylanimicrobium and Xylanibacterium with the genus Xylanimonas considering the priority of publication and to classify strain 2JSPR-7T in the genus as Xylanimonas allomyrinae sp.
31976851	12	2	theme	72.7 mol	1319:1326	arg1	content					1307:1313	The DNA G+C content	1295:1313	The DNA G+C content	1295:1313	The DNA G+C content was 72.7 mol%.
31976851	12	2	theme	72.7 mol	1319:1326	arg1	%					1327:1327	72.7 mol%	1319:1327	72.7 mol%	1319:1327	The DNA G+C content was 72.7 mol%.
31976851	6	3	theme	Optimum	769:775	arg1	conditions					784:793	Optimum growth conditions	769:793	Optimum growth conditions	769:793	Optimum growth conditions were at 28-30 °C, pH 7-8 and 0 % salt concentration.
31976851	10	4	theme	peptidoglycan	1105:1117	arg1	structure					1119:1127	The peptidoglycan structure	1101:1127	The peptidoglycan structure	1101:1127	The peptidoglycan structure was suggested to be the type A3α (A11.14) l-Lys-l-Ser with the presence of d-Ala, l-Ala, d-Glu, l-Ser and l-Lys.
31976851	10	4	theme	peptidoglycan	1105:1117	arg1	l-Lys-l-Ser					1171:1181	the type A3α (A11.14) l-Lys-l-Ser	1149:1181	the type A3α (A11.14) l-Lys-l-Ser with the presence of d-Ala, l-Ala, d-Glu, l-Ser and l-Lys	1149:1239	The peptidoglycan structure was suggested to be the type A3α (A11.14) l-Lys-l-Ser with the presence of d-Ala, l-Ala, d-Glu, l-Ser and l-Lys.
31976851	9	5	theme	major	1054:1058	arg1	MK-8					1078:1081	MK-8	1078:1081	MK-8	1078:1081	The major menaquinones were MK-8(H4) and MK-9(H4).
31976851	9	5	theme	major	1054:1058	arg1	menaquinones					1060:1071	The major menaquinones	1050:1071	The major menaquinones	1050:1071	The major menaquinones were MK-8(H4) and MK-9(H4).
31976851	12	6	theme	DNA	1299:1301	arg1	content					1307:1313	The DNA G+C content	1295:1313	The DNA G+C content	1295:1313	The DNA G+C content was 72.7 mol%.
31976851	12	6	theme	DNA	1299:1301	arg1	%					1327:1327	72.7 mol%	1319:1327	72.7 mol%	1319:1327	The DNA G+C content was 72.7 mol%.
31976851	5	7	theme	Xylanibacterium	630:644	arg1	XIL08T					651:656	Xylanibacterium ulmi XIL08T	630:656	Xylanibacterium ulmi XIL08T (98.1 %)	630:665	2JSPR-7T had the highest 16S rRNA gene sequence similarity to Xylanibacterium ulmi XIL08T (98.1 %), Xylanimicrobium pachnodae NBRC 107786T (97.8 %) and Xylanimonas cellulosilytica DSM 15894T (97.5 %).
31976851	5	7	theme	Xylanibacterium	630:644	arg1	%					664:664	98.1 %	659:664	98.1 %	659:664	2JSPR-7T had the highest 16S rRNA gene sequence similarity to Xylanibacterium ulmi XIL08T (98.1 %), Xylanimicrobium pachnodae NBRC 107786T (97.8 %) and Xylanimonas cellulosilytica DSM 15894T (97.5 %).
31976851	5	8	theme	NBRC	694:697	arg1	107786T					699:705	Xylanimicrobium pachnodae NBRC 107786T	668:705	Xylanimicrobium pachnodae NBRC 107786T (97.8 %)	668:714	2JSPR-7T had the highest 16S rRNA gene sequence similarity to Xylanibacterium ulmi XIL08T (98.1 %), Xylanimicrobium pachnodae NBRC 107786T (97.8 %) and Xylanimonas cellulosilytica DSM 15894T (97.5 %).
31976851	5	8	theme	NBRC	694:697	arg1	%					713:713	97.8 %	708:713	97.8 %	708:713	2JSPR-7T had the highest 16S rRNA gene sequence similarity to Xylanibacterium ulmi XIL08T (98.1 %), Xylanimicrobium pachnodae NBRC 107786T (97.8 %) and Xylanimonas cellulosilytica DSM 15894T (97.5 %).
31976851	3	9	theme	Xylanimonas	256:266	arg1	nov.					222:225	nov.	222:225	nov.	222:225	nov., and emendation of the genus Xylanimonas.
31976851	3	9	theme	Xylanimonas	256:266	arg1	emendation					232:241	emendation	232:241	emendation	232:241	nov., and emendation of the genus Xylanimonas.
31976851	4	10	theme	larvae	437:442	arg1	gut					430:432	a gut	428:432	a gut of larvae of Allomyrina dichotoma which were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea	428:565	A bacterium that was Gram-staining-positive, facultatively anaerobic, non-motile, rod- or filamentous-shaped, designated as strain 2JSPR-7T, was isolated from a gut of larvae of Allomyrina dichotoma which were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
31976851	11	11	theme	Whole	1242:1246	arg1	sugars					1253:1258	Whole cell sugars	1242:1258	Whole cell sugars	1242:1258	Whole cell sugars were rhamnose, ribose and glucose.
31976851	14	12	theme	Xylanimonas	1777:1787	arg1	sp					1801:1802	Xylanimonas allomyrinae sp	1777:1802	Xylanimonas allomyrinae sp	1777:1802	Therefore, it is proposed to combine the genera Xylanimicrobium and Xylanibacterium with the genus Xylanimonas considering the priority of publication and to classify strain 2JSPR-7T in the genus as Xylanimonas allomyrinae sp.
31976851	4	13	theme	Allomyrina	447:456	arg1	dichotoma					458:466	Allomyrina dichotoma	447:466	Allomyrina dichotoma	447:466	A bacterium that was Gram-staining-positive, facultatively anaerobic, non-motile, rod- or filamentous-shaped, designated as strain 2JSPR-7T, was isolated from a gut of larvae of Allomyrina dichotoma which were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
31976851	1	14	theme	Xylanibacterium	109:123	arg1	ulmi					125:128	Xylanibacterium ulmi	109:128	Xylanibacterium ulmi as Xylanimonas ulmi comb	109:153	nov. isolated from the gut of larva of Allomyrina dichotoma, reclassification of Xylanibacterium ulmi as Xylanimonas ulmi comb.
31976851	5	15	theme	Xylanimicrobium	668:682	arg1	107786T					699:705	Xylanimicrobium pachnodae NBRC 107786T	668:705	Xylanimicrobium pachnodae NBRC 107786T (97.8 %)	668:714	2JSPR-7T had the highest 16S rRNA gene sequence similarity to Xylanibacterium ulmi XIL08T (98.1 %), Xylanimicrobium pachnodae NBRC 107786T (97.8 %) and Xylanimonas cellulosilytica DSM 15894T (97.5 %).
31976851	5	15	theme	Xylanimicrobium	668:682	arg1	%					713:713	97.8 %	708:713	97.8 %	708:713	2JSPR-7T had the highest 16S rRNA gene sequence similarity to Xylanibacterium ulmi XIL08T (98.1 %), Xylanimicrobium pachnodae NBRC 107786T (97.8 %) and Xylanimonas cellulosilytica DSM 15894T (97.5 %).
31976851	17	16	theme	genus	1924:1928	arg1	Xylanimonas					1930:1940	the genus Xylanimonas	1920:1940	the genus Xylanimonas	1920:1940	In addition, the description of the genus Xylanimonas is emended, and Xylanibacterium ulmi and Xylanimicrobium pachnodae are reclassified as Xylanimonas ulmi comb.
31976851	13	17	theme	strain	1401:1406	arg1	2JSPR-7T					1408:1415	strain 2JSPR-7T	1401:1415	strain 2JSPR-7T from any of the genera Xylanimonas, Xylanimicrobium and Xylanibacterium based on the polyphasic approach including phylogenetic and phenotypic characterization	1401:1575	We encountered difficulty in selecting a suitable genus to accommodate strain 2JSPR-7T from any of the genera Xylanimonas, Xylanimicrobium and Xylanibacterium based on the polyphasic approach including phylogenetic and phenotypic characterization.
31976851	14	18	theme	strain	1745:1750	arg1	2JSPR-7T					1752:1759	strain 2JSPR-7T	1745:1759	strain 2JSPR-7T in the genus	1745:1772	Therefore, it is proposed to combine the genera Xylanimicrobium and Xylanibacterium with the genus Xylanimonas considering the priority of publication and to classify strain 2JSPR-7T in the genus as Xylanimonas allomyrinae sp.
31976851	10	19	theme	A11.14	1163:1168	arg1	structure					1119:1127	The peptidoglycan structure	1101:1127	The peptidoglycan structure	1101:1127	The peptidoglycan structure was suggested to be the type A3α (A11.14) l-Lys-l-Ser with the presence of d-Ala, l-Ala, d-Glu, l-Ser and l-Lys.
31976851	10	19	theme	A11.14	1163:1168	arg1	l-Lys-l-Ser					1171:1181	the type A3α (A11.14) l-Lys-l-Ser	1149:1181	the type A3α (A11.14) l-Lys-l-Ser with the presence of d-Ala, l-Ala, d-Glu, l-Ser and l-Lys	1149:1239	The peptidoglycan structure was suggested to be the type A3α (A11.14) l-Lys-l-Ser with the presence of d-Ala, l-Ala, d-Glu, l-Ser and l-Lys.
31976851	8	20	theme	polar	935:939	arg1	diphosphatidylglycerol					953:974	diphosphatidylglycerol	953:974	diphosphatidylglycerol	953:974	The polar lipids were diphosphatidylglycerol, four unidentified phospholipids and two unidentified glycophospholipids.
31976851	8	20	theme	polar	935:939	arg1	lipids					941:946	The polar lipids	931:946	The polar lipids	931:946	The polar lipids were diphosphatidylglycerol, four unidentified phospholipids and two unidentified glycophospholipids.
31976851	5	21	theme	highest	585:591	arg1	similarity					616:625	the highest 16S rRNA gene sequence similarity	581:625	the highest 16S rRNA gene sequence similarity to Xylanibacterium ulmi XIL08T (98.1 %), Xylanimicrobium pachnodae NBRC 107786T (97.8 %) and Xylanimonas cellulosilytica DSM 15894T (97.5 %)	581:766	2JSPR-7T had the highest 16S rRNA gene sequence similarity to Xylanibacterium ulmi XIL08T (98.1 %), Xylanimicrobium pachnodae NBRC 107786T (97.8 %) and Xylanimonas cellulosilytica DSM 15894T (97.5 %).
31976851	10	22	theme	A3α	1158:1160	arg1	structure					1119:1127	The peptidoglycan structure	1101:1127	The peptidoglycan structure	1101:1127	The peptidoglycan structure was suggested to be the type A3α (A11.14) l-Lys-l-Ser with the presence of d-Ala, l-Ala, d-Glu, l-Ser and l-Lys.
31976851	10	22	theme	A3α	1158:1160	arg1	l-Lys-l-Ser					1171:1181	the type A3α (A11.14) l-Lys-l-Ser	1149:1181	the type A3α (A11.14) l-Lys-l-Ser with the presence of d-Ala, l-Ala, d-Glu, l-Ser and l-Lys	1149:1239	The peptidoglycan structure was suggested to be the type A3α (A11.14) l-Lys-l-Ser with the presence of d-Ala, l-Ala, d-Glu, l-Ser and l-Lys.
31976851	5	23	theme	rRNA	597:600	arg1	similarity					616:625	the highest 16S rRNA gene sequence similarity	581:625	the highest 16S rRNA gene sequence similarity to Xylanibacterium ulmi XIL08T (98.1 %), Xylanimicrobium pachnodae NBRC 107786T (97.8 %) and Xylanimonas cellulosilytica DSM 15894T (97.5 %)	581:766	2JSPR-7T had the highest 16S rRNA gene sequence similarity to Xylanibacterium ulmi XIL08T (98.1 %), Xylanimicrobium pachnodae NBRC 107786T (97.8 %) and Xylanimonas cellulosilytica DSM 15894T (97.5 %).
31976851	16	24	theme	=KACC	1860:1864	arg1	113052T					1878:1884	=KACC 19330T=NBRC 113052T	1860:1884	=KACC 19330T=NBRC 113052T	1860:1884	The type strain of the novel species is 2JSPR-7T (=KACC 19330T=NBRC 113052T).
31976851	16	24	theme	=KACC	1860:1864	arg1	2JSPR-7T					1850:1857	2JSPR-7T	1850:1857	2JSPR-7T (=KACC 19330T=NBRC 113052T)	1850:1885	The type strain of the novel species is 2JSPR-7T (=KACC 19330T=NBRC 113052T).
31976851	7	25	dep	 0	927:928	arg1	C16 					922:925	C16 	922:925	C16 	922:925	The cellular fatty acids mainly consisted of anteiso-C15 : 0, C14 : 0 and C16 : 0.
31976851	7	25	dep	 0	927:928	arg1	 0					915:916	 0	915:916	 0	915:916	The cellular fatty acids mainly consisted of anteiso-C15 : 0, C14 : 0 and C16 : 0.
31976851	7	25	dep	 0	927:928	arg1	C14 					910:913	C14 	910:913	C14 	910:913	The cellular fatty acids mainly consisted of anteiso-C15 : 0, C14 : 0 and C16 : 0.
31976851	0	26	theme	Xylanimonas	0:10	arg1	sp					24:25	Xylanimonas allomyrinae sp	0:25	Xylanimonas allomyrinae sp.	0:26	Xylanimonas allomyrinae sp.
31976851	8	27	theme	unidentified	982:993	arg1	diphosphatidylglycerol					953:974	diphosphatidylglycerol	953:974	diphosphatidylglycerol	953:974	The polar lipids were diphosphatidylglycerol, four unidentified phospholipids and two unidentified glycophospholipids.
31976851	8	27	theme	unidentified	982:993	arg1	phospholipids					995:1007	four unidentified phospholipids	977:1007	four unidentified phospholipids	977:1007	The polar lipids were diphosphatidylglycerol, four unidentified phospholipids and two unidentified glycophospholipids.
31976851	1	28	theme	dichotoma	78:86	arg1	larva					58:62	larva	58:62	larva of Allomyrina dichotoma	58:86	nov. isolated from the gut of larva of Allomyrina dichotoma, reclassification of Xylanibacterium ulmi as Xylanimonas ulmi comb.
31976851	1	28	theme	dichotoma	78:86	arg1	reclassification					89:104	reclassification	89:104	reclassification of Xylanibacterium ulmi as Xylanimonas ulmi comb	89:153	nov. isolated from the gut of larva of Allomyrina dichotoma, reclassification of Xylanibacterium ulmi as Xylanimonas ulmi comb.
31976851	6	29	theme	0	824:824	arg1	%					826:826	%	826:826	%	826:826	Optimum growth conditions were at 28-30 °C, pH 7-8 and 0 % salt concentration.
31976851	2	30	theme	Xylanimicrobium	165:179	arg1	pachnodae					181:189	Xylanimicrobium pachnodae	165:189	Xylanimicrobium pachnodae	165:189	nov. and Xylanimicrobium pachnodae as Xylanimonas pachnodae comb.
31976851	8	31	theme	unidentified	1017:1028	arg1	glycophospholipids					1030:1047	two unidentified glycophospholipids	1013:1047	two unidentified glycophospholipids	1013:1047	The polar lipids were diphosphatidylglycerol, four unidentified phospholipids and two unidentified glycophospholipids.
31976851	8	31	theme	unidentified	1017:1028	arg1	diphosphatidylglycerol					953:974	diphosphatidylglycerol	953:974	diphosphatidylglycerol	953:974	The polar lipids were diphosphatidylglycerol, four unidentified phospholipids and two unidentified glycophospholipids.
31976851	17	32	theme	Xylanimonas	2029:2039	arg1	ulmi					1974:1977	ulmi	1974:1977	ulmi	1974:1977	In addition, the description of the genus Xylanimonas is emended, and Xylanibacterium ulmi and Xylanimicrobium pachnodae are reclassified as Xylanimonas ulmi comb.
31976851	17	32	theme	Xylanimonas	2029:2039	arg1	pachnodae					1999:2007	pachnodae	1999:2007	pachnodae	1999:2007	In addition, the description of the genus Xylanimonas is emended, and Xylanibacterium ulmi and Xylanimicrobium pachnodae are reclassified as Xylanimonas ulmi comb.
31976851	17	32	theme	Xylanimonas	2029:2039	arg1	comb					2046:2049	Xylanimonas ulmi comb	2029:2049	Xylanimonas ulmi comb	2029:2049	In addition, the description of the genus Xylanimonas is emended, and Xylanibacterium ulmi and Xylanimicrobium pachnodae are reclassified as Xylanimonas ulmi comb.
31976851	12	33	theme	G+C	1303:1305	arg1	content					1307:1313	The DNA G+C content	1295:1313	The DNA G+C content	1295:1313	The DNA G+C content was 72.7 mol%.
31976851	12	33	theme	G+C	1303:1305	arg1	%					1327:1327	72.7 mol%	1319:1327	72.7 mol%	1319:1327	The DNA G+C content was 72.7 mol%.
31976851	13	34	theme	polyphasic	1502:1511	arg1	approach					1513:1520	the polyphasic approach	1498:1520	the polyphasic approach including phylogenetic and phenotypic characterization	1498:1575	We encountered difficulty in selecting a suitable genus to accommodate strain 2JSPR-7T from any of the genera Xylanimonas, Xylanimicrobium and Xylanibacterium based on the polyphasic approach including phylogenetic and phenotypic characterization.
31976851	2	35	theme	pachnodae	206:214	arg1	comb					216:219	Xylanimonas pachnodae comb	194:219	Xylanimonas pachnodae comb	194:219	nov. and Xylanimicrobium pachnodae as Xylanimonas pachnodae comb.
31976851	16	36	theme	species	1839:1845	arg1	strain					1819:1824	The type strain	1810:1824	The type strain of the novel species	1810:1845	The type strain of the novel species is 2JSPR-7T (=KACC 19330T=NBRC 113052T).
31976851	16	36	theme	species	1839:1845	arg1	2JSPR-7T					1850:1857	2JSPR-7T	1850:1857	2JSPR-7T (=KACC 19330T=NBRC 113052T)	1850:1885	The type strain of the novel species is 2JSPR-7T (=KACC 19330T=NBRC 113052T).
31976851	1	37	theme	ulmi	145:148	arg1	comb					150:153	Xylanimonas ulmi comb	133:153	Xylanimonas ulmi comb	133:153	nov. isolated from the gut of larva of Allomyrina dichotoma, reclassification of Xylanibacterium ulmi as Xylanimonas ulmi comb.
31976851	7	38	theme	cellular	852:859	arg1	acids					867:871	The cellular fatty acids	848:871	The cellular fatty acids	848:871	The cellular fatty acids mainly consisted of anteiso-C15 : 0, C14 : 0 and C16 : 0.
31976851	11	39	theme	cell	1248:1251	arg1	sugars					1253:1258	Whole cell sugars	1242:1258	Whole cell sugars	1242:1258	Whole cell sugars were rhamnose, ribose and glucose.
31976851	5	40	theme	ulmi	646:649	arg1	XIL08T					651:656	Xylanibacterium ulmi XIL08T	630:656	Xylanibacterium ulmi XIL08T (98.1 %)	630:665	2JSPR-7T had the highest 16S rRNA gene sequence similarity to Xylanibacterium ulmi XIL08T (98.1 %), Xylanimicrobium pachnodae NBRC 107786T (97.8 %) and Xylanimonas cellulosilytica DSM 15894T (97.5 %).
31976851	5	40	theme	ulmi	646:649	arg1	%					664:664	98.1 %	659:664	98.1 %	659:664	2JSPR-7T had the highest 16S rRNA gene sequence similarity to Xylanibacterium ulmi XIL08T (98.1 %), Xylanimicrobium pachnodae NBRC 107786T (97.8 %) and Xylanimonas cellulosilytica DSM 15894T (97.5 %).
31976851	6	41	theme	%	826:826	arg1	concentration					833:845	0 % salt concentration	824:845	0 % salt concentration	824:845	Optimum growth conditions were at 28-30 °C, pH 7-8 and 0 % salt concentration.
31976851	13	42	dep	genera	1433:1438	arg1	Xylanibacterium					1473:1487	Xylanibacterium	1473:1487	Xylanibacterium	1473:1487	We encountered difficulty in selecting a suitable genus to accommodate strain 2JSPR-7T from any of the genera Xylanimonas, Xylanimicrobium and Xylanibacterium based on the polyphasic approach including phylogenetic and phenotypic characterization.
31976851	13	42	dep	genera	1433:1438	arg1	Xylanimicrobium					1453:1467	Xylanimicrobium	1453:1467	Xylanimicrobium	1453:1467	We encountered difficulty in selecting a suitable genus to accommodate strain 2JSPR-7T from any of the genera Xylanimonas, Xylanimicrobium and Xylanibacterium based on the polyphasic approach including phylogenetic and phenotypic characterization.
31976851	6	43	theme	growth	777:782	arg1	conditions					784:793	Optimum growth conditions	769:793	Optimum growth conditions	769:793	Optimum growth conditions were at 28-30 °C, pH 7-8 and 0 % salt concentration.
31976851	5	44	theme	sequence	607:614	arg1	similarity					616:625	the highest 16S rRNA gene sequence similarity	581:625	the highest 16S rRNA gene sequence similarity to Xylanibacterium ulmi XIL08T (98.1 %), Xylanimicrobium pachnodae NBRC 107786T (97.8 %) and Xylanimonas cellulosilytica DSM 15894T (97.5 %)	581:766	2JSPR-7T had the highest 16S rRNA gene sequence similarity to Xylanibacterium ulmi XIL08T (98.1 %), Xylanimicrobium pachnodae NBRC 107786T (97.8 %) and Xylanimonas cellulosilytica DSM 15894T (97.5 %).
31976851	6	45	theme	salt	828:831	arg1	concentration					833:845	0 % salt concentration	824:845	0 % salt concentration	824:845	Optimum growth conditions were at 28-30 °C, pH 7-8 and 0 % salt concentration.
31976851	3	46	theme	genus	250:254	arg1	Xylanimonas					256:266	the genus Xylanimonas	246:266	the genus Xylanimonas	246:266	nov., and emendation of the genus Xylanimonas.
31976851	18	47	dep	comb	2083:2086	arg1	Xylanimonas					2061:2071	Xylanimonas	2061:2071	Xylanimonas	2061:2071	nov. and Xylanimonas pachnodae comb.
31976851	18	47	dep	comb	2083:2086	arg1	nov.					2052:2055	nov.	2052:2055	nov.	2052:2055	nov. and Xylanimonas pachnodae comb.
31976851	4	48	theme	strain	393:398	arg1	2JSPR-7T					400:407	strain 2JSPR-7T	393:407	strain 2JSPR-7T	393:407	A bacterium that was Gram-staining-positive, facultatively anaerobic, non-motile, rod- or filamentous-shaped, designated as strain 2JSPR-7T, was isolated from a gut of larvae of Allomyrina dichotoma which were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
31976851	13	49	from	any	1422:1424	arg1	2JSPR-7T					1408:1415	strain 2JSPR-7T	1401:1415	strain 2JSPR-7T from any of the genera Xylanimonas, Xylanimicrobium and Xylanibacterium based on the polyphasic approach including phylogenetic and phenotypic characterization	1401:1575	We encountered difficulty in selecting a suitable genus to accommodate strain 2JSPR-7T from any of the genera Xylanimonas, Xylanimicrobium and Xylanibacterium based on the polyphasic approach including phylogenetic and phenotypic characterization.
31976851	10	50	with	l-Lys-l-Ser	1171:1181	arg1	presence					1192:1199	the presence	1188:1199	the presence of d-Ala, l-Ala, d-Glu, l-Ser and l-Lys	1188:1239	The peptidoglycan structure was suggested to be the type A3α (A11.14) l-Lys-l-Ser with the presence of d-Ala, l-Ala, d-Glu, l-Ser and l-Lys.
31976851	18	51	theme	pachnodae	2073:2081	arg1	comb					2083:2086	pachnodae comb	2073:2086	pachnodae comb	2073:2086	nov. and Xylanimonas pachnodae comb.
31976851	5	52	contain	had	577:579	arg1	2JSPR-7T					568:575	2JSPR-7T	568:575	2JSPR-7T	568:575	2JSPR-7T had the highest 16S rRNA gene sequence similarity to Xylanibacterium ulmi XIL08T (98.1 %), Xylanimicrobium pachnodae NBRC 107786T (97.8 %) and Xylanimonas cellulosilytica DSM 15894T (97.5 %).
31976851	5	52	contain	had	577:579	arg2	similarity					616:625	the highest 16S rRNA gene sequence similarity	581:625	the highest 16S rRNA gene sequence similarity to Xylanibacterium ulmi XIL08T (98.1 %), Xylanimicrobium pachnodae NBRC 107786T (97.8 %) and Xylanimonas cellulosilytica DSM 15894T (97.5 %)	581:766	2JSPR-7T had the highest 16S rRNA gene sequence similarity to Xylanibacterium ulmi XIL08T (98.1 %), Xylanimicrobium pachnodae NBRC 107786T (97.8 %) and Xylanimonas cellulosilytica DSM 15894T (97.5 %).
31976851	14	53	theme	allomyrinae	1789:1799	arg1	sp					1801:1802	Xylanimonas allomyrinae sp	1777:1802	Xylanimonas allomyrinae sp	1777:1802	Therefore, it is proposed to combine the genera Xylanimicrobium and Xylanibacterium with the genus Xylanimonas considering the priority of publication and to classify strain 2JSPR-7T in the genus as Xylanimonas allomyrinae sp.
31976851	4	54	theme	dichotoma	458:466	arg1	larvae					437:442	larvae	437:442	larvae of Allomyrina dichotoma	437:466	A bacterium that was Gram-staining-positive, facultatively anaerobic, non-motile, rod- or filamentous-shaped, designated as strain 2JSPR-7T, was isolated from a gut of larvae of Allomyrina dichotoma which were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
31976851	10	55	theme	type	1153:1156	arg1	structure					1119:1127	The peptidoglycan structure	1101:1127	The peptidoglycan structure	1101:1127	The peptidoglycan structure was suggested to be the type A3α (A11.14) l-Lys-l-Ser with the presence of d-Ala, l-Ala, d-Glu, l-Ser and l-Lys.
31976851	10	55	theme	type	1153:1156	arg1	l-Lys-l-Ser					1171:1181	the type A3α (A11.14) l-Lys-l-Ser	1149:1181	the type A3α (A11.14) l-Lys-l-Ser with the presence of d-Ala, l-Ala, d-Glu, l-Ser and l-Lys	1149:1239	The peptidoglycan structure was suggested to be the type A3α (A11.14) l-Lys-l-Ser with the presence of d-Ala, l-Ala, d-Glu, l-Ser and l-Lys.
31976851	1	56	theme	ulmi	125:128	arg1	larva					58:62	larva	58:62	larva of Allomyrina dichotoma	58:86	nov. isolated from the gut of larva of Allomyrina dichotoma, reclassification of Xylanibacterium ulmi as Xylanimonas ulmi comb.
31976851	1	56	theme	ulmi	125:128	arg1	reclassification					89:104	reclassification	89:104	reclassification of Xylanibacterium ulmi as Xylanimonas ulmi comb	89:153	nov. isolated from the gut of larva of Allomyrina dichotoma, reclassification of Xylanibacterium ulmi as Xylanimonas ulmi comb.
31976851	5	57	theme	Xylanimonas	720:730	arg1	%					765:765	97.5 %	760:765	97.5 %	760:765	2JSPR-7T had the highest 16S rRNA gene sequence similarity to Xylanibacterium ulmi XIL08T (98.1 %), Xylanimicrobium pachnodae NBRC 107786T (97.8 %) and Xylanimonas cellulosilytica DSM 15894T (97.5 %).
31976851	5	57	theme	Xylanimonas	720:730	arg1	15894T					752:757	Xylanimonas cellulosilytica DSM 15894T	720:757	Xylanimonas cellulosilytica DSM 15894T (97.5 %)	720:766	2JSPR-7T had the highest 16S rRNA gene sequence similarity to Xylanibacterium ulmi XIL08T (98.1 %), Xylanimicrobium pachnodae NBRC 107786T (97.8 %) and Xylanimonas cellulosilytica DSM 15894T (97.5 %).
31976851	1	58	theme	Xylanimonas	133:143	arg1	comb					150:153	Xylanimonas ulmi comb	133:153	Xylanimonas ulmi comb	133:153	nov. isolated from the gut of larva of Allomyrina dichotoma, reclassification of Xylanibacterium ulmi as Xylanimonas ulmi comb.
31976851	4	59	theme	National	493:500	arg1	Institute					502:510	the National Institute	489:510	the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea	489:565	A bacterium that was Gram-staining-positive, facultatively anaerobic, non-motile, rod- or filamentous-shaped, designated as strain 2JSPR-7T, was isolated from a gut of larvae of Allomyrina dichotoma which were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
31976851	17	60	theme	Xylanimonas	1930:1940	arg1	description					1905:1915	the description	1901:1915	the description of the genus Xylanimonas	1901:1940	In addition, the description of the genus Xylanimonas is emended, and Xylanibacterium ulmi and Xylanimicrobium pachnodae are reclassified as Xylanimonas ulmi comb.
31976851	5	61	theme	DSM	748:750	arg1	%					765:765	97.5 %	760:765	97.5 %	760:765	2JSPR-7T had the highest 16S rRNA gene sequence similarity to Xylanibacterium ulmi XIL08T (98.1 %), Xylanimicrobium pachnodae NBRC 107786T (97.8 %) and Xylanimonas cellulosilytica DSM 15894T (97.5 %).
31976851	5	61	theme	DSM	748:750	arg1	15894T					752:757	Xylanimonas cellulosilytica DSM 15894T	720:757	Xylanimonas cellulosilytica DSM 15894T (97.5 %)	720:766	2JSPR-7T had the highest 16S rRNA gene sequence similarity to Xylanibacterium ulmi XIL08T (98.1 %), Xylanimicrobium pachnodae NBRC 107786T (97.8 %) and Xylanimonas cellulosilytica DSM 15894T (97.5 %).
31976851	5	62	theme	16S	593:595	arg1	similarity					616:625	the highest 16S rRNA gene sequence similarity	581:625	the highest 16S rRNA gene sequence similarity to Xylanibacterium ulmi XIL08T (98.1 %), Xylanimicrobium pachnodae NBRC 107786T (97.8 %) and Xylanimonas cellulosilytica DSM 15894T (97.5 %)	581:766	2JSPR-7T had the highest 16S rRNA gene sequence similarity to Xylanibacterium ulmi XIL08T (98.1 %), Xylanimicrobium pachnodae NBRC 107786T (97.8 %) and Xylanimonas cellulosilytica DSM 15894T (97.5 %).
31976851	16	63	theme	19330T=NBRC	1866:1876	arg1	113052T					1878:1884	=KACC 19330T=NBRC 113052T	1860:1884	=KACC 19330T=NBRC 113052T	1860:1884	The type strain of the novel species is 2JSPR-7T (=KACC 19330T=NBRC 113052T).
31976851	16	63	theme	19330T=NBRC	1866:1876	arg1	2JSPR-7T					1850:1857	2JSPR-7T	1850:1857	2JSPR-7T (=KACC 19330T=NBRC 113052T)	1850:1885	The type strain of the novel species is 2JSPR-7T (=KACC 19330T=NBRC 113052T).
31976851	5	64	dep	Xylanimicrobium	668:682	arg1	pachnodae					684:692	pachnodae	684:692	pachnodae	684:692	2JSPR-7T had the highest 16S rRNA gene sequence similarity to Xylanibacterium ulmi XIL08T (98.1 %), Xylanimicrobium pachnodae NBRC 107786T (97.8 %) and Xylanimonas cellulosilytica DSM 15894T (97.5 %).
31976851	5	65	theme	gene	602:605	arg1	similarity					616:625	the highest 16S rRNA gene sequence similarity	581:625	the highest 16S rRNA gene sequence similarity to Xylanibacterium ulmi XIL08T (98.1 %), Xylanimicrobium pachnodae NBRC 107786T (97.8 %) and Xylanimonas cellulosilytica DSM 15894T (97.5 %)	581:766	2JSPR-7T had the highest 16S rRNA gene sequence similarity to Xylanibacterium ulmi XIL08T (98.1 %), Xylanimicrobium pachnodae NBRC 107786T (97.8 %) and Xylanimonas cellulosilytica DSM 15894T (97.5 %).
31976851	1	66	theme	larva	58:62	arg1	gut					51:53	the gut	47:53	the gut of larva of Allomyrina dichotoma, reclassification of Xylanibacterium ulmi as Xylanimonas ulmi comb	47:153	nov. isolated from the gut of larva of Allomyrina dichotoma, reclassification of Xylanibacterium ulmi as Xylanimonas ulmi comb.
31976851	0	67	theme	allomyrinae	12:22	arg1	sp					24:25	Xylanimonas allomyrinae sp	0:25	Xylanimonas allomyrinae sp.	0:26	Xylanimonas allomyrinae sp.
31976851	1	68	theme	Allomyrina	67:76	arg1	dichotoma					78:86	Allomyrina dichotoma	67:86	Allomyrina dichotoma	67:86	nov. isolated from the gut of larva of Allomyrina dichotoma, reclassification of Xylanibacterium ulmi as Xylanimonas ulmi comb.
31976851	5	69	dep	Xylanimonas	720:730	arg1	cellulosilytica					732:746	cellulosilytica	732:746	cellulosilytica	732:746	2JSPR-7T had the highest 16S rRNA gene sequence similarity to Xylanibacterium ulmi XIL08T (98.1 %), Xylanimicrobium pachnodae NBRC 107786T (97.8 %) and Xylanimonas cellulosilytica DSM 15894T (97.5 %).
31976851	4	70	theme	Republic	549:556	arg1	Institute					502:510	the National Institute	489:510	the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea	489:565	A bacterium that was Gram-staining-positive, facultatively anaerobic, non-motile, rod- or filamentous-shaped, designated as strain 2JSPR-7T, was isolated from a gut of larvae of Allomyrina dichotoma which were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
31976851	10	71	theme	l-Ala	1211:1215	arg1	presence					1192:1199	the presence	1188:1199	the presence of d-Ala, l-Ala, d-Glu, l-Ser and l-Lys	1188:1239	The peptidoglycan structure was suggested to be the type A3α (A11.14) l-Lys-l-Ser with the presence of d-Ala, l-Ala, d-Glu, l-Ser and l-Lys.
31976851	13	72	theme	suitable	1371:1378	arg1	genus					1380:1384	a suitable genus	1369:1384	a suitable genus to accommodate strain 2JSPR-7T from any of the genera Xylanimonas, Xylanimicrobium and Xylanibacterium based on the polyphasic approach including phylogenetic and phenotypic characterization	1369:1575	We encountered difficulty in selecting a suitable genus to accommodate strain 2JSPR-7T from any of the genera Xylanimonas, Xylanimicrobium and Xylanibacterium based on the polyphasic approach including phylogenetic and phenotypic characterization.
31976851	14	73	theme	publication	1717:1727	arg1	priority					1705:1712	the priority	1701:1712	the priority of publication	1701:1727	Therefore, it is proposed to combine the genera Xylanimicrobium and Xylanibacterium with the genus Xylanimonas considering the priority of publication and to classify strain 2JSPR-7T in the genus as Xylanimonas allomyrinae sp.
31976851	4	74	attach	isolated	414:421	arg1	gut					430:432	a gut	428:432	a gut of larvae of Allomyrina dichotoma which were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea	428:565	A bacterium that was Gram-staining-positive, facultatively anaerobic, non-motile, rod- or filamentous-shaped, designated as strain 2JSPR-7T, was isolated from a gut of larvae of Allomyrina dichotoma which were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
31976851	4	74	attach	isolated	414:421	arg2	bacterium					271:279	A bacterium that was Gram-staining-positive, facultatively anaerobic, non-motile, rod- or filamentous-shaped, designated as strain 2JSPR-7T,	269:408	bacterium	271:279	A bacterium that was Gram-staining-positive, facultatively anaerobic, non-motile, rod- or filamentous-shaped, designated as strain 2JSPR-7T, was isolated from a gut of larvae of Allomyrina dichotoma which were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
31976851	4	75	theme	Korea	561:565	arg1	Institute					502:510	the National Institute	489:510	the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea	489:565	A bacterium that was Gram-staining-positive, facultatively anaerobic, non-motile, rod- or filamentous-shaped, designated as strain 2JSPR-7T, was isolated from a gut of larvae of Allomyrina dichotoma which were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
31976851	10	76	theme	d-Ala	1204:1208	arg1	presence					1192:1199	the presence	1188:1199	the presence of d-Ala, l-Ala, d-Glu, l-Ser and l-Lys	1188:1239	The peptidoglycan structure was suggested to be the type A3α (A11.14) l-Lys-l-Ser with the presence of d-Ala, l-Ala, d-Glu, l-Ser and l-Lys.
31976851	16	77	theme	type	1814:1817	arg1	strain					1819:1824	The type strain	1810:1824	The type strain of the novel species	1810:1845	The type strain of the novel species is 2JSPR-7T (=KACC 19330T=NBRC 113052T).
31976851	16	77	theme	type	1814:1817	arg1	2JSPR-7T					1850:1857	2JSPR-7T	1850:1857	2JSPR-7T (=KACC 19330T=NBRC 113052T)	1850:1885	The type strain of the novel species is 2JSPR-7T (=KACC 19330T=NBRC 113052T).
31976851	13	78	theme	phenotypic	1549:1558	arg1	characterization					1560:1575	phenotypic characterization	1549:1575	phenotypic characterization	1549:1575	We encountered difficulty in selecting a suitable genus to accommodate strain 2JSPR-7T from any of the genera Xylanimonas, Xylanimicrobium and Xylanibacterium based on the polyphasic approach including phylogenetic and phenotypic characterization.
31976851	10	79	theme	l-Ser	1225:1229	arg1	presence					1192:1199	the presence	1188:1199	the presence of d-Ala, l-Ala, d-Glu, l-Ser and l-Lys	1188:1239	The peptidoglycan structure was suggested to be the type A3α (A11.14) l-Lys-l-Ser with the presence of d-Ala, l-Ala, d-Glu, l-Ser and l-Lys.
31976851	16	80	theme	novel	1833:1837	arg1	species					1839:1845	the novel species	1829:1845	the novel species	1829:1845	The type strain of the novel species is 2JSPR-7T (=KACC 19330T=NBRC 113052T).
31976851	17	81	theme	ulmi	2041:2044	arg1	ulmi					1974:1977	ulmi	1974:1977	ulmi	1974:1977	In addition, the description of the genus Xylanimonas is emended, and Xylanibacterium ulmi and Xylanimicrobium pachnodae are reclassified as Xylanimonas ulmi comb.
31976851	17	81	theme	ulmi	2041:2044	arg1	pachnodae					1999:2007	pachnodae	1999:2007	pachnodae	1999:2007	In addition, the description of the genus Xylanimonas is emended, and Xylanibacterium ulmi and Xylanimicrobium pachnodae are reclassified as Xylanimonas ulmi comb.
31976851	17	81	theme	ulmi	2041:2044	arg1	comb					2046:2049	Xylanimonas ulmi comb	2029:2049	Xylanimonas ulmi comb	2029:2049	In addition, the description of the genus Xylanimonas is emended, and Xylanibacterium ulmi and Xylanimicrobium pachnodae are reclassified as Xylanimonas ulmi comb.
31976851	10	82	theme	d-Glu	1218:1222	arg1	presence					1192:1199	the presence	1188:1199	the presence of d-Ala, l-Ala, d-Glu, l-Ser and l-Lys	1188:1239	The peptidoglycan structure was suggested to be the type A3α (A11.14) l-Lys-l-Ser with the presence of d-Ala, l-Ala, d-Glu, l-Ser and l-Lys.
31976851	7	83	theme	fatty	861:865	arg1	acids					867:871	The cellular fatty acids	848:871	The cellular fatty acids	848:871	The cellular fatty acids mainly consisted of anteiso-C15 : 0, C14 : 0 and C16 : 0.
31976851	14	84	theme	genus	1671:1675	arg1	Xylanimonas					1677:1687	the genus Xylanimonas	1667:1687	the genus Xylanimonas considering the priority of publication	1667:1727	Therefore, it is proposed to combine the genera Xylanimicrobium and Xylanibacterium with the genus Xylanimonas considering the priority of publication and to classify strain 2JSPR-7T in the genus as Xylanimonas allomyrinae sp.
31976851	2	85	theme	Xylanimonas	194:204	arg1	comb					216:219	Xylanimonas pachnodae comb	194:219	Xylanimonas pachnodae comb	194:219	nov. and Xylanimicrobium pachnodae as Xylanimonas pachnodae comb.
31976851	10	86	theme	l-Lys	1235:1239	arg1	presence					1192:1199	the presence	1188:1199	the presence of d-Ala, l-Ala, d-Glu, l-Ser and l-Lys	1188:1239	The peptidoglycan structure was suggested to be the type A3α (A11.14) l-Lys-l-Ser with the presence of d-Ala, l-Ala, d-Glu, l-Ser and l-Lys.
33937995	10	0	theme	Essential	1498:1506	arg1	oil-					1508:1511	Essential oil-	1498:1511	Essential oil-	1498:1511	Essential oil- and CS-based coating can be used as alternative to synthetic preservatives, which are associated with various health hazards.
33937995	10	0	theme	Essential	1498:1506	arg1	preservatives					1574:1586	alternative to synthetic preservatives	1549:1586	alternative to synthetic preservatives	1549:1586	Essential oil- and CS-based coating can be used as alternative to synthetic preservatives, which are associated with various health hazards.
33937995	10	0	theme	Essential	1498:1506	arg1	coating					1526:1532	CS-based coating	1517:1532	CS-based coating	1517:1532	Essential oil- and CS-based coating can be used as alternative to synthetic preservatives, which are associated with various health hazards.
33937995	5	1	dep	transform	529:537	arg1	infrared					539:546	infrared	539:546	transform infrared spectroscopy (FTIR) and gas chromatography-mass spectrometry (GC-MS) analyses	529:624	Fourier transform infrared spectroscopy (FTIR) and gas chromatography-mass spectrometry (GC-MS) analyses were performed to identify the chemical composition of the EO.
33937995	8	2	theme	hyphal	1071:1076	arg1	degradation					1078:1088	hyphal degradation	1071:1088	hyphal degradation	1071:1088	Essential oils extracted by ethanol from dried peels showed complete inhibition of A. niger and P. citrinum and hyphal degradation at a minimum inhibitory concentration (MIC) of 100 μL mL-1 .
33937995	4	3	theme	orange	454:459	arg1	peels					461:465	dried and fresh sweet orange peels	432:465	dried and fresh sweet orange peels	432:465	Essential oils were extracted from dried and fresh sweet orange peels by using n-hexane and ethanol as extraction solvents.
33937995	8	4	theme	Essential	959:967	arg1	oils					969:972	Essential oils	959:972	Essential oils extracted by ethanol from dried peels	959:1010	Essential oils extracted by ethanol from dried peels showed complete inhibition of A. niger and P. citrinum and hyphal degradation at a minimum inhibitory concentration (MIC) of 100 μL mL-1 .
33937995	1	5	theme	BACKGROUND	131:140	arg1	cause					174:178	a major cause	166:178	a major cause of food spoilage	166:195	BACKGROUND Fungal contamination is a major cause of food spoilage.
33937995	1	5	theme	BACKGROUND	131:140	arg1	contamination					149:161	BACKGROUND Fungal contamination	131:161	BACKGROUND Fungal contamination	131:161	BACKGROUND Fungal contamination is a major cause of food spoilage.
33937995	7	6	theme	spp	954:956	arg1	prevalence					912:921	a high prevalence	905:921	a high prevalence of Aspergillus and Penicillium spp	905:956	RESULTS Tomatoes obtained from local markets and supermarkets showed a high prevalence of Aspergillus and Penicillium spp.
33937995	0	7	from	Prevalence	0:9	arg1	tomatoes					29:36	fresh tomatoes	23:36	fresh tomatoes	23:36	Prevalence of fungi in fresh tomatoes and their control by chitosan and sweet orange (Citrus sinensis) peel essential oil coating.
33937995	2	8	theme	natural	247:253	arg1	preservatives					255:267	natural preservatives	247:267	natural preservatives	247:267	There is an urgent need to find and characterize natural preservatives.
33937995	10	9	dep	preservatives	1574:1586	arg1	to					1561:1562	to	1561:1562	to	1561:1562	Essential oil- and CS-based coating can be used as alternative to synthetic preservatives, which are associated with various health hazards.
33937995	0	10	theme	sweet	72:76	arg1	orange					78:83	sweet orange	72:83	sweet orange (Citrus sinensis)	72:101	Prevalence of fungi in fresh tomatoes and their control by chitosan and sweet orange (Citrus sinensis) peel essential oil coating.
33937995	0	10	theme	sweet	72:76	arg1	sinensis					93:100	Citrus sinensis	86:100	Citrus sinensis	86:100	Prevalence of fungi in fresh tomatoes and their control by chitosan and sweet orange (Citrus sinensis) peel essential oil coating.
33937995	6	11	theme	Penicillium	814:824	arg1	citrinum					826:833	Penicillium citrinum	814:833	Penicillium citrinum	814:833	A combination of chitosan (CS) and EO was used to control the fungal decay of tomatoes inoculated with Aspergillus niger and Penicillium citrinum.
33937995	7	12	dep	RESULTS	836:842	arg1	Tomatoes					844:851	Tomatoes	844:851	RESULTS Tomatoes obtained from local markets and supermarkets	836:896	RESULTS Tomatoes obtained from local markets and supermarkets showed a high prevalence of Aspergillus and Penicillium spp.
33937995	9	13	with	combination	1155:1165	arg1	chitosan					1178:1185	chitosan	1178:1185	chitosan (2%) as a coating, effectively controlled the fungal decay of tomatoes until the eighth day of storage at 25 °C. CONCLUSION Due to their edible nature, and their antifungal and preservative potential, EO- and CS-based coatings	1178:1412	The combination of EO with chitosan (2%) as a coating, effectively controlled the fungal decay of tomatoes until the eighth day of storage at 25 °C. CONCLUSION Due to their edible nature, and their antifungal and preservative potential, EO- and CS-based coatings can be used to extend the shelf life of tomatoes and other agriculture commodities.
33937995	9	13	with	combination	1155:1165	arg1	%					1189:1189	2%	1188:1189	2%	1188:1189	The combination of EO with chitosan (2%) as a coating, effectively controlled the fungal decay of tomatoes until the eighth day of storage at 25 °C. CONCLUSION Due to their edible nature, and their antifungal and preservative potential, EO- and CS-based coatings can be used to extend the shelf life of tomatoes and other agriculture commodities.
33937995	4	14	theme	Essential	397:405	arg1	oils					407:410	Essential oils	397:410	Essential oils	397:410	Essential oils were extracted from dried and fresh sweet orange peels by using n-hexane and ethanol as extraction solvents.
33937995	3	15	theme	fungi	309:313	arg1	prevalence					295:304	the prevalence	291:304	the prevalence of fungi in tomatoes and their control	291:343	This study evaluates the prevalence of fungi in tomatoes and their control by using essential oil (EO) from sweet orange peel.
33937995	8	16	theme	inhibitory	1103:1112	arg1	MIC					1129:1131	MIC	1129:1131	MIC	1129:1131	Essential oils extracted by ethanol from dried peels showed complete inhibition of A. niger and P. citrinum and hyphal degradation at a minimum inhibitory concentration (MIC) of 100 μL mL-1 .
33937995	8	16	theme	inhibitory	1103:1112	arg1	concentration					1114:1126	a minimum inhibitory concentration	1093:1126	a minimum inhibitory concentration (MIC) of 100 μL mL-1	1093:1147	Essential oils extracted by ethanol from dried peels showed complete inhibition of A. niger and P. citrinum and hyphal degradation at a minimum inhibitory concentration (MIC) of 100 μL mL-1 .
33937995	6	17	theme	EO	724:725	arg1	combination					691:701	A combination	689:701	A combination of chitosan (CS) and EO	689:725	A combination of chitosan (CS) and EO was used to control the fungal decay of tomatoes inoculated with Aspergillus niger and Penicillium citrinum.
33937995	1	18	theme	Fungal	142:147	arg1	cause					174:178	a major cause	166:178	a major cause of food spoilage	166:195	BACKGROUND Fungal contamination is a major cause of food spoilage.
33937995	1	18	theme	Fungal	142:147	arg1	contamination					149:161	BACKGROUND Fungal contamination	131:161	BACKGROUND Fungal contamination	131:161	BACKGROUND Fungal contamination is a major cause of food spoilage.
33937995	8	19	theme	minimum	1095:1101	arg1	MIC					1129:1131	MIC	1129:1131	MIC	1129:1131	Essential oils extracted by ethanol from dried peels showed complete inhibition of A. niger and P. citrinum and hyphal degradation at a minimum inhibitory concentration (MIC) of 100 μL mL-1 .
33937995	8	19	theme	minimum	1095:1101	arg1	concentration					1114:1126	a minimum inhibitory concentration	1093:1126	a minimum inhibitory concentration (MIC) of 100 μL mL-1	1093:1147	Essential oils extracted by ethanol from dried peels showed complete inhibition of A. niger and P. citrinum and hyphal degradation at a minimum inhibitory concentration (MIC) of 100 μL mL-1 .
33937995	4	20	theme	dried	432:436	arg1	peels					461:465	dried and fresh sweet orange peels	432:465	dried and fresh sweet orange peels	432:465	Essential oils were extracted from dried and fresh sweet orange peels by using n-hexane and ethanol as extraction solvents.
33937995	7	21	theme	local	867:871	arg1	markets					873:879	local markets	867:879	local markets	867:879	RESULTS Tomatoes obtained from local markets and supermarkets showed a high prevalence of Aspergillus and Penicillium spp.
33937995	10	22	theme	health	1623:1628	arg1	hazards					1630:1636	various health hazards	1615:1636	various health hazards	1615:1636	Essential oil- and CS-based coating can be used as alternative to synthetic preservatives, which are associated with various health hazards.
33937995	11	23	theme	Chemical	1657:1664	arg1	Industry					1666:1673	Chemical Industry	1657:1673	Chemical Industry	1657:1673	© 2021 Society of Chemical Industry.
33937995	3	24	theme	orange	384:389	arg1	peel					391:394	sweet orange peel	378:394	sweet orange peel	378:394	This study evaluates the prevalence of fungi in tomatoes and their control by using essential oil (EO) from sweet orange peel.
33937995	6	25	theme	Aspergillus	792:802	arg1	niger					804:808	Aspergillus niger	792:808	Aspergillus niger	792:808	A combination of chitosan (CS) and EO was used to control the fungal decay of tomatoes inoculated with Aspergillus niger and Penicillium citrinum.
33937995	5	26	theme	gas	572:574	arg1	GC-MS					610:614	GC-MS	610:614	GC-MS	610:614	Fourier transform infrared spectroscopy (FTIR) and gas chromatography-mass spectrometry (GC-MS) analyses were performed to identify the chemical composition of the EO.
33937995	5	26	theme	gas	572:574	arg1	spectrometry					596:607	gas chromatography-mass spectrometry	572:607	gas chromatography-mass spectrometry (GC-MS)	572:615	Fourier transform infrared spectroscopy (FTIR) and gas chromatography-mass spectrometry (GC-MS) analyses were performed to identify the chemical composition of the EO.
33937995	9	27	theme	shelf	1440:1444	arg1	life					1446:1449	the shelf life	1436:1449	the shelf life of tomatoes and other agriculture commodities	1436:1495	The combination of EO with chitosan (2%) as a coating, effectively controlled the fungal decay of tomatoes until the eighth day of storage at 25 °C. CONCLUSION Due to their edible nature, and their antifungal and preservative potential, EO- and CS-based coatings can be used to extend the shelf life of tomatoes and other agriculture commodities.
33937995	10	28	used	used	1541:1544	arg2	oil-					1508:1511	Essential oil-	1498:1511	Essential oil-	1498:1511	Essential oil- and CS-based coating can be used as alternative to synthetic preservatives, which are associated with various health hazards.
33937995	10	28	used	used	1541:1544	arg2	preservatives					1574:1586	alternative to synthetic preservatives	1549:1586	alternative to synthetic preservatives	1549:1586	Essential oil- and CS-based coating can be used as alternative to synthetic preservatives, which are associated with various health hazards.
33937995	10	28	used	used	1541:1544	arg2	coating					1526:1532	CS-based coating	1517:1532	CS-based coating	1517:1532	Essential oil- and CS-based coating can be used as alternative to synthetic preservatives, which are associated with various health hazards.
33937995	5	29	theme	chemical	657:664	arg1	composition					666:676	the chemical composition	653:676	the chemical composition of the EO	653:686	Fourier transform infrared spectroscopy (FTIR) and gas chromatography-mass spectrometry (GC-MS) analyses were performed to identify the chemical composition of the EO.
33937995	9	30	theme	fungal	1233:1238	arg1	decay					1240:1244	the fungal decay	1229:1244	the fungal decay of tomatoes	1229:1256	The combination of EO with chitosan (2%) as a coating, effectively controlled the fungal decay of tomatoes until the eighth day of storage at 25 °C. CONCLUSION Due to their edible nature, and their antifungal and preservative potential, EO- and CS-based coatings can be used to extend the shelf life of tomatoes and other agriculture commodities.
33937995	5	31	theme	chromatography-mass	576:594	arg1	GC-MS					610:614	GC-MS	610:614	GC-MS	610:614	Fourier transform infrared spectroscopy (FTIR) and gas chromatography-mass spectrometry (GC-MS) analyses were performed to identify the chemical composition of the EO.
33937995	5	31	theme	chromatography-mass	576:594	arg1	spectrometry					596:607	gas chromatography-mass spectrometry	572:607	gas chromatography-mass spectrometry (GC-MS)	572:615	Fourier transform infrared spectroscopy (FTIR) and gas chromatography-mass spectrometry (GC-MS) analyses were performed to identify the chemical composition of the EO.
33937995	9	32	from	day	1275:1277	arg1	CONCLUSION					1300:1309	25 °C. CONCLUSION	1293:1309	25 °C. CONCLUSION	1293:1309	The combination of EO with chitosan (2%) as a coating, effectively controlled the fungal decay of tomatoes until the eighth day of storage at 25 °C. CONCLUSION Due to their edible nature, and their antifungal and preservative potential, EO- and CS-based coatings can be used to extend the shelf life of tomatoes and other agriculture commodities.
33937995	8	33	theme	complete	1019:1026	arg1	inhibition					1028:1037	complete inhibition	1019:1037	complete inhibition of A. niger and P. citrinum	1019:1065	Essential oils extracted by ethanol from dried peels showed complete inhibition of A. niger and P. citrinum and hyphal degradation at a minimum inhibitory concentration (MIC) of 100 μL mL-1 .
33937995	9	34	used	used	1421:1424	arg2	combination					1155:1165	The combination	1151:1165	The combination of EO with chitosan (2%) as a coating, effectively controlled the fungal decay of tomatoes until the eighth day of storage at 25 °C. CONCLUSION Due to their edible nature, and their antifungal and preservative potential, EO- and CS-based coatings	1151:1412	The combination of EO with chitosan (2%) as a coating, effectively controlled the fungal decay of tomatoes until the eighth day of storage at 25 °C. CONCLUSION Due to their edible nature, and their antifungal and preservative potential, EO- and CS-based coatings can be used to extend the shelf life of tomatoes and other agriculture commodities.
33937995	1	35	theme	major	168:172	arg1	cause					174:178	a major cause	166:178	a major cause of food spoilage	166:195	BACKGROUND Fungal contamination is a major cause of food spoilage.
33937995	1	35	theme	major	168:172	arg1	contamination					149:161	BACKGROUND Fungal contamination	131:161	BACKGROUND Fungal contamination	131:161	BACKGROUND Fungal contamination is a major cause of food spoilage.
33937995	0	36	theme	fungi	14:18	arg1	Prevalence					0:9	Prevalence	0:9	Prevalence of fungi in fresh tomatoes	0:36	Prevalence of fungi in fresh tomatoes and their control by chitosan and sweet orange (Citrus sinensis) peel essential oil coating.
33937995	0	36	theme	fungi	14:18	arg1	control					48:54	their control	42:54	their control by chitosan and sweet orange (Citrus sinensis)	42:101	Prevalence of fungi in fresh tomatoes and their control by chitosan and sweet orange (Citrus sinensis) peel essential oil coating.
33937995	4	37	theme	extraction	500:509	arg1	ethanol					489:495	ethanol	489:495	ethanol	489:495	Essential oils were extracted from dried and fresh sweet orange peels by using n-hexane and ethanol as extraction solvents.
33937995	4	37	theme	extraction	500:509	arg1	solvents					511:518	extraction solvents	500:518	extraction solvents	500:518	Essential oils were extracted from dried and fresh sweet orange peels by using n-hexane and ethanol as extraction solvents.
33937995	4	37	theme	extraction	500:509	arg1	n-hexane					476:483	n-hexane	476:483	n-hexane	476:483	Essential oils were extracted from dried and fresh sweet orange peels by using n-hexane and ethanol as extraction solvents.
33937995	10	38	theme	various	1615:1621	arg1	hazards					1630:1636	various health hazards	1615:1636	various health hazards	1615:1636	Essential oil- and CS-based coating can be used as alternative to synthetic preservatives, which are associated with various health hazards.
33937995	9	39	theme	tomatoes	1454:1461	arg1	life					1446:1449	the shelf life	1436:1449	the shelf life of tomatoes and other agriculture commodities	1436:1495	The combination of EO with chitosan (2%) as a coating, effectively controlled the fungal decay of tomatoes until the eighth day of storage at 25 °C. CONCLUSION Due to their edible nature, and their antifungal and preservative potential, EO- and CS-based coatings can be used to extend the shelf life of tomatoes and other agriculture commodities.
33937995	9	40	from	CONCLUSION	1300:1309	arg1	storage					1282:1288	storage	1282:1288	storage at 25 °C. CONCLUSION Due to their edible nature	1282:1336	The combination of EO with chitosan (2%) as a coating, effectively controlled the fungal decay of tomatoes until the eighth day of storage at 25 °C. CONCLUSION Due to their edible nature, and their antifungal and preservative potential, EO- and CS-based coatings can be used to extend the shelf life of tomatoes and other agriculture commodities.
33937995	9	40	from	CONCLUSION	1300:1309	arg1	day					1275:1277	the eighth day	1264:1277	the eighth day of storage at 25 °C. CONCLUSION Due to their edible nature	1264:1336	The combination of EO with chitosan (2%) as a coating, effectively controlled the fungal decay of tomatoes until the eighth day of storage at 25 °C. CONCLUSION Due to their edible nature, and their antifungal and preservative potential, EO- and CS-based coatings can be used to extend the shelf life of tomatoes and other agriculture commodities.
33937995	10	41	theme	alternative	1549:1559	arg1	oil-					1508:1511	Essential oil-	1498:1511	Essential oil-	1498:1511	Essential oil- and CS-based coating can be used as alternative to synthetic preservatives, which are associated with various health hazards.
33937995	10	41	theme	alternative	1549:1559	arg1	preservatives					1574:1586	alternative to synthetic preservatives	1549:1586	alternative to synthetic preservatives	1549:1586	Essential oil- and CS-based coating can be used as alternative to synthetic preservatives, which are associated with various health hazards.
33937995	10	41	theme	alternative	1549:1559	arg1	coating					1526:1532	CS-based coating	1517:1532	CS-based coating	1517:1532	Essential oil- and CS-based coating can be used as alternative to synthetic preservatives, which are associated with various health hazards.
33937995	8	42	theme	100 μL mL-1	1137:1147	arg1	MIC					1129:1131	MIC	1129:1131	MIC	1129:1131	Essential oils extracted by ethanol from dried peels showed complete inhibition of A. niger and P. citrinum and hyphal degradation at a minimum inhibitory concentration (MIC) of 100 μL mL-1 .
33937995	8	42	theme	100 μL mL-1	1137:1147	arg1	concentration					1114:1126	a minimum inhibitory concentration	1093:1126	a minimum inhibitory concentration (MIC) of 100 μL mL-1	1093:1147	Essential oils extracted by ethanol from dried peels showed complete inhibition of A. niger and P. citrinum and hyphal degradation at a minimum inhibitory concentration (MIC) of 100 μL mL-1 .
33937995	9	43	theme	tomatoes	1249:1256	arg1	decay					1240:1244	the fungal decay	1229:1244	the fungal decay of tomatoes	1229:1256	The combination of EO with chitosan (2%) as a coating, effectively controlled the fungal decay of tomatoes until the eighth day of storage at 25 °C. CONCLUSION Due to their edible nature, and their antifungal and preservative potential, EO- and CS-based coatings can be used to extend the shelf life of tomatoes and other agriculture commodities.
33937995	0	44	theme	essential	108:116	arg1	coating					122:128	essential oil coating	108:128	essential oil coating	108:128	Prevalence of fungi in fresh tomatoes and their control by chitosan and sweet orange (Citrus sinensis) peel essential oil coating.
33937995	0	45	theme	Citrus	86:91	arg1	orange					78:83	sweet orange	72:83	sweet orange (Citrus sinensis)	72:101	Prevalence of fungi in fresh tomatoes and their control by chitosan and sweet orange (Citrus sinensis) peel essential oil coating.
33937995	0	45	theme	Citrus	86:91	arg1	sinensis					93:100	Citrus sinensis	86:100	Citrus sinensis	86:100	Prevalence of fungi in fresh tomatoes and their control by chitosan and sweet orange (Citrus sinensis) peel essential oil coating.
33937995	10	46	theme	CS-based	1517:1524	arg1	oil-					1508:1511	Essential oil-	1498:1511	Essential oil-	1498:1511	Essential oil- and CS-based coating can be used as alternative to synthetic preservatives, which are associated with various health hazards.
33937995	10	46	theme	CS-based	1517:1524	arg1	coating					1526:1532	CS-based coating	1517:1532	CS-based coating	1517:1532	Essential oil- and CS-based coating can be used as alternative to synthetic preservatives, which are associated with various health hazards.
33937995	10	46	theme	CS-based	1517:1524	arg1	preservatives					1574:1586	alternative to synthetic preservatives	1549:1586	alternative to synthetic preservatives	1549:1586	Essential oil- and CS-based coating can be used as alternative to synthetic preservatives, which are associated with various health hazards.
33937995	6	47	used	used	731:734	arg2	combination					691:701	A combination	689:701	A combination of chitosan (CS) and EO	689:725	A combination of chitosan (CS) and EO was used to control the fungal decay of tomatoes inoculated with Aspergillus niger and Penicillium citrinum.
33937995	3	48	theme	sweet	378:382	arg1	orange					384:389	sweet orange	378:389	sweet orange peel	378:394	This study evaluates the prevalence of fungi in tomatoes and their control by using essential oil (EO) from sweet orange peel.
33937995	0	49	theme	fresh	23:27	arg1	tomatoes					29:36	fresh tomatoes	23:36	fresh tomatoes	23:36	Prevalence of fungi in fresh tomatoes and their control by chitosan and sweet orange (Citrus sinensis) peel essential oil coating.
33937995	6	50	theme	tomatoes	767:774	arg1	decay					758:762	the fungal decay	747:762	the fungal decay of tomatoes inoculated with Aspergillus niger and Penicillium citrinum	747:833	A combination of chitosan (CS) and EO was used to control the fungal decay of tomatoes inoculated with Aspergillus niger and Penicillium citrinum.
33937995	9	51	theme	other	1467:1471	arg1	commodities					1485:1495	other agriculture commodities	1467:1495	other agriculture commodities	1467:1495	The combination of EO with chitosan (2%) as a coating, effectively controlled the fungal decay of tomatoes until the eighth day of storage at 25 °C. CONCLUSION Due to their edible nature, and their antifungal and preservative potential, EO- and CS-based coatings can be used to extend the shelf life of tomatoes and other agriculture commodities.
33937995	3	52	from	peel	391:394	arg1	oil					364:366	essential oil	354:366	essential oil (EO) from sweet orange peel	354:394	This study evaluates the prevalence of fungi in tomatoes and their control by using essential oil (EO) from sweet orange peel.
33937995	3	52	from	peel	391:394	arg1	EO					369:370	EO	369:370	EO	369:370	This study evaluates the prevalence of fungi in tomatoes and their control by using essential oil (EO) from sweet orange peel.
33937995	1	53	theme	food	183:186	arg1	spoilage					188:195	food spoilage	183:195	food spoilage	183:195	BACKGROUND Fungal contamination is a major cause of food spoilage.
33937995	3	54	theme	essential	354:362	arg1	oil					364:366	essential oil	354:366	essential oil (EO) from sweet orange peel	354:394	This study evaluates the prevalence of fungi in tomatoes and their control by using essential oil (EO) from sweet orange peel.
33937995	3	54	theme	essential	354:362	arg1	EO					369:370	EO	369:370	EO	369:370	This study evaluates the prevalence of fungi in tomatoes and their control by using essential oil (EO) from sweet orange peel.
33937995	8	55	theme	niger	1045:1049	arg1	inhibition					1028:1037	complete inhibition	1019:1037	complete inhibition of A. niger and P. citrinum	1019:1065	Essential oils extracted by ethanol from dried peels showed complete inhibition of A. niger and P. citrinum and hyphal degradation at a minimum inhibitory concentration (MIC) of 100 μL mL-1 .
33937995	8	55	theme	niger	1045:1049	arg1	degradation					1078:1088	hyphal degradation	1071:1088	hyphal degradation	1071:1088	Essential oils extracted by ethanol from dried peels showed complete inhibition of A. niger and P. citrinum and hyphal degradation at a minimum inhibitory concentration (MIC) of 100 μL mL-1 .
33937995	0	56	from	control	48:54	arg1	tomatoes					29:36	fresh tomatoes	23:36	fresh tomatoes	23:36	Prevalence of fungi in fresh tomatoes and their control by chitosan and sweet orange (Citrus sinensis) peel essential oil coating.
33937995	9	57	theme	agriculture	1473:1483	arg1	commodities					1485:1495	other agriculture commodities	1467:1495	other agriculture commodities	1467:1495	The combination of EO with chitosan (2%) as a coating, effectively controlled the fungal decay of tomatoes until the eighth day of storage at 25 °C. CONCLUSION Due to their edible nature, and their antifungal and preservative potential, EO- and CS-based coatings can be used to extend the shelf life of tomatoes and other agriculture commodities.
33937995	9	58	theme	edible	1324:1329	arg1	nature					1331:1336	their edible nature	1318:1336	their edible nature	1318:1336	The combination of EO with chitosan (2%) as a coating, effectively controlled the fungal decay of tomatoes until the eighth day of storage at 25 °C. CONCLUSION Due to their edible nature, and their antifungal and preservative potential, EO- and CS-based coatings can be used to extend the shelf life of tomatoes and other agriculture commodities.
33937995	9	59	theme	potential	1377:1385	arg1	coatings					1405:1412	preservative potential, EO- and CS-based coatings	1364:1412	preservative potential, EO- and CS-based coatings	1364:1412	The combination of EO with chitosan (2%) as a coating, effectively controlled the fungal decay of tomatoes until the eighth day of storage at 25 °C. CONCLUSION Due to their edible nature, and their antifungal and preservative potential, EO- and CS-based coatings can be used to extend the shelf life of tomatoes and other agriculture commodities.
33937995	4	60	theme	sweet	448:452	arg1	orange					454:459	sweet orange	448:459	dried and fresh sweet orange peels	432:465	Essential oils were extracted from dried and fresh sweet orange peels by using n-hexane and ethanol as extraction solvents.
33937995	9	61	theme	eighth	1268:1273	arg1	day					1275:1277	the eighth day	1264:1277	the eighth day of storage at 25 °C. CONCLUSION Due to their edible nature	1264:1336	The combination of EO with chitosan (2%) as a coating, effectively controlled the fungal decay of tomatoes until the eighth day of storage at 25 °C. CONCLUSION Due to their edible nature, and their antifungal and preservative potential, EO- and CS-based coatings can be used to extend the shelf life of tomatoes and other agriculture commodities.
33937995	0	62	theme	oil	118:120	arg1	coating					122:128	essential oil coating	108:128	essential oil coating	108:128	Prevalence of fungi in fresh tomatoes and their control by chitosan and sweet orange (Citrus sinensis) peel essential oil coating.
33937995	9	63	theme	commodities	1485:1495	arg1	life					1446:1449	the shelf life	1436:1449	the shelf life of tomatoes and other agriculture commodities	1436:1495	The combination of EO with chitosan (2%) as a coating, effectively controlled the fungal decay of tomatoes until the eighth day of storage at 25 °C. CONCLUSION Due to their edible nature, and their antifungal and preservative potential, EO- and CS-based coatings can be used to extend the shelf life of tomatoes and other agriculture commodities.
33937995	9	64	theme	EO	1170:1171	arg1	combination					1155:1165	The combination	1151:1165	The combination of EO with chitosan (2%) as a coating, effectively controlled the fungal decay of tomatoes until the eighth day of storage at 25 °C. CONCLUSION Due to their edible nature, and their antifungal and preservative potential, EO- and CS-based coatings	1151:1412	The combination of EO with chitosan (2%) as a coating, effectively controlled the fungal decay of tomatoes until the eighth day of storage at 25 °C. CONCLUSION Due to their edible nature, and their antifungal and preservative potential, EO- and CS-based coatings can be used to extend the shelf life of tomatoes and other agriculture commodities.
33937995	6	65	theme	fungal	751:756	arg1	decay					758:762	the fungal decay	747:762	the fungal decay of tomatoes inoculated with Aspergillus niger and Penicillium citrinum	747:833	A combination of chitosan (CS) and EO was used to control the fungal decay of tomatoes inoculated with Aspergillus niger and Penicillium citrinum.
33937995	7	66	theme	Penicillium	942:952	arg1	spp					954:956	Aspergillus and Penicillium spp	926:956	spp	954:956	RESULTS Tomatoes obtained from local markets and supermarkets showed a high prevalence of Aspergillus and Penicillium spp.
33937995	9	67	theme	EO-	1388:1390	arg1	coatings					1405:1412	preservative potential, EO- and CS-based coatings	1364:1412	preservative potential, EO- and CS-based coatings	1364:1412	The combination of EO with chitosan (2%) as a coating, effectively controlled the fungal decay of tomatoes until the eighth day of storage at 25 °C. CONCLUSION Due to their edible nature, and their antifungal and preservative potential, EO- and CS-based coatings can be used to extend the shelf life of tomatoes and other agriculture commodities.
33937995	1	68	theme	spoilage	188:195	arg1	cause					174:178	a major cause	166:178	a major cause of food spoilage	166:195	BACKGROUND Fungal contamination is a major cause of food spoilage.
33937995	1	68	theme	spoilage	188:195	arg1	contamination					149:161	BACKGROUND Fungal contamination	131:161	BACKGROUND Fungal contamination	131:161	BACKGROUND Fungal contamination is a major cause of food spoilage.
33937995	3	69	from	prevalence	295:304	arg1	tomatoes					318:325	tomatoes	318:325	tomatoes	318:325	This study evaluates the prevalence of fungi in tomatoes and their control by using essential oil (EO) from sweet orange peel.
33937995	3	69	from	prevalence	295:304	arg1	control					337:343	their control	331:343	their control	331:343	This study evaluates the prevalence of fungi in tomatoes and their control by using essential oil (EO) from sweet orange peel.
33937995	6	70	theme	chitosan	706:713	arg1	combination					691:701	A combination	689:701	A combination of chitosan (CS) and EO	689:725	A combination of chitosan (CS) and EO was used to control the fungal decay of tomatoes inoculated with Aspergillus niger and Penicillium citrinum.
33937995	10	71	theme	synthetic	1564:1572	arg1	oil-					1508:1511	Essential oil-	1498:1511	Essential oil-	1498:1511	Essential oil- and CS-based coating can be used as alternative to synthetic preservatives, which are associated with various health hazards.
33937995	10	71	theme	synthetic	1564:1572	arg1	preservatives					1574:1586	alternative to synthetic preservatives	1549:1586	alternative to synthetic preservatives	1549:1586	Essential oil- and CS-based coating can be used as alternative to synthetic preservatives, which are associated with various health hazards.
33937995	10	71	theme	synthetic	1564:1572	arg1	coating					1526:1532	CS-based coating	1517:1532	CS-based coating	1517:1532	Essential oil- and CS-based coating can be used as alternative to synthetic preservatives, which are associated with various health hazards.
33937995	9	72	theme	storage	1282:1288	arg1	day					1275:1277	the eighth day	1264:1277	the eighth day of storage at 25 °C. CONCLUSION Due to their edible nature	1264:1336	The combination of EO with chitosan (2%) as a coating, effectively controlled the fungal decay of tomatoes until the eighth day of storage at 25 °C. CONCLUSION Due to their edible nature, and their antifungal and preservative potential, EO- and CS-based coatings can be used to extend the shelf life of tomatoes and other agriculture commodities.
33937995	8	73	theme	dried	1000:1004	arg1	peels					1006:1010	dried peels	1000:1010	dried peels	1000:1010	Essential oils extracted by ethanol from dried peels showed complete inhibition of A. niger and P. citrinum and hyphal degradation at a minimum inhibitory concentration (MIC) of 100 μL mL-1 .
33937995	2	74	theme	urgent	210:215	arg1	need					217:220	an urgent need	207:220	an urgent need to find and characterize natural preservatives	207:267	There is an urgent need to find and characterize natural preservatives.
33937995	7	75	theme	Aspergillus	926:936	arg1	spp					954:956	Aspergillus and Penicillium spp	926:956	spp	954:956	RESULTS Tomatoes obtained from local markets and supermarkets showed a high prevalence of Aspergillus and Penicillium spp.
33937995	9	76	theme	CS-based	1396:1403	arg1	coatings					1405:1412	preservative potential, EO- and CS-based coatings	1364:1412	preservative potential, EO- and CS-based coatings	1364:1412	The combination of EO with chitosan (2%) as a coating, effectively controlled the fungal decay of tomatoes until the eighth day of storage at 25 °C. CONCLUSION Due to their edible nature, and their antifungal and preservative potential, EO- and CS-based coatings can be used to extend the shelf life of tomatoes and other agriculture commodities.
33937995	5	77	dep	spectroscopy	548:559	arg1	analyses					617:624	analyses	617:624	analyses	617:624	Fourier transform infrared spectroscopy (FTIR) and gas chromatography-mass spectrometry (GC-MS) analyses were performed to identify the chemical composition of the EO.
33937995	8	78	theme	citrinum	1058:1065	arg1	inhibition					1028:1037	complete inhibition	1019:1037	complete inhibition of A. niger and P. citrinum	1019:1065	Essential oils extracted by ethanol from dried peels showed complete inhibition of A. niger and P. citrinum and hyphal degradation at a minimum inhibitory concentration (MIC) of 100 μL mL-1 .
33937995	8	78	theme	citrinum	1058:1065	arg1	degradation					1078:1088	hyphal degradation	1071:1088	hyphal degradation	1071:1088	Essential oils extracted by ethanol from dried peels showed complete inhibition of A. niger and P. citrinum and hyphal degradation at a minimum inhibitory concentration (MIC) of 100 μL mL-1 .
33937995	4	79	theme	fresh	442:446	arg1	peels					461:465	dried and fresh sweet orange peels	432:465	dried and fresh sweet orange peels	432:465	Essential oils were extracted from dried and fresh sweet orange peels by using n-hexane and ethanol as extraction solvents.
33937995	9	80	theme	25 °C.	1293:1298	arg1	CONCLUSION					1300:1309	25 °C. CONCLUSION	1293:1309	25 °C. CONCLUSION	1293:1309	The combination of EO with chitosan (2%) as a coating, effectively controlled the fungal decay of tomatoes until the eighth day of storage at 25 °C. CONCLUSION Due to their edible nature, and their antifungal and preservative potential, EO- and CS-based coatings can be used to extend the shelf life of tomatoes and other agriculture commodities.
33937995	7	81	theme	high	907:910	arg1	prevalence					912:921	a high prevalence	905:921	a high prevalence of Aspergillus and Penicillium spp	905:956	RESULTS Tomatoes obtained from local markets and supermarkets showed a high prevalence of Aspergillus and Penicillium spp.
33937995	5	82	dep	Fourier	521:527	arg1	transform					529:537	transform	529:537	transform infrared spectroscopy (FTIR) and gas chromatography-mass spectrometry (GC-MS) analyses	529:624	Fourier transform infrared spectroscopy (FTIR) and gas chromatography-mass spectrometry (GC-MS) analyses were performed to identify the chemical composition of the EO.
33937995	5	83	theme	EO	685:686	arg1	composition					666:676	the chemical composition	653:676	the chemical composition of the EO	653:686	Fourier transform infrared spectroscopy (FTIR) and gas chromatography-mass spectrometry (GC-MS) analyses were performed to identify the chemical composition of the EO.
32502191	4	0	theme	yearling	758:765	arg1	bulls					767:771	Twenty-four Charolais yearling bulls	736:771	Twenty-four Charolais yearling bulls	736:771	Twenty-four Charolais yearling bulls were randomly divided into two groups and fed during 8 months, from weaning to slaughter, either 1) a high starch diet based on corn silage supplying a balanced N to energy ratio at the rumen level (starch) or 2) a high fiber diet based on grass silage supplying an excess of rumen degradable N (fiber).
32502191	8	1	theme	N	1569:1569	arg1	discrimination					1580:1593	the N isotopic discrimination	1565:1593	the N isotopic discrimination occurring before absorption	1565:1621	For both diets, the digestive contribution (i.e. the N isotopic discrimination occurring before absorption) to the Δ15N observed in animal tissues accounted for 65 ± 11%, leaving only one third to the contribution of post-absorptive metabolism.
32502191	5	2	theme	animal	1194:1199	arg1	tissues					1201:1207	animal tissues	1194:1207	animal tissues (duodenum, liver and muscle)	1194:1236	All animals were slaughtered and samples of different digestive pools (ruminal, duodenal, ileal and fecal contents), animal tissues (duodenum, liver and muscle), blood and urine were collected for each animal.
32502191	5	2	theme	animal	1194:1199	arg1	pools					1141:1145	different digestive pools	1121:1145	different digestive pools (ruminal, duodenal, ileal and fecal contents)	1121:1191	All animals were slaughtered and samples of different digestive pools (ruminal, duodenal, ileal and fecal contents), animal tissues (duodenum, liver and muscle), blood and urine were collected for each animal.
32502191	12	3	theme	digestive	2277:2285	arg1	phenomena					2317:2325	both digestive and post-absorptive metabolic phenomena	2272:2325	both digestive and post-absorptive metabolic phenomena	2272:2325	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	12	4	theme	post-absorptive	2291:2305	arg1	phenomena					2317:2325	both digestive and post-absorptive metabolic phenomena	2272:2325	both digestive and post-absorptive metabolic phenomena	2272:2325	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	15	5	theme	N	2824:2824	arg1	use					2817:2819	the contrasted use	2802:2819	the contrasted use of N	2802:2824	Our results highlight the contrasted use of N at the rumen level between the two experimental diets and suggests the need for different equations to predict FCE or NUE from Δ15N according to the type of diet.
32502191	14	6	theme	diet	2744:2747	arg1	interaction					2756:2766	diet x pool interaction	2744:2766	diet x pool interaction	2744:2766	The within-diet relationship (n = 12) between Δ15N and feed efficiency was not as strong and consistent as the overall relationship, with contrasted responses between the two diets for specific pools (diet x pool interaction; P ≤ 0.01).
32502191	14	6	theme	diet	2744:2747	arg1	pools					2737:2741	specific pools	2728:2741	specific pools (diet x pool interaction; P ≤ 0.01)	2728:2777	The within-diet relationship (n = 12) between Δ15N and feed efficiency was not as strong and consistent as the overall relationship, with contrasted responses between the two diets for specific pools (diet x pool interaction; P ≤ 0.01).
32502191	11	7	from	role	1971:1974	arg1	discrimination					2003:2016	N isotopic discrimination	1992:2016	N isotopic discrimination	1992:2016	Δ15N = 2.24 ± 0.41‰), highlighting the key role of the rumen on N isotopic discrimination.
32502191	6	8	theme	Ruminal	1287:1293	arg1	content					1295:1301	Ruminal content	1287:1301	Ruminal content	1287:1301	Ruminal content was further used to isolate liquid-associated bacteria (LAB), protozoa and free ammonia, while plasma proteins were obtained from blood.
32502191	4	9	theme	rumen	959:963	arg1	level					965:969	the rumen level	955:969	the rumen level (starch)	955:978	Twenty-four Charolais yearling bulls were randomly divided into two groups and fed during 8 months, from weaning to slaughter, either 1) a high starch diet based on corn silage supplying a balanced N to energy ratio at the rumen level (starch) or 2) a high fiber diet based on grass silage supplying an excess of rumen degradable N (fiber).
32502191	4	9	theme	rumen	959:963	arg1	starch					972:977	starch	972:977	starch	972:977	Twenty-four Charolais yearling bulls were randomly divided into two groups and fed during 8 months, from weaning to slaughter, either 1) a high starch diet based on corn silage supplying a balanced N to energy ratio at the rumen level (starch) or 2) a high fiber diet based on grass silage supplying an excess of rumen degradable N (fiber).
32502191	4	10	dep	diet	887:890	arg1	1					870:870	1	870:870	1	870:870	Twenty-four Charolais yearling bulls were randomly divided into two groups and fed during 8 months, from weaning to slaughter, either 1) a high starch diet based on corn silage supplying a balanced N to energy ratio at the rumen level (starch) or 2) a high fiber diet based on grass silage supplying an excess of rumen degradable N (fiber).
32502191	5	11	theme	fecal	1177:1181	arg1	pools					1141:1145	different digestive pools	1121:1145	different digestive pools (ruminal, duodenal, ileal and fecal contents)	1121:1191	All animals were slaughtered and samples of different digestive pools (ruminal, duodenal, ileal and fecal contents), animal tissues (duodenum, liver and muscle), blood and urine were collected for each animal.
32502191	5	11	theme	fecal	1177:1181	arg1	contents					1183:1190	ruminal, duodenal, ileal and fecal contents	1148:1190	ruminal, duodenal, ileal and fecal contents	1148:1190	All animals were slaughtered and samples of different digestive pools (ruminal, duodenal, ileal and fecal contents), animal tissues (duodenum, liver and muscle), blood and urine were collected for each animal.
32502191	16	12	theme	fattening	3214:3222	arg1	bulls					3233:3237	fattening yearling bulls	3214:3237	fattening yearling bulls	3214:3237	In conclusion, rumen digestion and associated microbial activity can play an important role on N isotopic discrimination so rumen effect related to diet may interfere with the relationship between Δ15N and feed efficiency in fattening yearling bulls.
32502191	13	13	theme	≤	2414:2414	arg1	P					2412:2412	P ≤ 0.05	2412:2419	P ≤ 0.05	2412:2419	Fiber diet compared to starch diet had a lower feed efficiency and promoted higher (P ≤ 0.05) Δ15N values across all post-absorptive metabolic pools and some digestive pools (ruminal, duodenal, and ileal contents).
32502191	7	14	theme	isotopic	1494:1501	arg1	composition					1503:1513	their N isotopic composition	1486:1513	their N isotopic composition	1486:1513	All samples along with feed were analyzed for their N isotopic composition.
32502191	4	15	dep	diet	999:1002	arg1	2					983:983	2	983:983	2	983:983	Twenty-four Charolais yearling bulls were randomly divided into two groups and fed during 8 months, from weaning to slaughter, either 1) a high starch diet based on corn silage supplying a balanced N to energy ratio at the rumen level (starch) or 2) a high fiber diet based on grass silage supplying an excess of rumen degradable N (fiber).
32502191	4	16	theme	Charolais	748:756	arg1	bulls					767:771	Twenty-four Charolais yearling bulls	736:771	Twenty-four Charolais yearling bulls	736:771	Twenty-four Charolais yearling bulls were randomly divided into two groups and fed during 8 months, from weaning to slaughter, either 1) a high starch diet based on corn silage supplying a balanced N to energy ratio at the rumen level (starch) or 2) a high fiber diet based on grass silage supplying an excess of rumen degradable N (fiber).
32502191	15	17	theme	diet	2983:2986	arg1	type					2975:2978	the type	2971:2978	the type of diet	2971:2986	Our results highlight the contrasted use of N at the rumen level between the two experimental diets and suggests the need for different equations to predict FCE or NUE from Δ15N according to the type of diet.
32502191	4	18	theme	energy	939:944	arg1	ratio					946:950	energy ratio	939:950	energy ratio at the rumen level (starch)	939:978	Twenty-four Charolais yearling bulls were randomly divided into two groups and fed during 8 months, from weaning to slaughter, either 1) a high starch diet based on corn silage supplying a balanced N to energy ratio at the rumen level (starch) or 2) a high fiber diet based on grass silage supplying an excess of rumen degradable N (fiber).
32502191	1	19	from	diet	248:251	arg1	abundance					202:210	natural 15N abundance	190:210	natural 15N abundance	190:210	Nitrogen (N) isotopic discrimination (i.e. the difference in natural 15N abundance between the animal proteins and the diet; Δ15N) is known to correlate with N use efficiency (NUE) and feed conversion efficiency (FCE) in ruminants.
32502191	1	20	theme	conversion	319:328	arg1	FCE					342:344	FCE	342:344	FCE	342:344	Nitrogen (N) isotopic discrimination (i.e. the difference in natural 15N abundance between the animal proteins and the diet; Δ15N) is known to correlate with N use efficiency (NUE) and feed conversion efficiency (FCE) in ruminants.
32502191	1	20	theme	conversion	319:328	arg1	efficiency					330:339	feed conversion efficiency	314:339	feed conversion efficiency (FCE) in ruminants	314:358	Nitrogen (N) isotopic discrimination (i.e. the difference in natural 15N abundance between the animal proteins and the diet; Δ15N) is known to correlate with N use efficiency (NUE) and feed conversion efficiency (FCE) in ruminants.
32502191	2	21	theme	discrimination	463:476	arg1	pathways					478:485	isotopic discrimination pathways	454:485	isotopic discrimination pathways that may differ with the nature of diets	454:526	However, results from the literature are not always consistent across studies, likely due to isotopic discrimination pathways that may differ with the nature of diets.
32502191	13	22	theme	feed	2375:2378	arg1	efficiency					2380:2389	a lower feed efficiency	2367:2389	a lower feed efficiency	2367:2389	Fiber diet compared to starch diet had a lower feed efficiency and promoted higher (P ≤ 0.05) Δ15N values across all post-absorptive metabolic pools and some digestive pools (ruminal, duodenal, and ileal contents).
32502191	1	23	theme	15N	198:200	arg1	abundance					202:210	natural 15N abundance	190:210	natural 15N abundance	190:210	Nitrogen (N) isotopic discrimination (i.e. the difference in natural 15N abundance between the animal proteins and the diet; Δ15N) is known to correlate with N use efficiency (NUE) and feed conversion efficiency (FCE) in ruminants.
32502191	9	24	dep	rumen	1847:1851	arg1	av					1854:1855	av	1854:1855	av	1854:1855	Concerning the Δ15N in digestive pools, the majority of these changes occurred in the rumen (av.
32502191	13	25	dep	higher	2404:2409	arg1	P					2412:2412	P ≤ 0.05	2412:2419	P ≤ 0.05	2412:2419	Fiber diet compared to starch diet had a lower feed efficiency and promoted higher (P ≤ 0.05) Δ15N values across all post-absorptive metabolic pools and some digestive pools (ruminal, duodenal, and ileal contents).
32502191	8	26	theme	animal	1648:1653	arg1	tissues					1655:1661	animal tissues	1648:1661	animal tissues	1648:1661	For both diets, the digestive contribution (i.e. the N isotopic discrimination occurring before absorption) to the Δ15N observed in animal tissues accounted for 65 ± 11%, leaving only one third to the contribution of post-absorptive metabolism.
32502191	1	27	from	difference	176:185	arg1	abundance					202:210	natural 15N abundance	190:210	natural 15N abundance	190:210	Nitrogen (N) isotopic discrimination (i.e. the difference in natural 15N abundance between the animal proteins and the diet; Δ15N) is known to correlate with N use efficiency (NUE) and feed conversion efficiency (FCE) in ruminants.
32502191	10	28	theme	minor	1890:1894	arg1	enrichments					1900:1910	only minor 15N enrichments	1885:1910	only minor 15N enrichments	1885:1910	Δ15N = 2.12 ± 0.66‰), with only minor 15N enrichments thereafter (av.
32502191	12	29	theme	≤	2231:2231	arg1	P					2229:2229	P ≤ 0.01	2229:2236	P ≤ 0.01	2229:2236	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	6	30	theme	plasma	1398:1403	arg1	proteins					1405:1412	plasma proteins	1398:1412	plasma proteins	1398:1412	Ruminal content was further used to isolate liquid-associated bacteria (LAB), protozoa and free ammonia, while plasma proteins were obtained from blood.
32502191	4	31	theme	fiber	993:997	arg1	diet					999:1002	a high fiber diet	986:1002	2) a high fiber diet based on grass silage supplying an excess of rumen degradable N (fiber)	983:1074	Twenty-four Charolais yearling bulls were randomly divided into two groups and fed during 8 months, from weaning to slaughter, either 1) a high starch diet based on corn silage supplying a balanced N to energy ratio at the rumen level (starch) or 2) a high fiber diet based on grass silage supplying an excess of rumen degradable N (fiber).
32502191	12	32	theme	r	2219:2219	arg1	<					2221:2221	0.52 < r < 0.73	2212:2226	duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73	2156:2226	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	14	33	theme	specific	2728:2735	arg1	interaction					2756:2766	diet x pool interaction	2744:2766	diet x pool interaction	2744:2766	The within-diet relationship (n = 12) between Δ15N and feed efficiency was not as strong and consistent as the overall relationship, with contrasted responses between the two diets for specific pools (diet x pool interaction; P ≤ 0.01).
32502191	14	33	theme	specific	2728:2735	arg1	pools					2737:2741	specific pools	2728:2741	specific pools (diet x pool interaction; P ≤ 0.01)	2728:2777	The within-diet relationship (n = 12) between Δ15N and feed efficiency was not as strong and consistent as the overall relationship, with contrasted responses between the two diets for specific pools (diet x pool interaction; P ≤ 0.01).
32502191	1	34	theme	Nitrogen	129:136	arg1	discrimination					151:164	Nitrogen (N) isotopic discrimination	129:164	Nitrogen (N) isotopic discrimination (i.e. the difference in natural 15N abundance between the animal proteins and the diet; Δ15N)	129:258	Nitrogen (N) isotopic discrimination (i.e. the difference in natural 15N abundance between the animal proteins and the diet; Δ15N) is known to correlate with N use efficiency (NUE) and feed conversion efficiency (FCE) in ruminants.
32502191	1	34	theme	Nitrogen	129:136	arg1	difference					176:185	the difference	172:185	the difference in natural 15N abundance between the animal proteins	172:238	Nitrogen (N) isotopic discrimination (i.e. the difference in natural 15N abundance between the animal proteins and the diet; Δ15N) is known to correlate with N use efficiency (NUE) and feed conversion efficiency (FCE) in ruminants.
32502191	1	34	theme	Nitrogen	129:136	arg1	diet					248:251	the diet	244:251	the diet	244:251	Nitrogen (N) isotopic discrimination (i.e. the difference in natural 15N abundance between the animal proteins and the diet; Δ15N) is known to correlate with N use efficiency (NUE) and feed conversion efficiency (FCE) in ruminants.
32502191	3	35	theme	contrasted	718:727	arg1	diets					729:733	two contrasted diets	714:733	two contrasted diets	714:733	The objective of the present study was to assess at which level, from rumen to tissues, Δ15N originates and becomes related to NUE and FCE in fattening yearling bulls when they are fed two contrasted diets.
32502191	15	36	theme	experimental	2861:2872	arg1	diets					2874:2878	the two experimental diets	2853:2878	the two experimental diets	2853:2878	Our results highlight the contrasted use of N at the rumen level between the two experimental diets and suggests the need for different equations to predict FCE or NUE from Δ15N according to the type of diet.
32502191	3	37	theme	fattening	671:679	arg1	bulls					690:694	fattening yearling bulls	671:694	fattening yearling bulls	671:694	The objective of the present study was to assess at which level, from rumen to tissues, Δ15N originates and becomes related to NUE and FCE in fattening yearling bulls when they are fed two contrasted diets.
32502191	0	38	theme	relationship	48:59	arg1	origin					4:9	The origin	0:9	The origin of N isotopic discrimination and its relationship with feed efficiency in fattening yearling bulls	0:108	The origin of N isotopic discrimination and its relationship with feed efficiency in fattening yearling bulls is diet-dependent.
32502191	0	38	theme	relationship	48:59	arg1	diet-dependent					113:126	diet-dependent	113:126	diet-dependent	113:126	The origin of N isotopic discrimination and its relationship with feed efficiency in fattening yearling bulls is diet-dependent.
32502191	1	39	theme	N	287:287	arg1	efficiency					293:302	N use efficiency	287:302	N use efficiency (NUE)	287:308	Nitrogen (N) isotopic discrimination (i.e. the difference in natural 15N abundance between the animal proteins and the diet; Δ15N) is known to correlate with N use efficiency (NUE) and feed conversion efficiency (FCE) in ruminants.
32502191	1	39	theme	N	287:287	arg1	NUE					305:307	NUE	305:307	NUE	305:307	Nitrogen (N) isotopic discrimination (i.e. the difference in natural 15N abundance between the animal proteins and the diet; Δ15N) is known to correlate with N use efficiency (NUE) and feed conversion efficiency (FCE) in ruminants.
32502191	1	40	theme	N	139:139	arg1	discrimination					151:164	Nitrogen (N) isotopic discrimination	129:164	Nitrogen (N) isotopic discrimination (i.e. the difference in natural 15N abundance between the animal proteins and the diet; Δ15N)	129:258	Nitrogen (N) isotopic discrimination (i.e. the difference in natural 15N abundance between the animal proteins and the diet; Δ15N) is known to correlate with N use efficiency (NUE) and feed conversion efficiency (FCE) in ruminants.
32502191	1	40	theme	N	139:139	arg1	difference					176:185	the difference	172:185	the difference in natural 15N abundance between the animal proteins	172:238	Nitrogen (N) isotopic discrimination (i.e. the difference in natural 15N abundance between the animal proteins and the diet; Δ15N) is known to correlate with N use efficiency (NUE) and feed conversion efficiency (FCE) in ruminants.
32502191	1	40	theme	N	139:139	arg1	diet					248:251	the diet	244:251	the diet	244:251	Nitrogen (N) isotopic discrimination (i.e. the difference in natural 15N abundance between the animal proteins and the diet; Δ15N) is known to correlate with N use efficiency (NUE) and feed conversion efficiency (FCE) in ruminants.
32502191	15	41	theme	rumen	2833:2837	arg1	level					2839:2843	the rumen level	2829:2843	the rumen level between the two experimental diets	2829:2878	Our results highlight the contrasted use of N at the rumen level between the two experimental diets and suggests the need for different equations to predict FCE or NUE from Δ15N according to the type of diet.
32502191	13	42	theme	ileal	2526:2530	arg1	contents					2532:2539	ileal contents	2526:2539	ileal contents	2526:2539	Fiber diet compared to starch diet had a lower feed efficiency and promoted higher (P ≤ 0.05) Δ15N values across all post-absorptive metabolic pools and some digestive pools (ruminal, duodenal, and ileal contents).
32502191	3	43	from	NUE	656:658	arg1	bulls					690:694	fattening yearling bulls	671:694	fattening yearling bulls	671:694	The objective of the present study was to assess at which level, from rumen to tissues, Δ15N originates and becomes related to NUE and FCE in fattening yearling bulls when they are fed two contrasted diets.
32502191	0	44	theme	feed	66:69	arg1	efficiency					71:80	feed efficiency	66:80	feed efficiency	66:80	The origin of N isotopic discrimination and its relationship with feed efficiency in fattening yearling bulls is diet-dependent.
32502191	1	45	theme	isotopic	142:149	arg1	discrimination					151:164	Nitrogen (N) isotopic discrimination	129:164	Nitrogen (N) isotopic discrimination (i.e. the difference in natural 15N abundance between the animal proteins and the diet; Δ15N)	129:258	Nitrogen (N) isotopic discrimination (i.e. the difference in natural 15N abundance between the animal proteins and the diet; Δ15N) is known to correlate with N use efficiency (NUE) and feed conversion efficiency (FCE) in ruminants.
32502191	1	45	theme	isotopic	142:149	arg1	difference					176:185	the difference	172:185	the difference in natural 15N abundance between the animal proteins	172:238	Nitrogen (N) isotopic discrimination (i.e. the difference in natural 15N abundance between the animal proteins and the diet; Δ15N) is known to correlate with N use efficiency (NUE) and feed conversion efficiency (FCE) in ruminants.
32502191	1	45	theme	isotopic	142:149	arg1	diet					248:251	the diet	244:251	the diet	244:251	Nitrogen (N) isotopic discrimination (i.e. the difference in natural 15N abundance between the animal proteins and the diet; Δ15N) is known to correlate with N use efficiency (NUE) and feed conversion efficiency (FCE) in ruminants.
32502191	14	46	theme	≤	2771:2771	arg1	P					2769:2769	P ≤ 0.01	2769:2776	P ≤ 0.01	2769:2776	The within-diet relationship (n = 12) between Δ15N and feed efficiency was not as strong and consistent as the overall relationship, with contrasted responses between the two diets for specific pools (diet x pool interaction; P ≤ 0.01).
32502191	3	47	from	FCE	664:666	arg1	bulls					690:694	fattening yearling bulls	671:694	fattening yearling bulls	671:694	The objective of the present study was to assess at which level, from rumen to tissues, Δ15N originates and becomes related to NUE and FCE in fattening yearling bulls when they are fed two contrasted diets.
32502191	11	48	theme	isotopic	1994:2001	arg1	discrimination					2003:2016	N isotopic discrimination	1992:2016	N isotopic discrimination	1992:2016	Δ15N = 2.24 ± 0.41‰), highlighting the key role of the rumen on N isotopic discrimination.
32502191	13	49	contain	had	2363:2365	arg2	efficiency					2380:2389	a lower feed efficiency	2367:2389	a lower feed efficiency	2367:2389	Fiber diet compared to starch diet had a lower feed efficiency and promoted higher (P ≤ 0.05) Δ15N values across all post-absorptive metabolic pools and some digestive pools (ruminal, duodenal, and ileal contents).
32502191	13	49	contain	had	2363:2365	arg1	diet					2334:2337	Fiber diet	2328:2337	Fiber diet compared to starch diet	2328:2361	Fiber diet compared to starch diet had a lower feed efficiency and promoted higher (P ≤ 0.05) Δ15N values across all post-absorptive metabolic pools and some digestive pools (ruminal, duodenal, and ileal contents).
32502191	8	50	theme	digestive	1536:1544	arg1	contribution					1546:1557	the digestive contribution	1532:1557	the digestive contribution (i.e. the N isotopic discrimination occurring before absorption) to the Δ15N observed in animal tissues	1532:1661	For both diets, the digestive contribution (i.e. the N isotopic discrimination occurring before absorption) to the Δ15N observed in animal tissues accounted for 65 ± 11%, leaving only one third to the contribution of post-absorptive metabolism.
32502191	15	51	theme	different	2906:2914	arg1	equations					2916:2924	different equations	2906:2924	different equations	2906:2924	Our results highlight the contrasted use of N at the rumen level between the two experimental diets and suggests the need for different equations to predict FCE or NUE from Δ15N according to the type of diet.
32502191	0	52	theme	yearling	95:102	arg1	bulls					104:108	fattening yearling bulls	85:108	fattening yearling bulls	85:108	The origin of N isotopic discrimination and its relationship with feed efficiency in fattening yearling bulls is diet-dependent.
32502191	1	53	from	efficiency	293:302	arg1	ruminants					350:358	ruminants	350:358	ruminants	350:358	Nitrogen (N) isotopic discrimination (i.e. the difference in natural 15N abundance between the animal proteins and the diet; Δ15N) is known to correlate with N use efficiency (NUE) and feed conversion efficiency (FCE) in ruminants.
32502191	4	54	theme	starch	880:885	arg1	diet					887:890	a high starch diet	873:890	a high starch diet based on corn silage supplying a balanced N to energy ratio at the rumen level (starch)	873:978	Twenty-four Charolais yearling bulls were randomly divided into two groups and fed during 8 months, from weaning to slaughter, either 1) a high starch diet based on corn silage supplying a balanced N to energy ratio at the rumen level (starch) or 2) a high fiber diet based on grass silage supplying an excess of rumen degradable N (fiber).
32502191	14	55	theme	pool	2751:2754	arg1	interaction					2756:2766	diet x pool interaction	2744:2766	diet x pool interaction	2744:2766	The within-diet relationship (n = 12) between Δ15N and feed efficiency was not as strong and consistent as the overall relationship, with contrasted responses between the two diets for specific pools (diet x pool interaction; P ≤ 0.01).
32502191	14	55	theme	pool	2751:2754	arg1	pools					2737:2741	specific pools	2728:2741	specific pools (diet x pool interaction; P ≤ 0.01)	2728:2777	The within-diet relationship (n = 12) between Δ15N and feed efficiency was not as strong and consistent as the overall relationship, with contrasted responses between the two diets for specific pools (diet x pool interaction; P ≤ 0.01).
32502191	16	56	theme	N	3084:3084	arg1	discrimination					3095:3108	N isotopic discrimination	3084:3108	N isotopic discrimination so rumen effect related to diet may interfere with the relationship between Δ15N and feed efficiency in fattening yearling bulls	3084:3237	In conclusion, rumen digestion and associated microbial activity can play an important role on N isotopic discrimination so rumen effect related to diet may interfere with the relationship between Δ15N and feed efficiency in fattening yearling bulls.
32502191	5	57	theme	digestive	1131:1139	arg1	tissues					1201:1207	animal tissues	1194:1207	animal tissues (duodenum, liver and muscle)	1194:1236	All animals were slaughtered and samples of different digestive pools (ruminal, duodenal, ileal and fecal contents), animal tissues (duodenum, liver and muscle), blood and urine were collected for each animal.
32502191	5	57	theme	digestive	1131:1139	arg1	pools					1141:1145	different digestive pools	1121:1145	different digestive pools (ruminal, duodenal, ileal and fecal contents)	1121:1191	All animals were slaughtered and samples of different digestive pools (ruminal, duodenal, ileal and fecal contents), animal tissues (duodenum, liver and muscle), blood and urine were collected for each animal.
32502191	5	57	theme	digestive	1131:1139	arg1	blood					1239:1243	blood	1239:1243	blood	1239:1243	All animals were slaughtered and samples of different digestive pools (ruminal, duodenal, ileal and fecal contents), animal tissues (duodenum, liver and muscle), blood and urine were collected for each animal.
32502191	5	57	theme	digestive	1131:1139	arg1	urine					1249:1253	urine	1249:1253	urine	1249:1253	All animals were slaughtered and samples of different digestive pools (ruminal, duodenal, ileal and fecal contents), animal tissues (duodenum, liver and muscle), blood and urine were collected for each animal.
32502191	5	57	theme	digestive	1131:1139	arg1	contents					1183:1190	ruminal, duodenal, ileal and fecal contents	1148:1190	ruminal, duodenal, ileal and fecal contents	1148:1190	All animals were slaughtered and samples of different digestive pools (ruminal, duodenal, ileal and fecal contents), animal tissues (duodenum, liver and muscle), blood and urine were collected for each animal.
32502191	16	58	theme	feed	3195:3198	arg1	efficiency					3200:3209	feed efficiency	3195:3209	feed efficiency	3195:3209	In conclusion, rumen digestion and associated microbial activity can play an important role on N isotopic discrimination so rumen effect related to diet may interfere with the relationship between Δ15N and feed efficiency in fattening yearling bulls.
32502191	14	59	theme	within-diet	2547:2557	arg1	relationship					2559:2570	The within-diet relationship	2543:2570	The within-diet relationship (n = 12) between Δ15N	2543:2592	The within-diet relationship (n = 12) between Δ15N and feed efficiency was not as strong and consistent as the overall relationship, with contrasted responses between the two diets for specific pools (diet x pool interaction; P ≤ 0.01).
32502191	14	59	theme	within-diet	2547:2557	arg1	strong					2625:2630	strong	2625:2630	strong	2625:2630	The within-diet relationship (n = 12) between Δ15N and feed efficiency was not as strong and consistent as the overall relationship, with contrasted responses between the two diets for specific pools (diet x pool interaction; P ≤ 0.01).
32502191	14	59	theme	within-diet	2547:2557	arg1	n					2573:2573	n = 12	2573:2578	n = 12	2573:2578	The within-diet relationship (n = 12) between Δ15N and feed efficiency was not as strong and consistent as the overall relationship, with contrasted responses between the two diets for specific pools (diet x pool interaction; P ≤ 0.01).
32502191	4	60	theme	grass	1013:1017	arg1	silage					1019:1024	grass silage	1013:1024	grass silage supplying an excess of rumen degradable N (fiber)	1013:1074	Twenty-four Charolais yearling bulls were randomly divided into two groups and fed during 8 months, from weaning to slaughter, either 1) a high starch diet based on corn silage supplying a balanced N to energy ratio at the rumen level (starch) or 2) a high fiber diet based on grass silage supplying an excess of rumen degradable N (fiber).
32502191	12	61	theme	=	2065:2065	arg1	n					2063:2063	n = 24	2063:2068	n = 24	2063:2068	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	12	61	theme	=	2065:2065	arg1	relationship					2049:2060	A strong, significant overall relationship	2019:2060	relationship	2049:2060	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	16	62	theme	rumen	3004:3008	arg1	digestion					3010:3018	rumen digestion	3004:3018	rumen digestion	3004:3018	In conclusion, rumen digestion and associated microbial activity can play an important role on N isotopic discrimination so rumen effect related to diet may interfere with the relationship between Δ15N and feed efficiency in fattening yearling bulls.
32502191	0	63	theme	isotopic	16:23	arg1	discrimination					25:38	N isotopic discrimination	14:38	N isotopic discrimination	14:38	The origin of N isotopic discrimination and its relationship with feed efficiency in fattening yearling bulls is diet-dependent.
32502191	3	64	dep	tissues	608:614	arg1	rumen					599:603	rumen	599:603	rumen	599:603	The objective of the present study was to assess at which level, from rumen to tissues, Δ15N originates and becomes related to NUE and FCE in fattening yearling bulls when they are fed two contrasted diets.
32502191	16	65	from	efficiency	3200:3209	arg1	bulls					3233:3237	fattening yearling bulls	3214:3237	fattening yearling bulls	3214:3237	In conclusion, rumen digestion and associated microbial activity can play an important role on N isotopic discrimination so rumen effect related to diet may interfere with the relationship between Δ15N and feed efficiency in fattening yearling bulls.
32502191	16	66	from	Δ15N	3186:3189	arg1	bulls					3233:3237	fattening yearling bulls	3214:3237	fattening yearling bulls	3214:3237	In conclusion, rumen digestion and associated microbial activity can play an important role on N isotopic discrimination so rumen effect related to diet may interfere with the relationship between Δ15N and feed efficiency in fattening yearling bulls.
32502191	3	67	theme	study	558:562	arg1	objective					533:541	The objective	529:541	The objective of the present study	529:562	The objective of the present study was to assess at which level, from rumen to tissues, Δ15N originates and becomes related to NUE and FCE in fattening yearling bulls when they are fed two contrasted diets.
32502191	8	68	theme	metabolism	1749:1758	arg1	contribution					1717:1728	the contribution	1713:1728	the contribution of post-absorptive metabolism	1713:1758	For both diets, the digestive contribution (i.e. the N isotopic discrimination occurring before absorption) to the Δ15N observed in animal tissues accounted for 65 ± 11%, leaving only one third to the contribution of post-absorptive metabolism.
32502191	4	69	theme	balanced	925:932	arg1	N					934:934	a balanced N	923:934	a balanced N	923:934	Twenty-four Charolais yearling bulls were randomly divided into two groups and fed during 8 months, from weaning to slaughter, either 1) a high starch diet based on corn silage supplying a balanced N to energy ratio at the rumen level (starch) or 2) a high fiber diet based on grass silage supplying an excess of rumen degradable N (fiber).
32502191	12	70	theme	post-absorptive	2124:2138	arg1	proteins					2202:2209	plasma proteins	2195:2209	plasma proteins	2195:2209	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	12	70	theme	post-absorptive	2124:2138	arg1	tissues					2183:2189	muscle tissues	2176:2189	muscle tissues	2176:2189	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	12	70	theme	post-absorptive	2124:2138	arg1	duodenum					2156:2163	duodenum	2156:2163	duodenum	2156:2163	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	12	70	theme	post-absorptive	2124:2138	arg1	pool					2150:2153	any post-absorptive metabolic pool	2120:2153	any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01)	2120:2237	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	12	70	theme	post-absorptive	2124:2138	arg1	liver					2166:2170	liver	2166:2170	liver	2166:2170	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	4	71	theme	rumen	1049:1053	arg1	N					1066:1066	rumen degradable N	1049:1066	rumen degradable N (fiber)	1049:1074	Twenty-four Charolais yearling bulls were randomly divided into two groups and fed during 8 months, from weaning to slaughter, either 1) a high starch diet based on corn silage supplying a balanced N to energy ratio at the rumen level (starch) or 2) a high fiber diet based on grass silage supplying an excess of rumen degradable N (fiber).
32502191	4	71	theme	rumen	1049:1053	arg1	fiber					1069:1073	fiber	1069:1073	fiber	1069:1073	Twenty-four Charolais yearling bulls were randomly divided into two groups and fed during 8 months, from weaning to slaughter, either 1) a high starch diet based on corn silage supplying a balanced N to energy ratio at the rumen level (starch) or 2) a high fiber diet based on grass silage supplying an excess of rumen degradable N (fiber).
32502191	16	72	theme	microbial	3035:3043	arg1	activity					3045:3052	associated microbial activity	3024:3052	associated microbial activity	3024:3052	In conclusion, rumen digestion and associated microbial activity can play an important role on N isotopic discrimination so rumen effect related to diet may interfere with the relationship between Δ15N and feed efficiency in fattening yearling bulls.
32502191	13	73	theme	post-absorptive	2445:2459	arg1	pools					2471:2475	all post-absorptive metabolic pools	2441:2475	all post-absorptive metabolic pools	2441:2475	Fiber diet compared to starch diet had a lower feed efficiency and promoted higher (P ≤ 0.05) Δ15N values across all post-absorptive metabolic pools and some digestive pools (ruminal, duodenal, and ileal contents).
32502191	6	74	used	used	1315:1318	arg2	content					1295:1301	Ruminal content	1287:1301	Ruminal content	1287:1301	Ruminal content was further used to isolate liquid-associated bacteria (LAB), protozoa and free ammonia, while plasma proteins were obtained from blood.
32502191	4	75	theme	N	1066:1066	arg1	excess					1039:1044	an excess	1036:1044	an excess of rumen degradable N (fiber)	1036:1074	Twenty-four Charolais yearling bulls were randomly divided into two groups and fed during 8 months, from weaning to slaughter, either 1) a high starch diet based on corn silage supplying a balanced N to energy ratio at the rumen level (starch) or 2) a high fiber diet based on grass silage supplying an excess of rumen degradable N (fiber).
32502191	1	76	dep	difference	176:185	arg1	i.e.					167:170	i.e.	167:170	i.e.	167:170	Nitrogen (N) isotopic discrimination (i.e. the difference in natural 15N abundance between the animal proteins and the diet; Δ15N) is known to correlate with N use efficiency (NUE) and feed conversion efficiency (FCE) in ruminants.
32502191	1	76	dep	difference	176:185	arg1	Δ15N					254:257	Δ15N	254:257	Δ15N	254:257	Nitrogen (N) isotopic discrimination (i.e. the difference in natural 15N abundance between the animal proteins and the diet; Δ15N) is known to correlate with N use efficiency (NUE) and feed conversion efficiency (FCE) in ruminants.
32502191	12	77	dep	duodenum	2156:2163	arg1	P					2229:2229	P ≤ 0.01	2229:2236	P ≤ 0.01	2229:2236	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	12	77	dep	duodenum	2156:2163	arg1	<					2221:2221	0.52 < r < 0.73	2212:2226	duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73	2156:2226	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	14	78	dep	interaction	2756:2766	arg1	P					2769:2769	P ≤ 0.01	2769:2776	P ≤ 0.01	2769:2776	The within-diet relationship (n = 12) between Δ15N and feed efficiency was not as strong and consistent as the overall relationship, with contrasted responses between the two diets for specific pools (diet x pool interaction; P ≤ 0.01).
32502191	4	79	theme	corn	901:904	arg1	silage					906:911	corn silage	901:911	corn silage supplying a balanced N to energy ratio at the rumen level (starch)	901:978	Twenty-four Charolais yearling bulls were randomly divided into two groups and fed during 8 months, from weaning to slaughter, either 1) a high starch diet based on corn silage supplying a balanced N to energy ratio at the rumen level (starch) or 2) a high fiber diet based on grass silage supplying an excess of rumen degradable N (fiber).
32502191	8	80	theme	isotopic	1571:1578	arg1	discrimination					1580:1593	the N isotopic discrimination	1565:1593	the N isotopic discrimination occurring before absorption	1565:1621	For both diets, the digestive contribution (i.e. the N isotopic discrimination occurring before absorption) to the Δ15N observed in animal tissues accounted for 65 ± 11%, leaving only one third to the contribution of post-absorptive metabolism.
32502191	13	81	theme	Δ15N	2422:2425	arg1	values					2427:2432	higher (P ≤ 0.05) Δ15N values	2404:2432	higher (P ≤ 0.05) Δ15N values across all post-absorptive metabolic pools	2404:2475	Fiber diet compared to starch diet had a lower feed efficiency and promoted higher (P ≤ 0.05) Δ15N values across all post-absorptive metabolic pools and some digestive pools (ruminal, duodenal, and ileal contents).
32502191	2	82	theme	diets	522:526	arg1	nature					512:517	the nature	508:517	the nature of diets	508:526	However, results from the literature are not always consistent across studies, likely due to isotopic discrimination pathways that may differ with the nature of diets.
32502191	11	83	theme	key	1967:1969	arg1	role					1971:1974	the key role	1963:1974	the key role of the rumen on N isotopic discrimination	1963:2016	Δ15N = 2.24 ± 0.41‰), highlighting the key role of the rumen on N isotopic discrimination.
32502191	9	84	theme	changes	1823:1829	arg1	majority					1805:1812	the majority	1801:1812	the majority of these changes	1801:1829	Concerning the Δ15N in digestive pools, the majority of these changes occurred in the rumen (av.
32502191	13	85	theme	higher	2404:2409	arg1	values					2427:2432	higher (P ≤ 0.05) Δ15N values	2404:2432	higher (P ≤ 0.05) Δ15N values across all post-absorptive metabolic pools	2404:2475	Fiber diet compared to starch diet had a lower feed efficiency and promoted higher (P ≤ 0.05) Δ15N values across all post-absorptive metabolic pools and some digestive pools (ruminal, duodenal, and ileal contents).
32502191	15	86	theme	contrasted	2806:2815	arg1	use					2817:2819	the contrasted use	2802:2819	the contrasted use of N	2802:2824	Our results highlight the contrasted use of N at the rumen level between the two experimental diets and suggests the need for different equations to predict FCE or NUE from Δ15N according to the type of diet.
32502191	0	87	with	relationship	48:59	arg1	efficiency					71:80	feed efficiency	66:80	feed efficiency	66:80	The origin of N isotopic discrimination and its relationship with feed efficiency in fattening yearling bulls is diet-dependent.
32502191	14	88	theme	feed	2598:2601	arg1	efficiency					2603:2612	feed efficiency	2598:2612	feed efficiency	2598:2612	The within-diet relationship (n = 12) between Δ15N and feed efficiency was not as strong and consistent as the overall relationship, with contrasted responses between the two diets for specific pools (diet x pool interaction; P ≤ 0.01).
32502191	7	89	theme	N	1492:1492	arg1	composition					1503:1513	their N isotopic composition	1486:1513	their N isotopic composition	1486:1513	All samples along with feed were analyzed for their N isotopic composition.
32502191	14	90	theme	x	2749:2749	arg1	interaction					2756:2766	diet x pool interaction	2744:2766	diet x pool interaction	2744:2766	The within-diet relationship (n = 12) between Δ15N and feed efficiency was not as strong and consistent as the overall relationship, with contrasted responses between the two diets for specific pools (diet x pool interaction; P ≤ 0.01).
32502191	14	90	theme	x	2749:2749	arg1	pools					2737:2741	specific pools	2728:2741	specific pools (diet x pool interaction; P ≤ 0.01)	2728:2777	The within-diet relationship (n = 12) between Δ15N and feed efficiency was not as strong and consistent as the overall relationship, with contrasted responses between the two diets for specific pools (diet x pool interaction; P ≤ 0.01).
32502191	4	91	from	level	965:969	arg1	ratio					946:950	energy ratio	939:950	energy ratio at the rumen level (starch)	939:978	Twenty-four Charolais yearling bulls were randomly divided into two groups and fed during 8 months, from weaning to slaughter, either 1) a high starch diet based on corn silage supplying a balanced N to energy ratio at the rumen level (starch) or 2) a high fiber diet based on grass silage supplying an excess of rumen degradable N (fiber).
32502191	4	92	theme	degradable	1055:1064	arg1	N					1066:1066	rumen degradable N	1049:1066	rumen degradable N (fiber)	1049:1074	Twenty-four Charolais yearling bulls were randomly divided into two groups and fed during 8 months, from weaning to slaughter, either 1) a high starch diet based on corn silage supplying a balanced N to energy ratio at the rumen level (starch) or 2) a high fiber diet based on grass silage supplying an excess of rumen degradable N (fiber).
32502191	4	92	theme	degradable	1055:1064	arg1	fiber					1069:1073	fiber	1069:1073	fiber	1069:1073	Twenty-four Charolais yearling bulls were randomly divided into two groups and fed during 8 months, from weaning to slaughter, either 1) a high starch diet based on corn silage supplying a balanced N to energy ratio at the rumen level (starch) or 2) a high fiber diet based on grass silage supplying an excess of rumen degradable N (fiber).
32502191	12	93	theme	muscle	2176:2181	arg1	pool					2150:2153	any post-absorptive metabolic pool	2120:2153	any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01)	2120:2237	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	12	93	theme	muscle	2176:2181	arg1	tissues					2183:2189	muscle tissues	2176:2189	muscle tissues	2176:2189	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	16	94	theme	yearling	3224:3231	arg1	bulls					3233:3237	fattening yearling bulls	3214:3237	fattening yearling bulls	3214:3237	In conclusion, rumen digestion and associated microbial activity can play an important role on N isotopic discrimination so rumen effect related to diet may interfere with the relationship between Δ15N and feed efficiency in fattening yearling bulls.
32502191	14	95	theme	=	2575:2575	arg1	relationship					2559:2570	The within-diet relationship	2543:2570	The within-diet relationship (n = 12) between Δ15N	2543:2592	The within-diet relationship (n = 12) between Δ15N and feed efficiency was not as strong and consistent as the overall relationship, with contrasted responses between the two diets for specific pools (diet x pool interaction; P ≤ 0.01).
32502191	14	95	theme	=	2575:2575	arg1	n					2573:2573	n = 12	2573:2578	n = 12	2573:2578	The within-diet relationship (n = 12) between Δ15N and feed efficiency was not as strong and consistent as the overall relationship, with contrasted responses between the two diets for specific pools (diet x pool interaction; P ≤ 0.01).
32502191	1	96	theme	natural	190:196	arg1	abundance					202:210	natural 15N abundance	190:210	natural 15N abundance	190:210	Nitrogen (N) isotopic discrimination (i.e. the difference in natural 15N abundance between the animal proteins and the diet; Δ15N) is known to correlate with N use efficiency (NUE) and feed conversion efficiency (FCE) in ruminants.
32502191	12	97	theme	metabolic	2140:2148	arg1	proteins					2202:2209	plasma proteins	2195:2209	plasma proteins	2195:2209	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	12	97	theme	metabolic	2140:2148	arg1	tissues					2183:2189	muscle tissues	2176:2189	muscle tissues	2176:2189	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	12	97	theme	metabolic	2140:2148	arg1	duodenum					2156:2163	duodenum	2156:2163	duodenum	2156:2163	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	12	97	theme	metabolic	2140:2148	arg1	pool					2150:2153	any post-absorptive metabolic pool	2120:2153	any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01)	2120:2237	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	12	97	theme	metabolic	2140:2148	arg1	liver					2166:2170	liver	2166:2170	liver	2166:2170	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	2	98	theme	isotopic	454:461	arg1	pathways					478:485	isotopic discrimination pathways	454:485	isotopic discrimination pathways that may differ with the nature of diets	454:526	However, results from the literature are not always consistent across studies, likely due to isotopic discrimination pathways that may differ with the nature of diets.
32502191	4	99	theme	high	988:991	arg1	diet					999:1002	a high fiber diet	986:1002	2) a high fiber diet based on grass silage supplying an excess of rumen degradable N (fiber)	983:1074	Twenty-four Charolais yearling bulls were randomly divided into two groups and fed during 8 months, from weaning to slaughter, either 1) a high starch diet based on corn silage supplying a balanced N to energy ratio at the rumen level (starch) or 2) a high fiber diet based on grass silage supplying an excess of rumen degradable N (fiber).
32502191	12	100	theme	overall	2041:2047	arg1	n					2063:2063	n = 24	2063:2068	n = 24	2063:2068	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	12	100	theme	overall	2041:2047	arg1	relationship					2049:2060	A strong, significant overall relationship	2019:2060	relationship	2049:2060	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	9	101	from	Δ15N	1776:1779	arg1	pools					1794:1798	digestive pools	1784:1798	digestive pools	1784:1798	Concerning the Δ15N in digestive pools, the majority of these changes occurred in the rumen (av.
32502191	6	102	theme	liquid-associated	1331:1347	arg1	ammonia					1383:1389	free ammonia	1378:1389	free ammonia	1378:1389	Ruminal content was further used to isolate liquid-associated bacteria (LAB), protozoa and free ammonia, while plasma proteins were obtained from blood.
32502191	6	102	theme	liquid-associated	1331:1347	arg1	LAB					1359:1361	LAB	1359:1361	LAB	1359:1361	Ruminal content was further used to isolate liquid-associated bacteria (LAB), protozoa and free ammonia, while plasma proteins were obtained from blood.
32502191	6	102	theme	liquid-associated	1331:1347	arg1	protozoa					1365:1372	protozoa	1365:1372	protozoa	1365:1372	Ruminal content was further used to isolate liquid-associated bacteria (LAB), protozoa and free ammonia, while plasma proteins were obtained from blood.
32502191	6	102	theme	liquid-associated	1331:1347	arg1	bacteria					1349:1356	liquid-associated bacteria	1331:1356	liquid-associated bacteria (LAB)	1331:1362	Ruminal content was further used to isolate liquid-associated bacteria (LAB), protozoa and free ammonia, while plasma proteins were obtained from blood.
32502191	5	103	dep	duodenum	1210:1217	arg1	muscle					1230:1235	muscle	1230:1235	muscle	1230:1235	All animals were slaughtered and samples of different digestive pools (ruminal, duodenal, ileal and fecal contents), animal tissues (duodenum, liver and muscle), blood and urine were collected for each animal.
32502191	5	103	dep	duodenum	1210:1217	arg1	liver					1220:1224	liver	1220:1224	liver	1220:1224	All animals were slaughtered and samples of different digestive pools (ruminal, duodenal, ileal and fecal contents), animal tissues (duodenum, liver and muscle), blood and urine were collected for each animal.
32502191	14	104	theme	overall	2654:2660	arg1	relationship					2662:2673	the overall relationship	2650:2673	the overall relationship	2650:2673	The within-diet relationship (n = 12) between Δ15N and feed efficiency was not as strong and consistent as the overall relationship, with contrasted responses between the two diets for specific pools (diet x pool interaction; P ≤ 0.01).
32502191	12	105	theme	plasma	2195:2200	arg1	proteins					2202:2209	plasma proteins	2195:2209	plasma proteins	2195:2209	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	12	105	theme	plasma	2195:2200	arg1	pool					2150:2153	any post-absorptive metabolic pool	2120:2153	any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01)	2120:2237	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	8	106	theme	±	1680:1680	arg1	%					1684:1684	65 ± 11%	1677:1684	65 ± 11%	1677:1684	For both diets, the digestive contribution (i.e. the N isotopic discrimination occurring before absorption) to the Δ15N observed in animal tissues accounted for 65 ± 11%, leaving only one third to the contribution of post-absorptive metabolism.
32502191	5	107	dep	tissues	1201:1207	arg1	duodenum					1210:1217	duodenum	1210:1217	duodenum	1210:1217	All animals were slaughtered and samples of different digestive pools (ruminal, duodenal, ileal and fecal contents), animal tissues (duodenum, liver and muscle), blood and urine were collected for each animal.
32502191	3	108	theme	yearling	681:688	arg1	bulls					690:694	fattening yearling bulls	671:694	fattening yearling bulls	671:694	The objective of the present study was to assess at which level, from rumen to tissues, Δ15N originates and becomes related to NUE and FCE in fattening yearling bulls when they are fed two contrasted diets.
32502191	1	109	theme	use	289:291	arg1	efficiency					293:302	N use efficiency	287:302	N use efficiency (NUE)	287:308	Nitrogen (N) isotopic discrimination (i.e. the difference in natural 15N abundance between the animal proteins and the diet; Δ15N) is known to correlate with N use efficiency (NUE) and feed conversion efficiency (FCE) in ruminants.
32502191	1	109	theme	use	289:291	arg1	NUE					305:307	NUE	305:307	NUE	305:307	Nitrogen (N) isotopic discrimination (i.e. the difference in natural 15N abundance between the animal proteins and the diet; Δ15N) is known to correlate with N use efficiency (NUE) and feed conversion efficiency (FCE) in ruminants.
32502191	13	110	theme	lower	2369:2373	arg1	efficiency					2380:2389	a lower feed efficiency	2367:2389	a lower feed efficiency	2367:2389	Fiber diet compared to starch diet had a lower feed efficiency and promoted higher (P ≤ 0.05) Δ15N values across all post-absorptive metabolic pools and some digestive pools (ruminal, duodenal, and ileal contents).
32502191	0	111	from	origin	4:9	arg1	bulls					104:108	fattening yearling bulls	85:108	fattening yearling bulls	85:108	The origin of N isotopic discrimination and its relationship with feed efficiency in fattening yearling bulls is diet-dependent.
32502191	11	112	theme	N	1992:1992	arg1	discrimination					2003:2016	N isotopic discrimination	1992:2016	N isotopic discrimination	1992:2016	Δ15N = 2.24 ± 0.41‰), highlighting the key role of the rumen on N isotopic discrimination.
32502191	0	113	theme	fattening	85:93	arg1	bulls					104:108	fattening yearling bulls	85:108	fattening yearling bulls	85:108	The origin of N isotopic discrimination and its relationship with feed efficiency in fattening yearling bulls is diet-dependent.
32502191	16	114	theme	associated	3024:3033	arg1	activity					3045:3052	associated microbial activity	3024:3052	associated microbial activity	3024:3052	In conclusion, rumen digestion and associated microbial activity can play an important role on N isotopic discrimination so rumen effect related to diet may interfere with the relationship between Δ15N and feed efficiency in fattening yearling bulls.
32502191	6	115	theme	free	1378:1381	arg1	bacteria					1349:1356	liquid-associated bacteria	1331:1356	liquid-associated bacteria (LAB)	1331:1362	Ruminal content was further used to isolate liquid-associated bacteria (LAB), protozoa and free ammonia, while plasma proteins were obtained from blood.
32502191	6	115	theme	free	1378:1381	arg1	ammonia					1383:1389	free ammonia	1378:1389	free ammonia	1378:1389	Ruminal content was further used to isolate liquid-associated bacteria (LAB), protozoa and free ammonia, while plasma proteins were obtained from blood.
32502191	8	116	dep	discrimination	1580:1593	arg1	i.e.					1560:1563	i.e.	1560:1563	i.e.	1560:1563	For both diets, the digestive contribution (i.e. the N isotopic discrimination occurring before absorption) to the Δ15N observed in animal tissues accounted for 65 ± 11%, leaving only one third to the contribution of post-absorptive metabolism.
32502191	2	117	from	literature	387:396	arg1	results					370:376	results	370:376	results from the literature	370:396	However, results from the literature are not always consistent across studies, likely due to isotopic discrimination pathways that may differ with the nature of diets.
32502191	11	118	theme	rumen	1983:1987	arg1	role					1971:1974	the key role	1963:1974	the key role of the rumen on N isotopic discrimination	1963:2016	Δ15N = 2.24 ± 0.41‰), highlighting the key role of the rumen on N isotopic discrimination.
32502191	13	119	theme	starch	2351:2356	arg1	diet					2358:2361	starch diet	2351:2361	starch diet	2351:2361	Fiber diet compared to starch diet had a lower feed efficiency and promoted higher (P ≤ 0.05) Δ15N values across all post-absorptive metabolic pools and some digestive pools (ruminal, duodenal, and ileal contents).
32502191	16	120	theme	important	3066:3074	arg1	role					3076:3079	an important role	3063:3079	an important role	3063:3079	In conclusion, rumen digestion and associated microbial activity can play an important role on N isotopic discrimination so rumen effect related to diet may interfere with the relationship between Δ15N and feed efficiency in fattening yearling bulls.
32502191	5	121	theme	different	1121:1129	arg1	tissues					1201:1207	animal tissues	1194:1207	animal tissues (duodenum, liver and muscle)	1194:1236	All animals were slaughtered and samples of different digestive pools (ruminal, duodenal, ileal and fecal contents), animal tissues (duodenum, liver and muscle), blood and urine were collected for each animal.
32502191	5	121	theme	different	1121:1129	arg1	pools					1141:1145	different digestive pools	1121:1145	different digestive pools (ruminal, duodenal, ileal and fecal contents)	1121:1191	All animals were slaughtered and samples of different digestive pools (ruminal, duodenal, ileal and fecal contents), animal tissues (duodenum, liver and muscle), blood and urine were collected for each animal.
32502191	5	121	theme	different	1121:1129	arg1	blood					1239:1243	blood	1239:1243	blood	1239:1243	All animals were slaughtered and samples of different digestive pools (ruminal, duodenal, ileal and fecal contents), animal tissues (duodenum, liver and muscle), blood and urine were collected for each animal.
32502191	5	121	theme	different	1121:1129	arg1	urine					1249:1253	urine	1249:1253	urine	1249:1253	All animals were slaughtered and samples of different digestive pools (ruminal, duodenal, ileal and fecal contents), animal tissues (duodenum, liver and muscle), blood and urine were collected for each animal.
32502191	5	121	theme	different	1121:1129	arg1	contents					1183:1190	ruminal, duodenal, ileal and fecal contents	1148:1190	ruminal, duodenal, ileal and fecal contents	1148:1190	All animals were slaughtered and samples of different digestive pools (ruminal, duodenal, ileal and fecal contents), animal tissues (duodenum, liver and muscle), blood and urine were collected for each animal.
32502191	1	122	theme	feed	314:317	arg1	FCE					342:344	FCE	342:344	FCE	342:344	Nitrogen (N) isotopic discrimination (i.e. the difference in natural 15N abundance between the animal proteins and the diet; Δ15N) is known to correlate with N use efficiency (NUE) and feed conversion efficiency (FCE) in ruminants.
32502191	1	122	theme	feed	314:317	arg1	efficiency					330:339	feed conversion efficiency	314:339	feed conversion efficiency (FCE) in ruminants	314:358	Nitrogen (N) isotopic discrimination (i.e. the difference in natural 15N abundance between the animal proteins and the diet; Δ15N) is known to correlate with N use efficiency (NUE) and feed conversion efficiency (FCE) in ruminants.
32502191	4	123	theme	high	875:878	arg1	diet					887:890	a high starch diet	873:890	a high starch diet based on corn silage supplying a balanced N to energy ratio at the rumen level (starch)	873:978	Twenty-four Charolais yearling bulls were randomly divided into two groups and fed during 8 months, from weaning to slaughter, either 1) a high starch diet based on corn silage supplying a balanced N to energy ratio at the rumen level (starch) or 2) a high fiber diet based on grass silage supplying an excess of rumen degradable N (fiber).
32502191	10	124	theme	15N	1896:1898	arg1	enrichments					1900:1910	only minor 15N enrichments	1885:1910	only minor 15N enrichments	1885:1910	Δ15N = 2.12 ± 0.66‰), with only minor 15N enrichments thereafter (av.
32502191	0	125	theme	N	14:14	arg1	discrimination					25:38	N isotopic discrimination	14:38	N isotopic discrimination	14:38	The origin of N isotopic discrimination and its relationship with feed efficiency in fattening yearling bulls is diet-dependent.
32502191	16	126	theme	rumen	3113:3117	arg1	effect					3119:3124	rumen effect	3113:3124	rumen effect related to diet	3113:3140	In conclusion, rumen digestion and associated microbial activity can play an important role on N isotopic discrimination so rumen effect related to diet may interfere with the relationship between Δ15N and feed efficiency in fattening yearling bulls.
32502191	13	127	theme	Fiber	2328:2332	arg1	diet					2334:2337	Fiber diet	2328:2337	Fiber diet compared to starch diet	2328:2361	Fiber diet compared to starch diet had a lower feed efficiency and promoted higher (P ≤ 0.05) Δ15N values across all post-absorptive metabolic pools and some digestive pools (ruminal, duodenal, and ileal contents).
32502191	16	128	theme	isotopic	3086:3093	arg1	discrimination					3095:3108	N isotopic discrimination	3084:3108	N isotopic discrimination so rumen effect related to diet may interfere with the relationship between Δ15N and feed efficiency in fattening yearling bulls	3084:3237	In conclusion, rumen digestion and associated microbial activity can play an important role on N isotopic discrimination so rumen effect related to diet may interfere with the relationship between Δ15N and feed efficiency in fattening yearling bulls.
32502191	0	129	theme	discrimination	25:38	arg1	origin					4:9	The origin	0:9	The origin of N isotopic discrimination and its relationship with feed efficiency in fattening yearling bulls	0:108	The origin of N isotopic discrimination and its relationship with feed efficiency in fattening yearling bulls is diet-dependent.
32502191	0	129	theme	discrimination	25:38	arg1	diet-dependent					113:126	diet-dependent	113:126	diet-dependent	113:126	The origin of N isotopic discrimination and its relationship with feed efficiency in fattening yearling bulls is diet-dependent.
32502191	16	130	theme	related	3126:3132	arg1	effect					3119:3124	rumen effect	3113:3124	rumen effect related to diet	3113:3140	In conclusion, rumen digestion and associated microbial activity can play an important role on N isotopic discrimination so rumen effect related to diet may interfere with the relationship between Δ15N and feed efficiency in fattening yearling bulls.
32502191	12	131	theme	metabolic	2307:2315	arg1	phenomena					2317:2325	both digestive and post-absorptive metabolic phenomena	2272:2325	both digestive and post-absorptive metabolic phenomena	2272:2325	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	12	132	theme	significant	2029:2039	arg1	n					2063:2063	n = 24	2063:2068	n = 24	2063:2068	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	12	132	theme	significant	2029:2039	arg1	relationship					2049:2060	A strong, significant overall relationship	2019:2060	relationship	2049:2060	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	3	133	theme	present	550:556	arg1	study					558:562	the present study	546:562	the present study	546:562	The objective of the present study was to assess at which level, from rumen to tissues, Δ15N originates and becomes related to NUE and FCE in fattening yearling bulls when they are fed two contrasted diets.
32502191	5	134	theme	duodenal	1157:1164	arg1	pools					1141:1145	different digestive pools	1121:1145	different digestive pools (ruminal, duodenal, ileal and fecal contents)	1121:1191	All animals were slaughtered and samples of different digestive pools (ruminal, duodenal, ileal and fecal contents), animal tissues (duodenum, liver and muscle), blood and urine were collected for each animal.
32502191	5	134	theme	duodenal	1157:1164	arg1	contents					1183:1190	ruminal, duodenal, ileal and fecal contents	1148:1190	ruminal, duodenal, ileal and fecal contents	1148:1190	All animals were slaughtered and samples of different digestive pools (ruminal, duodenal, ileal and fecal contents), animal tissues (duodenum, liver and muscle), blood and urine were collected for each animal.
32502191	1	135	from	efficiency	330:339	arg1	ruminants					350:358	ruminants	350:358	ruminants	350:358	Nitrogen (N) isotopic discrimination (i.e. the difference in natural 15N abundance between the animal proteins and the diet; Δ15N) is known to correlate with N use efficiency (NUE) and feed conversion efficiency (FCE) in ruminants.
32502191	13	136	theme	digestive	2486:2494	arg1	ruminal					2503:2509	ruminal	2503:2509	ruminal	2503:2509	Fiber diet compared to starch diet had a lower feed efficiency and promoted higher (P ≤ 0.05) Δ15N values across all post-absorptive metabolic pools and some digestive pools (ruminal, duodenal, and ileal contents).
32502191	13	136	theme	digestive	2486:2494	arg1	pools					2496:2500	some digestive pools	2481:2500	some digestive pools (ruminal, duodenal, and ileal contents)	2481:2540	Fiber diet compared to starch diet had a lower feed efficiency and promoted higher (P ≤ 0.05) Δ15N values across all post-absorptive metabolic pools and some digestive pools (ruminal, duodenal, and ileal contents).
32502191	13	136	theme	digestive	2486:2494	arg1	duodenal					2512:2519	duodenal	2512:2519	duodenal	2512:2519	Fiber diet compared to starch diet had a lower feed efficiency and promoted higher (P ≤ 0.05) Δ15N values across all post-absorptive metabolic pools and some digestive pools (ruminal, duodenal, and ileal contents).
32502191	8	137	theme	post-absorptive	1733:1747	arg1	metabolism					1749:1758	post-absorptive metabolism	1733:1758	post-absorptive metabolism	1733:1758	For both diets, the digestive contribution (i.e. the N isotopic discrimination occurring before absorption) to the Δ15N observed in animal tissues accounted for 65 ± 11%, leaving only one third to the contribution of post-absorptive metabolism.
32502191	9	138	theme	digestive	1784:1792	arg1	pools					1794:1798	digestive pools	1784:1798	digestive pools	1784:1798	Concerning the Δ15N in digestive pools, the majority of these changes occurred in the rumen (av.
32502191	5	139	theme	ileal	1167:1171	arg1	pools					1141:1145	different digestive pools	1121:1145	different digestive pools (ruminal, duodenal, ileal and fecal contents)	1121:1191	All animals were slaughtered and samples of different digestive pools (ruminal, duodenal, ileal and fecal contents), animal tissues (duodenum, liver and muscle), blood and urine were collected for each animal.
32502191	5	139	theme	ileal	1167:1171	arg1	contents					1183:1190	ruminal, duodenal, ileal and fecal contents	1148:1190	ruminal, duodenal, ileal and fecal contents	1148:1190	All animals were slaughtered and samples of different digestive pools (ruminal, duodenal, ileal and fecal contents), animal tissues (duodenum, liver and muscle), blood and urine were collected for each animal.
32502191	8	140	located	observed	1636:1643	arg1	tissues					1655:1661	animal tissues	1648:1661	animal tissues	1648:1661	For both diets, the digestive contribution (i.e. the N isotopic discrimination occurring before absorption) to the Δ15N observed in animal tissues accounted for 65 ± 11%, leaving only one third to the contribution of post-absorptive metabolism.
32502191	8	140	located	observed	1636:1643	arg2	Δ15N					1631:1634	the Δ15N	1627:1634	the Δ15N observed in animal tissues	1627:1661	For both diets, the digestive contribution (i.e. the N isotopic discrimination occurring before absorption) to the Δ15N observed in animal tissues accounted for 65 ± 11%, leaving only one third to the contribution of post-absorptive metabolism.
32502191	0	141	with	discrimination	25:38	arg1	efficiency					71:80	feed efficiency	66:80	feed efficiency	66:80	The origin of N isotopic discrimination and its relationship with feed efficiency in fattening yearling bulls is diet-dependent.
32502191	13	142	dep	pools	2496:2500	arg1	ruminal					2503:2509	ruminal	2503:2509	ruminal	2503:2509	Fiber diet compared to starch diet had a lower feed efficiency and promoted higher (P ≤ 0.05) Δ15N values across all post-absorptive metabolic pools and some digestive pools (ruminal, duodenal, and ileal contents).
32502191	13	142	dep	pools	2496:2500	arg1	contents					2532:2539	ileal contents	2526:2539	ileal contents	2526:2539	Fiber diet compared to starch diet had a lower feed efficiency and promoted higher (P ≤ 0.05) Δ15N values across all post-absorptive metabolic pools and some digestive pools (ruminal, duodenal, and ileal contents).
32502191	13	142	dep	pools	2496:2500	arg1	pools					2496:2500	some digestive pools	2481:2500	some digestive pools (ruminal, duodenal, and ileal contents)	2481:2540	Fiber diet compared to starch diet had a lower feed efficiency and promoted higher (P ≤ 0.05) Δ15N values across all post-absorptive metabolic pools and some digestive pools (ruminal, duodenal, and ileal contents).
32502191	13	142	dep	pools	2496:2500	arg1	duodenal					2512:2519	duodenal	2512:2519	duodenal	2512:2519	Fiber diet compared to starch diet had a lower feed efficiency and promoted higher (P ≤ 0.05) Δ15N values across all post-absorptive metabolic pools and some digestive pools (ruminal, duodenal, and ileal contents).
32502191	12	143	theme	<	2217:2217	arg1	<					2221:2221	0.52 < r < 0.73	2212:2226	duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73	2156:2226	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	5	144	theme	pools	1141:1145	arg1	samples					1110:1116	samples	1110:1116	samples of different digestive pools (ruminal, duodenal, ileal and fecal contents), animal tissues (duodenum, liver and muscle), blood and urine	1110:1253	All animals were slaughtered and samples of different digestive pools (ruminal, duodenal, ileal and fecal contents), animal tissues (duodenum, liver and muscle), blood and urine were collected for each animal.
32502191	13	145	theme	metabolic	2461:2469	arg1	pools					2471:2475	all post-absorptive metabolic pools	2441:2475	all post-absorptive metabolic pools	2441:2475	Fiber diet compared to starch diet had a lower feed efficiency and promoted higher (P ≤ 0.05) Δ15N values across all post-absorptive metabolic pools and some digestive pools (ruminal, duodenal, and ileal contents).
32502191	12	146	theme	strong	2021:2026	arg1	n					2063:2063	n = 24	2063:2068	n = 24	2063:2068	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	12	146	theme	strong	2021:2026	arg1	relationship					2049:2060	A strong, significant overall relationship	2019:2060	relationship	2049:2060	A strong, significant overall relationship (n = 24) between Δ15N and FCE or NUE was found when using any post-absorptive metabolic pool (duodenum, liver, or muscle tissues, or plasma proteins; 0.52 < r < 0.73; P ≤ 0.01), probably as these pools reflect both digestive and post-absorptive metabolic phenomena.
32502191	5	147	theme	ruminal	1148:1154	arg1	pools					1141:1145	different digestive pools	1121:1145	different digestive pools (ruminal, duodenal, ileal and fecal contents)	1121:1191	All animals were slaughtered and samples of different digestive pools (ruminal, duodenal, ileal and fecal contents), animal tissues (duodenum, liver and muscle), blood and urine were collected for each animal.
32502191	5	147	theme	ruminal	1148:1154	arg1	contents					1183:1190	ruminal, duodenal, ileal and fecal contents	1148:1190	ruminal, duodenal, ileal and fecal contents	1148:1190	All animals were slaughtered and samples of different digestive pools (ruminal, duodenal, ileal and fecal contents), animal tissues (duodenum, liver and muscle), blood and urine were collected for each animal.
32502191	1	148	theme	animal	224:229	arg1	proteins					231:238	the animal proteins	220:238	the animal proteins	220:238	Nitrogen (N) isotopic discrimination (i.e. the difference in natural 15N abundance between the animal proteins and the diet; Δ15N) is known to correlate with N use efficiency (NUE) and feed conversion efficiency (FCE) in ruminants.
33156477	6	0	dep	hyperalgesia	942:953	arg1	model					955:959	model	955:959	the LPS-induced thermal hyperalgesia model	918:959	Moreover, the in vivo antinociceptive effect was assayed using the formalin test and the LPS-induced thermal hyperalgesia model.
33156477	6	1	theme	in	847:848	arg1	effect					871:876	the in vivo antinociceptive effect	843:876	the in vivo antinociceptive effect	843:876	Moreover, the in vivo antinociceptive effect was assayed using the formalin test and the LPS-induced thermal hyperalgesia model.
33156477	9	2	theme	tumour	1365:1370	arg1	TNF-α					1395:1399	TNF-α	1395:1399	TNF-α	1395:1399	The oral (p.o.) treatment with the EFDf at a dose of 3 mg/kg significantly attenuated the paw oedema, polymorphonuclear leukocyte migration, iNOS expression, and tumour necrosis factor alpha (TNF-α) production.
33156477	9	2	theme	tumour	1365:1370	arg1	alpha					1388:1392	tumour necrosis factor alpha	1365:1392	tumour necrosis factor alpha (TNF-α)	1365:1400	The oral (p.o.) treatment with the EFDf at a dose of 3 mg/kg significantly attenuated the paw oedema, polymorphonuclear leukocyte migration, iNOS expression, and tumour necrosis factor alpha (TNF-α) production.
33156477	6	3	theme	thermal	934:940	arg1	hyperalgesia					942:953	the LPS-induced thermal hyperalgesia	918:953	the LPS-induced thermal hyperalgesia model	918:959	Moreover, the in vivo antinociceptive effect was assayed using the formalin test and the LPS-induced thermal hyperalgesia model.
33156477	9	4	theme	factor	1381:1386	arg1	TNF-α					1395:1399	TNF-α	1395:1399	TNF-α	1395:1399	The oral (p.o.) treatment with the EFDf at a dose of 3 mg/kg significantly attenuated the paw oedema, polymorphonuclear leukocyte migration, iNOS expression, and tumour necrosis factor alpha (TNF-α) production.
33156477	9	4	theme	factor	1381:1386	arg1	alpha					1388:1392	tumour necrosis factor alpha	1365:1392	tumour necrosis factor alpha (TNF-α)	1365:1400	The oral (p.o.) treatment with the EFDf at a dose of 3 mg/kg significantly attenuated the paw oedema, polymorphonuclear leukocyte migration, iNOS expression, and tumour necrosis factor alpha (TNF-α) production.
33156477	10	5	theme	test	1484:1487	arg1	phases					1461:1466	both phases	1456:1466	both phases of the formalin test	1456:1487	The EFDf (10 and 30 mg/kg) also inhibited both phases of the formalin test and caused a significant increase in the reaction time in the LPS-induced thermal hyperalgesia model.
33156477	1	6	theme	oil	212:214	arg1	activities					188:197	the in vivo anti-inflammatory and antinociceptive activities	138:197	the in vivo anti-inflammatory and antinociceptive activities of essential oil of the underground stem bark of Duguetia furfuracea, termed EODf	138:279	A previous study reported the in vivo anti-inflammatory and antinociceptive activities of essential oil of the underground stem bark of Duguetia furfuracea, termed EODf.
33156477	9	7	from	dose	1248:1251	arg1	treatment					1219:1227	The oral (p.o.) treatment	1203:1227	The oral (p.o.) treatment with the EFDf at a dose of 3 mg/kg	1203:1262	The oral (p.o.) treatment with the EFDf at a dose of 3 mg/kg significantly attenuated the paw oedema, polymorphonuclear leukocyte migration, iNOS expression, and tumour necrosis factor alpha (TNF-α) production.
33156477	9	7	from	dose	1248:1251	arg1	p.o.					1213:1216	p.o.	1213:1216	p.o.	1213:1216	The oral (p.o.) treatment with the EFDf at a dose of 3 mg/kg significantly attenuated the paw oedema, polymorphonuclear leukocyte migration, iNOS expression, and tumour necrosis factor alpha (TNF-α) production.
33156477	2	8	from	oil	385:387	arg1	fraction					336:343	a phenylpropanoid-enriched fraction	309:343	a phenylpropanoid-enriched fraction from the D. furfuracea (EFDf) essential oil	309:387	This study aimed to obtain a phenylpropanoid-enriched fraction from the D. furfuracea (EFDf) essential oil and to investigate its anti-inflammatory and antinociceptive effects.
33156477	10	9	theme	significant	1502:1512	arg1	increase					1514:1521	a significant increase	1500:1521	a significant increase in the reaction time in the LPS-induced thermal hyperalgesia model	1500:1588	The EFDf (10 and 30 mg/kg) also inhibited both phases of the formalin test and caused a significant increase in the reaction time in the LPS-induced thermal hyperalgesia model.
33156477	7	10	theme	main	1073:1076	arg1	constituents					1078:1089	the main constituents	1069:1089	the main constituents	1069:1089	In the EFDf, 8 major compounds were identified, with α-asarone (36.4%) and 2,4,5-trimethoxystyrene (27.8%) the main constituents.
33156477	2	11	theme	phenylpropanoid-enriched	311:334	arg1	fraction					336:343	a phenylpropanoid-enriched fraction	309:343	a phenylpropanoid-enriched fraction from the D. furfuracea (EFDf) essential oil	309:387	This study aimed to obtain a phenylpropanoid-enriched fraction from the D. furfuracea (EFDf) essential oil and to investigate its anti-inflammatory and antinociceptive effects.
33156477	1	12	theme	stem	235:238	arg1	bark					240:243	the underground stem bark	219:243	the underground stem bark	219:243	A previous study reported the in vivo anti-inflammatory and antinociceptive activities of essential oil of the underground stem bark of Duguetia furfuracea, termed EODf.
33156477	3	13	theme	chemical	463:470	arg1	composition					472:482	The chemical composition	459:482	The chemical composition of the EFDf	459:494	The chemical composition of the EFDf was determined by gas chromatography-mass spectrometry (GC-MS).
33156477	5	14	theme	nitric	760:765	arg1	iNOS					783:786	iNOS	783:786	iNOS	783:786	The effects of the EFDf on the polymorphonuclear leukocyte recruitment and the inducible nitric oxide synthase (iNOS) expression were evaluated in mice footpads.
33156477	5	14	theme	nitric	760:765	arg1	synthase					773:780	inducible nitric oxide synthase	750:780	the inducible nitric oxide synthase (iNOS) expression	746:798	The effects of the EFDf on the polymorphonuclear leukocyte recruitment and the inducible nitric oxide synthase (iNOS) expression were evaluated in mice footpads.
33156477	9	15	theme	oral	1207:1210	arg1	treatment					1219:1227	The oral (p.o.) treatment	1203:1227	The oral (p.o.) treatment with the EFDf at a dose of 3 mg/kg	1203:1262	The oral (p.o.) treatment with the EFDf at a dose of 3 mg/kg significantly attenuated the paw oedema, polymorphonuclear leukocyte migration, iNOS expression, and tumour necrosis factor alpha (TNF-α) production.
33156477	9	15	theme	oral	1207:1210	arg1	p.o.					1213:1216	p.o.	1213:1216	p.o.	1213:1216	The oral (p.o.) treatment with the EFDf at a dose of 3 mg/kg significantly attenuated the paw oedema, polymorphonuclear leukocyte migration, iNOS expression, and tumour necrosis factor alpha (TNF-α) production.
33156477	10	16	theme	reaction	1530:1537	arg1	time					1539:1542	the reaction time	1526:1542	the reaction time in the LPS-induced thermal hyperalgesia model	1526:1588	The EFDf (10 and 30 mg/kg) also inhibited both phases of the formalin test and caused a significant increase in the reaction time in the LPS-induced thermal hyperalgesia model.
33156477	5	17	theme	polymorphonuclear	702:718	arg1	recruitment					730:740	the polymorphonuclear leukocyte recruitment	698:740	the polymorphonuclear leukocyte recruitment	698:740	The effects of the EFDf on the polymorphonuclear leukocyte recruitment and the inducible nitric oxide synthase (iNOS) expression were evaluated in mice footpads.
33156477	1	18	theme	in	142:143	arg1	activities					188:197	the in vivo anti-inflammatory and antinociceptive activities	138:197	the in vivo anti-inflammatory and antinociceptive activities of essential oil of the underground stem bark of Duguetia furfuracea, termed EODf	138:279	A previous study reported the in vivo anti-inflammatory and antinociceptive activities of essential oil of the underground stem bark of Duguetia furfuracea, termed EODf.
33156477	1	19	theme	anti-inflammatory	150:166	arg1	activities					188:197	the in vivo anti-inflammatory and antinociceptive activities	138:197	the in vivo anti-inflammatory and antinociceptive activities of essential oil of the underground stem bark of Duguetia furfuracea, termed EODf	138:279	A previous study reported the in vivo anti-inflammatory and antinociceptive activities of essential oil of the underground stem bark of Duguetia furfuracea, termed EODf.
33156477	11	20	theme	coordination	1658:1669	arg1	alteration					1638:1647	any alteration	1634:1647	any alteration of motor coordination	1634:1669	Finally, EFDf-treated animals did not show any alteration of motor coordination.
33156477	1	21	theme	antinociceptive	172:186	arg1	activities					188:197	the in vivo anti-inflammatory and antinociceptive activities	138:197	the in vivo anti-inflammatory and antinociceptive activities of essential oil of the underground stem bark of Duguetia furfuracea, termed EODf	138:279	A previous study reported the in vivo anti-inflammatory and antinociceptive activities of essential oil of the underground stem bark of Duguetia furfuracea, termed EODf.
33156477	6	22	theme	LPS-induced	922:932	arg1	hyperalgesia					942:953	the LPS-induced thermal hyperalgesia	918:953	the LPS-induced thermal hyperalgesia model	918:959	Moreover, the in vivo antinociceptive effect was assayed using the formalin test and the LPS-induced thermal hyperalgesia model.
33156477	0	23	theme	furfuracea	100:109	arg1	fraction					79:86	a phenylpropanoid-enriched fraction	52:86	a phenylpropanoid-enriched fraction of Duguetia furfuracea	52:109	Anti-inflammatory and antinociceptive activities of a phenylpropanoid-enriched fraction of Duguetia furfuracea.
33156477	4	24	dep	in	564:565	arg1	vivo					567:570	vivo	567:570	vivo	567:570	The in vivo anti-inflammatory activity was evaluated with a lipopolysaccharide (LPS)-induced paw oedema model.
33156477	2	25	dep	essential	375:383	arg1	D.					354:355	D.	354:355	D.	354:355	This study aimed to obtain a phenylpropanoid-enriched fraction from the D. furfuracea (EFDf) essential oil and to investigate its anti-inflammatory and antinociceptive effects.
33156477	5	26	theme	synthase	773:780	arg1	expression					789:798	the inducible nitric oxide synthase (iNOS) expression	746:798	the inducible nitric oxide synthase (iNOS) expression	746:798	The effects of the EFDf on the polymorphonuclear leukocyte recruitment and the inducible nitric oxide synthase (iNOS) expression were evaluated in mice footpads.
33156477	2	27	theme	anti-inflammatory	412:428	arg1	effects					450:456	its anti-inflammatory and antinociceptive effects	408:456	its anti-inflammatory and antinociceptive effects	408:456	This study aimed to obtain a phenylpropanoid-enriched fraction from the D. furfuracea (EFDf) essential oil and to investigate its anti-inflammatory and antinociceptive effects.
33156477	9	28	theme	3 mg/kg	1256:1262	arg1	dose					1248:1251	a dose	1246:1251	a dose of 3 mg/kg	1246:1262	The oral (p.o.) treatment with the EFDf at a dose of 3 mg/kg significantly attenuated the paw oedema, polymorphonuclear leukocyte migration, iNOS expression, and tumour necrosis factor alpha (TNF-α) production.
33156477	13	29	from	contrast	1830:1837	arg1	similar					1885:1891	similar	1885:1891	similar	1885:1891	In contrast, the antinociception promoted by the EFDf was similar to the EODf and was mediated via activation of adenosinergic and opioidergic receptors.
33156477	13	29	from	contrast	1830:1837	arg1	antinociception					1844:1858	the antinociception	1840:1858	the antinociception promoted by the EFDf	1840:1879	In contrast, the antinociception promoted by the EFDf was similar to the EODf and was mediated via activation of adenosinergic and opioidergic receptors.
33156477	8	30	dep	found	1150:1154	arg1	compared					1175:1182	compared	1175:1182	compared to the EODf (38%)	1175:1200	A higher concentration of phenylpropanoid derivatives was found in the EFDf, 64.2% compared to the EODf (38%).
33156477	10	31	theme	thermal	1563:1569	arg1	hyperalgesia					1571:1582	the LPS-induced thermal hyperalgesia	1547:1582	the LPS-induced thermal hyperalgesia model	1547:1588	The EFDf (10 and 30 mg/kg) also inhibited both phases of the formalin test and caused a significant increase in the reaction time in the LPS-induced thermal hyperalgesia model.
33156477	0	32	theme	Anti-inflammatory	0:16	arg1	activities					38:47	Anti-inflammatory and antinociceptive activities	0:47	Anti-inflammatory and antinociceptive activities of a phenylpropanoid-enriched fraction of Duguetia furfuracea.	0:110	Anti-inflammatory and antinociceptive activities of a phenylpropanoid-enriched fraction of Duguetia furfuracea.
33156477	1	33	theme	previous	114:121	arg1	study					123:127	A previous study	112:127	A previous study	112:127	A previous study reported the in vivo anti-inflammatory and antinociceptive activities of essential oil of the underground stem bark of Duguetia furfuracea, termed EODf.
33156477	0	34	theme	antinociceptive	22:36	arg1	activities					38:47	Anti-inflammatory and antinociceptive activities	0:47	Anti-inflammatory and antinociceptive activities of a phenylpropanoid-enriched fraction of Duguetia furfuracea.	0:110	Anti-inflammatory and antinociceptive activities of a phenylpropanoid-enriched fraction of Duguetia furfuracea.
33156477	9	35	theme	paw	1293:1295	arg1	oedema					1297:1302	paw oedema	1293:1302	paw oedema	1293:1302	The oral (p.o.) treatment with the EFDf at a dose of 3 mg/kg significantly attenuated the paw oedema, polymorphonuclear leukocyte migration, iNOS expression, and tumour necrosis factor alpha (TNF-α) production.
33156477	6	36	theme	formalin	900:907	arg1	test					909:912	the formalin test	896:912	the formalin test	896:912	Moreover, the in vivo antinociceptive effect was assayed using the formalin test and the LPS-induced thermal hyperalgesia model.
33156477	7	37	dep	α-asarone	1015:1023	arg1	constituents					1078:1089	the main constituents	1069:1089	the main constituents	1069:1089	In the EFDf, 8 major compounds were identified, with α-asarone (36.4%) and 2,4,5-trimethoxystyrene (27.8%) the main constituents.
33156477	2	38	theme	antinociceptive	434:448	arg1	effects					450:456	its anti-inflammatory and antinociceptive effects	408:456	its anti-inflammatory and antinociceptive effects	408:456	This study aimed to obtain a phenylpropanoid-enriched fraction from the D. furfuracea (EFDf) essential oil and to investigate its anti-inflammatory and antinociceptive effects.
33156477	5	39	theme	mice	818:821	arg1	footpads					823:830	mice footpads	818:830	mice footpads	818:830	The effects of the EFDf on the polymorphonuclear leukocyte recruitment and the inducible nitric oxide synthase (iNOS) expression were evaluated in mice footpads.
33156477	3	40	theme	chromatography-mass	518:536	arg1	GC-MS					552:556	GC-MS	552:556	GC-MS	552:556	The chemical composition of the EFDf was determined by gas chromatography-mass spectrometry (GC-MS).
33156477	3	40	theme	chromatography-mass	518:536	arg1	spectrometry					538:549	gas chromatography-mass spectrometry	514:549	gas chromatography-mass spectrometry (GC-MS)	514:557	The chemical composition of the EFDf was determined by gas chromatography-mass spectrometry (GC-MS).
33156477	0	41	theme	phenylpropanoid-enriched	54:77	arg1	fraction					79:86	a phenylpropanoid-enriched fraction	52:86	a phenylpropanoid-enriched fraction of Duguetia furfuracea	52:109	Anti-inflammatory and antinociceptive activities of a phenylpropanoid-enriched fraction of Duguetia furfuracea.
33156477	9	42	theme	leukocyte	1323:1331	arg1	migration					1333:1341	polymorphonuclear leukocyte migration	1305:1341	polymorphonuclear leukocyte migration	1305:1341	The oral (p.o.) treatment with the EFDf at a dose of 3 mg/kg significantly attenuated the paw oedema, polymorphonuclear leukocyte migration, iNOS expression, and tumour necrosis factor alpha (TNF-α) production.
33156477	11	43	theme	motor	1652:1656	arg1	coordination					1658:1669	motor coordination	1652:1669	motor coordination	1652:1669	Finally, EFDf-treated animals did not show any alteration of motor coordination.
33156477	13	44	theme	receptors	1970:1978	arg1	activation					1926:1935	activation	1926:1935	activation of adenosinergic and opioidergic receptors	1926:1978	In contrast, the antinociception promoted by the EFDf was similar to the EODf and was mediated via activation of adenosinergic and opioidergic receptors.
33156477	1	45	theme	Duguetia	248:255	arg1	furfuracea					257:266	Duguetia furfuracea	248:266	Duguetia furfuracea	248:266	A previous study reported the in vivo anti-inflammatory and antinociceptive activities of essential oil of the underground stem bark of Duguetia furfuracea, termed EODf.
33156477	8	46	theme	phenylpropanoid	1118:1132	arg1	derivatives					1134:1144	phenylpropanoid derivatives	1118:1144	phenylpropanoid derivatives	1118:1144	A higher concentration of phenylpropanoid derivatives was found in the EFDf, 64.2% compared to the EODf (38%).
33156477	6	47	theme	antinociceptive	855:869	arg1	effect					871:876	the in vivo antinociceptive effect	843:876	the in vivo antinociceptive effect	843:876	Moreover, the in vivo antinociceptive effect was assayed using the formalin test and the LPS-induced thermal hyperalgesia model.
33156477	12	48	theme	2,4,5-trimethoxystyrene	1715:1737	arg1	enrichment					1701:1710	the enrichment	1697:1710	the enrichment of 2,4,5-trimethoxystyrene and α-asarone	1697:1751	The results suggest that the enrichment of 2,4,5-trimethoxystyrene and α-asarone enhances the anti-inflammatory activity of the EFDf compared to the EODf.
33156477	10	49	theme	formalin	1475:1482	arg1	test					1484:1487	the formalin test	1471:1487	the formalin test	1471:1487	The EFDf (10 and 30 mg/kg) also inhibited both phases of the formalin test and caused a significant increase in the reaction time in the LPS-induced thermal hyperalgesia model.
33156477	9	50	theme	expression	1349:1358	arg1	production					1402:1411	the paw oedema, polymorphonuclear leukocyte migration, iNOS expression, and tumour necrosis factor alpha (TNF-α) production	1289:1411	the paw oedema, polymorphonuclear leukocyte migration, iNOS expression, and tumour necrosis factor alpha (TNF-α) production	1289:1411	The oral (p.o.) treatment with the EFDf at a dose of 3 mg/kg significantly attenuated the paw oedema, polymorphonuclear leukocyte migration, iNOS expression, and tumour necrosis factor alpha (TNF-α) production.
33156477	10	51	from	increase	1514:1521	arg1	time					1539:1542	the reaction time	1526:1542	the reaction time in the LPS-induced thermal hyperalgesia model	1526:1588	The EFDf (10 and 30 mg/kg) also inhibited both phases of the formalin test and caused a significant increase in the reaction time in the LPS-induced thermal hyperalgesia model.
33156477	12	52	theme	α-asarone	1743:1751	arg1	enrichment					1701:1710	the enrichment	1697:1710	the enrichment of 2,4,5-trimethoxystyrene and α-asarone	1697:1751	The results suggest that the enrichment of 2,4,5-trimethoxystyrene and α-asarone enhances the anti-inflammatory activity of the EFDf compared to the EODf.
33156477	4	53	theme	paw	653:655	arg1	model					664:668	a lipopolysaccharide (LPS)-induced paw oedema model	618:668	a lipopolysaccharide (LPS)-induced paw oedema model	618:668	The in vivo anti-inflammatory activity was evaluated with a lipopolysaccharide (LPS)-induced paw oedema model.
33156477	2	54	theme	essential	375:383	arg1	oil					385:387	the D. furfuracea (EFDf) essential oil	350:387	the D. furfuracea (EFDf) essential oil	350:387	This study aimed to obtain a phenylpropanoid-enriched fraction from the D. furfuracea (EFDf) essential oil and to investigate its anti-inflammatory and antinociceptive effects.
33156477	5	55	theme	EFDf	690:693	arg1	effects					675:681	The effects	671:681	The effects of the EFDf on the polymorphonuclear leukocyte recruitment and the inducible nitric oxide synthase (iNOS) expression	671:798	The effects of the EFDf on the polymorphonuclear leukocyte recruitment and the inducible nitric oxide synthase (iNOS) expression were evaluated in mice footpads.
33156477	9	56	theme	necrosis	1372:1379	arg1	TNF-α					1395:1399	TNF-α	1395:1399	TNF-α	1395:1399	The oral (p.o.) treatment with the EFDf at a dose of 3 mg/kg significantly attenuated the paw oedema, polymorphonuclear leukocyte migration, iNOS expression, and tumour necrosis factor alpha (TNF-α) production.
33156477	9	56	theme	necrosis	1372:1379	arg1	alpha					1388:1392	tumour necrosis factor alpha	1365:1392	tumour necrosis factor alpha (TNF-α)	1365:1400	The oral (p.o.) treatment with the EFDf at a dose of 3 mg/kg significantly attenuated the paw oedema, polymorphonuclear leukocyte migration, iNOS expression, and tumour necrosis factor alpha (TNF-α) production.
33156477	10	57	from	time	1539:1542	arg1	model					1584:1588	the LPS-induced thermal hyperalgesia model	1547:1588	the LPS-induced thermal hyperalgesia model	1547:1588	The EFDf (10 and 30 mg/kg) also inhibited both phases of the formalin test and caused a significant increase in the reaction time in the LPS-induced thermal hyperalgesia model.
33156477	1	58	theme	furfuracea	257:266	arg1	bark					240:243	the underground stem bark	219:243	the underground stem bark	219:243	A previous study reported the in vivo anti-inflammatory and antinociceptive activities of essential oil of the underground stem bark of Duguetia furfuracea, termed EODf.
33156477	11	59	theme	EFDf-treated	1600:1611	arg1	animals					1613:1619	EFDf-treated animals	1600:1619	EFDf-treated animals	1600:1619	Finally, EFDf-treated animals did not show any alteration of motor coordination.
33156477	1	60	theme	essential	202:210	arg1	oil					212:214	essential oil	202:214	essential oil of the underground stem bark of Duguetia furfuracea, termed EODf	202:279	A previous study reported the in vivo anti-inflammatory and antinociceptive activities of essential oil of the underground stem bark of Duguetia furfuracea, termed EODf.
33156477	1	61	theme	underground	223:233	arg1	bark					240:243	the underground stem bark	219:243	the underground stem bark	219:243	A previous study reported the in vivo anti-inflammatory and antinociceptive activities of essential oil of the underground stem bark of Duguetia furfuracea, termed EODf.
33156477	6	62	dep	in	847:848	arg1	vivo					850:853	vivo	850:853	vivo	850:853	Moreover, the in vivo antinociceptive effect was assayed using the formalin test and the LPS-induced thermal hyperalgesia model.
33156477	5	63	theme	inducible	750:758	arg1	iNOS					783:786	iNOS	783:786	iNOS	783:786	The effects of the EFDf on the polymorphonuclear leukocyte recruitment and the inducible nitric oxide synthase (iNOS) expression were evaluated in mice footpads.
33156477	5	63	theme	inducible	750:758	arg1	synthase					773:780	inducible nitric oxide synthase	750:780	the inducible nitric oxide synthase (iNOS) expression	746:798	The effects of the EFDf on the polymorphonuclear leukocyte recruitment and the inducible nitric oxide synthase (iNOS) expression were evaluated in mice footpads.
33156477	1	64	theme	bark	240:243	arg1	oil					212:214	essential oil	202:214	essential oil of the underground stem bark of Duguetia furfuracea, termed EODf	202:279	A previous study reported the in vivo anti-inflammatory and antinociceptive activities of essential oil of the underground stem bark of Duguetia furfuracea, termed EODf.
33156477	9	65	theme	alpha	1388:1392	arg1	production					1402:1411	the paw oedema, polymorphonuclear leukocyte migration, iNOS expression, and tumour necrosis factor alpha (TNF-α) production	1289:1411	the paw oedema, polymorphonuclear leukocyte migration, iNOS expression, and tumour necrosis factor alpha (TNF-α) production	1289:1411	The oral (p.o.) treatment with the EFDf at a dose of 3 mg/kg significantly attenuated the paw oedema, polymorphonuclear leukocyte migration, iNOS expression, and tumour necrosis factor alpha (TNF-α) production.
33156477	13	66	from	similar	1885:1891	arg1	contrast					1830:1837	contrast	1830:1837	contrast	1830:1837	In contrast, the antinociception promoted by the EFDf was similar to the EODf and was mediated via activation of adenosinergic and opioidergic receptors.
33156477	5	67	theme	leukocyte	720:728	arg1	recruitment					730:740	the polymorphonuclear leukocyte recruitment	698:740	the polymorphonuclear leukocyte recruitment	698:740	The effects of the EFDf on the polymorphonuclear leukocyte recruitment and the inducible nitric oxide synthase (iNOS) expression were evaluated in mice footpads.
33156477	0	68	theme	Duguetia	91:98	arg1	furfuracea					100:109	Duguetia furfuracea	91:109	Duguetia furfuracea	91:109	Anti-inflammatory and antinociceptive activities of a phenylpropanoid-enriched fraction of Duguetia furfuracea.
33156477	12	69	theme	EFDf	1800:1803	arg1	activity					1784:1791	the anti-inflammatory activity	1762:1791	the anti-inflammatory activity of the EFDf	1762:1803	The results suggest that the enrichment of 2,4,5-trimethoxystyrene and α-asarone enhances the anti-inflammatory activity of the EFDf compared to the EODf.
33156477	5	70	theme	oxide	767:771	arg1	iNOS					783:786	iNOS	783:786	iNOS	783:786	The effects of the EFDf on the polymorphonuclear leukocyte recruitment and the inducible nitric oxide synthase (iNOS) expression were evaluated in mice footpads.
33156477	5	70	theme	oxide	767:771	arg1	synthase					773:780	inducible nitric oxide synthase	750:780	the inducible nitric oxide synthase (iNOS) expression	746:798	The effects of the EFDf on the polymorphonuclear leukocyte recruitment and the inducible nitric oxide synthase (iNOS) expression were evaluated in mice footpads.
33156477	8	71	located	found	1150:1154	arg2	concentration					1101:1113	A higher concentration	1092:1113	A higher concentration of phenylpropanoid derivatives	1092:1144	A higher concentration of phenylpropanoid derivatives was found in the EFDf, 64.2% compared to the EODf (38%).
33156477	8	71	located	found	1150:1154	arg1	EFDf					1163:1166	the EFDf	1159:1166	the EFDf	1159:1166	A higher concentration of phenylpropanoid derivatives was found in the EFDf, 64.2% compared to the EODf (38%).
33156477	9	72	with	treatment	1219:1227	arg1	EFDf					1238:1241	the EFDf	1234:1241	the EFDf	1234:1241	The oral (p.o.) treatment with the EFDf at a dose of 3 mg/kg significantly attenuated the paw oedema, polymorphonuclear leukocyte migration, iNOS expression, and tumour necrosis factor alpha (TNF-α) production.
33156477	7	73	theme	major	977:981	arg1	compounds					983:991	8 major compounds	975:991	8 major compounds	975:991	In the EFDf, 8 major compounds were identified, with α-asarone (36.4%) and 2,4,5-trimethoxystyrene (27.8%) the main constituents.
33156477	1	74	dep	in	142:143	arg1	vivo					145:148	vivo	145:148	vivo	145:148	A previous study reported the in vivo anti-inflammatory and antinociceptive activities of essential oil of the underground stem bark of Duguetia furfuracea, termed EODf.
33156477	10	75	theme	hyperalgesia	1571:1582	arg1	model					1584:1588	the LPS-induced thermal hyperalgesia model	1547:1588	the LPS-induced thermal hyperalgesia model	1547:1588	The EFDf (10 and 30 mg/kg) also inhibited both phases of the formalin test and caused a significant increase in the reaction time in the LPS-induced thermal hyperalgesia model.
33156477	2	76	dep	D.	354:355	arg1	furfuracea					357:366	D. furfuracea	354:366	D. furfuracea	354:366	This study aimed to obtain a phenylpropanoid-enriched fraction from the D. furfuracea (EFDf) essential oil and to investigate its anti-inflammatory and antinociceptive effects.
33156477	10	77	theme	LPS-induced	1551:1561	arg1	hyperalgesia					1571:1582	the LPS-induced thermal hyperalgesia	1547:1582	the LPS-induced thermal hyperalgesia model	1547:1588	The EFDf (10 and 30 mg/kg) also inhibited both phases of the formalin test and caused a significant increase in the reaction time in the LPS-induced thermal hyperalgesia model.
33156477	5	78	from	effects	675:681	arg1	expression					789:798	the inducible nitric oxide synthase (iNOS) expression	746:798	the inducible nitric oxide synthase (iNOS) expression	746:798	The effects of the EFDf on the polymorphonuclear leukocyte recruitment and the inducible nitric oxide synthase (iNOS) expression were evaluated in mice footpads.
33156477	5	78	from	effects	675:681	arg1	recruitment					730:740	the polymorphonuclear leukocyte recruitment	698:740	the polymorphonuclear leukocyte recruitment	698:740	The effects of the EFDf on the polymorphonuclear leukocyte recruitment and the inducible nitric oxide synthase (iNOS) expression were evaluated in mice footpads.
33156477	9	79	theme	oedema	1297:1302	arg1	production					1402:1411	the paw oedema, polymorphonuclear leukocyte migration, iNOS expression, and tumour necrosis factor alpha (TNF-α) production	1289:1411	the paw oedema, polymorphonuclear leukocyte migration, iNOS expression, and tumour necrosis factor alpha (TNF-α) production	1289:1411	The oral (p.o.) treatment with the EFDf at a dose of 3 mg/kg significantly attenuated the paw oedema, polymorphonuclear leukocyte migration, iNOS expression, and tumour necrosis factor alpha (TNF-α) production.
33156477	8	80	theme	higher	1094:1099	arg1	concentration					1101:1113	A higher concentration	1092:1113	A higher concentration of phenylpropanoid derivatives	1092:1144	A higher concentration of phenylpropanoid derivatives was found in the EFDf, 64.2% compared to the EODf (38%).
33156477	4	81	theme	in	564:565	arg1	activity					590:597	The in vivo anti-inflammatory activity	560:597	The in vivo anti-inflammatory activity	560:597	The in vivo anti-inflammatory activity was evaluated with a lipopolysaccharide (LPS)-induced paw oedema model.
33156477	13	82	theme	adenosinergic	1940:1952	arg1	receptors					1970:1978	adenosinergic and opioidergic receptors	1940:1978	adenosinergic and opioidergic receptors	1940:1978	In contrast, the antinociception promoted by the EFDf was similar to the EODf and was mediated via activation of adenosinergic and opioidergic receptors.
33156477	3	83	theme	EFDf	491:494	arg1	composition					472:482	The chemical composition	459:482	The chemical composition of the EFDf	459:494	The chemical composition of the EFDf was determined by gas chromatography-mass spectrometry (GC-MS).
33156477	0	84	theme	fraction	79:86	arg1	activities					38:47	Anti-inflammatory and antinociceptive activities	0:47	Anti-inflammatory and antinociceptive activities of a phenylpropanoid-enriched fraction of Duguetia furfuracea.	0:110	Anti-inflammatory and antinociceptive activities of a phenylpropanoid-enriched fraction of Duguetia furfuracea.
33156477	9	85	theme	polymorphonuclear	1305:1321	arg1	migration					1333:1341	polymorphonuclear leukocyte migration	1305:1341	polymorphonuclear leukocyte migration	1305:1341	The oral (p.o.) treatment with the EFDf at a dose of 3 mg/kg significantly attenuated the paw oedema, polymorphonuclear leukocyte migration, iNOS expression, and tumour necrosis factor alpha (TNF-α) production.
33156477	4	86	theme	anti-inflammatory	572:588	arg1	activity					590:597	The in vivo anti-inflammatory activity	560:597	The in vivo anti-inflammatory activity	560:597	The in vivo anti-inflammatory activity was evaluated with a lipopolysaccharide (LPS)-induced paw oedema model.
33156477	13	87	theme	opioidergic	1958:1968	arg1	receptors					1970:1978	adenosinergic and opioidergic receptors	1940:1978	adenosinergic and opioidergic receptors	1940:1978	In contrast, the antinociception promoted by the EFDf was similar to the EODf and was mediated via activation of adenosinergic and opioidergic receptors.
33156477	9	88	theme	migration	1333:1341	arg1	production					1402:1411	the paw oedema, polymorphonuclear leukocyte migration, iNOS expression, and tumour necrosis factor alpha (TNF-α) production	1289:1411	the paw oedema, polymorphonuclear leukocyte migration, iNOS expression, and tumour necrosis factor alpha (TNF-α) production	1289:1411	The oral (p.o.) treatment with the EFDf at a dose of 3 mg/kg significantly attenuated the paw oedema, polymorphonuclear leukocyte migration, iNOS expression, and tumour necrosis factor alpha (TNF-α) production.
33156477	8	89	theme	derivatives	1134:1144	arg1	concentration					1101:1113	A higher concentration	1092:1113	A higher concentration of phenylpropanoid derivatives	1092:1144	A higher concentration of phenylpropanoid derivatives was found in the EFDf, 64.2% compared to the EODf (38%).
33156477	12	90	theme	anti-inflammatory	1766:1782	arg1	activity					1784:1791	the anti-inflammatory activity	1762:1791	the anti-inflammatory activity of the EFDf	1762:1803	The results suggest that the enrichment of 2,4,5-trimethoxystyrene and α-asarone enhances the anti-inflammatory activity of the EFDf compared to the EODf.
33156477	4	91	theme	-induced	644:651	arg1	model					664:668	a lipopolysaccharide (LPS)-induced paw oedema model	618:668	a lipopolysaccharide (LPS)-induced paw oedema model	618:668	The in vivo anti-inflammatory activity was evaluated with a lipopolysaccharide (LPS)-induced paw oedema model.
33156477	3	92	theme	gas	514:516	arg1	GC-MS					552:556	GC-MS	552:556	GC-MS	552:556	The chemical composition of the EFDf was determined by gas chromatography-mass spectrometry (GC-MS).
33156477	3	92	theme	gas	514:516	arg1	spectrometry					538:549	gas chromatography-mass spectrometry	514:549	gas chromatography-mass spectrometry (GC-MS)	514:557	The chemical composition of the EFDf was determined by gas chromatography-mass spectrometry (GC-MS).
33156477	9	93	theme	iNOS	1344:1347	arg1	expression					1349:1358	iNOS expression	1344:1358	iNOS expression	1344:1358	The oral (p.o.) treatment with the EFDf at a dose of 3 mg/kg significantly attenuated the paw oedema, polymorphonuclear leukocyte migration, iNOS expression, and tumour necrosis factor alpha (TNF-α) production.
33156477	4	94	theme	oedema	657:662	arg1	model					664:668	a lipopolysaccharide (LPS)-induced paw oedema model	618:668	a lipopolysaccharide (LPS)-induced paw oedema model	618:668	The in vivo anti-inflammatory activity was evaluated with a lipopolysaccharide (LPS)-induced paw oedema model.
35095858	4	0	theme	mg/kg/day	897:905	arg1	chitosan					907:914	1 mg/kg/day chitosan	895:914	1 mg/kg/day chitosan	895:914	The unchallenged mice received a basal diet (control), and three groups of mice challenged with 1 × 106 C. parvum received a basal diet, a diet supplemented with 50 mg/kg/day paromomycin, and 1 mg/kg/day chitosan, and unchallenged mice treated with 1 mg/kg/day chitosan.
35095858	6	1	theme	infected	1309:1316	arg1	mice					1318:1321	infected mice	1309:1321	infected mice that received no treatment	1309:1348	Chitosan-fed infected mice showed significantly (p < 0.01) decreased mRNA expression levels of interferon-gamma (IFN-γ) and tumor necrosis factor-α (TNF-α) compared to infected mice that received no treatment.
35095858	2	2	theme	chitosan	393:400	arg1	supplementation					402:416	chitosan supplementation	393:416	chitosan supplementation	393:416	This study investigated whether chitosan supplementation modulates the gut microbiota and mediates the TLR4/STAT1 signaling pathways and related cytokines to attenuate C. parvum infection in immunosuppressed mice.
35095858	4	3	theme	basal	679:683	arg1	control					691:697	control	691:697	control	691:697	The unchallenged mice received a basal diet (control), and three groups of mice challenged with 1 × 106 C. parvum received a basal diet, a diet supplemented with 50 mg/kg/day paromomycin, and 1 mg/kg/day chitosan, and unchallenged mice treated with 1 mg/kg/day chitosan.
35095858	4	3	theme	basal	679:683	arg1	diet					685:688	a basal diet	677:688	a basal diet (control)	677:698	The unchallenged mice received a basal diet (control), and three groups of mice challenged with 1 × 106 C. parvum received a basal diet, a diet supplemented with 50 mg/kg/day paromomycin, and 1 mg/kg/day chitosan, and unchallenged mice treated with 1 mg/kg/day chitosan.
35095858	3	4	theme	treatment	628:636	arg1	groups					638:643	five treatment groups	623:643	five treatment groups	623:643	Immunosuppressed C57BL/6 mice were divided into five treatment groups.
35095858	6	5	theme	mRNA	1210:1213	arg1	levels					1226:1231	mRNA expression levels	1210:1231	mRNA expression levels of interferon-gamma (IFN-γ) and tumor necrosis factor-α (TNF-α)	1210:1295	Chitosan-fed infected mice showed significantly (p < 0.01) decreased mRNA expression levels of interferon-gamma (IFN-γ) and tumor necrosis factor-α (TNF-α) compared to infected mice that received no treatment.
35095858	4	6	theme	mice	721:724	arg1	mice					721:724	mice	721:724	mice challenged with 1 × 106 C. parvum	721:758	The unchallenged mice received a basal diet (control), and three groups of mice challenged with 1 × 106 C. parvum received a basal diet, a diet supplemented with 50 mg/kg/day paromomycin, and 1 mg/kg/day chitosan, and unchallenged mice treated with 1 mg/kg/day chitosan.
35095858	4	6	theme	mice	721:724	arg1	groups					711:716	three groups	705:716	three groups of mice challenged with 1 × 106 C. parvum	705:758	The unchallenged mice received a basal diet (control), and three groups of mice challenged with 1 × 106 C. parvum received a basal diet, a diet supplemented with 50 mg/kg/day paromomycin, and 1 mg/kg/day chitosan, and unchallenged mice treated with 1 mg/kg/day chitosan.
35095858	6	7	theme	factor-α	1280:1287	arg1	levels					1226:1231	mRNA expression levels	1210:1231	mRNA expression levels of interferon-gamma (IFN-γ) and tumor necrosis factor-α (TNF-α)	1210:1295	Chitosan-fed infected mice showed significantly (p < 0.01) decreased mRNA expression levels of interferon-gamma (IFN-γ) and tumor necrosis factor-α (TNF-α) compared to infected mice that received no treatment.
35095858	3	8	theme	C57BL/6	592:598	arg1	mice					600:603	Immunosuppressed C57BL/6 mice	575:603	Immunosuppressed C57BL/6 mice	575:603	Immunosuppressed C57BL/6 mice were divided into five treatment groups.
35095858	8	9	theme	16S	1469:1471	arg1	sequencing					1478:1487	16S rRNA sequencing	1469:1487	16S rRNA sequencing analysis	1469:1496	16S rRNA sequencing analysis revealed that chitosan supplementation increased the relative abundance of Bacteroidetes/Bacteroides, while that of Proteobacteria, Tenericutes, Defferribacteres, and Firmicutes decreased (p < 0.05).
35095858	6	10	theme	necrosis	1271:1278	arg1	TNF-α					1290:1294	TNF-α	1290:1294	TNF-α	1290:1294	Chitosan-fed infected mice showed significantly (p < 0.01) decreased mRNA expression levels of interferon-gamma (IFN-γ) and tumor necrosis factor-α (TNF-α) compared to infected mice that received no treatment.
35095858	6	10	theme	necrosis	1271:1278	arg1	factor-α					1280:1287	tumor necrosis factor-α	1265:1287	tumor necrosis factor-α (TNF-α)	1265:1295	Chitosan-fed infected mice showed significantly (p < 0.01) decreased mRNA expression levels of interferon-gamma (IFN-γ) and tumor necrosis factor-α (TNF-α) compared to infected mice that received no treatment.
35095858	1	11	theme	young	244:248	arg1	ruminants					250:258	young ruminants	244:258	young ruminants	244:258	Cryptosporidium parvum infection is very common in infants, immunocompromised patients, or in young ruminants, and chitosan supplementation exhibits beneficial effects against the infection caused by C. parvum.
35095858	9	12	theme	parvum	1777:1782	arg1	infection					1784:1792	C. parvum infection	1774:1792	C. parvum infection	1774:1792	Overall, the findings revealed that chitosan supplementation can ameliorate C. parvum infection by remodeling the composition of the gut microbiota of mice, leading to mediated STAT1/TLR4 up- and downregulation and decreased production of IFN-γ and TNF-α, and these changes resulted in better resolution and control of C. parvum infection.
35095858	8	13	theme	sequencing	1478:1487	arg1	analysis					1489:1496	16S rRNA sequencing analysis	1469:1496	16S rRNA sequencing analysis	1469:1496	16S rRNA sequencing analysis revealed that chitosan supplementation increased the relative abundance of Bacteroidetes/Bacteroides, while that of Proteobacteria, Tenericutes, Defferribacteres, and Firmicutes decreased (p < 0.05).
35095858	7	14	theme	protein	1411:1417	arg1	expression					1419:1428	STAT1 protein expression	1405:1428	STAT1 protein expression (p < 0.01) in C. parvum-infected mice	1405:1466	Chitosan significantly inhibited TLR4 and upregulated STAT1 protein expression (p < 0.01) in C. parvum-infected mice.
35095858	7	14	theme	protein	1411:1417	arg1	<					1433:1433	p < 0.01	1431:1438	p < 0.01	1431:1438	Chitosan significantly inhibited TLR4 and upregulated STAT1 protein expression (p < 0.01) in C. parvum-infected mice.
35095858	9	15	dep	mice	1849:1852	arg1	resulted					1972:1979	resulted	1972:1979	resulted in better resolution and control of C. parvum infection	1972:2035	Overall, the findings revealed that chitosan supplementation can ameliorate C. parvum infection by remodeling the composition of the gut microbiota of mice, leading to mediated STAT1/TLR4 up- and downregulation and decreased production of IFN-γ and TNF-α, and these changes resulted in better resolution and control of C. parvum infection.
35095858	9	15	dep	mice	1849:1852	arg1	leading					1855:1861	leading	1855:1861	leading to mediated STAT1/TLR4 up- and downregulation and decreased production of IFN-γ and TNF-α	1855:1951	Overall, the findings revealed that chitosan supplementation can ameliorate C. parvum infection by remodeling the composition of the gut microbiota of mice, leading to mediated STAT1/TLR4 up- and downregulation and decreased production of IFN-γ and TNF-α, and these changes resulted in better resolution and control of C. parvum infection.
35095858	1	16	theme	Cryptosporidium	150:164	arg1	parvum					166:171	Cryptosporidium parvum	150:171	Cryptosporidium parvum infection	150:181	Cryptosporidium parvum infection is very common in infants, immunocompromised patients, or in young ruminants, and chitosan supplementation exhibits beneficial effects against the infection caused by C. parvum.
35095858	9	17	theme	parvum	2020:2025	arg1	infection					2027:2035	C. parvum infection	2017:2035	C. parvum infection	2017:2035	Overall, the findings revealed that chitosan supplementation can ameliorate C. parvum infection by remodeling the composition of the gut microbiota of mice, leading to mediated STAT1/TLR4 up- and downregulation and decreased production of IFN-γ and TNF-α, and these changes resulted in better resolution and control of C. parvum infection.
35095858	4	18	theme	basal	771:775	arg1	diet					777:780	a basal diet	769:780	a basal diet	769:780	The unchallenged mice received a basal diet (control), and three groups of mice challenged with 1 × 106 C. parvum received a basal diet, a diet supplemented with 50 mg/kg/day paromomycin, and 1 mg/kg/day chitosan, and unchallenged mice treated with 1 mg/kg/day chitosan.
35095858	8	19	theme	chitosan	1512:1519	arg1	supplementation					1521:1535	chitosan supplementation	1512:1535	chitosan supplementation	1512:1535	16S rRNA sequencing analysis revealed that chitosan supplementation increased the relative abundance of Bacteroidetes/Bacteroides, while that of Proteobacteria, Tenericutes, Defferribacteres, and Firmicutes decreased (p < 0.05).
35095858	6	20	theme	interferon-gamma	1236:1251	arg1	levels					1226:1231	mRNA expression levels	1210:1231	mRNA expression levels of interferon-gamma (IFN-γ) and tumor necrosis factor-α (TNF-α)	1210:1295	Chitosan-fed infected mice showed significantly (p < 0.01) decreased mRNA expression levels of interferon-gamma (IFN-γ) and tumor necrosis factor-α (TNF-α) compared to infected mice that received no treatment.
35095858	1	21	from	infants	201:207	arg1	common					191:196	common	191:196	common	191:196	Cryptosporidium parvum infection is very common in infants, immunocompromised patients, or in young ruminants, and chitosan supplementation exhibits beneficial effects against the infection caused by C. parvum.
35095858	2	22	theme	immunosuppressed	552:567	arg1	mice					569:572	immunosuppressed mice	552:572	immunosuppressed mice	552:572	This study investigated whether chitosan supplementation modulates the gut microbiota and mediates the TLR4/STAT1 signaling pathways and related cytokines to attenuate C. parvum infection in immunosuppressed mice.
35095858	0	23	theme	Signaling	100:108	arg1	Pathways					110:117	TLR4/STAT1 Signaling Pathways	89:117	TLR4/STAT1 Signaling Pathways	89:117	Chitosan Protects Immunosuppressed Mice Against Cryptosporidium parvum Infection Through TLR4/STAT1 Signaling Pathways and Gut Microbiota Modulation.
35095858	1	24	from	common	191:196	arg1	infants					201:207	infants	201:207	infants	201:207	Cryptosporidium parvum infection is very common in infants, immunocompromised patients, or in young ruminants, and chitosan supplementation exhibits beneficial effects against the infection caused by C. parvum.
35095858	1	24	from	common	191:196	arg1	patients					228:235	immunocompromised patients	210:235	immunocompromised patients	210:235	Cryptosporidium parvum infection is very common in infants, immunocompromised patients, or in young ruminants, and chitosan supplementation exhibits beneficial effects against the infection caused by C. parvum.
35095858	1	24	from	common	191:196	arg1	ruminants					250:258	young ruminants	244:258	young ruminants	244:258	Cryptosporidium parvum infection is very common in infants, immunocompromised patients, or in young ruminants, and chitosan supplementation exhibits beneficial effects against the infection caused by C. parvum.
35095858	5	25	theme	serum	952:956	arg1	indices					970:976	serum biochemical indices	952:976	serum biochemical indices	952:976	Chitosan supplementation regulated serum biochemical indices and significantly (p < 0.01) reduced C. parvum oocyst excretion in infected mice treated with chitosan compared with the infected mice that received no treatment.
35095858	9	26	theme	gut	1831:1833	arg1	microbiota					1835:1844	the gut microbiota	1827:1844	the gut microbiota of mice, leading to mediated STAT1/TLR4 up- and downregulation and decreased production of IFN-γ and TNF-α, and these changes resulted in better resolution and control of C. parvum infection	1827:2035	Overall, the findings revealed that chitosan supplementation can ameliorate C. parvum infection by remodeling the composition of the gut microbiota of mice, leading to mediated STAT1/TLR4 up- and downregulation and decreased production of IFN-γ and TNF-α, and these changes resulted in better resolution and control of C. parvum infection.
35095858	8	27	theme	relative	1551:1558	arg1	abundance					1560:1568	the relative abundance	1547:1568	the relative abundance of Bacteroidetes/Bacteroides	1547:1597	16S rRNA sequencing analysis revealed that chitosan supplementation increased the relative abundance of Bacteroidetes/Bacteroides, while that of Proteobacteria, Tenericutes, Defferribacteres, and Firmicutes decreased (p < 0.05).
35095858	4	28	theme	mg/kg/day	811:819	arg1	paromomycin					821:831	50 mg/kg/day paromomycin	808:831	50 mg/kg/day paromomycin	808:831	The unchallenged mice received a basal diet (control), and three groups of mice challenged with 1 × 106 C. parvum received a basal diet, a diet supplemented with 50 mg/kg/day paromomycin, and 1 mg/kg/day chitosan, and unchallenged mice treated with 1 mg/kg/day chitosan.
35095858	0	29	theme	Microbiota	127:136	arg1	Modulation					138:147	Gut Microbiota Modulation	123:147	Gut Microbiota Modulation	123:147	Chitosan Protects Immunosuppressed Mice Against Cryptosporidium parvum Infection Through TLR4/STAT1 Signaling Pathways and Gut Microbiota Modulation.
35095858	5	30	theme	parvum	1018:1023	arg1	excretion					1032:1040	C. parvum oocyst excretion	1015:1040	C. parvum oocyst excretion in infected mice treated with chitosan compared with the infected mice that received no treatment	1015:1138	Chitosan supplementation regulated serum biochemical indices and significantly (p < 0.01) reduced C. parvum oocyst excretion in infected mice treated with chitosan compared with the infected mice that received no treatment.
35095858	2	31	theme	related	498:504	arg1	cytokines					506:514	related cytokines	498:514	related cytokines	498:514	This study investigated whether chitosan supplementation modulates the gut microbiota and mediates the TLR4/STAT1 signaling pathways and related cytokines to attenuate C. parvum infection in immunosuppressed mice.
35095858	0	32	theme	Immunosuppressed	18:33	arg1	Mice					35:38	Immunosuppressed Mice	18:38	Immunosuppressed Mice	18:38	Chitosan Protects Immunosuppressed Mice Against Cryptosporidium parvum Infection Through TLR4/STAT1 Signaling Pathways and Gut Microbiota Modulation.
35095858	9	33	theme	STAT1/TLR4	1875:1884	arg1	up-					1886:1888	mediated STAT1/TLR4 up-	1866:1888	mediated STAT1/TLR4 up-	1866:1888	Overall, the findings revealed that chitosan supplementation can ameliorate C. parvum infection by remodeling the composition of the gut microbiota of mice, leading to mediated STAT1/TLR4 up- and downregulation and decreased production of IFN-γ and TNF-α, and these changes resulted in better resolution and control of C. parvum infection.
35095858	2	34	theme	TLR4/STAT1	464:473	arg1	pathways					485:492	the TLR4/STAT1 signaling pathways	460:492	the TLR4/STAT1 signaling pathways	460:492	This study investigated whether chitosan supplementation modulates the gut microbiota and mediates the TLR4/STAT1 signaling pathways and related cytokines to attenuate C. parvum infection in immunosuppressed mice.
35095858	2	35	theme	C.	529:530	arg1	infection					539:547	C. parvum infection	529:547	C. parvum infection in immunosuppressed mice	529:572	This study investigated whether chitosan supplementation modulates the gut microbiota and mediates the TLR4/STAT1 signaling pathways and related cytokines to attenuate C. parvum infection in immunosuppressed mice.
35095858	0	36	theme	parvum	64:69	arg1	Infection					71:79	Cryptosporidium parvum Infection	48:79	Cryptosporidium parvum Infection	48:79	Chitosan Protects Immunosuppressed Mice Against Cryptosporidium parvum Infection Through TLR4/STAT1 Signaling Pathways and Gut Microbiota Modulation.
35095858	6	37	dep	showed	1168:1173	arg1	decreased					1200:1208	decreased	1200:1208	showed significantly (p < 0.01) decreased mRNA expression levels of interferon-gamma (IFN-γ) and tumor necrosis factor-α (TNF-α) compared to infected mice that received no treatment	1168:1348	Chitosan-fed infected mice showed significantly (p < 0.01) decreased mRNA expression levels of interferon-gamma (IFN-γ) and tumor necrosis factor-α (TNF-α) compared to infected mice that received no treatment.
35095858	4	38	theme	1	838:838	arg1	mg/kg/day					840:848	mg/kg/day	840:848	mg/kg/day	840:848	The unchallenged mice received a basal diet (control), and three groups of mice challenged with 1 × 106 C. parvum received a basal diet, a diet supplemented with 50 mg/kg/day paromomycin, and 1 mg/kg/day chitosan, and unchallenged mice treated with 1 mg/kg/day chitosan.
35095858	5	39	theme	infected	1045:1052	arg1	mice					1054:1057	infected mice	1045:1057	infected mice treated with chitosan compared with the infected mice that received no treatment	1045:1138	Chitosan supplementation regulated serum biochemical indices and significantly (p < 0.01) reduced C. parvum oocyst excretion in infected mice treated with chitosan compared with the infected mice that received no treatment.
35095858	6	40	theme	Chitosan-fed	1141:1152	arg1	mice					1163:1166	Chitosan-fed infected mice	1141:1166	Chitosan-fed infected mice	1141:1166	Chitosan-fed infected mice showed significantly (p < 0.01) decreased mRNA expression levels of interferon-gamma (IFN-γ) and tumor necrosis factor-α (TNF-α) compared to infected mice that received no treatment.
35095858	9	41	theme	IFN-γ	1937:1941	arg1	production					1923:1932	decreased production	1913:1932	decreased production of IFN-γ and TNF-α	1913:1951	Overall, the findings revealed that chitosan supplementation can ameliorate C. parvum infection by remodeling the composition of the gut microbiota of mice, leading to mediated STAT1/TLR4 up- and downregulation and decreased production of IFN-γ and TNF-α, and these changes resulted in better resolution and control of C. parvum infection.
35095858	9	41	theme	IFN-γ	1937:1941	arg1	up-					1886:1888	mediated STAT1/TLR4 up-	1866:1888	mediated STAT1/TLR4 up-	1866:1888	Overall, the findings revealed that chitosan supplementation can ameliorate C. parvum infection by remodeling the composition of the gut microbiota of mice, leading to mediated STAT1/TLR4 up- and downregulation and decreased production of IFN-γ and TNF-α, and these changes resulted in better resolution and control of C. parvum infection.
35095858	9	41	theme	IFN-γ	1937:1941	arg1	downregulation					1894:1907	downregulation	1894:1907	downregulation	1894:1907	Overall, the findings revealed that chitosan supplementation can ameliorate C. parvum infection by remodeling the composition of the gut microbiota of mice, leading to mediated STAT1/TLR4 up- and downregulation and decreased production of IFN-γ and TNF-α, and these changes resulted in better resolution and control of C. parvum infection.
35095858	3	42	theme	Immunosuppressed	575:590	arg1	mice					600:603	Immunosuppressed C57BL/6 mice	575:603	Immunosuppressed C57BL/6 mice	575:603	Immunosuppressed C57BL/6 mice were divided into five treatment groups.
35095858	4	43	theme	1	895:895	arg1	mg/kg/day					897:905	mg/kg/day	897:905	mg/kg/day	897:905	The unchallenged mice received a basal diet (control), and three groups of mice challenged with 1 × 106 C. parvum received a basal diet, a diet supplemented with 50 mg/kg/day paromomycin, and 1 mg/kg/day chitosan, and unchallenged mice treated with 1 mg/kg/day chitosan.
35095858	9	44	theme	TNF-α	1947:1951	arg1	production					1923:1932	decreased production	1913:1932	decreased production of IFN-γ and TNF-α	1913:1951	Overall, the findings revealed that chitosan supplementation can ameliorate C. parvum infection by remodeling the composition of the gut microbiota of mice, leading to mediated STAT1/TLR4 up- and downregulation and decreased production of IFN-γ and TNF-α, and these changes resulted in better resolution and control of C. parvum infection.
35095858	9	44	theme	TNF-α	1947:1951	arg1	up-					1886:1888	mediated STAT1/TLR4 up-	1866:1888	mediated STAT1/TLR4 up-	1866:1888	Overall, the findings revealed that chitosan supplementation can ameliorate C. parvum infection by remodeling the composition of the gut microbiota of mice, leading to mediated STAT1/TLR4 up- and downregulation and decreased production of IFN-γ and TNF-α, and these changes resulted in better resolution and control of C. parvum infection.
35095858	9	44	theme	TNF-α	1947:1951	arg1	downregulation					1894:1907	downregulation	1894:1907	downregulation	1894:1907	Overall, the findings revealed that chitosan supplementation can ameliorate C. parvum infection by remodeling the composition of the gut microbiota of mice, leading to mediated STAT1/TLR4 up- and downregulation and decreased production of IFN-γ and TNF-α, and these changes resulted in better resolution and control of C. parvum infection.
35095858	8	45	theme	p	1687:1687	arg1	<					1689:1689	p < 0.05	1687:1694	p < 0.05	1687:1694	16S rRNA sequencing analysis revealed that chitosan supplementation increased the relative abundance of Bacteroidetes/Bacteroides, while that of Proteobacteria, Tenericutes, Defferribacteres, and Firmicutes decreased (p < 0.05).
35095858	1	46	theme	beneficial	299:308	arg1	effects					310:316	beneficial effects	299:316	beneficial effects	299:316	Cryptosporidium parvum infection is very common in infants, immunocompromised patients, or in young ruminants, and chitosan supplementation exhibits beneficial effects against the infection caused by C. parvum.
35095858	6	47	theme	expression	1215:1224	arg1	levels					1226:1231	mRNA expression levels	1210:1231	mRNA expression levels of interferon-gamma (IFN-γ) and tumor necrosis factor-α (TNF-α)	1210:1295	Chitosan-fed infected mice showed significantly (p < 0.01) decreased mRNA expression levels of interferon-gamma (IFN-γ) and tumor necrosis factor-α (TNF-α) compared to infected mice that received no treatment.
35095858	4	48	theme	unchallenged	864:875	arg1	mice					877:880	unchallenged mice	864:880	unchallenged mice treated with 1 mg/kg/day chitosan	864:914	The unchallenged mice received a basal diet (control), and three groups of mice challenged with 1 × 106 C. parvum received a basal diet, a diet supplemented with 50 mg/kg/day paromomycin, and 1 mg/kg/day chitosan, and unchallenged mice treated with 1 mg/kg/day chitosan.
35095858	7	49	theme	parvum-infected	1447:1461	arg1	mice					1463:1466	C. parvum-infected mice	1444:1466	C. parvum-infected mice	1444:1466	Chitosan significantly inhibited TLR4 and upregulated STAT1 protein expression (p < 0.01) in C. parvum-infected mice.
35095858	5	50	from	excretion	1032:1040	arg1	mice					1054:1057	infected mice	1045:1057	infected mice treated with chitosan compared with the infected mice that received no treatment	1045:1138	Chitosan supplementation regulated serum biochemical indices and significantly (p < 0.01) reduced C. parvum oocyst excretion in infected mice treated with chitosan compared with the infected mice that received no treatment.
35095858	1	51	theme	immunocompromised	210:226	arg1	infants					201:207	infants	201:207	infants	201:207	Cryptosporidium parvum infection is very common in infants, immunocompromised patients, or in young ruminants, and chitosan supplementation exhibits beneficial effects against the infection caused by C. parvum.
35095858	1	51	theme	immunocompromised	210:226	arg1	patients					228:235	immunocompromised patients	210:235	immunocompromised patients	210:235	Cryptosporidium parvum infection is very common in infants, immunocompromised patients, or in young ruminants, and chitosan supplementation exhibits beneficial effects against the infection caused by C. parvum.
35095858	9	52	theme	chitosan	1734:1741	arg1	supplementation					1743:1757	chitosan supplementation	1734:1757	chitosan supplementation	1734:1757	Overall, the findings revealed that chitosan supplementation can ameliorate C. parvum infection by remodeling the composition of the gut microbiota of mice, leading to mediated STAT1/TLR4 up- and downregulation and decreased production of IFN-γ and TNF-α, and these changes resulted in better resolution and control of C. parvum infection.
35095858	8	53	theme	rRNA	1473:1476	arg1	sequencing					1478:1487	16S rRNA sequencing	1469:1487	16S rRNA sequencing analysis	1469:1496	16S rRNA sequencing analysis revealed that chitosan supplementation increased the relative abundance of Bacteroidetes/Bacteroides, while that of Proteobacteria, Tenericutes, Defferribacteres, and Firmicutes decreased (p < 0.05).
35095858	9	54	theme	C.	1774:1775	arg1	infection					1784:1792	C. parvum infection	1774:1792	C. parvum infection	1774:1792	Overall, the findings revealed that chitosan supplementation can ameliorate C. parvum infection by remodeling the composition of the gut microbiota of mice, leading to mediated STAT1/TLR4 up- and downregulation and decreased production of IFN-γ and TNF-α, and these changes resulted in better resolution and control of C. parvum infection.
35095858	7	55	theme	p	1431:1431	arg1	expression					1419:1428	STAT1 protein expression	1405:1428	STAT1 protein expression (p < 0.01) in C. parvum-infected mice	1405:1466	Chitosan significantly inhibited TLR4 and upregulated STAT1 protein expression (p < 0.01) in C. parvum-infected mice.
35095858	7	55	theme	p	1431:1431	arg1	<					1433:1433	p < 0.01	1431:1438	p < 0.01	1431:1438	Chitosan significantly inhibited TLR4 and upregulated STAT1 protein expression (p < 0.01) in C. parvum-infected mice.
35095858	5	56	theme	biochemical	958:968	arg1	indices					970:976	serum biochemical indices	952:976	serum biochemical indices	952:976	Chitosan supplementation regulated serum biochemical indices and significantly (p < 0.01) reduced C. parvum oocyst excretion in infected mice treated with chitosan compared with the infected mice that received no treatment.
35095858	9	57	theme	C.	2017:2018	arg1	infection					2027:2035	C. parvum infection	2017:2035	C. parvum infection	2017:2035	Overall, the findings revealed that chitosan supplementation can ameliorate C. parvum infection by remodeling the composition of the gut microbiota of mice, leading to mediated STAT1/TLR4 up- and downregulation and decreased production of IFN-γ and TNF-α, and these changes resulted in better resolution and control of C. parvum infection.
35095858	1	58	from	ruminants	250:258	arg1	common					191:196	common	191:196	common	191:196	Cryptosporidium parvum infection is very common in infants, immunocompromised patients, or in young ruminants, and chitosan supplementation exhibits beneficial effects against the infection caused by C. parvum.
35095858	1	59	theme	parvum	166:171	arg1	infection					173:181	Cryptosporidium parvum infection	150:181	Cryptosporidium parvum infection	150:181	Cryptosporidium parvum infection is very common in infants, immunocompromised patients, or in young ruminants, and chitosan supplementation exhibits beneficial effects against the infection caused by C. parvum.
35095858	7	60	theme	STAT1	1405:1409	arg1	expression					1419:1428	STAT1 protein expression	1405:1428	STAT1 protein expression (p < 0.01) in C. parvum-infected mice	1405:1466	Chitosan significantly inhibited TLR4 and upregulated STAT1 protein expression (p < 0.01) in C. parvum-infected mice.
35095858	7	60	theme	STAT1	1405:1409	arg1	<					1433:1433	p < 0.01	1431:1438	p < 0.01	1431:1438	Chitosan significantly inhibited TLR4 and upregulated STAT1 protein expression (p < 0.01) in C. parvum-infected mice.
35095858	0	61	theme	TLR4/STAT1	89:98	arg1	Pathways					110:117	TLR4/STAT1 Signaling Pathways	89:117	TLR4/STAT1 Signaling Pathways	89:117	Chitosan Protects Immunosuppressed Mice Against Cryptosporidium parvum Infection Through TLR4/STAT1 Signaling Pathways and Gut Microbiota Modulation.
35095858	5	62	theme	infected	1099:1106	arg1	mice					1108:1111	the infected mice	1095:1111	the infected mice that received no treatment	1095:1138	Chitosan supplementation regulated serum biochemical indices and significantly (p < 0.01) reduced C. parvum oocyst excretion in infected mice treated with chitosan compared with the infected mice that received no treatment.
35095858	5	63	theme	Chitosan	917:924	arg1	supplementation					926:940	Chitosan supplementation	917:940	Chitosan supplementation	917:940	Chitosan supplementation regulated serum biochemical indices and significantly (p < 0.01) reduced C. parvum oocyst excretion in infected mice treated with chitosan compared with the infected mice that received no treatment.
35095858	9	64	dep	better	1984:1989	arg1	resolution					1991:2000	resolution	1991:2000	resolution	1991:2000	Overall, the findings revealed that chitosan supplementation can ameliorate C. parvum infection by remodeling the composition of the gut microbiota of mice, leading to mediated STAT1/TLR4 up- and downregulation and decreased production of IFN-γ and TNF-α, and these changes resulted in better resolution and control of C. parvum infection.
35095858	0	65	theme	Gut	123:125	arg1	Modulation					138:147	Gut Microbiota Modulation	123:147	Gut Microbiota Modulation	123:147	Chitosan Protects Immunosuppressed Mice Against Cryptosporidium parvum Infection Through TLR4/STAT1 Signaling Pathways and Gut Microbiota Modulation.
35095858	9	66	theme	microbiota	1835:1844	arg1	composition					1812:1822	the composition	1808:1822	the composition of the gut microbiota of mice, leading to mediated STAT1/TLR4 up- and downregulation and decreased production of IFN-γ and TNF-α, and these changes resulted in better resolution and control of C. parvum infection	1808:2035	Overall, the findings revealed that chitosan supplementation can ameliorate C. parvum infection by remodeling the composition of the gut microbiota of mice, leading to mediated STAT1/TLR4 up- and downregulation and decreased production of IFN-γ and TNF-α, and these changes resulted in better resolution and control of C. parvum infection.
35095858	8	67	theme	Bacteroidetes/Bacteroides	1573:1597	arg1	abundance					1560:1568	the relative abundance	1547:1568	the relative abundance of Bacteroidetes/Bacteroides	1547:1597	16S rRNA sequencing analysis revealed that chitosan supplementation increased the relative abundance of Bacteroidetes/Bacteroides, while that of Proteobacteria, Tenericutes, Defferribacteres, and Firmicutes decreased (p < 0.05).
35095858	5	68	dep	significantly	982:994	arg1	p					997:997	p < 0.01	997:1004	p < 0.01	997:1004	Chitosan supplementation regulated serum biochemical indices and significantly (p < 0.01) reduced C. parvum oocyst excretion in infected mice treated with chitosan compared with the infected mice that received no treatment.
35095858	8	69	dep	decreased	1676:1684	arg1	<					1689:1689	p < 0.05	1687:1694	p < 0.05	1687:1694	16S rRNA sequencing analysis revealed that chitosan supplementation increased the relative abundance of Bacteroidetes/Bacteroides, while that of Proteobacteria, Tenericutes, Defferribacteres, and Firmicutes decreased (p < 0.05).
35095858	4	70	dep	×	744:744	arg1	parvum					753:758	C. parvum	750:758	1 × 106 C. parvum	742:758	The unchallenged mice received a basal diet (control), and three groups of mice challenged with 1 × 106 C. parvum received a basal diet, a diet supplemented with 50 mg/kg/day paromomycin, and 1 mg/kg/day chitosan, and unchallenged mice treated with 1 mg/kg/day chitosan.
35095858	9	71	theme	mice	1849:1852	arg1	microbiota					1835:1844	the gut microbiota	1827:1844	the gut microbiota of mice, leading to mediated STAT1/TLR4 up- and downregulation and decreased production of IFN-γ and TNF-α, and these changes resulted in better resolution and control of C. parvum infection	1827:2035	Overall, the findings revealed that chitosan supplementation can ameliorate C. parvum infection by remodeling the composition of the gut microbiota of mice, leading to mediated STAT1/TLR4 up- and downregulation and decreased production of IFN-γ and TNF-α, and these changes resulted in better resolution and control of C. parvum infection.
35095858	2	72	from	infection	539:547	arg1	mice					569:572	immunosuppressed mice	552:572	immunosuppressed mice	552:572	This study investigated whether chitosan supplementation modulates the gut microbiota and mediates the TLR4/STAT1 signaling pathways and related cytokines to attenuate C. parvum infection in immunosuppressed mice.
35095858	4	73	theme	50	808:809	arg1	mg/kg/day					811:819	mg/kg/day	811:819	mg/kg/day	811:819	The unchallenged mice received a basal diet (control), and three groups of mice challenged with 1 × 106 C. parvum received a basal diet, a diet supplemented with 50 mg/kg/day paromomycin, and 1 mg/kg/day chitosan, and unchallenged mice treated with 1 mg/kg/day chitosan.
35095858	2	74	theme	gut	432:434	arg1	microbiota					436:445	the gut microbiota	428:445	the gut microbiota	428:445	This study investigated whether chitosan supplementation modulates the gut microbiota and mediates the TLR4/STAT1 signaling pathways and related cytokines to attenuate C. parvum infection in immunosuppressed mice.
35095858	5	75	theme	<	999:999	arg1	p					997:997	p < 0.01	997:1004	p < 0.01	997:1004	Chitosan supplementation regulated serum biochemical indices and significantly (p < 0.01) reduced C. parvum oocyst excretion in infected mice treated with chitosan compared with the infected mice that received no treatment.
35095858	7	76	from	expression	1419:1428	arg1	mice					1463:1466	C. parvum-infected mice	1444:1466	C. parvum-infected mice	1444:1466	Chitosan significantly inhibited TLR4 and upregulated STAT1 protein expression (p < 0.01) in C. parvum-infected mice.
35095858	9	77	theme	mediated	1866:1873	arg1	up-					1886:1888	mediated STAT1/TLR4 up-	1866:1888	mediated STAT1/TLR4 up-	1866:1888	Overall, the findings revealed that chitosan supplementation can ameliorate C. parvum infection by remodeling the composition of the gut microbiota of mice, leading to mediated STAT1/TLR4 up- and downregulation and decreased production of IFN-γ and TNF-α, and these changes resulted in better resolution and control of C. parvum infection.
35095858	4	78	theme	unchallenged	650:661	arg1	mice					663:666	The unchallenged mice	646:666	The unchallenged mice	646:666	The unchallenged mice received a basal diet (control), and three groups of mice challenged with 1 × 106 C. parvum received a basal diet, a diet supplemented with 50 mg/kg/day paromomycin, and 1 mg/kg/day chitosan, and unchallenged mice treated with 1 mg/kg/day chitosan.
35095858	5	79	theme	C.	1015:1016	arg1	excretion					1032:1040	C. parvum oocyst excretion	1015:1040	C. parvum oocyst excretion in infected mice treated with chitosan compared with the infected mice that received no treatment	1015:1138	Chitosan supplementation regulated serum biochemical indices and significantly (p < 0.01) reduced C. parvum oocyst excretion in infected mice treated with chitosan compared with the infected mice that received no treatment.
35095858	2	80	theme	signaling	475:483	arg1	pathways					485:492	the TLR4/STAT1 signaling pathways	460:492	the TLR4/STAT1 signaling pathways	460:492	This study investigated whether chitosan supplementation modulates the gut microbiota and mediates the TLR4/STAT1 signaling pathways and related cytokines to attenuate C. parvum infection in immunosuppressed mice.
35095858	0	81	theme	Cryptosporidium	48:62	arg1	Infection					71:79	Cryptosporidium parvum Infection	48:79	Cryptosporidium parvum Infection	48:79	Chitosan Protects Immunosuppressed Mice Against Cryptosporidium parvum Infection Through TLR4/STAT1 Signaling Pathways and Gut Microbiota Modulation.
35095858	2	82	theme	parvum	532:537	arg1	infection					539:547	C. parvum infection	529:547	C. parvum infection in immunosuppressed mice	529:572	This study investigated whether chitosan supplementation modulates the gut microbiota and mediates the TLR4/STAT1 signaling pathways and related cytokines to attenuate C. parvum infection in immunosuppressed mice.
35095858	4	83	theme	mg/kg/day	840:848	arg1	chitosan					850:857	1 mg/kg/day chitosan	838:857	1 mg/kg/day chitosan	838:857	The unchallenged mice received a basal diet (control), and three groups of mice challenged with 1 × 106 C. parvum received a basal diet, a diet supplemented with 50 mg/kg/day paromomycin, and 1 mg/kg/day chitosan, and unchallenged mice treated with 1 mg/kg/day chitosan.
35095858	5	84	theme	oocyst	1025:1030	arg1	excretion					1032:1040	C. parvum oocyst excretion	1015:1040	C. parvum oocyst excretion in infected mice treated with chitosan compared with the infected mice that received no treatment	1015:1138	Chitosan supplementation regulated serum biochemical indices and significantly (p < 0.01) reduced C. parvum oocyst excretion in infected mice treated with chitosan compared with the infected mice that received no treatment.
35095858	6	85	theme	tumor	1265:1269	arg1	TNF-α					1290:1294	TNF-α	1290:1294	TNF-α	1290:1294	Chitosan-fed infected mice showed significantly (p < 0.01) decreased mRNA expression levels of interferon-gamma (IFN-γ) and tumor necrosis factor-α (TNF-α) compared to infected mice that received no treatment.
35095858	6	85	theme	tumor	1265:1269	arg1	factor-α					1280:1287	tumor necrosis factor-α	1265:1287	tumor necrosis factor-α (TNF-α)	1265:1295	Chitosan-fed infected mice showed significantly (p < 0.01) decreased mRNA expression levels of interferon-gamma (IFN-γ) and tumor necrosis factor-α (TNF-α) compared to infected mice that received no treatment.
35095858	9	86	theme	decreased	1913:1921	arg1	production					1923:1932	decreased production	1913:1932	decreased production of IFN-γ and TNF-α	1913:1951	Overall, the findings revealed that chitosan supplementation can ameliorate C. parvum infection by remodeling the composition of the gut microbiota of mice, leading to mediated STAT1/TLR4 up- and downregulation and decreased production of IFN-γ and TNF-α, and these changes resulted in better resolution and control of C. parvum infection.
35095858	6	87	theme	infected	1154:1161	arg1	mice					1163:1166	Chitosan-fed infected mice	1141:1166	Chitosan-fed infected mice	1141:1166	Chitosan-fed infected mice showed significantly (p < 0.01) decreased mRNA expression levels of interferon-gamma (IFN-γ) and tumor necrosis factor-α (TNF-α) compared to infected mice that received no treatment.
35095858	6	88	dep	significantly	1175:1187	arg1	p					1190:1190	p	1190:1190	p < 0.01	1190:1197	Chitosan-fed infected mice showed significantly (p < 0.01) decreased mRNA expression levels of interferon-gamma (IFN-γ) and tumor necrosis factor-α (TNF-α) compared to infected mice that received no treatment.
35095858	1	89	theme	chitosan	265:272	arg1	supplementation					274:288	chitosan supplementation	265:288	chitosan supplementation	265:288	Cryptosporidium parvum infection is very common in infants, immunocompromised patients, or in young ruminants, and chitosan supplementation exhibits beneficial effects against the infection caused by C. parvum.
33918094	8	0	theme	-D	1013:1014	arg1	mRNA					1016:1019	SP-A, -C and -D mRNA	1000:1019	SP-A, -C and -D mRNA	1000:1019	There was a reduction in SP-A, -C and -D mRNA, SP-D protein in tissue and SP-A, -C and -D protein in BALF of MPS IIIA mice.
33918094	9	1	from	hysteresis	1264:1273	arg1	surfactant					1287:1296	MPS IIIA surfactant	1278:1296	MPS IIIA surfactant	1278:1296	Captive bubble surfactometry showed an increase in minimum and maximum surface tension and percent surface area compression, as well as a higher compressibility and hysteresis in MPS IIIA surfactant upon dynamic cycling.
33918094	9	1	from	hysteresis	1264:1273	arg1	tension					1178:1184	minimum and maximum surface tension	1150:1184	tension	1178:1184	Captive bubble surfactometry showed an increase in minimum and maximum surface tension and percent surface area compression, as well as a higher compressibility and hysteresis in MPS IIIA surfactant upon dynamic cycling.
33918094	9	1	from	hysteresis	1264:1273	arg1	area					1206:1209	percent surface area	1190:1209	percent surface area	1190:1209	Captive bubble surfactometry showed an increase in minimum and maximum surface tension and percent surface area compression, as well as a higher compressibility and hysteresis in MPS IIIA surfactant upon dynamic cycling.
33918094	6	2	from	esters	836:841	arg1	BALF					860:863	MPS IIIA BALF	851:863	MPS IIIA BALF	851:863	There was an increase in HS and a decrease in BMP and cholesteryl esters (CE) in MPS IIIA BALF.
33918094	10	3	from	function	1435:1442	arg1	IIIA					1451:1454	MPS IIIA	1447:1454	MPS IIIA	1447:1454	Collectively these biochemical and biophysical changes in alveolar surfactant are likely to be detrimental to lung function in MPS IIIA.
33918094	7	4	dep	reduced	945:951	arg1	%					959:959	49.70%	954:959	49.70%	954:959	Phospholipid composition remained unchanged, but BALF total phospholipids were reduced (49.70%) in MPS IIIA.
33918094	4	5	from	lipids	487:492	arg1	tissue					536:541	pulmonary tissue	526:541	pulmonary tissue	526:541	We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age).
33918094	4	5	from	lipids	487:492	arg1	mice					632:635	healthy and diseased mice	611:635	healthy and diseased mice (20 weeks of age)	611:653	We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age).
33918094	4	5	from	lipids	487:492	arg1	BALF					577:580	BALF	577:580	BALF	577:580	We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age).
33918094	4	5	from	lipids	487:492	arg1	fluid					570:574	bronchoalveolar lavage fluid	547:574	bronchoalveolar lavage fluid (BALF)	547:581	We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age).
33918094	0	6	theme	Increased	0:8	arg1	Sulphate					27:34	Increased Alveolar Heparan Sulphate	0:34	Increased Alveolar Heparan Sulphate	0:34	Increased Alveolar Heparan Sulphate and Reduced Pulmonary Surfactant Amount and Function in the Mucopolysaccharidosis IIIA Mouse.
33918094	1	7	theme	lysosomal	173:181	arg1	IIIA					152:155	Mucopolysaccharidosis IIIA	130:155	Mucopolysaccharidosis IIIA (MPS IIIA)	130:166	Mucopolysaccharidosis IIIA (MPS IIIA) is a lysosomal storage disease with significant neurological and skeletal pathologies.
33918094	1	7	theme	lysosomal	173:181	arg1	disease					191:197	a lysosomal storage disease	171:197	a lysosomal storage disease with significant neurological and skeletal pathologies	171:252	Mucopolysaccharidosis IIIA (MPS IIIA) is a lysosomal storage disease with significant neurological and skeletal pathologies.
33918094	6	8	from	increase	783:790	arg1	esters					836:841	cholesteryl esters	824:841	cholesteryl esters	824:841	There was an increase in HS and a decrease in BMP and cholesteryl esters (CE) in MPS IIIA BALF.
33918094	6	8	from	increase	783:790	arg1	CE					844:845	CE	844:845	CE	844:845	There was an increase in HS and a decrease in BMP and cholesteryl esters (CE) in MPS IIIA BALF.
33918094	6	8	from	increase	783:790	arg1	BMP					816:818	BMP	816:818	BMP	816:818	There was an increase in HS and a decrease in BMP and cholesteryl esters (CE) in MPS IIIA BALF.
33918094	6	8	from	increase	783:790	arg1	HS					795:796	HS	795:796	HS	795:796	There was an increase in HS and a decrease in BMP and cholesteryl esters (CE) in MPS IIIA BALF.
33918094	2	9	theme	IIIA	337:340	arg1	patients					342:349	MPS IIIA patients	333:349	MPS IIIA patients	333:349	Respiratory dysfunction is a secondary pathology contributing to mortality in MPS IIIA patients.
33918094	9	10	theme	MPS	1278:1280	arg1	surfactant					1287:1296	MPS IIIA surfactant	1278:1296	MPS IIIA surfactant	1278:1296	Captive bubble surfactometry showed an increase in minimum and maximum surface tension and percent surface area compression, as well as a higher compressibility and hysteresis in MPS IIIA surfactant upon dynamic cycling.
33918094	8	11	theme	SP-A	1049:1052	arg1	protein					1065:1071	SP-A, -C and -D protein	1049:1071	SP-A, -C and -D protein	1049:1071	There was a reduction in SP-A, -C and -D mRNA, SP-D protein in tissue and SP-A, -C and -D protein in BALF of MPS IIIA mice.
33918094	8	12	theme	IIIA	1088:1091	arg1	mice					1093:1096	MPS IIIA mice	1084:1096	MPS IIIA mice	1084:1096	There was a reduction in SP-A, -C and -D mRNA, SP-D protein in tissue and SP-A, -C and -D protein in BALF of MPS IIIA mice.
33918094	7	13	theme	MPS	965:967	arg1	IIIA					969:972	MPS IIIA	965:972	MPS IIIA	965:972	Phospholipid composition remained unchanged, but BALF total phospholipids were reduced (49.70%) in MPS IIIA.
33918094	1	14	theme	significant	204:214	arg1	pathologies					242:252	significant neurological and skeletal pathologies	204:252	significant neurological and skeletal pathologies	204:252	Mucopolysaccharidosis IIIA (MPS IIIA) is a lysosomal storage disease with significant neurological and skeletal pathologies.
33918094	8	15	theme	-C	1055:1056	arg1	protein					1065:1071	SP-A, -C and -D protein	1049:1071	SP-A, -C and -D protein	1049:1071	There was a reduction in SP-A, -C and -D mRNA, SP-D protein in tissue and SP-A, -C and -D protein in BALF of MPS IIIA mice.
33918094	6	16	theme	IIIA	855:858	arg1	BALF					860:863	MPS IIIA BALF	851:863	MPS IIIA BALF	851:863	There was an increase in HS and a decrease in BMP and cholesteryl esters (CE) in MPS IIIA BALF.
33918094	4	17	theme	heparan	464:470	arg1	sulphate					472:479	heparan sulphate	464:479	heparan sulphate (HS)	464:484	We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age).
33918094	4	17	theme	heparan	464:470	arg1	HS					482:483	HS	482:483	HS	482:483	We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age).
33918094	7	18	theme	total	920:924	arg1	phospholipids					926:938	BALF total phospholipids	915:938	BALF total phospholipids	915:938	Phospholipid composition remained unchanged, but BALF total phospholipids were reduced (49.70%) in MPS IIIA.
33918094	9	19	theme	dynamic	1303:1309	arg1	cycling					1311:1317	dynamic cycling	1303:1317	dynamic cycling	1303:1317	Captive bubble surfactometry showed an increase in minimum and maximum surface tension and percent surface area compression, as well as a higher compressibility and hysteresis in MPS IIIA surfactant upon dynamic cycling.
33918094	8	20	theme	-D	1062:1063	arg1	protein					1065:1071	SP-A, -C and -D protein	1049:1071	SP-A, -C and -D protein	1049:1071	There was a reduction in SP-A, -C and -D mRNA, SP-D protein in tissue and SP-A, -C and -D protein in BALF of MPS IIIA mice.
33918094	10	21	theme	MPS	1447:1449	arg1	IIIA					1451:1454	MPS IIIA	1447:1454	MPS IIIA	1447:1454	Collectively these biochemical and biophysical changes in alveolar surfactant are likely to be detrimental to lung function in MPS IIIA.
33918094	1	22	theme	Mucopolysaccharidosis	130:150	arg1	IIIA					152:155	Mucopolysaccharidosis IIIA	130:155	Mucopolysaccharidosis IIIA (MPS IIIA)	130:166	Mucopolysaccharidosis IIIA (MPS IIIA) is a lysosomal storage disease with significant neurological and skeletal pathologies.
33918094	1	22	theme	Mucopolysaccharidosis	130:150	arg1	IIIA					162:165	MPS IIIA	158:165	MPS IIIA	158:165	Mucopolysaccharidosis IIIA (MPS IIIA) is a lysosomal storage disease with significant neurological and skeletal pathologies.
33918094	1	22	theme	Mucopolysaccharidosis	130:150	arg1	disease					191:197	a lysosomal storage disease	171:197	a lysosomal storage disease with significant neurological and skeletal pathologies	171:252	Mucopolysaccharidosis IIIA (MPS IIIA) is a lysosomal storage disease with significant neurological and skeletal pathologies.
33918094	5	23	theme	Heparan	656:662	arg1	sulphate					664:671	Heparan sulphate	656:671	Heparan sulphate	656:671	Heparan sulphate, ganglioside GM3 and bis(monoacylglycero)phosphate (BMP) were increased in MPS IIIA lung tissue.
33918094	2	24	from	mortality	320:328	arg1	patients					342:349	MPS IIIA patients	333:349	MPS IIIA patients	333:349	Respiratory dysfunction is a secondary pathology contributing to mortality in MPS IIIA patients.
33918094	7	25	theme	BALF	915:918	arg1	phospholipids					926:938	BALF total phospholipids	915:938	BALF total phospholipids	915:938	Phospholipid composition remained unchanged, but BALF total phospholipids were reduced (49.70%) in MPS IIIA.
33918094	4	26	from	activity	599:606	arg1	tissue					536:541	pulmonary tissue	526:541	pulmonary tissue	526:541	We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age).
33918094	4	26	from	activity	599:606	arg1	mice					632:635	healthy and diseased mice	611:635	healthy and diseased mice (20 weeks of age)	611:653	We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age).
33918094	4	26	from	activity	599:606	arg1	BALF					577:580	BALF	577:580	BALF	577:580	We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age).
33918094	4	26	from	activity	599:606	arg1	fluid					570:574	bronchoalveolar lavage fluid	547:574	bronchoalveolar lavage fluid (BALF)	547:581	We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age).
33918094	9	27	theme	Captive	1099:1105	arg1	surfactometry					1114:1126	Captive bubble surfactometry	1099:1126	Captive bubble surfactometry	1099:1126	Captive bubble surfactometry showed an increase in minimum and maximum surface tension and percent surface area compression, as well as a higher compressibility and hysteresis in MPS IIIA surfactant upon dynamic cycling.
33918094	5	28	dep	sulphate	664:671	arg1	phosphate					714:722	phosphate	714:722	phosphate (BMP)	714:728	Heparan sulphate, ganglioside GM3 and bis(monoacylglycero)phosphate (BMP) were increased in MPS IIIA lung tissue.
33918094	5	28	dep	sulphate	664:671	arg1	BMP					725:727	BMP	725:727	BMP	725:727	Heparan sulphate, ganglioside GM3 and bis(monoacylglycero)phosphate (BMP) were increased in MPS IIIA lung tissue.
33918094	4	29	theme	surfactant	498:507	arg1	proteins					509:516	surfactant proteins	498:516	surfactant proteins (SP)	498:521	We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age).
33918094	4	29	theme	surfactant	498:507	arg1	SP					519:520	SP	519:520	SP	519:520	We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age).
33918094	3	30	theme	optimal	387:393	arg1	function					400:407	optimal lung function	387:407	optimal lung function	387:407	Pulmonary surfactant is crucial to optimal lung function and has not been investigated in MPS IIIA.
33918094	0	31	theme	IIIA	118:121	arg1	Mouse					123:127	the Mucopolysaccharidosis IIIA Mouse	92:127	the Mucopolysaccharidosis IIIA Mouse	92:127	Increased Alveolar Heparan Sulphate and Reduced Pulmonary Surfactant Amount and Function in the Mucopolysaccharidosis IIIA Mouse.
33918094	5	32	theme	IIIA	752:755	arg1	tissue					762:767	MPS IIIA lung tissue	748:767	MPS IIIA lung tissue	748:767	Heparan sulphate, ganglioside GM3 and bis(monoacylglycero)phosphate (BMP) were increased in MPS IIIA lung tissue.
33918094	0	33	theme	Heparan	19:25	arg1	Sulphate					27:34	Increased Alveolar Heparan Sulphate	0:34	Increased Alveolar Heparan Sulphate	0:34	Increased Alveolar Heparan Sulphate and Reduced Pulmonary Surfactant Amount and Function in the Mucopolysaccharidosis IIIA Mouse.
33918094	9	34	theme	surface	1170:1176	arg1	tension					1178:1184	minimum and maximum surface tension	1150:1184	tension	1178:1184	Captive bubble surfactometry showed an increase in minimum and maximum surface tension and percent surface area compression, as well as a higher compressibility and hysteresis in MPS IIIA surfactant upon dynamic cycling.
33918094	9	35	from	compressibility	1244:1258	arg1	surfactant					1287:1296	MPS IIIA surfactant	1278:1296	MPS IIIA surfactant	1278:1296	Captive bubble surfactometry showed an increase in minimum and maximum surface tension and percent surface area compression, as well as a higher compressibility and hysteresis in MPS IIIA surfactant upon dynamic cycling.
33918094	9	35	from	compressibility	1244:1258	arg1	tension					1178:1184	minimum and maximum surface tension	1150:1184	tension	1178:1184	Captive bubble surfactometry showed an increase in minimum and maximum surface tension and percent surface area compression, as well as a higher compressibility and hysteresis in MPS IIIA surfactant upon dynamic cycling.
33918094	9	35	from	compressibility	1244:1258	arg1	area					1206:1209	percent surface area	1190:1209	percent surface area	1190:1209	Captive bubble surfactometry showed an increase in minimum and maximum surface tension and percent surface area compression, as well as a higher compressibility and hysteresis in MPS IIIA surfactant upon dynamic cycling.
33918094	4	36	theme	lavage	563:568	arg1	BALF					577:580	BALF	577:580	BALF	577:580	We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age).
33918094	4	36	theme	lavage	563:568	arg1	fluid					570:574	bronchoalveolar lavage fluid	547:574	bronchoalveolar lavage fluid (BALF)	547:581	We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age).
33918094	0	37	theme	Pulmonary	48:56	arg1	Amount					69:74	Pulmonary Surfactant Amount	48:74	Pulmonary Surfactant Amount	48:74	Increased Alveolar Heparan Sulphate and Reduced Pulmonary Surfactant Amount and Function in the Mucopolysaccharidosis IIIA Mouse.
33918094	9	38	theme	surface	1198:1204	arg1	area					1206:1209	percent surface area	1190:1209	percent surface area	1190:1209	Captive bubble surfactometry showed an increase in minimum and maximum surface tension and percent surface area compression, as well as a higher compressibility and hysteresis in MPS IIIA surfactant upon dynamic cycling.
33918094	1	39	with	disease	191:197	arg1	pathologies					242:252	significant neurological and skeletal pathologies	204:252	significant neurological and skeletal pathologies	204:252	Mucopolysaccharidosis IIIA (MPS IIIA) is a lysosomal storage disease with significant neurological and skeletal pathologies.
33918094	8	40	from	protein	1027:1033	arg1	tissue					1038:1043	tissue	1038:1043	tissue	1038:1043	There was a reduction in SP-A, -C and -D mRNA, SP-D protein in tissue and SP-A, -C and -D protein in BALF of MPS IIIA mice.
33918094	4	41	theme	pulmonary	526:534	arg1	tissue					536:541	pulmonary tissue	526:541	pulmonary tissue	526:541	We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age).
33918094	1	42	theme	skeletal	233:240	arg1	pathologies					242:252	significant neurological and skeletal pathologies	204:252	significant neurological and skeletal pathologies	204:252	Mucopolysaccharidosis IIIA (MPS IIIA) is a lysosomal storage disease with significant neurological and skeletal pathologies.
33918094	10	43	theme	alveolar	1378:1385	arg1	surfactant					1387:1396	alveolar surfactant	1378:1396	alveolar surfactant	1378:1396	Collectively these biochemical and biophysical changes in alveolar surfactant are likely to be detrimental to lung function in MPS IIIA.
33918094	9	44	dep	tension	1178:1184	arg1	compression					1211:1221	compression	1211:1221	compression	1211:1221	Captive bubble surfactometry showed an increase in minimum and maximum surface tension and percent surface area compression, as well as a higher compressibility and hysteresis in MPS IIIA surfactant upon dynamic cycling.
33918094	6	45	theme	cholesteryl	824:834	arg1	esters					836:841	cholesteryl esters	824:841	cholesteryl esters	824:841	There was an increase in HS and a decrease in BMP and cholesteryl esters (CE) in MPS IIIA BALF.
33918094	6	46	from	decrease	804:811	arg1	esters					836:841	cholesteryl esters	824:841	cholesteryl esters	824:841	There was an increase in HS and a decrease in BMP and cholesteryl esters (CE) in MPS IIIA BALF.
33918094	6	46	from	decrease	804:811	arg1	CE					844:845	CE	844:845	CE	844:845	There was an increase in HS and a decrease in BMP and cholesteryl esters (CE) in MPS IIIA BALF.
33918094	6	46	from	decrease	804:811	arg1	BMP					816:818	BMP	816:818	BMP	816:818	There was an increase in HS and a decrease in BMP and cholesteryl esters (CE) in MPS IIIA BALF.
33918094	6	46	from	decrease	804:811	arg1	HS					795:796	HS	795:796	HS	795:796	There was an increase in HS and a decrease in BMP and cholesteryl esters (CE) in MPS IIIA BALF.
33918094	4	47	from	proteins	509:516	arg1	tissue					536:541	pulmonary tissue	526:541	pulmonary tissue	526:541	We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age).
33918094	4	47	from	proteins	509:516	arg1	mice					632:635	healthy and diseased mice	611:635	healthy and diseased mice (20 weeks of age)	611:653	We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age).
33918094	4	47	from	proteins	509:516	arg1	BALF					577:580	BALF	577:580	BALF	577:580	We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age).
33918094	4	47	from	proteins	509:516	arg1	fluid					570:574	bronchoalveolar lavage fluid	547:574	bronchoalveolar lavage fluid (BALF)	547:581	We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age).
33918094	9	48	theme	higher	1237:1242	arg1	compressibility					1244:1258	a higher compressibility	1235:1258	a higher compressibility	1235:1258	Captive bubble surfactometry showed an increase in minimum and maximum surface tension and percent surface area compression, as well as a higher compressibility and hysteresis in MPS IIIA surfactant upon dynamic cycling.
33918094	6	49	from	BMP	816:818	arg1	BALF					860:863	MPS IIIA BALF	851:863	MPS IIIA BALF	851:863	There was an increase in HS and a decrease in BMP and cholesteryl esters (CE) in MPS IIIA BALF.
33918094	8	50	theme	SP-D	1022:1025	arg1	protein					1027:1033	SP-D protein	1022:1033	SP-D protein in tissue	1022:1043	There was a reduction in SP-A, -C and -D mRNA, SP-D protein in tissue and SP-A, -C and -D protein in BALF of MPS IIIA mice.
33918094	1	51	theme	storage	183:189	arg1	IIIA					152:155	Mucopolysaccharidosis IIIA	130:155	Mucopolysaccharidosis IIIA (MPS IIIA)	130:166	Mucopolysaccharidosis IIIA (MPS IIIA) is a lysosomal storage disease with significant neurological and skeletal pathologies.
33918094	1	51	theme	storage	183:189	arg1	disease					191:197	a lysosomal storage disease	171:197	a lysosomal storage disease with significant neurological and skeletal pathologies	171:252	Mucopolysaccharidosis IIIA (MPS IIIA) is a lysosomal storage disease with significant neurological and skeletal pathologies.
33918094	10	52	theme	lung	1430:1433	arg1	function					1435:1442	lung function	1430:1442	lung function in MPS IIIA	1430:1454	Collectively these biochemical and biophysical changes in alveolar surfactant are likely to be detrimental to lung function in MPS IIIA.
33918094	2	53	theme	MPS	333:335	arg1	patients					342:349	MPS IIIA patients	333:349	MPS IIIA patients	333:349	Respiratory dysfunction is a secondary pathology contributing to mortality in MPS IIIA patients.
33918094	9	54	theme	IIIA	1282:1285	arg1	surfactant					1287:1296	MPS IIIA surfactant	1278:1296	MPS IIIA surfactant	1278:1296	Captive bubble surfactometry showed an increase in minimum and maximum surface tension and percent surface area compression, as well as a higher compressibility and hysteresis in MPS IIIA surfactant upon dynamic cycling.
33918094	9	55	from	increase	1138:1145	arg1	surfactant					1287:1296	MPS IIIA surfactant	1278:1296	MPS IIIA surfactant	1278:1296	Captive bubble surfactometry showed an increase in minimum and maximum surface tension and percent surface area compression, as well as a higher compressibility and hysteresis in MPS IIIA surfactant upon dynamic cycling.
33918094	9	55	from	increase	1138:1145	arg1	tension					1178:1184	minimum and maximum surface tension	1150:1184	tension	1178:1184	Captive bubble surfactometry showed an increase in minimum and maximum surface tension and percent surface area compression, as well as a higher compressibility and hysteresis in MPS IIIA surfactant upon dynamic cycling.
33918094	9	55	from	increase	1138:1145	arg1	area					1206:1209	percent surface area	1190:1209	percent surface area	1190:1209	Captive bubble surfactometry showed an increase in minimum and maximum surface tension and percent surface area compression, as well as a higher compressibility and hysteresis in MPS IIIA surfactant upon dynamic cycling.
33918094	4	56	theme	healthy	611:617	arg1	mice					632:635	healthy and diseased mice	611:635	healthy and diseased mice (20 weeks of age)	611:653	We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age).
33918094	5	57	theme	ganglioside	674:684	arg1	GM3					686:688	ganglioside GM3	674:688	ganglioside GM3	674:688	Heparan sulphate, ganglioside GM3 and bis(monoacylglycero)phosphate (BMP) were increased in MPS IIIA lung tissue.
33918094	2	58	theme	Respiratory	255:265	arg1	pathology					294:302	a secondary pathology	282:302	a secondary pathology contributing to mortality in MPS IIIA patients	282:349	Respiratory dysfunction is a secondary pathology contributing to mortality in MPS IIIA patients.
33918094	2	58	theme	Respiratory	255:265	arg1	dysfunction					267:277	Respiratory dysfunction	255:277	Respiratory dysfunction	255:277	Respiratory dysfunction is a secondary pathology contributing to mortality in MPS IIIA patients.
33918094	1	59	theme	neurological	216:227	arg1	pathologies					242:252	significant neurological and skeletal pathologies	204:252	significant neurological and skeletal pathologies	204:252	Mucopolysaccharidosis IIIA (MPS IIIA) is a lysosomal storage disease with significant neurological and skeletal pathologies.
33918094	6	60	theme	MPS	851:853	arg1	BALF					860:863	MPS IIIA BALF	851:863	MPS IIIA BALF	851:863	There was an increase in HS and a decrease in BMP and cholesteryl esters (CE) in MPS IIIA BALF.
33918094	4	61	theme	diseased	623:630	arg1	mice					632:635	healthy and diseased mice	611:635	healthy and diseased mice (20 weeks of age)	611:653	We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age).
33918094	2	62	theme	secondary	284:292	arg1	pathology					294:302	a secondary pathology	282:302	a secondary pathology contributing to mortality in MPS IIIA patients	282:349	Respiratory dysfunction is a secondary pathology contributing to mortality in MPS IIIA patients.
33918094	2	62	theme	secondary	284:292	arg1	dysfunction					267:277	Respiratory dysfunction	255:277	Respiratory dysfunction	255:277	Respiratory dysfunction is a secondary pathology contributing to mortality in MPS IIIA patients.
33918094	8	63	from	protein	1065:1071	arg1	tissue					1038:1043	tissue	1038:1043	tissue	1038:1043	There was a reduction in SP-A, -C and -D mRNA, SP-D protein in tissue and SP-A, -C and -D protein in BALF of MPS IIIA mice.
33918094	1	64	theme	MPS	158:160	arg1	IIIA					152:155	Mucopolysaccharidosis IIIA	130:155	Mucopolysaccharidosis IIIA (MPS IIIA)	130:166	Mucopolysaccharidosis IIIA (MPS IIIA) is a lysosomal storage disease with significant neurological and skeletal pathologies.
33918094	1	64	theme	MPS	158:160	arg1	IIIA					162:165	MPS IIIA	158:165	MPS IIIA	158:165	Mucopolysaccharidosis IIIA (MPS IIIA) is a lysosomal storage disease with significant neurological and skeletal pathologies.
33918094	10	65	from	changes	1367:1373	arg1	surfactant					1387:1396	alveolar surfactant	1378:1396	alveolar surfactant	1378:1396	Collectively these biochemical and biophysical changes in alveolar surfactant are likely to be detrimental to lung function in MPS IIIA.
33918094	4	66	theme	age	650:652	arg1	weeks					641:645	20 weeks	638:645	20 weeks of age	638:652	We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age).
33918094	9	67	theme	bubble	1107:1112	arg1	surfactometry					1114:1126	Captive bubble surfactometry	1099:1126	Captive bubble surfactometry	1099:1126	Captive bubble surfactometry showed an increase in minimum and maximum surface tension and percent surface area compression, as well as a higher compressibility and hysteresis in MPS IIIA surfactant upon dynamic cycling.
33918094	3	68	theme	Pulmonary	352:360	arg1	surfactant					362:371	Pulmonary surfactant	352:371	Pulmonary surfactant	352:371	Pulmonary surfactant is crucial to optimal lung function and has not been investigated in MPS IIIA.
33918094	0	69	theme	Mucopolysaccharidosis	96:116	arg1	Mouse					123:127	the Mucopolysaccharidosis IIIA Mouse	92:127	the Mucopolysaccharidosis IIIA Mouse	92:127	Increased Alveolar Heparan Sulphate and Reduced Pulmonary Surfactant Amount and Function in the Mucopolysaccharidosis IIIA Mouse.
33918094	7	70	theme	Phospholipid	866:877	arg1	composition					879:889	Phospholipid composition	866:889	Phospholipid composition	866:889	Phospholipid composition remained unchanged, but BALF total phospholipids were reduced (49.70%) in MPS IIIA.
33918094	8	71	from	reduction	987:995	arg1	BALF					1076:1079	BALF	1076:1079	BALF of MPS IIIA mice	1076:1096	There was a reduction in SP-A, -C and -D mRNA, SP-D protein in tissue and SP-A, -C and -D protein in BALF of MPS IIIA mice.
33918094	8	71	from	reduction	987:995	arg1	protein					1065:1071	SP-A, -C and -D protein	1049:1071	SP-A, -C and -D protein	1049:1071	There was a reduction in SP-A, -C and -D mRNA, SP-D protein in tissue and SP-A, -C and -D protein in BALF of MPS IIIA mice.
33918094	8	71	from	reduction	987:995	arg1	mRNA					1016:1019	SP-A, -C and -D mRNA	1000:1019	SP-A, -C and -D mRNA	1000:1019	There was a reduction in SP-A, -C and -D mRNA, SP-D protein in tissue and SP-A, -C and -D protein in BALF of MPS IIIA mice.
33918094	8	71	from	reduction	987:995	arg1	protein					1027:1033	SP-D protein	1022:1033	SP-D protein in tissue	1022:1043	There was a reduction in SP-A, -C and -D mRNA, SP-D protein in tissue and SP-A, -C and -D protein in BALF of MPS IIIA mice.
33918094	3	72	theme	lung	395:398	arg1	function					400:407	optimal lung function	387:407	optimal lung function	387:407	Pulmonary surfactant is crucial to optimal lung function and has not been investigated in MPS IIIA.
33918094	8	73	theme	MPS	1084:1086	arg1	mice					1093:1096	MPS IIIA mice	1084:1096	MPS IIIA mice	1084:1096	There was a reduction in SP-A, -C and -D mRNA, SP-D protein in tissue and SP-A, -C and -D protein in BALF of MPS IIIA mice.
33918094	5	74	theme	MPS	748:750	arg1	tissue					762:767	MPS IIIA lung tissue	748:767	MPS IIIA lung tissue	748:767	Heparan sulphate, ganglioside GM3 and bis(monoacylglycero)phosphate (BMP) were increased in MPS IIIA lung tissue.
33918094	9	75	theme	minimum	1150:1156	arg1	tension					1178:1184	minimum and maximum surface tension	1150:1184	tension	1178:1184	Captive bubble surfactometry showed an increase in minimum and maximum surface tension and percent surface area compression, as well as a higher compressibility and hysteresis in MPS IIIA surfactant upon dynamic cycling.
33918094	4	76	from	sulphate	472:479	arg1	tissue					536:541	pulmonary tissue	526:541	pulmonary tissue	526:541	We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age).
33918094	4	76	from	sulphate	472:479	arg1	mice					632:635	healthy and diseased mice	611:635	healthy and diseased mice (20 weeks of age)	611:653	We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age).
33918094	4	76	from	sulphate	472:479	arg1	BALF					577:580	BALF	577:580	BALF	577:580	We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age).
33918094	4	76	from	sulphate	472:479	arg1	fluid					570:574	bronchoalveolar lavage fluid	547:574	bronchoalveolar lavage fluid (BALF)	547:581	We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age).
33918094	4	77	theme	bronchoalveolar	547:561	arg1	lavage					563:568	bronchoalveolar lavage	547:568	bronchoalveolar lavage fluid (BALF)	547:581	We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age).
33918094	5	78	theme	lung	757:760	arg1	tissue					762:767	MPS IIIA lung tissue	748:767	MPS IIIA lung tissue	748:767	Heparan sulphate, ganglioside GM3 and bis(monoacylglycero)phosphate (BMP) were increased in MPS IIIA lung tissue.
33918094	9	79	theme	maximum	1162:1168	arg1	tension					1178:1184	minimum and maximum surface tension	1150:1184	tension	1178:1184	Captive bubble surfactometry showed an increase in minimum and maximum surface tension and percent surface area compression, as well as a higher compressibility and hysteresis in MPS IIIA surfactant upon dynamic cycling.
33918094	4	80	dep	mice	632:635	arg1	weeks					641:645	20 weeks	638:645	20 weeks of age	638:652	We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age).
33918094	3	81	theme	MPS	442:444	arg1	IIIA					446:449	MPS IIIA	442:449	MPS IIIA	442:449	Pulmonary surfactant is crucial to optimal lung function and has not been investigated in MPS IIIA.
33918094	0	82	theme	Surfactant	58:67	arg1	Amount					69:74	Pulmonary Surfactant Amount	48:74	Pulmonary Surfactant Amount	48:74	Increased Alveolar Heparan Sulphate and Reduced Pulmonary Surfactant Amount and Function in the Mucopolysaccharidosis IIIA Mouse.
33918094	9	83	theme	percent	1190:1196	arg1	area					1206:1209	percent surface area	1190:1209	percent surface area	1190:1209	Captive bubble surfactometry showed an increase in minimum and maximum surface tension and percent surface area compression, as well as a higher compressibility and hysteresis in MPS IIIA surfactant upon dynamic cycling.
33918094	10	84	theme	biophysical	1355:1365	arg1	changes					1367:1373	these biochemical and biophysical changes	1333:1373	these biochemical and biophysical changes in alveolar surfactant	1333:1396	Collectively these biochemical and biophysical changes in alveolar surfactant are likely to be detrimental to lung function in MPS IIIA.
33918094	8	85	theme	SP-A	1000:1003	arg1	mRNA					1016:1019	SP-A, -C and -D mRNA	1000:1019	SP-A, -C and -D mRNA	1000:1019	There was a reduction in SP-A, -C and -D mRNA, SP-D protein in tissue and SP-A, -C and -D protein in BALF of MPS IIIA mice.
33918094	4	86	theme	surfactant	588:597	arg1	activity					599:606	surfactant activity	588:606	surfactant activity in healthy and diseased mice (20 weeks of age)	588:653	We measured heparan sulphate (HS), lipids and surfactant proteins (SP) in pulmonary tissue and bronchoalveolar lavage fluid (BALF), and surfactant activity in healthy and diseased mice (20 weeks of age).
33918094	10	87	theme	biochemical	1339:1349	arg1	changes					1367:1373	these biochemical and biophysical changes	1333:1373	these biochemical and biophysical changes in alveolar surfactant	1333:1396	Collectively these biochemical and biophysical changes in alveolar surfactant are likely to be detrimental to lung function in MPS IIIA.
33918094	8	88	theme	-C	1006:1007	arg1	mRNA					1016:1019	SP-A, -C and -D mRNA	1000:1019	SP-A, -C and -D mRNA	1000:1019	There was a reduction in SP-A, -C and -D mRNA, SP-D protein in tissue and SP-A, -C and -D protein in BALF of MPS IIIA mice.
33918094	8	89	from	mRNA	1016:1019	arg1	tissue					1038:1043	tissue	1038:1043	tissue	1038:1043	There was a reduction in SP-A, -C and -D mRNA, SP-D protein in tissue and SP-A, -C and -D protein in BALF of MPS IIIA mice.
33918094	8	90	theme	mice	1093:1096	arg1	BALF					1076:1079	BALF	1076:1079	BALF of MPS IIIA mice	1076:1096	There was a reduction in SP-A, -C and -D mRNA, SP-D protein in tissue and SP-A, -C and -D protein in BALF of MPS IIIA mice.
33918094	0	91	theme	Alveolar	10:17	arg1	Sulphate					27:34	Increased Alveolar Heparan Sulphate	0:34	Increased Alveolar Heparan Sulphate	0:34	Increased Alveolar Heparan Sulphate and Reduced Pulmonary Surfactant Amount and Function in the Mucopolysaccharidosis IIIA Mouse.
33480836	2	0	from	Karelinea	158:166	arg1	China					235:239	China	235:239	China	235:239	A bacterial strain, designated TRM 80801T, was isolated from the Karelinea in Taklamakan desert, Xinjiang Uygur Autonomous Region, north-west China.
33480836	2	0	from	Karelinea	158:166	arg1	Uygur					199:203	Uygur	199:203	Uygur	199:203	A bacterial strain, designated TRM 80801T, was isolated from the Karelinea in Taklamakan desert, Xinjiang Uygur Autonomous Region, north-west China.
33480836	3	1	dep	Gram-stain-positive	253:271	arg1	short					295:299	short	295:299	short	295:299	Cells were Gram-stain-positive, aerobic, non-motile, short rods.
33480836	3	1	dep	Gram-stain-positive	253:271	arg1	non-motile					283:292	non-motile	283:292	non-motile	283:292	Cells were Gram-stain-positive, aerobic, non-motile, short rods.
33480836	3	1	dep	Gram-stain-positive	253:271	arg1	aerobic					274:280	aerobic	274:280	aerobic	274:280	Cells were Gram-stain-positive, aerobic, non-motile, short rods.
33480836	5	2	theme	YZYP	585:588	arg1	306T					590:593	Microbacterium suaedae YZYP 306T	562:593	Microbacterium suaedae YZYP 306T (98.97 %)	562:603	Phylogenetic analyses of the 16S rRNA gene sequences placed strain TRM 80801T within the genus Microbacterium with the highest similarities to Microbacterium suaedae YZYP 306T (98.97 %) and Microbacterium indicum BBH6T (98.17 %), respectively.
33480836	5	2	theme	YZYP	585:588	arg1	%					602:602	98.97 %	596:602	98.97 %	596:602	Phylogenetic analyses of the 16S rRNA gene sequences placed strain TRM 80801T within the genus Microbacterium with the highest similarities to Microbacterium suaedae YZYP 306T (98.97 %) and Microbacterium indicum BBH6T (98.17 %), respectively.
33480836	5	3	theme	gene	457:460	arg1	sequences					462:470	the 16S rRNA gene sequences	444:470	the 16S rRNA gene sequences	444:470	Phylogenetic analyses of the 16S rRNA gene sequences placed strain TRM 80801T within the genus Microbacterium with the highest similarities to Microbacterium suaedae YZYP 306T (98.97 %) and Microbacterium indicum BBH6T (98.17 %), respectively.
33480836	14	4	theme	name	1473:1476	arg1	sp					1504:1505	the name Microbacterium karelineae sp	1469:1505	the name Microbacterium karelineae sp	1469:1505	On the basis of the evidence presented in this study, strain TRM 80801T is representative of a novel species in the genus Microbacterium, for which the name Microbacterium karelineae sp.
33480836	4	5	dep	%	405:405	arg1	w/v					408:410	w/v	408:410	w/v	408:410	Strain TRM 80801T grew at 4-50 °C, with optimum growth at 28 °C, and grew at pH 6.0-11.0 and 1-15 % (w/v) NaCl.
33480836	13	6	dep	Microbacterium	1277:1290	arg1	suaedae					1292:1298	suaedae	1292:1298	suaedae	1292:1298	The whole-genome average nucleotide identity (ANI) value between strain TRM 80801T and Microbacterium suaedae YZYP 306T is 70.2 %.
33480836	14	7	theme	karelineae	1493:1502	arg1	sp					1504:1505	the name Microbacterium karelineae sp	1469:1505	the name Microbacterium karelineae sp	1469:1505	On the basis of the evidence presented in this study, strain TRM 80801T is representative of a novel species in the genus Microbacterium, for which the name Microbacterium karelineae sp.
33480836	16	8	theme	2019248T=KCTC	1567:1579	arg1	49357T					1581:1586	=CCTCC AB 2019248T=KCTC 49357T	1557:1586	=CCTCC AB 2019248T=KCTC 49357T	1557:1586	The type strain is TRM 80801T (=CCTCC AB 2019248T=KCTC 49357T).
33480836	16	8	theme	2019248T=KCTC	1567:1579	arg1	80801T					1549:1554	TRM 80801T	1545:1554	TRM 80801T (=CCTCC AB 2019248T=KCTC 49357T)	1545:1587	The type strain is TRM 80801T (=CCTCC AB 2019248T=KCTC 49357T).
33480836	2	9	theme	bacterial	95:103	arg1	strain					105:110	A bacterial strain	93:110	A bacterial strain	93:110	A bacterial strain, designated TRM 80801T, was isolated from the Karelinea in Taklamakan desert, Xinjiang Uygur Autonomous Region, north-west China.
33480836	8	10	theme	acyl	860:863	arg1	glycolyl					895:902	glycolyl	895:902	glycolyl	895:902	The acyl type of the peptidoglycan was glycolyl.
33480836	8	10	theme	acyl	860:863	arg1	type					865:868	The acyl type	856:868	The acyl type of the peptidoglycan	856:889	The acyl type of the peptidoglycan was glycolyl.
33480836	1	11	theme	halophyte	51:59	arg1	plant					61:65	a halophyte plant	49:65	a halophyte plant in the Taklamakan desert	49:90	nov. isolated from a halophyte plant in the Taklamakan desert.
33480836	1	12	from	plant	61:65	arg1	desert					85:90	the Taklamakan desert	70:90	the Taklamakan desert	70:90	nov. isolated from a halophyte plant in the Taklamakan desert.
33480836	13	13	theme	whole-genome	1194:1205	arg1	%					1318:1318	70.2 %	1313:1318	70.2 %	1313:1318	The whole-genome average nucleotide identity (ANI) value between strain TRM 80801T and Microbacterium suaedae YZYP 306T is 70.2 %.
33480836	13	13	theme	whole-genome	1194:1205	arg1	value					1241:1245	The whole-genome average nucleotide identity (ANI) value	1190:1245	The whole-genome average nucleotide identity (ANI) value between strain TRM 80801T and Microbacterium suaedae YZYP 306T	1190:1308	The whole-genome average nucleotide identity (ANI) value between strain TRM 80801T and Microbacterium suaedae YZYP 306T is 70.2 %.
33480836	5	14	dep	Microbacterium	609:622	arg1	indicum					624:630	indicum	624:630	indicum	624:630	Phylogenetic analyses of the 16S rRNA gene sequences placed strain TRM 80801T within the genus Microbacterium with the highest similarities to Microbacterium suaedae YZYP 306T (98.97 %) and Microbacterium indicum BBH6T (98.17 %), respectively.
33480836	10	15	theme	cellular	988:995	arg1	anteiso-C15 					1014:1025	anteiso-C15 	1014:1025	anteiso-C15 	1014:1025	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
33480836	10	15	theme	cellular	988:995	arg1	acids					1003:1007	The major cellular fatty acids	978:1007	The major cellular fatty acids	978:1007	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
33480836	6	16	theme	DNA	667:669	arg1	content					675:681	The DNA G+C content	663:681	The DNA G+C content of TRM 80801T	663:695	The DNA G+C content of TRM 80801T is 69.38 mol%.
33480836	6	16	theme	DNA	667:669	arg1	%					709:709	69.38 mol%	700:709	69.38 mol%	700:709	The DNA G+C content of TRM 80801T is 69.38 mol%.
33480836	6	17	theme	69.38 mol	700:708	arg1	content					675:681	The DNA G+C content	663:681	The DNA G+C content of TRM 80801T	663:695	The DNA G+C content of TRM 80801T is 69.38 mol%.
33480836	6	17	theme	69.38 mol	700:708	arg1	%					709:709	69.38 mol%	700:709	69.38 mol%	700:709	The DNA G+C content of TRM 80801T is 69.38 mol%.
33480836	4	18	from	28 °C	365:369	arg1	growth					355:360	optimum growth	347:360	optimum growth at 28 °C	347:369	Strain TRM 80801T grew at 4-50 °C, with optimum growth at 28 °C, and grew at pH 6.0-11.0 and 1-15 % (w/v) NaCl.
33480836	13	19	theme	average	1207:1213	arg1	identity					1226:1233	average nucleotide identity	1207:1233	The whole-genome average nucleotide identity (ANI) value between strain TRM 80801T and Microbacterium suaedae YZYP 306T	1190:1308	The whole-genome average nucleotide identity (ANI) value between strain TRM 80801T and Microbacterium suaedae YZYP 306T is 70.2 %.
33480836	13	19	theme	average	1207:1213	arg1	ANI					1236:1238	ANI	1236:1238	ANI	1236:1238	The whole-genome average nucleotide identity (ANI) value between strain TRM 80801T and Microbacterium suaedae YZYP 306T is 70.2 %.
33480836	8	20	theme	peptidoglycan	877:889	arg1	glycolyl					895:902	glycolyl	895:902	glycolyl	895:902	The acyl type of the peptidoglycan was glycolyl.
33480836	8	20	theme	peptidoglycan	877:889	arg1	type					865:868	The acyl type	856:868	The acyl type of the peptidoglycan	856:889	The acyl type of the peptidoglycan was glycolyl.
33480836	5	21	theme	highest	538:544	arg1	similarities					546:557	the highest similarities	534:557	the highest similarities to Microbacterium suaedae YZYP 306T (98.97 %) and Microbacterium indicum BBH6T (98.17 %), respectively	534:660	Phylogenetic analyses of the 16S rRNA gene sequences placed strain TRM 80801T within the genus Microbacterium with the highest similarities to Microbacterium suaedae YZYP 306T (98.97 %) and Microbacterium indicum BBH6T (98.17 %), respectively.
33480836	14	22	dep	evidence	1341:1348	arg1	the					1324:1326	the	1324:1326	the	1324:1326	On the basis of the evidence presented in this study, strain TRM 80801T is representative of a novel species in the genus Microbacterium, for which the name Microbacterium karelineae sp.
33480836	14	22	dep	evidence	1341:1348	arg1	basis					1328:1332	basis	1328:1332	basis	1328:1332	On the basis of the evidence presented in this study, strain TRM 80801T is representative of a novel species in the genus Microbacterium, for which the name Microbacterium karelineae sp.
33480836	5	23	theme	Phylogenetic	419:430	arg1	analyses					432:439	Phylogenetic analyses	419:439	Phylogenetic analyses of the 16S rRNA gene sequences	419:470	Phylogenetic analyses of the 16S rRNA gene sequences placed strain TRM 80801T within the genus Microbacterium with the highest similarities to Microbacterium suaedae YZYP 306T (98.97 %) and Microbacterium indicum BBH6T (98.17 %), respectively.
33480836	14	24	theme	TRM	1382:1384	arg1	80801T					1386:1391	strain TRM 80801T	1375:1391	strain TRM 80801T	1375:1391	On the basis of the evidence presented in this study, strain TRM 80801T is representative of a novel species in the genus Microbacterium, for which the name Microbacterium karelineae sp.
33480836	10	25	dep	anteiso-C15 	1014:1025	arg1	 0					1060:1061	 0	1060:1061	 0	1060:1061	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
33480836	10	25	dep	anteiso-C15 	1014:1025	arg1	iso-C16 					1051:1058	iso-C16 	1051:1058	iso-C16 	1051:1058	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
33480836	10	25	dep	anteiso-C15 	1014:1025	arg1	 0					1044:1045	 0	1044:1045	 0	1044:1045	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
33480836	10	25	dep	anteiso-C15 	1014:1025	arg1	 0					1027:1028	 0	1027:1028	 0	1027:1028	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
33480836	6	26	theme	80801T	690:695	arg1	content					675:681	The DNA G+C content	663:681	The DNA G+C content of TRM 80801T	663:695	The DNA G+C content of TRM 80801T is 69.38 mol%.
33480836	6	26	theme	80801T	690:695	arg1	%					709:709	69.38 mol%	700:709	69.38 mol%	700:709	The DNA G+C content of TRM 80801T is 69.38 mol%.
33480836	11	27	theme	predominant	1068:1078	arg1	MK-10					1098:1102	MK-10	1098:1102	MK-10	1098:1102	The predominant menaquinones were MK-10, MK-11 and MK-12.
33480836	11	27	theme	predominant	1068:1078	arg1	menaquinones					1080:1091	The predominant menaquinones	1064:1091	The predominant menaquinones	1064:1091	The predominant menaquinones were MK-10, MK-11 and MK-12.
33480836	0	28	theme	karelineae	15:24	arg1	sp					26:27	Microbacterium karelineae sp	0:27	Microbacterium karelineae sp.	0:28	Microbacterium karelineae sp.
33480836	2	29	theme	TRM	124:126	arg1	80801T					128:133	TRM 80801T	124:133	TRM 80801T	124:133	A bacterial strain, designated TRM 80801T, was isolated from the Karelinea in Taklamakan desert, Xinjiang Uygur Autonomous Region, north-west China.
33480836	5	30	theme	Microbacterium	562:575	arg1	306T					590:593	Microbacterium suaedae YZYP 306T	562:593	Microbacterium suaedae YZYP 306T (98.97 %)	562:603	Phylogenetic analyses of the 16S rRNA gene sequences placed strain TRM 80801T within the genus Microbacterium with the highest similarities to Microbacterium suaedae YZYP 306T (98.97 %) and Microbacterium indicum BBH6T (98.17 %), respectively.
33480836	5	30	theme	Microbacterium	562:575	arg1	%					602:602	98.97 %	596:602	98.97 %	596:602	Phylogenetic analyses of the 16S rRNA gene sequences placed strain TRM 80801T within the genus Microbacterium with the highest similarities to Microbacterium suaedae YZYP 306T (98.97 %) and Microbacterium indicum BBH6T (98.17 %), respectively.
33480836	6	31	theme	TRM	686:688	arg1	80801T					690:695	TRM 80801T	686:695	TRM 80801T	686:695	The DNA G+C content of TRM 80801T is 69.38 mol%.
33480836	1	32	theme	Taklamakan	74:83	arg1	desert					85:90	the Taklamakan desert	70:90	the Taklamakan desert	70:90	nov. isolated from a halophyte plant in the Taklamakan desert.
33480836	7	33	theme	amino	754:758	arg1	acid					786:789	glutamic acid	777:789	glutamic acid	777:789	The cell-wall peptidoglycan contained the amino acids ornithine, glutamic acid, glycine and alanine, the diagnostic diamino acid was ornithine.
33480836	7	33	theme	amino	754:758	arg1	alanine					804:810	alanine	804:810	alanine	804:810	The cell-wall peptidoglycan contained the amino acids ornithine, glutamic acid, glycine and alanine, the diagnostic diamino acid was ornithine.
33480836	7	33	theme	amino	754:758	arg1	acids					760:764	the amino acids ornithine	750:774	the amino acids ornithine	750:774	The cell-wall peptidoglycan contained the amino acids ornithine, glutamic acid, glycine and alanine, the diagnostic diamino acid was ornithine.
33480836	7	33	theme	amino	754:758	arg1	glycine					792:798	glycine	792:798	glycine	792:798	The cell-wall peptidoglycan contained the amino acids ornithine, glutamic acid, glycine and alanine, the diagnostic diamino acid was ornithine.
33480836	0	34	theme	Microbacterium	0:13	arg1	sp					26:27	Microbacterium karelineae sp	0:27	Microbacterium karelineae sp.	0:28	Microbacterium karelineae sp.
33480836	13	35	theme	TRM	1262:1264	arg1	80801T					1266:1271	strain TRM 80801T	1255:1271	strain TRM 80801T	1255:1271	The whole-genome average nucleotide identity (ANI) value between strain TRM 80801T and Microbacterium suaedae YZYP 306T is 70.2 %.
33480836	14	36	theme	strain	1375:1380	arg1	80801T					1386:1391	strain TRM 80801T	1375:1391	strain TRM 80801T	1375:1391	On the basis of the evidence presented in this study, strain TRM 80801T is representative of a novel species in the genus Microbacterium, for which the name Microbacterium karelineae sp.
33480836	4	37	theme	optimum	347:353	arg1	growth					355:360	optimum growth	347:360	optimum growth at 28 °C	347:369	Strain TRM 80801T grew at 4-50 °C, with optimum growth at 28 °C, and grew at pH 6.0-11.0 and 1-15 % (w/v) NaCl.
33480836	16	38	theme	type	1530:1533	arg1	strain					1535:1540	The type strain	1526:1540	The type strain	1526:1540	The type strain is TRM 80801T (=CCTCC AB 2019248T=KCTC 49357T).
33480836	16	38	theme	type	1530:1533	arg1	80801T					1549:1554	TRM 80801T	1545:1554	TRM 80801T (=CCTCC AB 2019248T=KCTC 49357T)	1545:1587	The type strain is TRM 80801T (=CCTCC AB 2019248T=KCTC 49357T).
33480836	13	39	theme	Microbacterium	1277:1290	arg1	306T					1305:1308	Microbacterium suaedae YZYP 306T	1277:1308	Microbacterium suaedae YZYP 306T	1277:1308	The whole-genome average nucleotide identity (ANI) value between strain TRM 80801T and Microbacterium suaedae YZYP 306T is 70.2 %.
33480836	3	40	theme	Gram-stain-positive	253:271	arg1	rods					301:304	Gram-stain-positive, aerobic, non-motile, short rods	253:304	Gram-stain-positive, aerobic, non-motile, short rods	253:304	Cells were Gram-stain-positive, aerobic, non-motile, short rods.
33480836	5	41	theme	genus	508:512	arg1	Microbacterium					514:527	the genus Microbacterium	504:527	the genus Microbacterium with the highest similarities to Microbacterium suaedae YZYP 306T (98.97 %) and Microbacterium indicum BBH6T (98.17 %), respectively	504:660	Phylogenetic analyses of the 16S rRNA gene sequences placed strain TRM 80801T within the genus Microbacterium with the highest similarities to Microbacterium suaedae YZYP 306T (98.97 %) and Microbacterium indicum BBH6T (98.17 %), respectively.
33480836	2	42	dep	Uygur	199:203	arg1	Xinjiang					190:197	Xinjiang	190:197	Xinjiang	190:197	A bacterial strain, designated TRM 80801T, was isolated from the Karelinea in Taklamakan desert, Xinjiang Uygur Autonomous Region, north-west China.
33480836	2	42	dep	Uygur	199:203	arg1	Region					216:221	Autonomous Region	205:221	Autonomous Region	205:221	A bacterial strain, designated TRM 80801T, was isolated from the Karelinea in Taklamakan desert, Xinjiang Uygur Autonomous Region, north-west China.
33480836	14	43	theme	species	1422:1428	arg1	representative					1396:1409	representative	1396:1409	representative	1396:1409	On the basis of the evidence presented in this study, strain TRM 80801T is representative of a novel species in the genus Microbacterium, for which the name Microbacterium karelineae sp.
33480836	7	44	dep	acids	760:764	arg1	ornithine					766:774	ornithine	766:774	the amino acids ornithine	750:774	The cell-wall peptidoglycan contained the amino acids ornithine, glutamic acid, glycine and alanine, the diagnostic diamino acid was ornithine.
33480836	5	45	dep	Microbacterium	562:575	arg1	suaedae					577:583	suaedae	577:583	suaedae	577:583	Phylogenetic analyses of the 16S rRNA gene sequences placed strain TRM 80801T within the genus Microbacterium with the highest similarities to Microbacterium suaedae YZYP 306T (98.97 %) and Microbacterium indicum BBH6T (98.17 %), respectively.
33480836	10	46	theme	fatty	997:1001	arg1	anteiso-C15 					1014:1025	anteiso-C15 	1014:1025	anteiso-C15 	1014:1025	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
33480836	10	46	theme	fatty	997:1001	arg1	acids					1003:1007	The major cellular fatty acids	978:1007	The major cellular fatty acids	978:1007	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
33480836	7	47	contain	contained	740:748	arg1	peptidoglycan					726:738	The cell-wall peptidoglycan	712:738	The cell-wall peptidoglycan	712:738	The cell-wall peptidoglycan contained the amino acids ornithine, glutamic acid, glycine and alanine, the diagnostic diamino acid was ornithine.
33480836	7	47	contain	contained	740:748	arg2	acids					760:764	the amino acids ornithine	750:774	the amino acids ornithine	750:774	The cell-wall peptidoglycan contained the amino acids ornithine, glutamic acid, glycine and alanine, the diagnostic diamino acid was ornithine.
33480836	7	47	contain	contained	740:748	arg2	glycine					792:798	glycine	792:798	glycine	792:798	The cell-wall peptidoglycan contained the amino acids ornithine, glutamic acid, glycine and alanine, the diagnostic diamino acid was ornithine.
33480836	7	47	contain	contained	740:748	arg2	alanine					804:810	alanine	804:810	alanine	804:810	The cell-wall peptidoglycan contained the amino acids ornithine, glutamic acid, glycine and alanine, the diagnostic diamino acid was ornithine.
33480836	7	47	contain	contained	740:748	arg2	acid					786:789	glutamic acid	777:789	glutamic acid	777:789	The cell-wall peptidoglycan contained the amino acids ornithine, glutamic acid, glycine and alanine, the diagnostic diamino acid was ornithine.
33480836	14	48	theme	novel	1416:1420	arg1	species					1422:1428	a novel species	1414:1428	a novel species	1414:1428	On the basis of the evidence presented in this study, strain TRM 80801T is representative of a novel species in the genus Microbacterium, for which the name Microbacterium karelineae sp.
33480836	7	49	theme	cell-wall	716:724	arg1	peptidoglycan					726:738	The cell-wall peptidoglycan	712:738	The cell-wall peptidoglycan	712:738	The cell-wall peptidoglycan contained the amino acids ornithine, glutamic acid, glycine and alanine, the diagnostic diamino acid was ornithine.
33480836	2	50	attach	isolated	140:147	arg1	Karelinea					158:166	the Karelinea	154:166	the Karelinea in Taklamakan desert, Xinjiang Uygur Autonomous Region, north-west China	154:239	A bacterial strain, designated TRM 80801T, was isolated from the Karelinea in Taklamakan desert, Xinjiang Uygur Autonomous Region, north-west China.
33480836	2	50	attach	isolated	140:147	arg2	strain					105:110	A bacterial strain	93:110	A bacterial strain	93:110	A bacterial strain, designated TRM 80801T, was isolated from the Karelinea in Taklamakan desert, Xinjiang Uygur Autonomous Region, north-west China.
33480836	4	51	theme	%	405:405	arg1	NaCl					413:416	1-15 % (w/v) NaCl	400:416	1-15 % (w/v) NaCl	400:416	Strain TRM 80801T grew at 4-50 °C, with optimum growth at 28 °C, and grew at pH 6.0-11.0 and 1-15 % (w/v) NaCl.
33480836	2	52	theme	Autonomous	205:214	arg1	Region					216:221	Autonomous Region	205:221	Autonomous Region	205:221	A bacterial strain, designated TRM 80801T, was isolated from the Karelinea in Taklamakan desert, Xinjiang Uygur Autonomous Region, north-west China.
33480836	5	53	theme	sequences	462:470	arg1	analyses					432:439	Phylogenetic analyses	419:439	Phylogenetic analyses of the 16S rRNA gene sequences	419:470	Phylogenetic analyses of the 16S rRNA gene sequences placed strain TRM 80801T within the genus Microbacterium with the highest similarities to Microbacterium suaedae YZYP 306T (98.97 %) and Microbacterium indicum BBH6T (98.17 %), respectively.
33480836	12	54	theme	polar	1126:1130	arg1	diphosphatidylglycerol					1144:1165	diphosphatidylglycerol	1144:1165	diphosphatidylglycerol	1144:1165	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol.
33480836	12	54	theme	polar	1126:1130	arg1	lipids					1132:1137	The polar lipids	1122:1137	The polar lipids	1122:1137	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol.
33480836	4	55	theme	1-15 	400:404	arg1	%					405:405	%	405:405	%	405:405	Strain TRM 80801T grew at 4-50 °C, with optimum growth at 28 °C, and grew at pH 6.0-11.0 and 1-15 % (w/v) NaCl.
33480836	4	56	theme	TRM	314:316	arg1	80801T					318:323	Strain TRM 80801T	307:323	Strain TRM 80801T	307:323	Strain TRM 80801T grew at 4-50 °C, with optimum growth at 28 °C, and grew at pH 6.0-11.0 and 1-15 % (w/v) NaCl.
33480836	14	57	theme	Microbacterium	1478:1491	arg1	sp					1504:1505	the name Microbacterium karelineae sp	1469:1505	the name Microbacterium karelineae sp	1469:1505	On the basis of the evidence presented in this study, strain TRM 80801T is representative of a novel species in the genus Microbacterium, for which the name Microbacterium karelineae sp.
33480836	5	58	with	Microbacterium	514:527	arg1	similarities					546:557	the highest similarities	534:557	the highest similarities to Microbacterium suaedae YZYP 306T (98.97 %) and Microbacterium indicum BBH6T (98.17 %), respectively	534:660	Phylogenetic analyses of the 16S rRNA gene sequences placed strain TRM 80801T within the genus Microbacterium with the highest similarities to Microbacterium suaedae YZYP 306T (98.97 %) and Microbacterium indicum BBH6T (98.17 %), respectively.
33480836	16	59	theme	TRM	1545:1547	arg1	49357T					1581:1586	=CCTCC AB 2019248T=KCTC 49357T	1557:1586	=CCTCC AB 2019248T=KCTC 49357T	1557:1586	The type strain is TRM 80801T (=CCTCC AB 2019248T=KCTC 49357T).
33480836	16	59	theme	TRM	1545:1547	arg1	strain					1535:1540	The type strain	1526:1540	The type strain	1526:1540	The type strain is TRM 80801T (=CCTCC AB 2019248T=KCTC 49357T).
33480836	16	59	theme	TRM	1545:1547	arg1	80801T					1549:1554	TRM 80801T	1545:1554	TRM 80801T (=CCTCC AB 2019248T=KCTC 49357T)	1545:1587	The type strain is TRM 80801T (=CCTCC AB 2019248T=KCTC 49357T).
33480836	5	60	theme	strain	479:484	arg1	80801T					490:495	strain TRM 80801T	479:495	strain TRM 80801T	479:495	Phylogenetic analyses of the 16S rRNA gene sequences placed strain TRM 80801T within the genus Microbacterium with the highest similarities to Microbacterium suaedae YZYP 306T (98.97 %) and Microbacterium indicum BBH6T (98.17 %), respectively.
33480836	14	61	theme	genus	1437:1441	arg1	Microbacterium					1443:1456	the genus Microbacterium	1433:1456	the genus Microbacterium	1433:1456	On the basis of the evidence presented in this study, strain TRM 80801T is representative of a novel species in the genus Microbacterium, for which the name Microbacterium karelineae sp.
33480836	9	62	theme	Whole-cell	905:914	arg1	sugars					916:921	Whole-cell sugars	905:921	Whole-cell sugars	905:921	Whole-cell sugars were ribose, mannose, glucose, rhamnose and galactose.
33480836	7	63	theme	diagnostic	817:826	arg1	ornithine					845:853	ornithine	845:853	ornithine	845:853	The cell-wall peptidoglycan contained the amino acids ornithine, glutamic acid, glycine and alanine, the diagnostic diamino acid was ornithine.
33480836	7	63	theme	diagnostic	817:826	arg1	acid					836:839	the diagnostic diamino acid	813:839	the diagnostic diamino acid	813:839	The cell-wall peptidoglycan contained the amino acids ornithine, glutamic acid, glycine and alanine, the diagnostic diamino acid was ornithine.
33480836	7	64	theme	diamino	828:834	arg1	ornithine					845:853	ornithine	845:853	ornithine	845:853	The cell-wall peptidoglycan contained the amino acids ornithine, glutamic acid, glycine and alanine, the diagnostic diamino acid was ornithine.
33480836	7	64	theme	diamino	828:834	arg1	acid					836:839	the diagnostic diamino acid	813:839	the diagnostic diamino acid	813:839	The cell-wall peptidoglycan contained the amino acids ornithine, glutamic acid, glycine and alanine, the diagnostic diamino acid was ornithine.
33480836	5	65	theme	TRM	486:488	arg1	80801T					490:495	strain TRM 80801T	479:495	strain TRM 80801T	479:495	Phylogenetic analyses of the 16S rRNA gene sequences placed strain TRM 80801T within the genus Microbacterium with the highest similarities to Microbacterium suaedae YZYP 306T (98.97 %) and Microbacterium indicum BBH6T (98.17 %), respectively.
33480836	10	66	theme	major	982:986	arg1	anteiso-C15 					1014:1025	anteiso-C15 	1014:1025	anteiso-C15 	1014:1025	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
33480836	10	66	theme	major	982:986	arg1	acids					1003:1007	The major cellular fatty acids	978:1007	The major cellular fatty acids	978:1007	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
33480836	5	67	theme	rRNA	452:455	arg1	sequences					462:470	the 16S rRNA gene sequences	444:470	the 16S rRNA gene sequences	444:470	Phylogenetic analyses of the 16S rRNA gene sequences placed strain TRM 80801T within the genus Microbacterium with the highest similarities to Microbacterium suaedae YZYP 306T (98.97 %) and Microbacterium indicum BBH6T (98.17 %), respectively.
33480836	13	68	theme	nucleotide	1215:1224	arg1	identity					1226:1233	average nucleotide identity	1207:1233	The whole-genome average nucleotide identity (ANI) value between strain TRM 80801T and Microbacterium suaedae YZYP 306T	1190:1308	The whole-genome average nucleotide identity (ANI) value between strain TRM 80801T and Microbacterium suaedae YZYP 306T is 70.2 %.
33480836	13	68	theme	nucleotide	1215:1224	arg1	ANI					1236:1238	ANI	1236:1238	ANI	1236:1238	The whole-genome average nucleotide identity (ANI) value between strain TRM 80801T and Microbacterium suaedae YZYP 306T is 70.2 %.
33480836	5	69	theme	Microbacterium	609:622	arg1	BBH6T					632:636	Microbacterium indicum BBH6T	609:636	Microbacterium indicum BBH6T (98.17 %)	609:646	Phylogenetic analyses of the 16S rRNA gene sequences placed strain TRM 80801T within the genus Microbacterium with the highest similarities to Microbacterium suaedae YZYP 306T (98.97 %) and Microbacterium indicum BBH6T (98.17 %), respectively.
33480836	5	69	theme	Microbacterium	609:622	arg1	%					645:645	98.17 %	639:645	98.17 %	639:645	Phylogenetic analyses of the 16S rRNA gene sequences placed strain TRM 80801T within the genus Microbacterium with the highest similarities to Microbacterium suaedae YZYP 306T (98.97 %) and Microbacterium indicum BBH6T (98.17 %), respectively.
33480836	6	70	theme	G+C	671:673	arg1	content					675:681	The DNA G+C content	663:681	The DNA G+C content of TRM 80801T	663:695	The DNA G+C content of TRM 80801T is 69.38 mol%.
33480836	6	70	theme	G+C	671:673	arg1	%					709:709	69.38 mol%	700:709	69.38 mol%	700:709	The DNA G+C content of TRM 80801T is 69.38 mol%.
33480836	13	71	theme	identity	1226:1233	arg1	%					1318:1318	70.2 %	1313:1318	70.2 %	1313:1318	The whole-genome average nucleotide identity (ANI) value between strain TRM 80801T and Microbacterium suaedae YZYP 306T is 70.2 %.
33480836	13	71	theme	identity	1226:1233	arg1	value					1241:1245	The whole-genome average nucleotide identity (ANI) value	1190:1245	The whole-genome average nucleotide identity (ANI) value between strain TRM 80801T and Microbacterium suaedae YZYP 306T	1190:1308	The whole-genome average nucleotide identity (ANI) value between strain TRM 80801T and Microbacterium suaedae YZYP 306T is 70.2 %.
33480836	7	72	theme	glutamic	777:784	arg1	acid					786:789	glutamic acid	777:789	glutamic acid	777:789	The cell-wall peptidoglycan contained the amino acids ornithine, glutamic acid, glycine and alanine, the diagnostic diamino acid was ornithine.
33480836	7	72	theme	glutamic	777:784	arg1	acids					760:764	the amino acids ornithine	750:774	the amino acids ornithine	750:774	The cell-wall peptidoglycan contained the amino acids ornithine, glutamic acid, glycine and alanine, the diagnostic diamino acid was ornithine.
33480836	5	73	theme	16S	448:450	arg1	sequences					462:470	the 16S rRNA gene sequences	444:470	the 16S rRNA gene sequences	444:470	Phylogenetic analyses of the 16S rRNA gene sequences placed strain TRM 80801T within the genus Microbacterium with the highest similarities to Microbacterium suaedae YZYP 306T (98.97 %) and Microbacterium indicum BBH6T (98.17 %), respectively.
33480836	14	74	theme	evidence	1341:1348	arg1	representative					1396:1409	representative	1396:1409	representative	1396:1409	On the basis of the evidence presented in this study, strain TRM 80801T is representative of a novel species in the genus Microbacterium, for which the name Microbacterium karelineae sp.
33480836	16	75	theme	=CCTCC AB	1557:1565	arg1	49357T					1581:1586	=CCTCC AB 2019248T=KCTC 49357T	1557:1586	=CCTCC AB 2019248T=KCTC 49357T	1557:1586	The type strain is TRM 80801T (=CCTCC AB 2019248T=KCTC 49357T).
33480836	16	75	theme	=CCTCC AB	1557:1565	arg1	80801T					1549:1554	TRM 80801T	1545:1554	TRM 80801T (=CCTCC AB 2019248T=KCTC 49357T)	1545:1587	The type strain is TRM 80801T (=CCTCC AB 2019248T=KCTC 49357T).
33480836	14	76	from	species	1422:1428	arg1	Microbacterium					1443:1456	the genus Microbacterium	1433:1456	the genus Microbacterium	1433:1456	On the basis of the evidence presented in this study, strain TRM 80801T is representative of a novel species in the genus Microbacterium, for which the name Microbacterium karelineae sp.
33480836	4	77	theme	Strain	307:312	arg1	80801T					318:323	Strain TRM 80801T	307:323	Strain TRM 80801T	307:323	Strain TRM 80801T grew at 4-50 °C, with optimum growth at 28 °C, and grew at pH 6.0-11.0 and 1-15 % (w/v) NaCl.
33480836	13	78	theme	YZYP	1300:1303	arg1	306T					1305:1308	Microbacterium suaedae YZYP 306T	1277:1308	Microbacterium suaedae YZYP 306T	1277:1308	The whole-genome average nucleotide identity (ANI) value between strain TRM 80801T and Microbacterium suaedae YZYP 306T is 70.2 %.
33480836	13	79	theme	strain	1255:1260	arg1	80801T					1266:1271	strain TRM 80801T	1255:1271	strain TRM 80801T	1255:1271	The whole-genome average nucleotide identity (ANI) value between strain TRM 80801T and Microbacterium suaedae YZYP 306T is 70.2 %.
34337208	8	0	theme	chemical	1257:1264	arg1	composition					1266:1276	composition	1266:1276	composition	1266:1276	Further, the chemical composition, morphology, and dispersibility of an array of mica pigment additives are characterized in detail as they pertain to the quality of an art medium.
34337208	6	1	theme	mica	877:880	arg1	pigments					882:889	mica pigments	877:889	mica pigments suspended in alginate as a new, vibrant art medium for 2D and 3D compositions	877:967	Here, we detail the formulation of a bioink consisting of mica pigments suspended in alginate as a new, vibrant art medium for 2D and 3D compositions.
34337208	6	2	theme	2D	946:947	arg1	compositions					956:967	2D and 3D compositions	946:967	2D and 3D compositions	946:967	Here, we detail the formulation of a bioink consisting of mica pigments suspended in alginate as a new, vibrant art medium for 2D and 3D compositions.
34337208	0	3	theme	Two-Dimensional	64:78	arg1	Art					102:104	Two-Dimensional and Three-Dimensional Art	64:104	Two-Dimensional and Three-Dimensional Art	64:104	Pearlescent Mica-Doped Alginate as a Stable, Vibrant Medium for Two-Dimensional and Three-Dimensional Art.
34337208	5	4	theme	derived	635:641	arg1	polymers					643:650	biologically derived polymers	622:650	biologically derived polymers such as polysaccharides used to create "bioinks"	622:699	In contrast, biologically derived polymers such as polysaccharides used to create "bioinks" often do not require heating the material for workability, broadening the types of techniques available for printing.
34337208	5	4	theme	derived	635:641	arg1	polysaccharides					660:674	polysaccharides	660:674	polysaccharides used to create "bioinks"	660:699	In contrast, biologically derived polymers such as polysaccharides used to create "bioinks" often do not require heating the material for workability, broadening the types of techniques available for printing.
34337208	3	5	theme	high	494:497	arg1	heat					499:502	high heat	494:502	high heat	494:502	Additively manufactured art relies heavily on plastic-based materials, which typically require high heat to induce melting for workability.
34337208	5	6	dep	used	676:679	arg1	polysaccharides					660:674	polysaccharides	660:674	polysaccharides used to create "bioinks"	660:699	In contrast, biologically derived polymers such as polysaccharides used to create "bioinks" often do not require heating the material for workability, broadening the types of techniques available for printing.
34337208	5	6	dep	used	676:679	arg1	create					684:689	create	684:689	used to create "bioinks"	676:699	In contrast, biologically derived polymers such as polysaccharides used to create "bioinks" often do not require heating the material for workability, broadening the types of techniques available for printing.
34337208	7	7	theme	low	1043:1045	arg1	cost					1047:1050	low cost	1043:1050	low cost with wide availability, nontoxicity and biocompatibility, minimal color, and an array of attractive physicochemical properties that offer workability and processing into 2D and 3D structures	1043:1241	The properties that make alginate an ideal colorant binder are detailed: low cost with wide availability, nontoxicity and biocompatibility, minimal color, and an array of attractive physicochemical properties that offer workability and processing into 2D and 3D structures.
34337208	8	8	theme	additives	1338:1346	arg1	array					1316:1320	an array	1313:1320	an array of mica pigment additives	1313:1346	Further, the chemical composition, morphology, and dispersibility of an array of mica pigment additives are characterized in detail as they pertain to the quality of an art medium.
34337208	10	9	theme	media	1681:1685	arg1	utility					1666:1672	The utility	1662:1672	The utility of the media	1662:1685	The utility of the media is demonstrated via the generation of 2D and 3D vibrant structures.
34337208	0	10	theme	Three-Dimensional	84:100	arg1	Art					102:104	Two-Dimensional and Three-Dimensional Art	64:104	Two-Dimensional and Three-Dimensional Art	64:104	Pearlescent Mica-Doped Alginate as a Stable, Vibrant Medium for Two-Dimensional and Three-Dimensional Art.
34337208	6	11	theme	bioink	856:861	arg1	formulation					839:849	the formulation	835:849	the formulation of a bioink consisting of mica pigments suspended in alginate as a new, vibrant art medium for 2D and 3D compositions	835:967	Here, we detail the formulation of a bioink consisting of mica pigments suspended in alginate as a new, vibrant art medium for 2D and 3D compositions.
34337208	9	12	theme	substrates	1584:1593	arg1	coverage					1572:1579	coverage	1572:1579	coverage	1572:1579	Alginate-based media with eight mica colors were formulated, where mica addition resulted in vibrantly colored inks with moderate hiding power and coverage of substrates necessary for 2D printing with thin horizontal and vertical lines.
34337208	9	12	theme	substrates	1584:1593	arg1	power					1562:1566	power	1562:1566	power	1562:1566	Alginate-based media with eight mica colors were formulated, where mica addition resulted in vibrantly colored inks with moderate hiding power and coverage of substrates necessary for 2D printing with thin horizontal and vertical lines.
34337208	10	13	theme	vibrant	1735:1741	arg1	structures					1743:1752	2D and 3D vibrant structures	1725:1752	2D and 3D vibrant structures	1725:1752	The utility of the media is demonstrated via the generation of 2D and 3D vibrant structures.
34337208	4	14	theme	plastic	569:575	arg1	media					577:581	plastic media	569:581	plastic media	569:581	The necessity for heat limits plastic media to dedicated 3D printers.
34337208	9	15	theme	necessary	1595:1603	arg1	power					1562:1566	power	1562:1566	power	1562:1566	Alginate-based media with eight mica colors were formulated, where mica addition resulted in vibrantly colored inks with moderate hiding power and coverage of substrates necessary for 2D printing with thin horizontal and vertical lines.
34337208	1	16	theme	media	185:189	arg1	development					155:165	the development	151:165	the development of innovative art media that progress the field of modern art	151:227	Emergent technologies are driving forces in the development of innovative art media that progress the field of modern art.
34337208	1	17	theme	innovative	170:179	arg1	media					185:189	innovative art media	170:189	innovative art media that progress the field of modern art	170:227	Emergent technologies are driving forces in the development of innovative art media that progress the field of modern art.
34337208	6	18	theme	art	931:933	arg1	medium					935:940	a new, vibrant art medium	916:940	a new, vibrant art medium for 2D and 3D compositions	916:967	Here, we detail the formulation of a bioink consisting of mica pigments suspended in alginate as a new, vibrant art medium for 2D and 3D compositions.
34337208	7	19	dep	detailed	1033:1040	arg1	cost					1047:1050	low cost	1043:1050	low cost with wide availability, nontoxicity and biocompatibility, minimal color, and an array of attractive physicochemical properties that offer workability and processing into 2D and 3D structures	1043:1241	The properties that make alginate an ideal colorant binder are detailed: low cost with wide availability, nontoxicity and biocompatibility, minimal color, and an array of attractive physicochemical properties that offer workability and processing into 2D and 3D structures.
34337208	1	20	theme	art	181:183	arg1	media					185:189	innovative art media	170:189	innovative art media that progress the field of modern art	170:227	Emergent technologies are driving forces in the development of innovative art media that progress the field of modern art.
34337208	0	21	theme	Mica-Doped	12:21	arg1	Alginate					23:30	Mica-Doped Alginate	12:30	Mica-Doped Alginate	12:30	Pearlescent Mica-Doped Alginate as a Stable, Vibrant Medium for Two-Dimensional and Three-Dimensional Art.
34337208	0	21	theme	Mica-Doped	12:21	arg1	Medium					53:58	a Stable, Vibrant Medium	35:58	a Stable, Vibrant Medium for Two-Dimensional and Three-Dimensional Art	35:104	Pearlescent Mica-Doped Alginate as a Stable, Vibrant Medium for Two-Dimensional and Three-Dimensional Art.
34337208	7	22	theme	attractive	1141:1150	arg1	properties					1168:1177	attractive physicochemical properties	1141:1177	attractive physicochemical properties that offer workability and processing into 2D and 3D structures	1141:1241	The properties that make alginate an ideal colorant binder are detailed: low cost with wide availability, nontoxicity and biocompatibility, minimal color, and an array of attractive physicochemical properties that offer workability and processing into 2D and 3D structures.
34337208	9	23	theme	2D	1609:1610	arg1	printing					1612:1619	2D printing	1609:1619	2D printing	1609:1619	Alginate-based media with eight mica colors were formulated, where mica addition resulted in vibrantly colored inks with moderate hiding power and coverage of substrates necessary for 2D printing with thin horizontal and vertical lines.
34337208	1	24	from	forces	141:146	arg1	development					155:165	the development	151:165	the development of innovative art media that progress the field of modern art	151:227	Emergent technologies are driving forces in the development of innovative art media that progress the field of modern art.
34337208	7	25	theme	minimal	1110:1116	arg1	color					1118:1122	minimal color	1110:1122	minimal color	1110:1122	The properties that make alginate an ideal colorant binder are detailed: low cost with wide availability, nontoxicity and biocompatibility, minimal color, and an array of attractive physicochemical properties that offer workability and processing into 2D and 3D structures.
34337208	2	26	dep	capitalized	253:263	arg1	manufacturing					349:361	additive manufacturing	340:361	additive manufacturing	340:361	Recently, artists have capitalized on the versatility of a new technology to create, restore, and modify art: additive manufacturing or three-dimensional (3D) printing.
34337208	2	26	dep	capitalized	253:263	arg1	printing					389:396	three-dimensional (3D) printing	366:396	three-dimensional (3D) printing	366:396	Recently, artists have capitalized on the versatility of a new technology to create, restore, and modify art: additive manufacturing or three-dimensional (3D) printing.
34337208	9	27	theme	horizontal	1631:1640	arg1	lines					1655:1659	thin horizontal and vertical lines	1626:1659	thin horizontal and vertical lines	1626:1659	Alginate-based media with eight mica colors were formulated, where mica addition resulted in vibrantly colored inks with moderate hiding power and coverage of substrates necessary for 2D printing with thin horizontal and vertical lines.
34337208	2	28	theme	technology	293:302	arg1	versatility					272:282	the versatility	268:282	the versatility of a new technology to create, restore, and modify art	268:337	Recently, artists have capitalized on the versatility of a new technology to create, restore, and modify art: additive manufacturing or three-dimensional (3D) printing.
34337208	2	29	theme	additive	340:347	arg1	manufacturing					349:361	additive manufacturing	340:361	additive manufacturing	340:361	Recently, artists have capitalized on the versatility of a new technology to create, restore, and modify art: additive manufacturing or three-dimensional (3D) printing.
34337208	5	30	theme	techniques	784:793	arg1	types					775:779	the types	771:779	the types of techniques available for printing	771:816	In contrast, biologically derived polymers such as polysaccharides used to create "bioinks" often do not require heating the material for workability, broadening the types of techniques available for printing.
34337208	7	31	dep	alginate	995:1002	arg1	binder					1022:1027	an ideal colorant binder	1004:1027	alginate an ideal colorant binder	995:1027	The properties that make alginate an ideal colorant binder are detailed: low cost with wide availability, nontoxicity and biocompatibility, minimal color, and an array of attractive physicochemical properties that offer workability and processing into 2D and 3D structures.
34337208	7	32	theme	ideal	1007:1011	arg1	binder					1022:1027	an ideal colorant binder	1004:1027	alginate an ideal colorant binder	995:1027	The properties that make alginate an ideal colorant binder are detailed: low cost with wide availability, nontoxicity and biocompatibility, minimal color, and an array of attractive physicochemical properties that offer workability and processing into 2D and 3D structures.
34337208	9	33	with	media	1440:1444	arg1	colors					1462:1467	eight mica colors	1451:1467	eight mica colors	1451:1467	Alginate-based media with eight mica colors were formulated, where mica addition resulted in vibrantly colored inks with moderate hiding power and coverage of substrates necessary for 2D printing with thin horizontal and vertical lines.
34337208	2	34	theme	new	289:291	arg1	technology					293:302	a new technology	287:302	a new technology to create, restore, and modify art	287:337	Recently, artists have capitalized on the versatility of a new technology to create, restore, and modify art: additive manufacturing or three-dimensional (3D) printing.
34337208	5	35	theme	available	795:803	arg1	techniques					784:793	techniques	784:793	techniques available for printing	784:816	In contrast, biologically derived polymers such as polysaccharides used to create "bioinks" often do not require heating the material for workability, broadening the types of techniques available for printing.
34337208	6	36	theme	new	918:920	arg1	medium					935:940	a new, vibrant art medium	916:940	a new, vibrant art medium for 2D and 3D compositions	916:967	Here, we detail the formulation of a bioink consisting of mica pigments suspended in alginate as a new, vibrant art medium for 2D and 3D compositions.
34337208	7	37	theme	colorant	1013:1020	arg1	binder					1022:1027	an ideal colorant binder	1004:1027	alginate an ideal colorant binder	995:1027	The properties that make alginate an ideal colorant binder are detailed: low cost with wide availability, nontoxicity and biocompatibility, minimal color, and an array of attractive physicochemical properties that offer workability and processing into 2D and 3D structures.
34337208	1	38	theme	Emergent	107:114	arg1	technologies					116:127	Emergent technologies	107:127	Emergent technologies	107:127	Emergent technologies are driving forces in the development of innovative art media that progress the field of modern art.
34337208	1	38	theme	Emergent	107:114	arg1	forces					141:146	driving forces	133:146	driving forces	133:146	Emergent technologies are driving forces in the development of innovative art media that progress the field of modern art.
34337208	9	39	theme	mica	1457:1460	arg1	colors					1462:1467	eight mica colors	1451:1467	eight mica colors	1451:1467	Alginate-based media with eight mica colors were formulated, where mica addition resulted in vibrantly colored inks with moderate hiding power and coverage of substrates necessary for 2D printing with thin horizontal and vertical lines.
34337208	3	40	theme	plastic-based	445:457	arg1	materials					459:467	plastic-based materials	445:467	plastic-based materials	445:467	Additively manufactured art relies heavily on plastic-based materials, which typically require high heat to induce melting for workability.
34337208	0	41	theme	Stable	37:42	arg1	Alginate					23:30	Mica-Doped Alginate	12:30	Mica-Doped Alginate	12:30	Pearlescent Mica-Doped Alginate as a Stable, Vibrant Medium for Two-Dimensional and Three-Dimensional Art.
34337208	0	41	theme	Stable	37:42	arg1	Medium					53:58	a Stable, Vibrant Medium	35:58	a Stable, Vibrant Medium for Two-Dimensional and Three-Dimensional Art	35:104	Pearlescent Mica-Doped Alginate as a Stable, Vibrant Medium for Two-Dimensional and Three-Dimensional Art.
34337208	5	42	theme	bioinks	692:698	arg1	"					699:699	"bioinks"	691:699	"bioinks"	691:699	In contrast, biologically derived polymers such as polysaccharides used to create "bioinks" often do not require heating the material for workability, broadening the types of techniques available for printing.
34337208	8	43	theme	mica	1325:1328	arg1	additives					1338:1346	mica pigment additives	1325:1346	mica pigment additives	1325:1346	Further, the chemical composition, morphology, and dispersibility of an array of mica pigment additives are characterized in detail as they pertain to the quality of an art medium.
34337208	7	44	theme	3D	1229:1230	arg1	structures					1232:1241	2D and 3D structures	1222:1241	2D and 3D structures	1222:1241	The properties that make alginate an ideal colorant binder are detailed: low cost with wide availability, nontoxicity and biocompatibility, minimal color, and an array of attractive physicochemical properties that offer workability and processing into 2D and 3D structures.
34337208	3	45	theme	manufactured	410:421	arg1	art					423:425	Additively manufactured art	399:425	Additively manufactured art	399:425	Additively manufactured art relies heavily on plastic-based materials, which typically require high heat to induce melting for workability.
34337208	10	46	theme	2D	1725:1726	arg1	structures					1743:1752	2D and 3D vibrant structures	1725:1752	2D and 3D vibrant structures	1725:1752	The utility of the media is demonstrated via the generation of 2D and 3D vibrant structures.
34337208	5	47	link	derived	635:641	arg1	polymers					643:650	biologically derived polymers	622:650	biologically derived polymers such as polysaccharides used to create "bioinks"	622:699	In contrast, biologically derived polymers such as polysaccharides used to create "bioinks" often do not require heating the material for workability, broadening the types of techniques available for printing.
34337208	5	47	link	derived	635:641	arg1	polysaccharides					660:674	polysaccharides	660:674	polysaccharides used to create "bioinks"	660:699	In contrast, biologically derived polymers such as polysaccharides used to create "bioinks" often do not require heating the material for workability, broadening the types of techniques available for printing.
34337208	7	48	theme	wide	1057:1060	arg1	availability					1062:1073	wide availability	1057:1073	wide availability	1057:1073	The properties that make alginate an ideal colorant binder are detailed: low cost with wide availability, nontoxicity and biocompatibility, minimal color, and an array of attractive physicochemical properties that offer workability and processing into 2D and 3D structures.
34337208	9	49	theme	vertical	1646:1653	arg1	lines					1655:1659	thin horizontal and vertical lines	1626:1659	thin horizontal and vertical lines	1626:1659	Alginate-based media with eight mica colors were formulated, where mica addition resulted in vibrantly colored inks with moderate hiding power and coverage of substrates necessary for 2D printing with thin horizontal and vertical lines.
34337208	0	50	dep	Stable	37:42	arg1	Vibrant					45:51	Vibrant	45:51	Vibrant	45:51	Pearlescent Mica-Doped Alginate as a Stable, Vibrant Medium for Two-Dimensional and Three-Dimensional Art.
34337208	7	51	theme	2D	1222:1223	arg1	structures					1232:1241	2D and 3D structures	1222:1241	2D and 3D structures	1222:1241	The properties that make alginate an ideal colorant binder are detailed: low cost with wide availability, nontoxicity and biocompatibility, minimal color, and an array of attractive physicochemical properties that offer workability and processing into 2D and 3D structures.
34337208	9	52	theme	Alginate-based	1425:1438	arg1	media					1440:1444	Alginate-based media	1425:1444	Alginate-based media with eight mica colors	1425:1467	Alginate-based media with eight mica colors were formulated, where mica addition resulted in vibrantly colored inks with moderate hiding power and coverage of substrates necessary for 2D printing with thin horizontal and vertical lines.
34337208	2	53	theme	three-dimensional	366:382	arg1	printing					389:396	three-dimensional (3D) printing	366:396	three-dimensional (3D) printing	366:396	Recently, artists have capitalized on the versatility of a new technology to create, restore, and modify art: additive manufacturing or three-dimensional (3D) printing.
34337208	6	54	dep	new	918:920	arg1	vibrant					923:929	vibrant	923:929	vibrant	923:929	Here, we detail the formulation of a bioink consisting of mica pigments suspended in alginate as a new, vibrant art medium for 2D and 3D compositions.
34337208	8	55	theme	pigment	1330:1336	arg1	additives					1338:1346	mica pigment additives	1325:1346	mica pigment additives	1325:1346	Further, the chemical composition, morphology, and dispersibility of an array of mica pigment additives are characterized in detail as they pertain to the quality of an art medium.
34337208	9	56	theme	mica	1492:1495	arg1	addition					1497:1504	mica addition	1492:1504	mica addition	1492:1504	Alginate-based media with eight mica colors were formulated, where mica addition resulted in vibrantly colored inks with moderate hiding power and coverage of substrates necessary for 2D printing with thin horizontal and vertical lines.
34337208	9	57	theme	colored	1528:1534	arg1	inks					1536:1539	vibrantly colored inks	1518:1539	vibrantly colored inks with moderate	1518:1553	Alginate-based media with eight mica colors were formulated, where mica addition resulted in vibrantly colored inks with moderate hiding power and coverage of substrates necessary for 2D printing with thin horizontal and vertical lines.
34337208	1	58	theme	driving	133:139	arg1	technologies					116:127	Emergent technologies	107:127	Emergent technologies	107:127	Emergent technologies are driving forces in the development of innovative art media that progress the field of modern art.
34337208	1	58	theme	driving	133:139	arg1	forces					141:146	driving forces	133:146	driving forces	133:146	Emergent technologies are driving forces in the development of innovative art media that progress the field of modern art.
34337208	8	59	theme	array	1316:1320	arg1	morphology					1279:1288	morphology	1279:1288	morphology	1279:1288	Further, the chemical composition, morphology, and dispersibility of an array of mica pigment additives are characterized in detail as they pertain to the quality of an art medium.
34337208	8	59	theme	array	1316:1320	arg1	dispersibility					1295:1308	dispersibility	1295:1308	dispersibility	1295:1308	Further, the chemical composition, morphology, and dispersibility of an array of mica pigment additives are characterized in detail as they pertain to the quality of an art medium.
34337208	8	59	theme	array	1316:1320	arg1	composition					1266:1276	composition	1266:1276	composition	1266:1276	Further, the chemical composition, morphology, and dispersibility of an array of mica pigment additives are characterized in detail as they pertain to the quality of an art medium.
34337208	1	60	theme	modern	218:223	arg1	art					225:227	modern art	218:227	modern art	218:227	Emergent technologies are driving forces in the development of innovative art media that progress the field of modern art.
34337208	9	61	with	inks	1536:1539	arg1	moderate					1546:1553	moderate	1546:1553	moderate	1546:1553	Alginate-based media with eight mica colors were formulated, where mica addition resulted in vibrantly colored inks with moderate hiding power and coverage of substrates necessary for 2D printing with thin horizontal and vertical lines.
34337208	10	62	theme	structures	1743:1752	arg1	generation					1711:1720	the generation	1707:1720	the generation of 2D and 3D vibrant structures	1707:1752	The utility of the media is demonstrated via the generation of 2D and 3D vibrant structures.
34337208	8	63	theme	art	1413:1415	arg1	medium					1417:1422	an art medium	1410:1422	an art medium	1410:1422	Further, the chemical composition, morphology, and dispersibility of an array of mica pigment additives are characterized in detail as they pertain to the quality of an art medium.
34337208	7	64	with	cost	1047:1050	arg1	nontoxicity					1076:1086	nontoxicity	1076:1086	nontoxicity	1076:1086	The properties that make alginate an ideal colorant binder are detailed: low cost with wide availability, nontoxicity and biocompatibility, minimal color, and an array of attractive physicochemical properties that offer workability and processing into 2D and 3D structures.
34337208	7	64	with	cost	1047:1050	arg1	availability					1062:1073	wide availability	1057:1073	wide availability	1057:1073	The properties that make alginate an ideal colorant binder are detailed: low cost with wide availability, nontoxicity and biocompatibility, minimal color, and an array of attractive physicochemical properties that offer workability and processing into 2D and 3D structures.
34337208	7	64	with	cost	1047:1050	arg1	array					1132:1136	an array	1129:1136	an array of attractive physicochemical properties that offer workability and processing into 2D and 3D structures	1129:1241	The properties that make alginate an ideal colorant binder are detailed: low cost with wide availability, nontoxicity and biocompatibility, minimal color, and an array of attractive physicochemical properties that offer workability and processing into 2D and 3D structures.
34337208	7	64	with	cost	1047:1050	arg1	color					1118:1122	minimal color	1110:1122	minimal color	1110:1122	The properties that make alginate an ideal colorant binder are detailed: low cost with wide availability, nontoxicity and biocompatibility, minimal color, and an array of attractive physicochemical properties that offer workability and processing into 2D and 3D structures.
34337208	1	65	theme	art	225:227	arg1	field					209:213	the field	205:213	the field of modern art	205:227	Emergent technologies are driving forces in the development of innovative art media that progress the field of modern art.
34337208	7	66	theme	physicochemical	1152:1166	arg1	properties					1168:1177	attractive physicochemical properties	1141:1177	attractive physicochemical properties that offer workability and processing into 2D and 3D structures	1141:1241	The properties that make alginate an ideal colorant binder are detailed: low cost with wide availability, nontoxicity and biocompatibility, minimal color, and an array of attractive physicochemical properties that offer workability and processing into 2D and 3D structures.
34337208	2	67	dep	three-dimensional	366:382	arg1	3D					385:386	3D	385:386	3D	385:386	Recently, artists have capitalized on the versatility of a new technology to create, restore, and modify art: additive manufacturing or three-dimensional (3D) printing.
34337208	6	68	theme	3D	953:954	arg1	compositions					956:967	2D and 3D compositions	946:967	2D and 3D compositions	946:967	Here, we detail the formulation of a bioink consisting of mica pigments suspended in alginate as a new, vibrant art medium for 2D and 3D compositions.
34337208	7	69	theme	properties	1168:1177	arg1	nontoxicity					1076:1086	nontoxicity	1076:1086	nontoxicity	1076:1086	The properties that make alginate an ideal colorant binder are detailed: low cost with wide availability, nontoxicity and biocompatibility, minimal color, and an array of attractive physicochemical properties that offer workability and processing into 2D and 3D structures.
34337208	7	69	theme	properties	1168:1177	arg1	availability					1062:1073	wide availability	1057:1073	wide availability	1057:1073	The properties that make alginate an ideal colorant binder are detailed: low cost with wide availability, nontoxicity and biocompatibility, minimal color, and an array of attractive physicochemical properties that offer workability and processing into 2D and 3D structures.
34337208	7	69	theme	properties	1168:1177	arg1	array					1132:1136	an array	1129:1136	an array of attractive physicochemical properties that offer workability and processing into 2D and 3D structures	1129:1241	The properties that make alginate an ideal colorant binder are detailed: low cost with wide availability, nontoxicity and biocompatibility, minimal color, and an array of attractive physicochemical properties that offer workability and processing into 2D and 3D structures.
34337208	7	69	theme	properties	1168:1177	arg1	biocompatibility					1092:1107	biocompatibility	1092:1107	biocompatibility	1092:1107	The properties that make alginate an ideal colorant binder are detailed: low cost with wide availability, nontoxicity and biocompatibility, minimal color, and an array of attractive physicochemical properties that offer workability and processing into 2D and 3D structures.
34337208	7	69	theme	properties	1168:1177	arg1	color					1118:1122	minimal color	1110:1122	minimal color	1110:1122	The properties that make alginate an ideal colorant binder are detailed: low cost with wide availability, nontoxicity and biocompatibility, minimal color, and an array of attractive physicochemical properties that offer workability and processing into 2D and 3D structures.
34337208	8	70	theme	medium	1417:1422	arg1	quality					1399:1405	the quality	1395:1405	the quality of an art medium	1395:1422	Further, the chemical composition, morphology, and dispersibility of an array of mica pigment additives are characterized in detail as they pertain to the quality of an art medium.
34337208	10	71	theme	3D	1732:1733	arg1	structures					1743:1752	2D and 3D vibrant structures	1725:1752	2D and 3D vibrant structures	1725:1752	The utility of the media is demonstrated via the generation of 2D and 3D vibrant structures.
34337208	4	72	theme	dedicated	586:594	arg1	printers					599:606	dedicated 3D printers	586:606	dedicated 3D printers	586:606	The necessity for heat limits plastic media to dedicated 3D printers.
34337208	9	73	theme	thin	1626:1629	arg1	lines					1655:1659	thin horizontal and vertical lines	1626:1659	thin horizontal and vertical lines	1626:1659	Alginate-based media with eight mica colors were formulated, where mica addition resulted in vibrantly colored inks with moderate hiding power and coverage of substrates necessary for 2D printing with thin horizontal and vertical lines.
34337208	4	74	theme	3D	596:597	arg1	printers					599:606	dedicated 3D printers	586:606	dedicated 3D printers	586:606	The necessity for heat limits plastic media to dedicated 3D printers.
33510716	4	0	dep	Pseudomonas	528:538	arg1	fluorescens					540:550	fluorescens	540:550	fluorescens	540:550	In this work, we monitor structural features of Pseudomonas fluorescens biofilms grown with and without hydrodynamic stress.
33510716	6	1	theme	growth	744:749	arg1	timescales					751:760	short growth timescales	738:760	short growth timescales	738:760	At short growth timescales, the matrix mediates a weak cell-cell attractive interaction due to the depletion forces originated by the polymer constituents.
33510716	2	2	theme	stimuli	351:357	arg1	presence					330:337	the presence	326:337	the presence of external stimuli such as hydrodynamic stress	326:385	It is known that bacteria change their colony/matrix ratio in the presence of external stimuli such as hydrodynamic stress.
33510716	4	3	theme	hydrodynamic	584:595	arg1	stress					597:602	hydrodynamic stress	584:602	hydrodynamic stress	584:602	In this work, we monitor structural features of Pseudomonas fluorescens biofilms grown with and without hydrodynamic stress.
33510716	8	4	theme	computer	1216:1223	arg1	simulations					1225:1235	computer simulations	1216:1235	computer simulations	1216:1235	This results in the formation of more mechanically stable biofilms due to an increase of the number of crosslinks, as shown by computer simulations.
33510716	4	5	theme	structural	505:514	arg1	features					516:523	structural features	505:523	structural features of Pseudomonas fluorescens biofilms grown with and without hydrodynamic stress	505:602	In this work, we monitor structural features of Pseudomonas fluorescens biofilms grown with and without hydrodynamic stress.
33510716	10	6	theme	hostile	1480:1486	arg1	stress					1501:1506	hostile hydrodynamic stress	1480:1506	hostile hydrodynamic stress	1480:1506	Overall, we demonstrate that bacteria are capable of self-adapting to hostile hydrodynamic stress by tailoring the biofilm chemical composition, thus affecting both the mesoscale structure of the matrix and its viscoelastic properties that ultimately regulate the bacteria-polymer interactions.
33510716	7	7	theme	nutrients	992:1000	arg1	diffusion					979:987	a faster diffusion	970:987	a faster diffusion of nutrients and a higher incorporation of planktonic bacteria to the already formed microcolonies	970:1086	Using a population dynamics model, we conclude that hydrodynamic stress causes a faster diffusion of nutrients and a higher incorporation of planktonic bacteria to the already formed microcolonies.
33510716	2	8	theme	external	342:349	arg1	stimuli					351:357	external stimuli	342:357	external stimuli such as hydrodynamic stress	342:385	It is known that bacteria change their colony/matrix ratio in the presence of external stimuli such as hydrodynamic stress.
33510716	2	8	theme	external	342:349	arg1	stress					380:385	hydrodynamic stress	367:385	hydrodynamic stress	367:385	It is known that bacteria change their colony/matrix ratio in the presence of external stimuli such as hydrodynamic stress.
33510716	7	9	theme	population	899:908	arg1	dynamics					910:917	population dynamics	899:917	a population dynamics model	897:923	Using a population dynamics model, we conclude that hydrodynamic stress causes a faster diffusion of nutrients and a higher incorporation of planktonic bacteria to the already formed microcolonies.
33510716	10	10	theme	matrix	1606:1611	arg1	properties					1634:1643	its viscoelastic properties	1617:1643	its viscoelastic properties that ultimately regulate the bacteria-polymer interactions	1617:1702	Overall, we demonstrate that bacteria are capable of self-adapting to hostile hydrodynamic stress by tailoring the biofilm chemical composition, thus affecting both the mesoscale structure of the matrix and its viscoelastic properties that ultimately regulate the bacteria-polymer interactions.
33510716	10	10	theme	matrix	1606:1611	arg1	structure					1589:1597	the mesoscale structure	1575:1597	the mesoscale structure of the matrix	1575:1611	Overall, we demonstrate that bacteria are capable of self-adapting to hostile hydrodynamic stress by tailoring the biofilm chemical composition, thus affecting both the mesoscale structure of the matrix and its viscoelastic properties that ultimately regulate the bacteria-polymer interactions.
33510716	10	11	theme	chemical	1533:1540	arg1	composition					1542:1552	the biofilm chemical composition	1521:1552	the biofilm chemical composition	1521:1552	Overall, we demonstrate that bacteria are capable of self-adapting to hostile hydrodynamic stress by tailoring the biofilm chemical composition, thus affecting both the mesoscale structure of the matrix and its viscoelastic properties that ultimately regulate the bacteria-polymer interactions.
33510716	5	12	theme	cell	684:687	arg1	population					697:706	the cell density population	680:706	the cell density population	680:706	Our measurements show that the hydrodynamic stress concomitantly increases the cell density population and the matrix production.
33510716	6	13	theme	depletion	834:842	arg1	forces					844:849	the depletion forces	830:849	the depletion forces originated by the polymer constituents	830:888	At short growth timescales, the matrix mediates a weak cell-cell attractive interaction due to the depletion forces originated by the polymer constituents.
33510716	7	14	theme	dynamics	910:917	arg1	model					919:923	a population dynamics model	897:923	a population dynamics model	897:923	Using a population dynamics model, we conclude that hydrodynamic stress causes a faster diffusion of nutrients and a higher incorporation of planktonic bacteria to the already formed microcolonies.
33510716	8	15	theme	number	1182:1187	arg1	increase					1166:1173	an increase	1163:1173	an increase of the number of crosslinks	1163:1201	This results in the formation of more mechanically stable biofilms due to an increase of the number of crosslinks, as shown by computer simulations.
33510716	9	16	theme	matrix	1323:1328	arg1	compositions					1303:1314	the chemical compositions	1290:1314	the chemical compositions	1290:1314	The mechanical stability also relies on a change in the chemical compositions of the matrix, which becomes enriched in carbohydrates, known to display adhering properties.
33510716	5	17	theme	density	689:695	arg1	population					697:706	the cell density population	680:706	the cell density population	680:706	Our measurements show that the hydrodynamic stress concomitantly increases the cell density population and the matrix production.
33510716	6	18	theme	short	738:742	arg1	timescales					751:760	short growth timescales	738:760	short growth timescales	738:760	At short growth timescales, the matrix mediates a weak cell-cell attractive interaction due to the depletion forces originated by the polymer constituents.
33510716	9	19	from	carbohydrates	1357:1369	arg1	enriched					1345:1352	enriched	1345:1352	enriched	1345:1352	The mechanical stability also relies on a change in the chemical compositions of the matrix, which becomes enriched in carbohydrates, known to display adhering properties.
33510716	1	20	theme	embedding	183:191	arg1	matrix					193:198	a self-produced embedding matrix	167:198	a self-produced embedding matrix	167:198	In some conditions, bacteria self-organize into biofilms, supracellular structures made of a self-produced embedding matrix, mainly composed of polysaccharides, DNA, proteins, and lipids.
33510716	8	21	theme	crosslinks	1192:1201	arg1	number					1182:1187	the number	1178:1187	the number of crosslinks	1178:1201	This results in the formation of more mechanically stable biofilms due to an increase of the number of crosslinks, as shown by computer simulations.
33510716	5	22	theme	hydrodynamic	636:647	arg1	stress					649:654	the hydrodynamic stress	632:654	the hydrodynamic stress	632:654	Our measurements show that the hydrodynamic stress concomitantly increases the cell density population and the matrix production.
33510716	6	23	theme	due	823:825	arg1	interaction					811:821	a weak cell-cell attractive interaction	783:821	a weak cell-cell attractive interaction due to the depletion forces originated by the polymer constituents	783:888	At short growth timescales, the matrix mediates a weak cell-cell attractive interaction due to the depletion forces originated by the polymer constituents.
33510716	3	24	theme	molecular	429:437	arg1	mechanisms					439:448	the molecular mechanisms	425:448	the molecular mechanisms driving this self-adaptation	425:477	However, little is still known about the molecular mechanisms driving this self-adaptation.
33510716	9	25	from	change	1280:1285	arg1	compositions					1303:1314	the chemical compositions	1290:1314	the chemical compositions	1290:1314	The mechanical stability also relies on a change in the chemical compositions of the matrix, which becomes enriched in carbohydrates, known to display adhering properties.
33510716	9	26	from	enriched	1345:1352	arg1	carbohydrates					1357:1369	carbohydrates	1357:1369	carbohydrates	1357:1369	The mechanical stability also relies on a change in the chemical compositions of the matrix, which becomes enriched in carbohydrates, known to display adhering properties.
33510716	7	27	theme	faster	972:977	arg1	diffusion					979:987	a faster diffusion	970:987	a faster diffusion of nutrients and a higher incorporation of planktonic bacteria to the already formed microcolonies	970:1086	Using a population dynamics model, we conclude that hydrodynamic stress causes a faster diffusion of nutrients and a higher incorporation of planktonic bacteria to the already formed microcolonies.
33510716	10	28	theme	viscoelastic	1621:1632	arg1	properties					1634:1643	its viscoelastic properties	1617:1643	its viscoelastic properties that ultimately regulate the bacteria-polymer interactions	1617:1702	Overall, we demonstrate that bacteria are capable of self-adapting to hostile hydrodynamic stress by tailoring the biofilm chemical composition, thus affecting both the mesoscale structure of the matrix and its viscoelastic properties that ultimately regulate the bacteria-polymer interactions.
33510716	6	29	theme	attractive	800:809	arg1	interaction					811:821	a weak cell-cell attractive interaction	783:821	a weak cell-cell attractive interaction due to the depletion forces originated by the polymer constituents	783:888	At short growth timescales, the matrix mediates a weak cell-cell attractive interaction due to the depletion forces originated by the polymer constituents.
33510716	2	30	theme	colony/matrix	303:315	arg1	ratio					317:321	their colony/matrix ratio	297:321	their colony/matrix ratio	297:321	It is known that bacteria change their colony/matrix ratio in the presence of external stimuli such as hydrodynamic stress.
33510716	2	31	theme	hydrodynamic	367:378	arg1	stress					380:385	hydrodynamic stress	367:385	hydrodynamic stress	367:385	It is known that bacteria change their colony/matrix ratio in the presence of external stimuli such as hydrodynamic stress.
33510716	0	32	theme	fluorescens	31:41	arg1	Self-Adaptation					0:14	Self-Adaptation	0:14	Self-Adaptation of Pseudomonas fluorescens	0:41	Self-Adaptation of Pseudomonas fluorescens Biofilms to Hydrodynamic Stress.
33510716	4	33	theme	biofilms	552:559	arg1	features					516:523	structural features	505:523	structural features of Pseudomonas fluorescens biofilms grown with and without hydrodynamic stress	505:602	In this work, we monitor structural features of Pseudomonas fluorescens biofilms grown with and without hydrodynamic stress.
33510716	10	34	theme	biofilm	1525:1531	arg1	composition					1542:1552	the biofilm chemical composition	1521:1552	the biofilm chemical composition	1521:1552	Overall, we demonstrate that bacteria are capable of self-adapting to hostile hydrodynamic stress by tailoring the biofilm chemical composition, thus affecting both the mesoscale structure of the matrix and its viscoelastic properties that ultimately regulate the bacteria-polymer interactions.
33510716	6	35	theme	cell-cell	790:798	arg1	interaction					811:821	a weak cell-cell attractive interaction	783:821	a weak cell-cell attractive interaction due to the depletion forces originated by the polymer constituents	783:888	At short growth timescales, the matrix mediates a weak cell-cell attractive interaction due to the depletion forces originated by the polymer constituents.
33510716	1	36	theme	self-produced	169:181	arg1	matrix					193:198	a self-produced embedding matrix	167:198	a self-produced embedding matrix	167:198	In some conditions, bacteria self-organize into biofilms, supracellular structures made of a self-produced embedding matrix, mainly composed of polysaccharides, DNA, proteins, and lipids.
33510716	10	37	theme	bacteria-polymer	1674:1689	arg1	interactions					1691:1702	the bacteria-polymer interactions	1670:1702	the bacteria-polymer interactions	1670:1702	Overall, we demonstrate that bacteria are capable of self-adapting to hostile hydrodynamic stress by tailoring the biofilm chemical composition, thus affecting both the mesoscale structure of the matrix and its viscoelastic properties that ultimately regulate the bacteria-polymer interactions.
33510716	7	38	theme	formed	1067:1072	arg1	microcolonies					1074:1086	the already formed microcolonies	1055:1086	the already formed microcolonies	1055:1086	Using a population dynamics model, we conclude that hydrodynamic stress causes a faster diffusion of nutrients and a higher incorporation of planktonic bacteria to the already formed microcolonies.
33510716	6	39	theme	weak	785:788	arg1	interaction					811:821	a weak cell-cell attractive interaction	783:821	a weak cell-cell attractive interaction due to the depletion forces originated by the polymer constituents	783:888	At short growth timescales, the matrix mediates a weak cell-cell attractive interaction due to the depletion forces originated by the polymer constituents.
33510716	9	40	theme	adhering	1389:1396	arg1	properties					1398:1407	adhering properties	1389:1407	adhering properties	1389:1407	The mechanical stability also relies on a change in the chemical compositions of the matrix, which becomes enriched in carbohydrates, known to display adhering properties.
33510716	8	41	theme	biofilms	1147:1154	arg1	formation					1109:1117	the formation	1105:1117	the formation of more mechanically stable biofilms due to an increase of the number of crosslinks	1105:1201	This results in the formation of more mechanically stable biofilms due to an increase of the number of crosslinks, as shown by computer simulations.
33510716	9	42	theme	mechanical	1242:1251	arg1	stability					1253:1261	The mechanical stability	1238:1261	The mechanical stability	1238:1261	The mechanical stability also relies on a change in the chemical compositions of the matrix, which becomes enriched in carbohydrates, known to display adhering properties.
33510716	0	43	theme	Hydrodynamic	55:66	arg1	Stress					68:73	Hydrodynamic Stress	55:73	Hydrodynamic Stress	55:73	Self-Adaptation of Pseudomonas fluorescens Biofilms to Hydrodynamic Stress.
33510716	7	44	theme	higher	1008:1013	arg1	incorporation					1015:1027	a higher incorporation	1006:1027	a higher incorporation of planktonic bacteria to the already formed microcolonies	1006:1086	Using a population dynamics model, we conclude that hydrodynamic stress causes a faster diffusion of nutrients and a higher incorporation of planktonic bacteria to the already formed microcolonies.
33510716	8	45	theme	stable	1140:1145	arg1	biofilms					1147:1154	more mechanically stable biofilms	1122:1154	more mechanically stable biofilms due to an increase of the number of crosslinks	1122:1201	This results in the formation of more mechanically stable biofilms due to an increase of the number of crosslinks, as shown by computer simulations.
33510716	6	46	theme	polymer	869:875	arg1	constituents					877:888	the polymer constituents	865:888	the polymer constituents	865:888	At short growth timescales, the matrix mediates a weak cell-cell attractive interaction due to the depletion forces originated by the polymer constituents.
33510716	10	47	theme	hydrodynamic	1488:1499	arg1	stress					1501:1506	hostile hydrodynamic stress	1480:1506	hostile hydrodynamic stress	1480:1506	Overall, we demonstrate that bacteria are capable of self-adapting to hostile hydrodynamic stress by tailoring the biofilm chemical composition, thus affecting both the mesoscale structure of the matrix and its viscoelastic properties that ultimately regulate the bacteria-polymer interactions.
33510716	7	48	theme	planktonic	1032:1041	arg1	bacteria					1043:1050	planktonic bacteria	1032:1050	planktonic bacteria	1032:1050	Using a population dynamics model, we conclude that hydrodynamic stress causes a faster diffusion of nutrients and a higher incorporation of planktonic bacteria to the already formed microcolonies.
33510716	4	49	theme	Pseudomonas	528:538	arg1	biofilms					552:559	Pseudomonas fluorescens biofilms	528:559	Pseudomonas fluorescens biofilms grown with and without hydrodynamic stress	528:602	In this work, we monitor structural features of Pseudomonas fluorescens biofilms grown with and without hydrodynamic stress.
33510716	7	50	theme	bacteria	1043:1050	arg1	incorporation					1015:1027	a higher incorporation	1006:1027	a higher incorporation of planktonic bacteria to the already formed microcolonies	1006:1086	Using a population dynamics model, we conclude that hydrodynamic stress causes a faster diffusion of nutrients and a higher incorporation of planktonic bacteria to the already formed microcolonies.
33510716	7	50	theme	bacteria	1043:1050	arg1	nutrients					992:1000	nutrients	992:1000	nutrients	992:1000	Using a population dynamics model, we conclude that hydrodynamic stress causes a faster diffusion of nutrients and a higher incorporation of planktonic bacteria to the already formed microcolonies.
33510716	1	51	theme	supracellular	134:146	arg1	biofilms					124:131	biofilms	124:131	biofilms	124:131	In some conditions, bacteria self-organize into biofilms, supracellular structures made of a self-produced embedding matrix, mainly composed of polysaccharides, DNA, proteins, and lipids.
33510716	1	51	theme	supracellular	134:146	arg1	structures					148:157	supracellular structures	134:157	supracellular structures made of a self-produced embedding matrix, mainly composed of polysaccharides, DNA, proteins, and lipids	134:261	In some conditions, bacteria self-organize into biofilms, supracellular structures made of a self-produced embedding matrix, mainly composed of polysaccharides, DNA, proteins, and lipids.
33510716	7	52	theme	hydrodynamic	943:954	arg1	stress					956:961	hydrodynamic stress	943:961	hydrodynamic stress	943:961	Using a population dynamics model, we conclude that hydrodynamic stress causes a faster diffusion of nutrients and a higher incorporation of planktonic bacteria to the already formed microcolonies.
33510716	5	53	theme	matrix	716:721	arg1	production					723:732	the matrix production	712:732	the matrix production	712:732	Our measurements show that the hydrodynamic stress concomitantly increases the cell density population and the matrix production.
33510716	7	54	theme	incorporation	1015:1027	arg1	diffusion					979:987	a faster diffusion	970:987	a faster diffusion of nutrients and a higher incorporation of planktonic bacteria to the already formed microcolonies	970:1086	Using a population dynamics model, we conclude that hydrodynamic stress causes a faster diffusion of nutrients and a higher incorporation of planktonic bacteria to the already formed microcolonies.
33510716	10	55	theme	mesoscale	1579:1587	arg1	structure					1589:1597	the mesoscale structure	1575:1597	the mesoscale structure of the matrix	1575:1611	Overall, we demonstrate that bacteria are capable of self-adapting to hostile hydrodynamic stress by tailoring the biofilm chemical composition, thus affecting both the mesoscale structure of the matrix and its viscoelastic properties that ultimately regulate the bacteria-polymer interactions.
33510716	9	56	theme	chemical	1294:1301	arg1	compositions					1303:1314	the chemical compositions	1290:1314	the chemical compositions	1290:1314	The mechanical stability also relies on a change in the chemical compositions of the matrix, which becomes enriched in carbohydrates, known to display adhering properties.
33510716	8	57	theme	due	1156:1158	arg1	biofilms					1147:1154	more mechanically stable biofilms	1122:1154	more mechanically stable biofilms due to an increase of the number of crosslinks	1122:1201	This results in the formation of more mechanically stable biofilms due to an increase of the number of crosslinks, as shown by computer simulations.
31935182	6	0	theme	physiological	893:905	arg1	characteristics					923:937	cultural, physiological and biochemical characteristics	883:937	cultural, physiological and biochemical characteristics that separated it from related Ornithinimicrobium species	883:995	The isolate shared a range of phenotypic patterns reported for members of the genus Ornithinimicrobium, but also had a range of cultural, physiological and biochemical characteristics that separated it from related Ornithinimicrobium species.
31935182	2	1	dep	isolated	163:170	arg1	Lindl					232:236	Lindl	232:236	Lindl	232:236	Strain CPCC 203383T, isolated from the surface-sterilized fruit of Cerasus pseudocerasus (Lindl.)
31935182	2	1	dep	isolated	163:170	arg1	203383T					154:160	Strain CPCC 203383T	142:160	Strain CPCC 203383T	142:160	Strain CPCC 203383T, isolated from the surface-sterilized fruit of Cerasus pseudocerasus (Lindl.)
31935182	11	2	theme	Ornithinimicrobium	1555:1572	arg1	species					1534:1540	a novel species	1526:1540	a novel species	1526:1540	The combined genotypic and phenotypic data indicated that the isolate represents a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cerasi sp.
31935182	5	3	theme	genome	626:631	arg1	analyses					647:654	the whole genome sequence data analyses	616:654	the whole genome sequence data analyses	616:654	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a distinct phyletic branch within the genus Ornithinimicrobium and the whole genome sequence data analyses supported that strain CPCC 203383T was phylogenetically related to the Ornithinimicrobium species.
31935182	6	4	theme	cultural	883:890	arg1	characteristics					923:937	cultural, physiological and biochemical characteristics	883:937	cultural, physiological and biochemical characteristics that separated it from related Ornithinimicrobium species	883:995	The isolate shared a range of phenotypic patterns reported for members of the genus Ornithinimicrobium, but also had a range of cultural, physiological and biochemical characteristics that separated it from related Ornithinimicrobium species.
31935182	4	5	theme	pekingense	396:405	arg1	DSM					407:409	Ornithinimicrobium pekingense DSM 21552	377:415	Ornithinimicrobium pekingense DSM 21552 (97.2 %)	377:424	It had the highest 16S rRNA gene sequence similarities with Ornithinimicrobium pekingense DSM 21552 (97.2 %) and O. kibberense DSM 17687T (97.2%).
31935182	4	5	theme	pekingense	396:405	arg1	%					423:423	97.2 %	418:423	97.2 %	418:423	It had the highest 16S rRNA gene sequence similarities with Ornithinimicrobium pekingense DSM 21552 (97.2 %) and O. kibberense DSM 17687T (97.2%).
31935182	11	6	theme	Ornithinimicrobium	1594:1611	arg1	sp					1620:1621	the name Ornithinimicrobium cerasi sp	1585:1621	the name Ornithinimicrobium cerasi sp	1585:1621	The combined genotypic and phenotypic data indicated that the isolate represents a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cerasi sp.
31935182	5	7	theme	data	642:645	arg1	analyses					647:654	the whole genome sequence data analyses	616:654	the whole genome sequence data analyses	616:654	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a distinct phyletic branch within the genus Ornithinimicrobium and the whole genome sequence data analyses supported that strain CPCC 203383T was phylogenetically related to the Ornithinimicrobium species.
31935182	6	8	theme	patterns	796:803	arg1	range					776:780	a range	774:780	a range of phenotypic patterns reported for members of the genus Ornithinimicrobium	774:856	The isolate shared a range of phenotypic patterns reported for members of the genus Ornithinimicrobium, but also had a range of cultural, physiological and biochemical characteristics that separated it from related Ornithinimicrobium species.
31935182	8	9	theme	unidentified	1152:1163	arg1	ULs					1173:1175	ULs	1173:1175	ULs	1173:1175	The polar lipid profile consisted of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI) and unidentified lipids (ULs).
31935182	8	9	theme	unidentified	1152:1163	arg1	lipids					1165:1170	unidentified lipids	1152:1170	unidentified lipids (ULs)	1152:1176	The polar lipid profile consisted of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI) and unidentified lipids (ULs).
31935182	2	10	theme	Strain	142:147	arg1	Lindl					232:236	Lindl	232:236	Lindl	232:236	Strain CPCC 203383T, isolated from the surface-sterilized fruit of Cerasus pseudocerasus (Lindl.)
31935182	2	10	theme	Strain	142:147	arg1	203383T					154:160	Strain CPCC 203383T	142:160	Strain CPCC 203383T	142:160	Strain CPCC 203383T, isolated from the surface-sterilized fruit of Cerasus pseudocerasus (Lindl.)
31935182	9	11	dep	iso-C15 	1213:1220	arg1	 0					1222:1223	 0	1222:1223	 0	1222:1223	The major fatty acids (>5 %) were iso-C15 : 0, anteiso-C15 : 0, iso-C16:0, 9-methyl C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
31935182	9	11	dep	iso-C15 	1213:1220	arg1	iso-C15 					1213:1220	iso-C15 	1213:1220	iso-C15 	1213:1220	The major fatty acids (>5 %) were iso-C15 : 0, anteiso-C15 : 0, iso-C16:0, 9-methyl C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
31935182	9	11	dep	iso-C15 	1213:1220	arg1	acids					1195:1199	The major fatty acids	1179:1199	The major fatty acids (>5 %)	1179:1206	The major fatty acids (>5 %) were iso-C15 : 0, anteiso-C15 : 0, iso-C16:0, 9-methyl C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
31935182	10	12	theme	wall	1314:1317	arg1	peptidoglycan					1319:1331	The cell wall peptidoglycan	1305:1331	The cell wall peptidoglycan	1305:1331	The cell wall peptidoglycan contains l-ornithine as diagnostic diamino acid and an interpeptide bridge consisting of L-Orn←L-Ala←Gly←D-Asp.
31935182	10	13	contain	contains	1333:1340	arg1	peptidoglycan					1319:1331	The cell wall peptidoglycan	1305:1331	The cell wall peptidoglycan	1305:1331	The cell wall peptidoglycan contains l-ornithine as diagnostic diamino acid and an interpeptide bridge consisting of L-Orn←L-Ala←Gly←D-Asp.
31935182	10	13	contain	contains	1333:1340	arg2	bridge					1401:1406	an interpeptide bridge	1385:1406	an interpeptide bridge consisting of L-Orn←L-Ala←Gly←D-Asp	1385:1442	The cell wall peptidoglycan contains l-ornithine as diagnostic diamino acid and an interpeptide bridge consisting of L-Orn←L-Ala←Gly←D-Asp.
31935182	10	13	contain	contains	1333:1340	arg2	l-ornithine					1342:1352	l-ornithine	1342:1352	l-ornithine	1342:1352	The cell wall peptidoglycan contains l-ornithine as diagnostic diamino acid and an interpeptide bridge consisting of L-Orn←L-Ala←Gly←D-Asp.
31935182	4	14	theme	sequence	350:357	arg1	similarities					359:370	the highest 16S rRNA gene sequence similarities	324:370	the highest 16S rRNA gene sequence similarities	324:370	It had the highest 16S rRNA gene sequence similarities with Ornithinimicrobium pekingense DSM 21552 (97.2 %) and O. kibberense DSM 17687T (97.2%).
31935182	9	15	theme	major	1183:1187	arg1	acids					1195:1199	The major fatty acids	1179:1199	The major fatty acids (>5 %)	1179:1206	The major fatty acids (>5 %) were iso-C15 : 0, anteiso-C15 : 0, iso-C16:0, 9-methyl C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
31935182	9	15	theme	major	1183:1187	arg1	%					1205:1205	>5 %	1202:1205	>5 %	1202:1205	The major fatty acids (>5 %) were iso-C15 : 0, anteiso-C15 : 0, iso-C16:0, 9-methyl C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
31935182	9	15	theme	major	1183:1187	arg1	iso-C15 					1213:1220	iso-C15 	1213:1220	iso-C15 	1213:1220	The major fatty acids (>5 %) were iso-C15 : 0, anteiso-C15 : 0, iso-C16:0, 9-methyl C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
31935182	9	15	theme	major	1183:1187	arg1	 0					1222:1223	 0	1222:1223	 0	1222:1223	The major fatty acids (>5 %) were iso-C15 : 0, anteiso-C15 : 0, iso-C16:0, 9-methyl C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
31935182	6	16	theme	related	962:968	arg1	species					989:995	related Ornithinimicrobium species	962:995	related Ornithinimicrobium species	962:995	The isolate shared a range of phenotypic patterns reported for members of the genus Ornithinimicrobium, but also had a range of cultural, physiological and biochemical characteristics that separated it from related Ornithinimicrobium species.
31935182	12	17	theme	=NBRC	1660:1664	arg1	203383T					1652:1658	CPCC 203383T	1647:1658	CPCC 203383T(=NBRC 113522T=KCTC 49200T) as the type strain	1647:1704	nov. is proposed, with CPCC 203383T(=NBRC 113522T=KCTC 49200T) as the type strain.
31935182	12	17	theme	=NBRC	1660:1664	arg1	49200T					1679:1684	=NBRC 113522T=KCTC 49200T	1660:1684	=NBRC 113522T=KCTC 49200T	1660:1684	nov. is proposed, with CPCC 203383T(=NBRC 113522T=KCTC 49200T) as the type strain.
31935182	6	18	contain	had	868:870	arg2	range					874:878	a range	872:878	a range of cultural, physiological and biochemical characteristics that separated it from related Ornithinimicrobium species	872:995	The isolate shared a range of phenotypic patterns reported for members of the genus Ornithinimicrobium, but also had a range of cultural, physiological and biochemical characteristics that separated it from related Ornithinimicrobium species.
31935182	6	18	contain	had	868:870	arg1	isolate					759:765	The isolate	755:765	The isolate	755:765	The isolate shared a range of phenotypic patterns reported for members of the genus Ornithinimicrobium, but also had a range of cultural, physiological and biochemical characteristics that separated it from related Ornithinimicrobium species.
31935182	4	19	theme	O.	430:431	arg1	%					460:460	97.2%	456:460	97.2%	456:460	It had the highest 16S rRNA gene sequence similarities with Ornithinimicrobium pekingense DSM 21552 (97.2 %) and O. kibberense DSM 17687T (97.2%).
31935182	4	19	theme	O.	430:431	arg1	17687T					448:453	O. kibberense DSM 17687T	430:453	O. kibberense DSM 17687T (97.2%)	430:461	It had the highest 16S rRNA gene sequence similarities with Ornithinimicrobium pekingense DSM 21552 (97.2 %) and O. kibberense DSM 17687T (97.2%).
31935182	3	20	dep	G.	240:241	arg1	Don					243:245	Don	243:245	Don	243:245	G. Don, was taxonomically characterized based on a polyphasic investigation.
31935182	3	21	theme	polyphasic	291:300	arg1	investigation					302:314	a polyphasic investigation	289:314	a polyphasic investigation	289:314	G. Don, was taxonomically characterized based on a polyphasic investigation.
31935182	4	22	theme	DSM	444:446	arg1	%					460:460	97.2%	456:460	97.2%	456:460	It had the highest 16S rRNA gene sequence similarities with Ornithinimicrobium pekingense DSM 21552 (97.2 %) and O. kibberense DSM 17687T (97.2%).
31935182	4	22	theme	DSM	444:446	arg1	17687T					448:453	O. kibberense DSM 17687T	430:453	O. kibberense DSM 17687T (97.2%)	430:461	It had the highest 16S rRNA gene sequence similarities with Ornithinimicrobium pekingense DSM 21552 (97.2 %) and O. kibberense DSM 17687T (97.2%).
31935182	2	23	theme	pseudocerasus	217:229	arg1	fruit					200:204	the surface-sterilized fruit	177:204	the surface-sterilized fruit of Cerasus pseudocerasus	177:229	Strain CPCC 203383T, isolated from the surface-sterilized fruit of Cerasus pseudocerasus (Lindl.)
31935182	14	24	theme	new	1765:1767	arg1	data					1769:1772	new data	1765:1772	new data	1765:1772	The availability of new data allows for an emended description of the genus Ornithinimicrobium.
31935182	6	25	theme	biochemical	911:921	arg1	characteristics					923:937	cultural, physiological and biochemical characteristics	883:937	cultural, physiological and biochemical characteristics that separated it from related Ornithinimicrobium species	883:995	The isolate shared a range of phenotypic patterns reported for members of the genus Ornithinimicrobium, but also had a range of cultural, physiological and biochemical characteristics that separated it from related Ornithinimicrobium species.
31935182	5	26	theme	strain	671:676	arg1	203383T					683:689	strain CPCC 203383T	671:689	strain CPCC 203383T	671:689	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a distinct phyletic branch within the genus Ornithinimicrobium and the whole genome sequence data analyses supported that strain CPCC 203383T was phylogenetically related to the Ornithinimicrobium species.
31935182	1	27	theme	Ornithinimicrobium	122:139	arg1	fruit					54:58	the fruit	50:58	the fruit of Cerasus pseudocerasus	50:83	nov., isolated from the fruit of Cerasus pseudocerasus and emended description of the genus Ornithinimicrobium.
31935182	1	27	theme	Ornithinimicrobium	122:139	arg1	description					97:107	emended description	89:107	emended description of the genus Ornithinimicrobium	89:139	nov., isolated from the fruit of Cerasus pseudocerasus and emended description of the genus Ornithinimicrobium.
31935182	12	28	theme	CPCC	1647:1650	arg1	203383T					1652:1658	CPCC 203383T	1647:1658	CPCC 203383T(=NBRC 113522T=KCTC 49200T) as the type strain	1647:1704	nov. is proposed, with CPCC 203383T(=NBRC 113522T=KCTC 49200T) as the type strain.
31935182	12	28	theme	CPCC	1647:1650	arg1	49200T					1679:1684	=NBRC 113522T=KCTC 49200T	1660:1684	=NBRC 113522T=KCTC 49200T	1660:1684	nov. is proposed, with CPCC 203383T(=NBRC 113522T=KCTC 49200T) as the type strain.
31935182	4	29	contain	had	320:322	arg1	It					317:318	It	317:318	It	317:318	It had the highest 16S rRNA gene sequence similarities with Ornithinimicrobium pekingense DSM 21552 (97.2 %) and O. kibberense DSM 17687T (97.2%).
31935182	4	29	contain	had	320:322	arg2	similarities					359:370	the highest 16S rRNA gene sequence similarities	324:370	the highest 16S rRNA gene sequence similarities	324:370	It had the highest 16S rRNA gene sequence similarities with Ornithinimicrobium pekingense DSM 21552 (97.2 %) and O. kibberense DSM 17687T (97.2%).
31935182	5	30	theme	rRNA	499:502	arg1	sequences					509:517	16S rRNA gene sequences	495:517	16S rRNA gene sequences	495:517	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a distinct phyletic branch within the genus Ornithinimicrobium and the whole genome sequence data analyses supported that strain CPCC 203383T was phylogenetically related to the Ornithinimicrobium species.
31935182	5	31	theme	phyletic	560:567	arg1	branch					569:574	a distinct phyletic branch	549:574	a distinct phyletic branch within the genus Ornithinimicrobium	549:610	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a distinct phyletic branch within the genus Ornithinimicrobium and the whole genome sequence data analyses supported that strain CPCC 203383T was phylogenetically related to the Ornithinimicrobium species.
31935182	11	32	theme	combined	1449:1456	arg1	data					1483:1486	The combined genotypic and phenotypic data	1445:1486	The combined genotypic and phenotypic data	1445:1486	The combined genotypic and phenotypic data indicated that the isolate represents a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cerasi sp.
31935182	5	33	theme	Ornithinimicrobium	727:744	arg1	species					746:752	the Ornithinimicrobium species	723:752	the Ornithinimicrobium species	723:752	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a distinct phyletic branch within the genus Ornithinimicrobium and the whole genome sequence data analyses supported that strain CPCC 203383T was phylogenetically related to the Ornithinimicrobium species.
31935182	8	34	theme	lipid	1038:1042	arg1	profile					1044:1050	The polar lipid profile	1028:1050	The polar lipid profile	1028:1050	The polar lipid profile consisted of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI) and unidentified lipids (ULs).
31935182	10	35	theme	diamino	1368:1374	arg1	acid					1376:1379	diagnostic diamino acid	1357:1379	diagnostic diamino acid	1357:1379	The cell wall peptidoglycan contains l-ornithine as diagnostic diamino acid and an interpeptide bridge consisting of L-Orn←L-Ala←Gly←D-Asp.
31935182	11	36	theme	cerasi	1613:1618	arg1	sp					1620:1621	the name Ornithinimicrobium cerasi sp	1585:1621	the name Ornithinimicrobium cerasi sp	1585:1621	The combined genotypic and phenotypic data indicated that the isolate represents a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cerasi sp.
31935182	0	37	theme	Ornithinimicrobium	0:17	arg1	sp					26:27	Ornithinimicrobium cerasi sp	0:27	Ornithinimicrobium cerasi sp.	0:28	Ornithinimicrobium cerasi sp.
31935182	9	38	theme	9-methyl	1254:1261	arg1	C16 					1263:1266	9-methyl C16 	1254:1266	9-methyl C16 	1254:1266	The major fatty acids (>5 %) were iso-C15 : 0, anteiso-C15 : 0, iso-C16:0, 9-methyl C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
31935182	9	38	theme	9-methyl	1254:1261	arg1	 0					1239:1240	 0	1239:1240	 0	1239:1240	The major fatty acids (>5 %) were iso-C15 : 0, anteiso-C15 : 0, iso-C16:0, 9-methyl C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
31935182	6	39	theme	phenotypic	785:794	arg1	patterns					796:803	phenotypic patterns	785:803	phenotypic patterns reported for members of the genus Ornithinimicrobium	785:856	The isolate shared a range of phenotypic patterns reported for members of the genus Ornithinimicrobium, but also had a range of cultural, physiological and biochemical characteristics that separated it from related Ornithinimicrobium species.
31935182	10	40	theme	interpeptide	1388:1399	arg1	bridge					1401:1406	an interpeptide bridge	1385:1406	an interpeptide bridge consisting of L-Orn←L-Ala←Gly←D-Asp	1385:1442	The cell wall peptidoglycan contains l-ornithine as diagnostic diamino acid and an interpeptide bridge consisting of L-Orn←L-Ala←Gly←D-Asp.
31935182	10	40	theme	interpeptide	1388:1399	arg1	l-ornithine					1342:1352	l-ornithine	1342:1352	l-ornithine	1342:1352	The cell wall peptidoglycan contains l-ornithine as diagnostic diamino acid and an interpeptide bridge consisting of L-Orn←L-Ala←Gly←D-Asp.
31935182	4	41	theme	16S	336:338	arg1	rRNA					340:343	the highest 16S rRNA	324:343	the highest 16S rRNA gene sequence similarities	324:370	It had the highest 16S rRNA gene sequence similarities with Ornithinimicrobium pekingense DSM 21552 (97.2 %) and O. kibberense DSM 17687T (97.2%).
31935182	2	42	attach	isolated	163:170	arg2	203383T					154:160	Strain CPCC 203383T	142:160	Strain CPCC 203383T	142:160	Strain CPCC 203383T, isolated from the surface-sterilized fruit of Cerasus pseudocerasus (Lindl.)
31935182	2	42	attach	isolated	163:170	arg1	fruit					200:204	the surface-sterilized fruit	177:204	the surface-sterilized fruit of Cerasus pseudocerasus	177:229	Strain CPCC 203383T, isolated from the surface-sterilized fruit of Cerasus pseudocerasus (Lindl.)
31935182	2	42	attach	isolated	163:170	arg2	Lindl					232:236	Lindl	232:236	Lindl	232:236	Strain CPCC 203383T, isolated from the surface-sterilized fruit of Cerasus pseudocerasus (Lindl.)
31935182	11	43	theme	novel	1528:1532	arg1	species					1534:1540	a novel species	1526:1540	a novel species	1526:1540	The combined genotypic and phenotypic data indicated that the isolate represents a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cerasi sp.
31935182	6	44	theme	genus	833:837	arg1	Ornithinimicrobium					839:856	the genus Ornithinimicrobium	829:856	the genus Ornithinimicrobium	829:856	The isolate shared a range of phenotypic patterns reported for members of the genus Ornithinimicrobium, but also had a range of cultural, physiological and biochemical characteristics that separated it from related Ornithinimicrobium species.
31935182	4	45	theme	gene	345:348	arg1	similarities					359:370	the highest 16S rRNA gene sequence similarities	324:370	the highest 16S rRNA gene sequence similarities	324:370	It had the highest 16S rRNA gene sequence similarities with Ornithinimicrobium pekingense DSM 21552 (97.2 %) and O. kibberense DSM 17687T (97.2%).
31935182	13	46	theme	DNA	1711:1713	arg1	composition					1719:1729	The DNA G+C composition	1707:1729	The DNA G+C composition	1707:1729	The DNA G+C composition is 72.3 mol%.
31935182	13	46	theme	DNA	1711:1713	arg1	%					1742:1742	72.3 mol%	1734:1742	72.3 mol%	1734:1742	The DNA G+C composition is 72.3 mol%.
31935182	2	47	theme	surface-sterilized	181:198	arg1	fruit					200:204	the surface-sterilized fruit	177:204	the surface-sterilized fruit of Cerasus pseudocerasus	177:229	Strain CPCC 203383T, isolated from the surface-sterilized fruit of Cerasus pseudocerasus (Lindl.)
31935182	11	48	theme	genus	1549:1553	arg1	Ornithinimicrobium					1555:1572	the genus Ornithinimicrobium	1545:1572	the genus Ornithinimicrobium	1545:1572	The combined genotypic and phenotypic data indicated that the isolate represents a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cerasi sp.
31935182	14	49	theme	Ornithinimicrobium	1821:1838	arg1	description					1796:1806	an emended description	1785:1806	an emended description of the genus Ornithinimicrobium	1785:1838	The availability of new data allows for an emended description of the genus Ornithinimicrobium.
31935182	5	50	theme	whole	620:624	arg1	analyses					647:654	the whole genome sequence data analyses	616:654	the whole genome sequence data analyses	616:654	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a distinct phyletic branch within the genus Ornithinimicrobium and the whole genome sequence data analyses supported that strain CPCC 203383T was phylogenetically related to the Ornithinimicrobium species.
31935182	5	51	theme	sequence	633:640	arg1	analyses					647:654	the whole genome sequence data analyses	616:654	the whole genome sequence data analyses	616:654	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a distinct phyletic branch within the genus Ornithinimicrobium and the whole genome sequence data analyses supported that strain CPCC 203383T was phylogenetically related to the Ornithinimicrobium species.
31935182	11	52	theme	phenotypic	1472:1481	arg1	data					1483:1486	The combined genotypic and phenotypic data	1445:1486	The combined genotypic and phenotypic data	1445:1486	The combined genotypic and phenotypic data indicated that the isolate represents a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cerasi sp.
31935182	2	53	theme	CPCC	149:152	arg1	Lindl					232:236	Lindl	232:236	Lindl	232:236	Strain CPCC 203383T, isolated from the surface-sterilized fruit of Cerasus pseudocerasus (Lindl.)
31935182	2	53	theme	CPCC	149:152	arg1	203383T					154:160	Strain CPCC 203383T	142:160	Strain CPCC 203383T	142:160	Strain CPCC 203383T, isolated from the surface-sterilized fruit of Cerasus pseudocerasus (Lindl.)
31935182	5	54	theme	genus	587:591	arg1	Ornithinimicrobium					593:610	the genus Ornithinimicrobium	583:610	the genus Ornithinimicrobium	583:610	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a distinct phyletic branch within the genus Ornithinimicrobium and the whole genome sequence data analyses supported that strain CPCC 203383T was phylogenetically related to the Ornithinimicrobium species.
31935182	11	55	theme	genotypic	1458:1466	arg1	data					1483:1486	The combined genotypic and phenotypic data	1445:1486	The combined genotypic and phenotypic data	1445:1486	The combined genotypic and phenotypic data indicated that the isolate represents a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cerasi sp.
31935182	5	56	theme	Phylogenetic	464:475	arg1	analysis					477:484	Phylogenetic analysis	464:484	Phylogenetic analysis based on 16S rRNA gene sequences	464:517	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a distinct phyletic branch within the genus Ornithinimicrobium and the whole genome sequence data analyses supported that strain CPCC 203383T was phylogenetically related to the Ornithinimicrobium species.
31935182	14	57	theme	genus	1815:1819	arg1	Ornithinimicrobium					1821:1838	the genus Ornithinimicrobium	1811:1838	the genus Ornithinimicrobium	1811:1838	The availability of new data allows for an emended description of the genus Ornithinimicrobium.
31935182	11	58	theme	name	1589:1592	arg1	sp					1620:1621	the name Ornithinimicrobium cerasi sp	1585:1621	the name Ornithinimicrobium cerasi sp	1585:1621	The combined genotypic and phenotypic data indicated that the isolate represents a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium cerasi sp.
31935182	10	59	theme	cell	1309:1312	arg1	peptidoglycan					1319:1331	The cell wall peptidoglycan	1305:1331	The cell wall peptidoglycan	1305:1331	The cell wall peptidoglycan contains l-ornithine as diagnostic diamino acid and an interpeptide bridge consisting of L-Orn←L-Ala←Gly←D-Asp.
31935182	6	60	theme	Ornithinimicrobium	970:987	arg1	species					989:995	related Ornithinimicrobium species	962:995	related Ornithinimicrobium species	962:995	The isolate shared a range of phenotypic patterns reported for members of the genus Ornithinimicrobium, but also had a range of cultural, physiological and biochemical characteristics that separated it from related Ornithinimicrobium species.
31935182	4	61	theme	Ornithinimicrobium	377:394	arg1	DSM					407:409	Ornithinimicrobium pekingense DSM 21552	377:415	Ornithinimicrobium pekingense DSM 21552 (97.2 %)	377:424	It had the highest 16S rRNA gene sequence similarities with Ornithinimicrobium pekingense DSM 21552 (97.2 %) and O. kibberense DSM 17687T (97.2%).
31935182	4	61	theme	Ornithinimicrobium	377:394	arg1	%					423:423	97.2 %	418:423	97.2 %	418:423	It had the highest 16S rRNA gene sequence similarities with Ornithinimicrobium pekingense DSM 21552 (97.2 %) and O. kibberense DSM 17687T (97.2%).
31935182	12	62	theme	113522T=KCTC	1666:1677	arg1	203383T					1652:1658	CPCC 203383T	1647:1658	CPCC 203383T(=NBRC 113522T=KCTC 49200T) as the type strain	1647:1704	nov. is proposed, with CPCC 203383T(=NBRC 113522T=KCTC 49200T) as the type strain.
31935182	12	62	theme	113522T=KCTC	1666:1677	arg1	49200T					1679:1684	=NBRC 113522T=KCTC 49200T	1660:1684	=NBRC 113522T=KCTC 49200T	1660:1684	nov. is proposed, with CPCC 203383T(=NBRC 113522T=KCTC 49200T) as the type strain.
31935182	5	63	theme	CPCC	678:681	arg1	203383T					683:689	strain CPCC 203383T	671:689	strain CPCC 203383T	671:689	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a distinct phyletic branch within the genus Ornithinimicrobium and the whole genome sequence data analyses supported that strain CPCC 203383T was phylogenetically related to the Ornithinimicrobium species.
31935182	9	64	theme	fatty	1189:1193	arg1	acids					1195:1199	The major fatty acids	1179:1199	The major fatty acids (>5 %)	1179:1206	The major fatty acids (>5 %) were iso-C15 : 0, anteiso-C15 : 0, iso-C16:0, 9-methyl C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
31935182	9	64	theme	fatty	1189:1193	arg1	%					1205:1205	>5 %	1202:1205	>5 %	1202:1205	The major fatty acids (>5 %) were iso-C15 : 0, anteiso-C15 : 0, iso-C16:0, 9-methyl C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
31935182	9	64	theme	fatty	1189:1193	arg1	iso-C15 					1213:1220	iso-C15 	1213:1220	iso-C15 	1213:1220	The major fatty acids (>5 %) were iso-C15 : 0, anteiso-C15 : 0, iso-C16:0, 9-methyl C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
31935182	9	64	theme	fatty	1189:1193	arg1	 0					1222:1223	 0	1222:1223	 0	1222:1223	The major fatty acids (>5 %) were iso-C15 : 0, anteiso-C15 : 0, iso-C16:0, 9-methyl C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
31935182	14	65	theme	emended	1788:1794	arg1	description					1796:1806	an emended description	1785:1806	an emended description of the genus Ornithinimicrobium	1785:1838	The availability of new data allows for an emended description of the genus Ornithinimicrobium.
31935182	4	66	theme	kibberense	433:442	arg1	%					460:460	97.2%	456:460	97.2%	456:460	It had the highest 16S rRNA gene sequence similarities with Ornithinimicrobium pekingense DSM 21552 (97.2 %) and O. kibberense DSM 17687T (97.2%).
31935182	4	66	theme	kibberense	433:442	arg1	17687T					448:453	O. kibberense DSM 17687T	430:453	O. kibberense DSM 17687T (97.2%)	430:461	It had the highest 16S rRNA gene sequence similarities with Ornithinimicrobium pekingense DSM 21552 (97.2 %) and O. kibberense DSM 17687T (97.2%).
31935182	14	67	theme	data	1769:1772	arg1	availability					1749:1760	The availability	1745:1760	The availability of new data	1745:1772	The availability of new data allows for an emended description of the genus Ornithinimicrobium.
31935182	6	68	theme	characteristics	923:937	arg1	range					874:878	a range	872:878	a range of cultural, physiological and biochemical characteristics that separated it from related Ornithinimicrobium species	872:995	The isolate shared a range of phenotypic patterns reported for members of the genus Ornithinimicrobium, but also had a range of cultural, physiological and biochemical characteristics that separated it from related Ornithinimicrobium species.
31935182	1	69	theme	genus	116:120	arg1	Ornithinimicrobium					122:139	the genus Ornithinimicrobium	112:139	the genus Ornithinimicrobium	112:139	nov., isolated from the fruit of Cerasus pseudocerasus and emended description of the genus Ornithinimicrobium.
31935182	5	70	theme	16S	495:497	arg1	sequences					509:517	16S rRNA gene sequences	495:517	16S rRNA gene sequences	495:517	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a distinct phyletic branch within the genus Ornithinimicrobium and the whole genome sequence data analyses supported that strain CPCC 203383T was phylogenetically related to the Ornithinimicrobium species.
31935182	5	71	theme	gene	504:507	arg1	sequences					509:517	16S rRNA gene sequences	495:517	16S rRNA gene sequences	495:517	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a distinct phyletic branch within the genus Ornithinimicrobium and the whole genome sequence data analyses supported that strain CPCC 203383T was phylogenetically related to the Ornithinimicrobium species.
31935182	8	72	theme	polar	1032:1036	arg1	profile					1044:1050	The polar lipid profile	1028:1050	The polar lipid profile	1028:1050	The polar lipid profile consisted of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylinositol (PI) and unidentified lipids (ULs).
31935182	0	73	theme	cerasi	19:24	arg1	sp					26:27	Ornithinimicrobium cerasi sp	0:27	Ornithinimicrobium cerasi sp.	0:28	Ornithinimicrobium cerasi sp.
31935182	1	74	theme	pseudocerasus	71:83	arg1	fruit					54:58	the fruit	50:58	the fruit of Cerasus pseudocerasus	50:83	nov., isolated from the fruit of Cerasus pseudocerasus and emended description of the genus Ornithinimicrobium.
31935182	1	74	theme	pseudocerasus	71:83	arg1	description					97:107	emended description	89:107	emended description of the genus Ornithinimicrobium	89:139	nov., isolated from the fruit of Cerasus pseudocerasus and emended description of the genus Ornithinimicrobium.
31935182	10	75	theme	diagnostic	1357:1366	arg1	acid					1376:1379	diagnostic diamino acid	1357:1379	diagnostic diamino acid	1357:1379	The cell wall peptidoglycan contains l-ornithine as diagnostic diamino acid and an interpeptide bridge consisting of L-Orn←L-Ala←Gly←D-Asp.
31935182	13	76	theme	72.3 mol	1734:1741	arg1	composition					1719:1729	The DNA G+C composition	1707:1729	The DNA G+C composition	1707:1729	The DNA G+C composition is 72.3 mol%.
31935182	13	76	theme	72.3 mol	1734:1741	arg1	%					1742:1742	72.3 mol%	1734:1742	72.3 mol%	1734:1742	The DNA G+C composition is 72.3 mol%.
31935182	5	77	theme	distinct	551:558	arg1	branch					569:574	a distinct phyletic branch	549:574	a distinct phyletic branch within the genus Ornithinimicrobium	549:610	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a distinct phyletic branch within the genus Ornithinimicrobium and the whole genome sequence data analyses supported that strain CPCC 203383T was phylogenetically related to the Ornithinimicrobium species.
31935182	1	78	theme	emended	89:95	arg1	description					97:107	emended description	89:107	emended description of the genus Ornithinimicrobium	89:139	nov., isolated from the fruit of Cerasus pseudocerasus and emended description of the genus Ornithinimicrobium.
31935182	9	79	dep	 0	1222:1223	arg1	 0					1301:1302	 0	1301:1302	 0	1301:1302	The major fatty acids (>5 %) were iso-C15 : 0, anteiso-C15 : 0, iso-C16:0, 9-methyl C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
31935182	9	79	dep	 0	1222:1223	arg1	iso-C16:0					1243:1251	iso-C16:0	1243:1251	iso-C16:0	1243:1251	The major fatty acids (>5 %) were iso-C15 : 0, anteiso-C15 : 0, iso-C16:0, 9-methyl C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
31935182	9	79	dep	 0	1222:1223	arg1	 0					1281:1282	 0	1281:1282	 0	1281:1282	The major fatty acids (>5 %) were iso-C15 : 0, anteiso-C15 : 0, iso-C16:0, 9-methyl C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
31935182	9	79	dep	 0	1222:1223	arg1	C16 					1263:1266	9-methyl C16 	1254:1266	9-methyl C16 	1254:1266	The major fatty acids (>5 %) were iso-C15 : 0, anteiso-C15 : 0, iso-C16:0, 9-methyl C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
31935182	9	79	dep	 0	1222:1223	arg1	 0					1268:1269	 0	1268:1269	 0	1268:1269	The major fatty acids (>5 %) were iso-C15 : 0, anteiso-C15 : 0, iso-C16:0, 9-methyl C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
31935182	9	79	dep	 0	1222:1223	arg1	anteiso-C17 					1288:1299	anteiso-C17 	1288:1299	anteiso-C17 	1288:1299	The major fatty acids (>5 %) were iso-C15 : 0, anteiso-C15 : 0, iso-C16:0, 9-methyl C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
31935182	9	79	dep	 0	1222:1223	arg1	 0					1239:1240	 0	1239:1240	 0	1239:1240	The major fatty acids (>5 %) were iso-C15 : 0, anteiso-C15 : 0, iso-C16:0, 9-methyl C16 : 0, iso-C17 : 0 and anteiso-C17 : 0.
31935182	6	80	theme	Ornithinimicrobium	839:856	arg1	members					818:824	members	818:824	members of the genus Ornithinimicrobium	818:856	The isolate shared a range of phenotypic patterns reported for members of the genus Ornithinimicrobium, but also had a range of cultural, physiological and biochemical characteristics that separated it from related Ornithinimicrobium species.
31935182	4	81	theme	highest	328:334	arg1	rRNA					340:343	the highest 16S rRNA	324:343	the highest 16S rRNA gene sequence similarities	324:370	It had the highest 16S rRNA gene sequence similarities with Ornithinimicrobium pekingense DSM 21552 (97.2 %) and O. kibberense DSM 17687T (97.2%).
31935182	2	82	theme	Cerasus	209:215	arg1	pseudocerasus					217:229	Cerasus pseudocerasus	209:229	Cerasus pseudocerasus	209:229	Strain CPCC 203383T, isolated from the surface-sterilized fruit of Cerasus pseudocerasus (Lindl.)
31935182	4	83	theme	rRNA	340:343	arg1	similarities					359:370	the highest 16S rRNA gene sequence similarities	324:370	the highest 16S rRNA gene sequence similarities	324:370	It had the highest 16S rRNA gene sequence similarities with Ornithinimicrobium pekingense DSM 21552 (97.2 %) and O. kibberense DSM 17687T (97.2%).
31935182	12	84	theme	type	1694:1697	arg1	strain					1699:1704	the type strain	1690:1704	the type strain	1690:1704	nov. is proposed, with CPCC 203383T(=NBRC 113522T=KCTC 49200T) as the type strain.
31935182	13	85	theme	G+C	1715:1717	arg1	composition					1719:1729	The DNA G+C composition	1707:1729	The DNA G+C composition	1707:1729	The DNA G+C composition is 72.3 mol%.
31935182	13	85	theme	G+C	1715:1717	arg1	%					1742:1742	72.3 mol%	1734:1742	72.3 mol%	1734:1742	The DNA G+C composition is 72.3 mol%.
34314799	3	0	theme	gel	366:368	arg1	strength					370:377	The highest gel strength	354:377	The highest gel strength	354:377	The highest gel strength was observed at a Fmoc-FF mass fraction (ϕFF) of 0.85, whereby the highest combined strength of the two interactions was achieved.
34314799	5	1	theme	cationic	643:650	arg1	drug					658:661	a cationic model drug	641:661	a cationic model drug	641:661	The incorporation of doxorubicin (DOX) as a cationic model drug significantly increased the stability of the complex hydrogels.
34314799	6	2	theme	DOX-loaded	727:736	arg1	hydrogels					738:746	DOX-loaded hydrogels	727:746	DOX-loaded hydrogels	727:746	DOX-loaded hydrogels exhibited slow DOX release, probably due to the drug's strong binding to Fmoc-FF via electrostatic attraction and the high gel stability.
34314799	1	3	theme	diphenylalanine	205:219	arg1	interaction					253:263	its electrostatic interaction	235:263	its electrostatic interaction with glycol chitosan (GCS)	235:290	A novel chitosan-dipeptide hydrogel was fabricated through a combination of self-assembly of 9-fluorenylmethoxycarbonyl-modified diphenylalanine (Fmoc-FF) and its electrostatic interaction with glycol chitosan (GCS).
34314799	1	3	theme	diphenylalanine	205:219	arg1	self-assembly					152:164	self-assembly	152:164	self-assembly of 9-fluorenylmethoxycarbonyl-modified diphenylalanine (Fmoc-FF)	152:229	A novel chitosan-dipeptide hydrogel was fabricated through a combination of self-assembly of 9-fluorenylmethoxycarbonyl-modified diphenylalanine (Fmoc-FF) and its electrostatic interaction with glycol chitosan (GCS).
34314799	8	4	theme	medium	1099:1104	arg1	pH					1089:1090	the pH	1085:1090	the pH of the medium	1085:1104	Notably, DOX release was significantly accelerated as the pH of the medium decreased from 7.46 to 5.5 and 4.0, possibly due to hydrogel components' protonation.
34314799	9	5	theme	developed	1310:1318	arg1	hydrogel					1320:1327	the newly developed hydrogel	1300:1327	the newly developed hydrogel to be a promising candidate vehicle for the localized and controlled drug delivery in cancer therapy	1300:1428	The DOX-loaded hydrogel exhibited notable cytotoxicity against A549 human lung cancer cells, which suggests the newly developed hydrogel to be a promising candidate vehicle for the localized and controlled drug delivery in cancer therapy.
34314799	5	6	theme	model	652:656	arg1	drug					658:661	a cationic model drug	641:661	a cationic model drug	641:661	The incorporation of doxorubicin (DOX) as a cationic model drug significantly increased the stability of the complex hydrogels.
34314799	7	7	theme	injectable	984:993	arg1	systems					1022:1028	injectable self-healing drug delivery systems	984:1028	injectable self-healing drug delivery systems	984:1028	These hydrogels also exhibited excellent thixotropic features that facilitated the development of injectable self-healing drug delivery systems.
34314799	9	8	dep	exhibited	1216:1224	arg1	suggests					1291:1298	suggests	1291:1298	suggests the newly developed hydrogel to be a promising candidate vehicle for the localized and controlled drug delivery in cancer therapy	1291:1428	The DOX-loaded hydrogel exhibited notable cytotoxicity against A549 human lung cancer cells, which suggests the newly developed hydrogel to be a promising candidate vehicle for the localized and controlled drug delivery in cancer therapy.
34314799	7	9	theme	systems	1022:1028	arg1	development					969:979	the development	965:979	the development of injectable self-healing drug delivery systems	965:1028	These hydrogels also exhibited excellent thixotropic features that facilitated the development of injectable self-healing drug delivery systems.
34314799	3	10	theme	Fmoc-FF	397:403	arg1	ϕFF					420:422	ϕFF	420:422	ϕFF	420:422	The highest gel strength was observed at a Fmoc-FF mass fraction (ϕFF) of 0.85, whereby the highest combined strength of the two interactions was achieved.
34314799	3	10	theme	Fmoc-FF	397:403	arg1	fraction					410:417	a Fmoc-FF mass fraction	395:417	a Fmoc-FF mass fraction (ϕFF)	395:423	The highest gel strength was observed at a Fmoc-FF mass fraction (ϕFF) of 0.85, whereby the highest combined strength of the two interactions was achieved.
34314799	7	11	theme	self-healing	995:1006	arg1	systems					1022:1028	injectable self-healing drug delivery systems	984:1028	injectable self-healing drug delivery systems	984:1028	These hydrogels also exhibited excellent thixotropic features that facilitated the development of injectable self-healing drug delivery systems.
34314799	6	12	theme	strong	803:808	arg1	binding					810:816	the drug's strong binding	792:816	the drug's strong binding to Fmoc-FF via electrostatic attraction	792:856	DOX-loaded hydrogels exhibited slow DOX release, probably due to the drug's strong binding to Fmoc-FF via electrostatic attraction and the high gel stability.
34314799	7	13	theme	delivery	1013:1020	arg1	systems					1022:1028	injectable self-healing drug delivery systems	984:1028	injectable self-healing drug delivery systems	984:1028	These hydrogels also exhibited excellent thixotropic features that facilitated the development of injectable self-healing drug delivery systems.
34314799	3	14	theme	mass	405:408	arg1	ϕFF					420:422	ϕFF	420:422	ϕFF	420:422	The highest gel strength was observed at a Fmoc-FF mass fraction (ϕFF) of 0.85, whereby the highest combined strength of the two interactions was achieved.
34314799	3	14	theme	mass	405:408	arg1	fraction					410:417	a Fmoc-FF mass fraction	395:417	a Fmoc-FF mass fraction (ϕFF)	395:423	The highest gel strength was observed at a Fmoc-FF mass fraction (ϕFF) of 0.85, whereby the highest combined strength of the two interactions was achieved.
34314799	3	15	located	observed	383:390	arg1	ϕFF					420:422	ϕFF	420:422	ϕFF	420:422	The highest gel strength was observed at a Fmoc-FF mass fraction (ϕFF) of 0.85, whereby the highest combined strength of the two interactions was achieved.
34314799	3	15	located	observed	383:390	arg1	fraction					410:417	a Fmoc-FF mass fraction	395:417	a Fmoc-FF mass fraction (ϕFF)	395:423	The highest gel strength was observed at a Fmoc-FF mass fraction (ϕFF) of 0.85, whereby the highest combined strength of the two interactions was achieved.
34314799	3	15	located	observed	383:390	arg2	strength					370:377	The highest gel strength	354:377	The highest gel strength	354:377	The highest gel strength was observed at a Fmoc-FF mass fraction (ϕFF) of 0.85, whereby the highest combined strength of the two interactions was achieved.
34314799	5	16	theme	doxorubicin	620:630	arg1	incorporation					603:615	The incorporation	599:615	The incorporation of doxorubicin (DOX) as a cationic model drug	599:661	The incorporation of doxorubicin (DOX) as a cationic model drug significantly increased the stability of the complex hydrogels.
34314799	3	17	theme	interactions	483:494	arg1	strength					463:470	the highest combined strength	442:470	the highest combined strength of the two interactions	442:494	The highest gel strength was observed at a Fmoc-FF mass fraction (ϕFF) of 0.85, whereby the highest combined strength of the two interactions was achieved.
34314799	1	18	theme	novel	78:82	arg1	hydrogel					103:110	A novel chitosan-dipeptide hydrogel	76:110	A novel chitosan-dipeptide hydrogel	76:110	A novel chitosan-dipeptide hydrogel was fabricated through a combination of self-assembly of 9-fluorenylmethoxycarbonyl-modified diphenylalanine (Fmoc-FF) and its electrostatic interaction with glycol chitosan (GCS).
34314799	3	19	theme	combined	454:461	arg1	strength					463:470	the highest combined strength	442:470	the highest combined strength of the two interactions	442:494	The highest gel strength was observed at a Fmoc-FF mass fraction (ϕFF) of 0.85, whereby the highest combined strength of the two interactions was achieved.
34314799	0	20	theme	delivery	58:65	arg1	systems					67:73	potential anticancer drug delivery systems	32:73	potential anticancer drug delivery systems	32:73	Chitosan-dipeptide hydrogels as potential anticancer drug delivery systems.
34314799	1	21	with	self-assembly	152:164	arg1	GCS					287:289	GCS	287:289	GCS	287:289	A novel chitosan-dipeptide hydrogel was fabricated through a combination of self-assembly of 9-fluorenylmethoxycarbonyl-modified diphenylalanine (Fmoc-FF) and its electrostatic interaction with glycol chitosan (GCS).
34314799	1	21	with	self-assembly	152:164	arg1	chitosan					277:284	glycol chitosan	270:284	glycol chitosan (GCS)	270:290	A novel chitosan-dipeptide hydrogel was fabricated through a combination of self-assembly of 9-fluorenylmethoxycarbonyl-modified diphenylalanine (Fmoc-FF) and its electrostatic interaction with glycol chitosan (GCS).
34314799	9	22	theme	human	1260:1264	arg1	cells					1278:1282	A549 human lung cancer cells	1255:1282	A549 human lung cancer cells	1255:1282	The DOX-loaded hydrogel exhibited notable cytotoxicity against A549 human lung cancer cells, which suggests the newly developed hydrogel to be a promising candidate vehicle for the localized and controlled drug delivery in cancer therapy.
34314799	1	23	theme	chitosan-dipeptide	84:101	arg1	hydrogel					103:110	A novel chitosan-dipeptide hydrogel	76:110	A novel chitosan-dipeptide hydrogel	76:110	A novel chitosan-dipeptide hydrogel was fabricated through a combination of self-assembly of 9-fluorenylmethoxycarbonyl-modified diphenylalanine (Fmoc-FF) and its electrostatic interaction with glycol chitosan (GCS).
34314799	9	24	theme	promising	1337:1345	arg1	vehicle					1357:1363	a promising candidate vehicle	1335:1363	a promising candidate vehicle for the localized and controlled drug delivery	1335:1410	The DOX-loaded hydrogel exhibited notable cytotoxicity against A549 human lung cancer cells, which suggests the newly developed hydrogel to be a promising candidate vehicle for the localized and controlled drug delivery in cancer therapy.
34314799	9	25	theme	notable	1226:1232	arg1	cytotoxicity					1234:1245	notable cytotoxicity	1226:1245	notable cytotoxicity against A549 human lung cancer cells	1226:1282	The DOX-loaded hydrogel exhibited notable cytotoxicity against A549 human lung cancer cells, which suggests the newly developed hydrogel to be a promising candidate vehicle for the localized and controlled drug delivery in cancer therapy.
34314799	9	26	theme	A549	1255:1258	arg1	cells					1278:1282	A549 human lung cancer cells	1255:1282	A549 human lung cancer cells	1255:1282	The DOX-loaded hydrogel exhibited notable cytotoxicity against A549 human lung cancer cells, which suggests the newly developed hydrogel to be a promising candidate vehicle for the localized and controlled drug delivery in cancer therapy.
34314799	9	27	theme	candidate	1347:1355	arg1	vehicle					1357:1363	a promising candidate vehicle	1335:1363	a promising candidate vehicle for the localized and controlled drug delivery	1335:1410	The DOX-loaded hydrogel exhibited notable cytotoxicity against A549 human lung cancer cells, which suggests the newly developed hydrogel to be a promising candidate vehicle for the localized and controlled drug delivery in cancer therapy.
34314799	9	28	from	vehicle	1357:1363	arg1	therapy					1422:1428	cancer therapy	1415:1428	cancer therapy	1415:1428	The DOX-loaded hydrogel exhibited notable cytotoxicity against A549 human lung cancer cells, which suggests the newly developed hydrogel to be a promising candidate vehicle for the localized and controlled drug delivery in cancer therapy.
34314799	3	29	theme	highest	446:452	arg1	strength					463:470	the highest combined strength	442:470	the highest combined strength of the two interactions	442:494	The highest gel strength was observed at a Fmoc-FF mass fraction (ϕFF) of 0.85, whereby the highest combined strength of the two interactions was achieved.
34314799	0	30	theme	potential	32:40	arg1	systems					67:73	potential anticancer drug delivery systems	32:73	potential anticancer drug delivery systems	32:73	Chitosan-dipeptide hydrogels as potential anticancer drug delivery systems.
34314799	8	31	theme	DOX	1040:1042	arg1	release					1044:1050	DOX release	1040:1050	DOX release	1040:1050	Notably, DOX release was significantly accelerated as the pH of the medium decreased from 7.46 to 5.5 and 4.0, possibly due to hydrogel components' protonation.
34314799	9	32	theme	drug	1398:1401	arg1	delivery					1403:1410	the localized and controlled drug delivery	1369:1410	the localized and controlled drug delivery	1369:1410	The DOX-loaded hydrogel exhibited notable cytotoxicity against A549 human lung cancer cells, which suggests the newly developed hydrogel to be a promising candidate vehicle for the localized and controlled drug delivery in cancer therapy.
34314799	1	33	theme	electrostatic	239:251	arg1	interaction					253:263	its electrostatic interaction	235:263	its electrostatic interaction with glycol chitosan (GCS)	235:290	A novel chitosan-dipeptide hydrogel was fabricated through a combination of self-assembly of 9-fluorenylmethoxycarbonyl-modified diphenylalanine (Fmoc-FF) and its electrostatic interaction with glycol chitosan (GCS).
34314799	5	34	theme	complex	708:714	arg1	hydrogels					716:724	the complex hydrogels	704:724	the complex hydrogels	704:724	The incorporation of doxorubicin (DOX) as a cationic model drug significantly increased the stability of the complex hydrogels.
34314799	6	35	theme	gel	871:873	arg1	stability					875:883	the high gel stability	862:883	the high gel stability	862:883	DOX-loaded hydrogels exhibited slow DOX release, probably due to the drug's strong binding to Fmoc-FF via electrostatic attraction and the high gel stability.
34314799	1	36	theme	interaction	253:263	arg1	combination					137:147	a combination	135:147	a combination of self-assembly of 9-fluorenylmethoxycarbonyl-modified diphenylalanine (Fmoc-FF) and its electrostatic interaction with glycol chitosan (GCS)	135:290	A novel chitosan-dipeptide hydrogel was fabricated through a combination of self-assembly of 9-fluorenylmethoxycarbonyl-modified diphenylalanine (Fmoc-FF) and its electrostatic interaction with glycol chitosan (GCS).
34314799	0	37	theme	drug	53:56	arg1	systems					67:73	potential anticancer drug delivery systems	32:73	potential anticancer drug delivery systems	32:73	Chitosan-dipeptide hydrogels as potential anticancer drug delivery systems.
34314799	4	38	theme	buffered	569:576	arg1	solution					578:585	buffered solution	569:585	buffered solution	569:585	As the ϕFF increased above 0.6, gel stability decreased in buffered solution at pH 7.46.
34314799	5	39	theme	hydrogels	716:724	arg1	stability					691:699	the stability	687:699	the stability of the complex hydrogels	687:724	The incorporation of doxorubicin (DOX) as a cationic model drug significantly increased the stability of the complex hydrogels.
34314799	6	40	theme	high	866:869	arg1	stability					875:883	the high gel stability	862:883	the high gel stability	862:883	DOX-loaded hydrogels exhibited slow DOX release, probably due to the drug's strong binding to Fmoc-FF via electrostatic attraction and the high gel stability.
34314799	0	41	theme	anticancer	42:51	arg1	systems					67:73	potential anticancer drug delivery systems	32:73	potential anticancer drug delivery systems	32:73	Chitosan-dipeptide hydrogels as potential anticancer drug delivery systems.
34314799	9	42	theme	localized	1373:1381	arg1	delivery					1403:1410	the localized and controlled drug delivery	1369:1410	the localized and controlled drug delivery	1369:1410	The DOX-loaded hydrogel exhibited notable cytotoxicity against A549 human lung cancer cells, which suggests the newly developed hydrogel to be a promising candidate vehicle for the localized and controlled drug delivery in cancer therapy.
34314799	1	43	with	interaction	253:263	arg1	GCS					287:289	GCS	287:289	GCS	287:289	A novel chitosan-dipeptide hydrogel was fabricated through a combination of self-assembly of 9-fluorenylmethoxycarbonyl-modified diphenylalanine (Fmoc-FF) and its electrostatic interaction with glycol chitosan (GCS).
34314799	1	43	with	interaction	253:263	arg1	chitosan					277:284	glycol chitosan	270:284	glycol chitosan (GCS)	270:290	A novel chitosan-dipeptide hydrogel was fabricated through a combination of self-assembly of 9-fluorenylmethoxycarbonyl-modified diphenylalanine (Fmoc-FF) and its electrostatic interaction with glycol chitosan (GCS).
34314799	9	44	theme	lung	1266:1269	arg1	cells					1278:1282	A549 human lung cancer cells	1255:1282	A549 human lung cancer cells	1255:1282	The DOX-loaded hydrogel exhibited notable cytotoxicity against A549 human lung cancer cells, which suggests the newly developed hydrogel to be a promising candidate vehicle for the localized and controlled drug delivery in cancer therapy.
34314799	1	45	theme	glycol	270:275	arg1	GCS					287:289	GCS	287:289	GCS	287:289	A novel chitosan-dipeptide hydrogel was fabricated through a combination of self-assembly of 9-fluorenylmethoxycarbonyl-modified diphenylalanine (Fmoc-FF) and its electrostatic interaction with glycol chitosan (GCS).
34314799	1	45	theme	glycol	270:275	arg1	chitosan					277:284	glycol chitosan	270:284	glycol chitosan (GCS)	270:290	A novel chitosan-dipeptide hydrogel was fabricated through a combination of self-assembly of 9-fluorenylmethoxycarbonyl-modified diphenylalanine (Fmoc-FF) and its electrostatic interaction with glycol chitosan (GCS).
34314799	8	46	dep	7.46	1121:1124	arg1	5.5					1129:1131	5.5	1129:1131	5.5	1129:1131	Notably, DOX release was significantly accelerated as the pH of the medium decreased from 7.46 to 5.5 and 4.0, possibly due to hydrogel components' protonation.
34314799	8	46	dep	7.46	1121:1124	arg1	to					1126:1127	to	1126:1127	to	1126:1127	Notably, DOX release was significantly accelerated as the pH of the medium decreased from 7.46 to 5.5 and 4.0, possibly due to hydrogel components' protonation.
34314799	8	46	dep	7.46	1121:1124	arg1	4.0					1137:1139	4.0	1137:1139	4.0	1137:1139	Notably, DOX release was significantly accelerated as the pH of the medium decreased from 7.46 to 5.5 and 4.0, possibly due to hydrogel components' protonation.
34314799	9	47	theme	DOX-loaded	1196:1205	arg1	hydrogel					1207:1214	The DOX-loaded hydrogel	1192:1214	The DOX-loaded hydrogel	1192:1214	The DOX-loaded hydrogel exhibited notable cytotoxicity against A549 human lung cancer cells, which suggests the newly developed hydrogel to be a promising candidate vehicle for the localized and controlled drug delivery in cancer therapy.
34314799	9	48	theme	cancer	1271:1276	arg1	cells					1278:1282	A549 human lung cancer cells	1255:1282	A549 human lung cancer cells	1255:1282	The DOX-loaded hydrogel exhibited notable cytotoxicity against A549 human lung cancer cells, which suggests the newly developed hydrogel to be a promising candidate vehicle for the localized and controlled drug delivery in cancer therapy.
34314799	3	49	theme	0.85	428:431	arg1	ϕFF					420:422	ϕFF	420:422	ϕFF	420:422	The highest gel strength was observed at a Fmoc-FF mass fraction (ϕFF) of 0.85, whereby the highest combined strength of the two interactions was achieved.
34314799	3	49	theme	0.85	428:431	arg1	fraction					410:417	a Fmoc-FF mass fraction	395:417	a Fmoc-FF mass fraction (ϕFF)	395:423	The highest gel strength was observed at a Fmoc-FF mass fraction (ϕFF) of 0.85, whereby the highest combined strength of the two interactions was achieved.
34314799	9	50	theme	controlled	1387:1396	arg1	delivery					1403:1410	the localized and controlled drug delivery	1369:1410	the localized and controlled drug delivery	1369:1410	The DOX-loaded hydrogel exhibited notable cytotoxicity against A549 human lung cancer cells, which suggests the newly developed hydrogel to be a promising candidate vehicle for the localized and controlled drug delivery in cancer therapy.
34314799	3	51	theme	highest	358:364	arg1	strength					370:377	The highest gel strength	354:377	The highest gel strength	354:377	The highest gel strength was observed at a Fmoc-FF mass fraction (ϕFF) of 0.85, whereby the highest combined strength of the two interactions was achieved.
34314799	7	52	theme	excellent	917:925	arg1	features					939:946	excellent thixotropic features	917:946	excellent thixotropic features that facilitated the development of injectable self-healing drug delivery systems	917:1028	These hydrogels also exhibited excellent thixotropic features that facilitated the development of injectable self-healing drug delivery systems.
34314799	4	53	theme	gel	542:544	arg1	stability					546:554	gel stability	542:554	gel stability	542:554	As the ϕFF increased above 0.6, gel stability decreased in buffered solution at pH 7.46.
34314799	6	54	theme	electrostatic	833:845	arg1	attraction					847:856	electrostatic attraction	833:856	electrostatic attraction	833:856	DOX-loaded hydrogels exhibited slow DOX release, probably due to the drug's strong binding to Fmoc-FF via electrostatic attraction and the high gel stability.
34314799	6	55	theme	DOX	763:765	arg1	release					767:773	slow DOX release	758:773	slow DOX release	758:773	DOX-loaded hydrogels exhibited slow DOX release, probably due to the drug's strong binding to Fmoc-FF via electrostatic attraction and the high gel stability.
34314799	7	56	theme	thixotropic	927:937	arg1	features					939:946	excellent thixotropic features	917:946	excellent thixotropic features that facilitated the development of injectable self-healing drug delivery systems	917:1028	These hydrogels also exhibited excellent thixotropic features that facilitated the development of injectable self-healing drug delivery systems.
34314799	6	57	theme	slow	758:761	arg1	release					767:773	slow DOX release	758:773	slow DOX release	758:773	DOX-loaded hydrogels exhibited slow DOX release, probably due to the drug's strong binding to Fmoc-FF via electrostatic attraction and the high gel stability.
34314799	1	58	theme	self-assembly	152:164	arg1	combination					137:147	a combination	135:147	a combination of self-assembly of 9-fluorenylmethoxycarbonyl-modified diphenylalanine (Fmoc-FF) and its electrostatic interaction with glycol chitosan (GCS)	135:290	A novel chitosan-dipeptide hydrogel was fabricated through a combination of self-assembly of 9-fluorenylmethoxycarbonyl-modified diphenylalanine (Fmoc-FF) and its electrostatic interaction with glycol chitosan (GCS).
34314799	2	59	theme	Hydrogel	293:300	arg1	strength					302:309	Hydrogel strength	293:309	Hydrogel strength	293:309	Hydrogel strength and stability depended on its composition.
34314799	9	60	theme	cancer	1415:1420	arg1	therapy					1422:1428	cancer therapy	1415:1428	cancer therapy	1415:1428	The DOX-loaded hydrogel exhibited notable cytotoxicity against A549 human lung cancer cells, which suggests the newly developed hydrogel to be a promising candidate vehicle for the localized and controlled drug delivery in cancer therapy.
34314799	7	61	theme	drug	1008:1011	arg1	systems					1022:1028	injectable self-healing drug delivery systems	984:1028	injectable self-healing drug delivery systems	984:1028	These hydrogels also exhibited excellent thixotropic features that facilitated the development of injectable self-healing drug delivery systems.
34314799	1	62	theme	9-fluorenylmethoxycarbonyl-modified	169:203	arg1	Fmoc-FF					222:228	Fmoc-FF	222:228	Fmoc-FF	222:228	A novel chitosan-dipeptide hydrogel was fabricated through a combination of self-assembly of 9-fluorenylmethoxycarbonyl-modified diphenylalanine (Fmoc-FF) and its electrostatic interaction with glycol chitosan (GCS).
34314799	1	62	theme	9-fluorenylmethoxycarbonyl-modified	169:203	arg1	diphenylalanine					205:219	9-fluorenylmethoxycarbonyl-modified diphenylalanine	169:219	9-fluorenylmethoxycarbonyl-modified diphenylalanine (Fmoc-FF)	169:229	A novel chitosan-dipeptide hydrogel was fabricated through a combination of self-assembly of 9-fluorenylmethoxycarbonyl-modified diphenylalanine (Fmoc-FF) and its electrostatic interaction with glycol chitosan (GCS).
34314799	8	63	theme	hydrogel	1158:1165	arg1	protonation					1179:1189	hydrogel components' protonation	1158:1189	hydrogel components' protonation	1158:1189	Notably, DOX release was significantly accelerated as the pH of the medium decreased from 7.46 to 5.5 and 4.0, possibly due to hydrogel components' protonation.
32992840	4	0	theme	signal-regulated	774:789	arg1	kinases					791:797	extracellular signal-regulated kinases	760:797	extracellular signal-regulated kinases (ERK)	760:803	Meanwhile, atraric acid downregulated the expression of phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway to exhibit anti-inflammatory effects in LPS-stimulated RAW264.7 cells.
32992840	4	0	theme	signal-regulated	774:789	arg1	ERK					800:802	ERK	800:802	ERK	800:802	Meanwhile, atraric acid downregulated the expression of phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway to exhibit anti-inflammatory effects in LPS-stimulated RAW264.7 cells.
32992840	3	1	theme	induced	560:566	arg1	synthase					581:588	induced nitric oxide synthase	560:588	induced nitric oxide synthase	560:588	The results confirmed that atraric acid could regulate induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression in lipopolysaccharide (LPS)-stimulated RAW264.7 cells.
32992840	1	2	theme	pharmacological	268:282	arg1	activities					284:293	pharmacological activities	268:293	pharmacological activities	268:293	Lichens, composite organisms resulting from the symbiotic association between the fungi and algae, produce a variety of secondary metabolites that exhibit pharmacological activities.
32992840	0	3	theme	Mouse	99:103	arg1	Models					105:110	Mouse Models	99:110	Mouse Models	99:110	Atraric Acid Exhibits Anti-Inflammatory Effect in Lipopolysaccharide-Stimulated RAW264.7 Cells and Mouse Models.
32992840	5	4	theme	atraric	984:990	arg1	acid					992:995	atraric acid	984:995	atraric acid	984:995	Based on these results, the anti-inflammatory effect of atraric acid during LPS-induced endotoxin shock in a mouse model was confirmed.
32992840	2	5	theme	acid	397:400	arg1	activities					350:359	the anti-inflammatory activities	328:359	the anti-inflammatory activities of the secondary metabolite atraric acid produced by Heterodermia hypoleuca	328:435	This study aimed to investigate the anti-inflammatory activities of the secondary metabolite atraric acid produced by Heterodermia hypoleuca.
32992840	6	6	theme	acid	1079:1082	arg1	treated-group					1084:1096	the atraric acid treated-group	1067:1096	the atraric acid treated-group	1067:1096	In the atraric acid treated-group, cytokine production was decreased in the peritoneum and serum, and each organ damaged by LPS-stimulation was recovered.
32992840	7	7	theme	atraric	1247:1253	arg1	acid					1255:1258	atraric acid	1247:1258	atraric acid	1247:1258	These results indicate that atraric acid has an anti-inflammatory effect, which may be the underlying molecular mechanism involved in the inactivation of the ERK/NFκB signaling pathway, demonstrating its potential therapeutic value for treating inflammatory diseases.
32992840	7	8	theme	potential	1423:1431	arg1	value					1445:1449	its potential therapeutic value	1419:1449	its potential therapeutic value for treating inflammatory diseases	1419:1484	These results indicate that atraric acid has an anti-inflammatory effect, which may be the underlying molecular mechanism involved in the inactivation of the ERK/NFκB signaling pathway, demonstrating its potential therapeutic value for treating inflammatory diseases.
32992840	7	9	dep	has	1260:1262	arg1	which					1293:1297	which	1293:1297	which	1293:1297	These results indicate that atraric acid has an anti-inflammatory effect, which may be the underlying molecular mechanism involved in the inactivation of the ERK/NFκB signaling pathway, demonstrating its potential therapeutic value for treating inflammatory diseases.
32992840	7	9	dep	has	1260:1262	arg1	mechanism					1331:1339	the underlying molecular mechanism	1306:1339	the underlying molecular mechanism involved in the inactivation of the ERK/NFκB signaling pathway	1306:1402	These results indicate that atraric acid has an anti-inflammatory effect, which may be the underlying molecular mechanism involved in the inactivation of the ERK/NFκB signaling pathway, demonstrating its potential therapeutic value for treating inflammatory diseases.
32992840	3	10	theme	oxide	575:579	arg1	synthase					581:588	induced nitric oxide synthase	560:588	induced nitric oxide synthase	560:588	The results confirmed that atraric acid could regulate induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression in lipopolysaccharide (LPS)-stimulated RAW264.7 cells.
32992840	3	11	theme	enzyme	611:616	arg1	expression					618:627	induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression	493:627	induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression	493:627	The results confirmed that atraric acid could regulate induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression in lipopolysaccharide (LPS)-stimulated RAW264.7 cells.
32992840	4	12	theme	B	830:830	arg1	pathway					849:855	phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway	740:855	phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway	740:855	Meanwhile, atraric acid downregulated the expression of phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway to exhibit anti-inflammatory effects in LPS-stimulated RAW264.7 cells.
32992840	6	13	theme	atraric	1071:1077	arg1	treated-group					1084:1096	the atraric acid treated-group	1067:1096	the atraric acid treated-group	1067:1096	In the atraric acid treated-group, cytokine production was decreased in the peritoneum and serum, and each organ damaged by LPS-stimulation was recovered.
32992840	4	14	theme	nuclear	809:815	arg1	B					830:830	nuclear factor kappa B	809:830	nuclear factor kappa B (NFκB)	809:837	Meanwhile, atraric acid downregulated the expression of phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway to exhibit anti-inflammatory effects in LPS-stimulated RAW264.7 cells.
32992840	4	14	theme	nuclear	809:815	arg1	NFκB					833:836	NFκB	833:836	NFκB	833:836	Meanwhile, atraric acid downregulated the expression of phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway to exhibit anti-inflammatory effects in LPS-stimulated RAW264.7 cells.
32992840	4	15	theme	kinases	791:797	arg1	pathway					849:855	phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway	740:855	phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway	740:855	Meanwhile, atraric acid downregulated the expression of phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway to exhibit anti-inflammatory effects in LPS-stimulated RAW264.7 cells.
32992840	3	16	theme	E2	556:557	arg1	expression					618:627	induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression	493:627	induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression	493:627	The results confirmed that atraric acid could regulate induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression in lipopolysaccharide (LPS)-stimulated RAW264.7 cells.
32992840	3	17	theme	oxide	535:539	arg1	expression					618:627	induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression	493:627	induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression	493:627	The results confirmed that atraric acid could regulate induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression in lipopolysaccharide (LPS)-stimulated RAW264.7 cells.
32992840	4	18	theme	pathway	849:855	arg1	expression					726:735	the expression	722:735	the expression of phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway to exhibit anti-inflammatory effects in LPS-stimulated RAW264.7 cells	722:925	Meanwhile, atraric acid downregulated the expression of phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway to exhibit anti-inflammatory effects in LPS-stimulated RAW264.7 cells.
32992840	5	19	theme	endotoxin	1016:1024	arg1	shock					1026:1030	LPS-induced endotoxin shock	1004:1030	LPS-induced endotoxin shock in a mouse model	1004:1047	Based on these results, the anti-inflammatory effect of atraric acid during LPS-induced endotoxin shock in a mouse model was confirmed.
32992840	3	20	theme	induced	493:499	arg1	expression					618:627	induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression	493:627	induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression	493:627	The results confirmed that atraric acid could regulate induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression in lipopolysaccharide (LPS)-stimulated RAW264.7 cells.
32992840	5	21	theme	acid	992:995	arg1	effect					974:979	the anti-inflammatory effect	952:979	the anti-inflammatory effect of atraric acid during LPS-induced endotoxin shock in a mouse model	952:1047	Based on these results, the anti-inflammatory effect of atraric acid during LPS-induced endotoxin shock in a mouse model was confirmed.
32992840	3	22	theme	cyclooxygenase-2	594:609	arg1	expression					618:627	induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression	493:627	induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression	493:627	The results confirmed that atraric acid could regulate induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression in lipopolysaccharide (LPS)-stimulated RAW264.7 cells.
32992840	3	23	theme	pro-inflammatory	501:516	arg1	cytokine					518:525	pro-inflammatory cytokine	501:525	pro-inflammatory cytokine	501:525	The results confirmed that atraric acid could regulate induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression in lipopolysaccharide (LPS)-stimulated RAW264.7 cells.
32992840	5	24	from	shock	1026:1030	arg1	model					1043:1047	a mouse model	1035:1047	a mouse model	1035:1047	Based on these results, the anti-inflammatory effect of atraric acid during LPS-induced endotoxin shock in a mouse model was confirmed.
32992840	4	25	theme	kappa	824:828	arg1	B					830:830	nuclear factor kappa B	809:830	nuclear factor kappa B (NFκB)	809:837	Meanwhile, atraric acid downregulated the expression of phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway to exhibit anti-inflammatory effects in LPS-stimulated RAW264.7 cells.
32992840	4	25	theme	kappa	824:828	arg1	NFκB					833:836	NFκB	833:836	NFκB	833:836	Meanwhile, atraric acid downregulated the expression of phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway to exhibit anti-inflammatory effects in LPS-stimulated RAW264.7 cells.
32992840	0	26	theme	Atraric	0:6	arg1	Acid					8:11	Atraric Acid	0:11	Atraric Acid	0:11	Atraric Acid Exhibits Anti-Inflammatory Effect in Lipopolysaccharide-Stimulated RAW264.7 Cells and Mouse Models.
32992840	4	27	theme	factor	817:822	arg1	B					830:830	nuclear factor kappa B	809:830	nuclear factor kappa B (NFκB)	809:837	Meanwhile, atraric acid downregulated the expression of phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway to exhibit anti-inflammatory effects in LPS-stimulated RAW264.7 cells.
32992840	4	27	theme	factor	817:822	arg1	NFκB					833:836	NFκB	833:836	NFκB	833:836	Meanwhile, atraric acid downregulated the expression of phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway to exhibit anti-inflammatory effects in LPS-stimulated RAW264.7 cells.
32992840	4	28	theme	IκB	755:757	arg1	pathway					849:855	phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway	740:855	phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway	740:855	Meanwhile, atraric acid downregulated the expression of phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway to exhibit anti-inflammatory effects in LPS-stimulated RAW264.7 cells.
32992840	0	29	theme	Anti-Inflammatory	22:38	arg1	Effect					40:45	Anti-Inflammatory Effect	22:45	Anti-Inflammatory Effect	22:45	Atraric Acid Exhibits Anti-Inflammatory Effect in Lipopolysaccharide-Stimulated RAW264.7 Cells and Mouse Models.
32992840	4	30	theme	RAW264.7	912:919	arg1	cells					921:925	LPS-stimulated RAW264.7 cells	897:925	LPS-stimulated RAW264.7 cells	897:925	Meanwhile, atraric acid downregulated the expression of phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway to exhibit anti-inflammatory effects in LPS-stimulated RAW264.7 cells.
32992840	2	31	theme	anti-inflammatory	332:348	arg1	activities					350:359	the anti-inflammatory activities	328:359	the anti-inflammatory activities of the secondary metabolite atraric acid produced by Heterodermia hypoleuca	328:435	This study aimed to investigate the anti-inflammatory activities of the secondary metabolite atraric acid produced by Heterodermia hypoleuca.
32992840	4	32	theme	LPS-stimulated	897:910	arg1	cells					921:925	LPS-stimulated RAW264.7 cells	897:925	LPS-stimulated RAW264.7 cells	897:925	Meanwhile, atraric acid downregulated the expression of phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway to exhibit anti-inflammatory effects in LPS-stimulated RAW264.7 cells.
32992840	3	33	theme	nitric	528:533	arg1	oxide					535:539	nitric oxide	528:539	nitric oxide	528:539	The results confirmed that atraric acid could regulate induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression in lipopolysaccharide (LPS)-stimulated RAW264.7 cells.
32992840	6	34	dep	peritoneum	1140:1149	arg1	the					1136:1138	the	1136:1138	the	1136:1138	In the atraric acid treated-group, cytokine production was decreased in the peritoneum and serum, and each organ damaged by LPS-stimulation was recovered.
32992840	1	35	theme	composite	122:130	arg1	Lichens					113:119	Lichens	113:119	Lichens	113:119	Lichens, composite organisms resulting from the symbiotic association between the fungi and algae, produce a variety of secondary metabolites that exhibit pharmacological activities.
32992840	1	35	theme	composite	122:130	arg1	organisms					132:140	composite organisms	122:140	composite organisms resulting from the symbiotic association between the fungi and algae	122:209	Lichens, composite organisms resulting from the symbiotic association between the fungi and algae, produce a variety of secondary metabolites that exhibit pharmacological activities.
32992840	1	36	theme	symbiotic	161:169	arg1	association					171:181	the symbiotic association	157:181	the symbiotic association between the fungi and algae	157:209	Lichens, composite organisms resulting from the symbiotic association between the fungi and algae, produce a variety of secondary metabolites that exhibit pharmacological activities.
32992840	3	37	theme	atraric	465:471	arg1	acid					473:476	atraric acid	465:476	atraric acid	465:476	The results confirmed that atraric acid could regulate induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression in lipopolysaccharide (LPS)-stimulated RAW264.7 cells.
32992840	5	38	theme	LPS-induced	1004:1014	arg1	shock					1026:1030	LPS-induced endotoxin shock	1004:1030	LPS-induced endotoxin shock in a mouse model	1004:1047	Based on these results, the anti-inflammatory effect of atraric acid during LPS-induced endotoxin shock in a mouse model was confirmed.
32992840	7	39	theme	ERK/NFκB	1377:1384	arg1	pathway					1396:1402	the ERK/NFκB signaling pathway	1373:1402	the ERK/NFκB signaling pathway	1373:1402	These results indicate that atraric acid has an anti-inflammatory effect, which may be the underlying molecular mechanism involved in the inactivation of the ERK/NFκB signaling pathway, demonstrating its potential therapeutic value for treating inflammatory diseases.
32992840	3	40	theme	RAW264.7	668:675	arg1	cells					677:681	lipopolysaccharide (LPS)-stimulated RAW264.7 cells	632:681	lipopolysaccharide (LPS)-stimulated RAW264.7 cells	632:681	The results confirmed that atraric acid could regulate induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression in lipopolysaccharide (LPS)-stimulated RAW264.7 cells.
32992840	5	41	theme	anti-inflammatory	956:972	arg1	effect					974:979	the anti-inflammatory effect	952:979	the anti-inflammatory effect of atraric acid during LPS-induced endotoxin shock in a mouse model	952:1047	Based on these results, the anti-inflammatory effect of atraric acid during LPS-induced endotoxin shock in a mouse model was confirmed.
32992840	3	42	theme	cytokine	518:525	arg1	expression					618:627	induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression	493:627	induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression	493:627	The results confirmed that atraric acid could regulate induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression in lipopolysaccharide (LPS)-stimulated RAW264.7 cells.
32992840	4	43	theme	phosphorylated	740:753	arg1	pathway					849:855	phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway	740:855	phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway	740:855	Meanwhile, atraric acid downregulated the expression of phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway to exhibit anti-inflammatory effects in LPS-stimulated RAW264.7 cells.
32992840	6	44	theme	cytokine	1099:1106	arg1	production					1108:1117	cytokine production	1099:1117	cytokine production	1099:1117	In the atraric acid treated-group, cytokine production was decreased in the peritoneum and serum, and each organ damaged by LPS-stimulation was recovered.
32992840	7	45	theme	signaling	1386:1394	arg1	pathway					1396:1402	the ERK/NFκB signaling pathway	1373:1402	the ERK/NFκB signaling pathway	1373:1402	These results indicate that atraric acid has an anti-inflammatory effect, which may be the underlying molecular mechanism involved in the inactivation of the ERK/NFκB signaling pathway, demonstrating its potential therapeutic value for treating inflammatory diseases.
32992840	0	46	theme	RAW264.7	80:87	arg1	Cells					89:93	Lipopolysaccharide-Stimulated RAW264.7 Cells	50:93	Lipopolysaccharide-Stimulated RAW264.7 Cells	50:93	Atraric Acid Exhibits Anti-Inflammatory Effect in Lipopolysaccharide-Stimulated RAW264.7 Cells and Mouse Models.
32992840	1	47	dep	fungi	195:199	arg1	the					191:193	the	191:193	the	191:193	Lichens, composite organisms resulting from the symbiotic association between the fungi and algae, produce a variety of secondary metabolites that exhibit pharmacological activities.
32992840	2	48	theme	atraric	389:395	arg1	acid					397:400	the secondary metabolite atraric acid	364:400	the secondary metabolite atraric acid produced by Heterodermia hypoleuca	364:435	This study aimed to investigate the anti-inflammatory activities of the secondary metabolite atraric acid produced by Heterodermia hypoleuca.
32992840	0	49	theme	Lipopolysaccharide-Stimulated	50:78	arg1	Cells					89:93	Lipopolysaccharide-Stimulated RAW264.7 Cells	50:93	Lipopolysaccharide-Stimulated RAW264.7 Cells	50:93	Atraric Acid Exhibits Anti-Inflammatory Effect in Lipopolysaccharide-Stimulated RAW264.7 Cells and Mouse Models.
32992840	3	50	theme	-stimulated	656:666	arg1	cells					677:681	lipopolysaccharide (LPS)-stimulated RAW264.7 cells	632:681	lipopolysaccharide (LPS)-stimulated RAW264.7 cells	632:681	The results confirmed that atraric acid could regulate induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression in lipopolysaccharide (LPS)-stimulated RAW264.7 cells.
32992840	3	51	theme	nitric	568:573	arg1	synthase					581:588	induced nitric oxide synthase	560:588	induced nitric oxide synthase	560:588	The results confirmed that atraric acid could regulate induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression in lipopolysaccharide (LPS)-stimulated RAW264.7 cells.
32992840	2	52	theme	metabolite	378:387	arg1	acid					397:400	the secondary metabolite atraric acid	364:400	the secondary metabolite atraric acid produced by Heterodermia hypoleuca	364:435	This study aimed to investigate the anti-inflammatory activities of the secondary metabolite atraric acid produced by Heterodermia hypoleuca.
32992840	7	53	contain	has	1260:1262	arg1	acid					1255:1258	atraric acid	1247:1258	atraric acid	1247:1258	These results indicate that atraric acid has an anti-inflammatory effect, which may be the underlying molecular mechanism involved in the inactivation of the ERK/NFκB signaling pathway, demonstrating its potential therapeutic value for treating inflammatory diseases.
32992840	7	53	contain	has	1260:1262	arg2	effect					1285:1290	an anti-inflammatory effect	1264:1290	an anti-inflammatory effect	1264:1290	These results indicate that atraric acid has an anti-inflammatory effect, which may be the underlying molecular mechanism involved in the inactivation of the ERK/NFκB signaling pathway, demonstrating its potential therapeutic value for treating inflammatory diseases.
32992840	4	54	theme	extracellular	760:772	arg1	kinases					791:797	extracellular signal-regulated kinases	760:797	extracellular signal-regulated kinases (ERK)	760:803	Meanwhile, atraric acid downregulated the expression of phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway to exhibit anti-inflammatory effects in LPS-stimulated RAW264.7 cells.
32992840	4	54	theme	extracellular	760:772	arg1	ERK					800:802	ERK	800:802	ERK	800:802	Meanwhile, atraric acid downregulated the expression of phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway to exhibit anti-inflammatory effects in LPS-stimulated RAW264.7 cells.
32992840	2	55	theme	secondary	368:376	arg1	acid					397:400	the secondary metabolite atraric acid	364:400	the secondary metabolite atraric acid produced by Heterodermia hypoleuca	364:435	This study aimed to investigate the anti-inflammatory activities of the secondary metabolite atraric acid produced by Heterodermia hypoleuca.
32992840	5	56	theme	mouse	1037:1041	arg1	model					1043:1047	a mouse model	1035:1047	a mouse model	1035:1047	Based on these results, the anti-inflammatory effect of atraric acid during LPS-induced endotoxin shock in a mouse model was confirmed.
32992840	1	57	theme	secondary	233:241	arg1	metabolites					243:253	secondary metabolites	233:253	secondary metabolites that exhibit pharmacological activities	233:293	Lichens, composite organisms resulting from the symbiotic association between the fungi and algae, produce a variety of secondary metabolites that exhibit pharmacological activities.
32992840	4	58	theme	signaling	839:847	arg1	pathway					849:855	phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway	740:855	phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway	740:855	Meanwhile, atraric acid downregulated the expression of phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway to exhibit anti-inflammatory effects in LPS-stimulated RAW264.7 cells.
32992840	7	59	theme	inflammatory	1464:1475	arg1	diseases					1477:1484	inflammatory diseases	1464:1484	inflammatory diseases	1464:1484	These results indicate that atraric acid has an anti-inflammatory effect, which may be the underlying molecular mechanism involved in the inactivation of the ERK/NFκB signaling pathway, demonstrating its potential therapeutic value for treating inflammatory diseases.
32992840	3	60	theme	prostaglandin	542:554	arg1	E2					556:557	prostaglandin E2	542:557	prostaglandin E2	542:557	The results confirmed that atraric acid could regulate induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression in lipopolysaccharide (LPS)-stimulated RAW264.7 cells.
32992840	1	61	theme	metabolites	243:253	arg1	variety					222:228	a variety	220:228	a variety of secondary metabolites that exhibit pharmacological activities	220:293	Lichens, composite organisms resulting from the symbiotic association between the fungi and algae, produce a variety of secondary metabolites that exhibit pharmacological activities.
32992840	1	61	theme	metabolites	243:253	arg1	metabolites					243:253	secondary metabolites	233:253	secondary metabolites that exhibit pharmacological activities	233:293	Lichens, composite organisms resulting from the symbiotic association between the fungi and algae, produce a variety of secondary metabolites that exhibit pharmacological activities.
32992840	7	62	theme	pathway	1396:1402	arg1	inactivation					1357:1368	the inactivation	1353:1368	the inactivation of the ERK/NFκB signaling pathway	1353:1402	These results indicate that atraric acid has an anti-inflammatory effect, which may be the underlying molecular mechanism involved in the inactivation of the ERK/NFκB signaling pathway, demonstrating its potential therapeutic value for treating inflammatory diseases.
32992840	2	63	theme	Heterodermia	414:425	arg1	hypoleuca					427:435	Heterodermia hypoleuca	414:435	Heterodermia hypoleuca	414:435	This study aimed to investigate the anti-inflammatory activities of the secondary metabolite atraric acid produced by Heterodermia hypoleuca.
32992840	7	64	theme	therapeutic	1433:1443	arg1	value					1445:1449	its potential therapeutic value	1419:1449	its potential therapeutic value for treating inflammatory diseases	1419:1484	These results indicate that atraric acid has an anti-inflammatory effect, which may be the underlying molecular mechanism involved in the inactivation of the ERK/NFκB signaling pathway, demonstrating its potential therapeutic value for treating inflammatory diseases.
32992840	4	65	theme	atraric	695:701	arg1	Meanwhile					684:692	Meanwhile	684:692	Meanwhile	684:692	Meanwhile, atraric acid downregulated the expression of phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway to exhibit anti-inflammatory effects in LPS-stimulated RAW264.7 cells.
32992840	4	65	theme	atraric	695:701	arg1	acid					703:706	atraric acid	695:706	atraric acid	695:706	Meanwhile, atraric acid downregulated the expression of phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway to exhibit anti-inflammatory effects in LPS-stimulated RAW264.7 cells.
32992840	7	66	theme	underlying	1310:1319	arg1	which					1293:1297	which	1293:1297	which	1293:1297	These results indicate that atraric acid has an anti-inflammatory effect, which may be the underlying molecular mechanism involved in the inactivation of the ERK/NFκB signaling pathway, demonstrating its potential therapeutic value for treating inflammatory diseases.
32992840	7	66	theme	underlying	1310:1319	arg1	mechanism					1331:1339	the underlying molecular mechanism	1306:1339	the underlying molecular mechanism involved in the inactivation of the ERK/NFκB signaling pathway	1306:1402	These results indicate that atraric acid has an anti-inflammatory effect, which may be the underlying molecular mechanism involved in the inactivation of the ERK/NFκB signaling pathway, demonstrating its potential therapeutic value for treating inflammatory diseases.
32992840	7	67	theme	anti-inflammatory	1267:1283	arg1	effect					1285:1290	an anti-inflammatory effect	1264:1290	an anti-inflammatory effect	1264:1290	These results indicate that atraric acid has an anti-inflammatory effect, which may be the underlying molecular mechanism involved in the inactivation of the ERK/NFκB signaling pathway, demonstrating its potential therapeutic value for treating inflammatory diseases.
32992840	4	68	theme	anti-inflammatory	868:884	arg1	effects					886:892	anti-inflammatory effects	868:892	anti-inflammatory effects	868:892	Meanwhile, atraric acid downregulated the expression of phosphorylated IκB, extracellular signal-regulated kinases (ERK) and nuclear factor kappa B (NFκB) signaling pathway to exhibit anti-inflammatory effects in LPS-stimulated RAW264.7 cells.
32992840	3	69	theme	synthase	581:588	arg1	expression					618:627	induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression	493:627	induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression	493:627	The results confirmed that atraric acid could regulate induced pro-inflammatory cytokine, nitric oxide, prostaglandin E2, induced nitric oxide synthase and cyclooxygenase-2 enzyme expression in lipopolysaccharide (LPS)-stimulated RAW264.7 cells.
32992840	7	70	theme	molecular	1321:1329	arg1	which					1293:1297	which	1293:1297	which	1293:1297	These results indicate that atraric acid has an anti-inflammatory effect, which may be the underlying molecular mechanism involved in the inactivation of the ERK/NFκB signaling pathway, demonstrating its potential therapeutic value for treating inflammatory diseases.
32992840	7	70	theme	molecular	1321:1329	arg1	mechanism					1331:1339	the underlying molecular mechanism	1306:1339	the underlying molecular mechanism involved in the inactivation of the ERK/NFκB signaling pathway	1306:1402	These results indicate that atraric acid has an anti-inflammatory effect, which may be the underlying molecular mechanism involved in the inactivation of the ERK/NFκB signaling pathway, demonstrating its potential therapeutic value for treating inflammatory diseases.
34383247	5	0	theme	endolysin	573:581	arg1	LysSAP33					583:590	An endolysin LysSAP33	570:590	An endolysin LysSAP33 encoded on bacteriophage SAP33	570:621	An endolysin LysSAP33 encoded on bacteriophage SAP33 shared low homology with LysK-like endolysin in sequence by 41% and domain composition (CHAP-unknown CBD).
34383247	5	1	theme	CHAP-unknown	711:722	arg1	composition					698:708	domain composition	691:708	domain composition (CHAP-unknown CBD)	691:727	An endolysin LysSAP33 encoded on bacteriophage SAP33 shared low homology with LysK-like endolysin in sequence by 41% and domain composition (CHAP-unknown CBD).
34383247	5	1	theme	CHAP-unknown	711:722	arg1	CBD					724:726	CHAP-unknown CBD	711:726	CHAP-unknown CBD	711:726	An endolysin LysSAP33 encoded on bacteriophage SAP33 shared low homology with LysK-like endolysin in sequence by 41% and domain composition (CHAP-unknown CBD).
34383247	5	2	theme	domain	691:696	arg1	composition					698:708	domain composition	691:708	domain composition (CHAP-unknown CBD)	691:727	An endolysin LysSAP33 encoded on bacteriophage SAP33 shared low homology with LysK-like endolysin in sequence by 41% and domain composition (CHAP-unknown CBD).
34383247	5	2	theme	domain	691:696	arg1	CBD					724:726	CHAP-unknown CBD	711:726	CHAP-unknown CBD	711:726	An endolysin LysSAP33 encoded on bacteriophage SAP33 shared low homology with LysK-like endolysin in sequence by 41% and domain composition (CHAP-unknown CBD).
34383247	7	3	theme	lytic	977:981	arg1	activity					983:990	lytic activity	977:990	lytic activity	977:990	The deletion of LysSAP33_CBD at the C-terminal region resulted in a significant decrease in lytic activity and efficacy.
34383247	10	4	theme	helpful	1246:1252	arg1	tool					1254:1257	a helpful tool	1244:1257	a helpful tool to develop our understanding of staphylococcal endolysins not belonging to LysK-like endolysins and a potential biocontrol agent against biofilms	1244:1403	This study could be a helpful tool to develop our understanding of staphylococcal endolysins not belonging to LysK-like endolysins and a potential biocontrol agent against biofilms.
34383247	10	4	theme	helpful	1246:1252	arg1	study					1229:1233	This study	1224:1233	This study	1224:1233	This study could be a helpful tool to develop our understanding of staphylococcal endolysins not belonging to LysK-like endolysins and a potential biocontrol agent against biofilms.
34383247	2	5	from	endolysins	223:232	arg1	phages					252:257	staphylococcal phages	237:257	staphylococcal phages	237:257	Many endolysins on staphylococcal phages have been identified and applied extensively against Staphylococcus spp.
34383247	9	6	theme	LysK-like	1203:1211	arg1	endolysin					1213:1221	LysK-like endolysin	1203:1221	LysK-like endolysin	1203:1221	In addition, it showed a higher activity against biofilms than LysK-like endolysin.
34383247	8	7	theme	lytic	1061:1065	arg1	activity					1067:1074	its lytic activity	1057:1074	its lytic activity	1057:1074	Compared to LysK-like endolysin, LysSAP33 retained its lytic activity in a broader range of temperature, pH, and NaCl concentrations.
34383247	5	8	theme	LysK-like	648:656	arg1	endolysin					658:666	LysK-like endolysin	648:666	LysK-like endolysin in sequence by 41%	648:685	An endolysin LysSAP33 encoded on bacteriophage SAP33 shared low homology with LysK-like endolysin in sequence by 41% and domain composition (CHAP-unknown CBD).
34383247	6	9	theme	CBD	816:818	arg1	domain					820:825	the CBD domain	812:825	the CBD domain (157-251 aa)	812:838	A green fluorescence assay using a fusion protein for LysSAP33_CBD indicated that the CBD domain (157-251 aa) was bound to the peptidoglycan of S. aureus.
34383247	6	9	theme	CBD	816:818	arg1	aa					836:837	157-251 aa	828:837	157-251 aa	828:837	A green fluorescence assay using a fusion protein for LysSAP33_CBD indicated that the CBD domain (157-251 aa) was bound to the peptidoglycan of S. aureus.
34383247	7	10	theme	LysSAP33_CBD	901:912	arg1	deletion					889:896	The deletion	885:896	The deletion of LysSAP33_CBD at the C-terminal region	885:937	The deletion of LysSAP33_CBD at the C-terminal region resulted in a significant decrease in lytic activity and efficacy.
34383247	6	11	theme	fluorescence	738:749	arg1	assay					751:755	A green fluorescence assay	730:755	A green fluorescence assay using a fusion protein for LysSAP33_CBD	730:795	A green fluorescence assay using a fusion protein for LysSAP33_CBD indicated that the CBD domain (157-251 aa) was bound to the peptidoglycan of S. aureus.
34383247	1	12	theme	alternative	185:195	arg1	agent					211:215	an alternative antimicrobial agent	182:215	an alternative antimicrobial agent	182:215	Endolysin, a peptidoglycan hydrolase derived from bacteriophage, has been suggested as an alternative antimicrobial agent.
34383247	1	12	theme	alternative	185:195	arg1	Endolysin					95:103	Endolysin	95:103	Endolysin	95:103	Endolysin, a peptidoglycan hydrolase derived from bacteriophage, has been suggested as an alternative antimicrobial agent.
34383247	8	13	theme	LysK-like	1018:1026	arg1	endolysin					1028:1036	LysK-like endolysin	1018:1036	LysK-like endolysin	1018:1036	Compared to LysK-like endolysin, LysSAP33 retained its lytic activity in a broader range of temperature, pH, and NaCl concentrations.
34383247	2	14	theme	Staphylococcus	312:325	arg1	spp					327:329	Staphylococcus spp	312:329	Staphylococcus spp	312:329	Many endolysins on staphylococcal phages have been identified and applied extensively against Staphylococcus spp.
34383247	2	15	theme	staphylococcal	237:250	arg1	phages					252:257	staphylococcal phages	237:257	staphylococcal phages	237:257	Many endolysins on staphylococcal phages have been identified and applied extensively against Staphylococcus spp.
34383247	1	16	theme	antimicrobial	197:209	arg1	agent					211:215	an alternative antimicrobial agent	182:215	an alternative antimicrobial agent	182:215	Endolysin, a peptidoglycan hydrolase derived from bacteriophage, has been suggested as an alternative antimicrobial agent.
34383247	1	16	theme	antimicrobial	197:209	arg1	Endolysin					95:103	Endolysin	95:103	Endolysin	95:103	Endolysin, a peptidoglycan hydrolase derived from bacteriophage, has been suggested as an alternative antimicrobial agent.
34383247	8	17	theme	broader	1081:1087	arg1	range					1089:1093	a broader range	1079:1093	a broader range of temperature, pH, and NaCl concentrations	1079:1137	Compared to LysK-like endolysin, LysSAP33 retained its lytic activity in a broader range of temperature, pH, and NaCl concentrations.
34383247	7	18	theme	significant	953:963	arg1	decrease					965:972	a significant decrease	951:972	a significant decrease in lytic activity and efficacy	951:1003	The deletion of LysSAP33_CBD at the C-terminal region resulted in a significant decrease in lytic activity and efficacy.
34383247	3	19	theme	identified	431:440	arg1	endolysins					442:451	the identified endolysins	427:451	the identified endolysins	427:451	Among them, LysK-like endolysin, a well-studied staphylococcal endolysin, accounts for most of the identified endolysins.
34383247	5	20	theme	low	630:632	arg1	homology					634:641	low homology	630:641	low homology with LysK-like endolysin in sequence by 41% and domain composition (CHAP-unknown CBD)	630:727	An endolysin LysSAP33 encoded on bacteriophage SAP33 shared low homology with LysK-like endolysin in sequence by 41% and domain composition (CHAP-unknown CBD).
34383247	1	21	theme	peptidoglycan	108:120	arg1	Endolysin					95:103	Endolysin	95:103	Endolysin	95:103	Endolysin, a peptidoglycan hydrolase derived from bacteriophage, has been suggested as an alternative antimicrobial agent.
34383247	1	21	theme	peptidoglycan	108:120	arg1	hydrolase					122:130	a peptidoglycan hydrolase	106:130	a peptidoglycan hydrolase derived from bacteriophage	106:157	Endolysin, a peptidoglycan hydrolase derived from bacteriophage, has been suggested as an alternative antimicrobial agent.
34383247	0	22	theme	endolysin	35:43	arg1	LysSAP33					45:52	staphylococcal endolysin LysSAP33	20:52	staphylococcal endolysin LysSAP33	20:52	Characterization of staphylococcal endolysin LysSAP33 possessing untypical domain composition.
34383247	5	23	with	homology	634:641	arg1	endolysin					658:666	LysK-like endolysin	648:666	LysK-like endolysin in sequence by 41%	648:685	An endolysin LysSAP33 encoded on bacteriophage SAP33 shared low homology with LysK-like endolysin in sequence by 41% and domain composition (CHAP-unknown CBD).
34383247	5	23	with	homology	634:641	arg1	composition					698:708	domain composition	691:708	domain composition (CHAP-unknown CBD)	691:727	An endolysin LysSAP33 encoded on bacteriophage SAP33 shared low homology with LysK-like endolysin in sequence by 41% and domain composition (CHAP-unknown CBD).
34383247	5	23	with	homology	634:641	arg1	CBD					724:726	CHAP-unknown CBD	711:726	CHAP-unknown CBD	711:726	An endolysin LysSAP33 encoded on bacteriophage SAP33 shared low homology with LysK-like endolysin in sequence by 41% and domain composition (CHAP-unknown CBD).
34383247	9	24	theme	higher	1165:1170	arg1	activity					1172:1179	a higher activity	1163:1179	a higher activity against biofilms than LysK-like endolysin	1163:1221	In addition, it showed a higher activity against biofilms than LysK-like endolysin.
34383247	0	25	theme	staphylococcal	20:33	arg1	LysSAP33					45:52	staphylococcal endolysin LysSAP33	20:52	staphylococcal endolysin LysSAP33	20:52	Characterization of staphylococcal endolysin LysSAP33 possessing untypical domain composition.
34383247	7	26	from	region	932:937	arg1	deletion					889:896	The deletion	885:896	The deletion of LysSAP33_CBD at the C-terminal region	885:937	The deletion of LysSAP33_CBD at the C-terminal region resulted in a significant decrease in lytic activity and efficacy.
34383247	10	27	theme	endolysins	1306:1315	arg1	understanding					1274:1286	our understanding	1270:1286	our understanding of staphylococcal endolysins not belonging to LysK-like endolysins	1270:1353	This study could be a helpful tool to develop our understanding of staphylococcal endolysins not belonging to LysK-like endolysins and a potential biocontrol agent against biofilms.
34383247	10	27	theme	endolysins	1306:1315	arg1	agent					1382:1386	a potential biocontrol agent	1359:1386	a potential biocontrol agent against biofilms	1359:1403	This study could be a helpful tool to develop our understanding of staphylococcal endolysins not belonging to LysK-like endolysins and a potential biocontrol agent against biofilms.
34383247	7	28	from	decrease	965:972	arg1	efficacy					996:1003	efficacy	996:1003	efficacy	996:1003	The deletion of LysSAP33_CBD at the C-terminal region resulted in a significant decrease in lytic activity and efficacy.
34383247	7	28	from	decrease	965:972	arg1	activity					983:990	lytic activity	977:990	lytic activity	977:990	The deletion of LysSAP33_CBD at the C-terminal region resulted in a significant decrease in lytic activity and efficacy.
34383247	6	29	theme	aureus	877:882	arg1	peptidoglycan					857:869	the peptidoglycan	853:869	the peptidoglycan of S. aureus	853:882	A green fluorescence assay using a fusion protein for LysSAP33_CBD indicated that the CBD domain (157-251 aa) was bound to the peptidoglycan of S. aureus.
34383247	10	30	theme	staphylococcal	1291:1304	arg1	endolysins					1306:1315	staphylococcal endolysins	1291:1315	staphylococcal endolysins not belonging to LysK-like endolysins	1291:1353	This study could be a helpful tool to develop our understanding of staphylococcal endolysins not belonging to LysK-like endolysins and a potential biocontrol agent against biofilms.
34383247	0	31	theme	LysSAP33	45:52	arg1	Characterization					0:15	Characterization	0:15	Characterization of staphylococcal endolysin LysSAP33	0:52	Characterization of staphylococcal endolysin LysSAP33 possessing untypical domain composition.
34383247	5	32	from	composition	698:708	arg1	sequence					671:678	sequence	671:678	sequence	671:678	An endolysin LysSAP33 encoded on bacteriophage SAP33 shared low homology with LysK-like endolysin in sequence by 41% and domain composition (CHAP-unknown CBD).
34383247	8	33	theme	temperature	1098:1108	arg1	range					1089:1093	a broader range	1079:1093	a broader range of temperature, pH, and NaCl concentrations	1079:1137	Compared to LysK-like endolysin, LysSAP33 retained its lytic activity in a broader range of temperature, pH, and NaCl concentrations.
34383247	4	34	theme	LysKunlike	507:516	arg1	endolysin					518:526	LysKunlike endolysin	507:526	LysKunlike endolysin	507:526	However, relatively little interest has been paid to LysKunlike endolysin and a few of them has been characterized.
34383247	0	35	theme	domain	75:80	arg1	composition					82:92	untypical domain composition	65:92	untypical domain composition	65:92	Characterization of staphylococcal endolysin LysSAP33 possessing untypical domain composition.
34383247	3	36	theme	LysK-like	344:352	arg1	endolysin					395:403	a well-studied staphylococcal endolysin	365:403	a well-studied staphylococcal endolysin	365:403	Among them, LysK-like endolysin, a well-studied staphylococcal endolysin, accounts for most of the identified endolysins.
34383247	3	36	theme	LysK-like	344:352	arg1	endolysin					354:362	LysK-like endolysin	344:362	LysK-like endolysin	344:362	Among them, LysK-like endolysin, a well-studied staphylococcal endolysin, accounts for most of the identified endolysins.
34383247	8	37	theme	concentrations	1124:1137	arg1	range					1089:1093	a broader range	1079:1093	a broader range of temperature, pH, and NaCl concentrations	1079:1137	Compared to LysK-like endolysin, LysSAP33 retained its lytic activity in a broader range of temperature, pH, and NaCl concentrations.
34383247	0	38	theme	untypical	65:73	arg1	composition					82:92	untypical domain composition	65:92	untypical domain composition	65:92	Characterization of staphylococcal endolysin LysSAP33 possessing untypical domain composition.
34383247	1	39	attach	derived	132:138	arg2	hydrolase					122:130	a peptidoglycan hydrolase	106:130	a peptidoglycan hydrolase derived from bacteriophage	106:157	Endolysin, a peptidoglycan hydrolase derived from bacteriophage, has been suggested as an alternative antimicrobial agent.
34383247	1	39	attach	derived	132:138	arg1	bacteriophage					145:157	bacteriophage	145:157	bacteriophage	145:157	Endolysin, a peptidoglycan hydrolase derived from bacteriophage, has been suggested as an alternative antimicrobial agent.
34383247	1	39	attach	derived	132:138	arg2	Endolysin					95:103	Endolysin	95:103	Endolysin	95:103	Endolysin, a peptidoglycan hydrolase derived from bacteriophage, has been suggested as an alternative antimicrobial agent.
34383247	8	40	theme	pH	1111:1112	arg1	range					1089:1093	a broader range	1079:1093	a broader range of temperature, pH, and NaCl concentrations	1079:1137	Compared to LysK-like endolysin, LysSAP33 retained its lytic activity in a broader range of temperature, pH, and NaCl concentrations.
34383247	4	41	theme	little	474:479	arg1	interest					481:488	relatively little interest	463:488	relatively little interest	463:488	However, relatively little interest has been paid to LysKunlike endolysin and a few of them has been characterized.
34383247	10	42	theme	LysK-like	1334:1342	arg1	endolysins					1344:1353	LysK-like endolysins	1334:1353	LysK-like endolysins	1334:1353	This study could be a helpful tool to develop our understanding of staphylococcal endolysins not belonging to LysK-like endolysins and a potential biocontrol agent against biofilms.
34383247	7	43	theme	C-terminal	921:930	arg1	region					932:937	the C-terminal region	917:937	the C-terminal region	917:937	The deletion of LysSAP33_CBD at the C-terminal region resulted in a significant decrease in lytic activity and efficacy.
34383247	10	44	theme	biocontrol	1371:1380	arg1	agent					1382:1386	a potential biocontrol agent	1359:1386	a potential biocontrol agent against biofilms	1359:1403	This study could be a helpful tool to develop our understanding of staphylococcal endolysins not belonging to LysK-like endolysins and a potential biocontrol agent against biofilms.
34383247	8	45	theme	NaCl	1119:1122	arg1	concentrations					1124:1137	NaCl concentrations	1119:1137	NaCl concentrations	1119:1137	Compared to LysK-like endolysin, LysSAP33 retained its lytic activity in a broader range of temperature, pH, and NaCl concentrations.
34383247	5	46	theme	bacteriophage	603:615	arg1	SAP33					617:621	bacteriophage SAP33	603:621	bacteriophage SAP33	603:621	An endolysin LysSAP33 encoded on bacteriophage SAP33 shared low homology with LysK-like endolysin in sequence by 41% and domain composition (CHAP-unknown CBD).
34383247	6	47	theme	green	732:736	arg1	assay					751:755	A green fluorescence assay	730:755	A green fluorescence assay using a fusion protein for LysSAP33_CBD	730:795	A green fluorescence assay using a fusion protein for LysSAP33_CBD indicated that the CBD domain (157-251 aa) was bound to the peptidoglycan of S. aureus.
34383247	3	48	theme	well-studied	367:378	arg1	endolysin					395:403	a well-studied staphylococcal endolysin	365:403	a well-studied staphylococcal endolysin	365:403	Among them, LysK-like endolysin, a well-studied staphylococcal endolysin, accounts for most of the identified endolysins.
34383247	3	48	theme	well-studied	367:378	arg1	endolysin					354:362	LysK-like endolysin	344:362	LysK-like endolysin	344:362	Among them, LysK-like endolysin, a well-studied staphylococcal endolysin, accounts for most of the identified endolysins.
34383247	2	49	theme	Many	218:221	arg1	endolysins					223:232	Many endolysins	218:232	Many endolysins on staphylococcal phages	218:257	Many endolysins on staphylococcal phages have been identified and applied extensively against Staphylococcus spp.
34383247	6	50	theme	fusion	765:770	arg1	protein					772:778	a fusion protein	763:778	a fusion protein for LysSAP33_CBD	763:795	A green fluorescence assay using a fusion protein for LysSAP33_CBD indicated that the CBD domain (157-251 aa) was bound to the peptidoglycan of S. aureus.
34383247	5	51	from	endolysin	658:666	arg1	sequence					671:678	sequence	671:678	sequence	671:678	An endolysin LysSAP33 encoded on bacteriophage SAP33 shared low homology with LysK-like endolysin in sequence by 41% and domain composition (CHAP-unknown CBD).
34383247	3	52	theme	staphylococcal	380:393	arg1	endolysin					395:403	a well-studied staphylococcal endolysin	365:403	a well-studied staphylococcal endolysin	365:403	Among them, LysK-like endolysin, a well-studied staphylococcal endolysin, accounts for most of the identified endolysins.
34383247	3	52	theme	staphylococcal	380:393	arg1	endolysin					354:362	LysK-like endolysin	344:362	LysK-like endolysin	344:362	Among them, LysK-like endolysin, a well-studied staphylococcal endolysin, accounts for most of the identified endolysins.
34383247	4	53	theme	them	541:544	arg1	few					534:536	few	534:536	few	534:536	However, relatively little interest has been paid to LysKunlike endolysin and a few of them has been characterized.
34383247	10	54	theme	potential	1361:1369	arg1	agent					1382:1386	a potential biocontrol agent	1359:1386	a potential biocontrol agent against biofilms	1359:1403	This study could be a helpful tool to develop our understanding of staphylococcal endolysins not belonging to LysK-like endolysins and a potential biocontrol agent against biofilms.
33068039	5	0	with	interactions	940:951	arg1	TfR					958:960	TfR	958:960	TfR	958:960	In pursuits for finding therapeutic solutions for the New World arenaviruses, and to gain a greater understanding of MGP1 interactions with TfR, it is crucial to understand the structure-sequence relationship driving the interface formation.
33068039	9	1	theme	blood-brain	1701:1711	arg1	barrier					1713:1719	the blood-brain barrier	1697:1719	the blood-brain barrier crossing	1697:1728	The engineered variants can be used to probe cellular internalization or the blood-brain barrier crossing to achieve greater understanding of TfR mediated internalization.
33068039	5	2	theme	World	876:880	arg1	arenaviruses					882:893	the New World arenaviruses	868:893	the New World arenaviruses	868:893	In pursuits for finding therapeutic solutions for the New World arenaviruses, and to gain a greater understanding of MGP1 interactions with TfR, it is crucial to understand the structure-sequence relationship driving the interface formation.
33068039	0	3	theme	transferrin	74:84	arg1	receptor					86:93	human transferrin receptor	68:93	human transferrin receptor	68:93	Tuning the binding interface between Machupo virus glycoprotein and human transferrin receptor.
33068039	8	4	theme	further	1590:1596	arg1	substitutions					1609:1621	further amino acid substitutions	1590:1621	further amino acid substitutions	1590:1621	We show that the optimized MGP1 variants can bind more strongly to TfR than the native MGP1, and there is an MGP1 sequence that retains binding in the absence of glycosylation, but with the addition of further amino acid substitutions.
33068039	6	5	theme	yeast	1082:1086	arg1	surface					1088:1094	yeast surface	1082:1094	yeast surface	1082:1094	By displaying MGP1 on yeast surface we have examined the contributions of individual residues to the binding of solubilized ectodomain of TfR.
33068039	1	6	theme	human	153:157	arg1	cells					159:163	human cells	153:163	human cells	153:163	Machupo virus, known to cause hemorrhagic fevers, enters human cells via binding with its envelope glycoprotein to transferrin receptor 1 (TfR).
33068039	9	7	theme	greater	1741:1747	arg1	understanding					1749:1761	greater understanding	1741:1761	greater understanding of TfR mediated internalization	1741:1793	The engineered variants can be used to probe cellular internalization or the blood-brain barrier crossing to achieve greater understanding of TfR mediated internalization.
33068039	8	8	theme	optimized	1405:1413	arg1	variants					1420:1427	the optimized MGP1 variants	1401:1427	the optimized MGP1 variants	1401:1427	We show that the optimized MGP1 variants can bind more strongly to TfR than the native MGP1, and there is an MGP1 sequence that retains binding in the absence of glycosylation, but with the addition of further amino acid substitutions.
33068039	3	9	from	Study	511:515	arg1	complex					589:595	complex	589:595	complex with TfR	589:604	Study of the experimental structure of Machupo virus glycoprotein 1 (MGP1) in complex with TfR and glycoprotein sequence homology has identified some residues at the interface that influence binding.
33068039	3	9	from	Study	511:515	arg1	homology					632:639	glycoprotein sequence homology	610:639	glycoprotein sequence homology	610:639	Study of the experimental structure of Machupo virus glycoprotein 1 (MGP1) in complex with TfR and glycoprotein sequence homology has identified some residues at the interface that influence binding.
33068039	5	10	theme	MGP1	935:938	arg1	interactions					940:951	MGP1 interactions	935:951	MGP1 interactions with TfR	935:960	In pursuits for finding therapeutic solutions for the New World arenaviruses, and to gain a greater understanding of MGP1 interactions with TfR, it is crucial to understand the structure-sequence relationship driving the interface formation.
33068039	3	11	theme	glycoprotein	564:575	arg1	structure					537:545	the experimental structure	520:545	the experimental structure of Machupo virus glycoprotein 1 (MGP1)	520:584	Study of the experimental structure of Machupo virus glycoprotein 1 (MGP1) in complex with TfR and glycoprotein sequence homology has identified some residues at the interface that influence binding.
33068039	8	12	theme	amino	1598:1602	arg1	substitutions					1609:1621	further amino acid substitutions	1590:1621	further amino acid substitutions	1590:1621	We show that the optimized MGP1 variants can bind more strongly to TfR than the native MGP1, and there is an MGP1 sequence that retains binding in the absence of glycosylation, but with the addition of further amino acid substitutions.
33068039	6	13	theme	individual	1134:1143	arg1	residues					1145:1152	individual residues	1134:1152	individual residues	1134:1152	By displaying MGP1 on yeast surface we have examined the contributions of individual residues to the binding of solubilized ectodomain of TfR.
33068039	3	14	theme	Machupo	550:556	arg1	glycoprotein					564:575	Machupo virus glycoprotein 1	550:577	Machupo virus glycoprotein 1 (MGP1)	550:584	Study of the experimental structure of Machupo virus glycoprotein 1 (MGP1) in complex with TfR and glycoprotein sequence homology has identified some residues at the interface that influence binding.
33068039	3	14	theme	Machupo	550:556	arg1	MGP1					580:583	MGP1	580:583	MGP1	580:583	Study of the experimental structure of Machupo virus glycoprotein 1 (MGP1) in complex with TfR and glycoprotein sequence homology has identified some residues at the interface that influence binding.
33068039	7	15	theme	hot	1230:1232	arg1	spot					1234:1237	MGP1 binding hot spot	1217:1237	MGP1 binding hot spot residues	1217:1246	We identified MGP1 binding hot spot residues, assessed the importance of posttranslational N-glycan modifications, and used a selection with random mutagenesis for affinity maturation.
33068039	2	16	theme	receptor	256:263	arg1	system					345:350	a molecular system	333:350	a molecular system to ferry therapeutics across the cellular membranes and through the impenetrable blood-brain barrier that effectively blocks any such delivery into the brain	333:508	Similarly, the receptor interactions have been explored in biotechnological applications as a molecular system to ferry therapeutics across the cellular membranes and through the impenetrable blood-brain barrier that effectively blocks any such delivery into the brain.
33068039	2	16	theme	receptor	256:263	arg1	interactions					265:276	the receptor interactions	252:276	the receptor interactions	252:276	Similarly, the receptor interactions have been explored in biotechnological applications as a molecular system to ferry therapeutics across the cellular membranes and through the impenetrable blood-brain barrier that effectively blocks any such delivery into the brain.
33068039	2	17	theme	biotechnological	300:315	arg1	applications					317:328	biotechnological applications	300:328	biotechnological applications	300:328	Similarly, the receptor interactions have been explored in biotechnological applications as a molecular system to ferry therapeutics across the cellular membranes and through the impenetrable blood-brain barrier that effectively blocks any such delivery into the brain.
33068039	9	18	theme	engineered	1628:1637	arg1	variants					1639:1646	The engineered variants	1624:1646	The engineered variants	1624:1646	The engineered variants can be used to probe cellular internalization or the blood-brain barrier crossing to achieve greater understanding of TfR mediated internalization.
33068039	7	19	theme	modifications	1303:1315	arg1	importance					1262:1271	the importance	1258:1271	the importance of posttranslational N-glycan modifications	1258:1315	We identified MGP1 binding hot spot residues, assessed the importance of posttranslational N-glycan modifications, and used a selection with random mutagenesis for affinity maturation.
33068039	3	20	theme	experimental	524:535	arg1	structure					537:545	the experimental structure	520:545	the experimental structure of Machupo virus glycoprotein 1 (MGP1)	520:584	Study of the experimental structure of Machupo virus glycoprotein 1 (MGP1) in complex with TfR and glycoprotein sequence homology has identified some residues at the interface that influence binding.
33068039	5	21	from	crucial	969:975	arg1	pursuits					821:828	pursuits	821:828	pursuits for finding therapeutic solutions for the New World arenaviruses, and to gain a greater understanding of MGP1 interactions with TfR	821:960	In pursuits for finding therapeutic solutions for the New World arenaviruses, and to gain a greater understanding of MGP1 interactions with TfR, it is crucial to understand the structure-sequence relationship driving the interface formation.
33068039	2	22	theme	cellular	385:392	arg1	membranes					394:402	the cellular membranes	381:402	the cellular membranes	381:402	Similarly, the receptor interactions have been explored in biotechnological applications as a molecular system to ferry therapeutics across the cellular membranes and through the impenetrable blood-brain barrier that effectively blocks any such delivery into the brain.
33068039	3	23	with	complex	589:595	arg1	TfR					602:604	TfR	602:604	TfR	602:604	Study of the experimental structure of Machupo virus glycoprotein 1 (MGP1) in complex with TfR and glycoprotein sequence homology has identified some residues at the interface that influence binding.
33068039	2	24	theme	such	481:484	arg1	delivery					486:493	any such delivery	477:493	any such delivery into the brain	477:508	Similarly, the receptor interactions have been explored in biotechnological applications as a molecular system to ferry therapeutics across the cellular membranes and through the impenetrable blood-brain barrier that effectively blocks any such delivery into the brain.
33068039	3	25	theme	structure	537:545	arg1	Study					511:515	Study	511:515	Study of the experimental structure of Machupo virus glycoprotein 1 (MGP1) in complex with TfR and glycoprotein sequence homology	511:639	Study of the experimental structure of Machupo virus glycoprotein 1 (MGP1) in complex with TfR and glycoprotein sequence homology has identified some residues at the interface that influence binding.
33068039	5	26	theme	therapeutic	842:852	arg1	solutions					854:862	therapeutic solutions	842:862	therapeutic solutions for the New World arenaviruses	842:893	In pursuits for finding therapeutic solutions for the New World arenaviruses, and to gain a greater understanding of MGP1 interactions with TfR, it is crucial to understand the structure-sequence relationship driving the interface formation.
33068039	9	27	theme	mediated	1770:1777	arg1	internalization					1779:1793	TfR mediated internalization	1766:1793	TfR mediated internalization	1766:1793	The engineered variants can be used to probe cellular internalization or the blood-brain barrier crossing to achieve greater understanding of TfR mediated internalization.
33068039	8	28	theme	MGP1	1497:1500	arg1	sequence					1502:1509	an MGP1 sequence	1494:1509	an MGP1 sequence that retains binding in the absence of glycosylation, but with the addition of further amino acid substitutions	1494:1621	We show that the optimized MGP1 variants can bind more strongly to TfR than the native MGP1, and there is an MGP1 sequence that retains binding in the absence of glycosylation, but with the addition of further amino acid substitutions.
33068039	7	29	with	selection	1329:1337	arg1	mutagenesis					1351:1361	random mutagenesis	1344:1361	random mutagenesis	1344:1361	We identified MGP1 binding hot spot residues, assessed the importance of posttranslational N-glycan modifications, and used a selection with random mutagenesis for affinity maturation.
33068039	6	30	theme	residues	1145:1152	arg1	contributions					1117:1129	the contributions	1113:1129	the contributions of individual residues to the binding of solubilized ectodomain of TfR	1113:1200	By displaying MGP1 on yeast surface we have examined the contributions of individual residues to the binding of solubilized ectodomain of TfR.
33068039	9	31	theme	internalization	1779:1793	arg1	understanding					1749:1761	greater understanding	1741:1761	greater understanding of TfR mediated internalization	1741:1793	The engineered variants can be used to probe cellular internalization or the blood-brain barrier crossing to achieve greater understanding of TfR mediated internalization.
33068039	9	32	theme	cellular	1669:1676	arg1	internalization					1678:1692	cellular internalization	1669:1692	cellular internalization	1669:1692	The engineered variants can be used to probe cellular internalization or the blood-brain barrier crossing to achieve greater understanding of TfR mediated internalization.
33068039	1	33	gly	glycoprotein	195:206	arg1	glycoprotein					195:206	its envelope glycoprotein	182:206	its envelope glycoprotein	182:206	Machupo virus, known to cause hemorrhagic fevers, enters human cells via binding with its envelope glycoprotein to transferrin receptor 1 (TfR).
33068039	3	34	from	interface	677:685	arg1	residues					661:668	some residues	656:668	some residues at the interface that influence binding	656:708	Study of the experimental structure of Machupo virus glycoprotein 1 (MGP1) in complex with TfR and glycoprotein sequence homology has identified some residues at the interface that influence binding.
33068039	1	35	theme	Machupo	96:102	arg1	virus					104:108	Machupo virus	96:108	Machupo virus	96:108	Machupo virus, known to cause hemorrhagic fevers, enters human cells via binding with its envelope glycoprotein to transferrin receptor 1 (TfR).
33068039	2	36	theme	blood-brain	433:443	arg1	barrier					445:451	the impenetrable blood-brain barrier	416:451	the impenetrable blood-brain barrier that effectively blocks any such delivery into the brain	416:508	Similarly, the receptor interactions have been explored in biotechnological applications as a molecular system to ferry therapeutics across the cellular membranes and through the impenetrable blood-brain barrier that effectively blocks any such delivery into the brain.
33068039	7	37	theme	affinity	1367:1374	arg1	maturation					1376:1385	affinity maturation	1367:1385	affinity maturation	1367:1385	We identified MGP1 binding hot spot residues, assessed the importance of posttranslational N-glycan modifications, and used a selection with random mutagenesis for affinity maturation.
33068039	3	38	theme	virus	558:562	arg1	glycoprotein					564:575	Machupo virus glycoprotein 1	550:577	Machupo virus glycoprotein 1 (MGP1)	550:584	Study of the experimental structure of Machupo virus glycoprotein 1 (MGP1) in complex with TfR and glycoprotein sequence homology has identified some residues at the interface that influence binding.
33068039	3	38	theme	virus	558:562	arg1	MGP1					580:583	MGP1	580:583	MGP1	580:583	Study of the experimental structure of Machupo virus glycoprotein 1 (MGP1) in complex with TfR and glycoprotein sequence homology has identified some residues at the interface that influence binding.
33068039	3	39	with	homology	632:639	arg1	TfR					602:604	TfR	602:604	TfR	602:604	Study of the experimental structure of Machupo virus glycoprotein 1 (MGP1) in complex with TfR and glycoprotein sequence homology has identified some residues at the interface that influence binding.
33068039	7	40	theme	N-glycan	1294:1301	arg1	modifications					1303:1315	posttranslational N-glycan modifications	1276:1315	posttranslational N-glycan modifications	1276:1315	We identified MGP1 binding hot spot residues, assessed the importance of posttranslational N-glycan modifications, and used a selection with random mutagenesis for affinity maturation.
33068039	2	41	theme	impenetrable	420:431	arg1	barrier					445:451	the impenetrable blood-brain barrier	416:451	the impenetrable blood-brain barrier that effectively blocks any such delivery into the brain	416:508	Similarly, the receptor interactions have been explored in biotechnological applications as a molecular system to ferry therapeutics across the cellular membranes and through the impenetrable blood-brain barrier that effectively blocks any such delivery into the brain.
33068039	5	42	theme	greater	910:916	arg1	understanding					918:930	a greater understanding	908:930	a greater understanding of MGP1 interactions with TfR	908:960	In pursuits for finding therapeutic solutions for the New World arenaviruses, and to gain a greater understanding of MGP1 interactions with TfR, it is crucial to understand the structure-sequence relationship driving the interface formation.
33068039	2	43	theme	molecular	335:343	arg1	system					345:350	a molecular system	333:350	a molecular system to ferry therapeutics across the cellular membranes and through the impenetrable blood-brain barrier that effectively blocks any such delivery into the brain	333:508	Similarly, the receptor interactions have been explored in biotechnological applications as a molecular system to ferry therapeutics across the cellular membranes and through the impenetrable blood-brain barrier that effectively blocks any such delivery into the brain.
33068039	2	43	theme	molecular	335:343	arg1	interactions					265:276	the receptor interactions	252:276	the receptor interactions	252:276	Similarly, the receptor interactions have been explored in biotechnological applications as a molecular system to ferry therapeutics across the cellular membranes and through the impenetrable blood-brain barrier that effectively blocks any such delivery into the brain.
33068039	1	44	theme	envelope	186:193	arg1	glycoprotein					195:206	its envelope glycoprotein	182:206	its envelope glycoprotein	182:206	Machupo virus, known to cause hemorrhagic fevers, enters human cells via binding with its envelope glycoprotein to transferrin receptor 1 (TfR).
33068039	0	45	theme	Machupo	37:43	arg1	glycoprotein					51:62	Machupo virus glycoprotein	37:62	Machupo virus glycoprotein	37:62	Tuning the binding interface between Machupo virus glycoprotein and human transferrin receptor.
33068039	9	46	used	used	1655:1658	arg2	variants					1639:1646	The engineered variants	1624:1646	The engineered variants	1624:1646	The engineered variants can be used to probe cellular internalization or the blood-brain barrier crossing to achieve greater understanding of TfR mediated internalization.
33068039	7	47	theme	spot	1234:1237	arg1	residues					1239:1246	MGP1 binding hot spot residues	1217:1246	MGP1 binding hot spot residues	1217:1246	We identified MGP1 binding hot spot residues, assessed the importance of posttranslational N-glycan modifications, and used a selection with random mutagenesis for affinity maturation.
33068039	4	48	theme	binding	778:784	arg1	potential					786:794	the binding potential	774:794	the binding potential between MGP1 and TfR	774:815	There are, however, no studies that have attempted to optimize the binding potential between MGP1 and TfR.
33068039	6	49	theme	solubilized	1172:1182	arg1	TfR					1198:1200	TfR	1198:1200	TfR	1198:1200	By displaying MGP1 on yeast surface we have examined the contributions of individual residues to the binding of solubilized ectodomain of TfR.
33068039	6	49	theme	solubilized	1172:1182	arg1	ectodomain					1184:1193	solubilized ectodomain	1172:1193	solubilized ectodomain of TfR	1172:1200	By displaying MGP1 on yeast surface we have examined the contributions of individual residues to the binding of solubilized ectodomain of TfR.
33068039	0	50	theme	binding	11:17	arg1	interface					19:27	the binding interface	7:27	the binding interface between Machupo virus glycoprotein and human transferrin receptor	7:93	Tuning the binding interface between Machupo virus glycoprotein and human transferrin receptor.
33068039	8	51	theme	substitutions	1609:1621	arg1	addition					1578:1585	the addition	1574:1585	the addition of further amino acid substitutions	1574:1621	We show that the optimized MGP1 variants can bind more strongly to TfR than the native MGP1, and there is an MGP1 sequence that retains binding in the absence of glycosylation, but with the addition of further amino acid substitutions.
33068039	3	52	theme	sequence	623:630	arg1	homology					632:639	glycoprotein sequence homology	610:639	glycoprotein sequence homology	610:639	Study of the experimental structure of Machupo virus glycoprotein 1 (MGP1) in complex with TfR and glycoprotein sequence homology has identified some residues at the interface that influence binding.
33068039	5	53	from	pursuits	821:828	arg1	crucial					969:975	crucial	969:975	crucial	969:975	In pursuits for finding therapeutic solutions for the New World arenaviruses, and to gain a greater understanding of MGP1 interactions with TfR, it is crucial to understand the structure-sequence relationship driving the interface formation.
33068039	7	54	theme	posttranslational	1276:1292	arg1	modifications					1303:1315	posttranslational N-glycan modifications	1276:1315	posttranslational N-glycan modifications	1276:1315	We identified MGP1 binding hot spot residues, assessed the importance of posttranslational N-glycan modifications, and used a selection with random mutagenesis for affinity maturation.
33068039	1	55	with	binding	169:175	arg1	glycoprotein					195:206	its envelope glycoprotein	182:206	its envelope glycoprotein	182:206	Machupo virus, known to cause hemorrhagic fevers, enters human cells via binding with its envelope glycoprotein to transferrin receptor 1 (TfR).
33068039	5	56	theme	interactions	940:951	arg1	understanding					918:930	a greater understanding	908:930	a greater understanding of MGP1 interactions with TfR	908:960	In pursuits for finding therapeutic solutions for the New World arenaviruses, and to gain a greater understanding of MGP1 interactions with TfR, it is crucial to understand the structure-sequence relationship driving the interface formation.
33068039	0	57	theme	virus	45:49	arg1	glycoprotein					51:62	Machupo virus glycoprotein	37:62	Machupo virus glycoprotein	37:62	Tuning the binding interface between Machupo virus glycoprotein and human transferrin receptor.
33068039	8	58	theme	glycosylation	1550:1562	arg1	absence					1539:1545	the absence	1535:1545	the absence of glycosylation	1535:1562	We show that the optimized MGP1 variants can bind more strongly to TfR than the native MGP1, and there is an MGP1 sequence that retains binding in the absence of glycosylation, but with the addition of further amino acid substitutions.
33068039	5	59	theme	structure-sequence	995:1012	arg1	relationship					1014:1025	the structure-sequence relationship	991:1025	the structure-sequence relationship driving the interface formation	991:1057	In pursuits for finding therapeutic solutions for the New World arenaviruses, and to gain a greater understanding of MGP1 interactions with TfR, it is crucial to understand the structure-sequence relationship driving the interface formation.
33068039	8	60	theme	MGP1	1415:1418	arg1	variants					1420:1427	the optimized MGP1 variants	1401:1427	the optimized MGP1 variants	1401:1427	We show that the optimized MGP1 variants can bind more strongly to TfR than the native MGP1, and there is an MGP1 sequence that retains binding in the absence of glycosylation, but with the addition of further amino acid substitutions.
33068039	3	61	gly	glycoprotein	610:621	arg1	glycoprotein					610:621	glycoprotein sequence homology	610:639	glycoprotein sequence homology	610:639	Study of the experimental structure of Machupo virus glycoprotein 1 (MGP1) in complex with TfR and glycoprotein sequence homology has identified some residues at the interface that influence binding.
33068039	9	62	theme	barrier	1713:1719	arg1	crossing					1721:1728	the blood-brain barrier crossing	1697:1728	the blood-brain barrier crossing	1697:1728	The engineered variants can be used to probe cellular internalization or the blood-brain barrier crossing to achieve greater understanding of TfR mediated internalization.
33068039	7	63	theme	random	1344:1349	arg1	mutagenesis					1351:1361	random mutagenesis	1344:1361	random mutagenesis	1344:1361	We identified MGP1 binding hot spot residues, assessed the importance of posttranslational N-glycan modifications, and used a selection with random mutagenesis for affinity maturation.
33068039	6	64	theme	TfR	1198:1200	arg1	TfR					1198:1200	TfR	1198:1200	TfR	1198:1200	By displaying MGP1 on yeast surface we have examined the contributions of individual residues to the binding of solubilized ectodomain of TfR.
33068039	6	64	theme	TfR	1198:1200	arg1	ectodomain					1184:1193	solubilized ectodomain	1172:1193	solubilized ectodomain of TfR	1172:1200	By displaying MGP1 on yeast surface we have examined the contributions of individual residues to the binding of solubilized ectodomain of TfR.
33068039	3	65	gly	glycoprotein	564:575	arg1	glycoprotein					564:575	Machupo virus glycoprotein 1	550:577	Machupo virus glycoprotein 1 (MGP1)	550:584	Study of the experimental structure of Machupo virus glycoprotein 1 (MGP1) in complex with TfR and glycoprotein sequence homology has identified some residues at the interface that influence binding.
33068039	3	65	gly	glycoprotein	564:575	arg1	MGP1					580:583	MGP1	580:583	MGP1	580:583	Study of the experimental structure of Machupo virus glycoprotein 1 (MGP1) in complex with TfR and glycoprotein sequence homology has identified some residues at the interface that influence binding.
33068039	8	66	theme	acid	1604:1607	arg1	substitutions					1609:1621	further amino acid substitutions	1590:1621	further amino acid substitutions	1590:1621	We show that the optimized MGP1 variants can bind more strongly to TfR than the native MGP1, and there is an MGP1 sequence that retains binding in the absence of glycosylation, but with the addition of further amino acid substitutions.
33068039	1	67	theme	transferrin	211:221	arg1	receptor					223:230	transferrin receptor 1	211:232	transferrin receptor 1 (TfR)	211:238	Machupo virus, known to cause hemorrhagic fevers, enters human cells via binding with its envelope glycoprotein to transferrin receptor 1 (TfR).
33068039	1	67	theme	transferrin	211:221	arg1	TfR					235:237	TfR	235:237	TfR	235:237	Machupo virus, known to cause hemorrhagic fevers, enters human cells via binding with its envelope glycoprotein to transferrin receptor 1 (TfR).
33068039	7	68	used	used	1322:1325	arg2	We					1203:1204	We	1203:1204	We	1203:1204	We identified MGP1 binding hot spot residues, assessed the importance of posttranslational N-glycan modifications, and used a selection with random mutagenesis for affinity maturation.
33068039	8	69	theme	native	1468:1473	arg1	MGP1					1475:1478	the native MGP1	1464:1478	the native MGP1	1464:1478	We show that the optimized MGP1 variants can bind more strongly to TfR than the native MGP1, and there is an MGP1 sequence that retains binding in the absence of glycosylation, but with the addition of further amino acid substitutions.
33068039	3	70	theme	glycoprotein	610:621	arg1	homology					632:639	glycoprotein sequence homology	610:639	glycoprotein sequence homology	610:639	Study of the experimental structure of Machupo virus glycoprotein 1 (MGP1) in complex with TfR and glycoprotein sequence homology has identified some residues at the interface that influence binding.
33068039	1	71	theme	hemorrhagic	126:136	arg1	fevers					138:143	hemorrhagic fevers	126:143	hemorrhagic fevers	126:143	Machupo virus, known to cause hemorrhagic fevers, enters human cells via binding with its envelope glycoprotein to transferrin receptor 1 (TfR).
33068039	0	72	theme	human	68:72	arg1	receptor					86:93	human transferrin receptor	68:93	human transferrin receptor	68:93	Tuning the binding interface between Machupo virus glycoprotein and human transferrin receptor.
33068039	5	73	theme	interface	1039:1047	arg1	formation					1049:1057	the interface formation	1035:1057	the interface formation	1035:1057	In pursuits for finding therapeutic solutions for the New World arenaviruses, and to gain a greater understanding of MGP1 interactions with TfR, it is crucial to understand the structure-sequence relationship driving the interface formation.
33068039	6	74	theme	ectodomain	1184:1193	arg1	binding					1161:1167	the binding	1157:1167	the binding of solubilized ectodomain of TfR	1157:1200	By displaying MGP1 on yeast surface we have examined the contributions of individual residues to the binding of solubilized ectodomain of TfR.
33068039	2	75	theme	ferry	355:359	arg1	therapeutics					361:372	ferry therapeutics	355:372	ferry therapeutics	355:372	Similarly, the receptor interactions have been explored in biotechnological applications as a molecular system to ferry therapeutics across the cellular membranes and through the impenetrable blood-brain barrier that effectively blocks any such delivery into the brain.
33068039	7	76	theme	MGP1	1217:1220	arg1	spot					1234:1237	MGP1 binding hot spot	1217:1237	MGP1 binding hot spot residues	1217:1246	We identified MGP1 binding hot spot residues, assessed the importance of posttranslational N-glycan modifications, and used a selection with random mutagenesis for affinity maturation.
33068039	0	77	gly	glycoprotein	51:62	arg1	glycoprotein					51:62	Machupo virus glycoprotein	37:62	Machupo virus glycoprotein	37:62	Tuning the binding interface between Machupo virus glycoprotein and human transferrin receptor.
33068039	5	78	theme	New	872:874	arg1	arenaviruses					882:893	the New World arenaviruses	868:893	the New World arenaviruses	868:893	In pursuits for finding therapeutic solutions for the New World arenaviruses, and to gain a greater understanding of MGP1 interactions with TfR, it is crucial to understand the structure-sequence relationship driving the interface formation.
33068039	7	79	theme	binding	1222:1228	arg1	spot					1234:1237	MGP1 binding hot spot	1217:1237	MGP1 binding hot spot residues	1217:1246	We identified MGP1 binding hot spot residues, assessed the importance of posttranslational N-glycan modifications, and used a selection with random mutagenesis for affinity maturation.
33514729	1	0	from	diets	178:182	arg1	shift					134:138	the shift	130:138	the shift from nitrogen-rich carnivore/omnivore diets to nitrogen-poor herbivorous diets	130:217	Across the evolutionary history of insects, the shift from nitrogen-rich carnivore/omnivore diets to nitrogen-poor herbivorous diets was made possible through symbiosis with microbes.
33514729	0	1	theme	turtle	73:78	arg1	ants					80:83	herbivorous turtle ants	61:83	herbivorous turtle ants	61:83	Gut bacteria are essential for normal cuticle development in herbivorous turtle ants.
33514729	4	2	from	cuticle	878:884	arg1	formation					808:816	the formation	804:816	the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle	804:884	To gain insights into nitrogen assimilation in the ant cuticle we use gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy to demonstrate that gut bacteria contribute to the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle.
33514729	1	3	theme	nitrogen-rich	145:157	arg1	diets					178:182	nitrogen-rich carnivore/omnivore diets	145:182	nitrogen-rich carnivore/omnivore diets	145:182	Across the evolutionary history of insects, the shift from nitrogen-rich carnivore/omnivore diets to nitrogen-poor herbivorous diets was made possible through symbiosis with microbes.
33514729	4	4	theme	nitrogen	575:582	arg1	assimilation					584:595	nitrogen assimilation	575:595	nitrogen assimilation in the ant cuticle	575:614	To gain insights into nitrogen assimilation in the ant cuticle we use gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy to demonstrate that gut bacteria contribute to the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle.
33514729	4	5	theme	gut	777:779	arg1	bacteria					781:788	gut bacteria	777:788	gut bacteria	777:788	To gain insights into nitrogen assimilation in the ant cuticle we use gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy to demonstrate that gut bacteria contribute to the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle.
33514729	4	6	theme	isotopic	655:662	arg1	enrichment					664:673	15N isotopic enrichment	651:673	15N isotopic enrichment	651:673	To gain insights into nitrogen assimilation in the ant cuticle we use gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy to demonstrate that gut bacteria contribute to the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle.
33514729	5	7	from	nutrients	1101:1109	arg1	flow					1091:1094	the nitrogen flow	1078:1094	the nitrogen flow from nutrients through bacteria	1078:1126	This study identifies the cuticular components which are nitrogen-enriched by gut bacteria, highlighting the role of symbionts in insect evolution, and provides a framework for understanding the nitrogen flow from nutrients through bacteria into the insect cuticle.
33514729	0	8	from	development	46:56	arg1	ants					80:83	herbivorous turtle ants	61:83	herbivorous turtle ants	61:83	Gut bacteria are essential for normal cuticle development in herbivorous turtle ants.
33514729	4	9	from	formation	808:816	arg1	cuticle					878:884	the cuticle	874:884	the cuticle	874:884	To gain insights into nitrogen assimilation in the ant cuticle we use gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy to demonstrate that gut bacteria contribute to the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle.
33514729	4	10	theme	magnetic	725:732	arg1	resonance					734:742	gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance	623:742	gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy	623:755	To gain insights into nitrogen assimilation in the ant cuticle we use gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy to demonstrate that gut bacteria contribute to the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle.
33514729	4	11	theme	spectrometry	695:706	arg1	resonance					734:742	gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance	623:742	gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy	623:755	To gain insights into nitrogen assimilation in the ant cuticle we use gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy to demonstrate that gut bacteria contribute to the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle.
33514729	4	12	theme	15N	713:715	arg1	resonance					734:742	gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance	623:742	gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy	623:755	To gain insights into nitrogen assimilation in the ant cuticle we use gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy to demonstrate that gut bacteria contribute to the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle.
33514729	1	13	theme	carnivore/omnivore	159:176	arg1	diets					178:182	nitrogen-rich carnivore/omnivore diets	145:182	nitrogen-rich carnivore/omnivore diets	145:182	Across the evolutionary history of insects, the shift from nitrogen-rich carnivore/omnivore diets to nitrogen-poor herbivorous diets was made possible through symbiosis with microbes.
33514729	5	14	theme	cuticular	913:921	arg1	components					923:932	the cuticular components	909:932	the cuticular components which are nitrogen-enriched by gut bacteria, highlighting the role of symbionts in insect evolution	909:1032	This study identifies the cuticular components which are nitrogen-enriched by gut bacteria, highlighting the role of symbionts in insect evolution, and provides a framework for understanding the nitrogen flow from nutrients through bacteria into the insect cuticle.
33514729	5	14	theme	cuticular	913:921	arg1	nitrogen-enriched					944:960	nitrogen-enriched	944:960	nitrogen-enriched	944:960	This study identifies the cuticular components which are nitrogen-enriched by gut bacteria, highlighting the role of symbionts in insect evolution, and provides a framework for understanding the nitrogen flow from nutrients through bacteria into the insect cuticle.
33514729	2	15	theme	turtle	286:291	arg1	Cephalotes					298:307	Cephalotes	298:307	Cephalotes	298:307	The herbivorous turtle ants Cephalotes possess a conserved gut microbiome which enriches the nutrient composition by recycling nitrogen-rich metabolic waste to increase the production of amino acids.
33514729	2	15	theme	turtle	286:291	arg1	ants					293:296	The herbivorous turtle ants	270:296	The herbivorous turtle ants Cephalotes	270:307	The herbivorous turtle ants Cephalotes possess a conserved gut microbiome which enriches the nutrient composition by recycling nitrogen-rich metabolic waste to increase the production of amino acids.
33514729	4	16	theme	15N	651:653	arg1	enrichment					664:673	15N isotopic enrichment	651:673	15N isotopic enrichment	651:673	To gain insights into nitrogen assimilation in the ant cuticle we use gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy to demonstrate that gut bacteria contribute to the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle.
33514729	4	17	from	chitin	864:869	arg1	cuticle					878:884	the cuticle	874:884	the cuticle	874:884	To gain insights into nitrogen assimilation in the ant cuticle we use gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy to demonstrate that gut bacteria contribute to the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle.
33514729	0	18	theme	Gut	0:2	arg1	bacteria					4:11	Gut bacteria	0:11	Gut bacteria	0:11	Gut bacteria are essential for normal cuticle development in herbivorous turtle ants.
33514729	4	19	theme	chitin	864:869	arg1	formation					808:816	the formation	804:816	the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle	804:884	To gain insights into nitrogen assimilation in the ant cuticle we use gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy to demonstrate that gut bacteria contribute to the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle.
33514729	2	20	theme	gut	329:331	arg1	microbiome					333:342	a conserved gut microbiome	317:342	a conserved gut microbiome which enriches the nutrient composition by recycling nitrogen-rich metabolic waste to increase the production of amino acids	317:467	The herbivorous turtle ants Cephalotes possess a conserved gut microbiome which enriches the nutrient composition by recycling nitrogen-rich metabolic waste to increase the production of amino acids.
33514729	1	21	theme	nitrogen-poor	187:199	arg1	diets					213:217	nitrogen-poor herbivorous diets	187:217	nitrogen-poor herbivorous diets	187:217	Across the evolutionary history of insects, the shift from nitrogen-rich carnivore/omnivore diets to nitrogen-poor herbivorous diets was made possible through symbiosis with microbes.
33514729	4	22	theme	ant	604:606	arg1	cuticle					608:614	the ant cuticle	600:614	the ant cuticle	600:614	To gain insights into nitrogen assimilation in the ant cuticle we use gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy to demonstrate that gut bacteria contribute to the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle.
33514729	4	23	theme	resonance	734:742	arg1	spectroscopy					744:755	gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy	623:755	gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy	623:755	To gain insights into nitrogen assimilation in the ant cuticle we use gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy to demonstrate that gut bacteria contribute to the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle.
33514729	2	24	theme	conserved	319:327	arg1	microbiome					333:342	a conserved gut microbiome	317:342	a conserved gut microbiome which enriches the nutrient composition by recycling nitrogen-rich metabolic waste to increase the production of amino acids	317:467	The herbivorous turtle ants Cephalotes possess a conserved gut microbiome which enriches the nutrient composition by recycling nitrogen-rich metabolic waste to increase the production of amino acids.
33514729	2	25	theme	amino	457:461	arg1	acids					463:467	amino acids	457:467	amino acids	457:467	The herbivorous turtle ants Cephalotes possess a conserved gut microbiome which enriches the nutrient composition by recycling nitrogen-rich metabolic waste to increase the production of amino acids.
33514729	2	26	theme	acids	463:467	arg1	production					443:452	the production	439:452	the production of amino acids	439:467	The herbivorous turtle ants Cephalotes possess a conserved gut microbiome which enriches the nutrient composition by recycling nitrogen-rich metabolic waste to increase the production of amino acids.
33514729	5	27	theme	gut	965:967	arg1	bacteria					969:976	gut bacteria	965:976	gut bacteria	965:976	This study identifies the cuticular components which are nitrogen-enriched by gut bacteria, highlighting the role of symbionts in insect evolution, and provides a framework for understanding the nitrogen flow from nutrients through bacteria into the insect cuticle.
33514729	5	28	theme	nitrogen	1082:1089	arg1	flow					1091:1094	the nitrogen flow	1078:1094	the nitrogen flow from nutrients through bacteria	1078:1126	This study identifies the cuticular components which are nitrogen-enriched by gut bacteria, highlighting the role of symbionts in insect evolution, and provides a framework for understanding the nitrogen flow from nutrients through bacteria into the insect cuticle.
33514729	1	29	theme	evolutionary	97:108	arg1	history					110:116	the evolutionary history	93:116	the evolutionary history of insects	93:127	Across the evolutionary history of insects, the shift from nitrogen-rich carnivore/omnivore diets to nitrogen-poor herbivorous diets was made possible through symbiosis with microbes.
33514729	4	30	theme	gut	623:625	arg1	manipulation					637:648	gut bacterial manipulation	623:648	gut bacterial manipulation	623:648	To gain insights into nitrogen assimilation in the ant cuticle we use gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy to demonstrate that gut bacteria contribute to the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle.
33514729	0	31	theme	normal	31:36	arg1	development					46:56	normal cuticle development	31:56	normal cuticle development in herbivorous turtle ants	31:83	Gut bacteria are essential for normal cuticle development in herbivorous turtle ants.
33514729	2	32	contain	possess	309:315	arg2	microbiome					333:342	a conserved gut microbiome	317:342	a conserved gut microbiome which enriches the nutrient composition by recycling nitrogen-rich metabolic waste to increase the production of amino acids	317:467	The herbivorous turtle ants Cephalotes possess a conserved gut microbiome which enriches the nutrient composition by recycling nitrogen-rich metabolic waste to increase the production of amino acids.
33514729	2	32	contain	possess	309:315	arg1	Cephalotes					298:307	Cephalotes	298:307	Cephalotes	298:307	The herbivorous turtle ants Cephalotes possess a conserved gut microbiome which enriches the nutrient composition by recycling nitrogen-rich metabolic waste to increase the production of amino acids.
33514729	2	32	contain	possess	309:315	arg1	ants					293:296	The herbivorous turtle ants	270:296	The herbivorous turtle ants Cephalotes	270:307	The herbivorous turtle ants Cephalotes possess a conserved gut microbiome which enriches the nutrient composition by recycling nitrogen-rich metabolic waste to increase the production of amino acids.
33514729	2	33	theme	metabolic	411:419	arg1	waste					421:425	nitrogen-rich metabolic waste	397:425	nitrogen-rich metabolic waste	397:425	The herbivorous turtle ants Cephalotes possess a conserved gut microbiome which enriches the nutrient composition by recycling nitrogen-rich metabolic waste to increase the production of amino acids.
33514729	4	34	theme	mass	690:693	arg1	spectrometry					695:706	isotope-ratio mass spectrometry	676:706	isotope-ratio mass spectrometry	676:706	To gain insights into nitrogen assimilation in the ant cuticle we use gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy to demonstrate that gut bacteria contribute to the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle.
33514729	4	35	theme	proteins	821:828	arg1	formation					808:816	the formation	804:816	the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle	804:884	To gain insights into nitrogen assimilation in the ant cuticle we use gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy to demonstrate that gut bacteria contribute to the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle.
33514729	4	36	from	cross-linkers	845:857	arg1	cuticle					878:884	the cuticle	874:884	the cuticle	874:884	To gain insights into nitrogen assimilation in the ant cuticle we use gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy to demonstrate that gut bacteria contribute to the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle.
33514729	4	37	theme	manipulation	637:648	arg1	resonance					734:742	gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance	623:742	gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy	623:755	To gain insights into nitrogen assimilation in the ant cuticle we use gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy to demonstrate that gut bacteria contribute to the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle.
33514729	4	38	theme	cross-linkers	845:857	arg1	formation					808:816	the formation	804:816	the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle	804:884	To gain insights into nitrogen assimilation in the ant cuticle we use gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy to demonstrate that gut bacteria contribute to the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle.
33514729	2	39	theme	nutrient	363:370	arg1	composition					372:382	the nutrient composition	359:382	the nutrient composition	359:382	The herbivorous turtle ants Cephalotes possess a conserved gut microbiome which enriches the nutrient composition by recycling nitrogen-rich metabolic waste to increase the production of amino acids.
33514729	0	40	theme	cuticle	38:44	arg1	development					46:56	normal cuticle development	31:56	normal cuticle development in herbivorous turtle ants	31:83	Gut bacteria are essential for normal cuticle development in herbivorous turtle ants.
33514729	4	41	dep	use	619:621	arg1	gain					556:559	gain	556:559	To gain insights into nitrogen assimilation in the ant cuticle	553:614	To gain insights into nitrogen assimilation in the ant cuticle we use gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy to demonstrate that gut bacteria contribute to the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle.
33514729	4	42	theme	catecholamine	831:843	arg1	cross-linkers					845:857	catecholamine cross-linkers	831:857	catecholamine cross-linkers	831:857	To gain insights into nitrogen assimilation in the ant cuticle we use gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy to demonstrate that gut bacteria contribute to the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle.
33514729	4	43	theme	enrichment	664:673	arg1	resonance					734:742	gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance	623:742	gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy	623:755	To gain insights into nitrogen assimilation in the ant cuticle we use gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy to demonstrate that gut bacteria contribute to the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle.
33514729	5	44	theme	symbionts	1004:1012	arg1	role					996:999	the role	992:999	the role of symbionts in insect evolution	992:1032	This study identifies the cuticular components which are nitrogen-enriched by gut bacteria, highlighting the role of symbionts in insect evolution, and provides a framework for understanding the nitrogen flow from nutrients through bacteria into the insect cuticle.
33514729	2	45	theme	herbivorous	274:284	arg1	Cephalotes					298:307	Cephalotes	298:307	Cephalotes	298:307	The herbivorous turtle ants Cephalotes possess a conserved gut microbiome which enriches the nutrient composition by recycling nitrogen-rich metabolic waste to increase the production of amino acids.
33514729	2	45	theme	herbivorous	274:284	arg1	ants					293:296	The herbivorous turtle ants	270:296	The herbivorous turtle ants Cephalotes	270:307	The herbivorous turtle ants Cephalotes possess a conserved gut microbiome which enriches the nutrient composition by recycling nitrogen-rich metabolic waste to increase the production of amino acids.
33514729	4	46	from	proteins	821:828	arg1	cuticle					878:884	the cuticle	874:884	the cuticle	874:884	To gain insights into nitrogen assimilation in the ant cuticle we use gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy to demonstrate that gut bacteria contribute to the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle.
33514729	1	47	theme	insects	121:127	arg1	history					110:116	the evolutionary history	93:116	the evolutionary history of insects	93:127	Across the evolutionary history of insects, the shift from nitrogen-rich carnivore/omnivore diets to nitrogen-poor herbivorous diets was made possible through symbiosis with microbes.
33514729	4	48	from	assimilation	584:595	arg1	cuticle					608:614	the ant cuticle	600:614	the ant cuticle	600:614	To gain insights into nitrogen assimilation in the ant cuticle we use gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy to demonstrate that gut bacteria contribute to the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle.
33514729	1	49	with	symbiosis	245:253	arg1	microbes					260:267	microbes	260:267	microbes	260:267	Across the evolutionary history of insects, the shift from nitrogen-rich carnivore/omnivore diets to nitrogen-poor herbivorous diets was made possible through symbiosis with microbes.
33514729	4	50	theme	nuclear	717:723	arg1	resonance					734:742	gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance	623:742	gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy	623:755	To gain insights into nitrogen assimilation in the ant cuticle we use gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy to demonstrate that gut bacteria contribute to the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle.
33514729	3	51	dep	extent	545:550	arg1	what					540:543	what	540:543	what	540:543	This enrichment is assumed to benefit the host, but we do not know to what extent.
33514729	0	52	theme	herbivorous	61:71	arg1	ants					80:83	herbivorous turtle ants	61:83	herbivorous turtle ants	61:83	Gut bacteria are essential for normal cuticle development in herbivorous turtle ants.
33514729	5	53	theme	insect	1017:1022	arg1	evolution					1024:1032	insect evolution	1017:1032	insect evolution	1017:1032	This study identifies the cuticular components which are nitrogen-enriched by gut bacteria, highlighting the role of symbionts in insect evolution, and provides a framework for understanding the nitrogen flow from nutrients through bacteria into the insect cuticle.
33514729	1	54	theme	herbivorous	201:211	arg1	diets					213:217	nitrogen-poor herbivorous diets	187:217	nitrogen-poor herbivorous diets	187:217	Across the evolutionary history of insects, the shift from nitrogen-rich carnivore/omnivore diets to nitrogen-poor herbivorous diets was made possible through symbiosis with microbes.
33514729	2	55	theme	nitrogen-rich	397:409	arg1	waste					421:425	nitrogen-rich metabolic waste	397:425	nitrogen-rich metabolic waste	397:425	The herbivorous turtle ants Cephalotes possess a conserved gut microbiome which enriches the nutrient composition by recycling nitrogen-rich metabolic waste to increase the production of amino acids.
33514729	5	56	theme	insect	1137:1142	arg1	cuticle					1144:1150	the insect cuticle	1133:1150	the insect cuticle	1133:1150	This study identifies the cuticular components which are nitrogen-enriched by gut bacteria, highlighting the role of symbionts in insect evolution, and provides a framework for understanding the nitrogen flow from nutrients through bacteria into the insect cuticle.
33514729	4	57	theme	bacterial	627:635	arg1	manipulation					637:648	gut bacterial manipulation	623:648	gut bacterial manipulation	623:648	To gain insights into nitrogen assimilation in the ant cuticle we use gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy to demonstrate that gut bacteria contribute to the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle.
33514729	4	58	theme	isotope-ratio	676:688	arg1	spectrometry					695:706	isotope-ratio mass spectrometry	676:706	isotope-ratio mass spectrometry	676:706	To gain insights into nitrogen assimilation in the ant cuticle we use gut bacterial manipulation, 15N isotopic enrichment, isotope-ratio mass spectrometry, and 15N nuclear magnetic resonance spectroscopy to demonstrate that gut bacteria contribute to the formation of proteins, catecholamine cross-linkers, and chitin in the cuticle.
33514729	5	59	from	role	996:999	arg1	evolution					1024:1032	insect evolution	1017:1032	insect evolution	1017:1032	This study identifies the cuticular components which are nitrogen-enriched by gut bacteria, highlighting the role of symbionts in insect evolution, and provides a framework for understanding the nitrogen flow from nutrients through bacteria into the insect cuticle.
32099019	12	0	dep	coli	1997:2000	arg1	gram-negative					2003:2015	gram-negative	2003:2015	gram-negative	2003:2015	The drug delivery property was further justified through an antibacterial analysis against Escherichia coli (gram-negative) and Bacillus subtilis (gram-positive).
32099019	11	1	theme	aluminosilicate-conjugated	1789:1814	arg1	ampicillin					1816:1825	the aptasensing aluminosilicate-conjugated ampicillin	1773:1825	the aptasensing aluminosilicate-conjugated ampicillin	1773:1825	The above was justified by an enzyme-linked apta-sorbent assay, which claimed that the limit of the aptasensing aluminosilicate-conjugated ampicillin was two-fold higher than that in the absence of the nanocomposite.
32099019	7	2	theme	X-ray	1177:1181	arg1	patterns					1195:1202	X-ray diffraction patterns	1177:1202	X-ray diffraction patterns	1177:1202	X-ray diffraction patterns showed a synthesis of faceted and cubic aluminosilicate crystals in the nanocomposites.
32099019	12	3	theme	delivery	1903:1910	arg1	property					1912:1919	The drug delivery property	1894:1919	The drug delivery property	1894:1919	The drug delivery property was further justified through an antibacterial analysis against Escherichia coli (gram-negative) and Bacillus subtilis (gram-positive).
32099019	7	4	theme	aluminosilicate	1244:1258	arg1	crystals					1260:1267	faceted and cubic aluminosilicate crystals	1226:1267	faceted and cubic aluminosilicate crystals	1226:1267	X-ray diffraction patterns showed a synthesis of faceted and cubic aluminosilicate crystals in the nanocomposites.
32099019	9	5	theme	thermogravimetric	1535:1551	arg1	analysis					1553:1560	a thermogravimetric analysis	1533:1560	a thermogravimetric analysis	1533:1560	The thermal capacity of the nanocomposite was examined by a thermogravimetric analysis.
32099019	3	6	theme	structural	582:591	arg1	characteristics					612:626	its structural and physiochemical characteristics	578:626	its structural and physiochemical characteristics	578:626	The synthesized aluminosilicate nanocomposite was characterized to justify its structural and physiochemical characteristics.
32099019	8	7	dep	Fourier	1434:1440	arg1	transform					1442:1450	transform	1442:1450	transform infrared spectroscopy	1442:1472	The presence of silica and aluminum was further proven by X-ray photoelectron spectroscopy, and the functional groups were recognized through Fourier transform infrared spectroscopy.
32099019	4	8	theme	transmission	688:699	arg1	microscopy					710:719	field-emission transmission electron microscopy	673:719	field-emission transmission electron microscopy	673:719	A morphological analysis was performed with field-emission transmission electron microscopy, and scanning electron microscopy revealed the size of the aluminosilicate nanocomposite to be ~25 nm, while also confirming a uniformly spherical-shaped nanostructure.
32099019	0	9	theme	Incinerated	36:46	arg1	Ash					70:72	Incinerated Chinese Holy Joss Fly Ash	36:72	Incinerated Chinese Holy Joss Fly Ash	36:72	Aluminosilicate Nanocomposites from Incinerated Chinese Holy Joss Fly Ash: A Potential Nanocarrier for Drug Cargos.
32099019	1	10	theme	holy	193:196	arg1	paper					203:207	Chinese holy joss paper	185:207	Chinese holy joss paper	185:207	An incredible amount of joss fly ash is produced from the burning of Chinese holy joss paper; thus, an excellent method of recycling joss fly ash waste to extract aluminosilicate nanocomposites is explored.
32099019	0	11	from	Ash	70:72	arg1	Nanocomposites					16:29	Aluminosilicate Nanocomposites	0:29	Aluminosilicate Nanocomposites from Incinerated Chinese Holy Joss Fly Ash: A Potential Nanocarrier for Drug Cargos.	0:114	Aluminosilicate Nanocomposites from Incinerated Chinese Holy Joss Fly Ash: A Potential Nanocarrier for Drug Cargos.
32099019	3	12	theme	physiochemical	597:610	arg1	characteristics					612:626	its structural and physiochemical characteristics	578:626	its structural and physiochemical characteristics	578:626	The synthesized aluminosilicate nanocomposite was characterized to justify its structural and physiochemical characteristics.
32099019	11	13	theme	nanocomposite	1879:1891	arg1	absence					1864:1870	the absence	1860:1870	the absence of the nanocomposite	1860:1891	The above was justified by an enzyme-linked apta-sorbent assay, which claimed that the limit of the aptasensing aluminosilicate-conjugated ampicillin was two-fold higher than that in the absence of the nanocomposite.
32099019	6	14	theme	roughness	1076:1084	arg1	distribution					1099:1110	The roughness and particle distribution	1072:1110	The roughness and particle distribution	1072:1110	The roughness and particle distribution were analyzed using atomic force microscopy and a zeta analysis.
32099019	2	15	theme	alkaline	482:489	arg1	treatments					491:500	straightforward experimental procedure involving acidic and alkaline treatments	422:500	treatments	491:500	The present research aims to introduce a novel method to recycle joss fly ash through a simple and straightforward experimental procedure involving acidic and alkaline treatments.
32099019	8	16	theme	functional	1392:1401	arg1	groups					1403:1408	the functional groups	1388:1408	the functional groups	1388:1408	The presence of silica and aluminum was further proven by X-ray photoelectron spectroscopy, and the functional groups were recognized through Fourier transform infrared spectroscopy.
32099019	1	17	theme	aluminosilicate	279:293	arg1	nanocomposites					295:308	extract aluminosilicate nanocomposites	271:308	extract aluminosilicate nanocomposites	271:308	An incredible amount of joss fly ash is produced from the burning of Chinese holy joss paper; thus, an excellent method of recycling joss fly ash waste to extract aluminosilicate nanocomposites is explored.
32099019	1	18	theme	joss	140:143	arg1	ash					149:151	joss fly ash	140:151	joss fly ash	140:151	An incredible amount of joss fly ash is produced from the burning of Chinese holy joss paper; thus, an excellent method of recycling joss fly ash waste to extract aluminosilicate nanocomposites is explored.
32099019	1	19	theme	ash	149:151	arg1	amount					130:135	An incredible amount	116:135	An incredible amount of joss fly ash	116:151	An incredible amount of joss fly ash is produced from the burning of Chinese holy joss paper; thus, an excellent method of recycling joss fly ash waste to extract aluminosilicate nanocomposites is explored.
32099019	1	19	theme	ash	149:151	arg1	ash					149:151	joss fly ash	140:151	joss fly ash	140:151	An incredible amount of joss fly ash is produced from the burning of Chinese holy joss paper; thus, an excellent method of recycling joss fly ash waste to extract aluminosilicate nanocomposites is explored.
32099019	0	20	theme	Drug	103:106	arg1	Cargos					108:113	Drug Cargos	103:113	Drug Cargos	103:113	Aluminosilicate Nanocomposites from Incinerated Chinese Holy Joss Fly Ash: A Potential Nanocarrier for Drug Cargos.
32099019	6	21	theme	force	1139:1143	arg1	microscopy					1145:1154	atomic force microscopy	1132:1154	atomic force microscopy	1132:1154	The roughness and particle distribution were analyzed using atomic force microscopy and a zeta analysis.
32099019	4	22	theme	nanocomposite	796:808	arg1	size					768:771	the size	764:771	the size of the aluminosilicate nanocomposite to be ~25 nm	764:821	A morphological analysis was performed with field-emission transmission electron microscopy, and scanning electron microscopy revealed the size of the aluminosilicate nanocomposite to be ~25 nm, while also confirming a uniformly spherical-shaped nanostructure.
32099019	0	23	theme	Aluminosilicate	0:14	arg1	Nanocomposites					16:29	Aluminosilicate Nanocomposites	0:29	Aluminosilicate Nanocomposites from Incinerated Chinese Holy Joss Fly Ash: A Potential Nanocarrier for Drug Cargos.	0:114	Aluminosilicate Nanocomposites from Incinerated Chinese Holy Joss Fly Ash: A Potential Nanocarrier for Drug Cargos.
32099019	2	24	theme	fly	393:395	arg1	ash					397:399	joss fly ash	388:399	joss fly ash	388:399	The present research aims to introduce a novel method to recycle joss fly ash through a simple and straightforward experimental procedure involving acidic and alkaline treatments.
32099019	10	25	theme	promising	1603:1611	arg1	application					1613:1623	the promising application	1599:1623	the promising application of aluminosilicate nanocomposites as drug carriers	1599:1674	In addition, the research suggested the promising application of aluminosilicate nanocomposites as drug carriers.
32099019	0	26	dep	Nanocomposites	16:29	arg1	Nanocarrier					87:97	A Potential Nanocarrier	75:97	Aluminosilicate Nanocomposites from Incinerated Chinese Holy Joss Fly Ash: A Potential Nanocarrier for Drug Cargos.	0:114	Aluminosilicate Nanocomposites from Incinerated Chinese Holy Joss Fly Ash: A Potential Nanocarrier for Drug Cargos.
32099019	5	27	theme	elemental	894:902	arg1	composition					904:914	The elemental composition	890:914	The elemental composition	890:914	The elemental composition was measured by energy dispersive spectroscopy and revealed the Si to Al ratio to be 13.24 to 7.96, showing the high purity of the extracted nanocomposite.
32099019	1	28	theme	joss	249:252	arg1	ash					258:260	recycling joss fly ash	239:260	recycling joss fly ash waste	239:266	An incredible amount of joss fly ash is produced from the burning of Chinese holy joss paper; thus, an excellent method of recycling joss fly ash waste to extract aluminosilicate nanocomposites is explored.
32099019	5	29	dep	7.96	1010:1013	arg1	to					1007:1008	to	1007:1008	to	1007:1008	The elemental composition was measured by energy dispersive spectroscopy and revealed the Si to Al ratio to be 13.24 to 7.96, showing the high purity of the extracted nanocomposite.
32099019	0	30	theme	Chinese	48:54	arg1	Ash					70:72	Incinerated Chinese Holy Joss Fly Ash	36:72	Incinerated Chinese Holy Joss Fly Ash	36:72	Aluminosilicate Nanocomposites from Incinerated Chinese Holy Joss Fly Ash: A Potential Nanocarrier for Drug Cargos.
32099019	1	31	theme	ash	258:260	arg1	waste					262:266	recycling joss fly ash waste	239:266	recycling joss fly ash waste	239:266	An incredible amount of joss fly ash is produced from the burning of Chinese holy joss paper; thus, an excellent method of recycling joss fly ash waste to extract aluminosilicate nanocomposites is explored.
32099019	12	32	theme	antibacterial	1954:1966	arg1	analysis					1968:1975	an antibacterial analysis	1951:1975	an antibacterial analysis against Escherichia coli (gram-negative) and Bacillus subtilis (gram-positive)	1951:2054	The drug delivery property was further justified through an antibacterial analysis against Escherichia coli (gram-negative) and Bacillus subtilis (gram-positive).
32099019	6	33	theme	particle	1090:1097	arg1	distribution					1099:1110	The roughness and particle distribution	1072:1110	The roughness and particle distribution	1072:1110	The roughness and particle distribution were analyzed using atomic force microscopy and a zeta analysis.
32099019	10	34	theme	nanocomposites	1644:1657	arg1	application					1613:1623	the promising application	1599:1623	the promising application of aluminosilicate nanocomposites as drug carriers	1599:1674	In addition, the research suggested the promising application of aluminosilicate nanocomposites as drug carriers.
32099019	11	35	theme	apta-sorbent	1721:1732	arg1	assay					1734:1738	an enzyme-linked apta-sorbent assay	1704:1738	an enzyme-linked apta-sorbent assay	1704:1738	The above was justified by an enzyme-linked apta-sorbent assay, which claimed that the limit of the aptasensing aluminosilicate-conjugated ampicillin was two-fold higher than that in the absence of the nanocomposite.
32099019	0	36	theme	Joss	61:64	arg1	Ash					70:72	Incinerated Chinese Holy Joss Fly Ash	36:72	Incinerated Chinese Holy Joss Fly Ash	36:72	Aluminosilicate Nanocomposites from Incinerated Chinese Holy Joss Fly Ash: A Potential Nanocarrier for Drug Cargos.
32099019	11	37	link	enzyme-linked	1707:1719	arg1	assay					1734:1738	an enzyme-linked apta-sorbent assay	1704:1738	an enzyme-linked apta-sorbent assay	1704:1738	The above was justified by an enzyme-linked apta-sorbent assay, which claimed that the limit of the aptasensing aluminosilicate-conjugated ampicillin was two-fold higher than that in the absence of the nanocomposite.
32099019	9	38	theme	thermal	1479:1485	arg1	capacity					1487:1494	The thermal capacity	1475:1494	The thermal capacity of the nanocomposite	1475:1515	The thermal capacity of the nanocomposite was examined by a thermogravimetric analysis.
32099019	4	39	theme	spherical-shaped	858:873	arg1	nanostructure					875:887	a uniformly spherical-shaped nanostructure	846:887	a uniformly spherical-shaped nanostructure	846:887	A morphological analysis was performed with field-emission transmission electron microscopy, and scanning electron microscopy revealed the size of the aluminosilicate nanocomposite to be ~25 nm, while also confirming a uniformly spherical-shaped nanostructure.
32099019	4	40	theme	electron	701:708	arg1	microscopy					710:719	field-emission transmission electron microscopy	673:719	field-emission transmission electron microscopy	673:719	A morphological analysis was performed with field-emission transmission electron microscopy, and scanning electron microscopy revealed the size of the aluminosilicate nanocomposite to be ~25 nm, while also confirming a uniformly spherical-shaped nanostructure.
32099019	5	41	theme	nanocomposite	1057:1069	arg1	purity					1033:1038	the high purity	1024:1038	the high purity of the extracted nanocomposite	1024:1069	The elemental composition was measured by energy dispersive spectroscopy and revealed the Si to Al ratio to be 13.24 to 7.96, showing the high purity of the extracted nanocomposite.
32099019	6	42	theme	zeta	1162:1165	arg1	analysis					1167:1174	a zeta analysis	1160:1174	a zeta analysis	1160:1174	The roughness and particle distribution were analyzed using atomic force microscopy and a zeta analysis.
32099019	10	43	theme	drug	1662:1665	arg1	carriers					1667:1674	drug carriers	1662:1674	drug carriers	1662:1674	In addition, the research suggested the promising application of aluminosilicate nanocomposites as drug carriers.
32099019	7	44	theme	diffraction	1183:1193	arg1	patterns					1195:1202	X-ray diffraction patterns	1177:1202	X-ray diffraction patterns	1177:1202	X-ray diffraction patterns showed a synthesis of faceted and cubic aluminosilicate crystals in the nanocomposites.
32099019	9	45	theme	nanocomposite	1503:1515	arg1	capacity					1487:1494	The thermal capacity	1475:1494	The thermal capacity of the nanocomposite	1475:1515	The thermal capacity of the nanocomposite was examined by a thermogravimetric analysis.
32099019	12	46	theme	drug	1898:1901	arg1	delivery					1903:1910	The drug delivery	1894:1910	The drug delivery property	1894:1919	The drug delivery property was further justified through an antibacterial analysis against Escherichia coli (gram-negative) and Bacillus subtilis (gram-positive).
32099019	12	47	dep	subtilis	2031:2038	arg1	gram-positive					2041:2053	gram-positive	2041:2053	gram-positive	2041:2053	The drug delivery property was further justified through an antibacterial analysis against Escherichia coli (gram-negative) and Bacillus subtilis (gram-positive).
32099019	1	48	theme	excellent	219:227	arg1	method					229:234	an excellent method	216:234	an excellent method of recycling joss fly ash waste to extract aluminosilicate nanocomposites	216:308	An incredible amount of joss fly ash is produced from the burning of Chinese holy joss paper; thus, an excellent method of recycling joss fly ash waste to extract aluminosilicate nanocomposites is explored.
32099019	11	49	theme	ampicillin	1816:1825	arg1	limit					1764:1768	the limit	1760:1768	the limit of the aptasensing aluminosilicate-conjugated ampicillin	1760:1825	The above was justified by an enzyme-linked apta-sorbent assay, which claimed that the limit of the aptasensing aluminosilicate-conjugated ampicillin was two-fold higher than that in the absence of the nanocomposite.
32099019	11	49	theme	ampicillin	1816:1825	arg1	higher					1840:1845	higher	1840:1845	higher	1840:1845	The above was justified by an enzyme-linked apta-sorbent assay, which claimed that the limit of the aptasensing aluminosilicate-conjugated ampicillin was two-fold higher than that in the absence of the nanocomposite.
32099019	3	50	theme	aluminosilicate	519:533	arg1	nanocomposite					535:547	The synthesized aluminosilicate nanocomposite	503:547	The synthesized aluminosilicate nanocomposite	503:547	The synthesized aluminosilicate nanocomposite was characterized to justify its structural and physiochemical characteristics.
32099019	5	51	theme	energy	932:937	arg1	spectroscopy					950:961	energy dispersive spectroscopy	932:961	energy dispersive spectroscopy	932:961	The elemental composition was measured by energy dispersive spectroscopy and revealed the Si to Al ratio to be 13.24 to 7.96, showing the high purity of the extracted nanocomposite.
32099019	8	52	theme	silica	1308:1313	arg1	presence					1296:1303	The presence	1292:1303	The presence of silica and aluminum	1292:1326	The presence of silica and aluminum was further proven by X-ray photoelectron spectroscopy, and the functional groups were recognized through Fourier transform infrared spectroscopy.
32099019	11	53	theme	aptasensing	1777:1787	arg1	ampicillin					1816:1825	the aptasensing aluminosilicate-conjugated ampicillin	1773:1825	the aptasensing aluminosilicate-conjugated ampicillin	1773:1825	The above was justified by an enzyme-linked apta-sorbent assay, which claimed that the limit of the aptasensing aluminosilicate-conjugated ampicillin was two-fold higher than that in the absence of the nanocomposite.
32099019	8	54	theme	aluminum	1319:1326	arg1	presence					1296:1303	The presence	1292:1303	The presence of silica and aluminum	1292:1326	The presence of silica and aluminum was further proven by X-ray photoelectron spectroscopy, and the functional groups were recognized through Fourier transform infrared spectroscopy.
32099019	1	55	theme	joss	198:201	arg1	paper					203:207	Chinese holy joss paper	185:207	Chinese holy joss paper	185:207	An incredible amount of joss fly ash is produced from the burning of Chinese holy joss paper; thus, an excellent method of recycling joss fly ash waste to extract aluminosilicate nanocomposites is explored.
32099019	8	56	dep	transform	1442:1450	arg1	infrared					1452:1459	infrared	1452:1459	transform infrared spectroscopy	1442:1472	The presence of silica and aluminum was further proven by X-ray photoelectron spectroscopy, and the functional groups were recognized through Fourier transform infrared spectroscopy.
32099019	1	57	theme	Chinese	185:191	arg1	paper					203:207	Chinese holy joss paper	185:207	Chinese holy joss paper	185:207	An incredible amount of joss fly ash is produced from the burning of Chinese holy joss paper; thus, an excellent method of recycling joss fly ash waste to extract aluminosilicate nanocomposites is explored.
32099019	4	58	theme	field-emission	673:686	arg1	microscopy					710:719	field-emission transmission electron microscopy	673:719	field-emission transmission electron microscopy	673:719	A morphological analysis was performed with field-emission transmission electron microscopy, and scanning electron microscopy revealed the size of the aluminosilicate nanocomposite to be ~25 nm, while also confirming a uniformly spherical-shaped nanostructure.
32099019	5	59	theme	Al	986:987	arg1	ratio					989:993	Al ratio	986:993	Al ratio	986:993	The elemental composition was measured by energy dispersive spectroscopy and revealed the Si to Al ratio to be 13.24 to 7.96, showing the high purity of the extracted nanocomposite.
32099019	8	60	theme	photoelectron	1356:1368	arg1	spectroscopy					1370:1381	X-ray photoelectron spectroscopy	1350:1381	X-ray photoelectron spectroscopy	1350:1381	The presence of silica and aluminum was further proven by X-ray photoelectron spectroscopy, and the functional groups were recognized through Fourier transform infrared spectroscopy.
32099019	1	61	theme	incredible	119:128	arg1	amount					130:135	An incredible amount	116:135	An incredible amount of joss fly ash	116:151	An incredible amount of joss fly ash is produced from the burning of Chinese holy joss paper; thus, an excellent method of recycling joss fly ash waste to extract aluminosilicate nanocomposites is explored.
32099019	1	61	theme	incredible	119:128	arg1	ash					149:151	joss fly ash	140:151	joss fly ash	140:151	An incredible amount of joss fly ash is produced from the burning of Chinese holy joss paper; thus, an excellent method of recycling joss fly ash waste to extract aluminosilicate nanocomposites is explored.
32099019	4	62	theme	scanning	726:733	arg1	microscopy					744:753	scanning electron microscopy	726:753	scanning electron microscopy	726:753	A morphological analysis was performed with field-emission transmission electron microscopy, and scanning electron microscopy revealed the size of the aluminosilicate nanocomposite to be ~25 nm, while also confirming a uniformly spherical-shaped nanostructure.
32099019	0	63	theme	Potential	77:85	arg1	Nanocarrier					87:97	A Potential Nanocarrier	75:97	Aluminosilicate Nanocomposites from Incinerated Chinese Holy Joss Fly Ash: A Potential Nanocarrier for Drug Cargos.	0:114	Aluminosilicate Nanocomposites from Incinerated Chinese Holy Joss Fly Ash: A Potential Nanocarrier for Drug Cargos.
32099019	1	64	theme	fly	145:147	arg1	ash					149:151	joss fly ash	140:151	joss fly ash	140:151	An incredible amount of joss fly ash is produced from the burning of Chinese holy joss paper; thus, an excellent method of recycling joss fly ash waste to extract aluminosilicate nanocomposites is explored.
32099019	7	65	from	synthesis	1213:1221	arg1	nanocomposites					1276:1289	the nanocomposites	1272:1289	the nanocomposites	1272:1289	X-ray diffraction patterns showed a synthesis of faceted and cubic aluminosilicate crystals in the nanocomposites.
32099019	7	66	theme	crystals	1260:1267	arg1	synthesis					1213:1221	a synthesis	1211:1221	a synthesis of faceted and cubic aluminosilicate crystals in the nanocomposites	1211:1289	X-ray diffraction patterns showed a synthesis of faceted and cubic aluminosilicate crystals in the nanocomposites.
32099019	5	67	theme	high	1028:1031	arg1	purity					1033:1038	the high purity	1024:1038	the high purity of the extracted nanocomposite	1024:1069	The elemental composition was measured by energy dispersive spectroscopy and revealed the Si to Al ratio to be 13.24 to 7.96, showing the high purity of the extracted nanocomposite.
32099019	7	68	theme	cubic	1238:1242	arg1	crystals					1260:1267	faceted and cubic aluminosilicate crystals	1226:1267	faceted and cubic aluminosilicate crystals	1226:1267	X-ray diffraction patterns showed a synthesis of faceted and cubic aluminosilicate crystals in the nanocomposites.
32099019	6	69	theme	atomic	1132:1137	arg1	microscopy					1145:1154	atomic force microscopy	1132:1154	atomic force microscopy	1132:1154	The roughness and particle distribution were analyzed using atomic force microscopy and a zeta analysis.
32099019	4	70	theme	aluminosilicate	780:794	arg1	nanocomposite					796:808	the aluminosilicate nanocomposite	776:808	the aluminosilicate nanocomposite	776:808	A morphological analysis was performed with field-emission transmission electron microscopy, and scanning electron microscopy revealed the size of the aluminosilicate nanocomposite to be ~25 nm, while also confirming a uniformly spherical-shaped nanostructure.
32099019	7	71	theme	faceted	1226:1232	arg1	crystals					1260:1267	faceted and cubic aluminosilicate crystals	1226:1267	faceted and cubic aluminosilicate crystals	1226:1267	X-ray diffraction patterns showed a synthesis of faceted and cubic aluminosilicate crystals in the nanocomposites.
32099019	1	72	theme	recycling	239:247	arg1	ash					258:260	recycling joss fly ash	239:260	recycling joss fly ash waste	239:266	An incredible amount of joss fly ash is produced from the burning of Chinese holy joss paper; thus, an excellent method of recycling joss fly ash waste to extract aluminosilicate nanocomposites is explored.
32099019	2	73	theme	joss	388:391	arg1	ash					397:399	joss fly ash	388:399	joss fly ash	388:399	The present research aims to introduce a novel method to recycle joss fly ash through a simple and straightforward experimental procedure involving acidic and alkaline treatments.
32099019	5	74	theme	dispersive	939:948	arg1	spectroscopy					950:961	energy dispersive spectroscopy	932:961	energy dispersive spectroscopy	932:961	The elemental composition was measured by energy dispersive spectroscopy and revealed the Si to Al ratio to be 13.24 to 7.96, showing the high purity of the extracted nanocomposite.
32099019	0	75	theme	Holy	56:59	arg1	Ash					70:72	Incinerated Chinese Holy Joss Fly Ash	36:72	Incinerated Chinese Holy Joss Fly Ash	36:72	Aluminosilicate Nanocomposites from Incinerated Chinese Holy Joss Fly Ash: A Potential Nanocarrier for Drug Cargos.
32099019	1	76	theme	fly	254:256	arg1	ash					258:260	recycling joss fly ash	239:260	recycling joss fly ash waste	239:266	An incredible amount of joss fly ash is produced from the burning of Chinese holy joss paper; thus, an excellent method of recycling joss fly ash waste to extract aluminosilicate nanocomposites is explored.
32099019	8	77	theme	X-ray	1350:1354	arg1	spectroscopy					1370:1381	X-ray photoelectron spectroscopy	1350:1381	X-ray photoelectron spectroscopy	1350:1381	The presence of silica and aluminum was further proven by X-ray photoelectron spectroscopy, and the functional groups were recognized through Fourier transform infrared spectroscopy.
32099019	4	78	theme	electron	735:742	arg1	microscopy					744:753	scanning electron microscopy	726:753	scanning electron microscopy	726:753	A morphological analysis was performed with field-emission transmission electron microscopy, and scanning electron microscopy revealed the size of the aluminosilicate nanocomposite to be ~25 nm, while also confirming a uniformly spherical-shaped nanostructure.
32099019	11	79	theme	enzyme-linked	1707:1719	arg1	assay					1734:1738	an enzyme-linked apta-sorbent assay	1704:1738	an enzyme-linked apta-sorbent assay	1704:1738	The above was justified by an enzyme-linked apta-sorbent assay, which claimed that the limit of the aptasensing aluminosilicate-conjugated ampicillin was two-fold higher than that in the absence of the nanocomposite.
32099019	2	80	theme	novel	364:368	arg1	method					370:375	a novel method	362:375	a novel method to recycle joss fly ash through a simple and straightforward experimental procedure involving acidic and alkaline treatments	362:500	The present research aims to introduce a novel method to recycle joss fly ash through a simple and straightforward experimental procedure involving acidic and alkaline treatments.
32099019	0	81	theme	Fly	66:68	arg1	Ash					70:72	Incinerated Chinese Holy Joss Fly Ash	36:72	Incinerated Chinese Holy Joss Fly Ash	36:72	Aluminosilicate Nanocomposites from Incinerated Chinese Holy Joss Fly Ash: A Potential Nanocarrier for Drug Cargos.
32099019	1	82	theme	waste	262:266	arg1	method					229:234	an excellent method	216:234	an excellent method of recycling joss fly ash waste to extract aluminosilicate nanocomposites	216:308	An incredible amount of joss fly ash is produced from the burning of Chinese holy joss paper; thus, an excellent method of recycling joss fly ash waste to extract aluminosilicate nanocomposites is explored.
32099019	10	83	theme	aluminosilicate	1628:1642	arg1	nanocomposites					1644:1657	aluminosilicate nanocomposites	1628:1657	aluminosilicate nanocomposites	1628:1657	In addition, the research suggested the promising application of aluminosilicate nanocomposites as drug carriers.
32099019	1	84	theme	extract	271:277	arg1	nanocomposites					295:308	extract aluminosilicate nanocomposites	271:308	extract aluminosilicate nanocomposites	271:308	An incredible amount of joss fly ash is produced from the burning of Chinese holy joss paper; thus, an excellent method of recycling joss fly ash waste to extract aluminosilicate nanocomposites is explored.
32099019	4	85	theme	morphological	631:643	arg1	analysis					645:652	A morphological analysis	629:652	A morphological analysis	629:652	A morphological analysis was performed with field-emission transmission electron microscopy, and scanning electron microscopy revealed the size of the aluminosilicate nanocomposite to be ~25 nm, while also confirming a uniformly spherical-shaped nanostructure.
32099019	2	86	theme	present	327:333	arg1	research					335:342	The present research	323:342	The present research	323:342	The present research aims to introduce a novel method to recycle joss fly ash through a simple and straightforward experimental procedure involving acidic and alkaline treatments.
32099019	1	87	theme	paper	203:207	arg1	burning					174:180	the burning	170:180	the burning of Chinese holy joss paper	170:207	An incredible amount of joss fly ash is produced from the burning of Chinese holy joss paper; thus, an excellent method of recycling joss fly ash waste to extract aluminosilicate nanocomposites is explored.
32099019	5	88	theme	extracted	1047:1055	arg1	nanocomposite					1057:1069	the extracted nanocomposite	1043:1069	the extracted nanocomposite	1043:1069	The elemental composition was measured by energy dispersive spectroscopy and revealed the Si to Al ratio to be 13.24 to 7.96, showing the high purity of the extracted nanocomposite.
32099019	3	89	theme	synthesized	507:517	arg1	nanocomposite					535:547	The synthesized aluminosilicate nanocomposite	503:547	The synthesized aluminosilicate nanocomposite	503:547	The synthesized aluminosilicate nanocomposite was characterized to justify its structural and physiochemical characteristics.
33948929	10	0	dep	source	1252:1257	arg1	fungal					1271:1276	fungal	1271:1276	fungal	1271:1276	Chitosan source (animal vs. fungal), co-polymerisation with gelatine and cross-linking using GPTMS or genipin had a significant effect on scaffold properties and hDPSCs response.
33948929	10	0	dep	source	1252:1257	arg1	Chitosan					1243:1250	Chitosan	1243:1250	Chitosan	1243:1250	Chitosan source (animal vs. fungal), co-polymerisation with gelatine and cross-linking using GPTMS or genipin had a significant effect on scaffold properties and hDPSCs response.
33948929	10	0	dep	source	1252:1257	arg1	animal					1260:1265	animal	1260:1265	animal	1260:1265	Chitosan source (animal vs. fungal), co-polymerisation with gelatine and cross-linking using GPTMS or genipin had a significant effect on scaffold properties and hDPSCs response.
33948929	3	1	theme	nano-focus	529:538	arg1	nano-CT					561:567	nano-CT	561:567	nano-CT	561:567	Scanning electron microscopy (SEM) and nano-focus computed tomography (nano-CT) were used to characterise scaffold microstructure.
33948929	3	1	theme	nano-focus	529:538	arg1	tomography					549:558	nano-focus computed tomography	529:558	nano-focus computed tomography (nano-CT)	529:568	Scanning electron microscopy (SEM) and nano-focus computed tomography (nano-CT) were used to characterise scaffold microstructure.
33948929	12	2	theme	chitosan-gelatine	1573:1589	arg1	co-polymerisation					1591:1607	Animal chitosan-gelatine co-polymerisation	1566:1607	Animal chitosan-gelatine co-polymerisation	1566:1607	Animal chitosan-gelatine co-polymerisation increased scaffold compressive strength, while fungal chitosan scaffolds (fCS-GEL-GPTMS) had the fastest degradation rate, losing 80 % of their weight by day 21.
33948929	8	3	theme	human	1089:1093	arg1	hDPSCs					1119:1124	hDPSCs	1119:1124	hDPSCs	1119:1124	Finally, human dental pulp stem cells (hDPSCs) viability was evaluated.
33948929	8	3	theme	human	1089:1093	arg1	cells					1112:1116	human dental pulp stem cells	1089:1116	human dental pulp stem cells (hDPSCs) viability	1089:1135	Finally, human dental pulp stem cells (hDPSCs) viability was evaluated.
33948929	4	4	theme	reflectance	722:732	arg1	spectroscopy					734:745	infrared-attenuated total reflectance spectroscopy	696:745	infrared-attenuated total reflectance spectroscopy	696:745	Chemical composition and cross-linking were evaluated by Fourier transform infrared-attenuated total reflectance spectroscopy.
33948929	8	5	theme	pulp	1102:1105	arg1	hDPSCs					1119:1124	hDPSCs	1119:1124	hDPSCs	1119:1124	Finally, human dental pulp stem cells (hDPSCs) viability was evaluated.
33948929	8	5	theme	pulp	1102:1105	arg1	cells					1112:1116	human dental pulp stem cells	1089:1116	human dental pulp stem cells (hDPSCs) viability	1089:1135	Finally, human dental pulp stem cells (hDPSCs) viability was evaluated.
33948929	12	6	contain	had	1698:1700	arg1	fCS-GEL-GPTMS					1683:1695	fCS-GEL-GPTMS	1683:1695	fCS-GEL-GPTMS	1683:1695	Animal chitosan-gelatine co-polymerisation increased scaffold compressive strength, while fungal chitosan scaffolds (fCS-GEL-GPTMS) had the fastest degradation rate, losing 80 % of their weight by day 21.
33948929	12	6	contain	had	1698:1700	arg2	rate					1726:1729	the fastest degradation rate	1702:1729	the fastest degradation rate	1702:1729	Animal chitosan-gelatine co-polymerisation increased scaffold compressive strength, while fungal chitosan scaffolds (fCS-GEL-GPTMS) had the fastest degradation rate, losing 80 % of their weight by day 21.
33948929	12	6	contain	had	1698:1700	arg1	scaffolds					1672:1680	fungal chitosan scaffolds	1656:1680	fungal chitosan scaffolds (fCS-GEL-GPTMS)	1656:1696	Animal chitosan-gelatine co-polymerisation increased scaffold compressive strength, while fungal chitosan scaffolds (fCS-GEL-GPTMS) had the fastest degradation rate, losing 80 % of their weight by day 21.
33948929	3	7	theme	scaffold	596:603	arg1	microstructure					605:618	scaffold microstructure	596:618	scaffold microstructure	596:618	Scanning electron microscopy (SEM) and nano-focus computed tomography (nano-CT) were used to characterise scaffold microstructure.
33948929	14	8	theme	dentistry	2071:2079	arg1	applications					2081:2092	regenerative dentistry applications	2058:2092	regenerative dentistry applications	2058:2092	Tailored chitosan scaffolds with tuned properties and favourable hDPSCs response can be obtained for regenerative dentistry applications.
33948929	8	9	theme	cells	1112:1116	arg1	viability					1127:1135	human dental pulp stem cells (hDPSCs) viability	1089:1135	human dental pulp stem cells (hDPSCs) viability	1089:1135	Finally, human dental pulp stem cells (hDPSCs) viability was evaluated.
33948929	10	10	from	effect	1371:1376	arg1	properties					1390:1399	scaffold properties	1381:1399	scaffold properties	1381:1399	Chitosan source (animal vs. fungal), co-polymerisation with gelatine and cross-linking using GPTMS or genipin had a significant effect on scaffold properties and hDPSCs response.
33948929	13	11	theme	improved	1915:1922	arg1	attachment					1931:1940	improved hDPSCs attachment	1915:1940	improved hDPSCs attachment	1915:1940	Gelatine co-polymerisation and GPTMS cross-linking enhanced chitosan scaffolds bioactivity through the formation of an apatite layer as well as improved hDPSCs attachment and viability.
33948929	1	12	theme	root	385:388	arg1	engineering					397:407	dental root tissue engineering	378:407	dental root tissue engineering	378:407	The aim of the present study was to fabricate and characterise chitosan scaffolds from animal and fungal sources, with or without gelatine as a co-polymer, and cross-linked to 3-glycidyloxyproply trimethoxysilane (GPTMS) or genipin for application in dental root tissue engineering.
33948929	7	13	theme	simulated	924:932	arg1	fluid					939:943	simulated body fluid	924:943	simulated body fluid	924:943	Scaffold bioactivity immersed in simulated body fluid was evaluated by SEM, with associated electron dispersive X-ray spectroscopy, and apatite formation was examined by X-ray diffraction.
33948929	1	14	theme	animal	214:219	arg1	sources					232:238	animal and fungal sources	214:238	animal and fungal sources	214:238	The aim of the present study was to fabricate and characterise chitosan scaffolds from animal and fungal sources, with or without gelatine as a co-polymer, and cross-linked to 3-glycidyloxyproply trimethoxysilane (GPTMS) or genipin for application in dental root tissue engineering.
33948929	4	15	theme	infrared-attenuated	696:714	arg1	spectroscopy					734:745	infrared-attenuated total reflectance spectroscopy	696:745	infrared-attenuated total reflectance spectroscopy	696:745	Chemical composition and cross-linking were evaluated by Fourier transform infrared-attenuated total reflectance spectroscopy.
33948929	11	16	theme	pore	1474:1477	arg1	diameter					1479:1486	the largest pore diameter	1462:1486	the largest pore diameter	1462:1486	Chitosan-genipin (CS-GEN) scaffolds had the largest pore diameter, while the chitosan-gelatine-GPTMS (CS-GEL-GPTMS) scaffolds had the smallest.
33948929	5	17	theme	mechanical	802:811	arg1	properties					813:822	scaffold mechanical properties	793:822	scaffold mechanical properties	793:822	Compression tests were performed to evaluate scaffold mechanical properties.
33948929	1	18	theme	fungal	225:230	arg1	sources					232:238	animal and fungal sources	214:238	animal and fungal sources	214:238	The aim of the present study was to fabricate and characterise chitosan scaffolds from animal and fungal sources, with or without gelatine as a co-polymer, and cross-linked to 3-glycidyloxyproply trimethoxysilane (GPTMS) or genipin for application in dental root tissue engineering.
33948929	13	19	theme	layer	1898:1902	arg1	viability					1946:1954	viability	1946:1954	viability	1946:1954	Gelatine co-polymerisation and GPTMS cross-linking enhanced chitosan scaffolds bioactivity through the formation of an apatite layer as well as improved hDPSCs attachment and viability.
33948929	13	19	theme	layer	1898:1902	arg1	attachment					1931:1940	improved hDPSCs attachment	1915:1940	improved hDPSCs attachment	1915:1940	Gelatine co-polymerisation and GPTMS cross-linking enhanced chitosan scaffolds bioactivity through the formation of an apatite layer as well as improved hDPSCs attachment and viability.
33948929	13	19	theme	layer	1898:1902	arg1	formation					1874:1882	the formation	1870:1882	the formation of an apatite layer as well as improved hDPSCs attachment and viability	1870:1954	Gelatine co-polymerisation and GPTMS cross-linking enhanced chitosan scaffolds bioactivity through the formation of an apatite layer as well as improved hDPSCs attachment and viability.
33948929	10	20	contain	had	1353:1355	arg2	effect					1371:1376	a significant effect	1357:1376	a significant effect on scaffold properties	1357:1399	Chitosan source (animal vs. fungal), co-polymerisation with gelatine and cross-linking using GPTMS or genipin had a significant effect on scaffold properties and hDPSCs response.
33948929	10	20	contain	had	1353:1355	arg2	response					1412:1419	hDPSCs response	1405:1419	hDPSCs response	1405:1419	Chitosan source (animal vs. fungal), co-polymerisation with gelatine and cross-linking using GPTMS or genipin had a significant effect on scaffold properties and hDPSCs response.
33948929	10	20	contain	had	1353:1355	arg1	co-polymerisation					1280:1296	co-polymerisation	1280:1296	co-polymerisation	1280:1296	Chitosan source (animal vs. fungal), co-polymerisation with gelatine and cross-linking using GPTMS or genipin had a significant effect on scaffold properties and hDPSCs response.
33948929	10	20	contain	had	1353:1355	arg1	source					1252:1257	source	1252:1257	source	1252:1257	Chitosan source (animal vs. fungal), co-polymerisation with gelatine and cross-linking using GPTMS or genipin had a significant effect on scaffold properties and hDPSCs response.
33948929	10	20	contain	had	1353:1355	arg1	cross-linking					1316:1328	cross-linking	1316:1328	cross-linking	1316:1328	Chitosan source (animal vs. fungal), co-polymerisation with gelatine and cross-linking using GPTMS or genipin had a significant effect on scaffold properties and hDPSCs response.
33948929	10	21	from	response	1412:1419	arg1	properties					1390:1399	scaffold properties	1381:1399	scaffold properties	1381:1399	Chitosan source (animal vs. fungal), co-polymerisation with gelatine and cross-linking using GPTMS or genipin had a significant effect on scaffold properties and hDPSCs response.
33948929	1	22	theme	present	142:148	arg1	study					150:154	the present study	138:154	the present study	138:154	The aim of the present study was to fabricate and characterise chitosan scaffolds from animal and fungal sources, with or without gelatine as a co-polymer, and cross-linked to 3-glycidyloxyproply trimethoxysilane (GPTMS) or genipin for application in dental root tissue engineering.
33948929	12	23	theme	fastest	1706:1712	arg1	rate					1726:1729	the fastest degradation rate	1702:1729	the fastest degradation rate	1702:1729	Animal chitosan-gelatine co-polymerisation increased scaffold compressive strength, while fungal chitosan scaffolds (fCS-GEL-GPTMS) had the fastest degradation rate, losing 80 % of their weight by day 21.
33948929	14	24	theme	Tailored	1957:1964	arg1	scaffolds					1975:1983	Tailored chitosan scaffolds	1957:1983	Tailored chitosan scaffolds with tuned properties and favourable hDPSCs response	1957:2036	Tailored chitosan scaffolds with tuned properties and favourable hDPSCs response can be obtained for regenerative dentistry applications.
33948929	10	25	theme	significant	1359:1369	arg1	effect					1371:1376	a significant effect	1357:1376	a significant effect on scaffold properties	1357:1399	Chitosan source (animal vs. fungal), co-polymerisation with gelatine and cross-linking using GPTMS or genipin had a significant effect on scaffold properties and hDPSCs response.
33948929	9	26	theme	fabrication	1156:1166	arg1	method					1168:1173	The fabrication method	1152:1173	The fabrication method used	1152:1178	The fabrication method used was successful in producing scaffolds with organised porosity.
33948929	9	26	theme	fabrication	1156:1166	arg1	successful					1184:1193	successful	1184:1193	successful	1184:1193	The fabrication method used was successful in producing scaffolds with organised porosity.
33948929	0	27	theme	tissue	107:112	arg1	engineering					114:124	dental tissue engineering	100:124	dental tissue engineering	100:124	3D-printing-assisted fabrication of chitosan scaffolds from different sources and cross-linkers for dental tissue engineering.
33948929	14	28	theme	favourable	2011:2020	arg1	response					2029:2036	favourable hDPSCs response	2011:2036	favourable hDPSCs response	2011:2036	Tailored chitosan scaffolds with tuned properties and favourable hDPSCs response can be obtained for regenerative dentistry applications.
33948929	10	29	with	co-polymerisation	1280:1296	arg1	gelatine					1303:1310	gelatine	1303:1310	gelatine	1303:1310	Chitosan source (animal vs. fungal), co-polymerisation with gelatine and cross-linking using GPTMS or genipin had a significant effect on scaffold properties and hDPSCs response.
33948929	2	30	theme	freeze-drying	465:477	arg1	technique					479:487	the emulsion freeze-drying technique	452:487	the emulsion freeze-drying technique	452:487	Chitosan-based scaffolds were prepared by the emulsion freeze-drying technique.
33948929	0	31	theme	3D-printing-assisted	0:19	arg1	fabrication					21:31	3D-printing-assisted fabrication	0:31	3D-printing-assisted fabrication of chitosan	0:43	3D-printing-assisted fabrication of chitosan scaffolds from different sources and cross-linkers for dental tissue engineering.
33948929	7	32	theme	apatite	1027:1033	arg1	formation					1035:1043	apatite formation	1027:1043	apatite formation	1027:1043	Scaffold bioactivity immersed in simulated body fluid was evaluated by SEM, with associated electron dispersive X-ray spectroscopy, and apatite formation was examined by X-ray diffraction.
33948929	13	33	theme	Gelatine	1771:1778	arg1	co-polymerisation					1780:1796	Gelatine co-polymerisation	1771:1796	Gelatine co-polymerisation	1771:1796	Gelatine co-polymerisation and GPTMS cross-linking enhanced chitosan scaffolds bioactivity through the formation of an apatite layer as well as improved hDPSCs attachment and viability.
33948929	3	34	theme	Scanning	490:497	arg1	SEM					520:522	SEM	520:522	SEM	520:522	Scanning electron microscopy (SEM) and nano-focus computed tomography (nano-CT) were used to characterise scaffold microstructure.
33948929	3	34	theme	Scanning	490:497	arg1	microscopy					508:517	Scanning electron microscopy	490:517	Scanning electron microscopy (SEM)	490:523	Scanning electron microscopy (SEM) and nano-focus computed tomography (nano-CT) were used to characterise scaffold microstructure.
33948929	1	35	from	application	363:373	arg1	engineering					397:407	dental root tissue engineering	378:407	dental root tissue engineering	378:407	The aim of the present study was to fabricate and characterise chitosan scaffolds from animal and fungal sources, with or without gelatine as a co-polymer, and cross-linked to 3-glycidyloxyproply trimethoxysilane (GPTMS) or genipin for application in dental root tissue engineering.
33948929	7	36	theme	Scaffold	891:898	arg1	bioactivity					900:910	Scaffold bioactivity	891:910	Scaffold bioactivity immersed in simulated body fluid	891:943	Scaffold bioactivity immersed in simulated body fluid was evaluated by SEM, with associated electron dispersive X-ray spectroscopy, and apatite formation was examined by X-ray diffraction.
33948929	10	37	theme	hDPSCs	1405:1410	arg1	response					1412:1419	hDPSCs response	1405:1419	hDPSCs response	1405:1419	Chitosan source (animal vs. fungal), co-polymerisation with gelatine and cross-linking using GPTMS or genipin had a significant effect on scaffold properties and hDPSCs response.
33948929	12	38	theme	chitosan	1663:1670	arg1	fCS-GEL-GPTMS					1683:1695	fCS-GEL-GPTMS	1683:1695	fCS-GEL-GPTMS	1683:1695	Animal chitosan-gelatine co-polymerisation increased scaffold compressive strength, while fungal chitosan scaffolds (fCS-GEL-GPTMS) had the fastest degradation rate, losing 80 % of their weight by day 21.
33948929	12	38	theme	chitosan	1663:1670	arg1	scaffolds					1672:1680	fungal chitosan scaffolds	1656:1680	fungal chitosan scaffolds (fCS-GEL-GPTMS)	1656:1696	Animal chitosan-gelatine co-polymerisation increased scaffold compressive strength, while fungal chitosan scaffolds (fCS-GEL-GPTMS) had the fastest degradation rate, losing 80 % of their weight by day 21.
33948929	0	39	theme	different	60:68	arg1	sources					70:76	different sources	60:76	different sources	60:76	3D-printing-assisted fabrication of chitosan scaffolds from different sources and cross-linkers for dental tissue engineering.
33948929	12	40	theme	scaffold	1619:1626	arg1	strength					1640:1647	scaffold compressive strength	1619:1647	scaffold compressive strength	1619:1647	Animal chitosan-gelatine co-polymerisation increased scaffold compressive strength, while fungal chitosan scaffolds (fCS-GEL-GPTMS) had the fastest degradation rate, losing 80 % of their weight by day 21.
33948929	9	41	theme	organised	1223:1231	arg1	porosity					1233:1240	organised porosity	1223:1240	organised porosity	1223:1240	The fabrication method used was successful in producing scaffolds with organised porosity.
33948929	6	42	theme	Scaffold	825:832	arg1	degradation					834:844	Scaffold degradation	825:844	Scaffold degradation	825:844	Scaffold degradation was evaluated by gravimetric method and SEM.
33948929	3	43	theme	computed	540:547	arg1	nano-CT					561:567	nano-CT	561:567	nano-CT	561:567	Scanning electron microscopy (SEM) and nano-focus computed tomography (nano-CT) were used to characterise scaffold microstructure.
33948929	3	43	theme	computed	540:547	arg1	tomography					549:558	nano-focus computed tomography	529:558	nano-focus computed tomography (nano-CT)	529:568	Scanning electron microscopy (SEM) and nano-focus computed tomography (nano-CT) were used to characterise scaffold microstructure.
33948929	12	44	theme	Animal	1566:1571	arg1	co-polymerisation					1591:1607	Animal chitosan-gelatine co-polymerisation	1566:1607	Animal chitosan-gelatine co-polymerisation	1566:1607	Animal chitosan-gelatine co-polymerisation increased scaffold compressive strength, while fungal chitosan scaffolds (fCS-GEL-GPTMS) had the fastest degradation rate, losing 80 % of their weight by day 21.
33948929	7	45	theme	dispersive	992:1001	arg1	spectroscopy					1009:1020	associated electron dispersive X-ray spectroscopy	972:1020	associated electron dispersive X-ray spectroscopy	972:1020	Scaffold bioactivity immersed in simulated body fluid was evaluated by SEM, with associated electron dispersive X-ray spectroscopy, and apatite formation was examined by X-ray diffraction.
33948929	13	46	dep	co-polymerisation	1780:1796	arg1	chitosan					1831:1838	enhanced chitosan	1822:1838	enhanced chitosan	1822:1838	Gelatine co-polymerisation and GPTMS cross-linking enhanced chitosan scaffolds bioactivity through the formation of an apatite layer as well as improved hDPSCs attachment and viability.
33948929	13	47	theme	hDPSCs	1924:1929	arg1	attachment					1931:1940	improved hDPSCs attachment	1915:1940	improved hDPSCs attachment	1915:1940	Gelatine co-polymerisation and GPTMS cross-linking enhanced chitosan scaffolds bioactivity through the formation of an apatite layer as well as improved hDPSCs attachment and viability.
33948929	7	48	theme	associated	972:981	arg1	spectroscopy					1009:1020	associated electron dispersive X-ray spectroscopy	972:1020	associated electron dispersive X-ray spectroscopy	972:1020	Scaffold bioactivity immersed in simulated body fluid was evaluated by SEM, with associated electron dispersive X-ray spectroscopy, and apatite formation was examined by X-ray diffraction.
33948929	11	49	contain	had	1548:1550	arg1	scaffolds					1538:1546	the chitosan-gelatine-GPTMS (CS-GEL-GPTMS) scaffolds	1495:1546	the chitosan-gelatine-GPTMS (CS-GEL-GPTMS) scaffolds	1495:1546	Chitosan-genipin (CS-GEN) scaffolds had the largest pore diameter, while the chitosan-gelatine-GPTMS (CS-GEL-GPTMS) scaffolds had the smallest.
33948929	11	49	contain	had	1548:1550	arg2	smallest					1556:1563	smallest	1556:1563	smallest	1556:1563	Chitosan-genipin (CS-GEN) scaffolds had the largest pore diameter, while the chitosan-gelatine-GPTMS (CS-GEL-GPTMS) scaffolds had the smallest.
33948929	8	50	theme	dental	1095:1100	arg1	hDPSCs					1119:1124	hDPSCs	1119:1124	hDPSCs	1119:1124	Finally, human dental pulp stem cells (hDPSCs) viability was evaluated.
33948929	8	50	theme	dental	1095:1100	arg1	cells					1112:1116	human dental pulp stem cells	1089:1116	human dental pulp stem cells (hDPSCs) viability	1089:1135	Finally, human dental pulp stem cells (hDPSCs) viability was evaluated.
33948929	4	51	theme	Chemical	621:628	arg1	composition					630:640	Chemical composition	621:640	Chemical composition	621:640	Chemical composition and cross-linking were evaluated by Fourier transform infrared-attenuated total reflectance spectroscopy.
33948929	11	52	theme	chitosan-gelatine-GPTMS	1499:1521	arg1	scaffolds					1538:1546	the chitosan-gelatine-GPTMS (CS-GEL-GPTMS) scaffolds	1495:1546	the chitosan-gelatine-GPTMS (CS-GEL-GPTMS) scaffolds	1495:1546	Chitosan-genipin (CS-GEN) scaffolds had the largest pore diameter, while the chitosan-gelatine-GPTMS (CS-GEL-GPTMS) scaffolds had the smallest.
33948929	14	53	with	scaffolds	1975:1983	arg1	properties					1996:2005	tuned properties	1990:2005	tuned properties	1990:2005	Tailored chitosan scaffolds with tuned properties and favourable hDPSCs response can be obtained for regenerative dentistry applications.
33948929	14	53	with	scaffolds	1975:1983	arg1	response					2029:2036	favourable hDPSCs response	2011:2036	favourable hDPSCs response	2011:2036	Tailored chitosan scaffolds with tuned properties and favourable hDPSCs response can be obtained for regenerative dentistry applications.
33948929	8	54	theme	stem	1107:1110	arg1	hDPSCs					1119:1124	hDPSCs	1119:1124	hDPSCs	1119:1124	Finally, human dental pulp stem cells (hDPSCs) viability was evaluated.
33948929	8	54	theme	stem	1107:1110	arg1	cells					1112:1116	human dental pulp stem cells	1089:1116	human dental pulp stem cells (hDPSCs) viability	1089:1135	Finally, human dental pulp stem cells (hDPSCs) viability was evaluated.
33948929	4	55	theme	total	716:720	arg1	spectroscopy					734:745	infrared-attenuated total reflectance spectroscopy	696:745	infrared-attenuated total reflectance spectroscopy	696:745	Chemical composition and cross-linking were evaluated by Fourier transform infrared-attenuated total reflectance spectroscopy.
33948929	1	56	theme	chitosan	190:197	arg1	co-polymer					271:280	a co-polymer	269:280	a co-polymer	269:280	The aim of the present study was to fabricate and characterise chitosan scaffolds from animal and fungal sources, with or without gelatine as a co-polymer, and cross-linked to 3-glycidyloxyproply trimethoxysilane (GPTMS) or genipin for application in dental root tissue engineering.
33948929	1	56	theme	chitosan	190:197	arg1	scaffolds					199:207	chitosan scaffolds	190:207	chitosan scaffolds	190:207	The aim of the present study was to fabricate and characterise chitosan scaffolds from animal and fungal sources, with or without gelatine as a co-polymer, and cross-linked to 3-glycidyloxyproply trimethoxysilane (GPTMS) or genipin for application in dental root tissue engineering.
33948929	14	57	theme	regenerative	2058:2069	arg1	applications					2081:2092	regenerative dentistry applications	2058:2092	regenerative dentistry applications	2058:2092	Tailored chitosan scaffolds with tuned properties and favourable hDPSCs response can be obtained for regenerative dentistry applications.
33948929	12	58	theme	weight	1753:1758	arg1	%					1742:1742	80 %	1739:1742	80 % of their weight	1739:1758	Animal chitosan-gelatine co-polymerisation increased scaffold compressive strength, while fungal chitosan scaffolds (fCS-GEL-GPTMS) had the fastest degradation rate, losing 80 % of their weight by day 21.
33948929	12	58	theme	weight	1753:1758	arg1	weight					1753:1758	their weight	1747:1758	their weight	1747:1758	Animal chitosan-gelatine co-polymerisation increased scaffold compressive strength, while fungal chitosan scaffolds (fCS-GEL-GPTMS) had the fastest degradation rate, losing 80 % of their weight by day 21.
33948929	3	59	used	used	575:578	arg2	tomography					549:558	nano-focus computed tomography	529:558	nano-focus computed tomography (nano-CT)	529:568	Scanning electron microscopy (SEM) and nano-focus computed tomography (nano-CT) were used to characterise scaffold microstructure.
33948929	3	59	used	used	575:578	arg2	microscopy					508:517	Scanning electron microscopy	490:517	Scanning electron microscopy (SEM)	490:523	Scanning electron microscopy (SEM) and nano-focus computed tomography (nano-CT) were used to characterise scaffold microstructure.
33948929	3	59	used	used	575:578	arg2	nano-CT					561:567	nano-CT	561:567	nano-CT	561:567	Scanning electron microscopy (SEM) and nano-focus computed tomography (nano-CT) were used to characterise scaffold microstructure.
33948929	3	59	used	used	575:578	arg2	SEM					520:522	SEM	520:522	SEM	520:522	Scanning electron microscopy (SEM) and nano-focus computed tomography (nano-CT) were used to characterise scaffold microstructure.
33948929	11	60	theme	largest	1466:1472	arg1	diameter					1479:1486	the largest pore diameter	1462:1486	the largest pore diameter	1462:1486	Chitosan-genipin (CS-GEN) scaffolds had the largest pore diameter, while the chitosan-gelatine-GPTMS (CS-GEL-GPTMS) scaffolds had the smallest.
33948929	5	61	theme	scaffold	793:800	arg1	properties					813:822	scaffold mechanical properties	793:822	scaffold mechanical properties	793:822	Compression tests were performed to evaluate scaffold mechanical properties.
33948929	1	62	theme	tissue	390:395	arg1	engineering					397:407	dental root tissue engineering	378:407	dental root tissue engineering	378:407	The aim of the present study was to fabricate and characterise chitosan scaffolds from animal and fungal sources, with or without gelatine as a co-polymer, and cross-linked to 3-glycidyloxyproply trimethoxysilane (GPTMS) or genipin for application in dental root tissue engineering.
33948929	7	63	theme	body	934:937	arg1	fluid					939:943	simulated body fluid	924:943	simulated body fluid	924:943	Scaffold bioactivity immersed in simulated body fluid was evaluated by SEM, with associated electron dispersive X-ray spectroscopy, and apatite formation was examined by X-ray diffraction.
33948929	11	64	contain	had	1458:1460	arg2	diameter					1479:1486	the largest pore diameter	1462:1486	the largest pore diameter	1462:1486	Chitosan-genipin (CS-GEN) scaffolds had the largest pore diameter, while the chitosan-gelatine-GPTMS (CS-GEL-GPTMS) scaffolds had the smallest.
33948929	11	64	contain	had	1458:1460	arg1	scaffolds					1448:1456	Chitosan-genipin (CS-GEN) scaffolds	1422:1456	Chitosan-genipin (CS-GEN) scaffolds	1422:1456	Chitosan-genipin (CS-GEN) scaffolds had the largest pore diameter, while the chitosan-gelatine-GPTMS (CS-GEL-GPTMS) scaffolds had the smallest.
33948929	13	65	theme	apatite	1890:1896	arg1	layer					1898:1902	an apatite layer	1887:1902	an apatite layer	1887:1902	Gelatine co-polymerisation and GPTMS cross-linking enhanced chitosan scaffolds bioactivity through the formation of an apatite layer as well as improved hDPSCs attachment and viability.
33948929	5	66	theme	Compression	748:758	arg1	tests					760:764	Compression tests	748:764	Compression tests	748:764	Compression tests were performed to evaluate scaffold mechanical properties.
33948929	1	67	from	sources	232:238	arg1	co-polymer					271:280	a co-polymer	269:280	a co-polymer	269:280	The aim of the present study was to fabricate and characterise chitosan scaffolds from animal and fungal sources, with or without gelatine as a co-polymer, and cross-linked to 3-glycidyloxyproply trimethoxysilane (GPTMS) or genipin for application in dental root tissue engineering.
33948929	1	67	from	sources	232:238	arg1	scaffolds					199:207	chitosan scaffolds	190:207	chitosan scaffolds	190:207	The aim of the present study was to fabricate and characterise chitosan scaffolds from animal and fungal sources, with or without gelatine as a co-polymer, and cross-linked to 3-glycidyloxyproply trimethoxysilane (GPTMS) or genipin for application in dental root tissue engineering.
33948929	14	68	theme	hDPSCs	2022:2027	arg1	response					2029:2036	favourable hDPSCs response	2011:2036	favourable hDPSCs response	2011:2036	Tailored chitosan scaffolds with tuned properties and favourable hDPSCs response can be obtained for regenerative dentistry applications.
33948929	1	69	theme	study	150:154	arg1	aim					131:133	The aim	127:133	The aim of the present study	127:154	The aim of the present study was to fabricate and characterise chitosan scaffolds from animal and fungal sources, with or without gelatine as a co-polymer, and cross-linked to 3-glycidyloxyproply trimethoxysilane (GPTMS) or genipin for application in dental root tissue engineering.
33948929	12	70	theme	degradation	1714:1724	arg1	rate					1726:1729	the fastest degradation rate	1702:1729	the fastest degradation rate	1702:1729	Animal chitosan-gelatine co-polymerisation increased scaffold compressive strength, while fungal chitosan scaffolds (fCS-GEL-GPTMS) had the fastest degradation rate, losing 80 % of their weight by day 21.
33948929	0	71	theme	dental	100:105	arg1	engineering					114:124	dental tissue engineering	100:124	dental tissue engineering	100:124	3D-printing-assisted fabrication of chitosan scaffolds from different sources and cross-linkers for dental tissue engineering.
33948929	14	72	theme	tuned	1990:1994	arg1	properties					1996:2005	tuned properties	1990:2005	tuned properties	1990:2005	Tailored chitosan scaffolds with tuned properties and favourable hDPSCs response can be obtained for regenerative dentistry applications.
33948929	7	73	theme	X-ray	1061:1065	arg1	diffraction					1067:1077	X-ray diffraction	1061:1077	X-ray diffraction	1061:1077	Scaffold bioactivity immersed in simulated body fluid was evaluated by SEM, with associated electron dispersive X-ray spectroscopy, and apatite formation was examined by X-ray diffraction.
33948929	11	74	theme	Chitosan-genipin	1422:1437	arg1	scaffolds					1448:1456	Chitosan-genipin (CS-GEN) scaffolds	1422:1456	Chitosan-genipin (CS-GEN) scaffolds	1422:1456	Chitosan-genipin (CS-GEN) scaffolds had the largest pore diameter, while the chitosan-gelatine-GPTMS (CS-GEL-GPTMS) scaffolds had the smallest.
33948929	10	75	theme	scaffold	1381:1388	arg1	properties					1390:1399	scaffold properties	1381:1399	scaffold properties	1381:1399	Chitosan source (animal vs. fungal), co-polymerisation with gelatine and cross-linking using GPTMS or genipin had a significant effect on scaffold properties and hDPSCs response.
33948929	1	76	theme	dental	378:383	arg1	engineering					397:407	dental root tissue engineering	378:407	dental root tissue engineering	378:407	The aim of the present study was to fabricate and characterise chitosan scaffolds from animal and fungal sources, with or without gelatine as a co-polymer, and cross-linked to 3-glycidyloxyproply trimethoxysilane (GPTMS) or genipin for application in dental root tissue engineering.
33948929	2	77	theme	emulsion	456:463	arg1	technique					479:487	the emulsion freeze-drying technique	452:487	the emulsion freeze-drying technique	452:487	Chitosan-based scaffolds were prepared by the emulsion freeze-drying technique.
33948929	0	78	theme	chitosan	36:43	arg1	fabrication					21:31	3D-printing-assisted fabrication	0:31	3D-printing-assisted fabrication of chitosan	0:43	3D-printing-assisted fabrication of chitosan scaffolds from different sources and cross-linkers for dental tissue engineering.
33948929	14	79	theme	chitosan	1966:1973	arg1	scaffolds					1975:1983	Tailored chitosan scaffolds	1957:1983	Tailored chitosan scaffolds with tuned properties and favourable hDPSCs response	1957:2036	Tailored chitosan scaffolds with tuned properties and favourable hDPSCs response can be obtained for regenerative dentistry applications.
33948929	3	80	theme	electron	499:506	arg1	SEM					520:522	SEM	520:522	SEM	520:522	Scanning electron microscopy (SEM) and nano-focus computed tomography (nano-CT) were used to characterise scaffold microstructure.
33948929	3	80	theme	electron	499:506	arg1	microscopy					508:517	Scanning electron microscopy	490:517	Scanning electron microscopy (SEM)	490:523	Scanning electron microscopy (SEM) and nano-focus computed tomography (nano-CT) were used to characterise scaffold microstructure.
33948929	13	81	theme	GPTMS	1802:1806	arg1	cross-linking					1808:1820	GPTMS cross-linking	1802:1820	GPTMS cross-linking	1802:1820	Gelatine co-polymerisation and GPTMS cross-linking enhanced chitosan scaffolds bioactivity through the formation of an apatite layer as well as improved hDPSCs attachment and viability.
33948929	6	82	theme	gravimetric	863:873	arg1	method					875:880	gravimetric method	863:880	gravimetric method	863:880	Scaffold degradation was evaluated by gravimetric method and SEM.
33948929	2	83	theme	Chitosan-based	410:423	arg1	scaffolds					425:433	Chitosan-based scaffolds	410:433	Chitosan-based scaffolds	410:433	Chitosan-based scaffolds were prepared by the emulsion freeze-drying technique.
33948929	1	84	theme	3-glycidyloxyproply	303:321	arg1	GPTMS					341:345	GPTMS	341:345	GPTMS	341:345	The aim of the present study was to fabricate and characterise chitosan scaffolds from animal and fungal sources, with or without gelatine as a co-polymer, and cross-linked to 3-glycidyloxyproply trimethoxysilane (GPTMS) or genipin for application in dental root tissue engineering.
33948929	1	84	theme	3-glycidyloxyproply	303:321	arg1	trimethoxysilane					323:338	3-glycidyloxyproply trimethoxysilane	303:338	3-glycidyloxyproply trimethoxysilane (GPTMS)	303:346	The aim of the present study was to fabricate and characterise chitosan scaffolds from animal and fungal sources, with or without gelatine as a co-polymer, and cross-linked to 3-glycidyloxyproply trimethoxysilane (GPTMS) or genipin for application in dental root tissue engineering.
33948929	12	85	theme	fungal	1656:1661	arg1	fCS-GEL-GPTMS					1683:1695	fCS-GEL-GPTMS	1683:1695	fCS-GEL-GPTMS	1683:1695	Animal chitosan-gelatine co-polymerisation increased scaffold compressive strength, while fungal chitosan scaffolds (fCS-GEL-GPTMS) had the fastest degradation rate, losing 80 % of their weight by day 21.
33948929	12	85	theme	fungal	1656:1661	arg1	scaffolds					1672:1680	fungal chitosan scaffolds	1656:1680	fungal chitosan scaffolds (fCS-GEL-GPTMS)	1656:1696	Animal chitosan-gelatine co-polymerisation increased scaffold compressive strength, while fungal chitosan scaffolds (fCS-GEL-GPTMS) had the fastest degradation rate, losing 80 % of their weight by day 21.
33948929	4	86	dep	Fourier	678:684	arg1	transform					686:694	transform	686:694	transform infrared-attenuated total reflectance spectroscopy	686:745	Chemical composition and cross-linking were evaluated by Fourier transform infrared-attenuated total reflectance spectroscopy.
33948929	13	87	theme	enhanced	1822:1829	arg1	chitosan					1831:1838	enhanced chitosan	1822:1838	enhanced chitosan	1822:1838	Gelatine co-polymerisation and GPTMS cross-linking enhanced chitosan scaffolds bioactivity through the formation of an apatite layer as well as improved hDPSCs attachment and viability.
33948929	7	88	theme	X-ray	1003:1007	arg1	spectroscopy					1009:1020	associated electron dispersive X-ray spectroscopy	972:1020	associated electron dispersive X-ray spectroscopy	972:1020	Scaffold bioactivity immersed in simulated body fluid was evaluated by SEM, with associated electron dispersive X-ray spectroscopy, and apatite formation was examined by X-ray diffraction.
33948929	12	89	theme	compressive	1628:1638	arg1	strength					1640:1647	scaffold compressive strength	1619:1647	scaffold compressive strength	1619:1647	Animal chitosan-gelatine co-polymerisation increased scaffold compressive strength, while fungal chitosan scaffolds (fCS-GEL-GPTMS) had the fastest degradation rate, losing 80 % of their weight by day 21.
33948929	7	90	theme	electron	983:990	arg1	spectroscopy					1009:1020	associated electron dispersive X-ray spectroscopy	972:1020	associated electron dispersive X-ray spectroscopy	972:1020	Scaffold bioactivity immersed in simulated body fluid was evaluated by SEM, with associated electron dispersive X-ray spectroscopy, and apatite formation was examined by X-ray diffraction.
33396884	2	0	theme	lignicolous	463:473	arg1	basidiomycetes					475:488	lignicolous basidiomycetes	463:488	lignicolous basidiomycetes growing in nature	463:506	Materials containing commercially available kraft fibers (KF), laboratory-made highly fibrillated hemp fibers (HF) and fungal fibers (FF) obtained from fruiting bodies of lignicolous basidiomycetes growing in nature were prepared using paper production techniques and evaluated for their mechanical and air permeability properties.
33396884	2	1	theme	permeability	599:610	arg1	properties					612:621	their mechanical and air permeability properties	574:621	properties	612:621	Materials containing commercially available kraft fibers (KF), laboratory-made highly fibrillated hemp fibers (HF) and fungal fibers (FF) obtained from fruiting bodies of lignicolous basidiomycetes growing in nature were prepared using paper production techniques and evaluated for their mechanical and air permeability properties.
33396884	0	2	theme	Fungal	77:82	arg1	Hyphae					84:89	Fungal Hyphae	77:89	Fungal Hyphae	77:89	Mechanical and Air Permeability Performance of Novel Biobased Materials from Fungal Hyphae and Cellulose Fibers.
33396884	2	3	theme	air	595:597	arg1	properties					612:621	their mechanical and air permeability properties	574:621	properties	612:621	Materials containing commercially available kraft fibers (KF), laboratory-made highly fibrillated hemp fibers (HF) and fungal fibers (FF) obtained from fruiting bodies of lignicolous basidiomycetes growing in nature were prepared using paper production techniques and evaluated for their mechanical and air permeability properties.
33396884	1	4	theme	cellulose	161:169	arg1	fibers					171:176	cellulose fibers	161:176	cellulose fibers	161:176	Novel biobased materials from fungal hyphae and cellulose fibers have been proposed to address the increasing demand for natural materials in personal protective equipment (PPE).
33396884	1	5	theme	personal	255:262	arg1	PPE					286:288	PPE	286:288	PPE	286:288	Novel biobased materials from fungal hyphae and cellulose fibers have been proposed to address the increasing demand for natural materials in personal protective equipment (PPE).
33396884	1	5	theme	personal	255:262	arg1	equipment					275:283	personal protective equipment	255:283	personal protective equipment (PPE)	255:289	Novel biobased materials from fungal hyphae and cellulose fibers have been proposed to address the increasing demand for natural materials in personal protective equipment (PPE).
33396884	0	6	theme	Cellulose	95:103	arg1	Fibers					105:110	Cellulose Fibers	95:110	Cellulose Fibers	95:110	Mechanical and Air Permeability Performance of Novel Biobased Materials from Fungal Hyphae and Cellulose Fibers.
33396884	4	7	theme	air	753:755	arg1	permeability					757:768	air permeability	753:768	air permeability	753:768	The tensile index of materials was in the range of 8-60 Nm/g and air permeability ranged from 32-23,990 mL/min, depending on the composition of materials.
33396884	7	8	theme	fungal	1370:1375	arg1	hyphae					1377:1382	fungal hyphae	1370:1382	fungal hyphae	1370:1382	Conclusions were drawn about the appropriateness of fungal hyphae for use in papermaking-like technologies together with cellulose fibers.
33396884	1	9	theme	protective	264:273	arg1	PPE					286:288	PPE	286:288	PPE	286:288	Novel biobased materials from fungal hyphae and cellulose fibers have been proposed to address the increasing demand for natural materials in personal protective equipment (PPE).
33396884	1	9	theme	protective	264:273	arg1	equipment					275:283	personal protective equipment	255:283	personal protective equipment (PPE)	255:289	Novel biobased materials from fungal hyphae and cellulose fibers have been proposed to address the increasing demand for natural materials in personal protective equipment (PPE).
33396884	2	10	dep	fibers	342:347	arg1	fibers					395:400	highly fibrillated hemp fibers	371:400	highly fibrillated hemp fibers (HF)	371:405	Materials containing commercially available kraft fibers (KF), laboratory-made highly fibrillated hemp fibers (HF) and fungal fibers (FF) obtained from fruiting bodies of lignicolous basidiomycetes growing in nature were prepared using paper production techniques and evaluated for their mechanical and air permeability properties.
33396884	2	10	dep	fibers	342:347	arg1	FF					426:427	FF	426:427	FF	426:427	Materials containing commercially available kraft fibers (KF), laboratory-made highly fibrillated hemp fibers (HF) and fungal fibers (FF) obtained from fruiting bodies of lignicolous basidiomycetes growing in nature were prepared using paper production techniques and evaluated for their mechanical and air permeability properties.
33396884	2	10	dep	fibers	342:347	arg1	fibers					418:423	fungal fibers	411:423	fungal fibers (FF)	411:428	Materials containing commercially available kraft fibers (KF), laboratory-made highly fibrillated hemp fibers (HF) and fungal fibers (FF) obtained from fruiting bodies of lignicolous basidiomycetes growing in nature were prepared using paper production techniques and evaluated for their mechanical and air permeability properties.
33396884	2	10	dep	fibers	342:347	arg1	HF					403:404	HF	403:404	HF	403:404	Materials containing commercially available kraft fibers (KF), laboratory-made highly fibrillated hemp fibers (HF) and fungal fibers (FF) obtained from fruiting bodies of lignicolous basidiomycetes growing in nature were prepared using paper production techniques and evaluated for their mechanical and air permeability properties.
33396884	7	11	theme	cellulose	1439:1447	arg1	fibers					1449:1454	cellulose fibers	1439:1454	cellulose fibers	1439:1454	Conclusions were drawn about the appropriateness of fungal hyphae for use in papermaking-like technologies together with cellulose fibers.
33396884	5	12	theme	higher	938:943	arg1	permeability					949:960	higher air permeability	938:960	higher air permeability	938:960	HF was the key component for strength; however, the addition of FF to compositions resulted in higher air permeability.
33396884	6	13	from	composting	1243:1252	arg1	substrate					1257:1265	substrate	1257:1265	substrate	1257:1265	Chemical composition analysis (Fourier-transform infrared spectroscopy) revealed the presence of natural polysaccharides, mainly cellulose and chitin, as well as the appropriate elemental distribution of components C, H and N. Biodegradation potential was proven by a 30-day-long composting in substrate, which resulted in an 8-62% drop in the C/N ratio.
33396884	4	14	theme	materials	832:840	arg1	composition					817:827	the composition	813:827	the composition of materials	813:840	The tensile index of materials was in the range of 8-60 Nm/g and air permeability ranged from 32-23,990 mL/min, depending on the composition of materials.
33396884	1	15	from	hyphae	150:155	arg1	materials					128:136	Novel biobased materials	113:136	Novel biobased materials from fungal hyphae and cellulose fibers	113:176	Novel biobased materials from fungal hyphae and cellulose fibers have been proposed to address the increasing demand for natural materials in personal protective equipment (PPE).
33396884	5	16	theme	air	945:947	arg1	permeability					949:960	higher air permeability	938:960	higher air permeability	938:960	HF was the key component for strength; however, the addition of FF to compositions resulted in higher air permeability.
33396884	5	17	theme	key	854:856	arg1	HF					843:844	HF	843:844	HF	843:844	HF was the key component for strength; however, the addition of FF to compositions resulted in higher air permeability.
33396884	5	17	theme	key	854:856	arg1	component					858:866	the key component	850:866	the key component for strength	850:879	HF was the key component for strength; however, the addition of FF to compositions resulted in higher air permeability.
33396884	4	18	theme	permeability	757:768	arg1	range					730:734	the range	726:734	the range of 8-60 Nm/g and air permeability	726:768	The tensile index of materials was in the range of 8-60 Nm/g and air permeability ranged from 32-23,990 mL/min, depending on the composition of materials.
33396884	6	19	theme	polysaccharides	1068:1082	arg1	distribution					1151:1162	the appropriate elemental distribution	1125:1162	the appropriate elemental distribution of components	1125:1176	Chemical composition analysis (Fourier-transform infrared spectroscopy) revealed the presence of natural polysaccharides, mainly cellulose and chitin, as well as the appropriate elemental distribution of components C, H and N. Biodegradation potential was proven by a 30-day-long composting in substrate, which resulted in an 8-62% drop in the C/N ratio.
33396884	6	19	theme	polysaccharides	1068:1082	arg1	presence					1048:1055	the presence	1044:1055	the presence of natural polysaccharides, mainly cellulose and chitin	1044:1111	Chemical composition analysis (Fourier-transform infrared spectroscopy) revealed the presence of natural polysaccharides, mainly cellulose and chitin, as well as the appropriate elemental distribution of components C, H and N. Biodegradation potential was proven by a 30-day-long composting in substrate, which resulted in an 8-62% drop in the C/N ratio.
33396884	8	20	theme	synthetic	1516:1524	arg1	melt					1526:1529	synthetic melt	1516:1529	synthetic melt	1516:1529	Developed materials can be considered as an alternative to synthetic melt and spun-blown materials for PPE.
33396884	6	21	theme	30-day-long	1231:1241	arg1	composting					1243:1252	a 30-day-long composting	1229:1252	a 30-day-long composting	1229:1252	Chemical composition analysis (Fourier-transform infrared spectroscopy) revealed the presence of natural polysaccharides, mainly cellulose and chitin, as well as the appropriate elemental distribution of components C, H and N. Biodegradation potential was proven by a 30-day-long composting in substrate, which resulted in an 8-62% drop in the C/N ratio.
33396884	8	22	theme	spun-blown	1535:1544	arg1	materials					1546:1554	spun-blown materials	1535:1554	spun-blown materials	1535:1554	Developed materials can be considered as an alternative to synthetic melt and spun-blown materials for PPE.
33396884	2	23	theme	available	326:334	arg1	KF					350:351	KF	350:351	KF	350:351	Materials containing commercially available kraft fibers (KF), laboratory-made highly fibrillated hemp fibers (HF) and fungal fibers (FF) obtained from fruiting bodies of lignicolous basidiomycetes growing in nature were prepared using paper production techniques and evaluated for their mechanical and air permeability properties.
33396884	2	23	theme	available	326:334	arg1	laboratory-made					355:369	laboratory-made	355:369	laboratory-made	355:369	Materials containing commercially available kraft fibers (KF), laboratory-made highly fibrillated hemp fibers (HF) and fungal fibers (FF) obtained from fruiting bodies of lignicolous basidiomycetes growing in nature were prepared using paper production techniques and evaluated for their mechanical and air permeability properties.
33396884	2	23	theme	available	326:334	arg1	fibers					342:347	commercially available kraft fibers	313:347	commercially available kraft fibers (KF)	313:352	Materials containing commercially available kraft fibers (KF), laboratory-made highly fibrillated hemp fibers (HF) and fungal fibers (FF) obtained from fruiting bodies of lignicolous basidiomycetes growing in nature were prepared using paper production techniques and evaluated for their mechanical and air permeability properties.
33396884	6	24	theme	8-62	1289:1292	arg1	%					1293:1293	%	1293:1293	%	1293:1293	Chemical composition analysis (Fourier-transform infrared spectroscopy) revealed the presence of natural polysaccharides, mainly cellulose and chitin, as well as the appropriate elemental distribution of components C, H and N. Biodegradation potential was proven by a 30-day-long composting in substrate, which resulted in an 8-62% drop in the C/N ratio.
33396884	2	25	theme	hemp	390:393	arg1	fibers					395:400	highly fibrillated hemp fibers	371:400	highly fibrillated hemp fibers (HF)	371:405	Materials containing commercially available kraft fibers (KF), laboratory-made highly fibrillated hemp fibers (HF) and fungal fibers (FF) obtained from fruiting bodies of lignicolous basidiomycetes growing in nature were prepared using paper production techniques and evaluated for their mechanical and air permeability properties.
33396884	2	25	theme	hemp	390:393	arg1	HF					403:404	HF	403:404	HF	403:404	Materials containing commercially available kraft fibers (KF), laboratory-made highly fibrillated hemp fibers (HF) and fungal fibers (FF) obtained from fruiting bodies of lignicolous basidiomycetes growing in nature were prepared using paper production techniques and evaluated for their mechanical and air permeability properties.
33396884	7	26	theme	papermaking-like	1395:1410	arg1	technologies					1412:1423	papermaking-like technologies	1395:1423	papermaking-like technologies together with cellulose fibers	1395:1454	Conclusions were drawn about the appropriateness of fungal hyphae for use in papermaking-like technologies together with cellulose fibers.
33396884	6	27	theme	elemental	1141:1149	arg1	distribution					1151:1162	the appropriate elemental distribution	1125:1162	the appropriate elemental distribution of components	1125:1176	Chemical composition analysis (Fourier-transform infrared spectroscopy) revealed the presence of natural polysaccharides, mainly cellulose and chitin, as well as the appropriate elemental distribution of components C, H and N. Biodegradation potential was proven by a 30-day-long composting in substrate, which resulted in an 8-62% drop in the C/N ratio.
33396884	2	28	theme	fibrillated	378:388	arg1	fibers					395:400	highly fibrillated hemp fibers	371:400	highly fibrillated hemp fibers (HF)	371:405	Materials containing commercially available kraft fibers (KF), laboratory-made highly fibrillated hemp fibers (HF) and fungal fibers (FF) obtained from fruiting bodies of lignicolous basidiomycetes growing in nature were prepared using paper production techniques and evaluated for their mechanical and air permeability properties.
33396884	2	28	theme	fibrillated	378:388	arg1	HF					403:404	HF	403:404	HF	403:404	Materials containing commercially available kraft fibers (KF), laboratory-made highly fibrillated hemp fibers (HF) and fungal fibers (FF) obtained from fruiting bodies of lignicolous basidiomycetes growing in nature were prepared using paper production techniques and evaluated for their mechanical and air permeability properties.
33396884	0	29	theme	Permeability	19:30	arg1	Performance					32:42	Air Permeability Performance	15:42	Air Permeability Performance of Novel Biobased Materials from Fungal Hyphae and Cellulose Fibers	15:110	Mechanical and Air Permeability Performance of Novel Biobased Materials from Fungal Hyphae and Cellulose Fibers.
33396884	6	30	from	drop	1295:1298	arg1	ratio					1311:1315	the C/N ratio	1303:1315	the C/N ratio	1303:1315	Chemical composition analysis (Fourier-transform infrared spectroscopy) revealed the presence of natural polysaccharides, mainly cellulose and chitin, as well as the appropriate elemental distribution of components C, H and N. Biodegradation potential was proven by a 30-day-long composting in substrate, which resulted in an 8-62% drop in the C/N ratio.
33396884	6	31	theme	C/N	1307:1309	arg1	ratio					1311:1315	the C/N ratio	1303:1315	the C/N ratio	1303:1315	Chemical composition analysis (Fourier-transform infrared spectroscopy) revealed the presence of natural polysaccharides, mainly cellulose and chitin, as well as the appropriate elemental distribution of components C, H and N. Biodegradation potential was proven by a 30-day-long composting in substrate, which resulted in an 8-62% drop in the C/N ratio.
33396884	4	32	theme	Nm/g	744:747	arg1	range					730:734	the range	726:734	the range of 8-60 Nm/g and air permeability	726:768	The tensile index of materials was in the range of 8-60 Nm/g and air permeability ranged from 32-23,990 mL/min, depending on the composition of materials.
33396884	2	33	theme	fungal	411:416	arg1	FF					426:427	FF	426:427	FF	426:427	Materials containing commercially available kraft fibers (KF), laboratory-made highly fibrillated hemp fibers (HF) and fungal fibers (FF) obtained from fruiting bodies of lignicolous basidiomycetes growing in nature were prepared using paper production techniques and evaluated for their mechanical and air permeability properties.
33396884	2	33	theme	fungal	411:416	arg1	fibers					418:423	fungal fibers	411:423	fungal fibers (FF)	411:428	Materials containing commercially available kraft fibers (KF), laboratory-made highly fibrillated hemp fibers (HF) and fungal fibers (FF) obtained from fruiting bodies of lignicolous basidiomycetes growing in nature were prepared using paper production techniques and evaluated for their mechanical and air permeability properties.
33396884	0	34	theme	Air	15:17	arg1	Performance					32:42	Air Permeability Performance	15:42	Air Permeability Performance of Novel Biobased Materials from Fungal Hyphae and Cellulose Fibers	15:110	Mechanical and Air Permeability Performance of Novel Biobased Materials from Fungal Hyphae and Cellulose Fibers.
33396884	2	35	theme	production	534:543	arg1	techniques					545:554	paper production techniques	528:554	paper production techniques	528:554	Materials containing commercially available kraft fibers (KF), laboratory-made highly fibrillated hemp fibers (HF) and fungal fibers (FF) obtained from fruiting bodies of lignicolous basidiomycetes growing in nature were prepared using paper production techniques and evaluated for their mechanical and air permeability properties.
33396884	0	36	from	Hyphae	84:89	arg1	Performance					32:42	Air Permeability Performance	15:42	Air Permeability Performance of Novel Biobased Materials from Fungal Hyphae and Cellulose Fibers	15:110	Mechanical and Air Permeability Performance of Novel Biobased Materials from Fungal Hyphae and Cellulose Fibers.
33396884	4	37	theme	8-60	739:742	arg1	Nm/g					744:747	8-60 Nm/g	739:747	8-60 Nm/g	739:747	The tensile index of materials was in the range of 8-60 Nm/g and air permeability ranged from 32-23,990 mL/min, depending on the composition of materials.
33396884	7	38	theme	hyphae	1377:1382	arg1	appropriateness					1351:1365	the appropriateness	1347:1365	the appropriateness of fungal hyphae for use in papermaking-like technologies together with cellulose fibers	1347:1454	Conclusions were drawn about the appropriateness of fungal hyphae for use in papermaking-like technologies together with cellulose fibers.
33396884	4	39	theme	tensile	692:698	arg1	index					700:704	The tensile index	688:704	The tensile index of materials was in the range of 8-60 Nm/g and air permeability	688:768	The tensile index of materials was in the range of 8-60 Nm/g and air permeability ranged from 32-23,990 mL/min, depending on the composition of materials.
33396884	6	40	theme	natural	1060:1066	arg1	chitin					1106:1111	chitin	1106:1111	chitin	1106:1111	Chemical composition analysis (Fourier-transform infrared spectroscopy) revealed the presence of natural polysaccharides, mainly cellulose and chitin, as well as the appropriate elemental distribution of components C, H and N. Biodegradation potential was proven by a 30-day-long composting in substrate, which resulted in an 8-62% drop in the C/N ratio.
33396884	6	40	theme	natural	1060:1066	arg1	polysaccharides					1068:1082	natural polysaccharides	1060:1082	natural polysaccharides	1060:1082	Chemical composition analysis (Fourier-transform infrared spectroscopy) revealed the presence of natural polysaccharides, mainly cellulose and chitin, as well as the appropriate elemental distribution of components C, H and N. Biodegradation potential was proven by a 30-day-long composting in substrate, which resulted in an 8-62% drop in the C/N ratio.
33396884	6	40	theme	natural	1060:1066	arg1	cellulose					1092:1100	cellulose	1092:1100	cellulose	1092:1100	Chemical composition analysis (Fourier-transform infrared spectroscopy) revealed the presence of natural polysaccharides, mainly cellulose and chitin, as well as the appropriate elemental distribution of components C, H and N. Biodegradation potential was proven by a 30-day-long composting in substrate, which resulted in an 8-62% drop in the C/N ratio.
33396884	4	41	theme	materials	709:717	arg1	index					700:704	The tensile index	688:704	The tensile index of materials was in the range of 8-60 Nm/g and air permeability	688:768	The tensile index of materials was in the range of 8-60 Nm/g and air permeability ranged from 32-23,990 mL/min, depending on the composition of materials.
33396884	2	42	theme	paper	528:532	arg1	techniques					545:554	paper production techniques	528:554	paper production techniques	528:554	Materials containing commercially available kraft fibers (KF), laboratory-made highly fibrillated hemp fibers (HF) and fungal fibers (FF) obtained from fruiting bodies of lignicolous basidiomycetes growing in nature were prepared using paper production techniques and evaluated for their mechanical and air permeability properties.
33396884	3	43	theme	network	656:662	arg1	structure					664:672	the network structure	652:672	the network structure of materials	652:685	SEM and microscopy revealed the network structure of materials.
33396884	2	44	theme	fruiting	444:451	arg1	bodies					453:458	fruiting bodies	444:458	fruiting bodies of lignicolous basidiomycetes growing in nature	444:506	Materials containing commercially available kraft fibers (KF), laboratory-made highly fibrillated hemp fibers (HF) and fungal fibers (FF) obtained from fruiting bodies of lignicolous basidiomycetes growing in nature were prepared using paper production techniques and evaluated for their mechanical and air permeability properties.
33396884	6	45	theme	composition	972:982	arg1	analysis					984:991	Chemical composition analysis	963:991	Chemical composition analysis (Fourier-transform infrared spectroscopy)	963:1033	Chemical composition analysis (Fourier-transform infrared spectroscopy) revealed the presence of natural polysaccharides, mainly cellulose and chitin, as well as the appropriate elemental distribution of components C, H and N. Biodegradation potential was proven by a 30-day-long composting in substrate, which resulted in an 8-62% drop in the C/N ratio.
33396884	6	45	theme	composition	972:982	arg1	spectroscopy					1021:1032	Fourier-transform infrared spectroscopy	994:1032	Fourier-transform infrared spectroscopy	994:1032	Chemical composition analysis (Fourier-transform infrared spectroscopy) revealed the presence of natural polysaccharides, mainly cellulose and chitin, as well as the appropriate elemental distribution of components C, H and N. Biodegradation potential was proven by a 30-day-long composting in substrate, which resulted in an 8-62% drop in the C/N ratio.
33396884	6	46	theme	Fourier-transform	994:1010	arg1	analysis					984:991	Chemical composition analysis	963:991	Chemical composition analysis (Fourier-transform infrared spectroscopy)	963:1033	Chemical composition analysis (Fourier-transform infrared spectroscopy) revealed the presence of natural polysaccharides, mainly cellulose and chitin, as well as the appropriate elemental distribution of components C, H and N. Biodegradation potential was proven by a 30-day-long composting in substrate, which resulted in an 8-62% drop in the C/N ratio.
33396884	6	46	theme	Fourier-transform	994:1010	arg1	spectroscopy					1021:1032	Fourier-transform infrared spectroscopy	994:1032	Fourier-transform infrared spectroscopy	994:1032	Chemical composition analysis (Fourier-transform infrared spectroscopy) revealed the presence of natural polysaccharides, mainly cellulose and chitin, as well as the appropriate elemental distribution of components C, H and N. Biodegradation potential was proven by a 30-day-long composting in substrate, which resulted in an 8-62% drop in the C/N ratio.
33396884	1	47	theme	increasing	212:221	arg1	demand					223:228	the increasing demand	208:228	the increasing demand for natural materials in personal protective equipment (PPE)	208:289	Novel biobased materials from fungal hyphae and cellulose fibers have been proposed to address the increasing demand for natural materials in personal protective equipment (PPE).
33396884	0	48	theme	Biobased	53:60	arg1	Materials					62:70	Novel Biobased Materials	47:70	Novel Biobased Materials	47:70	Mechanical and Air Permeability Performance of Novel Biobased Materials from Fungal Hyphae and Cellulose Fibers.
33396884	2	49	contain	containing	302:311	arg2	laboratory-made					355:369	laboratory-made	355:369	laboratory-made	355:369	Materials containing commercially available kraft fibers (KF), laboratory-made highly fibrillated hemp fibers (HF) and fungal fibers (FF) obtained from fruiting bodies of lignicolous basidiomycetes growing in nature were prepared using paper production techniques and evaluated for their mechanical and air permeability properties.
33396884	2	49	contain	containing	302:311	arg2	KF					350:351	KF	350:351	KF	350:351	Materials containing commercially available kraft fibers (KF), laboratory-made highly fibrillated hemp fibers (HF) and fungal fibers (FF) obtained from fruiting bodies of lignicolous basidiomycetes growing in nature were prepared using paper production techniques and evaluated for their mechanical and air permeability properties.
33396884	2	49	contain	containing	302:311	arg2	fibers					342:347	commercially available kraft fibers	313:347	commercially available kraft fibers (KF)	313:352	Materials containing commercially available kraft fibers (KF), laboratory-made highly fibrillated hemp fibers (HF) and fungal fibers (FF) obtained from fruiting bodies of lignicolous basidiomycetes growing in nature were prepared using paper production techniques and evaluated for their mechanical and air permeability properties.
33396884	2	49	contain	containing	302:311	arg1	Materials					292:300	Materials	292:300	Materials containing commercially available kraft fibers (KF), laboratory-made highly fibrillated hemp fibers (HF) and fungal fibers (FF) obtained from fruiting bodies of lignicolous basidiomycetes growing in nature	292:506	Materials containing commercially available kraft fibers (KF), laboratory-made highly fibrillated hemp fibers (HF) and fungal fibers (FF) obtained from fruiting bodies of lignicolous basidiomycetes growing in nature were prepared using paper production techniques and evaluated for their mechanical and air permeability properties.
33396884	6	50	theme	%	1293:1293	arg1	drop					1295:1298	an 8-62% drop	1286:1298	an 8-62% drop in the C/N ratio	1286:1315	Chemical composition analysis (Fourier-transform infrared spectroscopy) revealed the presence of natural polysaccharides, mainly cellulose and chitin, as well as the appropriate elemental distribution of components C, H and N. Biodegradation potential was proven by a 30-day-long composting in substrate, which resulted in an 8-62% drop in the C/N ratio.
33396884	2	51	theme	basidiomycetes	475:488	arg1	bodies					453:458	fruiting bodies	444:458	fruiting bodies of lignicolous basidiomycetes growing in nature	444:506	Materials containing commercially available kraft fibers (KF), laboratory-made highly fibrillated hemp fibers (HF) and fungal fibers (FF) obtained from fruiting bodies of lignicolous basidiomycetes growing in nature were prepared using paper production techniques and evaluated for their mechanical and air permeability properties.
33396884	1	52	theme	Novel	113:117	arg1	materials					128:136	Novel biobased materials	113:136	Novel biobased materials from fungal hyphae and cellulose fibers	113:176	Novel biobased materials from fungal hyphae and cellulose fibers have been proposed to address the increasing demand for natural materials in personal protective equipment (PPE).
33396884	0	53	theme	Novel	47:51	arg1	Materials					62:70	Novel Biobased Materials	47:70	Novel Biobased Materials	47:70	Mechanical and Air Permeability Performance of Novel Biobased Materials from Fungal Hyphae and Cellulose Fibers.
33396884	8	54	theme	Developed	1457:1465	arg1	materials					1467:1475	Developed materials	1457:1475	Developed materials	1457:1475	Developed materials can be considered as an alternative to synthetic melt and spun-blown materials for PPE.
33396884	8	54	theme	Developed	1457:1465	arg1	alternative					1501:1511	an alternative	1498:1511	an alternative to synthetic melt and spun-blown materials for PPE	1498:1562	Developed materials can be considered as an alternative to synthetic melt and spun-blown materials for PPE.
33396884	1	55	from	materials	242:250	arg1	PPE					286:288	PPE	286:288	PPE	286:288	Novel biobased materials from fungal hyphae and cellulose fibers have been proposed to address the increasing demand for natural materials in personal protective equipment (PPE).
33396884	1	55	from	materials	242:250	arg1	equipment					275:283	personal protective equipment	255:283	personal protective equipment (PPE)	255:289	Novel biobased materials from fungal hyphae and cellulose fibers have been proposed to address the increasing demand for natural materials in personal protective equipment (PPE).
33396884	3	56	theme	materials	677:685	arg1	structure					664:672	the network structure	652:672	the network structure of materials	652:685	SEM and microscopy revealed the network structure of materials.
33396884	1	57	theme	biobased	119:126	arg1	materials					128:136	Novel biobased materials	113:136	Novel biobased materials from fungal hyphae and cellulose fibers	113:176	Novel biobased materials from fungal hyphae and cellulose fibers have been proposed to address the increasing demand for natural materials in personal protective equipment (PPE).
33396884	5	58	theme	FF	907:908	arg1	addition					895:902	the addition	891:902	the addition of FF to compositions	891:924	HF was the key component for strength; however, the addition of FF to compositions resulted in higher air permeability.
33396884	0	59	from	Fibers	105:110	arg1	Performance					32:42	Air Permeability Performance	15:42	Air Permeability Performance of Novel Biobased Materials from Fungal Hyphae and Cellulose Fibers	15:110	Mechanical and Air Permeability Performance of Novel Biobased Materials from Fungal Hyphae and Cellulose Fibers.
33396884	6	60	theme	Biodegradation	1190:1203	arg1	potential					1205:1213	N. Biodegradation potential	1187:1213	N. Biodegradation potential	1187:1213	Chemical composition analysis (Fourier-transform infrared spectroscopy) revealed the presence of natural polysaccharides, mainly cellulose and chitin, as well as the appropriate elemental distribution of components C, H and N. Biodegradation potential was proven by a 30-day-long composting in substrate, which resulted in an 8-62% drop in the C/N ratio.
33396884	6	61	theme	appropriate	1129:1139	arg1	distribution					1151:1162	the appropriate elemental distribution	1125:1162	the appropriate elemental distribution of components	1125:1176	Chemical composition analysis (Fourier-transform infrared spectroscopy) revealed the presence of natural polysaccharides, mainly cellulose and chitin, as well as the appropriate elemental distribution of components C, H and N. Biodegradation potential was proven by a 30-day-long composting in substrate, which resulted in an 8-62% drop in the C/N ratio.
33396884	6	62	theme	Chemical	963:970	arg1	analysis					984:991	Chemical composition analysis	963:991	Chemical composition analysis (Fourier-transform infrared spectroscopy)	963:1033	Chemical composition analysis (Fourier-transform infrared spectroscopy) revealed the presence of natural polysaccharides, mainly cellulose and chitin, as well as the appropriate elemental distribution of components C, H and N. Biodegradation potential was proven by a 30-day-long composting in substrate, which resulted in an 8-62% drop in the C/N ratio.
33396884	6	62	theme	Chemical	963:970	arg1	spectroscopy					1021:1032	Fourier-transform infrared spectroscopy	994:1032	Fourier-transform infrared spectroscopy	994:1032	Chemical composition analysis (Fourier-transform infrared spectroscopy) revealed the presence of natural polysaccharides, mainly cellulose and chitin, as well as the appropriate elemental distribution of components C, H and N. Biodegradation potential was proven by a 30-day-long composting in substrate, which resulted in an 8-62% drop in the C/N ratio.
33396884	6	63	theme	N.	1187:1188	arg1	potential					1205:1213	N. Biodegradation potential	1187:1213	N. Biodegradation potential	1187:1213	Chemical composition analysis (Fourier-transform infrared spectroscopy) revealed the presence of natural polysaccharides, mainly cellulose and chitin, as well as the appropriate elemental distribution of components C, H and N. Biodegradation potential was proven by a 30-day-long composting in substrate, which resulted in an 8-62% drop in the C/N ratio.
33396884	2	64	theme	kraft	336:340	arg1	KF					350:351	KF	350:351	KF	350:351	Materials containing commercially available kraft fibers (KF), laboratory-made highly fibrillated hemp fibers (HF) and fungal fibers (FF) obtained from fruiting bodies of lignicolous basidiomycetes growing in nature were prepared using paper production techniques and evaluated for their mechanical and air permeability properties.
33396884	2	64	theme	kraft	336:340	arg1	laboratory-made					355:369	laboratory-made	355:369	laboratory-made	355:369	Materials containing commercially available kraft fibers (KF), laboratory-made highly fibrillated hemp fibers (HF) and fungal fibers (FF) obtained from fruiting bodies of lignicolous basidiomycetes growing in nature were prepared using paper production techniques and evaluated for their mechanical and air permeability properties.
33396884	2	64	theme	kraft	336:340	arg1	fibers					342:347	commercially available kraft fibers	313:347	commercially available kraft fibers (KF)	313:352	Materials containing commercially available kraft fibers (KF), laboratory-made highly fibrillated hemp fibers (HF) and fungal fibers (FF) obtained from fruiting bodies of lignicolous basidiomycetes growing in nature were prepared using paper production techniques and evaluated for their mechanical and air permeability properties.
33396884	6	65	theme	infrared	1012:1019	arg1	analysis					984:991	Chemical composition analysis	963:991	Chemical composition analysis (Fourier-transform infrared spectroscopy)	963:1033	Chemical composition analysis (Fourier-transform infrared spectroscopy) revealed the presence of natural polysaccharides, mainly cellulose and chitin, as well as the appropriate elemental distribution of components C, H and N. Biodegradation potential was proven by a 30-day-long composting in substrate, which resulted in an 8-62% drop in the C/N ratio.
33396884	6	65	theme	infrared	1012:1019	arg1	spectroscopy					1021:1032	Fourier-transform infrared spectroscopy	994:1032	Fourier-transform infrared spectroscopy	994:1032	Chemical composition analysis (Fourier-transform infrared spectroscopy) revealed the presence of natural polysaccharides, mainly cellulose and chitin, as well as the appropriate elemental distribution of components C, H and N. Biodegradation potential was proven by a 30-day-long composting in substrate, which resulted in an 8-62% drop in the C/N ratio.
33396884	1	66	theme	fungal	143:148	arg1	hyphae					150:155	fungal hyphae	143:155	fungal hyphae	143:155	Novel biobased materials from fungal hyphae and cellulose fibers have been proposed to address the increasing demand for natural materials in personal protective equipment (PPE).
33396884	6	67	theme	components	1167:1176	arg1	distribution					1151:1162	the appropriate elemental distribution	1125:1162	the appropriate elemental distribution of components	1125:1176	Chemical composition analysis (Fourier-transform infrared spectroscopy) revealed the presence of natural polysaccharides, mainly cellulose and chitin, as well as the appropriate elemental distribution of components C, H and N. Biodegradation potential was proven by a 30-day-long composting in substrate, which resulted in an 8-62% drop in the C/N ratio.
33396884	6	67	theme	components	1167:1176	arg1	presence					1048:1055	the presence	1044:1055	the presence of natural polysaccharides, mainly cellulose and chitin	1044:1111	Chemical composition analysis (Fourier-transform infrared spectroscopy) revealed the presence of natural polysaccharides, mainly cellulose and chitin, as well as the appropriate elemental distribution of components C, H and N. Biodegradation potential was proven by a 30-day-long composting in substrate, which resulted in an 8-62% drop in the C/N ratio.
33396884	1	68	theme	natural	234:240	arg1	materials					242:250	natural materials	234:250	natural materials in personal protective equipment (PPE)	234:289	Novel biobased materials from fungal hyphae and cellulose fibers have been proposed to address the increasing demand for natural materials in personal protective equipment (PPE).
33396884	0	69	theme	Materials	62:70	arg1	Performance					32:42	Air Permeability Performance	15:42	Air Permeability Performance of Novel Biobased Materials from Fungal Hyphae and Cellulose Fibers	15:110	Mechanical and Air Permeability Performance of Novel Biobased Materials from Fungal Hyphae and Cellulose Fibers.
33396884	1	70	from	fibers	171:176	arg1	materials					128:136	Novel biobased materials	113:136	Novel biobased materials from fungal hyphae and cellulose fibers	113:176	Novel biobased materials from fungal hyphae and cellulose fibers have been proposed to address the increasing demand for natural materials in personal protective equipment (PPE).
33396884	7	71	from	use	1388:1390	arg1	technologies					1412:1423	papermaking-like technologies	1395:1423	papermaking-like technologies together with cellulose fibers	1395:1454	Conclusions were drawn about the appropriateness of fungal hyphae for use in papermaking-like technologies together with cellulose fibers.
32105835	2	0	theme	pathological	605:616	arg1	SF					618:619	pathological SF	605:619	pathological SF	605:619	The objectives of this study were to (i) evaluate the friction coefficients and induced shear strains in articular cartilage when lubricated with pathological SF, (ii) identify the effect of hyaluronic acid (HA) supplementation on friction coefficients and shear strains, and (iii) identify SF biomarkers that correlate with lubricating function.
32105835	9	1	theme	SF	1931:1932	arg1	behaviors					1946:1954	SF lubricating behaviors	1931:1954	SF lubricating behaviors	1931:1954	CONCLUSION These results suggest that pathological SF is characterized by distinct tribological endotypes where SF lubricating behaviors are differentially modified by viscosupplementation and are identifiable by biomarkers.
32105835	6	2	theme	Friction	1253:1260	arg1	coefficients					1262:1273	RESULTS Friction coefficients	1245:1273	RESULTS Friction coefficients	1245:1273	RESULTS Friction coefficients were not significantly different between the inflammatory and non-inflammatory pathologies (p = 0.09), and were poorly correlated with peak tissue strains at the cartilage articular surface (R2 = 0.34).
32105835	8	3	theme	polymorphonuclear	1718:1734	arg1	neutrophil					1736:1745	polymorphonuclear neutrophil	1718:1745	polymorphonuclear neutrophil (PMN)	1718:1751	Across all pathologies there were clear relationships between polymorphonuclear neutrophil (PMN), IL-8, and lubricin concentrations with cartilage tissue strains.
32105835	8	3	theme	polymorphonuclear	1718:1734	arg1	PMN					1748:1750	PMN	1748:1750	PMN	1748:1750	Across all pathologies there were clear relationships between polymorphonuclear neutrophil (PMN), IL-8, and lubricin concentrations with cartilage tissue strains.
32105835	5	4	theme	1:1	1215:1217	arg1	ratio					1219:1223	a 1:1 ratio	1213:1223	a 1:1 ratio with HA (Hymovis®)	1213:1242	Friction coefficients and local tissue shear strain measurements were coupled using new, microscale rheological analyses by lubricating neonatal bovine cartilage explants with SF alone and in a 1:1 ratio with HA (Hymovis®).
32105835	8	5	theme	tissue	1803:1808	arg1	strains					1810:1816	cartilage tissue strains	1793:1816	cartilage tissue strains	1793:1816	Across all pathologies there were clear relationships between polymorphonuclear neutrophil (PMN), IL-8, and lubricin concentrations with cartilage tissue strains.
32105835	0	6	theme	Distinct	0:7	arg1	endotypes					22:30	Distinct tribological endotypes	0:30	Distinct tribological endotypes of pathological human synovial fluid	0:67	Distinct tribological endotypes of pathological human synovial fluid reveal characteristic biomarkers and variation in efficacy of viscosupplementation at reducing local strains in articular cartilage.
32105835	1	7	theme	OBJECTIVE	202:210	arg1	Viscosupplementation					212:231	OBJECTIVE Viscosupplementation	202:231	OBJECTIVE Viscosupplementation	202:231	OBJECTIVE Viscosupplementation has been used for decades to treat mild to moderate osteoarthritis, yet it is unknown if the lubricating function of different pathological synovial fluids (SF) vary, or if they respond differentially to viscosupplementation.
32105835	2	8	dep	identify	627:634	arg1	ii					623:624	ii	623:624	ii	623:624	The objectives of this study were to (i) evaluate the friction coefficients and induced shear strains in articular cartilage when lubricated with pathological SF, (ii) identify the effect of hyaluronic acid (HA) supplementation on friction coefficients and shear strains, and (iii) identify SF biomarkers that correlate with lubricating function.
32105835	6	9	theme	non-inflammatory	1337:1352	arg1	p = 0.09					1367:1374	p = 0.09	1367:1374	p = 0.09	1367:1374	RESULTS Friction coefficients were not significantly different between the inflammatory and non-inflammatory pathologies (p = 0.09), and were poorly correlated with peak tissue strains at the cartilage articular surface (R2 = 0.34).
32105835	6	9	theme	non-inflammatory	1337:1352	arg1	pathologies					1354:1364	the inflammatory and non-inflammatory pathologies	1316:1364	pathologies	1354:1364	RESULTS Friction coefficients were not significantly different between the inflammatory and non-inflammatory pathologies (p = 0.09), and were poorly correlated with peak tissue strains at the cartilage articular surface (R2 = 0.34).
32105835	5	10	theme	shear	1060:1064	arg1	measurements					1073:1084	local tissue shear strain measurements	1047:1084	local tissue shear strain measurements	1047:1084	Friction coefficients and local tissue shear strain measurements were coupled using new, microscale rheological analyses by lubricating neonatal bovine cartilage explants with SF alone and in a 1:1 ratio with HA (Hymovis®).
32105835	8	11	theme	IL-8	1754:1757	arg1	concentrations					1773:1786	polymorphonuclear neutrophil (PMN), IL-8, and lubricin concentrations	1718:1786	polymorphonuclear neutrophil (PMN), IL-8, and lubricin concentrations with cartilage tissue strains	1718:1816	Across all pathologies there were clear relationships between polymorphonuclear neutrophil (PMN), IL-8, and lubricin concentrations with cartilage tissue strains.
32105835	2	12	theme	shear	547:551	arg1	strains					553:559	induced shear strains	539:559	induced shear strains	539:559	The objectives of this study were to (i) evaluate the friction coefficients and induced shear strains in articular cartilage when lubricated with pathological SF, (ii) identify the effect of hyaluronic acid (HA) supplementation on friction coefficients and shear strains, and (iii) identify SF biomarkers that correlate with lubricating function.
32105835	0	13	from	biomarkers	91:100	arg1	efficacy					119:126	efficacy	119:126	efficacy of viscosupplementation	119:150	Distinct tribological endotypes of pathological human synovial fluid reveal characteristic biomarkers and variation in efficacy of viscosupplementation at reducing local strains in articular cartilage.
32105835	6	14	theme	inflammatory	1320:1331	arg1	p = 0.09					1367:1374	p = 0.09	1367:1374	p = 0.09	1367:1374	RESULTS Friction coefficients were not significantly different between the inflammatory and non-inflammatory pathologies (p = 0.09), and were poorly correlated with peak tissue strains at the cartilage articular surface (R2 = 0.34).
32105835	6	14	theme	inflammatory	1320:1331	arg1	pathologies					1354:1364	the inflammatory and non-inflammatory pathologies	1316:1364	pathologies	1354:1364	RESULTS Friction coefficients were not significantly different between the inflammatory and non-inflammatory pathologies (p = 0.09), and were poorly correlated with peak tissue strains at the cartilage articular surface (R2 = 0.34).
32105835	1	15	used	used	242:245	arg2	Viscosupplementation					212:231	OBJECTIVE Viscosupplementation	202:231	OBJECTIVE Viscosupplementation	202:231	OBJECTIVE Viscosupplementation has been used for decades to treat mild to moderate osteoarthritis, yet it is unknown if the lubricating function of different pathological synovial fluids (SF) vary, or if they respond differentially to viscosupplementation.
32105835	2	16	theme	friction	690:697	arg1	coefficients					699:710	friction coefficients	690:710	friction coefficients	690:710	The objectives of this study were to (i) evaluate the friction coefficients and induced shear strains in articular cartilage when lubricated with pathological SF, (ii) identify the effect of hyaluronic acid (HA) supplementation on friction coefficients and shear strains, and (iii) identify SF biomarkers that correlate with lubricating function.
32105835	2	17	from	effect	640:645	arg1	coefficients					699:710	friction coefficients	690:710	friction coefficients	690:710	The objectives of this study were to (i) evaluate the friction coefficients and induced shear strains in articular cartilage when lubricated with pathological SF, (ii) identify the effect of hyaluronic acid (HA) supplementation on friction coefficients and shear strains, and (iii) identify SF biomarkers that correlate with lubricating function.
32105835	2	17	from	effect	640:645	arg1	strains					722:728	shear strains	716:728	shear strains	716:728	The objectives of this study were to (i) evaluate the friction coefficients and induced shear strains in articular cartilage when lubricated with pathological SF, (ii) identify the effect of hyaluronic acid (HA) supplementation on friction coefficients and shear strains, and (iii) identify SF biomarkers that correlate with lubricating function.
32105835	2	18	theme	supplementation	671:685	arg1	effect					640:645	the effect	636:645	the effect of hyaluronic acid (HA) supplementation on friction coefficients and shear strains	636:728	The objectives of this study were to (i) evaluate the friction coefficients and induced shear strains in articular cartilage when lubricated with pathological SF, (ii) identify the effect of hyaluronic acid (HA) supplementation on friction coefficients and shear strains, and (iii) identify SF biomarkers that correlate with lubricating function.
32105835	0	19	theme	articular	181:189	arg1	cartilage					191:199	articular cartilage	181:199	articular cartilage	181:199	Distinct tribological endotypes of pathological human synovial fluid reveal characteristic biomarkers and variation in efficacy of viscosupplementation at reducing local strains in articular cartilage.
32105835	5	20	theme	local	1047:1051	arg1	measurements					1073:1084	local tissue shear strain measurements	1047:1084	local tissue shear strain measurements	1047:1084	Friction coefficients and local tissue shear strain measurements were coupled using new, microscale rheological analyses by lubricating neonatal bovine cartilage explants with SF alone and in a 1:1 ratio with HA (Hymovis®).
32105835	8	21	theme	cartilage	1793:1801	arg1	strains					1810:1816	cartilage tissue strains	1793:1816	cartilage tissue strains	1793:1816	Across all pathologies there were clear relationships between polymorphonuclear neutrophil (PMN), IL-8, and lubricin concentrations with cartilage tissue strains.
32105835	0	22	from	variation	106:114	arg1	efficacy					119:126	efficacy	119:126	efficacy of viscosupplementation	119:150	Distinct tribological endotypes of pathological human synovial fluid reveal characteristic biomarkers and variation in efficacy of viscosupplementation at reducing local strains in articular cartilage.
32105835	1	23	theme	different	350:358	arg1	SF					390:391	SF	390:391	SF	390:391	OBJECTIVE Viscosupplementation has been used for decades to treat mild to moderate osteoarthritis, yet it is unknown if the lubricating function of different pathological synovial fluids (SF) vary, or if they respond differentially to viscosupplementation.
32105835	1	23	theme	different	350:358	arg1	fluids					382:387	different pathological synovial fluids	350:387	different pathological synovial fluids (SF)	350:392	OBJECTIVE Viscosupplementation has been used for decades to treat mild to moderate osteoarthritis, yet it is unknown if the lubricating function of different pathological synovial fluids (SF) vary, or if they respond differentially to viscosupplementation.
32105835	3	24	theme	cell	862:865	arg1	inflammatory					874:885	inflammatory	874:885	inflammatory	874:885	METHOD Human pathological SF was grouped by white blood cell count (inflammatory: >2000 cells/mm3, n = 6; non-inflammatory: <2000 cells/mm3, n = 6).
32105835	3	24	theme	cell	862:865	arg1	count					867:871	white blood cell count	850:871	white blood cell count (inflammatory: >2000 cells/mm3, n = 6; non-inflammatory: <2000 cells/mm3, n = 6)	850:952	METHOD Human pathological SF was grouped by white blood cell count (inflammatory: >2000 cells/mm3, n = 6; non-inflammatory: <2000 cells/mm3, n = 6).
32105835	3	25	theme	Human	813:817	arg1	SF					832:833	METHOD Human pathological SF	806:833	METHOD Human pathological SF	806:833	METHOD Human pathological SF was grouped by white blood cell count (inflammatory: >2000 cells/mm3, n = 6; non-inflammatory: <2000 cells/mm3, n = 6).
32105835	1	26	theme	synovial	373:380	arg1	SF					390:391	SF	390:391	SF	390:391	OBJECTIVE Viscosupplementation has been used for decades to treat mild to moderate osteoarthritis, yet it is unknown if the lubricating function of different pathological synovial fluids (SF) vary, or if they respond differentially to viscosupplementation.
32105835	1	26	theme	synovial	373:380	arg1	fluids					382:387	different pathological synovial fluids	350:387	different pathological synovial fluids (SF)	350:392	OBJECTIVE Viscosupplementation has been used for decades to treat mild to moderate osteoarthritis, yet it is unknown if the lubricating function of different pathological synovial fluids (SF) vary, or if they respond differentially to viscosupplementation.
32105835	9	27	dep	CONCLUSION	1819:1828	arg1	suggest					1844:1850	suggest	1844:1850	suggest that pathological SF is characterized by distinct tribological endotypes where SF lubricating behaviors are differentially modified by viscosupplementation and are identifiable by biomarkers	1844:2041	CONCLUSION These results suggest that pathological SF is characterized by distinct tribological endotypes where SF lubricating behaviors are differentially modified by viscosupplementation and are identifiable by biomarkers.
32105835	3	28	theme	white	850:854	arg1	inflammatory					874:885	inflammatory	874:885	inflammatory	874:885	METHOD Human pathological SF was grouped by white blood cell count (inflammatory: >2000 cells/mm3, n = 6; non-inflammatory: <2000 cells/mm3, n = 6).
32105835	3	28	theme	white	850:854	arg1	count					867:871	white blood cell count	850:871	white blood cell count (inflammatory: >2000 cells/mm3, n = 6; non-inflammatory: <2000 cells/mm3, n = 6)	850:952	METHOD Human pathological SF was grouped by white blood cell count (inflammatory: >2000 cells/mm3, n = 6; non-inflammatory: <2000 cells/mm3, n = 6).
32105835	7	29	from	strains	1619:1625	arg1	subset					1648:1653	this inflammatory subset	1630:1653	this inflammatory subset	1630:1653	A subset of inflammatory SF samples induced higher tissue strains, and HA supplementation was most effective at lowering friction and tissue strains in this inflammatory subset.
32105835	7	30	theme	HA	1549:1550	arg1	supplementation					1552:1566	HA supplementation	1549:1566	HA supplementation	1549:1566	A subset of inflammatory SF samples induced higher tissue strains, and HA supplementation was most effective at lowering friction and tissue strains in this inflammatory subset.
32105835	2	31	theme	friction	513:520	arg1	coefficients					522:533	the friction coefficients	509:533	the friction coefficients	509:533	The objectives of this study were to (i) evaluate the friction coefficients and induced shear strains in articular cartilage when lubricated with pathological SF, (ii) identify the effect of hyaluronic acid (HA) supplementation on friction coefficients and shear strains, and (iii) identify SF biomarkers that correlate with lubricating function.
32105835	5	32	theme	new	1105:1107	arg1	analyses					1133:1140	new, microscale rheological analyses	1105:1140	new, microscale rheological analyses by lubricating neonatal bovine cartilage explants with SF alone and in a 1:1 ratio with HA (Hymovis®)	1105:1242	Friction coefficients and local tissue shear strain measurements were coupled using new, microscale rheological analyses by lubricating neonatal bovine cartilage explants with SF alone and in a 1:1 ratio with HA (Hymovis®).
32105835	2	33	theme	HA	667:668	arg1	supplementation					671:685	hyaluronic acid (HA) supplementation	650:685	hyaluronic acid (HA) supplementation	650:685	The objectives of this study were to (i) evaluate the friction coefficients and induced shear strains in articular cartilage when lubricated with pathological SF, (ii) identify the effect of hyaluronic acid (HA) supplementation on friction coefficients and shear strains, and (iii) identify SF biomarkers that correlate with lubricating function.
32105835	6	34	theme	cartilage	1437:1445	arg1	R2 = 0.34					1466:1474	R2 = 0.34	1466:1474	R2 = 0.34	1466:1474	RESULTS Friction coefficients were not significantly different between the inflammatory and non-inflammatory pathologies (p = 0.09), and were poorly correlated with peak tissue strains at the cartilage articular surface (R2 = 0.34).
32105835	6	34	theme	cartilage	1437:1445	arg1	surface					1457:1463	the cartilage articular surface	1433:1463	the cartilage articular surface (R2 = 0.34)	1433:1475	RESULTS Friction coefficients were not significantly different between the inflammatory and non-inflammatory pathologies (p = 0.09), and were poorly correlated with peak tissue strains at the cartilage articular surface (R2 = 0.34).
32105835	2	35	theme	acid	661:664	arg1	supplementation					671:685	hyaluronic acid (HA) supplementation	650:685	hyaluronic acid (HA) supplementation	650:685	The objectives of this study were to (i) evaluate the friction coefficients and induced shear strains in articular cartilage when lubricated with pathological SF, (ii) identify the effect of hyaluronic acid (HA) supplementation on friction coefficients and shear strains, and (iii) identify SF biomarkers that correlate with lubricating function.
32105835	6	36	theme	tissue	1415:1420	arg1	strains					1422:1428	peak tissue strains	1410:1428	peak tissue strains at the cartilage articular surface (R2 = 0.34)	1410:1475	RESULTS Friction coefficients were not significantly different between the inflammatory and non-inflammatory pathologies (p = 0.09), and were poorly correlated with peak tissue strains at the cartilage articular surface (R2 = 0.34).
32105835	0	37	theme	pathological	35:46	arg1	fluid					63:67	pathological human synovial fluid	35:67	pathological human synovial fluid	35:67	Distinct tribological endotypes of pathological human synovial fluid reveal characteristic biomarkers and variation in efficacy of viscosupplementation at reducing local strains in articular cartilage.
32105835	7	38	theme	higher	1522:1527	arg1	strains					1536:1542	higher tissue strains	1522:1542	higher tissue strains	1522:1542	A subset of inflammatory SF samples induced higher tissue strains, and HA supplementation was most effective at lowering friction and tissue strains in this inflammatory subset.
32105835	9	39	mod	modified	1975:1982	arg1	behaviors					1946:1954	SF lubricating behaviors	1931:1954	SF lubricating behaviors	1931:1954	CONCLUSION These results suggest that pathological SF is characterized by distinct tribological endotypes where SF lubricating behaviors are differentially modified by viscosupplementation and are identifiable by biomarkers.
32105835	9	39	mod	modified	1975:1982	arg3	viscosupplementation					1987:2006	viscosupplementation	1987:2006	viscosupplementation	1987:2006	CONCLUSION These results suggest that pathological SF is characterized by distinct tribological endotypes where SF lubricating behaviors are differentially modified by viscosupplementation and are identifiable by biomarkers.
32105835	2	40	theme	study	482:486	arg1	objectives					463:472	The objectives	459:472	The objectives of this study	459:486	The objectives of this study were to (i) evaluate the friction coefficients and induced shear strains in articular cartilage when lubricated with pathological SF, (ii) identify the effect of hyaluronic acid (HA) supplementation on friction coefficients and shear strains, and (iii) identify SF biomarkers that correlate with lubricating function.
32105835	0	41	theme	synovial	54:61	arg1	fluid					63:67	pathological human synovial fluid	35:67	pathological human synovial fluid	35:67	Distinct tribological endotypes of pathological human synovial fluid reveal characteristic biomarkers and variation in efficacy of viscosupplementation at reducing local strains in articular cartilage.
32105835	5	42	theme	bovine	1166:1171	arg1	explants					1183:1190	neonatal bovine cartilage explants	1157:1190	neonatal bovine cartilage explants	1157:1190	Friction coefficients and local tissue shear strain measurements were coupled using new, microscale rheological analyses by lubricating neonatal bovine cartilage explants with SF alone and in a 1:1 ratio with HA (Hymovis®).
32105835	7	43	theme	samples	1506:1512	arg1	subset					1480:1485	A subset	1478:1485	A subset of inflammatory SF samples	1478:1512	A subset of inflammatory SF samples induced higher tissue strains, and HA supplementation was most effective at lowering friction and tissue strains in this inflammatory subset.
32105835	5	44	theme	Friction	1021:1028	arg1	coefficients					1030:1041	Friction coefficients	1021:1041	Friction coefficients	1021:1041	Friction coefficients and local tissue shear strain measurements were coupled using new, microscale rheological analyses by lubricating neonatal bovine cartilage explants with SF alone and in a 1:1 ratio with HA (Hymovis®).
32105835	7	45	theme	inflammatory	1490:1501	arg1	samples					1506:1512	inflammatory SF samples	1490:1512	inflammatory SF samples	1490:1512	A subset of inflammatory SF samples induced higher tissue strains, and HA supplementation was most effective at lowering friction and tissue strains in this inflammatory subset.
32105835	6	46	from	surface	1457:1463	arg1	strains					1422:1428	peak tissue strains	1410:1428	peak tissue strains at the cartilage articular surface (R2 = 0.34)	1410:1475	RESULTS Friction coefficients were not significantly different between the inflammatory and non-inflammatory pathologies (p = 0.09), and were poorly correlated with peak tissue strains at the cartilage articular surface (R2 = 0.34).
32105835	0	47	theme	fluid	63:67	arg1	endotypes					22:30	Distinct tribological endotypes	0:30	Distinct tribological endotypes of pathological human synovial fluid	0:67	Distinct tribological endotypes of pathological human synovial fluid reveal characteristic biomarkers and variation in efficacy of viscosupplementation at reducing local strains in articular cartilage.
32105835	9	48	theme	tribological	1902:1913	arg1	endotypes					1915:1923	distinct tribological endotypes	1893:1923	distinct tribological endotypes where SF lubricating behaviors are differentially modified by viscosupplementation and are identifiable by biomarkers	1893:2041	CONCLUSION These results suggest that pathological SF is characterized by distinct tribological endotypes where SF lubricating behaviors are differentially modified by viscosupplementation and are identifiable by biomarkers.
32105835	5	49	theme	neonatal	1157:1164	arg1	explants					1183:1190	neonatal bovine cartilage explants	1157:1190	neonatal bovine cartilage explants	1157:1190	Friction coefficients and local tissue shear strain measurements were coupled using new, microscale rheological analyses by lubricating neonatal bovine cartilage explants with SF alone and in a 1:1 ratio with HA (Hymovis®).
32105835	1	50	theme	moderate	276:283	arg1	osteoarthritis					285:298	mild to moderate osteoarthritis	268:298	mild to moderate osteoarthritis	268:298	OBJECTIVE Viscosupplementation has been used for decades to treat mild to moderate osteoarthritis, yet it is unknown if the lubricating function of different pathological synovial fluids (SF) vary, or if they respond differentially to viscosupplementation.
32105835	5	51	theme	rheological	1121:1131	arg1	analyses					1133:1140	new, microscale rheological analyses	1105:1140	new, microscale rheological analyses by lubricating neonatal bovine cartilage explants with SF alone and in a 1:1 ratio with HA (Hymovis®)	1105:1242	Friction coefficients and local tissue shear strain measurements were coupled using new, microscale rheological analyses by lubricating neonatal bovine cartilage explants with SF alone and in a 1:1 ratio with HA (Hymovis®).
32105835	8	52	theme	clear	1690:1694	arg1	relationships					1696:1708	clear relationships	1690:1708	clear relationships between polymorphonuclear neutrophil (PMN), IL-8, and lubricin concentrations with cartilage tissue strains	1690:1816	Across all pathologies there were clear relationships between polymorphonuclear neutrophil (PMN), IL-8, and lubricin concentrations with cartilage tissue strains.
32105835	9	53	theme	lubricating	1934:1944	arg1	behaviors					1946:1954	SF lubricating behaviors	1931:1954	SF lubricating behaviors	1931:1954	CONCLUSION These results suggest that pathological SF is characterized by distinct tribological endotypes where SF lubricating behaviors are differentially modified by viscosupplementation and are identifiable by biomarkers.
32105835	6	54	theme	RESULTS	1245:1251	arg1	coefficients					1262:1273	RESULTS Friction coefficients	1245:1273	RESULTS Friction coefficients	1245:1273	RESULTS Friction coefficients were not significantly different between the inflammatory and non-inflammatory pathologies (p = 0.09), and were poorly correlated with peak tissue strains at the cartilage articular surface (R2 = 0.34).
32105835	9	55	theme	pathological	1857:1868	arg1	SF					1870:1871	pathological SF	1857:1871	pathological SF	1857:1871	CONCLUSION These results suggest that pathological SF is characterized by distinct tribological endotypes where SF lubricating behaviors are differentially modified by viscosupplementation and are identifiable by biomarkers.
32105835	8	56	with	concentrations	1773:1786	arg1	strains					1810:1816	cartilage tissue strains	1793:1816	cartilage tissue strains	1793:1816	Across all pathologies there were clear relationships between polymorphonuclear neutrophil (PMN), IL-8, and lubricin concentrations with cartilage tissue strains.
32105835	8	57	theme	neutrophil	1736:1745	arg1	concentrations					1773:1786	polymorphonuclear neutrophil (PMN), IL-8, and lubricin concentrations	1718:1786	polymorphonuclear neutrophil (PMN), IL-8, and lubricin concentrations with cartilage tissue strains	1718:1816	Across all pathologies there were clear relationships between polymorphonuclear neutrophil (PMN), IL-8, and lubricin concentrations with cartilage tissue strains.
32105835	7	58	theme	inflammatory	1635:1646	arg1	subset					1648:1653	this inflammatory subset	1630:1653	this inflammatory subset	1630:1653	A subset of inflammatory SF samples induced higher tissue strains, and HA supplementation was most effective at lowering friction and tissue strains in this inflammatory subset.
32105835	2	59	theme	SF	750:751	arg1	biomarkers					753:762	SF biomarkers	750:762	SF biomarkers that correlate with lubricating function	750:803	The objectives of this study were to (i) evaluate the friction coefficients and induced shear strains in articular cartilage when lubricated with pathological SF, (ii) identify the effect of hyaluronic acid (HA) supplementation on friction coefficients and shear strains, and (iii) identify SF biomarkers that correlate with lubricating function.
32105835	0	60	theme	local	164:168	arg1	strains					170:176	local strains	164:176	local strains	164:176	Distinct tribological endotypes of pathological human synovial fluid reveal characteristic biomarkers and variation in efficacy of viscosupplementation at reducing local strains in articular cartilage.
32105835	5	61	theme	strain	1066:1071	arg1	measurements					1073:1084	local tissue shear strain measurements	1047:1084	local tissue shear strain measurements	1047:1084	Friction coefficients and local tissue shear strain measurements were coupled using new, microscale rheological analyses by lubricating neonatal bovine cartilage explants with SF alone and in a 1:1 ratio with HA (Hymovis®).
32105835	7	62	theme	tissue	1612:1617	arg1	strains					1619:1625	tissue strains	1612:1625	tissue strains	1612:1625	A subset of inflammatory SF samples induced higher tissue strains, and HA supplementation was most effective at lowering friction and tissue strains in this inflammatory subset.
32105835	2	63	theme	induced	539:545	arg1	strains					553:559	induced shear strains	539:559	induced shear strains	539:559	The objectives of this study were to (i) evaluate the friction coefficients and induced shear strains in articular cartilage when lubricated with pathological SF, (ii) identify the effect of hyaluronic acid (HA) supplementation on friction coefficients and shear strains, and (iii) identify SF biomarkers that correlate with lubricating function.
32105835	3	64	dep	inflammatory	874:885	arg1	n = 6					905:909	n = 6	905:909	n = 6	905:909	METHOD Human pathological SF was grouped by white blood cell count (inflammatory: >2000 cells/mm3, n = 6; non-inflammatory: <2000 cells/mm3, n = 6).
32105835	3	64	dep	inflammatory	874:885	arg1	n = 6					947:951	n = 6	947:951	n = 6	947:951	METHOD Human pathological SF was grouped by white blood cell count (inflammatory: >2000 cells/mm3, n = 6; non-inflammatory: <2000 cells/mm3, n = 6).
32105835	3	64	dep	inflammatory	874:885	arg1	>2000 cells/mm3					888:902	>2000 cells/mm3	888:902	>2000 cells/mm3	888:902	METHOD Human pathological SF was grouped by white blood cell count (inflammatory: >2000 cells/mm3, n = 6; non-inflammatory: <2000 cells/mm3, n = 6).
32105835	3	64	dep	inflammatory	874:885	arg1	non-inflammatory					912:927	non-inflammatory	912:927	non-inflammatory	912:927	METHOD Human pathological SF was grouped by white blood cell count (inflammatory: >2000 cells/mm3, n = 6; non-inflammatory: <2000 cells/mm3, n = 6).
32105835	8	65	theme	lubricin	1764:1771	arg1	concentrations					1773:1786	polymorphonuclear neutrophil (PMN), IL-8, and lubricin concentrations	1718:1786	polymorphonuclear neutrophil (PMN), IL-8, and lubricin concentrations with cartilage tissue strains	1718:1816	Across all pathologies there were clear relationships between polymorphonuclear neutrophil (PMN), IL-8, and lubricin concentrations with cartilage tissue strains.
32105835	2	66	theme	articular	564:572	arg1	cartilage					574:582	articular cartilage	564:582	articular cartilage	564:582	The objectives of this study were to (i) evaluate the friction coefficients and induced shear strains in articular cartilage when lubricated with pathological SF, (ii) identify the effect of hyaluronic acid (HA) supplementation on friction coefficients and shear strains, and (iii) identify SF biomarkers that correlate with lubricating function.
32105835	1	67	dep	moderate	276:283	arg1	to					273:274	to	273:274	to	273:274	OBJECTIVE Viscosupplementation has been used for decades to treat mild to moderate osteoarthritis, yet it is unknown if the lubricating function of different pathological synovial fluids (SF) vary, or if they respond differentially to viscosupplementation.
32105835	0	68	theme	characteristic	76:89	arg1	biomarkers					91:100	characteristic biomarkers	76:100	characteristic biomarkers	76:100	Distinct tribological endotypes of pathological human synovial fluid reveal characteristic biomarkers and variation in efficacy of viscosupplementation at reducing local strains in articular cartilage.
32105835	5	69	theme	tissue	1053:1058	arg1	measurements					1073:1084	local tissue shear strain measurements	1047:1084	local tissue shear strain measurements	1047:1084	Friction coefficients and local tissue shear strain measurements were coupled using new, microscale rheological analyses by lubricating neonatal bovine cartilage explants with SF alone and in a 1:1 ratio with HA (Hymovis®).
32105835	2	70	theme	shear	716:720	arg1	strains					722:728	shear strains	716:728	shear strains	716:728	The objectives of this study were to (i) evaluate the friction coefficients and induced shear strains in articular cartilage when lubricated with pathological SF, (ii) identify the effect of hyaluronic acid (HA) supplementation on friction coefficients and shear strains, and (iii) identify SF biomarkers that correlate with lubricating function.
32105835	1	71	theme	pathological	360:371	arg1	SF					390:391	SF	390:391	SF	390:391	OBJECTIVE Viscosupplementation has been used for decades to treat mild to moderate osteoarthritis, yet it is unknown if the lubricating function of different pathological synovial fluids (SF) vary, or if they respond differentially to viscosupplementation.
32105835	1	71	theme	pathological	360:371	arg1	fluids					382:387	different pathological synovial fluids	350:387	different pathological synovial fluids (SF)	350:392	OBJECTIVE Viscosupplementation has been used for decades to treat mild to moderate osteoarthritis, yet it is unknown if the lubricating function of different pathological synovial fluids (SF) vary, or if they respond differentially to viscosupplementation.
32105835	7	72	from	friction	1599:1606	arg1	subset					1648:1653	this inflammatory subset	1630:1653	this inflammatory subset	1630:1653	A subset of inflammatory SF samples induced higher tissue strains, and HA supplementation was most effective at lowering friction and tissue strains in this inflammatory subset.
32105835	3	73	dep	non-inflammatory	912:927	arg1	<2000 cells/mm3					930:944	<2000 cells/mm3	930:944	<2000 cells/mm3	930:944	METHOD Human pathological SF was grouped by white blood cell count (inflammatory: >2000 cells/mm3, n = 6; non-inflammatory: <2000 cells/mm3, n = 6).
32105835	4	74	theme	Compositional	955:967	arg1	analyses					969:976	Compositional analyses	955:976	Compositional analyses for lubricin and cytokines	955:1003	Compositional analyses for lubricin and cytokines were performed.
32105835	2	75	theme	hyaluronic	650:659	arg1	supplementation					671:685	hyaluronic acid (HA) supplementation	650:685	hyaluronic acid (HA) supplementation	650:685	The objectives of this study were to (i) evaluate the friction coefficients and induced shear strains in articular cartilage when lubricated with pathological SF, (ii) identify the effect of hyaluronic acid (HA) supplementation on friction coefficients and shear strains, and (iii) identify SF biomarkers that correlate with lubricating function.
32105835	3	76	theme	pathological	819:830	arg1	SF					832:833	METHOD Human pathological SF	806:833	METHOD Human pathological SF	806:833	METHOD Human pathological SF was grouped by white blood cell count (inflammatory: >2000 cells/mm3, n = 6; non-inflammatory: <2000 cells/mm3, n = 6).
32105835	1	77	theme	fluids	382:387	arg1	function					338:345	the lubricating function	322:345	the lubricating function of different pathological synovial fluids (SF)	322:392	OBJECTIVE Viscosupplementation has been used for decades to treat mild to moderate osteoarthritis, yet it is unknown if the lubricating function of different pathological synovial fluids (SF) vary, or if they respond differentially to viscosupplementation.
32105835	3	78	theme	blood	856:860	arg1	inflammatory					874:885	inflammatory	874:885	inflammatory	874:885	METHOD Human pathological SF was grouped by white blood cell count (inflammatory: >2000 cells/mm3, n = 6; non-inflammatory: <2000 cells/mm3, n = 6).
32105835	3	78	theme	blood	856:860	arg1	count					867:871	white blood cell count	850:871	white blood cell count (inflammatory: >2000 cells/mm3, n = 6; non-inflammatory: <2000 cells/mm3, n = 6)	850:952	METHOD Human pathological SF was grouped by white blood cell count (inflammatory: >2000 cells/mm3, n = 6; non-inflammatory: <2000 cells/mm3, n = 6).
32105835	3	79	theme	METHOD	806:811	arg1	SF					832:833	METHOD Human pathological SF	806:833	METHOD Human pathological SF	806:833	METHOD Human pathological SF was grouped by white blood cell count (inflammatory: >2000 cells/mm3, n = 6; non-inflammatory: <2000 cells/mm3, n = 6).
32105835	0	80	theme	tribological	9:20	arg1	endotypes					22:30	Distinct tribological endotypes	0:30	Distinct tribological endotypes of pathological human synovial fluid	0:67	Distinct tribological endotypes of pathological human synovial fluid reveal characteristic biomarkers and variation in efficacy of viscosupplementation at reducing local strains in articular cartilage.
32105835	0	81	theme	human	48:52	arg1	fluid					63:67	pathological human synovial fluid	35:67	pathological human synovial fluid	35:67	Distinct tribological endotypes of pathological human synovial fluid reveal characteristic biomarkers and variation in efficacy of viscosupplementation at reducing local strains in articular cartilage.
32105835	5	82	dep	new	1105:1107	arg1	microscale					1110:1119	microscale	1110:1119	microscale	1110:1119	Friction coefficients and local tissue shear strain measurements were coupled using new, microscale rheological analyses by lubricating neonatal bovine cartilage explants with SF alone and in a 1:1 ratio with HA (Hymovis®).
32105835	6	83	theme	peak	1410:1413	arg1	strains					1422:1428	peak tissue strains	1410:1428	peak tissue strains at the cartilage articular surface (R2 = 0.34)	1410:1475	RESULTS Friction coefficients were not significantly different between the inflammatory and non-inflammatory pathologies (p = 0.09), and were poorly correlated with peak tissue strains at the cartilage articular surface (R2 = 0.34).
32105835	1	84	theme	lubricating	326:336	arg1	function					338:345	the lubricating function	322:345	the lubricating function of different pathological synovial fluids (SF)	322:392	OBJECTIVE Viscosupplementation has been used for decades to treat mild to moderate osteoarthritis, yet it is unknown if the lubricating function of different pathological synovial fluids (SF) vary, or if they respond differentially to viscosupplementation.
32105835	7	85	theme	tissue	1529:1534	arg1	strains					1536:1542	higher tissue strains	1522:1542	higher tissue strains	1522:1542	A subset of inflammatory SF samples induced higher tissue strains, and HA supplementation was most effective at lowering friction and tissue strains in this inflammatory subset.
32105835	2	86	dep	identify	741:748	arg1	iii					736:738	iii	736:738	iii	736:738	The objectives of this study were to (i) evaluate the friction coefficients and induced shear strains in articular cartilage when lubricated with pathological SF, (ii) identify the effect of hyaluronic acid (HA) supplementation on friction coefficients and shear strains, and (iii) identify SF biomarkers that correlate with lubricating function.
32105835	5	87	theme	cartilage	1173:1181	arg1	explants					1183:1190	neonatal bovine cartilage explants	1157:1190	neonatal bovine cartilage explants	1157:1190	Friction coefficients and local tissue shear strain measurements were coupled using new, microscale rheological analyses by lubricating neonatal bovine cartilage explants with SF alone and in a 1:1 ratio with HA (Hymovis®).
32105835	2	88	dep	evaluate	500:507	arg1	i					497:497	i	497:497	i	497:497	The objectives of this study were to (i) evaluate the friction coefficients and induced shear strains in articular cartilage when lubricated with pathological SF, (ii) identify the effect of hyaluronic acid (HA) supplementation on friction coefficients and shear strains, and (iii) identify SF biomarkers that correlate with lubricating function.
32105835	2	89	theme	lubricating	784:794	arg1	function					796:803	lubricating function	784:803	lubricating function	784:803	The objectives of this study were to (i) evaluate the friction coefficients and induced shear strains in articular cartilage when lubricated with pathological SF, (ii) identify the effect of hyaluronic acid (HA) supplementation on friction coefficients and shear strains, and (iii) identify SF biomarkers that correlate with lubricating function.
32105835	7	90	theme	SF	1503:1504	arg1	samples					1506:1512	inflammatory SF samples	1490:1512	inflammatory SF samples	1490:1512	A subset of inflammatory SF samples induced higher tissue strains, and HA supplementation was most effective at lowering friction and tissue strains in this inflammatory subset.
32105835	0	91	theme	viscosupplementation	131:150	arg1	efficacy					119:126	efficacy	119:126	efficacy of viscosupplementation	119:150	Distinct tribological endotypes of pathological human synovial fluid reveal characteristic biomarkers and variation in efficacy of viscosupplementation at reducing local strains in articular cartilage.
32105835	9	92	theme	distinct	1893:1900	arg1	endotypes					1915:1923	distinct tribological endotypes	1893:1923	distinct tribological endotypes where SF lubricating behaviors are differentially modified by viscosupplementation and are identifiable by biomarkers	1893:2041	CONCLUSION These results suggest that pathological SF is characterized by distinct tribological endotypes where SF lubricating behaviors are differentially modified by viscosupplementation and are identifiable by biomarkers.
32105835	5	93	with	ratio	1219:1223	arg1	Hymovis®					1234:1241	Hymovis®	1234:1241	Hymovis®	1234:1241	Friction coefficients and local tissue shear strain measurements were coupled using new, microscale rheological analyses by lubricating neonatal bovine cartilage explants with SF alone and in a 1:1 ratio with HA (Hymovis®).
32105835	5	93	with	ratio	1219:1223	arg1	HA					1230:1231	HA	1230:1231	HA (Hymovis®)	1230:1242	Friction coefficients and local tissue shear strain measurements were coupled using new, microscale rheological analyses by lubricating neonatal bovine cartilage explants with SF alone and in a 1:1 ratio with HA (Hymovis®).
32105835	6	94	theme	articular	1447:1455	arg1	R2 = 0.34					1466:1474	R2 = 0.34	1466:1474	R2 = 0.34	1466:1474	RESULTS Friction coefficients were not significantly different between the inflammatory and non-inflammatory pathologies (p = 0.09), and were poorly correlated with peak tissue strains at the cartilage articular surface (R2 = 0.34).
32105835	6	94	theme	articular	1447:1455	arg1	surface					1457:1463	the cartilage articular surface	1433:1463	the cartilage articular surface (R2 = 0.34)	1433:1475	RESULTS Friction coefficients were not significantly different between the inflammatory and non-inflammatory pathologies (p = 0.09), and were poorly correlated with peak tissue strains at the cartilage articular surface (R2 = 0.34).
32113768	4	0	theme	mean	744:747	arg1	deviation					760:768	mean ± standard deviation	744:768	mean ± standard deviation	744:768	For this study 24 individual dairy cows (parity 2.5 ± 0.5; mean ± standard deviation) were studied in lactation wk 2.
32113768	12	1	located	detected	1492:1499	arg1	plasma					1509:1514	plasma	1509:1514	plasma	1509:1514	Nine metabolites detected in both plasma and milk were correlated with each other and with energy balance.
32113768	12	1	located	detected	1492:1499	arg2	metabolites					1480:1490	Nine metabolites	1475:1490	Nine metabolites detected in both plasma and milk	1475:1523	Nine metabolites detected in both plasma and milk were correlated with each other and with energy balance.
32113768	12	1	located	detected	1492:1499	arg1	milk					1520:1523	milk	1520:1523	milk	1520:1523	Nine metabolites detected in both plasma and milk were correlated with each other and with energy balance.
32113768	4	2	theme	standard	751:758	arg1	deviation					760:768	mean ± standard deviation	744:768	mean ± standard deviation	744:768	For this study 24 individual dairy cows (parity 2.5 ± 0.5; mean ± standard deviation) were studied in lactation wk 2.
32113768	10	3	theme	milk	1330:1333	arg1	metabolites					1335:1345	all milk metabolites	1326:1345	all milk metabolites measured	1326:1354	From all milk metabolites measured, 30 were correlated with energy balance.
32113768	15	4	from	metabolites	2001:2011	arg1	cows					2117:2120	lactating dairy cows	2101:2120	lactating dairy cows	2101:2120	In addition to the classical indicators for energy balance (e.g., β-hydroxybutyrate, acetone, and glucose), the current study presents some new metabolites (e.g., glycine in plasma and milk; kynurenine, panthothenate, or arginine in plasma) in lactating dairy cows that are related to energy balance and may be of interest as new indicators for energy balance.
32113768	2	5	from	profile	307:313	arg1	plasma					285:290	plasma	285:290	plasma	285:290	However, the relationships among energy balance, metabolic profile in plasma, and metabolic profile in milk have not been reported.
32113768	2	5	from	profile	307:313	arg1	milk					318:321	milk	318:321	milk	318:321	However, the relationships among energy balance, metabolic profile in plasma, and metabolic profile in milk have not been reported.
32113768	6	6	dep	composition	947:957	arg1	protein					965:971	protein	965:971	protein	965:971	Milk composition (fat, protein, and lactose) and net energy balance were calculated.
32113768	6	6	dep	composition	947:957	arg1	lactose					978:984	lactose	978:984	lactose	978:984	Milk composition (fat, protein, and lactose) and net energy balance were calculated.
32113768	6	6	dep	composition	947:957	arg1	fat					960:962	fat	960:962	fat	960:962	Milk composition (fat, protein, and lactose) and net energy balance were calculated.
32113768	2	7	theme	energy	248:253	arg1	balance					255:261	energy balance	248:261	energy balance	248:261	However, the relationships among energy balance, metabolic profile in plasma, and metabolic profile in milk have not been reported.
32113768	1	8	theme	early	153:157	arg1	lactation					159:167	early lactation	153:167	early lactation	153:167	Negative energy balance in dairy cows in early lactation is related to alteration of metabolic status.
32113768	9	9	from	bone	1272:1275	arg1	gluconeogenesis					1304:1318	gluconeogenesis	1304:1318	gluconeogenesis	1304:1318	These plasma metabolites were related to body reserve mobilization from body fat, muscle, and bone; increased blood flow; and gluconeogenesis.
32113768	9	9	from	bone	1272:1275	arg1	mobilization					1232:1243	body reserve mobilization	1219:1243	body reserve mobilization from body fat, muscle, and bone	1219:1275	These plasma metabolites were related to body reserve mobilization from body fat, muscle, and bone; increased blood flow; and gluconeogenesis.
32113768	9	9	from	bone	1272:1275	arg1	flow					1294:1297	increased blood flow	1278:1297	increased blood flow	1278:1297	These plasma metabolites were related to body reserve mobilization from body fat, muscle, and bone; increased blood flow; and gluconeogenesis.
32113768	4	10	theme	lactation	787:795	arg1	wk					797:798	lactation wk 2	787:800	lactation wk 2	787:800	For this study 24 individual dairy cows (parity 2.5 ± 0.5; mean ± standard deviation) were studied in lactation wk 2.
32113768	9	11	theme	body	1250:1253	arg1	fat					1255:1257	body fat	1250:1257	body fat	1250:1257	These plasma metabolites were related to body reserve mobilization from body fat, muscle, and bone; increased blood flow; and gluconeogenesis.
32113768	0	12	from	milk	73:76	arg1	lactation					101:109	early lactation	95:109	early lactation	95:109	Relationship between energy balance and metabolic profiles in plasma and milk of dairy cows in early lactation.
32113768	10	13	theme	energy	1381:1386	arg1	balance					1388:1394	energy balance	1381:1394	energy balance	1381:1394	From all milk metabolites measured, 30 were correlated with energy balance.
32113768	3	14	theme	milk	679:682	arg1	profiles					656:663	the metabolic profiles	642:663	the metabolic profiles of plasma and milk	642:682	In this study our aims were: (1) to reveal the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS); and (2) to investigate the relationship between energy balance and the metabolic profiles of plasma and milk.
32113768	3	14	theme	milk	679:682	arg1	balance					630:636	energy balance	623:636	energy balance	623:636	In this study our aims were: (1) to reveal the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS); and (2) to investigate the relationship between energy balance and the metabolic profiles of plasma and milk.
32113768	3	15	theme	chromatography	515:528	arg1	LC-MS					567:571	LC-MS	567:571	LC-MS	567:571	In this study our aims were: (1) to reveal the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS); and (2) to investigate the relationship between energy balance and the metabolic profiles of plasma and milk.
32113768	3	15	theme	chromatography	515:528	arg1	spectrometry					553:564	liquid chromatography triple quadrupole mass spectrometry	508:564	liquid chromatography triple quadrupole mass spectrometry (LC-MS)	508:572	In this study our aims were: (1) to reveal the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS); and (2) to investigate the relationship between energy balance and the metabolic profiles of plasma and milk.
32113768	15	16	theme	energy	1901:1906	arg1	balance					1908:1914	energy balance	1901:1914	energy balance	1901:1914	In addition to the classical indicators for energy balance (e.g., β-hydroxybutyrate, acetone, and glucose), the current study presents some new metabolites (e.g., glycine in plasma and milk; kynurenine, panthothenate, or arginine in plasma) in lactating dairy cows that are related to energy balance and may be of interest as new indicators for energy balance.
32113768	0	17	from	plasma	62:67	arg1	lactation					101:109	early lactation	95:109	early lactation	95:109	Relationship between energy balance and metabolic profiles in plasma and milk of dairy cows in early lactation.
32113768	15	18	dep	β-hydroxybutyrate	1923:1939	arg1	e.g.					1917:1920	e.g.	1917:1920	e.g.	1917:1920	In addition to the classical indicators for energy balance (e.g., β-hydroxybutyrate, acetone, and glucose), the current study presents some new metabolites (e.g., glycine in plasma and milk; kynurenine, panthothenate, or arginine in plasma) in lactating dairy cows that are related to energy balance and may be of interest as new indicators for energy balance.
32113768	3	19	theme	plasma	668:673	arg1	profiles					656:663	the metabolic profiles	642:663	the metabolic profiles of plasma and milk	642:682	In this study our aims were: (1) to reveal the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS); and (2) to investigate the relationship between energy balance and the metabolic profiles of plasma and milk.
32113768	3	19	theme	plasma	668:673	arg1	balance					630:636	energy balance	623:636	energy balance	623:636	In this study our aims were: (1) to reveal the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS); and (2) to investigate the relationship between energy balance and the metabolic profiles of plasma and milk.
32113768	15	20	theme	current	1969:1975	arg1	study					1977:1981	the current study	1965:1981	the current study	1965:1981	In addition to the classical indicators for energy balance (e.g., β-hydroxybutyrate, acetone, and glucose), the current study presents some new metabolites (e.g., glycine in plasma and milk; kynurenine, panthothenate, or arginine in plasma) in lactating dairy cows that are related to energy balance and may be of interest as new indicators for energy balance.
32113768	2	21	from	balance	255:261	arg1	plasma					285:290	plasma	285:290	plasma	285:290	However, the relationships among energy balance, metabolic profile in plasma, and metabolic profile in milk have not been reported.
32113768	2	21	from	balance	255:261	arg1	milk					318:321	milk	318:321	milk	318:321	However, the relationships among energy balance, metabolic profile in plasma, and metabolic profile in milk have not been reported.
32113768	15	22	from	kynurenine	2048:2057	arg1	plasma					2031:2036	plasma	2031:2036	plasma	2031:2036	In addition to the classical indicators for energy balance (e.g., β-hydroxybutyrate, acetone, and glucose), the current study presents some new metabolites (e.g., glycine in plasma and milk; kynurenine, panthothenate, or arginine in plasma) in lactating dairy cows that are related to energy balance and may be of interest as new indicators for energy balance.
32113768	15	22	from	kynurenine	2048:2057	arg1	plasma					2090:2095	plasma	2090:2095	plasma	2090:2095	In addition to the classical indicators for energy balance (e.g., β-hydroxybutyrate, acetone, and glucose), the current study presents some new metabolites (e.g., glycine in plasma and milk; kynurenine, panthothenate, or arginine in plasma) in lactating dairy cows that are related to energy balance and may be of interest as new indicators for energy balance.
32113768	15	22	from	kynurenine	2048:2057	arg1	milk					2042:2045	milk	2042:2045	milk	2042:2045	In addition to the classical indicators for energy balance (e.g., β-hydroxybutyrate, acetone, and glucose), the current study presents some new metabolites (e.g., glycine in plasma and milk; kynurenine, panthothenate, or arginine in plasma) in lactating dairy cows that are related to energy balance and may be of interest as new indicators for energy balance.
32113768	6	23	theme	net	991:993	arg1	balance					1002:1008	net energy balance	991:1008	net energy balance	991:1008	Milk composition (fat, protein, and lactose) and net energy balance were calculated.
32113768	13	24	theme	one-carbon	1671:1680	arg1	metabolism					1682:1691	one-carbon metabolism	1671:1691	one-carbon metabolism	1671:1691	These metabolites were mainly related to hyperketonemia; β-oxidation of fatty acids; and one-carbon metabolism.
32113768	3	25	theme	magnetic	468:475	arg1	NMR					488:490	NMR	488:490	NMR	488:490	In this study our aims were: (1) to reveal the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS); and (2) to investigate the relationship between energy balance and the metabolic profiles of plasma and milk.
32113768	3	25	theme	magnetic	468:475	arg1	resonance					477:485	nuclear magnetic resonance	460:485	nuclear magnetic resonance (NMR)	460:491	In this study our aims were: (1) to reveal the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS); and (2) to investigate the relationship between energy balance and the metabolic profiles of plasma and milk.
32113768	1	26	theme	energy	121:126	arg1	balance					128:134	Negative energy balance	112:134	Negative energy balance in dairy cows in early lactation	112:167	Negative energy balance in dairy cows in early lactation is related to alteration of metabolic status.
32113768	15	27	from	panthothenate	2060:2072	arg1	plasma					2031:2036	plasma	2031:2036	plasma	2031:2036	In addition to the classical indicators for energy balance (e.g., β-hydroxybutyrate, acetone, and glucose), the current study presents some new metabolites (e.g., glycine in plasma and milk; kynurenine, panthothenate, or arginine in plasma) in lactating dairy cows that are related to energy balance and may be of interest as new indicators for energy balance.
32113768	15	27	from	panthothenate	2060:2072	arg1	plasma					2090:2095	plasma	2090:2095	plasma	2090:2095	In addition to the classical indicators for energy balance (e.g., β-hydroxybutyrate, acetone, and glucose), the current study presents some new metabolites (e.g., glycine in plasma and milk; kynurenine, panthothenate, or arginine in plasma) in lactating dairy cows that are related to energy balance and may be of interest as new indicators for energy balance.
32113768	15	27	from	panthothenate	2060:2072	arg1	milk					2042:2045	milk	2042:2045	milk	2042:2045	In addition to the classical indicators for energy balance (e.g., β-hydroxybutyrate, acetone, and glucose), the current study presents some new metabolites (e.g., glycine in plasma and milk; kynurenine, panthothenate, or arginine in plasma) in lactating dairy cows that are related to energy balance and may be of interest as new indicators for energy balance.
32113768	3	28	theme	quadrupole	537:546	arg1	LC-MS					567:571	LC-MS	567:571	LC-MS	567:571	In this study our aims were: (1) to reveal the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS); and (2) to investigate the relationship between energy balance and the metabolic profiles of plasma and milk.
32113768	3	28	theme	quadrupole	537:546	arg1	spectrometry					553:564	liquid chromatography triple quadrupole mass spectrometry	508:564	liquid chromatography triple quadrupole mass spectrometry (LC-MS)	508:572	In this study our aims were: (1) to reveal the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS); and (2) to investigate the relationship between energy balance and the metabolic profiles of plasma and milk.
32113768	9	29	theme	increased	1278:1286	arg1	flow					1294:1297	increased blood flow	1278:1297	increased blood flow	1278:1297	These plasma metabolites were related to body reserve mobilization from body fat, muscle, and bone; increased blood flow; and gluconeogenesis.
32113768	13	30	theme	fatty	1654:1658	arg1	acids					1660:1664	fatty acids	1654:1664	fatty acids	1654:1664	These metabolites were mainly related to hyperketonemia; β-oxidation of fatty acids; and one-carbon metabolism.
32113768	0	31	from	Relationship	0:11	arg1	milk					73:76	milk	73:76	milk	73:76	Relationship between energy balance and metabolic profiles in plasma and milk of dairy cows in early lactation.
32113768	0	31	from	Relationship	0:11	arg1	plasma					62:67	plasma	62:67	plasma	62:67	Relationship between energy balance and metabolic profiles in plasma and milk of dairy cows in early lactation.
32113768	3	32	from	resonance	477:485	arg1	results					447:453	results	447:453	results from nuclear magnetic resonance (NMR)	447:491	In this study our aims were: (1) to reveal the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS); and (2) to investigate the relationship between energy balance and the metabolic profiles of plasma and milk.
32113768	0	33	theme	dairy	81:85	arg1	cows					87:90	dairy cows	81:90	dairy cows	81:90	Relationship between energy balance and metabolic profiles in plasma and milk of dairy cows in early lactation.
32113768	5	34	theme	±	898:898	arg1	deviation					909:917	mean ± standard deviation	893:917	mean ± standard deviation	893:917	Body weight (mean ± standard deviation; 627.4 ± 56.4 kg) and milk yield (28.1 ± 6.7 kg/d; mean ± standard deviation) were monitored daily.
32113768	1	35	from	balance	128:134	arg1	lactation					159:167	early lactation	153:167	early lactation	153:167	Negative energy balance in dairy cows in early lactation is related to alteration of metabolic status.
32113768	1	35	from	balance	128:134	arg1	cows					145:148	dairy cows	139:148	dairy cows	139:148	Negative energy balance in dairy cows in early lactation is related to alteration of metabolic status.
32113768	5	36	theme	±	821:821	arg1	weight					808:813	Body weight	803:813	Body weight (mean ± standard deviation; 627.4 ± 56.4 kg)	803:858	Body weight (mean ± standard deviation; 627.4 ± 56.4 kg) and milk yield (28.1 ± 6.7 kg/d; mean ± standard deviation) were monitored daily.
32113768	5	36	theme	±	821:821	arg1	deviation					832:840	mean ± standard deviation	816:840	mean ± standard deviation	816:840	Body weight (mean ± standard deviation; 627.4 ± 56.4 kg) and milk yield (28.1 ± 6.7 kg/d; mean ± standard deviation) were monitored daily.
32113768	15	37	theme	lactating	2101:2109	arg1	cows					2117:2120	lactating dairy cows	2101:2120	lactating dairy cows	2101:2120	In addition to the classical indicators for energy balance (e.g., β-hydroxybutyrate, acetone, and glucose), the current study presents some new metabolites (e.g., glycine in plasma and milk; kynurenine, panthothenate, or arginine in plasma) in lactating dairy cows that are related to energy balance and may be of interest as new indicators for energy balance.
32113768	15	38	theme	energy	2202:2207	arg1	balance					2209:2215	energy balance	2202:2215	energy balance	2202:2215	In addition to the classical indicators for energy balance (e.g., β-hydroxybutyrate, acetone, and glucose), the current study presents some new metabolites (e.g., glycine in plasma and milk; kynurenine, panthothenate, or arginine in plasma) in lactating dairy cows that are related to energy balance and may be of interest as new indicators for energy balance.
32113768	3	39	from	spectrometry	553:564	arg1	data					498:501	data	498:501	data from liquid chromatography triple quadrupole mass spectrometry (LC-MS)	498:572	In this study our aims were: (1) to reveal the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS); and (2) to investigate the relationship between energy balance and the metabolic profiles of plasma and milk.
32113768	4	40	dep	±	737:737	arg1	deviation					760:768	mean ± standard deviation	744:768	mean ± standard deviation	744:768	For this study 24 individual dairy cows (parity 2.5 ± 0.5; mean ± standard deviation) were studied in lactation wk 2.
32113768	5	41	theme	Body	803:806	arg1	weight					808:813	Body weight	803:813	Body weight (mean ± standard deviation; 627.4 ± 56.4 kg)	803:858	Body weight (mean ± standard deviation; 627.4 ± 56.4 kg) and milk yield (28.1 ± 6.7 kg/d; mean ± standard deviation) were monitored daily.
32113768	5	41	theme	Body	803:806	arg1	deviation					832:840	mean ± standard deviation	816:840	mean ± standard deviation	816:840	Body weight (mean ± standard deviation; 627.4 ± 56.4 kg) and milk yield (28.1 ± 6.7 kg/d; mean ± standard deviation) were monitored daily.
32113768	15	42	dep	indicators	1886:1895	arg1	addition					1860:1867	addition	1860:1867	addition	1860:1867	In addition to the classical indicators for energy balance (e.g., β-hydroxybutyrate, acetone, and glucose), the current study presents some new metabolites (e.g., glycine in plasma and milk; kynurenine, panthothenate, or arginine in plasma) in lactating dairy cows that are related to energy balance and may be of interest as new indicators for energy balance.
32113768	14	43	theme	early	1840:1844	arg1	lactation					1846:1854	early lactation	1840:1854	early lactation	1840:1854	The metabolic profiles of plasma and milk provide an in-depth insight into the physiological pathways of dairy cows in negative energy balance in early lactation.
32113768	0	44	theme	energy	21:26	arg1	balance					28:34	energy balance	21:34	energy balance	21:34	Relationship between energy balance and metabolic profiles in plasma and milk of dairy cows in early lactation.
32113768	14	45	from	balance	1829:1835	arg1	lactation					1846:1854	early lactation	1840:1854	early lactation	1840:1854	The metabolic profiles of plasma and milk provide an in-depth insight into the physiological pathways of dairy cows in negative energy balance in early lactation.
32113768	4	46	theme	dairy	714:718	arg1	±					737:737	parity 2.5 ± 0.5	726:741	parity 2.5 ± 0.5	726:741	For this study 24 individual dairy cows (parity 2.5 ± 0.5; mean ± standard deviation) were studied in lactation wk 2.
32113768	4	46	theme	dairy	714:718	arg1	cows					720:723	24 individual dairy cows	700:723	24 individual dairy cows (parity 2.5 ± 0.5; mean ± standard deviation)	700:769	For this study 24 individual dairy cows (parity 2.5 ± 0.5; mean ± standard deviation) were studied in lactation wk 2.
32113768	14	47	theme	milk	1731:1734	arg1	profiles					1708:1715	The metabolic profiles	1694:1715	The metabolic profiles of plasma and milk	1694:1734	The metabolic profiles of plasma and milk provide an in-depth insight into the physiological pathways of dairy cows in negative energy balance in early lactation.
32113768	9	48	from	fat	1255:1257	arg1	gluconeogenesis					1304:1318	gluconeogenesis	1304:1318	gluconeogenesis	1304:1318	These plasma metabolites were related to body reserve mobilization from body fat, muscle, and bone; increased blood flow; and gluconeogenesis.
32113768	9	48	from	fat	1255:1257	arg1	mobilization					1232:1243	body reserve mobilization	1219:1243	body reserve mobilization from body fat, muscle, and bone	1219:1275	These plasma metabolites were related to body reserve mobilization from body fat, muscle, and bone; increased blood flow; and gluconeogenesis.
32113768	9	48	from	fat	1255:1257	arg1	flow					1294:1297	increased blood flow	1278:1297	increased blood flow	1278:1297	These plasma metabolites were related to body reserve mobilization from body fat, muscle, and bone; increased blood flow; and gluconeogenesis.
32113768	14	49	theme	negative	1813:1820	arg1	balance					1829:1835	negative energy balance	1813:1835	negative energy balance in early lactation	1813:1854	The metabolic profiles of plasma and milk provide an in-depth insight into the physiological pathways of dairy cows in negative energy balance in early lactation.
32113768	3	50	theme	plasma	416:421	arg1	profiles					404:411	the metabolic profiles	390:411	the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS)	390:572	In this study our aims were: (1) to reveal the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS); and (2) to investigate the relationship between energy balance and the metabolic profiles of plasma and milk.
32113768	15	51	dep	glycine	2020:2026	arg1	e.g.					2014:2017	e.g.	2014:2017	e.g.	2014:2017	In addition to the classical indicators for energy balance (e.g., β-hydroxybutyrate, acetone, and glucose), the current study presents some new metabolites (e.g., glycine in plasma and milk; kynurenine, panthothenate, or arginine in plasma) in lactating dairy cows that are related to energy balance and may be of interest as new indicators for energy balance.
32113768	14	52	theme	plasma	1720:1725	arg1	profiles					1708:1715	The metabolic profiles	1694:1715	The metabolic profiles of plasma and milk	1694:1734	The metabolic profiles of plasma and milk provide an in-depth insight into the physiological pathways of dairy cows in negative energy balance in early lactation.
32113768	14	53	theme	cows	1805:1808	arg1	pathways					1787:1794	the physiological pathways	1769:1794	the physiological pathways of dairy cows in negative energy balance in early lactation	1769:1854	The metabolic profiles of plasma and milk provide an in-depth insight into the physiological pathways of dairy cows in negative energy balance in early lactation.
32113768	4	54	theme	individual	703:712	arg1	±					737:737	parity 2.5 ± 0.5	726:741	parity 2.5 ± 0.5	726:741	For this study 24 individual dairy cows (parity 2.5 ± 0.5; mean ± standard deviation) were studied in lactation wk 2.
32113768	4	54	theme	individual	703:712	arg1	cows					720:723	24 individual dairy cows	700:723	24 individual dairy cows (parity 2.5 ± 0.5; mean ± standard deviation)	700:769	For this study 24 individual dairy cows (parity 2.5 ± 0.5; mean ± standard deviation) were studied in lactation wk 2.
32113768	2	55	theme	metabolic	297:305	arg1	profile					307:313	metabolic profile	297:313	metabolic profile in milk	297:321	However, the relationships among energy balance, metabolic profile in plasma, and metabolic profile in milk have not been reported.
32113768	1	56	theme	metabolic	197:205	arg1	status					207:212	metabolic status	197:212	metabolic status	197:212	Negative energy balance in dairy cows in early lactation is related to alteration of metabolic status.
32113768	3	57	theme	nuclear	460:466	arg1	NMR					488:490	NMR	488:490	NMR	488:490	In this study our aims were: (1) to reveal the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS); and (2) to investigate the relationship between energy balance and the metabolic profiles of plasma and milk.
32113768	3	57	theme	nuclear	460:466	arg1	resonance					477:485	nuclear magnetic resonance	460:485	nuclear magnetic resonance (NMR)	460:491	In this study our aims were: (1) to reveal the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS); and (2) to investigate the relationship between energy balance and the metabolic profiles of plasma and milk.
32113768	3	58	dep	reveal	383:388	arg1	1					377:377	1	377:377	1	377:377	In this study our aims were: (1) to reveal the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS); and (2) to investigate the relationship between energy balance and the metabolic profiles of plasma and milk.
32113768	3	58	dep	reveal	383:388	arg1	investigate					586:596	investigate	586:596	to investigate the relationship between energy balance and the metabolic profiles of plasma and milk	583:682	In this study our aims were: (1) to reveal the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS); and (2) to investigate the relationship between energy balance and the metabolic profiles of plasma and milk.
32113768	9	59	theme	body	1219:1222	arg1	mobilization					1232:1243	body reserve mobilization	1219:1243	body reserve mobilization from body fat, muscle, and bone	1219:1275	These plasma metabolites were related to body reserve mobilization from body fat, muscle, and bone; increased blood flow; and gluconeogenesis.
32113768	7	60	theme	milk	1038:1041	arg1	samples					1043:1049	milk samples	1038:1049	milk samples	1038:1049	Plasma and milk samples were collected and analyzed using LC-MS and NMR.
32113768	5	61	dep	kg/d	887:890	arg1	deviation					909:917	mean ± standard deviation	893:917	mean ± standard deviation	893:917	Body weight (mean ± standard deviation; 627.4 ± 56.4 kg) and milk yield (28.1 ± 6.7 kg/d; mean ± standard deviation) were monitored daily.
32113768	3	62	theme	milk	427:430	arg1	profiles					404:411	the metabolic profiles	390:411	the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS)	390:572	In this study our aims were: (1) to reveal the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS); and (2) to investigate the relationship between energy balance and the metabolic profiles of plasma and milk.
32113768	9	63	theme	plasma	1184:1189	arg1	metabolites					1191:1201	These plasma metabolites	1178:1201	These plasma metabolites	1178:1201	These plasma metabolites were related to body reserve mobilization from body fat, muscle, and bone; increased blood flow; and gluconeogenesis.
32113768	2	64	theme	metabolic	264:272	arg1	profile					274:280	metabolic profile	264:280	metabolic profile in plasma	264:290	However, the relationships among energy balance, metabolic profile in plasma, and metabolic profile in milk have not been reported.
32113768	4	65	theme	±	749:749	arg1	deviation					760:768	mean ± standard deviation	744:768	mean ± standard deviation	744:768	For this study 24 individual dairy cows (parity 2.5 ± 0.5; mean ± standard deviation) were studied in lactation wk 2.
32113768	9	66	theme	blood	1288:1292	arg1	flow					1294:1297	increased blood flow	1278:1297	increased blood flow	1278:1297	These plasma metabolites were related to body reserve mobilization from body fat, muscle, and bone; increased blood flow; and gluconeogenesis.
32113768	11	67	theme	cell	1436:1439	arg1	apoptosis					1441:1449	cell apoptosis	1436:1449	cell apoptosis	1436:1449	These milk metabolites were related to cell apoptosis and cell proliferation.
32113768	4	68	theme	parity	726:731	arg1	±					737:737	parity 2.5 ± 0.5	726:741	parity 2.5 ± 0.5	726:741	For this study 24 individual dairy cows (parity 2.5 ± 0.5; mean ± standard deviation) were studied in lactation wk 2.
32113768	4	68	theme	parity	726:731	arg1	cows					720:723	24 individual dairy cows	700:723	24 individual dairy cows (parity 2.5 ± 0.5; mean ± standard deviation)	700:769	For this study 24 individual dairy cows (parity 2.5 ± 0.5; mean ± standard deviation) were studied in lactation wk 2.
32113768	3	69	theme	energy	623:628	arg1	balance					630:636	energy balance	623:636	energy balance	623:636	In this study our aims were: (1) to reveal the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS); and (2) to investigate the relationship between energy balance and the metabolic profiles of plasma and milk.
32113768	9	70	theme	reserve	1224:1230	arg1	mobilization					1232:1243	body reserve mobilization	1219:1243	body reserve mobilization from body fat, muscle, and bone	1219:1275	These plasma metabolites were related to body reserve mobilization from body fat, muscle, and bone; increased blood flow; and gluconeogenesis.
32113768	9	71	from	muscle	1260:1265	arg1	gluconeogenesis					1304:1318	gluconeogenesis	1304:1318	gluconeogenesis	1304:1318	These plasma metabolites were related to body reserve mobilization from body fat, muscle, and bone; increased blood flow; and gluconeogenesis.
32113768	9	71	from	muscle	1260:1265	arg1	mobilization					1232:1243	body reserve mobilization	1219:1243	body reserve mobilization from body fat, muscle, and bone	1219:1275	These plasma metabolites were related to body reserve mobilization from body fat, muscle, and bone; increased blood flow; and gluconeogenesis.
32113768	9	71	from	muscle	1260:1265	arg1	flow					1294:1297	increased blood flow	1278:1297	increased blood flow	1278:1297	These plasma metabolites were related to body reserve mobilization from body fat, muscle, and bone; increased blood flow; and gluconeogenesis.
32113768	5	72	theme	milk	864:867	arg1	kg/d					887:890	28.1 ± 6.7 kg/d	876:890	28.1 ± 6.7 kg/d	876:890	Body weight (mean ± standard deviation; 627.4 ± 56.4 kg) and milk yield (28.1 ± 6.7 kg/d; mean ± standard deviation) were monitored daily.
32113768	5	72	theme	milk	864:867	arg1	yield					869:873	milk yield	864:873	milk yield (28.1 ± 6.7 kg/d; mean ± standard deviation)	864:918	Body weight (mean ± standard deviation; 627.4 ± 56.4 kg) and milk yield (28.1 ± 6.7 kg/d; mean ± standard deviation) were monitored daily.
32113768	6	73	theme	Milk	942:945	arg1	composition					947:957	Milk composition	942:957	Milk composition (fat, protein, and lactose)	942:985	Milk composition (fat, protein, and lactose) and net energy balance were calculated.
32113768	3	74	theme	metabolic	646:654	arg1	profiles					656:663	the metabolic profiles	642:663	the metabolic profiles of plasma and milk	642:682	In this study our aims were: (1) to reveal the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS); and (2) to investigate the relationship between energy balance and the metabolic profiles of plasma and milk.
32113768	3	75	theme	liquid	508:513	arg1	LC-MS					567:571	LC-MS	567:571	LC-MS	567:571	In this study our aims were: (1) to reveal the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS); and (2) to investigate the relationship between energy balance and the metabolic profiles of plasma and milk.
32113768	3	75	theme	liquid	508:513	arg1	spectrometry					553:564	liquid chromatography triple quadrupole mass spectrometry	508:564	liquid chromatography triple quadrupole mass spectrometry (LC-MS)	508:572	In this study our aims were: (1) to reveal the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS); and (2) to investigate the relationship between energy balance and the metabolic profiles of plasma and milk.
32113768	5	76	theme	mean	893:896	arg1	deviation					909:917	mean ± standard deviation	893:917	mean ± standard deviation	893:917	Body weight (mean ± standard deviation; 627.4 ± 56.4 kg) and milk yield (28.1 ± 6.7 kg/d; mean ± standard deviation) were monitored daily.
32113768	15	77	theme	new	2183:2185	arg1	indicators					2187:2196	new indicators	2183:2196	new indicators for energy balance	2183:2215	In addition to the classical indicators for energy balance (e.g., β-hydroxybutyrate, acetone, and glucose), the current study presents some new metabolites (e.g., glycine in plasma and milk; kynurenine, panthothenate, or arginine in plasma) in lactating dairy cows that are related to energy balance and may be of interest as new indicators for energy balance.
32113768	15	78	from	glycine	2020:2026	arg1	plasma					2031:2036	plasma	2031:2036	plasma	2031:2036	In addition to the classical indicators for energy balance (e.g., β-hydroxybutyrate, acetone, and glucose), the current study presents some new metabolites (e.g., glycine in plasma and milk; kynurenine, panthothenate, or arginine in plasma) in lactating dairy cows that are related to energy balance and may be of interest as new indicators for energy balance.
32113768	15	78	from	glycine	2020:2026	arg1	plasma					2090:2095	plasma	2090:2095	plasma	2090:2095	In addition to the classical indicators for energy balance (e.g., β-hydroxybutyrate, acetone, and glucose), the current study presents some new metabolites (e.g., glycine in plasma and milk; kynurenine, panthothenate, or arginine in plasma) in lactating dairy cows that are related to energy balance and may be of interest as new indicators for energy balance.
32113768	15	78	from	glycine	2020:2026	arg1	milk					2042:2045	milk	2042:2045	milk	2042:2045	In addition to the classical indicators for energy balance (e.g., β-hydroxybutyrate, acetone, and glucose), the current study presents some new metabolites (e.g., glycine in plasma and milk; kynurenine, panthothenate, or arginine in plasma) in lactating dairy cows that are related to energy balance and may be of interest as new indicators for energy balance.
32113768	6	79	theme	energy	995:1000	arg1	balance					1002:1008	net energy balance	991:1008	net energy balance	991:1008	Milk composition (fat, protein, and lactose) and net energy balance were calculated.
32113768	5	80	dep	deviation	832:840	arg1	kg					856:857	627.4 ± 56.4 kg	843:857	627.4 ± 56.4 kg	843:857	Body weight (mean ± standard deviation; 627.4 ± 56.4 kg) and milk yield (28.1 ± 6.7 kg/d; mean ± standard deviation) were monitored daily.
32113768	3	81	theme	mass	548:551	arg1	LC-MS					567:571	LC-MS	567:571	LC-MS	567:571	In this study our aims were: (1) to reveal the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS); and (2) to investigate the relationship between energy balance and the metabolic profiles of plasma and milk.
32113768	3	81	theme	mass	548:551	arg1	spectrometry					553:564	liquid chromatography triple quadrupole mass spectrometry	508:564	liquid chromatography triple quadrupole mass spectrometry (LC-MS)	508:572	In this study our aims were: (1) to reveal the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS); and (2) to investigate the relationship between energy balance and the metabolic profiles of plasma and milk.
32113768	1	82	theme	Negative	112:119	arg1	balance					128:134	Negative energy balance	112:134	Negative energy balance in dairy cows in early lactation	112:167	Negative energy balance in dairy cows in early lactation is related to alteration of metabolic status.
32113768	3	83	theme	triple	530:535	arg1	LC-MS					567:571	LC-MS	567:571	LC-MS	567:571	In this study our aims were: (1) to reveal the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS); and (2) to investigate the relationship between energy balance and the metabolic profiles of plasma and milk.
32113768	3	83	theme	triple	530:535	arg1	spectrometry					553:564	liquid chromatography triple quadrupole mass spectrometry	508:564	liquid chromatography triple quadrupole mass spectrometry (LC-MS)	508:572	In this study our aims were: (1) to reveal the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS); and (2) to investigate the relationship between energy balance and the metabolic profiles of plasma and milk.
32113768	15	84	theme	energy	2142:2147	arg1	balance					2149:2155	energy balance	2142:2155	energy balance	2142:2155	In addition to the classical indicators for energy balance (e.g., β-hydroxybutyrate, acetone, and glucose), the current study presents some new metabolites (e.g., glycine in plasma and milk; kynurenine, panthothenate, or arginine in plasma) in lactating dairy cows that are related to energy balance and may be of interest as new indicators for energy balance.
32113768	1	85	theme	dairy	139:143	arg1	cows					145:148	dairy cows	139:148	dairy cows	139:148	Negative energy balance in dairy cows in early lactation is related to alteration of metabolic status.
32113768	2	86	from	profile	274:280	arg1	plasma					285:290	plasma	285:290	plasma	285:290	However, the relationships among energy balance, metabolic profile in plasma, and metabolic profile in milk have not been reported.
32113768	2	86	from	profile	274:280	arg1	milk					318:321	milk	318:321	milk	318:321	However, the relationships among energy balance, metabolic profile in plasma, and metabolic profile in milk have not been reported.
32113768	15	87	from	arginine	2078:2085	arg1	plasma					2031:2036	plasma	2031:2036	plasma	2031:2036	In addition to the classical indicators for energy balance (e.g., β-hydroxybutyrate, acetone, and glucose), the current study presents some new metabolites (e.g., glycine in plasma and milk; kynurenine, panthothenate, or arginine in plasma) in lactating dairy cows that are related to energy balance and may be of interest as new indicators for energy balance.
32113768	15	87	from	arginine	2078:2085	arg1	plasma					2090:2095	plasma	2090:2095	plasma	2090:2095	In addition to the classical indicators for energy balance (e.g., β-hydroxybutyrate, acetone, and glucose), the current study presents some new metabolites (e.g., glycine in plasma and milk; kynurenine, panthothenate, or arginine in plasma) in lactating dairy cows that are related to energy balance and may be of interest as new indicators for energy balance.
32113768	15	87	from	arginine	2078:2085	arg1	milk					2042:2045	milk	2042:2045	milk	2042:2045	In addition to the classical indicators for energy balance (e.g., β-hydroxybutyrate, acetone, and glucose), the current study presents some new metabolites (e.g., glycine in plasma and milk; kynurenine, panthothenate, or arginine in plasma) in lactating dairy cows that are related to energy balance and may be of interest as new indicators for energy balance.
32113768	13	88	theme	acids	1660:1664	arg1	hyperketonemia					1623:1636	hyperketonemia	1623:1636	hyperketonemia	1623:1636	These metabolites were mainly related to hyperketonemia; β-oxidation of fatty acids; and one-carbon metabolism.
32113768	13	88	theme	acids	1660:1664	arg1	metabolism					1682:1691	one-carbon metabolism	1671:1691	one-carbon metabolism	1671:1691	These metabolites were mainly related to hyperketonemia; β-oxidation of fatty acids; and one-carbon metabolism.
32113768	13	88	theme	acids	1660:1664	arg1	β-oxidation					1639:1649	β-oxidation	1639:1649	β-oxidation of fatty acids	1639:1664	These metabolites were mainly related to hyperketonemia; β-oxidation of fatty acids; and one-carbon metabolism.
32113768	0	89	theme	cows	87:90	arg1	milk					73:76	milk	73:76	milk	73:76	Relationship between energy balance and metabolic profiles in plasma and milk of dairy cows in early lactation.
32113768	0	89	theme	cows	87:90	arg1	plasma					62:67	plasma	62:67	plasma	62:67	Relationship between energy balance and metabolic profiles in plasma and milk of dairy cows in early lactation.
32113768	5	90	theme	mean	816:819	arg1	weight					808:813	Body weight	803:813	Body weight (mean ± standard deviation; 627.4 ± 56.4 kg)	803:858	Body weight (mean ± standard deviation; 627.4 ± 56.4 kg) and milk yield (28.1 ± 6.7 kg/d; mean ± standard deviation) were monitored daily.
32113768	5	90	theme	mean	816:819	arg1	deviation					832:840	mean ± standard deviation	816:840	mean ± standard deviation	816:840	Body weight (mean ± standard deviation; 627.4 ± 56.4 kg) and milk yield (28.1 ± 6.7 kg/d; mean ± standard deviation) were monitored daily.
32113768	8	91	theme	plasma	1109:1114	arg1	metabolites					1116:1126	all plasma metabolites	1105:1126	all plasma metabolites measured	1105:1135	From all plasma metabolites measured, 27 were correlated with energy balance.
32113768	0	92	theme	early	95:99	arg1	lactation					101:109	early lactation	95:109	early lactation	95:109	Relationship between energy balance and metabolic profiles in plasma and milk of dairy cows in early lactation.
32113768	5	93	theme	standard	900:907	arg1	deviation					909:917	mean ± standard deviation	893:917	mean ± standard deviation	893:917	Body weight (mean ± standard deviation; 627.4 ± 56.4 kg) and milk yield (28.1 ± 6.7 kg/d; mean ± standard deviation) were monitored daily.
32113768	14	94	from	pathways	1787:1794	arg1	balance					1829:1835	negative energy balance	1813:1835	negative energy balance in early lactation	1813:1854	The metabolic profiles of plasma and milk provide an in-depth insight into the physiological pathways of dairy cows in negative energy balance in early lactation.
32113768	5	95	theme	standard	823:830	arg1	weight					808:813	Body weight	803:813	Body weight (mean ± standard deviation; 627.4 ± 56.4 kg)	803:858	Body weight (mean ± standard deviation; 627.4 ± 56.4 kg) and milk yield (28.1 ± 6.7 kg/d; mean ± standard deviation) were monitored daily.
32113768	5	95	theme	standard	823:830	arg1	deviation					832:840	mean ± standard deviation	816:840	mean ± standard deviation	816:840	Body weight (mean ± standard deviation; 627.4 ± 56.4 kg) and milk yield (28.1 ± 6.7 kg/d; mean ± standard deviation) were monitored daily.
32113768	15	96	theme	dairy	2111:2115	arg1	cows					2117:2120	lactating dairy cows	2101:2120	lactating dairy cows	2101:2120	In addition to the classical indicators for energy balance (e.g., β-hydroxybutyrate, acetone, and glucose), the current study presents some new metabolites (e.g., glycine in plasma and milk; kynurenine, panthothenate, or arginine in plasma) in lactating dairy cows that are related to energy balance and may be of interest as new indicators for energy balance.
32113768	14	97	theme	metabolic	1698:1706	arg1	profiles					1708:1715	The metabolic profiles	1694:1715	The metabolic profiles of plasma and milk	1694:1734	The metabolic profiles of plasma and milk provide an in-depth insight into the physiological pathways of dairy cows in negative energy balance in early lactation.
32113768	14	98	theme	in-depth	1747:1754	arg1	insight					1756:1762	an in-depth insight	1744:1762	an in-depth insight into the physiological pathways of dairy cows in negative energy balance in early lactation	1744:1854	The metabolic profiles of plasma and milk provide an in-depth insight into the physiological pathways of dairy cows in negative energy balance in early lactation.
32113768	14	99	theme	physiological	1773:1785	arg1	pathways					1787:1794	the physiological pathways	1769:1794	the physiological pathways of dairy cows in negative energy balance in early lactation	1769:1854	The metabolic profiles of plasma and milk provide an in-depth insight into the physiological pathways of dairy cows in negative energy balance in early lactation.
32113768	11	100	theme	cell	1455:1458	arg1	proliferation					1460:1472	cell proliferation	1455:1472	cell proliferation	1455:1472	These milk metabolites were related to cell apoptosis and cell proliferation.
32113768	3	101	theme	metabolic	394:402	arg1	profiles					404:411	the metabolic profiles	390:411	the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS)	390:572	In this study our aims were: (1) to reveal the metabolic profiles of plasma and milk by integrating results from nuclear magnetic resonance (NMR) with data from liquid chromatography triple quadrupole mass spectrometry (LC-MS); and (2) to investigate the relationship between energy balance and the metabolic profiles of plasma and milk.
32113768	0	102	theme	metabolic	40:48	arg1	profiles					50:57	metabolic profiles	40:57	metabolic profiles	40:57	Relationship between energy balance and metabolic profiles in plasma and milk of dairy cows in early lactation.
32113768	15	103	theme	classical	1876:1884	arg1	indicators					1886:1895	the classical indicators	1872:1895	the classical indicators for energy balance (e.g., β-hydroxybutyrate, acetone, and glucose)	1872:1962	In addition to the classical indicators for energy balance (e.g., β-hydroxybutyrate, acetone, and glucose), the current study presents some new metabolites (e.g., glycine in plasma and milk; kynurenine, panthothenate, or arginine in plasma) in lactating dairy cows that are related to energy balance and may be of interest as new indicators for energy balance.
32113768	14	104	theme	energy	1822:1827	arg1	balance					1829:1835	negative energy balance	1813:1835	negative energy balance in early lactation	1813:1854	The metabolic profiles of plasma and milk provide an in-depth insight into the physiological pathways of dairy cows in negative energy balance in early lactation.
32113768	8	105	theme	energy	1162:1167	arg1	balance					1169:1175	energy balance	1162:1175	energy balance	1162:1175	From all plasma metabolites measured, 27 were correlated with energy balance.
32113768	15	106	theme	new	1997:1999	arg1	metabolites					2001:2011	some new metabolites	1992:2011	some new metabolites (e.g., glycine in plasma and milk; kynurenine, panthothenate, or arginine in plasma) in lactating dairy cows that are related to energy balance and may be of interest as new indicators for energy balance	1992:2215	In addition to the classical indicators for energy balance (e.g., β-hydroxybutyrate, acetone, and glucose), the current study presents some new metabolites (e.g., glycine in plasma and milk; kynurenine, panthothenate, or arginine in plasma) in lactating dairy cows that are related to energy balance and may be of interest as new indicators for energy balance.
32113768	12	107	theme	energy	1566:1571	arg1	balance					1573:1579	energy balance	1566:1579	energy balance	1566:1579	Nine metabolites detected in both plasma and milk were correlated with each other and with energy balance.
32113768	14	108	theme	dairy	1799:1803	arg1	cows					1805:1808	dairy cows	1799:1808	dairy cows	1799:1808	The metabolic profiles of plasma and milk provide an in-depth insight into the physiological pathways of dairy cows in negative energy balance in early lactation.
32113768	1	109	theme	status	207:212	arg1	alteration					183:192	alteration	183:192	alteration of metabolic status	183:212	Negative energy balance in dairy cows in early lactation is related to alteration of metabolic status.
32113768	5	110	theme	±	849:849	arg1	kg					856:857	627.4 ± 56.4 kg	843:857	627.4 ± 56.4 kg	843:857	Body weight (mean ± standard deviation; 627.4 ± 56.4 kg) and milk yield (28.1 ± 6.7 kg/d; mean ± standard deviation) were monitored daily.
32113768	11	111	theme	milk	1403:1406	arg1	metabolites					1408:1418	These milk metabolites	1397:1418	These milk metabolites	1397:1418	These milk metabolites were related to cell apoptosis and cell proliferation.
32024873	5	0	theme	cartilage	685:693	arg1	times					712:716	(mean ± SEM) patellar, tibial, and femoral cartilage T1rho relaxation times	642:716	(mean ± SEM) patellar, tibial, and femoral cartilage T1rho relaxation times	642:716	Overall, (mean ± SEM) patellar, tibial, and femoral cartilage T1rho relaxation times significantly decreased immediately after running 3 (65 ± 3 ms to 62 ± 3 ms; p = 0.04) and 10 (69 ± 4 ms to 62 ± 3 ms; p < 0.001) miles.
32024873	1	1	theme	run	140:142	arg1	%					125:125	Roughly 20%	115:125	Roughly 20% of Americans run	115:142	Roughly 20% of Americans run annually, yet how this exercise influences knee cartilage health is poorly understood.
32024873	1	1	theme	run	140:142	arg1	run					140:142	Americans run	130:142	Americans run	130:142	Roughly 20% of Americans run annually, yet how this exercise influences knee cartilage health is poorly understood.
32024873	4	2	theme	asymptomatic	536:547	arg1	runners					555:561	eight asymptomatic, male runners	530:561	eight asymptomatic, male runners before, immediately after	530:587	In this study, T1rho MRI was performed on the dominant knee of eight asymptomatic, male runners before, immediately after, and 24 hours after running 3 and 10 miles.
32024873	5	3	theme	T1rho	695:699	arg1	times					712:716	(mean ± SEM) patellar, tibial, and femoral cartilage T1rho relaxation times	642:716	(mean ± SEM) patellar, tibial, and femoral cartilage T1rho relaxation times	642:716	Overall, (mean ± SEM) patellar, tibial, and femoral cartilage T1rho relaxation times significantly decreased immediately after running 3 (65 ± 3 ms to 62 ± 3 ms; p = 0.04) and 10 (69 ± 4 ms to 62 ± 3 ms; p < 0.001) miles.
32024873	4	4	theme	T1rho	482:486	arg1	MRI					488:490	T1rho MRI	482:490	T1rho MRI	482:490	In this study, T1rho MRI was performed on the dominant knee of eight asymptomatic, male runners before, immediately after, and 24 hours after running 3 and 10 miles.
32024873	5	5	dep	3	768:768	arg1	65 ± 3 ms					771:779	65 ± 3 ms	771:779	65 ± 3 ms to 62 ± 3 ms; p = 0.04	771:802	Overall, (mean ± SEM) patellar, tibial, and femoral cartilage T1rho relaxation times significantly decreased immediately after running 3 (65 ± 3 ms to 62 ± 3 ms; p = 0.04) and 10 (69 ± 4 ms to 62 ± 3 ms; p < 0.001) miles.
32024873	9	6	theme	asymptomatic	1393:1404	arg1	runners					1411:1417	asymptomatic male runners	1393:1417	asymptomatic male runners	1393:1417	This information may inform safe exercise and recovery protocols in asymptomatic male runners by characterizing running-induced changes in knee cartilage composition.
32024873	0	7	theme	magnetic	87:94	arg1	imaging					106:112	T1rho magnetic resonance imaging	81:112	T1rho magnetic resonance imaging	81:112	Quantifying the biochemical state of knee cartilage in response to running using T1rho magnetic resonance imaging.
32024873	7	8	theme	Percent	1001:1007	arg1	decreases					1009:1017	Percent decreases	1001:1017	Percent decreases in T1rho relaxation times	1001:1043	Percent decreases in T1rho relaxation times were significantly larger following 10 mile runs as compared to 3 mile runs (11 ± 1% vs. 4 ± 1%; p = 0.02).
32024873	7	9	dep	%	1128:1128	arg1	p = 0.02					1142:1149	p = 0.02	1142:1149	p = 0.02	1142:1149	Percent decreases in T1rho relaxation times were significantly larger following 10 mile runs as compared to 3 mile runs (11 ± 1% vs. 4 ± 1%; p = 0.02).
32024873	2	10	theme	magnetic	270:277	arg1	imaging					289:295	quantitative magnetic resonance imaging	257:295	quantitative magnetic resonance imaging (MRI)	257:301	To address this question, quantitative magnetic resonance imaging (MRI) can be used to infer the biochemical state of cartilage.
32024873	2	10	theme	magnetic	270:277	arg1	MRI					298:300	MRI	298:300	MRI	298:300	To address this question, quantitative magnetic resonance imaging (MRI) can be used to infer the biochemical state of cartilage.
32024873	9	11	theme	knee	1464:1467	arg1	composition					1479:1489	knee cartilage composition	1464:1489	knee cartilage composition	1464:1489	This information may inform safe exercise and recovery protocols in asymptomatic male runners by characterizing running-induced changes in knee cartilage composition.
32024873	6	12	theme	T1rho	916:920	arg1	values					922:927	pre-exercise and recovery T1rho values	890:927	pre-exercise and recovery T1rho values	890:927	No significant differences between pre-exercise and recovery T1rho values were observed for either distance (3 mile: p = 0.8; 10 mile: p = 0.08).
32024873	0	13	theme	T1rho	81:85	arg1	imaging					106:112	T1rho magnetic resonance imaging	81:112	T1rho magnetic resonance imaging	81:112	Quantifying the biochemical state of knee cartilage in response to running using T1rho magnetic resonance imaging.
32024873	8	14	theme	relative	1196:1203	arg1	concentration					1218:1230	the relative proteoglycan concentration	1192:1230	the relative proteoglycan concentration of knee cartilage due to water flow	1192:1266	This data suggests that alterations to the relative proteoglycan concentration of knee cartilage due to water flow are mitigated within 24 hours of running up to 10 miles.
32024873	2	15	theme	quantitative	257:268	arg1	imaging					289:295	quantitative magnetic resonance imaging	257:295	quantitative magnetic resonance imaging (MRI)	257:301	To address this question, quantitative magnetic resonance imaging (MRI) can be used to infer the biochemical state of cartilage.
32024873	2	15	theme	quantitative	257:268	arg1	MRI					298:300	MRI	298:300	MRI	298:300	To address this question, quantitative magnetic resonance imaging (MRI) can be used to infer the biochemical state of cartilage.
32024873	6	16	theme	recovery	907:914	arg1	values					922:927	pre-exercise and recovery T1rho values	890:927	pre-exercise and recovery T1rho values	890:927	No significant differences between pre-exercise and recovery T1rho values were observed for either distance (3 mile: p = 0.8; 10 mile: p = 0.08).
32024873	5	17	theme	tibial	665:670	arg1	times					712:716	(mean ± SEM) patellar, tibial, and femoral cartilage T1rho relaxation times	642:716	(mean ± SEM) patellar, tibial, and femoral cartilage T1rho relaxation times	642:716	Overall, (mean ± SEM) patellar, tibial, and femoral cartilage T1rho relaxation times significantly decreased immediately after running 3 (65 ± 3 ms to 62 ± 3 ms; p = 0.04) and 10 (69 ± 4 ms to 62 ± 3 ms; p < 0.001) miles.
32024873	9	18	from	protocols	1380:1388	arg1	runners					1411:1417	asymptomatic male runners	1393:1417	asymptomatic male runners	1393:1417	This information may inform safe exercise and recovery protocols in asymptomatic male runners by characterizing running-induced changes in knee cartilage composition.
32024873	0	19	theme	resonance	96:104	arg1	imaging					106:112	T1rho magnetic resonance imaging	81:112	T1rho magnetic resonance imaging	81:112	Quantifying the biochemical state of knee cartilage in response to running using T1rho magnetic resonance imaging.
32024873	6	20	dep	distance	954:961	arg1	mile					966:969	3 mile	964:969	3 mile: p = 0.8; 10 mile: p = 0.08	964:997	No significant differences between pre-exercise and recovery T1rho values were observed for either distance (3 mile: p = 0.8; 10 mile: p = 0.08).
32024873	0	21	theme	knee	37:40	arg1	cartilage					42:50	knee cartilage	37:50	knee cartilage	37:50	Quantifying the biochemical state of knee cartilage in response to running using T1rho magnetic resonance imaging.
32024873	9	22	from	exercise	1358:1365	arg1	runners					1411:1417	asymptomatic male runners	1393:1417	asymptomatic male runners	1393:1417	This information may inform safe exercise and recovery protocols in asymptomatic male runners by characterizing running-induced changes in knee cartilage composition.
32024873	6	23	dep	mile	966:969	arg1	p = 0.08					990:997	p = 0.08	990:997	p = 0.08	990:997	No significant differences between pre-exercise and recovery T1rho values were observed for either distance (3 mile: p = 0.8; 10 mile: p = 0.08).
32024873	6	23	dep	mile	966:969	arg1	p = 0.8					972:978	p = 0.8	972:978	p = 0.8	972:978	No significant differences between pre-exercise and recovery T1rho values were observed for either distance (3 mile: p = 0.8; 10 mile: p = 0.08).
32024873	6	23	dep	mile	966:969	arg1	mile					984:987	10 mile	981:987	3 mile: p = 0.8; 10 mile: p = 0.08	964:997	No significant differences between pre-exercise and recovery T1rho values were observed for either distance (3 mile: p = 0.8; 10 mile: p = 0.08).
32024873	2	24	theme	cartilage	349:357	arg1	state					340:344	the biochemical state	324:344	the biochemical state of cartilage	324:357	To address this question, quantitative magnetic resonance imaging (MRI) can be used to infer the biochemical state of cartilage.
32024873	8	25	dep	10	1315:1316	arg1	to					1312:1313	to	1312:1313	to	1312:1313	This data suggests that alterations to the relative proteoglycan concentration of knee cartilage due to water flow are mitigated within 24 hours of running up to 10 miles.
32024873	7	26	theme	mile	1111:1114	arg1	runs					1116:1119	3 mile runs	1109:1119	3 mile runs (11 ± 1% vs. 4 ± 1%; p = 0.02)	1109:1150	Percent decreases in T1rho relaxation times were significantly larger following 10 mile runs as compared to 3 mile runs (11 ± 1% vs. 4 ± 1%; p = 0.02).
32024873	7	26	theme	mile	1111:1114	arg1	%					1128:1128	11 ± 1%	1122:1128	11 ± 1%	1122:1128	Percent decreases in T1rho relaxation times were significantly larger following 10 mile runs as compared to 3 mile runs (11 ± 1% vs. 4 ± 1%; p = 0.02).
32024873	7	26	theme	mile	1111:1114	arg1	%					1139:1139	4 ± 1%	1134:1139	4 ± 1%	1134:1139	Percent decreases in T1rho relaxation times were significantly larger following 10 mile runs as compared to 3 mile runs (11 ± 1% vs. 4 ± 1%; p = 0.02).
32024873	5	27	dep	69 ± 4 ms	813:821	arg1	p < 0.001					837:845	p < 0.001	837:845	p < 0.001	837:845	Overall, (mean ± SEM) patellar, tibial, and femoral cartilage T1rho relaxation times significantly decreased immediately after running 3 (65 ± 3 ms to 62 ± 3 ms; p = 0.04) and 10 (69 ± 4 ms to 62 ± 3 ms; p < 0.001) miles.
32024873	4	28	theme	24 hours	594:601	arg1	knee					522:525	the dominant knee	509:525	the dominant knee of eight asymptomatic, male runners before, immediately after, and 24 hours	509:601	In this study, T1rho MRI was performed on the dominant knee of eight asymptomatic, male runners before, immediately after, and 24 hours after running 3 and 10 miles.
32024873	7	29	theme	mile	1084:1087	arg1	runs					1089:1092	10 mile runs	1081:1092	10 mile runs	1081:1092	Percent decreases in T1rho relaxation times were significantly larger following 10 mile runs as compared to 3 mile runs (11 ± 1% vs. 4 ± 1%; p = 0.02).
32024873	4	30	theme	dominant	513:520	arg1	knee					522:525	the dominant knee	509:525	the dominant knee of eight asymptomatic, male runners before, immediately after, and 24 hours	509:601	In this study, T1rho MRI was performed on the dominant knee of eight asymptomatic, male runners before, immediately after, and 24 hours after running 3 and 10 miles.
32024873	0	31	dep	running	67:73	arg1	response					55:62	response	55:62	response	55:62	Quantifying the biochemical state of knee cartilage in response to running using T1rho magnetic resonance imaging.
32024873	7	32	theme	T1rho	1022:1026	arg1	times					1039:1043	T1rho relaxation times	1022:1043	T1rho relaxation times	1022:1043	Percent decreases in T1rho relaxation times were significantly larger following 10 mile runs as compared to 3 mile runs (11 ± 1% vs. 4 ± 1%; p = 0.02).
32024873	6	33	theme	pre-exercise	890:901	arg1	values					922:927	pre-exercise and recovery T1rho values	890:927	pre-exercise and recovery T1rho values	890:927	No significant differences between pre-exercise and recovery T1rho values were observed for either distance (3 mile: p = 0.8; 10 mile: p = 0.08).
32024873	2	34	used	used	310:313	arg2	MRI					298:300	MRI	298:300	MRI	298:300	To address this question, quantitative magnetic resonance imaging (MRI) can be used to infer the biochemical state of cartilage.
32024873	2	34	used	used	310:313	arg2	imaging					289:295	quantitative magnetic resonance imaging	257:295	quantitative magnetic resonance imaging (MRI)	257:301	To address this question, quantitative magnetic resonance imaging (MRI) can be used to infer the biochemical state of cartilage.
32024873	3	35	theme	proteoglycan	426:437	arg1	concentration					439:451	the proteoglycan concentration	422:451	the proteoglycan concentration in cartilage	422:464	Specifically, T1rho relaxation times are inversely related to the proteoglycan concentration in cartilage.
32024873	0	36	theme	biochemical	16:26	arg1	state					28:32	the biochemical state	12:32	the biochemical state of knee cartilage	12:50	Quantifying the biochemical state of knee cartilage in response to running using T1rho magnetic resonance imaging.
32024873	2	37	theme	resonance	279:287	arg1	imaging					289:295	quantitative magnetic resonance imaging	257:295	quantitative magnetic resonance imaging (MRI)	257:301	To address this question, quantitative magnetic resonance imaging (MRI) can be used to infer the biochemical state of cartilage.
32024873	2	37	theme	resonance	279:287	arg1	MRI					298:300	MRI	298:300	MRI	298:300	To address this question, quantitative magnetic resonance imaging (MRI) can be used to infer the biochemical state of cartilage.
32024873	5	38	theme	10	809:810	arg1	miles					848:852	3 (65 ± 3 ms to 62 ± 3 ms; p = 0.04) and 10 (69 ± 4 ms to 62 ± 3 ms; p < 0.001) miles	768:852	3 (65 ± 3 ms to 62 ± 3 ms; p = 0.04) and 10 (69 ± 4 ms to 62 ± 3 ms; p < 0.001) miles	768:852	Overall, (mean ± SEM) patellar, tibial, and femoral cartilage T1rho relaxation times significantly decreased immediately after running 3 (65 ± 3 ms to 62 ± 3 ms; p = 0.04) and 10 (69 ± 4 ms to 62 ± 3 ms; p < 0.001) miles.
32024873	3	39	theme	T1rho	374:378	arg1	times					391:395	T1rho relaxation times	374:395	T1rho relaxation times	374:395	Specifically, T1rho relaxation times are inversely related to the proteoglycan concentration in cartilage.
32024873	9	40	theme	running-induced	1437:1451	arg1	changes					1453:1459	running-induced changes	1437:1459	running-induced changes in knee cartilage composition	1437:1489	This information may inform safe exercise and recovery protocols in asymptomatic male runners by characterizing running-induced changes in knee cartilage composition.
32024873	1	41	dep	understood	219:228	arg1	influences					176:185	influences	176:185	influences knee cartilage health	176:207	Roughly 20% of Americans run annually, yet how this exercise influences knee cartilage health is poorly understood.
32024873	9	42	theme	cartilage	1469:1477	arg1	composition					1479:1489	knee cartilage composition	1464:1489	knee cartilage composition	1464:1489	This information may inform safe exercise and recovery protocols in asymptomatic male runners by characterizing running-induced changes in knee cartilage composition.
32024873	1	43	theme	knee	187:190	arg1	health					202:207	knee cartilage health	187:207	knee cartilage health	187:207	Roughly 20% of Americans run annually, yet how this exercise influences knee cartilage health is poorly understood.
32024873	3	44	theme	relaxation	380:389	arg1	times					391:395	T1rho relaxation times	374:395	T1rho relaxation times	374:395	Specifically, T1rho relaxation times are inversely related to the proteoglycan concentration in cartilage.
32024873	6	45	theme	significant	858:868	arg1	differences					870:880	No significant differences	855:880	No significant differences between pre-exercise and recovery T1rho values	855:927	No significant differences between pre-exercise and recovery T1rho values were observed for either distance (3 mile: p = 0.8; 10 mile: p = 0.08).
32024873	4	46	dep	asymptomatic	536:547	arg1	male					550:553	male	550:553	male	550:553	In this study, T1rho MRI was performed on the dominant knee of eight asymptomatic, male runners before, immediately after, and 24 hours after running 3 and 10 miles.
32024873	8	47	theme	water	1257:1261	arg1	flow					1263:1266	water flow	1257:1266	water flow	1257:1266	This data suggests that alterations to the relative proteoglycan concentration of knee cartilage due to water flow are mitigated within 24 hours of running up to 10 miles.
32024873	1	48	theme	cartilage	192:200	arg1	health					202:207	knee cartilage health	187:207	knee cartilage health	187:207	Roughly 20% of Americans run annually, yet how this exercise influences knee cartilage health is poorly understood.
32024873	7	49	theme	relaxation	1028:1037	arg1	times					1039:1043	T1rho relaxation times	1022:1043	T1rho relaxation times	1022:1043	Percent decreases in T1rho relaxation times were significantly larger following 10 mile runs as compared to 3 mile runs (11 ± 1% vs. 4 ± 1%; p = 0.02).
32024873	5	50	theme	patellar	655:662	arg1	times					712:716	(mean ± SEM) patellar, tibial, and femoral cartilage T1rho relaxation times	642:716	(mean ± SEM) patellar, tibial, and femoral cartilage T1rho relaxation times	642:716	Overall, (mean ± SEM) patellar, tibial, and femoral cartilage T1rho relaxation times significantly decreased immediately after running 3 (65 ± 3 ms to 62 ± 3 ms; p = 0.04) and 10 (69 ± 4 ms to 62 ± 3 ms; p < 0.001) miles.
32024873	8	51	theme	cartilage	1240:1248	arg1	concentration					1218:1230	the relative proteoglycan concentration	1192:1230	the relative proteoglycan concentration of knee cartilage due to water flow	1192:1266	This data suggests that alterations to the relative proteoglycan concentration of knee cartilage due to water flow are mitigated within 24 hours of running up to 10 miles.
32024873	8	52	theme	knee	1235:1238	arg1	cartilage					1240:1248	knee cartilage	1235:1248	knee cartilage	1235:1248	This data suggests that alterations to the relative proteoglycan concentration of knee cartilage due to water flow are mitigated within 24 hours of running up to 10 miles.
32024873	0	53	theme	cartilage	42:50	arg1	state					28:32	the biochemical state	12:32	the biochemical state of knee cartilage	12:50	Quantifying the biochemical state of knee cartilage in response to running using T1rho magnetic resonance imaging.
32024873	9	54	theme	male	1406:1409	arg1	runners					1411:1417	asymptomatic male runners	1393:1417	asymptomatic male runners	1393:1417	This information may inform safe exercise and recovery protocols in asymptomatic male runners by characterizing running-induced changes in knee cartilage composition.
32024873	9	55	theme	safe	1353:1356	arg1	exercise					1358:1365	safe exercise and recovery protocols	1353:1388	exercise	1358:1365	This information may inform safe exercise and recovery protocols in asymptomatic male runners by characterizing running-induced changes in knee cartilage composition.
32024873	5	56	dep	65 ± 3 ms	771:779	arg1	p = 0.04					795:802	p = 0.04	795:802	p = 0.04	795:802	Overall, (mean ± SEM) patellar, tibial, and femoral cartilage T1rho relaxation times significantly decreased immediately after running 3 (65 ± 3 ms to 62 ± 3 ms; p = 0.04) and 10 (69 ± 4 ms to 62 ± 3 ms; p < 0.001) miles.
32024873	3	57	from	concentration	439:451	arg1	cartilage					456:464	cartilage	456:464	cartilage	456:464	Specifically, T1rho relaxation times are inversely related to the proteoglycan concentration in cartilage.
32024873	5	58	theme	relaxation	701:710	arg1	times					712:716	(mean ± SEM) patellar, tibial, and femoral cartilage T1rho relaxation times	642:716	(mean ± SEM) patellar, tibial, and femoral cartilage T1rho relaxation times	642:716	Overall, (mean ± SEM) patellar, tibial, and femoral cartilage T1rho relaxation times significantly decreased immediately after running 3 (65 ± 3 ms to 62 ± 3 ms; p = 0.04) and 10 (69 ± 4 ms to 62 ± 3 ms; p < 0.001) miles.
32024873	8	59	theme	proteoglycan	1205:1216	arg1	concentration					1218:1230	the relative proteoglycan concentration	1192:1230	the relative proteoglycan concentration of knee cartilage due to water flow	1192:1266	This data suggests that alterations to the relative proteoglycan concentration of knee cartilage due to water flow are mitigated within 24 hours of running up to 10 miles.
32024873	4	60	theme	runners	555:561	arg1	knee					522:525	the dominant knee	509:525	the dominant knee of eight asymptomatic, male runners before, immediately after, and 24 hours	509:601	In this study, T1rho MRI was performed on the dominant knee of eight asymptomatic, male runners before, immediately after, and 24 hours after running 3 and 10 miles.
32024873	4	61	dep	runners	555:561	arg1	before					563:568	before	563:568	before	563:568	In this study, T1rho MRI was performed on the dominant knee of eight asymptomatic, male runners before, immediately after, and 24 hours after running 3 and 10 miles.
32024873	9	62	theme	recovery	1371:1378	arg1	protocols					1380:1388	safe exercise and recovery protocols	1353:1388	protocols	1380:1388	This information may inform safe exercise and recovery protocols in asymptomatic male runners by characterizing running-induced changes in knee cartilage composition.
32024873	2	63	theme	biochemical	328:338	arg1	state					340:344	the biochemical state	324:344	the biochemical state of cartilage	324:357	To address this question, quantitative magnetic resonance imaging (MRI) can be used to infer the biochemical state of cartilage.
32024873	9	64	from	changes	1453:1459	arg1	composition					1479:1489	knee cartilage composition	1464:1489	knee cartilage composition	1464:1489	This information may inform safe exercise and recovery protocols in asymptomatic male runners by characterizing running-induced changes in knee cartilage composition.
32024873	5	65	theme	femoral	677:683	arg1	times					712:716	(mean ± SEM) patellar, tibial, and femoral cartilage T1rho relaxation times	642:716	(mean ± SEM) patellar, tibial, and femoral cartilage T1rho relaxation times	642:716	Overall, (mean ± SEM) patellar, tibial, and femoral cartilage T1rho relaxation times significantly decreased immediately after running 3 (65 ± 3 ms to 62 ± 3 ms; p = 0.04) and 10 (69 ± 4 ms to 62 ± 3 ms; p < 0.001) miles.
32024873	5	66	theme	3	768:768	arg1	miles					848:852	3 (65 ± 3 ms to 62 ± 3 ms; p = 0.04) and 10 (69 ± 4 ms to 62 ± 3 ms; p < 0.001) miles	768:852	3 (65 ± 3 ms to 62 ± 3 ms; p = 0.04) and 10 (69 ± 4 ms to 62 ± 3 ms; p < 0.001) miles	768:852	Overall, (mean ± SEM) patellar, tibial, and femoral cartilage T1rho relaxation times significantly decreased immediately after running 3 (65 ± 3 ms to 62 ± 3 ms; p = 0.04) and 10 (69 ± 4 ms to 62 ± 3 ms; p < 0.001) miles.
32024873	1	67	theme	Americans	130:138	arg1	run					140:142	Americans run	130:142	Americans run	130:142	Roughly 20% of Americans run annually, yet how this exercise influences knee cartilage health is poorly understood.
32024873	7	68	from	decreases	1009:1017	arg1	times					1039:1043	T1rho relaxation times	1022:1043	T1rho relaxation times	1022:1043	Percent decreases in T1rho relaxation times were significantly larger following 10 mile runs as compared to 3 mile runs (11 ± 1% vs. 4 ± 1%; p = 0.02).
31675290	6	0	theme	Gryllotalpicola	787:801	arg1	PU-02T					814:819	Gryllotalpicola kribbensis PU-02T	787:819	Gryllotalpicola kribbensis PU-02T (97.5 %)	787:828	The 16S rRNA gene sequence of strain 2DFW10M-5T showed the highest similarity to Gryllotalpicola daejeonensis RU-04T (98.4 %), Gryllotalpicola soli KIS12-7T (98.2 %), Gryllotalpicola kribbensis PU-02T (97.5 %), Gryllotalpicola koreensis RU-16T (97.4 %) and Gryllotalpicola reticulitermitis TS-56T (97.2 %).
31675290	6	0	theme	Gryllotalpicola	787:801	arg1	%					827:827	97.5 %	822:827	97.5 %	822:827	The 16S rRNA gene sequence of strain 2DFW10M-5T showed the highest similarity to Gryllotalpicola daejeonensis RU-04T (98.4 %), Gryllotalpicola soli KIS12-7T (98.2 %), Gryllotalpicola kribbensis PU-02T (97.5 %), Gryllotalpicola koreensis RU-16T (97.4 %) and Gryllotalpicola reticulitermitis TS-56T (97.2 %).
31675290	11	1	with	glycine	1417:1423	arg1	l-lysine					1453:1460	l-lysine	1453:1460	l-lysine	1453:1460	The peptidoglycan contained d- and l-alanine, d-glutamic acid, glycine, l-serine and d-lysine with l-lysine as the diamino acid.
31675290	6	2	theme	daejeonensis	717:728	arg1	RU-04T					730:735	Gryllotalpicola daejeonensis RU-04T	701:735	Gryllotalpicola daejeonensis RU-04T (98.4 %)	701:744	The 16S rRNA gene sequence of strain 2DFW10M-5T showed the highest similarity to Gryllotalpicola daejeonensis RU-04T (98.4 %), Gryllotalpicola soli KIS12-7T (98.2 %), Gryllotalpicola kribbensis PU-02T (97.5 %), Gryllotalpicola koreensis RU-16T (97.4 %) and Gryllotalpicola reticulitermitis TS-56T (97.2 %).
31675290	6	2	theme	daejeonensis	717:728	arg1	%					743:743	98.4 %	738:743	98.4 %	738:743	The 16S rRNA gene sequence of strain 2DFW10M-5T showed the highest similarity to Gryllotalpicola daejeonensis RU-04T (98.4 %), Gryllotalpicola soli KIS12-7T (98.2 %), Gryllotalpicola kribbensis PU-02T (97.5 %), Gryllotalpicola koreensis RU-16T (97.4 %) and Gryllotalpicola reticulitermitis TS-56T (97.2 %).
31675290	4	3	theme	%	521:521	arg1	NaCl					523:526	1 % NaCl	519:526	1 % NaCl (w/v; optimum, 0 %) on Reasoner's 2A medium	519:570	The strain grew at the range of 10-37 °C (optimum, 28-30 °C) and pH 4.0-8.0 (optimum, pH 7.0) and tolerated up to 1 % NaCl (w/v; optimum, 0 %) on Reasoner's 2A medium.
31675290	7	4	theme	rRNA	966:969	arg1	sequence					976:983	the 16S rRNA gene sequence	958:983	the 16S rRNA gene sequence	958:983	The phylogenetic tree based on the 16S rRNA gene sequence revealed that strain 2DFW10M-5T fell into the radius of the genus Gryllotalpicola.
31675290	10	5	theme	isoprenoid	1279:1288	arg1	quinones					1290:1297	The detected isoprenoid quinones	1266:1297	The detected isoprenoid quinones	1266:1297	The detected isoprenoid quinones were MK-11 (61.0 %), MK-10 (33.7 %) and MK-12 (5.3 %).
31675290	10	5	theme	isoprenoid	1279:1288	arg1	MK-11					1304:1308	MK-11	1304:1308	MK-11	1304:1308	The detected isoprenoid quinones were MK-11 (61.0 %), MK-10 (33.7 %) and MK-12 (5.3 %).
31675290	2	6	theme	brevitarsis	274:284	arg1	seulensis					286:294	Protaetia brevitarsis seulensis	264:294	Protaetia brevitarsis seulensis collected in the Republic of Korea	264:329	A bacterial strain, designated 2DFW10M-5T, was isolated from gut of the larva of Protaetia brevitarsis seulensis collected in the Republic of Korea.
31675290	6	7	theme	highest	679:685	arg1	similarity					687:696	the highest similarity	675:696	the highest similarity to Gryllotalpicola daejeonensis RU-04T (98.4 %), Gryllotalpicola soli KIS12-7T (98.2 %), Gryllotalpicola kribbensis PU-02T (97.5 %), Gryllotalpicola koreensis RU-16T (97.4 %) and Gryllotalpicola reticulitermitis TS-56T (97.2 %)	675:924	The 16S rRNA gene sequence of strain 2DFW10M-5T showed the highest similarity to Gryllotalpicola daejeonensis RU-04T (98.4 %), Gryllotalpicola soli KIS12-7T (98.2 %), Gryllotalpicola kribbensis PU-02T (97.5 %), Gryllotalpicola koreensis RU-16T (97.4 %) and Gryllotalpicola reticulitermitis TS-56T (97.2 %).
31675290	13	8	theme	Gryllotalpicola	1758:1772	arg1	sp					1785:1786	the name Gryllotalpicola protaetiae sp	1749:1786	the name Gryllotalpicola protaetiae sp	1749:1786	On the basis of its genomic, phylogenetic and phenotypic properties and distinctiveness, strain 2DFW10M-5T represents a novel species of the genus Gryllotalpicola, for which the name Gryllotalpicola protaetiae sp.
31675290	6	9	theme	Gryllotalpicola	831:845	arg1	RU-16T					857:862	Gryllotalpicola koreensis RU-16T	831:862	Gryllotalpicola koreensis RU-16T (97.4 %)	831:871	The 16S rRNA gene sequence of strain 2DFW10M-5T showed the highest similarity to Gryllotalpicola daejeonensis RU-04T (98.4 %), Gryllotalpicola soli KIS12-7T (98.2 %), Gryllotalpicola kribbensis PU-02T (97.5 %), Gryllotalpicola koreensis RU-16T (97.4 %) and Gryllotalpicola reticulitermitis TS-56T (97.2 %).
31675290	6	9	theme	Gryllotalpicola	831:845	arg1	%					870:870	97.4 %	865:870	97.4 %	865:870	The 16S rRNA gene sequence of strain 2DFW10M-5T showed the highest similarity to Gryllotalpicola daejeonensis RU-04T (98.4 %), Gryllotalpicola soli KIS12-7T (98.2 %), Gryllotalpicola kribbensis PU-02T (97.5 %), Gryllotalpicola koreensis RU-16T (97.4 %) and Gryllotalpicola reticulitermitis TS-56T (97.2 %).
31675290	12	10	theme	mol	1569:1571	arg1	%					1572:1572	69.2 mol%	1564:1572	69.2 mol%	1564:1572	The DNA G+C content calculated from the genome sequence of strain 2DFW10M-5T was 69.2 mol%.
31675290	12	10	theme	mol	1569:1571	arg1	content					1495:1501	The DNA G+C content	1483:1501	The DNA G+C content calculated from the genome sequence of strain 2DFW10M-5T	1483:1558	The DNA G+C content calculated from the genome sequence of strain 2DFW10M-5T was 69.2 mol%.
31675290	7	11	theme	phylogenetic	931:942	arg1	tree					944:947	The phylogenetic tree	927:947	The phylogenetic tree based on the 16S rRNA gene sequence	927:983	The phylogenetic tree based on the 16S rRNA gene sequence revealed that strain 2DFW10M-5T fell into the radius of the genus Gryllotalpicola.
31675290	8	12	theme	fatty	1084:1088	arg1	ω-cyclohexyl-C17:0					1099:1116	ω-cyclohexyl-C17:0	1099:1116	ω-cyclohexyl-C17:0	1099:1116	The predominant fatty acid was ω-cyclohexyl-C17:0.
31675290	8	12	theme	fatty	1084:1088	arg1	acid					1090:1093	The predominant fatty acid	1068:1093	The predominant fatty acid	1068:1093	The predominant fatty acid was ω-cyclohexyl-C17:0.
31675290	4	13	theme	°C	443:444	arg1	range					428:432	the range	424:432	the range of 10-37 °C (optimum, 28-30 °C) and pH 4.0-8.0 (optimum, pH 7.0)	424:497	The strain grew at the range of 10-37 °C (optimum, 28-30 °C) and pH 4.0-8.0 (optimum, pH 7.0) and tolerated up to 1 % NaCl (w/v; optimum, 0 %) on Reasoner's 2A medium.
31675290	4	14	dep	w/v	529:531	arg1	optimum					534:540	optimum	534:540	optimum	534:540	The strain grew at the range of 10-37 °C (optimum, 28-30 °C) and pH 4.0-8.0 (optimum, pH 7.0) and tolerated up to 1 % NaCl (w/v; optimum, 0 %) on Reasoner's 2A medium.
31675290	15	15	theme	=KACC	1838:1842	arg1	113049T					1856:1862	=KACC 19316T=NBRC 113049T	1838:1862	=KACC 19316T=NBRC 113049T	1838:1862	The type strain is 2DFW10M-5T (=KACC 19316T=NBRC 113049T).
31675290	15	15	theme	=KACC	1838:1842	arg1	2DFW10M-5T					1826:1835	2DFW10M-5T	1826:1835	2DFW10M-5T (=KACC 19316T=NBRC 113049T)	1826:1863	The type strain is 2DFW10M-5T (=KACC 19316T=NBRC 113049T).
31675290	1	16	theme	Gryllotalpicola	129:143	arg1	species					153:159	the species	149:159	the species	149:159	nov., isolated from insect Protaetia brevitarsis seulensis, and emended descriptions of the genus Gryllotalpicola and the species Gryllotalpicola soli.
31675290	1	16	theme	Gryllotalpicola	129:143	arg1	descriptions					103:114	emended descriptions	95:114	emended descriptions of the genus Gryllotalpicola	95:143	nov., isolated from insect Protaetia brevitarsis seulensis, and emended descriptions of the genus Gryllotalpicola and the species Gryllotalpicola soli.
31675290	12	17	theme	2DFW10M-5T	1549:1558	arg1	sequence					1530:1537	the genome sequence	1519:1537	the genome sequence of strain 2DFW10M-5T	1519:1558	The DNA G+C content calculated from the genome sequence of strain 2DFW10M-5T was 69.2 mol%.
31675290	6	18	theme	Gryllotalpicola	877:891	arg1	TS-56T					910:915	Gryllotalpicola reticulitermitis TS-56T	877:915	Gryllotalpicola reticulitermitis TS-56T (97.2 %)	877:924	The 16S rRNA gene sequence of strain 2DFW10M-5T showed the highest similarity to Gryllotalpicola daejeonensis RU-04T (98.4 %), Gryllotalpicola soli KIS12-7T (98.2 %), Gryllotalpicola kribbensis PU-02T (97.5 %), Gryllotalpicola koreensis RU-16T (97.4 %) and Gryllotalpicola reticulitermitis TS-56T (97.2 %).
31675290	6	18	theme	Gryllotalpicola	877:891	arg1	%					923:923	97.2 %	918:923	97.2 %	918:923	The 16S rRNA gene sequence of strain 2DFW10M-5T showed the highest similarity to Gryllotalpicola daejeonensis RU-04T (98.4 %), Gryllotalpicola soli KIS12-7T (98.2 %), Gryllotalpicola kribbensis PU-02T (97.5 %), Gryllotalpicola koreensis RU-16T (97.4 %) and Gryllotalpicola reticulitermitis TS-56T (97.2 %).
31675290	6	19	dep	Gryllotalpicola	831:845	arg1	koreensis					847:855	koreensis	847:855	koreensis	847:855	The 16S rRNA gene sequence of strain 2DFW10M-5T showed the highest similarity to Gryllotalpicola daejeonensis RU-04T (98.4 %), Gryllotalpicola soli KIS12-7T (98.2 %), Gryllotalpicola kribbensis PU-02T (97.5 %), Gryllotalpicola koreensis RU-16T (97.4 %) and Gryllotalpicola reticulitermitis TS-56T (97.2 %).
31675290	11	20	with	l-serine	1426:1433	arg1	l-lysine					1453:1460	l-lysine	1453:1460	l-lysine	1453:1460	The peptidoglycan contained d- and l-alanine, d-glutamic acid, glycine, l-serine and d-lysine with l-lysine as the diamino acid.
31675290	9	21	theme	strain	1139:1144	arg1	2DFW10M-5T					1146:1155	strain 2DFW10M-5T	1139:1155	strain 2DFW10M-5T	1139:1155	The polar lipids of strain 2DFW10M-5T were diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and two unidentified lipids.
31675290	4	22	dep	optimum	482:488	arg1	pH					491:492	pH 7.0	491:496	pH 7.0	491:496	The strain grew at the range of 10-37 °C (optimum, 28-30 °C) and pH 4.0-8.0 (optimum, pH 7.0) and tolerated up to 1 % NaCl (w/v; optimum, 0 %) on Reasoner's 2A medium.
31675290	1	23	theme	Gryllotalpicola	161:175	arg1	soli					177:180	Gryllotalpicola soli	161:180	Gryllotalpicola soli	161:180	nov., isolated from insect Protaetia brevitarsis seulensis, and emended descriptions of the genus Gryllotalpicola and the species Gryllotalpicola soli.
31675290	12	24	theme	genome	1523:1528	arg1	sequence					1530:1537	the genome sequence	1519:1537	the genome sequence of strain 2DFW10M-5T	1519:1558	The DNA G+C content calculated from the genome sequence of strain 2DFW10M-5T was 69.2 mol%.
31675290	11	25	contain	contained	1372:1380	arg2	l-alanine					1389:1397	l-alanine	1389:1397	l-alanine	1389:1397	The peptidoglycan contained d- and l-alanine, d-glutamic acid, glycine, l-serine and d-lysine with l-lysine as the diamino acid.
31675290	11	25	contain	contained	1372:1380	arg2	d-					1382:1383	d-	1382:1383	d-	1382:1383	The peptidoglycan contained d- and l-alanine, d-glutamic acid, glycine, l-serine and d-lysine with l-lysine as the diamino acid.
31675290	11	25	contain	contained	1372:1380	arg1	peptidoglycan					1358:1370	The peptidoglycan	1354:1370	The peptidoglycan	1354:1370	The peptidoglycan contained d- and l-alanine, d-glutamic acid, glycine, l-serine and d-lysine with l-lysine as the diamino acid.
31675290	3	26	theme	strain	345:350	arg1	Cells					332:336	Cells	332:336	Cells of the strain	332:350	Cells of the strain were Gram-stain-positive, non-motile and rod-shaped.
31675290	4	27	theme	pH	470:471	arg1	range					428:432	the range	424:432	the range of 10-37 °C (optimum, 28-30 °C) and pH 4.0-8.0 (optimum, pH 7.0)	424:497	The strain grew at the range of 10-37 °C (optimum, 28-30 °C) and pH 4.0-8.0 (optimum, pH 7.0) and tolerated up to 1 % NaCl (w/v; optimum, 0 %) on Reasoner's 2A medium.
31675290	13	28	theme	genus	1716:1720	arg1	Gryllotalpicola					1722:1736	the genus Gryllotalpicola	1712:1736	the genus Gryllotalpicola	1712:1736	On the basis of its genomic, phylogenetic and phenotypic properties and distinctiveness, strain 2DFW10M-5T represents a novel species of the genus Gryllotalpicola, for which the name Gryllotalpicola protaetiae sp.
31675290	7	29	theme	Gryllotalpicola	1051:1065	arg1	radius					1031:1036	the radius	1027:1036	the radius of the genus Gryllotalpicola	1027:1065	The phylogenetic tree based on the 16S rRNA gene sequence revealed that strain 2DFW10M-5T fell into the radius of the genus Gryllotalpicola.
31675290	1	30	theme	Protaetia	58:66	arg1	seulensis					80:88	insect Protaetia brevitarsis seulensis	51:88	insect Protaetia brevitarsis seulensis	51:88	nov., isolated from insect Protaetia brevitarsis seulensis, and emended descriptions of the genus Gryllotalpicola and the species Gryllotalpicola soli.
31675290	12	31	theme	DNA	1487:1489	arg1	%					1572:1572	69.2 mol%	1564:1572	69.2 mol%	1564:1572	The DNA G+C content calculated from the genome sequence of strain 2DFW10M-5T was 69.2 mol%.
31675290	12	31	theme	DNA	1487:1489	arg1	content					1495:1501	The DNA G+C content	1483:1501	The DNA G+C content calculated from the genome sequence of strain 2DFW10M-5T	1483:1558	The DNA G+C content calculated from the genome sequence of strain 2DFW10M-5T was 69.2 mol%.
31675290	6	32	dep	Gryllotalpicola	787:801	arg1	kribbensis					803:812	kribbensis	803:812	kribbensis	803:812	The 16S rRNA gene sequence of strain 2DFW10M-5T showed the highest similarity to Gryllotalpicola daejeonensis RU-04T (98.4 %), Gryllotalpicola soli KIS12-7T (98.2 %), Gryllotalpicola kribbensis PU-02T (97.5 %), Gryllotalpicola koreensis RU-16T (97.4 %) and Gryllotalpicola reticulitermitis TS-56T (97.2 %).
31675290	13	33	theme	novel	1695:1699	arg1	species					1701:1707	a novel species	1693:1707	a novel species	1693:1707	On the basis of its genomic, phylogenetic and phenotypic properties and distinctiveness, strain 2DFW10M-5T represents a novel species of the genus Gryllotalpicola, for which the name Gryllotalpicola protaetiae sp.
31675290	0	34	theme	Gryllotalpicola	0:14	arg1	sp					27:28	Gryllotalpicola protaetiae sp	0:28	Gryllotalpicola protaetiae sp.	0:29	Gryllotalpicola protaetiae sp.
31675290	9	35	theme	unidentified	1211:1222	arg1	phospholipid					1224:1235	an unidentified phospholipid	1208:1235	an unidentified phospholipid	1208:1235	The polar lipids of strain 2DFW10M-5T were diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and two unidentified lipids.
31675290	13	36	dep	properties	1632:1641	arg1	the					1578:1580	the	1578:1580	the	1578:1580	On the basis of its genomic, phylogenetic and phenotypic properties and distinctiveness, strain 2DFW10M-5T represents a novel species of the genus Gryllotalpicola, for which the name Gryllotalpicola protaetiae sp.
31675290	13	36	dep	properties	1632:1641	arg1	basis					1582:1586	basis	1582:1586	basis	1582:1586	On the basis of its genomic, phylogenetic and phenotypic properties and distinctiveness, strain 2DFW10M-5T represents a novel species of the genus Gryllotalpicola, for which the name Gryllotalpicola protaetiae sp.
31675290	6	37	theme	soli	763:766	arg1	KIS12-7T					768:775	Gryllotalpicola soli KIS12-7T	747:775	Gryllotalpicola soli KIS12-7T (98.2 %)	747:784	The 16S rRNA gene sequence of strain 2DFW10M-5T showed the highest similarity to Gryllotalpicola daejeonensis RU-04T (98.4 %), Gryllotalpicola soli KIS12-7T (98.2 %), Gryllotalpicola kribbensis PU-02T (97.5 %), Gryllotalpicola koreensis RU-16T (97.4 %) and Gryllotalpicola reticulitermitis TS-56T (97.2 %).
31675290	6	37	theme	soli	763:766	arg1	%					783:783	98.2 %	778:783	98.2 %	778:783	The 16S rRNA gene sequence of strain 2DFW10M-5T showed the highest similarity to Gryllotalpicola daejeonensis RU-04T (98.4 %), Gryllotalpicola soli KIS12-7T (98.2 %), Gryllotalpicola kribbensis PU-02T (97.5 %), Gryllotalpicola koreensis RU-16T (97.4 %) and Gryllotalpicola reticulitermitis TS-56T (97.2 %).
31675290	15	38	theme	19316T=NBRC	1844:1854	arg1	113049T					1856:1862	=KACC 19316T=NBRC 113049T	1838:1862	=KACC 19316T=NBRC 113049T	1838:1862	The type strain is 2DFW10M-5T (=KACC 19316T=NBRC 113049T).
31675290	15	38	theme	19316T=NBRC	1844:1854	arg1	2DFW10M-5T					1826:1835	2DFW10M-5T	1826:1835	2DFW10M-5T (=KACC 19316T=NBRC 113049T)	1826:1863	The type strain is 2DFW10M-5T (=KACC 19316T=NBRC 113049T).
31675290	13	39	theme	strain	1664:1669	arg1	2DFW10M-5T					1671:1680	strain 2DFW10M-5T	1664:1680	strain 2DFW10M-5T	1664:1680	On the basis of its genomic, phylogenetic and phenotypic properties and distinctiveness, strain 2DFW10M-5T represents a novel species of the genus Gryllotalpicola, for which the name Gryllotalpicola protaetiae sp.
31675290	9	40	theme	unidentified	1245:1256	arg1	lipids					1258:1263	two unidentified lipids	1241:1263	two unidentified lipids	1241:1263	The polar lipids of strain 2DFW10M-5T were diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and two unidentified lipids.
31675290	11	41	with	d-	1382:1383	arg1	l-lysine					1453:1460	l-lysine	1453:1460	l-lysine	1453:1460	The peptidoglycan contained d- and l-alanine, d-glutamic acid, glycine, l-serine and d-lysine with l-lysine as the diamino acid.
31675290	1	42	dep	soli	177:180	arg1	species					153:159	the species	149:159	the species	149:159	nov., isolated from insect Protaetia brevitarsis seulensis, and emended descriptions of the genus Gryllotalpicola and the species Gryllotalpicola soli.
31675290	1	42	dep	soli	177:180	arg1	descriptions					103:114	emended descriptions	95:114	emended descriptions of the genus Gryllotalpicola	95:143	nov., isolated from insect Protaetia brevitarsis seulensis, and emended descriptions of the genus Gryllotalpicola and the species Gryllotalpicola soli.
31675290	11	43	with	l-alanine	1389:1397	arg1	l-lysine					1453:1460	l-lysine	1453:1460	l-lysine	1453:1460	The peptidoglycan contained d- and l-alanine, d-glutamic acid, glycine, l-serine and d-lysine with l-lysine as the diamino acid.
31675290	6	44	theme	gene	633:636	arg1	sequence					638:645	The 16S rRNA gene sequence	620:645	The 16S rRNA gene sequence of strain 2DFW10M-5T	620:666	The 16S rRNA gene sequence of strain 2DFW10M-5T showed the highest similarity to Gryllotalpicola daejeonensis RU-04T (98.4 %), Gryllotalpicola soli KIS12-7T (98.2 %), Gryllotalpicola kribbensis PU-02T (97.5 %), Gryllotalpicola koreensis RU-16T (97.4 %) and Gryllotalpicola reticulitermitis TS-56T (97.2 %).
31675290	2	45	theme	Korea	325:329	arg1	Republic					313:320	the Republic	309:320	the Republic of Korea	309:329	A bacterial strain, designated 2DFW10M-5T, was isolated from gut of the larva of Protaetia brevitarsis seulensis collected in the Republic of Korea.
31675290	13	46	theme	phylogenetic	1604:1615	arg1	properties					1632:1641	its genomic, phylogenetic and phenotypic properties	1591:1641	its genomic, phylogenetic and phenotypic properties	1591:1641	On the basis of its genomic, phylogenetic and phenotypic properties and distinctiveness, strain 2DFW10M-5T represents a novel species of the genus Gryllotalpicola, for which the name Gryllotalpicola protaetiae sp.
31675290	6	47	theme	16S	624:626	arg1	rRNA					628:631	The 16S rRNA	620:631	The 16S rRNA gene sequence of strain 2DFW10M-5T	620:666	The 16S rRNA gene sequence of strain 2DFW10M-5T showed the highest similarity to Gryllotalpicola daejeonensis RU-04T (98.4 %), Gryllotalpicola soli KIS12-7T (98.2 %), Gryllotalpicola kribbensis PU-02T (97.5 %), Gryllotalpicola koreensis RU-16T (97.4 %) and Gryllotalpicola reticulitermitis TS-56T (97.2 %).
31675290	13	48	theme	phenotypic	1621:1630	arg1	properties					1632:1641	its genomic, phylogenetic and phenotypic properties	1591:1641	its genomic, phylogenetic and phenotypic properties	1591:1641	On the basis of its genomic, phylogenetic and phenotypic properties and distinctiveness, strain 2DFW10M-5T represents a novel species of the genus Gryllotalpicola, for which the name Gryllotalpicola protaetiae sp.
31675290	7	49	theme	gene	971:974	arg1	sequence					976:983	the 16S rRNA gene sequence	958:983	the 16S rRNA gene sequence	958:983	The phylogenetic tree based on the 16S rRNA gene sequence revealed that strain 2DFW10M-5T fell into the radius of the genus Gryllotalpicola.
31675290	11	50	theme	diamino	1469:1475	arg1	acid					1477:1480	the diamino acid	1465:1480	the diamino acid	1465:1480	The peptidoglycan contained d- and l-alanine, d-glutamic acid, glycine, l-serine and d-lysine with l-lysine as the diamino acid.
31675290	7	51	theme	16S	962:964	arg1	rRNA					966:969	the 16S rRNA	958:969	the 16S rRNA gene sequence	958:983	The phylogenetic tree based on the 16S rRNA gene sequence revealed that strain 2DFW10M-5T fell into the radius of the genus Gryllotalpicola.
31675290	6	52	theme	Gryllotalpicola	701:715	arg1	RU-04T					730:735	Gryllotalpicola daejeonensis RU-04T	701:735	Gryllotalpicola daejeonensis RU-04T (98.4 %)	701:744	The 16S rRNA gene sequence of strain 2DFW10M-5T showed the highest similarity to Gryllotalpicola daejeonensis RU-04T (98.4 %), Gryllotalpicola soli KIS12-7T (98.2 %), Gryllotalpicola kribbensis PU-02T (97.5 %), Gryllotalpicola koreensis RU-16T (97.4 %) and Gryllotalpicola reticulitermitis TS-56T (97.2 %).
31675290	6	52	theme	Gryllotalpicola	701:715	arg1	%					743:743	98.4 %	738:743	98.4 %	738:743	The 16S rRNA gene sequence of strain 2DFW10M-5T showed the highest similarity to Gryllotalpicola daejeonensis RU-04T (98.4 %), Gryllotalpicola soli KIS12-7T (98.2 %), Gryllotalpicola kribbensis PU-02T (97.5 %), Gryllotalpicola koreensis RU-16T (97.4 %) and Gryllotalpicola reticulitermitis TS-56T (97.2 %).
31675290	2	53	theme	seulensis	286:294	arg1	larva					255:259	the larva	251:259	the larva of Protaetia brevitarsis seulensis collected in the Republic of Korea	251:329	A bacterial strain, designated 2DFW10M-5T, was isolated from gut of the larva of Protaetia brevitarsis seulensis collected in the Republic of Korea.
31675290	4	54	theme	1 	519:520	arg1	%					521:521	%	521:521	%	521:521	The strain grew at the range of 10-37 °C (optimum, 28-30 °C) and pH 4.0-8.0 (optimum, pH 7.0) and tolerated up to 1 % NaCl (w/v; optimum, 0 %) on Reasoner's 2A medium.
31675290	13	55	theme	genomic	1595:1601	arg1	properties					1632:1641	its genomic, phylogenetic and phenotypic properties	1591:1641	its genomic, phylogenetic and phenotypic properties	1591:1641	On the basis of its genomic, phylogenetic and phenotypic properties and distinctiveness, strain 2DFW10M-5T represents a novel species of the genus Gryllotalpicola, for which the name Gryllotalpicola protaetiae sp.
31675290	10	56	theme	detected	1270:1277	arg1	quinones					1290:1297	The detected isoprenoid quinones	1266:1297	The detected isoprenoid quinones	1266:1297	The detected isoprenoid quinones were MK-11 (61.0 %), MK-10 (33.7 %) and MK-12 (5.3 %).
31675290	10	56	theme	detected	1270:1277	arg1	MK-11					1304:1308	MK-11	1304:1308	MK-11	1304:1308	The detected isoprenoid quinones were MK-11 (61.0 %), MK-10 (33.7 %) and MK-12 (5.3 %).
31675290	2	57	theme	Protaetia	264:272	arg1	seulensis					286:294	Protaetia brevitarsis seulensis	264:294	Protaetia brevitarsis seulensis collected in the Republic of Korea	264:329	A bacterial strain, designated 2DFW10M-5T, was isolated from gut of the larva of Protaetia brevitarsis seulensis collected in the Republic of Korea.
31675290	13	58	theme	name	1753:1756	arg1	sp					1785:1786	the name Gryllotalpicola protaetiae sp	1749:1786	the name Gryllotalpicola protaetiae sp	1749:1786	On the basis of its genomic, phylogenetic and phenotypic properties and distinctiveness, strain 2DFW10M-5T represents a novel species of the genus Gryllotalpicola, for which the name Gryllotalpicola protaetiae sp.
31675290	6	59	theme	2DFW10M-5T	657:666	arg1	sequence					638:645	The 16S rRNA gene sequence	620:645	The 16S rRNA gene sequence of strain 2DFW10M-5T	620:666	The 16S rRNA gene sequence of strain 2DFW10M-5T showed the highest similarity to Gryllotalpicola daejeonensis RU-04T (98.4 %), Gryllotalpicola soli KIS12-7T (98.2 %), Gryllotalpicola kribbensis PU-02T (97.5 %), Gryllotalpicola koreensis RU-16T (97.4 %) and Gryllotalpicola reticulitermitis TS-56T (97.2 %).
31675290	8	60	theme	predominant	1072:1082	arg1	ω-cyclohexyl-C17:0					1099:1116	ω-cyclohexyl-C17:0	1099:1116	ω-cyclohexyl-C17:0	1099:1116	The predominant fatty acid was ω-cyclohexyl-C17:0.
31675290	8	60	theme	predominant	1072:1082	arg1	acid					1090:1093	The predominant fatty acid	1068:1093	The predominant fatty acid	1068:1093	The predominant fatty acid was ω-cyclohexyl-C17:0.
31675290	13	61	theme	Gryllotalpicola	1722:1736	arg1	species					1701:1707	a novel species	1693:1707	a novel species	1693:1707	On the basis of its genomic, phylogenetic and phenotypic properties and distinctiveness, strain 2DFW10M-5T represents a novel species of the genus Gryllotalpicola, for which the name Gryllotalpicola protaetiae sp.
31675290	13	62	theme	protaetiae	1774:1783	arg1	sp					1785:1786	the name Gryllotalpicola protaetiae sp	1749:1786	the name Gryllotalpicola protaetiae sp	1749:1786	On the basis of its genomic, phylogenetic and phenotypic properties and distinctiveness, strain 2DFW10M-5T represents a novel species of the genus Gryllotalpicola, for which the name Gryllotalpicola protaetiae sp.
31675290	9	63	theme	polar	1123:1127	arg1	diphosphatidylglycerol					1162:1183	diphosphatidylglycerol	1162:1183	diphosphatidylglycerol	1162:1183	The polar lipids of strain 2DFW10M-5T were diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and two unidentified lipids.
31675290	9	63	theme	polar	1123:1127	arg1	lipids					1129:1134	The polar lipids	1119:1134	The polar lipids of strain 2DFW10M-5T	1119:1155	The polar lipids of strain 2DFW10M-5T were diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and two unidentified lipids.
31675290	2	64	theme	larva	255:259	arg1	gut					244:246	gut	244:246	gut of the larva of Protaetia brevitarsis seulensis collected in the Republic of Korea	244:329	A bacterial strain, designated 2DFW10M-5T, was isolated from gut of the larva of Protaetia brevitarsis seulensis collected in the Republic of Korea.
31675290	1	65	theme	genus	123:127	arg1	Gryllotalpicola					129:143	the genus Gryllotalpicola	119:143	the genus Gryllotalpicola	119:143	nov., isolated from insect Protaetia brevitarsis seulensis, and emended descriptions of the genus Gryllotalpicola and the species Gryllotalpicola soli.
31675290	6	66	theme	reticulitermitis	893:908	arg1	TS-56T					910:915	Gryllotalpicola reticulitermitis TS-56T	877:915	Gryllotalpicola reticulitermitis TS-56T (97.2 %)	877:924	The 16S rRNA gene sequence of strain 2DFW10M-5T showed the highest similarity to Gryllotalpicola daejeonensis RU-04T (98.4 %), Gryllotalpicola soli KIS12-7T (98.2 %), Gryllotalpicola kribbensis PU-02T (97.5 %), Gryllotalpicola koreensis RU-16T (97.4 %) and Gryllotalpicola reticulitermitis TS-56T (97.2 %).
31675290	6	66	theme	reticulitermitis	893:908	arg1	%					923:923	97.2 %	918:923	97.2 %	918:923	The 16S rRNA gene sequence of strain 2DFW10M-5T showed the highest similarity to Gryllotalpicola daejeonensis RU-04T (98.4 %), Gryllotalpicola soli KIS12-7T (98.2 %), Gryllotalpicola kribbensis PU-02T (97.5 %), Gryllotalpicola koreensis RU-16T (97.4 %) and Gryllotalpicola reticulitermitis TS-56T (97.2 %).
31675290	4	67	dep	NaCl	523:526	arg1	up					513:514	up	513:514	up	513:514	The strain grew at the range of 10-37 °C (optimum, 28-30 °C) and pH 4.0-8.0 (optimum, pH 7.0) and tolerated up to 1 % NaCl (w/v; optimum, 0 %) on Reasoner's 2A medium.
31675290	4	67	dep	NaCl	523:526	arg1	%					545:545	0 %	543:545	0 %	543:545	The strain grew at the range of 10-37 °C (optimum, 28-30 °C) and pH 4.0-8.0 (optimum, pH 7.0) and tolerated up to 1 % NaCl (w/v; optimum, 0 %) on Reasoner's 2A medium.
31675290	4	67	dep	NaCl	523:526	arg1	w/v					529:531	w/v	529:531	w/v	529:531	The strain grew at the range of 10-37 °C (optimum, 28-30 °C) and pH 4.0-8.0 (optimum, pH 7.0) and tolerated up to 1 % NaCl (w/v; optimum, 0 %) on Reasoner's 2A medium.
31675290	12	68	theme	strain	1542:1547	arg1	2DFW10M-5T					1549:1558	strain 2DFW10M-5T	1542:1558	strain 2DFW10M-5T	1542:1558	The DNA G+C content calculated from the genome sequence of strain 2DFW10M-5T was 69.2 mol%.
31675290	4	69	theme	2A	562:563	arg1	medium					565:570	Reasoner's 2A medium	551:570	Reasoner's 2A medium	551:570	The strain grew at the range of 10-37 °C (optimum, 28-30 °C) and pH 4.0-8.0 (optimum, pH 7.0) and tolerated up to 1 % NaCl (w/v; optimum, 0 %) on Reasoner's 2A medium.
31675290	9	70	theme	2DFW10M-5T	1146:1155	arg1	diphosphatidylglycerol					1162:1183	diphosphatidylglycerol	1162:1183	diphosphatidylglycerol	1162:1183	The polar lipids of strain 2DFW10M-5T were diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and two unidentified lipids.
31675290	9	70	theme	2DFW10M-5T	1146:1155	arg1	lipids					1129:1134	The polar lipids	1119:1134	The polar lipids of strain 2DFW10M-5T	1119:1155	The polar lipids of strain 2DFW10M-5T were diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and two unidentified lipids.
31675290	2	71	attach	isolated	230:237	arg2	strain					195:200	A bacterial strain	183:200	A bacterial strain	183:200	A bacterial strain, designated 2DFW10M-5T, was isolated from gut of the larva of Protaetia brevitarsis seulensis collected in the Republic of Korea.
31675290	2	71	attach	isolated	230:237	arg1	gut					244:246	gut	244:246	gut of the larva of Protaetia brevitarsis seulensis collected in the Republic of Korea	244:329	A bacterial strain, designated 2DFW10M-5T, was isolated from gut of the larva of Protaetia brevitarsis seulensis collected in the Republic of Korea.
31675290	7	72	theme	genus	1045:1049	arg1	Gryllotalpicola					1051:1065	the genus Gryllotalpicola	1041:1065	the genus Gryllotalpicola	1041:1065	The phylogenetic tree based on the 16S rRNA gene sequence revealed that strain 2DFW10M-5T fell into the radius of the genus Gryllotalpicola.
31675290	1	73	theme	insect	51:56	arg1	seulensis					80:88	insect Protaetia brevitarsis seulensis	51:88	insect Protaetia brevitarsis seulensis	51:88	nov., isolated from insect Protaetia brevitarsis seulensis, and emended descriptions of the genus Gryllotalpicola and the species Gryllotalpicola soli.
31675290	1	74	attach	isolated	37:44	arg1	seulensis					80:88	insect Protaetia brevitarsis seulensis	51:88	insect Protaetia brevitarsis seulensis	51:88	nov., isolated from insect Protaetia brevitarsis seulensis, and emended descriptions of the genus Gryllotalpicola and the species Gryllotalpicola soli.
31675290	1	74	attach	isolated	37:44	arg2	nov.					31:34	nov.	31:34	nov.	31:34	nov., isolated from insect Protaetia brevitarsis seulensis, and emended descriptions of the genus Gryllotalpicola and the species Gryllotalpicola soli.
31675290	1	75	theme	brevitarsis	68:78	arg1	seulensis					80:88	insect Protaetia brevitarsis seulensis	51:88	insect Protaetia brevitarsis seulensis	51:88	nov., isolated from insect Protaetia brevitarsis seulensis, and emended descriptions of the genus Gryllotalpicola and the species Gryllotalpicola soli.
31675290	0	76	theme	protaetiae	16:25	arg1	sp					27:28	Gryllotalpicola protaetiae sp	0:28	Gryllotalpicola protaetiae sp.	0:29	Gryllotalpicola protaetiae sp.
31675290	12	77	theme	G+C	1491:1493	arg1	%					1572:1572	69.2 mol%	1564:1572	69.2 mol%	1564:1572	The DNA G+C content calculated from the genome sequence of strain 2DFW10M-5T was 69.2 mol%.
31675290	12	77	theme	G+C	1491:1493	arg1	content					1495:1501	The DNA G+C content	1483:1501	The DNA G+C content calculated from the genome sequence of strain 2DFW10M-5T	1483:1558	The DNA G+C content calculated from the genome sequence of strain 2DFW10M-5T was 69.2 mol%.
31675290	1	78	theme	emended	95:101	arg1	descriptions					103:114	emended descriptions	95:114	emended descriptions of the genus Gryllotalpicola	95:143	nov., isolated from insect Protaetia brevitarsis seulensis, and emended descriptions of the genus Gryllotalpicola and the species Gryllotalpicola soli.
31675290	11	79	with	acid	1411:1414	arg1	l-lysine					1453:1460	l-lysine	1453:1460	l-lysine	1453:1460	The peptidoglycan contained d- and l-alanine, d-glutamic acid, glycine, l-serine and d-lysine with l-lysine as the diamino acid.
31675290	2	80	theme	bacterial	185:193	arg1	strain					195:200	A bacterial strain	183:200	A bacterial strain	183:200	A bacterial strain, designated 2DFW10M-5T, was isolated from gut of the larva of Protaetia brevitarsis seulensis collected in the Republic of Korea.
31675290	7	81	theme	strain	999:1004	arg1	2DFW10M-5T					1006:1015	strain 2DFW10M-5T	999:1015	strain 2DFW10M-5T	999:1015	The phylogenetic tree based on the 16S rRNA gene sequence revealed that strain 2DFW10M-5T fell into the radius of the genus Gryllotalpicola.
31675290	4	82	dep	optimum	447:453	arg1	°C					462:463	28-30 °C	456:463	28-30 °C	456:463	The strain grew at the range of 10-37 °C (optimum, 28-30 °C) and pH 4.0-8.0 (optimum, pH 7.0) and tolerated up to 1 % NaCl (w/v; optimum, 0 %) on Reasoner's 2A medium.
31675290	6	83	theme	Gryllotalpicola	747:761	arg1	KIS12-7T					768:775	Gryllotalpicola soli KIS12-7T	747:775	Gryllotalpicola soli KIS12-7T (98.2 %)	747:784	The 16S rRNA gene sequence of strain 2DFW10M-5T showed the highest similarity to Gryllotalpicola daejeonensis RU-04T (98.4 %), Gryllotalpicola soli KIS12-7T (98.2 %), Gryllotalpicola kribbensis PU-02T (97.5 %), Gryllotalpicola koreensis RU-16T (97.4 %) and Gryllotalpicola reticulitermitis TS-56T (97.2 %).
31675290	6	83	theme	Gryllotalpicola	747:761	arg1	%					783:783	98.2 %	778:783	98.2 %	778:783	The 16S rRNA gene sequence of strain 2DFW10M-5T showed the highest similarity to Gryllotalpicola daejeonensis RU-04T (98.4 %), Gryllotalpicola soli KIS12-7T (98.2 %), Gryllotalpicola kribbensis PU-02T (97.5 %), Gryllotalpicola koreensis RU-16T (97.4 %) and Gryllotalpicola reticulitermitis TS-56T (97.2 %).
31675290	6	84	theme	strain	650:655	arg1	2DFW10M-5T					657:666	strain 2DFW10M-5T	650:666	strain 2DFW10M-5T	650:666	The 16S rRNA gene sequence of strain 2DFW10M-5T showed the highest similarity to Gryllotalpicola daejeonensis RU-04T (98.4 %), Gryllotalpicola soli KIS12-7T (98.2 %), Gryllotalpicola kribbensis PU-02T (97.5 %), Gryllotalpicola koreensis RU-16T (97.4 %) and Gryllotalpicola reticulitermitis TS-56T (97.2 %).
31675290	15	85	theme	type	1811:1814	arg1	2DFW10M-5T					1826:1835	2DFW10M-5T	1826:1835	2DFW10M-5T (=KACC 19316T=NBRC 113049T)	1826:1863	The type strain is 2DFW10M-5T (=KACC 19316T=NBRC 113049T).
31675290	15	85	theme	type	1811:1814	arg1	strain					1816:1821	The type strain	1807:1821	The type strain	1807:1821	The type strain is 2DFW10M-5T (=KACC 19316T=NBRC 113049T).
31675290	11	86	theme	d-glutamic	1400:1409	arg1	acid					1411:1414	d-glutamic acid	1400:1414	d-glutamic acid	1400:1414	The peptidoglycan contained d- and l-alanine, d-glutamic acid, glycine, l-serine and d-lysine with l-lysine as the diamino acid.
31675290	11	87	with	d-lysine	1439:1446	arg1	l-lysine					1453:1460	l-lysine	1453:1460	l-lysine	1453:1460	The peptidoglycan contained d- and l-alanine, d-glutamic acid, glycine, l-serine and d-lysine with l-lysine as the diamino acid.
31675290	6	88	theme	rRNA	628:631	arg1	sequence					638:645	The 16S rRNA gene sequence	620:645	The 16S rRNA gene sequence of strain 2DFW10M-5T	620:666	The 16S rRNA gene sequence of strain 2DFW10M-5T showed the highest similarity to Gryllotalpicola daejeonensis RU-04T (98.4 %), Gryllotalpicola soli KIS12-7T (98.2 %), Gryllotalpicola kribbensis PU-02T (97.5 %), Gryllotalpicola koreensis RU-16T (97.4 %) and Gryllotalpicola reticulitermitis TS-56T (97.2 %).
33999796	4	0	theme	Phylogenetic	414:425	arg1	reconstructions					427:441	Phylogenetic reconstructions	414:441	Phylogenetic reconstructions	414:441	Phylogenetic reconstructions based on 16S rRNA gene sequences and genome-based on amino acid sequences of 118 single copy genes referred strain 15G-AUS-rotT to the family Microbacteriaceae and therein to the so-called subcluster Luna-1.
33999796	4	1	theme	rRNA	456:459	arg1	sequences					466:474	16S rRNA gene sequences	452:474	16S rRNA gene sequences	452:474	Phylogenetic reconstructions based on 16S rRNA gene sequences and genome-based on amino acid sequences of 118 single copy genes referred strain 15G-AUS-rotT to the family Microbacteriaceae and therein to the so-called subcluster Luna-1.
33999796	11	2	theme	genome	1123:1128	arg1	sequence					1130:1137	The closed genome sequence	1112:1137	The closed genome sequence of the strain	1112:1151	The closed genome sequence of the strain was very small (1.4 Mbp) and had a DNA G+C content of 54.8 mol%.
33999796	11	2	theme	genome	1123:1128	arg1	small					1162:1166	small	1162:1166	small	1162:1166	The closed genome sequence of the strain was very small (1.4 Mbp) and had a DNA G+C content of 54.8 mol%.
33999796	11	2	theme	genome	1123:1128	arg1	Mbp					1173:1175	1.4 Mbp	1169:1175	1.4 Mbp	1169:1175	The closed genome sequence of the strain was very small (1.4 Mbp) and had a DNA G+C content of 54.8 mol%.
33999796	5	3	theme	phylogenetic	668:679	arg1	tree					681:684	The genome-based phylogenetic tree	651:684	The genome-based phylogenetic tree	651:684	The genome-based phylogenetic tree showed that the new strain represents a putative new genus.
33999796	7	4	theme	broad	866:870	arg1	substrates					883:892	substrates	883:892	substrates	883:892	They metabolized a broad variety of substrates.
33999796	7	4	theme	broad	866:870	arg1	variety					872:878	a broad variety	864:878	a broad variety of substrates	864:892	They metabolized a broad variety of substrates.
33999796	4	5	theme	genes	536:540	arg1	sequences					507:515	amino acid sequences	496:515	amino acid sequences of 118 single copy genes referred strain 15G-AUS-rotT to the family Microbacteriaceae and therein to the so-called subcluster Luna-1	496:648	Phylogenetic reconstructions based on 16S rRNA gene sequences and genome-based on amino acid sequences of 118 single copy genes referred strain 15G-AUS-rotT to the family Microbacteriaceae and therein to the so-called subcluster Luna-1.
33999796	3	6	theme	%	299:299	arg1	similarities					278:289	16S rRNA gene sequence similarities	255:289	16S rRNA gene sequence similarities of 98.7 % to Candidatus Aquiluna rubra and of 96.6 and 96.7 % to the two validly described species of the genus Rhodoluna	255:411	The strain showed 16S rRNA gene sequence similarities of 98.7 % to Candidatus Aquiluna rubra and of 96.6 and 96.7 % to the two validly described species of the genus Rhodoluna.
33999796	4	7	theme	copy	531:534	arg1	genes					536:540	118 single copy genes	520:540	118 single copy genes referred strain 15G-AUS-rotT to the family Microbacteriaceae and therein to the so-called subcluster Luna-1	520:648	Phylogenetic reconstructions based on 16S rRNA gene sequences and genome-based on amino acid sequences of 118 single copy genes referred strain 15G-AUS-rotT to the family Microbacteriaceae and therein to the so-called subcluster Luna-1.
33999796	5	8	theme	new	702:704	arg1	strain					706:711	the new strain	698:711	the new strain	698:711	The genome-based phylogenetic tree showed that the new strain represents a putative new genus.
33999796	10	9	theme	peptidoglycan	1057:1069	arg1	structure					1071:1079	The peptidoglycan structure	1053:1079	The peptidoglycan structure	1053:1079	The peptidoglycan structure belonged to an unusual B type.
33999796	8	10	theme	cells	924:928	arg1	acids					907:911	Major fatty acids	895:911	Major fatty acids (>10 %) of cells	895:928	Major fatty acids (>10 %) of cells were iso-C16 : 0, antiso-C15 : 0 and iso-C14 : 0.
33999796	8	10	theme	cells	924:928	arg1	%					918:918	>10 %	914:918	>10 %	914:918	Major fatty acids (>10 %) of cells were iso-C16 : 0, antiso-C15 : 0 and iso-C14 : 0.
33999796	9	11	theme	major	984:988	arg1	MK-11					1014:1018	MK-11	1014:1018	MK-11	1014:1018	The major respiratory quinone was MK-11 and a minor component was MK-10.
33999796	9	11	theme	major	984:988	arg1	quinone					1002:1008	The major respiratory quinone	980:1008	The major respiratory quinone	980:1008	The major respiratory quinone was MK-11 and a minor component was MK-10.
33999796	4	12	theme	strain	551:556	arg1	15G-AUS-rotT					558:569	strain 15G-AUS-rotT	551:569	strain 15G-AUS-rotT	551:569	Phylogenetic reconstructions based on 16S rRNA gene sequences and genome-based on amino acid sequences of 118 single copy genes referred strain 15G-AUS-rotT to the family Microbacteriaceae and therein to the so-called subcluster Luna-1.
33999796	13	13	theme	strain	1459:1464	arg1	characteristics					1436:1450	the characteristics	1432:1450	the characteristics of the strain	1432:1464	Based on the characteristics of the strain, a new genus and a new species termed Aquiluna borgnonia is proposed for strain 15G-AUS-rotT (=DSM 107803T=JCM 32974T).
33999796	12	14	theme	light-driven	1300:1311	arg1	proton					1313:1318	the complete light-driven proton	1287:1318	the complete light-driven proton pumping actinorhodopsin/retinal system, which were located at three different positions of the genome	1287:1420	An interesting feature was the presence of genes putatively encoding the complete light-driven proton pumping actinorhodopsin/retinal system, which were located at three different positions of the genome.
33999796	8	15	theme	Major	895:899	arg1	acids					907:911	Major fatty acids	895:911	Major fatty acids (>10 %) of cells	895:928	Major fatty acids (>10 %) of cells were iso-C16 : 0, antiso-C15 : 0 and iso-C14 : 0.
33999796	8	15	theme	Major	895:899	arg1	%					918:918	>10 %	914:918	>10 %	914:918	Major fatty acids (>10 %) of cells were iso-C16 : 0, antiso-C15 : 0 and iso-C14 : 0.
33999796	12	16	theme	genes	1261:1265	arg1	presence					1249:1256	the presence	1245:1256	the presence of genes putatively encoding the complete light-driven proton pumping actinorhodopsin/retinal system, which were located at three different positions of the genome	1245:1420	An interesting feature was the presence of genes putatively encoding the complete light-driven proton pumping actinorhodopsin/retinal system, which were located at three different positions of the genome.
33999796	12	16	theme	genes	1261:1265	arg1	feature					1233:1239	An interesting feature	1218:1239	An interesting feature	1218:1239	An interesting feature was the presence of genes putatively encoding the complete light-driven proton pumping actinorhodopsin/retinal system, which were located at three different positions of the genome.
33999796	9	17	theme	respiratory	990:1000	arg1	MK-11					1014:1018	MK-11	1014:1018	MK-11	1014:1018	The major respiratory quinone was MK-11 and a minor component was MK-10.
33999796	9	17	theme	respiratory	990:1000	arg1	quinone					1002:1008	The major respiratory quinone	980:1008	The major respiratory quinone	980:1008	The major respiratory quinone was MK-11 and a minor component was MK-10.
33999796	11	18	dep	small	1162:1166	arg1	sequence					1130:1137	The closed genome sequence	1112:1137	The closed genome sequence of the strain	1112:1151	The closed genome sequence of the strain was very small (1.4 Mbp) and had a DNA G+C content of 54.8 mol%.
33999796	11	18	dep	small	1162:1166	arg1	small					1162:1166	small	1162:1166	small	1162:1166	The closed genome sequence of the strain was very small (1.4 Mbp) and had a DNA G+C content of 54.8 mol%.
33999796	11	18	dep	small	1162:1166	arg1	Mbp					1173:1175	1.4 Mbp	1169:1175	1.4 Mbp	1169:1175	The closed genome sequence of the strain was very small (1.4 Mbp) and had a DNA G+C content of 54.8 mol%.
33999796	12	19	theme	complete	1291:1298	arg1	proton					1313:1318	the complete light-driven proton	1287:1318	the complete light-driven proton pumping actinorhodopsin/retinal system, which were located at three different positions of the genome	1287:1420	An interesting feature was the presence of genes putatively encoding the complete light-driven proton pumping actinorhodopsin/retinal system, which were located at three different positions of the genome.
33999796	11	20	theme	DNA	1188:1190	arg1	content					1196:1202	a DNA G+C content	1186:1202	a DNA G+C content of 54.8 mol%	1186:1215	The closed genome sequence of the strain was very small (1.4 Mbp) and had a DNA G+C content of 54.8 mol%.
33999796	12	21	located	located	1371:1377	arg1	positions					1398:1406	three different positions	1382:1406	three different positions of the genome	1382:1420	An interesting feature was the presence of genes putatively encoding the complete light-driven proton pumping actinorhodopsin/retinal system, which were located at three different positions of the genome.
33999796	12	21	located	located	1371:1377	arg2	system					1352:1357	actinorhodopsin/retinal system	1328:1357	actinorhodopsin/retinal system	1328:1357	An interesting feature was the presence of genes putatively encoding the complete light-driven proton pumping actinorhodopsin/retinal system, which were located at three different positions of the genome.
33999796	11	22	theme	strain	1146:1151	arg1	sequence					1130:1137	The closed genome sequence	1112:1137	The closed genome sequence of the strain	1112:1151	The closed genome sequence of the strain was very small (1.4 Mbp) and had a DNA G+C content of 54.8 mol%.
33999796	11	22	theme	strain	1146:1151	arg1	small					1162:1166	small	1162:1166	small	1162:1166	The closed genome sequence of the strain was very small (1.4 Mbp) and had a DNA G+C content of 54.8 mol%.
33999796	11	22	theme	strain	1146:1151	arg1	Mbp					1173:1175	1.4 Mbp	1169:1175	1.4 Mbp	1169:1175	The closed genome sequence of the strain was very small (1.4 Mbp) and had a DNA G+C content of 54.8 mol%.
33999796	2	23	attach	isolated	172:179	arg1	pond					200:203	pond	200:203	pond	200:203	The actinobacterial strain 15G-AUS-rotT was isolated from an artificial pond located near Salzburg, Austria.
33999796	2	23	attach	isolated	172:179	arg2	15G-AUS-rotT					155:166	The actinobacterial strain 15G-AUS-rotT	128:166	The actinobacterial strain 15G-AUS-rotT	128:166	The actinobacterial strain 15G-AUS-rotT was isolated from an artificial pond located near Salzburg, Austria.
33999796	4	24	theme	so-called	622:630	arg1	Luna-1					643:648	the so-called subcluster Luna-1	618:648	the so-called subcluster Luna-1	618:648	Phylogenetic reconstructions based on 16S rRNA gene sequences and genome-based on amino acid sequences of 118 single copy genes referred strain 15G-AUS-rotT to the family Microbacteriaceae and therein to the so-called subcluster Luna-1.
33999796	11	25	theme	54.8 mol	1207:1214	arg1	%					1215:1215	54.8 mol%	1207:1215	54.8 mol%	1207:1215	The closed genome sequence of the strain was very small (1.4 Mbp) and had a DNA G+C content of 54.8 mol%.
33999796	4	26	theme	16S	452:454	arg1	sequences					466:474	16S rRNA gene sequences	452:474	16S rRNA gene sequences	452:474	Phylogenetic reconstructions based on 16S rRNA gene sequences and genome-based on amino acid sequences of 118 single copy genes referred strain 15G-AUS-rotT to the family Microbacteriaceae and therein to the so-called subcluster Luna-1.
33999796	11	27	theme	G+C	1192:1194	arg1	content					1196:1202	a DNA G+C content	1186:1202	a DNA G+C content of 54.8 mol%	1186:1215	The closed genome sequence of the strain was very small (1.4 Mbp) and had a DNA G+C content of 54.8 mol%.
33999796	6	28	theme	15G-AUS-rotT	765:776	arg1	Cultures					746:753	Cultures	746:753	Cultures of strain 15G-AUS-rotT	746:776	Cultures of strain 15G-AUS-rotT were light red pigmented and comprised very small, rod-shaped cells.
33999796	3	29	theme	%	351:351	arg1	similarities					278:289	16S rRNA gene sequence similarities	255:289	16S rRNA gene sequence similarities of 98.7 % to Candidatus Aquiluna rubra and of 96.6 and 96.7 % to the two validly described species of the genus Rhodoluna	255:411	The strain showed 16S rRNA gene sequence similarities of 98.7 % to Candidatus Aquiluna rubra and of 96.6 and 96.7 % to the two validly described species of the genus Rhodoluna.
33999796	11	30	contain	had	1182:1184	arg2	content					1196:1202	a DNA G+C content	1186:1202	a DNA G+C content of 54.8 mol%	1186:1215	The closed genome sequence of the strain was very small (1.4 Mbp) and had a DNA G+C content of 54.8 mol%.
33999796	11	30	contain	had	1182:1184	arg1	sequence					1130:1137	The closed genome sequence	1112:1137	The closed genome sequence of the strain	1112:1151	The closed genome sequence of the strain was very small (1.4 Mbp) and had a DNA G+C content of 54.8 mol%.
33999796	11	30	contain	had	1182:1184	arg1	small					1162:1166	small	1162:1166	small	1162:1166	The closed genome sequence of the strain was very small (1.4 Mbp) and had a DNA G+C content of 54.8 mol%.
33999796	11	30	contain	had	1182:1184	arg1	Mbp					1173:1175	1.4 Mbp	1169:1175	1.4 Mbp	1169:1175	The closed genome sequence of the strain was very small (1.4 Mbp) and had a DNA G+C content of 54.8 mol%.
33999796	0	31	theme	borgnonia	9:17	arg1	nov.					24:27	Aquiluna borgnonia gen. nov.	0:27	Aquiluna borgnonia gen. nov.	0:27	Aquiluna borgnonia gen. nov., sp.
33999796	13	32	theme	=DSM	1560:1563	arg1	15G-AUS-rotT					1546:1557	strain 15G-AUS-rotT	1539:1557	strain 15G-AUS-rotT (=DSM 107803T=JCM 32974T)	1539:1583	Based on the characteristics of the strain, a new genus and a new species termed Aquiluna borgnonia is proposed for strain 15G-AUS-rotT (=DSM 107803T=JCM 32974T).
33999796	13	32	theme	=DSM	1560:1563	arg1	32974T					1577:1582	=DSM 107803T=JCM 32974T	1560:1582	=DSM 107803T=JCM 32974T	1560:1582	Based on the characteristics of the strain, a new genus and a new species termed Aquiluna borgnonia is proposed for strain 15G-AUS-rotT (=DSM 107803T=JCM 32974T).
33999796	6	33	theme	strain	758:763	arg1	15G-AUS-rotT					765:776	strain 15G-AUS-rotT	758:776	strain 15G-AUS-rotT	758:776	Cultures of strain 15G-AUS-rotT were light red pigmented and comprised very small, rod-shaped cells.
33999796	11	34	theme	closed	1116:1121	arg1	sequence					1130:1137	The closed genome sequence	1112:1137	The closed genome sequence of the strain	1112:1151	The closed genome sequence of the strain was very small (1.4 Mbp) and had a DNA G+C content of 54.8 mol%.
33999796	11	34	theme	closed	1116:1121	arg1	small					1162:1166	small	1162:1166	small	1162:1166	The closed genome sequence of the strain was very small (1.4 Mbp) and had a DNA G+C content of 54.8 mol%.
33999796	11	34	theme	closed	1116:1121	arg1	Mbp					1173:1175	1.4 Mbp	1169:1175	1.4 Mbp	1169:1175	The closed genome sequence of the strain was very small (1.4 Mbp) and had a DNA G+C content of 54.8 mol%.
33999796	3	35	theme	described	372:380	arg1	species					382:388	the two validly described species	356:388	the two validly described species of the genus Rhodoluna	356:411	The strain showed 16S rRNA gene sequence similarities of 98.7 % to Candidatus Aquiluna rubra and of 96.6 and 96.7 % to the two validly described species of the genus Rhodoluna.
33999796	0	36	theme	Aquiluna	0:7	arg1	nov.					24:27	Aquiluna borgnonia gen. nov.	0:27	Aquiluna borgnonia gen. nov.	0:27	Aquiluna borgnonia gen. nov., sp.
33999796	13	37	theme	107803T=JCM	1565:1575	arg1	15G-AUS-rotT					1546:1557	strain 15G-AUS-rotT	1539:1557	strain 15G-AUS-rotT (=DSM 107803T=JCM 32974T)	1539:1583	Based on the characteristics of the strain, a new genus and a new species termed Aquiluna borgnonia is proposed for strain 15G-AUS-rotT (=DSM 107803T=JCM 32974T).
33999796	13	37	theme	107803T=JCM	1565:1575	arg1	32974T					1577:1582	=DSM 107803T=JCM 32974T	1560:1582	=DSM 107803T=JCM 32974T	1560:1582	Based on the characteristics of the strain, a new genus and a new species termed Aquiluna borgnonia is proposed for strain 15G-AUS-rotT (=DSM 107803T=JCM 32974T).
33999796	11	38	theme	%	1215:1215	arg1	content					1196:1202	a DNA G+C content	1186:1202	a DNA G+C content of 54.8 mol%	1186:1215	The closed genome sequence of the strain was very small (1.4 Mbp) and had a DNA G+C content of 54.8 mol%.
33999796	1	39	theme	Microbacteriaceae	54:70	arg1	lineage					72:78	a Microbacteriaceae lineage	52:78	a Microbacteriaceae lineage of freshwater bacteria with small genome sizes	52:125	nov., a member of a Microbacteriaceae lineage of freshwater bacteria with small genome sizes.
33999796	4	40	theme	subcluster	632:641	arg1	Luna-1					643:648	the so-called subcluster Luna-1	618:648	the so-called subcluster Luna-1	618:648	Phylogenetic reconstructions based on 16S rRNA gene sequences and genome-based on amino acid sequences of 118 single copy genes referred strain 15G-AUS-rotT to the family Microbacteriaceae and therein to the so-called subcluster Luna-1.
33999796	12	41	from	positions	1398:1406	arg1	located					1371:1377	located	1371:1377	located	1371:1377	An interesting feature was the presence of genes putatively encoding the complete light-driven proton pumping actinorhodopsin/retinal system, which were located at three different positions of the genome.
33999796	3	42	theme	16S	255:257	arg1	rRNA					259:262	16S rRNA	255:262	16S rRNA gene sequence similarities of 98.7 % to Candidatus Aquiluna rubra and of 96.6 and 96.7 % to the two validly described species of the genus Rhodoluna	255:411	The strain showed 16S rRNA gene sequence similarities of 98.7 % to Candidatus Aquiluna rubra and of 96.6 and 96.7 % to the two validly described species of the genus Rhodoluna.
33999796	1	43	theme	lineage	72:78	arg1	member					42:47	a member	40:47	a member of a Microbacteriaceae lineage of freshwater bacteria with small genome sizes	40:125	nov., a member of a Microbacteriaceae lineage of freshwater bacteria with small genome sizes.
33999796	1	43	theme	lineage	72:78	arg1	nov.					34:37	nov.	34:37	nov.	34:37	nov., a member of a Microbacteriaceae lineage of freshwater bacteria with small genome sizes.
33999796	8	44	dep	iso-C16 	935:942	arg1	iso-C14 					967:974	iso-C14 	967:974	iso-C14 	967:974	Major fatty acids (>10 %) of cells were iso-C16 : 0, antiso-C15 : 0 and iso-C14 : 0.
33999796	8	44	dep	iso-C16 	935:942	arg1	 0					960:961	 0	960:961	 0	960:961	Major fatty acids (>10 %) of cells were iso-C16 : 0, antiso-C15 : 0 and iso-C14 : 0.
33999796	8	44	dep	iso-C16 	935:942	arg1	 0					944:945	 0	944:945	 0	944:945	Major fatty acids (>10 %) of cells were iso-C16 : 0, antiso-C15 : 0 and iso-C14 : 0.
33999796	8	44	dep	iso-C16 	935:942	arg1	 0					976:977	 0	976:977	 0	976:977	Major fatty acids (>10 %) of cells were iso-C16 : 0, antiso-C15 : 0 and iso-C14 : 0.
33999796	0	45	theme	gen.	19:22	arg1	nov.					24:27	Aquiluna borgnonia gen. nov.	0:27	Aquiluna borgnonia gen. nov.	0:27	Aquiluna borgnonia gen. nov., sp.
33999796	4	46	theme	single	524:529	arg1	genes					536:540	118 single copy genes	520:540	118 single copy genes referred strain 15G-AUS-rotT to the family Microbacteriaceae and therein to the so-called subcluster Luna-1	520:648	Phylogenetic reconstructions based on 16S rRNA gene sequences and genome-based on amino acid sequences of 118 single copy genes referred strain 15G-AUS-rotT to the family Microbacteriaceae and therein to the so-called subcluster Luna-1.
33999796	3	47	theme	rRNA	259:262	arg1	similarities					278:289	16S rRNA gene sequence similarities	255:289	16S rRNA gene sequence similarities of 98.7 % to Candidatus Aquiluna rubra and of 96.6 and 96.7 % to the two validly described species of the genus Rhodoluna	255:411	The strain showed 16S rRNA gene sequence similarities of 98.7 % to Candidatus Aquiluna rubra and of 96.6 and 96.7 % to the two validly described species of the genus Rhodoluna.
33999796	13	48	theme	new	1469:1471	arg1	genus					1473:1477	a new genus	1467:1477	a new genus	1467:1477	Based on the characteristics of the strain, a new genus and a new species termed Aquiluna borgnonia is proposed for strain 15G-AUS-rotT (=DSM 107803T=JCM 32974T).
33999796	9	49	theme	minor	1026:1030	arg1	MK-10					1046:1050	MK-10	1046:1050	MK-10	1046:1050	The major respiratory quinone was MK-11 and a minor component was MK-10.
33999796	9	49	theme	minor	1026:1030	arg1	component					1032:1040	a minor component	1024:1040	a minor component	1024:1040	The major respiratory quinone was MK-11 and a minor component was MK-10.
33999796	13	50	theme	Aquiluna	1504:1511	arg1	borgnonia					1513:1521	Aquiluna borgnonia	1504:1521	Aquiluna borgnonia	1504:1521	Based on the characteristics of the strain, a new genus and a new species termed Aquiluna borgnonia is proposed for strain 15G-AUS-rotT (=DSM 107803T=JCM 32974T).
33999796	12	51	theme	different	1388:1396	arg1	positions					1398:1406	three different positions	1382:1406	three different positions of the genome	1382:1420	An interesting feature was the presence of genes putatively encoding the complete light-driven proton pumping actinorhodopsin/retinal system, which were located at three different positions of the genome.
33999796	13	52	theme	new	1485:1487	arg1	species					1489:1495	a new species	1483:1495	a new species	1483:1495	Based on the characteristics of the strain, a new genus and a new species termed Aquiluna borgnonia is proposed for strain 15G-AUS-rotT (=DSM 107803T=JCM 32974T).
33999796	3	53	theme	genus	397:401	arg1	Rhodoluna					403:411	the genus Rhodoluna	393:411	the genus Rhodoluna	393:411	The strain showed 16S rRNA gene sequence similarities of 98.7 % to Candidatus Aquiluna rubra and of 96.6 and 96.7 % to the two validly described species of the genus Rhodoluna.
33999796	12	54	theme	actinorhodopsin/retinal	1328:1350	arg1	system					1352:1357	actinorhodopsin/retinal system	1328:1357	actinorhodopsin/retinal system	1328:1357	An interesting feature was the presence of genes putatively encoding the complete light-driven proton pumping actinorhodopsin/retinal system, which were located at three different positions of the genome.
33999796	3	55	dep	Candidatus	304:313	arg1	rubra					324:328	Candidatus Aquiluna rubra	304:328	Candidatus Aquiluna rubra	304:328	The strain showed 16S rRNA gene sequence similarities of 98.7 % to Candidatus Aquiluna rubra and of 96.6 and 96.7 % to the two validly described species of the genus Rhodoluna.
33999796	2	56	theme	strain	148:153	arg1	15G-AUS-rotT					155:166	The actinobacterial strain 15G-AUS-rotT	128:166	The actinobacterial strain 15G-AUS-rotT	128:166	The actinobacterial strain 15G-AUS-rotT was isolated from an artificial pond located near Salzburg, Austria.
33999796	10	57	theme	unusual	1096:1102	arg1	type					1106:1109	an unusual B type	1093:1109	an unusual B type	1093:1109	The peptidoglycan structure belonged to an unusual B type.
33999796	8	58	theme	fatty	901:905	arg1	acids					907:911	Major fatty acids	895:911	Major fatty acids (>10 %) of cells	895:928	Major fatty acids (>10 %) of cells were iso-C16 : 0, antiso-C15 : 0 and iso-C14 : 0.
33999796	8	58	theme	fatty	901:905	arg1	%					918:918	>10 %	914:918	>10 %	914:918	Major fatty acids (>10 %) of cells were iso-C16 : 0, antiso-C15 : 0 and iso-C14 : 0.
33999796	3	59	theme	Rhodoluna	403:411	arg1	species					382:388	the two validly described species	356:388	the two validly described species of the genus Rhodoluna	356:411	The strain showed 16S rRNA gene sequence similarities of 98.7 % to Candidatus Aquiluna rubra and of 96.6 and 96.7 % to the two validly described species of the genus Rhodoluna.
33999796	12	60	theme	genome	1415:1420	arg1	positions					1398:1406	three different positions	1382:1406	three different positions of the genome	1382:1420	An interesting feature was the presence of genes putatively encoding the complete light-driven proton pumping actinorhodopsin/retinal system, which were located at three different positions of the genome.
33999796	2	61	theme	actinobacterial	132:146	arg1	15G-AUS-rotT					155:166	The actinobacterial strain 15G-AUS-rotT	128:166	The actinobacterial strain 15G-AUS-rotT	128:166	The actinobacterial strain 15G-AUS-rotT was isolated from an artificial pond located near Salzburg, Austria.
33999796	0	62	dep	sp	30:31	arg1	nov.					24:27	Aquiluna borgnonia gen. nov.	0:27	Aquiluna borgnonia gen. nov.	0:27	Aquiluna borgnonia gen. nov., sp.
33999796	5	63	theme	genome-based	655:666	arg1	tree					681:684	The genome-based phylogenetic tree	651:684	The genome-based phylogenetic tree	651:684	The genome-based phylogenetic tree showed that the new strain represents a putative new genus.
33999796	1	64	theme	freshwater	83:92	arg1	bacteria					94:101	freshwater bacteria	83:101	freshwater bacteria	83:101	nov., a member of a Microbacteriaceae lineage of freshwater bacteria with small genome sizes.
33999796	13	65	theme	strain	1539:1544	arg1	15G-AUS-rotT					1546:1557	strain 15G-AUS-rotT	1539:1557	strain 15G-AUS-rotT (=DSM 107803T=JCM 32974T)	1539:1583	Based on the characteristics of the strain, a new genus and a new species termed Aquiluna borgnonia is proposed for strain 15G-AUS-rotT (=DSM 107803T=JCM 32974T).
33999796	13	65	theme	strain	1539:1544	arg1	32974T					1577:1582	=DSM 107803T=JCM 32974T	1560:1582	=DSM 107803T=JCM 32974T	1560:1582	Based on the characteristics of the strain, a new genus and a new species termed Aquiluna borgnonia is proposed for strain 15G-AUS-rotT (=DSM 107803T=JCM 32974T).
33999796	4	66	theme	acid	502:505	arg1	sequences					507:515	amino acid sequences	496:515	amino acid sequences of 118 single copy genes referred strain 15G-AUS-rotT to the family Microbacteriaceae and therein to the so-called subcluster Luna-1	496:648	Phylogenetic reconstructions based on 16S rRNA gene sequences and genome-based on amino acid sequences of 118 single copy genes referred strain 15G-AUS-rotT to the family Microbacteriaceae and therein to the so-called subcluster Luna-1.
33999796	6	67	theme	small	822:826	arg1	cells					840:844	very small, rod-shaped cells	817:844	very small, rod-shaped cells	817:844	Cultures of strain 15G-AUS-rotT were light red pigmented and comprised very small, rod-shaped cells.
33999796	12	68	theme	interesting	1221:1231	arg1	presence					1249:1256	the presence	1245:1256	the presence of genes putatively encoding the complete light-driven proton pumping actinorhodopsin/retinal system, which were located at three different positions of the genome	1245:1420	An interesting feature was the presence of genes putatively encoding the complete light-driven proton pumping actinorhodopsin/retinal system, which were located at three different positions of the genome.
33999796	12	68	theme	interesting	1221:1231	arg1	feature					1233:1239	An interesting feature	1218:1239	An interesting feature	1218:1239	An interesting feature was the presence of genes putatively encoding the complete light-driven proton pumping actinorhodopsin/retinal system, which were located at three different positions of the genome.
33999796	1	69	theme	bacteria	94:101	arg1	lineage					72:78	a Microbacteriaceae lineage	52:78	a Microbacteriaceae lineage of freshwater bacteria with small genome sizes	52:125	nov., a member of a Microbacteriaceae lineage of freshwater bacteria with small genome sizes.
33999796	7	70	theme	substrates	883:892	arg1	substrates					883:892	substrates	883:892	substrates	883:892	They metabolized a broad variety of substrates.
33999796	7	70	theme	substrates	883:892	arg1	variety					872:878	a broad variety	864:878	a broad variety of substrates	864:892	They metabolized a broad variety of substrates.
33999796	4	71	theme	amino	496:500	arg1	sequences					507:515	amino acid sequences	496:515	amino acid sequences of 118 single copy genes referred strain 15G-AUS-rotT to the family Microbacteriaceae and therein to the so-called subcluster Luna-1	496:648	Phylogenetic reconstructions based on 16S rRNA gene sequences and genome-based on amino acid sequences of 118 single copy genes referred strain 15G-AUS-rotT to the family Microbacteriaceae and therein to the so-called subcluster Luna-1.
33999796	3	72	theme	gene	264:267	arg1	similarities					278:289	16S rRNA gene sequence similarities	255:289	16S rRNA gene sequence similarities of 98.7 % to Candidatus Aquiluna rubra and of 96.6 and 96.7 % to the two validly described species of the genus Rhodoluna	255:411	The strain showed 16S rRNA gene sequence similarities of 98.7 % to Candidatus Aquiluna rubra and of 96.6 and 96.7 % to the two validly described species of the genus Rhodoluna.
33999796	6	73	dep	small	822:826	arg1	rod-shaped					829:838	rod-shaped	829:838	rod-shaped	829:838	Cultures of strain 15G-AUS-rotT were light red pigmented and comprised very small, rod-shaped cells.
33999796	3	74	theme	sequence	269:276	arg1	similarities					278:289	16S rRNA gene sequence similarities	255:289	16S rRNA gene sequence similarities of 98.7 % to Candidatus Aquiluna rubra and of 96.6 and 96.7 % to the two validly described species of the genus Rhodoluna	255:411	The strain showed 16S rRNA gene sequence similarities of 98.7 % to Candidatus Aquiluna rubra and of 96.6 and 96.7 % to the two validly described species of the genus Rhodoluna.
33999796	1	75	theme	small	108:112	arg1	sizes					121:125	small genome sizes	108:125	small genome sizes	108:125	nov., a member of a Microbacteriaceae lineage of freshwater bacteria with small genome sizes.
33999796	10	76	theme	B	1104:1104	arg1	type					1106:1109	an unusual B type	1093:1109	an unusual B type	1093:1109	The peptidoglycan structure belonged to an unusual B type.
33999796	5	77	theme	putative	726:733	arg1	genus					739:743	a putative new genus	724:743	a putative new genus	724:743	The genome-based phylogenetic tree showed that the new strain represents a putative new genus.
33999796	1	78	theme	genome	114:119	arg1	sizes					121:125	small genome sizes	108:125	small genome sizes	108:125	nov., a member of a Microbacteriaceae lineage of freshwater bacteria with small genome sizes.
33999796	4	79	theme	family	578:583	arg1	Microbacteriaceae					585:601	the family Microbacteriaceae	574:601	the family Microbacteriaceae	574:601	Phylogenetic reconstructions based on 16S rRNA gene sequences and genome-based on amino acid sequences of 118 single copy genes referred strain 15G-AUS-rotT to the family Microbacteriaceae and therein to the so-called subcluster Luna-1.
33999796	5	80	theme	new	735:737	arg1	genus					739:743	a putative new genus	724:743	a putative new genus	724:743	The genome-based phylogenetic tree showed that the new strain represents a putative new genus.
33999796	4	81	theme	gene	461:464	arg1	sequences					466:474	16S rRNA gene sequences	452:474	16S rRNA gene sequences	452:474	Phylogenetic reconstructions based on 16S rRNA gene sequences and genome-based on amino acid sequences of 118 single copy genes referred strain 15G-AUS-rotT to the family Microbacteriaceae and therein to the so-called subcluster Luna-1.
33999796	1	82	with	lineage	72:78	arg1	sizes					121:125	small genome sizes	108:125	small genome sizes	108:125	nov., a member of a Microbacteriaceae lineage of freshwater bacteria with small genome sizes.
33064070	3	0	theme	short	319:323	arg1	rods					325:328	short rods	319:328	short rods	319:328	Cells were short rods.
33064070	7	1	theme	G+C	622:624	arg1	content					626:632	The genomic DNA G+C content	606:632	The genomic DNA G+C content of strain CBS5P-1T	606:651	The genomic DNA G+C content of strain CBS5P-1T was 71.8 mol%.
33064070	7	1	theme	G+C	622:624	arg1	%					665:665	71.8 mol%	657:665	71.8 mol%	657:665	The genomic DNA G+C content of strain CBS5P-1T was 71.8 mol%.
33064070	13	2	theme	new	1170:1172	arg1	member					1174:1179	this new member	1165:1179	this new member of the genus Microbacterium	1165:1207	nov. is proposed to accommodate this new member of the genus Microbacterium.
33064070	6	3	theme	phylogenetic	493:504	arg1	neighbour					506:514	Its nearest phylogenetic neighbour	481:514	Its nearest phylogenetic neighbour	481:514	Its nearest phylogenetic neighbour was Microbacterium amylolyticum DSM 24221T with 97.1 % 16S rRNA gene sequence similarity.
33064070	6	4	theme	sequence	585:592	arg1	similarity					594:603	97.1 % 16S rRNA gene sequence similarity	564:603	97.1 % 16S rRNA gene sequence similarity	564:603	Its nearest phylogenetic neighbour was Microbacterium amylolyticum DSM 24221T with 97.1 % 16S rRNA gene sequence similarity.
33064070	6	5	theme	nearest	485:491	arg1	neighbour					506:514	Its nearest phylogenetic neighbour	481:514	Its nearest phylogenetic neighbour	481:514	Its nearest phylogenetic neighbour was Microbacterium amylolyticum DSM 24221T with 97.1 % 16S rRNA gene sequence similarity.
33064070	7	6	theme	genomic	610:616	arg1	content					626:632	The genomic DNA G+C content	606:632	The genomic DNA G+C content of strain CBS5P-1T	606:651	The genomic DNA G+C content of strain CBS5P-1T was 71.8 mol%.
33064070	7	6	theme	genomic	610:616	arg1	%					665:665	71.8 mol%	657:665	71.8 mol%	657:665	The genomic DNA G+C content of strain CBS5P-1T was 71.8 mol%.
33064070	2	7	theme	PR	298:299	arg1	Region					290:295	Guangxi Zhuang Autonomous Region	264:295	Guangxi Zhuang Autonomous Region	264:295	A Gram-stain-positive, aerobic actinobacterium, designated strain CBS5P-1T, was isolated from bark of Excoecaria agallocha Linn collected from Guangxi Zhuang Autonomous Region, PR China.
33064070	2	7	theme	PR	298:299	arg1	China					301:305	PR China	298:305	PR China	298:305	A Gram-stain-positive, aerobic actinobacterium, designated strain CBS5P-1T, was isolated from bark of Excoecaria agallocha Linn collected from Guangxi Zhuang Autonomous Region, PR China.
33064070	6	8	theme	gene	580:583	arg1	similarity					594:603	97.1 % 16S rRNA gene sequence similarity	564:603	97.1 % 16S rRNA gene sequence similarity	564:603	Its nearest phylogenetic neighbour was Microbacterium amylolyticum DSM 24221T with 97.1 % 16S rRNA gene sequence similarity.
33064070	8	9	theme	cellular	741:748	arg1	acids					756:760	predominant cellular fatty acids	729:760	predominant cellular fatty acids	729:760	Anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and C16:0 were predominant cellular fatty acids.
33064070	13	10	theme	Microbacterium	1194:1207	arg1	member					1174:1179	this new member	1165:1179	this new member of the genus Microbacterium	1165:1207	nov. is proposed to accommodate this new member of the genus Microbacterium.
33064070	9	11	theme	Major	763:767	arg1	menaquinones					769:780	Major menaquinones	763:780	Major menaquinones	763:780	Major menaquinones were MK-11 and MK-10.
33064070	8	12	theme	predominant	729:739	arg1	acids					756:760	predominant cellular fatty acids	729:760	predominant cellular fatty acids	729:760	Anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and C16:0 were predominant cellular fatty acids.
33064070	11	13	theme	phenotypic	993:1002	arg1	data					1004:1007	chemotaxonomic, phylogenetic and phenotypic data	960:1007	chemotaxonomic, phylogenetic and phenotypic data	960:1007	The combination of chemotaxonomic, phylogenetic and phenotypic data clearly distinguished strain CBS5P-1T from its phylogenetic neighbour.
33064070	10	14	theme	polar	808:812	arg1	lipids					814:819	The polar lipids	804:819	The polar lipids	804:819	The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid and an unidentified phospholipid.
33064070	5	15	dep	optimum	426:432	arg1	30 °C					435:439	30 °C	435:439	30 °C	435:439	Strain CBS5P-1T grew at 10-37 °C (optimum, 30 °C) and pH 6.0-12.0 (optimum, pH 7.0-8.0).
33064070	11	16	theme	phylogenetic	1056:1067	arg1	neighbour					1069:1077	its phylogenetic neighbour	1052:1077	its phylogenetic neighbour	1052:1077	The combination of chemotaxonomic, phylogenetic and phenotypic data clearly distinguished strain CBS5P-1T from its phylogenetic neighbour.
33064070	12	17	theme	excoecariae	1117:1127	arg1	sp					1129:1130	the name Microbacterium excoecariae sp	1093:1130	the name Microbacterium excoecariae sp	1093:1130	Accordingly, the name Microbacterium excoecariae sp.
33064070	2	18	theme	Autonomous	279:288	arg1	Region					290:295	Guangxi Zhuang Autonomous Region	264:295	Guangxi Zhuang Autonomous Region	264:295	A Gram-stain-positive, aerobic actinobacterium, designated strain CBS5P-1T, was isolated from bark of Excoecaria agallocha Linn collected from Guangxi Zhuang Autonomous Region, PR China.
33064070	2	18	theme	Autonomous	279:288	arg1	China					301:305	PR China	298:305	PR China	298:305	A Gram-stain-positive, aerobic actinobacterium, designated strain CBS5P-1T, was isolated from bark of Excoecaria agallocha Linn collected from Guangxi Zhuang Autonomous Region, PR China.
33064070	5	19	theme	pH	468:469	arg1	7.0-8.0					471:477	pH 7.0-8.0	468:477	pH 7.0-8.0	468:477	Strain CBS5P-1T grew at 10-37 °C (optimum, 30 °C) and pH 6.0-12.0 (optimum, pH 7.0-8.0).
33064070	4	20	contain	had	372:374	arg1	Colonies					331:338	Colonies	331:338	Colonies	331:338	Colonies were light yellow, circular and had entire margins.
33064070	4	20	contain	had	372:374	arg2	margins					383:389	entire margins	376:389	entire margins	376:389	Colonies were light yellow, circular and had entire margins.
33064070	14	21	theme	49239T=CGMCC	1245:1256	arg1	1.13862T					1258:1265	=KCTC 49239T=CGMCC 1.13862T	1239:1265	=KCTC 49239T=CGMCC 1.13862T	1239:1265	The type strain is CBS5P-1T (=KCTC 49239T=CGMCC 1.13862T).
33064070	14	21	theme	49239T=CGMCC	1245:1256	arg1	CBS5P-1T					1229:1236	CBS5P-1T	1229:1236	CBS5P-1T (=KCTC 49239T=CGMCC 1.13862T)	1229:1266	The type strain is CBS5P-1T (=KCTC 49239T=CGMCC 1.13862T).
33064070	0	22	theme	Microbacterium	0:13	arg1	sp					27:28	Microbacterium excoecariae sp	0:28	Microbacterium excoecariae sp.	0:29	Microbacterium excoecariae sp.
33064070	14	23	theme	=KCTC	1239:1243	arg1	1.13862T					1258:1265	=KCTC 49239T=CGMCC 1.13862T	1239:1265	=KCTC 49239T=CGMCC 1.13862T	1239:1265	The type strain is CBS5P-1T (=KCTC 49239T=CGMCC 1.13862T).
33064070	14	23	theme	=KCTC	1239:1243	arg1	CBS5P-1T					1229:1236	CBS5P-1T	1229:1236	CBS5P-1T (=KCTC 49239T=CGMCC 1.13862T)	1229:1266	The type strain is CBS5P-1T (=KCTC 49239T=CGMCC 1.13862T).
33064070	4	24	theme	entire	376:381	arg1	margins					383:389	entire margins	376:389	entire margins	376:389	Colonies were light yellow, circular and had entire margins.
33064070	2	25	attach	isolated	201:208	arg2	actinobacterium					152:166	A Gram-stain-positive, aerobic actinobacterium	121:166	A Gram-stain-positive, aerobic actinobacterium	121:166	A Gram-stain-positive, aerobic actinobacterium, designated strain CBS5P-1T, was isolated from bark of Excoecaria agallocha Linn collected from Guangxi Zhuang Autonomous Region, PR China.
33064070	2	25	attach	isolated	201:208	arg1	bark					215:218	bark	215:218	bark of Excoecaria agallocha Linn collected from Guangxi Zhuang Autonomous Region, PR China	215:305	A Gram-stain-positive, aerobic actinobacterium, designated strain CBS5P-1T, was isolated from bark of Excoecaria agallocha Linn collected from Guangxi Zhuang Autonomous Region, PR China.
33064070	6	26	theme	%	569:569	arg1	similarity					594:603	97.1 % 16S rRNA gene sequence similarity	564:603	97.1 % 16S rRNA gene sequence similarity	564:603	Its nearest phylogenetic neighbour was Microbacterium amylolyticum DSM 24221T with 97.1 % 16S rRNA gene sequence similarity.
33064070	6	27	with	24221T	552:557	arg1	similarity					594:603	97.1 % 16S rRNA gene sequence similarity	564:603	97.1 % 16S rRNA gene sequence similarity	564:603	Its nearest phylogenetic neighbour was Microbacterium amylolyticum DSM 24221T with 97.1 % 16S rRNA gene sequence similarity.
33064070	4	28	dep	yellow	351:356	arg1	circular					359:366	circular	359:366	circular	359:366	Colonies were light yellow, circular and had entire margins.
33064070	6	29	theme	97.1 	564:568	arg1	%					569:569	%	569:569	%	569:569	Its nearest phylogenetic neighbour was Microbacterium amylolyticum DSM 24221T with 97.1 % 16S rRNA gene sequence similarity.
33064070	10	30	theme	unidentified	883:894	arg1	glycolipid					896:905	an unidentified glycolipid	880:905	an unidentified glycolipid	880:905	The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid and an unidentified phospholipid.
33064070	12	31	theme	Microbacterium	1102:1115	arg1	sp					1129:1130	the name Microbacterium excoecariae sp	1093:1130	the name Microbacterium excoecariae sp	1093:1130	Accordingly, the name Microbacterium excoecariae sp.
33064070	1	32	attach	isolated	72:79	arg1	bark					86:89	bark	86:89	bark of Excoecaria agallocha Linn	86:118	nov., a novel endophytic actinobacterium isolated from bark of Excoecaria agallocha Linn.
33064070	1	32	attach	isolated	72:79	arg2	actinobacterium					56:70	a novel endophytic actinobacterium	37:70	a novel endophytic actinobacterium isolated from bark of Excoecaria agallocha Linn	37:118	nov., a novel endophytic actinobacterium isolated from bark of Excoecaria agallocha Linn.
33064070	2	33	theme	Linn	244:247	arg1	bark					215:218	bark	215:218	bark of Excoecaria agallocha Linn collected from Guangxi Zhuang Autonomous Region, PR China	215:305	A Gram-stain-positive, aerobic actinobacterium, designated strain CBS5P-1T, was isolated from bark of Excoecaria agallocha Linn collected from Guangxi Zhuang Autonomous Region, PR China.
33064070	12	34	theme	name	1097:1100	arg1	sp					1129:1130	the name Microbacterium excoecariae sp	1093:1130	the name Microbacterium excoecariae sp	1093:1130	Accordingly, the name Microbacterium excoecariae sp.
33064070	11	35	theme	chemotaxonomic	960:973	arg1	data					1004:1007	chemotaxonomic, phylogenetic and phenotypic data	960:1007	chemotaxonomic, phylogenetic and phenotypic data	960:1007	The combination of chemotaxonomic, phylogenetic and phenotypic data clearly distinguished strain CBS5P-1T from its phylogenetic neighbour.
33064070	2	36	theme	strain	180:185	arg1	CBS5P-1T					187:194	strain CBS5P-1T	180:194	strain CBS5P-1T	180:194	A Gram-stain-positive, aerobic actinobacterium, designated strain CBS5P-1T, was isolated from bark of Excoecaria agallocha Linn collected from Guangxi Zhuang Autonomous Region, PR China.
33064070	6	37	theme	DSM	548:550	arg1	24221T					552:557	Microbacterium amylolyticum DSM 24221T	520:557	Microbacterium amylolyticum DSM 24221T with 97.1 % 16S rRNA gene sequence similarity	520:603	Its nearest phylogenetic neighbour was Microbacterium amylolyticum DSM 24221T with 97.1 % 16S rRNA gene sequence similarity.
33064070	14	38	theme	type	1214:1217	arg1	CBS5P-1T					1229:1236	CBS5P-1T	1229:1236	CBS5P-1T (=KCTC 49239T=CGMCC 1.13862T)	1229:1266	The type strain is CBS5P-1T (=KCTC 49239T=CGMCC 1.13862T).
33064070	14	38	theme	type	1214:1217	arg1	strain					1219:1224	The type strain	1210:1224	The type strain	1210:1224	The type strain is CBS5P-1T (=KCTC 49239T=CGMCC 1.13862T).
33064070	7	39	theme	71.8 mol	657:664	arg1	content					626:632	The genomic DNA G+C content	606:632	The genomic DNA G+C content of strain CBS5P-1T	606:651	The genomic DNA G+C content of strain CBS5P-1T was 71.8 mol%.
33064070	7	39	theme	71.8 mol	657:664	arg1	%					665:665	71.8 mol%	657:665	71.8 mol%	657:665	The genomic DNA G+C content of strain CBS5P-1T was 71.8 mol%.
33064070	1	40	theme	Linn	115:118	arg1	bark					86:89	bark	86:89	bark of Excoecaria agallocha Linn	86:118	nov., a novel endophytic actinobacterium isolated from bark of Excoecaria agallocha Linn.
33064070	6	41	theme	amylolyticum	535:546	arg1	24221T					552:557	Microbacterium amylolyticum DSM 24221T	520:557	Microbacterium amylolyticum DSM 24221T with 97.1 % 16S rRNA gene sequence similarity	520:603	Its nearest phylogenetic neighbour was Microbacterium amylolyticum DSM 24221T with 97.1 % 16S rRNA gene sequence similarity.
33064070	2	42	dep	Gram-stain-positive	123:141	arg1	aerobic					144:150	aerobic	144:150	aerobic	144:150	A Gram-stain-positive, aerobic actinobacterium, designated strain CBS5P-1T, was isolated from bark of Excoecaria agallocha Linn collected from Guangxi Zhuang Autonomous Region, PR China.
33064070	7	43	theme	DNA	618:620	arg1	content					626:632	The genomic DNA G+C content	606:632	The genomic DNA G+C content of strain CBS5P-1T	606:651	The genomic DNA G+C content of strain CBS5P-1T was 71.8 mol%.
33064070	7	43	theme	DNA	618:620	arg1	%					665:665	71.8 mol%	657:665	71.8 mol%	657:665	The genomic DNA G+C content of strain CBS5P-1T was 71.8 mol%.
33064070	7	44	theme	strain	637:642	arg1	CBS5P-1T					644:651	strain CBS5P-1T	637:651	strain CBS5P-1T	637:651	The genomic DNA G+C content of strain CBS5P-1T was 71.8 mol%.
33064070	10	45	theme	unidentified	914:925	arg1	phospholipid					927:938	an unidentified phospholipid	911:938	an unidentified phospholipid	911:938	The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid and an unidentified phospholipid.
33064070	5	46	dep	optimum	459:465	arg1	7.0-8.0					471:477	pH 7.0-8.0	468:477	pH 7.0-8.0	468:477	Strain CBS5P-1T grew at 10-37 °C (optimum, 30 °C) and pH 6.0-12.0 (optimum, pH 7.0-8.0).
33064070	11	47	theme	data	1004:1007	arg1	combination					945:955	The combination	941:955	The combination of chemotaxonomic, phylogenetic and phenotypic data	941:1007	The combination of chemotaxonomic, phylogenetic and phenotypic data clearly distinguished strain CBS5P-1T from its phylogenetic neighbour.
33064070	7	48	theme	CBS5P-1T	644:651	arg1	content					626:632	The genomic DNA G+C content	606:632	The genomic DNA G+C content of strain CBS5P-1T	606:651	The genomic DNA G+C content of strain CBS5P-1T was 71.8 mol%.
33064070	7	48	theme	CBS5P-1T	644:651	arg1	%					665:665	71.8 mol%	657:665	71.8 mol%	657:665	The genomic DNA G+C content of strain CBS5P-1T was 71.8 mol%.
33064070	2	49	theme	Zhuang	272:277	arg1	Region					290:295	Guangxi Zhuang Autonomous Region	264:295	Guangxi Zhuang Autonomous Region	264:295	A Gram-stain-positive, aerobic actinobacterium, designated strain CBS5P-1T, was isolated from bark of Excoecaria agallocha Linn collected from Guangxi Zhuang Autonomous Region, PR China.
33064070	2	49	theme	Zhuang	272:277	arg1	China					301:305	PR China	298:305	PR China	298:305	A Gram-stain-positive, aerobic actinobacterium, designated strain CBS5P-1T, was isolated from bark of Excoecaria agallocha Linn collected from Guangxi Zhuang Autonomous Region, PR China.
33064070	13	50	theme	genus	1188:1192	arg1	Microbacterium					1194:1207	the genus Microbacterium	1184:1207	the genus Microbacterium	1184:1207	nov. is proposed to accommodate this new member of the genus Microbacterium.
33064070	11	51	theme	phylogenetic	976:987	arg1	data					1004:1007	chemotaxonomic, phylogenetic and phenotypic data	960:1007	chemotaxonomic, phylogenetic and phenotypic data	960:1007	The combination of chemotaxonomic, phylogenetic and phenotypic data clearly distinguished strain CBS5P-1T from its phylogenetic neighbour.
33064070	8	52	theme	fatty	750:754	arg1	acids					756:760	predominant cellular fatty acids	729:760	predominant cellular fatty acids	729:760	Anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and C16:0 were predominant cellular fatty acids.
33064070	1	53	theme	endophytic	45:54	arg1	actinobacterium					56:70	a novel endophytic actinobacterium	37:70	a novel endophytic actinobacterium isolated from bark of Excoecaria agallocha Linn	37:118	nov., a novel endophytic actinobacterium isolated from bark of Excoecaria agallocha Linn.
33064070	1	54	dep	actinobacterium	56:70	arg1	nov.					31:34	nov.	31:34	nov.	31:34	nov., a novel endophytic actinobacterium isolated from bark of Excoecaria agallocha Linn.
33064070	6	55	theme	Microbacterium	520:533	arg1	24221T					552:557	Microbacterium amylolyticum DSM 24221T	520:557	Microbacterium amylolyticum DSM 24221T with 97.1 % 16S rRNA gene sequence similarity	520:603	Its nearest phylogenetic neighbour was Microbacterium amylolyticum DSM 24221T with 97.1 % 16S rRNA gene sequence similarity.
33064070	8	56	dep	Anteiso-C15	668:678	arg1	 0					681:682	 0	681:682	 0	681:682	Anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and C16:0 were predominant cellular fatty acids.
33064070	8	56	dep	Anteiso-C15	668:678	arg1	 0					711:712	 0	711:712	 0	711:712	Anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and C16:0 were predominant cellular fatty acids.
33064070	8	56	dep	Anteiso-C15	668:678	arg1	 0					698:699	 0	698:699	 0	698:699	Anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and C16:0 were predominant cellular fatty acids.
33064070	8	56	dep	Anteiso-C15	668:678	arg1	C16:0					718:722	C16:0	718:722	C16:0	718:722	Anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and C16:0 were predominant cellular fatty acids.
33064070	6	57	theme	16S	571:573	arg1	similarity					594:603	97.1 % 16S rRNA gene sequence similarity	564:603	97.1 % 16S rRNA gene sequence similarity	564:603	Its nearest phylogenetic neighbour was Microbacterium amylolyticum DSM 24221T with 97.1 % 16S rRNA gene sequence similarity.
33064070	11	58	theme	strain	1031:1036	arg1	CBS5P-1T					1038:1045	strain CBS5P-1T	1031:1045	strain CBS5P-1T	1031:1045	The combination of chemotaxonomic, phylogenetic and phenotypic data clearly distinguished strain CBS5P-1T from its phylogenetic neighbour.
33064070	6	59	theme	rRNA	575:578	arg1	similarity					594:603	97.1 % 16S rRNA gene sequence similarity	564:603	97.1 % 16S rRNA gene sequence similarity	564:603	Its nearest phylogenetic neighbour was Microbacterium amylolyticum DSM 24221T with 97.1 % 16S rRNA gene sequence similarity.
33064070	2	60	theme	Gram-stain-positive	123:141	arg1	actinobacterium					152:166	A Gram-stain-positive, aerobic actinobacterium	121:166	A Gram-stain-positive, aerobic actinobacterium	121:166	A Gram-stain-positive, aerobic actinobacterium, designated strain CBS5P-1T, was isolated from bark of Excoecaria agallocha Linn collected from Guangxi Zhuang Autonomous Region, PR China.
33064070	1	61	theme	novel	39:43	arg1	actinobacterium					56:70	a novel endophytic actinobacterium	37:70	a novel endophytic actinobacterium isolated from bark of Excoecaria agallocha Linn	37:118	nov., a novel endophytic actinobacterium isolated from bark of Excoecaria agallocha Linn.
33064070	0	62	theme	excoecariae	15:25	arg1	sp					27:28	Microbacterium excoecariae sp	0:28	Microbacterium excoecariae sp.	0:29	Microbacterium excoecariae sp.
32376684	4	0	from	sequences	715:723	arg1	EOGT					728:731	EOGT	728:731	EOGT	728:731	Three predicted N-glycosylation consensus sequences on EOGT are highly conserved among mammalian species.
32376684	1	1	theme	-resident	245:253	arg1	transferase					194:204	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase	139:204	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT)	139:211	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT) is an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling.
32376684	1	1	theme	-resident	245:253	arg1	protein					255:261	an endoplasmic reticulum (ER)-resident protein	216:261	an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling	216:371	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT) is an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling.
32376684	7	2	theme	enzyme	1111:1116	arg1	activity					1118:1125	enzyme activity	1111:1125	enzyme activity	1111:1125	Loss of an individual N-glycan on EOGT did not affect its endoplasmic reticulum (ER) localization, enzyme activity, and ability to O-GlcNAcylate Notch1 in HEK293T cells.
32376684	6	3	theme	endoglycosidase	897:911	arg1	digestion					915:923	endoglycosidase H digestion	897:923	endoglycosidase H digestion	897:923	Lectin blotting, endoglycosidase H digestion, and MS analysis revealed that both residues are modified with oligomannose N-glycans.
32376684	1	4	theme	O-GlcNAc	185:192	arg1	transferase					194:204	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase	139:204	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT)	139:211	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT) is an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling.
32376684	1	4	theme	O-GlcNAc	185:192	arg1	protein					255:261	an endoplasmic reticulum (ER)-resident protein	216:261	an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling	216:371	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT) is an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling.
32376684	1	4	theme	O-GlcNAc	185:192	arg1	EOGT					207:210	EOGT	207:210	EOGT	207:210	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT) is an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling.
32376684	9	5	located	observed	1502:1509	arg1	Notch1					1514:1519	Notch1	1514:1519	Notch1	1514:1519	Accordingly, a decrease in O-GlcNAc stoichiometry was observed in Notch1 co-expressed with an N263Q/N354Q variant compared with WT EOGT.
32376684	9	5	located	observed	1502:1509	arg2	decrease					1463:1470	a decrease	1461:1470	a decrease in O-GlcNAc stoichiometry	1461:1496	Accordingly, a decrease in O-GlcNAc stoichiometry was observed in Notch1 co-expressed with an N263Q/N354Q variant compared with WT EOGT.
32376684	8	6	theme	EOGT	1261:1264	arg1	maturation					1266:1275	EOGT maturation	1261:1275	EOGT maturation	1261:1275	However, simultaneous substitution of both N-glycosylation sites affected both EOGT maturation and expression levels without an apparent change in enzymatic activity, suggesting that N-glycosylation at a single site is sufficient for EOGT maturation and expression.
32376684	10	7	theme	altered	1629:1635	arg1	distribution					1649:1660	altered subcellular distribution	1629:1660	altered subcellular distribution within the ER in HEK293T cells	1629:1691	Moreover, the N263Q/N354Q variant exhibited altered subcellular distribution within the ER in HEK293T cells, indicating that N-glycosylation of EOGT is required for its ER localization at the cell periphery.
32376684	11	8	theme	N-glycans	1833:1841	arg1	roles					1824:1828	critical roles	1815:1828	critical roles of N-glycans	1815:1841	These results suggest critical roles of N-glycans in sustaining O-GlcNAc transferase function both by maintaining EOGT levels and by ensuring its proper subcellular localization in the ER.
32376684	8	9	theme	simultaneous	1191:1202	arg1	substitution					1204:1215	simultaneous substitution	1191:1215	simultaneous substitution of both N-glycosylation sites	1191:1245	However, simultaneous substitution of both N-glycosylation sites affected both EOGT maturation and expression levels without an apparent change in enzymatic activity, suggesting that N-glycosylation at a single site is sufficient for EOGT maturation and expression.
32376684	7	10	theme	reticulum	1082:1090	arg1	localization					1097:1108	its endoplasmic reticulum (ER) localization	1066:1108	its endoplasmic reticulum (ER) localization	1066:1108	Loss of an individual N-glycan on EOGT did not affect its endoplasmic reticulum (ER) localization, enzyme activity, and ability to O-GlcNAcylate Notch1 in HEK293T cells.
32376684	10	11	gly	N-glycosylation	1710:1724	arg1	ER					1754:1755	its ER localization	1750:1768	its ER localization at the cell periphery	1750:1790	Moreover, the N263Q/N354Q variant exhibited altered subcellular distribution within the ER in HEK293T cells, indicating that N-glycosylation of EOGT is required for its ER localization at the cell periphery.
32376684	10	11	gly	N-glycosylation	1710:1724	arg1	EOGT					1729:1732	EOGT	1729:1732	EOGT	1729:1732	Moreover, the N263Q/N354Q variant exhibited altered subcellular distribution within the ER in HEK293T cells, indicating that N-glycosylation of EOGT is required for its ER localization at the cell periphery.
32376684	11	12	theme	transferase	1866:1876	arg1	function					1878:1885	O-GlcNAc transferase function	1857:1885	O-GlcNAc transferase function	1857:1885	These results suggest critical roles of N-glycans in sustaining O-GlcNAc transferase function both by maintaining EOGT levels and by ensuring its proper subcellular localization in the ER.
32376684	7	13	theme	individual	1023:1032	arg1	N-glycan					1034:1041	an individual N-glycan	1020:1041	an individual N-glycan	1020:1041	Loss of an individual N-glycan on EOGT did not affect its endoplasmic reticulum (ER) localization, enzyme activity, and ability to O-GlcNAcylate Notch1 in HEK293T cells.
32376684	8	14	theme	N-glycosylation	1225:1239	arg1	sites					1241:1245	both N-glycosylation sites	1220:1245	both N-glycosylation sites	1220:1245	However, simultaneous substitution of both N-glycosylation sites affected both EOGT maturation and expression levels without an apparent change in enzymatic activity, suggesting that N-glycosylation at a single site is sufficient for EOGT maturation and expression.
32376684	11	15	dep	suggest	1807:1813	arg1	maintaining					1895:1905	maintaining	1895:1905	maintaining EOGT levels	1895:1917	These results suggest critical roles of N-glycans in sustaining O-GlcNAc transferase function both by maintaining EOGT levels and by ensuring its proper subcellular localization in the ER.
32376684	11	15	dep	suggest	1807:1813	arg1	ensuring					1926:1933	ensuring	1926:1933	ensuring its proper subcellular localization in the ER	1926:1979	These results suggest critical roles of N-glycans in sustaining O-GlcNAc transferase function both by maintaining EOGT levels and by ensuring its proper subcellular localization in the ER.
32376684	1	16	theme	growth	149:154	arg1	EGF					164:166	EGF	164:166	EGF	164:166	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT) is an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling.
32376684	1	16	theme	growth	149:154	arg1	factor					156:161	Epidermal growth factor	139:161	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT)	139:211	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT) is an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling.
32376684	8	17	theme	enzymatic	1329:1337	arg1	activity					1339:1346	enzymatic activity	1329:1346	enzymatic activity	1329:1346	However, simultaneous substitution of both N-glycosylation sites affected both EOGT maturation and expression levels without an apparent change in enzymatic activity, suggesting that N-glycosylation at a single site is sufficient for EOGT maturation and expression.
32376684	3	18	theme	mouse	620:624	arg1	EOGT					626:629	mouse EOGT	620:629	mouse EOGT	620:629	Here, we identified N-glycosylation sites in mouse EOGT and elucidated their molecular functions.
32376684	9	19	theme	N263Q/N354Q	1542:1552	arg1	variant					1554:1560	an N263Q/N354Q variant	1539:1560	an N263Q/N354Q variant	1539:1560	Accordingly, a decrease in O-GlcNAc stoichiometry was observed in Notch1 co-expressed with an N263Q/N354Q variant compared with WT EOGT.
32376684	7	20	from	ability	1132:1138	arg1	cells					1175:1179	HEK293T cells	1167:1179	HEK293T cells	1167:1179	Loss of an individual N-glycan on EOGT did not affect its endoplasmic reticulum (ER) localization, enzyme activity, and ability to O-GlcNAcylate Notch1 in HEK293T cells.
32376684	0	21	theme	EOGT	72:75	arg1	maturation					77:86	EOGT maturation	72:86	EOGT maturation	72:86	N-Glycans on EGF domain-specific O-GlcNAc transferase (EOGT) facilitate EOGT maturation and peripheral endoplasmic reticulum localization.
32376684	1	22	theme	ligand-mediated	341:355	arg1	signaling					363:371	Delta-like ligand-mediated Notch signaling	330:371	Delta-like ligand-mediated Notch signaling	330:371	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT) is an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling.
32376684	8	23	from	change	1319:1324	arg1	activity					1339:1346	enzymatic activity	1329:1346	enzymatic activity	1329:1346	However, simultaneous substitution of both N-glycosylation sites affected both EOGT maturation and expression levels without an apparent change in enzymatic activity, suggesting that N-glycosylation at a single site is sufficient for EOGT maturation and expression.
32376684	0	24	from	N-Glycans	0:8	arg1	transferase					42:52	EGF domain-specific O-GlcNAc transferase	13:52	EGF domain-specific O-GlcNAc transferase (EOGT)	13:59	N-Glycans on EGF domain-specific O-GlcNAc transferase (EOGT) facilitate EOGT maturation and peripheral endoplasmic reticulum localization.
32376684	0	24	from	N-Glycans	0:8	arg1	EOGT					55:58	EOGT	55:58	EOGT	55:58	N-Glycans on EGF domain-specific O-GlcNAc transferase (EOGT) facilitate EOGT maturation and peripheral endoplasmic reticulum localization.
32376684	6	25	theme	oligomannose	988:999	arg1	N-glycans					1001:1009	oligomannose N-glycans	988:1009	oligomannose N-glycans	988:1009	Lectin blotting, endoglycosidase H digestion, and MS analysis revealed that both residues are modified with oligomannose N-glycans.
32376684	2	26	theme	N-glycans	524:532	arg1	function					512:519	function	512:519	function	512:519	Several EOGT mutations that may affect putative N-glycosylation consensus sites are recorded in the cancer database, but the presence and function of N-glycans in EOGT have not yet been characterized.
32376684	2	26	theme	N-glycans	524:532	arg1	presence					499:506	presence	499:506	presence	499:506	Several EOGT mutations that may affect putative N-glycosylation consensus sites are recorded in the cancer database, but the presence and function of N-glycans in EOGT have not yet been characterized.
32376684	11	27	theme	subcellular	1946:1956	arg1	localization					1958:1969	its proper subcellular localization	1935:1969	its proper subcellular localization	1935:1969	These results suggest critical roles of N-glycans in sustaining O-GlcNAc transferase function both by maintaining EOGT levels and by ensuring its proper subcellular localization in the ER.
32376684	0	28	theme	endoplasmic	103:113	arg1	reticulum					115:123	peripheral endoplasmic reticulum	92:123	peripheral endoplasmic reticulum localization	92:136	N-Glycans on EGF domain-specific O-GlcNAc transferase (EOGT) facilitate EOGT maturation and peripheral endoplasmic reticulum localization.
32376684	8	29	theme	single	1386:1391	arg1	site					1393:1396	a single site	1384:1396	a single site	1384:1396	However, simultaneous substitution of both N-glycosylation sites affected both EOGT maturation and expression levels without an apparent change in enzymatic activity, suggesting that N-glycosylation at a single site is sufficient for EOGT maturation and expression.
32376684	10	30	from	distribution	1649:1660	arg1	cells					1687:1691	HEK293T cells	1679:1691	HEK293T cells	1679:1691	Moreover, the N263Q/N354Q variant exhibited altered subcellular distribution within the ER in HEK293T cells, indicating that N-glycosylation of EOGT is required for its ER localization at the cell periphery.
32376684	10	31	theme	HEK293T	1679:1685	arg1	cells					1687:1691	HEK293T cells	1679:1691	HEK293T cells	1679:1691	Moreover, the N263Q/N354Q variant exhibited altered subcellular distribution within the ER in HEK293T cells, indicating that N-glycosylation of EOGT is required for its ER localization at the cell periphery.
32376684	0	32	theme	EGF	13:15	arg1	transferase					42:52	EGF domain-specific O-GlcNAc transferase	13:52	EGF domain-specific O-GlcNAc transferase (EOGT)	13:59	N-Glycans on EGF domain-specific O-GlcNAc transferase (EOGT) facilitate EOGT maturation and peripheral endoplasmic reticulum localization.
32376684	0	32	theme	EGF	13:15	arg1	EOGT					55:58	EOGT	55:58	EOGT	55:58	N-Glycans on EGF domain-specific O-GlcNAc transferase (EOGT) facilitate EOGT maturation and peripheral endoplasmic reticulum localization.
32376684	8	33	gly	N-glycosylation	1365:1379	arg2	site					1393:1396	a single site	1384:1396	a single site	1384:1396	However, simultaneous substitution of both N-glycosylation sites affected both EOGT maturation and expression levels without an apparent change in enzymatic activity, suggesting that N-glycosylation at a single site is sufficient for EOGT maturation and expression.
32376684	8	33	gly	N-glycosylation	1365:1379	arg1	site					1393:1396	a single site	1384:1396	a single site	1384:1396	However, simultaneous substitution of both N-glycosylation sites affected both EOGT maturation and expression levels without an apparent change in enzymatic activity, suggesting that N-glycosylation at a single site is sufficient for EOGT maturation and expression.
32376684	6	34	theme	Lectin	880:885	arg1	blotting					887:894	Lectin blotting	880:894	Lectin blotting	880:894	Lectin blotting, endoglycosidase H digestion, and MS analysis revealed that both residues are modified with oligomannose N-glycans.
32376684	7	35	from	localization	1097:1108	arg1	cells					1175:1179	HEK293T cells	1167:1179	HEK293T cells	1167:1179	Loss of an individual N-glycan on EOGT did not affect its endoplasmic reticulum (ER) localization, enzyme activity, and ability to O-GlcNAcylate Notch1 in HEK293T cells.
32376684	2	36	theme	N-glycosylation	422:436	arg1	sites					448:452	putative N-glycosylation consensus sites	413:452	putative N-glycosylation consensus sites	413:452	Several EOGT mutations that may affect putative N-glycosylation consensus sites are recorded in the cancer database, but the presence and function of N-glycans in EOGT have not yet been characterized.
32376684	0	37	theme	O-GlcNAc	33:40	arg1	transferase					42:52	EGF domain-specific O-GlcNAc transferase	13:52	EGF domain-specific O-GlcNAc transferase (EOGT)	13:59	N-Glycans on EGF domain-specific O-GlcNAc transferase (EOGT) facilitate EOGT maturation and peripheral endoplasmic reticulum localization.
32376684	0	37	theme	O-GlcNAc	33:40	arg1	EOGT					55:58	EOGT	55:58	EOGT	55:58	N-Glycans on EGF domain-specific O-GlcNAc transferase (EOGT) facilitate EOGT maturation and peripheral endoplasmic reticulum localization.
32376684	1	38	theme	Notch	292:296	arg1	receptors					298:306	Notch receptors	292:306	Notch receptors	292:306	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT) is an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling.
32376684	7	39	theme	O-GlcNAcylate	1143:1155	arg1	Notch1					1157:1162	O-GlcNAcylate Notch1	1143:1162	O-GlcNAcylate Notch1	1143:1162	Loss of an individual N-glycan on EOGT did not affect its endoplasmic reticulum (ER) localization, enzyme activity, and ability to O-GlcNAcylate Notch1 in HEK293T cells.
32376684	1	40	theme	endoplasmic	219:229	arg1	ER					242:243	ER	242:243	ER	242:243	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT) is an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling.
32376684	1	40	theme	endoplasmic	219:229	arg1	reticulum					231:239	endoplasmic reticulum	219:239	an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling	216:371	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT) is an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling.
32376684	9	41	from	decrease	1463:1470	arg1	stoichiometry					1484:1496	O-GlcNAc stoichiometry	1475:1496	O-GlcNAc stoichiometry	1475:1496	Accordingly, a decrease in O-GlcNAc stoichiometry was observed in Notch1 co-expressed with an N263Q/N354Q variant compared with WT EOGT.
32376684	2	42	theme	EOGT	382:385	arg1	mutations					387:395	Several EOGT mutations	374:395	Several EOGT mutations that may affect putative N-glycosylation consensus sites	374:452	Several EOGT mutations that may affect putative N-glycosylation consensus sites are recorded in the cancer database, but the presence and function of N-glycans in EOGT have not yet been characterized.
32376684	6	43	theme	MS	930:931	arg1	analysis					933:940	MS analysis	930:940	MS analysis	930:940	Lectin blotting, endoglycosidase H digestion, and MS analysis revealed that both residues are modified with oligomannose N-glycans.
32376684	10	44	theme	ER	1754:1755	arg1	localization					1757:1768	its ER localization	1750:1768	its ER localization at the cell periphery	1750:1790	Moreover, the N263Q/N354Q variant exhibited altered subcellular distribution within the ER in HEK293T cells, indicating that N-glycosylation of EOGT is required for its ER localization at the cell periphery.
32376684	8	45	from	site	1393:1396	arg1	N-glycosylation					1365:1379	N-glycosylation	1365:1379	N-glycosylation at a single site	1365:1396	However, simultaneous substitution of both N-glycosylation sites affected both EOGT maturation and expression levels without an apparent change in enzymatic activity, suggesting that N-glycosylation at a single site is sufficient for EOGT maturation and expression.
32376684	10	46	theme	N263Q/N354Q	1599:1609	arg1	variant					1611:1617	the N263Q/N354Q variant	1595:1617	the N263Q/N354Q variant	1595:1617	Moreover, the N263Q/N354Q variant exhibited altered subcellular distribution within the ER in HEK293T cells, indicating that N-glycosylation of EOGT is required for its ER localization at the cell periphery.
32376684	6	47	theme	H	913:913	arg1	digestion					915:923	endoglycosidase H digestion	897:923	endoglycosidase H digestion	897:923	Lectin blotting, endoglycosidase H digestion, and MS analysis revealed that both residues are modified with oligomannose N-glycans.
32376684	10	48	theme	cell	1777:1780	arg1	periphery					1782:1790	the cell periphery	1773:1790	the cell periphery	1773:1790	Moreover, the N263Q/N354Q variant exhibited altered subcellular distribution within the ER in HEK293T cells, indicating that N-glycosylation of EOGT is required for its ER localization at the cell periphery.
32376684	4	49	theme	N-glycosylation	689:703	arg1	sequences					715:723	Three predicted N-glycosylation consensus sequences	673:723	Three predicted N-glycosylation consensus sequences on EOGT	673:731	Three predicted N-glycosylation consensus sequences on EOGT are highly conserved among mammalian species.
32376684	1	50	theme	domain-specific	169:183	arg1	transferase					194:204	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase	139:204	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT)	139:211	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT) is an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling.
32376684	1	50	theme	domain-specific	169:183	arg1	protein					255:261	an endoplasmic reticulum (ER)-resident protein	216:261	an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling	216:371	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT) is an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling.
32376684	1	50	theme	domain-specific	169:183	arg1	EOGT					207:210	EOGT	207:210	EOGT	207:210	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT) is an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling.
32376684	8	51	theme	sites	1241:1245	arg1	substitution					1204:1215	simultaneous substitution	1191:1215	simultaneous substitution of both N-glycosylation sites	1191:1245	However, simultaneous substitution of both N-glycosylation sites affected both EOGT maturation and expression levels without an apparent change in enzymatic activity, suggesting that N-glycosylation at a single site is sufficient for EOGT maturation and expression.
32376684	10	52	theme	subcellular	1637:1647	arg1	distribution					1649:1660	altered subcellular distribution	1629:1660	altered subcellular distribution within the ER in HEK293T cells	1629:1691	Moreover, the N263Q/N354Q variant exhibited altered subcellular distribution within the ER in HEK293T cells, indicating that N-glycosylation of EOGT is required for its ER localization at the cell periphery.
32376684	4	53	theme	predicted	679:687	arg1	sequences					715:723	Three predicted N-glycosylation consensus sequences	673:723	Three predicted N-glycosylation consensus sequences on EOGT	673:731	Three predicted N-glycosylation consensus sequences on EOGT are highly conserved among mammalian species.
32376684	7	54	theme	endoplasmic	1070:1080	arg1	ER					1093:1094	ER	1093:1094	ER	1093:1094	Loss of an individual N-glycan on EOGT did not affect its endoplasmic reticulum (ER) localization, enzyme activity, and ability to O-GlcNAcylate Notch1 in HEK293T cells.
32376684	7	54	theme	endoplasmic	1070:1080	arg1	reticulum					1082:1090	its endoplasmic reticulum	1066:1090	its endoplasmic reticulum (ER) localization	1066:1108	Loss of an individual N-glycan on EOGT did not affect its endoplasmic reticulum (ER) localization, enzyme activity, and ability to O-GlcNAcylate Notch1 in HEK293T cells.
32376684	8	55	gly	N-glycosylation	1225:1239	arg2	sites					1241:1245	both N-glycosylation sites	1220:1245	both N-glycosylation sites	1220:1245	However, simultaneous substitution of both N-glycosylation sites affected both EOGT maturation and expression levels without an apparent change in enzymatic activity, suggesting that N-glycosylation at a single site is sufficient for EOGT maturation and expression.
32376684	9	56	theme	O-GlcNAc	1475:1482	arg1	stoichiometry					1484:1496	O-GlcNAc stoichiometry	1475:1496	O-GlcNAc stoichiometry	1475:1496	Accordingly, a decrease in O-GlcNAc stoichiometry was observed in Notch1 co-expressed with an N263Q/N354Q variant compared with WT EOGT.
32376684	2	57	from	presence	499:506	arg1	EOGT					537:540	EOGT	537:540	EOGT	537:540	Several EOGT mutations that may affect putative N-glycosylation consensus sites are recorded in the cancer database, but the presence and function of N-glycans in EOGT have not yet been characterized.
32376684	3	58	gly	N-glycosylation	595:609	arg2	sites					611:615	N-glycosylation sites	595:615	N-glycosylation sites	595:615	Here, we identified N-glycosylation sites in mouse EOGT and elucidated their molecular functions.
32376684	3	59	theme	N-glycosylation	595:609	arg1	sites					611:615	N-glycosylation sites	595:615	N-glycosylation sites	595:615	Here, we identified N-glycosylation sites in mouse EOGT and elucidated their molecular functions.
32376684	7	60	from	Loss	1012:1015	arg1	EOGT					1046:1049	EOGT	1046:1049	EOGT	1046:1049	Loss of an individual N-glycan on EOGT did not affect its endoplasmic reticulum (ER) localization, enzyme activity, and ability to O-GlcNAcylate Notch1 in HEK293T cells.
32376684	2	61	from	function	512:519	arg1	EOGT					537:540	EOGT	537:540	EOGT	537:540	Several EOGT mutations that may affect putative N-glycosylation consensus sites are recorded in the cancer database, but the presence and function of N-glycans in EOGT have not yet been characterized.
32376684	8	62	theme	expression	1281:1290	arg1	levels					1292:1297	expression levels	1281:1297	expression levels	1281:1297	However, simultaneous substitution of both N-glycosylation sites affected both EOGT maturation and expression levels without an apparent change in enzymatic activity, suggesting that N-glycosylation at a single site is sufficient for EOGT maturation and expression.
32376684	7	63	theme	N-glycan	1034:1041	arg1	Loss					1012:1015	Loss	1012:1015	Loss of an individual N-glycan on EOGT	1012:1049	Loss of an individual N-glycan on EOGT did not affect its endoplasmic reticulum (ER) localization, enzyme activity, and ability to O-GlcNAcylate Notch1 in HEK293T cells.
32376684	11	64	theme	O-GlcNAc	1857:1864	arg1	transferase					1866:1876	O-GlcNAc transferase	1857:1876	O-GlcNAc transferase function	1857:1885	These results suggest critical roles of N-glycans in sustaining O-GlcNAc transferase function both by maintaining EOGT levels and by ensuring its proper subcellular localization in the ER.
32376684	1	65	theme	Epidermal	139:147	arg1	EGF					164:166	EGF	164:166	EGF	164:166	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT) is an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling.
32376684	1	65	theme	Epidermal	139:147	arg1	factor					156:161	Epidermal growth factor	139:161	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT)	139:211	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT) is an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling.
32376684	8	66	theme	apparent	1310:1317	arg1	change					1319:1324	an apparent change	1307:1324	an apparent change in enzymatic activity	1307:1346	However, simultaneous substitution of both N-glycosylation sites affected both EOGT maturation and expression levels without an apparent change in enzymatic activity, suggesting that N-glycosylation at a single site is sufficient for EOGT maturation and expression.
32376684	1	67	theme	factor	156:161	arg1	transferase					194:204	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase	139:204	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT)	139:211	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT) is an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling.
32376684	1	67	theme	factor	156:161	arg1	protein					255:261	an endoplasmic reticulum (ER)-resident protein	216:261	an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling	216:371	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT) is an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling.
32376684	1	67	theme	factor	156:161	arg1	EOGT					207:210	EOGT	207:210	EOGT	207:210	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT) is an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling.
32376684	10	68	from	periphery	1782:1790	arg1	localization					1757:1768	its ER localization	1750:1768	its ER localization at the cell periphery	1750:1790	Moreover, the N263Q/N354Q variant exhibited altered subcellular distribution within the ER in HEK293T cells, indicating that N-glycosylation of EOGT is required for its ER localization at the cell periphery.
32376684	4	69	theme	consensus	705:713	arg1	sequences					715:723	Three predicted N-glycosylation consensus sequences	673:723	Three predicted N-glycosylation consensus sequences on EOGT	673:731	Three predicted N-glycosylation consensus sequences on EOGT are highly conserved among mammalian species.
32376684	6	70	mod	modified	974:981	arg3	N-glycans					1001:1009	oligomannose N-glycans	988:1009	oligomannose N-glycans	988:1009	Lectin blotting, endoglycosidase H digestion, and MS analysis revealed that both residues are modified with oligomannose N-glycans.
32376684	6	70	mod	modified	974:981	arg1	residues					961:968	both residues	956:968	both residues	956:968	Lectin blotting, endoglycosidase H digestion, and MS analysis revealed that both residues are modified with oligomannose N-glycans.
32376684	1	71	theme	Delta-like	330:339	arg1	signaling					363:371	Delta-like ligand-mediated Notch signaling	330:371	Delta-like ligand-mediated Notch signaling	330:371	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT) is an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling.
32376684	2	72	attach	presence	499:506	arg2	N-glycans					524:532	N-glycans	524:532	N-glycans	524:532	Several EOGT mutations that may affect putative N-glycosylation consensus sites are recorded in the cancer database, but the presence and function of N-glycans in EOGT have not yet been characterized.
32376684	2	72	attach	presence	499:506	arg1	EOGT					537:540	EOGT	537:540	EOGT	537:540	Several EOGT mutations that may affect putative N-glycosylation consensus sites are recorded in the cancer database, but the presence and function of N-glycans in EOGT have not yet been characterized.
32376684	11	73	theme	critical	1815:1822	arg1	roles					1824:1828	critical roles	1815:1828	critical roles of N-glycans	1815:1841	These results suggest critical roles of N-glycans in sustaining O-GlcNAc transferase function both by maintaining EOGT levels and by ensuring its proper subcellular localization in the ER.
32376684	0	74	theme	peripheral	92:101	arg1	reticulum					115:123	peripheral endoplasmic reticulum	92:123	peripheral endoplasmic reticulum localization	92:136	N-Glycans on EGF domain-specific O-GlcNAc transferase (EOGT) facilitate EOGT maturation and peripheral endoplasmic reticulum localization.
32376684	1	75	theme	Notch	357:361	arg1	signaling					363:371	Delta-like ligand-mediated Notch signaling	330:371	Delta-like ligand-mediated Notch signaling	330:371	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT) is an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling.
32376684	2	76	gly	N-glycosylation	422:436	arg2	sites					448:452	putative N-glycosylation consensus sites	413:452	putative N-glycosylation consensus sites	413:452	Several EOGT mutations that may affect putative N-glycosylation consensus sites are recorded in the cancer database, but the presence and function of N-glycans in EOGT have not yet been characterized.
32376684	0	77	theme	reticulum	115:123	arg1	localization					125:136	peripheral endoplasmic reticulum localization	92:136	peripheral endoplasmic reticulum localization	92:136	N-Glycans on EGF domain-specific O-GlcNAc transferase (EOGT) facilitate EOGT maturation and peripheral endoplasmic reticulum localization.
32376684	7	78	theme	HEK293T	1167:1173	arg1	cells					1175:1179	HEK293T cells	1167:1179	HEK293T cells	1167:1179	Loss of an individual N-glycan on EOGT did not affect its endoplasmic reticulum (ER) localization, enzyme activity, and ability to O-GlcNAcylate Notch1 in HEK293T cells.
32376684	4	79	theme	mammalian	760:768	arg1	species					770:776	mammalian species	760:776	mammalian species	760:776	Three predicted N-glycosylation consensus sequences on EOGT are highly conserved among mammalian species.
32376684	11	80	theme	proper	1939:1944	arg1	localization					1958:1969	its proper subcellular localization	1935:1969	its proper subcellular localization	1935:1969	These results suggest critical roles of N-glycans in sustaining O-GlcNAc transferase function both by maintaining EOGT levels and by ensuring its proper subcellular localization in the ER.
32376684	0	81	theme	domain-specific	17:31	arg1	transferase					42:52	EGF domain-specific O-GlcNAc transferase	13:52	EGF domain-specific O-GlcNAc transferase (EOGT)	13:59	N-Glycans on EGF domain-specific O-GlcNAc transferase (EOGT) facilitate EOGT maturation and peripheral endoplasmic reticulum localization.
32376684	0	81	theme	domain-specific	17:31	arg1	EOGT					55:58	EOGT	55:58	EOGT	55:58	N-Glycans on EGF domain-specific O-GlcNAc transferase (EOGT) facilitate EOGT maturation and peripheral endoplasmic reticulum localization.
32376684	1	82	theme	EGF	277:279	arg1	repeats					281:287	EGF repeats	277:287	EGF repeats of Notch receptors	277:306	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT) is an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling.
32376684	5	83	mod	modified	855:862	arg1	Asn-263					813:819	Asn-263	813:819	Asn-263	813:819	Within these sites, we found that Asn-263 and Asn-354, but not Asn-493, are modified with N-glycans.
32376684	5	83	mod	modified	855:862	arg1	Asn-493					842:848	Asn-493	842:848	Asn-493	842:848	Within these sites, we found that Asn-263 and Asn-354, but not Asn-493, are modified with N-glycans.
32376684	5	83	mod	modified	855:862	arg1	Asn-354					825:831	Asn-354	825:831	Asn-354	825:831	Within these sites, we found that Asn-263 and Asn-354, but not Asn-493, are modified with N-glycans.
32376684	5	83	mod	modified	855:862	arg3	N-glycans					869:877	N-glycans	869:877	N-glycans	869:877	Within these sites, we found that Asn-263 and Asn-354, but not Asn-493, are modified with N-glycans.
32376684	3	84	theme	molecular	652:660	arg1	functions					662:670	their molecular functions	646:670	their molecular functions	646:670	Here, we identified N-glycosylation sites in mouse EOGT and elucidated their molecular functions.
32376684	2	85	theme	consensus	438:446	arg1	sites					448:452	putative N-glycosylation consensus sites	413:452	putative N-glycosylation consensus sites	413:452	Several EOGT mutations that may affect putative N-glycosylation consensus sites are recorded in the cancer database, but the presence and function of N-glycans in EOGT have not yet been characterized.
32376684	2	86	theme	putative	413:420	arg1	sites					448:452	putative N-glycosylation consensus sites	413:452	putative N-glycosylation consensus sites	413:452	Several EOGT mutations that may affect putative N-glycosylation consensus sites are recorded in the cancer database, but the presence and function of N-glycans in EOGT have not yet been characterized.
32376684	1	87	theme	receptors	298:306	arg1	repeats					281:287	EGF repeats	277:287	EGF repeats of Notch receptors	277:306	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT) is an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling.
32376684	2	88	dep	presence	499:506	arg1	the					495:497	the	495:497	the	495:497	Several EOGT mutations that may affect putative N-glycosylation consensus sites are recorded in the cancer database, but the presence and function of N-glycans in EOGT have not yet been characterized.
32376684	2	89	theme	cancer	474:479	arg1	database					481:488	the cancer database	470:488	the cancer database	470:488	Several EOGT mutations that may affect putative N-glycosylation consensus sites are recorded in the cancer database, but the presence and function of N-glycans in EOGT have not yet been characterized.
32376684	7	90	from	activity	1118:1125	arg1	cells					1175:1179	HEK293T cells	1167:1179	HEK293T cells	1167:1179	Loss of an individual N-glycan on EOGT did not affect its endoplasmic reticulum (ER) localization, enzyme activity, and ability to O-GlcNAcylate Notch1 in HEK293T cells.
32376684	8	91	theme	EOGT	1416:1419	arg1	maturation					1421:1430	EOGT maturation	1416:1430	EOGT maturation	1416:1430	However, simultaneous substitution of both N-glycosylation sites affected both EOGT maturation and expression levels without an apparent change in enzymatic activity, suggesting that N-glycosylation at a single site is sufficient for EOGT maturation and expression.
32376684	11	92	theme	EOGT	1907:1910	arg1	levels					1912:1917	EOGT levels	1907:1917	EOGT levels	1907:1917	These results suggest critical roles of N-glycans in sustaining O-GlcNAc transferase function both by maintaining EOGT levels and by ensuring its proper subcellular localization in the ER.
32376684	10	93	theme	EOGT	1729:1732	arg1	N-glycosylation					1710:1724	N-glycosylation	1710:1724	N-glycosylation of EOGT	1710:1732	Moreover, the N263Q/N354Q variant exhibited altered subcellular distribution within the ER in HEK293T cells, indicating that N-glycosylation of EOGT is required for its ER localization at the cell periphery.
32376684	1	94	theme	reticulum	231:239	arg1	transferase					194:204	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase	139:204	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT)	139:211	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT) is an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling.
32376684	1	94	theme	reticulum	231:239	arg1	protein					255:261	an endoplasmic reticulum (ER)-resident protein	216:261	an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling	216:371	Epidermal growth factor (EGF) domain-specific O-GlcNAc transferase (EOGT) is an endoplasmic reticulum (ER)-resident protein that modifies EGF repeats of Notch receptors and thereby regulates Delta-like ligand-mediated Notch signaling.
32376684	2	95	theme	Several	374:380	arg1	mutations					387:395	Several EOGT mutations	374:395	Several EOGT mutations that may affect putative N-glycosylation consensus sites	374:452	Several EOGT mutations that may affect putative N-glycosylation consensus sites are recorded in the cancer database, but the presence and function of N-glycans in EOGT have not yet been characterized.
33780884	0	0	theme	Dansyl	125:130	arg1	acids					138:142	Dansyl amino acids	125:142	Dansyl amino acids	125:142	Facile preparation of ethanediamine-β-cyclodextrin modified capillary column for electrochromatographic enantioseparation of Dansyl amino acids.
33780884	2	1	theme	surface	533:539	arg1	wall					541:544	the surface wall	529:544	the surface wall of the capillary	529:561	A facile interfacial polymerization was used to anchor ethanediamine-β-cyclodextrin (EDA-β-CD) polymerized with trimesoyl chloride (TMC) and to form the chiral stationary phase (CSP) composite onto the surface wall of the capillary.
33780884	1	2	theme	multifunctional	186:200	arg1	phase					238:242	a fascinating multifunctional cyclodextrin (CD) chiral stationary phase	172:242	a fascinating multifunctional cyclodextrin (CD) chiral stationary phase	172:242	Herein, the fabrication of a fascinating multifunctional cyclodextrin (CD) chiral stationary phase and its chiral separation performance in capillary electrochromatography are proposed.
33780884	1	3	theme	capillary	285:293	arg1	electrochromatography					295:315	capillary electrochromatography	285:315	capillary electrochromatography	285:315	Herein, the fabrication of a fascinating multifunctional cyclodextrin (CD) chiral stationary phase and its chiral separation performance in capillary electrochromatography are proposed.
33780884	8	4	theme	application	1533:1543	arg1	view					1545:1548	a broader application view	1523:1548	a broader application view	1523:1548	This innovative chiral stationary phase shows a broader application view and scope in chiral recognition domain.
33780884	4	5	theme	interaction	911:921	arg1	effects					941:947	hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects	848:947	hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects which contribute to prominent chiral recognition for Dansyl-DL-amino acids in CEC modes	848:1035	This novel CSP offers multi-typical interactions including hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects which contribute to prominent chiral recognition for Dansyl-DL-amino acids in CEC modes.
33780884	1	6	theme	cyclodextrin	202:213	arg1	phase					238:242	a fascinating multifunctional cyclodextrin (CD) chiral stationary phase	172:242	a fascinating multifunctional cyclodextrin (CD) chiral stationary phase	172:242	Herein, the fabrication of a fascinating multifunctional cyclodextrin (CD) chiral stationary phase and its chiral separation performance in capillary electrochromatography are proposed.
33780884	4	7	from	acids	1018:1022	arg1	modes					1031:1035	CEC modes	1027:1035	CEC modes	1027:1035	This novel CSP offers multi-typical interactions including hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects which contribute to prominent chiral recognition for Dansyl-DL-amino acids in CEC modes.
33780884	0	8	theme	acids	138:142	arg1	enantioseparation					104:120	electrochromatographic enantioseparation	81:120	electrochromatographic enantioseparation of Dansyl amino acids	81:142	Facile preparation of ethanediamine-β-cyclodextrin modified capillary column for electrochromatographic enantioseparation of Dansyl amino acids.
33780884	5	9	theme	modified	1051:1058	arg1	column					1060:1065	The EDA-β-CD modified column	1038:1065	The EDA-β-CD modified column	1038:1065	The EDA-β-CD modified column showed eminent enantioseparation performance towards five Dansyl-DL-amino acids (the DL-forms of valine, threonine, leucine, phenylalanine, serine).
33780884	2	10	theme	interfacial	340:350	arg1	polymerization					352:365	A facile interfacial polymerization	331:365	A facile interfacial polymerization	331:365	A facile interfacial polymerization was used to anchor ethanediamine-β-cyclodextrin (EDA-β-CD) polymerized with trimesoyl chloride (TMC) and to form the chiral stationary phase (CSP) composite onto the surface wall of the capillary.
33780884	5	11	theme	valine	1164:1169	arg1	DL-forms					1152:1159	the DL-forms	1148:1159	the DL-forms of valine, threonine, leucine, phenylalanine, serine	1148:1212	The EDA-β-CD modified column showed eminent enantioseparation performance towards five Dansyl-DL-amino acids (the DL-forms of valine, threonine, leucine, phenylalanine, serine).
33780884	3	12	theme	columns	591:597	arg1	characters					568:577	The characters	564:577	The characters of prepared columns	564:597	The characters of prepared columns were confirmed by Fourier transform infrared spectroscopy (FT-IR), X-ray Photoelectron Spectrometer (XPS), scanning electron microscopy (SEM) and energy dispersive X-ray spectrometry (EDS).
33780884	0	13	theme	amino	132:136	arg1	acids					138:142	Dansyl amino acids	125:142	Dansyl amino acids	125:142	Facile preparation of ethanediamine-β-cyclodextrin modified capillary column for electrochromatographic enantioseparation of Dansyl amino acids.
33780884	3	14	theme	dispersive	752:761	arg1	EDS					783:785	EDS	783:785	EDS	783:785	The characters of prepared columns were confirmed by Fourier transform infrared spectroscopy (FT-IR), X-ray Photoelectron Spectrometer (XPS), scanning electron microscopy (SEM) and energy dispersive X-ray spectrometry (EDS).
33780884	3	14	theme	dispersive	752:761	arg1	spectrometry					769:780	energy dispersive X-ray spectrometry	745:780	energy dispersive X-ray spectrometry (EDS)	745:786	The characters of prepared columns were confirmed by Fourier transform infrared spectroscopy (FT-IR), X-ray Photoelectron Spectrometer (XPS), scanning electron microscopy (SEM) and energy dispersive X-ray spectrometry (EDS).
33780884	2	15	theme	facile	333:338	arg1	polymerization					352:365	A facile interfacial polymerization	331:365	A facile interfacial polymerization	331:365	A facile interfacial polymerization was used to anchor ethanediamine-β-cyclodextrin (EDA-β-CD) polymerized with trimesoyl chloride (TMC) and to form the chiral stationary phase (CSP) composite onto the surface wall of the capillary.
33780884	2	16	theme	polymerized	426:436	arg1	EDA-β-CD					416:423	EDA-β-CD	416:423	EDA-β-CD	416:423	A facile interfacial polymerization was used to anchor ethanediamine-β-cyclodextrin (EDA-β-CD) polymerized with trimesoyl chloride (TMC) and to form the chiral stationary phase (CSP) composite onto the surface wall of the capillary.
33780884	2	16	theme	polymerized	426:436	arg1	ethanediamine-β-cyclodextrin					386:413	anchor ethanediamine-β-cyclodextrin	379:413	anchor ethanediamine-β-cyclodextrin (EDA-β-CD) polymerized with trimesoyl chloride (TMC)	379:466	A facile interfacial polymerization was used to anchor ethanediamine-β-cyclodextrin (EDA-β-CD) polymerized with trimesoyl chloride (TMC) and to form the chiral stationary phase (CSP) composite onto the surface wall of the capillary.
33780884	7	17	theme	column-to-columns	1403:1419	arg1	deviations					1308:1317	The relative standard deviations	1286:1317	The relative standard deviations of the enantiomer retention times for intra-day (n = 5), inter-day (n = 3) runs and column-to-columns (n = 3)	1286:1427	The relative standard deviations of the enantiomer retention times for intra-day (n = 5), inter-day (n = 3) runs and column-to-columns (n = 3) are below 0.54%, 1.35% and 4.89%, individually.
33780884	7	18	theme	times	1347:1351	arg1	deviations					1308:1317	The relative standard deviations	1286:1317	The relative standard deviations of the enantiomer retention times for intra-day (n = 5), inter-day (n = 3) runs and column-to-columns (n = 3)	1286:1427	The relative standard deviations of the enantiomer retention times for intra-day (n = 5), inter-day (n = 3) runs and column-to-columns (n = 3) are below 0.54%, 1.35% and 4.89%, individually.
33780884	2	19	theme	capillary	553:561	arg1	wall					541:544	the surface wall	529:544	the surface wall of the capillary	529:561	A facile interfacial polymerization was used to anchor ethanediamine-β-cyclodextrin (EDA-β-CD) polymerized with trimesoyl chloride (TMC) and to form the chiral stationary phase (CSP) composite onto the surface wall of the capillary.
33780884	3	20	theme	X-ray	763:767	arg1	EDS					783:785	EDS	783:785	EDS	783:785	The characters of prepared columns were confirmed by Fourier transform infrared spectroscopy (FT-IR), X-ray Photoelectron Spectrometer (XPS), scanning electron microscopy (SEM) and energy dispersive X-ray spectrometry (EDS).
33780884	3	20	theme	X-ray	763:767	arg1	spectrometry					769:780	energy dispersive X-ray spectrometry	745:780	energy dispersive X-ray spectrometry (EDS)	745:786	The characters of prepared columns were confirmed by Fourier transform infrared spectroscopy (FT-IR), X-ray Photoelectron Spectrometer (XPS), scanning electron microscopy (SEM) and energy dispersive X-ray spectrometry (EDS).
33780884	4	21	theme	hydrophobic	881:891	arg1	effects					941:947	hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects	848:947	hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects which contribute to prominent chiral recognition for Dansyl-DL-amino acids in CEC modes	848:1035	This novel CSP offers multi-typical interactions including hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects which contribute to prominent chiral recognition for Dansyl-DL-amino acids in CEC modes.
33780884	6	22	theme	prepared	1229:1236	arg1	columns					1238:1244	the prepared columns	1225:1244	the prepared columns	1225:1244	Besides, the prepared columns were perfectly reproducible and stable.
33780884	6	22	theme	prepared	1229:1236	arg1	reproducible					1261:1272	reproducible	1261:1272	reproducible	1261:1272	Besides, the prepared columns were perfectly reproducible and stable.
33780884	3	23	dep	transform	625:633	arg1	infrared					635:642	infrared	635:642	transform infrared spectroscopy (FT-IR), X-ray Photoelectron Spectrometer (XPS), scanning electron microscopy (SEM) and energy dispersive X-ray spectrometry (EDS)	625:786	The characters of prepared columns were confirmed by Fourier transform infrared spectroscopy (FT-IR), X-ray Photoelectron Spectrometer (XPS), scanning electron microscopy (SEM) and energy dispersive X-ray spectrometry (EDS).
33780884	8	24	from	view	1545:1548	arg1	domain					1582:1587	chiral recognition domain	1563:1587	chiral recognition domain	1563:1587	This innovative chiral stationary phase shows a broader application view and scope in chiral recognition domain.
33780884	5	25	dep	acids	1141:1145	arg1	DL-forms					1152:1159	the DL-forms	1148:1159	the DL-forms of valine, threonine, leucine, phenylalanine, serine	1148:1212	The EDA-β-CD modified column showed eminent enantioseparation performance towards five Dansyl-DL-amino acids (the DL-forms of valine, threonine, leucine, phenylalanine, serine).
33780884	1	26	from	fabrication	157:167	arg1	electrochromatography					295:315	capillary electrochromatography	285:315	capillary electrochromatography	285:315	Herein, the fabrication of a fascinating multifunctional cyclodextrin (CD) chiral stationary phase and its chiral separation performance in capillary electrochromatography are proposed.
33780884	2	27	theme	phase	502:506	arg1	composite					514:522	the chiral stationary phase (CSP) composite	480:522	the chiral stationary phase (CSP) composite onto the surface wall of the capillary	480:561	A facile interfacial polymerization was used to anchor ethanediamine-β-cyclodextrin (EDA-β-CD) polymerized with trimesoyl chloride (TMC) and to form the chiral stationary phase (CSP) composite onto the surface wall of the capillary.
33780884	8	28	theme	innovative	1482:1491	arg1	phase					1511:1515	This innovative chiral stationary phase	1477:1515	This innovative chiral stationary phase	1477:1515	This innovative chiral stationary phase shows a broader application view and scope in chiral recognition domain.
33780884	1	29	theme	CD	216:217	arg1	phase					238:242	a fascinating multifunctional cyclodextrin (CD) chiral stationary phase	172:242	a fascinating multifunctional cyclodextrin (CD) chiral stationary phase	172:242	Herein, the fabrication of a fascinating multifunctional cyclodextrin (CD) chiral stationary phase and its chiral separation performance in capillary electrochromatography are proposed.
33780884	5	30	theme	enantioseparation	1082:1098	arg1	performance					1100:1110	eminent enantioseparation performance	1074:1110	eminent enantioseparation performance towards five Dansyl-DL-amino acids (the DL-forms of valine, threonine, leucine, phenylalanine, serine)	1074:1213	The EDA-β-CD modified column showed eminent enantioseparation performance towards five Dansyl-DL-amino acids (the DL-forms of valine, threonine, leucine, phenylalanine, serine).
33780884	3	31	theme	energy	745:750	arg1	EDS					783:785	EDS	783:785	EDS	783:785	The characters of prepared columns were confirmed by Fourier transform infrared spectroscopy (FT-IR), X-ray Photoelectron Spectrometer (XPS), scanning electron microscopy (SEM) and energy dispersive X-ray spectrometry (EDS).
33780884	3	31	theme	energy	745:750	arg1	spectrometry					769:780	energy dispersive X-ray spectrometry	745:780	energy dispersive X-ray spectrometry (EDS)	745:786	The characters of prepared columns were confirmed by Fourier transform infrared spectroscopy (FT-IR), X-ray Photoelectron Spectrometer (XPS), scanning electron microscopy (SEM) and energy dispersive X-ray spectrometry (EDS).
33780884	4	32	theme	electrostatic	897:909	arg1	effects					941:947	hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects	848:947	hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects which contribute to prominent chiral recognition for Dansyl-DL-amino acids in CEC modes	848:1035	This novel CSP offers multi-typical interactions including hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects which contribute to prominent chiral recognition for Dansyl-DL-amino acids in CEC modes.
33780884	7	33	theme	inter-day	1376:1384	arg1	runs					1394:1397	inter-day (n = 3) runs	1376:1397	inter-day (n = 3) runs	1376:1397	The relative standard deviations of the enantiomer retention times for intra-day (n = 5), inter-day (n = 3) runs and column-to-columns (n = 3) are below 0.54%, 1.35% and 4.89%, individually.
33780884	0	34	theme	Facile	0:5	arg1	preparation					7:17	Facile preparation	0:17	Facile preparation of ethanediamine-β-cyclodextrin	0:49	Facile preparation of ethanediamine-β-cyclodextrin modified capillary column for electrochromatographic enantioseparation of Dansyl amino acids.
33780884	8	35	theme	recognition	1570:1580	arg1	domain					1582:1587	chiral recognition domain	1563:1587	chiral recognition domain	1563:1587	This innovative chiral stationary phase shows a broader application view and scope in chiral recognition domain.
33780884	3	36	theme	scanning	706:713	arg1	SEM					736:738	SEM	736:738	SEM	736:738	The characters of prepared columns were confirmed by Fourier transform infrared spectroscopy (FT-IR), X-ray Photoelectron Spectrometer (XPS), scanning electron microscopy (SEM) and energy dispersive X-ray spectrometry (EDS).
33780884	3	36	theme	scanning	706:713	arg1	microscopy					724:733	scanning electron microscopy	706:733	scanning electron microscopy (SEM)	706:739	The characters of prepared columns were confirmed by Fourier transform infrared spectroscopy (FT-IR), X-ray Photoelectron Spectrometer (XPS), scanning electron microscopy (SEM) and energy dispersive X-ray spectrometry (EDS).
33780884	1	37	theme	chiral	220:225	arg1	phase					238:242	a fascinating multifunctional cyclodextrin (CD) chiral stationary phase	172:242	a fascinating multifunctional cyclodextrin (CD) chiral stationary phase	172:242	Herein, the fabrication of a fascinating multifunctional cyclodextrin (CD) chiral stationary phase and its chiral separation performance in capillary electrochromatography are proposed.
33780884	0	38	theme	ethanediamine-β-cyclodextrin	22:49	arg1	preparation					7:17	Facile preparation	0:17	Facile preparation of ethanediamine-β-cyclodextrin	0:49	Facile preparation of ethanediamine-β-cyclodextrin modified capillary column for electrochromatographic enantioseparation of Dansyl amino acids.
33780884	8	39	theme	chiral	1563:1568	arg1	domain					1582:1587	chiral recognition domain	1563:1587	chiral recognition domain	1563:1587	This innovative chiral stationary phase shows a broader application view and scope in chiral recognition domain.
33780884	4	40	theme	prominent	969:977	arg1	recognition					986:996	prominent chiral recognition	969:996	prominent chiral recognition for Dansyl-DL-amino acids in CEC modes	969:1035	This novel CSP offers multi-typical interactions including hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects which contribute to prominent chiral recognition for Dansyl-DL-amino acids in CEC modes.
33780884	5	41	theme	eminent	1074:1080	arg1	performance					1100:1110	eminent enantioseparation performance	1074:1110	eminent enantioseparation performance towards five Dansyl-DL-amino acids (the DL-forms of valine, threonine, leucine, phenylalanine, serine)	1074:1213	The EDA-β-CD modified column showed eminent enantioseparation performance towards five Dansyl-DL-amino acids (the DL-forms of valine, threonine, leucine, phenylalanine, serine).
33780884	1	42	theme	stationary	227:236	arg1	phase					238:242	a fascinating multifunctional cyclodextrin (CD) chiral stationary phase	172:242	a fascinating multifunctional cyclodextrin (CD) chiral stationary phase	172:242	Herein, the fabrication of a fascinating multifunctional cyclodextrin (CD) chiral stationary phase and its chiral separation performance in capillary electrochromatography are proposed.
33780884	2	43	theme	chiral	484:489	arg1	phase					502:506	the chiral stationary phase	480:506	the chiral stationary phase (CSP) composite onto the surface wall of the capillary	480:561	A facile interfacial polymerization was used to anchor ethanediamine-β-cyclodextrin (EDA-β-CD) polymerized with trimesoyl chloride (TMC) and to form the chiral stationary phase (CSP) composite onto the surface wall of the capillary.
33780884	2	43	theme	chiral	484:489	arg1	CSP					509:511	CSP	509:511	CSP	509:511	A facile interfacial polymerization was used to anchor ethanediamine-β-cyclodextrin (EDA-β-CD) polymerized with trimesoyl chloride (TMC) and to form the chiral stationary phase (CSP) composite onto the surface wall of the capillary.
33780884	8	44	from	scope	1554:1558	arg1	domain					1582:1587	chiral recognition domain	1563:1587	chiral recognition domain	1563:1587	This innovative chiral stationary phase shows a broader application view and scope in chiral recognition domain.
33780884	3	45	dep	Fourier	617:623	arg1	transform					625:633	transform	625:633	transform infrared spectroscopy (FT-IR), X-ray Photoelectron Spectrometer (XPS), scanning electron microscopy (SEM) and energy dispersive X-ray spectrometry (EDS)	625:786	The characters of prepared columns were confirmed by Fourier transform infrared spectroscopy (FT-IR), X-ray Photoelectron Spectrometer (XPS), scanning electron microscopy (SEM) and energy dispersive X-ray spectrometry (EDS).
33780884	1	46	theme	phase	238:242	arg1	fabrication					157:167	the fabrication	153:167	the fabrication of a fascinating multifunctional cyclodextrin (CD) chiral stationary phase	153:242	Herein, the fabrication of a fascinating multifunctional cyclodextrin (CD) chiral stationary phase and its chiral separation performance in capillary electrochromatography are proposed.
33780884	1	46	theme	phase	238:242	arg1	performance					270:280	its chiral separation performance	248:280	its chiral separation performance in capillary electrochromatography	248:315	Herein, the fabrication of a fascinating multifunctional cyclodextrin (CD) chiral stationary phase and its chiral separation performance in capillary electrochromatography are proposed.
33780884	0	47	theme	capillary	60:68	arg1	column					70:75	capillary column	60:75	capillary column for electrochromatographic enantioseparation of Dansyl amino acids	60:142	Facile preparation of ethanediamine-β-cyclodextrin modified capillary column for electrochromatographic enantioseparation of Dansyl amino acids.
33780884	4	48	theme	bonding	857:863	arg1	effects					941:947	hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects	848:947	hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects which contribute to prominent chiral recognition for Dansyl-DL-amino acids in CEC modes	848:1035	This novel CSP offers multi-typical interactions including hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects which contribute to prominent chiral recognition for Dansyl-DL-amino acids in CEC modes.
33780884	7	49	theme	n = 3	1387:1391	arg1	runs					1394:1397	inter-day (n = 3) runs	1376:1397	inter-day (n = 3) runs	1376:1397	The relative standard deviations of the enantiomer retention times for intra-day (n = 5), inter-day (n = 3) runs and column-to-columns (n = 3) are below 0.54%, 1.35% and 4.89%, individually.
33780884	4	50	theme	chiral	979:984	arg1	recognition					986:996	prominent chiral recognition	969:996	prominent chiral recognition for Dansyl-DL-amino acids in CEC modes	969:1035	This novel CSP offers multi-typical interactions including hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects which contribute to prominent chiral recognition for Dansyl-DL-amino acids in CEC modes.
33780884	3	51	theme	Photoelectron	672:684	arg1	XPS					700:702	XPS	700:702	XPS	700:702	The characters of prepared columns were confirmed by Fourier transform infrared spectroscopy (FT-IR), X-ray Photoelectron Spectrometer (XPS), scanning electron microscopy (SEM) and energy dispersive X-ray spectrometry (EDS).
33780884	3	51	theme	Photoelectron	672:684	arg1	Spectrometer					686:697	X-ray Photoelectron Spectrometer	666:697	X-ray Photoelectron Spectrometer (XPS)	666:703	The characters of prepared columns were confirmed by Fourier transform infrared spectroscopy (FT-IR), X-ray Photoelectron Spectrometer (XPS), scanning electron microscopy (SEM) and energy dispersive X-ray spectrometry (EDS).
33780884	2	52	theme	stationary	491:500	arg1	phase					502:506	the chiral stationary phase	480:506	the chiral stationary phase (CSP) composite onto the surface wall of the capillary	480:561	A facile interfacial polymerization was used to anchor ethanediamine-β-cyclodextrin (EDA-β-CD) polymerized with trimesoyl chloride (TMC) and to form the chiral stationary phase (CSP) composite onto the surface wall of the capillary.
33780884	2	52	theme	stationary	491:500	arg1	CSP					509:511	CSP	509:511	CSP	509:511	A facile interfacial polymerization was used to anchor ethanediamine-β-cyclodextrin (EDA-β-CD) polymerized with trimesoyl chloride (TMC) and to form the chiral stationary phase (CSP) composite onto the surface wall of the capillary.
33780884	8	53	theme	chiral	1493:1498	arg1	phase					1511:1515	This innovative chiral stationary phase	1477:1515	This innovative chiral stationary phase	1477:1515	This innovative chiral stationary phase shows a broader application view and scope in chiral recognition domain.
33780884	4	54	theme	Dansyl-DL-amino	1002:1016	arg1	acids					1018:1022	Dansyl-DL-amino acids	1002:1022	Dansyl-DL-amino acids in CEC modes	1002:1035	This novel CSP offers multi-typical interactions including hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects which contribute to prominent chiral recognition for Dansyl-DL-amino acids in CEC modes.
33780884	1	55	from	performance	270:280	arg1	electrochromatography					295:315	capillary electrochromatography	285:315	capillary electrochromatography	285:315	Herein, the fabrication of a fascinating multifunctional cyclodextrin (CD) chiral stationary phase and its chiral separation performance in capillary electrochromatography are proposed.
33780884	4	56	theme	CEC	1027:1029	arg1	modes					1031:1035	CEC modes	1027:1035	CEC modes	1027:1035	This novel CSP offers multi-typical interactions including hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects which contribute to prominent chiral recognition for Dansyl-DL-amino acids in CEC modes.
33780884	4	57	theme	π-interaction	866:878	arg1	effects					941:947	hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects	848:947	hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects which contribute to prominent chiral recognition for Dansyl-DL-amino acids in CEC modes	848:1035	This novel CSP offers multi-typical interactions including hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects which contribute to prominent chiral recognition for Dansyl-DL-amino acids in CEC modes.
33780884	8	58	theme	broader	1525:1531	arg1	application					1533:1543	a broader application	1523:1543	a broader application view	1523:1548	This innovative chiral stationary phase shows a broader application view and scope in chiral recognition domain.
33780884	4	59	theme	steric	934:939	arg1	effects					941:947	hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects	848:947	hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects which contribute to prominent chiral recognition for Dansyl-DL-amino acids in CEC modes	848:1035	This novel CSP offers multi-typical interactions including hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects which contribute to prominent chiral recognition for Dansyl-DL-amino acids in CEC modes.
33780884	2	60	with	polymerized	426:436	arg1	TMC					463:465	TMC	463:465	TMC	463:465	A facile interfacial polymerization was used to anchor ethanediamine-β-cyclodextrin (EDA-β-CD) polymerized with trimesoyl chloride (TMC) and to form the chiral stationary phase (CSP) composite onto the surface wall of the capillary.
33780884	2	60	with	polymerized	426:436	arg1	chloride					453:460	trimesoyl chloride	443:460	trimesoyl chloride (TMC)	443:466	A facile interfacial polymerization was used to anchor ethanediamine-β-cyclodextrin (EDA-β-CD) polymerized with trimesoyl chloride (TMC) and to form the chiral stationary phase (CSP) composite onto the surface wall of the capillary.
33780884	5	61	theme	Dansyl-DL-amino	1125:1139	arg1	acids					1141:1145	five Dansyl-DL-amino acids	1120:1145	five Dansyl-DL-amino acids (the DL-forms of valine, threonine, leucine, phenylalanine, serine)	1120:1213	The EDA-β-CD modified column showed eminent enantioseparation performance towards five Dansyl-DL-amino acids (the DL-forms of valine, threonine, leucine, phenylalanine, serine).
33780884	7	62	theme	enantiomer	1326:1335	arg1	times					1347:1351	the enantiomer retention times	1322:1351	the enantiomer retention times for intra-day (n = 5)	1322:1373	The relative standard deviations of the enantiomer retention times for intra-day (n = 5), inter-day (n = 3) runs and column-to-columns (n = 3) are below 0.54%, 1.35% and 4.89%, individually.
33780884	3	63	theme	electron	715:722	arg1	SEM					736:738	SEM	736:738	SEM	736:738	The characters of prepared columns were confirmed by Fourier transform infrared spectroscopy (FT-IR), X-ray Photoelectron Spectrometer (XPS), scanning electron microscopy (SEM) and energy dispersive X-ray spectrometry (EDS).
33780884	3	63	theme	electron	715:722	arg1	microscopy					724:733	scanning electron microscopy	706:733	scanning electron microscopy (SEM)	706:739	The characters of prepared columns were confirmed by Fourier transform infrared spectroscopy (FT-IR), X-ray Photoelectron Spectrometer (XPS), scanning electron microscopy (SEM) and energy dispersive X-ray spectrometry (EDS).
33780884	4	64	theme	multi-typical	811:823	arg1	interactions					825:836	multi-typical interactions	811:836	multi-typical interactions including hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects which contribute to prominent chiral recognition for Dansyl-DL-amino acids in CEC modes	811:1035	This novel CSP offers multi-typical interactions including hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects which contribute to prominent chiral recognition for Dansyl-DL-amino acids in CEC modes.
33780884	4	64	theme	multi-typical	811:823	arg1	effects					941:947	hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects	848:947	hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects which contribute to prominent chiral recognition for Dansyl-DL-amino acids in CEC modes	848:1035	This novel CSP offers multi-typical interactions including hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects which contribute to prominent chiral recognition for Dansyl-DL-amino acids in CEC modes.
33780884	4	65	theme	hydrogen	848:855	arg1	effects					941:947	hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects	848:947	hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects which contribute to prominent chiral recognition for Dansyl-DL-amino acids in CEC modes	848:1035	This novel CSP offers multi-typical interactions including hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects which contribute to prominent chiral recognition for Dansyl-DL-amino acids in CEC modes.
33780884	7	66	theme	retention	1337:1345	arg1	times					1347:1351	the enantiomer retention times	1322:1351	the enantiomer retention times for intra-day (n = 5)	1322:1373	The relative standard deviations of the enantiomer retention times for intra-day (n = 5), inter-day (n = 3) runs and column-to-columns (n = 3) are below 0.54%, 1.35% and 4.89%, individually.
33780884	1	67	theme	chiral	252:257	arg1	performance					270:280	its chiral separation performance	248:280	its chiral separation performance in capillary electrochromatography	248:315	Herein, the fabrication of a fascinating multifunctional cyclodextrin (CD) chiral stationary phase and its chiral separation performance in capillary electrochromatography are proposed.
33780884	4	68	theme	novel	794:798	arg1	CSP					800:802	This novel CSP	789:802	This novel CSP	789:802	This novel CSP offers multi-typical interactions including hydrogen bonding, π-interaction, hydrophobic and electrostatic interaction as well as steric effects which contribute to prominent chiral recognition for Dansyl-DL-amino acids in CEC modes.
33780884	3	69	theme	X-ray	666:670	arg1	XPS					700:702	XPS	700:702	XPS	700:702	The characters of prepared columns were confirmed by Fourier transform infrared spectroscopy (FT-IR), X-ray Photoelectron Spectrometer (XPS), scanning electron microscopy (SEM) and energy dispersive X-ray spectrometry (EDS).
33780884	3	69	theme	X-ray	666:670	arg1	Spectrometer					686:697	X-ray Photoelectron Spectrometer	666:697	X-ray Photoelectron Spectrometer (XPS)	666:703	The characters of prepared columns were confirmed by Fourier transform infrared spectroscopy (FT-IR), X-ray Photoelectron Spectrometer (XPS), scanning electron microscopy (SEM) and energy dispersive X-ray spectrometry (EDS).
33780884	2	70	theme	anchor	379:384	arg1	EDA-β-CD					416:423	EDA-β-CD	416:423	EDA-β-CD	416:423	A facile interfacial polymerization was used to anchor ethanediamine-β-cyclodextrin (EDA-β-CD) polymerized with trimesoyl chloride (TMC) and to form the chiral stationary phase (CSP) composite onto the surface wall of the capillary.
33780884	2	70	theme	anchor	379:384	arg1	ethanediamine-β-cyclodextrin					386:413	anchor ethanediamine-β-cyclodextrin	379:413	anchor ethanediamine-β-cyclodextrin (EDA-β-CD) polymerized with trimesoyl chloride (TMC)	379:466	A facile interfacial polymerization was used to anchor ethanediamine-β-cyclodextrin (EDA-β-CD) polymerized with trimesoyl chloride (TMC) and to form the chiral stationary phase (CSP) composite onto the surface wall of the capillary.
33780884	2	71	used	used	371:374	arg2	polymerization					352:365	A facile interfacial polymerization	331:365	A facile interfacial polymerization	331:365	A facile interfacial polymerization was used to anchor ethanediamine-β-cyclodextrin (EDA-β-CD) polymerized with trimesoyl chloride (TMC) and to form the chiral stationary phase (CSP) composite onto the surface wall of the capillary.
33780884	1	72	theme	separation	259:268	arg1	performance					270:280	its chiral separation performance	248:280	its chiral separation performance in capillary electrochromatography	248:315	Herein, the fabrication of a fascinating multifunctional cyclodextrin (CD) chiral stationary phase and its chiral separation performance in capillary electrochromatography are proposed.
33780884	7	73	theme	relative	1290:1297	arg1	deviations					1308:1317	The relative standard deviations	1286:1317	The relative standard deviations of the enantiomer retention times for intra-day (n = 5), inter-day (n = 3) runs and column-to-columns (n = 3)	1286:1427	The relative standard deviations of the enantiomer retention times for intra-day (n = 5), inter-day (n = 3) runs and column-to-columns (n = 3) are below 0.54%, 1.35% and 4.89%, individually.
33780884	8	74	theme	stationary	1500:1509	arg1	phase					1511:1515	This innovative chiral stationary phase	1477:1515	This innovative chiral stationary phase	1477:1515	This innovative chiral stationary phase shows a broader application view and scope in chiral recognition domain.
33780884	7	75	theme	runs	1394:1397	arg1	deviations					1308:1317	The relative standard deviations	1286:1317	The relative standard deviations of the enantiomer retention times for intra-day (n = 5), inter-day (n = 3) runs and column-to-columns (n = 3)	1286:1427	The relative standard deviations of the enantiomer retention times for intra-day (n = 5), inter-day (n = 3) runs and column-to-columns (n = 3) are below 0.54%, 1.35% and 4.89%, individually.
33780884	2	76	theme	trimesoyl	443:451	arg1	TMC					463:465	TMC	463:465	TMC	463:465	A facile interfacial polymerization was used to anchor ethanediamine-β-cyclodextrin (EDA-β-CD) polymerized with trimesoyl chloride (TMC) and to form the chiral stationary phase (CSP) composite onto the surface wall of the capillary.
33780884	2	76	theme	trimesoyl	443:451	arg1	chloride					453:460	trimesoyl chloride	443:460	trimesoyl chloride (TMC)	443:466	A facile interfacial polymerization was used to anchor ethanediamine-β-cyclodextrin (EDA-β-CD) polymerized with trimesoyl chloride (TMC) and to form the chiral stationary phase (CSP) composite onto the surface wall of the capillary.
33780884	7	77	theme	standard	1299:1306	arg1	deviations					1308:1317	The relative standard deviations	1286:1317	The relative standard deviations of the enantiomer retention times for intra-day (n = 5), inter-day (n = 3) runs and column-to-columns (n = 3)	1286:1427	The relative standard deviations of the enantiomer retention times for intra-day (n = 5), inter-day (n = 3) runs and column-to-columns (n = 3) are below 0.54%, 1.35% and 4.89%, individually.
33780884	0	78	theme	electrochromatographic	81:102	arg1	enantioseparation					104:120	electrochromatographic enantioseparation	81:120	electrochromatographic enantioseparation of Dansyl amino acids	81:142	Facile preparation of ethanediamine-β-cyclodextrin modified capillary column for electrochromatographic enantioseparation of Dansyl amino acids.
33780884	3	79	theme	prepared	582:589	arg1	columns					591:597	prepared columns	582:597	prepared columns	582:597	The characters of prepared columns were confirmed by Fourier transform infrared spectroscopy (FT-IR), X-ray Photoelectron Spectrometer (XPS), scanning electron microscopy (SEM) and energy dispersive X-ray spectrometry (EDS).
33780884	1	80	theme	fascinating	174:184	arg1	phase					238:242	a fascinating multifunctional cyclodextrin (CD) chiral stationary phase	172:242	a fascinating multifunctional cyclodextrin (CD) chiral stationary phase	172:242	Herein, the fabrication of a fascinating multifunctional cyclodextrin (CD) chiral stationary phase and its chiral separation performance in capillary electrochromatography are proposed.
32355592	0	0	theme	lycopersicum	82:93	arg1	fruit					96:100	tomato (Solanum lycopersicum) fruit	66:100	tomato (Solanum lycopersicum) fruit	66:100	Suppression of N-glycan processing enzymes by deoxynojirimycin in tomato (Solanum lycopersicum) fruit.
32355592	8	1	from	5-fold	1480:1485	arg1	methylesterases					1449:1463	aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases	1382:1463	methylesterases	1449:1463	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	8	1	from	5-fold	1480:1485	arg1	genes					1334:1338	important ripening-related and softening process-associated genes	1274:1338	important ripening-related and softening process-associated genes	1274:1338	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	7	2	theme	cell	1091:1094	arg1	softening					1101:1109	cell wall softening	1091:1109	cell wall softening	1091:1109	Enzyme activity of the N-glycan processing enzymes involved in cell wall softening, α-mannosidase and β-d-N-acetylhexosaminidase revealed a significant reduction in their activity by 2 and 3.5-fold, respectively.
32355592	4	3	theme	treatment	640:648	arg1	influence					619:627	the influence	615:627	the influence of the DNJ treatment of the fruit	615:661	Analysis of the influence of the DNJ treatment of the fruit using quadratic multiple regression models considering the factors colour, texture, and free sugars revealed significant responses.
32355592	9	4	theme	present	1525:1531	arg1	results					1533:1539	The present results	1521:1539	The present results	1521:1539	The present results showed that the treatment of mature green tomato fruit with DNJ at a concentration of 0.30 mM can delay the ripening of the tomato fruit by inhibiting cell wall and N-glycan processing enzymes.
32355592	0	5	theme	Solanum	74:80	arg1	fruit					96:100	tomato (Solanum lycopersicum) fruit	66:100	tomato (Solanum lycopersicum) fruit	66:100	Suppression of N-glycan processing enzymes by deoxynojirimycin in tomato (Solanum lycopersicum) fruit.
32355592	7	6	theme	wall	1096:1099	arg1	softening					1101:1109	cell wall softening	1091:1109	cell wall softening	1091:1109	Enzyme activity of the N-glycan processing enzymes involved in cell wall softening, α-mannosidase and β-d-N-acetylhexosaminidase revealed a significant reduction in their activity by 2 and 3.5-fold, respectively.
32355592	1	7	theme	small	153:157	arg1	1-deoxynojirimycin					179:196	1-deoxynojirimycin	179:196	1-deoxynojirimycin (DNJ)	179:202	The present study investigated the potential of a small molecule inhibitor, 1-deoxynojirimycin (DNJ), to extend the shelf life of tomatoes.
32355592	1	7	theme	small	153:157	arg1	inhibitor					168:176	a small molecule inhibitor	151:176	a small molecule inhibitor	151:176	The present study investigated the potential of a small molecule inhibitor, 1-deoxynojirimycin (DNJ), to extend the shelf life of tomatoes.
32355592	9	8	from	concentration	1610:1622	arg1	DNJ					1601:1603	DNJ	1601:1603	DNJ at a concentration of 0.30 mM	1601:1633	The present results showed that the treatment of mature green tomato fruit with DNJ at a concentration of 0.30 mM can delay the ripening of the tomato fruit by inhibiting cell wall and N-glycan processing enzymes.
32355592	8	9	theme	polygalacturonase	1420:1436	arg1	methylesterases					1449:1463	aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases	1382:1463	methylesterases	1449:1463	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	8	9	theme	polygalacturonase	1420:1436	arg1	genes					1334:1338	important ripening-related and softening process-associated genes	1274:1338	important ripening-related and softening process-associated genes	1274:1338	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	9	10	theme	tomato	1665:1670	arg1	fruit					1672:1676	the tomato fruit	1661:1676	the tomato fruit	1661:1676	The present results showed that the treatment of mature green tomato fruit with DNJ at a concentration of 0.30 mM can delay the ripening of the tomato fruit by inhibiting cell wall and N-glycan processing enzymes.
32355592	5	11	theme	stage	845:849	arg1	optimal					883:889	optimal	883:889	optimal	883:889	A DNJ concentration of 0.30 mM and fruit-ripening stage of mature green was found to be optimal for the treatment.
32355592	5	11	theme	stage	845:849	arg1	concentration					801:813	A DNJ concentration	795:813	A DNJ concentration of 0.30 mM and fruit-ripening stage of mature green	795:865	A DNJ concentration of 0.30 mM and fruit-ripening stage of mature green was found to be optimal for the treatment.
32355592	3	12	theme	fruit	522:526	arg1	0.30 mM					476:482	0.30 mM	476:482	0.30 mM	476:482	The concentration of DNJ used for the analysis was 0.15 mM, and 0.30 mM and the ripening stages of the tomato fruit analysed were immature green, mature green, breaker, ripen and over-ripen.
32355592	3	12	theme	fruit	522:526	arg1	stages					501:506	the ripening stages	488:506	the ripening stages of the tomato fruit	488:526	The concentration of DNJ used for the analysis was 0.15 mM, and 0.30 mM and the ripening stages of the tomato fruit analysed were immature green, mature green, breaker, ripen and over-ripen.
32355592	3	12	theme	fruit	522:526	arg1	green					551:555	green	551:555	green	551:555	The concentration of DNJ used for the analysis was 0.15 mM, and 0.30 mM and the ripening stages of the tomato fruit analysed were immature green, mature green, breaker, ripen and over-ripen.
32355592	1	13	theme	tomatoes	233:240	arg1	life					225:228	the shelf life	215:228	the shelf life of tomatoes	215:240	The present study investigated the potential of a small molecule inhibitor, 1-deoxynojirimycin (DNJ), to extend the shelf life of tomatoes.
32355592	4	14	theme	multiple	679:686	arg1	models					699:704	quadratic multiple regression models	669:704	quadratic multiple regression models considering the factors colour, texture, and free sugars	669:761	Analysis of the influence of the DNJ treatment of the fruit using quadratic multiple regression models considering the factors colour, texture, and free sugars revealed significant responses.
32355592	9	15	theme	fruit	1672:1676	arg1	ripening					1649:1656	the ripening	1645:1656	the ripening of the tomato fruit	1645:1676	The present results showed that the treatment of mature green tomato fruit with DNJ at a concentration of 0.30 mM can delay the ripening of the tomato fruit by inhibiting cell wall and N-glycan processing enzymes.
32355592	8	16	theme	carboxylic	1359:1368	arg1	synthase-4					1370:1379	aminocyclopropane carboxylic synthase-4	1341:1379	aminocyclopropane carboxylic synthase-4	1341:1379	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	8	16	theme	carboxylic	1359:1368	arg1	genes					1334:1338	important ripening-related and softening process-associated genes	1274:1338	important ripening-related and softening process-associated genes	1274:1338	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	8	17	from	4	1468:1468	arg1	methylesterases					1449:1463	aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases	1382:1463	methylesterases	1449:1463	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	8	17	from	4	1468:1468	arg1	genes					1334:1338	important ripening-related and softening process-associated genes	1274:1338	important ripening-related and softening process-associated genes	1274:1338	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	4	18	theme	fruit	657:661	arg1	influence					619:627	the influence	615:627	the influence of the DNJ treatment of the fruit	615:661	Analysis of the influence of the DNJ treatment of the fruit using quadratic multiple regression models considering the factors colour, texture, and free sugars revealed significant responses.
32355592	4	19	theme	DNJ	636:638	arg1	treatment					640:648	the DNJ treatment	632:648	the DNJ treatment	632:648	Analysis of the influence of the DNJ treatment of the fruit using quadratic multiple regression models considering the factors colour, texture, and free sugars revealed significant responses.
32355592	0	20	from	deoxynojirimycin	46:61	arg1	fruit					96:100	tomato (Solanum lycopersicum) fruit	66:100	tomato (Solanum lycopersicum) fruit	66:100	Suppression of N-glycan processing enzymes by deoxynojirimycin in tomato (Solanum lycopersicum) fruit.
32355592	7	21	theme	processing	1060:1069	arg1	enzymes					1071:1077	the N-glycan processing enzymes	1047:1077	the N-glycan processing enzymes involved in cell wall softening, α-mannosidase and β-d-N-acetylhexosaminidase	1047:1155	Enzyme activity of the N-glycan processing enzymes involved in cell wall softening, α-mannosidase and β-d-N-acetylhexosaminidase revealed a significant reduction in their activity by 2 and 3.5-fold, respectively.
32355592	6	22	theme	sugar	1011:1015	arg1	formation					1017:1025	sugar formation	1011:1025	sugar formation	1011:1025	DNJ-treatment maintained fruit firmness throughout ripening with a significant reduction in reducing sugar formation.
32355592	2	23	theme	optimum	247:253	arg1	concentration					255:267	The optimum concentration	243:267	The optimum concentration of DNJ and the proper ripening stage for treatment	243:318	The optimum concentration of DNJ and the proper ripening stage for treatment were standardized using response surface methodology, following a central composite design.
32355592	5	24	theme	0.30 mM	818:824	arg1	optimal					883:889	optimal	883:889	optimal	883:889	A DNJ concentration of 0.30 mM and fruit-ripening stage of mature green was found to be optimal for the treatment.
32355592	5	24	theme	0.30 mM	818:824	arg1	concentration					801:813	A DNJ concentration	795:813	A DNJ concentration of 0.30 mM and fruit-ripening stage of mature green	795:865	A DNJ concentration of 0.30 mM and fruit-ripening stage of mature green was found to be optimal for the treatment.
32355592	1	25	theme	molecule	159:166	arg1	1-deoxynojirimycin					179:196	1-deoxynojirimycin	179:196	1-deoxynojirimycin (DNJ)	179:202	The present study investigated the potential of a small molecule inhibitor, 1-deoxynojirimycin (DNJ), to extend the shelf life of tomatoes.
32355592	1	25	theme	molecule	159:166	arg1	inhibitor					168:176	a small molecule inhibitor	151:176	a small molecule inhibitor	151:176	The present study investigated the potential of a small molecule inhibitor, 1-deoxynojirimycin (DNJ), to extend the shelf life of tomatoes.
32355592	6	26	theme	fruit	935:939	arg1	firmness					941:948	fruit firmness	935:948	fruit firmness	935:948	DNJ-treatment maintained fruit firmness throughout ripening with a significant reduction in reducing sugar formation.
32355592	5	27	theme	mature	854:859	arg1	green					861:865	mature green	854:865	mature green	854:865	A DNJ concentration of 0.30 mM and fruit-ripening stage of mature green was found to be optimal for the treatment.
32355592	7	28	theme	enzymes	1071:1077	arg1	activity					1035:1042	Enzyme activity	1028:1042	Enzyme activity of the N-glycan processing enzymes involved in cell wall softening, α-mannosidase and β-d-N-acetylhexosaminidase	1028:1155	Enzyme activity of the N-glycan processing enzymes involved in cell wall softening, α-mannosidase and β-d-N-acetylhexosaminidase revealed a significant reduction in their activity by 2 and 3.5-fold, respectively.
32355592	8	29	theme	aminocyclopropane	1382:1398	arg1	methylesterases					1449:1463	aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases	1382:1463	methylesterases	1449:1463	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	8	29	theme	aminocyclopropane	1382:1398	arg1	genes					1334:1338	important ripening-related and softening process-associated genes	1274:1338	important ripening-related and softening process-associated genes	1274:1338	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	1	30	theme	inhibitor	168:176	arg1	potential					138:146	the potential	134:146	the potential of a small molecule inhibitor, 1-deoxynojirimycin (DNJ), to extend the shelf life of tomatoes	134:240	The present study investigated the potential of a small molecule inhibitor, 1-deoxynojirimycin (DNJ), to extend the shelf life of tomatoes.
32355592	9	31	theme	cell	1692:1695	arg1	wall					1697:1700	cell wall	1692:1700	cell wall	1692:1700	The present results showed that the treatment of mature green tomato fruit with DNJ at a concentration of 0.30 mM can delay the ripening of the tomato fruit by inhibiting cell wall and N-glycan processing enzymes.
32355592	2	32	theme	proper	284:289	arg1	stage					300:304	the proper ripening stage	280:304	the proper ripening stage for treatment	280:318	The optimum concentration of DNJ and the proper ripening stage for treatment were standardized using response surface methodology, following a central composite design.
32355592	4	33	theme	influence	619:627	arg1	Analysis					603:610	Analysis	603:610	Analysis of the influence of the DNJ treatment of the fruit using quadratic multiple regression models considering the factors colour, texture, and free sugars	603:761	Analysis of the influence of the DNJ treatment of the fruit using quadratic multiple regression models considering the factors colour, texture, and free sugars revealed significant responses.
32355592	7	34	theme	significant	1168:1178	arg1	reduction					1180:1188	a significant reduction	1166:1188	a significant reduction in their activity by 2 and 3.5-fold, respectively	1166:1238	Enzyme activity of the N-glycan processing enzymes involved in cell wall softening, α-mannosidase and β-d-N-acetylhexosaminidase revealed a significant reduction in their activity by 2 and 3.5-fold, respectively.
32355592	9	35	theme	fruit	1590:1594	arg1	treatment					1557:1565	the treatment	1553:1565	the treatment of mature green tomato fruit with DNJ at a concentration of 0.30 mM	1553:1633	The present results showed that the treatment of mature green tomato fruit with DNJ at a concentration of 0.30 mM can delay the ripening of the tomato fruit by inhibiting cell wall and N-glycan processing enzymes.
32355592	7	36	theme	N-glycan	1051:1058	arg1	enzymes					1071:1077	the N-glycan processing enzymes	1047:1077	the N-glycan processing enzymes involved in cell wall softening, α-mannosidase and β-d-N-acetylhexosaminidase	1047:1155	Enzyme activity of the N-glycan processing enzymes involved in cell wall softening, α-mannosidase and β-d-N-acetylhexosaminidase revealed a significant reduction in their activity by 2 and 3.5-fold, respectively.
32355592	4	37	theme	quadratic	669:677	arg1	models					699:704	quadratic multiple regression models	669:704	quadratic multiple regression models considering the factors colour, texture, and free sugars	669:761	Analysis of the influence of the DNJ treatment of the fruit using quadratic multiple regression models considering the factors colour, texture, and free sugars revealed significant responses.
32355592	8	38	theme	carboxylic	1400:1409	arg1	methylesterases					1449:1463	aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases	1382:1463	methylesterases	1449:1463	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	8	38	theme	carboxylic	1400:1409	arg1	genes					1334:1338	important ripening-related and softening process-associated genes	1274:1338	important ripening-related and softening process-associated genes	1274:1338	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	2	39	theme	surface	353:359	arg1	methodology					361:371	response surface methodology	344:371	response surface methodology	344:371	The optimum concentration of DNJ and the proper ripening stage for treatment were standardized using response surface methodology, following a central composite design.
32355592	0	40	theme	processing	24:33	arg1	enzymes					35:41	N-glycan processing enzymes	15:41	N-glycan processing enzymes	15:41	Suppression of N-glycan processing enzymes by deoxynojirimycin in tomato (Solanum lycopersicum) fruit.
32355592	8	41	theme	process-associated	1315:1332	arg1	synthase-4					1370:1379	aminocyclopropane carboxylic synthase-4	1341:1379	aminocyclopropane carboxylic synthase-4	1341:1379	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	8	41	theme	process-associated	1315:1332	arg1	methylesterases					1449:1463	aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases	1382:1463	methylesterases	1449:1463	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	8	41	theme	process-associated	1315:1332	arg1	genes					1334:1338	important ripening-related and softening process-associated genes	1274:1338	important ripening-related and softening process-associated genes	1274:1338	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	3	42	theme	tomato	515:520	arg1	fruit					522:526	the tomato fruit	511:526	the tomato fruit	511:526	The concentration of DNJ used for the analysis was 0.15 mM, and 0.30 mM and the ripening stages of the tomato fruit analysed were immature green, mature green, breaker, ripen and over-ripen.
32355592	8	43	theme	expression	1260:1269	arg1	Down-regulation					1241:1255	Down-regulation	1241:1255	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively,	1241:1500	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	0	44	theme	N-glycan	15:22	arg1	enzymes					35:41	N-glycan processing enzymes	15:41	N-glycan processing enzymes	15:41	Suppression of N-glycan processing enzymes by deoxynojirimycin in tomato (Solanum lycopersicum) fruit.
32355592	3	45	theme	ripening	492:499	arg1	stages					501:506	the ripening stages	488:506	the ripening stages of the tomato fruit	488:526	The concentration of DNJ used for the analysis was 0.15 mM, and 0.30 mM and the ripening stages of the tomato fruit analysed were immature green, mature green, breaker, ripen and over-ripen.
32355592	3	45	theme	ripening	492:499	arg1	green					551:555	green	551:555	green	551:555	The concentration of DNJ used for the analysis was 0.15 mM, and 0.30 mM and the ripening stages of the tomato fruit analysed were immature green, mature green, breaker, ripen and over-ripen.
32355592	7	46	from	reduction	1180:1188	arg1	activity					1199:1206	their activity	1193:1206	their activity	1193:1206	Enzyme activity of the N-glycan processing enzymes involved in cell wall softening, α-mannosidase and β-d-N-acetylhexosaminidase revealed a significant reduction in their activity by 2 and 3.5-fold, respectively.
32355592	2	47	theme	DNJ	272:274	arg1	concentration					255:267	The optimum concentration	243:267	The optimum concentration of DNJ and the proper ripening stage for treatment	243:318	The optimum concentration of DNJ and the proper ripening stage for treatment were standardized using response surface methodology, following a central composite design.
32355592	8	48	from	5	1471:1471	arg1	methylesterases					1449:1463	aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases	1382:1463	methylesterases	1449:1463	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	8	48	from	5	1471:1471	arg1	genes					1334:1338	important ripening-related and softening process-associated genes	1274:1338	important ripening-related and softening process-associated genes	1274:1338	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	2	49	theme	composite	394:402	arg1	design					404:409	a central composite design	384:409	a central composite design	384:409	The optimum concentration of DNJ and the proper ripening stage for treatment were standardized using response surface methodology, following a central composite design.
32355592	8	50	theme	aminocyclopropane	1341:1357	arg1	synthase-4					1370:1379	aminocyclopropane carboxylic synthase-4	1341:1379	aminocyclopropane carboxylic synthase-4	1341:1379	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	8	50	theme	aminocyclopropane	1341:1357	arg1	genes					1334:1338	important ripening-related and softening process-associated genes	1274:1338	important ripening-related and softening process-associated genes	1274:1338	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	9	51	theme	processing	1715:1724	arg1	enzymes					1726:1732	N-glycan processing enzymes	1706:1732	N-glycan processing enzymes	1706:1732	The present results showed that the treatment of mature green tomato fruit with DNJ at a concentration of 0.30 mM can delay the ripening of the tomato fruit by inhibiting cell wall and N-glycan processing enzymes.
32355592	3	52	theme	DNJ	433:435	arg1	concentration					416:428	The concentration	412:428	The concentration of DNJ used for the analysis	412:457	The concentration of DNJ used for the analysis was 0.15 mM, and 0.30 mM and the ripening stages of the tomato fruit analysed were immature green, mature green, breaker, ripen and over-ripen.
32355592	3	52	theme	DNJ	433:435	arg1	0.15 mM					463:469	0.15 mM	463:469	0.15 mM	463:469	The concentration of DNJ used for the analysis was 0.15 mM, and 0.30 mM and the ripening stages of the tomato fruit analysed were immature green, mature green, breaker, ripen and over-ripen.
32355592	8	53	from	6	1474:1474	arg1	methylesterases					1449:1463	aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases	1382:1463	methylesterases	1449:1463	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	8	53	from	6	1474:1474	arg1	genes					1334:1338	important ripening-related and softening process-associated genes	1274:1338	important ripening-related and softening process-associated genes	1274:1338	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	2	54	theme	central	386:392	arg1	design					404:409	a central composite design	384:409	a central composite design	384:409	The optimum concentration of DNJ and the proper ripening stage for treatment were standardized using response surface methodology, following a central composite design.
32355592	0	55	theme	enzymes	35:41	arg1	Suppression					0:10	Suppression	0:10	Suppression of N-glycan processing enzymes by deoxynojirimycin in tomato (Solanum lycopersicum) fruit.	0:101	Suppression of N-glycan processing enzymes by deoxynojirimycin in tomato (Solanum lycopersicum) fruit.
32355592	7	56	theme	Enzyme	1028:1033	arg1	activity					1035:1042	Enzyme activity	1028:1042	Enzyme activity of the N-glycan processing enzymes involved in cell wall softening, α-mannosidase and β-d-N-acetylhexosaminidase	1028:1155	Enzyme activity of the N-glycan processing enzymes involved in cell wall softening, α-mannosidase and β-d-N-acetylhexosaminidase revealed a significant reduction in their activity by 2 and 3.5-fold, respectively.
32355592	8	57	theme	genes	1334:1338	arg1	expression					1260:1269	expression	1260:1269	expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively,	1260:1500	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	4	58	theme	free	751:754	arg1	sugars					756:761	free sugars	751:761	free sugars	751:761	Analysis of the influence of the DNJ treatment of the fruit using quadratic multiple regression models considering the factors colour, texture, and free sugars revealed significant responses.
32355592	5	59	theme	DNJ	797:799	arg1	optimal					883:889	optimal	883:889	optimal	883:889	A DNJ concentration of 0.30 mM and fruit-ripening stage of mature green was found to be optimal for the treatment.
32355592	5	59	theme	DNJ	797:799	arg1	concentration					801:813	A DNJ concentration	795:813	A DNJ concentration of 0.30 mM and fruit-ripening stage of mature green	795:865	A DNJ concentration of 0.30 mM and fruit-ripening stage of mature green was found to be optimal for the treatment.
32355592	5	60	theme	fruit-ripening	830:843	arg1	stage					845:849	fruit-ripening stage	830:849	fruit-ripening stage	830:849	A DNJ concentration of 0.30 mM and fruit-ripening stage of mature green was found to be optimal for the treatment.
32355592	4	61	theme	significant	772:782	arg1	responses					784:792	significant responses	772:792	significant responses	772:792	Analysis of the influence of the DNJ treatment of the fruit using quadratic multiple regression models considering the factors colour, texture, and free sugars revealed significant responses.
32355592	8	62	theme	oxidase	1411:1417	arg1	methylesterases					1449:1463	aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases	1382:1463	methylesterases	1449:1463	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	8	62	theme	oxidase	1411:1417	arg1	genes					1334:1338	important ripening-related and softening process-associated genes	1274:1338	important ripening-related and softening process-associated genes	1274:1338	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	1	63	theme	present	107:113	arg1	study					115:119	The present study	103:119	The present study	103:119	The present study investigated the potential of a small molecule inhibitor, 1-deoxynojirimycin (DNJ), to extend the shelf life of tomatoes.
32355592	9	64	theme	0.30 mM	1627:1633	arg1	concentration					1610:1622	a concentration	1608:1622	a concentration of 0.30 mM	1608:1633	The present results showed that the treatment of mature green tomato fruit with DNJ at a concentration of 0.30 mM can delay the ripening of the tomato fruit by inhibiting cell wall and N-glycan processing enzymes.
32355592	9	65	with	treatment	1557:1565	arg1	DNJ					1601:1603	DNJ	1601:1603	DNJ at a concentration of 0.30 mM	1601:1633	The present results showed that the treatment of mature green tomato fruit with DNJ at a concentration of 0.30 mM can delay the ripening of the tomato fruit by inhibiting cell wall and N-glycan processing enzymes.
32355592	8	66	theme	ripening-related	1284:1299	arg1	synthase-4					1370:1379	aminocyclopropane carboxylic synthase-4	1341:1379	aminocyclopropane carboxylic synthase-4	1341:1379	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	8	66	theme	ripening-related	1284:1299	arg1	methylesterases					1449:1463	aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases	1382:1463	methylesterases	1449:1463	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	8	66	theme	ripening-related	1284:1299	arg1	genes					1334:1338	important ripening-related and softening process-associated genes	1274:1338	important ripening-related and softening process-associated genes	1274:1338	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	8	67	theme	important	1274:1282	arg1	synthase-4					1370:1379	aminocyclopropane carboxylic synthase-4	1341:1379	aminocyclopropane carboxylic synthase-4	1341:1379	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	8	67	theme	important	1274:1282	arg1	methylesterases					1449:1463	aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases	1382:1463	methylesterases	1449:1463	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	8	67	theme	important	1274:1282	arg1	genes					1334:1338	important ripening-related and softening process-associated genes	1274:1338	important ripening-related and softening process-associated genes	1274:1338	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	8	68	theme	pectin	1442:1447	arg1	methylesterases					1449:1463	aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases	1382:1463	methylesterases	1449:1463	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	8	68	theme	pectin	1442:1447	arg1	genes					1334:1338	important ripening-related and softening process-associated genes	1274:1338	important ripening-related and softening process-associated genes	1274:1338	Down-regulation of expression of important ripening-related and softening process-associated genes, aminocyclopropane carboxylic synthase-4, aminocyclopropane carboxylic oxidase, polygalacturonase and pectin methylesterases at 4, 5, 6 and 5-fold, respectively, was also observed.
32355592	2	69	theme	stage	300:304	arg1	concentration					255:267	The optimum concentration	243:267	The optimum concentration of DNJ and the proper ripening stage for treatment	243:318	The optimum concentration of DNJ and the proper ripening stage for treatment were standardized using response surface methodology, following a central composite design.
32355592	9	70	theme	tomato	1583:1588	arg1	fruit					1590:1594	mature green tomato fruit	1570:1594	mature green tomato fruit	1570:1594	The present results showed that the treatment of mature green tomato fruit with DNJ at a concentration of 0.30 mM can delay the ripening of the tomato fruit by inhibiting cell wall and N-glycan processing enzymes.
32355592	5	71	theme	green	861:865	arg1	0.30 mM					818:824	0.30 mM	818:824	0.30 mM	818:824	A DNJ concentration of 0.30 mM and fruit-ripening stage of mature green was found to be optimal for the treatment.
32355592	5	71	theme	green	861:865	arg1	stage					845:849	fruit-ripening stage	830:849	fruit-ripening stage	830:849	A DNJ concentration of 0.30 mM and fruit-ripening stage of mature green was found to be optimal for the treatment.
32355592	4	72	theme	regression	688:697	arg1	models					699:704	quadratic multiple regression models	669:704	quadratic multiple regression models considering the factors colour, texture, and free sugars	669:761	Analysis of the influence of the DNJ treatment of the fruit using quadratic multiple regression models considering the factors colour, texture, and free sugars revealed significant responses.
32355592	2	73	theme	ripening	291:298	arg1	stage					300:304	the proper ripening stage	280:304	the proper ripening stage for treatment	280:318	The optimum concentration of DNJ and the proper ripening stage for treatment were standardized using response surface methodology, following a central composite design.
32355592	6	74	theme	significant	977:987	arg1	reduction					989:997	a significant reduction	975:997	a significant reduction in reducing sugar formation	975:1025	DNJ-treatment maintained fruit firmness throughout ripening with a significant reduction in reducing sugar formation.
32355592	2	75	theme	response	344:351	arg1	methodology					361:371	response surface methodology	344:371	response surface methodology	344:371	The optimum concentration of DNJ and the proper ripening stage for treatment were standardized using response surface methodology, following a central composite design.
32355592	9	76	theme	mature	1570:1575	arg1	fruit					1590:1594	mature green tomato fruit	1570:1594	mature green tomato fruit	1570:1594	The present results showed that the treatment of mature green tomato fruit with DNJ at a concentration of 0.30 mM can delay the ripening of the tomato fruit by inhibiting cell wall and N-glycan processing enzymes.
32355592	0	77	theme	tomato	66:71	arg1	fruit					96:100	tomato (Solanum lycopersicum) fruit	66:100	tomato (Solanum lycopersicum) fruit	66:100	Suppression of N-glycan processing enzymes by deoxynojirimycin in tomato (Solanum lycopersicum) fruit.
32355592	9	78	theme	green	1577:1581	arg1	fruit					1590:1594	mature green tomato fruit	1570:1594	mature green tomato fruit	1570:1594	The present results showed that the treatment of mature green tomato fruit with DNJ at a concentration of 0.30 mM can delay the ripening of the tomato fruit by inhibiting cell wall and N-glycan processing enzymes.
32355592	4	79	dep	factors	722:728	arg1	sugars					756:761	free sugars	751:761	free sugars	751:761	Analysis of the influence of the DNJ treatment of the fruit using quadratic multiple regression models considering the factors colour, texture, and free sugars revealed significant responses.
32355592	4	79	dep	factors	722:728	arg1	factors					722:728	the factors colour, texture, and free sugars	718:761	the factors colour, texture, and free sugars	718:761	Analysis of the influence of the DNJ treatment of the fruit using quadratic multiple regression models considering the factors colour, texture, and free sugars revealed significant responses.
32355592	4	79	dep	factors	722:728	arg1	texture					738:744	texture	738:744	texture	738:744	Analysis of the influence of the DNJ treatment of the fruit using quadratic multiple regression models considering the factors colour, texture, and free sugars revealed significant responses.
32355592	4	79	dep	factors	722:728	arg1	colour					730:735	colour	730:735	colour	730:735	Analysis of the influence of the DNJ treatment of the fruit using quadratic multiple regression models considering the factors colour, texture, and free sugars revealed significant responses.
32355592	9	80	theme	N-glycan	1706:1713	arg1	enzymes					1726:1732	N-glycan processing enzymes	1706:1732	N-glycan processing enzymes	1706:1732	The present results showed that the treatment of mature green tomato fruit with DNJ at a concentration of 0.30 mM can delay the ripening of the tomato fruit by inhibiting cell wall and N-glycan processing enzymes.
32355592	1	81	theme	shelf	219:223	arg1	life					225:228	the shelf life	215:228	the shelf life of tomatoes	215:240	The present study investigated the potential of a small molecule inhibitor, 1-deoxynojirimycin (DNJ), to extend the shelf life of tomatoes.
32307668	6	0	theme	muscle	1115:1120	arg1	αSMA					1129:1132	αSMA	1129:1132	αSMA	1129:1132	In addition, the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells upregulated while cultured in the presence of curcumin, but this increase was significantly improved while cells cultured on the nanofibers/curcumin.
32307668	6	0	theme	muscle	1115:1120	arg1	actin					1122:1126	alpha-smooth muscle actin	1102:1126	alpha-smooth muscle actin (αSMA)	1102:1133	In addition, the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells upregulated while cultured in the presence of curcumin, but this increase was significantly improved while cells cultured on the nanofibers/curcumin.
32307668	2	1	from	engineering	310:320	arg1	decades					338:344	the last two decades	325:344	the last two decades	325:344	Tissue engineering in the last two decades has given great hope for the treatment of these disorders.
32307668	4	2	theme	supportive	590:599	arg1	functions					601:609	The scaffold supportive functions	577:609	The scaffold supportive functions from smooth muscle cell differentiation	577:649	The scaffold supportive functions from smooth muscle cell differentiation were studied when human-induced pluripotent stem cells were cultured on the scaffolds under differentiation medium.
32307668	0	3	theme	chitosan/collagen/polyvinyl-alcohol	83:117	arg1	nanofibers					119:128	curcumin-incorporated chitosan/collagen/polyvinyl-alcohol nanofibers	61:128	curcumin-incorporated chitosan/collagen/polyvinyl-alcohol nanofibers	61:128	Efficient smooth muscle cell differentiation of iPS cells on curcumin-incorporated chitosan/collagen/polyvinyl-alcohol nanofibers.
32307668	8	4	theme	smooth	1555:1560	arg1	differentiation					1574:1588	smooth muscle cell differentiation	1555:1588	smooth muscle cell differentiation of the induced pluripotent stem cells promoted by curcumin and this promotion	1555:1666	It can be concluded that smooth muscle cell differentiation of the induced pluripotent stem cells promoted by curcumin and this promotion was synergistically improved while curcumin incorporated in the nanofibers.
32307668	3	5	theme	polyvinyl-alcohol	497:513	arg1	blend					523:527	polyvinyl-alcohol polymer blend	497:527	polyvinyl-alcohol polymer blend	497:527	In this study, a composite nanofibrous scaffold was fabricated from chitosan, collagen, and polyvinyl-alcohol polymer blend while curcumin incorporated in scaffold fibers.
32307668	4	6	theme	scaffold	581:588	arg1	functions					601:609	The scaffold supportive functions	577:609	The scaffold supportive functions from smooth muscle cell differentiation	577:649	The scaffold supportive functions from smooth muscle cell differentiation were studied when human-induced pluripotent stem cells were cultured on the scaffolds under differentiation medium.
32307668	5	7	from	curcumin	852:859	arg1	fibers					877:882	the scaffold fibers	864:882	the scaffold fibers	864:882	Biocompatibility of the fabricated scaffold increased significantly by incorporating curcumin in the scaffold fibers, where protein adsorption, cell attachment, and viability were increased in the nanofiber/curcumin group compared with the other groups.
32307668	2	8	theme	Tissue	303:308	arg1	engineering					310:320	Tissue engineering	303:320	Tissue engineering in the last two decades	303:344	Tissue engineering in the last two decades has given great hope for the treatment of these disorders.
32307668	4	9	theme	stem	695:698	arg1	cells					700:704	human-induced pluripotent stem cells	669:704	human-induced pluripotent stem cells	669:704	The scaffold supportive functions from smooth muscle cell differentiation were studied when human-induced pluripotent stem cells were cultured on the scaffolds under differentiation medium.
32307668	6	10	dep	actin	1122:1126	arg1	cells					1206:1210	the stem cells	1197:1210	the stem cells	1197:1210	In addition, the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells upregulated while cultured in the presence of curcumin, but this increase was significantly improved while cells cultured on the nanofibers/curcumin.
32307668	8	11	theme	cell	1569:1572	arg1	differentiation					1574:1588	smooth muscle cell differentiation	1555:1588	smooth muscle cell differentiation of the induced pluripotent stem cells promoted by curcumin and this promotion	1555:1666	It can be concluded that smooth muscle cell differentiation of the induced pluripotent stem cells promoted by curcumin and this promotion was synergistically improved while curcumin incorporated in the nanofibers.
32307668	2	12	dep	great	356:360	arg1	hope					362:365	hope	362:365	hope	362:365	Tissue engineering in the last two decades has given great hope for the treatment of these disorders.
32307668	6	13	theme	expression	1038:1047	arg1	level					1049:1053	the expression level	1034:1053	the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells	1034:1210	In addition, the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells upregulated while cultured in the presence of curcumin, but this increase was significantly improved while cells cultured on the nanofibers/curcumin.
32307668	5	14	theme	nanofiber/curcumin	964:981	arg1	group					983:987	the nanofiber/curcumin group	960:987	the nanofiber/curcumin group	960:987	Biocompatibility of the fabricated scaffold increased significantly by incorporating curcumin in the scaffold fibers, where protein adsorption, cell attachment, and viability were increased in the nanofiber/curcumin group compared with the other groups.
32307668	5	15	theme	other	1007:1011	arg1	groups					1013:1018	the other groups	1003:1018	the other groups	1003:1018	Biocompatibility of the fabricated scaffold increased significantly by incorporating curcumin in the scaffold fibers, where protein adsorption, cell attachment, and viability were increased in the nanofiber/curcumin group compared with the other groups.
32307668	5	16	theme	scaffold	868:875	arg1	fibers					877:882	the scaffold fibers	864:882	the scaffold fibers	864:882	Biocompatibility of the fabricated scaffold increased significantly by incorporating curcumin in the scaffold fibers, where protein adsorption, cell attachment, and viability were increased in the nanofiber/curcumin group compared with the other groups.
32307668	6	17	theme	stem	1201:1204	arg1	cells					1206:1210	the stem cells	1197:1210	the stem cells	1197:1210	In addition, the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells upregulated while cultured in the presence of curcumin, but this increase was significantly improved while cells cultured on the nanofibers/curcumin.
32307668	7	18	from	protein	1380:1386	arg1	cells					1395:1399	the cells	1391:1399	the cells cultured on the nanofibers/curcumin	1391:1435	In addition, αSMA protein in the cells cultured on the nanofibers/curcumin expressed significantly higher than those cells cultured on the nanofibers without curcumin.
32307668	2	19	theme	disorders	394:402	arg1	treatment					375:383	the treatment	371:383	the treatment of these disorders	371:402	Tissue engineering in the last two decades has given great hope for the treatment of these disorders.
32307668	5	20	theme	fabricated	791:800	arg1	scaffold					802:809	the fabricated scaffold	787:809	the fabricated scaffold	787:809	Biocompatibility of the fabricated scaffold increased significantly by incorporating curcumin in the scaffold fibers, where protein adsorption, cell attachment, and viability were increased in the nanofiber/curcumin group compared with the other groups.
32307668	6	21	theme	smooth	1058:1063	arg1	Calponin1in					1185:1195	Calponin1in	1185:1195	Calponin1in	1185:1195	In addition, the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells upregulated while cultured in the presence of curcumin, but this increase was significantly improved while cells cultured on the nanofibers/curcumin.
32307668	6	21	theme	smooth	1058:1063	arg1	Caldesmon1					1169:1178	Caldesmon1	1169:1178	Caldesmon1	1169:1178	In addition, the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells upregulated while cultured in the presence of curcumin, but this increase was significantly improved while cells cultured on the nanofibers/curcumin.
32307668	6	21	theme	smooth	1058:1063	arg1	actin					1122:1126	alpha-smooth muscle actin	1102:1126	alpha-smooth muscle actin (αSMA)	1102:1133	In addition, the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells upregulated while cultured in the presence of curcumin, but this increase was significantly improved while cells cultured on the nanofibers/curcumin.
32307668	6	21	theme	smooth	1058:1063	arg1	genes					1085:1089	smooth muscle cell-related genes	1058:1089	smooth muscle cell-related genes	1058:1089	In addition, the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells upregulated while cultured in the presence of curcumin, but this increase was significantly improved while cells cultured on the nanofibers/curcumin.
32307668	6	21	theme	smooth	1058:1063	arg1	muscle					1143:1148	smooth muscle 22 alpha (SM-22a)	1136:1166	smooth muscle 22 alpha (SM-22a)	1136:1166	In addition, the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells upregulated while cultured in the presence of curcumin, but this increase was significantly improved while cells cultured on the nanofibers/curcumin.
32307668	6	22	theme	genes	1085:1089	arg1	level					1049:1053	the expression level	1034:1053	the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells	1034:1210	In addition, the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells upregulated while cultured in the presence of curcumin, but this increase was significantly improved while cells cultured on the nanofibers/curcumin.
32307668	6	23	theme	smooth	1136:1141	arg1	muscle					1143:1148	smooth muscle 22 alpha (SM-22a)	1136:1166	smooth muscle 22 alpha (SM-22a)	1136:1166	In addition, the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells upregulated while cultured in the presence of curcumin, but this increase was significantly improved while cells cultured on the nanofibers/curcumin.
32307668	3	24	theme	nanofibrous	432:442	arg1	scaffold					444:451	a composite nanofibrous scaffold	420:451	a composite nanofibrous scaffold	420:451	In this study, a composite nanofibrous scaffold was fabricated from chitosan, collagen, and polyvinyl-alcohol polymer blend while curcumin incorporated in scaffold fibers.
32307668	0	25	theme	smooth	10:15	arg1	differentiation					29:43	Efficient smooth muscle cell differentiation	0:43	Efficient smooth muscle cell differentiation of iPS cells on curcumin-incorporated chitosan/collagen/polyvinyl-alcohol nanofibers.	0:129	Efficient smooth muscle cell differentiation of iPS cells on curcumin-incorporated chitosan/collagen/polyvinyl-alcohol nanofibers.
32307668	0	26	from	differentiation	29:43	arg1	nanofibers					119:128	curcumin-incorporated chitosan/collagen/polyvinyl-alcohol nanofibers	61:128	curcumin-incorporated chitosan/collagen/polyvinyl-alcohol nanofibers	61:128	Efficient smooth muscle cell differentiation of iPS cells on curcumin-incorporated chitosan/collagen/polyvinyl-alcohol nanofibers.
32307668	4	27	from	differentiation	635:649	arg1	functions					601:609	The scaffold supportive functions	577:609	The scaffold supportive functions from smooth muscle cell differentiation	577:649	The scaffold supportive functions from smooth muscle cell differentiation were studied when human-induced pluripotent stem cells were cultured on the scaffolds under differentiation medium.
32307668	2	28	theme	last	329:332	arg1	decades					338:344	the last two decades	325:344	the last two decades	325:344	Tissue engineering in the last two decades has given great hope for the treatment of these disorders.
32307668	8	29	theme	muscle	1562:1567	arg1	differentiation					1574:1588	smooth muscle cell differentiation	1555:1588	smooth muscle cell differentiation of the induced pluripotent stem cells promoted by curcumin and this promotion	1555:1666	It can be concluded that smooth muscle cell differentiation of the induced pluripotent stem cells promoted by curcumin and this promotion was synergistically improved while curcumin incorporated in the nanofibers.
32307668	0	30	theme	Efficient	0:8	arg1	differentiation					29:43	Efficient smooth muscle cell differentiation	0:43	Efficient smooth muscle cell differentiation of iPS cells on curcumin-incorporated chitosan/collagen/polyvinyl-alcohol nanofibers.	0:129	Efficient smooth muscle cell differentiation of iPS cells on curcumin-incorporated chitosan/collagen/polyvinyl-alcohol nanofibers.
32307668	1	31	theme	recovery	285:292	arg1	process					294:300	its recovery process	281:300	its recovery process	281:300	Bladder dysfunction is one of the most common diseases that occur for a number of reasons and the current treatment modalities do not improve much in its recovery process.
32307668	1	32	theme	Bladder	131:137	arg1	dysfunction					139:149	Bladder dysfunction	131:149	Bladder dysfunction	131:149	Bladder dysfunction is one of the most common diseases that occur for a number of reasons and the current treatment modalities do not improve much in its recovery process.
32307668	0	33	theme	cell	24:27	arg1	differentiation					29:43	Efficient smooth muscle cell differentiation	0:43	Efficient smooth muscle cell differentiation of iPS cells on curcumin-incorporated chitosan/collagen/polyvinyl-alcohol nanofibers.	0:129	Efficient smooth muscle cell differentiation of iPS cells on curcumin-incorporated chitosan/collagen/polyvinyl-alcohol nanofibers.
32307668	3	34	theme	scaffold	560:567	arg1	fibers					569:574	scaffold fibers	560:574	scaffold fibers	560:574	In this study, a composite nanofibrous scaffold was fabricated from chitosan, collagen, and polyvinyl-alcohol polymer blend while curcumin incorporated in scaffold fibers.
32307668	6	35	dep	muscle	1143:1148	arg1	SM-22a					1160:1165	SM-22a	1160:1165	SM-22a	1160:1165	In addition, the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells upregulated while cultured in the presence of curcumin, but this increase was significantly improved while cells cultured on the nanofibers/curcumin.
32307668	6	35	dep	muscle	1143:1148	arg1	alpha					1153:1157	alpha	1153:1157	smooth muscle 22 alpha (SM-22a)	1136:1166	In addition, the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells upregulated while cultured in the presence of curcumin, but this increase was significantly improved while cells cultured on the nanofibers/curcumin.
32307668	1	36	theme	reasons	213:219	arg1	number					203:208	a number	201:208	a number of reasons	201:219	Bladder dysfunction is one of the most common diseases that occur for a number of reasons and the current treatment modalities do not improve much in its recovery process.
32307668	0	37	theme	muscle	17:22	arg1	differentiation					29:43	Efficient smooth muscle cell differentiation	0:43	Efficient smooth muscle cell differentiation of iPS cells on curcumin-incorporated chitosan/collagen/polyvinyl-alcohol nanofibers.	0:129	Efficient smooth muscle cell differentiation of iPS cells on curcumin-incorporated chitosan/collagen/polyvinyl-alcohol nanofibers.
32307668	8	38	theme	stem	1617:1620	arg1	cells					1622:1626	the induced pluripotent stem cells	1593:1626	the induced pluripotent stem cells	1593:1626	It can be concluded that smooth muscle cell differentiation of the induced pluripotent stem cells promoted by curcumin and this promotion was synergistically improved while curcumin incorporated in the nanofibers.
32307668	4	39	theme	muscle	623:628	arg1	differentiation					635:649	smooth muscle cell differentiation	616:649	smooth muscle cell differentiation	616:649	The scaffold supportive functions from smooth muscle cell differentiation were studied when human-induced pluripotent stem cells were cultured on the scaffolds under differentiation medium.
32307668	5	40	dep	increased	947:955	arg1	compared					989:996	compared	989:996	compared with the other groups	989:1018	Biocompatibility of the fabricated scaffold increased significantly by incorporating curcumin in the scaffold fibers, where protein adsorption, cell attachment, and viability were increased in the nanofiber/curcumin group compared with the other groups.
32307668	3	41	theme	polymer	515:521	arg1	blend					523:527	polyvinyl-alcohol polymer blend	497:527	polyvinyl-alcohol polymer blend	497:527	In this study, a composite nanofibrous scaffold was fabricated from chitosan, collagen, and polyvinyl-alcohol polymer blend while curcumin incorporated in scaffold fibers.
32307668	9	42	dep	abstract	1754:1761	arg1	Graphical					1744:1752	Graphical	1744:1752	Graphical	1744:1752	Graphical abstract.
32307668	8	43	theme	pluripotent	1605:1615	arg1	cells					1622:1626	the induced pluripotent stem cells	1593:1626	the induced pluripotent stem cells	1593:1626	It can be concluded that smooth muscle cell differentiation of the induced pluripotent stem cells promoted by curcumin and this promotion was synergistically improved while curcumin incorporated in the nanofibers.
32307668	6	44	theme	alpha-smooth	1102:1113	arg1	αSMA					1129:1132	αSMA	1129:1132	αSMA	1129:1132	In addition, the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells upregulated while cultured in the presence of curcumin, but this increase was significantly improved while cells cultured on the nanofibers/curcumin.
32307668	6	44	theme	alpha-smooth	1102:1113	arg1	actin					1122:1126	alpha-smooth muscle actin	1102:1126	alpha-smooth muscle actin (αSMA)	1102:1133	In addition, the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells upregulated while cultured in the presence of curcumin, but this increase was significantly improved while cells cultured on the nanofibers/curcumin.
32307668	5	45	theme	scaffold	802:809	arg1	Biocompatibility					767:782	Biocompatibility	767:782	Biocompatibility of the fabricated scaffold	767:809	Biocompatibility of the fabricated scaffold increased significantly by incorporating curcumin in the scaffold fibers, where protein adsorption, cell attachment, and viability were increased in the nanofiber/curcumin group compared with the other groups.
32307668	4	46	theme	human-induced	669:681	arg1	cells					700:704	human-induced pluripotent stem cells	669:704	human-induced pluripotent stem cells	669:704	The scaffold supportive functions from smooth muscle cell differentiation were studied when human-induced pluripotent stem cells were cultured on the scaffolds under differentiation medium.
32307668	3	47	theme	composite	422:430	arg1	scaffold					444:451	a composite nanofibrous scaffold	420:451	a composite nanofibrous scaffold	420:451	In this study, a composite nanofibrous scaffold was fabricated from chitosan, collagen, and polyvinyl-alcohol polymer blend while curcumin incorporated in scaffold fibers.
32307668	6	48	theme	curcumin	1258:1265	arg1	presence					1246:1253	the presence	1242:1253	the presence of curcumin	1242:1265	In addition, the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells upregulated while cultured in the presence of curcumin, but this increase was significantly improved while cells cultured on the nanofibers/curcumin.
32307668	1	49	theme	current	229:235	arg1	modalities					247:256	the current treatment modalities	225:256	the current treatment modalities	225:256	Bladder dysfunction is one of the most common diseases that occur for a number of reasons and the current treatment modalities do not improve much in its recovery process.
32307668	0	50	theme	cells	52:56	arg1	differentiation					29:43	Efficient smooth muscle cell differentiation	0:43	Efficient smooth muscle cell differentiation of iPS cells on curcumin-incorporated chitosan/collagen/polyvinyl-alcohol nanofibers.	0:129	Efficient smooth muscle cell differentiation of iPS cells on curcumin-incorporated chitosan/collagen/polyvinyl-alcohol nanofibers.
32307668	8	51	theme	cells	1622:1626	arg1	differentiation					1574:1588	smooth muscle cell differentiation	1555:1588	smooth muscle cell differentiation of the induced pluripotent stem cells promoted by curcumin and this promotion	1555:1666	It can be concluded that smooth muscle cell differentiation of the induced pluripotent stem cells promoted by curcumin and this promotion was synergistically improved while curcumin incorporated in the nanofibers.
32307668	4	52	theme	differentiation	743:757	arg1	medium					759:764	differentiation medium	743:764	differentiation medium	743:764	The scaffold supportive functions from smooth muscle cell differentiation were studied when human-induced pluripotent stem cells were cultured on the scaffolds under differentiation medium.
32307668	0	53	theme	iPS	48:50	arg1	cells					52:56	iPS cells	48:56	iPS cells	48:56	Efficient smooth muscle cell differentiation of iPS cells on curcumin-incorporated chitosan/collagen/polyvinyl-alcohol nanofibers.
32307668	8	54	theme	induced	1597:1603	arg1	cells					1622:1626	the induced pluripotent stem cells	1593:1626	the induced pluripotent stem cells	1593:1626	It can be concluded that smooth muscle cell differentiation of the induced pluripotent stem cells promoted by curcumin and this promotion was synergistically improved while curcumin incorporated in the nanofibers.
32307668	1	55	theme	treatment	237:245	arg1	modalities					247:256	the current treatment modalities	225:256	the current treatment modalities	225:256	Bladder dysfunction is one of the most common diseases that occur for a number of reasons and the current treatment modalities do not improve much in its recovery process.
32307668	4	56	theme	smooth	616:621	arg1	differentiation					635:649	smooth muscle cell differentiation	616:649	smooth muscle cell differentiation	616:649	The scaffold supportive functions from smooth muscle cell differentiation were studied when human-induced pluripotent stem cells were cultured on the scaffolds under differentiation medium.
32307668	0	57	theme	curcumin-incorporated	61:81	arg1	nanofibers					119:128	curcumin-incorporated chitosan/collagen/polyvinyl-alcohol nanofibers	61:128	curcumin-incorporated chitosan/collagen/polyvinyl-alcohol nanofibers	61:128	Efficient smooth muscle cell differentiation of iPS cells on curcumin-incorporated chitosan/collagen/polyvinyl-alcohol nanofibers.
32307668	5	58	theme	cell	911:914	arg1	attachment					916:925	cell attachment	911:925	cell attachment	911:925	Biocompatibility of the fabricated scaffold increased significantly by incorporating curcumin in the scaffold fibers, where protein adsorption, cell attachment, and viability were increased in the nanofiber/curcumin group compared with the other groups.
32307668	7	59	theme	αSMA	1375:1378	arg1	protein					1380:1386	αSMA protein	1375:1386	αSMA protein in the cells cultured on the nanofibers/curcumin	1375:1435	In addition, αSMA protein in the cells cultured on the nanofibers/curcumin expressed significantly higher than those cells cultured on the nanofibers without curcumin.
32307668	5	60	theme	protein	891:897	arg1	adsorption					899:908	protein adsorption	891:908	protein adsorption	891:908	Biocompatibility of the fabricated scaffold increased significantly by incorporating curcumin in the scaffold fibers, where protein adsorption, cell attachment, and viability were increased in the nanofiber/curcumin group compared with the other groups.
32307668	1	61	theme	common	170:175	arg1	diseases					177:184	the most common diseases	161:184	the most common diseases that occur for a number of reasons	161:219	Bladder dysfunction is one of the most common diseases that occur for a number of reasons and the current treatment modalities do not improve much in its recovery process.
32307668	4	62	theme	pluripotent	683:693	arg1	cells					700:704	human-induced pluripotent stem cells	669:704	human-induced pluripotent stem cells	669:704	The scaffold supportive functions from smooth muscle cell differentiation were studied when human-induced pluripotent stem cells were cultured on the scaffolds under differentiation medium.
32307668	6	63	theme	cell-related	1072:1083	arg1	Calponin1in					1185:1195	Calponin1in	1185:1195	Calponin1in	1185:1195	In addition, the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells upregulated while cultured in the presence of curcumin, but this increase was significantly improved while cells cultured on the nanofibers/curcumin.
32307668	6	63	theme	cell-related	1072:1083	arg1	Caldesmon1					1169:1178	Caldesmon1	1169:1178	Caldesmon1	1169:1178	In addition, the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells upregulated while cultured in the presence of curcumin, but this increase was significantly improved while cells cultured on the nanofibers/curcumin.
32307668	6	63	theme	cell-related	1072:1083	arg1	actin					1122:1126	alpha-smooth muscle actin	1102:1126	alpha-smooth muscle actin (αSMA)	1102:1133	In addition, the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells upregulated while cultured in the presence of curcumin, but this increase was significantly improved while cells cultured on the nanofibers/curcumin.
32307668	6	63	theme	cell-related	1072:1083	arg1	genes					1085:1089	smooth muscle cell-related genes	1058:1089	smooth muscle cell-related genes	1058:1089	In addition, the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells upregulated while cultured in the presence of curcumin, but this increase was significantly improved while cells cultured on the nanofibers/curcumin.
32307668	6	63	theme	cell-related	1072:1083	arg1	muscle					1143:1148	smooth muscle 22 alpha (SM-22a)	1136:1166	smooth muscle 22 alpha (SM-22a)	1136:1166	In addition, the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells upregulated while cultured in the presence of curcumin, but this increase was significantly improved while cells cultured on the nanofibers/curcumin.
32307668	6	64	theme	muscle	1065:1070	arg1	Calponin1in					1185:1195	Calponin1in	1185:1195	Calponin1in	1185:1195	In addition, the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells upregulated while cultured in the presence of curcumin, but this increase was significantly improved while cells cultured on the nanofibers/curcumin.
32307668	6	64	theme	muscle	1065:1070	arg1	Caldesmon1					1169:1178	Caldesmon1	1169:1178	Caldesmon1	1169:1178	In addition, the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells upregulated while cultured in the presence of curcumin, but this increase was significantly improved while cells cultured on the nanofibers/curcumin.
32307668	6	64	theme	muscle	1065:1070	arg1	actin					1122:1126	alpha-smooth muscle actin	1102:1126	alpha-smooth muscle actin (αSMA)	1102:1133	In addition, the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells upregulated while cultured in the presence of curcumin, but this increase was significantly improved while cells cultured on the nanofibers/curcumin.
32307668	6	64	theme	muscle	1065:1070	arg1	genes					1085:1089	smooth muscle cell-related genes	1058:1089	smooth muscle cell-related genes	1058:1089	In addition, the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells upregulated while cultured in the presence of curcumin, but this increase was significantly improved while cells cultured on the nanofibers/curcumin.
32307668	6	64	theme	muscle	1065:1070	arg1	muscle					1143:1148	smooth muscle 22 alpha (SM-22a)	1136:1166	smooth muscle 22 alpha (SM-22a)	1136:1166	In addition, the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells upregulated while cultured in the presence of curcumin, but this increase was significantly improved while cells cultured on the nanofibers/curcumin.
32307668	1	65	theme	diseases	177:184	arg1	diseases					177:184	the most common diseases	161:184	the most common diseases that occur for a number of reasons	161:219	Bladder dysfunction is one of the most common diseases that occur for a number of reasons and the current treatment modalities do not improve much in its recovery process.
32307668	1	65	theme	diseases	177:184	arg1	one					154:156	one	154:156	one	154:156	Bladder dysfunction is one of the most common diseases that occur for a number of reasons and the current treatment modalities do not improve much in its recovery process.
32307668	4	66	theme	cell	630:633	arg1	differentiation					635:649	smooth muscle cell differentiation	616:649	smooth muscle cell differentiation	616:649	The scaffold supportive functions from smooth muscle cell differentiation were studied when human-induced pluripotent stem cells were cultured on the scaffolds under differentiation medium.
33442976	3	0	theme	opal	313:316	arg1	film					318:321	The inverse opal film	301:321	The inverse opal film	301:321	The inverse opal film is prepared by using poly(lactic-co-glycolic acid) to negatively replicate a colloidal crystal template, which presents a high specific surface area and interconnected nanopores.
33442976	5	1	theme	film	754:757	arg1	change					740:745	the structure color change	720:745	the structure color change of the film	720:757	In addition, it is demonstrated that the structure color change of the film could intuitively reflect the drug release progress from the nanopores, which made the film a real-time drug monitoring system.
33442976	6	2	from	certified	990:998	arg1	model					909:913	the affected wound model	890:913	the affected wound model	890:913	In the affected wound model, the properties of the multifunctional film in promoting wound healing are certified by the faster healing speed, more granulation tissue, less inflammation, and even a distribution of new blood vessels and collagen.
33442976	0	3	with	Film	39:42	arg1	Multiactives					49:60	Multiactives	49:60	Multiactives	49:60	Multifunctional Composite Inverse Opal Film with Multiactives for Wound Healing.
33442976	7	4	theme	practical	1201:1209	arg1	value					1223:1227	a practical application value	1199:1227	a practical application value	1199:1227	These results indicate that the resultant multifunctional film has a practical application value in clinical wound care.
33442976	6	5	theme	affected	894:901	arg1	model					909:913	the affected wound model	890:913	the affected wound model	890:913	In the affected wound model, the properties of the multifunctional film in promoting wound healing are certified by the faster healing speed, more granulation tissue, less inflammation, and even a distribution of new blood vessels and collagen.
33442976	1	6	theme	wound	162:166	arg1	healing					168:174	wound healing	162:174	wound healing	162:174	A film with an elaborate microstructure and multifunctions is urgently needed in wound healing.
33442976	6	7	theme	wound	972:976	arg1	healing					978:984	wound healing	972:984	wound healing	972:984	In the affected wound model, the properties of the multifunctional film in promoting wound healing are certified by the faster healing speed, more granulation tissue, less inflammation, and even a distribution of new blood vessels and collagen.
33442976	2	8	theme	wound	286:290	arg1	healing					292:298	chronic wound healing	278:298	chronic wound healing	278:298	Here, we present a multiactive encapsulated inverse opal film with a monitorable delivery system for chronic wound healing.
33442976	7	9	contain	has	1195:1197	arg1	film					1190:1193	the resultant multifunctional film	1160:1193	the resultant multifunctional film	1160:1193	These results indicate that the resultant multifunctional film has a practical application value in clinical wound care.
33442976	7	9	contain	has	1195:1197	arg2	value					1223:1227	a practical application value	1199:1227	a practical application value	1199:1227	These results indicate that the resultant multifunctional film has a practical application value in clinical wound care.
33442976	5	10	theme	structure	724:732	arg1	change					740:745	the structure color change	720:745	the structure color change of the film	720:757	In addition, it is demonstrated that the structure color change of the film could intuitively reflect the drug release progress from the nanopores, which made the film a real-time drug monitoring system.
33442976	7	11	theme	application	1211:1221	arg1	value					1223:1227	a practical application value	1199:1227	a practical application value	1199:1227	These results indicate that the resultant multifunctional film has a practical application value in clinical wound care.
33442976	2	12	theme	chronic	278:284	arg1	healing					292:298	chronic wound healing	278:298	chronic wound healing	278:298	Here, we present a multiactive encapsulated inverse opal film with a monitorable delivery system for chronic wound healing.
33442976	7	13	theme	wound	1241:1245	arg1	care					1247:1250	clinical wound care	1232:1250	clinical wound care	1232:1250	These results indicate that the resultant multifunctional film has a practical application value in clinical wound care.
33442976	5	14	theme	color	734:738	arg1	change					740:745	the structure color change	720:745	the structure color change of the film	720:757	In addition, it is demonstrated that the structure color change of the film could intuitively reflect the drug release progress from the nanopores, which made the film a real-time drug monitoring system.
33442976	1	15	dep	microstructure	106:119	arg1	elaborate					96:104	elaborate	96:104	elaborate	96:104	A film with an elaborate microstructure and multifunctions is urgently needed in wound healing.
33442976	6	16	theme	granulation	1034:1044	arg1	tissue					1046:1051	more granulation tissue	1029:1051	more granulation tissue	1029:1051	In the affected wound model, the properties of the multifunctional film in promoting wound healing are certified by the faster healing speed, more granulation tissue, less inflammation, and even a distribution of new blood vessels and collagen.
33442976	6	17	dep	healing	1014:1020	arg1	distribution					1084:1095	a distribution	1082:1095	a distribution of new blood vessels and collagen	1082:1129	In the affected wound model, the properties of the multifunctional film in promoting wound healing are certified by the faster healing speed, more granulation tissue, less inflammation, and even a distribution of new blood vessels and collagen.
33442976	6	18	theme	collagen	1122:1129	arg1	distribution					1084:1095	a distribution	1082:1095	a distribution of new blood vessels and collagen	1082:1129	In the affected wound model, the properties of the multifunctional film in promoting wound healing are certified by the faster healing speed, more granulation tissue, less inflammation, and even a distribution of new blood vessels and collagen.
33442976	5	19	theme	film	846:849	arg1	system					879:884	the film a real-time drug monitoring system	842:884	the film a real-time drug monitoring system	842:884	In addition, it is demonstrated that the structure color change of the film could intuitively reflect the drug release progress from the nanopores, which made the film a real-time drug monitoring system.
33442976	0	20	theme	Composite	16:24	arg1	Film					39:42	Multifunctional Composite Inverse Opal Film	0:42	Multifunctional Composite Inverse Opal Film with Multiactives for Wound Healing.	0:79	Multifunctional Composite Inverse Opal Film with Multiactives for Wound Healing.
33442976	6	21	theme	film	954:957	arg1	certified					990:998	certified	990:998	certified	990:998	In the affected wound model, the properties of the multifunctional film in promoting wound healing are certified by the faster healing speed, more granulation tissue, less inflammation, and even a distribution of new blood vessels and collagen.
33442976	6	21	theme	film	954:957	arg1	properties					920:929	the properties	916:929	the properties of the multifunctional film in promoting wound healing	916:984	In the affected wound model, the properties of the multifunctional film in promoting wound healing are certified by the faster healing speed, more granulation tissue, less inflammation, and even a distribution of new blood vessels and collagen.
33442976	6	22	from	model	909:913	arg1	certified					990:998	certified	990:998	certified	990:998	In the affected wound model, the properties of the multifunctional film in promoting wound healing are certified by the faster healing speed, more granulation tissue, less inflammation, and even a distribution of new blood vessels and collagen.
33442976	6	22	from	model	909:913	arg1	properties					920:929	the properties	916:929	the properties of the multifunctional film in promoting wound healing	916:984	In the affected wound model, the properties of the multifunctional film in promoting wound healing are certified by the faster healing speed, more granulation tissue, less inflammation, and even a distribution of new blood vessels and collagen.
33442976	3	23	theme	crystal	410:416	arg1	template					418:425	a colloidal crystal template	398:425	a colloidal crystal template	398:425	The inverse opal film is prepared by using poly(lactic-co-glycolic acid) to negatively replicate a colloidal crystal template, which presents a high specific surface area and interconnected nanopores.
33442976	0	24	theme	Multifunctional	0:14	arg1	Film					39:42	Multifunctional Composite Inverse Opal Film	0:42	Multifunctional Composite Inverse Opal Film with Multiactives for Wound Healing.	0:79	Multifunctional Composite Inverse Opal Film with Multiactives for Wound Healing.
33442976	4	25	theme	antibacterial	537:549	arg1	effect					551:556	a potent antibacterial effect	528:556	a potent antibacterial effect	528:556	It could be imparted with a potent antibacterial effect and promote angiogenesis by loading the vascular endothelial growth factor into the nanopores and encapsulating by chitosan.
33442976	0	26	theme	Opal	34:37	arg1	Film					39:42	Multifunctional Composite Inverse Opal Film	0:42	Multifunctional Composite Inverse Opal Film with Multiactives for Wound Healing.	0:79	Multifunctional Composite Inverse Opal Film with Multiactives for Wound Healing.
33442976	6	27	theme	vessels	1110:1116	arg1	distribution					1084:1095	a distribution	1082:1095	a distribution of new blood vessels and collagen	1082:1129	In the affected wound model, the properties of the multifunctional film in promoting wound healing are certified by the faster healing speed, more granulation tissue, less inflammation, and even a distribution of new blood vessels and collagen.
33442976	5	28	theme	real-time	853:861	arg1	monitoring					868:877	a real-time drug monitoring	851:877	the film a real-time drug monitoring system	842:884	In addition, it is demonstrated that the structure color change of the film could intuitively reflect the drug release progress from the nanopores, which made the film a real-time drug monitoring system.
33442976	1	29	with	film	83:86	arg1	multifunctions					125:138	multifunctions	125:138	multifunctions	125:138	A film with an elaborate microstructure and multifunctions is urgently needed in wound healing.
33442976	1	29	with	film	83:86	arg1	microstructure					106:119	an elaborate microstructure	93:119	an elaborate microstructure	93:119	A film with an elaborate microstructure and multifunctions is urgently needed in wound healing.
33442976	4	30	theme	potent	530:535	arg1	effect					551:556	a potent antibacterial effect	528:556	a potent antibacterial effect	528:556	It could be imparted with a potent antibacterial effect and promote angiogenesis by loading the vascular endothelial growth factor into the nanopores and encapsulating by chitosan.
33442976	6	31	theme	blood	1104:1108	arg1	vessels					1110:1116	new blood vessels	1100:1116	new blood vessels	1100:1116	In the affected wound model, the properties of the multifunctional film in promoting wound healing are certified by the faster healing speed, more granulation tissue, less inflammation, and even a distribution of new blood vessels and collagen.
33442976	3	32	theme	high	445:448	arg1	area					467:470	a high specific surface area	443:470	a high specific surface area	443:470	The inverse opal film is prepared by using poly(lactic-co-glycolic acid) to negatively replicate a colloidal crystal template, which presents a high specific surface area and interconnected nanopores.
33442976	0	33	theme	Inverse	26:32	arg1	Film					39:42	Multifunctional Composite Inverse Opal Film	0:42	Multifunctional Composite Inverse Opal Film with Multiactives for Wound Healing.	0:79	Multifunctional Composite Inverse Opal Film with Multiactives for Wound Healing.
33442976	6	34	theme	new	1100:1102	arg1	vessels					1110:1116	new blood vessels	1100:1116	new blood vessels	1100:1116	In the affected wound model, the properties of the multifunctional film in promoting wound healing are certified by the faster healing speed, more granulation tissue, less inflammation, and even a distribution of new blood vessels and collagen.
33442976	3	35	theme	specific	450:457	arg1	area					467:470	a high specific surface area	443:470	a high specific surface area	443:470	The inverse opal film is prepared by using poly(lactic-co-glycolic acid) to negatively replicate a colloidal crystal template, which presents a high specific surface area and interconnected nanopores.
33442976	3	36	theme	colloidal	400:408	arg1	template					418:425	a colloidal crystal template	398:425	a colloidal crystal template	398:425	The inverse opal film is prepared by using poly(lactic-co-glycolic acid) to negatively replicate a colloidal crystal template, which presents a high specific surface area and interconnected nanopores.
33442976	3	37	theme	lactic-co-glycolic	349:366	arg1	poly					344:347	poly	344:347	poly(lactic-co-glycolic acid)	344:372	The inverse opal film is prepared by using poly(lactic-co-glycolic acid) to negatively replicate a colloidal crystal template, which presents a high specific surface area and interconnected nanopores.
33442976	3	37	theme	lactic-co-glycolic	349:366	arg1	acid					368:371	lactic-co-glycolic acid	349:371	lactic-co-glycolic acid	349:371	The inverse opal film is prepared by using poly(lactic-co-glycolic acid) to negatively replicate a colloidal crystal template, which presents a high specific surface area and interconnected nanopores.
33442976	6	38	theme	faster	1007:1012	arg1	healing					1014:1020	the faster healing	1003:1020	the faster healing speed	1003:1026	In the affected wound model, the properties of the multifunctional film in promoting wound healing are certified by the faster healing speed, more granulation tissue, less inflammation, and even a distribution of new blood vessels and collagen.
33442976	6	39	theme	multifunctional	938:952	arg1	film					954:957	the multifunctional film	934:957	the multifunctional film in promoting wound healing	934:984	In the affected wound model, the properties of the multifunctional film in promoting wound healing are certified by the faster healing speed, more granulation tissue, less inflammation, and even a distribution of new blood vessels and collagen.
33442976	2	40	theme	inverse	221:227	arg1	film					234:237	a multiactive encapsulated inverse opal film	194:237	a multiactive encapsulated inverse opal film	194:237	Here, we present a multiactive encapsulated inverse opal film with a monitorable delivery system for chronic wound healing.
33442976	5	41	theme	drug	789:792	arg1	progress					802:809	the drug release progress	785:809	the drug release progress	785:809	In addition, it is demonstrated that the structure color change of the film could intuitively reflect the drug release progress from the nanopores, which made the film a real-time drug monitoring system.
33442976	3	42	theme	inverse	305:311	arg1	film					318:321	The inverse opal film	301:321	The inverse opal film	301:321	The inverse opal film is prepared by using poly(lactic-co-glycolic acid) to negatively replicate a colloidal crystal template, which presents a high specific surface area and interconnected nanopores.
33442976	2	43	theme	multiactive	196:206	arg1	film					234:237	a multiactive encapsulated inverse opal film	194:237	a multiactive encapsulated inverse opal film	194:237	Here, we present a multiactive encapsulated inverse opal film with a monitorable delivery system for chronic wound healing.
33442976	2	44	theme	encapsulated	208:219	arg1	film					234:237	a multiactive encapsulated inverse opal film	194:237	a multiactive encapsulated inverse opal film	194:237	Here, we present a multiactive encapsulated inverse opal film with a monitorable delivery system for chronic wound healing.
33442976	7	45	theme	resultant	1164:1172	arg1	film					1190:1193	the resultant multifunctional film	1160:1193	the resultant multifunctional film	1160:1193	These results indicate that the resultant multifunctional film has a practical application value in clinical wound care.
33442976	3	46	theme	interconnected	476:489	arg1	nanopores					491:499	interconnected nanopores	476:499	interconnected nanopores	476:499	The inverse opal film is prepared by using poly(lactic-co-glycolic acid) to negatively replicate a colloidal crystal template, which presents a high specific surface area and interconnected nanopores.
33442976	2	47	theme	delivery	258:265	arg1	system					267:272	a monitorable delivery system	244:272	a monitorable delivery system for chronic wound healing	244:298	Here, we present a multiactive encapsulated inverse opal film with a monitorable delivery system for chronic wound healing.
33442976	2	48	theme	opal	229:232	arg1	film					234:237	a multiactive encapsulated inverse opal film	194:237	a multiactive encapsulated inverse opal film	194:237	Here, we present a multiactive encapsulated inverse opal film with a monitorable delivery system for chronic wound healing.
33442976	3	49	theme	surface	459:465	arg1	area					467:470	a high specific surface area	443:470	a high specific surface area	443:470	The inverse opal film is prepared by using poly(lactic-co-glycolic acid) to negatively replicate a colloidal crystal template, which presents a high specific surface area and interconnected nanopores.
33442976	4	50	theme	vascular	598:605	arg1	factor					626:631	the vascular endothelial growth factor	594:631	the vascular endothelial growth factor	594:631	It could be imparted with a potent antibacterial effect and promote angiogenesis by loading the vascular endothelial growth factor into the nanopores and encapsulating by chitosan.
33442976	7	51	theme	multifunctional	1174:1188	arg1	film					1190:1193	the resultant multifunctional film	1160:1193	the resultant multifunctional film	1160:1193	These results indicate that the resultant multifunctional film has a practical application value in clinical wound care.
33442976	2	52	theme	monitorable	246:256	arg1	system					267:272	a monitorable delivery system	244:272	a monitorable delivery system for chronic wound healing	244:298	Here, we present a multiactive encapsulated inverse opal film with a monitorable delivery system for chronic wound healing.
33442976	5	53	theme	monitoring	868:877	arg1	system					879:884	the film a real-time drug monitoring system	842:884	the film a real-time drug monitoring system	842:884	In addition, it is demonstrated that the structure color change of the film could intuitively reflect the drug release progress from the nanopores, which made the film a real-time drug monitoring system.
33442976	7	54	theme	clinical	1232:1239	arg1	care					1247:1250	clinical wound care	1232:1250	clinical wound care	1232:1250	These results indicate that the resultant multifunctional film has a practical application value in clinical wound care.
33442976	5	55	theme	drug	863:866	arg1	monitoring					868:877	a real-time drug monitoring	851:877	the film a real-time drug monitoring system	842:884	In addition, it is demonstrated that the structure color change of the film could intuitively reflect the drug release progress from the nanopores, which made the film a real-time drug monitoring system.
33442976	4	56	theme	growth	619:624	arg1	factor					626:631	the vascular endothelial growth factor	594:631	the vascular endothelial growth factor	594:631	It could be imparted with a potent antibacterial effect and promote angiogenesis by loading the vascular endothelial growth factor into the nanopores and encapsulating by chitosan.
33442976	0	57	theme	Wound	66:70	arg1	Healing					72:78	Wound Healing	66:78	Wound Healing	66:78	Multifunctional Composite Inverse Opal Film with Multiactives for Wound Healing.
33442976	6	58	theme	wound	903:907	arg1	model					909:913	the affected wound model	890:913	the affected wound model	890:913	In the affected wound model, the properties of the multifunctional film in promoting wound healing are certified by the faster healing speed, more granulation tissue, less inflammation, and even a distribution of new blood vessels and collagen.
33442976	5	59	theme	release	794:800	arg1	progress					802:809	the drug release progress	785:809	the drug release progress	785:809	In addition, it is demonstrated that the structure color change of the film could intuitively reflect the drug release progress from the nanopores, which made the film a real-time drug monitoring system.
33442976	4	60	theme	endothelial	607:617	arg1	factor					626:631	the vascular endothelial growth factor	594:631	the vascular endothelial growth factor	594:631	It could be imparted with a potent antibacterial effect and promote angiogenesis by loading the vascular endothelial growth factor into the nanopores and encapsulating by chitosan.
33310102	1	0	with	polymer	242:248	arg1	variety					262:268	a wide variety	255:268	a wide variety of applications due to its functional properties such as ease of modification, and biodegradability	255:368	Chitosan is a linear polysaccharide and non-toxic bioactive polymer with a wide variety of applications due to its functional properties such as ease of modification, and biodegradability.
33310102	1	0	with	polymer	242:248	arg1	applications					273:284	applications	273:284	applications due to its functional properties such as ease of modification, and biodegradability	273:368	Chitosan is a linear polysaccharide and non-toxic bioactive polymer with a wide variety of applications due to its functional properties such as ease of modification, and biodegradability.
33310102	8	1	theme	CS/AuNPs	1565:1572	arg1	results					1544:1550	the best cytotoxicity results	1522:1550	the best cytotoxicity results of our Fe3O4@CS/AuNPs	1522:1572	Thereby, the best cytotoxicity results of our Fe3O4@CS/AuNPs were observed in the case of the HCT 116 cell line.
33310102	6	2	dep	MKN45	1185:1189	arg1	i.e.					1180:1183	i.e.	1180:1183	i.e.	1180:1183	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	4	3	theme	magnetite-gold	593:606	arg1	nanoparticles					618:630	magnetite-gold composite nanoparticles	593:630	magnetite-gold composite nanoparticles	593:630	In the next step, magnetite-gold composite nanoparticles were synthesized with spherical shapes and sizes ranging from 20 to 30 nm, using sodium citrate as a natural reducing agent.
33310102	3	4	theme	chitosan	558:565	arg1	layers					567:572	chitosan layers	558:572	chitosan layers	558:572	Then the magnetic nanoparticles were encapsulated by chitosan layers.
33310102	1	5	theme	applications	273:284	arg1	variety					262:268	a wide variety	255:268	a wide variety of applications due to its functional properties such as ease of modification, and biodegradability	255:368	Chitosan is a linear polysaccharide and non-toxic bioactive polymer with a wide variety of applications due to its functional properties such as ease of modification, and biodegradability.
33310102	1	5	theme	applications	273:284	arg1	applications					273:284	applications	273:284	applications due to its functional properties such as ease of modification, and biodegradability	273:368	Chitosan is a linear polysaccharide and non-toxic bioactive polymer with a wide variety of applications due to its functional properties such as ease of modification, and biodegradability.
33310102	6	6	dep	lines	1085:1089	arg1	[HRT-18					1118:1124	HCT-8 [HRT-18	1112:1124	HCT-8 [HRT-18	1112:1124	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	6	7	theme	cell	1169:1172	arg1	lines					1174:1178	human gastric cancer cell lines	1148:1178	human gastric cancer cell lines i.e. MKN45	1148:1189	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	5	8	theme	ICP	882:884	arg1	analysis					886:893	ICP analysis	882:893	ICP analysis	882:893	The morphological and physicochemical features of the material were determined using several advanced techniques like FT-IR, ICP analysis, FESEM, EDS, XRD, TEM, XPS and VSM.
33310102	7	9	theme	CS/AuNPs	1305:1312	arg1	IC50					1291:1294	The IC50	1287:1294	The IC50 of Fe3O4@CS/AuNPs	1287:1312	The IC50 of Fe3O4@CS/AuNPs against Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, HT-29, MKN45, AGS, KATO III, PANC-1, AsPC-1, and MIA PaCa-2 cell lines were 385, 429, 264, 286, 442, 498, 561, 513, 528, and 425 μg/mL, respectively.
33310102	7	9	theme	CS/AuNPs	1305:1312	arg1	385					1439:1441	385	1439:1441	385	1439:1441	The IC50 of Fe3O4@CS/AuNPs against Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, HT-29, MKN45, AGS, KATO III, PANC-1, AsPC-1, and MIA PaCa-2 cell lines were 385, 429, 264, 286, 442, 498, 561, 513, 528, and 425 μg/mL, respectively.
33310102	0	10	theme	cancer	147:152	arg1	Investigation					91:103	Investigation	91:103	In situ decorated Au NPs on chitosan-encapsulated Fe3O4-NH2 NPs as magnetic nanocomposite: Investigation of its anti-colon carcinoma, anti-gastric cancer and anti-pancreatic cancer.	0:180	In situ decorated Au NPs on chitosan-encapsulated Fe3O4-NH2 NPs as magnetic nanocomposite: Investigation of its anti-colon carcinoma, anti-gastric cancer and anti-pancreatic cancer.
33310102	6	11	dep	Ramos.2G6.4C10	1096:1109	arg1	i.e.					1091:1094	i.e.	1091:1094	i.e.	1091:1094	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	6	12	theme	gastric	1154:1160	arg1	lines					1174:1178	human gastric cancer cell lines	1148:1178	human gastric cancer cell lines i.e. MKN45	1148:1189	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	6	13	theme	biological	938:947	arg1	part					949:952	the biological part	934:952	the biological part of the present study	934:973	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	4	14	theme	spherical	654:662	arg1	shapes					664:669	spherical shapes	654:669	spherical shapes	654:669	In the next step, magnetite-gold composite nanoparticles were synthesized with spherical shapes and sizes ranging from 20 to 30 nm, using sodium citrate as a natural reducing agent.
33310102	6	15	theme	human	1215:1219	arg1	cancer					1232:1237	human pancreatic cancer	1215:1237	human pancreatic cancer	1215:1237	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	6	16	theme	human	1148:1152	arg1	lines					1174:1178	human gastric cancer cell lines	1148:1178	human gastric cancer cell lines i.e. MKN45	1148:1189	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	8	17	theme	cell	1615:1618	arg1	line					1620:1623	the HCT 116 cell line	1603:1623	the HCT 116 cell line	1603:1623	Thereby, the best cytotoxicity results of our Fe3O4@CS/AuNPs were observed in the case of the HCT 116 cell line.
33310102	1	18	theme	non-toxic	222:230	arg1	polymer					242:248	non-toxic bioactive polymer	222:248	non-toxic bioactive polymer	222:248	Chitosan is a linear polysaccharide and non-toxic bioactive polymer with a wide variety of applications due to its functional properties such as ease of modification, and biodegradability.
33310102	7	19	theme	PaCa-2	1416:1421	arg1	lines					1428:1432	MIA PaCa-2 cell lines	1412:1432	MIA PaCa-2 cell lines	1412:1432	The IC50 of Fe3O4@CS/AuNPs against Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, HT-29, MKN45, AGS, KATO III, PANC-1, AsPC-1, and MIA PaCa-2 cell lines were 385, 429, 264, 286, 442, 498, 561, 513, 528, and 425 μg/mL, respectively.
33310102	6	20	theme	HT-29	1141:1145	arg1	lines					1244:1248	HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines	1141:1248	HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines	1141:1248	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	6	21	theme	KATO	1201:1204	arg1	lines					1244:1248	HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines	1141:1248	HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines	1141:1248	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	8	22	theme	HCT	1607:1609	arg1	line					1620:1623	the HCT 116 cell line	1603:1623	the HCT 116 cell line	1603:1623	Thereby, the best cytotoxicity results of our Fe3O4@CS/AuNPs were observed in the case of the HCT 116 cell line.
33310102	0	23	theme	anti-pancreatic	158:172	arg1	cancer					174:179	anti-pancreatic cancer	158:179	anti-pancreatic cancer	158:179	In situ decorated Au NPs on chitosan-encapsulated Fe3O4-NH2 NPs as magnetic nanocomposite: Investigation of its anti-colon carcinoma, anti-gastric cancer and anti-pancreatic cancer.
33310102	6	24	theme	cell	980:983	arg1	low					1041:1043	low	1041:1043	low	1041:1043	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	6	24	theme	cell	980:983	arg1	viability					985:993	the cell viability	976:993	the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs	976:1030	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	5	25	theme	morphological	761:773	arg1	features					795:802	The morphological and physicochemical features	757:802	The morphological and physicochemical features of the material	757:818	The morphological and physicochemical features of the material were determined using several advanced techniques like FT-IR, ICP analysis, FESEM, EDS, XRD, TEM, XPS and VSM.
33310102	5	26	theme	several	842:848	arg1	techniques					859:868	several advanced techniques	842:868	several advanced techniques like FT-IR, ICP analysis, FESEM, EDS, XRD, TEM, XPS and VSM	842:928	The morphological and physicochemical features of the material were determined using several advanced techniques like FT-IR, ICP analysis, FESEM, EDS, XRD, TEM, XPS and VSM.
33310102	0	27	theme	magnetic	67:74	arg1	nanocomposite					76:88	magnetic nanocomposite	67:88	magnetic nanocomposite	67:88	In situ decorated Au NPs on chitosan-encapsulated Fe3O4-NH2 NPs as magnetic nanocomposite: Investigation of its anti-colon carcinoma, anti-gastric cancer and anti-pancreatic cancer.
33310102	8	28	theme	best	1526:1529	arg1	results					1544:1550	the best cytotoxicity results	1522:1550	the best cytotoxicity results of our Fe3O4@CS/AuNPs	1522:1572	Thereby, the best cytotoxicity results of our Fe3O4@CS/AuNPs were observed in the case of the HCT 116 cell line.
33310102	6	29	theme	present	961:967	arg1	study					969:973	the present study	957:973	the present study	957:973	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	9	30	theme	several	1696:1702	arg1	types					1704:1708	several types	1696:1708	several types of gastro-duodenal cancers	1696:1735	Seemingly, the present nanoparticles may be used for the treatment of several types of gastro-duodenal cancers especially colon, gastric, and pancreatic cancers in near future.
33310102	1	31	theme	biodegradability	353:368	arg1	ease					327:330	ease	327:330	ease of modification, and biodegradability	327:368	Chitosan is a linear polysaccharide and non-toxic bioactive polymer with a wide variety of applications due to its functional properties such as ease of modification, and biodegradability.
33310102	6	32	theme	cancer	1232:1237	arg1	lines					1244:1248	HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines	1141:1248	HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines	1141:1248	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	6	33	dep	PANC-1	1255:1260	arg1	PaCa-2					1279:1284	PaCa-2	1279:1284	PaCa-2	1279:1284	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	6	33	dep	PANC-1	1255:1260	arg1	i.e.					1250:1253	i.e.	1250:1253	i.e.	1250:1253	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	6	34	theme	Fe3O4	1017:1021	arg1	CS/AuNPs					1023:1030	Fe3O4@CS/AuNPs	1017:1030	Fe3O4@CS/AuNPs	1017:1030	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	2	35	theme	fabrication	442:452	arg1	method					454:459	a fabrication method	440:459	a fabrication method involving coprecipitation of Fe2+ and Fe3+	440:502	In this investigation, magnetic cores (Fe3O4) were synthesized using a fabrication method involving coprecipitation of Fe2+ and Fe3+.
33310102	0	36	from	NPs	21:23	arg1	NPs					60:62	chitosan-encapsulated Fe3O4-NH2 NPs	28:62	chitosan-encapsulated Fe3O4-NH2 NPs	28:62	In situ decorated Au NPs on chitosan-encapsulated Fe3O4-NH2 NPs as magnetic nanocomposite: Investigation of its anti-colon carcinoma, anti-gastric cancer and anti-pancreatic cancer.
33310102	9	37	theme	cancers	1729:1735	arg1	types					1704:1708	several types	1696:1708	several types of gastro-duodenal cancers	1696:1735	Seemingly, the present nanoparticles may be used for the treatment of several types of gastro-duodenal cancers especially colon, gastric, and pancreatic cancers in near future.
33310102	4	38	theme	reducing	741:748	arg1	agent					750:754	a natural reducing agent	731:754	a natural reducing agent	731:754	In the next step, magnetite-gold composite nanoparticles were synthesized with spherical shapes and sizes ranging from 20 to 30 nm, using sodium citrate as a natural reducing agent.
33310102	4	38	theme	reducing	741:748	arg1	citrate					720:726	sodium citrate	713:726	sodium citrate	713:726	In the next step, magnetite-gold composite nanoparticles were synthesized with spherical shapes and sizes ranging from 20 to 30 nm, using sodium citrate as a natural reducing agent.
33310102	0	39	theme	In	0:1	arg1	NPs					21:23	In situ decorated Au NPs	0:23	In situ decorated Au NPs on chitosan-encapsulated Fe3O4-NH2 NPs as magnetic nanocomposite: Investigation of its anti-colon carcinoma, anti-gastric cancer and anti-pancreatic cancer.	0:180	In situ decorated Au NPs on chitosan-encapsulated Fe3O4-NH2 NPs as magnetic nanocomposite: Investigation of its anti-colon carcinoma, anti-gastric cancer and anti-pancreatic cancer.
33310102	1	40	theme	due	286:288	arg1	applications					273:284	applications	273:284	applications due to its functional properties such as ease of modification, and biodegradability	273:368	Chitosan is a linear polysaccharide and non-toxic bioactive polymer with a wide variety of applications due to its functional properties such as ease of modification, and biodegradability.
33310102	8	41	theme	@	1564:1564	arg1	CS/AuNPs					1565:1572	our Fe3O4@CS/AuNPs	1555:1572	our Fe3O4@CS/AuNPs	1555:1572	Thereby, the best cytotoxicity results of our Fe3O4@CS/AuNPs were observed in the case of the HCT 116 cell line.
33310102	0	42	theme	decorated	8:16	arg1	NPs					21:23	In situ decorated Au NPs	0:23	In situ decorated Au NPs on chitosan-encapsulated Fe3O4-NH2 NPs as magnetic nanocomposite: Investigation of its anti-colon carcinoma, anti-gastric cancer and anti-pancreatic cancer.	0:180	In situ decorated Au NPs on chitosan-encapsulated Fe3O4-NH2 NPs as magnetic nanocomposite: Investigation of its anti-colon carcinoma, anti-gastric cancer and anti-pancreatic cancer.
33310102	6	43	from	part	949:952	arg1	low					1041:1043	low	1041:1043	low	1041:1043	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	6	43	from	part	949:952	arg1	viability					985:993	the cell viability	976:993	the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs	976:1030	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	1	44	with	polysaccharide	203:216	arg1	variety					262:268	a wide variety	255:268	a wide variety of applications due to its functional properties such as ease of modification, and biodegradability	255:368	Chitosan is a linear polysaccharide and non-toxic bioactive polymer with a wide variety of applications due to its functional properties such as ease of modification, and biodegradability.
33310102	1	44	with	polysaccharide	203:216	arg1	applications					273:284	applications	273:284	applications due to its functional properties such as ease of modification, and biodegradability	273:368	Chitosan is a linear polysaccharide and non-toxic bioactive polymer with a wide variety of applications due to its functional properties such as ease of modification, and biodegradability.
33310102	5	45	theme	material	811:818	arg1	features					795:802	The morphological and physicochemical features	757:802	The morphological and physicochemical features of the material	757:818	The morphological and physicochemical features of the material were determined using several advanced techniques like FT-IR, ICP analysis, FESEM, EDS, XRD, TEM, XPS and VSM.
33310102	0	46	dep	NPs	21:23	arg1	Investigation					91:103	Investigation	91:103	In situ decorated Au NPs on chitosan-encapsulated Fe3O4-NH2 NPs as magnetic nanocomposite: Investigation of its anti-colon carcinoma, anti-gastric cancer and anti-pancreatic cancer.	0:180	In situ decorated Au NPs on chitosan-encapsulated Fe3O4-NH2 NPs as magnetic nanocomposite: Investigation of its anti-colon carcinoma, anti-gastric cancer and anti-pancreatic cancer.
33310102	6	47	theme	colorectal	1059:1068	arg1	lines					1085:1089	human colorectal carcinoma cell lines	1053:1089	human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18]	1053:1125	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	1	48	theme	bioactive	232:240	arg1	polymer					242:248	non-toxic bioactive polymer	222:248	non-toxic bioactive polymer	222:248	Chitosan is a linear polysaccharide and non-toxic bioactive polymer with a wide variety of applications due to its functional properties such as ease of modification, and biodegradability.
33310102	4	49	theme	composite	608:616	arg1	nanoparticles					618:630	magnetite-gold composite nanoparticles	593:630	magnetite-gold composite nanoparticles	593:630	In the next step, magnetite-gold composite nanoparticles were synthesized with spherical shapes and sizes ranging from 20 to 30 nm, using sodium citrate as a natural reducing agent.
33310102	3	50	theme	magnetic	514:521	arg1	nanoparticles					523:535	the magnetic nanoparticles	510:535	the magnetic nanoparticles	510:535	Then the magnetic nanoparticles were encapsulated by chitosan layers.
33310102	0	51	dep	In	0:1	arg1	situ					3:6	situ	3:6	situ	3:6	In situ decorated Au NPs on chitosan-encapsulated Fe3O4-NH2 NPs as magnetic nanocomposite: Investigation of its anti-colon carcinoma, anti-gastric cancer and anti-pancreatic cancer.
33310102	6	52	theme	AGS	1192:1194	arg1	lines					1244:1248	HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines	1141:1248	HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines	1141:1248	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	9	53	dep	colon	1748:1752	arg1	future					1795:1800	future	1795:1800	future	1795:1800	Seemingly, the present nanoparticles may be used for the treatment of several types of gastro-duodenal cancers especially colon, gastric, and pancreatic cancers in near future.
33310102	1	54	theme	wide	257:260	arg1	variety					262:268	a wide variety	255:268	a wide variety of applications due to its functional properties such as ease of modification, and biodegradability	255:368	Chitosan is a linear polysaccharide and non-toxic bioactive polymer with a wide variety of applications due to its functional properties such as ease of modification, and biodegradability.
33310102	1	54	theme	wide	257:260	arg1	applications					273:284	applications	273:284	applications due to its functional properties such as ease of modification, and biodegradability	273:368	Chitosan is a linear polysaccharide and non-toxic bioactive polymer with a wide variety of applications due to its functional properties such as ease of modification, and biodegradability.
33310102	6	55	theme	CS/AuNPs	1023:1030	arg1	low					1041:1043	low	1041:1043	low	1041:1043	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	6	55	theme	CS/AuNPs	1023:1030	arg1	viability					985:993	the cell viability	976:993	the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs	976:1030	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	4	56	theme	next	582:585	arg1	step					587:590	the next step	578:590	the next step	578:590	In the next step, magnetite-gold composite nanoparticles were synthesized with spherical shapes and sizes ranging from 20 to 30 nm, using sodium citrate as a natural reducing agent.
33310102	7	57	theme	Fe3O4	1299:1303	arg1	CS/AuNPs					1305:1312	Fe3O4@CS/AuNPs	1299:1312	Fe3O4@CS/AuNPs	1299:1312	The IC50 of Fe3O4@CS/AuNPs against Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, HT-29, MKN45, AGS, KATO III, PANC-1, AsPC-1, and MIA PaCa-2 cell lines were 385, 429, 264, 286, 442, 498, 561, 513, 528, and 425 μg/mL, respectively.
33310102	6	58	theme	lines	1174:1178	arg1	lines					1244:1248	HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines	1141:1248	HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines	1141:1248	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	0	59	theme	chitosan-encapsulated	28:48	arg1	NPs					60:62	chitosan-encapsulated Fe3O4-NH2 NPs	28:62	chitosan-encapsulated Fe3O4-NH2 NPs	28:62	In situ decorated Au NPs on chitosan-encapsulated Fe3O4-NH2 NPs as magnetic nanocomposite: Investigation of its anti-colon carcinoma, anti-gastric cancer and anti-pancreatic cancer.
33310102	7	60	theme	@	1304:1304	arg1	CS/AuNPs					1305:1312	Fe3O4@CS/AuNPs	1299:1312	Fe3O4@CS/AuNPs	1299:1312	The IC50 of Fe3O4@CS/AuNPs against Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, HT-29, MKN45, AGS, KATO III, PANC-1, AsPC-1, and MIA PaCa-2 cell lines were 385, 429, 264, 286, 442, 498, 561, 513, 528, and 425 μg/mL, respectively.
33310102	5	61	theme	physicochemical	779:793	arg1	features					795:802	The morphological and physicochemical features	757:802	The morphological and physicochemical features of the material	757:818	The morphological and physicochemical features of the material were determined using several advanced techniques like FT-IR, ICP analysis, FESEM, EDS, XRD, TEM, XPS and VSM.
33310102	6	62	theme	cancer	1162:1167	arg1	lines					1174:1178	human gastric cancer cell lines	1148:1178	human gastric cancer cell lines i.e. MKN45	1148:1189	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	6	63	theme	pancreatic	1221:1230	arg1	cancer					1232:1237	human pancreatic cancer	1215:1237	human pancreatic cancer	1215:1237	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	5	64	theme	advanced	850:857	arg1	techniques					859:868	several advanced techniques	842:868	several advanced techniques like FT-IR, ICP analysis, FESEM, EDS, XRD, TEM, XPS and VSM	842:928	The morphological and physicochemical features of the material were determined using several advanced techniques like FT-IR, ICP analysis, FESEM, EDS, XRD, TEM, XPS and VSM.
33310102	7	65	theme	cell	1423:1426	arg1	lines					1428:1432	MIA PaCa-2 cell lines	1412:1432	MIA PaCa-2 cell lines	1412:1432	The IC50 of Fe3O4@CS/AuNPs against Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, HT-29, MKN45, AGS, KATO III, PANC-1, AsPC-1, and MIA PaCa-2 cell lines were 385, 429, 264, 286, 442, 498, 561, 513, 528, and 425 μg/mL, respectively.
33310102	6	66	theme	cell	1080:1083	arg1	lines					1085:1089	human colorectal carcinoma cell lines	1053:1089	human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18]	1053:1125	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	0	67	theme	anti-gastric	134:145	arg1	cancer					147:152	anti-gastric cancer	134:152	anti-gastric cancer	134:152	In situ decorated Au NPs on chitosan-encapsulated Fe3O4-NH2 NPs as magnetic nanocomposite: Investigation of its anti-colon carcinoma, anti-gastric cancer and anti-pancreatic cancer.
33310102	7	68	theme	MIA	1412:1414	arg1	lines					1428:1432	MIA PaCa-2 cell lines	1412:1432	MIA PaCa-2 cell lines	1412:1432	The IC50 of Fe3O4@CS/AuNPs against Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, HT-29, MKN45, AGS, KATO III, PANC-1, AsPC-1, and MIA PaCa-2 cell lines were 385, 429, 264, 286, 442, 498, 561, 513, 528, and 425 μg/mL, respectively.
33310102	9	69	theme	present	1641:1647	arg1	nanoparticles					1649:1661	the present nanoparticles	1637:1661	the present nanoparticles	1637:1661	Seemingly, the present nanoparticles may be used for the treatment of several types of gastro-duodenal cancers especially colon, gastric, and pancreatic cancers in near future.
33310102	0	70	theme	carcinoma	123:131	arg1	Investigation					91:103	Investigation	91:103	In situ decorated Au NPs on chitosan-encapsulated Fe3O4-NH2 NPs as magnetic nanocomposite: Investigation of its anti-colon carcinoma, anti-gastric cancer and anti-pancreatic cancer.	0:180	In situ decorated Au NPs on chitosan-encapsulated Fe3O4-NH2 NPs as magnetic nanocomposite: Investigation of its anti-colon carcinoma, anti-gastric cancer and anti-pancreatic cancer.
33310102	9	71	used	used	1670:1673	arg2	nanoparticles					1649:1661	the present nanoparticles	1637:1661	the present nanoparticles	1637:1661	Seemingly, the present nanoparticles may be used for the treatment of several types of gastro-duodenal cancers especially colon, gastric, and pancreatic cancers in near future.
33310102	4	72	theme	sodium	713:718	arg1	agent					750:754	a natural reducing agent	731:754	a natural reducing agent	731:754	In the next step, magnetite-gold composite nanoparticles were synthesized with spherical shapes and sizes ranging from 20 to 30 nm, using sodium citrate as a natural reducing agent.
33310102	4	72	theme	sodium	713:718	arg1	citrate					720:726	sodium citrate	713:726	sodium citrate	713:726	In the next step, magnetite-gold composite nanoparticles were synthesized with spherical shapes and sizes ranging from 20 to 30 nm, using sodium citrate as a natural reducing agent.
33310102	0	73	theme	cancer	174:179	arg1	Investigation					91:103	Investigation	91:103	In situ decorated Au NPs on chitosan-encapsulated Fe3O4-NH2 NPs as magnetic nanocomposite: Investigation of its anti-colon carcinoma, anti-gastric cancer and anti-pancreatic cancer.	0:180	In situ decorated Au NPs on chitosan-encapsulated Fe3O4-NH2 NPs as magnetic nanocomposite: Investigation of its anti-colon carcinoma, anti-gastric cancer and anti-pancreatic cancer.
33310102	1	74	theme	linear	196:201	arg1	polysaccharide					203:216	a linear polysaccharide	194:216	a linear polysaccharide	194:216	Chitosan is a linear polysaccharide and non-toxic bioactive polymer with a wide variety of applications due to its functional properties such as ease of modification, and biodegradability.
33310102	1	74	theme	linear	196:201	arg1	Chitosan					182:189	Chitosan	182:189	Chitosan	182:189	Chitosan is a linear polysaccharide and non-toxic bioactive polymer with a wide variety of applications due to its functional properties such as ease of modification, and biodegradability.
33310102	2	75	theme	Fe3+	499:502	arg1	coprecipitation					471:485	coprecipitation	471:485	coprecipitation of Fe2+ and Fe3+	471:502	In this investigation, magnetic cores (Fe3O4) were synthesized using a fabrication method involving coprecipitation of Fe2+ and Fe3+.
33310102	1	76	theme	modification	335:346	arg1	ease					327:330	ease	327:330	ease of modification, and biodegradability	327:368	Chitosan is a linear polysaccharide and non-toxic bioactive polymer with a wide variety of applications due to its functional properties such as ease of modification, and biodegradability.
33310102	6	77	theme	study	969:973	arg1	part					949:952	the biological part	934:952	the biological part of the present study	934:973	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	2	78	theme	Fe2+	490:493	arg1	coprecipitation					471:485	coprecipitation	471:485	coprecipitation of Fe2+ and Fe3+	471:502	In this investigation, magnetic cores (Fe3O4) were synthesized using a fabrication method involving coprecipitation of Fe2+ and Fe3+.
33310102	9	79	theme	types	1704:1708	arg1	treatment					1683:1691	the treatment	1679:1691	the treatment of several types of gastro-duodenal cancers	1679:1735	Seemingly, the present nanoparticles may be used for the treatment of several types of gastro-duodenal cancers especially colon, gastric, and pancreatic cancers in near future.
33310102	6	80	theme	cell	1239:1242	arg1	lines					1244:1248	HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines	1141:1248	HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines	1141:1248	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	8	81	theme	cytotoxicity	1531:1542	arg1	results					1544:1550	the best cytotoxicity results	1522:1550	the best cytotoxicity results of our Fe3O4@CS/AuNPs	1522:1572	Thereby, the best cytotoxicity results of our Fe3O4@CS/AuNPs were observed in the case of the HCT 116 cell line.
33310102	6	82	theme	@	1022:1022	arg1	CS/AuNPs					1023:1030	Fe3O4@CS/AuNPs	1017:1030	Fe3O4@CS/AuNPs	1017:1030	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	9	83	theme	gastro-duodenal	1713:1727	arg1	cancers					1729:1735	gastro-duodenal cancers	1713:1735	gastro-duodenal cancers	1713:1735	Seemingly, the present nanoparticles may be used for the treatment of several types of gastro-duodenal cancers especially colon, gastric, and pancreatic cancers in near future.
33310102	2	84	theme	magnetic	394:401	arg1	Fe3O4					410:414	Fe3O4	410:414	Fe3O4	410:414	In this investigation, magnetic cores (Fe3O4) were synthesized using a fabrication method involving coprecipitation of Fe2+ and Fe3+.
33310102	2	84	theme	magnetic	394:401	arg1	cores					403:407	magnetic cores	394:407	magnetic cores (Fe3O4)	394:415	In this investigation, magnetic cores (Fe3O4) were synthesized using a fabrication method involving coprecipitation of Fe2+ and Fe3+.
33310102	8	85	located	observed	1579:1586	arg1	case					1595:1598	the case	1591:1598	the case of the HCT 116 cell line	1591:1623	Thereby, the best cytotoxicity results of our Fe3O4@CS/AuNPs were observed in the case of the HCT 116 cell line.
33310102	8	85	located	observed	1579:1586	arg2	results					1544:1550	the best cytotoxicity results	1522:1550	the best cytotoxicity results of our Fe3O4@CS/AuNPs	1522:1572	Thereby, the best cytotoxicity results of our Fe3O4@CS/AuNPs were observed in the case of the HCT 116 cell line.
33310102	8	86	theme	line	1620:1623	arg1	case					1595:1598	the case	1591:1598	the case of the HCT 116 cell line	1591:1623	Thereby, the best cytotoxicity results of our Fe3O4@CS/AuNPs were observed in the case of the HCT 116 cell line.
33310102	4	87	theme	natural	733:739	arg1	agent					750:754	a natural reducing agent	731:754	a natural reducing agent	731:754	In the next step, magnetite-gold composite nanoparticles were synthesized with spherical shapes and sizes ranging from 20 to 30 nm, using sodium citrate as a natural reducing agent.
33310102	4	87	theme	natural	733:739	arg1	citrate					720:726	sodium citrate	713:726	sodium citrate	713:726	In the next step, magnetite-gold composite nanoparticles were synthesized with spherical shapes and sizes ranging from 20 to 30 nm, using sodium citrate as a natural reducing agent.
33310102	0	88	theme	Au	18:19	arg1	NPs					21:23	In situ decorated Au NPs	0:23	In situ decorated Au NPs on chitosan-encapsulated Fe3O4-NH2 NPs as magnetic nanocomposite: Investigation of its anti-colon carcinoma, anti-gastric cancer and anti-pancreatic cancer.	0:180	In situ decorated Au NPs on chitosan-encapsulated Fe3O4-NH2 NPs as magnetic nanocomposite: Investigation of its anti-colon carcinoma, anti-gastric cancer and anti-pancreatic cancer.
33310102	8	89	theme	Fe3O4	1559:1563	arg1	CS/AuNPs					1565:1572	our Fe3O4@CS/AuNPs	1555:1572	our Fe3O4@CS/AuNPs	1555:1572	Thereby, the best cytotoxicity results of our Fe3O4@CS/AuNPs were observed in the case of the HCT 116 cell line.
33310102	6	90	theme	HAuCl4	1005:1010	arg1	low					1041:1043	low	1041:1043	low	1041:1043	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	6	90	theme	HAuCl4	1005:1010	arg1	viability					985:993	the cell viability	976:993	the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs	976:1030	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	1	91	theme	functional	297:306	arg1	properties					308:317	its functional properties	293:317	its functional properties such as ease of modification, and biodegradability	293:368	Chitosan is a linear polysaccharide and non-toxic bioactive polymer with a wide variety of applications due to its functional properties such as ease of modification, and biodegradability.
33310102	1	91	theme	functional	297:306	arg1	ease					327:330	ease	327:330	ease of modification, and biodegradability	327:368	Chitosan is a linear polysaccharide and non-toxic bioactive polymer with a wide variety of applications due to its functional properties such as ease of modification, and biodegradability.
33310102	6	92	theme	Fe3O4	998:1002	arg1	low					1041:1043	low	1041:1043	low	1041:1043	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	6	92	theme	Fe3O4	998:1002	arg1	viability					985:993	the cell viability	976:993	the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs	976:1030	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	0	93	theme	Fe3O4-NH2	50:58	arg1	NPs					60:62	chitosan-encapsulated Fe3O4-NH2 NPs	28:62	chitosan-encapsulated Fe3O4-NH2 NPs	28:62	In situ decorated Au NPs on chitosan-encapsulated Fe3O4-NH2 NPs as magnetic nanocomposite: Investigation of its anti-colon carcinoma, anti-gastric cancer and anti-pancreatic cancer.
33310102	6	94	theme	carcinoma	1070:1078	arg1	lines					1085:1089	human colorectal carcinoma cell lines	1053:1089	human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18]	1053:1125	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	9	95	theme	pancreatic	1768:1777	arg1	cancers					1779:1785	pancreatic cancers	1768:1785	pancreatic cancers	1768:1785	Seemingly, the present nanoparticles may be used for the treatment of several types of gastro-duodenal cancers especially colon, gastric, and pancreatic cancers in near future.
33310102	7	96	dep	Ramos.2G6.4C10	1322:1335	arg1	[HRT-18					1344:1350	HCT-8 [HRT-18	1338:1350	HCT-8 [HRT-18	1338:1350	The IC50 of Fe3O4@CS/AuNPs against Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, HT-29, MKN45, AGS, KATO III, PANC-1, AsPC-1, and MIA PaCa-2 cell lines were 385, 429, 264, 286, 442, 498, 561, 513, 528, and 425 μg/mL, respectively.
33310102	6	97	theme	human	1053:1057	arg1	lines					1085:1089	human colorectal carcinoma cell lines	1053:1089	human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18]	1053:1125	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33310102	0	98	theme	anti-colon	112:121	arg1	carcinoma					123:131	its anti-colon carcinoma	108:131	its anti-colon carcinoma	108:131	In situ decorated Au NPs on chitosan-encapsulated Fe3O4-NH2 NPs as magnetic nanocomposite: Investigation of its anti-colon carcinoma, anti-gastric cancer and anti-pancreatic cancer.
33310102	6	99	from	low	1041:1043	arg1	part					949:952	the biological part	934:952	the biological part of the present study	934:973	In the biological part of the present study, the cell viability of Fe3O4, HAuCl4, and Fe3O4@CS/AuNPs was very low against human colorectal carcinoma cell lines i.e. Ramos.2G6.4C10, HCT-8 [HRT-18], HCT 116, and HT-29, human gastric cancer cell lines i.e. MKN45, AGS, and KATO III, and human pancreatic cancer cell lines i.e. PANC-1, AsPC-1, and MIA PaCa-2.
33533929	2	0	from	individuals	584:594	arg1	use					531:533	use	531:533	use of a NOAC vs use of the LMWH enoxaparin in Asian individuals with cancer-associated VTE	531:621	Objective To compare the risk of recurrent thromboembolic events and bleeding associated with use of a NOAC vs use of the LMWH enoxaparin in Asian individuals with cancer-associated VTE.
33533929	11	1	theme	mean	1619:1622	arg1	SD					1625:1626	SD	1625:1626	SD	1625:1626	Results Among 1109 patients with cancer and newly diagnosed VTE, 578 (52.1%) were women and the mean (SD) age at index date was 66.0 (13.0) years; 529 patients (47.7%) received NOACs and 580 patients (52.3%) received the LMWH enoxaparin.
33533929	11	1	theme	mean	1619:1622	arg1	age					1629:1631	the mean (SD) age	1615:1631	the mean (SD) age at index date	1615:1645	Results Among 1109 patients with cancer and newly diagnosed VTE, 578 (52.1%) were women and the mean (SD) age at index date was 66.0 (13.0) years; 529 patients (47.7%) received NOACs and 580 patients (52.3%) received the LMWH enoxaparin.
33533929	15	2	theme	competing	2409:2417	arg1	analyses					2424:2431	competing risk analyses	2409:2431	competing risk analyses	2409:2431	Findings for both primary outcomes were consistent with competing risk analyses (recurrent VTE: HR, 0.68; 95% CI, 0.45-1.01; P = .05; major bleeding: HR, 0.77; 95% CI, 0.51-1.16; P = .21).
33533929	17	3	theme	bleeding	2891:2898	arg1	rate					2866:2869	a significantly lower rate	2844:2869	a significantly lower rate of gastrointestinal bleeding	2844:2898	Nonetheless, use of a NOAC was associated with a significantly lower rate of gastrointestinal bleeding.
33533929	16	4	theme	real-world	2600:2609	arg1	practice					2611:2618	real-world practice	2600:2618	real-world practice	2600:2618	Conclusions and Relevance This cohort study found that in real-world practice, among Asian patients with cancer-associated VTE, use of a NOAC was associated with a similar risk for recurrent VTE or major bleeding compared with use of the LMWH enoxaparin.
33533929	12	5	from	patients	1861:1868	arg1	group					1847:1851	the NOAC group	1838:1851	the NOAC group	1838:1851	Composite recurrent VTE or major bleeding occurred in 75 patients (14.1%) in the NOAC group and 101 patients (17.4%) in the enoxaparin group (weighted hazard ratio [HR], 0.77; 95% CI, 0.56-1.07; P = .11).
33533929	12	6	theme	%	1939:1939	arg1	CI					1941:1942	95% CI	1937:1942	95% CI	1937:1942	Composite recurrent VTE or major bleeding occurred in 75 patients (14.1%) in the NOAC group and 101 patients (17.4%) in the enoxaparin group (weighted hazard ratio [HR], 0.77; 95% CI, 0.56-1.07; P = .11).
33533929	15	7	dep	consistent	2393:2402	arg1	VTE					2444:2446	recurrent VTE	2434:2446	recurrent VTE	2434:2446	Findings for both primary outcomes were consistent with competing risk analyses (recurrent VTE: HR, 0.68; 95% CI, 0.45-1.01; P = .05; major bleeding: HR, 0.77; 95% CI, 0.51-1.16; P = .21).
33533929	1	8	from	heparins	347:354	arg1	individuals					373:383	Asian individuals	367:383	Asian individuals with cancer and acute venous thromboembolism (VTE)	367:434	Importance It is unclear whether the clinical benefits associated with non-vitamin K antagonist oral anticoagulants (NOACs) are similar to those associated with low-molecular-weight heparins (LMWHs) in Asian individuals with cancer and acute venous thromboembolism (VTE).
33533929	8	9	used	used	1247:1250	arg2	probability					1208:1218	Stabilized inverse probability	1189:1218	Stabilized inverse probability of treatment weighting	1189:1241	Stabilized inverse probability of treatment weighting was used to balance baseline covariates.
33533929	8	10	theme	balance	1255:1261	arg1	covariates					1272:1281	balance baseline covariates	1255:1281	balance baseline covariates	1255:1281	Stabilized inverse probability of treatment weighting was used to balance baseline covariates.
33533929	3	11	theme	records	781:787	arg1	database					789:796	a multi-institutional electronic medical records database	740:796	a multi-institutional electronic medical records database in Taiwan	740:806	Design, Setting, and Participants This cohort study was conducted using data from the Chang Gung Research Database, a multi-institutional electronic medical records database in Taiwan.
33533929	3	11	theme	records	781:787	arg1	Database					730:737	the Chang Gung Research Database	706:737	the Chang Gung Research Database	706:737	Design, Setting, and Participants This cohort study was conducted using data from the Chang Gung Research Database, a multi-institutional electronic medical records database in Taiwan.
33533929	10	12	theme	subdistribution	1451:1465	arg1	model					1474:1478	a Fine and Gray subdistribution hazard model	1435:1478	a Fine and Gray subdistribution hazard model that considered death as a competing risk	1435:1520	In addition, we conducted an analysis using a Fine and Gray subdistribution hazard model that considered death as a competing risk.
33533929	16	13	theme	Asian	2627:2631	arg1	patients					2633:2640	Asian patients	2627:2640	Asian patients with cancer-associated VTE	2627:2667	Conclusions and Relevance This cohort study found that in real-world practice, among Asian patients with cancer-associated VTE, use of a NOAC was associated with a similar risk for recurrent VTE or major bleeding compared with use of the LMWH enoxaparin.
33533929	8	14	theme	inverse	1200:1206	arg1	probability					1208:1218	Stabilized inverse probability	1189:1218	Stabilized inverse probability of treatment weighting	1189:1241	Stabilized inverse probability of treatment weighting was used to balance baseline covariates.
33533929	3	15	theme	electronic	762:771	arg1	records					781:787	electronic medical records	762:787	a multi-institutional electronic medical records database in Taiwan	740:806	Design, Setting, and Participants This cohort study was conducted using data from the Chang Gung Research Database, a multi-institutional electronic medical records database in Taiwan.
33533929	1	16	theme	K	248:248	arg1	NOACs					282:286	NOACs	282:286	NOACs	282:286	Importance It is unclear whether the clinical benefits associated with non-vitamin K antagonist oral anticoagulants (NOACs) are similar to those associated with low-molecular-weight heparins (LMWHs) in Asian individuals with cancer and acute venous thromboembolism (VTE).
33533929	1	16	theme	K	248:248	arg1	anticoagulants					266:279	non-vitamin K antagonist oral anticoagulants	236:279	non-vitamin K antagonist oral anticoagulants (NOACs)	236:287	Importance It is unclear whether the clinical benefits associated with non-vitamin K antagonist oral anticoagulants (NOACs) are similar to those associated with low-molecular-weight heparins (LMWHs) in Asian individuals with cancer and acute venous thromboembolism (VTE).
33533929	14	17	dep	HR	2313:2314	arg1	CI					2327:2328	95% CI	2323:2328	95% CI	2323:2328	However, taking a NOAC was associated with a significantly lower risk of gastrointestinal bleeding compared with receiving enoxaparin (10 patients [1.9%] vs 41 patients [7.1%]; HR, 0.29; 95% CI, 0.15-0.59; P < .001).
33533929	2	18	theme	LMWH	559:562	arg1	enoxaparin					564:573	the LMWH enoxaparin	555:573	the LMWH enoxaparin	555:573	Objective To compare the risk of recurrent thromboembolic events and bleeding associated with use of a NOAC vs use of the LMWH enoxaparin in Asian individuals with cancer-associated VTE.
33533929	13	19	dep	had	1977:1979	arg1	HR					2071:2072	HR	2071:2072	HR	2071:2072	The groups had similar risk of VTE recurrence (HR, 0.62; 95% CI, 0.39-1.01; P = .05) and major bleeding (HR, 0.80; 95% CI, 0.52-1.24; P = .32) at 12 months of follow-up.
33533929	7	20	theme	recurrent	1156:1164	arg1	VTE					1166:1168	composite recurrent VTE	1146:1168	composite recurrent VTE	1146:1168	Main Outcomes and Measures The primary outcomes were composite recurrent VTE or major bleeding.
33533929	1	21	theme	oral	261:264	arg1	NOACs					282:286	NOACs	282:286	NOACs	282:286	Importance It is unclear whether the clinical benefits associated with non-vitamin K antagonist oral anticoagulants (NOACs) are similar to those associated with low-molecular-weight heparins (LMWHs) in Asian individuals with cancer and acute venous thromboembolism (VTE).
33533929	1	21	theme	oral	261:264	arg1	anticoagulants					266:279	non-vitamin K antagonist oral anticoagulants	236:279	non-vitamin K antagonist oral anticoagulants (NOACs)	236:287	Importance It is unclear whether the clinical benefits associated with non-vitamin K antagonist oral anticoagulants (NOACs) are similar to those associated with low-molecular-weight heparins (LMWHs) in Asian individuals with cancer and acute venous thromboembolism (VTE).
33533929	8	22	theme	weighting	1233:1241	arg1	probability					1208:1218	Stabilized inverse probability	1189:1218	Stabilized inverse probability of treatment weighting	1189:1241	Stabilized inverse probability of treatment weighting was used to balance baseline covariates.
33533929	2	23	theme	cancer-associated	601:617	arg1	VTE					619:621	cancer-associated VTE	601:621	cancer-associated VTE	601:621	Objective To compare the risk of recurrent thromboembolic events and bleeding associated with use of a NOAC vs use of the LMWH enoxaparin in Asian individuals with cancer-associated VTE.
33533929	12	24	theme	hazard	1912:1917	arg1	group					1896:1900	the enoxaparin group	1881:1900	the enoxaparin group (weighted hazard ratio [HR]	1881:1928	Composite recurrent VTE or major bleeding occurred in 75 patients (14.1%) in the NOAC group and 101 patients (17.4%) in the enoxaparin group (weighted hazard ratio [HR], 0.77; 95% CI, 0.56-1.07; P = .11).
33533929	12	24	theme	hazard	1912:1917	arg1	[HR					1925:1927	weighted hazard ratio [HR	1903:1927	weighted hazard ratio [HR	1903:1927	Composite recurrent VTE or major bleeding occurred in 75 patients (14.1%) in the NOAC group and 101 patients (17.4%) in the enoxaparin group (weighted hazard ratio [HR], 0.77; 95% CI, 0.56-1.07; P = .11).
33533929	12	25	dep	occurred	1803:1810	arg1	0.77					1931:1934	0.77	1931:1934	0.77	1931:1934	Composite recurrent VTE or major bleeding occurred in 75 patients (14.1%) in the NOAC group and 101 patients (17.4%) in the enoxaparin group (weighted hazard ratio [HR], 0.77; 95% CI, 0.56-1.07; P = .11).
33533929	2	26	from	NOAC	540:543	arg1	individuals					584:594	Asian individuals	578:594	Asian individuals with cancer-associated VTE	578:621	Objective To compare the risk of recurrent thromboembolic events and bleeding associated with use of a NOAC vs use of the LMWH enoxaparin in Asian individuals with cancer-associated VTE.
33533929	11	27	theme	529	1670:1672	arg1	patients					1674:1681	529 patients	1670:1681	529 patients (47.7%)	1670:1689	Results Among 1109 patients with cancer and newly diagnosed VTE, 578 (52.1%) were women and the mean (SD) age at index date was 66.0 (13.0) years; 529 patients (47.7%) received NOACs and 580 patients (52.3%) received the LMWH enoxaparin.
33533929	11	27	theme	529	1670:1672	arg1	%					1688:1688	47.7%	1684:1688	47.7%	1684:1688	Results Among 1109 patients with cancer and newly diagnosed VTE, 578 (52.1%) were women and the mean (SD) age at index date was 66.0 (13.0) years; 529 patients (47.7%) received NOACs and 580 patients (52.3%) received the LMWH enoxaparin.
33533929	15	28	theme	recurrent	2434:2442	arg1	VTE					2444:2446	recurrent VTE	2434:2446	recurrent VTE	2434:2446	Findings for both primary outcomes were consistent with competing risk analyses (recurrent VTE: HR, 0.68; 95% CI, 0.45-1.01; P = .05; major bleeding: HR, 0.77; 95% CI, 0.51-1.16; P = .21).
33533929	14	29	theme	[1.9	2283:2286	arg1	%					2287:2287	%	2287:2287	%	2287:2287	However, taking a NOAC was associated with a significantly lower risk of gastrointestinal bleeding compared with receiving enoxaparin (10 patients [1.9%] vs 41 patients [7.1%]; HR, 0.29; 95% CI, 0.15-0.59; P < .001).
33533929	12	30	theme	enoxaparin	1885:1894	arg1	group					1896:1900	the enoxaparin group	1881:1900	the enoxaparin group (weighted hazard ratio [HR]	1881:1928	Composite recurrent VTE or major bleeding occurred in 75 patients (14.1%) in the NOAC group and 101 patients (17.4%) in the enoxaparin group (weighted hazard ratio [HR], 0.77; 95% CI, 0.56-1.07; P = .11).
33533929	12	30	theme	enoxaparin	1885:1894	arg1	[HR					1925:1927	weighted hazard ratio [HR	1903:1927	weighted hazard ratio [HR	1903:1927	Composite recurrent VTE or major bleeding occurred in 75 patients (14.1%) in the NOAC group and 101 patients (17.4%) in the enoxaparin group (weighted hazard ratio [HR], 0.77; 95% CI, 0.56-1.07; P = .11).
33533929	0	31	theme	Low-Molecular-Weight	68:87	arg1	Heparin					89:95	Low-Molecular-Weight Heparin	68:95	Low-Molecular-Weight Heparin	68:95	Comparison Between Non-vitamin K Antagonist Oral Anticoagulants and Low-Molecular-Weight Heparin in Asian Individuals With Cancer-Associated Venous Thromboembolism.
33533929	1	32	theme	Asian	367:371	arg1	individuals					373:383	Asian individuals	367:383	Asian individuals with cancer and acute venous thromboembolism (VTE)	367:434	Importance It is unclear whether the clinical benefits associated with non-vitamin K antagonist oral anticoagulants (NOACs) are similar to those associated with low-molecular-weight heparins (LMWHs) in Asian individuals with cancer and acute venous thromboembolism (VTE).
33533929	14	33	theme	%	2287:2287	arg1	patients					2274:2281	10 patients	2271:2281	10 patients [1.9%	2271:2287	However, taking a NOAC was associated with a significantly lower risk of gastrointestinal bleeding compared with receiving enoxaparin (10 patients [1.9%] vs 41 patients [7.1%]; HR, 0.29; 95% CI, 0.15-0.59; P < .001).
33533929	16	34	theme	LMWH	2780:2783	arg1	enoxaparin					2785:2794	the LMWH enoxaparin	2776:2794	the LMWH enoxaparin	2776:2794	Conclusions and Relevance This cohort study found that in real-world practice, among Asian patients with cancer-associated VTE, use of a NOAC was associated with a similar risk for recurrent VTE or major bleeding compared with use of the LMWH enoxaparin.
33533929	10	35	theme	Gray	1446:1449	arg1	model					1474:1478	a Fine and Gray subdistribution hazard model	1435:1478	a Fine and Gray subdistribution hazard model that considered death as a competing risk	1435:1520	In addition, we conducted an analysis using a Fine and Gray subdistribution hazard model that considered death as a competing risk.
33533929	4	36	with	patients	826:833	arg1	VTE					858:860	cancer-associated VTE	840:860	cancer-associated VTE	840:860	A cohort of 1109 patients with cancer-associated VTE were identified between January 1, 2012, and January 31, 2019.
33533929	0	37	from	Comparison	0:9	arg1	Individuals					106:116	Asian Individuals	100:116	Asian Individuals With Cancer-Associated Venous Thromboembolism	100:162	Comparison Between Non-vitamin K Antagonist Oral Anticoagulants and Low-Molecular-Weight Heparin in Asian Individuals With Cancer-Associated Venous Thromboembolism.
33533929	10	38	theme	Fine	1437:1440	arg1	model					1474:1478	a Fine and Gray subdistribution hazard model	1435:1478	a Fine and Gray subdistribution hazard model that considered death as a competing risk	1435:1520	In addition, we conducted an analysis using a Fine and Gray subdistribution hazard model that considered death as a competing risk.
33533929	1	39	dep	Importance	165:174	arg1	It					176:177	It	176:177	It	176:177	Importance It is unclear whether the clinical benefits associated with non-vitamin K antagonist oral anticoagulants (NOACs) are similar to those associated with low-molecular-weight heparins (LMWHs) in Asian individuals with cancer and acute venous thromboembolism (VTE).
33533929	16	40	theme	cohort	2573:2578	arg1	study					2580:2584	This cohort study	2568:2584	This cohort study	2568:2584	Conclusions and Relevance This cohort study found that in real-world practice, among Asian patients with cancer-associated VTE, use of a NOAC was associated with a similar risk for recurrent VTE or major bleeding compared with use of the LMWH enoxaparin.
33533929	9	41	theme	VTE	1315:1317	arg1	risks					1296:1300	risks	1296:1300	risks of recurrent VTE or major bleeding between groups	1296:1350	We compared risks of recurrent VTE or major bleeding between groups using Cox proportional hazards models.
33533929	16	42	theme	similar	2706:2712	arg1	risk					2714:2717	a similar risk	2704:2717	a similar risk for recurrent VTE or major bleeding	2704:2753	Conclusions and Relevance This cohort study found that in real-world practice, among Asian patients with cancer-associated VTE, use of a NOAC was associated with a similar risk for recurrent VTE or major bleeding compared with use of the LMWH enoxaparin.
33533929	11	43	from	date	1642:1645	arg1	SD					1625:1626	SD	1625:1626	SD	1625:1626	Results Among 1109 patients with cancer and newly diagnosed VTE, 578 (52.1%) were women and the mean (SD) age at index date was 66.0 (13.0) years; 529 patients (47.7%) received NOACs and 580 patients (52.3%) received the LMWH enoxaparin.
33533929	11	43	from	date	1642:1645	arg1	women					1605:1609	women	1605:1609	women	1605:1609	Results Among 1109 patients with cancer and newly diagnosed VTE, 578 (52.1%) were women and the mean (SD) age at index date was 66.0 (13.0) years; 529 patients (47.7%) received NOACs and 580 patients (52.3%) received the LMWH enoxaparin.
33533929	11	43	from	date	1642:1645	arg1	age					1629:1631	the mean (SD) age	1615:1631	the mean (SD) age at index date	1615:1645	Results Among 1109 patients with cancer and newly diagnosed VTE, 578 (52.1%) were women and the mean (SD) age at index date was 66.0 (13.0) years; 529 patients (47.7%) received NOACs and 580 patients (52.3%) received the LMWH enoxaparin.
33533929	0	44	theme	Cancer-Associated	123:139	arg1	Thromboembolism					148:162	Cancer-Associated Venous Thromboembolism	123:162	Cancer-Associated Venous Thromboembolism	123:162	Comparison Between Non-vitamin K Antagonist Oral Anticoagulants and Low-Molecular-Weight Heparin in Asian Individuals With Cancer-Associated Venous Thromboembolism.
33533929	1	45	theme	venous	407:412	arg1	VTE					431:433	VTE	431:433	VTE	431:433	Importance It is unclear whether the clinical benefits associated with non-vitamin K antagonist oral anticoagulants (NOACs) are similar to those associated with low-molecular-weight heparins (LMWHs) in Asian individuals with cancer and acute venous thromboembolism (VTE).
33533929	1	45	theme	venous	407:412	arg1	thromboembolism					414:428	acute venous thromboembolism	401:428	acute venous thromboembolism (VTE)	401:434	Importance It is unclear whether the clinical benefits associated with non-vitamin K antagonist oral anticoagulants (NOACs) are similar to those associated with low-molecular-weight heparins (LMWHs) in Asian individuals with cancer and acute venous thromboembolism (VTE).
33533929	2	46	theme	thromboembolic	480:493	arg1	events					495:500	recurrent thromboembolic events	470:500	recurrent thromboembolic events	470:500	Objective To compare the risk of recurrent thromboembolic events and bleeding associated with use of a NOAC vs use of the LMWH enoxaparin in Asian individuals with cancer-associated VTE.
33533929	10	47	theme	hazard	1467:1472	arg1	model					1474:1478	a Fine and Gray subdistribution hazard model	1435:1478	a Fine and Gray subdistribution hazard model that considered death as a competing risk	1435:1520	In addition, we conducted an analysis using a Fine and Gray subdistribution hazard model that considered death as a competing risk.
33533929	14	48	theme	taking	2145:2150	arg1	NOAC					2154:2157	a NOAC	2152:2157	taking a NOAC	2145:2157	However, taking a NOAC was associated with a significantly lower risk of gastrointestinal bleeding compared with receiving enoxaparin (10 patients [1.9%] vs 41 patients [7.1%]; HR, 0.29; 95% CI, 0.15-0.59; P < .001).
33533929	13	49	theme	recurrence	2001:2010	arg1	risk					1989:1992	similar risk	1981:1992	similar risk of VTE recurrence (HR, 0.62; 95% CI, 0.39-1.01; P = .05) and major bleeding	1981:2068	The groups had similar risk of VTE recurrence (HR, 0.62; 95% CI, 0.39-1.01; P = .05) and major bleeding (HR, 0.80; 95% CI, 0.52-1.24; P = .32) at 12 months of follow-up.
33533929	11	50	dep	Results	1523:1529	arg1	SD					1625:1626	SD	1625:1626	SD	1625:1626	Results Among 1109 patients with cancer and newly diagnosed VTE, 578 (52.1%) were women and the mean (SD) age at index date was 66.0 (13.0) years; 529 patients (47.7%) received NOACs and 580 patients (52.3%) received the LMWH enoxaparin.
33533929	11	50	dep	Results	1523:1529	arg1	women					1605:1609	women	1605:1609	women	1605:1609	Results Among 1109 patients with cancer and newly diagnosed VTE, 578 (52.1%) were women and the mean (SD) age at index date was 66.0 (13.0) years; 529 patients (47.7%) received NOACs and 580 patients (52.3%) received the LMWH enoxaparin.
33533929	11	50	dep	Results	1523:1529	arg1	age					1629:1631	the mean (SD) age	1615:1631	the mean (SD) age at index date	1615:1645	Results Among 1109 patients with cancer and newly diagnosed VTE, 578 (52.1%) were women and the mean (SD) age at index date was 66.0 (13.0) years; 529 patients (47.7%) received NOACs and 580 patients (52.3%) received the LMWH enoxaparin.
33533929	13	51	dep	HR	2071:2072	arg1	P = .32					2100:2106	P = .32	2100:2106	P = .32	2100:2106	The groups had similar risk of VTE recurrence (HR, 0.62; 95% CI, 0.39-1.01; P = .05) and major bleeding (HR, 0.80; 95% CI, 0.52-1.24; P = .32) at 12 months of follow-up.
33533929	13	51	dep	HR	2071:2072	arg1	CI					2085:2086	95% CI	2081:2086	95% CI	2081:2086	The groups had similar risk of VTE recurrence (HR, 0.62; 95% CI, 0.39-1.01; P = .05) and major bleeding (HR, 0.80; 95% CI, 0.52-1.24; P = .32) at 12 months of follow-up.
33533929	0	52	theme	Non-vitamin	19:29	arg1	Anticoagulants					49:62	Non-vitamin K Antagonist Oral Anticoagulants	19:62	Non-vitamin K Antagonist Oral Anticoagulants	19:62	Comparison Between Non-vitamin K Antagonist Oral Anticoagulants and Low-Molecular-Weight Heparin in Asian Individuals With Cancer-Associated Venous Thromboembolism.
33533929	1	53	theme	low-molecular-weight	326:345	arg1	LMWHs					357:361	LMWHs	357:361	LMWHs	357:361	Importance It is unclear whether the clinical benefits associated with non-vitamin K antagonist oral anticoagulants (NOACs) are similar to those associated with low-molecular-weight heparins (LMWHs) in Asian individuals with cancer and acute venous thromboembolism (VTE).
33533929	1	53	theme	low-molecular-weight	326:345	arg1	heparins					347:354	low-molecular-weight heparins	326:354	low-molecular-weight heparins (LMWHs) in Asian individuals with cancer and acute venous thromboembolism (VTE)	326:434	Importance It is unclear whether the clinical benefits associated with non-vitamin K antagonist oral anticoagulants (NOACs) are similar to those associated with low-molecular-weight heparins (LMWHs) in Asian individuals with cancer and acute venous thromboembolism (VTE).
33533929	2	54	dep	NOAC	540:543	arg1	a					538:538	a	538:538	a	538:538	Objective To compare the risk of recurrent thromboembolic events and bleeding associated with use of a NOAC vs use of the LMWH enoxaparin in Asian individuals with cancer-associated VTE.
33533929	4	55	theme	cancer-associated	840:856	arg1	VTE					858:860	cancer-associated VTE	840:860	cancer-associated VTE	840:860	A cohort of 1109 patients with cancer-associated VTE were identified between January 1, 2012, and January 31, 2019.
33533929	7	56	theme	composite	1146:1154	arg1	VTE					1166:1168	composite recurrent VTE	1146:1168	composite recurrent VTE	1146:1168	Main Outcomes and Measures The primary outcomes were composite recurrent VTE or major bleeding.
33533929	9	57	theme	proportional	1362:1373	arg1	models					1383:1388	Cox proportional hazards models	1358:1388	Cox proportional hazards models	1358:1388	We compared risks of recurrent VTE or major bleeding between groups using Cox proportional hazards models.
33533929	0	58	theme	Antagonist	33:42	arg1	Anticoagulants					49:62	Non-vitamin K Antagonist Oral Anticoagulants	19:62	Non-vitamin K Antagonist Oral Anticoagulants	19:62	Comparison Between Non-vitamin K Antagonist Oral Anticoagulants and Low-Molecular-Weight Heparin in Asian Individuals With Cancer-Associated Venous Thromboembolism.
33533929	12	59	dep	0.77	1931:1934	arg1	P = .11					1956:1962	P = .11	1956:1962	P = .11	1956:1962	Composite recurrent VTE or major bleeding occurred in 75 patients (14.1%) in the NOAC group and 101 patients (17.4%) in the enoxaparin group (weighted hazard ratio [HR], 0.77; 95% CI, 0.56-1.07; P = .11).
33533929	12	59	dep	0.77	1931:1934	arg1	0.56-1.07					1945:1953	0.56-1.07	1945:1953	0.56-1.07	1945:1953	Composite recurrent VTE or major bleeding occurred in 75 patients (14.1%) in the NOAC group and 101 patients (17.4%) in the enoxaparin group (weighted hazard ratio [HR], 0.77; 95% CI, 0.56-1.07; P = .11).
33533929	12	59	dep	0.77	1931:1934	arg1	CI					1941:1942	95% CI	1937:1942	95% CI	1937:1942	Composite recurrent VTE or major bleeding occurred in 75 patients (14.1%) in the NOAC group and 101 patients (17.4%) in the enoxaparin group (weighted hazard ratio [HR], 0.77; 95% CI, 0.56-1.07; P = .11).
33533929	13	60	contain	had	1977:1979	arg1	groups					1970:1975	The groups	1966:1975	The groups	1966:1975	The groups had similar risk of VTE recurrence (HR, 0.62; 95% CI, 0.39-1.01; P = .05) and major bleeding (HR, 0.80; 95% CI, 0.52-1.24; P = .32) at 12 months of follow-up.
33533929	13	60	contain	had	1977:1979	arg2	risk					1989:1992	similar risk	1981:1992	similar risk of VTE recurrence (HR, 0.62; 95% CI, 0.39-1.01; P = .05) and major bleeding	1981:2068	The groups had similar risk of VTE recurrence (HR, 0.62; 95% CI, 0.39-1.01; P = .05) and major bleeding (HR, 0.80; 95% CI, 0.52-1.24; P = .32) at 12 months of follow-up.
33533929	16	61	dep	Conclusions	2542:2552	arg1	found					2586:2590	found	2586:2590	found that in real-world practice, among Asian patients with cancer-associated VTE, use of a NOAC was associated with a similar risk for recurrent VTE or major bleeding compared with use of the LMWH enoxaparin	2586:2794	Conclusions and Relevance This cohort study found that in real-world practice, among Asian patients with cancer-associated VTE, use of a NOAC was associated with a similar risk for recurrent VTE or major bleeding compared with use of the LMWH enoxaparin.
33533929	18	62	theme	prospective	2909:2919	arg1	studies					2921:2927	Further prospective studies	2901:2927	Further prospective studies	2901:2927	Further prospective studies are needed to confirm these findings.
33533929	13	63	theme	similar	1981:1987	arg1	risk					1989:1992	similar risk	1981:1992	similar risk of VTE recurrence (HR, 0.62; 95% CI, 0.39-1.01; P = .05) and major bleeding	1981:2068	The groups had similar risk of VTE recurrence (HR, 0.62; 95% CI, 0.39-1.01; P = .05) and major bleeding (HR, 0.80; 95% CI, 0.52-1.24; P = .32) at 12 months of follow-up.
33533929	11	64	theme	index	1636:1640	arg1	date					1642:1645	index date	1636:1645	index date	1636:1645	Results Among 1109 patients with cancer and newly diagnosed VTE, 578 (52.1%) were women and the mean (SD) age at index date was 66.0 (13.0) years; 529 patients (47.7%) received NOACs and 580 patients (52.3%) received the LMWH enoxaparin.
33533929	12	65	theme	Composite	1761:1769	arg1	VTE					1781:1783	Composite recurrent VTE	1761:1783	Composite recurrent VTE	1761:1783	Composite recurrent VTE or major bleeding occurred in 75 patients (14.1%) in the NOAC group and 101 patients (17.4%) in the enoxaparin group (weighted hazard ratio [HR], 0.77; 95% CI, 0.56-1.07; P = .11).
33533929	16	66	theme	enoxaparin	2785:2794	arg1	use					2769:2771	use	2769:2771	use of the LMWH enoxaparin	2769:2794	Conclusions and Relevance This cohort study found that in real-world practice, among Asian patients with cancer-associated VTE, use of a NOAC was associated with a similar risk for recurrent VTE or major bleeding compared with use of the LMWH enoxaparin.
33533929	7	67	theme	Main	1093:1096	arg1	Outcomes					1098:1105	Main Outcomes	1093:1105	Main Outcomes	1093:1105	Main Outcomes and Measures The primary outcomes were composite recurrent VTE or major bleeding.
33533929	16	68	theme	cancer-associated	2647:2663	arg1	VTE					2665:2667	cancer-associated VTE	2647:2667	cancer-associated VTE	2647:2667	Conclusions and Relevance This cohort study found that in real-world practice, among Asian patients with cancer-associated VTE, use of a NOAC was associated with a similar risk for recurrent VTE or major bleeding compared with use of the LMWH enoxaparin.
33533929	14	69	theme	95	2323:2324	arg1	%					2325:2325	%	2325:2325	%	2325:2325	However, taking a NOAC was associated with a significantly lower risk of gastrointestinal bleeding compared with receiving enoxaparin (10 patients [1.9%] vs 41 patients [7.1%]; HR, 0.29; 95% CI, 0.15-0.59; P < .001).
33533929	4	70	theme	patients	826:833	arg1	cohort					811:816	A cohort	809:816	A cohort of 1109 patients with cancer-associated VTE	809:860	A cohort of 1109 patients with cancer-associated VTE were identified between January 1, 2012, and January 31, 2019.
33533929	13	71	theme	%	2083:2083	arg1	CI					2085:2086	95% CI	2081:2086	95% CI	2081:2086	The groups had similar risk of VTE recurrence (HR, 0.62; 95% CI, 0.39-1.01; P = .05) and major bleeding (HR, 0.80; 95% CI, 0.52-1.24; P = .32) at 12 months of follow-up.
33533929	7	72	dep	Outcomes	1098:1105	arg1	outcomes					1132:1139	The primary outcomes	1120:1139	The primary outcomes	1120:1139	Main Outcomes and Measures The primary outcomes were composite recurrent VTE or major bleeding.
33533929	14	73	dep	enoxaparin	2259:2268	arg1	patients					2274:2281	10 patients	2271:2281	10 patients [1.9%	2271:2287	However, taking a NOAC was associated with a significantly lower risk of gastrointestinal bleeding compared with receiving enoxaparin (10 patients [1.9%] vs 41 patients [7.1%]; HR, 0.29; 95% CI, 0.15-0.59; P < .001).
33533929	3	74	theme	Research	721:728	arg1	database					789:796	a multi-institutional electronic medical records database	740:796	a multi-institutional electronic medical records database in Taiwan	740:806	Design, Setting, and Participants This cohort study was conducted using data from the Chang Gung Research Database, a multi-institutional electronic medical records database in Taiwan.
33533929	3	74	theme	Research	721:728	arg1	Database					730:737	the Chang Gung Research Database	706:737	the Chang Gung Research Database	706:737	Design, Setting, and Participants This cohort study was conducted using data from the Chang Gung Research Database, a multi-institutional electronic medical records database in Taiwan.
33533929	15	75	theme	risk	2419:2422	arg1	analyses					2424:2431	competing risk analyses	2409:2431	competing risk analyses	2409:2431	Findings for both primary outcomes were consistent with competing risk analyses (recurrent VTE: HR, 0.68; 95% CI, 0.45-1.01; P = .05; major bleeding: HR, 0.77; 95% CI, 0.51-1.16; P = .21).
33533929	6	76	theme	LMWH	1076:1079	arg1	enoxaparin					1081:1090	the LMWH enoxaparin	1072:1090	the LMWH enoxaparin	1072:1090	Exposures Receiving a NOAC (including rivaroxaban, apixaban, edoxaban, or dabigatran) or the LMWH enoxaparin.
33533929	16	77	theme	NOAC	2679:2682	arg1	use					2670:2672	use	2670:2672	use of a NOAC	2670:2682	Conclusions and Relevance This cohort study found that in real-world practice, among Asian patients with cancer-associated VTE, use of a NOAC was associated with a similar risk for recurrent VTE or major bleeding compared with use of the LMWH enoxaparin.
33533929	15	78	dep	VTE	2444:2446	arg1	CI					2463:2464	95% CI	2459:2464	95% CI	2459:2464	Findings for both primary outcomes were consistent with competing risk analyses (recurrent VTE: HR, 0.68; 95% CI, 0.45-1.01; P = .05; major bleeding: HR, 0.77; 95% CI, 0.51-1.16; P = .21).
33533929	15	78	dep	VTE	2444:2446	arg1	HR					2503:2504	HR	2503:2504	HR	2503:2504	Findings for both primary outcomes were consistent with competing risk analyses (recurrent VTE: HR, 0.68; 95% CI, 0.45-1.01; P = .05; major bleeding: HR, 0.77; 95% CI, 0.51-1.16; P = .21).
33533929	15	78	dep	VTE	2444:2446	arg1	0.68					2453:2456	0.68	2453:2456	0.68	2453:2456	Findings for both primary outcomes were consistent with competing risk analyses (recurrent VTE: HR, 0.68; 95% CI, 0.45-1.01; P = .05; major bleeding: HR, 0.77; 95% CI, 0.51-1.16; P = .21).
33533929	15	78	dep	VTE	2444:2446	arg1	0.45-1.01					2467:2475	0.45-1.01	2467:2475	0.45-1.01; P = .05; major bleeding	2467:2500	Findings for both primary outcomes were consistent with competing risk analyses (recurrent VTE: HR, 0.68; 95% CI, 0.45-1.01; P = .05; major bleeding: HR, 0.77; 95% CI, 0.51-1.16; P = .21).
33533929	15	78	dep	VTE	2444:2446	arg1	HR					2449:2450	HR	2449:2450	HR	2449:2450	Findings for both primary outcomes were consistent with competing risk analyses (recurrent VTE: HR, 0.68; 95% CI, 0.45-1.01; P = .05; major bleeding: HR, 0.77; 95% CI, 0.51-1.16; P = .21).
33533929	3	79	theme	multi-institutional	742:760	arg1	database					789:796	a multi-institutional electronic medical records database	740:796	a multi-institutional electronic medical records database in Taiwan	740:806	Design, Setting, and Participants This cohort study was conducted using data from the Chang Gung Research Database, a multi-institutional electronic medical records database in Taiwan.
33533929	3	79	theme	multi-institutional	742:760	arg1	Database					730:737	the Chang Gung Research Database	706:737	the Chang Gung Research Database	706:737	Design, Setting, and Participants This cohort study was conducted using data from the Chang Gung Research Database, a multi-institutional electronic medical records database in Taiwan.
33533929	1	80	theme	clinical	202:209	arg1	similar					293:299	similar	293:299	similar	293:299	Importance It is unclear whether the clinical benefits associated with non-vitamin K antagonist oral anticoagulants (NOACs) are similar to those associated with low-molecular-weight heparins (LMWHs) in Asian individuals with cancer and acute venous thromboembolism (VTE).
33533929	1	80	theme	clinical	202:209	arg1	benefits					211:218	the clinical benefits	198:218	the clinical benefits associated with non-vitamin K antagonist oral anticoagulants (NOACs)	198:287	Importance It is unclear whether the clinical benefits associated with non-vitamin K antagonist oral anticoagulants (NOACs) are similar to those associated with low-molecular-weight heparins (LMWHs) in Asian individuals with cancer and acute venous thromboembolism (VTE).
33533929	18	81	theme	Further	2901:2907	arg1	studies					2921:2927	Further prospective studies	2901:2927	Further prospective studies	2901:2927	Further prospective studies are needed to confirm these findings.
33533929	16	82	with	patients	2633:2640	arg1	VTE					2665:2667	cancer-associated VTE	2647:2667	cancer-associated VTE	2647:2667	Conclusions and Relevance This cohort study found that in real-world practice, among Asian patients with cancer-associated VTE, use of a NOAC was associated with a similar risk for recurrent VTE or major bleeding compared with use of the LMWH enoxaparin.
33533929	3	83	from	Database	730:737	arg1	data					696:699	data	696:699	data from the Chang Gung Research Database, a multi-institutional electronic medical records database in Taiwan	696:806	Design, Setting, and Participants This cohort study was conducted using data from the Chang Gung Research Database, a multi-institutional electronic medical records database in Taiwan.
33533929	13	84	theme	bleeding	2061:2068	arg1	risk					1989:1992	similar risk	1981:1992	similar risk of VTE recurrence (HR, 0.62; 95% CI, 0.39-1.01; P = .05) and major bleeding	1981:2068	The groups had similar risk of VTE recurrence (HR, 0.62; 95% CI, 0.39-1.01; P = .05) and major bleeding (HR, 0.80; 95% CI, 0.52-1.24; P = .32) at 12 months of follow-up.
33533929	15	85	theme	primary	2371:2377	arg1	outcomes					2379:2386	both primary outcomes	2366:2386	both primary outcomes	2366:2386	Findings for both primary outcomes were consistent with competing risk analyses (recurrent VTE: HR, 0.68; 95% CI, 0.45-1.01; P = .05; major bleeding: HR, 0.77; 95% CI, 0.51-1.16; P = .21).
33533929	11	86	theme	diagnosed	1573:1581	arg1	VTE					1583:1585	newly diagnosed VTE	1567:1585	newly diagnosed VTE	1567:1585	Results Among 1109 patients with cancer and newly diagnosed VTE, 578 (52.1%) were women and the mean (SD) age at index date was 66.0 (13.0) years; 529 patients (47.7%) received NOACs and 580 patients (52.3%) received the LMWH enoxaparin.
33533929	3	87	dep	Design	624:629	arg1	study					670:674	This cohort study	658:674	This cohort study	658:674	Design, Setting, and Participants This cohort study was conducted using data from the Chang Gung Research Database, a multi-institutional electronic medical records database in Taiwan.
33533929	0	88	with	Individuals	106:116	arg1	Thromboembolism					148:162	Cancer-Associated Venous Thromboembolism	123:162	Cancer-Associated Venous Thromboembolism	123:162	Comparison Between Non-vitamin K Antagonist Oral Anticoagulants and Low-Molecular-Weight Heparin in Asian Individuals With Cancer-Associated Venous Thromboembolism.
33533929	1	89	theme	non-vitamin	236:246	arg1	NOACs					282:286	NOACs	282:286	NOACs	282:286	Importance It is unclear whether the clinical benefits associated with non-vitamin K antagonist oral anticoagulants (NOACs) are similar to those associated with low-molecular-weight heparins (LMWHs) in Asian individuals with cancer and acute venous thromboembolism (VTE).
33533929	1	89	theme	non-vitamin	236:246	arg1	anticoagulants					266:279	non-vitamin K antagonist oral anticoagulants	236:279	non-vitamin K antagonist oral anticoagulants (NOACs)	236:287	Importance It is unclear whether the clinical benefits associated with non-vitamin K antagonist oral anticoagulants (NOACs) are similar to those associated with low-molecular-weight heparins (LMWHs) in Asian individuals with cancer and acute venous thromboembolism (VTE).
33533929	8	90	theme	Stabilized	1189:1198	arg1	probability					1208:1218	Stabilized inverse probability	1189:1218	Stabilized inverse probability of treatment weighting	1189:1241	Stabilized inverse probability of treatment weighting was used to balance baseline covariates.
33533929	2	91	theme	enoxaparin	564:573	arg1	NOAC					540:543	NOAC	540:543	NOAC	540:543	Objective To compare the risk of recurrent thromboembolic events and bleeding associated with use of a NOAC vs use of the LMWH enoxaparin in Asian individuals with cancer-associated VTE.
33533929	2	92	from	use	531:533	arg1	individuals					584:594	Asian individuals	578:594	Asian individuals with cancer-associated VTE	578:621	Objective To compare the risk of recurrent thromboembolic events and bleeding associated with use of a NOAC vs use of the LMWH enoxaparin in Asian individuals with cancer-associated VTE.
33533929	15	93	theme	%	2461:2461	arg1	CI					2463:2464	95% CI	2459:2464	95% CI	2459:2464	Findings for both primary outcomes were consistent with competing risk analyses (recurrent VTE: HR, 0.68; 95% CI, 0.45-1.01; P = .05; major bleeding: HR, 0.77; 95% CI, 0.51-1.16; P = .21).
33533929	8	94	theme	baseline	1263:1270	arg1	covariates					1272:1281	balance baseline covariates	1255:1281	balance baseline covariates	1255:1281	Stabilized inverse probability of treatment weighting was used to balance baseline covariates.
33533929	3	95	theme	medical	773:779	arg1	records					781:787	electronic medical records	762:787	a multi-institutional electronic medical records database in Taiwan	740:806	Design, Setting, and Participants This cohort study was conducted using data from the Chang Gung Research Database, a multi-institutional electronic medical records database in Taiwan.
33533929	1	96	theme	antagonist	250:259	arg1	NOACs					282:286	NOACs	282:286	NOACs	282:286	Importance It is unclear whether the clinical benefits associated with non-vitamin K antagonist oral anticoagulants (NOACs) are similar to those associated with low-molecular-weight heparins (LMWHs) in Asian individuals with cancer and acute venous thromboembolism (VTE).
33533929	1	96	theme	antagonist	250:259	arg1	anticoagulants					266:279	non-vitamin K antagonist oral anticoagulants	236:279	non-vitamin K antagonist oral anticoagulants (NOACs)	236:287	Importance It is unclear whether the clinical benefits associated with non-vitamin K antagonist oral anticoagulants (NOACs) are similar to those associated with low-molecular-weight heparins (LMWHs) in Asian individuals with cancer and acute venous thromboembolism (VTE).
33533929	15	97	dep	0.45-1.01	2467:2475	arg1	P = .05					2478:2484	P = .05	2478:2484	P = .05	2478:2484	Findings for both primary outcomes were consistent with competing risk analyses (recurrent VTE: HR, 0.68; 95% CI, 0.45-1.01; P = .05; major bleeding: HR, 0.77; 95% CI, 0.51-1.16; P = .21).
33533929	15	97	dep	0.45-1.01	2467:2475	arg1	bleeding					2493:2500	bleeding	2493:2500	bleeding	2493:2500	Findings for both primary outcomes were consistent with competing risk analyses (recurrent VTE: HR, 0.68; 95% CI, 0.45-1.01; P = .05; major bleeding: HR, 0.77; 95% CI, 0.51-1.16; P = .21).
33533929	8	98	theme	treatment	1223:1231	arg1	weighting					1233:1241	treatment weighting	1223:1241	treatment weighting	1223:1241	Stabilized inverse probability of treatment weighting was used to balance baseline covariates.
33533929	3	99	theme	cohort	663:668	arg1	study					670:674	This cohort study	658:674	This cohort study	658:674	Design, Setting, and Participants This cohort study was conducted using data from the Chang Gung Research Database, a multi-institutional electronic medical records database in Taiwan.
33533929	15	100	theme	0.77	2507:2510	arg1	HR					2503:2504	HR	2503:2504	HR	2503:2504	Findings for both primary outcomes were consistent with competing risk analyses (recurrent VTE: HR, 0.68; 95% CI, 0.45-1.01; P = .05; major bleeding: HR, 0.77; 95% CI, 0.51-1.16; P = .21).
33533929	12	101	theme	weighted	1903:1910	arg1	group					1896:1900	the enoxaparin group	1881:1900	the enoxaparin group (weighted hazard ratio [HR]	1881:1928	Composite recurrent VTE or major bleeding occurred in 75 patients (14.1%) in the NOAC group and 101 patients (17.4%) in the enoxaparin group (weighted hazard ratio [HR], 0.77; 95% CI, 0.56-1.07; P = .11).
33533929	12	101	theme	weighted	1903:1910	arg1	[HR					1925:1927	weighted hazard ratio [HR	1903:1927	weighted hazard ratio [HR	1903:1927	Composite recurrent VTE or major bleeding occurred in 75 patients (14.1%) in the NOAC group and 101 patients (17.4%) in the enoxaparin group (weighted hazard ratio [HR], 0.77; 95% CI, 0.56-1.07; P = .11).
33533929	17	102	theme	NOAC	2819:2822	arg1	use					2810:2812	use	2810:2812	use of a NOAC	2810:2822	Nonetheless, use of a NOAC was associated with a significantly lower rate of gastrointestinal bleeding.
33533929	12	103	from	patients	1818:1825	arg1	group					1847:1851	the NOAC group	1838:1851	the NOAC group	1838:1851	Composite recurrent VTE or major bleeding occurred in 75 patients (14.1%) in the NOAC group and 101 patients (17.4%) in the enoxaparin group (weighted hazard ratio [HR], 0.77; 95% CI, 0.56-1.07; P = .11).
33533929	2	104	theme	Asian	578:582	arg1	individuals					584:594	Asian individuals	578:594	Asian individuals with cancer-associated VTE	578:621	Objective To compare the risk of recurrent thromboembolic events and bleeding associated with use of a NOAC vs use of the LMWH enoxaparin in Asian individuals with cancer-associated VTE.
33533929	12	105	theme	ratio	1919:1923	arg1	group					1896:1900	the enoxaparin group	1881:1900	the enoxaparin group (weighted hazard ratio [HR]	1881:1928	Composite recurrent VTE or major bleeding occurred in 75 patients (14.1%) in the NOAC group and 101 patients (17.4%) in the enoxaparin group (weighted hazard ratio [HR], 0.77; 95% CI, 0.56-1.07; P = .11).
33533929	12	105	theme	ratio	1919:1923	arg1	[HR					1925:1927	weighted hazard ratio [HR	1903:1927	weighted hazard ratio [HR	1903:1927	Composite recurrent VTE or major bleeding occurred in 75 patients (14.1%) in the NOAC group and 101 patients (17.4%) in the enoxaparin group (weighted hazard ratio [HR], 0.77; 95% CI, 0.56-1.07; P = .11).
33533929	13	106	dep	HR	2013:2014	arg1	P = .05					2042:2048	P = .05	2042:2048	P = .05	2042:2048	The groups had similar risk of VTE recurrence (HR, 0.62; 95% CI, 0.39-1.01; P = .05) and major bleeding (HR, 0.80; 95% CI, 0.52-1.24; P = .32) at 12 months of follow-up.
33533929	12	107	theme	NOAC	1842:1845	arg1	group					1847:1851	the NOAC group	1838:1851	the NOAC group	1838:1851	Composite recurrent VTE or major bleeding occurred in 75 patients (14.1%) in the NOAC group and 101 patients (17.4%) in the enoxaparin group (weighted hazard ratio [HR], 0.77; 95% CI, 0.56-1.07; P = .11).
33533929	0	108	theme	Asian	100:104	arg1	Individuals					106:116	Asian Individuals	100:116	Asian Individuals With Cancer-Associated Venous Thromboembolism	100:162	Comparison Between Non-vitamin K Antagonist Oral Anticoagulants and Low-Molecular-Weight Heparin in Asian Individuals With Cancer-Associated Venous Thromboembolism.
33533929	13	109	dep	recurrence	2001:2010	arg1	0.39-1.01					2031:2039	0.39-1.01	2031:2039	0.39-1.01	2031:2039	The groups had similar risk of VTE recurrence (HR, 0.62; 95% CI, 0.39-1.01; P = .05) and major bleeding (HR, 0.80; 95% CI, 0.52-1.24; P = .32) at 12 months of follow-up.
33533929	13	109	dep	recurrence	2001:2010	arg1	%					2025:2025	0.62; 95%	2017:2025	0.62; 95% CI	2017:2028	The groups had similar risk of VTE recurrence (HR, 0.62; 95% CI, 0.39-1.01; P = .05) and major bleeding (HR, 0.80; 95% CI, 0.52-1.24; P = .32) at 12 months of follow-up.
33533929	13	109	dep	recurrence	2001:2010	arg1	HR					2013:2014	HR	2013:2014	HR	2013:2014	The groups had similar risk of VTE recurrence (HR, 0.62; 95% CI, 0.39-1.01; P = .05) and major bleeding (HR, 0.80; 95% CI, 0.52-1.24; P = .32) at 12 months of follow-up.
33533929	17	110	theme	lower	2860:2864	arg1	rate					2866:2869	a significantly lower rate	2844:2869	a significantly lower rate of gastrointestinal bleeding	2844:2898	Nonetheless, use of a NOAC was associated with a significantly lower rate of gastrointestinal bleeding.
33533929	9	111	theme	recurrent	1305:1313	arg1	VTE					1315:1317	recurrent VTE	1305:1317	recurrent VTE	1305:1317	We compared risks of recurrent VTE or major bleeding between groups using Cox proportional hazards models.
33533929	1	112	with	individuals	373:383	arg1	cancer					390:395	cancer	390:395	cancer	390:395	Importance It is unclear whether the clinical benefits associated with non-vitamin K antagonist oral anticoagulants (NOACs) are similar to those associated with low-molecular-weight heparins (LMWHs) in Asian individuals with cancer and acute venous thromboembolism (VTE).
33533929	1	112	with	individuals	373:383	arg1	VTE					431:433	VTE	431:433	VTE	431:433	Importance It is unclear whether the clinical benefits associated with non-vitamin K antagonist oral anticoagulants (NOACs) are similar to those associated with low-molecular-weight heparins (LMWHs) in Asian individuals with cancer and acute venous thromboembolism (VTE).
33533929	1	112	with	individuals	373:383	arg1	thromboembolism					414:428	acute venous thromboembolism	401:428	acute venous thromboembolism (VTE)	401:434	Importance It is unclear whether the clinical benefits associated with non-vitamin K antagonist oral anticoagulants (NOACs) are similar to those associated with low-molecular-weight heparins (LMWHs) in Asian individuals with cancer and acute venous thromboembolism (VTE).
33533929	2	113	theme	use	548:550	arg1	use					531:533	use	531:533	use of a NOAC vs use of the LMWH enoxaparin in Asian individuals with cancer-associated VTE	531:621	Objective To compare the risk of recurrent thromboembolic events and bleeding associated with use of a NOAC vs use of the LMWH enoxaparin in Asian individuals with cancer-associated VTE.
33533929	15	114	with	consistent	2393:2402	arg1	analyses					2424:2431	competing risk analyses	2409:2431	competing risk analyses	2409:2431	Findings for both primary outcomes were consistent with competing risk analyses (recurrent VTE: HR, 0.68; 95% CI, 0.45-1.01; P = .05; major bleeding: HR, 0.77; 95% CI, 0.51-1.16; P = .21).
33533929	0	115	theme	Venous	141:146	arg1	Thromboembolism					148:162	Cancer-Associated Venous Thromboembolism	123:162	Cancer-Associated Venous Thromboembolism	123:162	Comparison Between Non-vitamin K Antagonist Oral Anticoagulants and Low-Molecular-Weight Heparin in Asian Individuals With Cancer-Associated Venous Thromboembolism.
33533929	1	116	theme	acute	401:405	arg1	VTE					431:433	VTE	431:433	VTE	431:433	Importance It is unclear whether the clinical benefits associated with non-vitamin K antagonist oral anticoagulants (NOACs) are similar to those associated with low-molecular-weight heparins (LMWHs) in Asian individuals with cancer and acute venous thromboembolism (VTE).
33533929	1	116	theme	acute	401:405	arg1	thromboembolism					414:428	acute venous thromboembolism	401:428	acute venous thromboembolism (VTE)	401:434	Importance It is unclear whether the clinical benefits associated with non-vitamin K antagonist oral anticoagulants (NOACs) are similar to those associated with low-molecular-weight heparins (LMWHs) in Asian individuals with cancer and acute venous thromboembolism (VTE).
33533929	13	117	theme	follow-up	2125:2133	arg1	months					2115:2120	12 months	2112:2120	12 months of follow-up	2112:2133	The groups had similar risk of VTE recurrence (HR, 0.62; 95% CI, 0.39-1.01; P = .05) and major bleeding (HR, 0.80; 95% CI, 0.52-1.24; P = .32) at 12 months of follow-up.
33533929	2	118	theme	NOAC	540:543	arg1	use					531:533	use	531:533	use of a NOAC vs use of the LMWH enoxaparin in Asian individuals with cancer-associated VTE	531:621	Objective To compare the risk of recurrent thromboembolic events and bleeding associated with use of a NOAC vs use of the LMWH enoxaparin in Asian individuals with cancer-associated VTE.
33533929	9	119	theme	bleeding	1328:1335	arg1	risks					1296:1300	risks	1296:1300	risks of recurrent VTE or major bleeding between groups	1296:1350	We compared risks of recurrent VTE or major bleeding between groups using Cox proportional hazards models.
33533929	2	120	theme	recurrent	470:478	arg1	events					495:500	recurrent thromboembolic events	470:500	recurrent thromboembolic events	470:500	Objective To compare the risk of recurrent thromboembolic events and bleeding associated with use of a NOAC vs use of the LMWH enoxaparin in Asian individuals with cancer-associated VTE.
33533929	3	121	from	database	789:796	arg1	Taiwan					801:806	Taiwan	801:806	Taiwan	801:806	Design, Setting, and Participants This cohort study was conducted using data from the Chang Gung Research Database, a multi-institutional electronic medical records database in Taiwan.
33533929	13	122	dep	%	2025:2025	arg1	CI					2027:2028	CI	2027:2028	0.62; 95% CI	2017:2028	The groups had similar risk of VTE recurrence (HR, 0.62; 95% CI, 0.39-1.01; P = .05) and major bleeding (HR, 0.80; 95% CI, 0.52-1.24; P = .32) at 12 months of follow-up.
33533929	0	123	theme	K	31:31	arg1	Anticoagulants					49:62	Non-vitamin K Antagonist Oral Anticoagulants	19:62	Non-vitamin K Antagonist Oral Anticoagulants	19:62	Comparison Between Non-vitamin K Antagonist Oral Anticoagulants and Low-Molecular-Weight Heparin in Asian Individuals With Cancer-Associated Venous Thromboembolism.
33533929	16	124	theme	recurrent	2723:2731	arg1	VTE					2733:2735	recurrent VTE	2723:2735	recurrent VTE	2723:2735	Conclusions and Relevance This cohort study found that in real-world practice, among Asian patients with cancer-associated VTE, use of a NOAC was associated with a similar risk for recurrent VTE or major bleeding compared with use of the LMWH enoxaparin.
33533929	14	125	dep	patients	2296:2303	arg1	%					2309:2309	[7.1%]	2305:2310	[7.1%]	2305:2310	However, taking a NOAC was associated with a significantly lower risk of gastrointestinal bleeding compared with receiving enoxaparin (10 patients [1.9%] vs 41 patients [7.1%]; HR, 0.29; 95% CI, 0.15-0.59; P < .001).
33533929	0	126	theme	Oral	44:47	arg1	Anticoagulants					49:62	Non-vitamin K Antagonist Oral Anticoagulants	19:62	Non-vitamin K Antagonist Oral Anticoagulants	19:62	Comparison Between Non-vitamin K Antagonist Oral Anticoagulants and Low-Molecular-Weight Heparin in Asian Individuals With Cancer-Associated Venous Thromboembolism.
33533929	9	127	theme	Cox	1358:1360	arg1	models					1383:1388	Cox proportional hazards models	1358:1388	Cox proportional hazards models	1358:1388	We compared risks of recurrent VTE or major bleeding between groups using Cox proportional hazards models.
33533929	12	128	theme	recurrent	1771:1779	arg1	VTE					1781:1783	Composite recurrent VTE	1761:1783	Composite recurrent VTE	1761:1783	Composite recurrent VTE or major bleeding occurred in 75 patients (14.1%) in the NOAC group and 101 patients (17.4%) in the enoxaparin group (weighted hazard ratio [HR], 0.77; 95% CI, 0.56-1.07; P = .11).
33533929	14	129	theme	bleeding	2226:2233	arg1	risk					2201:2204	a significantly lower risk	2179:2204	a significantly lower risk of gastrointestinal bleeding	2179:2233	However, taking a NOAC was associated with a significantly lower risk of gastrointestinal bleeding compared with receiving enoxaparin (10 patients [1.9%] vs 41 patients [7.1%]; HR, 0.29; 95% CI, 0.15-0.59; P < .001).
33533929	2	130	theme	bleeding	506:513	arg1	risk					462:465	the risk	458:465	the risk of recurrent thromboembolic events and bleeding associated with use of a NOAC vs use of the LMWH enoxaparin in Asian individuals with cancer-associated VTE	458:621	Objective To compare the risk of recurrent thromboembolic events and bleeding associated with use of a NOAC vs use of the LMWH enoxaparin in Asian individuals with cancer-associated VTE.
33533929	14	131	dep	%	2309:2309	arg1	P < .001					2342:2349	P < .001	2342:2349	P < .001	2342:2349	However, taking a NOAC was associated with a significantly lower risk of gastrointestinal bleeding compared with receiving enoxaparin (10 patients [1.9%] vs 41 patients [7.1%]; HR, 0.29; 95% CI, 0.15-0.59; P < .001).
33533929	14	131	dep	%	2309:2309	arg1	HR					2313:2314	HR	2313:2314	HR	2313:2314	However, taking a NOAC was associated with a significantly lower risk of gastrointestinal bleeding compared with receiving enoxaparin (10 patients [1.9%] vs 41 patients [7.1%]; HR, 0.29; 95% CI, 0.15-0.59; P < .001).
33533929	14	131	dep	%	2309:2309	arg1	0.15-0.59					2331:2339	0.15-0.59	2331:2339	0.15-0.59	2331:2339	However, taking a NOAC was associated with a significantly lower risk of gastrointestinal bleeding compared with receiving enoxaparin (10 patients [1.9%] vs 41 patients [7.1%]; HR, 0.29; 95% CI, 0.15-0.59; P < .001).
33533929	7	132	theme	primary	1124:1130	arg1	outcomes					1132:1139	The primary outcomes	1120:1139	The primary outcomes	1120:1139	Main Outcomes and Measures The primary outcomes were composite recurrent VTE or major bleeding.
33533929	9	133	theme	hazards	1375:1381	arg1	models					1383:1388	Cox proportional hazards models	1358:1388	Cox proportional hazards models	1358:1388	We compared risks of recurrent VTE or major bleeding between groups using Cox proportional hazards models.
33533929	2	134	theme	events	495:500	arg1	risk					462:465	the risk	458:465	the risk of recurrent thromboembolic events and bleeding associated with use of a NOAC vs use of the LMWH enoxaparin in Asian individuals with cancer-associated VTE	458:621	Objective To compare the risk of recurrent thromboembolic events and bleeding associated with use of a NOAC vs use of the LMWH enoxaparin in Asian individuals with cancer-associated VTE.
33533929	11	135	with	patients	1542:1549	arg1	VTE					1583:1585	newly diagnosed VTE	1567:1585	newly diagnosed VTE	1567:1585	Results Among 1109 patients with cancer and newly diagnosed VTE, 578 (52.1%) were women and the mean (SD) age at index date was 66.0 (13.0) years; 529 patients (47.7%) received NOACs and 580 patients (52.3%) received the LMWH enoxaparin.
33533929	11	135	with	patients	1542:1549	arg1	cancer					1556:1561	cancer	1556:1561	cancer	1556:1561	Results Among 1109 patients with cancer and newly diagnosed VTE, 578 (52.1%) were women and the mean (SD) age at index date was 66.0 (13.0) years; 529 patients (47.7%) received NOACs and 580 patients (52.3%) received the LMWH enoxaparin.
33533929	11	136	theme	LMWH	1744:1747	arg1	enoxaparin					1749:1758	the LMWH enoxaparin	1740:1758	the LMWH enoxaparin	1740:1758	Results Among 1109 patients with cancer and newly diagnosed VTE, 578 (52.1%) were women and the mean (SD) age at index date was 66.0 (13.0) years; 529 patients (47.7%) received NOACs and 580 patients (52.3%) received the LMWH enoxaparin.
33533929	13	137	theme	VTE	1997:1999	arg1	recurrence					2001:2010	VTE recurrence	1997:2010	VTE recurrence (HR, 0.62; 95% CI, 0.39-1.01; P = .05)	1997:2049	The groups had similar risk of VTE recurrence (HR, 0.62; 95% CI, 0.39-1.01; P = .05) and major bleeding (HR, 0.80; 95% CI, 0.52-1.24; P = .32) at 12 months of follow-up.
33533929	14	138	theme	lower	2195:2199	arg1	risk					2201:2204	a significantly lower risk	2179:2204	a significantly lower risk of gastrointestinal bleeding	2179:2233	However, taking a NOAC was associated with a significantly lower risk of gastrointestinal bleeding compared with receiving enoxaparin (10 patients [1.9%] vs 41 patients [7.1%]; HR, 0.29; 95% CI, 0.15-0.59; P < .001).
33533929	13	139	theme	0.80	2075:2078	arg1	HR					2071:2072	HR	2071:2072	HR	2071:2072	The groups had similar risk of VTE recurrence (HR, 0.62; 95% CI, 0.39-1.01; P = .05) and major bleeding (HR, 0.80; 95% CI, 0.52-1.24; P = .32) at 12 months of follow-up.
33533929	14	140	theme	%	2325:2325	arg1	CI					2327:2328	95% CI	2323:2328	95% CI	2323:2328	However, taking a NOAC was associated with a significantly lower risk of gastrointestinal bleeding compared with receiving enoxaparin (10 patients [1.9%] vs 41 patients [7.1%]; HR, 0.29; 95% CI, 0.15-0.59; P < .001).
33533929	10	141	theme	competing	1507:1515	arg1	risk					1517:1520	a competing risk	1505:1520	a competing risk	1505:1520	In addition, we conducted an analysis using a Fine and Gray subdistribution hazard model that considered death as a competing risk.
33533929	10	141	theme	competing	1507:1515	arg1	death					1496:1500	death	1496:1500	death	1496:1500	In addition, we conducted an analysis using a Fine and Gray subdistribution hazard model that considered death as a competing risk.
33533929	14	142	theme	0.29	2317:2320	arg1	HR					2313:2314	HR	2313:2314	HR	2313:2314	However, taking a NOAC was associated with a significantly lower risk of gastrointestinal bleeding compared with receiving enoxaparin (10 patients [1.9%] vs 41 patients [7.1%]; HR, 0.29; 95% CI, 0.15-0.59; P < .001).
33533929	15	143	dep	HR	2503:2504	arg1	P = .21					2532:2538	P = .21	2532:2538	P = .21	2532:2538	Findings for both primary outcomes were consistent with competing risk analyses (recurrent VTE: HR, 0.68; 95% CI, 0.45-1.01; P = .05; major bleeding: HR, 0.77; 95% CI, 0.51-1.16; P = .21).
33533929	15	143	dep	HR	2503:2504	arg1	%					2515:2515	95% CI, 0.51-1.16	2513:2529	%	2515:2515	Findings for both primary outcomes were consistent with competing risk analyses (recurrent VTE: HR, 0.68; 95% CI, 0.45-1.01; P = .05; major bleeding: HR, 0.77; 95% CI, 0.51-1.16; P = .21).
33533929	13	144	theme	95	2081:2082	arg1	%					2083:2083	%	2083:2083	%	2083:2083	The groups had similar risk of VTE recurrence (HR, 0.62; 95% CI, 0.39-1.01; P = .05) and major bleeding (HR, 0.80; 95% CI, 0.52-1.24; P = .32) at 12 months of follow-up.
33533929	3	145	theme	Gung	716:719	arg1	database					789:796	a multi-institutional electronic medical records database	740:796	a multi-institutional electronic medical records database in Taiwan	740:806	Design, Setting, and Participants This cohort study was conducted using data from the Chang Gung Research Database, a multi-institutional electronic medical records database in Taiwan.
33533929	3	145	theme	Gung	716:719	arg1	Database					730:737	the Chang Gung Research Database	706:737	the Chang Gung Research Database	706:737	Design, Setting, and Participants This cohort study was conducted using data from the Chang Gung Research Database, a multi-institutional electronic medical records database in Taiwan.
33533929	2	146	with	individuals	584:594	arg1	VTE					619:621	cancer-associated VTE	601:621	cancer-associated VTE	601:621	Objective To compare the risk of recurrent thromboembolic events and bleeding associated with use of a NOAC vs use of the LMWH enoxaparin in Asian individuals with cancer-associated VTE.
31924694	2	0	theme	N-glycosylation	619:633	arg1	functionality					589:601	the functionality	585:601	the functionality of differential N-glycosylation between sexes	585:647	Differences in protein glycosylation between sexes have already been observed in humans, nematodes and trematodes, and have recently also been reported in the rice pest insect Nilaparvata lugens Although protein N-glycosylation in insects is nowadays of high interest because of its potential for exploitation in pest control strategies, the functionality of differential N-glycosylation between sexes is yet unknown.
31924694	2	0	theme	N-glycosylation	619:633	arg1	unknown					656:662	unknown	656:662	unknown	656:662	Differences in protein glycosylation between sexes have already been observed in humans, nematodes and trematodes, and have recently also been reported in the rice pest insect Nilaparvata lugens Although protein N-glycosylation in insects is nowadays of high interest because of its potential for exploitation in pest control strategies, the functionality of differential N-glycosylation between sexes is yet unknown.
31924694	11	1	theme	original	2023:2030	arg1	data					2032:2035	original data	2023:2035	original data on N-glycosylation sites of N. lugens adults	2023:2080	Furthermore, original data on N-glycosylation sites of N. lugens adults are presented, providing novel insights into planthopper's biology and information for future biological pest control strategies.
31924694	6	2	from	differences	1329:1339	arg1	N-glycosylation					1352:1366	protein N-glycosylation	1344:1366	protein N-glycosylation	1344:1366	Comparison of these N-glycopeptides revealed striking differences in protein N-glycosylation between sexes.
31924694	9	3	theme	complete	1783:1790	arg1	insects					1792:1798	complete insects	1783:1798	complete insects	1783:1798	Both lectin blotting experiments as well as transcript expression analyses with complete insects and insect tissues confirmed the observed differences in N-glycosylation of proteins between sexes.
31924694	7	4	theme	N-glycosylation	1479:1493	arg1	sites					1495:1499	these sex-specific N-glycosylation sites	1460:1499	these sex-specific N-glycosylation sites	1460:1499	Male- and female-specific N-glycosylation sites were identified, and some of these sex-specific N-glycosylation sites were shown to be derived from proteins with a putative role in insect reproduction.
31924694	3	5	theme	protein	730:736	arg1	N-glycosylation					738:752	sex-related protein N-glycosylation	718:752	sex-related protein N-glycosylation	718:752	In this study, therefore, the occurrence and role of sex-related protein N-glycosylation in insects were examined.
31924694	11	6	theme	future	2169:2174	arg1	strategies					2200:2209	future biological pest control strategies	2169:2209	future biological pest control strategies	2169:2209	Furthermore, original data on N-glycosylation sites of N. lugens adults are presented, providing novel insights into planthopper's biology and information for future biological pest control strategies.
31924694	4	7	theme	N.	864:865	arg1	lugens					867:872	N. lugens	864:872	N. lugens	864:872	A comprehensive investigation of the N-glycosylation sites from the adult stages of N. lugens was conducted, allowing a qualitative and quantitative comparison between sexes at the glycopeptide level.
31924694	11	8	theme	N-glycosylation	2040:2054	arg1	adults					2075:2080	N. lugens adults	2065:2080	N. lugens adults	2065:2080	Furthermore, original data on N-glycosylation sites of N. lugens adults are presented, providing novel insights into planthopper's biology and information for future biological pest control strategies.
31924694	11	8	theme	N-glycosylation	2040:2054	arg1	sites					2056:2060	N-glycosylation sites	2040:2060	N-glycosylation sites of N. lugens adults	2040:2080	Furthermore, original data on N-glycosylation sites of N. lugens adults are presented, providing novel insights into planthopper's biology and information for future biological pest control strategies.
31924694	9	9	dep	experiments	1724:1734	arg1	Both					1703:1706	Both	1703:1706	Both	1703:1706	Both lectin blotting experiments as well as transcript expression analyses with complete insects and insect tissues confirmed the observed differences in N-glycosylation of proteins between sexes.
31924694	6	10	gly	N-glycopeptides	1295:1309	arg2	N-glycopeptides					1295:1309	these N-glycopeptides	1289:1309	these N-glycopeptides	1289:1309	Comparison of these N-glycopeptides revealed striking differences in protein N-glycosylation between sexes.
31924694	6	11	theme	protein	1344:1350	arg1	N-glycosylation					1352:1366	protein N-glycosylation	1344:1366	protein N-glycosylation	1344:1366	Comparison of these N-glycopeptides revealed striking differences in protein N-glycosylation between sexes.
31924694	1	12	theme	proteins	183:190	arg1	Glycosylation					141:153	Glycosylation	141:153	Glycosylation	141:153	Glycosylation is a common modification of proteins and critical for a wide range of biological processes.
31924694	1	12	theme	proteins	183:190	arg1	modification					167:178	a common modification	158:178	a common modification of proteins	158:190	Glycosylation is a common modification of proteins and critical for a wide range of biological processes.
31924694	7	13	from	role	1556:1559	arg1	reproduction					1571:1582	insect reproduction	1564:1582	insect reproduction	1564:1582	Male- and female-specific N-glycosylation sites were identified, and some of these sex-specific N-glycosylation sites were shown to be derived from proteins with a putative role in insect reproduction.
31924694	2	14	theme	pest	560:563	arg1	strategies					573:582	pest control strategies	560:582	pest control strategies	560:582	Differences in protein glycosylation between sexes have already been observed in humans, nematodes and trematodes, and have recently also been reported in the rice pest insect Nilaparvata lugens Although protein N-glycosylation in insects is nowadays of high interest because of its potential for exploitation in pest control strategies, the functionality of differential N-glycosylation between sexes is yet unknown.
31924694	10	15	theme	further	1935:1941	arg1	evidence					1943:1950	further evidence	1935:1950	further evidence for protein N-glycosylation	1935:1978	In conclusion, this study provides further evidence for protein N-glycosylation to be sex-related in insects.
31924694	11	16	theme	pest	2187:2190	arg1	strategies					2200:2209	future biological pest control strategies	2169:2209	future biological pest control strategies	2169:2209	Furthermore, original data on N-glycosylation sites of N. lugens adults are presented, providing novel insights into planthopper's biology and information for future biological pest control strategies.
31924694	6	17	theme	striking	1320:1327	arg1	differences					1329:1339	striking differences	1320:1339	striking differences in protein N-glycosylation between sexes	1320:1380	Comparison of these N-glycopeptides revealed striking differences in protein N-glycosylation between sexes.
31924694	3	18	from	occurrence	695:704	arg1	insects					757:763	insects	757:763	insects	757:763	In this study, therefore, the occurrence and role of sex-related protein N-glycosylation in insects were examined.
31924694	4	19	from	stages	854:859	arg1	sites					833:837	the N-glycosylation sites	813:837	the N-glycosylation sites from the adult stages of N. lugens	813:872	A comprehensive investigation of the N-glycosylation sites from the adult stages of N. lugens was conducted, allowing a qualitative and quantitative comparison between sexes at the glycopeptide level.
31924694	4	19	from	stages	854:859	arg1	investigation					796:808	A comprehensive investigation	780:808	A comprehensive investigation of the N-glycosylation sites from the adult stages of N. lugens	780:872	A comprehensive investigation of the N-glycosylation sites from the adult stages of N. lugens was conducted, allowing a qualitative and quantitative comparison between sexes at the glycopeptide level.
31924694	0	20	from	N-glycosylation	73:87	arg1	Planthopper					107:117	Planthopper	107:117	Planthopper	107:117	N-glycosylation Site Analysis Reveals Sex-related Differences in Protein N-glycosylation in the Rice Brown Planthopper (Nilaparvata lugens).
31924694	0	20	from	N-glycosylation	73:87	arg1	Nilaparvata					120:130	Nilaparvata	120:130	Nilaparvata	120:130	N-glycosylation Site Analysis Reveals Sex-related Differences in Protein N-glycosylation in the Rice Brown Planthopper (Nilaparvata lugens).
31924694	6	21	theme	N-glycopeptides	1295:1309	arg1	Comparison					1275:1284	Comparison	1275:1284	Comparison of these N-glycopeptides	1275:1309	Comparison of these N-glycopeptides revealed striking differences in protein N-glycosylation between sexes.
31924694	3	22	from	role	710:713	arg1	insects					757:763	insects	757:763	insects	757:763	In this study, therefore, the occurrence and role of sex-related protein N-glycosylation in insects were examined.
31924694	9	23	theme	transcript	1747:1756	arg1	analyses					1769:1776	transcript expression analyses	1747:1776	transcript expression analyses	1747:1776	Both lectin blotting experiments as well as transcript expression analyses with complete insects and insect tissues confirmed the observed differences in N-glycosylation of proteins between sexes.
31924694	9	24	theme	proteins	1876:1883	arg1	N-glycosylation					1857:1871	N-glycosylation	1857:1871	N-glycosylation of proteins	1857:1883	Both lectin blotting experiments as well as transcript expression analyses with complete insects and insect tissues confirmed the observed differences in N-glycosylation of proteins between sexes.
31924694	5	25	theme	Rhizoctonia	1102:1112	arg1	agglutinin					1121:1130	the Rhizoctonia solani agglutinin	1098:1130	the Rhizoctonia solani agglutinin which interacts with complex N-glycans	1098:1169	N-glycopeptide enrichment via lectin capturing using the high mannose/paucimannose-binding lectin Concanavalin A, or the Rhizoctonia solani agglutinin which interacts with complex N-glycans, resulted in the identification of over 1300 N-glycosylation sites derived from over 600 glycoproteins.
31924694	2	26	theme	protein	262:268	arg1	glycosylation					270:282	protein glycosylation	262:282	protein glycosylation	262:282	Differences in protein glycosylation between sexes have already been observed in humans, nematodes and trematodes, and have recently also been reported in the rice pest insect Nilaparvata lugens Although protein N-glycosylation in insects is nowadays of high interest because of its potential for exploitation in pest control strategies, the functionality of differential N-glycosylation between sexes is yet unknown.
31924694	8	27	theme	differential	1598:1609	arg1	composition					1618:1628	differential glycan composition	1598:1628	differential glycan composition between males and females	1598:1654	In addition, differential glycan composition between males and females was observed for proteins shared across sexes.
31924694	5	28	gly	N-glycosylation	1216:1230	arg2	sites					1232:1236	over 1300 N-glycosylation sites	1206:1236	over 1300 N-glycosylation sites derived from over 600 glycoproteins	1206:1272	N-glycopeptide enrichment via lectin capturing using the high mannose/paucimannose-binding lectin Concanavalin A, or the Rhizoctonia solani agglutinin which interacts with complex N-glycans, resulted in the identification of over 1300 N-glycosylation sites derived from over 600 glycoproteins.
31924694	5	28	gly	N-glycosylation	1216:1230	arg2	1300					1211:1214	1300	1211:1214	1300	1211:1214	N-glycopeptide enrichment via lectin capturing using the high mannose/paucimannose-binding lectin Concanavalin A, or the Rhizoctonia solani agglutinin which interacts with complex N-glycans, resulted in the identification of over 1300 N-glycosylation sites derived from over 600 glycoproteins.
31924694	7	29	gly	N-glycosylation	1409:1423	arg2	sites					1425:1429	Male- and female-specific N-glycosylation sites	1383:1429	Male- and female-specific N-glycosylation sites	1383:1429	Male- and female-specific N-glycosylation sites were identified, and some of these sex-specific N-glycosylation sites were shown to be derived from proteins with a putative role in insect reproduction.
31924694	4	30	from	investigation	796:808	arg1	stages					854:859	the adult stages	844:859	the adult stages of N. lugens	844:872	A comprehensive investigation of the N-glycosylation sites from the adult stages of N. lugens was conducted, allowing a qualitative and quantitative comparison between sexes at the glycopeptide level.
31924694	5	31	theme	complex	1153:1159	arg1	N-glycans					1161:1169	complex N-glycans	1153:1169	complex N-glycans	1153:1169	N-glycopeptide enrichment via lectin capturing using the high mannose/paucimannose-binding lectin Concanavalin A, or the Rhizoctonia solani agglutinin which interacts with complex N-glycans, resulted in the identification of over 1300 N-glycosylation sites derived from over 600 glycoproteins.
31924694	5	32	attach	derived	1238:1244	arg1	glycoproteins					1260:1272	over 600 glycoproteins	1251:1272	over 600 glycoproteins	1251:1272	N-glycopeptide enrichment via lectin capturing using the high mannose/paucimannose-binding lectin Concanavalin A, or the Rhizoctonia solani agglutinin which interacts with complex N-glycans, resulted in the identification of over 1300 N-glycosylation sites derived from over 600 glycoproteins.
31924694	5	32	attach	derived	1238:1244	arg2	sites					1232:1236	over 1300 N-glycosylation sites	1206:1236	over 1300 N-glycosylation sites derived from over 600 glycoproteins	1206:1272	N-glycopeptide enrichment via lectin capturing using the high mannose/paucimannose-binding lectin Concanavalin A, or the Rhizoctonia solani agglutinin which interacts with complex N-glycans, resulted in the identification of over 1300 N-glycosylation sites derived from over 600 glycoproteins.
31924694	5	33	gly	N-glycopeptide	981:994	arg2	N-glycopeptide					981:994	N-glycopeptide	981:994	N-glycopeptide	981:994	N-glycopeptide enrichment via lectin capturing using the high mannose/paucimannose-binding lectin Concanavalin A, or the Rhizoctonia solani agglutinin which interacts with complex N-glycans, resulted in the identification of over 1300 N-glycosylation sites derived from over 600 glycoproteins.
31924694	2	34	from	N-glycosylation	459:473	arg1	insects					478:484	insects	478:484	insects	478:484	Differences in protein glycosylation between sexes have already been observed in humans, nematodes and trematodes, and have recently also been reported in the rice pest insect Nilaparvata lugens Although protein N-glycosylation in insects is nowadays of high interest because of its potential for exploitation in pest control strategies, the functionality of differential N-glycosylation between sexes is yet unknown.
31924694	2	35	theme	protein	451:457	arg1	N-glycosylation					459:473	protein N-glycosylation	451:473	protein N-glycosylation in insects	451:484	Differences in protein glycosylation between sexes have already been observed in humans, nematodes and trematodes, and have recently also been reported in the rice pest insect Nilaparvata lugens Although protein N-glycosylation in insects is nowadays of high interest because of its potential for exploitation in pest control strategies, the functionality of differential N-glycosylation between sexes is yet unknown.
31924694	11	36	gly	N-glycosylation	2040:2054	arg1	adults					2075:2080	N. lugens adults	2065:2080	N. lugens adults	2065:2080	Furthermore, original data on N-glycosylation sites of N. lugens adults are presented, providing novel insights into planthopper's biology and information for future biological pest control strategies.
31924694	11	36	gly	N-glycosylation	2040:2054	arg2	adults					2075:2080	N. lugens adults	2065:2080	N. lugens adults	2065:2080	Furthermore, original data on N-glycosylation sites of N. lugens adults are presented, providing novel insights into planthopper's biology and information for future biological pest control strategies.
31924694	11	36	gly	N-glycosylation	2040:2054	arg2	sites					2056:2060	N-glycosylation sites	2040:2060	N-glycosylation sites of N. lugens adults	2040:2080	Furthermore, original data on N-glycosylation sites of N. lugens adults are presented, providing novel insights into planthopper's biology and information for future biological pest control strategies.
31924694	5	37	gly	glycoproteins	1260:1272	arg1	glycoproteins					1260:1272	over 600 glycoproteins	1251:1272	over 600 glycoproteins	1251:1272	N-glycopeptide enrichment via lectin capturing using the high mannose/paucimannose-binding lectin Concanavalin A, or the Rhizoctonia solani agglutinin which interacts with complex N-glycans, resulted in the identification of over 1300 N-glycosylation sites derived from over 600 glycoproteins.
31924694	9	38	theme	lectin	1708:1713	arg1	experiments					1724:1734	lectin blotting experiments	1708:1734	lectin blotting experiments	1708:1734	Both lectin blotting experiments as well as transcript expression analyses with complete insects and insect tissues confirmed the observed differences in N-glycosylation of proteins between sexes.
31924694	0	39	theme	N-glycosylation	0:14	arg1	Analysis					21:28	N-glycosylation Site Analysis	0:28	N-glycosylation Site Analysis	0:28	N-glycosylation Site Analysis Reveals Sex-related Differences in Protein N-glycosylation in the Rice Brown Planthopper (Nilaparvata lugens).
31924694	0	40	gly	N-glycosylation	0:14	arg2	Site					16:19	N-glycosylation Site Analysis	0:28	N-glycosylation Site Analysis	0:28	N-glycosylation Site Analysis Reveals Sex-related Differences in Protein N-glycosylation in the Rice Brown Planthopper (Nilaparvata lugens).
31924694	0	40	gly	N-glycosylation	0:14	arg2	Analysis					21:28	N-glycosylation Site Analysis	0:28	N-glycosylation Site Analysis	0:28	N-glycosylation Site Analysis Reveals Sex-related Differences in Protein N-glycosylation in the Rice Brown Planthopper (Nilaparvata lugens).
31924694	2	41	theme	insect	416:421	arg1	lugens					435:440	the rice pest insect Nilaparvata lugens	402:440	the rice pest insect Nilaparvata lugens	402:440	Differences in protein glycosylation between sexes have already been observed in humans, nematodes and trematodes, and have recently also been reported in the rice pest insect Nilaparvata lugens Although protein N-glycosylation in insects is nowadays of high interest because of its potential for exploitation in pest control strategies, the functionality of differential N-glycosylation between sexes is yet unknown.
31924694	5	42	theme	N-glycopeptide	981:994	arg1	enrichment					996:1005	N-glycopeptide enrichment	981:1005	N-glycopeptide enrichment	981:1005	N-glycopeptide enrichment via lectin capturing using the high mannose/paucimannose-binding lectin Concanavalin A, or the Rhizoctonia solani agglutinin which interacts with complex N-glycans, resulted in the identification of over 1300 N-glycosylation sites derived from over 600 glycoproteins.
31924694	10	43	from	sex-related	1986:1996	arg1	insects					2001:2007	insects	2001:2007	insects	2001:2007	In conclusion, this study provides further evidence for protein N-glycosylation to be sex-related in insects.
31924694	2	44	located	observed	316:323	arg1	nematodes					336:344	nematodes	336:344	nematodes	336:344	Differences in protein glycosylation between sexes have already been observed in humans, nematodes and trematodes, and have recently also been reported in the rice pest insect Nilaparvata lugens Although protein N-glycosylation in insects is nowadays of high interest because of its potential for exploitation in pest control strategies, the functionality of differential N-glycosylation between sexes is yet unknown.
31924694	2	44	located	observed	316:323	arg1	trematodes					350:359	trematodes	350:359	trematodes	350:359	Differences in protein glycosylation between sexes have already been observed in humans, nematodes and trematodes, and have recently also been reported in the rice pest insect Nilaparvata lugens Although protein N-glycosylation in insects is nowadays of high interest because of its potential for exploitation in pest control strategies, the functionality of differential N-glycosylation between sexes is yet unknown.
31924694	2	44	located	observed	316:323	arg2	Differences					247:257	Differences	247:257	Differences in protein glycosylation between sexes	247:296	Differences in protein glycosylation between sexes have already been observed in humans, nematodes and trematodes, and have recently also been reported in the rice pest insect Nilaparvata lugens Although protein N-glycosylation in insects is nowadays of high interest because of its potential for exploitation in pest control strategies, the functionality of differential N-glycosylation between sexes is yet unknown.
31924694	2	44	located	observed	316:323	arg1	humans					328:333	humans	328:333	humans	328:333	Differences in protein glycosylation between sexes have already been observed in humans, nematodes and trematodes, and have recently also been reported in the rice pest insect Nilaparvata lugens Although protein N-glycosylation in insects is nowadays of high interest because of its potential for exploitation in pest control strategies, the functionality of differential N-glycosylation between sexes is yet unknown.
31924694	11	45	theme	lugens	2068:2073	arg1	adults					2075:2080	N. lugens adults	2065:2080	N. lugens adults	2065:2080	Furthermore, original data on N-glycosylation sites of N. lugens adults are presented, providing novel insights into planthopper's biology and information for future biological pest control strategies.
31924694	2	46	theme	rice	406:409	arg1	lugens					435:440	the rice pest insect Nilaparvata lugens	402:440	the rice pest insect Nilaparvata lugens	402:440	Differences in protein glycosylation between sexes have already been observed in humans, nematodes and trematodes, and have recently also been reported in the rice pest insect Nilaparvata lugens Although protein N-glycosylation in insects is nowadays of high interest because of its potential for exploitation in pest control strategies, the functionality of differential N-glycosylation between sexes is yet unknown.
31924694	0	47	theme	Sex-related	38:48	arg1	Differences					50:60	Sex-related Differences	38:60	Sex-related Differences in Protein N-glycosylation in the Rice Brown Planthopper (Nilaparvata lugens)	38:138	N-glycosylation Site Analysis Reveals Sex-related Differences in Protein N-glycosylation in the Rice Brown Planthopper (Nilaparvata lugens).
31924694	5	48	theme	Concanavalin	1079:1090	arg1	A					1092:1092	the high mannose/paucimannose-binding lectin Concanavalin A	1034:1092	the high mannose/paucimannose-binding lectin Concanavalin A	1034:1092	N-glycopeptide enrichment via lectin capturing using the high mannose/paucimannose-binding lectin Concanavalin A, or the Rhizoctonia solani agglutinin which interacts with complex N-glycans, resulted in the identification of over 1300 N-glycosylation sites derived from over 600 glycoproteins.
31924694	8	49	located	observed	1660:1667	arg1	addition					1588:1595	addition	1588:1595	addition	1588:1595	In addition, differential glycan composition between males and females was observed for proteins shared across sexes.
31924694	8	49	located	observed	1660:1667	arg2	composition					1618:1628	differential glycan composition	1598:1628	differential glycan composition between males and females	1598:1654	In addition, differential glycan composition between males and females was observed for proteins shared across sexes.
31924694	7	50	theme	female-specific	1393:1407	arg1	sites					1425:1429	Male- and female-specific N-glycosylation sites	1383:1429	Male- and female-specific N-glycosylation sites	1383:1429	Male- and female-specific N-glycosylation sites were identified, and some of these sex-specific N-glycosylation sites were shown to be derived from proteins with a putative role in insect reproduction.
31924694	5	51	theme	N-glycosylation	1216:1230	arg1	sites					1232:1236	over 1300 N-glycosylation sites	1206:1236	over 1300 N-glycosylation sites derived from over 600 glycoproteins	1206:1272	N-glycopeptide enrichment via lectin capturing using the high mannose/paucimannose-binding lectin Concanavalin A, or the Rhizoctonia solani agglutinin which interacts with complex N-glycans, resulted in the identification of over 1300 N-glycosylation sites derived from over 600 glycoproteins.
31924694	2	52	dep	nowadays	489:496	arg1	unknown					656:662	unknown	656:662	unknown	656:662	Differences in protein glycosylation between sexes have already been observed in humans, nematodes and trematodes, and have recently also been reported in the rice pest insect Nilaparvata lugens Although protein N-glycosylation in insects is nowadays of high interest because of its potential for exploitation in pest control strategies, the functionality of differential N-glycosylation between sexes is yet unknown.
31924694	2	52	dep	nowadays	489:496	arg1	functionality					589:601	the functionality	585:601	the functionality of differential N-glycosylation between sexes	585:647	Differences in protein glycosylation between sexes have already been observed in humans, nematodes and trematodes, and have recently also been reported in the rice pest insect Nilaparvata lugens Although protein N-glycosylation in insects is nowadays of high interest because of its potential for exploitation in pest control strategies, the functionality of differential N-glycosylation between sexes is yet unknown.
31924694	7	53	theme	Male-	1383:1387	arg1	sites					1425:1429	Male- and female-specific N-glycosylation sites	1383:1429	Male- and female-specific N-glycosylation sites	1383:1429	Male- and female-specific N-glycosylation sites were identified, and some of these sex-specific N-glycosylation sites were shown to be derived from proteins with a putative role in insect reproduction.
31924694	9	54	theme	blotting	1715:1722	arg1	experiments					1724:1734	lectin blotting experiments	1708:1734	lectin blotting experiments	1708:1734	Both lectin blotting experiments as well as transcript expression analyses with complete insects and insect tissues confirmed the observed differences in N-glycosylation of proteins between sexes.
31924694	11	55	theme	novel	2107:2111	arg1	insights					2113:2120	novel insights	2107:2120	novel insights into planthopper's biology and information for future biological pest control strategies	2107:2209	Furthermore, original data on N-glycosylation sites of N. lugens adults are presented, providing novel insights into planthopper's biology and information for future biological pest control strategies.
31924694	9	56	theme	expression	1758:1767	arg1	analyses					1769:1776	transcript expression analyses	1747:1776	transcript expression analyses	1747:1776	Both lectin blotting experiments as well as transcript expression analyses with complete insects and insect tissues confirmed the observed differences in N-glycosylation of proteins between sexes.
31924694	4	57	theme	sites	833:837	arg1	investigation					796:808	A comprehensive investigation	780:808	A comprehensive investigation of the N-glycosylation sites from the adult stages of N. lugens	780:872	A comprehensive investigation of the N-glycosylation sites from the adult stages of N. lugens was conducted, allowing a qualitative and quantitative comparison between sexes at the glycopeptide level.
31924694	1	58	theme	processes	236:244	arg1	range					216:220	a wide range	209:220	a wide range of biological processes	209:244	Glycosylation is a common modification of proteins and critical for a wide range of biological processes.
31924694	0	59	theme	Protein	65:71	arg1	N-glycosylation					73:87	Protein N-glycosylation	65:87	Protein N-glycosylation in the Rice Brown Planthopper (Nilaparvata lugens)	65:138	N-glycosylation Site Analysis Reveals Sex-related Differences in Protein N-glycosylation in the Rice Brown Planthopper (Nilaparvata lugens).
31924694	5	60	theme	mannose/paucimannose-binding	1043:1070	arg1	A					1092:1092	the high mannose/paucimannose-binding lectin Concanavalin A	1034:1092	the high mannose/paucimannose-binding lectin Concanavalin A	1034:1092	N-glycopeptide enrichment via lectin capturing using the high mannose/paucimannose-binding lectin Concanavalin A, or the Rhizoctonia solani agglutinin which interacts with complex N-glycans, resulted in the identification of over 1300 N-glycosylation sites derived from over 600 glycoproteins.
31924694	9	61	with	analyses	1769:1776	arg1	insects					1792:1798	complete insects	1783:1798	complete insects	1783:1798	Both lectin blotting experiments as well as transcript expression analyses with complete insects and insect tissues confirmed the observed differences in N-glycosylation of proteins between sexes.
31924694	9	61	with	analyses	1769:1776	arg1	tissues					1811:1817	insect tissues	1804:1817	insect tissues	1804:1817	Both lectin blotting experiments as well as transcript expression analyses with complete insects and insect tissues confirmed the observed differences in N-glycosylation of proteins between sexes.
31924694	2	62	theme	differential	606:617	arg1	N-glycosylation					619:633	differential N-glycosylation	606:633	differential N-glycosylation between sexes	606:647	Differences in protein glycosylation between sexes have already been observed in humans, nematodes and trematodes, and have recently also been reported in the rice pest insect Nilaparvata lugens Although protein N-glycosylation in insects is nowadays of high interest because of its potential for exploitation in pest control strategies, the functionality of differential N-glycosylation between sexes is yet unknown.
31924694	9	63	with	experiments	1724:1734	arg1	insects					1792:1798	complete insects	1783:1798	complete insects	1783:1798	Both lectin blotting experiments as well as transcript expression analyses with complete insects and insect tissues confirmed the observed differences in N-glycosylation of proteins between sexes.
31924694	9	63	with	experiments	1724:1734	arg1	tissues					1811:1817	insect tissues	1804:1817	insect tissues	1804:1817	Both lectin blotting experiments as well as transcript expression analyses with complete insects and insect tissues confirmed the observed differences in N-glycosylation of proteins between sexes.
31924694	4	64	from	level	974:978	arg1	sexes					948:952	sexes	948:952	sexes at the glycopeptide level	948:978	A comprehensive investigation of the N-glycosylation sites from the adult stages of N. lugens was conducted, allowing a qualitative and quantitative comparison between sexes at the glycopeptide level.
31924694	7	65	theme	insect	1564:1569	arg1	reproduction					1571:1582	insect reproduction	1564:1582	insect reproduction	1564:1582	Male- and female-specific N-glycosylation sites were identified, and some of these sex-specific N-glycosylation sites were shown to be derived from proteins with a putative role in insect reproduction.
31924694	7	66	theme	putative	1547:1554	arg1	role					1556:1559	a putative role	1545:1559	a putative role in insect reproduction	1545:1582	Male- and female-specific N-glycosylation sites were identified, and some of these sex-specific N-glycosylation sites were shown to be derived from proteins with a putative role in insect reproduction.
31924694	3	67	theme	N-glycosylation	738:752	arg1	role					710:713	role	710:713	role	710:713	In this study, therefore, the occurrence and role of sex-related protein N-glycosylation in insects were examined.
31924694	3	67	theme	N-glycosylation	738:752	arg1	occurrence					695:704	occurrence	695:704	occurrence	695:704	In this study, therefore, the occurrence and role of sex-related protein N-glycosylation in insects were examined.
31924694	9	68	theme	insect	1804:1809	arg1	tissues					1811:1817	insect tissues	1804:1817	insect tissues	1804:1817	Both lectin blotting experiments as well as transcript expression analyses with complete insects and insect tissues confirmed the observed differences in N-glycosylation of proteins between sexes.
31924694	3	69	theme	sex-related	718:728	arg1	N-glycosylation					738:752	sex-related protein N-glycosylation	718:752	sex-related protein N-glycosylation	718:752	In this study, therefore, the occurrence and role of sex-related protein N-glycosylation in insects were examined.
31924694	11	70	theme	control	2192:2198	arg1	strategies					2200:2209	future biological pest control strategies	2169:2209	future biological pest control strategies	2169:2209	Furthermore, original data on N-glycosylation sites of N. lugens adults are presented, providing novel insights into planthopper's biology and information for future biological pest control strategies.
31924694	4	71	theme	lugens	867:872	arg1	stages					854:859	the adult stages	844:859	the adult stages of N. lugens	844:872	A comprehensive investigation of the N-glycosylation sites from the adult stages of N. lugens was conducted, allowing a qualitative and quantitative comparison between sexes at the glycopeptide level.
31924694	7	72	attach	derived	1518:1524	arg2	some					1452:1455	some	1452:1455	some	1452:1455	Male- and female-specific N-glycosylation sites were identified, and some of these sex-specific N-glycosylation sites were shown to be derived from proteins with a putative role in insect reproduction.
31924694	7	72	attach	derived	1518:1524	arg2	sites					1495:1499	these sex-specific N-glycosylation sites	1460:1499	these sex-specific N-glycosylation sites	1460:1499	Male- and female-specific N-glycosylation sites were identified, and some of these sex-specific N-glycosylation sites were shown to be derived from proteins with a putative role in insect reproduction.
31924694	7	72	attach	derived	1518:1524	arg1	proteins					1531:1538	proteins	1531:1538	proteins with a putative role in insect reproduction	1531:1582	Male- and female-specific N-glycosylation sites were identified, and some of these sex-specific N-glycosylation sites were shown to be derived from proteins with a putative role in insect reproduction.
31924694	2	73	theme	control	565:571	arg1	strategies					573:582	pest control strategies	560:582	pest control strategies	560:582	Differences in protein glycosylation between sexes have already been observed in humans, nematodes and trematodes, and have recently also been reported in the rice pest insect Nilaparvata lugens Although protein N-glycosylation in insects is nowadays of high interest because of its potential for exploitation in pest control strategies, the functionality of differential N-glycosylation between sexes is yet unknown.
31924694	11	74	theme	biological	2176:2185	arg1	strategies					2200:2209	future biological pest control strategies	2169:2209	future biological pest control strategies	2169:2209	Furthermore, original data on N-glycosylation sites of N. lugens adults are presented, providing novel insights into planthopper's biology and information for future biological pest control strategies.
31924694	9	75	gly	N-glycosylation	1857:1871	arg1	proteins					1876:1883	proteins	1876:1883	proteins	1876:1883	Both lectin blotting experiments as well as transcript expression analyses with complete insects and insect tissues confirmed the observed differences in N-glycosylation of proteins between sexes.
31924694	9	76	theme	observed	1833:1840	arg1	differences					1842:1852	the observed differences	1829:1852	the observed differences in N-glycosylation of proteins between sexes	1829:1897	Both lectin blotting experiments as well as transcript expression analyses with complete insects and insect tissues confirmed the observed differences in N-glycosylation of proteins between sexes.
31924694	10	77	theme	protein	1956:1962	arg1	N-glycosylation					1964:1978	protein N-glycosylation	1956:1978	protein N-glycosylation	1956:1978	In conclusion, this study provides further evidence for protein N-glycosylation to be sex-related in insects.
31924694	1	78	theme	wide	211:214	arg1	range					216:220	a wide range	209:220	a wide range of biological processes	209:244	Glycosylation is a common modification of proteins and critical for a wide range of biological processes.
31924694	7	79	gly	N-glycosylation	1479:1493	arg2	sites					1495:1499	these sex-specific N-glycosylation sites	1460:1499	these sex-specific N-glycosylation sites	1460:1499	Male- and female-specific N-glycosylation sites were identified, and some of these sex-specific N-glycosylation sites were shown to be derived from proteins with a putative role in insect reproduction.
31924694	4	80	theme	qualitative	900:910	arg1	comparison					929:938	a qualitative and quantitative comparison	898:938	a qualitative and quantitative comparison between sexes at the glycopeptide level	898:978	A comprehensive investigation of the N-glycosylation sites from the adult stages of N. lugens was conducted, allowing a qualitative and quantitative comparison between sexes at the glycopeptide level.
31924694	0	81	gly	N-glycosylation	73:87	arg1	Planthopper					107:117	Planthopper	107:117	Planthopper	107:117	N-glycosylation Site Analysis Reveals Sex-related Differences in Protein N-glycosylation in the Rice Brown Planthopper (Nilaparvata lugens).
31924694	0	81	gly	N-glycosylation	73:87	arg1	Nilaparvata					120:130	Nilaparvata	120:130	Nilaparvata	120:130	N-glycosylation Site Analysis Reveals Sex-related Differences in Protein N-glycosylation in the Rice Brown Planthopper (Nilaparvata lugens).
31924694	4	82	theme	quantitative	916:927	arg1	comparison					929:938	a qualitative and quantitative comparison	898:938	a qualitative and quantitative comparison between sexes at the glycopeptide level	898:978	A comprehensive investigation of the N-glycosylation sites from the adult stages of N. lugens was conducted, allowing a qualitative and quantitative comparison between sexes at the glycopeptide level.
31924694	7	83	theme	sex-specific	1466:1477	arg1	sites					1495:1499	these sex-specific N-glycosylation sites	1460:1499	these sex-specific N-glycosylation sites	1460:1499	Male- and female-specific N-glycosylation sites were identified, and some of these sex-specific N-glycosylation sites were shown to be derived from proteins with a putative role in insect reproduction.
31924694	5	84	theme	solani	1114:1119	arg1	agglutinin					1121:1130	the Rhizoctonia solani agglutinin	1098:1130	the Rhizoctonia solani agglutinin which interacts with complex N-glycans	1098:1169	N-glycopeptide enrichment via lectin capturing using the high mannose/paucimannose-binding lectin Concanavalin A, or the Rhizoctonia solani agglutinin which interacts with complex N-glycans, resulted in the identification of over 1300 N-glycosylation sites derived from over 600 glycoproteins.
31924694	2	85	theme	high	501:504	arg1	interest					506:513	high interest	501:513	high interest	501:513	Differences in protein glycosylation between sexes have already been observed in humans, nematodes and trematodes, and have recently also been reported in the rice pest insect Nilaparvata lugens Although protein N-glycosylation in insects is nowadays of high interest because of its potential for exploitation in pest control strategies, the functionality of differential N-glycosylation between sexes is yet unknown.
31924694	8	86	theme	glycan	1611:1616	arg1	composition					1618:1628	differential glycan composition	1598:1628	differential glycan composition between males and females	1598:1654	In addition, differential glycan composition between males and females was observed for proteins shared across sexes.
31924694	1	87	theme	common	160:165	arg1	Glycosylation					141:153	Glycosylation	141:153	Glycosylation	141:153	Glycosylation is a common modification of proteins and critical for a wide range of biological processes.
31924694	1	87	theme	common	160:165	arg1	modification					167:178	a common modification	158:178	a common modification of proteins	158:190	Glycosylation is a common modification of proteins and critical for a wide range of biological processes.
31924694	9	88	from	differences	1842:1852	arg1	N-glycosylation					1857:1871	N-glycosylation	1857:1871	N-glycosylation of proteins	1857:1883	Both lectin blotting experiments as well as transcript expression analyses with complete insects and insect tissues confirmed the observed differences in N-glycosylation of proteins between sexes.
31924694	0	89	dep	Nilaparvata	120:130	arg1	lugens					132:137	Nilaparvata lugens	120:137	Nilaparvata lugens	120:137	N-glycosylation Site Analysis Reveals Sex-related Differences in Protein N-glycosylation in the Rice Brown Planthopper (Nilaparvata lugens).
31924694	4	90	theme	glycopeptide	961:972	arg1	level					974:978	the glycopeptide level	957:978	the glycopeptide level	957:978	A comprehensive investigation of the N-glycosylation sites from the adult stages of N. lugens was conducted, allowing a qualitative and quantitative comparison between sexes at the glycopeptide level.
31924694	11	91	from	data	2032:2035	arg1	adults					2075:2080	N. lugens adults	2065:2080	N. lugens adults	2065:2080	Furthermore, original data on N-glycosylation sites of N. lugens adults are presented, providing novel insights into planthopper's biology and information for future biological pest control strategies.
31924694	11	91	from	data	2032:2035	arg1	sites					2056:2060	N-glycosylation sites	2040:2060	N-glycosylation sites of N. lugens adults	2040:2080	Furthermore, original data on N-glycosylation sites of N. lugens adults are presented, providing novel insights into planthopper's biology and information for future biological pest control strategies.
31924694	5	92	theme	lectin	1011:1016	arg1	capturing					1018:1026	lectin capturing	1011:1026	lectin capturing using the high mannose/paucimannose-binding lectin Concanavalin A, or the Rhizoctonia solani agglutinin which interacts with complex N-glycans	1011:1169	N-glycopeptide enrichment via lectin capturing using the high mannose/paucimannose-binding lectin Concanavalin A, or the Rhizoctonia solani agglutinin which interacts with complex N-glycans, resulted in the identification of over 1300 N-glycosylation sites derived from over 600 glycoproteins.
31924694	0	93	from	Differences	50:60	arg1	N-glycosylation					73:87	Protein N-glycosylation	65:87	Protein N-glycosylation in the Rice Brown Planthopper (Nilaparvata lugens)	65:138	N-glycosylation Site Analysis Reveals Sex-related Differences in Protein N-glycosylation in the Rice Brown Planthopper (Nilaparvata lugens).
31924694	4	94	theme	comprehensive	782:794	arg1	investigation					796:808	A comprehensive investigation	780:808	A comprehensive investigation of the N-glycosylation sites from the adult stages of N. lugens	780:872	A comprehensive investigation of the N-glycosylation sites from the adult stages of N. lugens was conducted, allowing a qualitative and quantitative comparison between sexes at the glycopeptide level.
31924694	11	95	theme	N.	2065:2066	arg1	adults					2075:2080	N. lugens adults	2065:2080	N. lugens adults	2065:2080	Furthermore, original data on N-glycosylation sites of N. lugens adults are presented, providing novel insights into planthopper's biology and information for future biological pest control strategies.
31924694	0	96	theme	Site	16:19	arg1	Analysis					21:28	N-glycosylation Site Analysis	0:28	N-glycosylation Site Analysis	0:28	N-glycosylation Site Analysis Reveals Sex-related Differences in Protein N-glycosylation in the Rice Brown Planthopper (Nilaparvata lugens).
31924694	4	97	gly	glycopeptide	961:972	arg2	glycopeptide					961:972	the glycopeptide level	957:978	the glycopeptide level	957:978	A comprehensive investigation of the N-glycosylation sites from the adult stages of N. lugens was conducted, allowing a qualitative and quantitative comparison between sexes at the glycopeptide level.
31924694	3	98	dep	occurrence	695:704	arg1	the					691:693	the	691:693	the	691:693	In this study, therefore, the occurrence and role of sex-related protein N-glycosylation in insects were examined.
31924694	2	99	from	Differences	247:257	arg1	glycosylation					270:282	protein glycosylation	262:282	protein glycosylation	262:282	Differences in protein glycosylation between sexes have already been observed in humans, nematodes and trematodes, and have recently also been reported in the rice pest insect Nilaparvata lugens Although protein N-glycosylation in insects is nowadays of high interest because of its potential for exploitation in pest control strategies, the functionality of differential N-glycosylation between sexes is yet unknown.
31924694	7	100	theme	N-glycosylation	1409:1423	arg1	sites					1425:1429	Male- and female-specific N-glycosylation sites	1383:1429	Male- and female-specific N-glycosylation sites	1383:1429	Male- and female-specific N-glycosylation sites were identified, and some of these sex-specific N-glycosylation sites were shown to be derived from proteins with a putative role in insect reproduction.
31924694	2	101	theme	Nilaparvata	423:433	arg1	lugens					435:440	the rice pest insect Nilaparvata lugens	402:440	the rice pest insect Nilaparvata lugens	402:440	Differences in protein glycosylation between sexes have already been observed in humans, nematodes and trematodes, and have recently also been reported in the rice pest insect Nilaparvata lugens Although protein N-glycosylation in insects is nowadays of high interest because of its potential for exploitation in pest control strategies, the functionality of differential N-glycosylation between sexes is yet unknown.
31924694	2	102	theme	pest	411:414	arg1	lugens					435:440	the rice pest insect Nilaparvata lugens	402:440	the rice pest insect Nilaparvata lugens	402:440	Differences in protein glycosylation between sexes have already been observed in humans, nematodes and trematodes, and have recently also been reported in the rice pest insect Nilaparvata lugens Although protein N-glycosylation in insects is nowadays of high interest because of its potential for exploitation in pest control strategies, the functionality of differential N-glycosylation between sexes is yet unknown.
31924694	7	103	with	proteins	1531:1538	arg1	role					1556:1559	a putative role	1545:1559	a putative role in insect reproduction	1545:1582	Male- and female-specific N-glycosylation sites were identified, and some of these sex-specific N-glycosylation sites were shown to be derived from proteins with a putative role in insect reproduction.
31924694	5	104	theme	lectin	1072:1077	arg1	A					1092:1092	the high mannose/paucimannose-binding lectin Concanavalin A	1034:1092	the high mannose/paucimannose-binding lectin Concanavalin A	1034:1092	N-glycopeptide enrichment via lectin capturing using the high mannose/paucimannose-binding lectin Concanavalin A, or the Rhizoctonia solani agglutinin which interacts with complex N-glycans, resulted in the identification of over 1300 N-glycosylation sites derived from over 600 glycoproteins.
31924694	11	105	theme	adults	2075:2080	arg1	adults					2075:2080	N. lugens adults	2065:2080	N. lugens adults	2065:2080	Furthermore, original data on N-glycosylation sites of N. lugens adults are presented, providing novel insights into planthopper's biology and information for future biological pest control strategies.
31924694	11	105	theme	adults	2075:2080	arg1	sites					2056:2060	N-glycosylation sites	2040:2060	N-glycosylation sites of N. lugens adults	2040:2080	Furthermore, original data on N-glycosylation sites of N. lugens adults are presented, providing novel insights into planthopper's biology and information for future biological pest control strategies.
31924694	4	106	gly	N-glycosylation	817:831	arg2	sites					833:837	the N-glycosylation sites	813:837	the N-glycosylation sites from the adult stages of N. lugens	813:872	A comprehensive investigation of the N-glycosylation sites from the adult stages of N. lugens was conducted, allowing a qualitative and quantitative comparison between sexes at the glycopeptide level.
31924694	2	107	gly	N-glycosylation	459:473	arg1	insects					478:484	insects	478:484	insects	478:484	Differences in protein glycosylation between sexes have already been observed in humans, nematodes and trematodes, and have recently also been reported in the rice pest insect Nilaparvata lugens Although protein N-glycosylation in insects is nowadays of high interest because of its potential for exploitation in pest control strategies, the functionality of differential N-glycosylation between sexes is yet unknown.
31924694	4	108	theme	adult	848:852	arg1	stages					854:859	the adult stages	844:859	the adult stages of N. lugens	844:872	A comprehensive investigation of the N-glycosylation sites from the adult stages of N. lugens was conducted, allowing a qualitative and quantitative comparison between sexes at the glycopeptide level.
31924694	5	109	theme	sites	1232:1236	arg1	identification					1188:1201	the identification	1184:1201	the identification of over 1300 N-glycosylation sites derived from over 600 glycoproteins	1184:1272	N-glycopeptide enrichment via lectin capturing using the high mannose/paucimannose-binding lectin Concanavalin A, or the Rhizoctonia solani agglutinin which interacts with complex N-glycans, resulted in the identification of over 1300 N-glycosylation sites derived from over 600 glycoproteins.
31924694	2	110	from	exploitation	544:555	arg1	strategies					573:582	pest control strategies	560:582	pest control strategies	560:582	Differences in protein glycosylation between sexes have already been observed in humans, nematodes and trematodes, and have recently also been reported in the rice pest insect Nilaparvata lugens Although protein N-glycosylation in insects is nowadays of high interest because of its potential for exploitation in pest control strategies, the functionality of differential N-glycosylation between sexes is yet unknown.
31924694	1	111	theme	biological	225:234	arg1	processes					236:244	biological processes	225:244	biological processes	225:244	Glycosylation is a common modification of proteins and critical for a wide range of biological processes.
31924694	10	112	from	insects	2001:2007	arg1	sex-related					1986:1996	sex-related	1986:1996	sex-related	1986:1996	In conclusion, this study provides further evidence for protein N-glycosylation to be sex-related in insects.
31924694	5	113	theme	high	1038:1041	arg1	A					1092:1092	the high mannose/paucimannose-binding lectin Concanavalin A	1034:1092	the high mannose/paucimannose-binding lectin Concanavalin A	1034:1092	N-glycopeptide enrichment via lectin capturing using the high mannose/paucimannose-binding lectin Concanavalin A, or the Rhizoctonia solani agglutinin which interacts with complex N-glycans, resulted in the identification of over 1300 N-glycosylation sites derived from over 600 glycoproteins.
31924694	4	114	theme	N-glycosylation	817:831	arg1	sites					833:837	the N-glycosylation sites	813:837	the N-glycosylation sites from the adult stages of N. lugens	813:872	A comprehensive investigation of the N-glycosylation sites from the adult stages of N. lugens was conducted, allowing a qualitative and quantitative comparison between sexes at the glycopeptide level.
31622233	5	0	dep	%	591:591	arg1	%					614:614	0 %	612:614	1 % for strain V44-8T; 0 % for strain V47-23aT	589:634	Both strains displayed growth in 0-5 % NaCl with an optimum at 1 % for strain V44-8T; 0 % for strain V47-23aT.
31622233	12	1	theme	average	1461:1467	arg1	identity					1480:1487	average amino acid identity	1461:1487	average amino acid identity	1461:1487	Average nucleotide identity and average amino acid identity values between strains V44-8T and DSM-103964T were 72.1% and 67.5 %; V47-23aT and DSM-105484T were 62.4% and 69.1%, respectively.
31622233	5	2	theme	0-5 	559:562	arg1	%					563:563	%	563:563	%	563:563	Both strains displayed growth in 0-5 % NaCl with an optimum at 1 % for strain V44-8T; 0 % for strain V47-23aT.
31622233	9	3	dep	anteiso-C15 	974:985	arg1	 0					1000:1001	 0	1000:1001	 0	1000:1001	The predominant cellular fatty acids were anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0.
31622233	9	3	dep	anteiso-C15 	974:985	arg1	iso-C15 					1007:1014	iso-C15 	1007:1014	iso-C15 	1007:1014	The predominant cellular fatty acids were anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0.
31622233	9	3	dep	anteiso-C15 	974:985	arg1	 0					987:988	 0	987:988	 0	987:988	The predominant cellular fatty acids were anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0.
31622233	9	3	dep	anteiso-C15 	974:985	arg1	 0					1016:1017	 0	1016:1017	 0	1016:1017	The predominant cellular fatty acids were anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0.
31622233	11	4	theme	Bacillus	1358:1365	arg1	members					1337:1343	a members	1335:1343	a members of the genus Bacillus that forms a separate clade when compared to close relatives	1335:1426	Based on their phenotypic characteristics and phylogenetic position inferred from 16S rRNA gene sequence analyses, the isolates were identified as being a members of the genus Bacillus that forms a separate clade when compared to close relatives.
31622233	10	5	theme	rRNA	1059:1062	arg1	similarity					1073:1082	its highest 16S rRNA sequence similarity	1043:1082	its highest 16S rRNA sequence similarity with Bacillus cavernae DSM-105484T at 96.9%	1043:1126	Strain V47.23aT shared its highest 16S rRNA sequence similarity with Bacillus cavernae DSM-105484T at 96.9%, and V44.8T with Bacillus zeae DSM-103964T at 96.6 %.
31622233	13	6	theme	phenotypic	1632:1641	arg1	data					1668:1671	the phenotypic, genomic and biochemical data	1628:1671	the phenotypic, genomic and biochemical data	1628:1671	Based on the phenotypic, genomic and biochemical data, strains V44-8T and V47-23aT represent two novel species in the genus Bacillus for which the names Bacillus glennii sp.
31622233	5	7	theme	%	563:563	arg1	 NaCl					564:568	0-5 % NaCl	559:568	0-5 % NaCl	559:568	Both strains displayed growth in 0-5 % NaCl with an optimum at 1 % for strain V44-8T; 0 % for strain V47-23aT.
31622233	7	8	theme	meso-diaminopimelic	777:795	arg1	acid					828:831	the diagnostic diamino acid	805:831	the diagnostic diamino acid	805:831	The cell wall of both strains contained meso-diaminopimelic acid as the diagnostic diamino acid.
31622233	7	8	theme	meso-diaminopimelic	777:795	arg1	acid					797:800	meso-diaminopimelic acid	777:800	meso-diaminopimelic acid	777:800	The cell wall of both strains contained meso-diaminopimelic acid as the diagnostic diamino acid.
31622233	3	9	dep	Gram-stain-positive	173:191	arg1	endospore-forming					202:218	endospore-forming	202:218	endospore-forming	202:218	Two Gram-stain-positive, motile, endospore-forming, aerobic strains, designated V44-8T and V47-23aT, were isolated from environmental air sampling at the vehicle assembly building at Cape Canaveral, Florida, where the Viking spacecraft were assembled.
31622233	3	9	dep	Gram-stain-positive	173:191	arg1	motile					194:199	motile	194:199	motile	194:199	Two Gram-stain-positive, motile, endospore-forming, aerobic strains, designated V44-8T and V47-23aT, were isolated from environmental air sampling at the vehicle assembly building at Cape Canaveral, Florida, where the Viking spacecraft were assembled.
31622233	3	9	dep	Gram-stain-positive	173:191	arg1	aerobic					221:227	aerobic	221:227	aerobic	221:227	Two Gram-stain-positive, motile, endospore-forming, aerobic strains, designated V44-8T and V47-23aT, were isolated from environmental air sampling at the vehicle assembly building at Cape Canaveral, Florida, where the Viking spacecraft were assembled.
31622233	10	10	theme	16S	1055:1057	arg1	similarity					1073:1082	its highest 16S rRNA sequence similarity	1043:1082	its highest 16S rRNA sequence similarity with Bacillus cavernae DSM-105484T at 96.9%	1043:1126	Strain V47.23aT shared its highest 16S rRNA sequence similarity with Bacillus cavernae DSM-105484T at 96.9%, and V44.8T with Bacillus zeae DSM-103964T at 96.6 %.
31622233	14	11	theme	Bacillus	1856:1863	arg1	sp					1873:1874	Bacillus saganii sp	1856:1874	Bacillus saganii sp	1856:1874	nov. [type strain, V44-8T (=ATCC BAA-2860T =DSM 105192T)], and Bacillus saganii sp.
31622233	11	12	theme	separate	1380:1387	arg1	clade					1389:1393	a separate clade	1378:1393	a separate clade	1378:1393	Based on their phenotypic characteristics and phylogenetic position inferred from 16S rRNA gene sequence analyses, the isolates were identified as being a members of the genus Bacillus that forms a separate clade when compared to close relatives.
31622233	10	13	theme	cavernae	1098:1105	arg1	DSM-105484T					1107:1117	Bacillus cavernae DSM-105484T	1089:1117	Bacillus cavernae DSM-105484T	1089:1117	Strain V47.23aT shared its highest 16S rRNA sequence similarity with Bacillus cavernae DSM-105484T at 96.9%, and V44.8T with Bacillus zeae DSM-103964T at 96.6 %.
31622233	5	14	from	%	591:591	arg1	optimum					578:584	an optimum	575:584	an optimum at 1 % for strain V44-8T; 0 % for strain V47-23aT	575:634	Both strains displayed growth in 0-5 % NaCl with an optimum at 1 % for strain V44-8T; 0 % for strain V47-23aT.
31622233	11	15	theme	rRNA	1268:1271	arg1	analyses					1287:1294	rRNA gene sequence analyses	1268:1294	rRNA gene sequence analyses	1268:1294	Based on their phenotypic characteristics and phylogenetic position inferred from 16S rRNA gene sequence analyses, the isolates were identified as being a members of the genus Bacillus that forms a separate clade when compared to close relatives.
31622233	7	16	theme	strains	759:765	arg1	wall					746:749	The cell wall	737:749	The cell wall of both strains	737:765	The cell wall of both strains contained meso-diaminopimelic acid as the diagnostic diamino acid.
31622233	11	17	theme	phylogenetic	1228:1239	arg1	position					1241:1248	phylogenetic position	1228:1248	phylogenetic position	1228:1248	Based on their phenotypic characteristics and phylogenetic position inferred from 16S rRNA gene sequence analyses, the isolates were identified as being a members of the genus Bacillus that forms a separate clade when compared to close relatives.
31622233	9	18	theme	predominant	936:946	arg1	anteiso-C15 					974:985	anteiso-C15 	974:985	anteiso-C15 	974:985	The predominant cellular fatty acids were anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0.
31622233	9	18	theme	predominant	936:946	arg1	acids					963:967	The predominant cellular fatty acids	932:967	The predominant cellular fatty acids	932:967	The predominant cellular fatty acids were anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0.
31622233	3	19	theme	vehicle	323:329	arg1	building					340:347	the vehicle assembly building	319:347	the vehicle assembly building	319:347	Two Gram-stain-positive, motile, endospore-forming, aerobic strains, designated V44-8T and V47-23aT, were isolated from environmental air sampling at the vehicle assembly building at Cape Canaveral, Florida, where the Viking spacecraft were assembled.
31622233	13	20	theme	Bacillus	1772:1779	arg1	sp					1789:1790	Bacillus glennii sp	1772:1790	Bacillus glennii sp	1772:1790	Based on the phenotypic, genomic and biochemical data, strains V44-8T and V47-23aT represent two novel species in the genus Bacillus for which the names Bacillus glennii sp.
31622233	13	21	theme	biochemical	1656:1666	arg1	data					1668:1671	the phenotypic, genomic and biochemical data	1628:1671	the phenotypic, genomic and biochemical data	1628:1671	Based on the phenotypic, genomic and biochemical data, strains V44-8T and V47-23aT represent two novel species in the genus Bacillus for which the names Bacillus glennii sp.
31622233	13	22	theme	glennii	1781:1787	arg1	sp					1789:1790	Bacillus glennii sp	1772:1790	Bacillus glennii sp	1772:1790	Based on the phenotypic, genomic and biochemical data, strains V44-8T and V47-23aT represent two novel species in the genus Bacillus for which the names Bacillus glennii sp.
31622233	2	23	attach	isolated	57:64	arg1	assembly					83:90	the vehicle assembly	71:90	the vehicle assembly	71:90	nov., isolated from the vehicle assembly building at Kennedy Space Center where the Viking spacecraft were assembled.
31622233	2	23	attach	isolated	57:64	arg2	nov.					51:54	nov.	51:54	nov.	51:54	nov., isolated from the vehicle assembly building at Kennedy Space Center where the Viking spacecraft were assembled.
31622233	9	24	theme	cellular	948:955	arg1	anteiso-C15 					974:985	anteiso-C15 	974:985	anteiso-C15 	974:985	The predominant cellular fatty acids were anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0.
31622233	9	24	theme	cellular	948:955	arg1	acids					963:967	The predominant cellular fatty acids	932:967	The predominant cellular fatty acids	932:967	The predominant cellular fatty acids were anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0.
31622233	3	25	theme	assembly	331:338	arg1	building					340:347	the vehicle assembly building	319:347	the vehicle assembly building	319:347	Two Gram-stain-positive, motile, endospore-forming, aerobic strains, designated V44-8T and V47-23aT, were isolated from environmental air sampling at the vehicle assembly building at Cape Canaveral, Florida, where the Viking spacecraft were assembled.
31622233	13	26	dep	sp	1789:1790	arg1	names					1766:1770	the names	1762:1770	the names	1762:1770	Based on the phenotypic, genomic and biochemical data, strains V44-8T and V47-23aT represent two novel species in the genus Bacillus for which the names Bacillus glennii sp.
31622233	14	27	theme	=ATCC	1820:1824	arg1	105192T					1841:1847	=ATCC BAA-2860T =DSM 105192T	1820:1847	=ATCC BAA-2860T =DSM 105192T	1820:1847	nov. [type strain, V44-8T (=ATCC BAA-2860T =DSM 105192T)], and Bacillus saganii sp.
31622233	14	27	theme	=ATCC	1820:1824	arg1	V44-8T					1812:1817	V44-8T	1812:1817	V44-8T (=ATCC BAA-2860T =DSM 105192T)]	1812:1849	nov. [type strain, V44-8T (=ATCC BAA-2860T =DSM 105192T)], and Bacillus saganii sp.
31622233	3	28	theme	Gram-stain-positive	173:191	arg1	strains					229:235	Two Gram-stain-positive, motile, endospore-forming, aerobic strains	169:235	Two Gram-stain-positive, motile, endospore-forming, aerobic strains	169:235	Two Gram-stain-positive, motile, endospore-forming, aerobic strains, designated V44-8T and V47-23aT, were isolated from environmental air sampling at the vehicle assembly building at Cape Canaveral, Florida, where the Viking spacecraft were assembled.
31622233	9	29	theme	fatty	957:961	arg1	anteiso-C15 					974:985	anteiso-C15 	974:985	anteiso-C15 	974:985	The predominant cellular fatty acids were anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0.
31622233	9	29	theme	fatty	957:961	arg1	acids					963:967	The predominant cellular fatty acids	932:967	The predominant cellular fatty acids	932:967	The predominant cellular fatty acids were anteiso-C15 : 0, iso-C14 : 0 and iso-C15 : 0.
31622233	0	30	theme	glennii	9:15	arg1	sp					17:18	Bacillus glennii sp	0:18	Bacillus glennii sp.	0:19	Bacillus glennii sp.
31622233	2	31	theme	Space	112:116	arg1	Center					118:123	Kennedy Space Center	104:123	Kennedy Space Center where the Viking spacecraft were assembled	104:166	nov., isolated from the vehicle assembly building at Kennedy Space Center where the Viking spacecraft were assembled.
31622233	13	32	theme	genomic	1644:1650	arg1	data					1668:1671	the phenotypic, genomic and biochemical data	1628:1671	the phenotypic, genomic and biochemical data	1628:1671	Based on the phenotypic, genomic and biochemical data, strains V44-8T and V47-23aT represent two novel species in the genus Bacillus for which the names Bacillus glennii sp.
31622233	5	33	from	growth	549:554	arg1	 NaCl					564:568	0-5 % NaCl	559:568	0-5 % NaCl	559:568	Both strains displayed growth in 0-5 % NaCl with an optimum at 1 % for strain V44-8T; 0 % for strain V47-23aT.
31622233	11	34	theme	sequence	1278:1285	arg1	analyses					1287:1294	rRNA gene sequence analyses	1268:1294	rRNA gene sequence analyses	1268:1294	Based on their phenotypic characteristics and phylogenetic position inferred from 16S rRNA gene sequence analyses, the isolates were identified as being a members of the genus Bacillus that forms a separate clade when compared to close relatives.
31622233	4	35	located	observed	432:439	arg1	pH					490:491	pH 5-10	490:496	pH 5-10 (pH 9) for strain V47-23aT	490:523	Growth was observed at pH 7-9 (optimum, pH 9) for strain V44-8T, and pH 5-10 (pH 9) for strain V47-23aT.
31622233	4	35	located	observed	432:439	arg1	pH					444:445	pH 7-9	444:449	pH 7-9 (optimum, pH 9) for strain V44-8T	444:483	Growth was observed at pH 7-9 (optimum, pH 9) for strain V44-8T, and pH 5-10 (pH 9) for strain V47-23aT.
31622233	4	35	located	observed	432:439	arg1	pH					499:500	pH 9	499:502	pH 9	499:502	Growth was observed at pH 7-9 (optimum, pH 9) for strain V44-8T, and pH 5-10 (pH 9) for strain V47-23aT.
31622233	4	35	located	observed	432:439	arg2	Growth					421:426	Growth	421:426	Growth	421:426	Growth was observed at pH 7-9 (optimum, pH 9) for strain V44-8T, and pH 5-10 (pH 9) for strain V47-23aT.
31622233	4	35	located	observed	432:439	arg1	optimum					452:458	optimum	452:458	optimum	452:458	Growth was observed at pH 7-9 (optimum, pH 9) for strain V44-8T, and pH 5-10 (pH 9) for strain V47-23aT.
31622233	0	36	theme	Bacillus	0:7	arg1	sp					17:18	Bacillus glennii sp	0:18	Bacillus glennii sp.	0:19	Bacillus glennii sp.
31622233	4	37	dep	optimum	452:458	arg1	pH					461:462	pH 9	461:464	pH 9	461:464	Growth was observed at pH 7-9 (optimum, pH 9) for strain V44-8T, and pH 5-10 (pH 9) for strain V47-23aT.
31622233	14	38	theme	saganii	1865:1871	arg1	sp					1873:1874	Bacillus saganii sp	1856:1874	Bacillus saganii sp	1856:1874	nov. [type strain, V44-8T (=ATCC BAA-2860T =DSM 105192T)], and Bacillus saganii sp.
31622233	7	39	theme	cell	741:744	arg1	wall					746:749	The cell wall	737:749	The cell wall of both strains	737:765	The cell wall of both strains contained meso-diaminopimelic acid as the diagnostic diamino acid.
31622233	12	40	theme	acid	1475:1478	arg1	identity					1480:1487	average amino acid identity	1461:1487	average amino acid identity	1461:1487	Average nucleotide identity and average amino acid identity values between strains V44-8T and DSM-103964T were 72.1% and 67.5 %; V47-23aT and DSM-105484T were 62.4% and 69.1%, respectively.
31622233	10	41	from	%	1179:1179	arg1	DSM-103964T					1159:1169	Bacillus zeae DSM-103964T	1145:1169	Bacillus zeae DSM-103964T at 96.6 %	1145:1179	Strain V47.23aT shared its highest 16S rRNA sequence similarity with Bacillus cavernae DSM-105484T at 96.9%, and V44.8T with Bacillus zeae DSM-103964T at 96.6 %.
31622233	15	42	theme	BAA-2861T=DSM	1899:1911	arg1	[V47-23aT					1882:1890	nov. [V47-23aT	1877:1890	nov. [V47-23aT (=ATCC BAA-2861T=DSM 105190T)]	1877:1921	nov. [V47-23aT (=ATCC BAA-2861T=DSM 105190T)] are proposed.
31622233	15	42	theme	BAA-2861T=DSM	1899:1911	arg1	105190T					1913:1919	=ATCC BAA-2861T=DSM 105190T	1893:1919	=ATCC BAA-2861T=DSM 105190T	1893:1919	nov. [V47-23aT (=ATCC BAA-2861T=DSM 105190T)] are proposed.
31622233	12	43	theme	nucleotide	1437:1446	arg1	identity					1448:1455	Average nucleotide identity	1429:1455	Average nucleotide identity	1429:1455	Average nucleotide identity and average amino acid identity values between strains V44-8T and DSM-103964T were 72.1% and 67.5 %; V47-23aT and DSM-105484T were 62.4% and 69.1%, respectively.
31622233	10	44	with	similarity	1073:1082	arg1	DSM-105484T					1107:1117	Bacillus cavernae DSM-105484T	1089:1117	Bacillus cavernae DSM-105484T	1089:1117	Strain V47.23aT shared its highest 16S rRNA sequence similarity with Bacillus cavernae DSM-105484T at 96.9%, and V44.8T with Bacillus zeae DSM-103964T at 96.6 %.
31622233	10	45	theme	zeae	1154:1157	arg1	DSM-103964T					1159:1169	Bacillus zeae DSM-103964T	1145:1169	Bacillus zeae DSM-103964T at 96.6 %	1145:1179	Strain V47.23aT shared its highest 16S rRNA sequence similarity with Bacillus cavernae DSM-105484T at 96.9%, and V44.8T with Bacillus zeae DSM-103964T at 96.6 %.
31622233	3	46	attach	isolated	275:282	arg2	strains					229:235	Two Gram-stain-positive, motile, endospore-forming, aerobic strains	169:235	Two Gram-stain-positive, motile, endospore-forming, aerobic strains	169:235	Two Gram-stain-positive, motile, endospore-forming, aerobic strains, designated V44-8T and V47-23aT, were isolated from environmental air sampling at the vehicle assembly building at Cape Canaveral, Florida, where the Viking spacecraft were assembled.
31622233	3	46	attach	isolated	275:282	arg1	air					303:305	environmental air	289:305	environmental air sampling at the vehicle assembly building at Cape Canaveral, Florida, where the Viking spacecraft were assembled	289:418	Two Gram-stain-positive, motile, endospore-forming, aerobic strains, designated V44-8T and V47-23aT, were isolated from environmental air sampling at the vehicle assembly building at Cape Canaveral, Florida, where the Viking spacecraft were assembled.
31622233	11	47	theme	gene	1273:1276	arg1	analyses					1287:1294	rRNA gene sequence analyses	1268:1294	rRNA gene sequence analyses	1268:1294	Based on their phenotypic characteristics and phylogenetic position inferred from 16S rRNA gene sequence analyses, the isolates were identified as being a members of the genus Bacillus that forms a separate clade when compared to close relatives.
31622233	10	48	theme	Bacillus	1145:1152	arg1	DSM-103964T					1159:1169	Bacillus zeae DSM-103964T	1145:1169	Bacillus zeae DSM-103964T at 96.6 %	1145:1179	Strain V47.23aT shared its highest 16S rRNA sequence similarity with Bacillus cavernae DSM-105484T at 96.9%, and V44.8T with Bacillus zeae DSM-103964T at 96.6 %.
31622233	7	49	contain	contained	767:775	arg2	acid					797:800	meso-diaminopimelic acid	777:800	meso-diaminopimelic acid	777:800	The cell wall of both strains contained meso-diaminopimelic acid as the diagnostic diamino acid.
31622233	7	49	contain	contained	767:775	arg1	wall					746:749	The cell wall	737:749	The cell wall of both strains	737:765	The cell wall of both strains contained meso-diaminopimelic acid as the diagnostic diamino acid.
31622233	7	49	contain	contained	767:775	arg2	acid					828:831	the diagnostic diamino acid	805:831	the diagnostic diamino acid	805:831	The cell wall of both strains contained meso-diaminopimelic acid as the diagnostic diamino acid.
31622233	5	50	theme	strain	597:602	arg1	V44-8T					604:609	strain V44-8T	597:609	strain V44-8T	597:609	Both strains displayed growth in 0-5 % NaCl with an optimum at 1 % for strain V44-8T; 0 % for strain V47-23aT.
31622233	6	51	dep	Strains	637:643	arg1	V47-23aT					656:663	V47-23aT	656:663	V47-23aT	656:663	Strains V44-8T and V47-23aT grew optimally at 32 °C, (15-32 °C) and 25 °C (20-45 °C), respectively.
31622233	6	51	dep	Strains	637:643	arg1	V44-8T					645:650	V44-8T	645:650	V44-8T	645:650	Strains V44-8T and V47-23aT grew optimally at 32 °C, (15-32 °C) and 25 °C (20-45 °C), respectively.
31622233	6	51	dep	Strains	637:643	arg1	Strains					637:643	Strains	637:643	Strains V44-8T and V47-23aT	637:663	Strains V44-8T and V47-23aT grew optimally at 32 °C, (15-32 °C) and 25 °C (20-45 °C), respectively.
31622233	13	52	theme	novel	1716:1720	arg1	species					1722:1728	two novel species	1712:1728	two novel species in the genus Bacillus for which the names Bacillus glennii sp	1712:1790	Based on the phenotypic, genomic and biochemical data, strains V44-8T and V47-23aT represent two novel species in the genus Bacillus for which the names Bacillus glennii sp.
31622233	12	53	theme	identity	1480:1487	arg1	values					1489:1494	Average nucleotide identity and average amino acid identity values	1429:1494	Average nucleotide identity and average amino acid identity values between strains V44-8T and DSM-103964T	1429:1533	Average nucleotide identity and average amino acid identity values between strains V44-8T and DSM-103964T were 72.1% and 67.5 %; V47-23aT and DSM-105484T were 62.4% and 69.1%, respectively.
31622233	8	54	contain	contained	847:855	arg2	diphosphatidylglycerol					908:929	diphosphatidylglycerol	908:929	diphosphatidylglycerol	908:929	Both strains contained phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol.
31622233	8	54	contain	contained	847:855	arg2	phosphatidylethanolamine					879:902	phosphatidylethanolamine	879:902	phosphatidylethanolamine	879:902	Both strains contained phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol.
31622233	8	54	contain	contained	847:855	arg2	phosphatidylglycerol					857:876	phosphatidylglycerol	857:876	phosphatidylglycerol	857:876	Both strains contained phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol.
31622233	8	54	contain	contained	847:855	arg1	strains					839:845	Both strains	834:845	Both strains	834:845	Both strains contained phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol.
31622233	3	55	theme	Viking	387:392	arg1	spacecraft					394:403	the Viking spacecraft	383:403	the Viking spacecraft	383:403	Two Gram-stain-positive, motile, endospore-forming, aerobic strains, designated V44-8T and V47-23aT, were isolated from environmental air sampling at the vehicle assembly building at Cape Canaveral, Florida, where the Viking spacecraft were assembled.
31622233	2	56	theme	vehicle	75:81	arg1	assembly					83:90	the vehicle assembly	71:90	the vehicle assembly	71:90	nov., isolated from the vehicle assembly building at Kennedy Space Center where the Viking spacecraft were assembled.
31622233	11	57	theme	phenotypic	1197:1206	arg1	characteristics					1208:1222	their phenotypic characteristics	1191:1222	their phenotypic characteristics	1191:1222	Based on their phenotypic characteristics and phylogenetic position inferred from 16S rRNA gene sequence analyses, the isolates were identified as being a members of the genus Bacillus that forms a separate clade when compared to close relatives.
31622233	10	58	from	%	1126:1126	arg1	similarity					1073:1082	its highest 16S rRNA sequence similarity	1043:1082	its highest 16S rRNA sequence similarity with Bacillus cavernae DSM-105484T at 96.9%	1043:1126	Strain V47.23aT shared its highest 16S rRNA sequence similarity with Bacillus cavernae DSM-105484T at 96.9%, and V44.8T with Bacillus zeae DSM-103964T at 96.6 %.
31622233	13	59	dep	strains	1674:1680	arg1	strains					1674:1680	strains V44-8T and V47-23aT	1674:1700	strains V44-8T and V47-23aT	1674:1700	Based on the phenotypic, genomic and biochemical data, strains V44-8T and V47-23aT represent two novel species in the genus Bacillus for which the names Bacillus glennii sp.
31622233	13	59	dep	strains	1674:1680	arg1	V47-23aT					1693:1700	V47-23aT	1693:1700	V47-23aT	1693:1700	Based on the phenotypic, genomic and biochemical data, strains V44-8T and V47-23aT represent two novel species in the genus Bacillus for which the names Bacillus glennii sp.
31622233	13	59	dep	strains	1674:1680	arg1	V44-8T					1682:1687	V44-8T	1682:1687	V44-8T	1682:1687	Based on the phenotypic, genomic and biochemical data, strains V44-8T and V47-23aT represent two novel species in the genus Bacillus for which the names Bacillus glennii sp.
31622233	4	60	theme	strain	471:476	arg1	V44-8T					478:483	strain V44-8T	471:483	strain V44-8T	471:483	Growth was observed at pH 7-9 (optimum, pH 9) for strain V44-8T, and pH 5-10 (pH 9) for strain V47-23aT.
31622233	2	61	theme	Viking	135:140	arg1	spacecraft					142:151	the Viking spacecraft	131:151	the Viking spacecraft	131:151	nov., isolated from the vehicle assembly building at Kennedy Space Center where the Viking spacecraft were assembled.
31622233	15	62	theme	nov.	1877:1880	arg1	[V47-23aT					1882:1890	nov. [V47-23aT	1877:1890	nov. [V47-23aT (=ATCC BAA-2861T=DSM 105190T)]	1877:1921	nov. [V47-23aT (=ATCC BAA-2861T=DSM 105190T)] are proposed.
31622233	15	62	theme	nov.	1877:1880	arg1	105190T					1913:1919	=ATCC BAA-2861T=DSM 105190T	1893:1919	=ATCC BAA-2861T=DSM 105190T	1893:1919	nov. [V47-23aT (=ATCC BAA-2861T=DSM 105190T)] are proposed.
31622233	12	63	theme	Average	1429:1435	arg1	identity					1448:1455	Average nucleotide identity	1429:1455	Average nucleotide identity	1429:1455	Average nucleotide identity and average amino acid identity values between strains V44-8T and DSM-103964T were 72.1% and 67.5 %; V47-23aT and DSM-105484T were 62.4% and 69.1%, respectively.
31622233	3	64	theme	environmental	289:301	arg1	air					303:305	environmental air	289:305	environmental air sampling at the vehicle assembly building at Cape Canaveral, Florida, where the Viking spacecraft were assembled	289:418	Two Gram-stain-positive, motile, endospore-forming, aerobic strains, designated V44-8T and V47-23aT, were isolated from environmental air sampling at the vehicle assembly building at Cape Canaveral, Florida, where the Viking spacecraft were assembled.
31622233	11	65	theme	close	1412:1416	arg1	relatives					1418:1426	close relatives	1412:1426	close relatives	1412:1426	Based on their phenotypic characteristics and phylogenetic position inferred from 16S rRNA gene sequence analyses, the isolates were identified as being a members of the genus Bacillus that forms a separate clade when compared to close relatives.
31622233	10	66	theme	Bacillus	1089:1096	arg1	DSM-105484T					1107:1117	Bacillus cavernae DSM-105484T	1089:1117	Bacillus cavernae DSM-105484T	1089:1117	Strain V47.23aT shared its highest 16S rRNA sequence similarity with Bacillus cavernae DSM-105484T at 96.9%, and V44.8T with Bacillus zeae DSM-103964T at 96.6 %.
31622233	14	67	theme	BAA-2860T	1826:1834	arg1	105192T					1841:1847	=ATCC BAA-2860T =DSM 105192T	1820:1847	=ATCC BAA-2860T =DSM 105192T	1820:1847	nov. [type strain, V44-8T (=ATCC BAA-2860T =DSM 105192T)], and Bacillus saganii sp.
31622233	14	67	theme	BAA-2860T	1826:1834	arg1	V44-8T					1812:1817	V44-8T	1812:1817	V44-8T (=ATCC BAA-2860T =DSM 105192T)]	1812:1849	nov. [type strain, V44-8T (=ATCC BAA-2860T =DSM 105192T)], and Bacillus saganii sp.
31622233	13	68	from	species	1722:1728	arg1	Bacillus					1743:1750	the genus Bacillus	1733:1750	the genus Bacillus	1733:1750	Based on the phenotypic, genomic and biochemical data, strains V44-8T and V47-23aT represent two novel species in the genus Bacillus for which the names Bacillus glennii sp.
31622233	15	69	theme	=ATCC	1893:1897	arg1	[V47-23aT					1882:1890	nov. [V47-23aT	1877:1890	nov. [V47-23aT (=ATCC BAA-2861T=DSM 105190T)]	1877:1921	nov. [V47-23aT (=ATCC BAA-2861T=DSM 105190T)] are proposed.
31622233	15	69	theme	=ATCC	1893:1897	arg1	105190T					1913:1919	=ATCC BAA-2861T=DSM 105190T	1893:1919	=ATCC BAA-2861T=DSM 105190T	1893:1919	nov. [V47-23aT (=ATCC BAA-2861T=DSM 105190T)] are proposed.
31622233	14	70	theme	=DSM	1836:1839	arg1	105192T					1841:1847	=ATCC BAA-2860T =DSM 105192T	1820:1847	=ATCC BAA-2860T =DSM 105192T	1820:1847	nov. [type strain, V44-8T (=ATCC BAA-2860T =DSM 105192T)], and Bacillus saganii sp.
31622233	14	70	theme	=DSM	1836:1839	arg1	V44-8T					1812:1817	V44-8T	1812:1817	V44-8T (=ATCC BAA-2860T =DSM 105192T)]	1812:1849	nov. [type strain, V44-8T (=ATCC BAA-2860T =DSM 105192T)], and Bacillus saganii sp.
31622233	12	71	theme	identity	1448:1455	arg1	values					1489:1494	Average nucleotide identity and average amino acid identity values	1429:1494	Average nucleotide identity and average amino acid identity values between strains V44-8T and DSM-103964T	1429:1533	Average nucleotide identity and average amino acid identity values between strains V44-8T and DSM-103964T were 72.1% and 67.5 %; V47-23aT and DSM-105484T were 62.4% and 69.1%, respectively.
31622233	7	72	theme	diagnostic	809:818	arg1	acid					828:831	the diagnostic diamino acid	805:831	the diagnostic diamino acid	805:831	The cell wall of both strains contained meso-diaminopimelic acid as the diagnostic diamino acid.
31622233	7	72	theme	diagnostic	809:818	arg1	acid					797:800	meso-diaminopimelic acid	777:800	meso-diaminopimelic acid	777:800	The cell wall of both strains contained meso-diaminopimelic acid as the diagnostic diamino acid.
31622233	12	73	theme	amino	1469:1473	arg1	identity					1480:1487	average amino acid identity	1461:1487	average amino acid identity	1461:1487	Average nucleotide identity and average amino acid identity values between strains V44-8T and DSM-103964T were 72.1% and 67.5 %; V47-23aT and DSM-105484T were 62.4% and 69.1%, respectively.
31622233	4	74	theme	strain	509:514	arg1	V47-23aT					516:523	strain V47-23aT	509:523	strain V47-23aT	509:523	Growth was observed at pH 7-9 (optimum, pH 9) for strain V44-8T, and pH 5-10 (pH 9) for strain V47-23aT.
31622233	5	75	theme	strain	620:625	arg1	V47-23aT					627:634	strain V47-23aT	620:634	strain V47-23aT	620:634	Both strains displayed growth in 0-5 % NaCl with an optimum at 1 % for strain V44-8T; 0 % for strain V47-23aT.
31622233	1	76	theme	Bacillus	30:37	arg1	sp					47:48	Bacillus saganii sp	30:48	Bacillus saganii sp	30:48	nov. and Bacillus saganii sp.
31622233	7	77	theme	diamino	820:826	arg1	acid					828:831	the diagnostic diamino acid	805:831	the diagnostic diamino acid	805:831	The cell wall of both strains contained meso-diaminopimelic acid as the diagnostic diamino acid.
31622233	7	77	theme	diamino	820:826	arg1	acid					797:800	meso-diaminopimelic acid	777:800	meso-diaminopimelic acid	777:800	The cell wall of both strains contained meso-diaminopimelic acid as the diagnostic diamino acid.
31622233	10	78	theme	sequence	1064:1071	arg1	similarity					1073:1082	its highest 16S rRNA sequence similarity	1043:1082	its highest 16S rRNA sequence similarity with Bacillus cavernae DSM-105484T at 96.9%	1043:1126	Strain V47.23aT shared its highest 16S rRNA sequence similarity with Bacillus cavernae DSM-105484T at 96.9%, and V44.8T with Bacillus zeae DSM-103964T at 96.6 %.
31622233	10	79	theme	Strain	1020:1025	arg1	V47.23aT					1027:1034	Strain V47.23aT	1020:1034	Strain V47.23aT	1020:1034	Strain V47.23aT shared its highest 16S rRNA sequence similarity with Bacillus cavernae DSM-105484T at 96.9%, and V44.8T with Bacillus zeae DSM-103964T at 96.6 %.
31622233	10	80	theme	highest	1047:1053	arg1	similarity					1073:1082	its highest 16S rRNA sequence similarity	1043:1082	its highest 16S rRNA sequence similarity with Bacillus cavernae DSM-105484T at 96.9%	1043:1126	Strain V47.23aT shared its highest 16S rRNA sequence similarity with Bacillus cavernae DSM-105484T at 96.9%, and V44.8T with Bacillus zeae DSM-103964T at 96.6 %.
31622233	2	81	theme	Kennedy	104:110	arg1	Center					118:123	Kennedy Space Center	104:123	Kennedy Space Center where the Viking spacecraft were assembled	104:166	nov., isolated from the vehicle assembly building at Kennedy Space Center where the Viking spacecraft were assembled.
31622233	1	82	theme	saganii	39:45	arg1	sp					47:48	Bacillus saganii sp	30:48	Bacillus saganii sp	30:48	nov. and Bacillus saganii sp.
31622233	12	83	dep	strains	1504:1510	arg1	strains					1504:1510	strains V44-8T and DSM-103964T	1504:1533	strains V44-8T and DSM-103964T	1504:1533	Average nucleotide identity and average amino acid identity values between strains V44-8T and DSM-103964T were 72.1% and 67.5 %; V47-23aT and DSM-105484T were 62.4% and 69.1%, respectively.
31622233	12	83	dep	strains	1504:1510	arg1	DSM-103964T					1523:1533	DSM-103964T	1523:1533	DSM-103964T	1523:1533	Average nucleotide identity and average amino acid identity values between strains V44-8T and DSM-103964T were 72.1% and 67.5 %; V47-23aT and DSM-105484T were 62.4% and 69.1%, respectively.
31622233	12	83	dep	strains	1504:1510	arg1	V44-8T					1512:1517	V44-8T	1512:1517	V44-8T	1512:1517	Average nucleotide identity and average amino acid identity values between strains V44-8T and DSM-103964T were 72.1% and 67.5 %; V47-23aT and DSM-105484T were 62.4% and 69.1%, respectively.
33940221	0	0	theme	Fatty	78:82	arg1	Acids					84:88	Short-Chain Fatty Acids	66:88	Short-Chain Fatty Acids	66:88	Inulin Fermentable Fiber Ameliorates Type I Diabetes via IL22 and Short-Chain Fatty Acids in Experimental Models.
33940221	6	1	theme	depots	957:962	arg1	loss					941:944	loss	941:944	loss of adipose depots	941:962	Inulin's restoration of glycemic control prevented loss of adipose depots, while reducing food and water consumption.
33940221	1	2	theme	gut	147:149	arg1	microbiota					151:160	gut microbiota	147:160	gut microbiota	147:160	BACKGROUND & AIMS Nourishment of gut microbiota via consumption of fermentable fiber promotes gut health and guards against metabolic syndrome.
33940221	0	3	theme	Short-Chain	66:76	arg1	Acids					84:88	Short-Chain Fatty Acids	66:88	Short-Chain Fatty Acids	66:88	Inulin Fermentable Fiber Ameliorates Type I Diabetes via IL22 and Short-Chain Fatty Acids in Experimental Models.
33940221	8	4	theme	gut	1154:1156	arg1	composition					1169:1179	gut microbiota composition	1154:1179	gut microbiota composition	1154:1179	Such amelioration of diabetes was associated with alterations in gut microbiota composition and was eliminated by antibiotic administration.
33940221	1	5	theme	microbiota	151:160	arg1	Nourishment					132:142	BACKGROUND & AIMS Nourishment	114:142	BACKGROUND & AIMS Nourishment of gut microbiota via consumption of fermentable fiber	114:197	BACKGROUND & AIMS Nourishment of gut microbiota via consumption of fermentable fiber promotes gut health and guards against metabolic syndrome.
33940221	10	6	theme	SCFA-independent	1459:1474	arg1	restoration					1476:1486	SCFA-independent restoration	1459:1486	SCFA-independent restoration of interleukin 22, which was necessary and sufficient to ameliorate STZ-induced diabetes	1459:1575	Furthermore, inulin's microbiota-dependent anti-diabetic effect associated with SCFA-independent restoration of interleukin 22, which was necessary and sufficient to ameliorate STZ-induced diabetes.
33940221	8	7	theme	Such	1089:1092	arg1	amelioration					1094:1105	Such amelioration	1089:1105	Such amelioration of diabetes	1089:1117	Such amelioration of diabetes was associated with alterations in gut microbiota composition and was eliminated by antibiotic administration.
33940221	9	8	theme	fermentation	1256:1267	arg1	blockade					1244:1251	Pharmacologic blockade	1230:1251	Pharmacologic blockade of fermentation	1230:1267	Pharmacologic blockade of fermentation reduced inulin's beneficial impact on glycemic control, indicating a role for short-chain fatty acids (SCFA).
33940221	4	9	theme	wild-type	678:686	arg1	mice					688:691	wild-type mice	678:691	wild-type mice	678:691	Spontaneous (NOD mice) or STZ-induced (wild-type mice) diabetes was monitored.
33940221	10	10	theme	STZ-induced	1556:1566	arg1	diabetes					1568:1575	STZ-induced diabetes	1556:1575	STZ-induced diabetes	1556:1575	Furthermore, inulin's microbiota-dependent anti-diabetic effect associated with SCFA-independent restoration of interleukin 22, which was necessary and sufficient to ameliorate STZ-induced diabetes.
33940221	7	11	theme	pancreatic	1026:1035	arg1	function					1037:1044	pancreatic function	1026:1044	pancreatic function	1026:1044	Inulin normalized pancreatic function and markedly enhanced insulin sensitivity.
33940221	2	12	theme	dietary	275:281	arg1	fiber					283:287	dietary fiber	275:287	dietary fiber	275:287	In contrast, how dietary fiber impacts type 1 diabetes is less clear.
33940221	3	13	theme	spontaneous	446:456	arg1	models					483:488	the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models	409:488	the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models	409:488	METHODS To examine impact of dietary fibers on development of type 1 diabetes in the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models, mice were fed grain-based chow (GBC) or compositionally defined diets enriched with a fermentable fiber (inulin) or an insoluble fiber (cellulose).
33940221	4	14	theme	Spontaneous	639:649	arg1	diabetes					694:701	Spontaneous (NOD mice) or STZ-induced (wild-type mice) diabetes	639:701	Spontaneous (NOD mice) or STZ-induced (wild-type mice) diabetes	639:701	Spontaneous (NOD mice) or STZ-induced (wild-type mice) diabetes was monitored.
33940221	5	15	theme	low-fiber	743:751	arg1	diets					753:757	low-fiber diets	743:757	low-fiber diets	743:757	RESULTS Relative to GBC, low-fiber diets exacerbated STZ-induced diabetes, whereas diets enriched with inulin, but not cellulose, strongly protected against or treated it.
33940221	2	16	from	clear	321:325	arg1	contrast					261:268	contrast	261:268	contrast	261:268	In contrast, how dietary fiber impacts type 1 diabetes is less clear.
33940221	5	17	theme	STZ-induced	771:781	arg1	diabetes					783:790	exacerbated STZ-induced diabetes	759:790	exacerbated STZ-induced diabetes	759:790	RESULTS Relative to GBC, low-fiber diets exacerbated STZ-induced diabetes, whereas diets enriched with inulin, but not cellulose, strongly protected against or treated it.
33940221	0	18	theme	Experimental	93:104	arg1	Models					106:111	Experimental Models	93:111	Experimental Models	93:111	Inulin Fermentable Fiber Ameliorates Type I Diabetes via IL22 and Short-Chain Fatty Acids in Experimental Models.
33940221	7	19	theme	insulin	1068:1074	arg1	sensitivity					1076:1086	markedly enhanced insulin sensitivity	1050:1086	markedly enhanced insulin sensitivity	1050:1086	Inulin normalized pancreatic function and markedly enhanced insulin sensitivity.
33940221	8	20	theme	diabetes	1110:1117	arg1	amelioration					1094:1105	Such amelioration	1089:1105	Such amelioration of diabetes	1089:1117	Such amelioration of diabetes was associated with alterations in gut microbiota composition and was eliminated by antibiotic administration.
33940221	6	21	theme	water	989:993	arg1	consumption					995:1005	water consumption	989:1005	water consumption	989:1005	Inulin's restoration of glycemic control prevented loss of adipose depots, while reducing food and water consumption.
33940221	0	22	theme	Fermentable	7:17	arg1	Fiber					19:23	Inulin Fermentable Fiber	0:23	Inulin Fermentable Fiber	0:23	Inulin Fermentable Fiber Ameliorates Type I Diabetes via IL22 and Short-Chain Fatty Acids in Experimental Models.
33940221	8	23	from	alterations	1139:1149	arg1	composition					1169:1179	gut microbiota composition	1154:1179	gut microbiota composition	1154:1179	Such amelioration of diabetes was associated with alterations in gut microbiota composition and was eliminated by antibiotic administration.
33940221	10	24	theme	anti-diabetic	1422:1434	arg1	effect					1436:1441	inulin's microbiota-dependent anti-diabetic effect	1392:1441	inulin's microbiota-dependent anti-diabetic effect	1392:1441	Furthermore, inulin's microbiota-dependent anti-diabetic effect associated with SCFA-independent restoration of interleukin 22, which was necessary and sufficient to ameliorate STZ-induced diabetes.
33940221	12	25	theme	microbiota-dependent	1682:1701	arg1	increases					1703:1711	microbiota-dependent increases	1682:1711	microbiota-dependent increases in SCFA and interleukin 22 that, together, may have potential to prevent and/or treat type 1 diabetes.	1682:1814	CONCLUSIONS Fermentable fiber confers microbiota-dependent increases in SCFA and interleukin 22 that, together, may have potential to prevent and/or treat type 1 diabetes.
33940221	3	26	theme	diabetes	397:404	arg1	development					375:385	development	375:385	development of type 1 diabetes in the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models	375:488	METHODS To examine impact of dietary fibers on development of type 1 diabetes in the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models, mice were fed grain-based chow (GBC) or compositionally defined diets enriched with a fermentable fiber (inulin) or an insoluble fiber (cellulose).
33940221	0	27	theme	Inulin	0:5	arg1	Fiber					19:23	Inulin Fermentable Fiber	0:23	Inulin Fermentable Fiber	0:23	Inulin Fermentable Fiber Ameliorates Type I Diabetes via IL22 and Short-Chain Fatty Acids in Experimental Models.
33940221	3	28	from	impact	347:352	arg1	development					375:385	development	375:385	development of type 1 diabetes in the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models	375:488	METHODS To examine impact of dietary fibers on development of type 1 diabetes in the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models, mice were fed grain-based chow (GBC) or compositionally defined diets enriched with a fermentable fiber (inulin) or an insoluble fiber (cellulose).
33940221	9	29	theme	beneficial	1286:1295	arg1	impact					1297:1302	inulin's beneficial impact	1277:1302	inulin's beneficial impact	1277:1302	Pharmacologic blockade of fermentation reduced inulin's beneficial impact on glycemic control, indicating a role for short-chain fatty acids (SCFA).
33940221	9	30	theme	Pharmacologic	1230:1242	arg1	blockade					1244:1251	Pharmacologic blockade	1230:1251	Pharmacologic blockade of fermentation	1230:1267	Pharmacologic blockade of fermentation reduced inulin's beneficial impact on glycemic control, indicating a role for short-chain fatty acids (SCFA).
33940221	1	31	theme	fermentable	181:191	arg1	fiber					193:197	fermentable fiber	181:197	fermentable fiber	181:197	BACKGROUND & AIMS Nourishment of gut microbiota via consumption of fermentable fiber promotes gut health and guards against metabolic syndrome.
33940221	2	32	theme	type	297:300	arg1	diabetes					304:311	type 1 diabetes	297:311	type 1 diabetes	297:311	In contrast, how dietary fiber impacts type 1 diabetes is less clear.
33940221	10	33	theme	microbiota-dependent	1401:1420	arg1	effect					1436:1441	inulin's microbiota-dependent anti-diabetic effect	1392:1441	inulin's microbiota-dependent anti-diabetic effect	1392:1441	Furthermore, inulin's microbiota-dependent anti-diabetic effect associated with SCFA-independent restoration of interleukin 22, which was necessary and sufficient to ameliorate STZ-induced diabetes.
33940221	1	34	theme	fiber	193:197	arg1	consumption					166:176	consumption	166:176	consumption of fermentable fiber	166:197	BACKGROUND & AIMS Nourishment of gut microbiota via consumption of fermentable fiber promotes gut health and guards against metabolic syndrome.
33940221	3	35	theme	dietary	357:363	arg1	fibers					365:370	dietary fibers	357:370	dietary fibers	357:370	METHODS To examine impact of dietary fibers on development of type 1 diabetes in the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models, mice were fed grain-based chow (GBC) or compositionally defined diets enriched with a fermentable fiber (inulin) or an insoluble fiber (cellulose).
33940221	9	36	theme	glycemic	1307:1314	arg1	control					1316:1322	glycemic control	1307:1322	glycemic control	1307:1322	Pharmacologic blockade of fermentation reduced inulin's beneficial impact on glycemic control, indicating a role for short-chain fatty acids (SCFA).
33940221	3	37	theme	NOD	478:480	arg1	models					483:488	the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models	409:488	the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models	409:488	METHODS To examine impact of dietary fibers on development of type 1 diabetes in the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models, mice were fed grain-based chow (GBC) or compositionally defined diets enriched with a fermentable fiber (inulin) or an insoluble fiber (cellulose).
33940221	2	38	from	contrast	261:268	arg1	clear					321:325	clear	321:325	clear	321:325	In contrast, how dietary fiber impacts type 1 diabetes is less clear.
33940221	3	39	theme	-induced	433:440	arg1	models					483:488	the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models	409:488	the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models	409:488	METHODS To examine impact of dietary fibers on development of type 1 diabetes in the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models, mice were fed grain-based chow (GBC) or compositionally defined diets enriched with a fermentable fiber (inulin) or an insoluble fiber (cellulose).
33940221	3	40	theme	type	390:393	arg1	diabetes					397:404	type 1 diabetes	390:404	type 1 diabetes	390:404	METHODS To examine impact of dietary fibers on development of type 1 diabetes in the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models, mice were fed grain-based chow (GBC) or compositionally defined diets enriched with a fermentable fiber (inulin) or an insoluble fiber (cellulose).
33940221	3	41	theme	diabetes	468:475	arg1	models					483:488	the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models	409:488	the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models	409:488	METHODS To examine impact of dietary fibers on development of type 1 diabetes in the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models, mice were fed grain-based chow (GBC) or compositionally defined diets enriched with a fermentable fiber (inulin) or an insoluble fiber (cellulose).
33940221	4	42	dep	Spontaneous	639:649	arg1	mice					656:659	NOD mice	652:659	NOD mice	652:659	Spontaneous (NOD mice) or STZ-induced (wild-type mice) diabetes was monitored.
33940221	4	42	dep	Spontaneous	639:649	arg1	mice					688:691	wild-type mice	678:691	wild-type mice	678:691	Spontaneous (NOD mice) or STZ-induced (wild-type mice) diabetes was monitored.
33940221	6	43	theme	adipose	949:955	arg1	depots					957:962	adipose depots	949:962	adipose depots	949:962	Inulin's restoration of glycemic control prevented loss of adipose depots, while reducing food and water consumption.
33940221	12	44	theme	Fermentable	1656:1666	arg1	fiber					1668:1672	Fermentable fiber	1656:1672	Fermentable fiber	1656:1672	CONCLUSIONS Fermentable fiber confers microbiota-dependent increases in SCFA and interleukin 22 that, together, may have potential to prevent and/or treat type 1 diabetes.
33940221	1	45	theme	gut	208:210	arg1	health					212:217	gut health	208:217	gut health	208:217	BACKGROUND & AIMS Nourishment of gut microbiota via consumption of fermentable fiber promotes gut health and guards against metabolic syndrome.
33940221	0	46	theme	Type	37:40	arg1	Diabetes					44:51	Type I Diabetes	37:51	Type I Diabetes	37:51	Inulin Fermentable Fiber Ameliorates Type I Diabetes via IL22 and Short-Chain Fatty Acids in Experimental Models.
33940221	3	47	theme	fermentable	577:587	arg1	inulin					596:601	inulin	596:601	inulin	596:601	METHODS To examine impact of dietary fibers on development of type 1 diabetes in the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models, mice were fed grain-based chow (GBC) or compositionally defined diets enriched with a fermentable fiber (inulin) or an insoluble fiber (cellulose).
33940221	3	47	theme	fermentable	577:587	arg1	fiber					589:593	a fermentable fiber	575:593	a fermentable fiber (inulin)	575:602	METHODS To examine impact of dietary fibers on development of type 1 diabetes in the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models, mice were fed grain-based chow (GBC) or compositionally defined diets enriched with a fermentable fiber (inulin) or an insoluble fiber (cellulose).
33940221	3	48	theme	defined	547:553	arg1	diets					555:559	compositionally defined diets	531:559	compositionally defined diets enriched with a fermentable fiber (inulin) or an insoluble fiber (cellulose)	531:636	METHODS To examine impact of dietary fibers on development of type 1 diabetes in the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models, mice were fed grain-based chow (GBC) or compositionally defined diets enriched with a fermentable fiber (inulin) or an insoluble fiber (cellulose).
33940221	5	49	theme	exacerbated	759:769	arg1	diabetes					783:790	exacerbated STZ-induced diabetes	759:790	exacerbated STZ-induced diabetes	759:790	RESULTS Relative to GBC, low-fiber diets exacerbated STZ-induced diabetes, whereas diets enriched with inulin, but not cellulose, strongly protected against or treated it.
33940221	4	50	theme	STZ-induced	665:675	arg1	diabetes					694:701	Spontaneous (NOD mice) or STZ-induced (wild-type mice) diabetes	639:701	Spontaneous (NOD mice) or STZ-induced (wild-type mice) diabetes	639:701	Spontaneous (NOD mice) or STZ-induced (wild-type mice) diabetes was monitored.
33940221	8	51	theme	microbiota	1158:1167	arg1	composition					1169:1179	gut microbiota composition	1154:1179	gut microbiota composition	1154:1179	Such amelioration of diabetes was associated with alterations in gut microbiota composition and was eliminated by antibiotic administration.
33940221	0	52	from	IL22	57:60	arg1	Models					106:111	Experimental Models	93:111	Experimental Models	93:111	Inulin Fermentable Fiber Ameliorates Type I Diabetes via IL22 and Short-Chain Fatty Acids in Experimental Models.
33940221	12	53	dep	CONCLUSIONS	1644:1654	arg1	confers					1674:1680	confers	1674:1680	confers microbiota-dependent increases in SCFA and interleukin 22 that, together, may have potential to prevent and/or treat type 1 diabetes.	1674:1814	CONCLUSIONS Fermentable fiber confers microbiota-dependent increases in SCFA and interleukin 22 that, together, may have potential to prevent and/or treat type 1 diabetes.
33940221	3	54	theme	non-obese	458:466	arg1	models					483:488	the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models	409:488	the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models	409:488	METHODS To examine impact of dietary fibers on development of type 1 diabetes in the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models, mice were fed grain-based chow (GBC) or compositionally defined diets enriched with a fermentable fiber (inulin) or an insoluble fiber (cellulose).
33940221	7	55	theme	enhanced	1059:1066	arg1	sensitivity					1076:1086	markedly enhanced insulin sensitivity	1050:1086	markedly enhanced insulin sensitivity	1050:1086	Inulin normalized pancreatic function and markedly enhanced insulin sensitivity.
33940221	9	56	theme	short-chain	1347:1357	arg1	SCFA					1372:1375	SCFA	1372:1375	SCFA	1372:1375	Pharmacologic blockade of fermentation reduced inulin's beneficial impact on glycemic control, indicating a role for short-chain fatty acids (SCFA).
33940221	9	56	theme	short-chain	1347:1357	arg1	acids					1365:1369	short-chain fatty acids	1347:1369	short-chain fatty acids (SCFA)	1347:1376	Pharmacologic blockade of fermentation reduced inulin's beneficial impact on glycemic control, indicating a role for short-chain fatty acids (SCFA).
33940221	1	57	theme	BACKGROUND	114:123	arg1	Nourishment					132:142	BACKGROUND & AIMS Nourishment	114:142	BACKGROUND & AIMS Nourishment of gut microbiota via consumption of fermentable fiber	114:197	BACKGROUND & AIMS Nourishment of gut microbiota via consumption of fermentable fiber promotes gut health and guards against metabolic syndrome.
33940221	11	58	theme	NOD	1634:1636	arg1	mice					1638:1641	NOD mice	1634:1641	NOD mice	1634:1641	Inulin-enriched diets significantly delayed diabetes in NOD mice.
33940221	3	59	theme	insoluble	610:618	arg1	cellulose					627:635	cellulose	627:635	cellulose	627:635	METHODS To examine impact of dietary fibers on development of type 1 diabetes in the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models, mice were fed grain-based chow (GBC) or compositionally defined diets enriched with a fermentable fiber (inulin) or an insoluble fiber (cellulose).
33940221	3	59	theme	insoluble	610:618	arg1	fiber					620:624	an insoluble fiber	607:624	an insoluble fiber (cellulose)	607:636	METHODS To examine impact of dietary fibers on development of type 1 diabetes in the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models, mice were fed grain-based chow (GBC) or compositionally defined diets enriched with a fermentable fiber (inulin) or an insoluble fiber (cellulose).
33940221	11	60	theme	Inulin-enriched	1578:1592	arg1	diets					1594:1598	Inulin-enriched diets	1578:1598	Inulin-enriched diets	1578:1598	Inulin-enriched diets significantly delayed diabetes in NOD mice.
33940221	1	61	theme	&	125:125	arg1	Nourishment					132:142	BACKGROUND & AIMS Nourishment	114:142	BACKGROUND & AIMS Nourishment of gut microbiota via consumption of fermentable fiber	114:197	BACKGROUND & AIMS Nourishment of gut microbiota via consumption of fermentable fiber promotes gut health and guards against metabolic syndrome.
33940221	3	62	theme	grain-based	505:515	arg1	chow					517:520	grain-based chow	505:520	grain-based chow (GBC)	505:526	METHODS To examine impact of dietary fibers on development of type 1 diabetes in the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models, mice were fed grain-based chow (GBC) or compositionally defined diets enriched with a fermentable fiber (inulin) or an insoluble fiber (cellulose).
33940221	3	62	theme	grain-based	505:515	arg1	GBC					523:525	GBC	523:525	GBC	523:525	METHODS To examine impact of dietary fibers on development of type 1 diabetes in the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models, mice were fed grain-based chow (GBC) or compositionally defined diets enriched with a fermentable fiber (inulin) or an insoluble fiber (cellulose).
33940221	12	63	contain	have	1760:1763	arg2	potential					1765:1773	potential	1765:1773	potential	1765:1773	CONCLUSIONS Fermentable fiber confers microbiota-dependent increases in SCFA and interleukin 22 that, together, may have potential to prevent and/or treat type 1 diabetes.
33940221	12	63	contain	have	1760:1763	arg1	increases					1703:1711	microbiota-dependent increases	1682:1711	microbiota-dependent increases in SCFA and interleukin 22 that, together, may have potential to prevent and/or treat type 1 diabetes.	1682:1814	CONCLUSIONS Fermentable fiber confers microbiota-dependent increases in SCFA and interleukin 22 that, together, may have potential to prevent and/or treat type 1 diabetes.
33940221	10	64	theme	interleukin	1491:1501	arg1	restoration					1476:1486	SCFA-independent restoration	1459:1486	SCFA-independent restoration of interleukin 22, which was necessary and sufficient to ameliorate STZ-induced diabetes	1459:1575	Furthermore, inulin's microbiota-dependent anti-diabetic effect associated with SCFA-independent restoration of interleukin 22, which was necessary and sufficient to ameliorate STZ-induced diabetes.
33940221	12	65	from	increases	1703:1711	arg1	interleukin					1725:1735	interleukin 22	1725:1738	interleukin 22	1725:1738	CONCLUSIONS Fermentable fiber confers microbiota-dependent increases in SCFA and interleukin 22 that, together, may have potential to prevent and/or treat type 1 diabetes.
33940221	12	65	from	increases	1703:1711	arg1	SCFA					1716:1719	SCFA	1716:1719	SCFA	1716:1719	CONCLUSIONS Fermentable fiber confers microbiota-dependent increases in SCFA and interleukin 22 that, together, may have potential to prevent and/or treat type 1 diabetes.
33940221	9	66	theme	fatty	1359:1363	arg1	SCFA					1372:1375	SCFA	1372:1375	SCFA	1372:1375	Pharmacologic blockade of fermentation reduced inulin's beneficial impact on glycemic control, indicating a role for short-chain fatty acids (SCFA).
33940221	9	66	theme	fatty	1359:1363	arg1	acids					1365:1369	short-chain fatty acids	1347:1369	short-chain fatty acids (SCFA)	1347:1376	Pharmacologic blockade of fermentation reduced inulin's beneficial impact on glycemic control, indicating a role for short-chain fatty acids (SCFA).
33940221	1	67	theme	AIMS	127:130	arg1	Nourishment					132:142	BACKGROUND & AIMS Nourishment	114:142	BACKGROUND & AIMS Nourishment of gut microbiota via consumption of fermentable fiber	114:197	BACKGROUND & AIMS Nourishment of gut microbiota via consumption of fermentable fiber promotes gut health and guards against metabolic syndrome.
33940221	4	68	theme	NOD	652:654	arg1	mice					656:659	NOD mice	652:659	NOD mice	652:659	Spontaneous (NOD mice) or STZ-induced (wild-type mice) diabetes was monitored.
33940221	12	69	theme	type	1799:1802	arg1	diabetes					1806:1813	type 1 diabetes	1799:1813	type 1 diabetes	1799:1813	CONCLUSIONS Fermentable fiber confers microbiota-dependent increases in SCFA and interleukin 22 that, together, may have potential to prevent and/or treat type 1 diabetes.
33940221	0	70	from	Acids	84:88	arg1	Models					106:111	Experimental Models	93:111	Experimental Models	93:111	Inulin Fermentable Fiber Ameliorates Type I Diabetes via IL22 and Short-Chain Fatty Acids in Experimental Models.
33940221	8	71	theme	antibiotic	1203:1212	arg1	administration					1214:1227	antibiotic administration	1203:1227	antibiotic administration	1203:1227	Such amelioration of diabetes was associated with alterations in gut microbiota composition and was eliminated by antibiotic administration.
33940221	6	72	theme	control	923:929	arg1	restoration					899:909	Inulin's restoration	890:909	Inulin's restoration of glycemic control	890:929	Inulin's restoration of glycemic control prevented loss of adipose depots, while reducing food and water consumption.
33940221	3	73	from	development	375:385	arg1	models					483:488	the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models	409:488	the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models	409:488	METHODS To examine impact of dietary fibers on development of type 1 diabetes in the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models, mice were fed grain-based chow (GBC) or compositionally defined diets enriched with a fermentable fiber (inulin) or an insoluble fiber (cellulose).
33940221	6	74	theme	glycemic	914:921	arg1	control					923:929	glycemic control	914:929	glycemic control	914:929	Inulin's restoration of glycemic control prevented loss of adipose depots, while reducing food and water consumption.
33940221	3	75	theme	fibers	365:370	arg1	impact					347:352	impact	347:352	impact of dietary fibers on development of type 1 diabetes in the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models	347:488	METHODS To examine impact of dietary fibers on development of type 1 diabetes in the streptozotocin (STZ)-induced and spontaneous non-obese diabetes (NOD) models, mice were fed grain-based chow (GBC) or compositionally defined diets enriched with a fermentable fiber (inulin) or an insoluble fiber (cellulose).
33940221	1	76	theme	metabolic	238:246	arg1	syndrome					248:255	metabolic syndrome	238:255	metabolic syndrome	238:255	BACKGROUND & AIMS Nourishment of gut microbiota via consumption of fermentable fiber promotes gut health and guards against metabolic syndrome.
32886601	11	0	theme	type	1480:1483	arg1	H23T48T					1495:1501	H23T48T	1495:1501	H23T48T (=KCTC 49253T=JCM 33282T)	1495:1527	The type strain is H23T48T (=KCTC 49253T=JCM 33282T).
32886601	11	0	theme	type	1480:1483	arg1	strain					1485:1490	The type strain	1476:1490	The type strain	1476:1490	The type strain is H23T48T (=KCTC 49253T=JCM 33282T).
32886601	4	1	theme	Flaviflexus	621:631	arg1	EBR4-1-2T					652:660	Flaviflexus salsibiostraticola EBR4-1-2T	621:660	Flaviflexus salsibiostraticola EBR4-1-2T	621:660	16S rRNA gene sequence-based phylogenetic analysis revealed that strain H23T48T was closely related to the genus Flaviflexus, with 97.0 and 96.7 % sequence similarities to Flaviflexus salsibiostraticola EBR4-1-2T and Flaviflexus huanghaiensis H5T, respectively.
32886601	4	2	theme	strain	514:519	arg1	H23T48T					521:527	strain H23T48T	514:527	strain H23T48T	514:527	16S rRNA gene sequence-based phylogenetic analysis revealed that strain H23T48T was closely related to the genus Flaviflexus, with 97.0 and 96.7 % sequence similarities to Flaviflexus salsibiostraticola EBR4-1-2T and Flaviflexus huanghaiensis H5T, respectively.
32886601	5	3	theme	 1	797:798	arg1	ω9c					800:802	 1 ω9c	797:802	 1 ω9c (31.3 %)	797:811	Strain H23T48T possessed MK-9(H4) as the major menaquinone and C16 : 0 (42.4 %), C18 : 1 ω9c (31.3 %) and C14 : 0 (17.7 %) as the major cellular fatty acids.
32886601	5	3	theme	 1	797:798	arg1	%					810:810	31.3 %	805:810	31.3 %	805:810	Strain H23T48T possessed MK-9(H4) as the major menaquinone and C16 : 0 (42.4 %), C18 : 1 ω9c (31.3 %) and C14 : 0 (17.7 %) as the major cellular fatty acids.
32886601	9	4	theme	obtained	1331:1338	arg1	data					1340:1343	the obtained data	1327:1343	the obtained data	1327:1343	Based on the obtained data, strain H23T48T represents a novel species of the genus Flaviflexus, for which the name Flaviflexus ciconiae sp.
32886601	7	5	theme	acid	1024:1027	arg1	composition					1029:1039	The amino acid composition	1014:1039	The amino acid composition of the cell-wall peptidoglycan	1014:1070	The amino acid composition of the cell-wall peptidoglycan was l-alanine, l-lysine, d-glutamic acid, l-aspartic acid and glycine.
32886601	7	5	theme	acid	1024:1027	arg1	l-alanine					1076:1084	l-alanine	1076:1084	l-alanine	1076:1084	The amino acid composition of the cell-wall peptidoglycan was l-alanine, l-lysine, d-glutamic acid, l-aspartic acid and glycine.
32886601	4	6	with	related	541:547	arg1	H5T					692:694	H5T	692:694	H5T	692:694	16S rRNA gene sequence-based phylogenetic analysis revealed that strain H23T48T was closely related to the genus Flaviflexus, with 97.0 and 96.7 % sequence similarities to Flaviflexus salsibiostraticola EBR4-1-2T and Flaviflexus huanghaiensis H5T, respectively.
32886601	4	6	with	related	541:547	arg1	%					594:594	97.0 and 96.7 %	580:594	97.0 and 96.7 % sequence similarities to Flaviflexus salsibiostraticola EBR4-1-2T	580:660	16S rRNA gene sequence-based phylogenetic analysis revealed that strain H23T48T was closely related to the genus Flaviflexus, with 97.0 and 96.7 % sequence similarities to Flaviflexus salsibiostraticola EBR4-1-2T and Flaviflexus huanghaiensis H5T, respectively.
32886601	4	7	dep	Flaviflexus	621:631	arg1	salsibiostraticola					633:650	salsibiostraticola	633:650	salsibiostraticola	633:650	16S rRNA gene sequence-based phylogenetic analysis revealed that strain H23T48T was closely related to the genus Flaviflexus, with 97.0 and 96.7 % sequence similarities to Flaviflexus salsibiostraticola EBR4-1-2T and Flaviflexus huanghaiensis H5T, respectively.
32886601	9	8	theme	Flaviflexus	1401:1411	arg1	species					1380:1386	a novel species	1372:1386	a novel species	1372:1386	Based on the obtained data, strain H23T48T represents a novel species of the genus Flaviflexus, for which the name Flaviflexus ciconiae sp.
32886601	8	9	theme	59.5 mol	1188:1195	arg1	content					1159:1165	The genomic G+C content	1143:1165	The genomic G+C content of strain H23T48T	1143:1183	The genomic G+C content of strain H23T48T is 59.5 mol% and the average nucleotide identity value between H23T48T and F. salsibiostraticola KCT C33148T (=EBR4-1-2T) is 75.5 %.
32886601	8	9	theme	59.5 mol	1188:1195	arg1	%					1196:1196	59.5 mol%	1188:1196	59.5 mol%	1188:1196	The genomic G+C content of strain H23T48T is 59.5 mol% and the average nucleotide identity value between H23T48T and F. salsibiostraticola KCT C33148T (=EBR4-1-2T) is 75.5 %.
32886601	1	10	attach	isolated	31:38	arg2	nov.					25:28	nov.	25:28	nov.	25:28	nov., isolated from the faeces of the oriental stork, Ciconia boyciana.
32886601	1	10	attach	isolated	31:38	arg1	faeces					49:54	the faeces	45:54	the faeces of the oriental stork	45:76	nov., isolated from the faeces of the oriental stork, Ciconia boyciana.
32886601	7	11	theme	cell-wall	1048:1056	arg1	peptidoglycan					1058:1070	the cell-wall peptidoglycan	1044:1070	the cell-wall peptidoglycan	1044:1070	The amino acid composition of the cell-wall peptidoglycan was l-alanine, l-lysine, d-glutamic acid, l-aspartic acid and glycine.
32886601	6	12	theme	unidentified	981:992	arg1	glycophospholipids					994:1011	two unidentified glycophospholipids	977:1011	two unidentified glycophospholipids	977:1011	The polar lipids included phosphatidylglycerol, two unidentified lipids, six unidentified phospholipids and two unidentified glycophospholipids.
32886601	6	12	theme	unidentified	981:992	arg1	phosphatidylglycerol					895:914	phosphatidylglycerol	895:914	phosphatidylglycerol	895:914	The polar lipids included phosphatidylglycerol, two unidentified lipids, six unidentified phospholipids and two unidentified glycophospholipids.
32886601	1	13	dep	boyciana	87:94	arg1	nov.					25:28	nov.	25:28	nov.	25:28	nov., isolated from the faeces of the oriental stork, Ciconia boyciana.
32886601	8	14	dep	identity	1225:1232	arg1	value					1234:1238	value	1234:1238	the average nucleotide identity value between H23T48T and F. salsibiostraticola KCT C33148T (=EBR4-1-2T) is 75.5 %	1202:1315	The genomic G+C content of strain H23T48T is 59.5 mol% and the average nucleotide identity value between H23T48T and F. salsibiostraticola KCT C33148T (=EBR4-1-2T) is 75.5 %.
32886601	6	15	theme	unidentified	921:932	arg1	lipids					934:939	two unidentified lipids	917:939	two unidentified lipids	917:939	The polar lipids included phosphatidylglycerol, two unidentified lipids, six unidentified phospholipids and two unidentified glycophospholipids.
32886601	6	15	theme	unidentified	921:932	arg1	phosphatidylglycerol					895:914	phosphatidylglycerol	895:914	phosphatidylglycerol	895:914	The polar lipids included phosphatidylglycerol, two unidentified lipids, six unidentified phospholipids and two unidentified glycophospholipids.
32886601	7	16	theme	peptidoglycan	1058:1070	arg1	composition					1029:1039	The amino acid composition	1014:1039	The amino acid composition of the cell-wall peptidoglycan	1014:1070	The amino acid composition of the cell-wall peptidoglycan was l-alanine, l-lysine, d-glutamic acid, l-aspartic acid and glycine.
32886601	7	16	theme	peptidoglycan	1058:1070	arg1	l-alanine					1076:1084	l-alanine	1076:1084	l-alanine	1076:1084	The amino acid composition of the cell-wall peptidoglycan was l-alanine, l-lysine, d-glutamic acid, l-aspartic acid and glycine.
32886601	8	17	dep	H23T48T	1248:1254	arg1	%					1315:1315	75.5 %	1310:1315	75.5 %	1310:1315	The genomic G+C content of strain H23T48T is 59.5 mol% and the average nucleotide identity value between H23T48T and F. salsibiostraticola KCT C33148T (=EBR4-1-2T) is 75.5 %.
32886601	9	18	dep	Flaviflexus	1433:1443	arg1	ciconiae					1445:1452	ciconiae	1445:1452	ciconiae	1445:1452	Based on the obtained data, strain H23T48T represents a novel species of the genus Flaviflexus, for which the name Flaviflexus ciconiae sp.
32886601	8	19	theme	G+C	1155:1157	arg1	content					1159:1165	The genomic G+C content	1143:1165	The genomic G+C content of strain H23T48T	1143:1183	The genomic G+C content of strain H23T48T is 59.5 mol% and the average nucleotide identity value between H23T48T and F. salsibiostraticola KCT C33148T (=EBR4-1-2T) is 75.5 %.
32886601	8	19	theme	G+C	1155:1157	arg1	%					1196:1196	59.5 mol%	1188:1196	59.5 mol%	1188:1196	The genomic G+C content of strain H23T48T is 59.5 mol% and the average nucleotide identity value between H23T48T and F. salsibiostraticola KCT C33148T (=EBR4-1-2T) is 75.5 %.
32886601	2	20	theme	faecal	256:261	arg1	sample					263:268	the faecal sample	252:268	the faecal sample of an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea	252:357	A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium, designated strain H23T48T, was isolated from the faecal sample of an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea.
32886601	9	21	theme	genus	1395:1399	arg1	Flaviflexus					1401:1411	the genus Flaviflexus	1391:1411	the genus Flaviflexus	1391:1411	Based on the obtained data, strain H23T48T represents a novel species of the genus Flaviflexus, for which the name Flaviflexus ciconiae sp.
32886601	0	22	theme	ciconiae	12:19	arg1	sp					21:22	Flaviflexus ciconiae sp	0:22	Flaviflexus ciconiae sp.	0:23	Flaviflexus ciconiae sp.
32886601	2	23	theme	Gram-stain-positive	105:123	arg1	H23T48T					225:231	designated strain H23T48T	207:231	designated strain H23T48T	207:231	A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium, designated strain H23T48T, was isolated from the faecal sample of an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea.
32886601	2	23	theme	Gram-stain-positive	105:123	arg1	bacterium					196:204	A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium	97:204	A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium	97:204	A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium, designated strain H23T48T, was isolated from the faecal sample of an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea.
32886601	3	24	located	observed	397:404	arg2	growth					368:373	Optimal growth	360:373	Optimal growth of strain H23T48T	360:391	Optimal growth of strain H23T48T was observed at 30-37 °C, pH 8 and with 3 % (w/v) NaCl.
32886601	3	24	located	observed	397:404	arg1	pH					419:420	pH 8	419:422	pH 8	419:422	Optimal growth of strain H23T48T was observed at 30-37 °C, pH 8 and with 3 % (w/v) NaCl.
32886601	3	24	located	observed	397:404	arg1	30-37 °C					409:416	30-37 °C	409:416	30-37 °C	409:416	Optimal growth of strain H23T48T was observed at 30-37 °C, pH 8 and with 3 % (w/v) NaCl.
32886601	1	25	theme	oriental	63:70	arg1	stork					72:76	the oriental stork	59:76	the oriental stork	59:76	nov., isolated from the faeces of the oriental stork, Ciconia boyciana.
32886601	3	26	theme	H23T48T	385:391	arg1	growth					368:373	Optimal growth	360:373	Optimal growth of strain H23T48T	360:391	Optimal growth of strain H23T48T was observed at 30-37 °C, pH 8 and with 3 % (w/v) NaCl.
32886601	0	27	theme	Flaviflexus	0:10	arg1	sp					21:22	Flaviflexus ciconiae sp	0:22	Flaviflexus ciconiae sp.	0:23	Flaviflexus ciconiae sp.
32886601	4	28	theme	phylogenetic	478:489	arg1	analysis					491:498	16S rRNA gene sequence-based phylogenetic analysis	449:498	16S rRNA gene sequence-based phylogenetic analysis	449:498	16S rRNA gene sequence-based phylogenetic analysis revealed that strain H23T48T was closely related to the genus Flaviflexus, with 97.0 and 96.7 % sequence similarities to Flaviflexus salsibiostraticola EBR4-1-2T and Flaviflexus huanghaiensis H5T, respectively.
32886601	1	29	theme	stork	72:76	arg1	faeces					49:54	the faeces	45:54	the faeces of the oriental stork	45:76	nov., isolated from the faeces of the oriental stork, Ciconia boyciana.
32886601	8	30	theme	average	1206:1212	arg1	identity					1225:1232	the average nucleotide identity	1202:1232	the average nucleotide identity value between H23T48T and F. salsibiostraticola KCT C33148T (=EBR4-1-2T) is 75.5 %	1202:1315	The genomic G+C content of strain H23T48T is 59.5 mol% and the average nucleotide identity value between H23T48T and F. salsibiostraticola KCT C33148T (=EBR4-1-2T) is 75.5 %.
32886601	5	31	theme	major	752:756	arg1	MK-9					736:739	MK-9	736:739	MK-9(H4)	736:743	Strain H23T48T possessed MK-9(H4) as the major menaquinone and C16 : 0 (42.4 %), C18 : 1 ω9c (31.3 %) and C14 : 0 (17.7 %) as the major cellular fatty acids.
32886601	5	31	theme	major	752:756	arg1	menaquinone					758:768	the major menaquinone	748:768	the major menaquinone	748:768	Strain H23T48T possessed MK-9(H4) as the major menaquinone and C16 : 0 (42.4 %), C18 : 1 ω9c (31.3 %) and C14 : 0 (17.7 %) as the major cellular fatty acids.
32886601	2	32	dep	Gram-stain-positive	105:123	arg1	coccobacillus-shaped					157:176	coccobacillus-shaped	157:176	coccobacillus-shaped	157:176	A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium, designated strain H23T48T, was isolated from the faecal sample of an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea.
32886601	2	32	dep	Gram-stain-positive	105:123	arg1	aerobic					188:194	aerobic	188:194	aerobic	188:194	A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium, designated strain H23T48T, was isolated from the faecal sample of an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea.
32886601	2	32	dep	Gram-stain-positive	105:123	arg1	non-spore-forming					138:154	non-spore-forming	138:154	non-spore-forming	138:154	A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium, designated strain H23T48T, was isolated from the faecal sample of an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea.
32886601	2	32	dep	Gram-stain-positive	105:123	arg1	non-motile					126:135	non-motile	126:135	non-motile	126:135	A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium, designated strain H23T48T, was isolated from the faecal sample of an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea.
32886601	2	33	theme	stork	285:289	arg1	sample					263:268	the faecal sample	252:268	the faecal sample of an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea	252:357	A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium, designated strain H23T48T, was isolated from the faecal sample of an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea.
32886601	8	34	theme	nucleotide	1214:1223	arg1	identity					1225:1232	the average nucleotide identity	1202:1232	the average nucleotide identity value between H23T48T and F. salsibiostraticola KCT C33148T (=EBR4-1-2T) is 75.5 %	1202:1315	The genomic G+C content of strain H23T48T is 59.5 mol% and the average nucleotide identity value between H23T48T and F. salsibiostraticola KCT C33148T (=EBR4-1-2T) is 75.5 %.
32886601	5	35	dep	possessed	726:734	arg1	 0					822:823	 0	822:823	 0	822:823	Strain H23T48T possessed MK-9(H4) as the major menaquinone and C16 : 0 (42.4 %), C18 : 1 ω9c (31.3 %) and C14 : 0 (17.7 %) as the major cellular fatty acids.
32886601	5	35	dep	possessed	726:734	arg1	%					810:810	31.3 %	805:810	31.3 %	805:810	Strain H23T48T possessed MK-9(H4) as the major menaquinone and C16 : 0 (42.4 %), C18 : 1 ω9c (31.3 %) and C14 : 0 (17.7 %) as the major cellular fatty acids.
32886601	5	35	dep	possessed	726:734	arg1	 0					779:780	 0	779:780	 0	779:780	Strain H23T48T possessed MK-9(H4) as the major menaquinone and C16 : 0 (42.4 %), C18 : 1 ω9c (31.3 %) and C14 : 0 (17.7 %) as the major cellular fatty acids.
32886601	5	35	dep	possessed	726:734	arg1	C18 					792:795	C18 	792:795	C18 	792:795	Strain H23T48T possessed MK-9(H4) as the major menaquinone and C16 : 0 (42.4 %), C18 : 1 ω9c (31.3 %) and C14 : 0 (17.7 %) as the major cellular fatty acids.
32886601	5	35	dep	possessed	726:734	arg1	%					831:831	17.7 %	826:831	17.7 %	826:831	Strain H23T48T possessed MK-9(H4) as the major menaquinone and C16 : 0 (42.4 %), C18 : 1 ω9c (31.3 %) and C14 : 0 (17.7 %) as the major cellular fatty acids.
32886601	5	35	dep	possessed	726:734	arg1	C14 					817:820	C14 	817:820	C14 	817:820	Strain H23T48T possessed MK-9(H4) as the major menaquinone and C16 : 0 (42.4 %), C18 : 1 ω9c (31.3 %) and C14 : 0 (17.7 %) as the major cellular fatty acids.
32886601	5	35	dep	possessed	726:734	arg1	%					788:788	42.4 %	783:788	42.4 %	783:788	Strain H23T48T possessed MK-9(H4) as the major menaquinone and C16 : 0 (42.4 %), C18 : 1 ω9c (31.3 %) and C14 : 0 (17.7 %) as the major cellular fatty acids.
32886601	5	35	dep	possessed	726:734	arg1	ω9c					800:802	 1 ω9c	797:802	 1 ω9c (31.3 %)	797:811	Strain H23T48T possessed MK-9(H4) as the major menaquinone and C16 : 0 (42.4 %), C18 : 1 ω9c (31.3 %) and C14 : 0 (17.7 %) as the major cellular fatty acids.
32886601	2	36	theme	oriental	276:283	arg1	stork					285:289	an oriental stork	273:289	an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea	273:357	A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium, designated strain H23T48T, was isolated from the faecal sample of an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea.
32886601	9	37	theme	novel	1374:1378	arg1	species					1380:1386	a novel species	1372:1386	a novel species	1372:1386	Based on the obtained data, strain H23T48T represents a novel species of the genus Flaviflexus, for which the name Flaviflexus ciconiae sp.
32886601	4	38	theme	genus	556:560	arg1	Flaviflexus					562:572	the genus Flaviflexus	552:572	the genus Flaviflexus	552:572	16S rRNA gene sequence-based phylogenetic analysis revealed that strain H23T48T was closely related to the genus Flaviflexus, with 97.0 and 96.7 % sequence similarities to Flaviflexus salsibiostraticola EBR4-1-2T and Flaviflexus huanghaiensis H5T, respectively.
32886601	8	39	theme	genomic	1147:1153	arg1	content					1159:1165	The genomic G+C content	1143:1165	The genomic G+C content of strain H23T48T	1143:1183	The genomic G+C content of strain H23T48T is 59.5 mol% and the average nucleotide identity value between H23T48T and F. salsibiostraticola KCT C33148T (=EBR4-1-2T) is 75.5 %.
32886601	8	39	theme	genomic	1147:1153	arg1	%					1196:1196	59.5 mol%	1188:1196	59.5 mol%	1188:1196	The genomic G+C content of strain H23T48T is 59.5 mol% and the average nucleotide identity value between H23T48T and F. salsibiostraticola KCT C33148T (=EBR4-1-2T) is 75.5 %.
32886601	7	40	theme	l-aspartic	1114:1123	arg1	acid					1125:1128	l-aspartic acid	1114:1128	l-aspartic acid	1114:1128	The amino acid composition of the cell-wall peptidoglycan was l-alanine, l-lysine, d-glutamic acid, l-aspartic acid and glycine.
32886601	5	41	theme	cellular	847:854	arg1	acids					862:866	the major cellular fatty acids	837:866	the major cellular fatty acids	837:866	Strain H23T48T possessed MK-9(H4) as the major menaquinone and C16 : 0 (42.4 %), C18 : 1 ω9c (31.3 %) and C14 : 0 (17.7 %) as the major cellular fatty acids.
32886601	6	42	theme	polar	873:877	arg1	lipids					879:884	The polar lipids	869:884	The polar lipids	869:884	The polar lipids included phosphatidylglycerol, two unidentified lipids, six unidentified phospholipids and two unidentified glycophospholipids.
32886601	3	43	theme	strain	378:383	arg1	H23T48T					385:391	strain H23T48T	378:391	strain H23T48T	378:391	Optimal growth of strain H23T48T was observed at 30-37 °C, pH 8 and with 3 % (w/v) NaCl.
32886601	4	44	theme	16S	449:451	arg1	rRNA					453:456	16S rRNA	449:456	16S rRNA gene sequence-based phylogenetic analysis	449:498	16S rRNA gene sequence-based phylogenetic analysis revealed that strain H23T48T was closely related to the genus Flaviflexus, with 97.0 and 96.7 % sequence similarities to Flaviflexus salsibiostraticola EBR4-1-2T and Flaviflexus huanghaiensis H5T, respectively.
32886601	7	45	theme	amino	1018:1022	arg1	composition					1029:1039	The amino acid composition	1014:1039	The amino acid composition of the cell-wall peptidoglycan	1014:1070	The amino acid composition of the cell-wall peptidoglycan was l-alanine, l-lysine, d-glutamic acid, l-aspartic acid and glycine.
32886601	7	45	theme	amino	1018:1022	arg1	l-alanine					1076:1084	l-alanine	1076:1084	l-alanine	1076:1084	The amino acid composition of the cell-wall peptidoglycan was l-alanine, l-lysine, d-glutamic acid, l-aspartic acid and glycine.
32886601	11	46	theme	=KCTC	1504:1508	arg1	33282T					1521:1526	=KCTC 49253T=JCM 33282T	1504:1526	=KCTC 49253T=JCM 33282T	1504:1526	The type strain is H23T48T (=KCTC 49253T=JCM 33282T).
32886601	11	46	theme	=KCTC	1504:1508	arg1	H23T48T					1495:1501	H23T48T	1495:1501	H23T48T (=KCTC 49253T=JCM 33282T)	1495:1527	The type strain is H23T48T (=KCTC 49253T=JCM 33282T).
32886601	5	47	theme	fatty	856:860	arg1	acids					862:866	the major cellular fatty acids	837:866	the major cellular fatty acids	837:866	Strain H23T48T possessed MK-9(H4) as the major menaquinone and C16 : 0 (42.4 %), C18 : 1 ω9c (31.3 %) and C14 : 0 (17.7 %) as the major cellular fatty acids.
32886601	3	48	theme	Optimal	360:366	arg1	growth					368:373	Optimal growth	360:373	Optimal growth of strain H23T48T	360:391	Optimal growth of strain H23T48T was observed at 30-37 °C, pH 8 and with 3 % (w/v) NaCl.
32886601	4	49	theme	sequence-based	463:476	arg1	analysis					491:498	16S rRNA gene sequence-based phylogenetic analysis	449:498	16S rRNA gene sequence-based phylogenetic analysis	449:498	16S rRNA gene sequence-based phylogenetic analysis revealed that strain H23T48T was closely related to the genus Flaviflexus, with 97.0 and 96.7 % sequence similarities to Flaviflexus salsibiostraticola EBR4-1-2T and Flaviflexus huanghaiensis H5T, respectively.
32886601	2	50	theme	strain	218:223	arg1	H23T48T					225:231	designated strain H23T48T	207:231	designated strain H23T48T	207:231	A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium, designated strain H23T48T, was isolated from the faecal sample of an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea.
32886601	2	50	theme	strain	218:223	arg1	bacterium					196:204	A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium	97:204	A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium	97:204	A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium, designated strain H23T48T, was isolated from the faecal sample of an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea.
32886601	3	51	theme	3 	433:434	arg1	%					435:435	%	435:435	%	435:435	Optimal growth of strain H23T48T was observed at 30-37 °C, pH 8 and with 3 % (w/v) NaCl.
32886601	9	52	theme	strain	1346:1351	arg1	H23T48T					1353:1359	strain H23T48T	1346:1359	strain H23T48T	1346:1359	Based on the obtained data, strain H23T48T represents a novel species of the genus Flaviflexus, for which the name Flaviflexus ciconiae sp.
32886601	4	53	theme	gene	458:461	arg1	analysis					491:498	16S rRNA gene sequence-based phylogenetic analysis	449:498	16S rRNA gene sequence-based phylogenetic analysis	449:498	16S rRNA gene sequence-based phylogenetic analysis revealed that strain H23T48T was closely related to the genus Flaviflexus, with 97.0 and 96.7 % sequence similarities to Flaviflexus salsibiostraticola EBR4-1-2T and Flaviflexus huanghaiensis H5T, respectively.
32886601	5	54	contain	possessed	726:734	arg2	menaquinone					758:768	the major menaquinone	748:768	the major menaquinone	748:768	Strain H23T48T possessed MK-9(H4) as the major menaquinone and C16 : 0 (42.4 %), C18 : 1 ω9c (31.3 %) and C14 : 0 (17.7 %) as the major cellular fatty acids.
32886601	5	54	contain	possessed	726:734	arg2	MK-9					736:739	MK-9	736:739	MK-9(H4)	736:743	Strain H23T48T possessed MK-9(H4) as the major menaquinone and C16 : 0 (42.4 %), C18 : 1 ω9c (31.3 %) and C14 : 0 (17.7 %) as the major cellular fatty acids.
32886601	5	54	contain	possessed	726:734	arg1	H23T48T					718:724	Strain H23T48T	711:724	Strain H23T48T	711:724	Strain H23T48T possessed MK-9(H4) as the major menaquinone and C16 : 0 (42.4 %), C18 : 1 ω9c (31.3 %) and C14 : 0 (17.7 %) as the major cellular fatty acids.
32886601	5	54	contain	possessed	726:734	arg2	H4					741:742	H4	741:742	H4	741:742	Strain H23T48T possessed MK-9(H4) as the major menaquinone and C16 : 0 (42.4 %), C18 : 1 ω9c (31.3 %) and C14 : 0 (17.7 %) as the major cellular fatty acids.
32886601	2	55	theme	designated	207:216	arg1	H23T48T					225:231	designated strain H23T48T	207:231	designated strain H23T48T	207:231	A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium, designated strain H23T48T, was isolated from the faecal sample of an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea.
32886601	2	55	theme	designated	207:216	arg1	bacterium					196:204	A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium	97:204	A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium	97:204	A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium, designated strain H23T48T, was isolated from the faecal sample of an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea.
32886601	3	56	theme	%	435:435	arg1	NaCl					443:446	3 % (w/v) NaCl	433:446	3 % (w/v) NaCl	433:446	Optimal growth of strain H23T48T was observed at 30-37 °C, pH 8 and with 3 % (w/v) NaCl.
32886601	4	57	theme	rRNA	453:456	arg1	analysis					491:498	16S rRNA gene sequence-based phylogenetic analysis	449:498	16S rRNA gene sequence-based phylogenetic analysis	449:498	16S rRNA gene sequence-based phylogenetic analysis revealed that strain H23T48T was closely related to the genus Flaviflexus, with 97.0 and 96.7 % sequence similarities to Flaviflexus salsibiostraticola EBR4-1-2T and Flaviflexus huanghaiensis H5T, respectively.
32886601	9	58	theme	Flaviflexus	1433:1443	arg1	sp					1454:1455	the name Flaviflexus ciconiae sp	1424:1455	the name Flaviflexus ciconiae sp	1424:1455	Based on the obtained data, strain H23T48T represents a novel species of the genus Flaviflexus, for which the name Flaviflexus ciconiae sp.
32886601	4	59	dep	%	594:594	arg1	similarities					605:616	sequence similarities	596:616	97.0 and 96.7 % sequence similarities to Flaviflexus salsibiostraticola EBR4-1-2T	580:660	16S rRNA gene sequence-based phylogenetic analysis revealed that strain H23T48T was closely related to the genus Flaviflexus, with 97.0 and 96.7 % sequence similarities to Flaviflexus salsibiostraticola EBR4-1-2T and Flaviflexus huanghaiensis H5T, respectively.
32886601	9	60	theme	name	1428:1431	arg1	sp					1454:1455	the name Flaviflexus ciconiae sp	1424:1455	the name Flaviflexus ciconiae sp	1424:1455	Based on the obtained data, strain H23T48T represents a novel species of the genus Flaviflexus, for which the name Flaviflexus ciconiae sp.
32886601	3	61	dep	%	435:435	arg1	w/v					438:440	w/v	438:440	w/v	438:440	Optimal growth of strain H23T48T was observed at 30-37 °C, pH 8 and with 3 % (w/v) NaCl.
32886601	8	62	theme	H23T48T	1177:1183	arg1	content					1159:1165	The genomic G+C content	1143:1165	The genomic G+C content of strain H23T48T	1143:1183	The genomic G+C content of strain H23T48T is 59.5 mol% and the average nucleotide identity value between H23T48T and F. salsibiostraticola KCT C33148T (=EBR4-1-2T) is 75.5 %.
32886601	8	62	theme	H23T48T	1177:1183	arg1	%					1196:1196	59.5 mol%	1188:1196	59.5 mol%	1188:1196	The genomic G+C content of strain H23T48T is 59.5 mol% and the average nucleotide identity value between H23T48T and F. salsibiostraticola KCT C33148T (=EBR4-1-2T) is 75.5 %.
32886601	8	63	theme	strain	1170:1175	arg1	H23T48T					1177:1183	strain H23T48T	1170:1183	strain H23T48T	1170:1183	The genomic G+C content of strain H23T48T is 59.5 mol% and the average nucleotide identity value between H23T48T and F. salsibiostraticola KCT C33148T (=EBR4-1-2T) is 75.5 %.
32886601	6	64	theme	unidentified	946:957	arg1	phosphatidylglycerol					895:914	phosphatidylglycerol	895:914	phosphatidylglycerol	895:914	The polar lipids included phosphatidylglycerol, two unidentified lipids, six unidentified phospholipids and two unidentified glycophospholipids.
32886601	6	64	theme	unidentified	946:957	arg1	phospholipids					959:971	six unidentified phospholipids	942:971	six unidentified phospholipids	942:971	The polar lipids included phosphatidylglycerol, two unidentified lipids, six unidentified phospholipids and two unidentified glycophospholipids.
32886601	7	65	theme	d-glutamic	1097:1106	arg1	acid					1108:1111	d-glutamic acid	1097:1111	d-glutamic acid	1097:1111	The amino acid composition of the cell-wall peptidoglycan was l-alanine, l-lysine, d-glutamic acid, l-aspartic acid and glycine.
32886601	5	66	theme	Strain	711:716	arg1	H23T48T					718:724	Strain H23T48T	711:724	Strain H23T48T	711:724	Strain H23T48T possessed MK-9(H4) as the major menaquinone and C16 : 0 (42.4 %), C18 : 1 ω9c (31.3 %) and C14 : 0 (17.7 %) as the major cellular fatty acids.
32886601	2	67	theme	novel	99:103	arg1	H23T48T					225:231	designated strain H23T48T	207:231	designated strain H23T48T	207:231	A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium, designated strain H23T48T, was isolated from the faecal sample of an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea.
32886601	2	67	theme	novel	99:103	arg1	bacterium					196:204	A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium	97:204	A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium	97:204	A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium, designated strain H23T48T, was isolated from the faecal sample of an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea.
32886601	5	68	theme	major	841:845	arg1	acids					862:866	the major cellular fatty acids	837:866	the major cellular fatty acids	837:866	Strain H23T48T possessed MK-9(H4) as the major menaquinone and C16 : 0 (42.4 %), C18 : 1 ω9c (31.3 %) and C14 : 0 (17.7 %) as the major cellular fatty acids.
32886601	4	69	theme	sequence	596:603	arg1	similarities					605:616	sequence similarities	596:616	97.0 and 96.7 % sequence similarities to Flaviflexus salsibiostraticola EBR4-1-2T	580:660	16S rRNA gene sequence-based phylogenetic analysis revealed that strain H23T48T was closely related to the genus Flaviflexus, with 97.0 and 96.7 % sequence similarities to Flaviflexus salsibiostraticola EBR4-1-2T and Flaviflexus huanghaiensis H5T, respectively.
32886601	11	70	theme	49253T=JCM	1510:1519	arg1	33282T					1521:1526	=KCTC 49253T=JCM 33282T	1504:1526	=KCTC 49253T=JCM 33282T	1504:1526	The type strain is H23T48T (=KCTC 49253T=JCM 33282T).
32886601	11	70	theme	49253T=JCM	1510:1519	arg1	H23T48T					1495:1501	H23T48T	1495:1501	H23T48T (=KCTC 49253T=JCM 33282T)	1495:1527	The type strain is H23T48T (=KCTC 49253T=JCM 33282T).
32886601	2	71	attach	isolated	238:245	arg2	bacterium					196:204	A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium	97:204	A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium	97:204	A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium, designated strain H23T48T, was isolated from the faecal sample of an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea.
32886601	2	71	attach	isolated	238:245	arg1	sample					263:268	the faecal sample	252:268	the faecal sample of an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea	252:357	A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium, designated strain H23T48T, was isolated from the faecal sample of an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea.
32886601	2	71	attach	isolated	238:245	arg2	H23T48T					225:231	designated strain H23T48T	207:231	designated strain H23T48T	207:231	A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium, designated strain H23T48T, was isolated from the faecal sample of an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea.
34684428	10	0	from	aspirates	1730:1738	arg1	timepoint					1800:1808	each timepoint	1795:1808	each timepoint	1795:1808	By collecting human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint, we can follow digestion of human milk from the breast of the mother throughout the gastrointestinal tract of the infant, or 'From Mum to Bum'.
34684428	0	1	theme	Preterm	133:139	arg1	Infant					141:146	Preterm Infant	133:146	Preterm Infant	133:146	From Mum to Bum: An Observational Study Protocol to Follow Digestion of Human Milk Oligosaccharides and Glycoproteins from Mother to Preterm Infant.
34684428	3	2	from	glycoproteins	661:673	arg1	milk					684:687	human milk	678:687	human milk	678:687	Therefore, the main objective of the proposed research is to investigate how the intestinal microbiota of preterm and full-term infants differ in their ability to extract energy and nutrients from oligosaccharides and glycoproteins in human milk.
34684428	3	2	from	glycoproteins	661:673	arg1	nutrients					625:633	nutrients	625:633	nutrients	625:633	Therefore, the main objective of the proposed research is to investigate how the intestinal microbiota of preterm and full-term infants differ in their ability to extract energy and nutrients from oligosaccharides and glycoproteins in human milk.
34684428	3	2	from	glycoproteins	661:673	arg1	energy					614:619	extract energy	606:619	extract energy	606:619	Therefore, the main objective of the proposed research is to investigate how the intestinal microbiota of preterm and full-term infants differ in their ability to extract energy and nutrients from oligosaccharides and glycoproteins in human milk.
34684428	10	3	theme	samples	1769:1775	arg1	milk					1701:1704	human milk	1695:1704	human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint	1695:1808	By collecting human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint, we can follow digestion of human milk from the breast of the mother throughout the gastrointestinal tract of the infant, or 'From Mum to Bum'.
34684428	2	4	theme	gastrointestinal	320:335	arg1	tract					337:341	the gastrointestinal tract	316:341	the gastrointestinal tract	316:341	Aberrant maturation of the gastrointestinal tract and the microbiota could affect the digestion of human milk and its nutritional value considerably.
34684428	6	5	theme	fecal	1182:1186	arg1	samples					1188:1194	fecal samples	1182:1194	fecal samples of all infants	1182:1209	We will collect human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants.
34684428	10	6	theme	infant	1924:1929	arg1	tract					1911:1915	the gastrointestinal tract	1890:1915	the gastrointestinal tract of the infant	1890:1929	By collecting human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint, we can follow digestion of human milk from the breast of the mother throughout the gastrointestinal tract of the infant, or 'From Mum to Bum'.
34684428	6	7	theme	preterm	1162:1168	arg1	infants					1170:1176	preterm infants	1162:1176	preterm infants	1162:1176	We will collect human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants.
34684428	7	8	theme	calorimetric	1310:1321	arg1	measurements					1323:1334	calorimetric measurements	1310:1334	calorimetric measurements	1310:1334	A combination of 16S rRNA amplicon sequencing, proteomics, peptidomics, carbohydrate analysis and calorimetric measurements will be performed.
34684428	1	9	theme	nutritional	153:163	arg1	requirements					165:176	The nutritional requirements	149:176	The nutritional requirements of preterm infants	149:195	The nutritional requirements of preterm infants are challenging to meet in neonatal care, yet crucial for their growth, development and health.
34684428	10	10	from	samples	1769:1775	arg1	timepoint					1800:1808	each timepoint	1795:1808	each timepoint	1795:1808	By collecting human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint, we can follow digestion of human milk from the breast of the mother throughout the gastrointestinal tract of the infant, or 'From Mum to Bum'.
34684428	7	11	theme	analysis	1297:1304	arg1	combination					1214:1224	A combination	1212:1224	A combination of 16S rRNA amplicon sequencing, proteomics, peptidomics, carbohydrate analysis and calorimetric measurements	1212:1334	A combination of 16S rRNA amplicon sequencing, proteomics, peptidomics, carbohydrate analysis and calorimetric measurements will be performed.
34684428	3	12	theme	preterm	549:555	arg1	infants					571:577	preterm and full-term infants	549:577	preterm and full-term infants	549:577	Therefore, the main objective of the proposed research is to investigate how the intestinal microbiota of preterm and full-term infants differ in their ability to extract energy and nutrients from oligosaccharides and glycoproteins in human milk.
34684428	9	13	theme	first	1521:1525	arg1	study					1527:1531	the first study	1517:1531	the first study in which the effect of a preterm gut microbiota composition on its metabolic capacity and subsequent infant growth and development is investigated	1517:1678	The 'From Mum to Bum' study is the first study in which the effect of a preterm gut microbiota composition on its metabolic capacity and subsequent infant growth and development is investigated.
34684428	9	13	theme	first	1521:1525	arg1	The					1486:1488	The	1486:1488	The	1486:1488	The 'From Mum to Bum' study is the first study in which the effect of a preterm gut microbiota composition on its metabolic capacity and subsequent infant growth and development is investigated.
34684428	9	14	dep	study	1508:1512	arg1	to					1500:1501	to	1500:1501	to	1500:1501	The 'From Mum to Bum' study is the first study in which the effect of a preterm gut microbiota composition on its metabolic capacity and subsequent infant growth and development is investigated.
34684428	3	15	theme	proposed	480:487	arg1	research					489:496	the proposed research	476:496	the proposed research	476:496	Therefore, the main objective of the proposed research is to investigate how the intestinal microbiota of preterm and full-term infants differ in their ability to extract energy and nutrients from oligosaccharides and glycoproteins in human milk.
34684428	1	16	theme	infants	189:195	arg1	requirements					165:176	The nutritional requirements	149:176	The nutritional requirements of preterm infants	149:195	The nutritional requirements of preterm infants are challenging to meet in neonatal care, yet crucial for their growth, development and health.
34684428	9	17	theme	gut	1566:1568	arg1	composition					1581:1591	a preterm gut microbiota composition	1556:1591	a preterm gut microbiota composition	1556:1591	The 'From Mum to Bum' study is the first study in which the effect of a preterm gut microbiota composition on its metabolic capacity and subsequent infant growth and development is investigated.
34684428	6	18	theme	mothers	1132:1138	arg1	aspirates					1149:1157	gastric aspirates	1141:1157	gastric aspirates of preterm infants	1141:1176	We will collect human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants.
34684428	6	18	theme	mothers	1132:1138	arg1	samples					1188:1194	fecal samples	1182:1194	fecal samples of all infants	1182:1209	We will collect human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants.
34684428	6	18	theme	mothers	1132:1138	arg1	milk					1120:1123	human milk	1114:1123	human milk of all mothers	1114:1138	We will collect human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants.
34684428	10	19	theme	milk	1844:1847	arg1	digestion					1825:1833	digestion	1825:1833	digestion of human milk	1825:1847	By collecting human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint, we can follow digestion of human milk from the breast of the mother throughout the gastrointestinal tract of the infant, or 'From Mum to Bum'.
34684428	10	20	from	timepoint	1800:1808	arg1	mothers					1713:1719	all mothers	1709:1719	all mothers	1709:1719	By collecting human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint, we can follow digestion of human milk from the breast of the mother throughout the gastrointestinal tract of the infant, or 'From Mum to Bum'.
34684428	10	20	from	timepoint	1800:1808	arg1	aspirates					1730:1738	gastric aspirates	1722:1738	gastric aspirates of preterm infants	1722:1757	By collecting human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint, we can follow digestion of human milk from the breast of the mother throughout the gastrointestinal tract of the infant, or 'From Mum to Bum'.
34684428	10	20	from	timepoint	1800:1808	arg1	samples					1769:1775	fecal samples	1763:1775	fecal samples of all infants at each timepoint	1763:1808	By collecting human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint, we can follow digestion of human milk from the breast of the mother throughout the gastrointestinal tract of the infant, or 'From Mum to Bum'.
34684428	10	20	from	timepoint	1800:1808	arg1	infants					1784:1790	all infants	1780:1790	all infants at each timepoint	1780:1808	By collecting human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint, we can follow digestion of human milk from the breast of the mother throughout the gastrointestinal tract of the infant, or 'From Mum to Bum'.
34684428	9	21	theme	composition	1581:1591	arg1	effect					1546:1551	the effect	1542:1551	the effect of a preterm gut microbiota composition on its metabolic capacity and subsequent infant growth and development	1542:1662	The 'From Mum to Bum' study is the first study in which the effect of a preterm gut microbiota composition on its metabolic capacity and subsequent infant growth and development is investigated.
34684428	4	22	theme	pilot	695:699	arg1	study					747:751	an observational, single-center study	715:751	study	747:751	This pilot study will be an observational, single-center study performed at the Neonatal Intensive Care Unit at Isala Women and Children's Hospital (Zwolle, The Netherlands).
34684428	4	22	theme	pilot	695:699	arg1	study					701:705	This pilot study	690:705	This pilot study	690:705	This pilot study will be an observational, single-center study performed at the Neonatal Intensive Care Unit at Isala Women and Children's Hospital (Zwolle, The Netherlands).
34684428	10	23	theme	fecal	1763:1767	arg1	samples					1769:1775	fecal samples	1763:1775	fecal samples of all infants at each timepoint	1763:1808	By collecting human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint, we can follow digestion of human milk from the breast of the mother throughout the gastrointestinal tract of the infant, or 'From Mum to Bum'.
34684428	5	24	theme	gestation	970:978	arg1	weeks					961:965	full-term 37-42 weeks	945:965	full-term 37-42 weeks of gestation	945:978	A cohort of thirty mother-infant pairs (preterm ≤30 weeks of gestation, n = 15; full-term 37-42 weeks of gestation, n = 15) will be followed during the first six postnatal weeks with follow-up at three- and six-months postnatal age.
34684428	3	25	theme	full-term	561:569	arg1	infants					571:577	preterm and full-term infants	549:577	preterm and full-term infants	549:577	Therefore, the main objective of the proposed research is to investigate how the intestinal microbiota of preterm and full-term infants differ in their ability to extract energy and nutrients from oligosaccharides and glycoproteins in human milk.
34684428	6	26	theme	human	1114:1118	arg1	milk					1120:1123	human milk	1114:1123	human milk of all mothers	1114:1138	We will collect human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants.
34684428	7	27	theme	amplicon	1238:1245	arg1	sequencing					1247:1256	16S rRNA amplicon sequencing	1229:1256	16S rRNA amplicon sequencing	1229:1256	A combination of 16S rRNA amplicon sequencing, proteomics, peptidomics, carbohydrate analysis and calorimetric measurements will be performed.
34684428	5	28	dep	weeks	917:921	arg1	n					981:981	n = 15	981:986	n = 15	981:986	A cohort of thirty mother-infant pairs (preterm ≤30 weeks of gestation, n = 15; full-term 37-42 weeks of gestation, n = 15) will be followed during the first six postnatal weeks with follow-up at three- and six-months postnatal age.
34684428	5	28	dep	weeks	917:921	arg1	n					937:937	n = 15	937:942	n = 15	937:942	A cohort of thirty mother-infant pairs (preterm ≤30 weeks of gestation, n = 15; full-term 37-42 weeks of gestation, n = 15) will be followed during the first six postnatal weeks with follow-up at three- and six-months postnatal age.
34684428	5	28	dep	weeks	917:921	arg1	weeks					961:965	full-term 37-42 weeks	945:965	full-term 37-42 weeks of gestation	945:978	A cohort of thirty mother-infant pairs (preterm ≤30 weeks of gestation, n = 15; full-term 37-42 weeks of gestation, n = 15) will be followed during the first six postnatal weeks with follow-up at three- and six-months postnatal age.
34684428	5	29	theme	mother-infant	884:896	arg1	pairs					898:902	thirty mother-infant pairs	877:902	thirty mother-infant pairs (preterm ≤30 weeks of gestation, n = 15; full-term 37-42 weeks of gestation, n = 15)	877:987	A cohort of thirty mother-infant pairs (preterm ≤30 weeks of gestation, n = 15; full-term 37-42 weeks of gestation, n = 15) will be followed during the first six postnatal weeks with follow-up at three- and six-months postnatal age.
34684428	5	29	theme	mother-infant	884:896	arg1	weeks					917:921	preterm ≤30 weeks	905:921	preterm ≤30 weeks of gestation	905:934	A cohort of thirty mother-infant pairs (preterm ≤30 weeks of gestation, n = 15; full-term 37-42 weeks of gestation, n = 15) will be followed during the first six postnatal weeks with follow-up at three- and six-months postnatal age.
34684428	8	30	theme	microbiota	1371:1380	arg1	role					1359:1362	The role	1355:1362	The role of the microbiota in infant growth and development	1355:1413	The role of the microbiota in infant growth and development is often overlooked yet offers opportunities to advance neonatal care.
34684428	7	31	theme	16S	1229:1231	arg1	sequencing					1247:1256	16S rRNA amplicon sequencing	1229:1256	16S rRNA amplicon sequencing	1229:1256	A combination of 16S rRNA amplicon sequencing, proteomics, peptidomics, carbohydrate analysis and calorimetric measurements will be performed.
34684428	10	32	theme	human	1695:1699	arg1	milk					1701:1704	human milk	1695:1704	human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint	1695:1808	By collecting human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint, we can follow digestion of human milk from the breast of the mother throughout the gastrointestinal tract of the infant, or 'From Mum to Bum'.
34684428	9	33	theme	subsequent	1623:1632	arg1	growth					1641:1646	subsequent infant growth	1623:1646	subsequent infant growth	1623:1646	The 'From Mum to Bum' study is the first study in which the effect of a preterm gut microbiota composition on its metabolic capacity and subsequent infant growth and development is investigated.
34684428	8	34	theme	infant	1385:1390	arg1	growth					1392:1397	infant growth	1385:1397	infant growth	1385:1397	The role of the microbiota in infant growth and development is often overlooked yet offers opportunities to advance neonatal care.
34684428	10	35	theme	mother	1872:1877	arg1	breast					1858:1863	the breast	1854:1863	the breast of the mother	1854:1877	By collecting human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint, we can follow digestion of human milk from the breast of the mother throughout the gastrointestinal tract of the infant, or 'From Mum to Bum'.
34684428	0	36	theme	Milk	78:81	arg1	Oligosaccharides					83:98	Human Milk Oligosaccharides	72:98	Human Milk Oligosaccharides	72:98	From Mum to Bum: An Observational Study Protocol to Follow Digestion of Human Milk Oligosaccharides and Glycoproteins from Mother to Preterm Infant.
34684428	2	37	theme	value	423:427	arg1	digestion					379:387	the digestion	375:387	the digestion of human milk and its nutritional value	375:427	Aberrant maturation of the gastrointestinal tract and the microbiota could affect the digestion of human milk and its nutritional value considerably.
34684428	0	38	dep	Follow	52:57	arg1	to					49:50	to	49:50	to	49:50	From Mum to Bum: An Observational Study Protocol to Follow Digestion of Human Milk Oligosaccharides and Glycoproteins from Mother to Preterm Infant.
34684428	2	39	theme	milk	398:401	arg1	digestion					379:387	the digestion	375:387	the digestion of human milk and its nutritional value	375:427	Aberrant maturation of the gastrointestinal tract and the microbiota could affect the digestion of human milk and its nutritional value considerably.
34684428	10	40	theme	gastric	1722:1728	arg1	aspirates					1730:1738	gastric aspirates	1722:1738	gastric aspirates of preterm infants	1722:1757	By collecting human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint, we can follow digestion of human milk from the breast of the mother throughout the gastrointestinal tract of the infant, or 'From Mum to Bum'.
34684428	5	41	theme	follow-up	1048:1056	arg1	age					1093:1095	follow-up at three- and six-months postnatal age	1048:1095	follow-up at three- and six-months postnatal age	1048:1095	A cohort of thirty mother-infant pairs (preterm ≤30 weeks of gestation, n = 15; full-term 37-42 weeks of gestation, n = 15) will be followed during the first six postnatal weeks with follow-up at three- and six-months postnatal age.
34684428	8	42	theme	neonatal	1471:1478	arg1	care					1480:1483	neonatal care	1471:1483	neonatal care	1471:1483	The role of the microbiota in infant growth and development is often overlooked yet offers opportunities to advance neonatal care.
34684428	10	43	theme	mothers	1713:1719	arg1	milk					1701:1704	human milk	1695:1704	human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint	1695:1808	By collecting human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint, we can follow digestion of human milk from the breast of the mother throughout the gastrointestinal tract of the infant, or 'From Mum to Bum'.
34684428	0	44	theme	Observational	20:32	arg1	Protocol					40:47	An Observational Study Protocol	17:47	Bum: An Observational Study Protocol	12:47	From Mum to Bum: An Observational Study Protocol to Follow Digestion of Human Milk Oligosaccharides and Glycoproteins from Mother to Preterm Infant.
34684428	10	45	theme	infants	1751:1757	arg1	mothers					1713:1719	all mothers	1709:1719	all mothers	1709:1719	By collecting human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint, we can follow digestion of human milk from the breast of the mother throughout the gastrointestinal tract of the infant, or 'From Mum to Bum'.
34684428	10	45	theme	infants	1751:1757	arg1	aspirates					1730:1738	gastric aspirates	1722:1738	gastric aspirates of preterm infants	1722:1757	By collecting human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint, we can follow digestion of human milk from the breast of the mother throughout the gastrointestinal tract of the infant, or 'From Mum to Bum'.
34684428	10	45	theme	infants	1751:1757	arg1	samples					1769:1775	fecal samples	1763:1775	fecal samples of all infants at each timepoint	1763:1808	By collecting human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint, we can follow digestion of human milk from the breast of the mother throughout the gastrointestinal tract of the infant, or 'From Mum to Bum'.
34684428	4	46	theme	Neonatal	770:777	arg1	Unit					794:797	the Neonatal Intensive Care Unit	766:797	the Neonatal Intensive Care Unit	766:797	This pilot study will be an observational, single-center study performed at the Neonatal Intensive Care Unit at Isala Women and Children's Hospital (Zwolle, The Netherlands).
34684428	5	47	theme	=	939:939	arg1	n					937:937	n = 15	937:942	n = 15	937:942	A cohort of thirty mother-infant pairs (preterm ≤30 weeks of gestation, n = 15; full-term 37-42 weeks of gestation, n = 15) will be followed during the first six postnatal weeks with follow-up at three- and six-months postnatal age.
34684428	4	48	theme	Care	789:792	arg1	Unit					794:797	the Neonatal Intensive Care Unit	766:797	the Neonatal Intensive Care Unit	766:797	This pilot study will be an observational, single-center study performed at the Neonatal Intensive Care Unit at Isala Women and Children's Hospital (Zwolle, The Netherlands).
34684428	3	49	theme	research	489:496	arg1	objective					463:471	the main objective	454:471	the main objective of the proposed research	454:496	Therefore, the main objective of the proposed research is to investigate how the intestinal microbiota of preterm and full-term infants differ in their ability to extract energy and nutrients from oligosaccharides and glycoproteins in human milk.
34684428	10	50	from	mothers	1713:1719	arg1	timepoint					1800:1808	each timepoint	1795:1808	each timepoint	1795:1808	By collecting human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint, we can follow digestion of human milk from the breast of the mother throughout the gastrointestinal tract of the infant, or 'From Mum to Bum'.
34684428	10	51	dep	Bum	1948:1950	arg1	Mum					1941:1943	Mum	1941:1943	Mum	1941:1943	By collecting human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint, we can follow digestion of human milk from the breast of the mother throughout the gastrointestinal tract of the infant, or 'From Mum to Bum'.
34684428	5	52	from	three-	1061:1066	arg1	follow-up					1048:1056	follow-up	1048:1056	follow-up	1048:1056	A cohort of thirty mother-infant pairs (preterm ≤30 weeks of gestation, n = 15; full-term 37-42 weeks of gestation, n = 15) will be followed during the first six postnatal weeks with follow-up at three- and six-months postnatal age.
34684428	2	53	theme	tract	337:341	arg1	maturation					302:311	Aberrant maturation	293:311	Aberrant maturation of the gastrointestinal tract and the microbiota	293:360	Aberrant maturation of the gastrointestinal tract and the microbiota could affect the digestion of human milk and its nutritional value considerably.
34684428	5	54	from	six-months	1072:1081	arg1	follow-up					1048:1056	follow-up	1048:1056	follow-up	1048:1056	A cohort of thirty mother-infant pairs (preterm ≤30 weeks of gestation, n = 15; full-term 37-42 weeks of gestation, n = 15) will be followed during the first six postnatal weeks with follow-up at three- and six-months postnatal age.
34684428	3	55	theme	human	678:682	arg1	milk					684:687	human milk	678:687	human milk	678:687	Therefore, the main objective of the proposed research is to investigate how the intestinal microbiota of preterm and full-term infants differ in their ability to extract energy and nutrients from oligosaccharides and glycoproteins in human milk.
34684428	6	56	theme	infants	1170:1176	arg1	aspirates					1149:1157	gastric aspirates	1141:1157	gastric aspirates of preterm infants	1141:1176	We will collect human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants.
34684428	6	56	theme	infants	1170:1176	arg1	samples					1188:1194	fecal samples	1182:1194	fecal samples of all infants	1182:1209	We will collect human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants.
34684428	6	56	theme	infants	1170:1176	arg1	milk					1120:1123	human milk	1114:1123	human milk of all mothers	1114:1138	We will collect human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants.
34684428	7	57	theme	measurements	1323:1334	arg1	combination					1214:1224	A combination	1212:1224	A combination of 16S rRNA amplicon sequencing, proteomics, peptidomics, carbohydrate analysis and calorimetric measurements	1212:1334	A combination of 16S rRNA amplicon sequencing, proteomics, peptidomics, carbohydrate analysis and calorimetric measurements will be performed.
34684428	10	58	theme	infants	1784:1790	arg1	mothers					1713:1719	all mothers	1709:1719	all mothers	1709:1719	By collecting human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint, we can follow digestion of human milk from the breast of the mother throughout the gastrointestinal tract of the infant, or 'From Mum to Bum'.
34684428	10	58	theme	infants	1784:1790	arg1	aspirates					1730:1738	gastric aspirates	1722:1738	gastric aspirates of preterm infants	1722:1757	By collecting human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint, we can follow digestion of human milk from the breast of the mother throughout the gastrointestinal tract of the infant, or 'From Mum to Bum'.
34684428	10	58	theme	infants	1784:1790	arg1	samples					1769:1775	fecal samples	1763:1775	fecal samples of all infants at each timepoint	1763:1808	By collecting human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint, we can follow digestion of human milk from the breast of the mother throughout the gastrointestinal tract of the infant, or 'From Mum to Bum'.
34684428	6	59	theme	gastric	1141:1147	arg1	aspirates					1149:1157	gastric aspirates	1141:1157	gastric aspirates of preterm infants	1141:1176	We will collect human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants.
34684428	9	60	from	effect	1546:1551	arg1	capacity					1610:1617	its metabolic capacity	1596:1617	its metabolic capacity	1596:1617	The 'From Mum to Bum' study is the first study in which the effect of a preterm gut microbiota composition on its metabolic capacity and subsequent infant growth and development is investigated.
34684428	9	60	from	effect	1546:1551	arg1	growth					1641:1646	subsequent infant growth	1623:1646	subsequent infant growth	1623:1646	The 'From Mum to Bum' study is the first study in which the effect of a preterm gut microbiota composition on its metabolic capacity and subsequent infant growth and development is investigated.
34684428	0	61	theme	Glycoproteins	104:116	arg1	Digestion					59:67	Digestion	59:67	Digestion of Human Milk Oligosaccharides and Glycoproteins	59:116	From Mum to Bum: An Observational Study Protocol to Follow Digestion of Human Milk Oligosaccharides and Glycoproteins from Mother to Preterm Infant.
34684428	3	62	theme	intestinal	524:533	arg1	microbiota					535:544	the intestinal microbiota	520:544	the intestinal microbiota of preterm and full-term infants	520:577	Therefore, the main objective of the proposed research is to investigate how the intestinal microbiota of preterm and full-term infants differ in their ability to extract energy and nutrients from oligosaccharides and glycoproteins in human milk.
34684428	1	63	theme	preterm	181:187	arg1	infants					189:195	preterm infants	181:195	preterm infants	181:195	The nutritional requirements of preterm infants are challenging to meet in neonatal care, yet crucial for their growth, development and health.
34684428	9	64	theme	preterm	1558:1564	arg1	composition					1581:1591	a preterm gut microbiota composition	1556:1591	a preterm gut microbiota composition	1556:1591	The 'From Mum to Bum' study is the first study in which the effect of a preterm gut microbiota composition on its metabolic capacity and subsequent infant growth and development is investigated.
34684428	7	65	theme	carbohydrate	1284:1295	arg1	analysis					1297:1304	carbohydrate analysis	1284:1304	carbohydrate analysis	1284:1304	A combination of 16S rRNA amplicon sequencing, proteomics, peptidomics, carbohydrate analysis and calorimetric measurements will be performed.
34684428	3	66	theme	infants	571:577	arg1	microbiota					535:544	the intestinal microbiota	520:544	the intestinal microbiota of preterm and full-term infants	520:577	Therefore, the main objective of the proposed research is to investigate how the intestinal microbiota of preterm and full-term infants differ in their ability to extract energy and nutrients from oligosaccharides and glycoproteins in human milk.
34684428	9	67	theme	microbiota	1570:1579	arg1	composition					1581:1591	a preterm gut microbiota composition	1556:1591	a preterm gut microbiota composition	1556:1591	The 'From Mum to Bum' study is the first study in which the effect of a preterm gut microbiota composition on its metabolic capacity and subsequent infant growth and development is investigated.
34684428	7	68	theme	peptidomics	1271:1281	arg1	combination					1214:1224	A combination	1212:1224	A combination of 16S rRNA amplicon sequencing, proteomics, peptidomics, carbohydrate analysis and calorimetric measurements	1212:1334	A combination of 16S rRNA amplicon sequencing, proteomics, peptidomics, carbohydrate analysis and calorimetric measurements will be performed.
34684428	10	69	theme	human	1838:1842	arg1	milk					1844:1847	human milk	1838:1847	human milk	1838:1847	By collecting human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint, we can follow digestion of human milk from the breast of the mother throughout the gastrointestinal tract of the infant, or 'From Mum to Bum'.
34684428	6	70	theme	infants	1203:1209	arg1	aspirates					1149:1157	gastric aspirates	1141:1157	gastric aspirates of preterm infants	1141:1176	We will collect human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants.
34684428	6	70	theme	infants	1203:1209	arg1	samples					1188:1194	fecal samples	1182:1194	fecal samples of all infants	1182:1209	We will collect human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants.
34684428	6	70	theme	infants	1203:1209	arg1	milk					1120:1123	human milk	1114:1123	human milk of all mothers	1114:1138	We will collect human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants.
34684428	4	71	from	Isala	802:806	arg1	study					747:751	an observational, single-center study	715:751	study	747:751	This pilot study will be an observational, single-center study performed at the Neonatal Intensive Care Unit at Isala Women and Children's Hospital (Zwolle, The Netherlands).
34684428	4	71	from	Isala	802:806	arg1	study					701:705	This pilot study	690:705	This pilot study	690:705	This pilot study will be an observational, single-center study performed at the Neonatal Intensive Care Unit at Isala Women and Children's Hospital (Zwolle, The Netherlands).
34684428	7	72	theme	proteomics	1259:1268	arg1	combination					1214:1224	A combination	1212:1224	A combination of 16S rRNA amplicon sequencing, proteomics, peptidomics, carbohydrate analysis and calorimetric measurements	1212:1334	A combination of 16S rRNA amplicon sequencing, proteomics, peptidomics, carbohydrate analysis and calorimetric measurements will be performed.
34684428	9	73	theme	metabolic	1600:1608	arg1	capacity					1610:1617	its metabolic capacity	1596:1617	its metabolic capacity	1596:1617	The 'From Mum to Bum' study is the first study in which the effect of a preterm gut microbiota composition on its metabolic capacity and subsequent infant growth and development is investigated.
34684428	7	74	theme	sequencing	1247:1256	arg1	combination					1214:1224	A combination	1212:1224	A combination of 16S rRNA amplicon sequencing, proteomics, peptidomics, carbohydrate analysis and calorimetric measurements	1212:1334	A combination of 16S rRNA amplicon sequencing, proteomics, peptidomics, carbohydrate analysis and calorimetric measurements will be performed.
34684428	8	75	from	role	1359:1362	arg1	development					1403:1413	development	1403:1413	development	1403:1413	The role of the microbiota in infant growth and development is often overlooked yet offers opportunities to advance neonatal care.
34684428	8	75	from	role	1359:1362	arg1	growth					1392:1397	infant growth	1385:1397	infant growth	1385:1397	The role of the microbiota in infant growth and development is often overlooked yet offers opportunities to advance neonatal care.
34684428	5	76	theme	=	983:983	arg1	n					981:981	n = 15	981:986	n = 15	981:986	A cohort of thirty mother-infant pairs (preterm ≤30 weeks of gestation, n = 15; full-term 37-42 weeks of gestation, n = 15) will be followed during the first six postnatal weeks with follow-up at three- and six-months postnatal age.
34684428	7	77	theme	rRNA	1233:1236	arg1	sequencing					1247:1256	16S rRNA amplicon sequencing	1229:1256	16S rRNA amplicon sequencing	1229:1256	A combination of 16S rRNA amplicon sequencing, proteomics, peptidomics, carbohydrate analysis and calorimetric measurements will be performed.
34684428	5	78	with	weeks	1037:1041	arg1	age					1093:1095	follow-up at three- and six-months postnatal age	1048:1095	follow-up at three- and six-months postnatal age	1048:1095	A cohort of thirty mother-infant pairs (preterm ≤30 weeks of gestation, n = 15; full-term 37-42 weeks of gestation, n = 15) will be followed during the first six postnatal weeks with follow-up at three- and six-months postnatal age.
34684428	5	79	theme	postnatal	1027:1035	arg1	weeks					1037:1041	the first six postnatal weeks	1013:1041	the first six postnatal weeks with follow-up at three- and six-months postnatal age	1013:1095	A cohort of thirty mother-infant pairs (preterm ≤30 weeks of gestation, n = 15; full-term 37-42 weeks of gestation, n = 15) will be followed during the first six postnatal weeks with follow-up at three- and six-months postnatal age.
34684428	9	80	theme	infant	1634:1639	arg1	growth					1641:1646	subsequent infant growth	1623:1646	subsequent infant growth	1623:1646	The 'From Mum to Bum' study is the first study in which the effect of a preterm gut microbiota composition on its metabolic capacity and subsequent infant growth and development is investigated.
34684428	0	81	theme	Human	72:76	arg1	Oligosaccharides					83:98	Human Milk Oligosaccharides	72:98	Human Milk Oligosaccharides	72:98	From Mum to Bum: An Observational Study Protocol to Follow Digestion of Human Milk Oligosaccharides and Glycoproteins from Mother to Preterm Infant.
34684428	4	82	theme	single-center	733:745	arg1	study					747:751	an observational, single-center study	715:751	study	747:751	This pilot study will be an observational, single-center study performed at the Neonatal Intensive Care Unit at Isala Women and Children's Hospital (Zwolle, The Netherlands).
34684428	4	82	theme	single-center	733:745	arg1	study					701:705	This pilot study	690:705	This pilot study	690:705	This pilot study will be an observational, single-center study performed at the Neonatal Intensive Care Unit at Isala Women and Children's Hospital (Zwolle, The Netherlands).
34684428	2	83	theme	Aberrant	293:300	arg1	maturation					302:311	Aberrant maturation	293:311	Aberrant maturation of the gastrointestinal tract and the microbiota	293:360	Aberrant maturation of the gastrointestinal tract and the microbiota could affect the digestion of human milk and its nutritional value considerably.
34684428	0	84	theme	Oligosaccharides	83:98	arg1	Digestion					59:67	Digestion	59:67	Digestion of Human Milk Oligosaccharides and Glycoproteins	59:116	From Mum to Bum: An Observational Study Protocol to Follow Digestion of Human Milk Oligosaccharides and Glycoproteins from Mother to Preterm Infant.
34684428	4	85	dep	Isala	802:806	arg1	Women					808:812	Women	808:812	Women	808:812	This pilot study will be an observational, single-center study performed at the Neonatal Intensive Care Unit at Isala Women and Children's Hospital (Zwolle, The Netherlands).
34684428	5	86	theme	gestation	926:934	arg1	pairs					898:902	thirty mother-infant pairs	877:902	thirty mother-infant pairs (preterm ≤30 weeks of gestation, n = 15; full-term 37-42 weeks of gestation, n = 15)	877:987	A cohort of thirty mother-infant pairs (preterm ≤30 weeks of gestation, n = 15; full-term 37-42 weeks of gestation, n = 15) will be followed during the first six postnatal weeks with follow-up at three- and six-months postnatal age.
34684428	5	86	theme	gestation	926:934	arg1	weeks					917:921	preterm ≤30 weeks	905:921	preterm ≤30 weeks of gestation	905:934	A cohort of thirty mother-infant pairs (preterm ≤30 weeks of gestation, n = 15; full-term 37-42 weeks of gestation, n = 15) will be followed during the first six postnatal weeks with follow-up at three- and six-months postnatal age.
34684428	5	87	theme	first	1017:1021	arg1	weeks					1037:1041	the first six postnatal weeks	1013:1041	the first six postnatal weeks with follow-up at three- and six-months postnatal age	1013:1095	A cohort of thirty mother-infant pairs (preterm ≤30 weeks of gestation, n = 15; full-term 37-42 weeks of gestation, n = 15) will be followed during the first six postnatal weeks with follow-up at three- and six-months postnatal age.
34684428	5	88	theme	pairs	898:902	arg1	cohort					867:872	A cohort	865:872	A cohort of thirty mother-infant pairs (preterm ≤30 weeks of gestation, n = 15; full-term 37-42 weeks of gestation, n = 15)	865:987	A cohort of thirty mother-infant pairs (preterm ≤30 weeks of gestation, n = 15; full-term 37-42 weeks of gestation, n = 15) will be followed during the first six postnatal weeks with follow-up at three- and six-months postnatal age.
34684428	2	89	theme	nutritional	411:421	arg1	value					423:427	its nutritional value	407:427	its nutritional value	407:427	Aberrant maturation of the gastrointestinal tract and the microbiota could affect the digestion of human milk and its nutritional value considerably.
34684428	2	90	theme	microbiota	351:360	arg1	maturation					302:311	Aberrant maturation	293:311	Aberrant maturation of the gastrointestinal tract and the microbiota	293:360	Aberrant maturation of the gastrointestinal tract and the microbiota could affect the digestion of human milk and its nutritional value considerably.
34684428	5	91	theme	postnatal	1083:1091	arg1	age					1093:1095	follow-up at three- and six-months postnatal age	1048:1095	follow-up at three- and six-months postnatal age	1048:1095	A cohort of thirty mother-infant pairs (preterm ≤30 weeks of gestation, n = 15; full-term 37-42 weeks of gestation, n = 15) will be followed during the first six postnatal weeks with follow-up at three- and six-months postnatal age.
34684428	5	92	theme	preterm	905:911	arg1	pairs					898:902	thirty mother-infant pairs	877:902	thirty mother-infant pairs (preterm ≤30 weeks of gestation, n = 15; full-term 37-42 weeks of gestation, n = 15)	877:987	A cohort of thirty mother-infant pairs (preterm ≤30 weeks of gestation, n = 15; full-term 37-42 weeks of gestation, n = 15) will be followed during the first six postnatal weeks with follow-up at three- and six-months postnatal age.
34684428	5	92	theme	preterm	905:911	arg1	weeks					917:921	preterm ≤30 weeks	905:921	preterm ≤30 weeks of gestation	905:934	A cohort of thirty mother-infant pairs (preterm ≤30 weeks of gestation, n = 15; full-term 37-42 weeks of gestation, n = 15) will be followed during the first six postnatal weeks with follow-up at three- and six-months postnatal age.
34684428	10	93	theme	aspirates	1730:1738	arg1	milk					1701:1704	human milk	1695:1704	human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint	1695:1808	By collecting human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint, we can follow digestion of human milk from the breast of the mother throughout the gastrointestinal tract of the infant, or 'From Mum to Bum'.
34684428	4	94	theme	observational	718:730	arg1	study					747:751	an observational, single-center study	715:751	study	747:751	This pilot study will be an observational, single-center study performed at the Neonatal Intensive Care Unit at Isala Women and Children's Hospital (Zwolle, The Netherlands).
34684428	4	94	theme	observational	718:730	arg1	study					701:705	This pilot study	690:705	This pilot study	690:705	This pilot study will be an observational, single-center study performed at the Neonatal Intensive Care Unit at Isala Women and Children's Hospital (Zwolle, The Netherlands).
34684428	2	95	theme	human	392:396	arg1	milk					398:401	human milk	392:401	human milk	392:401	Aberrant maturation of the gastrointestinal tract and the microbiota could affect the digestion of human milk and its nutritional value considerably.
34684428	5	96	theme	full-term	945:953	arg1	weeks					961:965	full-term 37-42 weeks	945:965	full-term 37-42 weeks of gestation	945:978	A cohort of thirty mother-infant pairs (preterm ≤30 weeks of gestation, n = 15; full-term 37-42 weeks of gestation, n = 15) will be followed during the first six postnatal weeks with follow-up at three- and six-months postnatal age.
34684428	3	97	theme	extract	606:612	arg1	energy					614:619	extract energy	606:619	extract energy	606:619	Therefore, the main objective of the proposed research is to investigate how the intestinal microbiota of preterm and full-term infants differ in their ability to extract energy and nutrients from oligosaccharides and glycoproteins in human milk.
34684428	0	98	theme	Study	34:38	arg1	Protocol					40:47	An Observational Study Protocol	17:47	Bum: An Observational Study Protocol	12:47	From Mum to Bum: An Observational Study Protocol to Follow Digestion of Human Milk Oligosaccharides and Glycoproteins from Mother to Preterm Infant.
34684428	9	99	theme	Mum	1496:1498	arg1	study					1508:1512	Mum to Bum' study	1496:1512	Mum to Bum' study	1496:1512	The 'From Mum to Bum' study is the first study in which the effect of a preterm gut microbiota composition on its metabolic capacity and subsequent infant growth and development is investigated.
34684428	0	100	dep	Bum	12:14	arg1	Protocol					40:47	An Observational Study Protocol	17:47	Bum: An Observational Study Protocol	12:47	From Mum to Bum: An Observational Study Protocol to Follow Digestion of Human Milk Oligosaccharides and Glycoproteins from Mother to Preterm Infant.
34684428	0	100	dep	Bum	12:14	arg1	Mum					5:7	Mum	5:7	Mum	5:7	From Mum to Bum: An Observational Study Protocol to Follow Digestion of Human Milk Oligosaccharides and Glycoproteins from Mother to Preterm Infant.
34684428	3	101	gly	glycoproteins	661:673	arg1	glycoproteins					661:673	glycoproteins	661:673	glycoproteins	661:673	Therefore, the main objective of the proposed research is to investigate how the intestinal microbiota of preterm and full-term infants differ in their ability to extract energy and nutrients from oligosaccharides and glycoproteins in human milk.
34684428	10	102	theme	preterm	1743:1749	arg1	infants					1751:1757	preterm infants	1743:1757	preterm infants	1743:1757	By collecting human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint, we can follow digestion of human milk from the breast of the mother throughout the gastrointestinal tract of the infant, or 'From Mum to Bum'.
34684428	3	103	theme	main	458:461	arg1	objective					463:471	the main objective	454:471	the main objective of the proposed research	454:496	Therefore, the main objective of the proposed research is to investigate how the intestinal microbiota of preterm and full-term infants differ in their ability to extract energy and nutrients from oligosaccharides and glycoproteins in human milk.
34684428	10	104	theme	gastrointestinal	1894:1909	arg1	tract					1911:1915	the gastrointestinal tract	1890:1915	the gastrointestinal tract of the infant	1890:1929	By collecting human milk of all mothers, gastric aspirates of preterm infants and fecal samples of all infants at each timepoint, we can follow digestion of human milk from the breast of the mother throughout the gastrointestinal tract of the infant, or 'From Mum to Bum'.
34684428	3	105	from	oligosaccharides	640:655	arg1	milk					684:687	human milk	678:687	human milk	678:687	Therefore, the main objective of the proposed research is to investigate how the intestinal microbiota of preterm and full-term infants differ in their ability to extract energy and nutrients from oligosaccharides and glycoproteins in human milk.
34684428	3	105	from	oligosaccharides	640:655	arg1	nutrients					625:633	nutrients	625:633	nutrients	625:633	Therefore, the main objective of the proposed research is to investigate how the intestinal microbiota of preterm and full-term infants differ in their ability to extract energy and nutrients from oligosaccharides and glycoproteins in human milk.
34684428	3	105	from	oligosaccharides	640:655	arg1	energy					614:619	extract energy	606:619	extract energy	606:619	Therefore, the main objective of the proposed research is to investigate how the intestinal microbiota of preterm and full-term infants differ in their ability to extract energy and nutrients from oligosaccharides and glycoproteins in human milk.
34684428	4	106	theme	Intensive	779:787	arg1	Unit					794:797	the Neonatal Intensive Care Unit	766:797	the Neonatal Intensive Care Unit	766:797	This pilot study will be an observational, single-center study performed at the Neonatal Intensive Care Unit at Isala Women and Children's Hospital (Zwolle, The Netherlands).
34684428	1	107	theme	neonatal	224:231	arg1	care					233:236	neonatal care	224:236	neonatal care	224:236	The nutritional requirements of preterm infants are challenging to meet in neonatal care, yet crucial for their growth, development and health.
34339751	6	0	theme	stress	1215:1220	arg1	testing					1148:1154	behavioral testing	1137:1154	behavioral testing	1137:1154	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg improved doxorubicin-induced memory impairment in rats evidenced by behavioral testing, inhibition of acetylcholinesterase activity and oxidative stress markers; catalase, reduced glutathione, lipid peroxidation and hydrogen peroxide.
34339751	6	0	theme	stress	1215:1220	arg1	glutathione					1249:1259	reduced glutathione	1241:1259	reduced glutathione	1241:1259	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg improved doxorubicin-induced memory impairment in rats evidenced by behavioral testing, inhibition of acetylcholinesterase activity and oxidative stress markers; catalase, reduced glutathione, lipid peroxidation and hydrogen peroxide.
34339751	6	0	theme	stress	1215:1220	arg1	peroxide					1294:1301	hydrogen peroxide	1285:1301	hydrogen peroxide	1285:1301	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg improved doxorubicin-induced memory impairment in rats evidenced by behavioral testing, inhibition of acetylcholinesterase activity and oxidative stress markers; catalase, reduced glutathione, lipid peroxidation and hydrogen peroxide.
34339751	6	0	theme	stress	1215:1220	arg1	inhibition					1157:1166	inhibition	1157:1166	inhibition of acetylcholinesterase activity and oxidative stress markers	1157:1228	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg improved doxorubicin-induced memory impairment in rats evidenced by behavioral testing, inhibition of acetylcholinesterase activity and oxidative stress markers; catalase, reduced glutathione, lipid peroxidation and hydrogen peroxide.
34339751	6	0	theme	stress	1215:1220	arg1	peroxidation					1268:1279	lipid peroxidation	1262:1279	lipid peroxidation	1262:1279	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg improved doxorubicin-induced memory impairment in rats evidenced by behavioral testing, inhibition of acetylcholinesterase activity and oxidative stress markers; catalase, reduced glutathione, lipid peroxidation and hydrogen peroxide.
34339751	6	0	theme	stress	1215:1220	arg1	catalase					1231:1238	catalase	1231:1238	catalase	1231:1238	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg improved doxorubicin-induced memory impairment in rats evidenced by behavioral testing, inhibition of acetylcholinesterase activity and oxidative stress markers; catalase, reduced glutathione, lipid peroxidation and hydrogen peroxide.
34339751	6	1	theme	Intranasal	978:987	arg1	administration					989:1002	Intranasal administration	978:1002	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg	978:1067	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg improved doxorubicin-induced memory impairment in rats evidenced by behavioral testing, inhibition of acetylcholinesterase activity and oxidative stress markers; catalase, reduced glutathione, lipid peroxidation and hydrogen peroxide.
34339751	5	2	theme	present	746:752	arg1	study					754:758	the present study	742:758	the present study	742:758	In the present study, chrysin was successfully formulated as transfersomal lipid vesicles and chitosan composite vesicles (CCV) exhibiting a nanometric size range, high drug entrapment efficiency, and controlled release over a 72h period.
34339751	9	3	theme	enhanced	1812:1819	arg1	potential					1833:1841	the enhanced therapeutic potential	1808:1841	the enhanced therapeutic potential of chrysin	1808:1852	Collectively, our findings suggest the enhanced therapeutic potential of chrysin when formulated as transfersomes and CCV against chemotherapy-induced chemobrain via hindering acetylcholinesterase, oxidative stress and TLR4-NF-kB(p65)-NLRP3 pathways.
34339751	8	4	theme	chrysin	1375:1381	arg1	formulations					1383:1394	chrysin formulations	1375:1394	chrysin formulations	1375:1394	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	2	5	theme	-topoisomerase	308:321	arg1	inhibitor-					326:335	Doxorubicin -topoisomerase II inhibitor-	296:335	Doxorubicin -topoisomerase II inhibitor-	296:335	Doxorubicin -topoisomerase II inhibitor- is widely used in breast cancer, hematological cancers and other neoplasms.
34339751	1	6	theme	Many	197:200	arg1	survivors					209:217	Many cancer survivors	197:217	Many cancer survivors	197:217	Many cancer survivors suffer from chemotherapy-induced cognitive impairment known as 'Chemobrain'.
34339751	0	7	from	Insights	124:131	arg1	formulation					136:146	formulation	136:146	formulation	136:146	Nose-to-brain delivery of chrysin transfersomal and composite vesicles in doxorubicin-induced cognitive impairment in rats: Insights on formulation, oxidative stress and TLR4/NF-kB/NLRP3 pathways.
34339751	0	7	from	Insights	124:131	arg1	stress					159:164	oxidative stress	149:164	oxidative stress	149:164	Nose-to-brain delivery of chrysin transfersomal and composite vesicles in doxorubicin-induced cognitive impairment in rats: Insights on formulation, oxidative stress and TLR4/NF-kB/NLRP3 pathways.
34339751	0	7	from	Insights	124:131	arg1	pathways					187:194	TLR4/NF-kB/NLRP3 pathways	170:194	TLR4/NF-kB/NLRP3 pathways	170:194	Nose-to-brain delivery of chrysin transfersomal and composite vesicles in doxorubicin-induced cognitive impairment in rats: Insights on formulation, oxidative stress and TLR4/NF-kB/NLRP3 pathways.
34339751	9	8	theme	chrysin	1846:1852	arg1	potential					1833:1841	the enhanced therapeutic potential	1808:1841	the enhanced therapeutic potential of chrysin	1808:1852	Collectively, our findings suggest the enhanced therapeutic potential of chrysin when formulated as transfersomes and CCV against chemotherapy-induced chemobrain via hindering acetylcholinesterase, oxidative stress and TLR4-NF-kB(p65)-NLRP3 pathways.
34339751	6	9	theme	lipid	1262:1266	arg1	peroxidation					1268:1279	lipid peroxidation	1262:1279	lipid peroxidation	1262:1279	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg improved doxorubicin-induced memory impairment in rats evidenced by behavioral testing, inhibition of acetylcholinesterase activity and oxidative stress markers; catalase, reduced glutathione, lipid peroxidation and hydrogen peroxide.
34339751	5	10	theme	entrapment	913:922	arg1	efficiency					924:933	high drug entrapment efficiency	903:933	high drug entrapment efficiency	903:933	In the present study, chrysin was successfully formulated as transfersomal lipid vesicles and chitosan composite vesicles (CCV) exhibiting a nanometric size range, high drug entrapment efficiency, and controlled release over a 72h period.
34339751	6	11	theme	reduced	1043:1049	arg1	dose					1051:1054	a reduced dose	1041:1054	a reduced dose of 0.5 mg/kg	1041:1067	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg improved doxorubicin-induced memory impairment in rats evidenced by behavioral testing, inhibition of acetylcholinesterase activity and oxidative stress markers; catalase, reduced glutathione, lipid peroxidation and hydrogen peroxide.
34339751	8	12	theme	doxorubicin-induced	1417:1435	arg1	NF-kB					1547:1551	NF-kB	1547:1551	NF-kB	1547:1551	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	8	12	theme	doxorubicin-induced	1417:1435	arg1	receptor					1446:1453	doxorubicin-induced Tol-like receptor 4	1417:1455	doxorubicin-induced Tol-like receptor 4 (TLR4)	1417:1462	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	8	12	theme	doxorubicin-induced	1417:1435	arg1	TLR4					1458:1461	TLR4	1458:1461	TLR4	1458:1461	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	5	13	theme	transfersomal	800:812	arg1	chrysin					761:767	chrysin	761:767	chrysin	761:767	In the present study, chrysin was successfully formulated as transfersomal lipid vesicles and chitosan composite vesicles (CCV) exhibiting a nanometric size range, high drug entrapment efficiency, and controlled release over a 72h period.
34339751	5	13	theme	transfersomal	800:812	arg1	vesicles					820:827	transfersomal lipid vesicles	800:827	transfersomal lipid vesicles	800:827	In the present study, chrysin was successfully formulated as transfersomal lipid vesicles and chitosan composite vesicles (CCV) exhibiting a nanometric size range, high drug entrapment efficiency, and controlled release over a 72h period.
34339751	5	13	theme	transfersomal	800:812	arg1	CCV					862:864	CCV	862:864	CCV	862:864	In the present study, chrysin was successfully formulated as transfersomal lipid vesicles and chitosan composite vesicles (CCV) exhibiting a nanometric size range, high drug entrapment efficiency, and controlled release over a 72h period.
34339751	1	14	theme	cognitive	252:260	arg1	impairment					262:271	chemotherapy-induced cognitive impairment	231:271	chemotherapy-induced cognitive impairment known as 'Chemobrain'	231:293	Many cancer survivors suffer from chemotherapy-induced cognitive impairment known as 'Chemobrain'.
34339751	8	15	contain	containing	1738:1747	arg1	pyrin					1732:1736	Nod-like receptor pyrin	1714:1736	Nod-like receptor pyrin containing 3 (NLRP3) inflammasome	1714:1770	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	8	15	contain	containing	1738:1747	arg2	inflammasome					1759:1770	3 (NLRP3) inflammasome	1749:1770	3 (NLRP3) inflammasome	1749:1770	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	8	16	theme	receptor	1446:1453	arg1	expression					1562:1571	doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression	1417:1571	doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome	1417:1770	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	6	17	theme	chrysin	1017:1023	arg1	formulations					1025:1036	optimized chrysin formulations	1007:1036	optimized chrysin formulations	1007:1036	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg improved doxorubicin-induced memory impairment in rats evidenced by behavioral testing, inhibition of acetylcholinesterase activity and oxidative stress markers; catalase, reduced glutathione, lipid peroxidation and hydrogen peroxide.
34339751	0	18	theme	TLR4/NF-kB/NLRP3	170:185	arg1	pathways					187:194	TLR4/NF-kB/NLRP3 pathways	170:194	TLR4/NF-kB/NLRP3 pathways	170:194	Nose-to-brain delivery of chrysin transfersomal and composite vesicles in doxorubicin-induced cognitive impairment in rats: Insights on formulation, oxidative stress and TLR4/NF-kB/NLRP3 pathways.
34339751	9	19	theme	chemotherapy-induced	1903:1922	arg1	chemobrain					1924:1933	chemotherapy-induced chemobrain	1903:1933	chemotherapy-induced chemobrain	1903:1933	Collectively, our findings suggest the enhanced therapeutic potential of chrysin when formulated as transfersomes and CCV against chemotherapy-induced chemobrain via hindering acetylcholinesterase, oxidative stress and TLR4-NF-kB(p65)-NLRP3 pathways.
34339751	8	20	theme	Aspartate	1620:1628	arg1	caspase-1					1642:1650	caspase-1	1642:1650	caspase-1	1642:1650	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	8	20	theme	Aspartate	1620:1628	arg1	Protease-1					1630:1639	Cysteinyl Aspartate Protease-1	1610:1639	Cysteinyl Aspartate Protease-1 (caspase-1)	1610:1651	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	5	21	theme	72h	966:968	arg1	period					970:975	a 72h period	964:975	a 72h period	964:975	In the present study, chrysin was successfully formulated as transfersomal lipid vesicles and chitosan composite vesicles (CCV) exhibiting a nanometric size range, high drug entrapment efficiency, and controlled release over a 72h period.
34339751	4	22	theme	Chrysin	549:555	arg1	dihydroxyflavone-					562:578	Chrysin -5,7 dihydroxyflavone-	549:578	Chrysin -5,7 dihydroxyflavone-	549:578	Chrysin -5,7 dihydroxyflavone- has promising antioxidant, anti-inflammatory and anticancer properties, but suffers low bioavailability owing to its poor solubility and extensive metabolism.
34339751	8	23	theme	p65	1468:1470	arg1	subunit					1472:1478	p65 subunit	1468:1478	p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells	1468:1544	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	0	24	theme	cognitive	94:102	arg1	impairment					104:113	doxorubicin-induced cognitive impairment	74:113	doxorubicin-induced cognitive impairment in rats	74:121	Nose-to-brain delivery of chrysin transfersomal and composite vesicles in doxorubicin-induced cognitive impairment in rats: Insights on formulation, oxidative stress and TLR4/NF-kB/NLRP3 pathways.
34339751	6	25	theme	memory	1098:1103	arg1	impairment					1105:1114	doxorubicin-induced memory impairment	1078:1114	doxorubicin-induced memory impairment	1078:1114	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg improved doxorubicin-induced memory impairment in rats evidenced by behavioral testing, inhibition of acetylcholinesterase activity and oxidative stress markers; catalase, reduced glutathione, lipid peroxidation and hydrogen peroxide.
34339751	9	26	theme	acetylcholinesterase	1949:1968	arg1	pathways					2014:2021	acetylcholinesterase, oxidative stress and TLR4-NF-kB(p65)-NLRP3 pathways	1949:2021	acetylcholinesterase, oxidative stress and TLR4-NF-kB(p65)-NLRP3 pathways	1949:2021	Collectively, our findings suggest the enhanced therapeutic potential of chrysin when formulated as transfersomes and CCV against chemotherapy-induced chemobrain via hindering acetylcholinesterase, oxidative stress and TLR4-NF-kB(p65)-NLRP3 pathways.
34339751	8	27	theme	activated	1528:1536	arg1	cells					1540:1544	activated B cells	1528:1544	activated B cells	1528:1544	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	7	28	theme	expression	1332:1341	arg1	apoptosis					1354:1362	caspase-3 expression inhibiting apoptosis	1322:1362	caspase-3 expression inhibiting apoptosis	1322:1362	This could reduce caspase-3 expression inhibiting apoptosis.
34339751	9	29	theme	oxidative	1971:1979	arg1	stress					1981:1986	oxidative stress	1971:1986	oxidative stress	1971:1986	Collectively, our findings suggest the enhanced therapeutic potential of chrysin when formulated as transfersomes and CCV against chemotherapy-induced chemobrain via hindering acetylcholinesterase, oxidative stress and TLR4-NF-kB(p65)-NLRP3 pathways.
34339751	8	30	theme	Interleukin-1β	1657:1670	arg1	expression					1688:1697	Interleukin-1β (IL-1β) protein expression	1657:1697	Interleukin-1β (IL-1β) protein expression	1657:1697	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	8	31	theme	factor	1491:1496	arg1	kappa-light-chain-enhancer					1498:1523	nuclear factor kappa-light-chain-enhancer	1483:1523	nuclear factor kappa-light-chain-enhancer of activated B cells	1483:1544	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	3	32	theme	cancer	476:481	arg1	patients					483:490	cancer patients	476:490	cancer patients	476:490	However, it is reported to precipitate cognitive impairment in cancer patients via inducing oxidative stress and inflammatory response.
34339751	5	33	theme	chitosan	833:840	arg1	chrysin					761:767	chrysin	761:767	chrysin	761:767	In the present study, chrysin was successfully formulated as transfersomal lipid vesicles and chitosan composite vesicles (CCV) exhibiting a nanometric size range, high drug entrapment efficiency, and controlled release over a 72h period.
34339751	5	33	theme	chitosan	833:840	arg1	vesicles					852:859	chitosan composite vesicles	833:859	chitosan composite vesicles	833:859	In the present study, chrysin was successfully formulated as transfersomal lipid vesicles and chitosan composite vesicles (CCV) exhibiting a nanometric size range, high drug entrapment efficiency, and controlled release over a 72h period.
34339751	6	34	theme	activity	1192:1199	arg1	testing					1148:1154	behavioral testing	1137:1154	behavioral testing	1137:1154	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg improved doxorubicin-induced memory impairment in rats evidenced by behavioral testing, inhibition of acetylcholinesterase activity and oxidative stress markers; catalase, reduced glutathione, lipid peroxidation and hydrogen peroxide.
34339751	6	34	theme	activity	1192:1199	arg1	glutathione					1249:1259	reduced glutathione	1241:1259	reduced glutathione	1241:1259	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg improved doxorubicin-induced memory impairment in rats evidenced by behavioral testing, inhibition of acetylcholinesterase activity and oxidative stress markers; catalase, reduced glutathione, lipid peroxidation and hydrogen peroxide.
34339751	6	34	theme	activity	1192:1199	arg1	peroxide					1294:1301	hydrogen peroxide	1285:1301	hydrogen peroxide	1285:1301	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg improved doxorubicin-induced memory impairment in rats evidenced by behavioral testing, inhibition of acetylcholinesterase activity and oxidative stress markers; catalase, reduced glutathione, lipid peroxidation and hydrogen peroxide.
34339751	6	34	theme	activity	1192:1199	arg1	inhibition					1157:1166	inhibition	1157:1166	inhibition of acetylcholinesterase activity and oxidative stress markers	1157:1228	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg improved doxorubicin-induced memory impairment in rats evidenced by behavioral testing, inhibition of acetylcholinesterase activity and oxidative stress markers; catalase, reduced glutathione, lipid peroxidation and hydrogen peroxide.
34339751	6	34	theme	activity	1192:1199	arg1	peroxidation					1268:1279	lipid peroxidation	1262:1279	lipid peroxidation	1262:1279	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg improved doxorubicin-induced memory impairment in rats evidenced by behavioral testing, inhibition of acetylcholinesterase activity and oxidative stress markers; catalase, reduced glutathione, lipid peroxidation and hydrogen peroxide.
34339751	6	34	theme	activity	1192:1199	arg1	catalase					1231:1238	catalase	1231:1238	catalase	1231:1238	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg improved doxorubicin-induced memory impairment in rats evidenced by behavioral testing, inhibition of acetylcholinesterase activity and oxidative stress markers; catalase, reduced glutathione, lipid peroxidation and hydrogen peroxide.
34339751	0	35	theme	doxorubicin-induced	74:92	arg1	impairment					104:113	doxorubicin-induced cognitive impairment	74:113	doxorubicin-induced cognitive impairment in rats	74:121	Nose-to-brain delivery of chrysin transfersomal and composite vesicles in doxorubicin-induced cognitive impairment in rats: Insights on formulation, oxidative stress and TLR4/NF-kB/NLRP3 pathways.
34339751	0	36	theme	Nose-to-brain	0:12	arg1	delivery					14:21	Nose-to-brain delivery	0:21	Nose-to-brain delivery of chrysin transfersomal and composite vesicles in doxorubicin-induced cognitive impairment in rats: Insights on formulation, oxidative stress and TLR4/NF-kB/NLRP3 pathways.	0:195	Nose-to-brain delivery of chrysin transfersomal and composite vesicles in doxorubicin-induced cognitive impairment in rats: Insights on formulation, oxidative stress and TLR4/NF-kB/NLRP3 pathways.
34339751	8	37	theme	protein	1680:1686	arg1	expression					1688:1697	Interleukin-1β (IL-1β) protein expression	1657:1697	Interleukin-1β (IL-1β) protein expression	1657:1697	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	6	38	from	dose	1051:1054	arg1	administration					989:1002	Intranasal administration	978:1002	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg	978:1067	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg improved doxorubicin-induced memory impairment in rats evidenced by behavioral testing, inhibition of acetylcholinesterase activity and oxidative stress markers; catalase, reduced glutathione, lipid peroxidation and hydrogen peroxide.
34339751	8	39	theme	B	1538:1538	arg1	cells					1540:1544	activated B cells	1528:1544	activated B cells	1528:1544	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	3	40	theme	oxidative	505:513	arg1	stress					515:520	oxidative stress	505:520	oxidative stress	505:520	However, it is reported to precipitate cognitive impairment in cancer patients via inducing oxidative stress and inflammatory response.
34339751	5	41	theme	high	903:906	arg1	efficiency					924:933	high drug entrapment efficiency	903:933	high drug entrapment efficiency	903:933	In the present study, chrysin was successfully formulated as transfersomal lipid vesicles and chitosan composite vesicles (CCV) exhibiting a nanometric size range, high drug entrapment efficiency, and controlled release over a 72h period.
34339751	0	42	theme	transfersomal	34:46	arg1	vesicles					62:69	chrysin transfersomal and composite vesicles	26:69	chrysin transfersomal and composite vesicles	26:69	Nose-to-brain delivery of chrysin transfersomal and composite vesicles in doxorubicin-induced cognitive impairment in rats: Insights on formulation, oxidative stress and TLR4/NF-kB/NLRP3 pathways.
34339751	9	43	theme	-NLRP3	2007:2012	arg1	pathways					2014:2021	acetylcholinesterase, oxidative stress and TLR4-NF-kB(p65)-NLRP3 pathways	1949:2021	acetylcholinesterase, oxidative stress and TLR4-NF-kB(p65)-NLRP3 pathways	1949:2021	Collectively, our findings suggest the enhanced therapeutic potential of chrysin when formulated as transfersomes and CCV against chemotherapy-induced chemobrain via hindering acetylcholinesterase, oxidative stress and TLR4-NF-kB(p65)-NLRP3 pathways.
34339751	6	44	theme	behavioral	1137:1146	arg1	testing					1148:1154	behavioral testing	1137:1154	behavioral testing	1137:1154	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg improved doxorubicin-induced memory impairment in rats evidenced by behavioral testing, inhibition of acetylcholinesterase activity and oxidative stress markers; catalase, reduced glutathione, lipid peroxidation and hydrogen peroxide.
34339751	4	45	theme	antioxidant	594:604	arg1	properties					640:649	promising antioxidant, anti-inflammatory and anticancer properties	584:649	promising antioxidant, anti-inflammatory and anticancer properties	584:649	Chrysin -5,7 dihydroxyflavone- has promising antioxidant, anti-inflammatory and anticancer properties, but suffers low bioavailability owing to its poor solubility and extensive metabolism.
34339751	5	46	theme	nanometric	880:889	arg1	range					896:900	a nanometric size range	878:900	a nanometric size range	878:900	In the present study, chrysin was successfully formulated as transfersomal lipid vesicles and chitosan composite vesicles (CCV) exhibiting a nanometric size range, high drug entrapment efficiency, and controlled release over a 72h period.
34339751	6	47	theme	reduced	1241:1247	arg1	glutathione					1249:1259	reduced glutathione	1241:1259	reduced glutathione	1241:1259	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg improved doxorubicin-induced memory impairment in rats evidenced by behavioral testing, inhibition of acetylcholinesterase activity and oxidative stress markers; catalase, reduced glutathione, lipid peroxidation and hydrogen peroxide.
34339751	8	48	theme	3	1749:1749	arg1	inflammasome					1759:1770	3 (NLRP3) inflammasome	1749:1770	3 (NLRP3) inflammasome	1749:1770	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	5	49	theme	controlled	940:949	arg1	release					951:957	controlled release	940:957	controlled release	940:957	In the present study, chrysin was successfully formulated as transfersomal lipid vesicles and chitosan composite vesicles (CCV) exhibiting a nanometric size range, high drug entrapment efficiency, and controlled release over a 72h period.
34339751	6	50	theme	optimized	1007:1015	arg1	formulations					1025:1036	optimized chrysin formulations	1007:1036	optimized chrysin formulations	1007:1036	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg improved doxorubicin-induced memory impairment in rats evidenced by behavioral testing, inhibition of acetylcholinesterase activity and oxidative stress markers; catalase, reduced glutathione, lipid peroxidation and hydrogen peroxide.
34339751	4	51	theme	promising	584:592	arg1	properties					640:649	promising antioxidant, anti-inflammatory and anticancer properties	584:649	promising antioxidant, anti-inflammatory and anticancer properties	584:649	Chrysin -5,7 dihydroxyflavone- has promising antioxidant, anti-inflammatory and anticancer properties, but suffers low bioavailability owing to its poor solubility and extensive metabolism.
34339751	2	52	theme	other	396:400	arg1	neoplasms					402:410	other neoplasms	396:410	other neoplasms	396:410	Doxorubicin -topoisomerase II inhibitor- is widely used in breast cancer, hematological cancers and other neoplasms.
34339751	6	53	theme	oxidative	1205:1213	arg1	stress					1215:1220	oxidative stress	1205:1220	oxidative stress	1205:1220	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg improved doxorubicin-induced memory impairment in rats evidenced by behavioral testing, inhibition of acetylcholinesterase activity and oxidative stress markers; catalase, reduced glutathione, lipid peroxidation and hydrogen peroxide.
34339751	8	54	theme	Nod-like	1714:1721	arg1	pyrin					1732:1736	Nod-like receptor pyrin	1714:1736	Nod-like receptor pyrin containing 3 (NLRP3) inflammasome	1714:1770	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	4	55	theme	low	664:666	arg1	bioavailability					668:682	low bioavailability	664:682	low bioavailability owing to its poor solubility and extensive metabolism	664:736	Chrysin -5,7 dihydroxyflavone- has promising antioxidant, anti-inflammatory and anticancer properties, but suffers low bioavailability owing to its poor solubility and extensive metabolism.
34339751	0	56	theme	composite	52:60	arg1	vesicles					62:69	chrysin transfersomal and composite vesicles	26:69	chrysin transfersomal and composite vesicles	26:69	Nose-to-brain delivery of chrysin transfersomal and composite vesicles in doxorubicin-induced cognitive impairment in rats: Insights on formulation, oxidative stress and TLR4/NF-kB/NLRP3 pathways.
34339751	9	57	theme	therapeutic	1821:1831	arg1	potential					1833:1841	the enhanced therapeutic potential	1808:1841	the enhanced therapeutic potential of chrysin	1808:1852	Collectively, our findings suggest the enhanced therapeutic potential of chrysin when formulated as transfersomes and CCV against chemotherapy-induced chemobrain via hindering acetylcholinesterase, oxidative stress and TLR4-NF-kB(p65)-NLRP3 pathways.
34339751	6	58	theme	hydrogen	1285:1292	arg1	peroxide					1294:1301	hydrogen peroxide	1285:1301	hydrogen peroxide	1285:1301	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg improved doxorubicin-induced memory impairment in rats evidenced by behavioral testing, inhibition of acetylcholinesterase activity and oxidative stress markers; catalase, reduced glutathione, lipid peroxidation and hydrogen peroxide.
34339751	2	59	theme	breast	355:360	arg1	cancer					362:367	breast cancer	355:367	breast cancer	355:367	Doxorubicin -topoisomerase II inhibitor- is widely used in breast cancer, hematological cancers and other neoplasms.
34339751	1	60	theme	cancer	202:207	arg1	survivors					209:217	Many cancer survivors	197:217	Many cancer survivors	197:217	Many cancer survivors suffer from chemotherapy-induced cognitive impairment known as 'Chemobrain'.
34339751	6	61	theme	0.5 mg/kg	1059:1067	arg1	dose					1051:1054	a reduced dose	1041:1054	a reduced dose of 0.5 mg/kg	1041:1067	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg improved doxorubicin-induced memory impairment in rats evidenced by behavioral testing, inhibition of acetylcholinesterase activity and oxidative stress markers; catalase, reduced glutathione, lipid peroxidation and hydrogen peroxide.
34339751	5	62	theme	drug	908:911	arg1	efficiency					924:933	high drug entrapment efficiency	903:933	high drug entrapment efficiency	903:933	In the present study, chrysin was successfully formulated as transfersomal lipid vesicles and chitosan composite vesicles (CCV) exhibiting a nanometric size range, high drug entrapment efficiency, and controlled release over a 72h period.
34339751	0	63	from	impairment	104:113	arg1	rats					118:121	rats	118:121	rats	118:121	Nose-to-brain delivery of chrysin transfersomal and composite vesicles in doxorubicin-induced cognitive impairment in rats: Insights on formulation, oxidative stress and TLR4/NF-kB/NLRP3 pathways.
34339751	0	64	theme	oxidative	149:157	arg1	stress					159:164	oxidative stress	149:164	oxidative stress	149:164	Nose-to-brain delivery of chrysin transfersomal and composite vesicles in doxorubicin-induced cognitive impairment in rats: Insights on formulation, oxidative stress and TLR4/NF-kB/NLRP3 pathways.
34339751	1	65	theme	chemotherapy-induced	231:250	arg1	impairment					262:271	chemotherapy-induced cognitive impairment	231:271	chemotherapy-induced cognitive impairment known as 'Chemobrain'	231:293	Many cancer survivors suffer from chemotherapy-induced cognitive impairment known as 'Chemobrain'.
34339751	4	66	theme	extensive	717:725	arg1	metabolism					727:736	extensive metabolism	717:736	extensive metabolism	717:736	Chrysin -5,7 dihydroxyflavone- has promising antioxidant, anti-inflammatory and anticancer properties, but suffers low bioavailability owing to its poor solubility and extensive metabolism.
34339751	7	67	theme	caspase-3	1322:1330	arg1	apoptosis					1354:1362	caspase-3 expression inhibiting apoptosis	1322:1362	caspase-3 expression inhibiting apoptosis	1322:1362	This could reduce caspase-3 expression inhibiting apoptosis.
34339751	6	68	theme	formulations	1025:1036	arg1	administration					989:1002	Intranasal administration	978:1002	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg	978:1067	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg improved doxorubicin-induced memory impairment in rats evidenced by behavioral testing, inhibition of acetylcholinesterase activity and oxidative stress markers; catalase, reduced glutathione, lipid peroxidation and hydrogen peroxide.
34339751	8	69	theme	Cysteinyl	1610:1618	arg1	caspase-1					1642:1650	caspase-1	1642:1650	caspase-1	1642:1650	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	8	69	theme	Cysteinyl	1610:1618	arg1	Protease-1					1630:1639	Cysteinyl Aspartate Protease-1	1610:1639	Cysteinyl Aspartate Protease-1 (caspase-1)	1610:1651	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	8	70	theme	Tol-like	1437:1444	arg1	NF-kB					1547:1551	NF-kB	1547:1551	NF-kB	1547:1551	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	8	70	theme	Tol-like	1437:1444	arg1	receptor					1446:1453	doxorubicin-induced Tol-like receptor 4	1417:1455	doxorubicin-induced Tol-like receptor 4 (TLR4)	1417:1462	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	8	70	theme	Tol-like	1437:1444	arg1	TLR4					1458:1461	TLR4	1458:1461	TLR4	1458:1461	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	2	71	theme	Doxorubicin	296:306	arg1	inhibitor-					326:335	Doxorubicin -topoisomerase II inhibitor-	296:335	Doxorubicin -topoisomerase II inhibitor-	296:335	Doxorubicin -topoisomerase II inhibitor- is widely used in breast cancer, hematological cancers and other neoplasms.
34339751	5	72	theme	composite	842:850	arg1	chrysin					761:767	chrysin	761:767	chrysin	761:767	In the present study, chrysin was successfully formulated as transfersomal lipid vesicles and chitosan composite vesicles (CCV) exhibiting a nanometric size range, high drug entrapment efficiency, and controlled release over a 72h period.
34339751	5	72	theme	composite	842:850	arg1	vesicles					852:859	chitosan composite vesicles	833:859	chitosan composite vesicles	833:859	In the present study, chrysin was successfully formulated as transfersomal lipid vesicles and chitosan composite vesicles (CCV) exhibiting a nanometric size range, high drug entrapment efficiency, and controlled release over a 72h period.
34339751	7	73	theme	inhibiting	1343:1352	arg1	apoptosis					1354:1362	caspase-3 expression inhibiting apoptosis	1322:1362	caspase-3 expression inhibiting apoptosis	1322:1362	This could reduce caspase-3 expression inhibiting apoptosis.
34339751	0	74	from	delivery	14:21	arg1	impairment					104:113	doxorubicin-induced cognitive impairment	74:113	doxorubicin-induced cognitive impairment in rats	74:121	Nose-to-brain delivery of chrysin transfersomal and composite vesicles in doxorubicin-induced cognitive impairment in rats: Insights on formulation, oxidative stress and TLR4/NF-kB/NLRP3 pathways.
34339751	5	75	theme	lipid	814:818	arg1	chrysin					761:767	chrysin	761:767	chrysin	761:767	In the present study, chrysin was successfully formulated as transfersomal lipid vesicles and chitosan composite vesicles (CCV) exhibiting a nanometric size range, high drug entrapment efficiency, and controlled release over a 72h period.
34339751	5	75	theme	lipid	814:818	arg1	vesicles					820:827	transfersomal lipid vesicles	800:827	transfersomal lipid vesicles	800:827	In the present study, chrysin was successfully formulated as transfersomal lipid vesicles and chitosan composite vesicles (CCV) exhibiting a nanometric size range, high drug entrapment efficiency, and controlled release over a 72h period.
34339751	5	75	theme	lipid	814:818	arg1	CCV					862:864	CCV	862:864	CCV	862:864	In the present study, chrysin was successfully formulated as transfersomal lipid vesicles and chitosan composite vesicles (CCV) exhibiting a nanometric size range, high drug entrapment efficiency, and controlled release over a 72h period.
34339751	6	76	theme	doxorubicin-induced	1078:1096	arg1	impairment					1105:1114	doxorubicin-induced memory impairment	1078:1114	doxorubicin-induced memory impairment	1078:1114	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg improved doxorubicin-induced memory impairment in rats evidenced by behavioral testing, inhibition of acetylcholinesterase activity and oxidative stress markers; catalase, reduced glutathione, lipid peroxidation and hydrogen peroxide.
34339751	8	77	theme	subunit	1472:1478	arg1	expression					1562:1571	doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression	1417:1571	doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome	1417:1770	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	9	78	theme	stress	1981:1986	arg1	pathways					2014:2021	acetylcholinesterase, oxidative stress and TLR4-NF-kB(p65)-NLRP3 pathways	1949:2021	acetylcholinesterase, oxidative stress and TLR4-NF-kB(p65)-NLRP3 pathways	1949:2021	Collectively, our findings suggest the enhanced therapeutic potential of chrysin when formulated as transfersomes and CCV against chemotherapy-induced chemobrain via hindering acetylcholinesterase, oxidative stress and TLR4-NF-kB(p65)-NLRP3 pathways.
34339751	8	79	theme	nuclear	1483:1489	arg1	kappa-light-chain-enhancer					1498:1523	nuclear factor kappa-light-chain-enhancer	1483:1523	nuclear factor kappa-light-chain-enhancer of activated B cells	1483:1544	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	5	80	theme	size	891:894	arg1	range					896:900	a nanometric size range	878:900	a nanometric size range	878:900	In the present study, chrysin was successfully formulated as transfersomal lipid vesicles and chitosan composite vesicles (CCV) exhibiting a nanometric size range, high drug entrapment efficiency, and controlled release over a 72h period.
34339751	6	81	theme	acetylcholinesterase	1171:1190	arg1	activity					1192:1199	acetylcholinesterase activity	1171:1199	acetylcholinesterase activity	1171:1199	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg improved doxorubicin-induced memory impairment in rats evidenced by behavioral testing, inhibition of acetylcholinesterase activity and oxidative stress markers; catalase, reduced glutathione, lipid peroxidation and hydrogen peroxide.
34339751	9	82	theme	TLR4-NF-kB	1992:2001	arg1	pathways					2014:2021	acetylcholinesterase, oxidative stress and TLR4-NF-kB(p65)-NLRP3 pathways	1949:2021	acetylcholinesterase, oxidative stress and TLR4-NF-kB(p65)-NLRP3 pathways	1949:2021	Collectively, our findings suggest the enhanced therapeutic potential of chrysin when formulated as transfersomes and CCV against chemotherapy-induced chemobrain via hindering acetylcholinesterase, oxidative stress and TLR4-NF-kB(p65)-NLRP3 pathways.
34339751	4	83	theme	anticancer	629:638	arg1	properties					640:649	promising antioxidant, anti-inflammatory and anticancer properties	584:649	promising antioxidant, anti-inflammatory and anticancer properties	584:649	Chrysin -5,7 dihydroxyflavone- has promising antioxidant, anti-inflammatory and anticancer properties, but suffers low bioavailability owing to its poor solubility and extensive metabolism.
34339751	8	84	theme	kappa-light-chain-enhancer	1498:1523	arg1	NF-kB					1547:1551	NF-kB	1547:1551	NF-kB	1547:1551	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	8	84	theme	kappa-light-chain-enhancer	1498:1523	arg1	subunit					1472:1478	p65 subunit	1468:1478	p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells	1468:1544	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	8	84	theme	kappa-light-chain-enhancer	1498:1523	arg1	receptor					1446:1453	doxorubicin-induced Tol-like receptor 4	1417:1455	doxorubicin-induced Tol-like receptor 4 (TLR4)	1417:1462	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	8	84	theme	kappa-light-chain-enhancer	1498:1523	arg1	TLR4					1458:1461	TLR4	1458:1461	TLR4	1458:1461	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	0	85	theme	chrysin	26:32	arg1	vesicles					62:69	chrysin transfersomal and composite vesicles	26:69	chrysin transfersomal and composite vesicles	26:69	Nose-to-brain delivery of chrysin transfersomal and composite vesicles in doxorubicin-induced cognitive impairment in rats: Insights on formulation, oxidative stress and TLR4/NF-kB/NLRP3 pathways.
34339751	6	86	dep	activity	1192:1199	arg1	markers					1222:1228	markers	1222:1228	markers	1222:1228	Intranasal administration of optimized chrysin formulations at a reduced dose of 0.5 mg/kg improved doxorubicin-induced memory impairment in rats evidenced by behavioral testing, inhibition of acetylcholinesterase activity and oxidative stress markers; catalase, reduced glutathione, lipid peroxidation and hydrogen peroxide.
34339751	3	87	theme	cognitive	452:460	arg1	impairment					462:471	cognitive impairment	452:471	cognitive impairment in cancer patients	452:490	However, it is reported to precipitate cognitive impairment in cancer patients via inducing oxidative stress and inflammatory response.
34339751	8	88	theme	receptor	1723:1730	arg1	pyrin					1732:1736	Nod-like receptor pyrin	1714:1736	Nod-like receptor pyrin containing 3 (NLRP3) inflammasome	1714:1770	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	8	89	theme	cells	1540:1544	arg1	kappa-light-chain-enhancer					1498:1523	nuclear factor kappa-light-chain-enhancer	1483:1523	nuclear factor kappa-light-chain-enhancer of activated B cells	1483:1544	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	0	90	theme	vesicles	62:69	arg1	delivery					14:21	Nose-to-brain delivery	0:21	Nose-to-brain delivery of chrysin transfersomal and composite vesicles in doxorubicin-induced cognitive impairment in rats: Insights on formulation, oxidative stress and TLR4/NF-kB/NLRP3 pathways.	0:195	Nose-to-brain delivery of chrysin transfersomal and composite vesicles in doxorubicin-induced cognitive impairment in rats: Insights on formulation, oxidative stress and TLR4/NF-kB/NLRP3 pathways.
34339751	4	91	theme	anti-inflammatory	607:623	arg1	properties					640:649	promising antioxidant, anti-inflammatory and anticancer properties	584:649	promising antioxidant, anti-inflammatory and anticancer properties	584:649	Chrysin -5,7 dihydroxyflavone- has promising antioxidant, anti-inflammatory and anticancer properties, but suffers low bioavailability owing to its poor solubility and extensive metabolism.
34339751	2	92	theme	hematological	370:382	arg1	cancers					384:390	hematological cancers	370:390	hematological cancers	370:390	Doxorubicin -topoisomerase II inhibitor- is widely used in breast cancer, hematological cancers and other neoplasms.
34339751	2	93	used	used	347:350	arg2	inhibitor-					326:335	Doxorubicin -topoisomerase II inhibitor-	296:335	Doxorubicin -topoisomerase II inhibitor-	296:335	Doxorubicin -topoisomerase II inhibitor- is widely used in breast cancer, hematological cancers and other neoplasms.
34339751	8	94	theme	protein	1554:1560	arg1	expression					1562:1571	doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression	1417:1571	doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome	1417:1770	Moreover, chrysin formulations were able to inhibit doxorubicin-induced Tol-like receptor 4 (TLR4) and p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) protein expression which in turn, reduced procaspase-1, Cysteinyl Aspartate Protease-1 (caspase-1) and Interleukin-1β (IL-1β) protein expression via inhibiting Nod-like receptor pyrin containing 3 (NLRP3) inflammasome.
34339751	3	95	from	impairment	462:471	arg1	patients					483:490	cancer patients	476:490	cancer patients	476:490	However, it is reported to precipitate cognitive impairment in cancer patients via inducing oxidative stress and inflammatory response.
34339751	3	96	theme	inflammatory	526:537	arg1	response					539:546	inflammatory response	526:546	inflammatory response	526:546	However, it is reported to precipitate cognitive impairment in cancer patients via inducing oxidative stress and inflammatory response.
34339751	0	97	dep	delivery	14:21	arg1	Insights					124:131	Insights	124:131	Nose-to-brain delivery of chrysin transfersomal and composite vesicles in doxorubicin-induced cognitive impairment in rats: Insights on formulation, oxidative stress and TLR4/NF-kB/NLRP3 pathways.	0:195	Nose-to-brain delivery of chrysin transfersomal and composite vesicles in doxorubicin-induced cognitive impairment in rats: Insights on formulation, oxidative stress and TLR4/NF-kB/NLRP3 pathways.
34339751	4	98	theme	poor	697:700	arg1	solubility					702:711	its poor solubility	693:711	its poor solubility	693:711	Chrysin -5,7 dihydroxyflavone- has promising antioxidant, anti-inflammatory and anticancer properties, but suffers low bioavailability owing to its poor solubility and extensive metabolism.
34339751	4	99	contain	has	580:582	arg2	properties					640:649	promising antioxidant, anti-inflammatory and anticancer properties	584:649	promising antioxidant, anti-inflammatory and anticancer properties	584:649	Chrysin -5,7 dihydroxyflavone- has promising antioxidant, anti-inflammatory and anticancer properties, but suffers low bioavailability owing to its poor solubility and extensive metabolism.
34339751	4	99	contain	has	580:582	arg1	dihydroxyflavone-					562:578	Chrysin -5,7 dihydroxyflavone-	549:578	Chrysin -5,7 dihydroxyflavone-	549:578	Chrysin -5,7 dihydroxyflavone- has promising antioxidant, anti-inflammatory and anticancer properties, but suffers low bioavailability owing to its poor solubility and extensive metabolism.
32433917	8	0	theme	in	890:891	arg1	healing					904:910	The in vivo wound healing	886:910	The in vivo wound healing	886:910	The in vivo wound healing was evaluated using animal wound infection model of seawater immersion, and it was observed that the prepared composite scaffolds accelerated wound healing, reepithelialization, collagen deposition.
32433917	5	1	theme	proper	606:611	arg1	porosity					613:620	proper porosity	606:620	proper porosity	606:620	From in vitro characterization results, CS/GEL/GMs-CIP had high water absorption ability, proper porosity, satisfactory fracture resistance, and flexibility.
32433917	10	2	theme	trauma	1482:1487	arg1	infections					1495:1504	open trauma wound infections	1477:1504	open trauma wound infections	1477:1504	In summary, we believe that CS/GEL/GMs-CIP composite scaffold may serve as a promising multifunctional dressing for healing with open trauma wound infections and wound with seawater immersion.
32433917	9	3	theme	factor	1295:1300	arg1	expression					1264:1273	expression	1264:1273	expression	1264:1273	Further analysis of wound tissue indicated that the expression of anti-inflammatory factor (TGF-β1) was up-regulated, but the serum endotoxin levels and expression of pro-inflammatory factor (TNF-a, IL-6, and IL-1β) were down-regulated.
32433917	9	3	theme	factor	1295:1300	arg1	levels					1253:1258	the serum endotoxin levels	1233:1258	the serum endotoxin levels	1233:1258	Further analysis of wound tissue indicated that the expression of anti-inflammatory factor (TGF-β1) was up-regulated, but the serum endotoxin levels and expression of pro-inflammatory factor (TNF-a, IL-6, and IL-1β) were down-regulated.
32433917	10	4	with	healing	1464:1470	arg1	infections					1495:1504	open trauma wound infections	1477:1504	open trauma wound infections	1477:1504	In summary, we believe that CS/GEL/GMs-CIP composite scaffold may serve as a promising multifunctional dressing for healing with open trauma wound infections and wound with seawater immersion.
32433917	10	4	with	healing	1464:1470	arg1	wound					1510:1514	wound	1510:1514	wound with seawater immersion	1510:1538	In summary, we believe that CS/GEL/GMs-CIP composite scaffold may serve as a promising multifunctional dressing for healing with open trauma wound infections and wound with seawater immersion.
32433917	7	5	contain	had	809:811	arg2	effect					838:843	a significant inhibitory effect	813:843	a significant inhibitory effect	813:843	Antibacterial experiments confirmed that CS/GEL/GMs-CIP had a significant inhibitory effect on E. coli, S. aureus and P. aeruginosa.
32433917	7	5	contain	had	809:811	arg1	CS/GEL/GMs-CIP					794:807	CS/GEL/GMs-CIP	794:807	CS/GEL/GMs-CIP	794:807	Antibacterial experiments confirmed that CS/GEL/GMs-CIP had a significant inhibitory effect on E. coli, S. aureus and P. aeruginosa.
32433917	10	6	theme	wound	1489:1493	arg1	infections					1495:1504	open trauma wound infections	1477:1504	open trauma wound infections	1477:1504	In summary, we believe that CS/GEL/GMs-CIP composite scaffold may serve as a promising multifunctional dressing for healing with open trauma wound infections and wound with seawater immersion.
32433917	9	7	theme	serum	1237:1241	arg1	levels					1253:1258	the serum endotoxin levels	1233:1258	the serum endotoxin levels	1233:1258	Further analysis of wound tissue indicated that the expression of anti-inflammatory factor (TGF-β1) was up-regulated, but the serum endotoxin levels and expression of pro-inflammatory factor (TNF-a, IL-6, and IL-1β) were down-regulated.
32433917	8	8	theme	wound	898:902	arg1	healing					904:910	The in vivo wound healing	886:910	The in vivo wound healing	886:910	The in vivo wound healing was evaluated using animal wound infection model of seawater immersion, and it was observed that the prepared composite scaffolds accelerated wound healing, reepithelialization, collagen deposition.
32433917	4	9	dep	microspheres	447:458	arg1	GMs-CIP					497:503	GMs-CIP	497:503	GMs-CIP	497:503	The chitosan (CS) and gelatin (GEL) were cross-linked by genipin as matrix, and then gelatin microspheres loading ciprofloxacin hydrochloride (GMs-CIP) were add.
32433917	4	9	dep	microspheres	447:458	arg1	hydrochloride					482:494	loading ciprofloxacin hydrochloride	460:494	gelatin microspheres loading ciprofloxacin hydrochloride (GMs-CIP)	439:504	The chitosan (CS) and gelatin (GEL) were cross-linked by genipin as matrix, and then gelatin microspheres loading ciprofloxacin hydrochloride (GMs-CIP) were add.
32433917	9	10	theme	factor	1195:1200	arg1	expression					1163:1172	the expression	1159:1172	the expression of anti-inflammatory factor (TGF-β1)	1159:1209	Further analysis of wound tissue indicated that the expression of anti-inflammatory factor (TGF-β1) was up-regulated, but the serum endotoxin levels and expression of pro-inflammatory factor (TNF-a, IL-6, and IL-1β) were down-regulated.
32433917	8	11	theme	collagen	1090:1097	arg1	deposition					1099:1108	collagen deposition	1090:1108	collagen deposition	1090:1108	The in vivo wound healing was evaluated using animal wound infection model of seawater immersion, and it was observed that the prepared composite scaffolds accelerated wound healing, reepithelialization, collagen deposition.
32433917	8	11	theme	collagen	1090:1097	arg1	healing					1060:1066	wound healing	1054:1066	wound healing	1054:1066	The in vivo wound healing was evaluated using animal wound infection model of seawater immersion, and it was observed that the prepared composite scaffolds accelerated wound healing, reepithelialization, collagen deposition.
32433917	9	12	theme	endotoxin	1243:1251	arg1	levels					1253:1258	the serum endotoxin levels	1233:1258	the serum endotoxin levels	1233:1258	Further analysis of wound tissue indicated that the expression of anti-inflammatory factor (TGF-β1) was up-regulated, but the serum endotoxin levels and expression of pro-inflammatory factor (TNF-a, IL-6, and IL-1β) were down-regulated.
32433917	6	13	theme	composite	702:710	arg1	scaffold					712:719	CS/GEL/GMs-CIP composite scaffold	687:719	CS/GEL/GMs-CIP composite scaffold	687:719	Furthermore, CS/GEL/GMs-CIP composite scaffold had excellent biocompatibility.
32433917	8	14	theme	prepared	1013:1020	arg1	scaffolds					1032:1040	the prepared composite scaffolds	1009:1040	the prepared composite scaffolds	1009:1040	The in vivo wound healing was evaluated using animal wound infection model of seawater immersion, and it was observed that the prepared composite scaffolds accelerated wound healing, reepithelialization, collagen deposition.
32433917	10	15	with	infections	1495:1504	arg1	immersion					1530:1538	seawater immersion	1521:1538	seawater immersion	1521:1538	In summary, we believe that CS/GEL/GMs-CIP composite scaffold may serve as a promising multifunctional dressing for healing with open trauma wound infections and wound with seawater immersion.
32433917	9	16	theme	wound	1131:1135	arg1	tissue					1137:1142	wound tissue	1131:1142	wound tissue	1131:1142	Further analysis of wound tissue indicated that the expression of anti-inflammatory factor (TGF-β1) was up-regulated, but the serum endotoxin levels and expression of pro-inflammatory factor (TNF-a, IL-6, and IL-1β) were down-regulated.
32433917	5	17	theme	fracture	636:643	arg1	resistance					645:654	satisfactory fracture resistance	623:654	satisfactory fracture resistance	623:654	From in vitro characterization results, CS/GEL/GMs-CIP had high water absorption ability, proper porosity, satisfactory fracture resistance, and flexibility.
32433917	5	18	theme	satisfactory	623:634	arg1	resistance					645:654	satisfactory fracture resistance	623:654	satisfactory fracture resistance	623:654	From in vitro characterization results, CS/GEL/GMs-CIP had high water absorption ability, proper porosity, satisfactory fracture resistance, and flexibility.
32433917	9	19	theme	tissue	1137:1142	arg1	analysis					1119:1126	Further analysis	1111:1126	Further analysis of wound tissue	1111:1142	Further analysis of wound tissue indicated that the expression of anti-inflammatory factor (TGF-β1) was up-regulated, but the serum endotoxin levels and expression of pro-inflammatory factor (TNF-a, IL-6, and IL-1β) were down-regulated.
32433917	10	20	theme	seawater	1521:1528	arg1	immersion					1530:1538	seawater immersion	1521:1538	seawater immersion	1521:1538	In summary, we believe that CS/GEL/GMs-CIP composite scaffold may serve as a promising multifunctional dressing for healing with open trauma wound infections and wound with seawater immersion.
32433917	5	21	theme	water	580:584	arg1	ability					597:603	high water absorption ability	575:603	high water absorption ability	575:603	From in vitro characterization results, CS/GEL/GMs-CIP had high water absorption ability, proper porosity, satisfactory fracture resistance, and flexibility.
32433917	8	22	theme	animal	932:937	arg1	model					955:959	animal wound infection model	932:959	animal wound infection model of seawater immersion	932:981	The in vivo wound healing was evaluated using animal wound infection model of seawater immersion, and it was observed that the prepared composite scaffolds accelerated wound healing, reepithelialization, collagen deposition.
32433917	10	23	theme	open	1477:1480	arg1	infections					1495:1504	open trauma wound infections	1477:1504	open trauma wound infections	1477:1504	In summary, we believe that CS/GEL/GMs-CIP composite scaffold may serve as a promising multifunctional dressing for healing with open trauma wound infections and wound with seawater immersion.
32433917	5	24	theme	absorption	586:595	arg1	ability					597:603	high water absorption ability	575:603	high water absorption ability	575:603	From in vitro characterization results, CS/GEL/GMs-CIP had high water absorption ability, proper porosity, satisfactory fracture resistance, and flexibility.
32433917	6	25	theme	excellent	725:733	arg1	biocompatibility					735:750	excellent biocompatibility	725:750	excellent biocompatibility	725:750	Furthermore, CS/GEL/GMs-CIP composite scaffold had excellent biocompatibility.
32433917	9	26	theme	Further	1111:1117	arg1	analysis					1119:1126	Further analysis	1111:1126	Further analysis of wound tissue	1111:1142	Further analysis of wound tissue indicated that the expression of anti-inflammatory factor (TGF-β1) was up-regulated, but the serum endotoxin levels and expression of pro-inflammatory factor (TNF-a, IL-6, and IL-1β) were down-regulated.
32433917	0	27	theme	Antibiotic-loaded	0:16	arg1	chitosan-gelatin					18:33	Antibiotic-loaded chitosan-gelatin	0:33	Antibiotic-loaded chitosan-gelatin	0:33	Antibiotic-loaded chitosan-gelatin scaffolds for infected seawater immersion wound healing.
32433917	6	28	contain	had	721:723	arg1	scaffold					712:719	CS/GEL/GMs-CIP composite scaffold	687:719	CS/GEL/GMs-CIP composite scaffold	687:719	Furthermore, CS/GEL/GMs-CIP composite scaffold had excellent biocompatibility.
32433917	6	28	contain	had	721:723	arg2	biocompatibility					735:750	excellent biocompatibility	725:750	excellent biocompatibility	725:750	Furthermore, CS/GEL/GMs-CIP composite scaffold had excellent biocompatibility.
32433917	1	29	theme	sea	112:114	arg1	battles					116:122	sea battles	112:122	sea battles	112:122	Skin damaged during sea battles is vulnerable to seawater immersion and bacterial infection.
32433917	8	30	theme	infection	945:953	arg1	model					955:959	animal wound infection model	932:959	animal wound infection model of seawater immersion	932:981	The in vivo wound healing was evaluated using animal wound infection model of seawater immersion, and it was observed that the prepared composite scaffolds accelerated wound healing, reepithelialization, collagen deposition.
32433917	4	31	theme	gelatin	439:445	arg1	microspheres					447:458	gelatin microspheres	439:458	gelatin microspheres loading ciprofloxacin hydrochloride (GMs-CIP)	439:504	The chitosan (CS) and gelatin (GEL) were cross-linked by genipin as matrix, and then gelatin microspheres loading ciprofloxacin hydrochloride (GMs-CIP) were add.
32433917	8	32	theme	composite	1022:1030	arg1	scaffolds					1032:1040	the prepared composite scaffolds	1009:1040	the prepared composite scaffolds	1009:1040	The in vivo wound healing was evaluated using animal wound infection model of seawater immersion, and it was observed that the prepared composite scaffolds accelerated wound healing, reepithelialization, collagen deposition.
32433917	8	33	theme	wound	939:943	arg1	model					955:959	animal wound infection model	932:959	animal wound infection model of seawater immersion	932:981	The in vivo wound healing was evaluated using animal wound infection model of seawater immersion, and it was observed that the prepared composite scaffolds accelerated wound healing, reepithelialization, collagen deposition.
32433917	2	34	theme	biological	210:219	arg1	function					221:228	effective biological function	200:228	effective biological function	200:228	Scaffolds with effective biological function are highly desired for treatment of naval combat wound injuries.
32433917	9	35	theme	anti-inflammatory	1177:1193	arg1	TGF-β1					1203:1208	TGF-β1	1203:1208	TGF-β1	1203:1208	Further analysis of wound tissue indicated that the expression of anti-inflammatory factor (TGF-β1) was up-regulated, but the serum endotoxin levels and expression of pro-inflammatory factor (TNF-a, IL-6, and IL-1β) were down-regulated.
32433917	9	35	theme	anti-inflammatory	1177:1193	arg1	factor					1195:1200	anti-inflammatory factor	1177:1200	anti-inflammatory factor (TGF-β1)	1177:1209	Further analysis of wound tissue indicated that the expression of anti-inflammatory factor (TGF-β1) was up-regulated, but the serum endotoxin levels and expression of pro-inflammatory factor (TNF-a, IL-6, and IL-1β) were down-regulated.
32433917	5	36	dep	in	521:522	arg1	vitro					524:528	vitro	524:528	vitro	524:528	From in vitro characterization results, CS/GEL/GMs-CIP had high water absorption ability, proper porosity, satisfactory fracture resistance, and flexibility.
32433917	0	37	theme	seawater	58:65	arg1	healing					83:89	infected seawater immersion wound healing	49:89	infected seawater immersion wound healing	49:89	Antibiotic-loaded chitosan-gelatin scaffolds for infected seawater immersion wound healing.
32433917	5	38	contain	had	571:573	arg1	CS/GEL/GMs-CIP					556:569	CS/GEL/GMs-CIP	556:569	CS/GEL/GMs-CIP	556:569	From in vitro characterization results, CS/GEL/GMs-CIP had high water absorption ability, proper porosity, satisfactory fracture resistance, and flexibility.
32433917	5	38	contain	had	571:573	arg2	porosity					613:620	proper porosity	606:620	proper porosity	606:620	From in vitro characterization results, CS/GEL/GMs-CIP had high water absorption ability, proper porosity, satisfactory fracture resistance, and flexibility.
32433917	5	38	contain	had	571:573	arg2	flexibility					661:671	flexibility	661:671	flexibility	661:671	From in vitro characterization results, CS/GEL/GMs-CIP had high water absorption ability, proper porosity, satisfactory fracture resistance, and flexibility.
32433917	5	38	contain	had	571:573	arg2	resistance					645:654	satisfactory fracture resistance	623:654	satisfactory fracture resistance	623:654	From in vitro characterization results, CS/GEL/GMs-CIP had high water absorption ability, proper porosity, satisfactory fracture resistance, and flexibility.
32433917	5	38	contain	had	571:573	arg2	ability					597:603	high water absorption ability	575:603	high water absorption ability	575:603	From in vitro characterization results, CS/GEL/GMs-CIP had high water absorption ability, proper porosity, satisfactory fracture resistance, and flexibility.
32433917	2	39	theme	effective	200:208	arg1	function					221:228	effective biological function	200:228	effective biological function	200:228	Scaffolds with effective biological function are highly desired for treatment of naval combat wound injuries.
32433917	2	40	theme	naval	266:270	arg1	injuries					285:292	naval combat wound injuries	266:292	naval combat wound injuries	266:292	Scaffolds with effective biological function are highly desired for treatment of naval combat wound injuries.
32433917	6	41	theme	CS/GEL/GMs-CIP	687:700	arg1	scaffold					712:719	CS/GEL/GMs-CIP composite scaffold	687:719	CS/GEL/GMs-CIP composite scaffold	687:719	Furthermore, CS/GEL/GMs-CIP composite scaffold had excellent biocompatibility.
32433917	0	42	theme	infected	49:56	arg1	healing					83:89	infected seawater immersion wound healing	49:89	infected seawater immersion wound healing	49:89	Antibiotic-loaded chitosan-gelatin scaffolds for infected seawater immersion wound healing.
32433917	4	43	theme	ciprofloxacin	468:480	arg1	GMs-CIP					497:503	GMs-CIP	497:503	GMs-CIP	497:503	The chitosan (CS) and gelatin (GEL) were cross-linked by genipin as matrix, and then gelatin microspheres loading ciprofloxacin hydrochloride (GMs-CIP) were add.
32433917	4	43	theme	ciprofloxacin	468:480	arg1	hydrochloride					482:494	loading ciprofloxacin hydrochloride	460:494	gelatin microspheres loading ciprofloxacin hydrochloride (GMs-CIP)	439:504	The chitosan (CS) and gelatin (GEL) were cross-linked by genipin as matrix, and then gelatin microspheres loading ciprofloxacin hydrochloride (GMs-CIP) were add.
32433917	8	44	theme	immersion	973:981	arg1	model					955:959	animal wound infection model	932:959	animal wound infection model of seawater immersion	932:981	The in vivo wound healing was evaluated using animal wound infection model of seawater immersion, and it was observed that the prepared composite scaffolds accelerated wound healing, reepithelialization, collagen deposition.
32433917	0	45	theme	wound	77:81	arg1	healing					83:89	infected seawater immersion wound healing	49:89	infected seawater immersion wound healing	49:89	Antibiotic-loaded chitosan-gelatin scaffolds for infected seawater immersion wound healing.
32433917	4	46	theme	loading	460:466	arg1	GMs-CIP					497:503	GMs-CIP	497:503	GMs-CIP	497:503	The chitosan (CS) and gelatin (GEL) were cross-linked by genipin as matrix, and then gelatin microspheres loading ciprofloxacin hydrochloride (GMs-CIP) were add.
32433917	4	46	theme	loading	460:466	arg1	hydrochloride					482:494	loading ciprofloxacin hydrochloride	460:494	gelatin microspheres loading ciprofloxacin hydrochloride (GMs-CIP)	439:504	The chitosan (CS) and gelatin (GEL) were cross-linked by genipin as matrix, and then gelatin microspheres loading ciprofloxacin hydrochloride (GMs-CIP) were add.
32433917	10	47	theme	CS/GEL/GMs-CIP	1376:1389	arg1	dressing					1451:1458	a promising multifunctional dressing	1423:1458	a promising multifunctional dressing for healing with open trauma wound infections and wound with seawater immersion	1423:1538	In summary, we believe that CS/GEL/GMs-CIP composite scaffold may serve as a promising multifunctional dressing for healing with open trauma wound infections and wound with seawater immersion.
32433917	10	47	theme	CS/GEL/GMs-CIP	1376:1389	arg1	scaffold					1401:1408	CS/GEL/GMs-CIP composite scaffold	1376:1408	CS/GEL/GMs-CIP composite scaffold	1376:1408	In summary, we believe that CS/GEL/GMs-CIP composite scaffold may serve as a promising multifunctional dressing for healing with open trauma wound infections and wound with seawater immersion.
32433917	8	48	theme	seawater	964:971	arg1	immersion					973:981	seawater immersion	964:981	seawater immersion	964:981	The in vivo wound healing was evaluated using animal wound infection model of seawater immersion, and it was observed that the prepared composite scaffolds accelerated wound healing, reepithelialization, collagen deposition.
32433917	0	49	theme	immersion	67:75	arg1	healing					83:89	infected seawater immersion wound healing	49:89	infected seawater immersion wound healing	49:89	Antibiotic-loaded chitosan-gelatin scaffolds for infected seawater immersion wound healing.
32433917	10	50	theme	multifunctional	1435:1449	arg1	dressing					1451:1458	a promising multifunctional dressing	1423:1458	a promising multifunctional dressing for healing with open trauma wound infections and wound with seawater immersion	1423:1538	In summary, we believe that CS/GEL/GMs-CIP composite scaffold may serve as a promising multifunctional dressing for healing with open trauma wound infections and wound with seawater immersion.
32433917	10	50	theme	multifunctional	1435:1449	arg1	scaffold					1401:1408	CS/GEL/GMs-CIP composite scaffold	1376:1408	CS/GEL/GMs-CIP composite scaffold	1376:1408	In summary, we believe that CS/GEL/GMs-CIP composite scaffold may serve as a promising multifunctional dressing for healing with open trauma wound infections and wound with seawater immersion.
32433917	3	51	theme	CS/GEL/GMs-CIP	338:351	arg1	scaffolds					325:333	composite scaffolds	315:333	composite scaffolds of CS/GEL/GMs-CIP	315:351	Herein, we prepared composite scaffolds of CS/GEL/GMs-CIP.
32433917	2	52	with	Scaffolds	185:193	arg1	function					221:228	effective biological function	200:228	effective biological function	200:228	Scaffolds with effective biological function are highly desired for treatment of naval combat wound injuries.
32433917	9	53	theme	pro-inflammatory	1278:1293	arg1	factor					1295:1300	pro-inflammatory factor	1278:1300	pro-inflammatory factor (TNF-a, IL-6, and IL-1β)	1278:1325	Further analysis of wound tissue indicated that the expression of anti-inflammatory factor (TGF-β1) was up-regulated, but the serum endotoxin levels and expression of pro-inflammatory factor (TNF-a, IL-6, and IL-1β) were down-regulated.
32433917	3	54	theme	composite	315:323	arg1	scaffolds					325:333	composite scaffolds	315:333	composite scaffolds of CS/GEL/GMs-CIP	315:351	Herein, we prepared composite scaffolds of CS/GEL/GMs-CIP.
32433917	8	55	theme	wound	1054:1058	arg1	reepithelialization					1069:1087	reepithelialization	1069:1087	reepithelialization	1069:1087	The in vivo wound healing was evaluated using animal wound infection model of seawater immersion, and it was observed that the prepared composite scaffolds accelerated wound healing, reepithelialization, collagen deposition.
32433917	8	55	theme	wound	1054:1058	arg1	deposition					1099:1108	collagen deposition	1090:1108	collagen deposition	1090:1108	The in vivo wound healing was evaluated using animal wound infection model of seawater immersion, and it was observed that the prepared composite scaffolds accelerated wound healing, reepithelialization, collagen deposition.
32433917	8	55	theme	wound	1054:1058	arg1	healing					1060:1066	wound healing	1054:1066	wound healing	1054:1066	The in vivo wound healing was evaluated using animal wound infection model of seawater immersion, and it was observed that the prepared composite scaffolds accelerated wound healing, reepithelialization, collagen deposition.
32433917	5	56	theme	high	575:578	arg1	ability					597:603	high water absorption ability	575:603	high water absorption ability	575:603	From in vitro characterization results, CS/GEL/GMs-CIP had high water absorption ability, proper porosity, satisfactory fracture resistance, and flexibility.
32433917	1	57	theme	seawater	141:148	arg1	immersion					150:158	seawater immersion	141:158	seawater immersion	141:158	Skin damaged during sea battles is vulnerable to seawater immersion and bacterial infection.
32433917	7	58	theme	significant	815:825	arg1	effect					838:843	a significant inhibitory effect	813:843	a significant inhibitory effect	813:843	Antibacterial experiments confirmed that CS/GEL/GMs-CIP had a significant inhibitory effect on E. coli, S. aureus and P. aeruginosa.
32433917	8	59	dep	in	890:891	arg1	vivo					893:896	vivo	893:896	vivo	893:896	The in vivo wound healing was evaluated using animal wound infection model of seawater immersion, and it was observed that the prepared composite scaffolds accelerated wound healing, reepithelialization, collagen deposition.
32433917	2	60	theme	injuries	285:292	arg1	treatment					253:261	treatment	253:261	treatment of naval combat wound injuries	253:292	Scaffolds with effective biological function are highly desired for treatment of naval combat wound injuries.
32433917	5	61	theme	in	521:522	arg1	results					547:553	in vitro characterization results	521:553	in vitro characterization results	521:553	From in vitro characterization results, CS/GEL/GMs-CIP had high water absorption ability, proper porosity, satisfactory fracture resistance, and flexibility.
32433917	10	62	with	wound	1510:1514	arg1	immersion					1530:1538	seawater immersion	1521:1538	seawater immersion	1521:1538	In summary, we believe that CS/GEL/GMs-CIP composite scaffold may serve as a promising multifunctional dressing for healing with open trauma wound infections and wound with seawater immersion.
32433917	10	63	theme	composite	1391:1399	arg1	dressing					1451:1458	a promising multifunctional dressing	1423:1458	a promising multifunctional dressing for healing with open trauma wound infections and wound with seawater immersion	1423:1538	In summary, we believe that CS/GEL/GMs-CIP composite scaffold may serve as a promising multifunctional dressing for healing with open trauma wound infections and wound with seawater immersion.
32433917	10	63	theme	composite	1391:1399	arg1	scaffold					1401:1408	CS/GEL/GMs-CIP composite scaffold	1376:1408	CS/GEL/GMs-CIP composite scaffold	1376:1408	In summary, we believe that CS/GEL/GMs-CIP composite scaffold may serve as a promising multifunctional dressing for healing with open trauma wound infections and wound with seawater immersion.
32433917	7	64	theme	inhibitory	827:836	arg1	effect					838:843	a significant inhibitory effect	813:843	a significant inhibitory effect	813:843	Antibacterial experiments confirmed that CS/GEL/GMs-CIP had a significant inhibitory effect on E. coli, S. aureus and P. aeruginosa.
32433917	2	65	theme	wound	279:283	arg1	injuries					285:292	naval combat wound injuries	266:292	naval combat wound injuries	266:292	Scaffolds with effective biological function are highly desired for treatment of naval combat wound injuries.
32433917	10	66	theme	promising	1425:1433	arg1	dressing					1451:1458	a promising multifunctional dressing	1423:1458	a promising multifunctional dressing for healing with open trauma wound infections and wound with seawater immersion	1423:1538	In summary, we believe that CS/GEL/GMs-CIP composite scaffold may serve as a promising multifunctional dressing for healing with open trauma wound infections and wound with seawater immersion.
32433917	10	66	theme	promising	1425:1433	arg1	scaffold					1401:1408	CS/GEL/GMs-CIP composite scaffold	1376:1408	CS/GEL/GMs-CIP composite scaffold	1376:1408	In summary, we believe that CS/GEL/GMs-CIP composite scaffold may serve as a promising multifunctional dressing for healing with open trauma wound infections and wound with seawater immersion.
32433917	9	67	dep	factor	1295:1300	arg1	TNF-a					1303:1307	TNF-a	1303:1307	TNF-a	1303:1307	Further analysis of wound tissue indicated that the expression of anti-inflammatory factor (TGF-β1) was up-regulated, but the serum endotoxin levels and expression of pro-inflammatory factor (TNF-a, IL-6, and IL-1β) were down-regulated.
32433917	9	67	dep	factor	1295:1300	arg1	IL-6					1310:1313	IL-6	1310:1313	IL-6	1310:1313	Further analysis of wound tissue indicated that the expression of anti-inflammatory factor (TGF-β1) was up-regulated, but the serum endotoxin levels and expression of pro-inflammatory factor (TNF-a, IL-6, and IL-1β) were down-regulated.
32433917	9	67	dep	factor	1295:1300	arg1	IL-1β					1320:1324	IL-1β	1320:1324	IL-1β	1320:1324	Further analysis of wound tissue indicated that the expression of anti-inflammatory factor (TGF-β1) was up-regulated, but the serum endotoxin levels and expression of pro-inflammatory factor (TNF-a, IL-6, and IL-1β) were down-regulated.
32433917	2	68	theme	combat	272:277	arg1	injuries					285:292	naval combat wound injuries	266:292	naval combat wound injuries	266:292	Scaffolds with effective biological function are highly desired for treatment of naval combat wound injuries.
32433917	5	69	theme	characterization	530:545	arg1	results					547:553	in vitro characterization results	521:553	in vitro characterization results	521:553	From in vitro characterization results, CS/GEL/GMs-CIP had high water absorption ability, proper porosity, satisfactory fracture resistance, and flexibility.
32433917	1	70	theme	bacterial	164:172	arg1	infection					174:182	bacterial infection	164:182	bacterial infection	164:182	Skin damaged during sea battles is vulnerable to seawater immersion and bacterial infection.
32433917	7	71	theme	Antibacterial	753:765	arg1	experiments					767:777	Antibacterial experiments	753:777	Antibacterial experiments	753:777	Antibacterial experiments confirmed that CS/GEL/GMs-CIP had a significant inhibitory effect on E. coli, S. aureus and P. aeruginosa.
34099602	2	0	theme	liquid-state	328:339	arg1	fermentation					341:352	liquid-state fermentation	328:352	liquid-state fermentation	328:352	Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation.
34099602	3	1	from	time	480:483	arg1	factor					468:473	one factor	464:473	one factor at a time experiments	464:495	To determine the optimal concentrations of these components for enzyme and β-glucan production, we conducted one factor at a time experiments, which showed that the optimal concentrations were 30 g/l brown rice, 30 g/l rice bran, 10 g/l soytone, and 3 g/l ascorbic acid.
34099602	1	2	theme	β-glucan	155:162	arg1	production					164:173	β-glucan production	155:173	β-glucan production by Aspergillus oryzae KCCM 12698	155:206	The effect of medium composition on enzyme and β-glucan production by Aspergillus oryzae KCCM 12698 was investigated.
34099602	0	3	theme	Rice	102:105	arg1	Fermentation					80:91	Solid-State Fermentation	68:91	Solid-State Fermentation of Brown Rice	68:105	Coproduction of Enzymes and Beta-Glucan by Aspergillus oryzae Using Solid-State Fermentation of Brown Rice.
34099602	2	4	theme	Brown	226:230	arg1	rice					232:235	Brown rice	226:235	Brown rice	226:235	Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation.
34099602	2	4	theme	Brown	226:230	arg1	bran					243:246	rice bran	238:246	rice bran	238:246	Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation.
34099602	2	4	theme	Brown	226:230	arg1	acid					272:275	ascorbic acid	263:275	ascorbic acid	263:275	Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation.
34099602	2	4	theme	Brown	226:230	arg1	components					285:294	key components	281:294	key components of the synthetic medium used in liquid-state fermentation	281:352	Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation.
34099602	2	4	theme	Brown	226:230	arg1	nitrogen					249:256	nitrogen	249:256	nitrogen	249:256	Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation.
34099602	4	5	theme	fungal	694:699	arg1	biomass					701:707	fungal biomass	694:707	fungal biomass	694:707	Pretreatment of brown rice for 60 min prior to inoculation enhanced fungal biomass, while increasing the production of enzymes and β-glucan using solidstate fermentation.
34099602	6	6	from	use	1085:1087	arg1	applications					1100:1111	various applications	1092:1111	various applications	1092:1111	Therefore, solidstate fermentation of brown rice is a process that could enhance yield and overall production of enzymes and β-glucan for use in various applications.
34099602	3	7	dep	g/l	607:609	arg1	acid					620:623	ascorbic acid	611:623	3 g/l ascorbic acid	605:623	To determine the optimal concentrations of these components for enzyme and β-glucan production, we conducted one factor at a time experiments, which showed that the optimal concentrations were 30 g/l brown rice, 30 g/l rice bran, 10 g/l soytone, and 3 g/l ascorbic acid.
34099602	0	8	theme	Brown	96:100	arg1	Rice					102:105	Brown Rice	96:105	Brown Rice	96:105	Coproduction of Enzymes and Beta-Glucan by Aspergillus oryzae Using Solid-State Fermentation of Brown Rice.
34099602	3	9	theme	optimal	520:526	arg1	rice					561:564	30 g/l brown rice	548:564	30 g/l brown rice	548:564	To determine the optimal concentrations of these components for enzyme and β-glucan production, we conducted one factor at a time experiments, which showed that the optimal concentrations were 30 g/l brown rice, 30 g/l rice bran, 10 g/l soytone, and 3 g/l ascorbic acid.
34099602	3	9	theme	optimal	520:526	arg1	concentrations					528:541	the optimal concentrations	516:541	the optimal concentrations	516:541	To determine the optimal concentrations of these components for enzyme and β-glucan production, we conducted one factor at a time experiments, which showed that the optimal concentrations were 30 g/l brown rice, 30 g/l rice bran, 10 g/l soytone, and 3 g/l ascorbic acid.
34099602	2	10	theme	ascorbic	263:270	arg1	rice					232:235	Brown rice	226:235	Brown rice	226:235	Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation.
34099602	2	10	theme	ascorbic	263:270	arg1	bran					243:246	rice bran	238:246	rice bran	238:246	Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation.
34099602	2	10	theme	ascorbic	263:270	arg1	acid					272:275	ascorbic acid	263:275	ascorbic acid	263:275	Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation.
34099602	2	10	theme	ascorbic	263:270	arg1	components					285:294	key components	281:294	key components of the synthetic medium used in liquid-state fermentation	281:352	Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation.
34099602	2	10	theme	ascorbic	263:270	arg1	nitrogen					249:256	nitrogen	249:256	nitrogen	249:256	Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation.
34099602	3	11	theme	factor	468:473	arg1	experiments					485:495	one factor at a time experiments	464:495	one factor at a time experiments	464:495	To determine the optimal concentrations of these components for enzyme and β-glucan production, we conducted one factor at a time experiments, which showed that the optimal concentrations were 30 g/l brown rice, 30 g/l rice bran, 10 g/l soytone, and 3 g/l ascorbic acid.
34099602	4	12	theme	solidstate	772:781	arg1	fermentation					783:794	solidstate fermentation	772:794	solidstate fermentation	772:794	Pretreatment of brown rice for 60 min prior to inoculation enhanced fungal biomass, while increasing the production of enzymes and β-glucan using solidstate fermentation.
34099602	6	13	theme	brown	985:989	arg1	rice					991:994	brown rice	985:994	brown rice	985:994	Therefore, solidstate fermentation of brown rice is a process that could enhance yield and overall production of enzymes and β-glucan for use in various applications.
34099602	5	14	from	%	904:904	arg1	U/g					853:855	26,551.03 U/g	843:855	26,551.03 U/g	843:855	Maximum fungal biomass of 0.76 mg/g, amylase (26,551.03 U/g), protease (1,340.50 U/g), and β-glucan at 9.34% (w/w) were obtained during fermentation.
34099602	5	14	from	%	904:904	arg1	U/g					878:880	1,340.50 U/g	869:880	1,340.50 U/g	869:880	Maximum fungal biomass of 0.76 mg/g, amylase (26,551.03 U/g), protease (1,340.50 U/g), and β-glucan at 9.34% (w/w) were obtained during fermentation.
34099602	5	14	from	%	904:904	arg1	amylase					834:840	amylase	834:840	amylase (26,551.03 U/g)	834:856	Maximum fungal biomass of 0.76 mg/g, amylase (26,551.03 U/g), protease (1,340.50 U/g), and β-glucan at 9.34% (w/w) were obtained during fermentation.
34099602	5	14	from	%	904:904	arg1	protease					859:866	protease	859:866	protease (1,340.50 U/g)	859:881	Maximum fungal biomass of 0.76 mg/g, amylase (26,551.03 U/g), protease (1,340.50 U/g), and β-glucan at 9.34% (w/w) were obtained during fermentation.
34099602	5	14	from	%	904:904	arg1	β-glucan					888:895	β-glucan	888:895	β-glucan at 9.34% (w/w)	888:910	Maximum fungal biomass of 0.76 mg/g, amylase (26,551.03 U/g), protease (1,340.50 U/g), and β-glucan at 9.34% (w/w) were obtained during fermentation.
34099602	5	14	from	%	904:904	arg1	biomass					812:818	Maximum fungal biomass	797:818	Maximum fungal biomass of 0.76 mg/g	797:831	Maximum fungal biomass of 0.76 mg/g, amylase (26,551.03 U/g), protease (1,340.50 U/g), and β-glucan at 9.34% (w/w) were obtained during fermentation.
34099602	5	15	theme	Maximum	797:803	arg1	biomass					812:818	Maximum fungal biomass	797:818	Maximum fungal biomass of 0.76 mg/g	797:831	Maximum fungal biomass of 0.76 mg/g, amylase (26,551.03 U/g), protease (1,340.50 U/g), and β-glucan at 9.34% (w/w) were obtained during fermentation.
34099602	3	16	theme	β-glucan	430:437	arg1	production					439:448	β-glucan production	430:448	β-glucan production	430:448	To determine the optimal concentrations of these components for enzyme and β-glucan production, we conducted one factor at a time experiments, which showed that the optimal concentrations were 30 g/l brown rice, 30 g/l rice bran, 10 g/l soytone, and 3 g/l ascorbic acid.
34099602	6	17	theme	overall	1038:1044	arg1	production					1046:1055	overall production	1038:1055	overall production	1038:1055	Therefore, solidstate fermentation of brown rice is a process that could enhance yield and overall production of enzymes and β-glucan for use in various applications.
34099602	3	18	theme	30	548:549	arg1	g/l					551:553	g/l	551:553	g/l	551:553	To determine the optimal concentrations of these components for enzyme and β-glucan production, we conducted one factor at a time experiments, which showed that the optimal concentrations were 30 g/l brown rice, 30 g/l rice bran, 10 g/l soytone, and 3 g/l ascorbic acid.
34099602	1	19	theme	Aspergillus	178:188	arg1	oryzae					190:195	Aspergillus oryzae	178:195	Aspergillus oryzae KCCM 12698	178:206	The effect of medium composition on enzyme and β-glucan production by Aspergillus oryzae KCCM 12698 was investigated.
34099602	3	20	theme	optimal	372:378	arg1	concentrations					380:393	the optimal concentrations	368:393	the optimal concentrations of these components for enzyme and β-glucan production	368:448	To determine the optimal concentrations of these components for enzyme and β-glucan production, we conducted one factor at a time experiments, which showed that the optimal concentrations were 30 g/l brown rice, 30 g/l rice bran, 10 g/l soytone, and 3 g/l ascorbic acid.
34099602	1	21	from	effect	112:117	arg1	enzyme					144:149	enzyme	144:149	enzyme	144:149	The effect of medium composition on enzyme and β-glucan production by Aspergillus oryzae KCCM 12698 was investigated.
34099602	1	21	from	effect	112:117	arg1	production					164:173	β-glucan production	155:173	β-glucan production by Aspergillus oryzae KCCM 12698	155:206	The effect of medium composition on enzyme and β-glucan production by Aspergillus oryzae KCCM 12698 was investigated.
34099602	3	22	theme	g/l	551:553	arg1	rice					561:564	30 g/l brown rice	548:564	30 g/l brown rice	548:564	To determine the optimal concentrations of these components for enzyme and β-glucan production, we conducted one factor at a time experiments, which showed that the optimal concentrations were 30 g/l brown rice, 30 g/l rice bran, 10 g/l soytone, and 3 g/l ascorbic acid.
34099602	3	22	theme	g/l	551:553	arg1	concentrations					528:541	the optimal concentrations	516:541	the optimal concentrations	516:541	To determine the optimal concentrations of these components for enzyme and β-glucan production, we conducted one factor at a time experiments, which showed that the optimal concentrations were 30 g/l brown rice, 30 g/l rice bran, 10 g/l soytone, and 3 g/l ascorbic acid.
34099602	1	23	theme	oryzae	190:195	arg1	12698					202:206	Aspergillus oryzae KCCM 12698	178:206	Aspergillus oryzae KCCM 12698	178:206	The effect of medium composition on enzyme and β-glucan production by Aspergillus oryzae KCCM 12698 was investigated.
34099602	6	24	theme	solidstate	958:967	arg1	process					1001:1007	a process	999:1007	a process that could enhance yield and overall production of enzymes and β-glucan for use in various applications	999:1111	Therefore, solidstate fermentation of brown rice is a process that could enhance yield and overall production of enzymes and β-glucan for use in various applications.
34099602	6	24	theme	solidstate	958:967	arg1	fermentation					969:980	solidstate fermentation	958:980	solidstate fermentation of brown rice	958:994	Therefore, solidstate fermentation of brown rice is a process that could enhance yield and overall production of enzymes and β-glucan for use in various applications.
34099602	2	25	theme	key	281:283	arg1	rice					232:235	Brown rice	226:235	Brown rice	226:235	Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation.
34099602	2	25	theme	key	281:283	arg1	bran					243:246	rice bran	238:246	rice bran	238:246	Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation.
34099602	2	25	theme	key	281:283	arg1	acid					272:275	ascorbic acid	263:275	ascorbic acid	263:275	Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation.
34099602	2	25	theme	key	281:283	arg1	components					285:294	key components	281:294	key components of the synthetic medium used in liquid-state fermentation	281:352	Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation.
34099602	2	25	theme	key	281:283	arg1	nitrogen					249:256	nitrogen	249:256	nitrogen	249:256	Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation.
34099602	1	26	theme	KCCM	197:200	arg1	12698					202:206	Aspergillus oryzae KCCM 12698	178:206	Aspergillus oryzae KCCM 12698	178:206	The effect of medium composition on enzyme and β-glucan production by Aspergillus oryzae KCCM 12698 was investigated.
34099602	0	27	theme	Enzymes	16:22	arg1	Coproduction					0:11	Coproduction	0:11	Coproduction of Enzymes and Beta-Glucan by Aspergillus oryzae Using Solid-State Fermentation of Brown Rice.	0:106	Coproduction of Enzymes and Beta-Glucan by Aspergillus oryzae Using Solid-State Fermentation of Brown Rice.
34099602	4	28	theme	brown	642:646	arg1	rice					648:651	brown rice	642:651	brown rice	642:651	Pretreatment of brown rice for 60 min prior to inoculation enhanced fungal biomass, while increasing the production of enzymes and β-glucan using solidstate fermentation.
34099602	4	29	theme	enzymes	745:751	arg1	production					731:740	the production	727:740	the production of enzymes	727:751	Pretreatment of brown rice for 60 min prior to inoculation enhanced fungal biomass, while increasing the production of enzymes and β-glucan using solidstate fermentation.
34099602	4	29	theme	enzymes	745:751	arg1	β-glucan					757:764	β-glucan	757:764	β-glucan	757:764	Pretreatment of brown rice for 60 min prior to inoculation enhanced fungal biomass, while increasing the production of enzymes and β-glucan using solidstate fermentation.
34099602	0	30	theme	Beta-Glucan	28:38	arg1	Coproduction					0:11	Coproduction	0:11	Coproduction of Enzymes and Beta-Glucan by Aspergillus oryzae Using Solid-State Fermentation of Brown Rice.	0:106	Coproduction of Enzymes and Beta-Glucan by Aspergillus oryzae Using Solid-State Fermentation of Brown Rice.
34099602	3	31	theme	components	404:413	arg1	concentrations					380:393	the optimal concentrations	368:393	the optimal concentrations of these components for enzyme and β-glucan production	368:448	To determine the optimal concentrations of these components for enzyme and β-glucan production, we conducted one factor at a time experiments, which showed that the optimal concentrations were 30 g/l brown rice, 30 g/l rice bran, 10 g/l soytone, and 3 g/l ascorbic acid.
34099602	3	32	theme	10	585:586	arg1	g/l					588:590	g/l	588:590	g/l	588:590	To determine the optimal concentrations of these components for enzyme and β-glucan production, we conducted one factor at a time experiments, which showed that the optimal concentrations were 30 g/l brown rice, 30 g/l rice bran, 10 g/l soytone, and 3 g/l ascorbic acid.
34099602	3	33	theme	30	567:568	arg1	g/l					570:572	g/l	570:572	g/l	570:572	To determine the optimal concentrations of these components for enzyme and β-glucan production, we conducted one factor at a time experiments, which showed that the optimal concentrations were 30 g/l brown rice, 30 g/l rice bran, 10 g/l soytone, and 3 g/l ascorbic acid.
34099602	3	34	theme	g/l	588:590	arg1	soytone					592:598	10 g/l soytone	585:598	10 g/l soytone	585:598	To determine the optimal concentrations of these components for enzyme and β-glucan production, we conducted one factor at a time experiments, which showed that the optimal concentrations were 30 g/l brown rice, 30 g/l rice bran, 10 g/l soytone, and 3 g/l ascorbic acid.
34099602	3	35	theme	brown	555:559	arg1	rice					561:564	30 g/l brown rice	548:564	30 g/l brown rice	548:564	To determine the optimal concentrations of these components for enzyme and β-glucan production, we conducted one factor at a time experiments, which showed that the optimal concentrations were 30 g/l brown rice, 30 g/l rice bran, 10 g/l soytone, and 3 g/l ascorbic acid.
34099602	3	35	theme	brown	555:559	arg1	concentrations					528:541	the optimal concentrations	516:541	the optimal concentrations	516:541	To determine the optimal concentrations of these components for enzyme and β-glucan production, we conducted one factor at a time experiments, which showed that the optimal concentrations were 30 g/l brown rice, 30 g/l rice bran, 10 g/l soytone, and 3 g/l ascorbic acid.
34099602	4	36	theme	rice	648:651	arg1	Pretreatment					626:637	Pretreatment	626:637	Pretreatment of brown rice for 60 min prior to inoculation	626:683	Pretreatment of brown rice for 60 min prior to inoculation enhanced fungal biomass, while increasing the production of enzymes and β-glucan using solidstate fermentation.
34099602	0	37	theme	Aspergillus	43:53	arg1	oryzae					55:60	Aspergillus oryzae	43:60	Aspergillus oryzae Using Solid-State Fermentation of Brown Rice	43:105	Coproduction of Enzymes and Beta-Glucan by Aspergillus oryzae Using Solid-State Fermentation of Brown Rice.
34099602	6	38	theme	enzymes	1060:1066	arg1	β-glucan					1072:1079	β-glucan	1072:1079	β-glucan for use in various applications	1072:1111	Therefore, solidstate fermentation of brown rice is a process that could enhance yield and overall production of enzymes and β-glucan for use in various applications.
34099602	6	38	theme	enzymes	1060:1066	arg1	yield					1028:1032	yield	1028:1032	yield	1028:1032	Therefore, solidstate fermentation of brown rice is a process that could enhance yield and overall production of enzymes and β-glucan for use in various applications.
34099602	6	38	theme	enzymes	1060:1066	arg1	production					1046:1055	overall production	1038:1055	overall production	1038:1055	Therefore, solidstate fermentation of brown rice is a process that could enhance yield and overall production of enzymes and β-glucan for use in various applications.
34099602	6	39	theme	various	1092:1098	arg1	applications					1100:1111	various applications	1092:1111	various applications	1092:1111	Therefore, solidstate fermentation of brown rice is a process that could enhance yield and overall production of enzymes and β-glucan for use in various applications.
34099602	2	40	theme	medium	313:318	arg1	rice					232:235	Brown rice	226:235	Brown rice	226:235	Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation.
34099602	2	40	theme	medium	313:318	arg1	bran					243:246	rice bran	238:246	rice bran	238:246	Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation.
34099602	2	40	theme	medium	313:318	arg1	acid					272:275	ascorbic acid	263:275	ascorbic acid	263:275	Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation.
34099602	2	40	theme	medium	313:318	arg1	components					285:294	key components	281:294	key components of the synthetic medium used in liquid-state fermentation	281:352	Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation.
34099602	2	40	theme	medium	313:318	arg1	nitrogen					249:256	nitrogen	249:256	nitrogen	249:256	Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation.
34099602	3	41	theme	rice	574:577	arg1	bran					579:582	30 g/l rice bran	567:582	30 g/l rice bran	567:582	To determine the optimal concentrations of these components for enzyme and β-glucan production, we conducted one factor at a time experiments, which showed that the optimal concentrations were 30 g/l brown rice, 30 g/l rice bran, 10 g/l soytone, and 3 g/l ascorbic acid.
34099602	3	42	theme	ascorbic	611:618	arg1	acid					620:623	ascorbic acid	611:623	3 g/l ascorbic acid	605:623	To determine the optimal concentrations of these components for enzyme and β-glucan production, we conducted one factor at a time experiments, which showed that the optimal concentrations were 30 g/l brown rice, 30 g/l rice bran, 10 g/l soytone, and 3 g/l ascorbic acid.
34099602	3	43	dep	conducted	454:462	arg1	showed					504:509	showed	504:509	showed that the optimal concentrations were 30 g/l brown rice, 30 g/l rice bran, 10 g/l soytone, and 3 g/l ascorbic acid	504:623	To determine the optimal concentrations of these components for enzyme and β-glucan production, we conducted one factor at a time experiments, which showed that the optimal concentrations were 30 g/l brown rice, 30 g/l rice bran, 10 g/l soytone, and 3 g/l ascorbic acid.
34099602	1	44	theme	medium	122:127	arg1	composition					129:139	medium composition	122:139	medium composition	122:139	The effect of medium composition on enzyme and β-glucan production by Aspergillus oryzae KCCM 12698 was investigated.
34099602	1	45	theme	composition	129:139	arg1	effect					112:117	The effect	108:117	The effect of medium composition on enzyme and β-glucan production by Aspergillus oryzae KCCM 12698	108:206	The effect of medium composition on enzyme and β-glucan production by Aspergillus oryzae KCCM 12698 was investigated.
34099602	2	46	theme	synthetic	303:311	arg1	medium					313:318	the synthetic medium	299:318	the synthetic medium used in liquid-state fermentation	299:352	Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation.
34099602	0	47	theme	Solid-State	68:78	arg1	Fermentation					80:91	Solid-State Fermentation	68:91	Solid-State Fermentation of Brown Rice	68:105	Coproduction of Enzymes and Beta-Glucan by Aspergillus oryzae Using Solid-State Fermentation of Brown Rice.
34099602	6	48	theme	rice	991:994	arg1	process					1001:1007	a process	999:1007	a process that could enhance yield and overall production of enzymes and β-glucan for use in various applications	999:1111	Therefore, solidstate fermentation of brown rice is a process that could enhance yield and overall production of enzymes and β-glucan for use in various applications.
34099602	6	48	theme	rice	991:994	arg1	fermentation					969:980	solidstate fermentation	958:980	solidstate fermentation of brown rice	958:994	Therefore, solidstate fermentation of brown rice is a process that could enhance yield and overall production of enzymes and β-glucan for use in various applications.
34099602	3	49	theme	g/l	570:572	arg1	bran					579:582	30 g/l rice bran	567:582	30 g/l rice bran	567:582	To determine the optimal concentrations of these components for enzyme and β-glucan production, we conducted one factor at a time experiments, which showed that the optimal concentrations were 30 g/l brown rice, 30 g/l rice bran, 10 g/l soytone, and 3 g/l ascorbic acid.
34099602	5	50	theme	fungal	805:810	arg1	biomass					812:818	Maximum fungal biomass	797:818	Maximum fungal biomass of 0.76 mg/g	797:831	Maximum fungal biomass of 0.76 mg/g, amylase (26,551.03 U/g), protease (1,340.50 U/g), and β-glucan at 9.34% (w/w) were obtained during fermentation.
34099602	2	51	theme	rice	238:241	arg1	rice					232:235	Brown rice	226:235	Brown rice	226:235	Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation.
34099602	2	51	theme	rice	238:241	arg1	bran					243:246	rice bran	238:246	rice bran	238:246	Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation.
34099602	2	51	theme	rice	238:241	arg1	acid					272:275	ascorbic acid	263:275	ascorbic acid	263:275	Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation.
34099602	2	51	theme	rice	238:241	arg1	components					285:294	key components	281:294	key components of the synthetic medium used in liquid-state fermentation	281:352	Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation.
34099602	2	51	theme	rice	238:241	arg1	nitrogen					249:256	nitrogen	249:256	nitrogen	249:256	Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation.
34099602	5	52	theme	mg/g	828:831	arg1	U/g					853:855	26,551.03 U/g	843:855	26,551.03 U/g	843:855	Maximum fungal biomass of 0.76 mg/g, amylase (26,551.03 U/g), protease (1,340.50 U/g), and β-glucan at 9.34% (w/w) were obtained during fermentation.
34099602	5	52	theme	mg/g	828:831	arg1	U/g					878:880	1,340.50 U/g	869:880	1,340.50 U/g	869:880	Maximum fungal biomass of 0.76 mg/g, amylase (26,551.03 U/g), protease (1,340.50 U/g), and β-glucan at 9.34% (w/w) were obtained during fermentation.
34099602	5	52	theme	mg/g	828:831	arg1	amylase					834:840	amylase	834:840	amylase (26,551.03 U/g)	834:856	Maximum fungal biomass of 0.76 mg/g, amylase (26,551.03 U/g), protease (1,340.50 U/g), and β-glucan at 9.34% (w/w) were obtained during fermentation.
34099602	5	52	theme	mg/g	828:831	arg1	protease					859:866	protease	859:866	protease (1,340.50 U/g)	859:881	Maximum fungal biomass of 0.76 mg/g, amylase (26,551.03 U/g), protease (1,340.50 U/g), and β-glucan at 9.34% (w/w) were obtained during fermentation.
34099602	5	52	theme	mg/g	828:831	arg1	β-glucan					888:895	β-glucan	888:895	β-glucan at 9.34% (w/w)	888:910	Maximum fungal biomass of 0.76 mg/g, amylase (26,551.03 U/g), protease (1,340.50 U/g), and β-glucan at 9.34% (w/w) were obtained during fermentation.
34099602	5	52	theme	mg/g	828:831	arg1	biomass					812:818	Maximum fungal biomass	797:818	Maximum fungal biomass of 0.76 mg/g	797:831	Maximum fungal biomass of 0.76 mg/g, amylase (26,551.03 U/g), protease (1,340.50 U/g), and β-glucan at 9.34% (w/w) were obtained during fermentation.
34064563	0	0	theme	Bacillus	87:94	arg1	sp					96:97	Halophilic Bacillus sp	76:97	Halophilic Bacillus sp	76:97	Statistical Based Bioprocess Design for Improved Production of Amylase from Halophilic Bacillus sp.
34064563	4	1	theme	various	496:502	arg1	components					511:520	various medium components	496:520	various medium components	496:520	The use of Plackett-Burman design (PBD) that evaluates various medium components and having two-level factorial designs help to determine the factor and its level to increase the yield of product.
34064563	0	2	theme	Halophilic	76:85	arg1	sp					96:97	Halophilic Bacillus sp	76:97	Halophilic Bacillus sp	76:97	Statistical Based Bioprocess Design for Improved Production of Amylase from Halophilic Bacillus sp.
34064563	4	3	theme	two-level	533:541	arg1	designs					553:559	two-level factorial designs	533:559	two-level factorial designs	533:559	The use of Plackett-Burman design (PBD) that evaluates various medium components and having two-level factorial designs help to determine the factor and its level to increase the yield of product.
34064563	8	4	from	increase	968:975	arg1	production					988:997	amylase production	980:997	amylase production	980:997	A 1.31-fold increase in amylase production was evident using a 5.0 L laboratory-scale bioreactor.
34064563	2	5	from	demand	181:186	arg1	industries					199:208	various industries	191:208	various industries	191:208	Amylase (EC 3.2.1.1) enzyme has gained tremendous demand in various industries, including wastewater treatment, bioremediation and nano-biotechnology.
34064563	2	5	from	demand	181:186	arg1	bioremediation					243:256	bioremediation	243:256	bioremediation	243:256	Amylase (EC 3.2.1.1) enzyme has gained tremendous demand in various industries, including wastewater treatment, bioremediation and nano-biotechnology.
34064563	2	5	from	demand	181:186	arg1	nano-biotechnology					262:279	nano-biotechnology	262:279	nano-biotechnology	262:279	Amylase (EC 3.2.1.1) enzyme has gained tremendous demand in various industries, including wastewater treatment, bioremediation and nano-biotechnology.
34064563	2	5	from	demand	181:186	arg1	treatment					232:240	wastewater treatment	221:240	wastewater treatment	221:240	Amylase (EC 3.2.1.1) enzyme has gained tremendous demand in various industries, including wastewater treatment, bioremediation and nano-biotechnology.
34064563	7	6	dep	PBD	831:833	arg1	i.e.					825:828	i.e.	825:828	i.e.	825:828	The use of two-stage statistical optimization, i.e., PBD and response surface methodology (RSM), using central composite design (CCD) further improved the production of amylase.
34064563	8	7	theme	laboratory-scale	1025:1040	arg1	bioreactor					1042:1051	a 5.0 L laboratory-scale bioreactor	1017:1051	a 5.0 L laboratory-scale bioreactor	1017:1051	A 1.31-fold increase in amylase production was evident using a 5.0 L laboratory-scale bioreactor.
34064563	8	8	theme	1.31-fold	958:966	arg1	evident					1003:1009	evident	1003:1009	evident	1003:1009	A 1.31-fold increase in amylase production was evident using a 5.0 L laboratory-scale bioreactor.
34064563	8	8	theme	1.31-fold	958:966	arg1	increase					968:975	A 1.31-fold increase	956:975	A 1.31-fold increase in amylase production	956:997	A 1.31-fold increase in amylase production was evident using a 5.0 L laboratory-scale bioreactor.
34064563	7	9	theme	surface	848:854	arg1	RSM					869:871	RSM	869:871	RSM	869:871	The use of two-stage statistical optimization, i.e., PBD and response surface methodology (RSM), using central composite design (CCD) further improved the production of amylase.
34064563	7	9	theme	surface	848:854	arg1	methodology					856:866	response surface methodology	839:866	response surface methodology (RSM)	839:872	The use of two-stage statistical optimization, i.e., PBD and response surface methodology (RSM), using central composite design (CCD) further improved the production of amylase.
34064563	3	10	from	compels	287:293	arg1	yields					333:338	greater yields	325:338	greater yields that can be achieved by employing potential amylase-producing cultures and statistical optimization	325:438	This compels the availability of enzyme in greater yields that can be achieved by employing potential amylase-producing cultures and statistical optimization.
34064563	5	11	theme	Bacillus	749:756	arg1	sp					758:759	Bacillus sp	749:759	Bacillus sp	749:759	In the present work, we are reporting the screening of amylase-producing marine bacterial strain identified as Bacillus sp.
34064563	8	12	theme	L	1023:1023	arg1	bioreactor					1042:1051	a 5.0 L laboratory-scale bioreactor	1017:1051	a 5.0 L laboratory-scale bioreactor	1017:1051	A 1.31-fold increase in amylase production was evident using a 5.0 L laboratory-scale bioreactor.
34064563	9	13	theme	Statistical	1054:1064	arg1	optimization					1066:1077	Statistical optimization	1054:1077	Statistical optimization	1054:1077	Statistical optimization gives the exact idea of variables that influence the production of enzymes, and hence, the statistical approach offers the best way to optimize the bioprocess.
34064563	4	14	theme	medium	504:509	arg1	components					511:520	various medium components	496:520	various medium components	496:520	The use of Plackett-Burman design (PBD) that evaluates various medium components and having two-level factorial designs help to determine the factor and its level to increase the yield of product.
34064563	11	15	theme	soluble	1314:1320	arg1	starch					1322:1327	soluble starch	1314:1327	soluble starch	1314:1327	H7 on soluble starch was estimated to be 13.73 mL/s/mg.
34064563	9	16	theme	statistical	1170:1180	arg1	approach					1182:1189	the statistical approach	1166:1189	the statistical approach	1166:1189	Statistical optimization gives the exact idea of variables that influence the production of enzymes, and hence, the statistical approach offers the best way to optimize the bioprocess.
34064563	10	17	theme	catalytic	1248:1256	arg1	efficiency					1258:1267	The high catalytic efficiency	1239:1267	The high catalytic efficiency (kcat/Km) of amylase from Bacillus sp	1239:1305	The high catalytic efficiency (kcat/Km) of amylase from Bacillus sp.
34064563	10	17	theme	catalytic	1248:1256	arg1	kcat/Km					1270:1276	kcat/Km	1270:1276	kcat/Km	1270:1276	The high catalytic efficiency (kcat/Km) of amylase from Bacillus sp.
34064563	11	18	theme	13.73	1349:1353	arg1	mL/s/mg					1355:1361	13.73 mL/s/mg	1349:1361	13.73 mL/s/mg	1349:1361	H7 on soluble starch was estimated to be 13.73 mL/s/mg.
34064563	3	19	theme	amylase-producing	384:400	arg1	cultures					402:409	potential amylase-producing cultures	374:409	potential amylase-producing cultures	374:409	This compels the availability of enzyme in greater yields that can be achieved by employing potential amylase-producing cultures and statistical optimization.
34064563	2	20	theme	Amylase	131:137	arg1	enzyme					152:157	Amylase (EC 3.2.1.1) enzyme	131:157	Amylase (EC 3.2.1.1) enzyme	131:157	Amylase (EC 3.2.1.1) enzyme has gained tremendous demand in various industries, including wastewater treatment, bioremediation and nano-biotechnology.
34064563	7	21	theme	response	839:846	arg1	RSM					869:871	RSM	869:871	RSM	869:871	The use of two-stage statistical optimization, i.e., PBD and response surface methodology (RSM), using central composite design (CCD) further improved the production of amylase.
34064563	7	21	theme	response	839:846	arg1	methodology					856:866	response surface methodology	839:866	response surface methodology (RSM)	839:872	The use of two-stage statistical optimization, i.e., PBD and response surface methodology (RSM), using central composite design (CCD) further improved the production of amylase.
34064563	0	22	theme	Statistical	0:10	arg1	Bioprocess					18:27	Statistical Based Bioprocess	0:27	Statistical Based Bioprocess	0:27	Statistical Based Bioprocess Design for Improved Production of Amylase from Halophilic Bacillus sp.
34064563	7	23	theme	statistical	799:809	arg1	optimization					811:822	two-stage statistical optimization	789:822	two-stage statistical optimization	789:822	The use of two-stage statistical optimization, i.e., PBD and response surface methodology (RSM), using central composite design (CCD) further improved the production of amylase.
34064563	2	24	theme	tremendous	170:179	arg1	demand					181:186	tremendous demand	170:186	tremendous demand in various industries, including wastewater treatment, bioremediation and nano-biotechnology	170:279	Amylase (EC 3.2.1.1) enzyme has gained tremendous demand in various industries, including wastewater treatment, bioremediation and nano-biotechnology.
34064563	10	25	theme	amylase	1282:1288	arg1	efficiency					1258:1267	The high catalytic efficiency	1239:1267	The high catalytic efficiency (kcat/Km) of amylase from Bacillus sp	1239:1305	The high catalytic efficiency (kcat/Km) of amylase from Bacillus sp.
34064563	10	25	theme	amylase	1282:1288	arg1	kcat/Km					1270:1276	kcat/Km	1270:1276	kcat/Km	1270:1276	The high catalytic efficiency (kcat/Km) of amylase from Bacillus sp.
34064563	10	26	from	sp	1304:1305	arg1	efficiency					1258:1267	The high catalytic efficiency	1239:1267	The high catalytic efficiency (kcat/Km) of amylase from Bacillus sp	1239:1305	The high catalytic efficiency (kcat/Km) of amylase from Bacillus sp.
34064563	10	26	from	sp	1304:1305	arg1	kcat/Km					1270:1276	kcat/Km	1270:1276	kcat/Km	1270:1276	The high catalytic efficiency (kcat/Km) of amylase from Bacillus sp.
34064563	7	27	theme	optimization	811:822	arg1	use					782:784	The use	778:784	The use of two-stage statistical optimization	778:822	The use of two-stage statistical optimization, i.e., PBD and response surface methodology (RSM), using central composite design (CCD) further improved the production of amylase.
34064563	5	28	theme	amylase-producing	693:709	arg1	strain					728:733	amylase-producing marine bacterial strain	693:733	amylase-producing marine bacterial strain identified as Bacillus sp	693:759	In the present work, we are reporting the screening of amylase-producing marine bacterial strain identified as Bacillus sp.
34064563	8	29	theme	amylase	980:986	arg1	production					988:997	amylase production	980:997	amylase production	980:997	A 1.31-fold increase in amylase production was evident using a 5.0 L laboratory-scale bioreactor.
34064563	9	30	theme	exact	1089:1093	arg1	idea					1095:1098	the exact idea	1085:1098	the exact idea of variables that influence the production of enzymes	1085:1152	Statistical optimization gives the exact idea of variables that influence the production of enzymes, and hence, the statistical approach offers the best way to optimize the bioprocess.
34064563	3	31	theme	potential	374:382	arg1	cultures					402:409	potential amylase-producing cultures	374:409	potential amylase-producing cultures	374:409	This compels the availability of enzyme in greater yields that can be achieved by employing potential amylase-producing cultures and statistical optimization.
34064563	5	32	theme	marine	711:716	arg1	strain					728:733	amylase-producing marine bacterial strain	693:733	amylase-producing marine bacterial strain identified as Bacillus sp	693:759	In the present work, we are reporting the screening of amylase-producing marine bacterial strain identified as Bacillus sp.
34064563	9	33	theme	best	1202:1205	arg1	way					1207:1209	the best way	1198:1209	the best way to optimize the bioprocess	1198:1236	Statistical optimization gives the exact idea of variables that influence the production of enzymes, and hence, the statistical approach offers the best way to optimize the bioprocess.
34064563	0	34	theme	Improved	40:47	arg1	Production					49:58	Improved Production	40:58	Improved Production of Amylase from Halophilic Bacillus sp	40:97	Statistical Based Bioprocess Design for Improved Production of Amylase from Halophilic Bacillus sp.
34064563	7	35	theme	two-stage	789:797	arg1	optimization					811:822	two-stage statistical optimization	789:822	two-stage statistical optimization	789:822	The use of two-stage statistical optimization, i.e., PBD and response surface methodology (RSM), using central composite design (CCD) further improved the production of amylase.
34064563	5	36	theme	bacterial	718:726	arg1	strain					728:733	amylase-producing marine bacterial strain	693:733	amylase-producing marine bacterial strain identified as Bacillus sp	693:759	In the present work, we are reporting the screening of amylase-producing marine bacterial strain identified as Bacillus sp.
34064563	4	37	theme	design	468:473	arg1	use					445:447	The use	441:447	The use of Plackett-Burman design (PBD) that evaluates various medium components and having two-level factorial designs	441:559	The use of Plackett-Burman design (PBD) that evaluates various medium components and having two-level factorial designs help to determine the factor and its level to increase the yield of product.
34064563	2	38	theme	wastewater	221:230	arg1	treatment					232:240	wastewater treatment	221:240	wastewater treatment	221:240	Amylase (EC 3.2.1.1) enzyme has gained tremendous demand in various industries, including wastewater treatment, bioremediation and nano-biotechnology.
34064563	5	39	theme	strain	728:733	arg1	screening					680:688	the screening	676:688	the screening of amylase-producing marine bacterial strain identified as Bacillus sp	676:759	In the present work, we are reporting the screening of amylase-producing marine bacterial strain identified as Bacillus sp.
34064563	9	40	theme	variables	1103:1111	arg1	idea					1095:1098	the exact idea	1085:1098	the exact idea of variables that influence the production of enzymes	1085:1152	Statistical optimization gives the exact idea of variables that influence the production of enzymes, and hence, the statistical approach offers the best way to optimize the bioprocess.
34064563	4	41	theme	factorial	543:551	arg1	designs					553:559	two-level factorial designs	533:559	two-level factorial designs	533:559	The use of Plackett-Burman design (PBD) that evaluates various medium components and having two-level factorial designs help to determine the factor and its level to increase the yield of product.
34064563	3	42	theme	statistical	415:425	arg1	optimization					427:438	statistical optimization	415:438	statistical optimization	415:438	This compels the availability of enzyme in greater yields that can be achieved by employing potential amylase-producing cultures and statistical optimization.
34064563	3	43	theme	enzyme	315:320	arg1	availability					299:310	the availability	295:310	the availability of enzyme	295:320	This compels the availability of enzyme in greater yields that can be achieved by employing potential amylase-producing cultures and statistical optimization.
34064563	2	44	theme	various	191:197	arg1	industries					199:208	various industries	191:208	various industries	191:208	Amylase (EC 3.2.1.1) enzyme has gained tremendous demand in various industries, including wastewater treatment, bioremediation and nano-biotechnology.
34064563	2	44	theme	various	191:197	arg1	bioremediation					243:256	bioremediation	243:256	bioremediation	243:256	Amylase (EC 3.2.1.1) enzyme has gained tremendous demand in various industries, including wastewater treatment, bioremediation and nano-biotechnology.
34064563	2	44	theme	various	191:197	arg1	nano-biotechnology					262:279	nano-biotechnology	262:279	nano-biotechnology	262:279	Amylase (EC 3.2.1.1) enzyme has gained tremendous demand in various industries, including wastewater treatment, bioremediation and nano-biotechnology.
34064563	2	44	theme	various	191:197	arg1	treatment					232:240	wastewater treatment	221:240	wastewater treatment	221:240	Amylase (EC 3.2.1.1) enzyme has gained tremendous demand in various industries, including wastewater treatment, bioremediation and nano-biotechnology.
34064563	10	45	theme	Bacillus	1295:1302	arg1	sp					1304:1305	Bacillus sp	1295:1305	Bacillus sp	1295:1305	The high catalytic efficiency (kcat/Km) of amylase from Bacillus sp.
34064563	1	46	theme	Marine	117:122	arg1	Water					124:128	Marine Water	117:128	Marine Water	117:128	H7 Isolated from Marine Water.
34064563	7	47	theme	composite	889:897	arg1	CCD					907:909	CCD	907:909	CCD	907:909	The use of two-stage statistical optimization, i.e., PBD and response surface methodology (RSM), using central composite design (CCD) further improved the production of amylase.
34064563	7	47	theme	composite	889:897	arg1	design					899:904	central composite design	881:904	central composite design (CCD)	881:910	The use of two-stage statistical optimization, i.e., PBD and response surface methodology (RSM), using central composite design (CCD) further improved the production of amylase.
34064563	9	48	theme	enzymes	1146:1152	arg1	production					1132:1141	the production	1128:1141	the production of enzymes	1128:1152	Statistical optimization gives the exact idea of variables that influence the production of enzymes, and hence, the statistical approach offers the best way to optimize the bioprocess.
34064563	4	49	theme	Plackett-Burman	452:466	arg1	PBD					476:478	PBD	476:478	PBD	476:478	The use of Plackett-Burman design (PBD) that evaluates various medium components and having two-level factorial designs help to determine the factor and its level to increase the yield of product.
34064563	4	49	theme	Plackett-Burman	452:466	arg1	design					468:473	Plackett-Burman design	452:473	Plackett-Burman design (PBD) that evaluates various medium components and having two-level factorial designs	452:559	The use of Plackett-Burman design (PBD) that evaluates various medium components and having two-level factorial designs help to determine the factor and its level to increase the yield of product.
34064563	10	50	theme	high	1243:1246	arg1	efficiency					1258:1267	The high catalytic efficiency	1239:1267	The high catalytic efficiency (kcat/Km) of amylase from Bacillus sp	1239:1305	The high catalytic efficiency (kcat/Km) of amylase from Bacillus sp.
34064563	10	50	theme	high	1243:1246	arg1	kcat/Km					1270:1276	kcat/Km	1270:1276	kcat/Km	1270:1276	The high catalytic efficiency (kcat/Km) of amylase from Bacillus sp.
34064563	0	51	from	sp	96:97	arg1	Production					49:58	Improved Production	40:58	Improved Production of Amylase from Halophilic Bacillus sp	40:97	Statistical Based Bioprocess Design for Improved Production of Amylase from Halophilic Bacillus sp.
34064563	3	52	theme	greater	325:331	arg1	yields					333:338	greater yields	325:338	greater yields that can be achieved by employing potential amylase-producing cultures and statistical optimization	325:438	This compels the availability of enzyme in greater yields that can be achieved by employing potential amylase-producing cultures and statistical optimization.
34064563	7	53	theme	central	881:887	arg1	CCD					907:909	CCD	907:909	CCD	907:909	The use of two-stage statistical optimization, i.e., PBD and response surface methodology (RSM), using central composite design (CCD) further improved the production of amylase.
34064563	7	53	theme	central	881:887	arg1	design					899:904	central composite design	881:904	central composite design (CCD)	881:910	The use of two-stage statistical optimization, i.e., PBD and response surface methodology (RSM), using central composite design (CCD) further improved the production of amylase.
34064563	4	54	theme	product	629:635	arg1	yield					620:624	the yield	616:624	the yield of product	616:635	The use of Plackett-Burman design (PBD) that evaluates various medium components and having two-level factorial designs help to determine the factor and its level to increase the yield of product.
34064563	0	55	theme	Amylase	63:69	arg1	Production					49:58	Improved Production	40:58	Improved Production of Amylase from Halophilic Bacillus sp	40:97	Statistical Based Bioprocess Design for Improved Production of Amylase from Halophilic Bacillus sp.
34064563	11	56	from	H7	1308:1309	arg1	starch					1322:1327	soluble starch	1314:1327	soluble starch	1314:1327	H7 on soluble starch was estimated to be 13.73 mL/s/mg.
34064563	7	57	theme	amylase	947:953	arg1	production					933:942	the production	929:942	the production of amylase	929:953	The use of two-stage statistical optimization, i.e., PBD and response surface methodology (RSM), using central composite design (CCD) further improved the production of amylase.
34064563	0	58	theme	Based	12:16	arg1	Bioprocess					18:27	Statistical Based Bioprocess	0:27	Statistical Based Bioprocess	0:27	Statistical Based Bioprocess Design for Improved Production of Amylase from Halophilic Bacillus sp.
34064563	5	59	theme	present	645:651	arg1	work					653:656	the present work	641:656	the present work	641:656	In the present work, we are reporting the screening of amylase-producing marine bacterial strain identified as Bacillus sp.
32885670	0	0	theme	metabolites	61:71	arg1	δ13C					47:50	δ13C	47:50	δ13C of plant metabolites	47:71	Nitrate and ammonium differ in their impact on δ13C of plant metabolites and respired CO2 from tobacco leaves.
32885670	8	1	theme	13C	1091:1093	arg1	enrichment					1095:1104	intra-molecular 13C enrichment	1075:1104	intra-molecular 13C enrichment in malate	1075:1114	Together with estimates of intra-molecular 13C enrichment in malate, we conclude that a change in the anaplerotic reaction of the TCA cycle possibly contributes to 13C enrichment in organic acids and respired CO2 under supply.
32885670	9	2	from	effect	1285:1290	arg1	δ13C					1302:1305	δ13C	1302:1305	δ13C	1302:1305	Thus, the effect of vs. on δ13C is highly relevant, particularly if δ13C of leaf metabolites or respiration is used as proxy for plant performance.
32885670	8	3	from	change	1136:1141	arg1	reaction					1162:1169	the anaplerotic reaction	1146:1169	the anaplerotic reaction of the TCA cycle	1146:1186	Together with estimates of intra-molecular 13C enrichment in malate, we conclude that a change in the anaplerotic reaction of the TCA cycle possibly contributes to 13C enrichment in organic acids and respired CO2 under supply.
32885670	8	4	theme	enrichment	1095:1104	arg1	estimates					1062:1070	estimates	1062:1070	estimates of intra-molecular 13C enrichment in malate	1062:1114	Together with estimates of intra-molecular 13C enrichment in malate, we conclude that a change in the anaplerotic reaction of the TCA cycle possibly contributes to 13C enrichment in organic acids and respired CO2 under supply.
32885670	0	5	theme	plant	55:59	arg1	metabolites					61:71	plant metabolites	55:71	plant metabolites	55:71	Nitrate and ammonium differ in their impact on δ13C of plant metabolites and respired CO2 from tobacco leaves.
32885670	9	6	theme	leaf	1351:1354	arg1	metabolites					1356:1366	leaf metabolites	1351:1366	leaf metabolites	1351:1366	Thus, the effect of vs. on δ13C is highly relevant, particularly if δ13C of leaf metabolites or respiration is used as proxy for plant performance.
32885670	9	7	used	used	1386:1389	arg2	δ13C					1343:1346	δ13C	1343:1346	δ13C of leaf metabolites or respiration	1343:1381	Thus, the effect of vs. on δ13C is highly relevant, particularly if δ13C of leaf metabolites or respiration is used as proxy for plant performance.
32885670	9	7	used	used	1386:1389	arg2	proxy					1394:1398	proxy	1394:1398	proxy for plant performance	1394:1420	Thus, the effect of vs. on δ13C is highly relevant, particularly if δ13C of leaf metabolites or respiration is used as proxy for plant performance.
32885670	0	8	dep	tobacco	95:101	arg1	leaves					103:108	leaves	103:108	leaves	103:108	Nitrate and ammonium differ in their impact on δ13C of plant metabolites and respired CO2 from tobacco leaves.
32885670	8	9	from	enrichment	1095:1104	arg1	malate					1109:1114	malate	1109:1114	malate	1109:1114	Together with estimates of intra-molecular 13C enrichment in malate, we conclude that a change in the anaplerotic reaction of the TCA cycle possibly contributes to 13C enrichment in organic acids and respired CO2 under supply.
32885670	2	10	theme	supply	234:239	arg1	effect					220:225	the effect	216:225	the effect of vs. supply on δ13C of leaf metabolites and respired CO2	216:284	However, the effect of vs. supply on δ13C of leaf metabolites and respired CO2 is largely unknown.
32885670	2	10	theme	supply	234:239	arg1	unknown					297:303	unknown	297:303	unknown	297:303	However, the effect of vs. supply on δ13C of leaf metabolites and respired CO2 is largely unknown.
32885670	4	11	theme	acids	679:683	arg1	rate					631:634	Net assimilation rate	614:634	Net assimilation rate	614:634	Net assimilation rate, dry biomass and concentrations of organic acids and starch decreased along the gradient.
32885670	4	11	theme	acids	679:683	arg1	biomass					641:647	dry biomass	637:647	dry biomass	637:647	Net assimilation rate, dry biomass and concentrations of organic acids and starch decreased along the gradient.
32885670	4	11	theme	acids	679:683	arg1	concentrations					653:666	concentrations	653:666	concentrations	653:666	Net assimilation rate, dry biomass and concentrations of organic acids and starch decreased along the gradient.
32885670	4	12	theme	organic	671:677	arg1	acids					679:683	organic acids	671:683	organic acids	671:683	Net assimilation rate, dry biomass and concentrations of organic acids and starch decreased along the gradient.
32885670	4	13	theme	starch	689:694	arg1	rate					631:634	Net assimilation rate	614:634	Net assimilation rate	614:634	Net assimilation rate, dry biomass and concentrations of organic acids and starch decreased along the gradient.
32885670	4	13	theme	starch	689:694	arg1	biomass					641:647	dry biomass	637:647	dry biomass	637:647	Net assimilation rate, dry biomass and concentrations of organic acids and starch decreased along the gradient.
32885670	4	13	theme	starch	689:694	arg1	concentrations					653:666	concentrations	653:666	concentrations	653:666	Net assimilation rate, dry biomass and concentrations of organic acids and starch decreased along the gradient.
32885670	8	14	from	enrichment	1216:1225	arg1	acids					1238:1242	organic acids	1230:1242	organic acids	1230:1242	Together with estimates of intra-molecular 13C enrichment in malate, we conclude that a change in the anaplerotic reaction of the TCA cycle possibly contributes to 13C enrichment in organic acids and respired CO2 under supply.
32885670	3	15	theme	intermediates	473:485	arg1	δ13C					434:437	δ13C	434:437	δ13C	434:437	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	3	15	theme	intermediates	473:485	arg1	δ13C					488:491	δ13C	488:491	δ13C of dark-respired CO2	488:512	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	3	15	theme	intermediates	473:485	arg1	activities					519:528	activities	519:528	activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase	519:611	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	3	15	theme	intermediates	473:485	arg1	concentrations					415:428	concentrations	415:428	concentrations	415:428	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	3	15	theme	intermediates	473:485	arg1	variables					404:412	gas exchange variables	391:412	gas exchange variables	391:412	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	0	16	from	impact	37:42	arg1	δ13C					47:50	δ13C	47:50	δ13C of plant metabolites	47:71	Nitrate and ammonium differ in their impact on δ13C of plant metabolites and respired CO2 from tobacco leaves.
32885670	8	17	theme	13C	1212:1214	arg1	enrichment					1216:1225	13C enrichment	1212:1225	13C enrichment in organic acids	1212:1242	Together with estimates of intra-molecular 13C enrichment in malate, we conclude that a change in the anaplerotic reaction of the TCA cycle possibly contributes to 13C enrichment in organic acids and respired CO2 under supply.
32885670	3	18	theme	TCA	462:464	arg1	intermediates					473:485	tricarboxylic acid (TCA) cycle intermediates	442:485	tricarboxylic acid (TCA) cycle intermediates	442:485	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	9	19	theme	metabolites	1356:1366	arg1	proxy					1394:1398	proxy	1394:1398	proxy for plant performance	1394:1420	Thus, the effect of vs. on δ13C is highly relevant, particularly if δ13C of leaf metabolites or respiration is used as proxy for plant performance.
32885670	9	19	theme	metabolites	1356:1366	arg1	δ13C					1343:1346	δ13C	1343:1346	δ13C of leaf metabolites or respiration	1343:1381	Thus, the effect of vs. on δ13C is highly relevant, particularly if δ13C of leaf metabolites or respiration is used as proxy for plant performance.
32885670	9	20	theme	plant	1404:1408	arg1	performance					1410:1420	plant performance	1404:1420	plant performance	1404:1420	Thus, the effect of vs. on δ13C is highly relevant, particularly if δ13C of leaf metabolites or respiration is used as proxy for plant performance.
32885670	5	21	theme	soluble	795:801	arg1	rates					751:755	respiration rates	739:755	respiration rates	739:755	In contrast, respiration rates, concentrations of intercellular CO2, soluble sugars and amino acids increased.
32885670	5	21	theme	soluble	795:801	arg1	sugars					803:808	soluble sugars	795:808	soluble sugars	795:808	In contrast, respiration rates, concentrations of intercellular CO2, soluble sugars and amino acids increased.
32885670	3	22	theme	tobacco	318:324	arg1	plants					326:331	tobacco plants	318:331	tobacco plants	318:331	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	8	23	theme	intra-molecular	1075:1089	arg1	enrichment					1095:1104	intra-molecular 13C enrichment	1075:1104	intra-molecular 13C enrichment in malate	1075:1114	Together with estimates of intra-molecular 13C enrichment in malate, we conclude that a change in the anaplerotic reaction of the TCA cycle possibly contributes to 13C enrichment in organic acids and respired CO2 under supply.
32885670	2	24	theme	leaf	252:255	arg1	metabolites					257:267	leaf metabolites	252:267	leaf metabolites	252:267	However, the effect of vs. supply on δ13C of leaf metabolites and respired CO2 is largely unknown.
32885670	8	25	theme	TCA	1178:1180	arg1	cycle					1182:1186	the TCA cycle	1174:1186	the TCA cycle	1174:1186	Together with estimates of intra-molecular 13C enrichment in malate, we conclude that a change in the anaplerotic reaction of the TCA cycle possibly contributes to 13C enrichment in organic acids and respired CO2 under supply.
32885670	5	26	theme	respiration	739:749	arg1	sugars					803:808	soluble sugars	795:808	soluble sugars	795:808	In contrast, respiration rates, concentrations of intercellular CO2, soluble sugars and amino acids increased.
32885670	5	26	theme	respiration	739:749	arg1	concentrations					758:771	concentrations	758:771	concentrations of intercellular CO2	758:792	In contrast, respiration rates, concentrations of intercellular CO2, soluble sugars and amino acids increased.
32885670	5	26	theme	respiration	739:749	arg1	rates					751:755	respiration rates	739:755	respiration rates	739:755	In contrast, respiration rates, concentrations of intercellular CO2, soluble sugars and amino acids increased.
32885670	5	26	theme	respiration	739:749	arg1	acids					820:824	amino acids	814:824	amino acids	814:824	In contrast, respiration rates, concentrations of intercellular CO2, soluble sugars and amino acids increased.
32885670	7	27	theme	potential	1010:1018	arg1	substrates					1020:1029	potential substrates	1010:1029	potential substrates for respiration	1010:1045	δ13C of CO2 and organic acids closely co-varied and were more positive under supply, suggesting organic acids as potential substrates for respiration.
32885670	7	27	theme	potential	1010:1018	arg1	acids					1001:1005	organic acids	993:1005	organic acids	993:1005	δ13C of CO2 and organic acids closely co-varied and were more positive under supply, suggesting organic acids as potential substrates for respiration.
32885670	3	28	theme	dark-respired	496:508	arg1	CO2					510:512	dark-respired CO2	496:512	dark-respired CO2	496:512	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	8	29	theme	cycle	1182:1186	arg1	reaction					1162:1169	the anaplerotic reaction	1146:1169	the anaplerotic reaction of the TCA cycle	1146:1186	Together with estimates of intra-molecular 13C enrichment in malate, we conclude that a change in the anaplerotic reaction of the TCA cycle possibly contributes to 13C enrichment in organic acids and respired CO2 under supply.
32885670	3	30	theme	concentration	355:367	arg1	ratios					369:374	concentration ratios	355:374	concentration ratios	355:374	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	7	31	theme	organic	993:999	arg1	substrates					1020:1029	potential substrates	1010:1029	potential substrates for respiration	1010:1045	δ13C of CO2 and organic acids closely co-varied and were more positive under supply, suggesting organic acids as potential substrates for respiration.
32885670	7	31	theme	organic	993:999	arg1	acids					1001:1005	organic acids	993:1005	organic acids	993:1005	δ13C of CO2 and organic acids closely co-varied and were more positive under supply, suggesting organic acids as potential substrates for respiration.
32885670	3	32	theme	exchange	395:402	arg1	variables					404:412	gas exchange variables	391:412	gas exchange variables	391:412	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	9	33	theme	respiration	1371:1381	arg1	proxy					1394:1398	proxy	1394:1398	proxy for plant performance	1394:1420	Thus, the effect of vs. on δ13C is highly relevant, particularly if δ13C of leaf metabolites or respiration is used as proxy for plant performance.
32885670	9	33	theme	respiration	1371:1381	arg1	δ13C					1343:1346	δ13C	1343:1346	δ13C of leaf metabolites or respiration	1343:1381	Thus, the effect of vs. on δ13C is highly relevant, particularly if δ13C of leaf metabolites or respiration is used as proxy for plant performance.
32885670	3	34	theme	malic	564:568	arg1	enzyme					570:575	malic enzyme	564:575	malic enzyme	564:575	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	3	34	theme	malic	564:568	arg1	enzymes					537:543	key enzymes	533:543	key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase	533:611	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	3	35	theme	tricarboxylic	442:454	arg1	intermediates					473:485	tricarboxylic acid (TCA) cycle intermediates	442:485	tricarboxylic acid (TCA) cycle intermediates	442:485	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	1	36	theme	carbon	115:120	arg1	δ13C					144:147	δ13C	144:147	δ13C	144:147	The carbon isotopic composition (δ13C) of foliage is often used as proxy for plant performance.
32885670	1	36	theme	carbon	115:120	arg1	composition					131:141	The carbon isotopic composition	111:141	The carbon isotopic composition (δ13C) of foliage	111:159	The carbon isotopic composition (δ13C) of foliage is often used as proxy for plant performance.
32885670	1	36	theme	carbon	115:120	arg1	proxy					178:182	proxy	178:182	proxy for plant performance	178:204	The carbon isotopic composition (δ13C) of foliage is often used as proxy for plant performance.
32885670	4	37	theme	dry	637:639	arg1	biomass					641:647	dry biomass	637:647	dry biomass	637:647	Net assimilation rate, dry biomass and concentrations of organic acids and starch decreased along the gradient.
32885670	5	38	theme	intercellular	776:788	arg1	CO2					790:792	intercellular CO2	776:792	intercellular CO2	776:792	In contrast, respiration rates, concentrations of intercellular CO2, soluble sugars and amino acids increased.
32885670	1	39	theme	foliage	153:159	arg1	δ13C					144:147	δ13C	144:147	δ13C	144:147	The carbon isotopic composition (δ13C) of foliage is often used as proxy for plant performance.
32885670	1	39	theme	foliage	153:159	arg1	composition					131:141	The carbon isotopic composition	111:141	The carbon isotopic composition (δ13C) of foliage	111:159	The carbon isotopic composition (δ13C) of foliage is often used as proxy for plant performance.
32885670	1	39	theme	foliage	153:159	arg1	proxy					178:182	proxy	178:182	proxy for plant performance	178:204	The carbon isotopic composition (δ13C) of foliage is often used as proxy for plant performance.
32885670	3	40	dep	enzymes	537:543	arg1	enzymes					537:543	key enzymes	533:543	key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase	533:611	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	3	40	dep	enzymes	537:543	arg1	reductase					553:561	nitrate reductase	545:561	nitrate reductase	545:561	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	3	40	dep	enzymes	537:543	arg1	enzyme					570:575	malic enzyme	564:575	malic enzyme	564:575	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	3	40	dep	enzymes	537:543	arg1	carboxylase					601:611	phosphoenolpyruvate carboxylase	581:611	phosphoenolpyruvate carboxylase	581:611	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	4	41	theme	assimilation	618:629	arg1	rate					631:634	Net assimilation rate	614:634	Net assimilation rate	614:634	Net assimilation rate, dry biomass and concentrations of organic acids and starch decreased along the gradient.
32885670	6	42	theme	enzymes	878:884	arg1	activities					851:860	activities	851:860	activities of all measured enzymes	851:884	As decreased, activities of all measured enzymes decreased.
32885670	1	43	used	used	170:173	arg2	δ13C					144:147	δ13C	144:147	δ13C	144:147	The carbon isotopic composition (δ13C) of foliage is often used as proxy for plant performance.
32885670	1	43	used	used	170:173	arg2	composition					131:141	The carbon isotopic composition	111:141	The carbon isotopic composition (δ13C) of foliage	111:159	The carbon isotopic composition (δ13C) of foliage is often used as proxy for plant performance.
32885670	1	43	used	used	170:173	arg2	proxy					178:182	proxy	178:182	proxy for plant performance	178:204	The carbon isotopic composition (δ13C) of foliage is often used as proxy for plant performance.
32885670	5	44	theme	CO2	790:792	arg1	concentrations					758:771	concentrations	758:771	concentrations of intercellular CO2	758:792	In contrast, respiration rates, concentrations of intercellular CO2, soluble sugars and amino acids increased.
32885670	5	44	theme	CO2	790:792	arg1	rates					751:755	respiration rates	739:755	respiration rates	739:755	In contrast, respiration rates, concentrations of intercellular CO2, soluble sugars and amino acids increased.
32885670	3	45	theme	gas	391:393	arg1	variables					404:412	gas exchange variables	391:412	gas exchange variables	391:412	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	8	46	theme	anaplerotic	1150:1160	arg1	reaction					1162:1169	the anaplerotic reaction	1146:1169	the anaplerotic reaction of the TCA cycle	1146:1186	Together with estimates of intra-molecular 13C enrichment in malate, we conclude that a change in the anaplerotic reaction of the TCA cycle possibly contributes to 13C enrichment in organic acids and respired CO2 under supply.
32885670	5	47	theme	amino	814:818	arg1	rates					751:755	respiration rates	739:755	respiration rates	739:755	In contrast, respiration rates, concentrations of intercellular CO2, soluble sugars and amino acids increased.
32885670	5	47	theme	amino	814:818	arg1	acids					820:824	amino acids	814:824	amino acids	814:824	In contrast, respiration rates, concentrations of intercellular CO2, soluble sugars and amino acids increased.
32885670	8	48	from	estimates	1062:1070	arg1	malate					1109:1114	malate	1109:1114	malate	1109:1114	Together with estimates of intra-molecular 13C enrichment in malate, we conclude that a change in the anaplerotic reaction of the TCA cycle possibly contributes to 13C enrichment in organic acids and respired CO2 under supply.
32885670	3	49	theme	CO2	510:512	arg1	δ13C					434:437	δ13C	434:437	δ13C	434:437	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	3	49	theme	CO2	510:512	arg1	δ13C					488:491	δ13C	488:491	δ13C of dark-respired CO2	488:512	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	3	49	theme	CO2	510:512	arg1	activities					519:528	activities	519:528	activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase	519:611	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	3	49	theme	CO2	510:512	arg1	concentrations					415:428	concentrations	415:428	concentrations	415:428	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	3	49	theme	CO2	510:512	arg1	variables					404:412	gas exchange variables	391:412	gas exchange variables	391:412	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	2	50	theme	respired	273:280	arg1	CO2					282:284	respired CO2	273:284	respired CO2	273:284	However, the effect of vs. supply on δ13C of leaf metabolites and respired CO2 is largely unknown.
32885670	8	51	from	malate	1109:1114	arg1	estimates					1062:1070	estimates	1062:1070	estimates of intra-molecular 13C enrichment in malate	1062:1114	Together with estimates of intra-molecular 13C enrichment in malate, we conclude that a change in the anaplerotic reaction of the TCA cycle possibly contributes to 13C enrichment in organic acids and respired CO2 under supply.
32885670	3	52	theme	enzymes	537:543	arg1	δ13C					434:437	δ13C	434:437	δ13C	434:437	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	3	52	theme	enzymes	537:543	arg1	δ13C					488:491	δ13C	488:491	δ13C of dark-respired CO2	488:512	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	3	52	theme	enzymes	537:543	arg1	activities					519:528	activities	519:528	activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase	519:611	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	3	52	theme	enzymes	537:543	arg1	concentrations					415:428	concentrations	415:428	concentrations	415:428	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	3	52	theme	enzymes	537:543	arg1	variables					404:412	gas exchange variables	391:412	gas exchange variables	391:412	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	4	53	theme	Net	614:616	arg1	rate					631:634	Net assimilation rate	614:634	Net assimilation rate	614:634	Net assimilation rate, dry biomass and concentrations of organic acids and starch decreased along the gradient.
32885670	8	54	theme	organic	1230:1236	arg1	acids					1238:1242	organic acids	1230:1242	organic acids	1230:1242	Together with estimates of intra-molecular 13C enrichment in malate, we conclude that a change in the anaplerotic reaction of the TCA cycle possibly contributes to 13C enrichment in organic acids and respired CO2 under supply.
32885670	2	55	from	effect	220:225	arg1	δ13C					244:247	δ13C	244:247	δ13C of leaf metabolites	244:267	However, the effect of vs. supply on δ13C of leaf metabolites and respired CO2 is largely unknown.
32885670	2	55	from	effect	220:225	arg1	CO2					282:284	respired CO2	273:284	respired CO2	273:284	However, the effect of vs. supply on δ13C of leaf metabolites and respired CO2 is largely unknown.
32885670	9	56	theme	vs.	1295:1297	arg1	relevant					1317:1324	relevant	1317:1324	relevant	1317:1324	Thus, the effect of vs. on δ13C is highly relevant, particularly if δ13C of leaf metabolites or respiration is used as proxy for plant performance.
32885670	9	56	theme	vs.	1295:1297	arg1	effect					1285:1290	the effect	1281:1290	the effect of vs. on δ13C	1281:1305	Thus, the effect of vs. on δ13C is highly relevant, particularly if δ13C of leaf metabolites or respiration is used as proxy for plant performance.
32885670	3	57	theme	nitrate	545:551	arg1	reductase					553:561	nitrate reductase	545:561	nitrate reductase	545:561	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	3	57	theme	nitrate	545:551	arg1	enzymes					537:543	key enzymes	533:543	key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase	533:611	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	7	58	theme	organic	913:919	arg1	acids					921:925	organic acids	913:925	organic acids	913:925	δ13C of CO2 and organic acids closely co-varied and were more positive under supply, suggesting organic acids as potential substrates for respiration.
32885670	2	59	theme	metabolites	257:267	arg1	δ13C					244:247	δ13C	244:247	δ13C of leaf metabolites	244:267	However, the effect of vs. supply on δ13C of leaf metabolites and respired CO2 is largely unknown.
32885670	2	59	theme	metabolites	257:267	arg1	CO2					282:284	respired CO2	273:284	respired CO2	273:284	However, the effect of vs. supply on δ13C of leaf metabolites and respired CO2 is largely unknown.
32885670	3	60	theme	phosphoenolpyruvate	581:599	arg1	enzymes					537:543	key enzymes	533:543	key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase	533:611	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	3	60	theme	phosphoenolpyruvate	581:599	arg1	carboxylase					601:611	phosphoenolpyruvate carboxylase	581:611	phosphoenolpyruvate carboxylase	581:611	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	7	61	theme	acids	921:925	arg1	δ13C					897:900	δ13C	897:900	δ13C of CO2 and organic acids	897:925	δ13C of CO2 and organic acids closely co-varied and were more positive under supply, suggesting organic acids as potential substrates for respiration.
32885670	3	62	theme	acid	456:459	arg1	intermediates					473:485	tricarboxylic acid (TCA) cycle intermediates	442:485	tricarboxylic acid (TCA) cycle intermediates	442:485	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	1	63	theme	plant	188:192	arg1	performance					194:204	plant performance	188:204	plant performance	188:204	The carbon isotopic composition (δ13C) of foliage is often used as proxy for plant performance.
32885670	3	64	theme	cycle	467:471	arg1	intermediates					473:485	tricarboxylic acid (TCA) cycle intermediates	442:485	tricarboxylic acid (TCA) cycle intermediates	442:485	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	3	65	theme	key	533:535	arg1	carboxylase					601:611	phosphoenolpyruvate carboxylase	581:611	phosphoenolpyruvate carboxylase	581:611	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	3	65	theme	key	533:535	arg1	reductase					553:561	nitrate reductase	545:561	nitrate reductase	545:561	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	3	65	theme	key	533:535	arg1	enzyme					570:575	malic enzyme	564:575	malic enzyme	564:575	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	3	65	theme	key	533:535	arg1	enzymes					537:543	key enzymes	533:543	key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase	533:611	We supplied tobacco plants with a gradient of to concentration ratios and determined gas exchange variables, concentrations and δ13C of tricarboxylic acid (TCA) cycle intermediates, δ13C of dark-respired CO2, and activities of key enzymes nitrate reductase, malic enzyme and phosphoenolpyruvate carboxylase.
32885670	7	66	theme	CO2	905:907	arg1	δ13C					897:900	δ13C	897:900	δ13C of CO2 and organic acids	897:925	δ13C of CO2 and organic acids closely co-varied and were more positive under supply, suggesting organic acids as potential substrates for respiration.
32885670	1	67	theme	isotopic	122:129	arg1	δ13C					144:147	δ13C	144:147	δ13C	144:147	The carbon isotopic composition (δ13C) of foliage is often used as proxy for plant performance.
32885670	1	67	theme	isotopic	122:129	arg1	composition					131:141	The carbon isotopic composition	111:141	The carbon isotopic composition (δ13C) of foliage	111:159	The carbon isotopic composition (δ13C) of foliage is often used as proxy for plant performance.
32885670	1	67	theme	isotopic	122:129	arg1	proxy					178:182	proxy	178:182	proxy for plant performance	178:204	The carbon isotopic composition (δ13C) of foliage is often used as proxy for plant performance.
32885670	6	68	theme	measured	869:876	arg1	enzymes					878:884	all measured enzymes	865:884	all measured enzymes	865:884	As decreased, activities of all measured enzymes decreased.
33806219	7	0	theme	±	1492:1492	arg1	g/cm3					1498:1502	14.6 ± 0.7 g/cm3	1487:1502	14.6 ± 0.7 g/cm3	1487:1502	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	4	1	theme	materials	913:921	arg1	cytocompatibility					879:895	the cytocompatibility	875:895	the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells	875:954	Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells.
33806219	7	2	theme	swelling	1279:1286	arg1	ability					1288:1294	higher swelling ability	1272:1294	higher swelling ability	1272:1294	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	3	3	theme	infrared	502:509	arg1	spectroscopy					511:522	Fourier transfer infrared spectroscopy	485:522	Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations	485:659	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	4	4	theme	MG-63	928:932	arg1	cells					950:954	MG-63 osteoblast-like cells	928:954	MG-63 osteoblast-like cells	928:954	Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells.
33806219	7	5	from	%	1670:1670	arg1	range					1649:1653	the range	1645:1653	the range of 12.67 ±	1645:1664	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	3	6	theme	device	558:563	arg1	measurements					613:624	attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements	529:624	attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements	529:624	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	6	7	theme	biopolymeric	1194:1205	arg1	scaffolds					1207:1215	biopolymeric scaffolds	1194:1215	biopolymeric scaffolds	1194:1215	It was found that DAS and DAC have different influence on the properties of biopolymeric scaffolds.
33806219	9	8	theme	obtained	1868:1875	arg1	results					1877:1883	the obtained results	1864:1883	the obtained results	1864:1883	However, the obtained results showed that both types of scaffolds can provide the support required in regenerative medicine and tissue engineering.
33806219	3	9	theme	mechanical	706:715	arg1	properties					717:726	mechanical properties	706:726	mechanical properties evaluation	706:737	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	3	10	theme	total	540:544	arg1	FTIR-ATR					566:573	FTIR-ATR	566:573	FTIR-ATR	566:573	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	3	10	theme	total	540:544	arg1	device					558:563	total reflectance device	540:563	total reflectance device (FTIR-ATR)	540:574	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	7	11	dep	density	1391:1397	arg1	cross-linked					1464:1475	cross-linked	1464:1475	cross-linked	1464:1475	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	7	12	link	cross-linked	1351:1362	arg1	materials					1364:1372	DAC cross-linked materials	1347:1372	DAC cross-linked materials	1347:1372	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	1	13	theme	scaffolds	188:196	arg1	blends					243:248	collagen and chitosan blends	221:248	collagen and chitosan blends	221:248	The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC).
33806219	1	13	theme	scaffolds	188:196	arg1	properties					156:165	physicochemical properties	140:165	physicochemical properties of three dimensional scaffolds based on silk fibroin	140:218	The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC).
33806219	3	14	theme	thermogravimetric	743:759	arg1	analysis					761:768	thermogravimetric analysis	743:768	thermogravimetric analysis	743:768	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	7	15	theme	cross-linked	1612:1623	arg1	scaffolds					1625:1633	DAS cross-linked scaffolds	1608:1633	DAS cross-linked scaffolds	1608:1633	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	3	16	theme	water	599:603	arg1	content					605:611	water content	599:611	water content	599:611	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	7	17	theme	lower	1385:1389	arg1	density					1391:1397	lower density	1385:1397	lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3)	1385:1503	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	10	18	theme	defects	2134:2140	arg1	healing					2112:2118	healing	2112:2118	healing of small bone defects	2112:2140	The scaffolds presented in the present work can be potentially used in bone tissue engineering to facilitate healing of small bone defects.
33806219	5	19	theme	chemical	1077:1084	arg1	cross-linking					1086:1098	chemical cross-linking	1077:1098	chemical cross-linking with DAS and DAC	1077:1115	The results showed that the properties of the scaffolds based on silk fibroin, collagen and chitosan can be modified by chemical cross-linking with DAS and DAC.
33806219	3	20	theme	behavior	586:593	arg1	measurements					613:624	attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements	529:624	attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements	529:624	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	10	21	theme	tissue	2079:2084	arg1	engineering					2086:2096	bone tissue engineering	2074:2096	bone tissue engineering	2074:2096	The scaffolds presented in the present work can be potentially used in bone tissue engineering to facilitate healing of small bone defects.
33806219	10	22	theme	small	2123:2127	arg1	defects					2134:2140	small bone defects	2123:2140	small bone defects	2123:2140	The scaffolds presented in the present work can be potentially used in bone tissue engineering to facilitate healing of small bone defects.
33806219	7	23	link	cross-linked	1612:1623	arg1	scaffolds					1625:1633	DAS cross-linked scaffolds	1608:1633	DAS cross-linked scaffolds	1608:1633	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	7	24	theme	higher	1272:1277	arg1	ability					1288:1294	higher swelling ability	1272:1294	higher swelling ability	1272:1294	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	7	25	theme	19.83	1676:1680	arg1	%					1688:1688	19.83 ± 1.30%	1676:1688	19.83 ± 1.30%	1676:1688	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	9	26	theme	regenerative	1957:1968	arg1	medicine					1970:1977	regenerative medicine	1957:1977	regenerative medicine	1957:1977	However, the obtained results showed that both types of scaffolds can provide the support required in regenerative medicine and tissue engineering.
33806219	4	27	with	cytocompatibility	879:895	arg1	cells					950:954	MG-63 osteoblast-like cells	928:954	MG-63 osteoblast-like cells	928:954	Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells.
33806219	7	28	theme	DAC	1562:1564	arg1	scaffolds					1579:1587	DAC cross-linked scaffolds	1562:1587	DAC cross-linked scaffolds	1562:1587	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	0	29	theme	Materials	93:101	arg1	Properties					66:75	Physicochemical Properties	50:75	Physicochemical Properties of Biopolymeric Materials	50:101	Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials?
33806219	6	30	theme	scaffolds	1207:1215	arg1	properties					1180:1189	the properties	1176:1189	the properties of biopolymeric scaffolds	1176:1215	It was found that DAS and DAC have different influence on the properties of biopolymeric scaffolds.
33806219	7	31	theme	±	1664:1664	arg1	range					1649:1653	the range	1645:1653	the range of 12.67 ±	1645:1664	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	2	32	theme	one-step	382:389	arg1	synthesis					391:399	one-step synthesis	382:399	one-step synthesis	382:399	DAS was commercially available, while DAC was obtained by one-step synthesis.
33806219	5	33	with	cross-linking	1086:1098	arg1	DAC					1113:1115	DAC	1113:1115	DAC	1113:1115	The results showed that the properties of the scaffolds based on silk fibroin, collagen and chitosan can be modified by chemical cross-linking with DAS and DAC.
33806219	5	33	with	cross-linking	1086:1098	arg1	DAS					1105:1107	DAS	1105:1107	DAS	1105:1107	The results showed that the properties of the scaffolds based on silk fibroin, collagen and chitosan can be modified by chemical cross-linking with DAS and DAC.
33806219	1	34	theme	dialdehyde	269:278	arg1	DAS					288:290	DAS	288:290	DAS	288:290	The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC).
33806219	1	34	theme	dialdehyde	269:278	arg1	starch					280:285	dialdehyde starch	269:285	dialdehyde starch (DAS)	269:291	The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC).
33806219	7	35	theme	DAC	1347:1349	arg1	materials					1364:1372	DAC cross-linked materials	1347:1372	DAC cross-linked materials	1347:1372	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	4	36	theme	Metabolic	771:779	arg1	activity					781:788	Metabolic activity	771:788	Metabolic activity with AlamarBlue assay and live/dead fluorescence staining	771:846	Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells.
33806219	5	37	theme	scaffolds	1003:1011	arg1	properties					985:994	the properties	981:994	the properties of the scaffolds based on silk fibroin, collagen and chitosan	981:1056	The results showed that the properties of the scaffolds based on silk fibroin, collagen and chitosan can be modified by chemical cross-linking with DAS and DAC.
33806219	0	38	theme	Physicochemical	50:64	arg1	Properties					66:75	Physicochemical Properties	50:75	Physicochemical Properties of Biopolymeric Materials	50:101	Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials?
33806219	0	39	theme	Good	25:28	arg1	Chitosan					14:21	Dialdehyde Chitosan	3:21	Dialdehyde Chitosan	3:21	Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials?
33806219	0	39	theme	Good	25:28	arg1	Substance					30:38	a Good Substance	23:38	a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials	23:101	Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials?
33806219	7	40	theme	lower	1509:1513	arg1	properties					1526:1535	lower mechanical properties	1509:1535	lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%)	1509:1689	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	3	41	theme	density	640:646	arg1	observations					648:659	density observations	640:659	density observations	640:659	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	1	42	theme	dialdehyde	297:306	arg1	DAC					318:320	DAC	318:320	DAC	318:320	The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC).
33806219	1	42	theme	dialdehyde	297:306	arg1	chitosan					308:315	dialdehyde chitosan	297:315	dialdehyde chitosan (DAC)	297:321	The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC).
33806219	7	43	theme	cross-linked	1312:1323	arg1	materials					1325:1333	DAS cross-linked materials	1308:1333	DAS cross-linked materials	1308:1333	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	4	44	theme	live/dead	816:824	arg1	staining					839:846	live/dead fluorescence staining	816:846	live/dead fluorescence staining	816:846	Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells.
33806219	10	45	used	used	2066:2069	arg2	scaffolds					2007:2015	The scaffolds	2003:2015	The scaffolds presented in the present work	2003:2045	The scaffolds presented in the present work can be potentially used in bone tissue engineering to facilitate healing of small bone defects.
33806219	3	46	theme	electron	671:678	arg1	SEM					700:702	SEM	700:702	SEM	700:702	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	3	46	theme	electron	671:678	arg1	imaging					691:697	scanning electron microscopy imaging	662:697	scanning electron microscopy imaging (SEM)	662:703	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	7	47	contain	had	1381:1383	arg1	they					1376:1379	they	1376:1379	they	1376:1379	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	7	47	contain	had	1381:1383	arg2	density					1391:1397	lower density	1385:1397	lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3)	1385:1503	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	7	47	contain	had	1381:1383	arg2	properties					1526:1535	lower mechanical properties	1509:1535	lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%)	1509:1689	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	3	48	theme	transfer	493:500	arg1	spectroscopy					511:522	Fourier transfer infrared spectroscopy	485:522	Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations	485:659	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	4	49	theme	obtained	904:911	arg1	materials					913:921	the obtained materials	900:921	the obtained materials	900:921	Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells.
33806219	7	50	dep	materials	1708:1716	arg1	comparison					1694:1703	comparison	1694:1703	comparison	1694:1703	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	3	51	with	spectroscopy	511:522	arg1	observations					648:659	density observations	640:659	density observations	640:659	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	3	51	with	spectroscopy	511:522	arg1	measurements					613:624	attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements	529:624	attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements	529:624	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	3	51	with	spectroscopy	511:522	arg1	porosity					627:634	porosity	627:634	porosity	627:634	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	3	52	theme	reflectance	546:556	arg1	FTIR-ATR					566:573	FTIR-ATR	566:573	FTIR-ATR	566:573	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	3	52	theme	reflectance	546:556	arg1	device					558:563	total reflectance device	540:563	total reflectance device (FTIR-ATR)	540:574	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	7	53	theme	2.4	1453:1455	arg1	±					1451:1451	±	1451:1451	±	1451:1451	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	6	54	theme	different	1153:1161	arg1	influence					1163:1171	different influence	1153:1171	different influence	1153:1171	It was found that DAS and DAC have different influence on the properties of biopolymeric scaffolds.
33806219	3	55	theme	attenuated	529:538	arg1	measurements					613:624	attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements	529:624	attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements	529:624	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	7	56	theme	21.8	1446:1449	arg1	±					1451:1451	±	1451:1451	±	1451:1451	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	10	57	theme	bone	2074:2077	arg1	engineering					2086:2096	bone tissue engineering	2074:2096	bone tissue engineering	2074:2096	The scaffolds presented in the present work can be potentially used in bone tissue engineering to facilitate healing of small bone defects.
33806219	7	58	dep	cross-linked	1464:1475	arg1	scaffold					1414:1421	Coll/CTS/30SF scaffold	1400:1421	Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3	1400:1461	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	7	58	dep	cross-linked	1464:1475	arg1	g/cm3					1498:1502	14.6 ± 0.7 g/cm3	1487:1502	14.6 ± 0.7 g/cm3	1487:1502	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	3	59	theme	properties	717:726	arg1	evaluation					728:737	mechanical properties evaluation	706:737	mechanical properties evaluation	706:737	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	3	60	with	imaging	691:697	arg1	observations					648:659	density observations	640:659	density observations	640:659	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	3	60	with	imaging	691:697	arg1	measurements					613:624	attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements	529:624	attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements	529:624	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	3	60	with	imaging	691:697	arg1	porosity					627:634	porosity	627:634	porosity	627:634	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	1	61	theme	dimensional	176:186	arg1	scaffolds					188:196	three dimensional scaffolds	170:196	three dimensional scaffolds based on silk fibroin	170:218	The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC).
33806219	7	62	from	%	1688:1688	arg1	range					1649:1653	the range	1645:1653	the range of 12.67 ±	1645:1664	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	7	63	theme	DAS	1608:1610	arg1	scaffolds					1625:1633	DAS cross-linked scaffolds	1608:1633	DAS cross-linked scaffolds	1608:1633	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	4	64	with	activity	781:788	arg1	staining					839:846	live/dead fluorescence staining	816:846	live/dead fluorescence staining	816:846	Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells.
33806219	4	64	with	activity	781:788	arg1	assay					806:810	AlamarBlue assay	795:810	AlamarBlue assay	795:810	Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells.
33806219	1	65	theme	silk	207:210	arg1	fibroin					212:218	silk fibroin	207:218	silk fibroin	207:218	The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC).
33806219	6	66	contain	have	1148:1151	arg2	influence					1163:1171	different influence	1153:1171	different influence	1153:1171	It was found that DAS and DAC have different influence on the properties of biopolymeric scaffolds.
33806219	6	66	contain	have	1148:1151	arg1	DAS					1136:1138	DAS	1136:1138	DAS	1136:1138	It was found that DAS and DAC have different influence on the properties of biopolymeric scaffolds.
33806219	6	66	contain	have	1148:1151	arg1	DAC					1144:1146	DAC	1144:1146	DAC	1144:1146	It was found that DAS and DAC have different influence on the properties of biopolymeric scaffolds.
33806219	7	67	theme	Coll/CTS/30SF	1400:1412	arg1	scaffold					1414:1421	Coll/CTS/30SF scaffold	1400:1421	Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3	1400:1461	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	9	68	theme	scaffolds	1911:1919	arg1	types					1902:1906	both types	1897:1906	both types of scaffolds	1897:1919	However, the obtained results showed that both types of scaffolds can provide the support required in regenerative medicine and tissue engineering.
33806219	3	69	theme	swelling	577:584	arg1	behavior					586:593	swelling behavior	577:593	swelling behavior	577:593	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	4	70	theme	osteoblast-like	934:948	arg1	cells					950:954	MG-63 osteoblast-like cells	928:954	MG-63 osteoblast-like cells	928:954	Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells.
33806219	10	71	theme	bone	2129:2132	arg1	defects					2134:2140	small bone defects	2123:2140	small bone defects	2123:2140	The scaffolds presented in the present work can be potentially used in bone tissue engineering to facilitate healing of small bone defects.
33806219	1	72	theme	work	120:123	arg1	aim					108:110	The aim	104:110	The aim of this work	104:123	The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC).
33806219	7	73	link	cross-linked	1312:1323	arg1	materials					1325:1333	DAS cross-linked materials	1308:1333	DAS cross-linked materials	1308:1333	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	3	74	theme	content	605:611	arg1	measurements					613:624	attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements	529:624	attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements	529:624	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	7	75	dep	scaffold	1414:1421	arg1	g/cm3					1457:1461	21.8 ± 2.4 g/cm3	1446:1461	Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3	1400:1461	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	7	76	theme	cross-linked	1566:1577	arg1	scaffolds					1579:1587	DAC cross-linked scaffolds	1562:1587	DAC cross-linked scaffolds	1562:1587	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	0	77	theme	Biopolymeric	80:91	arg1	Materials					93:101	Biopolymeric Materials	80:101	Biopolymeric Materials	80:101	Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials?
33806219	1	78	theme	physicochemical	140:154	arg1	blends					243:248	collagen and chitosan blends	221:248	collagen and chitosan blends	221:248	The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC).
33806219	1	78	theme	physicochemical	140:154	arg1	properties					156:165	physicochemical properties	140:165	physicochemical properties of three dimensional scaffolds based on silk fibroin	140:218	The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC).
33806219	7	79	theme	±	1682:1682	arg1	%					1688:1688	19.83 ± 1.30%	1676:1688	19.83 ± 1.30%	1676:1688	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	7	80	dep	%	1302:1302	arg1	%					1341:1341	~2500%	1336:1341	~2500% for DAC cross-linked materials	1336:1372	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	7	81	theme	maximum	1538:1544	arg1	deformation					1546:1556	maximum deformation	1538:1556	maximum deformation for DAC cross-linked scaffolds	1538:1587	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	9	82	theme	tissue	1983:1988	arg1	engineering					1990:2000	tissue engineering	1983:2000	tissue engineering	1983:2000	However, the obtained results showed that both types of scaffolds can provide the support required in regenerative medicine and tissue engineering.
33806219	5	83	theme	silk	1022:1025	arg1	chitosan					1049:1056	chitosan	1049:1056	chitosan	1049:1056	The results showed that the properties of the scaffolds based on silk fibroin, collagen and chitosan can be modified by chemical cross-linking with DAS and DAC.
33806219	5	83	theme	silk	1022:1025	arg1	collagen					1036:1043	collagen	1036:1043	collagen	1036:1043	The results showed that the properties of the scaffolds based on silk fibroin, collagen and chitosan can be modified by chemical cross-linking with DAS and DAC.
33806219	5	83	theme	silk	1022:1025	arg1	fibroin					1027:1033	silk fibroin	1022:1033	silk fibroin	1022:1033	The results showed that the properties of the scaffolds based on silk fibroin, collagen and chitosan can be modified by chemical cross-linking with DAS and DAC.
33806219	7	84	with	cross-linked	1464:1475	arg1	DAC					1482:1484	DAC	1482:1484	DAC	1482:1484	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	7	85	theme	cross-linked	1351:1362	arg1	materials					1364:1372	DAC cross-linked materials	1347:1372	DAC cross-linked materials	1347:1372	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	7	86	link	cross-linked	1566:1577	arg1	scaffolds					1579:1587	DAC cross-linked scaffolds	1562:1587	DAC cross-linked scaffolds	1562:1587	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	4	87	theme	AlamarBlue	795:804	arg1	assay					806:810	AlamarBlue assay	795:810	AlamarBlue assay	795:810	Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells.
33806219	3	88	theme	physicochemical	416:430	arg1	properties					432:441	physicochemical properties	416:441	physicochemical properties	416:441	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	0	89	theme	Dialdehyde	3:12	arg1	Chitosan					14:21	Dialdehyde Chitosan	3:21	Dialdehyde Chitosan	3:21	Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials?
33806219	0	89	theme	Dialdehyde	3:12	arg1	Substance					30:38	a Good Substance	23:38	a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials	23:101	Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials?
33806219	3	90	with	analysis	761:768	arg1	observations					648:659	density observations	640:659	density observations	640:659	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	3	90	with	analysis	761:768	arg1	measurements					613:624	attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements	529:624	attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements	529:624	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	3	90	with	analysis	761:768	arg1	porosity					627:634	porosity	627:634	porosity	627:634	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	8	91	theme	higher	1797:1802	arg1	biocompatibility					1804:1819	higher biocompatibility	1797:1819	higher biocompatibility	1797:1819	Additionally, scaffolds cross-linked with DAS exhibited higher biocompatibility than those cross-linked with DAC.
33806219	7	92	dep	characterized	1255:1267	arg1	%					1302:1302	~4000%	1297:1302	~4000% for DAS cross-linked materials	1297:1333	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	3	93	theme	materials	450:458	arg1	Structure					402:410	Structure	402:410	Structure	402:410	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	3	93	theme	materials	450:458	arg1	properties					432:441	physicochemical properties	416:441	physicochemical properties	416:441	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	7	94	dep	properties	1526:1535	arg1	%					1601:1601	about 69%	1593:1601	about 69%	1593:1601	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	7	95	theme	mechanical	1515:1524	arg1	properties					1526:1535	lower mechanical properties	1509:1535	lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%)	1509:1689	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	0	96	dep	Substance	30:38	arg1	Modify					43:48	Modify	43:48	to Modify Physicochemical Properties of Biopolymeric Materials	40:101	Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials?
33806219	7	97	theme	DAS	1308:1310	arg1	materials					1325:1333	DAS cross-linked materials	1308:1333	DAS cross-linked materials	1308:1333	Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC.
33806219	3	98	theme	microscopy	680:689	arg1	SEM					700:702	SEM	700:702	SEM	700:702	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	3	98	theme	microscopy	680:689	arg1	imaging					691:697	scanning electron microscopy imaging	662:697	scanning electron microscopy imaging (SEM)	662:703	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	3	99	theme	Fourier	485:491	arg1	spectroscopy					511:522	Fourier transfer infrared spectroscopy	485:522	Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations	485:659	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	5	100	mod	modified	1065:1072	arg1	properties					985:994	the properties	981:994	the properties of the scaffolds based on silk fibroin, collagen and chitosan	981:1056	The results showed that the properties of the scaffolds based on silk fibroin, collagen and chitosan can be modified by chemical cross-linking with DAS and DAC.
33806219	5	100	mod	modified	1065:1072	arg3	cross-linking					1086:1098	chemical cross-linking	1077:1098	chemical cross-linking with DAS and DAC	1077:1115	The results showed that the properties of the scaffolds based on silk fibroin, collagen and chitosan can be modified by chemical cross-linking with DAS and DAC.
33806219	10	101	theme	present	2034:2040	arg1	work					2042:2045	the present work	2030:2045	the present work	2030:2045	The scaffolds presented in the present work can be potentially used in bone tissue engineering to facilitate healing of small bone defects.
33806219	3	102	theme	scanning	662:669	arg1	SEM					700:702	SEM	700:702	SEM	700:702	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	3	102	theme	scanning	662:669	arg1	imaging					691:697	scanning electron microscopy imaging	662:697	scanning electron microscopy imaging (SEM)	662:703	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	3	103	with	evaluation	728:737	arg1	observations					648:659	density observations	640:659	density observations	640:659	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	3	103	with	evaluation	728:737	arg1	measurements					613:624	attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements	529:624	attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements	529:624	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	3	103	with	evaluation	728:737	arg1	porosity					627:634	porosity	627:634	porosity	627:634	Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis.
33806219	1	104	theme	collagen	221:228	arg1	blends					243:248	collagen and chitosan blends	221:248	collagen and chitosan blends	221:248	The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC).
33806219	1	104	theme	collagen	221:228	arg1	properties					156:165	physicochemical properties	140:165	physicochemical properties of three dimensional scaffolds based on silk fibroin	140:218	The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC).
33806219	4	105	theme	fluorescence	826:837	arg1	staining					839:846	live/dead fluorescence staining	816:846	live/dead fluorescence staining	816:846	Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells.
33806219	1	106	theme	chitosan	234:241	arg1	blends					243:248	collagen and chitosan blends	221:248	collagen and chitosan blends	221:248	The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC).
33806219	1	106	theme	chitosan	234:241	arg1	properties					156:165	physicochemical properties	140:165	physicochemical properties of three dimensional scaffolds based on silk fibroin	140:218	The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC).
33475461	8	0	theme	bacteria	1175:1182	arg1	majority					1149:1156	the majority	1145:1156	the majority of Gram-negative bacteria, including opportunistic pathogens,	1145:1218	Bacterial cell sorting followed by 16S rRNA sequencing revealed that the majority of Gram-negative bacteria, including opportunistic pathogens, were IgA-coated after the intervention.
33475461	5	1	theme	oral	675:678	arg1	mg					695:696	oral vancomycin 500 mg	675:696	oral vancomycin 500 mg four times per day for 7 days	675:726	Healthy humans and humans with metabolic syndrome received oral vancomycin 500 mg four times per day for 7 days.
33475461	4	2	theme	microbiome	592:601	arg1	composition					603:613	gut microbiome composition	588:613	gut microbiome composition	588:613	Here, we determined the intestinal and systemic Ig response to a major intervention in gut microbiome composition.
33475461	8	3	theme	Gram-negative	1161:1173	arg1	bacteria					1175:1182	Gram-negative bacteria	1161:1182	Gram-negative bacteria	1161:1182	Bacterial cell sorting followed by 16S rRNA sequencing revealed that the majority of Gram-negative bacteria, including opportunistic pathogens, were IgA-coated after the intervention.
33475461	8	3	theme	Gram-negative	1161:1173	arg1	pathogens					1209:1217	opportunistic pathogens	1195:1217	opportunistic pathogens	1195:1217	Bacterial cell sorting followed by 16S rRNA sequencing revealed that the majority of Gram-negative bacteria, including opportunistic pathogens, were IgA-coated after the intervention.
33475461	5	4	theme	vancomycin	680:689	arg1	mg					695:696	oral vancomycin 500 mg	675:696	oral vancomycin 500 mg four times per day for 7 days	675:726	Healthy humans and humans with metabolic syndrome received oral vancomycin 500 mg four times per day for 7 days.
33475461	5	5	theme	Healthy	616:622	arg1	humans					624:629	Healthy humans	616:629	Healthy humans	616:629	Healthy humans and humans with metabolic syndrome received oral vancomycin 500 mg four times per day for 7 days.
33475461	1	6	theme	bacterial	176:184	arg1	components					186:195	bacterial components	176:195	bacterial components	176:195	Intestinal immunoglobulins (Ig) are abundantly secreted antibodies that bind bacteria and bacterial components in the gut.
33475461	4	7	theme	gut	588:590	arg1	composition					603:613	gut microbiome composition	588:613	gut microbiome composition	588:613	Here, we determined the intestinal and systemic Ig response to a major intervention in gut microbiome composition.
33475461	4	8	theme	intestinal	525:534	arg1	response					552:559	the intestinal and systemic Ig response	521:559	the intestinal and systemic Ig response to a major intervention in gut microbiome composition	521:613	Here, we determined the intestinal and systemic Ig response to a major intervention in gut microbiome composition.
33475461	6	9	theme	bacterial	834:842	arg1	flagellin					884:892	flagellin	884:892	flagellin	884:892	Coinciding with a vancomycin-induced increase in Gram-negative bacteria, fecal levels of the immunogenic bacterial components lipopolysaccharide (LPS) and flagellin drastically increased.
33475461	6	9	theme	bacterial	834:842	arg1	lipopolysaccharide					855:872	lipopolysaccharide	855:872	lipopolysaccharide (LPS)	855:878	Coinciding with a vancomycin-induced increase in Gram-negative bacteria, fecal levels of the immunogenic bacterial components lipopolysaccharide (LPS) and flagellin drastically increased.
33475461	6	9	theme	bacterial	834:842	arg1	components					844:853	the immunogenic bacterial components	818:853	the immunogenic bacterial components lipopolysaccharide (LPS) and flagellin	818:892	Coinciding with a vancomycin-induced increase in Gram-negative bacteria, fecal levels of the immunogenic bacterial components lipopolysaccharide (LPS) and flagellin drastically increased.
33475461	7	10	dep	antibodies	928:937	arg1	antibodies					928:937	Intestinal antibodies	917:937	Intestinal antibodies (IgA and IgM)	917:951	Intestinal antibodies (IgA and IgM) significantly increased, whereas peripheral antibodies (IgG, IgA, and IgM) were mostly unaffected by vancomycin treatment.
33475461	7	10	dep	antibodies	928:937	arg1	IgM					948:950	IgM	948:950	IgM	948:950	Intestinal antibodies (IgA and IgM) significantly increased, whereas peripheral antibodies (IgG, IgA, and IgM) were mostly unaffected by vancomycin treatment.
33475461	7	10	dep	antibodies	928:937	arg1	IgA					940:942	IgA	940:942	IgA	940:942	Intestinal antibodies (IgA and IgM) significantly increased, whereas peripheral antibodies (IgG, IgA, and IgM) were mostly unaffected by vancomycin treatment.
33475461	7	11	theme	Intestinal	917:926	arg1	antibodies					928:937	Intestinal antibodies	917:937	Intestinal antibodies (IgA and IgM)	917:951	Intestinal antibodies (IgA and IgM) significantly increased, whereas peripheral antibodies (IgG, IgA, and IgM) were mostly unaffected by vancomycin treatment.
33475461	7	11	theme	Intestinal	917:926	arg1	IgM					948:950	IgM	948:950	IgM	948:950	Intestinal antibodies (IgA and IgM) significantly increased, whereas peripheral antibodies (IgG, IgA, and IgM) were mostly unaffected by vancomycin treatment.
33475461	7	11	theme	Intestinal	917:926	arg1	IgA					940:942	IgA	940:942	IgA	940:942	Intestinal antibodies (IgA and IgM) significantly increased, whereas peripheral antibodies (IgG, IgA, and IgM) were mostly unaffected by vancomycin treatment.
33475461	3	12	from	alterations	397:407	arg1	composition					427:437	gut microbiome composition	412:437	gut microbiome composition	412:437	Ig secretion is regulated by alterations in gut microbiome composition, an event rarely mapped in an intervention setting in humans.
33475461	3	12	from	alterations	397:407	arg1	event					443:447	an event	440:447	an event rarely mapped in an intervention setting in humans	440:498	Ig secretion is regulated by alterations in gut microbiome composition, an event rarely mapped in an intervention setting in humans.
33475461	6	13	theme	Gram-negative	778:790	arg1	bacteria					792:799	Gram-negative bacteria	778:799	Gram-negative bacteria	778:799	Coinciding with a vancomycin-induced increase in Gram-negative bacteria, fecal levels of the immunogenic bacterial components lipopolysaccharide (LPS) and flagellin drastically increased.
33475461	6	14	theme	immunogenic	822:832	arg1	flagellin					884:892	flagellin	884:892	flagellin	884:892	Coinciding with a vancomycin-induced increase in Gram-negative bacteria, fecal levels of the immunogenic bacterial components lipopolysaccharide (LPS) and flagellin drastically increased.
33475461	6	14	theme	immunogenic	822:832	arg1	lipopolysaccharide					855:872	lipopolysaccharide	855:872	lipopolysaccharide (LPS)	855:878	Coinciding with a vancomycin-induced increase in Gram-negative bacteria, fecal levels of the immunogenic bacterial components lipopolysaccharide (LPS) and flagellin drastically increased.
33475461	6	14	theme	immunogenic	822:832	arg1	components					844:853	the immunogenic bacterial components	818:853	the immunogenic bacterial components lipopolysaccharide (LPS) and flagellin	818:892	Coinciding with a vancomycin-induced increase in Gram-negative bacteria, fecal levels of the immunogenic bacterial components lipopolysaccharide (LPS) and flagellin drastically increased.
33475461	0	15	from	treatment	65:73	arg1	humans					78:83	humans	78:83	humans	78:83	Compensatory intestinal immunoglobulin response after vancomycin treatment in humans.
33475461	8	16	theme	Bacterial	1076:1084	arg1	sorting					1091:1097	Bacterial cell sorting	1076:1097	Bacterial cell sorting followed by 16S rRNA sequencing	1076:1129	Bacterial cell sorting followed by 16S rRNA sequencing revealed that the majority of Gram-negative bacteria, including opportunistic pathogens, were IgA-coated after the intervention.
33475461	1	17	from	bacteria	163:170	arg1	gut					204:206	the gut	200:206	the gut	200:206	Intestinal immunoglobulins (Ig) are abundantly secreted antibodies that bind bacteria and bacterial components in the gut.
33475461	1	18	theme	Intestinal	86:95	arg1	Ig					114:115	Ig	114:115	Ig	114:115	Intestinal immunoglobulins (Ig) are abundantly secreted antibodies that bind bacteria and bacterial components in the gut.
33475461	1	18	theme	Intestinal	86:95	arg1	immunoglobulins					97:111	Intestinal immunoglobulins	86:111	Intestinal immunoglobulins (Ig)	86:116	Intestinal immunoglobulins (Ig) are abundantly secreted antibodies that bind bacteria and bacterial components in the gut.
33475461	8	19	theme	opportunistic	1195:1207	arg1	pathogens					1209:1217	opportunistic pathogens	1195:1217	opportunistic pathogens	1195:1217	Bacterial cell sorting followed by 16S rRNA sequencing revealed that the majority of Gram-negative bacteria, including opportunistic pathogens, were IgA-coated after the intervention.
33475461	5	20	theme	metabolic	647:655	arg1	syndrome					657:664	metabolic syndrome	647:664	metabolic syndrome	647:664	Healthy humans and humans with metabolic syndrome received oral vancomycin 500 mg four times per day for 7 days.
33475461	3	21	theme	microbiome	416:425	arg1	composition					427:437	gut microbiome composition	412:437	gut microbiome composition	412:437	Ig secretion is regulated by alterations in gut microbiome composition, an event rarely mapped in an intervention setting in humans.
33475461	3	21	theme	microbiome	416:425	arg1	event					443:447	an event	440:447	an event rarely mapped in an intervention setting in humans	440:498	Ig secretion is regulated by alterations in gut microbiome composition, an event rarely mapped in an intervention setting in humans.
33475461	0	22	theme	intestinal	13:22	arg1	response					39:46	Compensatory intestinal immunoglobulin response	0:46	Compensatory intestinal immunoglobulin response	0:46	Compensatory intestinal immunoglobulin response after vancomycin treatment in humans.
33475461	9	23	theme	bacterial	1370:1378	arg1	components					1380:1389	bacterial components	1370:1389	bacterial components	1370:1389	We suggest that the intestinal Ig response after vancomycin treatment prevents the intrusion of pathogens and bacterial components into systemic sites.
33475461	9	24	theme	components	1380:1389	arg1	intrusion					1343:1351	the intrusion	1339:1351	the intrusion of pathogens and bacterial components into systemic sites	1339:1409	We suggest that the intestinal Ig response after vancomycin treatment prevents the intrusion of pathogens and bacterial components into systemic sites.
33475461	2	25	theme	intestinal	343:352	arg1	cells					361:365	intestinal immune cells	343:365	intestinal immune cells	343:365	This binding is considered to accelerate bacterial transit time and prevent the interaction of potentially immunogenic compounds with intestinal immune cells.
33475461	9	26	theme	pathogens	1356:1364	arg1	intrusion					1343:1351	the intrusion	1339:1351	the intrusion of pathogens and bacterial components into systemic sites	1339:1409	We suggest that the intestinal Ig response after vancomycin treatment prevents the intrusion of pathogens and bacterial components into systemic sites.
33475461	0	27	theme	Compensatory	0:11	arg1	response					39:46	Compensatory intestinal immunoglobulin response	0:46	Compensatory intestinal immunoglobulin response	0:46	Compensatory intestinal immunoglobulin response after vancomycin treatment in humans.
33475461	4	28	theme	major	566:570	arg1	intervention					572:583	a major intervention	564:583	a major intervention in gut microbiome composition	564:613	Here, we determined the intestinal and systemic Ig response to a major intervention in gut microbiome composition.
33475461	8	29	theme	cell	1086:1089	arg1	sorting					1091:1097	Bacterial cell sorting	1076:1097	Bacterial cell sorting followed by 16S rRNA sequencing	1076:1129	Bacterial cell sorting followed by 16S rRNA sequencing revealed that the majority of Gram-negative bacteria, including opportunistic pathogens, were IgA-coated after the intervention.
33475461	6	30	theme	fecal	802:806	arg1	levels					808:813	fecal levels	802:813	fecal levels of the immunogenic bacterial components lipopolysaccharide (LPS) and flagellin	802:892	Coinciding with a vancomycin-induced increase in Gram-negative bacteria, fecal levels of the immunogenic bacterial components lipopolysaccharide (LPS) and flagellin drastically increased.
33475461	4	31	from	intervention	572:583	arg1	composition					603:613	gut microbiome composition	588:613	gut microbiome composition	588:613	Here, we determined the intestinal and systemic Ig response to a major intervention in gut microbiome composition.
33475461	2	32	theme	compounds	328:336	arg1	interaction					289:299	the interaction	285:299	the interaction of potentially immunogenic compounds with intestinal immune cells	285:365	This binding is considered to accelerate bacterial transit time and prevent the interaction of potentially immunogenic compounds with intestinal immune cells.
33475461	6	33	theme	components	844:853	arg1	levels					808:813	fecal levels	802:813	fecal levels of the immunogenic bacterial components lipopolysaccharide (LPS) and flagellin	802:892	Coinciding with a vancomycin-induced increase in Gram-negative bacteria, fecal levels of the immunogenic bacterial components lipopolysaccharide (LPS) and flagellin drastically increased.
33475461	0	34	theme	immunoglobulin	24:37	arg1	response					39:46	Compensatory intestinal immunoglobulin response	0:46	Compensatory intestinal immunoglobulin response	0:46	Compensatory intestinal immunoglobulin response after vancomycin treatment in humans.
33475461	9	35	theme	systemic	1396:1403	arg1	sites					1405:1409	systemic sites	1396:1409	systemic sites	1396:1409	We suggest that the intestinal Ig response after vancomycin treatment prevents the intrusion of pathogens and bacterial components into systemic sites.
33475461	2	36	theme	immunogenic	316:326	arg1	compounds					328:336	potentially immunogenic compounds	304:336	potentially immunogenic compounds	304:336	This binding is considered to accelerate bacterial transit time and prevent the interaction of potentially immunogenic compounds with intestinal immune cells.
33475461	6	37	dep	components	844:853	arg1	LPS					875:877	LPS	875:877	LPS	875:877	Coinciding with a vancomycin-induced increase in Gram-negative bacteria, fecal levels of the immunogenic bacterial components lipopolysaccharide (LPS) and flagellin drastically increased.
33475461	6	37	dep	components	844:853	arg1	flagellin					884:892	flagellin	884:892	flagellin	884:892	Coinciding with a vancomycin-induced increase in Gram-negative bacteria, fecal levels of the immunogenic bacterial components lipopolysaccharide (LPS) and flagellin drastically increased.
33475461	6	37	dep	components	844:853	arg1	lipopolysaccharide					855:872	lipopolysaccharide	855:872	lipopolysaccharide (LPS)	855:878	Coinciding with a vancomycin-induced increase in Gram-negative bacteria, fecal levels of the immunogenic bacterial components lipopolysaccharide (LPS) and flagellin drastically increased.
33475461	6	37	dep	components	844:853	arg1	components					844:853	the immunogenic bacterial components	818:853	the immunogenic bacterial components lipopolysaccharide (LPS) and flagellin	818:892	Coinciding with a vancomycin-induced increase in Gram-negative bacteria, fecal levels of the immunogenic bacterial components lipopolysaccharide (LPS) and flagellin drastically increased.
33475461	0	38	theme	vancomycin	54:63	arg1	treatment					65:73	vancomycin treatment	54:73	vancomycin treatment in humans	54:83	Compensatory intestinal immunoglobulin response after vancomycin treatment in humans.
33475461	8	39	theme	rRNA	1115:1118	arg1	sequencing					1120:1129	16S rRNA sequencing	1111:1129	16S rRNA sequencing	1111:1129	Bacterial cell sorting followed by 16S rRNA sequencing revealed that the majority of Gram-negative bacteria, including opportunistic pathogens, were IgA-coated after the intervention.
33475461	2	40	theme	bacterial	250:258	arg1	time					268:271	bacterial transit time	250:271	bacterial transit time	250:271	This binding is considered to accelerate bacterial transit time and prevent the interaction of potentially immunogenic compounds with intestinal immune cells.
33475461	3	41	theme	intervention	469:480	arg1	setting					482:488	an intervention setting	466:488	an intervention setting in humans	466:498	Ig secretion is regulated by alterations in gut microbiome composition, an event rarely mapped in an intervention setting in humans.
33475461	4	42	theme	Ig	549:550	arg1	response					552:559	the intestinal and systemic Ig response	521:559	the intestinal and systemic Ig response to a major intervention in gut microbiome composition	521:613	Here, we determined the intestinal and systemic Ig response to a major intervention in gut microbiome composition.
33475461	8	43	theme	16S	1111:1113	arg1	sequencing					1120:1129	16S rRNA sequencing	1111:1129	16S rRNA sequencing	1111:1129	Bacterial cell sorting followed by 16S rRNA sequencing revealed that the majority of Gram-negative bacteria, including opportunistic pathogens, were IgA-coated after the intervention.
33475461	5	44	dep	mg	695:696	arg1	times					703:707	four times	698:707	oral vancomycin 500 mg four times per day for 7 days	675:726	Healthy humans and humans with metabolic syndrome received oral vancomycin 500 mg four times per day for 7 days.
33475461	4	45	theme	systemic	540:547	arg1	response					552:559	the intestinal and systemic Ig response	521:559	the intestinal and systemic Ig response to a major intervention in gut microbiome composition	521:613	Here, we determined the intestinal and systemic Ig response to a major intervention in gut microbiome composition.
33475461	7	46	theme	vancomycin	1054:1063	arg1	treatment					1065:1073	vancomycin treatment	1054:1073	vancomycin treatment	1054:1073	Intestinal antibodies (IgA and IgM) significantly increased, whereas peripheral antibodies (IgG, IgA, and IgM) were mostly unaffected by vancomycin treatment.
33475461	9	47	theme	intestinal	1280:1289	arg1	response					1294:1301	the intestinal Ig response	1276:1301	the intestinal Ig response after vancomycin treatment	1276:1328	We suggest that the intestinal Ig response after vancomycin treatment prevents the intrusion of pathogens and bacterial components into systemic sites.
33475461	3	48	from	setting	482:488	arg1	humans					493:498	humans	493:498	humans	493:498	Ig secretion is regulated by alterations in gut microbiome composition, an event rarely mapped in an intervention setting in humans.
33475461	7	49	theme	peripheral	986:995	arg1	antibodies					997:1006	peripheral antibodies	986:1006	peripheral antibodies (IgG, IgA, and IgM)	986:1026	Intestinal antibodies (IgA and IgM) significantly increased, whereas peripheral antibodies (IgG, IgA, and IgM) were mostly unaffected by vancomycin treatment.
33475461	7	49	theme	peripheral	986:995	arg1	IgA					1014:1016	IgA	1014:1016	IgA	1014:1016	Intestinal antibodies (IgA and IgM) significantly increased, whereas peripheral antibodies (IgG, IgA, and IgM) were mostly unaffected by vancomycin treatment.
33475461	7	49	theme	peripheral	986:995	arg1	IgM					1023:1025	IgM	1023:1025	IgM	1023:1025	Intestinal antibodies (IgA and IgM) significantly increased, whereas peripheral antibodies (IgG, IgA, and IgM) were mostly unaffected by vancomycin treatment.
33475461	7	49	theme	peripheral	986:995	arg1	IgG					1009:1011	IgG	1009:1011	IgG	1009:1011	Intestinal antibodies (IgA and IgM) significantly increased, whereas peripheral antibodies (IgG, IgA, and IgM) were mostly unaffected by vancomycin treatment.
33475461	9	50	theme	Ig	1291:1292	arg1	response					1294:1301	the intestinal Ig response	1276:1301	the intestinal Ig response after vancomycin treatment	1276:1328	We suggest that the intestinal Ig response after vancomycin treatment prevents the intrusion of pathogens and bacterial components into systemic sites.
33475461	6	51	from	increase	766:773	arg1	bacteria					792:799	Gram-negative bacteria	778:799	Gram-negative bacteria	778:799	Coinciding with a vancomycin-induced increase in Gram-negative bacteria, fecal levels of the immunogenic bacterial components lipopolysaccharide (LPS) and flagellin drastically increased.
33475461	5	52	with	humans	624:629	arg1	syndrome					657:664	metabolic syndrome	647:664	metabolic syndrome	647:664	Healthy humans and humans with metabolic syndrome received oral vancomycin 500 mg four times per day for 7 days.
33475461	0	53	dep	response	39:46	arg1	treatment					65:73	vancomycin treatment	54:73	vancomycin treatment in humans	54:83	Compensatory intestinal immunoglobulin response after vancomycin treatment in humans.
33475461	7	54	dep	antibodies	997:1006	arg1	antibodies					997:1006	peripheral antibodies	986:1006	peripheral antibodies (IgG, IgA, and IgM)	986:1026	Intestinal antibodies (IgA and IgM) significantly increased, whereas peripheral antibodies (IgG, IgA, and IgM) were mostly unaffected by vancomycin treatment.
33475461	7	54	dep	antibodies	997:1006	arg1	IgA					1014:1016	IgA	1014:1016	IgA	1014:1016	Intestinal antibodies (IgA and IgM) significantly increased, whereas peripheral antibodies (IgG, IgA, and IgM) were mostly unaffected by vancomycin treatment.
33475461	7	54	dep	antibodies	997:1006	arg1	IgM					1023:1025	IgM	1023:1025	IgM	1023:1025	Intestinal antibodies (IgA and IgM) significantly increased, whereas peripheral antibodies (IgG, IgA, and IgM) were mostly unaffected by vancomycin treatment.
33475461	7	54	dep	antibodies	997:1006	arg1	IgG					1009:1011	IgG	1009:1011	IgG	1009:1011	Intestinal antibodies (IgA and IgM) significantly increased, whereas peripheral antibodies (IgG, IgA, and IgM) were mostly unaffected by vancomycin treatment.
33475461	2	55	with	interaction	289:299	arg1	cells					361:365	intestinal immune cells	343:365	intestinal immune cells	343:365	This binding is considered to accelerate bacterial transit time and prevent the interaction of potentially immunogenic compounds with intestinal immune cells.
33475461	6	56	theme	vancomycin-induced	747:764	arg1	increase					766:773	a vancomycin-induced increase	745:773	a vancomycin-induced increase in Gram-negative bacteria	745:799	Coinciding with a vancomycin-induced increase in Gram-negative bacteria, fecal levels of the immunogenic bacterial components lipopolysaccharide (LPS) and flagellin drastically increased.
33475461	2	57	theme	immune	354:359	arg1	cells					361:365	intestinal immune cells	343:365	intestinal immune cells	343:365	This binding is considered to accelerate bacterial transit time and prevent the interaction of potentially immunogenic compounds with intestinal immune cells.
33475461	9	58	theme	vancomycin	1309:1318	arg1	treatment					1320:1328	vancomycin treatment	1309:1328	vancomycin treatment	1309:1328	We suggest that the intestinal Ig response after vancomycin treatment prevents the intrusion of pathogens and bacterial components into systemic sites.
33475461	3	59	theme	Ig	368:369	arg1	secretion					371:379	Ig secretion	368:379	Ig secretion	368:379	Ig secretion is regulated by alterations in gut microbiome composition, an event rarely mapped in an intervention setting in humans.
33475461	2	60	theme	transit	260:266	arg1	time					268:271	bacterial transit time	250:271	bacterial transit time	250:271	This binding is considered to accelerate bacterial transit time and prevent the interaction of potentially immunogenic compounds with intestinal immune cells.
33475461	1	61	from	components	186:195	arg1	gut					204:206	the gut	200:206	the gut	200:206	Intestinal immunoglobulins (Ig) are abundantly secreted antibodies that bind bacteria and bacterial components in the gut.
33475461	3	62	theme	gut	412:414	arg1	composition					427:437	gut microbiome composition	412:437	gut microbiome composition	412:437	Ig secretion is regulated by alterations in gut microbiome composition, an event rarely mapped in an intervention setting in humans.
33475461	3	62	theme	gut	412:414	arg1	event					443:447	an event	440:447	an event rarely mapped in an intervention setting in humans	440:498	Ig secretion is regulated by alterations in gut microbiome composition, an event rarely mapped in an intervention setting in humans.
33475461	5	63	with	humans	635:640	arg1	syndrome					657:664	metabolic syndrome	647:664	metabolic syndrome	647:664	Healthy humans and humans with metabolic syndrome received oral vancomycin 500 mg four times per day for 7 days.
32820296	0	0	theme	wound	96:100	arg1	healing					102:108	dermal wound healing	89:108	dermal wound healing	89:108	Bioresponsive supramolecular hydrogels for hemostasis, infection control and accelerated dermal wound healing.
32820296	7	1	theme	AG/PVCL-TA	1230:1239	arg1	scaffold					1241:1248	The AG/PVCL-TA scaffold	1226:1248	The AG/PVCL-TA scaffold	1226:1248	The AG/PVCL-TA scaffold was capable of releasing a therapeutic dose of TA in a sustained manner under physiological temperature-pH conditions.
32820296	7	1	theme	AG/PVCL-TA	1230:1239	arg1	capable					1254:1260	capable	1254:1260	capable	1254:1260	The AG/PVCL-TA scaffold was capable of releasing a therapeutic dose of TA in a sustained manner under physiological temperature-pH conditions.
32820296	9	2	theme	rapid	1712:1716	arg1	healing					1724:1730	rapid wound healing	1712:1730	rapid wound healing	1712:1730	The wound healing performance of AG/PVCL-TA was further confirmed in skin excision wound models, which demonstrated the potential application of AG/PVCL-TA for skin regeneration and rapid wound healing.
32820296	9	3	theme	wound	1718:1722	arg1	healing					1724:1730	rapid wound healing	1712:1730	rapid wound healing	1712:1730	The wound healing performance of AG/PVCL-TA was further confirmed in skin excision wound models, which demonstrated the potential application of AG/PVCL-TA for skin regeneration and rapid wound healing.
32820296	2	4	theme	temperature-pH	398:411	arg1	scaffold					459:466	a biodegradable, temperature-pH dual responsive supramolecular hydrogel (SHG) scaffold	381:466	scaffold	459:466	Herein, we demonstrate the fabrication of a biodegradable, temperature-pH dual responsive supramolecular hydrogel (SHG) scaffold based on sodium alginate/poly(N-vinyl caprolactam) (AG/PVCL) through free radical polymerization and the subsequent chemical and ionic cross-linking.
32820296	6	5	theme	SA	1195:1196	arg1	composition					1180:1190	the composition	1176:1190	the composition of SA, VCL, TA and cross-linkers	1176:1223	The SHG formation was successfully balanced by varying the composition of SA, VCL, TA and cross-linkers.
32820296	0	6	theme	dermal	89:94	arg1	healing					102:108	dermal wound healing	89:108	dermal wound healing	89:108	Bioresponsive supramolecular hydrogels for hemostasis, infection control and accelerated dermal wound healing.
32820296	9	7	theme	wound	1534:1538	arg1	healing					1540:1546	The wound healing	1530:1546	The wound healing performance of AG/PVCL-TA	1530:1572	The wound healing performance of AG/PVCL-TA was further confirmed in skin excision wound models, which demonstrated the potential application of AG/PVCL-TA for skin regeneration and rapid wound healing.
32820296	5	8	theme	structure	922:930	arg1	abilities					952:960	the polyphenol-arm structure and diverse bonding abilities	903:960	the polyphenol-arm structure and diverse bonding abilities of TA	903:966	Notably, the polyphenol-arm structure and diverse bonding abilities of TA can hold polymer chains through multiple bonding and co-ordinate cross-linking, which were vital in the formation of the mechanically robust AG/PVCL-TA.
32820296	8	9	theme	fibroblast	1508:1517	arg1	line					1524:1527	the 3T3 fibroblast cell line	1500:1527	the 3T3 fibroblast cell line	1500:1527	AG/PVCL-TA displayed excellent free radical scavenging, anti-inflammatory, anti-bacterial, and cell proliferation activity towards the 3T3 fibroblast cell line.
32820296	9	10	theme	healing	1540:1546	arg1	performance					1548:1558	The wound healing performance	1530:1558	The wound healing performance of AG/PVCL-TA	1530:1572	The wound healing performance of AG/PVCL-TA was further confirmed in skin excision wound models, which demonstrated the potential application of AG/PVCL-TA for skin regeneration and rapid wound healing.
32820296	1	11	theme	ideal	301:305	arg1	materials					307:315	ideal materials	301:315	ideal materials	301:315	Injectable, drug-releasing hydrogel scaffolds with multifunctional properties including hemostasis and anti-bacterial activity are essential for successful wound healing; however, designing ideal materials is still challenging.
32820296	6	12	theme	SHG	1125:1127	arg1	formation					1129:1137	The SHG formation	1121:1137	The SHG formation	1121:1137	The SHG formation was successfully balanced by varying the composition of SA, VCL, TA and cross-linkers.
32820296	5	13	theme	co-ordinate	1021:1031	arg1	cross-linking					1033:1045	co-ordinate cross-linking	1021:1045	co-ordinate cross-linking	1021:1045	Notably, the polyphenol-arm structure and diverse bonding abilities of TA can hold polymer chains through multiple bonding and co-ordinate cross-linking, which were vital in the formation of the mechanically robust AG/PVCL-TA.
32820296	5	14	theme	polyphenol-arm	907:920	arg1	structure					922:930	polyphenol-arm structure	907:930	polyphenol-arm structure	907:930	Notably, the polyphenol-arm structure and diverse bonding abilities of TA can hold polymer chains through multiple bonding and co-ordinate cross-linking, which were vital in the formation of the mechanically robust AG/PVCL-TA.
32820296	9	15	theme	wound	1613:1617	arg1	models					1619:1624	skin excision wound models	1599:1624	skin excision wound models	1599:1624	The wound healing performance of AG/PVCL-TA was further confirmed in skin excision wound models, which demonstrated the potential application of AG/PVCL-TA for skin regeneration and rapid wound healing.
32820296	5	16	theme	TA	965:966	arg1	abilities					952:960	the polyphenol-arm structure and diverse bonding abilities	903:960	the polyphenol-arm structure and diverse bonding abilities of TA	903:966	Notably, the polyphenol-arm structure and diverse bonding abilities of TA can hold polymer chains through multiple bonding and co-ordinate cross-linking, which were vital in the formation of the mechanically robust AG/PVCL-TA.
32820296	9	17	theme	excision	1604:1611	arg1	wound					1613:1617	skin excision wound	1599:1617	skin excision wound models	1599:1624	The wound healing performance of AG/PVCL-TA was further confirmed in skin excision wound models, which demonstrated the potential application of AG/PVCL-TA for skin regeneration and rapid wound healing.
32820296	4	18	theme	AG/PVCL-TA	787:796	arg1	system					798:803	the AG/PVCL-TA system	783:803	the AG/PVCL-TA system	783:803	In the AG/PVCL-TA system, TA acts as a therapeutic molecule and also substitutes as an effective gelation binder.
32820296	9	19	theme	AG/PVCL-TA	1563:1572	arg1	performance					1548:1558	The wound healing performance	1530:1558	The wound healing performance of AG/PVCL-TA	1530:1572	The wound healing performance of AG/PVCL-TA was further confirmed in skin excision wound models, which demonstrated the potential application of AG/PVCL-TA for skin regeneration and rapid wound healing.
32820296	5	20	theme	diverse	936:942	arg1	bonding					944:950	diverse bonding	936:950	diverse bonding	936:950	Notably, the polyphenol-arm structure and diverse bonding abilities of TA can hold polymer chains through multiple bonding and co-ordinate cross-linking, which were vital in the formation of the mechanically robust AG/PVCL-TA.
32820296	1	21	with	scaffolds	147:155	arg1	activity					229:236	anti-bacterial activity	214:236	anti-bacterial activity	214:236	Injectable, drug-releasing hydrogel scaffolds with multifunctional properties including hemostasis and anti-bacterial activity are essential for successful wound healing; however, designing ideal materials is still challenging.
32820296	1	21	with	scaffolds	147:155	arg1	properties					178:187	multifunctional properties	162:187	multifunctional properties including hemostasis and anti-bacterial activity	162:236	Injectable, drug-releasing hydrogel scaffolds with multifunctional properties including hemostasis and anti-bacterial activity are essential for successful wound healing; however, designing ideal materials is still challenging.
32820296	1	21	with	scaffolds	147:155	arg1	hemostasis					199:208	hemostasis	199:208	hemostasis	199:208	Injectable, drug-releasing hydrogel scaffolds with multifunctional properties including hemostasis and anti-bacterial activity are essential for successful wound healing; however, designing ideal materials is still challenging.
32820296	5	22	theme	multiple	1000:1007	arg1	bonding					1009:1015	multiple bonding	1000:1015	multiple bonding	1000:1015	Notably, the polyphenol-arm structure and diverse bonding abilities of TA can hold polymer chains through multiple bonding and co-ordinate cross-linking, which were vital in the formation of the mechanically robust AG/PVCL-TA.
32820296	7	23	theme	physiological	1328:1340	arg1	conditions					1357:1366	physiological temperature-pH conditions	1328:1366	physiological temperature-pH conditions	1328:1366	The AG/PVCL-TA scaffold was capable of releasing a therapeutic dose of TA in a sustained manner under physiological temperature-pH conditions.
32820296	2	24	theme	SHG	454:456	arg1	scaffold					459:466	a biodegradable, temperature-pH dual responsive supramolecular hydrogel (SHG) scaffold	381:466	scaffold	459:466	Herein, we demonstrate the fabrication of a biodegradable, temperature-pH dual responsive supramolecular hydrogel (SHG) scaffold based on sodium alginate/poly(N-vinyl caprolactam) (AG/PVCL) through free radical polymerization and the subsequent chemical and ionic cross-linking.
32820296	3	25	theme	wound	741:745	arg1	healing					747:753	wound healing	741:753	wound healing	741:753	A natural therapeutic molecule, tannic acid (TA)-incorporated SHG (AG/PVCL-TA), was also fabricated and its hemostatic and wound healing efficiency were studied.
32820296	8	26	theme	excellent	1390:1398	arg1	activity					1483:1490	excellent free radical scavenging, anti-inflammatory, anti-bacterial, and cell proliferation activity	1390:1490	excellent free radical scavenging, anti-inflammatory, anti-bacterial, and cell proliferation activity towards the 3T3 fibroblast cell line	1390:1527	AG/PVCL-TA displayed excellent free radical scavenging, anti-inflammatory, anti-bacterial, and cell proliferation activity towards the 3T3 fibroblast cell line.
32820296	1	27	theme	anti-bacterial	214:227	arg1	activity					229:236	anti-bacterial activity	214:236	anti-bacterial activity	214:236	Injectable, drug-releasing hydrogel scaffolds with multifunctional properties including hemostasis and anti-bacterial activity are essential for successful wound healing; however, designing ideal materials is still challenging.
32820296	0	28	theme	supramolecular	14:27	arg1	hydrogels					29:37	Bioresponsive supramolecular hydrogels	0:37	Bioresponsive supramolecular hydrogels for hemostasis, infection	0:63	Bioresponsive supramolecular hydrogels for hemostasis, infection control and accelerated dermal wound healing.
32820296	5	29	from	formation	1072:1080	arg1	vital					1059:1063	vital	1059:1063	vital	1059:1063	Notably, the polyphenol-arm structure and diverse bonding abilities of TA can hold polymer chains through multiple bonding and co-ordinate cross-linking, which were vital in the formation of the mechanically robust AG/PVCL-TA.
32820296	4	30	theme	gelation	877:884	arg1	binder					886:891	an effective gelation binder	864:891	an effective gelation binder	864:891	In the AG/PVCL-TA system, TA acts as a therapeutic molecule and also substitutes as an effective gelation binder.
32820296	3	31	theme	natural	620:626	arg1	molecule					640:647	A natural therapeutic molecule	618:647	A natural therapeutic molecule	618:647	A natural therapeutic molecule, tannic acid (TA)-incorporated SHG (AG/PVCL-TA), was also fabricated and its hemostatic and wound healing efficiency were studied.
32820296	3	31	theme	natural	620:626	arg1	SHG					680:682	tannic acid (TA)-incorporated SHG	650:682	tannic acid (TA)-incorporated SHG (AG/PVCL-TA)	650:695	A natural therapeutic molecule, tannic acid (TA)-incorporated SHG (AG/PVCL-TA), was also fabricated and its hemostatic and wound healing efficiency were studied.
32820296	2	32	theme	hydrogel	444:451	arg1	scaffold					459:466	a biodegradable, temperature-pH dual responsive supramolecular hydrogel (SHG) scaffold	381:466	scaffold	459:466	Herein, we demonstrate the fabrication of a biodegradable, temperature-pH dual responsive supramolecular hydrogel (SHG) scaffold based on sodium alginate/poly(N-vinyl caprolactam) (AG/PVCL) through free radical polymerization and the subsequent chemical and ionic cross-linking.
32820296	0	33	theme	Bioresponsive	0:12	arg1	hydrogels					29:37	Bioresponsive supramolecular hydrogels	0:37	Bioresponsive supramolecular hydrogels for hemostasis, infection	0:63	Bioresponsive supramolecular hydrogels for hemostasis, infection control and accelerated dermal wound healing.
32820296	4	34	theme	effective	867:875	arg1	binder					886:891	an effective gelation binder	864:891	an effective gelation binder	864:891	In the AG/PVCL-TA system, TA acts as a therapeutic molecule and also substitutes as an effective gelation binder.
32820296	3	35	theme	therapeutic	628:638	arg1	molecule					640:647	A natural therapeutic molecule	618:647	A natural therapeutic molecule	618:647	A natural therapeutic molecule, tannic acid (TA)-incorporated SHG (AG/PVCL-TA), was also fabricated and its hemostatic and wound healing efficiency were studied.
32820296	3	35	theme	therapeutic	628:638	arg1	SHG					680:682	tannic acid (TA)-incorporated SHG	650:682	tannic acid (TA)-incorporated SHG (AG/PVCL-TA)	650:695	A natural therapeutic molecule, tannic acid (TA)-incorporated SHG (AG/PVCL-TA), was also fabricated and its hemostatic and wound healing efficiency were studied.
32820296	5	36	from	vital	1059:1063	arg1	formation					1072:1080	the formation	1068:1080	the formation of the mechanically robust AG/PVCL-TA	1068:1118	Notably, the polyphenol-arm structure and diverse bonding abilities of TA can hold polymer chains through multiple bonding and co-ordinate cross-linking, which were vital in the formation of the mechanically robust AG/PVCL-TA.
32820296	2	37	theme	supramolecular	429:442	arg1	scaffold					459:466	a biodegradable, temperature-pH dual responsive supramolecular hydrogel (SHG) scaffold	381:466	scaffold	459:466	Herein, we demonstrate the fabrication of a biodegradable, temperature-pH dual responsive supramolecular hydrogel (SHG) scaffold based on sodium alginate/poly(N-vinyl caprolactam) (AG/PVCL) through free radical polymerization and the subsequent chemical and ionic cross-linking.
32820296	8	38	theme	cell	1519:1522	arg1	line					1524:1527	the 3T3 fibroblast cell line	1500:1527	the 3T3 fibroblast cell line	1500:1527	AG/PVCL-TA displayed excellent free radical scavenging, anti-inflammatory, anti-bacterial, and cell proliferation activity towards the 3T3 fibroblast cell line.
32820296	6	39	theme	cross-linkers	1211:1223	arg1	composition					1180:1190	the composition	1176:1190	the composition of SA, VCL, TA and cross-linkers	1176:1223	The SHG formation was successfully balanced by varying the composition of SA, VCL, TA and cross-linkers.
32820296	1	40	theme	Injectable	111:120	arg1	scaffolds					147:155	Injectable, drug-releasing hydrogel scaffolds	111:155	Injectable, drug-releasing hydrogel scaffolds with multifunctional properties including hemostasis and anti-bacterial activity	111:236	Injectable, drug-releasing hydrogel scaffolds with multifunctional properties including hemostasis and anti-bacterial activity are essential for successful wound healing; however, designing ideal materials is still challenging.
32820296	8	41	theme	anti-bacterial	1444:1457	arg1	activity					1483:1490	excellent free radical scavenging, anti-inflammatory, anti-bacterial, and cell proliferation activity	1390:1490	excellent free radical scavenging, anti-inflammatory, anti-bacterial, and cell proliferation activity towards the 3T3 fibroblast cell line	1390:1527	AG/PVCL-TA displayed excellent free radical scavenging, anti-inflammatory, anti-bacterial, and cell proliferation activity towards the 3T3 fibroblast cell line.
32820296	2	42	theme	responsive	418:427	arg1	scaffold					459:466	a biodegradable, temperature-pH dual responsive supramolecular hydrogel (SHG) scaffold	381:466	scaffold	459:466	Herein, we demonstrate the fabrication of a biodegradable, temperature-pH dual responsive supramolecular hydrogel (SHG) scaffold based on sodium alginate/poly(N-vinyl caprolactam) (AG/PVCL) through free radical polymerization and the subsequent chemical and ionic cross-linking.
32820296	2	43	theme	sodium	477:482	arg1	alginate/poly					484:496	sodium alginate/poly	477:496	sodium alginate/poly(N-vinyl caprolactam) (AG/PVCL) through free radical polymerization and the subsequent chemical and ionic cross-linking	477:615	Herein, we demonstrate the fabrication of a biodegradable, temperature-pH dual responsive supramolecular hydrogel (SHG) scaffold based on sodium alginate/poly(N-vinyl caprolactam) (AG/PVCL) through free radical polymerization and the subsequent chemical and ionic cross-linking.
32820296	2	43	theme	sodium	477:482	arg1	caprolactam					506:516	N-vinyl caprolactam	498:516	N-vinyl caprolactam	498:516	Herein, we demonstrate the fabrication of a biodegradable, temperature-pH dual responsive supramolecular hydrogel (SHG) scaffold based on sodium alginate/poly(N-vinyl caprolactam) (AG/PVCL) through free radical polymerization and the subsequent chemical and ionic cross-linking.
32820296	2	43	theme	sodium	477:482	arg1	AG/PVCL					520:526	AG/PVCL	520:526	AG/PVCL	520:526	Herein, we demonstrate the fabrication of a biodegradable, temperature-pH dual responsive supramolecular hydrogel (SHG) scaffold based on sodium alginate/poly(N-vinyl caprolactam) (AG/PVCL) through free radical polymerization and the subsequent chemical and ionic cross-linking.
32820296	3	44	dep	hemostatic	726:735	arg1	efficiency					755:764	efficiency	755:764	efficiency	755:764	A natural therapeutic molecule, tannic acid (TA)-incorporated SHG (AG/PVCL-TA), was also fabricated and its hemostatic and wound healing efficiency were studied.
32820296	2	45	theme	ionic	597:601	arg1	cross-linking					603:615	the subsequent chemical and ionic cross-linking	569:615	cross-linking	603:615	Herein, we demonstrate the fabrication of a biodegradable, temperature-pH dual responsive supramolecular hydrogel (SHG) scaffold based on sodium alginate/poly(N-vinyl caprolactam) (AG/PVCL) through free radical polymerization and the subsequent chemical and ionic cross-linking.
32820296	6	46	theme	TA	1204:1205	arg1	composition					1180:1190	the composition	1176:1190	the composition of SA, VCL, TA and cross-linkers	1176:1223	The SHG formation was successfully balanced by varying the composition of SA, VCL, TA and cross-linkers.
32820296	5	47	theme	polymer	977:983	arg1	chains					985:990	polymer chains	977:990	polymer chains	977:990	Notably, the polyphenol-arm structure and diverse bonding abilities of TA can hold polymer chains through multiple bonding and co-ordinate cross-linking, which were vital in the formation of the mechanically robust AG/PVCL-TA.
32820296	1	48	theme	multifunctional	162:176	arg1	activity					229:236	anti-bacterial activity	214:236	anti-bacterial activity	214:236	Injectable, drug-releasing hydrogel scaffolds with multifunctional properties including hemostasis and anti-bacterial activity are essential for successful wound healing; however, designing ideal materials is still challenging.
32820296	1	48	theme	multifunctional	162:176	arg1	properties					178:187	multifunctional properties	162:187	multifunctional properties including hemostasis and anti-bacterial activity	162:236	Injectable, drug-releasing hydrogel scaffolds with multifunctional properties including hemostasis and anti-bacterial activity are essential for successful wound healing; however, designing ideal materials is still challenging.
32820296	1	48	theme	multifunctional	162:176	arg1	hemostasis					199:208	hemostasis	199:208	hemostasis	199:208	Injectable, drug-releasing hydrogel scaffolds with multifunctional properties including hemostasis and anti-bacterial activity are essential for successful wound healing; however, designing ideal materials is still challenging.
32820296	5	49	theme	bonding	944:950	arg1	abilities					952:960	the polyphenol-arm structure and diverse bonding abilities	903:960	the polyphenol-arm structure and diverse bonding abilities of TA	903:966	Notably, the polyphenol-arm structure and diverse bonding abilities of TA can hold polymer chains through multiple bonding and co-ordinate cross-linking, which were vital in the formation of the mechanically robust AG/PVCL-TA.
32820296	1	50	theme	successful	256:265	arg1	healing					273:279	successful wound healing	256:279	successful wound healing	256:279	Injectable, drug-releasing hydrogel scaffolds with multifunctional properties including hemostasis and anti-bacterial activity are essential for successful wound healing; however, designing ideal materials is still challenging.
32820296	3	51	theme	tannic	650:655	arg1	TA					663:664	TA	663:664	TA	663:664	A natural therapeutic molecule, tannic acid (TA)-incorporated SHG (AG/PVCL-TA), was also fabricated and its hemostatic and wound healing efficiency were studied.
32820296	3	51	theme	tannic	650:655	arg1	acid					657:660	tannic acid	650:660	tannic acid (TA)-incorporated SHG (AG/PVCL-TA)	650:695	A natural therapeutic molecule, tannic acid (TA)-incorporated SHG (AG/PVCL-TA), was also fabricated and its hemostatic and wound healing efficiency were studied.
32820296	7	52	theme	temperature-pH	1342:1355	arg1	conditions					1357:1366	physiological temperature-pH conditions	1328:1366	physiological temperature-pH conditions	1328:1366	The AG/PVCL-TA scaffold was capable of releasing a therapeutic dose of TA in a sustained manner under physiological temperature-pH conditions.
32820296	2	53	theme	scaffold	459:466	arg1	fabrication					366:376	the fabrication	362:376	the fabrication of a biodegradable, temperature-pH dual responsive supramolecular hydrogel (SHG) scaffold based on sodium alginate/poly(N-vinyl caprolactam) (AG/PVCL) through free radical polymerization and the subsequent chemical and ionic cross-linking	362:615	Herein, we demonstrate the fabrication of a biodegradable, temperature-pH dual responsive supramolecular hydrogel (SHG) scaffold based on sodium alginate/poly(N-vinyl caprolactam) (AG/PVCL) through free radical polymerization and the subsequent chemical and ionic cross-linking.
32820296	9	54	theme	skin	1690:1693	arg1	regeneration					1695:1706	skin regeneration	1690:1706	skin regeneration	1690:1706	The wound healing performance of AG/PVCL-TA was further confirmed in skin excision wound models, which demonstrated the potential application of AG/PVCL-TA for skin regeneration and rapid wound healing.
32820296	5	55	theme	AG/PVCL-TA	1109:1118	arg1	formation					1072:1080	the formation	1068:1080	the formation of the mechanically robust AG/PVCL-TA	1068:1118	Notably, the polyphenol-arm structure and diverse bonding abilities of TA can hold polymer chains through multiple bonding and co-ordinate cross-linking, which were vital in the formation of the mechanically robust AG/PVCL-TA.
32820296	3	56	theme	acid	657:660	arg1	AG/PVCL-TA					685:694	AG/PVCL-TA	685:694	AG/PVCL-TA	685:694	A natural therapeutic molecule, tannic acid (TA)-incorporated SHG (AG/PVCL-TA), was also fabricated and its hemostatic and wound healing efficiency were studied.
32820296	3	56	theme	acid	657:660	arg1	SHG					680:682	tannic acid (TA)-incorporated SHG	650:682	tannic acid (TA)-incorporated SHG (AG/PVCL-TA)	650:695	A natural therapeutic molecule, tannic acid (TA)-incorporated SHG (AG/PVCL-TA), was also fabricated and its hemostatic and wound healing efficiency were studied.
32820296	3	56	theme	acid	657:660	arg1	molecule					640:647	A natural therapeutic molecule	618:647	A natural therapeutic molecule	618:647	A natural therapeutic molecule, tannic acid (TA)-incorporated SHG (AG/PVCL-TA), was also fabricated and its hemostatic and wound healing efficiency were studied.
32820296	1	57	theme	wound	267:271	arg1	healing					273:279	successful wound healing	256:279	successful wound healing	256:279	Injectable, drug-releasing hydrogel scaffolds with multifunctional properties including hemostasis and anti-bacterial activity are essential for successful wound healing; however, designing ideal materials is still challenging.
32820296	8	58	theme	scavenging	1413:1422	arg1	activity					1483:1490	excellent free radical scavenging, anti-inflammatory, anti-bacterial, and cell proliferation activity	1390:1490	excellent free radical scavenging, anti-inflammatory, anti-bacterial, and cell proliferation activity towards the 3T3 fibroblast cell line	1390:1527	AG/PVCL-TA displayed excellent free radical scavenging, anti-inflammatory, anti-bacterial, and cell proliferation activity towards the 3T3 fibroblast cell line.
32820296	8	59	theme	cell	1464:1467	arg1	proliferation					1469:1481	cell proliferation	1464:1481	cell proliferation	1464:1481	AG/PVCL-TA displayed excellent free radical scavenging, anti-inflammatory, anti-bacterial, and cell proliferation activity towards the 3T3 fibroblast cell line.
32820296	8	60	theme	radical	1405:1411	arg1	scavenging					1413:1422	free radical scavenging	1400:1422	free radical scavenging	1400:1422	AG/PVCL-TA displayed excellent free radical scavenging, anti-inflammatory, anti-bacterial, and cell proliferation activity towards the 3T3 fibroblast cell line.
32820296	2	61	theme	biodegradable	383:395	arg1	scaffold					459:466	a biodegradable, temperature-pH dual responsive supramolecular hydrogel (SHG) scaffold	381:466	scaffold	459:466	Herein, we demonstrate the fabrication of a biodegradable, temperature-pH dual responsive supramolecular hydrogel (SHG) scaffold based on sodium alginate/poly(N-vinyl caprolactam) (AG/PVCL) through free radical polymerization and the subsequent chemical and ionic cross-linking.
32820296	9	62	theme	skin	1599:1602	arg1	wound					1613:1617	skin excision wound	1599:1617	skin excision wound models	1599:1624	The wound healing performance of AG/PVCL-TA was further confirmed in skin excision wound models, which demonstrated the potential application of AG/PVCL-TA for skin regeneration and rapid wound healing.
32820296	9	63	theme	AG/PVCL-TA	1675:1684	arg1	application					1660:1670	the potential application	1646:1670	the potential application of AG/PVCL-TA for skin regeneration and rapid wound healing	1646:1730	The wound healing performance of AG/PVCL-TA was further confirmed in skin excision wound models, which demonstrated the potential application of AG/PVCL-TA for skin regeneration and rapid wound healing.
32820296	7	64	theme	TA	1297:1298	arg1	dose					1289:1292	a therapeutic dose	1275:1292	a therapeutic dose of TA	1275:1298	The AG/PVCL-TA scaffold was capable of releasing a therapeutic dose of TA in a sustained manner under physiological temperature-pH conditions.
32820296	1	65	theme	hydrogel	138:145	arg1	scaffolds					147:155	Injectable, drug-releasing hydrogel scaffolds	111:155	Injectable, drug-releasing hydrogel scaffolds with multifunctional properties including hemostasis and anti-bacterial activity	111:236	Injectable, drug-releasing hydrogel scaffolds with multifunctional properties including hemostasis and anti-bacterial activity are essential for successful wound healing; however, designing ideal materials is still challenging.
32820296	7	66	theme	therapeutic	1277:1287	arg1	dose					1289:1292	a therapeutic dose	1275:1292	a therapeutic dose of TA	1275:1298	The AG/PVCL-TA scaffold was capable of releasing a therapeutic dose of TA in a sustained manner under physiological temperature-pH conditions.
32820296	8	67	theme	proliferation	1469:1481	arg1	activity					1483:1490	excellent free radical scavenging, anti-inflammatory, anti-bacterial, and cell proliferation activity	1390:1490	excellent free radical scavenging, anti-inflammatory, anti-bacterial, and cell proliferation activity towards the 3T3 fibroblast cell line	1390:1527	AG/PVCL-TA displayed excellent free radical scavenging, anti-inflammatory, anti-bacterial, and cell proliferation activity towards the 3T3 fibroblast cell line.
32820296	8	68	theme	anti-inflammatory	1425:1441	arg1	activity					1483:1490	excellent free radical scavenging, anti-inflammatory, anti-bacterial, and cell proliferation activity	1390:1490	excellent free radical scavenging, anti-inflammatory, anti-bacterial, and cell proliferation activity towards the 3T3 fibroblast cell line	1390:1527	AG/PVCL-TA displayed excellent free radical scavenging, anti-inflammatory, anti-bacterial, and cell proliferation activity towards the 3T3 fibroblast cell line.
32820296	8	69	theme	free	1400:1403	arg1	scavenging					1413:1422	free radical scavenging	1400:1422	free radical scavenging	1400:1422	AG/PVCL-TA displayed excellent free radical scavenging, anti-inflammatory, anti-bacterial, and cell proliferation activity towards the 3T3 fibroblast cell line.
32820296	8	70	theme	3T3	1504:1506	arg1	line					1524:1527	the 3T3 fibroblast cell line	1500:1527	the 3T3 fibroblast cell line	1500:1527	AG/PVCL-TA displayed excellent free radical scavenging, anti-inflammatory, anti-bacterial, and cell proliferation activity towards the 3T3 fibroblast cell line.
32820296	3	71	theme	-incorporated	666:678	arg1	AG/PVCL-TA					685:694	AG/PVCL-TA	685:694	AG/PVCL-TA	685:694	A natural therapeutic molecule, tannic acid (TA)-incorporated SHG (AG/PVCL-TA), was also fabricated and its hemostatic and wound healing efficiency were studied.
32820296	3	71	theme	-incorporated	666:678	arg1	SHG					680:682	tannic acid (TA)-incorporated SHG	650:682	tannic acid (TA)-incorporated SHG (AG/PVCL-TA)	650:695	A natural therapeutic molecule, tannic acid (TA)-incorporated SHG (AG/PVCL-TA), was also fabricated and its hemostatic and wound healing efficiency were studied.
32820296	3	71	theme	-incorporated	666:678	arg1	molecule					640:647	A natural therapeutic molecule	618:647	A natural therapeutic molecule	618:647	A natural therapeutic molecule, tannic acid (TA)-incorporated SHG (AG/PVCL-TA), was also fabricated and its hemostatic and wound healing efficiency were studied.
32820296	5	72	theme	robust	1102:1107	arg1	AG/PVCL-TA					1109:1118	the mechanically robust AG/PVCL-TA	1085:1118	the mechanically robust AG/PVCL-TA	1085:1118	Notably, the polyphenol-arm structure and diverse bonding abilities of TA can hold polymer chains through multiple bonding and co-ordinate cross-linking, which were vital in the formation of the mechanically robust AG/PVCL-TA.
32820296	2	73	theme	dual	413:416	arg1	scaffold					459:466	a biodegradable, temperature-pH dual responsive supramolecular hydrogel (SHG) scaffold	381:466	scaffold	459:466	Herein, we demonstrate the fabrication of a biodegradable, temperature-pH dual responsive supramolecular hydrogel (SHG) scaffold based on sodium alginate/poly(N-vinyl caprolactam) (AG/PVCL) through free radical polymerization and the subsequent chemical and ionic cross-linking.
32820296	2	74	theme	radical	542:548	arg1	polymerization					550:563	free radical polymerization	537:563	free radical polymerization	537:563	Herein, we demonstrate the fabrication of a biodegradable, temperature-pH dual responsive supramolecular hydrogel (SHG) scaffold based on sodium alginate/poly(N-vinyl caprolactam) (AG/PVCL) through free radical polymerization and the subsequent chemical and ionic cross-linking.
32820296	7	75	theme	sustained	1305:1313	arg1	manner					1315:1320	a sustained manner	1303:1320	a sustained manner	1303:1320	The AG/PVCL-TA scaffold was capable of releasing a therapeutic dose of TA in a sustained manner under physiological temperature-pH conditions.
32820296	4	76	theme	therapeutic	819:829	arg1	molecule					831:838	a therapeutic molecule	817:838	a therapeutic molecule	817:838	In the AG/PVCL-TA system, TA acts as a therapeutic molecule and also substitutes as an effective gelation binder.
32820296	4	76	theme	therapeutic	819:829	arg1	TA					806:807	TA	806:807	TA	806:807	In the AG/PVCL-TA system, TA acts as a therapeutic molecule and also substitutes as an effective gelation binder.
32820296	1	77	dep	Injectable	111:120	arg1	drug-releasing					123:136	drug-releasing	123:136	drug-releasing	123:136	Injectable, drug-releasing hydrogel scaffolds with multifunctional properties including hemostasis and anti-bacterial activity are essential for successful wound healing; however, designing ideal materials is still challenging.
32820296	6	78	theme	VCL	1199:1201	arg1	composition					1180:1190	the composition	1176:1190	the composition of SA, VCL, TA and cross-linkers	1176:1223	The SHG formation was successfully balanced by varying the composition of SA, VCL, TA and cross-linkers.
32820296	9	79	theme	potential	1650:1658	arg1	application					1660:1670	the potential application	1646:1670	the potential application of AG/PVCL-TA for skin regeneration and rapid wound healing	1646:1730	The wound healing performance of AG/PVCL-TA was further confirmed in skin excision wound models, which demonstrated the potential application of AG/PVCL-TA for skin regeneration and rapid wound healing.
32820296	2	80	theme	free	537:540	arg1	polymerization					550:563	free radical polymerization	537:563	free radical polymerization	537:563	Herein, we demonstrate the fabrication of a biodegradable, temperature-pH dual responsive supramolecular hydrogel (SHG) scaffold based on sodium alginate/poly(N-vinyl caprolactam) (AG/PVCL) through free radical polymerization and the subsequent chemical and ionic cross-linking.
32820296	2	81	theme	N-vinyl	498:504	arg1	alginate/poly					484:496	sodium alginate/poly	477:496	sodium alginate/poly(N-vinyl caprolactam) (AG/PVCL) through free radical polymerization and the subsequent chemical and ionic cross-linking	477:615	Herein, we demonstrate the fabrication of a biodegradable, temperature-pH dual responsive supramolecular hydrogel (SHG) scaffold based on sodium alginate/poly(N-vinyl caprolactam) (AG/PVCL) through free radical polymerization and the subsequent chemical and ionic cross-linking.
32820296	2	81	theme	N-vinyl	498:504	arg1	caprolactam					506:516	N-vinyl caprolactam	498:516	N-vinyl caprolactam	498:516	Herein, we demonstrate the fabrication of a biodegradable, temperature-pH dual responsive supramolecular hydrogel (SHG) scaffold based on sodium alginate/poly(N-vinyl caprolactam) (AG/PVCL) through free radical polymerization and the subsequent chemical and ionic cross-linking.
33748287	2	0	with	mice	400:403	arg1	colitis					418:424	chronic colitis	410:424	chronic colitis	410:424	This study was aimed at investigating the effect of NAM on the gut microbiota and short-chain fatty acids (SCFAs) in mice with chronic colitis.
33748287	3	1	theme	sodium	510:515	arg1	administration					471:484	administration	471:484	administration of 1.5% dextran sulfate sodium (DSS)	471:521	Colitis was induced in C57BL/6 male mice by administration of 1.5% dextran sulfate sodium (DSS), and the mice were intraperitoneally injected with normal saline (NS) or NAM.
33748287	1	2	theme	anti-inflammatory	255:271	arg1	activity					273:280	anti-inflammatory activity	255:280	anti-inflammatory activity	255:280	Vitamin B (nicotinamide (NAM)), one of the most important nutritional components for humans, exerts anti-inflammatory activity.
33748287	3	3	theme	dextran	494:500	arg1	DSS					518:520	DSS	518:520	DSS	518:520	Colitis was induced in C57BL/6 male mice by administration of 1.5% dextran sulfate sodium (DSS), and the mice were intraperitoneally injected with normal saline (NS) or NAM.
33748287	3	3	theme	dextran	494:500	arg1	sodium					510:515	dextran sulfate sodium	494:515	1.5% dextran sulfate sodium (DSS)	489:521	Colitis was induced in C57BL/6 male mice by administration of 1.5% dextran sulfate sodium (DSS), and the mice were intraperitoneally injected with normal saline (NS) or NAM.
33748287	4	4	theme	NAM	601:603	arg1	treatment					605:613	NAM treatment	601:613	NAM treatment	601:613	NAM treatment ameliorated weight loss and changes in colon length, disease activity index (DAI) score, and histologic scores.
33748287	8	5	theme	microbiota	1691:1700	arg1	functions					1670:1678	the functions	1666:1678	the functions of the gut microbiota	1666:1700	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis indicated that NAM treatment restored disruptions in the functions of the gut microbiota (replication and repair, cell motility) in mice with DSS-induced colitis.
33748287	8	6	theme	Phylogenetic	1513:1524	arg1	Investigation					1526:1538	Phylogenetic Investigation	1513:1538	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis	1513:1611	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis indicated that NAM treatment restored disruptions in the functions of the gut microbiota (replication and repair, cell motility) in mice with DSS-induced colitis.
33748287	5	7	theme	immunosorbent	751:763	arg1	assay					765:769	enzyme-linked immunosorbent assay	737:769	enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells	737:799	Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment.
33748287	5	7	theme	immunosorbent	751:763	arg1	ELISA					772:776	ELISA	772:776	ELISA	772:776	Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment.
33748287	10	8	theme	NAM	1904:1906	arg1	treatment					1908:1916	NAM treatment	1904:1916	NAM treatment	1904:1916	Our results suggest that NAM treatment could alleviate DSS-induced chronic colitis in mice by inhibiting inflammation and regulating the composition and function of gut microbiota.
33748287	1	9	theme	most	198:201	arg1	components					225:234	the most important nutritional components	194:234	the most important nutritional components for humans	194:245	Vitamin B (nicotinamide (NAM)), one of the most important nutritional components for humans, exerts anti-inflammatory activity.
33748287	10	10	theme	DSS-induced	1934:1944	arg1	colitis					1954:1960	DSS-induced chronic colitis	1934:1960	DSS-induced chronic colitis in mice	1934:1968	Our results suggest that NAM treatment could alleviate DSS-induced chronic colitis in mice by inhibiting inflammation and regulating the composition and function of gut microbiota.
33748287	4	11	theme	colon	654:658	arg1	length					660:665	colon length	654:665	colon length	654:665	NAM treatment ameliorated weight loss and changes in colon length, disease activity index (DAI) score, and histologic scores.
33748287	4	12	theme	activity	676:683	arg1	DAI					692:694	DAI	692:694	DAI	692:694	NAM treatment ameliorated weight loss and changes in colon length, disease activity index (DAI) score, and histologic scores.
33748287	4	12	theme	activity	676:683	arg1	index					685:689	disease activity index	668:689	disease activity index (DAI) score	668:701	NAM treatment ameliorated weight loss and changes in colon length, disease activity index (DAI) score, and histologic scores.
33748287	8	13	theme	cell	1727:1730	arg1	replication					1703:1713	replication	1703:1713	replication	1703:1713	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis indicated that NAM treatment restored disruptions in the functions of the gut microbiota (replication and repair, cell motility) in mice with DSS-induced colitis.
33748287	8	13	theme	cell	1727:1730	arg1	motility					1732:1739	cell motility	1727:1739	cell motility	1727:1739	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis indicated that NAM treatment restored disruptions in the functions of the gut microbiota (replication and repair, cell motility) in mice with DSS-induced colitis.
33748287	3	14	theme	normal	574:579	arg1	NS					589:590	NS	589:590	NS	589:590	Colitis was induced in C57BL/6 male mice by administration of 1.5% dextran sulfate sodium (DSS), and the mice were intraperitoneally injected with normal saline (NS) or NAM.
33748287	3	14	theme	normal	574:579	arg1	saline					581:586	normal saline	574:586	normal saline (NS)	574:591	Colitis was induced in C57BL/6 male mice by administration of 1.5% dextran sulfate sodium (DSS), and the mice were intraperitoneally injected with normal saline (NS) or NAM.
33748287	5	15	theme	interleukin-	828:839	arg1	level					819:823	the level	815:823	the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A	815:938	Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment.
33748287	7	16	theme	colitis	1498:1504	arg1	group					1506:1510	the NAM+chronic colitis group	1482:1510	the NAM+chronic colitis group	1482:1510	In addition, NAM modulated specific bacteria, including Odoribacter, Flexispira, and Bifidobacterium, in the NAM+chronic colitis group.
33748287	0	17	theme	Dextran	25:31	arg1	Sulfate					33:39	Dextran Sulfate	25:39	Dextran Sulfate Sodium-Induced Chronic Colitis in Mice through Its Anti-Inflammatory Properties	25:119	Nicotinamide Ameliorates Dextran Sulfate Sodium-Induced Chronic Colitis in Mice through Its Anti-Inflammatory Properties and Modulates the Gut Microbiota.
33748287	8	18	theme	States	1587:1592	arg1	analysis					1604:1611	Unobserved States (PICRUSt) analysis	1576:1611	Unobserved States (PICRUSt) analysis	1576:1611	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis indicated that NAM treatment restored disruptions in the functions of the gut microbiota (replication and repair, cell motility) in mice with DSS-induced colitis.
33748287	5	19	theme	cells	795:799	arg1	analysis					779:786	enzyme-linked immunosorbent assay (ELISA) analysis	737:786	enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells	737:799	Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment.
33748287	6	20	theme	content	1161:1167	arg1	sequencing					1126:1135	16S rRNA sequencing	1117:1135	16S rRNA sequencing of the large intestinal content	1117:1167	Then, 16S rRNA sequencing of the large intestinal content was performed, and analysis of alpha diversity and beta diversity showed that the richness of the gut microbiota was decreased in the DSS group compared to the control group and restored after NAM treatment.
33748287	10	21	dep	composition	2016:2026	arg1	the					2012:2014	the	2012:2014	the	2012:2014	Our results suggest that NAM treatment could alleviate DSS-induced chronic colitis in mice by inhibiting inflammation and regulating the composition and function of gut microbiota.
33748287	6	22	theme	large	1144:1148	arg1	content					1161:1167	the large intestinal content	1140:1167	the large intestinal content	1140:1167	Then, 16S rRNA sequencing of the large intestinal content was performed, and analysis of alpha diversity and beta diversity showed that the richness of the gut microbiota was decreased in the DSS group compared to the control group and restored after NAM treatment.
33748287	8	23	theme	analysis	1604:1611	arg1	Reconstruction					1558:1571	Reconstruction	1558:1571	Reconstruction of Unobserved States (PICRUSt) analysis	1558:1611	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis indicated that NAM treatment restored disruptions in the functions of the gut microbiota (replication and repair, cell motility) in mice with DSS-induced colitis.
33748287	5	24	theme	IL-12p70	850:857	arg1	level					819:823	the level	815:823	the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A	815:938	Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment.
33748287	1	25	theme	Vitamin	155:161	arg1	components					225:234	the most important nutritional components	194:234	the most important nutritional components for humans	194:245	Vitamin B (nicotinamide (NAM)), one of the most important nutritional components for humans, exerts anti-inflammatory activity.
33748287	1	25	theme	Vitamin	155:161	arg1	one					187:189	one	187:189	one	187:189	Vitamin B (nicotinamide (NAM)), one of the most important nutritional components for humans, exerts anti-inflammatory activity.
33748287	1	25	theme	Vitamin	155:161	arg1	B					163:163	Vitamin B	155:163	Vitamin B (nicotinamide (NAM))	155:184	Vitamin B (nicotinamide (NAM)), one of the most important nutritional components for humans, exerts anti-inflammatory activity.
33748287	0	26	from	Colitis	64:70	arg1	Mice					75:78	Mice	75:78	Mice	75:78	Nicotinamide Ameliorates Dextran Sulfate Sodium-Induced Chronic Colitis in Mice through Its Anti-Inflammatory Properties and Modulates the Gut Microbiota.
33748287	2	27	dep	microbiota	350:359	arg1	the					342:344	the	342:344	the	342:344	This study was aimed at investigating the effect of NAM on the gut microbiota and short-chain fatty acids (SCFAs) in mice with chronic colitis.
33748287	5	28	theme	control	1025:1031	arg1	group					1033:1037	the control group	1021:1037	the control group	1021:1037	Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment.
33748287	5	29	theme	IL-17A	933:938	arg1	level					819:823	the level	815:823	the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A	815:938	Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment.
33748287	0	30	theme	Chronic	56:62	arg1	Colitis					64:70	Dextran Sulfate Sodium-Induced Chronic Colitis	25:70	Dextran Sulfate Sodium-Induced Chronic Colitis in Mice through Its Anti-Inflammatory Properties	25:119	Nicotinamide Ameliorates Dextran Sulfate Sodium-Induced Chronic Colitis in Mice through Its Anti-Inflammatory Properties and Modulates the Gut Microbiota.
33748287	5	31	theme	IL-1β	860:864	arg1	γ					919:919	IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ	860:919	γ	919:919	Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment.
33748287	2	32	theme	short-chain	365:375	arg1	SCFAs					390:394	SCFAs	390:394	SCFAs	390:394	This study was aimed at investigating the effect of NAM on the gut microbiota and short-chain fatty acids (SCFAs) in mice with chronic colitis.
33748287	2	32	theme	short-chain	365:375	arg1	acids					383:387	short-chain fatty acids	365:387	short-chain fatty acids (SCFAs)	365:395	This study was aimed at investigating the effect of NAM on the gut microbiota and short-chain fatty acids (SCFAs) in mice with chronic colitis.
33748287	6	33	theme	beta	1220:1223	arg1	diversity					1225:1233	beta diversity	1220:1233	beta diversity	1220:1233	Then, 16S rRNA sequencing of the large intestinal content was performed, and analysis of alpha diversity and beta diversity showed that the richness of the gut microbiota was decreased in the DSS group compared to the control group and restored after NAM treatment.
33748287	9	34	theme	valeric	1824:1830	arg1	acid					1832:1835	valeric acid	1824:1835	valeric acid	1824:1835	Furthermore, NAM also restored the reduction in valeric acid in mice with DSS-induced chronic colitis.
33748287	1	35	dep	B	163:163	arg1	NAM					180:182	NAM	180:182	NAM	180:182	Vitamin B (nicotinamide (NAM)), one of the most important nutritional components for humans, exerts anti-inflammatory activity.
33748287	1	35	dep	B	163:163	arg1	nicotinamide					166:177	nicotinamide	166:177	nicotinamide (NAM)	166:183	Vitamin B (nicotinamide (NAM)), one of the most important nutritional components for humans, exerts anti-inflammatory activity.
33748287	6	36	theme	diversity	1206:1214	arg1	analysis					1188:1195	analysis	1188:1195	analysis of alpha diversity and beta diversity	1188:1233	Then, 16S rRNA sequencing of the large intestinal content was performed, and analysis of alpha diversity and beta diversity showed that the richness of the gut microbiota was decreased in the DSS group compared to the control group and restored after NAM treatment.
33748287	5	37	theme	colitis	995:1001	arg1	group					1003:1007	the chronic colitis group	983:1007	the chronic colitis group compared to the control group	983:1037	Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment.
33748287	0	38	theme	Sodium-Induced	41:54	arg1	Colitis					64:70	Dextran Sulfate Sodium-Induced Chronic Colitis	25:70	Dextran Sulfate Sodium-Induced Chronic Colitis in Mice through Its Anti-Inflammatory Properties	25:119	Nicotinamide Ameliorates Dextran Sulfate Sodium-Induced Chronic Colitis in Mice through Its Anti-Inflammatory Properties and Modulates the Gut Microbiota.
33748287	5	39	theme	TNF-	891:894	arg1	γ					919:919	IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ	860:919	γ	919:919	Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment.
33748287	3	40	theme	male	458:461	arg1	mice					463:466	C57BL/6 male mice	450:466	C57BL/6 male mice	450:466	Colitis was induced in C57BL/6 male mice by administration of 1.5% dextran sulfate sodium (DSS), and the mice were intraperitoneally injected with normal saline (NS) or NAM.
33748287	5	41	theme	α	897:897	arg1	γ					919:919	IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ	860:919	γ	919:919	Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment.
33748287	2	42	from	acids	383:387	arg1	mice					400:403	mice	400:403	mice with chronic colitis	400:424	This study was aimed at investigating the effect of NAM on the gut microbiota and short-chain fatty acids (SCFAs) in mice with chronic colitis.
33748287	9	43	theme	chronic	1862:1868	arg1	colitis					1870:1876	DSS-induced chronic colitis	1850:1876	DSS-induced chronic colitis	1850:1876	Furthermore, NAM also restored the reduction in valeric acid in mice with DSS-induced chronic colitis.
33748287	6	44	theme	gut	1267:1269	arg1	microbiota					1271:1280	the gut microbiota	1263:1280	the gut microbiota	1263:1280	Then, 16S rRNA sequencing of the large intestinal content was performed, and analysis of alpha diversity and beta diversity showed that the richness of the gut microbiota was decreased in the DSS group compared to the control group and restored after NAM treatment.
33748287	1	45	theme	important	203:211	arg1	components					225:234	the most important nutritional components	194:234	the most important nutritional components for humans	194:245	Vitamin B (nicotinamide (NAM)), one of the most important nutritional components for humans, exerts anti-inflammatory activity.
33748287	9	46	from	reduction	1811:1819	arg1	mice					1840:1843	mice	1840:1843	mice with DSS-induced chronic colitis	1840:1876	Furthermore, NAM also restored the reduction in valeric acid in mice with DSS-induced chronic colitis.
33748287	9	46	from	reduction	1811:1819	arg1	acid					1832:1835	valeric acid	1824:1835	valeric acid	1824:1835	Furthermore, NAM also restored the reduction in valeric acid in mice with DSS-induced chronic colitis.
33748287	4	47	theme	histologic	708:717	arg1	scores					719:724	histologic scores	708:724	histologic scores	708:724	NAM treatment ameliorated weight loss and changes in colon length, disease activity index (DAI) score, and histologic scores.
33748287	1	48	theme	components	225:234	arg1	components					225:234	the most important nutritional components	194:234	the most important nutritional components for humans	194:245	Vitamin B (nicotinamide (NAM)), one of the most important nutritional components for humans, exerts anti-inflammatory activity.
33748287	1	48	theme	components	225:234	arg1	B					163:163	Vitamin B	155:163	Vitamin B (nicotinamide (NAM))	155:184	Vitamin B (nicotinamide (NAM)), one of the most important nutritional components for humans, exerts anti-inflammatory activity.
33748287	1	48	theme	components	225:234	arg1	one					187:189	one	187:189	one	187:189	Vitamin B (nicotinamide (NAM)), one of the most important nutritional components for humans, exerts anti-inflammatory activity.
33748287	5	49	theme	factor-	882:888	arg1	γ					919:919	IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ	860:919	γ	919:919	Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment.
33748287	5	50	dep	γ	919:919	arg1	interferon-					900:910	IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ	860:919	interferon-	900:910	Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment.
33748287	5	50	dep	γ	919:919	arg1	tumor					867:871	IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ	860:919	tumor	867:871	Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment.
33748287	2	51	from	effect	325:330	arg1	SCFAs					390:394	SCFAs	390:394	SCFAs	390:394	This study was aimed at investigating the effect of NAM on the gut microbiota and short-chain fatty acids (SCFAs) in mice with chronic colitis.
33748287	2	51	from	effect	325:330	arg1	acids					383:387	short-chain fatty acids	365:387	short-chain fatty acids (SCFAs)	365:395	This study was aimed at investigating the effect of NAM on the gut microbiota and short-chain fatty acids (SCFAs) in mice with chronic colitis.
33748287	2	51	from	effect	325:330	arg1	microbiota					350:359	gut microbiota	346:359	gut microbiota	346:359	This study was aimed at investigating the effect of NAM on the gut microbiota and short-chain fatty acids (SCFAs) in mice with chronic colitis.
33748287	8	52	with	mice	1745:1748	arg1	colitis					1767:1773	DSS-induced colitis	1755:1773	DSS-induced colitis	1755:1773	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis indicated that NAM treatment restored disruptions in the functions of the gut microbiota (replication and repair, cell motility) in mice with DSS-induced colitis.
33748287	3	53	theme	sulfate	502:508	arg1	DSS					518:520	DSS	518:520	DSS	518:520	Colitis was induced in C57BL/6 male mice by administration of 1.5% dextran sulfate sodium (DSS), and the mice were intraperitoneally injected with normal saline (NS) or NAM.
33748287	3	53	theme	sulfate	502:508	arg1	sodium					510:515	dextran sulfate sodium	494:515	1.5% dextran sulfate sodium (DSS)	489:521	Colitis was induced in C57BL/6 male mice by administration of 1.5% dextran sulfate sodium (DSS), and the mice were intraperitoneally injected with normal saline (NS) or NAM.
33748287	10	54	theme	microbiota	2048:2057	arg1	function					2032:2039	function	2032:2039	function	2032:2039	Our results suggest that NAM treatment could alleviate DSS-induced chronic colitis in mice by inhibiting inflammation and regulating the composition and function of gut microbiota.
33748287	10	54	theme	microbiota	2048:2057	arg1	composition					2016:2026	composition	2016:2026	composition	2016:2026	Our results suggest that NAM treatment could alleviate DSS-induced chronic colitis in mice by inhibiting inflammation and regulating the composition and function of gut microbiota.
33748287	2	55	theme	NAM	335:337	arg1	effect					325:330	the effect	321:330	the effect of NAM on the gut microbiota and short-chain fatty acids (SCFAs) in mice with chronic colitis	321:424	This study was aimed at investigating the effect of NAM on the gut microbiota and short-chain fatty acids (SCFAs) in mice with chronic colitis.
33748287	3	56	theme	%	492:492	arg1	DSS					518:520	DSS	518:520	DSS	518:520	Colitis was induced in C57BL/6 male mice by administration of 1.5% dextran sulfate sodium (DSS), and the mice were intraperitoneally injected with normal saline (NS) or NAM.
33748287	3	56	theme	%	492:492	arg1	sodium					510:515	dextran sulfate sodium	494:515	1.5% dextran sulfate sodium (DSS)	489:521	Colitis was induced in C57BL/6 male mice by administration of 1.5% dextran sulfate sodium (DSS), and the mice were intraperitoneally injected with normal saline (NS) or NAM.
33748287	8	57	theme	gut	1687:1689	arg1	microbiota					1691:1700	the gut microbiota	1683:1700	the gut microbiota	1683:1700	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis indicated that NAM treatment restored disruptions in the functions of the gut microbiota (replication and repair, cell motility) in mice with DSS-induced colitis.
33748287	6	58	theme	DSS	1303:1305	arg1	group					1307:1311	the DSS group	1299:1311	the DSS group	1299:1311	Then, 16S rRNA sequencing of the large intestinal content was performed, and analysis of alpha diversity and beta diversity showed that the richness of the gut microbiota was decreased in the DSS group compared to the control group and restored after NAM treatment.
33748287	7	59	theme	specific	1404:1411	arg1	Odoribacter					1433:1443	Odoribacter	1433:1443	Odoribacter	1433:1443	In addition, NAM modulated specific bacteria, including Odoribacter, Flexispira, and Bifidobacterium, in the NAM+chronic colitis group.
33748287	7	59	theme	specific	1404:1411	arg1	Bifidobacterium					1462:1476	Bifidobacterium	1462:1476	Bifidobacterium	1462:1476	In addition, NAM modulated specific bacteria, including Odoribacter, Flexispira, and Bifidobacterium, in the NAM+chronic colitis group.
33748287	7	59	theme	specific	1404:1411	arg1	Flexispira					1446:1455	Flexispira	1446:1455	Flexispira	1446:1455	In addition, NAM modulated specific bacteria, including Odoribacter, Flexispira, and Bifidobacterium, in the NAM+chronic colitis group.
33748287	7	59	theme	specific	1404:1411	arg1	bacteria					1413:1420	specific bacteria	1404:1420	specific bacteria	1404:1420	In addition, NAM modulated specific bacteria, including Odoribacter, Flexispira, and Bifidobacterium, in the NAM+chronic colitis group.
33748287	2	60	from	microbiota	350:359	arg1	mice					400:403	mice	400:403	mice with chronic colitis	400:424	This study was aimed at investigating the effect of NAM on the gut microbiota and short-chain fatty acids (SCFAs) in mice with chronic colitis.
33748287	9	61	with	mice	1840:1843	arg1	colitis					1870:1876	DSS-induced chronic colitis	1850:1876	DSS-induced chronic colitis	1850:1876	Furthermore, NAM also restored the reduction in valeric acid in mice with DSS-induced chronic colitis.
33748287	10	62	from	colitis	1954:1960	arg1	mice					1965:1968	mice	1965:1968	mice	1965:1968	Our results suggest that NAM treatment could alleviate DSS-induced chronic colitis in mice by inhibiting inflammation and regulating the composition and function of gut microbiota.
33748287	5	63	theme	enzyme-linked	737:749	arg1	assay					765:769	enzyme-linked immunosorbent assay	737:769	enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells	737:799	Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment.
33748287	5	63	theme	enzyme-linked	737:749	arg1	ELISA					772:776	ELISA	772:776	ELISA	772:776	Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment.
33748287	6	64	theme	16S	1117:1119	arg1	sequencing					1126:1135	16S rRNA sequencing	1117:1135	16S rRNA sequencing of the large intestinal content	1117:1167	Then, 16S rRNA sequencing of the large intestinal content was performed, and analysis of alpha diversity and beta diversity showed that the richness of the gut microbiota was decreased in the DSS group compared to the control group and restored after NAM treatment.
33748287	5	65	theme	γ	919:919	arg1	level					819:823	the level	815:823	the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A	815:938	Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment.
33748287	5	66	theme	assay	765:769	arg1	analysis					779:786	enzyme-linked immunosorbent assay (ELISA) analysis	737:786	enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells	737:799	Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment.
33748287	5	67	theme	IL-21	922:926	arg1	level					819:823	the level	815:823	the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A	815:938	Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment.
33748287	6	68	theme	NAM	1362:1364	arg1	treatment					1366:1374	NAM treatment	1362:1374	NAM treatment	1362:1374	Then, 16S rRNA sequencing of the large intestinal content was performed, and analysis of alpha diversity and beta diversity showed that the richness of the gut microbiota was decreased in the DSS group compared to the control group and restored after NAM treatment.
33748287	10	69	theme	chronic	1946:1952	arg1	colitis					1954:1960	DSS-induced chronic colitis	1934:1960	DSS-induced chronic colitis in mice	1934:1968	Our results suggest that NAM treatment could alleviate DSS-induced chronic colitis in mice by inhibiting inflammation and regulating the composition and function of gut microbiota.
33748287	5	70	theme	factors	1068:1074	arg1	levels					1048:1053	the levels	1044:1053	the levels of all these factors	1044:1074	Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment.
33748287	8	71	theme	Communities	1543:1553	arg1	Investigation					1526:1538	Phylogenetic Investigation	1513:1538	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis	1513:1611	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis indicated that NAM treatment restored disruptions in the functions of the gut microbiota (replication and repair, cell motility) in mice with DSS-induced colitis.
33748287	5	72	theme	IFN-	913:916	arg1	γ					919:919	IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ	860:919	γ	919:919	Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment.
33748287	5	73	theme	LPL	791:793	arg1	cells					795:799	LPL cells	791:799	LPL cells	791:799	Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment.
33748287	8	74	theme	DSS-induced	1755:1765	arg1	colitis					1767:1773	DSS-induced colitis	1755:1773	DSS-induced colitis	1755:1773	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis indicated that NAM treatment restored disruptions in the functions of the gut microbiota (replication and repair, cell motility) in mice with DSS-induced colitis.
33748287	0	75	theme	Anti-Inflammatory	92:108	arg1	Properties					110:119	Its Anti-Inflammatory Properties	88:119	Its Anti-Inflammatory Properties	88:119	Nicotinamide Ameliorates Dextran Sulfate Sodium-Induced Chronic Colitis in Mice through Its Anti-Inflammatory Properties and Modulates the Gut Microbiota.
33748287	8	76	theme	Unobserved	1576:1585	arg1	PICRUSt					1595:1601	PICRUSt	1595:1601	PICRUSt	1595:1601	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis indicated that NAM treatment restored disruptions in the functions of the gut microbiota (replication and repair, cell motility) in mice with DSS-induced colitis.
33748287	8	76	theme	Unobserved	1576:1585	arg1	States					1587:1592	Unobserved States	1576:1592	Unobserved States (PICRUSt) analysis	1576:1611	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis indicated that NAM treatment restored disruptions in the functions of the gut microbiota (replication and repair, cell motility) in mice with DSS-induced colitis.
33748287	7	77	theme	NAM+chronic	1486:1496	arg1	group					1506:1510	the NAM+chronic colitis group	1482:1510	the NAM+chronic colitis group	1482:1510	In addition, NAM modulated specific bacteria, including Odoribacter, Flexispira, and Bifidobacterium, in the NAM+chronic colitis group.
33748287	6	78	theme	rRNA	1121:1124	arg1	sequencing					1126:1135	16S rRNA sequencing	1117:1135	16S rRNA sequencing of the large intestinal content	1117:1167	Then, 16S rRNA sequencing of the large intestinal content was performed, and analysis of alpha diversity and beta diversity showed that the richness of the gut microbiota was decreased in the DSS group compared to the control group and restored after NAM treatment.
33748287	4	79	theme	weight	627:632	arg1	loss					634:637	weight loss	627:637	weight loss	627:637	NAM treatment ameliorated weight loss and changes in colon length, disease activity index (DAI) score, and histologic scores.
33748287	6	80	theme	intestinal	1150:1159	arg1	content					1161:1167	the large intestinal content	1140:1167	the large intestinal content	1140:1167	Then, 16S rRNA sequencing of the large intestinal content was performed, and analysis of alpha diversity and beta diversity showed that the richness of the gut microbiota was decreased in the DSS group compared to the control group and restored after NAM treatment.
33748287	5	81	link	enzyme-linked	737:749	arg1	assay					765:769	enzyme-linked immunosorbent assay	737:769	enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells	737:799	Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment.
33748287	5	81	link	enzyme-linked	737:749	arg1	ELISA					772:776	ELISA	772:776	ELISA	772:776	Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment.
33748287	4	82	theme	index	685:689	arg1	score					697:701	disease activity index (DAI) score	668:701	disease activity index (DAI) score	668:701	NAM treatment ameliorated weight loss and changes in colon length, disease activity index (DAI) score, and histologic scores.
33748287	8	83	dep	restored	1642:1649	arg1	replication					1703:1713	replication	1703:1713	replication	1703:1713	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis indicated that NAM treatment restored disruptions in the functions of the gut microbiota (replication and repair, cell motility) in mice with DSS-induced colitis.
33748287	8	83	dep	restored	1642:1649	arg1	repair					1719:1724	repair	1719:1724	repair	1719:1724	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis indicated that NAM treatment restored disruptions in the functions of the gut microbiota (replication and repair, cell motility) in mice with DSS-induced colitis.
33748287	8	83	dep	restored	1642:1649	arg1	motility					1732:1739	cell motility	1727:1739	cell motility	1727:1739	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis indicated that NAM treatment restored disruptions in the functions of the gut microbiota (replication and repair, cell motility) in mice with DSS-induced colitis.
33748287	10	84	theme	gut	2044:2046	arg1	microbiota					2048:2057	gut microbiota	2044:2057	gut microbiota	2044:2057	Our results suggest that NAM treatment could alleviate DSS-induced chronic colitis in mice by inhibiting inflammation and regulating the composition and function of gut microbiota.
33748287	2	85	theme	fatty	377:381	arg1	SCFAs					390:394	SCFAs	390:394	SCFAs	390:394	This study was aimed at investigating the effect of NAM on the gut microbiota and short-chain fatty acids (SCFAs) in mice with chronic colitis.
33748287	2	85	theme	fatty	377:381	arg1	acids					383:387	short-chain fatty acids	365:387	short-chain fatty acids (SCFAs)	365:395	This study was aimed at investigating the effect of NAM on the gut microbiota and short-chain fatty acids (SCFAs) in mice with chronic colitis.
33748287	6	86	theme	diversity	1225:1233	arg1	analysis					1188:1195	analysis	1188:1195	analysis of alpha diversity and beta diversity	1188:1233	Then, 16S rRNA sequencing of the large intestinal content was performed, and analysis of alpha diversity and beta diversity showed that the richness of the gut microbiota was decreased in the DSS group compared to the control group and restored after NAM treatment.
33748287	2	87	theme	chronic	410:416	arg1	colitis					418:424	chronic colitis	410:424	chronic colitis	410:424	This study was aimed at investigating the effect of NAM on the gut microbiota and short-chain fatty acids (SCFAs) in mice with chronic colitis.
33748287	5	88	theme	NAM	1096:1098	arg1	treatment					1100:1108	NAM treatment	1096:1108	NAM treatment	1096:1108	Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment.
33748287	0	89	theme	Sulfate	33:39	arg1	Colitis					64:70	Dextran Sulfate Sodium-Induced Chronic Colitis	25:70	Dextran Sulfate Sodium-Induced Chronic Colitis in Mice through Its Anti-Inflammatory Properties	25:119	Nicotinamide Ameliorates Dextran Sulfate Sodium-Induced Chronic Colitis in Mice through Its Anti-Inflammatory Properties and Modulates the Gut Microbiota.
33748287	5	90	theme	chronic	987:993	arg1	group					1003:1007	the chronic colitis group	983:1007	the chronic colitis group compared to the control group	983:1037	Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment.
33748287	8	91	theme	NAM	1628:1630	arg1	treatment					1632:1640	NAM treatment	1628:1640	NAM treatment	1628:1640	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis indicated that NAM treatment restored disruptions in the functions of the gut microbiota (replication and repair, cell motility) in mice with DSS-induced colitis.
33748287	8	92	from	disruptions	1651:1661	arg1	functions					1670:1678	the functions	1666:1678	the functions of the gut microbiota	1666:1700	Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis indicated that NAM treatment restored disruptions in the functions of the gut microbiota (replication and repair, cell motility) in mice with DSS-induced colitis.
33748287	4	93	theme	disease	668:674	arg1	DAI					692:694	DAI	692:694	DAI	692:694	NAM treatment ameliorated weight loss and changes in colon length, disease activity index (DAI) score, and histologic scores.
33748287	4	93	theme	disease	668:674	arg1	index					685:689	disease activity index	668:689	disease activity index (DAI) score	668:701	NAM treatment ameliorated weight loss and changes in colon length, disease activity index (DAI) score, and histologic scores.
33748287	6	94	theme	alpha	1200:1204	arg1	diversity					1206:1214	alpha diversity	1200:1214	alpha diversity	1200:1214	Then, 16S rRNA sequencing of the large intestinal content was performed, and analysis of alpha diversity and beta diversity showed that the richness of the gut microbiota was decreased in the DSS group compared to the control group and restored after NAM treatment.
33748287	6	95	theme	control	1329:1335	arg1	group					1337:1341	the control group	1325:1341	the control group	1325:1341	Then, 16S rRNA sequencing of the large intestinal content was performed, and analysis of alpha diversity and beta diversity showed that the richness of the gut microbiota was decreased in the DSS group compared to the control group and restored after NAM treatment.
33748287	2	96	theme	gut	346:348	arg1	microbiota					350:359	gut microbiota	346:359	gut microbiota	346:359	This study was aimed at investigating the effect of NAM on the gut microbiota and short-chain fatty acids (SCFAs) in mice with chronic colitis.
33748287	9	97	theme	DSS-induced	1850:1860	arg1	colitis					1870:1876	DSS-induced chronic colitis	1850:1876	DSS-induced chronic colitis	1850:1876	Furthermore, NAM also restored the reduction in valeric acid in mice with DSS-induced chronic colitis.
33748287	6	98	theme	microbiota	1271:1280	arg1	richness					1251:1258	the richness	1247:1258	the richness of the gut microbiota	1247:1280	Then, 16S rRNA sequencing of the large intestinal content was performed, and analysis of alpha diversity and beta diversity showed that the richness of the gut microbiota was decreased in the DSS group compared to the control group and restored after NAM treatment.
33748287	3	99	theme	C57BL/6	450:456	arg1	mice					463:466	C57BL/6 male mice	450:466	C57BL/6 male mice	450:466	Colitis was induced in C57BL/6 male mice by administration of 1.5% dextran sulfate sodium (DSS), and the mice were intraperitoneally injected with normal saline (NS) or NAM.
33748287	4	100	from	changes	643:649	arg1	score					697:701	disease activity index (DAI) score	668:701	disease activity index (DAI) score	668:701	NAM treatment ameliorated weight loss and changes in colon length, disease activity index (DAI) score, and histologic scores.
33748287	4	100	from	changes	643:649	arg1	length					660:665	colon length	654:665	colon length	654:665	NAM treatment ameliorated weight loss and changes in colon length, disease activity index (DAI) score, and histologic scores.
33748287	4	100	from	changes	643:649	arg1	scores					719:724	histologic scores	708:724	histologic scores	708:724	NAM treatment ameliorated weight loss and changes in colon length, disease activity index (DAI) score, and histologic scores.
33748287	1	101	theme	nutritional	213:223	arg1	components					225:234	the most important nutritional components	194:234	the most important nutritional components for humans	194:245	Vitamin B (nicotinamide (NAM)), one of the most important nutritional components for humans, exerts anti-inflammatory activity.
33748287	5	102	theme	necrosis	873:880	arg1	γ					919:919	IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ	860:919	γ	919:919	Moreover, enzyme-linked immunosorbent assay (ELISA) analysis of LPL cells revealed that the level of interleukin- (IL-) 6, IL-12p70, IL-1β, tumor necrosis factor- (TNF-) α, interferon- (IFN-) γ, IL-21, and IL-17A was increased, while IL-10 was reduced, in the chronic colitis group compared to the control group, but the levels of all these factors were restored after NAM treatment.
33748287	0	103	theme	Gut	139:141	arg1	Microbiota					143:152	the Gut Microbiota	135:152	the Gut Microbiota	135:152	Nicotinamide Ameliorates Dextran Sulfate Sodium-Induced Chronic Colitis in Mice through Its Anti-Inflammatory Properties and Modulates the Gut Microbiota.
33748287	3	104	theme	1.5	489:491	arg1	%					492:492	%	492:492	%	492:492	Colitis was induced in C57BL/6 male mice by administration of 1.5% dextran sulfate sodium (DSS), and the mice were intraperitoneally injected with normal saline (NS) or NAM.
33748287	4	105	from	loss	634:637	arg1	score					697:701	disease activity index (DAI) score	668:701	disease activity index (DAI) score	668:701	NAM treatment ameliorated weight loss and changes in colon length, disease activity index (DAI) score, and histologic scores.
33748287	4	105	from	loss	634:637	arg1	length					660:665	colon length	654:665	colon length	654:665	NAM treatment ameliorated weight loss and changes in colon length, disease activity index (DAI) score, and histologic scores.
33748287	4	105	from	loss	634:637	arg1	scores					719:724	histologic scores	708:724	histologic scores	708:724	NAM treatment ameliorated weight loss and changes in colon length, disease activity index (DAI) score, and histologic scores.
33496938	20	0	theme	Leucobacter	2157:2167	arg1	sp					2181:2182	the name Leucobacter viscericola sp	2148:2182	the name Leucobacter viscericola sp	2148:2182	nov. for strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T), and the name Leucobacter viscericola sp.
33496938	19	1	theme	strain	1982:1987	arg1	33667T					2031:2036	= KACC 21331T = KCTC 49317T = JCM 33667T	1997:2036	= KACC 21331T = KCTC 49317T = JCM 33667T	1997:2036	nov. for strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T), the name Leucobacter insecticola sp.
33496938	19	1	theme	strain	1982:1987	arg1	HDW9AT					1989:1994	strain HDW9AT	1982:1994	strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T)	1982:2037	nov. for strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T), the name Leucobacter insecticola sp.
33496938	10	2	theme	Leucobacter	1079:1089	arg1	strains					1091:1097	all analyzable Leucobacter strains	1064:1097	all analyzable Leucobacter strains	1064:1097	The isolates showed ANI values of < 76.5% with all analyzable Leucobacter strains in the EzBioCloud database.
33496938	4	3	theme	diving	344:349	arg1	beetles					351:357	the diving beetles	340:357	the diving beetles Cybister lewisianus and Cybister brevis	340:397	Three novel bacterial strains, HDW9AT, HDW9BT, and HDW9CT, isolated from the intestine of the diving beetles Cybister lewisianus and Cybister brevis, were characterized as three novel species using a polyphasic approach.
33496938	4	3	theme	diving	344:349	arg1	Cybister					359:366	Cybister	359:366	Cybister	359:366	Three novel bacterial strains, HDW9AT, HDW9BT, and HDW9CT, isolated from the intestine of the diving beetles Cybister lewisianus and Cybister brevis, were characterized as three novel species using a polyphasic approach.
33496938	4	3	theme	diving	344:349	arg1	Cybister					383:390	Cybister	383:390	Cybister	383:390	Three novel bacterial strains, HDW9AT, HDW9BT, and HDW9CT, isolated from the intestine of the diving beetles Cybister lewisianus and Cybister brevis, were characterized as three novel species using a polyphasic approach.
33496938	12	4	theme	other	1271:1275	arg1	glycolipids					1290:1300	other unidentified glycolipids	1271:1300	other unidentified glycolipids	1271:1300	The polar lipid components were phosphatidylglycerol, diphosphatidylglycerol, and other unidentified glycolipids, phospholipids, and lipids.
33496938	19	5	theme	KACC	1999:2002	arg1	33667T					2031:2036	= KACC 21331T = KCTC 49317T = JCM 33667T	1997:2036	= KACC 21331T = KCTC 49317T = JCM 33667T	1997:2036	nov. for strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T), the name Leucobacter insecticola sp.
33496938	19	5	theme	KACC	1999:2002	arg1	HDW9AT					1989:1994	strain HDW9AT	1982:1994	strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T)	1982:2037	nov. for strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T), the name Leucobacter insecticola sp.
33496938	15	6	theme	isolates	1547:1554	arg1	ribose					1561:1566	ribose	1561:1566	ribose	1561:1566	The whole-cell sugar components of the isolates were ribose, glucose, galactose, and mannose.
33496938	15	6	theme	isolates	1547:1554	arg1	components					1529:1538	The whole-cell sugar components	1508:1538	The whole-cell sugar components of the isolates	1508:1554	The whole-cell sugar components of the isolates were ribose, glucose, galactose, and mannose.
33496938	16	7	theme	wall	1733:1736	arg1	peptidoglycan					1738:1750	the cell wall peptidoglycan	1724:1750	the cell wall peptidoglycan	1724:1750	The isolates harbored L-2,4-diaminobutyric acid, L-serine, L-lysine, L-aspartic acid, glycine, and D-glutamic acid within the cell wall peptidoglycan.
33496938	19	8	theme	49317T	2018:2023	arg1	33667T					2031:2036	= KACC 21331T = KCTC 49317T = JCM 33667T	1997:2036	= KACC 21331T = KCTC 49317T = JCM 33667T	1997:2036	nov. for strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T), the name Leucobacter insecticola sp.
33496938	19	8	theme	49317T	2018:2023	arg1	HDW9AT					1989:1994	strain HDW9AT	1982:1994	strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T)	1982:2037	nov. for strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T), the name Leucobacter insecticola sp.
33496938	3	9	theme	Leucobacter	237:247	arg1	nov.					102:105	nov.	102:105	nov.	102:105	nov., isolated from the intestine of the diving beetles, Cybister brevis and Cybister lewisianus, and emended description of the genus Leucobacter.
33496938	3	9	theme	Leucobacter	237:247	arg1	description					212:222	emended description	204:222	emended description of the genus Leucobacter	204:247	nov., isolated from the intestine of the diving beetles, Cybister brevis and Cybister lewisianus, and emended description of the genus Leucobacter.
33496938	12	10	theme	lipid	1199:1203	arg1	phosphatidylglycerol					1221:1240	phosphatidylglycerol	1221:1240	phosphatidylglycerol	1221:1240	The polar lipid components were phosphatidylglycerol, diphosphatidylglycerol, and other unidentified glycolipids, phospholipids, and lipids.
33496938	12	10	theme	lipid	1199:1203	arg1	components					1205:1214	The polar lipid components	1189:1214	The polar lipid components	1189:1214	The polar lipid components were phosphatidylglycerol, diphosphatidylglycerol, and other unidentified glycolipids, phospholipids, and lipids.
33496938	17	11	dep	strains	1828:1834	arg1	HDW9AT					1836:1841	HDW9AT	1836:1841	HDW9AT	1836:1841	Based on phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses, strains HDW9AT, HDW9BT, and HDW9CT represent three novel species within the genus Leucobacter.
33496938	17	11	dep	strains	1828:1834	arg1	strains					1828:1834	strains	1828:1834	strains HDW9AT, HDW9BT, and HDW9CT	1828:1861	Based on phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses, strains HDW9AT, HDW9BT, and HDW9CT represent three novel species within the genus Leucobacter.
33496938	17	11	dep	strains	1828:1834	arg1	HDW9BT					1844:1849	HDW9BT	1844:1849	HDW9BT	1844:1849	Based on phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses, strains HDW9AT, HDW9BT, and HDW9CT represent three novel species within the genus Leucobacter.
33496938	17	11	dep	strains	1828:1834	arg1	HDW9CT					1856:1861	HDW9CT	1856:1861	HDW9CT	1856:1861	Based on phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses, strains HDW9AT, HDW9BT, and HDW9CT represent three novel species within the genus Leucobacter.
33496938	19	12	theme	name	2044:2047	arg1	sp					2073:2074	the name Leucobacter insecticola sp	2040:2074	the name Leucobacter insecticola sp	2040:2074	nov. for strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T), the name Leucobacter insecticola sp.
33496938	17	13	theme	genotypic	1808:1816	arg1	analyses					1818:1825	phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses	1762:1825	phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses	1762:1825	Based on phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses, strains HDW9AT, HDW9BT, and HDW9CT represent three novel species within the genus Leucobacter.
33496938	6	14	dep	grew	564:567	arg1	pH					588:589	pH 7	588:591	pH 7	588:591	They grew optimally at 30°C (pH 7) in the presence of 0.5% (wt/vol) NaCl.
33496938	16	15	theme	L-2,4-diaminobutyric	1624:1643	arg1	acid					1645:1648	L-2,4-diaminobutyric acid	1624:1648	L-2,4-diaminobutyric acid	1624:1648	The isolates harbored L-2,4-diaminobutyric acid, L-serine, L-lysine, L-aspartic acid, glycine, and D-glutamic acid within the cell wall peptidoglycan.
33496938	20	16	theme	49318T	2122:2127	arg1	HDW9BT					2093:2098	strain HDW9BT	2086:2098	strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T)	2086:2141	nov. for strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T), and the name Leucobacter viscericola sp.
33496938	20	16	theme	49318T	2122:2127	arg1	33668T					2135:2140	= KACC 21332T = KCTC 49318T = JCM 33668T	2101:2140	= KACC 21332T = KCTC 49318T = JCM 33668T	2101:2140	nov. for strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T), and the name Leucobacter viscericola sp.
33496938	20	17	theme	=	2101:2101	arg1	HDW9BT					2093:2098	strain HDW9BT	2086:2098	strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T)	2086:2141	nov. for strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T), and the name Leucobacter viscericola sp.
33496938	20	17	theme	=	2101:2101	arg1	33668T					2135:2140	= KACC 21332T = KCTC 49318T = JCM 33668T	2101:2140	= KACC 21332T = KCTC 49318T = JCM 33668T	2101:2140	nov. for strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T), and the name Leucobacter viscericola sp.
33496938	17	18	theme	novel	1879:1883	arg1	species					1885:1891	three novel species	1873:1891	three novel species within the genus Leucobacter	1873:1920	Based on phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses, strains HDW9AT, HDW9BT, and HDW9CT represent three novel species within the genus Leucobacter.
33496938	10	19	theme	EzBioCloud	1106:1115	arg1	database					1117:1124	the EzBioCloud database	1102:1124	the EzBioCloud database	1102:1124	The isolates showed ANI values of < 76.5% with all analyzable Leucobacter strains in the EzBioCloud database.
33496938	19	20	theme	JCM	2027:2029	arg1	33667T					2031:2036	= KACC 21331T = KCTC 49317T = JCM 33667T	1997:2036	= KACC 21331T = KCTC 49317T = JCM 33667T	1997:2036	nov. for strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T), the name Leucobacter insecticola sp.
33496938	19	20	theme	JCM	2027:2029	arg1	HDW9AT					1989:1994	strain HDW9AT	1982:1994	strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T)	1982:2037	nov. for strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T), the name Leucobacter insecticola sp.
33496938	20	21	theme	JCM	2131:2133	arg1	HDW9BT					2093:2098	strain HDW9BT	2086:2098	strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T)	2086:2141	nov. for strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T), and the name Leucobacter viscericola sp.
33496938	20	21	theme	JCM	2131:2133	arg1	33668T					2135:2140	= KACC 21332T = KCTC 49318T = JCM 33668T	2101:2140	= KACC 21332T = KCTC 49318T = JCM 33668T	2101:2140	nov. for strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T), and the name Leucobacter viscericola sp.
33496938	21	22	theme	KACC	2211:2214	arg1	33669T					2243:2248	= KACC 21333T = KCTC 49319T = JCM 33669T	2209:2248	= KACC 21333T = KCTC 49319T = JCM 33669T	2209:2248	nov. for strain HDW9CT (= KACC 21333T = KCTC 49319T = JCM 33669T).
33496938	21	22	theme	KACC	2211:2214	arg1	HDW9CT					2201:2206	strain HDW9CT	2194:2206	strain HDW9CT (= KACC 21333T = KCTC 49319T = JCM 33669T)	2194:2249	nov. for strain HDW9CT (= KACC 21333T = KCTC 49319T = JCM 33669T).
33496938	3	23	attach	isolated	108:115	arg1	intestine					126:134	the intestine	122:134	the intestine of the diving beetles	122:156	nov., isolated from the intestine of the diving beetles, Cybister brevis and Cybister lewisianus, and emended description of the genus Leucobacter.
33496938	3	23	attach	isolated	108:115	arg2	nov.					102:105	nov.	102:105	nov.	102:105	nov., isolated from the intestine of the diving beetles, Cybister brevis and Cybister lewisianus, and emended description of the genus Leucobacter.
33496938	10	24	theme	analyzable	1068:1077	arg1	strains					1091:1097	all analyzable Leucobacter strains	1064:1097	all analyzable Leucobacter strains	1064:1097	The isolates showed ANI values of < 76.5% with all analyzable Leucobacter strains in the EzBioCloud database.
33496938	19	25	theme	insecticola	2061:2071	arg1	sp					2073:2074	the name Leucobacter insecticola sp	2040:2074	the name Leucobacter insecticola sp	2040:2074	nov. for strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T), the name Leucobacter insecticola sp.
33496938	4	26	attach	isolated	309:316	arg2	HDW9BT					289:294	HDW9BT	289:294	HDW9BT	289:294	Three novel bacterial strains, HDW9AT, HDW9BT, and HDW9CT, isolated from the intestine of the diving beetles Cybister lewisianus and Cybister brevis, were characterized as three novel species using a polyphasic approach.
33496938	4	26	attach	isolated	309:316	arg2	strains					272:278	Three novel bacterial strains	250:278	Three novel bacterial strains	250:278	Three novel bacterial strains, HDW9AT, HDW9BT, and HDW9CT, isolated from the intestine of the diving beetles Cybister lewisianus and Cybister brevis, were characterized as three novel species using a polyphasic approach.
33496938	4	26	attach	isolated	309:316	arg2	HDW9AT					281:286	HDW9AT	281:286	HDW9AT	281:286	Three novel bacterial strains, HDW9AT, HDW9BT, and HDW9CT, isolated from the intestine of the diving beetles Cybister lewisianus and Cybister brevis, were characterized as three novel species using a polyphasic approach.
33496938	4	26	attach	isolated	309:316	arg2	HDW9CT					301:306	HDW9CT	301:306	HDW9CT	301:306	Three novel bacterial strains, HDW9AT, HDW9BT, and HDW9CT, isolated from the intestine of the diving beetles Cybister lewisianus and Cybister brevis, were characterized as three novel species using a polyphasic approach.
33496938	4	26	attach	isolated	309:316	arg1	intestine					327:335	the intestine	323:335	the intestine of the diving beetles Cybister lewisianus and Cybister brevis	323:397	Three novel bacterial strains, HDW9AT, HDW9BT, and HDW9CT, isolated from the intestine of the diving beetles Cybister lewisianus and Cybister brevis, were characterized as three novel species using a polyphasic approach.
33496938	4	26	attach	isolated	309:316	arg2	species					434:440	three novel species	422:440	three novel species using a polyphasic approach	422:468	Three novel bacterial strains, HDW9AT, HDW9BT, and HDW9CT, isolated from the intestine of the diving beetles Cybister lewisianus and Cybister brevis, were characterized as three novel species using a polyphasic approach.
33496938	4	27	theme	polyphasic	450:459	arg1	approach					461:468	a polyphasic approach	448:468	a polyphasic approach	448:468	Three novel bacterial strains, HDW9AT, HDW9BT, and HDW9CT, isolated from the intestine of the diving beetles Cybister lewisianus and Cybister brevis, were characterized as three novel species using a polyphasic approach.
33496938	11	28	theme	C	1147:1147	arg1	content					1149:1155	The genomic DNA G + C content	1127:1155	content	1149:1155	The genomic DNA G + C content of the isolates was 60.3-62.5%.
33496938	17	29	theme	genus	1904:1908	arg1	Leucobacter					1910:1920	the genus Leucobacter	1900:1920	the genus Leucobacter	1900:1920	Based on phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses, strains HDW9AT, HDW9BT, and HDW9CT represent three novel species within the genus Leucobacter.
33496938	4	30	dep	Cybister	359:366	arg1	lewisianus					368:377	Cybister lewisianus	359:377	Cybister lewisianus	359:377	Three novel bacterial strains, HDW9AT, HDW9BT, and HDW9CT, isolated from the intestine of the diving beetles Cybister lewisianus and Cybister brevis, were characterized as three novel species using a polyphasic approach.
33496938	21	31	theme	strain	2194:2199	arg1	33669T					2243:2248	= KACC 21333T = KCTC 49319T = JCM 33669T	2209:2248	= KACC 21333T = KCTC 49319T = JCM 33669T	2209:2248	nov. for strain HDW9CT (= KACC 21333T = KCTC 49319T = JCM 33669T).
33496938	21	31	theme	strain	2194:2199	arg1	HDW9CT					2201:2206	strain HDW9CT	2194:2206	strain HDW9CT (= KACC 21333T = KCTC 49319T = JCM 33669T)	2194:2249	nov. for strain HDW9CT (= KACC 21333T = KCTC 49319T = JCM 33669T).
33496938	14	32	theme	major	1425:1429	arg1	quinone					1443:1449	the major respiratory quinone	1421:1449	the major respiratory quinone	1421:1449	MK-10 was the major respiratory quinone, and MK-7 and MK-11 were the minor respiratory quinones.
33496938	14	32	theme	major	1425:1429	arg1	MK-10					1411:1415	MK-10	1411:1415	MK-10	1411:1415	MK-10 was the major respiratory quinone, and MK-7 and MK-11 were the minor respiratory quinones.
33496938	3	33	dep	Cybister	179:186	arg1	lewisianus					188:197	Cybister lewisianus	179:197	Cybister lewisianus	179:197	nov., isolated from the intestine of the diving beetles, Cybister brevis and Cybister lewisianus, and emended description of the genus Leucobacter.
33496938	20	34	theme	name	2152:2155	arg1	sp					2181:2182	the name Leucobacter viscericola sp	2148:2182	the name Leucobacter viscericola sp	2148:2182	nov. for strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T), and the name Leucobacter viscericola sp.
33496938	4	35	theme	novel	428:432	arg1	strains					272:278	Three novel bacterial strains	250:278	Three novel bacterial strains	250:278	Three novel bacterial strains, HDW9AT, HDW9BT, and HDW9CT, isolated from the intestine of the diving beetles Cybister lewisianus and Cybister brevis, were characterized as three novel species using a polyphasic approach.
33496938	4	35	theme	novel	428:432	arg1	species					434:440	three novel species	422:440	three novel species using a polyphasic approach	422:468	Three novel bacterial strains, HDW9AT, HDW9BT, and HDW9CT, isolated from the intestine of the diving beetles Cybister lewisianus and Cybister brevis, were characterized as three novel species using a polyphasic approach.
33496938	11	36	theme	isolates	1164:1171	arg1	G					1143:1143	The genomic DNA G + C content	1127:1155	G	1143:1143	The genomic DNA G + C content of the isolates was 60.3-62.5%.
33496938	11	36	theme	isolates	1164:1171	arg1	%					1186:1186	60.3-62.5%	1177:1186	60.3-62.5%	1177:1186	The genomic DNA G + C content of the isolates was 60.3-62.5%.
33496938	3	37	theme	diving	143:148	arg1	beetles					150:156	the diving beetles	139:156	the diving beetles	139:156	nov., isolated from the intestine of the diving beetles, Cybister brevis and Cybister lewisianus, and emended description of the genus Leucobacter.
33496938	6	38	theme	NaCl	627:630	arg1	presence					601:608	the presence	597:608	the presence of 0.5% (wt/vol) NaCl	597:630	They grew optimally at 30°C (pH 7) in the presence of 0.5% (wt/vol) NaCl.
33496938	14	39	theme	respiratory	1486:1496	arg1	quinones					1498:1505	the minor respiratory quinones	1476:1505	the minor respiratory quinones	1476:1505	MK-10 was the major respiratory quinone, and MK-7 and MK-11 were the minor respiratory quinones.
33496938	14	39	theme	respiratory	1486:1496	arg1	MK-11					1465:1469	MK-11	1465:1469	MK-11	1465:1469	MK-10 was the major respiratory quinone, and MK-7 and MK-11 were the minor respiratory quinones.
33496938	14	39	theme	respiratory	1486:1496	arg1	MK-7					1456:1459	MK-7	1456:1459	MK-7	1456:1459	MK-10 was the major respiratory quinone, and MK-7 and MK-11 were the minor respiratory quinones.
33496938	9	40	theme	KACC	987:990	arg1	14055T					992:997	L. denitrificans KACC 14055T	970:997	L. denitrificans KACC 14055T	970:997	ANI values for the isolates and the closest taxonomic species, L. denitrificans KACC 14055T, were 72.3-73.1%.
33496938	0	41	theme	Leucobacter	0:10	arg1	sp					26:27	Leucobacter coleopterorum sp	0:27	Leucobacter coleopterorum sp.	0:28	Leucobacter coleopterorum sp.
33496938	17	42	theme	phylogenetic	1762:1773	arg1	analyses					1818:1825	phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses	1762:1825	phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses	1762:1825	Based on phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses, strains HDW9AT, HDW9BT, and HDW9CT represent three novel species within the genus Leucobacter.
33496938	6	43	theme	%	616:616	arg1	NaCl					627:630	0.5% (wt/vol) NaCl	613:630	0.5% (wt/vol) NaCl	613:630	They grew optimally at 30°C (pH 7) in the presence of 0.5% (wt/vol) NaCl.
33496938	13	44	theme	cellular	1340:1347	arg1	acids					1355:1359	The major cellular fatty acids	1330:1359	The major cellular fatty acids	1330:1359	The major cellular fatty acids were anteiso-C15:0, iso-C16:0, and anteiso-C17:0.
33496938	13	44	theme	cellular	1340:1347	arg1	anteiso-C15:0					1366:1378	anteiso-C15:0	1366:1378	anteiso-C15:0	1366:1378	The major cellular fatty acids were anteiso-C15:0, iso-C16:0, and anteiso-C17:0.
33496938	4	45	theme	novel	256:260	arg1	strains					272:278	Three novel bacterial strains	250:278	Three novel bacterial strains	250:278	Three novel bacterial strains, HDW9AT, HDW9BT, and HDW9CT, isolated from the intestine of the diving beetles Cybister lewisianus and Cybister brevis, were characterized as three novel species using a polyphasic approach.
33496938	4	45	theme	novel	256:260	arg1	HDW9BT					289:294	HDW9BT	289:294	HDW9BT	289:294	Three novel bacterial strains, HDW9AT, HDW9BT, and HDW9CT, isolated from the intestine of the diving beetles Cybister lewisianus and Cybister brevis, were characterized as three novel species using a polyphasic approach.
33496938	4	45	theme	novel	256:260	arg1	species					434:440	three novel species	422:440	three novel species using a polyphasic approach	422:468	Three novel bacterial strains, HDW9AT, HDW9BT, and HDW9CT, isolated from the intestine of the diving beetles Cybister lewisianus and Cybister brevis, were characterized as three novel species using a polyphasic approach.
33496938	4	45	theme	novel	256:260	arg1	HDW9CT					301:306	HDW9CT	301:306	HDW9CT	301:306	Three novel bacterial strains, HDW9AT, HDW9BT, and HDW9CT, isolated from the intestine of the diving beetles Cybister lewisianus and Cybister brevis, were characterized as three novel species using a polyphasic approach.
33496938	4	45	theme	novel	256:260	arg1	HDW9AT					281:286	HDW9AT	281:286	HDW9AT	281:286	Three novel bacterial strains, HDW9AT, HDW9BT, and HDW9CT, isolated from the intestine of the diving beetles Cybister lewisianus and Cybister brevis, were characterized as three novel species using a polyphasic approach.
33496938	21	46	theme	49319T	2230:2235	arg1	33669T					2243:2248	= KACC 21333T = KCTC 49319T = JCM 33669T	2209:2248	= KACC 21333T = KCTC 49319T = JCM 33669T	2209:2248	nov. for strain HDW9CT (= KACC 21333T = KCTC 49319T = JCM 33669T).
33496938	21	46	theme	49319T	2230:2235	arg1	HDW9CT					2201:2206	strain HDW9CT	2194:2206	strain HDW9CT (= KACC 21333T = KCTC 49319T = JCM 33669T)	2194:2249	nov. for strain HDW9CT (= KACC 21333T = KCTC 49319T = JCM 33669T).
33496938	8	47	theme	nucleotide	838:847	arg1	identity					849:856	Average nucleotide identity	830:856	Average nucleotide identity (ANI) values among the isolates	830:888	Average nucleotide identity (ANI) values among the isolates were 76.4-84.1%.
33496938	8	47	theme	nucleotide	838:847	arg1	ANI					859:861	ANI	859:861	ANI	859:861	Average nucleotide identity (ANI) values among the isolates were 76.4-84.1%.
33496938	21	48	theme	JCM	2239:2241	arg1	33669T					2243:2248	= KACC 21333T = KCTC 49319T = JCM 33669T	2209:2248	= KACC 21333T = KCTC 49319T = JCM 33669T	2209:2248	nov. for strain HDW9CT (= KACC 21333T = KCTC 49319T = JCM 33669T).
33496938	21	48	theme	JCM	2239:2241	arg1	HDW9CT					2201:2206	strain HDW9CT	2194:2206	strain HDW9CT (= KACC 21333T = KCTC 49319T = JCM 33669T)	2194:2249	nov. for strain HDW9CT (= KACC 21333T = KCTC 49319T = JCM 33669T).
33496938	11	49	theme	genomic	1131:1137	arg1	G					1143:1143	The genomic DNA G + C content	1127:1155	G	1143:1143	The genomic DNA G + C content of the isolates was 60.3-62.5%.
33496938	11	49	theme	genomic	1131:1137	arg1	%					1186:1186	60.3-62.5%	1177:1186	60.3-62.5%	1177:1186	The genomic DNA G + C content of the isolates was 60.3-62.5%.
33496938	16	50	theme	L-aspartic	1671:1680	arg1	acid					1682:1685	L-aspartic acid	1671:1685	L-aspartic acid	1671:1685	The isolates harbored L-2,4-diaminobutyric acid, L-serine, L-lysine, L-aspartic acid, glycine, and D-glutamic acid within the cell wall peptidoglycan.
33496938	15	51	theme	whole-cell	1512:1521	arg1	ribose					1561:1566	ribose	1561:1566	ribose	1561:1566	The whole-cell sugar components of the isolates were ribose, glucose, galactose, and mannose.
33496938	15	51	theme	whole-cell	1512:1521	arg1	components					1529:1538	The whole-cell sugar components	1508:1538	The whole-cell sugar components of the isolates	1508:1554	The whole-cell sugar components of the isolates were ribose, glucose, galactose, and mannose.
33496938	7	52	theme	rRNA	672:675	arg1	sequences					682:690	the 16S rRNA gene sequences	664:690	the 16S rRNA gene sequences	664:690	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that they belong to the genus Leucobacter and are closely related to L. denitrificans M1T8B10T (98.4-98.7% sequence similarity).
33496938	18	53	theme	Leucobacter	1943:1953	arg1	sp					1969:1970	the name Leucobacter coleopterorum sp	1934:1970	the name Leucobacter coleopterorum sp	1934:1970	We propose the name Leucobacter coleopterorum sp.
33496938	4	54	dep	Cybister	383:390	arg1	brevis					392:397	Cybister brevis	383:397	Cybister brevis	383:397	Three novel bacterial strains, HDW9AT, HDW9BT, and HDW9CT, isolated from the intestine of the diving beetles Cybister lewisianus and Cybister brevis, were characterized as three novel species using a polyphasic approach.
33496938	1	55	theme	insecticola	48:58	arg1	sp					60:61	Leucobacter insecticola sp	36:61	Leucobacter insecticola sp	36:61	nov., Leucobacter insecticola sp.
33496938	1	55	theme	insecticola	48:58	arg1	nov.					30:33	nov.	30:33	nov.	30:33	nov., Leucobacter insecticola sp.
33496938	21	56	theme	=	2223:2223	arg1	33669T					2243:2248	= KACC 21333T = KCTC 49319T = JCM 33669T	2209:2248	= KACC 21333T = KCTC 49319T = JCM 33669T	2209:2248	nov. for strain HDW9CT (= KACC 21333T = KCTC 49319T = JCM 33669T).
33496938	21	56	theme	=	2223:2223	arg1	HDW9CT					2201:2206	strain HDW9CT	2194:2206	strain HDW9CT (= KACC 21333T = KCTC 49319T = JCM 33669T)	2194:2249	nov. for strain HDW9CT (= KACC 21333T = KCTC 49319T = JCM 33669T).
33496938	7	57	theme	%	806:806	arg1	similarity					817:826	98.4-98.7% sequence similarity	797:826	98.4-98.7% sequence similarity	797:826	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that they belong to the genus Leucobacter and are closely related to L. denitrificans M1T8B10T (98.4-98.7% sequence similarity).
33496938	7	57	theme	%	806:806	arg1	denitrificans					773:785	L. denitrificans	770:785	L. denitrificans M1T8B10T (98.4-98.7% sequence similarity)	770:827	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that they belong to the genus Leucobacter and are closely related to L. denitrificans M1T8B10T (98.4-98.7% sequence similarity).
33496938	20	58	theme	strain	2086:2091	arg1	HDW9BT					2093:2098	strain HDW9BT	2086:2098	strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T)	2086:2141	nov. for strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T), and the name Leucobacter viscericola sp.
33496938	20	58	theme	strain	2086:2091	arg1	33668T					2135:2140	= KACC 21332T = KCTC 49318T = JCM 33668T	2101:2140	= KACC 21332T = KCTC 49318T = JCM 33668T	2101:2140	nov. for strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T), and the name Leucobacter viscericola sp.
33496938	3	59	theme	emended	204:210	arg1	description					212:222	emended description	204:222	emended description of the genus Leucobacter	204:247	nov., isolated from the intestine of the diving beetles, Cybister brevis and Cybister lewisianus, and emended description of the genus Leucobacter.
33496938	4	60	theme	beetles	351:357	arg1	intestine					327:335	the intestine	323:335	the intestine of the diving beetles Cybister lewisianus and Cybister brevis	323:397	Three novel bacterial strains, HDW9AT, HDW9BT, and HDW9CT, isolated from the intestine of the diving beetles Cybister lewisianus and Cybister brevis, were characterized as three novel species using a polyphasic approach.
33496938	7	61	theme	Phylogenetic	633:644	arg1	analysis					646:653	Phylogenetic analysis	633:653	Phylogenetic analysis based on the 16S rRNA gene sequences	633:690	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that they belong to the genus Leucobacter and are closely related to L. denitrificans M1T8B10T (98.4-98.7% sequence similarity).
33496938	19	62	theme	=	1997:1997	arg1	33667T					2031:2036	= KACC 21331T = KCTC 49317T = JCM 33667T	1997:2036	= KACC 21331T = KCTC 49317T = JCM 33667T	1997:2036	nov. for strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T), the name Leucobacter insecticola sp.
33496938	19	62	theme	=	1997:1997	arg1	HDW9AT					1989:1994	strain HDW9AT	1982:1994	strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T)	1982:2037	nov. for strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T), the name Leucobacter insecticola sp.
33496938	9	63	theme	closest	943:949	arg1	%					1014:1014	72.3-73.1%	1005:1014	72.3-73.1%	1005:1014	ANI values for the isolates and the closest taxonomic species, L. denitrificans KACC 14055T, were 72.3-73.1%.
33496938	9	63	theme	closest	943:949	arg1	species					961:967	the closest taxonomic species	939:967	the closest taxonomic species	939:967	ANI values for the isolates and the closest taxonomic species, L. denitrificans KACC 14055T, were 72.3-73.1%.
33496938	16	64	theme	cell	1728:1731	arg1	peptidoglycan					1738:1750	the cell wall peptidoglycan	1724:1750	the cell wall peptidoglycan	1724:1750	The isolates harbored L-2,4-diaminobutyric acid, L-serine, L-lysine, L-aspartic acid, glycine, and D-glutamic acid within the cell wall peptidoglycan.
33496938	3	65	theme	genus	231:235	arg1	Leucobacter					237:247	the genus Leucobacter	227:247	the genus Leucobacter	227:247	nov., isolated from the intestine of the diving beetles, Cybister brevis and Cybister lewisianus, and emended description of the genus Leucobacter.
33496938	12	66	theme	unidentified	1277:1288	arg1	glycolipids					1290:1300	other unidentified glycolipids	1271:1300	other unidentified glycolipids	1271:1300	The polar lipid components were phosphatidylglycerol, diphosphatidylglycerol, and other unidentified glycolipids, phospholipids, and lipids.
33496938	10	67	theme	ANI	1037:1039	arg1	values					1041:1046	ANI values	1037:1046	ANI values of < 76.5%	1037:1057	The isolates showed ANI values of < 76.5% with all analyzable Leucobacter strains in the EzBioCloud database.
33496938	17	68	theme	phenotypic	1776:1785	arg1	analyses					1818:1825	phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses	1762:1825	phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses	1762:1825	Based on phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses, strains HDW9AT, HDW9BT, and HDW9CT represent three novel species within the genus Leucobacter.
33496938	5	69	dep	Gram-staining-positive	489:510	arg1	Gram-staining-positive					489:510	Gram-staining-positive	489:510	Gram-staining-positive	489:510	The isolates were Gram-staining-positive, strictly aerobic, non-motile, and rod-shaped.
33496938	5	69	dep	Gram-staining-positive	489:510	arg1	aerobic					522:528	aerobic	522:528	aerobic	522:528	The isolates were Gram-staining-positive, strictly aerobic, non-motile, and rod-shaped.
33496938	5	69	dep	Gram-staining-positive	489:510	arg1	isolates					475:482	The isolates	471:482	The isolates	471:482	The isolates were Gram-staining-positive, strictly aerobic, non-motile, and rod-shaped.
33496938	19	70	theme	KCTC	2013:2016	arg1	33667T					2031:2036	= KACC 21331T = KCTC 49317T = JCM 33667T	1997:2036	= KACC 21331T = KCTC 49317T = JCM 33667T	1997:2036	nov. for strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T), the name Leucobacter insecticola sp.
33496938	19	70	theme	KCTC	2013:2016	arg1	HDW9AT					1989:1994	strain HDW9AT	1982:1994	strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T)	1982:2037	nov. for strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T), the name Leucobacter insecticola sp.
33496938	14	71	theme	respiratory	1431:1441	arg1	quinone					1443:1449	the major respiratory quinone	1421:1449	the major respiratory quinone	1421:1449	MK-10 was the major respiratory quinone, and MK-7 and MK-11 were the minor respiratory quinones.
33496938	14	71	theme	respiratory	1431:1441	arg1	MK-10					1411:1415	MK-10	1411:1415	MK-10	1411:1415	MK-10 was the major respiratory quinone, and MK-7 and MK-11 were the minor respiratory quinones.
33496938	17	72	theme	chemotaxonomic	1788:1801	arg1	analyses					1818:1825	phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses	1762:1825	phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses	1762:1825	Based on phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses, strains HDW9AT, HDW9BT, and HDW9CT represent three novel species within the genus Leucobacter.
33496938	19	73	theme	21331T	2004:2009	arg1	33667T					2031:2036	= KACC 21331T = KCTC 49317T = JCM 33667T	1997:2036	= KACC 21331T = KCTC 49317T = JCM 33667T	1997:2036	nov. for strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T), the name Leucobacter insecticola sp.
33496938	19	73	theme	21331T	2004:2009	arg1	HDW9AT					1989:1994	strain HDW9AT	1982:1994	strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T)	1982:2037	nov. for strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T), the name Leucobacter insecticola sp.
33496938	9	74	theme	taxonomic	951:959	arg1	%					1014:1014	72.3-73.1%	1005:1014	72.3-73.1%	1005:1014	ANI values for the isolates and the closest taxonomic species, L. denitrificans KACC 14055T, were 72.3-73.1%.
33496938	9	74	theme	taxonomic	951:959	arg1	species					961:967	the closest taxonomic species	939:967	the closest taxonomic species	939:967	ANI values for the isolates and the closest taxonomic species, L. denitrificans KACC 14055T, were 72.3-73.1%.
33496938	12	75	theme	polar	1193:1197	arg1	phosphatidylglycerol					1221:1240	phosphatidylglycerol	1221:1240	phosphatidylglycerol	1221:1240	The polar lipid components were phosphatidylglycerol, diphosphatidylglycerol, and other unidentified glycolipids, phospholipids, and lipids.
33496938	12	75	theme	polar	1193:1197	arg1	components					1205:1214	The polar lipid components	1189:1214	The polar lipid components	1189:1214	The polar lipid components were phosphatidylglycerol, diphosphatidylglycerol, and other unidentified glycolipids, phospholipids, and lipids.
33496938	9	76	theme	ANI	907:909	arg1	values					911:916	ANI values	907:916	ANI values for the isolates	907:933	ANI values for the isolates and the closest taxonomic species, L. denitrificans KACC 14055T, were 72.3-73.1%.
33496938	2	77	theme	viscericola	86:96	arg1	sp					98:99	viscericola sp	86:99	viscericola sp	86:99	nov., and Leucobacter viscericola sp.
33496938	19	78	theme	Leucobacter	2049:2059	arg1	sp					2073:2074	the name Leucobacter insecticola sp	2040:2074	the name Leucobacter insecticola sp	2040:2074	nov. for strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T), the name Leucobacter insecticola sp.
33496938	19	79	theme	=	2025:2025	arg1	33667T					2031:2036	= KACC 21331T = KCTC 49317T = JCM 33667T	1997:2036	= KACC 21331T = KCTC 49317T = JCM 33667T	1997:2036	nov. for strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T), the name Leucobacter insecticola sp.
33496938	19	79	theme	=	2025:2025	arg1	HDW9AT					1989:1994	strain HDW9AT	1982:1994	strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T)	1982:2037	nov. for strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T), the name Leucobacter insecticola sp.
33496938	20	80	theme	KCTC	2117:2120	arg1	HDW9BT					2093:2098	strain HDW9BT	2086:2098	strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T)	2086:2141	nov. for strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T), and the name Leucobacter viscericola sp.
33496938	20	80	theme	KCTC	2117:2120	arg1	33668T					2135:2140	= KACC 21332T = KCTC 49318T = JCM 33668T	2101:2140	= KACC 21332T = KCTC 49318T = JCM 33668T	2101:2140	nov. for strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T), and the name Leucobacter viscericola sp.
33496938	20	81	theme	=	2129:2129	arg1	HDW9BT					2093:2098	strain HDW9BT	2086:2098	strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T)	2086:2141	nov. for strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T), and the name Leucobacter viscericola sp.
33496938	20	81	theme	=	2129:2129	arg1	33668T					2135:2140	= KACC 21332T = KCTC 49318T = JCM 33668T	2101:2140	= KACC 21332T = KCTC 49318T = JCM 33668T	2101:2140	nov. for strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T), and the name Leucobacter viscericola sp.
33496938	16	82	theme	D-glutamic	1701:1710	arg1	acid					1712:1715	D-glutamic acid	1701:1715	D-glutamic acid	1701:1715	The isolates harbored L-2,4-diaminobutyric acid, L-serine, L-lysine, L-aspartic acid, glycine, and D-glutamic acid within the cell wall peptidoglycan.
33496938	21	83	theme	21333T	2216:2221	arg1	33669T					2243:2248	= KACC 21333T = KCTC 49319T = JCM 33669T	2209:2248	= KACC 21333T = KCTC 49319T = JCM 33669T	2209:2248	nov. for strain HDW9CT (= KACC 21333T = KCTC 49319T = JCM 33669T).
33496938	21	83	theme	21333T	2216:2221	arg1	HDW9CT					2201:2206	strain HDW9CT	2194:2206	strain HDW9CT (= KACC 21333T = KCTC 49319T = JCM 33669T)	2194:2249	nov. for strain HDW9CT (= KACC 21333T = KCTC 49319T = JCM 33669T).
33496938	20	84	theme	KACC	2103:2106	arg1	HDW9BT					2093:2098	strain HDW9BT	2086:2098	strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T)	2086:2141	nov. for strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T), and the name Leucobacter viscericola sp.
33496938	20	84	theme	KACC	2103:2106	arg1	33668T					2135:2140	= KACC 21332T = KCTC 49318T = JCM 33668T	2101:2140	= KACC 21332T = KCTC 49318T = JCM 33668T	2101:2140	nov. for strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T), and the name Leucobacter viscericola sp.
33496938	3	85	dep	Cybister	159:166	arg1	brevis					168:173	Cybister brevis	159:173	Cybister brevis	159:173	nov., isolated from the intestine of the diving beetles, Cybister brevis and Cybister lewisianus, and emended description of the genus Leucobacter.
33496938	21	86	theme	KCTC	2225:2228	arg1	33669T					2243:2248	= KACC 21333T = KCTC 49319T = JCM 33669T	2209:2248	= KACC 21333T = KCTC 49319T = JCM 33669T	2209:2248	nov. for strain HDW9CT (= KACC 21333T = KCTC 49319T = JCM 33669T).
33496938	21	86	theme	KCTC	2225:2228	arg1	HDW9CT					2201:2206	strain HDW9CT	2194:2206	strain HDW9CT (= KACC 21333T = KCTC 49319T = JCM 33669T)	2194:2249	nov. for strain HDW9CT (= KACC 21333T = KCTC 49319T = JCM 33669T).
33496938	2	87	dep	sp	98:99	arg1	nov.					64:67	nov.	64:67	nov.	64:67	nov., and Leucobacter viscericola sp.
33496938	2	87	dep	sp	98:99	arg1	Leucobacter					74:84	Leucobacter	74:84	Leucobacter	74:84	nov., and Leucobacter viscericola sp.
33496938	3	88	theme	beetles	150:156	arg1	intestine					126:134	the intestine	122:134	the intestine of the diving beetles	122:156	nov., isolated from the intestine of the diving beetles, Cybister brevis and Cybister lewisianus, and emended description of the genus Leucobacter.
33496938	4	89	dep	beetles	351:357	arg1	beetles					351:357	the diving beetles	340:357	the diving beetles Cybister lewisianus and Cybister brevis	340:397	Three novel bacterial strains, HDW9AT, HDW9BT, and HDW9CT, isolated from the intestine of the diving beetles Cybister lewisianus and Cybister brevis, were characterized as three novel species using a polyphasic approach.
33496938	4	89	dep	beetles	351:357	arg1	Cybister					359:366	Cybister	359:366	Cybister	359:366	Three novel bacterial strains, HDW9AT, HDW9BT, and HDW9CT, isolated from the intestine of the diving beetles Cybister lewisianus and Cybister brevis, were characterized as three novel species using a polyphasic approach.
33496938	4	89	dep	beetles	351:357	arg1	Cybister					383:390	Cybister	383:390	Cybister	383:390	Three novel bacterial strains, HDW9AT, HDW9BT, and HDW9CT, isolated from the intestine of the diving beetles Cybister lewisianus and Cybister brevis, were characterized as three novel species using a polyphasic approach.
33496938	4	90	theme	bacterial	262:270	arg1	strains					272:278	Three novel bacterial strains	250:278	Three novel bacterial strains	250:278	Three novel bacterial strains, HDW9AT, HDW9BT, and HDW9CT, isolated from the intestine of the diving beetles Cybister lewisianus and Cybister brevis, were characterized as three novel species using a polyphasic approach.
33496938	4	90	theme	bacterial	262:270	arg1	HDW9BT					289:294	HDW9BT	289:294	HDW9BT	289:294	Three novel bacterial strains, HDW9AT, HDW9BT, and HDW9CT, isolated from the intestine of the diving beetles Cybister lewisianus and Cybister brevis, were characterized as three novel species using a polyphasic approach.
33496938	4	90	theme	bacterial	262:270	arg1	species					434:440	three novel species	422:440	three novel species using a polyphasic approach	422:468	Three novel bacterial strains, HDW9AT, HDW9BT, and HDW9CT, isolated from the intestine of the diving beetles Cybister lewisianus and Cybister brevis, were characterized as three novel species using a polyphasic approach.
33496938	4	90	theme	bacterial	262:270	arg1	HDW9CT					301:306	HDW9CT	301:306	HDW9CT	301:306	Three novel bacterial strains, HDW9AT, HDW9BT, and HDW9CT, isolated from the intestine of the diving beetles Cybister lewisianus and Cybister brevis, were characterized as three novel species using a polyphasic approach.
33496938	4	90	theme	bacterial	262:270	arg1	HDW9AT					281:286	HDW9AT	281:286	HDW9AT	281:286	Three novel bacterial strains, HDW9AT, HDW9BT, and HDW9CT, isolated from the intestine of the diving beetles Cybister lewisianus and Cybister brevis, were characterized as three novel species using a polyphasic approach.
33496938	21	91	theme	=	2209:2209	arg1	33669T					2243:2248	= KACC 21333T = KCTC 49319T = JCM 33669T	2209:2248	= KACC 21333T = KCTC 49319T = JCM 33669T	2209:2248	nov. for strain HDW9CT (= KACC 21333T = KCTC 49319T = JCM 33669T).
33496938	21	91	theme	=	2209:2209	arg1	HDW9CT					2201:2206	strain HDW9CT	2194:2206	strain HDW9CT (= KACC 21333T = KCTC 49319T = JCM 33669T)	2194:2249	nov. for strain HDW9CT (= KACC 21333T = KCTC 49319T = JCM 33669T).
33496938	7	92	theme	gene	677:680	arg1	sequences					682:690	the 16S rRNA gene sequences	664:690	the 16S rRNA gene sequences	664:690	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that they belong to the genus Leucobacter and are closely related to L. denitrificans M1T8B10T (98.4-98.7% sequence similarity).
33496938	9	93	theme	denitrificans	973:985	arg1	14055T					992:997	L. denitrificans KACC 14055T	970:997	L. denitrificans KACC 14055T	970:997	ANI values for the isolates and the closest taxonomic species, L. denitrificans KACC 14055T, were 72.3-73.1%.
33496938	0	94	theme	coleopterorum	12:24	arg1	sp					26:27	Leucobacter coleopterorum sp	0:27	Leucobacter coleopterorum sp.	0:28	Leucobacter coleopterorum sp.
33496938	14	95	theme	minor	1480:1484	arg1	quinones					1498:1505	the minor respiratory quinones	1476:1505	the minor respiratory quinones	1476:1505	MK-10 was the major respiratory quinone, and MK-7 and MK-11 were the minor respiratory quinones.
33496938	14	95	theme	minor	1480:1484	arg1	MK-11					1465:1469	MK-11	1465:1469	MK-11	1465:1469	MK-10 was the major respiratory quinone, and MK-7 and MK-11 were the minor respiratory quinones.
33496938	14	95	theme	minor	1480:1484	arg1	MK-7					1456:1459	MK-7	1456:1459	MK-7	1456:1459	MK-10 was the major respiratory quinone, and MK-7 and MK-11 were the minor respiratory quinones.
33496938	7	96	theme	genus	725:729	arg1	Leucobacter					731:741	the genus Leucobacter	721:741	the genus Leucobacter	721:741	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that they belong to the genus Leucobacter and are closely related to L. denitrificans M1T8B10T (98.4-98.7% sequence similarity).
33496938	6	97	dep	%	616:616	arg1	wt/vol					619:624	wt/vol	619:624	wt/vol	619:624	They grew optimally at 30°C (pH 7) in the presence of 0.5% (wt/vol) NaCl.
33496938	20	98	theme	viscericola	2169:2179	arg1	sp					2181:2182	the name Leucobacter viscericola sp	2148:2182	the name Leucobacter viscericola sp	2148:2182	nov. for strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T), and the name Leucobacter viscericola sp.
33496938	6	99	theme	0.5	613:615	arg1	%					616:616	%	616:616	%	616:616	They grew optimally at 30°C (pH 7) in the presence of 0.5% (wt/vol) NaCl.
33496938	13	100	theme	fatty	1349:1353	arg1	acids					1355:1359	The major cellular fatty acids	1330:1359	The major cellular fatty acids	1330:1359	The major cellular fatty acids were anteiso-C15:0, iso-C16:0, and anteiso-C17:0.
33496938	13	100	theme	fatty	1349:1353	arg1	anteiso-C15:0					1366:1378	anteiso-C15:0	1366:1378	anteiso-C15:0	1366:1378	The major cellular fatty acids were anteiso-C15:0, iso-C16:0, and anteiso-C17:0.
33496938	9	101	theme	L.	970:971	arg1	14055T					992:997	L. denitrificans KACC 14055T	970:997	L. denitrificans KACC 14055T	970:997	ANI values for the isolates and the closest taxonomic species, L. denitrificans KACC 14055T, were 72.3-73.1%.
33496938	21	102	theme	=	2237:2237	arg1	33669T					2243:2248	= KACC 21333T = KCTC 49319T = JCM 33669T	2209:2248	= KACC 21333T = KCTC 49319T = JCM 33669T	2209:2248	nov. for strain HDW9CT (= KACC 21333T = KCTC 49319T = JCM 33669T).
33496938	21	102	theme	=	2237:2237	arg1	HDW9CT					2201:2206	strain HDW9CT	2194:2206	strain HDW9CT (= KACC 21333T = KCTC 49319T = JCM 33669T)	2194:2249	nov. for strain HDW9CT (= KACC 21333T = KCTC 49319T = JCM 33669T).
33496938	20	103	theme	=	2115:2115	arg1	HDW9BT					2093:2098	strain HDW9BT	2086:2098	strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T)	2086:2141	nov. for strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T), and the name Leucobacter viscericola sp.
33496938	20	103	theme	=	2115:2115	arg1	33668T					2135:2140	= KACC 21332T = KCTC 49318T = JCM 33668T	2101:2140	= KACC 21332T = KCTC 49318T = JCM 33668T	2101:2140	nov. for strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T), and the name Leucobacter viscericola sp.
33496938	15	104	theme	sugar	1523:1527	arg1	ribose					1561:1566	ribose	1561:1566	ribose	1561:1566	The whole-cell sugar components of the isolates were ribose, glucose, galactose, and mannose.
33496938	15	104	theme	sugar	1523:1527	arg1	components					1529:1538	The whole-cell sugar components	1508:1538	The whole-cell sugar components of the isolates	1508:1554	The whole-cell sugar components of the isolates were ribose, glucose, galactose, and mannose.
33496938	8	105	theme	Average	830:836	arg1	identity					849:856	Average nucleotide identity	830:856	Average nucleotide identity (ANI) values among the isolates	830:888	Average nucleotide identity (ANI) values among the isolates were 76.4-84.1%.
33496938	8	105	theme	Average	830:836	arg1	ANI					859:861	ANI	859:861	ANI	859:861	Average nucleotide identity (ANI) values among the isolates were 76.4-84.1%.
33496938	19	106	theme	=	2011:2011	arg1	33667T					2031:2036	= KACC 21331T = KCTC 49317T = JCM 33667T	1997:2036	= KACC 21331T = KCTC 49317T = JCM 33667T	1997:2036	nov. for strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T), the name Leucobacter insecticola sp.
33496938	19	106	theme	=	2011:2011	arg1	HDW9AT					1989:1994	strain HDW9AT	1982:1994	strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T)	1982:2037	nov. for strain HDW9AT (= KACC 21331T = KCTC 49317T = JCM 33667T), the name Leucobacter insecticola sp.
33496938	11	107	theme	DNA	1139:1141	arg1	G					1143:1143	The genomic DNA G + C content	1127:1155	G	1143:1143	The genomic DNA G + C content of the isolates was 60.3-62.5%.
33496938	11	107	theme	DNA	1139:1141	arg1	%					1186:1186	60.3-62.5%	1177:1186	60.3-62.5%	1177:1186	The genomic DNA G + C content of the isolates was 60.3-62.5%.
33496938	18	108	theme	coleopterorum	1955:1967	arg1	sp					1969:1970	the name Leucobacter coleopterorum sp	1934:1970	the name Leucobacter coleopterorum sp	1934:1970	We propose the name Leucobacter coleopterorum sp.
33496938	8	109	theme	identity	849:856	arg1	values					864:869	Average nucleotide identity (ANI) values	830:869	Average nucleotide identity (ANI) values among the isolates	830:888	Average nucleotide identity (ANI) values among the isolates were 76.4-84.1%.
33496938	13	110	theme	major	1334:1338	arg1	acids					1355:1359	The major cellular fatty acids	1330:1359	The major cellular fatty acids	1330:1359	The major cellular fatty acids were anteiso-C15:0, iso-C16:0, and anteiso-C17:0.
33496938	13	110	theme	major	1334:1338	arg1	anteiso-C15:0					1366:1378	anteiso-C15:0	1366:1378	anteiso-C15:0	1366:1378	The major cellular fatty acids were anteiso-C15:0, iso-C16:0, and anteiso-C17:0.
33496938	7	111	theme	sequence	808:815	arg1	similarity					817:826	98.4-98.7% sequence similarity	797:826	98.4-98.7% sequence similarity	797:826	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that they belong to the genus Leucobacter and are closely related to L. denitrificans M1T8B10T (98.4-98.7% sequence similarity).
33496938	7	111	theme	sequence	808:815	arg1	denitrificans					773:785	L. denitrificans	770:785	L. denitrificans M1T8B10T (98.4-98.7% sequence similarity)	770:827	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that they belong to the genus Leucobacter and are closely related to L. denitrificans M1T8B10T (98.4-98.7% sequence similarity).
33496938	10	112	theme	%	1057:1057	arg1	values					1041:1046	ANI values	1037:1046	ANI values of < 76.5%	1037:1057	The isolates showed ANI values of < 76.5% with all analyzable Leucobacter strains in the EzBioCloud database.
33496938	18	113	theme	name	1938:1941	arg1	sp					1969:1970	the name Leucobacter coleopterorum sp	1934:1970	the name Leucobacter coleopterorum sp	1934:1970	We propose the name Leucobacter coleopterorum sp.
33496938	1	114	theme	Leucobacter	36:46	arg1	sp					60:61	Leucobacter insecticola sp	36:61	Leucobacter insecticola sp	36:61	nov., Leucobacter insecticola sp.
33496938	1	114	theme	Leucobacter	36:46	arg1	nov.					30:33	nov.	30:33	nov.	30:33	nov., Leucobacter insecticola sp.
33496938	20	115	theme	21332T	2108:2113	arg1	HDW9BT					2093:2098	strain HDW9BT	2086:2098	strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T)	2086:2141	nov. for strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T), and the name Leucobacter viscericola sp.
33496938	20	115	theme	21332T	2108:2113	arg1	33668T					2135:2140	= KACC 21332T = KCTC 49318T = JCM 33668T	2101:2140	= KACC 21332T = KCTC 49318T = JCM 33668T	2101:2140	nov. for strain HDW9BT (= KACC 21332T = KCTC 49318T = JCM 33668T), and the name Leucobacter viscericola sp.
33496938	7	116	theme	98.4-98.7	797:805	arg1	%					806:806	%	806:806	%	806:806	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that they belong to the genus Leucobacter and are closely related to L. denitrificans M1T8B10T (98.4-98.7% sequence similarity).
33496938	7	117	theme	16S	668:670	arg1	sequences					682:690	the 16S rRNA gene sequences	664:690	the 16S rRNA gene sequences	664:690	Phylogenetic analysis based on the 16S rRNA gene sequences revealed that they belong to the genus Leucobacter and are closely related to L. denitrificans M1T8B10T (98.4-98.7% sequence similarity).
32996878	10	0	theme	DDH	1493:1495	arg1	value					1506:1510	The in silico DDH estimate value	1479:1510	The in silico DDH estimate value between the respective type strain TMW 2.1612T and TMW 2.1615T and the most closely related type strain of Lactococcus piscium	1479:1637	The in silico DDH estimate value between the respective type strain TMW 2.1612T and TMW 2.1615T and the most closely related type strain of Lactococcus piscium was only 59.9 and 48.9 %, respectively, and 51.1 % between TMW 2.1612T and TMW 2.1615T. Peptidoglycan type of strain TMW 2.1612T is Lys-Thr-Ala and major fatty acids are summed feature 8 and C16 : 0.
32996878	10	0	theme	DDH	1493:1495	arg1	59.9					1648:1651	59.9	1648:1651	59.9	1648:1651	The in silico DDH estimate value between the respective type strain TMW 2.1612T and TMW 2.1615T and the most closely related type strain of Lactococcus piscium was only 59.9 and 48.9 %, respectively, and 51.1 % between TMW 2.1612T and TMW 2.1615T. Peptidoglycan type of strain TMW 2.1612T is Lys-Thr-Ala and major fatty acids are summed feature 8 and C16 : 0.
32996878	6	1	theme	TMW	872:874	arg1	2.1614					888:893	TMW 2.1615T/TMW 2.1614	872:893	TMW 2.1615T/TMW 2.1614	872:893	Results indicated that they represented two different novel species with two strains, (TMW 2.1612T/TMW 2.1613 and TMW 2.1615T/TMW 2.1614), respectively.
32996878	3	2	theme	modified-atmosphere	203:221	arg1	microbiome					189:198	the spoilage microbiome	176:198	the spoilage microbiome of modified-atmosphere packaged beef from Germany	176:248	As part of a study investigating the spoilage microbiome of modified-atmosphere packaged beef from Germany, four novel strains of lactic acid bacteria were isolated and subsequently taxonomically characterized by a polyphasic approach, which revealed that they could not be assigned to known species.
32996878	10	3	theme	type	1535:1538	arg1	strain					1540:1545	the respective type strain	1520:1545	the respective type strain TMW 2.1612T and TMW 2.1615T	1520:1573	The in silico DDH estimate value between the respective type strain TMW 2.1612T and TMW 2.1615T and the most closely related type strain of Lactococcus piscium was only 59.9 and 48.9 %, respectively, and 51.1 % between TMW 2.1612T and TMW 2.1615T. Peptidoglycan type of strain TMW 2.1612T is Lys-Thr-Ala and major fatty acids are summed feature 8 and C16 : 0.
32996878	6	4	dep	strains	835:841	arg1	2.1613					861:866	TMW 2.1612T/TMW 2.1613	845:866	TMW 2.1612T/TMW 2.1613	845:866	Results indicated that they represented two different novel species with two strains, (TMW 2.1612T/TMW 2.1613 and TMW 2.1615T/TMW 2.1614), respectively.
32996878	6	4	dep	strains	835:841	arg1	2.1614					888:893	TMW 2.1615T/TMW 2.1614	872:893	TMW 2.1615T/TMW 2.1614	872:893	Results indicated that they represented two different novel species with two strains, (TMW 2.1612T/TMW 2.1613 and TMW 2.1615T/TMW 2.1614), respectively.
32996878	6	5	theme	TMW	845:847	arg1	2.1613					861:866	TMW 2.1612T/TMW 2.1613	845:866	TMW 2.1612T/TMW 2.1613	845:866	Results indicated that they represented two different novel species with two strains, (TMW 2.1612T/TMW 2.1613 and TMW 2.1615T/TMW 2.1614), respectively.
32996878	8	6	theme	16S	1049:1051	arg1	sequences					1063:1071	The 16S rRNA gene sequences	1045:1071	The 16S rRNA gene sequences of TMW 2.1612T and TMW 2.1615T	1045:1102	The 16S rRNA gene sequences of TMW 2.1612T and TMW 2.1615T had a similarity of 99.85 % to each other and a similarity of 99.85 and 99.78 % the most closely related type strain of Lactococcus piscium, respectively.
32996878	8	7	theme	type	1209:1212	arg1	strain					1214:1219	the most closely related type strain	1184:1219	the most closely related type strain of Lactococcus piscium	1184:1242	The 16S rRNA gene sequences of TMW 2.1612T and TMW 2.1615T had a similarity of 99.85 % to each other and a similarity of 99.85 and 99.78 % the most closely related type strain of Lactococcus piscium, respectively.
32996878	3	8	theme	lactic	273:278	arg1	bacteria					285:292	lactic acid bacteria	273:292	lactic acid bacteria	273:292	As part of a study investigating the spoilage microbiome of modified-atmosphere packaged beef from Germany, four novel strains of lactic acid bacteria were isolated and subsequently taxonomically characterized by a polyphasic approach, which revealed that they could not be assigned to known species.
32996878	8	9	theme	gene	1058:1061	arg1	sequences					1063:1071	The 16S rRNA gene sequences	1045:1071	The 16S rRNA gene sequences of TMW 2.1612T and TMW 2.1615T	1045:1102	The 16S rRNA gene sequences of TMW 2.1612T and TMW 2.1615T had a similarity of 99.85 % to each other and a similarity of 99.85 and 99.78 % the most closely related type strain of Lactococcus piscium, respectively.
32996878	15	10	theme	=DSM	2230:2233	arg1	30115T					2249:2254	=DSM 111016T =CECT 30115T	2230:2254	=DSM 111016T =CECT 30115T	2230:2254	The designated respective type strains are TMW 2.1612T (=DSM 111016T =CECT 30115T) and TWM 2.1615T (=DSM 111017T =CECT 30116T).
32996878	15	10	theme	=DSM	2230:2233	arg1	2.1612T					2221:2227	TMW 2.1612T	2217:2227	TMW 2.1612T (=DSM 111016T =CECT 30115T)	2217:2255	The designated respective type strains are TMW 2.1612T (=DSM 111016T =CECT 30115T) and TWM 2.1615T (=DSM 111017T =CECT 30116T).
32996878	10	11	theme	TMW	1698:1700	arg1	2.1612T					1702:1708	TMW 2.1612T and TMW 2.1615T. Peptidoglycan type	1698:1744	2.1612T	1702:1708	The in silico DDH estimate value between the respective type strain TMW 2.1612T and TMW 2.1615T and the most closely related type strain of Lactococcus piscium was only 59.9 and 48.9 %, respectively, and 51.1 % between TMW 2.1612T and TMW 2.1615T. Peptidoglycan type of strain TMW 2.1612T is Lys-Thr-Ala and major fatty acids are summed feature 8 and C16 : 0.
32996878	11	12	theme	2.1615T	1872:1878	arg1	type					1853:1856	Peptidoglycan type	1839:1856	Peptidoglycan type of strain TMW 2.1615T	1839:1878	Peptidoglycan type of strain TMW 2.1615T is Lys-Ala and major fatty acids are C16 : 0, C19 : 0cyclo ω8c and summed feature 8.
32996878	5	13	theme	Morphological	572:584	arg1	analysis					618:625	Morphological, physiological and phylogenetic analysis	572:625	Morphological, physiological and phylogenetic analysis	572:625	Morphological, physiological and phylogenetic analysis revealed a distinct lineage within the genus Lactococcus, with Lactococcus piscium and Lactococcus plantarum as closest relatives.
32996878	9	14	theme	TMW	1450:1452	arg1	2.1612T					1454:1460	TMW 2.1612T	1450:1460	TMW 2.1612T	1450:1460	However, the ANIb value between the respective type strains TMW 2.1612T and TMW 2.1615T, and the type strain of Lactococcus piscium was only 94.3 and 92.0 %, respectively, and 92.9 % between TMW 2.1612T and TMW 2.1615T.
32996878	8	15	theme	piscium	1236:1242	arg1	strain					1214:1219	the most closely related type strain	1184:1219	the most closely related type strain of Lactococcus piscium	1184:1242	The 16S rRNA gene sequences of TMW 2.1612T and TMW 2.1615T had a similarity of 99.85 % to each other and a similarity of 99.85 and 99.78 % the most closely related type strain of Lactococcus piscium, respectively.
32996878	11	16	theme	summed	1947:1952	arg1	feature					1954:1960	summed feature 8	1947:1962	summed feature 8	1947:1962	Peptidoglycan type of strain TMW 2.1615T is Lys-Ala and major fatty acids are C16 : 0, C19 : 0cyclo ω8c and summed feature 8.
32996878	2	17	theme	novel	73:77	arg1	species					79:85	two novel species	69:85	two novel species isolated from modified-atmosphere	69:119	nov., two novel species isolated from modified-atmosphere packaged beef steaks.
32996878	10	18	theme	2.1615T.	1718:1725	arg1	type					1741:1744	TMW 2.1612T and TMW 2.1615T. Peptidoglycan type	1698:1744	type	1741:1744	The in silico DDH estimate value between the respective type strain TMW 2.1612T and TMW 2.1615T and the most closely related type strain of Lactococcus piscium was only 59.9 and 48.9 %, respectively, and 51.1 % between TMW 2.1612T and TMW 2.1615T. Peptidoglycan type of strain TMW 2.1612T is Lys-Thr-Ala and major fatty acids are summed feature 8 and C16 : 0.
32996878	5	19	theme	physiological	587:599	arg1	analysis					618:625	Morphological, physiological and phylogenetic analysis	572:625	Morphological, physiological and phylogenetic analysis	572:625	Morphological, physiological and phylogenetic analysis revealed a distinct lineage within the genus Lactococcus, with Lactococcus piscium and Lactococcus plantarum as closest relatives.
32996878	13	20	theme	Lactococcus	2127:2137	arg1	paracarnosus					2139:2150	Lactococcus paracarnosus	2127:2150	Lactococcus paracarnosus	2127:2150	nov and Lactococcus paracarnosus sp.
32996878	3	21	theme	bacteria	285:292	arg1	strains					262:268	four novel strains	251:268	four novel strains of lactic acid bacteria	251:292	As part of a study investigating the spoilage microbiome of modified-atmosphere packaged beef from Germany, four novel strains of lactic acid bacteria were isolated and subsequently taxonomically characterized by a polyphasic approach, which revealed that they could not be assigned to known species.
32996878	3	21	theme	bacteria	285:292	arg1	part					146:149	part	146:149	part of a study investigating the spoilage microbiome of modified-atmosphere packaged beef from Germany	146:248	As part of a study investigating the spoilage microbiome of modified-atmosphere packaged beef from Germany, four novel strains of lactic acid bacteria were isolated and subsequently taxonomically characterized by a polyphasic approach, which revealed that they could not be assigned to known species.
32996878	1	22	dep	sp	59:60	arg1	paracarnosus					46:57	nov. and Lactococcus paracarnosus	25:57	nov. and Lactococcus paracarnosus	25:57	nov. and Lactococcus paracarnosus sp.
32996878	10	23	dep	value	1506:1510	arg1	estimate					1497:1504	estimate	1497:1504	estimate	1497:1504	The in silico DDH estimate value between the respective type strain TMW 2.1612T and TMW 2.1615T and the most closely related type strain of Lactococcus piscium was only 59.9 and 48.9 %, respectively, and 51.1 % between TMW 2.1612T and TMW 2.1615T. Peptidoglycan type of strain TMW 2.1612T is Lys-Thr-Ala and major fatty acids are summed feature 8 and C16 : 0.
32996878	9	24	theme	ANIb	1272:1275	arg1	94.3					1400:1403	94.3	1400:1403	94.3	1400:1403	However, the ANIb value between the respective type strains TMW 2.1612T and TMW 2.1615T, and the type strain of Lactococcus piscium was only 94.3 and 92.0 %, respectively, and 92.9 % between TMW 2.1612T and TMW 2.1615T.
32996878	9	24	theme	ANIb	1272:1275	arg1	value					1277:1281	the ANIb value	1268:1281	the ANIb value between the respective type strains TMW 2.1612T and TMW 2.1615T, and the type strain of Lactococcus piscium	1268:1389	However, the ANIb value between the respective type strains TMW 2.1612T and TMW 2.1615T, and the type strain of Lactococcus piscium was only 94.3 and 92.0 %, respectively, and 92.9 % between TMW 2.1612T and TMW 2.1615T.
32996878	10	25	theme	2.1612T	1760:1766	arg1	type					1741:1744	TMW 2.1612T and TMW 2.1615T. Peptidoglycan type	1698:1744	type	1741:1744	The in silico DDH estimate value between the respective type strain TMW 2.1612T and TMW 2.1615T and the most closely related type strain of Lactococcus piscium was only 59.9 and 48.9 %, respectively, and 51.1 % between TMW 2.1612T and TMW 2.1615T. Peptidoglycan type of strain TMW 2.1612T is Lys-Thr-Ala and major fatty acids are summed feature 8 and C16 : 0.
32996878	10	25	theme	2.1612T	1760:1766	arg1	2.1612T					1702:1708	TMW 2.1612T and TMW 2.1615T. Peptidoglycan type	1698:1744	2.1612T	1702:1708	The in silico DDH estimate value between the respective type strain TMW 2.1612T and TMW 2.1615T and the most closely related type strain of Lactococcus piscium was only 59.9 and 48.9 %, respectively, and 51.1 % between TMW 2.1612T and TMW 2.1615T. Peptidoglycan type of strain TMW 2.1612T is Lys-Thr-Ala and major fatty acids are summed feature 8 and C16 : 0.
32996878	11	26	dep	C16 	1917:1920	arg1	ω8c					1939:1941	ω8c	1939:1941	ω8c	1939:1941	Peptidoglycan type of strain TMW 2.1615T is Lys-Ala and major fatty acids are C16 : 0, C19 : 0cyclo ω8c and summed feature 8.
32996878	11	26	dep	C16 	1917:1920	arg1	feature					1954:1960	summed feature 8	1947:1962	summed feature 8	1947:1962	Peptidoglycan type of strain TMW 2.1615T is Lys-Ala and major fatty acids are C16 : 0, C19 : 0cyclo ω8c and summed feature 8.
32996878	11	26	dep	C16 	1917:1920	arg1	C19 					1926:1929	C19 	1926:1929	C19 	1926:1929	Peptidoglycan type of strain TMW 2.1615T is Lys-Ala and major fatty acids are C16 : 0, C19 : 0cyclo ω8c and summed feature 8.
32996878	10	27	theme	strain	1749:1754	arg1	2.1612T					1760:1766	strain TMW 2.1612T	1749:1766	strain TMW 2.1612T	1749:1766	The in silico DDH estimate value between the respective type strain TMW 2.1612T and TMW 2.1615T and the most closely related type strain of Lactococcus piscium was only 59.9 and 48.9 %, respectively, and 51.1 % between TMW 2.1612T and TMW 2.1615T. Peptidoglycan type of strain TMW 2.1612T is Lys-Thr-Ala and major fatty acids are summed feature 8 and C16 : 0.
32996878	7	28	theme	intraspecies	1015:1026	arg1	differentiation					1028:1042	their intraspecies differentiation	1009:1042	their intraspecies differentiation	1009:1042	The two strains of both novel species shared identical 16S rRNA gene sequences but a MLSA allowed their intraspecies differentiation.
32996878	10	29	dep	in	1483:1484	arg1	silico					1486:1491	silico	1486:1491	silico	1486:1491	The in silico DDH estimate value between the respective type strain TMW 2.1612T and TMW 2.1615T and the most closely related type strain of Lactococcus piscium was only 59.9 and 48.9 %, respectively, and 51.1 % between TMW 2.1612T and TMW 2.1615T. Peptidoglycan type of strain TMW 2.1612T is Lys-Thr-Ala and major fatty acids are summed feature 8 and C16 : 0.
32996878	9	30	theme	respective	1295:1304	arg1	2.1612T					1323:1329	TMW 2.1612T	1319:1329	TMW 2.1612T	1319:1329	However, the ANIb value between the respective type strains TMW 2.1612T and TMW 2.1615T, and the type strain of Lactococcus piscium was only 94.3 and 92.0 %, respectively, and 92.9 % between TMW 2.1612T and TMW 2.1615T.
32996878	9	30	theme	respective	1295:1304	arg1	2.1615T					1339:1345	TMW 2.1615T	1335:1345	TMW 2.1615T	1335:1345	However, the ANIb value between the respective type strains TMW 2.1612T and TMW 2.1615T, and the type strain of Lactococcus piscium was only 94.3 and 92.0 %, respectively, and 92.9 % between TMW 2.1612T and TMW 2.1615T.
32996878	9	30	theme	respective	1295:1304	arg1	strains					1311:1317	the respective type strains	1291:1317	the respective type strains TMW 2.1612T and TMW 2.1615T	1291:1345	However, the ANIb value between the respective type strains TMW 2.1612T and TMW 2.1615T, and the type strain of Lactococcus piscium was only 94.3 and 92.0 %, respectively, and 92.9 % between TMW 2.1612T and TMW 2.1615T.
32996878	15	31	theme	type	2200:2203	arg1	strains					2205:2211	The designated respective type strains	2174:2211	The designated respective type strains	2174:2211	The designated respective type strains are TMW 2.1612T (=DSM 111016T =CECT 30115T) and TWM 2.1615T (=DSM 111017T =CECT 30116T).
32996878	15	31	theme	type	2200:2203	arg1	2.1612T					2221:2227	TMW 2.1612T	2217:2227	TMW 2.1612T (=DSM 111016T =CECT 30115T)	2217:2255	The designated respective type strains are TMW 2.1612T (=DSM 111016T =CECT 30115T) and TWM 2.1615T (=DSM 111017T =CECT 30116T).
32996878	5	32	theme	distinct	638:645	arg1	lineage					647:653	a distinct lineage	636:653	a distinct lineage within the genus Lactococcus	636:682	Morphological, physiological and phylogenetic analysis revealed a distinct lineage within the genus Lactococcus, with Lactococcus piscium and Lactococcus plantarum as closest relatives.
32996878	11	33	theme	major	1895:1899	arg1	acids					1907:1911	major fatty acids	1895:1911	major fatty acids	1895:1911	Peptidoglycan type of strain TMW 2.1615T is Lys-Ala and major fatty acids are C16 : 0, C19 : 0cyclo ω8c and summed feature 8.
32996878	8	34	theme	2.1612T	1080:1086	arg1	sequences					1063:1071	The 16S rRNA gene sequences	1045:1071	The 16S rRNA gene sequences of TMW 2.1612T and TMW 2.1615T	1045:1102	The 16S rRNA gene sequences of TMW 2.1612T and TMW 2.1615T had a similarity of 99.85 % to each other and a similarity of 99.85 and 99.78 % the most closely related type strain of Lactococcus piscium, respectively.
32996878	12	35	theme	Lactococcus	2094:2104	arg1	sp					2115:2116	Lactococcus carnosus sp	2094:2116	Lactococcus carnosus sp	2094:2116	On the basis of polyphasic evidence, the meat isolates represent two novel species of the genus Lactococcus, for which the names Lactococcus carnosus sp.
32996878	8	36	contain	had	1104:1106	arg2	strain					1214:1219	the most closely related type strain	1184:1219	the most closely related type strain of Lactococcus piscium	1184:1242	The 16S rRNA gene sequences of TMW 2.1612T and TMW 2.1615T had a similarity of 99.85 % to each other and a similarity of 99.85 and 99.78 % the most closely related type strain of Lactococcus piscium, respectively.
32996878	8	36	contain	had	1104:1106	arg1	sequences					1063:1071	The 16S rRNA gene sequences	1045:1071	The 16S rRNA gene sequences of TMW 2.1612T and TMW 2.1615T	1045:1102	The 16S rRNA gene sequences of TMW 2.1612T and TMW 2.1615T had a similarity of 99.85 % to each other and a similarity of 99.85 and 99.78 % the most closely related type strain of Lactococcus piscium, respectively.
32996878	15	37	theme	=CECT	2243:2247	arg1	30115T					2249:2254	=DSM 111016T =CECT 30115T	2230:2254	=DSM 111016T =CECT 30115T	2230:2254	The designated respective type strains are TMW 2.1612T (=DSM 111016T =CECT 30115T) and TWM 2.1615T (=DSM 111017T =CECT 30116T).
32996878	15	37	theme	=CECT	2243:2247	arg1	2.1612T					2221:2227	TMW 2.1612T	2217:2227	TMW 2.1612T (=DSM 111016T =CECT 30115T)	2217:2255	The designated respective type strains are TMW 2.1612T (=DSM 111016T =CECT 30115T) and TWM 2.1615T (=DSM 111017T =CECT 30116T).
32996878	8	38	theme	TMW	1092:1094	arg1	2.1615T					1096:1102	TMW 2.1615T	1092:1102	TMW 2.1615T	1092:1102	The 16S rRNA gene sequences of TMW 2.1612T and TMW 2.1615T had a similarity of 99.85 % to each other and a similarity of 99.85 and 99.78 % the most closely related type strain of Lactococcus piscium, respectively.
32996878	5	39	theme	phylogenetic	605:616	arg1	analysis					618:625	Morphological, physiological and phylogenetic analysis	572:625	Morphological, physiological and phylogenetic analysis	572:625	Morphological, physiological and phylogenetic analysis revealed a distinct lineage within the genus Lactococcus, with Lactococcus piscium and Lactococcus plantarum as closest relatives.
32996878	15	40	theme	111017T	2279:2285	arg1	30116T					2293:2298	=DSM 111017T =CECT 30116T	2274:2298	=DSM 111017T =CECT 30116T	2274:2298	The designated respective type strains are TMW 2.1612T (=DSM 111016T =CECT 30115T) and TWM 2.1615T (=DSM 111017T =CECT 30116T).
32996878	15	40	theme	111017T	2279:2285	arg1	2.1615T					2265:2271	TWM 2.1615T	2261:2271	TWM 2.1615T (=DSM 111017T =CECT 30116T)	2261:2299	The designated respective type strains are TMW 2.1612T (=DSM 111016T =CECT 30115T) and TWM 2.1615T (=DSM 111017T =CECT 30116T).
32996878	10	41	theme	respective	1524:1533	arg1	strain					1540:1545	the respective type strain	1520:1545	the respective type strain TMW 2.1612T and TMW 2.1615T	1520:1573	The in silico DDH estimate value between the respective type strain TMW 2.1612T and TMW 2.1615T and the most closely related type strain of Lactococcus piscium was only 59.9 and 48.9 %, respectively, and 51.1 % between TMW 2.1612T and TMW 2.1615T. Peptidoglycan type of strain TMW 2.1612T is Lys-Thr-Ala and major fatty acids are summed feature 8 and C16 : 0.
32996878	12	42	theme	Lactococcus	2061:2071	arg1	species					2040:2046	two novel species	2030:2046	two novel species	2030:2046	On the basis of polyphasic evidence, the meat isolates represent two novel species of the genus Lactococcus, for which the names Lactococcus carnosus sp.
32996878	10	43	theme	related	1596:1602	arg1	strain					1609:1614	the most closely related type strain	1579:1614	the most closely related type strain of Lactococcus piscium	1579:1637	The in silico DDH estimate value between the respective type strain TMW 2.1612T and TMW 2.1615T and the most closely related type strain of Lactococcus piscium was only 59.9 and 48.9 %, respectively, and 51.1 % between TMW 2.1612T and TMW 2.1615T. Peptidoglycan type of strain TMW 2.1612T is Lys-Thr-Ala and major fatty acids are summed feature 8 and C16 : 0.
32996878	0	44	theme	Lactococcus	0:10	arg1	carnosus					12:19	Lactococcus carnosus	0:19	Lactococcus carnosus	0:19	Lactococcus carnosus sp.
32996878	9	45	theme	TMW	1335:1337	arg1	2.1615T					1339:1345	TMW 2.1615T	1335:1345	TMW 2.1615T	1335:1345	However, the ANIb value between the respective type strains TMW 2.1612T and TMW 2.1615T, and the type strain of Lactococcus piscium was only 94.3 and 92.0 %, respectively, and 92.9 % between TMW 2.1612T and TMW 2.1615T.
32996878	9	45	theme	TMW	1335:1337	arg1	strains					1311:1317	the respective type strains	1291:1317	the respective type strains TMW 2.1612T and TMW 2.1615T	1291:1345	However, the ANIb value between the respective type strains TMW 2.1612T and TMW 2.1615T, and the type strain of Lactococcus piscium was only 94.3 and 92.0 %, respectively, and 92.9 % between TMW 2.1612T and TMW 2.1615T.
32996878	7	46	theme	rRNA	970:973	arg1	sequences					980:988	identical 16S rRNA gene sequences	956:988	identical 16S rRNA gene sequences	956:988	The two strains of both novel species shared identical 16S rRNA gene sequences but a MLSA allowed their intraspecies differentiation.
32996878	11	47	theme	strain	1861:1866	arg1	2.1615T					1872:1878	strain TMW 2.1615T	1861:1878	strain TMW 2.1615T	1861:1878	Peptidoglycan type of strain TMW 2.1615T is Lys-Ala and major fatty acids are C16 : 0, C19 : 0cyclo ω8c and summed feature 8.
32996878	3	48	theme	spoilage	180:187	arg1	microbiome					189:198	the spoilage microbiome	176:198	the spoilage microbiome of modified-atmosphere packaged beef from Germany	176:248	As part of a study investigating the spoilage microbiome of modified-atmosphere packaged beef from Germany, four novel strains of lactic acid bacteria were isolated and subsequently taxonomically characterized by a polyphasic approach, which revealed that they could not be assigned to known species.
32996878	11	49	theme	Peptidoglycan	1839:1851	arg1	type					1853:1856	Peptidoglycan type	1839:1856	Peptidoglycan type of strain TMW 2.1615T	1839:1878	Peptidoglycan type of strain TMW 2.1615T is Lys-Ala and major fatty acids are C16 : 0, C19 : 0cyclo ω8c and summed feature 8.
32996878	12	50	dep	sp	2115:2116	arg1	names					2088:2092	the names	2084:2092	the names	2084:2092	On the basis of polyphasic evidence, the meat isolates represent two novel species of the genus Lactococcus, for which the names Lactococcus carnosus sp.
32996878	10	51	theme	major	1787:1791	arg1	acids					1799:1803	major fatty acids	1787:1803	major fatty acids	1787:1803	The in silico DDH estimate value between the respective type strain TMW 2.1612T and TMW 2.1615T and the most closely related type strain of Lactococcus piscium was only 59.9 and 48.9 %, respectively, and 51.1 % between TMW 2.1612T and TMW 2.1615T. Peptidoglycan type of strain TMW 2.1612T is Lys-Thr-Ala and major fatty acids are summed feature 8 and C16 : 0.
32996878	10	51	theme	major	1787:1791	arg1	feature					1816:1822	summed feature 8	1809:1824	summed feature 8	1809:1824	The in silico DDH estimate value between the respective type strain TMW 2.1612T and TMW 2.1615T and the most closely related type strain of Lactococcus piscium was only 59.9 and 48.9 %, respectively, and 51.1 % between TMW 2.1612T and TMW 2.1615T. Peptidoglycan type of strain TMW 2.1612T is Lys-Thr-Ala and major fatty acids are summed feature 8 and C16 : 0.
32996878	10	52	theme	summed	1809:1814	arg1	acids					1799:1803	major fatty acids	1787:1803	major fatty acids	1787:1803	The in silico DDH estimate value between the respective type strain TMW 2.1612T and TMW 2.1615T and the most closely related type strain of Lactococcus piscium was only 59.9 and 48.9 %, respectively, and 51.1 % between TMW 2.1612T and TMW 2.1615T. Peptidoglycan type of strain TMW 2.1612T is Lys-Thr-Ala and major fatty acids are summed feature 8 and C16 : 0.
32996878	10	52	theme	summed	1809:1814	arg1	feature					1816:1822	summed feature 8	1809:1824	summed feature 8	1809:1824	The in silico DDH estimate value between the respective type strain TMW 2.1612T and TMW 2.1615T and the most closely related type strain of Lactococcus piscium was only 59.9 and 48.9 %, respectively, and 51.1 % between TMW 2.1612T and TMW 2.1615T. Peptidoglycan type of strain TMW 2.1612T is Lys-Thr-Ala and major fatty acids are summed feature 8 and C16 : 0.
32996878	6	53	theme	novel	812:816	arg1	species					818:824	two different novel species	798:824	two different novel species with two strains, (TMW 2.1612T/TMW 2.1613 and TMW 2.1615T/TMW 2.1614)	798:894	Results indicated that they represented two different novel species with two strains, (TMW 2.1612T/TMW 2.1613 and TMW 2.1615T/TMW 2.1614), respectively.
32996878	7	54	theme	16S	966:968	arg1	sequences					980:988	identical 16S rRNA gene sequences	956:988	identical 16S rRNA gene sequences	956:988	The two strains of both novel species shared identical 16S rRNA gene sequences but a MLSA allowed their intraspecies differentiation.
32996878	9	55	theme	Lactococcus	1371:1381	arg1	piscium					1383:1389	Lactococcus piscium	1371:1389	Lactococcus piscium	1371:1389	However, the ANIb value between the respective type strains TMW 2.1612T and TMW 2.1615T, and the type strain of Lactococcus piscium was only 94.3 and 92.0 %, respectively, and 92.9 % between TMW 2.1612T and TMW 2.1615T.
32996878	0	56	dep	sp	21:22	arg1	carnosus					12:19	Lactococcus carnosus	0:19	Lactococcus carnosus	0:19	Lactococcus carnosus sp.
32996878	6	57	with	species	818:824	arg1	strains					835:841	two strains	831:841	two strains, (TMW 2.1612T/TMW 2.1613 and TMW 2.1615T/TMW 2.1614)	831:894	Results indicated that they represented two different novel species with two strains, (TMW 2.1612T/TMW 2.1613 and TMW 2.1615T/TMW 2.1614), respectively.
32996878	7	58	theme	novel	935:939	arg1	species					941:947	both novel species	930:947	both novel species	930:947	The two strains of both novel species shared identical 16S rRNA gene sequences but a MLSA allowed their intraspecies differentiation.
32996878	2	59	attach	isolated	87:94	arg2	species					79:85	two novel species	69:85	two novel species isolated from modified-atmosphere	69:119	nov., two novel species isolated from modified-atmosphere packaged beef steaks.
32996878	2	59	attach	isolated	87:94	arg1	modified-atmosphere					101:119	modified-atmosphere	101:119	modified-atmosphere	101:119	nov., two novel species isolated from modified-atmosphere packaged beef steaks.
32996878	3	60	dep	isolated	299:306	arg1	characterized					339:351	characterized	339:351	characterized by a polyphasic approach, which revealed that they could not be assigned to known species	339:441	As part of a study investigating the spoilage microbiome of modified-atmosphere packaged beef from Germany, four novel strains of lactic acid bacteria were isolated and subsequently taxonomically characterized by a polyphasic approach, which revealed that they could not be assigned to known species.
32996878	5	61	theme	genus	666:670	arg1	Lactococcus					672:682	the genus Lactococcus	662:682	the genus Lactococcus	662:682	Morphological, physiological and phylogenetic analysis revealed a distinct lineage within the genus Lactococcus, with Lactococcus piscium and Lactococcus plantarum as closest relatives.
32996878	6	62	theme	2.1612T/TMW	849:859	arg1	2.1613					861:866	TMW 2.1612T/TMW 2.1613	845:866	TMW 2.1612T/TMW 2.1613	845:866	Results indicated that they represented two different novel species with two strains, (TMW 2.1612T/TMW 2.1613 and TMW 2.1615T/TMW 2.1614), respectively.
32996878	11	63	theme	fatty	1901:1905	arg1	acids					1907:1911	major fatty acids	1895:1911	major fatty acids	1895:1911	Peptidoglycan type of strain TMW 2.1615T is Lys-Ala and major fatty acids are C16 : 0, C19 : 0cyclo ω8c and summed feature 8.
32996878	8	64	theme	%	1182:1182	arg1	similarity					1110:1119	a similarity	1108:1119	a similarity of 99.85 % to each other	1108:1144	The 16S rRNA gene sequences of TMW 2.1612T and TMW 2.1615T had a similarity of 99.85 % to each other and a similarity of 99.85 and 99.78 % the most closely related type strain of Lactococcus piscium, respectively.
32996878	8	64	theme	%	1182:1182	arg1	similarity					1152:1161	a similarity	1150:1161	a similarity of 99.85 and 99.78 %	1150:1182	The 16S rRNA gene sequences of TMW 2.1612T and TMW 2.1615T had a similarity of 99.85 % to each other and a similarity of 99.85 and 99.78 % the most closely related type strain of Lactococcus piscium, respectively.
32996878	3	65	theme	novel	256:260	arg1	strains					262:268	four novel strains	251:268	four novel strains of lactic acid bacteria	251:292	As part of a study investigating the spoilage microbiome of modified-atmosphere packaged beef from Germany, four novel strains of lactic acid bacteria were isolated and subsequently taxonomically characterized by a polyphasic approach, which revealed that they could not be assigned to known species.
32996878	3	65	theme	novel	256:260	arg1	part					146:149	part	146:149	part of a study investigating the spoilage microbiome of modified-atmosphere packaged beef from Germany	146:248	As part of a study investigating the spoilage microbiome of modified-atmosphere packaged beef from Germany, four novel strains of lactic acid bacteria were isolated and subsequently taxonomically characterized by a polyphasic approach, which revealed that they could not be assigned to known species.
32996878	8	66	theme	rRNA	1053:1056	arg1	sequences					1063:1071	The 16S rRNA gene sequences	1045:1071	The 16S rRNA gene sequences of TMW 2.1612T and TMW 2.1615T	1045:1102	The 16S rRNA gene sequences of TMW 2.1612T and TMW 2.1615T had a similarity of 99.85 % to each other and a similarity of 99.85 and 99.78 % the most closely related type strain of Lactococcus piscium, respectively.
32996878	12	67	theme	polyphasic	1981:1990	arg1	evidence					1992:1999	polyphasic evidence	1981:1999	polyphasic evidence	1981:1999	On the basis of polyphasic evidence, the meat isolates represent two novel species of the genus Lactococcus, for which the names Lactococcus carnosus sp.
32996878	10	68	theme	TMW	1563:1565	arg1	2.1615T					1567:1573	TMW 2.1615T	1563:1573	TMW 2.1615T	1563:1573	The in silico DDH estimate value between the respective type strain TMW 2.1612T and TMW 2.1615T and the most closely related type strain of Lactococcus piscium was only 59.9 and 48.9 %, respectively, and 51.1 % between TMW 2.1612T and TMW 2.1615T. Peptidoglycan type of strain TMW 2.1612T is Lys-Thr-Ala and major fatty acids are summed feature 8 and C16 : 0.
32996878	10	69	theme	TMW	1714:1716	arg1	type					1741:1744	TMW 2.1612T and TMW 2.1615T. Peptidoglycan type	1698:1744	type	1741:1744	The in silico DDH estimate value between the respective type strain TMW 2.1612T and TMW 2.1615T and the most closely related type strain of Lactococcus piscium was only 59.9 and 48.9 %, respectively, and 51.1 % between TMW 2.1612T and TMW 2.1615T. Peptidoglycan type of strain TMW 2.1612T is Lys-Thr-Ala and major fatty acids are summed feature 8 and C16 : 0.
32996878	15	70	theme	TWM	2261:2263	arg1	30116T					2293:2298	=DSM 111017T =CECT 30116T	2274:2298	=DSM 111017T =CECT 30116T	2274:2298	The designated respective type strains are TMW 2.1612T (=DSM 111016T =CECT 30115T) and TWM 2.1615T (=DSM 111017T =CECT 30116T).
32996878	15	70	theme	TWM	2261:2263	arg1	2.1615T					2265:2271	TWM 2.1615T	2261:2271	TWM 2.1615T (=DSM 111017T =CECT 30116T)	2261:2299	The designated respective type strains are TMW 2.1612T (=DSM 111016T =CECT 30115T) and TWM 2.1615T (=DSM 111017T =CECT 30116T).
32996878	8	71	theme	related	1201:1207	arg1	strain					1214:1219	the most closely related type strain	1184:1219	the most closely related type strain of Lactococcus piscium	1184:1242	The 16S rRNA gene sequences of TMW 2.1612T and TMW 2.1615T had a similarity of 99.85 % to each other and a similarity of 99.85 and 99.78 % the most closely related type strain of Lactococcus piscium, respectively.
32996878	3	72	theme	acid	280:283	arg1	bacteria					285:292	lactic acid bacteria	273:292	lactic acid bacteria	273:292	As part of a study investigating the spoilage microbiome of modified-atmosphere packaged beef from Germany, four novel strains of lactic acid bacteria were isolated and subsequently taxonomically characterized by a polyphasic approach, which revealed that they could not be assigned to known species.
32996878	15	73	theme	111016T	2235:2241	arg1	30115T					2249:2254	=DSM 111016T =CECT 30115T	2230:2254	=DSM 111016T =CECT 30115T	2230:2254	The designated respective type strains are TMW 2.1612T (=DSM 111016T =CECT 30115T) and TWM 2.1615T (=DSM 111017T =CECT 30116T).
32996878	15	73	theme	111016T	2235:2241	arg1	2.1612T					2221:2227	TMW 2.1612T	2217:2227	TMW 2.1612T (=DSM 111016T =CECT 30115T)	2217:2255	The designated respective type strains are TMW 2.1612T (=DSM 111016T =CECT 30115T) and TWM 2.1615T (=DSM 111017T =CECT 30116T).
32996878	11	74	theme	TMW	1868:1870	arg1	2.1615T					1872:1878	strain TMW 2.1615T	1861:1878	strain TMW 2.1615T	1861:1878	Peptidoglycan type of strain TMW 2.1615T is Lys-Ala and major fatty acids are C16 : 0, C19 : 0cyclo ω8c and summed feature 8.
32996878	10	75	theme	Peptidoglycan	1727:1739	arg1	type					1741:1744	TMW 2.1612T and TMW 2.1615T. Peptidoglycan type	1698:1744	type	1741:1744	The in silico DDH estimate value between the respective type strain TMW 2.1612T and TMW 2.1615T and the most closely related type strain of Lactococcus piscium was only 59.9 and 48.9 %, respectively, and 51.1 % between TMW 2.1612T and TMW 2.1615T. Peptidoglycan type of strain TMW 2.1612T is Lys-Thr-Ala and major fatty acids are summed feature 8 and C16 : 0.
32996878	8	76	theme	Lactococcus	1224:1234	arg1	piscium					1236:1242	Lactococcus piscium	1224:1242	Lactococcus piscium	1224:1242	The 16S rRNA gene sequences of TMW 2.1612T and TMW 2.1615T had a similarity of 99.85 % to each other and a similarity of 99.85 and 99.78 % the most closely related type strain of Lactococcus piscium, respectively.
32996878	5	77	theme	closest	739:745	arg1	relatives					747:755	closest relatives	739:755	closest relatives	739:755	Morphological, physiological and phylogenetic analysis revealed a distinct lineage within the genus Lactococcus, with Lactococcus piscium and Lactococcus plantarum as closest relatives.
32996878	15	78	theme	TMW	2217:2219	arg1	strains					2205:2211	The designated respective type strains	2174:2211	The designated respective type strains	2174:2211	The designated respective type strains are TMW 2.1612T (=DSM 111016T =CECT 30115T) and TWM 2.1615T (=DSM 111017T =CECT 30116T).
32996878	15	78	theme	TMW	2217:2219	arg1	2.1612T					2221:2227	TMW 2.1612T	2217:2227	TMW 2.1612T (=DSM 111016T =CECT 30115T)	2217:2255	The designated respective type strains are TMW 2.1612T (=DSM 111016T =CECT 30115T) and TWM 2.1615T (=DSM 111017T =CECT 30116T).
32996878	15	78	theme	TMW	2217:2219	arg1	30115T					2249:2254	=DSM 111016T =CECT 30115T	2230:2254	=DSM 111016T =CECT 30115T	2230:2254	The designated respective type strains are TMW 2.1612T (=DSM 111016T =CECT 30115T) and TWM 2.1615T (=DSM 111017T =CECT 30116T).
32996878	9	79	theme	TMW	1466:1468	arg1	2.1615T					1470:1476	TMW 2.1615T	1466:1476	TMW 2.1615T	1466:1476	However, the ANIb value between the respective type strains TMW 2.1612T and TMW 2.1615T, and the type strain of Lactococcus piscium was only 94.3 and 92.0 %, respectively, and 92.9 % between TMW 2.1612T and TMW 2.1615T.
32996878	10	80	theme	TMW	1756:1758	arg1	2.1612T					1760:1766	strain TMW 2.1612T	1749:1766	strain TMW 2.1612T	1749:1766	The in silico DDH estimate value between the respective type strain TMW 2.1612T and TMW 2.1615T and the most closely related type strain of Lactococcus piscium was only 59.9 and 48.9 %, respectively, and 51.1 % between TMW 2.1612T and TMW 2.1615T. Peptidoglycan type of strain TMW 2.1612T is Lys-Thr-Ala and major fatty acids are summed feature 8 and C16 : 0.
32996878	12	81	dep	Lactococcus	2094:2104	arg1	carnosus					2106:2113	carnosus	2106:2113	carnosus	2106:2113	On the basis of polyphasic evidence, the meat isolates represent two novel species of the genus Lactococcus, for which the names Lactococcus carnosus sp.
32996878	15	82	theme	designated	2178:2187	arg1	strains					2205:2211	The designated respective type strains	2174:2211	The designated respective type strains	2174:2211	The designated respective type strains are TMW 2.1612T (=DSM 111016T =CECT 30115T) and TWM 2.1615T (=DSM 111017T =CECT 30116T).
32996878	15	82	theme	designated	2178:2187	arg1	2.1612T					2221:2227	TMW 2.1612T	2217:2227	TMW 2.1612T (=DSM 111016T =CECT 30115T)	2217:2255	The designated respective type strains are TMW 2.1612T (=DSM 111016T =CECT 30115T) and TWM 2.1615T (=DSM 111017T =CECT 30116T).
32996878	15	83	theme	=DSM	2274:2277	arg1	30116T					2293:2298	=DSM 111017T =CECT 30116T	2274:2298	=DSM 111017T =CECT 30116T	2274:2298	The designated respective type strains are TMW 2.1612T (=DSM 111016T =CECT 30115T) and TWM 2.1615T (=DSM 111017T =CECT 30116T).
32996878	15	83	theme	=DSM	2274:2277	arg1	2.1615T					2265:2271	TWM 2.1615T	2261:2271	TWM 2.1615T (=DSM 111017T =CECT 30116T)	2261:2299	The designated respective type strains are TMW 2.1612T (=DSM 111016T =CECT 30115T) and TWM 2.1615T (=DSM 111017T =CECT 30116T).
32996878	8	84	theme	TMW	1076:1078	arg1	2.1612T					1080:1086	TMW 2.1612T	1076:1086	TMW 2.1612T	1076:1086	The 16S rRNA gene sequences of TMW 2.1612T and TMW 2.1615T had a similarity of 99.85 % to each other and a similarity of 99.85 and 99.78 % the most closely related type strain of Lactococcus piscium, respectively.
32996878	15	85	theme	=CECT	2287:2291	arg1	30116T					2293:2298	=DSM 111017T =CECT 30116T	2274:2298	=DSM 111017T =CECT 30116T	2274:2298	The designated respective type strains are TMW 2.1612T (=DSM 111016T =CECT 30115T) and TWM 2.1615T (=DSM 111017T =CECT 30116T).
32996878	15	85	theme	=CECT	2287:2291	arg1	2.1615T					2265:2271	TWM 2.1615T	2261:2271	TWM 2.1615T (=DSM 111017T =CECT 30116T)	2261:2299	The designated respective type strains are TMW 2.1612T (=DSM 111016T =CECT 30115T) and TWM 2.1615T (=DSM 111017T =CECT 30116T).
32996878	3	86	theme	study	156:160	arg1	strains					262:268	four novel strains	251:268	four novel strains of lactic acid bacteria	251:292	As part of a study investigating the spoilage microbiome of modified-atmosphere packaged beef from Germany, four novel strains of lactic acid bacteria were isolated and subsequently taxonomically characterized by a polyphasic approach, which revealed that they could not be assigned to known species.
32996878	3	86	theme	study	156:160	arg1	part					146:149	part	146:149	part of a study investigating the spoilage microbiome of modified-atmosphere packaged beef from Germany	146:248	As part of a study investigating the spoilage microbiome of modified-atmosphere packaged beef from Germany, four novel strains of lactic acid bacteria were isolated and subsequently taxonomically characterized by a polyphasic approach, which revealed that they could not be assigned to known species.
32996878	9	87	theme	type	1306:1309	arg1	2.1612T					1323:1329	TMW 2.1612T	1319:1329	TMW 2.1612T	1319:1329	However, the ANIb value between the respective type strains TMW 2.1612T and TMW 2.1615T, and the type strain of Lactococcus piscium was only 94.3 and 92.0 %, respectively, and 92.9 % between TMW 2.1612T and TMW 2.1615T.
32996878	9	87	theme	type	1306:1309	arg1	2.1615T					1339:1345	TMW 2.1615T	1335:1345	TMW 2.1615T	1335:1345	However, the ANIb value between the respective type strains TMW 2.1612T and TMW 2.1615T, and the type strain of Lactococcus piscium was only 94.3 and 92.0 %, respectively, and 92.9 % between TMW 2.1612T and TMW 2.1615T.
32996878	9	87	theme	type	1306:1309	arg1	strains					1311:1317	the respective type strains	1291:1317	the respective type strains TMW 2.1612T and TMW 2.1615T	1291:1345	However, the ANIb value between the respective type strains TMW 2.1612T and TMW 2.1615T, and the type strain of Lactococcus piscium was only 94.3 and 92.0 %, respectively, and 92.9 % between TMW 2.1612T and TMW 2.1615T.
32996878	13	88	dep	sp	2152:2153	arg1	nov					2119:2121	nov	2119:2121	nov	2119:2121	nov and Lactococcus paracarnosus sp.
32996878	13	88	dep	sp	2152:2153	arg1	paracarnosus					2139:2150	Lactococcus paracarnosus	2127:2150	Lactococcus paracarnosus	2127:2150	nov and Lactococcus paracarnosus sp.
32996878	9	89	theme	TMW	1319:1321	arg1	2.1612T					1323:1329	TMW 2.1612T	1319:1329	TMW 2.1612T	1319:1329	However, the ANIb value between the respective type strains TMW 2.1612T and TMW 2.1615T, and the type strain of Lactococcus piscium was only 94.3 and 92.0 %, respectively, and 92.9 % between TMW 2.1612T and TMW 2.1615T.
32996878	9	89	theme	TMW	1319:1321	arg1	strains					1311:1317	the respective type strains	1291:1317	the respective type strains TMW 2.1612T and TMW 2.1615T	1291:1345	However, the ANIb value between the respective type strains TMW 2.1612T and TMW 2.1615T, and the type strain of Lactococcus piscium was only 94.3 and 92.0 %, respectively, and 92.9 % between TMW 2.1612T and TMW 2.1615T.
32996878	8	90	theme	2.1615T	1096:1102	arg1	sequences					1063:1071	The 16S rRNA gene sequences	1045:1071	The 16S rRNA gene sequences of TMW 2.1612T and TMW 2.1615T	1045:1102	The 16S rRNA gene sequences of TMW 2.1612T and TMW 2.1615T had a similarity of 99.85 % to each other and a similarity of 99.85 and 99.78 % the most closely related type strain of Lactococcus piscium, respectively.
32996878	3	91	theme	polyphasic	358:367	arg1	approach					369:376	a polyphasic approach	356:376	a polyphasic approach	356:376	As part of a study investigating the spoilage microbiome of modified-atmosphere packaged beef from Germany, four novel strains of lactic acid bacteria were isolated and subsequently taxonomically characterized by a polyphasic approach, which revealed that they could not be assigned to known species.
32996878	15	92	theme	respective	2189:2198	arg1	strains					2205:2211	The designated respective type strains	2174:2211	The designated respective type strains	2174:2211	The designated respective type strains are TMW 2.1612T (=DSM 111016T =CECT 30115T) and TWM 2.1615T (=DSM 111017T =CECT 30116T).
32996878	15	92	theme	respective	2189:2198	arg1	2.1612T					2221:2227	TMW 2.1612T	2217:2227	TMW 2.1612T (=DSM 111016T =CECT 30115T)	2217:2255	The designated respective type strains are TMW 2.1612T (=DSM 111016T =CECT 30115T) and TWM 2.1615T (=DSM 111017T =CECT 30116T).
32996878	10	93	theme	type	1604:1607	arg1	strain					1609:1614	the most closely related type strain	1579:1614	the most closely related type strain of Lactococcus piscium	1579:1637	The in silico DDH estimate value between the respective type strain TMW 2.1612T and TMW 2.1615T and the most closely related type strain of Lactococcus piscium was only 59.9 and 48.9 %, respectively, and 51.1 % between TMW 2.1612T and TMW 2.1615T. Peptidoglycan type of strain TMW 2.1612T is Lys-Thr-Ala and major fatty acids are summed feature 8 and C16 : 0.
32996878	12	94	theme	genus	2055:2059	arg1	Lactococcus					2061:2071	the genus Lactococcus	2051:2071	the genus Lactococcus	2051:2071	On the basis of polyphasic evidence, the meat isolates represent two novel species of the genus Lactococcus, for which the names Lactococcus carnosus sp.
32996878	3	95	theme	known	429:433	arg1	species					435:441	known species	429:441	known species	429:441	As part of a study investigating the spoilage microbiome of modified-atmosphere packaged beef from Germany, four novel strains of lactic acid bacteria were isolated and subsequently taxonomically characterized by a polyphasic approach, which revealed that they could not be assigned to known species.
32996878	12	96	dep	evidence	1992:1999	arg1	basis					1972:1976	basis	1972:1976	basis	1972:1976	On the basis of polyphasic evidence, the meat isolates represent two novel species of the genus Lactococcus, for which the names Lactococcus carnosus sp.
32996878	12	96	dep	evidence	1992:1999	arg1	the					1968:1970	the	1968:1970	the	1968:1970	On the basis of polyphasic evidence, the meat isolates represent two novel species of the genus Lactococcus, for which the names Lactococcus carnosus sp.
32996878	7	97	theme	gene	975:978	arg1	sequences					980:988	identical 16S rRNA gene sequences	956:988	identical 16S rRNA gene sequences	956:988	The two strains of both novel species shared identical 16S rRNA gene sequences but a MLSA allowed their intraspecies differentiation.
32996878	3	98	from	Germany	242:248	arg1	beef					232:235	beef	232:235	beef	232:235	As part of a study investigating the spoilage microbiome of modified-atmosphere packaged beef from Germany, four novel strains of lactic acid bacteria were isolated and subsequently taxonomically characterized by a polyphasic approach, which revealed that they could not be assigned to known species.
32996878	10	99	theme	fatty	1793:1797	arg1	acids					1799:1803	major fatty acids	1787:1803	major fatty acids	1787:1803	The in silico DDH estimate value between the respective type strain TMW 2.1612T and TMW 2.1615T and the most closely related type strain of Lactococcus piscium was only 59.9 and 48.9 %, respectively, and 51.1 % between TMW 2.1612T and TMW 2.1615T. Peptidoglycan type of strain TMW 2.1612T is Lys-Thr-Ala and major fatty acids are summed feature 8 and C16 : 0.
32996878	10	99	theme	fatty	1793:1797	arg1	feature					1816:1822	summed feature 8	1809:1824	summed feature 8	1809:1824	The in silico DDH estimate value between the respective type strain TMW 2.1612T and TMW 2.1615T and the most closely related type strain of Lactococcus piscium was only 59.9 and 48.9 %, respectively, and 51.1 % between TMW 2.1612T and TMW 2.1615T. Peptidoglycan type of strain TMW 2.1612T is Lys-Thr-Ala and major fatty acids are summed feature 8 and C16 : 0.
32996878	10	100	theme	piscium	1631:1637	arg1	strain					1609:1614	the most closely related type strain	1579:1614	the most closely related type strain of Lactococcus piscium	1579:1637	The in silico DDH estimate value between the respective type strain TMW 2.1612T and TMW 2.1615T and the most closely related type strain of Lactococcus piscium was only 59.9 and 48.9 %, respectively, and 51.1 % between TMW 2.1612T and TMW 2.1615T. Peptidoglycan type of strain TMW 2.1612T is Lys-Thr-Ala and major fatty acids are summed feature 8 and C16 : 0.
32996878	10	100	theme	piscium	1631:1637	arg1	strain					1540:1545	the respective type strain	1520:1545	the respective type strain TMW 2.1612T and TMW 2.1615T	1520:1573	The in silico DDH estimate value between the respective type strain TMW 2.1612T and TMW 2.1615T and the most closely related type strain of Lactococcus piscium was only 59.9 and 48.9 %, respectively, and 51.1 % between TMW 2.1612T and TMW 2.1615T. Peptidoglycan type of strain TMW 2.1612T is Lys-Thr-Ala and major fatty acids are summed feature 8 and C16 : 0.
32996878	8	101	theme	%	1130:1130	arg1	similarity					1110:1119	a similarity	1108:1119	a similarity of 99.85 % to each other	1108:1144	The 16S rRNA gene sequences of TMW 2.1612T and TMW 2.1615T had a similarity of 99.85 % to each other and a similarity of 99.85 and 99.78 % the most closely related type strain of Lactococcus piscium, respectively.
32996878	8	101	theme	%	1130:1130	arg1	similarity					1152:1161	a similarity	1150:1161	a similarity of 99.85 and 99.78 %	1150:1182	The 16S rRNA gene sequences of TMW 2.1612T and TMW 2.1615T had a similarity of 99.85 % to each other and a similarity of 99.85 and 99.78 % the most closely related type strain of Lactococcus piscium, respectively.
32996878	12	102	theme	novel	2034:2038	arg1	species					2040:2046	two novel species	2030:2046	two novel species	2030:2046	On the basis of polyphasic evidence, the meat isolates represent two novel species of the genus Lactococcus, for which the names Lactococcus carnosus sp.
32996878	9	103	theme	type	1356:1359	arg1	strain					1361:1366	the type strain	1352:1366	the type strain of Lactococcus piscium	1352:1389	However, the ANIb value between the respective type strains TMW 2.1612T and TMW 2.1615T, and the type strain of Lactococcus piscium was only 94.3 and 92.0 %, respectively, and 92.9 % between TMW 2.1612T and TMW 2.1615T.
32996878	9	104	dep	strains	1311:1317	arg1	2.1612T					1323:1329	TMW 2.1612T	1319:1329	TMW 2.1612T	1319:1329	However, the ANIb value between the respective type strains TMW 2.1612T and TMW 2.1615T, and the type strain of Lactococcus piscium was only 94.3 and 92.0 %, respectively, and 92.9 % between TMW 2.1612T and TMW 2.1615T.
32996878	9	104	dep	strains	1311:1317	arg1	2.1615T					1339:1345	TMW 2.1615T	1335:1345	TMW 2.1615T	1335:1345	However, the ANIb value between the respective type strains TMW 2.1612T and TMW 2.1615T, and the type strain of Lactococcus piscium was only 94.3 and 92.0 %, respectively, and 92.9 % between TMW 2.1612T and TMW 2.1615T.
32996878	9	104	dep	strains	1311:1317	arg1	strains					1311:1317	the respective type strains	1291:1317	the respective type strains TMW 2.1612T and TMW 2.1615T	1291:1345	However, the ANIb value between the respective type strains TMW 2.1612T and TMW 2.1615T, and the type strain of Lactococcus piscium was only 94.3 and 92.0 %, respectively, and 92.9 % between TMW 2.1612T and TMW 2.1615T.
32996878	10	105	theme	in	1483:1484	arg1	value					1506:1510	The in silico DDH estimate value	1479:1510	The in silico DDH estimate value between the respective type strain TMW 2.1612T and TMW 2.1615T and the most closely related type strain of Lactococcus piscium	1479:1637	The in silico DDH estimate value between the respective type strain TMW 2.1612T and TMW 2.1615T and the most closely related type strain of Lactococcus piscium was only 59.9 and 48.9 %, respectively, and 51.1 % between TMW 2.1612T and TMW 2.1615T. Peptidoglycan type of strain TMW 2.1612T is Lys-Thr-Ala and major fatty acids are summed feature 8 and C16 : 0.
32996878	10	105	theme	in	1483:1484	arg1	59.9					1648:1651	59.9	1648:1651	59.9	1648:1651	The in silico DDH estimate value between the respective type strain TMW 2.1612T and TMW 2.1615T and the most closely related type strain of Lactococcus piscium was only 59.9 and 48.9 %, respectively, and 51.1 % between TMW 2.1612T and TMW 2.1615T. Peptidoglycan type of strain TMW 2.1612T is Lys-Thr-Ala and major fatty acids are summed feature 8 and C16 : 0.
32996878	7	106	theme	identical	956:964	arg1	sequences					980:988	identical 16S rRNA gene sequences	956:988	identical 16S rRNA gene sequences	956:988	The two strains of both novel species shared identical 16S rRNA gene sequences but a MLSA allowed their intraspecies differentiation.
32996878	1	107	theme	nov.	25:28	arg1	paracarnosus					46:57	nov. and Lactococcus paracarnosus	25:57	nov. and Lactococcus paracarnosus	25:57	nov. and Lactococcus paracarnosus sp.
32996878	12	108	theme	meat	2006:2009	arg1	isolates					2011:2018	the meat isolates	2002:2018	the meat isolates	2002:2018	On the basis of polyphasic evidence, the meat isolates represent two novel species of the genus Lactococcus, for which the names Lactococcus carnosus sp.
32996878	6	109	theme	different	802:810	arg1	species					818:824	two different novel species	798:824	two different novel species with two strains, (TMW 2.1612T/TMW 2.1613 and TMW 2.1615T/TMW 2.1614)	798:894	Results indicated that they represented two different novel species with two strains, (TMW 2.1612T/TMW 2.1613 and TMW 2.1615T/TMW 2.1614), respectively.
32996878	7	110	theme	species	941:947	arg1	strains					919:925	The two strains	911:925	The two strains of both novel species	911:947	The two strains of both novel species shared identical 16S rRNA gene sequences but a MLSA allowed their intraspecies differentiation.
32996878	9	111	theme	piscium	1383:1389	arg1	2.1612T					1323:1329	TMW 2.1612T	1319:1329	TMW 2.1612T	1319:1329	However, the ANIb value between the respective type strains TMW 2.1612T and TMW 2.1615T, and the type strain of Lactococcus piscium was only 94.3 and 92.0 %, respectively, and 92.9 % between TMW 2.1612T and TMW 2.1615T.
32996878	9	111	theme	piscium	1383:1389	arg1	strain					1361:1366	the type strain	1352:1366	the type strain of Lactococcus piscium	1352:1389	However, the ANIb value between the respective type strains TMW 2.1612T and TMW 2.1615T, and the type strain of Lactococcus piscium was only 94.3 and 92.0 %, respectively, and 92.9 % between TMW 2.1612T and TMW 2.1615T.
32996878	9	111	theme	piscium	1383:1389	arg1	2.1615T					1339:1345	TMW 2.1615T	1335:1345	TMW 2.1615T	1335:1345	However, the ANIb value between the respective type strains TMW 2.1612T and TMW 2.1615T, and the type strain of Lactococcus piscium was only 94.3 and 92.0 %, respectively, and 92.9 % between TMW 2.1612T and TMW 2.1615T.
32996878	9	111	theme	piscium	1383:1389	arg1	strains					1311:1317	the respective type strains	1291:1317	the respective type strains TMW 2.1612T and TMW 2.1615T	1291:1345	However, the ANIb value between the respective type strains TMW 2.1612T and TMW 2.1615T, and the type strain of Lactococcus piscium was only 94.3 and 92.0 %, respectively, and 92.9 % between TMW 2.1612T and TMW 2.1615T.
32996878	6	112	theme	2.1615T/TMW	876:886	arg1	2.1614					888:893	TMW 2.1615T/TMW 2.1614	872:893	TMW 2.1615T/TMW 2.1614	872:893	Results indicated that they represented two different novel species with two strains, (TMW 2.1612T/TMW 2.1613 and TMW 2.1615T/TMW 2.1614), respectively.
32996878	1	113	theme	Lactococcus	34:44	arg1	paracarnosus					46:57	nov. and Lactococcus paracarnosus	25:57	nov. and Lactococcus paracarnosus	25:57	nov. and Lactococcus paracarnosus sp.
32996878	2	114	theme	beef	130:133	arg1	steaks					135:140	beef steaks	130:140	beef steaks	130:140	nov., two novel species isolated from modified-atmosphere packaged beef steaks.
32833615	4	0	theme	<1 	480:482	arg1	%					483:483	%	483:483	%	483:483	Strain J5903T grew optimally at 20‒25 °C, at pH 7.0‒7.5 and with <1 % (w/v) NaCl.
32833615	9	1	dep	anteiso-C15 	860:871	arg1	 0					873:874	 0	873:874	 0	873:874	The major whole-cell fatty acids were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
32833615	9	1	dep	anteiso-C15 	860:871	arg1	 0					906:907	 0	906:907	 0	906:907	The major whole-cell fatty acids were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
32833615	9	1	dep	anteiso-C15 	860:871	arg1	anteiso-C17 					893:904	anteiso-C17 	893:904	anteiso-C17 	893:904	The major whole-cell fatty acids were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
32833615	9	1	dep	anteiso-C15 	860:871	arg1	 0					886:887	 0	886:887	 0	886:887	The major whole-cell fatty acids were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
32833615	12	2	theme	bootstrap	1199:1207	arg1	support					1209:1215	high bootstrap support	1194:1215	high bootstrap support	1194:1215	Phylogenies based on genome sequence showed that strain J5903T and P. deserti 13S1-3T formed a robust cluster with high bootstrap support.
32833615	13	3	theme	P.	1283:1284	arg1	13S1-3T					1294:1300	P. deserti 13S1-3T	1283:1300	P. deserti 13S1-3T	1283:1300	Strain J5903T shared typical chemotaxonomic characteristics with P. deserti 13S1-3T.
32833615	3	4	theme	Gram-stain-positive	345:363	arg1	cocci					408:412	Gram-stain-positive, aerobic, non-motile and non-spore-forming cocci	345:412	Gram-stain-positive, aerobic, non-motile and non-spore-forming cocci	345:412	Cells of the isolate were Gram-stain-positive, aerobic, non-motile and non-spore-forming cocci.
32833615	5	5	theme	l-2,4-diaminobutyric	573:592	arg1	acid					594:597	l-2,4-diaminobutyric acid	573:597	l-2,4-diaminobutyric acid	573:597	The cell-wall peptidoglycan type was B2γ with d-2,4-diaminobutyric acid and l-2,4-diaminobutyric acid as diagnostic amino acids.
32833615	2	6	theme	Hebei	293:297	arg1	Province					299:306	Hebei Province	293:306	Hebei Province	293:306	A psychrotolerant actinobacterium, designated strain J5903T, was isolated from an alkaline soil sample from the rhizosphere of Suaeda salsa collected in desertification land surrounding Jiuliancheng Nur in Hebei Province, PR China.
32833615	2	6	theme	Hebei	293:297	arg1	China					312:316	PR China	309:316	PR China	309:316	A psychrotolerant actinobacterium, designated strain J5903T, was isolated from an alkaline soil sample from the rhizosphere of Suaeda salsa collected in desertification land surrounding Jiuliancheng Nur in Hebei Province, PR China.
32833615	16	7	theme	type	1490:1493	arg1	J5903T					1505:1510	J5903T	1505:1510	J5903T (=DSM 101894T=CGMCC 1.15523T)	1505:1540	The type strain is J5903T (=DSM 101894T=CGMCC 1.15523T).
32833615	16	7	theme	type	1490:1493	arg1	strain					1495:1500	The type strain	1486:1500	The type strain	1486:1500	The type strain is J5903T (=DSM 101894T=CGMCC 1.15523T).
32833615	10	8	theme	DNA	922:924	arg1	content					930:936	The genomic DNA G+C content	910:936	The genomic DNA G+C content	910:936	The genomic DNA G+C content was 69.1 mol%.
32833615	10	8	theme	DNA	922:924	arg1	%					950:950	69.1 mol%	942:950	69.1 mol%	942:950	The genomic DNA G+C content was 69.1 mol%.
32833615	4	9	theme	Strain	415:420	arg1	J5903T					422:427	Strain J5903T	415:427	Strain J5903T	415:427	Strain J5903T grew optimally at 20‒25 °C, at pH 7.0‒7.5 and with <1 % (w/v) NaCl.
32833615	9	10	theme	major	826:830	arg1	anteiso-C15 					860:871	anteiso-C15 	860:871	anteiso-C15 	860:871	The major whole-cell fatty acids were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
32833615	9	10	theme	major	826:830	arg1	acids					849:853	The major whole-cell fatty acids	822:853	The major whole-cell fatty acids	822:853	The major whole-cell fatty acids were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
32833615	3	11	theme	isolate	332:338	arg1	Cells					319:323	Cells	319:323	Cells of the isolate	319:338	Cells of the isolate were Gram-stain-positive, aerobic, non-motile and non-spore-forming cocci.
32833615	5	12	theme	diagnostic	602:611	arg1	acids					619:623	diagnostic amino acids	602:623	diagnostic amino acids	602:623	The cell-wall peptidoglycan type was B2γ with d-2,4-diaminobutyric acid and l-2,4-diaminobutyric acid as diagnostic amino acids.
32833615	5	12	theme	diagnostic	602:611	arg1	type					525:528	The cell-wall peptidoglycan type	497:528	The cell-wall peptidoglycan type	497:528	The cell-wall peptidoglycan type was B2γ with d-2,4-diaminobutyric acid and l-2,4-diaminobutyric acid as diagnostic amino acids.
32833615	5	13	theme	amino	613:617	arg1	acids					619:623	diagnostic amino acids	602:623	diagnostic amino acids	602:623	The cell-wall peptidoglycan type was B2γ with d-2,4-diaminobutyric acid and l-2,4-diaminobutyric acid as diagnostic amino acids.
32833615	5	13	theme	amino	613:617	arg1	type					525:528	The cell-wall peptidoglycan type	497:528	The cell-wall peptidoglycan type	497:528	The cell-wall peptidoglycan type was B2γ with d-2,4-diaminobutyric acid and l-2,4-diaminobutyric acid as diagnostic amino acids.
32833615	1	14	theme	Suaeda	73:78	arg1	salsa					80:84	Suaeda salsa	73:84	Suaeda salsa	73:84	nov., isolated from rhizosphere soil of Suaeda salsa.
32833615	8	15	theme	major	720:724	arg1	diphosphatidylglycerol					744:765	diphosphatidylglycerol	744:765	diphosphatidylglycerol	744:765	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and one unidentified glycolipid.
32833615	8	15	theme	major	720:724	arg1	lipids					732:737	The major polar lipids	716:737	The major polar lipids	716:737	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and one unidentified glycolipid.
32833615	3	16	theme	non-motile	375:384	arg1	cocci					408:412	Gram-stain-positive, aerobic, non-motile and non-spore-forming cocci	345:412	Gram-stain-positive, aerobic, non-motile and non-spore-forming cocci	345:412	Cells of the isolate were Gram-stain-positive, aerobic, non-motile and non-spore-forming cocci.
32833615	1	17	theme	rhizosphere	53:63	arg1	soil					65:68	rhizosphere soil	53:68	rhizosphere soil of Suaeda salsa	53:84	nov., isolated from rhizosphere soil of Suaeda salsa.
32833615	9	18	theme	fatty	843:847	arg1	anteiso-C15 					860:871	anteiso-C15 	860:871	anteiso-C15 	860:871	The major whole-cell fatty acids were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
32833615	9	18	theme	fatty	843:847	arg1	acids					849:853	The major whole-cell fatty acids	822:853	The major whole-cell fatty acids	822:853	The major whole-cell fatty acids were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
32833615	16	19	theme	101894T=CGMCC	1518:1530	arg1	1.15523T					1532:1539	=DSM 101894T=CGMCC 1.15523T	1513:1539	=DSM 101894T=CGMCC 1.15523T	1513:1539	The type strain is J5903T (=DSM 101894T=CGMCC 1.15523T).
32833615	16	19	theme	101894T=CGMCC	1518:1530	arg1	J5903T					1505:1510	J5903T	1505:1510	J5903T (=DSM 101894T=CGMCC 1.15523T)	1505:1540	The type strain is J5903T (=DSM 101894T=CGMCC 1.15523T).
32833615	1	20	theme	salsa	80:84	arg1	soil					65:68	rhizosphere soil	53:68	rhizosphere soil of Suaeda salsa	53:84	nov., isolated from rhizosphere soil of Suaeda salsa.
32833615	14	21	theme	name	1430:1433	arg1	Planctomonas					1435:1446	the name Planctomonas	1426:1446	the name Planctomonas	1426:1446	Combining the polyphasic taxonomic evidence, strain J5903T represents a novel species of the genus Planctomonas, for which the name Planctomonas psychrotolerans sp.
32833615	11	22	theme	hybridization	1023:1035	arg1	values					1037:1042	digital DNA-DNA hybridization values	1007:1042	digital DNA-DNA hybridization values	1007:1042	It shared the highest average nucleotide identity and digital DNA-DNA hybridization values with Planctomonas deserti 13S1-3T.
32833615	14	23	theme	strain	1348:1353	arg1	J5903T					1355:1360	strain J5903T	1348:1360	strain J5903T	1348:1360	Combining the polyphasic taxonomic evidence, strain J5903T represents a novel species of the genus Planctomonas, for which the name Planctomonas psychrotolerans sp.
32833615	5	24	theme	d-2,4-diaminobutyric	543:562	arg1	acid					564:567	d-2,4-diaminobutyric acid	543:567	d-2,4-diaminobutyric acid	543:567	The cell-wall peptidoglycan type was B2γ with d-2,4-diaminobutyric acid and l-2,4-diaminobutyric acid as diagnostic amino acids.
32833615	2	25	theme	PR	309:310	arg1	Province					299:306	Hebei Province	293:306	Hebei Province	293:306	A psychrotolerant actinobacterium, designated strain J5903T, was isolated from an alkaline soil sample from the rhizosphere of Suaeda salsa collected in desertification land surrounding Jiuliancheng Nur in Hebei Province, PR China.
32833615	2	25	theme	PR	309:310	arg1	China					312:316	PR China	309:316	PR China	309:316	A psychrotolerant actinobacterium, designated strain J5903T, was isolated from an alkaline soil sample from the rhizosphere of Suaeda salsa collected in desertification land surrounding Jiuliancheng Nur in Hebei Province, PR China.
32833615	13	26	theme	deserti	1286:1292	arg1	13S1-3T					1294:1300	P. deserti 13S1-3T	1283:1300	P. deserti 13S1-3T	1283:1300	Strain J5903T shared typical chemotaxonomic characteristics with P. deserti 13S1-3T.
32833615	3	27	theme	aerobic	366:372	arg1	cocci					408:412	Gram-stain-positive, aerobic, non-motile and non-spore-forming cocci	345:412	Gram-stain-positive, aerobic, non-motile and non-spore-forming cocci	345:412	Cells of the isolate were Gram-stain-positive, aerobic, non-motile and non-spore-forming cocci.
32833615	10	28	theme	G+C	926:928	arg1	content					930:936	The genomic DNA G+C content	910:936	The genomic DNA G+C content	910:936	The genomic DNA G+C content was 69.1 mol%.
32833615	10	28	theme	G+C	926:928	arg1	%					950:950	69.1 mol%	942:950	69.1 mol%	942:950	The genomic DNA G+C content was 69.1 mol%.
32833615	10	29	theme	genomic	914:920	arg1	content					930:936	The genomic DNA G+C content	910:936	The genomic DNA G+C content	910:936	The genomic DNA G+C content was 69.1 mol%.
32833615	10	29	theme	genomic	914:920	arg1	%					950:950	69.1 mol%	942:950	69.1 mol%	942:950	The genomic DNA G+C content was 69.1 mol%.
32833615	2	30	theme	soil	178:181	arg1	sample					183:188	an alkaline soil sample	166:188	an alkaline soil sample	166:188	A psychrotolerant actinobacterium, designated strain J5903T, was isolated from an alkaline soil sample from the rhizosphere of Suaeda salsa collected in desertification land surrounding Jiuliancheng Nur in Hebei Province, PR China.
32833615	16	31	theme	=DSM	1513:1516	arg1	1.15523T					1532:1539	=DSM 101894T=CGMCC 1.15523T	1513:1539	=DSM 101894T=CGMCC 1.15523T	1513:1539	The type strain is J5903T (=DSM 101894T=CGMCC 1.15523T).
32833615	16	31	theme	=DSM	1513:1516	arg1	J5903T					1505:1510	J5903T	1505:1510	J5903T (=DSM 101894T=CGMCC 1.15523T)	1505:1540	The type strain is J5903T (=DSM 101894T=CGMCC 1.15523T).
32833615	12	32	theme	high	1194:1197	arg1	support					1209:1215	high bootstrap support	1194:1215	high bootstrap support	1194:1215	Phylogenies based on genome sequence showed that strain J5903T and P. deserti 13S1-3T formed a robust cluster with high bootstrap support.
32833615	14	33	theme	taxonomic	1328:1336	arg1	evidence					1338:1345	the polyphasic taxonomic evidence	1313:1345	the polyphasic taxonomic evidence	1313:1345	Combining the polyphasic taxonomic evidence, strain J5903T represents a novel species of the genus Planctomonas, for which the name Planctomonas psychrotolerans sp.
32833615	11	34	theme	nucleotide	983:992	arg1	identity					994:1001	average nucleotide identity	975:1001	average nucleotide identity	975:1001	It shared the highest average nucleotide identity and digital DNA-DNA hybridization values with Planctomonas deserti 13S1-3T.
32833615	2	35	theme	alkaline	169:176	arg1	sample					183:188	an alkaline soil sample	166:188	an alkaline soil sample	166:188	A psychrotolerant actinobacterium, designated strain J5903T, was isolated from an alkaline soil sample from the rhizosphere of Suaeda salsa collected in desertification land surrounding Jiuliancheng Nur in Hebei Province, PR China.
32833615	10	36	theme	69.1 mol	942:949	arg1	content					930:936	The genomic DNA G+C content	910:936	The genomic DNA G+C content	910:936	The genomic DNA G+C content was 69.1 mol%.
32833615	10	36	theme	69.1 mol	942:949	arg1	%					950:950	69.1 mol%	942:950	69.1 mol%	942:950	The genomic DNA G+C content was 69.1 mol%.
32833615	6	37	theme	acetyl	650:655	arg1	residue					638:644	The muramyl residue	626:644	The muramyl residue	626:644	The muramyl residue was acetyl type.
32833615	6	37	theme	acetyl	650:655	arg1	type					657:660	acetyl type	650:660	acetyl type	650:660	The muramyl residue was acetyl type.
32833615	11	38	theme	deserti	1062:1068	arg1	13S1-3T					1070:1076	Planctomonas deserti 13S1-3T	1049:1076	Planctomonas deserti 13S1-3T	1049:1076	It shared the highest average nucleotide identity and digital DNA-DNA hybridization values with Planctomonas deserti 13S1-3T.
32833615	14	39	theme	polyphasic	1317:1326	arg1	evidence					1338:1345	the polyphasic taxonomic evidence	1313:1345	the polyphasic taxonomic evidence	1313:1345	Combining the polyphasic taxonomic evidence, strain J5903T represents a novel species of the genus Planctomonas, for which the name Planctomonas psychrotolerans sp.
32833615	2	40	theme	psychrotolerant	89:103	arg1	actinobacterium					105:119	A psychrotolerant actinobacterium	87:119	A psychrotolerant actinobacterium	87:119	A psychrotolerant actinobacterium, designated strain J5903T, was isolated from an alkaline soil sample from the rhizosphere of Suaeda salsa collected in desertification land surrounding Jiuliancheng Nur in Hebei Province, PR China.
32833615	2	41	theme	salsa	221:225	arg1	rhizosphere					199:209	the rhizosphere	195:209	the rhizosphere of Suaeda salsa collected in desertification land surrounding Jiuliancheng Nur in Hebei Province, PR China	195:316	A psychrotolerant actinobacterium, designated strain J5903T, was isolated from an alkaline soil sample from the rhizosphere of Suaeda salsa collected in desertification land surrounding Jiuliancheng Nur in Hebei Province, PR China.
32833615	5	42	theme	cell-wall	501:509	arg1	acids					619:623	diagnostic amino acids	602:623	diagnostic amino acids	602:623	The cell-wall peptidoglycan type was B2γ with d-2,4-diaminobutyric acid and l-2,4-diaminobutyric acid as diagnostic amino acids.
32833615	5	42	theme	cell-wall	501:509	arg1	type					525:528	The cell-wall peptidoglycan type	497:528	The cell-wall peptidoglycan type	497:528	The cell-wall peptidoglycan type was B2γ with d-2,4-diaminobutyric acid and l-2,4-diaminobutyric acid as diagnostic amino acids.
32833615	12	43	theme	robust	1174:1179	arg1	cluster					1181:1187	a robust cluster	1172:1187	a robust cluster	1172:1187	Phylogenies based on genome sequence showed that strain J5903T and P. deserti 13S1-3T formed a robust cluster with high bootstrap support.
32833615	3	44	theme	non-spore-forming	390:406	arg1	cocci					408:412	Gram-stain-positive, aerobic, non-motile and non-spore-forming cocci	345:412	Gram-stain-positive, aerobic, non-motile and non-spore-forming cocci	345:412	Cells of the isolate were Gram-stain-positive, aerobic, non-motile and non-spore-forming cocci.
32833615	5	45	theme	peptidoglycan	511:523	arg1	acids					619:623	diagnostic amino acids	602:623	diagnostic amino acids	602:623	The cell-wall peptidoglycan type was B2γ with d-2,4-diaminobutyric acid and l-2,4-diaminobutyric acid as diagnostic amino acids.
32833615	5	45	theme	peptidoglycan	511:523	arg1	type					525:528	The cell-wall peptidoglycan type	497:528	The cell-wall peptidoglycan type	497:528	The cell-wall peptidoglycan type was B2γ with d-2,4-diaminobutyric acid and l-2,4-diaminobutyric acid as diagnostic amino acids.
32833615	11	46	theme	average	975:981	arg1	identity					994:1001	average nucleotide identity	975:1001	average nucleotide identity	975:1001	It shared the highest average nucleotide identity and digital DNA-DNA hybridization values with Planctomonas deserti 13S1-3T.
32833615	12	47	theme	genome	1100:1105	arg1	sequence					1107:1114	genome sequence	1100:1114	genome sequence	1100:1114	Phylogenies based on genome sequence showed that strain J5903T and P. deserti 13S1-3T formed a robust cluster with high bootstrap support.
32833615	4	48	dep	%	483:483	arg1	w/v					486:488	w/v	486:488	w/v	486:488	Strain J5903T grew optimally at 20‒25 °C, at pH 7.0‒7.5 and with <1 % (w/v) NaCl.
32833615	4	49	theme	%	483:483	arg1	NaCl					491:494	<1 % (w/v) NaCl	480:494	<1 % (w/v) NaCl	480:494	Strain J5903T grew optimally at 20‒25 °C, at pH 7.0‒7.5 and with <1 % (w/v) NaCl.
32833615	13	50	theme	typical	1239:1245	arg1	characteristics					1262:1276	typical chemotaxonomic characteristics	1239:1276	typical chemotaxonomic characteristics	1239:1276	Strain J5903T shared typical chemotaxonomic characteristics with P. deserti 13S1-3T.
32833615	9	51	theme	whole-cell	832:841	arg1	anteiso-C15 					860:871	anteiso-C15 	860:871	anteiso-C15 	860:871	The major whole-cell fatty acids were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
32833615	9	51	theme	whole-cell	832:841	arg1	acids					849:853	The major whole-cell fatty acids	822:853	The major whole-cell fatty acids	822:853	The major whole-cell fatty acids were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0.
32833615	2	52	attach	isolated	152:159	arg2	actinobacterium					105:119	A psychrotolerant actinobacterium	87:119	A psychrotolerant actinobacterium	87:119	A psychrotolerant actinobacterium, designated strain J5903T, was isolated from an alkaline soil sample from the rhizosphere of Suaeda salsa collected in desertification land surrounding Jiuliancheng Nur in Hebei Province, PR China.
32833615	2	52	attach	isolated	152:159	arg1	sample					183:188	an alkaline soil sample	166:188	an alkaline soil sample	166:188	A psychrotolerant actinobacterium, designated strain J5903T, was isolated from an alkaline soil sample from the rhizosphere of Suaeda salsa collected in desertification land surrounding Jiuliancheng Nur in Hebei Province, PR China.
32833615	2	52	attach	isolated	152:159	arg1	rhizosphere					199:209	the rhizosphere	195:209	the rhizosphere of Suaeda salsa collected in desertification land surrounding Jiuliancheng Nur in Hebei Province, PR China	195:316	A psychrotolerant actinobacterium, designated strain J5903T, was isolated from an alkaline soil sample from the rhizosphere of Suaeda salsa collected in desertification land surrounding Jiuliancheng Nur in Hebei Province, PR China.
32833615	2	53	from	Nur	286:288	arg1	Province					299:306	Hebei Province	293:306	Hebei Province	293:306	A psychrotolerant actinobacterium, designated strain J5903T, was isolated from an alkaline soil sample from the rhizosphere of Suaeda salsa collected in desertification land surrounding Jiuliancheng Nur in Hebei Province, PR China.
32833615	2	53	from	Nur	286:288	arg1	China					312:316	PR China	309:316	PR China	309:316	A psychrotolerant actinobacterium, designated strain J5903T, was isolated from an alkaline soil sample from the rhizosphere of Suaeda salsa collected in desertification land surrounding Jiuliancheng Nur in Hebei Province, PR China.
32833615	13	54	theme	chemotaxonomic	1247:1260	arg1	characteristics					1262:1276	typical chemotaxonomic characteristics	1239:1276	typical chemotaxonomic characteristics	1239:1276	Strain J5903T shared typical chemotaxonomic characteristics with P. deserti 13S1-3T.
32833615	2	55	theme	surrounding	261:271	arg1	Nur					286:288	desertification land surrounding Jiuliancheng Nur	240:288	desertification land surrounding Jiuliancheng Nur in Hebei Province, PR China	240:316	A psychrotolerant actinobacterium, designated strain J5903T, was isolated from an alkaline soil sample from the rhizosphere of Suaeda salsa collected in desertification land surrounding Jiuliancheng Nur in Hebei Province, PR China.
32833615	8	56	theme	unidentified	797:808	arg1	glycolipid					810:819	one unidentified glycolipid	793:819	one unidentified glycolipid	793:819	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and one unidentified glycolipid.
32833615	2	57	theme	Jiuliancheng	273:284	arg1	Nur					286:288	desertification land surrounding Jiuliancheng Nur	240:288	desertification land surrounding Jiuliancheng Nur in Hebei Province, PR China	240:316	A psychrotolerant actinobacterium, designated strain J5903T, was isolated from an alkaline soil sample from the rhizosphere of Suaeda salsa collected in desertification land surrounding Jiuliancheng Nur in Hebei Province, PR China.
32833615	2	58	theme	land	256:259	arg1	Nur					286:288	desertification land surrounding Jiuliancheng Nur	240:288	desertification land surrounding Jiuliancheng Nur in Hebei Province, PR China	240:316	A psychrotolerant actinobacterium, designated strain J5903T, was isolated from an alkaline soil sample from the rhizosphere of Suaeda salsa collected in desertification land surrounding Jiuliancheng Nur in Hebei Province, PR China.
32833615	11	59	theme	highest	967:973	arg1	identity					994:1001	average nucleotide identity	975:1001	average nucleotide identity	975:1001	It shared the highest average nucleotide identity and digital DNA-DNA hybridization values with Planctomonas deserti 13S1-3T.
32833615	6	60	theme	muramyl	630:636	arg1	residue					638:644	The muramyl residue	626:644	The muramyl residue	626:644	The muramyl residue was acetyl type.
32833615	6	60	theme	muramyl	630:636	arg1	type					657:660	acetyl type	650:660	acetyl type	650:660	The muramyl residue was acetyl type.
32833615	8	61	theme	polar	726:730	arg1	diphosphatidylglycerol					744:765	diphosphatidylglycerol	744:765	diphosphatidylglycerol	744:765	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and one unidentified glycolipid.
32833615	8	61	theme	polar	726:730	arg1	lipids					732:737	The major polar lipids	716:737	The major polar lipids	716:737	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and one unidentified glycolipid.
32833615	14	62	theme	Planctomonas	1402:1413	arg1	species					1381:1387	a novel species	1373:1387	a novel species	1373:1387	Combining the polyphasic taxonomic evidence, strain J5903T represents a novel species of the genus Planctomonas, for which the name Planctomonas psychrotolerans sp.
32833615	11	63	dep	identity	994:1001	arg1	the					963:965	the	963:965	the	963:965	It shared the highest average nucleotide identity and digital DNA-DNA hybridization values with Planctomonas deserti 13S1-3T.
32833615	2	64	theme	desertification	240:254	arg1	Nur					286:288	desertification land surrounding Jiuliancheng Nur	240:288	desertification land surrounding Jiuliancheng Nur in Hebei Province, PR China	240:316	A psychrotolerant actinobacterium, designated strain J5903T, was isolated from an alkaline soil sample from the rhizosphere of Suaeda salsa collected in desertification land surrounding Jiuliancheng Nur in Hebei Province, PR China.
32833615	11	65	theme	digital	1007:1013	arg1	hybridization					1023:1035	digital DNA-DNA hybridization	1007:1035	digital DNA-DNA hybridization values	1007:1042	It shared the highest average nucleotide identity and digital DNA-DNA hybridization values with Planctomonas deserti 13S1-3T.
32833615	11	66	theme	Planctomonas	1049:1060	arg1	13S1-3T					1070:1076	Planctomonas deserti 13S1-3T	1049:1076	Planctomonas deserti 13S1-3T	1049:1076	It shared the highest average nucleotide identity and digital DNA-DNA hybridization values with Planctomonas deserti 13S1-3T.
32833615	14	67	theme	genus	1396:1400	arg1	Planctomonas					1402:1413	the genus Planctomonas	1392:1413	the genus Planctomonas	1392:1413	Combining the polyphasic taxonomic evidence, strain J5903T represents a novel species of the genus Planctomonas, for which the name Planctomonas psychrotolerans sp.
32833615	2	68	theme	strain	133:138	arg1	J5903T					140:145	strain J5903T	133:145	strain J5903T	133:145	A psychrotolerant actinobacterium, designated strain J5903T, was isolated from an alkaline soil sample from the rhizosphere of Suaeda salsa collected in desertification land surrounding Jiuliancheng Nur in Hebei Province, PR China.
32833615	12	69	theme	strain	1128:1133	arg1	J5903T					1135:1140	strain J5903T	1128:1140	strain J5903T	1128:1140	Phylogenies based on genome sequence showed that strain J5903T and P. deserti 13S1-3T formed a robust cluster with high bootstrap support.
32833615	13	70	theme	Strain	1218:1223	arg1	J5903T					1225:1230	Strain J5903T	1218:1230	Strain J5903T	1218:1230	Strain J5903T shared typical chemotaxonomic characteristics with P. deserti 13S1-3T.
32833615	11	71	theme	DNA-DNA	1015:1021	arg1	hybridization					1023:1035	digital DNA-DNA hybridization	1007:1035	digital DNA-DNA hybridization values	1007:1042	It shared the highest average nucleotide identity and digital DNA-DNA hybridization values with Planctomonas deserti 13S1-3T.
32833615	14	72	theme	novel	1375:1379	arg1	species					1381:1387	a novel species	1373:1387	a novel species	1373:1387	Combining the polyphasic taxonomic evidence, strain J5903T represents a novel species of the genus Planctomonas, for which the name Planctomonas psychrotolerans sp.
32359995	4	0	theme	Holstein	890:897	arg1	cows					899:902	early-lactation Holstein cows	874:902	early-lactation Holstein cows	874:902	This study aimed to estimate genetic parameters of blood BHB and NEFA predicted from milk MIR spectra and to assess their phenotypic and genetic correlations with milk production and composition traits in early-lactation Holstein cows.
32359995	7	1	theme	blood	1211:1215	arg1	metabolites					1217:1227	blood metabolites	1211:1227	blood metabolites	1211:1227	Genetic parameters of blood metabolites and milk traits were estimated for the whole observational period (5-35 DIM) and within 6 classes of DIM.
32359995	10	2	dep	similar	1717:1723	arg1	whereas					1814:1820	whereas	1814:1820	whereas	1814:1820	Genetic correlations of BHB with milk yield, SCS, protein percentage, lactose percentage, and urea nitrogen content were similar to, or at least in the same direction as, the correlations of NEFA with the same traits, whereas opposite correlations were observed with fat percentage and fat-to-protein ratio.
32359995	10	2	dep	similar	1717:1723	arg1	correlations					1771:1782	the correlations	1767:1782	the correlations of NEFA with the same traits	1767:1811	Genetic correlations of BHB with milk yield, SCS, protein percentage, lactose percentage, and urea nitrogen content were similar to, or at least in the same direction as, the correlations of NEFA with the same traits, whereas opposite correlations were observed with fat percentage and fat-to-protein ratio.
32359995	10	2	dep	similar	1717:1723	arg1	correlations					1604:1615	Genetic correlations	1596:1615	Genetic correlations of BHB with milk yield, SCS, protein percentage, lactose percentage, and urea nitrogen content	1596:1710	Genetic correlations of BHB with milk yield, SCS, protein percentage, lactose percentage, and urea nitrogen content were similar to, or at least in the same direction as, the correlations of NEFA with the same traits, whereas opposite correlations were observed with fat percentage and fat-to-protein ratio.
32359995	10	3	theme	same	1748:1751	arg1	direction					1753:1761	the same direction	1744:1761	the same direction	1744:1761	Genetic correlations of BHB with milk yield, SCS, protein percentage, lactose percentage, and urea nitrogen content were similar to, or at least in the same direction as, the correlations of NEFA with the same traits, whereas opposite correlations were observed with fat percentage and fat-to-protein ratio.
32359995	12	4	from	disorders	2226:2234	arg1	lactation					2245:2253	early lactation	2239:2253	early lactation	2239:2253	Therefore, they could be used as indicator traits of hyperketonemia in a selection index aimed to reduce the susceptibility of dairy cows to metabolic disorders in early lactation.
32359995	0	5	with	relationships	94:106	arg1	traits					118:123	milk traits	113:123	milk traits in early-lactation Holstein cows	113:156	Heritability estimates of predicted blood β-hydroxybutyrate and nonesterified fatty acids and relationships with milk traits in early-lactation Holstein cows.
32359995	10	6	theme	Genetic	1596:1602	arg1	correlations					1604:1615	Genetic correlations	1596:1615	Genetic correlations of BHB with milk yield, SCS, protein percentage, lactose percentage, and urea nitrogen content	1596:1710	Genetic correlations of BHB with milk yield, SCS, protein percentage, lactose percentage, and urea nitrogen content were similar to, or at least in the same direction as, the correlations of NEFA with the same traits, whereas opposite correlations were observed with fat percentage and fat-to-protein ratio.
32359995	10	6	theme	Genetic	1596:1602	arg1	correlations					1771:1782	the correlations	1767:1782	the correlations of NEFA with the same traits	1767:1811	Genetic correlations of BHB with milk yield, SCS, protein percentage, lactose percentage, and urea nitrogen content were similar to, or at least in the same direction as, the correlations of NEFA with the same traits, whereas opposite correlations were observed with fat percentage and fat-to-protein ratio.
32359995	12	7	theme	cows	2208:2211	arg1	susceptibility					2184:2197	the susceptibility	2180:2197	the susceptibility of dairy cows to metabolic disorders in early lactation	2180:2253	Therefore, they could be used as indicator traits of hyperketonemia in a selection index aimed to reduce the susceptibility of dairy cows to metabolic disorders in early lactation.
32359995	4	8	theme	composition	852:862	arg1	traits					864:869	composition traits	852:869	composition traits	852:869	This study aimed to estimate genetic parameters of blood BHB and NEFA predicted from milk MIR spectra and to assess their phenotypic and genetic correlations with milk production and composition traits in early-lactation Holstein cows.
32359995	6	9	theme	MIR	1131:1133	arg1	spectra					1135:1141	milk MIR spectra	1126:1141	milk MIR spectra using previously developed prediction models	1126:1186	Blood BHB and NEFA were predicted from milk MIR spectra using previously developed prediction models.
32359995	11	10	theme	genetic	2002:2008	arg1	variation					2010:2018	genetic variation	2002:2018	genetic variation that is potentially exploitable for breeding purposes	2002:2072	Results of the current study suggest that blood BHB and NEFA predicted from milk MIR spectra have genetic variation that is potentially exploitable for breeding purposes.
32359995	10	11	theme	same	1801:1804	arg1	traits					1806:1811	the same traits	1797:1811	the same traits	1797:1811	Genetic correlations of BHB with milk yield, SCS, protein percentage, lactose percentage, and urea nitrogen content were similar to, or at least in the same direction as, the correlations of NEFA with the same traits, whereas opposite correlations were observed with fat percentage and fat-to-protein ratio.
32359995	10	12	theme	protein	1646:1652	arg1	percentage					1654:1663	protein percentage	1646:1663	protein percentage	1646:1663	Genetic correlations of BHB with milk yield, SCS, protein percentage, lactose percentage, and urea nitrogen content were similar to, or at least in the same direction as, the correlations of NEFA with the same traits, whereas opposite correlations were observed with fat percentage and fat-to-protein ratio.
32359995	0	13	theme	early-lactation	128:142	arg1	cows					153:156	early-lactation Holstein cows	128:156	early-lactation Holstein cows	128:156	Heritability estimates of predicted blood β-hydroxybutyrate and nonesterified fatty acids and relationships with milk traits in early-lactation Holstein cows.
32359995	10	14	theme	opposite	1822:1829	arg1	correlations					1831:1842	opposite correlations	1822:1842	opposite correlations	1822:1842	Genetic correlations of BHB with milk yield, SCS, protein percentage, lactose percentage, and urea nitrogen content were similar to, or at least in the same direction as, the correlations of NEFA with the same traits, whereas opposite correlations were observed with fat percentage and fat-to-protein ratio.
32359995	11	15	theme	MIR	1985:1987	arg1	spectra					1989:1995	milk MIR spectra	1980:1995	milk MIR spectra	1980:1995	Results of the current study suggest that blood BHB and NEFA predicted from milk MIR spectra have genetic variation that is potentially exploitable for breeding purposes.
32359995	4	16	from	production	837:846	arg1	cows					899:902	early-lactation Holstein cows	874:902	early-lactation Holstein cows	874:902	This study aimed to estimate genetic parameters of blood BHB and NEFA predicted from milk MIR spectra and to assess their phenotypic and genetic correlations with milk production and composition traits in early-lactation Holstein cows.
32359995	1	17	theme	unbalanced	233:242	arg1	responsible					272:282	responsible	272:282	responsible	272:282	At the beginning of lactation, high-producing cows commonly experience an unbalanced energy status that is often responsible for the onset of metabolic disorders and impaired health and performance.
32359995	1	17	theme	unbalanced	233:242	arg1	status					251:256	an unbalanced energy status	230:256	an unbalanced energy status that is often responsible for the onset of metabolic disorders and impaired health and performance	230:355	At the beginning of lactation, high-producing cows commonly experience an unbalanced energy status that is often responsible for the onset of metabolic disorders and impaired health and performance.
32359995	11	18	theme	study	1927:1931	arg1	Results					1904:1910	Results	1904:1910	Results of the current study	1904:1931	Results of the current study suggest that blood BHB and NEFA predicted from milk MIR spectra have genetic variation that is potentially exploitable for breeding purposes.
32359995	4	19	theme	BHB	726:728	arg1	parameters					706:715	genetic parameters	698:715	genetic parameters of blood BHB and NEFA predicted from milk MIR spectra	698:769	This study aimed to estimate genetic parameters of blood BHB and NEFA predicted from milk MIR spectra and to assess their phenotypic and genetic correlations with milk production and composition traits in early-lactation Holstein cows.
32359995	5	20	theme	cows	1042:1045	arg1	n					1011:1011	n = 22,718	1011:1020	n = 22,718	1011:1020	The data set comprised the first test-day record within lactation and spectra of individual milk samples (n = 22,718) of 13,106 Holstein cows collected from 5 to 35 d in milk (DIM).
32359995	5	20	theme	cows	1042:1045	arg1	samples					1002:1008	individual milk samples	986:1008	individual milk samples (n = 22,718) of 13,106 Holstein cows collected from 5 to 35 d in milk (DIM)	986:1084	The data set comprised the first test-day record within lactation and spectra of individual milk samples (n = 22,718) of 13,106 Holstein cows collected from 5 to 35 d in milk (DIM).
32359995	3	21	theme	blood	600:604	arg1	BHB					606:608	blood BHB	600:608	blood BHB	600:608	Recently, prediction models based on mid-infrared (MIR) spectroscopy have been developed to assess blood BHB and NEFA from routinely collected individual milk samples.
32359995	5	22	theme	first	932:936	arg1	record					947:952	the first test-day record	928:952	the first test-day record within lactation and spectra of individual milk samples (n = 22,718) of 13,106 Holstein cows collected from 5 to 35 d in milk (DIM)	928:1084	The data set comprised the first test-day record within lactation and spectra of individual milk samples (n = 22,718) of 13,106 Holstein cows collected from 5 to 35 d in milk (DIM).
32359995	8	23	theme	highest	1408:1414	arg1	heritability					1416:1427	the highest heritability	1404:1427	the highest heritability in the first 10 d after calving (0.31 ± 0.06 and 0.19 ± 0.05 for BHB and NEFA, respectively)	1404:1520	Blood BHB and NEFA showed similar genetic variation across DIM, with the highest heritability in the first 10 d after calving (0.31 ± 0.06 and 0.19 ± 0.05 for BHB and NEFA, respectively).
32359995	4	24	theme	NEFA	734:737	arg1	parameters					706:715	genetic parameters	698:715	genetic parameters of blood BHB and NEFA predicted from milk MIR spectra	698:769	This study aimed to estimate genetic parameters of blood BHB and NEFA predicted from milk MIR spectra and to assess their phenotypic and genetic correlations with milk production and composition traits in early-lactation Holstein cows.
32359995	8	25	theme	Blood	1335:1339	arg1	BHB					1341:1343	Blood BHB	1335:1343	Blood BHB	1335:1343	Blood BHB and NEFA showed similar genetic variation across DIM, with the highest heritability in the first 10 d after calving (0.31 ± 0.06 and 0.19 ± 0.05 for BHB and NEFA, respectively).
32359995	3	26	theme	collected	634:642	arg1	samples					660:666	routinely collected individual milk samples	624:666	routinely collected individual milk samples	624:666	Recently, prediction models based on mid-infrared (MIR) spectroscopy have been developed to assess blood BHB and NEFA from routinely collected individual milk samples.
32359995	11	27	theme	breeding	2056:2063	arg1	purposes					2065:2072	breeding purposes	2056:2072	breeding purposes	2056:2072	Results of the current study suggest that blood BHB and NEFA predicted from milk MIR spectra have genetic variation that is potentially exploitable for breeding purposes.
32359995	12	28	theme	selection	2148:2156	arg1	index					2158:2162	a selection index	2146:2162	a selection index aimed to reduce the susceptibility of dairy cows to metabolic disorders in early lactation	2146:2253	Therefore, they could be used as indicator traits of hyperketonemia in a selection index aimed to reduce the susceptibility of dairy cows to metabolic disorders in early lactation.
32359995	6	29	theme	developed	1160:1168	arg1	models					1181:1186	previously developed prediction models	1149:1186	previously developed prediction models	1149:1186	Blood BHB and NEFA were predicted from milk MIR spectra using previously developed prediction models.
32359995	4	30	theme	genetic	698:704	arg1	parameters					706:715	genetic parameters	698:715	genetic parameters of blood BHB and NEFA predicted from milk MIR spectra	698:769	This study aimed to estimate genetic parameters of blood BHB and NEFA predicted from milk MIR spectra and to assess their phenotypic and genetic correlations with milk production and composition traits in early-lactation Holstein cows.
32359995	0	31	theme	fatty	78:82	arg1	acids					84:88	nonesterified fatty acids	64:88	nonesterified fatty acids	64:88	Heritability estimates of predicted blood β-hydroxybutyrate and nonesterified fatty acids and relationships with milk traits in early-lactation Holstein cows.
32359995	5	32	theme	data	909:912	arg1	set					914:916	The data set	905:916	The data set	905:916	The data set comprised the first test-day record within lactation and spectra of individual milk samples (n = 22,718) of 13,106 Holstein cows collected from 5 to 35 d in milk (DIM).
32359995	8	33	theme	first	1436:1440	arg1	d					1445:1445	the first 10 d	1432:1445	the first 10 d after calving (0.31 ± 0.06 and 0.19 ± 0.05 for BHB and NEFA, respectively)	1432:1520	Blood BHB and NEFA showed similar genetic variation across DIM, with the highest heritability in the first 10 d after calving (0.31 ± 0.06 and 0.19 ± 0.05 for BHB and NEFA, respectively).
32359995	10	34	theme	fat-to-protein	1882:1895	arg1	ratio					1897:1901	fat-to-protein ratio	1882:1901	fat-to-protein ratio	1882:1901	Genetic correlations of BHB with milk yield, SCS, protein percentage, lactose percentage, and urea nitrogen content were similar to, or at least in the same direction as, the correlations of NEFA with the same traits, whereas opposite correlations were observed with fat percentage and fat-to-protein ratio.
32359995	7	35	theme	Genetic	1189:1195	arg1	parameters					1197:1206	Genetic parameters	1189:1206	Genetic parameters of blood metabolites and milk traits	1189:1243	Genetic parameters of blood metabolites and milk traits were estimated for the whole observational period (5-35 DIM) and within 6 classes of DIM.
32359995	10	36	theme	nitrogen	1695:1702	arg1	content					1704:1710	urea nitrogen content	1690:1710	urea nitrogen content	1690:1710	Genetic correlations of BHB with milk yield, SCS, protein percentage, lactose percentage, and urea nitrogen content were similar to, or at least in the same direction as, the correlations of NEFA with the same traits, whereas opposite correlations were observed with fat percentage and fat-to-protein ratio.
32359995	5	37	theme	individual	986:995	arg1	n					1011:1011	n = 22,718	1011:1020	n = 22,718	1011:1020	The data set comprised the first test-day record within lactation and spectra of individual milk samples (n = 22,718) of 13,106 Holstein cows collected from 5 to 35 d in milk (DIM).
32359995	5	37	theme	individual	986:995	arg1	samples					1002:1008	individual milk samples	986:1008	individual milk samples (n = 22,718) of 13,106 Holstein cows collected from 5 to 35 d in milk (DIM)	986:1084	The data set comprised the first test-day record within lactation and spectra of individual milk samples (n = 22,718) of 13,106 Holstein cows collected from 5 to 35 d in milk (DIM).
32359995	5	38	theme	samples	1002:1008	arg1	spectra					975:981	spectra	975:981	spectra of individual milk samples (n = 22,718) of 13,106 Holstein cows collected from 5 to 35 d in milk (DIM)	975:1084	The data set comprised the first test-day record within lactation and spectra of individual milk samples (n = 22,718) of 13,106 Holstein cows collected from 5 to 35 d in milk (DIM).
32359995	5	38	theme	samples	1002:1008	arg1	lactation					961:969	lactation	961:969	lactation	961:969	The data set comprised the first test-day record within lactation and spectra of individual milk samples (n = 22,718) of 13,106 Holstein cows collected from 5 to 35 d in milk (DIM).
32359995	2	39	theme	mobilization	457:468	arg1	acids					412:416	nonesterified fatty acids	392:416	nonesterified fatty acids (NEFA)	392:423	Blood β-hydroxybutyrate (BHB) and nonesterified fatty acids (NEFA) are indicators of excessive fat mobilization and circulating ketone bodies.
32359995	2	39	theme	mobilization	457:468	arg1	β-hydroxybutyrate					364:380	Blood β-hydroxybutyrate	358:380	Blood β-hydroxybutyrate (BHB)	358:386	Blood β-hydroxybutyrate (BHB) and nonesterified fatty acids (NEFA) are indicators of excessive fat mobilization and circulating ketone bodies.
32359995	2	39	theme	mobilization	457:468	arg1	indicators					429:438	indicators	429:438	indicators of excessive fat mobilization and circulating ketone bodies	429:498	Blood β-hydroxybutyrate (BHB) and nonesterified fatty acids (NEFA) are indicators of excessive fat mobilization and circulating ketone bodies.
32359995	2	40	theme	fatty	406:410	arg1	acids					412:416	nonesterified fatty acids	392:416	nonesterified fatty acids (NEFA)	392:423	Blood β-hydroxybutyrate (BHB) and nonesterified fatty acids (NEFA) are indicators of excessive fat mobilization and circulating ketone bodies.
32359995	2	40	theme	fatty	406:410	arg1	β-hydroxybutyrate					364:380	Blood β-hydroxybutyrate	358:380	Blood β-hydroxybutyrate (BHB)	358:386	Blood β-hydroxybutyrate (BHB) and nonesterified fatty acids (NEFA) are indicators of excessive fat mobilization and circulating ketone bodies.
32359995	2	40	theme	fatty	406:410	arg1	NEFA					419:422	NEFA	419:422	NEFA	419:422	Blood β-hydroxybutyrate (BHB) and nonesterified fatty acids (NEFA) are indicators of excessive fat mobilization and circulating ketone bodies.
32359995	2	40	theme	fatty	406:410	arg1	indicators					429:438	indicators	429:438	indicators of excessive fat mobilization and circulating ketone bodies	429:498	Blood β-hydroxybutyrate (BHB) and nonesterified fatty acids (NEFA) are indicators of excessive fat mobilization and circulating ketone bodies.
32359995	0	41	theme	Heritability	0:11	arg1	estimates					13:21	Heritability estimates	0:21	Heritability estimates of predicted blood β-hydroxybutyrate and nonesterified fatty acids	0:88	Heritability estimates of predicted blood β-hydroxybutyrate and nonesterified fatty acids and relationships with milk traits in early-lactation Holstein cows.
32359995	2	42	theme	excessive	443:451	arg1	mobilization					457:468	excessive fat mobilization	443:468	excessive fat mobilization	443:468	Blood β-hydroxybutyrate (BHB) and nonesterified fatty acids (NEFA) are indicators of excessive fat mobilization and circulating ketone bodies.
32359995	4	43	theme	MIR	759:761	arg1	spectra					763:769	milk MIR spectra	754:769	milk MIR spectra	754:769	This study aimed to estimate genetic parameters of blood BHB and NEFA predicted from milk MIR spectra and to assess their phenotypic and genetic correlations with milk production and composition traits in early-lactation Holstein cows.
32359995	1	44	theme	metabolic	301:309	arg1	disorders					311:319	metabolic disorders	301:319	metabolic disorders	301:319	At the beginning of lactation, high-producing cows commonly experience an unbalanced energy status that is often responsible for the onset of metabolic disorders and impaired health and performance.
32359995	2	45	theme	bodies	493:498	arg1	acids					412:416	nonesterified fatty acids	392:416	nonesterified fatty acids (NEFA)	392:423	Blood β-hydroxybutyrate (BHB) and nonesterified fatty acids (NEFA) are indicators of excessive fat mobilization and circulating ketone bodies.
32359995	2	45	theme	bodies	493:498	arg1	β-hydroxybutyrate					364:380	Blood β-hydroxybutyrate	358:380	Blood β-hydroxybutyrate (BHB)	358:386	Blood β-hydroxybutyrate (BHB) and nonesterified fatty acids (NEFA) are indicators of excessive fat mobilization and circulating ketone bodies.
32359995	2	45	theme	bodies	493:498	arg1	indicators					429:438	indicators	429:438	indicators of excessive fat mobilization and circulating ketone bodies	429:498	Blood β-hydroxybutyrate (BHB) and nonesterified fatty acids (NEFA) are indicators of excessive fat mobilization and circulating ketone bodies.
32359995	0	46	theme	blood	36:40	arg1	β-hydroxybutyrate					42:58	predicted blood β-hydroxybutyrate	26:58	predicted blood β-hydroxybutyrate	26:58	Heritability estimates of predicted blood β-hydroxybutyrate and nonesterified fatty acids and relationships with milk traits in early-lactation Holstein cows.
32359995	7	47	theme	whole	1268:1272	arg1	period					1288:1293	the whole observational period	1264:1293	the whole observational period (5-35 DIM)	1264:1304	Genetic parameters of blood metabolites and milk traits were estimated for the whole observational period (5-35 DIM) and within 6 classes of DIM.
32359995	7	47	theme	whole	1268:1272	arg1	DIM					1301:1303	5-35 DIM	1296:1303	5-35 DIM	1296:1303	Genetic parameters of blood metabolites and milk traits were estimated for the whole observational period (5-35 DIM) and within 6 classes of DIM.
32359995	2	48	theme	circulating	474:484	arg1	bodies					493:498	circulating ketone bodies	474:498	circulating ketone bodies	474:498	Blood β-hydroxybutyrate (BHB) and nonesterified fatty acids (NEFA) are indicators of excessive fat mobilization and circulating ketone bodies.
32359995	12	49	theme	early	2239:2243	arg1	lactation					2245:2253	early lactation	2239:2253	early lactation	2239:2253	Therefore, they could be used as indicator traits of hyperketonemia in a selection index aimed to reduce the susceptibility of dairy cows to metabolic disorders in early lactation.
32359995	3	50	theme	milk	655:658	arg1	samples					660:666	routinely collected individual milk samples	624:666	routinely collected individual milk samples	624:666	Recently, prediction models based on mid-infrared (MIR) spectroscopy have been developed to assess blood BHB and NEFA from routinely collected individual milk samples.
32359995	9	51	dep	moderate	1572:1579	arg1	moderate					1572:1579	moderate	1572:1579	moderate	1572:1579	The genetic correlation between BHB and NEFA was moderate (0.51 ± 0.05).
32359995	9	51	dep	moderate	1572:1579	arg1	±					1587:1587	0.51 ± 0.05	1582:1592	0.51 ± 0.05	1582:1592	The genetic correlation between BHB and NEFA was moderate (0.51 ± 0.05).
32359995	9	51	dep	moderate	1572:1579	arg1	correlation					1535:1545	The genetic correlation	1523:1545	The genetic correlation between BHB and NEFA	1523:1566	The genetic correlation between BHB and NEFA was moderate (0.51 ± 0.05).
32359995	1	52	theme	health	334:339	arg1	onset					292:296	the onset	288:296	the onset of metabolic disorders and impaired health and performance	288:355	At the beginning of lactation, high-producing cows commonly experience an unbalanced energy status that is often responsible for the onset of metabolic disorders and impaired health and performance.
32359995	10	53	with	correlations	1604:1615	arg1	yield					1634:1638	milk yield	1629:1638	milk yield	1629:1638	Genetic correlations of BHB with milk yield, SCS, protein percentage, lactose percentage, and urea nitrogen content were similar to, or at least in the same direction as, the correlations of NEFA with the same traits, whereas opposite correlations were observed with fat percentage and fat-to-protein ratio.
32359995	10	53	with	correlations	1604:1615	arg1	percentage					1674:1683	lactose percentage	1666:1683	lactose percentage	1666:1683	Genetic correlations of BHB with milk yield, SCS, protein percentage, lactose percentage, and urea nitrogen content were similar to, or at least in the same direction as, the correlations of NEFA with the same traits, whereas opposite correlations were observed with fat percentage and fat-to-protein ratio.
32359995	10	53	with	correlations	1604:1615	arg1	percentage					1654:1663	protein percentage	1646:1663	protein percentage	1646:1663	Genetic correlations of BHB with milk yield, SCS, protein percentage, lactose percentage, and urea nitrogen content were similar to, or at least in the same direction as, the correlations of NEFA with the same traits, whereas opposite correlations were observed with fat percentage and fat-to-protein ratio.
32359995	10	53	with	correlations	1604:1615	arg1	SCS					1641:1643	SCS	1641:1643	SCS	1641:1643	Genetic correlations of BHB with milk yield, SCS, protein percentage, lactose percentage, and urea nitrogen content were similar to, or at least in the same direction as, the correlations of NEFA with the same traits, whereas opposite correlations were observed with fat percentage and fat-to-protein ratio.
32359995	10	53	with	correlations	1604:1615	arg1	content					1704:1710	urea nitrogen content	1690:1710	urea nitrogen content	1690:1710	Genetic correlations of BHB with milk yield, SCS, protein percentage, lactose percentage, and urea nitrogen content were similar to, or at least in the same direction as, the correlations of NEFA with the same traits, whereas opposite correlations were observed with fat percentage and fat-to-protein ratio.
32359995	5	54	theme	Holstein	1033:1040	arg1	cows					1042:1045	13,106 Holstein cows	1026:1045	13,106 Holstein cows	1026:1045	The data set comprised the first test-day record within lactation and spectra of individual milk samples (n = 22,718) of 13,106 Holstein cows collected from 5 to 35 d in milk (DIM).
32359995	8	55	theme	genetic	1369:1375	arg1	variation					1377:1385	similar genetic variation	1361:1385	similar genetic variation across DIM	1361:1396	Blood BHB and NEFA showed similar genetic variation across DIM, with the highest heritability in the first 10 d after calving (0.31 ± 0.06 and 0.19 ± 0.05 for BHB and NEFA, respectively).
32359995	1	56	theme	energy	244:249	arg1	responsible					272:282	responsible	272:282	responsible	272:282	At the beginning of lactation, high-producing cows commonly experience an unbalanced energy status that is often responsible for the onset of metabolic disorders and impaired health and performance.
32359995	1	56	theme	energy	244:249	arg1	status					251:256	an unbalanced energy status	230:256	an unbalanced energy status that is often responsible for the onset of metabolic disorders and impaired health and performance	230:355	At the beginning of lactation, high-producing cows commonly experience an unbalanced energy status that is often responsible for the onset of metabolic disorders and impaired health and performance.
32359995	0	57	from	traits	118:123	arg1	cows					153:156	early-lactation Holstein cows	128:156	early-lactation Holstein cows	128:156	Heritability estimates of predicted blood β-hydroxybutyrate and nonesterified fatty acids and relationships with milk traits in early-lactation Holstein cows.
32359995	10	58	with	correlations	1771:1782	arg1	traits					1806:1811	the same traits	1797:1811	the same traits	1797:1811	Genetic correlations of BHB with milk yield, SCS, protein percentage, lactose percentage, and urea nitrogen content were similar to, or at least in the same direction as, the correlations of NEFA with the same traits, whereas opposite correlations were observed with fat percentage and fat-to-protein ratio.
32359995	4	59	theme	genetic	806:812	arg1	correlations					814:825	their phenotypic and genetic correlations	785:825	their phenotypic and genetic correlations with milk production and composition traits in early-lactation Holstein cows	785:902	This study aimed to estimate genetic parameters of blood BHB and NEFA predicted from milk MIR spectra and to assess their phenotypic and genetic correlations with milk production and composition traits in early-lactation Holstein cows.
32359995	10	60	theme	BHB	1620:1622	arg1	correlations					1604:1615	Genetic correlations	1596:1615	Genetic correlations of BHB with milk yield, SCS, protein percentage, lactose percentage, and urea nitrogen content	1596:1710	Genetic correlations of BHB with milk yield, SCS, protein percentage, lactose percentage, and urea nitrogen content were similar to, or at least in the same direction as, the correlations of NEFA with the same traits, whereas opposite correlations were observed with fat percentage and fat-to-protein ratio.
32359995	10	60	theme	BHB	1620:1622	arg1	correlations					1771:1782	the correlations	1767:1782	the correlations of NEFA with the same traits	1767:1811	Genetic correlations of BHB with milk yield, SCS, protein percentage, lactose percentage, and urea nitrogen content were similar to, or at least in the same direction as, the correlations of NEFA with the same traits, whereas opposite correlations were observed with fat percentage and fat-to-protein ratio.
32359995	7	61	theme	milk	1233:1236	arg1	traits					1238:1243	milk traits	1233:1243	milk traits	1233:1243	Genetic parameters of blood metabolites and milk traits were estimated for the whole observational period (5-35 DIM) and within 6 classes of DIM.
32359995	5	62	dep	35	1067:1068	arg1	to					1064:1065	to	1064:1065	to	1064:1065	The data set comprised the first test-day record within lactation and spectra of individual milk samples (n = 22,718) of 13,106 Holstein cows collected from 5 to 35 d in milk (DIM).
32359995	12	63	theme	indicator	2108:2116	arg1	traits					2118:2123	indicator traits	2108:2123	indicator traits of hyperketonemia	2108:2141	Therefore, they could be used as indicator traits of hyperketonemia in a selection index aimed to reduce the susceptibility of dairy cows to metabolic disorders in early lactation.
32359995	12	63	theme	indicator	2108:2116	arg1	they					2086:2089	they	2086:2089	they	2086:2089	Therefore, they could be used as indicator traits of hyperketonemia in a selection index aimed to reduce the susceptibility of dairy cows to metabolic disorders in early lactation.
32359995	4	64	theme	early-lactation	874:888	arg1	cows					899:902	early-lactation Holstein cows	874:902	early-lactation Holstein cows	874:902	This study aimed to estimate genetic parameters of blood BHB and NEFA predicted from milk MIR spectra and to assess their phenotypic and genetic correlations with milk production and composition traits in early-lactation Holstein cows.
32359995	7	65	theme	metabolites	1217:1227	arg1	parameters					1197:1206	Genetic parameters	1189:1206	Genetic parameters of blood metabolites and milk traits	1189:1243	Genetic parameters of blood metabolites and milk traits were estimated for the whole observational period (5-35 DIM) and within 6 classes of DIM.
32359995	11	66	theme	blood	1946:1950	arg1	BHB					1952:1954	blood BHB	1946:1954	blood BHB	1946:1954	Results of the current study suggest that blood BHB and NEFA predicted from milk MIR spectra have genetic variation that is potentially exploitable for breeding purposes.
32359995	12	67	theme	dairy	2202:2206	arg1	cows					2208:2211	dairy cows	2202:2211	dairy cows	2202:2211	Therefore, they could be used as indicator traits of hyperketonemia in a selection index aimed to reduce the susceptibility of dairy cows to metabolic disorders in early lactation.
32359995	3	68	from	samples	660:666	arg1	BHB					606:608	blood BHB	600:608	blood BHB	600:608	Recently, prediction models based on mid-infrared (MIR) spectroscopy have been developed to assess blood BHB and NEFA from routinely collected individual milk samples.
32359995	3	68	from	samples	660:666	arg1	NEFA					614:617	NEFA	614:617	NEFA	614:617	Recently, prediction models based on mid-infrared (MIR) spectroscopy have been developed to assess blood BHB and NEFA from routinely collected individual milk samples.
32359995	10	69	theme	milk	1629:1632	arg1	yield					1634:1638	milk yield	1629:1638	milk yield	1629:1638	Genetic correlations of BHB with milk yield, SCS, protein percentage, lactose percentage, and urea nitrogen content were similar to, or at least in the same direction as, the correlations of NEFA with the same traits, whereas opposite correlations were observed with fat percentage and fat-to-protein ratio.
32359995	1	70	theme	lactation	179:187	arg1	beginning					166:174	the beginning	162:174	the beginning of lactation	162:187	At the beginning of lactation, high-producing cows commonly experience an unbalanced energy status that is often responsible for the onset of metabolic disorders and impaired health and performance.
32359995	11	71	theme	milk	1980:1983	arg1	spectra					1989:1995	milk MIR spectra	1980:1995	milk MIR spectra	1980:1995	Results of the current study suggest that blood BHB and NEFA predicted from milk MIR spectra have genetic variation that is potentially exploitable for breeding purposes.
32359995	6	72	theme	milk	1126:1129	arg1	spectra					1135:1141	milk MIR spectra	1126:1141	milk MIR spectra using previously developed prediction models	1126:1186	Blood BHB and NEFA were predicted from milk MIR spectra using previously developed prediction models.
32359995	1	73	theme	high-producing	190:203	arg1	cows					205:208	high-producing cows	190:208	high-producing cows	190:208	At the beginning of lactation, high-producing cows commonly experience an unbalanced energy status that is often responsible for the onset of metabolic disorders and impaired health and performance.
32359995	0	74	theme	Holstein	144:151	arg1	cows					153:156	early-lactation Holstein cows	128:156	early-lactation Holstein cows	128:156	Heritability estimates of predicted blood β-hydroxybutyrate and nonesterified fatty acids and relationships with milk traits in early-lactation Holstein cows.
32359995	2	75	theme	Blood	358:362	arg1	acids					412:416	nonesterified fatty acids	392:416	nonesterified fatty acids (NEFA)	392:423	Blood β-hydroxybutyrate (BHB) and nonesterified fatty acids (NEFA) are indicators of excessive fat mobilization and circulating ketone bodies.
32359995	2	75	theme	Blood	358:362	arg1	BHB					383:385	BHB	383:385	BHB	383:385	Blood β-hydroxybutyrate (BHB) and nonesterified fatty acids (NEFA) are indicators of excessive fat mobilization and circulating ketone bodies.
32359995	2	75	theme	Blood	358:362	arg1	β-hydroxybutyrate					364:380	Blood β-hydroxybutyrate	358:380	Blood β-hydroxybutyrate (BHB)	358:386	Blood β-hydroxybutyrate (BHB) and nonesterified fatty acids (NEFA) are indicators of excessive fat mobilization and circulating ketone bodies.
32359995	2	75	theme	Blood	358:362	arg1	indicators					429:438	indicators	429:438	indicators of excessive fat mobilization and circulating ketone bodies	429:498	Blood β-hydroxybutyrate (BHB) and nonesterified fatty acids (NEFA) are indicators of excessive fat mobilization and circulating ketone bodies.
32359995	5	76	from	d	1070:1070	arg1	DIM					1081:1083	DIM	1081:1083	DIM	1081:1083	The data set comprised the first test-day record within lactation and spectra of individual milk samples (n = 22,718) of 13,106 Holstein cows collected from 5 to 35 d in milk (DIM).
32359995	5	76	from	d	1070:1070	arg1	milk					1075:1078	milk	1075:1078	milk (DIM)	1075:1084	The data set comprised the first test-day record within lactation and spectra of individual milk samples (n = 22,718) of 13,106 Holstein cows collected from 5 to 35 d in milk (DIM).
32359995	10	77	theme	lactose	1666:1672	arg1	percentage					1674:1683	lactose percentage	1666:1683	lactose percentage	1666:1683	Genetic correlations of BHB with milk yield, SCS, protein percentage, lactose percentage, and urea nitrogen content were similar to, or at least in the same direction as, the correlations of NEFA with the same traits, whereas opposite correlations were observed with fat percentage and fat-to-protein ratio.
32359995	7	78	theme	DIM	1330:1332	arg1	classes					1319:1325	6 classes	1317:1325	6 classes of DIM	1317:1332	Genetic parameters of blood metabolites and milk traits were estimated for the whole observational period (5-35 DIM) and within 6 classes of DIM.
32359995	4	79	theme	blood	720:724	arg1	BHB					726:728	blood BHB and NEFA	720:737	BHB	726:728	This study aimed to estimate genetic parameters of blood BHB and NEFA predicted from milk MIR spectra and to assess their phenotypic and genetic correlations with milk production and composition traits in early-lactation Holstein cows.
32359995	4	80	theme	phenotypic	791:800	arg1	correlations					814:825	their phenotypic and genetic correlations	785:825	their phenotypic and genetic correlations with milk production and composition traits in early-lactation Holstein cows	785:902	This study aimed to estimate genetic parameters of blood BHB and NEFA predicted from milk MIR spectra and to assess their phenotypic and genetic correlations with milk production and composition traits in early-lactation Holstein cows.
32359995	5	81	theme	test-day	938:945	arg1	record					947:952	the first test-day record	928:952	the first test-day record within lactation and spectra of individual milk samples (n = 22,718) of 13,106 Holstein cows collected from 5 to 35 d in milk (DIM)	928:1084	The data set comprised the first test-day record within lactation and spectra of individual milk samples (n = 22,718) of 13,106 Holstein cows collected from 5 to 35 d in milk (DIM).
32359995	11	82	theme	current	1919:1925	arg1	study					1927:1931	the current study	1915:1931	the current study	1915:1931	Results of the current study suggest that blood BHB and NEFA predicted from milk MIR spectra have genetic variation that is potentially exploitable for breeding purposes.
32359995	6	83	theme	prediction	1170:1179	arg1	models					1181:1186	previously developed prediction models	1149:1186	previously developed prediction models	1149:1186	Blood BHB and NEFA were predicted from milk MIR spectra using previously developed prediction models.
32359995	6	84	theme	Blood	1087:1091	arg1	BHB					1093:1095	Blood BHB	1087:1095	Blood BHB	1087:1095	Blood BHB and NEFA were predicted from milk MIR spectra using previously developed prediction models.
32359995	0	85	theme	acids	84:88	arg1	relationships					94:106	relationships	94:106	relationships with milk traits in early-lactation Holstein cows	94:156	Heritability estimates of predicted blood β-hydroxybutyrate and nonesterified fatty acids and relationships with milk traits in early-lactation Holstein cows.
32359995	0	85	theme	acids	84:88	arg1	estimates					13:21	Heritability estimates	0:21	Heritability estimates of predicted blood β-hydroxybutyrate and nonesterified fatty acids	0:88	Heritability estimates of predicted blood β-hydroxybutyrate and nonesterified fatty acids and relationships with milk traits in early-lactation Holstein cows.
32359995	10	86	theme	urea	1690:1693	arg1	content					1704:1710	urea nitrogen content	1690:1710	urea nitrogen content	1690:1710	Genetic correlations of BHB with milk yield, SCS, protein percentage, lactose percentage, and urea nitrogen content were similar to, or at least in the same direction as, the correlations of NEFA with the same traits, whereas opposite correlations were observed with fat percentage and fat-to-protein ratio.
32359995	4	87	with	correlations	814:825	arg1	traits					864:869	composition traits	852:869	composition traits	852:869	This study aimed to estimate genetic parameters of blood BHB and NEFA predicted from milk MIR spectra and to assess their phenotypic and genetic correlations with milk production and composition traits in early-lactation Holstein cows.
32359995	4	87	with	correlations	814:825	arg1	production					837:846	milk production	832:846	milk production	832:846	This study aimed to estimate genetic parameters of blood BHB and NEFA predicted from milk MIR spectra and to assess their phenotypic and genetic correlations with milk production and composition traits in early-lactation Holstein cows.
32359995	12	88	used	used	2100:2103	arg2	they					2086:2089	they	2086:2089	they	2086:2089	Therefore, they could be used as indicator traits of hyperketonemia in a selection index aimed to reduce the susceptibility of dairy cows to metabolic disorders in early lactation.
32359995	12	88	used	used	2100:2103	arg2	traits					2118:2123	indicator traits	2108:2123	indicator traits of hyperketonemia	2108:2141	Therefore, they could be used as indicator traits of hyperketonemia in a selection index aimed to reduce the susceptibility of dairy cows to metabolic disorders in early lactation.
32359995	12	89	theme	hyperketonemia	2128:2141	arg1	traits					2118:2123	indicator traits	2108:2123	indicator traits of hyperketonemia	2108:2141	Therefore, they could be used as indicator traits of hyperketonemia in a selection index aimed to reduce the susceptibility of dairy cows to metabolic disorders in early lactation.
32359995	12	89	theme	hyperketonemia	2128:2141	arg1	they					2086:2089	they	2086:2089	they	2086:2089	Therefore, they could be used as indicator traits of hyperketonemia in a selection index aimed to reduce the susceptibility of dairy cows to metabolic disorders in early lactation.
32359995	0	90	theme	milk	113:116	arg1	traits					118:123	milk traits	113:123	milk traits in early-lactation Holstein cows	113:156	Heritability estimates of predicted blood β-hydroxybutyrate and nonesterified fatty acids and relationships with milk traits in early-lactation Holstein cows.
32359995	10	91	theme	fat	1863:1865	arg1	percentage					1867:1876	fat percentage	1863:1876	fat percentage	1863:1876	Genetic correlations of BHB with milk yield, SCS, protein percentage, lactose percentage, and urea nitrogen content were similar to, or at least in the same direction as, the correlations of NEFA with the same traits, whereas opposite correlations were observed with fat percentage and fat-to-protein ratio.
32359995	8	92	from	heritability	1416:1427	arg1	d					1445:1445	the first 10 d	1432:1445	the first 10 d after calving (0.31 ± 0.06 and 0.19 ± 0.05 for BHB and NEFA, respectively)	1432:1520	Blood BHB and NEFA showed similar genetic variation across DIM, with the highest heritability in the first 10 d after calving (0.31 ± 0.06 and 0.19 ± 0.05 for BHB and NEFA, respectively).
32359995	4	93	from	traits	864:869	arg1	cows					899:902	early-lactation Holstein cows	874:902	early-lactation Holstein cows	874:902	This study aimed to estimate genetic parameters of blood BHB and NEFA predicted from milk MIR spectra and to assess their phenotypic and genetic correlations with milk production and composition traits in early-lactation Holstein cows.
32359995	5	94	theme	milk	997:1000	arg1	n					1011:1011	n = 22,718	1011:1020	n = 22,718	1011:1020	The data set comprised the first test-day record within lactation and spectra of individual milk samples (n = 22,718) of 13,106 Holstein cows collected from 5 to 35 d in milk (DIM).
32359995	5	94	theme	milk	997:1000	arg1	samples					1002:1008	individual milk samples	986:1008	individual milk samples (n = 22,718) of 13,106 Holstein cows collected from 5 to 35 d in milk (DIM)	986:1084	The data set comprised the first test-day record within lactation and spectra of individual milk samples (n = 22,718) of 13,106 Holstein cows collected from 5 to 35 d in milk (DIM).
32359995	10	95	theme	NEFA	1787:1790	arg1	correlations					1604:1615	Genetic correlations	1596:1615	Genetic correlations of BHB with milk yield, SCS, protein percentage, lactose percentage, and urea nitrogen content	1596:1710	Genetic correlations of BHB with milk yield, SCS, protein percentage, lactose percentage, and urea nitrogen content were similar to, or at least in the same direction as, the correlations of NEFA with the same traits, whereas opposite correlations were observed with fat percentage and fat-to-protein ratio.
32359995	10	95	theme	NEFA	1787:1790	arg1	correlations					1771:1782	the correlations	1767:1782	the correlations of NEFA with the same traits	1767:1811	Genetic correlations of BHB with milk yield, SCS, protein percentage, lactose percentage, and urea nitrogen content were similar to, or at least in the same direction as, the correlations of NEFA with the same traits, whereas opposite correlations were observed with fat percentage and fat-to-protein ratio.
32359995	2	96	theme	fat	453:455	arg1	mobilization					457:468	excessive fat mobilization	443:468	excessive fat mobilization	443:468	Blood β-hydroxybutyrate (BHB) and nonesterified fatty acids (NEFA) are indicators of excessive fat mobilization and circulating ketone bodies.
32359995	2	97	theme	nonesterified	392:404	arg1	acids					412:416	nonesterified fatty acids	392:416	nonesterified fatty acids (NEFA)	392:423	Blood β-hydroxybutyrate (BHB) and nonesterified fatty acids (NEFA) are indicators of excessive fat mobilization and circulating ketone bodies.
32359995	2	97	theme	nonesterified	392:404	arg1	β-hydroxybutyrate					364:380	Blood β-hydroxybutyrate	358:380	Blood β-hydroxybutyrate (BHB)	358:386	Blood β-hydroxybutyrate (BHB) and nonesterified fatty acids (NEFA) are indicators of excessive fat mobilization and circulating ketone bodies.
32359995	2	97	theme	nonesterified	392:404	arg1	NEFA					419:422	NEFA	419:422	NEFA	419:422	Blood β-hydroxybutyrate (BHB) and nonesterified fatty acids (NEFA) are indicators of excessive fat mobilization and circulating ketone bodies.
32359995	2	97	theme	nonesterified	392:404	arg1	indicators					429:438	indicators	429:438	indicators of excessive fat mobilization and circulating ketone bodies	429:498	Blood β-hydroxybutyrate (BHB) and nonesterified fatty acids (NEFA) are indicators of excessive fat mobilization and circulating ketone bodies.
32359995	0	98	theme	predicted	26:34	arg1	β-hydroxybutyrate					42:58	predicted blood β-hydroxybutyrate	26:58	predicted blood β-hydroxybutyrate	26:58	Heritability estimates of predicted blood β-hydroxybutyrate and nonesterified fatty acids and relationships with milk traits in early-lactation Holstein cows.
32359995	9	99	theme	genetic	1527:1533	arg1	moderate					1572:1579	moderate	1572:1579	moderate	1572:1579	The genetic correlation between BHB and NEFA was moderate (0.51 ± 0.05).
32359995	9	99	theme	genetic	1527:1533	arg1	±					1587:1587	0.51 ± 0.05	1582:1592	0.51 ± 0.05	1582:1592	The genetic correlation between BHB and NEFA was moderate (0.51 ± 0.05).
32359995	9	99	theme	genetic	1527:1533	arg1	correlation					1535:1545	The genetic correlation	1523:1545	The genetic correlation between BHB and NEFA	1523:1566	The genetic correlation between BHB and NEFA was moderate (0.51 ± 0.05).
32359995	7	100	theme	observational	1274:1286	arg1	period					1288:1293	the whole observational period	1264:1293	the whole observational period (5-35 DIM)	1264:1304	Genetic parameters of blood metabolites and milk traits were estimated for the whole observational period (5-35 DIM) and within 6 classes of DIM.
32359995	7	100	theme	observational	1274:1286	arg1	DIM					1301:1303	5-35 DIM	1296:1303	5-35 DIM	1296:1303	Genetic parameters of blood metabolites and milk traits were estimated for the whole observational period (5-35 DIM) and within 6 classes of DIM.
32359995	4	101	theme	milk	754:757	arg1	spectra					763:769	milk MIR spectra	754:769	milk MIR spectra	754:769	This study aimed to estimate genetic parameters of blood BHB and NEFA predicted from milk MIR spectra and to assess their phenotypic and genetic correlations with milk production and composition traits in early-lactation Holstein cows.
32359995	0	102	theme	β-hydroxybutyrate	42:58	arg1	relationships					94:106	relationships	94:106	relationships with milk traits in early-lactation Holstein cows	94:156	Heritability estimates of predicted blood β-hydroxybutyrate and nonesterified fatty acids and relationships with milk traits in early-lactation Holstein cows.
32359995	0	102	theme	β-hydroxybutyrate	42:58	arg1	estimates					13:21	Heritability estimates	0:21	Heritability estimates of predicted blood β-hydroxybutyrate and nonesterified fatty acids	0:88	Heritability estimates of predicted blood β-hydroxybutyrate and nonesterified fatty acids and relationships with milk traits in early-lactation Holstein cows.
32359995	1	103	theme	disorders	311:319	arg1	onset					292:296	the onset	288:296	the onset of metabolic disorders and impaired health and performance	288:355	At the beginning of lactation, high-producing cows commonly experience an unbalanced energy status that is often responsible for the onset of metabolic disorders and impaired health and performance.
32359995	2	104	theme	ketone	486:491	arg1	bodies					493:498	circulating ketone bodies	474:498	circulating ketone bodies	474:498	Blood β-hydroxybutyrate (BHB) and nonesterified fatty acids (NEFA) are indicators of excessive fat mobilization and circulating ketone bodies.
32359995	3	105	theme	individual	644:653	arg1	samples					660:666	routinely collected individual milk samples	624:666	routinely collected individual milk samples	624:666	Recently, prediction models based on mid-infrared (MIR) spectroscopy have been developed to assess blood BHB and NEFA from routinely collected individual milk samples.
32359995	0	106	theme	nonesterified	64:76	arg1	acids					84:88	nonesterified fatty acids	64:88	nonesterified fatty acids	64:88	Heritability estimates of predicted blood β-hydroxybutyrate and nonesterified fatty acids and relationships with milk traits in early-lactation Holstein cows.
32359995	1	107	theme	impaired	325:332	arg1	health					334:339	impaired health	325:339	impaired health	325:339	At the beginning of lactation, high-producing cows commonly experience an unbalanced energy status that is often responsible for the onset of metabolic disorders and impaired health and performance.
32359995	4	108	theme	milk	832:835	arg1	production					837:846	milk production	832:846	milk production	832:846	This study aimed to estimate genetic parameters of blood BHB and NEFA predicted from milk MIR spectra and to assess their phenotypic and genetic correlations with milk production and composition traits in early-lactation Holstein cows.
32359995	0	109	with	estimates	13:21	arg1	traits					118:123	milk traits	113:123	milk traits in early-lactation Holstein cows	113:156	Heritability estimates of predicted blood β-hydroxybutyrate and nonesterified fatty acids and relationships with milk traits in early-lactation Holstein cows.
32359995	3	110	theme	prediction	511:520	arg1	models					522:527	prediction models	511:527	prediction models based on mid-infrared (MIR) spectroscopy	511:568	Recently, prediction models based on mid-infrared (MIR) spectroscopy have been developed to assess blood BHB and NEFA from routinely collected individual milk samples.
32359995	7	111	theme	traits	1238:1243	arg1	parameters					1197:1206	Genetic parameters	1189:1206	Genetic parameters of blood metabolites and milk traits	1189:1243	Genetic parameters of blood metabolites and milk traits were estimated for the whole observational period (5-35 DIM) and within 6 classes of DIM.
32359995	12	112	theme	metabolic	2216:2224	arg1	disorders					2226:2234	metabolic disorders	2216:2234	metabolic disorders in early lactation	2216:2253	Therefore, they could be used as indicator traits of hyperketonemia in a selection index aimed to reduce the susceptibility of dairy cows to metabolic disorders in early lactation.
32359995	8	113	theme	similar	1361:1367	arg1	variation					1377:1385	similar genetic variation	1361:1385	similar genetic variation across DIM	1361:1396	Blood BHB and NEFA showed similar genetic variation across DIM, with the highest heritability in the first 10 d after calving (0.31 ± 0.06 and 0.19 ± 0.05 for BHB and NEFA, respectively).
32359995	5	114	theme	=	1013:1013	arg1	n					1011:1011	n = 22,718	1011:1020	n = 22,718	1011:1020	The data set comprised the first test-day record within lactation and spectra of individual milk samples (n = 22,718) of 13,106 Holstein cows collected from 5 to 35 d in milk (DIM).
32359995	5	114	theme	=	1013:1013	arg1	samples					1002:1008	individual milk samples	986:1008	individual milk samples (n = 22,718) of 13,106 Holstein cows collected from 5 to 35 d in milk (DIM)	986:1084	The data set comprised the first test-day record within lactation and spectra of individual milk samples (n = 22,718) of 13,106 Holstein cows collected from 5 to 35 d in milk (DIM).
32359995	11	115	contain	have	1997:2000	arg1	BHB					1952:1954	blood BHB	1946:1954	blood BHB	1946:1954	Results of the current study suggest that blood BHB and NEFA predicted from milk MIR spectra have genetic variation that is potentially exploitable for breeding purposes.
32359995	11	115	contain	have	1997:2000	arg2	variation					2010:2018	genetic variation	2002:2018	genetic variation that is potentially exploitable for breeding purposes	2002:2072	Results of the current study suggest that blood BHB and NEFA predicted from milk MIR spectra have genetic variation that is potentially exploitable for breeding purposes.
32359995	11	115	contain	have	1997:2000	arg1	NEFA					1960:1963	NEFA	1960:1963	NEFA predicted from milk MIR spectra	1960:1995	Results of the current study suggest that blood BHB and NEFA predicted from milk MIR spectra have genetic variation that is potentially exploitable for breeding purposes.
32359995	3	116	theme	mid-infrared	538:549	arg1	spectroscopy					557:568	mid-infrared (MIR) spectroscopy	538:568	mid-infrared (MIR) spectroscopy	538:568	Recently, prediction models based on mid-infrared (MIR) spectroscopy have been developed to assess blood BHB and NEFA from routinely collected individual milk samples.
31958054	9	0	theme	phylogenetic	1267:1278	arg1	data					1280:1283	phenotypic, chemotaxonomic and phylogenetic data	1236:1283	phenotypic, chemotaxonomic and phylogenetic data	1236:1283	On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain XNB-1T is considered to represent a novel species of the genus Ornithinicoccus, for which the name Ornithinicoccus soli sp.
31958054	2	1	theme	coccoid-shaped	107:120	arg1	bacterium					122:130	A Gram-stain-positive, aerobic, non-motile and coccoid-shaped bacterium	60:130	A Gram-stain-positive, aerobic, non-motile and coccoid-shaped bacterium	60:130	A Gram-stain-positive, aerobic, non-motile and coccoid-shaped bacterium, designated XNB-1T, was isolated from farmland soil in Taian, Shandong province, China.
31958054	8	2	theme	Genome-based	959:970	arg1	analysis					972:979	Genome-based analysis	959:979	Genome-based analysis of average nucleotide identity of strain XNB-1T with O. hortensis HKI 0125T and O. halotolerans EGI 80423T	959:1086	Genome-based analysis of average nucleotide identity of strain XNB-1T with O. hortensis HKI 0125T and O. halotolerans EGI 80423T yielded values of 73.1 and 73.3 %, respectively, while the digital DNA-DNA hybridization values were 19.5 and 19.9 %, respectively.
31958054	9	3	dep	data	1280:1283	arg1	the					1223:1225	the	1223:1225	the	1223:1225	On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain XNB-1T is considered to represent a novel species of the genus Ornithinicoccus, for which the name Ornithinicoccus soli sp.
31958054	9	3	dep	data	1280:1283	arg1	basis					1227:1231	basis	1227:1231	basis	1227:1231	On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain XNB-1T is considered to represent a novel species of the genus Ornithinicoccus, for which the name Ornithinicoccus soli sp.
31958054	6	4	theme	predominant	599:609	arg1	MK-8					644:647	MK-8	644:647	MK-8	644:647	The predominant menaquinone of strain XNB-1T was MK-8(H4) and the DNA G+C content was 70.1 mol%.
31958054	6	4	theme	predominant	599:609	arg1	menaquinone					611:621	The predominant menaquinone	595:621	The predominant menaquinone of strain XNB-1T	595:638	The predominant menaquinone of strain XNB-1T was MK-8(H4) and the DNA G+C content was 70.1 mol%.
31958054	3	5	theme	predominant	279:289	arg1	iso-C15 					244:251	iso-C15 	244:251	iso-C15 	244:251	Strain XNB-1T contained iso-C15 : 0 and iso-C16 : 0 as the predominant fatty acids.
31958054	3	5	theme	predominant	279:289	arg1	acids					297:301	the predominant fatty acids	275:301	the predominant fatty acids	275:301	Strain XNB-1T contained iso-C15 : 0 and iso-C16 : 0 as the predominant fatty acids.
31958054	9	6	theme	Ornithinicoccus	1356:1370	arg1	species					1335:1341	a novel species	1327:1341	a novel species	1327:1341	On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain XNB-1T is considered to represent a novel species of the genus Ornithinicoccus, for which the name Ornithinicoccus soli sp.
31958054	7	7	theme	HKI	877:879	arg1	0125T					881:885	Ornithinicoccus hortensis HKI 0125T	851:885	Ornithinicoccus hortensis HKI 0125T	851:885	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain XNB-1T belonged to the genus Ornithinicoccus, and shared the highest similarity with Ornithinicoccus hortensis HKI 0125T (96.0 %), followed by Ornithinicoccus halotolerans EGI 80423T (95.5 %).
31958054	8	8	theme	halotolerans	1064:1075	arg1	80423T					1081:1086	O. hortensis HKI 0125T and O. halotolerans EGI 80423T	1034:1086	80423T	1081:1086	Genome-based analysis of average nucleotide identity of strain XNB-1T with O. hortensis HKI 0125T and O. halotolerans EGI 80423T yielded values of 73.1 and 73.3 %, respectively, while the digital DNA-DNA hybridization values were 19.5 and 19.9 %, respectively.
31958054	2	9	theme	non-motile	92:101	arg1	bacterium					122:130	A Gram-stain-positive, aerobic, non-motile and coccoid-shaped bacterium	60:130	A Gram-stain-positive, aerobic, non-motile and coccoid-shaped bacterium	60:130	A Gram-stain-positive, aerobic, non-motile and coccoid-shaped bacterium, designated XNB-1T, was isolated from farmland soil in Taian, Shandong province, China.
31958054	1	10	theme	farmland	45:52	arg1	soil					54:57	farmland soil	45:57	farmland soil	45:57	nov., isolated from farmland soil.
31958054	9	11	theme	novel	1329:1333	arg1	species					1335:1341	a novel species	1327:1341	a novel species	1327:1341	On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain XNB-1T is considered to represent a novel species of the genus Ornithinicoccus, for which the name Ornithinicoccus soli sp.
31958054	2	12	from	soil	179:182	arg1	Shandong					194:201	Shandong	194:201	Shandong	194:201	A Gram-stain-positive, aerobic, non-motile and coccoid-shaped bacterium, designated XNB-1T, was isolated from farmland soil in Taian, Shandong province, China.
31958054	2	12	from	soil	179:182	arg1	China					213:217	China	213:217	China	213:217	A Gram-stain-positive, aerobic, non-motile and coccoid-shaped bacterium, designated XNB-1T, was isolated from farmland soil in Taian, Shandong province, China.
31958054	5	13	theme	unidentified	567:578	arg1	phospholipids					580:592	three unidentified phospholipids	561:592	three unidentified phospholipids	561:592	The polar lipid profile of strain XNB-1T consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phosphoglycolipid and three unidentified phospholipids.
31958054	6	14	theme	G+C	665:667	arg1	content					669:675	the DNA G+C content	657:675	the DNA G+C content	657:675	The predominant menaquinone of strain XNB-1T was MK-8(H4) and the DNA G+C content was 70.1 mol%.
31958054	6	14	theme	G+C	665:667	arg1	%					689:689	70.1 mol%	681:689	70.1 mol%	681:689	The predominant menaquinone of strain XNB-1T was MK-8(H4) and the DNA G+C content was 70.1 mol%.
31958054	4	15	dep	l-Orn←Gly	400:408	arg1	2					413:413	2	413:413	2	413:413	The diagnostic diamino acid of the peptidoglycan was ornithine, and the interpeptide bridge was l-Orn←Gly(1, 2)←d-Glu.
31958054	11	16	theme	2019099T=KCTC	1472:1484	arg1	XNB-1T					1454:1459	XNB-1T	1454:1459	XNB-1T (=CCTCC AB 2019099T=KCTC 49259T)	1454:1492	The type strain is XNB-1T (=CCTCC AB 2019099T=KCTC 49259T).
31958054	11	16	theme	2019099T=KCTC	1472:1484	arg1	49259T					1486:1491	=CCTCC AB 2019099T=KCTC 49259T	1462:1491	=CCTCC AB 2019099T=KCTC 49259T	1462:1491	The type strain is XNB-1T (=CCTCC AB 2019099T=KCTC 49259T).
31958054	8	17	theme	nucleotide	992:1001	arg1	identity					1003:1010	average nucleotide identity	984:1010	average nucleotide identity of strain XNB-1T with O. hortensis HKI 0125T and O. halotolerans EGI 80423T	984:1086	Genome-based analysis of average nucleotide identity of strain XNB-1T with O. hortensis HKI 0125T and O. halotolerans EGI 80423T yielded values of 73.1 and 73.3 %, respectively, while the digital DNA-DNA hybridization values were 19.5 and 19.9 %, respectively.
31958054	5	18	theme	polar	427:431	arg1	profile					439:445	The polar lipid profile	423:445	The polar lipid profile of strain XNB-1T	423:462	The polar lipid profile of strain XNB-1T consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phosphoglycolipid and three unidentified phospholipids.
31958054	8	19	theme	DNA-DNA	1155:1161	arg1	hybridization					1163:1175	the digital DNA-DNA hybridization	1143:1175	the digital DNA-DNA hybridization values	1143:1182	Genome-based analysis of average nucleotide identity of strain XNB-1T with O. hortensis HKI 0125T and O. halotolerans EGI 80423T yielded values of 73.1 and 73.3 %, respectively, while the digital DNA-DNA hybridization values were 19.5 and 19.9 %, respectively.
31958054	8	20	theme	average	984:990	arg1	identity					1003:1010	average nucleotide identity	984:1010	average nucleotide identity of strain XNB-1T with O. hortensis HKI 0125T and O. halotolerans EGI 80423T	984:1086	Genome-based analysis of average nucleotide identity of strain XNB-1T with O. hortensis HKI 0125T and O. halotolerans EGI 80423T yielded values of 73.1 and 73.3 %, respectively, while the digital DNA-DNA hybridization values were 19.5 and 19.9 %, respectively.
31958054	7	21	dep	shared	816:821	arg1	%					893:893	96.0 %	888:893	96.0 %	888:893	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain XNB-1T belonged to the genus Ornithinicoccus, and shared the highest similarity with Ornithinicoccus hortensis HKI 0125T (96.0 %), followed by Ornithinicoccus halotolerans EGI 80423T (95.5 %).
31958054	9	22	theme	Ornithinicoccus	1392:1406	arg1	sp					1413:1414	the name Ornithinicoccus soli sp	1383:1414	the name Ornithinicoccus soli sp	1383:1414	On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain XNB-1T is considered to represent a novel species of the genus Ornithinicoccus, for which the name Ornithinicoccus soli sp.
31958054	5	23	theme	lipid	433:437	arg1	profile					439:445	The polar lipid profile	423:445	The polar lipid profile of strain XNB-1T	423:462	The polar lipid profile of strain XNB-1T consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phosphoglycolipid and three unidentified phospholipids.
31958054	8	24	theme	digital	1147:1153	arg1	hybridization					1163:1175	the digital DNA-DNA hybridization	1143:1175	the digital DNA-DNA hybridization values	1143:1182	Genome-based analysis of average nucleotide identity of strain XNB-1T with O. hortensis HKI 0125T and O. halotolerans EGI 80423T yielded values of 73.1 and 73.3 %, respectively, while the digital DNA-DNA hybridization values were 19.5 and 19.9 %, respectively.
31958054	0	25	theme	soli	16:19	arg1	sp					21:22	Ornithinicoccus soli sp	0:22	Ornithinicoccus soli sp.	0:23	Ornithinicoccus soli sp.
31958054	8	26	theme	EGI	1077:1079	arg1	80423T					1081:1086	O. hortensis HKI 0125T and O. halotolerans EGI 80423T	1034:1086	80423T	1081:1086	Genome-based analysis of average nucleotide identity of strain XNB-1T with O. hortensis HKI 0125T and O. halotolerans EGI 80423T yielded values of 73.1 and 73.3 %, respectively, while the digital DNA-DNA hybridization values were 19.5 and 19.9 %, respectively.
31958054	3	27	theme	fatty	291:295	arg1	iso-C15 					244:251	iso-C15 	244:251	iso-C15 	244:251	Strain XNB-1T contained iso-C15 : 0 and iso-C16 : 0 as the predominant fatty acids.
31958054	3	27	theme	fatty	291:295	arg1	acids					297:301	the predominant fatty acids	275:301	the predominant fatty acids	275:301	Strain XNB-1T contained iso-C15 : 0 and iso-C16 : 0 as the predominant fatty acids.
31958054	7	28	theme	16S	723:725	arg1	sequences					737:745	16S rRNA gene sequences	723:745	16S rRNA gene sequences	723:745	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain XNB-1T belonged to the genus Ornithinicoccus, and shared the highest similarity with Ornithinicoccus hortensis HKI 0125T (96.0 %), followed by Ornithinicoccus halotolerans EGI 80423T (95.5 %).
31958054	9	29	theme	genus	1350:1354	arg1	Ornithinicoccus					1356:1370	the genus Ornithinicoccus	1346:1370	the genus Ornithinicoccus	1346:1370	On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain XNB-1T is considered to represent a novel species of the genus Ornithinicoccus, for which the name Ornithinicoccus soli sp.
31958054	0	30	theme	Ornithinicoccus	0:14	arg1	sp					21:22	Ornithinicoccus soli sp	0:22	Ornithinicoccus soli sp.	0:23	Ornithinicoccus soli sp.
31958054	9	31	theme	phenotypic	1236:1245	arg1	data					1280:1283	phenotypic, chemotaxonomic and phylogenetic data	1236:1283	phenotypic, chemotaxonomic and phylogenetic data	1236:1283	On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain XNB-1T is considered to represent a novel species of the genus Ornithinicoccus, for which the name Ornithinicoccus soli sp.
31958054	3	32	theme	Strain	220:225	arg1	XNB-1T					227:232	Strain XNB-1T	220:232	Strain XNB-1T	220:232	Strain XNB-1T contained iso-C15 : 0 and iso-C16 : 0 as the predominant fatty acids.
31958054	4	33	theme	peptidoglycan	339:351	arg1	ornithine					357:365	ornithine	357:365	ornithine	357:365	The diagnostic diamino acid of the peptidoglycan was ornithine, and the interpeptide bridge was l-Orn←Gly(1, 2)←d-Glu.
31958054	4	33	theme	peptidoglycan	339:351	arg1	acid					327:330	The diagnostic diamino acid	304:330	The diagnostic diamino acid of the peptidoglycan	304:351	The diagnostic diamino acid of the peptidoglycan was ornithine, and the interpeptide bridge was l-Orn←Gly(1, 2)←d-Glu.
31958054	11	34	theme	=CCTCC AB	1462:1470	arg1	XNB-1T					1454:1459	XNB-1T	1454:1459	XNB-1T (=CCTCC AB 2019099T=KCTC 49259T)	1454:1492	The type strain is XNB-1T (=CCTCC AB 2019099T=KCTC 49259T).
31958054	11	34	theme	=CCTCC AB	1462:1470	arg1	49259T					1486:1491	=CCTCC AB 2019099T=KCTC 49259T	1462:1491	=CCTCC AB 2019099T=KCTC 49259T	1462:1491	The type strain is XNB-1T (=CCTCC AB 2019099T=KCTC 49259T).
31958054	7	35	dep	Ornithinicoccus	909:923	arg1	halotolerans					925:936	halotolerans	925:936	halotolerans	925:936	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain XNB-1T belonged to the genus Ornithinicoccus, and shared the highest similarity with Ornithinicoccus hortensis HKI 0125T (96.0 %), followed by Ornithinicoccus halotolerans EGI 80423T (95.5 %).
31958054	8	36	theme	hybridization	1163:1175	arg1	values					1177:1182	the digital DNA-DNA hybridization values	1143:1182	the digital DNA-DNA hybridization values	1143:1182	Genome-based analysis of average nucleotide identity of strain XNB-1T with O. hortensis HKI 0125T and O. halotolerans EGI 80423T yielded values of 73.1 and 73.3 %, respectively, while the digital DNA-DNA hybridization values were 19.5 and 19.9 %, respectively.
31958054	5	37	theme	unidentified	526:537	arg1	phosphoglycolipid					539:555	an unidentified phosphoglycolipid	523:555	an unidentified phosphoglycolipid	523:555	The polar lipid profile of strain XNB-1T consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phosphoglycolipid and three unidentified phospholipids.
31958054	8	38	theme	XNB-1T	1022:1027	arg1	identity					1003:1010	average nucleotide identity	984:1010	average nucleotide identity of strain XNB-1T with O. hortensis HKI 0125T and O. halotolerans EGI 80423T	984:1086	Genome-based analysis of average nucleotide identity of strain XNB-1T with O. hortensis HKI 0125T and O. halotolerans EGI 80423T yielded values of 73.1 and 73.3 %, respectively, while the digital DNA-DNA hybridization values were 19.5 and 19.9 %, respectively.
31958054	8	39	with	identity	1003:1010	arg1	80423T					1081:1086	O. hortensis HKI 0125T and O. halotolerans EGI 80423T	1034:1086	80423T	1081:1086	Genome-based analysis of average nucleotide identity of strain XNB-1T with O. hortensis HKI 0125T and O. halotolerans EGI 80423T yielded values of 73.1 and 73.3 %, respectively, while the digital DNA-DNA hybridization values were 19.5 and 19.9 %, respectively.
31958054	8	39	with	identity	1003:1010	arg1	0125T					1051:1055	0125T	1051:1055	0125T	1051:1055	Genome-based analysis of average nucleotide identity of strain XNB-1T with O. hortensis HKI 0125T and O. halotolerans EGI 80423T yielded values of 73.1 and 73.3 %, respectively, while the digital DNA-DNA hybridization values were 19.5 and 19.9 %, respectively.
31958054	6	40	theme	70.1 mol	681:688	arg1	content					669:675	the DNA G+C content	657:675	the DNA G+C content	657:675	The predominant menaquinone of strain XNB-1T was MK-8(H4) and the DNA G+C content was 70.1 mol%.
31958054	6	40	theme	70.1 mol	681:688	arg1	%					689:689	70.1 mol%	681:689	70.1 mol%	681:689	The predominant menaquinone of strain XNB-1T was MK-8(H4) and the DNA G+C content was 70.1 mol%.
31958054	9	41	theme	chemotaxonomic	1248:1261	arg1	data					1280:1283	phenotypic, chemotaxonomic and phylogenetic data	1236:1283	phenotypic, chemotaxonomic and phylogenetic data	1236:1283	On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain XNB-1T is considered to represent a novel species of the genus Ornithinicoccus, for which the name Ornithinicoccus soli sp.
31958054	7	42	theme	Ornithinicoccus	909:923	arg1	80423T					942:947	Ornithinicoccus halotolerans EGI 80423T	909:947	Ornithinicoccus halotolerans EGI 80423T (95.5 %)	909:956	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain XNB-1T belonged to the genus Ornithinicoccus, and shared the highest similarity with Ornithinicoccus hortensis HKI 0125T (96.0 %), followed by Ornithinicoccus halotolerans EGI 80423T (95.5 %).
31958054	7	42	theme	Ornithinicoccus	909:923	arg1	%					955:955	95.5 %	950:955	95.5 %	950:955	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain XNB-1T belonged to the genus Ornithinicoccus, and shared the highest similarity with Ornithinicoccus hortensis HKI 0125T (96.0 %), followed by Ornithinicoccus halotolerans EGI 80423T (95.5 %).
31958054	2	43	theme	farmland	170:177	arg1	soil					179:182	farmland soil	170:182	farmland soil in Taian, Shandong province, China	170:217	A Gram-stain-positive, aerobic, non-motile and coccoid-shaped bacterium, designated XNB-1T, was isolated from farmland soil in Taian, Shandong province, China.
31958054	6	44	theme	DNA	661:663	arg1	content					669:675	the DNA G+C content	657:675	the DNA G+C content	657:675	The predominant menaquinone of strain XNB-1T was MK-8(H4) and the DNA G+C content was 70.1 mol%.
31958054	6	44	theme	DNA	661:663	arg1	%					689:689	70.1 mol%	681:689	70.1 mol%	681:689	The predominant menaquinone of strain XNB-1T was MK-8(H4) and the DNA G+C content was 70.1 mol%.
31958054	8	45	theme	strain	1015:1020	arg1	XNB-1T					1022:1027	strain XNB-1T	1015:1027	strain XNB-1T	1015:1027	Genome-based analysis of average nucleotide identity of strain XNB-1T with O. hortensis HKI 0125T and O. halotolerans EGI 80423T yielded values of 73.1 and 73.3 %, respectively, while the digital DNA-DNA hybridization values were 19.5 and 19.9 %, respectively.
31958054	8	46	theme	%	1120:1120	arg1	values					1096:1101	values	1096:1101	values of 73.1 and 73.3 %	1096:1120	Genome-based analysis of average nucleotide identity of strain XNB-1T with O. hortensis HKI 0125T and O. halotolerans EGI 80423T yielded values of 73.1 and 73.3 %, respectively, while the digital DNA-DNA hybridization values were 19.5 and 19.9 %, respectively.
31958054	7	47	theme	rRNA	727:730	arg1	sequences					737:745	16S rRNA gene sequences	723:745	16S rRNA gene sequences	723:745	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain XNB-1T belonged to the genus Ornithinicoccus, and shared the highest similarity with Ornithinicoccus hortensis HKI 0125T (96.0 %), followed by Ornithinicoccus halotolerans EGI 80423T (95.5 %).
31958054	9	48	theme	strain	1286:1291	arg1	XNB-1T					1293:1298	strain XNB-1T	1286:1298	strain XNB-1T	1286:1298	On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain XNB-1T is considered to represent a novel species of the genus Ornithinicoccus, for which the name Ornithinicoccus soli sp.
31958054	2	49	attach	isolated	156:163	arg2	bacterium					122:130	A Gram-stain-positive, aerobic, non-motile and coccoid-shaped bacterium	60:130	A Gram-stain-positive, aerobic, non-motile and coccoid-shaped bacterium	60:130	A Gram-stain-positive, aerobic, non-motile and coccoid-shaped bacterium, designated XNB-1T, was isolated from farmland soil in Taian, Shandong province, China.
31958054	2	49	attach	isolated	156:163	arg1	soil					179:182	farmland soil	170:182	farmland soil in Taian, Shandong province, China	170:217	A Gram-stain-positive, aerobic, non-motile and coccoid-shaped bacterium, designated XNB-1T, was isolated from farmland soil in Taian, Shandong province, China.
31958054	2	50	theme	aerobic	83:89	arg1	bacterium					122:130	A Gram-stain-positive, aerobic, non-motile and coccoid-shaped bacterium	60:130	A Gram-stain-positive, aerobic, non-motile and coccoid-shaped bacterium	60:130	A Gram-stain-positive, aerobic, non-motile and coccoid-shaped bacterium, designated XNB-1T, was isolated from farmland soil in Taian, Shandong province, China.
31958054	7	51	theme	strain	759:764	arg1	XNB-1T					766:771	strain XNB-1T	759:771	strain XNB-1T	759:771	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain XNB-1T belonged to the genus Ornithinicoccus, and shared the highest similarity with Ornithinicoccus hortensis HKI 0125T (96.0 %), followed by Ornithinicoccus halotolerans EGI 80423T (95.5 %).
31958054	11	52	theme	type	1439:1442	arg1	XNB-1T					1454:1459	XNB-1T	1454:1459	XNB-1T (=CCTCC AB 2019099T=KCTC 49259T)	1454:1492	The type strain is XNB-1T (=CCTCC AB 2019099T=KCTC 49259T).
31958054	11	52	theme	type	1439:1442	arg1	strain					1444:1449	The type strain	1435:1449	The type strain	1435:1449	The type strain is XNB-1T (=CCTCC AB 2019099T=KCTC 49259T).
31958054	9	53	theme	name	1387:1390	arg1	sp					1413:1414	the name Ornithinicoccus soli sp	1383:1414	the name Ornithinicoccus soli sp	1383:1414	On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain XNB-1T is considered to represent a novel species of the genus Ornithinicoccus, for which the name Ornithinicoccus soli sp.
31958054	4	54	theme	interpeptide	376:387	arg1	bridge					389:394	the interpeptide bridge	372:394	the interpeptide bridge	372:394	The diagnostic diamino acid of the peptidoglycan was ornithine, and the interpeptide bridge was l-Orn←Gly(1, 2)←d-Glu.
31958054	4	54	theme	interpeptide	376:387	arg1	←d-Glu					415:420	←d-Glu	415:420	←d-Glu	415:420	The diagnostic diamino acid of the peptidoglycan was ornithine, and the interpeptide bridge was l-Orn←Gly(1, 2)←d-Glu.
31958054	4	55	theme	diamino	319:325	arg1	ornithine					357:365	ornithine	357:365	ornithine	357:365	The diagnostic diamino acid of the peptidoglycan was ornithine, and the interpeptide bridge was l-Orn←Gly(1, 2)←d-Glu.
31958054	4	55	theme	diamino	319:325	arg1	acid					327:330	The diagnostic diamino acid	304:330	The diagnostic diamino acid of the peptidoglycan	304:351	The diagnostic diamino acid of the peptidoglycan was ornithine, and the interpeptide bridge was l-Orn←Gly(1, 2)←d-Glu.
31958054	6	56	theme	strain	626:631	arg1	XNB-1T					633:638	strain XNB-1T	626:638	strain XNB-1T	626:638	The predominant menaquinone of strain XNB-1T was MK-8(H4) and the DNA G+C content was 70.1 mol%.
31958054	7	57	theme	hortensis	867:875	arg1	0125T					881:885	Ornithinicoccus hortensis HKI 0125T	851:885	Ornithinicoccus hortensis HKI 0125T	851:885	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain XNB-1T belonged to the genus Ornithinicoccus, and shared the highest similarity with Ornithinicoccus hortensis HKI 0125T (96.0 %), followed by Ornithinicoccus halotolerans EGI 80423T (95.5 %).
31958054	2	58	theme	Gram-stain-positive	62:80	arg1	bacterium					122:130	A Gram-stain-positive, aerobic, non-motile and coccoid-shaped bacterium	60:130	A Gram-stain-positive, aerobic, non-motile and coccoid-shaped bacterium	60:130	A Gram-stain-positive, aerobic, non-motile and coccoid-shaped bacterium, designated XNB-1T, was isolated from farmland soil in Taian, Shandong province, China.
31958054	4	59	theme	diagnostic	308:317	arg1	ornithine					357:365	ornithine	357:365	ornithine	357:365	The diagnostic diamino acid of the peptidoglycan was ornithine, and the interpeptide bridge was l-Orn←Gly(1, 2)←d-Glu.
31958054	4	59	theme	diagnostic	308:317	arg1	acid					327:330	The diagnostic diamino acid	304:330	The diagnostic diamino acid of the peptidoglycan	304:351	The diagnostic diamino acid of the peptidoglycan was ornithine, and the interpeptide bridge was l-Orn←Gly(1, 2)←d-Glu.
31958054	9	60	theme	soli	1408:1411	arg1	sp					1413:1414	the name Ornithinicoccus soli sp	1383:1414	the name Ornithinicoccus soli sp	1383:1414	On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain XNB-1T is considered to represent a novel species of the genus Ornithinicoccus, for which the name Ornithinicoccus soli sp.
31958054	6	61	theme	XNB-1T	633:638	arg1	MK-8					644:647	MK-8	644:647	MK-8	644:647	The predominant menaquinone of strain XNB-1T was MK-8(H4) and the DNA G+C content was 70.1 mol%.
31958054	6	61	theme	XNB-1T	633:638	arg1	menaquinone					611:621	The predominant menaquinone	595:621	The predominant menaquinone of strain XNB-1T	595:638	The predominant menaquinone of strain XNB-1T was MK-8(H4) and the DNA G+C content was 70.1 mol%.
31958054	7	62	theme	EGI	938:940	arg1	80423T					942:947	Ornithinicoccus halotolerans EGI 80423T	909:947	Ornithinicoccus halotolerans EGI 80423T (95.5 %)	909:956	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain XNB-1T belonged to the genus Ornithinicoccus, and shared the highest similarity with Ornithinicoccus hortensis HKI 0125T (96.0 %), followed by Ornithinicoccus halotolerans EGI 80423T (95.5 %).
31958054	7	62	theme	EGI	938:940	arg1	%					955:955	95.5 %	950:955	95.5 %	950:955	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain XNB-1T belonged to the genus Ornithinicoccus, and shared the highest similarity with Ornithinicoccus hortensis HKI 0125T (96.0 %), followed by Ornithinicoccus halotolerans EGI 80423T (95.5 %).
31958054	3	63	contain	contained	234:242	arg2	iso-C15 					244:251	iso-C15 	244:251	iso-C15 	244:251	Strain XNB-1T contained iso-C15 : 0 and iso-C16 : 0 as the predominant fatty acids.
31958054	3	63	contain	contained	234:242	arg1	XNB-1T					227:232	Strain XNB-1T	220:232	Strain XNB-1T	220:232	Strain XNB-1T contained iso-C15 : 0 and iso-C16 : 0 as the predominant fatty acids.
31958054	3	63	contain	contained	234:242	arg2	acids					297:301	the predominant fatty acids	275:301	the predominant fatty acids	275:301	Strain XNB-1T contained iso-C15 : 0 and iso-C16 : 0 as the predominant fatty acids.
31958054	7	64	theme	genus	789:793	arg1	Ornithinicoccus					795:809	the genus Ornithinicoccus	785:809	the genus Ornithinicoccus	785:809	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain XNB-1T belonged to the genus Ornithinicoccus, and shared the highest similarity with Ornithinicoccus hortensis HKI 0125T (96.0 %), followed by Ornithinicoccus halotolerans EGI 80423T (95.5 %).
31958054	3	65	dep	iso-C15 	244:251	arg1	 0					253:254	 0	253:254	 0	253:254	Strain XNB-1T contained iso-C15 : 0 and iso-C16 : 0 as the predominant fatty acids.
31958054	3	65	dep	iso-C15 	244:251	arg1	 0					269:270	 0	269:270	 0	269:270	Strain XNB-1T contained iso-C15 : 0 and iso-C16 : 0 as the predominant fatty acids.
31958054	3	65	dep	iso-C15 	244:251	arg1	iso-C16 					260:267	iso-C16 	260:267	iso-C16 	260:267	Strain XNB-1T contained iso-C15 : 0 and iso-C16 : 0 as the predominant fatty acids.
31958054	8	66	theme	identity	1003:1010	arg1	analysis					972:979	Genome-based analysis	959:979	Genome-based analysis of average nucleotide identity of strain XNB-1T with O. hortensis HKI 0125T and O. halotolerans EGI 80423T	959:1086	Genome-based analysis of average nucleotide identity of strain XNB-1T with O. hortensis HKI 0125T and O. halotolerans EGI 80423T yielded values of 73.1 and 73.3 %, respectively, while the digital DNA-DNA hybridization values were 19.5 and 19.9 %, respectively.
31958054	5	67	theme	strain	450:455	arg1	XNB-1T					457:462	strain XNB-1T	450:462	strain XNB-1T	450:462	The polar lipid profile of strain XNB-1T consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phosphoglycolipid and three unidentified phospholipids.
31958054	7	68	theme	Ornithinicoccus	851:865	arg1	0125T					881:885	Ornithinicoccus hortensis HKI 0125T	851:885	Ornithinicoccus hortensis HKI 0125T	851:885	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain XNB-1T belonged to the genus Ornithinicoccus, and shared the highest similarity with Ornithinicoccus hortensis HKI 0125T (96.0 %), followed by Ornithinicoccus halotolerans EGI 80423T (95.5 %).
31958054	8	69	theme	O.	1061:1062	arg1	80423T					1081:1086	O. hortensis HKI 0125T and O. halotolerans EGI 80423T	1034:1086	80423T	1081:1086	Genome-based analysis of average nucleotide identity of strain XNB-1T with O. hortensis HKI 0125T and O. halotolerans EGI 80423T yielded values of 73.1 and 73.3 %, respectively, while the digital DNA-DNA hybridization values were 19.5 and 19.9 %, respectively.
31958054	7	70	theme	Phylogenetic	692:703	arg1	analysis					705:712	Phylogenetic analysis	692:712	Phylogenetic analysis based on 16S rRNA gene sequences	692:745	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain XNB-1T belonged to the genus Ornithinicoccus, and shared the highest similarity with Ornithinicoccus hortensis HKI 0125T (96.0 %), followed by Ornithinicoccus halotolerans EGI 80423T (95.5 %).
31958054	5	71	theme	XNB-1T	457:462	arg1	profile					439:445	The polar lipid profile	423:445	The polar lipid profile of strain XNB-1T	423:462	The polar lipid profile of strain XNB-1T consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified phosphoglycolipid and three unidentified phospholipids.
31958054	7	72	theme	highest	827:833	arg1	similarity					835:844	the highest similarity	823:844	the highest similarity	823:844	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain XNB-1T belonged to the genus Ornithinicoccus, and shared the highest similarity with Ornithinicoccus hortensis HKI 0125T (96.0 %), followed by Ornithinicoccus halotolerans EGI 80423T (95.5 %).
31958054	7	73	theme	gene	732:735	arg1	sequences					737:745	16S rRNA gene sequences	723:745	16S rRNA gene sequences	723:745	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain XNB-1T belonged to the genus Ornithinicoccus, and shared the highest similarity with Ornithinicoccus hortensis HKI 0125T (96.0 %), followed by Ornithinicoccus halotolerans EGI 80423T (95.5 %).
32928871	5	0	theme	GLP-1	717:721	arg1	secretion					723:731	GLP-1 secretion	717:731	GLP-1 secretion	717:731	In mice fed a Western (high-fat/high-sucrose) diet for 16 weeks, GLP-1 secretion was markedly increased but arrhythmic over the 24-h day, whereas levels of the other incretin, glucose-dependent insulinotropic polypeptide, were not as profoundly affected.
32928871	0	1	from	Secretion	16:24	arg1	Mice					29:32	Mice	29:32	Mice	29:32	Circadian GLP-1 Secretion in Mice Is Dependent on the Intestinal Microbiome for Maintenance of Diurnal Metabolic Homeostasis.
32928871	7	2	theme	transcriptome	1078:1090	arg1	Analysis					1047:1054	Analysis	1047:1054	Analysis of the primary L-cell transcriptome, as well as of the intestinal microbiome,	1047:1132	Analysis of the primary L-cell transcriptome, as well as of the intestinal microbiome, also demonstrated time-of-day- and diet-dependent changes paralleling GLP-1 secretion.
32928871	8	3	from	studies	1230:1236	arg1	mice					1296:1299	germ-free mice	1286:1299	germ-free mice with and without fecal microbial transfer	1286:1341	Finally, studies in antibiotic-induced microbial depleted and in germ-free mice with and without fecal microbial transfer, provided evidence for a role of the microbiome in diurnal GLP-1 release.
32928871	8	3	from	studies	1230:1236	arg1	microbial					1260:1268	microbial	1260:1268	microbial	1260:1268	Finally, studies in antibiotic-induced microbial depleted and in germ-free mice with and without fecal microbial transfer, provided evidence for a role of the microbiome in diurnal GLP-1 release.
32928871	2	4	theme	period	322:327	arg1	onset					301:305	the onset	297:305	the onset of the feeding period	297:327	GLP-1 also exhibits a circadian rhythm, with highest release at the onset of the feeding period.
32928871	5	5	theme	Western	666:672	arg1	diet					698:701	a Western (high-fat/high-sucrose) diet	664:701	a Western (high-fat/high-sucrose) diet for 16 weeks	664:714	In mice fed a Western (high-fat/high-sucrose) diet for 16 weeks, GLP-1 secretion was markedly increased but arrhythmic over the 24-h day, whereas levels of the other incretin, glucose-dependent insulinotropic polypeptide, were not as profoundly affected.
32928871	7	6	theme	L-cell	1071:1076	arg1	transcriptome					1078:1090	the primary L-cell transcriptome	1059:1090	the primary L-cell transcriptome	1059:1090	Analysis of the primary L-cell transcriptome, as well as of the intestinal microbiome, also demonstrated time-of-day- and diet-dependent changes paralleling GLP-1 secretion.
32928871	2	7	from	onset	301:305	arg1	release					286:292	highest release	278:292	highest release at the onset of the feeding period	278:327	GLP-1 also exhibits a circadian rhythm, with highest release at the onset of the feeding period.
32928871	2	8	theme	feeding	314:320	arg1	period					322:327	the feeding period	310:327	the feeding period	310:327	GLP-1 also exhibits a circadian rhythm, with highest release at the onset of the feeding period.
32928871	4	9	from	release	572:578	arg1	mice					646:649	normal male and female mice	623:649	normal male and female mice	623:649	The circadian pattern of GLP-1 release was found to be dependent on the oral route of glucose administration and was necessary for the rhythmic release of insulin and diurnal glycemic control in normal male and female mice.
32928871	9	10	theme	GLP-1	1504:1508	arg1	secretion					1510:1518	microbiome-dependent circadian GLP-1 secretion	1473:1518	microbiome-dependent circadian GLP-1 secretion	1473:1518	In combination, these findings establish a key role for microbiome-dependent circadian GLP-1 secretion in the maintenance of 24-h metabolic homeostasis.
32928871	7	11	theme	primary	1063:1069	arg1	transcriptome					1078:1090	the primary L-cell transcriptome	1059:1090	the primary L-cell transcriptome	1059:1090	Analysis of the primary L-cell transcriptome, as well as of the intestinal microbiome, also demonstrated time-of-day- and diet-dependent changes paralleling GLP-1 secretion.
32928871	5	12	theme	glucose-dependent	828:844	arg1	polypeptide					861:871	glucose-dependent insulinotropic polypeptide	828:871	glucose-dependent insulinotropic polypeptide	828:871	In mice fed a Western (high-fat/high-sucrose) diet for 16 weeks, GLP-1 secretion was markedly increased but arrhythmic over the 24-h day, whereas levels of the other incretin, glucose-dependent insulinotropic polypeptide, were not as profoundly affected.
32928871	5	12	theme	glucose-dependent	828:844	arg1	incretin					818:825	the other incretin	808:825	the other incretin	808:825	In mice fed a Western (high-fat/high-sucrose) diet for 16 weeks, GLP-1 secretion was markedly increased but arrhythmic over the 24-h day, whereas levels of the other incretin, glucose-dependent insulinotropic polypeptide, were not as profoundly affected.
32928871	9	13	theme	microbiome-dependent	1473:1492	arg1	secretion					1510:1518	microbiome-dependent circadian GLP-1 secretion	1473:1518	microbiome-dependent circadian GLP-1 secretion	1473:1518	In combination, these findings establish a key role for microbiome-dependent circadian GLP-1 secretion in the maintenance of 24-h metabolic homeostasis.
32928871	6	14	theme	GLP-1	935:939	arg1	secretion					941:949	GLP-1 secretion	935:949	GLP-1 secretion	935:949	Furthermore, the changes in GLP-1 secretion were shown to be essential for the maintenance of normoglycemia in this obesogenic environment.
32928871	5	15	theme	insulinotropic	846:859	arg1	polypeptide					861:871	glucose-dependent insulinotropic polypeptide	828:871	glucose-dependent insulinotropic polypeptide	828:871	In mice fed a Western (high-fat/high-sucrose) diet for 16 weeks, GLP-1 secretion was markedly increased but arrhythmic over the 24-h day, whereas levels of the other incretin, glucose-dependent insulinotropic polypeptide, were not as profoundly affected.
32928871	5	15	theme	insulinotropic	846:859	arg1	incretin					818:825	the other incretin	808:825	the other incretin	808:825	In mice fed a Western (high-fat/high-sucrose) diet for 16 weeks, GLP-1 secretion was markedly increased but arrhythmic over the 24-h day, whereas levels of the other incretin, glucose-dependent insulinotropic polypeptide, were not as profoundly affected.
32928871	4	16	theme	rhythmic	563:570	arg1	release					572:578	the rhythmic release	559:578	the rhythmic release of insulin and diurnal glycemic control in normal male and female mice	559:649	The circadian pattern of GLP-1 release was found to be dependent on the oral route of glucose administration and was necessary for the rhythmic release of insulin and diurnal glycemic control in normal male and female mice.
32928871	0	17	theme	Metabolic	103:111	arg1	Homeostasis					113:123	Diurnal Metabolic Homeostasis	95:123	Diurnal Metabolic Homeostasis	95:123	Circadian GLP-1 Secretion in Mice Is Dependent on the Intestinal Microbiome for Maintenance of Diurnal Metabolic Homeostasis.
32928871	4	18	theme	release	459:465	arg1	pattern					442:448	The circadian pattern	428:448	The circadian pattern of GLP-1 release	428:465	The circadian pattern of GLP-1 release was found to be dependent on the oral route of glucose administration and was necessary for the rhythmic release of insulin and diurnal glycemic control in normal male and female mice.
32928871	4	18	theme	release	459:465	arg1	dependent					483:491	dependent	483:491	dependent	483:491	The circadian pattern of GLP-1 release was found to be dependent on the oral route of glucose administration and was necessary for the rhythmic release of insulin and diurnal glycemic control in normal male and female mice.
32928871	4	18	theme	release	459:465	arg1	necessary					545:553	necessary	545:553	necessary	545:553	The circadian pattern of GLP-1 release was found to be dependent on the oral route of glucose administration and was necessary for the rhythmic release of insulin and diurnal glycemic control in normal male and female mice.
32928871	7	19	theme	intestinal	1111:1120	arg1	microbiome					1122:1131	the intestinal microbiome	1107:1131	the intestinal microbiome	1107:1131	Analysis of the primary L-cell transcriptome, as well as of the intestinal microbiome, also demonstrated time-of-day- and diet-dependent changes paralleling GLP-1 secretion.
32928871	2	20	theme	circadian	255:263	arg1	rhythm					265:270	a circadian rhythm	253:270	a circadian rhythm	253:270	GLP-1 also exhibits a circadian rhythm, with highest release at the onset of the feeding period.
32928871	0	21	theme	Diurnal	95:101	arg1	Homeostasis					113:123	Diurnal Metabolic Homeostasis	95:123	Diurnal Metabolic Homeostasis	95:123	Circadian GLP-1 Secretion in Mice Is Dependent on the Intestinal Microbiome for Maintenance of Diurnal Metabolic Homeostasis.
32928871	6	22	from	maintenance	986:996	arg1	environment					1034:1044	this obesogenic environment	1018:1044	this obesogenic environment	1018:1044	Furthermore, the changes in GLP-1 secretion were shown to be essential for the maintenance of normoglycemia in this obesogenic environment.
32928871	7	23	theme	time-of-day-	1152:1163	arg1	changes					1184:1190	time-of-day- and diet-dependent changes	1152:1190	time-of-day- and diet-dependent changes paralleling GLP-1 secretion	1152:1218	Analysis of the primary L-cell transcriptome, as well as of the intestinal microbiome, also demonstrated time-of-day- and diet-dependent changes paralleling GLP-1 secretion.
32928871	4	24	theme	male	630:633	arg1	mice					646:649	normal male and female mice	623:649	normal male and female mice	623:649	The circadian pattern of GLP-1 release was found to be dependent on the oral route of glucose administration and was necessary for the rhythmic release of insulin and diurnal glycemic control in normal male and female mice.
32928871	0	25	theme	GLP-1	10:14	arg1	Secretion					16:24	Circadian GLP-1 Secretion	0:24	Circadian GLP-1 Secretion in Mice	0:32	Circadian GLP-1 Secretion in Mice Is Dependent on the Intestinal Microbiome for Maintenance of Diurnal Metabolic Homeostasis.
32928871	9	26	theme	metabolic	1547:1555	arg1	homeostasis					1557:1567	24-h metabolic homeostasis	1542:1567	24-h metabolic homeostasis	1542:1567	In combination, these findings establish a key role for microbiome-dependent circadian GLP-1 secretion in the maintenance of 24-h metabolic homeostasis.
32928871	7	27	theme	microbiome	1122:1131	arg1	Analysis					1047:1054	Analysis	1047:1054	Analysis of the primary L-cell transcriptome, as well as of the intestinal microbiome,	1047:1132	Analysis of the primary L-cell transcriptome, as well as of the intestinal microbiome, also demonstrated time-of-day- and diet-dependent changes paralleling GLP-1 secretion.
32928871	5	28	theme	other	812:816	arg1	polypeptide					861:871	glucose-dependent insulinotropic polypeptide	828:871	glucose-dependent insulinotropic polypeptide	828:871	In mice fed a Western (high-fat/high-sucrose) diet for 16 weeks, GLP-1 secretion was markedly increased but arrhythmic over the 24-h day, whereas levels of the other incretin, glucose-dependent insulinotropic polypeptide, were not as profoundly affected.
32928871	5	28	theme	other	812:816	arg1	incretin					818:825	the other incretin	808:825	the other incretin	808:825	In mice fed a Western (high-fat/high-sucrose) diet for 16 weeks, GLP-1 secretion was markedly increased but arrhythmic over the 24-h day, whereas levels of the other incretin, glucose-dependent insulinotropic polypeptide, were not as profoundly affected.
32928871	0	29	theme	Circadian	0:8	arg1	Secretion					16:24	Circadian GLP-1 Secretion	0:24	Circadian GLP-1 Secretion in Mice	0:32	Circadian GLP-1 Secretion in Mice Is Dependent on the Intestinal Microbiome for Maintenance of Diurnal Metabolic Homeostasis.
32928871	8	30	from	role	1368:1371	arg1	release					1408:1414	diurnal GLP-1 release	1394:1414	diurnal GLP-1 release	1394:1414	Finally, studies in antibiotic-induced microbial depleted and in germ-free mice with and without fecal microbial transfer, provided evidence for a role of the microbiome in diurnal GLP-1 release.
32928871	9	31	theme	homeostasis	1557:1567	arg1	maintenance					1527:1537	the maintenance	1523:1537	the maintenance of 24-h metabolic homeostasis	1523:1567	In combination, these findings establish a key role for microbiome-dependent circadian GLP-1 secretion in the maintenance of 24-h metabolic homeostasis.
32928871	4	32	theme	female	639:644	arg1	mice					646:649	normal male and female mice	623:649	normal male and female mice	623:649	The circadian pattern of GLP-1 release was found to be dependent on the oral route of glucose administration and was necessary for the rhythmic release of insulin and diurnal glycemic control in normal male and female mice.
32928871	4	33	theme	administration	522:535	arg1	route					505:509	the oral route	496:509	the oral route of glucose administration	496:535	The circadian pattern of GLP-1 release was found to be dependent on the oral route of glucose administration and was necessary for the rhythmic release of insulin and diurnal glycemic control in normal male and female mice.
32928871	9	34	from	role	1464:1467	arg1	maintenance					1527:1537	the maintenance	1523:1537	the maintenance of 24-h metabolic homeostasis	1523:1567	In combination, these findings establish a key role for microbiome-dependent circadian GLP-1 secretion in the maintenance of 24-h metabolic homeostasis.
32928871	2	35	theme	highest	278:284	arg1	release					286:292	highest release	278:292	highest release at the onset of the feeding period	278:327	GLP-1 also exhibits a circadian rhythm, with highest release at the onset of the feeding period.
32928871	4	36	theme	control	612:618	arg1	release					572:578	the rhythmic release	559:578	the rhythmic release of insulin and diurnal glycemic control in normal male and female mice	559:649	The circadian pattern of GLP-1 release was found to be dependent on the oral route of glucose administration and was necessary for the rhythmic release of insulin and diurnal glycemic control in normal male and female mice.
32928871	3	37	theme	microbial	341:349	arg1	composition					351:361	microbial composition	341:361	microbial composition	341:361	Similarly, microbial composition and function exhibit circadian rhythmicity with fasting-feeding.
32928871	0	38	theme	Homeostasis	113:123	arg1	Maintenance					80:90	Maintenance	80:90	Maintenance of Diurnal Metabolic Homeostasis	80:123	Circadian GLP-1 Secretion in Mice Is Dependent on the Intestinal Microbiome for Maintenance of Diurnal Metabolic Homeostasis.
32928871	4	39	theme	glycemic	603:610	arg1	control					612:618	diurnal glycemic control	595:618	diurnal glycemic control	595:618	The circadian pattern of GLP-1 release was found to be dependent on the oral route of glucose administration and was necessary for the rhythmic release of insulin and diurnal glycemic control in normal male and female mice.
32928871	3	40	theme	circadian	384:392	arg1	rhythmicity					394:404	circadian rhythmicity	384:404	circadian rhythmicity	384:404	Similarly, microbial composition and function exhibit circadian rhythmicity with fasting-feeding.
32928871	4	41	theme	normal	623:628	arg1	mice					646:649	normal male and female mice	623:649	normal male and female mice	623:649	The circadian pattern of GLP-1 release was found to be dependent on the oral route of glucose administration and was necessary for the rhythmic release of insulin and diurnal glycemic control in normal male and female mice.
32928871	5	42	theme	incretin	818:825	arg1	levels					798:803	levels	798:803	levels of the other incretin, glucose-dependent insulinotropic polypeptide,	798:872	In mice fed a Western (high-fat/high-sucrose) diet for 16 weeks, GLP-1 secretion was markedly increased but arrhythmic over the 24-h day, whereas levels of the other incretin, glucose-dependent insulinotropic polypeptide, were not as profoundly affected.
32928871	1	43	theme	intestinal	190:199	arg1	cell					203:206	the intestinal L cell	186:206	the intestinal L cell	186:206	The incretin glucagon-like peptide 1 (GLP-1) is secreted by the intestinal L cell upon nutrient ingestion.
32928871	7	44	theme	diet-dependent	1169:1182	arg1	changes					1184:1190	time-of-day- and diet-dependent changes	1152:1190	time-of-day- and diet-dependent changes paralleling GLP-1 secretion	1152:1218	Analysis of the primary L-cell transcriptome, as well as of the intestinal microbiome, also demonstrated time-of-day- and diet-dependent changes paralleling GLP-1 secretion.
32928871	5	45	theme	high-fat/high-sucrose	675:695	arg1	diet					698:701	a Western (high-fat/high-sucrose) diet	664:701	a Western (high-fat/high-sucrose) diet for 16 weeks	664:714	In mice fed a Western (high-fat/high-sucrose) diet for 16 weeks, GLP-1 secretion was markedly increased but arrhythmic over the 24-h day, whereas levels of the other incretin, glucose-dependent insulinotropic polypeptide, were not as profoundly affected.
32928871	4	46	theme	GLP-1	453:457	arg1	release					459:465	GLP-1 release	453:465	GLP-1 release	453:465	The circadian pattern of GLP-1 release was found to be dependent on the oral route of glucose administration and was necessary for the rhythmic release of insulin and diurnal glycemic control in normal male and female mice.
32928871	4	47	theme	diurnal	595:601	arg1	control					612:618	diurnal glycemic control	595:618	diurnal glycemic control	595:618	The circadian pattern of GLP-1 release was found to be dependent on the oral route of glucose administration and was necessary for the rhythmic release of insulin and diurnal glycemic control in normal male and female mice.
32928871	1	48	theme	L	201:201	arg1	cell					203:206	the intestinal L cell	186:206	the intestinal L cell	186:206	The incretin glucagon-like peptide 1 (GLP-1) is secreted by the intestinal L cell upon nutrient ingestion.
32928871	4	49	theme	oral	500:503	arg1	route					505:509	the oral route	496:509	the oral route of glucose administration	496:535	The circadian pattern of GLP-1 release was found to be dependent on the oral route of glucose administration and was necessary for the rhythmic release of insulin and diurnal glycemic control in normal male and female mice.
32928871	8	50	theme	microbiome	1380:1389	arg1	role					1368:1371	a role	1366:1371	a role of the microbiome in diurnal GLP-1 release	1366:1414	Finally, studies in antibiotic-induced microbial depleted and in germ-free mice with and without fecal microbial transfer, provided evidence for a role of the microbiome in diurnal GLP-1 release.
32928871	9	51	theme	key	1460:1462	arg1	role					1464:1467	a key role	1458:1467	a key role for microbiome-dependent circadian GLP-1 secretion in the maintenance of 24-h metabolic homeostasis	1458:1567	In combination, these findings establish a key role for microbiome-dependent circadian GLP-1 secretion in the maintenance of 24-h metabolic homeostasis.
32928871	6	52	from	changes	924:930	arg1	secretion					941:949	GLP-1 secretion	935:949	GLP-1 secretion	935:949	Furthermore, the changes in GLP-1 secretion were shown to be essential for the maintenance of normoglycemia in this obesogenic environment.
32928871	4	53	theme	glucose	514:520	arg1	administration					522:535	glucose administration	514:535	glucose administration	514:535	The circadian pattern of GLP-1 release was found to be dependent on the oral route of glucose administration and was necessary for the rhythmic release of insulin and diurnal glycemic control in normal male and female mice.
32928871	9	54	theme	circadian	1494:1502	arg1	secretion					1510:1518	microbiome-dependent circadian GLP-1 secretion	1473:1518	microbiome-dependent circadian GLP-1 secretion	1473:1518	In combination, these findings establish a key role for microbiome-dependent circadian GLP-1 secretion in the maintenance of 24-h metabolic homeostasis.
32928871	8	55	theme	GLP-1	1402:1406	arg1	release					1408:1414	diurnal GLP-1 release	1394:1414	diurnal GLP-1 release	1394:1414	Finally, studies in antibiotic-induced microbial depleted and in germ-free mice with and without fecal microbial transfer, provided evidence for a role of the microbiome in diurnal GLP-1 release.
32928871	8	56	theme	diurnal	1394:1400	arg1	release					1408:1414	diurnal GLP-1 release	1394:1414	diurnal GLP-1 release	1394:1414	Finally, studies in antibiotic-induced microbial depleted and in germ-free mice with and without fecal microbial transfer, provided evidence for a role of the microbiome in diurnal GLP-1 release.
32928871	4	57	theme	circadian	432:440	arg1	pattern					442:448	The circadian pattern	428:448	The circadian pattern of GLP-1 release	428:465	The circadian pattern of GLP-1 release was found to be dependent on the oral route of glucose administration and was necessary for the rhythmic release of insulin and diurnal glycemic control in normal male and female mice.
32928871	4	57	theme	circadian	432:440	arg1	dependent					483:491	dependent	483:491	dependent	483:491	The circadian pattern of GLP-1 release was found to be dependent on the oral route of glucose administration and was necessary for the rhythmic release of insulin and diurnal glycemic control in normal male and female mice.
32928871	4	57	theme	circadian	432:440	arg1	necessary					545:553	necessary	545:553	necessary	545:553	The circadian pattern of GLP-1 release was found to be dependent on the oral route of glucose administration and was necessary for the rhythmic release of insulin and diurnal glycemic control in normal male and female mice.
32928871	8	58	theme	germ-free	1286:1294	arg1	mice					1296:1299	germ-free mice	1286:1299	germ-free mice with and without fecal microbial transfer	1286:1341	Finally, studies in antibiotic-induced microbial depleted and in germ-free mice with and without fecal microbial transfer, provided evidence for a role of the microbiome in diurnal GLP-1 release.
32928871	4	59	theme	insulin	583:589	arg1	release					572:578	the rhythmic release	559:578	the rhythmic release of insulin and diurnal glycemic control in normal male and female mice	559:649	The circadian pattern of GLP-1 release was found to be dependent on the oral route of glucose administration and was necessary for the rhythmic release of insulin and diurnal glycemic control in normal male and female mice.
32928871	9	60	theme	24-h	1542:1545	arg1	homeostasis					1557:1567	24-h metabolic homeostasis	1542:1567	24-h metabolic homeostasis	1542:1567	In combination, these findings establish a key role for microbiome-dependent circadian GLP-1 secretion in the maintenance of 24-h metabolic homeostasis.
32928871	8	61	theme	microbial	1324:1332	arg1	transfer					1334:1341	fecal microbial transfer	1318:1341	fecal microbial transfer	1318:1341	Finally, studies in antibiotic-induced microbial depleted and in germ-free mice with and without fecal microbial transfer, provided evidence for a role of the microbiome in diurnal GLP-1 release.
32928871	1	62	theme	incretin	130:137	arg1	GLP-1					164:168	The incretin glucagon-like peptide 1 (GLP-1)	126:169	The incretin glucagon-like peptide 1 (GLP-1)	126:169	The incretin glucagon-like peptide 1 (GLP-1) is secreted by the intestinal L cell upon nutrient ingestion.
32928871	8	63	theme	fecal	1318:1322	arg1	transfer					1334:1341	fecal microbial transfer	1318:1341	fecal microbial transfer	1318:1341	Finally, studies in antibiotic-induced microbial depleted and in germ-free mice with and without fecal microbial transfer, provided evidence for a role of the microbiome in diurnal GLP-1 release.
32928871	0	64	theme	Intestinal	54:63	arg1	Microbiome					65:74	the Intestinal Microbiome	50:74	the Intestinal Microbiome for Maintenance of Diurnal Metabolic Homeostasis	50:123	Circadian GLP-1 Secretion in Mice Is Dependent on the Intestinal Microbiome for Maintenance of Diurnal Metabolic Homeostasis.
32928871	6	65	theme	normoglycemia	1001:1013	arg1	maintenance					986:996	the maintenance	982:996	the maintenance of normoglycemia in this obesogenic environment	982:1044	Furthermore, the changes in GLP-1 secretion were shown to be essential for the maintenance of normoglycemia in this obesogenic environment.
32928871	1	66	theme	glucagon-like	139:151	arg1	GLP-1					164:168	The incretin glucagon-like peptide 1 (GLP-1)	126:169	The incretin glucagon-like peptide 1 (GLP-1)	126:169	The incretin glucagon-like peptide 1 (GLP-1) is secreted by the intestinal L cell upon nutrient ingestion.
32928871	6	67	theme	obesogenic	1023:1032	arg1	environment					1034:1044	this obesogenic environment	1018:1044	this obesogenic environment	1018:1044	Furthermore, the changes in GLP-1 secretion were shown to be essential for the maintenance of normoglycemia in this obesogenic environment.
32928871	1	68	theme	nutrient	213:220	arg1	ingestion					222:230	nutrient ingestion	213:230	nutrient ingestion	213:230	The incretin glucagon-like peptide 1 (GLP-1) is secreted by the intestinal L cell upon nutrient ingestion.
32928871	7	69	theme	GLP-1	1204:1208	arg1	secretion					1210:1218	GLP-1 secretion	1204:1218	GLP-1 secretion	1204:1218	Analysis of the primary L-cell transcriptome, as well as of the intestinal microbiome, also demonstrated time-of-day- and diet-dependent changes paralleling GLP-1 secretion.
32928871	5	70	theme	24-h	780:783	arg1	day					785:787	the 24-h day	776:787	the 24-h day	776:787	In mice fed a Western (high-fat/high-sucrose) diet for 16 weeks, GLP-1 secretion was markedly increased but arrhythmic over the 24-h day, whereas levels of the other incretin, glucose-dependent insulinotropic polypeptide, were not as profoundly affected.
32928871	1	71	theme	peptide	153:159	arg1	GLP-1					164:168	The incretin glucagon-like peptide 1 (GLP-1)	126:169	The incretin glucagon-like peptide 1 (GLP-1)	126:169	The incretin glucagon-like peptide 1 (GLP-1) is secreted by the intestinal L cell upon nutrient ingestion.
32897850	3	0	theme	polyphasic	271:280	arg1	approach					282:289	a polyphasic approach	269:289	a polyphasic approach	269:289	The taxonomic position of the strain was characterized using a polyphasic approach.
32897850	10	1	theme	Nocardia	950:957	arg1	11307T					972:977	Nocardia veterana JCM 11307T	950:977	Nocardia veterana JCM 11307T (98.4 %)	950:986	Strain CT2-14T showed the highest 16S rRNA gene similarity to Nocardia veterana JCM 11307T (98.4 %), Nocardia africana JCM 11438T (98.2 %) and Nocardia kruczakiae JCM 13032T (98.0 %).
32897850	10	1	theme	Nocardia	950:957	arg1	%					985:985	98.4 %	980:985	98.4 %	980:985	Strain CT2-14T showed the highest 16S rRNA gene similarity to Nocardia veterana JCM 11307T (98.4 %), Nocardia africana JCM 11438T (98.2 %) and Nocardia kruczakiae JCM 13032T (98.0 %).
32897850	11	2	theme	CT2-14T	1099:1105	arg1	7.37 Mb					1111:1117	7.37 Mb	1111:1117	7.37 Mb	1111:1117	The draft genome of strain CT2-14T was 7.37 Mb with 6685 coding sequences with an average G+C content of 67.9 mol %.
32897850	11	2	theme	CT2-14T	1099:1105	arg1	genome					1082:1087	The draft genome	1072:1087	The draft genome of strain CT2-14T	1072:1105	The draft genome of strain CT2-14T was 7.37 Mb with 6685 coding sequences with an average G+C content of 67.9 mol %.
32897850	10	3	theme	JCM	968:970	arg1	11307T					972:977	Nocardia veterana JCM 11307T	950:977	Nocardia veterana JCM 11307T (98.4 %)	950:986	Strain CT2-14T showed the highest 16S rRNA gene similarity to Nocardia veterana JCM 11307T (98.4 %), Nocardia africana JCM 11438T (98.2 %) and Nocardia kruczakiae JCM 13032T (98.0 %).
32897850	10	3	theme	JCM	968:970	arg1	%					985:985	98.4 %	980:985	98.4 %	980:985	Strain CT2-14T showed the highest 16S rRNA gene similarity to Nocardia veterana JCM 11307T (98.4 %), Nocardia africana JCM 11438T (98.2 %) and Nocardia kruczakiae JCM 13032T (98.0 %).
32897850	11	4	theme	draft	1076:1080	arg1	7.37 Mb					1111:1117	7.37 Mb	1111:1117	7.37 Mb	1111:1117	The draft genome of strain CT2-14T was 7.37 Mb with 6685 coding sequences with an average G+C content of 67.9 mol %.
32897850	11	4	theme	draft	1076:1080	arg1	genome					1082:1087	The draft genome	1072:1087	The draft genome of strain CT2-14T	1072:1105	The draft genome of strain CT2-14T was 7.37 Mb with 6685 coding sequences with an average G+C content of 67.9 mol %.
32897850	4	5	theme	%	421:421	arg1	NaCl					429:432	4 % (w/v) NaCl	419:432	4 % (w/v) NaCl agar plate	419:443	The strain grew at 15-40 °C (optimum, 28-37 °C), pH 6-11 (optimum, pH 6-8) and on an International Streptomyces Project 2 with 4 % (w/v) NaCl agar plate.
32897850	10	6	theme	16S	922:924	arg1	similarity					936:945	the highest 16S rRNA gene similarity	910:945	the highest 16S rRNA gene similarity to Nocardia veterana JCM 11307T (98.4 %), Nocardia africana JCM 11438T (98.2 %) and Nocardia kruczakiae JCM 13032T (98.0 %)	910:1069	Strain CT2-14T showed the highest 16S rRNA gene similarity to Nocardia veterana JCM 11307T (98.4 %), Nocardia africana JCM 11438T (98.2 %) and Nocardia kruczakiae JCM 13032T (98.0 %).
32897850	10	7	theme	Strain	888:893	arg1	CT2-14T					895:901	Strain CT2-14T	888:901	Strain CT2-14T	888:901	Strain CT2-14T showed the highest 16S rRNA gene similarity to Nocardia veterana JCM 11307T (98.4 %), Nocardia africana JCM 11438T (98.2 %) and Nocardia kruczakiae JCM 13032T (98.0 %).
32897850	1	8	from	soil	44:47	arg1	Thailand					52:59	Thailand	52:59	Thailand	52:59	nov., isolated from soil in Thailand.
32897850	10	9	theme	JCM	1051:1053	arg1	%					1068:1068	98.0 %	1063:1068	98.0 %	1063:1068	Strain CT2-14T showed the highest 16S rRNA gene similarity to Nocardia veterana JCM 11307T (98.4 %), Nocardia africana JCM 11438T (98.2 %) and Nocardia kruczakiae JCM 13032T (98.0 %).
32897850	10	9	theme	JCM	1051:1053	arg1	13032T					1055:1060	Nocardia kruczakiae JCM 13032T	1031:1060	Nocardia kruczakiae JCM 13032T (98.0 %)	1031:1069	Strain CT2-14T showed the highest 16S rRNA gene similarity to Nocardia veterana JCM 11307T (98.4 %), Nocardia africana JCM 11438T (98.2 %) and Nocardia kruczakiae JCM 13032T (98.0 %).
32897850	5	10	theme	cell-wall	491:499	arg1	peptidoglycan					501:513	the cell-wall peptidoglycan	487:513	the cell-wall peptidoglycan	487:513	Meso-diaminopimelic acid was detected in the cell-wall peptidoglycan.
32897850	12	11	theme	phylogenomic	1202:1213	arg1	analysis					1220:1227	the phylogenomic tree analysis	1198:1227	the phylogenomic tree analysis	1198:1227	Based on the phylogenomic tree analysis, the strain was closely related to Nocardia niigatensis NBRC 100131T.
32897850	15	12	theme	=JCM	1501:1504	arg1	2838T					1519:1523	=JCM 33775T=TISTR 2838T	1501:1523	=JCM 33775T=TISTR 2838T	1501:1523	The type strain is CT2-14T (=JCM 33775T=TISTR 2838T).
32897850	15	12	theme	=JCM	1501:1504	arg1	CT2-14T					1492:1498	CT2-14T	1492:1498	CT2-14T (=JCM 33775T=TISTR 2838T)	1492:1524	The type strain is CT2-14T (=JCM 33775T=TISTR 2838T).
32897850	10	13	theme	kruczakiae	1040:1049	arg1	%					1068:1068	98.0 %	1063:1068	98.0 %	1063:1068	Strain CT2-14T showed the highest 16S rRNA gene similarity to Nocardia veterana JCM 11307T (98.4 %), Nocardia africana JCM 11438T (98.2 %) and Nocardia kruczakiae JCM 13032T (98.0 %).
32897850	10	13	theme	kruczakiae	1040:1049	arg1	13032T					1055:1060	Nocardia kruczakiae JCM 13032T	1031:1060	Nocardia kruczakiae JCM 13032T (98.0 %)	1031:1069	Strain CT2-14T showed the highest 16S rRNA gene similarity to Nocardia veterana JCM 11307T (98.4 %), Nocardia africana JCM 11438T (98.2 %) and Nocardia kruczakiae JCM 13032T (98.0 %).
32897850	9	14	theme	major	778:782	arg1	phosphatidylethanolamine					802:825	phosphatidylethanolamine	802:825	phosphatidylethanolamine	802:825	The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylinositol mannosides.
32897850	9	14	theme	major	778:782	arg1	lipids					790:795	The major polar lipids	774:795	The major polar lipids	774:795	The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylinositol mannosides.
32897850	4	15	theme	International	377:389	arg1	Project					404:410	an International Streptomyces Project 2	374:412	an International Streptomyces Project 2 with 4 % (w/v) NaCl agar plate	374:443	The strain grew at 15-40 °C (optimum, 28-37 °C), pH 6-11 (optimum, pH 6-8) and on an International Streptomyces Project 2 with 4 % (w/v) NaCl agar plate.
32897850	11	16	theme	strain	1092:1097	arg1	CT2-14T					1099:1105	strain CT2-14T	1092:1105	strain CT2-14T	1092:1105	The draft genome of strain CT2-14T was 7.37 Mb with 6685 coding sequences with an average G+C content of 67.9 mol %.
32897850	15	17	theme	type	1477:1480	arg1	strain					1482:1487	The type strain	1473:1487	The type strain	1473:1487	The type strain is CT2-14T (=JCM 33775T=TISTR 2838T).
32897850	15	17	theme	type	1477:1480	arg1	CT2-14T					1492:1498	CT2-14T	1492:1498	CT2-14T (=JCM 33775T=TISTR 2838T)	1492:1524	The type strain is CT2-14T (=JCM 33775T=TISTR 2838T).
32897850	13	18	theme	genus	1397:1401	arg1	Nocardia					1403:1410	the genus Nocardia	1393:1410	the genus Nocardia	1393:1410	On the basis of polyphasic and genome analyses, strain CT2-14T represented a novel species of the genus Nocardia for which the name Nocardia aurantiaca sp.
32897850	8	19	theme	 0	675:676	arg1	10-methyl					678:686	 0 10-methyl	675:686	 0 10-methyl	675:686	The strain contained C16 : 0, summed feature 3, C17 : 0 10-methyl and C18 : 1 ω9c as the major fatty acids and MK-8(H4ω-cycl) as the major menaquinone.
32897850	9	20	theme	polar	784:788	arg1	phosphatidylethanolamine					802:825	phosphatidylethanolamine	802:825	phosphatidylethanolamine	802:825	The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylinositol mannosides.
32897850	9	20	theme	polar	784:788	arg1	lipids					790:795	The major polar lipids	774:795	The major polar lipids	774:795	The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylinositol mannosides.
32897850	2	21	theme	genus	117:121	arg1	Nocardia					123:130	the genus Nocardia	113:130	the genus Nocardia	113:130	A novel actinomycete strain, CT2-14T, belonging to the genus Nocardia, was isolated from a soil sample collected from Phichit Province, Thailand.
32897850	13	22	theme	Nocardia	1403:1410	arg1	species					1382:1388	a novel species	1374:1388	a novel species of the genus Nocardia for which the name Nocardia aurantiaca sp	1374:1452	On the basis of polyphasic and genome analyses, strain CT2-14T represented a novel species of the genus Nocardia for which the name Nocardia aurantiaca sp.
32897850	8	23	theme	 1	697:698	arg1	ω9c					700:702	 1 ω9c	697:702	C16 : 0, summed feature 3, C17 : 0 10-methyl and C18 : 1 ω9c	643:702	The strain contained C16 : 0, summed feature 3, C17 : 0 10-methyl and C18 : 1 ω9c as the major fatty acids and MK-8(H4ω-cycl) as the major menaquinone.
32897850	13	24	theme	Nocardia	1431:1438	arg1	sp					1451:1452	the name Nocardia aurantiaca sp	1422:1452	the name Nocardia aurantiaca sp	1422:1452	On the basis of polyphasic and genome analyses, strain CT2-14T represented a novel species of the genus Nocardia for which the name Nocardia aurantiaca sp.
32897850	10	25	theme	Nocardia	1031:1038	arg1	%					1068:1068	98.0 %	1063:1068	98.0 %	1063:1068	Strain CT2-14T showed the highest 16S rRNA gene similarity to Nocardia veterana JCM 11307T (98.4 %), Nocardia africana JCM 11438T (98.2 %) and Nocardia kruczakiae JCM 13032T (98.0 %).
32897850	10	25	theme	Nocardia	1031:1038	arg1	13032T					1055:1060	Nocardia kruczakiae JCM 13032T	1031:1060	Nocardia kruczakiae JCM 13032T (98.0 %)	1031:1069	Strain CT2-14T showed the highest 16S rRNA gene similarity to Nocardia veterana JCM 11307T (98.4 %), Nocardia africana JCM 11438T (98.2 %) and Nocardia kruczakiae JCM 13032T (98.0 %).
32897850	3	26	theme	taxonomic	212:220	arg1	position					222:229	The taxonomic position	208:229	The taxonomic position of the strain	208:243	The taxonomic position of the strain was characterized using a polyphasic approach.
32897850	11	27	theme	average	1154:1160	arg1	content					1166:1172	an average G+C content	1151:1172	an average G+C content of 67.9 mol %	1151:1186	The draft genome of strain CT2-14T was 7.37 Mb with 6685 coding sequences with an average G+C content of 67.9 mol %.
32897850	2	28	theme	novel	64:68	arg1	CT2-14T					91:97	CT2-14T	91:97	CT2-14T	91:97	A novel actinomycete strain, CT2-14T, belonging to the genus Nocardia, was isolated from a soil sample collected from Phichit Province, Thailand.
32897850	2	28	theme	novel	64:68	arg1	strain					83:88	A novel actinomycete strain	62:88	A novel actinomycete strain	62:88	A novel actinomycete strain, CT2-14T, belonging to the genus Nocardia, was isolated from a soil sample collected from Phichit Province, Thailand.
32897850	0	29	theme	aurantiaca	9:18	arg1	sp					20:21	Nocardia aurantiaca sp	0:21	Nocardia aurantiaca sp.	0:22	Nocardia aurantiaca sp.
32897850	4	30	theme	Streptomyces	391:402	arg1	Project					404:410	an International Streptomyces Project 2	374:412	an International Streptomyces Project 2 with 4 % (w/v) NaCl agar plate	374:443	The strain grew at 15-40 °C (optimum, 28-37 °C), pH 6-11 (optimum, pH 6-8) and on an International Streptomyces Project 2 with 4 % (w/v) NaCl agar plate.
32897850	11	31	with	sequences	1136:1144	arg1	content					1166:1172	an average G+C content	1151:1172	an average G+C content of 67.9 mol %	1151:1186	The draft genome of strain CT2-14T was 7.37 Mb with 6685 coding sequences with an average G+C content of 67.9 mol %.
32897850	0	32	theme	Nocardia	0:7	arg1	sp					20:21	Nocardia aurantiaca sp	0:21	Nocardia aurantiaca sp.	0:22	Nocardia aurantiaca sp.
32897850	10	33	theme	Nocardia	989:996	arg1	11438T					1011:1016	Nocardia africana JCM 11438T	989:1016	Nocardia africana JCM 11438T (98.2 %)	989:1025	Strain CT2-14T showed the highest 16S rRNA gene similarity to Nocardia veterana JCM 11307T (98.4 %), Nocardia africana JCM 11438T (98.2 %) and Nocardia kruczakiae JCM 13032T (98.0 %).
32897850	10	33	theme	Nocardia	989:996	arg1	%					1024:1024	98.2 %	1019:1024	98.2 %	1019:1024	Strain CT2-14T showed the highest 16S rRNA gene similarity to Nocardia veterana JCM 11307T (98.4 %), Nocardia africana JCM 11438T (98.2 %) and Nocardia kruczakiae JCM 13032T (98.0 %).
32897850	12	34	theme	NBRC	1285:1288	arg1	100131T					1290:1296	Nocardia niigatensis NBRC 100131T	1264:1296	Nocardia niigatensis NBRC 100131T	1264:1296	Based on the phylogenomic tree analysis, the strain was closely related to Nocardia niigatensis NBRC 100131T.
32897850	4	35	theme	agar	434:437	arg1	plate					439:443	4 % (w/v) NaCl agar plate	419:443	4 % (w/v) NaCl agar plate	419:443	The strain grew at 15-40 °C (optimum, 28-37 °C), pH 6-11 (optimum, pH 6-8) and on an International Streptomyces Project 2 with 4 % (w/v) NaCl agar plate.
32897850	13	36	theme	aurantiaca	1440:1449	arg1	sp					1451:1452	the name Nocardia aurantiaca sp	1422:1452	the name Nocardia aurantiaca sp	1422:1452	On the basis of polyphasic and genome analyses, strain CT2-14T represented a novel species of the genus Nocardia for which the name Nocardia aurantiaca sp.
32897850	11	37	theme	%	1186:1186	arg1	content					1166:1172	an average G+C content	1151:1172	an average G+C content of 67.9 mol %	1151:1186	The draft genome of strain CT2-14T was 7.37 Mb with 6685 coding sequences with an average G+C content of 67.9 mol %.
32897850	2	38	attach	isolated	137:144	arg2	strain					83:88	A novel actinomycete strain	62:88	A novel actinomycete strain	62:88	A novel actinomycete strain, CT2-14T, belonging to the genus Nocardia, was isolated from a soil sample collected from Phichit Province, Thailand.
32897850	2	38	attach	isolated	137:144	arg1	sample					158:163	a soil sample	151:163	a soil sample collected from Phichit Province, Thailand	151:205	A novel actinomycete strain, CT2-14T, belonging to the genus Nocardia, was isolated from a soil sample collected from Phichit Province, Thailand.
32897850	2	38	attach	isolated	137:144	arg2	CT2-14T					91:97	CT2-14T	91:97	CT2-14T	91:97	A novel actinomycete strain, CT2-14T, belonging to the genus Nocardia, was isolated from a soil sample collected from Phichit Province, Thailand.
32897850	13	39	theme	novel	1376:1380	arg1	species					1382:1388	a novel species	1374:1388	a novel species of the genus Nocardia for which the name Nocardia aurantiaca sp	1374:1452	On the basis of polyphasic and genome analyses, strain CT2-14T represented a novel species of the genus Nocardia for which the name Nocardia aurantiaca sp.
32897850	3	40	theme	strain	238:243	arg1	position					222:229	The taxonomic position	208:229	The taxonomic position of the strain	208:243	The taxonomic position of the strain was characterized using a polyphasic approach.
32897850	6	41	theme	whole-cell	569:578	arg1	hydrolysates					580:591	its whole-cell hydrolysates	565:591	its whole-cell hydrolysates	565:591	Ribose, arabinose and galactose were detected in its whole-cell hydrolysates.
32897850	13	42	dep	analyses	1337:1344	arg1	the					1302:1304	the	1302:1304	the	1302:1304	On the basis of polyphasic and genome analyses, strain CT2-14T represented a novel species of the genus Nocardia for which the name Nocardia aurantiaca sp.
32897850	13	42	dep	analyses	1337:1344	arg1	basis					1306:1310	basis	1306:1310	basis	1306:1310	On the basis of polyphasic and genome analyses, strain CT2-14T represented a novel species of the genus Nocardia for which the name Nocardia aurantiaca sp.
32897850	8	43	theme	fatty	717:721	arg1	acids					723:727	the major fatty acids	707:727	the major fatty acids	707:727	The strain contained C16 : 0, summed feature 3, C17 : 0 10-methyl and C18 : 1 ω9c as the major fatty acids and MK-8(H4ω-cycl) as the major menaquinone.
32897850	8	43	theme	fatty	717:721	arg1	C16 					643:646	C16 	643:646	C16 : 0, summed feature 3, C17 : 0 10-methyl and C18 : 1 ω9c	643:702	The strain contained C16 : 0, summed feature 3, C17 : 0 10-methyl and C18 : 1 ω9c as the major fatty acids and MK-8(H4ω-cycl) as the major menaquinone.
32897850	8	44	theme	major	711:715	arg1	acids					723:727	the major fatty acids	707:727	the major fatty acids	707:727	The strain contained C16 : 0, summed feature 3, C17 : 0 10-methyl and C18 : 1 ω9c as the major fatty acids and MK-8(H4ω-cycl) as the major menaquinone.
32897850	8	44	theme	major	711:715	arg1	C16 					643:646	C16 	643:646	C16 : 0, summed feature 3, C17 : 0 10-methyl and C18 : 1 ω9c	643:702	The strain contained C16 : 0, summed feature 3, C17 : 0 10-methyl and C18 : 1 ω9c as the major fatty acids and MK-8(H4ω-cycl) as the major menaquinone.
32897850	12	45	theme	niigatensis	1273:1283	arg1	100131T					1290:1296	Nocardia niigatensis NBRC 100131T	1264:1296	Nocardia niigatensis NBRC 100131T	1264:1296	Based on the phylogenomic tree analysis, the strain was closely related to Nocardia niigatensis NBRC 100131T.
32897850	9	46	theme	phosphatidylinositol	855:874	arg1	mannosides					876:885	phosphatidylinositol mannosides	855:885	phosphatidylinositol mannosides	855:885	The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylinositol mannosides.
32897850	4	47	theme	w/v	424:426	arg1	NaCl					429:432	4 % (w/v) NaCl	419:432	4 % (w/v) NaCl agar plate	419:443	The strain grew at 15-40 °C (optimum, 28-37 °C), pH 6-11 (optimum, pH 6-8) and on an International Streptomyces Project 2 with 4 % (w/v) NaCl agar plate.
32897850	7	48	theme	Mycolic	594:600	arg1	acids					602:606	Mycolic acids	594:606	Mycolic acids	594:606	Mycolic acids were present.
32897850	13	49	theme	strain	1347:1352	arg1	CT2-14T					1354:1360	strain CT2-14T	1347:1360	strain CT2-14T	1347:1360	On the basis of polyphasic and genome analyses, strain CT2-14T represented a novel species of the genus Nocardia for which the name Nocardia aurantiaca sp.
32897850	8	50	theme	major	755:759	arg1	menaquinone					761:771	the major menaquinone	751:771	the major menaquinone	751:771	The strain contained C16 : 0, summed feature 3, C17 : 0 10-methyl and C18 : 1 ω9c as the major fatty acids and MK-8(H4ω-cycl) as the major menaquinone.
32897850	8	50	theme	major	755:759	arg1	C16 					643:646	C16 	643:646	C16 : 0, summed feature 3, C17 : 0 10-methyl and C18 : 1 ω9c	643:702	The strain contained C16 : 0, summed feature 3, C17 : 0 10-methyl and C18 : 1 ω9c as the major fatty acids and MK-8(H4ω-cycl) as the major menaquinone.
32897850	10	51	theme	JCM	1007:1009	arg1	11438T					1011:1016	Nocardia africana JCM 11438T	989:1016	Nocardia africana JCM 11438T (98.2 %)	989:1025	Strain CT2-14T showed the highest 16S rRNA gene similarity to Nocardia veterana JCM 11307T (98.4 %), Nocardia africana JCM 11438T (98.2 %) and Nocardia kruczakiae JCM 13032T (98.0 %).
32897850	10	51	theme	JCM	1007:1009	arg1	%					1024:1024	98.2 %	1019:1024	98.2 %	1019:1024	Strain CT2-14T showed the highest 16S rRNA gene similarity to Nocardia veterana JCM 11307T (98.4 %), Nocardia africana JCM 11438T (98.2 %) and Nocardia kruczakiae JCM 13032T (98.0 %).
32897850	2	52	theme	actinomycete	70:81	arg1	CT2-14T					91:97	CT2-14T	91:97	CT2-14T	91:97	A novel actinomycete strain, CT2-14T, belonging to the genus Nocardia, was isolated from a soil sample collected from Phichit Province, Thailand.
32897850	2	52	theme	actinomycete	70:81	arg1	strain					83:88	A novel actinomycete strain	62:88	A novel actinomycete strain	62:88	A novel actinomycete strain, CT2-14T, belonging to the genus Nocardia, was isolated from a soil sample collected from Phichit Province, Thailand.
32897850	6	53	located	detected	553:560	arg1	hydrolysates					580:591	its whole-cell hydrolysates	565:591	its whole-cell hydrolysates	565:591	Ribose, arabinose and galactose were detected in its whole-cell hydrolysates.
32897850	6	53	located	detected	553:560	arg2	Ribose					516:521	Ribose	516:521	Ribose	516:521	Ribose, arabinose and galactose were detected in its whole-cell hydrolysates.
32897850	6	53	located	detected	553:560	arg2	galactose					538:546	galactose	538:546	galactose	538:546	Ribose, arabinose and galactose were detected in its whole-cell hydrolysates.
32897850	6	53	located	detected	553:560	arg2	arabinose					524:532	arabinose	524:532	arabinose	524:532	Ribose, arabinose and galactose were detected in its whole-cell hydrolysates.
32897850	8	54	dep	C16 	643:646	arg1	C18 					692:695	C18 	692:695	C18 	692:695	The strain contained C16 : 0, summed feature 3, C17 : 0 10-methyl and C18 : 1 ω9c as the major fatty acids and MK-8(H4ω-cycl) as the major menaquinone.
32897850	8	54	dep	C16 	643:646	arg1	 0					648:649	 0	648:649	 0	648:649	The strain contained C16 : 0, summed feature 3, C17 : 0 10-methyl and C18 : 1 ω9c as the major fatty acids and MK-8(H4ω-cycl) as the major menaquinone.
32897850	8	54	dep	C16 	643:646	arg1	C17 					670:673	C17 	670:673	C17 	670:673	The strain contained C16 : 0, summed feature 3, C17 : 0 10-methyl and C18 : 1 ω9c as the major fatty acids and MK-8(H4ω-cycl) as the major menaquinone.
32897850	8	54	dep	C16 	643:646	arg1	ω9c					700:702	 1 ω9c	697:702	C16 : 0, summed feature 3, C17 : 0 10-methyl and C18 : 1 ω9c	643:702	The strain contained C16 : 0, summed feature 3, C17 : 0 10-methyl and C18 : 1 ω9c as the major fatty acids and MK-8(H4ω-cycl) as the major menaquinone.
32897850	8	54	dep	C16 	643:646	arg1	10-methyl					678:686	 0 10-methyl	675:686	 0 10-methyl	675:686	The strain contained C16 : 0, summed feature 3, C17 : 0 10-methyl and C18 : 1 ω9c as the major fatty acids and MK-8(H4ω-cycl) as the major menaquinone.
32897850	8	54	dep	C16 	643:646	arg1	feature					659:665	summed feature 3	652:667	summed feature 3	652:667	The strain contained C16 : 0, summed feature 3, C17 : 0 10-methyl and C18 : 1 ω9c as the major fatty acids and MK-8(H4ω-cycl) as the major menaquinone.
32897850	11	55	with	7.37 Mb	1111:1117	arg1	sequences					1136:1144	6685 coding sequences	1124:1144	6685 coding sequences with an average G+C content of 67.9 mol %	1124:1186	The draft genome of strain CT2-14T was 7.37 Mb with 6685 coding sequences with an average G+C content of 67.9 mol %.
32897850	5	56	located	detected	475:482	arg2	acid					466:469	Meso-diaminopimelic acid	446:469	Meso-diaminopimelic acid	446:469	Meso-diaminopimelic acid was detected in the cell-wall peptidoglycan.
32897850	5	56	located	detected	475:482	arg1	peptidoglycan					501:513	the cell-wall peptidoglycan	487:513	the cell-wall peptidoglycan	487:513	Meso-diaminopimelic acid was detected in the cell-wall peptidoglycan.
32897850	4	57	theme	NaCl	429:432	arg1	plate					439:443	4 % (w/v) NaCl agar plate	419:443	4 % (w/v) NaCl agar plate	419:443	The strain grew at 15-40 °C (optimum, 28-37 °C), pH 6-11 (optimum, pH 6-8) and on an International Streptomyces Project 2 with 4 % (w/v) NaCl agar plate.
32897850	10	58	theme	gene	931:934	arg1	similarity					936:945	the highest 16S rRNA gene similarity	910:945	the highest 16S rRNA gene similarity to Nocardia veterana JCM 11307T (98.4 %), Nocardia africana JCM 11438T (98.2 %) and Nocardia kruczakiae JCM 13032T (98.0 %)	910:1069	Strain CT2-14T showed the highest 16S rRNA gene similarity to Nocardia veterana JCM 11307T (98.4 %), Nocardia africana JCM 11438T (98.2 %) and Nocardia kruczakiae JCM 13032T (98.0 %).
32897850	11	59	theme	coding	1129:1134	arg1	sequences					1136:1144	6685 coding sequences	1124:1144	6685 coding sequences with an average G+C content of 67.9 mol %	1124:1186	The draft genome of strain CT2-14T was 7.37 Mb with 6685 coding sequences with an average G+C content of 67.9 mol %.
32897850	10	60	theme	africana	998:1005	arg1	11438T					1011:1016	Nocardia africana JCM 11438T	989:1016	Nocardia africana JCM 11438T (98.2 %)	989:1025	Strain CT2-14T showed the highest 16S rRNA gene similarity to Nocardia veterana JCM 11307T (98.4 %), Nocardia africana JCM 11438T (98.2 %) and Nocardia kruczakiae JCM 13032T (98.0 %).
32897850	10	60	theme	africana	998:1005	arg1	%					1024:1024	98.2 %	1019:1024	98.2 %	1019:1024	Strain CT2-14T showed the highest 16S rRNA gene similarity to Nocardia veterana JCM 11307T (98.4 %), Nocardia africana JCM 11438T (98.2 %) and Nocardia kruczakiae JCM 13032T (98.0 %).
32897850	13	61	theme	name	1426:1429	arg1	sp					1451:1452	the name Nocardia aurantiaca sp	1422:1452	the name Nocardia aurantiaca sp	1422:1452	On the basis of polyphasic and genome analyses, strain CT2-14T represented a novel species of the genus Nocardia for which the name Nocardia aurantiaca sp.
32897850	10	62	theme	rRNA	926:929	arg1	similarity					936:945	the highest 16S rRNA gene similarity	910:945	the highest 16S rRNA gene similarity to Nocardia veterana JCM 11307T (98.4 %), Nocardia africana JCM 11438T (98.2 %) and Nocardia kruczakiae JCM 13032T (98.0 %)	910:1069	Strain CT2-14T showed the highest 16S rRNA gene similarity to Nocardia veterana JCM 11307T (98.4 %), Nocardia africana JCM 11438T (98.2 %) and Nocardia kruczakiae JCM 13032T (98.0 %).
32897850	13	63	theme	polyphasic	1315:1324	arg1	analyses					1337:1344	polyphasic and genome analyses	1315:1344	analyses	1337:1344	On the basis of polyphasic and genome analyses, strain CT2-14T represented a novel species of the genus Nocardia for which the name Nocardia aurantiaca sp.
32897850	5	64	theme	Meso-diaminopimelic	446:464	arg1	acid					466:469	Meso-diaminopimelic acid	446:469	Meso-diaminopimelic acid	446:469	Meso-diaminopimelic acid was detected in the cell-wall peptidoglycan.
32897850	12	65	theme	Nocardia	1264:1271	arg1	100131T					1290:1296	Nocardia niigatensis NBRC 100131T	1264:1296	Nocardia niigatensis NBRC 100131T	1264:1296	Based on the phylogenomic tree analysis, the strain was closely related to Nocardia niigatensis NBRC 100131T.
32897850	12	66	theme	tree	1215:1218	arg1	analysis					1220:1227	the phylogenomic tree analysis	1198:1227	the phylogenomic tree analysis	1198:1227	Based on the phylogenomic tree analysis, the strain was closely related to Nocardia niigatensis NBRC 100131T.
32897850	2	67	theme	soil	153:156	arg1	sample					158:163	a soil sample	151:163	a soil sample collected from Phichit Province, Thailand	151:205	A novel actinomycete strain, CT2-14T, belonging to the genus Nocardia, was isolated from a soil sample collected from Phichit Province, Thailand.
32897850	4	68	dep	optimum	321:327	arg1	28-37 °C					330:337	28-37 °C	330:337	28-37 °C	330:337	The strain grew at 15-40 °C (optimum, 28-37 °C), pH 6-11 (optimum, pH 6-8) and on an International Streptomyces Project 2 with 4 % (w/v) NaCl agar plate.
32897850	8	69	theme	summed	652:657	arg1	 0					648:649	 0	648:649	 0	648:649	The strain contained C16 : 0, summed feature 3, C17 : 0 10-methyl and C18 : 1 ω9c as the major fatty acids and MK-8(H4ω-cycl) as the major menaquinone.
32897850	8	69	theme	summed	652:657	arg1	feature					659:665	summed feature 3	652:667	summed feature 3	652:667	The strain contained C16 : 0, summed feature 3, C17 : 0 10-methyl and C18 : 1 ω9c as the major fatty acids and MK-8(H4ω-cycl) as the major menaquinone.
32897850	10	70	theme	highest	914:920	arg1	similarity					936:945	the highest 16S rRNA gene similarity	910:945	the highest 16S rRNA gene similarity to Nocardia veterana JCM 11307T (98.4 %), Nocardia africana JCM 11438T (98.2 %) and Nocardia kruczakiae JCM 13032T (98.0 %)	910:1069	Strain CT2-14T showed the highest 16S rRNA gene similarity to Nocardia veterana JCM 11307T (98.4 %), Nocardia africana JCM 11438T (98.2 %) and Nocardia kruczakiae JCM 13032T (98.0 %).
32897850	13	71	theme	genome	1330:1335	arg1	analyses					1337:1344	polyphasic and genome analyses	1315:1344	analyses	1337:1344	On the basis of polyphasic and genome analyses, strain CT2-14T represented a novel species of the genus Nocardia for which the name Nocardia aurantiaca sp.
32897850	8	72	contain	contained	633:641	arg1	strain					626:631	The strain	622:631	The strain	622:631	The strain contained C16 : 0, summed feature 3, C17 : 0 10-methyl and C18 : 1 ω9c as the major fatty acids and MK-8(H4ω-cycl) as the major menaquinone.
32897850	8	72	contain	contained	633:641	arg2	C16 					643:646	C16 	643:646	C16 : 0, summed feature 3, C17 : 0 10-methyl and C18 : 1 ω9c	643:702	The strain contained C16 : 0, summed feature 3, C17 : 0 10-methyl and C18 : 1 ω9c as the major fatty acids and MK-8(H4ω-cycl) as the major menaquinone.
32897850	8	72	contain	contained	633:641	arg2	acids					723:727	the major fatty acids	707:727	the major fatty acids	707:727	The strain contained C16 : 0, summed feature 3, C17 : 0 10-methyl and C18 : 1 ω9c as the major fatty acids and MK-8(H4ω-cycl) as the major menaquinone.
32897850	8	72	contain	contained	633:641	arg2	menaquinone					761:771	the major menaquinone	751:771	the major menaquinone	751:771	The strain contained C16 : 0, summed feature 3, C17 : 0 10-methyl and C18 : 1 ω9c as the major fatty acids and MK-8(H4ω-cycl) as the major menaquinone.
32897850	4	73	with	Project	404:410	arg1	plate					439:443	4 % (w/v) NaCl agar plate	419:443	4 % (w/v) NaCl agar plate	419:443	The strain grew at 15-40 °C (optimum, 28-37 °C), pH 6-11 (optimum, pH 6-8) and on an International Streptomyces Project 2 with 4 % (w/v) NaCl agar plate.
32897850	4	74	dep	optimum	350:356	arg1	pH					359:360	pH 6-8	359:364	pH 6-8	359:364	The strain grew at 15-40 °C (optimum, 28-37 °C), pH 6-11 (optimum, pH 6-8) and on an International Streptomyces Project 2 with 4 % (w/v) NaCl agar plate.
32897850	11	75	theme	G+C	1162:1164	arg1	content					1166:1172	an average G+C content	1151:1172	an average G+C content of 67.9 mol %	1151:1186	The draft genome of strain CT2-14T was 7.37 Mb with 6685 coding sequences with an average G+C content of 67.9 mol %.
32897850	10	76	theme	veterana	959:966	arg1	11307T					972:977	Nocardia veterana JCM 11307T	950:977	Nocardia veterana JCM 11307T (98.4 %)	950:986	Strain CT2-14T showed the highest 16S rRNA gene similarity to Nocardia veterana JCM 11307T (98.4 %), Nocardia africana JCM 11438T (98.2 %) and Nocardia kruczakiae JCM 13032T (98.0 %).
32897850	10	76	theme	veterana	959:966	arg1	%					985:985	98.4 %	980:985	98.4 %	980:985	Strain CT2-14T showed the highest 16S rRNA gene similarity to Nocardia veterana JCM 11307T (98.4 %), Nocardia africana JCM 11438T (98.2 %) and Nocardia kruczakiae JCM 13032T (98.0 %).
32897850	15	77	theme	33775T=TISTR	1506:1517	arg1	2838T					1519:1523	=JCM 33775T=TISTR 2838T	1501:1523	=JCM 33775T=TISTR 2838T	1501:1523	The type strain is CT2-14T (=JCM 33775T=TISTR 2838T).
32897850	15	77	theme	33775T=TISTR	1506:1517	arg1	CT2-14T					1492:1498	CT2-14T	1492:1498	CT2-14T (=JCM 33775T=TISTR 2838T)	1492:1524	The type strain is CT2-14T (=JCM 33775T=TISTR 2838T).
34932313	5	0	theme	mat	636:638	arg1	use					561:563	the use	557:563	the use of a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat (ENM) for the simultaneous enrichment and impedimetric sensing of ZIKV viral particles	557:725	Therefore, the authors propose the use of a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat (ENM) for the simultaneous enrichment and impedimetric sensing of ZIKV viral particles.
34932313	6	1	theme	blend	804:808	arg1	formulation					810:820	blend formulation	804:820	blend formulation	804:820	The electrospinning conditions of polyvinyl alcohol and alginate, including blend formulation, were optimized through a factorial design.
34932313	2	2	theme	viral	326:330	arg1	loads					332:336	low viral loads	322:336	low viral loads	322:336	After ZIKV infection, the blood, breast milk, and other body fluids contain low viral loads.
34932313	12	3	theme	UV-vis	1425:1430	arg1	spectroscopies					1462:1475	UV-vis and electrochemical impedance spectroscopies	1425:1475	Atomic force microscopy along with UV-vis and electrochemical impedance spectroscopies	1390:1475	Atomic force microscopy along with UV-vis and electrochemical impedance spectroscopies was used to monitor the antibody immobilization, ZIKV capture, and elution processes.
34932313	12	4	theme	impedance	1452:1460	arg1	spectroscopies					1462:1475	UV-vis and electrochemical impedance spectroscopies	1425:1475	Atomic force microscopy along with UV-vis and electrochemical impedance spectroscopies	1390:1475	Atomic force microscopy along with UV-vis and electrochemical impedance spectroscopies was used to monitor the antibody immobilization, ZIKV capture, and elution processes.
34932313	8	5	theme	in	1002:1003	arg1	deposition					1019:1028	in situ chemical deposition	1002:1028	in situ chemical deposition of conductive polyaniline	1002:1054	Hierarchization was achieved by in situ chemical deposition of conductive polyaniline.
34932313	12	6	theme	electrochemical	1436:1450	arg1	spectroscopies					1462:1475	UV-vis and electrochemical impedance spectroscopies	1425:1475	Atomic force microscopy along with UV-vis and electrochemical impedance spectroscopies	1390:1475	Atomic force microscopy along with UV-vis and electrochemical impedance spectroscopies was used to monitor the antibody immobilization, ZIKV capture, and elution processes.
34932313	13	7	with	μg	1634:1635	arg1	rate					1663:1666	a desorption rate	1650:1666	a desorption rate of 99.97% (38.29 ± 2.7 μg ZIKV), which is reusable for at least three times	1650:1742	Our results show that 14.2 mg (0.25 cm3) of ENM can capture 38.7 ± 2.5 μg of ZIKV with a desorption rate of 99.97% (38.29 ± 2.7 μg ZIKV), which is reusable for at least three times.
34932313	5	8	theme	electrospun	604:614	arg1	mat					636:638	a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat	568:638	a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat (ENM)	568:644	Therefore, the authors propose the use of a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat (ENM) for the simultaneous enrichment and impedimetric sensing of ZIKV viral particles.
34932313	5	8	theme	electrospun	604:614	arg1	ENM					641:643	ENM	641:643	ENM	641:643	Therefore, the authors propose the use of a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat (ENM) for the simultaneous enrichment and impedimetric sensing of ZIKV viral particles.
34932313	14	9	dep	capacity	1768:1775	arg1	micrograms					1778:1787	micrograms	1778:1787	micrograms of ZIKV captured per milligram of ENM	1778:1825	Therefore, the capture capacity (micrograms of ZIKV captured per milligram of ENM) of polyaniline-hierarchized mats was 2.72 μg ZIKV/mg.
34932313	1	10	theme	non-vector	163:172	arg1	transmission					185:196	non-vector congenital transmission	163:196	non-vector congenital transmission	163:196	Zika virus (ZIKV) infection is associated with the Guillain-Barré syndrome, and when non-vector congenital transmission occurs, fetal brain abnormalities are expected.
34932313	8	11	theme	chemical	1010:1017	arg1	deposition					1019:1028	in situ chemical deposition	1002:1028	in situ chemical deposition of conductive polyaniline	1002:1054	Hierarchization was achieved by in situ chemical deposition of conductive polyaniline.
34932313	12	12	theme	force	1397:1401	arg1	microscopy					1403:1412	Atomic force microscopy	1390:1412	Atomic force microscopy along with UV-vis and electrochemical impedance spectroscopies	1390:1475	Atomic force microscopy along with UV-vis and electrochemical impedance spectroscopies was used to monitor the antibody immobilization, ZIKV capture, and elution processes.
34932313	11	13	theme	electron	1360:1367	arg1	microscopy					1369:1378	scanning electron microscopy	1351:1378	scanning electron microscopy	1351:1378	ENMs were characterized by scanning electron microscopy and FTIR.
34932313	6	14	theme	polyvinyl	762:770	arg1	alcohol					772:778	polyvinyl alcohol	762:778	polyvinyl alcohol	762:778	The electrospinning conditions of polyvinyl alcohol and alginate, including blend formulation, were optimized through a factorial design.
34932313	9	15	theme	ZIKV	1201:1204	arg1	recognition					1186:1196	the specific recognition	1173:1196	the specific recognition of ZIKV within the medium of Vero cell culture	1173:1243	The carboxyl groups of the ENM were used for the covalent immobilization of anti-ZIKV polyclonal antibodies used in the specific recognition of ZIKV within the medium of Vero cell culture.
34932313	16	16	theme	purification	2030:2041	arg1	system					2043:2048	a fast small-scale purification system	2011:2048	a fast small-scale purification system that can simultaneously monitor ZIKV electrochemically and optically	2011:2117	As a result, we present a fast small-scale purification system that can simultaneously monitor ZIKV electrochemically and optically.
34932313	16	16	theme	purification	2030:2041	arg1	result					1992:1997	a result	1990:1997	a result	1990:1997	As a result, we present a fast small-scale purification system that can simultaneously monitor ZIKV electrochemically and optically.
34932313	14	17	theme	ENM	1823:1825	arg1	milligram					1810:1818	milligram	1810:1818	milligram of ENM	1810:1825	Therefore, the capture capacity (micrograms of ZIKV captured per milligram of ENM) of polyaniline-hierarchized mats was 2.72 μg ZIKV/mg.
34932313	9	18	used	used	1093:1096	arg2	ENM					1084:1086	the ENM	1080:1086	the ENM	1080:1086	The carboxyl groups of the ENM were used for the covalent immobilization of anti-ZIKV polyclonal antibodies used in the specific recognition of ZIKV within the medium of Vero cell culture.
34932313	9	18	used	used	1093:1096	arg2	groups					1070:1075	The carboxyl groups	1057:1075	The carboxyl groups of the ENM	1057:1086	The carboxyl groups of the ENM were used for the covalent immobilization of anti-ZIKV polyclonal antibodies used in the specific recognition of ZIKV within the medium of Vero cell culture.
34932313	1	19	theme	brain	212:216	arg1	abnormalities					218:230	fetal brain abnormalities	206:230	fetal brain abnormalities	206:230	Zika virus (ZIKV) infection is associated with the Guillain-Barré syndrome, and when non-vector congenital transmission occurs, fetal brain abnormalities are expected.
34932313	14	20	theme	captured	1797:1804	arg1	micrograms					1778:1787	micrograms	1778:1787	micrograms of ZIKV captured per milligram of ENM	1778:1825	Therefore, the capture capacity (micrograms of ZIKV captured per milligram of ENM) of polyaniline-hierarchized mats was 2.72 μg ZIKV/mg.
34932313	10	21	theme	specific	1250:1257	arg1	capture					1259:1265	specific capture	1250:1265	specific capture	1250:1265	The specific capture and desorption of virions were studied at different pHs.
34932313	5	22	theme	simultaneous	654:665	arg1	enrichment					667:676	simultaneous enrichment	654:676	simultaneous enrichment	654:676	Therefore, the authors propose the use of a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat (ENM) for the simultaneous enrichment and impedimetric sensing of ZIKV viral particles.
34932313	13	23	theme	ENM	1607:1609	arg1	mg					1590:1591	14.2 mg	1585:1591	14.2 mg (0.25 cm3) of ENM	1585:1609	Our results show that 14.2 mg (0.25 cm3) of ENM can capture 38.7 ± 2.5 μg of ZIKV with a desorption rate of 99.97% (38.29 ± 2.7 μg ZIKV), which is reusable for at least three times.
34932313	13	23	theme	ENM	1607:1609	arg1	cm3					1599:1601	0.25 cm3	1594:1601	0.25 cm3	1594:1601	Our results show that 14.2 mg (0.25 cm3) of ENM can capture 38.7 ± 2.5 μg of ZIKV with a desorption rate of 99.97% (38.29 ± 2.7 μg ZIKV), which is reusable for at least three times.
34932313	9	24	theme	carboxyl	1061:1068	arg1	groups					1070:1075	The carboxyl groups	1057:1075	The carboxyl groups of the ENM	1057:1086	The carboxyl groups of the ENM were used for the covalent immobilization of anti-ZIKV polyclonal antibodies used in the specific recognition of ZIKV within the medium of Vero cell culture.
34932313	9	24	theme	carboxyl	1061:1068	arg1	ENM					1084:1086	the ENM	1080:1086	the ENM	1080:1086	The carboxyl groups of the ENM were used for the covalent immobilization of anti-ZIKV polyclonal antibodies used in the specific recognition of ZIKV within the medium of Vero cell culture.
34932313	0	25	theme	Virus	71:75	arg1	Sensing					55:61	Impedimetric Sensing	42:61	Impedimetric Sensing of Zika Virus	42:75	Self-Enriching Electrospun Biosensors for Impedimetric Sensing of Zika Virus.
34932313	5	26	dep	enrichment	667:676	arg1	the					650:652	the	650:652	the	650:652	Therefore, the authors propose the use of a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat (ENM) for the simultaneous enrichment and impedimetric sensing of ZIKV viral particles.
34932313	4	27	theme	valuable	469:476	arg1	strategy					478:485	a valuable strategy	467:485	a valuable strategy to increase the analyte concentration	467:523	Pre-enrichment is a valuable strategy to increase the analyte concentration.
34932313	4	27	theme	valuable	469:476	arg1	Pre-enrichment					449:462	Pre-enrichment	449:462	Pre-enrichment	449:462	Pre-enrichment is a valuable strategy to increase the analyte concentration.
34932313	15	28	theme	LOD	1896:1898	arg1	μg					1932:1933	2.76 μg	1927:1933	2.76 μg of ZIKV particles (approximately 6.6 × 103 PFU/mL)	1927:1984	The impedance LOD value was determined to be 2.76 μg of ZIKV particles (approximately 6.6 × 103 PFU/mL).
34932313	15	28	theme	LOD	1896:1898	arg1	value					1900:1904	The impedance LOD value	1882:1904	The impedance LOD value	1882:1904	The impedance LOD value was determined to be 2.76 μg of ZIKV particles (approximately 6.6 × 103 PFU/mL).
34932313	2	29	contain	contain	314:320	arg2	loads					332:336	low viral loads	322:336	low viral loads	322:336	After ZIKV infection, the blood, breast milk, and other body fluids contain low viral loads.
34932313	2	29	contain	contain	314:320	arg1	blood					272:276	the blood	268:276	the blood	268:276	After ZIKV infection, the blood, breast milk, and other body fluids contain low viral loads.
34932313	2	29	contain	contain	314:320	arg1	milk					286:289	breast milk	279:289	breast milk	279:289	After ZIKV infection, the blood, breast milk, and other body fluids contain low viral loads.
34932313	2	29	contain	contain	314:320	arg1	fluids					307:312	other body fluids	296:312	other body fluids	296:312	After ZIKV infection, the blood, breast milk, and other body fluids contain low viral loads.
34932313	8	30	theme	polyaniline	1044:1054	arg1	deposition					1019:1028	in situ chemical deposition	1002:1028	in situ chemical deposition of conductive polyaniline	1002:1054	Hierarchization was achieved by in situ chemical deposition of conductive polyaniline.
34932313	14	31	theme	capture	1760:1766	arg1	ZIKV/mg					1873:1879	2.72 μg ZIKV/mg	1865:1879	2.72 μg ZIKV/mg	1865:1879	Therefore, the capture capacity (micrograms of ZIKV captured per milligram of ENM) of polyaniline-hierarchized mats was 2.72 μg ZIKV/mg.
34932313	14	31	theme	capture	1760:1766	arg1	capacity					1768:1775	the capture capacity	1756:1775	the capture capacity (micrograms of ZIKV captured per milligram of ENM) of polyaniline-hierarchized mats	1756:1859	Therefore, the capture capacity (micrograms of ZIKV captured per milligram of ENM) of polyaniline-hierarchized mats was 2.72 μg ZIKV/mg.
34932313	9	32	theme	ENM	1084:1086	arg1	groups					1070:1075	The carboxyl groups	1057:1075	The carboxyl groups of the ENM	1057:1086	The carboxyl groups of the ENM were used for the covalent immobilization of anti-ZIKV polyclonal antibodies used in the specific recognition of ZIKV within the medium of Vero cell culture.
34932313	9	32	theme	ENM	1084:1086	arg1	ENM					1084:1086	the ENM	1080:1086	the ENM	1080:1086	The carboxyl groups of the ENM were used for the covalent immobilization of anti-ZIKV polyclonal antibodies used in the specific recognition of ZIKV within the medium of Vero cell culture.
34932313	5	33	theme	ZIKV	706:709	arg1	particles					717:725	ZIKV viral particles	706:725	ZIKV viral particles	706:725	Therefore, the authors propose the use of a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat (ENM) for the simultaneous enrichment and impedimetric sensing of ZIKV viral particles.
34932313	5	34	theme	composite	581:589	arg1	mat					636:638	a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat	568:638	a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat (ENM)	568:644	Therefore, the authors propose the use of a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat (ENM) for the simultaneous enrichment and impedimetric sensing of ZIKV viral particles.
34932313	5	34	theme	composite	581:589	arg1	ENM					641:643	ENM	641:643	ENM	641:643	Therefore, the authors propose the use of a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat (ENM) for the simultaneous enrichment and impedimetric sensing of ZIKV viral particles.
34932313	3	35	theme	input	410:414	arg1	volumes					416:422	larger input volumes	403:422	larger input volumes of the clinical samples	403:446	Their detection is challenging as it requires the processing of larger input volumes of the clinical samples.
34932313	5	36	theme	particles	717:725	arg1	enrichment					667:676	simultaneous enrichment	654:676	simultaneous enrichment	654:676	Therefore, the authors propose the use of a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat (ENM) for the simultaneous enrichment and impedimetric sensing of ZIKV viral particles.
34932313	5	36	theme	particles	717:725	arg1	sensing					695:701	impedimetric sensing	682:701	impedimetric sensing	682:701	Therefore, the authors propose the use of a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat (ENM) for the simultaneous enrichment and impedimetric sensing of ZIKV viral particles.
34932313	1	37	theme	virus	83:87	arg1	infection					96:104	Zika virus (ZIKV) infection	78:104	Zika virus (ZIKV) infection	78:104	Zika virus (ZIKV) infection is associated with the Guillain-Barré syndrome, and when non-vector congenital transmission occurs, fetal brain abnormalities are expected.
34932313	0	38	theme	Self-Enriching	0:13	arg1	Electrospun					15:25	Self-Enriching Electrospun	0:25	Self-Enriching Electrospun	0:25	Self-Enriching Electrospun Biosensors for Impedimetric Sensing of Zika Virus.
34932313	10	39	dep	capture	1259:1265	arg1	The					1246:1248	The	1246:1248	The	1246:1248	The specific capture and desorption of virions were studied at different pHs.
34932313	13	40	theme	ZIKV	1640:1643	arg1	μg					1634:1635	38.7 ± 2.5 μg	1623:1635	38.7 ± 2.5 μg of ZIKV with a desorption rate of 99.97% (38.29 ± 2.7 μg ZIKV), which is reusable for at least three times	1623:1742	Our results show that 14.2 mg (0.25 cm3) of ENM can capture 38.7 ± 2.5 μg of ZIKV with a desorption rate of 99.97% (38.29 ± 2.7 μg ZIKV), which is reusable for at least three times.
34932313	8	41	dep	in	1002:1003	arg1	situ					1005:1008	situ	1005:1008	situ	1005:1008	Hierarchization was achieved by in situ chemical deposition of conductive polyaniline.
34932313	3	42	theme	clinical	431:438	arg1	samples					440:446	the clinical samples	427:446	the clinical samples	427:446	Their detection is challenging as it requires the processing of larger input volumes of the clinical samples.
34932313	0	43	theme	Impedimetric	42:53	arg1	Sensing					55:61	Impedimetric Sensing	42:61	Impedimetric Sensing of Zika Virus	42:75	Self-Enriching Electrospun Biosensors for Impedimetric Sensing of Zika Virus.
34932313	13	44	theme	±	1628:1628	arg1	μg					1634:1635	38.7 ± 2.5 μg	1623:1635	38.7 ± 2.5 μg of ZIKV with a desorption rate of 99.97% (38.29 ± 2.7 μg ZIKV), which is reusable for at least three times	1623:1742	Our results show that 14.2 mg (0.25 cm3) of ENM can capture 38.7 ± 2.5 μg of ZIKV with a desorption rate of 99.97% (38.29 ± 2.7 μg ZIKV), which is reusable for at least three times.
34932313	9	45	theme	antibodies	1154:1163	arg1	immobilization					1115:1128	the covalent immobilization	1102:1128	the covalent immobilization of anti-ZIKV polyclonal antibodies used in the specific recognition of ZIKV within the medium of Vero cell culture	1102:1243	The carboxyl groups of the ENM were used for the covalent immobilization of anti-ZIKV polyclonal antibodies used in the specific recognition of ZIKV within the medium of Vero cell culture.
34932313	12	46	dep	along	1414:1418	arg1	with					1420:1423	with	1420:1423	with	1420:1423	Atomic force microscopy along with UV-vis and electrochemical impedance spectroscopies was used to monitor the antibody immobilization, ZIKV capture, and elution processes.
34932313	12	47	theme	capture	1531:1537	arg1	processes					1552:1560	the antibody immobilization, ZIKV capture, and elution processes	1497:1560	the antibody immobilization, ZIKV capture, and elution processes	1497:1560	Atomic force microscopy along with UV-vis and electrochemical impedance spectroscopies was used to monitor the antibody immobilization, ZIKV capture, and elution processes.
34932313	9	48	theme	specific	1177:1184	arg1	recognition					1186:1196	the specific recognition	1173:1196	the specific recognition of ZIKV within the medium of Vero cell culture	1173:1243	The carboxyl groups of the ENM were used for the covalent immobilization of anti-ZIKV polyclonal antibodies used in the specific recognition of ZIKV within the medium of Vero cell culture.
34932313	14	49	theme	mats	1856:1859	arg1	ZIKV/mg					1873:1879	2.72 μg ZIKV/mg	1865:1879	2.72 μg ZIKV/mg	1865:1879	Therefore, the capture capacity (micrograms of ZIKV captured per milligram of ENM) of polyaniline-hierarchized mats was 2.72 μg ZIKV/mg.
34932313	14	49	theme	mats	1856:1859	arg1	capacity					1768:1775	the capture capacity	1756:1775	the capture capacity (micrograms of ZIKV captured per milligram of ENM) of polyaniline-hierarchized mats	1756:1859	Therefore, the capture capacity (micrograms of ZIKV captured per milligram of ENM) of polyaniline-hierarchized mats was 2.72 μg ZIKV/mg.
34932313	16	50	theme	small-scale	2018:2028	arg1	system					2043:2048	a fast small-scale purification system	2011:2048	a fast small-scale purification system that can simultaneously monitor ZIKV electrochemically and optically	2011:2117	As a result, we present a fast small-scale purification system that can simultaneously monitor ZIKV electrochemically and optically.
34932313	16	50	theme	small-scale	2018:2028	arg1	result					1992:1997	a result	1990:1997	a result	1990:1997	As a result, we present a fast small-scale purification system that can simultaneously monitor ZIKV electrochemically and optically.
34932313	5	51	theme	hydrogel	627:634	arg1	mat					636:638	a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat	568:638	a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat (ENM)	568:644	Therefore, the authors propose the use of a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat (ENM) for the simultaneous enrichment and impedimetric sensing of ZIKV viral particles.
34932313	5	51	theme	hydrogel	627:634	arg1	ENM					641:643	ENM	641:643	ENM	641:643	Therefore, the authors propose the use of a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat (ENM) for the simultaneous enrichment and impedimetric sensing of ZIKV viral particles.
34932313	12	52	theme	antibody	1501:1508	arg1	immobilization					1510:1523	antibody immobilization	1501:1523	antibody immobilization	1501:1523	Atomic force microscopy along with UV-vis and electrochemical impedance spectroscopies was used to monitor the antibody immobilization, ZIKV capture, and elution processes.
34932313	12	53	used	used	1481:1484	arg2	spectroscopies					1462:1475	UV-vis and electrochemical impedance spectroscopies	1425:1475	Atomic force microscopy along with UV-vis and electrochemical impedance spectroscopies	1390:1475	Atomic force microscopy along with UV-vis and electrochemical impedance spectroscopies was used to monitor the antibody immobilization, ZIKV capture, and elution processes.
34932313	12	53	used	used	1481:1484	arg2	microscopy					1403:1412	Atomic force microscopy	1390:1412	Atomic force microscopy along with UV-vis and electrochemical impedance spectroscopies	1390:1475	Atomic force microscopy along with UV-vis and electrochemical impedance spectroscopies was used to monitor the antibody immobilization, ZIKV capture, and elution processes.
34932313	9	54	theme	Vero	1227:1230	arg1	culture					1237:1243	Vero cell culture	1227:1243	Vero cell culture	1227:1243	The carboxyl groups of the ENM were used for the covalent immobilization of anti-ZIKV polyclonal antibodies used in the specific recognition of ZIKV within the medium of Vero cell culture.
34932313	2	55	theme	breast	279:284	arg1	milk					286:289	breast milk	279:289	breast milk	279:289	After ZIKV infection, the blood, breast milk, and other body fluids contain low viral loads.
34932313	6	56	theme	electrospinning	732:746	arg1	formulation					810:820	blend formulation	804:820	blend formulation	804:820	The electrospinning conditions of polyvinyl alcohol and alginate, including blend formulation, were optimized through a factorial design.
34932313	6	56	theme	electrospinning	732:746	arg1	conditions					748:757	The electrospinning conditions	728:757	The electrospinning conditions	728:757	The electrospinning conditions of polyvinyl alcohol and alginate, including blend formulation, were optimized through a factorial design.
34932313	2	57	theme	low	322:324	arg1	loads					332:336	low viral loads	322:336	low viral loads	322:336	After ZIKV infection, the blood, breast milk, and other body fluids contain low viral loads.
34932313	6	58	theme	alginate	784:791	arg1	formulation					810:820	blend formulation	804:820	blend formulation	804:820	The electrospinning conditions of polyvinyl alcohol and alginate, including blend formulation, were optimized through a factorial design.
34932313	6	58	theme	alginate	784:791	arg1	conditions					748:757	The electrospinning conditions	728:757	The electrospinning conditions	728:757	The electrospinning conditions of polyvinyl alcohol and alginate, including blend formulation, were optimized through a factorial design.
34932313	14	59	theme	polyaniline-hierarchized	1831:1854	arg1	mats					1856:1859	polyaniline-hierarchized mats	1831:1859	polyaniline-hierarchized mats	1831:1859	Therefore, the capture capacity (micrograms of ZIKV captured per milligram of ENM) of polyaniline-hierarchized mats was 2.72 μg ZIKV/mg.
34932313	16	60	theme	fast	2013:2016	arg1	system					2043:2048	a fast small-scale purification system	2011:2048	a fast small-scale purification system that can simultaneously monitor ZIKV electrochemically and optically	2011:2117	As a result, we present a fast small-scale purification system that can simultaneously monitor ZIKV electrochemically and optically.
34932313	16	60	theme	fast	2013:2016	arg1	result					1992:1997	a result	1990:1997	a result	1990:1997	As a result, we present a fast small-scale purification system that can simultaneously monitor ZIKV electrochemically and optically.
34932313	11	61	theme	scanning	1351:1358	arg1	microscopy					1369:1378	scanning electron microscopy	1351:1378	scanning electron microscopy	1351:1378	ENMs were characterized by scanning electron microscopy and FTIR.
34932313	5	62	theme	nanofiber	616:624	arg1	mat					636:638	a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat	568:638	a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat (ENM)	568:644	Therefore, the authors propose the use of a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat (ENM) for the simultaneous enrichment and impedimetric sensing of ZIKV viral particles.
34932313	5	62	theme	nanofiber	616:624	arg1	ENM					641:643	ENM	641:643	ENM	641:643	Therefore, the authors propose the use of a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat (ENM) for the simultaneous enrichment and impedimetric sensing of ZIKV viral particles.
34932313	6	63	theme	alcohol	772:778	arg1	formulation					810:820	blend formulation	804:820	blend formulation	804:820	The electrospinning conditions of polyvinyl alcohol and alginate, including blend formulation, were optimized through a factorial design.
34932313	6	63	theme	alcohol	772:778	arg1	conditions					748:757	The electrospinning conditions	728:757	The electrospinning conditions	728:757	The electrospinning conditions of polyvinyl alcohol and alginate, including blend formulation, were optimized through a factorial design.
34932313	2	64	theme	other	296:300	arg1	fluids					307:312	other body fluids	296:312	other body fluids	296:312	After ZIKV infection, the blood, breast milk, and other body fluids contain low viral loads.
34932313	1	65	theme	congenital	174:183	arg1	transmission					185:196	non-vector congenital transmission	163:196	non-vector congenital transmission	163:196	Zika virus (ZIKV) infection is associated with the Guillain-Barré syndrome, and when non-vector congenital transmission occurs, fetal brain abnormalities are expected.
34932313	8	66	theme	conductive	1033:1042	arg1	polyaniline					1044:1054	conductive polyaniline	1033:1054	conductive polyaniline	1033:1054	Hierarchization was achieved by in situ chemical deposition of conductive polyaniline.
34932313	12	67	theme	Atomic	1390:1395	arg1	microscopy					1403:1412	Atomic force microscopy	1390:1412	Atomic force microscopy along with UV-vis and electrochemical impedance spectroscopies	1390:1475	Atomic force microscopy along with UV-vis and electrochemical impedance spectroscopies was used to monitor the antibody immobilization, ZIKV capture, and elution processes.
34932313	9	68	theme	cell	1232:1235	arg1	culture					1237:1243	Vero cell culture	1227:1243	Vero cell culture	1227:1243	The carboxyl groups of the ENM were used for the covalent immobilization of anti-ZIKV polyclonal antibodies used in the specific recognition of ZIKV within the medium of Vero cell culture.
34932313	9	69	theme	culture	1237:1243	arg1	medium					1217:1222	the medium	1213:1222	the medium of Vero cell culture	1213:1243	The carboxyl groups of the ENM were used for the covalent immobilization of anti-ZIKV polyclonal antibodies used in the specific recognition of ZIKV within the medium of Vero cell culture.
34932313	5	70	theme	impedimetric	682:693	arg1	sensing					695:701	impedimetric sensing	682:701	impedimetric sensing	682:701	Therefore, the authors propose the use of a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat (ENM) for the simultaneous enrichment and impedimetric sensing of ZIKV viral particles.
34932313	1	71	theme	fetal	206:210	arg1	abnormalities					218:230	fetal brain abnormalities	206:230	fetal brain abnormalities	206:230	Zika virus (ZIKV) infection is associated with the Guillain-Barré syndrome, and when non-vector congenital transmission occurs, fetal brain abnormalities are expected.
34932313	14	72	theme	ZIKV	1792:1795	arg1	captured					1797:1804	ZIKV captured	1792:1804	ZIKV captured	1792:1804	Therefore, the capture capacity (micrograms of ZIKV captured per milligram of ENM) of polyaniline-hierarchized mats was 2.72 μg ZIKV/mg.
34932313	2	73	theme	ZIKV	252:255	arg1	infection					257:265	ZIKV infection	252:265	ZIKV infection	252:265	After ZIKV infection, the blood, breast milk, and other body fluids contain low viral loads.
34932313	1	74	theme	Guillain-Barré	129:142	arg1	syndrome					144:151	the Guillain-Barré syndrome	125:151	the Guillain-Barré syndrome	125:151	Zika virus (ZIKV) infection is associated with the Guillain-Barré syndrome, and when non-vector congenital transmission occurs, fetal brain abnormalities are expected.
34932313	10	75	theme	virions	1285:1291	arg1	desorption					1271:1280	desorption	1271:1280	desorption	1271:1280	The specific capture and desorption of virions were studied at different pHs.
34932313	10	75	theme	virions	1285:1291	arg1	capture					1259:1265	specific capture	1250:1265	specific capture	1250:1265	The specific capture and desorption of virions were studied at different pHs.
34932313	5	76	theme	hierarchal	570:579	arg1	mat					636:638	a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat	568:638	a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat (ENM)	568:644	Therefore, the authors propose the use of a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat (ENM) for the simultaneous enrichment and impedimetric sensing of ZIKV viral particles.
34932313	5	76	theme	hierarchal	570:579	arg1	ENM					641:643	ENM	641:643	ENM	641:643	Therefore, the authors propose the use of a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat (ENM) for the simultaneous enrichment and impedimetric sensing of ZIKV viral particles.
34932313	15	77	theme	impedance	1886:1894	arg1	μg					1932:1933	2.76 μg	1927:1933	2.76 μg of ZIKV particles (approximately 6.6 × 103 PFU/mL)	1927:1984	The impedance LOD value was determined to be 2.76 μg of ZIKV particles (approximately 6.6 × 103 PFU/mL).
34932313	15	77	theme	impedance	1886:1894	arg1	value					1900:1904	The impedance LOD value	1882:1904	The impedance LOD value	1882:1904	The impedance LOD value was determined to be 2.76 μg of ZIKV particles (approximately 6.6 × 103 PFU/mL).
34932313	10	78	theme	different	1309:1317	arg1	pHs					1319:1321	different pHs	1309:1321	different pHs	1309:1321	The specific capture and desorption of virions were studied at different pHs.
34932313	5	79	theme	viral	711:715	arg1	particles					717:725	ZIKV viral particles	706:725	ZIKV viral particles	706:725	Therefore, the authors propose the use of a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat (ENM) for the simultaneous enrichment and impedimetric sensing of ZIKV viral particles.
34932313	5	80	theme	polyaniline-	591:602	arg1	mat					636:638	a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat	568:638	a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat (ENM)	568:644	Therefore, the authors propose the use of a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat (ENM) for the simultaneous enrichment and impedimetric sensing of ZIKV viral particles.
34932313	5	80	theme	polyaniline-	591:602	arg1	ENM					641:643	ENM	641:643	ENM	641:643	Therefore, the authors propose the use of a hierarchal composite polyaniline-(electrospun nanofiber) hydrogel mat (ENM) for the simultaneous enrichment and impedimetric sensing of ZIKV viral particles.
34932313	1	81	theme	Zika	78:81	arg1	ZIKV					90:93	ZIKV	90:93	ZIKV	90:93	Zika virus (ZIKV) infection is associated with the Guillain-Barré syndrome, and when non-vector congenital transmission occurs, fetal brain abnormalities are expected.
34932313	1	81	theme	Zika	78:81	arg1	virus					83:87	Zika virus	78:87	Zika virus (ZIKV) infection	78:104	Zika virus (ZIKV) infection is associated with the Guillain-Barré syndrome, and when non-vector congenital transmission occurs, fetal brain abnormalities are expected.
34932313	3	82	theme	volumes	416:422	arg1	processing					389:398	the processing	385:398	the processing of larger input volumes of the clinical samples	385:446	Their detection is challenging as it requires the processing of larger input volumes of the clinical samples.
34932313	7	83	theme	hydrogel	949:956	arg1	properties					958:967	the hydrogel properties	945:967	the hydrogel properties	945:967	Disintegration and gelatinization were controlled via cross-linking to improve the hydrogel properties.
34932313	13	84	theme	%	1676:1676	arg1	rate					1663:1666	a desorption rate	1650:1666	a desorption rate of 99.97% (38.29 ± 2.7 μg ZIKV), which is reusable for at least three times	1650:1742	Our results show that 14.2 mg (0.25 cm3) of ENM can capture 38.7 ± 2.5 μg of ZIKV with a desorption rate of 99.97% (38.29 ± 2.7 μg ZIKV), which is reusable for at least three times.
34932313	9	85	theme	covalent	1106:1113	arg1	immobilization					1115:1128	the covalent immobilization	1102:1128	the covalent immobilization of anti-ZIKV polyclonal antibodies used in the specific recognition of ZIKV within the medium of Vero cell culture	1102:1243	The carboxyl groups of the ENM were used for the covalent immobilization of anti-ZIKV polyclonal antibodies used in the specific recognition of ZIKV within the medium of Vero cell culture.
34932313	3	86	theme	larger	403:408	arg1	volumes					416:422	larger input volumes	403:422	larger input volumes of the clinical samples	403:446	Their detection is challenging as it requires the processing of larger input volumes of the clinical samples.
34932313	15	87	theme	particles	1943:1951	arg1	μg					1932:1933	2.76 μg	1927:1933	2.76 μg of ZIKV particles (approximately 6.6 × 103 PFU/mL)	1927:1984	The impedance LOD value was determined to be 2.76 μg of ZIKV particles (approximately 6.6 × 103 PFU/mL).
34932313	15	87	theme	particles	1943:1951	arg1	value					1900:1904	The impedance LOD value	1882:1904	The impedance LOD value	1882:1904	The impedance LOD value was determined to be 2.76 μg of ZIKV particles (approximately 6.6 × 103 PFU/mL).
34932313	15	88	theme	ZIKV	1938:1941	arg1	particles					1943:1951	ZIKV particles	1938:1951	ZIKV particles (approximately 6.6 × 103 PFU/mL)	1938:1984	The impedance LOD value was determined to be 2.76 μg of ZIKV particles (approximately 6.6 × 103 PFU/mL).
34932313	15	88	theme	ZIKV	1938:1941	arg1	PFU/mL					1978:1983	approximately 6.6 × 103 PFU/mL	1954:1983	approximately 6.6 × 103 PFU/mL	1954:1983	The impedance LOD value was determined to be 2.76 μg of ZIKV particles (approximately 6.6 × 103 PFU/mL).
34932313	3	89	theme	samples	440:446	arg1	volumes					416:422	larger input volumes	403:422	larger input volumes of the clinical samples	403:446	Their detection is challenging as it requires the processing of larger input volumes of the clinical samples.
34932313	6	90	theme	factorial	848:856	arg1	design					858:863	a factorial design	846:863	a factorial design	846:863	The electrospinning conditions of polyvinyl alcohol and alginate, including blend formulation, were optimized through a factorial design.
34932313	4	91	theme	analyte	503:509	arg1	concentration					511:523	the analyte concentration	499:523	the analyte concentration	499:523	Pre-enrichment is a valuable strategy to increase the analyte concentration.
34932313	13	92	theme	desorption	1652:1661	arg1	rate					1663:1666	a desorption rate	1650:1666	a desorption rate of 99.97% (38.29 ± 2.7 μg ZIKV), which is reusable for at least three times	1650:1742	Our results show that 14.2 mg (0.25 cm3) of ENM can capture 38.7 ± 2.5 μg of ZIKV with a desorption rate of 99.97% (38.29 ± 2.7 μg ZIKV), which is reusable for at least three times.
34932313	9	93	theme	polyclonal	1143:1152	arg1	antibodies					1154:1163	anti-ZIKV polyclonal antibodies	1133:1163	anti-ZIKV polyclonal antibodies used in the specific recognition of ZIKV within the medium of Vero cell culture	1133:1243	The carboxyl groups of the ENM were used for the covalent immobilization of anti-ZIKV polyclonal antibodies used in the specific recognition of ZIKV within the medium of Vero cell culture.
34932313	0	94	theme	Zika	66:69	arg1	Virus					71:75	Zika Virus	66:75	Zika Virus	66:75	Self-Enriching Electrospun Biosensors for Impedimetric Sensing of Zika Virus.
34932313	12	95	theme	elution	1544:1550	arg1	processes					1552:1560	the antibody immobilization, ZIKV capture, and elution processes	1497:1560	the antibody immobilization, ZIKV capture, and elution processes	1497:1560	Atomic force microscopy along with UV-vis and electrochemical impedance spectroscopies was used to monitor the antibody immobilization, ZIKV capture, and elution processes.
34932313	12	96	theme	immobilization	1510:1523	arg1	processes					1552:1560	the antibody immobilization, ZIKV capture, and elution processes	1497:1560	the antibody immobilization, ZIKV capture, and elution processes	1497:1560	Atomic force microscopy along with UV-vis and electrochemical impedance spectroscopies was used to monitor the antibody immobilization, ZIKV capture, and elution processes.
34932313	2	97	theme	body	302:305	arg1	fluids					307:312	other body fluids	296:312	other body fluids	296:312	After ZIKV infection, the blood, breast milk, and other body fluids contain low viral loads.
34932313	12	98	theme	ZIKV	1526:1529	arg1	capture					1531:1537	ZIKV capture	1526:1537	ZIKV capture	1526:1537	Atomic force microscopy along with UV-vis and electrochemical impedance spectroscopies was used to monitor the antibody immobilization, ZIKV capture, and elution processes.
34932313	14	99	theme	μg	1870:1871	arg1	ZIKV/mg					1873:1879	2.72 μg ZIKV/mg	1865:1879	2.72 μg ZIKV/mg	1865:1879	Therefore, the capture capacity (micrograms of ZIKV captured per milligram of ENM) of polyaniline-hierarchized mats was 2.72 μg ZIKV/mg.
34932313	14	99	theme	μg	1870:1871	arg1	capacity					1768:1775	the capture capacity	1756:1775	the capture capacity (micrograms of ZIKV captured per milligram of ENM) of polyaniline-hierarchized mats	1756:1859	Therefore, the capture capacity (micrograms of ZIKV captured per milligram of ENM) of polyaniline-hierarchized mats was 2.72 μg ZIKV/mg.
34522610	5	0	theme	metabolites	690:700	arg1	metabolites					690:700	eight identified inhibitory metabolites	662:700	eight identified inhibitory metabolites	662:700	Six out of eight identified inhibitory metabolites, caused by the inefficient cell metabolism, were not previously studied in CHO cells: aconitic acid, 2-hydroxyisocaproic acid, methylsuccinic acid, cytidine monophosphate, trigonelline, and n-acetyl putrescine.
34522610	5	0	theme	metabolites	690:700	arg1	Six					651:653	Six	651:653	Six	651:653	Six out of eight identified inhibitory metabolites, caused by the inefficient cell metabolism, were not previously studied in CHO cells: aconitic acid, 2-hydroxyisocaproic acid, methylsuccinic acid, cytidine monophosphate, trigonelline, and n-acetyl putrescine.
34522610	8	1	theme	amino	1717:1721	arg1	tryptophan					1730:1739	tryptophan	1730:1739	tryptophan	1730:1739	In-depth pathway analysis revealed that these metabolites are produced when cells utilize major energy sources such as glucose and select amino acids (tryptophan, arginine, isoleucine, and leucine) for growth, maintenance, and protein production.
34522610	8	1	theme	amino	1717:1721	arg1	acids					1723:1727	select amino acids	1710:1727	select amino acids (tryptophan, arginine, isoleucine, and leucine)	1710:1775	In-depth pathway analysis revealed that these metabolites are produced when cells utilize major energy sources such as glucose and select amino acids (tryptophan, arginine, isoleucine, and leucine) for growth, maintenance, and protein production.
34522610	8	1	theme	amino	1717:1721	arg1	isoleucine					1752:1761	isoleucine	1752:1761	isoleucine	1752:1761	In-depth pathway analysis revealed that these metabolites are produced when cells utilize major energy sources such as glucose and select amino acids (tryptophan, arginine, isoleucine, and leucine) for growth, maintenance, and protein production.
34522610	8	1	theme	amino	1717:1721	arg1	leucine					1768:1774	leucine	1768:1774	leucine	1768:1774	In-depth pathway analysis revealed that these metabolites are produced when cells utilize major energy sources such as glucose and select amino acids (tryptophan, arginine, isoleucine, and leucine) for growth, maintenance, and protein production.
34522610	8	1	theme	amino	1717:1721	arg1	arginine					1742:1749	arginine	1742:1749	arginine	1742:1749	In-depth pathway analysis revealed that these metabolites are produced when cells utilize major energy sources such as glucose and select amino acids (tryptophan, arginine, isoleucine, and leucine) for growth, maintenance, and protein production.
34522610	6	2	theme	biantennary	1307:1317	arg1	N-glycans					1340:1348	mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans	1265:1348	N-glycans	1340:1348	When supplemented back into a fed-batch culture, significant reduction in cellular growth was observed in the presence of each metabolite and all the identified metabolites were shown to impact the glycosylation of a model secreted antibody, with seven of these also reducing CHO cellular productivity (titer) and all eight inhibiting the formation of mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans.
34522610	7	3	theme	fed-batch	1507:1515	arg1	culture					1517:1523	fed-batch culture	1507:1523	fed-batch culture	1507:1523	These inhibitory metabolites further impact the metabolism of cells, leading to a significant reduction in CHO cellular growth and specific productivity in fed-batch culture (maximum reductions of 27.2% and 40.6% respectively).
34522610	7	4	theme	CHO	1458:1460	arg1	growth					1471:1476	CHO cellular growth	1458:1476	CHO cellular growth	1458:1476	These inhibitory metabolites further impact the metabolism of cells, leading to a significant reduction in CHO cellular growth and specific productivity in fed-batch culture (maximum reductions of 27.2% and 40.6% respectively).
34522610	9	5	theme	multiple	1905:1912	arg1	lines					1923:1927	multiple CHO cell lines	1905:1927	multiple CHO cell lines (CHO-K1 and CHO-GS) as well as HEK293 cell line	1905:1975	Furthermore, these novel inhibitory metabolites were observed to accumulate in multiple CHO cell lines (CHO-K1 and CHO-GS) as well as HEK293 cell line.
34522610	9	5	theme	multiple	1905:1912	arg1	CHO-GS					1941:1946	CHO-GS	1941:1946	CHO-GS	1941:1946	Furthermore, these novel inhibitory metabolites were observed to accumulate in multiple CHO cell lines (CHO-K1 and CHO-GS) as well as HEK293 cell line.
34522610	9	5	theme	multiple	1905:1912	arg1	CHO-K1					1930:1935	CHO-K1	1930:1935	CHO-K1	1930:1935	Furthermore, these novel inhibitory metabolites were observed to accumulate in multiple CHO cell lines (CHO-K1 and CHO-GS) as well as HEK293 cell line.
34522610	8	6	theme	major	1669:1673	arg1	acids					1723:1727	select amino acids	1710:1727	select amino acids (tryptophan, arginine, isoleucine, and leucine)	1710:1775	In-depth pathway analysis revealed that these metabolites are produced when cells utilize major energy sources such as glucose and select amino acids (tryptophan, arginine, isoleucine, and leucine) for growth, maintenance, and protein production.
34522610	8	6	theme	major	1669:1673	arg1	sources					1682:1688	major energy sources	1669:1688	major energy sources such as glucose and select amino acids (tryptophan, arginine, isoleucine, and leucine) for growth, maintenance, and protein production	1669:1823	In-depth pathway analysis revealed that these metabolites are produced when cells utilize major energy sources such as glucose and select amino acids (tryptophan, arginine, isoleucine, and leucine) for growth, maintenance, and protein production.
34522610	8	6	theme	major	1669:1673	arg1	glucose					1698:1704	glucose	1698:1704	glucose	1698:1704	In-depth pathway analysis revealed that these metabolites are produced when cells utilize major energy sources such as glucose and select amino acids (tryptophan, arginine, isoleucine, and leucine) for growth, maintenance, and protein production.
34522610	6	7	theme	G1F	1298:1300	arg1	formation					1252:1260	the formation	1248:1260	the formation of mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans	1248:1348	When supplemented back into a fed-batch culture, significant reduction in cellular growth was observed in the presence of each metabolite and all the identified metabolites were shown to impact the glycosylation of a model secreted antibody, with seven of these also reducing CHO cellular productivity (titer) and all eight inhibiting the formation of mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans.
34522610	6	8	theme	cellular	1193:1200	arg1	titer					1216:1220	titer	1216:1220	titer	1216:1220	When supplemented back into a fed-batch culture, significant reduction in cellular growth was observed in the presence of each metabolite and all the identified metabolites were shown to impact the glycosylation of a model secreted antibody, with seven of these also reducing CHO cellular productivity (titer) and all eight inhibiting the formation of mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans.
34522610	6	8	theme	cellular	1193:1200	arg1	productivity					1202:1213	CHO cellular productivity	1189:1213	CHO cellular productivity (titer)	1189:1221	When supplemented back into a fed-batch culture, significant reduction in cellular growth was observed in the presence of each metabolite and all the identified metabolites were shown to impact the glycosylation of a model secreted antibody, with seven of these also reducing CHO cellular productivity (titer) and all eight inhibiting the formation of mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans.
34522610	9	9	theme	cell	1918:1921	arg1	lines					1923:1927	multiple CHO cell lines	1905:1927	multiple CHO cell lines (CHO-K1 and CHO-GS) as well as HEK293 cell line	1905:1975	Furthermore, these novel inhibitory metabolites were observed to accumulate in multiple CHO cell lines (CHO-K1 and CHO-GS) as well as HEK293 cell line.
34522610	9	9	theme	cell	1918:1921	arg1	CHO-GS					1941:1946	CHO-GS	1941:1946	CHO-GS	1941:1946	Furthermore, these novel inhibitory metabolites were observed to accumulate in multiple CHO cell lines (CHO-K1 and CHO-GS) as well as HEK293 cell line.
34522610	9	9	theme	cell	1918:1921	arg1	CHO-K1					1930:1935	CHO-K1	1930:1935	CHO-K1	1930:1935	Furthermore, these novel inhibitory metabolites were observed to accumulate in multiple CHO cell lines (CHO-K1 and CHO-GS) as well as HEK293 cell line.
34522610	6	10	theme	biantennary	1285:1295	arg1	G1F					1298:1300	mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans	1265:1348	G1F	1298:1300	When supplemented back into a fed-batch culture, significant reduction in cellular growth was observed in the presence of each metabolite and all the identified metabolites were shown to impact the glycosylation of a model secreted antibody, with seven of these also reducing CHO cellular productivity (titer) and all eight inhibiting the formation of mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans.
34522610	5	11	theme	n-acetyl	892:899	arg1	putrescine					901:910	n-acetyl putrescine	892:910	n-acetyl putrescine	892:910	Six out of eight identified inhibitory metabolites, caused by the inefficient cell metabolism, were not previously studied in CHO cells: aconitic acid, 2-hydroxyisocaproic acid, methylsuccinic acid, cytidine monophosphate, trigonelline, and n-acetyl putrescine.
34522610	5	12	theme	identified	668:677	arg1	metabolites					690:700	eight identified inhibitory metabolites	662:700	eight identified inhibitory metabolites	662:700	Six out of eight identified inhibitory metabolites, caused by the inefficient cell metabolism, were not previously studied in CHO cells: aconitic acid, 2-hydroxyisocaproic acid, methylsuccinic acid, cytidine monophosphate, trigonelline, and n-acetyl putrescine.
34522610	6	13	theme	these	1169:1173	arg1	these					1169:1173	these	1169:1173	these	1169:1173	When supplemented back into a fed-batch culture, significant reduction in cellular growth was observed in the presence of each metabolite and all the identified metabolites were shown to impact the glycosylation of a model secreted antibody, with seven of these also reducing CHO cellular productivity (titer) and all eight inhibiting the formation of mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans.
34522610	6	13	theme	these	1169:1173	arg1	seven					1160:1164	seven	1160:1164	seven	1160:1164	When supplemented back into a fed-batch culture, significant reduction in cellular growth was observed in the presence of each metabolite and all the identified metabolites were shown to impact the glycosylation of a model secreted antibody, with seven of these also reducing CHO cellular productivity (titer) and all eight inhibiting the formation of mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans.
34522610	10	14	theme	metabolites	2154:2164	arg1	elucidation					2105:2115	elucidation	2105:2115	elucidation	2105:2115	This study provides a robust and holistic methodology to incorporate global metabolomic analysis into cell culture studies for elucidation and structural verification of novel metabolites that participate in key metabolic pathways to growth, production, and post-translational modification in biopharmaceutical production.
34522610	10	14	theme	metabolites	2154:2164	arg1	verification					2132:2143	structural verification	2121:2143	structural verification	2121:2143	This study provides a robust and holistic methodology to incorporate global metabolomic analysis into cell culture studies for elucidation and structural verification of novel metabolites that participate in key metabolic pathways to growth, production, and post-translational modification in biopharmaceutical production.
34522610	7	15	theme	cells	1413:1417	arg1	metabolism					1399:1408	the metabolism	1395:1408	the metabolism of cells, leading to a significant reduction in CHO cellular growth and specific productivity	1395:1502	These inhibitory metabolites further impact the metabolism of cells, leading to a significant reduction in CHO cellular growth and specific productivity in fed-batch culture (maximum reductions of 27.2% and 40.6% respectively).
34522610	6	16	theme	N-glycans	1340:1348	arg1	formation					1252:1260	the formation	1248:1260	the formation of mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans	1248:1348	When supplemented back into a fed-batch culture, significant reduction in cellular growth was observed in the presence of each metabolite and all the identified metabolites were shown to impact the glycosylation of a model secreted antibody, with seven of these also reducing CHO cellular productivity (titer) and all eight inhibiting the formation of mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans.
34522610	9	17	dep	lines	1923:1927	arg1	lines					1923:1927	multiple CHO cell lines	1905:1927	multiple CHO cell lines (CHO-K1 and CHO-GS) as well as HEK293 cell line	1905:1975	Furthermore, these novel inhibitory metabolites were observed to accumulate in multiple CHO cell lines (CHO-K1 and CHO-GS) as well as HEK293 cell line.
34522610	9	17	dep	lines	1923:1927	arg1	CHO-GS					1941:1946	CHO-GS	1941:1946	CHO-GS	1941:1946	Furthermore, these novel inhibitory metabolites were observed to accumulate in multiple CHO cell lines (CHO-K1 and CHO-GS) as well as HEK293 cell line.
34522610	9	17	dep	lines	1923:1927	arg1	CHO-K1					1930:1935	CHO-K1	1930:1935	CHO-K1	1930:1935	Furthermore, these novel inhibitory metabolites were observed to accumulate in multiple CHO cell lines (CHO-K1 and CHO-GS) as well as HEK293 cell line.
34522610	10	18	theme	key	2186:2188	arg1	pathways					2200:2207	key metabolic pathways	2186:2207	key metabolic pathways to growth, production, and post-translational modification in biopharmaceutical production	2186:2298	This study provides a robust and holistic methodology to incorporate global metabolomic analysis into cell culture studies for elucidation and structural verification of novel metabolites that participate in key metabolic pathways to growth, production, and post-translational modification in biopharmaceutical production.
34522610	6	19	theme	G2F	1335:1337	arg1	N-glycans					1340:1348	mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans	1265:1348	N-glycans	1340:1348	When supplemented back into a fed-batch culture, significant reduction in cellular growth was observed in the presence of each metabolite and all the identified metabolites were shown to impact the glycosylation of a model secreted antibody, with seven of these also reducing CHO cellular productivity (titer) and all eight inhibiting the formation of mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans.
34522610	2	20	theme	metabolic	223:231	arg1	inefficiencies					233:246	endogenous metabolic inefficiencies	212:246	endogenous metabolic inefficiencies	212:246	However, endogenous metabolic inefficiencies often prevent cells to fully utilize nutrients to support growth and protein production.
34522610	1	21	theme	Mammalian	123:131	arg1	cells					133:137	Mammalian cells	123:137	Mammalian cells	123:137	Mammalian cells consume large amount of nutrients during growth and production.
34522610	6	22	theme	significant	962:972	arg1	reduction					974:982	significant reduction	962:982	significant reduction in cellular growth	962:1001	When supplemented back into a fed-batch culture, significant reduction in cellular growth was observed in the presence of each metabolite and all the identified metabolites were shown to impact the glycosylation of a model secreted antibody, with seven of these also reducing CHO cellular productivity (titer) and all eight inhibiting the formation of mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans.
34522610	10	23	theme	structural	2121:2130	arg1	verification					2132:2143	structural verification	2121:2143	structural verification	2121:2143	This study provides a robust and holistic methodology to incorporate global metabolomic analysis into cell culture studies for elucidation and structural verification of novel metabolites that participate in key metabolic pathways to growth, production, and post-translational modification in biopharmaceutical production.
34522610	9	24	theme	HEK293 cell	1960:1970	arg1	line					1972:1975	HEK293 cell line	1960:1975	multiple CHO cell lines (CHO-K1 and CHO-GS) as well as HEK293 cell line	1905:1975	Furthermore, these novel inhibitory metabolites were observed to accumulate in multiple CHO cell lines (CHO-K1 and CHO-GS) as well as HEK293 cell line.
34522610	6	25	theme	galactosylated	1319:1332	arg1	N-glycans					1340:1348	mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans	1265:1348	N-glycans	1340:1348	When supplemented back into a fed-batch culture, significant reduction in cellular growth was observed in the presence of each metabolite and all the identified metabolites were shown to impact the glycosylation of a model secreted antibody, with seven of these also reducing CHO cellular productivity (titer) and all eight inhibiting the formation of mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans.
34522610	3	26	theme	significant	346:356	arg1	fraction					358:365	significant fraction	346:365	significant fraction of fed nutrients	346:382	Instead, significant fraction of fed nutrients is diverted into extracellular accumulation of waste by-products and metabolites, further inhibiting proliferation and protein synthesis.
34522610	0	27	from	synthesis	93:101	arg1	cells					116:120	mammalian cells	106:120	mammalian cells	106:120	Identification of novel inhibitory metabolites and impact verification on growth and protein synthesis in mammalian cells.
34522610	5	28	theme	cytidine	850:857	arg1	monophosphate					859:871	cytidine monophosphate	850:871	cytidine monophosphate	850:871	Six out of eight identified inhibitory metabolites, caused by the inefficient cell metabolism, were not previously studied in CHO cells: aconitic acid, 2-hydroxyisocaproic acid, methylsuccinic acid, cytidine monophosphate, trigonelline, and n-acetyl putrescine.
34522610	7	29	from	reduction	1445:1453	arg1	growth					1471:1476	CHO cellular growth	1458:1476	CHO cellular growth	1458:1476	These inhibitory metabolites further impact the metabolism of cells, leading to a significant reduction in CHO cellular growth and specific productivity in fed-batch culture (maximum reductions of 27.2% and 40.6% respectively).
34522610	7	29	from	reduction	1445:1453	arg1	productivity					1491:1502	specific productivity	1482:1502	specific productivity	1482:1502	These inhibitory metabolites further impact the metabolism of cells, leading to a significant reduction in CHO cellular growth and specific productivity in fed-batch culture (maximum reductions of 27.2% and 40.6% respectively).
34522610	6	30	theme	metabolite	1040:1049	arg1	presence					1023:1030	the presence	1019:1030	the presence of each metabolite	1019:1049	When supplemented back into a fed-batch culture, significant reduction in cellular growth was observed in the presence of each metabolite and all the identified metabolites were shown to impact the glycosylation of a model secreted antibody, with seven of these also reducing CHO cellular productivity (titer) and all eight inhibiting the formation of mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans.
34522610	8	31	theme	In-depth	1579:1586	arg1	analysis					1596:1603	In-depth pathway analysis	1579:1603	In-depth pathway analysis	1579:1603	In-depth pathway analysis revealed that these metabolites are produced when cells utilize major energy sources such as glucose and select amino acids (tryptophan, arginine, isoleucine, and leucine) for growth, maintenance, and protein production.
34522610	5	32	theme	cell	729:732	arg1	metabolism					734:743	the inefficient cell metabolism	713:743	the inefficient cell metabolism	713:743	Six out of eight identified inhibitory metabolites, caused by the inefficient cell metabolism, were not previously studied in CHO cells: aconitic acid, 2-hydroxyisocaproic acid, methylsuccinic acid, cytidine monophosphate, trigonelline, and n-acetyl putrescine.
34522610	8	33	theme	protein	1806:1812	arg1	production					1814:1823	protein production	1806:1823	protein production	1806:1823	In-depth pathway analysis revealed that these metabolites are produced when cells utilize major energy sources such as glucose and select amino acids (tryptophan, arginine, isoleucine, and leucine) for growth, maintenance, and protein production.
34522610	1	34	theme	nutrients	163:171	arg1	amount					153:158	large amount	147:158	large amount of nutrients	147:171	Mammalian cells consume large amount of nutrients during growth and production.
34522610	1	34	theme	nutrients	163:171	arg1	nutrients					163:171	nutrients	163:171	nutrients	163:171	Mammalian cells consume large amount of nutrients during growth and production.
34522610	3	35	theme	nutrients	374:382	arg1	fraction					358:365	significant fraction	346:365	significant fraction of fed nutrients	346:382	Instead, significant fraction of fed nutrients is diverted into extracellular accumulation of waste by-products and metabolites, further inhibiting proliferation and protein synthesis.
34522610	0	36	from	Identification	0:13	arg1	growth					74:79	growth	74:79	growth	74:79	Identification of novel inhibitory metabolites and impact verification on growth and protein synthesis in mammalian cells.
34522610	0	36	from	Identification	0:13	arg1	synthesis					93:101	protein synthesis	85:101	protein synthesis	85:101	Identification of novel inhibitory metabolites and impact verification on growth and protein synthesis in mammalian cells.
34522610	6	37	dep	shown	1091:1095	arg1	secreted					1136:1143	secreted	1136:1143	secreted	1136:1143	When supplemented back into a fed-batch culture, significant reduction in cellular growth was observed in the presence of each metabolite and all the identified metabolites were shown to impact the glycosylation of a model secreted antibody, with seven of these also reducing CHO cellular productivity (titer) and all eight inhibiting the formation of mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans.
34522610	5	38	theme	CHO	777:779	arg1	cells					781:785	CHO cells	777:785	CHO cells	777:785	Six out of eight identified inhibitory metabolites, caused by the inefficient cell metabolism, were not previously studied in CHO cells: aconitic acid, 2-hydroxyisocaproic acid, methylsuccinic acid, cytidine monophosphate, trigonelline, and n-acetyl putrescine.
34522610	7	39	from	impact	1388:1393	arg1	culture					1517:1523	fed-batch culture	1507:1523	fed-batch culture	1507:1523	These inhibitory metabolites further impact the metabolism of cells, leading to a significant reduction in CHO cellular growth and specific productivity in fed-batch culture (maximum reductions of 27.2% and 40.6% respectively).
34522610	7	40	theme	inhibitory	1357:1366	arg1	metabolites					1368:1378	These inhibitory metabolites	1351:1378	These inhibitory metabolites	1351:1378	These inhibitory metabolites further impact the metabolism of cells, leading to a significant reduction in CHO cellular growth and specific productivity in fed-batch culture (maximum reductions of 27.2% and 40.6% respectively).
34522610	3	41	theme	extracellular	401:413	arg1	accumulation					415:426	extracellular accumulation	401:426	extracellular accumulation of waste by-products and metabolites	401:463	Instead, significant fraction of fed nutrients is diverted into extracellular accumulation of waste by-products and metabolites, further inhibiting proliferation and protein synthesis.
34522610	10	42	theme	biopharmaceutical	2271:2287	arg1	production					2289:2298	biopharmaceutical production	2271:2298	biopharmaceutical production	2271:2298	This study provides a robust and holistic methodology to incorporate global metabolomic analysis into cell culture studies for elucidation and structural verification of novel metabolites that participate in key metabolic pathways to growth, production, and post-translational modification in biopharmaceutical production.
34522610	3	43	theme	metabolites	453:463	arg1	accumulation					415:426	extracellular accumulation	401:426	extracellular accumulation of waste by-products and metabolites	401:463	Instead, significant fraction of fed nutrients is diverted into extracellular accumulation of waste by-products and metabolites, further inhibiting proliferation and protein synthesis.
34522610	8	44	dep	acids	1723:1727	arg1	tryptophan					1730:1739	tryptophan	1730:1739	tryptophan	1730:1739	In-depth pathway analysis revealed that these metabolites are produced when cells utilize major energy sources such as glucose and select amino acids (tryptophan, arginine, isoleucine, and leucine) for growth, maintenance, and protein production.
34522610	8	44	dep	acids	1723:1727	arg1	acids					1723:1727	select amino acids	1710:1727	select amino acids (tryptophan, arginine, isoleucine, and leucine)	1710:1775	In-depth pathway analysis revealed that these metabolites are produced when cells utilize major energy sources such as glucose and select amino acids (tryptophan, arginine, isoleucine, and leucine) for growth, maintenance, and protein production.
34522610	8	44	dep	acids	1723:1727	arg1	isoleucine					1752:1761	isoleucine	1752:1761	isoleucine	1752:1761	In-depth pathway analysis revealed that these metabolites are produced when cells utilize major energy sources such as glucose and select amino acids (tryptophan, arginine, isoleucine, and leucine) for growth, maintenance, and protein production.
34522610	8	44	dep	acids	1723:1727	arg1	leucine					1768:1774	leucine	1768:1774	leucine	1768:1774	In-depth pathway analysis revealed that these metabolites are produced when cells utilize major energy sources such as glucose and select amino acids (tryptophan, arginine, isoleucine, and leucine) for growth, maintenance, and protein production.
34522610	8	44	dep	acids	1723:1727	arg1	arginine					1742:1749	arginine	1742:1749	arginine	1742:1749	In-depth pathway analysis revealed that these metabolites are produced when cells utilize major energy sources such as glucose and select amino acids (tryptophan, arginine, isoleucine, and leucine) for growth, maintenance, and protein production.
34522610	3	45	theme	protein	503:509	arg1	synthesis					511:519	protein synthesis	503:519	protein synthesis	503:519	Instead, significant fraction of fed nutrients is diverted into extracellular accumulation of waste by-products and metabolites, further inhibiting proliferation and protein synthesis.
34522610	0	46	theme	novel	18:22	arg1	metabolites					35:45	novel inhibitory metabolites	18:45	novel inhibitory metabolites	18:45	Identification of novel inhibitory metabolites and impact verification on growth and protein synthesis in mammalian cells.
34522610	3	47	theme	by-products	437:447	arg1	accumulation					415:426	extracellular accumulation	401:426	extracellular accumulation of waste by-products and metabolites	401:463	Instead, significant fraction of fed nutrients is diverted into extracellular accumulation of waste by-products and metabolites, further inhibiting proliferation and protein synthesis.
34522610	4	48	theme	Chinese	596:602	arg1	CHO					619:621	CHO	619:621	CHO	619:621	In this study, an LC-MS/MS based metabolomics pipeline was used to screen Chinese hamster ovary (CHO) extracellular metabolites.
34522610	4	48	theme	Chinese	596:602	arg1	ovary					612:616	Chinese hamster ovary	596:616	Chinese hamster ovary (CHO) extracellular metabolites	596:648	In this study, an LC-MS/MS based metabolomics pipeline was used to screen Chinese hamster ovary (CHO) extracellular metabolites.
34522610	0	49	theme	metabolites	35:45	arg1	Identification					0:13	Identification	0:13	Identification of novel inhibitory metabolites and impact verification on growth and protein synthesis in mammalian cells.	0:121	Identification of novel inhibitory metabolites and impact verification on growth and protein synthesis in mammalian cells.
34522610	5	50	theme	2-hydroxyisocaproic	803:821	arg1	acid					823:826	2-hydroxyisocaproic acid	803:826	2-hydroxyisocaproic acid	803:826	Six out of eight identified inhibitory metabolites, caused by the inefficient cell metabolism, were not previously studied in CHO cells: aconitic acid, 2-hydroxyisocaproic acid, methylsuccinic acid, cytidine monophosphate, trigonelline, and n-acetyl putrescine.
34522610	7	51	theme	%	1552:1552	arg1	reductions					1534:1543	maximum reductions	1526:1543	maximum reductions of 27.2% and 40.6% respectively	1526:1575	These inhibitory metabolites further impact the metabolism of cells, leading to a significant reduction in CHO cellular growth and specific productivity in fed-batch culture (maximum reductions of 27.2% and 40.6% respectively).
34522610	6	52	theme	identified	1063:1072	arg1	metabolites					1074:1084	the identified metabolites	1059:1084	all the identified metabolites	1055:1084	When supplemented back into a fed-batch culture, significant reduction in cellular growth was observed in the presence of each metabolite and all the identified metabolites were shown to impact the glycosylation of a model secreted antibody, with seven of these also reducing CHO cellular productivity (titer) and all eight inhibiting the formation of mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans.
34522610	8	53	theme	pathway	1588:1594	arg1	analysis					1596:1603	In-depth pathway analysis	1579:1603	In-depth pathway analysis	1579:1603	In-depth pathway analysis revealed that these metabolites are produced when cells utilize major energy sources such as glucose and select amino acids (tryptophan, arginine, isoleucine, and leucine) for growth, maintenance, and protein production.
34522610	6	54	from	reduction	974:982	arg1	growth					996:1001	cellular growth	987:1001	cellular growth	987:1001	When supplemented back into a fed-batch culture, significant reduction in cellular growth was observed in the presence of each metabolite and all the identified metabolites were shown to impact the glycosylation of a model secreted antibody, with seven of these also reducing CHO cellular productivity (titer) and all eight inhibiting the formation of mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans.
34522610	0	55	theme	impact	51:56	arg1	verification					58:69	impact verification	51:69	impact verification	51:69	Identification of novel inhibitory metabolites and impact verification on growth and protein synthesis in mammalian cells.
34522610	4	56	used	used	581:584	arg2	pipeline					568:575	an LC-MS/MS based metabolomics pipeline	537:575	an LC-MS/MS based metabolomics pipeline	537:575	In this study, an LC-MS/MS based metabolomics pipeline was used to screen Chinese hamster ovary (CHO) extracellular metabolites.
34522610	9	57	theme	inhibitory	1851:1860	arg1	metabolites					1862:1872	these novel inhibitory metabolites	1839:1872	these novel inhibitory metabolites	1839:1872	Furthermore, these novel inhibitory metabolites were observed to accumulate in multiple CHO cell lines (CHO-K1 and CHO-GS) as well as HEK293 cell line.
34522610	6	58	located	observed	1007:1014	arg1	presence					1023:1030	the presence	1019:1030	the presence of each metabolite	1019:1049	When supplemented back into a fed-batch culture, significant reduction in cellular growth was observed in the presence of each metabolite and all the identified metabolites were shown to impact the glycosylation of a model secreted antibody, with seven of these also reducing CHO cellular productivity (titer) and all eight inhibiting the formation of mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans.
34522610	6	58	located	observed	1007:1014	arg2	reduction					974:982	significant reduction	962:982	significant reduction in cellular growth	962:1001	When supplemented back into a fed-batch culture, significant reduction in cellular growth was observed in the presence of each metabolite and all the identified metabolites were shown to impact the glycosylation of a model secreted antibody, with seven of these also reducing CHO cellular productivity (titer) and all eight inhibiting the formation of mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans.
34522610	8	59	theme	energy	1675:1680	arg1	acids					1723:1727	select amino acids	1710:1727	select amino acids (tryptophan, arginine, isoleucine, and leucine)	1710:1775	In-depth pathway analysis revealed that these metabolites are produced when cells utilize major energy sources such as glucose and select amino acids (tryptophan, arginine, isoleucine, and leucine) for growth, maintenance, and protein production.
34522610	8	59	theme	energy	1675:1680	arg1	sources					1682:1688	major energy sources	1669:1688	major energy sources such as glucose and select amino acids (tryptophan, arginine, isoleucine, and leucine) for growth, maintenance, and protein production	1669:1823	In-depth pathway analysis revealed that these metabolites are produced when cells utilize major energy sources such as glucose and select amino acids (tryptophan, arginine, isoleucine, and leucine) for growth, maintenance, and protein production.
34522610	8	59	theme	energy	1675:1680	arg1	glucose					1698:1704	glucose	1698:1704	glucose	1698:1704	In-depth pathway analysis revealed that these metabolites are produced when cells utilize major energy sources such as glucose and select amino acids (tryptophan, arginine, isoleucine, and leucine) for growth, maintenance, and protein production.
34522610	7	60	theme	maximum	1526:1532	arg1	reductions					1534:1543	maximum reductions	1526:1543	maximum reductions of 27.2% and 40.6% respectively	1526:1575	These inhibitory metabolites further impact the metabolism of cells, leading to a significant reduction in CHO cellular growth and specific productivity in fed-batch culture (maximum reductions of 27.2% and 40.6% respectively).
34522610	10	61	theme	metabolomic	2054:2064	arg1	analysis					2066:2073	global metabolomic analysis	2047:2073	global metabolomic analysis	2047:2073	This study provides a robust and holistic methodology to incorporate global metabolomic analysis into cell culture studies for elucidation and structural verification of novel metabolites that participate in key metabolic pathways to growth, production, and post-translational modification in biopharmaceutical production.
34522610	4	62	theme	extracellular	624:636	arg1	metabolites					638:648	Chinese hamster ovary (CHO) extracellular metabolites	596:648	Chinese hamster ovary (CHO) extracellular metabolites	596:648	In this study, an LC-MS/MS based metabolomics pipeline was used to screen Chinese hamster ovary (CHO) extracellular metabolites.
34522610	6	63	theme	model	1130:1134	arg1	glycosylation					1111:1123	the glycosylation	1107:1123	the glycosylation of a model	1107:1134	When supplemented back into a fed-batch culture, significant reduction in cellular growth was observed in the presence of each metabolite and all the identified metabolites were shown to impact the glycosylation of a model secreted antibody, with seven of these also reducing CHO cellular productivity (titer) and all eight inhibiting the formation of mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans.
34522610	4	64	theme	metabolomics	555:566	arg1	pipeline					568:575	an LC-MS/MS based metabolomics pipeline	537:575	an LC-MS/MS based metabolomics pipeline	537:575	In this study, an LC-MS/MS based metabolomics pipeline was used to screen Chinese hamster ovary (CHO) extracellular metabolites.
34522610	5	65	theme	aconitic	788:795	arg1	acid					797:800	aconitic acid	788:800	aconitic acid	788:800	Six out of eight identified inhibitory metabolites, caused by the inefficient cell metabolism, were not previously studied in CHO cells: aconitic acid, 2-hydroxyisocaproic acid, methylsuccinic acid, cytidine monophosphate, trigonelline, and n-acetyl putrescine.
34522610	5	66	theme	inhibitory	679:688	arg1	metabolites					690:700	eight identified inhibitory metabolites	662:700	eight identified inhibitory metabolites	662:700	Six out of eight identified inhibitory metabolites, caused by the inefficient cell metabolism, were not previously studied in CHO cells: aconitic acid, 2-hydroxyisocaproic acid, methylsuccinic acid, cytidine monophosphate, trigonelline, and n-acetyl putrescine.
34522610	6	67	theme	fed-batch	943:951	arg1	culture					953:959	a fed-batch culture	941:959	a fed-batch culture	941:959	When supplemented back into a fed-batch culture, significant reduction in cellular growth was observed in the presence of each metabolite and all the identified metabolites were shown to impact the glycosylation of a model secreted antibody, with seven of these also reducing CHO cellular productivity (titer) and all eight inhibiting the formation of mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans.
34522610	10	68	theme	culture	2085:2091	arg1	studies					2093:2099	cell culture studies	2080:2099	cell culture studies for elucidation and structural verification of novel metabolites that participate in key metabolic pathways to growth, production, and post-translational modification in biopharmaceutical production	2080:2298	This study provides a robust and holistic methodology to incorporate global metabolomic analysis into cell culture studies for elucidation and structural verification of novel metabolites that participate in key metabolic pathways to growth, production, and post-translational modification in biopharmaceutical production.
34522610	4	69	theme	ovary	612:616	arg1	metabolites					638:648	Chinese hamster ovary (CHO) extracellular metabolites	596:648	Chinese hamster ovary (CHO) extracellular metabolites	596:648	In this study, an LC-MS/MS based metabolomics pipeline was used to screen Chinese hamster ovary (CHO) extracellular metabolites.
34522610	8	70	theme	select	1710:1715	arg1	tryptophan					1730:1739	tryptophan	1730:1739	tryptophan	1730:1739	In-depth pathway analysis revealed that these metabolites are produced when cells utilize major energy sources such as glucose and select amino acids (tryptophan, arginine, isoleucine, and leucine) for growth, maintenance, and protein production.
34522610	8	70	theme	select	1710:1715	arg1	acids					1723:1727	select amino acids	1710:1727	select amino acids (tryptophan, arginine, isoleucine, and leucine)	1710:1775	In-depth pathway analysis revealed that these metabolites are produced when cells utilize major energy sources such as glucose and select amino acids (tryptophan, arginine, isoleucine, and leucine) for growth, maintenance, and protein production.
34522610	8	70	theme	select	1710:1715	arg1	isoleucine					1752:1761	isoleucine	1752:1761	isoleucine	1752:1761	In-depth pathway analysis revealed that these metabolites are produced when cells utilize major energy sources such as glucose and select amino acids (tryptophan, arginine, isoleucine, and leucine) for growth, maintenance, and protein production.
34522610	8	70	theme	select	1710:1715	arg1	leucine					1768:1774	leucine	1768:1774	leucine	1768:1774	In-depth pathway analysis revealed that these metabolites are produced when cells utilize major energy sources such as glucose and select amino acids (tryptophan, arginine, isoleucine, and leucine) for growth, maintenance, and protein production.
34522610	8	70	theme	select	1710:1715	arg1	arginine					1742:1749	arginine	1742:1749	arginine	1742:1749	In-depth pathway analysis revealed that these metabolites are produced when cells utilize major energy sources such as glucose and select amino acids (tryptophan, arginine, isoleucine, and leucine) for growth, maintenance, and protein production.
34522610	7	71	theme	specific	1482:1489	arg1	productivity					1491:1502	specific productivity	1482:1502	specific productivity	1482:1502	These inhibitory metabolites further impact the metabolism of cells, leading to a significant reduction in CHO cellular growth and specific productivity in fed-batch culture (maximum reductions of 27.2% and 40.6% respectively).
34522610	7	72	theme	cellular	1462:1469	arg1	growth					1471:1476	CHO cellular growth	1458:1476	CHO cellular growth	1458:1476	These inhibitory metabolites further impact the metabolism of cells, leading to a significant reduction in CHO cellular growth and specific productivity in fed-batch culture (maximum reductions of 27.2% and 40.6% respectively).
34522610	9	73	theme	CHO	1914:1916	arg1	lines					1923:1927	multiple CHO cell lines	1905:1927	multiple CHO cell lines (CHO-K1 and CHO-GS) as well as HEK293 cell line	1905:1975	Furthermore, these novel inhibitory metabolites were observed to accumulate in multiple CHO cell lines (CHO-K1 and CHO-GS) as well as HEK293 cell line.
34522610	9	73	theme	CHO	1914:1916	arg1	CHO-GS					1941:1946	CHO-GS	1941:1946	CHO-GS	1941:1946	Furthermore, these novel inhibitory metabolites were observed to accumulate in multiple CHO cell lines (CHO-K1 and CHO-GS) as well as HEK293 cell line.
34522610	9	73	theme	CHO	1914:1916	arg1	CHO-K1					1930:1935	CHO-K1	1930:1935	CHO-K1	1930:1935	Furthermore, these novel inhibitory metabolites were observed to accumulate in multiple CHO cell lines (CHO-K1 and CHO-GS) as well as HEK293 cell line.
34522610	9	74	theme	novel	1845:1849	arg1	metabolites					1862:1872	these novel inhibitory metabolites	1839:1872	these novel inhibitory metabolites	1839:1872	Furthermore, these novel inhibitory metabolites were observed to accumulate in multiple CHO cell lines (CHO-K1 and CHO-GS) as well as HEK293 cell line.
34522610	6	75	theme	CHO	1189:1191	arg1	titer					1216:1220	titer	1216:1220	titer	1216:1220	When supplemented back into a fed-batch culture, significant reduction in cellular growth was observed in the presence of each metabolite and all the identified metabolites were shown to impact the glycosylation of a model secreted antibody, with seven of these also reducing CHO cellular productivity (titer) and all eight inhibiting the formation of mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans.
34522610	6	75	theme	CHO	1189:1191	arg1	productivity					1202:1213	CHO cellular productivity	1189:1213	CHO cellular productivity (titer)	1189:1221	When supplemented back into a fed-batch culture, significant reduction in cellular growth was observed in the presence of each metabolite and all the identified metabolites were shown to impact the glycosylation of a model secreted antibody, with seven of these also reducing CHO cellular productivity (titer) and all eight inhibiting the formation of mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans.
34522610	6	76	theme	mono-galactosylated	1265:1283	arg1	G1F					1298:1300	mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans	1265:1348	G1F	1298:1300	When supplemented back into a fed-batch culture, significant reduction in cellular growth was observed in the presence of each metabolite and all the identified metabolites were shown to impact the glycosylation of a model secreted antibody, with seven of these also reducing CHO cellular productivity (titer) and all eight inhibiting the formation of mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans.
34522610	10	77	theme	cell	2080:2083	arg1	studies					2093:2099	cell culture studies	2080:2099	cell culture studies for elucidation and structural verification of novel metabolites that participate in key metabolic pathways to growth, production, and post-translational modification in biopharmaceutical production	2080:2298	This study provides a robust and holistic methodology to incorporate global metabolomic analysis into cell culture studies for elucidation and structural verification of novel metabolites that participate in key metabolic pathways to growth, production, and post-translational modification in biopharmaceutical production.
34522610	7	78	theme	significant	1433:1443	arg1	reduction					1445:1453	a significant reduction	1431:1453	a significant reduction in CHO cellular growth and specific productivity	1431:1502	These inhibitory metabolites further impact the metabolism of cells, leading to a significant reduction in CHO cellular growth and specific productivity in fed-batch culture (maximum reductions of 27.2% and 40.6% respectively).
34522610	2	79	theme	protein	317:323	arg1	production					325:334	protein production	317:334	protein production	317:334	However, endogenous metabolic inefficiencies often prevent cells to fully utilize nutrients to support growth and protein production.
34522610	10	80	theme	novel	2148:2152	arg1	metabolites					2154:2164	novel metabolites	2148:2164	novel metabolites that participate in key metabolic pathways to growth, production, and post-translational modification in biopharmaceutical production	2148:2298	This study provides a robust and holistic methodology to incorporate global metabolomic analysis into cell culture studies for elucidation and structural verification of novel metabolites that participate in key metabolic pathways to growth, production, and post-translational modification in biopharmaceutical production.
34522610	7	81	dep	impact	1388:1393	arg1	reductions					1534:1543	maximum reductions	1526:1543	maximum reductions of 27.2% and 40.6% respectively	1526:1575	These inhibitory metabolites further impact the metabolism of cells, leading to a significant reduction in CHO cellular growth and specific productivity in fed-batch culture (maximum reductions of 27.2% and 40.6% respectively).
34522610	6	82	theme	cellular	987:994	arg1	growth					996:1001	cellular growth	987:1001	cellular growth	987:1001	When supplemented back into a fed-batch culture, significant reduction in cellular growth was observed in the presence of each metabolite and all the identified metabolites were shown to impact the glycosylation of a model secreted antibody, with seven of these also reducing CHO cellular productivity (titer) and all eight inhibiting the formation of mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans.
34522610	0	83	from	growth	74:79	arg1	cells					116:120	mammalian cells	106:120	mammalian cells	106:120	Identification of novel inhibitory metabolites and impact verification on growth and protein synthesis in mammalian cells.
34522610	10	84	theme	metabolic	2190:2198	arg1	pathways					2200:2207	key metabolic pathways	2186:2207	key metabolic pathways to growth, production, and post-translational modification in biopharmaceutical production	2186:2298	This study provides a robust and holistic methodology to incorporate global metabolomic analysis into cell culture studies for elucidation and structural verification of novel metabolites that participate in key metabolic pathways to growth, production, and post-translational modification in biopharmaceutical production.
34522610	10	85	from	growth	2212:2217	arg1	production					2289:2298	biopharmaceutical production	2271:2298	biopharmaceutical production	2271:2298	This study provides a robust and holistic methodology to incorporate global metabolomic analysis into cell culture studies for elucidation and structural verification of novel metabolites that participate in key metabolic pathways to growth, production, and post-translational modification in biopharmaceutical production.
34522610	4	86	theme	LC-MS/MS	540:547	arg1	pipeline					568:575	an LC-MS/MS based metabolomics pipeline	537:575	an LC-MS/MS based metabolomics pipeline	537:575	In this study, an LC-MS/MS based metabolomics pipeline was used to screen Chinese hamster ovary (CHO) extracellular metabolites.
34522610	10	87	from	production	2220:2229	arg1	production					2289:2298	biopharmaceutical production	2271:2298	biopharmaceutical production	2271:2298	This study provides a robust and holistic methodology to incorporate global metabolomic analysis into cell culture studies for elucidation and structural verification of novel metabolites that participate in key metabolic pathways to growth, production, and post-translational modification in biopharmaceutical production.
34522610	5	88	theme	inefficient	717:727	arg1	metabolism					734:743	the inefficient cell metabolism	713:743	the inefficient cell metabolism	713:743	Six out of eight identified inhibitory metabolites, caused by the inefficient cell metabolism, were not previously studied in CHO cells: aconitic acid, 2-hydroxyisocaproic acid, methylsuccinic acid, cytidine monophosphate, trigonelline, and n-acetyl putrescine.
34522610	10	89	theme	robust	2000:2005	arg1	methodology					2020:2030	a robust and holistic methodology	1998:2030	a robust and holistic methodology to incorporate global metabolomic analysis into cell culture studies for elucidation and structural verification of novel metabolites that participate in key metabolic pathways to growth, production, and post-translational modification in biopharmaceutical production	1998:2298	This study provides a robust and holistic methodology to incorporate global metabolomic analysis into cell culture studies for elucidation and structural verification of novel metabolites that participate in key metabolic pathways to growth, production, and post-translational modification in biopharmaceutical production.
34522610	1	90	theme	large	147:151	arg1	amount					153:158	large amount	147:158	large amount of nutrients	147:171	Mammalian cells consume large amount of nutrients during growth and production.
34522610	1	90	theme	large	147:151	arg1	nutrients					163:171	nutrients	163:171	nutrients	163:171	Mammalian cells consume large amount of nutrients during growth and production.
34522610	10	91	from	modification	2255:2266	arg1	production					2289:2298	biopharmaceutical production	2271:2298	biopharmaceutical production	2271:2298	This study provides a robust and holistic methodology to incorporate global metabolomic analysis into cell culture studies for elucidation and structural verification of novel metabolites that participate in key metabolic pathways to growth, production, and post-translational modification in biopharmaceutical production.
34522610	0	92	theme	protein	85:91	arg1	synthesis					93:101	protein synthesis	85:101	protein synthesis	85:101	Identification of novel inhibitory metabolites and impact verification on growth and protein synthesis in mammalian cells.
34522610	4	93	theme	based	549:553	arg1	pipeline					568:575	an LC-MS/MS based metabolomics pipeline	537:575	an LC-MS/MS based metabolomics pipeline	537:575	In this study, an LC-MS/MS based metabolomics pipeline was used to screen Chinese hamster ovary (CHO) extracellular metabolites.
34522610	10	94	theme	post-translational	2236:2253	arg1	modification					2255:2266	post-translational modification	2236:2266	post-translational modification	2236:2266	This study provides a robust and holistic methodology to incorporate global metabolomic analysis into cell culture studies for elucidation and structural verification of novel metabolites that participate in key metabolic pathways to growth, production, and post-translational modification in biopharmaceutical production.
34522610	10	95	theme	holistic	2011:2018	arg1	methodology					2020:2030	a robust and holistic methodology	1998:2030	a robust and holistic methodology to incorporate global metabolomic analysis into cell culture studies for elucidation and structural verification of novel metabolites that participate in key metabolic pathways to growth, production, and post-translational modification in biopharmaceutical production	1998:2298	This study provides a robust and holistic methodology to incorporate global metabolomic analysis into cell culture studies for elucidation and structural verification of novel metabolites that participate in key metabolic pathways to growth, production, and post-translational modification in biopharmaceutical production.
34522610	3	96	theme	fed	370:372	arg1	nutrients					374:382	fed nutrients	370:382	fed nutrients	370:382	Instead, significant fraction of fed nutrients is diverted into extracellular accumulation of waste by-products and metabolites, further inhibiting proliferation and protein synthesis.
34522610	5	97	theme	methylsuccinic	829:842	arg1	acid					844:847	methylsuccinic acid	829:847	methylsuccinic acid	829:847	Six out of eight identified inhibitory metabolites, caused by the inefficient cell metabolism, were not previously studied in CHO cells: aconitic acid, 2-hydroxyisocaproic acid, methylsuccinic acid, cytidine monophosphate, trigonelline, and n-acetyl putrescine.
34522610	0	98	theme	inhibitory	24:33	arg1	metabolites					35:45	novel inhibitory metabolites	18:45	novel inhibitory metabolites	18:45	Identification of novel inhibitory metabolites and impact verification on growth and protein synthesis in mammalian cells.
34522610	5	99	dep	metabolites	690:700	arg1	out					655:657	out	655:657	out	655:657	Six out of eight identified inhibitory metabolites, caused by the inefficient cell metabolism, were not previously studied in CHO cells: aconitic acid, 2-hydroxyisocaproic acid, methylsuccinic acid, cytidine monophosphate, trigonelline, and n-acetyl putrescine.
34522610	4	100	theme	hamster	604:610	arg1	CHO					619:621	CHO	619:621	CHO	619:621	In this study, an LC-MS/MS based metabolomics pipeline was used to screen Chinese hamster ovary (CHO) extracellular metabolites.
34522610	4	100	theme	hamster	604:610	arg1	ovary					612:616	Chinese hamster ovary	596:616	Chinese hamster ovary (CHO) extracellular metabolites	596:648	In this study, an LC-MS/MS based metabolomics pipeline was used to screen Chinese hamster ovary (CHO) extracellular metabolites.
34522610	7	101	theme	%	1562:1562	arg1	reductions					1534:1543	maximum reductions	1526:1543	maximum reductions of 27.2% and 40.6% respectively	1526:1575	These inhibitory metabolites further impact the metabolism of cells, leading to a significant reduction in CHO cellular growth and specific productivity in fed-batch culture (maximum reductions of 27.2% and 40.6% respectively).
34522610	0	102	theme	mammalian	106:114	arg1	cells					116:120	mammalian cells	106:120	mammalian cells	106:120	Identification of novel inhibitory metabolites and impact verification on growth and protein synthesis in mammalian cells.
34522610	0	103	theme	verification	58:69	arg1	Identification					0:13	Identification	0:13	Identification of novel inhibitory metabolites and impact verification on growth and protein synthesis in mammalian cells.	0:121	Identification of novel inhibitory metabolites and impact verification on growth and protein synthesis in mammalian cells.
34522610	3	104	theme	waste	431:435	arg1	by-products					437:447	waste by-products	431:447	waste by-products	431:447	Instead, significant fraction of fed nutrients is diverted into extracellular accumulation of waste by-products and metabolites, further inhibiting proliferation and protein synthesis.
34522610	6	105	gly	glycosylation	1111:1123	arg1	model					1130:1134	a model	1128:1134	a model	1128:1134	When supplemented back into a fed-batch culture, significant reduction in cellular growth was observed in the presence of each metabolite and all the identified metabolites were shown to impact the glycosylation of a model secreted antibody, with seven of these also reducing CHO cellular productivity (titer) and all eight inhibiting the formation of mono-galactosylated biantennary (G1F) and biantennary galactosylated (G2F) N-glycans.
34522610	2	106	theme	endogenous	212:221	arg1	inefficiencies					233:246	endogenous metabolic inefficiencies	212:246	endogenous metabolic inefficiencies	212:246	However, endogenous metabolic inefficiencies often prevent cells to fully utilize nutrients to support growth and protein production.
34522610	10	107	theme	global	2047:2052	arg1	analysis					2066:2073	global metabolomic analysis	2047:2073	global metabolomic analysis	2047:2073	This study provides a robust and holistic methodology to incorporate global metabolomic analysis into cell culture studies for elucidation and structural verification of novel metabolites that participate in key metabolic pathways to growth, production, and post-translational modification in biopharmaceutical production.
32796603	5	0	dep	in	680:681	arg1	vivo					683:686	vivo	683:686	vivo	683:686	We used an in vivo rat model to study the scaffold effects when transplanted subcutaneously and as an augment for tendon repair.
32796603	10	1	dep	biocompatibility	1486:1501	arg1	only					1452:1455	only	1452:1455	only	1452:1455	This study demonstrated not only the collagen-alginate device biocompatibility, with no cytotoxic effects on the analyzed rats, but also that the 3D structure enables cell migration and new blood vessel formation needed for tissue repair.
32796603	5	2	theme	scaffold	711:718	arg1	effects					720:726	the scaffold effects	707:726	the scaffold effects when transplanted subcutaneously and as an augment for tendon repair	707:795	We used an in vivo rat model to study the scaffold effects when transplanted subcutaneously and as an augment for tendon repair.
32796603	1	3	theme	trauma	224:229	arg1	sites					231:235	trauma sites	224:235	trauma sites	224:235	Scaffold material is essential in providing mechanical support to tissue, allowing stem cells to improve their function in the healing and repair of trauma sites and tissue regeneration.
32796603	9	4	theme	transplanted	1260:1271	arg1	sites					1273:1277	the transplanted sites	1256:1277	the transplanted sites	1256:1277	At the transplanted sites, inflammatory cells, such as plasma cells, macrophages, and giant cells, were also observed as expected at this time point post transplantation.
32796603	3	5	from	site	493:496	arg1	differentiation					457:471	differentiation	457:471	differentiation	457:471	It serves and allows bio mimicking the mechanical and biological properties of the target tissue and facilitates cell proliferation and differentiation at the regeneration site.
32796603	3	5	from	site	493:496	arg1	proliferation					439:451	cell proliferation	434:451	cell proliferation	434:451	It serves and allows bio mimicking the mechanical and biological properties of the target tissue and facilitates cell proliferation and differentiation at the regeneration site.
32796603	11	6	theme	rats	1808:1811	arg1	well-being					1788:1797	the well-being	1784:1797	the well-being of these rats	1784:1811	Overall, the results of the current study proved for the first time that the implantable scaffold for long-term confirms the well-being of these rats and is correspondence to biocompatibility ISO standards and can be further developed for medical devices application.
32796603	1	7	theme	sites	231:235	arg1	repair					214:219	repair	214:219	repair	214:219	Scaffold material is essential in providing mechanical support to tissue, allowing stem cells to improve their function in the healing and repair of trauma sites and tissue regeneration.
32796603	1	7	theme	sites	231:235	arg1	healing					202:208	healing	202:208	healing	202:208	Scaffold material is essential in providing mechanical support to tissue, allowing stem cells to improve their function in the healing and repair of trauma sites and tissue regeneration.
32796603	1	7	theme	sites	231:235	arg1	regeneration					248:259	tissue regeneration	241:259	tissue regeneration	241:259	Scaffold material is essential in providing mechanical support to tissue, allowing stem cells to improve their function in the healing and repair of trauma sites and tissue regeneration.
32796603	10	8	theme	analyzed	1537:1544	arg1	rats					1546:1549	the analyzed rats	1533:1549	the analyzed rats	1533:1549	This study demonstrated not only the collagen-alginate device biocompatibility, with no cytotoxic effects on the analyzed rats, but also that the 3D structure enables cell migration and new blood vessel formation needed for tissue repair.
32796603	6	9	theme	scaffold	871:878	arg1	transplantation					880:894	scaffold transplantation	871:894	scaffold transplantation	871:894	Animals' well-being was measured by their weight and daily activity post scaffold transplantation during their recovery.
32796603	7	10	theme	scarring	1088:1095	arg1	tissue					1097:1102	scarring tissue	1088:1102	scarring tissue	1088:1102	At the end of the experiment, the bio-composite was histologically examined, and the surrounding tissues around the implant were evaluated for inflammation reaction and scarring tissue.
32796603	7	11	theme	experiment	937:946	arg1	end					926:928	the end	922:928	the end of the experiment	922:946	At the end of the experiment, the bio-composite was histologically examined, and the surrounding tissues around the implant were evaluated for inflammation reaction and scarring tissue.
32796603	2	12	theme	scaffold	266:273	arg1	organization					285:296	The scaffold aids cell organization	262:296	The scaffold aids cell organization in the damaged tissue	262:318	The scaffold aids cell organization in the damaged tissue.
32796603	2	13	from	organization	285:296	arg1	tissue					313:318	the damaged tissue	301:318	the damaged tissue	301:318	The scaffold aids cell organization in the damaged tissue.
32796603	11	14	theme	study	1699:1703	arg1	results					1676:1682	the results	1672:1682	the results of the current study	1672:1703	Overall, the results of the current study proved for the first time that the implantable scaffold for long-term confirms the well-being of these rats and is correspondence to biocompatibility ISO standards and can be further developed for medical devices application.
32796603	1	15	theme	tissue	241:246	arg1	regeneration					248:259	tissue regeneration	241:259	tissue regeneration	241:259	Scaffold material is essential in providing mechanical support to tissue, allowing stem cells to improve their function in the healing and repair of trauma sites and tissue regeneration.
32796603	8	16	theme	tissue	1152:1157	arg1	formation					1127:1135	the formation	1123:1135	the formation of granulation tissue and fibroblasts that were part of the inclusion process of the implanted material	1123:1239	In the histology, the formation of granulation tissue and fibroblasts that were part of the inclusion process of the implanted material were noted.
32796603	9	17	theme	inflammatory	1280:1291	arg1	macrophages					1322:1332	macrophages	1322:1332	macrophages	1322:1332	At the transplanted sites, inflammatory cells, such as plasma cells, macrophages, and giant cells, were also observed as expected at this time point post transplantation.
32796603	9	17	theme	inflammatory	1280:1291	arg1	cells					1315:1319	plasma cells	1308:1319	plasma cells	1308:1319	At the transplanted sites, inflammatory cells, such as plasma cells, macrophages, and giant cells, were also observed as expected at this time point post transplantation.
32796603	9	17	theme	inflammatory	1280:1291	arg1	cells					1293:1297	inflammatory cells	1280:1297	inflammatory cells	1280:1297	At the transplanted sites, inflammatory cells, such as plasma cells, macrophages, and giant cells, were also observed as expected at this time point post transplantation.
32796603	9	17	theme	inflammatory	1280:1291	arg1	cells					1345:1349	giant cells	1339:1349	giant cells	1339:1349	At the transplanted sites, inflammatory cells, such as plasma cells, macrophages, and giant cells, were also observed as expected at this time point post transplantation.
32796603	5	18	dep	an	768:769	arg1	augment					771:777	augment	771:777	augment for tendon repair	771:795	We used an in vivo rat model to study the scaffold effects when transplanted subcutaneously and as an augment for tendon repair.
32796603	10	19	theme	vessel	1620:1625	arg1	formation					1627:1635	new blood vessel formation	1610:1635	new blood vessel formation	1610:1635	This study demonstrated not only the collagen-alginate device biocompatibility, with no cytotoxic effects on the analyzed rats, but also that the 3D structure enables cell migration and new blood vessel formation needed for tissue repair.
32796603	11	20	theme	first	1720:1724	arg1	time					1726:1729	the first time that the implantable scaffold for long-term confirms the well-being of these rats and is correspondence to biocompatibility ISO standards and can be further developed for medical devices application	1716:1928	the first time that the implantable scaffold for long-term confirms the well-being of these rats and is correspondence to biocompatibility ISO standards and can be further developed for medical devices application	1716:1928	Overall, the results of the current study proved for the first time that the implantable scaffold for long-term confirms the well-being of these rats and is correspondence to biocompatibility ISO standards and can be further developed for medical devices application.
32796603	9	21	theme	time	1391:1394	arg1	point					1396:1400	this time point	1386:1400	this time point post transplantation	1386:1421	At the transplanted sites, inflammatory cells, such as plasma cells, macrophages, and giant cells, were also observed as expected at this time point post transplantation.
32796603	1	22	theme	stem	158:161	arg1	cells					163:167	stem cells	158:167	stem cells	158:167	Scaffold material is essential in providing mechanical support to tissue, allowing stem cells to improve their function in the healing and repair of trauma sites and tissue regeneration.
32796603	4	23	theme	collagen	569:576	arg1	fibers					578:583	unique collagen fibers	562:583	unique collagen fibers	562:583	In this study, the developed and assayed bio-composite made of unique collagen fibers and alginate hydrogel supports the function of cells around the implanted material.
32796603	6	24	theme	daily	851:855	arg1	activity					857:864	daily activity	851:864	daily activity	851:864	Animals' well-being was measured by their weight and daily activity post scaffold transplantation during their recovery.
32796603	4	25	theme	unique	562:567	arg1	fibers					578:583	unique collagen fibers	562:583	unique collagen fibers	562:583	In this study, the developed and assayed bio-composite made of unique collagen fibers and alginate hydrogel supports the function of cells around the implanted material.
32796603	9	26	located	observed	1362:1369	arg2	cells					1293:1297	inflammatory cells	1280:1297	inflammatory cells	1280:1297	At the transplanted sites, inflammatory cells, such as plasma cells, macrophages, and giant cells, were also observed as expected at this time point post transplantation.
32796603	9	26	located	observed	1362:1369	arg2	macrophages					1322:1332	macrophages	1322:1332	macrophages	1322:1332	At the transplanted sites, inflammatory cells, such as plasma cells, macrophages, and giant cells, were also observed as expected at this time point post transplantation.
32796603	9	26	located	observed	1362:1369	arg1	sites					1273:1277	the transplanted sites	1256:1277	the transplanted sites	1256:1277	At the transplanted sites, inflammatory cells, such as plasma cells, macrophages, and giant cells, were also observed as expected at this time point post transplantation.
32796603	9	26	located	observed	1362:1369	arg2	cells					1315:1319	plasma cells	1308:1319	plasma cells	1308:1319	At the transplanted sites, inflammatory cells, such as plasma cells, macrophages, and giant cells, were also observed as expected at this time point post transplantation.
32796603	9	26	located	observed	1362:1369	arg2	cells					1345:1349	giant cells	1339:1349	giant cells	1339:1349	At the transplanted sites, inflammatory cells, such as plasma cells, macrophages, and giant cells, were also observed as expected at this time point post transplantation.
32796603	5	27	used	used	672:675	arg2	We					669:670	We	669:670	We	669:670	We used an in vivo rat model to study the scaffold effects when transplanted subcutaneously and as an augment for tendon repair.
32796603	8	28	theme	process	1207:1213	arg1	part					1185:1188	part	1185:1188	part of the inclusion process of the implanted material	1185:1239	In the histology, the formation of granulation tissue and fibroblasts that were part of the inclusion process of the implanted material were noted.
32796603	8	29	theme	fibroblasts	1163:1173	arg1	formation					1127:1135	the formation	1123:1135	the formation of granulation tissue and fibroblasts that were part of the inclusion process of the implanted material	1123:1239	In the histology, the formation of granulation tissue and fibroblasts that were part of the inclusion process of the implanted material were noted.
32796603	11	30	theme	ISO	1855:1857	arg1	standards					1859:1867	biocompatibility ISO standards	1838:1867	biocompatibility ISO standards	1838:1867	Overall, the results of the current study proved for the first time that the implantable scaffold for long-term confirms the well-being of these rats and is correspondence to biocompatibility ISO standards and can be further developed for medical devices application.
32796603	1	31	theme	Scaffold	75:82	arg1	material					84:91	Scaffold material	75:91	Scaffold material	75:91	Scaffold material is essential in providing mechanical support to tissue, allowing stem cells to improve their function in the healing and repair of trauma sites and tissue regeneration.
32796603	8	32	theme	implanted	1222:1230	arg1	material					1232:1239	the implanted material	1218:1239	the implanted material	1218:1239	In the histology, the formation of granulation tissue and fibroblasts that were part of the inclusion process of the implanted material were noted.
32796603	3	33	theme	mechanical	360:369	arg1	properties					386:395	the mechanical and biological properties	356:395	the mechanical and biological properties of the target tissue	356:416	It serves and allows bio mimicking the mechanical and biological properties of the target tissue and facilitates cell proliferation and differentiation at the regeneration site.
32796603	4	34	theme	assayed	532:538	arg1	bio-composite					540:552	the developed and assayed bio-composite	514:552	the developed and assayed bio-composite made of unique collagen fibers and alginate hydrogel	514:605	In this study, the developed and assayed bio-composite made of unique collagen fibers and alginate hydrogel supports the function of cells around the implanted material.
32796603	7	35	theme	inflammation	1062:1073	arg1	reaction					1075:1082	inflammation reaction	1062:1082	inflammation reaction	1062:1082	At the end of the experiment, the bio-composite was histologically examined, and the surrounding tissues around the implant were evaluated for inflammation reaction and scarring tissue.
32796603	3	36	theme	target	404:409	arg1	tissue					411:416	the target tissue	400:416	the target tissue	400:416	It serves and allows bio mimicking the mechanical and biological properties of the target tissue and facilitates cell proliferation and differentiation at the regeneration site.
32796603	0	37	theme	Marine	22:27	arg1	Scaffold					44:51	a Marine Collagen-Based Scaffold	20:51	a Marine Collagen-Based Scaffold	20:51	Biocompatibility of a Marine Collagen-Based Scaffold In Vitro and In Vivo.
32796603	5	38	theme	tendon	783:788	arg1	repair					790:795	tendon repair	783:795	tendon repair	783:795	We used an in vivo rat model to study the scaffold effects when transplanted subcutaneously and as an augment for tendon repair.
32796603	5	39	theme	in	680:681	arg1	model					692:696	an in vivo rat model	677:696	an in vivo rat model	677:696	We used an in vivo rat model to study the scaffold effects when transplanted subcutaneously and as an augment for tendon repair.
32796603	10	40	theme	cell	1591:1594	arg1	migration					1596:1604	cell migration	1591:1604	cell migration	1591:1604	This study demonstrated not only the collagen-alginate device biocompatibility, with no cytotoxic effects on the analyzed rats, but also that the 3D structure enables cell migration and new blood vessel formation needed for tissue repair.
32796603	2	41	theme	cell	280:283	arg1	organization					285:296	The scaffold aids cell organization	262:296	The scaffold aids cell organization in the damaged tissue	262:318	The scaffold aids cell organization in the damaged tissue.
32796603	11	42	theme	current	1691:1697	arg1	study					1699:1703	the current study	1687:1703	the current study	1687:1703	Overall, the results of the current study proved for the first time that the implantable scaffold for long-term confirms the well-being of these rats and is correspondence to biocompatibility ISO standards and can be further developed for medical devices application.
32796603	9	43	theme	plasma	1308:1313	arg1	cells					1315:1319	plasma cells	1308:1319	plasma cells	1308:1319	At the transplanted sites, inflammatory cells, such as plasma cells, macrophages, and giant cells, were also observed as expected at this time point post transplantation.
32796603	2	44	theme	aids	275:278	arg1	organization					285:296	The scaffold aids cell organization	262:296	The scaffold aids cell organization in the damaged tissue	262:318	The scaffold aids cell organization in the damaged tissue.
32796603	8	45	theme	material	1232:1239	arg1	process					1207:1213	the inclusion process	1193:1213	the inclusion process of the implanted material	1193:1239	In the histology, the formation of granulation tissue and fibroblasts that were part of the inclusion process of the implanted material were noted.
32796603	0	46	theme	Scaffold	44:51	arg1	Biocompatibility					0:15	Biocompatibility	0:15	Biocompatibility of a Marine Collagen-Based Scaffold In Vitro and In Vivo.	0:73	Biocompatibility of a Marine Collagen-Based Scaffold In Vitro and In Vivo.
32796603	10	47	theme	tissue	1648:1653	arg1	repair					1655:1660	tissue repair	1648:1660	tissue repair	1648:1660	This study demonstrated not only the collagen-alginate device biocompatibility, with no cytotoxic effects on the analyzed rats, but also that the 3D structure enables cell migration and new blood vessel formation needed for tissue repair.
32796603	5	48	theme	rat	688:690	arg1	model					692:696	an in vivo rat model	677:696	an in vivo rat model	677:696	We used an in vivo rat model to study the scaffold effects when transplanted subcutaneously and as an augment for tendon repair.
32796603	10	49	theme	blood	1614:1618	arg1	formation					1627:1635	new blood vessel formation	1610:1635	new blood vessel formation	1610:1635	This study demonstrated not only the collagen-alginate device biocompatibility, with no cytotoxic effects on the analyzed rats, but also that the 3D structure enables cell migration and new blood vessel formation needed for tissue repair.
32796603	4	50	theme	implanted	649:657	arg1	material					659:666	the implanted material	645:666	the implanted material	645:666	In this study, the developed and assayed bio-composite made of unique collagen fibers and alginate hydrogel supports the function of cells around the implanted material.
32796603	4	51	theme	cells	632:636	arg1	function					620:627	the function	616:627	the function of cells around the implanted material	616:666	In this study, the developed and assayed bio-composite made of unique collagen fibers and alginate hydrogel supports the function of cells around the implanted material.
32796603	0	52	theme	Collagen-Based	29:42	arg1	Scaffold					44:51	a Marine Collagen-Based Scaffold	20:51	a Marine Collagen-Based Scaffold	20:51	Biocompatibility of a Marine Collagen-Based Scaffold In Vitro and In Vivo.
32796603	10	53	theme	3D	1570:1571	arg1	structure					1573:1581	the 3D structure	1566:1581	the 3D structure	1566:1581	This study demonstrated not only the collagen-alginate device biocompatibility, with no cytotoxic effects on the analyzed rats, but also that the 3D structure enables cell migration and new blood vessel formation needed for tissue repair.
32796603	1	54	dep	healing	202:208	arg1	the					198:200	the	198:200	the	198:200	Scaffold material is essential in providing mechanical support to tissue, allowing stem cells to improve their function in the healing and repair of trauma sites and tissue regeneration.
32796603	11	55	theme	medical	1902:1908	arg1	devices					1910:1916	medical devices	1902:1916	medical devices application	1902:1928	Overall, the results of the current study proved for the first time that the implantable scaffold for long-term confirms the well-being of these rats and is correspondence to biocompatibility ISO standards and can be further developed for medical devices application.
32796603	3	56	theme	cell	434:437	arg1	proliferation					439:451	cell proliferation	434:451	cell proliferation	434:451	It serves and allows bio mimicking the mechanical and biological properties of the target tissue and facilitates cell proliferation and differentiation at the regeneration site.
32796603	8	57	theme	inclusion	1197:1205	arg1	process					1207:1213	the inclusion process	1193:1213	the inclusion process of the implanted material	1193:1239	In the histology, the formation of granulation tissue and fibroblasts that were part of the inclusion process of the implanted material were noted.
32796603	10	58	dep	demonstrated	1435:1446	arg1	enables					1583:1589	enables	1583:1589	enables cell migration and new blood vessel formation needed for tissue repair	1583:1660	This study demonstrated not only the collagen-alginate device biocompatibility, with no cytotoxic effects on the analyzed rats, but also that the 3D structure enables cell migration and new blood vessel formation needed for tissue repair.
32796603	8	59	theme	granulation	1140:1150	arg1	tissue					1152:1157	granulation tissue	1140:1157	granulation tissue	1140:1157	In the histology, the formation of granulation tissue and fibroblasts that were part of the inclusion process of the implanted material were noted.
32796603	3	60	theme	tissue	411:416	arg1	properties					386:395	the mechanical and biological properties	356:395	the mechanical and biological properties of the target tissue	356:416	It serves and allows bio mimicking the mechanical and biological properties of the target tissue and facilitates cell proliferation and differentiation at the regeneration site.
32796603	3	61	theme	biological	375:384	arg1	properties					386:395	the mechanical and biological properties	356:395	the mechanical and biological properties of the target tissue	356:416	It serves and allows bio mimicking the mechanical and biological properties of the target tissue and facilitates cell proliferation and differentiation at the regeneration site.
32796603	4	62	theme	developed	518:526	arg1	bio-composite					540:552	the developed and assayed bio-composite	514:552	the developed and assayed bio-composite made of unique collagen fibers and alginate hydrogel	514:605	In this study, the developed and assayed bio-composite made of unique collagen fibers and alginate hydrogel supports the function of cells around the implanted material.
32796603	10	63	theme	device	1479:1484	arg1	biocompatibility					1486:1501	the collagen-alginate device biocompatibility	1457:1501	the collagen-alginate device biocompatibility	1457:1501	This study demonstrated not only the collagen-alginate device biocompatibility, with no cytotoxic effects on the analyzed rats, but also that the 3D structure enables cell migration and new blood vessel formation needed for tissue repair.
32796603	3	64	theme	regeneration	480:491	arg1	site					493:496	the regeneration site	476:496	the regeneration site	476:496	It serves and allows bio mimicking the mechanical and biological properties of the target tissue and facilitates cell proliferation and differentiation at the regeneration site.
32796603	10	65	theme	collagen-alginate	1461:1477	arg1	biocompatibility					1486:1501	the collagen-alginate device biocompatibility	1457:1501	the collagen-alginate device biocompatibility	1457:1501	This study demonstrated not only the collagen-alginate device biocompatibility, with no cytotoxic effects on the analyzed rats, but also that the 3D structure enables cell migration and new blood vessel formation needed for tissue repair.
32796603	10	66	from	effects	1522:1528	arg1	rats					1546:1549	the analyzed rats	1533:1549	the analyzed rats	1533:1549	This study demonstrated not only the collagen-alginate device biocompatibility, with no cytotoxic effects on the analyzed rats, but also that the 3D structure enables cell migration and new blood vessel formation needed for tissue repair.
32796603	1	67	from	function	186:193	arg1	repair					214:219	repair	214:219	repair	214:219	Scaffold material is essential in providing mechanical support to tissue, allowing stem cells to improve their function in the healing and repair of trauma sites and tissue regeneration.
32796603	1	67	from	function	186:193	arg1	healing					202:208	healing	202:208	healing	202:208	Scaffold material is essential in providing mechanical support to tissue, allowing stem cells to improve their function in the healing and repair of trauma sites and tissue regeneration.
32796603	1	67	from	function	186:193	arg1	regeneration					248:259	tissue regeneration	241:259	tissue regeneration	241:259	Scaffold material is essential in providing mechanical support to tissue, allowing stem cells to improve their function in the healing and repair of trauma sites and tissue regeneration.
32796603	1	68	theme	mechanical	119:128	arg1	support					130:136	mechanical support	119:136	mechanical support	119:136	Scaffold material is essential in providing mechanical support to tissue, allowing stem cells to improve their function in the healing and repair of trauma sites and tissue regeneration.
32796603	4	69	theme	alginate	589:596	arg1	hydrogel					598:605	alginate hydrogel	589:605	alginate hydrogel	589:605	In this study, the developed and assayed bio-composite made of unique collagen fibers and alginate hydrogel supports the function of cells around the implanted material.
32796603	7	70	theme	surrounding	1004:1014	arg1	tissues					1016:1022	the surrounding tissues	1000:1022	the surrounding tissues around the implant	1000:1041	At the end of the experiment, the bio-composite was histologically examined, and the surrounding tissues around the implant were evaluated for inflammation reaction and scarring tissue.
32796603	11	71	theme	implantable	1740:1750	arg1	scaffold					1752:1759	the implantable scaffold	1736:1759	the implantable scaffold for long-term	1736:1773	Overall, the results of the current study proved for the first time that the implantable scaffold for long-term confirms the well-being of these rats and is correspondence to biocompatibility ISO standards and can be further developed for medical devices application.
32796603	2	72	theme	damaged	305:311	arg1	tissue					313:318	the damaged tissue	301:318	the damaged tissue	301:318	The scaffold aids cell organization in the damaged tissue.
32796603	10	73	theme	cytotoxic	1512:1520	arg1	effects					1522:1528	no cytotoxic effects	1509:1528	no cytotoxic effects on the analyzed rats	1509:1549	This study demonstrated not only the collagen-alginate device biocompatibility, with no cytotoxic effects on the analyzed rats, but also that the 3D structure enables cell migration and new blood vessel formation needed for tissue repair.
32796603	10	74	theme	new	1610:1612	arg1	formation					1627:1635	new blood vessel formation	1610:1635	new blood vessel formation	1610:1635	This study demonstrated not only the collagen-alginate device biocompatibility, with no cytotoxic effects on the analyzed rats, but also that the 3D structure enables cell migration and new blood vessel formation needed for tissue repair.
32796603	11	75	theme	devices	1910:1916	arg1	application					1918:1928	medical devices application	1902:1928	medical devices application	1902:1928	Overall, the results of the current study proved for the first time that the implantable scaffold for long-term confirms the well-being of these rats and is correspondence to biocompatibility ISO standards and can be further developed for medical devices application.
32796603	11	76	theme	biocompatibility	1838:1853	arg1	standards					1859:1867	biocompatibility ISO standards	1838:1867	biocompatibility ISO standards	1838:1867	Overall, the results of the current study proved for the first time that the implantable scaffold for long-term confirms the well-being of these rats and is correspondence to biocompatibility ISO standards and can be further developed for medical devices application.
32796603	9	77	theme	giant	1339:1343	arg1	cells					1345:1349	giant cells	1339:1349	giant cells	1339:1349	At the transplanted sites, inflammatory cells, such as plasma cells, macrophages, and giant cells, were also observed as expected at this time point post transplantation.
32633312	0	0	theme	infected	104:111	arg1	wounds					113:118	infected wounds	104:118	infected wounds	104:118	A moisturizing chitosan-silk fibroin dressing with silver nanoparticles-adsorbed exosomes for repairing infected wounds.
32633312	4	1	theme	asymmetric	706:715	arg1	dressing					726:733	an asymmetric wettable dressing	703:733	an asymmetric wettable dressing with a composite of exosomes and silver nanoparticles (CTS-SF/SA/Ag-Exo dressing) for promoting angiogenesis, nerve repair and infected wound healing	703:883	In this study, we fabricated an asymmetric wettable dressing with a composite of exosomes and silver nanoparticles (CTS-SF/SA/Ag-Exo dressing) for promoting angiogenesis, nerve repair and infected wound healing.
32633312	0	2	with	dressing	37:44	arg1	silver					51:56	silver	51:56	silver	51:56	A moisturizing chitosan-silk fibroin dressing with silver nanoparticles-adsorbed exosomes for repairing infected wounds.
32633312	2	3	theme	strains	381:387	arg1	appearance					331:340	the appearance	327:340	the appearance of multidrug-resistant (MDR) bacterial strains, which pose a major challenge both in clinical and scientific research	327:458	In addition, large-area infected wounds cause the appearance of multidrug-resistant (MDR) bacterial strains, which pose a major challenge both in clinical and scientific research.
32633312	1	4	theme	appendages	269:278	arg1	repair					227:232	nerve system repair	214:232	nerve system repair	214:232	Refractory wounds caused by microbial infection impede wound healing, vascular regeneration, nerve system repair and the regeneration of other skin appendages.
32633312	1	4	theme	appendages	269:278	arg1	regeneration					242:253	the regeneration	238:253	the regeneration of other skin appendages	238:278	Refractory wounds caused by microbial infection impede wound healing, vascular regeneration, nerve system repair and the regeneration of other skin appendages.
32633312	1	4	theme	appendages	269:278	arg1	healing					182:188	wound healing	176:188	wound healing	176:188	Refractory wounds caused by microbial infection impede wound healing, vascular regeneration, nerve system repair and the regeneration of other skin appendages.
32633312	1	4	theme	appendages	269:278	arg1	regeneration					200:211	vascular regeneration	191:211	vascular regeneration	191:211	Refractory wounds caused by microbial infection impede wound healing, vascular regeneration, nerve system repair and the regeneration of other skin appendages.
32633312	7	5	theme	collagen	1304:1311	arg1	deposition					1313:1322	collagen deposition	1304:1322	collagen deposition	1304:1322	Moreover, the in vivo results show that the CTS-SF/SA/Ag-Exo dressing enhanced wound healing by accelerating collagen deposition, angiogenesis and nerve repair in a P. aeruginosa infected mouse skin wound defect model.
32633312	4	6	with	dressing	726:733	arg1	nanoparticles					775:787	exosomes and silver nanoparticles	755:787	exosomes and silver nanoparticles (CTS-SF/SA/Ag-Exo dressing)	755:815	In this study, we fabricated an asymmetric wettable dressing with a composite of exosomes and silver nanoparticles (CTS-SF/SA/Ag-Exo dressing) for promoting angiogenesis, nerve repair and infected wound healing.
32633312	4	6	with	dressing	726:733	arg1	composite					742:750	a composite	740:750	a composite of exosomes and silver nanoparticles (CTS-SF/SA/Ag-Exo dressing)	740:815	In this study, we fabricated an asymmetric wettable dressing with a composite of exosomes and silver nanoparticles (CTS-SF/SA/Ag-Exo dressing) for promoting angiogenesis, nerve repair and infected wound healing.
32633312	2	7	theme	bacterial	371:379	arg1	MDR					366:368	MDR	366:368	MDR	366:368	In addition, large-area infected wounds cause the appearance of multidrug-resistant (MDR) bacterial strains, which pose a major challenge both in clinical and scientific research.
32633312	2	7	theme	bacterial	371:379	arg1	strains					381:387	multidrug-resistant (MDR) bacterial strains	345:387	multidrug-resistant (MDR) bacterial strains	345:387	In addition, large-area infected wounds cause the appearance of multidrug-resistant (MDR) bacterial strains, which pose a major challenge both in clinical and scientific research.
32633312	6	8	theme	fibroblasts	1173:1183	arg1	proliferation					1150:1162	the proliferation	1146:1162	the proliferation of human fibroblasts	1146:1183	It can effectively inhibit the growth of bacterial and promote the proliferation of human fibroblasts in vitro.
32633312	8	9	theme	infected	1483:1490	arg1	wounds					1492:1497	infected wounds	1483:1497	infected wounds	1483:1497	We hope that this dressing will provide a solution for the repair of infected wounds for treatments in the clinic.
32633312	3	10	link	cell-derived	480:491	arg1	exosomes					493:500	many stem cell-derived exosomes	470:500	many stem cell-derived exosomes	470:500	Although many stem cell-derived exosomes have been demonstrated to promote skin repair and regeneration, exosomes are seldom applied in the treatment of infective wounds due to the lack of antimicrobial function.
32633312	2	11	dep	strains	381:387	arg1	both					419:422	both	419:422	both	419:422	In addition, large-area infected wounds cause the appearance of multidrug-resistant (MDR) bacterial strains, which pose a major challenge both in clinical and scientific research.
32633312	4	12	theme	exosomes	755:762	arg1	nanoparticles					775:787	exosomes and silver nanoparticles	755:787	exosomes and silver nanoparticles (CTS-SF/SA/Ag-Exo dressing)	755:815	In this study, we fabricated an asymmetric wettable dressing with a composite of exosomes and silver nanoparticles (CTS-SF/SA/Ag-Exo dressing) for promoting angiogenesis, nerve repair and infected wound healing.
32633312	4	12	theme	exosomes	755:762	arg1	dressing					807:814	CTS-SF/SA/Ag-Exo dressing	790:814	CTS-SF/SA/Ag-Exo dressing	790:814	In this study, we fabricated an asymmetric wettable dressing with a composite of exosomes and silver nanoparticles (CTS-SF/SA/Ag-Exo dressing) for promoting angiogenesis, nerve repair and infected wound healing.
32633312	4	13	theme	silver	768:773	arg1	nanoparticles					775:787	exosomes and silver nanoparticles	755:787	exosomes and silver nanoparticles (CTS-SF/SA/Ag-Exo dressing)	755:815	In this study, we fabricated an asymmetric wettable dressing with a composite of exosomes and silver nanoparticles (CTS-SF/SA/Ag-Exo dressing) for promoting angiogenesis, nerve repair and infected wound healing.
32633312	4	13	theme	silver	768:773	arg1	dressing					807:814	CTS-SF/SA/Ag-Exo dressing	790:814	CTS-SF/SA/Ag-Exo dressing	790:814	In this study, we fabricated an asymmetric wettable dressing with a composite of exosomes and silver nanoparticles (CTS-SF/SA/Ag-Exo dressing) for promoting angiogenesis, nerve repair and infected wound healing.
32633312	2	14	theme	infected	305:312	arg1	wounds					314:319	large-area infected wounds	294:319	large-area infected wounds	294:319	In addition, large-area infected wounds cause the appearance of multidrug-resistant (MDR) bacterial strains, which pose a major challenge both in clinical and scientific research.
32633312	7	15	theme	P.	1360:1361	arg1	model					1407:1411	a P. aeruginosa infected mouse skin wound defect model	1358:1411	a P. aeruginosa infected mouse skin wound defect model	1358:1411	Moreover, the in vivo results show that the CTS-SF/SA/Ag-Exo dressing enhanced wound healing by accelerating collagen deposition, angiogenesis and nerve repair in a P. aeruginosa infected mouse skin wound defect model.
32633312	4	16	theme	infected	862:869	arg1	healing					877:883	infected wound healing	862:883	infected wound healing	862:883	In this study, we fabricated an asymmetric wettable dressing with a composite of exosomes and silver nanoparticles (CTS-SF/SA/Ag-Exo dressing) for promoting angiogenesis, nerve repair and infected wound healing.
32633312	7	17	theme	wound	1394:1398	arg1	model					1407:1411	a P. aeruginosa infected mouse skin wound defect model	1358:1411	a P. aeruginosa infected mouse skin wound defect model	1358:1411	Moreover, the in vivo results show that the CTS-SF/SA/Ag-Exo dressing enhanced wound healing by accelerating collagen deposition, angiogenesis and nerve repair in a P. aeruginosa infected mouse skin wound defect model.
32633312	2	18	theme	large-area	294:303	arg1	wounds					314:319	large-area infected wounds	294:319	large-area infected wounds	294:319	In addition, large-area infected wounds cause the appearance of multidrug-resistant (MDR) bacterial strains, which pose a major challenge both in clinical and scientific research.
32633312	7	19	theme	defect	1400:1405	arg1	model					1407:1411	a P. aeruginosa infected mouse skin wound defect model	1358:1411	a P. aeruginosa infected mouse skin wound defect model	1358:1411	Moreover, the in vivo results show that the CTS-SF/SA/Ag-Exo dressing enhanced wound healing by accelerating collagen deposition, angiogenesis and nerve repair in a P. aeruginosa infected mouse skin wound defect model.
32633312	1	20	theme	vascular	191:198	arg1	regeneration					200:211	vascular regeneration	191:211	vascular regeneration	191:211	Refractory wounds caused by microbial infection impede wound healing, vascular regeneration, nerve system repair and the regeneration of other skin appendages.
32633312	7	21	theme	wound	1274:1278	arg1	healing					1280:1286	wound healing	1274:1286	wound healing	1274:1286	Moreover, the in vivo results show that the CTS-SF/SA/Ag-Exo dressing enhanced wound healing by accelerating collagen deposition, angiogenesis and nerve repair in a P. aeruginosa infected mouse skin wound defect model.
32633312	6	22	theme	human	1167:1171	arg1	fibroblasts					1173:1183	human fibroblasts	1167:1183	human fibroblasts	1167:1183	It can effectively inhibit the growth of bacterial and promote the proliferation of human fibroblasts in vitro.
32633312	2	23	theme	major	403:407	arg1	challenge					409:417	a major challenge	401:417	a major challenge	401:417	In addition, large-area infected wounds cause the appearance of multidrug-resistant (MDR) bacterial strains, which pose a major challenge both in clinical and scientific research.
32633312	0	24	theme	moisturizing	2:13	arg1	dressing					37:44	A moisturizing chitosan-silk fibroin dressing	0:44	A moisturizing chitosan-silk fibroin dressing with silver	0:56	A moisturizing chitosan-silk fibroin dressing with silver nanoparticles-adsorbed exosomes for repairing infected wounds.
32633312	7	25	theme	infected	1374:1381	arg1	model					1407:1411	a P. aeruginosa infected mouse skin wound defect model	1358:1411	a P. aeruginosa infected mouse skin wound defect model	1358:1411	Moreover, the in vivo results show that the CTS-SF/SA/Ag-Exo dressing enhanced wound healing by accelerating collagen deposition, angiogenesis and nerve repair in a P. aeruginosa infected mouse skin wound defect model.
32633312	7	26	theme	CTS-SF/SA/Ag-Exo	1239:1254	arg1	dressing					1256:1263	the CTS-SF/SA/Ag-Exo dressing	1235:1263	the CTS-SF/SA/Ag-Exo dressing	1235:1263	Moreover, the in vivo results show that the CTS-SF/SA/Ag-Exo dressing enhanced wound healing by accelerating collagen deposition, angiogenesis and nerve repair in a P. aeruginosa infected mouse skin wound defect model.
32633312	5	27	theme	CTS-SF/SA/Ag-Exo	890:905	arg1	dressing					907:914	The CTS-SF/SA/Ag-Exo dressing	886:914	The CTS-SF/SA/Ag-Exo dressing	886:914	The CTS-SF/SA/Ag-Exo dressing possesses multifunctional properties including broad-spectrum antimicrobial activity, promoting wound healing, retaining moisture and maintaining electrolyte balance.
32633312	4	28	theme	wound	871:875	arg1	healing					877:883	infected wound healing	862:883	infected wound healing	862:883	In this study, we fabricated an asymmetric wettable dressing with a composite of exosomes and silver nanoparticles (CTS-SF/SA/Ag-Exo dressing) for promoting angiogenesis, nerve repair and infected wound healing.
32633312	5	29	theme	wound	1012:1016	arg1	healing					1018:1024	wound healing	1012:1024	wound healing	1012:1024	The CTS-SF/SA/Ag-Exo dressing possesses multifunctional properties including broad-spectrum antimicrobial activity, promoting wound healing, retaining moisture and maintaining electrolyte balance.
32633312	8	30	from	treatments	1503:1512	arg1	clinic					1521:1526	the clinic	1517:1526	the clinic	1517:1526	We hope that this dressing will provide a solution for the repair of infected wounds for treatments in the clinic.
32633312	1	31	theme	nerve	214:218	arg1	repair					227:232	nerve system repair	214:232	nerve system repair	214:232	Refractory wounds caused by microbial infection impede wound healing, vascular regeneration, nerve system repair and the regeneration of other skin appendages.
32633312	0	32	theme	fibroin	29:35	arg1	dressing					37:44	A moisturizing chitosan-silk fibroin dressing	0:44	A moisturizing chitosan-silk fibroin dressing with silver	0:56	A moisturizing chitosan-silk fibroin dressing with silver nanoparticles-adsorbed exosomes for repairing infected wounds.
32633312	1	33	theme	Refractory	121:130	arg1	wounds					132:137	Refractory wounds	121:137	Refractory wounds caused by microbial infection	121:167	Refractory wounds caused by microbial infection impede wound healing, vascular regeneration, nerve system repair and the regeneration of other skin appendages.
32633312	1	34	theme	system	220:225	arg1	repair					227:232	nerve system repair	214:232	nerve system repair	214:232	Refractory wounds caused by microbial infection impede wound healing, vascular regeneration, nerve system repair and the regeneration of other skin appendages.
32633312	2	35	theme	scientific	440:449	arg1	research					451:458	scientific research	440:458	scientific research	440:458	In addition, large-area infected wounds cause the appearance of multidrug-resistant (MDR) bacterial strains, which pose a major challenge both in clinical and scientific research.
32633312	7	36	dep	P.	1360:1361	arg1	aeruginosa					1363:1372	aeruginosa	1363:1372	aeruginosa	1363:1372	Moreover, the in vivo results show that the CTS-SF/SA/Ag-Exo dressing enhanced wound healing by accelerating collagen deposition, angiogenesis and nerve repair in a P. aeruginosa infected mouse skin wound defect model.
32633312	3	37	theme	function	664:671	arg1	lack					642:645	the lack	638:645	the lack of antimicrobial function	638:671	Although many stem cell-derived exosomes have been demonstrated to promote skin repair and regeneration, exosomes are seldom applied in the treatment of infective wounds due to the lack of antimicrobial function.
32633312	3	38	theme	skin	536:539	arg1	repair					541:546	skin repair	536:546	skin repair	536:546	Although many stem cell-derived exosomes have been demonstrated to promote skin repair and regeneration, exosomes are seldom applied in the treatment of infective wounds due to the lack of antimicrobial function.
32633312	6	39	theme	bacterial	1124:1132	arg1	growth					1114:1119	the growth	1110:1119	the growth of bacterial	1110:1132	It can effectively inhibit the growth of bacterial and promote the proliferation of human fibroblasts in vitro.
32633312	5	40	theme	antimicrobial	978:990	arg1	activity					992:999	broad-spectrum antimicrobial activity	963:999	broad-spectrum antimicrobial activity	963:999	The CTS-SF/SA/Ag-Exo dressing possesses multifunctional properties including broad-spectrum antimicrobial activity, promoting wound healing, retaining moisture and maintaining electrolyte balance.
32633312	7	41	theme	nerve	1342:1346	arg1	repair					1348:1353	nerve repair	1342:1353	nerve repair	1342:1353	Moreover, the in vivo results show that the CTS-SF/SA/Ag-Exo dressing enhanced wound healing by accelerating collagen deposition, angiogenesis and nerve repair in a P. aeruginosa infected mouse skin wound defect model.
32633312	3	42	theme	infective	614:622	arg1	wounds					624:629	infective wounds	614:629	infective wounds due to the lack of antimicrobial function	614:671	Although many stem cell-derived exosomes have been demonstrated to promote skin repair and regeneration, exosomes are seldom applied in the treatment of infective wounds due to the lack of antimicrobial function.
32633312	3	43	theme	many	470:473	arg1	exosomes					493:500	many stem cell-derived exosomes	470:500	many stem cell-derived exosomes	470:500	Although many stem cell-derived exosomes have been demonstrated to promote skin repair and regeneration, exosomes are seldom applied in the treatment of infective wounds due to the lack of antimicrobial function.
32633312	3	44	theme	wounds	624:629	arg1	treatment					601:609	the treatment	597:609	the treatment of infective wounds due to the lack of antimicrobial function	597:671	Although many stem cell-derived exosomes have been demonstrated to promote skin repair and regeneration, exosomes are seldom applied in the treatment of infective wounds due to the lack of antimicrobial function.
32633312	3	45	theme	stem	475:478	arg1	exosomes					493:500	many stem cell-derived exosomes	470:500	many stem cell-derived exosomes	470:500	Although many stem cell-derived exosomes have been demonstrated to promote skin repair and regeneration, exosomes are seldom applied in the treatment of infective wounds due to the lack of antimicrobial function.
32633312	5	46	contain	possesses	916:924	arg2	properties					942:951	multifunctional properties	926:951	multifunctional properties including broad-spectrum antimicrobial activity	926:999	The CTS-SF/SA/Ag-Exo dressing possesses multifunctional properties including broad-spectrum antimicrobial activity, promoting wound healing, retaining moisture and maintaining electrolyte balance.
32633312	5	46	contain	possesses	916:924	arg2	activity					992:999	broad-spectrum antimicrobial activity	963:999	broad-spectrum antimicrobial activity	963:999	The CTS-SF/SA/Ag-Exo dressing possesses multifunctional properties including broad-spectrum antimicrobial activity, promoting wound healing, retaining moisture and maintaining electrolyte balance.
32633312	5	46	contain	possesses	916:924	arg1	dressing					907:914	The CTS-SF/SA/Ag-Exo dressing	886:914	The CTS-SF/SA/Ag-Exo dressing	886:914	The CTS-SF/SA/Ag-Exo dressing possesses multifunctional properties including broad-spectrum antimicrobial activity, promoting wound healing, retaining moisture and maintaining electrolyte balance.
32633312	3	47	theme	antimicrobial	650:662	arg1	function					664:671	antimicrobial function	650:671	antimicrobial function	650:671	Although many stem cell-derived exosomes have been demonstrated to promote skin repair and regeneration, exosomes are seldom applied in the treatment of infective wounds due to the lack of antimicrobial function.
32633312	5	48	theme	broad-spectrum	963:976	arg1	activity					992:999	broad-spectrum antimicrobial activity	963:999	broad-spectrum antimicrobial activity	963:999	The CTS-SF/SA/Ag-Exo dressing possesses multifunctional properties including broad-spectrum antimicrobial activity, promoting wound healing, retaining moisture and maintaining electrolyte balance.
32633312	4	49	theme	nerve	845:849	arg1	repair					851:856	nerve repair	845:856	nerve repair	845:856	In this study, we fabricated an asymmetric wettable dressing with a composite of exosomes and silver nanoparticles (CTS-SF/SA/Ag-Exo dressing) for promoting angiogenesis, nerve repair and infected wound healing.
32633312	4	50	theme	wettable	717:724	arg1	dressing					726:733	an asymmetric wettable dressing	703:733	an asymmetric wettable dressing with a composite of exosomes and silver nanoparticles (CTS-SF/SA/Ag-Exo dressing) for promoting angiogenesis, nerve repair and infected wound healing	703:883	In this study, we fabricated an asymmetric wettable dressing with a composite of exosomes and silver nanoparticles (CTS-SF/SA/Ag-Exo dressing) for promoting angiogenesis, nerve repair and infected wound healing.
32633312	8	51	theme	wounds	1492:1497	arg1	repair					1473:1478	the repair	1469:1478	the repair of infected wounds for treatments in the clinic	1469:1526	We hope that this dressing will provide a solution for the repair of infected wounds for treatments in the clinic.
32633312	5	52	theme	electrolyte	1062:1072	arg1	balance					1074:1080	electrolyte balance	1062:1080	electrolyte balance	1062:1080	The CTS-SF/SA/Ag-Exo dressing possesses multifunctional properties including broad-spectrum antimicrobial activity, promoting wound healing, retaining moisture and maintaining electrolyte balance.
32633312	1	53	theme	microbial	149:157	arg1	infection					159:167	microbial infection	149:167	microbial infection	149:167	Refractory wounds caused by microbial infection impede wound healing, vascular regeneration, nerve system repair and the regeneration of other skin appendages.
32633312	5	54	dep	possesses	916:924	arg1	promoting					1002:1010	promoting	1002:1010	promoting wound healing	1002:1024	The CTS-SF/SA/Ag-Exo dressing possesses multifunctional properties including broad-spectrum antimicrobial activity, promoting wound healing, retaining moisture and maintaining electrolyte balance.
32633312	5	54	dep	possesses	916:924	arg1	retaining					1027:1035	retaining	1027:1035	retaining moisture	1027:1044	The CTS-SF/SA/Ag-Exo dressing possesses multifunctional properties including broad-spectrum antimicrobial activity, promoting wound healing, retaining moisture and maintaining electrolyte balance.
32633312	5	54	dep	possesses	916:924	arg1	maintaining					1050:1060	maintaining	1050:1060	maintaining electrolyte balance	1050:1080	The CTS-SF/SA/Ag-Exo dressing possesses multifunctional properties including broad-spectrum antimicrobial activity, promoting wound healing, retaining moisture and maintaining electrolyte balance.
32633312	4	55	theme	nanoparticles	775:787	arg1	nanoparticles					775:787	exosomes and silver nanoparticles	755:787	exosomes and silver nanoparticles (CTS-SF/SA/Ag-Exo dressing)	755:815	In this study, we fabricated an asymmetric wettable dressing with a composite of exosomes and silver nanoparticles (CTS-SF/SA/Ag-Exo dressing) for promoting angiogenesis, nerve repair and infected wound healing.
32633312	4	55	theme	nanoparticles	775:787	arg1	composite					742:750	a composite	740:750	a composite of exosomes and silver nanoparticles (CTS-SF/SA/Ag-Exo dressing)	740:815	In this study, we fabricated an asymmetric wettable dressing with a composite of exosomes and silver nanoparticles (CTS-SF/SA/Ag-Exo dressing) for promoting angiogenesis, nerve repair and infected wound healing.
32633312	2	56	theme	multidrug-resistant	345:363	arg1	MDR					366:368	MDR	366:368	MDR	366:368	In addition, large-area infected wounds cause the appearance of multidrug-resistant (MDR) bacterial strains, which pose a major challenge both in clinical and scientific research.
32633312	2	56	theme	multidrug-resistant	345:363	arg1	strains					381:387	multidrug-resistant (MDR) bacterial strains	345:387	multidrug-resistant (MDR) bacterial strains	345:387	In addition, large-area infected wounds cause the appearance of multidrug-resistant (MDR) bacterial strains, which pose a major challenge both in clinical and scientific research.
32633312	7	57	theme	mouse	1383:1387	arg1	model					1407:1411	a P. aeruginosa infected mouse skin wound defect model	1358:1411	a P. aeruginosa infected mouse skin wound defect model	1358:1411	Moreover, the in vivo results show that the CTS-SF/SA/Ag-Exo dressing enhanced wound healing by accelerating collagen deposition, angiogenesis and nerve repair in a P. aeruginosa infected mouse skin wound defect model.
32633312	3	58	theme	due	631:633	arg1	wounds					624:629	infective wounds	614:629	infective wounds due to the lack of antimicrobial function	614:671	Although many stem cell-derived exosomes have been demonstrated to promote skin repair and regeneration, exosomes are seldom applied in the treatment of infective wounds due to the lack of antimicrobial function.
32633312	3	59	theme	cell-derived	480:491	arg1	exosomes					493:500	many stem cell-derived exosomes	470:500	many stem cell-derived exosomes	470:500	Although many stem cell-derived exosomes have been demonstrated to promote skin repair and regeneration, exosomes are seldom applied in the treatment of infective wounds due to the lack of antimicrobial function.
32633312	5	60	theme	multifunctional	926:940	arg1	properties					942:951	multifunctional properties	926:951	multifunctional properties including broad-spectrum antimicrobial activity	926:999	The CTS-SF/SA/Ag-Exo dressing possesses multifunctional properties including broad-spectrum antimicrobial activity, promoting wound healing, retaining moisture and maintaining electrolyte balance.
32633312	5	60	theme	multifunctional	926:940	arg1	activity					992:999	broad-spectrum antimicrobial activity	963:999	broad-spectrum antimicrobial activity	963:999	The CTS-SF/SA/Ag-Exo dressing possesses multifunctional properties including broad-spectrum antimicrobial activity, promoting wound healing, retaining moisture and maintaining electrolyte balance.
32633312	7	61	theme	skin	1389:1392	arg1	model					1407:1411	a P. aeruginosa infected mouse skin wound defect model	1358:1411	a P. aeruginosa infected mouse skin wound defect model	1358:1411	Moreover, the in vivo results show that the CTS-SF/SA/Ag-Exo dressing enhanced wound healing by accelerating collagen deposition, angiogenesis and nerve repair in a P. aeruginosa infected mouse skin wound defect model.
32633312	1	62	theme	other	258:262	arg1	appendages					269:278	other skin appendages	258:278	other skin appendages	258:278	Refractory wounds caused by microbial infection impede wound healing, vascular regeneration, nerve system repair and the regeneration of other skin appendages.
32633312	7	63	theme	in	1209:1210	arg1	results					1217:1223	the in vivo results	1205:1223	the in vivo results	1205:1223	Moreover, the in vivo results show that the CTS-SF/SA/Ag-Exo dressing enhanced wound healing by accelerating collagen deposition, angiogenesis and nerve repair in a P. aeruginosa infected mouse skin wound defect model.
32633312	0	64	theme	chitosan-silk	15:27	arg1	dressing					37:44	A moisturizing chitosan-silk fibroin dressing	0:44	A moisturizing chitosan-silk fibroin dressing with silver	0:56	A moisturizing chitosan-silk fibroin dressing with silver nanoparticles-adsorbed exosomes for repairing infected wounds.
32633312	1	65	theme	wound	176:180	arg1	healing					182:188	wound healing	176:188	wound healing	176:188	Refractory wounds caused by microbial infection impede wound healing, vascular regeneration, nerve system repair and the regeneration of other skin appendages.
32633312	7	66	dep	in	1209:1210	arg1	vivo					1212:1215	vivo	1212:1215	vivo	1212:1215	Moreover, the in vivo results show that the CTS-SF/SA/Ag-Exo dressing enhanced wound healing by accelerating collagen deposition, angiogenesis and nerve repair in a P. aeruginosa infected mouse skin wound defect model.
32633312	1	67	theme	skin	264:267	arg1	appendages					269:278	other skin appendages	258:278	other skin appendages	258:278	Refractory wounds caused by microbial infection impede wound healing, vascular regeneration, nerve system repair and the regeneration of other skin appendages.
32065574	3	0	dep	Tamarin	278:284	arg1	chrysomelas					302:312	Leontopithecus chrysomelas	287:312	Leontopithecus chrysomelas	287:312	Two Bifidobacterium strains, i.e., 2176BT and 2177BT, were isolated from Golden-Headed Lion Tamarin (Leontopithecus chrysomelas) and Goeldi's monkey (Callimico goeldii).
32065574	5	1	theme	Bifidobacterium	784:798	arg1	11041T					812:817	Bifidobacterium bifidum LMG 11041T	784:817	Bifidobacterium bifidum LMG 11041T	784:817	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	9	2	theme	2177BT=LMG	1183:1192	arg1	73786T					1206:1211	2177BT=LMG 31471T=CCUG 73786T	1183:1211	nov. (2177BT=LMG 31471T=CCUG 73786T	1177:1211	nov. (2177BT=LMG 31471T=CCUG 73786T are proposed as novel Bifidobacterium species.
32065574	5	3	theme	close	708:712	arg1	relatedness					727:737	close phylogenetic relatedness	708:737	close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T	708:817	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	9	4	theme	31471T=CCUG	1194:1204	arg1	73786T					1206:1211	2177BT=LMG 31471T=CCUG 73786T	1183:1211	nov. (2177BT=LMG 31471T=CCUG 73786T	1177:1211	nov. (2177BT=LMG 31471T=CCUG 73786T are proposed as novel Bifidobacterium species.
32065574	6	5	theme	Further	834:840	arg1	genotyping					842:851	Further genotyping	834:851	Further genotyping	834:851	Further genotyping based on the genome sequence of the isolated strains combined with phenotypic analyses, clearly show that these strains are distinct from each of the type strains of the so far recognized Bifidobacterium species.
32065574	0	6	theme	genus	85:89	arg1	Bifidobacterium					91:105	the genus Bifidobacterium	81:105	the genus Bifidobacterium	81:105	Characterization of the phylogenetic diversity of two novel species belonging to the genus Bifidobacterium: Bifidobacterium cebidarum sp.
32065574	5	7	theme	bifidobacterial	658:672	arg1	strains					674:680	bifidobacterial strains	658:680	bifidobacterial strains 2176BT and 2177BT	658:698	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	5	7	theme	bifidobacterial	658:672	arg1	2177BT					693:698	2177BT	693:698	2177BT	693:698	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	5	7	theme	bifidobacterial	658:672	arg1	2176BT					682:687	2176BT	682:687	2176BT	682:687	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	5	8	theme	clpC	612:615	arg1	genes					617:621	clpC genes	612:621	clpC genes	612:621	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	9	9	dep	nov	1177:1179	arg1	73786T					1206:1211	2177BT=LMG 31471T=CCUG 73786T	1183:1211	nov. (2177BT=LMG 31471T=CCUG 73786T	1177:1211	nov. (2177BT=LMG 31471T=CCUG 73786T are proposed as novel Bifidobacterium species.
32065574	6	10	theme	type	1003:1006	arg1	strains					1008:1014	the type strains	999:1014	the type strains of the so far recognized Bifidobacterium species	999:1063	Further genotyping based on the genome sequence of the isolated strains combined with phenotypic analyses, clearly show that these strains are distinct from each of the type strains of the so far recognized Bifidobacterium species.
32065574	0	11	theme	Bifidobacterium	108:122	arg1	sp					134:135	Bifidobacterium cebidarum sp	108:135	Characterization of the phylogenetic diversity of two novel species belonging to the genus Bifidobacterium: Bifidobacterium cebidarum sp.	0:136	Characterization of the phylogenetic diversity of two novel species belonging to the genus Bifidobacterium: Bifidobacterium cebidarum sp.
32065574	6	12	theme	phenotypic	920:929	arg1	analyses					931:938	phenotypic analyses	920:938	phenotypic analyses	920:938	Further genotyping based on the genome sequence of the isolated strains combined with phenotypic analyses, clearly show that these strains are distinct from each of the type strains of the so far recognized Bifidobacterium species.
32065574	8	13	theme	Bifidobacterium	1143:1157	arg1	sp					1173:1174	Bifidobacterium leontopitheci sp	1143:1174	Bifidobacterium leontopitheci sp	1143:1174	nov. (2176BT=LMG 31469T=CCUG 73785T) and Bifidobacterium leontopitheci sp.
32065574	5	14	theme	DSM	768:770	arg1	103139T					772:778	Bifidobacterium felsineum DSM 103139T	742:778	Bifidobacterium felsineum DSM 103139T	742:778	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	6	15	theme	recognized	1030:1039	arg1	species					1057:1063	the so far recognized Bifidobacterium species	1019:1063	the so far recognized Bifidobacterium species	1019:1063	Further genotyping based on the genome sequence of the isolated strains combined with phenotypic analyses, clearly show that these strains are distinct from each of the type strains of the so far recognized Bifidobacterium species.
32065574	5	16	theme	16S	532:534	arg1	dnaJ					597:600	dnaJ	597:600	dnaJ	597:600	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	5	16	theme	16S	532:534	arg1	hsp60					584:588	hsp60	584:588	hsp60	584:588	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	5	16	theme	16S	532:534	arg1	dnaG					603:606	dnaG	603:606	dnaG	603:606	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	5	16	theme	16S	532:534	arg1	sequences					541:549	16S rRNA sequences	532:549	16S rRNA sequences	532:549	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	5	16	theme	16S	532:534	arg1	genes					617:621	clpC genes	612:621	clpC genes	612:621	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	5	16	theme	16S	532:534	arg1	rpoB					591:594	rpoB	591:594	rpoB	591:594	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	5	16	theme	16S	532:534	arg1	sequences					563:571	multilocus sequences	552:571	multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes)	552:622	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	5	17	dep	strains	674:680	arg1	strains					674:680	bifidobacterial strains	658:680	bifidobacterial strains 2176BT and 2177BT	658:698	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	5	17	dep	strains	674:680	arg1	2177BT					693:698	2177BT	693:698	2177BT	693:698	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	5	17	dep	strains	674:680	arg1	2176BT					682:687	2176BT	682:687	2176BT	682:687	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	9	18	theme	novel	1229:1233	arg1	nov					1177:1179	nov	1177:1179	nov	1177:1179	nov. (2177BT=LMG 31471T=CCUG 73786T are proposed as novel Bifidobacterium species.
32065574	9	18	theme	novel	1229:1233	arg1	species					1251:1257	novel Bifidobacterium species	1229:1257	novel Bifidobacterium species	1229:1257	nov. (2177BT=LMG 31471T=CCUG 73786T are proposed as novel Bifidobacterium species.
32065574	5	19	theme	rRNA	536:539	arg1	dnaJ					597:600	dnaJ	597:600	dnaJ	597:600	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	5	19	theme	rRNA	536:539	arg1	hsp60					584:588	hsp60	584:588	hsp60	584:588	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	5	19	theme	rRNA	536:539	arg1	dnaG					603:606	dnaG	603:606	dnaG	603:606	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	5	19	theme	rRNA	536:539	arg1	sequences					541:549	16S rRNA sequences	532:549	16S rRNA sequences	532:549	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	5	19	theme	rRNA	536:539	arg1	genes					617:621	clpC genes	612:621	clpC genes	612:621	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	5	19	theme	rRNA	536:539	arg1	rpoB					591:594	rpoB	591:594	rpoB	591:594	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	5	19	theme	rRNA	536:539	arg1	sequences					563:571	multilocus sequences	552:571	multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes)	552:622	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	5	20	theme	phylogenetic	714:725	arg1	relatedness					727:737	close phylogenetic relatedness	708:737	close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T	708:817	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	3	21	dep	2176BT	221:226	arg1	i.e.					215:218	i.e.	215:218	i.e.	215:218	Two Bifidobacterium strains, i.e., 2176BT and 2177BT, were isolated from Golden-Headed Lion Tamarin (Leontopithecus chrysomelas) and Goeldi's monkey (Callimico goeldii).
32065574	8	22	theme	31469T=CCUG	1119:1129	arg1	73785T					1131:1136	2176BT=LMG 31469T=CCUG 73785T	1108:1136	2176BT=LMG 31469T=CCUG 73785T	1108:1136	nov. (2176BT=LMG 31469T=CCUG 73785T) and Bifidobacterium leontopitheci sp.
32065574	8	22	theme	31469T=CCUG	1119:1129	arg1	nov					1102:1104	nov	1102:1104	nov	1102:1104	nov. (2176BT=LMG 31469T=CCUG 73785T) and Bifidobacterium leontopitheci sp.
32065574	5	23	theme	core	632:635	arg1	genome					637:642	the core genome	628:642	the core genome	628:642	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	3	24	theme	Bifidobacterium	190:204	arg1	strains					206:212	Two Bifidobacterium strains	186:212	Two Bifidobacterium strains	186:212	Two Bifidobacterium strains, i.e., 2176BT and 2177BT, were isolated from Golden-Headed Lion Tamarin (Leontopithecus chrysomelas) and Goeldi's monkey (Callimico goeldii).
32065574	1	25	dep	sp	177:178	arg1	leontopitheci					163:175	nov. and Bifidobacterium leontopitheci	138:175	nov. and Bifidobacterium leontopitheci	138:175	nov. and Bifidobacterium leontopitheci sp.
32065574	0	26	theme	phylogenetic	24:35	arg1	diversity					37:45	the phylogenetic diversity	20:45	the phylogenetic diversity of two novel species belonging to the genus Bifidobacterium	20:105	Characterization of the phylogenetic diversity of two novel species belonging to the genus Bifidobacterium: Bifidobacterium cebidarum sp.
32065574	9	27	theme	Bifidobacterium	1235:1249	arg1	nov					1177:1179	nov	1177:1179	nov	1177:1179	nov. (2177BT=LMG 31471T=CCUG 73786T are proposed as novel Bifidobacterium species.
32065574	9	27	theme	Bifidobacterium	1235:1249	arg1	species					1251:1257	novel Bifidobacterium species	1229:1257	novel Bifidobacterium species	1229:1257	nov. (2177BT=LMG 31471T=CCUG 73786T are proposed as novel Bifidobacterium species.
32065574	5	28	dep	Bifidobacterium	784:798	arg1	bifidum					800:806	bifidum	800:806	bifidum	800:806	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	7	29	theme	Bifidobacterium	1072:1086	arg1	sp					1098:1099	Bifidobacterium cebidarum sp	1072:1099	Bifidobacterium cebidarum sp	1072:1099	Thus, Bifidobacterium cebidarum sp.
32065574	0	30	theme	cebidarum	124:132	arg1	sp					134:135	Bifidobacterium cebidarum sp	108:135	Characterization of the phylogenetic diversity of two novel species belonging to the genus Bifidobacterium: Bifidobacterium cebidarum sp.	0:136	Characterization of the phylogenetic diversity of two novel species belonging to the genus Bifidobacterium: Bifidobacterium cebidarum sp.
32065574	6	31	theme	strains	898:904	arg1	sequence					873:880	the genome sequence	862:880	the genome sequence of the isolated strains combined with phenotypic analyses	862:938	Further genotyping based on the genome sequence of the isolated strains combined with phenotypic analyses, clearly show that these strains are distinct from each of the type strains of the so far recognized Bifidobacterium species.
32065574	5	32	theme	Phylogenetic	501:512	arg1	analyses					514:521	Phylogenetic analyses	501:521	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes)	501:622	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	5	33	theme	LMG	808:810	arg1	11041T					812:817	Bifidobacterium bifidum LMG 11041T	784:817	Bifidobacterium bifidum LMG 11041T	784:817	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	3	34	theme	Golden-Headed	259:271	arg1	Tamarin					278:284	Golden-Headed Lion Tamarin	259:284	Golden-Headed Lion Tamarin (Leontopithecus chrysomelas)	259:313	Two Bifidobacterium strains, i.e., 2176BT and 2177BT, were isolated from Golden-Headed Lion Tamarin (Leontopithecus chrysomelas) and Goeldi's monkey (Callimico goeldii).
32065574	6	35	theme	isolated	889:896	arg1	strains					898:904	the isolated strains	885:904	the isolated strains combined with phenotypic analyses	885:938	Further genotyping based on the genome sequence of the isolated strains combined with phenotypic analyses, clearly show that these strains are distinct from each of the type strains of the so far recognized Bifidobacterium species.
32065574	0	36	theme	diversity	37:45	arg1	Characterization					0:15	Characterization	0:15	Characterization of the phylogenetic diversity of two novel species belonging to the genus Bifidobacterium: Bifidobacterium cebidarum sp.	0:136	Characterization of the phylogenetic diversity of two novel species belonging to the genus Bifidobacterium: Bifidobacterium cebidarum sp.
32065574	3	37	theme	Lion	273:276	arg1	Tamarin					278:284	Golden-Headed Lion Tamarin	259:284	Golden-Headed Lion Tamarin (Leontopithecus chrysomelas)	259:313	Two Bifidobacterium strains, i.e., 2176BT and 2177BT, were isolated from Golden-Headed Lion Tamarin (Leontopithecus chrysomelas) and Goeldi's monkey (Callimico goeldii).
32065574	6	38	theme	species	1057:1063	arg1	strains					1008:1014	the type strains	999:1014	the type strains of the so far recognized Bifidobacterium species	999:1063	Further genotyping based on the genome sequence of the isolated strains combined with phenotypic analyses, clearly show that these strains are distinct from each of the type strains of the so far recognized Bifidobacterium species.
32065574	0	39	theme	novel	54:58	arg1	species					60:66	two novel species	50:66	two novel species belonging to the genus Bifidobacterium	50:105	Characterization of the phylogenetic diversity of two novel species belonging to the genus Bifidobacterium: Bifidobacterium cebidarum sp.
32065574	3	40	dep	Callimico	336:344	arg1	goeldii					346:352	Callimico goeldii	336:352	Callimico goeldii	336:352	Two Bifidobacterium strains, i.e., 2176BT and 2177BT, were isolated from Golden-Headed Lion Tamarin (Leontopithecus chrysomelas) and Goeldi's monkey (Callimico goeldii).
32065574	4	41	theme	d-fructose	452:461	arg1	6-phosphate					463:473	d-fructose 6-phosphate	452:473	d-fructose 6-phosphate	452:473	Isolates were shown to be Gram-positive, non-motile, non-sporulating, facultative anaerobic and d-fructose 6-phosphate phosphoketolase-positive.
32065574	7	42	theme	cebidarum	1088:1096	arg1	sp					1098:1099	Bifidobacterium cebidarum sp	1072:1099	Bifidobacterium cebidarum sp	1072:1099	Thus, Bifidobacterium cebidarum sp.
32065574	6	43	from	each	991:994	arg1	distinct					977:984	distinct	977:984	distinct	977:984	Further genotyping based on the genome sequence of the isolated strains combined with phenotypic analyses, clearly show that these strains are distinct from each of the type strains of the so far recognized Bifidobacterium species.
32065574	8	44	theme	2176BT=LMG	1108:1117	arg1	73785T					1131:1136	2176BT=LMG 31469T=CCUG 73785T	1108:1136	2176BT=LMG 31469T=CCUG 73785T	1108:1136	nov. (2176BT=LMG 31469T=CCUG 73785T) and Bifidobacterium leontopitheci sp.
32065574	8	44	theme	2176BT=LMG	1108:1117	arg1	nov					1102:1104	nov	1102:1104	nov	1102:1104	nov. (2176BT=LMG 31469T=CCUG 73785T) and Bifidobacterium leontopitheci sp.
32065574	6	45	theme	genome	866:871	arg1	sequence					873:880	the genome sequence	862:880	the genome sequence of the isolated strains combined with phenotypic analyses	862:938	Further genotyping based on the genome sequence of the isolated strains combined with phenotypic analyses, clearly show that these strains are distinct from each of the type strains of the so far recognized Bifidobacterium species.
32065574	6	46	theme	Bifidobacterium	1041:1055	arg1	species					1057:1063	the so far recognized Bifidobacterium species	1019:1063	the so far recognized Bifidobacterium species	1019:1063	Further genotyping based on the genome sequence of the isolated strains combined with phenotypic analyses, clearly show that these strains are distinct from each of the type strains of the so far recognized Bifidobacterium species.
32065574	5	47	dep	Bifidobacterium	742:756	arg1	felsineum					758:766	felsineum	758:766	felsineum	758:766	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	1	48	theme	nov.	138:141	arg1	leontopitheci					163:175	nov. and Bifidobacterium leontopitheci	138:175	nov. and Bifidobacterium leontopitheci	138:175	nov. and Bifidobacterium leontopitheci sp.
32065574	3	49	attach	isolated	245:252	arg1	Tamarin					278:284	Golden-Headed Lion Tamarin	259:284	Golden-Headed Lion Tamarin (Leontopithecus chrysomelas)	259:313	Two Bifidobacterium strains, i.e., 2176BT and 2177BT, were isolated from Golden-Headed Lion Tamarin (Leontopithecus chrysomelas) and Goeldi's monkey (Callimico goeldii).
32065574	3	49	attach	isolated	245:252	arg2	strains					206:212	Two Bifidobacterium strains	186:212	Two Bifidobacterium strains	186:212	Two Bifidobacterium strains, i.e., 2176BT and 2177BT, were isolated from Golden-Headed Lion Tamarin (Leontopithecus chrysomelas) and Goeldi's monkey (Callimico goeldii).
32065574	8	50	theme	leontopitheci	1159:1171	arg1	sp					1173:1174	Bifidobacterium leontopitheci sp	1143:1174	Bifidobacterium leontopitheci sp	1143:1174	nov. (2176BT=LMG 31469T=CCUG 73785T) and Bifidobacterium leontopitheci sp.
32065574	0	51	theme	species	60:66	arg1	diversity					37:45	the phylogenetic diversity	20:45	the phylogenetic diversity of two novel species belonging to the genus Bifidobacterium	20:105	Characterization of the phylogenetic diversity of two novel species belonging to the genus Bifidobacterium: Bifidobacterium cebidarum sp.
32065574	0	52	dep	Characterization	0:15	arg1	sp					134:135	Bifidobacterium cebidarum sp	108:135	Characterization of the phylogenetic diversity of two novel species belonging to the genus Bifidobacterium: Bifidobacterium cebidarum sp.	0:136	Characterization of the phylogenetic diversity of two novel species belonging to the genus Bifidobacterium: Bifidobacterium cebidarum sp.
32065574	5	53	theme	multilocus	552:561	arg1	dnaJ					597:600	dnaJ	597:600	dnaJ	597:600	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	5	53	theme	multilocus	552:561	arg1	hsp60					584:588	hsp60	584:588	hsp60	584:588	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	5	53	theme	multilocus	552:561	arg1	dnaG					603:606	dnaG	603:606	dnaG	603:606	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	5	53	theme	multilocus	552:561	arg1	sequences					541:549	16S rRNA sequences	532:549	16S rRNA sequences	532:549	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	5	53	theme	multilocus	552:561	arg1	genes					617:621	clpC genes	612:621	clpC genes	612:621	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	5	53	theme	multilocus	552:561	arg1	rpoB					591:594	rpoB	591:594	rpoB	591:594	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	5	53	theme	multilocus	552:561	arg1	sequences					563:571	multilocus sequences	552:571	multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes)	552:622	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
32065574	1	54	theme	Bifidobacterium	147:161	arg1	leontopitheci					163:175	nov. and Bifidobacterium leontopitheci	138:175	nov. and Bifidobacterium leontopitheci	138:175	nov. and Bifidobacterium leontopitheci sp.
32065574	5	55	theme	Bifidobacterium	742:756	arg1	103139T					772:778	Bifidobacterium felsineum DSM 103139T	742:778	Bifidobacterium felsineum DSM 103139T	742:778	Phylogenetic analyses based on 16S rRNA sequences, multilocus sequences (including hsp60, rpoB, dnaJ, dnaG and clpC genes) and the core genome revealed that bifidobacterial strains 2176BT and 2177BT exhibit close phylogenetic relatedness to Bifidobacterium felsineum DSM 103139T and Bifidobacterium bifidum LMG 11041T, respectively.
33127381	8	0	theme	CRS1	1098:1101	arg1	O-glycans					1085:1093	the O-glycans	1081:1093	the O-glycans of CRS1 expressed in Paneth cells	1081:1127	Identification of α1,2-fucose on the O-glycans of CRS1 expressed in Paneth cells will pave the way for a mechanistic understanding of α1,2-fucose-dependent symbiosis with intestinal bacteria and elimination of pathogenic bacteria in the intestine.
33127381	5	1	theme	2962 N-linked	738:750	arg1	glycopeptides					752:764	3212 O-linked and 2962 N-linked glycopeptides	720:764	3212 O-linked and 2962 N-linked glycopeptides	720:764	Glycoproteomic analysis of ileal lysates identified 3212 O-linked and 2962 N-linked glycopeptides.
33127381	3	2	theme	intestine	527:535	arg1	cells					508:512	Paneth cells	501:512	Paneth cells of the small intestine	501:535	It was previously reported that Paneth cells of the small intestine were positive for UEA-1 lectin staining.
33127381	8	3	theme	intestinal	1219:1228	arg1	bacteria					1230:1237	intestinal bacteria	1219:1237	intestinal bacteria	1219:1237	Identification of α1,2-fucose on the O-glycans of CRS1 expressed in Paneth cells will pave the way for a mechanistic understanding of α1,2-fucose-dependent symbiosis with intestinal bacteria and elimination of pathogenic bacteria in the intestine.
33127381	5	4	theme	ileal	695:699	arg1	lysates					701:707	ileal lysates	695:707	ileal lysates	695:707	Glycoproteomic analysis of ileal lysates identified 3212 O-linked and 2962 N-linked glycopeptides.
33127381	8	5	from	Identification	1048:1061	arg1	O-glycans					1085:1093	the O-glycans	1081:1093	the O-glycans of CRS1 expressed in Paneth cells	1081:1127	Identification of α1,2-fucose on the O-glycans of CRS1 expressed in Paneth cells will pave the way for a mechanistic understanding of α1,2-fucose-dependent symbiosis with intestinal bacteria and elimination of pathogenic bacteria in the intestine.
33127381	4	6	theme	Paneth	604:609	arg1	cells					611:615	Paneth cells	604:615	Paneth cells carrying α1,2-fucose	604:636	However, glycoproteins in Paneth cells carrying α1,2-fucose have not yet been identified.
33127381	5	7	gly	glycopeptides	752:764	arg2	glycopeptides					752:764	3212 O-linked and 2962 N-linked glycopeptides	720:764	3212 O-linked and 2962 N-linked glycopeptides	720:764	Glycoproteomic analysis of ileal lysates identified 3212 O-linked and 2962 N-linked glycopeptides.
33127381	5	8	theme	lysates	701:707	arg1	analysis					683:690	Glycoproteomic analysis	668:690	Glycoproteomic analysis of ileal lysates	668:707	Glycoproteomic analysis of ileal lysates identified 3212 O-linked and 2962 N-linked glycopeptides.
33127381	7	9	theme	main	1032:1035	arg1	glycoform					1037:1045	the main glycoform	1028:1045	the main glycoform	1028:1045	Unlike other antimicrobial α-defensin proteins, CRS1 contains unique Thr residues, which are modified with O-glycans, with 3HexNAc2Hex1Fuc1NeuAc being the main glycoform.
33127381	7	9	theme	main	1032:1035	arg1	3HexNAc2Hex1Fuc1NeuAc					1000:1020	3HexNAc2Hex1Fuc1NeuAc	1000:1020	3HexNAc2Hex1Fuc1NeuAc	1000:1020	Unlike other antimicrobial α-defensin proteins, CRS1 contains unique Thr residues, which are modified with O-glycans, with 3HexNAc2Hex1Fuc1NeuAc being the main glycoform.
33127381	7	10	theme	unique	939:944	arg1	residues					950:957	unique Thr residues	939:957	unique Thr residues	939:957	Unlike other antimicrobial α-defensin proteins, CRS1 contains unique Thr residues, which are modified with O-glycans, with 3HexNAc2Hex1Fuc1NeuAc being the main glycoform.
33127381	2	11	gly	α1,2-fucosylated	298:313	arg1	mucin					315:319	α1,2-fucosylated mucin	298:319	α1,2-fucosylated mucin secreted from goblet cells	298:346	It is postulated that α1,2-fucosylated mucin secreted from goblet cells is involved in defending an organism against infections, but the detailed molecular mechanisms are yet to be elucidated.
33127381	1	12	with	modification	143:154	arg1	α1,2-fucose					174:184	α1,2-fucose	174:184	α1,2-fucose	174:184	The modification of galactose with α1,2-fucose is involved in symbiosis with intestinal bacteria and elimination of pathogenic bacteria.
33127381	8	13	with	symbiosis	1204:1212	arg1	elimination					1243:1253	elimination	1243:1253	elimination of pathogenic bacteria in the intestine	1243:1293	Identification of α1,2-fucose on the O-glycans of CRS1 expressed in Paneth cells will pave the way for a mechanistic understanding of α1,2-fucose-dependent symbiosis with intestinal bacteria and elimination of pathogenic bacteria in the intestine.
33127381	8	13	with	symbiosis	1204:1212	arg1	bacteria					1230:1237	intestinal bacteria	1219:1237	intestinal bacteria	1219:1237	Identification of α1,2-fucose on the O-glycans of CRS1 expressed in Paneth cells will pave the way for a mechanistic understanding of α1,2-fucose-dependent symbiosis with intestinal bacteria and elimination of pathogenic bacteria in the intestine.
33127381	3	14	theme	UEA-1	555:559	arg1	staining					568:575	UEA-1 lectin staining	555:575	UEA-1 lectin staining	555:575	It was previously reported that Paneth cells of the small intestine were positive for UEA-1 lectin staining.
33127381	0	15	theme	small	122:126	arg1	intestine					128:136	the small intestine	118:136	the small intestine	118:136	Glycoproteomic analysis identifies cryptdin-related sequence 1 as O-glycosylated protein modified with α1,2-fucose in the small intestine.
33127381	8	16	theme	Paneth	1116:1121	arg1	cells					1123:1127	Paneth cells	1116:1127	Paneth cells	1116:1127	Identification of α1,2-fucose on the O-glycans of CRS1 expressed in Paneth cells will pave the way for a mechanistic understanding of α1,2-fucose-dependent symbiosis with intestinal bacteria and elimination of pathogenic bacteria in the intestine.
33127381	2	17	theme	α1,2-fucosylated	298:313	arg1	mucin					315:319	α1,2-fucosylated mucin	298:319	α1,2-fucosylated mucin secreted from goblet cells	298:346	It is postulated that α1,2-fucosylated mucin secreted from goblet cells is involved in defending an organism against infections, but the detailed molecular mechanisms are yet to be elucidated.
33127381	7	18	theme	α-defensin	904:913	arg1	proteins					915:922	other antimicrobial α-defensin proteins	884:922	other antimicrobial α-defensin proteins	884:922	Unlike other antimicrobial α-defensin proteins, CRS1 contains unique Thr residues, which are modified with O-glycans, with 3HexNAc2Hex1Fuc1NeuAc being the main glycoform.
33127381	2	19	theme	molecular	422:430	arg1	mechanisms					432:441	the detailed molecular mechanisms	409:441	the detailed molecular mechanisms	409:441	It is postulated that α1,2-fucosylated mucin secreted from goblet cells is involved in defending an organism against infections, but the detailed molecular mechanisms are yet to be elucidated.
33127381	5	20	theme	Glycoproteomic	668:681	arg1	analysis					683:690	Glycoproteomic analysis	668:690	Glycoproteomic analysis of ileal lysates	668:707	Glycoproteomic analysis of ileal lysates identified 3212 O-linked and 2962 N-linked glycopeptides.
33127381	8	21	theme	α1,2-fucose	1066:1076	arg1	Identification					1048:1061	Identification	1048:1061	Identification of α1,2-fucose on the O-glycans of CRS1 expressed in Paneth cells	1048:1127	Identification of α1,2-fucose on the O-glycans of CRS1 expressed in Paneth cells will pave the way for a mechanistic understanding of α1,2-fucose-dependent symbiosis with intestinal bacteria and elimination of pathogenic bacteria in the intestine.
33127381	8	22	theme	mechanistic	1153:1163	arg1	understanding					1165:1177	a mechanistic understanding	1151:1177	a mechanistic understanding of α1,2-fucose-dependent symbiosis with intestinal bacteria and elimination of pathogenic bacteria in the intestine	1151:1293	Identification of α1,2-fucose on the O-glycans of CRS1 expressed in Paneth cells will pave the way for a mechanistic understanding of α1,2-fucose-dependent symbiosis with intestinal bacteria and elimination of pathogenic bacteria in the intestine.
33127381	2	23	theme	detailed	413:420	arg1	mechanisms					432:441	the detailed molecular mechanisms	409:441	the detailed molecular mechanisms	409:441	It is postulated that α1,2-fucosylated mucin secreted from goblet cells is involved in defending an organism against infections, but the detailed molecular mechanisms are yet to be elucidated.
33127381	0	24	theme	Glycoproteomic	0:13	arg1	analysis					15:22	Glycoproteomic analysis	0:22	Glycoproteomic analysis	0:22	Glycoproteomic analysis identifies cryptdin-related sequence 1 as O-glycosylated protein modified with α1,2-fucose in the small intestine.
33127381	6	25	theme	Paneth	830:835	arg1	cells					837:841	Paneth cells	830:841	Paneth cells	830:841	In particular, cryptdin-related sequence 1 (CRS1) expressed in Paneth cells was found to be α1,2-fucosylated.
33127381	7	26	theme	other	884:888	arg1	proteins					915:922	other antimicrobial α-defensin proteins	884:922	other antimicrobial α-defensin proteins	884:922	Unlike other antimicrobial α-defensin proteins, CRS1 contains unique Thr residues, which are modified with O-glycans, with 3HexNAc2Hex1Fuc1NeuAc being the main glycoform.
33127381	7	27	contain	contains	930:937	arg2	residues					950:957	unique Thr residues	939:957	unique Thr residues	939:957	Unlike other antimicrobial α-defensin proteins, CRS1 contains unique Thr residues, which are modified with O-glycans, with 3HexNAc2Hex1Fuc1NeuAc being the main glycoform.
33127381	7	27	contain	contains	930:937	arg1	CRS1					925:928	CRS1	925:928	CRS1	925:928	Unlike other antimicrobial α-defensin proteins, CRS1 contains unique Thr residues, which are modified with O-glycans, with 3HexNAc2Hex1Fuc1NeuAc being the main glycoform.
33127381	0	28	theme	cryptdin-related	35:50	arg1	sequence					52:59	cryptdin-related sequence 1	35:61	cryptdin-related sequence 1	35:61	Glycoproteomic analysis identifies cryptdin-related sequence 1 as O-glycosylated protein modified with α1,2-fucose in the small intestine.
33127381	4	29	from	glycoproteins	587:599	arg1	cells					611:615	Paneth cells	604:615	Paneth cells carrying α1,2-fucose	604:636	However, glycoproteins in Paneth cells carrying α1,2-fucose have not yet been identified.
33127381	7	30	theme	antimicrobial	890:902	arg1	proteins					915:922	other antimicrobial α-defensin proteins	884:922	other antimicrobial α-defensin proteins	884:922	Unlike other antimicrobial α-defensin proteins, CRS1 contains unique Thr residues, which are modified with O-glycans, with 3HexNAc2Hex1Fuc1NeuAc being the main glycoform.
33127381	1	31	theme	intestinal	216:225	arg1	bacteria					227:234	intestinal bacteria	216:234	intestinal bacteria	216:234	The modification of galactose with α1,2-fucose is involved in symbiosis with intestinal bacteria and elimination of pathogenic bacteria.
33127381	1	32	with	elimination	240:250	arg1	bacteria					227:234	intestinal bacteria	216:234	intestinal bacteria	216:234	The modification of galactose with α1,2-fucose is involved in symbiosis with intestinal bacteria and elimination of pathogenic bacteria.
33127381	8	33	from	bacteria	1230:1237	arg1	intestine					1285:1293	the intestine	1281:1293	the intestine	1281:1293	Identification of α1,2-fucose on the O-glycans of CRS1 expressed in Paneth cells will pave the way for a mechanistic understanding of α1,2-fucose-dependent symbiosis with intestinal bacteria and elimination of pathogenic bacteria in the intestine.
33127381	2	34	theme	goblet	335:340	arg1	cells					342:346	goblet cells	335:346	goblet cells	335:346	It is postulated that α1,2-fucosylated mucin secreted from goblet cells is involved in defending an organism against infections, but the detailed molecular mechanisms are yet to be elucidated.
33127381	7	35	mod	modified	970:977	arg1	residues					950:957	unique Thr residues	939:957	unique Thr residues	939:957	Unlike other antimicrobial α-defensin proteins, CRS1 contains unique Thr residues, which are modified with O-glycans, with 3HexNAc2Hex1Fuc1NeuAc being the main glycoform.
33127381	7	35	mod	modified	970:977	arg3	O-glycans					984:992	O-glycans	984:992	O-glycans	984:992	Unlike other antimicrobial α-defensin proteins, CRS1 contains unique Thr residues, which are modified with O-glycans, with 3HexNAc2Hex1Fuc1NeuAc being the main glycoform.
33127381	3	36	theme	Paneth	501:506	arg1	cells					508:512	Paneth cells	501:512	Paneth cells of the small intestine	501:535	It was previously reported that Paneth cells of the small intestine were positive for UEA-1 lectin staining.
33127381	5	37	link	2962 N-linked	738:750	arg1	glycopeptides					752:764	3212 O-linked and 2962 N-linked glycopeptides	720:764	3212 O-linked and 2962 N-linked glycopeptides	720:764	Glycoproteomic analysis of ileal lysates identified 3212 O-linked and 2962 N-linked glycopeptides.
33127381	4	38	contain	carrying	617:624	arg2	α1,2-fucose					626:636	α1,2-fucose	626:636	α1,2-fucose	626:636	However, glycoproteins in Paneth cells carrying α1,2-fucose have not yet been identified.
33127381	4	38	contain	carrying	617:624	arg1	cells					611:615	Paneth cells	604:615	Paneth cells carrying α1,2-fucose	604:636	However, glycoproteins in Paneth cells carrying α1,2-fucose have not yet been identified.
33127381	6	39	located	found	847:851	arg2	CRS1					811:814	CRS1	811:814	CRS1	811:814	In particular, cryptdin-related sequence 1 (CRS1) expressed in Paneth cells was found to be α1,2-fucosylated.
33127381	6	39	located	found	847:851	arg2	sequence					799:806	cryptdin-related sequence 1	782:808	cryptdin-related sequence 1 (CRS1) expressed in Paneth cells	782:841	In particular, cryptdin-related sequence 1 (CRS1) expressed in Paneth cells was found to be α1,2-fucosylated.
33127381	6	39	located	found	847:851	arg1	particular					770:779	particular	770:779	particular	770:779	In particular, cryptdin-related sequence 1 (CRS1) expressed in Paneth cells was found to be α1,2-fucosylated.
33127381	0	40	theme	O-glycosylated	66:79	arg1	protein					81:87	O-glycosylated protein	66:87	O-glycosylated protein modified with α1,2-fucose in the small intestine	66:136	Glycoproteomic analysis identifies cryptdin-related sequence 1 as O-glycosylated protein modified with α1,2-fucose in the small intestine.
33127381	6	41	gly	α1,2-fucosylated	859:874	arg1	sequence					799:806	cryptdin-related sequence 1	782:808	cryptdin-related sequence 1 (CRS1) expressed in Paneth cells	782:841	In particular, cryptdin-related sequence 1 (CRS1) expressed in Paneth cells was found to be α1,2-fucosylated.
33127381	6	41	gly	α1,2-fucosylated	859:874	arg1	CRS1					811:814	CRS1	811:814	CRS1	811:814	In particular, cryptdin-related sequence 1 (CRS1) expressed in Paneth cells was found to be α1,2-fucosylated.
33127381	1	42	with	symbiosis	201:209	arg1	bacteria					227:234	intestinal bacteria	216:234	intestinal bacteria	216:234	The modification of galactose with α1,2-fucose is involved in symbiosis with intestinal bacteria and elimination of pathogenic bacteria.
33127381	5	43	theme	O-linked	725:732	arg1	glycopeptides					752:764	3212 O-linked and 2962 N-linked glycopeptides	720:764	3212 O-linked and 2962 N-linked glycopeptides	720:764	Glycoproteomic analysis of ileal lysates identified 3212 O-linked and 2962 N-linked glycopeptides.
33127381	4	44	gly	glycoproteins	587:599	arg1	glycoproteins					587:599	glycoproteins	587:599	glycoproteins in Paneth cells carrying α1,2-fucose	587:636	However, glycoproteins in Paneth cells carrying α1,2-fucose have not yet been identified.
33127381	3	45	theme	small	521:525	arg1	intestine					527:535	the small intestine	517:535	the small intestine	517:535	It was previously reported that Paneth cells of the small intestine were positive for UEA-1 lectin staining.
33127381	8	46	theme	α1,2-fucose-dependent	1182:1202	arg1	symbiosis					1204:1212	α1,2-fucose-dependent symbiosis	1182:1212	α1,2-fucose-dependent symbiosis with intestinal bacteria and elimination of pathogenic bacteria in the intestine	1182:1293	Identification of α1,2-fucose on the O-glycans of CRS1 expressed in Paneth cells will pave the way for a mechanistic understanding of α1,2-fucose-dependent symbiosis with intestinal bacteria and elimination of pathogenic bacteria in the intestine.
33127381	3	47	theme	lectin	561:566	arg1	staining					568:575	UEA-1 lectin staining	555:575	UEA-1 lectin staining	555:575	It was previously reported that Paneth cells of the small intestine were positive for UEA-1 lectin staining.
33127381	8	48	from	elimination	1243:1253	arg1	intestine					1285:1293	the intestine	1281:1293	the intestine	1281:1293	Identification of α1,2-fucose on the O-glycans of CRS1 expressed in Paneth cells will pave the way for a mechanistic understanding of α1,2-fucose-dependent symbiosis with intestinal bacteria and elimination of pathogenic bacteria in the intestine.
33127381	8	49	theme	bacteria	1269:1276	arg1	elimination					1243:1253	elimination	1243:1253	elimination of pathogenic bacteria in the intestine	1243:1293	Identification of α1,2-fucose on the O-glycans of CRS1 expressed in Paneth cells will pave the way for a mechanistic understanding of α1,2-fucose-dependent symbiosis with intestinal bacteria and elimination of pathogenic bacteria in the intestine.
33127381	8	49	theme	bacteria	1269:1276	arg1	bacteria					1230:1237	intestinal bacteria	1219:1237	intestinal bacteria	1219:1237	Identification of α1,2-fucose on the O-glycans of CRS1 expressed in Paneth cells will pave the way for a mechanistic understanding of α1,2-fucose-dependent symbiosis with intestinal bacteria and elimination of pathogenic bacteria in the intestine.
33127381	5	50	link	O-linked	725:732	arg1	glycopeptides					752:764	3212 O-linked and 2962 N-linked glycopeptides	720:764	3212 O-linked and 2962 N-linked glycopeptides	720:764	Glycoproteomic analysis of ileal lysates identified 3212 O-linked and 2962 N-linked glycopeptides.
33127381	1	51	theme	pathogenic	255:264	arg1	bacteria					266:273	pathogenic bacteria	255:273	pathogenic bacteria	255:273	The modification of galactose with α1,2-fucose is involved in symbiosis with intestinal bacteria and elimination of pathogenic bacteria.
33127381	8	52	theme	pathogenic	1258:1267	arg1	bacteria					1269:1276	pathogenic bacteria	1258:1276	pathogenic bacteria	1258:1276	Identification of α1,2-fucose on the O-glycans of CRS1 expressed in Paneth cells will pave the way for a mechanistic understanding of α1,2-fucose-dependent symbiosis with intestinal bacteria and elimination of pathogenic bacteria in the intestine.
33127381	1	53	theme	galactose	159:167	arg1	modification					143:154	The modification	139:154	The modification of galactose with α1,2-fucose	139:184	The modification of galactose with α1,2-fucose is involved in symbiosis with intestinal bacteria and elimination of pathogenic bacteria.
33127381	1	54	theme	bacteria	266:273	arg1	symbiosis					201:209	symbiosis	201:209	symbiosis with intestinal bacteria	201:234	The modification of galactose with α1,2-fucose is involved in symbiosis with intestinal bacteria and elimination of pathogenic bacteria.
33127381	1	54	theme	bacteria	266:273	arg1	elimination					240:250	elimination	240:250	elimination of pathogenic bacteria	240:273	The modification of galactose with α1,2-fucose is involved in symbiosis with intestinal bacteria and elimination of pathogenic bacteria.
33127381	8	55	theme	symbiosis	1204:1212	arg1	understanding					1165:1177	a mechanistic understanding	1151:1177	a mechanistic understanding of α1,2-fucose-dependent symbiosis with intestinal bacteria and elimination of pathogenic bacteria in the intestine	1151:1293	Identification of α1,2-fucose on the O-glycans of CRS1 expressed in Paneth cells will pave the way for a mechanistic understanding of α1,2-fucose-dependent symbiosis with intestinal bacteria and elimination of pathogenic bacteria in the intestine.
33127381	0	56	gly	O-glycosylated	66:79	arg1	protein					81:87	O-glycosylated protein	66:87	O-glycosylated protein modified with α1,2-fucose in the small intestine	66:136	Glycoproteomic analysis identifies cryptdin-related sequence 1 as O-glycosylated protein modified with α1,2-fucose in the small intestine.
33127381	6	57	theme	cryptdin-related	782:797	arg1	sequence					799:806	cryptdin-related sequence 1	782:808	cryptdin-related sequence 1 (CRS1) expressed in Paneth cells	782:841	In particular, cryptdin-related sequence 1 (CRS1) expressed in Paneth cells was found to be α1,2-fucosylated.
33127381	6	57	theme	cryptdin-related	782:797	arg1	CRS1					811:814	CRS1	811:814	CRS1	811:814	In particular, cryptdin-related sequence 1 (CRS1) expressed in Paneth cells was found to be α1,2-fucosylated.
33127381	7	58	theme	Thr	946:948	arg1	residues					950:957	unique Thr residues	939:957	unique Thr residues	939:957	Unlike other antimicrobial α-defensin proteins, CRS1 contains unique Thr residues, which are modified with O-glycans, with 3HexNAc2Hex1Fuc1NeuAc being the main glycoform.
33498853	0	0	theme	C57BL/6J	85:92	arg1	Mice					94:97	C57BL/6J Mice	85:97	C57BL/6J Mice	85:97	Kale Attenuates Inflammation and Modulates Gut Microbial Composition and Function in C57BL/6J Mice with Diet-Induced Obesity.
33498853	13	1	theme	xenobiotic	1726:1735	arg1	metabolism					1737:1746	xenobiotic metabolism	1726:1746	xenobiotic metabolism	1726:1746	Compared to the HFD, kale supplementation enhanced several bacterial metabolic functions, including glycan degradation, thiamine metabolism and xenobiotic metabolism.
33498853	12	2	theme	digesting	1563:1571	arg1	enzymes					1573:1579	plant polysaccharide digesting enzymes	1542:1579	plant polysaccharide digesting enzymes	1542:1579	This species produces plant polysaccharide digesting enzymes.
33498853	6	3	theme	cecal	800:804	arg1	composition					817:827	cecal microbiota composition	800:827	cecal microbiota composition	800:827	In addition, kale modulated HFD-induced changes in cecal microbiota composition.
33498853	10	4	theme	p	1385:1385	arg1	HFD					1380:1382	the HFD	1376:1382	the HFD (p < 0.05)	1376:1393	Within Actinobacteria, HFKV particularly increased Coriobacteriales/Coriobacteriaceae about four-fold compared to the HFD (p < 0.05).
33498853	10	4	theme	p	1385:1385	arg1	<					1387:1387	p < 0.05	1385:1392	p < 0.05	1385:1392	Within Actinobacteria, HFKV particularly increased Coriobacteriales/Coriobacteriaceae about four-fold compared to the HFD (p < 0.05).
33498853	3	5	from	kale	376:379	arg1	mice					400:403	C57BL/6J mice	391:403	C57BL/6J mice	391:403	We investigated the effect of supplementing a high-fat diet (HFD) with kale (HFKV) in C57BL/6J mice.
33498853	2	6	theme	cruciferous	263:273	arg1	broccoli					295:302	broccoli	295:302	broccoli	295:302	acephala) is a vegetable common in most cultures but is less studied as a functional food compared to other cruciferous vegetables, such as broccoli.
33498853	2	6	theme	cruciferous	263:273	arg1	vegetables					275:284	other cruciferous vegetables	257:284	other cruciferous vegetables	257:284	acephala) is a vegetable common in most cultures but is less studied as a functional food compared to other cruciferous vegetables, such as broccoli.
33498853	12	7	theme	plant	1542:1546	arg1	enzymes					1573:1579	plant polysaccharide digesting enzymes	1542:1579	plant polysaccharide digesting enzymes	1542:1579	This species produces plant polysaccharide digesting enzymes.
33498853	2	8	from	common	180:185	arg1	cultures					195:202	most cultures	190:202	most cultures	190:202	acephala) is a vegetable common in most cultures but is less studied as a functional food compared to other cruciferous vegetables, such as broccoli.
33498853	0	9	from	Composition	57:67	arg1	Mice					94:97	C57BL/6J Mice	85:97	C57BL/6J Mice	85:97	Kale Attenuates Inflammation and Modulates Gut Microbial Composition and Function in C57BL/6J Mice with Diet-Induced Obesity.
33498853	1	10	dep	Brassica	132:139	arg1	oleracea					141:148	Brassica oleracea	132:148	Brassica oleracea	132:148	Kale (Brassica oleracea var.
33498853	0	11	with	Function	73:80	arg1	Obesity					117:123	Diet-Induced Obesity	104:123	Diet-Induced Obesity	104:123	Kale Attenuates Inflammation and Modulates Gut Microbial Composition and Function in C57BL/6J Mice with Diet-Induced Obesity.
33498853	2	12	theme	other	257:261	arg1	broccoli					295:302	broccoli	295:302	broccoli	295:302	acephala) is a vegetable common in most cultures but is less studied as a functional food compared to other cruciferous vegetables, such as broccoli.
33498853	2	12	theme	other	257:261	arg1	vegetables					275:284	other cruciferous vegetables	257:284	other cruciferous vegetables	257:284	acephala) is a vegetable common in most cultures but is less studied as a functional food compared to other cruciferous vegetables, such as broccoli.
33498853	7	13	theme	bacterial	846:854	arg1	diversity					856:864	bacterial diversity	846:864	bacterial diversity	846:864	The HFD lowered bacterial diversity, increased the Firmicutes: Bacteroidetes (F/B) ratio and altered composition.
33498853	11	14	theme	Bacteroides	1442:1452	arg1	thetaiotaomicron					1454:1469	the species Bacteroides thetaiotaomicron	1430:1469	the species Bacteroides thetaiotaomicron	1430:1469	Among Bacteroidia, HFKV increased the species Bacteroides thetaiotaomicron by over two-fold (p = 0.05) compared to the HFD.
33498853	14	15	theme	functional	1794:1803	arg1	food					1805:1808	a functional food	1792:1808	a functional food that modulates the microbiota and changes in inflammation phenotype	1792:1876	Our findings provide evidence that kale is a functional food that modulates the microbiota and changes in inflammation phenotype.
33498853	14	15	theme	functional	1794:1803	arg1	kale					1784:1787	kale	1784:1787	kale	1784:1787	Our findings provide evidence that kale is a functional food that modulates the microbiota and changes in inflammation phenotype.
33498853	4	16	from	role	435:438	arg1	parameters					453:462	metabolic parameters	443:462	metabolic parameters	443:462	We particularly explored its role in metabolic parameters, gut bacterial composition and diversity using 16S rRNA sequencing, systematically compared changes under each phylum and predicted the functional potential of the altered bacterial community using PICRUSt2.
33498853	6	17	theme	HFD-induced	777:787	arg1	changes					789:795	HFD-induced changes	777:795	HFD-induced changes in cecal microbiota composition	777:827	In addition, kale modulated HFD-induced changes in cecal microbiota composition.
33498853	6	18	from	changes	789:795	arg1	composition					817:827	cecal microbiota composition	800:827	cecal microbiota composition	800:827	In addition, kale modulated HFD-induced changes in cecal microbiota composition.
33498853	0	19	theme	Diet-Induced	104:115	arg1	Obesity					117:123	Diet-Induced Obesity	104:123	Diet-Induced Obesity	104:123	Kale Attenuates Inflammation and Modulates Gut Microbial Composition and Function in C57BL/6J Mice with Diet-Induced Obesity.
33498853	13	20	theme	thiamine	1702:1709	arg1	metabolism					1711:1720	thiamine metabolism	1702:1720	thiamine metabolism	1702:1720	Compared to the HFD, kale supplementation enhanced several bacterial metabolic functions, including glycan degradation, thiamine metabolism and xenobiotic metabolism.
33498853	9	21	theme	class	1195:1199	arg1	Bacilli					1201:1207	class Bacilli	1195:1207	class Bacilli	1195:1207	Kale supplementation lowered the F/B ratio, increased both alpha and beta diversity and reduced class Bacilli and Erysipelotrichi but had no effect on Clostridia.
33498853	1	22	dep	Kale	126:129	arg1	Brassica					132:139	Brassica	132:139	Brassica	132:139	Kale (Brassica oleracea var.
33498853	11	23	theme	species	1434:1440	arg1	thetaiotaomicron					1454:1469	the species Bacteroides thetaiotaomicron	1430:1469	the species Bacteroides thetaiotaomicron	1430:1469	Among Bacteroidia, HFKV increased the species Bacteroides thetaiotaomicron by over two-fold (p = 0.05) compared to the HFD.
33498853	14	24	from	microbiota	1829:1838	arg1	inflammation					1855:1866	inflammation	1855:1866	inflammation	1855:1866	Our findings provide evidence that kale is a functional food that modulates the microbiota and changes in inflammation phenotype.
33498853	7	25	theme	altered	923:929	arg1	Firmicutes					881:890	the Firmicutes	877:890	the Firmicutes: Bacteroidetes (F/B) ratio and altered composition	877:941	The HFD lowered bacterial diversity, increased the Firmicutes: Bacteroidetes (F/B) ratio and altered composition.
33498853	7	25	theme	altered	923:929	arg1	composition					931:941	altered composition	923:941	altered composition	923:941	The HFD lowered bacterial diversity, increased the Firmicutes: Bacteroidetes (F/B) ratio and altered composition.
33498853	8	26	dep	Bacteroidetes	988:1000	arg1	Prevotellaceae					1034:1047	Prevotellaceae	1034:1047	Prevotellaceae	1034:1047	Specifically, it lowered Actinobacteria and Bacteroidetes (Bacteroidia, Rikenellaceae and Prevotellaceae) but increased Firmicutes (mainly class Bacilli).
33498853	8	26	dep	Bacteroidetes	988:1000	arg1	Rikenellaceae					1016:1028	Rikenellaceae	1016:1028	Rikenellaceae	1016:1028	Specifically, it lowered Actinobacteria and Bacteroidetes (Bacteroidia, Rikenellaceae and Prevotellaceae) but increased Firmicutes (mainly class Bacilli).
33498853	8	26	dep	Bacteroidetes	988:1000	arg1	Bacteroidia					1003:1013	Bacteroidia	1003:1013	Bacteroidia	1003:1013	Specifically, it lowered Actinobacteria and Bacteroidetes (Bacteroidia, Rikenellaceae and Prevotellaceae) but increased Firmicutes (mainly class Bacilli).
33498853	8	26	dep	Bacteroidetes	988:1000	arg1	Bacteroidetes					988:1000	Bacteroidetes	988:1000	Bacteroidetes (Bacteroidia, Rikenellaceae and Prevotellaceae)	988:1048	Specifically, it lowered Actinobacteria and Bacteroidetes (Bacteroidia, Rikenellaceae and Prevotellaceae) but increased Firmicutes (mainly class Bacilli).
33498853	4	27	theme	altered	628:634	arg1	community					646:654	the altered bacterial community	624:654	the altered bacterial community	624:654	We particularly explored its role in metabolic parameters, gut bacterial composition and diversity using 16S rRNA sequencing, systematically compared changes under each phylum and predicted the functional potential of the altered bacterial community using PICRUSt2.
33498853	13	28	theme	glycan	1682:1687	arg1	degradation					1689:1699	glycan degradation	1682:1699	glycan degradation	1682:1699	Compared to the HFD, kale supplementation enhanced several bacterial metabolic functions, including glycan degradation, thiamine metabolism and xenobiotic metabolism.
33498853	8	29	theme	class	1083:1087	arg1	Bacilli					1089:1095	class Bacilli	1083:1095	mainly class Bacilli	1076:1095	Specifically, it lowered Actinobacteria and Bacteroidetes (Bacteroidia, Rikenellaceae and Prevotellaceae) but increased Firmicutes (mainly class Bacilli).
33498853	7	30	dep	Firmicutes	881:890	arg1	Firmicutes					881:890	the Firmicutes	877:890	the Firmicutes: Bacteroidetes (F/B) ratio and altered composition	877:941	The HFD lowered bacterial diversity, increased the Firmicutes: Bacteroidetes (F/B) ratio and altered composition.
33498853	7	30	dep	Firmicutes	881:890	arg1	ratio					913:917	Bacteroidetes (F/B) ratio	893:917	Bacteroidetes (F/B) ratio	893:917	The HFD lowered bacterial diversity, increased the Firmicutes: Bacteroidetes (F/B) ratio and altered composition.
33498853	7	30	dep	Firmicutes	881:890	arg1	composition					931:941	altered composition	923:941	altered composition	923:941	The HFD lowered bacterial diversity, increased the Firmicutes: Bacteroidetes (F/B) ratio and altered composition.
33498853	9	31	theme	beta	1168:1171	arg1	diversity					1173:1181	both alpha and beta diversity	1153:1181	both alpha and beta diversity	1153:1181	Kale supplementation lowered the F/B ratio, increased both alpha and beta diversity and reduced class Bacilli and Erysipelotrichi but had no effect on Clostridia.
33498853	13	32	theme	kale	1603:1606	arg1	supplementation					1608:1622	kale supplementation	1603:1622	kale supplementation	1603:1622	Compared to the HFD, kale supplementation enhanced several bacterial metabolic functions, including glycan degradation, thiamine metabolism and xenobiotic metabolism.
33498853	3	33	theme	high-fat	351:358	arg1	diet					360:363	a high-fat diet	349:363	a high-fat diet (HFD)	349:369	We investigated the effect of supplementing a high-fat diet (HFD) with kale (HFKV) in C57BL/6J mice.
33498853	3	33	theme	high-fat	351:358	arg1	HFD					366:368	HFD	366:368	HFD	366:368	We investigated the effect of supplementing a high-fat diet (HFD) with kale (HFKV) in C57BL/6J mice.
33498853	4	34	theme	rRNA	515:518	arg1	sequencing					520:529	16S rRNA sequencing	511:529	16S rRNA sequencing	511:529	We particularly explored its role in metabolic parameters, gut bacterial composition and diversity using 16S rRNA sequencing, systematically compared changes under each phylum and predicted the functional potential of the altered bacterial community using PICRUSt2.
33498853	2	35	theme	vegetable	170:178	arg1	common					180:185	a vegetable common	168:185	a vegetable common	168:185	acephala) is a vegetable common in most cultures but is less studied as a functional food compared to other cruciferous vegetables, such as broccoli.
33498853	2	35	theme	vegetable	170:178	arg1	acephala					155:162	acephala	155:162	acephala	155:162	acephala) is a vegetable common in most cultures but is less studied as a functional food compared to other cruciferous vegetables, such as broccoli.
33498853	1	36	dep	var	150:152	arg1	Kale					126:129	Kale	126:129	Kale	126:129	Kale (Brassica oleracea var.
33498853	0	37	theme	Gut	43:45	arg1	Composition					57:67	Gut Microbial Composition	43:67	Gut Microbial Composition	43:67	Kale Attenuates Inflammation and Modulates Gut Microbial Composition and Function in C57BL/6J Mice with Diet-Induced Obesity.
33498853	4	38	theme	16S	511:513	arg1	sequencing					520:529	16S rRNA sequencing	511:529	16S rRNA sequencing	511:529	We particularly explored its role in metabolic parameters, gut bacterial composition and diversity using 16S rRNA sequencing, systematically compared changes under each phylum and predicted the functional potential of the altered bacterial community using PICRUSt2.
33498853	5	39	theme	other	677:681	arg1	vegetables					695:704	other cruciferous vegetables	677:704	other cruciferous vegetables	677:704	Like other cruciferous vegetables, kale attenuated HFD-induced inflammation.
33498853	4	40	theme	functional	600:609	arg1	potential					611:619	the functional potential	596:619	the functional potential of the altered bacterial community	596:654	We particularly explored its role in metabolic parameters, gut bacterial composition and diversity using 16S rRNA sequencing, systematically compared changes under each phylum and predicted the functional potential of the altered bacterial community using PICRUSt2.
33498853	3	41	theme	C57BL/6J	391:398	arg1	mice					400:403	C57BL/6J mice	391:403	C57BL/6J mice	391:403	We investigated the effect of supplementing a high-fat diet (HFD) with kale (HFKV) in C57BL/6J mice.
33498853	4	42	from	composition	479:489	arg1	parameters					453:462	metabolic parameters	443:462	metabolic parameters	443:462	We particularly explored its role in metabolic parameters, gut bacterial composition and diversity using 16S rRNA sequencing, systematically compared changes under each phylum and predicted the functional potential of the altered bacterial community using PICRUSt2.
33498853	4	43	theme	bacterial	469:477	arg1	composition					479:489	gut bacterial composition	465:489	gut bacterial composition	465:489	We particularly explored its role in metabolic parameters, gut bacterial composition and diversity using 16S rRNA sequencing, systematically compared changes under each phylum and predicted the functional potential of the altered bacterial community using PICRUSt2.
33498853	5	44	theme	cruciferous	683:693	arg1	vegetables					695:704	other cruciferous vegetables	677:704	other cruciferous vegetables	677:704	Like other cruciferous vegetables, kale attenuated HFD-induced inflammation.
33498853	0	45	from	Function	73:80	arg1	Mice					94:97	C57BL/6J Mice	85:97	C57BL/6J Mice	85:97	Kale Attenuates Inflammation and Modulates Gut Microbial Composition and Function in C57BL/6J Mice with Diet-Induced Obesity.
33498853	14	46	from	changes	1844:1850	arg1	inflammation					1855:1866	inflammation	1855:1866	inflammation	1855:1866	Our findings provide evidence that kale is a functional food that modulates the microbiota and changes in inflammation phenotype.
33498853	4	47	theme	gut	465:467	arg1	composition					479:489	gut bacterial composition	465:489	gut bacterial composition	465:489	We particularly explored its role in metabolic parameters, gut bacterial composition and diversity using 16S rRNA sequencing, systematically compared changes under each phylum and predicted the functional potential of the altered bacterial community using PICRUSt2.
33498853	13	48	theme	several	1633:1639	arg1	metabolism					1711:1720	thiamine metabolism	1702:1720	thiamine metabolism	1702:1720	Compared to the HFD, kale supplementation enhanced several bacterial metabolic functions, including glycan degradation, thiamine metabolism and xenobiotic metabolism.
33498853	13	48	theme	several	1633:1639	arg1	metabolism					1737:1746	xenobiotic metabolism	1726:1746	xenobiotic metabolism	1726:1746	Compared to the HFD, kale supplementation enhanced several bacterial metabolic functions, including glycan degradation, thiamine metabolism and xenobiotic metabolism.
33498853	13	48	theme	several	1633:1639	arg1	functions					1661:1669	several bacterial metabolic functions	1633:1669	several bacterial metabolic functions	1633:1669	Compared to the HFD, kale supplementation enhanced several bacterial metabolic functions, including glycan degradation, thiamine metabolism and xenobiotic metabolism.
33498853	13	48	theme	several	1633:1639	arg1	degradation					1689:1699	glycan degradation	1682:1699	glycan degradation	1682:1699	Compared to the HFD, kale supplementation enhanced several bacterial metabolic functions, including glycan degradation, thiamine metabolism and xenobiotic metabolism.
33498853	7	49	dep	lowered	838:844	arg1	increased					867:875	increased	867:875	increased the Firmicutes: Bacteroidetes (F/B) ratio and altered composition	867:941	The HFD lowered bacterial diversity, increased the Firmicutes: Bacteroidetes (F/B) ratio and altered composition.
33498853	0	50	theme	Microbial	47:55	arg1	Composition					57:67	Gut Microbial Composition	43:67	Gut Microbial Composition	43:67	Kale Attenuates Inflammation and Modulates Gut Microbial Composition and Function in C57BL/6J Mice with Diet-Induced Obesity.
33498853	13	51	theme	bacterial	1641:1649	arg1	metabolism					1711:1720	thiamine metabolism	1702:1720	thiamine metabolism	1702:1720	Compared to the HFD, kale supplementation enhanced several bacterial metabolic functions, including glycan degradation, thiamine metabolism and xenobiotic metabolism.
33498853	13	51	theme	bacterial	1641:1649	arg1	metabolism					1737:1746	xenobiotic metabolism	1726:1746	xenobiotic metabolism	1726:1746	Compared to the HFD, kale supplementation enhanced several bacterial metabolic functions, including glycan degradation, thiamine metabolism and xenobiotic metabolism.
33498853	13	51	theme	bacterial	1641:1649	arg1	functions					1661:1669	several bacterial metabolic functions	1633:1669	several bacterial metabolic functions	1633:1669	Compared to the HFD, kale supplementation enhanced several bacterial metabolic functions, including glycan degradation, thiamine metabolism and xenobiotic metabolism.
33498853	13	51	theme	bacterial	1641:1649	arg1	degradation					1689:1699	glycan degradation	1682:1699	glycan degradation	1682:1699	Compared to the HFD, kale supplementation enhanced several bacterial metabolic functions, including glycan degradation, thiamine metabolism and xenobiotic metabolism.
33498853	13	52	theme	metabolic	1651:1659	arg1	metabolism					1711:1720	thiamine metabolism	1702:1720	thiamine metabolism	1702:1720	Compared to the HFD, kale supplementation enhanced several bacterial metabolic functions, including glycan degradation, thiamine metabolism and xenobiotic metabolism.
33498853	13	52	theme	metabolic	1651:1659	arg1	metabolism					1737:1746	xenobiotic metabolism	1726:1746	xenobiotic metabolism	1726:1746	Compared to the HFD, kale supplementation enhanced several bacterial metabolic functions, including glycan degradation, thiamine metabolism and xenobiotic metabolism.
33498853	13	52	theme	metabolic	1651:1659	arg1	functions					1661:1669	several bacterial metabolic functions	1633:1669	several bacterial metabolic functions	1633:1669	Compared to the HFD, kale supplementation enhanced several bacterial metabolic functions, including glycan degradation, thiamine metabolism and xenobiotic metabolism.
33498853	13	52	theme	metabolic	1651:1659	arg1	degradation					1689:1699	glycan degradation	1682:1699	glycan degradation	1682:1699	Compared to the HFD, kale supplementation enhanced several bacterial metabolic functions, including glycan degradation, thiamine metabolism and xenobiotic metabolism.
33498853	2	53	theme	most	190:193	arg1	cultures					195:202	most cultures	190:202	most cultures	190:202	acephala) is a vegetable common in most cultures but is less studied as a functional food compared to other cruciferous vegetables, such as broccoli.
33498853	7	54	theme	F/B	908:910	arg1	Firmicutes					881:890	the Firmicutes	877:890	the Firmicutes: Bacteroidetes (F/B) ratio and altered composition	877:941	The HFD lowered bacterial diversity, increased the Firmicutes: Bacteroidetes (F/B) ratio and altered composition.
33498853	7	54	theme	F/B	908:910	arg1	ratio					913:917	Bacteroidetes (F/B) ratio	893:917	Bacteroidetes (F/B) ratio	893:917	The HFD lowered bacterial diversity, increased the Firmicutes: Bacteroidetes (F/B) ratio and altered composition.
33498853	9	55	theme	F/B	1132:1134	arg1	ratio					1136:1140	the F/B ratio	1128:1140	the F/B ratio	1128:1140	Kale supplementation lowered the F/B ratio, increased both alpha and beta diversity and reduced class Bacilli and Erysipelotrichi but had no effect on Clostridia.
33498853	2	56	theme	functional	229:238	arg1	acephala					155:162	acephala	155:162	acephala	155:162	acephala) is a vegetable common in most cultures but is less studied as a functional food compared to other cruciferous vegetables, such as broccoli.
33498853	2	56	theme	functional	229:238	arg1	food					240:243	a functional food	227:243	a functional food compared to other cruciferous vegetables, such as broccoli	227:302	acephala) is a vegetable common in most cultures but is less studied as a functional food compared to other cruciferous vegetables, such as broccoli.
33498853	0	57	with	Composition	57:67	arg1	Obesity					117:123	Diet-Induced Obesity	104:123	Diet-Induced Obesity	104:123	Kale Attenuates Inflammation and Modulates Gut Microbial Composition and Function in C57BL/6J Mice with Diet-Induced Obesity.
33498853	9	58	theme	Kale	1099:1102	arg1	supplementation					1104:1118	Kale supplementation	1099:1118	Kale supplementation	1099:1118	Kale supplementation lowered the F/B ratio, increased both alpha and beta diversity and reduced class Bacilli and Erysipelotrichi but had no effect on Clostridia.
33498853	9	59	contain	had	1233:1235	arg2	effect					1240:1245	no effect	1237:1245	no effect	1237:1245	Kale supplementation lowered the F/B ratio, increased both alpha and beta diversity and reduced class Bacilli and Erysipelotrichi but had no effect on Clostridia.
33498853	9	59	contain	had	1233:1235	arg1	supplementation					1104:1118	Kale supplementation	1099:1118	Kale supplementation	1099:1118	Kale supplementation lowered the F/B ratio, increased both alpha and beta diversity and reduced class Bacilli and Erysipelotrichi but had no effect on Clostridia.
33498853	12	60	theme	polysaccharide	1548:1561	arg1	enzymes					1573:1579	plant polysaccharide digesting enzymes	1542:1579	plant polysaccharide digesting enzymes	1542:1579	This species produces plant polysaccharide digesting enzymes.
33498853	5	61	theme	HFD-induced	723:733	arg1	inflammation					735:746	HFD-induced inflammation	723:746	HFD-induced inflammation	723:746	Like other cruciferous vegetables, kale attenuated HFD-induced inflammation.
33498853	6	62	theme	microbiota	806:815	arg1	composition					817:827	cecal microbiota composition	800:827	cecal microbiota composition	800:827	In addition, kale modulated HFD-induced changes in cecal microbiota composition.
33498853	4	63	theme	community	646:654	arg1	potential					611:619	the functional potential	596:619	the functional potential of the altered bacterial community	596:654	We particularly explored its role in metabolic parameters, gut bacterial composition and diversity using 16S rRNA sequencing, systematically compared changes under each phylum and predicted the functional potential of the altered bacterial community using PICRUSt2.
33498853	7	64	theme	Bacteroidetes	893:905	arg1	Firmicutes					881:890	the Firmicutes	877:890	the Firmicutes: Bacteroidetes (F/B) ratio and altered composition	877:941	The HFD lowered bacterial diversity, increased the Firmicutes: Bacteroidetes (F/B) ratio and altered composition.
33498853	7	64	theme	Bacteroidetes	893:905	arg1	ratio					913:917	Bacteroidetes (F/B) ratio	893:917	Bacteroidetes (F/B) ratio	893:917	The HFD lowered bacterial diversity, increased the Firmicutes: Bacteroidetes (F/B) ratio and altered composition.
33498853	8	65	dep	Firmicutes	1064:1073	arg1	Bacilli					1089:1095	class Bacilli	1083:1095	mainly class Bacilli	1076:1095	Specifically, it lowered Actinobacteria and Bacteroidetes (Bacteroidia, Rikenellaceae and Prevotellaceae) but increased Firmicutes (mainly class Bacilli).
33498853	4	66	theme	metabolic	443:451	arg1	parameters					453:462	metabolic parameters	443:462	metabolic parameters	443:462	We particularly explored its role in metabolic parameters, gut bacterial composition and diversity using 16S rRNA sequencing, systematically compared changes under each phylum and predicted the functional potential of the altered bacterial community using PICRUSt2.
33498853	4	67	from	diversity	495:503	arg1	parameters					453:462	metabolic parameters	443:462	metabolic parameters	443:462	We particularly explored its role in metabolic parameters, gut bacterial composition and diversity using 16S rRNA sequencing, systematically compared changes under each phylum and predicted the functional potential of the altered bacterial community using PICRUSt2.
33498853	9	68	theme	alpha	1158:1162	arg1	diversity					1173:1181	both alpha and beta diversity	1153:1181	both alpha and beta diversity	1153:1181	Kale supplementation lowered the F/B ratio, increased both alpha and beta diversity and reduced class Bacilli and Erysipelotrichi but had no effect on Clostridia.
33498853	4	69	theme	bacterial	636:644	arg1	community					646:654	the altered bacterial community	624:654	the altered bacterial community	624:654	We particularly explored its role in metabolic parameters, gut bacterial composition and diversity using 16S rRNA sequencing, systematically compared changes under each phylum and predicted the functional potential of the altered bacterial community using PICRUSt2.
33498853	14	70	dep	microbiota	1829:1838	arg1	phenotype					1868:1876	phenotype	1868:1876	phenotype	1868:1876	Our findings provide evidence that kale is a functional food that modulates the microbiota and changes in inflammation phenotype.
31941510	2	0	theme	glucose	555:561	arg1	tests					573:577	glucose tolerance tests	555:577	glucose tolerance tests (GTTs)	555:584	The present study was designed to reveal relationships among BA metabolism, glucose tolerance, and cecal organic acids in rats fed a high-fat and high-sucrose diet (HFS) by using multivariate and multiple regression analyses in two types of glucose tolerance tests (GTTs).
31941510	2	0	theme	glucose	555:561	arg1	GTTs					580:583	GTTs	580:583	GTTs	580:583	The present study was designed to reveal relationships among BA metabolism, glucose tolerance, and cecal organic acids in rats fed a high-fat and high-sucrose diet (HFS) by using multivariate and multiple regression analyses in two types of glucose tolerance tests (GTTs).
31941510	11	1	theme	cecal	1789:1793	arg1	contents					1795:1802	the cecal contents	1785:1802	the cecal contents of the HFS-fed rats	1785:1822	In contrast, a decrease in the concentration of organic acids was observed in the cecal contents of the HFS-fed rats.
31941510	1	2	theme	bile	247:250	arg1	BA					258:259	BA	258:259	BA	258:259	BACKGROUND Previously, we found a significant relationship in a rat study between energy intake and bile acid (BA) metabolism especially 12α-hydroxylated (12αOH) BAs.
31941510	1	2	theme	bile	247:250	arg1	acid					252:255	bile acid	247:255	bile acid (BA) metabolism	247:271	BACKGROUND Previously, we found a significant relationship in a rat study between energy intake and bile acid (BA) metabolism especially 12α-hydroxylated (12αOH) BAs.
31941510	12	3	theme	12αOH	1917:1921	arg1	BA					1923:1924	aortic 12αOH BA	1910:1924	aortic 12αOH BA	1910:1924	Multiple linear regression analysis in the IPGTT revealed that the concentrations of aortic 12αOH BA and cecal acetic acid were the predictors of insulin secretion.
31941510	14	4	from	GTTs	2304:2307	arg1	rats					2312:2315	rats	2312:2315	rats	2312:2315	CONCLUSION The distribution analysis of BA compositions accompanied by GTTs revealed a close relationship between 12αOH BA metabolism and insulin secretion in GTTs in rats.
31941510	7	5	theme	positive	1071:1078	arg1	correlation					1080:1090	A positive correlation	1069:1090	A positive correlation of aortic 12αOH BA concentration	1069:1123	RESULTS A positive correlation of aortic 12αOH BA concentration was observed with energy intake and visceral adipose tissue weight.
31941510	2	6	theme	tests	573:577	arg1	types					546:550	two types	542:550	two types of glucose tolerance tests (GTTs)	542:584	The present study was designed to reveal relationships among BA metabolism, glucose tolerance, and cecal organic acids in rats fed a high-fat and high-sucrose diet (HFS) by using multivariate and multiple regression analyses in two types of glucose tolerance tests (GTTs).
31941510	13	7	theme	insulin	2089:2095	arg1	concentration					2097:2109	insulin concentration	2089:2109	insulin concentration of peripheral blood in the IPGTT	2089:2142	Moreover, there was a positive correlation between concentration of portal 12αOH BAs and change in insulin concentration of peripheral blood in the IPGTT.
31941510	1	8	theme	significant	181:191	arg1	relationship					193:204	a significant relationship	179:204	a significant relationship in a rat study between energy intake and bile acid (BA) metabolism especially 12α-hydroxylated (12αOH) BAs	179:311	BACKGROUND Previously, we found a significant relationship in a rat study between energy intake and bile acid (BA) metabolism especially 12α-hydroxylated (12αOH) BAs.
31941510	6	9	theme	acid	896:899	arg1	concentrations					901:914	Organic acid concentrations	888:914	Organic acid concentrations in cecal contents	888:932	Organic acid concentrations in cecal contents were analyzed by using ultra high-performance liquid chromatography with post-column pH buffered electric conductivity method.
31941510	14	10	from	secretion	2291:2299	arg1	GTTs					2304:2307	GTTs	2304:2307	GTTs in rats	2304:2315	CONCLUSION The distribution analysis of BA compositions accompanied by GTTs revealed a close relationship between 12αOH BA metabolism and insulin secretion in GTTs in rats.
31941510	8	11	theme	12αOH	1222:1226	arg1	BAs					1228:1230	12αOH BAs	1222:1230	12αOH BAs	1222:1230	We found that an increase of 12αOH BAs in enterohepatic circulation, intestinal contents and feces in the HFS-fed rats compared to those in control rats regardless of no significant increase of total BA concentration in the feces in the test period.
31941510	0	12	theme	high-fat	115:122	arg1	diet					141:144	a high-fat and high-sucrose diet	113:144	a high-fat and high-sucrose diet	113:144	Correlation between 12α-hydroxylated bile acids and insulin secretion during glucose tolerance tests in rats fed a high-fat and high-sucrose diet.
31941510	8	13	from	increase	1375:1382	arg1	feces					1417:1421	the feces	1413:1421	the feces in the test period	1413:1440	We found that an increase of 12αOH BAs in enterohepatic circulation, intestinal contents and feces in the HFS-fed rats compared to those in control rats regardless of no significant increase of total BA concentration in the feces in the test period.
31941510	12	14	theme	secretion	1979:1987	arg1	concentrations					1892:1905	the concentrations	1888:1905	the concentrations of aortic 12αOH BA and cecal acetic acid	1888:1946	Multiple linear regression analysis in the IPGTT revealed that the concentrations of aortic 12αOH BA and cecal acetic acid were the predictors of insulin secretion.
31941510	12	14	theme	secretion	1979:1987	arg1	predictors					1957:1966	the predictors	1953:1966	the predictors of insulin secretion	1953:1987	Multiple linear regression analysis in the IPGTT revealed that the concentrations of aortic 12αOH BA and cecal acetic acid were the predictors of insulin secretion.
31941510	8	15	theme	BA	1393:1394	arg1	concentration					1396:1408	total BA concentration	1387:1408	total BA concentration in the feces in the test period	1387:1440	We found that an increase of 12αOH BAs in enterohepatic circulation, intestinal contents and feces in the HFS-fed rats compared to those in control rats regardless of no significant increase of total BA concentration in the feces in the test period.
31941510	11	16	theme	HFS-fed	1811:1817	arg1	rats					1819:1822	the HFS-fed rats	1807:1822	the HFS-fed rats	1807:1822	In contrast, a decrease in the concentration of organic acids was observed in the cecal contents of the HFS-fed rats.
31941510	0	17	theme	high-sucrose	128:139	arg1	diet					141:144	a high-fat and high-sucrose diet	113:144	a high-fat and high-sucrose diet	113:144	Correlation between 12α-hydroxylated bile acids and insulin secretion during glucose tolerance tests in rats fed a high-fat and high-sucrose diet.
31941510	8	18	from	increase	1210:1217	arg1	rats					1307:1310	the HFS-fed rats	1295:1310	the HFS-fed rats	1295:1310	We found that an increase of 12αOH BAs in enterohepatic circulation, intestinal contents and feces in the HFS-fed rats compared to those in control rats regardless of no significant increase of total BA concentration in the feces in the test period.
31941510	8	18	from	increase	1210:1217	arg1	feces					1286:1290	feces	1286:1290	feces	1286:1290	We found that an increase of 12αOH BAs in enterohepatic circulation, intestinal contents and feces in the HFS-fed rats compared to those in control rats regardless of no significant increase of total BA concentration in the feces in the test period.
31941510	8	18	from	increase	1210:1217	arg1	circulation					1249:1259	enterohepatic circulation	1235:1259	enterohepatic circulation	1235:1259	We found that an increase of 12αOH BAs in enterohepatic circulation, intestinal contents and feces in the HFS-fed rats compared to those in control rats regardless of no significant increase of total BA concentration in the feces in the test period.
31941510	8	18	from	increase	1210:1217	arg1	contents					1273:1280	intestinal contents	1262:1280	intestinal contents	1262:1280	We found that an increase of 12αOH BAs in enterohepatic circulation, intestinal contents and feces in the HFS-fed rats compared to those in control rats regardless of no significant increase of total BA concentration in the feces in the test period.
31941510	1	19	theme	rat	211:213	arg1	study					215:219	a rat study	209:219	a rat study	209:219	BACKGROUND Previously, we found a significant relationship in a rat study between energy intake and bile acid (BA) metabolism especially 12α-hydroxylated (12αOH) BAs.
31941510	7	20	dep	RESULTS	1061:1067	arg1	observed					1129:1136	observed	1129:1136	was observed with energy intake and visceral adipose tissue weight	1125:1190	RESULTS A positive correlation of aortic 12αOH BA concentration was observed with energy intake and visceral adipose tissue weight.
31941510	1	21	from	relationship	193:204	arg1	study					215:219	a rat study	209:219	a rat study	209:219	BACKGROUND Previously, we found a significant relationship in a rat study between energy intake and bile acid (BA) metabolism especially 12α-hydroxylated (12αOH) BAs.
31941510	2	22	theme	regression	519:528	arg1	analyses					530:537	multivariate and multiple regression analyses	493:537	analyses	530:537	The present study was designed to reveal relationships among BA metabolism, glucose tolerance, and cecal organic acids in rats fed a high-fat and high-sucrose diet (HFS) by using multivariate and multiple regression analyses in two types of glucose tolerance tests (GTTs).
31941510	8	23	theme	intestinal	1262:1271	arg1	contents					1273:1280	intestinal contents	1262:1280	intestinal contents	1262:1280	We found that an increase of 12αOH BAs in enterohepatic circulation, intestinal contents and feces in the HFS-fed rats compared to those in control rats regardless of no significant increase of total BA concentration in the feces in the test period.
31941510	4	24	theme	tolerance	677:685	arg1	OGTT					693:696	OGTT	693:696	OGTT	693:696	Oral glucose tolerance test (OGTT) and intraperitoneal glucose tolerance test (IPGTT) were performed at week 9 and 11, respectively.
31941510	4	24	theme	tolerance	677:685	arg1	test					687:690	Oral glucose tolerance test	664:690	Oral glucose tolerance test (OGTT)	664:697	Oral glucose tolerance test (OGTT) and intraperitoneal glucose tolerance test (IPGTT) were performed at week 9 and 11, respectively.
31941510	9	25	theme	12αOH	1449:1453	arg1	concentration					1458:1470	Fecal 12αOH BA concentration	1443:1470	Fecal 12αOH BA concentration	1443:1470	Fecal 12αOH BA concentration was positively correlated with maximal insulin level in OGTT and area under curve of insulin in IPGTT.
31941510	9	26	from	level	1519:1523	arg1	OGTT					1528:1531	OGTT	1528:1531	OGTT	1528:1531	Fecal 12αOH BA concentration was positively correlated with maximal insulin level in OGTT and area under curve of insulin in IPGTT.
31941510	2	27	theme	high-sucrose	460:471	arg1	HFS					479:481	HFS	479:481	HFS	479:481	The present study was designed to reveal relationships among BA metabolism, glucose tolerance, and cecal organic acids in rats fed a high-fat and high-sucrose diet (HFS) by using multivariate and multiple regression analyses in two types of glucose tolerance tests (GTTs).
31941510	2	27	theme	high-sucrose	460:471	arg1	diet					473:476	a high-fat and high-sucrose diet	445:476	diet	473:476	The present study was designed to reveal relationships among BA metabolism, glucose tolerance, and cecal organic acids in rats fed a high-fat and high-sucrose diet (HFS) by using multivariate and multiple regression analyses in two types of glucose tolerance tests (GTTs).
31941510	11	28	theme	rats	1819:1822	arg1	contents					1795:1802	the cecal contents	1785:1802	the cecal contents of the HFS-fed rats	1785:1822	In contrast, a decrease in the concentration of organic acids was observed in the cecal contents of the HFS-fed rats.
31941510	13	29	theme	BAs	2071:2073	arg1	concentration					2041:2053	concentration	2041:2053	concentration of portal 12αOH BAs	2041:2073	Moreover, there was a positive correlation between concentration of portal 12αOH BAs and change in insulin concentration of peripheral blood in the IPGTT.
31941510	13	29	theme	BAs	2071:2073	arg1	change					2079:2084	change	2079:2084	change in insulin concentration of peripheral blood in the IPGTT	2079:2142	Moreover, there was a positive correlation between concentration of portal 12αOH BAs and change in insulin concentration of peripheral blood in the IPGTT.
31941510	12	30	from	analysis	1852:1859	arg1	IPGTT					1868:1872	the IPGTT	1864:1872	the IPGTT	1864:1872	Multiple linear regression analysis in the IPGTT revealed that the concentrations of aortic 12αOH BA and cecal acetic acid were the predictors of insulin secretion.
31941510	2	31	theme	high-fat	447:454	arg1	HFS					479:481	HFS	479:481	HFS	479:481	The present study was designed to reveal relationships among BA metabolism, glucose tolerance, and cecal organic acids in rats fed a high-fat and high-sucrose diet (HFS) by using multivariate and multiple regression analyses in two types of glucose tolerance tests (GTTs).
31941510	2	31	theme	high-fat	447:454	arg1	diet					473:476	a high-fat and high-sucrose diet	445:476	diet	473:476	The present study was designed to reveal relationships among BA metabolism, glucose tolerance, and cecal organic acids in rats fed a high-fat and high-sucrose diet (HFS) by using multivariate and multiple regression analyses in two types of glucose tolerance tests (GTTs).
31941510	12	32	theme	insulin	1971:1977	arg1	secretion					1979:1987	insulin secretion	1971:1987	insulin secretion	1971:1987	Multiple linear regression analysis in the IPGTT revealed that the concentrations of aortic 12αOH BA and cecal acetic acid were the predictors of insulin secretion.
31941510	0	33	theme	tolerance	85:93	arg1	tests					95:99	glucose tolerance tests	77:99	glucose tolerance tests in rats	77:107	Correlation between 12α-hydroxylated bile acids and insulin secretion during glucose tolerance tests in rats fed a high-fat and high-sucrose diet.
31941510	12	34	theme	Multiple	1825:1832	arg1	analysis					1852:1859	Multiple linear regression analysis	1825:1859	Multiple linear regression analysis in the IPGTT	1825:1872	Multiple linear regression analysis in the IPGTT revealed that the concentrations of aortic 12αOH BA and cecal acetic acid were the predictors of insulin secretion.
31941510	8	35	theme	HFS-fed	1299:1305	arg1	rats					1307:1310	the HFS-fed rats	1295:1310	the HFS-fed rats	1295:1310	We found that an increase of 12αOH BAs in enterohepatic circulation, intestinal contents and feces in the HFS-fed rats compared to those in control rats regardless of no significant increase of total BA concentration in the feces in the test period.
31941510	1	36	dep	BACKGROUND	147:156	arg1	found					173:177	found	173:177	found a significant relationship in a rat study between energy intake and bile acid (BA) metabolism especially 12α-hydroxylated (12αOH) BAs	173:311	BACKGROUND Previously, we found a significant relationship in a rat study between energy intake and bile acid (BA) metabolism especially 12α-hydroxylated (12αOH) BAs.
31941510	7	37	theme	adipose	1170:1176	arg1	weight					1185:1190	visceral adipose tissue weight	1161:1190	visceral adipose tissue weight	1161:1190	RESULTS A positive correlation of aortic 12αOH BA concentration was observed with energy intake and visceral adipose tissue weight.
31941510	12	38	theme	regression	1841:1850	arg1	analysis					1852:1859	Multiple linear regression analysis	1825:1859	Multiple linear regression analysis in the IPGTT	1825:1872	Multiple linear regression analysis in the IPGTT revealed that the concentrations of aortic 12αOH BA and cecal acetic acid were the predictors of insulin secretion.
31941510	5	39	theme	ultra	824:828	arg1	spectrometry					874:885	ultra high-performance liquid chromatography-mass spectrometry	824:885	ultra high-performance liquid chromatography-mass spectrometry	824:885	BAs were analyzed by using ultra high-performance liquid chromatography-mass spectrometry.
31941510	14	40	dep	CONCLUSION	2145:2154	arg1	revealed					2221:2228	revealed	2221:2228	revealed a close relationship between 12αOH BA metabolism and insulin secretion in GTTs in rats	2221:2315	CONCLUSION The distribution analysis of BA compositions accompanied by GTTs revealed a close relationship between 12αOH BA metabolism and insulin secretion in GTTs in rats.
31941510	2	41	from	acids	427:431	arg1	rats					436:439	rats	436:439	rats fed a high-fat and high-sucrose diet (HFS) by using multivariate and multiple regression analyses in two types of glucose tolerance tests (GTTs)	436:584	The present study was designed to reveal relationships among BA metabolism, glucose tolerance, and cecal organic acids in rats fed a high-fat and high-sucrose diet (HFS) by using multivariate and multiple regression analyses in two types of glucose tolerance tests (GTTs).
31941510	10	42	theme	aortic	1616:1621	arg1	concentration					1633:1645	aortic 12αOH BAs concentration	1616:1645	aortic 12αOH BAs concentration	1616:1645	There was a positive correlation between aortic 12αOH BAs concentration and changes in plasma glucose level in both OGTT and IPGTT.
31941510	2	43	theme	BA	375:376	arg1	metabolism					378:387	BA metabolism	375:387	BA metabolism	375:387	The present study was designed to reveal relationships among BA metabolism, glucose tolerance, and cecal organic acids in rats fed a high-fat and high-sucrose diet (HFS) by using multivariate and multiple regression analyses in two types of glucose tolerance tests (GTTs).
31941510	8	44	from	feces	1417:1421	arg1	increase					1375:1382	no significant increase	1360:1382	no significant increase of total BA concentration in the feces in the test period	1360:1440	We found that an increase of 12αOH BAs in enterohepatic circulation, intestinal contents and feces in the HFS-fed rats compared to those in control rats regardless of no significant increase of total BA concentration in the feces in the test period.
31941510	8	44	from	feces	1417:1421	arg1	period					1435:1440	the test period	1426:1440	the test period	1426:1440	We found that an increase of 12αOH BAs in enterohepatic circulation, intestinal contents and feces in the HFS-fed rats compared to those in control rats regardless of no significant increase of total BA concentration in the feces in the test period.
31941510	9	45	theme	insulin	1511:1517	arg1	level					1519:1523	maximal insulin level	1503:1523	maximal insulin level in OGTT	1503:1531	Fecal 12αOH BA concentration was positively correlated with maximal insulin level in OGTT and area under curve of insulin in IPGTT.
31941510	6	46	theme	buffered	1022:1029	arg1	method					1053:1058	post-column pH buffered electric conductivity method	1007:1058	post-column pH buffered electric conductivity method	1007:1058	Organic acid concentrations in cecal contents were analyzed by using ultra high-performance liquid chromatography with post-column pH buffered electric conductivity method.
31941510	2	47	theme	organic	419:425	arg1	acids					427:431	cecal organic acids	413:431	cecal organic acids	413:431	The present study was designed to reveal relationships among BA metabolism, glucose tolerance, and cecal organic acids in rats fed a high-fat and high-sucrose diet (HFS) by using multivariate and multiple regression analyses in two types of glucose tolerance tests (GTTs).
31941510	4	48	theme	Oral	664:667	arg1	OGTT					693:696	OGTT	693:696	OGTT	693:696	Oral glucose tolerance test (OGTT) and intraperitoneal glucose tolerance test (IPGTT) were performed at week 9 and 11, respectively.
31941510	4	48	theme	Oral	664:667	arg1	test					687:690	Oral glucose tolerance test	664:690	Oral glucose tolerance test (OGTT)	664:697	Oral glucose tolerance test (OGTT) and intraperitoneal glucose tolerance test (IPGTT) were performed at week 9 and 11, respectively.
31941510	14	49	theme	BA	2185:2186	arg1	compositions					2188:2199	BA compositions	2185:2199	BA compositions accompanied by GTTs	2185:2219	CONCLUSION The distribution analysis of BA compositions accompanied by GTTs revealed a close relationship between 12αOH BA metabolism and insulin secretion in GTTs in rats.
31941510	7	50	theme	energy	1143:1148	arg1	intake					1150:1155	energy intake	1143:1155	energy intake	1143:1155	RESULTS A positive correlation of aortic 12αOH BA concentration was observed with energy intake and visceral adipose tissue weight.
31941510	5	51	theme	liquid	847:852	arg1	spectrometry					874:885	ultra high-performance liquid chromatography-mass spectrometry	824:885	ultra high-performance liquid chromatography-mass spectrometry	824:885	BAs were analyzed by using ultra high-performance liquid chromatography-mass spectrometry.
31941510	9	52	from	curve	1548:1552	arg1	IPGTT					1568:1572	IPGTT	1568:1572	IPGTT	1568:1572	Fecal 12αOH BA concentration was positively correlated with maximal insulin level in OGTT and area under curve of insulin in IPGTT.
31941510	6	53	theme	post-column	1007:1017	arg1	method					1053:1058	post-column pH buffered electric conductivity method	1007:1058	post-column pH buffered electric conductivity method	1007:1058	Organic acid concentrations in cecal contents were analyzed by using ultra high-performance liquid chromatography with post-column pH buffered electric conductivity method.
31941510	8	54	theme	control	1333:1339	arg1	rats					1341:1344	control rats	1333:1344	control rats	1333:1344	We found that an increase of 12αOH BAs in enterohepatic circulation, intestinal contents and feces in the HFS-fed rats compared to those in control rats regardless of no significant increase of total BA concentration in the feces in the test period.
31941510	10	55	theme	12αOH	1623:1627	arg1	concentration					1633:1645	aortic 12αOH BAs concentration	1616:1645	aortic 12αOH BAs concentration	1616:1645	There was a positive correlation between aortic 12αOH BAs concentration and changes in plasma glucose level in both OGTT and IPGTT.
31941510	0	56	theme	12α-hydroxylated	20:35	arg1	acids					42:46	12α-hydroxylated bile acids	20:46	12α-hydroxylated bile acids	20:46	Correlation between 12α-hydroxylated bile acids and insulin secretion during glucose tolerance tests in rats fed a high-fat and high-sucrose diet.
31941510	9	57	from	area	1537:1540	arg1	OGTT					1528:1531	OGTT	1528:1531	OGTT	1528:1531	Fecal 12αOH BA concentration was positively correlated with maximal insulin level in OGTT and area under curve of insulin in IPGTT.
31941510	6	58	from	concentrations	901:914	arg1	contents					925:932	cecal contents	919:932	cecal contents	919:932	Organic acid concentrations in cecal contents were analyzed by using ultra high-performance liquid chromatography with post-column pH buffered electric conductivity method.
31941510	3	59	theme	Male	595:598	arg1	rats					612:615	Male WKAH/HkmSlc rats	595:615	Male WKAH/HkmSlc rats	595:615	METHODS Male WKAH/HkmSlc rats were fed with a control or a HFS for 13 weeks.
31941510	7	60	theme	concentration	1111:1123	arg1	correlation					1080:1090	A positive correlation	1069:1090	A positive correlation of aortic 12αOH BA concentration	1069:1123	RESULTS A positive correlation of aortic 12αOH BA concentration was observed with energy intake and visceral adipose tissue weight.
31941510	1	61	theme	12α-hydroxylated	284:299	arg1	BAs					309:311	especially 12α-hydroxylated (12αOH) BAs	273:311	especially 12α-hydroxylated (12αOH) BAs	273:311	BACKGROUND Previously, we found a significant relationship in a rat study between energy intake and bile acid (BA) metabolism especially 12α-hydroxylated (12αOH) BAs.
31941510	6	62	theme	high-performance	963:978	arg1	chromatography					987:1000	ultra high-performance liquid chromatography	957:1000	ultra high-performance liquid chromatography	957:1000	Organic acid concentrations in cecal contents were analyzed by using ultra high-performance liquid chromatography with post-column pH buffered electric conductivity method.
31941510	2	63	theme	present	318:324	arg1	study					326:330	The present study	314:330	The present study	314:330	The present study was designed to reveal relationships among BA metabolism, glucose tolerance, and cecal organic acids in rats fed a high-fat and high-sucrose diet (HFS) by using multivariate and multiple regression analyses in two types of glucose tolerance tests (GTTs).
31941510	7	64	theme	12αOH	1102:1106	arg1	concentration					1111:1123	aortic 12αOH BA concentration	1095:1123	aortic 12αOH BA concentration	1095:1123	RESULTS A positive correlation of aortic 12αOH BA concentration was observed with energy intake and visceral adipose tissue weight.
31941510	13	65	theme	blood	2125:2129	arg1	concentration					2097:2109	insulin concentration	2089:2109	insulin concentration of peripheral blood in the IPGTT	2089:2142	Moreover, there was a positive correlation between concentration of portal 12αOH BAs and change in insulin concentration of peripheral blood in the IPGTT.
31941510	13	66	from	change	2079:2084	arg1	concentration					2097:2109	insulin concentration	2089:2109	insulin concentration of peripheral blood in the IPGTT	2089:2142	Moreover, there was a positive correlation between concentration of portal 12αOH BAs and change in insulin concentration of peripheral blood in the IPGTT.
31941510	4	67	theme	intraperitoneal	703:717	arg1	IPGTT					743:747	IPGTT	743:747	IPGTT	743:747	Oral glucose tolerance test (OGTT) and intraperitoneal glucose tolerance test (IPGTT) were performed at week 9 and 11, respectively.
31941510	4	67	theme	intraperitoneal	703:717	arg1	test					737:740	intraperitoneal glucose tolerance test	703:740	intraperitoneal glucose tolerance test (IPGTT)	703:748	Oral glucose tolerance test (OGTT) and intraperitoneal glucose tolerance test (IPGTT) were performed at week 9 and 11, respectively.
31941510	11	68	theme	acids	1763:1767	arg1	concentration					1738:1750	the concentration	1734:1750	the concentration of organic acids	1734:1767	In contrast, a decrease in the concentration of organic acids was observed in the cecal contents of the HFS-fed rats.
31941510	8	69	theme	total	1387:1391	arg1	concentration					1396:1408	total BA concentration	1387:1408	total BA concentration in the feces in the test period	1387:1440	We found that an increase of 12αOH BAs in enterohepatic circulation, intestinal contents and feces in the HFS-fed rats compared to those in control rats regardless of no significant increase of total BA concentration in the feces in the test period.
31941510	3	70	dep	METHODS	587:593	arg1	fed					622:624	fed	622:624	were fed with a control or a HFS for 13 weeks	617:661	METHODS Male WKAH/HkmSlc rats were fed with a control or a HFS for 13 weeks.
31941510	12	71	theme	cecal	1930:1934	arg1	acid					1943:1946	cecal acetic acid	1930:1946	cecal acetic acid	1930:1946	Multiple linear regression analysis in the IPGTT revealed that the concentrations of aortic 12αOH BA and cecal acetic acid were the predictors of insulin secretion.
31941510	2	72	from	tolerance	398:406	arg1	rats					436:439	rats	436:439	rats fed a high-fat and high-sucrose diet (HFS) by using multivariate and multiple regression analyses in two types of glucose tolerance tests (GTTs)	436:584	The present study was designed to reveal relationships among BA metabolism, glucose tolerance, and cecal organic acids in rats fed a high-fat and high-sucrose diet (HFS) by using multivariate and multiple regression analyses in two types of glucose tolerance tests (GTTs).
31941510	8	73	from	concentration	1396:1408	arg1	feces					1417:1421	the feces	1413:1421	the feces in the test period	1413:1440	We found that an increase of 12αOH BAs in enterohepatic circulation, intestinal contents and feces in the HFS-fed rats compared to those in control rats regardless of no significant increase of total BA concentration in the feces in the test period.
31941510	10	74	theme	plasma	1662:1667	arg1	level					1677:1681	plasma glucose level	1662:1681	plasma glucose level	1662:1681	There was a positive correlation between aortic 12αOH BAs concentration and changes in plasma glucose level in both OGTT and IPGTT.
31941510	13	75	theme	portal	2058:2063	arg1	BAs					2071:2073	portal 12αOH BAs	2058:2073	portal 12αOH BAs	2058:2073	Moreover, there was a positive correlation between concentration of portal 12αOH BAs and change in insulin concentration of peripheral blood in the IPGTT.
31941510	12	76	theme	acid	1943:1946	arg1	concentrations					1892:1905	the concentrations	1888:1905	the concentrations of aortic 12αOH BA and cecal acetic acid	1888:1946	Multiple linear regression analysis in the IPGTT revealed that the concentrations of aortic 12αOH BA and cecal acetic acid were the predictors of insulin secretion.
31941510	12	76	theme	acid	1943:1946	arg1	predictors					1957:1966	the predictors	1953:1966	the predictors of insulin secretion	1953:1987	Multiple linear regression analysis in the IPGTT revealed that the concentrations of aortic 12αOH BA and cecal acetic acid were the predictors of insulin secretion.
31941510	11	77	from	decrease	1722:1729	arg1	concentration					1738:1750	the concentration	1734:1750	the concentration of organic acids	1734:1767	In contrast, a decrease in the concentration of organic acids was observed in the cecal contents of the HFS-fed rats.
31941510	10	78	from	concentration	1633:1645	arg1	OGTT					1691:1694	OGTT	1691:1694	OGTT	1691:1694	There was a positive correlation between aortic 12αOH BAs concentration and changes in plasma glucose level in both OGTT and IPGTT.
31941510	10	78	from	concentration	1633:1645	arg1	IPGTT					1700:1704	IPGTT	1700:1704	IPGTT	1700:1704	There was a positive correlation between aortic 12αOH BAs concentration and changes in plasma glucose level in both OGTT and IPGTT.
31941510	10	78	from	concentration	1633:1645	arg1	level					1677:1681	plasma glucose level	1662:1681	plasma glucose level	1662:1681	There was a positive correlation between aortic 12αOH BAs concentration and changes in plasma glucose level in both OGTT and IPGTT.
31941510	6	79	theme	conductivity	1040:1051	arg1	method					1053:1058	post-column pH buffered electric conductivity method	1007:1058	post-column pH buffered electric conductivity method	1007:1058	Organic acid concentrations in cecal contents were analyzed by using ultra high-performance liquid chromatography with post-column pH buffered electric conductivity method.
31941510	13	80	from	concentration	2041:2053	arg1	concentration					2097:2109	insulin concentration	2089:2109	insulin concentration of peripheral blood in the IPGTT	2089:2142	Moreover, there was a positive correlation between concentration of portal 12αOH BAs and change in insulin concentration of peripheral blood in the IPGTT.
31941510	1	81	theme	acid	252:255	arg1	metabolism					262:271	bile acid (BA) metabolism	247:271	bile acid (BA) metabolism	247:271	BACKGROUND Previously, we found a significant relationship in a rat study between energy intake and bile acid (BA) metabolism especially 12α-hydroxylated (12αOH) BAs.
31941510	12	82	theme	aortic	1910:1915	arg1	BA					1923:1924	aortic 12αOH BA	1910:1924	aortic 12αOH BA	1910:1924	Multiple linear regression analysis in the IPGTT revealed that the concentrations of aortic 12αOH BA and cecal acetic acid were the predictors of insulin secretion.
31941510	10	83	from	changes	1651:1657	arg1	OGTT					1691:1694	OGTT	1691:1694	OGTT	1691:1694	There was a positive correlation between aortic 12αOH BAs concentration and changes in plasma glucose level in both OGTT and IPGTT.
31941510	10	83	from	changes	1651:1657	arg1	IPGTT					1700:1704	IPGTT	1700:1704	IPGTT	1700:1704	There was a positive correlation between aortic 12αOH BAs concentration and changes in plasma glucose level in both OGTT and IPGTT.
31941510	10	83	from	changes	1651:1657	arg1	level					1677:1681	plasma glucose level	1662:1681	plasma glucose level	1662:1681	There was a positive correlation between aortic 12αOH BAs concentration and changes in plasma glucose level in both OGTT and IPGTT.
31941510	0	84	theme	insulin	52:58	arg1	secretion					60:68	insulin secretion	52:68	insulin secretion during glucose tolerance tests in rats	52:107	Correlation between 12α-hydroxylated bile acids and insulin secretion during glucose tolerance tests in rats fed a high-fat and high-sucrose diet.
31941510	14	85	theme	insulin	2283:2289	arg1	secretion					2291:2299	insulin secretion	2283:2299	insulin secretion	2283:2299	CONCLUSION The distribution analysis of BA compositions accompanied by GTTs revealed a close relationship between 12αOH BA metabolism and insulin secretion in GTTs in rats.
31941510	2	86	theme	tolerance	563:571	arg1	tests					573:577	glucose tolerance tests	555:577	glucose tolerance tests (GTTs)	555:584	The present study was designed to reveal relationships among BA metabolism, glucose tolerance, and cecal organic acids in rats fed a high-fat and high-sucrose diet (HFS) by using multivariate and multiple regression analyses in two types of glucose tolerance tests (GTTs).
31941510	2	86	theme	tolerance	563:571	arg1	GTTs					580:583	GTTs	580:583	GTTs	580:583	The present study was designed to reveal relationships among BA metabolism, glucose tolerance, and cecal organic acids in rats fed a high-fat and high-sucrose diet (HFS) by using multivariate and multiple regression analyses in two types of glucose tolerance tests (GTTs).
31941510	14	87	from	metabolism	2268:2277	arg1	GTTs					2304:2307	GTTs	2304:2307	GTTs in rats	2304:2315	CONCLUSION The distribution analysis of BA compositions accompanied by GTTs revealed a close relationship between 12αOH BA metabolism and insulin secretion in GTTs in rats.
31941510	6	88	theme	Organic	888:894	arg1	concentrations					901:914	Organic acid concentrations	888:914	Organic acid concentrations in cecal contents	888:932	Organic acid concentrations in cecal contents were analyzed by using ultra high-performance liquid chromatography with post-column pH buffered electric conductivity method.
31941510	2	89	theme	multivariate	493:504	arg1	analyses					530:537	multivariate and multiple regression analyses	493:537	analyses	530:537	The present study was designed to reveal relationships among BA metabolism, glucose tolerance, and cecal organic acids in rats fed a high-fat and high-sucrose diet (HFS) by using multivariate and multiple regression analyses in two types of glucose tolerance tests (GTTs).
31941510	0	90	from	tests	95:99	arg1	rats					104:107	rats	104:107	rats	104:107	Correlation between 12α-hydroxylated bile acids and insulin secretion during glucose tolerance tests in rats fed a high-fat and high-sucrose diet.
31941510	8	91	theme	BAs	1228:1230	arg1	increase					1210:1217	an increase	1207:1217	an increase of 12αOH BAs in enterohepatic circulation, intestinal contents and feces in the HFS-fed rats	1207:1310	We found that an increase of 12αOH BAs in enterohepatic circulation, intestinal contents and feces in the HFS-fed rats compared to those in control rats regardless of no significant increase of total BA concentration in the feces in the test period.
31941510	11	92	theme	organic	1755:1761	arg1	acids					1763:1767	organic acids	1755:1767	organic acids	1755:1767	In contrast, a decrease in the concentration of organic acids was observed in the cecal contents of the HFS-fed rats.
31941510	13	93	theme	12αOH	2065:2069	arg1	BAs					2071:2073	portal 12αOH BAs	2058:2073	portal 12αOH BAs	2058:2073	Moreover, there was a positive correlation between concentration of portal 12αOH BAs and change in insulin concentration of peripheral blood in the IPGTT.
31941510	8	94	theme	enterohepatic	1235:1247	arg1	circulation					1249:1259	enterohepatic circulation	1235:1259	enterohepatic circulation	1235:1259	We found that an increase of 12αOH BAs in enterohepatic circulation, intestinal contents and feces in the HFS-fed rats compared to those in control rats regardless of no significant increase of total BA concentration in the feces in the test period.
31941510	14	95	theme	BA	2265:2266	arg1	metabolism					2268:2277	12αOH BA metabolism	2259:2277	12αOH BA metabolism	2259:2277	CONCLUSION The distribution analysis of BA compositions accompanied by GTTs revealed a close relationship between 12αOH BA metabolism and insulin secretion in GTTs in rats.
31941510	9	96	theme	Fecal	1443:1447	arg1	concentration					1458:1470	Fecal 12αOH BA concentration	1443:1470	Fecal 12αOH BA concentration	1443:1470	Fecal 12αOH BA concentration was positively correlated with maximal insulin level in OGTT and area under curve of insulin in IPGTT.
31941510	6	97	theme	ultra	957:961	arg1	chromatography					987:1000	ultra high-performance liquid chromatography	957:1000	ultra high-performance liquid chromatography	957:1000	Organic acid concentrations in cecal contents were analyzed by using ultra high-performance liquid chromatography with post-column pH buffered electric conductivity method.
31941510	2	98	theme	multiple	510:517	arg1	analyses					530:537	multivariate and multiple regression analyses	493:537	analyses	530:537	The present study was designed to reveal relationships among BA metabolism, glucose tolerance, and cecal organic acids in rats fed a high-fat and high-sucrose diet (HFS) by using multivariate and multiple regression analyses in two types of glucose tolerance tests (GTTs).
31941510	8	99	theme	test	1430:1433	arg1	period					1435:1440	the test period	1426:1440	the test period	1426:1440	We found that an increase of 12αOH BAs in enterohepatic circulation, intestinal contents and feces in the HFS-fed rats compared to those in control rats regardless of no significant increase of total BA concentration in the feces in the test period.
31941510	0	100	theme	glucose	77:83	arg1	tests					95:99	glucose tolerance tests	77:99	glucose tolerance tests in rats	77:107	Correlation between 12α-hydroxylated bile acids and insulin secretion during glucose tolerance tests in rats fed a high-fat and high-sucrose diet.
31941510	13	101	theme	positive	2012:2019	arg1	correlation					2021:2031	a positive correlation	2010:2031	a positive correlation between concentration of portal 12αOH BAs and change in insulin concentration of peripheral blood in the IPGTT	2010:2142	Moreover, there was a positive correlation between concentration of portal 12αOH BAs and change in insulin concentration of peripheral blood in the IPGTT.
31941510	9	102	theme	BA	1455:1456	arg1	concentration					1458:1470	Fecal 12αOH BA concentration	1443:1470	Fecal 12αOH BA concentration	1443:1470	Fecal 12αOH BA concentration was positively correlated with maximal insulin level in OGTT and area under curve of insulin in IPGTT.
31941510	14	103	theme	close	2232:2236	arg1	relationship					2238:2249	a close relationship	2230:2249	a close relationship between 12αOH BA metabolism and insulin secretion in GTTs in rats	2230:2315	CONCLUSION The distribution analysis of BA compositions accompanied by GTTs revealed a close relationship between 12αOH BA metabolism and insulin secretion in GTTs in rats.
31941510	7	104	theme	tissue	1178:1183	arg1	weight					1185:1190	visceral adipose tissue weight	1161:1190	visceral adipose tissue weight	1161:1190	RESULTS A positive correlation of aortic 12αOH BA concentration was observed with energy intake and visceral adipose tissue weight.
31941510	12	105	theme	linear	1834:1839	arg1	analysis					1852:1859	Multiple linear regression analysis	1825:1859	Multiple linear regression analysis in the IPGTT	1825:1872	Multiple linear regression analysis in the IPGTT revealed that the concentrations of aortic 12αOH BA and cecal acetic acid were the predictors of insulin secretion.
31941510	8	106	theme	concentration	1396:1408	arg1	increase					1375:1382	no significant increase	1360:1382	no significant increase of total BA concentration in the feces in the test period	1360:1440	We found that an increase of 12αOH BAs in enterohepatic circulation, intestinal contents and feces in the HFS-fed rats compared to those in control rats regardless of no significant increase of total BA concentration in the feces in the test period.
31941510	7	107	theme	visceral	1161:1168	arg1	weight					1185:1190	visceral adipose tissue weight	1161:1190	visceral adipose tissue weight	1161:1190	RESULTS A positive correlation of aortic 12αOH BA concentration was observed with energy intake and visceral adipose tissue weight.
31941510	6	108	theme	cecal	919:923	arg1	contents					925:932	cecal contents	919:932	cecal contents	919:932	Organic acid concentrations in cecal contents were analyzed by using ultra high-performance liquid chromatography with post-column pH buffered electric conductivity method.
31941510	9	109	theme	maximal	1503:1509	arg1	level					1519:1523	maximal insulin level	1503:1523	maximal insulin level in OGTT	1503:1531	Fecal 12αOH BA concentration was positively correlated with maximal insulin level in OGTT and area under curve of insulin in IPGTT.
31941510	6	110	theme	electric	1031:1038	arg1	method					1053:1058	post-column pH buffered electric conductivity method	1007:1058	post-column pH buffered electric conductivity method	1007:1058	Organic acid concentrations in cecal contents were analyzed by using ultra high-performance liquid chromatography with post-column pH buffered electric conductivity method.
31941510	11	111	located	observed	1773:1780	arg1	contrast					1710:1717	contrast	1710:1717	contrast	1710:1717	In contrast, a decrease in the concentration of organic acids was observed in the cecal contents of the HFS-fed rats.
31941510	11	111	located	observed	1773:1780	arg1	contents					1795:1802	the cecal contents	1785:1802	the cecal contents of the HFS-fed rats	1785:1822	In contrast, a decrease in the concentration of organic acids was observed in the cecal contents of the HFS-fed rats.
31941510	11	111	located	observed	1773:1780	arg2	decrease					1722:1729	a decrease	1720:1729	a decrease in the concentration of organic acids	1720:1767	In contrast, a decrease in the concentration of organic acids was observed in the cecal contents of the HFS-fed rats.
31941510	14	112	theme	compositions	2188:2199	arg1	analysis					2173:2180	The distribution analysis	2156:2180	The distribution analysis of BA compositions accompanied by GTTs	2156:2219	CONCLUSION The distribution analysis of BA compositions accompanied by GTTs revealed a close relationship between 12αOH BA metabolism and insulin secretion in GTTs in rats.
31941510	5	113	theme	high-performance	830:845	arg1	spectrometry					874:885	ultra high-performance liquid chromatography-mass spectrometry	824:885	ultra high-performance liquid chromatography-mass spectrometry	824:885	BAs were analyzed by using ultra high-performance liquid chromatography-mass spectrometry.
31941510	6	114	theme	pH	1019:1020	arg1	method					1053:1058	post-column pH buffered electric conductivity method	1007:1058	post-column pH buffered electric conductivity method	1007:1058	Organic acid concentrations in cecal contents were analyzed by using ultra high-performance liquid chromatography with post-column pH buffered electric conductivity method.
31941510	2	115	theme	cecal	413:417	arg1	acids					427:431	cecal organic acids	413:431	cecal organic acids	413:431	The present study was designed to reveal relationships among BA metabolism, glucose tolerance, and cecal organic acids in rats fed a high-fat and high-sucrose diet (HFS) by using multivariate and multiple regression analyses in two types of glucose tolerance tests (GTTs).
31941510	1	116	dep	intake	236:241	arg1	BAs					309:311	especially 12α-hydroxylated (12αOH) BAs	273:311	especially 12α-hydroxylated (12αOH) BAs	273:311	BACKGROUND Previously, we found a significant relationship in a rat study between energy intake and bile acid (BA) metabolism especially 12α-hydroxylated (12αOH) BAs.
31941510	0	117	theme	bile	37:40	arg1	acids					42:46	12α-hydroxylated bile acids	20:46	12α-hydroxylated bile acids	20:46	Correlation between 12α-hydroxylated bile acids and insulin secretion during glucose tolerance tests in rats fed a high-fat and high-sucrose diet.
31941510	4	118	theme	tolerance	727:735	arg1	IPGTT					743:747	IPGTT	743:747	IPGTT	743:747	Oral glucose tolerance test (OGTT) and intraperitoneal glucose tolerance test (IPGTT) were performed at week 9 and 11, respectively.
31941510	4	118	theme	tolerance	727:735	arg1	test					737:740	intraperitoneal glucose tolerance test	703:740	intraperitoneal glucose tolerance test (IPGTT)	703:748	Oral glucose tolerance test (OGTT) and intraperitoneal glucose tolerance test (IPGTT) were performed at week 9 and 11, respectively.
31941510	5	119	theme	chromatography-mass	854:872	arg1	spectrometry					874:885	ultra high-performance liquid chromatography-mass spectrometry	824:885	ultra high-performance liquid chromatography-mass spectrometry	824:885	BAs were analyzed by using ultra high-performance liquid chromatography-mass spectrometry.
31941510	14	120	theme	12αOH	2259:2263	arg1	metabolism					2268:2277	12αOH BA metabolism	2259:2277	12αOH BA metabolism	2259:2277	CONCLUSION The distribution analysis of BA compositions accompanied by GTTs revealed a close relationship between 12αOH BA metabolism and insulin secretion in GTTs in rats.
31941510	4	121	theme	glucose	669:675	arg1	OGTT					693:696	OGTT	693:696	OGTT	693:696	Oral glucose tolerance test (OGTT) and intraperitoneal glucose tolerance test (IPGTT) were performed at week 9 and 11, respectively.
31941510	4	121	theme	glucose	669:675	arg1	test					687:690	Oral glucose tolerance test	664:690	Oral glucose tolerance test (OGTT)	664:697	Oral glucose tolerance test (OGTT) and intraperitoneal glucose tolerance test (IPGTT) were performed at week 9 and 11, respectively.
31941510	14	122	theme	distribution	2160:2171	arg1	analysis					2173:2180	The distribution analysis	2156:2180	The distribution analysis of BA compositions accompanied by GTTs	2156:2219	CONCLUSION The distribution analysis of BA compositions accompanied by GTTs revealed a close relationship between 12αOH BA metabolism and insulin secretion in GTTs in rats.
31941510	2	123	theme	glucose	390:396	arg1	tolerance					398:406	glucose tolerance	390:406	glucose tolerance	390:406	The present study was designed to reveal relationships among BA metabolism, glucose tolerance, and cecal organic acids in rats fed a high-fat and high-sucrose diet (HFS) by using multivariate and multiple regression analyses in two types of glucose tolerance tests (GTTs).
31941510	7	124	theme	BA	1108:1109	arg1	concentration					1111:1123	aortic 12αOH BA concentration	1095:1123	aortic 12αOH BA concentration	1095:1123	RESULTS A positive correlation of aortic 12αOH BA concentration was observed with energy intake and visceral adipose tissue weight.
31941510	6	125	theme	liquid	980:985	arg1	chromatography					987:1000	ultra high-performance liquid chromatography	957:1000	ultra high-performance liquid chromatography	957:1000	Organic acid concentrations in cecal contents were analyzed by using ultra high-performance liquid chromatography with post-column pH buffered electric conductivity method.
31941510	10	126	theme	positive	1587:1594	arg1	correlation					1596:1606	a positive correlation	1585:1606	a positive correlation between aortic 12αOH BAs concentration and changes in plasma glucose level in both OGTT and IPGTT	1585:1704	There was a positive correlation between aortic 12αOH BAs concentration and changes in plasma glucose level in both OGTT and IPGTT.
31941510	1	127	theme	energy	229:234	arg1	intake					236:241	energy intake	229:241	energy intake	229:241	BACKGROUND Previously, we found a significant relationship in a rat study between energy intake and bile acid (BA) metabolism especially 12α-hydroxylated (12αOH) BAs.
31941510	7	128	theme	aortic	1095:1100	arg1	concentration					1111:1123	aortic 12αOH BA concentration	1095:1123	aortic 12αOH BA concentration	1095:1123	RESULTS A positive correlation of aortic 12αOH BA concentration was observed with energy intake and visceral adipose tissue weight.
31941510	13	129	theme	peripheral	2114:2123	arg1	blood					2125:2129	peripheral blood	2114:2129	peripheral blood	2114:2129	Moreover, there was a positive correlation between concentration of portal 12αOH BAs and change in insulin concentration of peripheral blood in the IPGTT.
31941510	13	130	from	concentration	2097:2109	arg1	IPGTT					2138:2142	the IPGTT	2134:2142	the IPGTT	2134:2142	Moreover, there was a positive correlation between concentration of portal 12αOH BAs and change in insulin concentration of peripheral blood in the IPGTT.
31941510	9	131	theme	insulin	1557:1563	arg1	curve					1548:1552	curve	1548:1552	curve of insulin in IPGTT	1548:1572	Fecal 12αOH BA concentration was positively correlated with maximal insulin level in OGTT and area under curve of insulin in IPGTT.
31941510	3	132	theme	WKAH/HkmSlc	600:610	arg1	rats					612:615	Male WKAH/HkmSlc rats	595:615	Male WKAH/HkmSlc rats	595:615	METHODS Male WKAH/HkmSlc rats were fed with a control or a HFS for 13 weeks.
31941510	8	133	theme	significant	1363:1373	arg1	increase					1375:1382	no significant increase	1360:1382	no significant increase of total BA concentration in the feces in the test period	1360:1440	We found that an increase of 12αOH BAs in enterohepatic circulation, intestinal contents and feces in the HFS-fed rats compared to those in control rats regardless of no significant increase of total BA concentration in the feces in the test period.
31941510	10	134	theme	BAs	1629:1631	arg1	concentration					1633:1645	aortic 12αOH BAs concentration	1616:1645	aortic 12αOH BAs concentration	1616:1645	There was a positive correlation between aortic 12αOH BAs concentration and changes in plasma glucose level in both OGTT and IPGTT.
31941510	12	135	theme	acetic	1936:1941	arg1	acid					1943:1946	cecal acetic acid	1930:1946	cecal acetic acid	1930:1946	Multiple linear regression analysis in the IPGTT revealed that the concentrations of aortic 12αOH BA and cecal acetic acid were the predictors of insulin secretion.
31941510	2	136	from	metabolism	378:387	arg1	rats					436:439	rats	436:439	rats fed a high-fat and high-sucrose diet (HFS) by using multivariate and multiple regression analyses in two types of glucose tolerance tests (GTTs)	436:584	The present study was designed to reveal relationships among BA metabolism, glucose tolerance, and cecal organic acids in rats fed a high-fat and high-sucrose diet (HFS) by using multivariate and multiple regression analyses in two types of glucose tolerance tests (GTTs).
31941510	12	137	theme	BA	1923:1924	arg1	concentrations					1892:1905	the concentrations	1888:1905	the concentrations of aortic 12αOH BA and cecal acetic acid	1888:1946	Multiple linear regression analysis in the IPGTT revealed that the concentrations of aortic 12αOH BA and cecal acetic acid were the predictors of insulin secretion.
31941510	12	137	theme	BA	1923:1924	arg1	predictors					1957:1966	the predictors	1953:1966	the predictors of insulin secretion	1953:1987	Multiple linear regression analysis in the IPGTT revealed that the concentrations of aortic 12αOH BA and cecal acetic acid were the predictors of insulin secretion.
31941510	4	138	theme	glucose	719:725	arg1	IPGTT					743:747	IPGTT	743:747	IPGTT	743:747	Oral glucose tolerance test (OGTT) and intraperitoneal glucose tolerance test (IPGTT) were performed at week 9 and 11, respectively.
31941510	4	138	theme	glucose	719:725	arg1	test					737:740	intraperitoneal glucose tolerance test	703:740	intraperitoneal glucose tolerance test (IPGTT)	703:748	Oral glucose tolerance test (OGTT) and intraperitoneal glucose tolerance test (IPGTT) were performed at week 9 and 11, respectively.
31941510	10	139	theme	glucose	1669:1675	arg1	level					1677:1681	plasma glucose level	1662:1681	plasma glucose level	1662:1681	There was a positive correlation between aortic 12αOH BAs concentration and changes in plasma glucose level in both OGTT and IPGTT.
33809409	3	0	theme	flower	628:633	arg1	abscission					635:644	flower abscission	628:644	flower abscission	628:644	Therefore, we used EPIP peptide to monitor specific modifications accompanied by early steps of flower abscission directly in the AZ.
33809409	1	1	theme	great	127:131	arg1	lupine					115:120	Yellow lupine	108:120	Yellow lupine	108:120	Yellow lupine is a great model for abscission-related research given that excessive flower abortion reduces its yield.
33809409	1	1	theme	great	127:131	arg1	model					133:137	a great model	125:137	a great model for abscission-related research given that excessive flower abortion reduces its yield	125:224	Yellow lupine is a great model for abscission-related research given that excessive flower abortion reduces its yield.
33809409	8	2	theme	metabolic	1404:1412	arg1	switching					1383:1391	the switching	1379:1391	the switching of several metabolic and signaling pathways	1379:1435	Collectively, all these changes indicate the switching of several metabolic and signaling pathways directly in the AZ in response to EPIP, which inevitably leads to flower abscission.
33809409	7	3	theme	EPIP-treated	1321:1332	arg1	AZ					1334:1335	EPIP-treated AZ	1321:1335	EPIP-treated AZ	1321:1335	Notably, the formation of a signaling molecule-phosphatidic acid is induced locally in EPIP-treated AZ.
33809409	0	4	from	Modifications	12:24	arg1	Zone					84:87	the Abscission Zone	69:87	the Abscission Zone of Lupine Flowers	69:105	EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers.
33809409	5	5	theme	redox	849:853	arg1	homeostasis					855:865	redox homeostasis	849:865	redox homeostasis	849:865	The EPIP treatment disrupts redox homeostasis, involving the accumulation of H2O2 and upregulation of the enzymatic antioxidant system including superoxide dismutase, catalase, and ascorbate peroxidase.
33809409	2	6	theme	DEFICIENT	314:322	arg1	INFLORESCENCE					300:312	INFLORESCENCE	300:312	INFLORESCENCE DEFICIENT IN ABSCISSION	300:336	It has been previously shown that the EPIP peptide, a fragment of LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence, is a sufficient molecule to induce flower abortion, however, the question remains: What are the exact changes evoked by this peptide locally in abscission zone (AZ) cells?
33809409	2	6	theme	DEFICIENT	314:322	arg1	sequence					350:357	LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence	293:357	LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence	293:357	It has been previously shown that the EPIP peptide, a fragment of LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence, is a sufficient molecule to induce flower abortion, however, the question remains: What are the exact changes evoked by this peptide locally in abscission zone (AZ) cells?
33809409	6	7	theme	structure	1053:1061	arg1	weakening					1026:1034	A weakening	1024:1034	A weakening of the cell wall structure in response to EPIP	1024:1081	A weakening of the cell wall structure in response to EPIP is reflected by pectin demethylation, while a changing pattern of fatty acids and acyl lipids composition suggests a modification of lipid metabolism.
33809409	6	8	theme	acids	1155:1159	arg1	composition					1177:1187	fatty acids and acyl lipids composition	1149:1187	fatty acids and acyl lipids composition	1149:1187	A weakening of the cell wall structure in response to EPIP is reflected by pectin demethylation, while a changing pattern of fatty acids and acyl lipids composition suggests a modification of lipid metabolism.
33809409	6	9	from	weakening	1026:1034	arg1	response					1066:1073	response	1066:1073	response to EPIP	1066:1081	A weakening of the cell wall structure in response to EPIP is reflected by pectin demethylation, while a changing pattern of fatty acids and acyl lipids composition suggests a modification of lipid metabolism.
33809409	2	10	theme	EPIP	265:268	arg1	molecule					376:383	a sufficient molecule	363:383	a sufficient molecule to induce flower abortion	363:409	It has been previously shown that the EPIP peptide, a fragment of LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence, is a sufficient molecule to induce flower abortion, however, the question remains: What are the exact changes evoked by this peptide locally in abscission zone (AZ) cells?
33809409	2	10	theme	EPIP	265:268	arg1	peptide					270:276	the EPIP peptide	261:276	the EPIP peptide	261:276	It has been previously shown that the EPIP peptide, a fragment of LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence, is a sufficient molecule to induce flower abortion, however, the question remains: What are the exact changes evoked by this peptide locally in abscission zone (AZ) cells?
33809409	2	10	theme	EPIP	265:268	arg1	fragment					281:288	a fragment	279:288	a fragment of LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence	279:357	It has been previously shown that the EPIP peptide, a fragment of LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence, is a sufficient molecule to induce flower abortion, however, the question remains: What are the exact changes evoked by this peptide locally in abscission zone (AZ) cells?
33809409	0	11	theme	Abscission	73:82	arg1	Zone					84:87	the Abscission Zone	69:87	the Abscission Zone of Lupine Flowers	69:105	EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers.
33809409	3	12	theme	early	613:617	arg1	steps					619:623	early steps	613:623	early steps of flower abscission	613:644	Therefore, we used EPIP peptide to monitor specific modifications accompanied by early steps of flower abscission directly in the AZ.
33809409	2	13	theme	AZ	521:522	arg1	cells					525:529	abscission zone (AZ) cells	504:529	abscission zone (AZ) cells	504:529	It has been previously shown that the EPIP peptide, a fragment of LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence, is a sufficient molecule to induce flower abortion, however, the question remains: What are the exact changes evoked by this peptide locally in abscission zone (AZ) cells?
33809409	6	14	theme	metabolism	1222:1231	arg1	modification					1200:1211	a modification	1198:1211	a modification of lipid metabolism	1198:1231	A weakening of the cell wall structure in response to EPIP is reflected by pectin demethylation, while a changing pattern of fatty acids and acyl lipids composition suggests a modification of lipid metabolism.
33809409	3	15	used	used	546:549	arg2	we					543:544	we	543:544	we	543:544	Therefore, we used EPIP peptide to monitor specific modifications accompanied by early steps of flower abscission directly in the AZ.
33809409	0	16	theme	Lupine	92:97	arg1	Flowers					99:105	Lupine Flowers	92:105	Lupine Flowers	92:105	EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers.
33809409	6	17	theme	lipid	1216:1220	arg1	metabolism					1222:1231	lipid metabolism	1216:1231	lipid metabolism	1216:1231	A weakening of the cell wall structure in response to EPIP is reflected by pectin demethylation, while a changing pattern of fatty acids and acyl lipids composition suggests a modification of lipid metabolism.
33809409	5	18	theme	enzymatic	927:935	arg1	system					949:954	the enzymatic antioxidant system	923:954	the enzymatic antioxidant system including superoxide dismutase, catalase, and ascorbate peroxidase	923:1021	The EPIP treatment disrupts redox homeostasis, involving the accumulation of H2O2 and upregulation of the enzymatic antioxidant system including superoxide dismutase, catalase, and ascorbate peroxidase.
33809409	4	19	dep	pathway-HAESA	713:725	arg1	the					709:711	the	709:711	the	709:711	EPIP stimulates the downstream elements of the pathway-HAESA and MITOGEN-ACTIVATED PROTEIN KINASE6 and induces cellular symptoms indicating AZ activation.
33809409	6	20	theme	cell	1043:1046	arg1	structure					1053:1061	the cell wall structure	1039:1061	the cell wall structure	1039:1061	A weakening of the cell wall structure in response to EPIP is reflected by pectin demethylation, while a changing pattern of fatty acids and acyl lipids composition suggests a modification of lipid metabolism.
33809409	3	21	theme	abscission	635:644	arg1	steps					619:623	early steps	613:623	early steps of flower abscission	613:644	Therefore, we used EPIP peptide to monitor specific modifications accompanied by early steps of flower abscission directly in the AZ.
33809409	4	22	theme	cellular	777:784	arg1	symptoms					786:793	cellular symptoms	777:793	cellular symptoms indicating AZ activation	777:818	EPIP stimulates the downstream elements of the pathway-HAESA and MITOGEN-ACTIVATED PROTEIN KINASE6 and induces cellular symptoms indicating AZ activation.
33809409	5	23	theme	antioxidant	937:947	arg1	system					949:954	the enzymatic antioxidant system	923:954	the enzymatic antioxidant system including superoxide dismutase, catalase, and ascorbate peroxidase	923:1021	The EPIP treatment disrupts redox homeostasis, involving the accumulation of H2O2 and upregulation of the enzymatic antioxidant system including superoxide dismutase, catalase, and ascorbate peroxidase.
33809409	1	24	theme	abscission-related	143:160	arg1	research					162:169	abscission-related research	143:169	abscission-related research	143:169	Yellow lupine is a great model for abscission-related research given that excessive flower abortion reduces its yield.
33809409	4	25	theme	AZ	806:807	arg1	activation					809:818	AZ activation	806:818	AZ activation	806:818	EPIP stimulates the downstream elements of the pathway-HAESA and MITOGEN-ACTIVATED PROTEIN KINASE6 and induces cellular symptoms indicating AZ activation.
33809409	2	26	from	DEFICIENT	314:322	arg1	ABSCISSION					327:336	ABSCISSION	327:336	ABSCISSION	327:336	It has been previously shown that the EPIP peptide, a fragment of LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence, is a sufficient molecule to induce flower abortion, however, the question remains: What are the exact changes evoked by this peptide locally in abscission zone (AZ) cells?
33809409	6	27	theme	composition	1177:1187	arg1	pattern					1138:1144	a changing pattern	1127:1144	a changing pattern of fatty acids and acyl lipids composition	1127:1187	A weakening of the cell wall structure in response to EPIP is reflected by pectin demethylation, while a changing pattern of fatty acids and acyl lipids composition suggests a modification of lipid metabolism.
33809409	2	28	theme	sequence	350:357	arg1	peptide					270:276	the EPIP peptide	261:276	the EPIP peptide	261:276	It has been previously shown that the EPIP peptide, a fragment of LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence, is a sufficient molecule to induce flower abortion, however, the question remains: What are the exact changes evoked by this peptide locally in abscission zone (AZ) cells?
33809409	2	28	theme	sequence	350:357	arg1	fragment					281:288	a fragment	279:288	a fragment of LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence	279:357	It has been previously shown that the EPIP peptide, a fragment of LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence, is a sufficient molecule to induce flower abortion, however, the question remains: What are the exact changes evoked by this peptide locally in abscission zone (AZ) cells?
33809409	0	29	theme	EPIP-Evoked	0:10	arg1	Modifications					12:24	EPIP-Evoked Modifications	0:24	EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers	0:105	EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers.
33809409	6	30	theme	fatty	1149:1153	arg1	acids					1155:1159	fatty acids	1149:1159	fatty acids	1149:1159	A weakening of the cell wall structure in response to EPIP is reflected by pectin demethylation, while a changing pattern of fatty acids and acyl lipids composition suggests a modification of lipid metabolism.
33809409	5	31	theme	H2O2	898:901	arg1	upregulation					907:918	upregulation	907:918	upregulation of the enzymatic antioxidant system including superoxide dismutase, catalase, and ascorbate peroxidase	907:1021	The EPIP treatment disrupts redox homeostasis, involving the accumulation of H2O2 and upregulation of the enzymatic antioxidant system including superoxide dismutase, catalase, and ascorbate peroxidase.
33809409	5	31	theme	H2O2	898:901	arg1	accumulation					882:893	the accumulation	878:893	the accumulation of H2O2	878:901	The EPIP treatment disrupts redox homeostasis, involving the accumulation of H2O2 and upregulation of the enzymatic antioxidant system including superoxide dismutase, catalase, and ascorbate peroxidase.
33809409	2	32	theme	amino-acid	339:348	arg1	INFLORESCENCE					300:312	INFLORESCENCE	300:312	INFLORESCENCE DEFICIENT IN ABSCISSION	300:336	It has been previously shown that the EPIP peptide, a fragment of LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence, is a sufficient molecule to induce flower abortion, however, the question remains: What are the exact changes evoked by this peptide locally in abscission zone (AZ) cells?
33809409	2	32	theme	amino-acid	339:348	arg1	sequence					350:357	LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence	293:357	LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence	293:357	It has been previously shown that the EPIP peptide, a fragment of LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence, is a sufficient molecule to induce flower abortion, however, the question remains: What are the exact changes evoked by this peptide locally in abscission zone (AZ) cells?
33809409	0	33	theme	Redox	29:33	arg1	Lipid					36:40	Redox, Lipid, and Pectin Homeostasis	29:64	Lipid	36:40	EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers.
33809409	5	34	theme	superoxide	966:975	arg1	dismutase					977:985	superoxide dismutase	966:985	superoxide dismutase	966:985	The EPIP treatment disrupts redox homeostasis, involving the accumulation of H2O2 and upregulation of the enzymatic antioxidant system including superoxide dismutase, catalase, and ascorbate peroxidase.
33809409	2	35	from	ABSCISSION	327:336	arg1	DEFICIENT					314:322	DEFICIENT	314:322	DEFICIENT	314:322	It has been previously shown that the EPIP peptide, a fragment of LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence, is a sufficient molecule to induce flower abortion, however, the question remains: What are the exact changes evoked by this peptide locally in abscission zone (AZ) cells?
33809409	6	36	theme	pectin	1099:1104	arg1	demethylation					1106:1118	pectin demethylation	1099:1118	pectin demethylation	1099:1118	A weakening of the cell wall structure in response to EPIP is reflected by pectin demethylation, while a changing pattern of fatty acids and acyl lipids composition suggests a modification of lipid metabolism.
33809409	0	37	theme	Flowers	99:105	arg1	Zone					84:87	the Abscission Zone	69:87	the Abscission Zone of Lupine Flowers	69:105	EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers.
33809409	6	38	theme	wall	1048:1051	arg1	structure					1053:1061	the cell wall structure	1039:1061	the cell wall structure	1039:1061	A weakening of the cell wall structure in response to EPIP is reflected by pectin demethylation, while a changing pattern of fatty acids and acyl lipids composition suggests a modification of lipid metabolism.
33809409	1	39	theme	excessive	182:190	arg1	abortion					199:206	excessive flower abortion	182:206	excessive flower abortion	182:206	Yellow lupine is a great model for abscission-related research given that excessive flower abortion reduces its yield.
33809409	0	40	theme	Lipid	36:40	arg1	Modifications					12:24	EPIP-Evoked Modifications	0:24	EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers	0:105	EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers.
33809409	1	41	theme	flower	192:197	arg1	abortion					199:206	excessive flower abortion	182:206	excessive flower abortion	182:206	Yellow lupine is a great model for abscission-related research given that excessive flower abortion reduces its yield.
33809409	4	42	theme	KINASE6	757:763	arg1	elements					697:704	the downstream elements	682:704	the downstream elements of the pathway-HAESA and MITOGEN-ACTIVATED PROTEIN KINASE6	682:763	EPIP stimulates the downstream elements of the pathway-HAESA and MITOGEN-ACTIVATED PROTEIN KINASE6 and induces cellular symptoms indicating AZ activation.
33809409	5	43	theme	EPIP	825:828	arg1	treatment					830:838	The EPIP treatment	821:838	The EPIP treatment	821:838	The EPIP treatment disrupts redox homeostasis, involving the accumulation of H2O2 and upregulation of the enzymatic antioxidant system including superoxide dismutase, catalase, and ascorbate peroxidase.
33809409	2	44	theme	sufficient	365:374	arg1	molecule					376:383	a sufficient molecule	363:383	a sufficient molecule to induce flower abortion	363:409	It has been previously shown that the EPIP peptide, a fragment of LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence, is a sufficient molecule to induce flower abortion, however, the question remains: What are the exact changes evoked by this peptide locally in abscission zone (AZ) cells?
33809409	2	44	theme	sufficient	365:374	arg1	peptide					270:276	the EPIP peptide	261:276	the EPIP peptide	261:276	It has been previously shown that the EPIP peptide, a fragment of LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence, is a sufficient molecule to induce flower abortion, however, the question remains: What are the exact changes evoked by this peptide locally in abscission zone (AZ) cells?
33809409	7	45	theme	acid	1294:1297	arg1	formation					1247:1255	the formation	1243:1255	the formation of a signaling molecule-phosphatidic acid	1243:1297	Notably, the formation of a signaling molecule-phosphatidic acid is induced locally in EPIP-treated AZ.
33809409	3	46	theme	EPIP	551:554	arg1	peptide					556:562	EPIP peptide	551:562	EPIP peptide	551:562	Therefore, we used EPIP peptide to monitor specific modifications accompanied by early steps of flower abscission directly in the AZ.
33809409	2	47	theme	flower	395:400	arg1	abortion					402:409	flower abortion	395:409	flower abortion	395:409	It has been previously shown that the EPIP peptide, a fragment of LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence, is a sufficient molecule to induce flower abortion, however, the question remains: What are the exact changes evoked by this peptide locally in abscission zone (AZ) cells?
33809409	2	48	theme	exact	456:460	arg1	What					443:446	What	443:446	What	443:446	It has been previously shown that the EPIP peptide, a fragment of LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence, is a sufficient molecule to induce flower abortion, however, the question remains: What are the exact changes evoked by this peptide locally in abscission zone (AZ) cells?
33809409	2	48	theme	exact	456:460	arg1	changes					462:468	the exact changes	452:468	the exact changes evoked by this peptide locally in abscission zone (AZ) cells	452:529	It has been previously shown that the EPIP peptide, a fragment of LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence, is a sufficient molecule to induce flower abortion, however, the question remains: What are the exact changes evoked by this peptide locally in abscission zone (AZ) cells?
33809409	8	49	theme	pathways	1428:1435	arg1	switching					1383:1391	the switching	1379:1391	the switching of several metabolic and signaling pathways	1379:1435	Collectively, all these changes indicate the switching of several metabolic and signaling pathways directly in the AZ in response to EPIP, which inevitably leads to flower abscission.
33809409	8	50	theme	signaling	1418:1426	arg1	pathways					1428:1435	signaling pathways	1418:1435	signaling pathways	1418:1435	Collectively, all these changes indicate the switching of several metabolic and signaling pathways directly in the AZ in response to EPIP, which inevitably leads to flower abscission.
33809409	6	51	theme	lipids	1170:1175	arg1	composition					1177:1187	fatty acids and acyl lipids composition	1149:1187	fatty acids and acyl lipids composition	1149:1187	A weakening of the cell wall structure in response to EPIP is reflected by pectin demethylation, while a changing pattern of fatty acids and acyl lipids composition suggests a modification of lipid metabolism.
33809409	6	52	theme	changing	1129:1136	arg1	pattern					1138:1144	a changing pattern	1127:1144	a changing pattern of fatty acids and acyl lipids composition	1127:1187	A weakening of the cell wall structure in response to EPIP is reflected by pectin demethylation, while a changing pattern of fatty acids and acyl lipids composition suggests a modification of lipid metabolism.
33809409	2	53	theme	zone	515:518	arg1	cells					525:529	abscission zone (AZ) cells	504:529	abscission zone (AZ) cells	504:529	It has been previously shown that the EPIP peptide, a fragment of LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence, is a sufficient molecule to induce flower abortion, however, the question remains: What are the exact changes evoked by this peptide locally in abscission zone (AZ) cells?
33809409	5	54	theme	ascorbate	1002:1010	arg1	peroxidase					1012:1021	ascorbate peroxidase	1002:1021	ascorbate peroxidase	1002:1021	The EPIP treatment disrupts redox homeostasis, involving the accumulation of H2O2 and upregulation of the enzymatic antioxidant system including superoxide dismutase, catalase, and ascorbate peroxidase.
33809409	6	55	theme	acyl	1165:1168	arg1	lipids					1170:1175	acyl lipids	1165:1175	acyl lipids	1165:1175	A weakening of the cell wall structure in response to EPIP is reflected by pectin demethylation, while a changing pattern of fatty acids and acyl lipids composition suggests a modification of lipid metabolism.
33809409	6	56	dep	reflected	1086:1094	arg1	while					1121:1125	while	1121:1125	while	1121:1125	A weakening of the cell wall structure in response to EPIP is reflected by pectin demethylation, while a changing pattern of fatty acids and acyl lipids composition suggests a modification of lipid metabolism.
33809409	8	57	dep	EPIP	1471:1474	arg1	response					1459:1466	response	1459:1466	response	1459:1466	Collectively, all these changes indicate the switching of several metabolic and signaling pathways directly in the AZ in response to EPIP, which inevitably leads to flower abscission.
33809409	8	58	theme	flower	1503:1508	arg1	abscission					1510:1519	flower abscission	1503:1519	flower abscission	1503:1519	Collectively, all these changes indicate the switching of several metabolic and signaling pathways directly in the AZ in response to EPIP, which inevitably leads to flower abscission.
33809409	2	59	theme	abscission	504:513	arg1	cells					525:529	abscission zone (AZ) cells	504:529	abscission zone (AZ) cells	504:529	It has been previously shown that the EPIP peptide, a fragment of LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence, is a sufficient molecule to induce flower abortion, however, the question remains: What are the exact changes evoked by this peptide locally in abscission zone (AZ) cells?
33809409	5	60	theme	system	949:954	arg1	upregulation					907:918	upregulation	907:918	upregulation of the enzymatic antioxidant system including superoxide dismutase, catalase, and ascorbate peroxidase	907:1021	The EPIP treatment disrupts redox homeostasis, involving the accumulation of H2O2 and upregulation of the enzymatic antioxidant system including superoxide dismutase, catalase, and ascorbate peroxidase.
33809409	5	60	theme	system	949:954	arg1	accumulation					882:893	the accumulation	878:893	the accumulation of H2O2	878:901	The EPIP treatment disrupts redox homeostasis, involving the accumulation of H2O2 and upregulation of the enzymatic antioxidant system including superoxide dismutase, catalase, and ascorbate peroxidase.
33809409	4	61	theme	pathway-HAESA	713:725	arg1	elements					697:704	the downstream elements	682:704	the downstream elements of the pathway-HAESA and MITOGEN-ACTIVATED PROTEIN KINASE6	682:763	EPIP stimulates the downstream elements of the pathway-HAESA and MITOGEN-ACTIVATED PROTEIN KINASE6 and induces cellular symptoms indicating AZ activation.
33809409	4	62	theme	PROTEIN	749:755	arg1	KINASE6					757:763	MITOGEN-ACTIVATED PROTEIN KINASE6	731:763	MITOGEN-ACTIVATED PROTEIN KINASE6	731:763	EPIP stimulates the downstream elements of the pathway-HAESA and MITOGEN-ACTIVATED PROTEIN KINASE6 and induces cellular symptoms indicating AZ activation.
33809409	1	63	theme	Yellow	108:113	arg1	lupine					115:120	Yellow lupine	108:120	Yellow lupine	108:120	Yellow lupine is a great model for abscission-related research given that excessive flower abortion reduces its yield.
33809409	1	63	theme	Yellow	108:113	arg1	model					133:137	a great model	125:137	a great model for abscission-related research given that excessive flower abortion reduces its yield	125:224	Yellow lupine is a great model for abscission-related research given that excessive flower abortion reduces its yield.
33809409	3	64	theme	specific	575:582	arg1	modifications					584:596	specific modifications	575:596	specific modifications accompanied by early steps of flower abscission	575:644	Therefore, we used EPIP peptide to monitor specific modifications accompanied by early steps of flower abscission directly in the AZ.
33809409	4	65	theme	downstream	686:695	arg1	elements					697:704	the downstream elements	682:704	the downstream elements of the pathway-HAESA and MITOGEN-ACTIVATED PROTEIN KINASE6	682:763	EPIP stimulates the downstream elements of the pathway-HAESA and MITOGEN-ACTIVATED PROTEIN KINASE6 and induces cellular symptoms indicating AZ activation.
33809409	7	66	theme	molecule-phosphatidic	1272:1292	arg1	acid					1294:1297	a signaling molecule-phosphatidic acid	1260:1297	a signaling molecule-phosphatidic acid	1260:1297	Notably, the formation of a signaling molecule-phosphatidic acid is induced locally in EPIP-treated AZ.
33809409	7	67	theme	signaling	1262:1270	arg1	acid					1294:1297	a signaling molecule-phosphatidic acid	1260:1297	a signaling molecule-phosphatidic acid	1260:1297	Notably, the formation of a signaling molecule-phosphatidic acid is induced locally in EPIP-treated AZ.
33809409	2	68	theme	LlIDL	293:297	arg1	INFLORESCENCE					300:312	INFLORESCENCE	300:312	INFLORESCENCE DEFICIENT IN ABSCISSION	300:336	It has been previously shown that the EPIP peptide, a fragment of LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence, is a sufficient molecule to induce flower abortion, however, the question remains: What are the exact changes evoked by this peptide locally in abscission zone (AZ) cells?
33809409	2	68	theme	LlIDL	293:297	arg1	sequence					350:357	LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence	293:357	LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence	293:357	It has been previously shown that the EPIP peptide, a fragment of LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence, is a sufficient molecule to induce flower abortion, however, the question remains: What are the exact changes evoked by this peptide locally in abscission zone (AZ) cells?
33809409	0	69	theme	Homeostasis	54:64	arg1	Modifications					12:24	EPIP-Evoked Modifications	0:24	EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers	0:105	EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers.
33809409	4	70	theme	MITOGEN-ACTIVATED	731:747	arg1	KINASE6					757:763	MITOGEN-ACTIVATED PROTEIN KINASE6	731:763	MITOGEN-ACTIVATED PROTEIN KINASE6	731:763	EPIP stimulates the downstream elements of the pathway-HAESA and MITOGEN-ACTIVATED PROTEIN KINASE6 and induces cellular symptoms indicating AZ activation.
33809409	0	71	theme	Pectin	47:52	arg1	Homeostasis					54:64	Redox, Lipid, and Pectin Homeostasis	29:64	Homeostasis	54:64	EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers.
32375947	8	0	theme	rRNA	965:968	arg1	sequence					975:982	16S rRNA gene sequence	961:982	16S rRNA gene sequence	961:982	Comparisons of 16S rRNA gene sequence indicated that 3 H-10T was most closely related to Bacillus mesophilus SA4T (96.30 %), Bacillus ginsengihumi Gsoil 114T (96.27 %) and Bacillus shackletonii LMG 18435T (96.27 %).
32375947	2	1	theme	Gram-stain-positive	95:113	arg1	bacterium					148:156	A Gram-stain-positive, strictly aerobic and rod-shaped bacterium	93:156	A Gram-stain-positive, strictly aerobic and rod-shaped bacterium	93:156	A Gram-stain-positive, strictly aerobic and rod-shaped bacterium, designated as 3 H-10T, was isolated from a yellow water sample collected from the manufacturing process of strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China).
32375947	8	2	theme	sequence	975:982	arg1	Comparisons					946:956	Comparisons	946:956	Comparisons of 16S rRNA gene sequence	946:982	Comparisons of 16S rRNA gene sequence indicated that 3 H-10T was most closely related to Bacillus mesophilus SA4T (96.30 %), Bacillus ginsengihumi Gsoil 114T (96.27 %) and Bacillus shackletonii LMG 18435T (96.27 %).
32375947	2	3	from	region	304:309	arg1	process					255:261	the manufacturing process	237:261	the manufacturing process of strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China)	237:340	A Gram-stain-positive, strictly aerobic and rod-shaped bacterium, designated as 3 H-10T, was isolated from a yellow water sample collected from the manufacturing process of strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China).
32375947	4	4	theme	optimum	522:528	arg1	pH					509:510	pH 6.0-10.0	509:519	pH 6.0-10.0 (optimum growth at pH 8.0)	509:546	The isolate was able to grow at temperatures of 20-45 °C (optimum growth at 37 °C), at pH 6.0-10.0 (optimum growth at pH 8.0) and in the presence of 0-2 % (w/v) NaCl (optimum growth with 0 % NaCl).
32375947	4	4	theme	optimum	522:528	arg1	pH					540:541	optimum growth at pH 8.0	522:545	optimum growth at pH 8.0	522:545	The isolate was able to grow at temperatures of 20-45 °C (optimum growth at 37 °C), at pH 6.0-10.0 (optimum growth at pH 8.0) and in the presence of 0-2 % (w/v) NaCl (optimum growth with 0 % NaCl).
32375947	10	5	theme	DNA	1330:1332	arg1	content					1338:1344	The genomic DNA G+C content	1318:1344	The genomic DNA G+C content	1318:1344	The genomic DNA G+C content was 35.4 mol%.
32375947	10	5	theme	DNA	1330:1332	arg1	%					1358:1358	35.4 mol%	1350:1358	35.4 mol%	1350:1358	The genomic DNA G+C content was 35.4 mol%.
32375947	11	6	theme	phylogenetic	1412:1423	arg1	properties					1425:1434	its phenotypic, chemotaxonomic and phylogenetic properties	1377:1434	its phenotypic, chemotaxonomic and phylogenetic properties	1377:1434	On the basis of its phenotypic, chemotaxonomic and phylogenetic properties, strain 3 H-10T represents a novel species of the genus Bacillus, for which the name Bacillus aquiflavi sp.
32375947	7	7	theme	fatty	934:938	arg1	 0					908:909	 0	908:909	 0 (60.7 %)	908:918	MK-7 was predominant menaquinone and iso-C15 : 0 (60.7 %) was the major fatty acid.
32375947	7	7	theme	fatty	934:938	arg1	acid					940:943	the major fatty acid	924:943	the major fatty acid	924:943	MK-7 was predominant menaquinone and iso-C15 : 0 (60.7 %) was the major fatty acid.
32375947	2	8	theme	strong	266:271	arg1	baijiu					288:293	strong flavor Chinese baijiu	266:293	strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China)	266:340	A Gram-stain-positive, strictly aerobic and rod-shaped bacterium, designated as 3 H-10T, was isolated from a yellow water sample collected from the manufacturing process of strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China).
32375947	6	9	theme	polar	738:742	arg1	lipids					744:749	The main polar lipids	729:749	The main polar lipids of 3 H-10T	729:760	The main polar lipids of 3 H-10T included diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and phosphatidylethanolamine (PE).
32375947	11	10	theme	chemotaxonomic	1393:1406	arg1	properties					1425:1434	its phenotypic, chemotaxonomic and phylogenetic properties	1377:1434	its phenotypic, chemotaxonomic and phylogenetic properties	1377:1434	On the basis of its phenotypic, chemotaxonomic and phylogenetic properties, strain 3 H-10T represents a novel species of the genus Bacillus, for which the name Bacillus aquiflavi sp.
32375947	0	11	theme	Bacillus	0:7	arg1	sp					19:20	Bacillus aquiflavi sp	0:20	Bacillus aquiflavi sp.	0:21	Bacillus aquiflavi sp.
32375947	5	12	theme	amino	717:721	arg1	acid					682:685	meso-diaminopimelic acid	662:685	meso-diaminopimelic acid (meso-DAP)	662:696	Ribose was the major cell-wall sugar, and meso-diaminopimelic acid (meso-DAP) was the diagnostic amino acid.
32375947	5	12	theme	amino	717:721	arg1	acid					723:726	the diagnostic amino acid	702:726	the diagnostic amino acid	702:726	Ribose was the major cell-wall sugar, and meso-diaminopimelic acid (meso-DAP) was the diagnostic amino acid.
32375947	4	13	theme	%	575:575	arg1	NaCl					583:586	0-2 % (w/v) NaCl	571:586	0-2 % (w/v) NaCl (optimum growth with 0 % NaCl)	571:617	The isolate was able to grow at temperatures of 20-45 °C (optimum growth at 37 °C), at pH 6.0-10.0 (optimum growth at pH 8.0) and in the presence of 0-2 % (w/v) NaCl (optimum growth with 0 % NaCl).
32375947	10	14	theme	35.4 mol	1350:1357	arg1	content					1338:1344	The genomic DNA G+C content	1318:1344	The genomic DNA G+C content	1318:1344	The genomic DNA G+C content was 35.4 mol%.
32375947	10	14	theme	35.4 mol	1350:1357	arg1	%					1358:1358	35.4 mol%	1350:1358	35.4 mol%	1350:1358	The genomic DNA G+C content was 35.4 mol%.
32375947	11	15	theme	Bacillus	1521:1528	arg1	sp					1540:1541	the name Bacillus aquiflavi sp	1512:1541	the name Bacillus aquiflavi sp	1512:1541	On the basis of its phenotypic, chemotaxonomic and phylogenetic properties, strain 3 H-10T represents a novel species of the genus Bacillus, for which the name Bacillus aquiflavi sp.
32375947	2	16	theme	Chinese	280:286	arg1	baijiu					288:293	strong flavor Chinese baijiu	266:293	strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China)	266:340	A Gram-stain-positive, strictly aerobic and rod-shaped bacterium, designated as 3 H-10T, was isolated from a yellow water sample collected from the manufacturing process of strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China).
32375947	8	17	theme	ginsengihumi	1080:1091	arg1	114T					1099:1102	Bacillus ginsengihumi Gsoil 114T	1071:1102	Bacillus ginsengihumi Gsoil 114T (96.27 %)	1071:1112	Comparisons of 16S rRNA gene sequence indicated that 3 H-10T was most closely related to Bacillus mesophilus SA4T (96.30 %), Bacillus ginsengihumi Gsoil 114T (96.27 %) and Bacillus shackletonii LMG 18435T (96.27 %).
32375947	8	17	theme	ginsengihumi	1080:1091	arg1	%					1111:1111	96.27 %	1105:1111	96.27 %	1105:1111	Comparisons of 16S rRNA gene sequence indicated that 3 H-10T was most closely related to Bacillus mesophilus SA4T (96.30 %), Bacillus ginsengihumi Gsoil 114T (96.27 %) and Bacillus shackletonii LMG 18435T (96.27 %).
32375947	2	18	theme	water	209:213	arg1	sample					215:220	a yellow water sample	200:220	a yellow water sample collected from the manufacturing process of strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China)	200:340	A Gram-stain-positive, strictly aerobic and rod-shaped bacterium, designated as 3 H-10T, was isolated from a yellow water sample collected from the manufacturing process of strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China).
32375947	4	19	theme	optimum	589:595	arg1	growth					597:602	optimum growth	589:602	optimum growth with 0 % NaCl	589:616	The isolate was able to grow at temperatures of 20-45 °C (optimum growth at 37 °C), at pH 6.0-10.0 (optimum growth at pH 8.0) and in the presence of 0-2 % (w/v) NaCl (optimum growth with 0 % NaCl).
32375947	3	20	with	cells	392:396	arg1	sporangia					411:419	swollen sporangia	403:419	swollen sporangia	403:419	Oval endospores were formed at the subtermini of cells with swollen sporangia.
32375947	11	21	theme	Bacillus	1492:1499	arg1	species					1471:1477	a novel species	1463:1477	a novel species	1463:1477	On the basis of its phenotypic, chemotaxonomic and phylogenetic properties, strain 3 H-10T represents a novel species of the genus Bacillus, for which the name Bacillus aquiflavi sp.
32375947	9	22	theme	69.56	1278:1282	arg1	%					1301:1301	69.56, 70.19 and 70.67 %	1278:1301	%	1301:1301	The average nucleotide identity (ANI) values between strain 3 H-10T and the three type strains mentioned above were 69.56, 70.19 and 70.67 %, respectively.
32375947	4	23	theme	w/v	578:580	arg1	NaCl					583:586	0-2 % (w/v) NaCl	571:586	0-2 % (w/v) NaCl (optimum growth with 0 % NaCl)	571:617	The isolate was able to grow at temperatures of 20-45 °C (optimum growth at 37 °C), at pH 6.0-10.0 (optimum growth at pH 8.0) and in the presence of 0-2 % (w/v) NaCl (optimum growth with 0 % NaCl).
32375947	2	24	from	baijiu	288:293	arg1	region					304:309	Yibin region	298:309	Yibin region of Sichuan province (PR China)	298:340	A Gram-stain-positive, strictly aerobic and rod-shaped bacterium, designated as 3 H-10T, was isolated from a yellow water sample collected from the manufacturing process of strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China).
32375947	4	25	theme	NaCl	583:586	arg1	presence					559:566	the presence	555:566	the presence of 0-2 % (w/v) NaCl (optimum growth with 0 % NaCl)	555:617	The isolate was able to grow at temperatures of 20-45 °C (optimum growth at 37 °C), at pH 6.0-10.0 (optimum growth at pH 8.0) and in the presence of 0-2 % (w/v) NaCl (optimum growth with 0 % NaCl).
32375947	13	26	theme	24755T=JCM	1615:1624	arg1	33703T					1626:1631	=CICC 24755T=JCM 33703T	1609:1631	=CICC 24755T=JCM 33703T	1609:1631	The type strain is Bacillus aquiflavi 3 H-10T (=CICC 24755T=JCM 33703T).
32375947	13	26	theme	24755T=JCM	1615:1624	arg1	3 H-10T					1600:1606	Bacillus aquiflavi 3 H-10T	1581:1606	Bacillus aquiflavi 3 H-10T (=CICC 24755T=JCM 33703T)	1581:1632	The type strain is Bacillus aquiflavi 3 H-10T (=CICC 24755T=JCM 33703T).
32375947	9	27	theme	70.67 	1295:1300	arg1	%					1301:1301	69.56, 70.19 and 70.67 %	1278:1301	%	1301:1301	The average nucleotide identity (ANI) values between strain 3 H-10T and the three type strains mentioned above were 69.56, 70.19 and 70.67 %, respectively.
32375947	8	28	theme	LMG	1140:1142	arg1	18435T					1144:1149	Bacillus shackletonii LMG 18435T	1118:1149	Bacillus shackletonii LMG 18435T (96.27 %)	1118:1159	Comparisons of 16S rRNA gene sequence indicated that 3 H-10T was most closely related to Bacillus mesophilus SA4T (96.30 %), Bacillus ginsengihumi Gsoil 114T (96.27 %) and Bacillus shackletonii LMG 18435T (96.27 %).
32375947	8	28	theme	LMG	1140:1142	arg1	%					1158:1158	96.27 %	1152:1158	96.27 %	1152:1158	Comparisons of 16S rRNA gene sequence indicated that 3 H-10T was most closely related to Bacillus mesophilus SA4T (96.30 %), Bacillus ginsengihumi Gsoil 114T (96.27 %) and Bacillus shackletonii LMG 18435T (96.27 %).
32375947	13	29	theme	aquiflavi	1590:1598	arg1	strain					1571:1576	The type strain	1562:1576	The type strain	1562:1576	The type strain is Bacillus aquiflavi 3 H-10T (=CICC 24755T=JCM 33703T).
32375947	13	29	theme	aquiflavi	1590:1598	arg1	33703T					1626:1631	=CICC 24755T=JCM 33703T	1609:1631	=CICC 24755T=JCM 33703T	1609:1631	The type strain is Bacillus aquiflavi 3 H-10T (=CICC 24755T=JCM 33703T).
32375947	13	29	theme	aquiflavi	1590:1598	arg1	3 H-10T					1600:1606	Bacillus aquiflavi 3 H-10T	1581:1606	Bacillus aquiflavi 3 H-10T (=CICC 24755T=JCM 33703T)	1581:1632	The type strain is Bacillus aquiflavi 3 H-10T (=CICC 24755T=JCM 33703T).
32375947	4	30	theme	%	611:611	arg1	NaCl					613:616	0 % NaCl	609:616	0 % NaCl	609:616	The isolate was able to grow at temperatures of 20-45 °C (optimum growth at 37 °C), at pH 6.0-10.0 (optimum growth at pH 8.0) and in the presence of 0-2 % (w/v) NaCl (optimum growth with 0 % NaCl).
32375947	5	31	theme	meso-diaminopimelic	662:680	arg1	acid					723:726	the diagnostic amino acid	702:726	the diagnostic amino acid	702:726	Ribose was the major cell-wall sugar, and meso-diaminopimelic acid (meso-DAP) was the diagnostic amino acid.
32375947	5	31	theme	meso-diaminopimelic	662:680	arg1	meso-DAP					688:695	meso-DAP	688:695	meso-DAP	688:695	Ribose was the major cell-wall sugar, and meso-diaminopimelic acid (meso-DAP) was the diagnostic amino acid.
32375947	5	31	theme	meso-diaminopimelic	662:680	arg1	acid					682:685	meso-diaminopimelic acid	662:685	meso-diaminopimelic acid (meso-DAP)	662:696	Ribose was the major cell-wall sugar, and meso-diaminopimelic acid (meso-DAP) was the diagnostic amino acid.
32375947	1	32	theme	Chinese	77:83	arg1	baijiu					85:90	strongly flavored Chinese baijiu	59:90	strongly flavored Chinese baijiu	59:90	nov., isolated from yellow water of strongly flavored Chinese baijiu.
32375947	2	33	dep	province	322:329	arg1	China					335:339	PR China	332:339	Sichuan province (PR China)	314:340	A Gram-stain-positive, strictly aerobic and rod-shaped bacterium, designated as 3 H-10T, was isolated from a yellow water sample collected from the manufacturing process of strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China).
32375947	13	34	theme	type	1566:1569	arg1	strain					1571:1576	The type strain	1562:1576	The type strain	1562:1576	The type strain is Bacillus aquiflavi 3 H-10T (=CICC 24755T=JCM 33703T).
32375947	13	34	theme	type	1566:1569	arg1	3 H-10T					1600:1606	Bacillus aquiflavi 3 H-10T	1581:1606	Bacillus aquiflavi 3 H-10T (=CICC 24755T=JCM 33703T)	1581:1632	The type strain is Bacillus aquiflavi 3 H-10T (=CICC 24755T=JCM 33703T).
32375947	5	35	theme	cell-wall	641:649	arg1	sugar					651:655	the major cell-wall sugar	631:655	the major cell-wall sugar	631:655	Ribose was the major cell-wall sugar, and meso-diaminopimelic acid (meso-DAP) was the diagnostic amino acid.
32375947	5	35	theme	cell-wall	641:649	arg1	Ribose					620:625	Ribose	620:625	Ribose	620:625	Ribose was the major cell-wall sugar, and meso-diaminopimelic acid (meso-DAP) was the diagnostic amino acid.
32375947	4	36	theme	optimum	480:486	arg1	37 °C					498:502	optimum growth at 37 °C	480:502	optimum growth at 37 °C	480:502	The isolate was able to grow at temperatures of 20-45 °C (optimum growth at 37 °C), at pH 6.0-10.0 (optimum growth at pH 8.0) and in the presence of 0-2 % (w/v) NaCl (optimum growth with 0 % NaCl).
32375947	4	36	theme	optimum	480:486	arg1	20-45 °C					470:477	20-45 °C	470:477	20-45 °C (optimum growth at 37 °C)	470:503	The isolate was able to grow at temperatures of 20-45 °C (optimum growth at 37 °C), at pH 6.0-10.0 (optimum growth at pH 8.0) and in the presence of 0-2 % (w/v) NaCl (optimum growth with 0 % NaCl).
32375947	11	37	theme	strain	1437:1442	arg1	3 H-10T					1444:1450	strain 3 H-10T	1437:1450	strain 3 H-10T	1437:1450	On the basis of its phenotypic, chemotaxonomic and phylogenetic properties, strain 3 H-10T represents a novel species of the genus Bacillus, for which the name Bacillus aquiflavi sp.
32375947	2	38	theme	province	322:329	arg1	region					304:309	Yibin region	298:309	Yibin region of Sichuan province (PR China)	298:340	A Gram-stain-positive, strictly aerobic and rod-shaped bacterium, designated as 3 H-10T, was isolated from a yellow water sample collected from the manufacturing process of strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China).
32375947	4	39	theme	growth	488:493	arg1	37 °C					498:502	optimum growth at 37 °C	480:502	optimum growth at 37 °C	480:502	The isolate was able to grow at temperatures of 20-45 °C (optimum growth at 37 °C), at pH 6.0-10.0 (optimum growth at pH 8.0) and in the presence of 0-2 % (w/v) NaCl (optimum growth with 0 % NaCl).
32375947	4	39	theme	growth	488:493	arg1	20-45 °C					470:477	20-45 °C	470:477	20-45 °C (optimum growth at 37 °C)	470:503	The isolate was able to grow at temperatures of 20-45 °C (optimum growth at 37 °C), at pH 6.0-10.0 (optimum growth at pH 8.0) and in the presence of 0-2 % (w/v) NaCl (optimum growth with 0 % NaCl).
32375947	4	40	theme	growth	530:535	arg1	pH					509:510	pH 6.0-10.0	509:519	pH 6.0-10.0 (optimum growth at pH 8.0)	509:546	The isolate was able to grow at temperatures of 20-45 °C (optimum growth at 37 °C), at pH 6.0-10.0 (optimum growth at pH 8.0) and in the presence of 0-2 % (w/v) NaCl (optimum growth with 0 % NaCl).
32375947	4	40	theme	growth	530:535	arg1	pH					540:541	optimum growth at pH 8.0	522:545	optimum growth at pH 8.0	522:545	The isolate was able to grow at temperatures of 20-45 °C (optimum growth at 37 °C), at pH 6.0-10.0 (optimum growth at pH 8.0) and in the presence of 0-2 % (w/v) NaCl (optimum growth with 0 % NaCl).
32375947	11	41	theme	novel	1465:1469	arg1	species					1471:1477	a novel species	1463:1477	a novel species	1463:1477	On the basis of its phenotypic, chemotaxonomic and phylogenetic properties, strain 3 H-10T represents a novel species of the genus Bacillus, for which the name Bacillus aquiflavi sp.
32375947	9	42	theme	strain	1215:1220	arg1	3 H-10T					1222:1228	strain 3 H-10T	1215:1228	strain 3 H-10T	1215:1228	The average nucleotide identity (ANI) values between strain 3 H-10T and the three type strains mentioned above were 69.56, 70.19 and 70.67 %, respectively.
32375947	9	43	theme	nucleotide	1174:1183	arg1	ANI					1195:1197	ANI	1195:1197	ANI	1195:1197	The average nucleotide identity (ANI) values between strain 3 H-10T and the three type strains mentioned above were 69.56, 70.19 and 70.67 %, respectively.
32375947	9	43	theme	nucleotide	1174:1183	arg1	identity					1185:1192	The average nucleotide identity	1162:1192	The average nucleotide identity (ANI) values between strain 3 H-10T and the three type strains mentioned above	1162:1271	The average nucleotide identity (ANI) values between strain 3 H-10T and the three type strains mentioned above were 69.56, 70.19 and 70.67 %, respectively.
32375947	4	44	with	growth	597:602	arg1	NaCl					613:616	0 % NaCl	609:616	0 % NaCl	609:616	The isolate was able to grow at temperatures of 20-45 °C (optimum growth at 37 °C), at pH 6.0-10.0 (optimum growth at pH 8.0) and in the presence of 0-2 % (w/v) NaCl (optimum growth with 0 % NaCl).
32375947	2	45	theme	PR	332:333	arg1	China					335:339	PR China	332:339	Sichuan province (PR China)	314:340	A Gram-stain-positive, strictly aerobic and rod-shaped bacterium, designated as 3 H-10T, was isolated from a yellow water sample collected from the manufacturing process of strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China).
32375947	8	46	theme	16S	961:963	arg1	rRNA					965:968	16S rRNA	961:968	16S rRNA gene sequence	961:982	Comparisons of 16S rRNA gene sequence indicated that 3 H-10T was most closely related to Bacillus mesophilus SA4T (96.30 %), Bacillus ginsengihumi Gsoil 114T (96.27 %) and Bacillus shackletonii LMG 18435T (96.27 %).
32375947	6	47	theme	3 H-10T	754:760	arg1	lipids					744:749	The main polar lipids	729:749	The main polar lipids of 3 H-10T	729:760	The main polar lipids of 3 H-10T included diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and phosphatidylethanolamine (PE).
32375947	10	48	theme	G+C	1334:1336	arg1	content					1338:1344	The genomic DNA G+C content	1318:1344	The genomic DNA G+C content	1318:1344	The genomic DNA G+C content was 35.4 mol%.
32375947	10	48	theme	G+C	1334:1336	arg1	%					1358:1358	35.4 mol%	1350:1358	35.4 mol%	1350:1358	The genomic DNA G+C content was 35.4 mol%.
32375947	7	49	theme	major	928:932	arg1	 0					908:909	 0	908:909	 0 (60.7 %)	908:918	MK-7 was predominant menaquinone and iso-C15 : 0 (60.7 %) was the major fatty acid.
32375947	7	49	theme	major	928:932	arg1	acid					940:943	the major fatty acid	924:943	the major fatty acid	924:943	MK-7 was predominant menaquinone and iso-C15 : 0 (60.7 %) was the major fatty acid.
32375947	2	50	theme	flavor	273:278	arg1	baijiu					288:293	strong flavor Chinese baijiu	266:293	strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China)	266:340	A Gram-stain-positive, strictly aerobic and rod-shaped bacterium, designated as 3 H-10T, was isolated from a yellow water sample collected from the manufacturing process of strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China).
32375947	8	51	theme	gene	970:973	arg1	sequence					975:982	16S rRNA gene sequence	961:982	16S rRNA gene sequence	961:982	Comparisons of 16S rRNA gene sequence indicated that 3 H-10T was most closely related to Bacillus mesophilus SA4T (96.30 %), Bacillus ginsengihumi Gsoil 114T (96.27 %) and Bacillus shackletonii LMG 18435T (96.27 %).
32375947	8	52	theme	Bacillus	1118:1125	arg1	18435T					1144:1149	Bacillus shackletonii LMG 18435T	1118:1149	Bacillus shackletonii LMG 18435T (96.27 %)	1118:1159	Comparisons of 16S rRNA gene sequence indicated that 3 H-10T was most closely related to Bacillus mesophilus SA4T (96.30 %), Bacillus ginsengihumi Gsoil 114T (96.27 %) and Bacillus shackletonii LMG 18435T (96.27 %).
32375947	8	52	theme	Bacillus	1118:1125	arg1	%					1158:1158	96.27 %	1152:1158	96.27 %	1152:1158	Comparisons of 16S rRNA gene sequence indicated that 3 H-10T was most closely related to Bacillus mesophilus SA4T (96.30 %), Bacillus ginsengihumi Gsoil 114T (96.27 %) and Bacillus shackletonii LMG 18435T (96.27 %).
32375947	10	53	theme	genomic	1322:1328	arg1	content					1338:1344	The genomic DNA G+C content	1318:1344	The genomic DNA G+C content	1318:1344	The genomic DNA G+C content was 35.4 mol%.
32375947	10	53	theme	genomic	1322:1328	arg1	%					1358:1358	35.4 mol%	1350:1358	35.4 mol%	1350:1358	The genomic DNA G+C content was 35.4 mol%.
32375947	2	54	theme	rod-shaped	137:146	arg1	bacterium					148:156	A Gram-stain-positive, strictly aerobic and rod-shaped bacterium	93:156	A Gram-stain-positive, strictly aerobic and rod-shaped bacterium	93:156	A Gram-stain-positive, strictly aerobic and rod-shaped bacterium, designated as 3 H-10T, was isolated from a yellow water sample collected from the manufacturing process of strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China).
32375947	5	55	theme	diagnostic	706:715	arg1	acid					682:685	meso-diaminopimelic acid	662:685	meso-diaminopimelic acid (meso-DAP)	662:696	Ribose was the major cell-wall sugar, and meso-diaminopimelic acid (meso-DAP) was the diagnostic amino acid.
32375947	5	55	theme	diagnostic	706:715	arg1	acid					723:726	the diagnostic amino acid	702:726	the diagnostic amino acid	702:726	Ribose was the major cell-wall sugar, and meso-diaminopimelic acid (meso-DAP) was the diagnostic amino acid.
32375947	6	56	theme	main	733:736	arg1	lipids					744:749	The main polar lipids	729:749	The main polar lipids of 3 H-10T	729:760	The main polar lipids of 3 H-10T included diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and phosphatidylethanolamine (PE).
32375947	2	57	theme	Yibin	298:302	arg1	region					304:309	Yibin region	298:309	Yibin region of Sichuan province (PR China)	298:340	A Gram-stain-positive, strictly aerobic and rod-shaped bacterium, designated as 3 H-10T, was isolated from a yellow water sample collected from the manufacturing process of strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China).
32375947	2	58	theme	baijiu	288:293	arg1	process					255:261	the manufacturing process	237:261	the manufacturing process of strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China)	237:340	A Gram-stain-positive, strictly aerobic and rod-shaped bacterium, designated as 3 H-10T, was isolated from a yellow water sample collected from the manufacturing process of strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China).
32375947	11	59	theme	aquiflavi	1530:1538	arg1	sp					1540:1541	the name Bacillus aquiflavi sp	1512:1541	the name Bacillus aquiflavi sp	1512:1541	On the basis of its phenotypic, chemotaxonomic and phylogenetic properties, strain 3 H-10T represents a novel species of the genus Bacillus, for which the name Bacillus aquiflavi sp.
32375947	9	60	theme	type	1244:1247	arg1	strains					1249:1255	the three type strains	1234:1255	the three type strains mentioned above	1234:1271	The average nucleotide identity (ANI) values between strain 3 H-10T and the three type strains mentioned above were 69.56, 70.19 and 70.67 %, respectively.
32375947	8	61	theme	Bacillus	1071:1078	arg1	114T					1099:1102	Bacillus ginsengihumi Gsoil 114T	1071:1102	Bacillus ginsengihumi Gsoil 114T (96.27 %)	1071:1112	Comparisons of 16S rRNA gene sequence indicated that 3 H-10T was most closely related to Bacillus mesophilus SA4T (96.30 %), Bacillus ginsengihumi Gsoil 114T (96.27 %) and Bacillus shackletonii LMG 18435T (96.27 %).
32375947	8	61	theme	Bacillus	1071:1078	arg1	%					1111:1111	96.27 %	1105:1111	96.27 %	1105:1111	Comparisons of 16S rRNA gene sequence indicated that 3 H-10T was most closely related to Bacillus mesophilus SA4T (96.30 %), Bacillus ginsengihumi Gsoil 114T (96.27 %) and Bacillus shackletonii LMG 18435T (96.27 %).
32375947	2	62	theme	yellow	202:207	arg1	sample					215:220	a yellow water sample	200:220	a yellow water sample collected from the manufacturing process of strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China)	200:340	A Gram-stain-positive, strictly aerobic and rod-shaped bacterium, designated as 3 H-10T, was isolated from a yellow water sample collected from the manufacturing process of strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China).
32375947	8	63	theme	Bacillus	1035:1042	arg1	SA4T					1055:1058	Bacillus mesophilus SA4T	1035:1058	Bacillus mesophilus SA4T (96.30 %)	1035:1068	Comparisons of 16S rRNA gene sequence indicated that 3 H-10T was most closely related to Bacillus mesophilus SA4T (96.30 %), Bacillus ginsengihumi Gsoil 114T (96.27 %) and Bacillus shackletonii LMG 18435T (96.27 %).
32375947	8	63	theme	Bacillus	1035:1042	arg1	%					1067:1067	96.30 %	1061:1067	96.30 %	1061:1067	Comparisons of 16S rRNA gene sequence indicated that 3 H-10T was most closely related to Bacillus mesophilus SA4T (96.30 %), Bacillus ginsengihumi Gsoil 114T (96.27 %) and Bacillus shackletonii LMG 18435T (96.27 %).
32375947	8	64	theme	Gsoil	1093:1097	arg1	114T					1099:1102	Bacillus ginsengihumi Gsoil 114T	1071:1102	Bacillus ginsengihumi Gsoil 114T (96.27 %)	1071:1112	Comparisons of 16S rRNA gene sequence indicated that 3 H-10T was most closely related to Bacillus mesophilus SA4T (96.30 %), Bacillus ginsengihumi Gsoil 114T (96.27 %) and Bacillus shackletonii LMG 18435T (96.27 %).
32375947	8	64	theme	Gsoil	1093:1097	arg1	%					1111:1111	96.27 %	1105:1111	96.27 %	1105:1111	Comparisons of 16S rRNA gene sequence indicated that 3 H-10T was most closely related to Bacillus mesophilus SA4T (96.30 %), Bacillus ginsengihumi Gsoil 114T (96.27 %) and Bacillus shackletonii LMG 18435T (96.27 %).
32375947	7	65	theme	predominant	871:881	arg1	menaquinone					883:893	predominant menaquinone	871:893	predominant menaquinone	871:893	MK-7 was predominant menaquinone and iso-C15 : 0 (60.7 %) was the major fatty acid.
32375947	2	66	theme	manufacturing	241:253	arg1	process					255:261	the manufacturing process	237:261	the manufacturing process of strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China)	237:340	A Gram-stain-positive, strictly aerobic and rod-shaped bacterium, designated as 3 H-10T, was isolated from a yellow water sample collected from the manufacturing process of strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China).
32375947	2	67	from	process	255:261	arg1	region					304:309	Yibin region	298:309	Yibin region of Sichuan province (PR China)	298:340	A Gram-stain-positive, strictly aerobic and rod-shaped bacterium, designated as 3 H-10T, was isolated from a yellow water sample collected from the manufacturing process of strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China).
32375947	1	68	theme	yellow	43:48	arg1	water					50:54	yellow water	43:54	yellow water of strongly flavored Chinese baijiu	43:90	nov., isolated from yellow water of strongly flavored Chinese baijiu.
32375947	13	69	theme	=CICC	1609:1613	arg1	33703T					1626:1631	=CICC 24755T=JCM 33703T	1609:1631	=CICC 24755T=JCM 33703T	1609:1631	The type strain is Bacillus aquiflavi 3 H-10T (=CICC 24755T=JCM 33703T).
32375947	13	69	theme	=CICC	1609:1613	arg1	3 H-10T					1600:1606	Bacillus aquiflavi 3 H-10T	1581:1606	Bacillus aquiflavi 3 H-10T (=CICC 24755T=JCM 33703T)	1581:1632	The type strain is Bacillus aquiflavi 3 H-10T (=CICC 24755T=JCM 33703T).
32375947	4	70	theme	20-45 °C	470:477	arg1	temperatures					454:465	temperatures	454:465	temperatures of 20-45 °C (optimum growth at 37 °C)	454:503	The isolate was able to grow at temperatures of 20-45 °C (optimum growth at 37 °C), at pH 6.0-10.0 (optimum growth at pH 8.0) and in the presence of 0-2 % (w/v) NaCl (optimum growth with 0 % NaCl).
32375947	11	71	theme	name	1516:1519	arg1	sp					1540:1541	the name Bacillus aquiflavi sp	1512:1541	the name Bacillus aquiflavi sp	1512:1541	On the basis of its phenotypic, chemotaxonomic and phylogenetic properties, strain 3 H-10T represents a novel species of the genus Bacillus, for which the name Bacillus aquiflavi sp.
32375947	4	72	dep	NaCl	583:586	arg1	growth					597:602	optimum growth	589:602	optimum growth with 0 % NaCl	589:616	The isolate was able to grow at temperatures of 20-45 °C (optimum growth at 37 °C), at pH 6.0-10.0 (optimum growth at pH 8.0) and in the presence of 0-2 % (w/v) NaCl (optimum growth with 0 % NaCl).
32375947	13	73	theme	Bacillus	1581:1588	arg1	strain					1571:1576	The type strain	1562:1576	The type strain	1562:1576	The type strain is Bacillus aquiflavi 3 H-10T (=CICC 24755T=JCM 33703T).
32375947	13	73	theme	Bacillus	1581:1588	arg1	33703T					1626:1631	=CICC 24755T=JCM 33703T	1609:1631	=CICC 24755T=JCM 33703T	1609:1631	The type strain is Bacillus aquiflavi 3 H-10T (=CICC 24755T=JCM 33703T).
32375947	13	73	theme	Bacillus	1581:1588	arg1	3 H-10T					1600:1606	Bacillus aquiflavi 3 H-10T	1581:1606	Bacillus aquiflavi 3 H-10T (=CICC 24755T=JCM 33703T)	1581:1632	The type strain is Bacillus aquiflavi 3 H-10T (=CICC 24755T=JCM 33703T).
32375947	2	74	attach	isolated	186:193	arg2	bacterium					148:156	A Gram-stain-positive, strictly aerobic and rod-shaped bacterium	93:156	A Gram-stain-positive, strictly aerobic and rod-shaped bacterium	93:156	A Gram-stain-positive, strictly aerobic and rod-shaped bacterium, designated as 3 H-10T, was isolated from a yellow water sample collected from the manufacturing process of strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China).
32375947	2	74	attach	isolated	186:193	arg1	sample					215:220	a yellow water sample	200:220	a yellow water sample collected from the manufacturing process of strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China)	200:340	A Gram-stain-positive, strictly aerobic and rod-shaped bacterium, designated as 3 H-10T, was isolated from a yellow water sample collected from the manufacturing process of strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China).
32375947	3	75	theme	swollen	403:409	arg1	sporangia					411:419	swollen sporangia	403:419	swollen sporangia	403:419	Oval endospores were formed at the subtermini of cells with swollen sporangia.
32375947	0	76	theme	aquiflavi	9:17	arg1	sp					19:20	Bacillus aquiflavi sp	0:20	Bacillus aquiflavi sp.	0:21	Bacillus aquiflavi sp.
32375947	1	77	theme	flavored	68:75	arg1	baijiu					85:90	strongly flavored Chinese baijiu	59:90	strongly flavored Chinese baijiu	59:90	nov., isolated from yellow water of strongly flavored Chinese baijiu.
32375947	4	78	theme	0 	609:610	arg1	%					611:611	%	611:611	%	611:611	The isolate was able to grow at temperatures of 20-45 °C (optimum growth at 37 °C), at pH 6.0-10.0 (optimum growth at pH 8.0) and in the presence of 0-2 % (w/v) NaCl (optimum growth with 0 % NaCl).
32375947	3	79	theme	cells	392:396	arg1	subtermini					378:387	the subtermini	374:387	the subtermini of cells with swollen sporangia	374:419	Oval endospores were formed at the subtermini of cells with swollen sporangia.
32375947	1	80	theme	baijiu	85:90	arg1	water					50:54	yellow water	43:54	yellow water of strongly flavored Chinese baijiu	43:90	nov., isolated from yellow water of strongly flavored Chinese baijiu.
32375947	3	81	theme	Oval	343:346	arg1	endospores					348:357	Oval endospores	343:357	Oval endospores	343:357	Oval endospores were formed at the subtermini of cells with swollen sporangia.
32375947	8	82	dep	Bacillus	1035:1042	arg1	mesophilus					1044:1053	mesophilus	1044:1053	mesophilus	1044:1053	Comparisons of 16S rRNA gene sequence indicated that 3 H-10T was most closely related to Bacillus mesophilus SA4T (96.30 %), Bacillus ginsengihumi Gsoil 114T (96.27 %) and Bacillus shackletonii LMG 18435T (96.27 %).
32375947	5	83	theme	major	635:639	arg1	sugar					651:655	the major cell-wall sugar	631:655	the major cell-wall sugar	631:655	Ribose was the major cell-wall sugar, and meso-diaminopimelic acid (meso-DAP) was the diagnostic amino acid.
32375947	5	83	theme	major	635:639	arg1	Ribose					620:625	Ribose	620:625	Ribose	620:625	Ribose was the major cell-wall sugar, and meso-diaminopimelic acid (meso-DAP) was the diagnostic amino acid.
32375947	8	84	dep	Bacillus	1118:1125	arg1	shackletonii					1127:1138	shackletonii	1127:1138	shackletonii	1127:1138	Comparisons of 16S rRNA gene sequence indicated that 3 H-10T was most closely related to Bacillus mesophilus SA4T (96.30 %), Bacillus ginsengihumi Gsoil 114T (96.27 %) and Bacillus shackletonii LMG 18435T (96.27 %).
32375947	2	85	dep	Gram-stain-positive	95:113	arg1	aerobic					125:131	aerobic	125:131	aerobic	125:131	A Gram-stain-positive, strictly aerobic and rod-shaped bacterium, designated as 3 H-10T, was isolated from a yellow water sample collected from the manufacturing process of strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China).
32375947	4	86	theme	at	495:496	arg1	37 °C					498:502	optimum growth at 37 °C	480:502	optimum growth at 37 °C	480:502	The isolate was able to grow at temperatures of 20-45 °C (optimum growth at 37 °C), at pH 6.0-10.0 (optimum growth at pH 8.0) and in the presence of 0-2 % (w/v) NaCl (optimum growth with 0 % NaCl).
32375947	4	86	theme	at	495:496	arg1	20-45 °C					470:477	20-45 °C	470:477	20-45 °C (optimum growth at 37 °C)	470:503	The isolate was able to grow at temperatures of 20-45 °C (optimum growth at 37 °C), at pH 6.0-10.0 (optimum growth at pH 8.0) and in the presence of 0-2 % (w/v) NaCl (optimum growth with 0 % NaCl).
32375947	2	87	theme	Sichuan	314:320	arg1	province					322:329	Sichuan province	314:329	Sichuan province (PR China)	314:340	A Gram-stain-positive, strictly aerobic and rod-shaped bacterium, designated as 3 H-10T, was isolated from a yellow water sample collected from the manufacturing process of strong flavor Chinese baijiu in Yibin region of Sichuan province (PR China).
32375947	11	88	dep	properties	1425:1434	arg1	the					1364:1366	the	1364:1366	the	1364:1366	On the basis of its phenotypic, chemotaxonomic and phylogenetic properties, strain 3 H-10T represents a novel species of the genus Bacillus, for which the name Bacillus aquiflavi sp.
32375947	11	88	dep	properties	1425:1434	arg1	basis					1368:1372	basis	1368:1372	basis	1368:1372	On the basis of its phenotypic, chemotaxonomic and phylogenetic properties, strain 3 H-10T represents a novel species of the genus Bacillus, for which the name Bacillus aquiflavi sp.
32375947	4	89	theme	at	537:538	arg1	pH					509:510	pH 6.0-10.0	509:519	pH 6.0-10.0 (optimum growth at pH 8.0)	509:546	The isolate was able to grow at temperatures of 20-45 °C (optimum growth at 37 °C), at pH 6.0-10.0 (optimum growth at pH 8.0) and in the presence of 0-2 % (w/v) NaCl (optimum growth with 0 % NaCl).
32375947	4	89	theme	at	537:538	arg1	pH					540:541	optimum growth at pH 8.0	522:545	optimum growth at pH 8.0	522:545	The isolate was able to grow at temperatures of 20-45 °C (optimum growth at 37 °C), at pH 6.0-10.0 (optimum growth at pH 8.0) and in the presence of 0-2 % (w/v) NaCl (optimum growth with 0 % NaCl).
32375947	9	90	theme	average	1166:1172	arg1	ANI					1195:1197	ANI	1195:1197	ANI	1195:1197	The average nucleotide identity (ANI) values between strain 3 H-10T and the three type strains mentioned above were 69.56, 70.19 and 70.67 %, respectively.
32375947	9	90	theme	average	1166:1172	arg1	identity					1185:1192	The average nucleotide identity	1162:1192	The average nucleotide identity (ANI) values between strain 3 H-10T and the three type strains mentioned above	1162:1271	The average nucleotide identity (ANI) values between strain 3 H-10T and the three type strains mentioned above were 69.56, 70.19 and 70.67 %, respectively.
32375947	11	91	theme	phenotypic	1381:1390	arg1	properties					1425:1434	its phenotypic, chemotaxonomic and phylogenetic properties	1377:1434	its phenotypic, chemotaxonomic and phylogenetic properties	1377:1434	On the basis of its phenotypic, chemotaxonomic and phylogenetic properties, strain 3 H-10T represents a novel species of the genus Bacillus, for which the name Bacillus aquiflavi sp.
32375947	9	92	theme	identity	1185:1192	arg1	values					1200:1205	The average nucleotide identity (ANI) values	1162:1205	The average nucleotide identity (ANI) values between strain 3 H-10T and the three type strains mentioned above	1162:1271	The average nucleotide identity (ANI) values between strain 3 H-10T and the three type strains mentioned above were 69.56, 70.19 and 70.67 %, respectively.
34834067	1	0	theme	colorant	341:348	arg1	stabilizer					359:368	a colorant or jelly stabilizer	339:368	a colorant or jelly stabilizer	339:368	Curcumin (CCM) is a natural hydrophobic polyphenol known for its numerous applications in the food industry as a colorant or jelly stabilizer, and in the pharmaceutical industry due to its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties.
34834067	7	1	theme	driving	1589:1595	arg1	force					1597:1601	the main driving force	1580:1601	the main driving force of drug release from the composite cryogels	1580:1645	Fitting Higuchi, Korsmeyer-Peppas, and first order kinetic models on the CCM release profiles indicated the diffusion as the main driving force of drug release from the composite cryogels.
34834067	4	2	theme	semi-IPN	1085:1092	arg1	cryogel					1094:1100	semi-IPN cryogel	1085:1100	semi-IPN cryogel with monochlorotriazinyl-β-cyclodextrin (MCT-β-CD) led to IPN cryogel	1085:1170	All s-IPN cryogels were characterized by SEM, EDX, FTIR, and swelling at equilibrium as a function of pH. Functionalization of semi-IPN cryogel with monochlorotriazinyl-β-cyclodextrin (MCT-β-CD) led to IPN cryogel.
34834067	7	3	theme	drug	1606:1609	arg1	release					1611:1617	drug release	1606:1617	drug release from the composite cryogels	1606:1645	Fitting Higuchi, Korsmeyer-Peppas, and first order kinetic models on the CCM release profiles indicated the diffusion as the main driving force of drug release from the composite cryogels.
34834067	1	4	theme	hydrophobic	256:266	arg1	polyphenol					268:277	a natural hydrophobic polyphenol	246:277	a natural hydrophobic polyphenol known for its numerous applications in the food industry as a colorant or jelly stabilizer, and in the pharmaceutical industry due to its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties	246:505	Curcumin (CCM) is a natural hydrophobic polyphenol known for its numerous applications in the food industry as a colorant or jelly stabilizer, and in the pharmaceutical industry due to its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties.
34834067	1	4	theme	hydrophobic	256:266	arg1	Curcumin					228:235	Curcumin	228:235	Curcumin (CCM)	228:241	Curcumin (CCM) is a natural hydrophobic polyphenol known for its numerous applications in the food industry as a colorant or jelly stabilizer, and in the pharmaceutical industry due to its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties.
34834067	3	5	theme	degradation	686:696	arg1	s-IPNs					802:807	s-IPNs	802:807	s-IPNs	802:807	To enhance the bioavailability of CCM, and to protect it against the external degradation agents, a novel strategy, which consists in the preparation of semi-interpenetrating polymer networks, (s-IPNs) based on poly(N,N-dimethylaminoethyl methacrylate) entrapped in poly(acrylamide) networks, by a cryogelation technique, was developed in this work.
34834067	3	5	theme	degradation	686:696	arg1	strategy					714:721	a novel strategy	706:721	a novel strategy	706:721	To enhance the bioavailability of CCM, and to protect it against the external degradation agents, a novel strategy, which consists in the preparation of semi-interpenetrating polymer networks, (s-IPNs) based on poly(N,N-dimethylaminoethyl methacrylate) entrapped in poly(acrylamide) networks, by a cryogelation technique, was developed in this work.
34834067	3	5	theme	degradation	686:696	arg1	agents					698:703	the external degradation agents	673:703	the external degradation agents	673:703	To enhance the bioavailability of CCM, and to protect it against the external degradation agents, a novel strategy, which consists in the preparation of semi-interpenetrating polymer networks, (s-IPNs) based on poly(N,N-dimethylaminoethyl methacrylate) entrapped in poly(acrylamide) networks, by a cryogelation technique, was developed in this work.
34834067	0	6	theme	Release	198:204	arg1	Kinetics					206:213	Release Kinetics	198:213	Release Kinetics	198:213	Preparation and Characterization of Semi-IPN Cryogels Based on Polyacrylamide and Poly(N,N-dimethylaminoethyl methacrylate); Functionalization of Carrier with Monochlorotriazinyl-β-cyclodextrin and Release Kinetics of Curcumin.
34834067	1	7	theme	antibacterial	436:448	arg1	properties					496:505	its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties	413:505	its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties	413:505	Curcumin (CCM) is a natural hydrophobic polyphenol known for its numerous applications in the food industry as a colorant or jelly stabilizer, and in the pharmaceutical industry due to its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties.
34834067	5	8	theme	CCM	1196:1198	arg1	profile					1185:1191	The release profile	1173:1191	The release profile of CCM from the composite cryogels	1173:1226	The release profile of CCM from the composite cryogels was investigated at 37 °C, in pH 3.
34834067	4	9	with	cryogel	1094:1100	arg1	monochlorotriazinyl-β-cyclodextrin					1107:1140	monochlorotriazinyl-β-cyclodextrin	1107:1140	monochlorotriazinyl-β-cyclodextrin (MCT-β-CD) led to IPN cryogel	1107:1170	All s-IPN cryogels were characterized by SEM, EDX, FTIR, and swelling at equilibrium as a function of pH. Functionalization of semi-IPN cryogel with monochlorotriazinyl-β-cyclodextrin (MCT-β-CD) led to IPN cryogel.
34834067	4	9	with	cryogel	1094:1100	arg1	MCT-β-CD					1143:1150	MCT-β-CD	1143:1150	MCT-β-CD	1143:1150	All s-IPN cryogels were characterized by SEM, EDX, FTIR, and swelling at equilibrium as a function of pH. Functionalization of semi-IPN cryogel with monochlorotriazinyl-β-cyclodextrin (MCT-β-CD) led to IPN cryogel.
34834067	1	10	theme	jelly	353:357	arg1	stabilizer					359:368	a colorant or jelly stabilizer	339:368	a colorant or jelly stabilizer	339:368	Curcumin (CCM) is a natural hydrophobic polyphenol known for its numerous applications in the food industry as a colorant or jelly stabilizer, and in the pharmaceutical industry due to its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties.
34834067	0	11	theme	Curcumin	218:225	arg1	Monochlorotriazinyl-β-cyclodextrin					159:192	Monochlorotriazinyl-β-cyclodextrin	159:192	Monochlorotriazinyl-β-cyclodextrin	159:192	Preparation and Characterization of Semi-IPN Cryogels Based on Polyacrylamide and Poly(N,N-dimethylaminoethyl methacrylate); Functionalization of Carrier with Monochlorotriazinyl-β-cyclodextrin and Release Kinetics of Curcumin.
34834067	0	11	theme	Curcumin	218:225	arg1	Kinetics					206:213	Release Kinetics	198:213	Release Kinetics	198:213	Preparation and Characterization of Semi-IPN Cryogels Based on Polyacrylamide and Poly(N,N-dimethylaminoethyl methacrylate); Functionalization of Carrier with Monochlorotriazinyl-β-cyclodextrin and Release Kinetics of Curcumin.
34834067	3	12	theme	N-dimethylaminoethyl	826:845	arg1	methacrylate					847:858	N-dimethylaminoethyl methacrylate	826:858	N-dimethylaminoethyl methacrylate	826:858	To enhance the bioavailability of CCM, and to protect it against the external degradation agents, a novel strategy, which consists in the preparation of semi-interpenetrating polymer networks, (s-IPNs) based on poly(N,N-dimethylaminoethyl methacrylate) entrapped in poly(acrylamide) networks, by a cryogelation technique, was developed in this work.
34834067	5	13	theme	release	1177:1183	arg1	profile					1185:1191	The release profile	1173:1191	The release profile of CCM from the composite cryogels	1173:1226	The release profile of CCM from the composite cryogels was investigated at 37 °C, in pH 3.
34834067	1	14	theme	antioxidant	451:461	arg1	properties					496:505	its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties	413:505	its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties	413:505	Curcumin (CCM) is a natural hydrophobic polyphenol known for its numerous applications in the food industry as a colorant or jelly stabilizer, and in the pharmaceutical industry due to its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties.
34834067	7	15	from	force	1597:1601	arg1	cryogels					1638:1645	the composite cryogels	1624:1645	the composite cryogels	1624:1645	Fitting Higuchi, Korsmeyer-Peppas, and first order kinetic models on the CCM release profiles indicated the diffusion as the main driving force of drug release from the composite cryogels.
34834067	3	16	theme	poly	874:877	arg1	networks					891:898	poly(acrylamide) networks	874:898	poly(acrylamide) networks	874:898	To enhance the bioavailability of CCM, and to protect it against the external degradation agents, a novel strategy, which consists in the preparation of semi-interpenetrating polymer networks, (s-IPNs) based on poly(N,N-dimethylaminoethyl methacrylate) entrapped in poly(acrylamide) networks, by a cryogelation technique, was developed in this work.
34834067	2	17	from	solubility	569:578	arg1	water					583:587	water	583:587	water	583:587	However, the large application of CCM is limited by its poor solubility in water and low stability.
34834067	2	17	from	solubility	569:578	arg1	stability					597:605	low stability	593:605	low stability	593:605	However, the large application of CCM is limited by its poor solubility in water and low stability.
34834067	2	18	theme	large	521:525	arg1	application					527:537	the large application	517:537	the large application of CCM	517:544	However, the large application of CCM is limited by its poor solubility in water and low stability.
34834067	3	19	theme	novel	708:712	arg1	strategy					714:721	a novel strategy	706:721	a novel strategy	706:721	To enhance the bioavailability of CCM, and to protect it against the external degradation agents, a novel strategy, which consists in the preparation of semi-interpenetrating polymer networks, (s-IPNs) based on poly(N,N-dimethylaminoethyl methacrylate) entrapped in poly(acrylamide) networks, by a cryogelation technique, was developed in this work.
34834067	3	19	theme	novel	708:712	arg1	agents					698:703	the external degradation agents	673:703	the external degradation agents	673:703	To enhance the bioavailability of CCM, and to protect it against the external degradation agents, a novel strategy, which consists in the preparation of semi-interpenetrating polymer networks, (s-IPNs) based on poly(N,N-dimethylaminoethyl methacrylate) entrapped in poly(acrylamide) networks, by a cryogelation technique, was developed in this work.
34834067	1	20	from	applications	302:313	arg1	industry					327:334	the food industry	318:334	the food industry	318:334	Curcumin (CCM) is a natural hydrophobic polyphenol known for its numerous applications in the food industry as a colorant or jelly stabilizer, and in the pharmaceutical industry due to its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties.
34834067	3	21	dep	N	824:824	arg1	methacrylate					847:858	N-dimethylaminoethyl methacrylate	826:858	N-dimethylaminoethyl methacrylate	826:858	To enhance the bioavailability of CCM, and to protect it against the external degradation agents, a novel strategy, which consists in the preparation of semi-interpenetrating polymer networks, (s-IPNs) based on poly(N,N-dimethylaminoethyl methacrylate) entrapped in poly(acrylamide) networks, by a cryogelation technique, was developed in this work.
34834067	6	22	theme	molar	1436:1440	arg1	degree					1408:1413	the cross-linking degree	1390:1413	the cross-linking degree	1390:1413	It was found that the cumulative release increased with the increase of the carrier hydrophobicity, as a result of increasing the cross-linking degree, the content and the molar mass of PDMAEMA.
34834067	6	22	theme	molar	1436:1440	arg1	mass					1442:1445	the molar mass	1432:1445	the molar mass of PDMAEMA	1432:1456	It was found that the cumulative release increased with the increase of the carrier hydrophobicity, as a result of increasing the cross-linking degree, the content and the molar mass of PDMAEMA.
34834067	0	23	theme	N-dimethylaminoethyl	89:108	arg1	N					87:87	N	87:87	N	87:87	Preparation and Characterization of Semi-IPN Cryogels Based on Polyacrylamide and Poly(N,N-dimethylaminoethyl methacrylate); Functionalization of Carrier with Monochlorotriazinyl-β-cyclodextrin and Release Kinetics of Curcumin.
34834067	0	23	theme	N-dimethylaminoethyl	89:108	arg1	methacrylate					110:121	N-dimethylaminoethyl methacrylate	89:121	N-dimethylaminoethyl methacrylate	89:121	Preparation and Characterization of Semi-IPN Cryogels Based on Polyacrylamide and Poly(N,N-dimethylaminoethyl methacrylate); Functionalization of Carrier with Monochlorotriazinyl-β-cyclodextrin and Release Kinetics of Curcumin.
34834067	1	24	theme	anti-cancer	464:474	arg1	properties					496:505	its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties	413:505	its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties	413:505	Curcumin (CCM) is a natural hydrophobic polyphenol known for its numerous applications in the food industry as a colorant or jelly stabilizer, and in the pharmaceutical industry due to its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties.
34834067	6	25	theme	carrier	1340:1346	arg1	hydrophobicity					1348:1361	the carrier hydrophobicity	1336:1361	the carrier hydrophobicity	1336:1361	It was found that the cumulative release increased with the increase of the carrier hydrophobicity, as a result of increasing the cross-linking degree, the content and the molar mass of PDMAEMA.
34834067	4	26	theme	pH.	1060:1062	arg1	Functionalization					1064:1080	pH. Functionalization	1060:1080	pH. Functionalization of semi-IPN cryogel with monochlorotriazinyl-β-cyclodextrin (MCT-β-CD) led to IPN cryogel	1060:1170	All s-IPN cryogels were characterized by SEM, EDX, FTIR, and swelling at equilibrium as a function of pH. Functionalization of semi-IPN cryogel with monochlorotriazinyl-β-cyclodextrin (MCT-β-CD) led to IPN cryogel.
34834067	6	27	theme	hydrophobicity	1348:1361	arg1	increase					1324:1331	the increase	1320:1331	the increase of the carrier hydrophobicity	1320:1361	It was found that the cumulative release increased with the increase of the carrier hydrophobicity, as a result of increasing the cross-linking degree, the content and the molar mass of PDMAEMA.
34834067	7	28	theme	composite	1628:1636	arg1	cryogels					1638:1645	the composite cryogels	1624:1645	the composite cryogels	1624:1645	Fitting Higuchi, Korsmeyer-Peppas, and first order kinetic models on the CCM release profiles indicated the diffusion as the main driving force of drug release from the composite cryogels.
34834067	5	29	from	cryogels	1219:1226	arg1	profile					1185:1191	The release profile	1173:1191	The release profile of CCM from the composite cryogels	1173:1226	The release profile of CCM from the composite cryogels was investigated at 37 °C, in pH 3.
34834067	4	30	theme	s-IPN	962:966	arg1	cryogels					968:975	All s-IPN cryogels	958:975	All s-IPN cryogels	958:975	All s-IPN cryogels were characterized by SEM, EDX, FTIR, and swelling at equilibrium as a function of pH. Functionalization of semi-IPN cryogel with monochlorotriazinyl-β-cyclodextrin (MCT-β-CD) led to IPN cryogel.
34834067	4	30	theme	s-IPN	962:966	arg1	function					1048:1055	a function	1046:1055	a function of pH. Functionalization of semi-IPN cryogel with monochlorotriazinyl-β-cyclodextrin (MCT-β-CD) led to IPN cryogel	1046:1170	All s-IPN cryogels were characterized by SEM, EDX, FTIR, and swelling at equilibrium as a function of pH. Functionalization of semi-IPN cryogel with monochlorotriazinyl-β-cyclodextrin (MCT-β-CD) led to IPN cryogel.
34834067	4	31	theme	IPN	1160:1162	arg1	cryogel					1164:1170	IPN cryogel	1160:1170	IPN cryogel	1160:1170	All s-IPN cryogels were characterized by SEM, EDX, FTIR, and swelling at equilibrium as a function of pH. Functionalization of semi-IPN cryogel with monochlorotriazinyl-β-cyclodextrin (MCT-β-CD) led to IPN cryogel.
34834067	0	32	dep	Poly	82:85	arg1	N					87:87	N	87:87	N	87:87	Preparation and Characterization of Semi-IPN Cryogels Based on Polyacrylamide and Poly(N,N-dimethylaminoethyl methacrylate); Functionalization of Carrier with Monochlorotriazinyl-β-cyclodextrin and Release Kinetics of Curcumin.
34834067	0	32	dep	Poly	82:85	arg1	methacrylate					110:121	N-dimethylaminoethyl methacrylate	89:121	N-dimethylaminoethyl methacrylate	89:121	Preparation and Characterization of Semi-IPN Cryogels Based on Polyacrylamide and Poly(N,N-dimethylaminoethyl methacrylate); Functionalization of Carrier with Monochlorotriazinyl-β-cyclodextrin and Release Kinetics of Curcumin.
34834067	2	33	theme	low	593:595	arg1	stability					597:605	low stability	593:605	low stability	593:605	However, the large application of CCM is limited by its poor solubility in water and low stability.
34834067	1	34	theme	numerous	293:300	arg1	applications					302:313	its numerous applications	289:313	its numerous applications in the food industry	289:334	Curcumin (CCM) is a natural hydrophobic polyphenol known for its numerous applications in the food industry as a colorant or jelly stabilizer, and in the pharmaceutical industry due to its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties.
34834067	1	35	theme	anti-Alzheimer	481:494	arg1	properties					496:505	its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties	413:505	its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties	413:505	Curcumin (CCM) is a natural hydrophobic polyphenol known for its numerous applications in the food industry as a colorant or jelly stabilizer, and in the pharmaceutical industry due to its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties.
34834067	3	36	theme	CCM	642:644	arg1	bioavailability					623:637	the bioavailability	619:637	the bioavailability of CCM	619:644	To enhance the bioavailability of CCM, and to protect it against the external degradation agents, a novel strategy, which consists in the preparation of semi-interpenetrating polymer networks, (s-IPNs) based on poly(N,N-dimethylaminoethyl methacrylate) entrapped in poly(acrylamide) networks, by a cryogelation technique, was developed in this work.
34834067	7	37	theme	release	1536:1542	arg1	profiles					1544:1551	the CCM release profiles	1528:1551	the CCM release profiles	1528:1551	Fitting Higuchi, Korsmeyer-Peppas, and first order kinetic models on the CCM release profiles indicated the diffusion as the main driving force of drug release from the composite cryogels.
34834067	0	38	theme	Cryogels	45:52	arg1	Characterization					16:31	Characterization	16:31	Characterization	16:31	Preparation and Characterization of Semi-IPN Cryogels Based on Polyacrylamide and Poly(N,N-dimethylaminoethyl methacrylate); Functionalization of Carrier with Monochlorotriazinyl-β-cyclodextrin and Release Kinetics of Curcumin.
34834067	0	38	theme	Cryogels	45:52	arg1	Preparation					0:10	Preparation	0:10	Preparation	0:10	Preparation and Characterization of Semi-IPN Cryogels Based on Polyacrylamide and Poly(N,N-dimethylaminoethyl methacrylate); Functionalization of Carrier with Monochlorotriazinyl-β-cyclodextrin and Release Kinetics of Curcumin.
34834067	7	39	theme	main	1584:1587	arg1	force					1597:1601	the main driving force	1580:1601	the main driving force of drug release from the composite cryogels	1580:1645	Fitting Higuchi, Korsmeyer-Peppas, and first order kinetic models on the CCM release profiles indicated the diffusion as the main driving force of drug release from the composite cryogels.
34834067	7	40	theme	CCM	1532:1534	arg1	profiles					1544:1551	the CCM release profiles	1528:1551	the CCM release profiles	1528:1551	Fitting Higuchi, Korsmeyer-Peppas, and first order kinetic models on the CCM release profiles indicated the diffusion as the main driving force of drug release from the composite cryogels.
34834067	1	41	theme	pharmaceutical	382:395	arg1	industry					397:404	the pharmaceutical industry	378:404	the pharmaceutical industry due to its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties	378:505	Curcumin (CCM) is a natural hydrophobic polyphenol known for its numerous applications in the food industry as a colorant or jelly stabilizer, and in the pharmaceutical industry due to its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties.
34834067	0	42	theme	Semi-IPN	36:43	arg1	Cryogels					45:52	Semi-IPN Cryogels	36:52	Semi-IPN Cryogels	36:52	Preparation and Characterization of Semi-IPN Cryogels Based on Polyacrylamide and Poly(N,N-dimethylaminoethyl methacrylate); Functionalization of Carrier with Monochlorotriazinyl-β-cyclodextrin and Release Kinetics of Curcumin.
34834067	7	43	theme	order	1504:1508	arg1	models					1518:1523	Higuchi, Korsmeyer-Peppas, and first order kinetic models	1467:1523	Higuchi, Korsmeyer-Peppas, and first order kinetic models on the CCM release profiles	1467:1551	Fitting Higuchi, Korsmeyer-Peppas, and first order kinetic models on the CCM release profiles indicated the diffusion as the main driving force of drug release from the composite cryogels.
34834067	2	44	theme	CCM	542:544	arg1	application					527:537	the large application	517:537	the large application of CCM	517:544	However, the large application of CCM is limited by its poor solubility in water and low stability.
34834067	6	45	theme	PDMAEMA	1450:1456	arg1	degree					1408:1413	the cross-linking degree	1390:1413	the cross-linking degree	1390:1413	It was found that the cumulative release increased with the increase of the carrier hydrophobicity, as a result of increasing the cross-linking degree, the content and the molar mass of PDMAEMA.
34834067	6	45	theme	PDMAEMA	1450:1456	arg1	content					1420:1426	the content	1416:1426	the content	1416:1426	It was found that the cumulative release increased with the increase of the carrier hydrophobicity, as a result of increasing the cross-linking degree, the content and the molar mass of PDMAEMA.
34834067	6	45	theme	PDMAEMA	1450:1456	arg1	mass					1442:1445	the molar mass	1432:1445	the molar mass of PDMAEMA	1432:1456	It was found that the cumulative release increased with the increase of the carrier hydrophobicity, as a result of increasing the cross-linking degree, the content and the molar mass of PDMAEMA.
34834067	0	46	dep	Based	54:58	arg1	Functionalization					125:141	Functionalization	125:141	Functionalization of Carrier with Monochlorotriazinyl-β-cyclodextrin and Release Kinetics of Curcumin	125:225	Preparation and Characterization of Semi-IPN Cryogels Based on Polyacrylamide and Poly(N,N-dimethylaminoethyl methacrylate); Functionalization of Carrier with Monochlorotriazinyl-β-cyclodextrin and Release Kinetics of Curcumin.
34834067	3	47	theme	cryogelation	906:917	arg1	technique					919:927	a cryogelation technique	904:927	a cryogelation technique	904:927	To enhance the bioavailability of CCM, and to protect it against the external degradation agents, a novel strategy, which consists in the preparation of semi-interpenetrating polymer networks, (s-IPNs) based on poly(N,N-dimethylaminoethyl methacrylate) entrapped in poly(acrylamide) networks, by a cryogelation technique, was developed in this work.
34834067	1	48	theme	food	322:325	arg1	industry					327:334	the food industry	318:334	the food industry	318:334	Curcumin (CCM) is a natural hydrophobic polyphenol known for its numerous applications in the food industry as a colorant or jelly stabilizer, and in the pharmaceutical industry due to its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties.
34834067	7	49	from	models	1518:1523	arg1	profiles					1544:1551	the CCM release profiles	1528:1551	the CCM release profiles	1528:1551	Fitting Higuchi, Korsmeyer-Peppas, and first order kinetic models on the CCM release profiles indicated the diffusion as the main driving force of drug release from the composite cryogels.
34834067	1	50	theme	due	406:408	arg1	industry					397:404	the pharmaceutical industry	378:404	the pharmaceutical industry due to its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties	378:505	Curcumin (CCM) is a natural hydrophobic polyphenol known for its numerous applications in the food industry as a colorant or jelly stabilizer, and in the pharmaceutical industry due to its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties.
34834067	7	51	from	cryogels	1638:1645	arg1	force					1597:1601	the main driving force	1580:1601	the main driving force of drug release from the composite cryogels	1580:1645	Fitting Higuchi, Korsmeyer-Peppas, and first order kinetic models on the CCM release profiles indicated the diffusion as the main driving force of drug release from the composite cryogels.
34834067	7	51	from	cryogels	1638:1645	arg1	release					1611:1617	drug release	1606:1617	drug release from the composite cryogels	1606:1645	Fitting Higuchi, Korsmeyer-Peppas, and first order kinetic models on the CCM release profiles indicated the diffusion as the main driving force of drug release from the composite cryogels.
34834067	0	52	theme	Carrier	146:152	arg1	Functionalization					125:141	Functionalization	125:141	Functionalization of Carrier with Monochlorotriazinyl-β-cyclodextrin and Release Kinetics of Curcumin	125:225	Preparation and Characterization of Semi-IPN Cryogels Based on Polyacrylamide and Poly(N,N-dimethylaminoethyl methacrylate); Functionalization of Carrier with Monochlorotriazinyl-β-cyclodextrin and Release Kinetics of Curcumin.
34834067	6	53	theme	cross-linking	1394:1406	arg1	degree					1408:1413	the cross-linking degree	1390:1413	the cross-linking degree	1390:1413	It was found that the cumulative release increased with the increase of the carrier hydrophobicity, as a result of increasing the cross-linking degree, the content and the molar mass of PDMAEMA.
34834067	6	53	theme	cross-linking	1394:1406	arg1	content					1420:1426	the content	1416:1426	the content	1416:1426	It was found that the cumulative release increased with the increase of the carrier hydrophobicity, as a result of increasing the cross-linking degree, the content and the molar mass of PDMAEMA.
34834067	6	53	theme	cross-linking	1394:1406	arg1	mass					1442:1445	the molar mass	1432:1445	the molar mass of PDMAEMA	1432:1456	It was found that the cumulative release increased with the increase of the carrier hydrophobicity, as a result of increasing the cross-linking degree, the content and the molar mass of PDMAEMA.
34834067	7	54	theme	first	1498:1502	arg1	order					1504:1508	first order	1498:1508	first order	1498:1508	Fitting Higuchi, Korsmeyer-Peppas, and first order kinetic models on the CCM release profiles indicated the diffusion as the main driving force of drug release from the composite cryogels.
34834067	2	55	theme	poor	564:567	arg1	solubility					569:578	its poor solubility	560:578	its poor solubility in water and low stability	560:605	However, the large application of CCM is limited by its poor solubility in water and low stability.
34834067	0	56	with	Functionalization	125:141	arg1	Monochlorotriazinyl-β-cyclodextrin					159:192	Monochlorotriazinyl-β-cyclodextrin	159:192	Monochlorotriazinyl-β-cyclodextrin	159:192	Preparation and Characterization of Semi-IPN Cryogels Based on Polyacrylamide and Poly(N,N-dimethylaminoethyl methacrylate); Functionalization of Carrier with Monochlorotriazinyl-β-cyclodextrin and Release Kinetics of Curcumin.
34834067	0	56	with	Functionalization	125:141	arg1	Kinetics					206:213	Release Kinetics	198:213	Release Kinetics	198:213	Preparation and Characterization of Semi-IPN Cryogels Based on Polyacrylamide and Poly(N,N-dimethylaminoethyl methacrylate); Functionalization of Carrier with Monochlorotriazinyl-β-cyclodextrin and Release Kinetics of Curcumin.
34834067	3	57	theme	networks	791:798	arg1	preparation					746:756	the preparation	742:756	the preparation of semi-interpenetrating polymer networks	742:798	To enhance the bioavailability of CCM, and to protect it against the external degradation agents, a novel strategy, which consists in the preparation of semi-interpenetrating polymer networks, (s-IPNs) based on poly(N,N-dimethylaminoethyl methacrylate) entrapped in poly(acrylamide) networks, by a cryogelation technique, was developed in this work.
34834067	1	58	theme	anti-inflammatory	417:433	arg1	properties					496:505	its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties	413:505	its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties	413:505	Curcumin (CCM) is a natural hydrophobic polyphenol known for its numerous applications in the food industry as a colorant or jelly stabilizer, and in the pharmaceutical industry due to its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties.
34834067	7	59	theme	kinetic	1510:1516	arg1	models					1518:1523	Higuchi, Korsmeyer-Peppas, and first order kinetic models	1467:1523	Higuchi, Korsmeyer-Peppas, and first order kinetic models on the CCM release profiles	1467:1551	Fitting Higuchi, Korsmeyer-Peppas, and first order kinetic models on the CCM release profiles indicated the diffusion as the main driving force of drug release from the composite cryogels.
34834067	7	60	theme	release	1611:1617	arg1	force					1597:1601	the main driving force	1580:1601	the main driving force of drug release from the composite cryogels	1580:1645	Fitting Higuchi, Korsmeyer-Peppas, and first order kinetic models on the CCM release profiles indicated the diffusion as the main driving force of drug release from the composite cryogels.
34834067	7	61	theme	Higuchi	1467:1473	arg1	models					1518:1523	Higuchi, Korsmeyer-Peppas, and first order kinetic models	1467:1523	Higuchi, Korsmeyer-Peppas, and first order kinetic models on the CCM release profiles	1467:1551	Fitting Higuchi, Korsmeyer-Peppas, and first order kinetic models on the CCM release profiles indicated the diffusion as the main driving force of drug release from the composite cryogels.
34834067	4	62	theme	Functionalization	1064:1080	arg1	function					1048:1055	a function	1046:1055	a function of pH. Functionalization of semi-IPN cryogel with monochlorotriazinyl-β-cyclodextrin (MCT-β-CD) led to IPN cryogel	1046:1170	All s-IPN cryogels were characterized by SEM, EDX, FTIR, and swelling at equilibrium as a function of pH. Functionalization of semi-IPN cryogel with monochlorotriazinyl-β-cyclodextrin (MCT-β-CD) led to IPN cryogel.
34834067	4	62	theme	Functionalization	1064:1080	arg1	cryogels					968:975	All s-IPN cryogels	958:975	All s-IPN cryogels	958:975	All s-IPN cryogels were characterized by SEM, EDX, FTIR, and swelling at equilibrium as a function of pH. Functionalization of semi-IPN cryogel with monochlorotriazinyl-β-cyclodextrin (MCT-β-CD) led to IPN cryogel.
34834067	5	63	theme	composite	1209:1217	arg1	cryogels					1219:1226	the composite cryogels	1205:1226	the composite cryogels	1205:1226	The release profile of CCM from the composite cryogels was investigated at 37 °C, in pH 3.
34834067	3	64	theme	semi-interpenetrating	761:781	arg1	networks					791:798	semi-interpenetrating polymer networks	761:798	semi-interpenetrating polymer networks	761:798	To enhance the bioavailability of CCM, and to protect it against the external degradation agents, a novel strategy, which consists in the preparation of semi-interpenetrating polymer networks, (s-IPNs) based on poly(N,N-dimethylaminoethyl methacrylate) entrapped in poly(acrylamide) networks, by a cryogelation technique, was developed in this work.
34834067	6	65	theme	cumulative	1286:1295	arg1	release					1297:1303	the cumulative release	1282:1303	the cumulative release	1282:1303	It was found that the cumulative release increased with the increase of the carrier hydrophobicity, as a result of increasing the cross-linking degree, the content and the molar mass of PDMAEMA.
34834067	3	66	theme	external	677:684	arg1	s-IPNs					802:807	s-IPNs	802:807	s-IPNs	802:807	To enhance the bioavailability of CCM, and to protect it against the external degradation agents, a novel strategy, which consists in the preparation of semi-interpenetrating polymer networks, (s-IPNs) based on poly(N,N-dimethylaminoethyl methacrylate) entrapped in poly(acrylamide) networks, by a cryogelation technique, was developed in this work.
34834067	3	66	theme	external	677:684	arg1	strategy					714:721	a novel strategy	706:721	a novel strategy	706:721	To enhance the bioavailability of CCM, and to protect it against the external degradation agents, a novel strategy, which consists in the preparation of semi-interpenetrating polymer networks, (s-IPNs) based on poly(N,N-dimethylaminoethyl methacrylate) entrapped in poly(acrylamide) networks, by a cryogelation technique, was developed in this work.
34834067	3	66	theme	external	677:684	arg1	agents					698:703	the external degradation agents	673:703	the external degradation agents	673:703	To enhance the bioavailability of CCM, and to protect it against the external degradation agents, a novel strategy, which consists in the preparation of semi-interpenetrating polymer networks, (s-IPNs) based on poly(N,N-dimethylaminoethyl methacrylate) entrapped in poly(acrylamide) networks, by a cryogelation technique, was developed in this work.
34834067	3	67	theme	polymer	783:789	arg1	networks					791:798	semi-interpenetrating polymer networks	761:798	semi-interpenetrating polymer networks	761:798	To enhance the bioavailability of CCM, and to protect it against the external degradation agents, a novel strategy, which consists in the preparation of semi-interpenetrating polymer networks, (s-IPNs) based on poly(N,N-dimethylaminoethyl methacrylate) entrapped in poly(acrylamide) networks, by a cryogelation technique, was developed in this work.
34834067	4	68	theme	cryogel	1094:1100	arg1	Functionalization					1064:1080	pH. Functionalization	1060:1080	pH. Functionalization of semi-IPN cryogel with monochlorotriazinyl-β-cyclodextrin (MCT-β-CD) led to IPN cryogel	1060:1170	All s-IPN cryogels were characterized by SEM, EDX, FTIR, and swelling at equilibrium as a function of pH. Functionalization of semi-IPN cryogel with monochlorotriazinyl-β-cyclodextrin (MCT-β-CD) led to IPN cryogel.
34834067	7	69	theme	Korsmeyer-Peppas	1476:1491	arg1	models					1518:1523	Higuchi, Korsmeyer-Peppas, and first order kinetic models	1467:1523	Higuchi, Korsmeyer-Peppas, and first order kinetic models on the CCM release profiles	1467:1551	Fitting Higuchi, Korsmeyer-Peppas, and first order kinetic models on the CCM release profiles indicated the diffusion as the main driving force of drug release from the composite cryogels.
34834067	1	70	theme	natural	248:254	arg1	polyphenol					268:277	a natural hydrophobic polyphenol	246:277	a natural hydrophobic polyphenol known for its numerous applications in the food industry as a colorant or jelly stabilizer, and in the pharmaceutical industry due to its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties	246:505	Curcumin (CCM) is a natural hydrophobic polyphenol known for its numerous applications in the food industry as a colorant or jelly stabilizer, and in the pharmaceutical industry due to its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties.
34834067	1	70	theme	natural	248:254	arg1	Curcumin					228:235	Curcumin	228:235	Curcumin (CCM)	228:241	Curcumin (CCM) is a natural hydrophobic polyphenol known for its numerous applications in the food industry as a colorant or jelly stabilizer, and in the pharmaceutical industry due to its anti-inflammatory, antibacterial, antioxidant, anti-cancer, and anti-Alzheimer properties.
32841408	8	0	theme	peak	946:949	arg1	percentage					956:965	their peak area percentage	940:965	their peak area percentage	940:965	Compared with CZE method in European Pharmacopoeia, the numbers of isoforms and their peak area percentage were highly consistent.
32841408	7	1	theme	ammonium	732:739	arg1	solution					751:758	100 mM ammonium hydroxide solution	725:758	100 mM ammonium hydroxide solution	725:758	A weak base (100 mM ammonium hydroxide solution) was used as a chemical mobilizer to make the acidic bands mobilize stably to the detection window.
32841408	7	1	theme	ammonium	732:739	arg1	base					719:722	A weak base	712:722	A weak base (100 mM ammonium hydroxide solution)	712:759	A weak base (100 mM ammonium hydroxide solution) was used as a chemical mobilizer to make the acidic bands mobilize stably to the detection window.
32841408	11	2	theme	Harmonization	1343:1355	arg1	guidelines					1363:1372	Harmonization (ICH) guidelines	1343:1372	Harmonization (ICH) guidelines	1343:1372	It was also the first time that the cIEF method was fully validated for rhuEPO analysis according to the International Conference on Harmonization (ICH) guidelines.
32841408	7	3	used	used	765:768	arg2	base					719:722	A weak base	712:722	A weak base (100 mM ammonium hydroxide solution)	712:759	A weak base (100 mM ammonium hydroxide solution) was used as a chemical mobilizer to make the acidic bands mobilize stably to the detection window.
32841408	7	3	used	used	765:768	arg2	mobilizer					784:792	a chemical mobilizer	773:792	a chemical mobilizer to make the acidic bands mobilize stably to the detection window	773:857	A weak base (100 mM ammonium hydroxide solution) was used as a chemical mobilizer to make the acidic bands mobilize stably to the detection window.
32841408	7	3	used	used	765:768	arg2	solution					751:758	100 mM ammonium hydroxide solution	725:758	100 mM ammonium hydroxide solution	725:758	A weak base (100 mM ammonium hydroxide solution) was used as a chemical mobilizer to make the acidic bands mobilize stably to the detection window.
32841408	11	4	theme	cIEF	1246:1249	arg1	method					1251:1256	the cIEF method	1242:1256	the cIEF method	1242:1256	It was also the first time that the cIEF method was fully validated for rhuEPO analysis according to the International Conference on Harmonization (ICH) guidelines.
32841408	7	5	theme	hydroxide	741:749	arg1	solution					751:758	100 mM ammonium hydroxide solution	725:758	100 mM ammonium hydroxide solution	725:758	A weak base (100 mM ammonium hydroxide solution) was used as a chemical mobilizer to make the acidic bands mobilize stably to the detection window.
32841408	7	5	theme	hydroxide	741:749	arg1	base					719:722	A weak base	712:722	A weak base (100 mM ammonium hydroxide solution)	712:759	A weak base (100 mM ammonium hydroxide solution) was used as a chemical mobilizer to make the acidic bands mobilize stably to the detection window.
32841408	10	6	used	used	1185:1188	arg2	pI					1143:1144	pI	1143:1144	pI	1143:1144	Moreover, in improved cIEF method, the isoelectric points (pI) of each isoform can be calculated and used for identification.
32841408	10	6	used	used	1185:1188	arg2	points					1135:1140	the isoelectric points	1119:1140	the isoelectric points (pI) of each isoform	1119:1161	Moreover, in improved cIEF method, the isoelectric points (pI) of each isoform can be calculated and used for identification.
32841408	7	7	theme	100 mM	725:730	arg1	solution					751:758	100 mM ammonium hydroxide solution	725:758	100 mM ammonium hydroxide solution	725:758	A weak base (100 mM ammonium hydroxide solution) was used as a chemical mobilizer to make the acidic bands mobilize stably to the detection window.
32841408	7	7	theme	100 mM	725:730	arg1	base					719:722	A weak base	712:722	A weak base (100 mM ammonium hydroxide solution)	712:759	A weak base (100 mM ammonium hydroxide solution) was used as a chemical mobilizer to make the acidic bands mobilize stably to the detection window.
32841408	3	8	with	complex	415:421	arg1	isoforms					436:443	several isoforms	428:443	several isoforms	428:443	Because of the heterogeneity of glycan, the structure of rhuEPO was complex with several isoforms.
32841408	9	9	theme	improved	1062:1069	arg1	method					1076:1081	the improved cIEF method	1058:1081	the improved cIEF method	1058:1081	Better reproducibility and higher resolution have been obtained by the improved cIEF method.
32841408	8	10	from	method	878:883	arg1	Pharmacopoeia					897:909	European Pharmacopoeia	888:909	European Pharmacopoeia	888:909	Compared with CZE method in European Pharmacopoeia, the numbers of isoforms and their peak area percentage were highly consistent.
32841408	7	11	dep	bands	813:817	arg1	mobilize					819:826	mobilize	819:826	mobilize stably to the detection window	819:857	A weak base (100 mM ammonium hydroxide solution) was used as a chemical mobilizer to make the acidic bands mobilize stably to the detection window.
32841408	10	12	theme	cIEF	1106:1109	arg1	method					1111:1116	improved cIEF method	1097:1116	improved cIEF method	1097:1116	Moreover, in improved cIEF method, the isoelectric points (pI) of each isoform can be calculated and used for identification.
32841408	11	13	from	Conference	1329:1338	arg1	guidelines					1363:1372	Harmonization (ICH) guidelines	1343:1372	Harmonization (ICH) guidelines	1343:1372	It was also the first time that the cIEF method was fully validated for rhuEPO analysis according to the International Conference on Harmonization (ICH) guidelines.
32841408	9	14	theme	cIEF	1071:1074	arg1	method					1076:1081	the improved cIEF method	1058:1081	the improved cIEF method	1058:1081	Better reproducibility and higher resolution have been obtained by the improved cIEF method.
32841408	7	15	theme	detection	842:850	arg1	window					852:857	the detection window	838:857	the detection window	838:857	A weak base (100 mM ammonium hydroxide solution) was used as a chemical mobilizer to make the acidic bands mobilize stably to the detection window.
32841408	10	16	theme	improved	1097:1104	arg1	method					1111:1116	improved cIEF method	1097:1116	improved cIEF method	1097:1116	Moreover, in improved cIEF method, the isoelectric points (pI) of each isoform can be calculated and used for identification.
32841408	1	17	used	used	170:173	arg2	rhuEPO					141:146	rhuEPO	141:146	rhuEPO	141:146	Recombinant human erythropoietin (rhuEPO) has been extensively used as a pharmaceutical product for treating anemia in the clinic.
32841408	1	17	used	used	170:173	arg2	erythropoietin					125:138	Recombinant human erythropoietin	107:138	Recombinant human erythropoietin (rhuEPO)	107:147	Recombinant human erythropoietin (rhuEPO) has been extensively used as a pharmaceutical product for treating anemia in the clinic.
32841408	1	17	used	used	170:173	arg2	product					195:201	a pharmaceutical product	178:201	a pharmaceutical product for treating anemia in the clinic	178:235	Recombinant human erythropoietin (rhuEPO) has been extensively used as a pharmaceutical product for treating anemia in the clinic.
32841408	1	18	theme	pharmaceutical	180:193	arg1	erythropoietin					125:138	Recombinant human erythropoietin	107:138	Recombinant human erythropoietin (rhuEPO)	107:147	Recombinant human erythropoietin (rhuEPO) has been extensively used as a pharmaceutical product for treating anemia in the clinic.
32841408	1	18	theme	pharmaceutical	180:193	arg1	product					195:201	a pharmaceutical product	178:201	a pharmaceutical product for treating anemia in the clinic	178:235	Recombinant human erythropoietin (rhuEPO) has been extensively used as a pharmaceutical product for treating anemia in the clinic.
32841408	3	19	theme	glycan	379:384	arg1	heterogeneity					362:374	the heterogeneity	358:374	the heterogeneity of glycan	358:384	Because of the heterogeneity of glycan, the structure of rhuEPO was complex with several isoforms.
32841408	0	20	theme	erythropoietin	39:52	arg1	analysis					9:16	analysis	9:16	analysis of recombinant human erythropoietin	9:52	Isoforms analysis of recombinant human erythropoietin by polarity-reversed capillary isoelectric focusing.
32841408	2	21	theme	biological	288:297	arg1	activity					299:306	biological activity	288:306	biological activity	288:306	Glycosylation of rhuEPO was crucial for affecting biological activity, immunogenicity, and pharmacokinetics.
32841408	3	22	theme	rhuEPO	404:409	arg1	structure					391:399	the structure	387:399	the structure of rhuEPO	387:409	Because of the heterogeneity of glycan, the structure of rhuEPO was complex with several isoforms.
32841408	3	22	theme	rhuEPO	404:409	arg1	complex					415:421	complex	415:421	complex	415:421	Because of the heterogeneity of glycan, the structure of rhuEPO was complex with several isoforms.
32841408	4	23	theme	rhuEPO	512:517	arg1	control					501:507	quality control	493:507	quality control of rhuEPO	493:517	Characterization of isoforms was important for quality control of rhuEPO.
32841408	1	24	theme	Recombinant	107:117	arg1	rhuEPO					141:146	rhuEPO	141:146	rhuEPO	141:146	Recombinant human erythropoietin (rhuEPO) has been extensively used as a pharmaceutical product for treating anemia in the clinic.
32841408	1	24	theme	Recombinant	107:117	arg1	erythropoietin					125:138	Recombinant human erythropoietin	107:138	Recombinant human erythropoietin (rhuEPO)	107:147	Recombinant human erythropoietin (rhuEPO) has been extensively used as a pharmaceutical product for treating anemia in the clinic.
32841408	1	24	theme	Recombinant	107:117	arg1	product					195:201	a pharmaceutical product	178:201	a pharmaceutical product for treating anemia in the clinic	178:235	Recombinant human erythropoietin (rhuEPO) has been extensively used as a pharmaceutical product for treating anemia in the clinic.
32841408	5	25	theme	improved	529:536	arg1	method					543:548	an improved cIEF method	526:548	an improved cIEF method	526:548	Here, an improved cIEF method has been established and validated.
32841408	10	26	theme	isoelectric	1123:1133	arg1	pI					1143:1144	pI	1143:1144	pI	1143:1144	Moreover, in improved cIEF method, the isoelectric points (pI) of each isoform can be calculated and used for identification.
32841408	10	26	theme	isoelectric	1123:1133	arg1	points					1135:1140	the isoelectric points	1119:1140	the isoelectric points (pI) of each isoform	1119:1161	Moreover, in improved cIEF method, the isoelectric points (pI) of each isoform can be calculated and used for identification.
32841408	1	27	theme	human	119:123	arg1	rhuEPO					141:146	rhuEPO	141:146	rhuEPO	141:146	Recombinant human erythropoietin (rhuEPO) has been extensively used as a pharmaceutical product for treating anemia in the clinic.
32841408	1	27	theme	human	119:123	arg1	erythropoietin					125:138	Recombinant human erythropoietin	107:138	Recombinant human erythropoietin (rhuEPO)	107:147	Recombinant human erythropoietin (rhuEPO) has been extensively used as a pharmaceutical product for treating anemia in the clinic.
32841408	1	27	theme	human	119:123	arg1	product					195:201	a pharmaceutical product	178:201	a pharmaceutical product for treating anemia in the clinic	178:235	Recombinant human erythropoietin (rhuEPO) has been extensively used as a pharmaceutical product for treating anemia in the clinic.
32841408	8	28	theme	European	888:895	arg1	Pharmacopoeia					897:909	European Pharmacopoeia	888:909	European Pharmacopoeia	888:909	Compared with CZE method in European Pharmacopoeia, the numbers of isoforms and their peak area percentage were highly consistent.
32841408	0	29	theme	recombinant	21:31	arg1	erythropoietin					39:52	recombinant human erythropoietin	21:52	recombinant human erythropoietin	21:52	Isoforms analysis of recombinant human erythropoietin by polarity-reversed capillary isoelectric focusing.
32841408	7	30	theme	acidic	806:811	arg1	bands					813:817	the acidic bands	802:817	the acidic bands mobilize stably to the detection window	802:857	A weak base (100 mM ammonium hydroxide solution) was used as a chemical mobilizer to make the acidic bands mobilize stably to the detection window.
32841408	6	31	theme	anolyte	697:703	arg1	vials					705:709	the catholyte and anolyte vials	679:709	the catholyte and anolyte vials	679:709	A polarity-reversed focusing step was used by reversing both the polarity of the voltage and the catholyte and anolyte vials.
32841408	11	32	theme	rhuEPO	1282:1287	arg1	analysis					1289:1296	rhuEPO analysis	1282:1296	rhuEPO analysis according to the International Conference on Harmonization (ICH) guidelines	1282:1372	It was also the first time that the cIEF method was fully validated for rhuEPO analysis according to the International Conference on Harmonization (ICH) guidelines.
32841408	7	33	theme	weak	714:717	arg1	mobilizer					784:792	a chemical mobilizer	773:792	a chemical mobilizer to make the acidic bands mobilize stably to the detection window	773:857	A weak base (100 mM ammonium hydroxide solution) was used as a chemical mobilizer to make the acidic bands mobilize stably to the detection window.
32841408	7	33	theme	weak	714:717	arg1	solution					751:758	100 mM ammonium hydroxide solution	725:758	100 mM ammonium hydroxide solution	725:758	A weak base (100 mM ammonium hydroxide solution) was used as a chemical mobilizer to make the acidic bands mobilize stably to the detection window.
32841408	7	33	theme	weak	714:717	arg1	base					719:722	A weak base	712:722	A weak base (100 mM ammonium hydroxide solution)	712:759	A weak base (100 mM ammonium hydroxide solution) was used as a chemical mobilizer to make the acidic bands mobilize stably to the detection window.
32841408	8	34	theme	CZE	874:876	arg1	method					878:883	CZE method	874:883	CZE method in European Pharmacopoeia	874:909	Compared with CZE method in European Pharmacopoeia, the numbers of isoforms and their peak area percentage were highly consistent.
32841408	3	35	theme	several	428:434	arg1	isoforms					436:443	several isoforms	428:443	several isoforms	428:443	Because of the heterogeneity of glycan, the structure of rhuEPO was complex with several isoforms.
32841408	2	36	theme	rhuEPO	255:260	arg1	Glycosylation					238:250	Glycosylation	238:250	Glycosylation of rhuEPO	238:260	Glycosylation of rhuEPO was crucial for affecting biological activity, immunogenicity, and pharmacokinetics.
32841408	6	37	theme	catholyte	683:691	arg1	vials					705:709	the catholyte and anolyte vials	679:709	the catholyte and anolyte vials	679:709	A polarity-reversed focusing step was used by reversing both the polarity of the voltage and the catholyte and anolyte vials.
32841408	0	38	theme	human	33:37	arg1	erythropoietin					39:52	recombinant human erythropoietin	21:52	recombinant human erythropoietin	21:52	Isoforms analysis of recombinant human erythropoietin by polarity-reversed capillary isoelectric focusing.
32841408	7	39	theme	chemical	775:782	arg1	mobilizer					784:792	a chemical mobilizer	773:792	a chemical mobilizer to make the acidic bands mobilize stably to the detection window	773:857	A weak base (100 mM ammonium hydroxide solution) was used as a chemical mobilizer to make the acidic bands mobilize stably to the detection window.
32841408	7	39	theme	chemical	775:782	arg1	base					719:722	A weak base	712:722	A weak base (100 mM ammonium hydroxide solution)	712:759	A weak base (100 mM ammonium hydroxide solution) was used as a chemical mobilizer to make the acidic bands mobilize stably to the detection window.
32841408	6	40	theme	focusing	606:613	arg1	step					615:618	A polarity-reversed focusing step	586:618	A polarity-reversed focusing step	586:618	A polarity-reversed focusing step was used by reversing both the polarity of the voltage and the catholyte and anolyte vials.
32841408	4	41	theme	quality	493:499	arg1	control					501:507	quality control	493:507	quality control of rhuEPO	493:517	Characterization of isoforms was important for quality control of rhuEPO.
32841408	6	42	theme	polarity-reversed	588:604	arg1	step					615:618	A polarity-reversed focusing step	586:618	A polarity-reversed focusing step	586:618	A polarity-reversed focusing step was used by reversing both the polarity of the voltage and the catholyte and anolyte vials.
32841408	3	43	gly	heterogeneity	362:374	arg1	glycan					379:384	glycan	379:384	glycan	379:384	Because of the heterogeneity of glycan, the structure of rhuEPO was complex with several isoforms.
32841408	11	44	theme	ICH	1358:1360	arg1	guidelines					1363:1372	Harmonization (ICH) guidelines	1343:1372	Harmonization (ICH) guidelines	1343:1372	It was also the first time that the cIEF method was fully validated for rhuEPO analysis according to the International Conference on Harmonization (ICH) guidelines.
32841408	2	45	gly	Glycosylation	238:250	arg1	rhuEPO					255:260	rhuEPO	255:260	rhuEPO	255:260	Glycosylation of rhuEPO was crucial for affecting biological activity, immunogenicity, and pharmacokinetics.
32841408	11	46	theme	first	1226:1230	arg1	time					1232:1235	the first time that the cIEF method was fully validated for rhuEPO analysis according to the International Conference on Harmonization (ICH) guidelines	1222:1372	the first time that the cIEF method was fully validated for rhuEPO analysis according to the International Conference on Harmonization (ICH) guidelines	1222:1372	It was also the first time that the cIEF method was fully validated for rhuEPO analysis according to the International Conference on Harmonization (ICH) guidelines.
32841408	11	46	theme	first	1226:1230	arg1	It					1210:1211	It	1210:1211	It	1210:1211	It was also the first time that the cIEF method was fully validated for rhuEPO analysis according to the International Conference on Harmonization (ICH) guidelines.
32841408	9	47	theme	Better	991:996	arg1	reproducibility					998:1012	Better reproducibility	991:1012	Better reproducibility	991:1012	Better reproducibility and higher resolution have been obtained by the improved cIEF method.
32841408	8	48	theme	isoforms	927:934	arg1	percentage					956:965	their peak area percentage	940:965	their peak area percentage	940:965	Compared with CZE method in European Pharmacopoeia, the numbers of isoforms and their peak area percentage were highly consistent.
32841408	8	48	theme	isoforms	927:934	arg1	consistent					979:988	consistent	979:988	consistent	979:988	Compared with CZE method in European Pharmacopoeia, the numbers of isoforms and their peak area percentage were highly consistent.
32841408	8	48	theme	isoforms	927:934	arg1	numbers					916:922	the numbers	912:922	the numbers of isoforms	912:934	Compared with CZE method in European Pharmacopoeia, the numbers of isoforms and their peak area percentage were highly consistent.
32841408	9	49	theme	higher	1018:1023	arg1	resolution					1025:1034	higher resolution	1018:1034	higher resolution	1018:1034	Better reproducibility and higher resolution have been obtained by the improved cIEF method.
32841408	6	50	used	used	624:627	arg2	step					615:618	A polarity-reversed focusing step	586:618	A polarity-reversed focusing step	586:618	A polarity-reversed focusing step was used by reversing both the polarity of the voltage and the catholyte and anolyte vials.
32841408	11	51	theme	International	1315:1327	arg1	Conference					1329:1338	the International Conference	1311:1338	the International Conference on Harmonization (ICH) guidelines	1311:1372	It was also the first time that the cIEF method was fully validated for rhuEPO analysis according to the International Conference on Harmonization (ICH) guidelines.
32841408	4	52	theme	isoforms	466:473	arg1	Characterization					446:461	Characterization	446:461	Characterization of isoforms	446:473	Characterization of isoforms was important for quality control of rhuEPO.
32841408	5	53	theme	cIEF	538:541	arg1	method					543:548	an improved cIEF method	526:548	an improved cIEF method	526:548	Here, an improved cIEF method has been established and validated.
32841408	6	54	theme	voltage	667:673	arg1	polarity					651:658	the polarity	647:658	the polarity of the voltage	647:673	A polarity-reversed focusing step was used by reversing both the polarity of the voltage and the catholyte and anolyte vials.
32841408	6	54	theme	voltage	667:673	arg1	vials					705:709	the catholyte and anolyte vials	679:709	the catholyte and anolyte vials	679:709	A polarity-reversed focusing step was used by reversing both the polarity of the voltage and the catholyte and anolyte vials.
32841408	10	55	theme	isoform	1155:1161	arg1	pI					1143:1144	pI	1143:1144	pI	1143:1144	Moreover, in improved cIEF method, the isoelectric points (pI) of each isoform can be calculated and used for identification.
32841408	10	55	theme	isoform	1155:1161	arg1	points					1135:1140	the isoelectric points	1119:1140	the isoelectric points (pI) of each isoform	1119:1161	Moreover, in improved cIEF method, the isoelectric points (pI) of each isoform can be calculated and used for identification.
32841408	3	56	theme	heterogeneity	362:374	arg1	structure					391:399	the structure	387:399	the structure of rhuEPO	387:409	Because of the heterogeneity of glycan, the structure of rhuEPO was complex with several isoforms.
32841408	3	56	theme	heterogeneity	362:374	arg1	complex					415:421	complex	415:421	complex	415:421	Because of the heterogeneity of glycan, the structure of rhuEPO was complex with several isoforms.
32841408	8	57	theme	area	951:954	arg1	percentage					956:965	their peak area percentage	940:965	their peak area percentage	940:965	Compared with CZE method in European Pharmacopoeia, the numbers of isoforms and their peak area percentage were highly consistent.
33140127	6	0	from	70 eV	1316:1320	arg1	benefits					1302:1309	benefits	1302:1309	benefits from 70 eV and 12 eV resulting in more comprehensive coverage by sample fingerprints	1302:1394	Fused data combine benefits from 70 eV and 12 eV resulting in more comprehensive coverage by sample fingerprints.
33140127	9	1	from	amount	1918:1923	arg1	saliva					1928:1933	saliva	1928:1933	saliva from MHO patients	1928:1951	Within the most informative analytes, lactose is present in higher relative amount in saliva from MHO patients, whereas N-acetyl-D-glucosamine, urea, glucuronic acid γ-lactone, 2-deoxyribose, N-acetylneuraminic acid methyl ester, and 5-aminovaleric acid are more abundant in MUO patients.
33140127	9	1	from	amount	1918:1923	arg1	patients					1944:1951	MHO patients	1940:1951	MHO patients	1940:1951	Within the most informative analytes, lactose is present in higher relative amount in saliva from MHO patients, whereas N-acetyl-D-glucosamine, urea, glucuronic acid γ-lactone, 2-deoxyribose, N-acetylneuraminic acid methyl ester, and 5-aminovaleric acid are more abundant in MUO patients.
33140127	9	1	from	amount	1918:1923	arg1	present					1891:1897	present	1891:1897	present	1891:1897	Within the most informative analytes, lactose is present in higher relative amount in saliva from MHO patients, whereas N-acetyl-D-glucosamine, urea, glucuronic acid γ-lactone, 2-deoxyribose, N-acetylneuraminic acid methyl ester, and 5-aminovaleric acid are more abundant in MUO patients.
33140127	0	2	theme	Tandem	187:192	arg1	spectrometry					210:221	Tandem Ionization mass spectrometry	187:221	Tandem Ionization mass spectrometry	187:221	Exploring extra dimensions to capture saliva metabolite fingerprints from metabolically healthy and unhealthy obese patients by comprehensive two-dimensional gas chromatography featuring Tandem Ionization mass spectrometry.
33140127	9	3	theme	N-acetylneuraminic	2034:2051	arg1	ester					2065:2069	N-acetylneuraminic acid methyl ester	2034:2069	N-acetylneuraminic acid methyl ester	2034:2069	Within the most informative analytes, lactose is present in higher relative amount in saliva from MHO patients, whereas N-acetyl-D-glucosamine, urea, glucuronic acid γ-lactone, 2-deoxyribose, N-acetylneuraminic acid methyl ester, and 5-aminovaleric acid are more abundant in MUO patients.
33140127	9	4	from	patients	2121:2128	arg1	abundant					2105:2112	abundant	2105:2112	abundant	2105:2112	Within the most informative analytes, lactose is present in higher relative amount in saliva from MHO patients, whereas N-acetyl-D-glucosamine, urea, glucuronic acid γ-lactone, 2-deoxyribose, N-acetylneuraminic acid methyl ester, and 5-aminovaleric acid are more abundant in MUO patients.
33140127	2	5	theme	data	648:651	arg1	nature					620:625	the complementary nature	602:625	the complementary nature of tandem ionization data	602:651	The study presents a proof of concept for an effective exploitation of the complementary nature of tandem ionization data.
33140127	7	6	theme	Multivariate	1397:1408	arg1	statistics					1410:1419	Multivariate statistics	1397:1419	Multivariate statistics	1397:1419	Multivariate statistics, principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA) show quite good patient clustering, with total explained variance by the first two principal components (PCs) that increases from 54% at 70 eV to 59% at 12 eV and up to 71% for fused data.
33140127	7	7	dep	71	1684:1685	arg1	to					1681:1682	to	1681:1682	to	1681:1682	Multivariate statistics, principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA) show quite good patient clustering, with total explained variance by the first two principal components (PCs) that increases from 54% at 70 eV to 59% at 12 eV and up to 71% for fused data.
33140127	9	8	theme	methyl	2058:2063	arg1	ester					2065:2069	N-acetylneuraminic acid methyl ester	2034:2069	N-acetylneuraminic acid methyl ester	2034:2069	Within the most informative analytes, lactose is present in higher relative amount in saliva from MHO patients, whereas N-acetyl-D-glucosamine, urea, glucuronic acid γ-lactone, 2-deoxyribose, N-acetylneuraminic acid methyl ester, and 5-aminovaleric acid are more abundant in MUO patients.
33140127	1	9	theme	time-of-flight	336:349	arg1	spectrometry					356:367	time-of-flight mass spectrometry	336:367	time-of-flight mass spectrometry (GC×GC-TOF MS)	336:382	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	1	9	theme	time-of-flight	336:349	arg1	MS					380:381	GC×GC-TOF MS	370:381	GC×GC-TOF MS	370:381	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	10	10	theme	composite	2252:2260	arg1	images					2272:2277	composite per-class images	2252:2277	composite per-class images obtained by combining raw data from individuals belonging to different classes, i.e., MUO vs. MHO.Graphical abstract	2252:2394	Visual feature fingerprinting is combined with pattern recognition algorithms to highlight metabolite variations between composite per-class images obtained by combining raw data from individuals belonging to different classes, i.e., MUO vs. MHO.Graphical abstract.
33140127	10	11	theme	raw	2301:2303	arg1	data					2305:2308	raw data	2301:2308	raw data from individuals belonging to different classes, i.e., MUO vs. MHO.Graphical abstract	2301:2394	Visual feature fingerprinting is combined with pattern recognition algorithms to highlight metabolite variations between composite per-class images obtained by combining raw data from individuals belonging to different classes, i.e., MUO vs. MHO.Graphical abstract.
33140127	1	12	theme	obese	512:516	arg1	individuals					518:528	obese individuals	512:528	obese individuals	512:528	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	7	13	theme	principal	1422:1430	arg1	PCA					1452:1454	PCA	1452:1454	PCA	1452:1454	Multivariate statistics, principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA) show quite good patient clustering, with total explained variance by the first two principal components (PCs) that increases from 54% at 70 eV to 59% at 12 eV and up to 71% for fused data.
33140127	7	13	theme	principal	1422:1430	arg1	analysis					1442:1449	principal component analysis	1422:1449	principal component analysis (PCA)	1422:1455	Multivariate statistics, principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA) show quite good patient clustering, with total explained variance by the first two principal components (PCs) that increases from 54% at 70 eV to 59% at 12 eV and up to 71% for fused data.
33140127	9	14	theme	5-aminovaleric	2076:2089	arg1	acid					2091:2094	5-aminovaleric acid	2076:2094	5-aminovaleric acid	2076:2094	Within the most informative analytes, lactose is present in higher relative amount in saliva from MHO patients, whereas N-acetyl-D-glucosamine, urea, glucuronic acid γ-lactone, 2-deoxyribose, N-acetylneuraminic acid methyl ester, and 5-aminovaleric acid are more abundant in MUO patients.
33140127	7	15	theme	principal	1598:1606	arg1	PCs					1620:1622	PCs	1620:1622	PCs	1620:1622	Multivariate statistics, principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA) show quite good patient clustering, with total explained variance by the first two principal components (PCs) that increases from 54% at 70 eV to 59% at 12 eV and up to 71% for fused data.
33140127	7	15	theme	principal	1598:1606	arg1	components					1608:1617	the first two principal components	1584:1617	the first two principal components (PCs) that increases from 54% at 70 eV to 59% at 12 eV and up to 71% for fused data	1584:1701	Multivariate statistics, principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA) show quite good patient clustering, with total explained variance by the first two principal components (PCs) that increases from 54% at 70 eV to 59% at 12 eV and up to 71% for fused data.
33140127	0	16	theme	comprehensive	128:140	arg1	chromatography					162:175	comprehensive two-dimensional gas chromatography	128:175	comprehensive two-dimensional gas chromatography featuring Tandem Ionization mass spectrometry	128:221	Exploring extra dimensions to capture saliva metabolite fingerprints from metabolically healthy and unhealthy obese patients by comprehensive two-dimensional gas chromatography featuring Tandem Ionization mass spectrometry.
33140127	3	17	dep	taken	666:670	arg1	named					742:746	named	742:746	named metabolically healthy obese (MHO) and metabolically unhealthy obese (MUO)	742:820	Samples are taken from two sub-populations of severely obese (BMI > 40 kg/m2) patients, named metabolically healthy obese (MHO) and metabolically unhealthy obese (MUO).
33140127	7	18	theme	first	1588:1592	arg1	PCs					1620:1622	PCs	1620:1622	PCs	1620:1622	Multivariate statistics, principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA) show quite good patient clustering, with total explained variance by the first two principal components (PCs) that increases from 54% at 70 eV to 59% at 12 eV and up to 71% for fused data.
33140127	7	18	theme	first	1588:1592	arg1	components					1608:1617	the first two principal components	1584:1617	the first two principal components (PCs) that increases from 54% at 70 eV to 59% at 12 eV and up to 71% for fused data	1584:1701	Multivariate statistics, principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA) show quite good patient clustering, with total explained variance by the first two principal components (PCs) that increases from 54% at 70 eV to 59% at 12 eV and up to 71% for fused data.
33140127	10	19	from	individuals	2315:2325	arg1	data					2305:2308	raw data	2301:2308	raw data from individuals belonging to different classes, i.e., MUO vs. MHO.Graphical abstract	2301:2394	Visual feature fingerprinting is combined with pattern recognition algorithms to highlight metabolite variations between composite per-class images obtained by combining raw data from individuals belonging to different classes, i.e., MUO vs. MHO.Graphical abstract.
33140127	9	20	theme	informative	1858:1868	arg1	analytes					1870:1877	the most informative analytes	1849:1877	the most informative analytes	1849:1877	Within the most informative analytes, lactose is present in higher relative amount in saliva from MHO patients, whereas N-acetyl-D-glucosamine, urea, glucuronic acid γ-lactone, 2-deoxyribose, N-acetylneuraminic acid methyl ester, and 5-aminovaleric acid are more abundant in MUO patients.
33140127	0	21	theme	gas	158:160	arg1	chromatography					162:175	comprehensive two-dimensional gas chromatography	128:175	comprehensive two-dimensional gas chromatography featuring Tandem Ionization mass spectrometry	128:221	Exploring extra dimensions to capture saliva metabolite fingerprints from metabolically healthy and unhealthy obese patients by comprehensive two-dimensional gas chromatography featuring Tandem Ionization mass spectrometry.
33140127	2	22	theme	tandem	630:635	arg1	data					648:651	tandem ionization data	630:651	tandem ionization data	630:651	The study presents a proof of concept for an effective exploitation of the complementary nature of tandem ionization data.
33140127	4	23	theme	fused	942:946	arg1	data					948:951	fused data	942:951	fused data	942:951	Untargeted fingerprinting, based on pattern recognition by template matching, is applied on single data streams and on fused data, obtained by combining raw signals from the two ionization energies (12 and 70 eV).
33140127	9	24	theme	MUO	2117:2119	arg1	patients					2121:2128	MUO patients	2117:2128	MUO patients	2117:2128	Within the most informative analytes, lactose is present in higher relative amount in saliva from MHO patients, whereas N-acetyl-D-glucosamine, urea, glucuronic acid γ-lactone, 2-deoxyribose, N-acetylneuraminic acid methyl ester, and 5-aminovaleric acid are more abundant in MUO patients.
33140127	1	25	theme	saliva	462:467	arg1	signatures					479:488	saliva metabolic signatures	462:488	saliva metabolic signatures from a small group of obese individuals	462:528	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	4	26	theme	data	922:925	arg1	streams					927:933	single data streams	915:933	single data streams	915:933	Untargeted fingerprinting, based on pattern recognition by template matching, is applied on single data streams and on fused data, obtained by combining raw signals from the two ionization energies (12 and 70 eV).
33140127	2	27	theme	nature	620:625	arg1	exploitation					586:597	an effective exploitation	573:597	an effective exploitation of the complementary nature of tandem ionization data	573:651	The study presents a proof of concept for an effective exploitation of the complementary nature of tandem ionization data.
33140127	0	28	from	patients	116:123	arg1	fingerprints					56:67	saliva metabolite fingerprints	38:67	saliva metabolite fingerprints from metabolically healthy and unhealthy obese patients	38:123	Exploring extra dimensions to capture saliva metabolite fingerprints from metabolically healthy and unhealthy obese patients by comprehensive two-dimensional gas chromatography featuring Tandem Ionization mass spectrometry.
33140127	8	29	theme	70 eV	1815:1819	arg1	signatures					1830:1839	70 eV spectral signatures	1815:1839	70 eV spectral signatures	1815:1839	With PLS-DA, discriminant components are highlighted and putatively identified by comparing retention data and 70 eV spectral signatures.
33140127	0	30	theme	Ionization	194:203	arg1	spectrometry					210:221	Tandem Ionization mass spectrometry	187:221	Tandem Ionization mass spectrometry	187:221	Exploring extra dimensions to capture saliva metabolite fingerprints from metabolically healthy and unhealthy obese patients by comprehensive two-dimensional gas chromatography featuring Tandem Ionization mass spectrometry.
33140127	5	31	theme	lower	1062:1066	arg1	energy					1068:1073	lower energy	1062:1073	lower energy (i.e., 12 eV)	1062:1087	Results indicate that at lower energy (i.e., 12 eV), the total signal intensity is one order of magnitude lower compared to the reference signal at 70 eV, but the ranges of variations for 2D peak responses is larger, extending the dynamic range.
33140127	5	31	theme	lower	1062:1066	arg1	12 eV					1082:1086	12 eV	1082:1086	12 eV	1082:1086	Results indicate that at lower energy (i.e., 12 eV), the total signal intensity is one order of magnitude lower compared to the reference signal at 70 eV, but the ranges of variations for 2D peak responses is larger, extending the dynamic range.
33140127	10	32	theme	different	2340:2348	arg1	classes					2350:2356	different classes	2340:2356	different classes	2340:2356	Visual feature fingerprinting is combined with pattern recognition algorithms to highlight metabolite variations between composite per-class images obtained by combining raw data from individuals belonging to different classes, i.e., MUO vs. MHO.Graphical abstract.
33140127	5	33	theme	signal	1100:1105	arg1	intensity					1107:1115	the total signal intensity	1090:1115	the total signal intensity	1090:1115	Results indicate that at lower energy (i.e., 12 eV), the total signal intensity is one order of magnitude lower compared to the reference signal at 70 eV, but the ranges of variations for 2D peak responses is larger, extending the dynamic range.
33140127	5	33	theme	signal	1100:1105	arg1	lower					1143:1147	lower	1143:1147	lower	1143:1147	Results indicate that at lower energy (i.e., 12 eV), the total signal intensity is one order of magnitude lower compared to the reference signal at 70 eV, but the ranges of variations for 2D peak responses is larger, extending the dynamic range.
33140127	9	34	theme	relative	1909:1916	arg1	amount					1918:1923	higher relative amount	1902:1923	higher relative amount in saliva from MHO patients	1902:1951	Within the most informative analytes, lactose is present in higher relative amount in saliva from MHO patients, whereas N-acetyl-D-glucosamine, urea, glucuronic acid γ-lactone, 2-deoxyribose, N-acetylneuraminic acid methyl ester, and 5-aminovaleric acid are more abundant in MUO patients.
33140127	0	35	theme	healthy	88:94	arg1	patients					116:123	metabolically healthy and unhealthy obese patients	74:123	metabolically healthy and unhealthy obese patients	74:123	Exploring extra dimensions to capture saliva metabolite fingerprints from metabolically healthy and unhealthy obese patients by comprehensive two-dimensional gas chromatography featuring Tandem Ionization mass spectrometry.
33140127	2	36	theme	ionization	637:646	arg1	data					648:651	tandem ionization data	630:651	tandem ionization data	630:651	The study presents a proof of concept for an effective exploitation of the complementary nature of tandem ionization data.
33140127	7	37	theme	good	1526:1529	arg1	clustering					1539:1548	quite good patient clustering	1520:1548	quite good patient clustering	1520:1548	Multivariate statistics, principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA) show quite good patient clustering, with total explained variance by the first two principal components (PCs) that increases from 54% at 70 eV to 59% at 12 eV and up to 71% for fused data.
33140127	4	38	theme	raw	976:978	arg1	signals					980:986	raw signals	976:986	raw signals	976:986	Untargeted fingerprinting, based on pattern recognition by template matching, is applied on single data streams and on fused data, obtained by combining raw signals from the two ionization energies (12 and 70 eV).
33140127	10	39	theme	feature	2138:2144	arg1	fingerprinting					2146:2159	Visual feature fingerprinting	2131:2159	Visual feature fingerprinting	2131:2159	Visual feature fingerprinting is combined with pattern recognition algorithms to highlight metabolite variations between composite per-class images obtained by combining raw data from individuals belonging to different classes, i.e., MUO vs. MHO.Graphical abstract.
33140127	5	40	theme	peak	1228:1231	arg1	responses					1233:1241	2D peak responses	1225:1241	2D peak responses	1225:1241	Results indicate that at lower energy (i.e., 12 eV), the total signal intensity is one order of magnitude lower compared to the reference signal at 70 eV, but the ranges of variations for 2D peak responses is larger, extending the dynamic range.
33140127	2	41	theme	concept	561:567	arg1	proof					552:556	a proof	550:556	a proof of concept for an effective exploitation of the complementary nature of tandem ionization data	550:651	The study presents a proof of concept for an effective exploitation of the complementary nature of tandem ionization data.
33140127	0	42	theme	saliva	38:43	arg1	fingerprints					56:67	saliva metabolite fingerprints	38:67	saliva metabolite fingerprints from metabolically healthy and unhealthy obese patients	38:123	Exploring extra dimensions to capture saliva metabolite fingerprints from metabolically healthy and unhealthy obese patients by comprehensive two-dimensional gas chromatography featuring Tandem Ionization mass spectrometry.
33140127	1	43	theme	GC×GC-TOF	370:378	arg1	spectrometry					356:367	time-of-flight mass spectrometry	336:367	time-of-flight mass spectrometry (GC×GC-TOF MS)	336:382	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	1	43	theme	GC×GC-TOF	370:378	arg1	MS					380:381	GC×GC-TOF MS	370:381	GC×GC-TOF MS	370:381	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	1	44	theme	mass	351:354	arg1	spectrometry					356:367	time-of-flight mass spectrometry	336:367	time-of-flight mass spectrometry (GC×GC-TOF MS)	336:382	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	1	44	theme	mass	351:354	arg1	MS					380:381	GC×GC-TOF MS	370:381	GC×GC-TOF MS	370:381	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	0	45	theme	two-dimensional	142:156	arg1	chromatography					162:175	comprehensive two-dimensional gas chromatography	128:175	comprehensive two-dimensional gas chromatography featuring Tandem Ionization mass spectrometry	128:221	Exploring extra dimensions to capture saliva metabolite fingerprints from metabolically healthy and unhealthy obese patients by comprehensive two-dimensional gas chromatography featuring Tandem Ionization mass spectrometry.
33140127	3	46	theme	obese	709:713	arg1	BMI > 40 kg/m2					716:729	BMI > 40 kg/m2	716:729	BMI > 40 kg/m2	716:729	Samples are taken from two sub-populations of severely obese (BMI > 40 kg/m2) patients, named metabolically healthy obese (MHO) and metabolically unhealthy obese (MUO).
33140127	3	46	theme	obese	709:713	arg1	patients					732:739	severely obese (BMI > 40 kg/m2) patients	700:739	severely obese (BMI > 40 kg/m2) patients	700:739	Samples are taken from two sub-populations of severely obese (BMI > 40 kg/m2) patients, named metabolically healthy obese (MHO) and metabolically unhealthy obese (MUO).
33140127	4	47	theme	pattern	859:865	arg1	recognition					867:877	pattern recognition	859:877	pattern recognition by template matching	859:898	Untargeted fingerprinting, based on pattern recognition by template matching, is applied on single data streams and on fused data, obtained by combining raw signals from the two ionization energies (12 and 70 eV).
33140127	7	48	theme	discriminant	1484:1495	arg1	analysis					1497:1504	partial least squares discriminant analysis	1462:1504	partial least squares discriminant analysis (PLS-DA)	1462:1513	Multivariate statistics, principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA) show quite good patient clustering, with total explained variance by the first two principal components (PCs) that increases from 54% at 70 eV to 59% at 12 eV and up to 71% for fused data.
33140127	7	48	theme	discriminant	1484:1495	arg1	PLS-DA					1507:1512	PLS-DA	1507:1512	PLS-DA	1507:1512	Multivariate statistics, principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA) show quite good patient clustering, with total explained variance by the first two principal components (PCs) that increases from 54% at 70 eV to 59% at 12 eV and up to 71% for fused data.
33140127	6	49	theme	comprehensive	1350:1362	arg1	coverage					1364:1371	more comprehensive coverage	1345:1371	more comprehensive coverage by sample fingerprints	1345:1394	Fused data combine benefits from 70 eV and 12 eV resulting in more comprehensive coverage by sample fingerprints.
33140127	5	50	theme	magnitude	1133:1141	arg1	order					1124:1128	one order	1120:1128	one order of magnitude	1120:1141	Results indicate that at lower energy (i.e., 12 eV), the total signal intensity is one order of magnitude lower compared to the reference signal at 70 eV, but the ranges of variations for 2D peak responses is larger, extending the dynamic range.
33140127	1	51	theme	ionization	397:406	arg1	Ionization™					429:439	i.e., Tandem Ionization™	416:439	Ionization™	429:439	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	1	51	theme	ionization	397:406	arg1	energy					408:413	variable ionization energy	388:413	variable ionization energy (i.e., Tandem Ionization™)	388:440	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	7	52	theme	least	1470:1474	arg1	analysis					1497:1504	partial least squares discriminant analysis	1462:1504	partial least squares discriminant analysis (PLS-DA)	1462:1513	Multivariate statistics, principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA) show quite good patient clustering, with total explained variance by the first two principal components (PCs) that increases from 54% at 70 eV to 59% at 12 eV and up to 71% for fused data.
33140127	7	52	theme	least	1470:1474	arg1	PLS-DA					1507:1512	PLS-DA	1507:1512	PLS-DA	1507:1512	Multivariate statistics, principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA) show quite good patient clustering, with total explained variance by the first two principal components (PCs) that increases from 54% at 70 eV to 59% at 12 eV and up to 71% for fused data.
33140127	3	53	theme	patients	732:739	arg1	sub-populations					681:695	two sub-populations	677:695	two sub-populations of severely obese (BMI > 40 kg/m2) patients	677:739	Samples are taken from two sub-populations of severely obese (BMI > 40 kg/m2) patients, named metabolically healthy obese (MHO) and metabolically unhealthy obese (MUO).
33140127	9	54	from	patients	1944:1951	arg1	saliva					1928:1933	saliva	1928:1933	saliva from MHO patients	1928:1951	Within the most informative analytes, lactose is present in higher relative amount in saliva from MHO patients, whereas N-acetyl-D-glucosamine, urea, glucuronic acid γ-lactone, 2-deoxyribose, N-acetylneuraminic acid methyl ester, and 5-aminovaleric acid are more abundant in MUO patients.
33140127	9	54	from	patients	1944:1951	arg1	amount					1918:1923	higher relative amount	1902:1923	higher relative amount in saliva from MHO patients	1902:1951	Within the most informative analytes, lactose is present in higher relative amount in saliva from MHO patients, whereas N-acetyl-D-glucosamine, urea, glucuronic acid γ-lactone, 2-deoxyribose, N-acetylneuraminic acid methyl ester, and 5-aminovaleric acid are more abundant in MUO patients.
33140127	5	55	dep	12 eV	1082:1086	arg1	i.e.					1076:1079	i.e.	1076:1079	i.e.	1076:1079	Results indicate that at lower energy (i.e., 12 eV), the total signal intensity is one order of magnitude lower compared to the reference signal at 70 eV, but the ranges of variations for 2D peak responses is larger, extending the dynamic range.
33140127	1	56	theme	two-dimensional	287:301	arg1	chromatography					307:320	comprehensive two-dimensional gas chromatography	273:320	comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™)	273:440	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	1	57	theme	chromatography	307:320	arg1	potential					260:268	the information potential	244:268	the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals	244:528	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	7	58	theme	fused	1692:1696	arg1	data					1698:1701	fused data	1692:1701	fused data	1692:1701	Multivariate statistics, principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA) show quite good patient clustering, with total explained variance by the first two principal components (PCs) that increases from 54% at 70 eV to 59% at 12 eV and up to 71% for fused data.
33140127	9	59	attach	present	1891:1897	arg1	amount					1918:1923	higher relative amount	1902:1923	higher relative amount in saliva from MHO patients	1902:1951	Within the most informative analytes, lactose is present in higher relative amount in saliva from MHO patients, whereas N-acetyl-D-glucosamine, urea, glucuronic acid γ-lactone, 2-deoxyribose, N-acetylneuraminic acid methyl ester, and 5-aminovaleric acid are more abundant in MUO patients.
33140127	9	59	attach	present	1891:1897	arg2	lactose					1880:1886	lactose	1880:1886	lactose	1880:1886	Within the most informative analytes, lactose is present in higher relative amount in saliva from MHO patients, whereas N-acetyl-D-glucosamine, urea, glucuronic acid γ-lactone, 2-deoxyribose, N-acetylneuraminic acid methyl ester, and 5-aminovaleric acid are more abundant in MUO patients.
33140127	9	60	theme	acid	2053:2056	arg1	ester					2065:2069	N-acetylneuraminic acid methyl ester	2034:2069	N-acetylneuraminic acid methyl ester	2034:2069	Within the most informative analytes, lactose is present in higher relative amount in saliva from MHO patients, whereas N-acetyl-D-glucosamine, urea, glucuronic acid γ-lactone, 2-deoxyribose, N-acetylneuraminic acid methyl ester, and 5-aminovaleric acid are more abundant in MUO patients.
33140127	9	61	from	abundant	2105:2112	arg1	patients					2121:2128	MUO patients	2117:2128	MUO patients	2117:2128	Within the most informative analytes, lactose is present in higher relative amount in saliva from MHO patients, whereas N-acetyl-D-glucosamine, urea, glucuronic acid γ-lactone, 2-deoxyribose, N-acetylneuraminic acid methyl ester, and 5-aminovaleric acid are more abundant in MUO patients.
33140127	1	62	theme	small	497:501	arg1	group					503:507	a small group	495:507	a small group of obese individuals	495:528	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	1	62	theme	small	497:501	arg1	individuals					518:528	obese individuals	512:528	obese individuals	512:528	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	7	63	theme	component	1432:1440	arg1	PCA					1452:1454	PCA	1452:1454	PCA	1452:1454	Multivariate statistics, principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA) show quite good patient clustering, with total explained variance by the first two principal components (PCs) that increases from 54% at 70 eV to 59% at 12 eV and up to 71% for fused data.
33140127	7	63	theme	component	1432:1440	arg1	analysis					1442:1449	principal component analysis	1422:1449	principal component analysis (PCA)	1422:1455	Multivariate statistics, principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA) show quite good patient clustering, with total explained variance by the first two principal components (PCs) that increases from 54% at 70 eV to 59% at 12 eV and up to 71% for fused data.
33140127	4	64	theme	single	915:920	arg1	streams					927:933	single data streams	915:933	single data streams	915:933	Untargeted fingerprinting, based on pattern recognition by template matching, is applied on single data streams and on fused data, obtained by combining raw signals from the two ionization energies (12 and 70 eV).
33140127	8	65	theme	retention	1796:1804	arg1	data					1806:1809	retention data	1796:1809	retention data	1796:1809	With PLS-DA, discriminant components are highlighted and putatively identified by comparing retention data and 70 eV spectral signatures.
33140127	0	66	theme	metabolite	45:54	arg1	fingerprints					56:67	saliva metabolite fingerprints	38:67	saliva metabolite fingerprints from metabolically healthy and unhealthy obese patients	38:123	Exploring extra dimensions to capture saliva metabolite fingerprints from metabolically healthy and unhealthy obese patients by comprehensive two-dimensional gas chromatography featuring Tandem Ionization mass spectrometry.
33140127	1	67	theme	individuals	518:528	arg1	group					503:507	a small group	495:507	a small group of obese individuals	495:528	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	1	67	theme	individuals	518:528	arg1	individuals					518:528	obese individuals	512:528	obese individuals	512:528	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	10	68	theme	metabolite	2222:2231	arg1	variations					2233:2242	metabolite variations	2222:2242	metabolite variations between composite per-class images obtained by combining raw data from individuals belonging to different classes, i.e., MUO vs. MHO.Graphical abstract	2222:2394	Visual feature fingerprinting is combined with pattern recognition algorithms to highlight metabolite variations between composite per-class images obtained by combining raw data from individuals belonging to different classes, i.e., MUO vs. MHO.Graphical abstract.
33140127	5	69	from	70 eV	1185:1189	arg1	signal					1175:1180	the reference signal	1161:1180	the reference signal at 70 eV	1161:1189	Results indicate that at lower energy (i.e., 12 eV), the total signal intensity is one order of magnitude lower compared to the reference signal at 70 eV, but the ranges of variations for 2D peak responses is larger, extending the dynamic range.
33140127	7	70	from	12 eV	1668:1672	arg1	%					1663:1663	70 eV to 59%	1652:1663	70 eV to 59% at 12 eV	1652:1672	Multivariate statistics, principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA) show quite good patient clustering, with total explained variance by the first two principal components (PCs) that increases from 54% at 70 eV to 59% at 12 eV and up to 71% for fused data.
33140127	7	70	from	12 eV	1668:1672	arg1	%					1686:1686	up to 71%	1678:1686	up to 71% for fused data	1678:1701	Multivariate statistics, principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA) show quite good patient clustering, with total explained variance by the first two principal components (PCs) that increases from 54% at 70 eV to 59% at 12 eV and up to 71% for fused data.
33140127	6	71	theme	Fused	1283:1287	arg1	data					1289:1292	Fused data	1283:1292	Fused data	1283:1292	Fused data combine benefits from 70 eV and 12 eV resulting in more comprehensive coverage by sample fingerprints.
33140127	1	72	theme	information	248:258	arg1	potential					260:268	the information potential	244:268	the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals	244:528	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	2	73	theme	effective	576:584	arg1	exploitation					586:597	an effective exploitation	573:597	an effective exploitation of the complementary nature of tandem ionization data	573:651	The study presents a proof of concept for an effective exploitation of the complementary nature of tandem ionization data.
33140127	1	74	from	changes	451:457	arg1	group					503:507	a small group	495:507	a small group of obese individuals	495:528	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	1	74	from	changes	451:457	arg1	individuals					518:528	obese individuals	512:528	obese individuals	512:528	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	1	74	from	changes	451:457	arg1	signatures					479:488	saliva metabolic signatures	462:488	saliva metabolic signatures from a small group of obese individuals	462:528	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	4	75	dep	energies	1012:1019	arg1	70 eV					1029:1033	70 eV	1029:1033	70 eV	1029:1033	Untargeted fingerprinting, based on pattern recognition by template matching, is applied on single data streams and on fused data, obtained by combining raw signals from the two ionization energies (12 and 70 eV).
33140127	4	75	dep	energies	1012:1019	arg1	12					1022:1023	12	1022:1023	12	1022:1023	Untargeted fingerprinting, based on pattern recognition by template matching, is applied on single data streams and on fused data, obtained by combining raw signals from the two ionization energies (12 and 70 eV).
33140127	3	76	dep	obese	810:814	arg1	MUO					817:819	MUO	817:819	MUO	817:819	Samples are taken from two sub-populations of severely obese (BMI > 40 kg/m2) patients, named metabolically healthy obese (MHO) and metabolically unhealthy obese (MUO).
33140127	8	77	theme	spectral	1821:1828	arg1	signatures					1830:1839	70 eV spectral signatures	1815:1839	70 eV spectral signatures	1815:1839	With PLS-DA, discriminant components are highlighted and putatively identified by comparing retention data and 70 eV spectral signatures.
33140127	9	78	theme	acid	2003:2006	arg1	γ-lactone					2008:2016	glucuronic acid γ-lactone	1992:2016	glucuronic acid γ-lactone	1992:2016	Within the most informative analytes, lactose is present in higher relative amount in saliva from MHO patients, whereas N-acetyl-D-glucosamine, urea, glucuronic acid γ-lactone, 2-deoxyribose, N-acetylneuraminic acid methyl ester, and 5-aminovaleric acid are more abundant in MUO patients.
33140127	0	79	theme	mass	205:208	arg1	spectrometry					210:221	Tandem Ionization mass spectrometry	187:221	Tandem Ionization mass spectrometry	187:221	Exploring extra dimensions to capture saliva metabolite fingerprints from metabolically healthy and unhealthy obese patients by comprehensive two-dimensional gas chromatography featuring Tandem Ionization mass spectrometry.
33140127	1	80	theme	metabolic	469:477	arg1	signatures					479:488	saliva metabolic signatures	462:488	saliva metabolic signatures from a small group of obese individuals	462:528	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	2	81	theme	complementary	606:618	arg1	nature					620:625	the complementary nature	602:625	the complementary nature of tandem ionization data	602:651	The study presents a proof of concept for an effective exploitation of the complementary nature of tandem ionization data.
33140127	7	82	theme	patient	1531:1537	arg1	clustering					1539:1548	quite good patient clustering	1520:1548	quite good patient clustering	1520:1548	Multivariate statistics, principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA) show quite good patient clustering, with total explained variance by the first two principal components (PCs) that increases from 54% at 70 eV to 59% at 12 eV and up to 71% for fused data.
33140127	9	83	theme	higher	1902:1907	arg1	amount					1918:1923	higher relative amount	1902:1923	higher relative amount in saliva from MHO patients	1902:1951	Within the most informative analytes, lactose is present in higher relative amount in saliva from MHO patients, whereas N-acetyl-D-glucosamine, urea, glucuronic acid γ-lactone, 2-deoxyribose, N-acetylneuraminic acid methyl ester, and 5-aminovaleric acid are more abundant in MUO patients.
33140127	5	84	theme	variations	1210:1219	arg1	larger					1246:1251	larger	1246:1251	larger	1246:1251	Results indicate that at lower energy (i.e., 12 eV), the total signal intensity is one order of magnitude lower compared to the reference signal at 70 eV, but the ranges of variations for 2D peak responses is larger, extending the dynamic range.
33140127	5	84	theme	variations	1210:1219	arg1	ranges					1200:1205	the ranges	1196:1205	the ranges of variations for 2D peak responses	1196:1241	Results indicate that at lower energy (i.e., 12 eV), the total signal intensity is one order of magnitude lower compared to the reference signal at 70 eV, but the ranges of variations for 2D peak responses is larger, extending the dynamic range.
33140127	10	85	theme	recognition	2186:2196	arg1	algorithms					2198:2207	pattern recognition algorithms	2178:2207	pattern recognition algorithms	2178:2207	Visual feature fingerprinting is combined with pattern recognition algorithms to highlight metabolite variations between composite per-class images obtained by combining raw data from individuals belonging to different classes, i.e., MUO vs. MHO.Graphical abstract.
33140127	6	86	from	12 eV	1326:1330	arg1	benefits					1302:1309	benefits	1302:1309	benefits from 70 eV and 12 eV resulting in more comprehensive coverage by sample fingerprints	1302:1394	Fused data combine benefits from 70 eV and 12 eV resulting in more comprehensive coverage by sample fingerprints.
33140127	4	87	theme	ionization	1001:1010	arg1	energies					1012:1019	the two ionization energies	993:1019	the two ionization energies (12 and 70 eV)	993:1034	Untargeted fingerprinting, based on pattern recognition by template matching, is applied on single data streams and on fused data, obtained by combining raw signals from the two ionization energies (12 and 70 eV).
33140127	7	88	dep	59	1661:1662	arg1	to					1658:1659	to	1658:1659	to	1658:1659	Multivariate statistics, principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA) show quite good patient clustering, with total explained variance by the first two principal components (PCs) that increases from 54% at 70 eV to 59% at 12 eV and up to 71% for fused data.
33140127	5	89	theme	2D	1225:1226	arg1	responses					1233:1241	2D peak responses	1225:1241	2D peak responses	1225:1241	Results indicate that at lower energy (i.e., 12 eV), the total signal intensity is one order of magnitude lower compared to the reference signal at 70 eV, but the ranges of variations for 2D peak responses is larger, extending the dynamic range.
33140127	0	90	theme	obese	110:114	arg1	patients					116:123	metabolically healthy and unhealthy obese patients	74:123	metabolically healthy and unhealthy obese patients	74:123	Exploring extra dimensions to capture saliva metabolite fingerprints from metabolically healthy and unhealthy obese patients by comprehensive two-dimensional gas chromatography featuring Tandem Ionization mass spectrometry.
33140127	1	91	theme	i.e.	416:419	arg1	Ionization™					429:439	i.e., Tandem Ionization™	416:439	Ionization™	429:439	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	1	91	theme	i.e.	416:419	arg1	energy					408:413	variable ionization energy	388:413	variable ionization energy (i.e., Tandem Ionization™)	388:440	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	10	92	theme	Visual	2131:2136	arg1	fingerprinting					2146:2159	Visual feature fingerprinting	2131:2159	Visual feature fingerprinting	2131:2159	Visual feature fingerprinting is combined with pattern recognition algorithms to highlight metabolite variations between composite per-class images obtained by combining raw data from individuals belonging to different classes, i.e., MUO vs. MHO.Graphical abstract.
33140127	1	93	theme	Tandem	422:427	arg1	Ionization™					429:439	i.e., Tandem Ionization™	416:439	Ionization™	429:439	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	1	93	theme	Tandem	422:427	arg1	energy					408:413	variable ionization energy	388:413	variable ionization energy (i.e., Tandem Ionization™)	388:440	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	9	94	theme	MHO	1940:1942	arg1	patients					1944:1951	MHO patients	1940:1951	MHO patients	1940:1951	Within the most informative analytes, lactose is present in higher relative amount in saliva from MHO patients, whereas N-acetyl-D-glucosamine, urea, glucuronic acid γ-lactone, 2-deoxyribose, N-acetylneuraminic acid methyl ester, and 5-aminovaleric acid are more abundant in MUO patients.
33140127	0	95	theme	extra	10:14	arg1	dimensions					16:25	extra dimensions	10:25	extra dimensions	10:25	Exploring extra dimensions to capture saliva metabolite fingerprints from metabolically healthy and unhealthy obese patients by comprehensive two-dimensional gas chromatography featuring Tandem Ionization mass spectrometry.
33140127	5	96	theme	total	1094:1098	arg1	intensity					1107:1115	the total signal intensity	1090:1115	the total signal intensity	1090:1115	Results indicate that at lower energy (i.e., 12 eV), the total signal intensity is one order of magnitude lower compared to the reference signal at 70 eV, but the ranges of variations for 2D peak responses is larger, extending the dynamic range.
33140127	5	96	theme	total	1094:1098	arg1	lower					1143:1147	lower	1143:1147	lower	1143:1147	Results indicate that at lower energy (i.e., 12 eV), the total signal intensity is one order of magnitude lower compared to the reference signal at 70 eV, but the ranges of variations for 2D peak responses is larger, extending the dynamic range.
33140127	8	97	theme	discriminant	1717:1728	arg1	components					1730:1739	discriminant components	1717:1739	discriminant components	1717:1739	With PLS-DA, discriminant components are highlighted and putatively identified by comparing retention data and 70 eV spectral signatures.
33140127	9	98	from	present	1891:1897	arg1	amount					1918:1923	higher relative amount	1902:1923	higher relative amount in saliva from MHO patients	1902:1951	Within the most informative analytes, lactose is present in higher relative amount in saliva from MHO patients, whereas N-acetyl-D-glucosamine, urea, glucuronic acid γ-lactone, 2-deoxyribose, N-acetylneuraminic acid methyl ester, and 5-aminovaleric acid are more abundant in MUO patients.
33140127	10	99	theme	MHO.Graphical	2373:2385	arg1	abstract					2387:2394	MHO.Graphical abstract	2373:2394	MHO.Graphical abstract	2373:2394	Visual feature fingerprinting is combined with pattern recognition algorithms to highlight metabolite variations between composite per-class images obtained by combining raw data from individuals belonging to different classes, i.e., MUO vs. MHO.Graphical abstract.
33140127	5	100	theme	dynamic	1268:1274	arg1	range					1276:1280	the dynamic range	1264:1280	the dynamic range	1264:1280	Results indicate that at lower energy (i.e., 12 eV), the total signal intensity is one order of magnitude lower compared to the reference signal at 70 eV, but the ranges of variations for 2D peak responses is larger, extending the dynamic range.
33140127	6	101	theme	sample	1376:1381	arg1	fingerprints					1383:1394	sample fingerprints	1376:1394	sample fingerprints	1376:1394	Fused data combine benefits from 70 eV and 12 eV resulting in more comprehensive coverage by sample fingerprints.
33140127	3	102	dep	healthy	762:768	arg1	obese					810:814	obese	810:814	obese	810:814	Samples are taken from two sub-populations of severely obese (BMI > 40 kg/m2) patients, named metabolically healthy obese (MHO) and metabolically unhealthy obese (MUO).
33140127	3	102	dep	healthy	762:768	arg1	MHO					777:779	MHO	777:779	MHO	777:779	Samples are taken from two sub-populations of severely obese (BMI > 40 kg/m2) patients, named metabolically healthy obese (MHO) and metabolically unhealthy obese (MUO).
33140127	3	102	dep	healthy	762:768	arg1	obese					770:774	obese	770:774	obese	770:774	Samples are taken from two sub-populations of severely obese (BMI > 40 kg/m2) patients, named metabolically healthy obese (MHO) and metabolically unhealthy obese (MUO).
33140127	5	103	theme	reference	1165:1173	arg1	signal					1175:1180	the reference signal	1161:1180	the reference signal at 70 eV	1161:1189	Results indicate that at lower energy (i.e., 12 eV), the total signal intensity is one order of magnitude lower compared to the reference signal at 70 eV, but the ranges of variations for 2D peak responses is larger, extending the dynamic range.
33140127	9	104	located	present	1891:1897	arg1	analytes					1870:1877	the most informative analytes	1849:1877	the most informative analytes	1849:1877	Within the most informative analytes, lactose is present in higher relative amount in saliva from MHO patients, whereas N-acetyl-D-glucosamine, urea, glucuronic acid γ-lactone, 2-deoxyribose, N-acetylneuraminic acid methyl ester, and 5-aminovaleric acid are more abundant in MUO patients.
33140127	9	104	located	present	1891:1897	arg1	amount					1918:1923	higher relative amount	1902:1923	higher relative amount in saliva from MHO patients	1902:1951	Within the most informative analytes, lactose is present in higher relative amount in saliva from MHO patients, whereas N-acetyl-D-glucosamine, urea, glucuronic acid γ-lactone, 2-deoxyribose, N-acetylneuraminic acid methyl ester, and 5-aminovaleric acid are more abundant in MUO patients.
33140127	9	104	located	present	1891:1897	arg2	lactose					1880:1886	lactose	1880:1886	lactose	1880:1886	Within the most informative analytes, lactose is present in higher relative amount in saliva from MHO patients, whereas N-acetyl-D-glucosamine, urea, glucuronic acid γ-lactone, 2-deoxyribose, N-acetylneuraminic acid methyl ester, and 5-aminovaleric acid are more abundant in MUO patients.
33140127	10	105	theme	pattern	2178:2184	arg1	algorithms					2198:2207	pattern recognition algorithms	2178:2207	pattern recognition algorithms	2178:2207	Visual feature fingerprinting is combined with pattern recognition algorithms to highlight metabolite variations between composite per-class images obtained by combining raw data from individuals belonging to different classes, i.e., MUO vs. MHO.Graphical abstract.
33140127	10	106	theme	per-class	2262:2270	arg1	images					2272:2277	composite per-class images	2252:2277	composite per-class images obtained by combining raw data from individuals belonging to different classes, i.e., MUO vs. MHO.Graphical abstract	2252:2394	Visual feature fingerprinting is combined with pattern recognition algorithms to highlight metabolite variations between composite per-class images obtained by combining raw data from individuals belonging to different classes, i.e., MUO vs. MHO.Graphical abstract.
33140127	9	107	theme	glucuronic	1992:2001	arg1	γ-lactone					2008:2016	glucuronic acid γ-lactone	1992:2016	glucuronic acid γ-lactone	1992:2016	Within the most informative analytes, lactose is present in higher relative amount in saliva from MHO patients, whereas N-acetyl-D-glucosamine, urea, glucuronic acid γ-lactone, 2-deoxyribose, N-acetylneuraminic acid methyl ester, and 5-aminovaleric acid are more abundant in MUO patients.
33140127	10	108	dep	MUO	2365:2367	arg1	i.e.					2359:2362	i.e.	2359:2362	i.e.	2359:2362	Visual feature fingerprinting is combined with pattern recognition algorithms to highlight metabolite variations between composite per-class images obtained by combining raw data from individuals belonging to different classes, i.e., MUO vs. MHO.Graphical abstract.
33140127	1	109	theme	variable	388:395	arg1	Ionization™					429:439	i.e., Tandem Ionization™	416:439	Ionization™	429:439	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	1	109	theme	variable	388:395	arg1	energy					408:413	variable ionization energy	388:413	variable ionization energy (i.e., Tandem Ionization™)	388:440	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	7	110	theme	squares	1476:1482	arg1	analysis					1497:1504	partial least squares discriminant analysis	1462:1504	partial least squares discriminant analysis (PLS-DA)	1462:1513	Multivariate statistics, principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA) show quite good patient clustering, with total explained variance by the first two principal components (PCs) that increases from 54% at 70 eV to 59% at 12 eV and up to 71% for fused data.
33140127	7	110	theme	squares	1476:1482	arg1	PLS-DA					1507:1512	PLS-DA	1507:1512	PLS-DA	1507:1512	Multivariate statistics, principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA) show quite good patient clustering, with total explained variance by the first two principal components (PCs) that increases from 54% at 70 eV to 59% at 12 eV and up to 71% for fused data.
33140127	4	111	theme	Untargeted	823:832	arg1	fingerprinting					834:847	Untargeted fingerprinting	823:847	Untargeted fingerprinting	823:847	Untargeted fingerprinting, based on pattern recognition by template matching, is applied on single data streams and on fused data, obtained by combining raw signals from the two ionization energies (12 and 70 eV).
33140127	1	112	theme	comprehensive	273:285	arg1	chromatography					307:320	comprehensive two-dimensional gas chromatography	273:320	comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™)	273:440	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	7	113	theme	partial	1462:1468	arg1	analysis					1497:1504	partial least squares discriminant analysis	1462:1504	partial least squares discriminant analysis (PLS-DA)	1462:1513	Multivariate statistics, principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA) show quite good patient clustering, with total explained variance by the first two principal components (PCs) that increases from 54% at 70 eV to 59% at 12 eV and up to 71% for fused data.
33140127	7	113	theme	partial	1462:1468	arg1	PLS-DA					1507:1512	PLS-DA	1507:1512	PLS-DA	1507:1512	Multivariate statistics, principal component analysis (PCA), and partial least squares discriminant analysis (PLS-DA) show quite good patient clustering, with total explained variance by the first two principal components (PCs) that increases from 54% at 70 eV to 59% at 12 eV and up to 71% for fused data.
33140127	1	114	from	group	503:507	arg1	changes					451:457	changes	451:457	changes in saliva metabolic signatures from a small group of obese individuals	451:528	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	1	114	from	group	503:507	arg1	signatures					479:488	saliva metabolic signatures	462:488	saliva metabolic signatures from a small group of obese individuals	462:528	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
33140127	1	115	theme	gas	303:305	arg1	chromatography					307:320	comprehensive two-dimensional gas chromatography	273:320	comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™)	273:440	This study examines the information potential of comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC×GC-TOF MS) and variable ionization energy (i.e., Tandem Ionization™) to study changes in saliva metabolic signatures from a small group of obese individuals.
32747289	7	0	from	H2O2	1058:1061	arg1	selectivity					1183:1193	high selectivity	1178:1193	high selectivity	1178:1193	Wide linearity from 5 to 1000 μM H2O2, with low limit of detection of 1.0 μM in addition to satisfactory precision (< 3.5 %) and recovery (95-105 %) and high selectivity was achieved.
32747289	7	0	from	H2O2	1058:1061	arg1	linearity					1030:1038	Wide linearity	1025:1038	Wide linearity	1025:1038	Wide linearity from 5 to 1000 μM H2O2, with low limit of detection of 1.0 μM in addition to satisfactory precision (< 3.5 %) and recovery (95-105 %) and high selectivity was achieved.
32747289	1	1	theme	silver/poly	138:148	arg1	PABA-SA					206:212	Ag@PABA-SA	203:212	Ag@PABA-SA	203:212	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel (Ag@PABA-SA) was fabricated through green, in situ chemical oxidative polymerization method.
32747289	1	1	theme	silver/poly	138:148	arg1	nanogel					194:200	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel	134:200	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel (Ag@PABA-SA)	134:213	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel (Ag@PABA-SA) was fabricated through green, in situ chemical oxidative polymerization method.
32747289	5	2	theme	FTIR	852:855	arg1	TGA					926:928	TGA	926:928	TGA	926:928	The morphology, size, surface charge, composition and thermal stability of nanogel was characterized by UV-vis, Raman and FTIR spectroscopy, TEM, FESEM, EDX, XRD, DLS and zeta potential analysis, TGA and DTG.
32747289	5	2	theme	FTIR	852:855	arg1	DTG					934:936	DTG	934:936	DTG	934:936	The morphology, size, surface charge, composition and thermal stability of nanogel was characterized by UV-vis, Raman and FTIR spectroscopy, TEM, FESEM, EDX, XRD, DLS and zeta potential analysis, TGA and DTG.
32747289	5	2	theme	FTIR	852:855	arg1	spectroscopy					857:868	UV-vis, Raman and FTIR spectroscopy	834:868	spectroscopy	857:868	The morphology, size, surface charge, composition and thermal stability of nanogel was characterized by UV-vis, Raman and FTIR spectroscopy, TEM, FESEM, EDX, XRD, DLS and zeta potential analysis, TGA and DTG.
32747289	7	3	dep	1000	1050:1053	arg1	to					1047:1048	to	1047:1048	to	1047:1048	Wide linearity from 5 to 1000 μM H2O2, with low limit of detection of 1.0 μM in addition to satisfactory precision (< 3.5 %) and recovery (95-105 %) and high selectivity was achieved.
32747289	5	4	theme	Raman	842:846	arg1	TGA					926:928	TGA	926:928	TGA	926:928	The morphology, size, surface charge, composition and thermal stability of nanogel was characterized by UV-vis, Raman and FTIR spectroscopy, TEM, FESEM, EDX, XRD, DLS and zeta potential analysis, TGA and DTG.
32747289	5	4	theme	Raman	842:846	arg1	DTG					934:936	DTG	934:936	DTG	934:936	The morphology, size, surface charge, composition and thermal stability of nanogel was characterized by UV-vis, Raman and FTIR spectroscopy, TEM, FESEM, EDX, XRD, DLS and zeta potential analysis, TGA and DTG.
32747289	5	4	theme	Raman	842:846	arg1	spectroscopy					857:868	UV-vis, Raman and FTIR spectroscopy	834:868	spectroscopy	857:868	The morphology, size, surface charge, composition and thermal stability of nanogel was characterized by UV-vis, Raman and FTIR spectroscopy, TEM, FESEM, EDX, XRD, DLS and zeta potential analysis, TGA and DTG.
32747289	0	5	theme	alginate	77:84	arg1	nanogel					86:92	silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel	30:92	silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel	30:92	Green, in situ fabrication of silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel and hydrogen peroxide sensing capacity.
32747289	1	6	dep	in	245:246	arg1	situ					248:251	situ	248:251	situ	248:251	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel (Ag@PABA-SA) was fabricated through green, in situ chemical oxidative polymerization method.
32747289	7	7	dep	μM	1055:1056	arg1	1000					1050:1053	1000	1050:1053	1000	1050:1053	Wide linearity from 5 to 1000 μM H2O2, with low limit of detection of 1.0 μM in addition to satisfactory precision (< 3.5 %) and recovery (95-105 %) and high selectivity was achieved.
32747289	4	8	theme	based	717:721	arg1	AgNPs					723:727	highly stable, dispersed polymer based AgNPs	684:727	highly stable, dispersed polymer based AgNPs	684:727	This results highly stable, dispersed polymer based AgNPs.
32747289	0	9	theme	/sodium	69:75	arg1	nanogel					86:92	silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel	30:92	silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel	30:92	Green, in situ fabrication of silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel and hydrogen peroxide sensing capacity.
32747289	4	10	theme	polymer	709:715	arg1	AgNPs					723:727	highly stable, dispersed polymer based AgNPs	684:727	highly stable, dispersed polymer based AgNPs	684:727	This results highly stable, dispersed polymer based AgNPs.
32747289	3	11	theme	SA	498:499	arg1	present					501:507	SA present	498:507	SA present	498:507	Importantly with SA present, PABA will bind to hydroxyl groups of SA through covalent bonding to generate PABA-SA semi-interpenetrating network, on which AgNPs were concomitantly deposited.
32747289	4	12	theme	stable	691:696	arg1	AgNPs					723:727	highly stable, dispersed polymer based AgNPs	684:727	highly stable, dispersed polymer based AgNPs	684:727	This results highly stable, dispersed polymer based AgNPs.
32747289	6	13	theme	PABA-SA	946:952	arg1	probe					995:999	colorimetric probe	982:999	colorimetric probe towards H2O2 detection	982:1022	The Ag@PABA-SA nanogel was investigated as colorimetric probe towards H2O2 detection.
32747289	6	13	theme	PABA-SA	946:952	arg1	nanogel					954:960	The Ag@PABA-SA nanogel	939:960	The Ag@PABA-SA nanogel	939:960	The Ag@PABA-SA nanogel was investigated as colorimetric probe towards H2O2 detection.
32747289	1	14	theme	3-aminophenyl	150:162	arg1	acid					172:175	3-aminophenyl boronic acid	150:175	3-aminophenyl boronic acid	150:175	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel (Ag@PABA-SA) was fabricated through green, in situ chemical oxidative polymerization method.
32747289	0	15	theme	nanogel	86:92	arg1	Green					0:4	Green	0:4	Green	0:4	Green, in situ fabrication of silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel and hydrogen peroxide sensing capacity.
32747289	0	15	theme	nanogel	86:92	arg1	capacity					124:131	hydrogen peroxide sensing capacity	98:131	hydrogen peroxide sensing capacity	98:131	Green, in situ fabrication of silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel and hydrogen peroxide sensing capacity.
32747289	0	15	theme	nanogel	86:92	arg1	fabrication					15:25	in situ fabrication	7:25	in situ fabrication of silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel	7:92	Green, in situ fabrication of silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel and hydrogen peroxide sensing capacity.
32747289	6	16	theme	H2O2	1009:1012	arg1	detection					1014:1022	H2O2 detection	1009:1022	H2O2 detection	1009:1022	The Ag@PABA-SA nanogel was investigated as colorimetric probe towards H2O2 detection.
32747289	7	17	theme	low	1069:1071	arg1	limit					1073:1077	low limit	1069:1077	low limit of detection of 1.0 μM in addition to satisfactory precision (< 3.5 %) and recovery (95-105 %)	1069:1172	Wide linearity from 5 to 1000 μM H2O2, with low limit of detection of 1.0 μM in addition to satisfactory precision (< 3.5 %) and recovery (95-105 %) and high selectivity was achieved.
32747289	7	18	dep	precision	1130:1138	arg1	addition					1105:1112	addition	1105:1112	addition	1105:1112	Wide linearity from 5 to 1000 μM H2O2, with low limit of detection of 1.0 μM in addition to satisfactory precision (< 3.5 %) and recovery (95-105 %) and high selectivity was achieved.
32747289	1	19	theme	boronic	164:170	arg1	acid					172:175	3-aminophenyl boronic acid	150:175	3-aminophenyl boronic acid	150:175	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel (Ag@PABA-SA) was fabricated through green, in situ chemical oxidative polymerization method.
32747289	7	20	theme	detection	1082:1090	arg1	limit					1073:1077	low limit	1069:1077	low limit of detection of 1.0 μM in addition to satisfactory precision (< 3.5 %) and recovery (95-105 %)	1069:1172	Wide linearity from 5 to 1000 μM H2O2, with low limit of detection of 1.0 μM in addition to satisfactory precision (< 3.5 %) and recovery (95-105 %) and high selectivity was achieved.
32747289	2	21	theme	polymeric	458:466	arg1	PABA					474:477	PABA	474:477	PABA	474:477	3-Aminophenyl boronic acid (APBA) served as the room temperature reductant of AgNO3 which acted as an oxidant towards the polymerization of APBA to its conducting polymeric form (PABA).
32747289	2	21	theme	polymeric	458:466	arg1	form					468:471	its conducting polymeric form	443:471	its conducting polymeric form (PABA)	443:478	3-Aminophenyl boronic acid (APBA) served as the room temperature reductant of AgNO3 which acted as an oxidant towards the polymerization of APBA to its conducting polymeric form (PABA).
32747289	1	22	dep	silver/poly	138:148	arg1	acid					172:175	3-aminophenyl boronic acid	150:175	3-aminophenyl boronic acid	150:175	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel (Ag@PABA-SA) was fabricated through green, in situ chemical oxidative polymerization method.
32747289	2	23	theme	room	343:346	arg1	temperature					348:358	the room temperature	339:358	the room temperature reductant of AgNO3 which acted as an oxidant towards the polymerization of APBA to its conducting polymeric form (PABA)	339:478	3-Aminophenyl boronic acid (APBA) served as the room temperature reductant of AgNO3 which acted as an oxidant towards the polymerization of APBA to its conducting polymeric form (PABA).
32747289	5	24	theme	surface	752:758	arg1	charge					760:765	surface charge	752:765	surface charge	752:765	The morphology, size, surface charge, composition and thermal stability of nanogel was characterized by UV-vis, Raman and FTIR spectroscopy, TEM, FESEM, EDX, XRD, DLS and zeta potential analysis, TGA and DTG.
32747289	5	25	dep	morphology	734:743	arg1	The					730:732	The	730:732	The	730:732	The morphology, size, surface charge, composition and thermal stability of nanogel was characterized by UV-vis, Raman and FTIR spectroscopy, TEM, FESEM, EDX, XRD, DLS and zeta potential analysis, TGA and DTG.
32747289	5	26	theme	nanogel	805:811	arg1	composition					768:778	composition	768:778	composition	768:778	The morphology, size, surface charge, composition and thermal stability of nanogel was characterized by UV-vis, Raman and FTIR spectroscopy, TEM, FESEM, EDX, XRD, DLS and zeta potential analysis, TGA and DTG.
32747289	5	26	theme	nanogel	805:811	arg1	stability					792:800	thermal stability	784:800	thermal stability	784:800	The morphology, size, surface charge, composition and thermal stability of nanogel was characterized by UV-vis, Raman and FTIR spectroscopy, TEM, FESEM, EDX, XRD, DLS and zeta potential analysis, TGA and DTG.
32747289	5	26	theme	nanogel	805:811	arg1	charge					760:765	surface charge	752:765	surface charge	752:765	The morphology, size, surface charge, composition and thermal stability of nanogel was characterized by UV-vis, Raman and FTIR spectroscopy, TEM, FESEM, EDX, XRD, DLS and zeta potential analysis, TGA and DTG.
32747289	5	26	theme	nanogel	805:811	arg1	morphology					734:743	morphology	734:743	morphology	734:743	The morphology, size, surface charge, composition and thermal stability of nanogel was characterized by UV-vis, Raman and FTIR spectroscopy, TEM, FESEM, EDX, XRD, DLS and zeta potential analysis, TGA and DTG.
32747289	5	26	theme	nanogel	805:811	arg1	size					746:749	size	746:749	size	746:749	The morphology, size, surface charge, composition and thermal stability of nanogel was characterized by UV-vis, Raman and FTIR spectroscopy, TEM, FESEM, EDX, XRD, DLS and zeta potential analysis, TGA and DTG.
32747289	6	27	theme	@	945:945	arg1	probe					995:999	colorimetric probe	982:999	colorimetric probe towards H2O2 detection	982:1022	The Ag@PABA-SA nanogel was investigated as colorimetric probe towards H2O2 detection.
32747289	6	27	theme	@	945:945	arg1	nanogel					954:960	The Ag@PABA-SA nanogel	939:960	The Ag@PABA-SA nanogel	939:960	The Ag@PABA-SA nanogel was investigated as colorimetric probe towards H2O2 detection.
32747289	5	28	theme	thermal	784:790	arg1	stability					792:800	thermal stability	784:800	thermal stability	784:800	The morphology, size, surface charge, composition and thermal stability of nanogel was characterized by UV-vis, Raman and FTIR spectroscopy, TEM, FESEM, EDX, XRD, DLS and zeta potential analysis, TGA and DTG.
32747289	1	29	theme	in	245:246	arg1	method					287:292	in situ chemical oxidative polymerization method	245:292	in situ chemical oxidative polymerization method	245:292	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel (Ag@PABA-SA) was fabricated through green, in situ chemical oxidative polymerization method.
32747289	1	29	theme	in	245:246	arg1	green					238:242	green	238:242	green	238:242	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel (Ag@PABA-SA) was fabricated through green, in situ chemical oxidative polymerization method.
32747289	0	30	theme	peroxide	107:114	arg1	Green					0:4	Green	0:4	Green	0:4	Green, in situ fabrication of silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel and hydrogen peroxide sensing capacity.
32747289	0	30	theme	peroxide	107:114	arg1	capacity					124:131	hydrogen peroxide sensing capacity	98:131	hydrogen peroxide sensing capacity	98:131	Green, in situ fabrication of silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel and hydrogen peroxide sensing capacity.
32747289	7	31	with	selectivity	1183:1193	arg1	limit					1073:1077	low limit	1069:1077	low limit of detection of 1.0 μM in addition to satisfactory precision (< 3.5 %) and recovery (95-105 %)	1069:1172	Wide linearity from 5 to 1000 μM H2O2, with low limit of detection of 1.0 μM in addition to satisfactory precision (< 3.5 %) and recovery (95-105 %) and high selectivity was achieved.
32747289	1	32	theme	/sodium	177:183	arg1	PABA-SA					206:212	Ag@PABA-SA	203:212	Ag@PABA-SA	203:212	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel (Ag@PABA-SA) was fabricated through green, in situ chemical oxidative polymerization method.
32747289	1	32	theme	/sodium	177:183	arg1	nanogel					194:200	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel	134:200	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel (Ag@PABA-SA)	134:213	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel (Ag@PABA-SA) was fabricated through green, in situ chemical oxidative polymerization method.
32747289	0	33	theme	in	7:8	arg1	Green					0:4	Green	0:4	Green	0:4	Green, in situ fabrication of silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel and hydrogen peroxide sensing capacity.
32747289	0	33	theme	in	7:8	arg1	fabrication					15:25	in situ fabrication	7:25	in situ fabrication of silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel	7:92	Green, in situ fabrication of silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel and hydrogen peroxide sensing capacity.
32747289	0	34	theme	hydrogen	98:105	arg1	peroxide					107:114	hydrogen peroxide	98:114	hydrogen peroxide sensing capacity	98:131	Green, in situ fabrication of silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel and hydrogen peroxide sensing capacity.
32747289	7	35	theme	μM	1099:1100	arg1	detection					1082:1090	detection	1082:1090	detection of 1.0 μM in addition to satisfactory precision (< 3.5 %) and recovery (95-105 %)	1082:1172	Wide linearity from 5 to 1000 μM H2O2, with low limit of detection of 1.0 μM in addition to satisfactory precision (< 3.5 %) and recovery (95-105 %) and high selectivity was achieved.
32747289	1	36	theme	alginate	185:192	arg1	PABA-SA					206:212	Ag@PABA-SA	203:212	Ag@PABA-SA	203:212	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel (Ag@PABA-SA) was fabricated through green, in situ chemical oxidative polymerization method.
32747289	1	36	theme	alginate	185:192	arg1	nanogel					194:200	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel	134:200	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel (Ag@PABA-SA)	134:213	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel (Ag@PABA-SA) was fabricated through green, in situ chemical oxidative polymerization method.
32747289	2	37	theme	APBA	435:438	arg1	polymerization					417:430	the polymerization	413:430	the polymerization of APBA to its conducting polymeric form (PABA)	413:478	3-Aminophenyl boronic acid (APBA) served as the room temperature reductant of AgNO3 which acted as an oxidant towards the polymerization of APBA to its conducting polymeric form (PABA).
32747289	1	38	theme	chemical	253:260	arg1	method					287:292	in situ chemical oxidative polymerization method	245:292	in situ chemical oxidative polymerization method	245:292	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel (Ag@PABA-SA) was fabricated through green, in situ chemical oxidative polymerization method.
32747289	1	38	theme	chemical	253:260	arg1	green					238:242	green	238:242	green	238:242	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel (Ag@PABA-SA) was fabricated through green, in situ chemical oxidative polymerization method.
32747289	3	39	theme	PABA-SA	587:593	arg1	network					617:623	PABA-SA semi-interpenetrating network	587:623	PABA-SA semi-interpenetrating network	587:623	Importantly with SA present, PABA will bind to hydroxyl groups of SA through covalent bonding to generate PABA-SA semi-interpenetrating network, on which AgNPs were concomitantly deposited.
32747289	3	40	theme	hydroxyl	528:535	arg1	SA					547:548	SA	547:548	SA	547:548	Importantly with SA present, PABA will bind to hydroxyl groups of SA through covalent bonding to generate PABA-SA semi-interpenetrating network, on which AgNPs were concomitantly deposited.
32747289	3	40	theme	hydroxyl	528:535	arg1	groups					537:542	hydroxyl groups	528:542	hydroxyl groups of SA	528:548	Importantly with SA present, PABA will bind to hydroxyl groups of SA through covalent bonding to generate PABA-SA semi-interpenetrating network, on which AgNPs were concomitantly deposited.
32747289	2	41	theme	AgNO3	373:377	arg1	reductant					360:368	the room temperature reductant	339:368	the room temperature reductant of AgNO3 which acted as an oxidant towards the polymerization of APBA to its conducting polymeric form (PABA)	339:478	3-Aminophenyl boronic acid (APBA) served as the room temperature reductant of AgNO3 which acted as an oxidant towards the polymerization of APBA to its conducting polymeric form (PABA).
32747289	1	42	theme	oxidative	262:270	arg1	method					287:292	in situ chemical oxidative polymerization method	245:292	in situ chemical oxidative polymerization method	245:292	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel (Ag@PABA-SA) was fabricated through green, in situ chemical oxidative polymerization method.
32747289	1	42	theme	oxidative	262:270	arg1	green					238:242	green	238:242	green	238:242	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel (Ag@PABA-SA) was fabricated through green, in situ chemical oxidative polymerization method.
32747289	0	43	theme	sensing	116:122	arg1	Green					0:4	Green	0:4	Green	0:4	Green, in situ fabrication of silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel and hydrogen peroxide sensing capacity.
32747289	0	43	theme	sensing	116:122	arg1	capacity					124:131	hydrogen peroxide sensing capacity	98:131	hydrogen peroxide sensing capacity	98:131	Green, in situ fabrication of silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel and hydrogen peroxide sensing capacity.
32747289	0	44	dep	silver/poly	30:40	arg1	acid					64:67	3-aminophenyl boronic acid	42:67	3-aminophenyl boronic acid	42:67	Green, in situ fabrication of silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel and hydrogen peroxide sensing capacity.
32747289	7	45	theme	Wide	1025:1028	arg1	linearity					1030:1038	Wide linearity	1025:1038	Wide linearity	1025:1038	Wide linearity from 5 to 1000 μM H2O2, with low limit of detection of 1.0 μM in addition to satisfactory precision (< 3.5 %) and recovery (95-105 %) and high selectivity was achieved.
32747289	1	46	theme	polymerization	272:285	arg1	method					287:292	in situ chemical oxidative polymerization method	245:292	in situ chemical oxidative polymerization method	245:292	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel (Ag@PABA-SA) was fabricated through green, in situ chemical oxidative polymerization method.
32747289	1	46	theme	polymerization	272:285	arg1	green					238:242	green	238:242	green	238:242	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel (Ag@PABA-SA) was fabricated through green, in situ chemical oxidative polymerization method.
32747289	2	47	theme	conducting	447:456	arg1	PABA					474:477	PABA	474:477	PABA	474:477	3-Aminophenyl boronic acid (APBA) served as the room temperature reductant of AgNO3 which acted as an oxidant towards the polymerization of APBA to its conducting polymeric form (PABA).
32747289	2	47	theme	conducting	447:456	arg1	form					468:471	its conducting polymeric form	443:471	its conducting polymeric form (PABA)	443:478	3-Aminophenyl boronic acid (APBA) served as the room temperature reductant of AgNO3 which acted as an oxidant towards the polymerization of APBA to its conducting polymeric form (PABA).
32747289	3	48	theme	covalent	558:565	arg1	bonding					567:573	covalent bonding	558:573	covalent bonding	558:573	Importantly with SA present, PABA will bind to hydroxyl groups of SA through covalent bonding to generate PABA-SA semi-interpenetrating network, on which AgNPs were concomitantly deposited.
32747289	0	49	theme	silver/poly	30:40	arg1	nanogel					86:92	silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel	30:92	silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel	30:92	Green, in situ fabrication of silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel and hydrogen peroxide sensing capacity.
32747289	7	50	with	linearity	1030:1038	arg1	limit					1073:1077	low limit	1069:1077	low limit of detection of 1.0 μM in addition to satisfactory precision (< 3.5 %) and recovery (95-105 %)	1069:1172	Wide linearity from 5 to 1000 μM H2O2, with low limit of detection of 1.0 μM in addition to satisfactory precision (< 3.5 %) and recovery (95-105 %) and high selectivity was achieved.
32747289	4	51	dep	stable	691:696	arg1	dispersed					699:707	dispersed	699:707	dispersed	699:707	This results highly stable, dispersed polymer based AgNPs.
32747289	5	52	theme	potential	906:914	arg1	analysis					916:923	zeta potential analysis	901:923	zeta potential analysis	901:923	The morphology, size, surface charge, composition and thermal stability of nanogel was characterized by UV-vis, Raman and FTIR spectroscopy, TEM, FESEM, EDX, XRD, DLS and zeta potential analysis, TGA and DTG.
32747289	7	53	dep	H2O2	1058:1061	arg1	μM					1055:1056	μM	1055:1056	μM	1055:1056	Wide linearity from 5 to 1000 μM H2O2, with low limit of detection of 1.0 μM in addition to satisfactory precision (< 3.5 %) and recovery (95-105 %) and high selectivity was achieved.
32747289	7	54	theme	high	1178:1181	arg1	selectivity					1183:1193	high selectivity	1178:1193	high selectivity	1178:1193	Wide linearity from 5 to 1000 μM H2O2, with low limit of detection of 1.0 μM in addition to satisfactory precision (< 3.5 %) and recovery (95-105 %) and high selectivity was achieved.
32747289	2	55	theme	boronic	309:315	arg1	acid					317:320	3-Aminophenyl boronic acid	295:320	3-Aminophenyl boronic acid (APBA)	295:327	3-Aminophenyl boronic acid (APBA) served as the room temperature reductant of AgNO3 which acted as an oxidant towards the polymerization of APBA to its conducting polymeric form (PABA).
32747289	2	55	theme	boronic	309:315	arg1	APBA					323:326	APBA	323:326	APBA	323:326	3-Aminophenyl boronic acid (APBA) served as the room temperature reductant of AgNO3 which acted as an oxidant towards the polymerization of APBA to its conducting polymeric form (PABA).
32747289	1	56	theme	Ag	203:204	arg1	PABA-SA					206:212	Ag@PABA-SA	203:212	Ag@PABA-SA	203:212	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel (Ag@PABA-SA) was fabricated through green, in situ chemical oxidative polymerization method.
32747289	1	56	theme	Ag	203:204	arg1	nanogel					194:200	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel	134:200	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel (Ag@PABA-SA)	134:213	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel (Ag@PABA-SA) was fabricated through green, in situ chemical oxidative polymerization method.
32747289	3	57	theme	semi-interpenetrating	595:615	arg1	network					617:623	PABA-SA semi-interpenetrating network	587:623	PABA-SA semi-interpenetrating network	587:623	Importantly with SA present, PABA will bind to hydroxyl groups of SA through covalent bonding to generate PABA-SA semi-interpenetrating network, on which AgNPs were concomitantly deposited.
32747289	0	58	dep	in	7:8	arg1	situ					10:13	situ	10:13	situ	10:13	Green, in situ fabrication of silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel and hydrogen peroxide sensing capacity.
32747289	2	59	theme	3-Aminophenyl	295:307	arg1	acid					317:320	3-Aminophenyl boronic acid	295:320	3-Aminophenyl boronic acid (APBA)	295:327	3-Aminophenyl boronic acid (APBA) served as the room temperature reductant of AgNO3 which acted as an oxidant towards the polymerization of APBA to its conducting polymeric form (PABA).
32747289	2	59	theme	3-Aminophenyl	295:307	arg1	APBA					323:326	APBA	323:326	APBA	323:326	3-Aminophenyl boronic acid (APBA) served as the room temperature reductant of AgNO3 which acted as an oxidant towards the polymerization of APBA to its conducting polymeric form (PABA).
32747289	1	60	theme	@	205:205	arg1	PABA-SA					206:212	Ag@PABA-SA	203:212	Ag@PABA-SA	203:212	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel (Ag@PABA-SA) was fabricated through green, in situ chemical oxidative polymerization method.
32747289	1	60	theme	@	205:205	arg1	nanogel					194:200	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel	134:200	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel (Ag@PABA-SA)	134:213	The silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel (Ag@PABA-SA) was fabricated through green, in situ chemical oxidative polymerization method.
32747289	0	61	theme	boronic	56:62	arg1	acid					64:67	3-aminophenyl boronic acid	42:67	3-aminophenyl boronic acid	42:67	Green, in situ fabrication of silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel and hydrogen peroxide sensing capacity.
32747289	6	62	theme	colorimetric	982:993	arg1	probe					995:999	colorimetric probe	982:999	colorimetric probe towards H2O2 detection	982:1022	The Ag@PABA-SA nanogel was investigated as colorimetric probe towards H2O2 detection.
32747289	6	62	theme	colorimetric	982:993	arg1	nanogel					954:960	The Ag@PABA-SA nanogel	939:960	The Ag@PABA-SA nanogel	939:960	The Ag@PABA-SA nanogel was investigated as colorimetric probe towards H2O2 detection.
32747289	5	63	theme	zeta	901:904	arg1	analysis					916:923	zeta potential analysis	901:923	zeta potential analysis	901:923	The morphology, size, surface charge, composition and thermal stability of nanogel was characterized by UV-vis, Raman and FTIR spectroscopy, TEM, FESEM, EDX, XRD, DLS and zeta potential analysis, TGA and DTG.
32747289	6	64	theme	Ag	943:944	arg1	probe					995:999	colorimetric probe	982:999	colorimetric probe towards H2O2 detection	982:1022	The Ag@PABA-SA nanogel was investigated as colorimetric probe towards H2O2 detection.
32747289	6	64	theme	Ag	943:944	arg1	nanogel					954:960	The Ag@PABA-SA nanogel	939:960	The Ag@PABA-SA nanogel	939:960	The Ag@PABA-SA nanogel was investigated as colorimetric probe towards H2O2 detection.
32747289	2	65	theme	temperature	348:358	arg1	reductant					360:368	the room temperature reductant	339:368	the room temperature reductant of AgNO3 which acted as an oxidant towards the polymerization of APBA to its conducting polymeric form (PABA)	339:478	3-Aminophenyl boronic acid (APBA) served as the room temperature reductant of AgNO3 which acted as an oxidant towards the polymerization of APBA to its conducting polymeric form (PABA).
32747289	0	66	theme	3-aminophenyl	42:54	arg1	acid					64:67	3-aminophenyl boronic acid	42:67	3-aminophenyl boronic acid	42:67	Green, in situ fabrication of silver/poly(3-aminophenyl boronic acid)/sodium alginate nanogel and hydrogen peroxide sensing capacity.
32747289	3	67	theme	SA	547:548	arg1	SA					547:548	SA	547:548	SA	547:548	Importantly with SA present, PABA will bind to hydroxyl groups of SA through covalent bonding to generate PABA-SA semi-interpenetrating network, on which AgNPs were concomitantly deposited.
32747289	3	67	theme	SA	547:548	arg1	groups					537:542	hydroxyl groups	528:542	hydroxyl groups of SA	528:548	Importantly with SA present, PABA will bind to hydroxyl groups of SA through covalent bonding to generate PABA-SA semi-interpenetrating network, on which AgNPs were concomitantly deposited.
32747289	7	68	theme	satisfactory	1117:1128	arg1	precision					1130:1138	satisfactory precision	1117:1138	satisfactory precision (< 3.5 %)	1117:1148	Wide linearity from 5 to 1000 μM H2O2, with low limit of detection of 1.0 μM in addition to satisfactory precision (< 3.5 %) and recovery (95-105 %) and high selectivity was achieved.
32747289	7	68	theme	satisfactory	1117:1128	arg1	%					1147:1147	< 3.5 %	1141:1147	< 3.5 %	1141:1147	Wide linearity from 5 to 1000 μM H2O2, with low limit of detection of 1.0 μM in addition to satisfactory precision (< 3.5 %) and recovery (95-105 %) and high selectivity was achieved.
32747289	5	69	theme	UV-vis	834:839	arg1	TGA					926:928	TGA	926:928	TGA	926:928	The morphology, size, surface charge, composition and thermal stability of nanogel was characterized by UV-vis, Raman and FTIR spectroscopy, TEM, FESEM, EDX, XRD, DLS and zeta potential analysis, TGA and DTG.
32747289	5	69	theme	UV-vis	834:839	arg1	DTG					934:936	DTG	934:936	DTG	934:936	The morphology, size, surface charge, composition and thermal stability of nanogel was characterized by UV-vis, Raman and FTIR spectroscopy, TEM, FESEM, EDX, XRD, DLS and zeta potential analysis, TGA and DTG.
32747289	5	69	theme	UV-vis	834:839	arg1	spectroscopy					857:868	UV-vis, Raman and FTIR spectroscopy	834:868	spectroscopy	857:868	The morphology, size, surface charge, composition and thermal stability of nanogel was characterized by UV-vis, Raman and FTIR spectroscopy, TEM, FESEM, EDX, XRD, DLS and zeta potential analysis, TGA and DTG.
32747289	7	70	theme	<	1141:1141	arg1	precision					1130:1138	satisfactory precision	1117:1138	satisfactory precision (< 3.5 %)	1117:1148	Wide linearity from 5 to 1000 μM H2O2, with low limit of detection of 1.0 μM in addition to satisfactory precision (< 3.5 %) and recovery (95-105 %) and high selectivity was achieved.
32747289	7	70	theme	<	1141:1141	arg1	%					1147:1147	< 3.5 %	1141:1147	< 3.5 %	1141:1147	Wide linearity from 5 to 1000 μM H2O2, with low limit of detection of 1.0 μM in addition to satisfactory precision (< 3.5 %) and recovery (95-105 %) and high selectivity was achieved.
33815695	8	0	theme	host	1796:1799	arg1	cells					1812:1816	host epithelial cells	1796:1816	host epithelial cells	1796:1816	This study reveals the ability of DNA, RNA and peptidoglycan associated with MVs to activate PRRs in host epithelial cells, and their intracellular degradation via autophagy.
33815695	7	1	theme	puncta	1597:1602	arg1	accumulation					1566:1577	accumulation	1566:1577	accumulation of autophagosomal puncta that colocalized with MVs, revealing the ability of the host to degrade MVs via autophagy	1566:1692	Moreover, S. aureus MVs induced the formation of and colocalized with autophagosomes in epithelial cells, while inhibition of lysosomal acidification using bafilomycin A1 resulted in accumulation of autophagosomal puncta that colocalized with MVs, revealing the ability of the host to degrade MVs via autophagy.
33815695	2	2	theme	MVs	430:432	arg1	contents					386:393	contents	386:393	contents	386:393	Here we examine the morphology, contents and immunostimulatory functions of MVs produced by three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain.
33815695	2	2	theme	MVs	430:432	arg1	functions					417:425	immunostimulatory functions	399:425	immunostimulatory functions	399:425	Here we examine the morphology, contents and immunostimulatory functions of MVs produced by three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain.
33815695	2	2	theme	MVs	430:432	arg1	morphology					374:383	morphology	374:383	morphology	374:383	Here we examine the morphology, contents and immunostimulatory functions of MVs produced by three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain.
33815695	9	3	theme	immunostimulatory	1918:1934	arg1	roles					1936:1940	the immunostimulatory roles	1914:1940	the immunostimulatory roles of Gram-positive bacterial MVs	1914:1971	These findings advance our understanding of the immunostimulatory roles of Gram-positive bacterial MVs in mediating pathogenesis, and their intracellular fate within the host.
33815695	0	4	theme	innate	107:112	arg1	receptors					121:129	innate immune receptors	107:129	innate immune receptors	107:129	Staphylococcus aureus membrane vesicles contain immunostimulatory DNA, RNA and peptidoglycan that activate innate immune receptors and induce autophagy.
33815695	6	5	theme	chemokine	1211:1219	arg1	release					1221:1227	cytokine and chemokine release	1198:1227	release	1221:1227	S. aureus MVs activated Toll-like receptor (TLR) 2, 7, 8, 9 and nucleotide-binding oligomerization domain containing protein 2 (NOD2) signalling and promoted cytokine and chemokine release by epithelial cells, thus identifying that MV-associated MAMPs including DNA, RNA and peptidoglycan are detected by pattern recognition receptors (PRRs).
33815695	6	6	theme	protein	1157:1163	arg1	signalling					1174:1183	protein 2 (NOD2) signalling	1157:1183	protein 2 (NOD2) signalling	1157:1183	S. aureus MVs activated Toll-like receptor (TLR) 2, 7, 8, 9 and nucleotide-binding oligomerization domain containing protein 2 (NOD2) signalling and promoted cytokine and chemokine release by epithelial cells, thus identifying that MV-associated MAMPs including DNA, RNA and peptidoglycan are detected by pattern recognition receptors (PRRs).
33815695	7	7	theme	epithelial	1471:1480	arg1	cells					1482:1486	epithelial cells	1471:1486	epithelial cells	1471:1486	Moreover, S. aureus MVs induced the formation of and colocalized with autophagosomes in epithelial cells, while inhibition of lysosomal acidification using bafilomycin A1 resulted in accumulation of autophagosomal puncta that colocalized with MVs, revealing the ability of the host to degrade MVs via autophagy.
33815695	0	8	contain	contain	40:46	arg2	DNA					66:68	immunostimulatory DNA	48:68	immunostimulatory DNA	48:68	Staphylococcus aureus membrane vesicles contain immunostimulatory DNA, RNA and peptidoglycan that activate innate immune receptors and induce autophagy.
33815695	0	8	contain	contain	40:46	arg2	RNA					71:73	RNA	71:73	RNA	71:73	Staphylococcus aureus membrane vesicles contain immunostimulatory DNA, RNA and peptidoglycan that activate innate immune receptors and induce autophagy.
33815695	0	8	contain	contain	40:46	arg1	vesicles					31:38	Staphylococcus aureus membrane vesicles	0:38	Staphylococcus aureus membrane vesicles	0:38	Staphylococcus aureus membrane vesicles contain immunostimulatory DNA, RNA and peptidoglycan that activate innate immune receptors and induce autophagy.
33815695	0	8	contain	contain	40:46	arg2	peptidoglycan					79:91	peptidoglycan	79:91	peptidoglycan	79:91	Staphylococcus aureus membrane vesicles contain immunostimulatory DNA, RNA and peptidoglycan that activate innate immune receptors and induce autophagy.
33815695	2	9	dep	morphology	374:383	arg1	the					370:372	the	370:372	the	370:372	Here we examine the morphology, contents and immunostimulatory functions of MVs produced by three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain.
33815695	5	10	from	strain	1032:1037	arg1	MVs					1008:1010	MVs	1008:1010	MVs from each S. aureus strain	1008:1037	Furthermore, we detected variability in the amount and composition of protein, nucleic acid and peptidoglycan cargo carried by MVs from each S. aureus strain.
33815695	6	11	theme	S. aureus	1040:1048	arg1	MVs					1050:1052	S. aureus MVs	1040:1052	S. aureus MVs	1040:1052	S. aureus MVs activated Toll-like receptor (TLR) 2, 7, 8, 9 and nucleotide-binding oligomerization domain containing protein 2 (NOD2) signalling and promoted cytokine and chemokine release by epithelial cells, thus identifying that MV-associated MAMPs including DNA, RNA and peptidoglycan are detected by pattern recognition receptors (PRRs).
33815695	3	12	theme	nucleic	777:783	arg1	acids					785:789	nucleic acids	777:789	nucleic acids	777:789	We observed differences in the number and morphology of MVs produced by each strain and showed that they contain microbe-associated molecular patterns (MAMPs) including protein, nucleic acids and peptidoglycan.
33815695	2	13	dep	strains	474:480	arg1	isolate					558:564	a methicillin sensitive clinical isolate	525:564	a methicillin sensitive clinical isolate	525:564	Here we examine the morphology, contents and immunostimulatory functions of MVs produced by three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain.
33815695	2	13	dep	strains	474:480	arg1	strain					591:596	a laboratory-adapted strain	570:596	a laboratory-adapted strain	570:596	Here we examine the morphology, contents and immunostimulatory functions of MVs produced by three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain.
33815695	2	13	dep	strains	474:480	arg1	strains					474:480	three Staphylococcus aureus strains	446:480	three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain	446:596	Here we examine the morphology, contents and immunostimulatory functions of MVs produced by three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain.
33815695	2	13	dep	strains	474:480	arg1	isolate					516:522	a methicillin resistant clinical isolate	483:522	a methicillin resistant clinical isolate	483:522	Here we examine the morphology, contents and immunostimulatory functions of MVs produced by three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain.
33815695	7	14	theme	autophagosomal	1582:1595	arg1	puncta					1597:1602	autophagosomal puncta	1582:1602	autophagosomal puncta that colocalized with MVs, revealing the ability of the host to degrade MVs via autophagy	1582:1692	Moreover, S. aureus MVs induced the formation of and colocalized with autophagosomes in epithelial cells, while inhibition of lysosomal acidification using bafilomycin A1 resulted in accumulation of autophagosomal puncta that colocalized with MVs, revealing the ability of the host to degrade MVs via autophagy.
33815695	4	15	theme	RNA	833:835	arg1	Analysis					810:817	Analysis	810:817	Analysis of MV-derived RNA	810:835	Analysis of MV-derived RNA indicated the presence of small RNA (sRNA).
33815695	9	16	theme	roles	1936:1940	arg1	understanding					1897:1909	our understanding	1893:1909	our understanding of the immunostimulatory roles of Gram-positive bacterial MVs	1893:1971	These findings advance our understanding of the immunostimulatory roles of Gram-positive bacterial MVs in mediating pathogenesis, and their intracellular fate within the host.
33815695	3	17	contain	contain	704:710	arg2	patterns					741:748	microbe-associated molecular patterns	712:748	microbe-associated molecular patterns (MAMPs) including protein, nucleic acids and peptidoglycan	712:807	We observed differences in the number and morphology of MVs produced by each strain and showed that they contain microbe-associated molecular patterns (MAMPs) including protein, nucleic acids and peptidoglycan.
33815695	3	17	contain	contain	704:710	arg2	MAMPs					751:755	MAMPs	751:755	MAMPs	751:755	We observed differences in the number and morphology of MVs produced by each strain and showed that they contain microbe-associated molecular patterns (MAMPs) including protein, nucleic acids and peptidoglycan.
33815695	3	17	contain	contain	704:710	arg2	acids					785:789	nucleic acids	777:789	nucleic acids	777:789	We observed differences in the number and morphology of MVs produced by each strain and showed that they contain microbe-associated molecular patterns (MAMPs) including protein, nucleic acids and peptidoglycan.
33815695	3	17	contain	contain	704:710	arg2	protein					768:774	protein	768:774	protein	768:774	We observed differences in the number and morphology of MVs produced by each strain and showed that they contain microbe-associated molecular patterns (MAMPs) including protein, nucleic acids and peptidoglycan.
33815695	3	17	contain	contain	704:710	arg2	peptidoglycan					795:807	peptidoglycan	795:807	peptidoglycan	795:807	We observed differences in the number and morphology of MVs produced by each strain and showed that they contain microbe-associated molecular patterns (MAMPs) including protein, nucleic acids and peptidoglycan.
33815695	3	17	contain	contain	704:710	arg1	they					699:702	they	699:702	they	699:702	We observed differences in the number and morphology of MVs produced by each strain and showed that they contain microbe-associated molecular patterns (MAMPs) including protein, nucleic acids and peptidoglycan.
33815695	0	18	theme	immune	114:119	arg1	receptors					121:129	innate immune receptors	107:129	innate immune receptors	107:129	Staphylococcus aureus membrane vesicles contain immunostimulatory DNA, RNA and peptidoglycan that activate innate immune receptors and induce autophagy.
33815695	4	19	theme	MV-derived	822:831	arg1	RNA					833:835	MV-derived RNA	822:835	MV-derived RNA	822:835	Analysis of MV-derived RNA indicated the presence of small RNA (sRNA).
33815695	0	20	theme	aureus	15:20	arg1	vesicles					31:38	Staphylococcus aureus membrane vesicles	0:38	Staphylococcus aureus membrane vesicles	0:38	Staphylococcus aureus membrane vesicles contain immunostimulatory DNA, RNA and peptidoglycan that activate innate immune receptors and induce autophagy.
33815695	7	21	theme	host	1660:1663	arg1	ability					1645:1651	the ability	1641:1651	the ability of the host to degrade MVs via autophagy	1641:1692	Moreover, S. aureus MVs induced the formation of and colocalized with autophagosomes in epithelial cells, while inhibition of lysosomal acidification using bafilomycin A1 resulted in accumulation of autophagosomal puncta that colocalized with MVs, revealing the ability of the host to degrade MVs via autophagy.
33815695	8	22	theme	intracellular	1829:1841	arg1	degradation					1843:1853	their intracellular degradation	1823:1853	their intracellular degradation	1823:1853	This study reveals the ability of DNA, RNA and peptidoglycan associated with MVs to activate PRRs in host epithelial cells, and their intracellular degradation via autophagy.
33815695	6	23	theme	NOD2	1168:1171	arg1	signalling					1174:1183	protein 2 (NOD2) signalling	1157:1183	protein 2 (NOD2) signalling	1157:1183	S. aureus MVs activated Toll-like receptor (TLR) 2, 7, 8, 9 and nucleotide-binding oligomerization domain containing protein 2 (NOD2) signalling and promoted cytokine and chemokine release by epithelial cells, thus identifying that MV-associated MAMPs including DNA, RNA and peptidoglycan are detected by pattern recognition receptors (PRRs).
33815695	8	24	theme	DNA	1729:1731	arg1	ability					1718:1724	the ability	1714:1724	the ability of DNA, RNA and peptidoglycan associated with MVs to activate PRRs in host epithelial cells	1714:1816	This study reveals the ability of DNA, RNA and peptidoglycan associated with MVs to activate PRRs in host epithelial cells, and their intracellular degradation via autophagy.
33815695	0	25	theme	Staphylococcus	0:13	arg1	vesicles					31:38	Staphylococcus aureus membrane vesicles	0:38	Staphylococcus aureus membrane vesicles	0:38	Staphylococcus aureus membrane vesicles contain immunostimulatory DNA, RNA and peptidoglycan that activate innate immune receptors and induce autophagy.
33815695	7	26	theme	acidification	1519:1531	arg1	inhibition					1495:1504	inhibition	1495:1504	inhibition of lysosomal acidification using bafilomycin A1	1495:1552	Moreover, S. aureus MVs induced the formation of and colocalized with autophagosomes in epithelial cells, while inhibition of lysosomal acidification using bafilomycin A1 resulted in accumulation of autophagosomal puncta that colocalized with MVs, revealing the ability of the host to degrade MVs via autophagy.
33815695	6	27	theme	MV-associated	1272:1284	arg1	MAMPs					1286:1290	MV-associated MAMPs	1272:1290	MV-associated MAMPs including DNA, RNA and peptidoglycan	1272:1327	S. aureus MVs activated Toll-like receptor (TLR) 2, 7, 8, 9 and nucleotide-binding oligomerization domain containing protein 2 (NOD2) signalling and promoted cytokine and chemokine release by epithelial cells, thus identifying that MV-associated MAMPs including DNA, RNA and peptidoglycan are detected by pattern recognition receptors (PRRs).
33815695	9	28	theme	bacterial	1959:1967	arg1	MVs					1969:1971	Gram-positive bacterial MVs	1945:1971	Gram-positive bacterial MVs	1945:1971	These findings advance our understanding of the immunostimulatory roles of Gram-positive bacterial MVs in mediating pathogenesis, and their intracellular fate within the host.
33815695	2	29	theme	immunostimulatory	399:415	arg1	functions					417:425	immunostimulatory functions	399:425	immunostimulatory functions	399:425	Here we examine the morphology, contents and immunostimulatory functions of MVs produced by three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain.
33815695	6	30	theme	recognition	1353:1363	arg1	PRRs					1376:1379	PRRs	1376:1379	PRRs	1376:1379	S. aureus MVs activated Toll-like receptor (TLR) 2, 7, 8, 9 and nucleotide-binding oligomerization domain containing protein 2 (NOD2) signalling and promoted cytokine and chemokine release by epithelial cells, thus identifying that MV-associated MAMPs including DNA, RNA and peptidoglycan are detected by pattern recognition receptors (PRRs).
33815695	6	30	theme	recognition	1353:1363	arg1	receptors					1365:1373	pattern recognition receptors	1345:1373	pattern recognition receptors (PRRs)	1345:1380	S. aureus MVs activated Toll-like receptor (TLR) 2, 7, 8, 9 and nucleotide-binding oligomerization domain containing protein 2 (NOD2) signalling and promoted cytokine and chemokine release by epithelial cells, thus identifying that MV-associated MAMPs including DNA, RNA and peptidoglycan are detected by pattern recognition receptors (PRRs).
33815695	3	31	dep	number	630:635	arg1	the					626:628	the	626:628	the	626:628	We observed differences in the number and morphology of MVs produced by each strain and showed that they contain microbe-associated molecular patterns (MAMPs) including protein, nucleic acids and peptidoglycan.
33815695	8	32	theme	RNA	1734:1736	arg1	ability					1718:1724	the ability	1714:1724	the ability of DNA, RNA and peptidoglycan associated with MVs to activate PRRs in host epithelial cells	1714:1816	This study reveals the ability of DNA, RNA and peptidoglycan associated with MVs to activate PRRs in host epithelial cells, and their intracellular degradation via autophagy.
33815695	2	33	theme	Staphylococcus	452:465	arg1	isolate					516:522	a methicillin resistant clinical isolate	483:522	a methicillin resistant clinical isolate	483:522	Here we examine the morphology, contents and immunostimulatory functions of MVs produced by three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain.
33815695	2	33	theme	Staphylococcus	452:465	arg1	strains					474:480	three Staphylococcus aureus strains	446:480	three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain	446:596	Here we examine the morphology, contents and immunostimulatory functions of MVs produced by three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain.
33815695	2	33	theme	Staphylococcus	452:465	arg1	strain					591:596	a laboratory-adapted strain	570:596	a laboratory-adapted strain	570:596	Here we examine the morphology, contents and immunostimulatory functions of MVs produced by three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain.
33815695	2	34	theme	aureus	467:472	arg1	isolate					516:522	a methicillin resistant clinical isolate	483:522	a methicillin resistant clinical isolate	483:522	Here we examine the morphology, contents and immunostimulatory functions of MVs produced by three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain.
33815695	2	34	theme	aureus	467:472	arg1	strains					474:480	three Staphylococcus aureus strains	446:480	three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain	446:596	Here we examine the morphology, contents and immunostimulatory functions of MVs produced by three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain.
33815695	2	34	theme	aureus	467:472	arg1	strain					591:596	a laboratory-adapted strain	570:596	a laboratory-adapted strain	570:596	Here we examine the morphology, contents and immunostimulatory functions of MVs produced by three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain.
33815695	6	35	theme	pattern	1345:1351	arg1	PRRs					1376:1379	PRRs	1376:1379	PRRs	1376:1379	S. aureus MVs activated Toll-like receptor (TLR) 2, 7, 8, 9 and nucleotide-binding oligomerization domain containing protein 2 (NOD2) signalling and promoted cytokine and chemokine release by epithelial cells, thus identifying that MV-associated MAMPs including DNA, RNA and peptidoglycan are detected by pattern recognition receptors (PRRs).
33815695	6	35	theme	pattern	1345:1351	arg1	receptors					1365:1373	pattern recognition receptors	1345:1373	pattern recognition receptors (PRRs)	1345:1380	S. aureus MVs activated Toll-like receptor (TLR) 2, 7, 8, 9 and nucleotide-binding oligomerization domain containing protein 2 (NOD2) signalling and promoted cytokine and chemokine release by epithelial cells, thus identifying that MV-associated MAMPs including DNA, RNA and peptidoglycan are detected by pattern recognition receptors (PRRs).
33815695	5	36	dep	amount	925:930	arg1	the					921:923	the	921:923	the	921:923	Furthermore, we detected variability in the amount and composition of protein, nucleic acid and peptidoglycan cargo carried by MVs from each S. aureus strain.
33815695	0	37	theme	immunostimulatory	48:64	arg1	DNA					66:68	immunostimulatory DNA	48:68	immunostimulatory DNA	48:68	Staphylococcus aureus membrane vesicles contain immunostimulatory DNA, RNA and peptidoglycan that activate innate immune receptors and induce autophagy.
33815695	5	38	theme	protein	951:957	arg1	peptidoglycan					977:989	peptidoglycan	977:989	peptidoglycan	977:989	Furthermore, we detected variability in the amount and composition of protein, nucleic acid and peptidoglycan cargo carried by MVs from each S. aureus strain.
33815695	5	38	theme	protein	951:957	arg1	protein					951:957	protein	951:957	protein	951:957	Furthermore, we detected variability in the amount and composition of protein, nucleic acid and peptidoglycan cargo carried by MVs from each S. aureus strain.
33815695	5	38	theme	protein	951:957	arg1	acid					968:971	nucleic acid	960:971	nucleic acid	960:971	Furthermore, we detected variability in the amount and composition of protein, nucleic acid and peptidoglycan cargo carried by MVs from each S. aureus strain.
33815695	5	38	theme	protein	951:957	arg1	composition					936:946	composition	936:946	composition	936:946	Furthermore, we detected variability in the amount and composition of protein, nucleic acid and peptidoglycan cargo carried by MVs from each S. aureus strain.
33815695	5	38	theme	protein	951:957	arg1	amount					925:930	amount	925:930	amount	925:930	Furthermore, we detected variability in the amount and composition of protein, nucleic acid and peptidoglycan cargo carried by MVs from each S. aureus strain.
33815695	4	39	theme	RNA	869:871	arg1	presence					851:858	the presence	847:858	the presence of small RNA (sRNA)	847:878	Analysis of MV-derived RNA indicated the presence of small RNA (sRNA).
33815695	8	40	theme	peptidoglycan	1742:1754	arg1	ability					1718:1724	the ability	1714:1724	the ability of DNA, RNA and peptidoglycan associated with MVs to activate PRRs in host epithelial cells	1714:1816	This study reveals the ability of DNA, RNA and peptidoglycan associated with MVs to activate PRRs in host epithelial cells, and their intracellular degradation via autophagy.
33815695	1	41	theme	membrane	197:204	arg1	MVs					216:218	MVs	216:218	MVs	216:218	Gram-positive bacteria ubiquitously produce membrane vesicles (MVs), and although they contribute to biological functions, our knowledge regarding their composition and immunogenicity remains limited.
33815695	1	41	theme	membrane	197:204	arg1	vesicles					206:213	membrane vesicles	197:213	membrane vesicles (MVs)	197:219	Gram-positive bacteria ubiquitously produce membrane vesicles (MVs), and although they contribute to biological functions, our knowledge regarding their composition and immunogenicity remains limited.
33815695	2	42	theme	clinical	507:514	arg1	isolate					558:564	a methicillin sensitive clinical isolate	525:564	a methicillin sensitive clinical isolate	525:564	Here we examine the morphology, contents and immunostimulatory functions of MVs produced by three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain.
33815695	2	42	theme	clinical	507:514	arg1	strains					474:480	three Staphylococcus aureus strains	446:480	three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain	446:596	Here we examine the morphology, contents and immunostimulatory functions of MVs produced by three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain.
33815695	2	42	theme	clinical	507:514	arg1	isolate					516:522	a methicillin resistant clinical isolate	483:522	a methicillin resistant clinical isolate	483:522	Here we examine the morphology, contents and immunostimulatory functions of MVs produced by three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain.
33815695	4	43	theme	small	863:867	arg1	RNA					869:871	small RNA	863:871	small RNA (sRNA)	863:878	Analysis of MV-derived RNA indicated the presence of small RNA (sRNA).
33815695	4	43	theme	small	863:867	arg1	sRNA					874:877	sRNA	874:877	sRNA	874:877	Analysis of MV-derived RNA indicated the presence of small RNA (sRNA).
33815695	2	44	theme	clinical	549:556	arg1	isolate					558:564	a methicillin sensitive clinical isolate	525:564	a methicillin sensitive clinical isolate	525:564	Here we examine the morphology, contents and immunostimulatory functions of MVs produced by three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain.
33815695	2	44	theme	clinical	549:556	arg1	isolate					516:522	a methicillin resistant clinical isolate	483:522	a methicillin resistant clinical isolate	483:522	Here we examine the morphology, contents and immunostimulatory functions of MVs produced by three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain.
33815695	2	45	theme	resistant	497:505	arg1	isolate					558:564	a methicillin sensitive clinical isolate	525:564	a methicillin sensitive clinical isolate	525:564	Here we examine the morphology, contents and immunostimulatory functions of MVs produced by three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain.
33815695	2	45	theme	resistant	497:505	arg1	strains					474:480	three Staphylococcus aureus strains	446:480	three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain	446:596	Here we examine the morphology, contents and immunostimulatory functions of MVs produced by three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain.
33815695	2	45	theme	resistant	497:505	arg1	isolate					516:522	a methicillin resistant clinical isolate	483:522	a methicillin resistant clinical isolate	483:522	Here we examine the morphology, contents and immunostimulatory functions of MVs produced by three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain.
33815695	6	46	contain	containing	1146:1155	arg2	signalling					1174:1183	protein 2 (NOD2) signalling	1157:1183	protein 2 (NOD2) signalling	1157:1183	S. aureus MVs activated Toll-like receptor (TLR) 2, 7, 8, 9 and nucleotide-binding oligomerization domain containing protein 2 (NOD2) signalling and promoted cytokine and chemokine release by epithelial cells, thus identifying that MV-associated MAMPs including DNA, RNA and peptidoglycan are detected by pattern recognition receptors (PRRs).
33815695	6	46	contain	containing	1146:1155	arg1	domain					1139:1144	nucleotide-binding oligomerization domain	1104:1144	nucleotide-binding oligomerization domain containing protein 2 (NOD2) signalling	1104:1183	S. aureus MVs activated Toll-like receptor (TLR) 2, 7, 8, 9 and nucleotide-binding oligomerization domain containing protein 2 (NOD2) signalling and promoted cytokine and chemokine release by epithelial cells, thus identifying that MV-associated MAMPs including DNA, RNA and peptidoglycan are detected by pattern recognition receptors (PRRs).
33815695	6	47	theme	epithelial	1232:1241	arg1	cells					1243:1247	epithelial cells	1232:1247	epithelial cells	1232:1247	S. aureus MVs activated Toll-like receptor (TLR) 2, 7, 8, 9 and nucleotide-binding oligomerization domain containing protein 2 (NOD2) signalling and promoted cytokine and chemokine release by epithelial cells, thus identifying that MV-associated MAMPs including DNA, RNA and peptidoglycan are detected by pattern recognition receptors (PRRs).
33815695	9	48	theme	MVs	1969:1971	arg1	roles					1936:1940	the immunostimulatory roles	1914:1940	the immunostimulatory roles of Gram-positive bacterial MVs	1914:1971	These findings advance our understanding of the immunostimulatory roles of Gram-positive bacterial MVs in mediating pathogenesis, and their intracellular fate within the host.
33815695	2	49	theme	laboratory-adapted	572:589	arg1	strain					591:596	a laboratory-adapted strain	570:596	a laboratory-adapted strain	570:596	Here we examine the morphology, contents and immunostimulatory functions of MVs produced by three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain.
33815695	2	49	theme	laboratory-adapted	572:589	arg1	strains					474:480	three Staphylococcus aureus strains	446:480	three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain	446:596	Here we examine the morphology, contents and immunostimulatory functions of MVs produced by three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain.
33815695	3	50	theme	microbe-associated	712:729	arg1	MAMPs					751:755	MAMPs	751:755	MAMPs	751:755	We observed differences in the number and morphology of MVs produced by each strain and showed that they contain microbe-associated molecular patterns (MAMPs) including protein, nucleic acids and peptidoglycan.
33815695	3	50	theme	microbe-associated	712:729	arg1	peptidoglycan					795:807	peptidoglycan	795:807	peptidoglycan	795:807	We observed differences in the number and morphology of MVs produced by each strain and showed that they contain microbe-associated molecular patterns (MAMPs) including protein, nucleic acids and peptidoglycan.
33815695	3	50	theme	microbe-associated	712:729	arg1	patterns					741:748	microbe-associated molecular patterns	712:748	microbe-associated molecular patterns (MAMPs) including protein, nucleic acids and peptidoglycan	712:807	We observed differences in the number and morphology of MVs produced by each strain and showed that they contain microbe-associated molecular patterns (MAMPs) including protein, nucleic acids and peptidoglycan.
33815695	3	50	theme	microbe-associated	712:729	arg1	acids					785:789	nucleic acids	777:789	nucleic acids	777:789	We observed differences in the number and morphology of MVs produced by each strain and showed that they contain microbe-associated molecular patterns (MAMPs) including protein, nucleic acids and peptidoglycan.
33815695	3	50	theme	microbe-associated	712:729	arg1	protein					768:774	protein	768:774	protein	768:774	We observed differences in the number and morphology of MVs produced by each strain and showed that they contain microbe-associated molecular patterns (MAMPs) including protein, nucleic acids and peptidoglycan.
33815695	7	51	theme	bafilomycin	1539:1549	arg1	A1					1551:1552	bafilomycin A1	1539:1552	bafilomycin A1	1539:1552	Moreover, S. aureus MVs induced the formation of and colocalized with autophagosomes in epithelial cells, while inhibition of lysosomal acidification using bafilomycin A1 resulted in accumulation of autophagosomal puncta that colocalized with MVs, revealing the ability of the host to degrade MVs via autophagy.
33815695	4	52	link	MV-derived	822:831	arg1	RNA					833:835	MV-derived RNA	822:835	MV-derived RNA	822:835	Analysis of MV-derived RNA indicated the presence of small RNA (sRNA).
33815695	6	53	theme	cytokine	1198:1205	arg1	release					1221:1227	cytokine and chemokine release	1198:1227	release	1221:1227	S. aureus MVs activated Toll-like receptor (TLR) 2, 7, 8, 9 and nucleotide-binding oligomerization domain containing protein 2 (NOD2) signalling and promoted cytokine and chemokine release by epithelial cells, thus identifying that MV-associated MAMPs including DNA, RNA and peptidoglycan are detected by pattern recognition receptors (PRRs).
33815695	3	54	theme	molecular	731:739	arg1	MAMPs					751:755	MAMPs	751:755	MAMPs	751:755	We observed differences in the number and morphology of MVs produced by each strain and showed that they contain microbe-associated molecular patterns (MAMPs) including protein, nucleic acids and peptidoglycan.
33815695	3	54	theme	molecular	731:739	arg1	peptidoglycan					795:807	peptidoglycan	795:807	peptidoglycan	795:807	We observed differences in the number and morphology of MVs produced by each strain and showed that they contain microbe-associated molecular patterns (MAMPs) including protein, nucleic acids and peptidoglycan.
33815695	3	54	theme	molecular	731:739	arg1	patterns					741:748	microbe-associated molecular patterns	712:748	microbe-associated molecular patterns (MAMPs) including protein, nucleic acids and peptidoglycan	712:807	We observed differences in the number and morphology of MVs produced by each strain and showed that they contain microbe-associated molecular patterns (MAMPs) including protein, nucleic acids and peptidoglycan.
33815695	3	54	theme	molecular	731:739	arg1	acids					785:789	nucleic acids	777:789	nucleic acids	777:789	We observed differences in the number and morphology of MVs produced by each strain and showed that they contain microbe-associated molecular patterns (MAMPs) including protein, nucleic acids and peptidoglycan.
33815695	3	54	theme	molecular	731:739	arg1	protein					768:774	protein	768:774	protein	768:774	We observed differences in the number and morphology of MVs produced by each strain and showed that they contain microbe-associated molecular patterns (MAMPs) including protein, nucleic acids and peptidoglycan.
33815695	7	55	theme	S. aureus	1393:1401	arg1	MVs					1403:1405	S. aureus MVs	1393:1405	S. aureus MVs	1393:1405	Moreover, S. aureus MVs induced the formation of and colocalized with autophagosomes in epithelial cells, while inhibition of lysosomal acidification using bafilomycin A1 resulted in accumulation of autophagosomal puncta that colocalized with MVs, revealing the ability of the host to degrade MVs via autophagy.
33815695	6	56	theme	oligomerization	1123:1137	arg1	domain					1139:1144	nucleotide-binding oligomerization domain	1104:1144	nucleotide-binding oligomerization domain containing protein 2 (NOD2) signalling	1104:1183	S. aureus MVs activated Toll-like receptor (TLR) 2, 7, 8, 9 and nucleotide-binding oligomerization domain containing protein 2 (NOD2) signalling and promoted cytokine and chemokine release by epithelial cells, thus identifying that MV-associated MAMPs including DNA, RNA and peptidoglycan are detected by pattern recognition receptors (PRRs).
33815695	3	57	from	differences	611:621	arg1	morphology					641:650	morphology	641:650	morphology	641:650	We observed differences in the number and morphology of MVs produced by each strain and showed that they contain microbe-associated molecular patterns (MAMPs) including protein, nucleic acids and peptidoglycan.
33815695	3	57	from	differences	611:621	arg1	number					630:635	number	630:635	number	630:635	We observed differences in the number and morphology of MVs produced by each strain and showed that they contain microbe-associated molecular patterns (MAMPs) including protein, nucleic acids and peptidoglycan.
33815695	6	58	theme	Toll-like	1064:1072	arg1	TLR					1084:1086	Toll-like receptor (TLR) 2	1064:1089	Toll-like receptor (TLR) 2	1064:1089	S. aureus MVs activated Toll-like receptor (TLR) 2, 7, 8, 9 and nucleotide-binding oligomerization domain containing protein 2 (NOD2) signalling and promoted cytokine and chemokine release by epithelial cells, thus identifying that MV-associated MAMPs including DNA, RNA and peptidoglycan are detected by pattern recognition receptors (PRRs).
33815695	6	59	theme	nucleotide-binding	1104:1121	arg1	domain					1139:1144	nucleotide-binding oligomerization domain	1104:1144	nucleotide-binding oligomerization domain containing protein 2 (NOD2) signalling	1104:1183	S. aureus MVs activated Toll-like receptor (TLR) 2, 7, 8, 9 and nucleotide-binding oligomerization domain containing protein 2 (NOD2) signalling and promoted cytokine and chemokine release by epithelial cells, thus identifying that MV-associated MAMPs including DNA, RNA and peptidoglycan are detected by pattern recognition receptors (PRRs).
33815695	7	60	theme	lysosomal	1509:1517	arg1	acidification					1519:1531	lysosomal acidification	1509:1531	lysosomal acidification using bafilomycin A1	1509:1552	Moreover, S. aureus MVs induced the formation of and colocalized with autophagosomes in epithelial cells, while inhibition of lysosomal acidification using bafilomycin A1 resulted in accumulation of autophagosomal puncta that colocalized with MVs, revealing the ability of the host to degrade MVs via autophagy.
33815695	5	61	theme	S. aureus	1022:1030	arg1	strain					1032:1037	each S. aureus strain	1017:1037	each S. aureus strain	1017:1037	Furthermore, we detected variability in the amount and composition of protein, nucleic acid and peptidoglycan cargo carried by MVs from each S. aureus strain.
33815695	9	62	theme	intracellular	2010:2022	arg1	fate					2024:2027	their intracellular fate	2004:2027	their intracellular fate within the host	2004:2043	These findings advance our understanding of the immunostimulatory roles of Gram-positive bacterial MVs in mediating pathogenesis, and their intracellular fate within the host.
33815695	5	63	theme	nucleic	960:966	arg1	protein					951:957	protein	951:957	protein	951:957	Furthermore, we detected variability in the amount and composition of protein, nucleic acid and peptidoglycan cargo carried by MVs from each S. aureus strain.
33815695	5	63	theme	nucleic	960:966	arg1	acid					968:971	nucleic acid	960:971	nucleic acid	960:971	Furthermore, we detected variability in the amount and composition of protein, nucleic acid and peptidoglycan cargo carried by MVs from each S. aureus strain.
33815695	5	63	theme	nucleic	960:966	arg1	amount					925:930	amount	925:930	amount	925:930	Furthermore, we detected variability in the amount and composition of protein, nucleic acid and peptidoglycan cargo carried by MVs from each S. aureus strain.
33815695	2	64	theme	sensitive	539:547	arg1	isolate					558:564	a methicillin sensitive clinical isolate	525:564	a methicillin sensitive clinical isolate	525:564	Here we examine the morphology, contents and immunostimulatory functions of MVs produced by three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain.
33815695	2	64	theme	sensitive	539:547	arg1	isolate					516:522	a methicillin resistant clinical isolate	483:522	a methicillin resistant clinical isolate	483:522	Here we examine the morphology, contents and immunostimulatory functions of MVs produced by three Staphylococcus aureus strains; a methicillin resistant clinical isolate, a methicillin sensitive clinical isolate and a laboratory-adapted strain.
33815695	6	65	theme	receptor	1074:1081	arg1	TLR					1084:1086	Toll-like receptor (TLR) 2	1064:1089	Toll-like receptor (TLR) 2	1064:1089	S. aureus MVs activated Toll-like receptor (TLR) 2, 7, 8, 9 and nucleotide-binding oligomerization domain containing protein 2 (NOD2) signalling and promoted cytokine and chemokine release by epithelial cells, thus identifying that MV-associated MAMPs including DNA, RNA and peptidoglycan are detected by pattern recognition receptors (PRRs).
33815695	3	66	theme	MVs	655:657	arg1	morphology					641:650	morphology	641:650	morphology	641:650	We observed differences in the number and morphology of MVs produced by each strain and showed that they contain microbe-associated molecular patterns (MAMPs) including protein, nucleic acids and peptidoglycan.
33815695	3	66	theme	MVs	655:657	arg1	number					630:635	number	630:635	number	630:635	We observed differences in the number and morphology of MVs produced by each strain and showed that they contain microbe-associated molecular patterns (MAMPs) including protein, nucleic acids and peptidoglycan.
33815695	1	67	theme	Gram-positive	153:165	arg1	bacteria					167:174	Gram-positive bacteria	153:174	Gram-positive bacteria	153:174	Gram-positive bacteria ubiquitously produce membrane vesicles (MVs), and although they contribute to biological functions, our knowledge regarding their composition and immunogenicity remains limited.
33815695	9	68	theme	Gram-positive	1945:1957	arg1	MVs					1969:1971	Gram-positive bacterial MVs	1945:1971	Gram-positive bacterial MVs	1945:1971	These findings advance our understanding of the immunostimulatory roles of Gram-positive bacterial MVs in mediating pathogenesis, and their intracellular fate within the host.
33815695	8	69	from	PRRs	1788:1791	arg1	cells					1812:1816	host epithelial cells	1796:1816	host epithelial cells	1796:1816	This study reveals the ability of DNA, RNA and peptidoglycan associated with MVs to activate PRRs in host epithelial cells, and their intracellular degradation via autophagy.
33815695	0	70	theme	membrane	22:29	arg1	vesicles					31:38	Staphylococcus aureus membrane vesicles	0:38	Staphylococcus aureus membrane vesicles	0:38	Staphylococcus aureus membrane vesicles contain immunostimulatory DNA, RNA and peptidoglycan that activate innate immune receptors and induce autophagy.
33815695	8	71	theme	epithelial	1801:1810	arg1	cells					1812:1816	host epithelial cells	1796:1816	host epithelial cells	1796:1816	This study reveals the ability of DNA, RNA and peptidoglycan associated with MVs to activate PRRs in host epithelial cells, and their intracellular degradation via autophagy.
33815695	7	72	from	autophagosomes	1453:1466	arg1	cells					1482:1486	epithelial cells	1471:1486	epithelial cells	1471:1486	Moreover, S. aureus MVs induced the formation of and colocalized with autophagosomes in epithelial cells, while inhibition of lysosomal acidification using bafilomycin A1 resulted in accumulation of autophagosomal puncta that colocalized with MVs, revealing the ability of the host to degrade MVs via autophagy.
33815695	1	73	theme	biological	254:263	arg1	functions					265:273	biological functions	254:273	biological functions	254:273	Gram-positive bacteria ubiquitously produce membrane vesicles (MVs), and although they contribute to biological functions, our knowledge regarding their composition and immunogenicity remains limited.
32661667	12	0	theme	A4α	1053:1055	arg1	acid					1114:1117	the diagnostic diamino acid	1091:1117	the diagnostic diamino acid	1091:1117	The cell wall peptidoglycan contained A4α peptidoglycan type with lysine as the diagnostic diamino acid.
32661667	12	0	theme	A4α	1053:1055	arg1	type					1071:1074	A4α peptidoglycan type	1053:1074	A4α peptidoglycan type	1053:1074	The cell wall peptidoglycan contained A4α peptidoglycan type with lysine as the diagnostic diamino acid.
32661667	2	1	theme	positive	102:109	arg1	strain					143:148	A Gram-stain positive, motile, aerobic and rod-shaped strain	89:148	A Gram-stain positive, motile, aerobic and rod-shaped strain (MIC A30T)	89:159	A Gram-stain positive, motile, aerobic and rod-shaped strain (MIC A30T) was isolated from river sediment in Yuantouzhu park, Wuxi City, China.
32661667	2	1	theme	positive	102:109	arg1	A30T					155:158	MIC A30T	151:158	MIC A30T	151:158	A Gram-stain positive, motile, aerobic and rod-shaped strain (MIC A30T) was isolated from river sediment in Yuantouzhu park, Wuxi City, China.
32661667	5	2	theme	MIC	528:530	arg1	A30T					532:535	strain MIC A30T	521:535	strain MIC A30T	521:535	The DNA-DNA relatedness values between strain MIC A30T and these reference strains were below 30%.
32661667	4	3	theme	Arthrobacter	380:391	arg1	pokkaliiT					393:401	Arthrobacter pokkaliiT	380:401	Arthrobacter pokkaliiT (97.9%)	380:409	Strain MIC A30T was moderately related to Arthrobacter liuii CGMCC 1.12778T (97.9%), Arthrobacter pokkaliiT (97.9%) and Arthrobacter globiformis NBRC 12137T (96.7%) by 16S rRNA analysis.
32661667	4	3	theme	Arthrobacter	380:391	arg1	%					408:408	97.9%	404:408	97.9%	404:408	Strain MIC A30T was moderately related to Arthrobacter liuii CGMCC 1.12778T (97.9%), Arthrobacter pokkaliiT (97.9%) and Arthrobacter globiformis NBRC 12137T (96.7%) by 16S rRNA analysis.
32661667	13	4	theme	Arthrobacter	1254:1265	arg1	name					1234:1237	name	1234:1237	name	1234:1237	Based on several taxonomic results, strain MIC A30T is identified as a novel species in genus Arthrobacter, whose name is proposed as Arthrobacter sedimenti sp.
32661667	13	4	theme	Arthrobacter	1254:1265	arg1	sp					1277:1278	Arthrobacter sedimenti sp	1254:1278	Arthrobacter sedimenti sp	1254:1278	Based on several taxonomic results, strain MIC A30T is identified as a novel species in genus Arthrobacter, whose name is proposed as Arthrobacter sedimenti sp.
32661667	7	5	theme	CGMCC	730:734	arg1	1.12778T					736:743	A. liuii CGMCC 1.12778T	721:743	A. liuii CGMCC 1.12778T	721:743	Average nucleotide identity (ANI) and genome-to-genome distance (GGD) values between strain MIC A30T and A. liuii CGMCC 1.12778T were 60.34% and 29.39%, respectively.
32661667	13	6	theme	strain	1156:1161	arg1	A30T					1167:1170	strain MIC A30T	1156:1170	strain MIC A30T	1156:1170	Based on several taxonomic results, strain MIC A30T is identified as a novel species in genus Arthrobacter, whose name is proposed as Arthrobacter sedimenti sp.
32661667	13	6	theme	strain	1156:1161	arg1	species					1197:1203	a novel species	1189:1203	a novel species	1189:1203	Based on several taxonomic results, strain MIC A30T is identified as a novel species in genus Arthrobacter, whose name is proposed as Arthrobacter sedimenti sp.
32661667	2	7	theme	Gram-stain	91:100	arg1	strain					143:148	A Gram-stain positive, motile, aerobic and rod-shaped strain	89:148	A Gram-stain positive, motile, aerobic and rod-shaped strain (MIC A30T)	89:159	A Gram-stain positive, motile, aerobic and rod-shaped strain (MIC A30T) was isolated from river sediment in Yuantouzhu park, Wuxi City, China.
32661667	2	7	theme	Gram-stain	91:100	arg1	A30T					155:158	MIC A30T	151:158	MIC A30T	151:158	A Gram-stain positive, motile, aerobic and rod-shaped strain (MIC A30T) was isolated from river sediment in Yuantouzhu park, Wuxi City, China.
32661667	15	8	theme	19599T = CGMCC	1322:1335	arg1	A30T					1309:1312	MIC A30T	1305:1312	MIC A30T (= KACC 19599T = CGMCC 1.13474T)	1305:1345	The type strain is MIC A30T (= KACC 19599T = CGMCC 1.13474T).
32661667	15	8	theme	19599T = CGMCC	1322:1335	arg1	1.13474T					1337:1344	= KACC 19599T = CGMCC 1.13474T	1315:1344	= KACC 19599T = CGMCC 1.13474T	1315:1344	The type strain is MIC A30T (= KACC 19599T = CGMCC 1.13474T).
32661667	13	9	theme	sedimenti	1267:1275	arg1	name					1234:1237	name	1234:1237	name	1234:1237	Based on several taxonomic results, strain MIC A30T is identified as a novel species in genus Arthrobacter, whose name is proposed as Arthrobacter sedimenti sp.
32661667	13	9	theme	sedimenti	1267:1275	arg1	sp					1277:1278	Arthrobacter sedimenti sp	1254:1278	Arthrobacter sedimenti sp	1254:1278	Based on several taxonomic results, strain MIC A30T is identified as a novel species in genus Arthrobacter, whose name is proposed as Arthrobacter sedimenti sp.
32661667	13	10	theme	MIC	1163:1165	arg1	A30T					1167:1170	strain MIC A30T	1156:1170	strain MIC A30T	1156:1170	Based on several taxonomic results, strain MIC A30T is identified as a novel species in genus Arthrobacter, whose name is proposed as Arthrobacter sedimenti sp.
32661667	13	10	theme	MIC	1163:1165	arg1	species					1197:1203	a novel species	1189:1203	a novel species	1189:1203	Based on several taxonomic results, strain MIC A30T is identified as a novel species in genus Arthrobacter, whose name is proposed as Arthrobacter sedimenti sp.
32661667	3	11	theme	0-5.0	282:286	arg1	%					287:287	%	287:287	%	287:287	Growth occurred at 20-40 °C, at pH 6.0-9.0 and at 0-5.0% NaCl.
32661667	10	12	theme	fatty	898:902	arg1	acids					904:908	Major fatty acids	892:908	Major fatty acids	892:908	Major fatty acids were iso-C15:0, anteiso-C15:0 and anteiso-C17:0.
32661667	13	13	theme	several	1129:1135	arg1	results					1147:1153	several taxonomic results	1129:1153	several taxonomic results	1129:1153	Based on several taxonomic results, strain MIC A30T is identified as a novel species in genus Arthrobacter, whose name is proposed as Arthrobacter sedimenti sp.
32661667	1	14	theme	river	47:51	arg1	sediment					53:60	river sediment	47:60	river sediment in Yuantouzhu park, China	47:86	nov., isolated from river sediment in Yuantouzhu park, China.
32661667	1	15	from	sediment	53:60	arg1	China					82:86	China	82:86	China	82:86	nov., isolated from river sediment in Yuantouzhu park, China.
32661667	4	16	theme	rRNA	467:470	arg1	analysis					472:479	16S rRNA analysis	463:479	16S rRNA analysis	463:479	Strain MIC A30T was moderately related to Arthrobacter liuii CGMCC 1.12778T (97.9%), Arthrobacter pokkaliiT (97.9%) and Arthrobacter globiformis NBRC 12137T (96.7%) by 16S rRNA analysis.
32661667	3	17	theme	%	287:287	arg1	NaCl					289:292	0-5.0% NaCl	282:292	0-5.0% NaCl	282:292	Growth occurred at 20-40 °C, at pH 6.0-9.0 and at 0-5.0% NaCl.
32661667	7	18	theme	MIC	708:710	arg1	A30T					712:715	strain MIC A30T	701:715	strain MIC A30T	701:715	Average nucleotide identity (ANI) and genome-to-genome distance (GGD) values between strain MIC A30T and A. liuii CGMCC 1.12778T were 60.34% and 29.39%, respectively.
32661667	4	19	theme	16S	463:465	arg1	rRNA					467:470	16S rRNA	463:470	16S rRNA analysis	463:479	Strain MIC A30T was moderately related to Arthrobacter liuii CGMCC 1.12778T (97.9%), Arthrobacter pokkaliiT (97.9%) and Arthrobacter globiformis NBRC 12137T (96.7%) by 16S rRNA analysis.
32661667	12	20	theme	cell	1019:1022	arg1	peptidoglycan					1029:1041	The cell wall peptidoglycan	1015:1041	The cell wall peptidoglycan	1015:1041	The cell wall peptidoglycan contained A4α peptidoglycan type with lysine as the diagnostic diamino acid.
32661667	2	21	theme	aerobic	120:126	arg1	strain					143:148	A Gram-stain positive, motile, aerobic and rod-shaped strain	89:148	A Gram-stain positive, motile, aerobic and rod-shaped strain (MIC A30T)	89:159	A Gram-stain positive, motile, aerobic and rod-shaped strain (MIC A30T) was isolated from river sediment in Yuantouzhu park, Wuxi City, China.
32661667	2	21	theme	aerobic	120:126	arg1	A30T					155:158	MIC A30T	151:158	MIC A30T	151:158	A Gram-stain positive, motile, aerobic and rod-shaped strain (MIC A30T) was isolated from river sediment in Yuantouzhu park, Wuxi City, China.
32661667	5	22	theme	relatedness	494:504	arg1	values					506:511	The DNA-DNA relatedness values	482:511	The DNA-DNA relatedness values between strain MIC A30T and these reference strains	482:563	The DNA-DNA relatedness values between strain MIC A30T and these reference strains were below 30%.
32661667	5	23	theme	DNA-DNA	486:492	arg1	values					506:511	The DNA-DNA relatedness values	482:511	The DNA-DNA relatedness values between strain MIC A30T and these reference strains	482:563	The DNA-DNA relatedness values between strain MIC A30T and these reference strains were below 30%.
32661667	7	24	theme	strain	701:706	arg1	A30T					712:715	strain MIC A30T	701:715	strain MIC A30T	701:715	Average nucleotide identity (ANI) and genome-to-genome distance (GGD) values between strain MIC A30T and A. liuii CGMCC 1.12778T were 60.34% and 29.39%, respectively.
32661667	4	25	dep	1.12778T	362:369	arg1	12137T					445:450	NBRC 12137T	440:450	NBRC 12137T	440:450	Strain MIC A30T was moderately related to Arthrobacter liuii CGMCC 1.12778T (97.9%), Arthrobacter pokkaliiT (97.9%) and Arthrobacter globiformis NBRC 12137T (96.7%) by 16S rRNA analysis.
32661667	7	26	theme	nucleotide	624:633	arg1	ANI					645:647	ANI	645:647	ANI	645:647	Average nucleotide identity (ANI) and genome-to-genome distance (GGD) values between strain MIC A30T and A. liuii CGMCC 1.12778T were 60.34% and 29.39%, respectively.
32661667	7	26	theme	nucleotide	624:633	arg1	identity					635:642	Average nucleotide identity	616:642	Average nucleotide identity (ANI)	616:648	Average nucleotide identity (ANI) and genome-to-genome distance (GGD) values between strain MIC A30T and A. liuii CGMCC 1.12778T were 60.34% and 29.39%, respectively.
32661667	15	27	theme	= KACC	1315:1320	arg1	A30T					1309:1312	MIC A30T	1305:1312	MIC A30T (= KACC 19599T = CGMCC 1.13474T)	1305:1345	The type strain is MIC A30T (= KACC 19599T = CGMCC 1.13474T).
32661667	15	27	theme	= KACC	1315:1320	arg1	1.13474T					1337:1344	= KACC 19599T = CGMCC 1.13474T	1315:1344	= KACC 19599T = CGMCC 1.13474T	1315:1344	The type strain is MIC A30T (= KACC 19599T = CGMCC 1.13474T).
32661667	2	28	attach	isolated	165:172	arg1	sediment					185:192	river sediment	179:192	river sediment in Yuantouzhu park, Wuxi City, China	179:229	A Gram-stain positive, motile, aerobic and rod-shaped strain (MIC A30T) was isolated from river sediment in Yuantouzhu park, Wuxi City, China.
32661667	2	28	attach	isolated	165:172	arg2	A30T					155:158	MIC A30T	151:158	MIC A30T	151:158	A Gram-stain positive, motile, aerobic and rod-shaped strain (MIC A30T) was isolated from river sediment in Yuantouzhu park, Wuxi City, China.
32661667	2	28	attach	isolated	165:172	arg2	strain					143:148	A Gram-stain positive, motile, aerobic and rod-shaped strain	89:148	A Gram-stain positive, motile, aerobic and rod-shaped strain (MIC A30T)	89:159	A Gram-stain positive, motile, aerobic and rod-shaped strain (MIC A30T) was isolated from river sediment in Yuantouzhu park, Wuxi City, China.
32661667	0	29	theme	sedimenti	13:21	arg1	sp					23:24	Arthrobacter sedimenti sp	0:24	Arthrobacter sedimenti sp.	0:25	Arthrobacter sedimenti sp.
32661667	2	30	theme	motile	112:117	arg1	strain					143:148	A Gram-stain positive, motile, aerobic and rod-shaped strain	89:148	A Gram-stain positive, motile, aerobic and rod-shaped strain (MIC A30T)	89:159	A Gram-stain positive, motile, aerobic and rod-shaped strain (MIC A30T) was isolated from river sediment in Yuantouzhu park, Wuxi City, China.
32661667	2	30	theme	motile	112:117	arg1	A30T					155:158	MIC A30T	151:158	MIC A30T	151:158	A Gram-stain positive, motile, aerobic and rod-shaped strain (MIC A30T) was isolated from river sediment in Yuantouzhu park, Wuxi City, China.
32661667	15	31	theme	type	1290:1293	arg1	strain					1295:1300	The type strain	1286:1300	The type strain	1286:1300	The type strain is MIC A30T (= KACC 19599T = CGMCC 1.13474T).
32661667	15	31	theme	type	1290:1293	arg1	A30T					1309:1312	MIC A30T	1305:1312	MIC A30T (= KACC 19599T = CGMCC 1.13474T)	1305:1345	The type strain is MIC A30T (= KACC 19599T = CGMCC 1.13474T).
32661667	7	32	dep	A.	721:722	arg1	liuii					724:728	liuii	724:728	liuii	724:728	Average nucleotide identity (ANI) and genome-to-genome distance (GGD) values between strain MIC A30T and A. liuii CGMCC 1.12778T were 60.34% and 29.39%, respectively.
32661667	12	33	contain	contained	1043:1051	arg1	peptidoglycan					1029:1041	The cell wall peptidoglycan	1015:1041	The cell wall peptidoglycan	1015:1041	The cell wall peptidoglycan contained A4α peptidoglycan type with lysine as the diagnostic diamino acid.
32661667	12	33	contain	contained	1043:1051	arg2	type					1071:1074	A4α peptidoglycan type	1053:1074	A4α peptidoglycan type	1053:1074	The cell wall peptidoglycan contained A4α peptidoglycan type with lysine as the diagnostic diamino acid.
32661667	12	33	contain	contained	1043:1051	arg2	acid					1114:1117	the diagnostic diamino acid	1091:1117	the diagnostic diamino acid	1091:1117	The cell wall peptidoglycan contained A4α peptidoglycan type with lysine as the diagnostic diamino acid.
32661667	2	34	from	sediment	185:192	arg1	China					225:229	China	225:229	China	225:229	A Gram-stain positive, motile, aerobic and rod-shaped strain (MIC A30T) was isolated from river sediment in Yuantouzhu park, Wuxi City, China.
32661667	2	34	from	sediment	185:192	arg1	Wuxi					214:217	Wuxi	214:217	Wuxi	214:217	A Gram-stain positive, motile, aerobic and rod-shaped strain (MIC A30T) was isolated from river sediment in Yuantouzhu park, Wuxi City, China.
32661667	0	35	theme	Arthrobacter	0:11	arg1	sp					23:24	Arthrobacter sedimenti sp	0:24	Arthrobacter sedimenti sp.	0:25	Arthrobacter sedimenti sp.
32661667	7	36	theme	genome-to-genome	654:669	arg1	values					686:691	genome-to-genome distance (GGD) values	654:691	genome-to-genome distance (GGD) values	654:691	Average nucleotide identity (ANI) and genome-to-genome distance (GGD) values between strain MIC A30T and A. liuii CGMCC 1.12778T were 60.34% and 29.39%, respectively.
32661667	5	37	theme	strain	521:526	arg1	A30T					532:535	strain MIC A30T	521:535	strain MIC A30T	521:535	The DNA-DNA relatedness values between strain MIC A30T and these reference strains were below 30%.
32661667	4	38	theme	MIC	302:304	arg1	A30T					306:309	Strain MIC A30T	295:309	Strain MIC A30T	295:309	Strain MIC A30T was moderately related to Arthrobacter liuii CGMCC 1.12778T (97.9%), Arthrobacter pokkaliiT (97.9%) and Arthrobacter globiformis NBRC 12137T (96.7%) by 16S rRNA analysis.
32661667	10	39	theme	Major	892:896	arg1	acids					904:908	Major fatty acids	892:908	Major fatty acids	892:908	Major fatty acids were iso-C15:0, anteiso-C15:0 and anteiso-C17:0.
32661667	7	40	theme	A.	721:722	arg1	1.12778T					736:743	A. liuii CGMCC 1.12778T	721:743	A. liuii CGMCC 1.12778T	721:743	Average nucleotide identity (ANI) and genome-to-genome distance (GGD) values between strain MIC A30T and A. liuii CGMCC 1.12778T were 60.34% and 29.39%, respectively.
32661667	7	41	theme	distance	671:678	arg1	values					686:691	genome-to-genome distance (GGD) values	654:691	genome-to-genome distance (GGD) values	654:691	Average nucleotide identity (ANI) and genome-to-genome distance (GGD) values between strain MIC A30T and A. liuii CGMCC 1.12778T were 60.34% and 29.39%, respectively.
32661667	6	42	theme	DNA	585:587	arg1	content					593:599	The DNA G+C content	581:599	The DNA G+C content	581:599	The DNA G+C content was 63.1 mol%.
32661667	6	42	theme	DNA	585:587	arg1	%					613:613	63.1 mol%	605:613	63.1 mol%	605:613	The DNA G+C content was 63.1 mol%.
32661667	4	43	theme	Strain	295:300	arg1	A30T					306:309	Strain MIC A30T	295:309	Strain MIC A30T	295:309	Strain MIC A30T was moderately related to Arthrobacter liuii CGMCC 1.12778T (97.9%), Arthrobacter pokkaliiT (97.9%) and Arthrobacter globiformis NBRC 12137T (96.7%) by 16S rRNA analysis.
32661667	2	44	theme	MIC	151:153	arg1	strain					143:148	A Gram-stain positive, motile, aerobic and rod-shaped strain	89:148	A Gram-stain positive, motile, aerobic and rod-shaped strain (MIC A30T)	89:159	A Gram-stain positive, motile, aerobic and rod-shaped strain (MIC A30T) was isolated from river sediment in Yuantouzhu park, Wuxi City, China.
32661667	2	44	theme	MIC	151:153	arg1	A30T					155:158	MIC A30T	151:158	MIC A30T	151:158	A Gram-stain positive, motile, aerobic and rod-shaped strain (MIC A30T) was isolated from river sediment in Yuantouzhu park, Wuxi City, China.
32661667	13	45	theme	taxonomic	1137:1145	arg1	results					1147:1153	several taxonomic results	1129:1153	several taxonomic results	1129:1153	Based on several taxonomic results, strain MIC A30T is identified as a novel species in genus Arthrobacter, whose name is proposed as Arthrobacter sedimenti sp.
32661667	7	46	theme	GGD	681:683	arg1	values					686:691	genome-to-genome distance (GGD) values	654:691	genome-to-genome distance (GGD) values	654:691	Average nucleotide identity (ANI) and genome-to-genome distance (GGD) values between strain MIC A30T and A. liuii CGMCC 1.12778T were 60.34% and 29.39%, respectively.
32661667	4	47	theme	Arthrobacter	415:426	arg1	globiformis					428:438	Arthrobacter globiformis	415:438	Arthrobacter globiformis	415:438	Strain MIC A30T was moderately related to Arthrobacter liuii CGMCC 1.12778T (97.9%), Arthrobacter pokkaliiT (97.9%) and Arthrobacter globiformis NBRC 12137T (96.7%) by 16S rRNA analysis.
32661667	13	48	theme	genus	1208:1212	arg1	Arthrobacter					1214:1225	genus Arthrobacter	1208:1225	genus Arthrobacter	1208:1225	Based on several taxonomic results, strain MIC A30T is identified as a novel species in genus Arthrobacter, whose name is proposed as Arthrobacter sedimenti sp.
32661667	5	49	theme	reference	547:555	arg1	strains					557:563	these reference strains	541:563	these reference strains	541:563	The DNA-DNA relatedness values between strain MIC A30T and these reference strains were below 30%.
32661667	2	50	theme	rod-shaped	132:141	arg1	strain					143:148	A Gram-stain positive, motile, aerobic and rod-shaped strain	89:148	A Gram-stain positive, motile, aerobic and rod-shaped strain (MIC A30T)	89:159	A Gram-stain positive, motile, aerobic and rod-shaped strain (MIC A30T) was isolated from river sediment in Yuantouzhu park, Wuxi City, China.
32661667	2	50	theme	rod-shaped	132:141	arg1	A30T					155:158	MIC A30T	151:158	MIC A30T	151:158	A Gram-stain positive, motile, aerobic and rod-shaped strain (MIC A30T) was isolated from river sediment in Yuantouzhu park, Wuxi City, China.
32661667	2	51	theme	river	179:183	arg1	sediment					185:192	river sediment	179:192	river sediment in Yuantouzhu park, Wuxi City, China	179:229	A Gram-stain positive, motile, aerobic and rod-shaped strain (MIC A30T) was isolated from river sediment in Yuantouzhu park, Wuxi City, China.
32661667	9	52	theme	Major	819:823	arg1	lipids					831:836	Major polar lipids	819:836	Major polar lipids	819:836	Major polar lipids were diphosphatidylglycerol and phosphatidylglycerol.
32661667	4	53	theme	NBRC	440:443	arg1	12137T					445:450	NBRC 12137T	440:450	NBRC 12137T	440:450	Strain MIC A30T was moderately related to Arthrobacter liuii CGMCC 1.12778T (97.9%), Arthrobacter pokkaliiT (97.9%) and Arthrobacter globiformis NBRC 12137T (96.7%) by 16S rRNA analysis.
32661667	4	54	theme	Arthrobacter	337:348	arg1	1.12778T					362:369	Arthrobacter liuii CGMCC 1.12778T	337:369	Arthrobacter liuii CGMCC 1.12778T (97.9%)	337:377	Strain MIC A30T was moderately related to Arthrobacter liuii CGMCC 1.12778T (97.9%), Arthrobacter pokkaliiT (97.9%) and Arthrobacter globiformis NBRC 12137T (96.7%) by 16S rRNA analysis.
32661667	4	54	theme	Arthrobacter	337:348	arg1	%					376:376	97.9%	372:376	97.9%	372:376	Strain MIC A30T was moderately related to Arthrobacter liuii CGMCC 1.12778T (97.9%), Arthrobacter pokkaliiT (97.9%) and Arthrobacter globiformis NBRC 12137T (96.7%) by 16S rRNA analysis.
32661667	11	55	theme	Whole-cell	959:968	arg1	sugars					970:975	Whole-cell sugars	959:975	Whole-cell sugars	959:975	Whole-cell sugars were galactose, mannose and rhamnose.
32661667	13	56	from	species	1197:1203	arg1	Arthrobacter					1214:1225	genus Arthrobacter	1208:1225	genus Arthrobacter	1208:1225	Based on several taxonomic results, strain MIC A30T is identified as a novel species in genus Arthrobacter, whose name is proposed as Arthrobacter sedimenti sp.
32661667	9	57	theme	polar	825:829	arg1	lipids					831:836	Major polar lipids	819:836	Major polar lipids	819:836	Major polar lipids were diphosphatidylglycerol and phosphatidylglycerol.
32661667	6	58	theme	63.1 mol	605:612	arg1	content					593:599	The DNA G+C content	581:599	The DNA G+C content	581:599	The DNA G+C content was 63.1 mol%.
32661667	6	58	theme	63.1 mol	605:612	arg1	%					613:613	63.1 mol%	605:613	63.1 mol%	605:613	The DNA G+C content was 63.1 mol%.
32661667	12	59	theme	diamino	1106:1112	arg1	acid					1114:1117	the diagnostic diamino acid	1091:1117	the diagnostic diamino acid	1091:1117	The cell wall peptidoglycan contained A4α peptidoglycan type with lysine as the diagnostic diamino acid.
32661667	12	59	theme	diamino	1106:1112	arg1	type					1071:1074	A4α peptidoglycan type	1053:1074	A4α peptidoglycan type	1053:1074	The cell wall peptidoglycan contained A4α peptidoglycan type with lysine as the diagnostic diamino acid.
32661667	4	60	theme	CGMCC	356:360	arg1	1.12778T					362:369	Arthrobacter liuii CGMCC 1.12778T	337:369	Arthrobacter liuii CGMCC 1.12778T (97.9%)	337:377	Strain MIC A30T was moderately related to Arthrobacter liuii CGMCC 1.12778T (97.9%), Arthrobacter pokkaliiT (97.9%) and Arthrobacter globiformis NBRC 12137T (96.7%) by 16S rRNA analysis.
32661667	4	60	theme	CGMCC	356:360	arg1	%					376:376	97.9%	372:376	97.9%	372:376	Strain MIC A30T was moderately related to Arthrobacter liuii CGMCC 1.12778T (97.9%), Arthrobacter pokkaliiT (97.9%) and Arthrobacter globiformis NBRC 12137T (96.7%) by 16S rRNA analysis.
32661667	12	61	theme	diagnostic	1095:1104	arg1	acid					1114:1117	the diagnostic diamino acid	1091:1117	the diagnostic diamino acid	1091:1117	The cell wall peptidoglycan contained A4α peptidoglycan type with lysine as the diagnostic diamino acid.
32661667	12	61	theme	diagnostic	1095:1104	arg1	type					1071:1074	A4α peptidoglycan type	1053:1074	A4α peptidoglycan type	1053:1074	The cell wall peptidoglycan contained A4α peptidoglycan type with lysine as the diagnostic diamino acid.
32661667	7	62	theme	Average	616:622	arg1	ANI					645:647	ANI	645:647	ANI	645:647	Average nucleotide identity (ANI) and genome-to-genome distance (GGD) values between strain MIC A30T and A. liuii CGMCC 1.12778T were 60.34% and 29.39%, respectively.
32661667	7	62	theme	Average	616:622	arg1	identity					635:642	Average nucleotide identity	616:642	Average nucleotide identity (ANI)	616:648	Average nucleotide identity (ANI) and genome-to-genome distance (GGD) values between strain MIC A30T and A. liuii CGMCC 1.12778T were 60.34% and 29.39%, respectively.
32661667	4	63	theme	liuii	350:354	arg1	1.12778T					362:369	Arthrobacter liuii CGMCC 1.12778T	337:369	Arthrobacter liuii CGMCC 1.12778T (97.9%)	337:377	Strain MIC A30T was moderately related to Arthrobacter liuii CGMCC 1.12778T (97.9%), Arthrobacter pokkaliiT (97.9%) and Arthrobacter globiformis NBRC 12137T (96.7%) by 16S rRNA analysis.
32661667	4	63	theme	liuii	350:354	arg1	%					376:376	97.9%	372:376	97.9%	372:376	Strain MIC A30T was moderately related to Arthrobacter liuii CGMCC 1.12778T (97.9%), Arthrobacter pokkaliiT (97.9%) and Arthrobacter globiformis NBRC 12137T (96.7%) by 16S rRNA analysis.
32661667	15	64	theme	MIC	1305:1307	arg1	strain					1295:1300	The type strain	1286:1300	The type strain	1286:1300	The type strain is MIC A30T (= KACC 19599T = CGMCC 1.13474T).
32661667	15	64	theme	MIC	1305:1307	arg1	A30T					1309:1312	MIC A30T	1305:1312	MIC A30T (= KACC 19599T = CGMCC 1.13474T)	1305:1345	The type strain is MIC A30T (= KACC 19599T = CGMCC 1.13474T).
32661667	15	64	theme	MIC	1305:1307	arg1	1.13474T					1337:1344	= KACC 19599T = CGMCC 1.13474T	1315:1344	= KACC 19599T = CGMCC 1.13474T	1315:1344	The type strain is MIC A30T (= KACC 19599T = CGMCC 1.13474T).
32661667	12	65	theme	wall	1024:1027	arg1	peptidoglycan					1029:1041	The cell wall peptidoglycan	1015:1041	The cell wall peptidoglycan	1015:1041	The cell wall peptidoglycan contained A4α peptidoglycan type with lysine as the diagnostic diamino acid.
32661667	6	66	theme	G+C	589:591	arg1	content					593:599	The DNA G+C content	581:599	The DNA G+C content	581:599	The DNA G+C content was 63.1 mol%.
32661667	6	66	theme	G+C	589:591	arg1	%					613:613	63.1 mol%	605:613	63.1 mol%	605:613	The DNA G+C content was 63.1 mol%.
32661667	12	67	theme	peptidoglycan	1057:1069	arg1	acid					1114:1117	the diagnostic diamino acid	1091:1117	the diagnostic diamino acid	1091:1117	The cell wall peptidoglycan contained A4α peptidoglycan type with lysine as the diagnostic diamino acid.
32661667	12	67	theme	peptidoglycan	1057:1069	arg1	type					1071:1074	A4α peptidoglycan type	1053:1074	A4α peptidoglycan type	1053:1074	The cell wall peptidoglycan contained A4α peptidoglycan type with lysine as the diagnostic diamino acid.
32661667	4	68	dep	related	326:332	arg1	%					457:457	96.7%	453:457	96.7%	453:457	Strain MIC A30T was moderately related to Arthrobacter liuii CGMCC 1.12778T (97.9%), Arthrobacter pokkaliiT (97.9%) and Arthrobacter globiformis NBRC 12137T (96.7%) by 16S rRNA analysis.
32661667	13	69	theme	novel	1191:1195	arg1	A30T					1167:1170	strain MIC A30T	1156:1170	strain MIC A30T	1156:1170	Based on several taxonomic results, strain MIC A30T is identified as a novel species in genus Arthrobacter, whose name is proposed as Arthrobacter sedimenti sp.
32661667	13	69	theme	novel	1191:1195	arg1	species					1197:1203	a novel species	1189:1203	a novel species	1189:1203	Based on several taxonomic results, strain MIC A30T is identified as a novel species in genus Arthrobacter, whose name is proposed as Arthrobacter sedimenti sp.
33775762	5	0	theme	minimum	771:777	arg1	incidence					785:793	minimum decay incidence	771:793	minimum decay incidence	771:793	Significantly synergistic effects were caused by combining C. laurentii and CMC on minimum decay incidence and lesion diameter.
33775762	8	1	theme	commercial	1165:1174	arg1	loss					1263:1266	weight loss	1256:1266	weight loss	1256:1266	Moreover, the commercial quality parameters in the combined treatment of C. laurentii and CMC, including weight loss, total soluble solids, ascorbic acid, and titratable acidity, were superior to single treatment.
33775762	8	1	theme	commercial	1165:1174	arg1	superior					1335:1342	superior	1335:1342	superior	1335:1342	Moreover, the commercial quality parameters in the combined treatment of C. laurentii and CMC, including weight loss, total soluble solids, ascorbic acid, and titratable acidity, were superior to single treatment.
33775762	8	1	theme	commercial	1165:1174	arg1	acidity					1321:1327	titratable acidity	1310:1327	titratable acidity	1310:1327	Moreover, the commercial quality parameters in the combined treatment of C. laurentii and CMC, including weight loss, total soluble solids, ascorbic acid, and titratable acidity, were superior to single treatment.
33775762	8	1	theme	commercial	1165:1174	arg1	solids					1283:1288	total soluble solids	1269:1288	total soluble solids	1269:1288	Moreover, the commercial quality parameters in the combined treatment of C. laurentii and CMC, including weight loss, total soluble solids, ascorbic acid, and titratable acidity, were superior to single treatment.
33775762	8	1	theme	commercial	1165:1174	arg1	parameters					1184:1193	the commercial quality parameters	1161:1193	the commercial quality parameters	1161:1193	Moreover, the commercial quality parameters in the combined treatment of C. laurentii and CMC, including weight loss, total soluble solids, ascorbic acid, and titratable acidity, were superior to single treatment.
33775762	8	1	theme	commercial	1165:1174	arg1	acid					1300:1303	ascorbic acid	1291:1303	ascorbic acid	1291:1303	Moreover, the commercial quality parameters in the combined treatment of C. laurentii and CMC, including weight loss, total soluble solids, ascorbic acid, and titratable acidity, were superior to single treatment.
33775762	1	2	theme	carboxymethylcellulose	171:192	arg1	effects					160:166	The synergistic effects	144:166	The synergistic effects of carboxymethylcellulose (CMC) combined with Cryptococcus laurentii FRUC DJ1	144:244	The synergistic effects of carboxymethylcellulose (CMC) combined with Cryptococcus laurentii FRUC DJ1 were studied on controlling green mould resulting from Penicillium digitatum in grapefruit fruit.
33775762	1	3	theme	green	274:278	arg1	mould					280:284	green mould	274:284	green mould resulting from Penicillium digitatum in grapefruit fruit	274:341	The synergistic effects of carboxymethylcellulose (CMC) combined with Cryptococcus laurentii FRUC DJ1 were studied on controlling green mould resulting from Penicillium digitatum in grapefruit fruit.
33775762	5	4	theme	decay	779:783	arg1	incidence					785:793	minimum decay incidence	771:793	minimum decay incidence	771:793	Significantly synergistic effects were caused by combining C. laurentii and CMC on minimum decay incidence and lesion diameter.
33775762	0	5	theme	grapefruit	114:123	arg1	mould					93:97	green mould	87:97	green mould of postharvest grapefruit	87:123	Synergistic effect of carboxymethylcellulose and Cryptococcus laurentii on suppressing green mould of postharvest grapefruit and its mechanism.
33775762	0	5	theme	grapefruit	114:123	arg1	mechanism					133:141	its mechanism	129:141	its mechanism	129:141	Synergistic effect of carboxymethylcellulose and Cryptococcus laurentii on suppressing green mould of postharvest grapefruit and its mechanism.
33775762	9	6	theme	postharvest	1426:1436	arg1	decay					1438:1442	postharvest decay	1426:1442	postharvest decay	1426:1442	The combination of C. laurentii and CMC can not only control postharvest decay but also maintain fruit qualities.
33775762	0	7	theme	postharvest	102:112	arg1	grapefruit					114:123	postharvest grapefruit	102:123	postharvest grapefruit	102:123	Synergistic effect of carboxymethylcellulose and Cryptococcus laurentii on suppressing green mould of postharvest grapefruit and its mechanism.
33775762	6	8	theme	polyphenol	920:929	arg1	oxidase					931:937	polyphenol oxidase	920:937	polyphenol oxidase	920:937	Combined treatment induced defence enzyme activities, including chitinase, β-1,3-glucanase, peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase, together with disease tolerance-associated total phenol.
33775762	8	9	theme	single	1347:1352	arg1	treatment					1354:1362	single treatment	1347:1362	single treatment	1347:1362	Moreover, the commercial quality parameters in the combined treatment of C. laurentii and CMC, including weight loss, total soluble solids, ascorbic acid, and titratable acidity, were superior to single treatment.
33775762	6	10	theme	Combined	816:823	arg1	treatment					825:833	Combined treatment	816:833	Combined treatment	816:833	Combined treatment induced defence enzyme activities, including chitinase, β-1,3-glucanase, peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase, together with disease tolerance-associated total phenol.
33775762	3	11	theme	CMC	521:523	arg1	concentrations					525:538	low CMC concentrations	517:538	low CMC concentrations	517:538	In addition, C. laurentii growth in vitro was not affected by low CMC concentrations, nevertheless, the biofilm of C. laurentii was enhanced.
33775762	8	12	theme	titratable	1310:1319	arg1	acidity					1321:1327	titratable acidity	1310:1327	titratable acidity	1310:1327	Moreover, the commercial quality parameters in the combined treatment of C. laurentii and CMC, including weight loss, total soluble solids, ascorbic acid, and titratable acidity, were superior to single treatment.
33775762	3	13	theme	C.	468:469	arg1	growth					481:486	C. laurentii growth	468:486	C. laurentii growth in vitro	468:495	In addition, C. laurentii growth in vitro was not affected by low CMC concentrations, nevertheless, the biofilm of C. laurentii was enhanced.
33775762	8	14	theme	combined	1202:1209	arg1	treatment					1211:1219	the combined treatment	1198:1219	the combined treatment of C. laurentii and CMC	1198:1243	Moreover, the commercial quality parameters in the combined treatment of C. laurentii and CMC, including weight loss, total soluble solids, ascorbic acid, and titratable acidity, were superior to single treatment.
33775762	7	15	theme	fruit	1137:1141	arg1	wounds					1143:1148	fruit wounds	1137:1148	fruit wounds	1137:1148	Also, this combination inhibited the pathogen growth by adhered to the hyphae and reduced its infection in fruit wounds.
33775762	9	16	theme	CMC	1401:1403	arg1	combination					1369:1379	The combination	1365:1379	The combination of C. laurentii and CMC	1365:1403	The combination of C. laurentii and CMC can not only control postharvest decay but also maintain fruit qualities.
33775762	2	17	theme	P.	421:422	arg1	conidia					434:440	P. digitatum conidia	421:440	P. digitatum conidia germination	421:452	The results indicate that both C. laurentii and the CMC treatment suppressed P. digitatum conidia germination.
33775762	8	18	theme	C.	1224:1225	arg1	laurentii					1227:1235	C. laurentii	1224:1235	C. laurentii	1224:1235	Moreover, the commercial quality parameters in the combined treatment of C. laurentii and CMC, including weight loss, total soluble solids, ascorbic acid, and titratable acidity, were superior to single treatment.
33775762	6	19	theme	disease	987:993	arg1	phenol					1022:1027	disease tolerance-associated total phenol	987:1027	disease tolerance-associated total phenol	987:1027	Combined treatment induced defence enzyme activities, including chitinase, β-1,3-glucanase, peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase, together with disease tolerance-associated total phenol.
33775762	4	20	theme	green	657:661	arg1	mould					663:667	a lower green mould	649:667	a lower green mould	649:667	Compared with the control fruit, the grapefruit had a lower green mould in all treatments.
33775762	8	21	theme	soluble	1275:1281	arg1	solids					1283:1288	total soluble solids	1269:1288	total soluble solids	1269:1288	Moreover, the commercial quality parameters in the combined treatment of C. laurentii and CMC, including weight loss, total soluble solids, ascorbic acid, and titratable acidity, were superior to single treatment.
33775762	0	22	theme	Synergistic	0:10	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of carboxymethylcellulose and Cryptococcus laurentii on suppressing green mould of postharvest grapefruit and its mechanism.	0:142	Synergistic effect of carboxymethylcellulose and Cryptococcus laurentii on suppressing green mould of postharvest grapefruit and its mechanism.
33775762	8	23	theme	total	1269:1273	arg1	solids					1283:1288	total soluble solids	1269:1288	total soluble solids	1269:1288	Moreover, the commercial quality parameters in the combined treatment of C. laurentii and CMC, including weight loss, total soluble solids, ascorbic acid, and titratable acidity, were superior to single treatment.
33775762	0	24	theme	carboxymethylcellulose	22:43	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of carboxymethylcellulose and Cryptococcus laurentii on suppressing green mould of postharvest grapefruit and its mechanism.	0:142	Synergistic effect of carboxymethylcellulose and Cryptococcus laurentii on suppressing green mould of postharvest grapefruit and its mechanism.
33775762	6	25	theme	total	1016:1020	arg1	phenol					1022:1027	disease tolerance-associated total phenol	987:1027	disease tolerance-associated total phenol	987:1027	Combined treatment induced defence enzyme activities, including chitinase, β-1,3-glucanase, peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase, together with disease tolerance-associated total phenol.
33775762	3	26	theme	C.	570:571	arg1	laurentii					573:581	C. laurentii	570:581	C. laurentii	570:581	In addition, C. laurentii growth in vitro was not affected by low CMC concentrations, nevertheless, the biofilm of C. laurentii was enhanced.
33775762	1	27	theme	Cryptococcus	214:225	arg1	DJ1					242:244	Cryptococcus laurentii FRUC DJ1	214:244	Cryptococcus laurentii FRUC DJ1	214:244	The synergistic effects of carboxymethylcellulose (CMC) combined with Cryptococcus laurentii FRUC DJ1 were studied on controlling green mould resulting from Penicillium digitatum in grapefruit fruit.
33775762	8	28	theme	laurentii	1227:1235	arg1	treatment					1211:1219	the combined treatment	1198:1219	the combined treatment of C. laurentii and CMC	1198:1243	Moreover, the commercial quality parameters in the combined treatment of C. laurentii and CMC, including weight loss, total soluble solids, ascorbic acid, and titratable acidity, were superior to single treatment.
33775762	6	29	theme	tolerance-associated	995:1014	arg1	phenol					1022:1027	disease tolerance-associated total phenol	987:1027	disease tolerance-associated total phenol	987:1027	Combined treatment induced defence enzyme activities, including chitinase, β-1,3-glucanase, peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase, together with disease tolerance-associated total phenol.
33775762	8	30	from	parameters	1184:1193	arg1	treatment					1211:1219	the combined treatment	1198:1219	the combined treatment of C. laurentii and CMC	1198:1243	Moreover, the commercial quality parameters in the combined treatment of C. laurentii and CMC, including weight loss, total soluble solids, ascorbic acid, and titratable acidity, were superior to single treatment.
33775762	3	31	theme	laurentii	573:581	arg1	biofilm					559:565	the biofilm	555:565	the biofilm of C. laurentii	555:581	In addition, C. laurentii growth in vitro was not affected by low CMC concentrations, nevertheless, the biofilm of C. laurentii was enhanced.
33775762	1	32	theme	laurentii	227:235	arg1	DJ1					242:244	Cryptococcus laurentii FRUC DJ1	214:244	Cryptococcus laurentii FRUC DJ1	214:244	The synergistic effects of carboxymethylcellulose (CMC) combined with Cryptococcus laurentii FRUC DJ1 were studied on controlling green mould resulting from Penicillium digitatum in grapefruit fruit.
33775762	0	33	theme	Cryptococcus	49:60	arg1	laurentii					62:70	Cryptococcus laurentii	49:70	Cryptococcus laurentii	49:70	Synergistic effect of carboxymethylcellulose and Cryptococcus laurentii on suppressing green mould of postharvest grapefruit and its mechanism.
33775762	1	34	theme	grapefruit	326:335	arg1	fruit					337:341	grapefruit fruit	326:341	grapefruit fruit	326:341	The synergistic effects of carboxymethylcellulose (CMC) combined with Cryptococcus laurentii FRUC DJ1 were studied on controlling green mould resulting from Penicillium digitatum in grapefruit fruit.
33775762	4	35	theme	lower	651:655	arg1	mould					663:667	a lower green mould	649:667	a lower green mould	649:667	Compared with the control fruit, the grapefruit had a lower green mould in all treatments.
33775762	9	36	theme	fruit	1462:1466	arg1	qualities					1468:1476	fruit qualities	1462:1476	fruit qualities	1462:1476	The combination of C. laurentii and CMC can not only control postharvest decay but also maintain fruit qualities.
33775762	1	37	theme	FRUC	237:240	arg1	DJ1					242:244	Cryptococcus laurentii FRUC DJ1	214:244	Cryptococcus laurentii FRUC DJ1	214:244	The synergistic effects of carboxymethylcellulose (CMC) combined with Cryptococcus laurentii FRUC DJ1 were studied on controlling green mould resulting from Penicillium digitatum in grapefruit fruit.
33775762	8	38	theme	CMC	1241:1243	arg1	treatment					1211:1219	the combined treatment	1198:1219	the combined treatment of C. laurentii and CMC	1198:1243	Moreover, the commercial quality parameters in the combined treatment of C. laurentii and CMC, including weight loss, total soluble solids, ascorbic acid, and titratable acidity, were superior to single treatment.
33775762	8	39	theme	quality	1176:1182	arg1	loss					1263:1266	weight loss	1256:1266	weight loss	1256:1266	Moreover, the commercial quality parameters in the combined treatment of C. laurentii and CMC, including weight loss, total soluble solids, ascorbic acid, and titratable acidity, were superior to single treatment.
33775762	8	39	theme	quality	1176:1182	arg1	superior					1335:1342	superior	1335:1342	superior	1335:1342	Moreover, the commercial quality parameters in the combined treatment of C. laurentii and CMC, including weight loss, total soluble solids, ascorbic acid, and titratable acidity, were superior to single treatment.
33775762	8	39	theme	quality	1176:1182	arg1	acidity					1321:1327	titratable acidity	1310:1327	titratable acidity	1310:1327	Moreover, the commercial quality parameters in the combined treatment of C. laurentii and CMC, including weight loss, total soluble solids, ascorbic acid, and titratable acidity, were superior to single treatment.
33775762	8	39	theme	quality	1176:1182	arg1	solids					1283:1288	total soluble solids	1269:1288	total soluble solids	1269:1288	Moreover, the commercial quality parameters in the combined treatment of C. laurentii and CMC, including weight loss, total soluble solids, ascorbic acid, and titratable acidity, were superior to single treatment.
33775762	8	39	theme	quality	1176:1182	arg1	parameters					1184:1193	the commercial quality parameters	1161:1193	the commercial quality parameters	1161:1193	Moreover, the commercial quality parameters in the combined treatment of C. laurentii and CMC, including weight loss, total soluble solids, ascorbic acid, and titratable acidity, were superior to single treatment.
33775762	8	39	theme	quality	1176:1182	arg1	acid					1300:1303	ascorbic acid	1291:1303	ascorbic acid	1291:1303	Moreover, the commercial quality parameters in the combined treatment of C. laurentii and CMC, including weight loss, total soluble solids, ascorbic acid, and titratable acidity, were superior to single treatment.
33775762	2	40	theme	conidia	434:440	arg1	germination					442:452	P. digitatum conidia germination	421:452	P. digitatum conidia germination	421:452	The results indicate that both C. laurentii and the CMC treatment suppressed P. digitatum conidia germination.
33775762	5	41	theme	lesion	799:804	arg1	diameter					806:813	lesion diameter	799:813	lesion diameter	799:813	Significantly synergistic effects were caused by combining C. laurentii and CMC on minimum decay incidence and lesion diameter.
33775762	10	42	theme	commercial	1518:1527	arg1	purposes					1529:1536	commercial purposes	1518:1536	commercial purposes	1518:1536	Thus, it can be used in grapefruit for commercial purposes.
33775762	4	43	contain	had	645:647	arg1	grapefruit					634:643	the grapefruit	630:643	the grapefruit	630:643	Compared with the control fruit, the grapefruit had a lower green mould in all treatments.
33775762	4	43	contain	had	645:647	arg2	mould					663:667	a lower green mould	649:667	a lower green mould	649:667	Compared with the control fruit, the grapefruit had a lower green mould in all treatments.
33775762	7	44	theme	pathogen	1067:1074	arg1	growth					1076:1081	the pathogen growth	1063:1081	the pathogen growth	1063:1081	Also, this combination inhibited the pathogen growth by adhered to the hyphae and reduced its infection in fruit wounds.
33775762	2	45	theme	C.	375:376	arg1	laurentii					378:386	C. laurentii	375:386	C. laurentii	375:386	The results indicate that both C. laurentii and the CMC treatment suppressed P. digitatum conidia germination.
33775762	0	46	theme	laurentii	62:70	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of carboxymethylcellulose and Cryptococcus laurentii on suppressing green mould of postharvest grapefruit and its mechanism.	0:142	Synergistic effect of carboxymethylcellulose and Cryptococcus laurentii on suppressing green mould of postharvest grapefruit and its mechanism.
33775762	9	47	theme	C.	1384:1385	arg1	laurentii					1387:1395	C. laurentii	1384:1395	C. laurentii	1384:1395	The combination of C. laurentii and CMC can not only control postharvest decay but also maintain fruit qualities.
33775762	3	48	theme	low	517:519	arg1	concentrations					525:538	low CMC concentrations	517:538	low CMC concentrations	517:538	In addition, C. laurentii growth in vitro was not affected by low CMC concentrations, nevertheless, the biofilm of C. laurentii was enhanced.
33775762	9	49	theme	laurentii	1387:1395	arg1	combination					1369:1379	The combination	1365:1379	The combination of C. laurentii and CMC	1365:1403	The combination of C. laurentii and CMC can not only control postharvest decay but also maintain fruit qualities.
33775762	8	50	theme	ascorbic	1291:1298	arg1	acid					1300:1303	ascorbic acid	1291:1303	ascorbic acid	1291:1303	Moreover, the commercial quality parameters in the combined treatment of C. laurentii and CMC, including weight loss, total soluble solids, ascorbic acid, and titratable acidity, were superior to single treatment.
33775762	5	51	theme	synergistic	702:712	arg1	effects					714:720	synergistic effects	702:720	synergistic effects	702:720	Significantly synergistic effects were caused by combining C. laurentii and CMC on minimum decay incidence and lesion diameter.
33775762	6	52	theme	enzyme	851:856	arg1	activities					858:867	defence enzyme activities	843:867	defence enzyme activities	843:867	Combined treatment induced defence enzyme activities, including chitinase, β-1,3-glucanase, peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase, together with disease tolerance-associated total phenol.
33775762	6	52	theme	enzyme	851:856	arg1	peroxidase					908:917	peroxidase	908:917	peroxidase	908:917	Combined treatment induced defence enzyme activities, including chitinase, β-1,3-glucanase, peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase, together with disease tolerance-associated total phenol.
33775762	6	52	theme	enzyme	851:856	arg1	ammonia-lyase					958:970	phenylalanine ammonia-lyase	944:970	phenylalanine ammonia-lyase	944:970	Combined treatment induced defence enzyme activities, including chitinase, β-1,3-glucanase, peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase, together with disease tolerance-associated total phenol.
33775762	6	52	theme	enzyme	851:856	arg1	chitinase					880:888	chitinase	880:888	chitinase	880:888	Combined treatment induced defence enzyme activities, including chitinase, β-1,3-glucanase, peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase, together with disease tolerance-associated total phenol.
33775762	6	52	theme	enzyme	851:856	arg1	oxidase					931:937	polyphenol oxidase	920:937	polyphenol oxidase	920:937	Combined treatment induced defence enzyme activities, including chitinase, β-1,3-glucanase, peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase, together with disease tolerance-associated total phenol.
33775762	6	52	theme	enzyme	851:856	arg1	β-1,3-glucanase					891:905	β-1,3-glucanase	891:905	β-1,3-glucanase	891:905	Combined treatment induced defence enzyme activities, including chitinase, β-1,3-glucanase, peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase, together with disease tolerance-associated total phenol.
33775762	1	53	theme	synergistic	148:158	arg1	effects					160:166	The synergistic effects	144:166	The synergistic effects of carboxymethylcellulose (CMC) combined with Cryptococcus laurentii FRUC DJ1	144:244	The synergistic effects of carboxymethylcellulose (CMC) combined with Cryptococcus laurentii FRUC DJ1 were studied on controlling green mould resulting from Penicillium digitatum in grapefruit fruit.
33775762	4	54	theme	control	615:621	arg1	fruit					623:627	the control fruit	611:627	the control fruit	611:627	Compared with the control fruit, the grapefruit had a lower green mould in all treatments.
33775762	0	55	theme	green	87:91	arg1	mould					93:97	green mould	87:97	green mould of postharvest grapefruit	87:123	Synergistic effect of carboxymethylcellulose and Cryptococcus laurentii on suppressing green mould of postharvest grapefruit and its mechanism.
33775762	6	56	theme	defence	843:849	arg1	activities					858:867	defence enzyme activities	843:867	defence enzyme activities	843:867	Combined treatment induced defence enzyme activities, including chitinase, β-1,3-glucanase, peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase, together with disease tolerance-associated total phenol.
33775762	6	56	theme	defence	843:849	arg1	peroxidase					908:917	peroxidase	908:917	peroxidase	908:917	Combined treatment induced defence enzyme activities, including chitinase, β-1,3-glucanase, peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase, together with disease tolerance-associated total phenol.
33775762	6	56	theme	defence	843:849	arg1	ammonia-lyase					958:970	phenylalanine ammonia-lyase	944:970	phenylalanine ammonia-lyase	944:970	Combined treatment induced defence enzyme activities, including chitinase, β-1,3-glucanase, peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase, together with disease tolerance-associated total phenol.
33775762	6	56	theme	defence	843:849	arg1	chitinase					880:888	chitinase	880:888	chitinase	880:888	Combined treatment induced defence enzyme activities, including chitinase, β-1,3-glucanase, peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase, together with disease tolerance-associated total phenol.
33775762	6	56	theme	defence	843:849	arg1	oxidase					931:937	polyphenol oxidase	920:937	polyphenol oxidase	920:937	Combined treatment induced defence enzyme activities, including chitinase, β-1,3-glucanase, peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase, together with disease tolerance-associated total phenol.
33775762	6	56	theme	defence	843:849	arg1	β-1,3-glucanase					891:905	β-1,3-glucanase	891:905	β-1,3-glucanase	891:905	Combined treatment induced defence enzyme activities, including chitinase, β-1,3-glucanase, peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase, together with disease tolerance-associated total phenol.
33775762	2	57	theme	CMC	396:398	arg1	treatment					400:408	the CMC treatment	392:408	the CMC treatment	392:408	The results indicate that both C. laurentii and the CMC treatment suppressed P. digitatum conidia germination.
33775762	3	58	theme	laurentii	471:479	arg1	growth					481:486	C. laurentii growth	468:486	C. laurentii growth in vitro	468:495	In addition, C. laurentii growth in vitro was not affected by low CMC concentrations, nevertheless, the biofilm of C. laurentii was enhanced.
33775762	8	59	theme	weight	1256:1261	arg1	loss					1263:1266	weight loss	1256:1266	weight loss	1256:1266	Moreover, the commercial quality parameters in the combined treatment of C. laurentii and CMC, including weight loss, total soluble solids, ascorbic acid, and titratable acidity, were superior to single treatment.
33775762	6	60	theme	phenylalanine	944:956	arg1	ammonia-lyase					958:970	phenylalanine ammonia-lyase	944:970	phenylalanine ammonia-lyase	944:970	Combined treatment induced defence enzyme activities, including chitinase, β-1,3-glucanase, peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase, together with disease tolerance-associated total phenol.
33775762	2	61	dep	P.	421:422	arg1	digitatum					424:432	digitatum	424:432	digitatum	424:432	The results indicate that both C. laurentii and the CMC treatment suppressed P. digitatum conidia germination.
33775762	10	62	used	used	1495:1498	arg2	it					1485:1486	it	1485:1486	it	1485:1486	Thus, it can be used in grapefruit for commercial purposes.
33775762	5	63	theme	C.	747:748	arg1	laurentii					750:758	C. laurentii	747:758	C. laurentii	747:758	Significantly synergistic effects were caused by combining C. laurentii and CMC on minimum decay incidence and lesion diameter.
32493269	7	0	located	observed	999:1006	arg2	growth					988:993	The accelerated growth	972:993	The accelerated growth	972:993	The accelerated growth was observed also in the transgenic line Xeg-1-1c.
32493269	7	0	located	observed	999:1006	arg1	Xeg-1-1c					1036:1043	the transgenic line Xeg-1-1c	1016:1043	the transgenic line Xeg-1-1c	1016:1043	The accelerated growth was observed also in the transgenic line Xeg-1-1c.
32493269	4	1	theme	transgenic	461:470	arg1	aspens					472:477	the transgenic aspens	457:477	the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions	457:602	RESULTS Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time.
32493269	5	2	theme	composition	674:684	arg1	Change					651:656	Change	651:656	Change of carbohydrate composition of wood	651:692	Change of carbohydrate composition of wood was observed in transgenic aspens carrying the sp-Xeg gene.
32493269	9	3	theme	transgenic	1219:1228	arg1	stems					1236:1240	the transgenic aspen stems	1215:1240	the transgenic aspen stems	1215:1240	Undescribed earlier considerable reduction in the wood decomposition rate of the transgenic aspen stems was also revealed for the transformed transgenic lines.
32493269	2	4	theme	open	297:300	arg1	field					302:306	open field	297:306	open field	297:306	However, cultivation of such plants in open field is challenging due to a number of problems.
32493269	6	5	from	outside	910:916	arg1	comparison					921:930	comparison	921:930	comparison with the control untransformed line	921:966	The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt.
32493269	9	6	theme	stems	1236:1240	arg1	rate					1207:1210	the wood decomposition rate	1184:1210	the wood decomposition rate of the transgenic aspen stems	1184:1240	Undescribed earlier considerable reduction in the wood decomposition rate of the transgenic aspen stems was also revealed for the transformed transgenic lines.
32493269	2	7	theme	plants	287:292	arg1	cultivation					267:277	cultivation	267:277	cultivation of such plants in open field	267:306	However, cultivation of such plants in open field is challenging due to a number of problems.
32493269	13	8	dep	the	1750:1752	arg1	leave					1754:1758	leave	1754:1758	leave traits	1754:1765	All lines showed phenotypic changes in the leave traits.
32493269	4	9	theme	Penicillium	555:565	arg1	canescens					567:575	Penicillium canescens	555:575	Penicillium canescens	555:575	RESULTS Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time.
32493269	1	10	theme	transgenic	201:210	arg1	plants					218:223	transgenic woody plants	201:223	transgenic woody plants with improved phenotypic traits	201:255	BACKGROUND Recombinant carbohydrases genes are used to produce transgenic woody plants with improved phenotypic traits.
32493269	9	11	from	reduction	1171:1179	arg1	rate					1207:1210	the wood decomposition rate	1184:1210	the wood decomposition rate of the transgenic aspen stems	1184:1240	Undescribed earlier considerable reduction in the wood decomposition rate of the transgenic aspen stems was also revealed for the transformed transgenic lines.
32493269	5	12	theme	transgenic	710:719	arg1	aspens					721:726	transgenic aspens	710:726	transgenic aspens carrying the sp-Xeg gene	710:751	Change of carbohydrate composition of wood was observed in transgenic aspens carrying the sp-Xeg gene.
32493269	5	13	located	observed	698:705	arg1	aspens					721:726	transgenic aspens	710:726	transgenic aspens carrying the sp-Xeg gene	710:751	Change of carbohydrate composition of wood was observed in transgenic aspens carrying the sp-Xeg gene.
32493269	5	13	located	observed	698:705	arg2	Change					651:656	Change	651:656	Change of carbohydrate composition of wood	651:692	Change of carbohydrate composition of wood was observed in transgenic aspens carrying the sp-Xeg gene.
32493269	12	14	theme	pronounced	1645:1654	arg1	expression					1624:1633	the sp-Xeg gene expression	1608:1633	the sp-Xeg gene expression	1608:1633	The higher was the level of the sp-Xeg gene expression, the more pronounced were changes in the phenotypic and biochemical traits.
32493269	12	14	theme	pronounced	1645:1654	arg1	changes					1661:1667	the more pronounced were changes	1636:1667	the more pronounced were changes in the phenotypic and biochemical traits	1636:1708	The higher was the level of the sp-Xeg gene expression, the more pronounced were changes in the phenotypic and biochemical traits.
32493269	15	15	theme	transgenic	1927:1936	arg1	lines					1938:1942	some transgenic lines	1922:1942	some transgenic lines	1922:1942	In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness, and a decrease in the rate of decomposition of wood were observed.
32493269	9	16	theme	transformed	1268:1278	arg1	lines					1291:1295	the transformed transgenic lines	1264:1295	the transformed transgenic lines	1264:1295	Undescribed earlier considerable reduction in the wood decomposition rate of the transgenic aspen stems was also revealed for the transformed transgenic lines.
32493269	1	17	theme	improved	230:237	arg1	traits					250:255	improved phenotypic traits	230:255	improved phenotypic traits	230:255	BACKGROUND Recombinant carbohydrases genes are used to produce transgenic woody plants with improved phenotypic traits.
32493269	12	18	theme	sp-Xeg	1612:1617	arg1	expression					1624:1633	the sp-Xeg gene expression	1608:1633	the sp-Xeg gene expression	1608:1633	The higher was the level of the sp-Xeg gene expression, the more pronounced were changes in the phenotypic and biochemical traits.
32493269	12	18	theme	sp-Xeg	1612:1617	arg1	changes					1661:1667	the more pronounced were changes	1636:1667	the more pronounced were changes in the phenotypic and biochemical traits	1636:1708	The higher was the level of the sp-Xeg gene expression, the more pronounced were changes in the phenotypic and biochemical traits.
32493269	11	19	dep	CONCLUSION	1441:1450	arg1	demonstrated					1566:1577	demonstrated	1566:1577	was demonstrated	1562:1577	CONCLUSION A direct dependence of the phenotypic and biochemical traits on the expression of the recombinant gene sp-Xeg was demonstrated.
32493269	6	20	dep	Pt	968:969	arg1	outside					910:916	outside	910:916	outside	910:916	The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt.
32493269	6	20	dep	Pt	968:969	arg1	both					890:893	both	890:893	both	890:893	The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt.
32493269	8	21	located	observed	1097:1104	arg1	lines					1131:1135	these transgenic lines	1114:1135	both these transgenic lines	1109:1135	Thicker cell-wall and longer xylem fiber were also observed in both these transgenic lines.
32493269	8	21	located	observed	1097:1104	arg2	fiber					1081:1085	longer xylem fiber	1068:1085	longer xylem fiber	1068:1085	Thicker cell-wall and longer xylem fiber were also observed in both these transgenic lines.
32493269	8	21	located	observed	1097:1104	arg2	cell-wall					1054:1062	Thicker cell-wall	1046:1062	Thicker cell-wall	1046:1062	Thicker cell-wall and longer xylem fiber were also observed in both these transgenic lines.
32493269	5	22	theme	wood	689:692	arg1	composition					674:684	carbohydrate composition	661:684	carbohydrate composition of wood	661:692	Change of carbohydrate composition of wood was observed in transgenic aspens carrying the sp-Xeg gene.
32493269	7	23	theme	transgenic	1020:1029	arg1	Xeg-1-1c					1036:1043	the transgenic line Xeg-1-1c	1016:1043	the transgenic line Xeg-1-1c	1016:1043	The accelerated growth was observed also in the transgenic line Xeg-1-1c.
32493269	14	24	theme	semi-natural	1894:1905	arg1	conditions					1907:1916	semi-natural conditions	1894:1916	semi-natural conditions	1894:1916	Our results showed that the plants carrying the recombinant sp-Xeg gene do not demonstrate a decrease in growth parameters in semi-natural conditions.
32493269	6	25	theme	untransformed	949:961	arg1	line					963:966	the control untransformed line	937:966	the control untransformed line	937:966	The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt.
32493269	11	26	theme	recombinant	1538:1548	arg1	sp-Xeg					1555:1560	the recombinant gene sp-Xeg	1534:1560	the recombinant gene sp-Xeg	1534:1560	CONCLUSION A direct dependence of the phenotypic and biochemical traits on the expression of the recombinant gene sp-Xeg was demonstrated.
32493269	1	27	theme	BACKGROUND	138:147	arg1	genes					175:179	BACKGROUND Recombinant carbohydrases genes	138:179	BACKGROUND Recombinant carbohydrases genes	138:179	BACKGROUND Recombinant carbohydrases genes are used to produce transgenic woody plants with improved phenotypic traits.
32493269	4	28	theme	successful	431:440	arg1	cultivation					442:452	successful cultivation	431:452	successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions	431:602	RESULTS Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time.
32493269	0	29	theme	gene	55:58	arg1	expression					23:32	the expression	19:32	the expression of the xyloglucanase gene	19:58	Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions.
32493269	1	30	theme	carbohydrases	161:173	arg1	genes					175:179	BACKGROUND Recombinant carbohydrases genes	138:179	BACKGROUND Recombinant carbohydrases genes	138:179	BACKGROUND Recombinant carbohydrases genes are used to produce transgenic woody plants with improved phenotypic traits.
32493269	11	31	from	dependence	1461:1470	arg1	expression					1520:1529	the expression	1516:1529	the expression of the recombinant gene sp-Xeg	1516:1560	CONCLUSION A direct dependence of the phenotypic and biochemical traits on the expression of the recombinant gene sp-Xeg was demonstrated.
32493269	0	32	theme	Penicillium	65:75	arg1	canescens					77:85	Penicillium canescens	65:85	Penicillium canescens in transgenic	65:99	Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions.
32493269	8	33	theme	Thicker	1046:1052	arg1	cell-wall					1054:1062	Thicker cell-wall	1046:1062	Thicker cell-wall	1046:1062	Thicker cell-wall and longer xylem fiber were also observed in both these transgenic lines.
32493269	4	34	theme	recombinant	510:520	arg1	sp-Xeg					542:547	sp-Xeg	542:547	sp-Xeg	542:547	RESULTS Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time.
32493269	4	34	theme	recombinant	510:520	arg1	gene					536:539	the recombinant xyloglucanase gene	506:539	the recombinant xyloglucanase gene (sp-Xeg)	506:548	RESULTS Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time.
32493269	6	35	theme	increased	831:839	arg1	content					841:847	increased content	831:847	increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt	831:969	The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt.
32493269	11	36	theme	sp-Xeg	1555:1560	arg1	expression					1520:1529	the expression	1516:1529	the expression of the recombinant gene sp-Xeg	1516:1560	CONCLUSION A direct dependence of the phenotypic and biochemical traits on the expression of the recombinant gene sp-Xeg was demonstrated.
32493269	5	37	theme	sp-Xeg	741:746	arg1	gene					748:751	the sp-Xeg gene	737:751	the sp-Xeg gene	737:751	Change of carbohydrate composition of wood was observed in transgenic aspens carrying the sp-Xeg gene.
32493269	15	38	theme	decomposition	2069:2081	arg1	rate					2061:2064	the rate	2057:2064	the rate of decomposition of wood	2057:2089	In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness, and a decrease in the rate of decomposition of wood were observed.
32493269	12	39	theme	biochemical	1691:1701	arg1	traits					1703:1708	the phenotypic and biochemical traits	1672:1708	the phenotypic and biochemical traits	1672:1708	The higher was the level of the sp-Xeg gene expression, the more pronounced were changes in the phenotypic and biochemical traits.
32493269	15	40	theme	carbohydrate	1961:1972	arg1	composition					1974:1984	the carbohydrate composition	1957:1984	the carbohydrate composition of the wood	1957:1996	In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness, and a decrease in the rate of decomposition of wood were observed.
32493269	8	41	theme	xylem	1075:1079	arg1	fiber					1081:1085	longer xylem fiber	1068:1085	longer xylem fiber	1068:1085	Thicker cell-wall and longer xylem fiber were also observed in both these transgenic lines.
32493269	0	42	theme	Various	0:6	arg1	effects					8:14	Various effects	0:14	Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic	0:99	Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions.
32493269	2	43	from	cultivation	267:277	arg1	field					302:306	open field	297:306	open field	297:306	However, cultivation of such plants in open field is challenging due to a number of problems.
32493269	6	44	from	content	841:847	arg1	wood					865:868	wood	865:868	wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt	865:969	The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt.
32493269	10	45	theme	untransformed	1418:1430	arg1	Pt					1437:1438	the control untransformed line Pt	1406:1438	the control untransformed line Pt	1406:1438	The decomposition rate was approximately twice as lower for the transgenic line Xeg-2-3b in comparison with the control untransformed line Pt.
32493269	11	46	theme	traits	1506:1511	arg1	dependence					1461:1470	A direct dependence	1452:1470	A direct dependence of the phenotypic and biochemical traits on the expression of the recombinant gene sp-Xeg	1452:1560	CONCLUSION A direct dependence of the phenotypic and biochemical traits on the expression of the recombinant gene sp-Xeg was demonstrated.
32493269	12	47	theme	phenotypic	1676:1685	arg1	traits					1703:1708	the phenotypic and biochemical traits	1672:1708	the phenotypic and biochemical traits	1672:1708	The higher was the level of the sp-Xeg gene expression, the more pronounced were changes in the phenotypic and biochemical traits.
32493269	14	48	theme	growth	1873:1878	arg1	parameters					1880:1889	growth parameters	1873:1889	growth parameters in semi-natural conditions	1873:1916	Our results showed that the plants carrying the recombinant sp-Xeg gene do not demonstrate a decrease in growth parameters in semi-natural conditions.
32493269	15	49	theme	cell	2018:2021	arg1	thickness					2028:2036	the cell wall thickness	2014:2036	the cell wall thickness	2014:2036	In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness, and a decrease in the rate of decomposition of wood were observed.
32493269	9	50	theme	Undescribed	1138:1148	arg1	reduction					1171:1179	Undescribed earlier considerable reduction	1138:1179	Undescribed earlier considerable reduction in the wood decomposition rate of the transgenic aspen stems	1138:1240	Undescribed earlier considerable reduction in the wood decomposition rate of the transgenic aspen stems was also revealed for the transformed transgenic lines.
32493269	0	51	theme	expression	23:32	arg1	effects					8:14	Various effects	0:14	Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic	0:99	Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions.
32493269	14	52	theme	sp-Xeg	1828:1833	arg1	gene					1835:1838	the recombinant sp-Xeg gene	1812:1838	the recombinant sp-Xeg gene	1812:1838	Our results showed that the plants carrying the recombinant sp-Xeg gene do not demonstrate a decrease in growth parameters in semi-natural conditions.
32493269	1	53	with	plants	218:223	arg1	traits					250:255	improved phenotypic traits	230:255	improved phenotypic traits	230:255	BACKGROUND Recombinant carbohydrases genes are used to produce transgenic woody plants with improved phenotypic traits.
32493269	6	54	theme	transgenic	770:779	arg1	Xeg-2-1b					786:793	The transformed transgenic line Xeg-2-1b	754:793	The transformed transgenic line Xeg-2-1b	754:793	The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt.
32493269	2	55	theme	problems	342:349	arg1	number					332:337	a number	330:337	a number of problems	330:349	However, cultivation of such plants in open field is challenging due to a number of problems.
32493269	13	56	from	changes	1739:1745	arg1	the					1750:1752	the	1750:1752	the	1750:1752	All lines showed phenotypic changes in the leave traits.
32493269	8	57	theme	transgenic	1120:1129	arg1	lines					1131:1135	these transgenic lines	1114:1135	both these transgenic lines	1109:1135	Thicker cell-wall and longer xylem fiber were also observed in both these transgenic lines.
32493269	6	58	with	comparison	921:930	arg1	line					963:966	the control untransformed line	937:966	the control untransformed line	937:966	The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt.
32493269	10	59	theme	transgenic	1362:1371	arg1	Xeg-2-3b					1378:1385	the transgenic line Xeg-2-3b	1358:1385	the transgenic line Xeg-2-3b	1358:1385	The decomposition rate was approximately twice as lower for the transgenic line Xeg-2-3b in comparison with the control untransformed line Pt.
32493269	14	60	from	parameters	1880:1889	arg1	conditions					1907:1916	semi-natural conditions	1894:1916	semi-natural conditions	1894:1916	Our results showed that the plants carrying the recombinant sp-Xeg gene do not demonstrate a decrease in growth parameters in semi-natural conditions.
32493269	1	61	theme	phenotypic	239:248	arg1	traits					250:255	improved phenotypic traits	230:255	improved phenotypic traits	230:255	BACKGROUND Recombinant carbohydrases genes are used to produce transgenic woody plants with improved phenotypic traits.
32493269	15	62	from	decrease	2045:2052	arg1	rate					2061:2064	the rate	2057:2064	the rate of decomposition of wood	2057:2089	In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness, and a decrease in the rate of decomposition of wood were observed.
32493269	15	62	from	decrease	2045:2052	arg1	composition					1974:1984	the carbohydrate composition	1957:1984	the carbohydrate composition of the wood	1957:1996	In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness, and a decrease in the rate of decomposition of wood were observed.
32493269	15	62	from	decrease	2045:2052	arg1	thickness					2028:2036	the cell wall thickness	2014:2036	the cell wall thickness	2014:2036	In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness, and a decrease in the rate of decomposition of wood were observed.
32493269	9	63	theme	wood	1188:1191	arg1	rate					1207:1210	the wood decomposition rate	1184:1210	the wood decomposition rate of the transgenic aspen stems	1184:1240	Undescribed earlier considerable reduction in the wood decomposition rate of the transgenic aspen stems was also revealed for the transformed transgenic lines.
32493269	14	64	contain	carrying	1803:1810	arg2	gene					1835:1838	the recombinant sp-Xeg gene	1812:1838	the recombinant sp-Xeg gene	1812:1838	Our results showed that the plants carrying the recombinant sp-Xeg gene do not demonstrate a decrease in growth parameters in semi-natural conditions.
32493269	14	64	contain	carrying	1803:1810	arg1	plants					1796:1801	the plants	1792:1801	the plants carrying the recombinant sp-Xeg gene	1792:1838	Our results showed that the plants carrying the recombinant sp-Xeg gene do not demonstrate a decrease in growth parameters in semi-natural conditions.
32493269	6	65	theme	control	941:947	arg1	line					963:966	the control untransformed line	937:966	the control untransformed line	937:966	The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt.
32493269	15	66	theme	wood	2086:2089	arg1	decomposition					2069:2081	decomposition	2069:2081	decomposition of wood	2069:2089	In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness, and a decrease in the rate of decomposition of wood were observed.
32493269	4	67	contain	carrying	497:504	arg2	gene					536:539	the recombinant xyloglucanase gene	506:539	the recombinant xyloglucanase gene (sp-Xeg)	506:548	RESULTS Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time.
32493269	4	67	contain	carrying	497:504	arg2	sp-Xeg					542:547	sp-Xeg	542:547	sp-Xeg	542:547	RESULTS Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time.
32493269	4	67	contain	carrying	497:504	arg1	aspens					472:477	the transgenic aspens	457:477	the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions	457:602	RESULTS Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time.
32493269	4	68	dep	aspens	472:477	arg1	tremula					488:494	Populus tremula	480:494	Populus tremula	480:494	RESULTS Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time.
32493269	4	69	theme	semi-natural	580:591	arg1	conditions					593:602	semi-natural conditions	580:602	semi-natural conditions	580:602	RESULTS Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time.
32493269	11	70	theme	direct	1454:1459	arg1	dependence					1461:1470	A direct dependence	1452:1470	A direct dependence of the phenotypic and biochemical traits on the expression of the recombinant gene sp-Xeg	1452:1560	CONCLUSION A direct dependence of the phenotypic and biochemical traits on the expression of the recombinant gene sp-Xeg was demonstrated.
32493269	5	71	theme	carbohydrate	661:672	arg1	composition					674:684	carbohydrate composition	661:684	carbohydrate composition of wood	661:692	Change of carbohydrate composition of wood was observed in transgenic aspens carrying the sp-Xeg gene.
32493269	9	72	theme	considerable	1158:1169	arg1	reduction					1171:1179	Undescribed earlier considerable reduction	1138:1179	Undescribed earlier considerable reduction in the wood decomposition rate of the transgenic aspen stems	1138:1240	Undescribed earlier considerable reduction in the wood decomposition rate of the transgenic aspen stems was also revealed for the transformed transgenic lines.
32493269	9	73	theme	aspen	1230:1234	arg1	stems					1236:1240	the transgenic aspen stems	1215:1240	the transgenic aspen stems	1215:1240	Undescribed earlier considerable reduction in the wood decomposition rate of the transgenic aspen stems was also revealed for the transformed transgenic lines.
32493269	15	74	from	change	1947:1952	arg1	rate					2061:2064	the rate	2057:2064	the rate of decomposition of wood	2057:2089	In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness, and a decrease in the rate of decomposition of wood were observed.
32493269	15	74	from	change	1947:1952	arg1	composition					1974:1984	the carbohydrate composition	1957:1984	the carbohydrate composition of the wood	1957:1996	In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness, and a decrease in the rate of decomposition of wood were observed.
32493269	15	74	from	change	1947:1952	arg1	thickness					2028:2036	the cell wall thickness	2014:2036	the cell wall thickness	2014:2036	In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness, and a decrease in the rate of decomposition of wood were observed.
32493269	6	75	theme	accelerated	808:818	arg1	growth					820:825	accelerated growth	808:825	accelerated growth	808:825	The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt.
32493269	8	76	dep	lines	1131:1135	arg1	both					1109:1112	both	1109:1112	both	1109:1112	Thicker cell-wall and longer xylem fiber were also observed in both these transgenic lines.
32493269	1	77	theme	woody	212:216	arg1	plants					218:223	transgenic woody plants	201:223	transgenic woody plants with improved phenotypic traits	201:255	BACKGROUND Recombinant carbohydrases genes are used to produce transgenic woody plants with improved phenotypic traits.
32493269	12	78	theme	were	1656:1659	arg1	expression					1624:1633	the sp-Xeg gene expression	1608:1633	the sp-Xeg gene expression	1608:1633	The higher was the level of the sp-Xeg gene expression, the more pronounced were changes in the phenotypic and biochemical traits.
32493269	12	78	theme	were	1656:1659	arg1	changes					1661:1667	the more pronounced were changes	1636:1667	the more pronounced were changes in the phenotypic and biochemical traits	1636:1708	The higher was the level of the sp-Xeg gene expression, the more pronounced were changes in the phenotypic and biochemical traits.
32493269	6	79	theme	transformed	758:768	arg1	Xeg-2-1b					786:793	The transformed transgenic line Xeg-2-1b	754:793	The transformed transgenic line Xeg-2-1b	754:793	The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt.
32493269	0	80	from	canescens	77:85	arg1	transgenic					90:99	transgenic	90:99	transgenic	90:99	Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions.
32493269	0	80	from	canescens	77:85	arg1	effects					8:14	Various effects	0:14	Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic	0:99	Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions.
32493269	12	81	from	changes	1661:1667	arg1	traits					1703:1708	the phenotypic and biochemical traits	1672:1708	the phenotypic and biochemical traits	1672:1708	The higher was the level of the sp-Xeg gene expression, the more pronounced were changes in the phenotypic and biochemical traits.
32493269	15	82	theme	wood	1993:1996	arg1	composition					1974:1984	the carbohydrate composition	1957:1984	the carbohydrate composition of the wood	1957:1996	In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness, and a decrease in the rate of decomposition of wood were observed.
32493269	10	83	with	comparison	1390:1399	arg1	Pt					1437:1438	the control untransformed line Pt	1406:1438	the control untransformed line Pt	1406:1438	The decomposition rate was approximately twice as lower for the transgenic line Xeg-2-3b in comparison with the control untransformed line Pt.
32493269	12	84	theme	gene	1619:1622	arg1	expression					1624:1633	the sp-Xeg gene expression	1608:1633	the sp-Xeg gene expression	1608:1633	The higher was the level of the sp-Xeg gene expression, the more pronounced were changes in the phenotypic and biochemical traits.
32493269	12	84	theme	gene	1619:1622	arg1	changes					1661:1667	the more pronounced were changes	1636:1667	the more pronounced were changes in the phenotypic and biochemical traits	1636:1708	The higher was the level of the sp-Xeg gene expression, the more pronounced were changes in the phenotypic and biochemical traits.
32493269	4	85	theme	aspens	472:477	arg1	cultivation					442:452	successful cultivation	431:452	successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions	431:602	RESULTS Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time.
32493269	9	86	theme	transgenic	1280:1289	arg1	lines					1291:1295	the transformed transgenic lines	1264:1295	the transformed transgenic lines	1264:1295	Undescribed earlier considerable reduction in the wood decomposition rate of the transgenic aspen stems was also revealed for the transformed transgenic lines.
32493269	2	87	theme	such	282:285	arg1	plants					287:292	such plants	282:292	such plants	282:292	However, cultivation of such plants in open field is challenging due to a number of problems.
32493269	7	88	theme	line	1031:1034	arg1	Xeg-1-1c					1036:1043	the transgenic line Xeg-1-1c	1016:1043	the transgenic line Xeg-1-1c	1016:1043	The accelerated growth was observed also in the transgenic line Xeg-1-1c.
32493269	15	89	located	observed	2096:2103	arg2	change					1947:1952	a change	1945:1952	a change in the carbohydrate composition of the wood	1945:1996	In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness, and a decrease in the rate of decomposition of wood were observed.
32493269	15	89	located	observed	2096:2103	arg2	increase					2002:2009	an increase	1999:2009	an increase in the cell wall thickness	1999:2036	In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness, and a decrease in the rate of decomposition of wood were observed.
32493269	15	89	located	observed	2096:2103	arg1	lines					1938:1942	some transgenic lines	1922:1942	some transgenic lines	1922:1942	In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness, and a decrease in the rate of decomposition of wood were observed.
32493269	15	89	located	observed	2096:2103	arg2	decrease					2045:2052	a decrease	2043:2052	a decrease in the rate of decomposition of wood	2043:2089	In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness, and a decrease in the rate of decomposition of wood were observed.
32493269	4	90	theme	cultivation	442:452	arg1	Results					420:426	Results	420:426	Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions	420:602	RESULTS Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time.
32493269	15	91	from	increase	2002:2009	arg1	rate					2061:2064	the rate	2057:2064	the rate of decomposition of wood	2057:2089	In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness, and a decrease in the rate of decomposition of wood were observed.
32493269	15	91	from	increase	2002:2009	arg1	composition					1974:1984	the carbohydrate composition	1957:1984	the carbohydrate composition of the wood	1957:1996	In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness, and a decrease in the rate of decomposition of wood were observed.
32493269	15	91	from	increase	2002:2009	arg1	thickness					2028:2036	the cell wall thickness	2014:2036	the cell wall thickness	2014:2036	In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness, and a decrease in the rate of decomposition of wood were observed.
32493269	4	92	theme	first	639:643	arg1	time					645:648	the first time	635:648	the first time	635:648	RESULTS Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time.
32493269	1	93	theme	Recombinant	149:159	arg1	genes					175:179	BACKGROUND Recombinant carbohydrases genes	138:179	BACKGROUND Recombinant carbohydrases genes	138:179	BACKGROUND Recombinant carbohydrases genes are used to produce transgenic woody plants with improved phenotypic traits.
32493269	1	94	used	used	185:188	arg2	genes					175:179	BACKGROUND Recombinant carbohydrases genes	138:179	BACKGROUND Recombinant carbohydrases genes	138:179	BACKGROUND Recombinant carbohydrases genes are used to produce transgenic woody plants with improved phenotypic traits.
32493269	10	95	theme	decomposition	1302:1314	arg1	lower					1348:1352	lower	1348:1352	lower	1348:1352	The decomposition rate was approximately twice as lower for the transgenic line Xeg-2-3b in comparison with the control untransformed line Pt.
32493269	10	95	theme	decomposition	1302:1314	arg1	rate					1316:1319	The decomposition rate	1298:1319	The decomposition rate	1298:1319	The decomposition rate was approximately twice as lower for the transgenic line Xeg-2-3b in comparison with the control untransformed line Pt.
32493269	0	96	theme	semi-natural	113:124	arg1	conditions					126:135	semi-natural conditions	113:135	semi-natural conditions	113:135	Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions.
32493269	4	97	theme	xyloglucanase	522:534	arg1	sp-Xeg					542:547	sp-Xeg	542:547	sp-Xeg	542:547	RESULTS Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time.
32493269	4	97	theme	xyloglucanase	522:534	arg1	gene					536:539	the recombinant xyloglucanase gene	506:539	the recombinant xyloglucanase gene (sp-Xeg)	506:548	RESULTS Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time.
32493269	11	98	theme	gene	1550:1553	arg1	sp-Xeg					1555:1560	the recombinant gene sp-Xeg	1534:1560	the recombinant gene sp-Xeg	1534:1560	CONCLUSION A direct dependence of the phenotypic and biochemical traits on the expression of the recombinant gene sp-Xeg was demonstrated.
32493269	8	99	theme	longer	1068:1073	arg1	fiber					1081:1085	longer xylem fiber	1068:1085	longer xylem fiber	1068:1085	Thicker cell-wall and longer xylem fiber were also observed in both these transgenic lines.
32493269	3	100	theme	additional	363:372	arg1	research					374:381	additional research	363:381	additional research	363:381	Therefore, additional research is needed to alleviate them.
32493269	11	101	theme	phenotypic	1479:1488	arg1	traits					1506:1511	the phenotypic and biochemical traits	1475:1511	the phenotypic and biochemical traits	1475:1511	CONCLUSION A direct dependence of the phenotypic and biochemical traits on the expression of the recombinant gene sp-Xeg was demonstrated.
32493269	7	102	theme	accelerated	976:986	arg1	growth					988:993	The accelerated growth	972:993	The accelerated growth	972:993	The accelerated growth was observed also in the transgenic line Xeg-1-1c.
32493269	12	103	theme	expression	1624:1633	arg1	higher					1584:1589	higher	1584:1589	higher	1584:1589	The higher was the level of the sp-Xeg gene expression, the more pronounced were changes in the phenotypic and biochemical traits.
32493269	12	103	theme	expression	1624:1633	arg1	level					1599:1603	the level	1595:1603	the level of the sp-Xeg gene expression, the more pronounced were changes in the phenotypic and biochemical traits	1595:1708	The higher was the level of the sp-Xeg gene expression, the more pronounced were changes in the phenotypic and biochemical traits.
32493269	5	104	contain	carrying	728:735	arg1	aspens					721:726	transgenic aspens	710:726	transgenic aspens carrying the sp-Xeg gene	710:751	Change of carbohydrate composition of wood was observed in transgenic aspens carrying the sp-Xeg gene.
32493269	5	104	contain	carrying	728:735	arg2	gene					748:751	the sp-Xeg gene	737:751	the sp-Xeg gene	737:751	Change of carbohydrate composition of wood was observed in transgenic aspens carrying the sp-Xeg gene.
32493269	13	105	theme	phenotypic	1728:1737	arg1	changes					1739:1745	phenotypic changes	1728:1745	phenotypic changes in the leave traits	1728:1765	All lines showed phenotypic changes in the leave traits.
32493269	4	106	dep	RESULTS	412:418	arg1	reported					608:615	reported	608:615	are reported in this paper for the first time	604:648	RESULTS Results of successful cultivation of the transgenic aspens (Populus tremula) carrying the recombinant xyloglucanase gene (sp-Xeg) from Penicillium canescens in semi-natural conditions are reported in this paper for the first time.
32493269	11	107	theme	biochemical	1494:1504	arg1	traits					1506:1511	the phenotypic and biochemical traits	1475:1511	the phenotypic and biochemical traits	1475:1511	CONCLUSION A direct dependence of the phenotypic and biochemical traits on the expression of the recombinant gene sp-Xeg was demonstrated.
32493269	6	108	from	growth	820:825	arg1	wood					865:868	wood	865:868	wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt	865:969	The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt.
32493269	15	109	theme	wall	2023:2026	arg1	thickness					2028:2036	the cell wall thickness	2014:2036	the cell wall thickness	2014:2036	In some transgenic lines, a change in the carbohydrate composition of the wood, an increase in the cell wall thickness, and a decrease in the rate of decomposition of wood were observed.
32493269	6	110	theme	line	781:784	arg1	Xeg-2-1b					786:793	The transformed transgenic line Xeg-2-1b	754:793	The transformed transgenic line Xeg-2-1b	754:793	The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt.
32493269	0	111	theme	xyloglucanase	41:53	arg1	gene					55:58	the xyloglucanase gene	37:58	the xyloglucanase gene	37:58	Various effects of the expression of the xyloglucanase gene from Penicillium canescens in transgenic aspen under semi-natural conditions.
32493269	14	112	theme	recombinant	1816:1826	arg1	gene					1835:1838	the recombinant sp-Xeg gene	1812:1838	the recombinant sp-Xeg gene	1812:1838	Our results showed that the plants carrying the recombinant sp-Xeg gene do not demonstrate a decrease in growth parameters in semi-natural conditions.
32493269	6	113	theme	trees	873:877	arg1	wood					865:868	wood	865:868	wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt	865:969	The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt.
32493269	6	114	dep	both	890:893	arg1	greenhouse					895:904	greenhouse	895:904	greenhouse	895:904	The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt.
32493269	10	115	theme	line	1373:1376	arg1	Xeg-2-3b					1378:1385	the transgenic line Xeg-2-3b	1358:1385	the transgenic line Xeg-2-3b	1358:1385	The decomposition rate was approximately twice as lower for the transgenic line Xeg-2-3b in comparison with the control untransformed line Pt.
32493269	10	116	theme	control	1410:1416	arg1	Pt					1437:1438	the control untransformed line Pt	1406:1438	the control untransformed line Pt	1406:1438	The decomposition rate was approximately twice as lower for the transgenic line Xeg-2-3b in comparison with the control untransformed line Pt.
32493269	6	117	theme	cellulose	852:860	arg1	growth					820:825	accelerated growth	808:825	accelerated growth	808:825	The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt.
32493269	6	117	theme	cellulose	852:860	arg1	content					841:847	increased content	831:847	increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt	831:969	The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt.
32493269	9	118	dep	Undescribed	1138:1148	arg1	earlier					1150:1156	earlier	1150:1156	earlier	1150:1156	Undescribed earlier considerable reduction in the wood decomposition rate of the transgenic aspen stems was also revealed for the transformed transgenic lines.
32493269	10	119	theme	line	1432:1435	arg1	Pt					1437:1438	the control untransformed line Pt	1406:1438	the control untransformed line Pt	1406:1438	The decomposition rate was approximately twice as lower for the transgenic line Xeg-2-3b in comparison with the control untransformed line Pt.
32493269	6	120	from	comparison	921:930	arg1	outside					910:916	outside	910:916	outside	910:916	The transformed transgenic line Xeg-2-1b demonstrated accelerated growth and increased content of cellulose in wood of trees growing in both greenhouse and outside in comparison with the control untransformed line Pt.
32493269	9	121	theme	decomposition	1193:1205	arg1	rate					1207:1210	the wood decomposition rate	1184:1210	the wood decomposition rate of the transgenic aspen stems	1184:1240	Undescribed earlier considerable reduction in the wood decomposition rate of the transgenic aspen stems was also revealed for the transformed transgenic lines.
32493269	14	122	from	decrease	1861:1868	arg1	parameters					1880:1889	growth parameters	1873:1889	growth parameters in semi-natural conditions	1873:1916	Our results showed that the plants carrying the recombinant sp-Xeg gene do not demonstrate a decrease in growth parameters in semi-natural conditions.
32232517	8	0	theme	only	960:963	arg1	MK-7					947:950	MK-7	947:950	MK-7	947:950	The cell-wall sugars of SYSU K30005T were ribose, galactose and mannose and MK-7 was the only quinone.
32232517	8	0	theme	only	960:963	arg1	quinone					965:971	the only quinone	956:971	the only quinone	956:971	The cell-wall sugars of SYSU K30005T were ribose, galactose and mannose and MK-7 was the only quinone.
32232517	4	1	theme	phylogenetic	450:461	arg1	tree					463:466	phylogenetic tree	450:466	phylogenetic tree	450:466	In phylogenetic tree, strain SYSU K30005T clade with the members of the genus Lysinibacillus.
32232517	12	2	theme	nucleotide	1271:1280	arg1	values					1291:1296	The average nucleotide identity values	1259:1296	The average nucleotide identity values between SYSU K30005T and its closest relatives	1259:1343	The average nucleotide identity values between SYSU K30005T and its closest relatives were below the cut-off level (95-96%) for species delineation.
32232517	7	3	theme	Cell	823:826	arg1	type					847:850	Cell wall peptidoglycan type	823:850	Cell wall peptidoglycan type	823:850	Cell wall peptidoglycan type was A4α (Lys-Asp).
32232517	4	4	theme	Lysinibacillus	525:538	arg1	members					504:510	the members	500:510	the members of the genus Lysinibacillus	500:538	In phylogenetic tree, strain SYSU K30005T clade with the members of the genus Lysinibacillus.
32232517	3	5	theme	Strain	296:301	arg1	K30005T					308:314	Strain SYSU K30005T	296:314	Strain SYSU K30005T	296:314	Strain SYSU K30005T showed the highest 16S rRNA gene sequence similarity with Lysinibacillus fusiformis (98.6%) and Lysinibacillus sphaericus (98.2%).
32232517	2	6	theme	rod-shaped	93:102	arg1	bacterium					138:146	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium	59:146	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium	59:146	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium, designated strain SYSU K30005T, was isolated from a soil sample collected from a karst cave in Libo county, Guizhou province, south-western China.
32232517	2	6	theme	rod-shaped	93:102	arg1	strain					160:165	designated strain	149:165	designated strain SYSU K30005T	149:178	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium, designated strain SYSU K30005T, was isolated from a soil sample collected from a karst cave in Libo county, Guizhou province, south-western China.
32232517	5	7	theme	strain	597:602	arg1	K30005T					609:615	strain SYSU K30005T	597:615	strain SYSU K30005T	597:615	Based on the phylogenetic and 16S gene sequence result, strain SYSU K30005T was affiliated to the genus Lysinibacillus.
32232517	2	8	dep	strain	160:165	arg1	K30005T					172:178	K30005T	172:178	K30005T	172:178	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium, designated strain SYSU K30005T, was isolated from a soil sample collected from a karst cave in Libo county, Guizhou province, south-western China.
32232517	3	9	theme	highest	327:333	arg1	similarity					358:367	the highest 16S rRNA gene sequence similarity	323:367	the highest 16S rRNA gene sequence similarity with Lysinibacillus fusiformis (98.6%) and Lysinibacillus sphaericus (98.2%)	323:444	Strain SYSU K30005T showed the highest 16S rRNA gene sequence similarity with Lysinibacillus fusiformis (98.6%) and Lysinibacillus sphaericus (98.2%).
32232517	6	10	theme	%	786:786	arg1	NaCl					794:797	0-4% (w/v) NaCl	783:797	0-4% (w/v) NaCl (optimum in 3.5% NaCl)	783:820	The growth of SYSU K30005T was observed at 15-37 °C (optimum, 28 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-4% (w/v) NaCl (optimum in 3.5% NaCl).
32232517	5	11	theme	SYSU	604:607	arg1	K30005T					609:615	strain SYSU K30005T	597:615	strain SYSU K30005T	597:615	Based on the phylogenetic and 16S gene sequence result, strain SYSU K30005T was affiliated to the genus Lysinibacillus.
32232517	1	12	theme	cave	48:51	arg1	soil					53:56	cave soil	48:56	cave soil	48:56	nov., isolated from cave soil.
32232517	9	13	theme	fatty	978:982	arg1	iso-C15:0					1023:1031	iso-C15:0	1023:1031	iso-C15:0	1023:1031	The fatty acids (> 5% of total fatty acids) were iso-C15:0, anteiso-C15:0, iso-C16:0 and iso-C17:0.
32232517	9	13	theme	fatty	978:982	arg1	acids					984:988	The fatty acids	974:988	The fatty acids (> 5% of total fatty acids)	974:1016	The fatty acids (> 5% of total fatty acids) were iso-C15:0, anteiso-C15:0, iso-C16:0 and iso-C17:0.
32232517	7	14	theme	wall	828:831	arg1	type					847:850	Cell wall peptidoglycan type	823:850	Cell wall peptidoglycan type	823:850	Cell wall peptidoglycan type was A4α (Lys-Asp).
32232517	3	15	theme	16S	335:337	arg1	similarity					358:367	the highest 16S rRNA gene sequence similarity	323:367	the highest 16S rRNA gene sequence similarity with Lysinibacillus fusiformis (98.6%) and Lysinibacillus sphaericus (98.2%)	323:444	Strain SYSU K30005T showed the highest 16S rRNA gene sequence similarity with Lysinibacillus fusiformis (98.6%) and Lysinibacillus sphaericus (98.2%).
32232517	2	16	theme	designated	149:158	arg1	bacterium					138:146	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium	59:146	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium	59:146	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium, designated strain SYSU K30005T, was isolated from a soil sample collected from a karst cave in Libo county, Guizhou province, south-western China.
32232517	2	16	theme	designated	149:158	arg1	strain					160:165	designated strain	149:165	designated strain SYSU K30005T	149:178	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium, designated strain SYSU K30005T, was isolated from a soil sample collected from a karst cave in Libo county, Guizhou province, south-western China.
32232517	5	17	theme	phylogenetic	554:565	arg1	result					589:594	the phylogenetic and 16S gene sequence result	550:594	the phylogenetic and 16S gene sequence result	550:594	Based on the phylogenetic and 16S gene sequence result, strain SYSU K30005T was affiliated to the genus Lysinibacillus.
32232517	11	18	theme	genomic	1218:1224	arg1	content					1236:1242	The genomic DNA G + C content	1214:1242	The genomic DNA G + C content	1214:1242	The genomic DNA G + C content was 37.2 mol%.
32232517	11	18	theme	genomic	1218:1224	arg1	%					1256:1256	37.2 mol%	1248:1256	37.2 mol%	1248:1256	The genomic DNA G + C content was 37.2 mol%.
32232517	6	19	dep	optimum	714:720	arg1	28 °C					723:727	28 °C	723:727	28 °C	723:727	The growth of SYSU K30005T was observed at 15-37 °C (optimum, 28 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-4% (w/v) NaCl (optimum in 3.5% NaCl).
32232517	15	20	theme	SYSU	1607:1610	arg1	1.17492T					1643:1650	= KCTC 43130T = CGMCC 1.17492T	1621:1650	= KCTC 43130T = CGMCC 1.17492T	1621:1650	The type strain is SYSU K30005T (= KCTC 43130T = CGMCC 1.17492T).
32232517	15	20	theme	SYSU	1607:1610	arg1	strain					1597:1602	The type strain	1588:1602	The type strain	1588:1602	The type strain is SYSU K30005T (= KCTC 43130T = CGMCC 1.17492T).
32232517	15	20	theme	SYSU	1607:1610	arg1	K30005T					1612:1618	SYSU K30005T	1607:1618	SYSU K30005T (= KCTC 43130T = CGMCC 1.17492T)	1607:1651	The type strain is SYSU K30005T (= KCTC 43130T = CGMCC 1.17492T).
32232517	11	21	theme	G + C	1230:1234	arg1	content					1236:1242	The genomic DNA G + C content	1214:1242	The genomic DNA G + C content	1214:1242	The genomic DNA G + C content was 37.2 mol%.
32232517	11	21	theme	G + C	1230:1234	arg1	%					1256:1256	37.2 mol%	1248:1256	37.2 mol%	1248:1256	The genomic DNA G + C content was 37.2 mol%.
32232517	10	22	theme	polar	1078:1082	arg1	profile					1091:1097	The polar lipids profile	1074:1097	The polar lipids profile	1074:1097	The polar lipids profile included diphosphatidylglycerol, phosphatideylglycerol, phosphatidylethanolamine and an unidentified phospholipid.
32232517	5	23	theme	16S	571:573	arg1	result					589:594	the phylogenetic and 16S gene sequence result	550:594	the phylogenetic and 16S gene sequence result	550:594	Based on the phylogenetic and 16S gene sequence result, strain SYSU K30005T was affiliated to the genus Lysinibacillus.
32232517	15	24	theme	43130T = CGMCC	1628:1641	arg1	1.17492T					1643:1650	= KCTC 43130T = CGMCC 1.17492T	1621:1650	= KCTC 43130T = CGMCC 1.17492T	1621:1650	The type strain is SYSU K30005T (= KCTC 43130T = CGMCC 1.17492T).
32232517	15	24	theme	43130T = CGMCC	1628:1641	arg1	K30005T					1612:1618	SYSU K30005T	1607:1618	SYSU K30005T (= KCTC 43130T = CGMCC 1.17492T)	1607:1651	The type strain is SYSU K30005T (= KCTC 43130T = CGMCC 1.17492T).
32232517	6	25	located	observed	692:699	arg2	growth					665:670	The growth	661:670	The growth of SYSU K30005T	661:686	The growth of SYSU K30005T was observed at 15-37 °C (optimum, 28 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-4% (w/v) NaCl (optimum in 3.5% NaCl).
32232517	6	25	located	observed	692:699	arg1	pH					731:732	pH 6.0-9.0	731:740	pH 6.0-9.0 (optimum, pH 7.0)	731:758	The growth of SYSU K30005T was observed at 15-37 °C (optimum, 28 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-4% (w/v) NaCl (optimum in 3.5% NaCl).
32232517	6	25	located	observed	692:699	arg1	optimum					714:720	optimum	714:720	optimum	714:720	The growth of SYSU K30005T was observed at 15-37 °C (optimum, 28 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-4% (w/v) NaCl (optimum in 3.5% NaCl).
32232517	6	25	located	observed	692:699	arg1	15-37 °C					704:711	15-37 °C	704:711	15-37 °C (optimum, 28 °C)	704:728	The growth of SYSU K30005T was observed at 15-37 °C (optimum, 28 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-4% (w/v) NaCl (optimum in 3.5% NaCl).
32232517	12	26	theme	average	1263:1269	arg1	values					1291:1296	The average nucleotide identity values	1259:1296	The average nucleotide identity values between SYSU K30005T and its closest relatives	1259:1343	The average nucleotide identity values between SYSU K30005T and its closest relatives were below the cut-off level (95-96%) for species delineation.
32232517	5	27	theme	gene	575:578	arg1	result					589:594	the phylogenetic and 16S gene sequence result	550:594	the phylogenetic and 16S gene sequence result	550:594	Based on the phylogenetic and 16S gene sequence result, strain SYSU K30005T was affiliated to the genus Lysinibacillus.
32232517	12	28	theme	cut-off	1360:1366	arg1	level					1368:1372	the cut-off level	1356:1372	the cut-off level (95-96%)	1356:1381	The average nucleotide identity values between SYSU K30005T and its closest relatives were below the cut-off level (95-96%) for species delineation.
32232517	12	28	theme	cut-off	1360:1366	arg1	%					1380:1380	95-96%	1375:1380	95-96%	1375:1380	The average nucleotide identity values between SYSU K30005T and its closest relatives were below the cut-off level (95-96%) for species delineation.
32232517	15	29	theme	= KCTC	1621:1626	arg1	1.17492T					1643:1650	= KCTC 43130T = CGMCC 1.17492T	1621:1650	= KCTC 43130T = CGMCC 1.17492T	1621:1650	The type strain is SYSU K30005T (= KCTC 43130T = CGMCC 1.17492T).
32232517	15	29	theme	= KCTC	1621:1626	arg1	K30005T					1612:1618	SYSU K30005T	1607:1618	SYSU K30005T (= KCTC 43130T = CGMCC 1.17492T)	1607:1651	The type strain is SYSU K30005T (= KCTC 43130T = CGMCC 1.17492T).
32232517	0	30	theme	cavernae	15:22	arg1	sp					24:25	Lysinibacillus cavernae sp	0:25	Lysinibacillus cavernae sp.	0:26	Lysinibacillus cavernae sp.
32232517	2	31	theme	endospore-forming	120:136	arg1	bacterium					138:146	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium	59:146	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium	59:146	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium, designated strain SYSU K30005T, was isolated from a soil sample collected from a karst cave in Libo county, Guizhou province, south-western China.
32232517	2	31	theme	endospore-forming	120:136	arg1	strain					160:165	designated strain	149:165	designated strain SYSU K30005T	149:178	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium, designated strain SYSU K30005T, was isolated from a soil sample collected from a karst cave in Libo county, Guizhou province, south-western China.
32232517	5	32	theme	sequence	580:587	arg1	result					589:594	the phylogenetic and 16S gene sequence result	550:594	the phylogenetic and 16S gene sequence result	550:594	Based on the phylogenetic and 16S gene sequence result, strain SYSU K30005T was affiliated to the genus Lysinibacillus.
32232517	9	33	theme	total	999:1003	arg1	acids					1011:1015	total fatty acids	999:1015	total fatty acids	999:1015	The fatty acids (> 5% of total fatty acids) were iso-C15:0, anteiso-C15:0, iso-C16:0 and iso-C17:0.
32232517	13	34	theme	Lysinibacillus	1555:1568	arg1	sp					1579:1580	the name Lysinibacillus cavernae sp	1546:1580	the name Lysinibacillus cavernae sp	1546:1580	The results support the conclusion that strain SYSU K30005T represents a novel species of the genus Lysinibacillus, for which we proposed the name Lysinibacillus cavernae sp.
32232517	13	35	theme	strain	1448:1453	arg1	K30005T					1460:1466	strain SYSU K30005T	1448:1466	strain SYSU K30005T	1448:1466	The results support the conclusion that strain SYSU K30005T represents a novel species of the genus Lysinibacillus, for which we proposed the name Lysinibacillus cavernae sp.
32232517	0	36	theme	Lysinibacillus	0:13	arg1	sp					24:25	Lysinibacillus cavernae sp	0:25	Lysinibacillus cavernae sp.	0:26	Lysinibacillus cavernae sp.
32232517	6	37	dep	optimum	743:749	arg1	pH					752:753	pH 7.0	752:757	pH 7.0	752:757	The growth of SYSU K30005T was observed at 15-37 °C (optimum, 28 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-4% (w/v) NaCl (optimum in 3.5% NaCl).
32232517	13	38	theme	Lysinibacillus	1508:1521	arg1	species					1487:1493	a novel species	1479:1493	a novel species	1479:1493	The results support the conclusion that strain SYSU K30005T represents a novel species of the genus Lysinibacillus, for which we proposed the name Lysinibacillus cavernae sp.
32232517	9	39	theme	fatty	1005:1009	arg1	acids					1011:1015	total fatty acids	999:1015	total fatty acids	999:1015	The fatty acids (> 5% of total fatty acids) were iso-C15:0, anteiso-C15:0, iso-C16:0 and iso-C17:0.
32232517	2	40	theme	south-western	275:287	arg1	county					249:254	county	249:254	county	249:254	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium, designated strain SYSU K30005T, was isolated from a soil sample collected from a karst cave in Libo county, Guizhou province, south-western China.
32232517	2	40	theme	south-western	275:287	arg1	China					289:293	south-western China	275:293	south-western China	275:293	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium, designated strain SYSU K30005T, was isolated from a soil sample collected from a karst cave in Libo county, Guizhou province, south-western China.
32232517	15	41	theme	type	1592:1595	arg1	strain					1597:1602	The type strain	1588:1602	The type strain	1588:1602	The type strain is SYSU K30005T (= KCTC 43130T = CGMCC 1.17492T).
32232517	15	41	theme	type	1592:1595	arg1	K30005T					1612:1618	SYSU K30005T	1607:1618	SYSU K30005T (= KCTC 43130T = CGMCC 1.17492T)	1607:1651	The type strain is SYSU K30005T (= KCTC 43130T = CGMCC 1.17492T).
32232517	2	42	attach	isolated	185:192	arg2	strain					160:165	designated strain	149:165	designated strain SYSU K30005T	149:178	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium, designated strain SYSU K30005T, was isolated from a soil sample collected from a karst cave in Libo county, Guizhou province, south-western China.
32232517	2	42	attach	isolated	185:192	arg2	bacterium					138:146	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium	59:146	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium	59:146	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium, designated strain SYSU K30005T, was isolated from a soil sample collected from a karst cave in Libo county, Guizhou province, south-western China.
32232517	2	42	attach	isolated	185:192	arg1	sample					206:211	a soil sample	199:211	a soil sample collected from a karst cave in Libo county, Guizhou province, south-western China	199:293	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium, designated strain SYSU K30005T, was isolated from a soil sample collected from a karst cave in Libo county, Guizhou province, south-western China.
32232517	3	43	theme	rRNA	339:342	arg1	similarity					358:367	the highest 16S rRNA gene sequence similarity	323:367	the highest 16S rRNA gene sequence similarity with Lysinibacillus fusiformis (98.6%) and Lysinibacillus sphaericus (98.2%)	323:444	Strain SYSU K30005T showed the highest 16S rRNA gene sequence similarity with Lysinibacillus fusiformis (98.6%) and Lysinibacillus sphaericus (98.2%).
32232517	5	44	theme	genus	639:643	arg1	Lysinibacillus					645:658	the genus Lysinibacillus	635:658	the genus Lysinibacillus	635:658	Based on the phylogenetic and 16S gene sequence result, strain SYSU K30005T was affiliated to the genus Lysinibacillus.
32232517	9	45	theme	acids	1011:1015	arg1	%					994:994	> 5%	991:994	> 5% of total fatty acids	991:1015	The fatty acids (> 5% of total fatty acids) were iso-C15:0, anteiso-C15:0, iso-C16:0 and iso-C17:0.
32232517	9	45	theme	acids	1011:1015	arg1	acids					1011:1015	total fatty acids	999:1015	total fatty acids	999:1015	The fatty acids (> 5% of total fatty acids) were iso-C15:0, anteiso-C15:0, iso-C16:0 and iso-C17:0.
32232517	3	46	theme	gene	344:347	arg1	similarity					358:367	the highest 16S rRNA gene sequence similarity	323:367	the highest 16S rRNA gene sequence similarity with Lysinibacillus fusiformis (98.6%) and Lysinibacillus sphaericus (98.2%)	323:444	Strain SYSU K30005T showed the highest 16S rRNA gene sequence similarity with Lysinibacillus fusiformis (98.6%) and Lysinibacillus sphaericus (98.2%).
32232517	6	47	theme	%	814:814	arg1	NaCl					816:819	3.5% NaCl	811:819	3.5% NaCl	811:819	The growth of SYSU K30005T was observed at 15-37 °C (optimum, 28 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-4% (w/v) NaCl (optimum in 3.5% NaCl).
32232517	8	48	theme	K30005T	900:906	arg1	ribose					913:918	ribose	913:918	ribose	913:918	The cell-wall sugars of SYSU K30005T were ribose, galactose and mannose and MK-7 was the only quinone.
32232517	8	48	theme	K30005T	900:906	arg1	sugars					885:890	The cell-wall sugars	871:890	The cell-wall sugars of SYSU K30005T	871:906	The cell-wall sugars of SYSU K30005T were ribose, galactose and mannose and MK-7 was the only quinone.
32232517	3	49	with	similarity	358:367	arg1	sphaericus					427:436	Lysinibacillus sphaericus	412:436	Lysinibacillus sphaericus (98.2%)	412:444	Strain SYSU K30005T showed the highest 16S rRNA gene sequence similarity with Lysinibacillus fusiformis (98.6%) and Lysinibacillus sphaericus (98.2%).
32232517	3	49	with	similarity	358:367	arg1	%					443:443	98.2%	439:443	98.2%	439:443	Strain SYSU K30005T showed the highest 16S rRNA gene sequence similarity with Lysinibacillus fusiformis (98.6%) and Lysinibacillus sphaericus (98.2%).
32232517	3	49	with	similarity	358:367	arg1	fusiformis					389:398	Lysinibacillus fusiformis	374:398	Lysinibacillus fusiformis (98.6%)	374:406	Strain SYSU K30005T showed the highest 16S rRNA gene sequence similarity with Lysinibacillus fusiformis (98.6%) and Lysinibacillus sphaericus (98.2%).
32232517	3	49	with	similarity	358:367	arg1	%					405:405	98.6%	401:405	98.6%	401:405	Strain SYSU K30005T showed the highest 16S rRNA gene sequence similarity with Lysinibacillus fusiformis (98.6%) and Lysinibacillus sphaericus (98.2%).
32232517	6	50	theme	w/v	789:791	arg1	NaCl					794:797	0-4% (w/v) NaCl	783:797	0-4% (w/v) NaCl (optimum in 3.5% NaCl)	783:820	The growth of SYSU K30005T was observed at 15-37 °C (optimum, 28 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-4% (w/v) NaCl (optimum in 3.5% NaCl).
32232517	6	51	from	NaCl	816:819	arg1	optimum					800:806	optimum	800:806	optimum	800:806	The growth of SYSU K30005T was observed at 15-37 °C (optimum, 28 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-4% (w/v) NaCl (optimum in 3.5% NaCl).
32232517	6	52	theme	3.5	811:813	arg1	%					814:814	%	814:814	%	814:814	The growth of SYSU K30005T was observed at 15-37 °C (optimum, 28 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-4% (w/v) NaCl (optimum in 3.5% NaCl).
32232517	13	53	theme	novel	1481:1485	arg1	species					1487:1493	a novel species	1479:1493	a novel species	1479:1493	The results support the conclusion that strain SYSU K30005T represents a novel species of the genus Lysinibacillus, for which we proposed the name Lysinibacillus cavernae sp.
32232517	6	54	dep	NaCl	794:797	arg1	optimum					800:806	optimum	800:806	optimum	800:806	The growth of SYSU K30005T was observed at 15-37 °C (optimum, 28 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-4% (w/v) NaCl (optimum in 3.5% NaCl).
32232517	2	55	from	cave	236:239	arg1	county					249:254	county	249:254	county	249:254	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium, designated strain SYSU K30005T, was isolated from a soil sample collected from a karst cave in Libo county, Guizhou province, south-western China.
32232517	2	55	from	cave	236:239	arg1	province					265:272	province	265:272	province	265:272	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium, designated strain SYSU K30005T, was isolated from a soil sample collected from a karst cave in Libo county, Guizhou province, south-western China.
32232517	2	55	from	cave	236:239	arg1	China					289:293	south-western China	275:293	south-western China	275:293	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium, designated strain SYSU K30005T, was isolated from a soil sample collected from a karst cave in Libo county, Guizhou province, south-western China.
32232517	4	56	from	clade	489:493	arg1	tree					463:466	phylogenetic tree	450:466	phylogenetic tree	450:466	In phylogenetic tree, strain SYSU K30005T clade with the members of the genus Lysinibacillus.
32232517	2	57	theme	motile	85:90	arg1	bacterium					138:146	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium	59:146	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium	59:146	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium, designated strain SYSU K30005T, was isolated from a soil sample collected from a karst cave in Libo county, Guizhou province, south-western China.
32232517	2	57	theme	motile	85:90	arg1	strain					160:165	designated strain	149:165	designated strain SYSU K30005T	149:178	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium, designated strain SYSU K30005T, was isolated from a soil sample collected from a karst cave in Libo county, Guizhou province, south-western China.
32232517	4	58	theme	genus	519:523	arg1	Lysinibacillus					525:538	the genus Lysinibacillus	515:538	the genus Lysinibacillus	515:538	In phylogenetic tree, strain SYSU K30005T clade with the members of the genus Lysinibacillus.
32232517	6	59	theme	NaCl	794:797	arg1	presence					771:778	the presence	767:778	the presence of 0-4% (w/v) NaCl (optimum in 3.5% NaCl)	767:820	The growth of SYSU K30005T was observed at 15-37 °C (optimum, 28 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-4% (w/v) NaCl (optimum in 3.5% NaCl).
32232517	13	60	theme	name	1550:1553	arg1	sp					1579:1580	the name Lysinibacillus cavernae sp	1546:1580	the name Lysinibacillus cavernae sp	1546:1580	The results support the conclusion that strain SYSU K30005T represents a novel species of the genus Lysinibacillus, for which we proposed the name Lysinibacillus cavernae sp.
32232517	12	61	theme	species	1387:1393	arg1	delineation					1395:1405	species delineation	1387:1405	species delineation	1387:1405	The average nucleotide identity values between SYSU K30005T and its closest relatives were below the cut-off level (95-96%) for species delineation.
32232517	10	62	theme	lipids	1084:1089	arg1	profile					1091:1097	The polar lipids profile	1074:1097	The polar lipids profile	1074:1097	The polar lipids profile included diphosphatidylglycerol, phosphatideylglycerol, phosphatidylethanolamine and an unidentified phospholipid.
32232517	3	63	theme	Lysinibacillus	374:387	arg1	fusiformis					389:398	Lysinibacillus fusiformis	374:398	Lysinibacillus fusiformis (98.6%)	374:406	Strain SYSU K30005T showed the highest 16S rRNA gene sequence similarity with Lysinibacillus fusiformis (98.6%) and Lysinibacillus sphaericus (98.2%).
32232517	3	63	theme	Lysinibacillus	374:387	arg1	%					405:405	98.6%	401:405	98.6%	401:405	Strain SYSU K30005T showed the highest 16S rRNA gene sequence similarity with Lysinibacillus fusiformis (98.6%) and Lysinibacillus sphaericus (98.2%).
32232517	2	64	theme	positive	75:82	arg1	bacterium					138:146	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium	59:146	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium	59:146	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium, designated strain SYSU K30005T, was isolated from a soil sample collected from a karst cave in Libo county, Guizhou province, south-western China.
32232517	2	64	theme	positive	75:82	arg1	strain					160:165	designated strain	149:165	designated strain SYSU K30005T	149:178	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium, designated strain SYSU K30005T, was isolated from a soil sample collected from a karst cave in Libo county, Guizhou province, south-western China.
32232517	12	65	theme	identity	1282:1289	arg1	values					1291:1296	The average nucleotide identity values	1259:1296	The average nucleotide identity values between SYSU K30005T and its closest relatives	1259:1343	The average nucleotide identity values between SYSU K30005T and its closest relatives were below the cut-off level (95-96%) for species delineation.
32232517	2	66	theme	soil	201:204	arg1	sample					206:211	a soil sample	199:211	a soil sample collected from a karst cave in Libo county, Guizhou province, south-western China	199:293	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium, designated strain SYSU K30005T, was isolated from a soil sample collected from a karst cave in Libo county, Guizhou province, south-western China.
32232517	8	67	theme	cell-wall	875:883	arg1	ribose					913:918	ribose	913:918	ribose	913:918	The cell-wall sugars of SYSU K30005T were ribose, galactose and mannose and MK-7 was the only quinone.
32232517	8	67	theme	cell-wall	875:883	arg1	sugars					885:890	The cell-wall sugars	871:890	The cell-wall sugars of SYSU K30005T	871:906	The cell-wall sugars of SYSU K30005T were ribose, galactose and mannose and MK-7 was the only quinone.
32232517	12	68	theme	closest	1327:1333	arg1	relatives					1335:1343	its closest relatives	1323:1343	its closest relatives	1323:1343	The average nucleotide identity values between SYSU K30005T and its closest relatives were below the cut-off level (95-96%) for species delineation.
32232517	2	69	theme	Gram-staining	61:73	arg1	bacterium					138:146	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium	59:146	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium	59:146	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium, designated strain SYSU K30005T, was isolated from a soil sample collected from a karst cave in Libo county, Guizhou province, south-western China.
32232517	2	69	theme	Gram-staining	61:73	arg1	strain					160:165	designated strain	149:165	designated strain SYSU K30005T	149:178	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium, designated strain SYSU K30005T, was isolated from a soil sample collected from a karst cave in Libo county, Guizhou province, south-western China.
32232517	13	70	theme	SYSU	1455:1458	arg1	K30005T					1460:1466	strain SYSU K30005T	1448:1466	strain SYSU K30005T	1448:1466	The results support the conclusion that strain SYSU K30005T represents a novel species of the genus Lysinibacillus, for which we proposed the name Lysinibacillus cavernae sp.
32232517	6	71	from	optimum	800:806	arg1	NaCl					816:819	3.5% NaCl	811:819	3.5% NaCl	811:819	The growth of SYSU K30005T was observed at 15-37 °C (optimum, 28 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-4% (w/v) NaCl (optimum in 3.5% NaCl).
32232517	13	72	theme	genus	1502:1506	arg1	Lysinibacillus					1508:1521	the genus Lysinibacillus	1498:1521	the genus Lysinibacillus	1498:1521	The results support the conclusion that strain SYSU K30005T represents a novel species of the genus Lysinibacillus, for which we proposed the name Lysinibacillus cavernae sp.
32232517	6	73	theme	K30005T	680:686	arg1	growth					665:670	The growth	661:670	The growth of SYSU K30005T	661:686	The growth of SYSU K30005T was observed at 15-37 °C (optimum, 28 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-4% (w/v) NaCl (optimum in 3.5% NaCl).
32232517	9	74	dep	acids	984:988	arg1	%					994:994	> 5%	991:994	> 5% of total fatty acids	991:1015	The fatty acids (> 5% of total fatty acids) were iso-C15:0, anteiso-C15:0, iso-C16:0 and iso-C17:0.
32232517	9	74	dep	acids	984:988	arg1	acids					1011:1015	total fatty acids	999:1015	total fatty acids	999:1015	The fatty acids (> 5% of total fatty acids) were iso-C15:0, anteiso-C15:0, iso-C16:0 and iso-C17:0.
32232517	13	75	dep	Lysinibacillus	1555:1568	arg1	cavernae					1570:1577	cavernae	1570:1577	cavernae	1570:1577	The results support the conclusion that strain SYSU K30005T represents a novel species of the genus Lysinibacillus, for which we proposed the name Lysinibacillus cavernae sp.
32232517	3	76	theme	SYSU	303:306	arg1	K30005T					308:314	Strain SYSU K30005T	296:314	Strain SYSU K30005T	296:314	Strain SYSU K30005T showed the highest 16S rRNA gene sequence similarity with Lysinibacillus fusiformis (98.6%) and Lysinibacillus sphaericus (98.2%).
32232517	10	77	theme	unidentified	1187:1198	arg1	phospholipid					1200:1211	an unidentified phospholipid	1184:1211	an unidentified phospholipid	1184:1211	The polar lipids profile included diphosphatidylglycerol, phosphatideylglycerol, phosphatidylethanolamine and an unidentified phospholipid.
32232517	2	78	theme	karst	230:234	arg1	cave					236:239	a karst cave	228:239	a karst cave in Libo county, Guizhou province, south-western China	228:293	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium, designated strain SYSU K30005T, was isolated from a soil sample collected from a karst cave in Libo county, Guizhou province, south-western China.
32232517	7	79	theme	peptidoglycan	833:845	arg1	type					847:850	Cell wall peptidoglycan type	823:850	Cell wall peptidoglycan type	823:850	Cell wall peptidoglycan type was A4α (Lys-Asp).
32232517	11	80	theme	DNA	1226:1228	arg1	content					1236:1242	The genomic DNA G + C content	1214:1242	The genomic DNA G + C content	1214:1242	The genomic DNA G + C content was 37.2 mol%.
32232517	11	80	theme	DNA	1226:1228	arg1	%					1256:1256	37.2 mol%	1248:1256	37.2 mol%	1248:1256	The genomic DNA G + C content was 37.2 mol%.
32232517	2	81	theme	subterminal	108:118	arg1	bacterium					138:146	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium	59:146	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium	59:146	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium, designated strain SYSU K30005T, was isolated from a soil sample collected from a karst cave in Libo county, Guizhou province, south-western China.
32232517	2	81	theme	subterminal	108:118	arg1	strain					160:165	designated strain	149:165	designated strain SYSU K30005T	149:178	A Gram-staining positive, motile, rod-shaped and subterminal endospore-forming bacterium, designated strain SYSU K30005T, was isolated from a soil sample collected from a karst cave in Libo county, Guizhou province, south-western China.
32232517	4	82	with	clade	489:493	arg1	members					504:510	the members	500:510	the members of the genus Lysinibacillus	500:538	In phylogenetic tree, strain SYSU K30005T clade with the members of the genus Lysinibacillus.
32232517	3	83	theme	sequence	349:356	arg1	similarity					358:367	the highest 16S rRNA gene sequence similarity	323:367	the highest 16S rRNA gene sequence similarity with Lysinibacillus fusiformis (98.6%) and Lysinibacillus sphaericus (98.2%)	323:444	Strain SYSU K30005T showed the highest 16S rRNA gene sequence similarity with Lysinibacillus fusiformis (98.6%) and Lysinibacillus sphaericus (98.2%).
32232517	11	84	theme	37.2 mol	1248:1255	arg1	content					1236:1242	The genomic DNA G + C content	1214:1242	The genomic DNA G + C content	1214:1242	The genomic DNA G + C content was 37.2 mol%.
32232517	11	84	theme	37.2 mol	1248:1255	arg1	%					1256:1256	37.2 mol%	1248:1256	37.2 mol%	1248:1256	The genomic DNA G + C content was 37.2 mol%.
34225242	8	0	used	used	1165:1168	arg2	methodology					1115:1125	Response surface methodology	1098:1125	Response surface methodology based on central composite design	1098:1159	Response surface methodology based on central composite design was used to optimize the extraction procedure including pH, amount of adsorbent, extraction time, desorption time, and volume of elution solvent.
34225242	13	1	theme	single	2039:2044	arg1	dose					2066:2069	a single oral or intravenous dose	2037:2069	a single oral or intravenous dose	2037:2069	The method was applied to study the pharmacokinetics, metabolism, and bioavailability of trans-resveratrol in healthy rats following a single oral or intravenous dose.
34225242	7	2	theme	chromatography	1039:1052	arg1	spectrometry					1084:1095	ultra-performance liquid chromatography triple-quadrupole tandem mass spectrometry	1014:1095	ultra-performance liquid chromatography triple-quadrupole tandem mass spectrometry	1014:1095	A qualitative analysis was performed using ultra-performance liquid chromatography triple-quadrupole tandem mass spectrometry.
34225242	0	3	from	plasma	191:196	arg1	metabolites					170:180	its metabolites	166:180	its metabolites from rat plasma	166:196	Construction of Fe3O4/SiO2/chitosan-grafted-poly(N-vinylcaprolactam) magnetic nanocomposite and their application in simultaneous extraction of Trans-resveratrol and its metabolites from rat plasma.
34225242	0	3	from	plasma	191:196	arg1	Construction					0:11	Construction	0:11	Construction of Fe3O4/SiO2/chitosan-grafted-poly(N-vinylcaprolactam) magnetic nanocomposite and their application in simultaneous extraction of Trans-resveratrol	0:160	Construction of Fe3O4/SiO2/chitosan-grafted-poly(N-vinylcaprolactam) magnetic nanocomposite and their application in simultaneous extraction of Trans-resveratrol and its metabolites from rat plasma.
34225242	13	4	theme	trans-resveratrol	1993:2009	arg1	pharmacokinetics					1940:1955	pharmacokinetics	1940:1955	pharmacokinetics	1940:1955	The method was applied to study the pharmacokinetics, metabolism, and bioavailability of trans-resveratrol in healthy rats following a single oral or intravenous dose.
34225242	13	4	theme	trans-resveratrol	1993:2009	arg1	metabolism					1958:1967	metabolism	1958:1967	metabolism	1958:1967	The method was applied to study the pharmacokinetics, metabolism, and bioavailability of trans-resveratrol in healthy rats following a single oral or intravenous dose.
34225242	13	4	theme	trans-resveratrol	1993:2009	arg1	bioavailability					1974:1988	bioavailability	1974:1988	bioavailability	1974:1988	The method was applied to study the pharmacokinetics, metabolism, and bioavailability of trans-resveratrol in healthy rats following a single oral or intravenous dose.
34225242	6	5	theme	rat	959:961	arg1	plasma					963:968	rat plasma	959:968	rat plasma	959:968	The application of Fe3O4/SiO2/CHT-g-PNVCL MNC was utilized as a novel adsorbent for the simultaneous extraction of trans-resveratrol and its major phase II metabolites from rat plasma.
34225242	7	6	theme	ultra-performance	1014:1030	arg1	spectrometry					1084:1095	ultra-performance liquid chromatography triple-quadrupole tandem mass spectrometry	1014:1095	ultra-performance liquid chromatography triple-quadrupole tandem mass spectrometry	1014:1095	A qualitative analysis was performed using ultra-performance liquid chromatography triple-quadrupole tandem mass spectrometry.
34225242	13	7	theme	healthy	2014:2020	arg1	rats					2022:2025	healthy rats	2014:2025	healthy rats	2014:2025	The method was applied to study the pharmacokinetics, metabolism, and bioavailability of trans-resveratrol in healthy rats following a single oral or intravenous dose.
34225242	1	8	theme	magnetic	207:214	arg1	nanocomposite					216:228	A novel magnetic nanocomposite	199:228	A novel magnetic nanocomposite of chitosan-grafted-poly(N-vinylcaprolactam) (Fe3O4/SiO2/CHT-g-PNVCL MNC)	199:302	A novel magnetic nanocomposite of chitosan-grafted-poly(N-vinylcaprolactam) (Fe3O4/SiO2/CHT-g-PNVCL MNC) were synthesized.
34225242	1	8	theme	magnetic	207:214	arg1	chitosan-grafted-poly					233:253	chitosan-grafted-poly	233:253	chitosan-grafted-poly(N-vinylcaprolactam) (Fe3O4/SiO2/CHT-g-PNVCL MNC)	233:302	A novel magnetic nanocomposite of chitosan-grafted-poly(N-vinylcaprolactam) (Fe3O4/SiO2/CHT-g-PNVCL MNC) were synthesized.
34225242	5	9	theme	nanomaterials	771:783	arg1	properties					745:754	physicochemical properties	729:754	physicochemical properties of synthesized nanomaterials	729:783	Various techniques were used to characterize of physicochemical properties of synthesized nanomaterials.
34225242	6	10	theme	phase	933:937	arg1	metabolites					942:952	its major phase II metabolites	923:952	its major phase II metabolites from rat plasma	923:968	The application of Fe3O4/SiO2/CHT-g-PNVCL MNC was utilized as a novel adsorbent for the simultaneous extraction of trans-resveratrol and its major phase II metabolites from rat plasma.
34225242	2	11	theme	shells	356:361	arg1	monodon					371:377	shrimp shells Penaeus monodon	349:377	shrimp shells Penaeus monodon	349:377	Chitosan was prepared from shrimp shells Penaeus monodon by a green deacetylation approach.
34225242	13	12	theme	oral	2046:2049	arg1	dose					2066:2069	a single oral or intravenous dose	2037:2069	a single oral or intravenous dose	2037:2069	The method was applied to study the pharmacokinetics, metabolism, and bioavailability of trans-resveratrol in healthy rats following a single oral or intravenous dose.
34225242	0	13	from	application	102:112	arg1	extraction					130:139	simultaneous extraction	117:139	simultaneous extraction of Trans-resveratrol	117:160	Construction of Fe3O4/SiO2/chitosan-grafted-poly(N-vinylcaprolactam) magnetic nanocomposite and their application in simultaneous extraction of Trans-resveratrol and its metabolites from rat plasma.
34225242	10	14	dep	6.9	1510:1512	arg1	to					1507:1508	to	1507:1508	to	1507:1508	The results of the validation of the method indicated its acceptable accuracy (-4.4 to 6.9%), linearity (r > 0.995), precision (CV < 6.3%), and stability.
34225242	0	15	theme	rat	187:189	arg1	plasma					191:196	rat plasma	187:196	rat plasma	187:196	Construction of Fe3O4/SiO2/chitosan-grafted-poly(N-vinylcaprolactam) magnetic nanocomposite and their application in simultaneous extraction of Trans-resveratrol and its metabolites from rat plasma.
34225242	10	16	theme	validation	1442:1451	arg1	results					1427:1433	The results	1423:1433	The results of the validation of the method	1423:1465	The results of the validation of the method indicated its acceptable accuracy (-4.4 to 6.9%), linearity (r > 0.995), precision (CV < 6.3%), and stability.
34225242	11	17	theme	target	1670:1675	arg1	limits					1588:1593	The lower limits	1578:1593	The lower limits of quantification of the proposed method achieved	1578:1643	The lower limits of quantification of the proposed method achieved were 1.23-1.68 ngmL-1for target analytes.
34225242	11	17	theme	target	1670:1675	arg1	analytes					1677:1684	1.23-1.68 ngmL-1for target analytes	1650:1684	1.23-1.68 ngmL-1for target analytes	1650:1684	The lower limits of quantification of the proposed method achieved were 1.23-1.68 ngmL-1for target analytes.
34225242	13	18	from	pharmacokinetics	1940:1955	arg1	rats					2022:2025	healthy rats	2014:2025	healthy rats	2014:2025	The method was applied to study the pharmacokinetics, metabolism, and bioavailability of trans-resveratrol in healthy rats following a single oral or intravenous dose.
34225242	12	19	used	used	1748:1751	arg2	information					1691:1701	The information	1687:1701	The information obtained from the method validation	1687:1737	The information obtained from the method validation has been used to estimate the expanded uncertainty for the determination of trans-resveratrol in rat plasma samples following orally administered trans-resveratrol.
34225242	0	20	theme	application	102:112	arg1	metabolites					170:180	its metabolites	166:180	its metabolites from rat plasma	166:196	Construction of Fe3O4/SiO2/chitosan-grafted-poly(N-vinylcaprolactam) magnetic nanocomposite and their application in simultaneous extraction of Trans-resveratrol and its metabolites from rat plasma.
34225242	0	20	theme	application	102:112	arg1	Construction					0:11	Construction	0:11	Construction of Fe3O4/SiO2/chitosan-grafted-poly(N-vinylcaprolactam) magnetic nanocomposite and their application in simultaneous extraction of Trans-resveratrol	0:160	Construction of Fe3O4/SiO2/chitosan-grafted-poly(N-vinylcaprolactam) magnetic nanocomposite and their application in simultaneous extraction of Trans-resveratrol and its metabolites from rat plasma.
34225242	0	21	from	Construction	0:11	arg1	extraction					130:139	simultaneous extraction	117:139	simultaneous extraction of Trans-resveratrol	117:160	Construction of Fe3O4/SiO2/chitosan-grafted-poly(N-vinylcaprolactam) magnetic nanocomposite and their application in simultaneous extraction of Trans-resveratrol and its metabolites from rat plasma.
34225242	9	22	theme	biological	1394:1403	arg1	methods					1414:1420	biological analysis methods	1394:1420	biological analysis methods	1394:1420	The established quantitative method succeeded in satisfying FDA requirements regarding biological analysis methods.
34225242	0	23	theme	simultaneous	117:128	arg1	extraction					130:139	simultaneous extraction	117:139	simultaneous extraction of Trans-resveratrol	117:160	Construction of Fe3O4/SiO2/chitosan-grafted-poly(N-vinylcaprolactam) magnetic nanocomposite and their application in simultaneous extraction of Trans-resveratrol and its metabolites from rat plasma.
34225242	8	24	theme	adsorbent	1231:1239	arg1	amount					1221:1226	amount	1221:1226	amount of adsorbent	1221:1239	Response surface methodology based on central composite design was used to optimize the extraction procedure including pH, amount of adsorbent, extraction time, desorption time, and volume of elution solvent.
34225242	8	24	theme	adsorbent	1231:1239	arg1	solvent					1298:1304	elution solvent	1290:1304	elution solvent	1290:1304	Response surface methodology based on central composite design was used to optimize the extraction procedure including pH, amount of adsorbent, extraction time, desorption time, and volume of elution solvent.
34225242	8	24	theme	adsorbent	1231:1239	arg1	adsorbent					1231:1239	adsorbent	1231:1239	adsorbent	1231:1239	Response surface methodology based on central composite design was used to optimize the extraction procedure including pH, amount of adsorbent, extraction time, desorption time, and volume of elution solvent.
34225242	8	24	theme	adsorbent	1231:1239	arg1	time					1270:1273	desorption time	1259:1273	desorption time	1259:1273	Response surface methodology based on central composite design was used to optimize the extraction procedure including pH, amount of adsorbent, extraction time, desorption time, and volume of elution solvent.
34225242	8	24	theme	adsorbent	1231:1239	arg1	volume					1280:1285	volume	1280:1285	volume of elution solvent	1280:1304	Response surface methodology based on central composite design was used to optimize the extraction procedure including pH, amount of adsorbent, extraction time, desorption time, and volume of elution solvent.
34225242	8	24	theme	adsorbent	1231:1239	arg1	pH					1217:1218	pH	1217:1218	pH	1217:1218	Response surface methodology based on central composite design was used to optimize the extraction procedure including pH, amount of adsorbent, extraction time, desorption time, and volume of elution solvent.
34225242	8	24	theme	adsorbent	1231:1239	arg1	time					1253:1256	extraction time	1242:1256	extraction time	1242:1256	Response surface methodology based on central composite design was used to optimize the extraction procedure including pH, amount of adsorbent, extraction time, desorption time, and volume of elution solvent.
34225242	12	25	theme	rat	1836:1838	arg1	samples					1847:1853	rat plasma samples	1836:1853	rat plasma samples following orally administered trans-resveratrol	1836:1901	The information obtained from the method validation has been used to estimate the expanded uncertainty for the determination of trans-resveratrol in rat plasma samples following orally administered trans-resveratrol.
34225242	5	26	used	used	705:708	arg2	techniques					689:698	Various techniques	681:698	Various techniques	681:698	Various techniques were used to characterize of physicochemical properties of synthesized nanomaterials.
34225242	11	27	theme	quantification	1598:1611	arg1	limits					1588:1593	The lower limits	1578:1593	The lower limits of quantification of the proposed method achieved	1578:1643	The lower limits of quantification of the proposed method achieved were 1.23-1.68 ngmL-1for target analytes.
34225242	11	27	theme	quantification	1598:1611	arg1	analytes					1677:1684	1.23-1.68 ngmL-1for target analytes	1650:1684	1.23-1.68 ngmL-1for target analytes	1650:1684	The lower limits of quantification of the proposed method achieved were 1.23-1.68 ngmL-1for target analytes.
34225242	3	28	theme	atom	526:529	arg1	transfer					531:538	surface-initiated atom transfer radical polymerization	508:561	surface-initiated atom transfer radical polymerization	508:561	N-vinylcaprolactam was first polymerized on the surface of Fe3O4 magnetic nanoparticles using surface-initiated atom transfer radical polymerization.
34225242	13	29	from	metabolism	1958:1967	arg1	rats					2022:2025	healthy rats	2014:2025	healthy rats	2014:2025	The method was applied to study the pharmacokinetics, metabolism, and bioavailability of trans-resveratrol in healthy rats following a single oral or intravenous dose.
34225242	8	30	theme	extraction	1242:1251	arg1	time					1253:1256	extraction time	1242:1256	extraction time	1242:1256	Response surface methodology based on central composite design was used to optimize the extraction procedure including pH, amount of adsorbent, extraction time, desorption time, and volume of elution solvent.
34225242	5	31	theme	Various	681:687	arg1	techniques					689:698	Various techniques	681:698	Various techniques	681:698	Various techniques were used to characterize of physicochemical properties of synthesized nanomaterials.
34225242	6	32	used	utilized	836:843	arg2	adsorbent					856:864	adsorbent	856:864	adsorbent	856:864	The application of Fe3O4/SiO2/CHT-g-PNVCL MNC was utilized as a novel adsorbent for the simultaneous extraction of trans-resveratrol and its major phase II metabolites from rat plasma.
34225242	6	32	used	utilized	836:843	arg2	application					790:800	The application	786:800	The application of Fe3O4/SiO2/CHT-g-PNVCL MNC	786:830	The application of Fe3O4/SiO2/CHT-g-PNVCL MNC was utilized as a novel adsorbent for the simultaneous extraction of trans-resveratrol and its major phase II metabolites from rat plasma.
34225242	4	33	theme	Fe3O4	574:578	arg1	nanoparticles					580:592	the Fe3O4 nanoparticles	570:592	the Fe3O4 nanoparticles modified with carboxyl-terminated- poly(N-vinylcaprolactam)	570:652	Then, the Fe3O4 nanoparticles modified with carboxyl-terminated- poly(N-vinylcaprolactam) was grafted onto chitosan.
34225242	10	34	theme	acceptable	1481:1490	arg1	%					1513:1513	-4.4 to 6.9%	1502:1513	-4.4 to 6.9%	1502:1513	The results of the validation of the method indicated its acceptable accuracy (-4.4 to 6.9%), linearity (r > 0.995), precision (CV < 6.3%), and stability.
34225242	10	34	theme	acceptable	1481:1490	arg1	accuracy					1492:1499	its acceptable accuracy	1477:1499	its acceptable accuracy (-4.4 to 6.9%)	1477:1514	The results of the validation of the method indicated its acceptable accuracy (-4.4 to 6.9%), linearity (r > 0.995), precision (CV < 6.3%), and stability.
34225242	12	35	theme	trans-resveratrol	1815:1831	arg1	determination					1798:1810	the determination	1794:1810	the determination of trans-resveratrol	1794:1831	The information obtained from the method validation has been used to estimate the expanded uncertainty for the determination of trans-resveratrol in rat plasma samples following orally administered trans-resveratrol.
34225242	6	36	from	plasma	963:968	arg1	extraction					887:896	the simultaneous extraction	870:896	the simultaneous extraction of trans-resveratrol	870:917	The application of Fe3O4/SiO2/CHT-g-PNVCL MNC was utilized as a novel adsorbent for the simultaneous extraction of trans-resveratrol and its major phase II metabolites from rat plasma.
34225242	6	36	from	plasma	963:968	arg1	metabolites					942:952	its major phase II metabolites	923:952	its major phase II metabolites from rat plasma	923:968	The application of Fe3O4/SiO2/CHT-g-PNVCL MNC was utilized as a novel adsorbent for the simultaneous extraction of trans-resveratrol and its major phase II metabolites from rat plasma.
34225242	11	37	theme	method	1629:1634	arg1	quantification					1598:1611	quantification	1598:1611	quantification of the proposed method achieved	1598:1643	The lower limits of quantification of the proposed method achieved were 1.23-1.68 ngmL-1for target analytes.
34225242	0	38	theme	Fe3O4/SiO2/chitosan-grafted-poly	16:47	arg1	nanocomposite					78:90	Fe3O4/SiO2/chitosan-grafted-poly(N-vinylcaprolactam) magnetic nanocomposite	16:90	Fe3O4/SiO2/chitosan-grafted-poly(N-vinylcaprolactam) magnetic nanocomposite	16:90	Construction of Fe3O4/SiO2/chitosan-grafted-poly(N-vinylcaprolactam) magnetic nanocomposite and their application in simultaneous extraction of Trans-resveratrol and its metabolites from rat plasma.
34225242	8	39	theme	surface	1107:1113	arg1	methodology					1115:1125	Response surface methodology	1098:1125	Response surface methodology based on central composite design	1098:1159	Response surface methodology based on central composite design was used to optimize the extraction procedure including pH, amount of adsorbent, extraction time, desorption time, and volume of elution solvent.
34225242	3	40	theme	magnetic	479:486	arg1	nanoparticles					488:500	Fe3O4 magnetic nanoparticles	473:500	Fe3O4 magnetic nanoparticles using surface-initiated atom transfer radical polymerization	473:561	N-vinylcaprolactam was first polymerized on the surface of Fe3O4 magnetic nanoparticles using surface-initiated atom transfer radical polymerization.
34225242	6	41	theme	Fe3O4/SiO2/CHT-g-PNVCL	805:826	arg1	MNC					828:830	Fe3O4/SiO2/CHT-g-PNVCL MNC	805:830	Fe3O4/SiO2/CHT-g-PNVCL MNC	805:830	The application of Fe3O4/SiO2/CHT-g-PNVCL MNC was utilized as a novel adsorbent for the simultaneous extraction of trans-resveratrol and its major phase II metabolites from rat plasma.
34225242	3	42	theme	radical	540:546	arg1	polymerization					548:561	radical polymerization	540:561	surface-initiated atom transfer radical polymerization	508:561	N-vinylcaprolactam was first polymerized on the surface of Fe3O4 magnetic nanoparticles using surface-initiated atom transfer radical polymerization.
34225242	0	43	theme	N-vinylcaprolactam	49:66	arg1	nanocomposite					78:90	Fe3O4/SiO2/chitosan-grafted-poly(N-vinylcaprolactam) magnetic nanocomposite	16:90	Fe3O4/SiO2/chitosan-grafted-poly(N-vinylcaprolactam) magnetic nanocomposite	16:90	Construction of Fe3O4/SiO2/chitosan-grafted-poly(N-vinylcaprolactam) magnetic nanocomposite and their application in simultaneous extraction of Trans-resveratrol and its metabolites from rat plasma.
34225242	12	44	from	uncertainty	1778:1788	arg1	samples					1847:1853	rat plasma samples	1836:1853	rat plasma samples following orally administered trans-resveratrol	1836:1901	The information obtained from the method validation has been used to estimate the expanded uncertainty for the determination of trans-resveratrol in rat plasma samples following orally administered trans-resveratrol.
34225242	9	45	theme	established	1311:1321	arg1	method					1336:1341	The established quantitative method	1307:1341	The established quantitative method	1307:1341	The established quantitative method succeeded in satisfying FDA requirements regarding biological analysis methods.
34225242	0	46	theme	magnetic	69:76	arg1	nanocomposite					78:90	Fe3O4/SiO2/chitosan-grafted-poly(N-vinylcaprolactam) magnetic nanocomposite	16:90	Fe3O4/SiO2/chitosan-grafted-poly(N-vinylcaprolactam) magnetic nanocomposite	16:90	Construction of Fe3O4/SiO2/chitosan-grafted-poly(N-vinylcaprolactam) magnetic nanocomposite and their application in simultaneous extraction of Trans-resveratrol and its metabolites from rat plasma.
34225242	3	47	dep	transfer	531:538	arg1	polymerization					548:561	radical polymerization	540:561	surface-initiated atom transfer radical polymerization	508:561	N-vinylcaprolactam was first polymerized on the surface of Fe3O4 magnetic nanoparticles using surface-initiated atom transfer radical polymerization.
34225242	8	48	theme	central	1136:1142	arg1	design					1154:1159	central composite design	1136:1159	central composite design	1136:1159	Response surface methodology based on central composite design was used to optimize the extraction procedure including pH, amount of adsorbent, extraction time, desorption time, and volume of elution solvent.
34225242	2	49	theme	green	384:388	arg1	approach					404:411	a green deacetylation approach	382:411	a green deacetylation approach	382:411	Chitosan was prepared from shrimp shells Penaeus monodon by a green deacetylation approach.
34225242	8	50	theme	solvent	1298:1304	arg1	amount					1221:1226	amount	1221:1226	amount of adsorbent	1221:1239	Response surface methodology based on central composite design was used to optimize the extraction procedure including pH, amount of adsorbent, extraction time, desorption time, and volume of elution solvent.
34225242	8	50	theme	solvent	1298:1304	arg1	solvent					1298:1304	elution solvent	1290:1304	elution solvent	1290:1304	Response surface methodology based on central composite design was used to optimize the extraction procedure including pH, amount of adsorbent, extraction time, desorption time, and volume of elution solvent.
34225242	8	50	theme	solvent	1298:1304	arg1	adsorbent					1231:1239	adsorbent	1231:1239	adsorbent	1231:1239	Response surface methodology based on central composite design was used to optimize the extraction procedure including pH, amount of adsorbent, extraction time, desorption time, and volume of elution solvent.
34225242	8	50	theme	solvent	1298:1304	arg1	time					1270:1273	desorption time	1259:1273	desorption time	1259:1273	Response surface methodology based on central composite design was used to optimize the extraction procedure including pH, amount of adsorbent, extraction time, desorption time, and volume of elution solvent.
34225242	8	50	theme	solvent	1298:1304	arg1	volume					1280:1285	volume	1280:1285	volume of elution solvent	1280:1304	Response surface methodology based on central composite design was used to optimize the extraction procedure including pH, amount of adsorbent, extraction time, desorption time, and volume of elution solvent.
34225242	8	50	theme	solvent	1298:1304	arg1	pH					1217:1218	pH	1217:1218	pH	1217:1218	Response surface methodology based on central composite design was used to optimize the extraction procedure including pH, amount of adsorbent, extraction time, desorption time, and volume of elution solvent.
34225242	8	50	theme	solvent	1298:1304	arg1	time					1253:1256	extraction time	1242:1256	extraction time	1242:1256	Response surface methodology based on central composite design was used to optimize the extraction procedure including pH, amount of adsorbent, extraction time, desorption time, and volume of elution solvent.
34225242	0	51	from	metabolites	170:180	arg1	extraction					130:139	simultaneous extraction	117:139	simultaneous extraction of Trans-resveratrol	117:160	Construction of Fe3O4/SiO2/chitosan-grafted-poly(N-vinylcaprolactam) magnetic nanocomposite and their application in simultaneous extraction of Trans-resveratrol and its metabolites from rat plasma.
34225242	6	52	theme	simultaneous	874:885	arg1	extraction					887:896	the simultaneous extraction	870:896	the simultaneous extraction of trans-resveratrol	870:917	The application of Fe3O4/SiO2/CHT-g-PNVCL MNC was utilized as a novel adsorbent for the simultaneous extraction of trans-resveratrol and its major phase II metabolites from rat plasma.
34225242	9	53	theme	FDA	1367:1369	arg1	requirements					1371:1382	FDA requirements	1367:1382	FDA requirements regarding biological analysis methods	1367:1420	The established quantitative method succeeded in satisfying FDA requirements regarding biological analysis methods.
34225242	7	54	theme	mass	1079:1082	arg1	spectrometry					1084:1095	ultra-performance liquid chromatography triple-quadrupole tandem mass spectrometry	1014:1095	ultra-performance liquid chromatography triple-quadrupole tandem mass spectrometry	1014:1095	A qualitative analysis was performed using ultra-performance liquid chromatography triple-quadrupole tandem mass spectrometry.
34225242	13	55	theme	intravenous	2054:2064	arg1	dose					2066:2069	a single oral or intravenous dose	2037:2069	a single oral or intravenous dose	2037:2069	The method was applied to study the pharmacokinetics, metabolism, and bioavailability of trans-resveratrol in healthy rats following a single oral or intravenous dose.
34225242	2	56	theme	shrimp	349:354	arg1	monodon					371:377	shrimp shells Penaeus monodon	349:377	shrimp shells Penaeus monodon	349:377	Chitosan was prepared from shrimp shells Penaeus monodon by a green deacetylation approach.
34225242	7	57	theme	triple-quadrupole	1054:1070	arg1	spectrometry					1084:1095	ultra-performance liquid chromatography triple-quadrupole tandem mass spectrometry	1014:1095	ultra-performance liquid chromatography triple-quadrupole tandem mass spectrometry	1014:1095	A qualitative analysis was performed using ultra-performance liquid chromatography triple-quadrupole tandem mass spectrometry.
34225242	8	58	theme	extraction	1186:1195	arg1	procedure					1197:1205	the extraction procedure	1182:1205	the extraction procedure including pH, amount of adsorbent, extraction time, desorption time, and volume of elution solvent	1182:1304	Response surface methodology based on central composite design was used to optimize the extraction procedure including pH, amount of adsorbent, extraction time, desorption time, and volume of elution solvent.
34225242	9	59	theme	analysis	1405:1412	arg1	methods					1414:1420	biological analysis methods	1394:1420	biological analysis methods	1394:1420	The established quantitative method succeeded in satisfying FDA requirements regarding biological analysis methods.
34225242	7	60	theme	liquid	1032:1037	arg1	spectrometry					1084:1095	ultra-performance liquid chromatography triple-quadrupole tandem mass spectrometry	1014:1095	ultra-performance liquid chromatography triple-quadrupole tandem mass spectrometry	1014:1095	A qualitative analysis was performed using ultra-performance liquid chromatography triple-quadrupole tandem mass spectrometry.
34225242	1	61	theme	novel	201:205	arg1	nanocomposite					216:228	A novel magnetic nanocomposite	199:228	A novel magnetic nanocomposite of chitosan-grafted-poly(N-vinylcaprolactam) (Fe3O4/SiO2/CHT-g-PNVCL MNC)	199:302	A novel magnetic nanocomposite of chitosan-grafted-poly(N-vinylcaprolactam) (Fe3O4/SiO2/CHT-g-PNVCL MNC) were synthesized.
34225242	1	61	theme	novel	201:205	arg1	chitosan-grafted-poly					233:253	chitosan-grafted-poly	233:253	chitosan-grafted-poly(N-vinylcaprolactam) (Fe3O4/SiO2/CHT-g-PNVCL MNC)	233:302	A novel magnetic nanocomposite of chitosan-grafted-poly(N-vinylcaprolactam) (Fe3O4/SiO2/CHT-g-PNVCL MNC) were synthesized.
34225242	5	62	theme	synthesized	759:769	arg1	nanomaterials					771:783	synthesized nanomaterials	759:783	synthesized nanomaterials	759:783	Various techniques were used to characterize of physicochemical properties of synthesized nanomaterials.
34225242	6	63	theme	II	939:940	arg1	metabolites					942:952	its major phase II metabolites	923:952	its major phase II metabolites from rat plasma	923:968	The application of Fe3O4/SiO2/CHT-g-PNVCL MNC was utilized as a novel adsorbent for the simultaneous extraction of trans-resveratrol and its major phase II metabolites from rat plasma.
34225242	6	64	theme	major	927:931	arg1	metabolites					942:952	its major phase II metabolites	923:952	its major phase II metabolites from rat plasma	923:968	The application of Fe3O4/SiO2/CHT-g-PNVCL MNC was utilized as a novel adsorbent for the simultaneous extraction of trans-resveratrol and its major phase II metabolites from rat plasma.
34225242	2	65	theme	Penaeus	363:369	arg1	monodon					371:377	shrimp shells Penaeus monodon	349:377	shrimp shells Penaeus monodon	349:377	Chitosan was prepared from shrimp shells Penaeus monodon by a green deacetylation approach.
34225242	1	66	theme	chitosan-grafted-poly	233:253	arg1	nanocomposite					216:228	A novel magnetic nanocomposite	199:228	A novel magnetic nanocomposite of chitosan-grafted-poly(N-vinylcaprolactam) (Fe3O4/SiO2/CHT-g-PNVCL MNC)	199:302	A novel magnetic nanocomposite of chitosan-grafted-poly(N-vinylcaprolactam) (Fe3O4/SiO2/CHT-g-PNVCL MNC) were synthesized.
34225242	1	66	theme	chitosan-grafted-poly	233:253	arg1	chitosan-grafted-poly					233:253	chitosan-grafted-poly	233:253	chitosan-grafted-poly(N-vinylcaprolactam) (Fe3O4/SiO2/CHT-g-PNVCL MNC)	233:302	A novel magnetic nanocomposite of chitosan-grafted-poly(N-vinylcaprolactam) (Fe3O4/SiO2/CHT-g-PNVCL MNC) were synthesized.
34225242	9	67	theme	quantitative	1323:1334	arg1	method					1336:1341	The established quantitative method	1307:1341	The established quantitative method	1307:1341	The established quantitative method succeeded in satisfying FDA requirements regarding biological analysis methods.
34225242	7	68	theme	qualitative	973:983	arg1	analysis					985:992	A qualitative analysis	971:992	A qualitative analysis	971:992	A qualitative analysis was performed using ultra-performance liquid chromatography triple-quadrupole tandem mass spectrometry.
34225242	4	69	theme	carboxyl-terminated-	608:627	arg1	N-vinylcaprolactam					634:651	N-vinylcaprolactam	634:651	N-vinylcaprolactam	634:651	Then, the Fe3O4 nanoparticles modified with carboxyl-terminated- poly(N-vinylcaprolactam) was grafted onto chitosan.
34225242	4	69	theme	carboxyl-terminated-	608:627	arg1	poly					629:632	carboxyl-terminated- poly	608:632	carboxyl-terminated- poly(N-vinylcaprolactam)	608:652	Then, the Fe3O4 nanoparticles modified with carboxyl-terminated- poly(N-vinylcaprolactam) was grafted onto chitosan.
34225242	12	70	theme	expanded	1769:1776	arg1	uncertainty					1778:1788	the expanded uncertainty	1765:1788	the expanded uncertainty for the determination of trans-resveratrol in rat plasma samples following orally administered trans-resveratrol	1765:1901	The information obtained from the method validation has been used to estimate the expanded uncertainty for the determination of trans-resveratrol in rat plasma samples following orally administered trans-resveratrol.
34225242	11	71	theme	1.23-1.68 ngmL-1for	1650:1668	arg1	limits					1588:1593	The lower limits	1578:1593	The lower limits of quantification of the proposed method achieved	1578:1643	The lower limits of quantification of the proposed method achieved were 1.23-1.68 ngmL-1for target analytes.
34225242	11	71	theme	1.23-1.68 ngmL-1for	1650:1668	arg1	analytes					1677:1684	1.23-1.68 ngmL-1for target analytes	1650:1684	1.23-1.68 ngmL-1for target analytes	1650:1684	The lower limits of quantification of the proposed method achieved were 1.23-1.68 ngmL-1for target analytes.
34225242	0	72	from	extraction	130:139	arg1	metabolites					170:180	its metabolites	166:180	its metabolites from rat plasma	166:196	Construction of Fe3O4/SiO2/chitosan-grafted-poly(N-vinylcaprolactam) magnetic nanocomposite and their application in simultaneous extraction of Trans-resveratrol and its metabolites from rat plasma.
34225242	0	72	from	extraction	130:139	arg1	Construction					0:11	Construction	0:11	Construction of Fe3O4/SiO2/chitosan-grafted-poly(N-vinylcaprolactam) magnetic nanocomposite and their application in simultaneous extraction of Trans-resveratrol	0:160	Construction of Fe3O4/SiO2/chitosan-grafted-poly(N-vinylcaprolactam) magnetic nanocomposite and their application in simultaneous extraction of Trans-resveratrol and its metabolites from rat plasma.
34225242	12	73	theme	administered	1872:1883	arg1	trans-resveratrol					1885:1901	orally administered trans-resveratrol	1865:1901	orally administered trans-resveratrol	1865:1901	The information obtained from the method validation has been used to estimate the expanded uncertainty for the determination of trans-resveratrol in rat plasma samples following orally administered trans-resveratrol.
34225242	10	74	theme	method	1460:1465	arg1	validation					1442:1451	the validation	1438:1451	the validation of the method	1438:1465	The results of the validation of the method indicated its acceptable accuracy (-4.4 to 6.9%), linearity (r > 0.995), precision (CV < 6.3%), and stability.
34225242	12	75	theme	method	1721:1726	arg1	validation					1728:1737	the method validation	1717:1737	the method validation	1717:1737	The information obtained from the method validation has been used to estimate the expanded uncertainty for the determination of trans-resveratrol in rat plasma samples following orally administered trans-resveratrol.
34225242	0	76	from	nanocomposite	78:90	arg1	extraction					130:139	simultaneous extraction	117:139	simultaneous extraction of Trans-resveratrol	117:160	Construction of Fe3O4/SiO2/chitosan-grafted-poly(N-vinylcaprolactam) magnetic nanocomposite and their application in simultaneous extraction of Trans-resveratrol and its metabolites from rat plasma.
34225242	0	77	theme	Trans-resveratrol	144:160	arg1	extraction					130:139	simultaneous extraction	117:139	simultaneous extraction of Trans-resveratrol	117:160	Construction of Fe3O4/SiO2/chitosan-grafted-poly(N-vinylcaprolactam) magnetic nanocomposite and their application in simultaneous extraction of Trans-resveratrol and its metabolites from rat plasma.
34225242	13	78	from	bioavailability	1974:1988	arg1	rats					2022:2025	healthy rats	2014:2025	healthy rats	2014:2025	The method was applied to study the pharmacokinetics, metabolism, and bioavailability of trans-resveratrol in healthy rats following a single oral or intravenous dose.
34225242	13	79	dep	pharmacokinetics	1940:1955	arg1	the					1936:1938	the	1936:1938	the	1936:1938	The method was applied to study the pharmacokinetics, metabolism, and bioavailability of trans-resveratrol in healthy rats following a single oral or intravenous dose.
34225242	5	80	theme	physicochemical	729:743	arg1	properties					745:754	physicochemical properties	729:754	physicochemical properties of synthesized nanomaterials	729:783	Various techniques were used to characterize of physicochemical properties of synthesized nanomaterials.
34225242	12	81	theme	plasma	1840:1845	arg1	samples					1847:1853	rat plasma samples	1836:1853	rat plasma samples following orally administered trans-resveratrol	1836:1901	The information obtained from the method validation has been used to estimate the expanded uncertainty for the determination of trans-resveratrol in rat plasma samples following orally administered trans-resveratrol.
34225242	11	82	theme	lower	1582:1586	arg1	limits					1588:1593	The lower limits	1578:1593	The lower limits of quantification of the proposed method achieved	1578:1643	The lower limits of quantification of the proposed method achieved were 1.23-1.68 ngmL-1for target analytes.
34225242	11	82	theme	lower	1582:1586	arg1	analytes					1677:1684	1.23-1.68 ngmL-1for target analytes	1650:1684	1.23-1.68 ngmL-1for target analytes	1650:1684	The lower limits of quantification of the proposed method achieved were 1.23-1.68 ngmL-1for target analytes.
34225242	8	83	theme	desorption	1259:1268	arg1	time					1270:1273	desorption time	1259:1273	desorption time	1259:1273	Response surface methodology based on central composite design was used to optimize the extraction procedure including pH, amount of adsorbent, extraction time, desorption time, and volume of elution solvent.
34225242	11	84	theme	proposed	1620:1627	arg1	method					1629:1634	the proposed method	1616:1634	the proposed method achieved	1616:1643	The lower limits of quantification of the proposed method achieved were 1.23-1.68 ngmL-1for target analytes.
34225242	3	85	theme	surface-initiated	508:524	arg1	transfer					531:538	surface-initiated atom transfer radical polymerization	508:561	surface-initiated atom transfer radical polymerization	508:561	N-vinylcaprolactam was first polymerized on the surface of Fe3O4 magnetic nanoparticles using surface-initiated atom transfer radical polymerization.
34225242	6	86	theme	MNC	828:830	arg1	application					790:800	The application	786:800	The application of Fe3O4/SiO2/CHT-g-PNVCL MNC	786:830	The application of Fe3O4/SiO2/CHT-g-PNVCL MNC was utilized as a novel adsorbent for the simultaneous extraction of trans-resveratrol and its major phase II metabolites from rat plasma.
34225242	6	86	theme	MNC	828:830	arg1	adsorbent					856:864	adsorbent	856:864	adsorbent	856:864	The application of Fe3O4/SiO2/CHT-g-PNVCL MNC was utilized as a novel adsorbent for the simultaneous extraction of trans-resveratrol and its major phase II metabolites from rat plasma.
34225242	8	87	theme	Response	1098:1105	arg1	methodology					1115:1125	Response surface methodology	1098:1125	Response surface methodology based on central composite design	1098:1159	Response surface methodology based on central composite design was used to optimize the extraction procedure including pH, amount of adsorbent, extraction time, desorption time, and volume of elution solvent.
34225242	3	88	theme	nanoparticles	488:500	arg1	surface					462:468	the surface	458:468	the surface of Fe3O4 magnetic nanoparticles using surface-initiated atom transfer radical polymerization	458:561	N-vinylcaprolactam was first polymerized on the surface of Fe3O4 magnetic nanoparticles using surface-initiated atom transfer radical polymerization.
34225242	0	89	theme	nanocomposite	78:90	arg1	metabolites					170:180	its metabolites	166:180	its metabolites from rat plasma	166:196	Construction of Fe3O4/SiO2/chitosan-grafted-poly(N-vinylcaprolactam) magnetic nanocomposite and their application in simultaneous extraction of Trans-resveratrol and its metabolites from rat plasma.
34225242	0	89	theme	nanocomposite	78:90	arg1	Construction					0:11	Construction	0:11	Construction of Fe3O4/SiO2/chitosan-grafted-poly(N-vinylcaprolactam) magnetic nanocomposite and their application in simultaneous extraction of Trans-resveratrol	0:160	Construction of Fe3O4/SiO2/chitosan-grafted-poly(N-vinylcaprolactam) magnetic nanocomposite and their application in simultaneous extraction of Trans-resveratrol and its metabolites from rat plasma.
34225242	8	90	theme	elution	1290:1296	arg1	solvent					1298:1304	elution solvent	1290:1304	elution solvent	1290:1304	Response surface methodology based on central composite design was used to optimize the extraction procedure including pH, amount of adsorbent, extraction time, desorption time, and volume of elution solvent.
34225242	3	91	theme	Fe3O4	473:477	arg1	nanoparticles					488:500	Fe3O4 magnetic nanoparticles	473:500	Fe3O4 magnetic nanoparticles using surface-initiated atom transfer radical polymerization	473:561	N-vinylcaprolactam was first polymerized on the surface of Fe3O4 magnetic nanoparticles using surface-initiated atom transfer radical polymerization.
34225242	6	92	theme	trans-resveratrol	901:917	arg1	extraction					887:896	the simultaneous extraction	870:896	the simultaneous extraction of trans-resveratrol	870:917	The application of Fe3O4/SiO2/CHT-g-PNVCL MNC was utilized as a novel adsorbent for the simultaneous extraction of trans-resveratrol and its major phase II metabolites from rat plasma.
34225242	6	92	theme	trans-resveratrol	901:917	arg1	metabolites					942:952	its major phase II metabolites	923:952	its major phase II metabolites from rat plasma	923:968	The application of Fe3O4/SiO2/CHT-g-PNVCL MNC was utilized as a novel adsorbent for the simultaneous extraction of trans-resveratrol and its major phase II metabolites from rat plasma.
34225242	2	93	theme	deacetylation	390:402	arg1	approach					404:411	a green deacetylation approach	382:411	a green deacetylation approach	382:411	Chitosan was prepared from shrimp shells Penaeus monodon by a green deacetylation approach.
34225242	1	94	theme	Fe3O4/SiO2/CHT-g-PNVCL	276:297	arg1	MNC					299:301	Fe3O4/SiO2/CHT-g-PNVCL MNC	276:301	Fe3O4/SiO2/CHT-g-PNVCL MNC	276:301	A novel magnetic nanocomposite of chitosan-grafted-poly(N-vinylcaprolactam) (Fe3O4/SiO2/CHT-g-PNVCL MNC) were synthesized.
34225242	1	94	theme	Fe3O4/SiO2/CHT-g-PNVCL	276:297	arg1	chitosan-grafted-poly					233:253	chitosan-grafted-poly	233:253	chitosan-grafted-poly(N-vinylcaprolactam) (Fe3O4/SiO2/CHT-g-PNVCL MNC)	233:302	A novel magnetic nanocomposite of chitosan-grafted-poly(N-vinylcaprolactam) (Fe3O4/SiO2/CHT-g-PNVCL MNC) were synthesized.
34225242	8	95	theme	composite	1144:1152	arg1	design					1154:1159	central composite design	1136:1159	central composite design	1136:1159	Response surface methodology based on central composite design was used to optimize the extraction procedure including pH, amount of adsorbent, extraction time, desorption time, and volume of elution solvent.
34225242	7	96	theme	tandem	1072:1077	arg1	spectrometry					1084:1095	ultra-performance liquid chromatography triple-quadrupole tandem mass spectrometry	1014:1095	ultra-performance liquid chromatography triple-quadrupole tandem mass spectrometry	1014:1095	A qualitative analysis was performed using ultra-performance liquid chromatography triple-quadrupole tandem mass spectrometry.
32910163	2	0	from	effects	382:388	arg1	performance					401:411	feedlot performance	393:411	feedlot performance	393:411	Experiments were conducted to evaluate processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence.
32910163	2	0	from	effects	382:388	arg1	characteristics					422:436	carcass characteristics	414:436	carcass characteristics	414:436	Experiments were conducted to evaluate processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence.
32910163	2	0	from	effects	382:388	arg1	incidence					457:465	liver abscess incidence	443:465	liver abscess incidence	443:465	Experiments were conducted to evaluate processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence.
32910163	15	1	theme	greater	2248:2254	arg1	scores					2265:2270	greater marbling scores	2248:2270	greater marbling scores	2248:2270	Cattle fed CON had greater marbling scores than EFC (P = 0.04), but this did not affect the USDA quality grade (P > 0.33).
32910163	12	2	theme	cattle	1920:1925	arg1	EFC					1931:1933	cattle fed EFC	1920:1933	cattle fed EFC	1920:1933	Dry matter intakes were similar between treatments (P = 0.78), but cattle fed EFC had greater average daily gain (P < 0.01), improving feed efficiency by 5% (P < 0.01).
32910163	8	3	theme	total	1250:1254	arg1	VFA					1256:1258	total VFA	1250:1258	total VFA (P < 0.01)	1250:1269	Steam conditioning for 30 min improved starch availability, IVGP, and production of acetate, propionate, butyrate, valerate, and total VFA (P < 0.01) compared with conditioning for 15 or 45 min.
32910163	8	3	theme	total	1250:1254	arg1	<					1263:1263	P < 0.01	1261:1268	P < 0.01	1261:1268	Steam conditioning for 30 min improved starch availability, IVGP, and production of acetate, propionate, butyrate, valerate, and total VFA (P < 0.01) compared with conditioning for 15 or 45 min.
32910163	6	4	theme	or	967:968	arg1	interactions					980:991	No two- or three-way interactions	959:991	No two- or three-way interactions	959:991	No two- or three-way interactions were observed.
32910163	17	5	theme	cattle	2502:2507	arg1	performance					2509:2519	microbial digestion, mill-throughput, and cattle performance	2460:2519	microbial digestion, mill-throughput, and cattle performance	2460:2519	High-amylase corn may be used to improve microbial digestion, mill-throughput, and cattle performance, and it may mitigate liver abscesses.
32910163	2	6	theme	processing	323:332	arg1	characteristics					334:348	processing characteristics	323:348	processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence	323:465	Experiments were conducted to evaluate processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence.
32910163	13	7	theme	EFC	2062:2064	arg1	cattle					2066:2071	EFC cattle	2062:2071	EFC cattle (P <0.01)	2062:2081	Hot carcass weight was 6 kg greater for EFC cattle (P <0.01) than CON.
32910163	13	7	theme	EFC	2062:2064	arg1	<0.01					2076:2080	P <0.01	2074:2080	P <0.01	2074:2080	Hot carcass weight was 6 kg greater for EFC cattle (P <0.01) than CON.
32910163	5	8	theme	steam	917:921	arg1	conditioning					923:934	steam conditioning	917:934	steam conditioning	917:934	Grains were tempered with added moisture (0%, 3%, or 6%) prior to steam conditioning for 15, 30, or 45 min.
32910163	14	9	theme	yield	2205:2209	arg1	grade					2211:2215	USDA yield grade	2200:2215	USDA yield grade (P = 0.13)	2200:2226	No differences were observed for longissimus muscle area (P = 0.89), 12th-rib fat thickness (P = 0.21), or USDA yield grade (P = 0.13).
32910163	14	9	theme	yield	2205:2209	arg1	0.13					2222:2225	P = 0.13	2218:2225	P = 0.13	2218:2225	No differences were observed for longissimus muscle area (P = 0.89), 12th-rib fat thickness (P = 0.21), or USDA yield grade (P = 0.13).
32910163	13	10	theme	P	2074:2074	arg1	cattle					2066:2071	EFC cattle	2062:2071	EFC cattle (P <0.01)	2062:2081	Hot carcass weight was 6 kg greater for EFC cattle (P <0.01) than CON.
32910163	13	10	theme	P	2074:2074	arg1	<0.01					2076:2080	P <0.01	2074:2080	P <0.01	2074:2080	Hot carcass weight was 6 kg greater for EFC cattle (P <0.01) than CON.
32910163	14	11	theme	=	2153:2153	arg1	area					2145:2148	longissimus muscle area	2126:2148	longissimus muscle area (P = 0.89)	2126:2159	No differences were observed for longissimus muscle area (P = 0.89), 12th-rib fat thickness (P = 0.21), or USDA yield grade (P = 0.13).
32910163	14	11	theme	=	2153:2153	arg1	P					2151:2151	P = 0.89	2151:2158	P = 0.89	2151:2158	No differences were observed for longissimus muscle area (P = 0.89), 12th-rib fat thickness (P = 0.21), or USDA yield grade (P = 0.13).
32910163	10	12	theme	d.	1613:1614	arg1	Targeting					1616:1624	136 d. Targeting	1609:1624	136 d. Targeting	1609:1624	Experiment 2 used 700 beef heifers (394 ± 8.5 kg initial body weight [BW]) fed finishing diets with steam-flaked corn as CON or EFC for 136 d. Targeting similar starch availabilities, grains were processed to 360 g/L (CON) and 390 g/L for CON and EFC, respectively.
32910163	3	13	theme	block	508:512	arg1	design					514:519	a randomized complete block design	486:519	a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability	486:573	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	3	13	theme	block	508:512	arg1	factorial					532:540	3 × 3 × 5 factorial	522:540	3 × 3 × 5 factorial	522:540	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	7	14	dep	<	1064:1064	arg1	linear					1054:1059	linear	1054:1059	linear	1054:1059	Adding moisture improved starch availability (linear; P < 0.01), and tended to improve ISDMD (linear, P = 0.06).
32910163	4	15	theme	control	735:741	arg1	Grains					711:716	Grains	711:716	Grains (EFC or mill-run control [CON])	711:748	Grains (EFC or mill-run control [CON]) were flaked to a density of 360 g/L, and mixtures with 0%, 25%, 50%, 75%, or 100% EFC were prepared.
32910163	4	15	theme	control	735:741	arg1	[CON					743:746	mill-run control [CON	726:746	mill-run control [CON	726:746	Grains (EFC or mill-run control [CON]) were flaked to a density of 360 g/L, and mixtures with 0%, 25%, 50%, 75%, or 100% EFC were prepared.
32910163	16	16	contain	had	2367:2369	arg1	EFC					2363:2365	Cattle fed EFC	2352:2365	Cattle fed EFC	2352:2365	Cattle fed EFC had 23% fewer abscessed livers than CON (P = 0.03).
32910163	16	16	contain	had	2367:2369	arg2	livers					2391:2396	23% fewer abscessed livers	2371:2396	23% fewer abscessed livers	2371:2396	Cattle fed EFC had 23% fewer abscessed livers than CON (P = 0.03).
32910163	3	17	theme	volatile	654:661	arg1	profiles					680:687	volatile fatty acid (VFA) profiles	654:687	volatile fatty acid (VFA) profiles of in vitro cultures	654:708	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	14	18	theme	muscle	2138:2143	arg1	area					2145:2148	longissimus muscle area	2126:2148	longissimus muscle area (P = 0.89)	2126:2159	No differences were observed for longissimus muscle area (P = 0.89), 12th-rib fat thickness (P = 0.21), or USDA yield grade (P = 0.13).
32910163	14	18	theme	muscle	2138:2143	arg1	P					2151:2151	P = 0.89	2151:2158	P = 0.89	2151:2158	No differences were observed for longissimus muscle area (P = 0.89), 12th-rib fat thickness (P = 0.21), or USDA yield grade (P = 0.13).
32910163	10	19	theme	beef	1495:1498	arg1	heifers					1500:1506	700 beef heifers	1491:1506	700 beef heifers (394 ± 8.5 kg initial body weight [BW]) fed finishing diets with steam-flaked corn	1491:1589	Experiment 2 used 700 beef heifers (394 ± 8.5 kg initial body weight [BW]) fed finishing diets with steam-flaked corn as CON or EFC for 136 d. Targeting similar starch availabilities, grains were processed to 360 g/L (CON) and 390 g/L for CON and EFC, respectively.
32910163	10	19	theme	beef	1495:1498	arg1	weight					1535:1540	394 ± 8.5 kg initial body weight [BW]	1509:1545	394 ± 8.5 kg initial body weight [BW]	1509:1545	Experiment 2 used 700 beef heifers (394 ± 8.5 kg initial body weight [BW]) fed finishing diets with steam-flaked corn as CON or EFC for 136 d. Targeting similar starch availabilities, grains were processed to 360 g/L (CON) and 390 g/L for CON and EFC, respectively.
32910163	10	20	with	diets	1562:1566	arg1	corn					1586:1589	steam-flaked corn	1573:1589	steam-flaked corn	1573:1589	Experiment 2 used 700 beef heifers (394 ± 8.5 kg initial body weight [BW]) fed finishing diets with steam-flaked corn as CON or EFC for 136 d. Targeting similar starch availabilities, grains were processed to 360 g/L (CON) and 390 g/L for CON and EFC, respectively.
32910163	13	21	theme	Hot	2022:2024	arg1	weight					2034:2039	Hot carcass weight	2022:2039	Hot carcass weight	2022:2039	Hot carcass weight was 6 kg greater for EFC cattle (P <0.01) than CON.
32910163	1	22	theme	Syngenta	170:177	arg1	Protection					184:193	Syngenta Crop Protection	170:193	EFC; Syngenta Crop Protection	165:193	Developed initially for use in fuel ethanol production, Enogen Feed Corn (EFC; Syngenta Crop Protection) is genetically modified to express high concentrations of α-amylase in the corn kernel.
32910163	12	23	contain	had	1935:1937	arg1	EFC					1931:1933	cattle fed EFC	1920:1933	cattle fed EFC	1920:1933	Dry matter intakes were similar between treatments (P = 0.78), but cattle fed EFC had greater average daily gain (P < 0.01), improving feed efficiency by 5% (P < 0.01).
32910163	12	23	contain	had	1935:1937	arg2	gain					1961:1964	greater average daily gain	1939:1964	greater average daily gain (P < 0.01)	1939:1975	Dry matter intakes were similar between treatments (P = 0.78), but cattle fed EFC had greater average daily gain (P < 0.01), improving feed efficiency by 5% (P < 0.01).
32910163	12	23	contain	had	1935:1937	arg2	<					1969:1969	P < 0.01	1967:1974	P < 0.01	1967:1974	Dry matter intakes were similar between treatments (P = 0.78), but cattle fed EFC had greater average daily gain (P < 0.01), improving feed efficiency by 5% (P < 0.01).
32910163	10	24	theme	similar	1626:1632	arg1	availabilities					1641:1654	similar starch availabilities	1626:1654	similar starch availabilities	1626:1654	Experiment 2 used 700 beef heifers (394 ± 8.5 kg initial body weight [BW]) fed finishing diets with steam-flaked corn as CON or EFC for 136 d. Targeting similar starch availabilities, grains were processed to 360 g/L (CON) and 390 g/L for CON and EFC, respectively.
32910163	3	25	theme	VFA	675:677	arg1	profiles					680:687	volatile fatty acid (VFA) profiles	654:687	volatile fatty acid (VFA) profiles of in vitro cultures	654:708	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	3	26	dep	in	692:693	arg1	vitro					695:699	vitro	695:699	vitro	695:699	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	16	27	theme	abscessed	2381:2389	arg1	livers					2391:2396	23% fewer abscessed livers	2371:2396	23% fewer abscessed livers	2371:2396	Cattle fed EFC had 23% fewer abscessed livers than CON (P = 0.03).
32910163	1	28	theme	α-amylase	254:262	arg1	concentrations					236:249	high concentrations	231:249	high concentrations of α-amylase	231:262	Developed initially for use in fuel ethanol production, Enogen Feed Corn (EFC; Syngenta Crop Protection) is genetically modified to express high concentrations of α-amylase in the corn kernel.
32910163	3	29	theme	acid	669:672	arg1	profiles					680:687	volatile fatty acid (VFA) profiles	654:687	volatile fatty acid (VFA) profiles of in vitro cultures	654:708	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	14	30	theme	fat	2171:2173	arg1	thickness					2175:2183	12th-rib fat thickness	2162:2183	12th-rib fat thickness (P = 0.21)	2162:2194	No differences were observed for longissimus muscle area (P = 0.89), 12th-rib fat thickness (P = 0.21), or USDA yield grade (P = 0.13).
32910163	14	30	theme	fat	2171:2173	arg1	0.21					2190:2193	P = 0.21	2186:2193	P = 0.21	2186:2193	No differences were observed for longissimus muscle area (P = 0.89), 12th-rib fat thickness (P = 0.21), or USDA yield grade (P = 0.13).
32910163	12	31	theme	<	2013:2013	arg1	P					2011:2011	P < 0.01	2011:2018	P < 0.01	2011:2018	Dry matter intakes were similar between treatments (P = 0.78), but cattle fed EFC had greater average daily gain (P < 0.01), improving feed efficiency by 5% (P < 0.01).
32910163	12	31	theme	<	2013:2013	arg1	%					2008:2008	5%	2007:2008	5% (P < 0.01)	2007:2019	Dry matter intakes were similar between treatments (P = 0.78), but cattle fed EFC had greater average daily gain (P < 0.01), improving feed efficiency by 5% (P < 0.01).
32910163	3	32	theme	in	692:693	arg1	cultures					701:708	in vitro cultures	692:708	in vitro cultures	692:708	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	15	33	contain	had	2244:2246	arg1	Cattle					2229:2234	Cattle	2229:2234	Cattle fed CON	2229:2242	Cattle fed CON had greater marbling scores than EFC (P = 0.04), but this did not affect the USDA quality grade (P > 0.33).
32910163	15	33	contain	had	2244:2246	arg2	scores					2265:2270	greater marbling scores	2248:2270	greater marbling scores	2248:2270	Cattle fed CON had greater marbling scores than EFC (P = 0.04), but this did not affect the USDA quality grade (P > 0.33).
32910163	1	34	theme	fuel	122:125	arg1	production					135:144	fuel ethanol production	122:144	fuel ethanol production	122:144	Developed initially for use in fuel ethanol production, Enogen Feed Corn (EFC; Syngenta Crop Protection) is genetically modified to express high concentrations of α-amylase in the corn kernel.
32910163	8	35	theme	Steam	1121:1125	arg1	conditioning					1127:1138	Steam conditioning	1121:1138	Steam conditioning for 30 min	1121:1149	Steam conditioning for 30 min improved starch availability, IVGP, and production of acetate, propionate, butyrate, valerate, and total VFA (P < 0.01) compared with conditioning for 15 or 45 min.
32910163	17	36	theme	digestion	2470:2478	arg1	performance					2509:2519	microbial digestion, mill-throughput, and cattle performance	2460:2519	microbial digestion, mill-throughput, and cattle performance	2460:2519	High-amylase corn may be used to improve microbial digestion, mill-throughput, and cattle performance, and it may mitigate liver abscesses.
32910163	10	37	theme	body	1530:1533	arg1	weight					1535:1540	394 ± 8.5 kg initial body weight [BW]	1509:1545	394 ± 8.5 kg initial body weight [BW]	1509:1545	Experiment 2 used 700 beef heifers (394 ± 8.5 kg initial body weight [BW]) fed finishing diets with steam-flaked corn as CON or EFC for 136 d. Targeting similar starch availabilities, grains were processed to 360 g/L (CON) and 390 g/L for CON and EFC, respectively.
32910163	10	37	theme	body	1530:1533	arg1	heifers					1500:1506	700 beef heifers	1491:1506	700 beef heifers (394 ± 8.5 kg initial body weight [BW]) fed finishing diets with steam-flaked corn	1491:1589	Experiment 2 used 700 beef heifers (394 ± 8.5 kg initial body weight [BW]) fed finishing diets with steam-flaked corn as CON or EFC for 136 d. Targeting similar starch availabilities, grains were processed to 360 g/L (CON) and 390 g/L for CON and EFC, respectively.
32910163	3	38	theme	starch	555:560	arg1	availability					562:573	starch availability	555:573	starch availability	555:573	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	16	39	theme	=	2410:2410	arg1	P					2408:2408	P = 0.03	2408:2415	P = 0.03	2408:2415	Cattle fed EFC had 23% fewer abscessed livers than CON (P = 0.03).
32910163	16	39	theme	=	2410:2410	arg1	CON					2403:2405	CON	2403:2405	CON (P = 0.03)	2403:2416	Cattle fed EFC had 23% fewer abscessed livers than CON (P = 0.03).
32910163	15	40	theme	P	2341:2341	arg1	grade					2334:2338	the USDA quality grade	2317:2338	the USDA quality grade (P > 0.33)	2317:2349	Cattle fed CON had greater marbling scores than EFC (P = 0.04), but this did not affect the USDA quality grade (P > 0.33).
32910163	15	40	theme	P	2341:2341	arg1	>					2343:2343	P > 0.33	2341:2348	P > 0.33	2341:2348	Cattle fed CON had greater marbling scores than EFC (P = 0.04), but this did not affect the USDA quality grade (P > 0.33).
32910163	4	41	theme	%	811:811	arg1	EFC					832:834	0%, 25%, 50%, 75%, or 100% EFC	805:834	0%, 25%, 50%, 75%, or 100% EFC	805:834	Grains (EFC or mill-run control [CON]) were flaked to a density of 360 g/L, and mixtures with 0%, 25%, 50%, 75%, or 100% EFC were prepared.
32910163	17	42	theme	mill-throughput	2481:2495	arg1	performance					2509:2519	microbial digestion, mill-throughput, and cattle performance	2460:2519	microbial digestion, mill-throughput, and cattle performance	2460:2519	High-amylase corn may be used to improve microbial digestion, mill-throughput, and cattle performance, and it may mitigate liver abscesses.
32910163	10	43	theme	finishing	1552:1560	arg1	diets					1562:1566	finishing diets	1552:1566	finishing diets with steam-flaked corn	1552:1589	Experiment 2 used 700 beef heifers (394 ± 8.5 kg initial body weight [BW]) fed finishing diets with steam-flaked corn as CON or EFC for 136 d. Targeting similar starch availabilities, grains were processed to 360 g/L (CON) and 390 g/L for CON and EFC, respectively.
32910163	0	44	theme	finishing	67:75	arg1	heifers					82:88	finishing beef heifers	67:88	finishing beef heifers	67:88	Effects of high-amylase corn on performance and carcass quality of finishing beef heifers.
32910163	1	45	theme	Enogen	147:152	arg1	EFC					165:167	EFC	165:167	EFC; Syngenta Crop Protection	165:193	Developed initially for use in fuel ethanol production, Enogen Feed Corn (EFC; Syngenta Crop Protection) is genetically modified to express high concentrations of α-amylase in the corn kernel.
32910163	1	45	theme	Enogen	147:152	arg1	Corn					159:162	Enogen Feed Corn	147:162	Enogen Feed Corn (EFC; Syngenta Crop Protection)	147:194	Developed initially for use in fuel ethanol production, Enogen Feed Corn (EFC; Syngenta Crop Protection) is genetically modified to express high concentrations of α-amylase in the corn kernel.
32910163	0	46	from	Effects	0:6	arg1	performance					32:42	performance	32:42	performance	32:42	Effects of high-amylase corn on performance and carcass quality of finishing beef heifers.
32910163	0	46	from	Effects	0:6	arg1	quality					56:62	carcass quality	48:62	carcass quality	48:62	Effects of high-amylase corn on performance and carcass quality of finishing beef heifers.
32910163	10	47	theme	390	1700:1702	arg1	g/L					1704:1706	390 g/L	1700:1706	390 g/L	1700:1706	Experiment 2 used 700 beef heifers (394 ± 8.5 kg initial body weight [BW]) fed finishing diets with steam-flaked corn as CON or EFC for 136 d. Targeting similar starch availabilities, grains were processed to 360 g/L (CON) and 390 g/L for CON and EFC, respectively.
32910163	3	48	theme	cultures	701:708	arg1	IVGP					643:646	IVGP	643:646	IVGP	643:646	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	3	48	theme	cultures	701:708	arg1	disappearance					595:607	in situ dry matter disappearance	576:607	in situ dry matter disappearance (ISDMD)	576:615	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	3	48	theme	cultures	701:708	arg1	design					514:519	a randomized complete block design	486:519	a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability	486:573	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	3	48	theme	cultures	701:708	arg1	profiles					680:687	volatile fatty acid (VFA) profiles	654:687	volatile fatty acid (VFA) profiles of in vitro cultures	654:708	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	3	48	theme	cultures	701:708	arg1	ISDMD					610:614	ISDMD	610:614	ISDMD	610:614	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	3	48	theme	cultures	701:708	arg1	factorial					532:540	3 × 3 × 5 factorial	522:540	3 × 3 × 5 factorial	522:540	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	3	48	theme	cultures	701:708	arg1	production					631:640	in vitro gas production	618:640	in vitro gas production (IVGP)	618:647	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	0	49	theme	heifers	82:88	arg1	performance					32:42	performance	32:42	performance	32:42	Effects of high-amylase corn on performance and carcass quality of finishing beef heifers.
32910163	0	49	theme	heifers	82:88	arg1	quality					56:62	carcass quality	48:62	carcass quality	48:62	Effects of high-amylase corn on performance and carcass quality of finishing beef heifers.
32910163	16	50	theme	fed	2359:2361	arg1	EFC					2363:2365	Cattle fed EFC	2352:2365	Cattle fed EFC	2352:2365	Cattle fed EFC had 23% fewer abscessed livers than CON (P = 0.03).
32910163	15	51	theme	USDA	2321:2324	arg1	grade					2334:2338	the USDA quality grade	2317:2338	the USDA quality grade (P > 0.33)	2317:2349	Cattle fed CON had greater marbling scores than EFC (P = 0.04), but this did not affect the USDA quality grade (P > 0.33).
32910163	15	51	theme	USDA	2321:2324	arg1	>					2343:2343	P > 0.33	2341:2348	P > 0.33	2341:2348	Cattle fed CON had greater marbling scores than EFC (P = 0.04), but this did not affect the USDA quality grade (P > 0.33).
32910163	2	52	from	characteristics	422:436	arg1	characteristics					334:348	processing characteristics	323:348	processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence	323:465	Experiments were conducted to evaluate processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence.
32910163	11	53	theme	dirt-surfaced	1812:1824	arg1	pens					1826:1829	28 dirt-surfaced pens	1809:1829	28 dirt-surfaced pens (25 animals per pen)	1809:1850	Heifers were blocked by BW, stratified, and then randomly assigned to 28 dirt-surfaced pens (25 animals per pen).
32910163	11	53	theme	dirt-surfaced	1812:1824	arg1	animals					1835:1841	25 animals per pen	1832:1849	25 animals per pen	1832:1849	Heifers were blocked by BW, stratified, and then randomly assigned to 28 dirt-surfaced pens (25 animals per pen).
32910163	2	54	theme	digestion	367:375	arg1	characteristics					334:348	processing characteristics	323:348	processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence	323:465	Experiments were conducted to evaluate processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence.
32910163	5	55	theme	added	877:881	arg1	moisture					883:890	added moisture	877:890	added moisture (0%, 3%, or 6%)	877:906	Grains were tempered with added moisture (0%, 3%, or 6%) prior to steam conditioning for 15, 30, or 45 min.
32910163	2	56	theme	liver	443:447	arg1	incidence					457:465	liver abscess incidence	443:465	liver abscess incidence	443:465	Experiments were conducted to evaluate processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence.
32910163	9	57	dep	linear	1454:1459	arg1	<					1464:1464	P < 0.01	1462:1469	P < 0.01	1462:1469	Starch availability, ISDMD, IVGP, acetate, propionate, valerate, and total VFA production increased with an increasing proportion of EFC (linear, P < 0.01).
32910163	7	58	theme	starch	1033:1038	arg1	availability					1040:1051	starch availability	1033:1051	starch availability	1033:1051	Adding moisture improved starch availability (linear; P < 0.01), and tended to improve ISDMD (linear, P = 0.06).
32910163	4	59	theme	%	821:821	arg1	EFC					832:834	0%, 25%, 50%, 75%, or 100% EFC	805:834	0%, 25%, 50%, 75%, or 100% EFC	805:834	Grains (EFC or mill-run control [CON]) were flaked to a density of 360 g/L, and mixtures with 0%, 25%, 50%, 75%, or 100% EFC were prepared.
32910163	11	60	theme	pen	1847:1849	arg1	pens					1826:1829	28 dirt-surfaced pens	1809:1829	28 dirt-surfaced pens (25 animals per pen)	1809:1850	Heifers were blocked by BW, stratified, and then randomly assigned to 28 dirt-surfaced pens (25 animals per pen).
32910163	11	60	theme	pen	1847:1849	arg1	animals					1835:1841	25 animals per pen	1832:1849	25 animals per pen	1832:1849	Heifers were blocked by BW, stratified, and then randomly assigned to 28 dirt-surfaced pens (25 animals per pen).
32910163	17	61	theme	High-amylase	2419:2430	arg1	corn					2432:2435	High-amylase corn	2419:2435	High-amylase corn	2419:2435	High-amylase corn may be used to improve microbial digestion, mill-throughput, and cattle performance, and it may mitigate liver abscesses.
32910163	2	62	theme	in	358:359	arg1	digestion					367:375	in vitro digestion	358:375	in vitro digestion	358:375	Experiments were conducted to evaluate processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence.
32910163	12	63	theme	fed	1927:1929	arg1	EFC					1931:1933	cattle fed EFC	1920:1933	cattle fed EFC	1920:1933	Dry matter intakes were similar between treatments (P = 0.78), but cattle fed EFC had greater average daily gain (P < 0.01), improving feed efficiency by 5% (P < 0.01).
32910163	14	64	theme	USDA	2200:2203	arg1	grade					2211:2215	USDA yield grade	2200:2215	USDA yield grade (P = 0.13)	2200:2226	No differences were observed for longissimus muscle area (P = 0.89), 12th-rib fat thickness (P = 0.21), or USDA yield grade (P = 0.13).
32910163	14	64	theme	USDA	2200:2203	arg1	0.13					2222:2225	P = 0.13	2218:2225	P = 0.13	2218:2225	No differences were observed for longissimus muscle area (P = 0.89), 12th-rib fat thickness (P = 0.21), or USDA yield grade (P = 0.13).
32910163	0	65	theme	high-amylase	11:22	arg1	corn					24:27	high-amylase corn	11:27	high-amylase corn	11:27	Effects of high-amylase corn on performance and carcass quality of finishing beef heifers.
32910163	9	66	theme	VFA	1391:1393	arg1	production					1395:1404	total VFA production	1385:1404	total VFA production	1385:1404	Starch availability, ISDMD, IVGP, acetate, propionate, valerate, and total VFA production increased with an increasing proportion of EFC (linear, P < 0.01).
32910163	3	67	theme	matter	588:593	arg1	ISDMD					610:614	ISDMD	610:614	ISDMD	610:614	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	3	67	theme	matter	588:593	arg1	disappearance					595:607	in situ dry matter disappearance	576:607	in situ dry matter disappearance (ISDMD)	576:615	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	1	68	theme	corn	271:274	arg1	kernel					276:281	the corn kernel	267:281	the corn kernel	267:281	Developed initially for use in fuel ethanol production, Enogen Feed Corn (EFC; Syngenta Crop Protection) is genetically modified to express high concentrations of α-amylase in the corn kernel.
32910163	8	69	theme	acetate	1205:1211	arg1	production					1191:1200	production	1191:1200	production of acetate, propionate, butyrate, valerate, and total VFA (P < 0.01)	1191:1269	Steam conditioning for 30 min improved starch availability, IVGP, and production of acetate, propionate, butyrate, valerate, and total VFA (P < 0.01) compared with conditioning for 15 or 45 min.
32910163	8	69	theme	acetate	1205:1211	arg1	availability					1167:1178	starch availability	1160:1178	starch availability	1160:1178	Steam conditioning for 30 min improved starch availability, IVGP, and production of acetate, propionate, butyrate, valerate, and total VFA (P < 0.01) compared with conditioning for 15 or 45 min.
32910163	8	69	theme	acetate	1205:1211	arg1	IVGP					1181:1184	IVGP	1181:1184	IVGP	1181:1184	Steam conditioning for 30 min improved starch availability, IVGP, and production of acetate, propionate, butyrate, valerate, and total VFA (P < 0.01) compared with conditioning for 15 or 45 min.
32910163	16	70	theme	Cattle	2352:2357	arg1	EFC					2363:2365	Cattle fed EFC	2352:2365	Cattle fed EFC	2352:2365	Cattle fed EFC had 23% fewer abscessed livers than CON (P = 0.03).
32910163	14	71	theme	=	2220:2220	arg1	grade					2211:2215	USDA yield grade	2200:2215	USDA yield grade (P = 0.13)	2200:2226	No differences were observed for longissimus muscle area (P = 0.89), 12th-rib fat thickness (P = 0.21), or USDA yield grade (P = 0.13).
32910163	14	71	theme	=	2220:2220	arg1	0.13					2222:2225	P = 0.13	2218:2225	P = 0.13	2218:2225	No differences were observed for longissimus muscle area (P = 0.89), 12th-rib fat thickness (P = 0.21), or USDA yield grade (P = 0.13).
32910163	7	72	dep	=	1112:1112	arg1	linear					1102:1107	linear	1102:1107	linear	1102:1107	Adding moisture improved starch availability (linear; P < 0.01), and tended to improve ISDMD (linear, P = 0.06).
32910163	2	73	from	digestion	367:375	arg1	performance					401:411	feedlot performance	393:411	feedlot performance	393:411	Experiments were conducted to evaluate processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence.
32910163	2	73	from	digestion	367:375	arg1	characteristics					422:436	carcass characteristics	414:436	carcass characteristics	414:436	Experiments were conducted to evaluate processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence.
32910163	2	73	from	digestion	367:375	arg1	incidence					457:465	liver abscess incidence	443:465	liver abscess incidence	443:465	Experiments were conducted to evaluate processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence.
32910163	15	74	theme	=	2284:2284	arg1	P					2282:2282	P = 0.04	2282:2289	P = 0.04	2282:2289	Cattle fed CON had greater marbling scores than EFC (P = 0.04), but this did not affect the USDA quality grade (P > 0.33).
32910163	15	74	theme	=	2284:2284	arg1	EFC					2277:2279	EFC	2277:2279	EFC (P = 0.04)	2277:2290	Cattle fed CON had greater marbling scores than EFC (P = 0.04), but this did not affect the USDA quality grade (P > 0.33).
32910163	12	75	theme	average	1947:1953	arg1	<					1969:1969	P < 0.01	1967:1974	P < 0.01	1967:1974	Dry matter intakes were similar between treatments (P = 0.78), but cattle fed EFC had greater average daily gain (P < 0.01), improving feed efficiency by 5% (P < 0.01).
32910163	12	75	theme	average	1947:1953	arg1	gain					1961:1964	greater average daily gain	1939:1964	greater average daily gain (P < 0.01)	1939:1975	Dry matter intakes were similar between treatments (P = 0.78), but cattle fed EFC had greater average daily gain (P < 0.01), improving feed efficiency by 5% (P < 0.01).
32910163	8	76	theme	propionate	1214:1223	arg1	production					1191:1200	production	1191:1200	production of acetate, propionate, butyrate, valerate, and total VFA (P < 0.01)	1191:1269	Steam conditioning for 30 min improved starch availability, IVGP, and production of acetate, propionate, butyrate, valerate, and total VFA (P < 0.01) compared with conditioning for 15 or 45 min.
32910163	8	76	theme	propionate	1214:1223	arg1	availability					1167:1178	starch availability	1160:1178	starch availability	1160:1178	Steam conditioning for 30 min improved starch availability, IVGP, and production of acetate, propionate, butyrate, valerate, and total VFA (P < 0.01) compared with conditioning for 15 or 45 min.
32910163	8	76	theme	propionate	1214:1223	arg1	IVGP					1181:1184	IVGP	1181:1184	IVGP	1181:1184	Steam conditioning for 30 min improved starch availability, IVGP, and production of acetate, propionate, butyrate, valerate, and total VFA (P < 0.01) compared with conditioning for 15 or 45 min.
32910163	3	77	used	used	481:484	arg2	Experiment					468:477	Experiment 1	468:479	Experiment 1	468:479	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	8	78	theme	P	1261:1261	arg1	VFA					1256:1258	total VFA	1250:1258	total VFA (P < 0.01)	1250:1269	Steam conditioning for 30 min improved starch availability, IVGP, and production of acetate, propionate, butyrate, valerate, and total VFA (P < 0.01) compared with conditioning for 15 or 45 min.
32910163	8	78	theme	P	1261:1261	arg1	<					1263:1263	P < 0.01	1261:1268	P < 0.01	1261:1268	Steam conditioning for 30 min improved starch availability, IVGP, and production of acetate, propionate, butyrate, valerate, and total VFA (P < 0.01) compared with conditioning for 15 or 45 min.
32910163	3	79	dep	in	576:577	arg1	situ					579:582	situ	579:582	situ	579:582	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	8	80	theme	butyrate	1226:1233	arg1	production					1191:1200	production	1191:1200	production of acetate, propionate, butyrate, valerate, and total VFA (P < 0.01)	1191:1269	Steam conditioning for 30 min improved starch availability, IVGP, and production of acetate, propionate, butyrate, valerate, and total VFA (P < 0.01) compared with conditioning for 15 or 45 min.
32910163	8	80	theme	butyrate	1226:1233	arg1	availability					1167:1178	starch availability	1160:1178	starch availability	1160:1178	Steam conditioning for 30 min improved starch availability, IVGP, and production of acetate, propionate, butyrate, valerate, and total VFA (P < 0.01) compared with conditioning for 15 or 45 min.
32910163	8	80	theme	butyrate	1226:1233	arg1	IVGP					1181:1184	IVGP	1181:1184	IVGP	1181:1184	Steam conditioning for 30 min improved starch availability, IVGP, and production of acetate, propionate, butyrate, valerate, and total VFA (P < 0.01) compared with conditioning for 15 or 45 min.
32910163	2	81	theme	EFC	353:355	arg1	characteristics					334:348	processing characteristics	323:348	processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence	323:465	Experiments were conducted to evaluate processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence.
32910163	4	82	with	mixtures	791:798	arg1	EFC					832:834	0%, 25%, 50%, 75%, or 100% EFC	805:834	0%, 25%, 50%, 75%, or 100% EFC	805:834	Grains (EFC or mill-run control [CON]) were flaked to a density of 360 g/L, and mixtures with 0%, 25%, 50%, 75%, or 100% EFC were prepared.
32910163	9	83	theme	EFC	1449:1451	arg1	proportion					1435:1444	an increasing proportion	1421:1444	an increasing proportion of EFC	1421:1451	Starch availability, ISDMD, IVGP, acetate, propionate, valerate, and total VFA production increased with an increasing proportion of EFC (linear, P < 0.01).
32910163	5	84	dep	moisture	883:890	arg1	%					905:905	6%	904:905	6%	904:905	Grains were tempered with added moisture (0%, 3%, or 6%) prior to steam conditioning for 15, 30, or 45 min.
32910163	5	84	dep	moisture	883:890	arg1	%					894:894	0%	893:894	0%	893:894	Grains were tempered with added moisture (0%, 3%, or 6%) prior to steam conditioning for 15, 30, or 45 min.
32910163	5	84	dep	moisture	883:890	arg1	%					898:898	3%	897:898	3%	897:898	Grains were tempered with added moisture (0%, 3%, or 6%) prior to steam conditioning for 15, 30, or 45 min.
32910163	3	85	theme	gas	627:629	arg1	IVGP					643:646	IVGP	643:646	IVGP	643:646	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	3	85	theme	gas	627:629	arg1	production					631:640	in vitro gas production	618:640	in vitro gas production (IVGP)	618:647	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	6	86	theme	three-way	970:978	arg1	interactions					980:991	No two- or three-way interactions	959:991	No two- or three-way interactions	959:991	No two- or three-way interactions were observed.
32910163	14	87	theme	=	2188:2188	arg1	thickness					2175:2183	12th-rib fat thickness	2162:2183	12th-rib fat thickness (P = 0.21)	2162:2194	No differences were observed for longissimus muscle area (P = 0.89), 12th-rib fat thickness (P = 0.21), or USDA yield grade (P = 0.13).
32910163	14	87	theme	=	2188:2188	arg1	0.21					2190:2193	P = 0.21	2186:2193	P = 0.21	2186:2193	No differences were observed for longissimus muscle area (P = 0.89), 12th-rib fat thickness (P = 0.21), or USDA yield grade (P = 0.13).
32910163	10	88	dep	CON	1594:1596	arg1	availabilities					1641:1654	similar starch availabilities	1626:1654	similar starch availabilities	1626:1654	Experiment 2 used 700 beef heifers (394 ± 8.5 kg initial body weight [BW]) fed finishing diets with steam-flaked corn as CON or EFC for 136 d. Targeting similar starch availabilities, grains were processed to 360 g/L (CON) and 390 g/L for CON and EFC, respectively.
32910163	15	89	theme	marbling	2256:2263	arg1	scores					2265:2270	greater marbling scores	2248:2270	greater marbling scores	2248:2270	Cattle fed CON had greater marbling scores than EFC (P = 0.04), but this did not affect the USDA quality grade (P > 0.33).
32910163	3	90	theme	in	618:619	arg1	IVGP					643:646	IVGP	643:646	IVGP	643:646	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	3	90	theme	in	618:619	arg1	production					631:640	in vitro gas production	618:640	in vitro gas production (IVGP)	618:647	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	1	91	theme	high	231:234	arg1	concentrations					236:249	high concentrations	231:249	high concentrations of α-amylase	231:262	Developed initially for use in fuel ethanol production, Enogen Feed Corn (EFC; Syngenta Crop Protection) is genetically modified to express high concentrations of α-amylase in the corn kernel.
32910163	9	92	theme	P	1462:1462	arg1	<					1464:1464	P < 0.01	1462:1469	P < 0.01	1462:1469	Starch availability, ISDMD, IVGP, acetate, propionate, valerate, and total VFA production increased with an increasing proportion of EFC (linear, P < 0.01).
32910163	8	93	theme	VFA	1256:1258	arg1	production					1191:1200	production	1191:1200	production of acetate, propionate, butyrate, valerate, and total VFA (P < 0.01)	1191:1269	Steam conditioning for 30 min improved starch availability, IVGP, and production of acetate, propionate, butyrate, valerate, and total VFA (P < 0.01) compared with conditioning for 15 or 45 min.
32910163	8	93	theme	VFA	1256:1258	arg1	availability					1167:1178	starch availability	1160:1178	starch availability	1160:1178	Steam conditioning for 30 min improved starch availability, IVGP, and production of acetate, propionate, butyrate, valerate, and total VFA (P < 0.01) compared with conditioning for 15 or 45 min.
32910163	8	93	theme	VFA	1256:1258	arg1	IVGP					1181:1184	IVGP	1181:1184	IVGP	1181:1184	Steam conditioning for 30 min improved starch availability, IVGP, and production of acetate, propionate, butyrate, valerate, and total VFA (P < 0.01) compared with conditioning for 15 or 45 min.
32910163	9	94	dep	increased	1406:1414	arg1	linear					1454:1459	linear	1454:1459	linear	1454:1459	Starch availability, ISDMD, IVGP, acetate, propionate, valerate, and total VFA production increased with an increasing proportion of EFC (linear, P < 0.01).
32910163	3	95	theme	complete	499:506	arg1	design					514:519	a randomized complete block design	486:519	a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability	486:573	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	3	95	theme	complete	499:506	arg1	factorial					532:540	3 × 3 × 5 factorial	522:540	3 × 3 × 5 factorial	522:540	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	1	96	theme	Feed	154:157	arg1	EFC					165:167	EFC	165:167	EFC; Syngenta Crop Protection	165:193	Developed initially for use in fuel ethanol production, Enogen Feed Corn (EFC; Syngenta Crop Protection) is genetically modified to express high concentrations of α-amylase in the corn kernel.
32910163	1	96	theme	Feed	154:157	arg1	Corn					159:162	Enogen Feed Corn	147:162	Enogen Feed Corn (EFC; Syngenta Crop Protection)	147:194	Developed initially for use in fuel ethanol production, Enogen Feed Corn (EFC; Syngenta Crop Protection) is genetically modified to express high concentrations of α-amylase in the corn kernel.
32910163	2	97	from	characteristics	334:348	arg1	performance					401:411	feedlot performance	393:411	feedlot performance	393:411	Experiments were conducted to evaluate processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence.
32910163	2	97	from	characteristics	334:348	arg1	characteristics					422:436	carcass characteristics	414:436	carcass characteristics	414:436	Experiments were conducted to evaluate processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence.
32910163	2	97	from	characteristics	334:348	arg1	incidence					457:465	liver abscess incidence	443:465	liver abscess incidence	443:465	Experiments were conducted to evaluate processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence.
32910163	4	98	theme	mill-run	726:733	arg1	Grains					711:716	Grains	711:716	Grains (EFC or mill-run control [CON])	711:748	Grains (EFC or mill-run control [CON]) were flaked to a density of 360 g/L, and mixtures with 0%, 25%, 50%, 75%, or 100% EFC were prepared.
32910163	4	98	theme	mill-run	726:733	arg1	[CON					743:746	mill-run control [CON	726:746	mill-run control [CON	726:746	Grains (EFC or mill-run control [CON]) were flaked to a density of 360 g/L, and mixtures with 0%, 25%, 50%, 75%, or 100% EFC were prepared.
32910163	2	99	from	EFC	353:355	arg1	performance					401:411	feedlot performance	393:411	feedlot performance	393:411	Experiments were conducted to evaluate processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence.
32910163	2	99	from	EFC	353:355	arg1	characteristics					422:436	carcass characteristics	414:436	carcass characteristics	414:436	Experiments were conducted to evaluate processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence.
32910163	2	99	from	EFC	353:355	arg1	incidence					457:465	liver abscess incidence	443:465	liver abscess incidence	443:465	Experiments were conducted to evaluate processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence.
32910163	3	100	theme	fatty	663:667	arg1	profiles					680:687	volatile fatty acid (VFA) profiles	654:687	volatile fatty acid (VFA) profiles of in vitro cultures	654:708	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	17	101	theme	liver	2542:2546	arg1	abscesses					2548:2556	liver abscesses	2542:2556	liver abscesses	2542:2556	High-amylase corn may be used to improve microbial digestion, mill-throughput, and cattle performance, and it may mitigate liver abscesses.
32910163	2	102	from	incidence	457:465	arg1	characteristics					334:348	processing characteristics	323:348	processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence	323:465	Experiments were conducted to evaluate processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence.
32910163	14	103	theme	longissimus	2126:2136	arg1	area					2145:2148	longissimus muscle area	2126:2148	longissimus muscle area (P = 0.89)	2126:2159	No differences were observed for longissimus muscle area (P = 0.89), 12th-rib fat thickness (P = 0.21), or USDA yield grade (P = 0.13).
32910163	14	103	theme	longissimus	2126:2136	arg1	P					2151:2151	P = 0.89	2151:2158	P = 0.89	2151:2158	No differences were observed for longissimus muscle area (P = 0.89), 12th-rib fat thickness (P = 0.21), or USDA yield grade (P = 0.13).
32910163	10	104	theme	starch	1634:1639	arg1	availabilities					1641:1654	similar starch availabilities	1626:1654	similar starch availabilities	1626:1654	Experiment 2 used 700 beef heifers (394 ± 8.5 kg initial body weight [BW]) fed finishing diets with steam-flaked corn as CON or EFC for 136 d. Targeting similar starch availabilities, grains were processed to 360 g/L (CON) and 390 g/L for CON and EFC, respectively.
32910163	14	105	theme	P	2186:2186	arg1	thickness					2175:2183	12th-rib fat thickness	2162:2183	12th-rib fat thickness (P = 0.21)	2162:2194	No differences were observed for longissimus muscle area (P = 0.89), 12th-rib fat thickness (P = 0.21), or USDA yield grade (P = 0.13).
32910163	14	105	theme	P	2186:2186	arg1	0.21					2190:2193	P = 0.21	2186:2193	P = 0.21	2186:2193	No differences were observed for longissimus muscle area (P = 0.89), 12th-rib fat thickness (P = 0.21), or USDA yield grade (P = 0.13).
32910163	13	106	theme	carcass	2026:2032	arg1	weight					2034:2039	Hot carcass weight	2022:2039	Hot carcass weight	2022:2039	Hot carcass weight was 6 kg greater for EFC cattle (P <0.01) than CON.
32910163	1	107	theme	Crop	179:182	arg1	Protection					184:193	Syngenta Crop Protection	170:193	EFC; Syngenta Crop Protection	165:193	Developed initially for use in fuel ethanol production, Enogen Feed Corn (EFC; Syngenta Crop Protection) is genetically modified to express high concentrations of α-amylase in the corn kernel.
32910163	4	108	theme	g/L	782:784	arg1	density					767:773	a density	765:773	a density of 360 g/L	765:784	Grains (EFC or mill-run control [CON]) were flaked to a density of 360 g/L, and mixtures with 0%, 25%, 50%, 75%, or 100% EFC were prepared.
32910163	12	109	theme	Dry	1853:1855	arg1	intakes					1864:1870	Dry matter intakes	1853:1870	Dry matter intakes	1853:1870	Dry matter intakes were similar between treatments (P = 0.78), but cattle fed EFC had greater average daily gain (P < 0.01), improving feed efficiency by 5% (P < 0.01).
32910163	9	110	theme	total	1385:1389	arg1	production					1395:1404	total VFA production	1385:1404	total VFA production	1385:1404	Starch availability, ISDMD, IVGP, acetate, propionate, valerate, and total VFA production increased with an increasing proportion of EFC (linear, P < 0.01).
32910163	16	111	theme	fewer	2375:2379	arg1	livers					2391:2396	23% fewer abscessed livers	2371:2396	23% fewer abscessed livers	2371:2396	Cattle fed EFC had 23% fewer abscessed livers than CON (P = 0.03).
32910163	9	112	theme	Starch	1316:1321	arg1	availability					1323:1334	Starch availability	1316:1334	Starch availability	1316:1334	Starch availability, ISDMD, IVGP, acetate, propionate, valerate, and total VFA production increased with an increasing proportion of EFC (linear, P < 0.01).
32910163	14	113	theme	12th-rib	2162:2169	arg1	thickness					2175:2183	12th-rib fat thickness	2162:2183	12th-rib fat thickness (P = 0.21)	2162:2194	No differences were observed for longissimus muscle area (P = 0.89), 12th-rib fat thickness (P = 0.21), or USDA yield grade (P = 0.13).
32910163	14	113	theme	12th-rib	2162:2169	arg1	0.21					2190:2193	P = 0.21	2186:2193	P = 0.21	2186:2193	No differences were observed for longissimus muscle area (P = 0.89), 12th-rib fat thickness (P = 0.21), or USDA yield grade (P = 0.13).
32910163	2	114	dep	in	358:359	arg1	vitro					361:365	vitro	361:365	vitro	361:365	Experiments were conducted to evaluate processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence.
32910163	12	115	theme	greater	1939:1945	arg1	<					1969:1969	P < 0.01	1967:1974	P < 0.01	1967:1974	Dry matter intakes were similar between treatments (P = 0.78), but cattle fed EFC had greater average daily gain (P < 0.01), improving feed efficiency by 5% (P < 0.01).
32910163	12	115	theme	greater	1939:1945	arg1	gain					1961:1964	greater average daily gain	1939:1964	greater average daily gain (P < 0.01)	1939:1975	Dry matter intakes were similar between treatments (P = 0.78), but cattle fed EFC had greater average daily gain (P < 0.01), improving feed efficiency by 5% (P < 0.01).
32910163	17	116	theme	microbial	2460:2468	arg1	performance					2509:2519	microbial digestion, mill-throughput, and cattle performance	2460:2519	microbial digestion, mill-throughput, and cattle performance	2460:2519	High-amylase corn may be used to improve microbial digestion, mill-throughput, and cattle performance, and it may mitigate liver abscesses.
32910163	1	117	theme	ethanol	127:133	arg1	production					135:144	fuel ethanol production	122:144	fuel ethanol production	122:144	Developed initially for use in fuel ethanol production, Enogen Feed Corn (EFC; Syngenta Crop Protection) is genetically modified to express high concentrations of α-amylase in the corn kernel.
32910163	10	118	theme	steam-flaked	1573:1584	arg1	corn					1586:1589	steam-flaked corn	1573:1589	steam-flaked corn	1573:1589	Experiment 2 used 700 beef heifers (394 ± 8.5 kg initial body weight [BW]) fed finishing diets with steam-flaked corn as CON or EFC for 136 d. Targeting similar starch availabilities, grains were processed to 360 g/L (CON) and 390 g/L for CON and EFC, respectively.
32910163	4	119	theme	%	806:806	arg1	EFC					832:834	0%, 25%, 50%, 75%, or 100% EFC	805:834	0%, 25%, 50%, 75%, or 100% EFC	805:834	Grains (EFC or mill-run control [CON]) were flaked to a density of 360 g/L, and mixtures with 0%, 25%, 50%, 75%, or 100% EFC were prepared.
32910163	2	120	from	performance	401:411	arg1	characteristics					334:348	processing characteristics	323:348	processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence	323:465	Experiments were conducted to evaluate processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence.
32910163	10	121	theme	initial	1522:1528	arg1	weight					1535:1540	394 ± 8.5 kg initial body weight [BW]	1509:1545	394 ± 8.5 kg initial body weight [BW]	1509:1545	Experiment 2 used 700 beef heifers (394 ± 8.5 kg initial body weight [BW]) fed finishing diets with steam-flaked corn as CON or EFC for 136 d. Targeting similar starch availabilities, grains were processed to 360 g/L (CON) and 390 g/L for CON and EFC, respectively.
32910163	10	121	theme	initial	1522:1528	arg1	heifers					1500:1506	700 beef heifers	1491:1506	700 beef heifers (394 ± 8.5 kg initial body weight [BW]) fed finishing diets with steam-flaked corn	1491:1589	Experiment 2 used 700 beef heifers (394 ± 8.5 kg initial body weight [BW]) fed finishing diets with steam-flaked corn as CON or EFC for 136 d. Targeting similar starch availabilities, grains were processed to 360 g/L (CON) and 390 g/L for CON and EFC, respectively.
32910163	0	122	theme	beef	77:80	arg1	heifers					82:88	finishing beef heifers	67:88	finishing beef heifers	67:88	Effects of high-amylase corn on performance and carcass quality of finishing beef heifers.
32910163	15	123	theme	quality	2326:2332	arg1	grade					2334:2338	the USDA quality grade	2317:2338	the USDA quality grade (P > 0.33)	2317:2349	Cattle fed CON had greater marbling scores than EFC (P = 0.04), but this did not affect the USDA quality grade (P > 0.33).
32910163	15	123	theme	quality	2326:2332	arg1	>					2343:2343	P > 0.33	2341:2348	P > 0.33	2341:2348	Cattle fed CON had greater marbling scores than EFC (P = 0.04), but this did not affect the USDA quality grade (P > 0.33).
32910163	2	124	theme	abscess	449:455	arg1	incidence					457:465	liver abscess incidence	443:465	liver abscess incidence	443:465	Experiments were conducted to evaluate processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence.
32910163	12	125	theme	feed	1988:1991	arg1	efficiency					1993:2002	feed efficiency	1988:2002	feed efficiency	1988:2002	Dry matter intakes were similar between treatments (P = 0.78), but cattle fed EFC had greater average daily gain (P < 0.01), improving feed efficiency by 5% (P < 0.01).
32910163	12	126	theme	=	1907:1907	arg1	treatments					1893:1902	treatments	1893:1902	treatments (P = 0.78)	1893:1913	Dry matter intakes were similar between treatments (P = 0.78), but cattle fed EFC had greater average daily gain (P < 0.01), improving feed efficiency by 5% (P < 0.01).
32910163	12	126	theme	=	1907:1907	arg1	0.78					1909:1912	P = 0.78	1905:1912	P = 0.78	1905:1912	Dry matter intakes were similar between treatments (P = 0.78), but cattle fed EFC had greater average daily gain (P < 0.01), improving feed efficiency by 5% (P < 0.01).
32910163	4	127	dep	Grains	711:716	arg1	EFC					719:721	EFC	719:721	EFC	719:721	Grains (EFC or mill-run control [CON]) were flaked to a density of 360 g/L, and mixtures with 0%, 25%, 50%, 75%, or 100% EFC were prepared.
32910163	4	127	dep	Grains	711:716	arg1	Grains					711:716	Grains	711:716	Grains (EFC or mill-run control [CON])	711:748	Grains (EFC or mill-run control [CON]) were flaked to a density of 360 g/L, and mixtures with 0%, 25%, 50%, 75%, or 100% EFC were prepared.
32910163	4	127	dep	Grains	711:716	arg1	[CON					743:746	mill-run control [CON	726:746	mill-run control [CON	726:746	Grains (EFC or mill-run control [CON]) were flaked to a density of 360 g/L, and mixtures with 0%, 25%, 50%, 75%, or 100% EFC were prepared.
32910163	11	128	theme	per	1843:1845	arg1	pens					1826:1829	28 dirt-surfaced pens	1809:1829	28 dirt-surfaced pens (25 animals per pen)	1809:1850	Heifers were blocked by BW, stratified, and then randomly assigned to 28 dirt-surfaced pens (25 animals per pen).
32910163	11	128	theme	per	1843:1845	arg1	animals					1835:1841	25 animals per pen	1832:1849	25 animals per pen	1832:1849	Heifers were blocked by BW, stratified, and then randomly assigned to 28 dirt-surfaced pens (25 animals per pen).
32910163	8	129	theme	starch	1160:1165	arg1	availability					1167:1178	starch availability	1160:1178	starch availability	1160:1178	Steam conditioning for 30 min improved starch availability, IVGP, and production of acetate, propionate, butyrate, valerate, and total VFA (P < 0.01) compared with conditioning for 15 or 45 min.
32910163	0	130	theme	corn	24:27	arg1	Effects					0:6	Effects	0:6	Effects of high-amylase corn on performance and carcass quality of finishing beef heifers.	0:89	Effects of high-amylase corn on performance and carcass quality of finishing beef heifers.
32910163	3	131	dep	in	618:619	arg1	vitro					621:625	vitro	621:625	vitro	621:625	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	1	132	from	use	115:117	arg1	production					135:144	fuel ethanol production	122:144	fuel ethanol production	122:144	Developed initially for use in fuel ethanol production, Enogen Feed Corn (EFC; Syngenta Crop Protection) is genetically modified to express high concentrations of α-amylase in the corn kernel.
32910163	2	133	theme	feedlot	393:399	arg1	performance					401:411	feedlot performance	393:411	feedlot performance	393:411	Experiments were conducted to evaluate processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence.
32910163	3	134	theme	dry	584:586	arg1	ISDMD					610:614	ISDMD	610:614	ISDMD	610:614	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	3	134	theme	dry	584:586	arg1	disappearance					595:607	in situ dry matter disappearance	576:607	in situ dry matter disappearance (ISDMD)	576:615	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	12	135	theme	P	1905:1905	arg1	treatments					1893:1902	treatments	1893:1902	treatments (P = 0.78)	1893:1913	Dry matter intakes were similar between treatments (P = 0.78), but cattle fed EFC had greater average daily gain (P < 0.01), improving feed efficiency by 5% (P < 0.01).
32910163	12	135	theme	P	1905:1905	arg1	0.78					1909:1912	P = 0.78	1905:1912	P = 0.78	1905:1912	Dry matter intakes were similar between treatments (P = 0.78), but cattle fed EFC had greater average daily gain (P < 0.01), improving feed efficiency by 5% (P < 0.01).
32910163	2	136	theme	effects	382:388	arg1	characteristics					334:348	processing characteristics	323:348	processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence	323:465	Experiments were conducted to evaluate processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence.
32910163	3	137	theme	in	576:577	arg1	ISDMD					610:614	ISDMD	610:614	ISDMD	610:614	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	3	137	theme	in	576:577	arg1	disappearance					595:607	in situ dry matter disappearance	576:607	in situ dry matter disappearance (ISDMD)	576:615	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	1	138	dep	EFC	165:167	arg1	Protection					184:193	Syngenta Crop Protection	170:193	EFC; Syngenta Crop Protection	165:193	Developed initially for use in fuel ethanol production, Enogen Feed Corn (EFC; Syngenta Crop Protection) is genetically modified to express high concentrations of α-amylase in the corn kernel.
32910163	17	139	used	used	2444:2447	arg2	corn					2432:2435	High-amylase corn	2419:2435	High-amylase corn	2419:2435	High-amylase corn may be used to improve microbial digestion, mill-throughput, and cattle performance, and it may mitigate liver abscesses.
32910163	0	140	theme	carcass	48:54	arg1	quality					56:62	carcass quality	48:62	carcass quality	48:62	Effects of high-amylase corn on performance and carcass quality of finishing beef heifers.
32910163	4	141	theme	%	816:816	arg1	EFC					832:834	0%, 25%, 50%, 75%, or 100% EFC	805:834	0%, 25%, 50%, 75%, or 100% EFC	805:834	Grains (EFC or mill-run control [CON]) were flaked to a density of 360 g/L, and mixtures with 0%, 25%, 50%, 75%, or 100% EFC were prepared.
32910163	12	142	theme	matter	1857:1862	arg1	intakes					1864:1870	Dry matter intakes	1853:1870	Dry matter intakes	1853:1870	Dry matter intakes were similar between treatments (P = 0.78), but cattle fed EFC had greater average daily gain (P < 0.01), improving feed efficiency by 5% (P < 0.01).
32910163	12	143	theme	P	1967:1967	arg1	<					1969:1969	P < 0.01	1967:1974	P < 0.01	1967:1974	Dry matter intakes were similar between treatments (P = 0.78), but cattle fed EFC had greater average daily gain (P < 0.01), improving feed efficiency by 5% (P < 0.01).
32910163	12	143	theme	P	1967:1967	arg1	gain					1961:1964	greater average daily gain	1939:1964	greater average daily gain (P < 0.01)	1939:1975	Dry matter intakes were similar between treatments (P = 0.78), but cattle fed EFC had greater average daily gain (P < 0.01), improving feed efficiency by 5% (P < 0.01).
32910163	9	144	theme	increasing	1424:1433	arg1	proportion					1435:1444	an increasing proportion	1421:1444	an increasing proportion of EFC	1421:1451	Starch availability, ISDMD, IVGP, acetate, propionate, valerate, and total VFA production increased with an increasing proportion of EFC (linear, P < 0.01).
32910163	14	145	theme	P	2218:2218	arg1	grade					2211:2215	USDA yield grade	2200:2215	USDA yield grade (P = 0.13)	2200:2226	No differences were observed for longissimus muscle area (P = 0.89), 12th-rib fat thickness (P = 0.21), or USDA yield grade (P = 0.13).
32910163	14	145	theme	P	2218:2218	arg1	0.13					2222:2225	P = 0.13	2218:2225	P = 0.13	2218:2225	No differences were observed for longissimus muscle area (P = 0.89), 12th-rib fat thickness (P = 0.21), or USDA yield grade (P = 0.13).
32910163	3	146	theme	randomized	488:497	arg1	design					514:519	a randomized complete block design	486:519	a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability	486:573	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	3	146	theme	randomized	488:497	arg1	factorial					532:540	3 × 3 × 5 factorial	522:540	3 × 3 × 5 factorial	522:540	Experiment 1 used a randomized complete block design (3 × 3 × 5 factorial) to evaluate starch availability, in situ dry matter disappearance (ISDMD), in vitro gas production (IVGP), and volatile fatty acid (VFA) profiles of in vitro cultures.
32910163	4	147	theme	%	830:830	arg1	EFC					832:834	0%, 25%, 50%, 75%, or 100% EFC	805:834	0%, 25%, 50%, 75%, or 100% EFC	805:834	Grains (EFC or mill-run control [CON]) were flaked to a density of 360 g/L, and mixtures with 0%, 25%, 50%, 75%, or 100% EFC were prepared.
32910163	12	148	theme	daily	1955:1959	arg1	<					1969:1969	P < 0.01	1967:1974	P < 0.01	1967:1974	Dry matter intakes were similar between treatments (P = 0.78), but cattle fed EFC had greater average daily gain (P < 0.01), improving feed efficiency by 5% (P < 0.01).
32910163	12	148	theme	daily	1955:1959	arg1	gain					1961:1964	greater average daily gain	1939:1964	greater average daily gain (P < 0.01)	1939:1975	Dry matter intakes were similar between treatments (P = 0.78), but cattle fed EFC had greater average daily gain (P < 0.01), improving feed efficiency by 5% (P < 0.01).
32910163	8	149	theme	valerate	1236:1243	arg1	production					1191:1200	production	1191:1200	production of acetate, propionate, butyrate, valerate, and total VFA (P < 0.01)	1191:1269	Steam conditioning for 30 min improved starch availability, IVGP, and production of acetate, propionate, butyrate, valerate, and total VFA (P < 0.01) compared with conditioning for 15 or 45 min.
32910163	8	149	theme	valerate	1236:1243	arg1	availability					1167:1178	starch availability	1160:1178	starch availability	1160:1178	Steam conditioning for 30 min improved starch availability, IVGP, and production of acetate, propionate, butyrate, valerate, and total VFA (P < 0.01) compared with conditioning for 15 or 45 min.
32910163	8	149	theme	valerate	1236:1243	arg1	IVGP					1181:1184	IVGP	1181:1184	IVGP	1181:1184	Steam conditioning for 30 min improved starch availability, IVGP, and production of acetate, propionate, butyrate, valerate, and total VFA (P < 0.01) compared with conditioning for 15 or 45 min.
32910163	2	150	theme	carcass	414:420	arg1	characteristics					422:436	carcass characteristics	414:436	carcass characteristics	414:436	Experiments were conducted to evaluate processing characteristics of EFC, in vitro digestion, and effects on feedlot performance, carcass characteristics, and liver abscess incidence.
32910163	10	151	used	used	1486:1489	arg2	Experiment					1473:1482	Experiment 2	1473:1484	Experiment 2	1473:1484	Experiment 2 used 700 beef heifers (394 ± 8.5 kg initial body weight [BW]) fed finishing diets with steam-flaked corn as CON or EFC for 136 d. Targeting similar starch availabilities, grains were processed to 360 g/L (CON) and 390 g/L for CON and EFC, respectively.
33799564	7	0	theme	expression	864:873	arg1	levels					875:880	Gene expression levels	859:880	Gene expression levels of IL-10 and polymeric immunoglobulin receptor (pIgR)	859:934	Gene expression levels of IL-10 and polymeric immunoglobulin receptor (pIgR) significantly increased in the HGB group.
33799564	1	1	theme	high-fat	215:222	arg1	mice					230:233	high-fat model mice	215:233	high-fat model mice	215:233	The prebiotic effect of high β-glucan barley (HGB) flour on the innate immune system of high-fat model mice was investigated.
33799564	5	2	theme	short-chain	682:692	arg1	SCFAs					707:711	SCFAs	707:711	SCFAs	707:711	Concentrations of short-chain fatty acids (SCFAs) and organic acids were analyzed by GC/MS.
33799564	5	2	theme	short-chain	682:692	arg1	acids					700:704	short-chain fatty acids	682:704	short-chain fatty acids (SCFAs)	682:712	Concentrations of short-chain fatty acids (SCFAs) and organic acids were analyzed by GC/MS.
33799564	4	3	theme	Real-time	456:464	arg1	PCR					466:468	Real-time PCR	456:468	Real-time PCR	456:468	Real-time PCR was used to determine mRNA expression levels of pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6 in the ileum as well as the composition of the microbiota in the cecum.
33799564	11	4	theme	immune	1562:1567	arg1	system					1569:1574	the immune system	1558:1574	the immune system	1558:1574	Moreover, we found that HGB flour modulated the gut microbiota, which increased the concentration of SCFAs, thereby stimulating the immune system.
33799564	1	5	theme	high	151:154	arg1	flour					178:182	high β-glucan barley (HGB) flour	151:182	high β-glucan barley (HGB) flour	151:182	The prebiotic effect of high β-glucan barley (HGB) flour on the innate immune system of high-fat model mice was investigated.
33799564	1	6	theme	model	224:228	arg1	mice					230:233	high-fat model mice	215:233	high-fat model mice	215:233	The prebiotic effect of high β-glucan barley (HGB) flour on the innate immune system of high-fat model mice was investigated.
33799564	0	7	theme	Obese	94:98	arg1	Mice					100:103	Diet-Induced Obese Mice	81:103	Diet-Induced Obese Mice	81:103	Ingestion of High β-Glucan Barley Flour Enhances the Intestinal Immune System of Diet-Induced Obese Mice by Prebiotic Effects.
33799564	9	8	theme	positive	1170:1177	arg1	correlation					1179:1189	a positive correlation	1168:1189	a positive correlation	1168:1189	Concentrations of propionate and lactate in the cecum were increased in the HGB group, and a positive correlation was found between these organic acids and the IL-10 expression level.
33799564	10	9	theme	high-fat	1415:1422	arg1	diet					1424:1427	a high-fat diet	1413:1427	a high-fat diet	1413:1427	Our findings showed that HGB flour enhanced immune function such as IgA secretion and IL-10 expression, even when the immune system was deteriorated by a high-fat diet.
33799564	2	10	theme	male	262:265	arg1	mice					267:270	C57BL/6J male mice	253:270	C57BL/6J male mice	253:270	C57BL/6J male mice were fed a high-fat diet supplemented with HGB flour for 90 days.
33799564	1	11	theme	mice	230:233	arg1	system					205:210	the innate immune system	187:210	the innate immune system of high-fat model mice	187:233	The prebiotic effect of high β-glucan barley (HGB) flour on the innate immune system of high-fat model mice was investigated.
33799564	0	12	theme	Diet-Induced	81:92	arg1	Mice					100:103	Diet-Induced Obese Mice	81:103	Diet-Induced Obese Mice	81:103	Ingestion of High β-Glucan Barley Flour Enhances the Intestinal Immune System of Diet-Induced Obese Mice by Prebiotic Effects.
33799564	10	13	theme	immune	1305:1310	arg1	function					1312:1319	immune function	1305:1319	immune function such as IgA secretion and IL-10 expression	1305:1362	Our findings showed that HGB flour enhanced immune function such as IgA secretion and IL-10 expression, even when the immune system was deteriorated by a high-fat diet.
33799564	2	14	theme	C57BL/6J	253:260	arg1	mice					267:270	C57BL/6J male mice	253:270	C57BL/6J male mice	253:270	C57BL/6J male mice were fed a high-fat diet supplemented with HGB flour for 90 days.
33799564	6	15	from	Concentrations	756:769	arg1	cecum					786:790	cecum	786:790	cecum	786:790	Concentrations of sIgA in the cecum and serum were increased in the HGB group compared to the control.
33799564	6	15	from	Concentrations	756:769	arg1	serum					796:800	serum	796:800	serum	796:800	Concentrations of sIgA in the cecum and serum were increased in the HGB group compared to the control.
33799564	11	16	theme	SCFAs	1531:1535	arg1	concentration					1514:1526	the concentration	1510:1526	the concentration of SCFAs	1510:1535	Moreover, we found that HGB flour modulated the gut microbiota, which increased the concentration of SCFAs, thereby stimulating the immune system.
33799564	4	17	used	used	474:477	arg2	PCR					466:468	Real-time PCR	456:468	Real-time PCR	456:468	Real-time PCR was used to determine mRNA expression levels of pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6 in the ileum as well as the composition of the microbiota in the cecum.
33799564	1	18	from	effect	141:146	arg1	system					205:210	the innate immune system	187:210	the innate immune system of high-fat model mice	187:233	The prebiotic effect of high β-glucan barley (HGB) flour on the innate immune system of high-fat model mice was investigated.
33799564	1	19	theme	β-glucan	156:163	arg1	flour					178:182	high β-glucan barley (HGB) flour	151:182	high β-glucan barley (HGB) flour	151:182	The prebiotic effect of high β-glucan barley (HGB) flour on the innate immune system of high-fat model mice was investigated.
33799564	0	20	theme	Mice	100:103	arg1	System					71:76	the Intestinal Immune System	49:76	the Intestinal Immune System of Diet-Induced Obese Mice	49:103	Ingestion of High β-Glucan Barley Flour Enhances the Intestinal Immune System of Diet-Induced Obese Mice by Prebiotic Effects.
33799564	6	21	dep	increased	807:815	arg1	compared					834:841	compared	834:841	compared to the control	834:856	Concentrations of sIgA in the cecum and serum were increased in the HGB group compared to the control.
33799564	4	22	theme	pro-	518:521	arg1	IL-6					587:590	IL-6	587:590	IL-6	587:590	Real-time PCR was used to determine mRNA expression levels of pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6 in the ileum as well as the composition of the microbiota in the cecum.
33799564	4	22	theme	pro-	518:521	arg1	IL					576:577	interleukin (IL)-10	563:581	interleukin (IL)-10	563:581	Real-time PCR was used to determine mRNA expression levels of pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6 in the ileum as well as the composition of the microbiota in the cecum.
33799564	4	22	theme	pro-	518:521	arg1	cytokines					545:553	pro- and anti-inflammatory cytokines	518:553	pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6	518:590	Real-time PCR was used to determine mRNA expression levels of pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6 in the ileum as well as the composition of the microbiota in the cecum.
33799564	9	23	theme	propionate	1095:1104	arg1	Concentrations					1077:1090	Concentrations	1077:1090	Concentrations of propionate and lactate in the cecum	1077:1129	Concentrations of propionate and lactate in the cecum were increased in the HGB group, and a positive correlation was found between these organic acids and the IL-10 expression level.
33799564	4	24	theme	cytokines	545:553	arg1	composition					620:630	the composition	616:630	mRNA expression levels of pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6 in the ileum as well as the composition of the microbiota in the cecum	492:661	Real-time PCR was used to determine mRNA expression levels of pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6 in the ileum as well as the composition of the microbiota in the cecum.
33799564	4	24	theme	cytokines	545:553	arg1	levels					508:513	mRNA expression levels	492:513	mRNA expression levels of pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6 in the ileum as well as the composition of the microbiota in the cecum	492:661	Real-time PCR was used to determine mRNA expression levels of pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6 in the ileum as well as the composition of the microbiota in the cecum.
33799564	1	25	theme	barley	165:170	arg1	flour					178:182	high β-glucan barley (HGB) flour	151:182	high β-glucan barley (HGB) flour	151:182	The prebiotic effect of high β-glucan barley (HGB) flour on the innate immune system of high-fat model mice was investigated.
33799564	3	26	theme	Secretory	338:346	arg1	sIgA					366:369	sIgA	366:369	sIgA	366:369	Secretory immunoglobulin A (sIgA) in the cecum and serum were analyzed by enzyme-linked immunosorbent assays (ELISA).
33799564	3	26	theme	Secretory	338:346	arg1	A					363:363	Secretory immunoglobulin A	338:363	Secretory immunoglobulin A (sIgA) in the cecum and serum	338:393	Secretory immunoglobulin A (sIgA) in the cecum and serum were analyzed by enzyme-linked immunosorbent assays (ELISA).
33799564	8	27	theme	microbiota	1022:1031	arg1	count					1013:1017	the bacterial count	999:1017	the bacterial count of microbiota	999:1031	HGB intake increased the bacterial count of microbiota, such as Bifidobacterium and Lactobacillus.
33799564	7	28	theme	HGB	967:969	arg1	group					971:975	the HGB group	963:975	the HGB group	963:975	Gene expression levels of IL-10 and polymeric immunoglobulin receptor (pIgR) significantly increased in the HGB group.
33799564	9	29	from	Concentrations	1077:1090	arg1	cecum					1125:1129	the cecum	1121:1129	the cecum	1121:1129	Concentrations of propionate and lactate in the cecum were increased in the HGB group, and a positive correlation was found between these organic acids and the IL-10 expression level.
33799564	3	30	theme	immunoglobulin	348:361	arg1	sIgA					366:369	sIgA	366:369	sIgA	366:369	Secretory immunoglobulin A (sIgA) in the cecum and serum were analyzed by enzyme-linked immunosorbent assays (ELISA).
33799564	3	30	theme	immunoglobulin	348:361	arg1	A					363:363	Secretory immunoglobulin A	338:363	Secretory immunoglobulin A (sIgA) in the cecum and serum	338:393	Secretory immunoglobulin A (sIgA) in the cecum and serum were analyzed by enzyme-linked immunosorbent assays (ELISA).
33799564	4	31	theme	mRNA	492:495	arg1	levels					508:513	mRNA expression levels	492:513	mRNA expression levels of pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6 in the ileum as well as the composition of the microbiota in the cecum	492:661	Real-time PCR was used to determine mRNA expression levels of pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6 in the ileum as well as the composition of the microbiota in the cecum.
33799564	10	32	theme	IgA	1329:1331	arg1	secretion					1333:1341	IgA secretion	1329:1341	IgA secretion	1329:1341	Our findings showed that HGB flour enhanced immune function such as IgA secretion and IL-10 expression, even when the immune system was deteriorated by a high-fat diet.
33799564	9	33	theme	expression	1243:1252	arg1	level					1254:1258	the IL-10 expression level	1233:1258	the IL-10 expression level	1233:1258	Concentrations of propionate and lactate in the cecum were increased in the HGB group, and a positive correlation was found between these organic acids and the IL-10 expression level.
33799564	4	34	theme	microbiota	639:648	arg1	composition					620:630	the composition	616:630	mRNA expression levels of pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6 in the ileum as well as the composition of the microbiota in the cecum	492:661	Real-time PCR was used to determine mRNA expression levels of pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6 in the ileum as well as the composition of the microbiota in the cecum.
33799564	4	34	theme	microbiota	639:648	arg1	levels					508:513	mRNA expression levels	492:513	mRNA expression levels of pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6 in the ileum as well as the composition of the microbiota in the cecum	492:661	Real-time PCR was used to determine mRNA expression levels of pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6 in the ileum as well as the composition of the microbiota in the cecum.
33799564	1	35	theme	HGB	173:175	arg1	flour					178:182	high β-glucan barley (HGB) flour	151:182	high β-glucan barley (HGB) flour	151:182	The prebiotic effect of high β-glucan barley (HGB) flour on the innate immune system of high-fat model mice was investigated.
33799564	0	36	theme	β-Glucan	18:25	arg1	Flour					34:38	High β-Glucan Barley Flour	13:38	High β-Glucan Barley Flour	13:38	Ingestion of High β-Glucan Barley Flour Enhances the Intestinal Immune System of Diet-Induced Obese Mice by Prebiotic Effects.
33799564	9	37	theme	lactate	1110:1116	arg1	Concentrations					1077:1090	Concentrations	1077:1090	Concentrations of propionate and lactate in the cecum	1077:1129	Concentrations of propionate and lactate in the cecum were increased in the HGB group, and a positive correlation was found between these organic acids and the IL-10 expression level.
33799564	10	38	theme	IL-10	1347:1351	arg1	expression					1353:1362	IL-10 expression	1347:1362	IL-10 expression	1347:1362	Our findings showed that HGB flour enhanced immune function such as IgA secretion and IL-10 expression, even when the immune system was deteriorated by a high-fat diet.
33799564	0	39	theme	High	13:16	arg1	Flour					34:38	High β-Glucan Barley Flour	13:38	High β-Glucan Barley Flour	13:38	Ingestion of High β-Glucan Barley Flour Enhances the Intestinal Immune System of Diet-Induced Obese Mice by Prebiotic Effects.
33799564	0	40	theme	Prebiotic	108:116	arg1	Effects					118:124	Prebiotic Effects	108:124	Prebiotic Effects	108:124	Ingestion of High β-Glucan Barley Flour Enhances the Intestinal Immune System of Diet-Induced Obese Mice by Prebiotic Effects.
33799564	6	41	theme	sIgA	774:777	arg1	Concentrations					756:769	Concentrations	756:769	Concentrations of sIgA in the cecum and serum	756:800	Concentrations of sIgA in the cecum and serum were increased in the HGB group compared to the control.
33799564	1	42	theme	flour	178:182	arg1	effect					141:146	The prebiotic effect	127:146	The prebiotic effect of high β-glucan barley (HGB) flour on the innate immune system of high-fat model mice	127:233	The prebiotic effect of high β-glucan barley (HGB) flour on the innate immune system of high-fat model mice was investigated.
33799564	0	43	theme	Flour	34:38	arg1	Ingestion					0:8	Ingestion	0:8	Ingestion of High β-Glucan Barley Flour	0:38	Ingestion of High β-Glucan Barley Flour Enhances the Intestinal Immune System of Diet-Induced Obese Mice by Prebiotic Effects.
33799564	4	44	theme	expression	497:506	arg1	levels					508:513	mRNA expression levels	492:513	mRNA expression levels of pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6 in the ileum as well as the composition of the microbiota in the cecum	492:661	Real-time PCR was used to determine mRNA expression levels of pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6 in the ileum as well as the composition of the microbiota in the cecum.
33799564	8	45	theme	HGB	978:980	arg1	intake					982:987	HGB intake	978:987	HGB intake	978:987	HGB intake increased the bacterial count of microbiota, such as Bifidobacterium and Lactobacillus.
33799564	0	46	theme	Barley	27:32	arg1	Flour					34:38	High β-Glucan Barley Flour	13:38	High β-Glucan Barley Flour	13:38	Ingestion of High β-Glucan Barley Flour Enhances the Intestinal Immune System of Diet-Induced Obese Mice by Prebiotic Effects.
33799564	5	47	theme	acids	700:704	arg1	Concentrations					664:677	Concentrations	664:677	Concentrations of short-chain fatty acids (SCFAs) and organic acids	664:730	Concentrations of short-chain fatty acids (SCFAs) and organic acids were analyzed by GC/MS.
33799564	5	48	theme	organic	718:724	arg1	acids					726:730	organic acids	718:730	organic acids	718:730	Concentrations of short-chain fatty acids (SCFAs) and organic acids were analyzed by GC/MS.
33799564	7	49	theme	polymeric	895:903	arg1	pIgR					930:933	pIgR	930:933	pIgR	930:933	Gene expression levels of IL-10 and polymeric immunoglobulin receptor (pIgR) significantly increased in the HGB group.
33799564	7	49	theme	polymeric	895:903	arg1	receptor					920:927	polymeric immunoglobulin receptor	895:927	polymeric immunoglobulin receptor (pIgR)	895:934	Gene expression levels of IL-10 and polymeric immunoglobulin receptor (pIgR) significantly increased in the HGB group.
33799564	11	50	theme	HGB	1454:1456	arg1	flour					1458:1462	HGB flour	1454:1462	HGB flour	1454:1462	Moreover, we found that HGB flour modulated the gut microbiota, which increased the concentration of SCFAs, thereby stimulating the immune system.
33799564	9	51	theme	IL-10	1237:1241	arg1	level					1254:1258	the IL-10 expression level	1233:1258	the IL-10 expression level	1233:1258	Concentrations of propionate and lactate in the cecum were increased in the HGB group, and a positive correlation was found between these organic acids and the IL-10 expression level.
33799564	3	52	link	enzyme-linked	412:424	arg1	assays					440:445	enzyme-linked immunosorbent assays	412:445	enzyme-linked immunosorbent assays (ELISA)	412:453	Secretory immunoglobulin A (sIgA) in the cecum and serum were analyzed by enzyme-linked immunosorbent assays (ELISA).
33799564	3	52	link	enzyme-linked	412:424	arg1	ELISA					448:452	ELISA	448:452	ELISA	448:452	Secretory immunoglobulin A (sIgA) in the cecum and serum were analyzed by enzyme-linked immunosorbent assays (ELISA).
33799564	9	53	theme	organic	1215:1221	arg1	acids					1223:1227	these organic acids	1209:1227	these organic acids	1209:1227	Concentrations of propionate and lactate in the cecum were increased in the HGB group, and a positive correlation was found between these organic acids and the IL-10 expression level.
33799564	3	54	dep	cecum	379:383	arg1	the					375:377	the	375:377	the	375:377	Secretory immunoglobulin A (sIgA) in the cecum and serum were analyzed by enzyme-linked immunosorbent assays (ELISA).
33799564	7	55	theme	immunoglobulin	905:918	arg1	pIgR					930:933	pIgR	930:933	pIgR	930:933	Gene expression levels of IL-10 and polymeric immunoglobulin receptor (pIgR) significantly increased in the HGB group.
33799564	7	55	theme	immunoglobulin	905:918	arg1	receptor					920:927	polymeric immunoglobulin receptor	895:927	polymeric immunoglobulin receptor (pIgR)	895:934	Gene expression levels of IL-10 and polymeric immunoglobulin receptor (pIgR) significantly increased in the HGB group.
33799564	6	56	theme	HGB	824:826	arg1	group					828:832	the HGB group	820:832	the HGB group	820:832	Concentrations of sIgA in the cecum and serum were increased in the HGB group compared to the control.
33799564	4	57	theme	anti-inflammatory	527:543	arg1	IL-6					587:590	IL-6	587:590	IL-6	587:590	Real-time PCR was used to determine mRNA expression levels of pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6 in the ileum as well as the composition of the microbiota in the cecum.
33799564	4	57	theme	anti-inflammatory	527:543	arg1	IL					576:577	interleukin (IL)-10	563:581	interleukin (IL)-10	563:581	Real-time PCR was used to determine mRNA expression levels of pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6 in the ileum as well as the composition of the microbiota in the cecum.
33799564	4	57	theme	anti-inflammatory	527:543	arg1	cytokines					545:553	pro- and anti-inflammatory cytokines	518:553	pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6	518:590	Real-time PCR was used to determine mRNA expression levels of pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6 in the ileum as well as the composition of the microbiota in the cecum.
33799564	10	58	theme	HGB	1286:1288	arg1	flour					1290:1294	HGB flour	1286:1294	HGB flour	1286:1294	Our findings showed that HGB flour enhanced immune function such as IgA secretion and IL-10 expression, even when the immune system was deteriorated by a high-fat diet.
33799564	7	59	theme	IL-10	885:889	arg1	levels					875:880	Gene expression levels	859:880	Gene expression levels of IL-10 and polymeric immunoglobulin receptor (pIgR)	859:934	Gene expression levels of IL-10 and polymeric immunoglobulin receptor (pIgR) significantly increased in the HGB group.
33799564	11	60	theme	gut	1478:1480	arg1	microbiota					1482:1491	the gut microbiota	1474:1491	the gut microbiota	1474:1491	Moreover, we found that HGB flour modulated the gut microbiota, which increased the concentration of SCFAs, thereby stimulating the immune system.
33799564	7	61	theme	receptor	920:927	arg1	levels					875:880	Gene expression levels	859:880	Gene expression levels of IL-10 and polymeric immunoglobulin receptor (pIgR)	859:934	Gene expression levels of IL-10 and polymeric immunoglobulin receptor (pIgR) significantly increased in the HGB group.
33799564	3	62	theme	enzyme-linked	412:424	arg1	assays					440:445	enzyme-linked immunosorbent assays	412:445	enzyme-linked immunosorbent assays (ELISA)	412:453	Secretory immunoglobulin A (sIgA) in the cecum and serum were analyzed by enzyme-linked immunosorbent assays (ELISA).
33799564	3	62	theme	enzyme-linked	412:424	arg1	ELISA					448:452	ELISA	448:452	ELISA	448:452	Secretory immunoglobulin A (sIgA) in the cecum and serum were analyzed by enzyme-linked immunosorbent assays (ELISA).
33799564	1	63	theme	innate	191:196	arg1	system					205:210	the innate immune system	187:210	the innate immune system of high-fat model mice	187:233	The prebiotic effect of high β-glucan barley (HGB) flour on the innate immune system of high-fat model mice was investigated.
33799564	5	64	theme	fatty	694:698	arg1	SCFAs					707:711	SCFAs	707:711	SCFAs	707:711	Concentrations of short-chain fatty acids (SCFAs) and organic acids were analyzed by GC/MS.
33799564	5	64	theme	fatty	694:698	arg1	acids					700:704	short-chain fatty acids	682:704	short-chain fatty acids (SCFAs)	682:712	Concentrations of short-chain fatty acids (SCFAs) and organic acids were analyzed by GC/MS.
33799564	9	65	theme	HGB	1153:1155	arg1	group					1157:1161	the HGB group	1149:1161	the HGB group	1149:1161	Concentrations of propionate and lactate in the cecum were increased in the HGB group, and a positive correlation was found between these organic acids and the IL-10 expression level.
33799564	3	66	dep	enzyme-linked	412:424	arg1	immunosorbent					426:438	immunosorbent	426:438	immunosorbent	426:438	Secretory immunoglobulin A (sIgA) in the cecum and serum were analyzed by enzyme-linked immunosorbent assays (ELISA).
33799564	8	67	theme	bacterial	1003:1011	arg1	count					1013:1017	the bacterial count	999:1017	the bacterial count of microbiota	999:1031	HGB intake increased the bacterial count of microbiota, such as Bifidobacterium and Lactobacillus.
33799564	2	68	theme	high-fat	283:290	arg1	diet					292:295	a high-fat diet	281:295	a high-fat diet supplemented with HGB flour for 90 days	281:335	C57BL/6J male mice were fed a high-fat diet supplemented with HGB flour for 90 days.
33799564	4	69	from	composition	620:630	arg1	ileum					599:603	the ileum	595:603	the ileum	595:603	Real-time PCR was used to determine mRNA expression levels of pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6 in the ileum as well as the composition of the microbiota in the cecum.
33799564	4	69	from	composition	620:630	arg1	cecum					657:661	the cecum	653:661	the cecum	653:661	Real-time PCR was used to determine mRNA expression levels of pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6 in the ileum as well as the composition of the microbiota in the cecum.
33799564	1	70	theme	immune	198:203	arg1	system					205:210	the innate immune system	187:210	the innate immune system of high-fat model mice	187:233	The prebiotic effect of high β-glucan barley (HGB) flour on the innate immune system of high-fat model mice was investigated.
33799564	0	71	theme	Immune	64:69	arg1	System					71:76	the Intestinal Immune System	49:76	the Intestinal Immune System of Diet-Induced Obese Mice	49:103	Ingestion of High β-Glucan Barley Flour Enhances the Intestinal Immune System of Diet-Induced Obese Mice by Prebiotic Effects.
33799564	10	72	theme	immune	1379:1384	arg1	system					1386:1391	the immune system	1375:1391	the immune system	1375:1391	Our findings showed that HGB flour enhanced immune function such as IgA secretion and IL-10 expression, even when the immune system was deteriorated by a high-fat diet.
33799564	1	73	theme	prebiotic	131:139	arg1	effect					141:146	The prebiotic effect	127:146	The prebiotic effect of high β-glucan barley (HGB) flour on the innate immune system of high-fat model mice	127:233	The prebiotic effect of high β-glucan barley (HGB) flour on the innate immune system of high-fat model mice was investigated.
33799564	4	74	theme	interleukin	563:573	arg1	IL					576:577	interleukin (IL)-10	563:581	interleukin (IL)-10	563:581	Real-time PCR was used to determine mRNA expression levels of pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6 in the ileum as well as the composition of the microbiota in the cecum.
33799564	0	75	theme	Intestinal	53:62	arg1	System					71:76	the Intestinal Immune System	49:76	the Intestinal Immune System of Diet-Induced Obese Mice	49:103	Ingestion of High β-Glucan Barley Flour Enhances the Intestinal Immune System of Diet-Induced Obese Mice by Prebiotic Effects.
33799564	3	76	from	A	363:363	arg1	cecum					379:383	cecum	379:383	cecum	379:383	Secretory immunoglobulin A (sIgA) in the cecum and serum were analyzed by enzyme-linked immunosorbent assays (ELISA).
33799564	3	76	from	A	363:363	arg1	serum					389:393	serum	389:393	serum	389:393	Secretory immunoglobulin A (sIgA) in the cecum and serum were analyzed by enzyme-linked immunosorbent assays (ELISA).
33799564	5	77	theme	acids	726:730	arg1	Concentrations					664:677	Concentrations	664:677	Concentrations of short-chain fatty acids (SCFAs) and organic acids	664:730	Concentrations of short-chain fatty acids (SCFAs) and organic acids were analyzed by GC/MS.
33799564	7	78	theme	Gene	859:862	arg1	levels					875:880	Gene expression levels	859:880	Gene expression levels of IL-10 and polymeric immunoglobulin receptor (pIgR)	859:934	Gene expression levels of IL-10 and polymeric immunoglobulin receptor (pIgR) significantly increased in the HGB group.
33799564	4	79	from	levels	508:513	arg1	ileum					599:603	the ileum	595:603	the ileum	595:603	Real-time PCR was used to determine mRNA expression levels of pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6 in the ileum as well as the composition of the microbiota in the cecum.
33799564	4	79	from	levels	508:513	arg1	cecum					657:661	the cecum	653:661	the cecum	653:661	Real-time PCR was used to determine mRNA expression levels of pro- and anti-inflammatory cytokines such as interleukin (IL)-10 and IL-6 in the ileum as well as the composition of the microbiota in the cecum.
33799564	6	80	dep	cecum	786:790	arg1	the					782:784	the	782:784	the	782:784	Concentrations of sIgA in the cecum and serum were increased in the HGB group compared to the control.
32882999	9	0	theme	related	1310:1316	arg1	benefits					1301:1308	potential benefits	1291:1308	potential benefits related to synbiotic effects and general gut health	1291:1360	These results provide evidence of potential benefits related to synbiotic effects and general gut health and support the potential of this food formulation as a therapeutic dietary intervention in mood and behavior disorders.
32882999	3	1	from	impact	433:438	arg1	composition					496:506	gut microbiota composition	481:506	gut microbiota composition	481:506	Here, we test the impact of a commercial synbiotic formulation on gut microbiota composition and metabolic activity.
32882999	3	1	from	impact	433:438	arg1	activity					522:529	metabolic activity	512:529	metabolic activity	512:529	Here, we test the impact of a commercial synbiotic formulation on gut microbiota composition and metabolic activity.
32882999	2	2	theme	normal	352:357	arg1	composition					370:380	normal microbiome composition	352:380	normal microbiome composition	352:380	Microbiota-targeting interventions are promising therapeutic modalities to restore or even maintain normal microbiome composition and activity in these disorders.
32882999	9	3	theme	dietary	1430:1436	arg1	intervention					1438:1449	a therapeutic dietary intervention	1416:1449	a therapeutic dietary intervention in mood and behavior disorders	1416:1480	These results provide evidence of potential benefits related to synbiotic effects and general gut health and support the potential of this food formulation as a therapeutic dietary intervention in mood and behavior disorders.
32882999	0	4	theme	Ingredient	69:78	arg1	Potential					28:36	the Prebiotic Potential	14:36	the Prebiotic Potential of a Commercial Synbiotic Food Ingredient	14:78	Evaluation of the Prebiotic Potential of a Commercial Synbiotic Food Ingredient on Gut Microbiota in an Ex Vivo Model of the Human Colon.
32882999	8	5	from	change	1226:1231	arg1	profiles					1247:1254	amino acid profiles	1236:1254	amino acid profiles	1236:1254	The test formulation did not lead to a significant change in amino acid profiles.
32882999	0	6	dep	Ex	104:105	arg1	Vivo					107:110	Vivo	107:110	Vivo	107:110	Evaluation of the Prebiotic Potential of a Commercial Synbiotic Food Ingredient on Gut Microbiota in an Ex Vivo Model of the Human Colon.
32882999	0	7	from	Evaluation	0:9	arg1	Microbiota					87:96	Gut Microbiota	83:96	Gut Microbiota in an Ex Vivo Model of the Human Colon	83:135	Evaluation of the Prebiotic Potential of a Commercial Synbiotic Food Ingredient on Gut Microbiota in an Ex Vivo Model of the Human Colon.
32882999	7	8	theme	microcrystalline	1123:1138	arg1	cellulose					1140:1148	microcrystalline cellulose	1123:1148	microcrystalline cellulose	1123:1148	The test formulation significantly increased the level of microbiota-generated butyrate within 12 h of treatment as compared to 24 h required by microcrystalline cellulose to boost its production.
32882999	7	9	theme	test	982:985	arg1	formulation					987:997	The test formulation	978:997	The test formulation	978:997	The test formulation significantly increased the level of microbiota-generated butyrate within 12 h of treatment as compared to 24 h required by microcrystalline cellulose to boost its production.
32882999	4	10	theme	fiber	782:786	arg1	control					788:794	a dietary fiber control	772:794	a dietary fiber control	772:794	We employed an ex-vivo continuous fermentation model that simulates the proximal colon to assess the effect of this formulation on microbiota structure and functionality as compared to no treatment control and microcrystalline cellulose as a dietary fiber control.
32882999	1	11	theme	microbiota-gut-brain	224:243	arg1	"					249:249	the "microbiota-gut-brain axis"	219:249	the "microbiota-gut-brain axis"	219:249	Behavior and mood disorders have been linked to gut microbiota dysbiosis through the "microbiota-gut-brain axis".
32882999	0	12	theme	Gut	83:85	arg1	Microbiota					87:96	Gut Microbiota	83:96	Gut Microbiota in an Ex Vivo Model of the Human Colon	83:135	Evaluation of the Prebiotic Potential of a Commercial Synbiotic Food Ingredient on Gut Microbiota in an Ex Vivo Model of the Human Colon.
32882999	4	13	theme	dietary	774:780	arg1	control					788:794	a dietary fiber control	772:794	a dietary fiber control	772:794	We employed an ex-vivo continuous fermentation model that simulates the proximal colon to assess the effect of this formulation on microbiota structure and functionality as compared to no treatment control and microcrystalline cellulose as a dietary fiber control.
32882999	2	14	theme	Microbiota-targeting	252:271	arg1	interventions					273:285	Microbiota-targeting interventions	252:285	Microbiota-targeting interventions	252:285	Microbiota-targeting interventions are promising therapeutic modalities to restore or even maintain normal microbiome composition and activity in these disorders.
32882999	2	14	theme	Microbiota-targeting	252:271	arg1	modalities					313:322	promising therapeutic modalities	291:322	promising therapeutic modalities	291:322	Microbiota-targeting interventions are promising therapeutic modalities to restore or even maintain normal microbiome composition and activity in these disorders.
32882999	1	15	theme	axis	245:248	arg1	"					249:249	the "microbiota-gut-brain axis"	219:249	the "microbiota-gut-brain axis"	219:249	Behavior and mood disorders have been linked to gut microbiota dysbiosis through the "microbiota-gut-brain axis".
32882999	5	16	theme	gut	849:851	arg1	microbiota					853:862	gut microbiota	849:862	gut microbiota	849:862	The test formulation did not alter the diversity of gut microbiota over 48 h of treatment.
32882999	9	17	theme	general	1343:1349	arg1	health					1355:1360	general gut health	1343:1360	general gut health	1343:1360	These results provide evidence of potential benefits related to synbiotic effects and general gut health and support the potential of this food formulation as a therapeutic dietary intervention in mood and behavior disorders.
32882999	4	18	theme	microbiota	663:672	arg1	structure					674:682	microbiota structure	663:682	microbiota structure	663:682	We employed an ex-vivo continuous fermentation model that simulates the proximal colon to assess the effect of this formulation on microbiota structure and functionality as compared to no treatment control and microcrystalline cellulose as a dietary fiber control.
32882999	9	19	theme	synbiotic	1321:1329	arg1	effects					1331:1337	synbiotic effects	1321:1337	synbiotic effects	1321:1337	These results provide evidence of potential benefits related to synbiotic effects and general gut health and support the potential of this food formulation as a therapeutic dietary intervention in mood and behavior disorders.
32882999	9	20	theme	formulation	1401:1411	arg1	potential					1378:1386	the potential	1374:1386	the potential of this food formulation as a therapeutic dietary intervention in mood and behavior disorders	1374:1480	These results provide evidence of potential benefits related to synbiotic effects and general gut health and support the potential of this food formulation as a therapeutic dietary intervention in mood and behavior disorders.
32882999	6	21	theme	Erysipelotrichaceae	957:975	arg1	enrichment					912:921	the enrichment	908:921	the enrichment of Lactobacillus, Collinsella and Erysipelotrichaceae	908:975	However, it induced the enrichment of Lactobacillus, Collinsella and Erysipelotrichaceae.
32882999	1	22	theme	mood	151:154	arg1	disorders					156:164	mood disorders	151:164	mood disorders	151:164	Behavior and mood disorders have been linked to gut microbiota dysbiosis through the "microbiota-gut-brain axis".
32882999	9	23	theme	gut	1351:1353	arg1	health					1355:1360	general gut health	1343:1360	general gut health	1343:1360	These results provide evidence of potential benefits related to synbiotic effects and general gut health and support the potential of this food formulation as a therapeutic dietary intervention in mood and behavior disorders.
32882999	4	24	theme	continuous	555:564	arg1	model					579:583	an ex-vivo continuous fermentation model	544:583	an ex-vivo continuous fermentation model that simulates the proximal colon to assess the effect of this formulation on microbiota structure and functionality as compared to no treatment control and microcrystalline cellulose as a dietary fiber control	544:794	We employed an ex-vivo continuous fermentation model that simulates the proximal colon to assess the effect of this formulation on microbiota structure and functionality as compared to no treatment control and microcrystalline cellulose as a dietary fiber control.
32882999	9	25	theme	potential	1291:1299	arg1	benefits					1301:1308	potential benefits	1291:1308	potential benefits related to synbiotic effects and general gut health	1291:1360	These results provide evidence of potential benefits related to synbiotic effects and general gut health and support the potential of this food formulation as a therapeutic dietary intervention in mood and behavior disorders.
32882999	2	26	theme	therapeutic	301:311	arg1	interventions					273:285	Microbiota-targeting interventions	252:285	Microbiota-targeting interventions	252:285	Microbiota-targeting interventions are promising therapeutic modalities to restore or even maintain normal microbiome composition and activity in these disorders.
32882999	2	26	theme	therapeutic	301:311	arg1	modalities					313:322	promising therapeutic modalities	291:322	promising therapeutic modalities	291:322	Microbiota-targeting interventions are promising therapeutic modalities to restore or even maintain normal microbiome composition and activity in these disorders.
32882999	5	27	theme	treatment	877:885	arg1	h					872:872	48 h	869:872	48 h of treatment	869:885	The test formulation did not alter the diversity of gut microbiota over 48 h of treatment.
32882999	0	28	theme	Prebiotic	18:26	arg1	Potential					28:36	the Prebiotic Potential	14:36	the Prebiotic Potential of a Commercial Synbiotic Food Ingredient	14:78	Evaluation of the Prebiotic Potential of a Commercial Synbiotic Food Ingredient on Gut Microbiota in an Ex Vivo Model of the Human Colon.
32882999	4	29	theme	ex-vivo	547:553	arg1	model					579:583	an ex-vivo continuous fermentation model	544:583	an ex-vivo continuous fermentation model that simulates the proximal colon to assess the effect of this formulation on microbiota structure and functionality as compared to no treatment control and microcrystalline cellulose as a dietary fiber control	544:794	We employed an ex-vivo continuous fermentation model that simulates the proximal colon to assess the effect of this formulation on microbiota structure and functionality as compared to no treatment control and microcrystalline cellulose as a dietary fiber control.
32882999	2	30	theme	promising	291:299	arg1	interventions					273:285	Microbiota-targeting interventions	252:285	Microbiota-targeting interventions	252:285	Microbiota-targeting interventions are promising therapeutic modalities to restore or even maintain normal microbiome composition and activity in these disorders.
32882999	2	30	theme	promising	291:299	arg1	modalities					313:322	promising therapeutic modalities	291:322	promising therapeutic modalities	291:322	Microbiota-targeting interventions are promising therapeutic modalities to restore or even maintain normal microbiome composition and activity in these disorders.
32882999	3	31	theme	commercial	445:454	arg1	formulation					466:476	a commercial synbiotic formulation	443:476	a commercial synbiotic formulation	443:476	Here, we test the impact of a commercial synbiotic formulation on gut microbiota composition and metabolic activity.
32882999	4	32	theme	microcrystalline	742:757	arg1	cellulose					759:767	microcrystalline cellulose	742:767	microcrystalline cellulose	742:767	We employed an ex-vivo continuous fermentation model that simulates the proximal colon to assess the effect of this formulation on microbiota structure and functionality as compared to no treatment control and microcrystalline cellulose as a dietary fiber control.
32882999	0	33	theme	Ex	104:105	arg1	Model					112:116	an Ex Vivo Model	101:116	an Ex Vivo Model of the Human Colon	101:135	Evaluation of the Prebiotic Potential of a Commercial Synbiotic Food Ingredient on Gut Microbiota in an Ex Vivo Model of the Human Colon.
32882999	9	34	from	intervention	1438:1449	arg1	behavior					1463:1470	behavior	1463:1470	behavior	1463:1470	These results provide evidence of potential benefits related to synbiotic effects and general gut health and support the potential of this food formulation as a therapeutic dietary intervention in mood and behavior disorders.
32882999	9	34	from	intervention	1438:1449	arg1	mood					1454:1457	mood	1454:1457	mood	1454:1457	These results provide evidence of potential benefits related to synbiotic effects and general gut health and support the potential of this food formulation as a therapeutic dietary intervention in mood and behavior disorders.
32882999	3	35	theme	synbiotic	456:464	arg1	formulation					466:476	a commercial synbiotic formulation	443:476	a commercial synbiotic formulation	443:476	Here, we test the impact of a commercial synbiotic formulation on gut microbiota composition and metabolic activity.
32882999	4	36	theme	fermentation	566:577	arg1	model					579:583	an ex-vivo continuous fermentation model	544:583	an ex-vivo continuous fermentation model that simulates the proximal colon to assess the effect of this formulation on microbiota structure and functionality as compared to no treatment control and microcrystalline cellulose as a dietary fiber control	544:794	We employed an ex-vivo continuous fermentation model that simulates the proximal colon to assess the effect of this formulation on microbiota structure and functionality as compared to no treatment control and microcrystalline cellulose as a dietary fiber control.
32882999	7	37	theme	treatment	1081:1089	arg1	h					1076:1076	12 h	1073:1076	12 h of treatment	1073:1089	The test formulation significantly increased the level of microbiota-generated butyrate within 12 h of treatment as compared to 24 h required by microcrystalline cellulose to boost its production.
32882999	5	38	theme	microbiota	853:862	arg1	diversity					836:844	the diversity	832:844	the diversity of gut microbiota	832:862	The test formulation did not alter the diversity of gut microbiota over 48 h of treatment.
32882999	4	39	from	effect	633:638	arg1	functionality					688:700	functionality	688:700	functionality	688:700	We employed an ex-vivo continuous fermentation model that simulates the proximal colon to assess the effect of this formulation on microbiota structure and functionality as compared to no treatment control and microcrystalline cellulose as a dietary fiber control.
32882999	4	39	from	effect	633:638	arg1	structure					674:682	microbiota structure	663:682	microbiota structure	663:682	We employed an ex-vivo continuous fermentation model that simulates the proximal colon to assess the effect of this formulation on microbiota structure and functionality as compared to no treatment control and microcrystalline cellulose as a dietary fiber control.
32882999	4	40	theme	formulation	648:658	arg1	effect					633:638	the effect	629:638	the effect of this formulation on microbiota structure and functionality	629:700	We employed an ex-vivo continuous fermentation model that simulates the proximal colon to assess the effect of this formulation on microbiota structure and functionality as compared to no treatment control and microcrystalline cellulose as a dietary fiber control.
32882999	0	41	theme	Potential	28:36	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of the Prebiotic Potential of a Commercial Synbiotic Food Ingredient on Gut Microbiota in an Ex Vivo Model of the Human Colon.	0:136	Evaluation of the Prebiotic Potential of a Commercial Synbiotic Food Ingredient on Gut Microbiota in an Ex Vivo Model of the Human Colon.
32882999	5	42	theme	test	801:804	arg1	formulation					806:816	The test formulation	797:816	The test formulation	797:816	The test formulation did not alter the diversity of gut microbiota over 48 h of treatment.
32882999	8	43	theme	significant	1214:1224	arg1	change					1226:1231	a significant change	1212:1231	a significant change in amino acid profiles	1212:1254	The test formulation did not lead to a significant change in amino acid profiles.
32882999	7	44	theme	microbiota-generated	1036:1055	arg1	butyrate					1057:1064	microbiota-generated butyrate	1036:1064	microbiota-generated butyrate	1036:1064	The test formulation significantly increased the level of microbiota-generated butyrate within 12 h of treatment as compared to 24 h required by microcrystalline cellulose to boost its production.
32882999	9	45	theme	food	1396:1399	arg1	formulation					1401:1411	this food formulation	1391:1411	this food formulation	1391:1411	These results provide evidence of potential benefits related to synbiotic effects and general gut health and support the potential of this food formulation as a therapeutic dietary intervention in mood and behavior disorders.
32882999	0	46	theme	Commercial	43:52	arg1	Ingredient					69:78	a Commercial Synbiotic Food Ingredient	41:78	a Commercial Synbiotic Food Ingredient	41:78	Evaluation of the Prebiotic Potential of a Commercial Synbiotic Food Ingredient on Gut Microbiota in an Ex Vivo Model of the Human Colon.
32882999	6	47	theme	Collinsella	941:951	arg1	enrichment					912:921	the enrichment	908:921	the enrichment of Lactobacillus, Collinsella and Erysipelotrichaceae	908:975	However, it induced the enrichment of Lactobacillus, Collinsella and Erysipelotrichaceae.
32882999	0	48	theme	Human	125:129	arg1	Colon					131:135	the Human Colon	121:135	the Human Colon	121:135	Evaluation of the Prebiotic Potential of a Commercial Synbiotic Food Ingredient on Gut Microbiota in an Ex Vivo Model of the Human Colon.
32882999	7	49	theme	butyrate	1057:1064	arg1	level					1027:1031	the level	1023:1031	the level of microbiota-generated butyrate	1023:1064	The test formulation significantly increased the level of microbiota-generated butyrate within 12 h of treatment as compared to 24 h required by microcrystalline cellulose to boost its production.
32882999	3	50	theme	gut	481:483	arg1	composition					496:506	gut microbiota composition	481:506	gut microbiota composition	481:506	Here, we test the impact of a commercial synbiotic formulation on gut microbiota composition and metabolic activity.
32882999	8	51	theme	amino	1236:1240	arg1	profiles					1247:1254	amino acid profiles	1236:1254	amino acid profiles	1236:1254	The test formulation did not lead to a significant change in amino acid profiles.
32882999	4	52	theme	proximal	604:611	arg1	colon					613:617	the proximal colon	600:617	the proximal colon	600:617	We employed an ex-vivo continuous fermentation model that simulates the proximal colon to assess the effect of this formulation on microbiota structure and functionality as compared to no treatment control and microcrystalline cellulose as a dietary fiber control.
32882999	3	53	theme	microbiota	485:494	arg1	composition					496:506	gut microbiota composition	481:506	gut microbiota composition	481:506	Here, we test the impact of a commercial synbiotic formulation on gut microbiota composition and metabolic activity.
32882999	6	54	theme	Lactobacillus	926:938	arg1	enrichment					912:921	the enrichment	908:921	the enrichment of Lactobacillus, Collinsella and Erysipelotrichaceae	908:975	However, it induced the enrichment of Lactobacillus, Collinsella and Erysipelotrichaceae.
32882999	9	55	dep	mood	1454:1457	arg1	disorders					1472:1480	disorders	1472:1480	disorders	1472:1480	These results provide evidence of potential benefits related to synbiotic effects and general gut health and support the potential of this food formulation as a therapeutic dietary intervention in mood and behavior disorders.
32882999	3	56	theme	formulation	466:476	arg1	impact					433:438	the impact	429:438	the impact of a commercial synbiotic formulation on gut microbiota composition and metabolic activity	429:529	Here, we test the impact of a commercial synbiotic formulation on gut microbiota composition and metabolic activity.
32882999	4	57	dep	treatment	720:728	arg1	control					730:736	control	730:736	control	730:736	We employed an ex-vivo continuous fermentation model that simulates the proximal colon to assess the effect of this formulation on microbiota structure and functionality as compared to no treatment control and microcrystalline cellulose as a dietary fiber control.
32882999	1	58	theme	gut	186:188	arg1	dysbiosis					201:209	gut microbiota dysbiosis	186:209	gut microbiota dysbiosis	186:209	Behavior and mood disorders have been linked to gut microbiota dysbiosis through the "microbiota-gut-brain axis".
32882999	0	59	theme	Colon	131:135	arg1	Model					112:116	an Ex Vivo Model	101:116	an Ex Vivo Model of the Human Colon	101:135	Evaluation of the Prebiotic Potential of a Commercial Synbiotic Food Ingredient on Gut Microbiota in an Ex Vivo Model of the Human Colon.
32882999	0	60	from	Microbiota	87:96	arg1	Model					112:116	an Ex Vivo Model	101:116	an Ex Vivo Model of the Human Colon	101:135	Evaluation of the Prebiotic Potential of a Commercial Synbiotic Food Ingredient on Gut Microbiota in an Ex Vivo Model of the Human Colon.
32882999	8	61	theme	acid	1242:1245	arg1	profiles					1247:1254	amino acid profiles	1236:1254	amino acid profiles	1236:1254	The test formulation did not lead to a significant change in amino acid profiles.
32882999	1	62	theme	microbiota	190:199	arg1	dysbiosis					201:209	gut microbiota dysbiosis	186:209	gut microbiota dysbiosis	186:209	Behavior and mood disorders have been linked to gut microbiota dysbiosis through the "microbiota-gut-brain axis".
32882999	0	63	theme	Food	64:67	arg1	Ingredient					69:78	a Commercial Synbiotic Food Ingredient	41:78	a Commercial Synbiotic Food Ingredient	41:78	Evaluation of the Prebiotic Potential of a Commercial Synbiotic Food Ingredient on Gut Microbiota in an Ex Vivo Model of the Human Colon.
32882999	1	64	attach	linked	176:181	arg1	dysbiosis					201:209	gut microbiota dysbiosis	186:209	gut microbiota dysbiosis	186:209	Behavior and mood disorders have been linked to gut microbiota dysbiosis through the "microbiota-gut-brain axis".
32882999	1	64	attach	linked	176:181	arg2	Behavior					138:145	Behavior	138:145	Behavior	138:145	Behavior and mood disorders have been linked to gut microbiota dysbiosis through the "microbiota-gut-brain axis".
32882999	1	64	attach	linked	176:181	arg2	disorders					156:164	mood disorders	151:164	mood disorders	151:164	Behavior and mood disorders have been linked to gut microbiota dysbiosis through the "microbiota-gut-brain axis".
32882999	3	65	theme	metabolic	512:520	arg1	activity					522:529	metabolic activity	512:529	metabolic activity	512:529	Here, we test the impact of a commercial synbiotic formulation on gut microbiota composition and metabolic activity.
32882999	0	66	theme	Synbiotic	54:62	arg1	Ingredient					69:78	a Commercial Synbiotic Food Ingredient	41:78	a Commercial Synbiotic Food Ingredient	41:78	Evaluation of the Prebiotic Potential of a Commercial Synbiotic Food Ingredient on Gut Microbiota in an Ex Vivo Model of the Human Colon.
32882999	9	67	theme	benefits	1301:1308	arg1	evidence					1279:1286	evidence	1279:1286	evidence of potential benefits related to synbiotic effects and general gut health	1279:1360	These results provide evidence of potential benefits related to synbiotic effects and general gut health and support the potential of this food formulation as a therapeutic dietary intervention in mood and behavior disorders.
32882999	9	68	theme	therapeutic	1418:1428	arg1	intervention					1438:1449	a therapeutic dietary intervention	1416:1449	a therapeutic dietary intervention in mood and behavior disorders	1416:1480	These results provide evidence of potential benefits related to synbiotic effects and general gut health and support the potential of this food formulation as a therapeutic dietary intervention in mood and behavior disorders.
32882999	2	69	theme	microbiome	359:368	arg1	composition					370:380	normal microbiome composition	352:380	normal microbiome composition	352:380	Microbiota-targeting interventions are promising therapeutic modalities to restore or even maintain normal microbiome composition and activity in these disorders.
32882999	8	70	theme	test	1179:1182	arg1	formulation					1184:1194	The test formulation	1175:1194	The test formulation	1175:1194	The test formulation did not lead to a significant change in amino acid profiles.
33363672	11	0	theme	glycopeptide	1880:1891	arg1	identification					1893:1906	MS/MS- and MS1-based glycopeptide identification	1859:1906	MS/MS- and MS1-based glycopeptide identification	1859:1906	Finally, this study presents a semiautomated workflow for reliable glycoproteomic data analysis by the combination of software packages for MS/MS- and MS1-based glycopeptide identification as well as the integration of analyte quality control and quantification.
33363672	11	1	theme	reliable	1777:1784	arg1	analysis					1806:1813	reliable glycoproteomic data analysis	1777:1813	reliable glycoproteomic data analysis by the combination of software packages	1777:1853	Finally, this study presents a semiautomated workflow for reliable glycoproteomic data analysis by the combination of software packages for MS/MS- and MS1-based glycopeptide identification as well as the integration of analyte quality control and quantification.
33363672	2	2	theme	reliable	378:385	arg1	tools					401:405	reliable data analysis tools	378:405	reliable data analysis tools	378:405	The use of glycopeptide-centered glycoproteomics by mass spectrometry is rapidly evolving in many research areas, leading to a demand in reliable data analysis tools.
33363672	6	3	theme	glycopeptide	1017:1028	arg1	identification					1030:1043	Initial glycopeptide identification	1009:1043	Initial glycopeptide identification based on MS/MS data	1009:1063	Initial glycopeptide identification based on MS/MS data was aided by the Byonic software.
33363672	11	4	theme	MS/MS-	1859:1864	arg1	identification					1893:1906	MS/MS- and MS1-based glycopeptide identification	1859:1906	MS/MS- and MS1-based glycopeptide identification	1859:1906	Finally, this study presents a semiautomated workflow for reliable glycoproteomic data analysis by the combination of software packages for MS/MS- and MS1-based glycopeptide identification as well as the integration of analyte quality control and quantification.
33363672	7	5	theme	annotated	1275:1283	arg1	glycopeptides					1285:1297	confidently annotated glycopeptides	1263:1297	confidently annotated glycopeptides	1263:1297	Additional MS1-based glycopeptide identification relying on accurate mass and retention time differences using GlycopeptideGraphMS considerably expanded the set of confidently annotated glycopeptides.
33363672	3	6	theme	glycopeptides	540:552	arg1	quantification					522:535	quantification	522:535	quantification	522:535	In recent years, several bioinformatic tools were developed to facilitate and improve both the identification and quantification of glycopeptides.
33363672	3	6	theme	glycopeptides	540:552	arg1	identification					503:516	identification	503:516	identification	503:516	In recent years, several bioinformatic tools were developed to facilitate and improve both the identification and quantification of glycopeptides.
33363672	8	7	theme	LaCyTools	1352:1360	arg1	performance					1337:1347	the performance	1333:1347	the performance of LaCyTools	1333:1360	For glycopeptide quantification, the performance of LaCyTools was compared to Skyline, and GlycopeptideGraphMS.
33363672	2	8	from	demand	368:373	arg1	tools					401:405	reliable data analysis tools	378:405	reliable data analysis tools	378:405	The use of glycopeptide-centered glycoproteomics by mass spectrometry is rapidly evolving in many research areas, leading to a demand in reliable data analysis tools.
33363672	4	9	theme	targeting	704:712	arg1	glycoproteins					723:735	workflow targeting enriched glycoproteins	695:735	workflow targeting enriched glycoproteins	695:735	Here, a selection of these tools was combined and evaluated with the aim of establishing a robust glycopeptide detection and quantification workflow targeting enriched glycoproteins.
33363672	5	10	theme	mass	887:890	arg1	spectrometry					892:903	a nano-reversed-phase liquid chromatography-tandem mass spectrometry	836:903	a nano-reversed-phase liquid chromatography-tandem mass spectrometry platform with a high-resolution mass analyzer and higher-energy collisional dissociation fragmentation	836:1006	For this purpose, a tryptic digest from affinity-purified immunoglobulins G and A was analyzed on a nano-reversed-phase liquid chromatography-tandem mass spectrometry platform with a high-resolution mass analyzer and higher-energy collisional dissociation fragmentation.
33363672	10	11	theme	peptide	1630:1636	arg1	oxidation					1577:1585	Partial cysteine oxidation	1560:1585	Partial cysteine oxidation	1560:1585	Partial cysteine oxidation was identified as an unexpectedly abundant peptide modification and impaired the automated processing of several IgA glycopeptides.
33363672	10	11	theme	peptide	1630:1636	arg1	processing					1678:1687	the automated processing	1664:1687	the automated processing of several IgA glycopeptides	1664:1716	Partial cysteine oxidation was identified as an unexpectedly abundant peptide modification and impaired the automated processing of several IgA glycopeptides.
33363672	10	11	theme	peptide	1630:1636	arg1	modification					1638:1649	an unexpectedly abundant peptide modification	1605:1649	an unexpectedly abundant peptide modification	1605:1649	Partial cysteine oxidation was identified as an unexpectedly abundant peptide modification and impaired the automated processing of several IgA glycopeptides.
33363672	5	12	theme	collisional	969:979	arg1	fragmentation					994:1006	higher-energy collisional dissociation fragmentation	955:1006	higher-energy collisional dissociation fragmentation	955:1006	For this purpose, a tryptic digest from affinity-purified immunoglobulins G and A was analyzed on a nano-reversed-phase liquid chromatography-tandem mass spectrometry platform with a high-resolution mass analyzer and higher-energy collisional dissociation fragmentation.
33363672	6	13	theme	Byonic	1082:1087	arg1	software					1089:1096	the Byonic software	1078:1096	the Byonic software	1078:1096	Initial glycopeptide identification based on MS/MS data was aided by the Byonic software.
33363672	5	14	from	immunoglobulins	796:810	arg1	digest					766:771	a tryptic digest	756:771	a tryptic digest from affinity-purified immunoglobulins G and A	756:818	For this purpose, a tryptic digest from affinity-purified immunoglobulins G and A was analyzed on a nano-reversed-phase liquid chromatography-tandem mass spectrometry platform with a high-resolution mass analyzer and higher-energy collisional dissociation fragmentation.
33363672	2	15	theme	research	339:346	arg1	areas					348:352	many research areas	334:352	many research areas	334:352	The use of glycopeptide-centered glycoproteomics by mass spectrometry is rapidly evolving in many research areas, leading to a demand in reliable data analysis tools.
33363672	1	16	theme	portions	231:238	arg1	diversity					199:207	the high structural diversity	179:207	the high structural diversity of peptide and glycan portions	179:238	Glycoproteomic data are often very complex, reflecting the high structural diversity of peptide and glycan portions.
33363672	9	17	from	differences	1499:1509	arg1	terms					1514:1518	terms	1514:1518	terms of robustness and data quality control	1514:1557	All quantification packages resulted in comparable glycosylation profiles but featured differences in terms of robustness and data quality control.
33363672	2	18	theme	glycopeptide-centered	252:272	arg1	glycoproteomics					274:288	glycopeptide-centered glycoproteomics	252:288	glycopeptide-centered glycoproteomics	252:288	The use of glycopeptide-centered glycoproteomics by mass spectrometry is rapidly evolving in many research areas, leading to a demand in reliable data analysis tools.
33363672	5	19	theme	mass	937:940	arg1	analyzer					942:949	a high-resolution mass analyzer	919:949	a high-resolution mass analyzer	919:949	For this purpose, a tryptic digest from affinity-purified immunoglobulins G and A was analyzed on a nano-reversed-phase liquid chromatography-tandem mass spectrometry platform with a high-resolution mass analyzer and higher-energy collisional dissociation fragmentation.
33363672	5	20	dep	immunoglobulins	796:810	arg1	immunoglobulins					796:810	affinity-purified immunoglobulins G and A	778:818	affinity-purified immunoglobulins G and A	778:818	For this purpose, a tryptic digest from affinity-purified immunoglobulins G and A was analyzed on a nano-reversed-phase liquid chromatography-tandem mass spectrometry platform with a high-resolution mass analyzer and higher-energy collisional dissociation fragmentation.
33363672	5	20	dep	immunoglobulins	796:810	arg1	A					818:818	A	818:818	A	818:818	For this purpose, a tryptic digest from affinity-purified immunoglobulins G and A was analyzed on a nano-reversed-phase liquid chromatography-tandem mass spectrometry platform with a high-resolution mass analyzer and higher-energy collisional dissociation fragmentation.
33363672	5	20	dep	immunoglobulins	796:810	arg1	G					812:812	G	812:812	G	812:812	For this purpose, a tryptic digest from affinity-purified immunoglobulins G and A was analyzed on a nano-reversed-phase liquid chromatography-tandem mass spectrometry platform with a high-resolution mass analyzer and higher-energy collisional dissociation fragmentation.
33363672	7	21	theme	time	1187:1190	arg1	differences					1192:1202	accurate mass and retention time differences	1159:1202	accurate mass and retention time differences using GlycopeptideGraphMS	1159:1228	Additional MS1-based glycopeptide identification relying on accurate mass and retention time differences using GlycopeptideGraphMS considerably expanded the set of confidently annotated glycopeptides.
33363672	7	22	theme	identification	1133:1146	arg1	relying					1148:1154	Additional MS1-based glycopeptide identification relying	1099:1154	Additional MS1-based glycopeptide identification relying on accurate mass and retention time differences using GlycopeptideGraphMS	1099:1228	Additional MS1-based glycopeptide identification relying on accurate mass and retention time differences using GlycopeptideGraphMS considerably expanded the set of confidently annotated glycopeptides.
33363672	2	23	from	evolving	322:329	arg1	areas					348:352	many research areas	334:352	many research areas	334:352	The use of glycopeptide-centered glycoproteomics by mass spectrometry is rapidly evolving in many research areas, leading to a demand in reliable data analysis tools.
33363672	6	24	theme	MS/MS	1054:1058	arg1	data					1060:1063	MS/MS data	1054:1063	MS/MS data	1054:1063	Initial glycopeptide identification based on MS/MS data was aided by the Byonic software.
33363672	9	25	theme	glycosylation	1463:1475	arg1	profiles					1477:1484	comparable glycosylation profiles	1452:1484	comparable glycosylation profiles	1452:1484	All quantification packages resulted in comparable glycosylation profiles but featured differences in terms of robustness and data quality control.
33363672	7	26	theme	MS1-based	1110:1118	arg1	relying					1148:1154	Additional MS1-based glycopeptide identification relying	1099:1154	Additional MS1-based glycopeptide identification relying on accurate mass and retention time differences using GlycopeptideGraphMS	1099:1228	Additional MS1-based glycopeptide identification relying on accurate mass and retention time differences using GlycopeptideGraphMS considerably expanded the set of confidently annotated glycopeptides.
33363672	2	27	from	areas	348:352	arg1	use					245:247	The use	241:247	The use of glycopeptide-centered glycoproteomics by mass spectrometry	241:309	The use of glycopeptide-centered glycoproteomics by mass spectrometry is rapidly evolving in many research areas, leading to a demand in reliable data analysis tools.
33363672	2	27	from	areas	348:352	arg1	evolving					322:329	evolving	322:329	evolving	322:329	The use of glycopeptide-centered glycoproteomics by mass spectrometry is rapidly evolving in many research areas, leading to a demand in reliable data analysis tools.
33363672	11	28	theme	semiautomated	1750:1762	arg1	workflow					1764:1771	a semiautomated workflow	1748:1771	a semiautomated workflow for reliable glycoproteomic data analysis by the combination of software packages for MS/MS- and MS1-based glycopeptide identification as well as the integration of analyte quality control and quantification	1748:1979	Finally, this study presents a semiautomated workflow for reliable glycoproteomic data analysis by the combination of software packages for MS/MS- and MS1-based glycopeptide identification as well as the integration of analyte quality control and quantification.
33363672	0	29	theme	reliable	99:106	arg1	quantification					108:121	reliable quantification	99:121	reliable quantification	99:121	Semiautomated glycoproteomics data analysis workflow for maximized glycopeptide identification and reliable quantification.
33363672	1	30	theme	structural	188:197	arg1	diversity					199:207	the high structural diversity	179:207	the high structural diversity of peptide and glycan portions	179:238	Glycoproteomic data are often very complex, reflecting the high structural diversity of peptide and glycan portions.
33363672	5	31	theme	affinity-purified	778:794	arg1	immunoglobulins					796:810	affinity-purified immunoglobulins G and A	778:818	affinity-purified immunoglobulins G and A	778:818	For this purpose, a tryptic digest from affinity-purified immunoglobulins G and A was analyzed on a nano-reversed-phase liquid chromatography-tandem mass spectrometry platform with a high-resolution mass analyzer and higher-energy collisional dissociation fragmentation.
33363672	5	31	theme	affinity-purified	778:794	arg1	A					818:818	A	818:818	A	818:818	For this purpose, a tryptic digest from affinity-purified immunoglobulins G and A was analyzed on a nano-reversed-phase liquid chromatography-tandem mass spectrometry platform with a high-resolution mass analyzer and higher-energy collisional dissociation fragmentation.
33363672	5	31	theme	affinity-purified	778:794	arg1	G					812:812	G	812:812	G	812:812	For this purpose, a tryptic digest from affinity-purified immunoglobulins G and A was analyzed on a nano-reversed-phase liquid chromatography-tandem mass spectrometry platform with a high-resolution mass analyzer and higher-energy collisional dissociation fragmentation.
33363672	10	32	theme	glycopeptides	1704:1716	arg1	oxidation					1577:1585	Partial cysteine oxidation	1560:1585	Partial cysteine oxidation	1560:1585	Partial cysteine oxidation was identified as an unexpectedly abundant peptide modification and impaired the automated processing of several IgA glycopeptides.
33363672	10	32	theme	glycopeptides	1704:1716	arg1	processing					1678:1687	the automated processing	1664:1687	the automated processing of several IgA glycopeptides	1664:1716	Partial cysteine oxidation was identified as an unexpectedly abundant peptide modification and impaired the automated processing of several IgA glycopeptides.
33363672	10	32	theme	glycopeptides	1704:1716	arg1	modification					1638:1649	an unexpectedly abundant peptide modification	1605:1649	an unexpectedly abundant peptide modification	1605:1649	Partial cysteine oxidation was identified as an unexpectedly abundant peptide modification and impaired the automated processing of several IgA glycopeptides.
33363672	9	33	theme	robustness	1523:1532	arg1	terms					1514:1518	terms	1514:1518	terms of robustness and data quality control	1514:1557	All quantification packages resulted in comparable glycosylation profiles but featured differences in terms of robustness and data quality control.
33363672	3	34	theme	recent	411:416	arg1	years					418:422	recent years	411:422	recent years	411:422	In recent years, several bioinformatic tools were developed to facilitate and improve both the identification and quantification of glycopeptides.
33363672	10	35	theme	several	1692:1698	arg1	glycopeptides					1704:1716	several IgA glycopeptides	1692:1716	several IgA glycopeptides	1692:1716	Partial cysteine oxidation was identified as an unexpectedly abundant peptide modification and impaired the automated processing of several IgA glycopeptides.
33363672	0	36	theme	Semiautomated	0:12	arg1	analysis					35:42	Semiautomated glycoproteomics data analysis	0:42	Semiautomated glycoproteomics data analysis	0:42	Semiautomated glycoproteomics data analysis workflow for maximized glycopeptide identification and reliable quantification.
33363672	9	37	theme	data	1538:1541	arg1	control					1551:1557	data quality control	1538:1557	data quality control	1538:1557	All quantification packages resulted in comparable glycosylation profiles but featured differences in terms of robustness and data quality control.
33363672	1	38	theme	Glycoproteomic	124:137	arg1	data					139:142	Glycoproteomic data	124:142	Glycoproteomic data	124:142	Glycoproteomic data are often very complex, reflecting the high structural diversity of peptide and glycan portions.
33363672	4	39	theme	tools	582:586	arg1	selection					563:571	a selection	561:571	a selection of these tools	561:586	Here, a selection of these tools was combined and evaluated with the aim of establishing a robust glycopeptide detection and quantification workflow targeting enriched glycoproteins.
33363672	0	40	theme	data	30:33	arg1	analysis					35:42	Semiautomated glycoproteomics data analysis	0:42	Semiautomated glycoproteomics data analysis	0:42	Semiautomated glycoproteomics data analysis workflow for maximized glycopeptide identification and reliable quantification.
33363672	9	41	theme	control	1551:1557	arg1	terms					1514:1518	terms	1514:1518	terms of robustness and data quality control	1514:1557	All quantification packages resulted in comparable glycosylation profiles but featured differences in terms of robustness and data quality control.
33363672	3	42	theme	bioinformatic	433:445	arg1	tools					447:451	several bioinformatic tools	425:451	several bioinformatic tools	425:451	In recent years, several bioinformatic tools were developed to facilitate and improve both the identification and quantification of glycopeptides.
33363672	7	43	gly	glycopeptides	1285:1297	arg2	glycopeptides					1285:1297	confidently annotated glycopeptides	1263:1297	confidently annotated glycopeptides	1263:1297	Additional MS1-based glycopeptide identification relying on accurate mass and retention time differences using GlycopeptideGraphMS considerably expanded the set of confidently annotated glycopeptides.
33363672	11	44	theme	quality	1946:1952	arg1	control					1954:1960	analyte quality control	1938:1960	analyte quality control	1938:1960	Finally, this study presents a semiautomated workflow for reliable glycoproteomic data analysis by the combination of software packages for MS/MS- and MS1-based glycopeptide identification as well as the integration of analyte quality control and quantification.
33363672	8	45	gly	glycopeptide	1304:1315	arg2	glycopeptide					1304:1315	glycopeptide quantification	1304:1330	glycopeptide quantification	1304:1330	For glycopeptide quantification, the performance of LaCyTools was compared to Skyline, and GlycopeptideGraphMS.
33363672	5	46	theme	liquid	858:863	arg1	spectrometry					892:903	a nano-reversed-phase liquid chromatography-tandem mass spectrometry	836:903	a nano-reversed-phase liquid chromatography-tandem mass spectrometry platform with a high-resolution mass analyzer and higher-energy collisional dissociation fragmentation	836:1006	For this purpose, a tryptic digest from affinity-purified immunoglobulins G and A was analyzed on a nano-reversed-phase liquid chromatography-tandem mass spectrometry platform with a high-resolution mass analyzer and higher-energy collisional dissociation fragmentation.
33363672	11	47	theme	MS1-based	1870:1878	arg1	identification					1893:1906	MS/MS- and MS1-based glycopeptide identification	1859:1906	MS/MS- and MS1-based glycopeptide identification	1859:1906	Finally, this study presents a semiautomated workflow for reliable glycoproteomic data analysis by the combination of software packages for MS/MS- and MS1-based glycopeptide identification as well as the integration of analyte quality control and quantification.
33363672	5	48	theme	nano-reversed-phase	838:856	arg1	spectrometry					892:903	a nano-reversed-phase liquid chromatography-tandem mass spectrometry	836:903	a nano-reversed-phase liquid chromatography-tandem mass spectrometry platform with a high-resolution mass analyzer and higher-energy collisional dissociation fragmentation	836:1006	For this purpose, a tryptic digest from affinity-purified immunoglobulins G and A was analyzed on a nano-reversed-phase liquid chromatography-tandem mass spectrometry platform with a high-resolution mass analyzer and higher-energy collisional dissociation fragmentation.
33363672	0	49	gly	glycopeptide	67:78	arg2	glycopeptide					67:78	maximized glycopeptide identification	57:93	maximized glycopeptide identification	57:93	Semiautomated glycoproteomics data analysis workflow for maximized glycopeptide identification and reliable quantification.
33363672	7	50	theme	glycopeptides	1285:1297	arg1	set					1256:1258	the set	1252:1258	the set of confidently annotated glycopeptides	1252:1297	Additional MS1-based glycopeptide identification relying on accurate mass and retention time differences using GlycopeptideGraphMS considerably expanded the set of confidently annotated glycopeptides.
33363672	7	50	theme	glycopeptides	1285:1297	arg1	glycopeptides					1285:1297	confidently annotated glycopeptides	1263:1297	confidently annotated glycopeptides	1263:1297	Additional MS1-based glycopeptide identification relying on accurate mass and retention time differences using GlycopeptideGraphMS considerably expanded the set of confidently annotated glycopeptides.
33363672	10	51	theme	Partial	1560:1566	arg1	modification					1638:1649	an unexpectedly abundant peptide modification	1605:1649	an unexpectedly abundant peptide modification	1605:1649	Partial cysteine oxidation was identified as an unexpectedly abundant peptide modification and impaired the automated processing of several IgA glycopeptides.
33363672	10	51	theme	Partial	1560:1566	arg1	processing					1678:1687	the automated processing	1664:1687	the automated processing of several IgA glycopeptides	1664:1716	Partial cysteine oxidation was identified as an unexpectedly abundant peptide modification and impaired the automated processing of several IgA glycopeptides.
33363672	10	51	theme	Partial	1560:1566	arg1	oxidation					1577:1585	Partial cysteine oxidation	1560:1585	Partial cysteine oxidation	1560:1585	Partial cysteine oxidation was identified as an unexpectedly abundant peptide modification and impaired the automated processing of several IgA glycopeptides.
33363672	2	52	theme	data	387:390	arg1	tools					401:405	reliable data analysis tools	378:405	reliable data analysis tools	378:405	The use of glycopeptide-centered glycoproteomics by mass spectrometry is rapidly evolving in many research areas, leading to a demand in reliable data analysis tools.
33363672	11	53	theme	glycoproteomic	1786:1799	arg1	analysis					1806:1813	reliable glycoproteomic data analysis	1777:1813	reliable glycoproteomic data analysis by the combination of software packages	1777:1853	Finally, this study presents a semiautomated workflow for reliable glycoproteomic data analysis by the combination of software packages for MS/MS- and MS1-based glycopeptide identification as well as the integration of analyte quality control and quantification.
33363672	2	54	theme	many	334:337	arg1	areas					348:352	many research areas	334:352	many research areas	334:352	The use of glycopeptide-centered glycoproteomics by mass spectrometry is rapidly evolving in many research areas, leading to a demand in reliable data analysis tools.
33363672	6	55	theme	Initial	1009:1015	arg1	identification					1030:1043	Initial glycopeptide identification	1009:1043	Initial glycopeptide identification based on MS/MS data	1009:1063	Initial glycopeptide identification based on MS/MS data was aided by the Byonic software.
33363672	11	56	theme	packages	1846:1853	arg1	combination					1822:1832	the combination	1818:1832	the combination of software packages	1818:1853	Finally, this study presents a semiautomated workflow for reliable glycoproteomic data analysis by the combination of software packages for MS/MS- and MS1-based glycopeptide identification as well as the integration of analyte quality control and quantification.
33363672	4	57	gly	glycopeptide	653:664	arg2	glycopeptide					653:664	a robust glycopeptide detection	644:674	a robust glycopeptide detection	644:674	Here, a selection of these tools was combined and evaluated with the aim of establishing a robust glycopeptide detection and quantification workflow targeting enriched glycoproteins.
33363672	1	58	theme	peptide	212:218	arg1	diversity					199:207	the high structural diversity	179:207	the high structural diversity of peptide and glycan portions	179:238	Glycoproteomic data are often very complex, reflecting the high structural diversity of peptide and glycan portions.
33363672	0	59	theme	maximized	57:65	arg1	identification					80:93	maximized glycopeptide identification	57:93	maximized glycopeptide identification	57:93	Semiautomated glycoproteomics data analysis workflow for maximized glycopeptide identification and reliable quantification.
33363672	4	60	theme	workflow	695:702	arg1	glycoproteins					723:735	workflow targeting enriched glycoproteins	695:735	workflow targeting enriched glycoproteins	695:735	Here, a selection of these tools was combined and evaluated with the aim of establishing a robust glycopeptide detection and quantification workflow targeting enriched glycoproteins.
33363672	5	61	theme	higher-energy	955:967	arg1	fragmentation					994:1006	higher-energy collisional dissociation fragmentation	955:1006	higher-energy collisional dissociation fragmentation	955:1006	For this purpose, a tryptic digest from affinity-purified immunoglobulins G and A was analyzed on a nano-reversed-phase liquid chromatography-tandem mass spectrometry platform with a high-resolution mass analyzer and higher-energy collisional dissociation fragmentation.
33363672	5	62	with	platform	905:912	arg1	fragmentation					994:1006	higher-energy collisional dissociation fragmentation	955:1006	higher-energy collisional dissociation fragmentation	955:1006	For this purpose, a tryptic digest from affinity-purified immunoglobulins G and A was analyzed on a nano-reversed-phase liquid chromatography-tandem mass spectrometry platform with a high-resolution mass analyzer and higher-energy collisional dissociation fragmentation.
33363672	5	62	with	platform	905:912	arg1	analyzer					942:949	a high-resolution mass analyzer	919:949	a high-resolution mass analyzer	919:949	For this purpose, a tryptic digest from affinity-purified immunoglobulins G and A was analyzed on a nano-reversed-phase liquid chromatography-tandem mass spectrometry platform with a high-resolution mass analyzer and higher-energy collisional dissociation fragmentation.
33363672	4	63	theme	enriched	714:721	arg1	glycoproteins					723:735	workflow targeting enriched glycoproteins	695:735	workflow targeting enriched glycoproteins	695:735	Here, a selection of these tools was combined and evaluated with the aim of establishing a robust glycopeptide detection and quantification workflow targeting enriched glycoproteins.
33363672	11	64	theme	data	1801:1804	arg1	analysis					1806:1813	reliable glycoproteomic data analysis	1777:1813	reliable glycoproteomic data analysis by the combination of software packages	1777:1853	Finally, this study presents a semiautomated workflow for reliable glycoproteomic data analysis by the combination of software packages for MS/MS- and MS1-based glycopeptide identification as well as the integration of analyte quality control and quantification.
33363672	10	65	theme	abundant	1621:1628	arg1	oxidation					1577:1585	Partial cysteine oxidation	1560:1585	Partial cysteine oxidation	1560:1585	Partial cysteine oxidation was identified as an unexpectedly abundant peptide modification and impaired the automated processing of several IgA glycopeptides.
33363672	10	65	theme	abundant	1621:1628	arg1	processing					1678:1687	the automated processing	1664:1687	the automated processing of several IgA glycopeptides	1664:1716	Partial cysteine oxidation was identified as an unexpectedly abundant peptide modification and impaired the automated processing of several IgA glycopeptides.
33363672	10	65	theme	abundant	1621:1628	arg1	modification					1638:1649	an unexpectedly abundant peptide modification	1605:1649	an unexpectedly abundant peptide modification	1605:1649	Partial cysteine oxidation was identified as an unexpectedly abundant peptide modification and impaired the automated processing of several IgA glycopeptides.
33363672	1	66	theme	glycan	224:229	arg1	portions					231:238	glycan portions	224:238	glycan portions	224:238	Glycoproteomic data are often very complex, reflecting the high structural diversity of peptide and glycan portions.
33363672	4	67	theme	glycopeptide	653:664	arg1	detection					666:674	a robust glycopeptide detection	644:674	a robust glycopeptide detection	644:674	Here, a selection of these tools was combined and evaluated with the aim of establishing a robust glycopeptide detection and quantification workflow targeting enriched glycoproteins.
33363672	5	68	theme	tryptic	758:764	arg1	digest					766:771	a tryptic digest	756:771	a tryptic digest from affinity-purified immunoglobulins G and A	756:818	For this purpose, a tryptic digest from affinity-purified immunoglobulins G and A was analyzed on a nano-reversed-phase liquid chromatography-tandem mass spectrometry platform with a high-resolution mass analyzer and higher-energy collisional dissociation fragmentation.
33363672	9	69	theme	quantification	1416:1429	arg1	packages					1431:1438	All quantification packages	1412:1438	All quantification packages	1412:1438	All quantification packages resulted in comparable glycosylation profiles but featured differences in terms of robustness and data quality control.
33363672	2	70	theme	glycoproteomics	274:288	arg1	use					245:247	The use	241:247	The use of glycopeptide-centered glycoproteomics by mass spectrometry	241:309	The use of glycopeptide-centered glycoproteomics by mass spectrometry is rapidly evolving in many research areas, leading to a demand in reliable data analysis tools.
33363672	2	70	theme	glycoproteomics	274:288	arg1	evolving					322:329	evolving	322:329	evolving	322:329	The use of glycopeptide-centered glycoproteomics by mass spectrometry is rapidly evolving in many research areas, leading to a demand in reliable data analysis tools.
33363672	5	71	theme	high-resolution	921:935	arg1	analyzer					942:949	a high-resolution mass analyzer	919:949	a high-resolution mass analyzer	919:949	For this purpose, a tryptic digest from affinity-purified immunoglobulins G and A was analyzed on a nano-reversed-phase liquid chromatography-tandem mass spectrometry platform with a high-resolution mass analyzer and higher-energy collisional dissociation fragmentation.
33363672	11	72	gly	glycopeptide	1880:1891	arg2	glycopeptide					1880:1891	MS/MS- and MS1-based glycopeptide identification	1859:1906	MS/MS- and MS1-based glycopeptide identification	1859:1906	Finally, this study presents a semiautomated workflow for reliable glycoproteomic data analysis by the combination of software packages for MS/MS- and MS1-based glycopeptide identification as well as the integration of analyte quality control and quantification.
33363672	7	73	theme	retention	1177:1185	arg1	time					1187:1190	retention time	1177:1190	retention time	1177:1190	Additional MS1-based glycopeptide identification relying on accurate mass and retention time differences using GlycopeptideGraphMS considerably expanded the set of confidently annotated glycopeptides.
33363672	7	74	theme	accurate	1159:1166	arg1	mass					1168:1171	accurate mass	1159:1171	accurate mass	1159:1171	Additional MS1-based glycopeptide identification relying on accurate mass and retention time differences using GlycopeptideGraphMS considerably expanded the set of confidently annotated glycopeptides.
33363672	7	75	theme	mass	1168:1171	arg1	differences					1192:1202	accurate mass and retention time differences	1159:1202	accurate mass and retention time differences using GlycopeptideGraphMS	1159:1228	Additional MS1-based glycopeptide identification relying on accurate mass and retention time differences using GlycopeptideGraphMS considerably expanded the set of confidently annotated glycopeptides.
33363672	9	76	theme	comparable	1452:1461	arg1	profiles					1477:1484	comparable glycosylation profiles	1452:1484	comparable glycosylation profiles	1452:1484	All quantification packages resulted in comparable glycosylation profiles but featured differences in terms of robustness and data quality control.
33363672	2	77	theme	mass	293:296	arg1	spectrometry					298:309	mass spectrometry	293:309	mass spectrometry	293:309	The use of glycopeptide-centered glycoproteomics by mass spectrometry is rapidly evolving in many research areas, leading to a demand in reliable data analysis tools.
33363672	7	78	theme	glycopeptide	1120:1131	arg1	relying					1148:1154	Additional MS1-based glycopeptide identification relying	1099:1154	Additional MS1-based glycopeptide identification relying on accurate mass and retention time differences using GlycopeptideGraphMS	1099:1228	Additional MS1-based glycopeptide identification relying on accurate mass and retention time differences using GlycopeptideGraphMS considerably expanded the set of confidently annotated glycopeptides.
33363672	5	79	theme	dissociation	981:992	arg1	fragmentation					994:1006	higher-energy collisional dissociation fragmentation	955:1006	higher-energy collisional dissociation fragmentation	955:1006	For this purpose, a tryptic digest from affinity-purified immunoglobulins G and A was analyzed on a nano-reversed-phase liquid chromatography-tandem mass spectrometry platform with a high-resolution mass analyzer and higher-energy collisional dissociation fragmentation.
33363672	9	80	theme	featured	1490:1497	arg1	differences					1499:1509	featured differences	1490:1509	featured differences in terms of robustness and data quality control	1490:1557	All quantification packages resulted in comparable glycosylation profiles but featured differences in terms of robustness and data quality control.
33363672	7	81	theme	Additional	1099:1108	arg1	relying					1148:1154	Additional MS1-based glycopeptide identification relying	1099:1154	Additional MS1-based glycopeptide identification relying on accurate mass and retention time differences using GlycopeptideGraphMS	1099:1228	Additional MS1-based glycopeptide identification relying on accurate mass and retention time differences using GlycopeptideGraphMS considerably expanded the set of confidently annotated glycopeptides.
33363672	1	82	theme	high	183:186	arg1	diversity					199:207	the high structural diversity	179:207	the high structural diversity of peptide and glycan portions	179:238	Glycoproteomic data are often very complex, reflecting the high structural diversity of peptide and glycan portions.
33363672	10	83	theme	automated	1668:1676	arg1	oxidation					1577:1585	Partial cysteine oxidation	1560:1585	Partial cysteine oxidation	1560:1585	Partial cysteine oxidation was identified as an unexpectedly abundant peptide modification and impaired the automated processing of several IgA glycopeptides.
33363672	10	83	theme	automated	1668:1676	arg1	processing					1678:1687	the automated processing	1664:1687	the automated processing of several IgA glycopeptides	1664:1716	Partial cysteine oxidation was identified as an unexpectedly abundant peptide modification and impaired the automated processing of several IgA glycopeptides.
33363672	10	83	theme	automated	1668:1676	arg1	modification					1638:1649	an unexpectedly abundant peptide modification	1605:1649	an unexpectedly abundant peptide modification	1605:1649	Partial cysteine oxidation was identified as an unexpectedly abundant peptide modification and impaired the automated processing of several IgA glycopeptides.
33363672	6	84	gly	glycopeptide	1017:1028	arg2	glycopeptide					1017:1028	Initial glycopeptide identification	1009:1043	Initial glycopeptide identification based on MS/MS data	1009:1063	Initial glycopeptide identification based on MS/MS data was aided by the Byonic software.
33363672	3	85	dep	identification	503:516	arg1	the					499:501	the	499:501	the	499:501	In recent years, several bioinformatic tools were developed to facilitate and improve both the identification and quantification of glycopeptides.
33363672	7	86	from	relying	1148:1154	arg1	differences					1192:1202	accurate mass and retention time differences	1159:1202	accurate mass and retention time differences using GlycopeptideGraphMS	1159:1228	Additional MS1-based glycopeptide identification relying on accurate mass and retention time differences using GlycopeptideGraphMS considerably expanded the set of confidently annotated glycopeptides.
33363672	10	87	theme	IgA	1700:1702	arg1	glycopeptides					1704:1716	several IgA glycopeptides	1692:1716	several IgA glycopeptides	1692:1716	Partial cysteine oxidation was identified as an unexpectedly abundant peptide modification and impaired the automated processing of several IgA glycopeptides.
33363672	3	88	gly	glycopeptides	540:552	arg2	glycopeptides					540:552	glycopeptides	540:552	glycopeptides	540:552	In recent years, several bioinformatic tools were developed to facilitate and improve both the identification and quantification of glycopeptides.
33363672	0	89	theme	glycoproteomics	14:28	arg1	analysis					35:42	Semiautomated glycoproteomics data analysis	0:42	Semiautomated glycoproteomics data analysis	0:42	Semiautomated glycoproteomics data analysis workflow for maximized glycopeptide identification and reliable quantification.
33363672	7	90	gly	glycopeptide	1120:1131	arg2	glycopeptide					1120:1131	Additional MS1-based glycopeptide identification relying	1099:1154	Additional MS1-based glycopeptide identification relying on accurate mass and retention time differences using GlycopeptideGraphMS	1099:1228	Additional MS1-based glycopeptide identification relying on accurate mass and retention time differences using GlycopeptideGraphMS considerably expanded the set of confidently annotated glycopeptides.
33363672	5	91	theme	spectrometry	892:903	arg1	platform					905:912	a nano-reversed-phase liquid chromatography-tandem mass spectrometry platform	836:912	a nano-reversed-phase liquid chromatography-tandem mass spectrometry platform with a high-resolution mass analyzer and higher-energy collisional dissociation fragmentation	836:1006	For this purpose, a tryptic digest from affinity-purified immunoglobulins G and A was analyzed on a nano-reversed-phase liquid chromatography-tandem mass spectrometry platform with a high-resolution mass analyzer and higher-energy collisional dissociation fragmentation.
33363672	9	92	theme	quality	1543:1549	arg1	control					1551:1557	data quality control	1538:1557	data quality control	1538:1557	All quantification packages resulted in comparable glycosylation profiles but featured differences in terms of robustness and data quality control.
33363672	11	93	theme	control	1954:1960	arg1	integration					1923:1933	the integration	1919:1933	the integration of analyte quality control and quantification	1919:1979	Finally, this study presents a semiautomated workflow for reliable glycoproteomic data analysis by the combination of software packages for MS/MS- and MS1-based glycopeptide identification as well as the integration of analyte quality control and quantification.
33363672	11	93	theme	control	1954:1960	arg1	identification					1893:1906	MS/MS- and MS1-based glycopeptide identification	1859:1906	MS/MS- and MS1-based glycopeptide identification	1859:1906	Finally, this study presents a semiautomated workflow for reliable glycoproteomic data analysis by the combination of software packages for MS/MS- and MS1-based glycopeptide identification as well as the integration of analyte quality control and quantification.
33363672	11	94	theme	analyte	1938:1944	arg1	control					1954:1960	analyte quality control	1938:1960	analyte quality control	1938:1960	Finally, this study presents a semiautomated workflow for reliable glycoproteomic data analysis by the combination of software packages for MS/MS- and MS1-based glycopeptide identification as well as the integration of analyte quality control and quantification.
33363672	11	95	theme	software	1837:1844	arg1	packages					1846:1853	software packages	1837:1853	software packages	1837:1853	Finally, this study presents a semiautomated workflow for reliable glycoproteomic data analysis by the combination of software packages for MS/MS- and MS1-based glycopeptide identification as well as the integration of analyte quality control and quantification.
33363672	0	96	theme	glycopeptide	67:78	arg1	identification					80:93	maximized glycopeptide identification	57:93	maximized glycopeptide identification	57:93	Semiautomated glycoproteomics data analysis workflow for maximized glycopeptide identification and reliable quantification.
33363672	4	97	dep	detection	666:674	arg1	glycoproteins					723:735	workflow targeting enriched glycoproteins	695:735	workflow targeting enriched glycoproteins	695:735	Here, a selection of these tools was combined and evaluated with the aim of establishing a robust glycopeptide detection and quantification workflow targeting enriched glycoproteins.
33363672	3	98	theme	several	425:431	arg1	tools					447:451	several bioinformatic tools	425:451	several bioinformatic tools	425:451	In recent years, several bioinformatic tools were developed to facilitate and improve both the identification and quantification of glycopeptides.
33363672	4	99	gly	glycoproteins	723:735	arg1	glycoproteins					723:735	workflow targeting enriched glycoproteins	695:735	workflow targeting enriched glycoproteins	695:735	Here, a selection of these tools was combined and evaluated with the aim of establishing a robust glycopeptide detection and quantification workflow targeting enriched glycoproteins.
33363672	10	100	gly	glycopeptides	1704:1716	arg2	glycopeptides					1704:1716	several IgA glycopeptides	1692:1716	several IgA glycopeptides	1692:1716	Partial cysteine oxidation was identified as an unexpectedly abundant peptide modification and impaired the automated processing of several IgA glycopeptides.
33363672	11	101	theme	quantification	1966:1979	arg1	integration					1923:1933	the integration	1919:1933	the integration of analyte quality control and quantification	1919:1979	Finally, this study presents a semiautomated workflow for reliable glycoproteomic data analysis by the combination of software packages for MS/MS- and MS1-based glycopeptide identification as well as the integration of analyte quality control and quantification.
33363672	11	101	theme	quantification	1966:1979	arg1	identification					1893:1906	MS/MS- and MS1-based glycopeptide identification	1859:1906	MS/MS- and MS1-based glycopeptide identification	1859:1906	Finally, this study presents a semiautomated workflow for reliable glycoproteomic data analysis by the combination of software packages for MS/MS- and MS1-based glycopeptide identification as well as the integration of analyte quality control and quantification.
33363672	2	102	theme	analysis	392:399	arg1	tools					401:405	reliable data analysis tools	378:405	reliable data analysis tools	378:405	The use of glycopeptide-centered glycoproteomics by mass spectrometry is rapidly evolving in many research areas, leading to a demand in reliable data analysis tools.
33363672	8	103	theme	glycopeptide	1304:1315	arg1	quantification					1317:1330	glycopeptide quantification	1304:1330	glycopeptide quantification	1304:1330	For glycopeptide quantification, the performance of LaCyTools was compared to Skyline, and GlycopeptideGraphMS.
33363672	4	104	theme	robust	646:651	arg1	detection					666:674	a robust glycopeptide detection	644:674	a robust glycopeptide detection	644:674	Here, a selection of these tools was combined and evaluated with the aim of establishing a robust glycopeptide detection and quantification workflow targeting enriched glycoproteins.
33363672	10	105	theme	cysteine	1568:1575	arg1	modification					1638:1649	an unexpectedly abundant peptide modification	1605:1649	an unexpectedly abundant peptide modification	1605:1649	Partial cysteine oxidation was identified as an unexpectedly abundant peptide modification and impaired the automated processing of several IgA glycopeptides.
33363672	10	105	theme	cysteine	1568:1575	arg1	processing					1678:1687	the automated processing	1664:1687	the automated processing of several IgA glycopeptides	1664:1716	Partial cysteine oxidation was identified as an unexpectedly abundant peptide modification and impaired the automated processing of several IgA glycopeptides.
33363672	10	105	theme	cysteine	1568:1575	arg1	oxidation					1577:1585	Partial cysteine oxidation	1560:1585	Partial cysteine oxidation	1560:1585	Partial cysteine oxidation was identified as an unexpectedly abundant peptide modification and impaired the automated processing of several IgA glycopeptides.
33363672	5	106	theme	chromatography-tandem	865:885	arg1	spectrometry					892:903	a nano-reversed-phase liquid chromatography-tandem mass spectrometry	836:903	a nano-reversed-phase liquid chromatography-tandem mass spectrometry platform with a high-resolution mass analyzer and higher-energy collisional dissociation fragmentation	836:1006	For this purpose, a tryptic digest from affinity-purified immunoglobulins G and A was analyzed on a nano-reversed-phase liquid chromatography-tandem mass spectrometry platform with a high-resolution mass analyzer and higher-energy collisional dissociation fragmentation.
32908918	11	0	theme	KEGG	1664:1667	arg1	pathways					1669:1676	13 KEGG pathways	1661:1676	13 KEGG pathways	1661:1676	Among these KEGG pathways, 13 KEGG pathways were involved in host metabolic function related to amino acid metabolism, carbohydrate metabolism, metabolism of cofactors and vitamins, and metabolism of terpenoids and polyketides.
32908918	8	1	theme	Prevotellaceae	1150:1163	arg1	family					1165:1170	the Prevotellaceae family	1146:1170	the Prevotellaceae family	1146:1170	Meanwhile, the abundance of the Prevotellaceae family and Parasutterela genus and the Alcaligencaeae family and Burkholderiales order and its attachment to the Betaproteobacteria class were overrepresented in the 3DG group.
32908918	11	2	theme	metabolic	1700:1708	arg1	function					1710:1717	host metabolic function	1695:1717	host metabolic function related to amino acid metabolism, carbohydrate metabolism, metabolism of cofactors and vitamins, and metabolism of terpenoids and polyketides	1695:1859	Among these KEGG pathways, 13 KEGG pathways were involved in host metabolic function related to amino acid metabolism, carbohydrate metabolism, metabolism of cofactors and vitamins, and metabolism of terpenoids and polyketides.
32908918	8	3	attach	attachment	1260:1269	arg2	family					1219:1224	the Alcaligencaeae family	1200:1224	the Alcaligencaeae family	1200:1224	Meanwhile, the abundance of the Prevotellaceae family and Parasutterela genus and the Alcaligencaeae family and Burkholderiales order and its attachment to the Betaproteobacteria class were overrepresented in the 3DG group.
32908918	8	3	attach	attachment	1260:1269	arg2	family					1165:1170	the Prevotellaceae family	1146:1170	the Prevotellaceae family	1146:1170	Meanwhile, the abundance of the Prevotellaceae family and Parasutterela genus and the Alcaligencaeae family and Burkholderiales order and its attachment to the Betaproteobacteria class were overrepresented in the 3DG group.
32908918	8	3	attach	attachment	1260:1269	arg1	class					1297:1301	the Betaproteobacteria class	1274:1301	the Betaproteobacteria class	1274:1301	Meanwhile, the abundance of the Prevotellaceae family and Parasutterela genus and the Alcaligencaeae family and Burkholderiales order and its attachment to the Betaproteobacteria class were overrepresented in the 3DG group.
32908918	12	4	theme	oral	2050:2053	arg1	tolerance					2063:2071	oral glucose tolerance	2050:2071	oral glucose tolerance	2050:2071	Moreover, the increased LPS levels and the decreased GLP-2 concentration in plasma and intestinal tissues were observed in 3DG-treated rats, together with the impaired fasting glucose and oral glucose tolerance.
32908918	4	5	theme	gene	666:669	arg1	sequence					671:678	the 16S rRNA gene sequence	653:678	the 16S rRNA gene sequence	653:678	Microbial profiling in faeces samples was determined through the 16S rRNA gene sequence.
32908918	10	6	theme	two-week	1589:1596	arg1	administration					1611:1624	two-week intragastric administration	1589:1624	two-week intragastric administration of 3DG	1589:1631	In addition, 45 KEGG pathways were altered after two-week intragastric administration of 3DG.
32908918	3	7	theme	50 mg/kg	533:540	arg1	3DG					542:544	50 mg/kg 3DG	533:544	50 mg/kg 3DG	533:544	Rats were given 50 mg/kg 3DG by intragastric administration for two weeks.
32908918	1	8	theme	Maillard	182:189	arg1	reactions					191:199	Maillard reactions	182:199	Maillard reactions	182:199	Our previous research suggests that 3-deoxyglucosone (3DG), formed in the caramelization course and Maillard reactions in food, is an independent factor for the development of prediabetes.
32908918	5	9	theme	glucagon-like	685:697	arg1	peptide					699:705	glucagon-like peptide 2	685:707	glucagon-like peptide 2 (GLP-2)	685:715	The glucagon-like peptide 2 (GLP-2) and lipopolysaccharide (LPS) levels in plasma and intestinal tissues were measured by ELISA and Limulus test, respectively.
32908918	5	9	theme	glucagon-like	685:697	arg1	GLP-2					710:714	GLP-2	710:714	GLP-2	710:714	The glucagon-like peptide 2 (GLP-2) and lipopolysaccharide (LPS) levels in plasma and intestinal tissues were measured by ELISA and Limulus test, respectively.
32908918	11	10	theme	terpenoids	1834:1843	arg1	metabolism					1820:1829	metabolism	1820:1829	metabolism of terpenoids and polyketides	1820:1859	Among these KEGG pathways, 13 KEGG pathways were involved in host metabolic function related to amino acid metabolism, carbohydrate metabolism, metabolism of cofactors and vitamins, and metabolism of terpenoids and polyketides.
32908918	11	10	theme	terpenoids	1834:1843	arg1	metabolism					1778:1787	metabolism	1778:1787	metabolism of cofactors and vitamins	1778:1813	Among these KEGG pathways, 13 KEGG pathways were involved in host metabolic function related to amino acid metabolism, carbohydrate metabolism, metabolism of cofactors and vitamins, and metabolism of terpenoids and polyketides.
32908918	11	10	theme	terpenoids	1834:1843	arg1	metabolism					1741:1750	amino acid metabolism	1730:1750	amino acid metabolism	1730:1750	Among these KEGG pathways, 13 KEGG pathways were involved in host metabolic function related to amino acid metabolism, carbohydrate metabolism, metabolism of cofactors and vitamins, and metabolism of terpenoids and polyketides.
32908918	11	10	theme	terpenoids	1834:1843	arg1	metabolism					1766:1775	carbohydrate metabolism	1753:1775	carbohydrate metabolism	1753:1775	Among these KEGG pathways, 13 KEGG pathways were involved in host metabolic function related to amino acid metabolism, carbohydrate metabolism, metabolism of cofactors and vitamins, and metabolism of terpenoids and polyketides.
32908918	12	11	theme	impaired	2021:2028	arg1	glucose					2038:2044	the impaired fasting glucose	2017:2044	the impaired fasting glucose	2017:2044	Moreover, the increased LPS levels and the decreased GLP-2 concentration in plasma and intestinal tissues were observed in 3DG-treated rats, together with the impaired fasting glucose and oral glucose tolerance.
32908918	9	12	from	group	1533:1537	arg1	abundant					1509:1516	abundant	1509:1516	abundant	1509:1516	The bacteria of Candidatus Soleaferrea genus, Gelria genus, and Thermoanaerobacteraceae family and its attachment to Thermoanaerobacterales order were apparently more abundant in the control group.
32908918	9	12	from	group	1533:1537	arg1	bacteria					1346:1353	The bacteria	1342:1353	The bacteria of Candidatus Soleaferrea genus, Gelria genus, and Thermoanaerobacteraceae family and its attachment to Thermoanaerobacterales order	1342:1486	The bacteria of Candidatus Soleaferrea genus, Gelria genus, and Thermoanaerobacteraceae family and its attachment to Thermoanaerobacterales order were apparently more abundant in the control group.
32908918	10	13	theme	3DG	1629:1631	arg1	administration					1611:1624	two-week intragastric administration	1589:1624	two-week intragastric administration of 3DG	1589:1631	In addition, 45 KEGG pathways were altered after two-week intragastric administration of 3DG.
32908918	13	14	theme	3DG-treated	2164:2174	arg1	rats					2176:2179	3DG-treated rats	2164:2179	3DG-treated rats	2164:2179	The alterations in composition and function of the intestinal microbiota were observed in 3DG-treated rats, which provides a possible mechanism linking exogenous 3DG intake to the development of prediabetes.
32908918	2	15	theme	type	302:305	arg1	T2D					319:321	T2D	319:321	T2D	319:321	Since the relationship between type 2 diabetes (T2D) and intestinal microbiota is moving from correlation to causality, we investigated the alterations in the composition and function of the intestinal microbiota in 3DG-induced prediabetic rats.
32908918	2	15	theme	type	302:305	arg1	diabetes					309:316	type 2 diabetes	302:316	type 2 diabetes (T2D)	302:322	Since the relationship between type 2 diabetes (T2D) and intestinal microbiota is moving from correlation to causality, we investigated the alterations in the composition and function of the intestinal microbiota in 3DG-induced prediabetic rats.
32908918	8	16	theme	family	1219:1224	arg1	abundance					1133:1141	the abundance	1129:1141	the abundance of the Prevotellaceae family and Parasutterela genus and the Alcaligencaeae family and Burkholderiales order	1129:1250	Meanwhile, the abundance of the Prevotellaceae family and Parasutterela genus and the Alcaligencaeae family and Burkholderiales order and its attachment to the Betaproteobacteria class were overrepresented in the 3DG group.
32908918	8	16	theme	family	1219:1224	arg1	attachment					1260:1269	its attachment	1256:1269	its attachment to the Betaproteobacteria class	1256:1301	Meanwhile, the abundance of the Prevotellaceae family and Parasutterela genus and the Alcaligencaeae family and Burkholderiales order and its attachment to the Betaproteobacteria class were overrepresented in the 3DG group.
32908918	8	16	theme	family	1219:1224	arg1	Meanwhile					1118:1126	Meanwhile	1118:1126	Meanwhile	1118:1126	Meanwhile, the abundance of the Prevotellaceae family and Parasutterela genus and the Alcaligencaeae family and Burkholderiales order and its attachment to the Betaproteobacteria class were overrepresented in the 3DG group.
32908918	6	17	theme	microbiota	953:962	arg1	composition					927:937	the composition	923:937	the composition of intestinal microbiota	923:962	3DG treatment did not significantly change the richness and evenness but affected the composition of intestinal microbiota.
32908918	4	18	theme	16S	657:659	arg1	sequence					671:678	the 16S rRNA gene sequence	653:678	the 16S rRNA gene sequence	653:678	Microbial profiling in faeces samples was determined through the 16S rRNA gene sequence.
32908918	8	19	theme	Burkholderiales	1230:1244	arg1	order					1246:1250	Burkholderiales order	1230:1250	Burkholderiales order	1230:1250	Meanwhile, the abundance of the Prevotellaceae family and Parasutterela genus and the Alcaligencaeae family and Burkholderiales order and its attachment to the Betaproteobacteria class were overrepresented in the 3DG group.
32908918	11	20	theme	polyketides	1849:1859	arg1	metabolism					1820:1829	metabolism	1820:1829	metabolism of terpenoids and polyketides	1820:1859	Among these KEGG pathways, 13 KEGG pathways were involved in host metabolic function related to amino acid metabolism, carbohydrate metabolism, metabolism of cofactors and vitamins, and metabolism of terpenoids and polyketides.
32908918	11	20	theme	polyketides	1849:1859	arg1	metabolism					1778:1787	metabolism	1778:1787	metabolism of cofactors and vitamins	1778:1813	Among these KEGG pathways, 13 KEGG pathways were involved in host metabolic function related to amino acid metabolism, carbohydrate metabolism, metabolism of cofactors and vitamins, and metabolism of terpenoids and polyketides.
32908918	11	20	theme	polyketides	1849:1859	arg1	metabolism					1741:1750	amino acid metabolism	1730:1750	amino acid metabolism	1730:1750	Among these KEGG pathways, 13 KEGG pathways were involved in host metabolic function related to amino acid metabolism, carbohydrate metabolism, metabolism of cofactors and vitamins, and metabolism of terpenoids and polyketides.
32908918	11	20	theme	polyketides	1849:1859	arg1	metabolism					1766:1775	carbohydrate metabolism	1753:1775	carbohydrate metabolism	1753:1775	Among these KEGG pathways, 13 KEGG pathways were involved in host metabolic function related to amino acid metabolism, carbohydrate metabolism, metabolism of cofactors and vitamins, and metabolism of terpenoids and polyketides.
32908918	7	21	theme	Proteobacteria	1048:1061	arg1	abundance					1014:1022	the abundance	1010:1022	the abundance of nondominant bacteria Proteobacteria	1010:1061	At the phylum level, 3DG treatment increased the abundance of nondominant bacteria Proteobacteria but did not cause the change of the dominant bacteria.
32908918	13	22	theme	microbiota	2136:2145	arg1	composition					2093:2103	composition	2093:2103	composition	2093:2103	The alterations in composition and function of the intestinal microbiota were observed in 3DG-treated rats, which provides a possible mechanism linking exogenous 3DG intake to the development of prediabetes.
32908918	13	22	theme	microbiota	2136:2145	arg1	function					2109:2116	function	2109:2116	function	2109:2116	The alterations in composition and function of the intestinal microbiota were observed in 3DG-treated rats, which provides a possible mechanism linking exogenous 3DG intake to the development of prediabetes.
32908918	7	23	theme	nondominant	1027:1037	arg1	Proteobacteria					1048:1061	nondominant bacteria Proteobacteria	1027:1061	nondominant bacteria Proteobacteria	1027:1061	At the phylum level, 3DG treatment increased the abundance of nondominant bacteria Proteobacteria but did not cause the change of the dominant bacteria.
32908918	5	24	from	levels	746:751	arg1	plasma					756:761	plasma	756:761	plasma	756:761	The glucagon-like peptide 2 (GLP-2) and lipopolysaccharide (LPS) levels in plasma and intestinal tissues were measured by ELISA and Limulus test, respectively.
32908918	5	24	from	levels	746:751	arg1	tissues					778:784	intestinal tissues	767:784	intestinal tissues	767:784	The glucagon-like peptide 2 (GLP-2) and lipopolysaccharide (LPS) levels in plasma and intestinal tissues were measured by ELISA and Limulus test, respectively.
32908918	1	25	from	reactions	191:199	arg1	food					204:207	food	204:207	food	204:207	Our previous research suggests that 3-deoxyglucosone (3DG), formed in the caramelization course and Maillard reactions in food, is an independent factor for the development of prediabetes.
32908918	2	26	theme	prediabetic	499:509	arg1	rats					511:514	3DG-induced prediabetic rats	487:514	3DG-induced prediabetic rats	487:514	Since the relationship between type 2 diabetes (T2D) and intestinal microbiota is moving from correlation to causality, we investigated the alterations in the composition and function of the intestinal microbiota in 3DG-induced prediabetic rats.
32908918	1	27	theme	prediabetes	258:268	arg1	development					243:253	the development	239:253	the development of prediabetes	239:268	Our previous research suggests that 3-deoxyglucosone (3DG), formed in the caramelization course and Maillard reactions in food, is an independent factor for the development of prediabetes.
32908918	5	28	theme	Limulus	813:819	arg1	test					821:824	Limulus test	813:824	Limulus test	813:824	The glucagon-like peptide 2 (GLP-2) and lipopolysaccharide (LPS) levels in plasma and intestinal tissues were measured by ELISA and Limulus test, respectively.
32908918	7	29	theme	phylum	972:977	arg1	level					979:983	the phylum level	968:983	the phylum level	968:983	At the phylum level, 3DG treatment increased the abundance of nondominant bacteria Proteobacteria but did not cause the change of the dominant bacteria.
32908918	12	30	theme	intestinal	1949:1958	arg1	tissues					1960:1966	intestinal tissues	1949:1966	intestinal tissues	1949:1966	Moreover, the increased LPS levels and the decreased GLP-2 concentration in plasma and intestinal tissues were observed in 3DG-treated rats, together with the impaired fasting glucose and oral glucose tolerance.
32908918	7	31	theme	bacteria	1108:1115	arg1	change					1085:1090	the change	1081:1090	the change of the dominant bacteria	1081:1115	At the phylum level, 3DG treatment increased the abundance of nondominant bacteria Proteobacteria but did not cause the change of the dominant bacteria.
32908918	2	32	from	composition	430:440	arg1	rats					511:514	3DG-induced prediabetic rats	487:514	3DG-induced prediabetic rats	487:514	Since the relationship between type 2 diabetes (T2D) and intestinal microbiota is moving from correlation to causality, we investigated the alterations in the composition and function of the intestinal microbiota in 3DG-induced prediabetic rats.
32908918	12	33	from	concentration	1921:1933	arg1	plasma					1938:1943	plasma	1938:1943	plasma	1938:1943	Moreover, the increased LPS levels and the decreased GLP-2 concentration in plasma and intestinal tissues were observed in 3DG-treated rats, together with the impaired fasting glucose and oral glucose tolerance.
32908918	12	33	from	concentration	1921:1933	arg1	tissues					1960:1966	intestinal tissues	1949:1966	intestinal tissues	1949:1966	Moreover, the increased LPS levels and the decreased GLP-2 concentration in plasma and intestinal tissues were observed in 3DG-treated rats, together with the impaired fasting glucose and oral glucose tolerance.
32908918	2	34	from	function	446:453	arg1	rats					511:514	3DG-induced prediabetic rats	487:514	3DG-induced prediabetic rats	487:514	Since the relationship between type 2 diabetes (T2D) and intestinal microbiota is moving from correlation to causality, we investigated the alterations in the composition and function of the intestinal microbiota in 3DG-induced prediabetic rats.
32908918	0	35	theme	3-Deoxyglucosone-Induced	39:62	arg1	Rats					76:79	3-Deoxyglucosone-Induced Prediabetic Rats	39:79	3-Deoxyglucosone-Induced Prediabetic Rats	39:79	Alteration of Intestinal Microbiota in 3-Deoxyglucosone-Induced Prediabetic Rats.
32908918	13	36	theme	prediabetes	2269:2279	arg1	development					2254:2264	the development	2250:2264	the development of prediabetes	2250:2279	The alterations in composition and function of the intestinal microbiota were observed in 3DG-treated rats, which provides a possible mechanism linking exogenous 3DG intake to the development of prediabetes.
32908918	2	37	dep	composition	430:440	arg1	the					426:428	the	426:428	the	426:428	Since the relationship between type 2 diabetes (T2D) and intestinal microbiota is moving from correlation to causality, we investigated the alterations in the composition and function of the intestinal microbiota in 3DG-induced prediabetic rats.
32908918	0	38	theme	Intestinal	14:23	arg1	Microbiota					25:34	Intestinal Microbiota	14:34	Intestinal Microbiota	14:34	Alteration of Intestinal Microbiota in 3-Deoxyglucosone-Induced Prediabetic Rats.
32908918	9	39	theme	Candidatus	1358:1367	arg1	family					1430:1435	Candidatus Soleaferrea genus, Gelria genus, and Thermoanaerobacteraceae family	1358:1435	Candidatus Soleaferrea genus, Gelria genus, and Thermoanaerobacteraceae family	1358:1435	The bacteria of Candidatus Soleaferrea genus, Gelria genus, and Thermoanaerobacteraceae family and its attachment to Thermoanaerobacterales order were apparently more abundant in the control group.
32908918	2	40	theme	intestinal	462:471	arg1	microbiota					473:482	the intestinal microbiota	458:482	the intestinal microbiota	458:482	Since the relationship between type 2 diabetes (T2D) and intestinal microbiota is moving from correlation to causality, we investigated the alterations in the composition and function of the intestinal microbiota in 3DG-induced prediabetic rats.
32908918	8	41	dep	family	1219:1224	arg1	genus					1190:1194	Parasutterela genus and the Alcaligencaeae family and Burkholderiales order	1176:1250	Parasutterela genus and the Alcaligencaeae family and Burkholderiales order	1176:1250	Meanwhile, the abundance of the Prevotellaceae family and Parasutterela genus and the Alcaligencaeae family and Burkholderiales order and its attachment to the Betaproteobacteria class were overrepresented in the 3DG group.
32908918	11	42	theme	acid	1736:1739	arg1	metabolism					1741:1750	amino acid metabolism	1730:1750	amino acid metabolism	1730:1750	Among these KEGG pathways, 13 KEGG pathways were involved in host metabolic function related to amino acid metabolism, carbohydrate metabolism, metabolism of cofactors and vitamins, and metabolism of terpenoids and polyketides.
32908918	12	43	theme	decreased	1905:1913	arg1	concentration					1921:1933	the decreased GLP-2 concentration	1901:1933	the decreased GLP-2 concentration	1901:1933	Moreover, the increased LPS levels and the decreased GLP-2 concentration in plasma and intestinal tissues were observed in 3DG-treated rats, together with the impaired fasting glucose and oral glucose tolerance.
32908918	10	44	theme	KEGG	1556:1559	arg1	pathways					1561:1568	45 KEGG pathways	1553:1568	45 KEGG pathways	1553:1568	In addition, 45 KEGG pathways were altered after two-week intragastric administration of 3DG.
32908918	2	45	theme	intestinal	328:337	arg1	microbiota					339:348	intestinal microbiota	328:348	intestinal microbiota	328:348	Since the relationship between type 2 diabetes (T2D) and intestinal microbiota is moving from correlation to causality, we investigated the alterations in the composition and function of the intestinal microbiota in 3DG-induced prediabetic rats.
32908918	0	46	theme	Prediabetic	64:74	arg1	Rats					76:79	3-Deoxyglucosone-Induced Prediabetic Rats	39:79	3-Deoxyglucosone-Induced Prediabetic Rats	39:79	Alteration of Intestinal Microbiota in 3-Deoxyglucosone-Induced Prediabetic Rats.
32908918	9	47	theme	Gelria	1388:1393	arg1	family					1430:1435	Candidatus Soleaferrea genus, Gelria genus, and Thermoanaerobacteraceae family	1358:1435	Candidatus Soleaferrea genus, Gelria genus, and Thermoanaerobacteraceae family	1358:1435	The bacteria of Candidatus Soleaferrea genus, Gelria genus, and Thermoanaerobacteraceae family and its attachment to Thermoanaerobacterales order were apparently more abundant in the control group.
32908918	1	48	theme	independent	216:226	arg1	3-deoxyglucosone					118:133	3-deoxyglucosone	118:133	3-deoxyglucosone (3DG)	118:139	Our previous research suggests that 3-deoxyglucosone (3DG), formed in the caramelization course and Maillard reactions in food, is an independent factor for the development of prediabetes.
32908918	1	48	theme	independent	216:226	arg1	factor					228:233	an independent factor	213:233	an independent factor for the development of prediabetes	213:268	Our previous research suggests that 3-deoxyglucosone (3DG), formed in the caramelization course and Maillard reactions in food, is an independent factor for the development of prediabetes.
32908918	12	49	theme	LPS	1886:1888	arg1	levels					1890:1895	increased LPS levels	1876:1895	increased LPS levels	1876:1895	Moreover, the increased LPS levels and the decreased GLP-2 concentration in plasma and intestinal tissues were observed in 3DG-treated rats, together with the impaired fasting glucose and oral glucose tolerance.
32908918	12	50	dep	levels	1890:1895	arg1	the					1872:1874	the	1872:1874	the	1872:1874	Moreover, the increased LPS levels and the decreased GLP-2 concentration in plasma and intestinal tissues were observed in 3DG-treated rats, together with the impaired fasting glucose and oral glucose tolerance.
32908918	9	51	theme	Thermoanaerobacteraceae	1406:1428	arg1	family					1430:1435	Candidatus Soleaferrea genus, Gelria genus, and Thermoanaerobacteraceae family	1358:1435	Candidatus Soleaferrea genus, Gelria genus, and Thermoanaerobacteraceae family	1358:1435	The bacteria of Candidatus Soleaferrea genus, Gelria genus, and Thermoanaerobacteraceae family and its attachment to Thermoanaerobacterales order were apparently more abundant in the control group.
32908918	12	52	theme	glucose	2055:2061	arg1	tolerance					2063:2071	oral glucose tolerance	2050:2071	oral glucose tolerance	2050:2071	Moreover, the increased LPS levels and the decreased GLP-2 concentration in plasma and intestinal tissues were observed in 3DG-treated rats, together with the impaired fasting glucose and oral glucose tolerance.
32908918	2	53	from	alterations	411:421	arg1	composition					430:440	composition	430:440	composition	430:440	Since the relationship between type 2 diabetes (T2D) and intestinal microbiota is moving from correlation to causality, we investigated the alterations in the composition and function of the intestinal microbiota in 3DG-induced prediabetic rats.
32908918	2	53	from	alterations	411:421	arg1	function					446:453	function	446:453	function	446:453	Since the relationship between type 2 diabetes (T2D) and intestinal microbiota is moving from correlation to causality, we investigated the alterations in the composition and function of the intestinal microbiota in 3DG-induced prediabetic rats.
32908918	7	54	theme	dominant	1099:1106	arg1	bacteria					1108:1115	the dominant bacteria	1095:1115	the dominant bacteria	1095:1115	At the phylum level, 3DG treatment increased the abundance of nondominant bacteria Proteobacteria but did not cause the change of the dominant bacteria.
32908918	13	55	located	observed	2152:2159	arg1	rats					2176:2179	3DG-treated rats	2164:2179	3DG-treated rats	2164:2179	The alterations in composition and function of the intestinal microbiota were observed in 3DG-treated rats, which provides a possible mechanism linking exogenous 3DG intake to the development of prediabetes.
32908918	13	55	located	observed	2152:2159	arg2	alterations					2078:2088	The alterations	2074:2088	The alterations in composition and function of the intestinal microbiota	2074:2145	The alterations in composition and function of the intestinal microbiota were observed in 3DG-treated rats, which provides a possible mechanism linking exogenous 3DG intake to the development of prediabetes.
32908918	9	56	theme	attachment	1445:1454	arg1	abundant					1509:1516	abundant	1509:1516	abundant	1509:1516	The bacteria of Candidatus Soleaferrea genus, Gelria genus, and Thermoanaerobacteraceae family and its attachment to Thermoanaerobacterales order were apparently more abundant in the control group.
32908918	9	56	theme	attachment	1445:1454	arg1	bacteria					1346:1353	The bacteria	1342:1353	The bacteria of Candidatus Soleaferrea genus, Gelria genus, and Thermoanaerobacteraceae family and its attachment to Thermoanaerobacterales order	1342:1486	The bacteria of Candidatus Soleaferrea genus, Gelria genus, and Thermoanaerobacteraceae family and its attachment to Thermoanaerobacterales order were apparently more abundant in the control group.
32908918	12	57	theme	fasting	2030:2036	arg1	glucose					2038:2044	the impaired fasting glucose	2017:2044	the impaired fasting glucose	2017:2044	Moreover, the increased LPS levels and the decreased GLP-2 concentration in plasma and intestinal tissues were observed in 3DG-treated rats, together with the impaired fasting glucose and oral glucose tolerance.
32908918	4	58	theme	Microbial	592:600	arg1	profiling					602:610	Microbial profiling	592:610	Microbial profiling in faeces samples	592:628	Microbial profiling in faeces samples was determined through the 16S rRNA gene sequence.
32908918	10	59	theme	intragastric	1598:1609	arg1	administration					1611:1624	two-week intragastric administration	1589:1624	two-week intragastric administration of 3DG	1589:1631	In addition, 45 KEGG pathways were altered after two-week intragastric administration of 3DG.
32908918	9	60	theme	Thermoanaerobacterales	1459:1480	arg1	order					1482:1486	Thermoanaerobacterales order	1459:1486	Thermoanaerobacterales order	1459:1486	The bacteria of Candidatus Soleaferrea genus, Gelria genus, and Thermoanaerobacteraceae family and its attachment to Thermoanaerobacterales order were apparently more abundant in the control group.
32908918	8	61	theme	family	1165:1170	arg1	abundance					1133:1141	the abundance	1129:1141	the abundance of the Prevotellaceae family and Parasutterela genus and the Alcaligencaeae family and Burkholderiales order	1129:1250	Meanwhile, the abundance of the Prevotellaceae family and Parasutterela genus and the Alcaligencaeae family and Burkholderiales order and its attachment to the Betaproteobacteria class were overrepresented in the 3DG group.
32908918	8	61	theme	family	1165:1170	arg1	attachment					1260:1269	its attachment	1256:1269	its attachment to the Betaproteobacteria class	1256:1301	Meanwhile, the abundance of the Prevotellaceae family and Parasutterela genus and the Alcaligencaeae family and Burkholderiales order and its attachment to the Betaproteobacteria class were overrepresented in the 3DG group.
32908918	8	61	theme	family	1165:1170	arg1	Meanwhile					1118:1126	Meanwhile	1118:1126	Meanwhile	1118:1126	Meanwhile, the abundance of the Prevotellaceae family and Parasutterela genus and the Alcaligencaeae family and Burkholderiales order and its attachment to the Betaproteobacteria class were overrepresented in the 3DG group.
32908918	4	62	theme	rRNA	661:664	arg1	sequence					671:678	the 16S rRNA gene sequence	653:678	the 16S rRNA gene sequence	653:678	Microbial profiling in faeces samples was determined through the 16S rRNA gene sequence.
32908918	11	63	theme	host	1695:1698	arg1	function					1710:1717	host metabolic function	1695:1717	host metabolic function related to amino acid metabolism, carbohydrate metabolism, metabolism of cofactors and vitamins, and metabolism of terpenoids and polyketides	1695:1859	Among these KEGG pathways, 13 KEGG pathways were involved in host metabolic function related to amino acid metabolism, carbohydrate metabolism, metabolism of cofactors and vitamins, and metabolism of terpenoids and polyketides.
32908918	8	64	theme	Parasutterela	1176:1188	arg1	family					1219:1224	the Alcaligencaeae family	1200:1224	the Alcaligencaeae family	1200:1224	Meanwhile, the abundance of the Prevotellaceae family and Parasutterela genus and the Alcaligencaeae family and Burkholderiales order and its attachment to the Betaproteobacteria class were overrepresented in the 3DG group.
32908918	12	65	located	observed	1973:1980	arg1	rats					1997:2000	3DG-treated rats	1985:2000	3DG-treated rats	1985:2000	Moreover, the increased LPS levels and the decreased GLP-2 concentration in plasma and intestinal tissues were observed in 3DG-treated rats, together with the impaired fasting glucose and oral glucose tolerance.
32908918	12	65	located	observed	1973:1980	arg2	concentration					1921:1933	the decreased GLP-2 concentration	1901:1933	the decreased GLP-2 concentration	1901:1933	Moreover, the increased LPS levels and the decreased GLP-2 concentration in plasma and intestinal tissues were observed in 3DG-treated rats, together with the impaired fasting glucose and oral glucose tolerance.
32908918	12	65	located	observed	1973:1980	arg2	levels					1890:1895	increased LPS levels	1876:1895	increased LPS levels	1876:1895	Moreover, the increased LPS levels and the decreased GLP-2 concentration in plasma and intestinal tissues were observed in 3DG-treated rats, together with the impaired fasting glucose and oral glucose tolerance.
32908918	13	66	from	alterations	2078:2088	arg1	composition					2093:2103	composition	2093:2103	composition	2093:2103	The alterations in composition and function of the intestinal microbiota were observed in 3DG-treated rats, which provides a possible mechanism linking exogenous 3DG intake to the development of prediabetes.
32908918	13	66	from	alterations	2078:2088	arg1	function					2109:2116	function	2109:2116	function	2109:2116	The alterations in composition and function of the intestinal microbiota were observed in 3DG-treated rats, which provides a possible mechanism linking exogenous 3DG intake to the development of prediabetes.
32908918	3	67	theme	intragastric	549:560	arg1	administration					562:575	intragastric administration	549:575	intragastric administration for two weeks	549:589	Rats were given 50 mg/kg 3DG by intragastric administration for two weeks.
32908918	8	68	theme	Alcaligencaeae	1204:1217	arg1	family					1219:1224	the Alcaligencaeae family	1200:1224	the Alcaligencaeae family	1200:1224	Meanwhile, the abundance of the Prevotellaceae family and Parasutterela genus and the Alcaligencaeae family and Burkholderiales order and its attachment to the Betaproteobacteria class were overrepresented in the 3DG group.
32908918	6	69	theme	intestinal	942:951	arg1	microbiota					953:962	intestinal microbiota	942:962	intestinal microbiota	942:962	3DG treatment did not significantly change the richness and evenness but affected the composition of intestinal microbiota.
32908918	8	70	theme	Betaproteobacteria	1278:1295	arg1	class					1297:1301	the Betaproteobacteria class	1274:1301	the Betaproteobacteria class	1274:1301	Meanwhile, the abundance of the Prevotellaceae family and Parasutterela genus and the Alcaligencaeae family and Burkholderiales order and its attachment to the Betaproteobacteria class were overrepresented in the 3DG group.
32908918	5	71	theme	lipopolysaccharide	721:738	arg1	levels					746:751	The glucagon-like peptide 2 (GLP-2) and lipopolysaccharide (LPS) levels	681:751	The glucagon-like peptide 2 (GLP-2) and lipopolysaccharide (LPS) levels in plasma and intestinal tissues	681:784	The glucagon-like peptide 2 (GLP-2) and lipopolysaccharide (LPS) levels in plasma and intestinal tissues were measured by ELISA and Limulus test, respectively.
32908918	7	72	theme	bacteria	1039:1046	arg1	Proteobacteria					1048:1061	nondominant bacteria Proteobacteria	1027:1061	nondominant bacteria Proteobacteria	1027:1061	At the phylum level, 3DG treatment increased the abundance of nondominant bacteria Proteobacteria but did not cause the change of the dominant bacteria.
32908918	9	73	theme	control	1525:1531	arg1	group					1533:1537	the control group	1521:1537	the control group	1521:1537	The bacteria of Candidatus Soleaferrea genus, Gelria genus, and Thermoanaerobacteraceae family and its attachment to Thermoanaerobacterales order were apparently more abundant in the control group.
32908918	0	74	from	Alteration	0:9	arg1	Rats					76:79	3-Deoxyglucosone-Induced Prediabetic Rats	39:79	3-Deoxyglucosone-Induced Prediabetic Rats	39:79	Alteration of Intestinal Microbiota in 3-Deoxyglucosone-Induced Prediabetic Rats.
32908918	5	75	theme	peptide	699:705	arg1	levels					746:751	The glucagon-like peptide 2 (GLP-2) and lipopolysaccharide (LPS) levels	681:751	The glucagon-like peptide 2 (GLP-2) and lipopolysaccharide (LPS) levels in plasma and intestinal tissues	681:784	The glucagon-like peptide 2 (GLP-2) and lipopolysaccharide (LPS) levels in plasma and intestinal tissues were measured by ELISA and Limulus test, respectively.
32908918	11	76	theme	cofactors	1792:1800	arg1	metabolism					1820:1829	metabolism	1820:1829	metabolism of terpenoids and polyketides	1820:1859	Among these KEGG pathways, 13 KEGG pathways were involved in host metabolic function related to amino acid metabolism, carbohydrate metabolism, metabolism of cofactors and vitamins, and metabolism of terpenoids and polyketides.
32908918	11	76	theme	cofactors	1792:1800	arg1	metabolism					1778:1787	metabolism	1778:1787	metabolism of cofactors and vitamins	1778:1813	Among these KEGG pathways, 13 KEGG pathways were involved in host metabolic function related to amino acid metabolism, carbohydrate metabolism, metabolism of cofactors and vitamins, and metabolism of terpenoids and polyketides.
32908918	11	76	theme	cofactors	1792:1800	arg1	metabolism					1741:1750	amino acid metabolism	1730:1750	amino acid metabolism	1730:1750	Among these KEGG pathways, 13 KEGG pathways were involved in host metabolic function related to amino acid metabolism, carbohydrate metabolism, metabolism of cofactors and vitamins, and metabolism of terpenoids and polyketides.
32908918	11	76	theme	cofactors	1792:1800	arg1	metabolism					1766:1775	carbohydrate metabolism	1753:1775	carbohydrate metabolism	1753:1775	Among these KEGG pathways, 13 KEGG pathways were involved in host metabolic function related to amino acid metabolism, carbohydrate metabolism, metabolism of cofactors and vitamins, and metabolism of terpenoids and polyketides.
32908918	7	77	theme	3DG	986:988	arg1	treatment					990:998	3DG treatment	986:998	3DG treatment	986:998	At the phylum level, 3DG treatment increased the abundance of nondominant bacteria Proteobacteria but did not cause the change of the dominant bacteria.
32908918	2	78	theme	3DG-induced	487:497	arg1	rats					511:514	3DG-induced prediabetic rats	487:514	3DG-induced prediabetic rats	487:514	Since the relationship between type 2 diabetes (T2D) and intestinal microbiota is moving from correlation to causality, we investigated the alterations in the composition and function of the intestinal microbiota in 3DG-induced prediabetic rats.
32908918	9	79	dep	Candidatus	1358:1367	arg1	genus					1381:1385	Candidatus Soleaferrea genus	1358:1385	Candidatus Soleaferrea genus	1358:1385	The bacteria of Candidatus Soleaferrea genus, Gelria genus, and Thermoanaerobacteraceae family and its attachment to Thermoanaerobacterales order were apparently more abundant in the control group.
32908918	8	80	theme	3DG	1331:1333	arg1	group					1335:1339	the 3DG group	1327:1339	the 3DG group	1327:1339	Meanwhile, the abundance of the Prevotellaceae family and Parasutterela genus and the Alcaligencaeae family and Burkholderiales order and its attachment to the Betaproteobacteria class were overrepresented in the 3DG group.
32908918	1	81	theme	caramelization	156:169	arg1	course					171:176	caramelization course	156:176	caramelization course	156:176	Our previous research suggests that 3-deoxyglucosone (3DG), formed in the caramelization course and Maillard reactions in food, is an independent factor for the development of prediabetes.
32908918	2	82	theme	microbiota	473:482	arg1	composition					430:440	composition	430:440	composition	430:440	Since the relationship between type 2 diabetes (T2D) and intestinal microbiota is moving from correlation to causality, we investigated the alterations in the composition and function of the intestinal microbiota in 3DG-induced prediabetic rats.
32908918	2	82	theme	microbiota	473:482	arg1	function					446:453	function	446:453	function	446:453	Since the relationship between type 2 diabetes (T2D) and intestinal microbiota is moving from correlation to causality, we investigated the alterations in the composition and function of the intestinal microbiota in 3DG-induced prediabetic rats.
32908918	4	83	from	profiling	602:610	arg1	samples					622:628	faeces samples	615:628	faeces samples	615:628	Microbial profiling in faeces samples was determined through the 16S rRNA gene sequence.
32908918	13	84	theme	intestinal	2125:2134	arg1	microbiota					2136:2145	the intestinal microbiota	2121:2145	the intestinal microbiota	2121:2145	The alterations in composition and function of the intestinal microbiota were observed in 3DG-treated rats, which provides a possible mechanism linking exogenous 3DG intake to the development of prediabetes.
32908918	1	85	from	course	171:176	arg1	food					204:207	food	204:207	food	204:207	Our previous research suggests that 3-deoxyglucosone (3DG), formed in the caramelization course and Maillard reactions in food, is an independent factor for the development of prediabetes.
32908918	9	86	dep	Gelria	1388:1393	arg1	genus					1395:1399	Gelria genus	1388:1399	Gelria genus	1388:1399	The bacteria of Candidatus Soleaferrea genus, Gelria genus, and Thermoanaerobacteraceae family and its attachment to Thermoanaerobacterales order were apparently more abundant in the control group.
32908918	11	87	theme	vitamins	1806:1813	arg1	metabolism					1820:1829	metabolism	1820:1829	metabolism of terpenoids and polyketides	1820:1859	Among these KEGG pathways, 13 KEGG pathways were involved in host metabolic function related to amino acid metabolism, carbohydrate metabolism, metabolism of cofactors and vitamins, and metabolism of terpenoids and polyketides.
32908918	11	87	theme	vitamins	1806:1813	arg1	metabolism					1778:1787	metabolism	1778:1787	metabolism of cofactors and vitamins	1778:1813	Among these KEGG pathways, 13 KEGG pathways were involved in host metabolic function related to amino acid metabolism, carbohydrate metabolism, metabolism of cofactors and vitamins, and metabolism of terpenoids and polyketides.
32908918	11	87	theme	vitamins	1806:1813	arg1	metabolism					1741:1750	amino acid metabolism	1730:1750	amino acid metabolism	1730:1750	Among these KEGG pathways, 13 KEGG pathways were involved in host metabolic function related to amino acid metabolism, carbohydrate metabolism, metabolism of cofactors and vitamins, and metabolism of terpenoids and polyketides.
32908918	11	87	theme	vitamins	1806:1813	arg1	metabolism					1766:1775	carbohydrate metabolism	1753:1775	carbohydrate metabolism	1753:1775	Among these KEGG pathways, 13 KEGG pathways were involved in host metabolic function related to amino acid metabolism, carbohydrate metabolism, metabolism of cofactors and vitamins, and metabolism of terpenoids and polyketides.
32908918	1	88	theme	previous	86:93	arg1	research					95:102	Our previous research	82:102	Our previous research	82:102	Our previous research suggests that 3-deoxyglucosone (3DG), formed in the caramelization course and Maillard reactions in food, is an independent factor for the development of prediabetes.
32908918	12	89	theme	3DG-treated	1985:1995	arg1	rats					1997:2000	3DG-treated rats	1985:2000	3DG-treated rats	1985:2000	Moreover, the increased LPS levels and the decreased GLP-2 concentration in plasma and intestinal tissues were observed in 3DG-treated rats, together with the impaired fasting glucose and oral glucose tolerance.
32908918	1	90	dep	course	171:176	arg1	the					152:154	the	152:154	the	152:154	Our previous research suggests that 3-deoxyglucosone (3DG), formed in the caramelization course and Maillard reactions in food, is an independent factor for the development of prediabetes.
32908918	0	91	theme	Microbiota	25:34	arg1	Alteration					0:9	Alteration	0:9	Alteration of Intestinal Microbiota in 3-Deoxyglucosone-Induced Prediabetic Rats.	0:80	Alteration of Intestinal Microbiota in 3-Deoxyglucosone-Induced Prediabetic Rats.
32908918	11	92	theme	amino	1730:1734	arg1	metabolism					1741:1750	amino acid metabolism	1730:1750	amino acid metabolism	1730:1750	Among these KEGG pathways, 13 KEGG pathways were involved in host metabolic function related to amino acid metabolism, carbohydrate metabolism, metabolism of cofactors and vitamins, and metabolism of terpenoids and polyketides.
32908918	5	93	theme	intestinal	767:776	arg1	tissues					778:784	intestinal tissues	767:784	intestinal tissues	767:784	The glucagon-like peptide 2 (GLP-2) and lipopolysaccharide (LPS) levels in plasma and intestinal tissues were measured by ELISA and Limulus test, respectively.
32908918	12	94	theme	GLP-2	1915:1919	arg1	concentration					1921:1933	the decreased GLP-2 concentration	1901:1933	the decreased GLP-2 concentration	1901:1933	Moreover, the increased LPS levels and the decreased GLP-2 concentration in plasma and intestinal tissues were observed in 3DG-treated rats, together with the impaired fasting glucose and oral glucose tolerance.
32908918	11	95	theme	related	1719:1725	arg1	function					1710:1717	host metabolic function	1695:1717	host metabolic function related to amino acid metabolism, carbohydrate metabolism, metabolism of cofactors and vitamins, and metabolism of terpenoids and polyketides	1695:1859	Among these KEGG pathways, 13 KEGG pathways were involved in host metabolic function related to amino acid metabolism, carbohydrate metabolism, metabolism of cofactors and vitamins, and metabolism of terpenoids and polyketides.
32908918	13	96	theme	3DG	2236:2238	arg1	intake					2240:2245	exogenous 3DG intake	2226:2245	exogenous 3DG intake	2226:2245	The alterations in composition and function of the intestinal microbiota were observed in 3DG-treated rats, which provides a possible mechanism linking exogenous 3DG intake to the development of prediabetes.
32908918	12	97	from	levels	1890:1895	arg1	plasma					1938:1943	plasma	1938:1943	plasma	1938:1943	Moreover, the increased LPS levels and the decreased GLP-2 concentration in plasma and intestinal tissues were observed in 3DG-treated rats, together with the impaired fasting glucose and oral glucose tolerance.
32908918	12	97	from	levels	1890:1895	arg1	tissues					1960:1966	intestinal tissues	1949:1966	intestinal tissues	1949:1966	Moreover, the increased LPS levels and the decreased GLP-2 concentration in plasma and intestinal tissues were observed in 3DG-treated rats, together with the impaired fasting glucose and oral glucose tolerance.
32908918	12	98	theme	increased	1876:1884	arg1	levels					1890:1895	increased LPS levels	1876:1895	increased LPS levels	1876:1895	Moreover, the increased LPS levels and the decreased GLP-2 concentration in plasma and intestinal tissues were observed in 3DG-treated rats, together with the impaired fasting glucose and oral glucose tolerance.
32908918	6	99	theme	3DG	841:843	arg1	treatment					845:853	3DG treatment	841:853	3DG treatment	841:853	3DG treatment did not significantly change the richness and evenness but affected the composition of intestinal microbiota.
32908918	11	100	theme	carbohydrate	1753:1764	arg1	metabolism					1766:1775	carbohydrate metabolism	1753:1775	carbohydrate metabolism	1753:1775	Among these KEGG pathways, 13 KEGG pathways were involved in host metabolic function related to amino acid metabolism, carbohydrate metabolism, metabolism of cofactors and vitamins, and metabolism of terpenoids and polyketides.
32908918	9	101	from	abundant	1509:1516	arg1	group					1533:1537	the control group	1521:1537	the control group	1521:1537	The bacteria of Candidatus Soleaferrea genus, Gelria genus, and Thermoanaerobacteraceae family and its attachment to Thermoanaerobacterales order were apparently more abundant in the control group.
32908918	4	102	theme	faeces	615:620	arg1	samples					622:628	faeces samples	615:628	faeces samples	615:628	Microbial profiling in faeces samples was determined through the 16S rRNA gene sequence.
32908918	13	103	theme	exogenous	2226:2234	arg1	intake					2240:2245	exogenous 3DG intake	2226:2245	exogenous 3DG intake	2226:2245	The alterations in composition and function of the intestinal microbiota were observed in 3DG-treated rats, which provides a possible mechanism linking exogenous 3DG intake to the development of prediabetes.
32908918	13	104	theme	possible	2199:2206	arg1	mechanism					2208:2216	a possible mechanism	2197:2216	a possible mechanism linking exogenous 3DG intake to the development of prediabetes	2197:2279	The alterations in composition and function of the intestinal microbiota were observed in 3DG-treated rats, which provides a possible mechanism linking exogenous 3DG intake to the development of prediabetes.
32908918	11	105	theme	KEGG	1646:1649	arg1	pathways					1651:1658	these KEGG pathways	1640:1658	these KEGG pathways	1640:1658	Among these KEGG pathways, 13 KEGG pathways were involved in host metabolic function related to amino acid metabolism, carbohydrate metabolism, metabolism of cofactors and vitamins, and metabolism of terpenoids and polyketides.
32908918	9	106	theme	family	1430:1435	arg1	abundant					1509:1516	abundant	1509:1516	abundant	1509:1516	The bacteria of Candidatus Soleaferrea genus, Gelria genus, and Thermoanaerobacteraceae family and its attachment to Thermoanaerobacterales order were apparently more abundant in the control group.
32908918	9	106	theme	family	1430:1435	arg1	bacteria					1346:1353	The bacteria	1342:1353	The bacteria of Candidatus Soleaferrea genus, Gelria genus, and Thermoanaerobacteraceae family and its attachment to Thermoanaerobacterales order	1342:1486	The bacteria of Candidatus Soleaferrea genus, Gelria genus, and Thermoanaerobacteraceae family and its attachment to Thermoanaerobacterales order were apparently more abundant in the control group.
32204005	6	0	theme	covalent	1021:1028	arg1	binding					1030:1036	covalent binding	1021:1036	covalent binding	1021:1036	Furthermore, long-term antibacterial property up to 3 weeks against both gram-positive Staphylococcus aureus (S. aureus) and gram-negative Escherichia coli (E. coli) was obtained combined with the merits of covalent binding and LBL methods.
32204005	7	1	theme	titanium	1132:1139	arg1	dioxide					1141:1147	titanium dioxide	1132:1147	titanium dioxide nanotubes (TNTs) modified with composite films	1132:1194	Meanwhile, the cell proliferation and osteogenic differentiation of BMSCs on titanium dioxide nanotubes (TNTs) modified with composite films was significantly improved.
32204005	7	2	from	nanotubes	1149:1157	arg1	differentiation					1104:1118	osteogenic differentiation	1093:1118	osteogenic differentiation	1093:1118	Meanwhile, the cell proliferation and osteogenic differentiation of BMSCs on titanium dioxide nanotubes (TNTs) modified with composite films was significantly improved.
32204005	7	2	from	nanotubes	1149:1157	arg1	Meanwhile					1055:1063	Meanwhile	1055:1063	Meanwhile	1055:1063	Meanwhile, the cell proliferation and osteogenic differentiation of BMSCs on titanium dioxide nanotubes (TNTs) modified with composite films was significantly improved.
32204005	7	2	from	nanotubes	1149:1157	arg1	proliferation					1075:1087	cell proliferation	1070:1087	cell proliferation	1070:1087	Meanwhile, the cell proliferation and osteogenic differentiation of BMSCs on titanium dioxide nanotubes (TNTs) modified with composite films was significantly improved.
32204005	4	3	theme	gum	531:533	arg1	films					563:567	ε-polylysine and gum Arabic multilayer composite films	514:567	ε-polylysine and gum Arabic multilayer composite films	514:567	In this work, ε-polylysine and gum Arabic multilayer composite films were immobilized layer by layer (LBL) on anodized titanium with the assistance of polydopamine for the first time.
32204005	7	4	theme	dioxide	1141:1147	arg1	TNTs					1160:1163	TNTs	1160:1163	TNTs	1160:1163	Meanwhile, the cell proliferation and osteogenic differentiation of BMSCs on titanium dioxide nanotubes (TNTs) modified with composite films was significantly improved.
32204005	7	4	theme	dioxide	1141:1147	arg1	nanotubes					1149:1157	titanium dioxide nanotubes	1132:1157	titanium dioxide nanotubes (TNTs) modified with composite films	1132:1194	Meanwhile, the cell proliferation and osteogenic differentiation of BMSCs on titanium dioxide nanotubes (TNTs) modified with composite films was significantly improved.
32204005	6	5	theme	long-term	827:835	arg1	property					851:858	long-term antibacterial property	827:858	long-term antibacterial property up to 3 weeks against both gram-positive Staphylococcus aureus (S. aureus) and gram-negative Escherichia coli (E. coli)	827:978	Furthermore, long-term antibacterial property up to 3 weeks against both gram-positive Staphylococcus aureus (S. aureus) and gram-negative Escherichia coli (E. coli) was obtained combined with the merits of covalent binding and LBL methods.
32204005	1	6	theme	crucial	181:187	arg1	reason					189:194	a crucial reason	179:194	a crucial reason that give rise to failure of medical implants in clinical applications	179:265	Bacterial infection has become a crucial reason that give rise to failure of medical implants in clinical applications.
32204005	0	7	theme	antibacterial	107:119	arg1	properties					136:145	antibacterial and osteogenic properties	107:145	antibacterial and osteogenic properties	107:145	Layer-by-layer immobilizing of polydopamine-assisted ε-polylysine and gum Arabic on titanium: Tailoring of antibacterial and osteogenic properties.
32204005	7	8	theme	BMSCs	1123:1127	arg1	differentiation					1104:1118	osteogenic differentiation	1093:1118	osteogenic differentiation	1093:1118	Meanwhile, the cell proliferation and osteogenic differentiation of BMSCs on titanium dioxide nanotubes (TNTs) modified with composite films was significantly improved.
32204005	7	8	theme	BMSCs	1123:1127	arg1	Meanwhile					1055:1063	Meanwhile	1055:1063	Meanwhile	1055:1063	Meanwhile, the cell proliferation and osteogenic differentiation of BMSCs on titanium dioxide nanotubes (TNTs) modified with composite films was significantly improved.
32204005	7	8	theme	BMSCs	1123:1127	arg1	proliferation					1075:1087	cell proliferation	1070:1087	cell proliferation	1070:1087	Meanwhile, the cell proliferation and osteogenic differentiation of BMSCs on titanium dioxide nanotubes (TNTs) modified with composite films was significantly improved.
32204005	2	9	theme	surface	306:312	arg1	modifications					314:326	various antibacterial surface modifications	284:326	various antibacterial surface modifications of implants	284:338	In this regard, various antibacterial surface modifications of implants have been developed in recent years.
32204005	8	10	dep	method	1245:1250	arg1	optimize					1255:1262	optimize	1255:1262	to optimize anti-infective and osteogenic properties of medical titanium	1252:1323	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	8	11	dep	materials	1507:1515	arg1	property					1545:1552	improved antibacterial property	1522:1552	improved antibacterial property	1522:1552	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	8	11	dep	materials	1507:1515	arg1	biocompatibility					1568:1583	excellent biocompatibility	1558:1583	excellent biocompatibility	1558:1583	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	8	11	dep	materials	1507:1515	arg1	materials					1507:1515	the orthopedic implant materials	1484:1515	the orthopedic implant materials both improved antibacterial property and excellent biocompatibility	1484:1583	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	2	12	theme	antibacterial	292:304	arg1	modifications					314:326	various antibacterial surface modifications	284:326	various antibacterial surface modifications of implants	284:338	In this regard, various antibacterial surface modifications of implants have been developed in recent years.
32204005	7	13	from	proliferation	1075:1087	arg1	TNTs					1160:1163	TNTs	1160:1163	TNTs	1160:1163	Meanwhile, the cell proliferation and osteogenic differentiation of BMSCs on titanium dioxide nanotubes (TNTs) modified with composite films was significantly improved.
32204005	7	13	from	proliferation	1075:1087	arg1	nanotubes					1149:1157	titanium dioxide nanotubes	1132:1157	titanium dioxide nanotubes (TNTs) modified with composite films	1132:1194	Meanwhile, the cell proliferation and osteogenic differentiation of BMSCs on titanium dioxide nanotubes (TNTs) modified with composite films was significantly improved.
32204005	0	14	theme	osteogenic	125:134	arg1	properties					136:145	antibacterial and osteogenic properties	107:145	antibacterial and osteogenic properties	107:145	Layer-by-layer immobilizing of polydopamine-assisted ε-polylysine and gum Arabic on titanium: Tailoring of antibacterial and osteogenic properties.
32204005	8	15	theme	titanium	1316:1323	arg1	properties					1294:1303	anti-infective and osteogenic properties	1264:1303	anti-infective and osteogenic properties of medical titanium	1264:1323	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	2	16	theme	various	284:290	arg1	modifications					314:326	various antibacterial surface modifications	284:326	various antibacterial surface modifications of implants	284:338	In this regard, various antibacterial surface modifications of implants have been developed in recent years.
32204005	5	17	dep	In	684:685	arg1	vitro					687:691	vitro	687:691	vitro	687:691	In vitro antibacterial results showed that the bacteria numbers decreased with an increase in the loading amount of ε-polylysine.
32204005	2	18	mod	modifications	314:326	arg1	implants					331:338	implants	331:338	implants	331:338	In this regard, various antibacterial surface modifications of implants have been developed in recent years.
32204005	2	18	mod	modifications	314:326	arg3	surface					306:312	various antibacterial surface modifications	284:326	various antibacterial surface modifications of implants	284:338	In this regard, various antibacterial surface modifications of implants have been developed in recent years.
32204005	8	19	theme	titanium-based	1649:1662	arg1	implants					1664:1671	titanium-based implants	1649:1671	titanium-based implants	1649:1671	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	8	20	theme	practical	1624:1632	arg1	application					1634:1644	practical application	1624:1644	practical application of titanium-based implants	1624:1671	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	3	21	theme	suitable	450:457	arg1	properties					488:497	both suitable antibacterial and osteogenic properties	445:497	both suitable antibacterial and osteogenic properties	445:497	However, it remains a challenge to enable the implant surfaces with both suitable antibacterial and osteogenic properties.
32204005	7	22	theme	composite	1180:1188	arg1	films					1190:1194	composite films	1180:1194	composite films	1180:1194	Meanwhile, the cell proliferation and osteogenic differentiation of BMSCs on titanium dioxide nanotubes (TNTs) modified with composite films was significantly improved.
32204005	6	23	theme	LBL	1042:1044	arg1	methods					1046:1052	LBL methods	1042:1052	LBL methods	1042:1052	Furthermore, long-term antibacterial property up to 3 weeks against both gram-positive Staphylococcus aureus (S. aureus) and gram-negative Escherichia coli (E. coli) was obtained combined with the merits of covalent binding and LBL methods.
32204005	8	24	theme	multilayer	1406:1415	arg1	films					1427:1431	the ε-polylysine and gum Arabic multilayer composite films	1374:1431	the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine	1374:1463	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	8	24	theme	multilayer	1406:1415	arg1	able					1470:1473	able	1470:1473	able	1470:1473	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	8	25	theme	excellent	1558:1566	arg1	biocompatibility					1568:1583	excellent biocompatibility	1558:1583	excellent biocompatibility	1558:1583	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	8	25	theme	excellent	1558:1566	arg1	materials					1507:1515	the orthopedic implant materials	1484:1515	the orthopedic implant materials both improved antibacterial property and excellent biocompatibility	1484:1583	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	8	26	theme	anti-infective	1264:1277	arg1	properties					1294:1303	anti-infective and osteogenic properties	1264:1303	anti-infective and osteogenic properties of medical titanium	1264:1323	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	6	27	dep	aureus	916:921	arg1	aureus					927:932	S. aureus	924:932	S. aureus	924:932	Furthermore, long-term antibacterial property up to 3 weeks against both gram-positive Staphylococcus aureus (S. aureus) and gram-negative Escherichia coli (E. coli) was obtained combined with the merits of covalent binding and LBL methods.
32204005	8	28	theme	profound	1598:1605	arg1	significance					1607:1618	profound significance	1598:1618	profound significance	1598:1618	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	6	29	theme	antibacterial	837:849	arg1	property					851:858	long-term antibacterial property	827:858	long-term antibacterial property up to 3 weeks against both gram-positive Staphylococcus aureus (S. aureus) and gram-negative Escherichia coli (E. coli)	827:978	Furthermore, long-term antibacterial property up to 3 weeks against both gram-positive Staphylococcus aureus (S. aureus) and gram-negative Escherichia coli (E. coli) was obtained combined with the merits of covalent binding and LBL methods.
32204005	2	30	theme	implants	331:338	arg1	modifications					314:326	various antibacterial surface modifications	284:326	various antibacterial surface modifications of implants	284:338	In this regard, various antibacterial surface modifications of implants have been developed in recent years.
32204005	5	31	theme	bacteria	731:738	arg1	numbers					740:746	the bacteria numbers	727:746	the bacteria numbers	727:746	In vitro antibacterial results showed that the bacteria numbers decreased with an increase in the loading amount of ε-polylysine.
32204005	4	32	theme	first	672:676	arg1	time					678:681	the first time	668:681	the first time	668:681	In this work, ε-polylysine and gum Arabic multilayer composite films were immobilized layer by layer (LBL) on anodized titanium with the assistance of polydopamine for the first time.
32204005	0	33	theme	polydopamine-assisted	31:51	arg1	ε-polylysine					53:64	polydopamine-assisted ε-polylysine	31:64	polydopamine-assisted ε-polylysine	31:64	Layer-by-layer immobilizing of polydopamine-assisted ε-polylysine and gum Arabic on titanium: Tailoring of antibacterial and osteogenic properties.
32204005	8	34	with	films	1427:1431	arg1	assistance					1438:1447	assistance	1438:1447	assistance	1438:1447	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	7	35	theme	cell	1070:1073	arg1	Meanwhile					1055:1063	Meanwhile	1055:1063	Meanwhile	1055:1063	Meanwhile, the cell proliferation and osteogenic differentiation of BMSCs on titanium dioxide nanotubes (TNTs) modified with composite films was significantly improved.
32204005	7	35	theme	cell	1070:1073	arg1	proliferation					1075:1087	cell proliferation	1070:1087	cell proliferation	1070:1087	Meanwhile, the cell proliferation and osteogenic differentiation of BMSCs on titanium dioxide nanotubes (TNTs) modified with composite films was significantly improved.
32204005	6	36	theme	methods	1046:1052	arg1	merits					1011:1016	the merits	1007:1016	the merits of covalent binding and LBL methods	1007:1052	Furthermore, long-term antibacterial property up to 3 weeks against both gram-positive Staphylococcus aureus (S. aureus) and gram-negative Escherichia coli (E. coli) was obtained combined with the merits of covalent binding and LBL methods.
32204005	8	37	theme	antibacterial	1531:1543	arg1	property					1545:1552	improved antibacterial property	1522:1552	improved antibacterial property	1522:1552	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	8	37	theme	antibacterial	1531:1543	arg1	materials					1507:1515	the orthopedic implant materials	1484:1515	the orthopedic implant materials both improved antibacterial property and excellent biocompatibility	1484:1583	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	3	38	theme	osteogenic	477:486	arg1	properties					488:497	both suitable antibacterial and osteogenic properties	445:497	both suitable antibacterial and osteogenic properties	445:497	However, it remains a challenge to enable the implant surfaces with both suitable antibacterial and osteogenic properties.
32204005	8	39	theme	ε-polylysine	1378:1389	arg1	films					1427:1431	the ε-polylysine and gum Arabic multilayer composite films	1374:1431	the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine	1374:1463	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	8	39	theme	ε-polylysine	1378:1389	arg1	able					1470:1473	able	1470:1473	able	1470:1473	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	7	40	from	differentiation	1104:1118	arg1	TNTs					1160:1163	TNTs	1160:1163	TNTs	1160:1163	Meanwhile, the cell proliferation and osteogenic differentiation of BMSCs on titanium dioxide nanotubes (TNTs) modified with composite films was significantly improved.
32204005	7	40	from	differentiation	1104:1118	arg1	nanotubes					1149:1157	titanium dioxide nanotubes	1132:1157	titanium dioxide nanotubes (TNTs) modified with composite films	1132:1194	Meanwhile, the cell proliferation and osteogenic differentiation of BMSCs on titanium dioxide nanotubes (TNTs) modified with composite films was significantly improved.
32204005	0	41	theme	properties	136:145	arg1	Tailoring					94:102	titanium: Tailoring	84:102	titanium: Tailoring of antibacterial and osteogenic properties	84:145	Layer-by-layer immobilizing of polydopamine-assisted ε-polylysine and gum Arabic on titanium: Tailoring of antibacterial and osteogenic properties.
32204005	6	42	dep	coli	965:968	arg1	coli					974:977	E. coli	971:977	E. coli	971:977	Furthermore, long-term antibacterial property up to 3 weeks against both gram-positive Staphylococcus aureus (S. aureus) and gram-negative Escherichia coli (E. coli) was obtained combined with the merits of covalent binding and LBL methods.
32204005	8	43	theme	gum	1395:1397	arg1	films					1427:1431	the ε-polylysine and gum Arabic multilayer composite films	1374:1431	the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine	1374:1463	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	8	43	theme	gum	1395:1397	arg1	able					1470:1473	able	1470:1473	able	1470:1473	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	7	44	theme	osteogenic	1093:1102	arg1	differentiation					1104:1118	osteogenic differentiation	1093:1118	osteogenic differentiation	1093:1118	Meanwhile, the cell proliferation and osteogenic differentiation of BMSCs on titanium dioxide nanotubes (TNTs) modified with composite films was significantly improved.
32204005	7	44	theme	osteogenic	1093:1102	arg1	Meanwhile					1055:1063	Meanwhile	1055:1063	Meanwhile	1055:1063	Meanwhile, the cell proliferation and osteogenic differentiation of BMSCs on titanium dioxide nanotubes (TNTs) modified with composite films was significantly improved.
32204005	5	45	theme	loading	782:788	arg1	ε-polylysine					800:811	ε-polylysine	800:811	ε-polylysine	800:811	In vitro antibacterial results showed that the bacteria numbers decreased with an increase in the loading amount of ε-polylysine.
32204005	5	45	theme	loading	782:788	arg1	amount					790:795	the loading amount	778:795	the loading amount of ε-polylysine	778:811	In vitro antibacterial results showed that the bacteria numbers decreased with an increase in the loading amount of ε-polylysine.
32204005	8	46	theme	medical	1308:1314	arg1	titanium					1316:1323	medical titanium	1308:1323	medical titanium	1308:1323	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	4	47	theme	polydopamine	651:662	arg1	assistance					637:646	the assistance	633:646	the assistance of polydopamine	633:662	In this work, ε-polylysine and gum Arabic multilayer composite films were immobilized layer by layer (LBL) on anodized titanium with the assistance of polydopamine for the first time.
32204005	8	48	theme	composite	1417:1425	arg1	films					1427:1431	the ε-polylysine and gum Arabic multilayer composite films	1374:1431	the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine	1374:1463	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	8	48	theme	composite	1417:1425	arg1	able					1470:1473	able	1470:1473	able	1470:1473	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	3	49	theme	antibacterial	459:471	arg1	properties					488:497	both suitable antibacterial and osteogenic properties	445:497	both suitable antibacterial and osteogenic properties	445:497	However, it remains a challenge to enable the implant surfaces with both suitable antibacterial and osteogenic properties.
32204005	3	50	theme	implant	423:429	arg1	surfaces					431:438	the implant surfaces	419:438	the implant surfaces with both suitable antibacterial and osteogenic properties	419:497	However, it remains a challenge to enable the implant surfaces with both suitable antibacterial and osteogenic properties.
32204005	4	51	theme	multilayer	542:551	arg1	films					563:567	ε-polylysine and gum Arabic multilayer composite films	514:567	ε-polylysine and gum Arabic multilayer composite films	514:567	In this work, ε-polylysine and gum Arabic multilayer composite films were immobilized layer by layer (LBL) on anodized titanium with the assistance of polydopamine for the first time.
32204005	4	52	with	titanium	619:626	arg1	assistance					637:646	the assistance	633:646	the assistance of polydopamine	633:662	In this work, ε-polylysine and gum Arabic multilayer composite films were immobilized layer by layer (LBL) on anodized titanium with the assistance of polydopamine for the first time.
32204005	1	53	theme	medical	225:231	arg1	implants					233:240	medical implants	225:240	medical implants	225:240	Bacterial infection has become a crucial reason that give rise to failure of medical implants in clinical applications.
32204005	0	54	theme	Arabic	74:79	arg1	ε-polylysine					53:64	polydopamine-assisted ε-polylysine	31:64	polydopamine-assisted ε-polylysine	31:64	Layer-by-layer immobilizing of polydopamine-assisted ε-polylysine and gum Arabic on titanium: Tailoring of antibacterial and osteogenic properties.
32204005	4	55	theme	Arabic	535:540	arg1	films					563:567	ε-polylysine and gum Arabic multilayer composite films	514:567	ε-polylysine and gum Arabic multilayer composite films	514:567	In this work, ε-polylysine and gum Arabic multilayer composite films were immobilized layer by layer (LBL) on anodized titanium with the assistance of polydopamine for the first time.
32204005	3	56	with	surfaces	431:438	arg1	properties					488:497	both suitable antibacterial and osteogenic properties	445:497	both suitable antibacterial and osteogenic properties	445:497	However, it remains a challenge to enable the implant surfaces with both suitable antibacterial and osteogenic properties.
32204005	7	57	from	BMSCs	1123:1127	arg1	TNTs					1160:1163	TNTs	1160:1163	TNTs	1160:1163	Meanwhile, the cell proliferation and osteogenic differentiation of BMSCs on titanium dioxide nanotubes (TNTs) modified with composite films was significantly improved.
32204005	7	57	from	BMSCs	1123:1127	arg1	nanotubes					1149:1157	titanium dioxide nanotubes	1132:1157	titanium dioxide nanotubes (TNTs) modified with composite films	1132:1194	Meanwhile, the cell proliferation and osteogenic differentiation of BMSCs on titanium dioxide nanotubes (TNTs) modified with composite films was significantly improved.
32204005	8	58	theme	improved	1522:1529	arg1	property					1545:1552	improved antibacterial property	1522:1552	improved antibacterial property	1522:1552	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	8	58	theme	improved	1522:1529	arg1	materials					1507:1515	the orthopedic implant materials	1484:1515	the orthopedic implant materials both improved antibacterial property and excellent biocompatibility	1484:1583	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	2	59	theme	recent	363:368	arg1	years					370:374	recent years	363:374	recent years	363:374	In this regard, various antibacterial surface modifications of implants have been developed in recent years.
32204005	5	60	theme	ε-polylysine	800:811	arg1	ε-polylysine					800:811	ε-polylysine	800:811	ε-polylysine	800:811	In vitro antibacterial results showed that the bacteria numbers decreased with an increase in the loading amount of ε-polylysine.
32204005	5	60	theme	ε-polylysine	800:811	arg1	amount					790:795	the loading amount	778:795	the loading amount of ε-polylysine	778:811	In vitro antibacterial results showed that the bacteria numbers decreased with an increase in the loading amount of ε-polylysine.
32204005	5	61	theme	In	684:685	arg1	results					707:713	In vitro antibacterial results	684:713	In vitro antibacterial results	684:713	In vitro antibacterial results showed that the bacteria numbers decreased with an increase in the loading amount of ε-polylysine.
32204005	8	62	theme	implant	1499:1505	arg1	property					1545:1552	improved antibacterial property	1522:1552	improved antibacterial property	1522:1552	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	8	62	theme	implant	1499:1505	arg1	biocompatibility					1568:1583	excellent biocompatibility	1558:1583	excellent biocompatibility	1558:1583	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	8	62	theme	implant	1499:1505	arg1	materials					1507:1515	the orthopedic implant materials	1484:1515	the orthopedic implant materials both improved antibacterial property and excellent biocompatibility	1484:1583	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	8	63	theme	implants	1664:1671	arg1	application					1634:1644	practical application	1624:1644	practical application of titanium-based implants	1624:1671	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	4	64	theme	composite	553:561	arg1	films					563:567	ε-polylysine and gum Arabic multilayer composite films	514:567	ε-polylysine and gum Arabic multilayer composite films	514:567	In this work, ε-polylysine and gum Arabic multilayer composite films were immobilized layer by layer (LBL) on anodized titanium with the assistance of polydopamine for the first time.
32204005	8	65	theme	orthopedic	1488:1497	arg1	property					1545:1552	improved antibacterial property	1522:1552	improved antibacterial property	1522:1552	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	8	65	theme	orthopedic	1488:1497	arg1	biocompatibility					1568:1583	excellent biocompatibility	1558:1583	excellent biocompatibility	1558:1583	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	8	65	theme	orthopedic	1488:1497	arg1	materials					1507:1515	the orthopedic implant materials	1484:1515	the orthopedic implant materials both improved antibacterial property and excellent biocompatibility	1484:1583	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	1	66	from	failure	214:220	arg1	applications					254:265	clinical applications	245:265	clinical applications	245:265	Bacterial infection has become a crucial reason that give rise to failure of medical implants in clinical applications.
32204005	1	67	theme	Bacterial	148:156	arg1	infection					158:166	Bacterial infection	148:166	Bacterial infection	148:166	Bacterial infection has become a crucial reason that give rise to failure of medical implants in clinical applications.
32204005	8	68	theme	Arabic	1399:1404	arg1	films					1427:1431	the ε-polylysine and gum Arabic multilayer composite films	1374:1431	the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine	1374:1463	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	8	68	theme	Arabic	1399:1404	arg1	able					1470:1473	able	1470:1473	able	1470:1473	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	1	69	theme	implants	233:240	arg1	failure					214:220	failure	214:220	failure of medical implants in clinical applications	214:265	Bacterial infection has become a crucial reason that give rise to failure of medical implants in clinical applications.
32204005	5	70	theme	antibacterial	693:705	arg1	results					707:713	In vitro antibacterial results	684:713	In vitro antibacterial results	684:713	In vitro antibacterial results showed that the bacteria numbers decreased with an increase in the loading amount of ε-polylysine.
32204005	7	71	dep	proliferation	1075:1087	arg1	the					1066:1068	the	1066:1068	the	1066:1068	Meanwhile, the cell proliferation and osteogenic differentiation of BMSCs on titanium dioxide nanotubes (TNTs) modified with composite films was significantly improved.
32204005	6	72	theme	binding	1030:1036	arg1	merits					1011:1016	the merits	1007:1016	the merits of covalent binding and LBL methods	1007:1052	Furthermore, long-term antibacterial property up to 3 weeks against both gram-positive Staphylococcus aureus (S. aureus) and gram-negative Escherichia coli (E. coli) was obtained combined with the merits of covalent binding and LBL methods.
32204005	0	73	theme	titanium	84:91	arg1	Tailoring					94:102	titanium: Tailoring	84:102	titanium: Tailoring of antibacterial and osteogenic properties	84:145	Layer-by-layer immobilizing of polydopamine-assisted ε-polylysine and gum Arabic on titanium: Tailoring of antibacterial and osteogenic properties.
32204005	5	74	from	increase	766:773	arg1	ε-polylysine					800:811	ε-polylysine	800:811	ε-polylysine	800:811	In vitro antibacterial results showed that the bacteria numbers decreased with an increase in the loading amount of ε-polylysine.
32204005	5	74	from	increase	766:773	arg1	amount					790:795	the loading amount	778:795	the loading amount of ε-polylysine	778:811	In vitro antibacterial results showed that the bacteria numbers decreased with an increase in the loading amount of ε-polylysine.
32204005	8	75	theme	osteogenic	1283:1292	arg1	properties					1294:1303	anti-infective and osteogenic properties	1264:1303	anti-infective and osteogenic properties of medical titanium	1264:1323	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	1	76	theme	clinical	245:252	arg1	applications					254:265	clinical applications	245:265	clinical applications	245:265	Bacterial infection has become a crucial reason that give rise to failure of medical implants in clinical applications.
32204005	8	77	theme	facile	1238:1243	arg1	method					1245:1250	a facile method	1236:1250	a facile method to optimize anti-infective and osteogenic properties of medical titanium	1236:1323	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	4	78	theme	ε-polylysine	514:525	arg1	films					563:567	ε-polylysine and gum Arabic multilayer composite films	514:567	ε-polylysine and gum Arabic multilayer composite films	514:567	In this work, ε-polylysine and gum Arabic multilayer composite films were immobilized layer by layer (LBL) on anodized titanium with the assistance of polydopamine for the first time.
32204005	8	79	theme	polydopamine	1452:1463	arg1	films					1427:1431	the ε-polylysine and gum Arabic multilayer composite films	1374:1431	the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine	1374:1463	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	8	79	theme	polydopamine	1452:1463	arg1	able					1470:1473	able	1470:1473	able	1470:1473	Remarkably, a facile method to optimize anti-infective and osteogenic properties of medical titanium has been developed, and it was demonstrated that the ε-polylysine and gum Arabic multilayer composite films with assistance of polydopamine were able to endow the orthopedic implant materials both improved antibacterial property and excellent biocompatibility, which is of profound significance for practical application of titanium-based implants.
32204005	4	80	theme	anodized	610:617	arg1	titanium					619:626	anodized titanium	610:626	anodized titanium with the assistance of polydopamine for the first time	610:681	In this work, ε-polylysine and gum Arabic multilayer composite films were immobilized layer by layer (LBL) on anodized titanium with the assistance of polydopamine for the first time.
32204005	6	81	dep	3 weeks	866:872	arg1	up					860:861	up	860:861	up	860:861	Furthermore, long-term antibacterial property up to 3 weeks against both gram-positive Staphylococcus aureus (S. aureus) and gram-negative Escherichia coli (E. coli) was obtained combined with the merits of covalent binding and LBL methods.
32721833	6	0	theme	complete	903:910	arg1	process					927:933	complete gelatinization process	903:933	complete gelatinization process	903:933	Extrusion also increased starch digestibility due to complete gelatinization process, making it almost equal for all flours.
32721833	3	1	theme	raw	485:487	arg1	material					489:496	an adequate raw material	473:496	an adequate raw material for the bakery and confectionery industry	473:538	Modification of physico-chemical properties of non-wheat flours by dry heat and extrusion represent the alternative process which can transform the flours into an adequate raw material for the bakery and confectionery industry.
32721833	4	2	theme	study	557:561	arg1	aim					545:547	The aim	541:547	The aim of this study	541:561	The aim of this study was to determine the type and extent of physico-chemical changes in modified flours whose mixtures were used successfully for bread production.
32721833	3	3	theme	physico-chemical	329:344	arg1	properties					346:355	physico-chemical properties	329:355	physico-chemical properties of non-wheat flours	329:375	Modification of physico-chemical properties of non-wheat flours by dry heat and extrusion represent the alternative process which can transform the flours into an adequate raw material for the bakery and confectionery industry.
32721833	3	4	theme	confectionery	517:529	arg1	industry					531:538	the bakery and confectionery industry	502:538	industry	531:538	Modification of physico-chemical properties of non-wheat flours by dry heat and extrusion represent the alternative process which can transform the flours into an adequate raw material for the bakery and confectionery industry.
32721833	3	5	theme	properties	346:355	arg1	Modification					313:324	Modification	313:324	Modification of physico-chemical properties of non-wheat flours by dry heat and extrusion	313:401	Modification of physico-chemical properties of non-wheat flours by dry heat and extrusion represent the alternative process which can transform the flours into an adequate raw material for the bakery and confectionery industry.
32721833	5	6	theme	phenolic	830:837	arg1	compounds					839:847	phenolic compounds	830:847	phenolic compounds	830:847	Extrusion had stronger influence on chemical composition of flours than dry heating, especially on the content of fats and phenolic compounds.
32721833	5	7	theme	stronger	721:728	arg1	influence					730:738	stronger influence	721:738	stronger influence on chemical composition of flours	721:772	Extrusion had stronger influence on chemical composition of flours than dry heating, especially on the content of fats and phenolic compounds.
32721833	4	8	theme	bread	689:693	arg1	production					695:704	bread production	689:704	bread production	689:704	The aim of this study was to determine the type and extent of physico-chemical changes in modified flours whose mixtures were used successfully for bread production.
32721833	3	9	theme	bakery	506:511	arg1	industry					531:538	the bakery and confectionery industry	502:538	industry	531:538	Modification of physico-chemical properties of non-wheat flours by dry heat and extrusion represent the alternative process which can transform the flours into an adequate raw material for the bakery and confectionery industry.
32721833	4	10	from	flours	640:645	arg1	extent					593:598	extent	593:598	extent	593:598	The aim of this study was to determine the type and extent of physico-chemical changes in modified flours whose mixtures were used successfully for bread production.
32721833	4	10	from	flours	640:645	arg1	type					584:587	type	584:587	type	584:587	The aim of this study was to determine the type and extent of physico-chemical changes in modified flours whose mixtures were used successfully for bread production.
32721833	3	11	theme	alternative	417:427	arg1	process					429:435	the alternative process	413:435	the alternative process which can transform the flours into an adequate raw material for the bakery and confectionery industry	413:538	Modification of physico-chemical properties of non-wheat flours by dry heat and extrusion represent the alternative process which can transform the flours into an adequate raw material for the bakery and confectionery industry.
32721833	5	12	contain	had	717:719	arg1	Extrusion					707:715	Extrusion	707:715	Extrusion	707:715	Extrusion had stronger influence on chemical composition of flours than dry heating, especially on the content of fats and phenolic compounds.
32721833	5	12	contain	had	717:719	arg2	influence					730:738	stronger influence	721:738	stronger influence on chemical composition of flours	721:772	Extrusion had stronger influence on chemical composition of flours than dry heating, especially on the content of fats and phenolic compounds.
32721833	5	13	theme	flours	767:772	arg1	composition					752:762	chemical composition	743:762	chemical composition of flours	743:772	Extrusion had stronger influence on chemical composition of flours than dry heating, especially on the content of fats and phenolic compounds.
32721833	4	14	used	used	667:670	arg2	mixtures					653:660	mixtures	653:660	mixtures	653:660	The aim of this study was to determine the type and extent of physico-chemical changes in modified flours whose mixtures were used successfully for bread production.
32721833	5	15	theme	compounds	839:847	arg1	content					810:816	the content	806:816	the content of fats and phenolic compounds	806:847	Extrusion had stronger influence on chemical composition of flours than dry heating, especially on the content of fats and phenolic compounds.
32721833	3	16	theme	flours	370:375	arg1	properties					346:355	physico-chemical properties	329:355	physico-chemical properties of non-wheat flours	329:375	Modification of physico-chemical properties of non-wheat flours by dry heat and extrusion represent the alternative process which can transform the flours into an adequate raw material for the bakery and confectionery industry.
32721833	6	17	theme	starch	875:880	arg1	digestibility					882:894	starch digestibility	875:894	starch digestibility	875:894	Extrusion also increased starch digestibility due to complete gelatinization process, making it almost equal for all flours.
32721833	4	18	from	changes	620:626	arg1	flours					640:645	modified flours	631:645	modified flours whose mixtures were used successfully for bread production	631:704	The aim of this study was to determine the type and extent of physico-chemical changes in modified flours whose mixtures were used successfully for bread production.
32721833	5	19	from	influence	730:738	arg1	composition					752:762	chemical composition	743:762	chemical composition of flours	743:772	Extrusion had stronger influence on chemical composition of flours than dry heating, especially on the content of fats and phenolic compounds.
32721833	5	20	theme	dry	779:781	arg1	heating					783:789	dry heating	779:789	dry heating	779:789	Extrusion had stronger influence on chemical composition of flours than dry heating, especially on the content of fats and phenolic compounds.
32721833	3	21	theme	non-wheat	360:368	arg1	flours					370:375	non-wheat flours	360:375	non-wheat flours	360:375	Modification of physico-chemical properties of non-wheat flours by dry heat and extrusion represent the alternative process which can transform the flours into an adequate raw material for the bakery and confectionery industry.
32721833	7	22	theme	flour	1054:1058	arg1	origin					1044:1049	botanical origin	1034:1049	botanical origin of flour	1034:1058	On the other hand, protein digestibility depends mostly on botanical origin of flour.
32721833	2	23	theme	final	267:271	arg1	products					273:280	the final products	263:280	the final products	263:280	Some of these flours can be conventionally processed, but the final products are not of acceptable quality.
32721833	0	24	theme	non-wheat	36:44	arg1	flours					46:51	non-wheat flours	36:51	non-wheat flours	36:51	Heat and hydrothermal treatments of non-wheat flours.
32721833	7	25	theme	botanical	1034:1042	arg1	origin					1044:1049	botanical origin	1034:1049	botanical origin of flour	1034:1058	On the other hand, protein digestibility depends mostly on botanical origin of flour.
32721833	5	26	theme	fats	821:824	arg1	content					810:816	the content	806:816	the content of fats and phenolic compounds	806:847	Extrusion had stronger influence on chemical composition of flours than dry heating, especially on the content of fats and phenolic compounds.
32721833	4	27	from	extent	593:598	arg1	flours					640:645	modified flours	631:645	modified flours whose mixtures were used successfully for bread production	631:704	The aim of this study was to determine the type and extent of physico-chemical changes in modified flours whose mixtures were used successfully for bread production.
32721833	4	28	theme	changes	620:626	arg1	extent					593:598	extent	593:598	extent	593:598	The aim of this study was to determine the type and extent of physico-chemical changes in modified flours whose mixtures were used successfully for bread production.
32721833	4	28	theme	changes	620:626	arg1	type					584:587	type	584:587	type	584:587	The aim of this study was to determine the type and extent of physico-chemical changes in modified flours whose mixtures were used successfully for bread production.
32721833	4	29	theme	modified	631:638	arg1	flours					640:645	modified flours	631:645	modified flours whose mixtures were used successfully for bread production	631:704	The aim of this study was to determine the type and extent of physico-chemical changes in modified flours whose mixtures were used successfully for bread production.
32721833	1	30	theme	proteins	195:202	arg1	properties					175:184	properties	175:184	properties of their proteins	175:202	Non-wheat cereals have become popular in the diet due to their nutritional benefits, but their application is limited by properties of their proteins.
32721833	3	31	theme	dry	380:382	arg1	heat					384:387	dry heat	380:387	dry heat	380:387	Modification of physico-chemical properties of non-wheat flours by dry heat and extrusion represent the alternative process which can transform the flours into an adequate raw material for the bakery and confectionery industry.
32721833	5	32	theme	chemical	743:750	arg1	composition					752:762	chemical composition	743:762	chemical composition of flours	743:772	Extrusion had stronger influence on chemical composition of flours than dry heating, especially on the content of fats and phenolic compounds.
32721833	7	33	theme	protein	994:1000	arg1	digestibility					1002:1014	protein digestibility	994:1014	protein digestibility	994:1014	On the other hand, protein digestibility depends mostly on botanical origin of flour.
32721833	1	34	theme	due	104:106	arg1	diet					99:102	the diet	95:102	the diet due to their nutritional benefits	95:136	Non-wheat cereals have become popular in the diet due to their nutritional benefits, but their application is limited by properties of their proteins.
32721833	0	35	theme	flours	46:51	arg1	Heat					0:3	Heat	0:3	Heat	0:3	Heat and hydrothermal treatments of non-wheat flours.
32721833	0	35	theme	flours	46:51	arg1	treatments					22:31	hydrothermal treatments	9:31	hydrothermal treatments	9:31	Heat and hydrothermal treatments of non-wheat flours.
32721833	7	36	theme	other	982:986	arg1	hand					988:991	the other hand	978:991	the other hand	978:991	On the other hand, protein digestibility depends mostly on botanical origin of flour.
32721833	3	37	theme	adequate	476:483	arg1	material					489:496	an adequate raw material	473:496	an adequate raw material for the bakery and confectionery industry	473:538	Modification of physico-chemical properties of non-wheat flours by dry heat and extrusion represent the alternative process which can transform the flours into an adequate raw material for the bakery and confectionery industry.
32721833	4	38	dep	type	584:587	arg1	the					580:582	the	580:582	the	580:582	The aim of this study was to determine the type and extent of physico-chemical changes in modified flours whose mixtures were used successfully for bread production.
32721833	2	39	theme	acceptable	293:302	arg1	quality					304:310	acceptable quality	293:310	acceptable quality	293:310	Some of these flours can be conventionally processed, but the final products are not of acceptable quality.
32721833	1	40	from	diet	99:102	arg1	popular					84:90	popular	84:90	popular	84:90	Non-wheat cereals have become popular in the diet due to their nutritional benefits, but their application is limited by properties of their proteins.
32721833	1	41	theme	Non-wheat	54:62	arg1	cereals					64:70	Non-wheat cereals	54:70	Non-wheat cereals	54:70	Non-wheat cereals have become popular in the diet due to their nutritional benefits, but their application is limited by properties of their proteins.
32721833	1	42	theme	nutritional	117:127	arg1	benefits					129:136	their nutritional benefits	111:136	their nutritional benefits	111:136	Non-wheat cereals have become popular in the diet due to their nutritional benefits, but their application is limited by properties of their proteins.
32721833	1	43	from	popular	84:90	arg1	diet					99:102	the diet	95:102	the diet due to their nutritional benefits	95:136	Non-wheat cereals have become popular in the diet due to their nutritional benefits, but their application is limited by properties of their proteins.
32721833	4	44	theme	physico-chemical	603:618	arg1	changes					620:626	physico-chemical changes	603:626	physico-chemical changes in modified flours whose mixtures were used successfully for bread production	603:704	The aim of this study was to determine the type and extent of physico-chemical changes in modified flours whose mixtures were used successfully for bread production.
32721833	4	45	from	type	584:587	arg1	flours					640:645	modified flours	631:645	modified flours whose mixtures were used successfully for bread production	631:704	The aim of this study was to determine the type and extent of physico-chemical changes in modified flours whose mixtures were used successfully for bread production.
32721833	6	46	theme	gelatinization	912:925	arg1	process					927:933	complete gelatinization process	903:933	complete gelatinization process	903:933	Extrusion also increased starch digestibility due to complete gelatinization process, making it almost equal for all flours.
32721833	0	47	theme	hydrothermal	9:20	arg1	treatments					22:31	hydrothermal treatments	9:31	hydrothermal treatments	9:31	Heat and hydrothermal treatments of non-wheat flours.
32394135	8	0	theme	synthetic	1102:1110	arg1	nanosheets					1140:1149	few-layered MoS2 nanosheets	1123:1149	few-layered MoS2 nanosheets	1123:1149	The good response is attributed to the synergistic enhancement of the synthetic nanozymes (few-layered MoS2 nanosheets) and immobilized natural horseradish peroxidase (HRP).
32394135	8	0	theme	synthetic	1102:1110	arg1	nanozymes					1112:1120	the synthetic nanozymes	1098:1120	the synthetic nanozymes (few-layered MoS2 nanosheets)	1098:1150	The good response is attributed to the synergistic enhancement of the synthetic nanozymes (few-layered MoS2 nanosheets) and immobilized natural horseradish peroxidase (HRP).
32394135	6	1	theme	electrochemical	728:742	arg1	activity					744:751	high electrochemical activity	723:751	high electrochemical activity of immobilized enzyme	723:773	The use of MoS2 nanosheets warrants high electrochemical activity of immobilized enzyme on the electrode surface.
32394135	8	2	theme	nanozymes	1112:1120	arg1	enhancement					1083:1093	the synergistic enhancement	1067:1093	the synergistic enhancement of the synthetic nanozymes (few-layered MoS2 nanosheets) and immobilized natural horseradish peroxidase (HRP)	1067:1203	The good response is attributed to the synergistic enhancement of the synthetic nanozymes (few-layered MoS2 nanosheets) and immobilized natural horseradish peroxidase (HRP).
32394135	1	3	theme	nanosheets	243:252	arg1	use					231:233	the use	227:233	the use of MoS2 nanosheets and graphite that are assembled to form a microfiber hybrid structure	227:322	A biosensor for hydrogen peroxide (H2O2) has been developed based on the use of MoS2 nanosheets and graphite that are assembled to form a microfiber hybrid structure.
32394135	7	4	theme	modified	805:812	arg1	electrode					825:833	The modified microfiber electrode	801:833	The modified microfiber electrode	801:833	The modified microfiber electrode, best operated at a voltage of - 0.3 V (vs. Ag/AgCl), can be used to sense H2O2 with a linear response in the 0.1 to 90 μM concentration range and with a determination limit of 30 nM (at S/N = 3).
32394135	1	5	theme	hydrogen	174:181	arg1	H2O2					193:196	H2O2	193:196	H2O2	193:196	A biosensor for hydrogen peroxide (H2O2) has been developed based on the use of MoS2 nanosheets and graphite that are assembled to form a microfiber hybrid structure.
32394135	1	5	theme	hydrogen	174:181	arg1	peroxide					183:190	hydrogen peroxide	174:190	hydrogen peroxide (H2O2)	174:197	A biosensor for hydrogen peroxide (H2O2) has been developed based on the use of MoS2 nanosheets and graphite that are assembled to form a microfiber hybrid structure.
32394135	7	6	from	S/N = 3	1022:1028	arg1	30 nM					1012:1016	30 nM	1012:1016	30 nM (at S/N = 3)	1012:1029	The modified microfiber electrode, best operated at a voltage of - 0.3 V (vs. Ag/AgCl), can be used to sense H2O2 with a linear response in the 0.1 to 90 μM concentration range and with a determination limit of 30 nM (at S/N = 3).
32394135	7	6	from	S/N = 3	1022:1028	arg1	limit					1003:1007	a determination limit	987:1007	a determination limit of 30 nM (at S/N = 3)	987:1029	The modified microfiber electrode, best operated at a voltage of - 0.3 V (vs. Ag/AgCl), can be used to sense H2O2 with a linear response in the 0.1 to 90 μM concentration range and with a determination limit of 30 nM (at S/N = 3).
32394135	0	7	theme	enhanced	100:107	arg1	determination					122:134	enhanced voltammetric determination	100:134	enhanced voltammetric determination of hydrogen peroxide	100:155	Synergy between nanozymes and natural enzymes on the hybrid MoS2 nanosheets/graphite microfiber for enhanced voltammetric determination of hydrogen peroxide.
32394135	0	8	from	Synergy	0:6	arg1	MoS2					60:63	the hybrid MoS2	49:63	the hybrid MoS2	49:63	Synergy between nanozymes and natural enzymes on the hybrid MoS2 nanosheets/graphite microfiber for enhanced voltammetric determination of hydrogen peroxide.
32394135	5	9	theme	microfiber	665:674	arg1	electrode					676:684	the microfiber electrode	661:684	the microfiber electrode	661:684	In the next step, horseradish peroxidase, methylene blue, and chitosan are co-immobilized on the microfiber electrode.
32394135	7	10	theme	90 μM	952:956	arg1	range					972:976	the 0.1 to 90 μM concentration range	941:976	the 0.1 to 90 μM concentration range	941:976	The modified microfiber electrode, best operated at a voltage of - 0.3 V (vs. Ag/AgCl), can be used to sense H2O2 with a linear response in the 0.1 to 90 μM concentration range and with a determination limit of 30 nM (at S/N = 3).
32394135	1	11	theme	graphite	258:265	arg1	use					231:233	the use	227:233	the use of MoS2 nanosheets and graphite that are assembled to form a microfiber hybrid structure	227:322	A biosensor for hydrogen peroxide (H2O2) has been developed based on the use of MoS2 nanosheets and graphite that are assembled to form a microfiber hybrid structure.
32394135	7	12	theme	concentration	958:970	arg1	range					972:976	the 0.1 to 90 μM concentration range	941:976	the 0.1 to 90 μM concentration range	941:976	The modified microfiber electrode, best operated at a voltage of - 0.3 V (vs. Ag/AgCl), can be used to sense H2O2 with a linear response in the 0.1 to 90 μM concentration range and with a determination limit of 30 nM (at S/N = 3).
32394135	5	13	theme	blue	620:623	arg1	methylene					610:618	methylene blue	610:623	methylene blue	610:623	In the next step, horseradish peroxidase, methylene blue, and chitosan are co-immobilized on the microfiber electrode.
32394135	6	14	theme	high	723:726	arg1	activity					744:751	high electrochemical activity	723:751	high electrochemical activity of immobilized enzyme	723:773	The use of MoS2 nanosheets warrants high electrochemical activity of immobilized enzyme on the electrode surface.
32394135	7	15	theme	- 0.3 V	866:872	arg1	voltage					855:861	a voltage	853:861	a voltage of - 0.3 V (vs. Ag/AgCl)	853:886	The modified microfiber electrode, best operated at a voltage of - 0.3 V (vs. Ag/AgCl), can be used to sense H2O2 with a linear response in the 0.1 to 90 μM concentration range and with a determination limit of 30 nM (at S/N = 3).
32394135	8	16	theme	MoS2	1135:1138	arg1	nanosheets					1140:1149	few-layered MoS2 nanosheets	1123:1149	few-layered MoS2 nanosheets	1123:1149	The good response is attributed to the synergistic enhancement of the synthetic nanozymes (few-layered MoS2 nanosheets) and immobilized natural horseradish peroxidase (HRP).
32394135	8	16	theme	MoS2	1135:1138	arg1	nanozymes					1112:1120	the synthetic nanozymes	1098:1120	the synthetic nanozymes (few-layered MoS2 nanosheets)	1098:1150	The good response is attributed to the synergistic enhancement of the synthetic nanozymes (few-layered MoS2 nanosheets) and immobilized natural horseradish peroxidase (HRP).
32394135	0	17	theme	voltammetric	109:120	arg1	determination					122:134	enhanced voltammetric determination	100:134	enhanced voltammetric determination of hydrogen peroxide	100:155	Synergy between nanozymes and natural enzymes on the hybrid MoS2 nanosheets/graphite microfiber for enhanced voltammetric determination of hydrogen peroxide.
32394135	8	18	theme	horseradish	1176:1186	arg1	HRP					1200:1202	HRP	1200:1202	HRP	1200:1202	The good response is attributed to the synergistic enhancement of the synthetic nanozymes (few-layered MoS2 nanosheets) and immobilized natural horseradish peroxidase (HRP).
32394135	8	18	theme	horseradish	1176:1186	arg1	peroxidase					1188:1197	immobilized natural horseradish peroxidase	1156:1197	immobilized natural horseradish peroxidase (HRP)	1156:1203	The good response is attributed to the synergistic enhancement of the synthetic nanozymes (few-layered MoS2 nanosheets) and immobilized natural horseradish peroxidase (HRP).
32394135	9	19	dep	abstract	1215:1222	arg1	Grapical					1206:1213	Grapical	1206:1213	Grapical	1206:1213	Grapical abstract.
32394135	6	20	theme	nanosheets	703:712	arg1	use					691:693	The use	687:693	The use of MoS2 nanosheets	687:712	The use of MoS2 nanosheets warrants high electrochemical activity of immobilized enzyme on the electrode surface.
32394135	2	21	theme	graphite	374:381	arg1	microfiber					383:392	a graphite microfiber	372:392	a graphite microfiber	372:392	The MoS2 nanosheets are synthesized in situ on a graphite microfiber.
32394135	7	22	theme	linear	922:927	arg1	response					929:936	a linear response	920:936	a linear response in the 0.1 to 90 μM concentration range	920:976	The modified microfiber electrode, best operated at a voltage of - 0.3 V (vs. Ag/AgCl), can be used to sense H2O2 with a linear response in the 0.1 to 90 μM concentration range and with a determination limit of 30 nM (at S/N = 3).
32394135	6	23	theme	MoS2	698:701	arg1	nanosheets					703:712	MoS2 nanosheets	698:712	MoS2 nanosheets	698:712	The use of MoS2 nanosheets warrants high electrochemical activity of immobilized enzyme on the electrode surface.
32394135	3	24	dep	composition	408:418	arg1	The					395:397	The	395:397	The	395:397	The chemical composition and surface morphology of the microfiber hybrid structure has been characterized.
32394135	5	25	theme	horseradish	586:596	arg1	peroxidase					598:607	horseradish peroxidase	586:607	horseradish peroxidase	586:607	In the next step, horseradish peroxidase, methylene blue, and chitosan are co-immobilized on the microfiber electrode.
32394135	3	26	theme	surface	424:430	arg1	morphology					432:441	surface morphology	424:441	surface morphology	424:441	The chemical composition and surface morphology of the microfiber hybrid structure has been characterized.
32394135	8	27	theme	immobilized	1156:1166	arg1	HRP					1200:1202	HRP	1200:1202	HRP	1200:1202	The good response is attributed to the synergistic enhancement of the synthetic nanozymes (few-layered MoS2 nanosheets) and immobilized natural horseradish peroxidase (HRP).
32394135	8	27	theme	immobilized	1156:1166	arg1	peroxidase					1188:1197	immobilized natural horseradish peroxidase	1156:1197	immobilized natural horseradish peroxidase (HRP)	1156:1203	The good response is attributed to the synergistic enhancement of the synthetic nanozymes (few-layered MoS2 nanosheets) and immobilized natural horseradish peroxidase (HRP).
32394135	8	28	theme	few-layered	1123:1133	arg1	nanosheets					1140:1149	few-layered MoS2 nanosheets	1123:1149	few-layered MoS2 nanosheets	1123:1149	The good response is attributed to the synergistic enhancement of the synthetic nanozymes (few-layered MoS2 nanosheets) and immobilized natural horseradish peroxidase (HRP).
32394135	8	28	theme	few-layered	1123:1133	arg1	nanozymes					1112:1120	the synthetic nanozymes	1098:1120	the synthetic nanozymes (few-layered MoS2 nanosheets)	1098:1150	The good response is attributed to the synergistic enhancement of the synthetic nanozymes (few-layered MoS2 nanosheets) and immobilized natural horseradish peroxidase (HRP).
32394135	0	29	theme	hydrogen	139:146	arg1	peroxide					148:155	hydrogen peroxide	139:155	hydrogen peroxide	139:155	Synergy between nanozymes and natural enzymes on the hybrid MoS2 nanosheets/graphite microfiber for enhanced voltammetric determination of hydrogen peroxide.
32394135	7	30	dep	90 μM	952:956	arg1	to					949:950	to	949:950	to	949:950	The modified microfiber electrode, best operated at a voltage of - 0.3 V (vs. Ag/AgCl), can be used to sense H2O2 with a linear response in the 0.1 to 90 μM concentration range and with a determination limit of 30 nM (at S/N = 3).
32394135	3	31	theme	hybrid	461:466	arg1	structure					468:476	the microfiber hybrid structure	446:476	the microfiber hybrid structure	446:476	The chemical composition and surface morphology of the microfiber hybrid structure has been characterized.
32394135	7	32	theme	microfiber	814:823	arg1	electrode					825:833	The modified microfiber electrode	801:833	The modified microfiber electrode	801:833	The modified microfiber electrode, best operated at a voltage of - 0.3 V (vs. Ag/AgCl), can be used to sense H2O2 with a linear response in the 0.1 to 90 μM concentration range and with a determination limit of 30 nM (at S/N = 3).
32394135	3	33	theme	structure	468:476	arg1	morphology					432:441	surface morphology	424:441	surface morphology	424:441	The chemical composition and surface morphology of the microfiber hybrid structure has been characterized.
32394135	3	33	theme	structure	468:476	arg1	composition					408:418	chemical composition	399:418	chemical composition	399:418	The chemical composition and surface morphology of the microfiber hybrid structure has been characterized.
32394135	3	34	theme	chemical	399:406	arg1	composition					408:418	chemical composition	399:418	chemical composition	399:418	The chemical composition and surface morphology of the microfiber hybrid structure has been characterized.
32394135	0	35	theme	natural	30:36	arg1	enzymes					38:44	natural enzymes	30:44	natural enzymes	30:44	Synergy between nanozymes and natural enzymes on the hybrid MoS2 nanosheets/graphite microfiber for enhanced voltammetric determination of hydrogen peroxide.
32394135	0	36	theme	peroxide	148:155	arg1	determination					122:134	enhanced voltammetric determination	100:134	enhanced voltammetric determination of hydrogen peroxide	100:155	Synergy between nanozymes and natural enzymes on the hybrid MoS2 nanosheets/graphite microfiber for enhanced voltammetric determination of hydrogen peroxide.
32394135	6	37	theme	electrode	782:790	arg1	surface					792:798	the electrode surface	778:798	the electrode surface	778:798	The use of MoS2 nanosheets warrants high electrochemical activity of immobilized enzyme on the electrode surface.
32394135	3	38	theme	microfiber	450:459	arg1	structure					468:476	the microfiber hybrid structure	446:476	the microfiber hybrid structure	446:476	The chemical composition and surface morphology of the microfiber hybrid structure has been characterized.
32394135	1	39	theme	microfiber	296:305	arg1	structure					314:322	a microfiber hybrid structure	294:322	a microfiber hybrid structure	294:322	A biosensor for hydrogen peroxide (H2O2) has been developed based on the use of MoS2 nanosheets and graphite that are assembled to form a microfiber hybrid structure.
32394135	7	40	theme	30 nM	1012:1016	arg1	limit					1003:1007	a determination limit	987:1007	a determination limit of 30 nM (at S/N = 3)	987:1029	The modified microfiber electrode, best operated at a voltage of - 0.3 V (vs. Ag/AgCl), can be used to sense H2O2 with a linear response in the 0.1 to 90 μM concentration range and with a determination limit of 30 nM (at S/N = 3).
32394135	7	41	theme	determination	989:1001	arg1	limit					1003:1007	a determination limit	987:1007	a determination limit of 30 nM (at S/N = 3)	987:1029	The modified microfiber electrode, best operated at a voltage of - 0.3 V (vs. Ag/AgCl), can be used to sense H2O2 with a linear response in the 0.1 to 90 μM concentration range and with a determination limit of 30 nM (at S/N = 3).
32394135	8	42	theme	peroxidase	1188:1197	arg1	enhancement					1083:1093	the synergistic enhancement	1067:1093	the synergistic enhancement of the synthetic nanozymes (few-layered MoS2 nanosheets) and immobilized natural horseradish peroxidase (HRP)	1067:1203	The good response is attributed to the synergistic enhancement of the synthetic nanozymes (few-layered MoS2 nanosheets) and immobilized natural horseradish peroxidase (HRP).
32394135	1	43	theme	hybrid	307:312	arg1	structure					314:322	a microfiber hybrid structure	294:322	a microfiber hybrid structure	294:322	A biosensor for hydrogen peroxide (H2O2) has been developed based on the use of MoS2 nanosheets and graphite that are assembled to form a microfiber hybrid structure.
32394135	8	44	theme	good	1036:1039	arg1	response					1041:1048	The good response	1032:1048	The good response	1032:1048	The good response is attributed to the synergistic enhancement of the synthetic nanozymes (few-layered MoS2 nanosheets) and immobilized natural horseradish peroxidase (HRP).
32394135	2	45	theme	MoS2	329:332	arg1	nanosheets					334:343	The MoS2 nanosheets	325:343	The MoS2 nanosheets	325:343	The MoS2 nanosheets are synthesized in situ on a graphite microfiber.
32394135	4	46	theme	peroxidase-mimicking	537:556	arg1	activity					558:565	peroxidase-mimicking activity	537:565	peroxidase-mimicking activity	537:565	The microfiber is shown to display peroxidase-mimicking activity.
32394135	8	47	theme	synergistic	1071:1081	arg1	enhancement					1083:1093	the synergistic enhancement	1067:1093	the synergistic enhancement of the synthetic nanozymes (few-layered MoS2 nanosheets) and immobilized natural horseradish peroxidase (HRP)	1067:1203	The good response is attributed to the synergistic enhancement of the synthetic nanozymes (few-layered MoS2 nanosheets) and immobilized natural horseradish peroxidase (HRP).
32394135	5	48	theme	next	575:578	arg1	step					580:583	the next step	571:583	the next step	571:583	In the next step, horseradish peroxidase, methylene blue, and chitosan are co-immobilized on the microfiber electrode.
32394135	7	49	theme	sense	904:908	arg1	H2O2					910:913	sense H2O2	904:913	sense H2O2	904:913	The modified microfiber electrode, best operated at a voltage of - 0.3 V (vs. Ag/AgCl), can be used to sense H2O2 with a linear response in the 0.1 to 90 μM concentration range and with a determination limit of 30 nM (at S/N = 3).
32394135	7	50	from	limit	1003:1007	arg1	S/N = 3					1022:1028	S/N = 3	1022:1028	S/N = 3	1022:1028	The modified microfiber electrode, best operated at a voltage of - 0.3 V (vs. Ag/AgCl), can be used to sense H2O2 with a linear response in the 0.1 to 90 μM concentration range and with a determination limit of 30 nM (at S/N = 3).
32394135	6	51	theme	enzyme	768:773	arg1	activity					744:751	high electrochemical activity	723:751	high electrochemical activity of immobilized enzyme	723:773	The use of MoS2 nanosheets warrants high electrochemical activity of immobilized enzyme on the electrode surface.
32394135	7	52	used	used	896:899	arg2	electrode					825:833	The modified microfiber electrode	801:833	The modified microfiber electrode	801:833	The modified microfiber electrode, best operated at a voltage of - 0.3 V (vs. Ag/AgCl), can be used to sense H2O2 with a linear response in the 0.1 to 90 μM concentration range and with a determination limit of 30 nM (at S/N = 3).
32394135	6	53	theme	immobilized	756:766	arg1	enzyme					768:773	immobilized enzyme	756:773	immobilized enzyme	756:773	The use of MoS2 nanosheets warrants high electrochemical activity of immobilized enzyme on the electrode surface.
32394135	8	54	theme	natural	1168:1174	arg1	HRP					1200:1202	HRP	1200:1202	HRP	1200:1202	The good response is attributed to the synergistic enhancement of the synthetic nanozymes (few-layered MoS2 nanosheets) and immobilized natural horseradish peroxidase (HRP).
32394135	8	54	theme	natural	1168:1174	arg1	peroxidase					1188:1197	immobilized natural horseradish peroxidase	1156:1197	immobilized natural horseradish peroxidase (HRP)	1156:1203	The good response is attributed to the synergistic enhancement of the synthetic nanozymes (few-layered MoS2 nanosheets) and immobilized natural horseradish peroxidase (HRP).
32394135	7	55	from	response	929:936	arg1	range					972:976	the 0.1 to 90 μM concentration range	941:976	the 0.1 to 90 μM concentration range	941:976	The modified microfiber electrode, best operated at a voltage of - 0.3 V (vs. Ag/AgCl), can be used to sense H2O2 with a linear response in the 0.1 to 90 μM concentration range and with a determination limit of 30 nM (at S/N = 3).
32394135	0	56	theme	hybrid	53:58	arg1	MoS2					60:63	the hybrid MoS2	49:63	the hybrid MoS2	49:63	Synergy between nanozymes and natural enzymes on the hybrid MoS2 nanosheets/graphite microfiber for enhanced voltammetric determination of hydrogen peroxide.
32394135	1	57	theme	MoS2	238:241	arg1	nanosheets					243:252	MoS2 nanosheets	238:252	MoS2 nanosheets	238:252	A biosensor for hydrogen peroxide (H2O2) has been developed based on the use of MoS2 nanosheets and graphite that are assembled to form a microfiber hybrid structure.
33668381	0	0	theme	Blood	92:96	arg1	Pressure					98:105	Blood Pressure	92:105	Blood Pressure in Healthy Premenopausal Women	92:136	Circulating Levels of the Heparan Sulfate Proteoglycan Syndecan-4 Positively Associate with Blood Pressure in Healthy Premenopausal Women.
33668381	2	1	theme	risk	508:511	arg1	factors					513:519	cardiovascular risk factors	493:519	cardiovascular risk factors in healthy individuals	493:542	Growing evidence suggests that elevated levels of both shed SDC1 and SDC4 are associated with hypertension and cardiovascular diseases, but their relationships with cardiovascular risk factors in healthy individuals are unknown.
33668381	6	2	theme	multivariate	1267:1278	arg1	model					1291:1295	a multivariate regression model	1265:1295	a multivariate regression model that included fat-free mass and urinary dopamine levels as significant independent variables	1265:1388	Serum SDC4 was also a significant predictor of systolic blood pressure in a multivariate regression model that included fat-free mass and urinary dopamine levels as significant independent variables.
33668381	2	3	with	relationships	474:486	arg1	factors					513:519	cardiovascular risk factors	493:519	cardiovascular risk factors in healthy individuals	493:542	Growing evidence suggests that elevated levels of both shed SDC1 and SDC4 are associated with hypertension and cardiovascular diseases, but their relationships with cardiovascular risk factors in healthy individuals are unknown.
33668381	3	4	theme	years	895:899	arg1	ages					877:880	the ages	873:880	the ages of 20 and 40 years old	873:903	The primary objective of this study was to investigate whether serum levels of SDC4 and SDC1 were associated with body composition, hemodynamic parameters, pro-inflammatory cytokine concentrations, and urinary noradrenaline and dopamine levels in healthy women (17 African American and 20 European American) between the ages of 20 and 40 years old.
33668381	5	5	theme	dopamine	1181:1188	arg1	pressure					1131:1138	diastolic blood pressure	1115:1138	diastolic blood pressure	1115:1138	On the other hand, serum SDC4 was positively correlated with systolic blood pressure, diastolic blood pressure, and urinary levels of noradrenaline and dopamine.
33668381	5	5	theme	dopamine	1181:1188	arg1	levels					1153:1158	urinary levels	1145:1158	urinary levels of noradrenaline and dopamine	1145:1188	On the other hand, serum SDC4 was positively correlated with systolic blood pressure, diastolic blood pressure, and urinary levels of noradrenaline and dopamine.
33668381	5	5	theme	dopamine	1181:1188	arg1	pressure					1105:1112	systolic blood pressure	1090:1112	systolic blood pressure	1090:1112	On the other hand, serum SDC4 was positively correlated with systolic blood pressure, diastolic blood pressure, and urinary levels of noradrenaline and dopamine.
33668381	2	6	theme	cardiovascular	493:506	arg1	factors					513:519	cardiovascular risk factors	493:519	cardiovascular risk factors in healthy individuals	493:542	Growing evidence suggests that elevated levels of both shed SDC1 and SDC4 are associated with hypertension and cardiovascular diseases, but their relationships with cardiovascular risk factors in healthy individuals are unknown.
33668381	5	7	theme	diastolic	1115:1123	arg1	pressure					1131:1138	diastolic blood pressure	1115:1138	diastolic blood pressure	1115:1138	On the other hand, serum SDC4 was positively correlated with systolic blood pressure, diastolic blood pressure, and urinary levels of noradrenaline and dopamine.
33668381	6	8	theme	systolic	1238:1245	arg1	pressure					1253:1260	systolic blood pressure	1238:1260	systolic blood pressure	1238:1260	Serum SDC4 was also a significant predictor of systolic blood pressure in a multivariate regression model that included fat-free mass and urinary dopamine levels as significant independent variables.
33668381	1	9	theme	transmembrane	160:172	arg1	Syndecans					139:147	Syndecans	139:147	Syndecans (SDCs)	139:154	Syndecans (SDCs) are transmembrane proteins that are present on most cell types where they play a role in multiple physiological processes, including cell-matrix adhesion and inflammation.
33668381	1	9	theme	transmembrane	160:172	arg1	proteins					174:181	transmembrane proteins	160:181	transmembrane proteins that are present on most cell types where they play a role in multiple physiological processes, including cell-matrix adhesion and inflammation	160:325	Syndecans (SDCs) are transmembrane proteins that are present on most cell types where they play a role in multiple physiological processes, including cell-matrix adhesion and inflammation.
33668381	3	10	theme	20	885:886	arg1	years					895:899	20 and 40 years old	885:903	years	895:899	The primary objective of this study was to investigate whether serum levels of SDC4 and SDC1 were associated with body composition, hemodynamic parameters, pro-inflammatory cytokine concentrations, and urinary noradrenaline and dopamine levels in healthy women (17 African American and 20 European American) between the ages of 20 and 40 years old.
33668381	3	11	dep	composition	676:686	arg1	American					830:837	American	830:837	American	830:837	The primary objective of this study was to investigate whether serum levels of SDC4 and SDC1 were associated with body composition, hemodynamic parameters, pro-inflammatory cytokine concentrations, and urinary noradrenaline and dopamine levels in healthy women (17 African American and 20 European American) between the ages of 20 and 40 years old.
33668381	3	11	dep	composition	676:686	arg1	American					855:862	American	855:862	American	855:862	The primary objective of this study was to investigate whether serum levels of SDC4 and SDC1 were associated with body composition, hemodynamic parameters, pro-inflammatory cytokine concentrations, and urinary noradrenaline and dopamine levels in healthy women (17 African American and 20 European American) between the ages of 20 and 40 years old.
33668381	4	12	theme	p	955:955	arg1	<					957:957	p < 0.05	955:962	p < 0.05	955:962	Univariate analyses revealed only a significant (p < 0.05) inverse correlation between serum SDC1 and body fat percentage.
33668381	5	13	theme	blood	1125:1129	arg1	pressure					1131:1138	diastolic blood pressure	1115:1138	diastolic blood pressure	1115:1138	On the other hand, serum SDC4 was positively correlated with systolic blood pressure, diastolic blood pressure, and urinary levels of noradrenaline and dopamine.
33668381	8	14	theme	physiological	1503:1515	arg1	regulation					1517:1526	the physiological regulation	1499:1526	the physiological regulation of blood pressure	1499:1544	Our findings indicate that SDC4 has an important role in the physiological regulation of blood pressure.
33668381	3	15	theme	primary	561:567	arg1	objective					569:577	The primary objective	557:577	The primary objective of this study	557:591	The primary objective of this study was to investigate whether serum levels of SDC4 and SDC1 were associated with body composition, hemodynamic parameters, pro-inflammatory cytokine concentrations, and urinary noradrenaline and dopamine levels in healthy women (17 African American and 20 European American) between the ages of 20 and 40 years old.
33668381	1	16	theme	multiple	245:252	arg1	processes					268:276	multiple physiological processes	245:276	multiple physiological processes	245:276	Syndecans (SDCs) are transmembrane proteins that are present on most cell types where they play a role in multiple physiological processes, including cell-matrix adhesion and inflammation.
33668381	1	16	theme	multiple	245:252	arg1	inflammation					314:325	inflammation	314:325	inflammation	314:325	Syndecans (SDCs) are transmembrane proteins that are present on most cell types where they play a role in multiple physiological processes, including cell-matrix adhesion and inflammation.
33668381	1	16	theme	multiple	245:252	arg1	adhesion					301:308	cell-matrix adhesion	289:308	cell-matrix adhesion	289:308	Syndecans (SDCs) are transmembrane proteins that are present on most cell types where they play a role in multiple physiological processes, including cell-matrix adhesion and inflammation.
33668381	6	17	theme	pressure	1253:1260	arg1	predictor					1225:1233	a significant predictor	1211:1233	a significant predictor of systolic blood pressure	1211:1260	Serum SDC4 was also a significant predictor of systolic blood pressure in a multivariate regression model that included fat-free mass and urinary dopamine levels as significant independent variables.
33668381	6	17	theme	pressure	1253:1260	arg1	SDC4					1197:1200	Serum SDC4	1191:1200	Serum SDC4	1191:1200	Serum SDC4 was also a significant predictor of systolic blood pressure in a multivariate regression model that included fat-free mass and urinary dopamine levels as significant independent variables.
33668381	1	18	theme	physiological	254:266	arg1	processes					268:276	multiple physiological processes	245:276	multiple physiological processes	245:276	Syndecans (SDCs) are transmembrane proteins that are present on most cell types where they play a role in multiple physiological processes, including cell-matrix adhesion and inflammation.
33668381	1	18	theme	physiological	254:266	arg1	inflammation					314:325	inflammation	314:325	inflammation	314:325	Syndecans (SDCs) are transmembrane proteins that are present on most cell types where they play a role in multiple physiological processes, including cell-matrix adhesion and inflammation.
33668381	1	18	theme	physiological	254:266	arg1	adhesion					301:308	cell-matrix adhesion	289:308	cell-matrix adhesion	289:308	Syndecans (SDCs) are transmembrane proteins that are present on most cell types where they play a role in multiple physiological processes, including cell-matrix adhesion and inflammation.
33668381	0	19	theme	Premenopausal	118:130	arg1	Women					132:136	Healthy Premenopausal Women	110:136	Healthy Premenopausal Women	110:136	Circulating Levels of the Heparan Sulfate Proteoglycan Syndecan-4 Positively Associate with Blood Pressure in Healthy Premenopausal Women.
33668381	3	20	theme	urinary	759:765	arg1	noradrenaline					767:779	urinary noradrenaline and dopamine levels	759:799	noradrenaline	767:779	The primary objective of this study was to investigate whether serum levels of SDC4 and SDC1 were associated with body composition, hemodynamic parameters, pro-inflammatory cytokine concentrations, and urinary noradrenaline and dopamine levels in healthy women (17 African American and 20 European American) between the ages of 20 and 40 years old.
33668381	3	21	from	noradrenaline	767:779	arg1	women					812:816	healthy women	804:816	healthy women	804:816	The primary objective of this study was to investigate whether serum levels of SDC4 and SDC1 were associated with body composition, hemodynamic parameters, pro-inflammatory cytokine concentrations, and urinary noradrenaline and dopamine levels in healthy women (17 African American and 20 European American) between the ages of 20 and 40 years old.
33668381	2	22	theme	SDC4	397:400	arg1	levels					368:373	elevated levels	359:373	elevated levels of both shed SDC1 and SDC4	359:400	Growing evidence suggests that elevated levels of both shed SDC1 and SDC4 are associated with hypertension and cardiovascular diseases, but their relationships with cardiovascular risk factors in healthy individuals are unknown.
33668381	0	23	theme	Healthy	110:116	arg1	Women					132:136	Healthy Premenopausal Women	110:136	Healthy Premenopausal Women	110:136	Circulating Levels of the Heparan Sulfate Proteoglycan Syndecan-4 Positively Associate with Blood Pressure in Healthy Premenopausal Women.
33668381	5	24	theme	systolic	1090:1097	arg1	pressure					1105:1112	systolic blood pressure	1090:1112	systolic blood pressure	1090:1112	On the other hand, serum SDC4 was positively correlated with systolic blood pressure, diastolic blood pressure, and urinary levels of noradrenaline and dopamine.
33668381	3	25	from	parameters	701:710	arg1	women					812:816	healthy women	804:816	healthy women	804:816	The primary objective of this study was to investigate whether serum levels of SDC4 and SDC1 were associated with body composition, hemodynamic parameters, pro-inflammatory cytokine concentrations, and urinary noradrenaline and dopamine levels in healthy women (17 African American and 20 European American) between the ages of 20 and 40 years old.
33668381	4	26	theme	fat	1013:1015	arg1	percentage					1017:1026	body fat percentage	1008:1026	body fat percentage	1008:1026	Univariate analyses revealed only a significant (p < 0.05) inverse correlation between serum SDC1 and body fat percentage.
33668381	3	27	theme	old	901:903	arg1	years					895:899	20 and 40 years old	885:903	years	895:899	The primary objective of this study was to investigate whether serum levels of SDC4 and SDC1 were associated with body composition, hemodynamic parameters, pro-inflammatory cytokine concentrations, and urinary noradrenaline and dopamine levels in healthy women (17 African American and 20 European American) between the ages of 20 and 40 years old.
33668381	3	28	from	concentrations	739:752	arg1	women					812:816	healthy women	804:816	healthy women	804:816	The primary objective of this study was to investigate whether serum levels of SDC4 and SDC1 were associated with body composition, hemodynamic parameters, pro-inflammatory cytokine concentrations, and urinary noradrenaline and dopamine levels in healthy women (17 African American and 20 European American) between the ages of 20 and 40 years old.
33668381	5	29	theme	blood	1099:1103	arg1	pressure					1105:1112	systolic blood pressure	1090:1112	systolic blood pressure	1090:1112	On the other hand, serum SDC4 was positively correlated with systolic blood pressure, diastolic blood pressure, and urinary levels of noradrenaline and dopamine.
33668381	6	30	from	predictor	1225:1233	arg1	model					1291:1295	a multivariate regression model	1265:1295	a multivariate regression model that included fat-free mass and urinary dopamine levels as significant independent variables	1265:1388	Serum SDC4 was also a significant predictor of systolic blood pressure in a multivariate regression model that included fat-free mass and urinary dopamine levels as significant independent variables.
33668381	3	31	theme	study	587:591	arg1	objective					569:577	The primary objective	557:577	The primary objective of this study	557:591	The primary objective of this study was to investigate whether serum levels of SDC4 and SDC1 were associated with body composition, hemodynamic parameters, pro-inflammatory cytokine concentrations, and urinary noradrenaline and dopamine levels in healthy women (17 African American and 20 European American) between the ages of 20 and 40 years old.
33668381	5	32	theme	urinary	1145:1151	arg1	levels					1153:1158	urinary levels	1145:1158	urinary levels of noradrenaline and dopamine	1145:1188	On the other hand, serum SDC4 was positively correlated with systolic blood pressure, diastolic blood pressure, and urinary levels of noradrenaline and dopamine.
33668381	2	33	theme	SDC1	388:391	arg1	levels					368:373	elevated levels	359:373	elevated levels of both shed SDC1 and SDC4	359:400	Growing evidence suggests that elevated levels of both shed SDC1 and SDC4 are associated with hypertension and cardiovascular diseases, but their relationships with cardiovascular risk factors in healthy individuals are unknown.
33668381	0	34	theme	Circulating	0:10	arg1	Levels					12:17	Circulating Levels	0:17	Circulating Levels of the Heparan Sulfate Proteoglycan Syndecan-4	0:64	Circulating Levels of the Heparan Sulfate Proteoglycan Syndecan-4 Positively Associate with Blood Pressure in Healthy Premenopausal Women.
33668381	3	35	theme	dopamine	785:792	arg1	levels					794:799	urinary noradrenaline and dopamine levels	759:799	levels	794:799	The primary objective of this study was to investigate whether serum levels of SDC4 and SDC1 were associated with body composition, hemodynamic parameters, pro-inflammatory cytokine concentrations, and urinary noradrenaline and dopamine levels in healthy women (17 African American and 20 European American) between the ages of 20 and 40 years old.
33668381	3	36	from	levels	794:799	arg1	women					812:816	healthy women	804:816	healthy women	804:816	The primary objective of this study was to investigate whether serum levels of SDC4 and SDC1 were associated with body composition, hemodynamic parameters, pro-inflammatory cytokine concentrations, and urinary noradrenaline and dopamine levels in healthy women (17 African American and 20 European American) between the ages of 20 and 40 years old.
33668381	4	37	theme	body	1008:1011	arg1	percentage					1017:1026	body fat percentage	1008:1026	body fat percentage	1008:1026	Univariate analyses revealed only a significant (p < 0.05) inverse correlation between serum SDC1 and body fat percentage.
33668381	6	38	theme	blood	1247:1251	arg1	pressure					1253:1260	systolic blood pressure	1238:1260	systolic blood pressure	1238:1260	Serum SDC4 was also a significant predictor of systolic blood pressure in a multivariate regression model that included fat-free mass and urinary dopamine levels as significant independent variables.
33668381	2	39	theme	shed	383:386	arg1	SDC1					388:391	shed SDC1	383:391	shed SDC1	383:391	Growing evidence suggests that elevated levels of both shed SDC1 and SDC4 are associated with hypertension and cardiovascular diseases, but their relationships with cardiovascular risk factors in healthy individuals are unknown.
33668381	2	40	theme	cardiovascular	439:452	arg1	diseases					454:461	cardiovascular diseases	439:461	cardiovascular diseases	439:461	Growing evidence suggests that elevated levels of both shed SDC1 and SDC4 are associated with hypertension and cardiovascular diseases, but their relationships with cardiovascular risk factors in healthy individuals are unknown.
33668381	8	41	theme	pressure	1537:1544	arg1	regulation					1517:1526	the physiological regulation	1499:1526	the physiological regulation of blood pressure	1499:1544	Our findings indicate that SDC4 has an important role in the physiological regulation of blood pressure.
33668381	6	42	theme	dopamine	1337:1344	arg1	variables					1380:1388	significant independent variables	1356:1388	significant independent variables	1356:1388	Serum SDC4 was also a significant predictor of systolic blood pressure in a multivariate regression model that included fat-free mass and urinary dopamine levels as significant independent variables.
33668381	6	42	theme	dopamine	1337:1344	arg1	levels					1346:1351	urinary dopamine levels	1329:1351	urinary dopamine levels	1329:1351	Serum SDC4 was also a significant predictor of systolic blood pressure in a multivariate regression model that included fat-free mass and urinary dopamine levels as significant independent variables.
33668381	6	42	theme	dopamine	1337:1344	arg1	mass					1320:1323	fat-free mass	1311:1323	fat-free mass	1311:1323	Serum SDC4 was also a significant predictor of systolic blood pressure in a multivariate regression model that included fat-free mass and urinary dopamine levels as significant independent variables.
33668381	6	43	theme	independent	1368:1378	arg1	variables					1380:1388	significant independent variables	1356:1388	significant independent variables	1356:1388	Serum SDC4 was also a significant predictor of systolic blood pressure in a multivariate regression model that included fat-free mass and urinary dopamine levels as significant independent variables.
33668381	6	43	theme	independent	1368:1378	arg1	levels					1346:1351	urinary dopamine levels	1329:1351	urinary dopamine levels	1329:1351	Serum SDC4 was also a significant predictor of systolic blood pressure in a multivariate regression model that included fat-free mass and urinary dopamine levels as significant independent variables.
33668381	6	43	theme	independent	1368:1378	arg1	mass					1320:1323	fat-free mass	1311:1323	fat-free mass	1311:1323	Serum SDC4 was also a significant predictor of systolic blood pressure in a multivariate regression model that included fat-free mass and urinary dopamine levels as significant independent variables.
33668381	5	44	theme	noradrenaline	1163:1175	arg1	pressure					1131:1138	diastolic blood pressure	1115:1138	diastolic blood pressure	1115:1138	On the other hand, serum SDC4 was positively correlated with systolic blood pressure, diastolic blood pressure, and urinary levels of noradrenaline and dopamine.
33668381	5	44	theme	noradrenaline	1163:1175	arg1	levels					1153:1158	urinary levels	1145:1158	urinary levels of noradrenaline and dopamine	1145:1188	On the other hand, serum SDC4 was positively correlated with systolic blood pressure, diastolic blood pressure, and urinary levels of noradrenaline and dopamine.
33668381	5	44	theme	noradrenaline	1163:1175	arg1	pressure					1105:1112	systolic blood pressure	1090:1112	systolic blood pressure	1090:1112	On the other hand, serum SDC4 was positively correlated with systolic blood pressure, diastolic blood pressure, and urinary levels of noradrenaline and dopamine.
33668381	5	45	theme	serum	1048:1052	arg1	SDC4					1054:1057	serum SDC4	1048:1057	serum SDC4	1048:1057	On the other hand, serum SDC4 was positively correlated with systolic blood pressure, diastolic blood pressure, and urinary levels of noradrenaline and dopamine.
33668381	2	46	theme	elevated	359:366	arg1	levels					368:373	elevated levels	359:373	elevated levels of both shed SDC1 and SDC4	359:400	Growing evidence suggests that elevated levels of both shed SDC1 and SDC4 are associated with hypertension and cardiovascular diseases, but their relationships with cardiovascular risk factors in healthy individuals are unknown.
33668381	3	47	theme	SDC1	645:648	arg1	levels					626:631	serum levels	620:631	serum levels of SDC4 and SDC1	620:648	The primary objective of this study was to investigate whether serum levels of SDC4 and SDC1 were associated with body composition, hemodynamic parameters, pro-inflammatory cytokine concentrations, and urinary noradrenaline and dopamine levels in healthy women (17 African American and 20 European American) between the ages of 20 and 40 years old.
33668381	1	48	theme	most	203:206	arg1	types					213:217	most cell types	203:217	most cell types where they play a role in multiple physiological processes, including cell-matrix adhesion and inflammation	203:325	Syndecans (SDCs) are transmembrane proteins that are present on most cell types where they play a role in multiple physiological processes, including cell-matrix adhesion and inflammation.
33668381	0	49	theme	Sulfate	34:40	arg1	Syndecan-4					55:64	the Heparan Sulfate Proteoglycan Syndecan-4	22:64	the Heparan Sulfate Proteoglycan Syndecan-4	22:64	Circulating Levels of the Heparan Sulfate Proteoglycan Syndecan-4 Positively Associate with Blood Pressure in Healthy Premenopausal Women.
33668381	1	50	theme	cell-matrix	289:299	arg1	adhesion					301:308	cell-matrix adhesion	289:308	cell-matrix adhesion	289:308	Syndecans (SDCs) are transmembrane proteins that are present on most cell types where they play a role in multiple physiological processes, including cell-matrix adhesion and inflammation.
33668381	4	51	theme	significant	942:952	arg1	correlation					973:983	a significant (p < 0.05) inverse correlation	940:983	a significant (p < 0.05) inverse correlation between serum SDC1 and body fat percentage	940:1026	Univariate analyses revealed only a significant (p < 0.05) inverse correlation between serum SDC1 and body fat percentage.
33668381	6	52	theme	significant	1213:1223	arg1	predictor					1225:1233	a significant predictor	1211:1233	a significant predictor of systolic blood pressure	1211:1260	Serum SDC4 was also a significant predictor of systolic blood pressure in a multivariate regression model that included fat-free mass and urinary dopamine levels as significant independent variables.
33668381	6	52	theme	significant	1213:1223	arg1	SDC4					1197:1200	Serum SDC4	1191:1200	Serum SDC4	1191:1200	Serum SDC4 was also a significant predictor of systolic blood pressure in a multivariate regression model that included fat-free mass and urinary dopamine levels as significant independent variables.
33668381	1	53	theme	cell	208:211	arg1	types					213:217	most cell types	203:217	most cell types where they play a role in multiple physiological processes, including cell-matrix adhesion and inflammation	203:325	Syndecans (SDCs) are transmembrane proteins that are present on most cell types where they play a role in multiple physiological processes, including cell-matrix adhesion and inflammation.
33668381	0	54	theme	Heparan	26:32	arg1	Syndecan-4					55:64	the Heparan Sulfate Proteoglycan Syndecan-4	22:64	the Heparan Sulfate Proteoglycan Syndecan-4	22:64	Circulating Levels of the Heparan Sulfate Proteoglycan Syndecan-4 Positively Associate with Blood Pressure in Healthy Premenopausal Women.
33668381	4	55	theme	serum	993:997	arg1	SDC1					999:1002	serum SDC1	993:1002	serum SDC1	993:1002	Univariate analyses revealed only a significant (p < 0.05) inverse correlation between serum SDC1 and body fat percentage.
33668381	1	56	located	present	192:198	arg2	proteins					174:181	transmembrane proteins	160:181	transmembrane proteins that are present on most cell types where they play a role in multiple physiological processes, including cell-matrix adhesion and inflammation	160:325	Syndecans (SDCs) are transmembrane proteins that are present on most cell types where they play a role in multiple physiological processes, including cell-matrix adhesion and inflammation.
33668381	1	56	located	present	192:198	arg2	Syndecans					139:147	Syndecans	139:147	Syndecans (SDCs)	139:154	Syndecans (SDCs) are transmembrane proteins that are present on most cell types where they play a role in multiple physiological processes, including cell-matrix adhesion and inflammation.
33668381	1	56	located	present	192:198	arg1	types					213:217	most cell types	203:217	most cell types where they play a role in multiple physiological processes, including cell-matrix adhesion and inflammation	203:325	Syndecans (SDCs) are transmembrane proteins that are present on most cell types where they play a role in multiple physiological processes, including cell-matrix adhesion and inflammation.
33668381	3	57	theme	serum	620:624	arg1	levels					626:631	serum levels	620:631	serum levels of SDC4 and SDC1	620:648	The primary objective of this study was to investigate whether serum levels of SDC4 and SDC1 were associated with body composition, hemodynamic parameters, pro-inflammatory cytokine concentrations, and urinary noradrenaline and dopamine levels in healthy women (17 African American and 20 European American) between the ages of 20 and 40 years old.
33668381	2	58	theme	healthy	524:530	arg1	individuals					532:542	healthy individuals	524:542	healthy individuals	524:542	Growing evidence suggests that elevated levels of both shed SDC1 and SDC4 are associated with hypertension and cardiovascular diseases, but their relationships with cardiovascular risk factors in healthy individuals are unknown.
33668381	0	59	theme	Syndecan-4	55:64	arg1	Levels					12:17	Circulating Levels	0:17	Circulating Levels of the Heparan Sulfate Proteoglycan Syndecan-4	0:64	Circulating Levels of the Heparan Sulfate Proteoglycan Syndecan-4 Positively Associate with Blood Pressure in Healthy Premenopausal Women.
33668381	4	60	dep	significant	942:952	arg1	<					957:957	p < 0.05	955:962	p < 0.05	955:962	Univariate analyses revealed only a significant (p < 0.05) inverse correlation between serum SDC1 and body fat percentage.
33668381	6	61	theme	significant	1356:1366	arg1	variables					1380:1388	significant independent variables	1356:1388	significant independent variables	1356:1388	Serum SDC4 was also a significant predictor of systolic blood pressure in a multivariate regression model that included fat-free mass and urinary dopamine levels as significant independent variables.
33668381	6	61	theme	significant	1356:1366	arg1	levels					1346:1351	urinary dopamine levels	1329:1351	urinary dopamine levels	1329:1351	Serum SDC4 was also a significant predictor of systolic blood pressure in a multivariate regression model that included fat-free mass and urinary dopamine levels as significant independent variables.
33668381	6	61	theme	significant	1356:1366	arg1	mass					1320:1323	fat-free mass	1311:1323	fat-free mass	1311:1323	Serum SDC4 was also a significant predictor of systolic blood pressure in a multivariate regression model that included fat-free mass and urinary dopamine levels as significant independent variables.
33668381	2	62	from	factors	513:519	arg1	individuals					532:542	healthy individuals	524:542	healthy individuals	524:542	Growing evidence suggests that elevated levels of both shed SDC1 and SDC4 are associated with hypertension and cardiovascular diseases, but their relationships with cardiovascular risk factors in healthy individuals are unknown.
33668381	8	63	theme	important	1481:1489	arg1	role					1491:1494	an important role	1478:1494	an important role	1478:1494	Our findings indicate that SDC4 has an important role in the physiological regulation of blood pressure.
33668381	8	64	theme	blood	1531:1535	arg1	pressure					1537:1544	blood pressure	1531:1544	blood pressure	1531:1544	Our findings indicate that SDC4 has an important role in the physiological regulation of blood pressure.
33668381	0	65	theme	Proteoglycan	42:53	arg1	Syndecan-4					55:64	the Heparan Sulfate Proteoglycan Syndecan-4	22:64	the Heparan Sulfate Proteoglycan Syndecan-4	22:64	Circulating Levels of the Heparan Sulfate Proteoglycan Syndecan-4 Positively Associate with Blood Pressure in Healthy Premenopausal Women.
33668381	6	66	theme	urinary	1329:1335	arg1	variables					1380:1388	significant independent variables	1356:1388	significant independent variables	1356:1388	Serum SDC4 was also a significant predictor of systolic blood pressure in a multivariate regression model that included fat-free mass and urinary dopamine levels as significant independent variables.
33668381	6	66	theme	urinary	1329:1335	arg1	levels					1346:1351	urinary dopamine levels	1329:1351	urinary dopamine levels	1329:1351	Serum SDC4 was also a significant predictor of systolic blood pressure in a multivariate regression model that included fat-free mass and urinary dopamine levels as significant independent variables.
33668381	6	66	theme	urinary	1329:1335	arg1	mass					1320:1323	fat-free mass	1311:1323	fat-free mass	1311:1323	Serum SDC4 was also a significant predictor of systolic blood pressure in a multivariate regression model that included fat-free mass and urinary dopamine levels as significant independent variables.
33668381	5	67	theme	other	1036:1040	arg1	hand					1042:1045	the other hand	1032:1045	the other hand	1032:1045	On the other hand, serum SDC4 was positively correlated with systolic blood pressure, diastolic blood pressure, and urinary levels of noradrenaline and dopamine.
33668381	1	68	attach	present	192:198	arg2	proteins					174:181	transmembrane proteins	160:181	transmembrane proteins that are present on most cell types where they play a role in multiple physiological processes, including cell-matrix adhesion and inflammation	160:325	Syndecans (SDCs) are transmembrane proteins that are present on most cell types where they play a role in multiple physiological processes, including cell-matrix adhesion and inflammation.
33668381	1	68	attach	present	192:198	arg2	Syndecans					139:147	Syndecans	139:147	Syndecans (SDCs)	139:154	Syndecans (SDCs) are transmembrane proteins that are present on most cell types where they play a role in multiple physiological processes, including cell-matrix adhesion and inflammation.
33668381	1	68	attach	present	192:198	arg1	types					213:217	most cell types	203:217	most cell types where they play a role in multiple physiological processes, including cell-matrix adhesion and inflammation	203:325	Syndecans (SDCs) are transmembrane proteins that are present on most cell types where they play a role in multiple physiological processes, including cell-matrix adhesion and inflammation.
33668381	8	69	contain	has	1474:1476	arg1	SDC4					1469:1472	SDC4	1469:1472	SDC4	1469:1472	Our findings indicate that SDC4 has an important role in the physiological regulation of blood pressure.
33668381	8	69	contain	has	1474:1476	arg2	role					1491:1494	an important role	1478:1494	an important role	1478:1494	Our findings indicate that SDC4 has an important role in the physiological regulation of blood pressure.
33668381	3	70	theme	healthy	804:810	arg1	women					812:816	healthy women	804:816	healthy women	804:816	The primary objective of this study was to investigate whether serum levels of SDC4 and SDC1 were associated with body composition, hemodynamic parameters, pro-inflammatory cytokine concentrations, and urinary noradrenaline and dopamine levels in healthy women (17 African American and 20 European American) between the ages of 20 and 40 years old.
33668381	3	71	theme	hemodynamic	689:699	arg1	parameters					701:710	hemodynamic parameters	689:710	hemodynamic parameters	689:710	The primary objective of this study was to investigate whether serum levels of SDC4 and SDC1 were associated with body composition, hemodynamic parameters, pro-inflammatory cytokine concentrations, and urinary noradrenaline and dopamine levels in healthy women (17 African American and 20 European American) between the ages of 20 and 40 years old.
33668381	3	72	theme	SDC4	636:639	arg1	levels					626:631	serum levels	620:631	serum levels of SDC4 and SDC1	620:648	The primary objective of this study was to investigate whether serum levels of SDC4 and SDC1 were associated with body composition, hemodynamic parameters, pro-inflammatory cytokine concentrations, and urinary noradrenaline and dopamine levels in healthy women (17 African American and 20 European American) between the ages of 20 and 40 years old.
33668381	6	73	theme	fat-free	1311:1318	arg1	mass					1320:1323	fat-free mass	1311:1323	fat-free mass	1311:1323	Serum SDC4 was also a significant predictor of systolic blood pressure in a multivariate regression model that included fat-free mass and urinary dopamine levels as significant independent variables.
33668381	6	73	theme	fat-free	1311:1318	arg1	levels					1346:1351	urinary dopamine levels	1329:1351	urinary dopamine levels	1329:1351	Serum SDC4 was also a significant predictor of systolic blood pressure in a multivariate regression model that included fat-free mass and urinary dopamine levels as significant independent variables.
33668381	6	73	theme	fat-free	1311:1318	arg1	variables					1380:1388	significant independent variables	1356:1388	significant independent variables	1356:1388	Serum SDC4 was also a significant predictor of systolic blood pressure in a multivariate regression model that included fat-free mass and urinary dopamine levels as significant independent variables.
33668381	4	74	theme	Univariate	906:915	arg1	analyses					917:924	Univariate analyses	906:924	Univariate analyses	906:924	Univariate analyses revealed only a significant (p < 0.05) inverse correlation between serum SDC1 and body fat percentage.
33668381	3	75	theme	body	671:674	arg1	composition					676:686	body composition	671:686	body composition	671:686	The primary objective of this study was to investigate whether serum levels of SDC4 and SDC1 were associated with body composition, hemodynamic parameters, pro-inflammatory cytokine concentrations, and urinary noradrenaline and dopamine levels in healthy women (17 African American and 20 European American) between the ages of 20 and 40 years old.
33668381	6	76	theme	Serum	1191:1195	arg1	predictor					1225:1233	a significant predictor	1211:1233	a significant predictor of systolic blood pressure	1211:1260	Serum SDC4 was also a significant predictor of systolic blood pressure in a multivariate regression model that included fat-free mass and urinary dopamine levels as significant independent variables.
33668381	6	76	theme	Serum	1191:1195	arg1	SDC4					1197:1200	Serum SDC4	1191:1200	Serum SDC4	1191:1200	Serum SDC4 was also a significant predictor of systolic blood pressure in a multivariate regression model that included fat-free mass and urinary dopamine levels as significant independent variables.
33668381	2	77	theme	Growing	328:334	arg1	evidence					336:343	Growing evidence	328:343	Growing evidence	328:343	Growing evidence suggests that elevated levels of both shed SDC1 and SDC4 are associated with hypertension and cardiovascular diseases, but their relationships with cardiovascular risk factors in healthy individuals are unknown.
33668381	6	78	theme	regression	1280:1289	arg1	model					1291:1295	a multivariate regression model	1265:1295	a multivariate regression model that included fat-free mass and urinary dopamine levels as significant independent variables	1265:1388	Serum SDC4 was also a significant predictor of systolic blood pressure in a multivariate regression model that included fat-free mass and urinary dopamine levels as significant independent variables.
33668381	4	79	theme	inverse	965:971	arg1	correlation					973:983	a significant (p < 0.05) inverse correlation	940:983	a significant (p < 0.05) inverse correlation between serum SDC1 and body fat percentage	940:1026	Univariate analyses revealed only a significant (p < 0.05) inverse correlation between serum SDC1 and body fat percentage.
33668381	0	80	from	Pressure	98:105	arg1	Women					132:136	Healthy Premenopausal Women	110:136	Healthy Premenopausal Women	110:136	Circulating Levels of the Heparan Sulfate Proteoglycan Syndecan-4 Positively Associate with Blood Pressure in Healthy Premenopausal Women.
33668381	3	81	from	composition	676:686	arg1	women					812:816	healthy women	804:816	healthy women	804:816	The primary objective of this study was to investigate whether serum levels of SDC4 and SDC1 were associated with body composition, hemodynamic parameters, pro-inflammatory cytokine concentrations, and urinary noradrenaline and dopamine levels in healthy women (17 African American and 20 European American) between the ages of 20 and 40 years old.
33668381	3	82	theme	pro-inflammatory	713:728	arg1	concentrations					739:752	pro-inflammatory cytokine concentrations	713:752	pro-inflammatory cytokine concentrations	713:752	The primary objective of this study was to investigate whether serum levels of SDC4 and SDC1 were associated with body composition, hemodynamic parameters, pro-inflammatory cytokine concentrations, and urinary noradrenaline and dopamine levels in healthy women (17 African American and 20 European American) between the ages of 20 and 40 years old.
33668381	3	83	theme	cytokine	730:737	arg1	concentrations					739:752	pro-inflammatory cytokine concentrations	713:752	pro-inflammatory cytokine concentrations	713:752	The primary objective of this study was to investigate whether serum levels of SDC4 and SDC1 were associated with body composition, hemodynamic parameters, pro-inflammatory cytokine concentrations, and urinary noradrenaline and dopamine levels in healthy women (17 African American and 20 European American) between the ages of 20 and 40 years old.
33668381	3	84	theme	40	892:893	arg1	years					895:899	20 and 40 years old	885:903	years	895:899	The primary objective of this study was to investigate whether serum levels of SDC4 and SDC1 were associated with body composition, hemodynamic parameters, pro-inflammatory cytokine concentrations, and urinary noradrenaline and dopamine levels in healthy women (17 African American and 20 European American) between the ages of 20 and 40 years old.
33151233	3	0	theme	diet-induced	850:861	arg1	gain					870:873	high fat diet-induced weight gain	841:873	high fat diet-induced weight gain	841:873	The results indicated that oral administration of MLDs markedly reduced high fat diet-induced weight gain and white adipose tissue weights and reversed glucose tolerance, especially at high doses.
33151233	8	1	theme	bacteria	1743:1750	arg1	number					1718:1723	the number	1714:1723	the number of SCFA-producing bacteria (Bacteroidaceae)	1714:1767	Intervention with MLDs modified the gut microbiota in mice with HFD-induced obesity, increasing the number of SCFA-producing bacteria (Bacteroidaceae) and reducing opportunistic pathogens (Enterobacteriaceae and Desulfovibrionaceae).
33151233	1	2	theme	MLDs	371:374	arg1	administration					353:366	oral administration	348:366	oral administration of MLDs	348:374	The objective of this study is to assess the potential anti-obesity effects of black garlic melanoidins (MLDs) and gut microbiota changes in an animal model, hypothesizing that the effects of oral administration of MLDs can be partially mediated by the modulation of intestinal microbiota via inhibiting the formation of lipopolysaccharides (LPS) and promoting the production of short-chain fatty acids (SCFAs).
33151233	6	3	theme	triacylglyceride	1349:1364	arg1	lipase					1366:1371	adipose triacylglyceride lipase	1341:1371	adipose triacylglyceride lipase (ATGL)	1341:1378	The MLDs could down-regulate the expression of fatty acid synthase (FAS) and interleukin-6 (IL-6) and up-regulate the expression of adipose triacylglyceride lipase (ATGL) and hormone sensitive lipase (HSL) in adipose tissues and livers at mRNA levels.
33151233	6	3	theme	triacylglyceride	1349:1364	arg1	ATGL					1374:1377	ATGL	1374:1377	ATGL	1374:1377	The MLDs could down-regulate the expression of fatty acid synthase (FAS) and interleukin-6 (IL-6) and up-regulate the expression of adipose triacylglyceride lipase (ATGL) and hormone sensitive lipase (HSL) in adipose tissues and livers at mRNA levels.
33151233	1	4	theme	garlic	241:246	arg1	MLDs					261:264	MLDs	261:264	MLDs	261:264	The objective of this study is to assess the potential anti-obesity effects of black garlic melanoidins (MLDs) and gut microbiota changes in an animal model, hypothesizing that the effects of oral administration of MLDs can be partially mediated by the modulation of intestinal microbiota via inhibiting the formation of lipopolysaccharides (LPS) and promoting the production of short-chain fatty acids (SCFAs).
33151233	1	4	theme	garlic	241:246	arg1	melanoidins					248:258	black garlic melanoidins	235:258	black garlic melanoidins (MLDs)	235:265	The objective of this study is to assess the potential anti-obesity effects of black garlic melanoidins (MLDs) and gut microbiota changes in an animal model, hypothesizing that the effects of oral administration of MLDs can be partially mediated by the modulation of intestinal microbiota via inhibiting the formation of lipopolysaccharides (LPS) and promoting the production of short-chain fatty acids (SCFAs).
33151233	2	5	theme	kg-1	721:724	arg1	day-1					726:730	kg-1 day-1	721:730	100 mg kg-1 day-1	714:730	The effects of MLDs in C57BL/6J mice with high-fat diet (HFD)-induced obesity were investigated for 12 weeks with low (50 mg kg-1 day-1), medium (100 mg kg-1 day-1) and high (200 mg kg-1 day-1) doses.
33151233	1	6	from	changes	286:292	arg1	model					307:311	an animal model	297:311	an animal model	297:311	The objective of this study is to assess the potential anti-obesity effects of black garlic melanoidins (MLDs) and gut microbiota changes in an animal model, hypothesizing that the effects of oral administration of MLDs can be partially mediated by the modulation of intestinal microbiota via inhibiting the formation of lipopolysaccharides (LPS) and promoting the production of short-chain fatty acids (SCFAs).
33151233	3	7	theme	white	879:883	arg1	tissue					893:898	white adipose tissue	879:898	white adipose tissue	879:898	The results indicated that oral administration of MLDs markedly reduced high fat diet-induced weight gain and white adipose tissue weights and reversed glucose tolerance, especially at high doses.
33151233	1	8	theme	intestinal	423:432	arg1	microbiota					434:443	intestinal microbiota	423:443	intestinal microbiota	423:443	The objective of this study is to assess the potential anti-obesity effects of black garlic melanoidins (MLDs) and gut microbiota changes in an animal model, hypothesizing that the effects of oral administration of MLDs can be partially mediated by the modulation of intestinal microbiota via inhibiting the formation of lipopolysaccharides (LPS) and promoting the production of short-chain fatty acids (SCFAs).
33151233	3	9	theme	glucose	921:927	arg1	tolerance					929:937	glucose tolerance	921:937	glucose tolerance	921:937	The results indicated that oral administration of MLDs markedly reduced high fat diet-induced weight gain and white adipose tissue weights and reversed glucose tolerance, especially at high doses.
33151233	8	10	theme	opportunistic	1782:1794	arg1	Desulfovibrionaceae					1830:1848	Desulfovibrionaceae	1830:1848	Desulfovibrionaceae	1830:1848	Intervention with MLDs modified the gut microbiota in mice with HFD-induced obesity, increasing the number of SCFA-producing bacteria (Bacteroidaceae) and reducing opportunistic pathogens (Enterobacteriaceae and Desulfovibrionaceae).
33151233	8	10	theme	opportunistic	1782:1794	arg1	Enterobacteriaceae					1807:1824	Enterobacteriaceae	1807:1824	Enterobacteriaceae	1807:1824	Intervention with MLDs modified the gut microbiota in mice with HFD-induced obesity, increasing the number of SCFA-producing bacteria (Bacteroidaceae) and reducing opportunistic pathogens (Enterobacteriaceae and Desulfovibrionaceae).
33151233	8	10	theme	opportunistic	1782:1794	arg1	pathogens					1796:1804	opportunistic pathogens	1782:1804	opportunistic pathogens (Enterobacteriaceae and Desulfovibrionaceae)	1782:1849	Intervention with MLDs modified the gut microbiota in mice with HFD-induced obesity, increasing the number of SCFA-producing bacteria (Bacteroidaceae) and reducing opportunistic pathogens (Enterobacteriaceae and Desulfovibrionaceae).
33151233	1	11	theme	study	178:182	arg1	objective					160:168	The objective	156:168	The objective of this study	156:182	The objective of this study is to assess the potential anti-obesity effects of black garlic melanoidins (MLDs) and gut microbiota changes in an animal model, hypothesizing that the effects of oral administration of MLDs can be partially mediated by the modulation of intestinal microbiota via inhibiting the formation of lipopolysaccharides (LPS) and promoting the production of short-chain fatty acids (SCFAs).
33151233	1	12	theme	oral	348:351	arg1	administration					353:366	oral administration	348:366	oral administration of MLDs	348:374	The objective of this study is to assess the potential anti-obesity effects of black garlic melanoidins (MLDs) and gut microbiota changes in an animal model, hypothesizing that the effects of oral administration of MLDs can be partially mediated by the modulation of intestinal microbiota via inhibiting the formation of lipopolysaccharides (LPS) and promoting the production of short-chain fatty acids (SCFAs).
33151233	6	13	theme	lipase	1402:1407	arg1	expression					1327:1336	the expression	1323:1336	the expression of adipose triacylglyceride lipase (ATGL) and hormone sensitive lipase (HSL) in adipose tissues and livers at mRNA levels	1323:1458	The MLDs could down-regulate the expression of fatty acid synthase (FAS) and interleukin-6 (IL-6) and up-regulate the expression of adipose triacylglyceride lipase (ATGL) and hormone sensitive lipase (HSL) in adipose tissues and livers at mRNA levels.
33151233	7	14	theme	MLDs	1504:1507	arg1	administration					1486:1499	the oral administration	1477:1499	the oral administration of MLDs	1477:1507	Moreover, after the oral administration of MLDs, the intestinal microbial environment improved in the sense that bacterial diversity and richness increased.
33151233	2	15	theme	kg-1	693:696	arg1	day-1					698:702	kg-1 day-1	693:702	50 mg kg-1 day-1	687:702	The effects of MLDs in C57BL/6J mice with high-fat diet (HFD)-induced obesity were investigated for 12 weeks with low (50 mg kg-1 day-1), medium (100 mg kg-1 day-1) and high (200 mg kg-1 day-1) doses.
33151233	8	16	with	mice	1672:1675	arg1	obesity					1694:1700	HFD-induced obesity	1682:1700	HFD-induced obesity	1682:1700	Intervention with MLDs modified the gut microbiota in mice with HFD-induced obesity, increasing the number of SCFA-producing bacteria (Bacteroidaceae) and reducing opportunistic pathogens (Enterobacteriaceae and Desulfovibrionaceae).
33151233	0	17	theme	diet-induced	122:133	arg1	mice					150:153	high-fat diet-induced obese C57BL/6J mice	113:153	high-fat diet-induced obese C57BL/6J mice	113:153	Black garlic melanoidins prevent obesity, reduce serum LPS levels and modulate the gut microbiota composition in high-fat diet-induced obese C57BL/6J mice.
33151233	2	18	from	mice	600:603	arg1	effects					572:578	The effects	568:578	The effects of MLDs in C57BL/6J mice with high-fat diet (HFD)-induced obesity	568:644	The effects of MLDs in C57BL/6J mice with high-fat diet (HFD)-induced obesity were investigated for 12 weeks with low (50 mg kg-1 day-1), medium (100 mg kg-1 day-1) and high (200 mg kg-1 day-1) doses.
33151233	0	19	theme	C57BL/6J	141:148	arg1	mice					150:153	high-fat diet-induced obese C57BL/6J mice	113:153	high-fat diet-induced obese C57BL/6J mice	113:153	Black garlic melanoidins prevent obesity, reduce serum LPS levels and modulate the gut microbiota composition in high-fat diet-induced obese C57BL/6J mice.
33151233	8	20	theme	gut	1654:1656	arg1	microbiota					1658:1667	the gut microbiota	1650:1667	the gut microbiota in mice with HFD-induced obesity	1650:1700	Intervention with MLDs modified the gut microbiota in mice with HFD-induced obesity, increasing the number of SCFA-producing bacteria (Bacteroidaceae) and reducing opportunistic pathogens (Enterobacteriaceae and Desulfovibrionaceae).
33151233	6	21	from	levels	1453:1458	arg1	expression					1327:1336	the expression	1323:1336	the expression of adipose triacylglyceride lipase (ATGL) and hormone sensitive lipase (HSL) in adipose tissues and livers at mRNA levels	1323:1458	The MLDs could down-regulate the expression of fatty acid synthase (FAS) and interleukin-6 (IL-6) and up-regulate the expression of adipose triacylglyceride lipase (ATGL) and hormone sensitive lipase (HSL) in adipose tissues and livers at mRNA levels.
33151233	6	21	from	levels	1453:1458	arg1	livers					1438:1443	livers	1438:1443	livers at mRNA levels	1438:1458	The MLDs could down-regulate the expression of fatty acid synthase (FAS) and interleukin-6 (IL-6) and up-regulate the expression of adipose triacylglyceride lipase (ATGL) and hormone sensitive lipase (HSL) in adipose tissues and livers at mRNA levels.
33151233	6	21	from	levels	1453:1458	arg1	tissues					1426:1432	adipose tissues	1418:1432	adipose tissues	1418:1432	The MLDs could down-regulate the expression of fatty acid synthase (FAS) and interleukin-6 (IL-6) and up-regulate the expression of adipose triacylglyceride lipase (ATGL) and hormone sensitive lipase (HSL) in adipose tissues and livers at mRNA levels.
33151233	2	22	theme	-induced	629:636	arg1	obesity					638:644	high-fat diet (HFD)-induced obesity	610:644	high-fat diet (HFD)-induced obesity	610:644	The effects of MLDs in C57BL/6J mice with high-fat diet (HFD)-induced obesity were investigated for 12 weeks with low (50 mg kg-1 day-1), medium (100 mg kg-1 day-1) and high (200 mg kg-1 day-1) doses.
33151233	9	23	theme	probiotics	1884:1893	arg1	abundance					1865:1873	An increased abundance	1852:1873	An increased abundance of other probiotics including Lactobacillaceae and Akkermansiaceae	1852:1940	An increased abundance of other probiotics including Lactobacillaceae and Akkermansiaceae was also observed.
33151233	6	24	theme	mRNA	1448:1451	arg1	levels					1453:1458	mRNA levels	1448:1458	mRNA levels	1448:1458	The MLDs could down-regulate the expression of fatty acid synthase (FAS) and interleukin-6 (IL-6) and up-regulate the expression of adipose triacylglyceride lipase (ATGL) and hormone sensitive lipase (HSL) in adipose tissues and livers at mRNA levels.
33151233	8	25	dep	pathogens	1796:1804	arg1	Desulfovibrionaceae					1830:1848	Desulfovibrionaceae	1830:1848	Desulfovibrionaceae	1830:1848	Intervention with MLDs modified the gut microbiota in mice with HFD-induced obesity, increasing the number of SCFA-producing bacteria (Bacteroidaceae) and reducing opportunistic pathogens (Enterobacteriaceae and Desulfovibrionaceae).
33151233	8	25	dep	pathogens	1796:1804	arg1	Enterobacteriaceae					1807:1824	Enterobacteriaceae	1807:1824	Enterobacteriaceae	1807:1824	Intervention with MLDs modified the gut microbiota in mice with HFD-induced obesity, increasing the number of SCFA-producing bacteria (Bacteroidaceae) and reducing opportunistic pathogens (Enterobacteriaceae and Desulfovibrionaceae).
33151233	8	25	dep	pathogens	1796:1804	arg1	pathogens					1796:1804	opportunistic pathogens	1782:1804	opportunistic pathogens (Enterobacteriaceae and Desulfovibrionaceae)	1782:1849	Intervention with MLDs modified the gut microbiota in mice with HFD-induced obesity, increasing the number of SCFA-producing bacteria (Bacteroidaceae) and reducing opportunistic pathogens (Enterobacteriaceae and Desulfovibrionaceae).
33151233	4	26	theme	hepatic	1034:1040	arg1	accumulation					1048:1059	hepatic lipid accumulation	1034:1059	hepatic lipid accumulation	1034:1059	Besides, MLDs could alleviate dyslipidaemia, significantly suppress hepatic lipid accumulation and steatosis and effectively ameliorate lipid metabolism.
33151233	0	27	theme	LPS	55:57	arg1	levels					59:64	serum LPS levels	49:64	serum LPS levels	49:64	Black garlic melanoidins prevent obesity, reduce serum LPS levels and modulate the gut microbiota composition in high-fat diet-induced obese C57BL/6J mice.
33151233	1	28	theme	animal	300:305	arg1	model					307:311	an animal model	297:311	an animal model	297:311	The objective of this study is to assess the potential anti-obesity effects of black garlic melanoidins (MLDs) and gut microbiota changes in an animal model, hypothesizing that the effects of oral administration of MLDs can be partially mediated by the modulation of intestinal microbiota via inhibiting the formation of lipopolysaccharides (LPS) and promoting the production of short-chain fatty acids (SCFAs).
33151233	7	29	theme	bacterial	1574:1582	arg1	diversity					1584:1592	bacterial diversity	1574:1592	bacterial diversity	1574:1592	Moreover, after the oral administration of MLDs, the intestinal microbial environment improved in the sense that bacterial diversity and richness increased.
33151233	6	30	theme	synthase	1267:1274	arg1	expression					1242:1251	the expression	1238:1251	the expression of fatty acid synthase (FAS) and interleukin-6 (IL-6)	1238:1305	The MLDs could down-regulate the expression of fatty acid synthase (FAS) and interleukin-6 (IL-6) and up-regulate the expression of adipose triacylglyceride lipase (ATGL) and hormone sensitive lipase (HSL) in adipose tissues and livers at mRNA levels.
33151233	2	31	theme	C57BL/6J	591:598	arg1	mice					600:603	C57BL/6J mice	591:603	C57BL/6J mice with high-fat diet (HFD)-induced obesity	591:644	The effects of MLDs in C57BL/6J mice with high-fat diet (HFD)-induced obesity were investigated for 12 weeks with low (50 mg kg-1 day-1), medium (100 mg kg-1 day-1) and high (200 mg kg-1 day-1) doses.
33151233	0	32	theme	microbiota	87:96	arg1	composition					98:108	the gut microbiota composition	79:108	the gut microbiota composition in high-fat diet-induced obese C57BL/6J mice	79:153	Black garlic melanoidins prevent obesity, reduce serum LPS levels and modulate the gut microbiota composition in high-fat diet-induced obese C57BL/6J mice.
33151233	4	33	theme	lipid	1102:1106	arg1	metabolism					1108:1117	lipid metabolism	1102:1117	lipid metabolism	1102:1117	Besides, MLDs could alleviate dyslipidaemia, significantly suppress hepatic lipid accumulation and steatosis and effectively ameliorate lipid metabolism.
33151233	0	34	theme	Black	0:4	arg1	melanoidins					13:23	Black garlic melanoidins	0:23	Black garlic melanoidins	0:23	Black garlic melanoidins prevent obesity, reduce serum LPS levels and modulate the gut microbiota composition in high-fat diet-induced obese C57BL/6J mice.
33151233	8	35	theme	SCFA-producing	1728:1741	arg1	Bacteroidaceae					1753:1766	Bacteroidaceae	1753:1766	Bacteroidaceae	1753:1766	Intervention with MLDs modified the gut microbiota in mice with HFD-induced obesity, increasing the number of SCFA-producing bacteria (Bacteroidaceae) and reducing opportunistic pathogens (Enterobacteriaceae and Desulfovibrionaceae).
33151233	8	35	theme	SCFA-producing	1728:1741	arg1	bacteria					1743:1750	SCFA-producing bacteria	1728:1750	SCFA-producing bacteria (Bacteroidaceae)	1728:1767	Intervention with MLDs modified the gut microbiota in mice with HFD-induced obesity, increasing the number of SCFA-producing bacteria (Bacteroidaceae) and reducing opportunistic pathogens (Enterobacteriaceae and Desulfovibrionaceae).
33151233	1	36	theme	fatty	547:551	arg1	SCFAs					560:564	SCFAs	560:564	SCFAs	560:564	The objective of this study is to assess the potential anti-obesity effects of black garlic melanoidins (MLDs) and gut microbiota changes in an animal model, hypothesizing that the effects of oral administration of MLDs can be partially mediated by the modulation of intestinal microbiota via inhibiting the formation of lipopolysaccharides (LPS) and promoting the production of short-chain fatty acids (SCFAs).
33151233	1	36	theme	fatty	547:551	arg1	acids					553:557	short-chain fatty acids	535:557	short-chain fatty acids (SCFAs)	535:565	The objective of this study is to assess the potential anti-obesity effects of black garlic melanoidins (MLDs) and gut microbiota changes in an animal model, hypothesizing that the effects of oral administration of MLDs can be partially mediated by the modulation of intestinal microbiota via inhibiting the formation of lipopolysaccharides (LPS) and promoting the production of short-chain fatty acids (SCFAs).
33151233	3	37	theme	oral	796:799	arg1	administration					801:814	oral administration	796:814	oral administration of MLDs	796:822	The results indicated that oral administration of MLDs markedly reduced high fat diet-induced weight gain and white adipose tissue weights and reversed glucose tolerance, especially at high doses.
33151233	7	38	theme	intestinal	1514:1523	arg1	environment					1535:1545	the intestinal microbial environment	1510:1545	the intestinal microbial environment	1510:1545	Moreover, after the oral administration of MLDs, the intestinal microbial environment improved in the sense that bacterial diversity and richness increased.
33151233	1	39	theme	microbiota	275:284	arg1	changes					286:292	gut microbiota changes	271:292	gut microbiota changes in an animal model	271:311	The objective of this study is to assess the potential anti-obesity effects of black garlic melanoidins (MLDs) and gut microbiota changes in an animal model, hypothesizing that the effects of oral administration of MLDs can be partially mediated by the modulation of intestinal microbiota via inhibiting the formation of lipopolysaccharides (LPS) and promoting the production of short-chain fatty acids (SCFAs).
33151233	2	40	from	effects	572:578	arg1	mice					600:603	C57BL/6J mice	591:603	C57BL/6J mice with high-fat diet (HFD)-induced obesity	591:644	The effects of MLDs in C57BL/6J mice with high-fat diet (HFD)-induced obesity were investigated for 12 weeks with low (50 mg kg-1 day-1), medium (100 mg kg-1 day-1) and high (200 mg kg-1 day-1) doses.
33151233	2	41	with	weeks	671:675	arg1	high					737:740	high	737:740	high	737:740	The effects of MLDs in C57BL/6J mice with high-fat diet (HFD)-induced obesity were investigated for 12 weeks with low (50 mg kg-1 day-1), medium (100 mg kg-1 day-1) and high (200 mg kg-1 day-1) doses.
33151233	2	41	with	weeks	671:675	arg1	mg					718:719	100 mg kg-1 day-1	714:730	100 mg kg-1 day-1	714:730	The effects of MLDs in C57BL/6J mice with high-fat diet (HFD)-induced obesity were investigated for 12 weeks with low (50 mg kg-1 day-1), medium (100 mg kg-1 day-1) and high (200 mg kg-1 day-1) doses.
33151233	2	41	with	weeks	671:675	arg1	mg					747:748	200 mg kg-1 day-1	743:759	200 mg kg-1 day-1	743:759	The effects of MLDs in C57BL/6J mice with high-fat diet (HFD)-induced obesity were investigated for 12 weeks with low (50 mg kg-1 day-1), medium (100 mg kg-1 day-1) and high (200 mg kg-1 day-1) doses.
33151233	2	41	with	weeks	671:675	arg1	low					682:684	low	682:684	low	682:684	The effects of MLDs in C57BL/6J mice with high-fat diet (HFD)-induced obesity were investigated for 12 weeks with low (50 mg kg-1 day-1), medium (100 mg kg-1 day-1) and high (200 mg kg-1 day-1) doses.
33151233	2	41	with	weeks	671:675	arg1	medium					706:711	medium	706:711	medium (100 mg kg-1 day-1)	706:731	The effects of MLDs in C57BL/6J mice with high-fat diet (HFD)-induced obesity were investigated for 12 weeks with low (50 mg kg-1 day-1), medium (100 mg kg-1 day-1) and high (200 mg kg-1 day-1) doses.
33151233	2	41	with	weeks	671:675	arg1	mg					690:691	50 mg kg-1 day-1	687:702	50 mg kg-1 day-1	687:702	The effects of MLDs in C57BL/6J mice with high-fat diet (HFD)-induced obesity were investigated for 12 weeks with low (50 mg kg-1 day-1), medium (100 mg kg-1 day-1) and high (200 mg kg-1 day-1) doses.
33151233	3	42	theme	MLDs	819:822	arg1	administration					801:814	oral administration	796:814	oral administration of MLDs	796:822	The results indicated that oral administration of MLDs markedly reduced high fat diet-induced weight gain and white adipose tissue weights and reversed glucose tolerance, especially at high doses.
33151233	2	43	from	MLDs	583:586	arg1	mice					600:603	C57BL/6J mice	591:603	C57BL/6J mice with high-fat diet (HFD)-induced obesity	591:644	The effects of MLDs in C57BL/6J mice with high-fat diet (HFD)-induced obesity were investigated for 12 weeks with low (50 mg kg-1 day-1), medium (100 mg kg-1 day-1) and high (200 mg kg-1 day-1) doses.
33151233	1	44	theme	lipopolysaccharides	477:495	arg1	formation					464:472	the formation	460:472	the formation of lipopolysaccharides (LPS)	460:501	The objective of this study is to assess the potential anti-obesity effects of black garlic melanoidins (MLDs) and gut microbiota changes in an animal model, hypothesizing that the effects of oral administration of MLDs can be partially mediated by the modulation of intestinal microbiota via inhibiting the formation of lipopolysaccharides (LPS) and promoting the production of short-chain fatty acids (SCFAs).
33151233	1	45	theme	potential	201:209	arg1	effects					224:230	the potential anti-obesity effects	197:230	the potential anti-obesity effects of black garlic melanoidins (MLDs)	197:265	The objective of this study is to assess the potential anti-obesity effects of black garlic melanoidins (MLDs) and gut microbiota changes in an animal model, hypothesizing that the effects of oral administration of MLDs can be partially mediated by the modulation of intestinal microbiota via inhibiting the formation of lipopolysaccharides (LPS) and promoting the production of short-chain fatty acids (SCFAs).
33151233	3	46	theme	weight	863:868	arg1	gain					870:873	high fat diet-induced weight gain	841:873	high fat diet-induced weight gain	841:873	The results indicated that oral administration of MLDs markedly reduced high fat diet-induced weight gain and white adipose tissue weights and reversed glucose tolerance, especially at high doses.
33151233	11	47	used	used	2217:2220	arg2	MLDs					2170:2173	MLDs	2170:2173	MLDs	2170:2173	Therefore, it seems that MLDs exhibit anti-obesity effects and might be used as potential agents against obesity.
33151233	11	47	used	used	2217:2220	arg2	agents					2235:2240	potential agents	2225:2240	potential agents against obesity	2225:2256	Therefore, it seems that MLDs exhibit anti-obesity effects and might be used as potential agents against obesity.
33151233	3	48	theme	fat	846:848	arg1	gain					870:873	high fat diet-induced weight gain	841:873	high fat diet-induced weight gain	841:873	The results indicated that oral administration of MLDs markedly reduced high fat diet-induced weight gain and white adipose tissue weights and reversed glucose tolerance, especially at high doses.
33151233	6	49	theme	lipase	1366:1371	arg1	expression					1327:1336	the expression	1323:1336	the expression of adipose triacylglyceride lipase (ATGL) and hormone sensitive lipase (HSL) in adipose tissues and livers at mRNA levels	1323:1458	The MLDs could down-regulate the expression of fatty acid synthase (FAS) and interleukin-6 (IL-6) and up-regulate the expression of adipose triacylglyceride lipase (ATGL) and hormone sensitive lipase (HSL) in adipose tissues and livers at mRNA levels.
33151233	1	50	theme	black	235:239	arg1	MLDs					261:264	MLDs	261:264	MLDs	261:264	The objective of this study is to assess the potential anti-obesity effects of black garlic melanoidins (MLDs) and gut microbiota changes in an animal model, hypothesizing that the effects of oral administration of MLDs can be partially mediated by the modulation of intestinal microbiota via inhibiting the formation of lipopolysaccharides (LPS) and promoting the production of short-chain fatty acids (SCFAs).
33151233	1	50	theme	black	235:239	arg1	melanoidins					248:258	black garlic melanoidins	235:258	black garlic melanoidins (MLDs)	235:265	The objective of this study is to assess the potential anti-obesity effects of black garlic melanoidins (MLDs) and gut microbiota changes in an animal model, hypothesizing that the effects of oral administration of MLDs can be partially mediated by the modulation of intestinal microbiota via inhibiting the formation of lipopolysaccharides (LPS) and promoting the production of short-chain fatty acids (SCFAs).
33151233	3	51	theme	adipose	885:891	arg1	tissue					893:898	white adipose tissue	879:898	white adipose tissue	879:898	The results indicated that oral administration of MLDs markedly reduced high fat diet-induced weight gain and white adipose tissue weights and reversed glucose tolerance, especially at high doses.
33151233	8	52	dep	modified	1641:1648	arg1	reducing					1773:1780	reducing	1773:1780	reducing opportunistic pathogens (Enterobacteriaceae and Desulfovibrionaceae)	1773:1849	Intervention with MLDs modified the gut microbiota in mice with HFD-induced obesity, increasing the number of SCFA-producing bacteria (Bacteroidaceae) and reducing opportunistic pathogens (Enterobacteriaceae and Desulfovibrionaceae).
33151233	8	52	dep	modified	1641:1648	arg1	increasing					1703:1712	increasing	1703:1712	increasing the number of SCFA-producing bacteria (Bacteroidaceae)	1703:1767	Intervention with MLDs modified the gut microbiota in mice with HFD-induced obesity, increasing the number of SCFA-producing bacteria (Bacteroidaceae) and reducing opportunistic pathogens (Enterobacteriaceae and Desulfovibrionaceae).
33151233	6	53	theme	adipose	1341:1347	arg1	lipase					1366:1371	adipose triacylglyceride lipase	1341:1371	adipose triacylglyceride lipase (ATGL)	1341:1378	The MLDs could down-regulate the expression of fatty acid synthase (FAS) and interleukin-6 (IL-6) and up-regulate the expression of adipose triacylglyceride lipase (ATGL) and hormone sensitive lipase (HSL) in adipose tissues and livers at mRNA levels.
33151233	6	53	theme	adipose	1341:1347	arg1	ATGL					1374:1377	ATGL	1374:1377	ATGL	1374:1377	The MLDs could down-regulate the expression of fatty acid synthase (FAS) and interleukin-6 (IL-6) and up-regulate the expression of adipose triacylglyceride lipase (ATGL) and hormone sensitive lipase (HSL) in adipose tissues and livers at mRNA levels.
33151233	1	54	theme	melanoidins	248:258	arg1	effects					224:230	the potential anti-obesity effects	197:230	the potential anti-obesity effects of black garlic melanoidins (MLDs)	197:265	The objective of this study is to assess the potential anti-obesity effects of black garlic melanoidins (MLDs) and gut microbiota changes in an animal model, hypothesizing that the effects of oral administration of MLDs can be partially mediated by the modulation of intestinal microbiota via inhibiting the formation of lipopolysaccharides (LPS) and promoting the production of short-chain fatty acids (SCFAs).
33151233	1	54	theme	melanoidins	248:258	arg1	changes					286:292	gut microbiota changes	271:292	gut microbiota changes in an animal model	271:311	The objective of this study is to assess the potential anti-obesity effects of black garlic melanoidins (MLDs) and gut microbiota changes in an animal model, hypothesizing that the effects of oral administration of MLDs can be partially mediated by the modulation of intestinal microbiota via inhibiting the formation of lipopolysaccharides (LPS) and promoting the production of short-chain fatty acids (SCFAs).
33151233	11	55	theme	anti-obesity	2183:2194	arg1	effects					2196:2202	anti-obesity effects	2183:2202	anti-obesity effects	2183:2202	Therefore, it seems that MLDs exhibit anti-obesity effects and might be used as potential agents against obesity.
33151233	2	56	theme	kg-1	750:753	arg1	day-1					755:759	kg-1 day-1	750:759	200 mg kg-1 day-1	743:759	The effects of MLDs in C57BL/6J mice with high-fat diet (HFD)-induced obesity were investigated for 12 weeks with low (50 mg kg-1 day-1), medium (100 mg kg-1 day-1) and high (200 mg kg-1 day-1) doses.
33151233	6	57	theme	adipose	1418:1424	arg1	tissues					1426:1432	adipose tissues	1418:1432	adipose tissues	1418:1432	The MLDs could down-regulate the expression of fatty acid synthase (FAS) and interleukin-6 (IL-6) and up-regulate the expression of adipose triacylglyceride lipase (ATGL) and hormone sensitive lipase (HSL) in adipose tissues and livers at mRNA levels.
33151233	2	58	theme	MLDs	583:586	arg1	effects					572:578	The effects	568:578	The effects of MLDs in C57BL/6J mice with high-fat diet (HFD)-induced obesity	568:644	The effects of MLDs in C57BL/6J mice with high-fat diet (HFD)-induced obesity were investigated for 12 weeks with low (50 mg kg-1 day-1), medium (100 mg kg-1 day-1) and high (200 mg kg-1 day-1) doses.
33151233	10	59	theme	SCFAs	2039:2043	arg1	production					2025:2034	the production	2021:2034	the production of SCFAs	2021:2043	In conclusion, MLDs could improve glucose tolerance, induce the production of SCFAs and inhibit the production of endotoxin LPS, most likely mediated by modulating the gut microbiota.
33151233	1	60	theme	microbiota	434:443	arg1	modulation					409:418	the modulation	405:418	the modulation of intestinal microbiota	405:443	The objective of this study is to assess the potential anti-obesity effects of black garlic melanoidins (MLDs) and gut microbiota changes in an animal model, hypothesizing that the effects of oral administration of MLDs can be partially mediated by the modulation of intestinal microbiota via inhibiting the formation of lipopolysaccharides (LPS) and promoting the production of short-chain fatty acids (SCFAs).
33151233	2	61	dep	mg	718:719	arg1	day-1					726:730	kg-1 day-1	721:730	100 mg kg-1 day-1	714:730	The effects of MLDs in C57BL/6J mice with high-fat diet (HFD)-induced obesity were investigated for 12 weeks with low (50 mg kg-1 day-1), medium (100 mg kg-1 day-1) and high (200 mg kg-1 day-1) doses.
33151233	10	62	theme	endotoxin	2075:2083	arg1	LPS					2085:2087	endotoxin LPS	2075:2087	endotoxin LPS	2075:2087	In conclusion, MLDs could improve glucose tolerance, induce the production of SCFAs and inhibit the production of endotoxin LPS, most likely mediated by modulating the gut microbiota.
33151233	0	63	theme	high-fat	113:120	arg1	mice					150:153	high-fat diet-induced obese C57BL/6J mice	113:153	high-fat diet-induced obese C57BL/6J mice	113:153	Black garlic melanoidins prevent obesity, reduce serum LPS levels and modulate the gut microbiota composition in high-fat diet-induced obese C57BL/6J mice.
33151233	2	64	with	mice	600:603	arg1	obesity					638:644	high-fat diet (HFD)-induced obesity	610:644	high-fat diet (HFD)-induced obesity	610:644	The effects of MLDs in C57BL/6J mice with high-fat diet (HFD)-induced obesity were investigated for 12 weeks with low (50 mg kg-1 day-1), medium (100 mg kg-1 day-1) and high (200 mg kg-1 day-1) doses.
33151233	0	65	theme	obese	135:139	arg1	mice					150:153	high-fat diet-induced obese C57BL/6J mice	113:153	high-fat diet-induced obese C57BL/6J mice	113:153	Black garlic melanoidins prevent obesity, reduce serum LPS levels and modulate the gut microbiota composition in high-fat diet-induced obese C57BL/6J mice.
33151233	1	66	theme	administration	353:366	arg1	effects					337:343	the effects	333:343	the effects of oral administration of MLDs	333:374	The objective of this study is to assess the potential anti-obesity effects of black garlic melanoidins (MLDs) and gut microbiota changes in an animal model, hypothesizing that the effects of oral administration of MLDs can be partially mediated by the modulation of intestinal microbiota via inhibiting the formation of lipopolysaccharides (LPS) and promoting the production of short-chain fatty acids (SCFAs).
33151233	6	67	theme	sensitive	1392:1400	arg1	HSL					1410:1412	HSL	1410:1412	HSL	1410:1412	The MLDs could down-regulate the expression of fatty acid synthase (FAS) and interleukin-6 (IL-6) and up-regulate the expression of adipose triacylglyceride lipase (ATGL) and hormone sensitive lipase (HSL) in adipose tissues and livers at mRNA levels.
33151233	6	67	theme	sensitive	1392:1400	arg1	lipase					1402:1407	hormone sensitive lipase	1384:1407	hormone sensitive lipase (HSL)	1384:1413	The MLDs could down-regulate the expression of fatty acid synthase (FAS) and interleukin-6 (IL-6) and up-regulate the expression of adipose triacylglyceride lipase (ATGL) and hormone sensitive lipase (HSL) in adipose tissues and livers at mRNA levels.
33151233	7	68	theme	oral	1481:1484	arg1	administration					1486:1499	the oral administration	1477:1499	the oral administration of MLDs	1477:1507	Moreover, after the oral administration of MLDs, the intestinal microbial environment improved in the sense that bacterial diversity and richness increased.
33151233	8	69	from	microbiota	1658:1667	arg1	mice					1672:1675	mice	1672:1675	mice with HFD-induced obesity	1672:1700	Intervention with MLDs modified the gut microbiota in mice with HFD-induced obesity, increasing the number of SCFA-producing bacteria (Bacteroidaceae) and reducing opportunistic pathogens (Enterobacteriaceae and Desulfovibrionaceae).
33151233	1	70	from	effects	224:230	arg1	model					307:311	an animal model	297:311	an animal model	297:311	The objective of this study is to assess the potential anti-obesity effects of black garlic melanoidins (MLDs) and gut microbiota changes in an animal model, hypothesizing that the effects of oral administration of MLDs can be partially mediated by the modulation of intestinal microbiota via inhibiting the formation of lipopolysaccharides (LPS) and promoting the production of short-chain fatty acids (SCFAs).
33151233	6	71	from	expression	1327:1336	arg1	livers					1438:1443	livers	1438:1443	livers at mRNA levels	1438:1458	The MLDs could down-regulate the expression of fatty acid synthase (FAS) and interleukin-6 (IL-6) and up-regulate the expression of adipose triacylglyceride lipase (ATGL) and hormone sensitive lipase (HSL) in adipose tissues and livers at mRNA levels.
33151233	6	71	from	expression	1327:1336	arg1	levels					1453:1458	mRNA levels	1448:1458	mRNA levels	1448:1458	The MLDs could down-regulate the expression of fatty acid synthase (FAS) and interleukin-6 (IL-6) and up-regulate the expression of adipose triacylglyceride lipase (ATGL) and hormone sensitive lipase (HSL) in adipose tissues and livers at mRNA levels.
33151233	6	71	from	expression	1327:1336	arg1	tissues					1426:1432	adipose tissues	1418:1432	adipose tissues	1418:1432	The MLDs could down-regulate the expression of fatty acid synthase (FAS) and interleukin-6 (IL-6) and up-regulate the expression of adipose triacylglyceride lipase (ATGL) and hormone sensitive lipase (HSL) in adipose tissues and livers at mRNA levels.
33151233	0	72	from	composition	98:108	arg1	mice					150:153	high-fat diet-induced obese C57BL/6J mice	113:153	high-fat diet-induced obese C57BL/6J mice	113:153	Black garlic melanoidins prevent obesity, reduce serum LPS levels and modulate the gut microbiota composition in high-fat diet-induced obese C57BL/6J mice.
33151233	9	73	theme	other	1878:1882	arg1	probiotics					1884:1893	other probiotics	1878:1893	other probiotics	1878:1893	An increased abundance of other probiotics including Lactobacillaceae and Akkermansiaceae was also observed.
33151233	9	74	theme	increased	1855:1863	arg1	abundance					1865:1873	An increased abundance	1852:1873	An increased abundance of other probiotics including Lactobacillaceae and Akkermansiaceae	1852:1940	An increased abundance of other probiotics including Lactobacillaceae and Akkermansiaceae was also observed.
33151233	10	75	theme	LPS	2085:2087	arg1	production					2061:2070	the production	2057:2070	the production	2057:2070	In conclusion, MLDs could improve glucose tolerance, induce the production of SCFAs and inhibit the production of endotoxin LPS, most likely mediated by modulating the gut microbiota.
33151233	4	76	theme	lipid	1042:1046	arg1	accumulation					1048:1059	hepatic lipid accumulation	1034:1059	hepatic lipid accumulation	1034:1059	Besides, MLDs could alleviate dyslipidaemia, significantly suppress hepatic lipid accumulation and steatosis and effectively ameliorate lipid metabolism.
33151233	5	77	theme	dose-dependent	1186:1199	arg1	manner					1201:1206	a dose-dependent manner	1184:1206	a dose-dependent manner	1184:1206	The plasma LPS reduced significantly and the SCFAs increased in a dose-dependent manner.
33151233	6	78	theme	interleukin-6	1286:1298	arg1	expression					1242:1251	the expression	1238:1251	the expression of fatty acid synthase (FAS) and interleukin-6 (IL-6)	1238:1305	The MLDs could down-regulate the expression of fatty acid synthase (FAS) and interleukin-6 (IL-6) and up-regulate the expression of adipose triacylglyceride lipase (ATGL) and hormone sensitive lipase (HSL) in adipose tissues and livers at mRNA levels.
33151233	10	79	theme	glucose	1995:2001	arg1	tolerance					2003:2011	glucose tolerance	1995:2011	glucose tolerance	1995:2011	In conclusion, MLDs could improve glucose tolerance, induce the production of SCFAs and inhibit the production of endotoxin LPS, most likely mediated by modulating the gut microbiota.
33151233	5	80	theme	plasma	1124:1129	arg1	LPS					1131:1133	The plasma LPS	1120:1133	The plasma LPS	1120:1133	The plasma LPS reduced significantly and the SCFAs increased in a dose-dependent manner.
33151233	2	81	theme	high-fat	610:617	arg1	HFD					625:627	HFD	625:627	HFD	625:627	The effects of MLDs in C57BL/6J mice with high-fat diet (HFD)-induced obesity were investigated for 12 weeks with low (50 mg kg-1 day-1), medium (100 mg kg-1 day-1) and high (200 mg kg-1 day-1) doses.
33151233	2	81	theme	high-fat	610:617	arg1	diet					619:622	high-fat diet	610:622	high-fat diet (HFD)	610:628	The effects of MLDs in C57BL/6J mice with high-fat diet (HFD)-induced obesity were investigated for 12 weeks with low (50 mg kg-1 day-1), medium (100 mg kg-1 day-1) and high (200 mg kg-1 day-1) doses.
33151233	0	82	theme	gut	83:85	arg1	composition					98:108	the gut microbiota composition	79:108	the gut microbiota composition in high-fat diet-induced obese C57BL/6J mice	79:153	Black garlic melanoidins prevent obesity, reduce serum LPS levels and modulate the gut microbiota composition in high-fat diet-induced obese C57BL/6J mice.
33151233	8	83	theme	HFD-induced	1682:1692	arg1	obesity					1694:1700	HFD-induced obesity	1682:1700	HFD-induced obesity	1682:1700	Intervention with MLDs modified the gut microbiota in mice with HFD-induced obesity, increasing the number of SCFA-producing bacteria (Bacteroidaceae) and reducing opportunistic pathogens (Enterobacteriaceae and Desulfovibrionaceae).
33151233	6	84	theme	acid	1262:1265	arg1	synthase					1267:1274	fatty acid synthase	1256:1274	fatty acid synthase (FAS)	1256:1280	The MLDs could down-regulate the expression of fatty acid synthase (FAS) and interleukin-6 (IL-6) and up-regulate the expression of adipose triacylglyceride lipase (ATGL) and hormone sensitive lipase (HSL) in adipose tissues and livers at mRNA levels.
33151233	6	84	theme	acid	1262:1265	arg1	FAS					1277:1279	FAS	1277:1279	FAS	1277:1279	The MLDs could down-regulate the expression of fatty acid synthase (FAS) and interleukin-6 (IL-6) and up-regulate the expression of adipose triacylglyceride lipase (ATGL) and hormone sensitive lipase (HSL) in adipose tissues and livers at mRNA levels.
33151233	8	85	with	Intervention	1618:1629	arg1	MLDs					1636:1639	MLDs	1636:1639	MLDs	1636:1639	Intervention with MLDs modified the gut microbiota in mice with HFD-induced obesity, increasing the number of SCFA-producing bacteria (Bacteroidaceae) and reducing opportunistic pathogens (Enterobacteriaceae and Desulfovibrionaceae).
33151233	0	86	theme	garlic	6:11	arg1	melanoidins					13:23	Black garlic melanoidins	0:23	Black garlic melanoidins	0:23	Black garlic melanoidins prevent obesity, reduce serum LPS levels and modulate the gut microbiota composition in high-fat diet-induced obese C57BL/6J mice.
33151233	3	87	theme	high	954:957	arg1	doses					959:963	high doses	954:963	high doses	954:963	The results indicated that oral administration of MLDs markedly reduced high fat diet-induced weight gain and white adipose tissue weights and reversed glucose tolerance, especially at high doses.
33151233	1	88	theme	short-chain	535:545	arg1	SCFAs					560:564	SCFAs	560:564	SCFAs	560:564	The objective of this study is to assess the potential anti-obesity effects of black garlic melanoidins (MLDs) and gut microbiota changes in an animal model, hypothesizing that the effects of oral administration of MLDs can be partially mediated by the modulation of intestinal microbiota via inhibiting the formation of lipopolysaccharides (LPS) and promoting the production of short-chain fatty acids (SCFAs).
33151233	1	88	theme	short-chain	535:545	arg1	acids					553:557	short-chain fatty acids	535:557	short-chain fatty acids (SCFAs)	535:565	The objective of this study is to assess the potential anti-obesity effects of black garlic melanoidins (MLDs) and gut microbiota changes in an animal model, hypothesizing that the effects of oral administration of MLDs can be partially mediated by the modulation of intestinal microbiota via inhibiting the formation of lipopolysaccharides (LPS) and promoting the production of short-chain fatty acids (SCFAs).
33151233	10	89	theme	gut	2129:2131	arg1	microbiota					2133:2142	the gut microbiota	2125:2142	the gut microbiota	2125:2142	In conclusion, MLDs could improve glucose tolerance, induce the production of SCFAs and inhibit the production of endotoxin LPS, most likely mediated by modulating the gut microbiota.
33151233	3	90	dep	gain	870:873	arg1	weights					900:906	weights	900:906	weights	900:906	The results indicated that oral administration of MLDs markedly reduced high fat diet-induced weight gain and white adipose tissue weights and reversed glucose tolerance, especially at high doses.
33151233	1	91	theme	acids	553:557	arg1	production					521:530	the production	517:530	the production of short-chain fatty acids (SCFAs)	517:565	The objective of this study is to assess the potential anti-obesity effects of black garlic melanoidins (MLDs) and gut microbiota changes in an animal model, hypothesizing that the effects of oral administration of MLDs can be partially mediated by the modulation of intestinal microbiota via inhibiting the formation of lipopolysaccharides (LPS) and promoting the production of short-chain fatty acids (SCFAs).
33151233	1	92	theme	gut	271:273	arg1	changes					286:292	gut microbiota changes	271:292	gut microbiota changes in an animal model	271:311	The objective of this study is to assess the potential anti-obesity effects of black garlic melanoidins (MLDs) and gut microbiota changes in an animal model, hypothesizing that the effects of oral administration of MLDs can be partially mediated by the modulation of intestinal microbiota via inhibiting the formation of lipopolysaccharides (LPS) and promoting the production of short-chain fatty acids (SCFAs).
33151233	7	93	theme	microbial	1525:1533	arg1	environment					1535:1545	the intestinal microbial environment	1510:1545	the intestinal microbial environment	1510:1545	Moreover, after the oral administration of MLDs, the intestinal microbial environment improved in the sense that bacterial diversity and richness increased.
33151233	6	94	theme	fatty	1256:1260	arg1	synthase					1267:1274	fatty acid synthase	1256:1274	fatty acid synthase (FAS)	1256:1280	The MLDs could down-regulate the expression of fatty acid synthase (FAS) and interleukin-6 (IL-6) and up-regulate the expression of adipose triacylglyceride lipase (ATGL) and hormone sensitive lipase (HSL) in adipose tissues and livers at mRNA levels.
33151233	6	94	theme	fatty	1256:1260	arg1	FAS					1277:1279	FAS	1277:1279	FAS	1277:1279	The MLDs could down-regulate the expression of fatty acid synthase (FAS) and interleukin-6 (IL-6) and up-regulate the expression of adipose triacylglyceride lipase (ATGL) and hormone sensitive lipase (HSL) in adipose tissues and livers at mRNA levels.
33151233	0	95	theme	serum	49:53	arg1	levels					59:64	serum LPS levels	49:64	serum LPS levels	49:64	Black garlic melanoidins prevent obesity, reduce serum LPS levels and modulate the gut microbiota composition in high-fat diet-induced obese C57BL/6J mice.
33151233	3	96	theme	high	841:844	arg1	gain					870:873	high fat diet-induced weight gain	841:873	high fat diet-induced weight gain	841:873	The results indicated that oral administration of MLDs markedly reduced high fat diet-induced weight gain and white adipose tissue weights and reversed glucose tolerance, especially at high doses.
33151233	6	97	theme	hormone	1384:1390	arg1	HSL					1410:1412	HSL	1410:1412	HSL	1410:1412	The MLDs could down-regulate the expression of fatty acid synthase (FAS) and interleukin-6 (IL-6) and up-regulate the expression of adipose triacylglyceride lipase (ATGL) and hormone sensitive lipase (HSL) in adipose tissues and livers at mRNA levels.
33151233	6	97	theme	hormone	1384:1390	arg1	lipase					1402:1407	hormone sensitive lipase	1384:1407	hormone sensitive lipase (HSL)	1384:1413	The MLDs could down-regulate the expression of fatty acid synthase (FAS) and interleukin-6 (IL-6) and up-regulate the expression of adipose triacylglyceride lipase (ATGL) and hormone sensitive lipase (HSL) in adipose tissues and livers at mRNA levels.
33151233	2	98	dep	mg	690:691	arg1	day-1					698:702	kg-1 day-1	693:702	50 mg kg-1 day-1	687:702	The effects of MLDs in C57BL/6J mice with high-fat diet (HFD)-induced obesity were investigated for 12 weeks with low (50 mg kg-1 day-1), medium (100 mg kg-1 day-1) and high (200 mg kg-1 day-1) doses.
33151233	2	99	dep	mg	747:748	arg1	day-1					755:759	kg-1 day-1	750:759	200 mg kg-1 day-1	743:759	The effects of MLDs in C57BL/6J mice with high-fat diet (HFD)-induced obesity were investigated for 12 weeks with low (50 mg kg-1 day-1), medium (100 mg kg-1 day-1) and high (200 mg kg-1 day-1) doses.
33151233	1	100	theme	anti-obesity	211:222	arg1	effects					224:230	the potential anti-obesity effects	197:230	the potential anti-obesity effects of black garlic melanoidins (MLDs)	197:265	The objective of this study is to assess the potential anti-obesity effects of black garlic melanoidins (MLDs) and gut microbiota changes in an animal model, hypothesizing that the effects of oral administration of MLDs can be partially mediated by the modulation of intestinal microbiota via inhibiting the formation of lipopolysaccharides (LPS) and promoting the production of short-chain fatty acids (SCFAs).
33151233	2	101	dep	low	682:684	arg1	doses					762:766	doses	762:766	doses	762:766	The effects of MLDs in C57BL/6J mice with high-fat diet (HFD)-induced obesity were investigated for 12 weeks with low (50 mg kg-1 day-1), medium (100 mg kg-1 day-1) and high (200 mg kg-1 day-1) doses.
33151233	11	102	theme	potential	2225:2233	arg1	agents					2235:2240	potential agents	2225:2240	potential agents against obesity	2225:2256	Therefore, it seems that MLDs exhibit anti-obesity effects and might be used as potential agents against obesity.
33151233	11	102	theme	potential	2225:2233	arg1	MLDs					2170:2173	MLDs	2170:2173	MLDs	2170:2173	Therefore, it seems that MLDs exhibit anti-obesity effects and might be used as potential agents against obesity.
32531008	8	0	theme	hemostatic	1465:1474	arg1	potential					1450:1458	promising potential	1440:1458	promising potential	1440:1458	This PACG sponge holds promising potential as a hemostatic agent applicable in an emergency.
32531008	8	0	theme	hemostatic	1465:1474	arg1	agent					1476:1480	a hemostatic agent	1463:1480	a hemostatic agent applicable in an emergency	1463:1507	This PACG sponge holds promising potential as a hemostatic agent applicable in an emergency.
32531008	5	1	with	generation	852:861	arg1	strengths					907:915	robust compressive strengths	888:915	robust compressive strengths due to the hydrogen bonding interactions of PACG	888:964	Further lyophilization of the foaming hydrogels leads to the final generation of PACG/CMC sponges with robust compressive strengths due to the hydrogen bonding interactions of PACG.
32531008	3	2	theme	hydrogen	469:476	arg1	monomer					513:519	a hydrogen bonding N-acryloyl-2-glycine (ACG) monomer	467:519	a hydrogen bonding N-acryloyl-2-glycine (ACG) monomer	467:519	A thickening agent, carboxymethyl cellulose (CMC), is incorporated into a hydrogen bonding N-acryloyl-2-glycine (ACG) monomer and an initiator, and vortexing generates air bubbles in the viscous liquid.
32531008	6	3	theme	PACG/CMC	967:974	arg1	sponges					976:982	PACG/CMC sponges	967:982	PACG/CMC sponges	967:982	PACG/CMC sponges are shown to demonstrate a tunable liquid absorption ability, in vitro hemostatic ability, better hemocompatibility and cytocompatibility.
32531008	3	4	theme	bonding	478:484	arg1	monomer					513:519	a hydrogen bonding N-acryloyl-2-glycine (ACG) monomer	467:519	a hydrogen bonding N-acryloyl-2-glycine (ACG) monomer	467:519	A thickening agent, carboxymethyl cellulose (CMC), is incorporated into a hydrogen bonding N-acryloyl-2-glycine (ACG) monomer and an initiator, and vortexing generates air bubbles in the viscous liquid.
32531008	8	5	theme	applicable	1482:1491	arg1	potential					1450:1458	promising potential	1440:1458	promising potential	1440:1458	This PACG sponge holds promising potential as a hemostatic agent applicable in an emergency.
32531008	8	5	theme	applicable	1482:1491	arg1	agent					1476:1480	a hemostatic agent	1463:1480	a hemostatic agent applicable in an emergency	1463:1507	This PACG sponge holds promising potential as a hemostatic agent applicable in an emergency.
32531008	7	6	theme	bleeding	1235:1242	arg1	time					1244:1247	the bleeding time	1231:1247	the bleeding time	1231:1247	In a rat liver injury model and a femoral artery injury model, the PACG/CMC sponge can significantly reduce the bleeding time and blood loss compared with gauze and commercial gelatin sponge because of the high blood absorption ability and effective concentration of blood coagulation factors.
32531008	6	7	theme	hemostatic	1055:1064	arg1	ability					1066:1072	in vitro hemostatic ability	1046:1072	in vitro hemostatic ability	1046:1072	PACG/CMC sponges are shown to demonstrate a tunable liquid absorption ability, in vitro hemostatic ability, better hemocompatibility and cytocompatibility.
32531008	7	8	theme	commercial	1288:1297	arg1	sponge					1307:1312	commercial gelatin sponge	1288:1312	commercial gelatin sponge	1288:1312	In a rat liver injury model and a femoral artery injury model, the PACG/CMC sponge can significantly reduce the bleeding time and blood loss compared with gauze and commercial gelatin sponge because of the high blood absorption ability and effective concentration of blood coagulation factors.
32531008	2	9	dep	in	324:325	arg1	situ					327:330	situ	327:330	situ	327:330	In this work, we propose the fabrication of a robust hemostatic sponge by a hydrogen bond strengthening and in situ bubble expanding strategy in thermo-initiation polymerization.
32531008	4	10	from	aids	651:654	arg1	expanding					659:667	expanding	659:667	expanding	659:667	Heating initiates fast polymerization, and meanwhile aids in expanding of bubbles, which results in the fixation of bubbles throughout the network, and the formation of porous hydrogels.
32531008	7	11	theme	injury	1172:1177	arg1	model					1179:1183	a femoral artery injury model	1155:1183	a femoral artery injury model	1155:1183	In a rat liver injury model and a femoral artery injury model, the PACG/CMC sponge can significantly reduce the bleeding time and blood loss compared with gauze and commercial gelatin sponge because of the high blood absorption ability and effective concentration of blood coagulation factors.
32531008	5	12	theme	hydrogen	928:935	arg1	interactions					945:956	the hydrogen bonding interactions	924:956	the hydrogen bonding interactions of PACG	924:964	Further lyophilization of the foaming hydrogels leads to the final generation of PACG/CMC sponges with robust compressive strengths due to the hydrogen bonding interactions of PACG.
32531008	4	13	theme	bubbles	714:720	arg1	fixation					702:709	the fixation	698:709	the fixation of bubbles throughout the network	698:743	Heating initiates fast polymerization, and meanwhile aids in expanding of bubbles, which results in the fixation of bubbles throughout the network, and the formation of porous hydrogels.
32531008	5	14	theme	foaming	815:821	arg1	hydrogels					823:831	the foaming hydrogels	811:831	the foaming hydrogels	811:831	Further lyophilization of the foaming hydrogels leads to the final generation of PACG/CMC sponges with robust compressive strengths due to the hydrogen bonding interactions of PACG.
32531008	5	15	theme	PACG/CMC	866:873	arg1	sponges					875:881	PACG/CMC sponges	866:881	PACG/CMC sponges	866:881	Further lyophilization of the foaming hydrogels leads to the final generation of PACG/CMC sponges with robust compressive strengths due to the hydrogen bonding interactions of PACG.
32531008	6	16	theme	tunable	1011:1017	arg1	ability					1037:1043	a tunable liquid absorption ability	1009:1043	a tunable liquid absorption ability	1009:1043	PACG/CMC sponges are shown to demonstrate a tunable liquid absorption ability, in vitro hemostatic ability, better hemocompatibility and cytocompatibility.
32531008	1	17	used	used	122:125	arg2	sponge					103:108	a hemostatic sponge	90:108	a hemostatic sponge that can be used for treating both arterial hemorrhage and non-compressible bleeding	90:193	The development of a hemostatic sponge that can be used for treating both arterial hemorrhage and non-compressible bleeding remains a challenge.
32531008	2	18	theme	thermo-initiation	361:377	arg1	polymerization					379:392	thermo-initiation polymerization	361:392	thermo-initiation polymerization	361:392	In this work, we propose the fabrication of a robust hemostatic sponge by a hydrogen bond strengthening and in situ bubble expanding strategy in thermo-initiation polymerization.
32531008	7	19	theme	femoral	1157:1163	arg1	model					1179:1183	a femoral artery injury model	1155:1183	a femoral artery injury model	1155:1183	In a rat liver injury model and a femoral artery injury model, the PACG/CMC sponge can significantly reduce the bleeding time and blood loss compared with gauze and commercial gelatin sponge because of the high blood absorption ability and effective concentration of blood coagulation factors.
32531008	7	20	theme	coagulation	1396:1406	arg1	factors					1408:1414	blood coagulation factors	1390:1414	blood coagulation factors	1390:1414	In a rat liver injury model and a femoral artery injury model, the PACG/CMC sponge can significantly reduce the bleeding time and blood loss compared with gauze and commercial gelatin sponge because of the high blood absorption ability and effective concentration of blood coagulation factors.
32531008	1	21	theme	arterial	145:152	arg1	hemorrhage					154:163	arterial hemorrhage	145:163	arterial hemorrhage	145:163	The development of a hemostatic sponge that can be used for treating both arterial hemorrhage and non-compressible bleeding remains a challenge.
32531008	0	22	theme	robust	2:7	arg1	sponge					42:47	A robust poly(N-acryloyl-2-glycine)-based sponge	0:47	A robust poly(N-acryloyl-2-glycine)-based sponge for rapid hemostasis	0:68	A robust poly(N-acryloyl-2-glycine)-based sponge for rapid hemostasis.
32531008	6	23	theme	in	1046:1047	arg1	ability					1066:1072	in vitro hemostatic ability	1046:1072	in vitro hemostatic ability	1046:1072	PACG/CMC sponges are shown to demonstrate a tunable liquid absorption ability, in vitro hemostatic ability, better hemocompatibility and cytocompatibility.
32531008	5	24	theme	compressive	895:905	arg1	strengths					907:915	robust compressive strengths	888:915	robust compressive strengths due to the hydrogen bonding interactions of PACG	888:964	Further lyophilization of the foaming hydrogels leads to the final generation of PACG/CMC sponges with robust compressive strengths due to the hydrogen bonding interactions of PACG.
32531008	4	25	from	polymerization	621:634	arg1	expanding					659:667	expanding	659:667	expanding	659:667	Heating initiates fast polymerization, and meanwhile aids in expanding of bubbles, which results in the fixation of bubbles throughout the network, and the formation of porous hydrogels.
32531008	4	26	theme	meanwhile	641:649	arg1	aids					651:654	meanwhile aids	641:654	meanwhile aids	641:654	Heating initiates fast polymerization, and meanwhile aids in expanding of bubbles, which results in the fixation of bubbles throughout the network, and the formation of porous hydrogels.
32531008	8	27	from	applicable	1482:1491	arg1	emergency					1499:1507	an emergency	1496:1507	an emergency	1496:1507	This PACG sponge holds promising potential as a hemostatic agent applicable in an emergency.
32531008	7	28	theme	rat	1128:1130	arg1	model					1145:1149	a rat liver injury model	1126:1149	a rat liver injury model	1126:1149	In a rat liver injury model and a femoral artery injury model, the PACG/CMC sponge can significantly reduce the bleeding time and blood loss compared with gauze and commercial gelatin sponge because of the high blood absorption ability and effective concentration of blood coagulation factors.
32531008	2	29	theme	sponge	280:285	arg1	fabrication					245:255	the fabrication	241:255	the fabrication of a robust hemostatic sponge by a hydrogen bond strengthening and in situ bubble expanding strategy in thermo-initiation polymerization	241:392	In this work, we propose the fabrication of a robust hemostatic sponge by a hydrogen bond strengthening and in situ bubble expanding strategy in thermo-initiation polymerization.
32531008	2	30	theme	expanding	339:347	arg1	strategy					349:356	and in situ bubble expanding strategy	320:356	strategy	349:356	In this work, we propose the fabrication of a robust hemostatic sponge by a hydrogen bond strengthening and in situ bubble expanding strategy in thermo-initiation polymerization.
32531008	7	31	theme	liver	1132:1136	arg1	model					1145:1149	a rat liver injury model	1126:1149	a rat liver injury model	1126:1149	In a rat liver injury model and a femoral artery injury model, the PACG/CMC sponge can significantly reduce the bleeding time and blood loss compared with gauze and commercial gelatin sponge because of the high blood absorption ability and effective concentration of blood coagulation factors.
32531008	7	32	theme	blood	1334:1338	arg1	ability					1351:1357	the high blood absorption ability	1325:1357	the high blood absorption ability	1325:1357	In a rat liver injury model and a femoral artery injury model, the PACG/CMC sponge can significantly reduce the bleeding time and blood loss compared with gauze and commercial gelatin sponge because of the high blood absorption ability and effective concentration of blood coagulation factors.
32531008	2	33	theme	hemostatic	269:278	arg1	sponge					280:285	a robust hemostatic sponge	260:285	a robust hemostatic sponge	260:285	In this work, we propose the fabrication of a robust hemostatic sponge by a hydrogen bond strengthening and in situ bubble expanding strategy in thermo-initiation polymerization.
32531008	4	34	theme	porous	767:772	arg1	hydrogels					774:782	porous hydrogels	767:782	porous hydrogels	767:782	Heating initiates fast polymerization, and meanwhile aids in expanding of bubbles, which results in the fixation of bubbles throughout the network, and the formation of porous hydrogels.
32531008	5	35	theme	Further	785:791	arg1	lyophilization					793:806	Further lyophilization	785:806	Further lyophilization of the foaming hydrogels	785:831	Further lyophilization of the foaming hydrogels leads to the final generation of PACG/CMC sponges with robust compressive strengths due to the hydrogen bonding interactions of PACG.
32531008	6	36	theme	better	1075:1080	arg1	hemocompatibility					1082:1098	better hemocompatibility	1075:1098	better hemocompatibility	1075:1098	PACG/CMC sponges are shown to demonstrate a tunable liquid absorption ability, in vitro hemostatic ability, better hemocompatibility and cytocompatibility.
32531008	3	37	theme	ACG	508:510	arg1	monomer					513:519	a hydrogen bonding N-acryloyl-2-glycine (ACG) monomer	467:519	a hydrogen bonding N-acryloyl-2-glycine (ACG) monomer	467:519	A thickening agent, carboxymethyl cellulose (CMC), is incorporated into a hydrogen bonding N-acryloyl-2-glycine (ACG) monomer and an initiator, and vortexing generates air bubbles in the viscous liquid.
32531008	8	38	theme	PACG	1422:1425	arg1	sponge					1427:1432	This PACG sponge	1417:1432	This PACG sponge	1417:1432	This PACG sponge holds promising potential as a hemostatic agent applicable in an emergency.
32531008	2	39	theme	robust	262:267	arg1	sponge					280:285	a robust hemostatic sponge	260:285	a robust hemostatic sponge	260:285	In this work, we propose the fabrication of a robust hemostatic sponge by a hydrogen bond strengthening and in situ bubble expanding strategy in thermo-initiation polymerization.
32531008	2	40	theme	bubble	332:337	arg1	strategy					349:356	and in situ bubble expanding strategy	320:356	strategy	349:356	In this work, we propose the fabrication of a robust hemostatic sponge by a hydrogen bond strengthening and in situ bubble expanding strategy in thermo-initiation polymerization.
32531008	7	41	theme	artery	1165:1170	arg1	model					1179:1183	a femoral artery injury model	1155:1183	a femoral artery injury model	1155:1183	In a rat liver injury model and a femoral artery injury model, the PACG/CMC sponge can significantly reduce the bleeding time and blood loss compared with gauze and commercial gelatin sponge because of the high blood absorption ability and effective concentration of blood coagulation factors.
32531008	5	42	theme	sponges	875:881	arg1	generation					852:861	the final generation	842:861	the final generation of PACG/CMC sponges with robust compressive strengths due to the hydrogen bonding interactions of PACG	842:964	Further lyophilization of the foaming hydrogels leads to the final generation of PACG/CMC sponges with robust compressive strengths due to the hydrogen bonding interactions of PACG.
32531008	7	43	theme	blood	1253:1257	arg1	loss					1259:1262	blood loss	1253:1262	blood loss	1253:1262	In a rat liver injury model and a femoral artery injury model, the PACG/CMC sponge can significantly reduce the bleeding time and blood loss compared with gauze and commercial gelatin sponge because of the high blood absorption ability and effective concentration of blood coagulation factors.
32531008	3	44	theme	air	563:565	arg1	bubbles					567:573	air bubbles	563:573	air bubbles in the viscous liquid	563:595	A thickening agent, carboxymethyl cellulose (CMC), is incorporated into a hydrogen bonding N-acryloyl-2-glycine (ACG) monomer and an initiator, and vortexing generates air bubbles in the viscous liquid.
32531008	3	45	from	bubbles	567:573	arg1	liquid					590:595	liquid	590:595	liquid	590:595	A thickening agent, carboxymethyl cellulose (CMC), is incorporated into a hydrogen bonding N-acryloyl-2-glycine (ACG) monomer and an initiator, and vortexing generates air bubbles in the viscous liquid.
32531008	4	46	theme	fast	616:619	arg1	polymerization					621:634	fast polymerization	616:634	fast polymerization	616:634	Heating initiates fast polymerization, and meanwhile aids in expanding of bubbles, which results in the fixation of bubbles throughout the network, and the formation of porous hydrogels.
32531008	3	47	theme	carboxymethyl	415:427	arg1	CMC					440:442	CMC	440:442	CMC	440:442	A thickening agent, carboxymethyl cellulose (CMC), is incorporated into a hydrogen bonding N-acryloyl-2-glycine (ACG) monomer and an initiator, and vortexing generates air bubbles in the viscous liquid.
32531008	3	47	theme	carboxymethyl	415:427	arg1	agent					408:412	A thickening agent	395:412	A thickening agent	395:412	A thickening agent, carboxymethyl cellulose (CMC), is incorporated into a hydrogen bonding N-acryloyl-2-glycine (ACG) monomer and an initiator, and vortexing generates air bubbles in the viscous liquid.
32531008	3	47	theme	carboxymethyl	415:427	arg1	cellulose					429:437	carboxymethyl cellulose	415:437	carboxymethyl cellulose (CMC)	415:443	A thickening agent, carboxymethyl cellulose (CMC), is incorporated into a hydrogen bonding N-acryloyl-2-glycine (ACG) monomer and an initiator, and vortexing generates air bubbles in the viscous liquid.
32531008	3	48	theme	thickening	397:406	arg1	agent					408:412	A thickening agent	395:412	A thickening agent	395:412	A thickening agent, carboxymethyl cellulose (CMC), is incorporated into a hydrogen bonding N-acryloyl-2-glycine (ACG) monomer and an initiator, and vortexing generates air bubbles in the viscous liquid.
32531008	3	48	theme	thickening	397:406	arg1	cellulose					429:437	carboxymethyl cellulose	415:437	carboxymethyl cellulose (CMC)	415:443	A thickening agent, carboxymethyl cellulose (CMC), is incorporated into a hydrogen bonding N-acryloyl-2-glycine (ACG) monomer and an initiator, and vortexing generates air bubbles in the viscous liquid.
32531008	8	49	from	emergency	1499:1507	arg1	applicable					1482:1491	applicable	1482:1491	applicable	1482:1491	This PACG sponge holds promising potential as a hemostatic agent applicable in an emergency.
32531008	6	50	dep	in	1046:1047	arg1	vitro					1049:1053	vitro	1049:1053	vitro	1049:1053	PACG/CMC sponges are shown to demonstrate a tunable liquid absorption ability, in vitro hemostatic ability, better hemocompatibility and cytocompatibility.
32531008	7	51	theme	high	1329:1332	arg1	ability					1351:1357	the high blood absorption ability	1325:1357	the high blood absorption ability	1325:1357	In a rat liver injury model and a femoral artery injury model, the PACG/CMC sponge can significantly reduce the bleeding time and blood loss compared with gauze and commercial gelatin sponge because of the high blood absorption ability and effective concentration of blood coagulation factors.
32531008	1	52	theme	hemostatic	92:101	arg1	sponge					103:108	a hemostatic sponge	90:108	a hemostatic sponge that can be used for treating both arterial hemorrhage and non-compressible bleeding	90:193	The development of a hemostatic sponge that can be used for treating both arterial hemorrhage and non-compressible bleeding remains a challenge.
32531008	0	53	theme	-based	35:40	arg1	sponge					42:47	A robust poly(N-acryloyl-2-glycine)-based sponge	0:47	A robust poly(N-acryloyl-2-glycine)-based sponge for rapid hemostasis	0:68	A robust poly(N-acryloyl-2-glycine)-based sponge for rapid hemostasis.
32531008	6	54	theme	absorption	1026:1035	arg1	ability					1037:1043	a tunable liquid absorption ability	1009:1043	a tunable liquid absorption ability	1009:1043	PACG/CMC sponges are shown to demonstrate a tunable liquid absorption ability, in vitro hemostatic ability, better hemocompatibility and cytocompatibility.
32531008	7	55	theme	absorption	1340:1349	arg1	ability					1351:1357	the high blood absorption ability	1325:1357	the high blood absorption ability	1325:1357	In a rat liver injury model and a femoral artery injury model, the PACG/CMC sponge can significantly reduce the bleeding time and blood loss compared with gauze and commercial gelatin sponge because of the high blood absorption ability and effective concentration of blood coagulation factors.
32531008	4	56	theme	hydrogels	774:782	arg1	aids					651:654	meanwhile aids	641:654	meanwhile aids	641:654	Heating initiates fast polymerization, and meanwhile aids in expanding of bubbles, which results in the fixation of bubbles throughout the network, and the formation of porous hydrogels.
32531008	4	56	theme	hydrogels	774:782	arg1	formation					754:762	the formation	750:762	the formation of porous hydrogels	750:782	Heating initiates fast polymerization, and meanwhile aids in expanding of bubbles, which results in the fixation of bubbles throughout the network, and the formation of porous hydrogels.
32531008	4	56	theme	hydrogels	774:782	arg1	polymerization					621:634	fast polymerization	616:634	fast polymerization	616:634	Heating initiates fast polymerization, and meanwhile aids in expanding of bubbles, which results in the fixation of bubbles throughout the network, and the formation of porous hydrogels.
32531008	7	57	theme	gelatin	1299:1305	arg1	sponge					1307:1312	commercial gelatin sponge	1288:1312	commercial gelatin sponge	1288:1312	In a rat liver injury model and a femoral artery injury model, the PACG/CMC sponge can significantly reduce the bleeding time and blood loss compared with gauze and commercial gelatin sponge because of the high blood absorption ability and effective concentration of blood coagulation factors.
32531008	1	58	theme	sponge	103:108	arg1	development					75:85	The development	71:85	The development of a hemostatic sponge that can be used for treating both arterial hemorrhage and non-compressible bleeding	71:193	The development of a hemostatic sponge that can be used for treating both arterial hemorrhage and non-compressible bleeding remains a challenge.
32531008	7	59	theme	factors	1408:1414	arg1	concentration					1373:1385	effective concentration	1363:1385	effective concentration of blood coagulation factors	1363:1414	In a rat liver injury model and a femoral artery injury model, the PACG/CMC sponge can significantly reduce the bleeding time and blood loss compared with gauze and commercial gelatin sponge because of the high blood absorption ability and effective concentration of blood coagulation factors.
32531008	7	59	theme	factors	1408:1414	arg1	ability					1351:1357	the high blood absorption ability	1325:1357	the high blood absorption ability	1325:1357	In a rat liver injury model and a femoral artery injury model, the PACG/CMC sponge can significantly reduce the bleeding time and blood loss compared with gauze and commercial gelatin sponge because of the high blood absorption ability and effective concentration of blood coagulation factors.
32531008	8	60	theme	promising	1440:1448	arg1	potential					1450:1458	promising potential	1440:1458	promising potential	1440:1458	This PACG sponge holds promising potential as a hemostatic agent applicable in an emergency.
32531008	8	60	theme	promising	1440:1448	arg1	agent					1476:1480	a hemostatic agent	1463:1480	a hemostatic agent applicable in an emergency	1463:1507	This PACG sponge holds promising potential as a hemostatic agent applicable in an emergency.
32531008	2	61	dep	strategy	349:356	arg1	in					324:325	in	324:325	in	324:325	In this work, we propose the fabrication of a robust hemostatic sponge by a hydrogen bond strengthening and in situ bubble expanding strategy in thermo-initiation polymerization.
32531008	7	62	theme	blood	1390:1394	arg1	factors					1408:1414	blood coagulation factors	1390:1414	blood coagulation factors	1390:1414	In a rat liver injury model and a femoral artery injury model, the PACG/CMC sponge can significantly reduce the bleeding time and blood loss compared with gauze and commercial gelatin sponge because of the high blood absorption ability and effective concentration of blood coagulation factors.
32531008	2	63	theme	hydrogen	292:299	arg1	bond					301:304	a hydrogen bond	290:304	a hydrogen bond strengthening and in situ bubble expanding strategy in thermo-initiation polymerization	290:392	In this work, we propose the fabrication of a robust hemostatic sponge by a hydrogen bond strengthening and in situ bubble expanding strategy in thermo-initiation polymerization.
32531008	5	64	theme	due	917:919	arg1	strengths					907:915	robust compressive strengths	888:915	robust compressive strengths due to the hydrogen bonding interactions of PACG	888:964	Further lyophilization of the foaming hydrogels leads to the final generation of PACG/CMC sponges with robust compressive strengths due to the hydrogen bonding interactions of PACG.
32531008	7	65	theme	effective	1363:1371	arg1	concentration					1373:1385	effective concentration	1363:1385	effective concentration of blood coagulation factors	1363:1414	In a rat liver injury model and a femoral artery injury model, the PACG/CMC sponge can significantly reduce the bleeding time and blood loss compared with gauze and commercial gelatin sponge because of the high blood absorption ability and effective concentration of blood coagulation factors.
32531008	5	66	theme	PACG	961:964	arg1	interactions					945:956	the hydrogen bonding interactions	924:956	the hydrogen bonding interactions of PACG	924:964	Further lyophilization of the foaming hydrogels leads to the final generation of PACG/CMC sponges with robust compressive strengths due to the hydrogen bonding interactions of PACG.
32531008	4	67	from	formation	754:762	arg1	expanding					659:667	expanding	659:667	expanding	659:667	Heating initiates fast polymerization, and meanwhile aids in expanding of bubbles, which results in the fixation of bubbles throughout the network, and the formation of porous hydrogels.
32531008	5	68	theme	robust	888:893	arg1	strengths					907:915	robust compressive strengths	888:915	robust compressive strengths due to the hydrogen bonding interactions of PACG	888:964	Further lyophilization of the foaming hydrogels leads to the final generation of PACG/CMC sponges with robust compressive strengths due to the hydrogen bonding interactions of PACG.
32531008	0	69	theme	rapid	53:57	arg1	hemostasis					59:68	rapid hemostasis	53:68	rapid hemostasis	53:68	A robust poly(N-acryloyl-2-glycine)-based sponge for rapid hemostasis.
32531008	5	70	theme	bonding	937:943	arg1	interactions					945:956	the hydrogen bonding interactions	924:956	the hydrogen bonding interactions of PACG	924:964	Further lyophilization of the foaming hydrogels leads to the final generation of PACG/CMC sponges with robust compressive strengths due to the hydrogen bonding interactions of PACG.
32531008	6	71	theme	liquid	1019:1024	arg1	ability					1037:1043	a tunable liquid absorption ability	1009:1043	a tunable liquid absorption ability	1009:1043	PACG/CMC sponges are shown to demonstrate a tunable liquid absorption ability, in vitro hemostatic ability, better hemocompatibility and cytocompatibility.
32531008	3	72	theme	N-acryloyl-2-glycine	486:505	arg1	monomer					513:519	a hydrogen bonding N-acryloyl-2-glycine (ACG) monomer	467:519	a hydrogen bonding N-acryloyl-2-glycine (ACG) monomer	467:519	A thickening agent, carboxymethyl cellulose (CMC), is incorporated into a hydrogen bonding N-acryloyl-2-glycine (ACG) monomer and an initiator, and vortexing generates air bubbles in the viscous liquid.
32531008	5	73	theme	hydrogels	823:831	arg1	lyophilization					793:806	Further lyophilization	785:806	Further lyophilization of the foaming hydrogels	785:831	Further lyophilization of the foaming hydrogels leads to the final generation of PACG/CMC sponges with robust compressive strengths due to the hydrogen bonding interactions of PACG.
32531008	7	74	theme	PACG/CMC	1190:1197	arg1	sponge					1199:1204	the PACG/CMC sponge	1186:1204	the PACG/CMC sponge	1186:1204	In a rat liver injury model and a femoral artery injury model, the PACG/CMC sponge can significantly reduce the bleeding time and blood loss compared with gauze and commercial gelatin sponge because of the high blood absorption ability and effective concentration of blood coagulation factors.
32531008	7	75	theme	injury	1138:1143	arg1	model					1145:1149	a rat liver injury model	1126:1149	a rat liver injury model	1126:1149	In a rat liver injury model and a femoral artery injury model, the PACG/CMC sponge can significantly reduce the bleeding time and blood loss compared with gauze and commercial gelatin sponge because of the high blood absorption ability and effective concentration of blood coagulation factors.
32531008	5	76	theme	final	846:850	arg1	generation					852:861	the final generation	842:861	the final generation of PACG/CMC sponges with robust compressive strengths due to the hydrogen bonding interactions of PACG	842:964	Further lyophilization of the foaming hydrogels leads to the final generation of PACG/CMC sponges with robust compressive strengths due to the hydrogen bonding interactions of PACG.
33504656	6	0	theme	polysaccharides	805:819	arg1	decrease					835:842	a smaller decrease	825:842	a smaller decrease of capsular polysaccharides	825:870	The kpsM mutant exhibits a significant reduction of released polysaccharides and a smaller decrease of capsular polysaccharides, but it accumulates more polyhydroxybutyrate (PHB) than the wild type.
33504656	6	0	theme	polysaccharides	805:819	arg1	reduction					783:791	a significant reduction	769:791	a significant reduction of released polysaccharides	769:819	The kpsM mutant exhibits a significant reduction of released polysaccharides and a smaller decrease of capsular polysaccharides, but it accumulates more polyhydroxybutyrate (PHB) than the wild type.
33504656	10	1	theme	biological	1987:1996	arg1	roles					1998:2002	different biological roles	1977:2002	different biological roles	1977:2002	The accumulation of PHB in cells of the mutant, without affecting significantly its fitness/growth rate, points to its possible use as a chassis for the production of compounds of interest.IMPORTANCE Most cyanobacteria produce extracellular polymeric substances (EPS) that fulfill different biological roles depending on the strain/environmental conditions.
33504656	12	2	theme	carbon	2466:2471	arg1	intracellularly					2473:2487	more carbon intracellularly	2461:2487	more carbon intracellularly	2461:2487	Here, we show that a Synechocystis kpsM (slr0977) mutant secretes less EPS than the wild type, accumulating more carbon intracellularly, as polyhydroxybutyrate.
33504656	15	3	theme	kpsM	2857:2860	arg1	disruption					2862:2871	kpsM disruption	2857:2871	kpsM disruption	2857:2871	Altogether, this work provides a comprehensive overview of the impact of kpsM disruption on Synechocystis physiology, highlighting the importance of EPS as a carbon sink and showing how cells adapt when their secretion is impaired, and the redirection of the carbon fluxes.
33504656	15	4	theme	impact	2847:2852	arg1	overview					2831:2838	a comprehensive overview	2815:2838	a comprehensive overview of the impact of kpsM disruption on Synechocystis physiology, highlighting the importance of EPS as a carbon sink and showing how cells adapt when their secretion is impaired,	2815:3014	Altogether, this work provides a comprehensive overview of the impact of kpsM disruption on Synechocystis physiology, highlighting the importance of EPS as a carbon sink and showing how cells adapt when their secretion is impaired, and the redirection of the carbon fluxes.
33504656	15	4	theme	impact	2847:2852	arg1	redirection					3024:3034	the redirection	3020:3034	the redirection of the carbon fluxes	3020:3055	Altogether, this work provides a comprehensive overview of the impact of kpsM disruption on Synechocystis physiology, highlighting the importance of EPS as a carbon sink and showing how cells adapt when their secretion is impaired, and the redirection of the carbon fluxes.
33504656	7	5	theme	clumping	1005:1012	arg1	phenotype					1014:1022	a light/cell density-dependent clumping phenotype	974:1022	a light/cell density-dependent clumping phenotype	974:1022	In addition, this strain shows a light/cell density-dependent clumping phenotype and exhibits an altered protein secretion capacity.
33504656	13	6	theme	modified	2628:2635	arg1	glycosylation					2637:2649	modified glycosylation	2628:2649	modified glycosylation of PilA	2628:2657	Further characterization showed a light/cell density-dependent clumping phenotype, altered protein secretion, and modified glycosylation of PilA.
33504656	6	7	theme	significant	771:781	arg1	reduction					783:791	a significant reduction	769:791	a significant reduction of released polysaccharides	769:819	The kpsM mutant exhibits a significant reduction of released polysaccharides and a smaller decrease of capsular polysaccharides, but it accumulates more polyhydroxybutyrate (PHB) than the wild type.
33504656	9	8	theme	impaired	1547:1554	arg1	secretion					1560:1568	impaired EPS secretion	1547:1568	impaired EPS secretion	1547:1568	Proteomic and transcriptomic analyses revealed significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism, in response to the inactivation of slr0977 Overall, this work shows for the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments, highlighting the importance of EPS as a major carbon sink in cyanobacteria.
33504656	7	9	theme	light/cell	976:985	arg1	phenotype					1014:1022	a light/cell density-dependent clumping phenotype	974:1022	a light/cell density-dependent clumping phenotype	974:1022	In addition, this strain shows a light/cell density-dependent clumping phenotype and exhibits an altered protein secretion capacity.
33504656	11	10	theme	EPS	2089:2091	arg1	pathways					2110:2117	the cyanobacterial EPS synthesis/export pathways	2070:2117	the cyanobacterial EPS synthesis/export pathways	2070:2117	The interest in the cyanobacterial EPS synthesis/export pathways has been increasing, not only to optimize EPS production but also to efficiently redirect carbon flux toward the production of other compounds, allowing the implementation of industrial systems based on cyanobacterial cell factories.
33504656	9	11	theme	energy	1336:1341	arg1	production					1343:1352	energy production	1336:1352	energy production	1336:1352	Proteomic and transcriptomic analyses revealed significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism, in response to the inactivation of slr0977 Overall, this work shows for the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments, highlighting the importance of EPS as a major carbon sink in cyanobacteria.
33504656	15	12	theme	comprehensive	2817:2829	arg1	overview					2831:2838	a comprehensive overview	2815:2838	a comprehensive overview of the impact of kpsM disruption on Synechocystis physiology, highlighting the importance of EPS as a carbon sink and showing how cells adapt when their secretion is impaired,	2815:3014	Altogether, this work provides a comprehensive overview of the impact of kpsM disruption on Synechocystis physiology, highlighting the importance of EPS as a carbon sink and showing how cells adapt when their secretion is impaired, and the redirection of the carbon fluxes.
33504656	13	13	theme	protein	2605:2611	arg1	secretion					2613:2621	altered protein secretion	2597:2621	altered protein secretion	2597:2621	Further characterization showed a light/cell density-dependent clumping phenotype, altered protein secretion, and modified glycosylation of PilA.
33504656	8	14	theme	important	1098:1106	arg1	component					1119:1127	the most important structural component	1089:1127	the most important structural component of pili	1089:1135	Furthermore, the most important structural component of pili, the protein PilA, was found to have a modified glycosylation pattern in the mutant compared to the wild type.
33504656	8	14	theme	important	1098:1106	arg1	PilA					1150:1153	the protein PilA	1138:1153	the protein PilA	1138:1153	Furthermore, the most important structural component of pili, the protein PilA, was found to have a modified glycosylation pattern in the mutant compared to the wild type.
33504656	15	15	theme	carbon	3043:3048	arg1	fluxes					3050:3055	the carbon fluxes	3039:3055	the carbon fluxes	3039:3055	Altogether, this work provides a comprehensive overview of the impact of kpsM disruption on Synechocystis physiology, highlighting the importance of EPS as a carbon sink and showing how cells adapt when their secretion is impaired, and the redirection of the carbon fluxes.
33504656	9	16	theme	transcriptomic	1578:1591	arg1	adjustments					1607:1617	transcriptomic and proteomic adjustments	1578:1617	transcriptomic and proteomic adjustments	1578:1617	Proteomic and transcriptomic analyses revealed significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism, in response to the inactivation of slr0977 Overall, this work shows for the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments, highlighting the importance of EPS as a major carbon sink in cyanobacteria.
33504656	14	17	theme	carbon	2765:2770	arg1	metabolism					2772:2781	carbon metabolism	2765:2781	carbon metabolism	2765:2781	The proteome and transcriptome of the mutant revealed significant changes, namely, in photosynthesis and carbon metabolism.
33504656	12	18	theme	Synechocystis	2374:2386	arg1	mutant					2403:2408	a Synechocystis kpsM (slr0977) mutant	2372:2408	a Synechocystis kpsM (slr0977) mutant	2372:2408	Here, we show that a Synechocystis kpsM (slr0977) mutant secretes less EPS than the wild type, accumulating more carbon intracellularly, as polyhydroxybutyrate.
33504656	6	19	theme	capsular	847:854	arg1	polysaccharides					856:870	capsular polysaccharides	847:870	capsular polysaccharides	847:870	The kpsM mutant exhibits a significant reduction of released polysaccharides and a smaller decrease of capsular polysaccharides, but it accumulates more polyhydroxybutyrate (PHB) than the wild type.
33504656	9	20	theme	proteomic	1597:1605	arg1	adjustments					1607:1617	transcriptomic and proteomic adjustments	1578:1617	transcriptomic and proteomic adjustments	1578:1617	Proteomic and transcriptomic analyses revealed significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism, in response to the inactivation of slr0977 Overall, this work shows for the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments, highlighting the importance of EPS as a major carbon sink in cyanobacteria.
33504656	11	21	theme	compounds	2252:2260	arg1	production					2232:2241	the production	2228:2241	the production of other compounds	2228:2260	The interest in the cyanobacterial EPS synthesis/export pathways has been increasing, not only to optimize EPS production but also to efficiently redirect carbon flux toward the production of other compounds, allowing the implementation of industrial systems based on cyanobacterial cell factories.
33504656	11	22	theme	carbon	2209:2214	arg1	flux					2216:2219	carbon flux	2209:2219	carbon flux	2209:2219	The interest in the cyanobacterial EPS synthesis/export pathways has been increasing, not only to optimize EPS production but also to efficiently redirect carbon flux toward the production of other compounds, allowing the implementation of industrial systems based on cyanobacterial cell factories.
33504656	4	23	theme	EPS-related	572:582	arg1	KpsM					593:596	KpsM	593:596	KpsM (Slr0977)	593:606	Here, we show that the absence of a putative EPS-related protein, KpsM (Slr0977), has a pleiotropic effect on Synechocystis sp.
33504656	4	23	theme	EPS-related	572:582	arg1	protein					584:590	a putative EPS-related protein	561:590	a putative EPS-related protein	561:590	Here, we show that the absence of a putative EPS-related protein, KpsM (Slr0977), has a pleiotropic effect on Synechocystis sp.
33504656	10	24	theme	compounds	1863:1871	arg1	production					1849:1858	the production	1845:1858	the production of compounds of interest.IMPORTANCE Most cyanobacteria	1845:1913	The accumulation of PHB in cells of the mutant, without affecting significantly its fitness/growth rate, points to its possible use as a chassis for the production of compounds of interest.IMPORTANCE Most cyanobacteria produce extracellular polymeric substances (EPS) that fulfill different biological roles depending on the strain/environmental conditions.
33504656	0	25	theme	Carbon	104:109	arg1	Fluxes					111:116	Carbon Fluxes	104:116	Carbon Fluxes in Synechocystis sp	104:136	Absence of KpsM (Slr0977) Impairs the Secretion of Extracellular Polymeric Substances (EPS) and Impacts Carbon Fluxes in Synechocystis sp.
33504656	13	26	theme	Further	2514:2520	arg1	characterization					2522:2537	Further characterization	2514:2537	Further characterization	2514:2537	Further characterization showed a light/cell density-dependent clumping phenotype, altered protein secretion, and modified glycosylation of PilA.
33504656	5	27	theme	fluxes	736:741	arg1	export					711:716	the export	707:716	the export of EPS and carbon fluxes	707:741	strain PCC 6803 physiology, with a strong impact on the export of EPS and carbon fluxes.
33504656	8	28	theme	pili	1132:1135	arg1	component					1119:1127	the most important structural component	1089:1127	the most important structural component of pili	1089:1135	Furthermore, the most important structural component of pili, the protein PilA, was found to have a modified glycosylation pattern in the mutant compared to the wild type.
33504656	8	28	theme	pili	1132:1135	arg1	PilA					1150:1153	the protein PilA	1138:1153	the protein PilA	1138:1153	Furthermore, the most important structural component of pili, the protein PilA, was found to have a modified glycosylation pattern in the mutant compared to the wild type.
33504656	4	29	theme	pleiotropic	615:625	arg1	effect					627:632	a pleiotropic effect	613:632	a pleiotropic effect on Synechocystis sp	613:652	Here, we show that the absence of a putative EPS-related protein, KpsM (Slr0977), has a pleiotropic effect on Synechocystis sp.
33504656	8	30	contain	have	1169:1172	arg1	component					1119:1127	the most important structural component	1089:1127	the most important structural component of pili	1089:1135	Furthermore, the most important structural component of pili, the protein PilA, was found to have a modified glycosylation pattern in the mutant compared to the wild type.
33504656	8	30	contain	have	1169:1172	arg1	PilA					1150:1153	the protein PilA	1138:1153	the protein PilA	1138:1153	Furthermore, the most important structural component of pili, the protein PilA, was found to have a modified glycosylation pattern in the mutant compared to the wild type.
33504656	8	30	contain	have	1169:1172	arg2	pattern					1199:1205	a modified glycosylation pattern	1174:1205	a modified glycosylation pattern	1174:1205	Furthermore, the most important structural component of pili, the protein PilA, was found to have a modified glycosylation pattern in the mutant compared to the wild type.
33504656	8	31	theme	glycosylation	1185:1197	arg1	pattern					1199:1205	a modified glycosylation pattern	1174:1205	a modified glycosylation pattern	1174:1205	Furthermore, the most important structural component of pili, the protein PilA, was found to have a modified glycosylation pattern in the mutant compared to the wild type.
33504656	10	32	theme	cyanobacteria	1901:1913	arg1	compounds					1863:1871	compounds	1863:1871	compounds of interest.IMPORTANCE Most cyanobacteria	1863:1913	The accumulation of PHB in cells of the mutant, without affecting significantly its fitness/growth rate, points to its possible use as a chassis for the production of compounds of interest.IMPORTANCE Most cyanobacteria produce extracellular polymeric substances (EPS) that fulfill different biological roles depending on the strain/environmental conditions.
33504656	9	33	theme	phosphorylation	1404:1418	arg1	mechanisms					1322:1331	the mechanisms	1318:1331	the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism	1318:1441	Proteomic and transcriptomic analyses revealed significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism, in response to the inactivation of slr0977 Overall, this work shows for the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments, highlighting the importance of EPS as a major carbon sink in cyanobacteria.
33504656	5	34	with	PCC	662:664	arg1	impact					697:702	a strong impact	688:702	a strong impact on the export of EPS and carbon fluxes	688:741	strain PCC 6803 physiology, with a strong impact on the export of EPS and carbon fluxes.
33504656	10	35	theme	interest.IMPORTANCE	1876:1894	arg1	cyanobacteria					1901:1913	interest.IMPORTANCE Most cyanobacteria	1876:1913	interest.IMPORTANCE Most cyanobacteria	1876:1913	The accumulation of PHB in cells of the mutant, without affecting significantly its fitness/growth rate, points to its possible use as a chassis for the production of compounds of interest.IMPORTANCE Most cyanobacteria produce extracellular polymeric substances (EPS) that fulfill different biological roles depending on the strain/environmental conditions.
33504656	0	36	theme	Extracellular	51:63	arg1	EPS					87:89	EPS	87:89	EPS	87:89	Absence of KpsM (Slr0977) Impairs the Secretion of Extracellular Polymeric Substances (EPS) and Impacts Carbon Fluxes in Synechocystis sp.
33504656	0	36	theme	Extracellular	51:63	arg1	Substances					75:84	Extracellular Polymeric Substances	51:84	Extracellular Polymeric Substances (EPS)	51:90	Absence of KpsM (Slr0977) Impairs the Secretion of Extracellular Polymeric Substances (EPS) and Impacts Carbon Fluxes in Synechocystis sp.
33504656	9	37	theme	metabolism	1432:1441	arg1	mechanisms					1322:1331	the mechanisms	1318:1331	the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism	1318:1441	Proteomic and transcriptomic analyses revealed significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism, in response to the inactivation of slr0977 Overall, this work shows for the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments, highlighting the importance of EPS as a major carbon sink in cyanobacteria.
33504656	15	38	from	impact	2847:2852	arg1	physiology					2890:2899	Synechocystis physiology	2876:2899	Synechocystis physiology	2876:2899	Altogether, this work provides a comprehensive overview of the impact of kpsM disruption on Synechocystis physiology, highlighting the importance of EPS as a carbon sink and showing how cells adapt when their secretion is impaired, and the redirection of the carbon fluxes.
33504656	6	39	theme	more	892:895	arg1	PHB					918:920	PHB	918:920	PHB	918:920	The kpsM mutant exhibits a significant reduction of released polysaccharides and a smaller decrease of capsular polysaccharides, but it accumulates more polyhydroxybutyrate (PHB) than the wild type.
33504656	6	39	theme	more	892:895	arg1	polyhydroxybutyrate					897:915	more polyhydroxybutyrate	892:915	more polyhydroxybutyrate (PHB) than the wild type	892:940	The kpsM mutant exhibits a significant reduction of released polysaccharides and a smaller decrease of capsular polysaccharides, but it accumulates more polyhydroxybutyrate (PHB) than the wild type.
33504656	9	40	theme	major	1660:1664	arg1	sink					1673:1676	a major carbon sink	1658:1676	a major carbon sink in cyanobacteria	1658:1693	Proteomic and transcriptomic analyses revealed significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism, in response to the inactivation of slr0977 Overall, this work shows for the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments, highlighting the importance of EPS as a major carbon sink in cyanobacteria.
33504656	9	40	theme	major	1660:1664	arg1	importance					1637:1646	the importance	1633:1646	the importance of EPS	1633:1653	Proteomic and transcriptomic analyses revealed significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism, in response to the inactivation of slr0977 Overall, this work shows for the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments, highlighting the importance of EPS as a major carbon sink in cyanobacteria.
33504656	11	41	theme	systems	2305:2311	arg1	implementation					2276:2289	the implementation	2272:2289	the implementation of industrial systems based on cyanobacterial cell factories	2272:2350	The interest in the cyanobacterial EPS synthesis/export pathways has been increasing, not only to optimize EPS production but also to efficiently redirect carbon flux toward the production of other compounds, allowing the implementation of industrial systems based on cyanobacterial cell factories.
33504656	10	42	theme	extracellular	1923:1935	arg1	EPS					1959:1961	EPS	1959:1961	EPS	1959:1961	The accumulation of PHB in cells of the mutant, without affecting significantly its fitness/growth rate, points to its possible use as a chassis for the production of compounds of interest.IMPORTANCE Most cyanobacteria produce extracellular polymeric substances (EPS) that fulfill different biological roles depending on the strain/environmental conditions.
33504656	10	42	theme	extracellular	1923:1935	arg1	substances					1947:1956	extracellular polymeric substances	1923:1956	extracellular polymeric substances (EPS) that fulfill different biological roles depending on the strain/environmental conditions	1923:2051	The accumulation of PHB in cells of the mutant, without affecting significantly its fitness/growth rate, points to its possible use as a chassis for the production of compounds of interest.IMPORTANCE Most cyanobacteria produce extracellular polymeric substances (EPS) that fulfill different biological roles depending on the strain/environmental conditions.
33504656	0	43	theme	Substances	75:84	arg1	Secretion					38:46	the Secretion	34:46	the Secretion of Extracellular Polymeric Substances (EPS)	34:90	Absence of KpsM (Slr0977) Impairs the Secretion of Extracellular Polymeric Substances (EPS) and Impacts Carbon Fluxes in Synechocystis sp.
33504656	8	44	theme	wild	1237:1240	arg1	type					1242:1245	the wild type	1233:1245	the wild type	1233:1245	Furthermore, the most important structural component of pili, the protein PilA, was found to have a modified glycosylation pattern in the mutant compared to the wild type.
33504656	2	45	theme	biofilm	353:359	arg1	formation					361:369	biofilm formation	353:369	biofilm formation	353:369	Many cyanobacteria produce extracellular polymeric substances (EPS), composed mainly of heteropolysaccharides, that play a variety of physiological roles, being crucial for cell protection, motility, and biofilm formation.
33504656	5	46	theme	strain	655:660	arg1	PCC					662:664	strain PCC 6803	655:669	strain PCC 6803	655:669	strain PCC 6803 physiology, with a strong impact on the export of EPS and carbon fluxes.
33504656	10	47	from	accumulation	1700:1711	arg1	cells					1723:1727	cells	1723:1727	cells of the mutant	1723:1741	The accumulation of PHB in cells of the mutant, without affecting significantly its fitness/growth rate, points to its possible use as a chassis for the production of compounds of interest.IMPORTANCE Most cyanobacteria produce extracellular polymeric substances (EPS) that fulfill different biological roles depending on the strain/environmental conditions.
33504656	11	48	theme	cyanobacterial	2074:2087	arg1	pathways					2110:2117	the cyanobacterial EPS synthesis/export pathways	2070:2117	the cyanobacterial EPS synthesis/export pathways	2070:2117	The interest in the cyanobacterial EPS synthesis/export pathways has been increasing, not only to optimize EPS production but also to efficiently redirect carbon flux toward the production of other compounds, allowing the implementation of industrial systems based on cyanobacterial cell factories.
33504656	11	49	theme	cell	2337:2340	arg1	factories					2342:2350	cyanobacterial cell factories	2322:2350	cyanobacterial cell factories	2322:2350	The interest in the cyanobacterial EPS synthesis/export pathways has been increasing, not only to optimize EPS production but also to efficiently redirect carbon flux toward the production of other compounds, allowing the implementation of industrial systems based on cyanobacterial cell factories.
33504656	15	50	from	physiology	2890:2899	arg1	overview					2831:2838	a comprehensive overview	2815:2838	a comprehensive overview of the impact of kpsM disruption on Synechocystis physiology, highlighting the importance of EPS as a carbon sink and showing how cells adapt when their secretion is impaired,	2815:3014	Altogether, this work provides a comprehensive overview of the impact of kpsM disruption on Synechocystis physiology, highlighting the importance of EPS as a carbon sink and showing how cells adapt when their secretion is impaired, and the redirection of the carbon fluxes.
33504656	15	50	from	physiology	2890:2899	arg1	redirection					3024:3034	the redirection	3020:3034	the redirection of the carbon fluxes	3020:3055	Altogether, this work provides a comprehensive overview of the impact of kpsM disruption on Synechocystis physiology, highlighting the importance of EPS as a carbon sink and showing how cells adapt when their secretion is impaired, and the redirection of the carbon fluxes.
33504656	15	51	theme	carbon	2942:2947	arg1	importance					2919:2928	the importance	2915:2928	the importance of EPS	2915:2935	Altogether, this work provides a comprehensive overview of the impact of kpsM disruption on Synechocystis physiology, highlighting the importance of EPS as a carbon sink and showing how cells adapt when their secretion is impaired, and the redirection of the carbon fluxes.
33504656	15	51	theme	carbon	2942:2947	arg1	sink					2949:2952	a carbon sink	2940:2952	a carbon sink	2940:2952	Altogether, this work provides a comprehensive overview of the impact of kpsM disruption on Synechocystis physiology, highlighting the importance of EPS as a carbon sink and showing how cells adapt when their secretion is impaired, and the redirection of the carbon fluxes.
33504656	14	52	theme	significant	2714:2724	arg1	changes					2726:2732	significant changes	2714:2732	significant changes	2714:2732	The proteome and transcriptome of the mutant revealed significant changes, namely, in photosynthesis and carbon metabolism.
33504656	3	53	theme	EPS	410:412	arg1	pathways					427:434	the EPS biosynthetic pathways	406:434	the EPS biosynthetic pathways as well as their assembly and export mechanisms	406:482	However, due to their complexity, the EPS biosynthetic pathways as well as their assembly and export mechanisms are still far from being fully understood.
33504656	0	54	theme	KpsM	11:14	arg1	Absence					0:6	Absence	0:6	Absence of KpsM (Slr0977)	0:24	Absence of KpsM (Slr0977) Impairs the Secretion of Extracellular Polymeric Substances (EPS) and Impacts Carbon Fluxes in Synechocystis sp.
33504656	9	55	from	sink	1673:1676	arg1	cyanobacteria					1681:1693	cyanobacteria	1681:1693	cyanobacteria	1681:1693	Proteomic and transcriptomic analyses revealed significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism, in response to the inactivation of slr0977 Overall, this work shows for the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments, highlighting the importance of EPS as a major carbon sink in cyanobacteria.
33504656	9	56	theme	slr0977	1479:1485	arg1	inactivation					1463:1474	the inactivation	1459:1474	the inactivation of slr0977 Overall	1459:1493	Proteomic and transcriptomic analyses revealed significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism, in response to the inactivation of slr0977 Overall, this work shows for the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments, highlighting the importance of EPS as a major carbon sink in cyanobacteria.
33504656	10	57	theme	mutant	1736:1741	arg1	cells					1723:1727	cells	1723:1727	cells of the mutant	1723:1741	The accumulation of PHB in cells of the mutant, without affecting significantly its fitness/growth rate, points to its possible use as a chassis for the production of compounds of interest.IMPORTANCE Most cyanobacteria produce extracellular polymeric substances (EPS) that fulfill different biological roles depending on the strain/environmental conditions.
33504656	7	58	theme	protein	1048:1054	arg1	capacity					1066:1073	an altered protein secretion capacity	1037:1073	an altered protein secretion capacity	1037:1073	In addition, this strain shows a light/cell density-dependent clumping phenotype and exhibits an altered protein secretion capacity.
33504656	11	59	theme	synthesis/export	2093:2108	arg1	pathways					2110:2117	the cyanobacterial EPS synthesis/export pathways	2070:2117	the cyanobacterial EPS synthesis/export pathways	2070:2117	The interest in the cyanobacterial EPS synthesis/export pathways has been increasing, not only to optimize EPS production but also to efficiently redirect carbon flux toward the production of other compounds, allowing the implementation of industrial systems based on cyanobacterial cell factories.
33504656	9	60	theme	Proteomic	1248:1256	arg1	analyses					1277:1284	Proteomic and transcriptomic analyses	1248:1284	Proteomic and transcriptomic analyses	1248:1284	Proteomic and transcriptomic analyses revealed significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism, in response to the inactivation of slr0977 Overall, this work shows for the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments, highlighting the importance of EPS as a major carbon sink in cyanobacteria.
33504656	2	61	theme	extracellular	176:188	arg1	EPS					212:214	EPS	212:214	EPS	212:214	Many cyanobacteria produce extracellular polymeric substances (EPS), composed mainly of heteropolysaccharides, that play a variety of physiological roles, being crucial for cell protection, motility, and biofilm formation.
33504656	2	61	theme	extracellular	176:188	arg1	substances					200:209	extracellular polymeric substances	176:209	extracellular polymeric substances (EPS)	176:215	Many cyanobacteria produce extracellular polymeric substances (EPS), composed mainly of heteropolysaccharides, that play a variety of physiological roles, being crucial for cell protection, motility, and biofilm formation.
33504656	4	62	from	effect	627:632	arg1	sp					651:652	Synechocystis sp	637:652	Synechocystis sp	637:652	Here, we show that the absence of a putative EPS-related protein, KpsM (Slr0977), has a pleiotropic effect on Synechocystis sp.
33504656	9	63	theme	transcriptomic	1262:1275	arg1	analyses					1277:1284	Proteomic and transcriptomic analyses	1248:1284	Proteomic and transcriptomic analyses	1248:1284	Proteomic and transcriptomic analyses revealed significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism, in response to the inactivation of slr0977 Overall, this work shows for the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments, highlighting the importance of EPS as a major carbon sink in cyanobacteria.
33504656	9	64	with	cells	1536:1540	arg1	secretion					1560:1568	impaired EPS secretion	1547:1568	impaired EPS secretion	1547:1568	Proteomic and transcriptomic analyses revealed significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism, in response to the inactivation of slr0977 Overall, this work shows for the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments, highlighting the importance of EPS as a major carbon sink in cyanobacteria.
33504656	9	65	theme	first	1520:1524	arg1	time					1526:1529	the first time	1516:1529	the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments	1516:1617	Proteomic and transcriptomic analyses revealed significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism, in response to the inactivation of slr0977 Overall, this work shows for the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments, highlighting the importance of EPS as a major carbon sink in cyanobacteria.
33504656	4	66	contain	has	609:611	arg2	effect					627:632	a pleiotropic effect	613:632	a pleiotropic effect on Synechocystis sp	613:652	Here, we show that the absence of a putative EPS-related protein, KpsM (Slr0977), has a pleiotropic effect on Synechocystis sp.
33504656	4	66	contain	has	609:611	arg1	absence					550:556	the absence	546:556	the absence of a putative EPS-related protein, KpsM (Slr0977),	546:607	Here, we show that the absence of a putative EPS-related protein, KpsM (Slr0977), has a pleiotropic effect on Synechocystis sp.
33504656	14	67	from	changes	2726:2732	arg1	metabolism					2772:2781	carbon metabolism	2765:2781	carbon metabolism	2765:2781	The proteome and transcriptome of the mutant revealed significant changes, namely, in photosynthesis and carbon metabolism.
33504656	14	67	from	changes	2726:2732	arg1	photosynthesis					2746:2759	photosynthesis	2746:2759	photosynthesis	2746:2759	The proteome and transcriptome of the mutant revealed significant changes, namely, in photosynthesis and carbon metabolism.
33504656	2	68	theme	cell	322:325	arg1	protection					327:336	cell protection	322:336	cell protection	322:336	Many cyanobacteria produce extracellular polymeric substances (EPS), composed mainly of heteropolysaccharides, that play a variety of physiological roles, being crucial for cell protection, motility, and biofilm formation.
33504656	6	69	theme	released	796:803	arg1	polysaccharides					805:819	released polysaccharides	796:819	released polysaccharides	796:819	The kpsM mutant exhibits a significant reduction of released polysaccharides and a smaller decrease of capsular polysaccharides, but it accumulates more polyhydroxybutyrate (PHB) than the wild type.
33504656	10	70	theme	different	1977:1985	arg1	roles					1998:2002	different biological roles	1977:2002	different biological roles	1977:2002	The accumulation of PHB in cells of the mutant, without affecting significantly its fitness/growth rate, points to its possible use as a chassis for the production of compounds of interest.IMPORTANCE Most cyanobacteria produce extracellular polymeric substances (EPS) that fulfill different biological roles depending on the strain/environmental conditions.
33504656	10	71	theme	fitness/growth	1780:1793	arg1	rate					1795:1798	its fitness/growth rate	1776:1798	its fitness/growth rate	1776:1798	The accumulation of PHB in cells of the mutant, without affecting significantly its fitness/growth rate, points to its possible use as a chassis for the production of compounds of interest.IMPORTANCE Most cyanobacteria produce extracellular polymeric substances (EPS) that fulfill different biological roles depending on the strain/environmental conditions.
33504656	7	72	theme	density-dependent	987:1003	arg1	phenotype					1014:1022	a light/cell density-dependent clumping phenotype	974:1022	a light/cell density-dependent clumping phenotype	974:1022	In addition, this strain shows a light/cell density-dependent clumping phenotype and exhibits an altered protein secretion capacity.
33504656	10	73	theme	strain/environmental	2021:2040	arg1	conditions					2042:2051	the strain/environmental conditions	2017:2051	the strain/environmental conditions	2017:2051	The accumulation of PHB in cells of the mutant, without affecting significantly its fitness/growth rate, points to its possible use as a chassis for the production of compounds of interest.IMPORTANCE Most cyanobacteria produce extracellular polymeric substances (EPS) that fulfill different biological roles depending on the strain/environmental conditions.
33504656	10	74	theme	possible	1815:1822	arg1	use					1824:1826	its possible use	1811:1826	its possible use as a chassis for the production of compounds of interest.IMPORTANCE Most cyanobacteria	1811:1913	The accumulation of PHB in cells of the mutant, without affecting significantly its fitness/growth rate, points to its possible use as a chassis for the production of compounds of interest.IMPORTANCE Most cyanobacteria produce extracellular polymeric substances (EPS) that fulfill different biological roles depending on the strain/environmental conditions.
33504656	15	75	dep	physiology	2890:2899	arg1	showing					2958:2964	showing	2958:2964	showing how cells adapt when their secretion is impaired	2958:3013	Altogether, this work provides a comprehensive overview of the impact of kpsM disruption on Synechocystis physiology, highlighting the importance of EPS as a carbon sink and showing how cells adapt when their secretion is impaired, and the redirection of the carbon fluxes.
33504656	15	75	dep	physiology	2890:2899	arg1	highlighting					2902:2913	highlighting	2902:2913	highlighting the importance of EPS as a carbon sink	2902:2952	Altogether, this work provides a comprehensive overview of the impact of kpsM disruption on Synechocystis physiology, highlighting the importance of EPS as a carbon sink and showing how cells adapt when their secretion is impaired, and the redirection of the carbon fluxes.
33504656	9	76	theme	EPS	1556:1558	arg1	secretion					1560:1568	impaired EPS secretion	1547:1568	impaired EPS secretion	1547:1568	Proteomic and transcriptomic analyses revealed significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism, in response to the inactivation of slr0977 Overall, this work shows for the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments, highlighting the importance of EPS as a major carbon sink in cyanobacteria.
33504656	4	77	theme	protein	584:590	arg1	absence					550:556	the absence	546:556	the absence of a putative EPS-related protein, KpsM (Slr0977),	546:607	Here, we show that the absence of a putative EPS-related protein, KpsM (Slr0977), has a pleiotropic effect on Synechocystis sp.
33504656	13	78	theme	altered	2597:2603	arg1	secretion					2613:2621	altered protein secretion	2597:2621	altered protein secretion	2597:2621	Further characterization showed a light/cell density-dependent clumping phenotype, altered protein secretion, and modified glycosylation of PilA.
33504656	2	79	theme	physiological	283:295	arg1	roles					297:301	physiological roles	283:301	physiological roles	283:301	Many cyanobacteria produce extracellular polymeric substances (EPS), composed mainly of heteropolysaccharides, that play a variety of physiological roles, being crucial for cell protection, motility, and biofilm formation.
33504656	13	80	theme	PilA	2654:2657	arg1	phenotype					2586:2594	a light/cell density-dependent clumping phenotype	2546:2594	a light/cell density-dependent clumping phenotype	2546:2594	Further characterization showed a light/cell density-dependent clumping phenotype, altered protein secretion, and modified glycosylation of PilA.
33504656	13	80	theme	PilA	2654:2657	arg1	secretion					2613:2621	altered protein secretion	2597:2621	altered protein secretion	2597:2621	Further characterization showed a light/cell density-dependent clumping phenotype, altered protein secretion, and modified glycosylation of PilA.
33504656	13	80	theme	PilA	2654:2657	arg1	glycosylation					2637:2649	modified glycosylation	2628:2649	modified glycosylation of PilA	2628:2657	Further characterization showed a light/cell density-dependent clumping phenotype, altered protein secretion, and modified glycosylation of PilA.
33504656	9	81	theme	production	1343:1352	arg1	mechanisms					1322:1331	the mechanisms	1318:1331	the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism	1318:1441	Proteomic and transcriptomic analyses revealed significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism, in response to the inactivation of slr0977 Overall, this work shows for the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments, highlighting the importance of EPS as a major carbon sink in cyanobacteria.
33504656	12	82	theme	kpsM	2388:2391	arg1	mutant					2403:2408	a Synechocystis kpsM (slr0977) mutant	2372:2408	a Synechocystis kpsM (slr0977) mutant	2372:2408	Here, we show that a Synechocystis kpsM (slr0977) mutant secretes less EPS than the wild type, accumulating more carbon intracellularly, as polyhydroxybutyrate.
33504656	8	83	theme	protein	1142:1148	arg1	component					1119:1127	the most important structural component	1089:1127	the most important structural component of pili	1089:1135	Furthermore, the most important structural component of pili, the protein PilA, was found to have a modified glycosylation pattern in the mutant compared to the wild type.
33504656	8	83	theme	protein	1142:1148	arg1	PilA					1150:1153	the protein PilA	1138:1153	the protein PilA	1138:1153	Furthermore, the most important structural component of pili, the protein PilA, was found to have a modified glycosylation pattern in the mutant compared to the wild type.
33504656	9	84	theme	conversion	1358:1367	arg1	mechanisms					1322:1331	the mechanisms	1318:1331	the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism	1318:1441	Proteomic and transcriptomic analyses revealed significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism, in response to the inactivation of slr0977 Overall, this work shows for the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments, highlighting the importance of EPS as a major carbon sink in cyanobacteria.
33504656	6	85	theme	polysaccharides	856:870	arg1	decrease					835:842	a smaller decrease	825:842	a smaller decrease of capsular polysaccharides	825:870	The kpsM mutant exhibits a significant reduction of released polysaccharides and a smaller decrease of capsular polysaccharides, but it accumulates more polyhydroxybutyrate (PHB) than the wild type.
33504656	6	85	theme	polysaccharides	856:870	arg1	reduction					783:791	a significant reduction	769:791	a significant reduction of released polysaccharides	769:819	The kpsM mutant exhibits a significant reduction of released polysaccharides and a smaller decrease of capsular polysaccharides, but it accumulates more polyhydroxybutyrate (PHB) than the wild type.
33504656	9	86	dep	revealed	1286:1293	arg1	shows					1506:1510	shows	1506:1510	shows	1506:1510	Proteomic and transcriptomic analyses revealed significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism, in response to the inactivation of slr0977 Overall, this work shows for the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments, highlighting the importance of EPS as a major carbon sink in cyanobacteria.
33504656	11	87	from	interest	2058:2065	arg1	pathways					2110:2117	the cyanobacterial EPS synthesis/export pathways	2070:2117	the cyanobacterial EPS synthesis/export pathways	2070:2117	The interest in the cyanobacterial EPS synthesis/export pathways has been increasing, not only to optimize EPS production but also to efficiently redirect carbon flux toward the production of other compounds, allowing the implementation of industrial systems based on cyanobacterial cell factories.
33504656	14	88	dep	proteome	2664:2671	arg1	The					2660:2662	The	2660:2662	The	2660:2662	The proteome and transcriptome of the mutant revealed significant changes, namely, in photosynthesis and carbon metabolism.
33504656	5	89	theme	carbon	729:734	arg1	fluxes					736:741	carbon fluxes	729:741	carbon fluxes	729:741	strain PCC 6803 physiology, with a strong impact on the export of EPS and carbon fluxes.
33504656	8	90	theme	structural	1108:1117	arg1	component					1119:1127	the most important structural component	1089:1127	the most important structural component of pili	1089:1135	Furthermore, the most important structural component of pili, the protein PilA, was found to have a modified glycosylation pattern in the mutant compared to the wild type.
33504656	8	90	theme	structural	1108:1117	arg1	PilA					1150:1153	the protein PilA	1138:1153	the protein PilA	1138:1153	Furthermore, the most important structural component of pili, the protein PilA, was found to have a modified glycosylation pattern in the mutant compared to the wild type.
33504656	4	91	theme	putative	563:570	arg1	KpsM					593:596	KpsM	593:596	KpsM (Slr0977)	593:606	Here, we show that the absence of a putative EPS-related protein, KpsM (Slr0977), has a pleiotropic effect on Synechocystis sp.
33504656	4	91	theme	putative	563:570	arg1	protein					584:590	a putative EPS-related protein	561:590	a putative EPS-related protein	561:590	Here, we show that the absence of a putative EPS-related protein, KpsM (Slr0977), has a pleiotropic effect on Synechocystis sp.
33504656	11	92	theme	EPS	2161:2163	arg1	production					2165:2174	EPS production	2161:2174	EPS production	2161:2174	The interest in the cyanobacterial EPS synthesis/export pathways has been increasing, not only to optimize EPS production but also to efficiently redirect carbon flux toward the production of other compounds, allowing the implementation of industrial systems based on cyanobacterial cell factories.
33504656	0	93	theme	Synechocystis	121:133	arg1	sp					135:136	Synechocystis sp	121:136	Synechocystis sp	121:136	Absence of KpsM (Slr0977) Impairs the Secretion of Extracellular Polymeric Substances (EPS) and Impacts Carbon Fluxes in Synechocystis sp.
33504656	9	94	theme	photosynthesis	1378:1391	arg1	mechanisms					1322:1331	the mechanisms	1318:1331	the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism	1318:1441	Proteomic and transcriptomic analyses revealed significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism, in response to the inactivation of slr0977 Overall, this work shows for the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments, highlighting the importance of EPS as a major carbon sink in cyanobacteria.
33504656	6	95	theme	smaller	827:833	arg1	decrease					835:842	a smaller decrease	825:842	a smaller decrease of capsular polysaccharides	825:870	The kpsM mutant exhibits a significant reduction of released polysaccharides and a smaller decrease of capsular polysaccharides, but it accumulates more polyhydroxybutyrate (PHB) than the wild type.
33504656	13	96	gly	glycosylation	2637:2649	arg1	PilA					2654:2657	PilA	2654:2657	PilA	2654:2657	Further characterization showed a light/cell density-dependent clumping phenotype, altered protein secretion, and modified glycosylation of PilA.
33504656	9	97	theme	oxidative	1394:1402	arg1	phosphorylation					1404:1418	oxidative phosphorylation	1394:1418	oxidative phosphorylation	1394:1418	Proteomic and transcriptomic analyses revealed significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism, in response to the inactivation of slr0977 Overall, this work shows for the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments, highlighting the importance of EPS as a major carbon sink in cyanobacteria.
33504656	15	98	theme	Synechocystis	2876:2888	arg1	physiology					2890:2899	Synechocystis physiology	2876:2899	Synechocystis physiology	2876:2899	Altogether, this work provides a comprehensive overview of the impact of kpsM disruption on Synechocystis physiology, highlighting the importance of EPS as a carbon sink and showing how cells adapt when their secretion is impaired, and the redirection of the carbon fluxes.
33504656	8	99	theme	modified	1176:1183	arg1	pattern					1199:1205	a modified glycosylation pattern	1174:1205	a modified glycosylation pattern	1174:1205	Furthermore, the most important structural component of pili, the protein PilA, was found to have a modified glycosylation pattern in the mutant compared to the wild type.
33504656	10	100	theme	Most	1896:1899	arg1	cyanobacteria					1901:1913	interest.IMPORTANCE Most cyanobacteria	1876:1913	interest.IMPORTANCE Most cyanobacteria	1876:1913	The accumulation of PHB in cells of the mutant, without affecting significantly its fitness/growth rate, points to its possible use as a chassis for the production of compounds of interest.IMPORTANCE Most cyanobacteria produce extracellular polymeric substances (EPS) that fulfill different biological roles depending on the strain/environmental conditions.
33504656	13	101	theme	clumping	2577:2584	arg1	phenotype					2586:2594	a light/cell density-dependent clumping phenotype	2546:2594	a light/cell density-dependent clumping phenotype	2546:2594	Further characterization showed a light/cell density-dependent clumping phenotype, altered protein secretion, and modified glycosylation of PilA.
33504656	10	102	theme	PHB	1716:1718	arg1	accumulation					1700:1711	The accumulation	1696:1711	The accumulation of PHB in cells of the mutant	1696:1741	The accumulation of PHB in cells of the mutant, without affecting significantly its fitness/growth rate, points to its possible use as a chassis for the production of compounds of interest.IMPORTANCE Most cyanobacteria produce extracellular polymeric substances (EPS) that fulfill different biological roles depending on the strain/environmental conditions.
33504656	5	103	theme	EPS	721:723	arg1	export					711:716	the export	707:716	the export of EPS and carbon fluxes	707:741	strain PCC 6803 physiology, with a strong impact on the export of EPS and carbon fluxes.
33504656	15	104	from	overview	2831:2838	arg1	physiology					2890:2899	Synechocystis physiology	2876:2899	Synechocystis physiology	2876:2899	Altogether, this work provides a comprehensive overview of the impact of kpsM disruption on Synechocystis physiology, highlighting the importance of EPS as a carbon sink and showing how cells adapt when their secretion is impaired, and the redirection of the carbon fluxes.
33504656	9	105	theme	EPS	1651:1653	arg1	sink					1673:1676	a major carbon sink	1658:1676	a major carbon sink in cyanobacteria	1658:1693	Proteomic and transcriptomic analyses revealed significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism, in response to the inactivation of slr0977 Overall, this work shows for the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments, highlighting the importance of EPS as a major carbon sink in cyanobacteria.
33504656	9	105	theme	EPS	1651:1653	arg1	importance					1637:1646	the importance	1633:1646	the importance of EPS	1633:1653	Proteomic and transcriptomic analyses revealed significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism, in response to the inactivation of slr0977 Overall, this work shows for the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments, highlighting the importance of EPS as a major carbon sink in cyanobacteria.
33504656	15	106	theme	disruption	2862:2871	arg1	impact					2847:2852	the impact	2843:2852	the impact of kpsM disruption on Synechocystis physiology, highlighting the importance of EPS as a carbon sink and showing how cells adapt when their secretion is impaired,	2843:3014	Altogether, this work provides a comprehensive overview of the impact of kpsM disruption on Synechocystis physiology, highlighting the importance of EPS as a carbon sink and showing how cells adapt when their secretion is impaired, and the redirection of the carbon fluxes.
33504656	0	107	theme	Polymeric	65:73	arg1	EPS					87:89	EPS	87:89	EPS	87:89	Absence of KpsM (Slr0977) Impairs the Secretion of Extracellular Polymeric Substances (EPS) and Impacts Carbon Fluxes in Synechocystis sp.
33504656	0	107	theme	Polymeric	65:73	arg1	Substances					75:84	Extracellular Polymeric Substances	51:84	Extracellular Polymeric Substances (EPS)	51:90	Absence of KpsM (Slr0977) Impairs the Secretion of Extracellular Polymeric Substances (EPS) and Impacts Carbon Fluxes in Synechocystis sp.
33504656	5	108	from	impact	697:702	arg1	export					711:716	the export	707:716	the export of EPS and carbon fluxes	707:741	strain PCC 6803 physiology, with a strong impact on the export of EPS and carbon fluxes.
33504656	9	109	theme	carbon	1425:1430	arg1	metabolism					1432:1441	carbon metabolism	1425:1441	carbon metabolism	1425:1441	Proteomic and transcriptomic analyses revealed significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism, in response to the inactivation of slr0977 Overall, this work shows for the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments, highlighting the importance of EPS as a major carbon sink in cyanobacteria.
33504656	11	110	theme	industrial	2294:2303	arg1	systems					2305:2311	industrial systems	2294:2311	industrial systems based on cyanobacterial cell factories	2294:2350	The interest in the cyanobacterial EPS synthesis/export pathways has been increasing, not only to optimize EPS production but also to efficiently redirect carbon flux toward the production of other compounds, allowing the implementation of industrial systems based on cyanobacterial cell factories.
33504656	9	111	dep	inactivation	1463:1474	arg1	response					1447:1454	response	1447:1454	response	1447:1454	Proteomic and transcriptomic analyses revealed significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism, in response to the inactivation of slr0977 Overall, this work shows for the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments, highlighting the importance of EPS as a major carbon sink in cyanobacteria.
33504656	10	112	theme	polymeric	1937:1945	arg1	EPS					1959:1961	EPS	1959:1961	EPS	1959:1961	The accumulation of PHB in cells of the mutant, without affecting significantly its fitness/growth rate, points to its possible use as a chassis for the production of compounds of interest.IMPORTANCE Most cyanobacteria produce extracellular polymeric substances (EPS) that fulfill different biological roles depending on the strain/environmental conditions.
33504656	10	112	theme	polymeric	1937:1945	arg1	substances					1947:1956	extracellular polymeric substances	1923:1956	extracellular polymeric substances (EPS) that fulfill different biological roles depending on the strain/environmental conditions	1923:2051	The accumulation of PHB in cells of the mutant, without affecting significantly its fitness/growth rate, points to its possible use as a chassis for the production of compounds of interest.IMPORTANCE Most cyanobacteria produce extracellular polymeric substances (EPS) that fulfill different biological roles depending on the strain/environmental conditions.
33504656	15	113	theme	fluxes	3050:3055	arg1	overview					2831:2838	a comprehensive overview	2815:2838	a comprehensive overview of the impact of kpsM disruption on Synechocystis physiology, highlighting the importance of EPS as a carbon sink and showing how cells adapt when their secretion is impaired,	2815:3014	Altogether, this work provides a comprehensive overview of the impact of kpsM disruption on Synechocystis physiology, highlighting the importance of EPS as a carbon sink and showing how cells adapt when their secretion is impaired, and the redirection of the carbon fluxes.
33504656	15	113	theme	fluxes	3050:3055	arg1	redirection					3024:3034	the redirection	3020:3034	the redirection of the carbon fluxes	3020:3055	Altogether, this work provides a comprehensive overview of the impact of kpsM disruption on Synechocystis physiology, highlighting the importance of EPS as a carbon sink and showing how cells adapt when their secretion is impaired, and the redirection of the carbon fluxes.
33504656	9	114	theme	carbon	1666:1671	arg1	sink					1673:1676	a major carbon sink	1658:1676	a major carbon sink in cyanobacteria	1658:1693	Proteomic and transcriptomic analyses revealed significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism, in response to the inactivation of slr0977 Overall, this work shows for the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments, highlighting the importance of EPS as a major carbon sink in cyanobacteria.
33504656	9	114	theme	carbon	1666:1671	arg1	importance					1637:1646	the importance	1633:1646	the importance of EPS	1633:1653	Proteomic and transcriptomic analyses revealed significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism, in response to the inactivation of slr0977 Overall, this work shows for the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments, highlighting the importance of EPS as a major carbon sink in cyanobacteria.
33504656	13	115	theme	light/cell	2548:2557	arg1	phenotype					2586:2594	a light/cell density-dependent clumping phenotype	2546:2594	a light/cell density-dependent clumping phenotype	2546:2594	Further characterization showed a light/cell density-dependent clumping phenotype, altered protein secretion, and modified glycosylation of PilA.
33504656	15	116	from	redirection	3024:3034	arg1	physiology					2890:2899	Synechocystis physiology	2876:2899	Synechocystis physiology	2876:2899	Altogether, this work provides a comprehensive overview of the impact of kpsM disruption on Synechocystis physiology, highlighting the importance of EPS as a carbon sink and showing how cells adapt when their secretion is impaired, and the redirection of the carbon fluxes.
33504656	14	117	theme	mutant	2698:2703	arg1	proteome					2664:2671	proteome	2664:2671	proteome	2664:2671	The proteome and transcriptome of the mutant revealed significant changes, namely, in photosynthesis and carbon metabolism.
33504656	14	117	theme	mutant	2698:2703	arg1	transcriptome					2677:2689	transcriptome	2677:2689	transcriptome	2677:2689	The proteome and transcriptome of the mutant revealed significant changes, namely, in photosynthesis and carbon metabolism.
33504656	11	118	theme	cyanobacterial	2322:2335	arg1	factories					2342:2350	cyanobacterial cell factories	2322:2350	cyanobacterial cell factories	2322:2350	The interest in the cyanobacterial EPS synthesis/export pathways has been increasing, not only to optimize EPS production but also to efficiently redirect carbon flux toward the production of other compounds, allowing the implementation of industrial systems based on cyanobacterial cell factories.
33504656	5	119	dep	PCC	662:664	arg1	physiology					671:680	physiology	671:680	physiology	671:680	strain PCC 6803 physiology, with a strong impact on the export of EPS and carbon fluxes.
33504656	4	120	theme	Synechocystis	637:649	arg1	sp					651:652	Synechocystis sp	637:652	Synechocystis sp	637:652	Here, we show that the absence of a putative EPS-related protein, KpsM (Slr0977), has a pleiotropic effect on Synechocystis sp.
33504656	7	121	theme	secretion	1056:1064	arg1	capacity					1066:1073	an altered protein secretion capacity	1037:1073	an altered protein secretion capacity	1037:1073	In addition, this strain shows a light/cell density-dependent clumping phenotype and exhibits an altered protein secretion capacity.
33504656	15	122	theme	EPS	2933:2935	arg1	importance					2919:2928	the importance	2915:2928	the importance of EPS	2915:2935	Altogether, this work provides a comprehensive overview of the impact of kpsM disruption on Synechocystis physiology, highlighting the importance of EPS as a carbon sink and showing how cells adapt when their secretion is impaired, and the redirection of the carbon fluxes.
33504656	15	122	theme	EPS	2933:2935	arg1	sink					2949:2952	a carbon sink	2940:2952	a carbon sink	2940:2952	Altogether, this work provides a comprehensive overview of the impact of kpsM disruption on Synechocystis physiology, highlighting the importance of EPS as a carbon sink and showing how cells adapt when their secretion is impaired, and the redirection of the carbon fluxes.
33504656	2	123	theme	Many	149:152	arg1	cyanobacteria					154:166	Many cyanobacteria	149:166	Many cyanobacteria	149:166	Many cyanobacteria produce extracellular polymeric substances (EPS), composed mainly of heteropolysaccharides, that play a variety of physiological roles, being crucial for cell protection, motility, and biofilm formation.
33504656	3	124	theme	biosynthetic	414:425	arg1	pathways					427:434	the EPS biosynthetic pathways	406:434	the EPS biosynthetic pathways as well as their assembly and export mechanisms	406:482	However, due to their complexity, the EPS biosynthetic pathways as well as their assembly and export mechanisms are still far from being fully understood.
33504656	7	125	theme	altered	1040:1046	arg1	capacity					1066:1073	an altered protein secretion capacity	1037:1073	an altered protein secretion capacity	1037:1073	In addition, this strain shows a light/cell density-dependent clumping phenotype and exhibits an altered protein secretion capacity.
33504656	9	126	from	changes	1307:1313	arg1	mechanisms					1322:1331	the mechanisms	1318:1331	the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism	1318:1441	Proteomic and transcriptomic analyses revealed significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism, in response to the inactivation of slr0977 Overall, this work shows for the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments, highlighting the importance of EPS as a major carbon sink in cyanobacteria.
33504656	6	127	theme	kpsM	748:751	arg1	mutant					753:758	The kpsM mutant	744:758	The kpsM mutant	744:758	The kpsM mutant exhibits a significant reduction of released polysaccharides and a smaller decrease of capsular polysaccharides, but it accumulates more polyhydroxybutyrate (PHB) than the wild type.
33504656	9	128	theme	Overall	1487:1493	arg1	slr0977					1479:1485	slr0977 Overall	1479:1493	slr0977 Overall	1479:1493	Proteomic and transcriptomic analyses revealed significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism, in response to the inactivation of slr0977 Overall, this work shows for the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments, highlighting the importance of EPS as a major carbon sink in cyanobacteria.
33504656	6	129	theme	wild	932:935	arg1	type					937:940	the wild type	928:940	the wild type	928:940	The kpsM mutant exhibits a significant reduction of released polysaccharides and a smaller decrease of capsular polysaccharides, but it accumulates more polyhydroxybutyrate (PHB) than the wild type.
33504656	13	130	theme	density-dependent	2559:2575	arg1	phenotype					2586:2594	a light/cell density-dependent clumping phenotype	2546:2594	a light/cell density-dependent clumping phenotype	2546:2594	Further characterization showed a light/cell density-dependent clumping phenotype, altered protein secretion, and modified glycosylation of PilA.
33504656	5	131	theme	strong	690:695	arg1	impact					697:702	a strong impact	688:702	a strong impact on the export of EPS and carbon fluxes	688:741	strain PCC 6803 physiology, with a strong impact on the export of EPS and carbon fluxes.
33504656	2	132	theme	polymeric	190:198	arg1	EPS					212:214	EPS	212:214	EPS	212:214	Many cyanobacteria produce extracellular polymeric substances (EPS), composed mainly of heteropolysaccharides, that play a variety of physiological roles, being crucial for cell protection, motility, and biofilm formation.
33504656	2	132	theme	polymeric	190:198	arg1	substances					200:209	extracellular polymeric substances	176:209	extracellular polymeric substances (EPS)	176:215	Many cyanobacteria produce extracellular polymeric substances (EPS), composed mainly of heteropolysaccharides, that play a variety of physiological roles, being crucial for cell protection, motility, and biofilm formation.
33504656	12	133	theme	wild	2437:2440	arg1	type					2442:2445	the wild type	2433:2445	the wild type	2433:2445	Here, we show that a Synechocystis kpsM (slr0977) mutant secretes less EPS than the wild type, accumulating more carbon intracellularly, as polyhydroxybutyrate.
33504656	11	134	theme	other	2246:2250	arg1	compounds					2252:2260	other compounds	2246:2260	other compounds	2246:2260	The interest in the cyanobacterial EPS synthesis/export pathways has been increasing, not only to optimize EPS production but also to efficiently redirect carbon flux toward the production of other compounds, allowing the implementation of industrial systems based on cyanobacterial cell factories.
33504656	0	135	from	Fluxes	111:116	arg1	sp					135:136	Synechocystis sp	121:136	Synechocystis sp	121:136	Absence of KpsM (Slr0977) Impairs the Secretion of Extracellular Polymeric Substances (EPS) and Impacts Carbon Fluxes in Synechocystis sp.
33504656	2	136	theme	roles	297:301	arg1	variety					272:278	a variety	270:278	a variety of physiological roles	270:301	Many cyanobacteria produce extracellular polymeric substances (EPS), composed mainly of heteropolysaccharides, that play a variety of physiological roles, being crucial for cell protection, motility, and biofilm formation.
33504656	2	136	theme	roles	297:301	arg1	roles					297:301	physiological roles	283:301	physiological roles	283:301	Many cyanobacteria produce extracellular polymeric substances (EPS), composed mainly of heteropolysaccharides, that play a variety of physiological roles, being crucial for cell protection, motility, and biofilm formation.
33504656	3	137	theme	export	466:471	arg1	mechanisms					473:482	their assembly and export mechanisms	447:482	mechanisms	473:482	However, due to their complexity, the EPS biosynthetic pathways as well as their assembly and export mechanisms are still far from being fully understood.
33504656	9	138	theme	significant	1295:1305	arg1	changes					1307:1313	significant changes	1295:1313	significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism	1295:1441	Proteomic and transcriptomic analyses revealed significant changes in the mechanisms of energy production and conversion, namely, photosynthesis, oxidative phosphorylation, and carbon metabolism, in response to the inactivation of slr0977 Overall, this work shows for the first time that cells with impaired EPS secretion undergo transcriptomic and proteomic adjustments, highlighting the importance of EPS as a major carbon sink in cyanobacteria.
34328161	0	0	theme	hypoglycemic	98:109	arg1	effect					111:116	hypoglycemic effect	98:116	hypoglycemic effect	98:116	Insulin- and cholic acid-loaded zein/casein-dextran nanoparticles enhance the oral absorption and hypoglycemic effect of insulin.
34328161	6	1	theme	loaded	892:897	arg1	insulin					899:905	the loaded insulin	888:905	the loaded insulin	888:905	The dextran significantly increased the disperse stability of the nanoparticles, protected the loaded insulin from hydrolysis in digestive juices, and increased the trans-mucus permeability of the insulin.
34328161	7	2	theme	cholic	1016:1021	arg1	acid					1023:1026	embedded cholic acid	1007:1026	The embedded cholic acid molecules	1003:1036	The embedded cholic acid molecules were consecutively exposed to the surface when the nanoparticles were gradually eroded by proteases.
34328161	1	3	theme	metabolic	167:175	arg1	mellitus					139:146	Diabetes mellitus	130:146	Diabetes mellitus	130:146	Diabetes mellitus is the most common metabolic disease in the world.
34328161	1	3	theme	metabolic	167:175	arg1	disease					177:183	the most common metabolic disease	151:183	the most common metabolic disease in the world	151:196	Diabetes mellitus is the most common metabolic disease in the world.
34328161	7	4	theme	acid	1023:1026	arg1	molecules					1028:1036	The embedded cholic acid molecules	1003:1036	The embedded cholic acid molecules	1003:1036	The embedded cholic acid molecules were consecutively exposed to the surface when the nanoparticles were gradually eroded by proteases.
34328161	9	5	theme	transport	1421:1429	arg1	pathway					1431:1437	the intestinal lymphatic transport pathway	1396:1437	the intestinal lymphatic transport pathway	1396:1437	The effect of pretreated lymphatic transport inhibitor cycloheximide revealed that about half of the nanoparticles were transported via the intestinal lymphatic transport pathway and the other half of the nanoparticles were transported via portal blood absorption.
34328161	11	6	theme	promising	1676:1684	arg1	nanoparticles					1656:1668	nanoparticles	1656:1668	nanoparticles	1656:1668	This study demonstrates that nanoparticles are a promising oral delivery system for insulin.
34328161	11	6	theme	promising	1676:1684	arg1	system					1700:1705	a promising oral delivery system	1674:1705	a promising oral delivery system for insulin	1674:1717	This study demonstrates that nanoparticles are a promising oral delivery system for insulin.
34328161	8	7	from	absorptions	1176:1186	arg1	liver					1226:1230	liver	1226:1230	liver	1226:1230	The exposed cholic acid promoted the absorptions of the nanoparticles in the ileum and liver via bile acid transporters.
34328161	8	7	from	absorptions	1176:1186	arg1	ileum					1216:1220	ileum	1216:1220	ileum	1216:1220	The exposed cholic acid promoted the absorptions of the nanoparticles in the ileum and liver via bile acid transporters.
34328161	3	8	theme	cholic	491:496	arg1	acid					498:501	cholic acid	491:501	cholic acid	491:501	In the nanoparticles, zein acted as cement to embed insulin, cholic acid and casein by hydrophobic interactions.
34328161	9	9	theme	portal	1500:1505	arg1	absorption					1513:1522	portal blood absorption	1500:1522	portal blood absorption	1500:1522	The effect of pretreated lymphatic transport inhibitor cycloheximide revealed that about half of the nanoparticles were transported via the intestinal lymphatic transport pathway and the other half of the nanoparticles were transported via portal blood absorption.
34328161	8	10	theme	cholic	1151:1156	arg1	acid					1158:1161	The exposed cholic acid	1139:1161	The exposed cholic acid	1139:1161	The exposed cholic acid promoted the absorptions of the nanoparticles in the ileum and liver via bile acid transporters.
34328161	9	11	theme	lymphatic	1285:1293	arg1	cycloheximide					1315:1327	pretreated lymphatic transport inhibitor cycloheximide	1274:1327	pretreated lymphatic transport inhibitor cycloheximide	1274:1327	The effect of pretreated lymphatic transport inhibitor cycloheximide revealed that about half of the nanoparticles were transported via the intestinal lymphatic transport pathway and the other half of the nanoparticles were transported via portal blood absorption.
34328161	1	12	from	disease	177:183	arg1	world					192:196	the world	188:196	the world	188:196	Diabetes mellitus is the most common metabolic disease in the world.
34328161	3	13	theme	hydrophobic	517:527	arg1	interactions					529:540	hydrophobic interactions	517:540	hydrophobic interactions	517:540	In the nanoparticles, zein acted as cement to embed insulin, cholic acid and casein by hydrophobic interactions.
34328161	6	14	theme	trans-mucus	962:972	arg1	permeability					974:985	the trans-mucus permeability	958:985	the trans-mucus permeability of the insulin	958:1000	The dextran significantly increased the disperse stability of the nanoparticles, protected the loaded insulin from hydrolysis in digestive juices, and increased the trans-mucus permeability of the insulin.
34328161	8	15	theme	exposed	1143:1149	arg1	acid					1158:1161	The exposed cholic acid	1139:1161	The exposed cholic acid	1139:1161	The exposed cholic acid promoted the absorptions of the nanoparticles in the ileum and liver via bile acid transporters.
34328161	9	16	theme	transport	1295:1303	arg1	cycloheximide					1315:1327	pretreated lymphatic transport inhibitor cycloheximide	1274:1327	pretreated lymphatic transport inhibitor cycloheximide	1274:1327	The effect of pretreated lymphatic transport inhibitor cycloheximide revealed that about half of the nanoparticles were transported via the intestinal lymphatic transport pathway and the other half of the nanoparticles were transported via portal blood absorption.
34328161	5	17	theme	cholic	718:723	arg1	efficiency					738:747	a cholic acid loading efficiency	716:747	a cholic acid loading efficiency of 55.1%	716:756	The nanoparticles had an insulin loading efficiency of 74.6%, a cholic acid loading efficiency of 55.1% and a hydrodynamic diameter of 267 nm.
34328161	3	18	theme	embed	476:480	arg1	insulin					482:488	embed insulin	476:488	embed insulin	476:488	In the nanoparticles, zein acted as cement to embed insulin, cholic acid and casein by hydrophobic interactions.
34328161	8	19	theme	acid	1241:1244	arg1	transporters					1246:1257	bile acid transporters	1236:1257	bile acid transporters	1236:1257	The exposed cholic acid promoted the absorptions of the nanoparticles in the ileum and liver via bile acid transporters.
34328161	2	20	theme	action	394:399	arg1	liver					367:371	the liver	363:371	the liver which is the primary action organ of endogenous insulin	363:427	Herein, insulin- and cholic acid-loaded zein nanoparticles with dextran surfaces were fabricated to enhance the oral absorptions of insulin in the intestine and in the liver which is the primary action organ of endogenous insulin.
34328161	2	20	theme	action	394:399	arg1	organ					401:405	the primary action organ	382:405	the primary action organ of endogenous insulin	382:427	Herein, insulin- and cholic acid-loaded zein nanoparticles with dextran surfaces were fabricated to enhance the oral absorptions of insulin in the intestine and in the liver which is the primary action organ of endogenous insulin.
34328161	0	21	theme	insulin	121:127	arg1	absorption					83:92	oral absorption	78:92	oral absorption	78:92	Insulin- and cholic acid-loaded zein/casein-dextran nanoparticles enhance the oral absorption and hypoglycemic effect of insulin.
34328161	0	21	theme	insulin	121:127	arg1	effect					111:116	hypoglycemic effect	98:116	hypoglycemic effect	98:116	Insulin- and cholic acid-loaded zein/casein-dextran nanoparticles enhance the oral absorption and hypoglycemic effect of insulin.
34328161	9	22	theme	cycloheximide	1315:1327	arg1	effect					1264:1269	The effect	1260:1269	The effect of pretreated lymphatic transport inhibitor cycloheximide	1260:1327	The effect of pretreated lymphatic transport inhibitor cycloheximide revealed that about half of the nanoparticles were transported via the intestinal lymphatic transport pathway and the other half of the nanoparticles were transported via portal blood absorption.
34328161	2	23	theme	primary	386:392	arg1	liver					367:371	the liver	363:371	the liver which is the primary action organ of endogenous insulin	363:427	Herein, insulin- and cholic acid-loaded zein nanoparticles with dextran surfaces were fabricated to enhance the oral absorptions of insulin in the intestine and in the liver which is the primary action organ of endogenous insulin.
34328161	2	23	theme	primary	386:392	arg1	organ					401:405	the primary action organ	382:405	the primary action organ of endogenous insulin	382:427	Herein, insulin- and cholic acid-loaded zein nanoparticles with dextran surfaces were fabricated to enhance the oral absorptions of insulin in the intestine and in the liver which is the primary action organ of endogenous insulin.
34328161	11	24	theme	delivery	1691:1698	arg1	nanoparticles					1656:1668	nanoparticles	1656:1668	nanoparticles	1656:1668	This study demonstrates that nanoparticles are a promising oral delivery system for insulin.
34328161	11	24	theme	delivery	1691:1698	arg1	system					1700:1705	a promising oral delivery system	1674:1705	a promising oral delivery system for insulin	1674:1717	This study demonstrates that nanoparticles are a promising oral delivery system for insulin.
34328161	10	25	theme	diabetic	1597:1604	arg1	mice					1606:1609	type I diabetic mice	1590:1609	type I diabetic mice	1590:1609	The oral pharmacological bioavailability of the nanoparticles in type I diabetic mice was 12.5-20.5%.
34328161	2	26	theme	insulin	331:337	arg1	absorptions					316:326	the oral absorptions	307:326	the oral absorptions of insulin in the intestine and in the liver which is the primary action organ of endogenous insulin	307:427	Herein, insulin- and cholic acid-loaded zein nanoparticles with dextran surfaces were fabricated to enhance the oral absorptions of insulin in the intestine and in the liver which is the primary action organ of endogenous insulin.
34328161	6	27	theme	nanoparticles	863:875	arg1	stability					846:854	the disperse stability	833:854	the disperse stability of the nanoparticles	833:875	The dextran significantly increased the disperse stability of the nanoparticles, protected the loaded insulin from hydrolysis in digestive juices, and increased the trans-mucus permeability of the insulin.
34328161	8	28	theme	bile	1236:1239	arg1	transporters					1246:1257	bile acid transporters	1236:1257	bile acid transporters	1236:1257	The exposed cholic acid promoted the absorptions of the nanoparticles in the ileum and liver via bile acid transporters.
34328161	0	29	theme	Insulin-	0:7	arg1	nanoparticles					52:64	Insulin- and cholic acid-loaded zein/casein-dextran nanoparticles	0:64	Insulin- and cholic acid-loaded zein/casein-dextran nanoparticles	0:64	Insulin- and cholic acid-loaded zein/casein-dextran nanoparticles enhance the oral absorption and hypoglycemic effect of insulin.
34328161	0	30	theme	acid-loaded	20:30	arg1	nanoparticles					52:64	Insulin- and cholic acid-loaded zein/casein-dextran nanoparticles	0:64	Insulin- and cholic acid-loaded zein/casein-dextran nanoparticles	0:64	Insulin- and cholic acid-loaded zein/casein-dextran nanoparticles enhance the oral absorption and hypoglycemic effect of insulin.
34328161	9	31	theme	other	1447:1451	arg1	half					1453:1456	the other half	1443:1456	the other half of the nanoparticles	1443:1477	The effect of pretreated lymphatic transport inhibitor cycloheximide revealed that about half of the nanoparticles were transported via the intestinal lymphatic transport pathway and the other half of the nanoparticles were transported via portal blood absorption.
34328161	0	32	dep	absorption	83:92	arg1	the					74:76	the	74:76	the	74:76	Insulin- and cholic acid-loaded zein/casein-dextran nanoparticles enhance the oral absorption and hypoglycemic effect of insulin.
34328161	2	33	theme	insulin	421:427	arg1	liver					367:371	the liver	363:371	the liver which is the primary action organ of endogenous insulin	363:427	Herein, insulin- and cholic acid-loaded zein nanoparticles with dextran surfaces were fabricated to enhance the oral absorptions of insulin in the intestine and in the liver which is the primary action organ of endogenous insulin.
34328161	2	33	theme	insulin	421:427	arg1	organ					401:405	the primary action organ	382:405	the primary action organ of endogenous insulin	382:427	Herein, insulin- and cholic acid-loaded zein nanoparticles with dextran surfaces were fabricated to enhance the oral absorptions of insulin in the intestine and in the liver which is the primary action organ of endogenous insulin.
34328161	0	34	theme	cholic	13:18	arg1	nanoparticles					52:64	Insulin- and cholic acid-loaded zein/casein-dextran nanoparticles	0:64	Insulin- and cholic acid-loaded zein/casein-dextran nanoparticles	0:64	Insulin- and cholic acid-loaded zein/casein-dextran nanoparticles enhance the oral absorption and hypoglycemic effect of insulin.
34328161	2	35	with	nanoparticles	244:256	arg1	surfaces					271:278	dextran surfaces	263:278	dextran surfaces	263:278	Herein, insulin- and cholic acid-loaded zein nanoparticles with dextran surfaces were fabricated to enhance the oral absorptions of insulin in the intestine and in the liver which is the primary action organ of endogenous insulin.
34328161	2	36	theme	oral	311:314	arg1	absorptions					316:326	the oral absorptions	307:326	the oral absorptions of insulin in the intestine and in the liver which is the primary action organ of endogenous insulin	307:427	Herein, insulin- and cholic acid-loaded zein nanoparticles with dextran surfaces were fabricated to enhance the oral absorptions of insulin in the intestine and in the liver which is the primary action organ of endogenous insulin.
34328161	2	37	theme	endogenous	410:419	arg1	insulin					421:427	endogenous insulin	410:427	endogenous insulin	410:427	Herein, insulin- and cholic acid-loaded zein nanoparticles with dextran surfaces were fabricated to enhance the oral absorptions of insulin in the intestine and in the liver which is the primary action organ of endogenous insulin.
34328161	5	38	theme	%	756:756	arg1	efficiency					738:747	a cholic acid loading efficiency	716:747	a cholic acid loading efficiency of 55.1%	716:756	The nanoparticles had an insulin loading efficiency of 74.6%, a cholic acid loading efficiency of 55.1% and a hydrodynamic diameter of 267 nm.
34328161	5	38	theme	%	756:756	arg1	diameter					777:784	a hydrodynamic diameter	762:784	a hydrodynamic diameter of 267 nm	762:794	The nanoparticles had an insulin loading efficiency of 74.6%, a cholic acid loading efficiency of 55.1% and a hydrodynamic diameter of 267 nm.
34328161	5	38	theme	%	756:756	arg1	efficiency					695:704	an insulin loading efficiency	676:704	an insulin loading efficiency of 74.6%	676:713	The nanoparticles had an insulin loading efficiency of 74.6%, a cholic acid loading efficiency of 55.1% and a hydrodynamic diameter of 267 nm.
34328161	4	39	theme	hydrophilic	547:557	arg1	located					617:623	located	617:623	located	617:623	The hydrophilic dextran conjugated to casein by the Maillard reaction was located on the nanoparticle surface.
34328161	4	39	theme	hydrophilic	547:557	arg1	dextran					559:565	The hydrophilic dextran	543:565	The hydrophilic dextran conjugated to casein by the Maillard reaction	543:611	The hydrophilic dextran conjugated to casein by the Maillard reaction was located on the nanoparticle surface.
34328161	9	40	theme	inhibitor	1305:1313	arg1	cycloheximide					1315:1327	pretreated lymphatic transport inhibitor cycloheximide	1274:1327	pretreated lymphatic transport inhibitor cycloheximide	1274:1327	The effect of pretreated lymphatic transport inhibitor cycloheximide revealed that about half of the nanoparticles were transported via the intestinal lymphatic transport pathway and the other half of the nanoparticles were transported via portal blood absorption.
34328161	6	41	theme	disperse	837:844	arg1	stability					846:854	the disperse stability	833:854	the disperse stability of the nanoparticles	833:875	The dextran significantly increased the disperse stability of the nanoparticles, protected the loaded insulin from hydrolysis in digestive juices, and increased the trans-mucus permeability of the insulin.
34328161	10	42	from	bioavailability	1550:1564	arg1	mice					1606:1609	type I diabetic mice	1590:1609	type I diabetic mice	1590:1609	The oral pharmacological bioavailability of the nanoparticles in type I diabetic mice was 12.5-20.5%.
34328161	4	43	theme	nanoparticle	632:643	arg1	surface					645:651	the nanoparticle surface	628:651	the nanoparticle surface	628:651	The hydrophilic dextran conjugated to casein by the Maillard reaction was located on the nanoparticle surface.
34328161	0	44	theme	zein/casein-dextran	32:50	arg1	nanoparticles					52:64	Insulin- and cholic acid-loaded zein/casein-dextran nanoparticles	0:64	Insulin- and cholic acid-loaded zein/casein-dextran nanoparticles	0:64	Insulin- and cholic acid-loaded zein/casein-dextran nanoparticles enhance the oral absorption and hypoglycemic effect of insulin.
34328161	6	45	theme	digestive	926:934	arg1	juices					936:941	digestive juices	926:941	digestive juices	926:941	The dextran significantly increased the disperse stability of the nanoparticles, protected the loaded insulin from hydrolysis in digestive juices, and increased the trans-mucus permeability of the insulin.
34328161	5	46	theme	%	713:713	arg1	efficiency					738:747	a cholic acid loading efficiency	716:747	a cholic acid loading efficiency of 55.1%	716:756	The nanoparticles had an insulin loading efficiency of 74.6%, a cholic acid loading efficiency of 55.1% and a hydrodynamic diameter of 267 nm.
34328161	5	46	theme	%	713:713	arg1	diameter					777:784	a hydrodynamic diameter	762:784	a hydrodynamic diameter of 267 nm	762:794	The nanoparticles had an insulin loading efficiency of 74.6%, a cholic acid loading efficiency of 55.1% and a hydrodynamic diameter of 267 nm.
34328161	5	46	theme	%	713:713	arg1	efficiency					695:704	an insulin loading efficiency	676:704	an insulin loading efficiency of 74.6%	676:713	The nanoparticles had an insulin loading efficiency of 74.6%, a cholic acid loading efficiency of 55.1% and a hydrodynamic diameter of 267 nm.
34328161	2	47	theme	zein	239:242	arg1	nanoparticles					244:256	insulin- and cholic acid-loaded zein nanoparticles	207:256	insulin- and cholic acid-loaded zein nanoparticles with dextran surfaces	207:278	Herein, insulin- and cholic acid-loaded zein nanoparticles with dextran surfaces were fabricated to enhance the oral absorptions of insulin in the intestine and in the liver which is the primary action organ of endogenous insulin.
34328161	5	48	theme	acid	725:728	arg1	efficiency					738:747	a cholic acid loading efficiency	716:747	a cholic acid loading efficiency of 55.1%	716:756	The nanoparticles had an insulin loading efficiency of 74.6%, a cholic acid loading efficiency of 55.1% and a hydrodynamic diameter of 267 nm.
34328161	9	49	theme	nanoparticles	1361:1373	arg1	half					1349:1352	about half	1343:1352	about half of the nanoparticles	1343:1373	The effect of pretreated lymphatic transport inhibitor cycloheximide revealed that about half of the nanoparticles were transported via the intestinal lymphatic transport pathway and the other half of the nanoparticles were transported via portal blood absorption.
34328161	6	50	from	hydrolysis	912:921	arg1	juices					936:941	digestive juices	926:941	digestive juices	926:941	The dextran significantly increased the disperse stability of the nanoparticles, protected the loaded insulin from hydrolysis in digestive juices, and increased the trans-mucus permeability of the insulin.
34328161	10	51	theme	nanoparticles	1573:1585	arg1	%					1624:1624	12.5-20.5%	1615:1624	12.5-20.5%	1615:1624	The oral pharmacological bioavailability of the nanoparticles in type I diabetic mice was 12.5-20.5%.
34328161	10	51	theme	nanoparticles	1573:1585	arg1	bioavailability					1550:1564	The oral pharmacological bioavailability	1525:1564	The oral pharmacological bioavailability of the nanoparticles in type I diabetic mice	1525:1609	The oral pharmacological bioavailability of the nanoparticles in type I diabetic mice was 12.5-20.5%.
34328161	2	52	theme	acid-loaded	227:237	arg1	zein					239:242	cholic acid-loaded zein	220:242	cholic acid-loaded zein	220:242	Herein, insulin- and cholic acid-loaded zein nanoparticles with dextran surfaces were fabricated to enhance the oral absorptions of insulin in the intestine and in the liver which is the primary action organ of endogenous insulin.
34328161	7	53	theme	embedded	1007:1014	arg1	acid					1023:1026	embedded cholic acid	1007:1026	The embedded cholic acid molecules	1003:1036	The embedded cholic acid molecules were consecutively exposed to the surface when the nanoparticles were gradually eroded by proteases.
34328161	1	54	theme	Diabetes	130:137	arg1	mellitus					139:146	Diabetes mellitus	130:146	Diabetes mellitus	130:146	Diabetes mellitus is the most common metabolic disease in the world.
34328161	1	54	theme	Diabetes	130:137	arg1	disease					177:183	the most common metabolic disease	151:183	the most common metabolic disease in the world	151:196	Diabetes mellitus is the most common metabolic disease in the world.
34328161	5	55	theme	hydrodynamic	764:775	arg1	diameter					777:784	a hydrodynamic diameter	762:784	a hydrodynamic diameter of 267 nm	762:794	The nanoparticles had an insulin loading efficiency of 74.6%, a cholic acid loading efficiency of 55.1% and a hydrodynamic diameter of 267 nm.
34328161	10	56	theme	pharmacological	1534:1548	arg1	%					1624:1624	12.5-20.5%	1615:1624	12.5-20.5%	1615:1624	The oral pharmacological bioavailability of the nanoparticles in type I diabetic mice was 12.5-20.5%.
34328161	10	56	theme	pharmacological	1534:1548	arg1	bioavailability					1550:1564	The oral pharmacological bioavailability	1525:1564	The oral pharmacological bioavailability of the nanoparticles in type I diabetic mice	1525:1609	The oral pharmacological bioavailability of the nanoparticles in type I diabetic mice was 12.5-20.5%.
34328161	4	57	located	located	617:623	arg2	dextran					559:565	The hydrophilic dextran	543:565	The hydrophilic dextran conjugated to casein by the Maillard reaction	543:611	The hydrophilic dextran conjugated to casein by the Maillard reaction was located on the nanoparticle surface.
34328161	4	57	located	located	617:623	arg2	located					617:623	located	617:623	located	617:623	The hydrophilic dextran conjugated to casein by the Maillard reaction was located on the nanoparticle surface.
34328161	4	57	located	located	617:623	arg1	surface					645:651	the nanoparticle surface	628:651	the nanoparticle surface	628:651	The hydrophilic dextran conjugated to casein by the Maillard reaction was located on the nanoparticle surface.
34328161	2	58	theme	cholic	220:225	arg1	zein					239:242	cholic acid-loaded zein	220:242	cholic acid-loaded zein	220:242	Herein, insulin- and cholic acid-loaded zein nanoparticles with dextran surfaces were fabricated to enhance the oral absorptions of insulin in the intestine and in the liver which is the primary action organ of endogenous insulin.
34328161	9	59	theme	blood	1507:1511	arg1	absorption					1513:1522	portal blood absorption	1500:1522	portal blood absorption	1500:1522	The effect of pretreated lymphatic transport inhibitor cycloheximide revealed that about half of the nanoparticles were transported via the intestinal lymphatic transport pathway and the other half of the nanoparticles were transported via portal blood absorption.
34328161	5	60	contain	had	672:674	arg2	diameter					777:784	a hydrodynamic diameter	762:784	a hydrodynamic diameter of 267 nm	762:794	The nanoparticles had an insulin loading efficiency of 74.6%, a cholic acid loading efficiency of 55.1% and a hydrodynamic diameter of 267 nm.
34328161	5	60	contain	had	672:674	arg2	efficiency					738:747	a cholic acid loading efficiency	716:747	a cholic acid loading efficiency of 55.1%	716:756	The nanoparticles had an insulin loading efficiency of 74.6%, a cholic acid loading efficiency of 55.1% and a hydrodynamic diameter of 267 nm.
34328161	5	60	contain	had	672:674	arg2	efficiency					695:704	an insulin loading efficiency	676:704	an insulin loading efficiency of 74.6%	676:713	The nanoparticles had an insulin loading efficiency of 74.6%, a cholic acid loading efficiency of 55.1% and a hydrodynamic diameter of 267 nm.
34328161	5	60	contain	had	672:674	arg1	nanoparticles					658:670	The nanoparticles	654:670	The nanoparticles	654:670	The nanoparticles had an insulin loading efficiency of 74.6%, a cholic acid loading efficiency of 55.1% and a hydrodynamic diameter of 267 nm.
34328161	5	61	theme	insulin	679:685	arg1	efficiency					695:704	an insulin loading efficiency	676:704	an insulin loading efficiency of 74.6%	676:713	The nanoparticles had an insulin loading efficiency of 74.6%, a cholic acid loading efficiency of 55.1% and a hydrodynamic diameter of 267 nm.
34328161	2	62	theme	insulin-	207:214	arg1	nanoparticles					244:256	insulin- and cholic acid-loaded zein nanoparticles	207:256	insulin- and cholic acid-loaded zein nanoparticles with dextran surfaces	207:278	Herein, insulin- and cholic acid-loaded zein nanoparticles with dextran surfaces were fabricated to enhance the oral absorptions of insulin in the intestine and in the liver which is the primary action organ of endogenous insulin.
34328161	11	63	theme	oral	1686:1689	arg1	nanoparticles					1656:1668	nanoparticles	1656:1668	nanoparticles	1656:1668	This study demonstrates that nanoparticles are a promising oral delivery system for insulin.
34328161	11	63	theme	oral	1686:1689	arg1	system					1700:1705	a promising oral delivery system	1674:1705	a promising oral delivery system for insulin	1674:1717	This study demonstrates that nanoparticles are a promising oral delivery system for insulin.
34328161	4	64	theme	Maillard	595:602	arg1	reaction					604:611	the Maillard reaction	591:611	the Maillard reaction	591:611	The hydrophilic dextran conjugated to casein by the Maillard reaction was located on the nanoparticle surface.
34328161	5	65	theme	loading	687:693	arg1	efficiency					695:704	an insulin loading efficiency	676:704	an insulin loading efficiency of 74.6%	676:713	The nanoparticles had an insulin loading efficiency of 74.6%, a cholic acid loading efficiency of 55.1% and a hydrodynamic diameter of 267 nm.
34328161	9	66	theme	intestinal	1400:1409	arg1	pathway					1431:1437	the intestinal lymphatic transport pathway	1396:1437	the intestinal lymphatic transport pathway	1396:1437	The effect of pretreated lymphatic transport inhibitor cycloheximide revealed that about half of the nanoparticles were transported via the intestinal lymphatic transport pathway and the other half of the nanoparticles were transported via portal blood absorption.
34328161	10	67	theme	oral	1529:1532	arg1	%					1624:1624	12.5-20.5%	1615:1624	12.5-20.5%	1615:1624	The oral pharmacological bioavailability of the nanoparticles in type I diabetic mice was 12.5-20.5%.
34328161	10	67	theme	oral	1529:1532	arg1	bioavailability					1550:1564	The oral pharmacological bioavailability	1525:1564	The oral pharmacological bioavailability of the nanoparticles in type I diabetic mice	1525:1609	The oral pharmacological bioavailability of the nanoparticles in type I diabetic mice was 12.5-20.5%.
34328161	8	68	theme	nanoparticles	1195:1207	arg1	absorptions					1176:1186	the absorptions	1172:1186	the absorptions of the nanoparticles in the ileum and liver via bile acid transporters	1172:1257	The exposed cholic acid promoted the absorptions of the nanoparticles in the ileum and liver via bile acid transporters.
34328161	5	69	theme	loading	730:736	arg1	efficiency					738:747	a cholic acid loading efficiency	716:747	a cholic acid loading efficiency of 55.1%	716:756	The nanoparticles had an insulin loading efficiency of 74.6%, a cholic acid loading efficiency of 55.1% and a hydrodynamic diameter of 267 nm.
34328161	2	70	theme	dextran	263:269	arg1	surfaces					271:278	dextran surfaces	263:278	dextran surfaces	263:278	Herein, insulin- and cholic acid-loaded zein nanoparticles with dextran surfaces were fabricated to enhance the oral absorptions of insulin in the intestine and in the liver which is the primary action organ of endogenous insulin.
34328161	9	71	theme	nanoparticles	1465:1477	arg1	half					1453:1456	the other half	1443:1456	the other half of the nanoparticles	1443:1477	The effect of pretreated lymphatic transport inhibitor cycloheximide revealed that about half of the nanoparticles were transported via the intestinal lymphatic transport pathway and the other half of the nanoparticles were transported via portal blood absorption.
34328161	10	72	theme	I	1595:1595	arg1	mice					1606:1609	type I diabetic mice	1590:1609	type I diabetic mice	1590:1609	The oral pharmacological bioavailability of the nanoparticles in type I diabetic mice was 12.5-20.5%.
34328161	6	73	theme	insulin	994:1000	arg1	permeability					974:985	the trans-mucus permeability	958:985	the trans-mucus permeability of the insulin	958:1000	The dextran significantly increased the disperse stability of the nanoparticles, protected the loaded insulin from hydrolysis in digestive juices, and increased the trans-mucus permeability of the insulin.
34328161	0	74	theme	oral	78:81	arg1	absorption					83:92	oral absorption	78:92	oral absorption	78:92	Insulin- and cholic acid-loaded zein/casein-dextran nanoparticles enhance the oral absorption and hypoglycemic effect of insulin.
34328161	9	75	theme	pretreated	1274:1283	arg1	cycloheximide					1315:1327	pretreated lymphatic transport inhibitor cycloheximide	1274:1327	pretreated lymphatic transport inhibitor cycloheximide	1274:1327	The effect of pretreated lymphatic transport inhibitor cycloheximide revealed that about half of the nanoparticles were transported via the intestinal lymphatic transport pathway and the other half of the nanoparticles were transported via portal blood absorption.
34328161	5	76	theme	nm	793:794	arg1	efficiency					738:747	a cholic acid loading efficiency	716:747	a cholic acid loading efficiency of 55.1%	716:756	The nanoparticles had an insulin loading efficiency of 74.6%, a cholic acid loading efficiency of 55.1% and a hydrodynamic diameter of 267 nm.
34328161	5	76	theme	nm	793:794	arg1	diameter					777:784	a hydrodynamic diameter	762:784	a hydrodynamic diameter of 267 nm	762:794	The nanoparticles had an insulin loading efficiency of 74.6%, a cholic acid loading efficiency of 55.1% and a hydrodynamic diameter of 267 nm.
34328161	5	76	theme	nm	793:794	arg1	efficiency					695:704	an insulin loading efficiency	676:704	an insulin loading efficiency of 74.6%	676:713	The nanoparticles had an insulin loading efficiency of 74.6%, a cholic acid loading efficiency of 55.1% and a hydrodynamic diameter of 267 nm.
34328161	10	77	theme	type	1590:1593	arg1	mice					1606:1609	type I diabetic mice	1590:1609	type I diabetic mice	1590:1609	The oral pharmacological bioavailability of the nanoparticles in type I diabetic mice was 12.5-20.5%.
34328161	1	78	theme	common	160:165	arg1	mellitus					139:146	Diabetes mellitus	130:146	Diabetes mellitus	130:146	Diabetes mellitus is the most common metabolic disease in the world.
34328161	1	78	theme	common	160:165	arg1	disease					177:183	the most common metabolic disease	151:183	the most common metabolic disease in the world	151:196	Diabetes mellitus is the most common metabolic disease in the world.
34328161	2	79	from	absorptions	316:326	arg1	intestine					346:354	the intestine	342:354	the intestine	342:354	Herein, insulin- and cholic acid-loaded zein nanoparticles with dextran surfaces were fabricated to enhance the oral absorptions of insulin in the intestine and in the liver which is the primary action organ of endogenous insulin.
34328161	2	79	from	absorptions	316:326	arg1	liver					367:371	the liver	363:371	the liver which is the primary action organ of endogenous insulin	363:427	Herein, insulin- and cholic acid-loaded zein nanoparticles with dextran surfaces were fabricated to enhance the oral absorptions of insulin in the intestine and in the liver which is the primary action organ of endogenous insulin.
34328161	2	79	from	absorptions	316:326	arg1	organ					401:405	the primary action organ	382:405	the primary action organ of endogenous insulin	382:427	Herein, insulin- and cholic acid-loaded zein nanoparticles with dextran surfaces were fabricated to enhance the oral absorptions of insulin in the intestine and in the liver which is the primary action organ of endogenous insulin.
34328161	9	80	theme	lymphatic	1411:1419	arg1	pathway					1431:1437	the intestinal lymphatic transport pathway	1396:1437	the intestinal lymphatic transport pathway	1396:1437	The effect of pretreated lymphatic transport inhibitor cycloheximide revealed that about half of the nanoparticles were transported via the intestinal lymphatic transport pathway and the other half of the nanoparticles were transported via portal blood absorption.
33200762	2	0	theme	beneficial	341:350	arg1	effects					359:365	beneficial health effects	341:365	beneficial health effects due in part to the type of protein, resistant starches and polyphenols	341:436	There is evidence that its consumption generates beneficial health effects due in part to the type of protein, resistant starches and polyphenols.
33200762	4	1	theme	%	786:786	arg1	sucrose					788:794	5% sucrose	785:794	5% sucrose in the drinking water	785:816	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	7	2	from	capacity	1104:1111	arg1	expenditure					1084:1094	energy expenditure	1077:1094	energy expenditure	1077:1094	This change was associated with a significant increase in energy expenditure and the capacity to adapt fuel oxidation to fuel availability.
33200762	4	3	theme	concentrate	611:621	arg1	consumption					581:591	the consumption	577:591	the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB)	577:663	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	3	4	from	use	451:453	arg1	formulation					463:473	food formulation	458:473	food formulation	458:473	Thus, their use in food formulation could impact health status.
33200762	4	5	theme	high-fat	765:772	arg1	diets					774:778	high-fat diets	765:778	high-fat diets with 5% sucrose in the drinking water	765:816	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	3	6	theme	health	488:493	arg1	status					495:500	health status	488:500	health status	488:500	Thus, their use in food formulation could impact health status.
33200762	6	7	theme	high-fat	1004:1011	arg1	diet					1013:1016	a high-fat diet	1002:1016	a high-fat diet	1002:1016	The results showed that consumption of either BPC or WCB reduced weight gain and body fat despite the consumption of a high-fat diet.
33200762	10	8	with	subjects	1651:1658	arg1	resistance					1673:1682	insulin resistance	1665:1682	insulin resistance	1665:1682	The addition of legumes to the diet of subjects with insulin resistance, including black beans, could improve their metabolic status.
33200762	4	9	theme	bean	598:601	arg1	concentrate					611:621	a bean protein concentrate	596:621	a bean protein concentrate (BPC)	596:627	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	4	9	theme	bean	598:601	arg1	BPC					624:626	BPC	624:626	BPC	624:626	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	9	10	attach	present	1483:1489	arg2	compounds					1427:1435	bioactive compounds	1417:1435	bioactive compounds particularly phenolic	1417:1457	These results suggest that the type of protein and bioactive compounds particularly phenolic and flavonoid compounds present in BPC are suitable to improve the formulations used in dietary strategies for subjects with obesity or type 2 diabetes.
33200762	9	10	attach	present	1483:1489	arg1	BPC					1494:1496	BPC	1494:1496	BPC	1494:1496	These results suggest that the type of protein and bioactive compounds particularly phenolic and flavonoid compounds present in BPC are suitable to improve the formulations used in dietary strategies for subjects with obesity or type 2 diabetes.
33200762	10	11	theme	subjects	1651:1658	arg1	diet					1643:1646	the diet	1639:1646	the diet	1639:1646	The addition of legumes to the diet of subjects with insulin resistance, including black beans, could improve their metabolic status.
33200762	7	12	from	increase	1065:1072	arg1	expenditure					1084:1094	energy expenditure	1077:1094	energy expenditure	1077:1094	This change was associated with a significant increase in energy expenditure and the capacity to adapt fuel oxidation to fuel availability.
33200762	4	13	theme	drinking	803:810	arg1	water					812:816	the drinking water	799:816	the drinking water	799:816	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	4	14	theme	bean	648:651	arg1	WCB					660:662	WCB	660:662	WCB	660:662	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	4	14	theme	bean	648:651	arg1	flour					653:657	a whole cooked bean flour	633:657	a whole cooked bean flour (WCB)	633:663	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	0	15	theme	fat-sucrose	142:152	arg1	diet					154:157	a high fat-sucrose diet	135:157	a high fat-sucrose diet	135:157	Black bean protein concentrate ameliorates hepatic steatosis by decreasing lipogenesis and increasing fatty acid oxidation in rats fed a high fat-sucrose diet.
33200762	9	16	theme	type	1595:1598	arg1	diabetes					1602:1609	type 2 diabetes	1595:1609	type 2 diabetes	1595:1609	These results suggest that the type of protein and bioactive compounds particularly phenolic and flavonoid compounds present in BPC are suitable to improve the formulations used in dietary strategies for subjects with obesity or type 2 diabetes.
33200762	0	17	from	oxidation	113:121	arg1	rats					126:129	rats	126:129	rats fed a high fat-sucrose diet	126:157	Black bean protein concentrate ameliorates hepatic steatosis by decreasing lipogenesis and increasing fatty acid oxidation in rats fed a high fat-sucrose diet.
33200762	8	18	theme	lipogenic	1336:1344	arg1	genes					1346:1350	lipogenic genes	1336:1350	lipogenic genes	1336:1350	As a result, rats fed a bean-based diet had lower circulating glucose and insulin concentrations and normal glucose tolerance, which was associated with decreased expression of lipogenic genes in the liver.
33200762	10	19	theme	insulin	1665:1671	arg1	resistance					1673:1682	insulin resistance	1665:1682	insulin resistance	1665:1682	The addition of legumes to the diet of subjects with insulin resistance, including black beans, could improve their metabolic status.
33200762	4	20	theme	whole	635:639	arg1	WCB					660:662	WCB	660:662	WCB	660:662	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	4	20	theme	whole	635:639	arg1	flour					653:657	a whole cooked bean flour	633:657	a whole cooked bean flour (WCB)	633:663	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	7	21	theme	energy	1077:1082	arg1	expenditure					1084:1094	energy expenditure	1077:1094	energy expenditure	1077:1094	This change was associated with a significant increase in energy expenditure and the capacity to adapt fuel oxidation to fuel availability.
33200762	4	22	from	metabolism	694:703	arg1	rats					738:741	Wistar rats	731:741	Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water	731:816	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	4	23	with	diet	757:760	arg1	sucrose					788:794	5% sucrose	785:794	5% sucrose in the drinking water	785:816	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	8	24	theme	bean-based	1183:1192	arg1	diet					1194:1197	a bean-based diet	1181:1197	a bean-based diet	1181:1197	As a result, rats fed a bean-based diet had lower circulating glucose and insulin concentrations and normal glucose tolerance, which was associated with decreased expression of lipogenic genes in the liver.
33200762	0	25	theme	fatty	102:106	arg1	oxidation					113:121	fatty acid oxidation	102:121	fatty acid oxidation in rats fed a high fat-sucrose diet	102:157	Black bean protein concentrate ameliorates hepatic steatosis by decreasing lipogenesis and increasing fatty acid oxidation in rats fed a high fat-sucrose diet.
33200762	9	26	from	BPC	1494:1496	arg1	present					1483:1489	present	1483:1489	present	1483:1489	These results suggest that the type of protein and bioactive compounds particularly phenolic and flavonoid compounds present in BPC are suitable to improve the formulations used in dietary strategies for subjects with obesity or type 2 diabetes.
33200762	8	27	from	expression	1322:1331	arg1	liver					1359:1363	the liver	1355:1363	the liver	1355:1363	As a result, rats fed a bean-based diet had lower circulating glucose and insulin concentrations and normal glucose tolerance, which was associated with decreased expression of lipogenic genes in the liver.
33200762	9	28	theme	protein	1405:1411	arg1	suitable					1502:1509	suitable	1502:1509	suitable	1502:1509	These results suggest that the type of protein and bioactive compounds particularly phenolic and flavonoid compounds present in BPC are suitable to improve the formulations used in dietary strategies for subjects with obesity or type 2 diabetes.
33200762	9	28	theme	protein	1405:1411	arg1	compounds					1473:1481	flavonoid compounds	1463:1481	flavonoid compounds	1463:1481	These results suggest that the type of protein and bioactive compounds particularly phenolic and flavonoid compounds present in BPC are suitable to improve the formulations used in dietary strategies for subjects with obesity or type 2 diabetes.
33200762	9	28	theme	protein	1405:1411	arg1	compounds					1427:1435	bioactive compounds	1417:1435	bioactive compounds particularly phenolic	1417:1457	These results suggest that the type of protein and bioactive compounds particularly phenolic and flavonoid compounds present in BPC are suitable to improve the formulations used in dietary strategies for subjects with obesity or type 2 diabetes.
33200762	9	28	theme	protein	1405:1411	arg1	type					1397:1400	the type	1393:1400	the type of protein	1393:1411	These results suggest that the type of protein and bioactive compounds particularly phenolic and flavonoid compounds present in BPC are suitable to improve the formulations used in dietary strategies for subjects with obesity or type 2 diabetes.
33200762	9	29	from	present	1483:1489	arg1	BPC					1494:1496	BPC	1494:1496	BPC	1494:1496	These results suggest that the type of protein and bioactive compounds particularly phenolic and flavonoid compounds present in BPC are suitable to improve the formulations used in dietary strategies for subjects with obesity or type 2 diabetes.
33200762	2	30	theme	polyphenols	426:436	arg1	type					386:389	the type	382:389	the type of protein, resistant starches and polyphenols	382:436	There is evidence that its consumption generates beneficial health effects due in part to the type of protein, resistant starches and polyphenols.
33200762	8	31	theme	circulating	1209:1219	arg1	glucose					1221:1227	lower circulating glucose	1203:1227	lower circulating glucose	1203:1227	As a result, rats fed a bean-based diet had lower circulating glucose and insulin concentrations and normal glucose tolerance, which was associated with decreased expression of lipogenic genes in the liver.
33200762	0	32	theme	Black	0:4	arg1	concentrate					19:29	Black bean protein concentrate	0:29	Black bean protein concentrate	0:29	Black bean protein concentrate ameliorates hepatic steatosis by decreasing lipogenesis and increasing fatty acid oxidation in rats fed a high fat-sucrose diet.
33200762	9	33	theme	bioactive	1417:1425	arg1	compounds					1427:1435	bioactive compounds	1417:1435	bioactive compounds particularly phenolic	1417:1457	These results suggest that the type of protein and bioactive compounds particularly phenolic and flavonoid compounds present in BPC are suitable to improve the formulations used in dietary strategies for subjects with obesity or type 2 diabetes.
33200762	2	34	theme	starches	413:420	arg1	type					386:389	the type	382:389	the type of protein, resistant starches and polyphenols	382:436	There is evidence that its consumption generates beneficial health effects due in part to the type of protein, resistant starches and polyphenols.
33200762	4	35	theme	body	668:671	arg1	composition					673:683	body composition	668:683	body composition	668:683	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	9	36	with	subjects	1570:1577	arg1	diabetes					1602:1609	type 2 diabetes	1595:1609	type 2 diabetes	1595:1609	These results suggest that the type of protein and bioactive compounds particularly phenolic and flavonoid compounds present in BPC are suitable to improve the formulations used in dietary strategies for subjects with obesity or type 2 diabetes.
33200762	9	36	with	subjects	1570:1577	arg1	obesity					1584:1590	obesity	1584:1590	obesity	1584:1590	These results suggest that the type of protein and bioactive compounds particularly phenolic and flavonoid compounds present in BPC are suitable to improve the formulations used in dietary strategies for subjects with obesity or type 2 diabetes.
33200762	0	37	theme	protein	11:17	arg1	concentrate					19:29	Black bean protein concentrate	0:29	Black bean protein concentrate	0:29	Black bean protein concentrate ameliorates hepatic steatosis by decreasing lipogenesis and increasing fatty acid oxidation in rats fed a high fat-sucrose diet.
33200762	1	38	theme	recent	276:281	arg1	decades					283:289	recent decades	276:289	recent decades	276:289	The black bean is a legume widely consumed in Latin America, however its consumption has decreased significantly in recent decades.
33200762	2	39	from	part	374:377	arg1	due					367:369	due	367:369	due	367:369	There is evidence that its consumption generates beneficial health effects due in part to the type of protein, resistant starches and polyphenols.
33200762	10	40	theme	black	1695:1699	arg1	beans					1701:1705	black beans	1695:1705	black beans	1695:1705	The addition of legumes to the diet of subjects with insulin resistance, including black beans, could improve their metabolic status.
33200762	8	41	theme	normal	1260:1265	arg1	tolerance					1275:1283	normal glucose tolerance	1260:1283	normal glucose tolerance	1260:1283	As a result, rats fed a bean-based diet had lower circulating glucose and insulin concentrations and normal glucose tolerance, which was associated with decreased expression of lipogenic genes in the liver.
33200762	8	41	theme	normal	1260:1265	arg1	concentrations					1241:1254	lower circulating glucose and insulin concentrations	1203:1254	lower circulating glucose and insulin concentrations	1203:1254	As a result, rats fed a bean-based diet had lower circulating glucose and insulin concentrations and normal glucose tolerance, which was associated with decreased expression of lipogenic genes in the liver.
33200762	8	41	theme	normal	1260:1265	arg1	result					1164:1169	a result	1162:1169	a result	1162:1169	As a result, rats fed a bean-based diet had lower circulating glucose and insulin concentrations and normal glucose tolerance, which was associated with decreased expression of lipogenic genes in the liver.
33200762	3	42	theme	food	458:461	arg1	formulation					463:473	food formulation	458:473	food formulation	458:473	Thus, their use in food formulation could impact health status.
33200762	7	43	theme	fuel	1140:1143	arg1	availability					1145:1156	fuel availability	1140:1156	fuel availability	1140:1156	This change was associated with a significant increase in energy expenditure and the capacity to adapt fuel oxidation to fuel availability.
33200762	10	44	theme	metabolic	1728:1736	arg1	status					1738:1743	their metabolic status	1722:1743	their metabolic status	1722:1743	The addition of legumes to the diet of subjects with insulin resistance, including black beans, could improve their metabolic status.
33200762	4	45	theme	energy	709:714	arg1	expenditure					716:726	energy expenditure	709:726	energy expenditure	709:726	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	8	46	theme	insulin	1233:1239	arg1	tolerance					1275:1283	normal glucose tolerance	1260:1283	normal glucose tolerance	1260:1283	As a result, rats fed a bean-based diet had lower circulating glucose and insulin concentrations and normal glucose tolerance, which was associated with decreased expression of lipogenic genes in the liver.
33200762	8	46	theme	insulin	1233:1239	arg1	concentrations					1241:1254	lower circulating glucose and insulin concentrations	1203:1254	lower circulating glucose and insulin concentrations	1203:1254	As a result, rats fed a bean-based diet had lower circulating glucose and insulin concentrations and normal glucose tolerance, which was associated with decreased expression of lipogenic genes in the liver.
33200762	8	46	theme	insulin	1233:1239	arg1	result					1164:1169	a result	1162:1169	a result	1162:1169	As a result, rats fed a bean-based diet had lower circulating glucose and insulin concentrations and normal glucose tolerance, which was associated with decreased expression of lipogenic genes in the liver.
33200762	2	47	theme	due	367:369	arg1	effects					359:365	beneficial health effects	341:365	beneficial health effects due in part to the type of protein, resistant starches and polyphenols	341:436	There is evidence that its consumption generates beneficial health effects due in part to the type of protein, resistant starches and polyphenols.
33200762	6	48	theme	body	966:969	arg1	fat					971:973	body fat	966:973	body fat	966:973	The results showed that consumption of either BPC or WCB reduced weight gain and body fat despite the consumption of a high-fat diet.
33200762	2	49	theme	health	352:357	arg1	effects					359:365	beneficial health effects	341:365	beneficial health effects due in part to the type of protein, resistant starches and polyphenols	341:436	There is evidence that its consumption generates beneficial health effects due in part to the type of protein, resistant starches and polyphenols.
33200762	4	50	theme	work	541:544	arg1	purpose					518:524	the purpose	514:524	the purpose of the present work	514:544	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	4	51	theme	5	785:785	arg1	%					786:786	%	786:786	%	786:786	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	1	52	theme	black	164:168	arg1	bean					170:173	The black bean	160:173	The black bean	160:173	The black bean is a legume widely consumed in Latin America, however its consumption has decreased significantly in recent decades.
33200762	1	52	theme	black	164:168	arg1	legume					180:185	a legume	178:185	a legume widely consumed in Latin America	178:218	The black bean is a legume widely consumed in Latin America, however its consumption has decreased significantly in recent decades.
33200762	4	53	theme	control	749:755	arg1	diet					757:760	a control diet	747:760	a control diet	747:760	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	4	54	theme	protein	603:609	arg1	concentrate					611:621	a bean protein concentrate	596:621	a bean protein concentrate (BPC)	596:627	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	4	54	theme	protein	603:609	arg1	BPC					624:626	BPC	624:626	BPC	624:626	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	9	55	theme	dietary	1547:1553	arg1	strategies					1555:1564	dietary strategies	1547:1564	dietary strategies for subjects with obesity or type 2 diabetes	1547:1609	These results suggest that the type of protein and bioactive compounds particularly phenolic and flavonoid compounds present in BPC are suitable to improve the formulations used in dietary strategies for subjects with obesity or type 2 diabetes.
33200762	2	56	from	due	367:369	arg1	part					374:377	part	374:377	part	374:377	There is evidence that its consumption generates beneficial health effects due in part to the type of protein, resistant starches and polyphenols.
33200762	6	57	theme	diet	1013:1016	arg1	consumption					987:997	the consumption	983:997	the consumption of a high-fat diet	983:1016	The results showed that consumption of either BPC or WCB reduced weight gain and body fat despite the consumption of a high-fat diet.
33200762	10	58	theme	legumes	1628:1634	arg1	addition					1616:1623	The addition	1612:1623	The addition of legumes to the diet of subjects with insulin resistance, including black beans,	1612:1706	The addition of legumes to the diet of subjects with insulin resistance, including black beans, could improve their metabolic status.
33200762	8	59	dep	had	1199:1201	arg1	associated					1296:1305	associated	1296:1305	was associated with decreased expression of lipogenic genes in the liver	1292:1363	As a result, rats fed a bean-based diet had lower circulating glucose and insulin concentrations and normal glucose tolerance, which was associated with decreased expression of lipogenic genes in the liver.
33200762	4	60	theme	consumption	581:591	arg1	effects					566:572	the effects	562:572	the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water	562:816	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	0	61	theme	high	137:140	arg1	diet					154:157	a high fat-sucrose diet	135:157	a high fat-sucrose diet	135:157	Black bean protein concentrate ameliorates hepatic steatosis by decreasing lipogenesis and increasing fatty acid oxidation in rats fed a high fat-sucrose diet.
33200762	8	62	theme	decreased	1312:1320	arg1	expression					1322:1331	decreased expression	1312:1331	decreased expression of lipogenic genes in the liver	1312:1363	As a result, rats fed a bean-based diet had lower circulating glucose and insulin concentrations and normal glucose tolerance, which was associated with decreased expression of lipogenic genes in the liver.
33200762	7	63	theme	significant	1053:1063	arg1	increase					1065:1072	a significant increase	1051:1072	a significant increase in energy expenditure	1051:1094	This change was associated with a significant increase in energy expenditure and the capacity to adapt fuel oxidation to fuel availability.
33200762	4	64	theme	cooked	641:646	arg1	WCB					660:662	WCB	660:662	WCB	660:662	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	4	64	theme	cooked	641:646	arg1	flour					653:657	a whole cooked bean flour	633:657	a whole cooked bean flour (WCB)	633:663	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	8	65	theme	genes	1346:1350	arg1	expression					1322:1331	decreased expression	1312:1331	decreased expression of lipogenic genes in the liver	1312:1363	As a result, rats fed a bean-based diet had lower circulating glucose and insulin concentrations and normal glucose tolerance, which was associated with decreased expression of lipogenic genes in the liver.
33200762	4	66	theme	flour	653:657	arg1	consumption					581:591	the consumption	577:591	the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB)	577:663	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	4	67	from	sucrose	788:794	arg1	water					812:816	the drinking water	799:816	the drinking water	799:816	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	4	68	from	expenditure	716:726	arg1	rats					738:741	Wistar rats	731:741	Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water	731:816	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	4	69	with	diets	774:778	arg1	sucrose					788:794	5% sucrose	785:794	5% sucrose in the drinking water	785:816	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	4	70	from	effects	566:572	arg1	expenditure					716:726	energy expenditure	709:726	energy expenditure	709:726	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	4	70	from	effects	566:572	arg1	composition					673:683	body composition	668:683	body composition	668:683	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	4	70	from	effects	566:572	arg1	metabolism					694:703	glucose metabolism	686:703	glucose metabolism	686:703	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	0	71	theme	acid	108:111	arg1	oxidation					113:121	fatty acid oxidation	102:121	fatty acid oxidation in rats fed a high fat-sucrose diet	102:157	Black bean protein concentrate ameliorates hepatic steatosis by decreasing lipogenesis and increasing fatty acid oxidation in rats fed a high fat-sucrose diet.
33200762	8	72	contain	had	1199:1201	arg2	tolerance					1275:1283	normal glucose tolerance	1260:1283	normal glucose tolerance	1260:1283	As a result, rats fed a bean-based diet had lower circulating glucose and insulin concentrations and normal glucose tolerance, which was associated with decreased expression of lipogenic genes in the liver.
33200762	8	72	contain	had	1199:1201	arg1	rats					1172:1175	rats	1172:1175	rats fed a bean-based diet	1172:1197	As a result, rats fed a bean-based diet had lower circulating glucose and insulin concentrations and normal glucose tolerance, which was associated with decreased expression of lipogenic genes in the liver.
33200762	8	72	contain	had	1199:1201	arg2	result					1164:1169	a result	1162:1169	a result	1162:1169	As a result, rats fed a bean-based diet had lower circulating glucose and insulin concentrations and normal glucose tolerance, which was associated with decreased expression of lipogenic genes in the liver.
33200762	8	72	contain	had	1199:1201	arg2	concentrations					1241:1254	lower circulating glucose and insulin concentrations	1203:1254	lower circulating glucose and insulin concentrations	1203:1254	As a result, rats fed a bean-based diet had lower circulating glucose and insulin concentrations and normal glucose tolerance, which was associated with decreased expression of lipogenic genes in the liver.
33200762	4	73	from	composition	673:683	arg1	rats					738:741	Wistar rats	731:741	Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water	731:816	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	5	74	theme	experimental	852:863	arg1	diets					865:869	the experimental diets	848:869	the experimental diets	848:869	With this aim, rats were fed the experimental diets for 10 weeks.
33200762	6	75	theme	weight	950:955	arg1	gain					957:960	weight gain	950:960	weight gain	950:960	The results showed that consumption of either BPC or WCB reduced weight gain and body fat despite the consumption of a high-fat diet.
33200762	4	76	theme	glucose	686:692	arg1	metabolism					694:703	glucose metabolism	686:703	glucose metabolism	686:703	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	0	77	theme	bean	6:9	arg1	concentrate					19:29	Black bean protein concentrate	0:29	Black bean protein concentrate	0:29	Black bean protein concentrate ameliorates hepatic steatosis by decreasing lipogenesis and increasing fatty acid oxidation in rats fed a high fat-sucrose diet.
33200762	6	78	theme	WCB	938:940	arg1	consumption					909:919	consumption	909:919	consumption of either BPC or WCB	909:940	The results showed that consumption of either BPC or WCB reduced weight gain and body fat despite the consumption of a high-fat diet.
33200762	6	79	theme	BPC	931:933	arg1	consumption					909:919	consumption	909:919	consumption of either BPC or WCB	909:940	The results showed that consumption of either BPC or WCB reduced weight gain and body fat despite the consumption of a high-fat diet.
33200762	2	80	theme	resistant	403:411	arg1	starches					413:420	resistant starches	403:420	resistant starches	403:420	There is evidence that its consumption generates beneficial health effects due in part to the type of protein, resistant starches and polyphenols.
33200762	4	81	theme	present	533:539	arg1	work					541:544	the present work	529:544	the present work	529:544	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	8	82	theme	glucose	1221:1227	arg1	tolerance					1275:1283	normal glucose tolerance	1260:1283	normal glucose tolerance	1260:1283	As a result, rats fed a bean-based diet had lower circulating glucose and insulin concentrations and normal glucose tolerance, which was associated with decreased expression of lipogenic genes in the liver.
33200762	8	82	theme	glucose	1221:1227	arg1	concentrations					1241:1254	lower circulating glucose and insulin concentrations	1203:1254	lower circulating glucose and insulin concentrations	1203:1254	As a result, rats fed a bean-based diet had lower circulating glucose and insulin concentrations and normal glucose tolerance, which was associated with decreased expression of lipogenic genes in the liver.
33200762	8	82	theme	glucose	1221:1227	arg1	result					1164:1169	a result	1162:1169	a result	1162:1169	As a result, rats fed a bean-based diet had lower circulating glucose and insulin concentrations and normal glucose tolerance, which was associated with decreased expression of lipogenic genes in the liver.
33200762	2	83	theme	protein	394:400	arg1	type					386:389	the type	382:389	the type of protein, resistant starches and polyphenols	382:436	There is evidence that its consumption generates beneficial health effects due in part to the type of protein, resistant starches and polyphenols.
33200762	0	84	theme	hepatic	43:49	arg1	steatosis					51:59	hepatic steatosis	43:59	hepatic steatosis	43:59	Black bean protein concentrate ameliorates hepatic steatosis by decreasing lipogenesis and increasing fatty acid oxidation in rats fed a high fat-sucrose diet.
33200762	9	85	theme	phenolic	1450:1457	arg1	compounds					1427:1435	bioactive compounds	1417:1435	bioactive compounds particularly phenolic	1417:1457	These results suggest that the type of protein and bioactive compounds particularly phenolic and flavonoid compounds present in BPC are suitable to improve the formulations used in dietary strategies for subjects with obesity or type 2 diabetes.
33200762	4	86	theme	Wistar	731:736	arg1	rats					738:741	Wistar rats	731:741	Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water	731:816	Therefore, the purpose of the present work was to evaluate the effects of the consumption of a bean protein concentrate (BPC) and a whole cooked bean flour (WCB) on body composition, glucose metabolism and energy expenditure in Wistar rats fed a control diet or high-fat diets with 5% sucrose in the drinking water.
33200762	9	87	theme	flavonoid	1463:1471	arg1	compounds					1473:1481	flavonoid compounds	1463:1481	flavonoid compounds	1463:1481	These results suggest that the type of protein and bioactive compounds particularly phenolic and flavonoid compounds present in BPC are suitable to improve the formulations used in dietary strategies for subjects with obesity or type 2 diabetes.
33200762	9	88	theme	present	1483:1489	arg1	compounds					1427:1435	bioactive compounds	1417:1435	bioactive compounds particularly phenolic	1417:1457	These results suggest that the type of protein and bioactive compounds particularly phenolic and flavonoid compounds present in BPC are suitable to improve the formulations used in dietary strategies for subjects with obesity or type 2 diabetes.
33200762	7	89	theme	fuel	1122:1125	arg1	oxidation					1127:1135	fuel oxidation	1122:1135	fuel oxidation	1122:1135	This change was associated with a significant increase in energy expenditure and the capacity to adapt fuel oxidation to fuel availability.
33200762	8	90	theme	glucose	1267:1273	arg1	tolerance					1275:1283	normal glucose tolerance	1260:1283	normal glucose tolerance	1260:1283	As a result, rats fed a bean-based diet had lower circulating glucose and insulin concentrations and normal glucose tolerance, which was associated with decreased expression of lipogenic genes in the liver.
33200762	8	90	theme	glucose	1267:1273	arg1	concentrations					1241:1254	lower circulating glucose and insulin concentrations	1203:1254	lower circulating glucose and insulin concentrations	1203:1254	As a result, rats fed a bean-based diet had lower circulating glucose and insulin concentrations and normal glucose tolerance, which was associated with decreased expression of lipogenic genes in the liver.
33200762	8	90	theme	glucose	1267:1273	arg1	result					1164:1169	a result	1162:1169	a result	1162:1169	As a result, rats fed a bean-based diet had lower circulating glucose and insulin concentrations and normal glucose tolerance, which was associated with decreased expression of lipogenic genes in the liver.
33807577	0	0	theme	Adenoma	96:102	arg1	Development					58:68	the Development	54:68	the Development of Spontaneous Intestinal Adenoma	54:102	Consumption of Select Dietary Emulsifiers Exacerbates the Development of Spontaneous Intestinal Adenoma.
33807577	6	1	theme	emulsifier	1189:1198	arg1	consumption					1200:1210	emulsifier consumption	1189:1210	emulsifier consumption	1189:1210	Overall, our findings further support the hypothesis that emulsifier consumption may be a new modifiable risk factor for colorectal cancer (CRC) and that alterations in host-microbiota interactions can favor gastrointestinal carcinogenesis in individuals with a genetical predisposition to such disorders.
33807577	6	1	theme	emulsifier	1189:1198	arg1	factor					1241:1246	a new modifiable risk factor	1219:1246	a new modifiable risk factor for colorectal cancer (CRC)	1219:1274	Overall, our findings further support the hypothesis that emulsifier consumption may be a new modifiable risk factor for colorectal cancer (CRC) and that alterations in host-microbiota interactions can favor gastrointestinal carcinogenesis in individuals with a genetical predisposition to such disorders.
33807577	4	2	theme	dietary	651:657	arg1	consumption					671:681	dietary emulsifiers consumption	651:681	dietary emulsifiers consumption	651:681	Here, we investigate the impact of dietary emulsifiers consumption on cancer initiation and progression in a genetical model of intestinal adenomas.
33807577	0	3	theme	Intestinal	85:94	arg1	Adenoma					96:102	Spontaneous Intestinal Adenoma	73:102	Spontaneous Intestinal Adenoma	73:102	Consumption of Select Dietary Emulsifiers Exacerbates the Development of Spontaneous Intestinal Adenoma.
33807577	6	4	theme	genetical	1393:1401	arg1	predisposition					1403:1416	a genetical predisposition	1391:1416	a genetical predisposition to such disorders	1391:1434	Overall, our findings further support the hypothesis that emulsifier consumption may be a new modifiable risk factor for colorectal cancer (CRC) and that alterations in host-microbiota interactions can favor gastrointestinal carcinogenesis in individuals with a genetical predisposition to such disorders.
33807577	3	5	theme	exacerbated	577:587	arg1	development					603:613	polysorbate-80 exacerbated colonic tumor development	562:613	polysorbate-80 exacerbated colonic tumor development	562:613	Using a model of colitis-associated cancer, we previously reported that consumption of the dietary emulsifiers carboxymethylcellulose or polysorbate-80 exacerbated colonic tumor development.
33807577	6	6	with	individuals	1374:1384	arg1	predisposition					1403:1416	a genetical predisposition	1391:1416	a genetical predisposition to such disorders	1391:1434	Overall, our findings further support the hypothesis that emulsifier consumption may be a new modifiable risk factor for colorectal cancer (CRC) and that alterations in host-microbiota interactions can favor gastrointestinal carcinogenesis in individuals with a genetical predisposition to such disorders.
33807577	5	7	theme	dietary	798:804	arg1	consumption					818:828	dietary emulsifiers consumption	798:828	dietary emulsifiers consumption	798:828	In APCmin mice, we observed that dietary emulsifiers consumption enhanced small-intestine tumor development in a way that appeared to be independent of chronic intestinal inflammation but rather associated with emulsifiers' impact on the proliferative status of the intestinal epithelium as well as on intestinal microbiota composition in both male and female mice.
33807577	3	8	theme	colonic	589:595	arg1	development					603:613	polysorbate-80 exacerbated colonic tumor development	562:613	polysorbate-80 exacerbated colonic tumor development	562:613	Using a model of colitis-associated cancer, we previously reported that consumption of the dietary emulsifiers carboxymethylcellulose or polysorbate-80 exacerbated colonic tumor development.
33807577	6	9	theme	risk	1236:1239	arg1	consumption					1200:1210	emulsifier consumption	1189:1210	emulsifier consumption	1189:1210	Overall, our findings further support the hypothesis that emulsifier consumption may be a new modifiable risk factor for colorectal cancer (CRC) and that alterations in host-microbiota interactions can favor gastrointestinal carcinogenesis in individuals with a genetical predisposition to such disorders.
33807577	6	9	theme	risk	1236:1239	arg1	factor					1241:1246	a new modifiable risk factor	1219:1246	a new modifiable risk factor for colorectal cancer (CRC)	1219:1274	Overall, our findings further support the hypothesis that emulsifier consumption may be a new modifiable risk factor for colorectal cancer (CRC) and that alterations in host-microbiota interactions can favor gastrointestinal carcinogenesis in individuals with a genetical predisposition to such disorders.
33807577	4	10	theme	consumption	671:681	arg1	impact					641:646	the impact	637:646	the impact of dietary emulsifiers consumption on cancer initiation and progression in a genetical model of intestinal adenomas	637:762	Here, we investigate the impact of dietary emulsifiers consumption on cancer initiation and progression in a genetical model of intestinal adenomas.
33807577	5	11	theme	emulsifiers	806:816	arg1	consumption					818:828	dietary emulsifiers consumption	798:828	dietary emulsifiers consumption	798:828	In APCmin mice, we observed that dietary emulsifiers consumption enhanced small-intestine tumor development in a way that appeared to be independent of chronic intestinal inflammation but rather associated with emulsifiers' impact on the proliferative status of the intestinal epithelium as well as on intestinal microbiota composition in both male and female mice.
33807577	2	12	theme	Previous	187:194	arg1	findings					196:203	Previous findings	187:203	Previous findings	187:203	Previous findings have shown that intestinal low-grade inflammation can be promoted by the consumption of select dietary emulsifiers, ubiquitous component of processed foods which alter the composition and function of the gut microbiota.
33807577	2	13	theme	ubiquitous	321:330	arg1	emulsifiers					308:318	select dietary emulsifiers	293:318	select dietary emulsifiers	293:318	Previous findings have shown that intestinal low-grade inflammation can be promoted by the consumption of select dietary emulsifiers, ubiquitous component of processed foods which alter the composition and function of the gut microbiota.
33807577	2	13	theme	ubiquitous	321:330	arg1	component					332:340	ubiquitous component	321:340	ubiquitous component of processed foods which alter the composition and function of the gut microbiota	321:422	Previous findings have shown that intestinal low-grade inflammation can be promoted by the consumption of select dietary emulsifiers, ubiquitous component of processed foods which alter the composition and function of the gut microbiota.
33807577	4	14	theme	emulsifiers	659:669	arg1	consumption					671:681	dietary emulsifiers consumption	651:681	dietary emulsifiers consumption	651:681	Here, we investigate the impact of dietary emulsifiers consumption on cancer initiation and progression in a genetical model of intestinal adenomas.
33807577	5	15	theme	proliferative	1003:1015	arg1	status					1017:1022	the proliferative status	999:1022	the proliferative status of the intestinal epithelium	999:1051	In APCmin mice, we observed that dietary emulsifiers consumption enhanced small-intestine tumor development in a way that appeared to be independent of chronic intestinal inflammation but rather associated with emulsifiers' impact on the proliferative status of the intestinal epithelium as well as on intestinal microbiota composition in both male and female mice.
33807577	5	16	theme	intestinal	925:934	arg1	inflammation					936:947	chronic intestinal inflammation	917:947	chronic intestinal inflammation but rather associated with emulsifiers' impact on the proliferative status of the intestinal epithelium as well as on intestinal microbiota composition in both male and female mice	917:1128	In APCmin mice, we observed that dietary emulsifiers consumption enhanced small-intestine tumor development in a way that appeared to be independent of chronic intestinal inflammation but rather associated with emulsifiers' impact on the proliferative status of the intestinal epithelium as well as on intestinal microbiota composition in both male and female mice.
33807577	5	17	theme	intestinal	1067:1076	arg1	composition					1089:1099	intestinal microbiota composition	1067:1099	intestinal microbiota composition in both male and female mice	1067:1128	In APCmin mice, we observed that dietary emulsifiers consumption enhanced small-intestine tumor development in a way that appeared to be independent of chronic intestinal inflammation but rather associated with emulsifiers' impact on the proliferative status of the intestinal epithelium as well as on intestinal microbiota composition in both male and female mice.
33807577	6	18	from	alterations	1285:1295	arg1	interactions					1316:1327	host-microbiota interactions	1300:1327	host-microbiota interactions	1300:1327	Overall, our findings further support the hypothesis that emulsifier consumption may be a new modifiable risk factor for colorectal cancer (CRC) and that alterations in host-microbiota interactions can favor gastrointestinal carcinogenesis in individuals with a genetical predisposition to such disorders.
33807577	6	19	from	carcinogenesis	1356:1369	arg1	individuals					1374:1384	individuals	1374:1384	individuals with a genetical predisposition to such disorders	1374:1434	Overall, our findings further support the hypothesis that emulsifier consumption may be a new modifiable risk factor for colorectal cancer (CRC) and that alterations in host-microbiota interactions can favor gastrointestinal carcinogenesis in individuals with a genetical predisposition to such disorders.
33807577	2	20	theme	emulsifiers	308:318	arg1	consumption					278:288	the consumption	274:288	the consumption of select dietary emulsifiers, ubiquitous component of processed foods which alter the composition and function of the gut microbiota	274:422	Previous findings have shown that intestinal low-grade inflammation can be promoted by the consumption of select dietary emulsifiers, ubiquitous component of processed foods which alter the composition and function of the gut microbiota.
33807577	5	21	from	composition	1089:1099	arg1	mice					1125:1128	both male and female mice	1104:1128	both male and female mice	1104:1128	In APCmin mice, we observed that dietary emulsifiers consumption enhanced small-intestine tumor development in a way that appeared to be independent of chronic intestinal inflammation but rather associated with emulsifiers' impact on the proliferative status of the intestinal epithelium as well as on intestinal microbiota composition in both male and female mice.
33807577	4	22	from	impact	641:646	arg1	progression					708:718	progression	708:718	progression	708:718	Here, we investigate the impact of dietary emulsifiers consumption on cancer initiation and progression in a genetical model of intestinal adenomas.
33807577	4	22	from	impact	641:646	arg1	initiation					693:702	cancer initiation	686:702	cancer initiation	686:702	Here, we investigate the impact of dietary emulsifiers consumption on cancer initiation and progression in a genetical model of intestinal adenomas.
33807577	6	23	theme	colorectal	1252:1261	arg1	CRC					1271:1273	CRC	1271:1273	CRC	1271:1273	Overall, our findings further support the hypothesis that emulsifier consumption may be a new modifiable risk factor for colorectal cancer (CRC) and that alterations in host-microbiota interactions can favor gastrointestinal carcinogenesis in individuals with a genetical predisposition to such disorders.
33807577	6	23	theme	colorectal	1252:1261	arg1	cancer					1263:1268	colorectal cancer	1252:1268	colorectal cancer (CRC)	1252:1274	Overall, our findings further support the hypothesis that emulsifier consumption may be a new modifiable risk factor for colorectal cancer (CRC) and that alterations in host-microbiota interactions can favor gastrointestinal carcinogenesis in individuals with a genetical predisposition to such disorders.
33807577	5	24	theme	small-intestine	839:853	arg1	development					861:871	small-intestine tumor development	839:871	small-intestine tumor development	839:871	In APCmin mice, we observed that dietary emulsifiers consumption enhanced small-intestine tumor development in a way that appeared to be independent of chronic intestinal inflammation but rather associated with emulsifiers' impact on the proliferative status of the intestinal epithelium as well as on intestinal microbiota composition in both male and female mice.
33807577	5	25	theme	intestinal	1031:1040	arg1	epithelium					1042:1051	the intestinal epithelium	1027:1051	the intestinal epithelium	1027:1051	In APCmin mice, we observed that dietary emulsifiers consumption enhanced small-intestine tumor development in a way that appeared to be independent of chronic intestinal inflammation but rather associated with emulsifiers' impact on the proliferative status of the intestinal epithelium as well as on intestinal microbiota composition in both male and female mice.
33807577	4	26	theme	adenomas	755:762	arg1	model					735:739	a genetical model	723:739	a genetical model of intestinal adenomas	723:762	Here, we investigate the impact of dietary emulsifiers consumption on cancer initiation and progression in a genetical model of intestinal adenomas.
33807577	6	27	theme	gastrointestinal	1339:1354	arg1	carcinogenesis					1356:1369	gastrointestinal carcinogenesis	1339:1369	gastrointestinal carcinogenesis in individuals with a genetical predisposition to such disorders	1339:1434	Overall, our findings further support the hypothesis that emulsifier consumption may be a new modifiable risk factor for colorectal cancer (CRC) and that alterations in host-microbiota interactions can favor gastrointestinal carcinogenesis in individuals with a genetical predisposition to such disorders.
33807577	2	28	theme	foods	355:359	arg1	emulsifiers					308:318	select dietary emulsifiers	293:318	select dietary emulsifiers	293:318	Previous findings have shown that intestinal low-grade inflammation can be promoted by the consumption of select dietary emulsifiers, ubiquitous component of processed foods which alter the composition and function of the gut microbiota.
33807577	2	28	theme	foods	355:359	arg1	component					332:340	ubiquitous component	321:340	ubiquitous component of processed foods which alter the composition and function of the gut microbiota	321:422	Previous findings have shown that intestinal low-grade inflammation can be promoted by the consumption of select dietary emulsifiers, ubiquitous component of processed foods which alter the composition and function of the gut microbiota.
33807577	2	29	theme	microbiota	413:422	arg1	composition					377:387	composition	377:387	composition	377:387	Previous findings have shown that intestinal low-grade inflammation can be promoted by the consumption of select dietary emulsifiers, ubiquitous component of processed foods which alter the composition and function of the gut microbiota.
33807577	2	29	theme	microbiota	413:422	arg1	function					393:400	function	393:400	function	393:400	Previous findings have shown that intestinal low-grade inflammation can be promoted by the consumption of select dietary emulsifiers, ubiquitous component of processed foods which alter the composition and function of the gut microbiota.
33807577	0	30	theme	Dietary	22:28	arg1	Emulsifiers					30:40	Select Dietary Emulsifiers	15:40	Select Dietary Emulsifiers	15:40	Consumption of Select Dietary Emulsifiers Exacerbates the Development of Spontaneous Intestinal Adenoma.
33807577	5	31	from	impact	989:994	arg1	status					1017:1022	the proliferative status	999:1022	the proliferative status of the intestinal epithelium	999:1051	In APCmin mice, we observed that dietary emulsifiers consumption enhanced small-intestine tumor development in a way that appeared to be independent of chronic intestinal inflammation but rather associated with emulsifiers' impact on the proliferative status of the intestinal epithelium as well as on intestinal microbiota composition in both male and female mice.
33807577	2	32	theme	processed	345:353	arg1	foods					355:359	processed foods	345:359	processed foods which alter the composition and function of the gut microbiota	345:422	Previous findings have shown that intestinal low-grade inflammation can be promoted by the consumption of select dietary emulsifiers, ubiquitous component of processed foods which alter the composition and function of the gut microbiota.
33807577	5	33	theme	chronic	917:923	arg1	inflammation					936:947	chronic intestinal inflammation	917:947	chronic intestinal inflammation but rather associated with emulsifiers' impact on the proliferative status of the intestinal epithelium as well as on intestinal microbiota composition in both male and female mice	917:1128	In APCmin mice, we observed that dietary emulsifiers consumption enhanced small-intestine tumor development in a way that appeared to be independent of chronic intestinal inflammation but rather associated with emulsifiers' impact on the proliferative status of the intestinal epithelium as well as on intestinal microbiota composition in both male and female mice.
33807577	0	34	theme	Select	15:20	arg1	Emulsifiers					30:40	Select Dietary Emulsifiers	15:40	Select Dietary Emulsifiers	15:40	Consumption of Select Dietary Emulsifiers Exacerbates the Development of Spontaneous Intestinal Adenoma.
33807577	5	35	theme	epithelium	1042:1051	arg1	status					1017:1022	the proliferative status	999:1022	the proliferative status of the intestinal epithelium	999:1051	In APCmin mice, we observed that dietary emulsifiers consumption enhanced small-intestine tumor development in a way that appeared to be independent of chronic intestinal inflammation but rather associated with emulsifiers' impact on the proliferative status of the intestinal epithelium as well as on intestinal microbiota composition in both male and female mice.
33807577	2	36	dep	composition	377:387	arg1	the					373:375	the	373:375	the	373:375	Previous findings have shown that intestinal low-grade inflammation can be promoted by the consumption of select dietary emulsifiers, ubiquitous component of processed foods which alter the composition and function of the gut microbiota.
33807577	1	37	theme	gastrointestinal	161:176	arg1	cancers					178:184	gastrointestinal cancers	161:184	gastrointestinal cancers	161:184	Inflammation is a well-characterized critical driver of gastrointestinal cancers.
33807577	3	38	theme	colitis-associated	442:459	arg1	cancer					461:466	colitis-associated cancer	442:466	colitis-associated cancer	442:466	Using a model of colitis-associated cancer, we previously reported that consumption of the dietary emulsifiers carboxymethylcellulose or polysorbate-80 exacerbated colonic tumor development.
33807577	6	39	theme	new	1221:1223	arg1	consumption					1200:1210	emulsifier consumption	1189:1210	emulsifier consumption	1189:1210	Overall, our findings further support the hypothesis that emulsifier consumption may be a new modifiable risk factor for colorectal cancer (CRC) and that alterations in host-microbiota interactions can favor gastrointestinal carcinogenesis in individuals with a genetical predisposition to such disorders.
33807577	6	39	theme	new	1221:1223	arg1	factor					1241:1246	a new modifiable risk factor	1219:1246	a new modifiable risk factor for colorectal cancer (CRC)	1219:1274	Overall, our findings further support the hypothesis that emulsifier consumption may be a new modifiable risk factor for colorectal cancer (CRC) and that alterations in host-microbiota interactions can favor gastrointestinal carcinogenesis in individuals with a genetical predisposition to such disorders.
33807577	5	40	theme	inflammation	936:947	arg1	independent					902:912	independent	902:912	independent	902:912	In APCmin mice, we observed that dietary emulsifiers consumption enhanced small-intestine tumor development in a way that appeared to be independent of chronic intestinal inflammation but rather associated with emulsifiers' impact on the proliferative status of the intestinal epithelium as well as on intestinal microbiota composition in both male and female mice.
33807577	5	40	theme	inflammation	936:947	arg1	way					878:880	a way	876:880	a way that appeared to be independent of chronic intestinal inflammation but rather associated with emulsifiers' impact on the proliferative status of the intestinal epithelium as well as on intestinal microbiota composition in both male and female mice	876:1128	In APCmin mice, we observed that dietary emulsifiers consumption enhanced small-intestine tumor development in a way that appeared to be independent of chronic intestinal inflammation but rather associated with emulsifiers' impact on the proliferative status of the intestinal epithelium as well as on intestinal microbiota composition in both male and female mice.
33807577	4	41	theme	intestinal	744:753	arg1	adenomas					755:762	intestinal adenomas	744:762	intestinal adenomas	744:762	Here, we investigate the impact of dietary emulsifiers consumption on cancer initiation and progression in a genetical model of intestinal adenomas.
33807577	4	42	theme	genetical	725:733	arg1	model					735:739	a genetical model	723:739	a genetical model of intestinal adenomas	723:762	Here, we investigate the impact of dietary emulsifiers consumption on cancer initiation and progression in a genetical model of intestinal adenomas.
33807577	0	43	theme	Emulsifiers	30:40	arg1	Consumption					0:10	Consumption	0:10	Consumption of Select Dietary Emulsifiers	0:40	Consumption of Select Dietary Emulsifiers Exacerbates the Development of Spontaneous Intestinal Adenoma.
33807577	3	44	theme	cancer	461:466	arg1	model					433:437	a model	431:437	a model of colitis-associated cancer	431:466	Using a model of colitis-associated cancer, we previously reported that consumption of the dietary emulsifiers carboxymethylcellulose or polysorbate-80 exacerbated colonic tumor development.
33807577	5	45	theme	female	1118:1123	arg1	mice					1125:1128	both male and female mice	1104:1128	both male and female mice	1104:1128	In APCmin mice, we observed that dietary emulsifiers consumption enhanced small-intestine tumor development in a way that appeared to be independent of chronic intestinal inflammation but rather associated with emulsifiers' impact on the proliferative status of the intestinal epithelium as well as on intestinal microbiota composition in both male and female mice.
33807577	5	46	theme	tumor	855:859	arg1	development					861:871	small-intestine tumor development	839:871	small-intestine tumor development	839:871	In APCmin mice, we observed that dietary emulsifiers consumption enhanced small-intestine tumor development in a way that appeared to be independent of chronic intestinal inflammation but rather associated with emulsifiers' impact on the proliferative status of the intestinal epithelium as well as on intestinal microbiota composition in both male and female mice.
33807577	6	47	theme	such	1421:1424	arg1	disorders					1426:1434	such disorders	1421:1434	such disorders	1421:1434	Overall, our findings further support the hypothesis that emulsifier consumption may be a new modifiable risk factor for colorectal cancer (CRC) and that alterations in host-microbiota interactions can favor gastrointestinal carcinogenesis in individuals with a genetical predisposition to such disorders.
33807577	1	48	theme	well-characterized	123:140	arg1	driver					151:156	a well-characterized critical driver	121:156	a well-characterized critical driver of gastrointestinal cancers	121:184	Inflammation is a well-characterized critical driver of gastrointestinal cancers.
33807577	1	48	theme	well-characterized	123:140	arg1	Inflammation					105:116	Inflammation	105:116	Inflammation	105:116	Inflammation is a well-characterized critical driver of gastrointestinal cancers.
33807577	6	49	theme	host-microbiota	1300:1314	arg1	interactions					1316:1327	host-microbiota interactions	1300:1327	host-microbiota interactions	1300:1327	Overall, our findings further support the hypothesis that emulsifier consumption may be a new modifiable risk factor for colorectal cancer (CRC) and that alterations in host-microbiota interactions can favor gastrointestinal carcinogenesis in individuals with a genetical predisposition to such disorders.
33807577	1	50	theme	critical	142:149	arg1	driver					151:156	a well-characterized critical driver	121:156	a well-characterized critical driver of gastrointestinal cancers	121:184	Inflammation is a well-characterized critical driver of gastrointestinal cancers.
33807577	1	50	theme	critical	142:149	arg1	Inflammation					105:116	Inflammation	105:116	Inflammation	105:116	Inflammation is a well-characterized critical driver of gastrointestinal cancers.
33807577	5	51	theme	APCmin	768:773	arg1	mice					775:778	APCmin mice	768:778	APCmin mice	768:778	In APCmin mice, we observed that dietary emulsifiers consumption enhanced small-intestine tumor development in a way that appeared to be independent of chronic intestinal inflammation but rather associated with emulsifiers' impact on the proliferative status of the intestinal epithelium as well as on intestinal microbiota composition in both male and female mice.
33807577	5	52	dep	inflammation	936:947	arg1	associated					960:969	associated	960:969	but rather associated with emulsifiers' impact on the proliferative status of the intestinal epithelium as well as on intestinal microbiota composition in both male and female mice	949:1128	In APCmin mice, we observed that dietary emulsifiers consumption enhanced small-intestine tumor development in a way that appeared to be independent of chronic intestinal inflammation but rather associated with emulsifiers' impact on the proliferative status of the intestinal epithelium as well as on intestinal microbiota composition in both male and female mice.
33807577	5	52	dep	inflammation	936:947	arg1	composition					1089:1099	intestinal microbiota composition	1067:1099	intestinal microbiota composition in both male and female mice	1067:1128	In APCmin mice, we observed that dietary emulsifiers consumption enhanced small-intestine tumor development in a way that appeared to be independent of chronic intestinal inflammation but rather associated with emulsifiers' impact on the proliferative status of the intestinal epithelium as well as on intestinal microbiota composition in both male and female mice.
33807577	2	53	theme	dietary	300:306	arg1	emulsifiers					308:318	select dietary emulsifiers	293:318	select dietary emulsifiers	293:318	Previous findings have shown that intestinal low-grade inflammation can be promoted by the consumption of select dietary emulsifiers, ubiquitous component of processed foods which alter the composition and function of the gut microbiota.
33807577	2	53	theme	dietary	300:306	arg1	component					332:340	ubiquitous component	321:340	ubiquitous component of processed foods which alter the composition and function of the gut microbiota	321:422	Previous findings have shown that intestinal low-grade inflammation can be promoted by the consumption of select dietary emulsifiers, ubiquitous component of processed foods which alter the composition and function of the gut microbiota.
33807577	3	54	theme	dietary	516:522	arg1	consumption					497:507	consumption	497:507	consumption of the dietary	497:522	Using a model of colitis-associated cancer, we previously reported that consumption of the dietary emulsifiers carboxymethylcellulose or polysorbate-80 exacerbated colonic tumor development.
33807577	4	55	theme	cancer	686:691	arg1	initiation					693:702	cancer initiation	686:702	cancer initiation	686:702	Here, we investigate the impact of dietary emulsifiers consumption on cancer initiation and progression in a genetical model of intestinal adenomas.
33807577	5	56	theme	microbiota	1078:1087	arg1	composition					1089:1099	intestinal microbiota composition	1067:1099	intestinal microbiota composition in both male and female mice	1067:1128	In APCmin mice, we observed that dietary emulsifiers consumption enhanced small-intestine tumor development in a way that appeared to be independent of chronic intestinal inflammation but rather associated with emulsifiers' impact on the proliferative status of the intestinal epithelium as well as on intestinal microbiota composition in both male and female mice.
33807577	2	57	theme	low-grade	232:240	arg1	inflammation					242:253	intestinal low-grade inflammation	221:253	intestinal low-grade inflammation	221:253	Previous findings have shown that intestinal low-grade inflammation can be promoted by the consumption of select dietary emulsifiers, ubiquitous component of processed foods which alter the composition and function of the gut microbiota.
33807577	2	58	theme	select	293:298	arg1	emulsifiers					308:318	select dietary emulsifiers	293:318	select dietary emulsifiers	293:318	Previous findings have shown that intestinal low-grade inflammation can be promoted by the consumption of select dietary emulsifiers, ubiquitous component of processed foods which alter the composition and function of the gut microbiota.
33807577	2	58	theme	select	293:298	arg1	component					332:340	ubiquitous component	321:340	ubiquitous component of processed foods which alter the composition and function of the gut microbiota	321:422	Previous findings have shown that intestinal low-grade inflammation can be promoted by the consumption of select dietary emulsifiers, ubiquitous component of processed foods which alter the composition and function of the gut microbiota.
33807577	3	59	theme	tumor	597:601	arg1	development					603:613	polysorbate-80 exacerbated colonic tumor development	562:613	polysorbate-80 exacerbated colonic tumor development	562:613	Using a model of colitis-associated cancer, we previously reported that consumption of the dietary emulsifiers carboxymethylcellulose or polysorbate-80 exacerbated colonic tumor development.
33807577	6	60	theme	modifiable	1225:1234	arg1	consumption					1200:1210	emulsifier consumption	1189:1210	emulsifier consumption	1189:1210	Overall, our findings further support the hypothesis that emulsifier consumption may be a new modifiable risk factor for colorectal cancer (CRC) and that alterations in host-microbiota interactions can favor gastrointestinal carcinogenesis in individuals with a genetical predisposition to such disorders.
33807577	6	60	theme	modifiable	1225:1234	arg1	factor					1241:1246	a new modifiable risk factor	1219:1246	a new modifiable risk factor for colorectal cancer (CRC)	1219:1274	Overall, our findings further support the hypothesis that emulsifier consumption may be a new modifiable risk factor for colorectal cancer (CRC) and that alterations in host-microbiota interactions can favor gastrointestinal carcinogenesis in individuals with a genetical predisposition to such disorders.
33807577	0	61	theme	Spontaneous	73:83	arg1	Adenoma					96:102	Spontaneous Intestinal Adenoma	73:102	Spontaneous Intestinal Adenoma	73:102	Consumption of Select Dietary Emulsifiers Exacerbates the Development of Spontaneous Intestinal Adenoma.
33807577	2	62	theme	intestinal	221:230	arg1	inflammation					242:253	intestinal low-grade inflammation	221:253	intestinal low-grade inflammation	221:253	Previous findings have shown that intestinal low-grade inflammation can be promoted by the consumption of select dietary emulsifiers, ubiquitous component of processed foods which alter the composition and function of the gut microbiota.
33807577	2	63	theme	gut	409:411	arg1	microbiota					413:422	the gut microbiota	405:422	the gut microbiota	405:422	Previous findings have shown that intestinal low-grade inflammation can be promoted by the consumption of select dietary emulsifiers, ubiquitous component of processed foods which alter the composition and function of the gut microbiota.
33807577	4	64	from	initiation	693:702	arg1	model					735:739	a genetical model	723:739	a genetical model of intestinal adenomas	723:762	Here, we investigate the impact of dietary emulsifiers consumption on cancer initiation and progression in a genetical model of intestinal adenomas.
33807577	4	65	from	progression	708:718	arg1	model					735:739	a genetical model	723:739	a genetical model of intestinal adenomas	723:762	Here, we investigate the impact of dietary emulsifiers consumption on cancer initiation and progression in a genetical model of intestinal adenomas.
33807577	5	66	theme	male	1109:1112	arg1	mice					1125:1128	both male and female mice	1104:1128	both male and female mice	1104:1128	In APCmin mice, we observed that dietary emulsifiers consumption enhanced small-intestine tumor development in a way that appeared to be independent of chronic intestinal inflammation but rather associated with emulsifiers' impact on the proliferative status of the intestinal epithelium as well as on intestinal microbiota composition in both male and female mice.
33807577	1	67	theme	cancers	178:184	arg1	driver					151:156	a well-characterized critical driver	121:156	a well-characterized critical driver of gastrointestinal cancers	121:184	Inflammation is a well-characterized critical driver of gastrointestinal cancers.
33807577	1	67	theme	cancers	178:184	arg1	Inflammation					105:116	Inflammation	105:116	Inflammation	105:116	Inflammation is a well-characterized critical driver of gastrointestinal cancers.
32369000	11	0	theme	Average	1274:1280	arg1	ANI					1303:1305	ANI	1303:1305	ANI	1303:1305	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) analyses with closely related reference strains indicated that reassociation values were both well below the thresholds of 95-96% and 70 % for species delineation, respectively.
32369000	11	0	theme	Average	1274:1280	arg1	identity					1293:1300	Average nucleotide identity	1274:1300	Average nucleotide identity (ANI)	1274:1306	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) analyses with closely related reference strains indicated that reassociation values were both well below the thresholds of 95-96% and 70 % for species delineation, respectively.
32369000	11	1	theme	species	1492:1498	arg1	delineation					1500:1510	species delineation	1492:1510	species delineation	1492:1510	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) analyses with closely related reference strains indicated that reassociation values were both well below the thresholds of 95-96% and 70 % for species delineation, respectively.
32369000	9	2	theme	DNA	1100:1102	arg1	content					1108:1114	The genomic DNA G+C content	1088:1114	The genomic DNA G+C content	1088:1114	The genomic DNA G+C content was 32.8 mol%.
32369000	9	2	theme	DNA	1100:1102	arg1	%					1128:1128	32.8 mol%	1120:1128	32.8 mol%	1120:1128	The genomic DNA G+C content was 32.8 mol%.
32369000	11	3	theme	DNA-DNA	1320:1326	arg1	hybridization					1328:1340	digital DNA-DNA hybridization	1312:1340	digital DNA-DNA hybridization (dDDH) analyses with closely related reference strains	1312:1395	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) analyses with closely related reference strains indicated that reassociation values were both well below the thresholds of 95-96% and 70 % for species delineation, respectively.
32369000	11	3	theme	DNA-DNA	1320:1326	arg1	dDDH					1343:1346	dDDH	1343:1346	dDDH	1343:1346	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) analyses with closely related reference strains indicated that reassociation values were both well below the thresholds of 95-96% and 70 % for species delineation, respectively.
32369000	4	4	theme	fermentation	571:582	arg1	products					551:558	The end products	543:558	The end products of glucose fermentation	543:582	The end products of glucose fermentation were acetic acid, isobutyric acid and isovaleric acid and a small amount of propionic acid.
32369000	4	4	theme	fermentation	571:582	arg1	acid					596:599	acetic acid	589:599	acetic acid	589:599	The end products of glucose fermentation were acetic acid, isobutyric acid and isovaleric acid and a small amount of propionic acid.
32369000	13	5	theme	=JCM	1668:1671	arg1	1.1530T					1686:1692	=JCM 33482T=GDMCC 1.1530T	1668:1692	=JCM 33482T=GDMCC 1.1530T	1668:1692	The novel isolate ZHW00191T (=JCM 33482T=GDMCC 1.1530T) is proposed as the type strain of the type species Peptacetobacter hominis gen. nov., sp.
32369000	13	5	theme	=JCM	1668:1671	arg1	ZHW00191T					1657:1665	The novel isolate ZHW00191T	1639:1665	The novel isolate ZHW00191T (=JCM 33482T=GDMCC 1.1530T)	1639:1693	The novel isolate ZHW00191T (=JCM 33482T=GDMCC 1.1530T) is proposed as the type strain of the type species Peptacetobacter hominis gen. nov., sp.
32369000	2	6	attach	isolated	328:335	arg1	faeces					348:353	human faeces	342:353	human faeces	342:353	A novel, Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, obligately anaerobic bacterium, designated strain ZHW00191T, was isolated from human faeces and characterized by using a polyphasic taxonomic approach.
32369000	2	6	attach	isolated	328:335	arg2	bacterium					284:292	A novel, Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, obligately anaerobic bacterium	190:292	A novel, Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, obligately anaerobic bacterium	190:292	A novel, Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, obligately anaerobic bacterium, designated strain ZHW00191T, was isolated from human faeces and characterized by using a polyphasic taxonomic approach.
32369000	12	7	theme	gen.	1615:1618	arg1	genus					1592:1596	a novel genus	1584:1596	a novel genus	1584:1596	Based on phenotypic, chemotaxonomic and genetic studies, a novel genus, Peptacetobacter gen. nov., is proposed.
32369000	12	7	theme	gen.	1615:1618	arg1	nov.					1620:1623	Peptacetobacter gen. nov.	1599:1623	Peptacetobacter gen. nov.	1599:1623	Based on phenotypic, chemotaxonomic and genetic studies, a novel genus, Peptacetobacter gen. nov., is proposed.
32369000	4	8	theme	acetic	589:594	arg1	products					551:558	The end products	543:558	The end products of glucose fermentation	543:582	The end products of glucose fermentation were acetic acid, isobutyric acid and isovaleric acid and a small amount of propionic acid.
32369000	4	8	theme	acetic	589:594	arg1	acid					596:599	acetic acid	589:599	acetic acid	589:599	The end products of glucose fermentation were acetic acid, isobutyric acid and isovaleric acid and a small amount of propionic acid.
32369000	9	9	theme	32.8 mol	1120:1127	arg1	content					1108:1114	The genomic DNA G+C content	1088:1114	The genomic DNA G+C content	1088:1114	The genomic DNA G+C content was 32.8 mol%.
32369000	9	9	theme	32.8 mol	1120:1127	arg1	%					1128:1128	32.8 mol%	1120:1128	32.8 mol%	1120:1128	The genomic DNA G+C content was 32.8 mol%.
32369000	4	10	theme	end	547:549	arg1	products					551:558	The end products	543:558	The end products of glucose fermentation	543:582	The end products of glucose fermentation were acetic acid, isobutyric acid and isovaleric acid and a small amount of propionic acid.
32369000	4	10	theme	end	547:549	arg1	acid					596:599	acetic acid	589:599	acetic acid	589:599	The end products of glucose fermentation were acetic acid, isobutyric acid and isovaleric acid and a small amount of propionic acid.
32369000	13	11	theme	novel	1643:1647	arg1	1.1530T					1686:1692	=JCM 33482T=GDMCC 1.1530T	1668:1692	=JCM 33482T=GDMCC 1.1530T	1668:1692	The novel isolate ZHW00191T (=JCM 33482T=GDMCC 1.1530T) is proposed as the type strain of the type species Peptacetobacter hominis gen. nov., sp.
32369000	13	11	theme	novel	1643:1647	arg1	ZHW00191T					1657:1665	The novel isolate ZHW00191T	1639:1665	The novel isolate ZHW00191T (=JCM 33482T=GDMCC 1.1530T)	1639:1693	The novel isolate ZHW00191T (=JCM 33482T=GDMCC 1.1530T) is proposed as the type strain of the type species Peptacetobacter hominis gen. nov., sp.
32369000	13	11	theme	novel	1643:1647	arg1	strain					1719:1724	the type strain	1710:1724	the type strain of the type species Peptacetobacter hominis gen. nov., sp	1710:1782	The novel isolate ZHW00191T (=JCM 33482T=GDMCC 1.1530T) is proposed as the type strain of the type species Peptacetobacter hominis gen. nov., sp.
32369000	1	12	attach	isolated	65:72	arg1	faeces					85:90	human faeces	79:90	human faeces	79:90	nov., isolated from human faeces, and proposal for the reclassification of Clostridium hiranonis within the genus Peptacetobacter.
32369000	1	12	attach	isolated	65:72	arg2	nov.					59:62	nov.	59:62	nov.	59:62	nov., isolated from human faeces, and proposal for the reclassification of Clostridium hiranonis within the genus Peptacetobacter.
32369000	5	13	theme	strain	721:726	arg1	ZHW00191T					728:736	strain ZHW00191T	721:736	strain ZHW00191T	721:736	The dominant cellular fatty acids (>10 %) of strain ZHW00191T were C16 : 0, C18 : 1 ω9с and C18 : 2ω6,9с.
32369000	6	14	theme	polar	786:790	arg1	profile					798:804	Its polar lipid profile	782:804	Its polar lipid profile	782:804	Its polar lipid profile comprised diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids and ten unidentified glycolipids.
32369000	4	15	theme	isovaleric	622:631	arg1	acid					633:636	isovaleric acid	622:636	isovaleric acid	622:636	The end products of glucose fermentation were acetic acid, isobutyric acid and isovaleric acid and a small amount of propionic acid.
32369000	12	16	theme	Peptacetobacter	1599:1613	arg1	genus					1592:1596	a novel genus	1584:1596	a novel genus	1584:1596	Based on phenotypic, chemotaxonomic and genetic studies, a novel genus, Peptacetobacter gen. nov., is proposed.
32369000	12	16	theme	Peptacetobacter	1599:1613	arg1	nov.					1620:1623	Peptacetobacter gen. nov.	1599:1623	Peptacetobacter gen. nov.	1599:1623	Based on phenotypic, chemotaxonomic and genetic studies, a novel genus, Peptacetobacter gen. nov., is proposed.
32369000	11	17	theme	related	1371:1377	arg1	strains					1389:1395	closely related reference strains	1363:1395	closely related reference strains	1363:1395	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) analyses with closely related reference strains indicated that reassociation values were both well below the thresholds of 95-96% and 70 % for species delineation, respectively.
32369000	10	18	theme	gene	1156:1159	arg1	sequence					1161:1168	the 16S rRNA gene sequence	1143:1168	the 16S rRNA gene sequence	1143:1168	Analysis of the 16S rRNA gene sequence indicated that ZHW00191T was most closely related to Clostridium hiranonis TO-931T (95.3 % similarity).
32369000	1	19	theme	hiranonis	146:154	arg1	reclassification					114:129	the reclassification	110:129	the reclassification of Clostridium hiranonis	110:154	nov., isolated from human faeces, and proposal for the reclassification of Clostridium hiranonis within the genus Peptacetobacter.
32369000	4	20	theme	acid	670:673	arg1	acid					633:636	isovaleric acid	622:636	isovaleric acid	622:636	The end products of glucose fermentation were acetic acid, isobutyric acid and isovaleric acid and a small amount of propionic acid.
32369000	4	20	theme	acid	670:673	arg1	products					551:558	The end products	543:558	The end products of glucose fermentation	543:582	The end products of glucose fermentation were acetic acid, isobutyric acid and isovaleric acid and a small amount of propionic acid.
32369000	4	20	theme	acid	670:673	arg1	acid					670:673	propionic acid	660:673	propionic acid	660:673	The end products of glucose fermentation were acetic acid, isobutyric acid and isovaleric acid and a small amount of propionic acid.
32369000	4	20	theme	acid	670:673	arg1	acid					596:599	acetic acid	589:599	acetic acid	589:599	The end products of glucose fermentation were acetic acid, isobutyric acid and isovaleric acid and a small amount of propionic acid.
32369000	4	20	theme	acid	670:673	arg1	acid					613:616	isobutyric acid	602:616	isobutyric acid	602:616	The end products of glucose fermentation were acetic acid, isobutyric acid and isovaleric acid and a small amount of propionic acid.
32369000	4	20	theme	acid	670:673	arg1	amount					650:655	a small amount	642:655	a small amount of propionic acid	642:673	The end products of glucose fermentation were acetic acid, isobutyric acid and isovaleric acid and a small amount of propionic acid.
32369000	13	21	theme	gen.	1770:1773	arg1	sp					1781:1782	sp	1781:1782	sp	1781:1782	The novel isolate ZHW00191T (=JCM 33482T=GDMCC 1.1530T) is proposed as the type strain of the type species Peptacetobacter hominis gen. nov., sp.
32369000	13	21	theme	gen.	1770:1773	arg1	nov.					1775:1778	Peptacetobacter hominis gen. nov.	1746:1778	Peptacetobacter hominis gen. nov.	1746:1778	The novel isolate ZHW00191T (=JCM 33482T=GDMCC 1.1530T) is proposed as the type strain of the type species Peptacetobacter hominis gen. nov., sp.
32369000	15	22	theme	Peptacetobacter	1911:1925	arg1	hiranonis					1862:1870	Clostridium hiranonis	1850:1870	Clostridium hiranonis	1850:1870	Furthermore, it is proposed that Clostridium hiranonis be transferred to this novel genus, as Peptacetobacter hiranonis comb.
32369000	15	22	theme	Peptacetobacter	1911:1925	arg1	comb					1937:1940	Peptacetobacter hiranonis comb	1911:1940	Peptacetobacter hiranonis comb	1911:1940	Furthermore, it is proposed that Clostridium hiranonis be transferred to this novel genus, as Peptacetobacter hiranonis comb.
32369000	2	23	theme	taxonomic	395:403	arg1	approach					405:412	a polyphasic taxonomic approach	382:412	a polyphasic taxonomic approach	382:412	A novel, Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, obligately anaerobic bacterium, designated strain ZHW00191T, was isolated from human faeces and characterized by using a polyphasic taxonomic approach.
32369000	0	24	theme	nov.	49:52	arg1	Characterization					0:15	Characterization	0:15	Characterization of Peptacetobacter hominis gen. nov., sp.	0:57	Characterization of Peptacetobacter hominis gen. nov., sp.
32369000	4	25	theme	small	644:648	arg1	amount					650:655	a small amount	642:655	a small amount of propionic acid	642:673	The end products of glucose fermentation were acetic acid, isobutyric acid and isovaleric acid and a small amount of propionic acid.
32369000	4	25	theme	small	644:648	arg1	acid					670:673	propionic acid	660:673	propionic acid	660:673	The end products of glucose fermentation were acetic acid, isobutyric acid and isovaleric acid and a small amount of propionic acid.
32369000	13	26	theme	type	1733:1736	arg1	species					1738:1744	the type species Peptacetobacter hominis gen. nov., sp	1729:1782	the type species Peptacetobacter hominis gen. nov., sp	1729:1782	The novel isolate ZHW00191T (=JCM 33482T=GDMCC 1.1530T) is proposed as the type strain of the type species Peptacetobacter hominis gen. nov., sp.
32369000	1	27	theme	human	79:83	arg1	faeces					85:90	human faeces	79:90	human faeces	79:90	nov., isolated from human faeces, and proposal for the reclassification of Clostridium hiranonis within the genus Peptacetobacter.
32369000	9	28	theme	G+C	1104:1106	arg1	content					1108:1114	The genomic DNA G+C content	1088:1114	The genomic DNA G+C content	1088:1114	The genomic DNA G+C content was 32.8 mol%.
32369000	9	28	theme	G+C	1104:1106	arg1	%					1128:1128	32.8 mol%	1120:1128	32.8 mol%	1120:1128	The genomic DNA G+C content was 32.8 mol%.
32369000	0	29	theme	Peptacetobacter	20:34	arg1	nov.					49:52	Peptacetobacter hominis gen. nov.	20:52	Peptacetobacter hominis gen. nov.	20:52	Characterization of Peptacetobacter hominis gen. nov., sp.
32369000	0	29	theme	Peptacetobacter	20:34	arg1	sp					55:56	sp	55:56	sp	55:56	Characterization of Peptacetobacter hominis gen. nov., sp.
32369000	13	30	theme	type	1714:1717	arg1	ZHW00191T					1657:1665	The novel isolate ZHW00191T	1639:1665	The novel isolate ZHW00191T (=JCM 33482T=GDMCC 1.1530T)	1639:1693	The novel isolate ZHW00191T (=JCM 33482T=GDMCC 1.1530T) is proposed as the type strain of the type species Peptacetobacter hominis gen. nov., sp.
32369000	13	30	theme	type	1714:1717	arg1	strain					1719:1724	the type strain	1710:1724	the type strain of the type species Peptacetobacter hominis gen. nov., sp	1710:1782	The novel isolate ZHW00191T (=JCM 33482T=GDMCC 1.1530T) is proposed as the type strain of the type species Peptacetobacter hominis gen. nov., sp.
32369000	0	31	theme	gen.	44:47	arg1	nov.					49:52	Peptacetobacter hominis gen. nov.	20:52	Peptacetobacter hominis gen. nov.	20:52	Characterization of Peptacetobacter hominis gen. nov., sp.
32369000	0	31	theme	gen.	44:47	arg1	sp					55:56	sp	55:56	sp	55:56	Characterization of Peptacetobacter hominis gen. nov., sp.
32369000	5	32	theme	cellular	689:696	arg1	acids					704:708	The dominant cellular fatty acids	676:708	The dominant cellular fatty acids (>10 %) of strain ZHW00191T	676:736	The dominant cellular fatty acids (>10 %) of strain ZHW00191T were C16 : 0, C18 : 1 ω9с and C18 : 2ω6,9с.
32369000	5	32	theme	cellular	689:696	arg1	%					715:715	>10 %	711:715	>10 %	711:715	The dominant cellular fatty acids (>10 %) of strain ZHW00191T were C16 : 0, C18 : 1 ω9с and C18 : 2ω6,9с.
32369000	5	32	theme	cellular	689:696	arg1	C16 					743:746	C16 	743:746	C16 	743:746	The dominant cellular fatty acids (>10 %) of strain ZHW00191T were C16 : 0, C18 : 1 ω9с and C18 : 2ω6,9с.
32369000	11	33	theme	%	1486:1486	arg1	thresholds					1458:1467	the thresholds	1454:1467	the thresholds of 95-96% and 70 % for species delineation, respectively	1454:1524	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) analyses with closely related reference strains indicated that reassociation values were both well below the thresholds of 95-96% and 70 % for species delineation, respectively.
32369000	2	34	theme	human	342:346	arg1	faeces					348:353	human faeces	342:353	human faeces	342:353	A novel, Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, obligately anaerobic bacterium, designated strain ZHW00191T, was isolated from human faeces and characterized by using a polyphasic taxonomic approach.
32369000	8	35	theme	meso-2,6-diaminopimelic	1007:1029	arg1	acid					1031:1034	meso-2,6-diaminopimelic acid	1007:1034	meso-2,6-diaminopimelic acid	1007:1034	The cell-wall peptidoglycan contained meso-2,6-diaminopimelic acid, and the whole-cell sugars were ribose and glucose.
32369000	3	36	dep	optimum	444:450	arg1	37-42 °C					453:460	37-42 °C	453:460	37-42 °C	453:460	Growth occurred at 25-45 °C (optimum, 37-42 °C), at pH 5.5-10.0 (optimum, pH 6.5-7.0) and with 0-2 % (w/v) NaCl (optimum, 0 %).
32369000	2	37	theme	novel	192:196	arg1	bacterium					284:292	A novel, Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, obligately anaerobic bacterium	190:292	A novel, Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, obligately anaerobic bacterium	190:292	A novel, Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, obligately anaerobic bacterium, designated strain ZHW00191T, was isolated from human faeces and characterized by using a polyphasic taxonomic approach.
32369000	10	38	theme	Clostridium	1223:1233	arg1	TO-931T					1245:1251	Clostridium hiranonis TO-931T	1223:1251	Clostridium hiranonis TO-931T (95.3 % similarity)	1223:1271	Analysis of the 16S rRNA gene sequence indicated that ZHW00191T was most closely related to Clostridium hiranonis TO-931T (95.3 % similarity).
32369000	10	38	theme	Clostridium	1223:1233	arg1	similarity					1261:1270	95.3 % similarity	1254:1270	95.3 % similarity	1254:1270	Analysis of the 16S rRNA gene sequence indicated that ZHW00191T was most closely related to Clostridium hiranonis TO-931T (95.3 % similarity).
32369000	10	39	theme	sequence	1161:1168	arg1	Analysis					1131:1138	Analysis	1131:1138	Analysis of the 16S rRNA gene sequence	1131:1168	Analysis of the 16S rRNA gene sequence indicated that ZHW00191T was most closely related to Clostridium hiranonis TO-931T (95.3 % similarity).
32369000	11	40	theme	nucleotide	1282:1291	arg1	ANI					1303:1305	ANI	1303:1305	ANI	1303:1305	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) analyses with closely related reference strains indicated that reassociation values were both well below the thresholds of 95-96% and 70 % for species delineation, respectively.
32369000	11	40	theme	nucleotide	1282:1291	arg1	identity					1293:1300	Average nucleotide identity	1274:1300	Average nucleotide identity (ANI)	1274:1306	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) analyses with closely related reference strains indicated that reassociation values were both well below the thresholds of 95-96% and 70 % for species delineation, respectively.
32369000	11	41	theme	reassociation	1412:1424	arg1	values					1426:1431	reassociation values	1412:1431	reassociation values	1412:1431	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) analyses with closely related reference strains indicated that reassociation values were both well below the thresholds of 95-96% and 70 % for species delineation, respectively.
32369000	2	42	dep	novel	192:196	arg1	rod-shaped					220:229	rod-shaped	220:229	rod-shaped	220:229	A novel, Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, obligately anaerobic bacterium, designated strain ZHW00191T, was isolated from human faeces and characterized by using a polyphasic taxonomic approach.
32369000	2	42	dep	novel	192:196	arg1	Gram-stain-positive					199:217	Gram-stain-positive	199:217	Gram-stain-positive	199:217	A novel, Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, obligately anaerobic bacterium, designated strain ZHW00191T, was isolated from human faeces and characterized by using a polyphasic taxonomic approach.
32369000	2	42	dep	novel	192:196	arg1	non-spore-forming					244:260	non-spore-forming	244:260	non-spore-forming	244:260	A novel, Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, obligately anaerobic bacterium, designated strain ZHW00191T, was isolated from human faeces and characterized by using a polyphasic taxonomic approach.
32369000	2	42	dep	novel	192:196	arg1	anaerobic					274:282	anaerobic	274:282	anaerobic	274:282	A novel, Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, obligately anaerobic bacterium, designated strain ZHW00191T, was isolated from human faeces and characterized by using a polyphasic taxonomic approach.
32369000	2	42	dep	novel	192:196	arg1	non-motile					232:241	non-motile	232:241	non-motile	232:241	A novel, Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, obligately anaerobic bacterium, designated strain ZHW00191T, was isolated from human faeces and characterized by using a polyphasic taxonomic approach.
32369000	10	43	theme	95.3	1254:1257	arg1	%					1259:1259	%	1259:1259	%	1259:1259	Analysis of the 16S rRNA gene sequence indicated that ZHW00191T was most closely related to Clostridium hiranonis TO-931T (95.3 % similarity).
32369000	13	44	theme	isolate	1649:1655	arg1	1.1530T					1686:1692	=JCM 33482T=GDMCC 1.1530T	1668:1692	=JCM 33482T=GDMCC 1.1530T	1668:1692	The novel isolate ZHW00191T (=JCM 33482T=GDMCC 1.1530T) is proposed as the type strain of the type species Peptacetobacter hominis gen. nov., sp.
32369000	13	44	theme	isolate	1649:1655	arg1	ZHW00191T					1657:1665	The novel isolate ZHW00191T	1639:1665	The novel isolate ZHW00191T (=JCM 33482T=GDMCC 1.1530T)	1639:1693	The novel isolate ZHW00191T (=JCM 33482T=GDMCC 1.1530T) is proposed as the type strain of the type species Peptacetobacter hominis gen. nov., sp.
32369000	13	44	theme	isolate	1649:1655	arg1	strain					1719:1724	the type strain	1710:1724	the type strain of the type species Peptacetobacter hominis gen. nov., sp	1710:1782	The novel isolate ZHW00191T (=JCM 33482T=GDMCC 1.1530T) is proposed as the type strain of the type species Peptacetobacter hominis gen. nov., sp.
32369000	9	45	theme	genomic	1092:1098	arg1	content					1108:1114	The genomic DNA G+C content	1088:1114	The genomic DNA G+C content	1088:1114	The genomic DNA G+C content was 32.8 mol%.
32369000	9	45	theme	genomic	1092:1098	arg1	%					1128:1128	32.8 mol%	1120:1128	32.8 mol%	1120:1128	The genomic DNA G+C content was 32.8 mol%.
32369000	13	46	theme	Peptacetobacter	1746:1760	arg1	sp					1781:1782	sp	1781:1782	sp	1781:1782	The novel isolate ZHW00191T (=JCM 33482T=GDMCC 1.1530T) is proposed as the type strain of the type species Peptacetobacter hominis gen. nov., sp.
32369000	13	46	theme	Peptacetobacter	1746:1760	arg1	nov.					1775:1778	Peptacetobacter hominis gen. nov.	1746:1778	Peptacetobacter hominis gen. nov.	1746:1778	The novel isolate ZHW00191T (=JCM 33482T=GDMCC 1.1530T) is proposed as the type strain of the type species Peptacetobacter hominis gen. nov., sp.
32369000	3	47	dep	optimum	528:534	arg1	%					539:539	0 %	537:539	0 %	537:539	Growth occurred at 25-45 °C (optimum, 37-42 °C), at pH 5.5-10.0 (optimum, pH 6.5-7.0) and with 0-2 % (w/v) NaCl (optimum, 0 %).
32369000	4	48	theme	glucose	563:569	arg1	fermentation					571:582	glucose fermentation	563:582	glucose fermentation	563:582	The end products of glucose fermentation were acetic acid, isobutyric acid and isovaleric acid and a small amount of propionic acid.
32369000	3	49	theme	w/v	517:519	arg1	optimum					528:534	optimum	528:534	optimum	528:534	Growth occurred at 25-45 °C (optimum, 37-42 °C), at pH 5.5-10.0 (optimum, pH 6.5-7.0) and with 0-2 % (w/v) NaCl (optimum, 0 %).
32369000	3	49	theme	w/v	517:519	arg1	NaCl					522:525	0-2 % (w/v) NaCl	510:525	0-2 % (w/v) NaCl (optimum, 0 %)	510:540	Growth occurred at 25-45 °C (optimum, 37-42 °C), at pH 5.5-10.0 (optimum, pH 6.5-7.0) and with 0-2 % (w/v) NaCl (optimum, 0 %).
32369000	1	50	theme	genus	167:171	arg1	Peptacetobacter					173:187	the genus Peptacetobacter	163:187	the genus Peptacetobacter	163:187	nov., isolated from human faeces, and proposal for the reclassification of Clostridium hiranonis within the genus Peptacetobacter.
32369000	13	51	dep	species	1738:1744	arg1	sp					1781:1782	sp	1781:1782	sp	1781:1782	The novel isolate ZHW00191T (=JCM 33482T=GDMCC 1.1530T) is proposed as the type strain of the type species Peptacetobacter hominis gen. nov., sp.
32369000	13	51	dep	species	1738:1744	arg1	nov.					1775:1778	Peptacetobacter hominis gen. nov.	1746:1778	Peptacetobacter hominis gen. nov.	1746:1778	The novel isolate ZHW00191T (=JCM 33482T=GDMCC 1.1530T) is proposed as the type strain of the type species Peptacetobacter hominis gen. nov., sp.
32369000	14	52	theme	new	1806:1808	arg1	genus					1810:1814	the proposed new genus	1793:1814	the proposed new genus	1793:1814	nov. of the proposed new genus.
32369000	3	53	theme	%	514:514	arg1	optimum					528:534	optimum	528:534	optimum	528:534	Growth occurred at 25-45 °C (optimum, 37-42 °C), at pH 5.5-10.0 (optimum, pH 6.5-7.0) and with 0-2 % (w/v) NaCl (optimum, 0 %).
32369000	3	53	theme	%	514:514	arg1	NaCl					522:525	0-2 % (w/v) NaCl	510:525	0-2 % (w/v) NaCl (optimum, 0 %)	510:540	Growth occurred at 25-45 °C (optimum, 37-42 °C), at pH 5.5-10.0 (optimum, pH 6.5-7.0) and with 0-2 % (w/v) NaCl (optimum, 0 %).
32369000	10	54	theme	hiranonis	1235:1243	arg1	TO-931T					1245:1251	Clostridium hiranonis TO-931T	1223:1251	Clostridium hiranonis TO-931T (95.3 % similarity)	1223:1271	Analysis of the 16S rRNA gene sequence indicated that ZHW00191T was most closely related to Clostridium hiranonis TO-931T (95.3 % similarity).
32369000	10	54	theme	hiranonis	1235:1243	arg1	similarity					1261:1270	95.3 % similarity	1254:1270	95.3 % similarity	1254:1270	Analysis of the 16S rRNA gene sequence indicated that ZHW00191T was most closely related to Clostridium hiranonis TO-931T (95.3 % similarity).
32369000	2	55	theme	strain	306:311	arg1	ZHW00191T					313:321	strain ZHW00191T	306:321	strain ZHW00191T	306:321	A novel, Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, obligately anaerobic bacterium, designated strain ZHW00191T, was isolated from human faeces and characterized by using a polyphasic taxonomic approach.
32369000	13	56	theme	hominis	1762:1768	arg1	sp					1781:1782	sp	1781:1782	sp	1781:1782	The novel isolate ZHW00191T (=JCM 33482T=GDMCC 1.1530T) is proposed as the type strain of the type species Peptacetobacter hominis gen. nov., sp.
32369000	13	56	theme	hominis	1762:1768	arg1	nov.					1775:1778	Peptacetobacter hominis gen. nov.	1746:1778	Peptacetobacter hominis gen. nov.	1746:1778	The novel isolate ZHW00191T (=JCM 33482T=GDMCC 1.1530T) is proposed as the type strain of the type species Peptacetobacter hominis gen. nov., sp.
32369000	5	57	theme	ZHW00191T	728:736	arg1	acids					704:708	The dominant cellular fatty acids	676:708	The dominant cellular fatty acids (>10 %) of strain ZHW00191T	676:736	The dominant cellular fatty acids (>10 %) of strain ZHW00191T were C16 : 0, C18 : 1 ω9с and C18 : 2ω6,9с.
32369000	5	57	theme	ZHW00191T	728:736	arg1	%					715:715	>10 %	711:715	>10 %	711:715	The dominant cellular fatty acids (>10 %) of strain ZHW00191T were C16 : 0, C18 : 1 ω9с and C18 : 2ω6,9с.
32369000	5	57	theme	ZHW00191T	728:736	arg1	C16 					743:746	C16 	743:746	C16 	743:746	The dominant cellular fatty acids (>10 %) of strain ZHW00191T were C16 : 0, C18 : 1 ω9с and C18 : 2ω6,9с.
32369000	6	58	theme	lipid	792:796	arg1	profile					798:804	Its polar lipid profile	782:804	Its polar lipid profile	782:804	Its polar lipid profile comprised diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids and ten unidentified glycolipids.
32369000	11	59	with	analyses	1349:1356	arg1	strains					1389:1395	closely related reference strains	1363:1395	closely related reference strains	1363:1395	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) analyses with closely related reference strains indicated that reassociation values were both well below the thresholds of 95-96% and 70 % for species delineation, respectively.
32369000	7	60	theme	Respiratory	929:939	arg1	quinones					941:948	Respiratory quinones	929:948	Respiratory quinones	929:948	Respiratory quinones were not detected.
32369000	11	61	theme	reference	1379:1387	arg1	strains					1389:1395	closely related reference strains	1363:1395	closely related reference strains	1363:1395	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) analyses with closely related reference strains indicated that reassociation values were both well below the thresholds of 95-96% and 70 % for species delineation, respectively.
32369000	12	62	theme	novel	1586:1590	arg1	genus					1592:1596	a novel genus	1584:1596	a novel genus	1584:1596	Based on phenotypic, chemotaxonomic and genetic studies, a novel genus, Peptacetobacter gen. nov., is proposed.
32369000	12	62	theme	novel	1586:1590	arg1	nov.					1620:1623	Peptacetobacter gen. nov.	1599:1623	Peptacetobacter gen. nov.	1599:1623	Based on phenotypic, chemotaxonomic and genetic studies, a novel genus, Peptacetobacter gen. nov., is proposed.
32369000	6	63	theme	unidentified	903:914	arg1	glycolipids					916:926	ten unidentified glycolipids	899:926	ten unidentified glycolipids	899:926	Its polar lipid profile comprised diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids and ten unidentified glycolipids.
32369000	11	64	theme	 and	1478:1481	arg1	%					1486:1486	95-96% and 70 %	1472:1486	95-96% and 70 % for species delineation, respectively	1472:1524	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) analyses with closely related reference strains indicated that reassociation values were both well below the thresholds of 95-96% and 70 % for species delineation, respectively.
32369000	10	65	theme	16S	1147:1149	arg1	rRNA					1151:1154	the 16S rRNA	1143:1154	the 16S rRNA gene sequence	1143:1168	Analysis of the 16S rRNA gene sequence indicated that ZHW00191T was most closely related to Clostridium hiranonis TO-931T (95.3 % similarity).
32369000	13	66	theme	species	1738:1744	arg1	ZHW00191T					1657:1665	The novel isolate ZHW00191T	1639:1665	The novel isolate ZHW00191T (=JCM 33482T=GDMCC 1.1530T)	1639:1693	The novel isolate ZHW00191T (=JCM 33482T=GDMCC 1.1530T) is proposed as the type strain of the type species Peptacetobacter hominis gen. nov., sp.
32369000	13	66	theme	species	1738:1744	arg1	strain					1719:1724	the type strain	1710:1724	the type strain of the type species Peptacetobacter hominis gen. nov., sp	1710:1782	The novel isolate ZHW00191T (=JCM 33482T=GDMCC 1.1530T) is proposed as the type strain of the type species Peptacetobacter hominis gen. nov., sp.
32369000	1	67	theme	Clostridium	134:144	arg1	hiranonis					146:154	Clostridium hiranonis	134:154	Clostridium hiranonis	134:154	nov., isolated from human faeces, and proposal for the reclassification of Clostridium hiranonis within the genus Peptacetobacter.
32369000	12	68	theme	genetic	1567:1573	arg1	studies					1575:1581	phenotypic, chemotaxonomic and genetic studies	1536:1581	phenotypic, chemotaxonomic and genetic studies	1536:1581	Based on phenotypic, chemotaxonomic and genetic studies, a novel genus, Peptacetobacter gen. nov., is proposed.
32369000	6	69	theme	unidentified	868:879	arg1	phospholipids					881:893	three unidentified phospholipids	862:893	three unidentified phospholipids	862:893	Its polar lipid profile comprised diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids and ten unidentified glycolipids.
32369000	4	70	theme	isobutyric	602:611	arg1	acid					613:616	isobutyric acid	602:616	isobutyric acid	602:616	The end products of glucose fermentation were acetic acid, isobutyric acid and isovaleric acid and a small amount of propionic acid.
32369000	10	71	theme	rRNA	1151:1154	arg1	sequence					1161:1168	the 16S rRNA gene sequence	1143:1168	the 16S rRNA gene sequence	1143:1168	Analysis of the 16S rRNA gene sequence indicated that ZHW00191T was most closely related to Clostridium hiranonis TO-931T (95.3 % similarity).
32369000	4	72	theme	propionic	660:668	arg1	acid					670:673	propionic acid	660:673	propionic acid	660:673	The end products of glucose fermentation were acetic acid, isobutyric acid and isovaleric acid and a small amount of propionic acid.
32369000	11	73	theme	hybridization	1328:1340	arg1	analyses					1349:1356	digital DNA-DNA hybridization (dDDH) analyses	1312:1356	digital DNA-DNA hybridization (dDDH) analyses with closely related reference strains	1312:1395	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) analyses with closely related reference strains indicated that reassociation values were both well below the thresholds of 95-96% and 70 % for species delineation, respectively.
32369000	12	74	theme	phenotypic	1536:1545	arg1	studies					1575:1581	phenotypic, chemotaxonomic and genetic studies	1536:1581	phenotypic, chemotaxonomic and genetic studies	1536:1581	Based on phenotypic, chemotaxonomic and genetic studies, a novel genus, Peptacetobacter gen. nov., is proposed.
32369000	11	75	theme	digital	1312:1318	arg1	hybridization					1328:1340	digital DNA-DNA hybridization	1312:1340	digital DNA-DNA hybridization (dDDH) analyses with closely related reference strains	1312:1395	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) analyses with closely related reference strains indicated that reassociation values were both well below the thresholds of 95-96% and 70 % for species delineation, respectively.
32369000	11	75	theme	digital	1312:1318	arg1	dDDH					1343:1346	dDDH	1343:1346	dDDH	1343:1346	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) analyses with closely related reference strains indicated that reassociation values were both well below the thresholds of 95-96% and 70 % for species delineation, respectively.
32369000	2	76	theme	polyphasic	384:393	arg1	approach					405:412	a polyphasic taxonomic approach	382:412	a polyphasic taxonomic approach	382:412	A novel, Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, obligately anaerobic bacterium, designated strain ZHW00191T, was isolated from human faeces and characterized by using a polyphasic taxonomic approach.
32369000	13	77	theme	33482T=GDMCC	1673:1684	arg1	1.1530T					1686:1692	=JCM 33482T=GDMCC 1.1530T	1668:1692	=JCM 33482T=GDMCC 1.1530T	1668:1692	The novel isolate ZHW00191T (=JCM 33482T=GDMCC 1.1530T) is proposed as the type strain of the type species Peptacetobacter hominis gen. nov., sp.
32369000	13	77	theme	33482T=GDMCC	1673:1684	arg1	ZHW00191T					1657:1665	The novel isolate ZHW00191T	1639:1665	The novel isolate ZHW00191T (=JCM 33482T=GDMCC 1.1530T)	1639:1693	The novel isolate ZHW00191T (=JCM 33482T=GDMCC 1.1530T) is proposed as the type strain of the type species Peptacetobacter hominis gen. nov., sp.
32369000	3	78	dep	optimum	480:486	arg1	pH					489:490	pH 6.5-7.0	489:498	pH 6.5-7.0	489:498	Growth occurred at 25-45 °C (optimum, 37-42 °C), at pH 5.5-10.0 (optimum, pH 6.5-7.0) and with 0-2 % (w/v) NaCl (optimum, 0 %).
32369000	12	79	theme	chemotaxonomic	1548:1561	arg1	studies					1575:1581	phenotypic, chemotaxonomic and genetic studies	1536:1581	phenotypic, chemotaxonomic and genetic studies	1536:1581	Based on phenotypic, chemotaxonomic and genetic studies, a novel genus, Peptacetobacter gen. nov., is proposed.
32369000	0	80	theme	hominis	36:42	arg1	nov.					49:52	Peptacetobacter hominis gen. nov.	20:52	Peptacetobacter hominis gen. nov.	20:52	Characterization of Peptacetobacter hominis gen. nov., sp.
32369000	0	80	theme	hominis	36:42	arg1	sp					55:56	sp	55:56	sp	55:56	Characterization of Peptacetobacter hominis gen. nov., sp.
32369000	8	81	theme	cell-wall	973:981	arg1	peptidoglycan					983:995	The cell-wall peptidoglycan	969:995	The cell-wall peptidoglycan	969:995	The cell-wall peptidoglycan contained meso-2,6-diaminopimelic acid, and the whole-cell sugars were ribose and glucose.
32369000	15	82	theme	novel	1895:1899	arg1	genus					1901:1905	this novel genus	1890:1905	this novel genus	1890:1905	Furthermore, it is proposed that Clostridium hiranonis be transferred to this novel genus, as Peptacetobacter hiranonis comb.
32369000	5	83	theme	dominant	680:687	arg1	acids					704:708	The dominant cellular fatty acids	676:708	The dominant cellular fatty acids (>10 %) of strain ZHW00191T	676:736	The dominant cellular fatty acids (>10 %) of strain ZHW00191T were C16 : 0, C18 : 1 ω9с and C18 : 2ω6,9с.
32369000	5	83	theme	dominant	680:687	arg1	%					715:715	>10 %	711:715	>10 %	711:715	The dominant cellular fatty acids (>10 %) of strain ZHW00191T were C16 : 0, C18 : 1 ω9с and C18 : 2ω6,9с.
32369000	5	83	theme	dominant	680:687	arg1	C16 					743:746	C16 	743:746	C16 	743:746	The dominant cellular fatty acids (>10 %) of strain ZHW00191T were C16 : 0, C18 : 1 ω9с and C18 : 2ω6,9с.
32369000	15	84	theme	Clostridium	1850:1860	arg1	hiranonis					1862:1870	Clostridium hiranonis	1850:1870	Clostridium hiranonis	1850:1870	Furthermore, it is proposed that Clostridium hiranonis be transferred to this novel genus, as Peptacetobacter hiranonis comb.
32369000	15	84	theme	Clostridium	1850:1860	arg1	comb					1937:1940	Peptacetobacter hiranonis comb	1911:1940	Peptacetobacter hiranonis comb	1911:1940	Furthermore, it is proposed that Clostridium hiranonis be transferred to this novel genus, as Peptacetobacter hiranonis comb.
32369000	11	85	theme	70 	1483:1485	arg1	%					1486:1486	95-96% and 70 %	1472:1486	95-96% and 70 % for species delineation, respectively	1472:1524	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) analyses with closely related reference strains indicated that reassociation values were both well below the thresholds of 95-96% and 70 % for species delineation, respectively.
32369000	5	86	theme	fatty	698:702	arg1	acids					704:708	The dominant cellular fatty acids	676:708	The dominant cellular fatty acids (>10 %) of strain ZHW00191T	676:736	The dominant cellular fatty acids (>10 %) of strain ZHW00191T were C16 : 0, C18 : 1 ω9с and C18 : 2ω6,9с.
32369000	5	86	theme	fatty	698:702	arg1	%					715:715	>10 %	711:715	>10 %	711:715	The dominant cellular fatty acids (>10 %) of strain ZHW00191T were C16 : 0, C18 : 1 ω9с and C18 : 2ω6,9с.
32369000	5	86	theme	fatty	698:702	arg1	C16 					743:746	C16 	743:746	C16 	743:746	The dominant cellular fatty acids (>10 %) of strain ZHW00191T were C16 : 0, C18 : 1 ω9с and C18 : 2ω6,9с.
32369000	14	87	theme	genus	1810:1814	arg1	nov.					1785:1788	nov.	1785:1788	nov. of the proposed new genus	1785:1814	nov. of the proposed new genus.
32369000	11	88	theme	%	1477:1477	arg1	%					1486:1486	95-96% and 70 %	1472:1486	95-96% and 70 % for species delineation, respectively	1472:1524	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) analyses with closely related reference strains indicated that reassociation values were both well below the thresholds of 95-96% and 70 % for species delineation, respectively.
32369000	14	89	theme	proposed	1797:1804	arg1	genus					1810:1814	the proposed new genus	1793:1814	the proposed new genus	1793:1814	nov. of the proposed new genus.
32369000	8	90	theme	whole-cell	1045:1054	arg1	sugars					1056:1061	the whole-cell sugars	1041:1061	the whole-cell sugars	1041:1061	The cell-wall peptidoglycan contained meso-2,6-diaminopimelic acid, and the whole-cell sugars were ribose and glucose.
32369000	8	90	theme	whole-cell	1045:1054	arg1	ribose					1068:1073	ribose	1068:1073	ribose	1068:1073	The cell-wall peptidoglycan contained meso-2,6-diaminopimelic acid, and the whole-cell sugars were ribose and glucose.
32369000	15	91	theme	hiranonis	1927:1935	arg1	hiranonis					1862:1870	Clostridium hiranonis	1850:1870	Clostridium hiranonis	1850:1870	Furthermore, it is proposed that Clostridium hiranonis be transferred to this novel genus, as Peptacetobacter hiranonis comb.
32369000	15	91	theme	hiranonis	1927:1935	arg1	comb					1937:1940	Peptacetobacter hiranonis comb	1911:1940	Peptacetobacter hiranonis comb	1911:1940	Furthermore, it is proposed that Clostridium hiranonis be transferred to this novel genus, as Peptacetobacter hiranonis comb.
32369000	11	92	with	identity	1293:1300	arg1	strains					1389:1395	closely related reference strains	1363:1395	closely related reference strains	1363:1395	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) analyses with closely related reference strains indicated that reassociation values were both well below the thresholds of 95-96% and 70 % for species delineation, respectively.
32369000	10	93	theme	%	1259:1259	arg1	TO-931T					1245:1251	Clostridium hiranonis TO-931T	1223:1251	Clostridium hiranonis TO-931T (95.3 % similarity)	1223:1271	Analysis of the 16S rRNA gene sequence indicated that ZHW00191T was most closely related to Clostridium hiranonis TO-931T (95.3 % similarity).
32369000	10	93	theme	%	1259:1259	arg1	similarity					1261:1270	95.3 % similarity	1254:1270	95.3 % similarity	1254:1270	Analysis of the 16S rRNA gene sequence indicated that ZHW00191T was most closely related to Clostridium hiranonis TO-931T (95.3 % similarity).
32369000	8	94	contain	contained	997:1005	arg1	peptidoglycan					983:995	The cell-wall peptidoglycan	969:995	The cell-wall peptidoglycan	969:995	The cell-wall peptidoglycan contained meso-2,6-diaminopimelic acid, and the whole-cell sugars were ribose and glucose.
32369000	8	94	contain	contained	997:1005	arg2	acid					1031:1034	meso-2,6-diaminopimelic acid	1007:1034	meso-2,6-diaminopimelic acid	1007:1034	The cell-wall peptidoglycan contained meso-2,6-diaminopimelic acid, and the whole-cell sugars were ribose and glucose.
31951190	5	0	dep	iso-C15 	902:909	arg1	 0					937:938	 0	937:938	 0	937:938	The predominant isoprenoid quinone was identified as MK-8(H4) and the major fatty acids were iso-C15 : 0, iso-C16:1 h, iso-C16 : 0 and anteiso-C17 : 1ω9c.
31951190	5	0	dep	iso-C15 	902:909	arg1	 1ω9c					957:961	 1ω9c	957:961	iso-C15 : 0, iso-C16:1 h, iso-C16 : 0 and anteiso-C17 : 1ω9c	902:961	The predominant isoprenoid quinone was identified as MK-8(H4) and the major fatty acids were iso-C15 : 0, iso-C16:1 h, iso-C16 : 0 and anteiso-C17 : 1ω9c.
31951190	5	0	dep	iso-C15 	902:909	arg1	anteiso-C17 					944:955	anteiso-C17 	944:955	anteiso-C17 	944:955	The predominant isoprenoid quinone was identified as MK-8(H4) and the major fatty acids were iso-C15 : 0, iso-C16:1 h, iso-C16 : 0 and anteiso-C17 : 1ω9c.
31951190	5	0	dep	iso-C15 	902:909	arg1	 0					911:912	 0	911:912	 0	911:912	The predominant isoprenoid quinone was identified as MK-8(H4) and the major fatty acids were iso-C15 : 0, iso-C16:1 h, iso-C16 : 0 and anteiso-C17 : 1ω9c.
31951190	3	1	theme	w/v	449:451	arg1	NaCl					454:457	0-7.5 % (w/v) NaCl	440:457	0-7.5 % (w/v) NaCl (optimum, 1.0 % NaCl)	440:479	Strain YJ01T was aerobic, non-motile, non-spore-forming cocci with diameters of 1.5-1.9 µm, and was able to grow at 10-37 °C (optimum, 28-30 °C), at pH 4.5-9.0 (optimum, pH 7.0-8.0) and at salinities of 0-7.5 % (w/v) NaCl (optimum, 1.0 % NaCl).
31951190	3	1	theme	w/v	449:451	arg1	optimum					460:466	optimum	460:466	optimum	460:466	Strain YJ01T was aerobic, non-motile, non-spore-forming cocci with diameters of 1.5-1.9 µm, and was able to grow at 10-37 °C (optimum, 28-30 °C), at pH 4.5-9.0 (optimum, pH 7.0-8.0) and at salinities of 0-7.5 % (w/v) NaCl (optimum, 1.0 % NaCl).
31951190	1	2	theme	farming	91:97	arg1	field					99:103	a farming field	89:103	a farming field	89:103	nov., an actinobacterium isolated from soil of a farming field.
31951190	2	3	theme	actinobacterial	128:142	arg1	strain					144:149	A Gram-stain-positive actinobacterial strain	106:149	A Gram-stain-positive actinobacterial strain	106:149	A Gram-stain-positive actinobacterial strain, designated YJ01T, was isolated from a spinach farming field soil at Shinan in Korea.
31951190	3	4	theme	%	446:446	arg1	NaCl					454:457	0-7.5 % (w/v) NaCl	440:457	0-7.5 % (w/v) NaCl (optimum, 1.0 % NaCl)	440:479	Strain YJ01T was aerobic, non-motile, non-spore-forming cocci with diameters of 1.5-1.9 µm, and was able to grow at 10-37 °C (optimum, 28-30 °C), at pH 4.5-9.0 (optimum, pH 7.0-8.0) and at salinities of 0-7.5 % (w/v) NaCl (optimum, 1.0 % NaCl).
31951190	3	4	theme	%	446:446	arg1	optimum					460:466	optimum	460:466	optimum	460:466	Strain YJ01T was aerobic, non-motile, non-spore-forming cocci with diameters of 1.5-1.9 µm, and was able to grow at 10-37 °C (optimum, 28-30 °C), at pH 4.5-9.0 (optimum, pH 7.0-8.0) and at salinities of 0-7.5 % (w/v) NaCl (optimum, 1.0 % NaCl).
31951190	4	5	theme	genera	710:715	arg1	species					699:705	species	699:705	species of genera in the family Intrasporangiaceae showing average nucleotide identity values of 84.2-83.4 %	699:806	Sequence similarities of the 16S rRNA gene of strain YJ01T with closely related relatives were in the range 96.2-92.8 %, and the results of phylogenomic analysis indicated that strain YJ01T was clearly separated from species of genera in the family Intrasporangiaceae showing average nucleotide identity values of 84.2-83.4 %.
31951190	4	6	theme	YJ01T	535:539	arg1	gene					520:523	the 16S rRNA gene	507:523	the 16S rRNA gene of strain YJ01T	507:539	Sequence similarities of the 16S rRNA gene of strain YJ01T with closely related relatives were in the range 96.2-92.8 %, and the results of phylogenomic analysis indicated that strain YJ01T was clearly separated from species of genera in the family Intrasporangiaceae showing average nucleotide identity values of 84.2-83.4 %.
31951190	9	7	theme	novel	1551:1555	arg1	species					1557:1563	a novel species	1549:1563	a novel species	1549:1563	On the basis of phenotypic and chemotaxonomic properties and phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences, strain YJ01T is considered to represent a novel species of a new genus in the family Intrasporangiaceae, for which the name Segeticoccus rhizosphaerae gen. nov. sp.
31951190	6	8	theme	diagnostic	968:977	arg1	ornithine					1017:1025	ornithine	1017:1025	ornithine	1017:1025	The diagnostic diamino acid of the peptidoglycan was ornithine, and the interpeptide bridge was l-Orn-Gly2-d-Glu.
31951190	6	8	theme	diagnostic	968:977	arg1	acid					987:990	The diagnostic diamino acid	964:990	The diagnostic diamino acid of the peptidoglycan	964:1011	The diagnostic diamino acid of the peptidoglycan was ornithine, and the interpeptide bridge was l-Orn-Gly2-d-Glu.
31951190	1	9	attach	isolated	67:74	arg1	soil					81:84	soil	81:84	soil of a farming field	81:103	nov., an actinobacterium isolated from soil of a farming field.
31951190	1	9	attach	isolated	67:74	arg2	actinobacterium					51:65	an actinobacterium	48:65	an actinobacterium isolated from soil of a farming field	48:103	nov., an actinobacterium isolated from soil of a farming field.
31951190	3	10	theme	NaCl	454:457	arg1	salinities					426:435	salinities	426:435	salinities of 0-7.5 % (w/v) NaCl (optimum, 1.0 % NaCl)	426:479	Strain YJ01T was aerobic, non-motile, non-spore-forming cocci with diameters of 1.5-1.9 µm, and was able to grow at 10-37 °C (optimum, 28-30 °C), at pH 4.5-9.0 (optimum, pH 7.0-8.0) and at salinities of 0-7.5 % (w/v) NaCl (optimum, 1.0 % NaCl).
31951190	4	11	theme	%	806:806	arg1	values					786:791	average nucleotide identity values	758:791	average nucleotide identity values of 84.2-83.4 %	758:806	Sequence similarities of the 16S rRNA gene of strain YJ01T with closely related relatives were in the range 96.2-92.8 %, and the results of phylogenomic analysis indicated that strain YJ01T was clearly separated from species of genera in the family Intrasporangiaceae showing average nucleotide identity values of 84.2-83.4 %.
31951190	4	12	theme	rRNA	515:518	arg1	gene					520:523	the 16S rRNA gene	507:523	the 16S rRNA gene of strain YJ01T	507:539	Sequence similarities of the 16S rRNA gene of strain YJ01T with closely related relatives were in the range 96.2-92.8 %, and the results of phylogenomic analysis indicated that strain YJ01T was clearly separated from species of genera in the family Intrasporangiaceae showing average nucleotide identity values of 84.2-83.4 %.
31951190	11	13	theme	Segeticoccus	1711:1722	arg1	rhizosphaerae					1724:1736	Segeticoccus rhizosphaerae	1711:1736	Segeticoccus rhizosphaerae	1711:1736	The type strain of Segeticoccus rhizosphaerae is YJ01T (=KACC 19547T=NBRC 113173T).
31951190	4	14	theme	average	758:764	arg1	values					786:791	average nucleotide identity values	758:791	average nucleotide identity values of 84.2-83.4 %	758:806	Sequence similarities of the 16S rRNA gene of strain YJ01T with closely related relatives were in the range 96.2-92.8 %, and the results of phylogenomic analysis indicated that strain YJ01T was clearly separated from species of genera in the family Intrasporangiaceae showing average nucleotide identity values of 84.2-83.4 %.
31951190	1	15	dep	actinobacterium	51:65	arg1	nov.					42:45	nov.	42:45	nov.	42:45	nov., an actinobacterium isolated from soil of a farming field.
31951190	2	16	theme	farming	198:204	arg1	soil					212:215	a spinach farming field soil	188:215	a spinach farming field soil	188:215	A Gram-stain-positive actinobacterial strain, designated YJ01T, was isolated from a spinach farming field soil at Shinan in Korea.
31951190	1	17	theme	field	99:103	arg1	soil					81:84	soil	81:84	soil of a farming field	81:103	nov., an actinobacterium isolated from soil of a farming field.
31951190	4	18	theme	identity	777:784	arg1	values					786:791	average nucleotide identity values	758:791	average nucleotide identity values of 84.2-83.4 %	758:806	Sequence similarities of the 16S rRNA gene of strain YJ01T with closely related relatives were in the range 96.2-92.8 %, and the results of phylogenomic analysis indicated that strain YJ01T was clearly separated from species of genera in the family Intrasporangiaceae showing average nucleotide identity values of 84.2-83.4 %.
31951190	3	19	with	cocci	293:297	arg1	diameters					304:312	diameters	304:312	diameters of 1.5-1.9 µm	304:326	Strain YJ01T was aerobic, non-motile, non-spore-forming cocci with diameters of 1.5-1.9 µm, and was able to grow at 10-37 °C (optimum, 28-30 °C), at pH 4.5-9.0 (optimum, pH 7.0-8.0) and at salinities of 0-7.5 % (w/v) NaCl (optimum, 1.0 % NaCl).
31951190	4	20	theme	range	584:588	arg1	%					600:600	the range 96.2-92.8 %	580:600	the range 96.2-92.8 %	580:600	Sequence similarities of the 16S rRNA gene of strain YJ01T with closely related relatives were in the range 96.2-92.8 %, and the results of phylogenomic analysis indicated that strain YJ01T was clearly separated from species of genera in the family Intrasporangiaceae showing average nucleotide identity values of 84.2-83.4 %.
31951190	9	21	theme	Segeticoccus	1633:1644	arg1	nov.					1665:1668	the name Segeticoccus rhizosphaerae gen. nov.	1624:1668	the name Segeticoccus rhizosphaerae gen. nov.	1624:1668	On the basis of phenotypic and chemotaxonomic properties and phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences, strain YJ01T is considered to represent a novel species of a new genus in the family Intrasporangiaceae, for which the name Segeticoccus rhizosphaerae gen. nov. sp.
31951190	4	22	from	Intrasporangiaceae	731:748	arg1	species					699:705	species	699:705	species of genera in the family Intrasporangiaceae showing average nucleotide identity values of 84.2-83.4 %	699:806	Sequence similarities of the 16S rRNA gene of strain YJ01T with closely related relatives were in the range 96.2-92.8 %, and the results of phylogenomic analysis indicated that strain YJ01T was clearly separated from species of genera in the family Intrasporangiaceae showing average nucleotide identity values of 84.2-83.4 %.
31951190	9	23	theme	phylogenetic	1412:1423	arg1	analyses					1442:1449	phylogenetic and phylogenomic analyses	1412:1449	phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences	1412:1506	On the basis of phenotypic and chemotaxonomic properties and phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences, strain YJ01T is considered to represent a novel species of a new genus in the family Intrasporangiaceae, for which the name Segeticoccus rhizosphaerae gen. nov. sp.
31951190	9	24	theme	gen.	1660:1663	arg1	nov.					1665:1668	the name Segeticoccus rhizosphaerae gen. nov.	1624:1668	the name Segeticoccus rhizosphaerae gen. nov.	1624:1668	On the basis of phenotypic and chemotaxonomic properties and phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences, strain YJ01T is considered to represent a novel species of a new genus in the family Intrasporangiaceae, for which the name Segeticoccus rhizosphaerae gen. nov. sp.
31951190	4	25	theme	nucleotide	766:775	arg1	values					786:791	average nucleotide identity values	758:791	average nucleotide identity values of 84.2-83.4 %	758:806	Sequence similarities of the 16S rRNA gene of strain YJ01T with closely related relatives were in the range 96.2-92.8 %, and the results of phylogenomic analysis indicated that strain YJ01T was clearly separated from species of genera in the family Intrasporangiaceae showing average nucleotide identity values of 84.2-83.4 %.
31951190	3	26	dep	optimum	460:466	arg1	NaCl					475:478	1.0 % NaCl	469:478	1.0 % NaCl	469:478	Strain YJ01T was aerobic, non-motile, non-spore-forming cocci with diameters of 1.5-1.9 µm, and was able to grow at 10-37 °C (optimum, 28-30 °C), at pH 4.5-9.0 (optimum, pH 7.0-8.0) and at salinities of 0-7.5 % (w/v) NaCl (optimum, 1.0 % NaCl).
31951190	9	27	theme	whole-genome	1485:1496	arg1	sequences					1498:1506	whole-genome sequences	1485:1506	whole-genome sequences	1485:1506	On the basis of phenotypic and chemotaxonomic properties and phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences, strain YJ01T is considered to represent a novel species of a new genus in the family Intrasporangiaceae, for which the name Segeticoccus rhizosphaerae gen. nov. sp.
31951190	4	28	from	genera	710:715	arg1	Intrasporangiaceae					731:748	the family Intrasporangiaceae	720:748	the family Intrasporangiaceae showing average nucleotide identity values of 84.2-83.4 %	720:806	Sequence similarities of the 16S rRNA gene of strain YJ01T with closely related relatives were in the range 96.2-92.8 %, and the results of phylogenomic analysis indicated that strain YJ01T was clearly separated from species of genera in the family Intrasporangiaceae showing average nucleotide identity values of 84.2-83.4 %.
31951190	3	29	theme	1.5-1.9 µm	317:326	arg1	diameters					304:312	diameters	304:312	diameters of 1.5-1.9 µm	304:326	Strain YJ01T was aerobic, non-motile, non-spore-forming cocci with diameters of 1.5-1.9 µm, and was able to grow at 10-37 °C (optimum, 28-30 °C), at pH 4.5-9.0 (optimum, pH 7.0-8.0) and at salinities of 0-7.5 % (w/v) NaCl (optimum, 1.0 % NaCl).
31951190	4	30	theme	phylogenomic	622:633	arg1	analysis					635:642	phylogenomic analysis	622:642	phylogenomic analysis	622:642	Sequence similarities of the 16S rRNA gene of strain YJ01T with closely related relatives were in the range 96.2-92.8 %, and the results of phylogenomic analysis indicated that strain YJ01T was clearly separated from species of genera in the family Intrasporangiaceae showing average nucleotide identity values of 84.2-83.4 %.
31951190	4	31	from	species	699:705	arg1	Intrasporangiaceae					731:748	the family Intrasporangiaceae	720:748	the family Intrasporangiaceae showing average nucleotide identity values of 84.2-83.4 %	720:806	Sequence similarities of the 16S rRNA gene of strain YJ01T with closely related relatives were in the range 96.2-92.8 %, and the results of phylogenomic analysis indicated that strain YJ01T was clearly separated from species of genera in the family Intrasporangiaceae showing average nucleotide identity values of 84.2-83.4 %.
31951190	9	32	theme	rRNA	1461:1464	arg1	sequences					1471:1479	16S rRNA gene sequences	1457:1479	16S rRNA gene sequences	1457:1479	On the basis of phenotypic and chemotaxonomic properties and phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences, strain YJ01T is considered to represent a novel species of a new genus in the family Intrasporangiaceae, for which the name Segeticoccus rhizosphaerae gen. nov. sp.
31951190	7	33	theme	unidentified	1189:1200	arg1	phosphatidylglycolipid					1202:1223	an unidentified phosphatidylglycolipid	1186:1223	an unidentified phosphatidylglycolipid	1186:1223	The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylserine, an unidentified phosphatidylglycolipid, two unidentified phosphoaminolipids and an unidentified phosphoglycoaminolipid.
31951190	7	33	theme	unidentified	1189:1200	arg1	lipids					1094:1099	The major polar lipids	1078:1099	The major polar lipids	1078:1099	The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylserine, an unidentified phosphatidylglycolipid, two unidentified phosphoaminolipids and an unidentified phosphoglycoaminolipid.
31951190	3	34	dep	optimum	363:369	arg1	28-30 °C					372:379	28-30 °C	372:379	28-30 °C	372:379	Strain YJ01T was aerobic, non-motile, non-spore-forming cocci with diameters of 1.5-1.9 µm, and was able to grow at 10-37 °C (optimum, 28-30 °C), at pH 4.5-9.0 (optimum, pH 7.0-8.0) and at salinities of 0-7.5 % (w/v) NaCl (optimum, 1.0 % NaCl).
31951190	0	35	theme	Segeticoccus	0:11	arg1	nov.					32:35	Segeticoccus rhizosphaerae gen. nov.	0:35	Segeticoccus rhizosphaerae gen. nov.	0:35	Segeticoccus rhizosphaerae gen. nov., sp.
31951190	0	36	theme	gen.	27:30	arg1	nov.					32:35	Segeticoccus rhizosphaerae gen. nov.	0:35	Segeticoccus rhizosphaerae gen. nov.	0:35	Segeticoccus rhizosphaerae gen. nov., sp.
31951190	5	37	theme	isoprenoid	825:834	arg1	MK-8					862:865	MK-8	862:865	MK-8(H4)	862:869	The predominant isoprenoid quinone was identified as MK-8(H4) and the major fatty acids were iso-C15 : 0, iso-C16:1 h, iso-C16 : 0 and anteiso-C17 : 1ω9c.
31951190	5	37	theme	isoprenoid	825:834	arg1	quinone					836:842	The predominant isoprenoid quinone	809:842	The predominant isoprenoid quinone	809:842	The predominant isoprenoid quinone was identified as MK-8(H4) and the major fatty acids were iso-C15 : 0, iso-C16:1 h, iso-C16 : 0 and anteiso-C17 : 1ω9c.
31951190	8	38	theme	G+C	1310:1312	arg1	%					1348:1348	70.1 mol%	1340:1348	70.1 mol%	1340:1348	The G+C content of the genome was 70.1 mol%.
31951190	8	38	theme	G+C	1310:1312	arg1	content					1314:1320	The G+C content	1306:1320	The G+C content of the genome	1306:1334	The G+C content of the genome was 70.1 mol%.
31951190	5	39	theme	fatty	885:889	arg1	iso-C15 					902:909	iso-C15 	902:909	iso-C15 	902:909	The predominant isoprenoid quinone was identified as MK-8(H4) and the major fatty acids were iso-C15 : 0, iso-C16:1 h, iso-C16 : 0 and anteiso-C17 : 1ω9c.
31951190	5	39	theme	fatty	885:889	arg1	acids					891:895	the major fatty acids	875:895	the major fatty acids	875:895	The predominant isoprenoid quinone was identified as MK-8(H4) and the major fatty acids were iso-C15 : 0, iso-C16:1 h, iso-C16 : 0 and anteiso-C17 : 1ω9c.
31951190	8	40	theme	genome	1329:1334	arg1	%					1348:1348	70.1 mol%	1340:1348	70.1 mol%	1340:1348	The G+C content of the genome was 70.1 mol%.
31951190	8	40	theme	genome	1329:1334	arg1	content					1314:1320	The G+C content	1306:1320	The G+C content of the genome	1306:1334	The G+C content of the genome was 70.1 mol%.
31951190	3	41	dep	optimum	398:404	arg1	7.0-8.0					410:416	pH 7.0-8.0	407:416	pH 7.0-8.0	407:416	Strain YJ01T was aerobic, non-motile, non-spore-forming cocci with diameters of 1.5-1.9 µm, and was able to grow at 10-37 °C (optimum, 28-30 °C), at pH 4.5-9.0 (optimum, pH 7.0-8.0) and at salinities of 0-7.5 % (w/v) NaCl (optimum, 1.0 % NaCl).
31951190	7	42	theme	polar	1088:1092	arg1	phosphoaminolipids					1243:1260	two unidentified phosphoaminolipids	1226:1260	two unidentified phosphoaminolipids	1226:1260	The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylserine, an unidentified phosphatidylglycolipid, two unidentified phosphoaminolipids and an unidentified phosphoglycoaminolipid.
31951190	7	42	theme	polar	1088:1092	arg1	diphosphatidylglycerol					1120:1141	diphosphatidylglycerol	1120:1141	diphosphatidylglycerol	1120:1141	The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylserine, an unidentified phosphatidylglycolipid, two unidentified phosphoaminolipids and an unidentified phosphoglycoaminolipid.
31951190	7	42	theme	polar	1088:1092	arg1	phosphatidylglycerol					1144:1163	phosphatidylglycerol	1144:1163	phosphatidylglycerol	1144:1163	The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylserine, an unidentified phosphatidylglycolipid, two unidentified phosphoaminolipids and an unidentified phosphoglycoaminolipid.
31951190	7	42	theme	polar	1088:1092	arg1	phosphatidylglycolipid					1202:1223	an unidentified phosphatidylglycolipid	1186:1223	an unidentified phosphatidylglycolipid	1186:1223	The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylserine, an unidentified phosphatidylglycolipid, two unidentified phosphoaminolipids and an unidentified phosphoglycoaminolipid.
31951190	7	42	theme	polar	1088:1092	arg1	lipids					1094:1099	The major polar lipids	1078:1099	The major polar lipids	1078:1099	The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylserine, an unidentified phosphatidylglycolipid, two unidentified phosphoaminolipids and an unidentified phosphoglycoaminolipid.
31951190	7	42	theme	polar	1088:1092	arg1	phosphoglycoaminolipid					1282:1303	an unidentified phosphoglycoaminolipid	1266:1303	an unidentified phosphoglycoaminolipid	1266:1303	The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylserine, an unidentified phosphatidylglycolipid, two unidentified phosphoaminolipids and an unidentified phosphoglycoaminolipid.
31951190	7	42	theme	polar	1088:1092	arg1	phosphatidylserine					1166:1183	phosphatidylserine	1166:1183	phosphatidylserine	1166:1183	The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylserine, an unidentified phosphatidylglycolipid, two unidentified phosphoaminolipids and an unidentified phosphoglycoaminolipid.
31951190	4	43	theme	Sequence	482:489	arg1	similarities					491:502	Sequence similarities	482:502	Sequence similarities of the 16S rRNA gene of strain YJ01T with closely related relatives	482:570	Sequence similarities of the 16S rRNA gene of strain YJ01T with closely related relatives were in the range 96.2-92.8 %, and the results of phylogenomic analysis indicated that strain YJ01T was clearly separated from species of genera in the family Intrasporangiaceae showing average nucleotide identity values of 84.2-83.4 %.
31951190	11	44	theme	19547T=NBRC	1754:1764	arg1	113173T					1766:1772	=KACC 19547T=NBRC 113173T	1748:1772	=KACC 19547T=NBRC 113173T	1748:1772	The type strain of Segeticoccus rhizosphaerae is YJ01T (=KACC 19547T=NBRC 113173T).
31951190	11	44	theme	19547T=NBRC	1754:1764	arg1	YJ01T					1741:1745	YJ01T	1741:1745	YJ01T (=KACC 19547T=NBRC 113173T)	1741:1773	The type strain of Segeticoccus rhizosphaerae is YJ01T (=KACC 19547T=NBRC 113173T).
31951190	9	45	theme	genus	1574:1578	arg1	species					1557:1563	a novel species	1549:1563	a novel species	1549:1563	On the basis of phenotypic and chemotaxonomic properties and phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences, strain YJ01T is considered to represent a novel species of a new genus in the family Intrasporangiaceae, for which the name Segeticoccus rhizosphaerae gen. nov. sp.
31951190	0	46	dep	sp	38:39	arg1	nov.					32:35	Segeticoccus rhizosphaerae gen. nov.	0:35	Segeticoccus rhizosphaerae gen. nov.	0:35	Segeticoccus rhizosphaerae gen. nov., sp.
31951190	4	47	theme	family	724:729	arg1	Intrasporangiaceae					731:748	the family Intrasporangiaceae	720:748	the family Intrasporangiaceae showing average nucleotide identity values of 84.2-83.4 %	720:806	Sequence similarities of the 16S rRNA gene of strain YJ01T with closely related relatives were in the range 96.2-92.8 %, and the results of phylogenomic analysis indicated that strain YJ01T was clearly separated from species of genera in the family Intrasporangiaceae showing average nucleotide identity values of 84.2-83.4 %.
31951190	6	48	theme	peptidoglycan	999:1011	arg1	ornithine					1017:1025	ornithine	1017:1025	ornithine	1017:1025	The diagnostic diamino acid of the peptidoglycan was ornithine, and the interpeptide bridge was l-Orn-Gly2-d-Glu.
31951190	6	48	theme	peptidoglycan	999:1011	arg1	acid					987:990	The diagnostic diamino acid	964:990	The diagnostic diamino acid of the peptidoglycan	964:1011	The diagnostic diamino acid of the peptidoglycan was ornithine, and the interpeptide bridge was l-Orn-Gly2-d-Glu.
31951190	3	49	theme	0-7.5 	440:445	arg1	NaCl					454:457	0-7.5 % (w/v) NaCl	440:457	0-7.5 % (w/v) NaCl (optimum, 1.0 % NaCl)	440:479	Strain YJ01T was aerobic, non-motile, non-spore-forming cocci with diameters of 1.5-1.9 µm, and was able to grow at 10-37 °C (optimum, 28-30 °C), at pH 4.5-9.0 (optimum, pH 7.0-8.0) and at salinities of 0-7.5 % (w/v) NaCl (optimum, 1.0 % NaCl).
31951190	3	49	theme	0-7.5 	440:445	arg1	optimum					460:466	optimum	460:466	optimum	460:466	Strain YJ01T was aerobic, non-motile, non-spore-forming cocci with diameters of 1.5-1.9 µm, and was able to grow at 10-37 °C (optimum, 28-30 °C), at pH 4.5-9.0 (optimum, pH 7.0-8.0) and at salinities of 0-7.5 % (w/v) NaCl (optimum, 1.0 % NaCl).
31951190	9	50	theme	new	1570:1572	arg1	genus					1574:1578	a new genus	1568:1578	a new genus in the family Intrasporangiaceae	1568:1611	On the basis of phenotypic and chemotaxonomic properties and phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences, strain YJ01T is considered to represent a novel species of a new genus in the family Intrasporangiaceae, for which the name Segeticoccus rhizosphaerae gen. nov. sp.
31951190	4	51	theme	gene	520:523	arg1	similarities					491:502	Sequence similarities	482:502	Sequence similarities of the 16S rRNA gene of strain YJ01T with closely related relatives	482:570	Sequence similarities of the 16S rRNA gene of strain YJ01T with closely related relatives were in the range 96.2-92.8 %, and the results of phylogenomic analysis indicated that strain YJ01T was clearly separated from species of genera in the family Intrasporangiaceae showing average nucleotide identity values of 84.2-83.4 %.
31951190	2	52	theme	Gram-stain-positive	108:126	arg1	strain					144:149	A Gram-stain-positive actinobacterial strain	106:149	A Gram-stain-positive actinobacterial strain	106:149	A Gram-stain-positive actinobacterial strain, designated YJ01T, was isolated from a spinach farming field soil at Shinan in Korea.
31951190	9	53	from	genus	1574:1578	arg1	Intrasporangiaceae					1594:1611	the family Intrasporangiaceae	1583:1611	the family Intrasporangiaceae	1583:1611	On the basis of phenotypic and chemotaxonomic properties and phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences, strain YJ01T is considered to represent a novel species of a new genus in the family Intrasporangiaceae, for which the name Segeticoccus rhizosphaerae gen. nov. sp.
31951190	6	54	theme	diamino	979:985	arg1	ornithine					1017:1025	ornithine	1017:1025	ornithine	1017:1025	The diagnostic diamino acid of the peptidoglycan was ornithine, and the interpeptide bridge was l-Orn-Gly2-d-Glu.
31951190	6	54	theme	diamino	979:985	arg1	acid					987:990	The diagnostic diamino acid	964:990	The diagnostic diamino acid of the peptidoglycan	964:1011	The diagnostic diamino acid of the peptidoglycan was ornithine, and the interpeptide bridge was l-Orn-Gly2-d-Glu.
31951190	4	55	theme	strain	528:533	arg1	YJ01T					535:539	strain YJ01T	528:539	strain YJ01T	528:539	Sequence similarities of the 16S rRNA gene of strain YJ01T with closely related relatives were in the range 96.2-92.8 %, and the results of phylogenomic analysis indicated that strain YJ01T was clearly separated from species of genera in the family Intrasporangiaceae showing average nucleotide identity values of 84.2-83.4 %.
31951190	11	56	theme	type	1696:1699	arg1	YJ01T					1741:1745	YJ01T	1741:1745	YJ01T (=KACC 19547T=NBRC 113173T)	1741:1773	The type strain of Segeticoccus rhizosphaerae is YJ01T (=KACC 19547T=NBRC 113173T).
31951190	11	56	theme	type	1696:1699	arg1	strain					1701:1706	The type strain	1692:1706	The type strain of Segeticoccus rhizosphaerae	1692:1736	The type strain of Segeticoccus rhizosphaerae is YJ01T (=KACC 19547T=NBRC 113173T).
31951190	9	57	theme	family	1587:1592	arg1	Intrasporangiaceae					1594:1611	the family Intrasporangiaceae	1583:1611	the family Intrasporangiaceae	1583:1611	On the basis of phenotypic and chemotaxonomic properties and phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences, strain YJ01T is considered to represent a novel species of a new genus in the family Intrasporangiaceae, for which the name Segeticoccus rhizosphaerae gen. nov. sp.
31951190	8	58	theme	70.1 mol	1340:1347	arg1	%					1348:1348	70.1 mol%	1340:1348	70.1 mol%	1340:1348	The G+C content of the genome was 70.1 mol%.
31951190	8	58	theme	70.1 mol	1340:1347	arg1	content					1314:1320	The G+C content	1306:1320	The G+C content of the genome	1306:1334	The G+C content of the genome was 70.1 mol%.
31951190	9	59	theme	phylogenomic	1429:1440	arg1	analyses					1442:1449	phylogenetic and phylogenomic analyses	1412:1449	phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences	1412:1506	On the basis of phenotypic and chemotaxonomic properties and phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences, strain YJ01T is considered to represent a novel species of a new genus in the family Intrasporangiaceae, for which the name Segeticoccus rhizosphaerae gen. nov. sp.
31951190	5	60	theme	major	879:883	arg1	iso-C15 					902:909	iso-C15 	902:909	iso-C15 	902:909	The predominant isoprenoid quinone was identified as MK-8(H4) and the major fatty acids were iso-C15 : 0, iso-C16:1 h, iso-C16 : 0 and anteiso-C17 : 1ω9c.
31951190	5	60	theme	major	879:883	arg1	acids					891:895	the major fatty acids	875:895	the major fatty acids	875:895	The predominant isoprenoid quinone was identified as MK-8(H4) and the major fatty acids were iso-C15 : 0, iso-C16:1 h, iso-C16 : 0 and anteiso-C17 : 1ω9c.
31951190	9	61	dep	Segeticoccus	1633:1644	arg1	rhizosphaerae					1646:1658	rhizosphaerae	1646:1658	rhizosphaerae	1646:1658	On the basis of phenotypic and chemotaxonomic properties and phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences, strain YJ01T is considered to represent a novel species of a new genus in the family Intrasporangiaceae, for which the name Segeticoccus rhizosphaerae gen. nov. sp.
31951190	4	62	with	similarities	491:502	arg1	relatives					562:570	closely related relatives	546:570	closely related relatives	546:570	Sequence similarities of the 16S rRNA gene of strain YJ01T with closely related relatives were in the range 96.2-92.8 %, and the results of phylogenomic analysis indicated that strain YJ01T was clearly separated from species of genera in the family Intrasporangiaceae showing average nucleotide identity values of 84.2-83.4 %.
31951190	9	63	from	species	1557:1563	arg1	Intrasporangiaceae					1594:1611	the family Intrasporangiaceae	1583:1611	the family Intrasporangiaceae	1583:1611	On the basis of phenotypic and chemotaxonomic properties and phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences, strain YJ01T is considered to represent a novel species of a new genus in the family Intrasporangiaceae, for which the name Segeticoccus rhizosphaerae gen. nov. sp.
31951190	7	64	theme	unidentified	1269:1280	arg1	phosphoglycoaminolipid					1282:1303	an unidentified phosphoglycoaminolipid	1266:1303	an unidentified phosphoglycoaminolipid	1266:1303	The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylserine, an unidentified phosphatidylglycolipid, two unidentified phosphoaminolipids and an unidentified phosphoglycoaminolipid.
31951190	7	64	theme	unidentified	1269:1280	arg1	lipids					1094:1099	The major polar lipids	1078:1099	The major polar lipids	1078:1099	The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylserine, an unidentified phosphatidylglycolipid, two unidentified phosphoaminolipids and an unidentified phosphoglycoaminolipid.
31951190	4	65	theme	16S	511:513	arg1	gene					520:523	the 16S rRNA gene	507:523	the 16S rRNA gene of strain YJ01T	507:539	Sequence similarities of the 16S rRNA gene of strain YJ01T with closely related relatives were in the range 96.2-92.8 %, and the results of phylogenomic analysis indicated that strain YJ01T was clearly separated from species of genera in the family Intrasporangiaceae showing average nucleotide identity values of 84.2-83.4 %.
31951190	11	66	theme	rhizosphaerae	1724:1736	arg1	YJ01T					1741:1745	YJ01T	1741:1745	YJ01T (=KACC 19547T=NBRC 113173T)	1741:1773	The type strain of Segeticoccus rhizosphaerae is YJ01T (=KACC 19547T=NBRC 113173T).
31951190	11	66	theme	rhizosphaerae	1724:1736	arg1	strain					1701:1706	The type strain	1692:1706	The type strain of Segeticoccus rhizosphaerae	1692:1736	The type strain of Segeticoccus rhizosphaerae is YJ01T (=KACC 19547T=NBRC 113173T).
31951190	2	67	theme	field	206:210	arg1	soil					212:215	a spinach farming field soil	188:215	a spinach farming field soil	188:215	A Gram-stain-positive actinobacterial strain, designated YJ01T, was isolated from a spinach farming field soil at Shinan in Korea.
31951190	9	68	theme	phenotypic	1367:1376	arg1	properties					1397:1406	phenotypic and chemotaxonomic properties	1367:1406	phenotypic and chemotaxonomic properties	1367:1406	On the basis of phenotypic and chemotaxonomic properties and phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences, strain YJ01T is considered to represent a novel species of a new genus in the family Intrasporangiaceae, for which the name Segeticoccus rhizosphaerae gen. nov. sp.
31951190	3	69	theme	non-spore-forming	275:291	arg1	cocci					293:297	non-spore-forming cocci	275:297	non-spore-forming cocci with diameters of 1.5-1.9 µm	275:326	Strain YJ01T was aerobic, non-motile, non-spore-forming cocci with diameters of 1.5-1.9 µm, and was able to grow at 10-37 °C (optimum, 28-30 °C), at pH 4.5-9.0 (optimum, pH 7.0-8.0) and at salinities of 0-7.5 % (w/v) NaCl (optimum, 1.0 % NaCl).
31951190	3	69	theme	non-spore-forming	275:291	arg1	aerobic					254:260	aerobic	254:260	aerobic	254:260	Strain YJ01T was aerobic, non-motile, non-spore-forming cocci with diameters of 1.5-1.9 µm, and was able to grow at 10-37 °C (optimum, 28-30 °C), at pH 4.5-9.0 (optimum, pH 7.0-8.0) and at salinities of 0-7.5 % (w/v) NaCl (optimum, 1.0 % NaCl).
31951190	6	70	theme	interpeptide	1036:1047	arg1	bridge					1049:1054	the interpeptide bridge	1032:1054	the interpeptide bridge	1032:1054	The diagnostic diamino acid of the peptidoglycan was ornithine, and the interpeptide bridge was l-Orn-Gly2-d-Glu.
31951190	6	70	theme	interpeptide	1036:1047	arg1	l-Orn-Gly2-d-Glu					1060:1075	l-Orn-Gly2-d-Glu	1060:1075	l-Orn-Gly2-d-Glu	1060:1075	The diagnostic diamino acid of the peptidoglycan was ornithine, and the interpeptide bridge was l-Orn-Gly2-d-Glu.
31951190	9	71	dep	sp	1670:1671	arg1	nov.					1665:1668	the name Segeticoccus rhizosphaerae gen. nov.	1624:1668	the name Segeticoccus rhizosphaerae gen. nov.	1624:1668	On the basis of phenotypic and chemotaxonomic properties and phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences, strain YJ01T is considered to represent a novel species of a new genus in the family Intrasporangiaceae, for which the name Segeticoccus rhizosphaerae gen. nov. sp.
31951190	9	72	theme	chemotaxonomic	1382:1395	arg1	properties					1397:1406	phenotypic and chemotaxonomic properties	1367:1406	phenotypic and chemotaxonomic properties	1367:1406	On the basis of phenotypic and chemotaxonomic properties and phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences, strain YJ01T is considered to represent a novel species of a new genus in the family Intrasporangiaceae, for which the name Segeticoccus rhizosphaerae gen. nov. sp.
31951190	9	73	theme	name	1628:1631	arg1	nov.					1665:1668	the name Segeticoccus rhizosphaerae gen. nov.	1624:1668	the name Segeticoccus rhizosphaerae gen. nov.	1624:1668	On the basis of phenotypic and chemotaxonomic properties and phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences, strain YJ01T is considered to represent a novel species of a new genus in the family Intrasporangiaceae, for which the name Segeticoccus rhizosphaerae gen. nov. sp.
31951190	7	74	theme	unidentified	1230:1241	arg1	lipids					1094:1099	The major polar lipids	1078:1099	The major polar lipids	1078:1099	The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylserine, an unidentified phosphatidylglycolipid, two unidentified phosphoaminolipids and an unidentified phosphoglycoaminolipid.
31951190	7	74	theme	unidentified	1230:1241	arg1	phosphoaminolipids					1243:1260	two unidentified phosphoaminolipids	1226:1260	two unidentified phosphoaminolipids	1226:1260	The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylserine, an unidentified phosphatidylglycolipid, two unidentified phosphoaminolipids and an unidentified phosphoglycoaminolipid.
31951190	9	75	from	Intrasporangiaceae	1594:1611	arg1	species					1557:1563	a novel species	1549:1563	a novel species	1549:1563	On the basis of phenotypic and chemotaxonomic properties and phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences, strain YJ01T is considered to represent a novel species of a new genus in the family Intrasporangiaceae, for which the name Segeticoccus rhizosphaerae gen. nov. sp.
31951190	4	76	theme	related	554:560	arg1	relatives					562:570	closely related relatives	546:570	closely related relatives	546:570	Sequence similarities of the 16S rRNA gene of strain YJ01T with closely related relatives were in the range 96.2-92.8 %, and the results of phylogenomic analysis indicated that strain YJ01T was clearly separated from species of genera in the family Intrasporangiaceae showing average nucleotide identity values of 84.2-83.4 %.
31951190	9	77	dep	properties	1397:1406	arg1	the					1354:1356	the	1354:1356	the	1354:1356	On the basis of phenotypic and chemotaxonomic properties and phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences, strain YJ01T is considered to represent a novel species of a new genus in the family Intrasporangiaceae, for which the name Segeticoccus rhizosphaerae gen. nov. sp.
31951190	9	77	dep	properties	1397:1406	arg1	basis					1358:1362	basis	1358:1362	basis	1358:1362	On the basis of phenotypic and chemotaxonomic properties and phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences, strain YJ01T is considered to represent a novel species of a new genus in the family Intrasporangiaceae, for which the name Segeticoccus rhizosphaerae gen. nov. sp.
31951190	9	78	theme	16S	1457:1459	arg1	sequences					1471:1479	16S rRNA gene sequences	1457:1479	16S rRNA gene sequences	1457:1479	On the basis of phenotypic and chemotaxonomic properties and phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences, strain YJ01T is considered to represent a novel species of a new genus in the family Intrasporangiaceae, for which the name Segeticoccus rhizosphaerae gen. nov. sp.
31951190	0	79	theme	rhizosphaerae	13:25	arg1	nov.					32:35	Segeticoccus rhizosphaerae gen. nov.	0:35	Segeticoccus rhizosphaerae gen. nov.	0:35	Segeticoccus rhizosphaerae gen. nov., sp.
31951190	9	80	theme	gene	1466:1469	arg1	sequences					1471:1479	16S rRNA gene sequences	1457:1479	16S rRNA gene sequences	1457:1479	On the basis of phenotypic and chemotaxonomic properties and phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences, strain YJ01T is considered to represent a novel species of a new genus in the family Intrasporangiaceae, for which the name Segeticoccus rhizosphaerae gen. nov. sp.
31951190	2	81	attach	isolated	174:181	arg1	soil					212:215	a spinach farming field soil	188:215	a spinach farming field soil	188:215	A Gram-stain-positive actinobacterial strain, designated YJ01T, was isolated from a spinach farming field soil at Shinan in Korea.
31951190	2	81	attach	isolated	174:181	arg2	strain					144:149	A Gram-stain-positive actinobacterial strain	106:149	A Gram-stain-positive actinobacterial strain	106:149	A Gram-stain-positive actinobacterial strain, designated YJ01T, was isolated from a spinach farming field soil at Shinan in Korea.
31951190	3	82	theme	Strain	237:242	arg1	YJ01T					244:248	Strain YJ01T	237:248	Strain YJ01T	237:248	Strain YJ01T was aerobic, non-motile, non-spore-forming cocci with diameters of 1.5-1.9 µm, and was able to grow at 10-37 °C (optimum, 28-30 °C), at pH 4.5-9.0 (optimum, pH 7.0-8.0) and at salinities of 0-7.5 % (w/v) NaCl (optimum, 1.0 % NaCl).
31951190	4	83	theme	strain	659:664	arg1	YJ01T					666:670	strain YJ01T	659:670	strain YJ01T	659:670	Sequence similarities of the 16S rRNA gene of strain YJ01T with closely related relatives were in the range 96.2-92.8 %, and the results of phylogenomic analysis indicated that strain YJ01T was clearly separated from species of genera in the family Intrasporangiaceae showing average nucleotide identity values of 84.2-83.4 %.
31951190	5	84	theme	predominant	813:823	arg1	MK-8					862:865	MK-8	862:865	MK-8(H4)	862:869	The predominant isoprenoid quinone was identified as MK-8(H4) and the major fatty acids were iso-C15 : 0, iso-C16:1 h, iso-C16 : 0 and anteiso-C17 : 1ω9c.
31951190	5	84	theme	predominant	813:823	arg1	quinone					836:842	The predominant isoprenoid quinone	809:842	The predominant isoprenoid quinone	809:842	The predominant isoprenoid quinone was identified as MK-8(H4) and the major fatty acids were iso-C15 : 0, iso-C16:1 h, iso-C16 : 0 and anteiso-C17 : 1ω9c.
31951190	3	85	theme	%	473:473	arg1	NaCl					475:478	1.0 % NaCl	469:478	1.0 % NaCl	469:478	Strain YJ01T was aerobic, non-motile, non-spore-forming cocci with diameters of 1.5-1.9 µm, and was able to grow at 10-37 °C (optimum, 28-30 °C), at pH 4.5-9.0 (optimum, pH 7.0-8.0) and at salinities of 0-7.5 % (w/v) NaCl (optimum, 1.0 % NaCl).
31951190	9	86	theme	strain	1509:1514	arg1	YJ01T					1516:1520	strain YJ01T	1509:1520	strain YJ01T	1509:1520	On the basis of phenotypic and chemotaxonomic properties and phylogenetic and phylogenomic analyses using 16S rRNA gene sequences and whole-genome sequences, strain YJ01T is considered to represent a novel species of a new genus in the family Intrasporangiaceae, for which the name Segeticoccus rhizosphaerae gen. nov. sp.
31951190	2	87	theme	spinach	190:196	arg1	soil					212:215	a spinach farming field soil	188:215	a spinach farming field soil	188:215	A Gram-stain-positive actinobacterial strain, designated YJ01T, was isolated from a spinach farming field soil at Shinan in Korea.
31951190	11	88	theme	=KACC	1748:1752	arg1	113173T					1766:1772	=KACC 19547T=NBRC 113173T	1748:1772	=KACC 19547T=NBRC 113173T	1748:1772	The type strain of Segeticoccus rhizosphaerae is YJ01T (=KACC 19547T=NBRC 113173T).
31951190	11	88	theme	=KACC	1748:1752	arg1	YJ01T					1741:1745	YJ01T	1741:1745	YJ01T (=KACC 19547T=NBRC 113173T)	1741:1773	The type strain of Segeticoccus rhizosphaerae is YJ01T (=KACC 19547T=NBRC 113173T).
31951190	4	89	theme	analysis	635:642	arg1	results					611:617	the results	607:617	the results of phylogenomic analysis	607:642	Sequence similarities of the 16S rRNA gene of strain YJ01T with closely related relatives were in the range 96.2-92.8 %, and the results of phylogenomic analysis indicated that strain YJ01T was clearly separated from species of genera in the family Intrasporangiaceae showing average nucleotide identity values of 84.2-83.4 %.
31951190	3	90	theme	pH	407:408	arg1	7.0-8.0					410:416	pH 7.0-8.0	407:416	pH 7.0-8.0	407:416	Strain YJ01T was aerobic, non-motile, non-spore-forming cocci with diameters of 1.5-1.9 µm, and was able to grow at 10-37 °C (optimum, 28-30 °C), at pH 4.5-9.0 (optimum, pH 7.0-8.0) and at salinities of 0-7.5 % (w/v) NaCl (optimum, 1.0 % NaCl).
31951190	7	91	theme	major	1082:1086	arg1	phosphoaminolipids					1243:1260	two unidentified phosphoaminolipids	1226:1260	two unidentified phosphoaminolipids	1226:1260	The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylserine, an unidentified phosphatidylglycolipid, two unidentified phosphoaminolipids and an unidentified phosphoglycoaminolipid.
31951190	7	91	theme	major	1082:1086	arg1	diphosphatidylglycerol					1120:1141	diphosphatidylglycerol	1120:1141	diphosphatidylglycerol	1120:1141	The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylserine, an unidentified phosphatidylglycolipid, two unidentified phosphoaminolipids and an unidentified phosphoglycoaminolipid.
31951190	7	91	theme	major	1082:1086	arg1	phosphatidylglycerol					1144:1163	phosphatidylglycerol	1144:1163	phosphatidylglycerol	1144:1163	The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylserine, an unidentified phosphatidylglycolipid, two unidentified phosphoaminolipids and an unidentified phosphoglycoaminolipid.
31951190	7	91	theme	major	1082:1086	arg1	phosphatidylglycolipid					1202:1223	an unidentified phosphatidylglycolipid	1186:1223	an unidentified phosphatidylglycolipid	1186:1223	The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylserine, an unidentified phosphatidylglycolipid, two unidentified phosphoaminolipids and an unidentified phosphoglycoaminolipid.
31951190	7	91	theme	major	1082:1086	arg1	lipids					1094:1099	The major polar lipids	1078:1099	The major polar lipids	1078:1099	The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylserine, an unidentified phosphatidylglycolipid, two unidentified phosphoaminolipids and an unidentified phosphoglycoaminolipid.
31951190	7	91	theme	major	1082:1086	arg1	phosphoglycoaminolipid					1282:1303	an unidentified phosphoglycoaminolipid	1266:1303	an unidentified phosphoglycoaminolipid	1266:1303	The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylserine, an unidentified phosphatidylglycolipid, two unidentified phosphoaminolipids and an unidentified phosphoglycoaminolipid.
31951190	7	91	theme	major	1082:1086	arg1	phosphatidylserine					1166:1183	phosphatidylserine	1166:1183	phosphatidylserine	1166:1183	The major polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylserine, an unidentified phosphatidylglycolipid, two unidentified phosphoaminolipids and an unidentified phosphoglycoaminolipid.
32749597	3	0	theme	DA	737:738	arg1	performance					722:732	impressive electrocatalytic oxidation performance	684:732	impressive electrocatalytic oxidation performance of DA and UA	684:745	The as-prepared modified electrode exhibited impressive electrocatalytic oxidation performance of DA and UA at an optimum working potential (0.172 V vs. Ag/AgCl for DA and 0.288 V vs. Ag/AgCl for UA) with linear detection range from 1.25 × 10-6 to 2.81 × 10-4 M and 0.75 × 10-6 to 1.66 × 10-4 M and the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA and UA, respectively.
32749597	3	1	theme	working	761:767	arg1	potential					769:777	an optimum working potential	750:777	an optimum working potential (0.172 V vs. Ag/AgCl for DA and 0.288 V vs. Ag/AgCl for UA)	750:837	The as-prepared modified electrode exhibited impressive electrocatalytic oxidation performance of DA and UA at an optimum working potential (0.172 V vs. Ag/AgCl for DA and 0.288 V vs. Ag/AgCl for UA) with linear detection range from 1.25 × 10-6 to 2.81 × 10-4 M and 0.75 × 10-6 to 1.66 × 10-4 M and the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA and UA, respectively.
32749597	3	2	theme	UA	744:745	arg1	performance					722:732	impressive electrocatalytic oxidation performance	684:732	impressive electrocatalytic oxidation performance of DA and UA	684:745	The as-prepared modified electrode exhibited impressive electrocatalytic oxidation performance of DA and UA at an optimum working potential (0.172 V vs. Ag/AgCl for DA and 0.288 V vs. Ag/AgCl for UA) with linear detection range from 1.25 × 10-6 to 2.81 × 10-4 M and 0.75 × 10-6 to 1.66 × 10-4 M and the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA and UA, respectively.
32749597	4	3	theme	peak-to-peak	1035:1046	arg1	separation					1048:1057	the peak-to-peak separation	1031:1057	the peak-to-peak separation	1031:1057	Additionally, the peak-to-peak separation signals in DPV are 116 mV.
32749597	6	4	from	UA	1257:1258	arg1	serums					1269:1274	human serums	1263:1274	human serums	1263:1274	The applicability of the method for real samples analysis was tested by determination of DA and UA in human serums.
32749597	5	5	theme	substances	1132:1141	arg1	influence					1090:1098	The influence	1086:1098	The influence of several possible co-existing substances	1086:1141	The influence of several possible co-existing substances was investigated.
32749597	3	6	theme	oxidation	712:720	arg1	performance					722:732	impressive electrocatalytic oxidation performance	684:732	impressive electrocatalytic oxidation performance of DA and UA	684:745	The as-prepared modified electrode exhibited impressive electrocatalytic oxidation performance of DA and UA at an optimum working potential (0.172 V vs. Ag/AgCl for DA and 0.288 V vs. Ag/AgCl for UA) with linear detection range from 1.25 × 10-6 to 2.81 × 10-4 M and 0.75 × 10-6 to 1.66 × 10-4 M and the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA and UA, respectively.
32749597	0	7	theme	acid	196:199	arg1	determination					161:173	the voltammetric simultaneous determination	131:173	the voltammetric simultaneous determination of dopamine and uric acid	131:199	Construction of an ultra-sensitive electrochemical sensor based on polyoxometalates decorated with CNTs and AuCo nanoparticles for the voltammetric simultaneous determination of dopamine and uric acid.
32749597	8	8	dep	abstract	1385:1392	arg1	Graphical					1375:1383	Graphical	1375:1383	Graphical	1375:1383	Graphical abstract.
32749597	5	9	theme	possible	1111:1118	arg1	substances					1132:1141	several possible co-existing substances	1103:1141	several possible co-existing substances	1103:1141	The influence of several possible co-existing substances was investigated.
32749597	3	10	theme	as-prepared	643:653	arg1	electrode					664:672	The as-prepared modified electrode	639:672	The as-prepared modified electrode	639:672	The as-prepared modified electrode exhibited impressive electrocatalytic oxidation performance of DA and UA at an optimum working potential (0.172 V vs. Ag/AgCl for DA and 0.288 V vs. Ag/AgCl for UA) with linear detection range from 1.25 × 10-6 to 2.81 × 10-4 M and 0.75 × 10-6 to 1.66 × 10-4 M and the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA and UA, respectively.
32749597	2	11	theme	electrical	574:583	arg1	conductivity					585:596	high electrical conductivity	569:596	high electrical conductivity	569:596	The combination of three active components endows the electrode with large specific surface area, high electrical conductivity, and excellent electrochemical activity.
32749597	6	12	theme	UA	1257:1258	arg1	determination					1233:1245	determination	1233:1245	determination of DA and UA in human serums	1233:1274	The applicability of the method for real samples analysis was tested by determination of DA and UA in human serums.
32749597	6	13	theme	real	1197:1200	arg1	analysis					1210:1217	real samples analysis	1197:1217	real samples analysis	1197:1217	The applicability of the method for real samples analysis was tested by determination of DA and UA in human serums.
32749597	2	14	theme	components	503:512	arg1	combination					475:485	The combination	471:485	The combination of three active components	471:512	The combination of three active components endows the electrode with large specific surface area, high electrical conductivity, and excellent electrochemical activity.
32749597	1	15	theme	stable	352:357	arg1	sensor					396:401	a structurally stable and highly sensitive electrochemical sensor	337:401	a structurally stable and highly sensitive electrochemical sensor for simultaneous determination of dopamine (DA) and uric acid (UA)	337:468	A composite modified electrode was prepared based on α-K7P2VW17O62·18H2O (P2W17V), CNTs and AuCo nanoparticles (AuCo NPs), and used as a structurally stable and highly sensitive electrochemical sensor for simultaneous determination of dopamine (DA) and uric acid (UA).
32749597	1	15	theme	stable	352:357	arg1	electrode					223:231	A composite modified electrode	202:231	A composite modified electrode	202:231	A composite modified electrode was prepared based on α-K7P2VW17O62·18H2O (P2W17V), CNTs and AuCo nanoparticles (AuCo NPs), and used as a structurally stable and highly sensitive electrochemical sensor for simultaneous determination of dopamine (DA) and uric acid (UA).
32749597	2	16	theme	high	569:572	arg1	conductivity					585:596	high electrical conductivity	569:596	high electrical conductivity	569:596	The combination of three active components endows the electrode with large specific surface area, high electrical conductivity, and excellent electrochemical activity.
32749597	2	17	theme	active	496:501	arg1	components					503:512	three active components	490:512	three active components	490:512	The combination of three active components endows the electrode with large specific surface area, high electrical conductivity, and excellent electrochemical activity.
32749597	6	18	theme	human	1263:1267	arg1	serums					1269:1274	human serums	1263:1274	human serums	1263:1274	The applicability of the method for real samples analysis was tested by determination of DA and UA in human serums.
32749597	1	19	theme	uric	455:458	arg1	UA					466:467	UA	466:467	UA	466:467	A composite modified electrode was prepared based on α-K7P2VW17O62·18H2O (P2W17V), CNTs and AuCo nanoparticles (AuCo NPs), and used as a structurally stable and highly sensitive electrochemical sensor for simultaneous determination of dopamine (DA) and uric acid (UA).
32749597	1	19	theme	uric	455:458	arg1	acid					460:463	uric acid	455:463	uric acid (UA)	455:468	A composite modified electrode was prepared based on α-K7P2VW17O62·18H2O (P2W17V), CNTs and AuCo nanoparticles (AuCo NPs), and used as a structurally stable and highly sensitive electrochemical sensor for simultaneous determination of dopamine (DA) and uric acid (UA).
32749597	6	20	theme	method	1186:1191	arg1	applicability					1165:1177	The applicability	1161:1177	The applicability of the method for real samples analysis	1161:1217	The applicability of the method for real samples analysis was tested by determination of DA and UA in human serums.
32749597	3	21	dep	0.15	961:964	arg1	S/N = 3					979:985	S/N = 3	979:985	S/N = 3	979:985	The as-prepared modified electrode exhibited impressive electrocatalytic oxidation performance of DA and UA at an optimum working potential (0.172 V vs. Ag/AgCl for DA and 0.288 V vs. Ag/AgCl for UA) with linear detection range from 1.25 × 10-6 to 2.81 × 10-4 M and 0.75 × 10-6 to 1.66 × 10-4 M and the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA and UA, respectively.
32749597	5	22	theme	several	1103:1109	arg1	substances					1132:1141	several possible co-existing substances	1103:1141	several possible co-existing substances	1103:1141	The influence of several possible co-existing substances was investigated.
32749597	2	23	theme	electrochemical	613:627	arg1	activity					629:636	excellent electrochemical activity	603:636	excellent electrochemical activity	603:636	The combination of three active components endows the electrode with large specific surface area, high electrical conductivity, and excellent electrochemical activity.
32749597	1	24	theme	acid	460:463	arg1	determination					420:432	simultaneous determination	407:432	simultaneous determination of dopamine (DA) and uric acid (UA)	407:468	A composite modified electrode was prepared based on α-K7P2VW17O62·18H2O (P2W17V), CNTs and AuCo nanoparticles (AuCo NPs), and used as a structurally stable and highly sensitive electrochemical sensor for simultaneous determination of dopamine (DA) and uric acid (UA).
32749597	3	25	from	2.81 × 10-4 M	887:899	arg1	range					861:865	linear detection range	844:865	linear detection range from 1.25 × 10-6 to 2.81 × 10-4 M and 0.75 × 10-6 to 1.66 × 10-4 M and the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA and UA, respectively	844:1014	The as-prepared modified electrode exhibited impressive electrocatalytic oxidation performance of DA and UA at an optimum working potential (0.172 V vs. Ag/AgCl for DA and 0.288 V vs. Ag/AgCl for UA) with linear detection range from 1.25 × 10-6 to 2.81 × 10-4 M and 0.75 × 10-6 to 1.66 × 10-4 M and the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA and UA, respectively.
32749597	1	26	theme	sensitive	370:378	arg1	sensor					396:401	a structurally stable and highly sensitive electrochemical sensor	337:401	a structurally stable and highly sensitive electrochemical sensor for simultaneous determination of dopamine (DA) and uric acid (UA)	337:468	A composite modified electrode was prepared based on α-K7P2VW17O62·18H2O (P2W17V), CNTs and AuCo nanoparticles (AuCo NPs), and used as a structurally stable and highly sensitive electrochemical sensor for simultaneous determination of dopamine (DA) and uric acid (UA).
32749597	1	26	theme	sensitive	370:378	arg1	electrode					223:231	A composite modified electrode	202:231	A composite modified electrode	202:231	A composite modified electrode was prepared based on α-K7P2VW17O62·18H2O (P2W17V), CNTs and AuCo nanoparticles (AuCo NPs), and used as a structurally stable and highly sensitive electrochemical sensor for simultaneous determination of dopamine (DA) and uric acid (UA).
32749597	2	27	theme	excellent	603:611	arg1	activity					629:636	excellent electrochemical activity	603:636	excellent electrochemical activity	603:636	The combination of three active components endows the electrode with large specific surface area, high electrical conductivity, and excellent electrochemical activity.
32749597	1	28	theme	A composite	202:212	arg1	sensor					396:401	a structurally stable and highly sensitive electrochemical sensor	337:401	a structurally stable and highly sensitive electrochemical sensor for simultaneous determination of dopamine (DA) and uric acid (UA)	337:468	A composite modified electrode was prepared based on α-K7P2VW17O62·18H2O (P2W17V), CNTs and AuCo nanoparticles (AuCo NPs), and used as a structurally stable and highly sensitive electrochemical sensor for simultaneous determination of dopamine (DA) and uric acid (UA).
32749597	1	28	theme	A composite	202:212	arg1	electrode					223:231	A composite modified electrode	202:231	A composite modified electrode	202:231	A composite modified electrode was prepared based on α-K7P2VW17O62·18H2O (P2W17V), CNTs and AuCo nanoparticles (AuCo NPs), and used as a structurally stable and highly sensitive electrochemical sensor for simultaneous determination of dopamine (DA) and uric acid (UA).
32749597	3	29	dep	potential	769:777	arg1	Ag/AgCl					792:798	Ag/AgCl	792:798	Ag/AgCl for DA and 0.288 V vs. Ag/AgCl for UA	792:836	The as-prepared modified electrode exhibited impressive electrocatalytic oxidation performance of DA and UA at an optimum working potential (0.172 V vs. Ag/AgCl for DA and 0.288 V vs. Ag/AgCl for UA) with linear detection range from 1.25 × 10-6 to 2.81 × 10-4 M and 0.75 × 10-6 to 1.66 × 10-4 M and the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA and UA, respectively.
32749597	3	29	dep	potential	769:777	arg1	0.172 V					780:786	0.172 V	780:786	0.172 V	780:786	The as-prepared modified electrode exhibited impressive electrocatalytic oxidation performance of DA and UA at an optimum working potential (0.172 V vs. Ag/AgCl for DA and 0.288 V vs. Ag/AgCl for UA) with linear detection range from 1.25 × 10-6 to 2.81 × 10-4 M and 0.75 × 10-6 to 1.66 × 10-4 M and the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA and UA, respectively.
32749597	1	30	theme	AuCo	294:297	arg1	nanoparticles					299:311	AuCo nanoparticles	294:311	AuCo nanoparticles (AuCo NPs)	294:322	A composite modified electrode was prepared based on α-K7P2VW17O62·18H2O (P2W17V), CNTs and AuCo nanoparticles (AuCo NPs), and used as a structurally stable and highly sensitive electrochemical sensor for simultaneous determination of dopamine (DA) and uric acid (UA).
32749597	1	30	theme	AuCo	294:297	arg1	NPs					319:321	AuCo NPs	314:321	AuCo NPs	314:321	A composite modified electrode was prepared based on α-K7P2VW17O62·18H2O (P2W17V), CNTs and AuCo nanoparticles (AuCo NPs), and used as a structurally stable and highly sensitive electrochemical sensor for simultaneous determination of dopamine (DA) and uric acid (UA).
32749597	1	30	theme	AuCo	294:297	arg1	α-K7P2VW17O62·18H2O					255:273	α-K7P2VW17O62·18H2O	255:273	α-K7P2VW17O62·18H2O (P2W17V)	255:282	A composite modified electrode was prepared based on α-K7P2VW17O62·18H2O (P2W17V), CNTs and AuCo nanoparticles (AuCo NPs), and used as a structurally stable and highly sensitive electrochemical sensor for simultaneous determination of dopamine (DA) and uric acid (UA).
32749597	3	31	theme	detection	942:950	arg1	limit					952:956	the detection limit	938:956	the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA	938:993	The as-prepared modified electrode exhibited impressive electrocatalytic oxidation performance of DA and UA at an optimum working potential (0.172 V vs. Ag/AgCl for DA and 0.288 V vs. Ag/AgCl for UA) with linear detection range from 1.25 × 10-6 to 2.81 × 10-4 M and 0.75 × 10-6 to 1.66 × 10-4 M and the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA and UA, respectively.
32749597	1	32	theme	modified	214:221	arg1	sensor					396:401	a structurally stable and highly sensitive electrochemical sensor	337:401	a structurally stable and highly sensitive electrochemical sensor for simultaneous determination of dopamine (DA) and uric acid (UA)	337:468	A composite modified electrode was prepared based on α-K7P2VW17O62·18H2O (P2W17V), CNTs and AuCo nanoparticles (AuCo NPs), and used as a structurally stable and highly sensitive electrochemical sensor for simultaneous determination of dopamine (DA) and uric acid (UA).
32749597	1	32	theme	modified	214:221	arg1	electrode					223:231	A composite modified electrode	202:231	A composite modified electrode	202:231	A composite modified electrode was prepared based on α-K7P2VW17O62·18H2O (P2W17V), CNTs and AuCo nanoparticles (AuCo NPs), and used as a structurally stable and highly sensitive electrochemical sensor for simultaneous determination of dopamine (DA) and uric acid (UA).
32749597	0	33	theme	ultra-sensitive	19:33	arg1	sensor					51:56	an ultra-sensitive electrochemical sensor	16:56	an ultra-sensitive electrochemical sensor based on polyoxometalates decorated with CNTs and AuCo	16:111	Construction of an ultra-sensitive electrochemical sensor based on polyoxometalates decorated with CNTs and AuCo nanoparticles for the voltammetric simultaneous determination of dopamine and uric acid.
32749597	0	34	theme	voltammetric	135:146	arg1	determination					161:173	the voltammetric simultaneous determination	131:173	the voltammetric simultaneous determination of dopamine and uric acid	131:199	Construction of an ultra-sensitive electrochemical sensor based on polyoxometalates decorated with CNTs and AuCo nanoparticles for the voltammetric simultaneous determination of dopamine and uric acid.
32749597	3	35	dep	2.81 × 10-4 M	887:899	arg1	to					884:885	to	884:885	to	884:885	The as-prepared modified electrode exhibited impressive electrocatalytic oxidation performance of DA and UA at an optimum working potential (0.172 V vs. Ag/AgCl for DA and 0.288 V vs. Ag/AgCl for UA) with linear detection range from 1.25 × 10-6 to 2.81 × 10-4 M and 0.75 × 10-6 to 1.66 × 10-4 M and the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA and UA, respectively.
32749597	3	35	dep	2.81 × 10-4 M	887:899	arg1	UA					999:1000	UA	999:1000	UA	999:1000	The as-prepared modified electrode exhibited impressive electrocatalytic oxidation performance of DA and UA at an optimum working potential (0.172 V vs. Ag/AgCl for DA and 0.288 V vs. Ag/AgCl for UA) with linear detection range from 1.25 × 10-6 to 2.81 × 10-4 M and 0.75 × 10-6 to 1.66 × 10-4 M and the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA and UA, respectively.
32749597	3	35	dep	2.81 × 10-4 M	887:899	arg1	limit					952:956	the detection limit	938:956	the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA	938:993	The as-prepared modified electrode exhibited impressive electrocatalytic oxidation performance of DA and UA at an optimum working potential (0.172 V vs. Ag/AgCl for DA and 0.288 V vs. Ag/AgCl for UA) with linear detection range from 1.25 × 10-6 to 2.81 × 10-4 M and 0.75 × 10-6 to 1.66 × 10-4 M and the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA and UA, respectively.
32749597	7	36	theme	great	1299:1303	arg1	promise					1305:1311	great promise	1299:1311	great promise for sensitive determination of DA and UA	1299:1352	This new sensor holds great promise for sensitive determination of DA and UA in real application.
32749597	3	37	theme	optimum	753:759	arg1	potential					769:777	an optimum working potential	750:777	an optimum working potential (0.172 V vs. Ag/AgCl for DA and 0.288 V vs. Ag/AgCl for UA)	750:837	The as-prepared modified electrode exhibited impressive electrocatalytic oxidation performance of DA and UA at an optimum working potential (0.172 V vs. Ag/AgCl for DA and 0.288 V vs. Ag/AgCl for UA) with linear detection range from 1.25 × 10-6 to 2.81 × 10-4 M and 0.75 × 10-6 to 1.66 × 10-4 M and the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA and UA, respectively.
32749597	1	38	theme	electrochemical	380:394	arg1	sensor					396:401	a structurally stable and highly sensitive electrochemical sensor	337:401	a structurally stable and highly sensitive electrochemical sensor for simultaneous determination of dopamine (DA) and uric acid (UA)	337:468	A composite modified electrode was prepared based on α-K7P2VW17O62·18H2O (P2W17V), CNTs and AuCo nanoparticles (AuCo NPs), and used as a structurally stable and highly sensitive electrochemical sensor for simultaneous determination of dopamine (DA) and uric acid (UA).
32749597	1	38	theme	electrochemical	380:394	arg1	electrode					223:231	A composite modified electrode	202:231	A composite modified electrode	202:231	A composite modified electrode was prepared based on α-K7P2VW17O62·18H2O (P2W17V), CNTs and AuCo nanoparticles (AuCo NPs), and used as a structurally stable and highly sensitive electrochemical sensor for simultaneous determination of dopamine (DA) and uric acid (UA).
32749597	0	39	theme	sensor	51:56	arg1	Construction					0:11	Construction	0:11	Construction of an ultra-sensitive electrochemical sensor based on polyoxometalates decorated with CNTs and AuCo	0:111	Construction of an ultra-sensitive electrochemical sensor based on polyoxometalates decorated with CNTs and AuCo nanoparticles for the voltammetric simultaneous determination of dopamine and uric acid.
32749597	1	40	theme	AuCo	314:317	arg1	nanoparticles					299:311	AuCo nanoparticles	294:311	AuCo nanoparticles (AuCo NPs)	294:322	A composite modified electrode was prepared based on α-K7P2VW17O62·18H2O (P2W17V), CNTs and AuCo nanoparticles (AuCo NPs), and used as a structurally stable and highly sensitive electrochemical sensor for simultaneous determination of dopamine (DA) and uric acid (UA).
32749597	1	40	theme	AuCo	314:317	arg1	NPs					319:321	AuCo NPs	314:321	AuCo NPs	314:321	A composite modified electrode was prepared based on α-K7P2VW17O62·18H2O (P2W17V), CNTs and AuCo nanoparticles (AuCo NPs), and used as a structurally stable and highly sensitive electrochemical sensor for simultaneous determination of dopamine (DA) and uric acid (UA).
32749597	3	41	theme	modified	655:662	arg1	electrode					664:672	The as-prepared modified electrode	639:672	The as-prepared modified electrode	639:672	The as-prepared modified electrode exhibited impressive electrocatalytic oxidation performance of DA and UA at an optimum working potential (0.172 V vs. Ag/AgCl for DA and 0.288 V vs. Ag/AgCl for UA) with linear detection range from 1.25 × 10-6 to 2.81 × 10-4 M and 0.75 × 10-6 to 1.66 × 10-4 M and the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA and UA, respectively.
32749597	6	42	theme	DA	1250:1251	arg1	determination					1233:1245	determination	1233:1245	determination of DA and UA in human serums	1233:1274	The applicability of the method for real samples analysis was tested by determination of DA and UA in human serums.
32749597	6	43	theme	samples	1202:1208	arg1	analysis					1210:1217	real samples analysis	1197:1217	real samples analysis	1197:1217	The applicability of the method for real samples analysis was tested by determination of DA and UA in human serums.
32749597	0	44	theme	electrochemical	35:49	arg1	sensor					51:56	an ultra-sensitive electrochemical sensor	16:56	an ultra-sensitive electrochemical sensor based on polyoxometalates decorated with CNTs and AuCo	16:111	Construction of an ultra-sensitive electrochemical sensor based on polyoxometalates decorated with CNTs and AuCo nanoparticles for the voltammetric simultaneous determination of dopamine and uric acid.
32749597	0	45	theme	simultaneous	148:159	arg1	determination					161:173	the voltammetric simultaneous determination	131:173	the voltammetric simultaneous determination of dopamine and uric acid	131:199	Construction of an ultra-sensitive electrochemical sensor based on polyoxometalates decorated with CNTs and AuCo nanoparticles for the voltammetric simultaneous determination of dopamine and uric acid.
32749597	0	46	theme	dopamine	178:185	arg1	determination					161:173	the voltammetric simultaneous determination	131:173	the voltammetric simultaneous determination of dopamine and uric acid	131:199	Construction of an ultra-sensitive electrochemical sensor based on polyoxometalates decorated with CNTs and AuCo nanoparticles for the voltammetric simultaneous determination of dopamine and uric acid.
32749597	1	47	theme	simultaneous	407:418	arg1	determination					420:432	simultaneous determination	407:432	simultaneous determination of dopamine (DA) and uric acid (UA)	407:468	A composite modified electrode was prepared based on α-K7P2VW17O62·18H2O (P2W17V), CNTs and AuCo nanoparticles (AuCo NPs), and used as a structurally stable and highly sensitive electrochemical sensor for simultaneous determination of dopamine (DA) and uric acid (UA).
32749597	7	48	theme	real	1357:1360	arg1	application					1362:1372	real application	1357:1372	real application	1357:1372	This new sensor holds great promise for sensitive determination of DA and UA in real application.
32749597	0	49	theme	uric	191:194	arg1	acid					196:199	uric acid	191:199	uric acid	191:199	Construction of an ultra-sensitive electrochemical sensor based on polyoxometalates decorated with CNTs and AuCo nanoparticles for the voltammetric simultaneous determination of dopamine and uric acid.
32749597	2	50	theme	large	540:544	arg1	area					563:566	large specific surface area	540:566	large specific surface area	540:566	The combination of three active components endows the electrode with large specific surface area, high electrical conductivity, and excellent electrochemical activity.
32749597	3	51	theme	impressive	684:693	arg1	performance					722:732	impressive electrocatalytic oxidation performance	684:732	impressive electrocatalytic oxidation performance of DA and UA	684:745	The as-prepared modified electrode exhibited impressive electrocatalytic oxidation performance of DA and UA at an optimum working potential (0.172 V vs. Ag/AgCl for DA and 0.288 V vs. Ag/AgCl for UA) with linear detection range from 1.25 × 10-6 to 2.81 × 10-4 M and 0.75 × 10-6 to 1.66 × 10-4 M and the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA and UA, respectively.
32749597	7	52	theme	new	1282:1284	arg1	sensor					1286:1291	This new sensor	1277:1291	This new sensor	1277:1291	This new sensor holds great promise for sensitive determination of DA and UA in real application.
32749597	3	53	theme	detection	851:859	arg1	range					861:865	linear detection range	844:865	linear detection range from 1.25 × 10-6 to 2.81 × 10-4 M and 0.75 × 10-6 to 1.66 × 10-4 M and the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA and UA, respectively	844:1014	The as-prepared modified electrode exhibited impressive electrocatalytic oxidation performance of DA and UA at an optimum working potential (0.172 V vs. Ag/AgCl for DA and 0.288 V vs. Ag/AgCl for UA) with linear detection range from 1.25 × 10-6 to 2.81 × 10-4 M and 0.75 × 10-6 to 1.66 × 10-4 M and the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA and UA, respectively.
32749597	1	54	used	used	329:332	arg2	electrode					223:231	A composite modified electrode	202:231	A composite modified electrode	202:231	A composite modified electrode was prepared based on α-K7P2VW17O62·18H2O (P2W17V), CNTs and AuCo nanoparticles (AuCo NPs), and used as a structurally stable and highly sensitive electrochemical sensor for simultaneous determination of dopamine (DA) and uric acid (UA).
32749597	1	54	used	used	329:332	arg2	sensor					396:401	a structurally stable and highly sensitive electrochemical sensor	337:401	a structurally stable and highly sensitive electrochemical sensor for simultaneous determination of dopamine (DA) and uric acid (UA)	337:468	A composite modified electrode was prepared based on α-K7P2VW17O62·18H2O (P2W17V), CNTs and AuCo nanoparticles (AuCo NPs), and used as a structurally stable and highly sensitive electrochemical sensor for simultaneous determination of dopamine (DA) and uric acid (UA).
32749597	2	55	theme	surface	555:561	arg1	area					563:566	large specific surface area	540:566	large specific surface area	540:566	The combination of three active components endows the electrode with large specific surface area, high electrical conductivity, and excellent electrochemical activity.
32749597	3	56	theme	electrocatalytic	695:710	arg1	performance					722:732	impressive electrocatalytic oxidation performance	684:732	impressive electrocatalytic oxidation performance of DA and UA	684:745	The as-prepared modified electrode exhibited impressive electrocatalytic oxidation performance of DA and UA at an optimum working potential (0.172 V vs. Ag/AgCl for DA and 0.288 V vs. Ag/AgCl for UA) with linear detection range from 1.25 × 10-6 to 2.81 × 10-4 M and 0.75 × 10-6 to 1.66 × 10-4 M and the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA and UA, respectively.
32749597	7	57	theme	UA	1351:1352	arg1	determination					1327:1339	sensitive determination	1317:1339	sensitive determination of DA and UA	1317:1352	This new sensor holds great promise for sensitive determination of DA and UA in real application.
32749597	3	58	dep	1.66 × 10-4 M	920:932	arg1	to					917:918	to	917:918	to	917:918	The as-prepared modified electrode exhibited impressive electrocatalytic oxidation performance of DA and UA at an optimum working potential (0.172 V vs. Ag/AgCl for DA and 0.288 V vs. Ag/AgCl for UA) with linear detection range from 1.25 × 10-6 to 2.81 × 10-4 M and 0.75 × 10-6 to 1.66 × 10-4 M and the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA and UA, respectively.
32749597	4	59	theme	DPV	1070:1072	arg1	are 116 mV					1074:1083	DPV are 116 mV	1070:1083	DPV are 116 mV	1070:1083	Additionally, the peak-to-peak separation signals in DPV are 116 mV.
32749597	2	60	theme	specific	546:553	arg1	area					563:566	large specific surface area	540:566	large specific surface area	540:566	The combination of three active components endows the electrode with large specific surface area, high electrical conductivity, and excellent electrochemical activity.
32749597	1	61	theme	dopamine	437:444	arg1	determination					420:432	simultaneous determination	407:432	simultaneous determination of dopamine (DA) and uric acid (UA)	407:468	A composite modified electrode was prepared based on α-K7P2VW17O62·18H2O (P2W17V), CNTs and AuCo nanoparticles (AuCo NPs), and used as a structurally stable and highly sensitive electrochemical sensor for simultaneous determination of dopamine (DA) and uric acid (UA).
32749597	6	62	from	determination	1233:1245	arg1	serums					1269:1274	human serums	1263:1274	human serums	1263:1274	The applicability of the method for real samples analysis was tested by determination of DA and UA in human serums.
32749597	7	63	theme	sensitive	1317:1325	arg1	determination					1327:1339	sensitive determination	1317:1339	sensitive determination of DA and UA	1317:1352	This new sensor holds great promise for sensitive determination of DA and UA in real application.
32749597	6	64	from	DA	1250:1251	arg1	serums					1269:1274	human serums	1263:1274	human serums	1263:1274	The applicability of the method for real samples analysis was tested by determination of DA and UA in human serums.
32749597	6	65	from	serums	1269:1274	arg1	determination					1233:1245	determination	1233:1245	determination of DA and UA in human serums	1233:1274	The applicability of the method for real samples analysis was tested by determination of DA and UA in human serums.
32749597	7	66	theme	DA	1344:1345	arg1	determination					1327:1339	sensitive determination	1317:1339	sensitive determination of DA and UA	1317:1352	This new sensor holds great promise for sensitive determination of DA and UA in real application.
32749597	3	67	theme	 for DA	987:993	arg1	UA					999:1000	UA	999:1000	UA	999:1000	The as-prepared modified electrode exhibited impressive electrocatalytic oxidation performance of DA and UA at an optimum working potential (0.172 V vs. Ag/AgCl for DA and 0.288 V vs. Ag/AgCl for UA) with linear detection range from 1.25 × 10-6 to 2.81 × 10-4 M and 0.75 × 10-6 to 1.66 × 10-4 M and the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA and UA, respectively.
32749597	3	67	theme	 for DA	987:993	arg1	limit					952:956	the detection limit	938:956	the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA	938:993	The as-prepared modified electrode exhibited impressive electrocatalytic oxidation performance of DA and UA at an optimum working potential (0.172 V vs. Ag/AgCl for DA and 0.288 V vs. Ag/AgCl for UA) with linear detection range from 1.25 × 10-6 to 2.81 × 10-4 M and 0.75 × 10-6 to 1.66 × 10-4 M and the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA and UA, respectively.
32749597	3	68	theme	linear	844:849	arg1	range					861:865	linear detection range	844:865	linear detection range from 1.25 × 10-6 to 2.81 × 10-4 M and 0.75 × 10-6 to 1.66 × 10-4 M and the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA and UA, respectively	844:1014	The as-prepared modified electrode exhibited impressive electrocatalytic oxidation performance of DA and UA at an optimum working potential (0.172 V vs. Ag/AgCl for DA and 0.288 V vs. Ag/AgCl for UA) with linear detection range from 1.25 × 10-6 to 2.81 × 10-4 M and 0.75 × 10-6 to 1.66 × 10-4 M and the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA and UA, respectively.
32749597	5	69	theme	co-existing	1120:1130	arg1	substances					1132:1141	several possible co-existing substances	1103:1141	several possible co-existing substances	1103:1141	The influence of several possible co-existing substances was investigated.
32749597	3	70	from	1.66 × 10-4 M	920:932	arg1	range					861:865	linear detection range	844:865	linear detection range from 1.25 × 10-6 to 2.81 × 10-4 M and 0.75 × 10-6 to 1.66 × 10-4 M and the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA and UA, respectively	844:1014	The as-prepared modified electrode exhibited impressive electrocatalytic oxidation performance of DA and UA at an optimum working potential (0.172 V vs. Ag/AgCl for DA and 0.288 V vs. Ag/AgCl for UA) with linear detection range from 1.25 × 10-6 to 2.81 × 10-4 M and 0.75 × 10-6 to 1.66 × 10-4 M and the detection limit of 0.15 and 0.25 μM (S/N = 3) for DA and UA, respectively.
34836184	4	0	theme	barrier	499:505	arg1	integrity					507:515	gut barrier integrity	495:515	gut barrier integrity	495:515	ALS supplementation markedly improved colitis symptoms, gut barrier integrity, and goblet loss in DSS-treated mice.
34836184	5	1	theme	kinases/NF-κB	670:682	arg1	pathway					694:700	the mitogen-activated protein kinases/NF-κB signaling pathway	640:700	the mitogen-activated protein kinases/NF-κB signaling pathway	640:700	In addition, ALS inhibited colonic inflammation through the inhibition/activation of the mitogen-activated protein kinases/NF-κB signaling pathway.
34836184	7	2	theme	farnesoid	1049:1057	arg1	receptor					1061:1068	the farnesoid X receptor	1045:1068	the farnesoid X receptor signaling	1045:1078	The bile-acid-targeted metabolomic analysis showed that ALS recovered the microbial bile acid metabolism in the gut, enabling the activation of the farnesoid X receptor signaling by these acids, thus maintaining the intestinal homeostasis.
34836184	5	3	theme	signaling	684:692	arg1	pathway					694:700	the mitogen-activated protein kinases/NF-κB signaling pathway	640:700	the mitogen-activated protein kinases/NF-κB signaling pathway	640:700	In addition, ALS inhibited colonic inflammation through the inhibition/activation of the mitogen-activated protein kinases/NF-κB signaling pathway.
34836184	9	4	theme	nutritional	1328:1338	arg1	intervention					1340:1351	the nutritional intervention	1324:1351	the nutritional intervention to prevent colitis	1324:1370	Overall, our findings suggest that ALS may represent a novel approach in the nutritional intervention to prevent colitis.
34836184	7	5	theme	X	1059:1059	arg1	receptor					1061:1068	the farnesoid X receptor	1045:1068	the farnesoid X receptor signaling	1045:1078	The bile-acid-targeted metabolomic analysis showed that ALS recovered the microbial bile acid metabolism in the gut, enabling the activation of the farnesoid X receptor signaling by these acids, thus maintaining the intestinal homeostasis.
34836184	0	6	theme	Microbiota	106:115	arg1	Modulation					88:97	Modulation	88:97	Modulation of Gut Microbiota and Microbiota-Related Bile Acids	88:149	Armillaria luteo-virens Sacc Ameliorates Dextran Sulfate Sodium Induced Colitis through Modulation of Gut Microbiota and Microbiota-Related Bile Acids.
34836184	5	7	theme	pathway	694:700	arg1	inhibition/activation					615:635	the inhibition/activation	611:635	the inhibition/activation of the mitogen-activated protein kinases/NF-κB signaling pathway	611:700	In addition, ALS inhibited colonic inflammation through the inhibition/activation of the mitogen-activated protein kinases/NF-κB signaling pathway.
34836184	0	8	theme	Gut	102:104	arg1	Microbiota					106:115	Gut Microbiota	102:115	Gut Microbiota	102:115	Armillaria luteo-virens Sacc Ameliorates Dextran Sulfate Sodium Induced Colitis through Modulation of Gut Microbiota and Microbiota-Related Bile Acids.
34836184	6	9	theme	16S	707:709	arg1	rRNA					711:714	The 16S rRNA	703:714	The 16S rRNA gene-based microbiota analysis	703:745	The 16S rRNA gene-based microbiota analysis revealed that ALS altered the gut microbiota composition, decreasing the richness of Enterobacteriaceae and increasing the abundance of Lactobacillaceae.
34836184	7	10	theme	metabolomic	924:934	arg1	analysis					936:943	The bile-acid-targeted metabolomic analysis	901:943	The bile-acid-targeted metabolomic analysis	901:943	The bile-acid-targeted metabolomic analysis showed that ALS recovered the microbial bile acid metabolism in the gut, enabling the activation of the farnesoid X receptor signaling by these acids, thus maintaining the intestinal homeostasis.
34836184	0	11	theme	Microbiota-Related	121:138	arg1	Acids					145:149	Microbiota-Related Bile Acids	121:149	Microbiota-Related Bile Acids	121:149	Armillaria luteo-virens Sacc Ameliorates Dextran Sulfate Sodium Induced Colitis through Modulation of Gut Microbiota and Microbiota-Related Bile Acids.
34836184	4	12	theme	colitis	477:483	arg1	symptoms					485:492	colitis symptoms	477:492	colitis symptoms	477:492	ALS supplementation markedly improved colitis symptoms, gut barrier integrity, and goblet loss in DSS-treated mice.
34836184	2	13	theme	intestinal	280:289	arg1	functions					291:299	intestinal functions	280:299	intestinal functions	280:299	However, its protective effect on intestinal functions and the underlying mechanism is still unknown.
34836184	4	14	theme	gut	495:497	arg1	integrity					507:515	gut barrier integrity	495:515	gut barrier integrity	495:515	ALS supplementation markedly improved colitis symptoms, gut barrier integrity, and goblet loss in DSS-treated mice.
34836184	6	15	theme	Enterobacteriaceae	832:849	arg1	richness					820:827	the richness	816:827	the richness of Enterobacteriaceae	816:849	The 16S rRNA gene-based microbiota analysis revealed that ALS altered the gut microbiota composition, decreasing the richness of Enterobacteriaceae and increasing the abundance of Lactobacillaceae.
34836184	7	16	theme	bile-acid-targeted	905:922	arg1	analysis					936:943	The bile-acid-targeted metabolomic analysis	901:943	The bile-acid-targeted metabolomic analysis	901:943	The bile-acid-targeted metabolomic analysis showed that ALS recovered the microbial bile acid metabolism in the gut, enabling the activation of the farnesoid X receptor signaling by these acids, thus maintaining the intestinal homeostasis.
34836184	0	17	theme	luteo-virens	11:22	arg1	Sacc					24:27	Armillaria luteo-virens Sacc	0:27	Armillaria luteo-virens Sacc	0:27	Armillaria luteo-virens Sacc Ameliorates Dextran Sulfate Sodium Induced Colitis through Modulation of Gut Microbiota and Microbiota-Related Bile Acids.
34836184	5	18	theme	mitogen-activated	644:660	arg1	pathway					694:700	the mitogen-activated protein kinases/NF-κB signaling pathway	640:700	the mitogen-activated protein kinases/NF-κB signaling pathway	640:700	In addition, ALS inhibited colonic inflammation through the inhibition/activation of the mitogen-activated protein kinases/NF-κB signaling pathway.
34836184	6	19	theme	microbiota	727:736	arg1	analysis					738:745	The 16S rRNA gene-based microbiota analysis	703:745	The 16S rRNA gene-based microbiota analysis	703:745	The 16S rRNA gene-based microbiota analysis revealed that ALS altered the gut microbiota composition, decreasing the richness of Enterobacteriaceae and increasing the abundance of Lactobacillaceae.
34836184	3	20	theme	colitis	423:429	arg1	improvement					371:381	the improvement	367:381	the improvement of dextran sulfate sodium (DSS)-induced colitis by ALS	367:436	This work explored the improvement of dextran sulfate sodium (DSS)-induced colitis by ALS.
34836184	0	21	theme	Armillaria	0:9	arg1	Sacc					24:27	Armillaria luteo-virens Sacc	0:27	Armillaria luteo-virens Sacc	0:27	Armillaria luteo-virens Sacc Ameliorates Dextran Sulfate Sodium Induced Colitis through Modulation of Gut Microbiota and Microbiota-Related Bile Acids.
34836184	5	22	theme	protein	662:668	arg1	pathway					694:700	the mitogen-activated protein kinases/NF-κB signaling pathway	640:700	the mitogen-activated protein kinases/NF-κB signaling pathway	640:700	In addition, ALS inhibited colonic inflammation through the inhibition/activation of the mitogen-activated protein kinases/NF-κB signaling pathway.
34836184	6	23	theme	gene-based	716:725	arg1	analysis					738:745	The 16S rRNA gene-based microbiota analysis	703:745	The 16S rRNA gene-based microbiota analysis	703:745	The 16S rRNA gene-based microbiota analysis revealed that ALS altered the gut microbiota composition, decreasing the richness of Enterobacteriaceae and increasing the abundance of Lactobacillaceae.
34836184	1	24	theme	rare	192:195	arg1	Sacc					176:179	Armillaria luteo-virens Sacc	152:179	Armillaria luteo-virens Sacc (ALS)	152:185	Armillaria luteo-virens Sacc (ALS) is a rare wild Chinese medicinal and edible basidiomycete.
34836184	1	24	theme	rare	192:195	arg1	medicinal					210:218	a rare wild Chinese medicinal	190:218	a rare wild Chinese medicinal	190:218	Armillaria luteo-virens Sacc (ALS) is a rare wild Chinese medicinal and edible basidiomycete.
34836184	0	25	theme	Acids	145:149	arg1	Modulation					88:97	Modulation	88:97	Modulation of Gut Microbiota and Microbiota-Related Bile Acids	88:149	Armillaria luteo-virens Sacc Ameliorates Dextran Sulfate Sodium Induced Colitis through Modulation of Gut Microbiota and Microbiota-Related Bile Acids.
34836184	5	26	theme	colonic	582:588	arg1	inflammation					590:601	colonic inflammation	582:601	colonic inflammation	582:601	In addition, ALS inhibited colonic inflammation through the inhibition/activation of the mitogen-activated protein kinases/NF-κB signaling pathway.
34836184	2	27	theme	underlying	309:318	arg1	mechanism					320:328	the underlying mechanism	305:328	the underlying mechanism	305:328	However, its protective effect on intestinal functions and the underlying mechanism is still unknown.
34836184	6	28	theme	rRNA	711:714	arg1	analysis					738:745	The 16S rRNA gene-based microbiota analysis	703:745	The 16S rRNA gene-based microbiota analysis	703:745	The 16S rRNA gene-based microbiota analysis revealed that ALS altered the gut microbiota composition, decreasing the richness of Enterobacteriaceae and increasing the abundance of Lactobacillaceae.
34836184	1	29	theme	wild	197:200	arg1	Sacc					176:179	Armillaria luteo-virens Sacc	152:179	Armillaria luteo-virens Sacc (ALS)	152:185	Armillaria luteo-virens Sacc (ALS) is a rare wild Chinese medicinal and edible basidiomycete.
34836184	1	29	theme	wild	197:200	arg1	medicinal					210:218	a rare wild Chinese medicinal	190:218	a rare wild Chinese medicinal	190:218	Armillaria luteo-virens Sacc (ALS) is a rare wild Chinese medicinal and edible basidiomycete.
34836184	0	30	theme	Bile	140:143	arg1	Acids					145:149	Microbiota-Related Bile Acids	121:149	Microbiota-Related Bile Acids	121:149	Armillaria luteo-virens Sacc Ameliorates Dextran Sulfate Sodium Induced Colitis through Modulation of Gut Microbiota and Microbiota-Related Bile Acids.
34836184	8	31	from	colitis	1242:1248	arg1	efficacy					1202:1209	the efficacy	1198:1209	the efficacy of ALS-induced protection from colitis	1198:1248	Importantly, broad-spectrum antibiotic treatment reduced the efficacy of ALS-induced protection from colitis.
34836184	8	31	from	colitis	1242:1248	arg1	protection					1226:1235	ALS-induced protection	1214:1235	ALS-induced protection from colitis	1214:1248	Importantly, broad-spectrum antibiotic treatment reduced the efficacy of ALS-induced protection from colitis.
34836184	7	32	from	metabolism	995:1004	arg1	gut					1013:1015	the gut	1009:1015	the gut	1009:1015	The bile-acid-targeted metabolomic analysis showed that ALS recovered the microbial bile acid metabolism in the gut, enabling the activation of the farnesoid X receptor signaling by these acids, thus maintaining the intestinal homeostasis.
34836184	2	33	from	effect	270:275	arg1	functions					291:299	intestinal functions	280:299	intestinal functions	280:299	However, its protective effect on intestinal functions and the underlying mechanism is still unknown.
34836184	2	33	from	effect	270:275	arg1	mechanism					320:328	the underlying mechanism	305:328	the underlying mechanism	305:328	However, its protective effect on intestinal functions and the underlying mechanism is still unknown.
34836184	1	34	theme	Chinese	202:208	arg1	Sacc					176:179	Armillaria luteo-virens Sacc	152:179	Armillaria luteo-virens Sacc (ALS)	152:185	Armillaria luteo-virens Sacc (ALS) is a rare wild Chinese medicinal and edible basidiomycete.
34836184	1	34	theme	Chinese	202:208	arg1	medicinal					210:218	a rare wild Chinese medicinal	190:218	a rare wild Chinese medicinal	190:218	Armillaria luteo-virens Sacc (ALS) is a rare wild Chinese medicinal and edible basidiomycete.
34836184	0	35	theme	Sulfate	49:55	arg1	Colitis					72:78	Dextran Sulfate Sodium Induced Colitis	41:78	Dextran Sulfate Sodium Induced Colitis	41:78	Armillaria luteo-virens Sacc Ameliorates Dextran Sulfate Sodium Induced Colitis through Modulation of Gut Microbiota and Microbiota-Related Bile Acids.
34836184	7	36	theme	intestinal	1117:1126	arg1	homeostasis					1128:1138	the intestinal homeostasis	1113:1138	the intestinal homeostasis	1113:1138	The bile-acid-targeted metabolomic analysis showed that ALS recovered the microbial bile acid metabolism in the gut, enabling the activation of the farnesoid X receptor signaling by these acids, thus maintaining the intestinal homeostasis.
34836184	4	37	theme	ALS	439:441	arg1	supplementation					443:457	ALS supplementation	439:457	ALS supplementation	439:457	ALS supplementation markedly improved colitis symptoms, gut barrier integrity, and goblet loss in DSS-treated mice.
34836184	0	38	theme	Dextran	41:47	arg1	Sulfate					49:55	Dextran Sulfate	41:55	Dextran Sulfate Sodium Induced Colitis	41:78	Armillaria luteo-virens Sacc Ameliorates Dextran Sulfate Sodium Induced Colitis through Modulation of Gut Microbiota and Microbiota-Related Bile Acids.
34836184	3	39	theme	-induced	414:421	arg1	colitis					423:429	dextran sulfate sodium (DSS)-induced colitis	386:429	dextran sulfate sodium (DSS)-induced colitis	386:429	This work explored the improvement of dextran sulfate sodium (DSS)-induced colitis by ALS.
34836184	8	40	from	efficacy	1202:1209	arg1	colitis					1242:1248	colitis	1242:1248	colitis	1242:1248	Importantly, broad-spectrum antibiotic treatment reduced the efficacy of ALS-induced protection from colitis.
34836184	0	41	theme	Induced	64:70	arg1	Colitis					72:78	Dextran Sulfate Sodium Induced Colitis	41:78	Dextran Sulfate Sodium Induced Colitis	41:78	Armillaria luteo-virens Sacc Ameliorates Dextran Sulfate Sodium Induced Colitis through Modulation of Gut Microbiota and Microbiota-Related Bile Acids.
34836184	4	42	theme	goblet	522:527	arg1	loss					529:532	goblet loss	522:532	goblet loss	522:532	ALS supplementation markedly improved colitis symptoms, gut barrier integrity, and goblet loss in DSS-treated mice.
34836184	8	43	theme	ALS-induced	1214:1224	arg1	protection					1226:1235	ALS-induced protection	1214:1235	ALS-induced protection from colitis	1214:1248	Importantly, broad-spectrum antibiotic treatment reduced the efficacy of ALS-induced protection from colitis.
34836184	0	44	theme	Sodium	57:62	arg1	Colitis					72:78	Dextran Sulfate Sodium Induced Colitis	41:78	Dextran Sulfate Sodium Induced Colitis	41:78	Armillaria luteo-virens Sacc Ameliorates Dextran Sulfate Sodium Induced Colitis through Modulation of Gut Microbiota and Microbiota-Related Bile Acids.
34836184	4	45	theme	DSS-treated	537:547	arg1	mice					549:552	DSS-treated mice	537:552	DSS-treated mice	537:552	ALS supplementation markedly improved colitis symptoms, gut barrier integrity, and goblet loss in DSS-treated mice.
34836184	8	46	theme	broad-spectrum	1154:1167	arg1	treatment					1180:1188	broad-spectrum antibiotic treatment	1154:1188	broad-spectrum antibiotic treatment	1154:1188	Importantly, broad-spectrum antibiotic treatment reduced the efficacy of ALS-induced protection from colitis.
34836184	7	47	theme	signaling	1070:1078	arg1	activation					1031:1040	the activation	1027:1040	the activation of the farnesoid X receptor signaling by these acids	1027:1093	The bile-acid-targeted metabolomic analysis showed that ALS recovered the microbial bile acid metabolism in the gut, enabling the activation of the farnesoid X receptor signaling by these acids, thus maintaining the intestinal homeostasis.
34836184	6	48	dep	altered	765:771	arg1	increasing					855:864	increasing	855:864	increasing the abundance of Lactobacillaceae	855:898	The 16S rRNA gene-based microbiota analysis revealed that ALS altered the gut microbiota composition, decreasing the richness of Enterobacteriaceae and increasing the abundance of Lactobacillaceae.
34836184	6	48	dep	altered	765:771	arg1	decreasing					805:814	decreasing	805:814	decreasing the richness of Enterobacteriaceae	805:849	The 16S rRNA gene-based microbiota analysis revealed that ALS altered the gut microbiota composition, decreasing the richness of Enterobacteriaceae and increasing the abundance of Lactobacillaceae.
34836184	9	49	theme	novel	1306:1310	arg1	approach					1312:1319	a novel approach	1304:1319	a novel approach	1304:1319	Overall, our findings suggest that ALS may represent a novel approach in the nutritional intervention to prevent colitis.
34836184	6	50	theme	microbiota	781:790	arg1	composition					792:802	the gut microbiota composition	773:802	the gut microbiota composition	773:802	The 16S rRNA gene-based microbiota analysis revealed that ALS altered the gut microbiota composition, decreasing the richness of Enterobacteriaceae and increasing the abundance of Lactobacillaceae.
34836184	1	51	theme	edible	224:229	arg1	basidiomycete					231:243	edible basidiomycete	224:243	edible basidiomycete	224:243	Armillaria luteo-virens Sacc (ALS) is a rare wild Chinese medicinal and edible basidiomycete.
34836184	6	52	theme	Lactobacillaceae	883:898	arg1	abundance					870:878	the abundance	866:878	the abundance of Lactobacillaceae	866:898	The 16S rRNA gene-based microbiota analysis revealed that ALS altered the gut microbiota composition, decreasing the richness of Enterobacteriaceae and increasing the abundance of Lactobacillaceae.
34836184	2	53	theme	protective	259:268	arg1	effect					270:275	its protective effect	255:275	its protective effect on intestinal functions and the underlying mechanism	255:328	However, its protective effect on intestinal functions and the underlying mechanism is still unknown.
34836184	6	54	theme	gut	777:779	arg1	composition					792:802	the gut microbiota composition	773:802	the gut microbiota composition	773:802	The 16S rRNA gene-based microbiota analysis revealed that ALS altered the gut microbiota composition, decreasing the richness of Enterobacteriaceae and increasing the abundance of Lactobacillaceae.
34836184	1	55	theme	Armillaria	152:161	arg1	Sacc					176:179	Armillaria luteo-virens Sacc	152:179	Armillaria luteo-virens Sacc (ALS)	152:185	Armillaria luteo-virens Sacc (ALS) is a rare wild Chinese medicinal and edible basidiomycete.
34836184	1	55	theme	Armillaria	152:161	arg1	medicinal					210:218	a rare wild Chinese medicinal	190:218	a rare wild Chinese medicinal	190:218	Armillaria luteo-virens Sacc (ALS) is a rare wild Chinese medicinal and edible basidiomycete.
34836184	1	55	theme	Armillaria	152:161	arg1	ALS					182:184	ALS	182:184	ALS	182:184	Armillaria luteo-virens Sacc (ALS) is a rare wild Chinese medicinal and edible basidiomycete.
34836184	7	56	theme	microbial	975:983	arg1	metabolism					995:1004	the microbial bile acid metabolism	971:1004	the microbial bile acid metabolism in the gut	971:1015	The bile-acid-targeted metabolomic analysis showed that ALS recovered the microbial bile acid metabolism in the gut, enabling the activation of the farnesoid X receptor signaling by these acids, thus maintaining the intestinal homeostasis.
34836184	8	57	theme	protection	1226:1235	arg1	efficacy					1202:1209	the efficacy	1198:1209	the efficacy of ALS-induced protection from colitis	1198:1248	Importantly, broad-spectrum antibiotic treatment reduced the efficacy of ALS-induced protection from colitis.
34836184	7	58	theme	receptor	1061:1068	arg1	signaling					1070:1078	the farnesoid X receptor signaling	1045:1078	the farnesoid X receptor signaling	1045:1078	The bile-acid-targeted metabolomic analysis showed that ALS recovered the microbial bile acid metabolism in the gut, enabling the activation of the farnesoid X receptor signaling by these acids, thus maintaining the intestinal homeostasis.
34836184	1	59	theme	luteo-virens	163:174	arg1	Sacc					176:179	Armillaria luteo-virens Sacc	152:179	Armillaria luteo-virens Sacc (ALS)	152:185	Armillaria luteo-virens Sacc (ALS) is a rare wild Chinese medicinal and edible basidiomycete.
34836184	1	59	theme	luteo-virens	163:174	arg1	medicinal					210:218	a rare wild Chinese medicinal	190:218	a rare wild Chinese medicinal	190:218	Armillaria luteo-virens Sacc (ALS) is a rare wild Chinese medicinal and edible basidiomycete.
34836184	1	59	theme	luteo-virens	163:174	arg1	ALS					182:184	ALS	182:184	ALS	182:184	Armillaria luteo-virens Sacc (ALS) is a rare wild Chinese medicinal and edible basidiomycete.
34836184	7	60	theme	bile	985:988	arg1	metabolism					995:1004	the microbial bile acid metabolism	971:1004	the microbial bile acid metabolism in the gut	971:1015	The bile-acid-targeted metabolomic analysis showed that ALS recovered the microbial bile acid metabolism in the gut, enabling the activation of the farnesoid X receptor signaling by these acids, thus maintaining the intestinal homeostasis.
34836184	7	61	theme	acid	990:993	arg1	metabolism					995:1004	the microbial bile acid metabolism	971:1004	the microbial bile acid metabolism in the gut	971:1015	The bile-acid-targeted metabolomic analysis showed that ALS recovered the microbial bile acid metabolism in the gut, enabling the activation of the farnesoid X receptor signaling by these acids, thus maintaining the intestinal homeostasis.
34836184	8	62	theme	antibiotic	1169:1178	arg1	treatment					1180:1188	broad-spectrum antibiotic treatment	1154:1188	broad-spectrum antibiotic treatment	1154:1188	Importantly, broad-spectrum antibiotic treatment reduced the efficacy of ALS-induced protection from colitis.
31967956	9	0	theme	similarity	930:939	arg1	levels					911:916	the highest levels	899:916	the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %)	899:1143	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	12	1	theme	strain	1410:1415	arg1	py1325T					1417:1423	strain py1325T	1410:1423	strain py1325T	1410:1423	According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain py1325T clearly represents a novel species of the genus Paenibacillus, for which the name Paenibacillus paridis sp.
31967956	14	2	theme	2015220T=LMG	1589:1600	arg1	py1325T					1570:1576	py1325T	1570:1576	py1325T (=CCTCC AB 2015220T=LMG 29068T)	1570:1608	The type strain is py1325T (=CCTCC AB 2015220T=LMG 29068T).
31967956	14	2	theme	2015220T=LMG	1589:1600	arg1	29068T					1602:1607	=CCTCC AB 2015220T=LMG 29068T	1579:1607	=CCTCC AB 2015220T=LMG 29068T	1579:1607	The type strain is py1325T (=CCTCC AB 2015220T=LMG 29068T).
31967956	3	3	attach	isolated	232:239	arg2	strain					200:205	A Gram-reaction-positive, endospore-forming and rod-shaped bacterial strain	131:205	A Gram-reaction-positive, endospore-forming and rod-shaped bacterial strain	131:205	A Gram-reaction-positive, endospore-forming and rod-shaped bacterial strain, designated py1325T, was isolated from the root of Paris polyphylla Smith var.
31967956	3	3	attach	isolated	232:239	arg1	root					250:253	the root	246:253	the root of Paris polyphylla Smith var	246:283	A Gram-reaction-positive, endospore-forming and rod-shaped bacterial strain, designated py1325T, was isolated from the root of Paris polyphylla Smith var.
31967956	9	4	theme	gene	867:870	arg1	analysis					881:888	16S rRNA gene sequence analysis	858:888	16S rRNA gene sequence analysis	858:888	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	7	5	theme	major	597:601	arg1	 0					640:641	 0	640:641	 0	640:641	The major cellular fatty acid was anteiso-C15 : 0.
31967956	7	5	theme	major	597:601	arg1	acid					618:621	The major cellular fatty acid	593:621	The major cellular fatty acid	593:621	The major cellular fatty acid was anteiso-C15 : 0.
31967956	11	6	theme	genomic	1312:1318	arg1	DNA					1320:1322	the genomic DNA	1308:1322	the genomic DNA	1308:1322	The G+C content of the genomic DNA was 47.7 mol%.
31967956	5	7	theme	0-1 	421:424	arg1	w/v					433:435	w/v	433:435	w/v	433:435	It grew optimally with 0-1 % NaCl (w/v), at pH 7 and at 30 °C.
31967956	5	7	theme	0-1 	421:424	arg1	 NaCl					426:430	0-1 % NaCl	421:430	0-1 % NaCl (w/v)	421:436	It grew optimally with 0-1 % NaCl (w/v), at pH 7 and at 30 °C.
31967956	7	8	theme	cellular	603:610	arg1	 0					640:641	 0	640:641	 0	640:641	The major cellular fatty acid was anteiso-C15 : 0.
31967956	7	8	theme	cellular	603:610	arg1	acid					618:621	The major cellular fatty acid	593:621	The major cellular fatty acid	593:621	The major cellular fatty acid was anteiso-C15 : 0.
31967956	3	9	theme	rod-shaped	179:188	arg1	strain					200:205	A Gram-reaction-positive, endospore-forming and rod-shaped bacterial strain	131:205	A Gram-reaction-positive, endospore-forming and rod-shaped bacterial strain	131:205	A Gram-reaction-positive, endospore-forming and rod-shaped bacterial strain, designated py1325T, was isolated from the root of Paris polyphylla Smith var.
31967956	1	10	theme	bacterial	46:54	arg1	species					56:62	an endophytic bacterial species	32:62	an endophytic bacterial species isolated from the root of Paris polyphylla Smith var	32:115	nov., an endophytic bacterial species isolated from the root of Paris polyphylla Smith var.
31967956	6	11	theme	meso-diaminopimelic	567:585	arg1	acid					527:530	the diagnostic diamino acid	504:530	the diagnostic diamino acid in the cell-wall peptidoglycan	504:561	The major respiratory quinone was MK-7 and the diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
31967956	6	11	theme	meso-diaminopimelic	567:585	arg1	acid					587:590	meso-diaminopimelic acid	567:590	meso-diaminopimelic acid	567:590	The major respiratory quinone was MK-7 and the diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
31967956	10	12	theme	hybridization	1166:1178	arg1	values					1212:1217	The digital DNA-DNA hybridization and average nucleotide identity values	1146:1217	The digital DNA-DNA hybridization and average nucleotide identity values between py1325T and these species	1146:1251	The digital DNA-DNA hybridization and average nucleotide identity values between py1325T and these species ranged 20.6-53.3 % and 79.9-93.6 %.
31967956	6	13	theme	respiratory	471:481	arg1	MK-7					495:498	MK-7	495:498	MK-7	495:498	The major respiratory quinone was MK-7 and the diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
31967956	6	13	theme	respiratory	471:481	arg1	quinone					483:489	The major respiratory quinone	461:489	The major respiratory quinone	461:489	The major respiratory quinone was MK-7 and the diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
31967956	12	14	theme	chemotaxonomic	1385:1398	arg1	evidence					1400:1407	the phylogenetic, phenotypic and chemotaxonomic evidence	1352:1407	the phylogenetic, phenotypic and chemotaxonomic evidence	1352:1407	According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain py1325T clearly represents a novel species of the genus Paenibacillus, for which the name Paenibacillus paridis sp.
31967956	9	15	theme	Paenibacillus	1050:1062	arg1	19417T					1078:1083	Paenibacillus castaneae DSM 19417T	1050:1083	Paenibacillus castaneae DSM 19417T (97.5 %)	1050:1092	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	9	15	theme	Paenibacillus	1050:1062	arg1	%					1091:1091	97.5 %	1086:1091	97.5 %	1086:1091	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	10	16	theme	DNA-DNA	1158:1164	arg1	hybridization					1166:1178	digital DNA-DNA hybridization	1150:1178	digital DNA-DNA hybridization	1150:1178	The digital DNA-DNA hybridization and average nucleotide identity values between py1325T and these species ranged 20.6-53.3 % and 79.9-93.6 %.
31967956	6	17	theme	major	465:469	arg1	MK-7					495:498	MK-7	495:498	MK-7	495:498	The major respiratory quinone was MK-7 and the diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
31967956	6	17	theme	major	465:469	arg1	quinone					483:489	The major respiratory quinone	461:489	The major respiratory quinone	461:489	The major respiratory quinone was MK-7 and the diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
31967956	9	18	theme	16S	858:860	arg1	analysis					881:888	16S rRNA gene sequence analysis	858:888	16S rRNA gene sequence analysis	858:888	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	10	19	theme	 and	1271:1274	arg1	%					1286:1286	20.6-53.3 % and 79.9-93.6 %	1260:1286	20.6-53.3 % and 79.9-93.6 %	1260:1286	The digital DNA-DNA hybridization and average nucleotide identity values between py1325T and these species ranged 20.6-53.3 % and 79.9-93.6 %.
31967956	3	20	theme	endospore-forming	157:173	arg1	strain					200:205	A Gram-reaction-positive, endospore-forming and rod-shaped bacterial strain	131:205	A Gram-reaction-positive, endospore-forming and rod-shaped bacterial strain	131:205	A Gram-reaction-positive, endospore-forming and rod-shaped bacterial strain, designated py1325T, was isolated from the root of Paris polyphylla Smith var.
31967956	12	21	theme	phenotypic	1370:1379	arg1	evidence					1400:1407	the phylogenetic, phenotypic and chemotaxonomic evidence	1352:1407	the phylogenetic, phenotypic and chemotaxonomic evidence	1352:1407	According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain py1325T clearly represents a novel species of the genus Paenibacillus, for which the name Paenibacillus paridis sp.
31967956	9	22	theme	castaneae	1064:1072	arg1	19417T					1078:1083	Paenibacillus castaneae DSM 19417T	1050:1083	Paenibacillus castaneae DSM 19417T (97.5 %)	1050:1092	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	9	22	theme	castaneae	1064:1072	arg1	%					1091:1091	97.5 %	1086:1091	97.5 %	1086:1091	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	11	23	theme	DNA	1320:1322	arg1	%					1336:1336	47.7 mol%	1328:1336	47.7 mol%	1328:1336	The G+C content of the genomic DNA was 47.7 mol%.
31967956	11	23	theme	DNA	1320:1322	arg1	content					1297:1303	The G+C content	1289:1303	The G+C content of the genomic DNA	1289:1322	The G+C content of the genomic DNA was 47.7 mol%.
31967956	9	24	theme	luteus	971:976	arg1	R-3T					978:981	Paenibacillus luteus R-3T	957:981	Paenibacillus luteus R-3T (99.0 %)	957:990	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	9	24	theme	luteus	971:976	arg1	%					989:989	99.0 %	984:989	99.0 %	984:989	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	8	25	theme	unidentified	787:798	arg1	aminophospholipids					763:780	two unidentified aminophospholipids	746:780	two unidentified aminophospholipids	746:780	The polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phospholipids and two unidentified lipids.
31967956	8	25	theme	unidentified	787:798	arg1	phospholipids					800:812	two unidentified phospholipids	783:812	two unidentified phospholipids	783:812	The polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phospholipids and two unidentified lipids.
31967956	12	26	theme	Paenibacillus	1507:1519	arg1	sp					1529:1530	the name Paenibacillus paridis sp	1498:1530	the name Paenibacillus paridis sp	1498:1530	According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain py1325T clearly represents a novel species of the genus Paenibacillus, for which the name Paenibacillus paridis sp.
31967956	9	27	theme	DSM	1074:1076	arg1	19417T					1078:1083	Paenibacillus castaneae DSM 19417T	1050:1083	Paenibacillus castaneae DSM 19417T (97.5 %)	1050:1092	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	9	27	theme	DSM	1074:1076	arg1	%					1091:1091	97.5 %	1086:1091	97.5 %	1086:1091	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	9	28	theme	Paenibacillus	957:969	arg1	R-3T					978:981	Paenibacillus luteus R-3T	957:981	Paenibacillus luteus R-3T (99.0 %)	957:990	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	9	28	theme	Paenibacillus	957:969	arg1	%					989:989	99.0 %	984:989	99.0 %	984:989	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	0	29	theme	paridis	14:20	arg1	sp					22:23	Paenibacillus paridis sp	0:23	Paenibacillus paridis sp.	0:24	Paenibacillus paridis sp.
31967956	8	30	theme	polar	648:652	arg1	lipids					654:659	The polar lipids	644:659	The polar lipids	644:659	The polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phospholipids and two unidentified lipids.
31967956	14	31	theme	=CCTCC AB	1579:1587	arg1	py1325T					1570:1576	py1325T	1570:1576	py1325T (=CCTCC AB 2015220T=LMG 29068T)	1570:1608	The type strain is py1325T (=CCTCC AB 2015220T=LMG 29068T).
31967956	14	31	theme	=CCTCC AB	1579:1587	arg1	29068T					1602:1607	=CCTCC AB 2015220T=LMG 29068T	1579:1607	=CCTCC AB 2015220T=LMG 29068T	1579:1607	The type strain is py1325T (=CCTCC AB 2015220T=LMG 29068T).
31967956	0	32	theme	Paenibacillus	0:12	arg1	sp					22:23	Paenibacillus paridis sp	0:23	Paenibacillus paridis sp.	0:24	Paenibacillus paridis sp.
31967956	6	33	from	acid	527:530	arg1	peptidoglycan					549:561	the cell-wall peptidoglycan	535:561	the cell-wall peptidoglycan	535:561	The major respiratory quinone was MK-7 and the diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
31967956	3	34	theme	bacterial	190:198	arg1	strain					200:205	A Gram-reaction-positive, endospore-forming and rod-shaped bacterial strain	131:205	A Gram-reaction-positive, endospore-forming and rod-shaped bacterial strain	131:205	A Gram-reaction-positive, endospore-forming and rod-shaped bacterial strain, designated py1325T, was isolated from the root of Paris polyphylla Smith var.
31967956	6	35	theme	cell-wall	539:547	arg1	peptidoglycan					549:561	the cell-wall peptidoglycan	535:561	the cell-wall peptidoglycan	535:561	The major respiratory quinone was MK-7 and the diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
31967956	4	36	theme	taxonomic	370:378	arg1	characterization					380:395	a polyphasic taxonomic characterization	357:395	a polyphasic taxonomic characterization	357:395	yunnanensis collected from Yunnan Province, PR China, and subjected to a polyphasic taxonomic characterization.
31967956	9	37	theme	rRNA	862:865	arg1	analysis					881:888	16S rRNA gene sequence analysis	858:888	16S rRNA gene sequence analysis	858:888	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	4	38	theme	polyphasic	359:368	arg1	characterization					380:395	a polyphasic taxonomic characterization	357:395	a polyphasic taxonomic characterization	357:395	yunnanensis collected from Yunnan Province, PR China, and subjected to a polyphasic taxonomic characterization.
31967956	12	39	theme	paridis	1521:1527	arg1	sp					1529:1530	the name Paenibacillus paridis sp	1498:1530	the name Paenibacillus paridis sp	1498:1530	According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain py1325T clearly represents a novel species of the genus Paenibacillus, for which the name Paenibacillus paridis sp.
31967956	3	40	theme	var	281:283	arg1	root					250:253	the root	246:253	the root of Paris polyphylla Smith var	246:283	A Gram-reaction-positive, endospore-forming and rod-shaped bacterial strain, designated py1325T, was isolated from the root of Paris polyphylla Smith var.
31967956	12	41	theme	genus	1467:1471	arg1	species					1452:1458	a novel species	1444:1458	a novel species	1444:1458	According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain py1325T clearly represents a novel species of the genus Paenibacillus, for which the name Paenibacillus paridis sp.
31967956	10	42	theme	%	1270:1270	arg1	%					1286:1286	20.6-53.3 % and 79.9-93.6 %	1260:1286	20.6-53.3 % and 79.9-93.6 %	1260:1286	The digital DNA-DNA hybridization and average nucleotide identity values between py1325T and these species ranged 20.6-53.3 % and 79.9-93.6 %.
31967956	1	43	attach	isolated	64:71	arg1	root					82:85	the root	78:85	the root of Paris polyphylla Smith var	78:115	nov., an endophytic bacterial species isolated from the root of Paris polyphylla Smith var.
31967956	1	43	attach	isolated	64:71	arg2	species					56:62	an endophytic bacterial species	32:62	an endophytic bacterial species isolated from the root of Paris polyphylla Smith var	32:115	nov., an endophytic bacterial species isolated from the root of Paris polyphylla Smith var.
31967956	9	44	theme	sequence	872:879	arg1	analysis					881:888	16S rRNA gene sequence analysis	858:888	16S rRNA gene sequence analysis	858:888	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	9	45	theme	CCTCC	1022:1026	arg1	2016047T					1031:1038	Paenibacillus sinopodophylli CCTCC AB 2016047T	993:1038	Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %)	993:1047	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	9	45	theme	CCTCC	1022:1026	arg1	%					1046:1046	97.9 %	1041:1046	97.9 %	1041:1046	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	9	46	theme	analysis	881:888	arg1	results					847:853	The results	843:853	The results of 16S rRNA gene sequence analysis	843:888	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	11	47	theme	G+C	1293:1295	arg1	%					1336:1336	47.7 mol%	1328:1336	47.7 mol%	1328:1336	The G+C content of the genomic DNA was 47.7 mol%.
31967956	11	47	theme	G+C	1293:1295	arg1	content					1297:1303	The G+C content	1289:1303	The G+C content of the genomic DNA	1289:1322	The G+C content of the genomic DNA was 47.7 mol%.
31967956	6	48	theme	diamino	519:525	arg1	acid					527:530	the diagnostic diamino acid	504:530	the diagnostic diamino acid in the cell-wall peptidoglycan	504:561	The major respiratory quinone was MK-7 and the diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
31967956	6	48	theme	diamino	519:525	arg1	acid					587:590	meso-diaminopimelic acid	567:590	meso-diaminopimelic acid	567:590	The major respiratory quinone was MK-7 and the diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
31967956	12	49	theme	Paenibacillus	1473:1485	arg1	genus					1467:1471	the genus Paenibacillus	1463:1485	the genus Paenibacillus	1463:1485	According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain py1325T clearly represents a novel species of the genus Paenibacillus, for which the name Paenibacillus paridis sp.
31967956	9	50	theme	Paenibacillus	1098:1110	arg1	27297T					1129:1134	Paenibacillus endophyticus LMG 27297T	1098:1134	Paenibacillus endophyticus LMG 27297T (97.2 %)	1098:1143	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	9	50	theme	Paenibacillus	1098:1110	arg1	%					1142:1142	97.2 %	1137:1142	97.2 %	1137:1142	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	12	51	theme	name	1502:1505	arg1	sp					1529:1530	the name Paenibacillus paridis sp	1498:1530	the name Paenibacillus paridis sp	1498:1530	According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain py1325T clearly represents a novel species of the genus Paenibacillus, for which the name Paenibacillus paridis sp.
31967956	6	52	theme	diagnostic	508:517	arg1	acid					527:530	the diagnostic diamino acid	504:530	the diagnostic diamino acid in the cell-wall peptidoglycan	504:561	The major respiratory quinone was MK-7 and the diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
31967956	6	52	theme	diagnostic	508:517	arg1	acid					587:590	meso-diaminopimelic acid	567:590	meso-diaminopimelic acid	567:590	The major respiratory quinone was MK-7 and the diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
31967956	12	53	theme	novel	1446:1450	arg1	species					1452:1458	a novel species	1444:1458	a novel species	1444:1458	According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain py1325T clearly represents a novel species of the genus Paenibacillus, for which the name Paenibacillus paridis sp.
31967956	9	54	theme	endophyticus	1112:1123	arg1	27297T					1129:1134	Paenibacillus endophyticus LMG 27297T	1098:1134	Paenibacillus endophyticus LMG 27297T (97.2 %)	1098:1143	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	9	54	theme	endophyticus	1112:1123	arg1	%					1142:1142	97.2 %	1137:1142	97.2 %	1137:1142	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	11	55	theme	47.7 mol	1328:1335	arg1	%					1336:1336	47.7 mol%	1328:1336	47.7 mol%	1328:1336	The G+C content of the genomic DNA was 47.7 mol%.
31967956	11	55	theme	47.7 mol	1328:1335	arg1	content					1297:1303	The G+C content	1289:1303	The G+C content of the genomic DNA	1289:1322	The G+C content of the genomic DNA was 47.7 mol%.
31967956	8	56	theme	unidentified	822:833	arg1	lipids					835:840	two unidentified lipids	818:840	two unidentified lipids	818:840	The polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phospholipids and two unidentified lipids.
31967956	8	56	theme	unidentified	822:833	arg1	aminophospholipids					763:780	two unidentified aminophospholipids	746:780	two unidentified aminophospholipids	746:780	The polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phospholipids and two unidentified lipids.
31967956	9	57	theme	highest	903:909	arg1	levels					911:916	the highest levels	899:916	the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %)	899:1143	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	9	58	theme	LMG	1125:1127	arg1	27297T					1129:1134	Paenibacillus endophyticus LMG 27297T	1098:1134	Paenibacillus endophyticus LMG 27297T (97.2 %)	1098:1143	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	9	58	theme	LMG	1125:1127	arg1	%					1142:1142	97.2 %	1137:1142	97.2 %	1137:1142	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	1	59	theme	var	113:115	arg1	root					82:85	the root	78:85	the root of Paris polyphylla Smith var	78:115	nov., an endophytic bacterial species isolated from the root of Paris polyphylla Smith var.
31967956	9	60	theme	AB	1028:1029	arg1	2016047T					1031:1038	Paenibacillus sinopodophylli CCTCC AB 2016047T	993:1038	Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %)	993:1047	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	9	60	theme	AB	1028:1029	arg1	%					1046:1046	97.9 %	1041:1046	97.9 %	1041:1046	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	8	61	theme	unidentified	750:761	arg1	aminophospholipids					763:780	two unidentified aminophospholipids	746:780	two unidentified aminophospholipids	746:780	The polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phospholipids and two unidentified lipids.
31967956	8	61	theme	unidentified	750:761	arg1	lipids					835:840	two unidentified lipids	818:840	two unidentified lipids	818:840	The polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phospholipids and two unidentified lipids.
31967956	8	61	theme	unidentified	750:761	arg1	phospholipids					800:812	two unidentified phospholipids	783:812	two unidentified phospholipids	783:812	The polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phospholipids and two unidentified lipids.
31967956	8	61	theme	unidentified	750:761	arg1	phosphatidylglycerol					674:693	phosphatidylglycerol	674:693	phosphatidylglycerol	674:693	The polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phospholipids and two unidentified lipids.
31967956	12	62	theme	phylogenetic	1356:1367	arg1	evidence					1400:1407	the phylogenetic, phenotypic and chemotaxonomic evidence	1352:1407	the phylogenetic, phenotypic and chemotaxonomic evidence	1352:1407	According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain py1325T clearly represents a novel species of the genus Paenibacillus, for which the name Paenibacillus paridis sp.
31967956	9	63	theme	sinopodophylli	1007:1020	arg1	2016047T					1031:1038	Paenibacillus sinopodophylli CCTCC AB 2016047T	993:1038	Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %)	993:1047	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	9	63	theme	sinopodophylli	1007:1020	arg1	%					1046:1046	97.9 %	1041:1046	97.9 %	1041:1046	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	1	64	dep	species	56:62	arg1	nov.					26:29	nov.	26:29	nov.	26:29	nov., an endophytic bacterial species isolated from the root of Paris polyphylla Smith var.
31967956	10	65	theme	identity	1203:1210	arg1	values					1212:1217	The digital DNA-DNA hybridization and average nucleotide identity values	1146:1217	The digital DNA-DNA hybridization and average nucleotide identity values between py1325T and these species	1146:1251	The digital DNA-DNA hybridization and average nucleotide identity values between py1325T and these species ranged 20.6-53.3 % and 79.9-93.6 %.
31967956	10	66	theme	20.6-53.3 	1260:1269	arg1	%					1286:1286	20.6-53.3 % and 79.9-93.6 %	1260:1286	20.6-53.3 % and 79.9-93.6 %	1260:1286	The digital DNA-DNA hybridization and average nucleotide identity values between py1325T and these species ranged 20.6-53.3 % and 79.9-93.6 %.
31967956	10	67	theme	average	1184:1190	arg1	identity					1203:1210	average nucleotide identity	1184:1210	average nucleotide identity	1184:1210	The digital DNA-DNA hybridization and average nucleotide identity values between py1325T and these species ranged 20.6-53.3 % and 79.9-93.6 %.
31967956	5	68	theme	%	425:425	arg1	w/v					433:435	w/v	433:435	w/v	433:435	It grew optimally with 0-1 % NaCl (w/v), at pH 7 and at 30 °C.
31967956	5	68	theme	%	425:425	arg1	 NaCl					426:430	0-1 % NaCl	421:430	0-1 % NaCl (w/v)	421:436	It grew optimally with 0-1 % NaCl (w/v), at pH 7 and at 30 °C.
31967956	3	69	theme	Gram-reaction-positive	133:154	arg1	strain					200:205	A Gram-reaction-positive, endospore-forming and rod-shaped bacterial strain	131:205	A Gram-reaction-positive, endospore-forming and rod-shaped bacterial strain	131:205	A Gram-reaction-positive, endospore-forming and rod-shaped bacterial strain, designated py1325T, was isolated from the root of Paris polyphylla Smith var.
31967956	14	70	theme	type	1555:1558	arg1	py1325T					1570:1576	py1325T	1570:1576	py1325T (=CCTCC AB 2015220T=LMG 29068T)	1570:1608	The type strain is py1325T (=CCTCC AB 2015220T=LMG 29068T).
31967956	14	70	theme	type	1555:1558	arg1	strain					1560:1565	The type strain	1551:1565	The type strain	1551:1565	The type strain is py1325T (=CCTCC AB 2015220T=LMG 29068T).
31967956	10	71	theme	nucleotide	1192:1201	arg1	identity					1203:1210	average nucleotide identity	1184:1210	average nucleotide identity	1184:1210	The digital DNA-DNA hybridization and average nucleotide identity values between py1325T and these species ranged 20.6-53.3 % and 79.9-93.6 %.
31967956	9	72	theme	Paenibacillus	993:1005	arg1	2016047T					1031:1038	Paenibacillus sinopodophylli CCTCC AB 2016047T	993:1038	Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %)	993:1047	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	9	72	theme	Paenibacillus	993:1005	arg1	%					1046:1046	97.9 %	1041:1046	97.9 %	1041:1046	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	9	73	theme	sequence	921:928	arg1	similarity					930:939	sequence similarity	921:939	sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %)	921:1143	The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047T (97.9 %), Paenibacillus castaneae DSM 19417T (97.5 %) and Paenibacillus endophyticus LMG 27297T (97.2 %).
31967956	7	74	theme	fatty	612:616	arg1	 0					640:641	 0	640:641	 0	640:641	The major cellular fatty acid was anteiso-C15 : 0.
31967956	7	74	theme	fatty	612:616	arg1	acid					618:621	The major cellular fatty acid	593:621	The major cellular fatty acid	593:621	The major cellular fatty acid was anteiso-C15 : 0.
31967956	1	75	theme	endophytic	35:44	arg1	species					56:62	an endophytic bacterial species	32:62	an endophytic bacterial species isolated from the root of Paris polyphylla Smith var	32:115	nov., an endophytic bacterial species isolated from the root of Paris polyphylla Smith var.
31967956	4	76	theme	PR	330:331	arg1	Province					320:327	Province	320:327	Province	320:327	yunnanensis collected from Yunnan Province, PR China, and subjected to a polyphasic taxonomic characterization.
31967956	4	76	theme	PR	330:331	arg1	China					333:337	PR China	330:337	PR China	330:337	yunnanensis collected from Yunnan Province, PR China, and subjected to a polyphasic taxonomic characterization.
31967956	10	77	theme	digital	1150:1156	arg1	hybridization					1166:1178	digital DNA-DNA hybridization	1150:1178	digital DNA-DNA hybridization	1150:1178	The digital DNA-DNA hybridization and average nucleotide identity values between py1325T and these species ranged 20.6-53.3 % and 79.9-93.6 %.
34700334	6	0	theme	Lactobacillus	1101:1113	arg1	loss					1073:1076	the loss	1069:1076	the loss of Bifidobacterium and Lactobacillus	1069:1113	PSPAE prevented the loss of Bifidobacterium and Lactobacillus and inhibited the increase of Gammaproteobacteria and Helicobacter upon DSS treatment.
34700334	10	1	theme	colonization	1671:1682	arg1	exacerbated					1633:1643	exacerbated	1633:1643	exacerbated	1633:1643	Moreover, the pathogenic-dependent dysbiosis was exacerbated because of the increasing colonization of pathogens such as Helicobacter.
34700334	10	1	theme	colonization	1671:1682	arg1	dysbiosis					1619:1627	the pathogenic-dependent dysbiosis	1594:1627	the pathogenic-dependent dysbiosis	1594:1627	Moreover, the pathogenic-dependent dysbiosis was exacerbated because of the increasing colonization of pathogens such as Helicobacter.
34700334	9	2	theme	non-pathogenic	1514:1527	arg1	treatment					1529:1537	the non-pathogenic treatment	1510:1537	the non-pathogenic treatment	1510:1537	However, the non-pathogenic treatment could not attenuate intestinal inflammation.
34700334	3	3	theme	intestinal	733:742	arg1	homeostasis					744:754	intestinal homeostasis	733:754	intestinal homeostasis	733:754	We aimed to ascertain whether the purple sweet potato anthocyanin extract (PSPAE) modulation of gut microbiota in the dextran sodium sulphate (DSS) induced chronic colitis mouse model could result in the maintenance of intestinal homeostasis and protection against bacterial intestinal inflammation.
34700334	3	4	theme	extract	580:586	arg1	modulation					596:605	the purple sweet potato anthocyanin extract (PSPAE) modulation	544:605	the purple sweet potato anthocyanin extract (PSPAE) modulation of gut microbiota in the dextran sodium sulphate (DSS) induced chronic colitis mouse model	544:696	We aimed to ascertain whether the purple sweet potato anthocyanin extract (PSPAE) modulation of gut microbiota in the dextran sodium sulphate (DSS) induced chronic colitis mouse model could result in the maintenance of intestinal homeostasis and protection against bacterial intestinal inflammation.
34700334	3	5	theme	potato	561:566	arg1	modulation					596:605	the purple sweet potato anthocyanin extract (PSPAE) modulation	544:605	the purple sweet potato anthocyanin extract (PSPAE) modulation of gut microbiota in the dextran sodium sulphate (DSS) induced chronic colitis mouse model	544:696	We aimed to ascertain whether the purple sweet potato anthocyanin extract (PSPAE) modulation of gut microbiota in the dextran sodium sulphate (DSS) induced chronic colitis mouse model could result in the maintenance of intestinal homeostasis and protection against bacterial intestinal inflammation.
34700334	11	6	theme	bacterial	1951:1959	arg1	infection					1972:1980	bacterial intestinal infection	1951:1980	bacterial intestinal infection	1951:1980	The PSPAE exerted the modulation of gut microbiota to maintain the gut microbiome homeostasis in DSS-induced chronic colitis mice, which may help to propose a new treatment that combines efficacy and reduction of the possibility of bacterial intestinal infection.
34700334	6	7	theme	DSS	1187:1189	arg1	treatment					1191:1199	DSS treatment	1187:1199	DSS treatment	1187:1199	PSPAE prevented the loss of Bifidobacterium and Lactobacillus and inhibited the increase of Gammaproteobacteria and Helicobacter upon DSS treatment.
34700334	11	8	theme	possibility	1936:1946	arg1	efficacy					1906:1913	efficacy	1906:1913	efficacy	1906:1913	The PSPAE exerted the modulation of gut microbiota to maintain the gut microbiome homeostasis in DSS-induced chronic colitis mice, which may help to propose a new treatment that combines efficacy and reduction of the possibility of bacterial intestinal infection.
34700334	11	8	theme	possibility	1936:1946	arg1	reduction					1919:1927	reduction	1919:1927	reduction	1919:1927	The PSPAE exerted the modulation of gut microbiota to maintain the gut microbiome homeostasis in DSS-induced chronic colitis mice, which may help to propose a new treatment that combines efficacy and reduction of the possibility of bacterial intestinal infection.
34700334	2	9	theme	anthocyanins	326:337	arg1	ability					315:321	the ability	311:321	the ability of anthocyanins in modulating the gut microbiota	311:370	Although the ability of anthocyanins in modulating the gut microbiota has been reported, the relationship between the bacteria modulated by anthocyanins and intestinal inflammation has not been fully elucidated.
34700334	6	10	theme	Helicobacter	1169:1180	arg1	increase					1133:1140	the increase	1129:1140	the increase of Gammaproteobacteria and Helicobacter	1129:1180	PSPAE prevented the loss of Bifidobacterium and Lactobacillus and inhibited the increase of Gammaproteobacteria and Helicobacter upon DSS treatment.
34700334	3	11	theme	PSPAE	589:593	arg1	modulation					596:605	the purple sweet potato anthocyanin extract (PSPAE) modulation	544:605	the purple sweet potato anthocyanin extract (PSPAE) modulation of gut microbiota in the dextran sodium sulphate (DSS) induced chronic colitis mouse model	544:696	We aimed to ascertain whether the purple sweet potato anthocyanin extract (PSPAE) modulation of gut microbiota in the dextran sodium sulphate (DSS) induced chronic colitis mouse model could result in the maintenance of intestinal homeostasis and protection against bacterial intestinal inflammation.
34700334	8	12	theme	non-pathogenic	1364:1377	arg1	treatments					1379:1388	non-pathogenic treatments	1364:1388	non-pathogenic treatments	1364:1388	Both PSPAE treatment and non-pathogenic treatments modified the colonic expression of mouse tight junction proteins and maintained the architecture of the colon.
34700334	11	13	theme	infection	1972:1980	arg1	possibility					1936:1946	the possibility	1932:1946	the possibility of bacterial intestinal infection	1932:1980	The PSPAE exerted the modulation of gut microbiota to maintain the gut microbiome homeostasis in DSS-induced chronic colitis mice, which may help to propose a new treatment that combines efficacy and reduction of the possibility of bacterial intestinal infection.
34700334	0	14	theme	bacterial	141:149	arg1	effects					18:24	Anti-inflammatory effects	0:24	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.	0:171	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.
34700334	0	14	theme	bacterial	141:149	arg1	infection					162:170	attenuated bacterial intestinal infection	130:170	attenuated bacterial intestinal infection	130:170	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.
34700334	1	15	theme	beneficial	239:248	arg1	effects					250:256	their beneficial effects	233:256	their beneficial effects on human inflammatory bowel diseases (IBD)	233:299	Purple sweet potato anthocyanins have been acknowledged for their beneficial effects on human inflammatory bowel diseases (IBD).
34700334	3	16	theme	intestinal	789:798	arg1	inflammation					800:811	bacterial intestinal inflammation	779:811	bacterial intestinal inflammation	779:811	We aimed to ascertain whether the purple sweet potato anthocyanin extract (PSPAE) modulation of gut microbiota in the dextran sodium sulphate (DSS) induced chronic colitis mouse model could result in the maintenance of intestinal homeostasis and protection against bacterial intestinal inflammation.
34700334	3	17	theme	gut	610:612	arg1	microbiota					614:623	gut microbiota	610:623	gut microbiota	610:623	We aimed to ascertain whether the purple sweet potato anthocyanin extract (PSPAE) modulation of gut microbiota in the dextran sodium sulphate (DSS) induced chronic colitis mouse model could result in the maintenance of intestinal homeostasis and protection against bacterial intestinal inflammation.
34700334	4	18	theme	mg	929:930	arg1	gavage					918:923	gavage	918:923	gavage (20 mg kg-1)	918:936	Chronic colitis was induced by adding DSS in drinking water while administering the mice with PSPAE via gavage (20 mg kg-1).
34700334	4	18	theme	mg	929:930	arg1	kg-1					932:935	20 mg kg-1	926:935	20 mg kg-1	926:935	Chronic colitis was induced by adding DSS in drinking water while administering the mice with PSPAE via gavage (20 mg kg-1).
34700334	8	19	theme	colonic	1403:1409	arg1	expression					1411:1420	the colonic expression	1399:1420	the colonic expression of mouse tight junction proteins	1399:1453	Both PSPAE treatment and non-pathogenic treatments modified the colonic expression of mouse tight junction proteins and maintained the architecture of the colon.
34700334	7	20	with	treatment	1296:1304	arg1	antibiotics					1326:1336	broad-spectrum antibiotics	1311:1336	broad-spectrum antibiotics	1311:1336	The non-pathogenic-dependent and pathogenic-dependent microenvironments were established upon treatment with broad-spectrum antibiotics.
34700334	3	21	from	modulation	596:605	arg1	model					692:696	the dextran sodium sulphate (DSS) induced chronic colitis mouse model	628:696	the dextran sodium sulphate (DSS) induced chronic colitis mouse model	628:696	We aimed to ascertain whether the purple sweet potato anthocyanin extract (PSPAE) modulation of gut microbiota in the dextran sodium sulphate (DSS) induced chronic colitis mouse model could result in the maintenance of intestinal homeostasis and protection against bacterial intestinal inflammation.
34700334	8	22	theme	tight	1431:1435	arg1	proteins					1446:1453	mouse tight junction proteins	1425:1453	mouse tight junction proteins	1425:1453	Both PSPAE treatment and non-pathogenic treatments modified the colonic expression of mouse tight junction proteins and maintained the architecture of the colon.
34700334	11	23	theme	DSS-induced	1816:1826	arg1	colitis					1836:1842	DSS-induced chronic colitis	1816:1842	DSS-induced chronic colitis mice	1816:1847	The PSPAE exerted the modulation of gut microbiota to maintain the gut microbiome homeostasis in DSS-induced chronic colitis mice, which may help to propose a new treatment that combines efficacy and reduction of the possibility of bacterial intestinal infection.
34700334	1	24	theme	sweet	180:184	arg1	anthocyanins					193:204	Purple sweet potato anthocyanins	173:204	Purple sweet potato anthocyanins	173:204	Purple sweet potato anthocyanins have been acknowledged for their beneficial effects on human inflammatory bowel diseases (IBD).
34700334	8	25	theme	proteins	1446:1453	arg1	expression					1411:1420	the colonic expression	1399:1420	the colonic expression of mouse tight junction proteins	1399:1453	Both PSPAE treatment and non-pathogenic treatments modified the colonic expression of mouse tight junction proteins and maintained the architecture of the colon.
34700334	5	26	theme	gut	971:973	arg1	composition					986:996	gut microbiota composition	971:996	gut microbiota composition	971:996	Effects on colon tissue damage, gut microbiota composition, tight junction protein, and cytokines were evaluated.
34700334	4	27	theme	Chronic	814:820	arg1	colitis					822:828	Chronic colitis	814:828	Chronic colitis	814:828	Chronic colitis was induced by adding DSS in drinking water while administering the mice with PSPAE via gavage (20 mg kg-1).
34700334	7	28	theme	pathogenic-dependent	1235:1254	arg1	microenvironments					1256:1272	The non-pathogenic-dependent and pathogenic-dependent microenvironments	1202:1272	The non-pathogenic-dependent and pathogenic-dependent microenvironments	1202:1272	The non-pathogenic-dependent and pathogenic-dependent microenvironments were established upon treatment with broad-spectrum antibiotics.
34700334	11	29	from	homeostasis	1801:1811	arg1	mice					1844:1847	DSS-induced chronic colitis mice	1816:1847	DSS-induced chronic colitis mice	1816:1847	The PSPAE exerted the modulation of gut microbiota to maintain the gut microbiome homeostasis in DSS-induced chronic colitis mice, which may help to propose a new treatment that combines efficacy and reduction of the possibility of bacterial intestinal infection.
34700334	11	30	theme	chronic	1828:1834	arg1	colitis					1836:1842	DSS-induced chronic colitis	1816:1842	DSS-induced chronic colitis mice	1816:1847	The PSPAE exerted the modulation of gut microbiota to maintain the gut microbiome homeostasis in DSS-induced chronic colitis mice, which may help to propose a new treatment that combines efficacy and reduction of the possibility of bacterial intestinal infection.
34700334	7	31	theme	non-pathogenic-dependent	1206:1229	arg1	microenvironments					1256:1272	The non-pathogenic-dependent and pathogenic-dependent microenvironments	1202:1272	The non-pathogenic-dependent and pathogenic-dependent microenvironments	1202:1272	The non-pathogenic-dependent and pathogenic-dependent microenvironments were established upon treatment with broad-spectrum antibiotics.
34700334	5	32	theme	colon	950:954	arg1	damage					963:968	colon tissue damage	950:968	colon tissue damage	950:968	Effects on colon tissue damage, gut microbiota composition, tight junction protein, and cytokines were evaluated.
34700334	2	33	theme	intestinal	459:468	arg1	inflammation					470:481	intestinal inflammation	459:481	intestinal inflammation	459:481	Although the ability of anthocyanins in modulating the gut microbiota has been reported, the relationship between the bacteria modulated by anthocyanins and intestinal inflammation has not been fully elucidated.
34700334	3	34	theme	induced	662:668	arg1	model					692:696	the dextran sodium sulphate (DSS) induced chronic colitis mouse model	628:696	the dextran sodium sulphate (DSS) induced chronic colitis mouse model	628:696	We aimed to ascertain whether the purple sweet potato anthocyanin extract (PSPAE) modulation of gut microbiota in the dextran sodium sulphate (DSS) induced chronic colitis mouse model could result in the maintenance of intestinal homeostasis and protection against bacterial intestinal inflammation.
34700334	0	35	theme	sweet	36:40	arg1	extract					61:67	purple sweet potato anthocyanin extract	29:67	purple sweet potato anthocyanin extract	29:67	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.
34700334	10	36	theme	increasing	1660:1669	arg1	colonization					1671:1682	the increasing colonization	1656:1682	the increasing colonization of pathogens such as Helicobacter	1656:1716	Moreover, the pathogenic-dependent dysbiosis was exacerbated because of the increasing colonization of pathogens such as Helicobacter.
34700334	0	37	theme	anthocyanin	49:59	arg1	extract					61:67	purple sweet potato anthocyanin extract	29:67	purple sweet potato anthocyanin extract	29:67	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.
34700334	5	38	theme	tight	999:1003	arg1	protein					1014:1020	tight junction protein	999:1020	tight junction protein	999:1020	Effects on colon tissue damage, gut microbiota composition, tight junction protein, and cytokines were evaluated.
34700334	3	39	theme	colitis	678:684	arg1	model					692:696	the dextran sodium sulphate (DSS) induced chronic colitis mouse model	628:696	the dextran sodium sulphate (DSS) induced chronic colitis mouse model	628:696	We aimed to ascertain whether the purple sweet potato anthocyanin extract (PSPAE) modulation of gut microbiota in the dextran sodium sulphate (DSS) induced chronic colitis mouse model could result in the maintenance of intestinal homeostasis and protection against bacterial intestinal inflammation.
34700334	1	40	theme	human	261:265	arg1	diseases					286:293	human inflammatory bowel diseases	261:293	human inflammatory bowel diseases (IBD)	261:299	Purple sweet potato anthocyanins have been acknowledged for their beneficial effects on human inflammatory bowel diseases (IBD).
34700334	1	40	theme	human	261:265	arg1	IBD					296:298	IBD	296:298	IBD	296:298	Purple sweet potato anthocyanins have been acknowledged for their beneficial effects on human inflammatory bowel diseases (IBD).
34700334	11	41	theme	microbiome	1790:1799	arg1	homeostasis					1801:1811	the gut microbiome homeostasis	1782:1811	the gut microbiome homeostasis in DSS-induced chronic colitis mice, which may help to propose a new treatment that combines efficacy and reduction of the possibility of bacterial intestinal infection	1782:1980	The PSPAE exerted the modulation of gut microbiota to maintain the gut microbiome homeostasis in DSS-induced chronic colitis mice, which may help to propose a new treatment that combines efficacy and reduction of the possibility of bacterial intestinal infection.
34700334	11	42	theme	gut	1755:1757	arg1	microbiota					1759:1768	gut microbiota	1755:1768	gut microbiota	1755:1768	The PSPAE exerted the modulation of gut microbiota to maintain the gut microbiome homeostasis in DSS-induced chronic colitis mice, which may help to propose a new treatment that combines efficacy and reduction of the possibility of bacterial intestinal infection.
34700334	1	43	theme	bowel	280:284	arg1	diseases					286:293	human inflammatory bowel diseases	261:293	human inflammatory bowel diseases (IBD)	261:299	Purple sweet potato anthocyanins have been acknowledged for their beneficial effects on human inflammatory bowel diseases (IBD).
34700334	1	43	theme	bowel	280:284	arg1	IBD					296:298	IBD	296:298	IBD	296:298	Purple sweet potato anthocyanins have been acknowledged for their beneficial effects on human inflammatory bowel diseases (IBD).
34700334	3	44	theme	anthocyanin	568:578	arg1	modulation					596:605	the purple sweet potato anthocyanin extract (PSPAE) modulation	544:605	the purple sweet potato anthocyanin extract (PSPAE) modulation of gut microbiota in the dextran sodium sulphate (DSS) induced chronic colitis mouse model	544:696	We aimed to ascertain whether the purple sweet potato anthocyanin extract (PSPAE) modulation of gut microbiota in the dextran sodium sulphate (DSS) induced chronic colitis mouse model could result in the maintenance of intestinal homeostasis and protection against bacterial intestinal inflammation.
34700334	3	45	theme	homeostasis	744:754	arg1	maintenance					718:728	the maintenance	714:728	the maintenance of intestinal homeostasis and protection against bacterial intestinal inflammation	714:811	We aimed to ascertain whether the purple sweet potato anthocyanin extract (PSPAE) modulation of gut microbiota in the dextran sodium sulphate (DSS) induced chronic colitis mouse model could result in the maintenance of intestinal homeostasis and protection against bacterial intestinal inflammation.
34700334	3	46	theme	sweet	555:559	arg1	modulation					596:605	the purple sweet potato anthocyanin extract (PSPAE) modulation	544:605	the purple sweet potato anthocyanin extract (PSPAE) modulation of gut microbiota in the dextran sodium sulphate (DSS) induced chronic colitis mouse model	544:696	We aimed to ascertain whether the purple sweet potato anthocyanin extract (PSPAE) modulation of gut microbiota in the dextran sodium sulphate (DSS) induced chronic colitis mouse model could result in the maintenance of intestinal homeostasis and protection against bacterial intestinal inflammation.
34700334	1	47	theme	potato	186:191	arg1	anthocyanins					193:204	Purple sweet potato anthocyanins	173:204	Purple sweet potato anthocyanins	173:204	Purple sweet potato anthocyanins have been acknowledged for their beneficial effects on human inflammatory bowel diseases (IBD).
34700334	9	48	theme	intestinal	1559:1568	arg1	inflammation					1570:1581	intestinal inflammation	1559:1581	intestinal inflammation	1559:1581	However, the non-pathogenic treatment could not attenuate intestinal inflammation.
34700334	3	49	theme	bacterial	779:787	arg1	inflammation					800:811	bacterial intestinal inflammation	779:811	bacterial intestinal inflammation	779:811	We aimed to ascertain whether the purple sweet potato anthocyanin extract (PSPAE) modulation of gut microbiota in the dextran sodium sulphate (DSS) induced chronic colitis mouse model could result in the maintenance of intestinal homeostasis and protection against bacterial intestinal inflammation.
34700334	0	50	theme	attenuated	130:139	arg1	effects					18:24	Anti-inflammatory effects	0:24	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.	0:171	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.
34700334	0	50	theme	attenuated	130:139	arg1	infection					162:170	attenuated bacterial intestinal infection	130:170	attenuated bacterial intestinal infection	130:170	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.
34700334	8	51	theme	PSPAE	1344:1348	arg1	treatment					1350:1358	PSPAE treatment	1344:1358	PSPAE treatment	1344:1358	Both PSPAE treatment and non-pathogenic treatments modified the colonic expression of mouse tight junction proteins and maintained the architecture of the colon.
34700334	3	52	theme	protection	760:769	arg1	maintenance					718:728	the maintenance	714:728	the maintenance of intestinal homeostasis and protection against bacterial intestinal inflammation	714:811	We aimed to ascertain whether the purple sweet potato anthocyanin extract (PSPAE) modulation of gut microbiota in the dextran sodium sulphate (DSS) induced chronic colitis mouse model could result in the maintenance of intestinal homeostasis and protection against bacterial intestinal inflammation.
34700334	0	53	theme	intestinal	151:160	arg1	effects					18:24	Anti-inflammatory effects	0:24	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.	0:171	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.
34700334	0	53	theme	intestinal	151:160	arg1	infection					162:170	attenuated bacterial intestinal infection	130:170	attenuated bacterial intestinal infection	130:170	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.
34700334	3	54	theme	microbiota	614:623	arg1	modulation					596:605	the purple sweet potato anthocyanin extract (PSPAE) modulation	544:605	the purple sweet potato anthocyanin extract (PSPAE) modulation of gut microbiota in the dextran sodium sulphate (DSS) induced chronic colitis mouse model	544:696	We aimed to ascertain whether the purple sweet potato anthocyanin extract (PSPAE) modulation of gut microbiota in the dextran sodium sulphate (DSS) induced chronic colitis mouse model could result in the maintenance of intestinal homeostasis and protection against bacterial intestinal inflammation.
34700334	11	55	theme	intestinal	1961:1970	arg1	infection					1972:1980	bacterial intestinal infection	1951:1980	bacterial intestinal infection	1951:1980	The PSPAE exerted the modulation of gut microbiota to maintain the gut microbiome homeostasis in DSS-induced chronic colitis mice, which may help to propose a new treatment that combines efficacy and reduction of the possibility of bacterial intestinal infection.
34700334	1	56	from	effects	250:256	arg1	diseases					286:293	human inflammatory bowel diseases	261:293	human inflammatory bowel diseases (IBD)	261:299	Purple sweet potato anthocyanins have been acknowledged for their beneficial effects on human inflammatory bowel diseases (IBD).
34700334	1	56	from	effects	250:256	arg1	IBD					296:298	IBD	296:298	IBD	296:298	Purple sweet potato anthocyanins have been acknowledged for their beneficial effects on human inflammatory bowel diseases (IBD).
34700334	0	57	theme	DSS-induced	72:82	arg1	colitis					84:90	DSS-induced colitis	72:90	DSS-induced colitis	72:90	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.
34700334	4	58	with	mice	898:901	arg1	PSPAE					908:912	PSPAE	908:912	PSPAE	908:912	Chronic colitis was induced by adding DSS in drinking water while administering the mice with PSPAE via gavage (20 mg kg-1).
34700334	11	59	theme	new	1878:1880	arg1	treatment					1882:1890	a new treatment	1876:1890	a new treatment that combines efficacy and reduction of the possibility of bacterial intestinal infection	1876:1980	The PSPAE exerted the modulation of gut microbiota to maintain the gut microbiome homeostasis in DSS-induced chronic colitis mice, which may help to propose a new treatment that combines efficacy and reduction of the possibility of bacterial intestinal infection.
34700334	1	60	theme	Purple	173:178	arg1	anthocyanins					193:204	Purple sweet potato anthocyanins	173:204	Purple sweet potato anthocyanins	173:204	Purple sweet potato anthocyanins have been acknowledged for their beneficial effects on human inflammatory bowel diseases (IBD).
34700334	0	61	from	effects	18:24	arg1	colitis					84:90	DSS-induced colitis	72:90	DSS-induced colitis	72:90	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.
34700334	8	62	theme	mouse	1425:1429	arg1	proteins					1446:1453	mouse tight junction proteins	1425:1453	mouse tight junction proteins	1425:1453	Both PSPAE treatment and non-pathogenic treatments modified the colonic expression of mouse tight junction proteins and maintained the architecture of the colon.
34700334	8	63	theme	junction	1437:1444	arg1	proteins					1446:1453	mouse tight junction proteins	1425:1453	mouse tight junction proteins	1425:1453	Both PSPAE treatment and non-pathogenic treatments modified the colonic expression of mouse tight junction proteins and maintained the architecture of the colon.
34700334	0	64	theme	Anti-inflammatory	0:16	arg1	modulation					93:102	modulation	93:102	modulation of commensal bacteria	93:124	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.
34700334	0	64	theme	Anti-inflammatory	0:16	arg1	effects					18:24	Anti-inflammatory effects	0:24	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.	0:171	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.
34700334	0	64	theme	Anti-inflammatory	0:16	arg1	infection					162:170	attenuated bacterial intestinal infection	130:170	attenuated bacterial intestinal infection	130:170	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.
34700334	0	65	theme	bacteria	117:124	arg1	infection					162:170	attenuated bacterial intestinal infection	130:170	attenuated bacterial intestinal infection	130:170	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.
34700334	0	65	theme	bacteria	117:124	arg1	modulation					93:102	modulation	93:102	modulation of commensal bacteria	93:124	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.
34700334	0	65	theme	bacteria	117:124	arg1	effects					18:24	Anti-inflammatory effects	0:24	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.	0:171	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.
34700334	5	66	theme	microbiota	975:984	arg1	composition					986:996	gut microbiota composition	971:996	gut microbiota composition	971:996	Effects on colon tissue damage, gut microbiota composition, tight junction protein, and cytokines were evaluated.
34700334	6	67	theme	Bifidobacterium	1081:1095	arg1	loss					1073:1076	the loss	1069:1076	the loss of Bifidobacterium and Lactobacillus	1069:1113	PSPAE prevented the loss of Bifidobacterium and Lactobacillus and inhibited the increase of Gammaproteobacteria and Helicobacter upon DSS treatment.
34700334	5	68	from	Effects	939:945	arg1	damage					963:968	colon tissue damage	950:968	colon tissue damage	950:968	Effects on colon tissue damage, gut microbiota composition, tight junction protein, and cytokines were evaluated.
34700334	5	68	from	Effects	939:945	arg1	cytokines					1027:1035	cytokines	1027:1035	cytokines	1027:1035	Effects on colon tissue damage, gut microbiota composition, tight junction protein, and cytokines were evaluated.
34700334	5	68	from	Effects	939:945	arg1	protein					1014:1020	tight junction protein	999:1020	tight junction protein	999:1020	Effects on colon tissue damage, gut microbiota composition, tight junction protein, and cytokines were evaluated.
34700334	5	68	from	Effects	939:945	arg1	composition					986:996	gut microbiota composition	971:996	gut microbiota composition	971:996	Effects on colon tissue damage, gut microbiota composition, tight junction protein, and cytokines were evaluated.
34700334	0	69	theme	purple	29:34	arg1	extract					61:67	purple sweet potato anthocyanin extract	29:67	purple sweet potato anthocyanin extract	29:67	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.
34700334	0	70	dep	effects	18:24	arg1	modulation					93:102	modulation	93:102	modulation of commensal bacteria	93:124	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.
34700334	0	70	dep	effects	18:24	arg1	infection					162:170	attenuated bacterial intestinal infection	130:170	attenuated bacterial intestinal infection	130:170	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.
34700334	0	70	dep	effects	18:24	arg1	effects					18:24	Anti-inflammatory effects	0:24	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.	0:171	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.
34700334	5	71	theme	tissue	956:961	arg1	damage					963:968	colon tissue damage	950:968	colon tissue damage	950:968	Effects on colon tissue damage, gut microbiota composition, tight junction protein, and cytokines were evaluated.
34700334	0	72	theme	potato	42:47	arg1	extract					61:67	purple sweet potato anthocyanin extract	29:67	purple sweet potato anthocyanin extract	29:67	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.
34700334	10	73	theme	pathogenic-dependent	1598:1617	arg1	exacerbated					1633:1643	exacerbated	1633:1643	exacerbated	1633:1643	Moreover, the pathogenic-dependent dysbiosis was exacerbated because of the increasing colonization of pathogens such as Helicobacter.
34700334	10	73	theme	pathogenic-dependent	1598:1617	arg1	dysbiosis					1619:1627	the pathogenic-dependent dysbiosis	1594:1627	the pathogenic-dependent dysbiosis	1594:1627	Moreover, the pathogenic-dependent dysbiosis was exacerbated because of the increasing colonization of pathogens such as Helicobacter.
34700334	11	74	theme	colitis	1836:1842	arg1	mice					1844:1847	DSS-induced chronic colitis mice	1816:1847	DSS-induced chronic colitis mice	1816:1847	The PSPAE exerted the modulation of gut microbiota to maintain the gut microbiome homeostasis in DSS-induced chronic colitis mice, which may help to propose a new treatment that combines efficacy and reduction of the possibility of bacterial intestinal infection.
34700334	8	75	theme	colon	1494:1498	arg1	architecture					1474:1485	the architecture	1470:1485	the architecture of the colon	1470:1498	Both PSPAE treatment and non-pathogenic treatments modified the colonic expression of mouse tight junction proteins and maintained the architecture of the colon.
34700334	0	76	theme	commensal	107:115	arg1	bacteria					117:124	commensal bacteria	107:124	commensal bacteria	107:124	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.
34700334	0	77	theme	extract	61:67	arg1	modulation					93:102	modulation	93:102	modulation of commensal bacteria	93:124	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.
34700334	0	77	theme	extract	61:67	arg1	effects					18:24	Anti-inflammatory effects	0:24	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.	0:171	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.
34700334	0	77	theme	extract	61:67	arg1	infection					162:170	attenuated bacterial intestinal infection	130:170	attenuated bacterial intestinal infection	130:170	Anti-inflammatory effects of purple sweet potato anthocyanin extract in DSS-induced colitis: modulation of commensal bacteria and attenuated bacterial intestinal infection.
34700334	6	78	theme	Gammaproteobacteria	1145:1163	arg1	increase					1133:1140	the increase	1129:1140	the increase of Gammaproteobacteria and Helicobacter	1129:1180	PSPAE prevented the loss of Bifidobacterium and Lactobacillus and inhibited the increase of Gammaproteobacteria and Helicobacter upon DSS treatment.
34700334	3	79	theme	mouse	686:690	arg1	model					692:696	the dextran sodium sulphate (DSS) induced chronic colitis mouse model	628:696	the dextran sodium sulphate (DSS) induced chronic colitis mouse model	628:696	We aimed to ascertain whether the purple sweet potato anthocyanin extract (PSPAE) modulation of gut microbiota in the dextran sodium sulphate (DSS) induced chronic colitis mouse model could result in the maintenance of intestinal homeostasis and protection against bacterial intestinal inflammation.
34700334	2	80	theme	gut	357:359	arg1	microbiota					361:370	the gut microbiota	353:370	the gut microbiota	353:370	Although the ability of anthocyanins in modulating the gut microbiota has been reported, the relationship between the bacteria modulated by anthocyanins and intestinal inflammation has not been fully elucidated.
34700334	3	81	theme	chronic	670:676	arg1	model					692:696	the dextran sodium sulphate (DSS) induced chronic colitis mouse model	628:696	the dextran sodium sulphate (DSS) induced chronic colitis mouse model	628:696	We aimed to ascertain whether the purple sweet potato anthocyanin extract (PSPAE) modulation of gut microbiota in the dextran sodium sulphate (DSS) induced chronic colitis mouse model could result in the maintenance of intestinal homeostasis and protection against bacterial intestinal inflammation.
34700334	11	82	theme	microbiota	1759:1768	arg1	modulation					1741:1750	the modulation	1737:1750	the modulation of gut microbiota to maintain the gut microbiome homeostasis in DSS-induced chronic colitis mice, which may help to propose a new treatment that combines efficacy and reduction of the possibility of bacterial intestinal infection	1737:1980	The PSPAE exerted the modulation of gut microbiota to maintain the gut microbiome homeostasis in DSS-induced chronic colitis mice, which may help to propose a new treatment that combines efficacy and reduction of the possibility of bacterial intestinal infection.
34700334	5	83	theme	junction	1005:1012	arg1	protein					1014:1020	tight junction protein	999:1020	tight junction protein	999:1020	Effects on colon tissue damage, gut microbiota composition, tight junction protein, and cytokines were evaluated.
34700334	10	84	theme	pathogens	1687:1695	arg1	colonization					1671:1682	the increasing colonization	1656:1682	the increasing colonization of pathogens such as Helicobacter	1656:1716	Moreover, the pathogenic-dependent dysbiosis was exacerbated because of the increasing colonization of pathogens such as Helicobacter.
34700334	11	85	theme	gut	1786:1788	arg1	homeostasis					1801:1811	the gut microbiome homeostasis	1782:1811	the gut microbiome homeostasis in DSS-induced chronic colitis mice, which may help to propose a new treatment that combines efficacy and reduction of the possibility of bacterial intestinal infection	1782:1980	The PSPAE exerted the modulation of gut microbiota to maintain the gut microbiome homeostasis in DSS-induced chronic colitis mice, which may help to propose a new treatment that combines efficacy and reduction of the possibility of bacterial intestinal infection.
34700334	3	86	theme	purple	548:553	arg1	modulation					596:605	the purple sweet potato anthocyanin extract (PSPAE) modulation	544:605	the purple sweet potato anthocyanin extract (PSPAE) modulation of gut microbiota in the dextran sodium sulphate (DSS) induced chronic colitis mouse model	544:696	We aimed to ascertain whether the purple sweet potato anthocyanin extract (PSPAE) modulation of gut microbiota in the dextran sodium sulphate (DSS) induced chronic colitis mouse model could result in the maintenance of intestinal homeostasis and protection against bacterial intestinal inflammation.
34700334	7	87	theme	broad-spectrum	1311:1324	arg1	antibiotics					1326:1336	broad-spectrum antibiotics	1311:1336	broad-spectrum antibiotics	1311:1336	The non-pathogenic-dependent and pathogenic-dependent microenvironments were established upon treatment with broad-spectrum antibiotics.
34700334	1	88	theme	inflammatory	267:278	arg1	diseases					286:293	human inflammatory bowel diseases	261:293	human inflammatory bowel diseases (IBD)	261:299	Purple sweet potato anthocyanins have been acknowledged for their beneficial effects on human inflammatory bowel diseases (IBD).
34700334	1	88	theme	inflammatory	267:278	arg1	IBD					296:298	IBD	296:298	IBD	296:298	Purple sweet potato anthocyanins have been acknowledged for their beneficial effects on human inflammatory bowel diseases (IBD).
33072135	6	0	theme	1H	822:823	arg1	spectroscopy					829:840	1H NMR spectroscopy	822:840	1H NMR spectroscopy	822:840	Meanwhile, feces samples were analyzed with 1H NMR spectroscopy to investigate the metabolic alterations.
33072135	10	1	theme	acid	1633:1636	arg1	metabolism					1638:1647	amino acid metabolism	1627:1647	amino acid metabolism	1627:1647	Metabolic data further revealed that BBR induced metabolic changes in feces focus on regulating glycometabolism, SCFA metabolism and amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are the products of interactions between the host and the microbial community.
33072135	8	2	from	composition	1090:1100	arg1	mice					1131:1134	AOM/DSS mice	1123:1134	AOM/DSS mice	1123:1134	Moreover, BBR altered the composition of gut microbiota in AOM/DSS mice obviously, which were characterized by a decrease of Actinobacteria and Verrucomicrobia significantly at the phylum level.
33072135	4	3	theme	sodium	527:532	arg1	DSS					543:545	DSS	543:545	DSS	543:545	First, azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse was used as CRC animal model, then the degree of colorectal carcinogenesis in AOM/DSS mice with or without BBR administration was measured.
33072135	4	3	theme	sodium	527:532	arg1	sulfate					534:540	azoxymethane (AOM)/dextran sodium sulfate	500:540	azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse	500:552	First, azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse was used as CRC animal model, then the degree of colorectal carcinogenesis in AOM/DSS mice with or without BBR administration was measured.
33072135	7	4	theme	better	1029:1034	arg1	development					940:950	intestinal tumor development	923:950	intestinal tumor development with lower macroscopic polyps	923:980	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	7	4	theme	better	1029:1034	arg1	expression					992:1001	ki-67 expression	986:1001	ki-67 expression of intestinal tissue	986:1022	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	7	4	theme	better	1029:1034	arg1	result					889:894	a result	887:894	a result	887:894	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	7	4	theme	better	1029:1034	arg1	morphology					1044:1053	better colonic morphology	1029:1053	better colonic morphology in mice	1029:1061	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	3	5	from	effects	470:476	arg1	CRC					488:490	CRC	488:490	CRC	488:490	Herein, we investigated the therapeutic effects of BBR on CRC from the perspective of gut microbiota and metabolic alterations, which can provide a holistic view to understand the effects of BBR on CRC.
33072135	11	6	theme	new	1937:1939	arg1	insight					1941:1947	new insight	1937:1947	new insight into the inhibition effects of BBR on CRC	1937:1989	This study showed that BBR induced alterations in microbiota and metabolic in AOM/DSS mice, which might providing new insight into the inhibition effects of BBR on CRC.
33072135	5	7	dep	composition	698:708	arg1	The					694:696	The	694:696	The	694:696	The composition and abundance of gut microbiota was investigated by using 16S rRNA.
33072135	4	8	theme	animal	570:575	arg1	mouse					548:552	azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse	500:552	azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse	500:552	First, azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse was used as CRC animal model, then the degree of colorectal carcinogenesis in AOM/DSS mice with or without BBR administration was measured.
33072135	4	8	theme	animal	570:575	arg1	model					577:581	CRC animal model	566:581	CRC animal model	566:581	First, azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse was used as CRC animal model, then the degree of colorectal carcinogenesis in AOM/DSS mice with or without BBR administration was measured.
33072135	2	9	theme	BBR	249:251	arg1	mechanism					236:244	the mechanism	232:244	the mechanism of BBR on CRC	232:258	However, the mechanism of BBR on CRC also remains largely unknown.
33072135	7	10	theme	intestinal	1006:1015	arg1	tissue					1017:1022	intestinal tissue	1006:1022	intestinal tissue	1006:1022	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	11	11	theme	inhibition	1958:1967	arg1	effects					1969:1975	the inhibition effects	1954:1975	the inhibition effects of BBR on CRC	1954:1989	This study showed that BBR induced alterations in microbiota and metabolic in AOM/DSS mice, which might providing new insight into the inhibition effects of BBR on CRC.
33072135	8	12	theme	microbiota	1109:1118	arg1	composition					1090:1100	the composition	1086:1100	the composition of gut microbiota in AOM/DSS mice	1086:1134	Moreover, BBR altered the composition of gut microbiota in AOM/DSS mice obviously, which were characterized by a decrease of Actinobacteria and Verrucomicrobia significantly at the phylum level.
33072135	11	13	theme	BBR	1980:1982	arg1	effects					1969:1975	the inhibition effects	1954:1975	the inhibition effects of BBR on CRC	1954:1989	This study showed that BBR induced alterations in microbiota and metabolic in AOM/DSS mice, which might providing new insight into the inhibition effects of BBR on CRC.
33072135	10	14	theme	Metabolic	1494:1502	arg1	data					1504:1507	Metabolic data	1494:1507	Metabolic data	1494:1507	Metabolic data further revealed that BBR induced metabolic changes in feces focus on regulating glycometabolism, SCFA metabolism and amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are the products of interactions between the host and the microbial community.
33072135	1	15	theme	colorectal	198:207	arg1	CRC					217:219	CRC	217:219	CRC	217:219	Berberine (BBR) has been reported that it has effects on inhibiting colorectal cancer (CRC).
33072135	1	15	theme	colorectal	198:207	arg1	cancer					209:214	colorectal cancer	198:214	colorectal cancer (CRC)	198:220	Berberine (BBR) has been reported that it has effects on inhibiting colorectal cancer (CRC).
33072135	8	16	theme	AOM/DSS	1123:1129	arg1	mice					1131:1134	AOM/DSS mice	1123:1134	AOM/DSS mice	1123:1134	Moreover, BBR altered the composition of gut microbiota in AOM/DSS mice obviously, which were characterized by a decrease of Actinobacteria and Verrucomicrobia significantly at the phylum level.
33072135	6	17	theme	metabolic	861:869	arg1	alterations					871:881	the metabolic alterations	857:881	the metabolic alterations	857:881	Meanwhile, feces samples were analyzed with 1H NMR spectroscopy to investigate the metabolic alterations.
33072135	0	18	from	Mice	82:85	arg1	View					11:14	A Holistic View	0:14	A Holistic View of Berberine Inhibiting Intestinal Carcinogenesis in Conventional Mice	0:85	A Holistic View of Berberine Inhibiting Intestinal Carcinogenesis in Conventional Mice Based on Microbiome-Metabolomics Analysis.
33072135	10	19	theme	induced	1535:1541	arg1	changes					1553:1559	BBR induced metabolic changes	1531:1559	BBR induced metabolic changes in feces	1531:1568	Metabolic data further revealed that BBR induced metabolic changes in feces focus on regulating glycometabolism, SCFA metabolism and amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are the products of interactions between the host and the microbial community.
33072135	3	20	from	effects	330:336	arg1	CRC					348:350	CRC	348:350	CRC	348:350	Herein, we investigated the therapeutic effects of BBR on CRC from the perspective of gut microbiota and metabolic alterations, which can provide a holistic view to understand the effects of BBR on CRC.
33072135	10	21	theme	feces	1726:1730	arg1	metabolites					1732:1742	these feces metabolites	1720:1742	these feces metabolites	1720:1742	Metabolic data further revealed that BBR induced metabolic changes in feces focus on regulating glycometabolism, SCFA metabolism and amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are the products of interactions between the host and the microbial community.
33072135	10	21	theme	feces	1726:1730	arg1	products					1752:1759	the products	1748:1759	the products of interactions between the host and the microbial community	1748:1820	Metabolic data further revealed that BBR induced metabolic changes in feces focus on regulating glycometabolism, SCFA metabolism and amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are the products of interactions between the host and the microbial community.
33072135	7	22	theme	intestinal	923:932	arg1	development					940:950	intestinal tumor development	923:950	intestinal tumor development with lower macroscopic polyps	923:980	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	7	22	theme	intestinal	923:932	arg1	morphology					1044:1053	better colonic morphology	1029:1053	better colonic morphology in mice	1029:1061	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	7	22	theme	intestinal	923:932	arg1	result					889:894	a result	887:894	a result	887:894	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	7	22	theme	intestinal	923:932	arg1	expression					992:1001	ki-67 expression	986:1001	ki-67 expression of intestinal tissue	986:1022	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	9	23	theme	short-chain	1382:1392	arg1	SCFA					1407:1410	SCFA	1407:1410	SCFA	1407:1410	At the genus level, it was able to suppress pathogenic species, such as f_Erysipelotrichaceae, Alistipes, and elevate some short-chain fatty acids (SCFA)-producing bacteria, including Alloprevotella, Flavonifractor, and Oscillibacter.
33072135	9	23	theme	short-chain	1382:1392	arg1	acids					1400:1404	some short-chain fatty acids	1377:1404	some short-chain fatty acids (SCFA)-producing bacteria, including Alloprevotella, Flavonifractor, and Oscillibacter	1377:1491	At the genus level, it was able to suppress pathogenic species, such as f_Erysipelotrichaceae, Alistipes, and elevate some short-chain fatty acids (SCFA)-producing bacteria, including Alloprevotella, Flavonifractor, and Oscillibacter.
33072135	4	24	theme	carcinogenesis	614:627	arg1	degree					593:598	the degree	589:598	the degree of colorectal carcinogenesis in AOM/DSS mice with or without BBR administration	589:678	First, azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse was used as CRC animal model, then the degree of colorectal carcinogenesis in AOM/DSS mice with or without BBR administration was measured.
33072135	7	25	theme	macroscopic	963:973	arg1	polyps					975:980	lower macroscopic polyps	957:980	lower macroscopic polyps	957:980	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	11	26	from	alterations	1858:1868	arg1	metabolic					1888:1896	metabolic	1888:1896	metabolic	1888:1896	This study showed that BBR induced alterations in microbiota and metabolic in AOM/DSS mice, which might providing new insight into the inhibition effects of BBR on CRC.
33072135	11	26	from	alterations	1858:1868	arg1	mice					1909:1912	AOM/DSS mice	1901:1912	AOM/DSS mice	1901:1912	This study showed that BBR induced alterations in microbiota and metabolic in AOM/DSS mice, which might providing new insight into the inhibition effects of BBR on CRC.
33072135	11	26	from	alterations	1858:1868	arg1	microbiota					1873:1882	microbiota	1873:1882	microbiota	1873:1882	This study showed that BBR induced alterations in microbiota and metabolic in AOM/DSS mice, which might providing new insight into the inhibition effects of BBR on CRC.
33072135	4	27	theme	AOM/DSS	632:638	arg1	mice					640:643	AOM/DSS mice	632:643	AOM/DSS mice with or without BBR administration	632:678	First, azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse was used as CRC animal model, then the degree of colorectal carcinogenesis in AOM/DSS mice with or without BBR administration was measured.
33072135	4	28	used	used	558:561	arg2	mouse					548:552	azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse	500:552	azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse	500:552	First, azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse was used as CRC animal model, then the degree of colorectal carcinogenesis in AOM/DSS mice with or without BBR administration was measured.
33072135	4	28	used	used	558:561	arg2	model					577:581	CRC animal model	566:581	CRC animal model	566:581	First, azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse was used as CRC animal model, then the degree of colorectal carcinogenesis in AOM/DSS mice with or without BBR administration was measured.
33072135	10	29	dep	host	1789:1792	arg1	the					1785:1787	the	1785:1787	the	1785:1787	Metabolic data further revealed that BBR induced metabolic changes in feces focus on regulating glycometabolism, SCFA metabolism and amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are the products of interactions between the host and the microbial community.
33072135	11	30	theme	AOM/DSS	1901:1907	arg1	mice					1909:1912	AOM/DSS mice	1901:1912	AOM/DSS mice	1901:1912	This study showed that BBR induced alterations in microbiota and metabolic in AOM/DSS mice, which might providing new insight into the inhibition effects of BBR on CRC.
33072135	0	31	from	View	11:14	arg1	Mice					82:85	Conventional Mice	69:85	Conventional Mice	69:85	A Holistic View of Berberine Inhibiting Intestinal Carcinogenesis in Conventional Mice Based on Microbiome-Metabolomics Analysis.
33072135	11	32	from	effects	1969:1975	arg1	CRC					1987:1989	CRC	1987:1989	CRC	1987:1989	This study showed that BBR induced alterations in microbiota and metabolic in AOM/DSS mice, which might providing new insight into the inhibition effects of BBR on CRC.
33072135	3	33	theme	metabolic	395:403	arg1	alterations					405:415	metabolic alterations	395:415	metabolic alterations	395:415	Herein, we investigated the therapeutic effects of BBR on CRC from the perspective of gut microbiota and metabolic alterations, which can provide a holistic view to understand the effects of BBR on CRC.
33072135	7	34	from	expression	992:1001	arg1	mice					1058:1061	mice	1058:1061	mice	1058:1061	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	3	35	theme	microbiota	380:389	arg1	perspective					361:371	the perspective	357:371	the perspective of gut microbiota and metabolic alterations	357:415	Herein, we investigated the therapeutic effects of BBR on CRC from the perspective of gut microbiota and metabolic alterations, which can provide a holistic view to understand the effects of BBR on CRC.
33072135	3	36	theme	BBR	341:343	arg1	effects					330:336	the therapeutic effects	314:336	the therapeutic effects of BBR on CRC from the perspective of gut microbiota and metabolic alterations, which can provide a holistic view to understand the effects of BBR on CRC	314:490	Herein, we investigated the therapeutic effects of BBR on CRC from the perspective of gut microbiota and metabolic alterations, which can provide a holistic view to understand the effects of BBR on CRC.
33072135	5	37	theme	microbiota	731:740	arg1	composition					698:708	composition	698:708	composition	698:708	The composition and abundance of gut microbiota was investigated by using 16S rRNA.
33072135	5	37	theme	microbiota	731:740	arg1	abundance					714:722	abundance	714:722	abundance	714:722	The composition and abundance of gut microbiota was investigated by using 16S rRNA.
33072135	6	38	theme	Meanwhile	778:786	arg1	samples					795:801	Meanwhile, feces samples	778:801	Meanwhile, feces samples	778:801	Meanwhile, feces samples were analyzed with 1H NMR spectroscopy to investigate the metabolic alterations.
33072135	4	39	theme	azoxymethane	500:511	arg1	DSS					543:545	DSS	543:545	DSS	543:545	First, azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse was used as CRC animal model, then the degree of colorectal carcinogenesis in AOM/DSS mice with or without BBR administration was measured.
33072135	4	39	theme	azoxymethane	500:511	arg1	sulfate					534:540	azoxymethane (AOM)/dextran sodium sulfate	500:540	azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse	500:552	First, azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse was used as CRC animal model, then the degree of colorectal carcinogenesis in AOM/DSS mice with or without BBR administration was measured.
33072135	0	40	theme	Berberine	19:27	arg1	Carcinogenesis					51:64	Berberine Inhibiting Intestinal Carcinogenesis	19:64	Berberine Inhibiting Intestinal Carcinogenesis in Conventional Mice	19:85	A Holistic View of Berberine Inhibiting Intestinal Carcinogenesis in Conventional Mice Based on Microbiome-Metabolomics Analysis.
33072135	7	41	with	expression	992:1001	arg1	polyps					975:980	lower macroscopic polyps	957:980	lower macroscopic polyps	957:980	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	10	42	theme	interactions	1764:1775	arg1	metabolites					1732:1742	these feces metabolites	1720:1742	these feces metabolites	1720:1742	Metabolic data further revealed that BBR induced metabolic changes in feces focus on regulating glycometabolism, SCFA metabolism and amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are the products of interactions between the host and the microbial community.
33072135	10	42	theme	interactions	1764:1775	arg1	products					1752:1759	the products	1748:1759	the products of interactions between the host and the microbial community	1748:1820	Metabolic data further revealed that BBR induced metabolic changes in feces focus on regulating glycometabolism, SCFA metabolism and amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are the products of interactions between the host and the microbial community.
33072135	10	43	theme	amino	1627:1631	arg1	metabolism					1638:1647	amino acid metabolism	1627:1647	amino acid metabolism	1627:1647	Metabolic data further revealed that BBR induced metabolic changes in feces focus on regulating glycometabolism, SCFA metabolism and amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are the products of interactions between the host and the microbial community.
33072135	0	44	theme	Intestinal	40:49	arg1	Carcinogenesis					51:64	Berberine Inhibiting Intestinal Carcinogenesis	19:64	Berberine Inhibiting Intestinal Carcinogenesis in Conventional Mice	19:85	A Holistic View of Berberine Inhibiting Intestinal Carcinogenesis in Conventional Mice Based on Microbiome-Metabolomics Analysis.
33072135	7	45	from	morphology	1044:1053	arg1	mice					1058:1061	mice	1058:1061	mice	1058:1061	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	10	46	theme	microbial	1802:1810	arg1	community					1812:1820	the microbial community	1798:1820	the microbial community	1798:1820	Metabolic data further revealed that BBR induced metabolic changes in feces focus on regulating glycometabolism, SCFA metabolism and amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are the products of interactions between the host and the microbial community.
33072135	4	47	theme	sulfate	534:540	arg1	mouse					548:552	azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse	500:552	azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse	500:552	First, azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse was used as CRC animal model, then the degree of colorectal carcinogenesis in AOM/DSS mice with or without BBR administration was measured.
33072135	4	47	theme	sulfate	534:540	arg1	model					577:581	CRC animal model	566:581	CRC animal model	566:581	First, azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse was used as CRC animal model, then the degree of colorectal carcinogenesis in AOM/DSS mice with or without BBR administration was measured.
33072135	6	48	theme	NMR	825:827	arg1	spectroscopy					829:840	1H NMR spectroscopy	822:840	1H NMR spectroscopy	822:840	Meanwhile, feces samples were analyzed with 1H NMR spectroscopy to investigate the metabolic alterations.
33072135	7	49	theme	colonic	1036:1042	arg1	development					940:950	intestinal tumor development	923:950	intestinal tumor development with lower macroscopic polyps	923:980	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	7	49	theme	colonic	1036:1042	arg1	expression					992:1001	ki-67 expression	986:1001	ki-67 expression of intestinal tissue	986:1022	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	7	49	theme	colonic	1036:1042	arg1	result					889:894	a result	887:894	a result	887:894	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	7	49	theme	colonic	1036:1042	arg1	morphology					1044:1053	better colonic morphology	1029:1053	better colonic morphology in mice	1029:1061	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	4	50	from	degree	593:598	arg1	mice					640:643	AOM/DSS mice	632:643	AOM/DSS mice with or without BBR administration	632:678	First, azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse was used as CRC animal model, then the degree of colorectal carcinogenesis in AOM/DSS mice with or without BBR administration was measured.
33072135	7	51	with	morphology	1044:1053	arg1	polyps					975:980	lower macroscopic polyps	957:980	lower macroscopic polyps	957:980	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	4	52	theme	/dextran	518:525	arg1	DSS					543:545	DSS	543:545	DSS	543:545	First, azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse was used as CRC animal model, then the degree of colorectal carcinogenesis in AOM/DSS mice with or without BBR administration was measured.
33072135	4	52	theme	/dextran	518:525	arg1	sulfate					534:540	azoxymethane (AOM)/dextran sodium sulfate	500:540	azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse	500:552	First, azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse was used as CRC animal model, then the degree of colorectal carcinogenesis in AOM/DSS mice with or without BBR administration was measured.
33072135	7	53	theme	tissue	1017:1022	arg1	development					940:950	intestinal tumor development	923:950	intestinal tumor development with lower macroscopic polyps	923:980	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	7	53	theme	tissue	1017:1022	arg1	expression					992:1001	ki-67 expression	986:1001	ki-67 expression of intestinal tissue	986:1022	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	7	53	theme	tissue	1017:1022	arg1	result					889:894	a result	887:894	a result	887:894	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	7	53	theme	tissue	1017:1022	arg1	morphology					1044:1053	better colonic morphology	1029:1053	better colonic morphology in mice	1029:1061	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	3	54	theme	BBR	481:483	arg1	effects					470:476	the effects	466:476	the effects of BBR on CRC	466:490	Herein, we investigated the therapeutic effects of BBR on CRC from the perspective of gut microbiota and metabolic alterations, which can provide a holistic view to understand the effects of BBR on CRC.
33072135	5	55	theme	16S	768:770	arg1	rRNA					772:775	16S rRNA	768:775	16S rRNA	768:775	The composition and abundance of gut microbiota was investigated by using 16S rRNA.
33072135	4	56	theme	CRC	566:568	arg1	mouse					548:552	azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse	500:552	azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse	500:552	First, azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse was used as CRC animal model, then the degree of colorectal carcinogenesis in AOM/DSS mice with or without BBR administration was measured.
33072135	4	56	theme	CRC	566:568	arg1	model					577:581	CRC animal model	566:581	CRC animal model	566:581	First, azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse was used as CRC animal model, then the degree of colorectal carcinogenesis in AOM/DSS mice with or without BBR administration was measured.
33072135	10	57	theme	microbiota	1701:1710	arg1	alteration					1683:1692	alteration	1683:1692	alteration of the microbiota	1683:1710	Metabolic data further revealed that BBR induced metabolic changes in feces focus on regulating glycometabolism, SCFA metabolism and amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are the products of interactions between the host and the microbial community.
33072135	7	58	theme	ki-67	986:990	arg1	development					940:950	intestinal tumor development	923:950	intestinal tumor development with lower macroscopic polyps	923:980	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	7	58	theme	ki-67	986:990	arg1	morphology					1044:1053	better colonic morphology	1029:1053	better colonic morphology in mice	1029:1061	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	7	58	theme	ki-67	986:990	arg1	result					889:894	a result	887:894	a result	887:894	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	7	58	theme	ki-67	986:990	arg1	expression					992:1001	ki-67 expression	986:1001	ki-67 expression of intestinal tissue	986:1022	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	8	59	theme	gut	1105:1107	arg1	microbiota					1109:1118	gut microbiota	1105:1118	gut microbiota	1105:1118	Moreover, BBR altered the composition of gut microbiota in AOM/DSS mice obviously, which were characterized by a decrease of Actinobacteria and Verrucomicrobia significantly at the phylum level.
33072135	10	60	theme	metabolic	1543:1551	arg1	changes					1553:1559	BBR induced metabolic changes	1531:1559	BBR induced metabolic changes in feces	1531:1568	Metabolic data further revealed that BBR induced metabolic changes in feces focus on regulating glycometabolism, SCFA metabolism and amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are the products of interactions between the host and the microbial community.
33072135	4	61	theme	colorectal	603:612	arg1	carcinogenesis					614:627	colorectal carcinogenesis	603:627	colorectal carcinogenesis	603:627	First, azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse was used as CRC animal model, then the degree of colorectal carcinogenesis in AOM/DSS mice with or without BBR administration was measured.
33072135	10	62	theme	BBR	1531:1533	arg1	changes					1553:1559	BBR induced metabolic changes	1531:1559	BBR induced metabolic changes in feces	1531:1568	Metabolic data further revealed that BBR induced metabolic changes in feces focus on regulating glycometabolism, SCFA metabolism and amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are the products of interactions between the host and the microbial community.
33072135	7	63	theme	lower	957:961	arg1	polyps					975:980	lower macroscopic polyps	957:980	lower macroscopic polyps	957:980	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	7	64	theme	tumor	934:938	arg1	development					940:950	intestinal tumor development	923:950	intestinal tumor development with lower macroscopic polyps	923:980	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	7	64	theme	tumor	934:938	arg1	morphology					1044:1053	better colonic morphology	1029:1053	better colonic morphology in mice	1029:1061	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	7	64	theme	tumor	934:938	arg1	result					889:894	a result	887:894	a result	887:894	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	7	64	theme	tumor	934:938	arg1	expression					992:1001	ki-67 expression	986:1001	ki-67 expression of intestinal tissue	986:1022	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	9	65	theme	fatty	1394:1398	arg1	SCFA					1407:1410	SCFA	1407:1410	SCFA	1407:1410	At the genus level, it was able to suppress pathogenic species, such as f_Erysipelotrichaceae, Alistipes, and elevate some short-chain fatty acids (SCFA)-producing bacteria, including Alloprevotella, Flavonifractor, and Oscillibacter.
33072135	9	65	theme	fatty	1394:1398	arg1	acids					1400:1404	some short-chain fatty acids	1377:1404	some short-chain fatty acids (SCFA)-producing bacteria, including Alloprevotella, Flavonifractor, and Oscillibacter	1377:1491	At the genus level, it was able to suppress pathogenic species, such as f_Erysipelotrichaceae, Alistipes, and elevate some short-chain fatty acids (SCFA)-producing bacteria, including Alloprevotella, Flavonifractor, and Oscillibacter.
33072135	8	66	theme	Actinobacteria	1189:1202	arg1	decrease					1177:1184	a decrease	1175:1184	a decrease of Actinobacteria and Verrucomicrobia	1175:1222	Moreover, BBR altered the composition of gut microbiota in AOM/DSS mice obviously, which were characterized by a decrease of Actinobacteria and Verrucomicrobia significantly at the phylum level.
33072135	8	67	theme	Verrucomicrobia	1208:1222	arg1	decrease					1177:1184	a decrease	1175:1184	a decrease of Actinobacteria and Verrucomicrobia	1175:1222	Moreover, BBR altered the composition of gut microbiota in AOM/DSS mice obviously, which were characterized by a decrease of Actinobacteria and Verrucomicrobia significantly at the phylum level.
33072135	0	68	theme	Conventional	69:80	arg1	Mice					82:85	Conventional Mice	69:85	Conventional Mice	69:85	A Holistic View of Berberine Inhibiting Intestinal Carcinogenesis in Conventional Mice Based on Microbiome-Metabolomics Analysis.
33072135	3	69	from	perspective	361:371	arg1	CRC					348:350	CRC	348:350	CRC	348:350	Herein, we investigated the therapeutic effects of BBR on CRC from the perspective of gut microbiota and metabolic alterations, which can provide a holistic view to understand the effects of BBR on CRC.
33072135	8	70	dep	altered	1078:1084	arg1	characterized					1158:1170	characterized	1158:1170	were characterized by a decrease of Actinobacteria and Verrucomicrobia significantly at the phylum level	1153:1256	Moreover, BBR altered the composition of gut microbiota in AOM/DSS mice obviously, which were characterized by a decrease of Actinobacteria and Verrucomicrobia significantly at the phylum level.
33072135	8	71	theme	phylum	1245:1250	arg1	level					1252:1256	the phylum level	1241:1256	the phylum level	1241:1256	Moreover, BBR altered the composition of gut microbiota in AOM/DSS mice obviously, which were characterized by a decrease of Actinobacteria and Verrucomicrobia significantly at the phylum level.
33072135	0	72	theme	Microbiome-Metabolomics	96:118	arg1	Analysis					120:127	Microbiome-Metabolomics Analysis	96:127	Microbiome-Metabolomics Analysis	96:127	A Holistic View of Berberine Inhibiting Intestinal Carcinogenesis in Conventional Mice Based on Microbiome-Metabolomics Analysis.
33072135	9	73	from	level	1272:1276	arg1	able					1286:1289	able	1286:1289	able	1286:1289	At the genus level, it was able to suppress pathogenic species, such as f_Erysipelotrichaceae, Alistipes, and elevate some short-chain fatty acids (SCFA)-producing bacteria, including Alloprevotella, Flavonifractor, and Oscillibacter.
33072135	0	74	from	Carcinogenesis	51:64	arg1	Mice					82:85	Conventional Mice	69:85	Conventional Mice	69:85	A Holistic View of Berberine Inhibiting Intestinal Carcinogenesis in Conventional Mice Based on Microbiome-Metabolomics Analysis.
33072135	4	75	theme	BBR	661:663	arg1	administration					665:678	BBR administration	661:678	BBR administration	661:678	First, azoxymethane (AOM)/dextran sodium sulfate (DSS) mouse was used as CRC animal model, then the degree of colorectal carcinogenesis in AOM/DSS mice with or without BBR administration was measured.
33072135	3	76	theme	holistic	438:445	arg1	view					447:450	a holistic view	436:450	a holistic view to understand the effects of BBR on CRC	436:490	Herein, we investigated the therapeutic effects of BBR on CRC from the perspective of gut microbiota and metabolic alterations, which can provide a holistic view to understand the effects of BBR on CRC.
33072135	7	77	with	development	940:950	arg1	polyps					975:980	lower macroscopic polyps	957:980	lower macroscopic polyps	957:980	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	3	78	theme	therapeutic	318:328	arg1	effects					330:336	the therapeutic effects	314:336	the therapeutic effects of BBR on CRC from the perspective of gut microbiota and metabolic alterations, which can provide a holistic view to understand the effects of BBR on CRC	314:490	Herein, we investigated the therapeutic effects of BBR on CRC from the perspective of gut microbiota and metabolic alterations, which can provide a holistic view to understand the effects of BBR on CRC.
33072135	3	79	theme	alterations	405:415	arg1	perspective					361:371	the perspective	357:371	the perspective of gut microbiota and metabolic alterations	357:415	Herein, we investigated the therapeutic effects of BBR on CRC from the perspective of gut microbiota and metabolic alterations, which can provide a holistic view to understand the effects of BBR on CRC.
33072135	0	80	theme	Holistic	2:9	arg1	View					11:14	A Holistic View	0:14	A Holistic View of Berberine Inhibiting Intestinal Carcinogenesis in Conventional Mice	0:85	A Holistic View of Berberine Inhibiting Intestinal Carcinogenesis in Conventional Mice Based on Microbiome-Metabolomics Analysis.
33072135	5	81	theme	gut	727:729	arg1	microbiota					731:740	gut microbiota	727:740	gut microbiota	727:740	The composition and abundance of gut microbiota was investigated by using 16S rRNA.
33072135	0	82	theme	Inhibiting	29:38	arg1	Carcinogenesis					51:64	Berberine Inhibiting Intestinal Carcinogenesis	19:64	Berberine Inhibiting Intestinal Carcinogenesis in Conventional Mice	19:85	A Holistic View of Berberine Inhibiting Intestinal Carcinogenesis in Conventional Mice Based on Microbiome-Metabolomics Analysis.
33072135	9	83	theme	genus	1266:1270	arg1	level					1272:1276	the genus level	1262:1276	the genus level	1262:1276	At the genus level, it was able to suppress pathogenic species, such as f_Erysipelotrichaceae, Alistipes, and elevate some short-chain fatty acids (SCFA)-producing bacteria, including Alloprevotella, Flavonifractor, and Oscillibacter.
33072135	2	84	from	mechanism	236:244	arg1	CRC					256:258	CRC	256:258	CRC	256:258	However, the mechanism of BBR on CRC also remains largely unknown.
33072135	3	85	theme	gut	376:378	arg1	microbiota					380:389	gut microbiota	376:389	gut microbiota	376:389	Herein, we investigated the therapeutic effects of BBR on CRC from the perspective of gut microbiota and metabolic alterations, which can provide a holistic view to understand the effects of BBR on CRC.
33072135	1	86	contain	has	172:174	arg1	it					169:170	it	169:170	it	169:170	Berberine (BBR) has been reported that it has effects on inhibiting colorectal cancer (CRC).
33072135	1	86	contain	has	172:174	arg2	effects					176:182	effects	176:182	effects	176:182	Berberine (BBR) has been reported that it has effects on inhibiting colorectal cancer (CRC).
33072135	0	87	theme	Carcinogenesis	51:64	arg1	View					11:14	A Holistic View	0:14	A Holistic View of Berberine Inhibiting Intestinal Carcinogenesis in Conventional Mice	0:85	A Holistic View of Berberine Inhibiting Intestinal Carcinogenesis in Conventional Mice Based on Microbiome-Metabolomics Analysis.
33072135	10	88	from	changes	1553:1559	arg1	feces					1564:1568	feces	1564:1568	feces	1564:1568	Metabolic data further revealed that BBR induced metabolic changes in feces focus on regulating glycometabolism, SCFA metabolism and amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are the products of interactions between the host and the microbial community.
33072135	7	89	from	development	940:950	arg1	mice					1058:1061	mice	1058:1061	mice	1058:1061	As a result, BBR significantly reduced intestinal tumor development with lower macroscopic polyps and ki-67 expression of intestinal tissue, and better colonic morphology in mice.
33072135	10	90	theme	SCFA	1607:1610	arg1	metabolism					1612:1621	SCFA metabolism	1607:1621	SCFA metabolism	1607:1621	Metabolic data further revealed that BBR induced metabolic changes in feces focus on regulating glycometabolism, SCFA metabolism and amino acid metabolism, which also provides evidence for alteration of the microbiota because these feces metabolites are the products of interactions between the host and the microbial community.
33072135	11	91	dep	induced	1850:1856	arg1	providing					1927:1935	providing	1927:1935	might providing new insight into the inhibition effects of BBR on CRC	1921:1989	This study showed that BBR induced alterations in microbiota and metabolic in AOM/DSS mice, which might providing new insight into the inhibition effects of BBR on CRC.
33072135	9	92	theme	pathogenic	1303:1312	arg1	Alistipes					1354:1362	Alistipes	1354:1362	Alistipes	1354:1362	At the genus level, it was able to suppress pathogenic species, such as f_Erysipelotrichaceae, Alistipes, and elevate some short-chain fatty acids (SCFA)-producing bacteria, including Alloprevotella, Flavonifractor, and Oscillibacter.
33072135	9	92	theme	pathogenic	1303:1312	arg1	species					1314:1320	pathogenic species	1303:1320	pathogenic species	1303:1320	At the genus level, it was able to suppress pathogenic species, such as f_Erysipelotrichaceae, Alistipes, and elevate some short-chain fatty acids (SCFA)-producing bacteria, including Alloprevotella, Flavonifractor, and Oscillibacter.
32985073	0	0	theme	chitosan/collagen	90:106	arg1	hydrogel					108:115	a thermosensitive chitosan/collagen hydrogel	72:115	a thermosensitive chitosan/collagen hydrogel	72:115	Effects of beta-tricalcium phosphate nanoparticles on the properties of a thermosensitive chitosan/collagen hydrogel and controlled release of quercetin.
32985073	4	1	theme	physiological	834:846	arg1	temperature					848:858	physiological temperature	834:858	physiological temperature	834:858	Notably, the incorporation of 1%-3% (w/v) bTCP in the hydrogels did not interfere with the gelation process and time of the hydrogels at physiological temperature and pH levels.
32985073	6	2	theme	bTCP	1019:1022	arg1	incorporation					999:1011	The incorporation	995:1011	The incorporation of 3% bTCP	995:1022	The incorporation of 3% bTCP increased the mechanical strength but decreased the swelling and degradation rates, pore size, permeability, and quercetin release rate of the hydrogels.
32985073	3	3	dep	%	597:597	arg1	w/v					600:602	w/v	600:602	w/v	600:602	Thereafter, the effects of 1%-3% (w/v) bTCP on properties of the bTCP-bGP-2:1 (w/w) chitosan/collagen hydrogels were investigated.
32985073	3	3	dep	%	597:597	arg1	%					594:594	%	594:594	%	594:594	Thereafter, the effects of 1%-3% (w/v) bTCP on properties of the bTCP-bGP-2:1 (w/w) chitosan/collagen hydrogels were investigated.
32985073	3	4	from	effects	582:588	arg1	properties					613:622	properties	613:622	properties of the bTCP-bGP-2:1 (w/w) chitosan/collagen hydrogels	613:676	Thereafter, the effects of 1%-3% (w/v) bTCP on properties of the bTCP-bGP-2:1 (w/w) chitosan/collagen hydrogels were investigated.
32985073	8	5	theme	bone	1412:1415	arg1	regeneration					1417:1428	bone regeneration	1412:1428	bone regeneration	1412:1428	A sustained quercetin release profile of the 3% bTCP-hydrogel further suggested the applicability of the hydrogel as a delivery vehicle of natural flavonoids for bone regeneration.
32985073	0	6	theme	thermosensitive	74:88	arg1	hydrogel					108:115	a thermosensitive chitosan/collagen hydrogel	72:115	a thermosensitive chitosan/collagen hydrogel	72:115	Effects of beta-tricalcium phosphate nanoparticles on the properties of a thermosensitive chitosan/collagen hydrogel and controlled release of quercetin.
32985073	6	7	theme	swelling	1076:1083	arg1	permeability					1119:1130	permeability	1119:1130	permeability	1119:1130	The incorporation of 3% bTCP increased the mechanical strength but decreased the swelling and degradation rates, pore size, permeability, and quercetin release rate of the hydrogels.
32985073	6	7	theme	swelling	1076:1083	arg1	rates					1101:1105	the swelling and degradation rates	1072:1105	the swelling and degradation rates	1072:1105	The incorporation of 3% bTCP increased the mechanical strength but decreased the swelling and degradation rates, pore size, permeability, and quercetin release rate of the hydrogels.
32985073	6	7	theme	swelling	1076:1083	arg1	size					1113:1116	pore size	1108:1116	pore size	1108:1116	The incorporation of 3% bTCP increased the mechanical strength but decreased the swelling and degradation rates, pore size, permeability, and quercetin release rate of the hydrogels.
32985073	6	7	theme	swelling	1076:1083	arg1	rate					1155:1158	quercetin release rate	1137:1158	quercetin release rate	1137:1158	The incorporation of 3% bTCP increased the mechanical strength but decreased the swelling and degradation rates, pore size, permeability, and quercetin release rate of the hydrogels.
32985073	4	8	dep	bTCP	739:742	arg1	%					731:731	%	731:731	%	731:731	Notably, the incorporation of 1%-3% (w/v) bTCP in the hydrogels did not interfere with the gelation process and time of the hydrogels at physiological temperature and pH levels.
32985073	0	9	from	Effects	0:6	arg1	properties					58:67	the properties	54:67	the properties of a thermosensitive chitosan/collagen hydrogel	54:115	Effects of beta-tricalcium phosphate nanoparticles on the properties of a thermosensitive chitosan/collagen hydrogel and controlled release of quercetin.
32985073	0	9	from	Effects	0:6	arg1	release					132:138	controlled release	121:138	controlled release of quercetin	121:151	Effects of beta-tricalcium phosphate nanoparticles on the properties of a thermosensitive chitosan/collagen hydrogel and controlled release of quercetin.
32985073	5	10	theme	pore	940:943	arg1	architecture					945:956	interconnecting pore architecture	924:956	interconnecting pore architecture	924:956	The bTCP-hydrogels exhibited a porous structure, interconnecting pore architecture, and median pore size of 100-200 μm.
32985073	0	11	theme	hydrogel	108:115	arg1	release					132:138	controlled release	121:138	controlled release of quercetin	121:151	Effects of beta-tricalcium phosphate nanoparticles on the properties of a thermosensitive chitosan/collagen hydrogel and controlled release of quercetin.
32985073	0	11	theme	hydrogel	108:115	arg1	properties					58:67	the properties	54:67	the properties of a thermosensitive chitosan/collagen hydrogel	54:115	Effects of beta-tricalcium phosphate nanoparticles on the properties of a thermosensitive chitosan/collagen hydrogel and controlled release of quercetin.
32985073	1	12	theme	bTCP-chitosan/collagen-quercetin	370:401	arg1	hydrogels					403:411	thermosensitive bTCP-chitosan/collagen-quercetin hydrogels	354:411	thermosensitive bTCP-chitosan/collagen-quercetin hydrogels	354:411	In the present study, an inorganic matrix of beta-tricalcium phosphate (bTCP) nanoparticles and quercetin was incorporated into an organic matrix of 2:1 (w/w) chitosan/collagen composite to fabricate thermosensitive bTCP-chitosan/collagen-quercetin hydrogels.
32985073	4	13	theme	bTCP	739:742	arg1	incorporation					710:722	the incorporation	706:722	the incorporation of 1%-3% (w/v) bTCP in the hydrogels	706:759	Notably, the incorporation of 1%-3% (w/v) bTCP in the hydrogels did not interfere with the gelation process and time of the hydrogels at physiological temperature and pH levels.
32985073	8	14	theme	hydrogel	1355:1362	arg1	vehicle					1378:1384	a delivery vehicle	1367:1384	a delivery vehicle of natural flavonoids for bone regeneration	1367:1428	A sustained quercetin release profile of the 3% bTCP-hydrogel further suggested the applicability of the hydrogel as a delivery vehicle of natural flavonoids for bone regeneration.
32985073	8	14	theme	hydrogel	1355:1362	arg1	applicability					1334:1346	the applicability	1330:1346	the applicability of the hydrogel	1330:1362	A sustained quercetin release profile of the 3% bTCP-hydrogel further suggested the applicability of the hydrogel as a delivery vehicle of natural flavonoids for bone regeneration.
32985073	3	15	theme	1	593:593	arg1	%					594:594	%	594:594	%	594:594	Thereafter, the effects of 1%-3% (w/v) bTCP on properties of the bTCP-bGP-2:1 (w/w) chitosan/collagen hydrogels were investigated.
32985073	5	16	theme	porous	906:911	arg1	structure					913:921	a porous structure	904:921	a porous structure	904:921	The bTCP-hydrogels exhibited a porous structure, interconnecting pore architecture, and median pore size of 100-200 μm.
32985073	6	17	theme	pore	1108:1111	arg1	rates					1101:1105	the swelling and degradation rates	1072:1105	the swelling and degradation rates	1072:1105	The incorporation of 3% bTCP increased the mechanical strength but decreased the swelling and degradation rates, pore size, permeability, and quercetin release rate of the hydrogels.
32985073	6	17	theme	pore	1108:1111	arg1	size					1113:1116	pore size	1108:1116	pore size	1108:1116	The incorporation of 3% bTCP increased the mechanical strength but decreased the swelling and degradation rates, pore size, permeability, and quercetin release rate of the hydrogels.
32985073	4	18	dep	process	797:803	arg1	the					784:786	the	784:786	the	784:786	Notably, the incorporation of 1%-3% (w/v) bTCP in the hydrogels did not interfere with the gelation process and time of the hydrogels at physiological temperature and pH levels.
32985073	6	19	theme	quercetin	1137:1145	arg1	rates					1101:1105	the swelling and degradation rates	1072:1105	the swelling and degradation rates	1072:1105	The incorporation of 3% bTCP increased the mechanical strength but decreased the swelling and degradation rates, pore size, permeability, and quercetin release rate of the hydrogels.
32985073	6	19	theme	quercetin	1137:1145	arg1	rate					1155:1158	quercetin release rate	1137:1158	quercetin release rate	1137:1158	The incorporation of 3% bTCP increased the mechanical strength but decreased the swelling and degradation rates, pore size, permeability, and quercetin release rate of the hydrogels.
32985073	6	20	theme	mechanical	1038:1047	arg1	strength					1049:1056	the mechanical strength	1034:1056	the mechanical strength	1034:1056	The incorporation of 3% bTCP increased the mechanical strength but decreased the swelling and degradation rates, pore size, permeability, and quercetin release rate of the hydrogels.
32985073	6	21	theme	hydrogels	1167:1175	arg1	permeability					1119:1130	permeability	1119:1130	permeability	1119:1130	The incorporation of 3% bTCP increased the mechanical strength but decreased the swelling and degradation rates, pore size, permeability, and quercetin release rate of the hydrogels.
32985073	6	21	theme	hydrogels	1167:1175	arg1	rates					1101:1105	the swelling and degradation rates	1072:1105	the swelling and degradation rates	1072:1105	The incorporation of 3% bTCP increased the mechanical strength but decreased the swelling and degradation rates, pore size, permeability, and quercetin release rate of the hydrogels.
32985073	6	21	theme	hydrogels	1167:1175	arg1	size					1113:1116	pore size	1108:1116	pore size	1108:1116	The incorporation of 3% bTCP increased the mechanical strength but decreased the swelling and degradation rates, pore size, permeability, and quercetin release rate of the hydrogels.
32985073	6	21	theme	hydrogels	1167:1175	arg1	rate					1155:1158	quercetin release rate	1137:1158	quercetin release rate	1137:1158	The incorporation of 3% bTCP increased the mechanical strength but decreased the swelling and degradation rates, pore size, permeability, and quercetin release rate of the hydrogels.
32985073	0	22	theme	controlled	121:130	arg1	release					132:138	controlled release	121:138	controlled release of quercetin	121:151	Effects of beta-tricalcium phosphate nanoparticles on the properties of a thermosensitive chitosan/collagen hydrogel and controlled release of quercetin.
32985073	8	23	theme	quercetin	1262:1270	arg1	profile					1280:1286	A sustained quercetin release profile	1250:1286	A sustained quercetin release profile of the 3% bTCP-hydrogel	1250:1310	A sustained quercetin release profile of the 3% bTCP-hydrogel further suggested the applicability of the hydrogel as a delivery vehicle of natural flavonoids for bone regeneration.
32985073	5	24	theme	median	963:968	arg1	size					975:978	median pore size	963:978	median pore size	963:978	The bTCP-hydrogels exhibited a porous structure, interconnecting pore architecture, and median pore size of 100-200 μm.
32985073	1	25	theme	inorganic	179:187	arg1	matrix					189:194	an inorganic matrix	176:194	an inorganic matrix of beta-tricalcium phosphate (bTCP) nanoparticles	176:244	In the present study, an inorganic matrix of beta-tricalcium phosphate (bTCP) nanoparticles and quercetin was incorporated into an organic matrix of 2:1 (w/w) chitosan/collagen composite to fabricate thermosensitive bTCP-chitosan/collagen-quercetin hydrogels.
32985073	2	26	theme	physiological	525:537	arg1	temperature					539:549	physiological temperature	525:549	physiological temperature	525:549	A sol-gel transition of the hydrogels was stimulated by beta-glycerophosphate (bGP) and temperature changes at physiological temperature and pH levels.
32985073	4	27	theme	hydrogels	821:829	arg1	time					809:812	time	809:812	time	809:812	Notably, the incorporation of 1%-3% (w/v) bTCP in the hydrogels did not interfere with the gelation process and time of the hydrogels at physiological temperature and pH levels.
32985073	4	27	theme	hydrogels	821:829	arg1	process					797:803	gelation process	788:803	gelation process	788:803	Notably, the incorporation of 1%-3% (w/v) bTCP in the hydrogels did not interfere with the gelation process and time of the hydrogels at physiological temperature and pH levels.
32985073	8	28	theme	flavonoids	1397:1406	arg1	vehicle					1378:1384	a delivery vehicle	1367:1384	a delivery vehicle of natural flavonoids for bone regeneration	1367:1428	A sustained quercetin release profile of the 3% bTCP-hydrogel further suggested the applicability of the hydrogel as a delivery vehicle of natural flavonoids for bone regeneration.
32985073	8	28	theme	flavonoids	1397:1406	arg1	applicability					1334:1346	the applicability	1330:1346	the applicability of the hydrogel	1330:1362	A sustained quercetin release profile of the 3% bTCP-hydrogel further suggested the applicability of the hydrogel as a delivery vehicle of natural flavonoids for bone regeneration.
32985073	3	29	dep	bTCP	605:608	arg1	%					597:597	%	597:597	%	597:597	Thereafter, the effects of 1%-3% (w/v) bTCP on properties of the bTCP-bGP-2:1 (w/w) chitosan/collagen hydrogels were investigated.
32985073	1	30	theme	organic	285:291	arg1	matrix					293:298	an organic matrix	282:298	an organic matrix of 2:1 (w/w) chitosan/collagen composite to fabricate thermosensitive bTCP-chitosan/collagen-quercetin hydrogels	282:411	In the present study, an inorganic matrix of beta-tricalcium phosphate (bTCP) nanoparticles and quercetin was incorporated into an organic matrix of 2:1 (w/w) chitosan/collagen composite to fabricate thermosensitive bTCP-chitosan/collagen-quercetin hydrogels.
32985073	2	31	theme	sol-gel	416:422	arg1	transition					424:433	A sol-gel transition	414:433	A sol-gel transition of the hydrogels	414:450	A sol-gel transition of the hydrogels was stimulated by beta-glycerophosphate (bGP) and temperature changes at physiological temperature and pH levels.
32985073	0	32	theme	phosphate	27:35	arg1	nanoparticles					37:49	beta-tricalcium phosphate nanoparticles	11:49	beta-tricalcium phosphate nanoparticles	11:49	Effects of beta-tricalcium phosphate nanoparticles on the properties of a thermosensitive chitosan/collagen hydrogel and controlled release of quercetin.
32985073	0	33	theme	quercetin	143:151	arg1	release					132:138	controlled release	121:138	controlled release of quercetin	121:151	Effects of beta-tricalcium phosphate nanoparticles on the properties of a thermosensitive chitosan/collagen hydrogel and controlled release of quercetin.
32985073	0	33	theme	quercetin	143:151	arg1	properties					58:67	the properties	54:67	the properties of a thermosensitive chitosan/collagen hydrogel	54:115	Effects of beta-tricalcium phosphate nanoparticles on the properties of a thermosensitive chitosan/collagen hydrogel and controlled release of quercetin.
32985073	8	34	theme	%	1296:1296	arg1	bTCP-hydrogel					1298:1310	the 3% bTCP-hydrogel	1291:1310	the 3% bTCP-hydrogel	1291:1310	A sustained quercetin release profile of the 3% bTCP-hydrogel further suggested the applicability of the hydrogel as a delivery vehicle of natural flavonoids for bone regeneration.
32985073	5	35	theme	interconnecting	924:938	arg1	architecture					945:956	interconnecting pore architecture	924:956	interconnecting pore architecture	924:956	The bTCP-hydrogels exhibited a porous structure, interconnecting pore architecture, and median pore size of 100-200 μm.
32985073	4	36	theme	gelation	788:795	arg1	process					797:803	gelation process	788:803	gelation process	788:803	Notably, the incorporation of 1%-3% (w/v) bTCP in the hydrogels did not interfere with the gelation process and time of the hydrogels at physiological temperature and pH levels.
32985073	1	37	theme	beta-tricalcium	199:213	arg1	nanoparticles					232:244	beta-tricalcium phosphate (bTCP) nanoparticles	199:244	beta-tricalcium phosphate (bTCP) nanoparticles	199:244	In the present study, an inorganic matrix of beta-tricalcium phosphate (bTCP) nanoparticles and quercetin was incorporated into an organic matrix of 2:1 (w/w) chitosan/collagen composite to fabricate thermosensitive bTCP-chitosan/collagen-quercetin hydrogels.
32985073	0	38	theme	beta-tricalcium	11:25	arg1	nanoparticles					37:49	beta-tricalcium phosphate nanoparticles	11:49	beta-tricalcium phosphate nanoparticles	11:49	Effects of beta-tricalcium phosphate nanoparticles on the properties of a thermosensitive chitosan/collagen hydrogel and controlled release of quercetin.
32985073	3	39	theme	-3	595:596	arg1	%					594:594	%	594:594	%	594:594	Thereafter, the effects of 1%-3% (w/v) bTCP on properties of the bTCP-bGP-2:1 (w/w) chitosan/collagen hydrogels were investigated.
32985073	1	40	theme	phosphate	215:223	arg1	nanoparticles					232:244	beta-tricalcium phosphate (bTCP) nanoparticles	199:244	beta-tricalcium phosphate (bTCP) nanoparticles	199:244	In the present study, an inorganic matrix of beta-tricalcium phosphate (bTCP) nanoparticles and quercetin was incorporated into an organic matrix of 2:1 (w/w) chitosan/collagen composite to fabricate thermosensitive bTCP-chitosan/collagen-quercetin hydrogels.
32985073	1	41	theme	2:1	303:305	arg1	composite					331:339	2:1 (w/w) chitosan/collagen composite	303:339	2:1 (w/w) chitosan/collagen composite	303:339	In the present study, an inorganic matrix of beta-tricalcium phosphate (bTCP) nanoparticles and quercetin was incorporated into an organic matrix of 2:1 (w/w) chitosan/collagen composite to fabricate thermosensitive bTCP-chitosan/collagen-quercetin hydrogels.
32985073	3	42	theme	chitosan/collagen	650:666	arg1	hydrogels					668:676	the bTCP-bGP-2:1 (w/w) chitosan/collagen hydrogels	627:676	the bTCP-bGP-2:1 (w/w) chitosan/collagen hydrogels	627:676	Thereafter, the effects of 1%-3% (w/v) bTCP on properties of the bTCP-bGP-2:1 (w/w) chitosan/collagen hydrogels were investigated.
32985073	3	43	theme	bTCP	605:608	arg1	effects					582:588	the effects	578:588	the effects of 1%-3% (w/v) bTCP on properties of the bTCP-bGP-2:1 (w/w) chitosan/collagen hydrogels	578:676	Thereafter, the effects of 1%-3% (w/v) bTCP on properties of the bTCP-bGP-2:1 (w/w) chitosan/collagen hydrogels were investigated.
32985073	6	44	theme	%	1017:1017	arg1	bTCP					1019:1022	3% bTCP	1016:1022	3% bTCP	1016:1022	The incorporation of 3% bTCP increased the mechanical strength but decreased the swelling and degradation rates, pore size, permeability, and quercetin release rate of the hydrogels.
32985073	0	45	theme	nanoparticles	37:49	arg1	Effects					0:6	Effects	0:6	Effects of beta-tricalcium phosphate nanoparticles on the properties of a thermosensitive chitosan/collagen hydrogel and controlled release of quercetin.	0:152	Effects of beta-tricalcium phosphate nanoparticles on the properties of a thermosensitive chitosan/collagen hydrogel and controlled release of quercetin.
32985073	1	46	theme	bTCP	226:229	arg1	nanoparticles					232:244	beta-tricalcium phosphate (bTCP) nanoparticles	199:244	beta-tricalcium phosphate (bTCP) nanoparticles	199:244	In the present study, an inorganic matrix of beta-tricalcium phosphate (bTCP) nanoparticles and quercetin was incorporated into an organic matrix of 2:1 (w/w) chitosan/collagen composite to fabricate thermosensitive bTCP-chitosan/collagen-quercetin hydrogels.
32985073	2	47	theme	temperature	502:512	arg1	changes					514:520	temperature changes	502:520	temperature changes	502:520	A sol-gel transition of the hydrogels was stimulated by beta-glycerophosphate (bGP) and temperature changes at physiological temperature and pH levels.
32985073	1	48	theme	w/w	308:310	arg1	composite					331:339	2:1 (w/w) chitosan/collagen composite	303:339	2:1 (w/w) chitosan/collagen composite	303:339	In the present study, an inorganic matrix of beta-tricalcium phosphate (bTCP) nanoparticles and quercetin was incorporated into an organic matrix of 2:1 (w/w) chitosan/collagen composite to fabricate thermosensitive bTCP-chitosan/collagen-quercetin hydrogels.
32985073	6	49	theme	3	1016:1016	arg1	%					1017:1017	%	1017:1017	%	1017:1017	The incorporation of 3% bTCP increased the mechanical strength but decreased the swelling and degradation rates, pore size, permeability, and quercetin release rate of the hydrogels.
32985073	3	50	theme	w/w	645:647	arg1	hydrogels					668:676	the bTCP-bGP-2:1 (w/w) chitosan/collagen hydrogels	627:676	the bTCP-bGP-2:1 (w/w) chitosan/collagen hydrogels	627:676	Thereafter, the effects of 1%-3% (w/v) bTCP on properties of the bTCP-bGP-2:1 (w/w) chitosan/collagen hydrogels were investigated.
32985073	4	51	theme	1	727:727	arg1	%					728:728	%	728:728	%	728:728	Notably, the incorporation of 1%-3% (w/v) bTCP in the hydrogels did not interfere with the gelation process and time of the hydrogels at physiological temperature and pH levels.
32985073	1	52	theme	thermosensitive	354:368	arg1	hydrogels					403:411	thermosensitive bTCP-chitosan/collagen-quercetin hydrogels	354:411	thermosensitive bTCP-chitosan/collagen-quercetin hydrogels	354:411	In the present study, an inorganic matrix of beta-tricalcium phosphate (bTCP) nanoparticles and quercetin was incorporated into an organic matrix of 2:1 (w/w) chitosan/collagen composite to fabricate thermosensitive bTCP-chitosan/collagen-quercetin hydrogels.
32985073	6	53	theme	release	1147:1153	arg1	rates					1101:1105	the swelling and degradation rates	1072:1105	the swelling and degradation rates	1072:1105	The incorporation of 3% bTCP increased the mechanical strength but decreased the swelling and degradation rates, pore size, permeability, and quercetin release rate of the hydrogels.
32985073	6	53	theme	release	1147:1153	arg1	rate					1155:1158	quercetin release rate	1137:1158	quercetin release rate	1137:1158	The incorporation of 3% bTCP increased the mechanical strength but decreased the swelling and degradation rates, pore size, permeability, and quercetin release rate of the hydrogels.
32985073	4	54	theme	pH	864:865	arg1	levels					867:872	pH levels	864:872	pH levels	864:872	Notably, the incorporation of 1%-3% (w/v) bTCP in the hydrogels did not interfere with the gelation process and time of the hydrogels at physiological temperature and pH levels.
32985073	8	55	theme	3	1295:1295	arg1	%					1296:1296	%	1296:1296	%	1296:1296	A sustained quercetin release profile of the 3% bTCP-hydrogel further suggested the applicability of the hydrogel as a delivery vehicle of natural flavonoids for bone regeneration.
32985073	8	56	theme	delivery	1369:1376	arg1	vehicle					1378:1384	a delivery vehicle	1367:1384	a delivery vehicle of natural flavonoids for bone regeneration	1367:1428	A sustained quercetin release profile of the 3% bTCP-hydrogel further suggested the applicability of the hydrogel as a delivery vehicle of natural flavonoids for bone regeneration.
32985073	8	56	theme	delivery	1369:1376	arg1	applicability					1334:1346	the applicability	1330:1346	the applicability of the hydrogel	1330:1362	A sustained quercetin release profile of the 3% bTCP-hydrogel further suggested the applicability of the hydrogel as a delivery vehicle of natural flavonoids for bone regeneration.
32985073	8	57	theme	sustained	1252:1260	arg1	profile					1280:1286	A sustained quercetin release profile	1250:1286	A sustained quercetin release profile of the 3% bTCP-hydrogel	1250:1310	A sustained quercetin release profile of the 3% bTCP-hydrogel further suggested the applicability of the hydrogel as a delivery vehicle of natural flavonoids for bone regeneration.
32985073	1	58	theme	chitosan/collagen	313:329	arg1	composite					331:339	2:1 (w/w) chitosan/collagen composite	303:339	2:1 (w/w) chitosan/collagen composite	303:339	In the present study, an inorganic matrix of beta-tricalcium phosphate (bTCP) nanoparticles and quercetin was incorporated into an organic matrix of 2:1 (w/w) chitosan/collagen composite to fabricate thermosensitive bTCP-chitosan/collagen-quercetin hydrogels.
32985073	8	59	theme	natural	1389:1395	arg1	flavonoids					1397:1406	natural flavonoids	1389:1406	natural flavonoids	1389:1406	A sustained quercetin release profile of the 3% bTCP-hydrogel further suggested the applicability of the hydrogel as a delivery vehicle of natural flavonoids for bone regeneration.
32985073	5	60	theme	pore	970:973	arg1	size					975:978	median pore size	963:978	median pore size	963:978	The bTCP-hydrogels exhibited a porous structure, interconnecting pore architecture, and median pore size of 100-200 μm.
32985073	4	61	theme	-3	729:730	arg1	%					728:728	%	728:728	%	728:728	Notably, the incorporation of 1%-3% (w/v) bTCP in the hydrogels did not interfere with the gelation process and time of the hydrogels at physiological temperature and pH levels.
32985073	6	62	theme	degradation	1089:1099	arg1	permeability					1119:1130	permeability	1119:1130	permeability	1119:1130	The incorporation of 3% bTCP increased the mechanical strength but decreased the swelling and degradation rates, pore size, permeability, and quercetin release rate of the hydrogels.
32985073	6	62	theme	degradation	1089:1099	arg1	rates					1101:1105	the swelling and degradation rates	1072:1105	the swelling and degradation rates	1072:1105	The incorporation of 3% bTCP increased the mechanical strength but decreased the swelling and degradation rates, pore size, permeability, and quercetin release rate of the hydrogels.
32985073	6	62	theme	degradation	1089:1099	arg1	size					1113:1116	pore size	1108:1116	pore size	1108:1116	The incorporation of 3% bTCP increased the mechanical strength but decreased the swelling and degradation rates, pore size, permeability, and quercetin release rate of the hydrogels.
32985073	6	62	theme	degradation	1089:1099	arg1	rate					1155:1158	quercetin release rate	1137:1158	quercetin release rate	1137:1158	The incorporation of 3% bTCP increased the mechanical strength but decreased the swelling and degradation rates, pore size, permeability, and quercetin release rate of the hydrogels.
32985073	3	63	theme	hydrogels	668:676	arg1	properties					613:622	properties	613:622	properties of the bTCP-bGP-2:1 (w/w) chitosan/collagen hydrogels	613:676	Thereafter, the effects of 1%-3% (w/v) bTCP on properties of the bTCP-bGP-2:1 (w/w) chitosan/collagen hydrogels were investigated.
32985073	3	64	theme	bTCP-bGP-2:1	631:642	arg1	hydrogels					668:676	the bTCP-bGP-2:1 (w/w) chitosan/collagen hydrogels	627:676	the bTCP-bGP-2:1 (w/w) chitosan/collagen hydrogels	627:676	Thereafter, the effects of 1%-3% (w/v) bTCP on properties of the bTCP-bGP-2:1 (w/w) chitosan/collagen hydrogels were investigated.
32985073	4	65	dep	%	731:731	arg1	w/v					734:736	w/v	734:736	w/v	734:736	Notably, the incorporation of 1%-3% (w/v) bTCP in the hydrogels did not interfere with the gelation process and time of the hydrogels at physiological temperature and pH levels.
32985073	4	65	dep	%	731:731	arg1	%					728:728	%	728:728	%	728:728	Notably, the incorporation of 1%-3% (w/v) bTCP in the hydrogels did not interfere with the gelation process and time of the hydrogels at physiological temperature and pH levels.
32985073	8	66	theme	bTCP-hydrogel	1298:1310	arg1	profile					1280:1286	A sustained quercetin release profile	1250:1286	A sustained quercetin release profile of the 3% bTCP-hydrogel	1250:1310	A sustained quercetin release profile of the 3% bTCP-hydrogel further suggested the applicability of the hydrogel as a delivery vehicle of natural flavonoids for bone regeneration.
32985073	1	67	theme	nanoparticles	232:244	arg1	matrix					189:194	an inorganic matrix	176:194	an inorganic matrix of beta-tricalcium phosphate (bTCP) nanoparticles	176:244	In the present study, an inorganic matrix of beta-tricalcium phosphate (bTCP) nanoparticles and quercetin was incorporated into an organic matrix of 2:1 (w/w) chitosan/collagen composite to fabricate thermosensitive bTCP-chitosan/collagen-quercetin hydrogels.
32985073	1	67	theme	nanoparticles	232:244	arg1	quercetin					250:258	quercetin	250:258	quercetin	250:258	In the present study, an inorganic matrix of beta-tricalcium phosphate (bTCP) nanoparticles and quercetin was incorporated into an organic matrix of 2:1 (w/w) chitosan/collagen composite to fabricate thermosensitive bTCP-chitosan/collagen-quercetin hydrogels.
32985073	2	68	theme	pH	555:556	arg1	levels					558:563	pH levels	555:563	pH levels	555:563	A sol-gel transition of the hydrogels was stimulated by beta-glycerophosphate (bGP) and temperature changes at physiological temperature and pH levels.
32985073	2	69	theme	hydrogels	442:450	arg1	transition					424:433	A sol-gel transition	414:433	A sol-gel transition of the hydrogels	414:450	A sol-gel transition of the hydrogels was stimulated by beta-glycerophosphate (bGP) and temperature changes at physiological temperature and pH levels.
32985073	8	70	theme	release	1272:1278	arg1	profile					1280:1286	A sustained quercetin release profile	1250:1286	A sustained quercetin release profile of the 3% bTCP-hydrogel	1250:1310	A sustained quercetin release profile of the 3% bTCP-hydrogel further suggested the applicability of the hydrogel as a delivery vehicle of natural flavonoids for bone regeneration.
32985073	1	71	theme	composite	331:339	arg1	matrix					293:298	an organic matrix	282:298	an organic matrix of 2:1 (w/w) chitosan/collagen composite to fabricate thermosensitive bTCP-chitosan/collagen-quercetin hydrogels	282:411	In the present study, an inorganic matrix of beta-tricalcium phosphate (bTCP) nanoparticles and quercetin was incorporated into an organic matrix of 2:1 (w/w) chitosan/collagen composite to fabricate thermosensitive bTCP-chitosan/collagen-quercetin hydrogels.
32985073	5	72	theme	100-200 μm	983:992	arg1	structure					913:921	a porous structure	904:921	a porous structure	904:921	The bTCP-hydrogels exhibited a porous structure, interconnecting pore architecture, and median pore size of 100-200 μm.
32985073	5	72	theme	100-200 μm	983:992	arg1	size					975:978	median pore size	963:978	median pore size	963:978	The bTCP-hydrogels exhibited a porous structure, interconnecting pore architecture, and median pore size of 100-200 μm.
32985073	5	72	theme	100-200 μm	983:992	arg1	architecture					945:956	interconnecting pore architecture	924:956	interconnecting pore architecture	924:956	The bTCP-hydrogels exhibited a porous structure, interconnecting pore architecture, and median pore size of 100-200 μm.
32985073	7	73	theme	cell	1230:1233	arg1	encapsulation					1235:1247	cell encapsulation	1230:1247	cell encapsulation	1230:1247	The hydrogels were noncytotoxic and able to support cell encapsulation.
32985073	1	74	theme	present	161:167	arg1	study					169:173	the present study	157:173	the present study	157:173	In the present study, an inorganic matrix of beta-tricalcium phosphate (bTCP) nanoparticles and quercetin was incorporated into an organic matrix of 2:1 (w/w) chitosan/collagen composite to fabricate thermosensitive bTCP-chitosan/collagen-quercetin hydrogels.
32985073	4	75	from	incorporation	710:722	arg1	hydrogels					751:759	the hydrogels	747:759	the hydrogels	747:759	Notably, the incorporation of 1%-3% (w/v) bTCP in the hydrogels did not interfere with the gelation process and time of the hydrogels at physiological temperature and pH levels.
33076996	14	0	theme	large-scale	2196:2206	arg1	study					2208:2212	a large-scale study	2194:2212	a large-scale study due to our study limitations	2194:2241	CONCLUSIONS In this study, α-mannosidase treatment of urine did not significantly increase the LAM-test performance, however; this needs to be further evaluated in a large-scale study due to our study limitations.
33076996	1	1	from	tuberculosis	256:267	arg1	people					302:307	severe immune suppressed people	277:307	severe immune suppressed people living with HIV (PLWH)	277:330	BACKGROUND Improved point-of-care diagnostic tests for tuberculosis (TB) in severe immune suppressed people living with HIV (PLWH) are needed to decrease morbidity and mortality outcomes.
33076996	15	2	theme	high	2257:2260	arg1	rates					2262:2266	high rates	2257:2266	high rates of TB incidence and mortality	2257:2296	Importantly, high rates of TB incidence and mortality were found, and a positive LAM-test result predicted mortality in PLWH with TB clinical symptoms.
33076996	2	3	theme	care	570:573	arg1	clinics					575:581	primary care clinics	562:581	primary care clinics in Guatemala	562:594	The aim of the study is to evaluate the performance of the lipoarabinomannan antigen test (LAM-test) with and without α-mannosidase pre-treated urine in a cohort of PLWH in primary care clinics in Guatemala.
33076996	3	4	theme	TB	687:688	arg1	symptoms					690:697	TB symptoms	687:697	TB symptoms	687:697	We further determined TB incidence, and mortality rates and its risk factors in PLWH with TB symptoms.
33076996	1	5	theme	severe	277:282	arg1	people					302:307	severe immune suppressed people	277:307	severe immune suppressed people living with HIV (PLWH)	277:330	BACKGROUND Improved point-of-care diagnostic tests for tuberculosis (TB) in severe immune suppressed people living with HIV (PLWH) are needed to decrease morbidity and mortality outcomes.
33076996	14	6	theme	urine	2084:2088	arg1	treatment					2071:2079	α-mannosidase treatment	2057:2079	α-mannosidase treatment of urine	2057:2088	CONCLUSIONS In this study, α-mannosidase treatment of urine did not significantly increase the LAM-test performance, however; this needs to be further evaluated in a large-scale study due to our study limitations.
33076996	15	7	theme	positive	2316:2323	arg1	result					2334:2339	a positive LAM-test result	2314:2339	a positive LAM-test result	2314:2339	Importantly, high rates of TB incidence and mortality were found, and a positive LAM-test result predicted mortality in PLWH with TB clinical symptoms.
33076996	13	8	theme	significant	1946:1956	arg1	value					1960:1964	a significant p value	1944:1964	a significant p value of 0.044	1944:1973	Importantly, PLWH with a positive LAM-test result had an adjusted hazard ratio (aHR) of death of 1.98 (1.0-3.8) with a significant p value of 0.044 when compared to PLWH with a negative LAM-test result.
33076996	6	9	theme	accuracy	1106:1113	arg1	studies					1115:1121	the LAM-test diagnostic accuracy studies	1082:1121	the LAM-test diagnostic accuracy studies	1082:1121	A composite reference standard of either a positive Mycobacterium tuberculosis complex culture and/or GeneXpert® MTB/RIF (Xpert, Cepheid, Sunnyvale, CA, USA) results was used in the LAM-test diagnostic accuracy studies.
33076996	8	10	theme	overall	1212:1218	arg1	%					1259:1259	of 56.1%	1252:1259	of 56.1%	1252:1259	RESULTS The overall sensitivity of the LAM-test was of 56.1% with 95% CI of (43.3-68.3).
33076996	8	10	theme	overall	1212:1218	arg1	sensitivity					1220:1230	The overall sensitivity	1208:1230	The overall sensitivity of the LAM-test	1208:1246	RESULTS The overall sensitivity of the LAM-test was of 56.1% with 95% CI of (43.3-68.3).
33076996	9	11	theme	T	1374:1374	arg1	values					1381:1386	CD4 T cell values	1370:1386	CD4 T cell values	1370:1386	There were no differences in the LAM-test sensitivity neither by hospital nor by CD4 T cell values.
33076996	6	12	theme	LAM-test	1086:1093	arg1	accuracy					1106:1113	the LAM-test diagnostic accuracy	1082:1113	the LAM-test diagnostic accuracy studies	1082:1121	A composite reference standard of either a positive Mycobacterium tuberculosis complex culture and/or GeneXpert® MTB/RIF (Xpert, Cepheid, Sunnyvale, CA, USA) results was used in the LAM-test diagnostic accuracy studies.
33076996	5	13	theme	HIV	799:801	arg1	sites					803:807	2 HIV sites	797:807	2 HIV sites	797:807	Urine samples were collected at 2 HIV sites to test the sensitivity of the LAM-test in urine with and without α-mannosidase pre-treatment.
33076996	8	14	theme	LAM-test	1239:1246	arg1	%					1259:1259	of 56.1%	1252:1259	of 56.1%	1252:1259	RESULTS The overall sensitivity of the LAM-test was of 56.1% with 95% CI of (43.3-68.3).
33076996	8	14	theme	LAM-test	1239:1246	arg1	sensitivity					1220:1230	The overall sensitivity	1208:1230	The overall sensitivity of the LAM-test	1208:1246	RESULTS The overall sensitivity of the LAM-test was of 56.1% with 95% CI of (43.3-68.3).
33076996	13	15	theme	hazard	1893:1898	arg1	aHR					1907:1909	aHR	1907:1909	aHR	1907:1909	Importantly, PLWH with a positive LAM-test result had an adjusted hazard ratio (aHR) of death of 1.98 (1.0-3.8) with a significant p value of 0.044 when compared to PLWH with a negative LAM-test result.
33076996	13	15	theme	hazard	1893:1898	arg1	ratio					1900:1904	an adjusted hazard ratio	1881:1904	an adjusted hazard ratio (aHR) of death of 1.98 (1.0-3.8) with a significant p value of 0.044	1881:1973	Importantly, PLWH with a positive LAM-test result had an adjusted hazard ratio (aHR) of death of 1.98 (1.0-3.8) with a significant p value of 0.044 when compared to PLWH with a negative LAM-test result.
33076996	1	16	theme	diagnostic	235:244	arg1	tests					246:250	BACKGROUND Improved point-of-care diagnostic tests	201:250	BACKGROUND Improved point-of-care diagnostic tests for tuberculosis (TB) in severe immune suppressed people living with HIV (PLWH)	201:330	BACKGROUND Improved point-of-care diagnostic tests for tuberculosis (TB) in severe immune suppressed people living with HIV (PLWH) are needed to decrease morbidity and mortality outcomes.
33076996	12	17	from	incidence	1690:1698	arg1	cohort					1707:1712	our cohort	1703:1712	our cohort	1703:1712	TB incidence in our cohort was of 21.4/100 person years (PYs) (95% CI 16.6-27.6), and mortality rate was of 11.1/100 PYs (95% CI 8.2-15.0).
33076996	13	18	theme	p	1958:1958	arg1	value					1960:1964	a significant p value	1944:1964	a significant p value of 0.044	1944:1973	Importantly, PLWH with a positive LAM-test result had an adjusted hazard ratio (aHR) of death of 1.98 (1.0-3.8) with a significant p value of 0.044 when compared to PLWH with a negative LAM-test result.
33076996	15	19	with	PLWH	2364:2367	arg1	symptoms					2386:2393	TB clinical symptoms	2374:2393	TB clinical symptoms	2374:2393	Importantly, high rates of TB incidence and mortality were found, and a positive LAM-test result predicted mortality in PLWH with TB clinical symptoms.
33076996	13	20	theme	positive	1852:1859	arg1	result					1870:1875	a positive LAM-test result	1850:1875	a positive LAM-test result	1850:1875	Importantly, PLWH with a positive LAM-test result had an adjusted hazard ratio (aHR) of death of 1.98 (1.0-3.8) with a significant p value of 0.044 when compared to PLWH with a negative LAM-test result.
33076996	5	21	from	sensitivity	821:831	arg1	urine					852:856	urine	852:856	urine	852:856	Urine samples were collected at 2 HIV sites to test the sensitivity of the LAM-test in urine with and without α-mannosidase pre-treatment.
33076996	5	22	theme	α-mannosidase	875:887	arg1	pre-treatment					889:901	α-mannosidase pre-treatment	875:901	α-mannosidase pre-treatment	875:901	Urine samples were collected at 2 HIV sites to test the sensitivity of the LAM-test in urine with and without α-mannosidase pre-treatment.
33076996	10	23	from	sensitivity	1398:1408	arg1	cells/µl					1435:1442	PLWH with < 200 CD4 T cells/µl	1413:1442	PLWH with < 200 CD4 T cells/µl	1413:1442	LAM-test sensitivity in PLWH with < 200 CD4 T cells/µl was of 62.2% (95% CI 46.5-76.2).
33076996	8	24	theme	95	1266:1267	arg1	%					1268:1268	%	1268:1268	%	1268:1268	RESULTS The overall sensitivity of the LAM-test was of 56.1% with 95% CI of (43.3-68.3).
33076996	11	25	theme	%	1628:1628	arg1	%					1623:1623	treated urine [55.2%	1604:1623	untreated vs. α-mannosidase treated urine [55.2% (95% CI 42.6-67.4)	1576:1642	There were no significant differences in sensitivity when comparing LAM-test results obtained from untreated vs. α-mannosidase treated urine [55.2% (95% CI 42.6-67.4) vs. 56.9% (95% CI 44-69.2), respectively].
33076996	11	25	theme	%	1628:1628	arg1	CI					1630:1631	95% CI 42.6-67.4	1626:1641	95% CI 42.6-67.4	1626:1641	There were no significant differences in sensitivity when comparing LAM-test results obtained from untreated vs. α-mannosidase treated urine [55.2% (95% CI 42.6-67.4) vs. 56.9% (95% CI 44-69.2), respectively].
33076996	0	26	theme	antigen	77:83	arg1	test					96:99	lipoarabinomannan antigen diagnostic test	59:99	lipoarabinomannan antigen diagnostic test using α-mannosidase treated and untreated urine in a cohort of people living with HIV in Guatemala	59:198	Accuracy of the tuberculosis point-of-care Alere determine lipoarabinomannan antigen diagnostic test using α-mannosidase treated and untreated urine in a cohort of people living with HIV in Guatemala.
33076996	6	27	used	used	1074:1077	arg2	reference					916:924	A composite reference standard	904:933	A composite reference standard of either a positive Mycobacterium tuberculosis complex culture and/or GeneXpert® MTB/RIF (Xpert, Cepheid, Sunnyvale, CA, USA) results	904:1068	A composite reference standard of either a positive Mycobacterium tuberculosis complex culture and/or GeneXpert® MTB/RIF (Xpert, Cepheid, Sunnyvale, CA, USA) results was used in the LAM-test diagnostic accuracy studies.
33076996	5	28	theme	LAM-test	840:847	arg1	sensitivity					821:831	the sensitivity	817:831	the sensitivity of the LAM-test in urine with and without α-mannosidase pre-treatment	817:901	Urine samples were collected at 2 HIV sites to test the sensitivity of the LAM-test in urine with and without α-mannosidase pre-treatment.
33076996	6	29	theme	complex	983:989	arg1	culture					991:997	a positive Mycobacterium tuberculosis complex culture	945:997	a positive Mycobacterium tuberculosis complex culture	945:997	A composite reference standard of either a positive Mycobacterium tuberculosis complex culture and/or GeneXpert® MTB/RIF (Xpert, Cepheid, Sunnyvale, CA, USA) results was used in the LAM-test diagnostic accuracy studies.
33076996	15	30	theme	clinical	2377:2384	arg1	symptoms					2386:2393	TB clinical symptoms	2374:2393	TB clinical symptoms	2374:2393	Importantly, high rates of TB incidence and mortality were found, and a positive LAM-test result predicted mortality in PLWH with TB clinical symptoms.
33076996	6	31	theme	Mycobacterium	956:968	arg1	culture					991:997	a positive Mycobacterium tuberculosis complex culture	945:997	a positive Mycobacterium tuberculosis complex culture	945:997	A composite reference standard of either a positive Mycobacterium tuberculosis complex culture and/or GeneXpert® MTB/RIF (Xpert, Cepheid, Sunnyvale, CA, USA) results was used in the LAM-test diagnostic accuracy studies.
33076996	2	32	theme	α-mannosidase	507:519	arg1	urine					533:537	α-mannosidase pre-treated urine	507:537	α-mannosidase pre-treated urine in a cohort of PLWH in primary care clinics in Guatemala	507:594	The aim of the study is to evaluate the performance of the lipoarabinomannan antigen test (LAM-test) with and without α-mannosidase pre-treated urine in a cohort of PLWH in primary care clinics in Guatemala.
33076996	0	33	theme	α-mannosidase	107:119	arg1	urine					143:147	α-mannosidase treated and untreated urine	107:147	α-mannosidase treated and untreated urine	107:147	Accuracy of the tuberculosis point-of-care Alere determine lipoarabinomannan antigen diagnostic test using α-mannosidase treated and untreated urine in a cohort of people living with HIV in Guatemala.
33076996	13	34	theme	negative	2004:2011	arg1	result					2022:2027	a negative LAM-test result	2002:2027	a negative LAM-test result	2002:2027	Importantly, PLWH with a positive LAM-test result had an adjusted hazard ratio (aHR) of death of 1.98 (1.0-3.8) with a significant p value of 0.044 when compared to PLWH with a negative LAM-test result.
33076996	10	35	theme	LAM-test	1389:1396	arg1	sensitivity					1398:1408	LAM-test sensitivity	1389:1408	LAM-test sensitivity in PLWH with < 200 CD4 T cells/µl	1389:1442	LAM-test sensitivity in PLWH with < 200 CD4 T cells/µl was of 62.2% (95% CI 46.5-76.2).
33076996	11	36	theme	%	1657:1657	arg1	CI					1659:1660	95% CI 44-69.2	1655:1668	95% CI 44-69.2	1655:1668	There were no significant differences in sensitivity when comparing LAM-test results obtained from untreated vs. α-mannosidase treated urine [55.2% (95% CI 42.6-67.4) vs. 56.9% (95% CI 44-69.2), respectively].
33076996	11	36	theme	%	1657:1657	arg1	%					1652:1652	56.9%	1648:1652	56.9% (95% CI 44-69.2)	1648:1669	There were no significant differences in sensitivity when comparing LAM-test results obtained from untreated vs. α-mannosidase treated urine [55.2% (95% CI 42.6-67.4) vs. 56.9% (95% CI 44-69.2), respectively].
33076996	2	37	from	clinics	575:581	arg1	Guatemala					586:594	Guatemala	586:594	Guatemala	586:594	The aim of the study is to evaluate the performance of the lipoarabinomannan antigen test (LAM-test) with and without α-mannosidase pre-treated urine in a cohort of PLWH in primary care clinics in Guatemala.
33076996	2	37	from	clinics	575:581	arg1	cohort					544:549	a cohort	542:549	a cohort of PLWH in primary care clinics in Guatemala	542:594	The aim of the study is to evaluate the performance of the lipoarabinomannan antigen test (LAM-test) with and without α-mannosidase pre-treated urine in a cohort of PLWH in primary care clinics in Guatemala.
33076996	0	38	theme	diagnostic	85:94	arg1	test					96:99	lipoarabinomannan antigen diagnostic test	59:99	lipoarabinomannan antigen diagnostic test using α-mannosidase treated and untreated urine in a cohort of people living with HIV in Guatemala	59:198	Accuracy of the tuberculosis point-of-care Alere determine lipoarabinomannan antigen diagnostic test using α-mannosidase treated and untreated urine in a cohort of people living with HIV in Guatemala.
33076996	7	39	theme	proportional	1128:1139	arg1	regression					1149:1158	Cox proportional hazards regression	1124:1158	Cox proportional hazards regression	1124:1158	Cox proportional hazards regression was used to study mortality predictors.
33076996	1	40	theme	immune	284:289	arg1	people					302:307	severe immune suppressed people	277:307	severe immune suppressed people living with HIV (PLWH)	277:330	BACKGROUND Improved point-of-care diagnostic tests for tuberculosis (TB) in severe immune suppressed people living with HIV (PLWH) are needed to decrease morbidity and mortality outcomes.
33076996	13	41	contain	had	1877:1879	arg2	ratio					1900:1904	an adjusted hazard ratio	1881:1904	an adjusted hazard ratio (aHR) of death of 1.98 (1.0-3.8) with a significant p value of 0.044	1881:1973	Importantly, PLWH with a positive LAM-test result had an adjusted hazard ratio (aHR) of death of 1.98 (1.0-3.8) with a significant p value of 0.044 when compared to PLWH with a negative LAM-test result.
33076996	13	41	contain	had	1877:1879	arg1	PLWH					1840:1843	PLWH	1840:1843	PLWH with a positive LAM-test result	1840:1875	Importantly, PLWH with a positive LAM-test result had an adjusted hazard ratio (aHR) of death of 1.98 (1.0-3.8) with a significant p value of 0.044 when compared to PLWH with a negative LAM-test result.
33076996	13	41	contain	had	1877:1879	arg2	aHR					1907:1909	aHR	1907:1909	aHR	1907:1909	Importantly, PLWH with a positive LAM-test result had an adjusted hazard ratio (aHR) of death of 1.98 (1.0-3.8) with a significant p value of 0.044 when compared to PLWH with a negative LAM-test result.
33076996	4	42	theme	longitudinal	720:731	arg1	study					733:737	Prospective longitudinal study	708:737	METHODS Prospective longitudinal study of PLWH with TB symptoms	700:762	METHODS Prospective longitudinal study of PLWH with TB symptoms.
33076996	2	43	theme	antigen	466:472	arg1	LAM-test					480:487	LAM-test	480:487	LAM-test	480:487	The aim of the study is to evaluate the performance of the lipoarabinomannan antigen test (LAM-test) with and without α-mannosidase pre-treated urine in a cohort of PLWH in primary care clinics in Guatemala.
33076996	2	43	theme	antigen	466:472	arg1	test					474:477	the lipoarabinomannan antigen test	444:477	the lipoarabinomannan antigen test (LAM-test)	444:488	The aim of the study is to evaluate the performance of the lipoarabinomannan antigen test (LAM-test) with and without α-mannosidase pre-treated urine in a cohort of PLWH in primary care clinics in Guatemala.
33076996	0	44	from	HIV	183:185	arg1	Guatemala					190:198	Guatemala	190:198	Guatemala	190:198	Accuracy of the tuberculosis point-of-care Alere determine lipoarabinomannan antigen diagnostic test using α-mannosidase treated and untreated urine in a cohort of people living with HIV in Guatemala.
33076996	11	45	theme	urine	1612:1616	arg1	%					1623:1623	treated urine [55.2%	1604:1623	untreated vs. α-mannosidase treated urine [55.2% (95% CI 42.6-67.4)	1576:1642	There were no significant differences in sensitivity when comparing LAM-test results obtained from untreated vs. α-mannosidase treated urine [55.2% (95% CI 42.6-67.4) vs. 56.9% (95% CI 44-69.2), respectively].
33076996	11	45	theme	urine	1612:1616	arg1	CI					1630:1631	95% CI 42.6-67.4	1626:1641	95% CI 42.6-67.4	1626:1641	There were no significant differences in sensitivity when comparing LAM-test results obtained from untreated vs. α-mannosidase treated urine [55.2% (95% CI 42.6-67.4) vs. 56.9% (95% CI 44-69.2), respectively].
33076996	0	46	theme	point-of-care	29:41	arg1	Alere					43:47	the tuberculosis point-of-care Alere	12:47	the tuberculosis point-of-care Alere	12:47	Accuracy of the tuberculosis point-of-care Alere determine lipoarabinomannan antigen diagnostic test using α-mannosidase treated and untreated urine in a cohort of people living with HIV in Guatemala.
33076996	6	47	theme	MTB/RIF	1017:1023	arg1	reference					916:924	A composite reference standard	904:933	A composite reference standard of either a positive Mycobacterium tuberculosis complex culture and/or GeneXpert® MTB/RIF (Xpert, Cepheid, Sunnyvale, CA, USA) results	904:1068	A composite reference standard of either a positive Mycobacterium tuberculosis complex culture and/or GeneXpert® MTB/RIF (Xpert, Cepheid, Sunnyvale, CA, USA) results was used in the LAM-test diagnostic accuracy studies.
33076996	11	48	theme	treated	1604:1610	arg1	%					1623:1623	treated urine [55.2%	1604:1623	untreated vs. α-mannosidase treated urine [55.2% (95% CI 42.6-67.4)	1576:1642	There were no significant differences in sensitivity when comparing LAM-test results obtained from untreated vs. α-mannosidase treated urine [55.2% (95% CI 42.6-67.4) vs. 56.9% (95% CI 44-69.2), respectively].
33076996	11	48	theme	treated	1604:1610	arg1	CI					1630:1631	95% CI 42.6-67.4	1626:1641	95% CI 42.6-67.4	1626:1641	There were no significant differences in sensitivity when comparing LAM-test results obtained from untreated vs. α-mannosidase treated urine [55.2% (95% CI 42.6-67.4) vs. 56.9% (95% CI 44-69.2), respectively].
33076996	3	49	theme	TB	619:620	arg1	incidence					622:630	TB incidence	619:630	TB incidence	619:630	We further determined TB incidence, and mortality rates and its risk factors in PLWH with TB symptoms.
33076996	5	50	theme	Urine	765:769	arg1	samples					771:777	Urine samples	765:777	Urine samples	765:777	Urine samples were collected at 2 HIV sites to test the sensitivity of the LAM-test in urine with and without α-mannosidase pre-treatment.
33076996	14	51	theme	study	2225:2229	arg1	limitations					2231:2241	our study limitations	2221:2241	our study limitations	2221:2241	CONCLUSIONS In this study, α-mannosidase treatment of urine did not significantly increase the LAM-test performance, however; this needs to be further evaluated in a large-scale study due to our study limitations.
33076996	10	52	theme	T	1433:1433	arg1	cells/µl					1435:1442	PLWH with < 200 CD4 T cells/µl	1413:1442	PLWH with < 200 CD4 T cells/µl	1413:1442	LAM-test sensitivity in PLWH with < 200 CD4 T cells/µl was of 62.2% (95% CI 46.5-76.2).
33076996	13	53	theme	death	1915:1919	arg1	aHR					1907:1909	aHR	1907:1909	aHR	1907:1909	Importantly, PLWH with a positive LAM-test result had an adjusted hazard ratio (aHR) of death of 1.98 (1.0-3.8) with a significant p value of 0.044 when compared to PLWH with a negative LAM-test result.
33076996	13	53	theme	death	1915:1919	arg1	ratio					1900:1904	an adjusted hazard ratio	1881:1904	an adjusted hazard ratio (aHR) of death of 1.98 (1.0-3.8) with a significant p value of 0.044	1881:1973	Importantly, PLWH with a positive LAM-test result had an adjusted hazard ratio (aHR) of death of 1.98 (1.0-3.8) with a significant p value of 0.044 when compared to PLWH with a negative LAM-test result.
33076996	14	54	dep	CONCLUSIONS	2030:2040	arg1	increase					2112:2119	increase	2112:2119	increase the LAM-test performance	2112:2144	CONCLUSIONS In this study, α-mannosidase treatment of urine did not significantly increase the LAM-test performance, however; this needs to be further evaluated in a large-scale study due to our study limitations.
33076996	14	54	dep	CONCLUSIONS	2030:2040	arg1	needs					2161:2165	needs	2161:2165	needs to be further evaluated in a large-scale study due to our study limitations	2161:2241	CONCLUSIONS In this study, α-mannosidase treatment of urine did not significantly increase the LAM-test performance, however; this needs to be further evaluated in a large-scale study due to our study limitations.
33076996	3	55	theme	mortality	637:645	arg1	rates					647:651	mortality rates	637:651	mortality rates	637:651	We further determined TB incidence, and mortality rates and its risk factors in PLWH with TB symptoms.
33076996	13	56	theme	1.98	1924:1927	arg1	aHR					1907:1909	aHR	1907:1909	aHR	1907:1909	Importantly, PLWH with a positive LAM-test result had an adjusted hazard ratio (aHR) of death of 1.98 (1.0-3.8) with a significant p value of 0.044 when compared to PLWH with a negative LAM-test result.
33076996	13	56	theme	1.98	1924:1927	arg1	ratio					1900:1904	an adjusted hazard ratio	1881:1904	an adjusted hazard ratio (aHR) of death of 1.98 (1.0-3.8) with a significant p value of 0.044	1881:1973	Importantly, PLWH with a positive LAM-test result had an adjusted hazard ratio (aHR) of death of 1.98 (1.0-3.8) with a significant p value of 0.044 when compared to PLWH with a negative LAM-test result.
33076996	11	57	theme	LAM-test	1545:1552	arg1	results					1554:1560	LAM-test results	1545:1560	LAM-test results obtained from untreated vs. α-mannosidase treated urine [55.2% (95% CI 42.6-67.4) vs. 56.9% (95% CI 44-69.2), respectively]	1545:1684	There were no significant differences in sensitivity when comparing LAM-test results obtained from untreated vs. α-mannosidase treated urine [55.2% (95% CI 42.6-67.4) vs. 56.9% (95% CI 44-69.2), respectively].
33076996	11	58	theme	significant	1491:1501	arg1	differences					1503:1513	no significant differences	1488:1513	no significant differences in sensitivity	1488:1528	There were no significant differences in sensitivity when comparing LAM-test results obtained from untreated vs. α-mannosidase treated urine [55.2% (95% CI 42.6-67.4) vs. 56.9% (95% CI 44-69.2), respectively].
33076996	15	59	theme	TB	2271:2272	arg1	incidence					2274:2282	TB incidence	2271:2282	TB incidence	2271:2282	Importantly, high rates of TB incidence and mortality were found, and a positive LAM-test result predicted mortality in PLWH with TB clinical symptoms.
33076996	12	60	theme	%	1811:1811	arg1	PYs					1804:1806	11.1/100 PYs	1795:1806	11.1/100 PYs (95% CI 8.2-15.0)	1795:1824	TB incidence in our cohort was of 21.4/100 person years (PYs) (95% CI 16.6-27.6), and mortality rate was of 11.1/100 PYs (95% CI 8.2-15.0).
33076996	12	60	theme	%	1811:1811	arg1	CI					1813:1814	95% CI 8.2-15.0	1809:1823	95% CI 8.2-15.0	1809:1823	TB incidence in our cohort was of 21.4/100 person years (PYs) (95% CI 16.6-27.6), and mortality rate was of 11.1/100 PYs (95% CI 8.2-15.0).
33076996	11	61	theme	α-mannosidase	1590:1602	arg1	%					1623:1623	treated urine [55.2%	1604:1623	untreated vs. α-mannosidase treated urine [55.2% (95% CI 42.6-67.4)	1576:1642	There were no significant differences in sensitivity when comparing LAM-test results obtained from untreated vs. α-mannosidase treated urine [55.2% (95% CI 42.6-67.4) vs. 56.9% (95% CI 44-69.2), respectively].
33076996	11	61	theme	α-mannosidase	1590:1602	arg1	CI					1630:1631	95% CI 42.6-67.4	1626:1641	95% CI 42.6-67.4	1626:1641	There were no significant differences in sensitivity when comparing LAM-test results obtained from untreated vs. α-mannosidase treated urine [55.2% (95% CI 42.6-67.4) vs. 56.9% (95% CI 44-69.2), respectively].
33076996	2	62	from	cohort	544:549	arg1	clinics					575:581	primary care clinics	562:581	primary care clinics in Guatemala	562:594	The aim of the study is to evaluate the performance of the lipoarabinomannan antigen test (LAM-test) with and without α-mannosidase pre-treated urine in a cohort of PLWH in primary care clinics in Guatemala.
33076996	1	63	theme	Improved	212:219	arg1	tests					246:250	BACKGROUND Improved point-of-care diagnostic tests	201:250	BACKGROUND Improved point-of-care diagnostic tests for tuberculosis (TB) in severe immune suppressed people living with HIV (PLWH)	201:330	BACKGROUND Improved point-of-care diagnostic tests for tuberculosis (TB) in severe immune suppressed people living with HIV (PLWH) are needed to decrease morbidity and mortality outcomes.
33076996	3	64	theme	risk	661:664	arg1	factors					666:672	its risk factors	657:672	its risk factors	657:672	We further determined TB incidence, and mortality rates and its risk factors in PLWH with TB symptoms.
33076996	2	65	theme	primary	562:568	arg1	clinics					575:581	primary care clinics	562:581	primary care clinics in Guatemala	562:594	The aim of the study is to evaluate the performance of the lipoarabinomannan antigen test (LAM-test) with and without α-mannosidase pre-treated urine in a cohort of PLWH in primary care clinics in Guatemala.
33076996	2	66	from	PLWH	554:557	arg1	clinics					575:581	primary care clinics	562:581	primary care clinics in Guatemala	562:594	The aim of the study is to evaluate the performance of the lipoarabinomannan antigen test (LAM-test) with and without α-mannosidase pre-treated urine in a cohort of PLWH in primary care clinics in Guatemala.
33076996	10	67	theme	%	1460:1460	arg1	%					1455:1455	of 62.2%	1448:1455	of 62.2% (95% CI 46.5-76.2)	1448:1474	LAM-test sensitivity in PLWH with < 200 CD4 T cells/µl was of 62.2% (95% CI 46.5-76.2).
33076996	10	67	theme	%	1460:1460	arg1	CI					1462:1463	95% CI 46.5-76.2	1458:1473	95% CI 46.5-76.2	1458:1473	LAM-test sensitivity in PLWH with < 200 CD4 T cells/µl was of 62.2% (95% CI 46.5-76.2).
33076996	11	68	theme	untreated	1576:1584	arg1	%					1623:1623	treated urine [55.2%	1604:1623	untreated vs. α-mannosidase treated urine [55.2% (95% CI 42.6-67.4)	1576:1642	There were no significant differences in sensitivity when comparing LAM-test results obtained from untreated vs. α-mannosidase treated urine [55.2% (95% CI 42.6-67.4) vs. 56.9% (95% CI 44-69.2), respectively].
33076996	11	68	theme	untreated	1576:1584	arg1	CI					1630:1631	95% CI 42.6-67.4	1626:1641	95% CI 42.6-67.4	1626:1641	There were no significant differences in sensitivity when comparing LAM-test results obtained from untreated vs. α-mannosidase treated urine [55.2% (95% CI 42.6-67.4) vs. 56.9% (95% CI 44-69.2), respectively].
33076996	11	69	from	differences	1503:1513	arg1	sensitivity					1518:1528	sensitivity	1518:1528	sensitivity	1518:1528	There were no significant differences in sensitivity when comparing LAM-test results obtained from untreated vs. α-mannosidase treated urine [55.2% (95% CI 42.6-67.4) vs. 56.9% (95% CI 44-69.2), respectively].
33076996	8	70	theme	43.3-68.3	1277:1285	arg1	CI					1270:1271	95% CI	1266:1271	95% CI of (43.3-68.3)	1266:1286	RESULTS The overall sensitivity of the LAM-test was of 56.1% with 95% CI of (43.3-68.3).
33076996	15	71	theme	LAM-test	2325:2332	arg1	result					2334:2339	a positive LAM-test result	2314:2339	a positive LAM-test result	2314:2339	Importantly, high rates of TB incidence and mortality were found, and a positive LAM-test result predicted mortality in PLWH with TB clinical symptoms.
33076996	9	72	theme	CD4	1370:1372	arg1	values					1381:1386	CD4 T cell values	1370:1386	CD4 T cell values	1370:1386	There were no differences in the LAM-test sensitivity neither by hospital nor by CD4 T cell values.
33076996	6	73	theme	diagnostic	1095:1104	arg1	accuracy					1106:1113	the LAM-test diagnostic accuracy	1082:1113	the LAM-test diagnostic accuracy studies	1082:1121	A composite reference standard of either a positive Mycobacterium tuberculosis complex culture and/or GeneXpert® MTB/RIF (Xpert, Cepheid, Sunnyvale, CA, USA) results was used in the LAM-test diagnostic accuracy studies.
33076996	14	74	theme	α-mannosidase	2057:2069	arg1	treatment					2071:2079	α-mannosidase treatment	2057:2079	α-mannosidase treatment of urine	2057:2088	CONCLUSIONS In this study, α-mannosidase treatment of urine did not significantly increase the LAM-test performance, however; this needs to be further evaluated in a large-scale study due to our study limitations.
33076996	1	75	theme	BACKGROUND	201:210	arg1	tests					246:250	BACKGROUND Improved point-of-care diagnostic tests	201:250	BACKGROUND Improved point-of-care diagnostic tests for tuberculosis (TB) in severe immune suppressed people living with HIV (PLWH)	201:330	BACKGROUND Improved point-of-care diagnostic tests for tuberculosis (TB) in severe immune suppressed people living with HIV (PLWH) are needed to decrease morbidity and mortality outcomes.
33076996	12	76	theme	mortality	1773:1781	arg1	rate					1783:1786	mortality rate	1773:1786	mortality rate	1773:1786	TB incidence in our cohort was of 21.4/100 person years (PYs) (95% CI 16.6-27.6), and mortality rate was of 11.1/100 PYs (95% CI 8.2-15.0).
33076996	6	77	theme	standard	926:933	arg1	reference					916:924	A composite reference standard	904:933	A composite reference standard of either a positive Mycobacterium tuberculosis complex culture and/or GeneXpert® MTB/RIF (Xpert, Cepheid, Sunnyvale, CA, USA) results	904:1068	A composite reference standard of either a positive Mycobacterium tuberculosis complex culture and/or GeneXpert® MTB/RIF (Xpert, Cepheid, Sunnyvale, CA, USA) results was used in the LAM-test diagnostic accuracy studies.
33076996	4	78	with	study	733:737	arg1	symptoms					755:762	TB symptoms	752:762	TB symptoms	752:762	METHODS Prospective longitudinal study of PLWH with TB symptoms.
33076996	13	79	theme	adjusted	1884:1891	arg1	aHR					1907:1909	aHR	1907:1909	aHR	1907:1909	Importantly, PLWH with a positive LAM-test result had an adjusted hazard ratio (aHR) of death of 1.98 (1.0-3.8) with a significant p value of 0.044 when compared to PLWH with a negative LAM-test result.
33076996	13	79	theme	adjusted	1884:1891	arg1	ratio					1900:1904	an adjusted hazard ratio	1881:1904	an adjusted hazard ratio (aHR) of death of 1.98 (1.0-3.8) with a significant p value of 0.044	1881:1973	Importantly, PLWH with a positive LAM-test result had an adjusted hazard ratio (aHR) of death of 1.98 (1.0-3.8) with a significant p value of 0.044 when compared to PLWH with a negative LAM-test result.
33076996	1	80	theme	point-of-care	221:233	arg1	tests					246:250	BACKGROUND Improved point-of-care diagnostic tests	201:250	BACKGROUND Improved point-of-care diagnostic tests for tuberculosis (TB) in severe immune suppressed people living with HIV (PLWH)	201:330	BACKGROUND Improved point-of-care diagnostic tests for tuberculosis (TB) in severe immune suppressed people living with HIV (PLWH) are needed to decrease morbidity and mortality outcomes.
33076996	12	81	theme	%	1752:1752	arg1	years					1737:1741	21.4/100 person years	1721:1741	21.4/100 person years (PYs) (95% CI 16.6-27.6)	1721:1766	TB incidence in our cohort was of 21.4/100 person years (PYs) (95% CI 16.6-27.6), and mortality rate was of 11.1/100 PYs (95% CI 8.2-15.0).
33076996	12	81	theme	%	1752:1752	arg1	CI					1754:1755	95% CI 16.6-27.6	1750:1765	95% CI 16.6-27.6	1750:1765	TB incidence in our cohort was of 21.4/100 person years (PYs) (95% CI 16.6-27.6), and mortality rate was of 11.1/100 PYs (95% CI 8.2-15.0).
33076996	6	82	theme	composite	906:914	arg1	reference					916:924	A composite reference standard	904:933	A composite reference standard of either a positive Mycobacterium tuberculosis complex culture and/or GeneXpert® MTB/RIF (Xpert, Cepheid, Sunnyvale, CA, USA) results	904:1068	A composite reference standard of either a positive Mycobacterium tuberculosis complex culture and/or GeneXpert® MTB/RIF (Xpert, Cepheid, Sunnyvale, CA, USA) results was used in the LAM-test diagnostic accuracy studies.
33076996	2	83	theme	pre-treated	521:531	arg1	urine					533:537	α-mannosidase pre-treated urine	507:537	α-mannosidase pre-treated urine in a cohort of PLWH in primary care clinics in Guatemala	507:594	The aim of the study is to evaluate the performance of the lipoarabinomannan antigen test (LAM-test) with and without α-mannosidase pre-treated urine in a cohort of PLWH in primary care clinics in Guatemala.
33076996	0	84	theme	people	164:169	arg1	cohort					154:159	a cohort	152:159	a cohort of people living with HIV in Guatemala	152:198	Accuracy of the tuberculosis point-of-care Alere determine lipoarabinomannan antigen diagnostic test using α-mannosidase treated and untreated urine in a cohort of people living with HIV in Guatemala.
33076996	2	85	theme	PLWH	554:557	arg1	cohort					544:549	a cohort	542:549	a cohort of PLWH in primary care clinics in Guatemala	542:594	The aim of the study is to evaluate the performance of the lipoarabinomannan antigen test (LAM-test) with and without α-mannosidase pre-treated urine in a cohort of PLWH in primary care clinics in Guatemala.
33076996	13	86	theme	LAM-test	1861:1868	arg1	result					1870:1875	a positive LAM-test result	1850:1875	a positive LAM-test result	1850:1875	Importantly, PLWH with a positive LAM-test result had an adjusted hazard ratio (aHR) of death of 1.98 (1.0-3.8) with a significant p value of 0.044 when compared to PLWH with a negative LAM-test result.
33076996	4	87	dep	METHODS	700:706	arg1	study					733:737	Prospective longitudinal study	708:737	METHODS Prospective longitudinal study of PLWH with TB symptoms	700:762	METHODS Prospective longitudinal study of PLWH with TB symptoms.
33076996	6	88	theme	GeneXpert®	1006:1015	arg1	MTB/RIF					1017:1023	GeneXpert® MTB/RIF	1006:1023	GeneXpert® MTB/RIF (Xpert, Cepheid, Sunnyvale, CA, USA)	1006:1060	A composite reference standard of either a positive Mycobacterium tuberculosis complex culture and/or GeneXpert® MTB/RIF (Xpert, Cepheid, Sunnyvale, CA, USA) results was used in the LAM-test diagnostic accuracy studies.
33076996	15	89	theme	mortality	2288:2296	arg1	rates					2262:2266	high rates	2257:2266	high rates of TB incidence and mortality	2257:2296	Importantly, high rates of TB incidence and mortality were found, and a positive LAM-test result predicted mortality in PLWH with TB clinical symptoms.
33076996	7	90	theme	mortality	1178:1186	arg1	predictors					1188:1197	mortality predictors	1178:1197	mortality predictors	1178:1197	Cox proportional hazards regression was used to study mortality predictors.
33076996	13	91	with	PLWH	1840:1843	arg1	result					1870:1875	a positive LAM-test result	1850:1875	a positive LAM-test result	1850:1875	Importantly, PLWH with a positive LAM-test result had an adjusted hazard ratio (aHR) of death of 1.98 (1.0-3.8) with a significant p value of 0.044 when compared to PLWH with a negative LAM-test result.
33076996	9	92	theme	cell	1376:1379	arg1	values					1381:1386	CD4 T cell values	1370:1386	CD4 T cell values	1370:1386	There were no differences in the LAM-test sensitivity neither by hospital nor by CD4 T cell values.
33076996	13	93	theme	0.044	1969:1973	arg1	value					1960:1964	a significant p value	1944:1964	a significant p value of 0.044	1944:1973	Importantly, PLWH with a positive LAM-test result had an adjusted hazard ratio (aHR) of death of 1.98 (1.0-3.8) with a significant p value of 0.044 when compared to PLWH with a negative LAM-test result.
33076996	6	94	theme	culture	991:997	arg1	reference					916:924	A composite reference standard	904:933	A composite reference standard of either a positive Mycobacterium tuberculosis complex culture and/or GeneXpert® MTB/RIF (Xpert, Cepheid, Sunnyvale, CA, USA) results	904:1068	A composite reference standard of either a positive Mycobacterium tuberculosis complex culture and/or GeneXpert® MTB/RIF (Xpert, Cepheid, Sunnyvale, CA, USA) results was used in the LAM-test diagnostic accuracy studies.
33076996	15	95	theme	TB	2374:2375	arg1	symptoms					2386:2393	TB clinical symptoms	2374:2393	TB clinical symptoms	2374:2393	Importantly, high rates of TB incidence and mortality were found, and a positive LAM-test result predicted mortality in PLWH with TB clinical symptoms.
33076996	6	96	theme	tuberculosis	970:981	arg1	culture					991:997	a positive Mycobacterium tuberculosis complex culture	945:997	a positive Mycobacterium tuberculosis complex culture	945:997	A composite reference standard of either a positive Mycobacterium tuberculosis complex culture and/or GeneXpert® MTB/RIF (Xpert, Cepheid, Sunnyvale, CA, USA) results was used in the LAM-test diagnostic accuracy studies.
33076996	2	97	theme	test	474:477	arg1	performance					429:439	the performance	425:439	the performance of the lipoarabinomannan antigen test (LAM-test) with and without α-mannosidase pre-treated urine in a cohort of PLWH in primary care clinics in Guatemala	425:594	The aim of the study is to evaluate the performance of the lipoarabinomannan antigen test (LAM-test) with and without α-mannosidase pre-treated urine in a cohort of PLWH in primary care clinics in Guatemala.
33076996	8	98	theme	%	1268:1268	arg1	CI					1270:1271	95% CI	1266:1271	95% CI of (43.3-68.3)	1266:1286	RESULTS The overall sensitivity of the LAM-test was of 56.1% with 95% CI of (43.3-68.3).
33076996	0	99	theme	treated	121:127	arg1	urine					143:147	α-mannosidase treated and untreated urine	107:147	α-mannosidase treated and untreated urine	107:147	Accuracy of the tuberculosis point-of-care Alere determine lipoarabinomannan antigen diagnostic test using α-mannosidase treated and untreated urine in a cohort of people living with HIV in Guatemala.
33076996	5	100	from	urine	852:856	arg1	sensitivity					821:831	the sensitivity	817:831	the sensitivity of the LAM-test in urine with and without α-mannosidase pre-treatment	817:901	Urine samples were collected at 2 HIV sites to test the sensitivity of the LAM-test in urine with and without α-mannosidase pre-treatment.
33076996	13	101	theme	LAM-test	2013:2020	arg1	result					2022:2027	a negative LAM-test result	2002:2027	a negative LAM-test result	2002:2027	Importantly, PLWH with a positive LAM-test result had an adjusted hazard ratio (aHR) of death of 1.98 (1.0-3.8) with a significant p value of 0.044 when compared to PLWH with a negative LAM-test result.
33076996	6	102	theme	positive	947:954	arg1	culture					991:997	a positive Mycobacterium tuberculosis complex culture	945:997	a positive Mycobacterium tuberculosis complex culture	945:997	A composite reference standard of either a positive Mycobacterium tuberculosis complex culture and/or GeneXpert® MTB/RIF (Xpert, Cepheid, Sunnyvale, CA, USA) results was used in the LAM-test diagnostic accuracy studies.
33076996	6	103	dep	culture	991:997	arg1	results					1062:1068	results	1062:1068	results	1062:1068	A composite reference standard of either a positive Mycobacterium tuberculosis complex culture and/or GeneXpert® MTB/RIF (Xpert, Cepheid, Sunnyvale, CA, USA) results was used in the LAM-test diagnostic accuracy studies.
33076996	6	104	dep	MTB/RIF	1017:1023	arg1	USA					1057:1059	USA	1057:1059	USA	1057:1059	A composite reference standard of either a positive Mycobacterium tuberculosis complex culture and/or GeneXpert® MTB/RIF (Xpert, Cepheid, Sunnyvale, CA, USA) results was used in the LAM-test diagnostic accuracy studies.
33076996	6	104	dep	MTB/RIF	1017:1023	arg1	CA					1053:1054	CA	1053:1054	CA	1053:1054	A composite reference standard of either a positive Mycobacterium tuberculosis complex culture and/or GeneXpert® MTB/RIF (Xpert, Cepheid, Sunnyvale, CA, USA) results was used in the LAM-test diagnostic accuracy studies.
33076996	6	104	dep	MTB/RIF	1017:1023	arg1	Sunnyvale					1042:1050	Sunnyvale	1042:1050	Sunnyvale	1042:1050	A composite reference standard of either a positive Mycobacterium tuberculosis complex culture and/or GeneXpert® MTB/RIF (Xpert, Cepheid, Sunnyvale, CA, USA) results was used in the LAM-test diagnostic accuracy studies.
33076996	6	104	dep	MTB/RIF	1017:1023	arg1	Cepheid					1033:1039	Cepheid	1033:1039	Cepheid	1033:1039	A composite reference standard of either a positive Mycobacterium tuberculosis complex culture and/or GeneXpert® MTB/RIF (Xpert, Cepheid, Sunnyvale, CA, USA) results was used in the LAM-test diagnostic accuracy studies.
33076996	0	105	theme	untreated	133:141	arg1	urine					143:147	α-mannosidase treated and untreated urine	107:147	α-mannosidase treated and untreated urine	107:147	Accuracy of the tuberculosis point-of-care Alere determine lipoarabinomannan antigen diagnostic test using α-mannosidase treated and untreated urine in a cohort of people living with HIV in Guatemala.
33076996	1	106	theme	morbidity	355:363	arg1	outcomes					379:386	morbidity and mortality outcomes	355:386	morbidity and mortality outcomes	355:386	BACKGROUND Improved point-of-care diagnostic tests for tuberculosis (TB) in severe immune suppressed people living with HIV (PLWH) are needed to decrease morbidity and mortality outcomes.
33076996	12	107	theme	person	1730:1735	arg1	years					1737:1741	21.4/100 person years	1721:1741	21.4/100 person years (PYs) (95% CI 16.6-27.6)	1721:1766	TB incidence in our cohort was of 21.4/100 person years (PYs) (95% CI 16.6-27.6), and mortality rate was of 11.1/100 PYs (95% CI 8.2-15.0).
33076996	12	107	theme	person	1730:1735	arg1	CI					1754:1755	95% CI 16.6-27.6	1750:1765	95% CI 16.6-27.6	1750:1765	TB incidence in our cohort was of 21.4/100 person years (PYs) (95% CI 16.6-27.6), and mortality rate was of 11.1/100 PYs (95% CI 8.2-15.0).
33076996	12	107	theme	person	1730:1735	arg1	PYs					1744:1746	PYs	1744:1746	PYs	1744:1746	TB incidence in our cohort was of 21.4/100 person years (PYs) (95% CI 16.6-27.6), and mortality rate was of 11.1/100 PYs (95% CI 8.2-15.0).
33076996	5	108	from	LAM-test	840:847	arg1	urine					852:856	urine	852:856	urine	852:856	Urine samples were collected at 2 HIV sites to test the sensitivity of the LAM-test in urine with and without α-mannosidase pre-treatment.
33076996	4	109	theme	PLWH	742:745	arg1	study					733:737	Prospective longitudinal study	708:737	METHODS Prospective longitudinal study of PLWH with TB symptoms	700:762	METHODS Prospective longitudinal study of PLWH with TB symptoms.
33076996	7	110	theme	hazards	1141:1147	arg1	regression					1149:1158	Cox proportional hazards regression	1124:1158	Cox proportional hazards regression	1124:1158	Cox proportional hazards regression was used to study mortality predictors.
33076996	7	111	theme	Cox	1124:1126	arg1	regression					1149:1158	Cox proportional hazards regression	1124:1158	Cox proportional hazards regression	1124:1158	Cox proportional hazards regression was used to study mortality predictors.
33076996	1	112	theme	mortality	369:377	arg1	outcomes					379:386	morbidity and mortality outcomes	355:386	morbidity and mortality outcomes	355:386	BACKGROUND Improved point-of-care diagnostic tests for tuberculosis (TB) in severe immune suppressed people living with HIV (PLWH) are needed to decrease morbidity and mortality outcomes.
33076996	7	113	used	used	1164:1167	arg2	regression					1149:1158	Cox proportional hazards regression	1124:1158	Cox proportional hazards regression	1124:1158	Cox proportional hazards regression was used to study mortality predictors.
33076996	0	114	theme	tuberculosis	16:27	arg1	Alere					43:47	the tuberculosis point-of-care Alere	12:47	the tuberculosis point-of-care Alere	12:47	Accuracy of the tuberculosis point-of-care Alere determine lipoarabinomannan antigen diagnostic test using α-mannosidase treated and untreated urine in a cohort of people living with HIV in Guatemala.
33076996	1	115	theme	suppressed	291:300	arg1	people					302:307	severe immune suppressed people	277:307	severe immune suppressed people living with HIV (PLWH)	277:330	BACKGROUND Improved point-of-care diagnostic tests for tuberculosis (TB) in severe immune suppressed people living with HIV (PLWH) are needed to decrease morbidity and mortality outcomes.
33076996	8	116	dep	RESULTS	1200:1206	arg1	%					1259:1259	of 56.1%	1252:1259	of 56.1%	1252:1259	RESULTS The overall sensitivity of the LAM-test was of 56.1% with 95% CI of (43.3-68.3).
33076996	8	116	dep	RESULTS	1200:1206	arg1	sensitivity					1220:1230	The overall sensitivity	1208:1230	The overall sensitivity of the LAM-test	1208:1246	RESULTS The overall sensitivity of the LAM-test was of 56.1% with 95% CI of (43.3-68.3).
33076996	10	117	theme	PLWH	1413:1416	arg1	cells/µl					1435:1442	PLWH with < 200 CD4 T cells/µl	1413:1442	PLWH with < 200 CD4 T cells/µl	1413:1442	LAM-test sensitivity in PLWH with < 200 CD4 T cells/µl was of 62.2% (95% CI 46.5-76.2).
33076996	8	118	with	%	1259:1259	arg1	CI					1270:1271	95% CI	1266:1271	95% CI of (43.3-68.3)	1266:1286	RESULTS The overall sensitivity of the LAM-test was of 56.1% with 95% CI of (43.3-68.3).
33076996	4	119	theme	Prospective	708:718	arg1	study					733:737	Prospective longitudinal study	708:737	METHODS Prospective longitudinal study of PLWH with TB symptoms	700:762	METHODS Prospective longitudinal study of PLWH with TB symptoms.
33076996	2	120	theme	study	404:408	arg1	aim					393:395	The aim	389:395	The aim of the study	389:408	The aim of the study is to evaluate the performance of the lipoarabinomannan antigen test (LAM-test) with and without α-mannosidase pre-treated urine in a cohort of PLWH in primary care clinics in Guatemala.
33076996	0	121	theme	Alere	43:47	arg1	Accuracy					0:7	Accuracy	0:7	Accuracy of the tuberculosis point-of-care Alere	0:47	Accuracy of the tuberculosis point-of-care Alere determine lipoarabinomannan antigen diagnostic test using α-mannosidase treated and untreated urine in a cohort of people living with HIV in Guatemala.
33076996	2	122	theme	lipoarabinomannan	448:464	arg1	LAM-test					480:487	LAM-test	480:487	LAM-test	480:487	The aim of the study is to evaluate the performance of the lipoarabinomannan antigen test (LAM-test) with and without α-mannosidase pre-treated urine in a cohort of PLWH in primary care clinics in Guatemala.
33076996	2	122	theme	lipoarabinomannan	448:464	arg1	test					474:477	the lipoarabinomannan antigen test	444:477	the lipoarabinomannan antigen test (LAM-test)	444:488	The aim of the study is to evaluate the performance of the lipoarabinomannan antigen test (LAM-test) with and without α-mannosidase pre-treated urine in a cohort of PLWH in primary care clinics in Guatemala.
33076996	0	123	theme	lipoarabinomannan	59:75	arg1	test					96:99	lipoarabinomannan antigen diagnostic test	59:99	lipoarabinomannan antigen diagnostic test using α-mannosidase treated and untreated urine in a cohort of people living with HIV in Guatemala	59:198	Accuracy of the tuberculosis point-of-care Alere determine lipoarabinomannan antigen diagnostic test using α-mannosidase treated and untreated urine in a cohort of people living with HIV in Guatemala.
33076996	12	124	theme	TB	1687:1688	arg1	incidence					1690:1698	TB incidence	1687:1698	TB incidence in our cohort	1687:1712	TB incidence in our cohort was of 21.4/100 person years (PYs) (95% CI 16.6-27.6), and mortality rate was of 11.1/100 PYs (95% CI 8.2-15.0).
33076996	3	125	with	PLWH	677:680	arg1	symptoms					690:697	TB symptoms	687:697	TB symptoms	687:697	We further determined TB incidence, and mortality rates and its risk factors in PLWH with TB symptoms.
33076996	2	126	from	urine	533:537	arg1	cohort					544:549	a cohort	542:549	a cohort of PLWH in primary care clinics in Guatemala	542:594	The aim of the study is to evaluate the performance of the lipoarabinomannan antigen test (LAM-test) with and without α-mannosidase pre-treated urine in a cohort of PLWH in primary care clinics in Guatemala.
33076996	10	127	theme	CD4	1429:1431	arg1	cells/µl					1435:1442	PLWH with < 200 CD4 T cells/µl	1413:1442	PLWH with < 200 CD4 T cells/µl	1413:1442	LAM-test sensitivity in PLWH with < 200 CD4 T cells/µl was of 62.2% (95% CI 46.5-76.2).
33076996	4	128	theme	TB	752:753	arg1	symptoms					755:762	TB symptoms	752:762	TB symptoms	752:762	METHODS Prospective longitudinal study of PLWH with TB symptoms.
33076996	15	129	theme	incidence	2274:2282	arg1	rates					2262:2266	high rates	2257:2266	high rates of TB incidence and mortality	2257:2296	Importantly, high rates of TB incidence and mortality were found, and a positive LAM-test result predicted mortality in PLWH with TB clinical symptoms.
33076996	14	130	theme	due	2214:2216	arg1	study					2208:2212	a large-scale study	2194:2212	a large-scale study due to our study limitations	2194:2241	CONCLUSIONS In this study, α-mannosidase treatment of urine did not significantly increase the LAM-test performance, however; this needs to be further evaluated in a large-scale study due to our study limitations.
33076996	14	131	theme	LAM-test	2125:2132	arg1	performance					2134:2144	the LAM-test performance	2121:2144	the LAM-test performance	2121:2144	CONCLUSIONS In this study, α-mannosidase treatment of urine did not significantly increase the LAM-test performance, however; this needs to be further evaluated in a large-scale study due to our study limitations.
33076996	9	132	theme	LAM-test	1322:1329	arg1	sensitivity					1331:1341	the LAM-test sensitivity	1318:1341	the LAM-test sensitivity	1318:1341	There were no differences in the LAM-test sensitivity neither by hospital nor by CD4 T cell values.
32777429	0	0	theme	safe	95:98	arg1	effect					119:124	a safe ocular hypotensive effect	93:124	a safe ocular hypotensive effect	93:124	Polypeptide and glycosaminoglycan polysaccharide as stabilizing polymers in nanocrystals for a safe ocular hypotensive effect.
32777429	8	1	theme	modified	1170:1177	arg1	test					1186:1189	The modified Draize test	1166:1189	The modified Draize test	1166:1189	The modified Draize test proved the safety and tolerability following application to rabbit eyes accompanying an efficient ocular hypotensive activity using a steroid glaucoma model.
32777429	7	2	theme	powder	1066:1071	arg1	XRPD					1086:1089	XRPD	1086:1089	XRPD	1086:1089	Particles crystallinity was confirmed using X-ray powder diffraction (XRPD) and differential scanning calorimetry (DSC) in liquid and spray dried NS.
32777429	7	2	theme	powder	1066:1071	arg1	diffraction					1073:1083	X-ray powder diffraction	1060:1083	X-ray powder diffraction (XRPD)	1060:1090	Particles crystallinity was confirmed using X-ray powder diffraction (XRPD) and differential scanning calorimetry (DSC) in liquid and spray dried NS.
32777429	1	3	theme	soluble	164:170	arg1	acetazolamide					192:204	acetazolamide	192:204	acetazolamide (ACZ)	192:210	Improved ocular delivery of a poorly soluble anti-glaucoma drug, acetazolamide (ACZ), in a stable nanosuspension (NS) was the main target of the study.
32777429	1	3	theme	soluble	164:170	arg1	drug					186:189	a poorly soluble anti-glaucoma drug	155:189	a poorly soluble anti-glaucoma drug	155:189	Improved ocular delivery of a poorly soluble anti-glaucoma drug, acetazolamide (ACZ), in a stable nanosuspension (NS) was the main target of the study.
32777429	2	4	theme	hyaluronic	357:366	arg1	glycosaminoglycan					338:354	the glycosaminoglycan	334:354	the glycosaminoglycan	334:354	The anionic polypeptide, poly-γ-glutamic acid (PG) and the glycosaminoglycan, hyaluronic acid, were used to stabilize ACZ-NS prepared using the antisolvent precipitation (AS-PT) coupled with sonication technique.
32777429	2	4	theme	hyaluronic	357:366	arg1	acid					368:371	hyaluronic acid	357:371	hyaluronic acid	357:371	The anionic polypeptide, poly-γ-glutamic acid (PG) and the glycosaminoglycan, hyaluronic acid, were used to stabilize ACZ-NS prepared using the antisolvent precipitation (AS-PT) coupled with sonication technique.
32777429	5	5	theme	NS	842:843	arg1	processing					828:837	post-production processing	812:837	post-production processing of NS	812:843	Targeting solvent removal with control on future particle growth, post-production processing of NS was done using spray drying.
32777429	1	6	theme	anti-glaucoma	172:184	arg1	acetazolamide					192:204	acetazolamide	192:204	acetazolamide (ACZ)	192:210	Improved ocular delivery of a poorly soluble anti-glaucoma drug, acetazolamide (ACZ), in a stable nanosuspension (NS) was the main target of the study.
32777429	1	6	theme	anti-glaucoma	172:184	arg1	drug					186:189	a poorly soluble anti-glaucoma drug	155:189	a poorly soluble anti-glaucoma drug	155:189	Improved ocular delivery of a poorly soluble anti-glaucoma drug, acetazolamide (ACZ), in a stable nanosuspension (NS) was the main target of the study.
32777429	0	7	theme	hypotensive	107:117	arg1	effect					119:124	a safe ocular hypotensive effect	93:124	a safe ocular hypotensive effect	93:124	Polypeptide and glycosaminoglycan polysaccharide as stabilizing polymers in nanocrystals for a safe ocular hypotensive effect.
32777429	8	8	theme	efficient	1279:1287	arg1	activity					1308:1315	an efficient ocular hypotensive activity	1276:1315	an efficient ocular hypotensive activity using a steroid glaucoma model	1276:1346	The modified Draize test proved the safety and tolerability following application to rabbit eyes accompanying an efficient ocular hypotensive activity using a steroid glaucoma model.
32777429	0	9	theme	ocular	100:105	arg1	effect					119:124	a safe ocular hypotensive effect	93:124	a safe ocular hypotensive effect	93:124	Polypeptide and glycosaminoglycan polysaccharide as stabilizing polymers in nanocrystals for a safe ocular hypotensive effect.
32777429	7	10	theme	X-ray	1060:1064	arg1	XRPD					1086:1089	XRPD	1086:1089	XRPD	1086:1089	Particles crystallinity was confirmed using X-ray powder diffraction (XRPD) and differential scanning calorimetry (DSC) in liquid and spray dried NS.
32777429	7	10	theme	X-ray	1060:1064	arg1	diffraction					1073:1083	X-ray powder diffraction	1060:1083	X-ray powder diffraction (XRPD)	1060:1090	Particles crystallinity was confirmed using X-ray powder diffraction (XRPD) and differential scanning calorimetry (DSC) in liquid and spray dried NS.
32777429	3	11	theme	site	504:507	arg1	biocompatibility					509:524	site biocompatibility	504:524	site biocompatibility	504:524	To endue in site biocompatibility with high tolerability, soya lecithin (SL) phospholipid has been also combined with polyvinyl alcohol (PVA).
32777429	5	12	theme	particle	795:802	arg1	growth					804:809	future particle growth	788:809	future particle growth	788:809	Targeting solvent removal with control on future particle growth, post-production processing of NS was done using spray drying.
32777429	2	13	theme	sonication	470:479	arg1	technique					481:489	sonication technique	470:489	sonication technique	470:489	The anionic polypeptide, poly-γ-glutamic acid (PG) and the glycosaminoglycan, hyaluronic acid, were used to stabilize ACZ-NS prepared using the antisolvent precipitation (AS-PT) coupled with sonication technique.
32777429	4	14	with	NS	635:636	arg1	PS					651:652	uniform PS	643:652	uniform PS in the range 100-300 nm	643:676	NS with uniform PS in the range 100-300 nm, high ζ > ±20 mV, and enhanced saturation solubility were produced.
32777429	4	14	with	NS	635:636	arg1	solubility					720:729	enhanced saturation solubility	700:729	enhanced saturation solubility	700:729	NS with uniform PS in the range 100-300 nm, high ζ > ±20 mV, and enhanced saturation solubility were produced.
32777429	4	14	with	NS	635:636	arg1	ζ > ±20 mV					684:693	high ζ > ±20 mV	679:693	high ζ > ±20 mV	679:693	NS with uniform PS in the range 100-300 nm, high ζ > ±20 mV, and enhanced saturation solubility were produced.
32777429	4	15	from	ζ > ±20 mV	684:693	arg1	100-300 nm					667:676	the range 100-300 nm	657:676	the range 100-300 nm	657:676	NS with uniform PS in the range 100-300 nm, high ζ > ±20 mV, and enhanced saturation solubility were produced.
32777429	4	16	theme	enhanced	700:707	arg1	solubility					720:729	enhanced saturation solubility	700:729	enhanced saturation solubility	700:729	NS with uniform PS in the range 100-300 nm, high ζ > ±20 mV, and enhanced saturation solubility were produced.
32777429	8	17	theme	Draize	1179:1184	arg1	test					1186:1189	The modified Draize test	1166:1189	The modified Draize test	1166:1189	The modified Draize test proved the safety and tolerability following application to rabbit eyes accompanying an efficient ocular hypotensive activity using a steroid glaucoma model.
32777429	1	18	theme	drug	186:189	arg1	delivery					143:150	Improved ocular delivery	127:150	Improved ocular delivery of a poorly soluble anti-glaucoma drug, acetazolamide (ACZ), in a stable nanosuspension (NS)	127:243	Improved ocular delivery of a poorly soluble anti-glaucoma drug, acetazolamide (ACZ), in a stable nanosuspension (NS) was the main target of the study.
32777429	1	18	theme	drug	186:189	arg1	target					258:263	the main target	249:263	the main target of the study	249:276	Improved ocular delivery of a poorly soluble anti-glaucoma drug, acetazolamide (ACZ), in a stable nanosuspension (NS) was the main target of the study.
32777429	1	19	from	delivery	143:150	arg1	nanosuspension					225:238	a stable nanosuspension	216:238	a stable nanosuspension (NS)	216:243	Improved ocular delivery of a poorly soluble anti-glaucoma drug, acetazolamide (ACZ), in a stable nanosuspension (NS) was the main target of the study.
32777429	1	19	from	delivery	143:150	arg1	NS					241:242	NS	241:242	NS	241:242	Improved ocular delivery of a poorly soluble anti-glaucoma drug, acetazolamide (ACZ), in a stable nanosuspension (NS) was the main target of the study.
32777429	8	20	theme	ocular	1289:1294	arg1	activity					1308:1315	an efficient ocular hypotensive activity	1276:1315	an efficient ocular hypotensive activity using a steroid glaucoma model	1276:1346	The modified Draize test proved the safety and tolerability following application to rabbit eyes accompanying an efficient ocular hypotensive activity using a steroid glaucoma model.
32777429	6	21	theme	powder	1008:1013	arg1	production					962:971	the production	958:971	the production of a redispersible dry crystalline powder	958:1013	The carriers' composition and amount relative to ACZ-NS were optimized to allow for the production of a redispersible dry crystalline powder.
32777429	7	22	theme	spray	1150:1154	arg1	NS					1162:1163	spray dried NS	1150:1163	spray dried NS	1150:1163	Particles crystallinity was confirmed using X-ray powder diffraction (XRPD) and differential scanning calorimetry (DSC) in liquid and spray dried NS.
32777429	7	23	theme	Particles	1016:1024	arg1	crystallinity					1026:1038	Particles crystallinity	1016:1038	Particles crystallinity	1016:1038	Particles crystallinity was confirmed using X-ray powder diffraction (XRPD) and differential scanning calorimetry (DSC) in liquid and spray dried NS.
32777429	6	24	theme	relative	911:918	arg1	amount					904:909	amount	904:909	amount relative to ACZ-NS	904:928	The carriers' composition and amount relative to ACZ-NS were optimized to allow for the production of a redispersible dry crystalline powder.
32777429	2	25	theme	anionic	283:289	arg1	polypeptide					291:301	The anionic polypeptide	279:301	The anionic polypeptide	279:301	The anionic polypeptide, poly-γ-glutamic acid (PG) and the glycosaminoglycan, hyaluronic acid, were used to stabilize ACZ-NS prepared using the antisolvent precipitation (AS-PT) coupled with sonication technique.
32777429	6	26	theme	crystalline	996:1006	arg1	powder					1008:1013	a redispersible dry crystalline powder	976:1013	a redispersible dry crystalline powder	976:1013	The carriers' composition and amount relative to ACZ-NS were optimized to allow for the production of a redispersible dry crystalline powder.
32777429	0	27	theme	Polypeptide	0:10	arg1	polysaccharide					34:47	Polypeptide and glycosaminoglycan polysaccharide	0:47	polysaccharide	34:47	Polypeptide and glycosaminoglycan polysaccharide as stabilizing polymers in nanocrystals for a safe ocular hypotensive effect.
32777429	1	28	theme	main	253:256	arg1	delivery					143:150	Improved ocular delivery	127:150	Improved ocular delivery of a poorly soluble anti-glaucoma drug, acetazolamide (ACZ), in a stable nanosuspension (NS)	127:243	Improved ocular delivery of a poorly soluble anti-glaucoma drug, acetazolamide (ACZ), in a stable nanosuspension (NS) was the main target of the study.
32777429	1	28	theme	main	253:256	arg1	target					258:263	the main target	249:263	the main target of the study	249:276	Improved ocular delivery of a poorly soluble anti-glaucoma drug, acetazolamide (ACZ), in a stable nanosuspension (NS) was the main target of the study.
32777429	4	29	theme	range	661:665	arg1	100-300 nm					667:676	the range 100-300 nm	657:676	the range 100-300 nm	657:676	NS with uniform PS in the range 100-300 nm, high ζ > ±20 mV, and enhanced saturation solubility were produced.
32777429	5	30	theme	post-production	812:826	arg1	processing					828:837	post-production processing	812:837	post-production processing of NS	812:843	Targeting solvent removal with control on future particle growth, post-production processing of NS was done using spray drying.
32777429	5	31	theme	solvent	756:762	arg1	removal					764:770	solvent removal	756:770	solvent removal	756:770	Targeting solvent removal with control on future particle growth, post-production processing of NS was done using spray drying.
32777429	4	32	theme	high	679:682	arg1	ζ > ±20 mV					684:693	high ζ > ±20 mV	679:693	high ζ > ±20 mV	679:693	NS with uniform PS in the range 100-300 nm, high ζ > ±20 mV, and enhanced saturation solubility were produced.
32777429	6	33	theme	redispersible	978:990	arg1	powder					1008:1013	a redispersible dry crystalline powder	976:1013	a redispersible dry crystalline powder	976:1013	The carriers' composition and amount relative to ACZ-NS were optimized to allow for the production of a redispersible dry crystalline powder.
32777429	0	34	theme	glycosaminoglycan	16:32	arg1	polysaccharide					34:47	Polypeptide and glycosaminoglycan polysaccharide	0:47	polysaccharide	34:47	Polypeptide and glycosaminoglycan polysaccharide as stabilizing polymers in nanocrystals for a safe ocular hypotensive effect.
32777429	8	35	theme	hypotensive	1296:1306	arg1	activity					1308:1315	an efficient ocular hypotensive activity	1276:1315	an efficient ocular hypotensive activity using a steroid glaucoma model	1276:1346	The modified Draize test proved the safety and tolerability following application to rabbit eyes accompanying an efficient ocular hypotensive activity using a steroid glaucoma model.
32777429	8	36	theme	glaucoma	1333:1340	arg1	model					1342:1346	a steroid glaucoma model	1323:1346	a steroid glaucoma model	1323:1346	The modified Draize test proved the safety and tolerability following application to rabbit eyes accompanying an efficient ocular hypotensive activity using a steroid glaucoma model.
32777429	0	37	theme	stabilizing	52:62	arg1	polymers					64:71	stabilizing polymers	52:71	stabilizing polymers	52:71	Polypeptide and glycosaminoglycan polysaccharide as stabilizing polymers in nanocrystals for a safe ocular hypotensive effect.
32777429	2	38	theme	poly-γ-glutamic	304:318	arg1	PG					326:327	PG	326:327	PG	326:327	The anionic polypeptide, poly-γ-glutamic acid (PG) and the glycosaminoglycan, hyaluronic acid, were used to stabilize ACZ-NS prepared using the antisolvent precipitation (AS-PT) coupled with sonication technique.
32777429	2	38	theme	poly-γ-glutamic	304:318	arg1	acid					320:323	poly-γ-glutamic acid	304:323	poly-γ-glutamic acid (PG)	304:328	The anionic polypeptide, poly-γ-glutamic acid (PG) and the glycosaminoglycan, hyaluronic acid, were used to stabilize ACZ-NS prepared using the antisolvent precipitation (AS-PT) coupled with sonication technique.
32777429	3	39	theme	lecithin	555:562	arg1	phospholipid					569:580	soya lecithin (SL) phospholipid	550:580	soya lecithin (SL) phospholipid	550:580	To endue in site biocompatibility with high tolerability, soya lecithin (SL) phospholipid has been also combined with polyvinyl alcohol (PVA).
32777429	6	40	theme	dry	992:994	arg1	powder					1008:1013	a redispersible dry crystalline powder	976:1013	a redispersible dry crystalline powder	976:1013	The carriers' composition and amount relative to ACZ-NS were optimized to allow for the production of a redispersible dry crystalline powder.
32777429	2	41	theme	antisolvent	423:433	arg1	AS-PT					450:454	AS-PT	450:454	AS-PT	450:454	The anionic polypeptide, poly-γ-glutamic acid (PG) and the glycosaminoglycan, hyaluronic acid, were used to stabilize ACZ-NS prepared using the antisolvent precipitation (AS-PT) coupled with sonication technique.
32777429	2	41	theme	antisolvent	423:433	arg1	precipitation					435:447	the antisolvent precipitation	419:447	the antisolvent precipitation (AS-PT) coupled with sonication technique	419:489	The anionic polypeptide, poly-γ-glutamic acid (PG) and the glycosaminoglycan, hyaluronic acid, were used to stabilize ACZ-NS prepared using the antisolvent precipitation (AS-PT) coupled with sonication technique.
32777429	1	42	theme	study	272:276	arg1	delivery					143:150	Improved ocular delivery	127:150	Improved ocular delivery of a poorly soluble anti-glaucoma drug, acetazolamide (ACZ), in a stable nanosuspension (NS)	127:243	Improved ocular delivery of a poorly soluble anti-glaucoma drug, acetazolamide (ACZ), in a stable nanosuspension (NS) was the main target of the study.
32777429	1	42	theme	study	272:276	arg1	target					258:263	the main target	249:263	the main target of the study	249:276	Improved ocular delivery of a poorly soluble anti-glaucoma drug, acetazolamide (ACZ), in a stable nanosuspension (NS) was the main target of the study.
32777429	7	43	theme	differential	1096:1107	arg1	DSC					1131:1133	DSC	1131:1133	DSC	1131:1133	Particles crystallinity was confirmed using X-ray powder diffraction (XRPD) and differential scanning calorimetry (DSC) in liquid and spray dried NS.
32777429	7	43	theme	differential	1096:1107	arg1	calorimetry					1118:1128	differential scanning calorimetry	1096:1128	differential scanning calorimetry (DSC)	1096:1134	Particles crystallinity was confirmed using X-ray powder diffraction (XRPD) and differential scanning calorimetry (DSC) in liquid and spray dried NS.
32777429	5	44	theme	future	788:793	arg1	growth					804:809	future particle growth	788:809	future particle growth	788:809	Targeting solvent removal with control on future particle growth, post-production processing of NS was done using spray drying.
32777429	1	45	theme	Improved	127:134	arg1	delivery					143:150	Improved ocular delivery	127:150	Improved ocular delivery of a poorly soluble anti-glaucoma drug, acetazolamide (ACZ), in a stable nanosuspension (NS)	127:243	Improved ocular delivery of a poorly soluble anti-glaucoma drug, acetazolamide (ACZ), in a stable nanosuspension (NS) was the main target of the study.
32777429	1	45	theme	Improved	127:134	arg1	target					258:263	the main target	249:263	the main target of the study	249:276	Improved ocular delivery of a poorly soluble anti-glaucoma drug, acetazolamide (ACZ), in a stable nanosuspension (NS) was the main target of the study.
32777429	3	46	theme	soya	550:553	arg1	phospholipid					569:580	soya lecithin (SL) phospholipid	550:580	soya lecithin (SL) phospholipid	550:580	To endue in site biocompatibility with high tolerability, soya lecithin (SL) phospholipid has been also combined with polyvinyl alcohol (PVA).
32777429	1	47	theme	ocular	136:141	arg1	delivery					143:150	Improved ocular delivery	127:150	Improved ocular delivery of a poorly soluble anti-glaucoma drug, acetazolamide (ACZ), in a stable nanosuspension (NS)	127:243	Improved ocular delivery of a poorly soluble anti-glaucoma drug, acetazolamide (ACZ), in a stable nanosuspension (NS) was the main target of the study.
32777429	1	47	theme	ocular	136:141	arg1	target					258:263	the main target	249:263	the main target of the study	249:276	Improved ocular delivery of a poorly soluble anti-glaucoma drug, acetazolamide (ACZ), in a stable nanosuspension (NS) was the main target of the study.
32777429	3	48	theme	high	531:534	arg1	tolerability					536:547	high tolerability	531:547	high tolerability	531:547	To endue in site biocompatibility with high tolerability, soya lecithin (SL) phospholipid has been also combined with polyvinyl alcohol (PVA).
32777429	2	49	used	used	379:382	arg2	polypeptide					291:301	The anionic polypeptide	279:301	The anionic polypeptide	279:301	The anionic polypeptide, poly-γ-glutamic acid (PG) and the glycosaminoglycan, hyaluronic acid, were used to stabilize ACZ-NS prepared using the antisolvent precipitation (AS-PT) coupled with sonication technique.
32777429	2	49	used	used	379:382	arg2	PG					326:327	PG	326:327	PG	326:327	The anionic polypeptide, poly-γ-glutamic acid (PG) and the glycosaminoglycan, hyaluronic acid, were used to stabilize ACZ-NS prepared using the antisolvent precipitation (AS-PT) coupled with sonication technique.
32777429	2	49	used	used	379:382	arg2	acid					320:323	poly-γ-glutamic acid	304:323	poly-γ-glutamic acid (PG)	304:328	The anionic polypeptide, poly-γ-glutamic acid (PG) and the glycosaminoglycan, hyaluronic acid, were used to stabilize ACZ-NS prepared using the antisolvent precipitation (AS-PT) coupled with sonication technique.
32777429	2	49	used	used	379:382	arg2	acid					368:371	hyaluronic acid	357:371	hyaluronic acid	357:371	The anionic polypeptide, poly-γ-glutamic acid (PG) and the glycosaminoglycan, hyaluronic acid, were used to stabilize ACZ-NS prepared using the antisolvent precipitation (AS-PT) coupled with sonication technique.
32777429	2	49	used	used	379:382	arg2	glycosaminoglycan					338:354	the glycosaminoglycan	334:354	the glycosaminoglycan	334:354	The anionic polypeptide, poly-γ-glutamic acid (PG) and the glycosaminoglycan, hyaluronic acid, were used to stabilize ACZ-NS prepared using the antisolvent precipitation (AS-PT) coupled with sonication technique.
32777429	5	50	theme	spray	860:864	arg1	drying					866:871	spray drying	860:871	spray drying	860:871	Targeting solvent removal with control on future particle growth, post-production processing of NS was done using spray drying.
32777429	8	51	theme	steroid	1325:1331	arg1	model					1342:1346	a steroid glaucoma model	1323:1346	a steroid glaucoma model	1323:1346	The modified Draize test proved the safety and tolerability following application to rabbit eyes accompanying an efficient ocular hypotensive activity using a steroid glaucoma model.
32777429	5	52	from	control	777:783	arg1	growth					804:809	future particle growth	788:809	future particle growth	788:809	Targeting solvent removal with control on future particle growth, post-production processing of NS was done using spray drying.
32777429	3	53	theme	polyvinyl	610:618	arg1	alcohol					620:626	polyvinyl alcohol	610:626	polyvinyl alcohol (PVA)	610:632	To endue in site biocompatibility with high tolerability, soya lecithin (SL) phospholipid has been also combined with polyvinyl alcohol (PVA).
32777429	3	53	theme	polyvinyl	610:618	arg1	PVA					629:631	PVA	629:631	PVA	629:631	To endue in site biocompatibility with high tolerability, soya lecithin (SL) phospholipid has been also combined with polyvinyl alcohol (PVA).
32777429	4	54	theme	saturation	709:718	arg1	solubility					720:729	enhanced saturation solubility	700:729	enhanced saturation solubility	700:729	NS with uniform PS in the range 100-300 nm, high ζ > ±20 mV, and enhanced saturation solubility were produced.
32777429	8	55	dep	safety	1202:1207	arg1	the					1198:1200	the	1198:1200	the	1198:1200	The modified Draize test proved the safety and tolerability following application to rabbit eyes accompanying an efficient ocular hypotensive activity using a steroid glaucoma model.
32777429	4	56	from	PS	651:652	arg1	100-300 nm					667:676	the range 100-300 nm	657:676	the range 100-300 nm	657:676	NS with uniform PS in the range 100-300 nm, high ζ > ±20 mV, and enhanced saturation solubility were produced.
32777429	1	57	theme	stable	218:223	arg1	nanosuspension					225:238	a stable nanosuspension	216:238	a stable nanosuspension (NS)	216:243	Improved ocular delivery of a poorly soluble anti-glaucoma drug, acetazolamide (ACZ), in a stable nanosuspension (NS) was the main target of the study.
32777429	1	57	theme	stable	218:223	arg1	NS					241:242	NS	241:242	NS	241:242	Improved ocular delivery of a poorly soluble anti-glaucoma drug, acetazolamide (ACZ), in a stable nanosuspension (NS) was the main target of the study.
32777429	7	58	theme	dried	1156:1160	arg1	NS					1162:1163	spray dried NS	1150:1163	spray dried NS	1150:1163	Particles crystallinity was confirmed using X-ray powder diffraction (XRPD) and differential scanning calorimetry (DSC) in liquid and spray dried NS.
32777429	3	59	theme	SL	565:566	arg1	phospholipid					569:580	soya lecithin (SL) phospholipid	550:580	soya lecithin (SL) phospholipid	550:580	To endue in site biocompatibility with high tolerability, soya lecithin (SL) phospholipid has been also combined with polyvinyl alcohol (PVA).
32777429	8	60	theme	rabbit	1251:1256	arg1	eyes					1258:1261	rabbit eyes	1251:1261	rabbit eyes accompanying an efficient ocular hypotensive activity using a steroid glaucoma model	1251:1346	The modified Draize test proved the safety and tolerability following application to rabbit eyes accompanying an efficient ocular hypotensive activity using a steroid glaucoma model.
32777429	0	61	from	polysaccharide	34:47	arg1	nanocrystals					76:87	nanocrystals	76:87	nanocrystals for a safe ocular hypotensive effect	76:124	Polypeptide and glycosaminoglycan polysaccharide as stabilizing polymers in nanocrystals for a safe ocular hypotensive effect.
32777429	7	62	theme	scanning	1109:1116	arg1	DSC					1131:1133	DSC	1131:1133	DSC	1131:1133	Particles crystallinity was confirmed using X-ray powder diffraction (XRPD) and differential scanning calorimetry (DSC) in liquid and spray dried NS.
32777429	7	62	theme	scanning	1109:1116	arg1	calorimetry					1118:1128	differential scanning calorimetry	1096:1128	differential scanning calorimetry (DSC)	1096:1134	Particles crystallinity was confirmed using X-ray powder diffraction (XRPD) and differential scanning calorimetry (DSC) in liquid and spray dried NS.
32777429	4	63	from	solubility	720:729	arg1	100-300 nm					667:676	the range 100-300 nm	657:676	the range 100-300 nm	657:676	NS with uniform PS in the range 100-300 nm, high ζ > ±20 mV, and enhanced saturation solubility were produced.
32777429	4	64	theme	uniform	643:649	arg1	PS					651:652	uniform PS	643:652	uniform PS in the range 100-300 nm	643:676	NS with uniform PS in the range 100-300 nm, high ζ > ±20 mV, and enhanced saturation solubility were produced.
32455682	0	0	theme	Lactobacillus	76:88	arg1	Synbiotics					90:99	Lactobacillus Synbiotics	76:99	Lactobacillus Synbiotics in Broiler Chickens	76:119	Hepatic DNA Methylation in Response to Early Stimulation of Microbiota with Lactobacillus Synbiotics in Broiler Chickens.
32455682	0	1	from	Methylation	12:22	arg1	Response					27:34	Response	27:34	Response to Early Stimulation of Microbiota with Lactobacillus Synbiotics in Broiler Chickens	27:119	Hepatic DNA Methylation in Response to Early Stimulation of Microbiota with Lactobacillus Synbiotics in Broiler Chickens.
32455682	4	2	from	methylation	587:597	arg1	chickens					610:617	broiler chickens	602:617	broiler chickens	602:617	The present study aims to determine the effect of Lactobacillus synbiotics delivered in ovo on the level of hepatic DNA methylation in broiler chickens.
32455682	8	3	theme	genes	919:923	arg1	methylation					899:909	DNA methylation	895:909	DNA methylation of five genes selected on the basis of the transcriptome data	895:971	DNA methylation of five genes selected on the basis of the transcriptome data were analyzed using the qMSP method.
32455682	10	4	theme	expression	1200:1209	arg1	downregulation					1167:1180	the downregulation	1163:1180	the downregulation of metabolic gene expression in the liver mediated by in ovo stimulation	1163:1253	The obtained results confirm that the downregulation of metabolic gene expression in the liver mediated by in ovo stimulation had epigenetic characteristics.
32455682	1	5	theme	promoters	243:251	arg1	islands					227:233	the CpG islands	219:233	the CpG islands of gene promoters	219:251	DNA methylation inhibits DNA transcription by the addition of methyl residues to cysteine within the CpG islands of gene promoters.
32455682	10	6	theme	obtained	1133:1140	arg1	results					1142:1148	The obtained results	1129:1148	The obtained results	1129:1148	The obtained results confirm that the downregulation of metabolic gene expression in the liver mediated by in ovo stimulation had epigenetic characteristics.
32455682	6	7	theme	-Lactobacillus	775:788	arg1	plantarum					790:798	(S2)-Lactobacillus plantarum	771:798	(S2)-Lactobacillus plantarum with RFO	771:807	Bioactive compounds delivered in ovo included (S1)-Lactobacillus salivarius with GOS and (S2)-Lactobacillus plantarum with RFO.
32455682	9	8	theme	Significant	1010:1020	arg1	changes					1022:1028	Significant changes	1010:1028	Significant changes	1010:1028	Significant changes were observed in DNA methylation of genes in liver including ANGPTL4 and NR4A3, after S2 delivery.
32455682	8	9	theme	qMSP	997:1000	arg1	method					1002:1007	the qMSP method	993:1007	the qMSP method	993:1007	DNA methylation of five genes selected on the basis of the transcriptome data were analyzed using the qMSP method.
32455682	9	10	theme	S2	1116:1117	arg1	delivery					1119:1126	S2 delivery	1116:1126	S2 delivery	1116:1126	Significant changes were observed in DNA methylation of genes in liver including ANGPTL4 and NR4A3, after S2 delivery.
32455682	4	11	theme	hepatic	575:581	arg1	methylation					587:597	hepatic DNA methylation	575:597	hepatic DNA methylation in broiler chickens	575:617	The present study aims to determine the effect of Lactobacillus synbiotics delivered in ovo on the level of hepatic DNA methylation in broiler chickens.
32455682	7	12	from	group	864:868	arg1	individuals					842:852	six individuals	838:852	six individuals from each group	838:868	Samples were collected from six individuals from each group on day 42 post-hatching.
32455682	10	13	from	downregulation	1167:1180	arg1	liver					1218:1222	the liver	1214:1222	the liver mediated by in ovo stimulation	1214:1253	The obtained results confirm that the downregulation of metabolic gene expression in the liver mediated by in ovo stimulation had epigenetic characteristics.
32455682	6	14	theme	S2	772:773	arg1	plantarum					790:798	(S2)-Lactobacillus plantarum	771:798	(S2)-Lactobacillus plantarum with RFO	771:807	Bioactive compounds delivered in ovo included (S1)-Lactobacillus salivarius with GOS and (S2)-Lactobacillus plantarum with RFO.
32455682	2	15	theme	methylation	273:283	arg1	process					258:264	The process	254:264	The process of DNA methylation	254:283	The process of DNA methylation can be modulated by environmental factors such as intestinal microbiota.
32455682	4	16	from	level	566:570	arg1	chickens					610:617	broiler chickens	602:617	broiler chickens	602:617	The present study aims to determine the effect of Lactobacillus synbiotics delivered in ovo on the level of hepatic DNA methylation in broiler chickens.
32455682	10	17	contain	had	1255:1257	arg2	characteristics					1270:1284	epigenetic characteristics	1259:1284	epigenetic characteristics	1259:1284	The obtained results confirm that the downregulation of metabolic gene expression in the liver mediated by in ovo stimulation had epigenetic characteristics.
32455682	10	17	contain	had	1255:1257	arg1	downregulation					1167:1180	the downregulation	1163:1180	the downregulation of metabolic gene expression in the liver mediated by in ovo stimulation	1163:1253	The obtained results confirm that the downregulation of metabolic gene expression in the liver mediated by in ovo stimulation had epigenetic characteristics.
32455682	6	18	with	plantarum	790:798	arg1	RFO					805:807	RFO	805:807	RFO	805:807	Bioactive compounds delivered in ovo included (S1)-Lactobacillus salivarius with GOS and (S2)-Lactobacillus plantarum with RFO.
32455682	6	18	with	plantarum	790:798	arg1	GOS					763:765	GOS	763:765	GOS	763:765	Bioactive compounds delivered in ovo included (S1)-Lactobacillus salivarius with GOS and (S2)-Lactobacillus plantarum with RFO.
32455682	2	19	theme	DNA	269:271	arg1	methylation					273:283	DNA methylation	269:283	DNA methylation	269:283	The process of DNA methylation can be modulated by environmental factors such as intestinal microbiota.
32455682	6	20	theme	Bioactive	682:690	arg1	compounds					692:700	Bioactive compounds	682:700	Bioactive compounds delivered in ovo	682:717	Bioactive compounds delivered in ovo included (S1)-Lactobacillus salivarius with GOS and (S2)-Lactobacillus plantarum with RFO.
32455682	0	21	theme	Broiler	104:110	arg1	Chickens					112:119	Broiler Chickens	104:119	Broiler Chickens	104:119	Hepatic DNA Methylation in Response to Early Stimulation of Microbiota with Lactobacillus Synbiotics in Broiler Chickens.
32455682	5	22	dep	In	620:621	arg1	ovo					623:625	ovo	623:625	ovo	623:625	In ovo stimulation was performed on day 12 of egg incubation.
32455682	8	23	theme	transcriptome	954:966	arg1	data					968:971	the transcriptome data	950:971	the transcriptome data	950:971	DNA methylation of five genes selected on the basis of the transcriptome data were analyzed using the qMSP method.
32455682	3	24	theme	in	436:437	arg1	delivery					443:450	in ovo delivery	436:450	in ovo delivery of synbiotics	436:464	In poultry, the composition of the intestinal microbiota can be stimulated by in ovo delivery of synbiotics.
32455682	0	25	theme	DNA	8:10	arg1	Methylation					12:22	Hepatic DNA Methylation	0:22	Hepatic DNA Methylation in Response to Early Stimulation of Microbiota with Lactobacillus Synbiotics in Broiler Chickens.	0:120	Hepatic DNA Methylation in Response to Early Stimulation of Microbiota with Lactobacillus Synbiotics in Broiler Chickens.
32455682	4	26	theme	present	471:477	arg1	study					479:483	The present study	467:483	The present study	467:483	The present study aims to determine the effect of Lactobacillus synbiotics delivered in ovo on the level of hepatic DNA methylation in broiler chickens.
32455682	5	27	theme	In	620:621	arg1	stimulation					627:637	In ovo stimulation	620:637	In ovo stimulation	620:637	In ovo stimulation was performed on day 12 of egg incubation.
32455682	2	28	theme	environmental	305:317	arg1	factors					319:325	environmental factors	305:325	environmental factors such as intestinal microbiota	305:355	The process of DNA methylation can be modulated by environmental factors such as intestinal microbiota.
32455682	2	28	theme	environmental	305:317	arg1	microbiota					346:355	intestinal microbiota	335:355	intestinal microbiota	335:355	The process of DNA methylation can be modulated by environmental factors such as intestinal microbiota.
32455682	9	29	theme	DNA	1047:1049	arg1	methylation					1051:1061	DNA methylation	1047:1061	DNA methylation of genes in liver including ANGPTL4 and NR4A3	1047:1107	Significant changes were observed in DNA methylation of genes in liver including ANGPTL4 and NR4A3, after S2 delivery.
32455682	1	30	theme	methyl	184:189	arg1	residues					191:198	methyl residues	184:198	methyl residues	184:198	DNA methylation inhibits DNA transcription by the addition of methyl residues to cysteine within the CpG islands of gene promoters.
32455682	0	31	theme	Hepatic	0:6	arg1	Methylation					12:22	Hepatic DNA Methylation	0:22	Hepatic DNA Methylation in Response to Early Stimulation of Microbiota with Lactobacillus Synbiotics in Broiler Chickens.	0:120	Hepatic DNA Methylation in Response to Early Stimulation of Microbiota with Lactobacillus Synbiotics in Broiler Chickens.
32455682	7	32	theme	post-hatching	880:892	arg1	day					873:875	day 42 post-hatching	873:892	day 42 post-hatching	873:892	Samples were collected from six individuals from each group on day 42 post-hatching.
32455682	1	33	theme	residues	191:198	arg1	addition					172:179	the addition	168:179	the addition of methyl residues to cysteine within the CpG islands of gene promoters	168:251	DNA methylation inhibits DNA transcription by the addition of methyl residues to cysteine within the CpG islands of gene promoters.
32455682	5	34	theme	incubation	670:679	arg1	day					656:658	day 12	656:661	day 12 of egg incubation	656:679	In ovo stimulation was performed on day 12 of egg incubation.
32455682	3	35	dep	in	436:437	arg1	ovo					439:441	ovo	439:441	ovo	439:441	In poultry, the composition of the intestinal microbiota can be stimulated by in ovo delivery of synbiotics.
32455682	10	36	dep	in	1236:1237	arg1	ovo					1239:1241	ovo	1239:1241	ovo	1239:1241	The obtained results confirm that the downregulation of metabolic gene expression in the liver mediated by in ovo stimulation had epigenetic characteristics.
32455682	10	37	theme	epigenetic	1259:1268	arg1	characteristics					1270:1284	epigenetic characteristics	1259:1284	epigenetic characteristics	1259:1284	The obtained results confirm that the downregulation of metabolic gene expression in the liver mediated by in ovo stimulation had epigenetic characteristics.
32455682	9	38	theme	genes	1066:1070	arg1	methylation					1051:1061	DNA methylation	1047:1061	DNA methylation of genes in liver including ANGPTL4 and NR4A3	1047:1107	Significant changes were observed in DNA methylation of genes in liver including ANGPTL4 and NR4A3, after S2 delivery.
32455682	3	39	theme	synbiotics	455:464	arg1	delivery					443:450	in ovo delivery	436:450	in ovo delivery of synbiotics	436:464	In poultry, the composition of the intestinal microbiota can be stimulated by in ovo delivery of synbiotics.
32455682	3	40	theme	microbiota	404:413	arg1	composition					374:384	the composition	370:384	the composition of the intestinal microbiota	370:413	In poultry, the composition of the intestinal microbiota can be stimulated by in ovo delivery of synbiotics.
32455682	10	41	theme	in	1236:1237	arg1	stimulation					1243:1253	in ovo stimulation	1236:1253	in ovo stimulation	1236:1253	The obtained results confirm that the downregulation of metabolic gene expression in the liver mediated by in ovo stimulation had epigenetic characteristics.
32455682	4	42	from	chickens	610:617	arg1	level					566:570	the level	562:570	the level of hepatic DNA methylation in broiler chickens	562:617	The present study aims to determine the effect of Lactobacillus synbiotics delivered in ovo on the level of hepatic DNA methylation in broiler chickens.
32455682	6	43	with	salivarius	747:756	arg1	RFO					805:807	RFO	805:807	RFO	805:807	Bioactive compounds delivered in ovo included (S1)-Lactobacillus salivarius with GOS and (S2)-Lactobacillus plantarum with RFO.
32455682	6	43	with	salivarius	747:756	arg1	GOS					763:765	GOS	763:765	GOS	763:765	Bioactive compounds delivered in ovo included (S1)-Lactobacillus salivarius with GOS and (S2)-Lactobacillus plantarum with RFO.
32455682	8	44	theme	data	968:971	arg1	basis					941:945	the basis	937:945	the basis of the transcriptome data	937:971	DNA methylation of five genes selected on the basis of the transcriptome data were analyzed using the qMSP method.
32455682	6	45	theme	-Lactobacillus	732:745	arg1	S1					729:730	S1	729:730	S1	729:730	Bioactive compounds delivered in ovo included (S1)-Lactobacillus salivarius with GOS and (S2)-Lactobacillus plantarum with RFO.
32455682	6	45	theme	-Lactobacillus	732:745	arg1	salivarius					747:756	(S1)-Lactobacillus salivarius	728:756	(S1)-Lactobacillus salivarius with GOS	728:765	Bioactive compounds delivered in ovo included (S1)-Lactobacillus salivarius with GOS and (S2)-Lactobacillus plantarum with RFO.
32455682	4	46	from	effect	507:512	arg1	level					566:570	the level	562:570	the level of hepatic DNA methylation in broiler chickens	562:617	The present study aims to determine the effect of Lactobacillus synbiotics delivered in ovo on the level of hepatic DNA methylation in broiler chickens.
32455682	9	47	from	methylation	1051:1061	arg1	liver					1075:1079	liver	1075:1079	liver including ANGPTL4 and NR4A3	1075:1107	Significant changes were observed in DNA methylation of genes in liver including ANGPTL4 and NR4A3, after S2 delivery.
32455682	2	48	theme	intestinal	335:344	arg1	microbiota					346:355	intestinal microbiota	335:355	intestinal microbiota	335:355	The process of DNA methylation can be modulated by environmental factors such as intestinal microbiota.
32455682	0	49	theme	Early	39:43	arg1	Stimulation					45:55	Early Stimulation	39:55	Early Stimulation of Microbiota with Lactobacillus Synbiotics in Broiler Chickens	39:119	Hepatic DNA Methylation in Response to Early Stimulation of Microbiota with Lactobacillus Synbiotics in Broiler Chickens.
32455682	4	50	theme	synbiotics	531:540	arg1	effect					507:512	the effect	503:512	the effect of Lactobacillus synbiotics delivered in ovo on the level of hepatic DNA methylation in broiler chickens	503:617	The present study aims to determine the effect of Lactobacillus synbiotics delivered in ovo on the level of hepatic DNA methylation in broiler chickens.
32455682	3	51	theme	intestinal	393:402	arg1	microbiota					404:413	the intestinal microbiota	389:413	the intestinal microbiota	389:413	In poultry, the composition of the intestinal microbiota can be stimulated by in ovo delivery of synbiotics.
32455682	1	52	theme	DNA	122:124	arg1	methylation					126:136	DNA methylation	122:136	DNA methylation	122:136	DNA methylation inhibits DNA transcription by the addition of methyl residues to cysteine within the CpG islands of gene promoters.
32455682	10	53	theme	gene	1195:1198	arg1	expression					1200:1209	metabolic gene expression	1185:1209	metabolic gene expression	1185:1209	The obtained results confirm that the downregulation of metabolic gene expression in the liver mediated by in ovo stimulation had epigenetic characteristics.
32455682	4	54	theme	Lactobacillus	517:529	arg1	synbiotics					531:540	Lactobacillus synbiotics	517:540	Lactobacillus synbiotics delivered in ovo	517:557	The present study aims to determine the effect of Lactobacillus synbiotics delivered in ovo on the level of hepatic DNA methylation in broiler chickens.
32455682	8	55	theme	DNA	895:897	arg1	methylation					899:909	DNA methylation	895:909	DNA methylation of five genes selected on the basis of the transcriptome data	895:971	DNA methylation of five genes selected on the basis of the transcriptome data were analyzed using the qMSP method.
32455682	10	56	theme	metabolic	1185:1193	arg1	expression					1200:1209	metabolic gene expression	1185:1209	metabolic gene expression	1185:1209	The obtained results confirm that the downregulation of metabolic gene expression in the liver mediated by in ovo stimulation had epigenetic characteristics.
32455682	1	57	theme	CpG	223:225	arg1	islands					227:233	the CpG islands	219:233	the CpG islands of gene promoters	219:251	DNA methylation inhibits DNA transcription by the addition of methyl residues to cysteine within the CpG islands of gene promoters.
32455682	5	58	theme	egg	666:668	arg1	incubation					670:679	egg incubation	666:679	egg incubation	666:679	In ovo stimulation was performed on day 12 of egg incubation.
32455682	0	59	with	Stimulation	45:55	arg1	Synbiotics					90:99	Lactobacillus Synbiotics	76:99	Lactobacillus Synbiotics in Broiler Chickens	76:119	Hepatic DNA Methylation in Response to Early Stimulation of Microbiota with Lactobacillus Synbiotics in Broiler Chickens.
32455682	4	60	theme	DNA	583:585	arg1	methylation					587:597	hepatic DNA methylation	575:597	hepatic DNA methylation in broiler chickens	575:617	The present study aims to determine the effect of Lactobacillus synbiotics delivered in ovo on the level of hepatic DNA methylation in broiler chickens.
32455682	0	61	theme	Microbiota	60:69	arg1	Stimulation					45:55	Early Stimulation	39:55	Early Stimulation of Microbiota with Lactobacillus Synbiotics in Broiler Chickens	39:119	Hepatic DNA Methylation in Response to Early Stimulation of Microbiota with Lactobacillus Synbiotics in Broiler Chickens.
32455682	0	62	from	Synbiotics	90:99	arg1	Chickens					112:119	Broiler Chickens	104:119	Broiler Chickens	104:119	Hepatic DNA Methylation in Response to Early Stimulation of Microbiota with Lactobacillus Synbiotics in Broiler Chickens.
32455682	1	63	theme	DNA	147:149	arg1	transcription					151:163	DNA transcription	147:163	DNA transcription	147:163	DNA methylation inhibits DNA transcription by the addition of methyl residues to cysteine within the CpG islands of gene promoters.
32455682	4	64	theme	broiler	602:608	arg1	chickens					610:617	broiler chickens	602:617	broiler chickens	602:617	The present study aims to determine the effect of Lactobacillus synbiotics delivered in ovo on the level of hepatic DNA methylation in broiler chickens.
32455682	9	65	located	observed	1035:1042	arg2	changes					1022:1028	Significant changes	1010:1028	Significant changes	1010:1028	Significant changes were observed in DNA methylation of genes in liver including ANGPTL4 and NR4A3, after S2 delivery.
32455682	9	65	located	observed	1035:1042	arg1	methylation					1051:1061	DNA methylation	1047:1061	DNA methylation of genes in liver including ANGPTL4 and NR4A3	1047:1107	Significant changes were observed in DNA methylation of genes in liver including ANGPTL4 and NR4A3, after S2 delivery.
32455682	4	66	theme	methylation	587:597	arg1	level					566:570	the level	562:570	the level of hepatic DNA methylation in broiler chickens	562:617	The present study aims to determine the effect of Lactobacillus synbiotics delivered in ovo on the level of hepatic DNA methylation in broiler chickens.
32455682	1	67	theme	gene	238:241	arg1	promoters					243:251	gene promoters	238:251	gene promoters	238:251	DNA methylation inhibits DNA transcription by the addition of methyl residues to cysteine within the CpG islands of gene promoters.
32748000	8	0	theme	YIM	972:974	arg1	B00363T					976:982	the strain YIM B00363T	961:982	the strain YIM B00363T	961:982	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain YIM B00363T belonged to a cluster comprising species of the genus Paenibacillus.
32748000	7	1	theme	G + C	843:847	arg1	%					888:888	50.4 mol%	880:888	50.4 mol%	880:888	The DNA G + C content of the type strain was 50.4 mol%.
32748000	7	1	theme	G + C	843:847	arg1	content					849:855	The DNA G + C content	835:855	The DNA G + C content of the type strain	835:874	The DNA G + C content of the type strain was 50.4 mol%.
32748000	12	2	theme	1.17507T = KCTC	1450:1464	arg1	43184T					1466:1471	= CGMCC 1.17507T = KCTC 43184T	1442:1471	= CGMCC 1.17507T = KCTC 43184T	1442:1471	The type strain is YIM B00363T (= CGMCC 1.17507T = KCTC 43184T).
32748000	12	2	theme	1.17507T = KCTC	1450:1464	arg1	B00363T					1433:1439	YIM B00363T	1429:1439	YIM B00363T (= CGMCC 1.17507T = KCTC 43184T)	1429:1472	The type strain is YIM B00363T (= CGMCC 1.17507T = KCTC 43184T).
32748000	8	3	theme	strain	965:970	arg1	B00363T					976:982	the strain YIM B00363T	961:982	the strain YIM B00363T	961:982	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain YIM B00363T belonged to a cluster comprising species of the genus Paenibacillus.
32748000	8	4	theme	16S	922:924	arg1	sequences					936:944	16S rRNA gene sequences	922:944	16S rRNA gene sequences	922:944	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain YIM B00363T belonged to a cluster comprising species of the genus Paenibacillus.
32748000	2	5	theme	north-west	299:308	arg1	province					289:296	Xinjiang province	280:296	Xinjiang province	280:296	Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach.
32748000	2	5	theme	north-west	299:308	arg1	China					310:314	north-west China	299:314	north-west China	299:314	Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach.
32748000	2	6	from	lake	272:275	arg1	province					289:296	Xinjiang province	280:296	Xinjiang province	280:296	Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach.
32748000	2	6	from	lake	272:275	arg1	China					310:314	north-west China	299:314	north-west China	299:314	Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach.
32748000	10	7	theme	turpanensis	1376:1386	arg1	sp					1388:1389	the name Paenibacillus turpanensis sp	1353:1389	the name Paenibacillus turpanensis sp	1353:1389	On the basis of its phenotypic characteristics and phylogenetic distinctivenes, strain YIM B00363T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus turpanensis sp.
32748000	5	8	theme	cell	613:616	arg1	peptidoglycan					623:635	the cell wall peptidoglycan	609:635	the cell wall peptidoglycan	609:635	The diagnostic diamino acid of the cell wall peptidoglycan was meso-diaminopimelic acid.
32748000	10	9	theme	Paenibacillus	1362:1374	arg1	sp					1388:1389	the name Paenibacillus turpanensis sp	1353:1389	the name Paenibacillus turpanensis sp	1353:1389	On the basis of its phenotypic characteristics and phylogenetic distinctivenes, strain YIM B00363T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus turpanensis sp.
32748000	6	10	theme	unknown	819:825	arg1	lipids					827:832	unknown lipids	819:832	unknown lipids	819:832	The phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids, unidentified glycolipids and unknown lipids.
32748000	5	11	theme	wall	618:621	arg1	peptidoglycan					623:635	the cell wall peptidoglycan	609:635	the cell wall peptidoglycan	609:635	The diagnostic diamino acid of the cell wall peptidoglycan was meso-diaminopimelic acid.
32748000	10	12	theme	name	1357:1360	arg1	sp					1388:1389	the name Paenibacillus turpanensis sp	1353:1389	the name Paenibacillus turpanensis sp	1353:1389	On the basis of its phenotypic characteristics and phylogenetic distinctivenes, strain YIM B00363T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus turpanensis sp.
32748000	8	13	theme	gene	931:934	arg1	sequences					936:944	16S rRNA gene sequences	922:944	16S rRNA gene sequences	922:944	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain YIM B00363T belonged to a cluster comprising species of the genus Paenibacillus.
32748000	1	14	theme	salt	52:55	arg1	lake					57:60	a salt lake	50:60	a salt lake of Turpan city in Xinjiang province, north-west China	50:114	nov., isolated from a salt lake of Turpan city in Xinjiang province, north-west China.
32748000	10	15	theme	phenotypic	1209:1218	arg1	characteristics					1220:1234	its phenotypic characteristics	1205:1234	its phenotypic characteristics	1205:1234	On the basis of its phenotypic characteristics and phylogenetic distinctivenes, strain YIM B00363T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus turpanensis sp.
32748000	2	16	dep	Gram-positive	139:151	arg1	spore-forming					187:199	spore-forming	187:199	spore-forming	187:199	Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach.
32748000	2	16	dep	Gram-positive	139:151	arg1	rod-shaped					175:184	rod-shaped	175:184	rod-shaped	175:184	Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach.
32748000	2	16	dep	Gram-positive	139:151	arg1	non-motile					163:172	non-motile	163:172	non-motile	163:172	Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach.
32748000	2	16	dep	Gram-positive	139:151	arg1	aerobic					154:160	aerobic	154:160	aerobic	154:160	Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach.
32748000	5	17	theme	peptidoglycan	623:635	arg1	acid					601:604	The diagnostic diamino acid	578:604	The diagnostic diamino acid of the cell wall peptidoglycan	578:635	The diagnostic diamino acid of the cell wall peptidoglycan was meso-diaminopimelic acid.
32748000	5	17	theme	peptidoglycan	623:635	arg1	acid					661:664	meso-diaminopimelic acid	641:664	meso-diaminopimelic acid	641:664	The diagnostic diamino acid of the cell wall peptidoglycan was meso-diaminopimelic acid.
32748000	2	18	theme	YIM	124:126	arg1	bacterium					201:209	a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium	137:209	a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium	137:209	Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach.
32748000	2	18	theme	YIM	124:126	arg1	B00363T					128:134	Strain YIM B00363T	117:134	Strain YIM B00363T	117:134	Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach.
32748000	10	19	dep	characteristics	1220:1234	arg1	basis					1196:1200	basis	1196:1200	basis	1196:1200	On the basis of its phenotypic characteristics and phylogenetic distinctivenes, strain YIM B00363T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus turpanensis sp.
32748000	10	19	dep	characteristics	1220:1234	arg1	the					1192:1194	the	1192:1194	the	1192:1194	On the basis of its phenotypic characteristics and phylogenetic distinctivenes, strain YIM B00363T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus turpanensis sp.
32748000	8	20	theme	rRNA	926:929	arg1	sequences					936:944	16S rRNA gene sequences	922:944	16S rRNA gene sequences	922:944	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain YIM B00363T belonged to a cluster comprising species of the genus Paenibacillus.
32748000	2	21	theme	soil	237:240	arg1	samples					242:248	saline soil samples	230:248	saline soil samples collected from a salt lake in Xinjiang province, north-west China	230:314	Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach.
32748000	4	22	theme	fatty	565:569	arg1	anteiso-C15:0					473:485	anteiso-C15:0	473:485	anteiso-C15:0	473:485	The major menaquinone was MK-7; anteiso-C15:0 (53.52%), iso-C15:0 (15.04%) and C16:0 (12.76%) were the predominant cellular fatty acids.
32748000	4	22	theme	fatty	565:569	arg1	acids					571:575	the predominant cellular fatty acids	540:575	the predominant cellular fatty acids	540:575	The major menaquinone was MK-7; anteiso-C15:0 (53.52%), iso-C15:0 (15.04%) and C16:0 (12.76%) were the predominant cellular fatty acids.
32748000	4	22	theme	fatty	565:569	arg1	C16:0					520:524	C16:0	520:524	C16:0 (12.76%)	520:533	The major menaquinone was MK-7; anteiso-C15:0 (53.52%), iso-C15:0 (15.04%) and C16:0 (12.76%) were the predominant cellular fatty acids.
32748000	4	22	theme	fatty	565:569	arg1	iso-C15:0					497:505	iso-C15:0	497:505	iso-C15:0	497:505	The major menaquinone was MK-7; anteiso-C15:0 (53.52%), iso-C15:0 (15.04%) and C16:0 (12.76%) were the predominant cellular fatty acids.
32748000	4	23	theme	predominant	544:554	arg1	anteiso-C15:0					473:485	anteiso-C15:0	473:485	anteiso-C15:0	473:485	The major menaquinone was MK-7; anteiso-C15:0 (53.52%), iso-C15:0 (15.04%) and C16:0 (12.76%) were the predominant cellular fatty acids.
32748000	4	23	theme	predominant	544:554	arg1	acids					571:575	the predominant cellular fatty acids	540:575	the predominant cellular fatty acids	540:575	The major menaquinone was MK-7; anteiso-C15:0 (53.52%), iso-C15:0 (15.04%) and C16:0 (12.76%) were the predominant cellular fatty acids.
32748000	4	23	theme	predominant	544:554	arg1	C16:0					520:524	C16:0	520:524	C16:0 (12.76%)	520:533	The major menaquinone was MK-7; anteiso-C15:0 (53.52%), iso-C15:0 (15.04%) and C16:0 (12.76%) were the predominant cellular fatty acids.
32748000	4	23	theme	predominant	544:554	arg1	iso-C15:0					497:505	iso-C15:0	497:505	iso-C15:0	497:505	The major menaquinone was MK-7; anteiso-C15:0 (53.52%), iso-C15:0 (15.04%) and C16:0 (12.76%) were the predominant cellular fatty acids.
32748000	2	24	theme	Strain	117:122	arg1	bacterium					201:209	a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium	137:209	a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium	137:209	Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach.
32748000	2	24	theme	Strain	117:122	arg1	B00363T					128:134	Strain YIM B00363T	117:134	Strain YIM B00363T	117:134	Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach.
32748000	7	25	theme	50.4 mol	880:887	arg1	%					888:888	50.4 mol%	880:888	50.4 mol%	880:888	The DNA G + C content of the type strain was 50.4 mol%.
32748000	7	25	theme	50.4 mol	880:887	arg1	content					849:855	The DNA G + C content	835:855	The DNA G + C content of the type strain	835:874	The DNA G + C content of the type strain was 50.4 mol%.
32748000	2	26	theme	saline	230:235	arg1	samples					242:248	saline soil samples	230:248	saline soil samples collected from a salt lake in Xinjiang province, north-west China	230:314	Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach.
32748000	0	27	theme	turpanensis	14:24	arg1	sp					26:27	Paenibacillus turpanensis sp	0:27	Paenibacillus turpanensis sp.	0:28	Paenibacillus turpanensis sp.
32748000	1	28	theme	Turpan	65:70	arg1	city					72:75	Turpan city	65:75	Turpan city	65:75	nov., isolated from a salt lake of Turpan city in Xinjiang province, north-west China.
32748000	3	29	theme	optimum	372:378	arg1	temperature					387:397	The optimum growth temperature	368:397	The optimum growth temperature	368:397	The optimum growth temperature was 37 °C and the optimum pH was 7.5-8.0.
32748000	0	30	theme	Paenibacillus	0:12	arg1	sp					26:27	Paenibacillus turpanensis sp	0:27	Paenibacillus turpanensis sp.	0:28	Paenibacillus turpanensis sp.
32748000	9	31	dep	%	1145:1145	arg1	similarities					1161:1172	gene sequence similarities	1147:1172	92.8% gene sequence similarities	1141:1172	The nearest relatives were P. residui MC-246T and P. senegalensis JC66T, with 93.2% and 92.8% gene sequence similarities, respectively.
32748000	12	32	theme	type	1414:1417	arg1	strain					1419:1424	The type strain	1410:1424	The type strain	1410:1424	The type strain is YIM B00363T (= CGMCC 1.17507T = KCTC 43184T).
32748000	12	32	theme	type	1414:1417	arg1	B00363T					1433:1439	YIM B00363T	1429:1439	YIM B00363T (= CGMCC 1.17507T = KCTC 43184T)	1429:1472	The type strain is YIM B00363T (= CGMCC 1.17507T = KCTC 43184T).
32748000	7	33	theme	strain	869:874	arg1	%					888:888	50.4 mol%	880:888	50.4 mol%	880:888	The DNA G + C content of the type strain was 50.4 mol%.
32748000	7	33	theme	strain	869:874	arg1	content					849:855	The DNA G + C content	835:855	The DNA G + C content of the type strain	835:874	The DNA G + C content of the type strain was 50.4 mol%.
32748000	1	34	theme	city	72:75	arg1	lake					57:60	a salt lake	50:60	a salt lake of Turpan city in Xinjiang province, north-west China	50:114	nov., isolated from a salt lake of Turpan city in Xinjiang province, north-west China.
32748000	3	35	theme	growth	380:385	arg1	temperature					387:397	The optimum growth temperature	368:397	The optimum growth temperature	368:397	The optimum growth temperature was 37 °C and the optimum pH was 7.5-8.0.
32748000	5	36	theme	diamino	593:599	arg1	acid					601:604	The diagnostic diamino acid	578:604	The diagnostic diamino acid of the cell wall peptidoglycan	578:635	The diagnostic diamino acid of the cell wall peptidoglycan was meso-diaminopimelic acid.
32748000	5	36	theme	diamino	593:599	arg1	acid					661:664	meso-diaminopimelic acid	641:664	meso-diaminopimelic acid	641:664	The diagnostic diamino acid of the cell wall peptidoglycan was meso-diaminopimelic acid.
32748000	10	37	theme	phylogenetic	1240:1251	arg1	distinctivenes					1253:1266	phylogenetic distinctivenes	1240:1266	phylogenetic distinctivenes	1240:1266	On the basis of its phenotypic characteristics and phylogenetic distinctivenes, strain YIM B00363T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus turpanensis sp.
32748000	9	38	theme	sequence	1152:1159	arg1	similarities					1161:1172	gene sequence similarities	1147:1172	92.8% gene sequence similarities	1141:1172	The nearest relatives were P. residui MC-246T and P. senegalensis JC66T, with 93.2% and 92.8% gene sequence similarities, respectively.
32748000	2	39	theme	salt	267:270	arg1	lake					272:275	a salt lake	265:275	a salt lake in Xinjiang province, north-west China	265:314	Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach.
32748000	9	40	theme	gene	1147:1150	arg1	similarities					1161:1172	gene sequence similarities	1147:1172	92.8% gene sequence similarities	1141:1172	The nearest relatives were P. residui MC-246T and P. senegalensis JC66T, with 93.2% and 92.8% gene sequence similarities, respectively.
32748000	9	41	theme	nearest	1057:1063	arg1	relatives					1065:1073	The nearest relatives	1053:1073	The nearest relatives	1053:1073	The nearest relatives were P. residui MC-246T and P. senegalensis JC66T, with 93.2% and 92.8% gene sequence similarities, respectively.
32748000	9	41	theme	nearest	1057:1063	arg1	MC-246T					1091:1097	MC-246T	1091:1097	MC-246T	1091:1097	The nearest relatives were P. residui MC-246T and P. senegalensis JC66T, with 93.2% and 92.8% gene sequence similarities, respectively.
32748000	3	42	theme	optimum	417:423	arg1	7.5-8.0					432:438	7.5-8.0	432:438	7.5-8.0	432:438	The optimum growth temperature was 37 °C and the optimum pH was 7.5-8.0.
32748000	3	42	theme	optimum	417:423	arg1	pH					425:426	the optimum pH	413:426	the optimum pH	413:426	The optimum growth temperature was 37 °C and the optimum pH was 7.5-8.0.
32748000	10	43	theme	Paenibacillus	1328:1340	arg1	genus					1322:1326	the genus Paenibacillus	1318:1340	the genus Paenibacillus	1318:1340	On the basis of its phenotypic characteristics and phylogenetic distinctivenes, strain YIM B00363T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus turpanensis sp.
32748000	8	44	theme	genus	1032:1036	arg1	species					1017:1023	species	1017:1023	species of the genus Paenibacillus	1017:1050	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain YIM B00363T belonged to a cluster comprising species of the genus Paenibacillus.
32748000	2	45	theme	Gram-positive	139:151	arg1	bacterium					201:209	a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium	137:209	a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium	137:209	Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach.
32748000	2	45	theme	Gram-positive	139:151	arg1	B00363T					128:134	Strain YIM B00363T	117:134	Strain YIM B00363T	117:134	Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach.
32748000	4	46	theme	cellular	556:563	arg1	anteiso-C15:0					473:485	anteiso-C15:0	473:485	anteiso-C15:0	473:485	The major menaquinone was MK-7; anteiso-C15:0 (53.52%), iso-C15:0 (15.04%) and C16:0 (12.76%) were the predominant cellular fatty acids.
32748000	4	46	theme	cellular	556:563	arg1	acids					571:575	the predominant cellular fatty acids	540:575	the predominant cellular fatty acids	540:575	The major menaquinone was MK-7; anteiso-C15:0 (53.52%), iso-C15:0 (15.04%) and C16:0 (12.76%) were the predominant cellular fatty acids.
32748000	4	46	theme	cellular	556:563	arg1	C16:0					520:524	C16:0	520:524	C16:0 (12.76%)	520:533	The major menaquinone was MK-7; anteiso-C15:0 (53.52%), iso-C15:0 (15.04%) and C16:0 (12.76%) were the predominant cellular fatty acids.
32748000	4	46	theme	cellular	556:563	arg1	iso-C15:0					497:505	iso-C15:0	497:505	iso-C15:0	497:505	The major menaquinone was MK-7; anteiso-C15:0 (53.52%), iso-C15:0 (15.04%) and C16:0 (12.76%) were the predominant cellular fatty acids.
32748000	12	47	theme	= CGMCC	1442:1448	arg1	43184T					1466:1471	= CGMCC 1.17507T = KCTC 43184T	1442:1471	= CGMCC 1.17507T = KCTC 43184T	1442:1471	The type strain is YIM B00363T (= CGMCC 1.17507T = KCTC 43184T).
32748000	12	47	theme	= CGMCC	1442:1448	arg1	B00363T					1433:1439	YIM B00363T	1429:1439	YIM B00363T (= CGMCC 1.17507T = KCTC 43184T)	1429:1472	The type strain is YIM B00363T (= CGMCC 1.17507T = KCTC 43184T).
32748000	2	48	theme	polyphasic	347:356	arg1	approach					358:365	a polyphasic approach	345:365	a polyphasic approach	345:365	Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach.
32748000	10	49	theme	YIM	1276:1278	arg1	B00363T					1280:1286	strain YIM B00363T	1269:1286	strain YIM B00363T	1269:1286	On the basis of its phenotypic characteristics and phylogenetic distinctivenes, strain YIM B00363T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus turpanensis sp.
32748000	8	50	theme	Paenibacillus	1038:1050	arg1	genus					1032:1036	the genus Paenibacillus	1028:1050	the genus Paenibacillus	1028:1050	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain YIM B00363T belonged to a cluster comprising species of the genus Paenibacillus.
32748000	6	51	theme	unidentified	790:801	arg1	glycolipids					803:813	unidentified glycolipids	790:813	unidentified glycolipids	790:813	The phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids, unidentified glycolipids and unknown lipids.
32748000	10	52	theme	strain	1269:1274	arg1	B00363T					1280:1286	strain YIM B00363T	1269:1286	strain YIM B00363T	1269:1286	On the basis of its phenotypic characteristics and phylogenetic distinctivenes, strain YIM B00363T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus turpanensis sp.
32748000	12	53	theme	YIM	1429:1431	arg1	43184T					1466:1471	= CGMCC 1.17507T = KCTC 43184T	1442:1471	= CGMCC 1.17507T = KCTC 43184T	1442:1471	The type strain is YIM B00363T (= CGMCC 1.17507T = KCTC 43184T).
32748000	12	53	theme	YIM	1429:1431	arg1	strain					1419:1424	The type strain	1410:1424	The type strain	1410:1424	The type strain is YIM B00363T (= CGMCC 1.17507T = KCTC 43184T).
32748000	12	53	theme	YIM	1429:1431	arg1	B00363T					1433:1439	YIM B00363T	1429:1439	YIM B00363T (= CGMCC 1.17507T = KCTC 43184T)	1429:1472	The type strain is YIM B00363T (= CGMCC 1.17507T = KCTC 43184T).
32748000	10	54	theme	novel	1301:1305	arg1	species					1307:1313	a novel species	1299:1313	a novel species	1299:1313	On the basis of its phenotypic characteristics and phylogenetic distinctivenes, strain YIM B00363T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus turpanensis sp.
32748000	10	55	theme	genus	1322:1326	arg1	species					1307:1313	a novel species	1299:1313	a novel species	1299:1313	On the basis of its phenotypic characteristics and phylogenetic distinctivenes, strain YIM B00363T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus turpanensis sp.
32748000	2	56	theme	Xinjiang	280:287	arg1	province					289:296	Xinjiang province	280:296	Xinjiang province	280:296	Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach.
32748000	2	56	theme	Xinjiang	280:287	arg1	China					310:314	north-west China	299:314	north-west China	299:314	Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach.
32748000	7	57	theme	type	864:867	arg1	strain					869:874	the type strain	860:874	the type strain	860:874	The DNA G + C content of the type strain was 50.4 mol%.
32748000	2	58	attach	isolated	216:223	arg2	B00363T					128:134	Strain YIM B00363T	117:134	Strain YIM B00363T	117:134	Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach.
32748000	2	58	attach	isolated	216:223	arg1	samples					242:248	saline soil samples	230:248	saline soil samples collected from a salt lake in Xinjiang province, north-west China	230:314	Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach.
32748000	2	58	attach	isolated	216:223	arg2	bacterium					201:209	a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium	137:209	a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium	137:209	Strain YIM B00363T, a Gram-positive, aerobic, non-motile, rod-shaped, spore-forming bacterium, was isolated from saline soil samples collected from a salt lake in Xinjiang province, north-west China, and was characterized using a polyphasic approach.
32748000	5	59	theme	diagnostic	582:591	arg1	acid					601:604	The diagnostic diamino acid	578:604	The diagnostic diamino acid of the cell wall peptidoglycan	578:635	The diagnostic diamino acid of the cell wall peptidoglycan was meso-diaminopimelic acid.
32748000	5	59	theme	diagnostic	582:591	arg1	acid					661:664	meso-diaminopimelic acid	641:664	meso-diaminopimelic acid	641:664	The diagnostic diamino acid of the cell wall peptidoglycan was meso-diaminopimelic acid.
32748000	8	60	theme	Phylogenetic	891:902	arg1	analysis					904:911	Phylogenetic analysis	891:911	Phylogenetic analysis based on 16S rRNA gene sequences	891:944	Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain YIM B00363T belonged to a cluster comprising species of the genus Paenibacillus.
32748000	5	61	theme	meso-diaminopimelic	641:659	arg1	acid					661:664	meso-diaminopimelic acid	641:664	meso-diaminopimelic acid	641:664	The diagnostic diamino acid of the cell wall peptidoglycan was meso-diaminopimelic acid.
32748000	5	61	theme	meso-diaminopimelic	641:659	arg1	acid					601:604	The diagnostic diamino acid	578:604	The diagnostic diamino acid of the cell wall peptidoglycan	578:635	The diagnostic diamino acid of the cell wall peptidoglycan was meso-diaminopimelic acid.
32748000	6	62	theme	unidentified	762:773	arg1	phospholipids					775:787	unidentified phospholipids	762:787	unidentified phospholipids	762:787	The phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids, unidentified glycolipids and unknown lipids.
32748000	7	63	theme	DNA	839:841	arg1	%					888:888	50.4 mol%	880:888	50.4 mol%	880:888	The DNA G + C content of the type strain was 50.4 mol%.
32748000	7	63	theme	DNA	839:841	arg1	content					849:855	The DNA G + C content	835:855	The DNA G + C content of the type strain	835:874	The DNA G + C content of the type strain was 50.4 mol%.
32748000	4	64	theme	major	445:449	arg1	MK-7					467:470	MK-7	467:470	MK-7	467:470	The major menaquinone was MK-7; anteiso-C15:0 (53.52%), iso-C15:0 (15.04%) and C16:0 (12.76%) were the predominant cellular fatty acids.
32748000	4	64	theme	major	445:449	arg1	menaquinone					451:461	The major menaquinone	441:461	The major menaquinone	441:461	The major menaquinone was MK-7; anteiso-C15:0 (53.52%), iso-C15:0 (15.04%) and C16:0 (12.76%) were the predominant cellular fatty acids.
32748000	1	65	from	lake	57:60	arg1	north-west					99:108	north-west	99:108	north-west	99:108	nov., isolated from a salt lake of Turpan city in Xinjiang province, north-west China.
32748000	1	65	from	lake	57:60	arg1	China					110:114	China	110:114	China	110:114	nov., isolated from a salt lake of Turpan city in Xinjiang province, north-west China.
33535571	7	0	theme	healthy	1584:1590	arg1	donors					1592:1597	the healthy donors	1580:1597	the healthy donors (area under the curve 0.93-1.00)	1580:1630	Notably, receiver operating characteristic analyses demonstrated that a single N-glycan isomer could effectively stratify each of the four bacteremic patient groups from the healthy donors (area under the curve 0.93-1.00).
33535571	4	1	theme	N-glycan	810:817	arg1	isomers					819:825	62 N-glycan isomers	807:825	62 N-glycan isomers spanning 41 glycan compositions primarily comprising complex-type core fucosylated, bisecting N-acetylglucosamine (GlcNAc), and α2,3-/α2,6-sialylated structures	807:986	In total, 62 N-glycan isomers spanning 41 glycan compositions primarily comprising complex-type core fucosylated, bisecting N-acetylglucosamine (GlcNAc), and α2,3-/α2,6-sialylated structures were profiled across all samples using label-free quantitation.
33535571	1	2	theme	long-term	166:174	arg1	morbidity					176:184	long-term morbidity	166:184	long-term morbidity	166:184	Bacteremia-i.e., the presence of pathogens in the blood stream-is associated with long-term morbidity and is a potential precursor condition to life-threatening sepsis.
33535571	4	3	theme	label-free	1027:1036	arg1	quantitation					1038:1049	label-free quantitation	1027:1049	label-free quantitation	1027:1049	In total, 62 N-glycan isomers spanning 41 glycan compositions primarily comprising complex-type core fucosylated, bisecting N-acetylglucosamine (GlcNAc), and α2,3-/α2,6-sialylated structures were profiled across all samples using label-free quantitation.
33535571	7	4	dep	donors	1592:1597	arg1	area					1600:1603	area	1600:1603	area under the curve 0.93-1.00	1600:1629	Notably, receiver operating characteristic analyses demonstrated that a single N-glycan isomer could effectively stratify each of the four bacteremic patient groups from the healthy donors (area under the curve 0.93-1.00).
33535571	7	5	theme	characteristic	1438:1451	arg1	analyses					1453:1460	characteristic analyses	1438:1460	characteristic analyses	1438:1460	Notably, receiver operating characteristic analyses demonstrated that a single N-glycan isomer could effectively stratify each of the four bacteremic patient groups from the healthy donors (area under the curve 0.93-1.00).
33535571	5	6	theme	serum	1141:1145	arg1	data					1157:1160	the serum N-glycome data	1137:1160	the serum N-glycome data	1137:1160	Excitingly, unsupervised hierarchical clustering and principal component analysis of the serum N-glycome data accurately separated the patient groups.
33535571	3	7	theme	liquid	548:553	arg1	spectrometry					582:593	porous graphitized carbon liquid chromatography tandem mass spectrometry	522:593	porous graphitized carbon liquid chromatography tandem mass spectrometry	522:593	Herein, we tested the potential of quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39).
33535571	6	8	theme	reduced	1351:1357	arg1	sialylation					1359:1369	reduced sialylation	1351:1369	reduced sialylation	1351:1369	P. aeruginosa-infected patients displayed prominent N-glycome aberrations involving elevated levels of fucosylation and bisecting GlcNAcylation and reduced sialylation relative to other bacteremic patients.
33535571	4	9	theme	bisecting	911:919	arg1	N-acetylglucosamine					921:939	bisecting N-acetylglucosamine	911:939	bisecting N-acetylglucosamine (GlcNAc)	911:948	In total, 62 N-glycan isomers spanning 41 glycan compositions primarily comprising complex-type core fucosylated, bisecting N-acetylglucosamine (GlcNAc), and α2,3-/α2,6-sialylated structures were profiled across all samples using label-free quantitation.
33535571	4	9	theme	bisecting	911:919	arg1	GlcNAc					942:947	GlcNAc	942:947	GlcNAc	942:947	In total, 62 N-glycan isomers spanning 41 glycan compositions primarily comprising complex-type core fucosylated, bisecting N-acetylglucosamine (GlcNAc), and α2,3-/α2,6-sialylated structures were profiled across all samples using label-free quantitation.
33535571	5	10	theme	N-glycome	1147:1155	arg1	data					1157:1160	the serum N-glycome data	1137:1160	the serum N-glycome data	1137:1160	Excitingly, unsupervised hierarchical clustering and principal component analysis of the serum N-glycome data accurately separated the patient groups.
33535571	6	11	theme	GlcNAcylation	1333:1345	arg1	levels					1296:1301	elevated levels	1287:1301	elevated levels of fucosylation and bisecting GlcNAcylation and reduced sialylation relative to other bacteremic patients	1287:1407	P. aeruginosa-infected patients displayed prominent N-glycome aberrations involving elevated levels of fucosylation and bisecting GlcNAcylation and reduced sialylation relative to other bacteremic patients.
33535571	2	12	theme	patient	316:322	arg1	mortality					324:332	patient mortality	316:332	patient mortality	316:332	Timely detection of bacteremia is therefore critical to reduce patient mortality, but existing methods lack precision, speed, and sensitivity to effectively stratify bacteremic patients.
33535571	7	13	theme	N-glycan	1489:1496	arg1	isomer					1498:1503	a single N-glycan isomer	1480:1503	a single N-glycan isomer	1480:1503	Notably, receiver operating characteristic analyses demonstrated that a single N-glycan isomer could effectively stratify each of the four bacteremic patient groups from the healthy donors (area under the curve 0.93-1.00).
33535571	8	14	theme	markers	1726:1732	arg1	class					1696:1700	a new hitherto unexplored class	1670:1700	a new hitherto unexplored class of potential diagnostic markers for bloodstream infections	1670:1759	Thus, the serum N-glycome represents a new hitherto unexplored class of potential diagnostic markers for bloodstream infections.
33535571	7	15	theme	bacteremic	1549:1558	arg1	groups					1568:1573	the four bacteremic patient groups	1540:1573	the four bacteremic patient groups	1540:1573	Notably, receiver operating characteristic analyses demonstrated that a single N-glycan isomer could effectively stratify each of the four bacteremic patient groups from the healthy donors (area under the curve 0.93-1.00).
33535571	6	16	theme	bisecting	1323:1331	arg1	GlcNAcylation					1333:1345	bisecting GlcNAcylation	1323:1345	bisecting GlcNAcylation	1323:1345	P. aeruginosa-infected patients displayed prominent N-glycome aberrations involving elevated levels of fucosylation and bisecting GlcNAcylation and reduced sialylation relative to other bacteremic patients.
33535571	3	17	theme	carbon	541:546	arg1	spectrometry					582:593	porous graphitized carbon liquid chromatography tandem mass spectrometry	522:593	porous graphitized carbon liquid chromatography tandem mass spectrometry	522:593	Herein, we tested the potential of quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39).
33535571	3	18	theme	=	790:790	arg1	donors					780:785	healthy donors	772:785	healthy donors (n = 39)	772:794	Herein, we tested the potential of quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39).
33535571	3	18	theme	=	790:790	arg1	n					788:788	n = 39	788:793	n = 39	788:793	Herein, we tested the potential of quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39).
33535571	5	19	theme	principal	1105:1113	arg1	analysis					1125:1132	principal component analysis	1105:1132	principal component analysis	1105:1132	Excitingly, unsupervised hierarchical clustering and principal component analysis of the serum N-glycome data accurately separated the patient groups.
33535571	3	20	theme	mass	577:580	arg1	spectrometry					582:593	porous graphitized carbon liquid chromatography tandem mass spectrometry	522:593	porous graphitized carbon liquid chromatography tandem mass spectrometry	522:593	Herein, we tested the potential of quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39).
33535571	2	21	theme	bacteremic	419:428	arg1	patients					430:437	bacteremic patients	419:437	bacteremic patients	419:437	Timely detection of bacteremia is therefore critical to reduce patient mortality, but existing methods lack precision, speed, and sensitivity to effectively stratify bacteremic patients.
33535571	8	22	theme	diagnostic	1715:1724	arg1	markers					1726:1732	potential diagnostic markers	1705:1732	potential diagnostic markers for bloodstream infections	1705:1759	Thus, the serum N-glycome represents a new hitherto unexplored class of potential diagnostic markers for bloodstream infections.
33535571	3	23	theme	=	726:726	arg1	n					724:724	n = 5	724:728	n = 5	724:728	Herein, we tested the potential of quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39).
33535571	3	23	theme	=	726:726	arg1	aeruginosa					712:721	Pseudomonas aeruginosa	700:721	Pseudomonas aeruginosa (n = 5)	700:729	Herein, we tested the potential of quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39).
33535571	6	24	theme	fucosylation	1306:1317	arg1	levels					1296:1301	elevated levels	1287:1301	elevated levels of fucosylation and bisecting GlcNAcylation and reduced sialylation relative to other bacteremic patients	1287:1407	P. aeruginosa-infected patients displayed prominent N-glycome aberrations involving elevated levels of fucosylation and bisecting GlcNAcylation and reduced sialylation relative to other bacteremic patients.
33535571	8	25	theme	unexplored	1685:1694	arg1	class					1696:1700	a new hitherto unexplored class	1670:1700	a new hitherto unexplored class of potential diagnostic markers for bloodstream infections	1670:1759	Thus, the serum N-glycome represents a new hitherto unexplored class of potential diagnostic markers for bloodstream infections.
33535571	2	26	theme	existing	339:346	arg1	methods					348:354	existing methods	339:354	existing methods	339:354	Timely detection of bacteremia is therefore critical to reduce patient mortality, but existing methods lack precision, speed, and sensitivity to effectively stratify bacteremic patients.
33535571	7	27	theme	patient	1560:1566	arg1	groups					1568:1573	the four bacteremic patient groups	1540:1573	the four bacteremic patient groups	1540:1573	Notably, receiver operating characteristic analyses demonstrated that a single N-glycan isomer could effectively stratify each of the four bacteremic patient groups from the healthy donors (area under the curve 0.93-1.00).
33535571	0	28	theme	Serum	0:4	arg1	N-Glycomics					6:16	Serum N-Glycomics	0:16	Serum N-Glycomics	0:16	Serum N-Glycomics Stratifies Bacteremic Patients Infected with Different Pathogens.
33535571	5	29	theme	patient	1187:1193	arg1	groups					1195:1200	the patient groups	1183:1200	the patient groups	1183:1200	Excitingly, unsupervised hierarchical clustering and principal component analysis of the serum N-glycome data accurately separated the patient groups.
33535571	1	30	theme	potential	195:203	arg1	condition					215:223	a potential precursor condition	193:223	a potential precursor condition to life-threatening sepsis	193:250	Bacteremia-i.e., the presence of pathogens in the blood stream-is associated with long-term morbidity and is a potential precursor condition to life-threatening sepsis.
33535571	0	31	theme	Bacteremic	29:38	arg1	Patients					40:47	Bacteremic Patients	29:47	Bacteremic Patients Infected with Different Pathogens	29:81	Serum N-Glycomics Stratifies Bacteremic Patients Infected with Different Pathogens.
33535571	3	32	theme	=	661:661	arg1	n					659:659	n = 11	659:664	n = 11	659:664	Herein, we tested the potential of quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39).
33535571	3	32	theme	=	661:661	arg1	coli					653:656	Escherichia coli	641:656	Escherichia coli (n = 11)	641:665	Herein, we tested the potential of quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39).
33535571	4	33	theme	complex-type	880:891	arg1	fucosylated					898:908	complex-type core fucosylated	880:908	complex-type core fucosylated	880:908	In total, 62 N-glycan isomers spanning 41 glycan compositions primarily comprising complex-type core fucosylated, bisecting N-acetylglucosamine (GlcNAc), and α2,3-/α2,6-sialylated structures were profiled across all samples using label-free quantitation.
33535571	8	34	theme	serum	1643:1647	arg1	N-glycome					1649:1657	the serum N-glycome	1639:1657	the serum N-glycome	1639:1657	Thus, the serum N-glycome represents a new hitherto unexplored class of potential diagnostic markers for bloodstream infections.
33535571	3	35	theme	bacteremic	607:616	arg1	patients					618:625	bacteremic patients	607:625	bacteremic patients	607:625	Herein, we tested the potential of quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39).
33535571	1	36	theme	precursor	205:213	arg1	condition					215:223	a potential precursor condition	193:223	a potential precursor condition to life-threatening sepsis	193:250	Bacteremia-i.e., the presence of pathogens in the blood stream-is associated with long-term morbidity and is a potential precursor condition to life-threatening sepsis.
33535571	1	37	theme	pathogens	117:125	arg1	presence					105:112	the presence	101:112	the presence of pathogens in the blood stream-is	101:148	Bacteremia-i.e., the presence of pathogens in the blood stream-is associated with long-term morbidity and is a potential precursor condition to life-threatening sepsis.
33535571	3	38	theme	N-glycomics	494:504	arg1	potential					462:470	the potential	458:470	the potential of quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39)	458:794	Herein, we tested the potential of quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39).
33535571	5	39	theme	unsupervised	1064:1075	arg1	clustering					1090:1099	unsupervised hierarchical clustering	1064:1099	unsupervised hierarchical clustering	1064:1099	Excitingly, unsupervised hierarchical clustering and principal component analysis of the serum N-glycome data accurately separated the patient groups.
33535571	6	40	theme	elevated	1287:1294	arg1	levels					1296:1301	elevated levels	1287:1301	elevated levels of fucosylation and bisecting GlcNAcylation and reduced sialylation relative to other bacteremic patients	1287:1407	P. aeruginosa-infected patients displayed prominent N-glycome aberrations involving elevated levels of fucosylation and bisecting GlcNAcylation and reduced sialylation relative to other bacteremic patients.
33535571	4	41	theme	α2,3-/α2,6-sialylated	955:975	arg1	structures					977:986	α2,3-/α2,6-sialylated structures	955:986	α2,3-/α2,6-sialylated structures	955:986	In total, 62 N-glycan isomers spanning 41 glycan compositions primarily comprising complex-type core fucosylated, bisecting N-acetylglucosamine (GlcNAc), and α2,3-/α2,6-sialylated structures were profiled across all samples using label-free quantitation.
33535571	1	42	attach	presence	105:112	arg2	pathogens					117:125	pathogens	117:125	pathogens	117:125	Bacteremia-i.e., the presence of pathogens in the blood stream-is associated with long-term morbidity and is a potential precursor condition to life-threatening sepsis.
33535571	1	42	attach	presence	105:112	arg1	stream-is					140:148	the blood stream-is	130:148	the blood stream-is	130:148	Bacteremia-i.e., the presence of pathogens in the blood stream-is associated with long-term morbidity and is a potential precursor condition to life-threatening sepsis.
33535571	4	43	theme	core	893:896	arg1	fucosylated					898:908	complex-type core fucosylated	880:908	complex-type core fucosylated	880:908	In total, 62 N-glycan isomers spanning 41 glycan compositions primarily comprising complex-type core fucosylated, bisecting N-acetylglucosamine (GlcNAc), and α2,3-/α2,6-sialylated structures were profiled across all samples using label-free quantitation.
33535571	5	44	theme	hierarchical	1077:1088	arg1	clustering					1090:1099	unsupervised hierarchical clustering	1064:1099	unsupervised hierarchical clustering	1064:1099	Excitingly, unsupervised hierarchical clustering and principal component analysis of the serum N-glycome data accurately separated the patient groups.
33535571	2	45	theme	Timely	253:258	arg1	detection					260:268	Timely detection	253:268	Timely detection of bacteremia	253:282	Timely detection of bacteremia is therefore critical to reduce patient mortality, but existing methods lack precision, speed, and sensitivity to effectively stratify bacteremic patients.
33535571	1	46	theme	life-threatening	228:243	arg1	sepsis					245:250	life-threatening sepsis	228:250	life-threatening sepsis	228:250	Bacteremia-i.e., the presence of pathogens in the blood stream-is associated with long-term morbidity and is a potential precursor condition to life-threatening sepsis.
33535571	0	47	theme	Different	63:71	arg1	Pathogens					73:81	Different Pathogens	63:81	Different Pathogens	63:81	Serum N-Glycomics Stratifies Bacteremic Patients Infected with Different Pathogens.
33535571	5	48	theme	data	1157:1160	arg1	analysis					1125:1132	principal component analysis	1105:1132	principal component analysis	1105:1132	Excitingly, unsupervised hierarchical clustering and principal component analysis of the serum N-glycome data accurately separated the patient groups.
33535571	5	48	theme	data	1157:1160	arg1	clustering					1090:1099	unsupervised hierarchical clustering	1064:1099	unsupervised hierarchical clustering	1064:1099	Excitingly, unsupervised hierarchical clustering and principal component analysis of the serum N-glycome data accurately separated the patient groups.
33535571	4	49	theme	glycan	839:844	arg1	compositions					846:857	41 glycan compositions	836:857	41 glycan compositions primarily comprising complex-type core fucosylated	836:908	In total, 62 N-glycan isomers spanning 41 glycan compositions primarily comprising complex-type core fucosylated, bisecting N-acetylglucosamine (GlcNAc), and α2,3-/α2,6-sialylated structures were profiled across all samples using label-free quantitation.
33535571	5	50	theme	component	1115:1123	arg1	analysis					1125:1132	principal component analysis	1105:1132	principal component analysis	1105:1132	Excitingly, unsupervised hierarchical clustering and principal component analysis of the serum N-glycome data accurately separated the patient groups.
33535571	3	51	theme	porous	522:527	arg1	spectrometry					582:593	porous graphitized carbon liquid chromatography tandem mass spectrometry	522:593	porous graphitized carbon liquid chromatography tandem mass spectrometry	522:593	Herein, we tested the potential of quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39).
33535571	6	52	theme	bacteremic	1389:1398	arg1	patients					1400:1407	other bacteremic patients	1383:1407	other bacteremic patients	1383:1407	P. aeruginosa-infected patients displayed prominent N-glycome aberrations involving elevated levels of fucosylation and bisecting GlcNAcylation and reduced sialylation relative to other bacteremic patients.
33535571	6	53	theme	N-glycome	1255:1263	arg1	aberrations					1265:1275	prominent N-glycome aberrations	1245:1275	prominent N-glycome aberrations involving elevated levels of fucosylation and bisecting GlcNAcylation and reduced sialylation relative to other bacteremic patients	1245:1407	P. aeruginosa-infected patients displayed prominent N-glycome aberrations involving elevated levels of fucosylation and bisecting GlcNAcylation and reduced sialylation relative to other bacteremic patients.
33535571	3	54	theme	graphitized	529:539	arg1	spectrometry					582:593	porous graphitized carbon liquid chromatography tandem mass spectrometry	522:593	porous graphitized carbon liquid chromatography tandem mass spectrometry	522:593	Herein, we tested the potential of quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39).
33535571	8	55	theme	bloodstream	1738:1748	arg1	infections					1750:1759	bloodstream infections	1738:1759	bloodstream infections	1738:1759	Thus, the serum N-glycome represents a new hitherto unexplored class of potential diagnostic markers for bloodstream infections.
33535571	3	56	theme	=	762:762	arg1	viridans					750:757	Streptococcus viridans	736:757	Streptococcus viridans (n = 5)	736:765	Herein, we tested the potential of quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39).
33535571	3	56	theme	=	762:762	arg1	n					760:760	n = 5	760:764	n = 5	760:764	Herein, we tested the potential of quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39).
33535571	6	57	theme	relative	1371:1378	arg1	levels					1296:1301	elevated levels	1287:1301	elevated levels of fucosylation and bisecting GlcNAcylation and reduced sialylation relative to other bacteremic patients	1287:1407	P. aeruginosa-infected patients displayed prominent N-glycome aberrations involving elevated levels of fucosylation and bisecting GlcNAcylation and reduced sialylation relative to other bacteremic patients.
33535571	6	58	theme	prominent	1245:1253	arg1	aberrations					1265:1275	prominent N-glycome aberrations	1245:1275	prominent N-glycome aberrations involving elevated levels of fucosylation and bisecting GlcNAcylation and reduced sialylation relative to other bacteremic patients	1245:1407	P. aeruginosa-infected patients displayed prominent N-glycome aberrations involving elevated levels of fucosylation and bisecting GlcNAcylation and reduced sialylation relative to other bacteremic patients.
33535571	4	59	gly	α2,3-/α2,6-sialylated	955:975	arg1	structures					977:986	α2,3-/α2,6-sialylated structures	955:986	α2,3-/α2,6-sialylated structures	955:986	In total, 62 N-glycan isomers spanning 41 glycan compositions primarily comprising complex-type core fucosylated, bisecting N-acetylglucosamine (GlcNAc), and α2,3-/α2,6-sialylated structures were profiled across all samples using label-free quantitation.
33535571	1	60	theme	blood	134:138	arg1	stream-is					140:148	the blood stream-is	130:148	the blood stream-is	130:148	Bacteremia-i.e., the presence of pathogens in the blood stream-is associated with long-term morbidity and is a potential precursor condition to life-threatening sepsis.
33535571	7	61	theme	single	1482:1487	arg1	isomer					1498:1503	a single N-glycan isomer	1480:1503	a single N-glycan isomer	1480:1503	Notably, receiver operating characteristic analyses demonstrated that a single N-glycan isomer could effectively stratify each of the four bacteremic patient groups from the healthy donors (area under the curve 0.93-1.00).
33535571	6	62	theme	sialylation	1359:1369	arg1	levels					1296:1301	elevated levels	1287:1301	elevated levels of fucosylation and bisecting GlcNAcylation and reduced sialylation relative to other bacteremic patients	1287:1407	P. aeruginosa-infected patients displayed prominent N-glycome aberrations involving elevated levels of fucosylation and bisecting GlcNAcylation and reduced sialylation relative to other bacteremic patients.
33535571	3	63	theme	=	693:693	arg1	n					691:691	n = 11	691:696	n = 11	691:696	Herein, we tested the potential of quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39).
33535571	3	63	theme	=	693:693	arg1	aureus					683:688	Staphylococcus aureus	668:688	Staphylococcus aureus (n = 11)	668:697	Herein, we tested the potential of quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39).
33535571	6	64	theme	P.	1203:1204	arg1	patients					1226:1233	P. aeruginosa-infected patients	1203:1233	P. aeruginosa-infected patients	1203:1233	P. aeruginosa-infected patients displayed prominent N-glycome aberrations involving elevated levels of fucosylation and bisecting GlcNAcylation and reduced sialylation relative to other bacteremic patients.
33535571	3	65	dep	stratify	598:605	arg1	infected					627:634	infected	627:634	infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39)	627:794	Herein, we tested the potential of quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39).
33535571	8	66	theme	new	1672:1674	arg1	class					1696:1700	a new hitherto unexplored class	1670:1700	a new hitherto unexplored class of potential diagnostic markers for bloodstream infections	1670:1759	Thus, the serum N-glycome represents a new hitherto unexplored class of potential diagnostic markers for bloodstream infections.
33535571	3	67	theme	quantitative	475:486	arg1	N-glycomics					494:504	quantitative serum N-glycomics	475:504	quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39)	475:794	Herein, we tested the potential of quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39).
33535571	2	68	theme	bacteremia	273:282	arg1	detection					260:268	Timely detection	253:268	Timely detection of bacteremia	253:282	Timely detection of bacteremia is therefore critical to reduce patient mortality, but existing methods lack precision, speed, and sensitivity to effectively stratify bacteremic patients.
33535571	3	69	theme	serum	488:492	arg1	N-glycomics					494:504	quantitative serum N-glycomics	475:504	quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39)	475:794	Herein, we tested the potential of quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39).
33535571	3	70	theme	chromatography	555:568	arg1	spectrometry					582:593	porous graphitized carbon liquid chromatography tandem mass spectrometry	522:593	porous graphitized carbon liquid chromatography tandem mass spectrometry	522:593	Herein, we tested the potential of quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39).
33535571	1	71	from	presence	105:112	arg1	stream-is					140:148	the blood stream-is	130:148	the blood stream-is	130:148	Bacteremia-i.e., the presence of pathogens in the blood stream-is associated with long-term morbidity and is a potential precursor condition to life-threatening sepsis.
33535571	6	72	theme	other	1383:1387	arg1	patients					1400:1407	other bacteremic patients	1383:1407	other bacteremic patients	1383:1407	P. aeruginosa-infected patients displayed prominent N-glycome aberrations involving elevated levels of fucosylation and bisecting GlcNAcylation and reduced sialylation relative to other bacteremic patients.
33535571	6	73	theme	aeruginosa-infected	1206:1224	arg1	patients					1226:1233	P. aeruginosa-infected patients	1203:1233	P. aeruginosa-infected patients	1203:1233	P. aeruginosa-infected patients displayed prominent N-glycome aberrations involving elevated levels of fucosylation and bisecting GlcNAcylation and reduced sialylation relative to other bacteremic patients.
33535571	3	74	theme	tandem	570:575	arg1	spectrometry					582:593	porous graphitized carbon liquid chromatography tandem mass spectrometry	522:593	porous graphitized carbon liquid chromatography tandem mass spectrometry	522:593	Herein, we tested the potential of quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39).
33535571	8	75	theme	potential	1705:1713	arg1	markers					1726:1732	potential diagnostic markers	1705:1732	potential diagnostic markers for bloodstream infections	1705:1759	Thus, the serum N-glycome represents a new hitherto unexplored class of potential diagnostic markers for bloodstream infections.
33535571	3	76	theme	healthy	772:778	arg1	donors					780:785	healthy donors	772:785	healthy donors (n = 39)	772:794	Herein, we tested the potential of quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39).
33535571	3	76	theme	healthy	772:778	arg1	n					788:788	n = 39	788:793	n = 39	788:793	Herein, we tested the potential of quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from healthy donors (n = 39).
33501867	2	0	theme	dressings	360:368	arg1	composition					339:349	the composition	335:349	the composition of wound dressings based on lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate (PMB)	335:467	To develop the lidocaine membranes of enhanced local anesthetic effects, we have designed to establish the composition of wound dressings based on lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate (PMB).
33501867	7	1	theme	in vivo	879:885	arg1	healing					887:893	in vivo healing	879:893	in vivo healing of skin wounds formed in mouse models over 16 days	879:944	Moreover, we examined in vivo healing of skin wounds formed in mouse models over 16 days.
33501867	9	2	theme	tissue	1178:1183	arg1	repair					1185:1190	tissue repair	1178:1190	tissue repair	1178:1190	These findings indicate that LCH-CS/PMB-based bandaging materials could be a potential innovative biomaterial for tissue repair and regeneration for wound healing applications in an animal model.
33501867	2	3	theme	wound	354:358	arg1	dressings					360:368	wound dressings	354:368	wound dressings based on lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate (PMB)	354:467	To develop the lidocaine membranes of enhanced local anesthetic effects, we have designed to establish the composition of wound dressings based on lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate (PMB).
33501867	0	4	theme	tissue	122:127	arg1	regeneration					129:140	in vivo tissue regeneration	114:140	in vivo tissue regeneration	114:140	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles delivery for wound healing: in vitro and in vivo tissue regeneration.
33501867	0	4	theme	tissue	122:127	arg1	nanoparticles					59:71	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles	0:71	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles delivery for wound healing: in vitro and in vivo tissue regeneration.	0:141	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles delivery for wound healing: in vitro and in vivo tissue regeneration.
33501867	8	5	theme	untreated	966:974	arg1	wounds					976:981	the untreated wounds	962:981	the untreated wounds	962:981	In contrast to the untreated wounds, rapid healing was perceived in the LCH-CS/PMB-treated wound with less damaging.
33501867	0	6	theme	in vivo	114:120	arg1	regeneration					129:140	in vivo tissue regeneration	114:140	in vivo tissue regeneration	114:140	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles delivery for wound healing: in vitro and in vivo tissue regeneration.
33501867	0	6	theme	in vivo	114:120	arg1	nanoparticles					59:71	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles	0:71	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles delivery for wound healing: in vitro and in vivo tissue regeneration.	0:141	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles delivery for wound healing: in vitro and in vivo tissue regeneration.
33501867	6	7	theme	antibacterial	773:785	arg1	efficacy					787:794	antibacterial efficacy	773:794	antibacterial efficacy	773:794	Additionally, antibacterial efficacy was developed for gram-positive and negative microorganisms.
33501867	2	8	theme	effects	296:302	arg1	membranes					257:265	the lidocaine membranes	243:265	the lidocaine membranes of enhanced local anesthetic effects	243:302	To develop the lidocaine membranes of enhanced local anesthetic effects, we have designed to establish the composition of wound dressings based on lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate (PMB).
33501867	2	9	dep	-loaded	421:427	arg1	LCH					399:401	LCH	399:401	LCH	399:401	To develop the lidocaine membranes of enhanced local anesthetic effects, we have designed to establish the composition of wound dressings based on lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate (PMB).
33501867	2	9	dep	-loaded	421:427	arg1	chloride					389:396	lidocaine chloride	379:396	lidocaine chloride (LCH)	379:402	To develop the lidocaine membranes of enhanced local anesthetic effects, we have designed to establish the composition of wound dressings based on lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate (PMB).
33501867	2	9	dep	-loaded	421:427	arg1	drug					416:419	anesthetic drug	405:419	anesthetic drug	405:419	To develop the lidocaine membranes of enhanced local anesthetic effects, we have designed to establish the composition of wound dressings based on lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate (PMB).
33501867	9	10	theme	regeneration	1196:1207	arg1	applications					1227:1238	tissue repair and regeneration for wound healing applications	1178:1238	tissue repair and regeneration for wound healing applications	1178:1238	These findings indicate that LCH-CS/PMB-based bandaging materials could be a potential innovative biomaterial for tissue repair and regeneration for wound healing applications in an animal model.
33501867	2	11	theme	anesthetic	285:294	arg1	effects					296:302	enhanced local anesthetic effects	270:302	enhanced local anesthetic effects	270:302	To develop the lidocaine membranes of enhanced local anesthetic effects, we have designed to establish the composition of wound dressings based on lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate (PMB).
33501867	4	12	theme	membrane	632:639	arg1	formations					641:650	CS/PMB membrane formations	625:650	CS/PMB membrane formations	625:650	The influences of different experimental limitations on CS/PMB membrane formations were examined.
33501867	9	13	from	biomaterial	1162:1172	arg1	model					1253:1257	an animal model	1243:1257	an animal model	1243:1257	These findings indicate that LCH-CS/PMB-based bandaging materials could be a potential innovative biomaterial for tissue repair and regeneration for wound healing applications in an animal model.
33501867	8	14	theme	LCH-CS/PMB-treated	1019:1036	arg1	wound					1038:1042	the LCH-CS/PMB-treated wound	1015:1042	the LCH-CS/PMB-treated wound	1015:1042	In contrast to the untreated wounds, rapid healing was perceived in the LCH-CS/PMB-treated wound with less damaging.
33501867	7	15	theme	mouse	920:924	arg1	models					926:931	mouse models	920:931	mouse models	920:931	Moreover, we examined in vivo healing of skin wounds formed in mouse models over 16 days.
33501867	0	16	theme	Local	0:4	arg1	in vitro					101:108	in vitro	101:108	in vitro	101:108	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles delivery for wound healing: in vitro and in vivo tissue regeneration.
33501867	0	16	theme	Local	0:4	arg1	regeneration					129:140	in vivo tissue regeneration	114:140	in vivo tissue regeneration	114:140	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles delivery for wound healing: in vitro and in vivo tissue regeneration.
33501867	0	16	theme	Local	0:4	arg1	nanoparticles					59:71	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles	0:71	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles delivery for wound healing: in vitro and in vivo tissue regeneration.	0:141	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles delivery for wound healing: in vitro and in vivo tissue regeneration.
33501867	1	17	theme	ester-type	187:196	arg1	anesthetics					204:214	ester-type local anesthetics	187:214	ester-type local anesthetics	187:214	In relieving local pains, lidocaine, one of ester-type local anesthetics, has been used.
33501867	0	18	theme	anesthetic	6:15	arg1	in vitro					101:108	in vitro	101:108	in vitro	101:108	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles delivery for wound healing: in vitro and in vivo tissue regeneration.
33501867	0	18	theme	anesthetic	6:15	arg1	regeneration					129:140	in vivo tissue regeneration	114:140	in vivo tissue regeneration	114:140	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles delivery for wound healing: in vitro and in vivo tissue regeneration.
33501867	0	18	theme	anesthetic	6:15	arg1	nanoparticles					59:71	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles	0:71	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles delivery for wound healing: in vitro and in vivo tissue regeneration.	0:141	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles delivery for wound healing: in vitro and in vivo tissue regeneration.
33501867	8	19	theme	less	1049:1052	arg1	damaging					1054:1061	less damaging	1049:1061	less damaging	1049:1061	In contrast to the untreated wounds, rapid healing was perceived in the LCH-CS/PMB-treated wound with less damaging.
33501867	9	20	theme	wound	1213:1217	arg1	healing					1219:1225	wound healing	1213:1225	wound healing	1213:1225	These findings indicate that LCH-CS/PMB-based bandaging materials could be a potential innovative biomaterial for tissue repair and regeneration for wound healing applications in an animal model.
33501867	8	21	dep	wounds	976:981	arg1	contrast					950:957	contrast	950:957	contrast	950:957	In contrast to the untreated wounds, rapid healing was perceived in the LCH-CS/PMB-treated wound with less damaging.
33501867	1	22	theme	local	198:202	arg1	anesthetics					204:214	ester-type local anesthetics	187:214	ester-type local anesthetics	187:214	In relieving local pains, lidocaine, one of ester-type local anesthetics, has been used.
33501867	2	23	theme	chitosan	429:436	arg1	PMB					464:466	PMB	464:466	PMB	464:466	To develop the lidocaine membranes of enhanced local anesthetic effects, we have designed to establish the composition of wound dressings based on lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate (PMB).
33501867	2	23	theme	chitosan	429:436	arg1	sulfate					455:461	lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate	379:461	lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate (PMB)	379:467	To develop the lidocaine membranes of enhanced local anesthetic effects, we have designed to establish the composition of wound dressings based on lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate (PMB).
33501867	2	23	theme	chitosan	429:436	arg1	CS					439:440	CS	439:440	CS	439:440	To develop the lidocaine membranes of enhanced local anesthetic effects, we have designed to establish the composition of wound dressings based on lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate (PMB).
33501867	3	24	theme	LCH	523:525	arg1	solutions					533:541	the LCH oxide solutions	519:541	the LCH oxide solutions within the CS/PMB matrix	519:566	The LCH membranes (LCH-CS/PMB) was fabricated by the LCH oxide solutions within the CS/PMB matrix.
33501867	9	25	theme	bandaging	1110:1118	arg1	materials					1120:1128	LCH-CS/PMB-based bandaging materials	1093:1128	LCH-CS/PMB-based bandaging materials	1093:1128	These findings indicate that LCH-CS/PMB-based bandaging materials could be a potential innovative biomaterial for tissue repair and regeneration for wound healing applications in an animal model.
33501867	9	25	theme	bandaging	1110:1118	arg1	biomaterial					1162:1172	a potential innovative biomaterial	1139:1172	a potential innovative biomaterial for tissue repair and regeneration for wound healing applications	1139:1238	These findings indicate that LCH-CS/PMB-based bandaging materials could be a potential innovative biomaterial for tissue repair and regeneration for wound healing applications in an animal model.
33501867	2	26	theme	lidocaine	379:387	arg1	LCH					399:401	LCH	399:401	LCH	399:401	To develop the lidocaine membranes of enhanced local anesthetic effects, we have designed to establish the composition of wound dressings based on lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate (PMB).
33501867	2	26	theme	lidocaine	379:387	arg1	chloride					389:396	lidocaine chloride	379:396	lidocaine chloride (LCH)	379:402	To develop the lidocaine membranes of enhanced local anesthetic effects, we have designed to establish the composition of wound dressings based on lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate (PMB).
33501867	0	27	theme	polymyxin-chitosan	40:57	arg1	in vitro					101:108	in vitro	101:108	in vitro	101:108	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles delivery for wound healing: in vitro and in vivo tissue regeneration.
33501867	0	27	theme	polymyxin-chitosan	40:57	arg1	regeneration					129:140	in vivo tissue regeneration	114:140	in vivo tissue regeneration	114:140	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles delivery for wound healing: in vitro and in vivo tissue regeneration.
33501867	0	27	theme	polymyxin-chitosan	40:57	arg1	nanoparticles					59:71	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles	0:71	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles delivery for wound healing: in vitro and in vivo tissue regeneration.	0:141	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles delivery for wound healing: in vitro and in vivo tissue regeneration.
33501867	8	28	theme	rapid	984:988	arg1	healing					990:996	rapid healing	984:996	rapid healing	984:996	In contrast to the untreated wounds, rapid healing was perceived in the LCH-CS/PMB-treated wound with less damaging.
33501867	0	29	theme	lidocaine-encapsulated	17:38	arg1	in vitro					101:108	in vitro	101:108	in vitro	101:108	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles delivery for wound healing: in vitro and in vivo tissue regeneration.
33501867	0	29	theme	lidocaine-encapsulated	17:38	arg1	regeneration					129:140	in vivo tissue regeneration	114:140	in vivo tissue regeneration	114:140	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles delivery for wound healing: in vitro and in vivo tissue regeneration.
33501867	0	29	theme	lidocaine-encapsulated	17:38	arg1	nanoparticles					59:71	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles	0:71	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles delivery for wound healing: in vitro and in vivo tissue regeneration.	0:141	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles delivery for wound healing: in vitro and in vivo tissue regeneration.
33501867	5	30	theme	double	671:676	arg1	sizes					696:700	The double membrane particle sizes	667:700	The double membrane particle sizes	667:700	The double membrane particle sizes were evaluated by scanning electron microscopy (HR-SEM).
33501867	4	31	from	influences	573:582	arg1	formations					641:650	CS/PMB membrane formations	625:650	CS/PMB membrane formations	625:650	The influences of different experimental limitations on CS/PMB membrane formations were examined.
33501867	9	32	theme	LCH-CS/PMB-based	1093:1108	arg1	materials					1120:1128	LCH-CS/PMB-based bandaging materials	1093:1128	LCH-CS/PMB-based bandaging materials	1093:1128	These findings indicate that LCH-CS/PMB-based bandaging materials could be a potential innovative biomaterial for tissue repair and regeneration for wound healing applications in an animal model.
33501867	9	32	theme	LCH-CS/PMB-based	1093:1108	arg1	biomaterial					1162:1172	a potential innovative biomaterial	1139:1172	a potential innovative biomaterial for tissue repair and regeneration for wound healing applications	1139:1238	These findings indicate that LCH-CS/PMB-based bandaging materials could be a potential innovative biomaterial for tissue repair and regeneration for wound healing applications in an animal model.
33501867	2	33	theme	anesthetic	405:414	arg1	drug					416:419	anesthetic drug	405:419	anesthetic drug	405:419	To develop the lidocaine membranes of enhanced local anesthetic effects, we have designed to establish the composition of wound dressings based on lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate (PMB).
33501867	2	34	theme	B	453:453	arg1	PMB					464:466	PMB	464:466	PMB	464:466	To develop the lidocaine membranes of enhanced local anesthetic effects, we have designed to establish the composition of wound dressings based on lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate (PMB).
33501867	2	34	theme	B	453:453	arg1	sulfate					455:461	lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate	379:461	lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate (PMB)	379:467	To develop the lidocaine membranes of enhanced local anesthetic effects, we have designed to establish the composition of wound dressings based on lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate (PMB).
33501867	2	34	theme	B	453:453	arg1	CS					439:440	CS	439:440	CS	439:440	To develop the lidocaine membranes of enhanced local anesthetic effects, we have designed to establish the composition of wound dressings based on lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate (PMB).
33501867	5	35	theme	membrane	678:685	arg1	sizes					696:700	The double membrane particle sizes	667:700	The double membrane particle sizes	667:700	The double membrane particle sizes were evaluated by scanning electron microscopy (HR-SEM).
33501867	1	36	used	used	226:229	arg2	one					180:182	one	180:182	one	180:182	In relieving local pains, lidocaine, one of ester-type local anesthetics, has been used.
33501867	1	36	used	used	226:229	arg2	lidocaine					169:177	lidocaine	169:177	lidocaine	169:177	In relieving local pains, lidocaine, one of ester-type local anesthetics, has been used.
33501867	1	36	used	used	226:229	arg2	anesthetics					204:214	ester-type local anesthetics	187:214	ester-type local anesthetics	187:214	In relieving local pains, lidocaine, one of ester-type local anesthetics, has been used.
33501867	1	37	theme	anesthetics	204:214	arg1	lidocaine					169:177	lidocaine	169:177	lidocaine	169:177	In relieving local pains, lidocaine, one of ester-type local anesthetics, has been used.
33501867	1	37	theme	anesthetics	204:214	arg1	anesthetics					204:214	ester-type local anesthetics	187:214	ester-type local anesthetics	187:214	In relieving local pains, lidocaine, one of ester-type local anesthetics, has been used.
33501867	1	37	theme	anesthetics	204:214	arg1	one					180:182	one	180:182	one	180:182	In relieving local pains, lidocaine, one of ester-type local anesthetics, has been used.
33501867	4	38	theme	experimental	597:608	arg1	limitations					610:620	different experimental limitations	587:620	different experimental limitations	587:620	The influences of different experimental limitations on CS/PMB membrane formations were examined.
33501867	2	39	theme	/polymyxin	442:451	arg1	PMB					464:466	PMB	464:466	PMB	464:466	To develop the lidocaine membranes of enhanced local anesthetic effects, we have designed to establish the composition of wound dressings based on lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate (PMB).
33501867	2	39	theme	/polymyxin	442:451	arg1	sulfate					455:461	lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate	379:461	lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate (PMB)	379:467	To develop the lidocaine membranes of enhanced local anesthetic effects, we have designed to establish the composition of wound dressings based on lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate (PMB).
33501867	2	39	theme	/polymyxin	442:451	arg1	CS					439:440	CS	439:440	CS	439:440	To develop the lidocaine membranes of enhanced local anesthetic effects, we have designed to establish the composition of wound dressings based on lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate (PMB).
33501867	5	40	theme	particle	687:694	arg1	sizes					696:700	The double membrane particle sizes	667:700	The double membrane particle sizes	667:700	The double membrane particle sizes were evaluated by scanning electron microscopy (HR-SEM).
33501867	9	41	theme	animal	1246:1251	arg1	model					1253:1257	an animal model	1243:1257	an animal model	1243:1257	These findings indicate that LCH-CS/PMB-based bandaging materials could be a potential innovative biomaterial for tissue repair and regeneration for wound healing applications in an animal model.
33501867	2	42	theme	-loaded	421:427	arg1	chitosan					429:436	lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan	379:436	lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate (PMB)	379:467	To develop the lidocaine membranes of enhanced local anesthetic effects, we have designed to establish the composition of wound dressings based on lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate (PMB).
33501867	4	43	theme	different	587:595	arg1	limitations					610:620	different experimental limitations	587:620	different experimental limitations	587:620	The influences of different experimental limitations on CS/PMB membrane formations were examined.
33501867	0	44	theme	wound	86:90	arg1	healing					92:98	wound healing	86:98	wound healing	86:98	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles delivery for wound healing: in vitro and in vivo tissue regeneration.
33501867	6	45	theme	negative	832:839	arg1	microorganisms					841:854	gram-positive and negative microorganisms	814:854	gram-positive and negative microorganisms	814:854	Additionally, antibacterial efficacy was developed for gram-positive and negative microorganisms.
33501867	3	46	theme	oxide	527:531	arg1	solutions					533:541	the LCH oxide solutions	519:541	the LCH oxide solutions within the CS/PMB matrix	519:566	The LCH membranes (LCH-CS/PMB) was fabricated by the LCH oxide solutions within the CS/PMB matrix.
33501867	0	47	dep	nanoparticles	59:71	arg1	in vitro					101:108	in vitro	101:108	in vitro	101:108	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles delivery for wound healing: in vitro and in vivo tissue regeneration.
33501867	0	47	dep	nanoparticles	59:71	arg1	delivery					73:80	delivery	73:80	delivery	73:80	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles delivery for wound healing: in vitro and in vivo tissue regeneration.
33501867	0	47	dep	nanoparticles	59:71	arg1	regeneration					129:140	in vivo tissue regeneration	114:140	in vivo tissue regeneration	114:140	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles delivery for wound healing: in vitro and in vivo tissue regeneration.
33501867	0	47	dep	nanoparticles	59:71	arg1	nanoparticles					59:71	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles	0:71	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles delivery for wound healing: in vitro and in vivo tissue regeneration.	0:141	Local anesthetic lidocaine-encapsulated polymyxin-chitosan nanoparticles delivery for wound healing: in vitro and in vivo tissue regeneration.
33501867	4	48	theme	CS/PMB	625:630	arg1	formations					641:650	CS/PMB membrane formations	625:650	CS/PMB membrane formations	625:650	The influences of different experimental limitations on CS/PMB membrane formations were examined.
33501867	7	49	theme	skin	898:901	arg1	wounds					903:908	skin wounds	898:908	skin wounds formed in mouse models over 16 days	898:944	Moreover, we examined in vivo healing of skin wounds formed in mouse models over 16 days.
33501867	6	50	theme	gram-positive	814:826	arg1	microorganisms					841:854	gram-positive and negative microorganisms	814:854	gram-positive and negative microorganisms	814:854	Additionally, antibacterial efficacy was developed for gram-positive and negative microorganisms.
33501867	2	51	theme	enhanced	270:277	arg1	effects					296:302	enhanced local anesthetic effects	270:302	enhanced local anesthetic effects	270:302	To develop the lidocaine membranes of enhanced local anesthetic effects, we have designed to establish the composition of wound dressings based on lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate (PMB).
33501867	9	52	theme	repair	1185:1190	arg1	applications					1227:1238	tissue repair and regeneration for wound healing applications	1178:1238	tissue repair and regeneration for wound healing applications	1178:1238	These findings indicate that LCH-CS/PMB-based bandaging materials could be a potential innovative biomaterial for tissue repair and regeneration for wound healing applications in an animal model.
33501867	1	53	theme	local	156:160	arg1	pains					162:166	local pains	156:166	local pains	156:166	In relieving local pains, lidocaine, one of ester-type local anesthetics, has been used.
33501867	7	54	theme	wounds	903:908	arg1	healing					887:893	in vivo healing	879:893	in vivo healing of skin wounds formed in mouse models over 16 days	879:944	Moreover, we examined in vivo healing of skin wounds formed in mouse models over 16 days.
33501867	2	55	theme	local	279:283	arg1	effects					296:302	enhanced local anesthetic effects	270:302	enhanced local anesthetic effects	270:302	To develop the lidocaine membranes of enhanced local anesthetic effects, we have designed to establish the composition of wound dressings based on lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate (PMB).
33501867	9	56	theme	potential	1141:1149	arg1	materials					1120:1128	LCH-CS/PMB-based bandaging materials	1093:1128	LCH-CS/PMB-based bandaging materials	1093:1128	These findings indicate that LCH-CS/PMB-based bandaging materials could be a potential innovative biomaterial for tissue repair and regeneration for wound healing applications in an animal model.
33501867	9	56	theme	potential	1141:1149	arg1	biomaterial					1162:1172	a potential innovative biomaterial	1139:1172	a potential innovative biomaterial for tissue repair and regeneration for wound healing applications	1139:1238	These findings indicate that LCH-CS/PMB-based bandaging materials could be a potential innovative biomaterial for tissue repair and regeneration for wound healing applications in an animal model.
33501867	5	57	theme	scanning	720:727	arg1	HR-SEM					750:755	HR-SEM	750:755	HR-SEM	750:755	The double membrane particle sizes were evaluated by scanning electron microscopy (HR-SEM).
33501867	5	57	theme	scanning	720:727	arg1	microscopy					738:747	scanning electron microscopy	720:747	scanning electron microscopy (HR-SEM)	720:756	The double membrane particle sizes were evaluated by scanning electron microscopy (HR-SEM).
33501867	3	58	theme	CS/PMB	554:559	arg1	matrix					561:566	the CS/PMB matrix	550:566	the CS/PMB matrix	550:566	The LCH membranes (LCH-CS/PMB) was fabricated by the LCH oxide solutions within the CS/PMB matrix.
33501867	9	59	theme	innovative	1151:1160	arg1	materials					1120:1128	LCH-CS/PMB-based bandaging materials	1093:1128	LCH-CS/PMB-based bandaging materials	1093:1128	These findings indicate that LCH-CS/PMB-based bandaging materials could be a potential innovative biomaterial for tissue repair and regeneration for wound healing applications in an animal model.
33501867	9	59	theme	innovative	1151:1160	arg1	biomaterial					1162:1172	a potential innovative biomaterial	1139:1172	a potential innovative biomaterial for tissue repair and regeneration for wound healing applications	1139:1238	These findings indicate that LCH-CS/PMB-based bandaging materials could be a potential innovative biomaterial for tissue repair and regeneration for wound healing applications in an animal model.
33501867	3	60	theme	LCH	474:476	arg1	LCH-CS/PMB					489:498	LCH-CS/PMB	489:498	LCH-CS/PMB	489:498	The LCH membranes (LCH-CS/PMB) was fabricated by the LCH oxide solutions within the CS/PMB matrix.
33501867	3	60	theme	LCH	474:476	arg1	membranes					478:486	The LCH membranes	470:486	The LCH membranes (LCH-CS/PMB)	470:499	The LCH membranes (LCH-CS/PMB) was fabricated by the LCH oxide solutions within the CS/PMB matrix.
33501867	4	61	theme	limitations	610:620	arg1	influences					573:582	The influences	569:582	The influences of different experimental limitations on CS/PMB membrane formations	569:650	The influences of different experimental limitations on CS/PMB membrane formations were examined.
33501867	5	62	theme	electron	729:736	arg1	HR-SEM					750:755	HR-SEM	750:755	HR-SEM	750:755	The double membrane particle sizes were evaluated by scanning electron microscopy (HR-SEM).
33501867	5	62	theme	electron	729:736	arg1	microscopy					738:747	scanning electron microscopy	720:747	scanning electron microscopy (HR-SEM)	720:756	The double membrane particle sizes were evaluated by scanning electron microscopy (HR-SEM).
33501867	2	63	theme	lidocaine	247:255	arg1	membranes					257:265	the lidocaine membranes	243:265	the lidocaine membranes of enhanced local anesthetic effects	243:302	To develop the lidocaine membranes of enhanced local anesthetic effects, we have designed to establish the composition of wound dressings based on lidocaine chloride (LCH) (anesthetic drug)-loaded chitosan (CS)/polymyxin B sulfate (PMB).
32038603	10	0	from	enriched	1733:1740	arg1	cecum					1749:1753	the cecum	1745:1753	the cecum of low FE pigs	1745:1768	The bacteria from the genus Prevotella was highly enriched in the cecum of low FE pigs, which may impair the establishment of a more effective nutrient harvesting microbiota because of the interaction between Prevotella and other benefical microbes.
32038603	9	1	theme	database	1518:1525	arg1	annotation					1527:1536	the CARD database annotation	1509:1536	the CARD database annotation	1509:1536	Analysis of antibiotic resistance based on the CARD database annotation indicated that the macB resistant gene might play an important role in shaping the microbial community in the cecum of pigs with contrasting FE.
32038603	10	2	theme	FE	1762:1763	arg1	pigs					1765:1768	low FE pigs	1758:1768	low FE pigs	1758:1768	The bacteria from the genus Prevotella was highly enriched in the cecum of low FE pigs, which may impair the establishment of a more effective nutrient harvesting microbiota because of the interaction between Prevotella and other benefical microbes.
32038603	3	3	theme	diverse	511:517	arg1	cecum					542:546	cecum	542:546	cecum	542:546	This study characterized the microbiomes within the intestine of FE contrasting Duroc × (Landrace × Yorkshire) (DLY) pigs with a comprehensive representation of diverse sampling sites (ileum, cecum, and colon) and a metagenomic sequencing approach.
32038603	3	3	theme	diverse	511:517	arg1	colon					553:557	colon	553:557	colon	553:557	This study characterized the microbiomes within the intestine of FE contrasting Duroc × (Landrace × Yorkshire) (DLY) pigs with a comprehensive representation of diverse sampling sites (ileum, cecum, and colon) and a metagenomic sequencing approach.
32038603	3	3	theme	diverse	511:517	arg1	ileum					535:539	ileum	535:539	ileum	535:539	This study characterized the microbiomes within the intestine of FE contrasting Duroc × (Landrace × Yorkshire) (DLY) pigs with a comprehensive representation of diverse sampling sites (ileum, cecum, and colon) and a metagenomic sequencing approach.
32038603	3	3	theme	diverse	511:517	arg1	sites					528:532	diverse sampling sites	511:532	diverse sampling sites (ileum, cecum, and colon)	511:558	This study characterized the microbiomes within the intestine of FE contrasting Duroc × (Landrace × Yorkshire) (DLY) pigs with a comprehensive representation of diverse sampling sites (ileum, cecum, and colon) and a metagenomic sequencing approach.
32038603	11	4	theme	microbial	1982:1990	arg1	compositions					1992:2003	the microbial compositions	1978:2003	the microbial compositions	1978:2003	These findings improved our understanding of the microbial compositions in the different gut locations of DLY pigs and identified many biomarkers associated with FE variation wich may be used to develop strategies to improve FE in pigs.
32038603	2	5	theme	FE-associated	260:272	arg1	taxa					284:287	identified swine FE-associated bacterial taxa	243:287	identified swine FE-associated bacterial taxa	243:287	Previous studies that identified swine FE-associated bacterial taxa were limited in either sampling sites or sequencing methods.
32038603	11	6	theme	different	2012:2020	arg1	locations					2026:2034	the different gut locations	2008:2034	the different gut locations of DLY pigs	2008:2046	These findings improved our understanding of the microbial compositions in the different gut locations of DLY pigs and identified many biomarkers associated with FE variation wich may be used to develop strategies to improve FE in pigs.
32038603	11	7	theme	pigs	2043:2046	arg1	locations					2026:2034	the different gut locations	2008:2034	the different gut locations of DLY pigs	2008:2046	These findings improved our understanding of the microbial compositions in the different gut locations of DLY pigs and identified many biomarkers associated with FE variation wich may be used to develop strategies to improve FE in pigs.
32038603	9	8	theme	resistant	1562:1570	arg1	gene					1572:1575	the macB resistant gene	1553:1575	the macB resistant gene	1553:1575	Analysis of antibiotic resistance based on the CARD database annotation indicated that the macB resistant gene might play an important role in shaping the microbial community in the cecum of pigs with contrasting FE.
32038603	7	9	theme	LEfSe	1289:1293	arg1	analysis					1295:1302	LEfSe analysis	1289:1302	LEfSe analysis	1289:1302	We identified 12 phyla, 17 genera, and 39 species (e.g., Treponema porcinum, Treponema bryantii, and Firmicutes bacterium CAG:110) that were potentially associated with swine FE variation in cecum microbiota through LEfSe analysis.
32038603	6	10	theme	low	1060:1062	arg1	pigs					1067:1070	the low FE pigs	1056:1070	the low FE pigs	1056:1070	We found in cecum that the high FE pigs had slightly higher richness and evenness in their micriobiota than the low FE pigs.
32038603	2	11	theme	sampling	312:319	arg1	sites					321:325	sampling sites	312:325	sampling sites	312:325	Previous studies that identified swine FE-associated bacterial taxa were limited in either sampling sites or sequencing methods.
32038603	1	12	theme	pig	205:207	arg1	production					209:218	pig production	205:218	pig production	205:218	Greater feed efficiency (FE) is critical in increasing profitability while reducing the environmental impact of pig production.
32038603	3	13	theme	sequencing	578:587	arg1	approach					589:596	a metagenomic sequencing approach	564:596	a metagenomic sequencing approach	564:596	This study characterized the microbiomes within the intestine of FE contrasting Duroc × (Landrace × Yorkshire) (DLY) pigs with a comprehensive representation of diverse sampling sites (ileum, cecum, and colon) and a metagenomic sequencing approach.
32038603	7	14	theme	FE	1248:1249	arg1	variation					1251:1259	swine FE variation	1242:1259	swine FE variation in cecum microbiota through LEfSe analysis	1242:1302	We identified 12 phyla, 17 genera, and 39 species (e.g., Treponema porcinum, Treponema bryantii, and Firmicutes bacterium CAG:110) that were potentially associated with swine FE variation in cecum microbiota through LEfSe analysis.
32038603	10	15	theme	nutrient	1826:1833	arg1	establishment					1792:1804	the establishment	1788:1804	the establishment of a more effective nutrient harvesting microbiota because of the interaction between Prevotella and other benefical microbes	1788:1930	The bacteria from the genus Prevotella was highly enriched in the cecum of low FE pigs, which may impair the establishment of a more effective nutrient harvesting microbiota because of the interaction between Prevotella and other benefical microbes.
32038603	2	16	theme	Previous	221:228	arg1	studies					230:236	Previous studies	221:236	Previous studies that identified swine FE-associated bacterial taxa	221:287	Previous studies that identified swine FE-associated bacterial taxa were limited in either sampling sites or sequencing methods.
32038603	5	17	theme	taxonomic	843:851	arg1	composition					853:863	similar microbial taxonomic composition	825:863	similar microbial taxonomic composition	825:863	The results revealed that the cecum and colon had similar microbial taxonomic composition and function, and had higher capacity in polysaccharide metabolism than the ileum.
32038603	8	18	theme	high	1342:1345	arg1	pigs					1350:1353	the high FE pigs	1338:1353	the high FE pigs	1338:1353	Species enriched in the cecum of the high FE pigs had a greater ability to utilize dietary polysaccharides and dietary protein according to the KEGG annotation.
32038603	2	19	theme	bacterial	274:282	arg1	taxa					284:287	identified swine FE-associated bacterial taxa	243:287	identified swine FE-associated bacterial taxa	243:287	Previous studies that identified swine FE-associated bacterial taxa were limited in either sampling sites or sequencing methods.
32038603	7	20	from	variation	1251:1259	arg1	microbiota					1270:1279	cecum microbiota	1264:1279	cecum microbiota	1264:1279	We identified 12 phyla, 17 genera, and 39 species (e.g., Treponema porcinum, Treponema bryantii, and Firmicutes bacterium CAG:110) that were potentially associated with swine FE variation in cecum microbiota through LEfSe analysis.
32038603	9	21	theme	microbial	1621:1629	arg1	community					1631:1639	the microbial community	1617:1639	the microbial community	1617:1639	Analysis of antibiotic resistance based on the CARD database annotation indicated that the macB resistant gene might play an important role in shaping the microbial community in the cecum of pigs with contrasting FE.
32038603	8	22	theme	pigs	1350:1353	arg1	cecum					1329:1333	the cecum	1325:1333	the cecum of the high FE pigs	1325:1353	Species enriched in the cecum of the high FE pigs had a greater ability to utilize dietary polysaccharides and dietary protein according to the KEGG annotation.
32038603	5	23	contain	had	821:823	arg2	function					869:876	function	869:876	function	869:876	The results revealed that the cecum and colon had similar microbial taxonomic composition and function, and had higher capacity in polysaccharide metabolism than the ileum.
32038603	5	23	contain	had	821:823	arg2	composition					853:863	similar microbial taxonomic composition	825:863	similar microbial taxonomic composition	825:863	The results revealed that the cecum and colon had similar microbial taxonomic composition and function, and had higher capacity in polysaccharide metabolism than the ileum.
32038603	5	23	contain	had	821:823	arg1	colon					815:819	colon	815:819	colon	815:819	The results revealed that the cecum and colon had similar microbial taxonomic composition and function, and had higher capacity in polysaccharide metabolism than the ileum.
32038603	5	23	contain	had	821:823	arg1	cecum					805:809	cecum	805:809	cecum	805:809	The results revealed that the cecum and colon had similar microbial taxonomic composition and function, and had higher capacity in polysaccharide metabolism than the ileum.
32038603	0	24	theme	Contrasting	64:74	arg1	Efficiency					81:90	Contrasting Feed Efficiency	64:90	Contrasting Feed Efficiency	64:90	Metagenomic Characterization of Intestinal Regions in Pigs With Contrasting Feed Efficiency.
32038603	2	25	theme	swine	254:258	arg1	taxa					284:287	identified swine FE-associated bacterial taxa	243:287	identified swine FE-associated bacterial taxa	243:287	Previous studies that identified swine FE-associated bacterial taxa were limited in either sampling sites or sequencing methods.
32038603	2	26	theme	identified	243:252	arg1	taxa					284:287	identified swine FE-associated bacterial taxa	243:287	identified swine FE-associated bacterial taxa	243:287	Previous studies that identified swine FE-associated bacterial taxa were limited in either sampling sites or sequencing methods.
32038603	10	27	theme	other	1907:1911	arg1	microbes					1923:1930	other benefical microbes	1907:1930	other benefical microbes	1907:1930	The bacteria from the genus Prevotella was highly enriched in the cecum of low FE pigs, which may impair the establishment of a more effective nutrient harvesting microbiota because of the interaction between Prevotella and other benefical microbes.
32038603	5	28	theme	similar	825:831	arg1	composition					853:863	similar microbial taxonomic composition	825:863	similar microbial taxonomic composition	825:863	The results revealed that the cecum and colon had similar microbial taxonomic composition and function, and had higher capacity in polysaccharide metabolism than the ileum.
32038603	9	29	theme	pigs	1657:1660	arg1	cecum					1648:1652	the cecum	1644:1652	the cecum of pigs with contrasting FE	1644:1680	Analysis of antibiotic resistance based on the CARD database annotation indicated that the macB resistant gene might play an important role in shaping the microbial community in the cecum of pigs with contrasting FE.
32038603	0	30	from	Pigs	54:57	arg1	Characterization					12:27	Metagenomic Characterization	0:27	Metagenomic Characterization of Intestinal Regions in Pigs With Contrasting Feed Efficiency.	0:91	Metagenomic Characterization of Intestinal Regions in Pigs With Contrasting Feed Efficiency.
32038603	11	31	theme	many	2063:2066	arg1	biomarkers					2068:2077	many biomarkers	2063:2077	many biomarkers associated with FE variation wich	2063:2111	These findings improved our understanding of the microbial compositions in the different gut locations of DLY pigs and identified many biomarkers associated with FE variation wich may be used to develop strategies to improve FE in pigs.
32038603	5	32	theme	polysaccharide	906:919	arg1	metabolism					921:930	polysaccharide metabolism	906:930	polysaccharide metabolism	906:930	The results revealed that the cecum and colon had similar microbial taxonomic composition and function, and had higher capacity in polysaccharide metabolism than the ileum.
32038603	9	33	theme	contrasting	1667:1677	arg1	FE					1679:1680	contrasting FE	1667:1680	contrasting FE	1667:1680	Analysis of antibiotic resistance based on the CARD database annotation indicated that the macB resistant gene might play an important role in shaping the microbial community in the cecum of pigs with contrasting FE.
32038603	0	34	from	Regions	43:49	arg1	Pigs					54:57	Pigs	54:57	Pigs With Contrasting Feed Efficiency	54:90	Metagenomic Characterization of Intestinal Regions in Pigs With Contrasting Feed Efficiency.
32038603	11	35	theme	FE	2095:2096	arg1	wich					2108:2111	FE variation wich	2095:2111	FE variation wich	2095:2111	These findings improved our understanding of the microbial compositions in the different gut locations of DLY pigs and identified many biomarkers associated with FE variation wich may be used to develop strategies to improve FE in pigs.
32038603	3	36	theme	Landrace	439:446	arg1	Yorkshire					450:458	Landrace × Yorkshire	439:458	Landrace × Yorkshire	439:458	This study characterized the microbiomes within the intestine of FE contrasting Duroc × (Landrace × Yorkshire) (DLY) pigs with a comprehensive representation of diverse sampling sites (ileum, cecum, and colon) and a metagenomic sequencing approach.
32038603	3	36	theme	Landrace	439:446	arg1	×					436:436	Duroc ×	430:436	Duroc × (Landrace × Yorkshire) (DLY) pigs	430:470	This study characterized the microbiomes within the intestine of FE contrasting Duroc × (Landrace × Yorkshire) (DLY) pigs with a comprehensive representation of diverse sampling sites (ileum, cecum, and colon) and a metagenomic sequencing approach.
32038603	0	37	theme	Metagenomic	0:10	arg1	Characterization					12:27	Metagenomic Characterization	0:27	Metagenomic Characterization of Intestinal Regions in Pigs With Contrasting Feed Efficiency.	0:91	Metagenomic Characterization of Intestinal Regions in Pigs With Contrasting Feed Efficiency.
32038603	1	38	theme	feed	101:104	arg1	FE					118:119	FE	118:119	FE	118:119	Greater feed efficiency (FE) is critical in increasing profitability while reducing the environmental impact of pig production.
32038603	1	38	theme	feed	101:104	arg1	efficiency					106:115	Greater feed efficiency	93:115	Greater feed efficiency (FE)	93:120	Greater feed efficiency (FE) is critical in increasing profitability while reducing the environmental impact of pig production.
32038603	6	39	theme	FE	980:981	arg1	pigs					983:986	the high FE pigs	971:986	the high FE pigs	971:986	We found in cecum that the high FE pigs had slightly higher richness and evenness in their micriobiota than the low FE pigs.
32038603	8	40	theme	dietary	1416:1422	arg1	protein					1424:1430	dietary protein	1416:1430	dietary protein	1416:1430	Species enriched in the cecum of the high FE pigs had a greater ability to utilize dietary polysaccharides and dietary protein according to the KEGG annotation.
32038603	4	41	theme	extreme	698:704	arg1	phenotypes					706:715	extreme phenotypes	698:715	extreme phenotypes	698:715	A total of 226 pigs were ranked according to their FE between weaning to 140 day old, and six with extreme phenotypes were selected, three for each of the high and low groups.
32038603	3	42	dep	sites	528:532	arg1	cecum					542:546	cecum	542:546	cecum	542:546	This study characterized the microbiomes within the intestine of FE contrasting Duroc × (Landrace × Yorkshire) (DLY) pigs with a comprehensive representation of diverse sampling sites (ileum, cecum, and colon) and a metagenomic sequencing approach.
32038603	3	42	dep	sites	528:532	arg1	colon					553:557	colon	553:557	colon	553:557	This study characterized the microbiomes within the intestine of FE contrasting Duroc × (Landrace × Yorkshire) (DLY) pigs with a comprehensive representation of diverse sampling sites (ileum, cecum, and colon) and a metagenomic sequencing approach.
32038603	3	42	dep	sites	528:532	arg1	ileum					535:539	ileum	535:539	ileum	535:539	This study characterized the microbiomes within the intestine of FE contrasting Duroc × (Landrace × Yorkshire) (DLY) pigs with a comprehensive representation of diverse sampling sites (ileum, cecum, and colon) and a metagenomic sequencing approach.
32038603	3	42	dep	sites	528:532	arg1	sites					528:532	diverse sampling sites	511:532	diverse sampling sites (ileum, cecum, and colon)	511:558	This study characterized the microbiomes within the intestine of FE contrasting Duroc × (Landrace × Yorkshire) (DLY) pigs with a comprehensive representation of diverse sampling sites (ileum, cecum, and colon) and a metagenomic sequencing approach.
32038603	7	43	theme	bacterium	1185:1193	arg1	CAG:110					1195:1201	Firmicutes bacterium CAG:110	1174:1201	Firmicutes bacterium CAG:110	1174:1201	We identified 12 phyla, 17 genera, and 39 species (e.g., Treponema porcinum, Treponema bryantii, and Firmicutes bacterium CAG:110) that were potentially associated with swine FE variation in cecum microbiota through LEfSe analysis.
32038603	3	44	theme	×	436:436	arg1	pigs					467:470	Duroc × (Landrace × Yorkshire) (DLY) pigs	430:470	Duroc × (Landrace × Yorkshire) (DLY) pigs	430:470	This study characterized the microbiomes within the intestine of FE contrasting Duroc × (Landrace × Yorkshire) (DLY) pigs with a comprehensive representation of diverse sampling sites (ileum, cecum, and colon) and a metagenomic sequencing approach.
32038603	4	45	theme	high	754:757	arg1	groups					767:772	the high and low groups	750:772	the high and low groups	750:772	A total of 226 pigs were ranked according to their FE between weaning to 140 day old, and six with extreme phenotypes were selected, three for each of the high and low groups.
32038603	0	46	theme	Regions	43:49	arg1	Characterization					12:27	Metagenomic Characterization	0:27	Metagenomic Characterization of Intestinal Regions in Pigs With Contrasting Feed Efficiency.	0:91	Metagenomic Characterization of Intestinal Regions in Pigs With Contrasting Feed Efficiency.
32038603	4	47	theme	low	763:765	arg1	groups					767:772	the high and low groups	750:772	the high and low groups	750:772	A total of 226 pigs were ranked according to their FE between weaning to 140 day old, and six with extreme phenotypes were selected, three for each of the high and low groups.
32038603	3	48	theme	comprehensive	479:491	arg1	representation					493:506	a comprehensive representation	477:506	a comprehensive representation of diverse sampling sites (ileum, cecum, and colon) and a metagenomic sequencing approach	477:596	This study characterized the microbiomes within the intestine of FE contrasting Duroc × (Landrace × Yorkshire) (DLY) pigs with a comprehensive representation of diverse sampling sites (ileum, cecum, and colon) and a metagenomic sequencing approach.
32038603	9	49	theme	resistance	1489:1498	arg1	Analysis					1466:1473	Analysis	1466:1473	Analysis of antibiotic resistance based on the CARD database annotation	1466:1536	Analysis of antibiotic resistance based on the CARD database annotation indicated that the macB resistant gene might play an important role in shaping the microbial community in the cecum of pigs with contrasting FE.
32038603	0	50	with	Pigs	54:57	arg1	Efficiency					81:90	Contrasting Feed Efficiency	64:90	Contrasting Feed Efficiency	64:90	Metagenomic Characterization of Intestinal Regions in Pigs With Contrasting Feed Efficiency.
32038603	3	51	theme	sampling	519:526	arg1	cecum					542:546	cecum	542:546	cecum	542:546	This study characterized the microbiomes within the intestine of FE contrasting Duroc × (Landrace × Yorkshire) (DLY) pigs with a comprehensive representation of diverse sampling sites (ileum, cecum, and colon) and a metagenomic sequencing approach.
32038603	3	51	theme	sampling	519:526	arg1	colon					553:557	colon	553:557	colon	553:557	This study characterized the microbiomes within the intestine of FE contrasting Duroc × (Landrace × Yorkshire) (DLY) pigs with a comprehensive representation of diverse sampling sites (ileum, cecum, and colon) and a metagenomic sequencing approach.
32038603	3	51	theme	sampling	519:526	arg1	ileum					535:539	ileum	535:539	ileum	535:539	This study characterized the microbiomes within the intestine of FE contrasting Duroc × (Landrace × Yorkshire) (DLY) pigs with a comprehensive representation of diverse sampling sites (ileum, cecum, and colon) and a metagenomic sequencing approach.
32038603	3	51	theme	sampling	519:526	arg1	sites					528:532	diverse sampling sites	511:532	diverse sampling sites (ileum, cecum, and colon)	511:558	This study characterized the microbiomes within the intestine of FE contrasting Duroc × (Landrace × Yorkshire) (DLY) pigs with a comprehensive representation of diverse sampling sites (ileum, cecum, and colon) and a metagenomic sequencing approach.
32038603	10	52	from	Prevotella	1711:1720	arg1	enriched					1733:1740	enriched	1733:1740	enriched	1733:1740	The bacteria from the genus Prevotella was highly enriched in the cecum of low FE pigs, which may impair the establishment of a more effective nutrient harvesting microbiota because of the interaction between Prevotella and other benefical microbes.
32038603	10	52	from	Prevotella	1711:1720	arg1	bacteria					1687:1694	The bacteria	1683:1694	The bacteria from the genus Prevotella	1683:1720	The bacteria from the genus Prevotella was highly enriched in the cecum of low FE pigs, which may impair the establishment of a more effective nutrient harvesting microbiota because of the interaction between Prevotella and other benefical microbes.
32038603	9	53	theme	CARD	1513:1516	arg1	annotation					1527:1536	the CARD database annotation	1509:1536	the CARD database annotation	1509:1536	Analysis of antibiotic resistance based on the CARD database annotation indicated that the macB resistant gene might play an important role in shaping the microbial community in the cecum of pigs with contrasting FE.
32038603	10	54	theme	pigs	1765:1768	arg1	cecum					1749:1753	the cecum	1745:1753	the cecum of low FE pigs	1745:1768	The bacteria from the genus Prevotella was highly enriched in the cecum of low FE pigs, which may impair the establishment of a more effective nutrient harvesting microbiota because of the interaction between Prevotella and other benefical microbes.
32038603	11	55	theme	compositions	1992:2003	arg1	understanding					1961:1973	our understanding	1957:1973	our understanding of the microbial compositions	1957:2003	These findings improved our understanding of the microbial compositions in the different gut locations of DLY pigs and identified many biomarkers associated with FE variation wich may be used to develop strategies to improve FE in pigs.
32038603	6	56	theme	higher	1001:1006	arg1	richness					1008:1015	slightly higher richness	992:1015	slightly higher richness	992:1015	We found in cecum that the high FE pigs had slightly higher richness and evenness in their micriobiota than the low FE pigs.
32038603	11	57	dep	findings	1939:1946	arg1	improved					1948:1955	improved	1948:1955	improved our understanding of the microbial compositions in the different gut locations of DLY pigs	1948:2046	These findings improved our understanding of the microbial compositions in the different gut locations of DLY pigs and identified many biomarkers associated with FE variation wich may be used to develop strategies to improve FE in pigs.
32038603	11	57	dep	findings	1939:1946	arg1	identified					2052:2061	identified	2052:2061	identified many biomarkers associated with FE variation wich	2052:2111	These findings improved our understanding of the microbial compositions in the different gut locations of DLY pigs and identified many biomarkers associated with FE variation wich may be used to develop strategies to improve FE in pigs.
32038603	10	58	theme	low	1758:1760	arg1	pigs					1765:1768	low FE pigs	1758:1768	low FE pigs	1758:1768	The bacteria from the genus Prevotella was highly enriched in the cecum of low FE pigs, which may impair the establishment of a more effective nutrient harvesting microbiota because of the interaction between Prevotella and other benefical microbes.
32038603	5	59	from	capacity	894:901	arg1	metabolism					921:930	polysaccharide metabolism	906:930	polysaccharide metabolism	906:930	The results revealed that the cecum and colon had similar microbial taxonomic composition and function, and had higher capacity in polysaccharide metabolism than the ileum.
32038603	11	60	theme	DLY	2039:2041	arg1	pigs					2043:2046	DLY pigs	2039:2046	DLY pigs	2039:2046	These findings improved our understanding of the microbial compositions in the different gut locations of DLY pigs and identified many biomarkers associated with FE variation wich may be used to develop strategies to improve FE in pigs.
32038603	10	61	from	cecum	1749:1753	arg1	enriched					1733:1740	enriched	1733:1740	enriched	1733:1740	The bacteria from the genus Prevotella was highly enriched in the cecum of low FE pigs, which may impair the establishment of a more effective nutrient harvesting microbiota because of the interaction between Prevotella and other benefical microbes.
32038603	10	61	from	cecum	1749:1753	arg1	bacteria					1687:1694	The bacteria	1683:1694	The bacteria from the genus Prevotella	1683:1720	The bacteria from the genus Prevotella was highly enriched in the cecum of low FE pigs, which may impair the establishment of a more effective nutrient harvesting microbiota because of the interaction between Prevotella and other benefical microbes.
32038603	9	62	theme	macB	1557:1560	arg1	gene					1572:1575	the macB resistant gene	1553:1575	the macB resistant gene	1553:1575	Analysis of antibiotic resistance based on the CARD database annotation indicated that the macB resistant gene might play an important role in shaping the microbial community in the cecum of pigs with contrasting FE.
32038603	2	63	theme	sequencing	330:339	arg1	methods					341:347	sequencing methods	330:347	sequencing methods	330:347	Previous studies that identified swine FE-associated bacterial taxa were limited in either sampling sites or sequencing methods.
32038603	1	64	theme	environmental	181:193	arg1	impact					195:200	the environmental impact	177:200	the environmental impact of pig production	177:218	Greater feed efficiency (FE) is critical in increasing profitability while reducing the environmental impact of pig production.
32038603	6	65	contain	had	988:990	arg1	pigs					983:986	the high FE pigs	971:986	the high FE pigs	971:986	We found in cecum that the high FE pigs had slightly higher richness and evenness in their micriobiota than the low FE pigs.
32038603	6	65	contain	had	988:990	arg2	evenness					1021:1028	evenness	1021:1028	evenness	1021:1028	We found in cecum that the high FE pigs had slightly higher richness and evenness in their micriobiota than the low FE pigs.
32038603	6	65	contain	had	988:990	arg2	richness					1008:1015	slightly higher richness	992:1015	slightly higher richness	992:1015	We found in cecum that the high FE pigs had slightly higher richness and evenness in their micriobiota than the low FE pigs.
32038603	6	66	theme	FE	1064:1065	arg1	pigs					1067:1070	the low FE pigs	1056:1070	the low FE pigs	1056:1070	We found in cecum that the high FE pigs had slightly higher richness and evenness in their micriobiota than the low FE pigs.
32038603	5	67	contain	had	883:885	arg2	capacity					894:901	higher capacity	887:901	higher capacity in polysaccharide metabolism	887:930	The results revealed that the cecum and colon had similar microbial taxonomic composition and function, and had higher capacity in polysaccharide metabolism than the ileum.
32038603	5	67	contain	had	883:885	arg1	cecum					805:809	cecum	805:809	cecum	805:809	The results revealed that the cecum and colon had similar microbial taxonomic composition and function, and had higher capacity in polysaccharide metabolism than the ileum.
32038603	9	68	with	pigs	1657:1660	arg1	FE					1679:1680	contrasting FE	1667:1680	contrasting FE	1667:1680	Analysis of antibiotic resistance based on the CARD database annotation indicated that the macB resistant gene might play an important role in shaping the microbial community in the cecum of pigs with contrasting FE.
32038603	1	69	theme	production	209:218	arg1	impact					195:200	the environmental impact	177:200	the environmental impact of pig production	177:218	Greater feed efficiency (FE) is critical in increasing profitability while reducing the environmental impact of pig production.
32038603	9	70	theme	important	1591:1599	arg1	role					1601:1604	an important role	1588:1604	an important role	1588:1604	Analysis of antibiotic resistance based on the CARD database annotation indicated that the macB resistant gene might play an important role in shaping the microbial community in the cecum of pigs with contrasting FE.
32038603	7	71	theme	cecum	1264:1268	arg1	microbiota					1270:1279	cecum microbiota	1264:1279	cecum microbiota	1264:1279	We identified 12 phyla, 17 genera, and 39 species (e.g., Treponema porcinum, Treponema bryantii, and Firmicutes bacterium CAG:110) that were potentially associated with swine FE variation in cecum microbiota through LEfSe analysis.
32038603	4	72	theme	pigs	614:617	arg1	total					601:605	A total	599:605	A total of 226 pigs	599:617	A total of 226 pigs were ranked according to their FE between weaning to 140 day old, and six with extreme phenotypes were selected, three for each of the high and low groups.
32038603	5	73	theme	microbial	833:841	arg1	composition					853:863	similar microbial taxonomic composition	825:863	similar microbial taxonomic composition	825:863	The results revealed that the cecum and colon had similar microbial taxonomic composition and function, and had higher capacity in polysaccharide metabolism than the ileum.
32038603	8	74	theme	FE	1347:1348	arg1	pigs					1350:1353	the high FE pigs	1338:1353	the high FE pigs	1338:1353	Species enriched in the cecum of the high FE pigs had a greater ability to utilize dietary polysaccharides and dietary protein according to the KEGG annotation.
32038603	10	75	theme	effective	1816:1824	arg1	nutrient					1826:1833	a more effective nutrient	1809:1833	a more effective nutrient harvesting microbiota because of the interaction between Prevotella and other benefical microbes	1809:1930	The bacteria from the genus Prevotella was highly enriched in the cecum of low FE pigs, which may impair the establishment of a more effective nutrient harvesting microbiota because of the interaction between Prevotella and other benefical microbes.
32038603	10	76	theme	genus	1705:1709	arg1	Prevotella					1711:1720	the genus Prevotella	1701:1720	the genus Prevotella	1701:1720	The bacteria from the genus Prevotella was highly enriched in the cecum of low FE pigs, which may impair the establishment of a more effective nutrient harvesting microbiota because of the interaction between Prevotella and other benefical microbes.
32038603	9	77	from	shaping	1609:1615	arg1	cecum					1648:1652	the cecum	1644:1652	the cecum of pigs with contrasting FE	1644:1680	Analysis of antibiotic resistance based on the CARD database annotation indicated that the macB resistant gene might play an important role in shaping the microbial community in the cecum of pigs with contrasting FE.
32038603	11	78	used	used	2120:2123	arg2	findings					1939:1946	These findings	1933:1946	These findings improved our understanding of the microbial compositions in the different gut locations of DLY pigs and identified many biomarkers associated with FE variation wich	1933:2111	These findings improved our understanding of the microbial compositions in the different gut locations of DLY pigs and identified many biomarkers associated with FE variation wich may be used to develop strategies to improve FE in pigs.
32038603	7	79	theme	swine	1242:1246	arg1	variation					1251:1259	swine FE variation	1242:1259	swine FE variation in cecum microbiota through LEfSe analysis	1242:1302	We identified 12 phyla, 17 genera, and 39 species (e.g., Treponema porcinum, Treponema bryantii, and Firmicutes bacterium CAG:110) that were potentially associated with swine FE variation in cecum microbiota through LEfSe analysis.
32038603	0	80	theme	Feed	76:79	arg1	Efficiency					81:90	Contrasting Feed Efficiency	64:90	Contrasting Feed Efficiency	64:90	Metagenomic Characterization of Intestinal Regions in Pigs With Contrasting Feed Efficiency.
32038603	3	81	theme	approach	589:596	arg1	representation					493:506	a comprehensive representation	477:506	a comprehensive representation of diverse sampling sites (ileum, cecum, and colon) and a metagenomic sequencing approach	477:596	This study characterized the microbiomes within the intestine of FE contrasting Duroc × (Landrace × Yorkshire) (DLY) pigs with a comprehensive representation of diverse sampling sites (ileum, cecum, and colon) and a metagenomic sequencing approach.
32038603	11	82	theme	variation	2098:2106	arg1	wich					2108:2111	FE variation wich	2095:2111	FE variation wich	2095:2111	These findings improved our understanding of the microbial compositions in the different gut locations of DLY pigs and identified many biomarkers associated with FE variation wich may be used to develop strategies to improve FE in pigs.
32038603	8	83	theme	greater	1361:1367	arg1	ability					1369:1375	a greater ability	1359:1375	a greater ability to utilize dietary polysaccharides	1359:1410	Species enriched in the cecum of the high FE pigs had a greater ability to utilize dietary polysaccharides and dietary protein according to the KEGG annotation.
32038603	0	84	from	Characterization	12:27	arg1	Pigs					54:57	Pigs	54:57	Pigs With Contrasting Feed Efficiency	54:90	Metagenomic Characterization of Intestinal Regions in Pigs With Contrasting Feed Efficiency.
32038603	5	85	theme	higher	887:892	arg1	capacity					894:901	higher capacity	887:901	higher capacity in polysaccharide metabolism	887:930	The results revealed that the cecum and colon had similar microbial taxonomic composition and function, and had higher capacity in polysaccharide metabolism than the ileum.
32038603	8	86	theme	dietary	1388:1394	arg1	polysaccharides					1396:1410	dietary polysaccharides	1388:1410	dietary polysaccharides	1388:1410	Species enriched in the cecum of the high FE pigs had a greater ability to utilize dietary polysaccharides and dietary protein according to the KEGG annotation.
32038603	11	87	theme	gut	2022:2024	arg1	locations					2026:2034	the different gut locations	2008:2034	the different gut locations of DLY pigs	2008:2046	These findings improved our understanding of the microbial compositions in the different gut locations of DLY pigs and identified many biomarkers associated with FE variation wich may be used to develop strategies to improve FE in pigs.
32038603	1	88	theme	Greater	93:99	arg1	FE					118:119	FE	118:119	FE	118:119	Greater feed efficiency (FE) is critical in increasing profitability while reducing the environmental impact of pig production.
32038603	1	88	theme	Greater	93:99	arg1	efficiency					106:115	Greater feed efficiency	93:115	Greater feed efficiency (FE)	93:120	Greater feed efficiency (FE) is critical in increasing profitability while reducing the environmental impact of pig production.
32038603	3	89	theme	FE	415:416	arg1	microbiomes					379:389	the microbiomes	375:389	the microbiomes within the intestine of FE	375:416	This study characterized the microbiomes within the intestine of FE contrasting Duroc × (Landrace × Yorkshire) (DLY) pigs with a comprehensive representation of diverse sampling sites (ileum, cecum, and colon) and a metagenomic sequencing approach.
32038603	3	90	theme	×	448:448	arg1	Yorkshire					450:458	Landrace × Yorkshire	439:458	Landrace × Yorkshire	439:458	This study characterized the microbiomes within the intestine of FE contrasting Duroc × (Landrace × Yorkshire) (DLY) pigs with a comprehensive representation of diverse sampling sites (ileum, cecum, and colon) and a metagenomic sequencing approach.
32038603	3	90	theme	×	448:448	arg1	×					436:436	Duroc ×	430:436	Duroc × (Landrace × Yorkshire) (DLY) pigs	430:470	This study characterized the microbiomes within the intestine of FE contrasting Duroc × (Landrace × Yorkshire) (DLY) pigs with a comprehensive representation of diverse sampling sites (ileum, cecum, and colon) and a metagenomic sequencing approach.
32038603	0	91	theme	Intestinal	32:41	arg1	Regions					43:49	Intestinal Regions	32:49	Intestinal Regions in Pigs With Contrasting Feed Efficiency	32:90	Metagenomic Characterization of Intestinal Regions in Pigs With Contrasting Feed Efficiency.
32038603	3	92	theme	metagenomic	566:576	arg1	approach					589:596	a metagenomic sequencing approach	564:596	a metagenomic sequencing approach	564:596	This study characterized the microbiomes within the intestine of FE contrasting Duroc × (Landrace × Yorkshire) (DLY) pigs with a comprehensive representation of diverse sampling sites (ileum, cecum, and colon) and a metagenomic sequencing approach.
32038603	6	93	theme	high	975:978	arg1	pigs					983:986	the high FE pigs	971:986	the high FE pigs	971:986	We found in cecum that the high FE pigs had slightly higher richness and evenness in their micriobiota than the low FE pigs.
32038603	10	94	theme	benefical	1913:1921	arg1	microbes					1923:1930	other benefical microbes	1907:1930	other benefical microbes	1907:1930	The bacteria from the genus Prevotella was highly enriched in the cecum of low FE pigs, which may impair the establishment of a more effective nutrient harvesting microbiota because of the interaction between Prevotella and other benefical microbes.
32038603	8	95	theme	KEGG	1449:1452	arg1	annotation					1454:1463	the KEGG annotation	1445:1463	the KEGG annotation	1445:1463	Species enriched in the cecum of the high FE pigs had a greater ability to utilize dietary polysaccharides and dietary protein according to the KEGG annotation.
32038603	7	96	dep	species	1115:1121	arg1	e.g.					1124:1127	e.g.	1124:1127	e.g.	1124:1127	We identified 12 phyla, 17 genera, and 39 species (e.g., Treponema porcinum, Treponema bryantii, and Firmicutes bacterium CAG:110) that were potentially associated with swine FE variation in cecum microbiota through LEfSe analysis.
32038603	7	96	dep	species	1115:1121	arg1	CAG:110					1195:1201	Firmicutes bacterium CAG:110	1174:1201	Firmicutes bacterium CAG:110	1174:1201	We identified 12 phyla, 17 genera, and 39 species (e.g., Treponema porcinum, Treponema bryantii, and Firmicutes bacterium CAG:110) that were potentially associated with swine FE variation in cecum microbiota through LEfSe analysis.
32038603	7	96	dep	species	1115:1121	arg1	porcinum					1140:1147	Treponema porcinum	1130:1147	Treponema porcinum	1130:1147	We identified 12 phyla, 17 genera, and 39 species (e.g., Treponema porcinum, Treponema bryantii, and Firmicutes bacterium CAG:110) that were potentially associated with swine FE variation in cecum microbiota through LEfSe analysis.
32038603	7	96	dep	species	1115:1121	arg1	bryantii					1160:1167	Treponema bryantii	1150:1167	Treponema bryantii	1150:1167	We identified 12 phyla, 17 genera, and 39 species (e.g., Treponema porcinum, Treponema bryantii, and Firmicutes bacterium CAG:110) that were potentially associated with swine FE variation in cecum microbiota through LEfSe analysis.
32038603	9	97	theme	antibiotic	1478:1487	arg1	resistance					1489:1498	antibiotic resistance	1478:1498	antibiotic resistance based on the CARD database annotation	1478:1536	Analysis of antibiotic resistance based on the CARD database annotation indicated that the macB resistant gene might play an important role in shaping the microbial community in the cecum of pigs with contrasting FE.
32038603	3	98	theme	Duroc	430:434	arg1	Yorkshire					450:458	Landrace × Yorkshire	439:458	Landrace × Yorkshire	439:458	This study characterized the microbiomes within the intestine of FE contrasting Duroc × (Landrace × Yorkshire) (DLY) pigs with a comprehensive representation of diverse sampling sites (ileum, cecum, and colon) and a metagenomic sequencing approach.
32038603	3	98	theme	Duroc	430:434	arg1	DLY					462:464	DLY	462:464	DLY	462:464	This study characterized the microbiomes within the intestine of FE contrasting Duroc × (Landrace × Yorkshire) (DLY) pigs with a comprehensive representation of diverse sampling sites (ileum, cecum, and colon) and a metagenomic sequencing approach.
32038603	3	98	theme	Duroc	430:434	arg1	×					436:436	Duroc ×	430:436	Duroc × (Landrace × Yorkshire) (DLY) pigs	430:470	This study characterized the microbiomes within the intestine of FE contrasting Duroc × (Landrace × Yorkshire) (DLY) pigs with a comprehensive representation of diverse sampling sites (ileum, cecum, and colon) and a metagenomic sequencing approach.
32038603	11	99	from	FE	2158:2159	arg1	pigs					2164:2167	pigs	2164:2167	pigs	2164:2167	These findings improved our understanding of the microbial compositions in the different gut locations of DLY pigs and identified many biomarkers associated with FE variation wich may be used to develop strategies to improve FE in pigs.
32038603	8	100	contain	had	1355:1357	arg1	Species					1305:1311	Species	1305:1311	Species enriched in the cecum of the high FE pigs	1305:1353	Species enriched in the cecum of the high FE pigs had a greater ability to utilize dietary polysaccharides and dietary protein according to the KEGG annotation.
32038603	8	100	contain	had	1355:1357	arg2	protein					1424:1430	dietary protein	1416:1430	dietary protein	1416:1430	Species enriched in the cecum of the high FE pigs had a greater ability to utilize dietary polysaccharides and dietary protein according to the KEGG annotation.
32038603	8	100	contain	had	1355:1357	arg2	ability					1369:1375	a greater ability	1359:1375	a greater ability to utilize dietary polysaccharides	1359:1410	Species enriched in the cecum of the high FE pigs had a greater ability to utilize dietary polysaccharides and dietary protein according to the KEGG annotation.
32038603	6	101	from	richness	1008:1015	arg1	micriobiota					1039:1049	their micriobiota	1033:1049	their micriobiota	1033:1049	We found in cecum that the high FE pigs had slightly higher richness and evenness in their micriobiota than the low FE pigs.
32038603	7	102	theme	Firmicutes	1174:1183	arg1	CAG:110					1195:1201	Firmicutes bacterium CAG:110	1174:1201	Firmicutes bacterium CAG:110	1174:1201	We identified 12 phyla, 17 genera, and 39 species (e.g., Treponema porcinum, Treponema bryantii, and Firmicutes bacterium CAG:110) that were potentially associated with swine FE variation in cecum microbiota through LEfSe analysis.
32038603	6	103	from	evenness	1021:1028	arg1	micriobiota					1039:1049	their micriobiota	1033:1049	their micriobiota	1033:1049	We found in cecum that the high FE pigs had slightly higher richness and evenness in their micriobiota than the low FE pigs.
32038603	3	104	theme	sites	528:532	arg1	representation					493:506	a comprehensive representation	477:506	a comprehensive representation of diverse sampling sites (ileum, cecum, and colon) and a metagenomic sequencing approach	477:596	This study characterized the microbiomes within the intestine of FE contrasting Duroc × (Landrace × Yorkshire) (DLY) pigs with a comprehensive representation of diverse sampling sites (ileum, cecum, and colon) and a metagenomic sequencing approach.
34641376	1	0	used	used	277:280	arg2	parts					178:182	Different parts	168:182	Different parts of Araucaria bidiwillii (bunya pin) trees, such as nuts, seeds, bark, and shoots,	168:264	Different parts of Araucaria bidiwillii (bunya pin) trees, such as nuts, seeds, bark, and shoots, are widely used in cooking, tea, and traditional medicines around the world.
34641376	7	1	theme	reference	1190:1198	arg1	groups					1206:1211	the control and reference drugs groups	1174:1211	groups	1206:1211	The ABSEO and its nanoemulsion exhibited significant inflammation suppression in carrageenan-induced rat paw edema model, in both oral (50 and 100 mg/kg) and topical (5% in soyabean oil) routes, compared to the control and reference drugs groups.
34641376	10	2	theme	numerous	1794:1801	arg1	ailments					1833:1840	numerous inflammatory and hyperthermia ailments	1794:1840	numerous inflammatory and hyperthermia ailments that could be attributed to its active constituents	1794:1892	These findings advised that the use of ABSEO and its nanoemulsion against numerous inflammatory and hyperthermia ailments that could be attributed to its active constituents.
34641376	8	3	theme	histopathological	1393:1409	arg1	studies					1411:1417	the histopathological studies	1389:1417	the histopathological studies	1389:1417	All the results demonstrated the significant inflammation reduction via the inflammatory cytokines (IL-1β and IL8), nitrosative (NO), and prostaglandin E2 (PGE2) supported by the histopathological studies and immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues.
34641376	1	4	theme	Araucaria	187:195	arg1	shoots					258:263	shoots	258:263	shoots	258:263	Different parts of Araucaria bidiwillii (bunya pin) trees, such as nuts, seeds, bark, and shoots, are widely used in cooking, tea, and traditional medicines around the world.
34641376	1	4	theme	Araucaria	187:195	arg1	seeds					241:245	seeds	241:245	seeds	241:245	Different parts of Araucaria bidiwillii (bunya pin) trees, such as nuts, seeds, bark, and shoots, are widely used in cooking, tea, and traditional medicines around the world.
34641376	1	4	theme	Araucaria	187:195	arg1	bark					248:251	bark	248:251	bark	248:251	Different parts of Araucaria bidiwillii (bunya pin) trees, such as nuts, seeds, bark, and shoots, are widely used in cooking, tea, and traditional medicines around the world.
34641376	1	4	theme	Araucaria	187:195	arg1	nuts					235:238	nuts	235:238	nuts	235:238	Different parts of Araucaria bidiwillii (bunya pin) trees, such as nuts, seeds, bark, and shoots, are widely used in cooking, tea, and traditional medicines around the world.
34641376	1	4	theme	Araucaria	187:195	arg1	trees					220:224	Araucaria bidiwillii (bunya pin) trees	187:224	Araucaria bidiwillii (bunya pin) trees	187:224	Different parts of Araucaria bidiwillii (bunya pin) trees, such as nuts, seeds, bark, and shoots, are widely used in cooking, tea, and traditional medicines around the world.
34641376	8	5	from	MMP-9	1457:1461	arg1	tissues					1487:1493	paw tissues	1483:1493	paw tissues	1483:1493	All the results demonstrated the significant inflammation reduction via the inflammatory cytokines (IL-1β and IL8), nitrosative (NO), and prostaglandin E2 (PGE2) supported by the histopathological studies and immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues.
34641376	6	6	theme	ABSEO	833:837	arg1	beyerene					844:851	beyerene	844:851	beyerene (20.81%)	844:860	The main compounds of the ABSEO were beyerene (20.81%), α-pinene (16.21%), D-limonene (14.22%), germacrene D (6.69%), β-humulene (4.14%), and sabinene (4.12%).
34641376	6	6	theme	ABSEO	833:837	arg1	compounds					816:824	The main compounds	807:824	The main compounds of the ABSEO	807:837	The main compounds of the ABSEO were beyerene (20.81%), α-pinene (16.21%), D-limonene (14.22%), germacrene D (6.69%), β-humulene (4.14%), and sabinene (4.12%).
34641376	7	7	theme	topical	1125:1131	arg1	routes					1154:1159	both oral (50 and 100 mg/kg) and topical (5% in soyabean oil) routes	1092:1159	both oral (50 and 100 mg/kg) and topical (5% in soyabean oil) routes	1092:1159	The ABSEO and its nanoemulsion exhibited significant inflammation suppression in carrageenan-induced rat paw edema model, in both oral (50 and 100 mg/kg) and topical (5% in soyabean oil) routes, compared to the control and reference drugs groups.
34641376	8	8	from	tissues	1487:1493	arg1	studies					1411:1417	the histopathological studies	1389:1417	the histopathological studies	1389:1417	All the results demonstrated the significant inflammation reduction via the inflammatory cytokines (IL-1β and IL8), nitrosative (NO), and prostaglandin E2 (PGE2) supported by the histopathological studies and immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues.
34641376	8	8	from	tissues	1487:1493	arg1	assessment					1443:1452	immunohistochemical assessment	1423:1452	immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues	1423:1493	All the results demonstrated the significant inflammation reduction via the inflammatory cytokines (IL-1β and IL8), nitrosative (NO), and prostaglandin E2 (PGE2) supported by the histopathological studies and immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues.
34641376	1	9	theme	pin	215:217	arg1	shoots					258:263	shoots	258:263	shoots	258:263	Different parts of Araucaria bidiwillii (bunya pin) trees, such as nuts, seeds, bark, and shoots, are widely used in cooking, tea, and traditional medicines around the world.
34641376	1	9	theme	pin	215:217	arg1	seeds					241:245	seeds	241:245	seeds	241:245	Different parts of Araucaria bidiwillii (bunya pin) trees, such as nuts, seeds, bark, and shoots, are widely used in cooking, tea, and traditional medicines around the world.
34641376	1	9	theme	pin	215:217	arg1	bark					248:251	bark	248:251	bark	248:251	Different parts of Araucaria bidiwillii (bunya pin) trees, such as nuts, seeds, bark, and shoots, are widely used in cooking, tea, and traditional medicines around the world.
34641376	1	9	theme	pin	215:217	arg1	nuts					235:238	nuts	235:238	nuts	235:238	Different parts of Araucaria bidiwillii (bunya pin) trees, such as nuts, seeds, bark, and shoots, are widely used in cooking, tea, and traditional medicines around the world.
34641376	1	9	theme	pin	215:217	arg1	trees					220:224	Araucaria bidiwillii (bunya pin) trees	187:224	Araucaria bidiwillii (bunya pin) trees	187:224	Different parts of Araucaria bidiwillii (bunya pin) trees, such as nuts, seeds, bark, and shoots, are widely used in cooking, tea, and traditional medicines around the world.
34641376	4	10	theme	anti-inflammatory	537:553	arg1	effect					555:560	the in vivo oral and topical anti-inflammatory effect	508:560	the in vivo oral and topical anti-inflammatory effect against carrageenan-induced models, as well as antipyretic potentiality of ABSEO and its nanoemulsion	508:662	Additionally, the in vivo oral and topical anti-inflammatory effect against carrageenan-induced models, as well as antipyretic potentiality of ABSEO and its nanoemulsion were evaluated.
34641376	8	11	theme	MMP-9	1457:1461	arg1	studies					1411:1417	the histopathological studies	1389:1417	the histopathological studies	1389:1417	All the results demonstrated the significant inflammation reduction via the inflammatory cytokines (IL-1β and IL8), nitrosative (NO), and prostaglandin E2 (PGE2) supported by the histopathological studies and immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues.
34641376	8	11	theme	MMP-9	1457:1461	arg1	assessment					1443:1452	immunohistochemical assessment	1423:1452	immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues	1423:1493	All the results demonstrated the significant inflammation reduction via the inflammatory cytokines (IL-1β and IL8), nitrosative (NO), and prostaglandin E2 (PGE2) supported by the histopathological studies and immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues.
34641376	1	12	theme	trees	220:224	arg1	parts					178:182	Different parts	168:182	Different parts of Araucaria bidiwillii (bunya pin) trees, such as nuts, seeds, bark, and shoots,	168:264	Different parts of Araucaria bidiwillii (bunya pin) trees, such as nuts, seeds, bark, and shoots, are widely used in cooking, tea, and traditional medicines around the world.
34641376	6	13	theme	main	811:814	arg1	beyerene					844:851	beyerene	844:851	beyerene (20.81%)	844:860	The main compounds of the ABSEO were beyerene (20.81%), α-pinene (16.21%), D-limonene (14.22%), germacrene D (6.69%), β-humulene (4.14%), and sabinene (4.12%).
34641376	6	13	theme	main	811:814	arg1	compounds					816:824	The main compounds	807:824	The main compounds of the ABSEO	807:837	The main compounds of the ABSEO were beyerene (20.81%), α-pinene (16.21%), D-limonene (14.22%), germacrene D (6.69%), β-humulene (4.14%), and sabinene (4.12%).
34641376	8	14	theme	NF-κβ	1467:1471	arg1	levels					1473:1478	NF-κβ levels	1467:1478	NF-κβ levels in paw tissues	1467:1493	All the results demonstrated the significant inflammation reduction via the inflammatory cytokines (IL-1β and IL8), nitrosative (NO), and prostaglandin E2 (PGE2) supported by the histopathological studies and immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues.
34641376	4	15	theme	ABSEO	637:641	arg1	nanoemulsion					651:662	its nanoemulsion	647:662	its nanoemulsion	647:662	Additionally, the in vivo oral and topical anti-inflammatory effect against carrageenan-induced models, as well as antipyretic potentiality of ABSEO and its nanoemulsion were evaluated.
34641376	4	15	theme	ABSEO	637:641	arg1	potentiality					621:632	antipyretic potentiality	609:632	antipyretic potentiality of ABSEO	609:641	Additionally, the in vivo oral and topical anti-inflammatory effect against carrageenan-induced models, as well as antipyretic potentiality of ABSEO and its nanoemulsion were evaluated.
34641376	4	15	theme	ABSEO	637:641	arg1	models					590:595	carrageenan-induced models	570:595	carrageenan-induced models	570:595	Additionally, the in vivo oral and topical anti-inflammatory effect against carrageenan-induced models, as well as antipyretic potentiality of ABSEO and its nanoemulsion were evaluated.
34641376	0	16	from	Oil	106:108	arg1	Relation					134:141	Relation	134:141	Relation to Chemical Composition	134:165	Oral and Topical Anti-Inflammatory and Antipyretic Potentialities of Araucaria bidiwillii Shoot Essential Oil and Its Nanoemulsion in Relation to Chemical Composition.
34641376	7	17	theme	oral	1097:1100	arg1	routes					1154:1159	both oral (50 and 100 mg/kg) and topical (5% in soyabean oil) routes	1092:1159	both oral (50 and 100 mg/kg) and topical (5% in soyabean oil) routes	1092:1159	The ABSEO and its nanoemulsion exhibited significant inflammation suppression in carrageenan-induced rat paw edema model, in both oral (50 and 100 mg/kg) and topical (5% in soyabean oil) routes, compared to the control and reference drugs groups.
34641376	0	18	theme	Araucaria	69:77	arg1	Oil					106:108	Araucaria bidiwillii Shoot Essential Oil	69:108	Araucaria bidiwillii Shoot Essential Oil	69:108	Oral and Topical Anti-Inflammatory and Antipyretic Potentialities of Araucaria bidiwillii Shoot Essential Oil and Its Nanoemulsion in Relation to Chemical Composition.
34641376	9	19	theme	intramuscular	1677:1689	arg1	injection					1691:1699	intramuscular injection	1677:1699	intramuscular injection of brewer's yeast	1677:1717	Moreover, the oral administration of ABSEO and its nanoemulsion (50 and 100 mg/kg) exhibited antipyretic activity in rats, demonstrated by the inhibition of hyperthermia induced by intramuscular injection of brewer's yeast.
34641376	0	20	theme	Shoot	90:94	arg1	Oil					106:108	Araucaria bidiwillii Shoot Essential Oil	69:108	Araucaria bidiwillii Shoot Essential Oil	69:108	Oral and Topical Anti-Inflammatory and Antipyretic Potentialities of Araucaria bidiwillii Shoot Essential Oil and Its Nanoemulsion in Relation to Chemical Composition.
34641376	0	21	theme	Oil	106:108	arg1	Potentialities					51:64	Oral and Topical Anti-Inflammatory and Antipyretic Potentialities	0:64	Oral and Topical Anti-Inflammatory and Antipyretic Potentialities of Araucaria bidiwillii Shoot Essential Oil and Its Nanoemulsion in Relation to Chemical Composition.	0:166	Oral and Topical Anti-Inflammatory and Antipyretic Potentialities of Araucaria bidiwillii Shoot Essential Oil and Its Nanoemulsion in Relation to Chemical Composition.
34641376	0	22	from	Potentialities	51:64	arg1	Relation					134:141	Relation	134:141	Relation to Chemical Composition	134:165	Oral and Topical Anti-Inflammatory and Antipyretic Potentialities of Araucaria bidiwillii Shoot Essential Oil and Its Nanoemulsion in Relation to Chemical Composition.
34641376	0	23	theme	Oral	0:3	arg1	Potentialities					51:64	Oral and Topical Anti-Inflammatory and Antipyretic Potentialities	0:64	Oral and Topical Anti-Inflammatory and Antipyretic Potentialities of Araucaria bidiwillii Shoot Essential Oil and Its Nanoemulsion in Relation to Chemical Composition.	0:166	Oral and Topical Anti-Inflammatory and Antipyretic Potentialities of Araucaria bidiwillii Shoot Essential Oil and Its Nanoemulsion in Relation to Chemical Composition.
34641376	8	24	theme	significant	1247:1257	arg1	reduction					1272:1280	the significant inflammation reduction	1243:1280	the significant inflammation reduction via the inflammatory cytokines (IL-1β and IL8)	1243:1327	All the results demonstrated the significant inflammation reduction via the inflammatory cytokines (IL-1β and IL8), nitrosative (NO), and prostaglandin E2 (PGE2) supported by the histopathological studies and immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues.
34641376	0	25	theme	Topical	9:15	arg1	Potentialities					51:64	Oral and Topical Anti-Inflammatory and Antipyretic Potentialities	0:64	Oral and Topical Anti-Inflammatory and Antipyretic Potentialities of Araucaria bidiwillii Shoot Essential Oil and Its Nanoemulsion in Relation to Chemical Composition.	0:166	Oral and Topical Anti-Inflammatory and Antipyretic Potentialities of Araucaria bidiwillii Shoot Essential Oil and Its Nanoemulsion in Relation to Chemical Composition.
34641376	7	26	theme	edema	1076:1080	arg1	model					1082:1086	carrageenan-induced rat paw edema model	1048:1086	carrageenan-induced rat paw edema model	1048:1086	The ABSEO and its nanoemulsion exhibited significant inflammation suppression in carrageenan-induced rat paw edema model, in both oral (50 and 100 mg/kg) and topical (5% in soyabean oil) routes, compared to the control and reference drugs groups.
34641376	9	27	theme	oral	1510:1513	arg1	administration					1515:1528	the oral administration	1506:1528	the oral administration of ABSEO and its nanoemulsion (50 and 100 mg/kg)	1506:1577	Moreover, the oral administration of ABSEO and its nanoemulsion (50 and 100 mg/kg) exhibited antipyretic activity in rats, demonstrated by the inhibition of hyperthermia induced by intramuscular injection of brewer's yeast.
34641376	2	28	theme	essential	354:362	arg1	EO					369:370	EO	369:370	EO	369:370	The shoots essential oil (EO) has not yet been studied.
34641376	2	28	theme	essential	354:362	arg1	oil					364:366	The shoots essential oil	343:366	The shoots essential oil (EO)	343:371	The shoots essential oil (EO) has not yet been studied.
34641376	7	29	theme	rat	1068:1070	arg1	edema					1076:1080	carrageenan-induced rat paw edema	1048:1080	carrageenan-induced rat paw edema model	1048:1086	The ABSEO and its nanoemulsion exhibited significant inflammation suppression in carrageenan-induced rat paw edema model, in both oral (50 and 100 mg/kg) and topical (5% in soyabean oil) routes, compared to the control and reference drugs groups.
34641376	8	30	from	assessment	1443:1452	arg1	tissues					1487:1493	paw tissues	1483:1493	paw tissues	1483:1493	All the results demonstrated the significant inflammation reduction via the inflammatory cytokines (IL-1β and IL8), nitrosative (NO), and prostaglandin E2 (PGE2) supported by the histopathological studies and immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues.
34641376	8	31	theme	levels	1473:1478	arg1	studies					1411:1417	the histopathological studies	1389:1417	the histopathological studies	1389:1417	All the results demonstrated the significant inflammation reduction via the inflammatory cytokines (IL-1β and IL8), nitrosative (NO), and prostaglandin E2 (PGE2) supported by the histopathological studies and immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues.
34641376	8	31	theme	levels	1473:1478	arg1	assessment					1443:1452	immunohistochemical assessment	1423:1452	immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues	1423:1493	All the results demonstrated the significant inflammation reduction via the inflammatory cytokines (IL-1β and IL8), nitrosative (NO), and prostaglandin E2 (PGE2) supported by the histopathological studies and immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues.
34641376	7	32	theme	drugs	1200:1204	arg1	groups					1206:1211	the control and reference drugs groups	1174:1211	groups	1206:1211	The ABSEO and its nanoemulsion exhibited significant inflammation suppression in carrageenan-induced rat paw edema model, in both oral (50 and 100 mg/kg) and topical (5% in soyabean oil) routes, compared to the control and reference drugs groups.
34641376	7	33	theme	inflammation	1020:1031	arg1	suppression					1033:1043	significant inflammation suppression	1008:1043	significant inflammation suppression	1008:1043	The ABSEO and its nanoemulsion exhibited significant inflammation suppression in carrageenan-induced rat paw edema model, in both oral (50 and 100 mg/kg) and topical (5% in soyabean oil) routes, compared to the control and reference drugs groups.
34641376	9	34	theme	nanoemulsion	1547:1558	arg1	administration					1515:1528	the oral administration	1506:1528	the oral administration of ABSEO and its nanoemulsion (50 and 100 mg/kg)	1506:1577	Moreover, the oral administration of ABSEO and its nanoemulsion (50 and 100 mg/kg) exhibited antipyretic activity in rats, demonstrated by the inhibition of hyperthermia induced by intramuscular injection of brewer's yeast.
34641376	6	35	theme	16.21	873:877	arg1	α-pinene					863:870	α-pinene	863:870	α-pinene (16.21%)	863:879	The main compounds of the ABSEO were beyerene (20.81%), α-pinene (16.21%), D-limonene (14.22%), germacrene D (6.69%), β-humulene (4.14%), and sabinene (4.12%).
34641376	6	35	theme	16.21	873:877	arg1	%					878:878	16.21%	873:878	16.21%	873:878	The main compounds of the ABSEO were beyerene (20.81%), α-pinene (16.21%), D-limonene (14.22%), germacrene D (6.69%), β-humulene (4.14%), and sabinene (4.12%).
34641376	3	36	theme	A.	431:432	arg1	shoots					445:450	A. bidiwillii shoots EO (ABSEO)	431:461	A. bidiwillii shoots EO (ABSEO)	431:461	Herein, the chemical profile of A. bidiwillii shoots EO (ABSEO) was created by GC-MS analysis.
34641376	6	37	theme	germacrene	903:912	arg1	D					914:914	germacrene D	903:914	germacrene D (6.69%)	903:922	The main compounds of the ABSEO were beyerene (20.81%), α-pinene (16.21%), D-limonene (14.22%), germacrene D (6.69%), β-humulene (4.14%), and sabinene (4.12%).
34641376	6	37	theme	germacrene	903:912	arg1	%					921:921	6.69%	917:921	6.69%	917:921	The main compounds of the ABSEO were beyerene (20.81%), α-pinene (16.21%), D-limonene (14.22%), germacrene D (6.69%), β-humulene (4.14%), and sabinene (4.12%).
34641376	4	38	theme	in	512:513	arg1	effect					555:560	the in vivo oral and topical anti-inflammatory effect	508:560	the in vivo oral and topical anti-inflammatory effect against carrageenan-induced models, as well as antipyretic potentiality of ABSEO and its nanoemulsion	508:662	Additionally, the in vivo oral and topical anti-inflammatory effect against carrageenan-induced models, as well as antipyretic potentiality of ABSEO and its nanoemulsion were evaluated.
34641376	5	39	theme	Forty-three	680:690	arg1	components					702:711	Forty-three terpenoid components	680:711	Forty-three terpenoid components	680:711	Forty-three terpenoid components were identified and categorized as mono- (42.94%), sesqui- (31.66%), and diterpenes (23.74%).
34641376	5	39	theme	Forty-three	680:690	arg1	diterpenes					786:795	diterpenes	786:795	diterpenes (23.74%)	786:804	Forty-three terpenoid components were identified and categorized as mono- (42.94%), sesqui- (31.66%), and diterpenes (23.74%).
34641376	5	39	theme	Forty-three	680:690	arg1	mono-					748:752	mono-	748:752	mono- (42.94%)	748:761	Forty-three terpenoid components were identified and categorized as mono- (42.94%), sesqui- (31.66%), and diterpenes (23.74%).
34641376	5	39	theme	Forty-three	680:690	arg1	sesqui-					764:770	sesqui-	764:770	sesqui- (31.66%)	764:779	Forty-three terpenoid components were identified and categorized as mono- (42.94%), sesqui- (31.66%), and diterpenes (23.74%).
34641376	10	40	theme	ABSEO	1759:1763	arg1	nanoemulsion					1773:1784	its nanoemulsion	1769:1784	its nanoemulsion against numerous inflammatory and hyperthermia ailments that could be attributed to its active constituents	1769:1892	These findings advised that the use of ABSEO and its nanoemulsion against numerous inflammatory and hyperthermia ailments that could be attributed to its active constituents.
34641376	10	40	theme	ABSEO	1759:1763	arg1	use					1752:1754	the use	1748:1754	the use of ABSEO	1748:1763	These findings advised that the use of ABSEO and its nanoemulsion against numerous inflammatory and hyperthermia ailments that could be attributed to its active constituents.
34641376	10	41	theme	inflammatory	1803:1814	arg1	ailments					1833:1840	numerous inflammatory and hyperthermia ailments	1794:1840	numerous inflammatory and hyperthermia ailments that could be attributed to its active constituents	1794:1892	These findings advised that the use of ABSEO and its nanoemulsion against numerous inflammatory and hyperthermia ailments that could be attributed to its active constituents.
34641376	3	42	theme	shoots	445:450	arg1	profile					420:426	the chemical profile	407:426	the chemical profile of A. bidiwillii shoots EO (ABSEO)	407:461	Herein, the chemical profile of A. bidiwillii shoots EO (ABSEO) was created by GC-MS analysis.
34641376	7	43	theme	soyabean	1140:1147	arg1	oil					1149:1151	soyabean oil	1140:1151	soyabean oil	1140:1151	The ABSEO and its nanoemulsion exhibited significant inflammation suppression in carrageenan-induced rat paw edema model, in both oral (50 and 100 mg/kg) and topical (5% in soyabean oil) routes, compared to the control and reference drugs groups.
34641376	9	44	theme	antipyretic	1589:1599	arg1	activity					1601:1608	antipyretic activity	1589:1608	antipyretic activity in rats	1589:1616	Moreover, the oral administration of ABSEO and its nanoemulsion (50 and 100 mg/kg) exhibited antipyretic activity in rats, demonstrated by the inhibition of hyperthermia induced by intramuscular injection of brewer's yeast.
34641376	5	45	theme	terpenoid	692:700	arg1	components					702:711	Forty-three terpenoid components	680:711	Forty-three terpenoid components	680:711	Forty-three terpenoid components were identified and categorized as mono- (42.94%), sesqui- (31.66%), and diterpenes (23.74%).
34641376	5	45	theme	terpenoid	692:700	arg1	diterpenes					786:795	diterpenes	786:795	diterpenes (23.74%)	786:804	Forty-three terpenoid components were identified and categorized as mono- (42.94%), sesqui- (31.66%), and diterpenes (23.74%).
34641376	5	45	theme	terpenoid	692:700	arg1	mono-					748:752	mono-	748:752	mono- (42.94%)	748:761	Forty-three terpenoid components were identified and categorized as mono- (42.94%), sesqui- (31.66%), and diterpenes (23.74%).
34641376	5	45	theme	terpenoid	692:700	arg1	sesqui-					764:770	sesqui-	764:770	sesqui- (31.66%)	764:779	Forty-three terpenoid components were identified and categorized as mono- (42.94%), sesqui- (31.66%), and diterpenes (23.74%).
34641376	1	46	theme	bidiwillii	197:206	arg1	shoots					258:263	shoots	258:263	shoots	258:263	Different parts of Araucaria bidiwillii (bunya pin) trees, such as nuts, seeds, bark, and shoots, are widely used in cooking, tea, and traditional medicines around the world.
34641376	1	46	theme	bidiwillii	197:206	arg1	seeds					241:245	seeds	241:245	seeds	241:245	Different parts of Araucaria bidiwillii (bunya pin) trees, such as nuts, seeds, bark, and shoots, are widely used in cooking, tea, and traditional medicines around the world.
34641376	1	46	theme	bidiwillii	197:206	arg1	bark					248:251	bark	248:251	bark	248:251	Different parts of Araucaria bidiwillii (bunya pin) trees, such as nuts, seeds, bark, and shoots, are widely used in cooking, tea, and traditional medicines around the world.
34641376	1	46	theme	bidiwillii	197:206	arg1	nuts					235:238	nuts	235:238	nuts	235:238	Different parts of Araucaria bidiwillii (bunya pin) trees, such as nuts, seeds, bark, and shoots, are widely used in cooking, tea, and traditional medicines around the world.
34641376	1	46	theme	bidiwillii	197:206	arg1	trees					220:224	Araucaria bidiwillii (bunya pin) trees	187:224	Araucaria bidiwillii (bunya pin) trees	187:224	Different parts of Araucaria bidiwillii (bunya pin) trees, such as nuts, seeds, bark, and shoots, are widely used in cooking, tea, and traditional medicines around the world.
34641376	4	47	theme	carrageenan-induced	570:588	arg1	models					590:595	carrageenan-induced models	570:595	carrageenan-induced models	570:595	Additionally, the in vivo oral and topical anti-inflammatory effect against carrageenan-induced models, as well as antipyretic potentiality of ABSEO and its nanoemulsion were evaluated.
34641376	7	48	dep	oral	1097:1100	arg1	mg/kg					1114:1118	50 and 100 mg/kg	1103:1118	50 and 100 mg/kg	1103:1118	The ABSEO and its nanoemulsion exhibited significant inflammation suppression in carrageenan-induced rat paw edema model, in both oral (50 and 100 mg/kg) and topical (5% in soyabean oil) routes, compared to the control and reference drugs groups.
34641376	10	49	theme	hyperthermia	1820:1831	arg1	ailments					1833:1840	numerous inflammatory and hyperthermia ailments	1794:1840	numerous inflammatory and hyperthermia ailments that could be attributed to its active constituents	1794:1892	These findings advised that the use of ABSEO and its nanoemulsion against numerous inflammatory and hyperthermia ailments that could be attributed to its active constituents.
34641376	4	50	theme	oral	520:523	arg1	effect					555:560	the in vivo oral and topical anti-inflammatory effect	508:560	the in vivo oral and topical anti-inflammatory effect against carrageenan-induced models, as well as antipyretic potentiality of ABSEO and its nanoemulsion	508:662	Additionally, the in vivo oral and topical anti-inflammatory effect against carrageenan-induced models, as well as antipyretic potentiality of ABSEO and its nanoemulsion were evaluated.
34641376	0	51	theme	Chemical	146:153	arg1	Composition					155:165	Chemical Composition	146:165	Chemical Composition	146:165	Oral and Topical Anti-Inflammatory and Antipyretic Potentialities of Araucaria bidiwillii Shoot Essential Oil and Its Nanoemulsion in Relation to Chemical Composition.
34641376	8	52	dep	cytokines	1303:1311	arg1	IL8					1324:1326	IL8	1324:1326	IL8	1324:1326	All the results demonstrated the significant inflammation reduction via the inflammatory cytokines (IL-1β and IL8), nitrosative (NO), and prostaglandin E2 (PGE2) supported by the histopathological studies and immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues.
34641376	8	52	dep	cytokines	1303:1311	arg1	IL-1β					1314:1318	IL-1β	1314:1318	IL-1β	1314:1318	All the results demonstrated the significant inflammation reduction via the inflammatory cytokines (IL-1β and IL8), nitrosative (NO), and prostaglandin E2 (PGE2) supported by the histopathological studies and immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues.
34641376	8	52	dep	cytokines	1303:1311	arg1	cytokines					1303:1311	the inflammatory cytokines	1286:1311	the inflammatory cytokines (IL-1β and IL8)	1286:1327	All the results demonstrated the significant inflammation reduction via the inflammatory cytokines (IL-1β and IL8), nitrosative (NO), and prostaglandin E2 (PGE2) supported by the histopathological studies and immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues.
34641376	8	53	theme	immunohistochemical	1423:1441	arg1	assessment					1443:1452	immunohistochemical assessment	1423:1452	immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues	1423:1493	All the results demonstrated the significant inflammation reduction via the inflammatory cytokines (IL-1β and IL8), nitrosative (NO), and prostaglandin E2 (PGE2) supported by the histopathological studies and immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues.
34641376	8	54	from	levels	1473:1478	arg1	tissues					1487:1493	paw tissues	1483:1493	paw tissues	1483:1493	All the results demonstrated the significant inflammation reduction via the inflammatory cytokines (IL-1β and IL8), nitrosative (NO), and prostaglandin E2 (PGE2) supported by the histopathological studies and immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues.
34641376	3	55	theme	GC-MS	478:482	arg1	analysis					484:491	GC-MS analysis	478:491	GC-MS analysis	478:491	Herein, the chemical profile of A. bidiwillii shoots EO (ABSEO) was created by GC-MS analysis.
34641376	3	56	dep	shoots	445:450	arg1	ABSEO					456:460	ABSEO	456:460	ABSEO	456:460	Herein, the chemical profile of A. bidiwillii shoots EO (ABSEO) was created by GC-MS analysis.
34641376	3	56	dep	shoots	445:450	arg1	EO					452:453	EO	452:453	A. bidiwillii shoots EO (ABSEO)	431:461	Herein, the chemical profile of A. bidiwillii shoots EO (ABSEO) was created by GC-MS analysis.
34641376	0	57	from	Nanoemulsion	118:129	arg1	Relation					134:141	Relation	134:141	Relation to Chemical Composition	134:165	Oral and Topical Anti-Inflammatory and Antipyretic Potentialities of Araucaria bidiwillii Shoot Essential Oil and Its Nanoemulsion in Relation to Chemical Composition.
34641376	4	58	theme	antipyretic	609:619	arg1	potentiality					621:632	antipyretic potentiality	609:632	antipyretic potentiality of ABSEO	609:641	Additionally, the in vivo oral and topical anti-inflammatory effect against carrageenan-induced models, as well as antipyretic potentiality of ABSEO and its nanoemulsion were evaluated.
34641376	4	59	dep	in	512:513	arg1	vivo					515:518	vivo	515:518	vivo	515:518	Additionally, the in vivo oral and topical anti-inflammatory effect against carrageenan-induced models, as well as antipyretic potentiality of ABSEO and its nanoemulsion were evaluated.
34641376	9	60	from	activity	1601:1608	arg1	rats					1613:1616	rats	1613:1616	rats	1613:1616	Moreover, the oral administration of ABSEO and its nanoemulsion (50 and 100 mg/kg) exhibited antipyretic activity in rats, demonstrated by the inhibition of hyperthermia induced by intramuscular injection of brewer's yeast.
34641376	9	61	theme	hyperthermia	1653:1664	arg1	inhibition					1639:1648	the inhibition	1635:1648	the inhibition of hyperthermia induced by intramuscular injection of brewer's yeast	1635:1717	Moreover, the oral administration of ABSEO and its nanoemulsion (50 and 100 mg/kg) exhibited antipyretic activity in rats, demonstrated by the inhibition of hyperthermia induced by intramuscular injection of brewer's yeast.
34641376	0	62	theme	bidiwillii	79:88	arg1	Oil					106:108	Araucaria bidiwillii Shoot Essential Oil	69:108	Araucaria bidiwillii Shoot Essential Oil	69:108	Oral and Topical Anti-Inflammatory and Antipyretic Potentialities of Araucaria bidiwillii Shoot Essential Oil and Its Nanoemulsion in Relation to Chemical Composition.
34641376	1	63	theme	bunya	209:213	arg1	shoots					258:263	shoots	258:263	shoots	258:263	Different parts of Araucaria bidiwillii (bunya pin) trees, such as nuts, seeds, bark, and shoots, are widely used in cooking, tea, and traditional medicines around the world.
34641376	1	63	theme	bunya	209:213	arg1	seeds					241:245	seeds	241:245	seeds	241:245	Different parts of Araucaria bidiwillii (bunya pin) trees, such as nuts, seeds, bark, and shoots, are widely used in cooking, tea, and traditional medicines around the world.
34641376	1	63	theme	bunya	209:213	arg1	bark					248:251	bark	248:251	bark	248:251	Different parts of Araucaria bidiwillii (bunya pin) trees, such as nuts, seeds, bark, and shoots, are widely used in cooking, tea, and traditional medicines around the world.
34641376	1	63	theme	bunya	209:213	arg1	nuts					235:238	nuts	235:238	nuts	235:238	Different parts of Araucaria bidiwillii (bunya pin) trees, such as nuts, seeds, bark, and shoots, are widely used in cooking, tea, and traditional medicines around the world.
34641376	1	63	theme	bunya	209:213	arg1	trees					220:224	Araucaria bidiwillii (bunya pin) trees	187:224	Araucaria bidiwillii (bunya pin) trees	187:224	Different parts of Araucaria bidiwillii (bunya pin) trees, such as nuts, seeds, bark, and shoots, are widely used in cooking, tea, and traditional medicines around the world.
34641376	7	64	dep	topical	1125:1131	arg1	%					1135:1135	5%	1134:1135	5% in soyabean oil	1134:1151	The ABSEO and its nanoemulsion exhibited significant inflammation suppression in carrageenan-induced rat paw edema model, in both oral (50 and 100 mg/kg) and topical (5% in soyabean oil) routes, compared to the control and reference drugs groups.
34641376	7	65	from	%	1135:1135	arg1	oil					1149:1151	soyabean oil	1140:1151	soyabean oil	1140:1151	The ABSEO and its nanoemulsion exhibited significant inflammation suppression in carrageenan-induced rat paw edema model, in both oral (50 and 100 mg/kg) and topical (5% in soyabean oil) routes, compared to the control and reference drugs groups.
34641376	10	66	theme	active	1874:1879	arg1	constituents					1881:1892	its active constituents	1870:1892	its active constituents	1870:1892	These findings advised that the use of ABSEO and its nanoemulsion against numerous inflammatory and hyperthermia ailments that could be attributed to its active constituents.
34641376	0	67	theme	Essential	96:104	arg1	Oil					106:108	Araucaria bidiwillii Shoot Essential Oil	69:108	Araucaria bidiwillii Shoot Essential Oil	69:108	Oral and Topical Anti-Inflammatory and Antipyretic Potentialities of Araucaria bidiwillii Shoot Essential Oil and Its Nanoemulsion in Relation to Chemical Composition.
34641376	8	68	theme	paw	1483:1485	arg1	tissues					1487:1493	paw tissues	1483:1493	paw tissues	1483:1493	All the results demonstrated the significant inflammation reduction via the inflammatory cytokines (IL-1β and IL8), nitrosative (NO), and prostaglandin E2 (PGE2) supported by the histopathological studies and immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues.
34641376	1	69	theme	Different	168:176	arg1	parts					178:182	Different parts	168:182	Different parts of Araucaria bidiwillii (bunya pin) trees, such as nuts, seeds, bark, and shoots,	168:264	Different parts of Araucaria bidiwillii (bunya pin) trees, such as nuts, seeds, bark, and shoots, are widely used in cooking, tea, and traditional medicines around the world.
34641376	0	70	theme	Nanoemulsion	118:129	arg1	Potentialities					51:64	Oral and Topical Anti-Inflammatory and Antipyretic Potentialities	0:64	Oral and Topical Anti-Inflammatory and Antipyretic Potentialities of Araucaria bidiwillii Shoot Essential Oil and Its Nanoemulsion in Relation to Chemical Composition.	0:166	Oral and Topical Anti-Inflammatory and Antipyretic Potentialities of Araucaria bidiwillii Shoot Essential Oil and Its Nanoemulsion in Relation to Chemical Composition.
34641376	1	71	theme	traditional	303:313	arg1	medicines					315:323	traditional medicines	303:323	traditional medicines around the world	303:340	Different parts of Araucaria bidiwillii (bunya pin) trees, such as nuts, seeds, bark, and shoots, are widely used in cooking, tea, and traditional medicines around the world.
34641376	9	72	theme	yeast	1713:1717	arg1	injection					1691:1699	intramuscular injection	1677:1699	intramuscular injection of brewer's yeast	1677:1717	Moreover, the oral administration of ABSEO and its nanoemulsion (50 and 100 mg/kg) exhibited antipyretic activity in rats, demonstrated by the inhibition of hyperthermia induced by intramuscular injection of brewer's yeast.
34641376	8	73	theme	inflammation	1259:1270	arg1	reduction					1272:1280	the significant inflammation reduction	1243:1280	the significant inflammation reduction via the inflammatory cytokines (IL-1β and IL8)	1243:1327	All the results demonstrated the significant inflammation reduction via the inflammatory cytokines (IL-1β and IL8), nitrosative (NO), and prostaglandin E2 (PGE2) supported by the histopathological studies and immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues.
34641376	0	74	theme	Anti-Inflammatory	17:33	arg1	Potentialities					51:64	Oral and Topical Anti-Inflammatory and Antipyretic Potentialities	0:64	Oral and Topical Anti-Inflammatory and Antipyretic Potentialities of Araucaria bidiwillii Shoot Essential Oil and Its Nanoemulsion in Relation to Chemical Composition.	0:166	Oral and Topical Anti-Inflammatory and Antipyretic Potentialities of Araucaria bidiwillii Shoot Essential Oil and Its Nanoemulsion in Relation to Chemical Composition.
34641376	7	75	theme	paw	1072:1074	arg1	edema					1076:1080	carrageenan-induced rat paw edema	1048:1080	carrageenan-induced rat paw edema model	1048:1086	The ABSEO and its nanoemulsion exhibited significant inflammation suppression in carrageenan-induced rat paw edema model, in both oral (50 and 100 mg/kg) and topical (5% in soyabean oil) routes, compared to the control and reference drugs groups.
34641376	0	76	theme	Antipyretic	39:49	arg1	Potentialities					51:64	Oral and Topical Anti-Inflammatory and Antipyretic Potentialities	0:64	Oral and Topical Anti-Inflammatory and Antipyretic Potentialities of Araucaria bidiwillii Shoot Essential Oil and Its Nanoemulsion in Relation to Chemical Composition.	0:166	Oral and Topical Anti-Inflammatory and Antipyretic Potentialities of Araucaria bidiwillii Shoot Essential Oil and Its Nanoemulsion in Relation to Chemical Composition.
34641376	7	77	theme	carrageenan-induced	1048:1066	arg1	edema					1076:1080	carrageenan-induced rat paw edema	1048:1080	carrageenan-induced rat paw edema model	1048:1086	The ABSEO and its nanoemulsion exhibited significant inflammation suppression in carrageenan-induced rat paw edema model, in both oral (50 and 100 mg/kg) and topical (5% in soyabean oil) routes, compared to the control and reference drugs groups.
34641376	8	78	theme	inflammatory	1290:1301	arg1	IL8					1324:1326	IL8	1324:1326	IL8	1324:1326	All the results demonstrated the significant inflammation reduction via the inflammatory cytokines (IL-1β and IL8), nitrosative (NO), and prostaglandin E2 (PGE2) supported by the histopathological studies and immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues.
34641376	8	78	theme	inflammatory	1290:1301	arg1	IL-1β					1314:1318	IL-1β	1314:1318	IL-1β	1314:1318	All the results demonstrated the significant inflammation reduction via the inflammatory cytokines (IL-1β and IL8), nitrosative (NO), and prostaglandin E2 (PGE2) supported by the histopathological studies and immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues.
34641376	8	78	theme	inflammatory	1290:1301	arg1	cytokines					1303:1311	the inflammatory cytokines	1286:1311	the inflammatory cytokines (IL-1β and IL8)	1286:1327	All the results demonstrated the significant inflammation reduction via the inflammatory cytokines (IL-1β and IL8), nitrosative (NO), and prostaglandin E2 (PGE2) supported by the histopathological studies and immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues.
34641376	2	79	theme	shoots	347:352	arg1	EO					369:370	EO	369:370	EO	369:370	The shoots essential oil (EO) has not yet been studied.
34641376	2	79	theme	shoots	347:352	arg1	oil					364:366	The shoots essential oil	343:366	The shoots essential oil (EO)	343:371	The shoots essential oil (EO) has not yet been studied.
34641376	9	80	theme	ABSEO	1533:1537	arg1	administration					1515:1528	the oral administration	1506:1528	the oral administration of ABSEO and its nanoemulsion (50 and 100 mg/kg)	1506:1577	Moreover, the oral administration of ABSEO and its nanoemulsion (50 and 100 mg/kg) exhibited antipyretic activity in rats, demonstrated by the inhibition of hyperthermia induced by intramuscular injection of brewer's yeast.
34641376	3	81	theme	chemical	411:418	arg1	profile					420:426	the chemical profile	407:426	the chemical profile of A. bidiwillii shoots EO (ABSEO)	407:461	Herein, the chemical profile of A. bidiwillii shoots EO (ABSEO) was created by GC-MS analysis.
34641376	7	82	theme	significant	1008:1018	arg1	suppression					1033:1043	significant inflammation suppression	1008:1043	significant inflammation suppression	1008:1043	The ABSEO and its nanoemulsion exhibited significant inflammation suppression in carrageenan-induced rat paw edema model, in both oral (50 and 100 mg/kg) and topical (5% in soyabean oil) routes, compared to the control and reference drugs groups.
34641376	8	83	theme	prostaglandin	1352:1364	arg1	PGE2					1370:1373	PGE2	1370:1373	PGE2	1370:1373	All the results demonstrated the significant inflammation reduction via the inflammatory cytokines (IL-1β and IL8), nitrosative (NO), and prostaglandin E2 (PGE2) supported by the histopathological studies and immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues.
34641376	8	83	theme	prostaglandin	1352:1364	arg1	E2					1366:1367	prostaglandin E2	1352:1367	prostaglandin E2 (PGE2) supported by the histopathological studies and immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues	1352:1493	All the results demonstrated the significant inflammation reduction via the inflammatory cytokines (IL-1β and IL8), nitrosative (NO), and prostaglandin E2 (PGE2) supported by the histopathological studies and immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues.
34641376	4	84	theme	topical	529:535	arg1	effect					555:560	the in vivo oral and topical anti-inflammatory effect	508:560	the in vivo oral and topical anti-inflammatory effect against carrageenan-induced models, as well as antipyretic potentiality of ABSEO and its nanoemulsion	508:662	Additionally, the in vivo oral and topical anti-inflammatory effect against carrageenan-induced models, as well as antipyretic potentiality of ABSEO and its nanoemulsion were evaluated.
34641376	0	85	from	Relation	134:141	arg1	Potentialities					51:64	Oral and Topical Anti-Inflammatory and Antipyretic Potentialities	0:64	Oral and Topical Anti-Inflammatory and Antipyretic Potentialities of Araucaria bidiwillii Shoot Essential Oil and Its Nanoemulsion in Relation to Chemical Composition.	0:166	Oral and Topical Anti-Inflammatory and Antipyretic Potentialities of Araucaria bidiwillii Shoot Essential Oil and Its Nanoemulsion in Relation to Chemical Composition.
34641376	8	86	from	studies	1411:1417	arg1	tissues					1487:1493	paw tissues	1483:1493	paw tissues	1483:1493	All the results demonstrated the significant inflammation reduction via the inflammatory cytokines (IL-1β and IL8), nitrosative (NO), and prostaglandin E2 (PGE2) supported by the histopathological studies and immunohistochemical assessment of MMP-9 and NF-κβ levels in paw tissues.
34641376	3	87	theme	bidiwillii	434:443	arg1	shoots					445:450	A. bidiwillii shoots EO (ABSEO)	431:461	A. bidiwillii shoots EO (ABSEO)	431:461	Herein, the chemical profile of A. bidiwillii shoots EO (ABSEO) was created by GC-MS analysis.
32877217	0	0	theme	mouse	102:106	arg1	tendons					117:123	mouse Achilles tendons	102:123	mouse Achilles tendons	102:123	Single-cell transcriptomic analysis identifies extensive heterogeneity in the cellular composition of mouse Achilles tendons.
32877217	6	1	theme	inference	930:938	arg1	analysis					940:947	trajectory inference analysis	919:947	trajectory inference analysis	919:947	Using trajectory inference analysis, we provide additional support for the notion that pericytes are likely to be at least one of the progenitor cell populations for the fibroblasts that compose adult tendons.
32877217	4	2	theme	tendon	717:722	arg1	fibroblasts					724:734	tendon fibroblasts	717:734	tendon fibroblasts	717:734	In assembling a transcriptomic atlas of Achilles tendons, we identified 11 distinct types of cells, including three previously undescribed populations of tendon fibroblasts.
32877217	3	3	theme	mouse	506:510	arg1	tendons					521:527	mouse Achilles tendons	506:527	mouse Achilles tendons	506:527	To address this, we determined the heterogeneity of cell populations within mouse Achilles tendons using single-cell RNA sequencing.
32877217	9	4	theme	tendon	1475:1480	arg1	assembly					1503:1510	tendon extracellular matrix assembly	1475:1510	tendon extracellular matrix assembly	1475:1510	The atlas also serves as a resource to further the understanding of tendon extracellular matrix assembly and maintenance and in the design of therapies for tendinopathies.
32877217	7	5	theme	tendon	1251:1256	arg1	homeostasis					1258:1268	tendon homeostasis	1251:1268	tendon homeostasis	1251:1268	We also modeled cell-cell interactions and identified previously undescribed ligand-receptor signaling interactions involved in tendon homeostasis.
32877217	6	6	theme	trajectory	919:928	arg1	analysis					940:947	trajectory inference analysis	919:947	trajectory inference analysis	919:947	Using trajectory inference analysis, we provide additional support for the notion that pericytes are likely to be at least one of the progenitor cell populations for the fibroblasts that compose adult tendons.
32877217	3	7	theme	Achilles	512:519	arg1	tendons					521:527	mouse Achilles tendons	506:527	mouse Achilles tendons	506:527	To address this, we determined the heterogeneity of cell populations within mouse Achilles tendons using single-cell RNA sequencing.
32877217	8	8	theme	novel	1275:1279	arg1	atlas					1304:1308	Our novel and interactive tendon atlas	1271:1308	Our novel and interactive tendon atlas	1271:1308	Our novel and interactive tendon atlas highlights previously underappreciated heterogeneity between and within tendon cell populations.
32877217	0	9	theme	tendons	117:123	arg1	composition					87:97	the cellular composition	74:97	the cellular composition of mouse Achilles tendons	74:123	Single-cell transcriptomic analysis identifies extensive heterogeneity in the cellular composition of mouse Achilles tendons.
32877217	5	10	theme	tendon	893:898	arg1	fibroblasts					900:910	adult tendon fibroblasts	887:910	adult tendon fibroblasts	887:910	Prior studies have indicated that pericytes, which are found in the vasculature of tendons, could serve as a potential source of progenitor cells for adult tendon fibroblasts.
32877217	4	11	theme	tendons	612:618	arg1	atlas					594:598	a transcriptomic atlas	577:598	a transcriptomic atlas of Achilles tendons	577:618	In assembling a transcriptomic atlas of Achilles tendons, we identified 11 distinct types of cells, including three previously undescribed populations of tendon fibroblasts.
32877217	0	12	theme	Achilles	108:115	arg1	tendons					117:123	mouse Achilles tendons	102:123	mouse Achilles tendons	102:123	Single-cell transcriptomic analysis identifies extensive heterogeneity in the cellular composition of mouse Achilles tendons.
32877217	2	13	theme	biological	302:311	arg1	basis					313:317	the biological basis	298:317	the biological basis of tissue homeostasis and disease	298:351	Cellular heterogeneity is increasingly recognized as an important factor in the biological basis of tissue homeostasis and disease, yet little is known about the diversity of cell types that populate tendon.
32877217	9	14	theme	assembly	1503:1510	arg1	understanding					1458:1470	the understanding	1454:1470	the understanding of tendon extracellular matrix assembly and maintenance and in the design of therapies for tendinopathies	1454:1576	The atlas also serves as a resource to further the understanding of tendon extracellular matrix assembly and maintenance and in the design of therapies for tendinopathies.
32877217	2	15	theme	disease	345:351	arg1	basis					313:317	the biological basis	298:317	the biological basis of tissue homeostasis and disease	298:351	Cellular heterogeneity is increasingly recognized as an important factor in the biological basis of tissue homeostasis and disease, yet little is known about the diversity of cell types that populate tendon.
32877217	7	16	theme	signaling	1216:1224	arg1	interactions					1226:1237	previously undescribed ligand-receptor signaling interactions	1177:1237	previously undescribed ligand-receptor signaling interactions involved in tendon homeostasis	1177:1268	We also modeled cell-cell interactions and identified previously undescribed ligand-receptor signaling interactions involved in tendon homeostasis.
32877217	5	17	theme	progenitor	866:875	arg1	cells					877:881	progenitor cells	866:881	progenitor cells	866:881	Prior studies have indicated that pericytes, which are found in the vasculature of tendons, could serve as a potential source of progenitor cells for adult tendon fibroblasts.
32877217	3	18	theme	single-cell	535:545	arg1	sequencing					551:560	single-cell RNA sequencing	535:560	single-cell RNA sequencing	535:560	To address this, we determined the heterogeneity of cell populations within mouse Achilles tendons using single-cell RNA sequencing.
32877217	6	19	theme	populations	1063:1073	arg1	populations					1063:1073	the progenitor cell populations	1043:1073	the progenitor cell populations for the fibroblasts that compose adult tendons	1043:1120	Using trajectory inference analysis, we provide additional support for the notion that pericytes are likely to be at least one of the progenitor cell populations for the fibroblasts that compose adult tendons.
32877217	6	19	theme	populations	1063:1073	arg1	one					1036:1038	one	1036:1038	one	1036:1038	Using trajectory inference analysis, we provide additional support for the notion that pericytes are likely to be at least one of the progenitor cell populations for the fibroblasts that compose adult tendons.
32877217	2	20	theme	types	402:406	arg1	diversity					384:392	the diversity	380:392	the diversity of cell types that populate tendon	380:427	Cellular heterogeneity is increasingly recognized as an important factor in the biological basis of tissue homeostasis and disease, yet little is known about the diversity of cell types that populate tendon.
32877217	4	21	theme	Achilles	603:610	arg1	tendons					612:618	Achilles tendons	603:618	Achilles tendons	603:618	In assembling a transcriptomic atlas of Achilles tendons, we identified 11 distinct types of cells, including three previously undescribed populations of tendon fibroblasts.
32877217	0	22	from	heterogeneity	57:69	arg1	composition					87:97	the cellular composition	74:97	the cellular composition of mouse Achilles tendons	74:123	Single-cell transcriptomic analysis identifies extensive heterogeneity in the cellular composition of mouse Achilles tendons.
32877217	5	23	theme	tendons	820:826	arg1	vasculature					805:815	the vasculature	801:815	the vasculature of tendons	801:826	Prior studies have indicated that pericytes, which are found in the vasculature of tendons, could serve as a potential source of progenitor cells for adult tendon fibroblasts.
32877217	3	24	theme	RNA	547:549	arg1	sequencing					551:560	single-cell RNA sequencing	535:560	single-cell RNA sequencing	535:560	To address this, we determined the heterogeneity of cell populations within mouse Achilles tendons using single-cell RNA sequencing.
32877217	2	25	theme	cell	397:400	arg1	types					402:406	cell types	397:406	cell types that populate tendon	397:427	Cellular heterogeneity is increasingly recognized as an important factor in the biological basis of tissue homeostasis and disease, yet little is known about the diversity of cell types that populate tendon.
32877217	0	26	theme	transcriptomic	12:25	arg1	analysis					27:34	Single-cell transcriptomic analysis	0:34	Single-cell transcriptomic analysis	0:34	Single-cell transcriptomic analysis identifies extensive heterogeneity in the cellular composition of mouse Achilles tendons.
32877217	7	27	theme	undescribed	1188:1198	arg1	interactions					1226:1237	previously undescribed ligand-receptor signaling interactions	1177:1237	previously undescribed ligand-receptor signaling interactions involved in tendon homeostasis	1177:1268	We also modeled cell-cell interactions and identified previously undescribed ligand-receptor signaling interactions involved in tendon homeostasis.
32877217	5	28	theme	cells	877:881	arg1	source					856:861	a potential source	844:861	a potential source of progenitor cells for adult tendon fibroblasts	844:910	Prior studies have indicated that pericytes, which are found in the vasculature of tendons, could serve as a potential source of progenitor cells for adult tendon fibroblasts.
32877217	5	28	theme	cells	877:881	arg1	pericytes					771:779	pericytes	771:779	pericytes	771:779	Prior studies have indicated that pericytes, which are found in the vasculature of tendons, could serve as a potential source of progenitor cells for adult tendon fibroblasts.
32877217	0	29	theme	Single-cell	0:10	arg1	analysis					27:34	Single-cell transcriptomic analysis	0:34	Single-cell transcriptomic analysis	0:34	Single-cell transcriptomic analysis identifies extensive heterogeneity in the cellular composition of mouse Achilles tendons.
32877217	8	30	theme	cell	1389:1392	arg1	populations					1394:1404	tendon cell populations	1382:1404	tendon cell populations	1382:1404	Our novel and interactive tendon atlas highlights previously underappreciated heterogeneity between and within tendon cell populations.
32877217	9	31	theme	matrix	1496:1501	arg1	assembly					1503:1510	tendon extracellular matrix assembly	1475:1510	tendon extracellular matrix assembly	1475:1510	The atlas also serves as a resource to further the understanding of tendon extracellular matrix assembly and maintenance and in the design of therapies for tendinopathies.
32877217	2	32	theme	homeostasis	329:339	arg1	basis					313:317	the biological basis	298:317	the biological basis of tissue homeostasis and disease	298:351	Cellular heterogeneity is increasingly recognized as an important factor in the biological basis of tissue homeostasis and disease, yet little is known about the diversity of cell types that populate tendon.
32877217	6	33	theme	cell	1058:1061	arg1	populations					1063:1073	the progenitor cell populations	1043:1073	the progenitor cell populations for the fibroblasts that compose adult tendons	1043:1120	Using trajectory inference analysis, we provide additional support for the notion that pericytes are likely to be at least one of the progenitor cell populations for the fibroblasts that compose adult tendons.
32877217	2	34	theme	tissue	322:327	arg1	homeostasis					329:339	tissue homeostasis	322:339	tissue homeostasis	322:339	Cellular heterogeneity is increasingly recognized as an important factor in the biological basis of tissue homeostasis and disease, yet little is known about the diversity of cell types that populate tendon.
32877217	6	35	theme	progenitor	1047:1056	arg1	populations					1063:1073	the progenitor cell populations	1043:1073	the progenitor cell populations for the fibroblasts that compose adult tendons	1043:1120	Using trajectory inference analysis, we provide additional support for the notion that pericytes are likely to be at least one of the progenitor cell populations for the fibroblasts that compose adult tendons.
32877217	4	36	theme	transcriptomic	579:592	arg1	atlas					594:598	a transcriptomic atlas	577:598	a transcriptomic atlas of Achilles tendons	577:618	In assembling a transcriptomic atlas of Achilles tendons, we identified 11 distinct types of cells, including three previously undescribed populations of tendon fibroblasts.
32877217	2	37	from	factor	288:293	arg1	basis					313:317	the biological basis	298:317	the biological basis of tissue homeostasis and disease	298:351	Cellular heterogeneity is increasingly recognized as an important factor in the biological basis of tissue homeostasis and disease, yet little is known about the diversity of cell types that populate tendon.
32877217	4	38	theme	distinct	638:645	arg1	types					647:651	11 distinct types	635:651	11 distinct types	635:651	In assembling a transcriptomic atlas of Achilles tendons, we identified 11 distinct types of cells, including three previously undescribed populations of tendon fibroblasts.
32877217	4	38	theme	distinct	638:645	arg1	populations					702:712	three previously undescribed populations	673:712	three previously undescribed populations of tendon fibroblasts	673:734	In assembling a transcriptomic atlas of Achilles tendons, we identified 11 distinct types of cells, including three previously undescribed populations of tendon fibroblasts.
32877217	5	39	theme	Prior	737:741	arg1	studies					743:749	Prior studies	737:749	Prior studies	737:749	Prior studies have indicated that pericytes, which are found in the vasculature of tendons, could serve as a potential source of progenitor cells for adult tendon fibroblasts.
32877217	5	40	theme	potential	846:854	arg1	source					856:861	a potential source	844:861	a potential source of progenitor cells for adult tendon fibroblasts	844:910	Prior studies have indicated that pericytes, which are found in the vasculature of tendons, could serve as a potential source of progenitor cells for adult tendon fibroblasts.
32877217	5	40	theme	potential	846:854	arg1	pericytes					771:779	pericytes	771:779	pericytes	771:779	Prior studies have indicated that pericytes, which are found in the vasculature of tendons, could serve as a potential source of progenitor cells for adult tendon fibroblasts.
32877217	3	41	gly	heterogeneity	465:477	arg1	populations					487:497	cell populations	482:497	cell populations	482:497	To address this, we determined the heterogeneity of cell populations within mouse Achilles tendons using single-cell RNA sequencing.
32877217	8	42	theme	interactive	1285:1295	arg1	atlas					1304:1308	Our novel and interactive tendon atlas	1271:1308	Our novel and interactive tendon atlas	1271:1308	Our novel and interactive tendon atlas highlights previously underappreciated heterogeneity between and within tendon cell populations.
32877217	4	43	theme	cells	656:660	arg1	types					647:651	11 distinct types	635:651	11 distinct types	635:651	In assembling a transcriptomic atlas of Achilles tendons, we identified 11 distinct types of cells, including three previously undescribed populations of tendon fibroblasts.
32877217	4	43	theme	cells	656:660	arg1	populations					702:712	three previously undescribed populations	673:712	three previously undescribed populations of tendon fibroblasts	673:734	In assembling a transcriptomic atlas of Achilles tendons, we identified 11 distinct types of cells, including three previously undescribed populations of tendon fibroblasts.
32877217	0	44	theme	extensive	47:55	arg1	heterogeneity					57:69	extensive heterogeneity	47:69	extensive heterogeneity in the cellular composition of mouse Achilles tendons	47:123	Single-cell transcriptomic analysis identifies extensive heterogeneity in the cellular composition of mouse Achilles tendons.
32877217	6	45	theme	additional	961:970	arg1	support					972:978	additional support	961:978	additional support for the notion that pericytes are likely to be at least one of the progenitor cell populations for the fibroblasts that compose adult tendons	961:1120	Using trajectory inference analysis, we provide additional support for the notion that pericytes are likely to be at least one of the progenitor cell populations for the fibroblasts that compose adult tendons.
32877217	9	46	theme	extracellular	1482:1494	arg1	assembly					1503:1510	tendon extracellular matrix assembly	1475:1510	tendon extracellular matrix assembly	1475:1510	The atlas also serves as a resource to further the understanding of tendon extracellular matrix assembly and maintenance and in the design of therapies for tendinopathies.
32877217	8	47	theme	tendon	1382:1387	arg1	populations					1394:1404	tendon cell populations	1382:1404	tendon cell populations	1382:1404	Our novel and interactive tendon atlas highlights previously underappreciated heterogeneity between and within tendon cell populations.
32877217	9	48	theme	therapies	1549:1557	arg1	design					1539:1544	the design	1535:1544	the design of therapies for tendinopathies	1535:1576	The atlas also serves as a resource to further the understanding of tendon extracellular matrix assembly and maintenance and in the design of therapies for tendinopathies.
32877217	9	49	from	understanding	1458:1470	arg1	design					1539:1544	the design	1535:1544	the design of therapies for tendinopathies	1535:1576	The atlas also serves as a resource to further the understanding of tendon extracellular matrix assembly and maintenance and in the design of therapies for tendinopathies.
32877217	6	50	theme	adult	1108:1112	arg1	tendons					1114:1120	adult tendons	1108:1120	adult tendons	1108:1120	Using trajectory inference analysis, we provide additional support for the notion that pericytes are likely to be at least one of the progenitor cell populations for the fibroblasts that compose adult tendons.
32877217	3	51	theme	populations	487:497	arg1	heterogeneity					465:477	the heterogeneity	461:477	the heterogeneity of cell populations within mouse Achilles tendons	461:527	To address this, we determined the heterogeneity of cell populations within mouse Achilles tendons using single-cell RNA sequencing.
32877217	7	52	theme	cell-cell	1139:1147	arg1	interactions					1149:1160	cell-cell interactions	1139:1160	cell-cell interactions	1139:1160	We also modeled cell-cell interactions and identified previously undescribed ligand-receptor signaling interactions involved in tendon homeostasis.
32877217	2	53	theme	Cellular	222:229	arg1	heterogeneity					231:243	Cellular heterogeneity	222:243	Cellular heterogeneity	222:243	Cellular heterogeneity is increasingly recognized as an important factor in the biological basis of tissue homeostasis and disease, yet little is known about the diversity of cell types that populate tendon.
32877217	2	53	theme	Cellular	222:229	arg1	factor					288:293	an important factor	275:293	an important factor in the biological basis of tissue homeostasis and disease	275:351	Cellular heterogeneity is increasingly recognized as an important factor in the biological basis of tissue homeostasis and disease, yet little is known about the diversity of cell types that populate tendon.
32877217	5	54	located	found	792:796	arg1	vasculature					805:815	the vasculature	801:815	the vasculature of tendons	801:826	Prior studies have indicated that pericytes, which are found in the vasculature of tendons, could serve as a potential source of progenitor cells for adult tendon fibroblasts.
32877217	5	54	located	found	792:796	arg2	source					856:861	a potential source	844:861	a potential source of progenitor cells for adult tendon fibroblasts	844:910	Prior studies have indicated that pericytes, which are found in the vasculature of tendons, could serve as a potential source of progenitor cells for adult tendon fibroblasts.
32877217	5	54	located	found	792:796	arg2	pericytes					771:779	pericytes	771:779	pericytes	771:779	Prior studies have indicated that pericytes, which are found in the vasculature of tendons, could serve as a potential source of progenitor cells for adult tendon fibroblasts.
32877217	4	55	theme	fibroblasts	724:734	arg1	populations					702:712	three previously undescribed populations	673:712	three previously undescribed populations of tendon fibroblasts	673:734	In assembling a transcriptomic atlas of Achilles tendons, we identified 11 distinct types of cells, including three previously undescribed populations of tendon fibroblasts.
32877217	8	56	theme	underappreciated	1332:1347	arg1	heterogeneity					1349:1361	previously underappreciated heterogeneity	1321:1361	previously underappreciated heterogeneity between and within tendon cell populations	1321:1404	Our novel and interactive tendon atlas highlights previously underappreciated heterogeneity between and within tendon cell populations.
32877217	8	57	theme	tendon	1297:1302	arg1	atlas					1304:1308	Our novel and interactive tendon atlas	1271:1308	Our novel and interactive tendon atlas	1271:1308	Our novel and interactive tendon atlas highlights previously underappreciated heterogeneity between and within tendon cell populations.
32877217	1	58	theme	dense	138:142	arg1	tissue					155:160	a dense connective tissue	136:160	a dense connective tissue that stores	136:172	Tendon is a dense connective tissue that stores and transmits forces between muscles and bones.
32877217	1	58	theme	dense	138:142	arg1	Tendon					126:131	Tendon	126:131	Tendon	126:131	Tendon is a dense connective tissue that stores and transmits forces between muscles and bones.
32877217	2	59	theme	important	278:286	arg1	heterogeneity					231:243	Cellular heterogeneity	222:243	Cellular heterogeneity	222:243	Cellular heterogeneity is increasingly recognized as an important factor in the biological basis of tissue homeostasis and disease, yet little is known about the diversity of cell types that populate tendon.
32877217	2	59	theme	important	278:286	arg1	factor					288:293	an important factor	275:293	an important factor in the biological basis of tissue homeostasis and disease	275:351	Cellular heterogeneity is increasingly recognized as an important factor in the biological basis of tissue homeostasis and disease, yet little is known about the diversity of cell types that populate tendon.
32877217	1	60	theme	connective	144:153	arg1	tissue					155:160	a dense connective tissue	136:160	a dense connective tissue that stores	136:172	Tendon is a dense connective tissue that stores and transmits forces between muscles and bones.
32877217	1	60	theme	connective	144:153	arg1	Tendon					126:131	Tendon	126:131	Tendon	126:131	Tendon is a dense connective tissue that stores and transmits forces between muscles and bones.
32877217	5	61	theme	adult	887:891	arg1	fibroblasts					900:910	adult tendon fibroblasts	887:910	adult tendon fibroblasts	887:910	Prior studies have indicated that pericytes, which are found in the vasculature of tendons, could serve as a potential source of progenitor cells for adult tendon fibroblasts.
32877217	3	62	theme	cell	482:485	arg1	populations					487:497	cell populations	482:497	cell populations	482:497	To address this, we determined the heterogeneity of cell populations within mouse Achilles tendons using single-cell RNA sequencing.
32877217	0	63	theme	cellular	78:85	arg1	composition					87:97	the cellular composition	74:97	the cellular composition of mouse Achilles tendons	74:123	Single-cell transcriptomic analysis identifies extensive heterogeneity in the cellular composition of mouse Achilles tendons.
32877217	4	64	theme	undescribed	690:700	arg1	populations					702:712	three previously undescribed populations	673:712	three previously undescribed populations of tendon fibroblasts	673:734	In assembling a transcriptomic atlas of Achilles tendons, we identified 11 distinct types of cells, including three previously undescribed populations of tendon fibroblasts.
32877217	7	65	theme	ligand-receptor	1200:1214	arg1	interactions					1226:1237	previously undescribed ligand-receptor signaling interactions	1177:1237	previously undescribed ligand-receptor signaling interactions involved in tendon homeostasis	1177:1268	We also modeled cell-cell interactions and identified previously undescribed ligand-receptor signaling interactions involved in tendon homeostasis.
34968462	7	0	theme	cell	1104:1107	arg1	apoptosis					1109:1117	colonic cell apoptosis	1096:1117	colonic cell apoptosis	1096:1117	As a result, we found that LB preserved the integrity of the colonic barrier by inhibiting colonic cell apoptosis and protecting the expression of occludin, claudin-1, and ZO-1.
34968462	6	1	from	microflora	936:945	arg1	attempt					953:959	an attempt to explore the mechanisms of LB against UC	950:1002	an attempt to explore the mechanisms of LB against UC	950:1002	We also investigated the composition of the intestinal barrier and microflora in an attempt to explore the mechanisms of LB against UC.
34968462	8	2	theme	microflora	1209:1218	arg1	composition					1220:1230	the microflora composition	1205:1230	the microflora composition	1205:1230	Moreover, LB reshaped the microflora composition by suppressing harmful bacteria (Enterococcus et al.) and boosting beneficial microorganisms (Bacteroides et al.).
34968462	1	3	theme	disease	243:249	arg1	worldwide					251:259	a major inflammatory disease worldwide	222:259	a major inflammatory disease worldwide	222:259	Ulcerative colitis (UC) is a major inflammatory disease worldwide.
34968462	1	3	theme	disease	243:249	arg1	colitis					206:212	Ulcerative colitis	195:212	Ulcerative colitis (UC)	195:217	Ulcerative colitis (UC) is a major inflammatory disease worldwide.
34968462	5	4	theme	DSS-induced	707:717	arg1	loss					726:729	DSS-induced weight loss	707:729	DSS-induced weight loss	707:729	Our results demonstrated that high dose LB (120 mg/kg) significantly prevented DSS-induced weight loss, disease activity index (DAI) increase, histological damage, and colonic inflammation, indicating that LB has ameliorative effects on UC.
34968462	0	5	theme	intestinal	172:181	arg1	microflora					183:192	intestinal microflora	172:192	intestinal microflora	172:192	Licoflavone B, an isoprene flavonoid derived from licorice residue, relieves dextran sodium sulfate-induced ulcerative colitis by rebuilding the gut barrier and regulating intestinal microflora.
34968462	6	6	theme	intestinal	913:922	arg1	barrier					924:930	the intestinal barrier	909:930	the intestinal barrier	909:930	We also investigated the composition of the intestinal barrier and microflora in an attempt to explore the mechanisms of LB against UC.
34968462	5	7	theme	dose	663:666	arg1	120 mg/kg					672:680	120 mg/kg	672:680	120 mg/kg	672:680	Our results demonstrated that high dose LB (120 mg/kg) significantly prevented DSS-induced weight loss, disease activity index (DAI) increase, histological damage, and colonic inflammation, indicating that LB has ameliorative effects on UC.
34968462	5	7	theme	dose	663:666	arg1	LB					668:669	high dose LB	658:669	high dose LB (120 mg/kg)	658:681	Our results demonstrated that high dose LB (120 mg/kg) significantly prevented DSS-induced weight loss, disease activity index (DAI) increase, histological damage, and colonic inflammation, indicating that LB has ameliorative effects on UC.
34968462	4	8	theme	current	486:492	arg1	study					494:498	the current study	482:498	the current study	482:498	In the current study, we separated licoflavone B (LB) from LFs and administered it to dextran sodium sulfate (DSS)-exposed C57BL/6 mice for 14 days.
34968462	2	9	theme	licorice	294:301	arg1	flavones					311:318	licorice residue flavones	294:318	licorice residue flavones (LFs)	294:324	We previously demonstrated that licorice residue flavones (LFs) showed satisfactory efficacy in the treatment of UC.
34968462	2	9	theme	licorice	294:301	arg1	LFs					321:323	LFs	321:323	LFs	321:323	We previously demonstrated that licorice residue flavones (LFs) showed satisfactory efficacy in the treatment of UC.
34968462	11	10	theme	anti-UC	1612:1618	arg1	agents					1620:1625	novel anti-UC agents	1606:1625	novel anti-UC agents	1606:1625	These results will provide valuable clues for the discovery of novel anti-UC agents.
34968462	7	11	theme	colonic	1096:1102	arg1	apoptosis					1109:1117	colonic cell apoptosis	1096:1117	colonic cell apoptosis	1096:1117	As a result, we found that LB preserved the integrity of the colonic barrier by inhibiting colonic cell apoptosis and protecting the expression of occludin, claudin-1, and ZO-1.
34968462	9	12	theme	MAPK	1439:1442	arg1	pathway					1444:1450	the MAPK pathway	1435:1450	the MAPK pathway	1435:1450	Further molecular exploration implied that LB exerted anti-UC activity through blocking the MAPK pathway.
34968462	8	13	dep	bacteria	1255:1262	arg1	al.					1281:1283	Enterococcus et al.	1265:1283	Enterococcus et al.	1265:1283	Moreover, LB reshaped the microflora composition by suppressing harmful bacteria (Enterococcus et al.) and boosting beneficial microorganisms (Bacteroides et al.).
34968462	7	14	theme	claudin-1	1162:1170	arg1	expression					1138:1147	the expression	1134:1147	the expression of occludin, claudin-1, and ZO-1	1134:1180	As a result, we found that LB preserved the integrity of the colonic barrier by inhibiting colonic cell apoptosis and protecting the expression of occludin, claudin-1, and ZO-1.
34968462	0	15	theme	ulcerative	108:117	arg1	colitis					119:125	dextran sodium sulfate-induced ulcerative colitis	77:125	dextran sodium sulfate-induced ulcerative colitis	77:125	Licoflavone B, an isoprene flavonoid derived from licorice residue, relieves dextran sodium sulfate-induced ulcerative colitis by rebuilding the gut barrier and regulating intestinal microflora.
34968462	8	16	dep	Bacteroides	1326:1336	arg1	al					1341:1342	Bacteroides et al	1326:1342	Bacteroides et al.	1326:1343	Moreover, LB reshaped the microflora composition by suppressing harmful bacteria (Enterococcus et al.) and boosting beneficial microorganisms (Bacteroides et al.).
34968462	9	17	theme	Further	1347:1353	arg1	exploration					1365:1375	Further molecular exploration	1347:1375	Further molecular exploration	1347:1375	Further molecular exploration implied that LB exerted anti-UC activity through blocking the MAPK pathway.
34968462	8	18	theme	harmful	1247:1253	arg1	bacteria					1255:1262	harmful bacteria	1247:1262	harmful bacteria (Enterococcus et al.)	1247:1284	Moreover, LB reshaped the microflora composition by suppressing harmful bacteria (Enterococcus et al.) and boosting beneficial microorganisms (Bacteroides et al.).
34968462	4	19	theme	C57BL/6	602:608	arg1	mice					610:613	dextran sodium sulfate (DSS)-exposed C57BL/6 mice	565:613	dextran sodium sulfate (DSS)-exposed C57BL/6 mice for 14 days	565:625	In the current study, we separated licoflavone B (LB) from LFs and administered it to dextran sodium sulfate (DSS)-exposed C57BL/6 mice for 14 days.
34968462	0	20	theme	sulfate-induced	92:106	arg1	colitis					119:125	dextran sodium sulfate-induced ulcerative colitis	77:125	dextran sodium sulfate-induced ulcerative colitis	77:125	Licoflavone B, an isoprene flavonoid derived from licorice residue, relieves dextran sodium sulfate-induced ulcerative colitis by rebuilding the gut barrier and regulating intestinal microflora.
34968462	6	21	theme	barrier	924:930	arg1	microflora					936:945	microflora	936:945	microflora in an attempt to explore the mechanisms of LB against UC	936:1002	We also investigated the composition of the intestinal barrier and microflora in an attempt to explore the mechanisms of LB against UC.
34968462	6	21	theme	barrier	924:930	arg1	composition					894:904	the composition	890:904	the composition of the intestinal barrier	890:930	We also investigated the composition of the intestinal barrier and microflora in an attempt to explore the mechanisms of LB against UC.
34968462	8	22	dep	microorganisms	1310:1323	arg1	Bacteroides					1326:1336	Bacteroides	1326:1336	Bacteroides	1326:1336	Moreover, LB reshaped the microflora composition by suppressing harmful bacteria (Enterococcus et al.) and boosting beneficial microorganisms (Bacteroides et al.).
34968462	5	23	theme	index	749:753	arg1	increase					761:768	disease activity index (DAI) increase	732:768	disease activity index (DAI) increase	732:768	Our results demonstrated that high dose LB (120 mg/kg) significantly prevented DSS-induced weight loss, disease activity index (DAI) increase, histological damage, and colonic inflammation, indicating that LB has ameliorative effects on UC.
34968462	4	24	theme	-exposed	593:600	arg1	mice					610:613	dextran sodium sulfate (DSS)-exposed C57BL/6 mice	565:613	dextran sodium sulfate (DSS)-exposed C57BL/6 mice for 14 days	565:625	In the current study, we separated licoflavone B (LB) from LFs and administered it to dextran sodium sulfate (DSS)-exposed C57BL/6 mice for 14 days.
34968462	5	25	theme	activity	740:747	arg1	index					749:753	disease activity index	732:753	disease activity index (DAI) increase	732:768	Our results demonstrated that high dose LB (120 mg/kg) significantly prevented DSS-induced weight loss, disease activity index (DAI) increase, histological damage, and colonic inflammation, indicating that LB has ameliorative effects on UC.
34968462	5	25	theme	activity	740:747	arg1	DAI					756:758	DAI	756:758	DAI	756:758	Our results demonstrated that high dose LB (120 mg/kg) significantly prevented DSS-induced weight loss, disease activity index (DAI) increase, histological damage, and colonic inflammation, indicating that LB has ameliorative effects on UC.
34968462	0	26	theme	Licoflavone	0:10	arg1	flavonoid					27:35	an isoprene flavonoid	15:35	an isoprene flavonoid derived from licorice residue	15:65	Licoflavone B, an isoprene flavonoid derived from licorice residue, relieves dextran sodium sulfate-induced ulcerative colitis by rebuilding the gut barrier and regulating intestinal microflora.
34968462	0	26	theme	Licoflavone	0:10	arg1	B					12:12	Licoflavone B	0:12	Licoflavone B	0:12	Licoflavone B, an isoprene flavonoid derived from licorice residue, relieves dextran sodium sulfate-induced ulcerative colitis by rebuilding the gut barrier and regulating intestinal microflora.
34968462	0	27	attach	derived	37:43	arg2	flavonoid					27:35	an isoprene flavonoid	15:35	an isoprene flavonoid derived from licorice residue	15:65	Licoflavone B, an isoprene flavonoid derived from licorice residue, relieves dextran sodium sulfate-induced ulcerative colitis by rebuilding the gut barrier and regulating intestinal microflora.
34968462	0	27	attach	derived	37:43	arg1	residue					59:65	licorice residue	50:65	licorice residue	50:65	Licoflavone B, an isoprene flavonoid derived from licorice residue, relieves dextran sodium sulfate-induced ulcerative colitis by rebuilding the gut barrier and regulating intestinal microflora.
34968462	0	27	attach	derived	37:43	arg2	B					12:12	Licoflavone B	0:12	Licoflavone B	0:12	Licoflavone B, an isoprene flavonoid derived from licorice residue, relieves dextran sodium sulfate-induced ulcerative colitis by rebuilding the gut barrier and regulating intestinal microflora.
34968462	3	28	theme	LFs	423:425	arg1	ingredients					408:418	the ingredients	404:418	the ingredients of LFs	404:425	Therefore, research into the ingredients of LFs may lead to the discovery of novel anti-UC targets.
34968462	8	29	dep	LB	1193:1194	arg1	reshaped					1196:1203	reshaped	1196:1203	LB reshaped the microflora composition by suppressing harmful bacteria (Enterococcus et al.) and boosting beneficial microorganisms (Bacteroides et al.)	1193:1344	Moreover, LB reshaped the microflora composition by suppressing harmful bacteria (Enterococcus et al.) and boosting beneficial microorganisms (Bacteroides et al.).
34968462	10	30	theme	anti-UC	1471:1477	arg1	activity					1479:1486	anti-UC activity	1471:1486	anti-UC activity of LB	1471:1492	Here, we explored anti-UC activity of LB for the first time and clarified its mechanisms.
34968462	2	31	theme	residue	303:309	arg1	flavones					311:318	licorice residue flavones	294:318	licorice residue flavones (LFs)	294:324	We previously demonstrated that licorice residue flavones (LFs) showed satisfactory efficacy in the treatment of UC.
34968462	2	31	theme	residue	303:309	arg1	LFs					321:323	LFs	321:323	LFs	321:323	We previously demonstrated that licorice residue flavones (LFs) showed satisfactory efficacy in the treatment of UC.
34968462	4	32	theme	sulfate	580:586	arg1	mice					610:613	dextran sodium sulfate (DSS)-exposed C57BL/6 mice	565:613	dextran sodium sulfate (DSS)-exposed C57BL/6 mice for 14 days	565:625	In the current study, we separated licoflavone B (LB) from LFs and administered it to dextran sodium sulfate (DSS)-exposed C57BL/6 mice for 14 days.
34968462	5	33	theme	weight	719:724	arg1	loss					726:729	DSS-induced weight loss	707:729	DSS-induced weight loss	707:729	Our results demonstrated that high dose LB (120 mg/kg) significantly prevented DSS-induced weight loss, disease activity index (DAI) increase, histological damage, and colonic inflammation, indicating that LB has ameliorative effects on UC.
34968462	6	34	from	composition	894:904	arg1	attempt					953:959	an attempt to explore the mechanisms of LB against UC	950:1002	an attempt to explore the mechanisms of LB against UC	950:1002	We also investigated the composition of the intestinal barrier and microflora in an attempt to explore the mechanisms of LB against UC.
34968462	7	35	theme	colonic	1066:1072	arg1	barrier					1074:1080	the colonic barrier	1062:1080	the colonic barrier	1062:1080	As a result, we found that LB preserved the integrity of the colonic barrier by inhibiting colonic cell apoptosis and protecting the expression of occludin, claudin-1, and ZO-1.
34968462	2	36	theme	UC	375:376	arg1	treatment					362:370	the treatment	358:370	the treatment of UC	358:376	We previously demonstrated that licorice residue flavones (LFs) showed satisfactory efficacy in the treatment of UC.
34968462	7	37	theme	ZO-1	1177:1180	arg1	expression					1138:1147	the expression	1134:1147	the expression of occludin, claudin-1, and ZO-1	1134:1180	As a result, we found that LB preserved the integrity of the colonic barrier by inhibiting colonic cell apoptosis and protecting the expression of occludin, claudin-1, and ZO-1.
34968462	0	38	theme	isoprene	18:25	arg1	flavonoid					27:35	an isoprene flavonoid	15:35	an isoprene flavonoid derived from licorice residue	15:65	Licoflavone B, an isoprene flavonoid derived from licorice residue, relieves dextran sodium sulfate-induced ulcerative colitis by rebuilding the gut barrier and regulating intestinal microflora.
34968462	0	38	theme	isoprene	18:25	arg1	B					12:12	Licoflavone B	0:12	Licoflavone B	0:12	Licoflavone B, an isoprene flavonoid derived from licorice residue, relieves dextran sodium sulfate-induced ulcerative colitis by rebuilding the gut barrier and regulating intestinal microflora.
34968462	5	39	theme	high	658:661	arg1	120 mg/kg					672:680	120 mg/kg	672:680	120 mg/kg	672:680	Our results demonstrated that high dose LB (120 mg/kg) significantly prevented DSS-induced weight loss, disease activity index (DAI) increase, histological damage, and colonic inflammation, indicating that LB has ameliorative effects on UC.
34968462	5	39	theme	high	658:661	arg1	LB					668:669	high dose LB	658:669	high dose LB (120 mg/kg)	658:681	Our results demonstrated that high dose LB (120 mg/kg) significantly prevented DSS-induced weight loss, disease activity index (DAI) increase, histological damage, and colonic inflammation, indicating that LB has ameliorative effects on UC.
34968462	4	40	theme	sodium	573:578	arg1	DSS					589:591	DSS	589:591	DSS	589:591	In the current study, we separated licoflavone B (LB) from LFs and administered it to dextran sodium sulfate (DSS)-exposed C57BL/6 mice for 14 days.
34968462	4	40	theme	sodium	573:578	arg1	sulfate					580:586	dextran sodium sulfate	565:586	dextran sodium sulfate (DSS)-exposed C57BL/6 mice for 14 days	565:625	In the current study, we separated licoflavone B (LB) from LFs and administered it to dextran sodium sulfate (DSS)-exposed C57BL/6 mice for 14 days.
34968462	5	41	theme	histological	771:782	arg1	damage					784:789	histological damage	771:789	histological damage	771:789	Our results demonstrated that high dose LB (120 mg/kg) significantly prevented DSS-induced weight loss, disease activity index (DAI) increase, histological damage, and colonic inflammation, indicating that LB has ameliorative effects on UC.
34968462	11	42	theme	valuable	1570:1577	arg1	clues					1579:1583	valuable clues	1570:1583	valuable clues for the discovery of novel anti-UC agents	1570:1625	These results will provide valuable clues for the discovery of novel anti-UC agents.
34968462	9	43	theme	molecular	1355:1363	arg1	exploration					1365:1375	Further molecular exploration	1347:1375	Further molecular exploration	1347:1375	Further molecular exploration implied that LB exerted anti-UC activity through blocking the MAPK pathway.
34968462	1	44	theme	Ulcerative	195:204	arg1	worldwide					251:259	a major inflammatory disease worldwide	222:259	a major inflammatory disease worldwide	222:259	Ulcerative colitis (UC) is a major inflammatory disease worldwide.
34968462	1	44	theme	Ulcerative	195:204	arg1	UC					215:216	UC	215:216	UC	215:216	Ulcerative colitis (UC) is a major inflammatory disease worldwide.
34968462	1	44	theme	Ulcerative	195:204	arg1	colitis					206:212	Ulcerative colitis	195:212	Ulcerative colitis (UC)	195:217	Ulcerative colitis (UC) is a major inflammatory disease worldwide.
34968462	3	45	theme	targets	470:476	arg1	discovery					443:451	the discovery	439:451	the discovery of novel anti-UC targets	439:476	Therefore, research into the ingredients of LFs may lead to the discovery of novel anti-UC targets.
34968462	5	46	theme	disease	732:738	arg1	index					749:753	disease activity index	732:753	disease activity index (DAI) increase	732:768	Our results demonstrated that high dose LB (120 mg/kg) significantly prevented DSS-induced weight loss, disease activity index (DAI) increase, histological damage, and colonic inflammation, indicating that LB has ameliorative effects on UC.
34968462	5	46	theme	disease	732:738	arg1	DAI					756:758	DAI	756:758	DAI	756:758	Our results demonstrated that high dose LB (120 mg/kg) significantly prevented DSS-induced weight loss, disease activity index (DAI) increase, histological damage, and colonic inflammation, indicating that LB has ameliorative effects on UC.
34968462	4	47	theme	licoflavone	514:524	arg1	LB					529:530	LB	529:530	LB	529:530	In the current study, we separated licoflavone B (LB) from LFs and administered it to dextran sodium sulfate (DSS)-exposed C57BL/6 mice for 14 days.
34968462	4	47	theme	licoflavone	514:524	arg1	B					526:526	licoflavone B	514:526	licoflavone B (LB)	514:531	In the current study, we separated licoflavone B (LB) from LFs and administered it to dextran sodium sulfate (DSS)-exposed C57BL/6 mice for 14 days.
34968462	0	48	theme	gut	145:147	arg1	barrier					149:155	the gut barrier	141:155	the gut barrier	141:155	Licoflavone B, an isoprene flavonoid derived from licorice residue, relieves dextran sodium sulfate-induced ulcerative colitis by rebuilding the gut barrier and regulating intestinal microflora.
34968462	7	49	dep	LB	1032:1033	arg1	preserved					1035:1043	preserved	1035:1043	LB preserved the integrity of the colonic barrier by inhibiting colonic cell apoptosis and protecting the expression of occludin, claudin-1, and ZO-1	1032:1180	As a result, we found that LB preserved the integrity of the colonic barrier by inhibiting colonic cell apoptosis and protecting the expression of occludin, claudin-1, and ZO-1.
34968462	5	50	contain	has	837:839	arg2	effects					854:860	ameliorative effects	841:860	ameliorative effects	841:860	Our results demonstrated that high dose LB (120 mg/kg) significantly prevented DSS-induced weight loss, disease activity index (DAI) increase, histological damage, and colonic inflammation, indicating that LB has ameliorative effects on UC.
34968462	5	50	contain	has	837:839	arg1	LB					834:835	LB	834:835	LB	834:835	Our results demonstrated that high dose LB (120 mg/kg) significantly prevented DSS-induced weight loss, disease activity index (DAI) increase, histological damage, and colonic inflammation, indicating that LB has ameliorative effects on UC.
34968462	2	51	theme	satisfactory	333:344	arg1	efficacy					346:353	satisfactory efficacy	333:353	satisfactory efficacy	333:353	We previously demonstrated that licorice residue flavones (LFs) showed satisfactory efficacy in the treatment of UC.
34968462	11	52	theme	novel	1606:1610	arg1	agents					1620:1625	novel anti-UC agents	1606:1625	novel anti-UC agents	1606:1625	These results will provide valuable clues for the discovery of novel anti-UC agents.
34968462	3	53	theme	novel	456:460	arg1	targets					470:476	novel anti-UC targets	456:476	novel anti-UC targets	456:476	Therefore, research into the ingredients of LFs may lead to the discovery of novel anti-UC targets.
34968462	5	54	theme	colonic	796:802	arg1	inflammation					804:815	colonic inflammation	796:815	colonic inflammation	796:815	Our results demonstrated that high dose LB (120 mg/kg) significantly prevented DSS-induced weight loss, disease activity index (DAI) increase, histological damage, and colonic inflammation, indicating that LB has ameliorative effects on UC.
34968462	8	55	theme	beneficial	1299:1308	arg1	microorganisms					1310:1323	beneficial microorganisms	1299:1323	beneficial microorganisms (Bacteroides et al.)	1299:1344	Moreover, LB reshaped the microflora composition by suppressing harmful bacteria (Enterococcus et al.) and boosting beneficial microorganisms (Bacteroides et al.).
34968462	3	56	theme	anti-UC	462:468	arg1	targets					470:476	novel anti-UC targets	456:476	novel anti-UC targets	456:476	Therefore, research into the ingredients of LFs may lead to the discovery of novel anti-UC targets.
34968462	5	57	theme	ameliorative	841:852	arg1	effects					854:860	ameliorative effects	841:860	ameliorative effects	841:860	Our results demonstrated that high dose LB (120 mg/kg) significantly prevented DSS-induced weight loss, disease activity index (DAI) increase, histological damage, and colonic inflammation, indicating that LB has ameliorative effects on UC.
34968462	10	58	theme	LB	1491:1492	arg1	activity					1479:1486	anti-UC activity	1471:1486	anti-UC activity of LB	1471:1492	Here, we explored anti-UC activity of LB for the first time and clarified its mechanisms.
34968462	11	59	theme	agents	1620:1625	arg1	discovery					1593:1601	the discovery	1589:1601	the discovery of novel anti-UC agents	1589:1625	These results will provide valuable clues for the discovery of novel anti-UC agents.
34968462	9	60	theme	anti-UC	1401:1407	arg1	activity					1409:1416	anti-UC activity	1401:1416	anti-UC activity	1401:1416	Further molecular exploration implied that LB exerted anti-UC activity through blocking the MAPK pathway.
34968462	6	61	theme	LB	990:991	arg1	mechanisms					976:985	the mechanisms	972:985	the mechanisms of LB against UC	972:1002	We also investigated the composition of the intestinal barrier and microflora in an attempt to explore the mechanisms of LB against UC.
34968462	1	62	theme	major	224:228	arg1	worldwide					251:259	a major inflammatory disease worldwide	222:259	a major inflammatory disease worldwide	222:259	Ulcerative colitis (UC) is a major inflammatory disease worldwide.
34968462	1	62	theme	major	224:228	arg1	colitis					206:212	Ulcerative colitis	195:212	Ulcerative colitis (UC)	195:217	Ulcerative colitis (UC) is a major inflammatory disease worldwide.
34968462	0	63	theme	licorice	50:57	arg1	residue					59:65	licorice residue	50:65	licorice residue	50:65	Licoflavone B, an isoprene flavonoid derived from licorice residue, relieves dextran sodium sulfate-induced ulcerative colitis by rebuilding the gut barrier and regulating intestinal microflora.
34968462	7	64	theme	barrier	1074:1080	arg1	integrity					1049:1057	the integrity	1045:1057	the integrity of the colonic barrier	1045:1080	As a result, we found that LB preserved the integrity of the colonic barrier by inhibiting colonic cell apoptosis and protecting the expression of occludin, claudin-1, and ZO-1.
34968462	4	65	theme	dextran	565:571	arg1	DSS					589:591	DSS	589:591	DSS	589:591	In the current study, we separated licoflavone B (LB) from LFs and administered it to dextran sodium sulfate (DSS)-exposed C57BL/6 mice for 14 days.
34968462	4	65	theme	dextran	565:571	arg1	sulfate					580:586	dextran sodium sulfate	565:586	dextran sodium sulfate (DSS)-exposed C57BL/6 mice for 14 days	565:625	In the current study, we separated licoflavone B (LB) from LFs and administered it to dextran sodium sulfate (DSS)-exposed C57BL/6 mice for 14 days.
34968462	7	66	theme	occludin	1152:1159	arg1	expression					1138:1147	the expression	1134:1147	the expression of occludin, claudin-1, and ZO-1	1134:1180	As a result, we found that LB preserved the integrity of the colonic barrier by inhibiting colonic cell apoptosis and protecting the expression of occludin, claudin-1, and ZO-1.
34968462	10	67	theme	first	1502:1506	arg1	time					1508:1511	the first time	1498:1511	the first time	1498:1511	Here, we explored anti-UC activity of LB for the first time and clarified its mechanisms.
34968462	1	68	theme	inflammatory	230:241	arg1	worldwide					251:259	a major inflammatory disease worldwide	222:259	a major inflammatory disease worldwide	222:259	Ulcerative colitis (UC) is a major inflammatory disease worldwide.
34968462	1	68	theme	inflammatory	230:241	arg1	colitis					206:212	Ulcerative colitis	195:212	Ulcerative colitis (UC)	195:217	Ulcerative colitis (UC) is a major inflammatory disease worldwide.
34231485	14	0	theme	tissue	1942:1947	arg1	damage					1949:1954	tissue damage	1942:1954	tissue damage	1942:1954	CONCLUSION ICA can improve intestinal flora abundance and composition of DSS-induced colitis mice, and inhibit tissue damage and inflammatory response through modulating the p-p65/p65 expression.
34231485	2	1	theme	intestinal	330:339	arg1	microflora					341:350	the intestinal microflora	326:350	the intestinal microflora of colitis mice	326:366	This study aimed to investigate the effect of icariin (ICA) on the intestinal microflora of colitis mice.
34231485	13	2	theme	colon	1816:1820	arg1	tissues					1822:1828	colon tissues	1816:1828	colon tissues	1816:1828	ICA significantly reduced the proportion and activity of Bacteroides, Helicobacteraceae, Turicibacter, and significantly increased that of beneficial microflora (Lactobacillus, Lachnospiraceae, Akkermansia), so as improved damages of colon tissues.
34231485	10	3	theme	significant	1339:1349	arg1	weight					1351:1356	significant weight	1339:1356	significant weight	1339:1356	Compared with the DSS group, mice in the ICA group demonstrated significant weight and colon length (P < .05).
34231485	4	4	theme	disease	621:627	arg1	scoring					638:644	disease activity scoring	621:644	disease activity scoring (considering weight-loss percentage, stool-shape change, and stool-bleeding scoring)	621:729	The severity of inflammation in DSS-induced colitis mice was evaluated using disease activity scoring (considering weight-loss percentage, stool-shape change, and stool-bleeding scoring).
34231485	9	5	theme	P	1178:1178	arg1	activity					1168:1175	disease activity	1160:1175	disease activity (P < .05)	1160:1185	RESULTS Compared with DSS group, ICA significantly improved disease activity (P < .05) and reduced inflammatory damage of colon tissues (P < .05) in DSS-induced colitis mice.
34231485	9	5	theme	P	1178:1178	arg1	<					1180:1180	P < .05	1178:1184	P < .05	1178:1184	RESULTS Compared with DSS group, ICA significantly improved disease activity (P < .05) and reduced inflammatory damage of colon tissues (P < .05) in DSS-induced colitis mice.
34231485	2	6	theme	icariin	309:315	arg1	effect					299:304	the effect	295:304	the effect of icariin (ICA) on the intestinal microflora of colitis mice	295:366	This study aimed to investigate the effect of icariin (ICA) on the intestinal microflora of colitis mice.
34231485	1	7	theme	bowel	207:211	arg1	IBD					222:224	IBD	222:224	IBD	222:224	BACKGROUND Ulcerative colitis, as a kind of inflammatory bowel disease (IBD) is characterized by abdominal pain.
34231485	1	7	theme	bowel	207:211	arg1	disease					213:219	inflammatory bowel disease	194:219	inflammatory bowel disease (IBD)	194:225	BACKGROUND Ulcerative colitis, as a kind of inflammatory bowel disease (IBD) is characterized by abdominal pain.
34231485	4	8	theme	DSS-induced	576:586	arg1	colitis					588:594	DSS-induced colitis	576:594	DSS-induced colitis mice	576:599	The severity of inflammation in DSS-induced colitis mice was evaluated using disease activity scoring (considering weight-loss percentage, stool-shape change, and stool-bleeding scoring).
34231485	3	9	theme	ICA	513:515	arg1	DSS+ICA					528:534	DSS+ICA	528:534	DSS+ICA	528:534	METHODS Fifteen female C57BL/6 mice were randomly divided into the Control group, dextran sodium sulfate (DSS)-induced colitis (DSS) group, and ICA treatment (DSS+ICA) group.
34231485	3	9	theme	ICA	513:515	arg1	treatment					517:525	ICA treatment	513:525	ICA treatment (DSS+ICA) group	513:541	METHODS Fifteen female C57BL/6 mice were randomly divided into the Control group, dextran sodium sulfate (DSS)-induced colitis (DSS) group, and ICA treatment (DSS+ICA) group.
34231485	12	10	theme	p65	1488:1490	arg1	ratio					1492:1496	p-p65/ p65 ratio	1481:1496	p-p65/ p65 ratio in the DSS + ICA group	1481:1519	p-p65/ p65 ratio in the DSS + ICA group was remarkably enhanced compared to the DSS group (P < .05).
34231485	9	11	theme	inflammatory	1199:1210	arg1	damage					1212:1217	inflammatory damage	1199:1217	inflammatory damage of colon tissues (P < .05) in DSS-induced colitis mice	1199:1272	RESULTS Compared with DSS group, ICA significantly improved disease activity (P < .05) and reduced inflammatory damage of colon tissues (P < .05) in DSS-induced colitis mice.
34231485	6	12	theme	immunosorbent	896:908	arg1	assay					910:914	enzyme-linked immunosorbent assay	882:914	enzyme-linked immunosorbent assay	882:914	Serum levels of TNF-α and IL-6 were detected with enzyme-linked immunosorbent assay.
34231485	4	13	theme	stool-shape	683:693	arg1	change					695:700	stool-shape change	683:700	stool-shape change	683:700	The severity of inflammation in DSS-induced colitis mice was evaluated using disease activity scoring (considering weight-loss percentage, stool-shape change, and stool-bleeding scoring).
34231485	1	14	theme	Ulcerative	161:170	arg1	colitis					172:178	BACKGROUND Ulcerative colitis	150:178	BACKGROUND Ulcerative colitis	150:178	BACKGROUND Ulcerative colitis, as a kind of inflammatory bowel disease (IBD) is characterized by abdominal pain.
34231485	12	15	theme	P	1572:1572	arg1	group					1565:1569	the DSS group	1557:1569	the DSS group (P < .05)	1557:1579	p-p65/ p65 ratio in the DSS + ICA group was remarkably enhanced compared to the DSS group (P < .05).
34231485	12	15	theme	P	1572:1572	arg1	<					1574:1574	P < .05	1572:1578	P < .05	1572:1578	p-p65/ p65 ratio in the DSS + ICA group was remarkably enhanced compared to the DSS group (P < .05).
34231485	9	16	theme	tissues	1228:1234	arg1	damage					1212:1217	inflammatory damage	1199:1217	inflammatory damage of colon tissues (P < .05) in DSS-induced colitis mice	1199:1272	RESULTS Compared with DSS group, ICA significantly improved disease activity (P < .05) and reduced inflammatory damage of colon tissues (P < .05) in DSS-induced colitis mice.
34231485	11	17	theme	IL-6	1429:1432	arg1	expressions					1414:1424	expressions	1414:1424	expressions of IL-6 and TNF-α	1414:1442	ICA significantly inhibited expressions of IL-6 and TNF-α compared to the DSS group (P < .05).
34231485	0	18	theme	Intestinal	88:97	arg1	Abundance					105:113	Intestinal Flora Abundance	88:113	Intestinal Flora Abundance	88:113	Icariin Inhibits Intestinal Inflammation of DSS-Induced Colitis Mice Through Modulating Intestinal Flora Abundance and Modulating p-p65/p65 Molecule.
34231485	9	19	theme	P	1237:1237	arg1	tissues					1228:1234	colon tissues	1222:1234	colon tissues (P < .05)	1222:1244	RESULTS Compared with DSS group, ICA significantly improved disease activity (P < .05) and reduced inflammatory damage of colon tissues (P < .05) in DSS-induced colitis mice.
34231485	9	19	theme	P	1237:1237	arg1	<					1239:1239	P < .05	1237:1243	P < .05	1237:1243	RESULTS Compared with DSS group, ICA significantly improved disease activity (P < .05) and reduced inflammatory damage of colon tissues (P < .05) in DSS-induced colitis mice.
34231485	3	20	dep	METHODS	369:375	arg1	divided					419:425	divided	419:425	were randomly divided into the Control group, dextran sodium sulfate (DSS)-induced colitis (DSS) group, and ICA treatment (DSS+ICA) group	405:541	METHODS Fifteen female C57BL/6 mice were randomly divided into the Control group, dextran sodium sulfate (DSS)-induced colitis (DSS) group, and ICA treatment (DSS+ICA) group.
34231485	14	21	theme	flora	1869:1873	arg1	abundance					1875:1883	intestinal flora abundance	1858:1883	intestinal flora abundance	1858:1883	CONCLUSION ICA can improve intestinal flora abundance and composition of DSS-induced colitis mice, and inhibit tissue damage and inflammatory response through modulating the p-p65/p65 expression.
34231485	0	22	theme	Modulating	119:128	arg1	Molecule					140:147	Modulating p-p65/p65 Molecule	119:147	Modulating p-p65/p65 Molecule	119:147	Icariin Inhibits Intestinal Inflammation of DSS-Induced Colitis Mice Through Modulating Intestinal Flora Abundance and Modulating p-p65/p65 Molecule.
34231485	8	23	theme	rDNA	1008:1011	arg1	sequencing					1013:1022	16S rDNA sequencing	1004:1022	16S rDNA sequencing	1004:1022	16S rDNA sequencing was used to analyze the abundance and composition of intestinal microflora.
34231485	10	24	theme	P	1376:1376	arg1	length					1368:1373	colon length	1362:1373	colon length (P < .05)	1362:1383	Compared with the DSS group, mice in the ICA group demonstrated significant weight and colon length (P < .05).
34231485	10	24	theme	P	1376:1376	arg1	<					1378:1378	P < .05	1376:1382	P < .05	1376:1382	Compared with the DSS group, mice in the ICA group demonstrated significant weight and colon length (P < .05).
34231485	13	25	theme	beneficial	1721:1730	arg1	microflora					1732:1741	beneficial microflora	1721:1741	beneficial microflora (Lactobacillus, Lachnospiraceae, Akkermansia)	1721:1787	ICA significantly reduced the proportion and activity of Bacteroides, Helicobacteraceae, Turicibacter, and significantly increased that of beneficial microflora (Lactobacillus, Lachnospiraceae, Akkermansia), so as improved damages of colon tissues.
34231485	13	25	theme	beneficial	1721:1730	arg1	Akkermansia					1776:1786	Akkermansia	1776:1786	Akkermansia	1776:1786	ICA significantly reduced the proportion and activity of Bacteroides, Helicobacteraceae, Turicibacter, and significantly increased that of beneficial microflora (Lactobacillus, Lachnospiraceae, Akkermansia), so as improved damages of colon tissues.
34231485	6	26	link	enzyme-linked	882:894	arg1	assay					910:914	enzyme-linked immunosorbent assay	882:914	enzyme-linked immunosorbent assay	882:914	Serum levels of TNF-α and IL-6 were detected with enzyme-linked immunosorbent assay.
34231485	9	27	theme	colitis	1261:1267	arg1	mice					1269:1272	DSS-induced colitis mice	1249:1272	DSS-induced colitis mice	1249:1272	RESULTS Compared with DSS group, ICA significantly improved disease activity (P < .05) and reduced inflammatory damage of colon tissues (P < .05) in DSS-induced colitis mice.
34231485	11	28	theme	TNF-α	1438:1442	arg1	expressions					1414:1424	expressions	1414:1424	expressions of IL-6 and TNF-α	1414:1442	ICA significantly inhibited expressions of IL-6 and TNF-α compared to the DSS group (P < .05).
34231485	4	29	theme	stool-bleeding	707:720	arg1	scoring					722:728	stool-bleeding scoring	707:728	stool-bleeding scoring	707:728	The severity of inflammation in DSS-induced colitis mice was evaluated using disease activity scoring (considering weight-loss percentage, stool-shape change, and stool-bleeding scoring).
34231485	0	30	theme	Intestinal	17:26	arg1	Inflammation					28:39	Intestinal Inflammation	17:39	Intestinal Inflammation of DSS-Induced Colitis Mice	17:67	Icariin Inhibits Intestinal Inflammation of DSS-Induced Colitis Mice Through Modulating Intestinal Flora Abundance and Modulating p-p65/p65 Molecule.
34231485	14	31	theme	mice	1924:1927	arg1	composition					1889:1899	composition	1889:1899	composition	1889:1899	CONCLUSION ICA can improve intestinal flora abundance and composition of DSS-induced colitis mice, and inhibit tissue damage and inflammatory response through modulating the p-p65/p65 expression.
34231485	14	31	theme	mice	1924:1927	arg1	abundance					1875:1883	intestinal flora abundance	1858:1883	intestinal flora abundance	1858:1883	CONCLUSION ICA can improve intestinal flora abundance and composition of DSS-induced colitis mice, and inhibit tissue damage and inflammatory response through modulating the p-p65/p65 expression.
34231485	3	32	theme	Control	436:442	arg1	group					444:448	the Control group	432:448	the Control group	432:448	METHODS Fifteen female C57BL/6 mice were randomly divided into the Control group, dextran sodium sulfate (DSS)-induced colitis (DSS) group, and ICA treatment (DSS+ICA) group.
34231485	3	33	theme	female	385:390	arg1	mice					400:403	Fifteen female C57BL/6 mice	377:403	Fifteen female C57BL/6 mice	377:403	METHODS Fifteen female C57BL/6 mice were randomly divided into the Control group, dextran sodium sulfate (DSS)-induced colitis (DSS) group, and ICA treatment (DSS+ICA) group.
34231485	14	34	theme	DSS-induced	1904:1914	arg1	colitis					1916:1922	DSS-induced colitis	1904:1922	DSS-induced colitis mice	1904:1927	CONCLUSION ICA can improve intestinal flora abundance and composition of DSS-induced colitis mice, and inhibit tissue damage and inflammatory response through modulating the p-p65/p65 expression.
34231485	0	35	theme	Colitis	56:62	arg1	Mice					64:67	DSS-Induced Colitis Mice	44:67	DSS-Induced Colitis Mice	44:67	Icariin Inhibits Intestinal Inflammation of DSS-Induced Colitis Mice Through Modulating Intestinal Flora Abundance and Modulating p-p65/p65 Molecule.
34231485	13	36	theme	tissues	1822:1828	arg1	damages					1805:1811	damages	1805:1811	damages of colon tissues	1805:1828	ICA significantly reduced the proportion and activity of Bacteroides, Helicobacteraceae, Turicibacter, and significantly increased that of beneficial microflora (Lactobacillus, Lachnospiraceae, Akkermansia), so as improved damages of colon tissues.
34231485	10	37	theme	DSS	1293:1295	arg1	group					1297:1301	the DSS group	1289:1301	the DSS group	1289:1301	Compared with the DSS group, mice in the ICA group demonstrated significant weight and colon length (P < .05).
34231485	2	38	theme	colitis	355:361	arg1	mice					363:366	colitis mice	355:366	colitis mice	355:366	This study aimed to investigate the effect of icariin (ICA) on the intestinal microflora of colitis mice.
34231485	0	39	theme	Mice	64:67	arg1	Inflammation					28:39	Intestinal Inflammation	17:39	Intestinal Inflammation of DSS-Induced Colitis Mice	17:67	Icariin Inhibits Intestinal Inflammation of DSS-Induced Colitis Mice Through Modulating Intestinal Flora Abundance and Modulating p-p65/p65 Molecule.
34231485	6	40	theme	enzyme-linked	882:894	arg1	assay					910:914	enzyme-linked immunosorbent assay	882:914	enzyme-linked immunosorbent assay	882:914	Serum levels of TNF-α and IL-6 were detected with enzyme-linked immunosorbent assay.
34231485	4	41	from	severity	548:555	arg1	mice					596:599	DSS-induced colitis mice	576:599	DSS-induced colitis mice	576:599	The severity of inflammation in DSS-induced colitis mice was evaluated using disease activity scoring (considering weight-loss percentage, stool-shape change, and stool-bleeding scoring).
34231485	9	42	from	damage	1212:1217	arg1	mice					1269:1272	DSS-induced colitis mice	1249:1272	DSS-induced colitis mice	1249:1272	RESULTS Compared with DSS group, ICA significantly improved disease activity (P < .05) and reduced inflammatory damage of colon tissues (P < .05) in DSS-induced colitis mice.
34231485	6	43	theme	IL-6	858:861	arg1	levels					838:843	Serum levels	832:843	Serum levels of TNF-α and IL-6	832:861	Serum levels of TNF-α and IL-6 were detected with enzyme-linked immunosorbent assay.
34231485	9	44	theme	disease	1160:1166	arg1	activity					1168:1175	disease activity	1160:1175	disease activity (P < .05)	1160:1185	RESULTS Compared with DSS group, ICA significantly improved disease activity (P < .05) and reduced inflammatory damage of colon tissues (P < .05) in DSS-induced colitis mice.
34231485	9	44	theme	disease	1160:1166	arg1	<					1180:1180	P < .05	1178:1184	P < .05	1178:1184	RESULTS Compared with DSS group, ICA significantly improved disease activity (P < .05) and reduced inflammatory damage of colon tissues (P < .05) in DSS-induced colitis mice.
34231485	5	45	theme	intestinal	761:770	arg1	tract					772:776	mice intestinal tract	756:776	mice intestinal tract	756:776	Pathological changes of mice intestinal tract were evaluated using hematoxylin-eosin (HE) staining.
34231485	6	46	theme	TNF-α	848:852	arg1	levels					838:843	Serum levels	832:843	Serum levels of TNF-α and IL-6	832:861	Serum levels of TNF-α and IL-6 were detected with enzyme-linked immunosorbent assay.
34231485	5	47	theme	Pathological	732:743	arg1	changes					745:751	Pathological changes	732:751	Pathological changes of mice intestinal tract	732:776	Pathological changes of mice intestinal tract were evaluated using hematoxylin-eosin (HE) staining.
34231485	3	48	theme	treatment	517:525	arg1	group					537:541	ICA treatment (DSS+ICA) group	513:541	ICA treatment (DSS+ICA) group	513:541	METHODS Fifteen female C57BL/6 mice were randomly divided into the Control group, dextran sodium sulfate (DSS)-induced colitis (DSS) group, and ICA treatment (DSS+ICA) group.
34231485	1	49	theme	inflammatory	194:205	arg1	IBD					222:224	IBD	222:224	IBD	222:224	BACKGROUND Ulcerative colitis, as a kind of inflammatory bowel disease (IBD) is characterized by abdominal pain.
34231485	1	49	theme	inflammatory	194:205	arg1	disease					213:219	inflammatory bowel disease	194:219	inflammatory bowel disease (IBD)	194:225	BACKGROUND Ulcerative colitis, as a kind of inflammatory bowel disease (IBD) is characterized by abdominal pain.
34231485	12	50	theme	p-p65/	1481:1486	arg1	ratio					1492:1496	p-p65/ p65 ratio	1481:1496	p-p65/ p65 ratio in the DSS + ICA group	1481:1519	p-p65/ p65 ratio in the DSS + ICA group was remarkably enhanced compared to the DSS group (P < .05).
34231485	10	51	theme	ICA	1316:1318	arg1	group					1320:1324	the ICA group	1312:1324	the ICA group	1312:1324	Compared with the DSS group, mice in the ICA group demonstrated significant weight and colon length (P < .05).
34231485	5	52	theme	hematoxylin-eosin	799:815	arg1	staining					822:829	hematoxylin-eosin (HE) staining	799:829	hematoxylin-eosin (HE) staining	799:829	Pathological changes of mice intestinal tract were evaluated using hematoxylin-eosin (HE) staining.
34231485	8	53	dep	abundance	1048:1056	arg1	the					1044:1046	the	1044:1046	the	1044:1046	16S rDNA sequencing was used to analyze the abundance and composition of intestinal microflora.
34231485	5	54	theme	HE	818:819	arg1	staining					822:829	hematoxylin-eosin (HE) staining	799:829	hematoxylin-eosin (HE) staining	799:829	Pathological changes of mice intestinal tract were evaluated using hematoxylin-eosin (HE) staining.
34231485	4	55	theme	colitis	588:594	arg1	mice					596:599	DSS-induced colitis mice	576:599	DSS-induced colitis mice	576:599	The severity of inflammation in DSS-induced colitis mice was evaluated using disease activity scoring (considering weight-loss percentage, stool-shape change, and stool-bleeding scoring).
34231485	14	56	theme	p-p65/p65	2005:2013	arg1	expression					2015:2024	the p-p65/p65 expression	2001:2024	the p-p65/p65 expression	2001:2024	CONCLUSION ICA can improve intestinal flora abundance and composition of DSS-induced colitis mice, and inhibit tissue damage and inflammatory response through modulating the p-p65/p65 expression.
34231485	7	57	theme	Western	984:990	arg1	assay					997:1001	Western blot assay	984:1001	Western blot assay	984:1001	Expressions of p65 and p-p65 (p-p65/p65 ratio) were analyzed using Western blot assay.
34231485	4	58	theme	weight-loss	659:669	arg1	percentage					671:680	weight-loss percentage	659:680	weight-loss percentage	659:680	The severity of inflammation in DSS-induced colitis mice was evaluated using disease activity scoring (considering weight-loss percentage, stool-shape change, and stool-bleeding scoring).
34231485	11	59	theme	DSS	1460:1462	arg1	group					1464:1468	the DSS group	1456:1468	the DSS group (P < .05)	1456:1478	ICA significantly inhibited expressions of IL-6 and TNF-α compared to the DSS group (P < .05).
34231485	11	59	theme	DSS	1460:1462	arg1	<					1473:1473	P < .05	1471:1477	P < .05	1471:1477	ICA significantly inhibited expressions of IL-6 and TNF-α compared to the DSS group (P < .05).
34231485	7	60	theme	blot	992:995	arg1	assay					997:1001	Western blot assay	984:1001	Western blot assay	984:1001	Expressions of p65 and p-p65 (p-p65/p65 ratio) were analyzed using Western blot assay.
34231485	1	61	theme	BACKGROUND	150:159	arg1	colitis					172:178	BACKGROUND Ulcerative colitis	150:178	BACKGROUND Ulcerative colitis	150:178	BACKGROUND Ulcerative colitis, as a kind of inflammatory bowel disease (IBD) is characterized by abdominal pain.
34231485	12	62	theme	DSS	1561:1563	arg1	group					1565:1569	the DSS group	1557:1569	the DSS group (P < .05)	1557:1579	p-p65/ p65 ratio in the DSS + ICA group was remarkably enhanced compared to the DSS group (P < .05).
34231485	12	62	theme	DSS	1561:1563	arg1	<					1574:1574	P < .05	1572:1578	P < .05	1572:1578	p-p65/ p65 ratio in the DSS + ICA group was remarkably enhanced compared to the DSS group (P < .05).
34231485	9	63	theme	colon	1222:1226	arg1	tissues					1228:1234	colon tissues	1222:1234	colon tissues (P < .05)	1222:1244	RESULTS Compared with DSS group, ICA significantly improved disease activity (P < .05) and reduced inflammatory damage of colon tissues (P < .05) in DSS-induced colitis mice.
34231485	9	63	theme	colon	1222:1226	arg1	<					1239:1239	P < .05	1237:1243	P < .05	1237:1243	RESULTS Compared with DSS group, ICA significantly improved disease activity (P < .05) and reduced inflammatory damage of colon tissues (P < .05) in DSS-induced colitis mice.
34231485	1	64	theme	disease	213:219	arg1	kind					186:189	a kind	184:189	a kind of inflammatory bowel disease (IBD)	184:225	BACKGROUND Ulcerative colitis, as a kind of inflammatory bowel disease (IBD) is characterized by abdominal pain.
34231485	4	65	theme	activity	629:636	arg1	scoring					638:644	disease activity scoring	621:644	disease activity scoring (considering weight-loss percentage, stool-shape change, and stool-bleeding scoring)	621:729	The severity of inflammation in DSS-induced colitis mice was evaluated using disease activity scoring (considering weight-loss percentage, stool-shape change, and stool-bleeding scoring).
34231485	0	66	theme	Flora	99:103	arg1	Abundance					105:113	Intestinal Flora Abundance	88:113	Intestinal Flora Abundance	88:113	Icariin Inhibits Intestinal Inflammation of DSS-Induced Colitis Mice Through Modulating Intestinal Flora Abundance and Modulating p-p65/p65 Molecule.
34231485	2	67	from	effect	299:304	arg1	microflora					341:350	the intestinal microflora	326:350	the intestinal microflora of colitis mice	326:366	This study aimed to investigate the effect of icariin (ICA) on the intestinal microflora of colitis mice.
34231485	10	68	theme	colon	1362:1366	arg1	length					1368:1373	colon length	1362:1373	colon length (P < .05)	1362:1383	Compared with the DSS group, mice in the ICA group demonstrated significant weight and colon length (P < .05).
34231485	10	68	theme	colon	1362:1366	arg1	<					1378:1378	P < .05	1376:1382	P < .05	1376:1382	Compared with the DSS group, mice in the ICA group demonstrated significant weight and colon length (P < .05).
34231485	8	69	theme	16S	1004:1006	arg1	sequencing					1013:1022	16S rDNA sequencing	1004:1022	16S rDNA sequencing	1004:1022	16S rDNA sequencing was used to analyze the abundance and composition of intestinal microflora.
34231485	7	70	theme	p-p65	940:944	arg1	Expressions					917:927	Expressions	917:927	Expressions of p65 and p-p65 (p-p65/p65 ratio)	917:962	Expressions of p65 and p-p65 (p-p65/p65 ratio) were analyzed using Western blot assay.
34231485	8	71	used	used	1028:1031	arg2	sequencing					1013:1022	16S rDNA sequencing	1004:1022	16S rDNA sequencing	1004:1022	16S rDNA sequencing was used to analyze the abundance and composition of intestinal microflora.
34231485	14	72	theme	inflammatory	1960:1971	arg1	response					1973:1980	inflammatory response	1960:1980	inflammatory response	1960:1980	CONCLUSION ICA can improve intestinal flora abundance and composition of DSS-induced colitis mice, and inhibit tissue damage and inflammatory response through modulating the p-p65/p65 expression.
34231485	7	73	theme	p65	932:934	arg1	Expressions					917:927	Expressions	917:927	Expressions of p65 and p-p65 (p-p65/p65 ratio)	917:962	Expressions of p65 and p-p65 (p-p65/p65 ratio) were analyzed using Western blot assay.
34231485	14	74	theme	intestinal	1858:1867	arg1	abundance					1875:1883	intestinal flora abundance	1858:1883	intestinal flora abundance	1858:1883	CONCLUSION ICA can improve intestinal flora abundance and composition of DSS-induced colitis mice, and inhibit tissue damage and inflammatory response through modulating the p-p65/p65 expression.
34231485	12	75	theme	ICA	1511:1513	arg1	group					1515:1519	the DSS + ICA group	1501:1519	group	1515:1519	p-p65/ p65 ratio in the DSS + ICA group was remarkably enhanced compared to the DSS group (P < .05).
34231485	5	76	theme	tract	772:776	arg1	changes					745:751	Pathological changes	732:751	Pathological changes of mice intestinal tract	732:776	Pathological changes of mice intestinal tract were evaluated using hematoxylin-eosin (HE) staining.
34231485	9	77	theme	DSS-induced	1249:1259	arg1	colitis					1261:1267	DSS-induced colitis	1249:1267	DSS-induced colitis mice	1249:1272	RESULTS Compared with DSS group, ICA significantly improved disease activity (P < .05) and reduced inflammatory damage of colon tissues (P < .05) in DSS-induced colitis mice.
34231485	13	78	theme	Turicibacter	1671:1682	arg1	activity					1627:1634	activity	1627:1634	activity	1627:1634	ICA significantly reduced the proportion and activity of Bacteroides, Helicobacteraceae, Turicibacter, and significantly increased that of beneficial microflora (Lactobacillus, Lachnospiraceae, Akkermansia), so as improved damages of colon tissues.
34231485	13	78	theme	Turicibacter	1671:1682	arg1	proportion					1612:1621	the proportion	1608:1621	the proportion	1608:1621	ICA significantly reduced the proportion and activity of Bacteroides, Helicobacteraceae, Turicibacter, and significantly increased that of beneficial microflora (Lactobacillus, Lachnospiraceae, Akkermansia), so as improved damages of colon tissues.
34231485	2	79	theme	mice	363:366	arg1	microflora					341:350	the intestinal microflora	326:350	the intestinal microflora of colitis mice	326:366	This study aimed to investigate the effect of icariin (ICA) on the intestinal microflora of colitis mice.
34231485	12	80	from	ratio	1492:1496	arg1	group					1515:1519	the DSS + ICA group	1501:1519	group	1515:1519	p-p65/ p65 ratio in the DSS + ICA group was remarkably enhanced compared to the DSS group (P < .05).
34231485	14	81	theme	CONCLUSION	1831:1840	arg1	ICA					1842:1844	CONCLUSION ICA	1831:1844	CONCLUSION ICA	1831:1844	CONCLUSION ICA can improve intestinal flora abundance and composition of DSS-induced colitis mice, and inhibit tissue damage and inflammatory response through modulating the p-p65/p65 expression.
34231485	9	82	theme	DSS	1122:1124	arg1	group					1126:1130	DSS group	1122:1130	DSS group	1122:1130	RESULTS Compared with DSS group, ICA significantly improved disease activity (P < .05) and reduced inflammatory damage of colon tissues (P < .05) in DSS-induced colitis mice.
34231485	3	83	theme	colitis	488:494	arg1	group					502:506	dextran sodium sulfate (DSS)-induced colitis (DSS) group	451:506	dextran sodium sulfate (DSS)-induced colitis (DSS) group	451:506	METHODS Fifteen female C57BL/6 mice were randomly divided into the Control group, dextran sodium sulfate (DSS)-induced colitis (DSS) group, and ICA treatment (DSS+ICA) group.
34231485	0	84	theme	DSS-Induced	44:54	arg1	Colitis					56:62	DSS-Induced Colitis	44:62	DSS-Induced Colitis Mice	44:67	Icariin Inhibits Intestinal Inflammation of DSS-Induced Colitis Mice Through Modulating Intestinal Flora Abundance and Modulating p-p65/p65 Molecule.
34231485	6	85	theme	Serum	832:836	arg1	levels					838:843	Serum levels	832:843	Serum levels of TNF-α and IL-6	832:861	Serum levels of TNF-α and IL-6 were detected with enzyme-linked immunosorbent assay.
34231485	12	86	theme	DSS	1505:1507	arg1	group					1515:1519	the DSS + ICA group	1501:1519	group	1515:1519	p-p65/ p65 ratio in the DSS + ICA group was remarkably enhanced compared to the DSS group (P < .05).
34231485	14	87	theme	colitis	1916:1922	arg1	mice					1924:1927	DSS-induced colitis mice	1904:1927	DSS-induced colitis mice	1904:1927	CONCLUSION ICA can improve intestinal flora abundance and composition of DSS-induced colitis mice, and inhibit tissue damage and inflammatory response through modulating the p-p65/p65 expression.
34231485	5	88	theme	mice	756:759	arg1	tract					772:776	mice intestinal tract	756:776	mice intestinal tract	756:776	Pathological changes of mice intestinal tract were evaluated using hematoxylin-eosin (HE) staining.
34231485	8	89	theme	microflora	1088:1097	arg1	composition					1062:1072	composition	1062:1072	composition	1062:1072	16S rDNA sequencing was used to analyze the abundance and composition of intestinal microflora.
34231485	8	89	theme	microflora	1088:1097	arg1	abundance					1048:1056	abundance	1048:1056	abundance	1048:1056	16S rDNA sequencing was used to analyze the abundance and composition of intestinal microflora.
34231485	10	90	from	mice	1304:1307	arg1	group					1320:1324	the ICA group	1312:1324	the ICA group	1312:1324	Compared with the DSS group, mice in the ICA group demonstrated significant weight and colon length (P < .05).
34231485	3	91	theme	C57BL/6	392:398	arg1	mice					400:403	Fifteen female C57BL/6 mice	377:403	Fifteen female C57BL/6 mice	377:403	METHODS Fifteen female C57BL/6 mice were randomly divided into the Control group, dextran sodium sulfate (DSS)-induced colitis (DSS) group, and ICA treatment (DSS+ICA) group.
34231485	11	92	theme	P	1471:1471	arg1	group					1464:1468	the DSS group	1456:1468	the DSS group (P < .05)	1456:1478	ICA significantly inhibited expressions of IL-6 and TNF-α compared to the DSS group (P < .05).
34231485	11	92	theme	P	1471:1471	arg1	<					1473:1473	P < .05	1471:1477	P < .05	1471:1477	ICA significantly inhibited expressions of IL-6 and TNF-α compared to the DSS group (P < .05).
34231485	8	93	theme	intestinal	1077:1086	arg1	microflora					1088:1097	intestinal microflora	1077:1097	intestinal microflora	1077:1097	16S rDNA sequencing was used to analyze the abundance and composition of intestinal microflora.
34231485	3	94	theme	-induced	479:486	arg1	DSS					497:499	DSS	497:499	DSS	497:499	METHODS Fifteen female C57BL/6 mice were randomly divided into the Control group, dextran sodium sulfate (DSS)-induced colitis (DSS) group, and ICA treatment (DSS+ICA) group.
34231485	3	94	theme	-induced	479:486	arg1	colitis					488:494	dextran sodium sulfate (DSS)-induced colitis	451:494	dextran sodium sulfate (DSS)-induced colitis (DSS) group	451:506	METHODS Fifteen female C57BL/6 mice were randomly divided into the Control group, dextran sodium sulfate (DSS)-induced colitis (DSS) group, and ICA treatment (DSS+ICA) group.
34231485	4	95	theme	inflammation	560:571	arg1	severity					548:555	The severity	544:555	The severity of inflammation in DSS-induced colitis mice	544:599	The severity of inflammation in DSS-induced colitis mice was evaluated using disease activity scoring (considering weight-loss percentage, stool-shape change, and stool-bleeding scoring).
34231485	1	96	theme	abdominal	247:255	arg1	pain					257:260	abdominal pain	247:260	abdominal pain	247:260	BACKGROUND Ulcerative colitis, as a kind of inflammatory bowel disease (IBD) is characterized by abdominal pain.
31992619	6	0	theme	increased	870:878	arg1	susceptibility					889:902	increased colistin susceptibility	870:902	increased colistin susceptibility in a group D Salmonella veterinary isolate	870:945	Whole-genome sequencing also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc.
31992619	2	1	theme	O-antigen	311:319	arg1	epitope					321:327	the O-antigen epitope	307:327	the O-antigen epitope in group D Salmonella	307:349	Here, we show that the O-antigen epitope in group D Salmonella governs the levels of colistin susceptibility.
31992619	8	2	theme	intrinsic	1318:1326	arg1	resistance					1337:1346	This observed intrinsic colistin resistance	1304:1346	This observed intrinsic colistin resistance	1304:1346	This observed intrinsic colistin resistance is of concern since this last-resort drug might no longer be effective for treating severe human infections with the most common Salmonella serovar, Salmonella enterica serovar Enteritidis.
31992619	10	3	dep	group	1740:1744	arg1	due					1782:1784	due	1782:1784	due	1782:1784	Using whole-genome sequencing, we also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc.
31992619	4	4	theme	deoxymannose	600:611	arg1	presence					555:562	The presence	551:562	The presence of dideoxyhexose, abequose, and the deoxymannose, tyvelose,	551:622	The presence of dideoxyhexose, abequose, and the deoxymannose, tyvelose, differentiate the Salmonella group B and group D O antigens, respectively.
31992619	9	5	theme	susceptibility	1632:1645	arg1	levels					1613:1618	the levels	1609:1618	the levels of colistin susceptibility	1609:1645	Here, we show that the O-antigen epitope in group D Salmonella governs the levels of colistin susceptibility.
31992619	10	6	theme	D	1746:1746	arg1	isolate					1770:1776	D Salmonella veterinary isolate	1746:1776	D Salmonella veterinary isolate	1746:1776	Using whole-genome sequencing, we also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc.
31992619	11	7	dep	presence	1909:1916	arg1	the					1905:1907	the	1905:1907	the	1905:1907	In summary, we show that two different mechanisms that influence the presence and composition of O antigens affect colistin susceptibility in Salmonella enterica.
31992619	6	8	theme	veterinary	928:937	arg1	isolate					939:945	D Salmonella veterinary isolate	915:945	increased colistin susceptibility in a group D Salmonella veterinary isolate	870:945	Whole-genome sequencing also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc.
31992619	4	9	theme	abequose	582:589	arg1	presence					555:562	The presence	551:562	The presence of dideoxyhexose, abequose, and the deoxymannose, tyvelose,	551:622	The presence of dideoxyhexose, abequose, and the deoxymannose, tyvelose, differentiate the Salmonella group B and group D O antigens, respectively.
31992619	7	10	dep	presence	1075:1082	arg1	the					1071:1073	the	1071:1073	the	1071:1073	This study shows that two different mechanisms that influence the presence and composition of O antigens affect colistin susceptibility in Salmonella entericaIMPORTANCE Some serovars of Salmonella, namely, those belonging to group D, appear to show a degree of intrinsic resistance to colistin.
31992619	3	11	theme	rfbSE	461:465	arg1	genes					467:471	the rfbSE genes	457:471	the rfbSE genes from a group D Salmonella	457:497	Substitution of the rfbJ gene in a group B Salmonella with the rfbSE genes from a group D Salmonella conferred a decrease in susceptibility to colistin.
31992619	6	12	theme	D	915:915	arg1	isolate					939:945	D Salmonella veterinary isolate	915:945	increased colistin susceptibility in a group D Salmonella veterinary isolate	870:945	Whole-genome sequencing also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc.
31992619	6	13	dep	susceptibility	889:902	arg1	isolate					939:945	D Salmonella veterinary isolate	915:945	increased colistin susceptibility in a group D Salmonella veterinary isolate	870:945	Whole-genome sequencing also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc.
31992619	8	14	theme	observed	1309:1316	arg1	resistance					1337:1346	This observed intrinsic colistin resistance	1304:1346	This observed intrinsic colistin resistance	1304:1346	This observed intrinsic colistin resistance is of concern since this last-resort drug might no longer be effective for treating severe human infections with the most common Salmonella serovar, Salmonella enterica serovar Enteritidis.
31992619	4	15	theme	B	659:659	arg1	antigens					675:682	group B and group D O antigens	653:682	the Salmonella group B and group D O antigens	638:682	The presence of dideoxyhexose, abequose, and the deoxymannose, tyvelose, differentiate the Salmonella group B and group D O antigens, respectively.
31992619	3	16	with	Substitution	398:409	arg1	genes					467:471	the rfbSE genes	457:471	the rfbSE genes from a group D Salmonella	457:497	Substitution of the rfbJ gene in a group B Salmonella with the rfbSE genes from a group D Salmonella conferred a decrease in susceptibility to colistin.
31992619	10	17	theme	veterinary	1759:1768	arg1	isolate					1770:1776	D Salmonella veterinary isolate	1746:1776	D Salmonella veterinary isolate	1746:1776	Using whole-genome sequencing, we also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc.
31992619	4	18	dep	antigens	675:682	arg1	Salmonella					642:651	the Salmonella group B and group D O antigens	638:682	the Salmonella group B and group D O antigens	638:682	The presence of dideoxyhexose, abequose, and the deoxymannose, tyvelose, differentiate the Salmonella group B and group D O antigens, respectively.
31992619	4	19	theme	group	665:669	arg1	D					671:671	group D	665:671	group D	665:671	The presence of dideoxyhexose, abequose, and the deoxymannose, tyvelose, differentiate the Salmonella group B and group D O antigens, respectively.
31992619	11	20	theme	colistin	1955:1962	arg1	susceptibility					1964:1977	colistin susceptibility	1955:1977	colistin susceptibility	1955:1977	In summary, we show that two different mechanisms that influence the presence and composition of O antigens affect colistin susceptibility in Salmonella enterica.
31992619	8	21	theme	severe	1432:1437	arg1	infections					1445:1454	severe human infections	1432:1454	severe human infections with the most common Salmonella serovar	1432:1494	This observed intrinsic colistin resistance is of concern since this last-resort drug might no longer be effective for treating severe human infections with the most common Salmonella serovar, Salmonella enterica serovar Enteritidis.
31992619	8	21	theme	severe	1432:1437	arg1	Enteritidis					1525:1535	Salmonella enterica serovar Enteritidis	1497:1535	Salmonella enterica serovar Enteritidis	1497:1535	This observed intrinsic colistin resistance is of concern since this last-resort drug might no longer be effective for treating severe human infections with the most common Salmonella serovar, Salmonella enterica serovar Enteritidis.
31992619	11	22	theme	different	1869:1877	arg1	mechanisms					1879:1888	two different mechanisms	1865:1888	two different mechanisms that influence the presence and composition of O antigens	1865:1946	In summary, we show that two different mechanisms that influence the presence and composition of O antigens affect colistin susceptibility in Salmonella enterica.
31992619	1	23	dep	D	87:87	arg1	serovars					121:128	Salmonella enterica serovars	101:128	Salmonella enterica serovars	101:128	Group D and group B Salmonella enterica serovars differ in their susceptibility to colistin with the former frequently intrinsically resistant (MIC > 2 μg/ml); however, the mechanism has not been described.
31992619	3	24	from	group	480:484	arg1	genes					467:471	the rfbSE genes	457:471	the rfbSE genes from a group D Salmonella	457:497	Substitution of the rfbJ gene in a group B Salmonella with the rfbSE genes from a group D Salmonella conferred a decrease in susceptibility to colistin.
31992619	11	25	theme	O	1937:1937	arg1	antigens					1939:1946	O antigens	1937:1946	O antigens	1937:1946	In summary, we show that two different mechanisms that influence the presence and composition of O antigens affect colistin susceptibility in Salmonella enterica.
31992619	3	26	dep	B	439:439	arg1	Salmonella					441:450	a group B Salmonella	431:450	a group B Salmonella	431:450	Substitution of the rfbJ gene in a group B Salmonella with the rfbSE genes from a group D Salmonella conferred a decrease in susceptibility to colistin.
31992619	1	27	dep	Salmonella	101:110	arg1	enterica					112:119	enterica	112:119	enterica	112:119	Group D and group B Salmonella enterica serovars differ in their susceptibility to colistin with the former frequently intrinsically resistant (MIC > 2 μg/ml); however, the mechanism has not been described.
31992619	7	28	theme	Salmonella	1148:1157	arg1	entericaIMPORTANCE					1159:1176	Salmonella entericaIMPORTANCE	1148:1176	Salmonella entericaIMPORTANCE Some serovars of Salmonella, namely, those belonging to group D, appear to show a degree of intrinsic resistance to colistin	1148:1301	This study shows that two different mechanisms that influence the presence and composition of O antigens affect colistin susceptibility in Salmonella entericaIMPORTANCE Some serovars of Salmonella, namely, those belonging to group D, appear to show a degree of intrinsic resistance to colistin.
31992619	1	29	theme	Group	81:85	arg1	D					87:87	Group D	81:87	Group D	81:87	Group D and group B Salmonella enterica serovars differ in their susceptibility to colistin with the former frequently intrinsically resistant (MIC > 2 μg/ml); however, the mechanism has not been described.
31992619	9	30	from	epitope	1571:1577	arg1	D					1588:1588	group D Salmonella	1582:1599	group D Salmonella	1582:1599	Here, we show that the O-antigen epitope in group D Salmonella governs the levels of colistin susceptibility.
31992619	7	31	theme	resistance	1280:1289	arg1	degree					1260:1265	a degree	1258:1265	a degree of intrinsic resistance to colistin	1258:1301	This study shows that two different mechanisms that influence the presence and composition of O antigens affect colistin susceptibility in Salmonella entericaIMPORTANCE Some serovars of Salmonella, namely, those belonging to group D, appear to show a degree of intrinsic resistance to colistin.
31992619	4	32	theme	O	673:673	arg1	antigens					675:682	group B and group D O antigens	653:682	the Salmonella group B and group D O antigens	638:682	The presence of dideoxyhexose, abequose, and the deoxymannose, tyvelose, differentiate the Salmonella group B and group D O antigens, respectively.
31992619	2	33	theme	susceptibility	382:395	arg1	levels					363:368	the levels	359:368	the levels of colistin susceptibility	359:395	Here, we show that the O-antigen epitope in group D Salmonella governs the levels of colistin susceptibility.
31992619	11	34	theme	antigens	1939:1946	arg1	composition					1922:1932	composition	1922:1932	composition	1922:1932	In summary, we show that two different mechanisms that influence the presence and composition of O antigens affect colistin susceptibility in Salmonella enterica.
31992619	11	34	theme	antigens	1939:1946	arg1	presence					1909:1916	presence	1909:1916	presence	1909:1916	In summary, we show that two different mechanisms that influence the presence and composition of O antigens affect colistin susceptibility in Salmonella enterica.
31992619	10	35	theme	polymerase	1815:1824	arg1	Rfc					1835:1837	Rfc	1835:1837	Rfc	1835:1837	Using whole-genome sequencing, we also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc.
31992619	10	35	theme	polymerase	1815:1824	arg1	protein					1826:1832	the O-antigen polymerase protein	1801:1832	the O-antigen polymerase protein	1801:1832	Using whole-genome sequencing, we also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc.
31992619	3	36	theme	rfbJ	418:421	arg1	gene					423:426	the rfbJ gene	414:426	the rfbJ gene	414:426	Substitution of the rfbJ gene in a group B Salmonella with the rfbSE genes from a group D Salmonella conferred a decrease in susceptibility to colistin.
31992619	7	37	theme	O	1103:1103	arg1	antigens					1105:1112	O antigens	1103:1112	O antigens	1103:1112	This study shows that two different mechanisms that influence the presence and composition of O antigens affect colistin susceptibility in Salmonella entericaIMPORTANCE Some serovars of Salmonella, namely, those belonging to group D, appear to show a degree of intrinsic resistance to colistin.
31992619	6	38	theme	polymerase	984:993	arg1	Rfc					1004:1006	Rfc	1004:1006	Rfc	1004:1006	Whole-genome sequencing also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc.
31992619	6	38	theme	polymerase	984:993	arg1	protein					995:1001	the O-antigen polymerase protein	970:1001	the O-antigen polymerase protein	970:1001	Whole-genome sequencing also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc.
31992619	9	39	theme	O-antigen	1561:1569	arg1	epitope					1571:1577	the O-antigen epitope	1557:1577	the O-antigen epitope in group D Salmonella	1557:1599	Here, we show that the O-antigen epitope in group D Salmonella governs the levels of colistin susceptibility.
31992619	7	40	dep	entericaIMPORTANCE	1159:1176	arg1	appear					1243:1248	appear	1243:1248	appear to show a degree of intrinsic resistance to colistin	1243:1301	This study shows that two different mechanisms that influence the presence and composition of O antigens affect colistin susceptibility in Salmonella entericaIMPORTANCE Some serovars of Salmonella, namely, those belonging to group D, appear to show a degree of intrinsic resistance to colistin.
31992619	8	41	theme	last-resort	1373:1383	arg1	drug					1385:1388	this last-resort drug	1368:1388	this last-resort drug	1368:1388	This observed intrinsic colistin resistance is of concern since this last-resort drug might no longer be effective for treating severe human infections with the most common Salmonella serovar, Salmonella enterica serovar Enteritidis.
31992619	10	42	theme	whole-genome	1654:1665	arg1	sequencing					1667:1676	whole-genome sequencing	1654:1676	whole-genome sequencing	1654:1676	Using whole-genome sequencing, we also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc.
31992619	1	43	theme	MIC	225:227	arg1	2 μg/ml					231:237	MIC > 2 μg/ml	225:237	MIC > 2 μg/ml	225:237	Group D and group B Salmonella enterica serovars differ in their susceptibility to colistin with the former frequently intrinsically resistant (MIC > 2 μg/ml); however, the mechanism has not been described.
31992619	7	44	theme	group	1234:1238	arg1	D					1240:1240	group D	1234:1240	group D	1234:1240	This study shows that two different mechanisms that influence the presence and composition of O antigens affect colistin susceptibility in Salmonella entericaIMPORTANCE Some serovars of Salmonella, namely, those belonging to group D, appear to show a degree of intrinsic resistance to colistin.
31992619	3	45	from	Substitution	398:409	arg1	B					439:439	a group B Salmonella	431:450	a group B Salmonella	431:450	Substitution of the rfbJ gene in a group B Salmonella with the rfbSE genes from a group D Salmonella conferred a decrease in susceptibility to colistin.
31992619	2	46	theme	group	332:336	arg1	D					338:338	group D Salmonella	332:349	group D Salmonella	332:349	Here, we show that the O-antigen epitope in group D Salmonella governs the levels of colistin susceptibility.
31992619	6	47	theme	colistin	880:887	arg1	susceptibility					889:902	increased colistin susceptibility	870:902	increased colistin susceptibility in a group D Salmonella veterinary isolate	870:945	Whole-genome sequencing also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc.
31992619	3	48	theme	D	486:486	arg1	group					480:484	a group D Salmonella	478:497	a group D Salmonella	478:497	Substitution of the rfbJ gene in a group B Salmonella with the rfbSE genes from a group D Salmonella conferred a decrease in susceptibility to colistin.
31992619	7	49	theme	different	1035:1043	arg1	mechanisms					1045:1054	two different mechanisms	1031:1054	two different mechanisms that influence the presence and composition of O antigens	1031:1112	This study shows that two different mechanisms that influence the presence and composition of O antigens affect colistin susceptibility in Salmonella entericaIMPORTANCE Some serovars of Salmonella, namely, those belonging to group D, appear to show a degree of intrinsic resistance to colistin.
31992619	10	50	theme	colistin	1711:1718	arg1	susceptibility					1720:1733	colistin susceptibility	1711:1733	colistin susceptibility	1711:1733	Using whole-genome sequencing, we also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc.
31992619	6	51	theme	Whole-genome	827:838	arg1	sequencing					840:849	Whole-genome sequencing	827:849	Whole-genome sequencing	827:849	Whole-genome sequencing also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc.
31992619	9	52	theme	colistin	1623:1630	arg1	susceptibility					1632:1645	colistin susceptibility	1623:1645	colistin susceptibility	1623:1645	Here, we show that the O-antigen epitope in group D Salmonella governs the levels of colistin susceptibility.
31992619	2	53	from	epitope	321:327	arg1	D					338:338	group D Salmonella	332:349	group D Salmonella	332:349	Here, we show that the O-antigen epitope in group D Salmonella governs the levels of colistin susceptibility.
31992619	3	54	dep	group	480:484	arg1	Salmonella					488:497	a group D Salmonella	478:497	a group D Salmonella	478:497	Substitution of the rfbJ gene in a group B Salmonella with the rfbSE genes from a group D Salmonella conferred a decrease in susceptibility to colistin.
31992619	10	55	theme	Salmonella	1748:1757	arg1	isolate					1770:1776	D Salmonella veterinary isolate	1746:1776	D Salmonella veterinary isolate	1746:1776	Using whole-genome sequencing, we also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc.
31992619	6	56	theme	Salmonella	917:926	arg1	isolate					939:945	D Salmonella veterinary isolate	915:945	increased colistin susceptibility in a group D Salmonella veterinary isolate	870:945	Whole-genome sequencing also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc.
31992619	9	57	dep	D	1588:1588	arg1	Salmonella					1590:1599	group D Salmonella	1582:1599	group D Salmonella	1582:1599	Here, we show that the O-antigen epitope in group D Salmonella governs the levels of colistin susceptibility.
31992619	5	58	theme	colistin	783:790	arg1	molecule					792:799	the colistin molecule	779:799	the colistin molecule	779:799	We hypothesize that the subtle difference between abequose and tyvelose hinders the colistin molecule from reaching its target.
31992619	1	59	theme	Salmonella	101:110	arg1	serovars					121:128	Salmonella enterica serovars	101:128	Salmonella enterica serovars	101:128	Group D and group B Salmonella enterica serovars differ in their susceptibility to colistin with the former frequently intrinsically resistant (MIC > 2 μg/ml); however, the mechanism has not been described.
31992619	4	60	theme	group	653:657	arg1	B					659:659	group B	653:659	group B	653:659	The presence of dideoxyhexose, abequose, and the deoxymannose, tyvelose, differentiate the Salmonella group B and group D O antigens, respectively.
31992619	1	61	dep	resistant	214:222	arg1	2 μg/ml					231:237	MIC > 2 μg/ml	225:237	MIC > 2 μg/ml	225:237	Group D and group B Salmonella enterica serovars differ in their susceptibility to colistin with the former frequently intrinsically resistant (MIC > 2 μg/ml); however, the mechanism has not been described.
31992619	6	62	theme	O-antigen	974:982	arg1	Rfc					1004:1006	Rfc	1004:1006	Rfc	1004:1006	Whole-genome sequencing also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc.
31992619	6	62	theme	O-antigen	974:982	arg1	protein					995:1001	the O-antigen polymerase protein	970:1001	the O-antigen polymerase protein	970:1001	Whole-genome sequencing also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc.
31992619	8	63	theme	human	1439:1443	arg1	infections					1445:1454	severe human infections	1432:1454	severe human infections with the most common Salmonella serovar	1432:1494	This observed intrinsic colistin resistance is of concern since this last-resort drug might no longer be effective for treating severe human infections with the most common Salmonella serovar, Salmonella enterica serovar Enteritidis.
31992619	8	63	theme	human	1439:1443	arg1	Enteritidis					1525:1535	Salmonella enterica serovar Enteritidis	1497:1535	Salmonella enterica serovar Enteritidis	1497:1535	This observed intrinsic colistin resistance is of concern since this last-resort drug might no longer be effective for treating severe human infections with the most common Salmonella serovar, Salmonella enterica serovar Enteritidis.
31992619	3	64	from	decrease	511:518	arg1	susceptibility					523:536	susceptibility	523:536	susceptibility to colistin	523:548	Substitution of the rfbJ gene in a group B Salmonella with the rfbSE genes from a group D Salmonella conferred a decrease in susceptibility to colistin.
31992619	3	65	theme	group	433:437	arg1	B					439:439	a group B Salmonella	431:450	a group B Salmonella	431:450	Substitution of the rfbJ gene in a group B Salmonella with the rfbSE genes from a group D Salmonella conferred a decrease in susceptibility to colistin.
31992619	7	66	theme	Salmonella	1195:1204	arg1	those					1215:1219	those	1215:1219	those	1215:1219	This study shows that two different mechanisms that influence the presence and composition of O antigens affect colistin susceptibility in Salmonella entericaIMPORTANCE Some serovars of Salmonella, namely, those belonging to group D, appear to show a degree of intrinsic resistance to colistin.
31992619	7	66	theme	Salmonella	1195:1204	arg1	serovars					1183:1190	Some serovars	1178:1190	Some serovars of Salmonella	1178:1204	This study shows that two different mechanisms that influence the presence and composition of O antigens affect colistin susceptibility in Salmonella entericaIMPORTANCE Some serovars of Salmonella, namely, those belonging to group D, appear to show a degree of intrinsic resistance to colistin.
31992619	6	67	from	defect	960:965	arg1	Rfc					1004:1006	Rfc	1004:1006	Rfc	1004:1006	Whole-genome sequencing also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc.
31992619	6	67	from	defect	960:965	arg1	protein					995:1001	the O-antigen polymerase protein	970:1001	the O-antigen polymerase protein	970:1001	Whole-genome sequencing also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc.
31992619	7	68	theme	intrinsic	1270:1278	arg1	resistance					1280:1289	intrinsic resistance	1270:1289	intrinsic resistance to colistin	1270:1301	This study shows that two different mechanisms that influence the presence and composition of O antigens affect colistin susceptibility in Salmonella entericaIMPORTANCE Some serovars of Salmonella, namely, those belonging to group D, appear to show a degree of intrinsic resistance to colistin.
31992619	0	69	theme	O-Antigen	4:12	arg1	Epitope					14:20	The O-Antigen Epitope	0:20	The O-Antigen Epitope	0:20	The O-Antigen Epitope Governs Susceptibility to Colistin in Salmonella enterica.
31992619	4	70	theme	D	671:671	arg1	antigens					675:682	group B and group D O antigens	653:682	the Salmonella group B and group D O antigens	638:682	The presence of dideoxyhexose, abequose, and the deoxymannose, tyvelose, differentiate the Salmonella group B and group D O antigens, respectively.
31992619	8	71	with	infections	1445:1454	arg1	serovar					1488:1494	the most common Salmonella serovar	1461:1494	the most common Salmonella serovar	1461:1494	This observed intrinsic colistin resistance is of concern since this last-resort drug might no longer be effective for treating severe human infections with the most common Salmonella serovar, Salmonella enterica serovar Enteritidis.
31992619	7	72	theme	antigens	1105:1112	arg1	composition					1088:1098	composition	1088:1098	composition	1088:1098	This study shows that two different mechanisms that influence the presence and composition of O antigens affect colistin susceptibility in Salmonella entericaIMPORTANCE Some serovars of Salmonella, namely, those belonging to group D, appear to show a degree of intrinsic resistance to colistin.
31992619	7	72	theme	antigens	1105:1112	arg1	presence					1075:1082	presence	1075:1082	presence	1075:1082	This study shows that two different mechanisms that influence the presence and composition of O antigens affect colistin susceptibility in Salmonella entericaIMPORTANCE Some serovars of Salmonella, namely, those belonging to group D, appear to show a degree of intrinsic resistance to colistin.
31992619	1	73	theme	group	93:97	arg1	B					99:99	group B	93:99	group B	93:99	Group D and group B Salmonella enterica serovars differ in their susceptibility to colistin with the former frequently intrinsically resistant (MIC > 2 μg/ml); however, the mechanism has not been described.
31992619	8	74	theme	colistin	1328:1335	arg1	resistance					1337:1346	This observed intrinsic colistin resistance	1304:1346	This observed intrinsic colistin resistance	1304:1346	This observed intrinsic colistin resistance is of concern since this last-resort drug might no longer be effective for treating severe human infections with the most common Salmonella serovar, Salmonella enterica serovar Enteritidis.
31992619	2	75	theme	colistin	373:380	arg1	susceptibility					382:395	colistin susceptibility	373:395	colistin susceptibility	373:395	Here, we show that the O-antigen epitope in group D Salmonella governs the levels of colistin susceptibility.
31992619	10	76	from	defect	1791:1796	arg1	Rfc					1835:1837	Rfc	1835:1837	Rfc	1835:1837	Using whole-genome sequencing, we also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc.
31992619	10	76	from	defect	1791:1796	arg1	protein					1826:1832	the O-antigen polymerase protein	1801:1832	the O-antigen polymerase protein	1801:1832	Using whole-genome sequencing, we also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc.
31992619	10	77	theme	O-antigen	1805:1813	arg1	Rfc					1835:1837	Rfc	1835:1837	Rfc	1835:1837	Using whole-genome sequencing, we also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc.
31992619	10	77	theme	O-antigen	1805:1813	arg1	protein					1826:1832	the O-antigen polymerase protein	1801:1832	the O-antigen polymerase protein	1801:1832	Using whole-genome sequencing, we also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc.
31992619	3	78	theme	gene	423:426	arg1	Substitution					398:409	Substitution	398:409	Substitution of the rfbJ gene in a group B Salmonella with the rfbSE genes from a group D Salmonella	398:497	Substitution of the rfbJ gene in a group B Salmonella with the rfbSE genes from a group D Salmonella conferred a decrease in susceptibility to colistin.
31992619	5	79	theme	subtle	723:728	arg1	difference					730:739	the subtle difference	719:739	the subtle difference between abequose and tyvelose	719:769	We hypothesize that the subtle difference between abequose and tyvelose hinders the colistin molecule from reaching its target.
31992619	6	80	from	susceptibility	889:902	arg1	group					909:913	a group	907:913	a group	907:913	Whole-genome sequencing also revealed that increased colistin susceptibility in a group D Salmonella veterinary isolate was due to a defect in the O-antigen polymerase protein, Rfc.
31992619	2	81	dep	D	338:338	arg1	Salmonella					340:349	group D Salmonella	332:349	group D Salmonella	332:349	Here, we show that the O-antigen epitope in group D Salmonella governs the levels of colistin susceptibility.
31992619	1	82	theme	>	229:229	arg1	2 μg/ml					231:237	MIC > 2 μg/ml	225:237	MIC > 2 μg/ml	225:237	Group D and group B Salmonella enterica serovars differ in their susceptibility to colistin with the former frequently intrinsically resistant (MIC > 2 μg/ml); however, the mechanism has not been described.
31992619	9	83	theme	group	1582:1586	arg1	D					1588:1588	group D Salmonella	1582:1599	group D Salmonella	1582:1599	Here, we show that the O-antigen epitope in group D Salmonella governs the levels of colistin susceptibility.
31992619	4	84	theme	dideoxyhexose	567:579	arg1	presence					555:562	The presence	551:562	The presence of dideoxyhexose, abequose, and the deoxymannose, tyvelose,	551:622	The presence of dideoxyhexose, abequose, and the deoxymannose, tyvelose, differentiate the Salmonella group B and group D O antigens, respectively.
31992619	7	85	theme	colistin	1121:1128	arg1	susceptibility					1130:1143	colistin susceptibility	1121:1143	colistin susceptibility	1121:1143	This study shows that two different mechanisms that influence the presence and composition of O antigens affect colistin susceptibility in Salmonella entericaIMPORTANCE Some serovars of Salmonella, namely, those belonging to group D, appear to show a degree of intrinsic resistance to colistin.
32235344	0	0	theme	Hyaluronic	100:109	arg1	Acid					111:114	Hyaluronic Acid	100:114	Hyaluronic Acid	100:114	Design of Experiments-Assisted Development of Clotrimazole-Loaded Ionic Polymeric Micelles Based on Hyaluronic Acid.
32235344	5	1	from	temperature	947:957	arg1	%					928:928	10% to 30%	919:928	10% to 30%	919:928	A 23 full factorial experimental design was used to evaluate the effect of the following factors: HA/HDA ratio from 1:0.25 to 1:0.75, cholesterol (CHOL%) as percentage of HA from 10% to 30%, and preparation temperature from 20 to 40 °C.
32235344	1	2	theme	core-shell	274:283	arg1	structure					286:294	their characteristic "core-shell" structure	252:294	their characteristic "core-shell" structure	252:294	Polymeric micelles based on amphiphilic polysaccharides have some advantages as a carrier of poorly soluble lipophilic drugs thanks to their characteristic "core-shell" structure.
32235344	5	3	theme	experimental	760:771	arg1	design					773:778	A 23 full factorial experimental design	740:778	A 23 full factorial experimental design	740:778	A 23 full factorial experimental design was used to evaluate the effect of the following factors: HA/HDA ratio from 1:0.25 to 1:0.75, cholesterol (CHOL%) as percentage of HA from 10% to 30%, and preparation temperature from 20 to 40 °C.
32235344	5	4	from	percentage	897:906	arg1	%					928:928	10% to 30%	919:928	10% to 30%	919:928	A 23 full factorial experimental design was used to evaluate the effect of the following factors: HA/HDA ratio from 1:0.25 to 1:0.75, cholesterol (CHOL%) as percentage of HA from 10% to 30%, and preparation temperature from 20 to 40 °C.
32235344	1	5	theme	"	284:284	arg1	structure					286:294	their characteristic "core-shell" structure	252:294	their characteristic "core-shell" structure	252:294	Polymeric micelles based on amphiphilic polysaccharides have some advantages as a carrier of poorly soluble lipophilic drugs thanks to their characteristic "core-shell" structure.
32235344	8	6	theme	formulation	1322:1332	arg1	effects					1248:1254	The effects	1244:1254	The effects of the formulation variables	1244:1283	The effects of the formulation variables and the composition of the optimized formulation were confirmed by a mixture design.
32235344	8	6	theme	formulation	1322:1332	arg1	composition					1293:1303	the composition	1289:1303	the composition of the optimized formulation	1289:1332	The effects of the formulation variables and the composition of the optimized formulation were confirmed by a mixture design.
32235344	2	7	theme	polymeric	315:323	arg1	micelles					325:332	ionic polymeric micelles	309:332	ionic polymeric micelles based on chitosan and fatty acids	309:366	Previously, ionic polymeric micelles based on chitosan and fatty acids have been developed.
32235344	8	8	theme	variables	1275:1283	arg1	effects					1248:1254	The effects	1244:1254	The effects of the formulation variables	1244:1283	The effects of the formulation variables and the composition of the optimized formulation were confirmed by a mixture design.
32235344	8	8	theme	variables	1275:1283	arg1	composition					1293:1303	the composition	1289:1303	the composition of the optimized formulation	1289:1332	The effects of the formulation variables and the composition of the optimized formulation were confirmed by a mixture design.
32235344	3	9	theme	study	412:416	arg1	aim					393:395	The aim	389:395	The aim of the present study	389:416	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	9	theme	study	412:416	arg1	preparation					426:436	preparation	426:436	preparation	426:436	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	8	10	theme	formulation	1263:1273	arg1	variables					1275:1283	the formulation variables	1259:1283	the formulation variables	1259:1283	The effects of the formulation variables and the composition of the optimized formulation were confirmed by a mixture design.
32235344	6	11	theme	colloidal	1081:1089	arg1	dispersion					1091:1100	the final colloidal dispersion	1071:1100	the final colloidal dispersion	1071:1100	As dependent variables (responses), nanoparticle dimensions and clotrimazole concentration in the final colloidal dispersion were considered.
32235344	2	12	theme	ionic	309:313	arg1	micelles					325:332	ionic polymeric micelles	309:332	ionic polymeric micelles based on chitosan and fatty acids	309:366	Previously, ionic polymeric micelles based on chitosan and fatty acids have been developed.
32235344	9	13	theme	Physicochemical	1370:1384	arg1	characterization					1386:1401	Physicochemical characterization	1370:1401	Physicochemical characterization of the optimized formulation	1370:1430	Physicochemical characterization of the optimized formulation was performed, confirming the ionic interaction between the polysaccharide and the HDA.
32235344	4	14	theme	hydrophobic	691:701	arg1	clotrimazole					720:731	clotrimazole	720:731	clotrimazole (CLO)	720:737	The HA-HDA polymeric micelles were loaded with a poorly soluble hydrophobic antifungal drug, clotrimazole (CLO).
32235344	4	14	theme	hydrophobic	691:701	arg1	drug					714:717	a poorly soluble hydrophobic antifungal drug	674:717	a poorly soluble hydrophobic antifungal drug	674:717	The HA-HDA polymeric micelles were loaded with a poorly soluble hydrophobic antifungal drug, clotrimazole (CLO).
32235344	5	15	theme	factors	829:835	arg1	cholesterol					874:884	cholesterol	874:884	cholesterol (CHOL%)	874:892	A 23 full factorial experimental design was used to evaluate the effect of the following factors: HA/HDA ratio from 1:0.25 to 1:0.75, cholesterol (CHOL%) as percentage of HA from 10% to 30%, and preparation temperature from 20 to 40 °C.
32235344	5	15	theme	factors	829:835	arg1	effect					805:810	the effect	801:810	the effect of the following factors	801:835	A 23 full factorial experimental design was used to evaluate the effect of the following factors: HA/HDA ratio from 1:0.25 to 1:0.75, cholesterol (CHOL%) as percentage of HA from 10% to 30%, and preparation temperature from 20 to 40 °C.
32235344	3	16	theme	carboxylic	538:547	arg1	DDA					595:597	DDA	595:597	DDA	595:597	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	16	theme	carboxylic	538:547	arg1	amine					588:592	dodecyl amine	580:592	dodecyl amine (DDA)	580:598	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	16	theme	carboxylic	538:547	arg1	amine					614:618	hexadecyl amine	604:618	hexadecyl amine (HDA)	604:624	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	16	theme	carboxylic	538:547	arg1	groups					549:554	HA carboxylic groups	535:554	HA carboxylic groups	535:554	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	16	theme	carboxylic	538:547	arg1	HDA					621:623	HDA	621:623	HDA	621:623	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	4	17	theme	polymeric	638:646	arg1	micelles					648:655	The HA-HDA polymeric micelles	627:655	The HA-HDA polymeric micelles	627:655	The HA-HDA polymeric micelles were loaded with a poorly soluble hydrophobic antifungal drug, clotrimazole (CLO).
32235344	5	18	from	°C	973:974	arg1	temperature					947:957	preparation temperature	935:957	preparation temperature from 20 to 40 °C	935:974	A 23 full factorial experimental design was used to evaluate the effect of the following factors: HA/HDA ratio from 1:0.25 to 1:0.75, cholesterol (CHOL%) as percentage of HA from 10% to 30%, and preparation temperature from 20 to 40 °C.
32235344	5	18	from	°C	973:974	arg1	percentage					897:906	percentage	897:906	percentage of HA from 10% to 30%	897:928	A 23 full factorial experimental design was used to evaluate the effect of the following factors: HA/HDA ratio from 1:0.25 to 1:0.75, cholesterol (CHOL%) as percentage of HA from 10% to 30%, and preparation temperature from 20 to 40 °C.
32235344	5	19	theme	full	745:748	arg1	design					773:778	A 23 full factorial experimental design	740:778	A 23 full factorial experimental design	740:778	A 23 full factorial experimental design was used to evaluate the effect of the following factors: HA/HDA ratio from 1:0.25 to 1:0.75, cholesterol (CHOL%) as percentage of HA from 10% to 30%, and preparation temperature from 20 to 40 °C.
32235344	6	20	theme	clotrimazole	1041:1052	arg1	concentration					1054:1066	clotrimazole concentration	1041:1066	clotrimazole concentration	1041:1066	As dependent variables (responses), nanoparticle dimensions and clotrimazole concentration in the final colloidal dispersion were considered.
32235344	6	20	theme	clotrimazole	1041:1052	arg1	variables					990:998	dependent variables	980:998	dependent variables (responses)	980:1010	As dependent variables (responses), nanoparticle dimensions and clotrimazole concentration in the final colloidal dispersion were considered.
32235344	6	20	theme	clotrimazole	1041:1052	arg1	dimensions					1026:1035	nanoparticle dimensions	1013:1035	nanoparticle dimensions	1013:1035	As dependent variables (responses), nanoparticle dimensions and clotrimazole concentration in the final colloidal dispersion were considered.
32235344	9	21	theme	formulation	1420:1430	arg1	characterization					1386:1401	Physicochemical characterization	1370:1401	Physicochemical characterization of the optimized formulation	1370:1430	Physicochemical characterization of the optimized formulation was performed, confirming the ionic interaction between the polysaccharide and the HDA.
32235344	3	22	theme	hyaluronic	462:471	arg1	HA					479:480	HA	479:480	HA	479:480	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	22	theme	hyaluronic	462:471	arg1	acid					473:476	hyaluronic acid	462:476	hyaluronic acid (HA) derivatives	462:493	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	1	23	contain	have	173:176	arg2	advantages					183:192	some advantages	178:192	some advantages as a carrier of poorly soluble lipophilic drugs thanks to their characteristic "core-shell" structure	178:294	Polymeric micelles based on amphiphilic polysaccharides have some advantages as a carrier of poorly soluble lipophilic drugs thanks to their characteristic "core-shell" structure.
32235344	1	23	contain	have	173:176	arg1	micelles					127:134	Polymeric micelles	117:134	Polymeric micelles based on amphiphilic polysaccharides	117:171	Polymeric micelles based on amphiphilic polysaccharides have some advantages as a carrier of poorly soluble lipophilic drugs thanks to their characteristic "core-shell" structure.
32235344	7	24	theme	composite	1220:1228	arg1	CCD					1238:1240	CCD	1238:1240	CCD	1238:1240	To optimize the drug final concentration, the design was therefore expanded into a rotatable central composite design (CCD).
32235344	7	24	theme	composite	1220:1228	arg1	design					1230:1235	a rotatable central composite design	1200:1235	a rotatable central composite design (CCD)	1200:1241	To optimize the drug final concentration, the design was therefore expanded into a rotatable central composite design (CCD).
32235344	5	25	theme	CHOL	887:890	arg1	cholesterol					874:884	cholesterol	874:884	cholesterol (CHOL%)	874:892	A 23 full factorial experimental design was used to evaluate the effect of the following factors: HA/HDA ratio from 1:0.25 to 1:0.75, cholesterol (CHOL%) as percentage of HA from 10% to 30%, and preparation temperature from 20 to 40 °C.
32235344	5	25	theme	CHOL	887:890	arg1	%					891:891	CHOL%	887:891	CHOL%	887:891	A 23 full factorial experimental design was used to evaluate the effect of the following factors: HA/HDA ratio from 1:0.25 to 1:0.75, cholesterol (CHOL%) as percentage of HA from 10% to 30%, and preparation temperature from 20 to 40 °C.
32235344	6	26	theme	nanoparticle	1013:1024	arg1	concentration					1054:1066	clotrimazole concentration	1041:1066	clotrimazole concentration	1041:1066	As dependent variables (responses), nanoparticle dimensions and clotrimazole concentration in the final colloidal dispersion were considered.
32235344	6	26	theme	nanoparticle	1013:1024	arg1	variables					990:998	dependent variables	980:998	dependent variables (responses)	980:1010	As dependent variables (responses), nanoparticle dimensions and clotrimazole concentration in the final colloidal dispersion were considered.
32235344	6	26	theme	nanoparticle	1013:1024	arg1	dimensions					1026:1035	nanoparticle dimensions	1013:1035	nanoparticle dimensions	1013:1035	As dependent variables (responses), nanoparticle dimensions and clotrimazole concentration in the final colloidal dispersion were considered.
32235344	3	27	theme	present	404:410	arg1	study					412:416	the present study	400:416	the present study	400:416	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	28	dep	preparation	426:436	arg1	the					422:424	the	422:424	the	422:424	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	5	29	from	1:0.75	866:871	arg1	ratio					845:849	HA/HDA ratio	838:849	HA/HDA ratio from 1:0.25 to 1:0.75	838:871	A 23 full factorial experimental design was used to evaluate the effect of the following factors: HA/HDA ratio from 1:0.25 to 1:0.75, cholesterol (CHOL%) as percentage of HA from 10% to 30%, and preparation temperature from 20 to 40 °C.
32235344	3	30	theme	dodecyl	580:586	arg1	DDA					595:597	DDA	595:597	DDA	595:597	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	30	theme	dodecyl	580:586	arg1	amine					588:592	dodecyl amine	580:592	dodecyl amine (DDA)	580:598	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	7	31	theme	rotatable	1202:1210	arg1	CCD					1238:1240	CCD	1238:1240	CCD	1238:1240	To optimize the drug final concentration, the design was therefore expanded into a rotatable central composite design (CCD).
32235344	7	31	theme	rotatable	1202:1210	arg1	design					1230:1235	a rotatable central composite design	1200:1235	a rotatable central composite design (CCD)	1200:1241	To optimize the drug final concentration, the design was therefore expanded into a rotatable central composite design (CCD).
32235344	5	32	theme	HA/HDA	838:843	arg1	ratio					845:849	HA/HDA ratio	838:849	HA/HDA ratio from 1:0.25 to 1:0.75	838:871	A 23 full factorial experimental design was used to evaluate the effect of the following factors: HA/HDA ratio from 1:0.25 to 1:0.75, cholesterol (CHOL%) as percentage of HA from 10% to 30%, and preparation temperature from 20 to 40 °C.
32235344	0	33	theme	Development	31:41	arg1	Design					0:5	Design	0:5	Design of Experiments-Assisted Development of Clotrimazole-Loaded Ionic Polymeric Micelles	0:89	Design of Experiments-Assisted Development of Clotrimazole-Loaded Ionic Polymeric Micelles Based on Hyaluronic Acid.
32235344	8	34	theme	mixture	1354:1360	arg1	design					1362:1367	a mixture design	1352:1367	a mixture design	1352:1367	The effects of the formulation variables and the composition of the optimized formulation were confirmed by a mixture design.
32235344	1	35	theme	Polymeric	117:125	arg1	micelles					127:134	Polymeric micelles	117:134	Polymeric micelles based on amphiphilic polysaccharides	117:171	Polymeric micelles based on amphiphilic polysaccharides have some advantages as a carrier of poorly soluble lipophilic drugs thanks to their characteristic "core-shell" structure.
32235344	1	36	theme	soluble	217:223	arg1	drugs					236:240	poorly soluble lipophilic drugs	210:240	poorly soluble lipophilic drugs thanks to their characteristic "core-shell" structure	210:294	Polymeric micelles based on amphiphilic polysaccharides have some advantages as a carrier of poorly soluble lipophilic drugs thanks to their characteristic "core-shell" structure.
32235344	0	37	theme	Experiments-Assisted	10:29	arg1	Development					31:41	Experiments-Assisted Development	10:41	Experiments-Assisted Development of Clotrimazole-Loaded Ionic Polymeric Micelles	10:89	Design of Experiments-Assisted Development of Clotrimazole-Loaded Ionic Polymeric Micelles Based on Hyaluronic Acid.
32235344	5	38	theme	HA	911:912	arg1	temperature					947:957	preparation temperature	935:957	preparation temperature from 20 to 40 °C	935:974	A 23 full factorial experimental design was used to evaluate the effect of the following factors: HA/HDA ratio from 1:0.25 to 1:0.75, cholesterol (CHOL%) as percentage of HA from 10% to 30%, and preparation temperature from 20 to 40 °C.
32235344	5	38	theme	HA	911:912	arg1	percentage					897:906	percentage	897:906	percentage of HA from 10% to 30%	897:928	A 23 full factorial experimental design was used to evaluate the effect of the following factors: HA/HDA ratio from 1:0.25 to 1:0.75, cholesterol (CHOL%) as percentage of HA from 10% to 30%, and preparation temperature from 20 to 40 °C.
32235344	6	39	theme	dependent	980:988	arg1	concentration					1054:1066	clotrimazole concentration	1041:1066	clotrimazole concentration	1041:1066	As dependent variables (responses), nanoparticle dimensions and clotrimazole concentration in the final colloidal dispersion were considered.
32235344	6	39	theme	dependent	980:988	arg1	responses					1001:1009	responses	1001:1009	responses	1001:1009	As dependent variables (responses), nanoparticle dimensions and clotrimazole concentration in the final colloidal dispersion were considered.
32235344	6	39	theme	dependent	980:988	arg1	variables					990:998	dependent variables	980:998	dependent variables (responses)	980:1010	As dependent variables (responses), nanoparticle dimensions and clotrimazole concentration in the final colloidal dispersion were considered.
32235344	6	39	theme	dependent	980:988	arg1	dimensions					1026:1035	nanoparticle dimensions	1013:1035	nanoparticle dimensions	1013:1035	As dependent variables (responses), nanoparticle dimensions and clotrimazole concentration in the final colloidal dispersion were considered.
32235344	5	40	from	%	928:928	arg1	temperature					947:957	preparation temperature	935:957	preparation temperature from 20 to 40 °C	935:974	A 23 full factorial experimental design was used to evaluate the effect of the following factors: HA/HDA ratio from 1:0.25 to 1:0.75, cholesterol (CHOL%) as percentage of HA from 10% to 30%, and preparation temperature from 20 to 40 °C.
32235344	5	40	from	%	928:928	arg1	HA					911:912	HA	911:912	HA from 10% to 30%	911:928	A 23 full factorial experimental design was used to evaluate the effect of the following factors: HA/HDA ratio from 1:0.25 to 1:0.75, cholesterol (CHOL%) as percentage of HA from 10% to 30%, and preparation temperature from 20 to 40 °C.
32235344	5	40	from	%	928:928	arg1	percentage					897:906	percentage	897:906	percentage of HA from 10% to 30%	897:928	A 23 full factorial experimental design was used to evaluate the effect of the following factors: HA/HDA ratio from 1:0.25 to 1:0.75, cholesterol (CHOL%) as percentage of HA from 10% to 30%, and preparation temperature from 20 to 40 °C.
32235344	1	41	theme	lipophilic	225:234	arg1	drugs					236:240	poorly soluble lipophilic drugs	210:240	poorly soluble lipophilic drugs thanks to their characteristic "core-shell" structure	210:294	Polymeric micelles based on amphiphilic polysaccharides have some advantages as a carrier of poorly soluble lipophilic drugs thanks to their characteristic "core-shell" structure.
32235344	0	42	theme	Clotrimazole-Loaded	46:64	arg1	Micelles					82:89	Clotrimazole-Loaded Ionic Polymeric Micelles	46:89	Clotrimazole-Loaded Ionic Polymeric Micelles	46:89	Design of Experiments-Assisted Development of Clotrimazole-Loaded Ionic Polymeric Micelles Based on Hyaluronic Acid.
32235344	3	43	dep	groups	549:554	arg1	the					531:533	the	531:533	the	531:533	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	44	theme	acid	473:476	arg1	derivatives					483:493	hyaluronic acid (HA) derivatives	462:493	hyaluronic acid (HA) derivatives	462:493	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	6	45	from	concentration	1054:1066	arg1	dispersion					1091:1100	the final colloidal dispersion	1071:1100	the final colloidal dispersion	1071:1100	As dependent variables (responses), nanoparticle dimensions and clotrimazole concentration in the final colloidal dispersion were considered.
32235344	1	46	theme	drugs	236:240	arg1	carrier					199:205	a carrier	197:205	a carrier of poorly soluble lipophilic drugs thanks to their characteristic "core-shell" structure	197:294	Polymeric micelles based on amphiphilic polysaccharides have some advantages as a carrier of poorly soluble lipophilic drugs thanks to their characteristic "core-shell" structure.
32235344	5	47	theme	following	819:827	arg1	factors					829:835	the following factors	815:835	the following factors	815:835	A 23 full factorial experimental design was used to evaluate the effect of the following factors: HA/HDA ratio from 1:0.25 to 1:0.75, cholesterol (CHOL%) as percentage of HA from 10% to 30%, and preparation temperature from 20 to 40 °C.
32235344	5	48	dep	1:0.75	866:871	arg1	to					863:864	to	863:864	to	863:864	A 23 full factorial experimental design was used to evaluate the effect of the following factors: HA/HDA ratio from 1:0.25 to 1:0.75, cholesterol (CHOL%) as percentage of HA from 10% to 30%, and preparation temperature from 20 to 40 °C.
32235344	5	49	theme	factorial	750:758	arg1	design					773:778	A 23 full factorial experimental design	740:778	A 23 full factorial experimental design	740:778	A 23 full factorial experimental design was used to evaluate the effect of the following factors: HA/HDA ratio from 1:0.25 to 1:0.75, cholesterol (CHOL%) as percentage of HA from 10% to 30%, and preparation temperature from 20 to 40 °C.
32235344	5	50	dep	40	970:971	arg1	to					967:968	to	967:968	to	967:968	A 23 full factorial experimental design was used to evaluate the effect of the following factors: HA/HDA ratio from 1:0.25 to 1:0.75, cholesterol (CHOL%) as percentage of HA from 10% to 30%, and preparation temperature from 20 to 40 °C.
32235344	1	51	theme	thanks	242:247	arg1	drugs					236:240	poorly soluble lipophilic drugs	210:240	poorly soluble lipophilic drugs thanks to their characteristic "core-shell" structure	210:294	Polymeric micelles based on amphiphilic polysaccharides have some advantages as a carrier of poorly soluble lipophilic drugs thanks to their characteristic "core-shell" structure.
32235344	0	52	theme	Polymeric	72:80	arg1	Micelles					82:89	Clotrimazole-Loaded Ionic Polymeric Micelles	46:89	Clotrimazole-Loaded Ionic Polymeric Micelles	46:89	Design of Experiments-Assisted Development of Clotrimazole-Loaded Ionic Polymeric Micelles Based on Hyaluronic Acid.
32235344	7	53	theme	final	1140:1144	arg1	concentration					1146:1158	the drug final concentration	1131:1158	the drug final concentration	1131:1158	To optimize the drug final concentration, the design was therefore expanded into a rotatable central composite design (CCD).
32235344	3	54	theme	amine	588:592	arg1	DDA					595:597	DDA	595:597	DDA	595:597	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	54	theme	amine	588:592	arg1	HDA					621:623	HDA	621:623	HDA	621:623	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	54	theme	amine	588:592	arg1	groups					570:575	the amine groups	560:575	the amine groups	560:575	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	54	theme	amine	588:592	arg1	amine					614:618	hexadecyl amine	604:618	hexadecyl amine (HDA)	604:624	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	54	theme	amine	588:592	arg1	groups					549:554	HA carboxylic groups	535:554	HA carboxylic groups	535:554	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	54	theme	amine	588:592	arg1	amine					588:592	dodecyl amine	580:592	dodecyl amine (DDA)	580:598	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	55	theme	direct	498:503	arg1	interaction					511:521	direct ionic interaction	498:521	direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA)	498:624	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	0	56	theme	Ionic	66:70	arg1	Micelles					82:89	Clotrimazole-Loaded Ionic Polymeric Micelles	46:89	Clotrimazole-Loaded Ionic Polymeric Micelles	46:89	Design of Experiments-Assisted Development of Clotrimazole-Loaded Ionic Polymeric Micelles Based on Hyaluronic Acid.
32235344	3	57	theme	derivatives	483:493	arg1	aim					393:395	The aim	389:395	The aim of the present study	389:416	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	57	theme	derivatives	483:493	arg1	characterization					442:457	characterization	442:457	characterization	442:457	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	57	theme	derivatives	483:493	arg1	preparation					426:436	preparation	426:436	preparation	426:436	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	9	58	theme	ionic	1462:1466	arg1	interaction					1468:1478	the ionic interaction	1458:1478	the ionic interaction between the polysaccharide and the HDA	1458:1517	Physicochemical characterization of the optimized formulation was performed, confirming the ionic interaction between the polysaccharide and the HDA.
32235344	5	59	theme	preparation	935:945	arg1	temperature					947:957	preparation temperature	935:957	preparation temperature from 20 to 40 °C	935:974	A 23 full factorial experimental design was used to evaluate the effect of the following factors: HA/HDA ratio from 1:0.25 to 1:0.75, cholesterol (CHOL%) as percentage of HA from 10% to 30%, and preparation temperature from 20 to 40 °C.
32235344	6	60	from	dimensions	1026:1035	arg1	dispersion					1091:1100	the final colloidal dispersion	1071:1100	the final colloidal dispersion	1071:1100	As dependent variables (responses), nanoparticle dimensions and clotrimazole concentration in the final colloidal dispersion were considered.
32235344	3	61	theme	ionic	505:509	arg1	interaction					511:521	direct ionic interaction	498:521	direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA)	498:624	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	5	62	theme	30	926:927	arg1	%					921:921	%	921:921	%	921:921	A 23 full factorial experimental design was used to evaluate the effect of the following factors: HA/HDA ratio from 1:0.25 to 1:0.75, cholesterol (CHOL%) as percentage of HA from 10% to 30%, and preparation temperature from 20 to 40 °C.
32235344	5	63	used	used	784:787	arg2	design					773:778	A 23 full factorial experimental design	740:778	A 23 full factorial experimental design	740:778	A 23 full factorial experimental design was used to evaluate the effect of the following factors: HA/HDA ratio from 1:0.25 to 1:0.75, cholesterol (CHOL%) as percentage of HA from 10% to 30%, and preparation temperature from 20 to 40 °C.
32235344	5	64	theme	10	919:920	arg1	%					921:921	%	921:921	%	921:921	A 23 full factorial experimental design was used to evaluate the effect of the following factors: HA/HDA ratio from 1:0.25 to 1:0.75, cholesterol (CHOL%) as percentage of HA from 10% to 30%, and preparation temperature from 20 to 40 °C.
32235344	3	65	theme	amine	614:618	arg1	DDA					595:597	DDA	595:597	DDA	595:597	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	65	theme	amine	614:618	arg1	HDA					621:623	HDA	621:623	HDA	621:623	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	65	theme	amine	614:618	arg1	groups					570:575	the amine groups	560:575	the amine groups	560:575	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	65	theme	amine	614:618	arg1	amine					614:618	hexadecyl amine	604:618	hexadecyl amine (HDA)	604:624	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	65	theme	amine	614:618	arg1	groups					549:554	HA carboxylic groups	535:554	HA carboxylic groups	535:554	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	65	theme	amine	614:618	arg1	amine					588:592	dodecyl amine	580:592	dodecyl amine (DDA)	580:598	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	1	66	theme	amphiphilic	145:155	arg1	polysaccharides					157:171	amphiphilic polysaccharides	145:171	amphiphilic polysaccharides	145:171	Polymeric micelles based on amphiphilic polysaccharides have some advantages as a carrier of poorly soluble lipophilic drugs thanks to their characteristic "core-shell" structure.
32235344	3	67	theme	amine	564:568	arg1	DDA					595:597	DDA	595:597	DDA	595:597	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	67	theme	amine	564:568	arg1	groups					570:575	the amine groups	560:575	the amine groups	560:575	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	67	theme	amine	564:568	arg1	amine					588:592	dodecyl amine	580:592	dodecyl amine (DDA)	580:598	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	67	theme	amine	564:568	arg1	amine					614:618	hexadecyl amine	604:618	hexadecyl amine (HDA)	604:624	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	67	theme	amine	564:568	arg1	HDA					621:623	HDA	621:623	HDA	621:623	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	5	68	dep	effect	805:810	arg1	ratio					845:849	HA/HDA ratio	838:849	HA/HDA ratio from 1:0.25 to 1:0.75	838:871	A 23 full factorial experimental design was used to evaluate the effect of the following factors: HA/HDA ratio from 1:0.25 to 1:0.75, cholesterol (CHOL%) as percentage of HA from 10% to 30%, and preparation temperature from 20 to 40 °C.
32235344	8	69	theme	optimized	1312:1320	arg1	formulation					1322:1332	the optimized formulation	1308:1332	the optimized formulation	1308:1332	The effects of the formulation variables and the composition of the optimized formulation were confirmed by a mixture design.
32235344	6	70	theme	final	1075:1079	arg1	dispersion					1091:1100	the final colloidal dispersion	1071:1100	the final colloidal dispersion	1071:1100	As dependent variables (responses), nanoparticle dimensions and clotrimazole concentration in the final colloidal dispersion were considered.
32235344	4	71	theme	antifungal	703:712	arg1	clotrimazole					720:731	clotrimazole	720:731	clotrimazole (CLO)	720:737	The HA-HDA polymeric micelles were loaded with a poorly soluble hydrophobic antifungal drug, clotrimazole (CLO).
32235344	4	71	theme	antifungal	703:712	arg1	drug					714:717	a poorly soluble hydrophobic antifungal drug	674:717	a poorly soluble hydrophobic antifungal drug	674:717	The HA-HDA polymeric micelles were loaded with a poorly soluble hydrophobic antifungal drug, clotrimazole (CLO).
32235344	2	72	theme	fatty	356:360	arg1	acids					362:366	fatty acids	356:366	fatty acids	356:366	Previously, ionic polymeric micelles based on chitosan and fatty acids have been developed.
32235344	7	73	theme	drug	1135:1138	arg1	concentration					1146:1158	the drug final concentration	1131:1158	the drug final concentration	1131:1158	To optimize the drug final concentration, the design was therefore expanded into a rotatable central composite design (CCD).
32235344	5	74	dep	%	921:921	arg1	to					923:924	to	923:924	to	923:924	A 23 full factorial experimental design was used to evaluate the effect of the following factors: HA/HDA ratio from 1:0.25 to 1:0.75, cholesterol (CHOL%) as percentage of HA from 10% to 30%, and preparation temperature from 20 to 40 °C.
32235344	7	75	theme	central	1212:1218	arg1	CCD					1238:1240	CCD	1238:1240	CCD	1238:1240	To optimize the drug final concentration, the design was therefore expanded into a rotatable central composite design (CCD).
32235344	7	75	theme	central	1212:1218	arg1	design					1230:1235	a rotatable central composite design	1200:1235	a rotatable central composite design (CCD)	1200:1241	To optimize the drug final concentration, the design was therefore expanded into a rotatable central composite design (CCD).
32235344	9	76	theme	optimized	1410:1418	arg1	formulation					1420:1430	the optimized formulation	1406:1430	the optimized formulation	1406:1430	Physicochemical characterization of the optimized formulation was performed, confirming the ionic interaction between the polysaccharide and the HDA.
32235344	4	77	theme	HA-HDA	631:636	arg1	micelles					648:655	The HA-HDA polymeric micelles	627:655	The HA-HDA polymeric micelles	627:655	The HA-HDA polymeric micelles were loaded with a poorly soluble hydrophobic antifungal drug, clotrimazole (CLO).
32235344	1	78	theme	characteristic	258:271	arg1	structure					286:294	their characteristic "core-shell" structure	252:294	their characteristic "core-shell" structure	252:294	Polymeric micelles based on amphiphilic polysaccharides have some advantages as a carrier of poorly soluble lipophilic drugs thanks to their characteristic "core-shell" structure.
32235344	0	79	theme	Micelles	82:89	arg1	Development					31:41	Experiments-Assisted Development	10:41	Experiments-Assisted Development of Clotrimazole-Loaded Ionic Polymeric Micelles	10:89	Design of Experiments-Assisted Development of Clotrimazole-Loaded Ionic Polymeric Micelles Based on Hyaluronic Acid.
32235344	3	80	theme	hexadecyl	604:612	arg1	HDA					621:623	HDA	621:623	HDA	621:623	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	80	theme	hexadecyl	604:612	arg1	amine					614:618	hexadecyl amine	604:618	hexadecyl amine (HDA)	604:624	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	81	theme	HA	535:536	arg1	DDA					595:597	DDA	595:597	DDA	595:597	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	81	theme	HA	535:536	arg1	amine					588:592	dodecyl amine	580:592	dodecyl amine (DDA)	580:598	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	81	theme	HA	535:536	arg1	amine					614:618	hexadecyl amine	604:618	hexadecyl amine (HDA)	604:624	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	81	theme	HA	535:536	arg1	groups					549:554	HA carboxylic groups	535:554	HA carboxylic groups	535:554	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	3	81	theme	HA	535:536	arg1	HDA					621:623	HDA	621:623	HDA	621:623	The aim of the present study was the preparation and characterization of hyaluronic acid (HA) derivatives by direct ionic interaction between the HA carboxylic groups and the amine groups of dodecyl amine (DDA) and hexadecyl amine (HDA).
32235344	4	82	theme	soluble	683:689	arg1	clotrimazole					720:731	clotrimazole	720:731	clotrimazole (CLO)	720:737	The HA-HDA polymeric micelles were loaded with a poorly soluble hydrophobic antifungal drug, clotrimazole (CLO).
32235344	4	82	theme	soluble	683:689	arg1	drug					714:717	a poorly soluble hydrophobic antifungal drug	674:717	a poorly soluble hydrophobic antifungal drug	674:717	The HA-HDA polymeric micelles were loaded with a poorly soluble hydrophobic antifungal drug, clotrimazole (CLO).
33399997	7	0	theme	females	1310:1316	arg1	hippocampus					1295:1305	the dorsal hippocampus	1284:1305	the dorsal hippocampus of females and males independently on the NPC	1284:1351	The number of GAD67 expressing neurons was similar in the dorsal hippocampus of females and males independently on the NPC.
33399997	4	1	theme	juvenile	770:777	arg1	rats					795:798	juvenile male and female rats	770:798	juvenile male and female rats using immunofluorescent histochemistry	770:837	We studied effects of NPC on microglia and GABAergic neuronal population of the dorsal hippocampus in juvenile male and female rats using immunofluorescent histochemistry.
33399997	4	2	from	microglia	697:705	arg1	rats					795:798	juvenile male and female rats	770:798	juvenile male and female rats using immunofluorescent histochemistry	770:837	We studied effects of NPC on microglia and GABAergic neuronal population of the dorsal hippocampus in juvenile male and female rats using immunofluorescent histochemistry.
33399997	9	3	from	subpopulation	1647:1659	arg1	hippocampus					1689:1699	the hippocampus	1685:1699	the hippocampus of rats of either sex	1685:1721	NPC did not affect the parvalbumin-positive subpopulation of GABAergic neurons in the hippocampus of rats of either sex.
33399997	10	4	theme	GABAergic	1776:1784	arg1	composition					1806:1816	GABAergic neuronal population composition	1776:1816	GABAergic neuronal population composition	1776:1816	These data suggest that NPC-induced modification of GABAergic neuronal population composition under the proinflammatory conditions is involved in the maintenance of excitation/inhibition homeostatic balance in the hippocampus.
33399997	4	5	theme	NPC	690:692	arg1	effects					679:685	effects	679:685	effects of NPC on microglia and GABAergic neuronal population of the dorsal hippocampus in juvenile male and female rats using immunofluorescent histochemistry	679:837	We studied effects of NPC on microglia and GABAergic neuronal population of the dorsal hippocampus in juvenile male and female rats using immunofluorescent histochemistry.
33399997	1	6	from	adults	312:317	arg1	development					272:282	the development	268:282	the development of psychiatric disorders in adults	268:317	Neonatal proinflammatory challenge (NPC) may contribute to the development of psychiatric disorders in adults.
33399997	0	7	theme	Neonatal	0:7	arg1	challenge					25:33	Neonatal proinflammatory challenge	0:33	Neonatal proinflammatory challenge	0:33	Neonatal proinflammatory challenge evokes a microglial response and affects the ratio between subtypes of GABAergic interneurons in the hippocampus of juvenile rats: sex-dependent and sex-independent effects.
33399997	5	8	theme	proteins	917:924	arg1	markers					991:997	quantitative markers	978:997	quantitative markers of GABAergic interneurons and their specific subpopulations	978:1057	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin were used as quantitative markers of GABAergic interneurons and their specific subpopulations, respectively.
33399997	5	8	theme	proteins	917:924	arg1	expression					844:853	The expression	840:853	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin	840:963	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin were used as quantitative markers of GABAergic interneurons and their specific subpopulations, respectively.
33399997	1	9	from	disorders	299:307	arg1	adults					312:317	adults	312:317	adults	312:317	Neonatal proinflammatory challenge (NPC) may contribute to the development of psychiatric disorders in adults.
33399997	2	10	theme	double	322:327	arg1	exposure					329:336	A double exposure	320:336	A double exposure of neonatal rats to lipopolysaccharide, a component of cellular wall of gram-negative bacteria, on postnatal days 3 and 5	320:458	A double exposure of neonatal rats to lipopolysaccharide, a component of cellular wall of gram-negative bacteria, on postnatal days 3 and 5 provokes the development of depressive- and anxiety-like behaviors.
33399997	4	11	from	effects	679:685	arg1	population					730:739	GABAergic neuronal population	711:739	GABAergic neuronal population of the dorsal hippocampus in juvenile male and female rats using immunofluorescent histochemistry	711:837	We studied effects of NPC on microglia and GABAergic neuronal population of the dorsal hippocampus in juvenile male and female rats using immunofluorescent histochemistry.
33399997	4	11	from	effects	679:685	arg1	microglia					697:705	microglia	697:705	microglia	697:705	We studied effects of NPC on microglia and GABAergic neuronal population of the dorsal hippocampus in juvenile male and female rats using immunofluorescent histochemistry.
33399997	1	12	theme	proinflammatory	218:232	arg1	NPC					245:247	NPC	245:247	NPC	245:247	Neonatal proinflammatory challenge (NPC) may contribute to the development of psychiatric disorders in adults.
33399997	1	12	theme	proinflammatory	218:232	arg1	challenge					234:242	Neonatal proinflammatory challenge	209:242	Neonatal proinflammatory challenge (NPC)	209:248	Neonatal proinflammatory challenge (NPC) may contribute to the development of psychiatric disorders in adults.
33399997	10	13	theme	NPC-induced	1748:1758	arg1	modification					1760:1771	NPC-induced modification	1748:1771	NPC-induced modification of GABAergic neuronal population composition under the proinflammatory conditions	1748:1853	These data suggest that NPC-induced modification of GABAergic neuronal population composition under the proinflammatory conditions is involved in the maintenance of excitation/inhibition homeostatic balance in the hippocampus.
33399997	0	14	theme	juvenile	151:158	arg1	rats					160:163	juvenile rats	151:163	juvenile rats	151:163	Neonatal proinflammatory challenge evokes a microglial response and affects the ratio between subtypes of GABAergic interneurons in the hippocampus of juvenile rats: sex-dependent and sex-independent effects.
33399997	4	15	theme	hippocampus	755:765	arg1	population					730:739	GABAergic neuronal population	711:739	GABAergic neuronal population of the dorsal hippocampus in juvenile male and female rats using immunofluorescent histochemistry	711:837	We studied effects of NPC on microglia and GABAergic neuronal population of the dorsal hippocampus in juvenile male and female rats using immunofluorescent histochemistry.
33399997	4	15	theme	hippocampus	755:765	arg1	microglia					697:705	microglia	697:705	microglia	697:705	We studied effects of NPC on microglia and GABAergic neuronal population of the dorsal hippocampus in juvenile male and female rats using immunofluorescent histochemistry.
33399997	5	16	theme	quantitative	978:989	arg1	markers					991:997	quantitative markers	978:997	quantitative markers of GABAergic interneurons and their specific subpopulations	978:1057	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin were used as quantitative markers of GABAergic interneurons and their specific subpopulations, respectively.
33399997	5	16	theme	quantitative	978:989	arg1	expression					844:853	The expression	840:853	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin	840:963	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin were used as quantitative markers of GABAergic interneurons and their specific subpopulations, respectively.
33399997	8	17	theme	calbindin-immunoreactive	1369:1392	arg1	neurons					1409:1415	calbindin-immunoreactive GAD67-positive neurons	1369:1415	calbindin-immunoreactive GAD67-positive neurons	1369:1415	The portion of calbindin-immunoreactive GAD67-positive neurons significantly increased while the portion of calretinin-immunoreactive GAD67-positive neurons significantly decreased in the CA1 field of rats exposed to NPC independently on their sex.
33399997	2	18	theme	anxiety-like	504:515	arg1	behaviors					517:525	anxiety-like behaviors	504:525	anxiety-like behaviors	504:525	A double exposure of neonatal rats to lipopolysaccharide, a component of cellular wall of gram-negative bacteria, on postnatal days 3 and 5 provokes the development of depressive- and anxiety-like behaviors.
33399997	0	19	from	subtypes	94:101	arg1	hippocampus					136:146	the hippocampus	132:146	the hippocampus of juvenile rats	132:163	Neonatal proinflammatory challenge evokes a microglial response and affects the ratio between subtypes of GABAergic interneurons in the hippocampus of juvenile rats: sex-dependent and sex-independent effects.
33399997	8	20	theme	neurons	1409:1415	arg1	portion					1358:1364	The portion	1354:1364	The portion of calbindin-immunoreactive GAD67-positive neurons	1354:1415	The portion of calbindin-immunoreactive GAD67-positive neurons significantly increased while the portion of calretinin-immunoreactive GAD67-positive neurons significantly decreased in the CA1 field of rats exposed to NPC independently on their sex.
33399997	2	21	theme	depressive-	488:498	arg1	development					473:483	the development	469:483	the development of depressive- and anxiety-like behaviors	469:525	A double exposure of neonatal rats to lipopolysaccharide, a component of cellular wall of gram-negative bacteria, on postnatal days 3 and 5 provokes the development of depressive- and anxiety-like behaviors.
33399997	7	22	theme	dorsal	1288:1293	arg1	hippocampus					1295:1305	the dorsal hippocampus	1284:1305	the dorsal hippocampus of females and males independently on the NPC	1284:1351	The number of GAD67 expressing neurons was similar in the dorsal hippocampus of females and males independently on the NPC.
33399997	9	23	theme	GABAergic	1664:1672	arg1	neurons					1674:1680	GABAergic neurons	1664:1680	GABAergic neurons	1664:1680	NPC did not affect the parvalbumin-positive subpopulation of GABAergic neurons in the hippocampus of rats of either sex.
33399997	8	24	theme	neurons	1503:1509	arg1	portion					1451:1457	the portion	1447:1457	the portion of calretinin-immunoreactive GAD67-positive neurons	1447:1509	The portion of calbindin-immunoreactive GAD67-positive neurons significantly increased while the portion of calretinin-immunoreactive GAD67-positive neurons significantly decreased in the CA1 field of rats exposed to NPC independently on their sex.
33399997	2	25	from	5	458:458	arg1	wall					402:405	cellular wall	393:405	cellular wall of gram-negative bacteria, on postnatal days 3 and 5	393:458	A double exposure of neonatal rats to lipopolysaccharide, a component of cellular wall of gram-negative bacteria, on postnatal days 3 and 5 provokes the development of depressive- and anxiety-like behaviors.
33399997	4	26	theme	immunofluorescent	806:822	arg1	histochemistry					824:837	immunofluorescent histochemistry	806:837	immunofluorescent histochemistry	806:837	We studied effects of NPC on microglia and GABAergic neuronal population of the dorsal hippocampus in juvenile male and female rats using immunofluorescent histochemistry.
33399997	10	27	theme	population	1795:1804	arg1	composition					1806:1816	GABAergic neuronal population composition	1776:1816	GABAergic neuronal population composition	1776:1816	These data suggest that NPC-induced modification of GABAergic neuronal population composition under the proinflammatory conditions is involved in the maintenance of excitation/inhibition homeostatic balance in the hippocampus.
33399997	0	28	theme	GABAergic	106:114	arg1	interneurons					116:127	GABAergic interneurons	106:127	GABAergic interneurons in the hippocampus of juvenile rats	106:163	Neonatal proinflammatory challenge evokes a microglial response and affects the ratio between subtypes of GABAergic interneurons in the hippocampus of juvenile rats: sex-dependent and sex-independent effects.
33399997	5	29	theme	subpopulations	1044:1057	arg1	markers					991:997	quantitative markers	978:997	quantitative markers of GABAergic interneurons and their specific subpopulations	978:1057	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin were used as quantitative markers of GABAergic interneurons and their specific subpopulations, respectively.
33399997	5	29	theme	subpopulations	1044:1057	arg1	expression					844:853	The expression	840:853	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin	840:963	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin were used as quantitative markers of GABAergic interneurons and their specific subpopulations, respectively.
33399997	8	30	theme	GAD67-positive	1488:1501	arg1	neurons					1503:1509	calretinin-immunoreactive GAD67-positive neurons	1462:1509	calretinin-immunoreactive GAD67-positive neurons	1462:1509	The portion of calbindin-immunoreactive GAD67-positive neurons significantly increased while the portion of calretinin-immunoreactive GAD67-positive neurons significantly decreased in the CA1 field of rats exposed to NPC independently on their sex.
33399997	3	31	from	adolescence	655:665	arg1	evident					639:645	evident	639:645	evident	639:645	NPC impairs neuroplasticity and cognition in adult animals, significant modifications of neuroplasticity being evident even in adolescence.
33399997	2	32	theme	cellular	393:400	arg1	wall					402:405	cellular wall	393:405	cellular wall of gram-negative bacteria, on postnatal days 3 and 5	393:458	A double exposure of neonatal rats to lipopolysaccharide, a component of cellular wall of gram-negative bacteria, on postnatal days 3 and 5 provokes the development of depressive- and anxiety-like behaviors.
33399997	5	33	theme	glutamic	858:865	arg1	acid					867:870	glutamic acid decarboxylase-67	858:887	glutamic acid decarboxylase-67 (GAD67)	858:895	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin were used as quantitative markers of GABAergic interneurons and their specific subpopulations, respectively.
33399997	5	33	theme	glutamic	858:865	arg1	GAD67					890:894	GAD67	890:894	GAD67	890:894	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin were used as quantitative markers of GABAergic interneurons and their specific subpopulations, respectively.
33399997	1	34	theme	psychiatric	287:297	arg1	disorders					299:307	psychiatric disorders	287:307	psychiatric disorders in adults	287:317	Neonatal proinflammatory challenge (NPC) may contribute to the development of psychiatric disorders in adults.
33399997	9	35	theme	rats	1704:1707	arg1	hippocampus					1689:1699	the hippocampus	1685:1699	the hippocampus of rats of either sex	1685:1721	NPC did not affect the parvalbumin-positive subpopulation of GABAergic neurons in the hippocampus of rats of either sex.
33399997	10	36	theme	proinflammatory	1828:1842	arg1	conditions					1844:1853	the proinflammatory conditions	1824:1853	the proinflammatory conditions	1824:1853	These data suggest that NPC-induced modification of GABAergic neuronal population composition under the proinflammatory conditions is involved in the maintenance of excitation/inhibition homeostatic balance in the hippocampus.
33399997	5	37	theme	interneurons	1012:1023	arg1	markers					991:997	quantitative markers	978:997	quantitative markers of GABAergic interneurons and their specific subpopulations	978:1057	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin were used as quantitative markers of GABAergic interneurons and their specific subpopulations, respectively.
33399997	5	37	theme	interneurons	1012:1023	arg1	expression					844:853	The expression	840:853	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin	840:963	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin were used as quantitative markers of GABAergic interneurons and their specific subpopulations, respectively.
33399997	6	38	theme	inflammatory	1130:1141	arg1	activation					1143:1152	inflammatory activation	1130:1152	inflammatory activation mostly expressed in CA3 field	1130:1182	NPC induced changes of microglial morphology indicating inflammatory activation mostly expressed in CA3 field; the effect was similar in males and females.
33399997	7	39	theme	expressing	1250:1259	arg1	neurons					1261:1267	GAD67 expressing neurons	1244:1267	GAD67 expressing neurons	1244:1267	The number of GAD67 expressing neurons was similar in the dorsal hippocampus of females and males independently on the NPC.
33399997	2	40	theme	bacteria	424:431	arg1	wall					402:405	cellular wall	393:405	cellular wall of gram-negative bacteria, on postnatal days 3 and 5	393:458	A double exposure of neonatal rats to lipopolysaccharide, a component of cellular wall of gram-negative bacteria, on postnatal days 3 and 5 provokes the development of depressive- and anxiety-like behaviors.
33399997	6	41	theme	morphology	1108:1117	arg1	changes					1086:1092	changes	1086:1092	changes of microglial morphology	1086:1117	NPC induced changes of microglial morphology indicating inflammatory activation mostly expressed in CA3 field; the effect was similar in males and females.
33399997	8	42	theme	CA1	1542:1544	arg1	field					1546:1550	the CA1 field	1538:1550	the CA1 field of rats exposed to NPC	1538:1573	The portion of calbindin-immunoreactive GAD67-positive neurons significantly increased while the portion of calretinin-immunoreactive GAD67-positive neurons significantly decreased in the CA1 field of rats exposed to NPC independently on their sex.
33399997	2	43	theme	neonatal	341:348	arg1	rats					350:353	neonatal rats	341:353	neonatal rats	341:353	A double exposure of neonatal rats to lipopolysaccharide, a component of cellular wall of gram-negative bacteria, on postnatal days 3 and 5 provokes the development of depressive- and anxiety-like behaviors.
33399997	10	44	theme	homeostatic	1911:1921	arg1	balance					1923:1929	excitation/inhibition homeostatic balance	1889:1929	excitation/inhibition homeostatic balance	1889:1929	These data suggest that NPC-induced modification of GABAergic neuronal population composition under the proinflammatory conditions is involved in the maintenance of excitation/inhibition homeostatic balance in the hippocampus.
33399997	2	45	from	bacteria	424:431	arg1	days					447:450	postnatal days 3 and 5	437:458	days	447:450	A double exposure of neonatal rats to lipopolysaccharide, a component of cellular wall of gram-negative bacteria, on postnatal days 3 and 5 provokes the development of depressive- and anxiety-like behaviors.
33399997	2	45	from	bacteria	424:431	arg1	5					458:458	5	458:458	5	458:458	A double exposure of neonatal rats to lipopolysaccharide, a component of cellular wall of gram-negative bacteria, on postnatal days 3 and 5 provokes the development of depressive- and anxiety-like behaviors.
33399997	0	46	from	hippocampus	136:146	arg1	subtypes					94:101	subtypes	94:101	subtypes of GABAergic interneurons in the hippocampus of juvenile rats	94:163	Neonatal proinflammatory challenge evokes a microglial response and affects the ratio between subtypes of GABAergic interneurons in the hippocampus of juvenile rats: sex-dependent and sex-independent effects.
33399997	10	47	theme	composition	1806:1816	arg1	modification					1760:1771	NPC-induced modification	1748:1771	NPC-induced modification of GABAergic neuronal population composition under the proinflammatory conditions	1748:1853	These data suggest that NPC-induced modification of GABAergic neuronal population composition under the proinflammatory conditions is involved in the maintenance of excitation/inhibition homeostatic balance in the hippocampus.
33399997	10	48	theme	excitation/inhibition	1889:1909	arg1	balance					1923:1929	excitation/inhibition homeostatic balance	1889:1929	excitation/inhibition homeostatic balance	1889:1929	These data suggest that NPC-induced modification of GABAergic neuronal population composition under the proinflammatory conditions is involved in the maintenance of excitation/inhibition homeostatic balance in the hippocampus.
33399997	7	49	theme	neurons	1261:1267	arg1	similar					1273:1279	similar	1273:1279	similar	1273:1279	The number of GAD67 expressing neurons was similar in the dorsal hippocampus of females and males independently on the NPC.
33399997	7	49	theme	neurons	1261:1267	arg1	number					1234:1239	The number	1230:1239	The number of GAD67 expressing neurons	1230:1267	The number of GAD67 expressing neurons was similar in the dorsal hippocampus of females and males independently on the NPC.
33399997	0	50	theme	sex-independent	184:198	arg1	effects					200:206	sex-dependent and sex-independent effects	166:206	sex-dependent and sex-independent effects	166:206	Neonatal proinflammatory challenge evokes a microglial response and affects the ratio between subtypes of GABAergic interneurons in the hippocampus of juvenile rats: sex-dependent and sex-independent effects.
33399997	10	51	theme	balance	1923:1929	arg1	maintenance					1874:1884	the maintenance	1870:1884	the maintenance of excitation/inhibition homeostatic balance in the hippocampus	1870:1948	These data suggest that NPC-induced modification of GABAergic neuronal population composition under the proinflammatory conditions is involved in the maintenance of excitation/inhibition homeostatic balance in the hippocampus.
33399997	5	52	theme	calcium-binding	901:915	arg1	proteins					917:924	calcium-binding proteins	901:924	calcium-binding proteins calretinin, calbindin, and parvalbumin	901:963	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin were used as quantitative markers of GABAergic interneurons and their specific subpopulations, respectively.
33399997	5	52	theme	calcium-binding	901:915	arg1	calbindin					938:946	calbindin	938:946	calbindin	938:946	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin were used as quantitative markers of GABAergic interneurons and their specific subpopulations, respectively.
33399997	5	52	theme	calcium-binding	901:915	arg1	parvalbumin					953:963	parvalbumin	953:963	parvalbumin	953:963	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin were used as quantitative markers of GABAergic interneurons and their specific subpopulations, respectively.
33399997	5	52	theme	calcium-binding	901:915	arg1	calretinin					926:935	calretinin	926:935	calretinin	926:935	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin were used as quantitative markers of GABAergic interneurons and their specific subpopulations, respectively.
33399997	10	53	from	maintenance	1874:1884	arg1	hippocampus					1938:1948	the hippocampus	1934:1948	the hippocampus	1934:1948	These data suggest that NPC-induced modification of GABAergic neuronal population composition under the proinflammatory conditions is involved in the maintenance of excitation/inhibition homeostatic balance in the hippocampus.
33399997	1	54	from	development	272:282	arg1	adults					312:317	adults	312:317	adults	312:317	Neonatal proinflammatory challenge (NPC) may contribute to the development of psychiatric disorders in adults.
33399997	3	55	theme	adult	573:577	arg1	animals					579:585	adult animals	573:585	adult animals	573:585	NPC impairs neuroplasticity and cognition in adult animals, significant modifications of neuroplasticity being evident even in adolescence.
33399997	1	56	theme	Neonatal	209:216	arg1	NPC					245:247	NPC	245:247	NPC	245:247	Neonatal proinflammatory challenge (NPC) may contribute to the development of psychiatric disorders in adults.
33399997	1	56	theme	Neonatal	209:216	arg1	challenge					234:242	Neonatal proinflammatory challenge	209:242	Neonatal proinflammatory challenge (NPC)	209:248	Neonatal proinflammatory challenge (NPC) may contribute to the development of psychiatric disorders in adults.
33399997	3	57	theme	significant	588:598	arg1	modifications					600:612	significant modifications	588:612	significant modifications of neuroplasticity	588:631	NPC impairs neuroplasticity and cognition in adult animals, significant modifications of neuroplasticity being evident even in adolescence.
33399997	3	58	theme	neuroplasticity	617:631	arg1	modifications					600:612	significant modifications	588:612	significant modifications of neuroplasticity	588:631	NPC impairs neuroplasticity and cognition in adult animals, significant modifications of neuroplasticity being evident even in adolescence.
33399997	7	59	from	similar	1273:1279	arg1	hippocampus					1295:1305	the dorsal hippocampus	1284:1305	the dorsal hippocampus of females and males independently on the NPC	1284:1351	The number of GAD67 expressing neurons was similar in the dorsal hippocampus of females and males independently on the NPC.
33399997	0	60	theme	rats	160:163	arg1	hippocampus					136:146	the hippocampus	132:146	the hippocampus of juvenile rats	132:163	Neonatal proinflammatory challenge evokes a microglial response and affects the ratio between subtypes of GABAergic interneurons in the hippocampus of juvenile rats: sex-dependent and sex-independent effects.
33399997	0	61	from	interneurons	116:127	arg1	hippocampus					136:146	the hippocampus	132:146	the hippocampus of juvenile rats	132:163	Neonatal proinflammatory challenge evokes a microglial response and affects the ratio between subtypes of GABAergic interneurons in the hippocampus of juvenile rats: sex-dependent and sex-independent effects.
33399997	0	62	theme	sex-dependent	166:178	arg1	effects					200:206	sex-dependent and sex-independent effects	166:206	sex-dependent and sex-independent effects	166:206	Neonatal proinflammatory challenge evokes a microglial response and affects the ratio between subtypes of GABAergic interneurons in the hippocampus of juvenile rats: sex-dependent and sex-independent effects.
33399997	6	63	theme	microglial	1097:1106	arg1	morphology					1108:1117	microglial morphology	1097:1117	microglial morphology	1097:1117	NPC induced changes of microglial morphology indicating inflammatory activation mostly expressed in CA3 field; the effect was similar in males and females.
33399997	7	64	from	hippocampus	1295:1305	arg1	similar					1273:1279	similar	1273:1279	similar	1273:1279	The number of GAD67 expressing neurons was similar in the dorsal hippocampus of females and males independently on the NPC.
33399997	7	64	from	hippocampus	1295:1305	arg1	NPC					1349:1351	the NPC	1345:1351	the NPC	1345:1351	The number of GAD67 expressing neurons was similar in the dorsal hippocampus of females and males independently on the NPC.
33399997	7	64	from	hippocampus	1295:1305	arg1	number					1234:1239	The number	1230:1239	The number of GAD67 expressing neurons	1230:1267	The number of GAD67 expressing neurons was similar in the dorsal hippocampus of females and males independently on the NPC.
33399997	4	65	theme	dorsal	748:753	arg1	hippocampus					755:765	the dorsal hippocampus	744:765	the dorsal hippocampus	744:765	We studied effects of NPC on microglia and GABAergic neuronal population of the dorsal hippocampus in juvenile male and female rats using immunofluorescent histochemistry.
33399997	5	66	used	used	970:973	arg2	markers					991:997	quantitative markers	978:997	quantitative markers of GABAergic interneurons and their specific subpopulations	978:1057	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin were used as quantitative markers of GABAergic interneurons and their specific subpopulations, respectively.
33399997	5	66	used	used	970:973	arg2	expression					844:853	The expression	840:853	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin	840:963	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin were used as quantitative markers of GABAergic interneurons and their specific subpopulations, respectively.
33399997	9	67	theme	parvalbumin-positive	1626:1645	arg1	subpopulation					1647:1659	the parvalbumin-positive subpopulation	1622:1659	the parvalbumin-positive subpopulation of GABAergic neurons in the hippocampus of rats of either sex	1622:1721	NPC did not affect the parvalbumin-positive subpopulation of GABAergic neurons in the hippocampus of rats of either sex.
33399997	2	68	from	days	447:450	arg1	wall					402:405	cellular wall	393:405	cellular wall of gram-negative bacteria, on postnatal days 3 and 5	393:458	A double exposure of neonatal rats to lipopolysaccharide, a component of cellular wall of gram-negative bacteria, on postnatal days 3 and 5 provokes the development of depressive- and anxiety-like behaviors.
33399997	8	69	theme	GAD67-positive	1394:1407	arg1	neurons					1409:1415	calbindin-immunoreactive GAD67-positive neurons	1369:1415	calbindin-immunoreactive GAD67-positive neurons	1369:1415	The portion of calbindin-immunoreactive GAD67-positive neurons significantly increased while the portion of calretinin-immunoreactive GAD67-positive neurons significantly decreased in the CA1 field of rats exposed to NPC independently on their sex.
33399997	3	70	from	neuroplasticity	540:554	arg1	animals					579:585	adult animals	573:585	adult animals	573:585	NPC impairs neuroplasticity and cognition in adult animals, significant modifications of neuroplasticity being evident even in adolescence.
33399997	4	71	theme	neuronal	721:728	arg1	population					730:739	GABAergic neuronal population	711:739	GABAergic neuronal population of the dorsal hippocampus in juvenile male and female rats using immunofluorescent histochemistry	711:837	We studied effects of NPC on microglia and GABAergic neuronal population of the dorsal hippocampus in juvenile male and female rats using immunofluorescent histochemistry.
33399997	3	72	from	cognition	560:568	arg1	animals					579:585	adult animals	573:585	adult animals	573:585	NPC impairs neuroplasticity and cognition in adult animals, significant modifications of neuroplasticity being evident even in adolescence.
33399997	9	73	theme	neurons	1674:1680	arg1	subpopulation					1647:1659	the parvalbumin-positive subpopulation	1622:1659	the parvalbumin-positive subpopulation of GABAergic neurons in the hippocampus of rats of either sex	1622:1721	NPC did not affect the parvalbumin-positive subpopulation of GABAergic neurons in the hippocampus of rats of either sex.
33399997	4	74	theme	female	788:793	arg1	rats					795:798	juvenile male and female rats	770:798	juvenile male and female rats using immunofluorescent histochemistry	770:837	We studied effects of NPC on microglia and GABAergic neuronal population of the dorsal hippocampus in juvenile male and female rats using immunofluorescent histochemistry.
33399997	3	75	from	evident	639:645	arg1	adolescence					655:665	adolescence	655:665	adolescence	655:665	NPC impairs neuroplasticity and cognition in adult animals, significant modifications of neuroplasticity being evident even in adolescence.
33399997	0	76	theme	interneurons	116:127	arg1	subtypes					94:101	subtypes	94:101	subtypes of GABAergic interneurons in the hippocampus of juvenile rats	94:163	Neonatal proinflammatory challenge evokes a microglial response and affects the ratio between subtypes of GABAergic interneurons in the hippocampus of juvenile rats: sex-dependent and sex-independent effects.
33399997	5	77	theme	specific	1035:1042	arg1	subpopulations					1044:1057	their specific subpopulations	1029:1057	their specific subpopulations	1029:1057	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin were used as quantitative markers of GABAergic interneurons and their specific subpopulations, respectively.
33399997	6	78	theme	CA3	1174:1176	arg1	field					1178:1182	CA3 field	1174:1182	CA3 field	1174:1182	NPC induced changes of microglial morphology indicating inflammatory activation mostly expressed in CA3 field; the effect was similar in males and females.
33399997	2	79	theme	behaviors	517:525	arg1	development					473:483	the development	469:483	the development of depressive- and anxiety-like behaviors	469:525	A double exposure of neonatal rats to lipopolysaccharide, a component of cellular wall of gram-negative bacteria, on postnatal days 3 and 5 provokes the development of depressive- and anxiety-like behaviors.
33399997	2	80	theme	wall	402:405	arg1	lipopolysaccharide					358:375	lipopolysaccharide	358:375	lipopolysaccharide	358:375	A double exposure of neonatal rats to lipopolysaccharide, a component of cellular wall of gram-negative bacteria, on postnatal days 3 and 5 provokes the development of depressive- and anxiety-like behaviors.
33399997	2	80	theme	wall	402:405	arg1	component					380:388	a component	378:388	a component of cellular wall of gram-negative bacteria, on postnatal days 3 and 5	378:458	A double exposure of neonatal rats to lipopolysaccharide, a component of cellular wall of gram-negative bacteria, on postnatal days 3 and 5 provokes the development of depressive- and anxiety-like behaviors.
33399997	0	81	theme	proinflammatory	9:23	arg1	challenge					25:33	Neonatal proinflammatory challenge	0:33	Neonatal proinflammatory challenge	0:33	Neonatal proinflammatory challenge evokes a microglial response and affects the ratio between subtypes of GABAergic interneurons in the hippocampus of juvenile rats: sex-dependent and sex-independent effects.
33399997	8	82	theme	calretinin-immunoreactive	1462:1486	arg1	neurons					1503:1509	calretinin-immunoreactive GAD67-positive neurons	1462:1509	calretinin-immunoreactive GAD67-positive neurons	1462:1509	The portion of calbindin-immunoreactive GAD67-positive neurons significantly increased while the portion of calretinin-immunoreactive GAD67-positive neurons significantly decreased in the CA1 field of rats exposed to NPC independently on their sex.
33399997	6	83	from	females	1221:1227	arg1	similar					1200:1206	similar	1200:1206	similar	1200:1206	NPC induced changes of microglial morphology indicating inflammatory activation mostly expressed in CA3 field; the effect was similar in males and females.
33399997	6	83	from	females	1221:1227	arg1	effect					1189:1194	the effect	1185:1194	the effect	1185:1194	NPC induced changes of microglial morphology indicating inflammatory activation mostly expressed in CA3 field; the effect was similar in males and females.
33399997	5	84	theme	acid	867:870	arg1	markers					991:997	quantitative markers	978:997	quantitative markers of GABAergic interneurons and their specific subpopulations	978:1057	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin were used as quantitative markers of GABAergic interneurons and their specific subpopulations, respectively.
33399997	5	84	theme	acid	867:870	arg1	expression					844:853	The expression	840:853	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin	840:963	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin were used as quantitative markers of GABAergic interneurons and their specific subpopulations, respectively.
33399997	1	85	theme	disorders	299:307	arg1	development					272:282	the development	268:282	the development of psychiatric disorders in adults	268:317	Neonatal proinflammatory challenge (NPC) may contribute to the development of psychiatric disorders in adults.
33399997	5	86	theme	GABAergic	1002:1010	arg1	interneurons					1012:1023	GABAergic interneurons	1002:1023	GABAergic interneurons	1002:1023	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin were used as quantitative markers of GABAergic interneurons and their specific subpopulations, respectively.
33399997	2	87	theme	postnatal	437:445	arg1	days					447:450	postnatal days 3 and 5	437:458	days	447:450	A double exposure of neonatal rats to lipopolysaccharide, a component of cellular wall of gram-negative bacteria, on postnatal days 3 and 5 provokes the development of depressive- and anxiety-like behaviors.
33399997	4	88	theme	male	779:782	arg1	rats					795:798	juvenile male and female rats	770:798	juvenile male and female rats using immunofluorescent histochemistry	770:837	We studied effects of NPC on microglia and GABAergic neuronal population of the dorsal hippocampus in juvenile male and female rats using immunofluorescent histochemistry.
33399997	0	89	theme	microglial	44:53	arg1	response					55:62	a microglial response	42:62	a microglial response	42:62	Neonatal proinflammatory challenge evokes a microglial response and affects the ratio between subtypes of GABAergic interneurons in the hippocampus of juvenile rats: sex-dependent and sex-independent effects.
33399997	9	90	theme	sex	1719:1721	arg1	rats					1704:1707	rats	1704:1707	rats of either sex	1704:1721	NPC did not affect the parvalbumin-positive subpopulation of GABAergic neurons in the hippocampus of rats of either sex.
33399997	4	91	theme	GABAergic	711:719	arg1	population					730:739	GABAergic neuronal population	711:739	GABAergic neuronal population of the dorsal hippocampus in juvenile male and female rats using immunofluorescent histochemistry	711:837	We studied effects of NPC on microglia and GABAergic neuronal population of the dorsal hippocampus in juvenile male and female rats using immunofluorescent histochemistry.
33399997	6	92	from	similar	1200:1206	arg1	females					1221:1227	females	1221:1227	females	1221:1227	NPC induced changes of microglial morphology indicating inflammatory activation mostly expressed in CA3 field; the effect was similar in males and females.
33399997	6	92	from	similar	1200:1206	arg1	males					1211:1215	males	1211:1215	males	1211:1215	NPC induced changes of microglial morphology indicating inflammatory activation mostly expressed in CA3 field; the effect was similar in males and females.
33399997	2	93	theme	gram-negative	410:422	arg1	bacteria					424:431	gram-negative bacteria	410:431	gram-negative bacteria	410:431	A double exposure of neonatal rats to lipopolysaccharide, a component of cellular wall of gram-negative bacteria, on postnatal days 3 and 5 provokes the development of depressive- and anxiety-like behaviors.
33399997	5	94	dep	proteins	917:924	arg1	proteins					917:924	calcium-binding proteins	901:924	calcium-binding proteins calretinin, calbindin, and parvalbumin	901:963	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin were used as quantitative markers of GABAergic interneurons and their specific subpopulations, respectively.
33399997	5	94	dep	proteins	917:924	arg1	calbindin					938:946	calbindin	938:946	calbindin	938:946	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin were used as quantitative markers of GABAergic interneurons and their specific subpopulations, respectively.
33399997	5	94	dep	proteins	917:924	arg1	parvalbumin					953:963	parvalbumin	953:963	parvalbumin	953:963	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin were used as quantitative markers of GABAergic interneurons and their specific subpopulations, respectively.
33399997	5	94	dep	proteins	917:924	arg1	calretinin					926:935	calretinin	926:935	calretinin	926:935	The expression of glutamic acid decarboxylase-67 (GAD67) and calcium-binding proteins calretinin, calbindin, and parvalbumin were used as quantitative markers of GABAergic interneurons and their specific subpopulations, respectively.
33399997	6	95	from	males	1211:1215	arg1	similar					1200:1206	similar	1200:1206	similar	1200:1206	NPC induced changes of microglial morphology indicating inflammatory activation mostly expressed in CA3 field; the effect was similar in males and females.
33399997	6	95	from	males	1211:1215	arg1	effect					1189:1194	the effect	1185:1194	the effect	1185:1194	NPC induced changes of microglial morphology indicating inflammatory activation mostly expressed in CA3 field; the effect was similar in males and females.
33399997	2	96	theme	rats	350:353	arg1	exposure					329:336	A double exposure	320:336	A double exposure of neonatal rats to lipopolysaccharide, a component of cellular wall of gram-negative bacteria, on postnatal days 3 and 5	320:458	A double exposure of neonatal rats to lipopolysaccharide, a component of cellular wall of gram-negative bacteria, on postnatal days 3 and 5 provokes the development of depressive- and anxiety-like behaviors.
33399997	2	97	from	wall	402:405	arg1	days					447:450	postnatal days 3 and 5	437:458	days	447:450	A double exposure of neonatal rats to lipopolysaccharide, a component of cellular wall of gram-negative bacteria, on postnatal days 3 and 5 provokes the development of depressive- and anxiety-like behaviors.
33399997	2	97	from	wall	402:405	arg1	5					458:458	5	458:458	5	458:458	A double exposure of neonatal rats to lipopolysaccharide, a component of cellular wall of gram-negative bacteria, on postnatal days 3 and 5 provokes the development of depressive- and anxiety-like behaviors.
33399997	7	98	theme	males	1322:1326	arg1	hippocampus					1295:1305	the dorsal hippocampus	1284:1305	the dorsal hippocampus of females and males independently on the NPC	1284:1351	The number of GAD67 expressing neurons was similar in the dorsal hippocampus of females and males independently on the NPC.
33399997	10	99	theme	neuronal	1786:1793	arg1	composition					1806:1816	GABAergic neuronal population composition	1776:1816	GABAergic neuronal population composition	1776:1816	These data suggest that NPC-induced modification of GABAergic neuronal population composition under the proinflammatory conditions is involved in the maintenance of excitation/inhibition homeostatic balance in the hippocampus.
33399997	8	100	theme	rats	1555:1558	arg1	field					1546:1550	the CA1 field	1538:1550	the CA1 field of rats exposed to NPC	1538:1573	The portion of calbindin-immunoreactive GAD67-positive neurons significantly increased while the portion of calretinin-immunoreactive GAD67-positive neurons significantly decreased in the CA1 field of rats exposed to NPC independently on their sex.
33399997	7	101	theme	GAD67	1244:1248	arg1	neurons					1261:1267	GAD67 expressing neurons	1244:1267	GAD67 expressing neurons	1244:1267	The number of GAD67 expressing neurons was similar in the dorsal hippocampus of females and males independently on the NPC.
33399997	4	102	from	population	730:739	arg1	rats					795:798	juvenile male and female rats	770:798	juvenile male and female rats using immunofluorescent histochemistry	770:837	We studied effects of NPC on microglia and GABAergic neuronal population of the dorsal hippocampus in juvenile male and female rats using immunofluorescent histochemistry.
33616405	3	0	theme	minimal	796:802	arg1	components					804:813	using minimal components	790:813	using minimal components	790:813	The approach has fascinating advantages such as using minimal components, simplicity, and not requiring sacrificial template particles and toxic solvents.
33616405	0	1	with	Particles	94:102	arg1	Capability					150:159	Tunable pH-Responsive Controlled Release Capability	109:159	Tunable pH-Responsive Controlled Release Capability	109:159	Interfacial Photo-Cross-Linking: Simple but Powerful Approach for Fabricating Capsule Polymer Particles with Tunable pH-Responsive Controlled Release Capability.
33616405	1	2	theme	spherical	328:336	arg1	poly					338:341	spherical poly	328:341	spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles	328:435	Herein, we describe capsule polymer particles with precisely controlled pH-responsive release properties prepared directly via the interfacial photo-cross-linking of spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles.
33616405	1	2	theme	spherical	328:336	arg1	methacrylate					396:407	2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate	343:407	2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate	343:407	Herein, we describe capsule polymer particles with precisely controlled pH-responsive release properties prepared directly via the interfacial photo-cross-linking of spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles.
33616405	4	3	theme	encapsulated	948:959	arg1	materials					961:969	encapsulated materials	948:969	encapsulated materials	948:969	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	4	4	theme	pH	1219:1220	arg1	conditions					1222:1231	the pH conditions	1215:1231	the pH conditions	1215:1231	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	4	5	theme	release	1394:1400	arg1	kinetics					1402:1409	the release kinetics	1390:1409	the release kinetics under different pH conditions	1390:1439	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	2	6	theme	polymer	723:729	arg1	particles					731:739	spherical polymer particles	713:739	spherical polymer particles	713:739	In the interfacial photo-cross-linking, photoreactive cinnamoyl groups in the polymer particles were cross-linked via [2π + 2π] cycloaddition reactions at the polymer/water interface, showing that the shell-cross-linked hollow polymer particles can be directly prepared from spherical polymer particles.
33616405	4	7	theme	pH	1427:1428	arg1	conditions					1430:1439	different pH conditions	1417:1439	different pH conditions	1417:1439	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	2	8	theme	shell-cross-linked	639:656	arg1	particles					673:681	the shell-cross-linked hollow polymer particles	635:681	the shell-cross-linked hollow polymer particles	635:681	In the interfacial photo-cross-linking, photoreactive cinnamoyl groups in the polymer particles were cross-linked via [2π + 2π] cycloaddition reactions at the polymer/water interface, showing that the shell-cross-linked hollow polymer particles can be directly prepared from spherical polymer particles.
33616405	3	9	theme	sacrificial	846:856	arg1	particles					867:875	not requiring sacrificial template particles	832:875	not requiring sacrificial template particles	832:875	The approach has fascinating advantages such as using minimal components, simplicity, and not requiring sacrificial template particles and toxic solvents.
33616405	0	10	theme	Release	142:148	arg1	Capability					150:159	Tunable pH-Responsive Controlled Release Capability	109:159	Tunable pH-Responsive Controlled Release Capability	109:159	Interfacial Photo-Cross-Linking: Simple but Powerful Approach for Fabricating Capsule Polymer Particles with Tunable pH-Responsive Controlled Release Capability.
33616405	1	11	theme	pH-responsive	234:246	arg1	properties					256:265	precisely controlled pH-responsive release properties	213:265	precisely controlled pH-responsive release properties prepared directly via the interfacial photo-cross-linking of spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles	213:435	Herein, we describe capsule polymer particles with precisely controlled pH-responsive release properties prepared directly via the interfacial photo-cross-linking of spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles.
33616405	7	12	from	application	2052:2062	arg1	fields					2085:2090	advanced research fields	2067:2090	advanced research fields	2067:2090	With this simple interfacial photo-cross-linking strategy, we envision the ready synthesis of sophisticated particulate materials for broad application in advanced research fields.
33616405	5	13	theme	polymer	1693:1699	arg1	particles					1701:1709	spherical polymer particles	1683:1709	spherical polymer particles	1683:1709	Furthermore, by exploiting the pH-responsiveness, capsule particles are successfully obtained via an all-aqueous process from spherical polymer particles.
33616405	4	14	dep	made	938:941	arg1	retained					983:990	retained	983:990	were stably retained in the capsule particles under neutral pH conditions	971:1043	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	4	14	dep	made	938:941	arg1	II					1047:1048	II	1047:1048	II	1047:1048	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	4	14	dep	made	938:941	arg1	released					1079:1086	released	1079:1086	were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA)	1074:1554	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	4	14	dep	made	938:941	arg1	I					945:945	I	945:945	I	945:945	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	2	15	theme	polymer	665:671	arg1	particles					673:681	the shell-cross-linked hollow polymer particles	635:681	the shell-cross-linked hollow polymer particles	635:681	In the interfacial photo-cross-linking, photoreactive cinnamoyl groups in the polymer particles were cross-linked via [2π + 2π] cycloaddition reactions at the polymer/water interface, showing that the shell-cross-linked hollow polymer particles can be directly prepared from spherical polymer particles.
33616405	3	16	theme	toxic	881:885	arg1	particles					867:875	not requiring sacrificial template particles	832:875	not requiring sacrificial template particles	832:875	The approach has fascinating advantages such as using minimal components, simplicity, and not requiring sacrificial template particles and toxic solvents.
33616405	3	16	theme	toxic	881:885	arg1	solvents					887:894	toxic solvents	881:894	toxic solvents	881:894	The approach has fascinating advantages such as using minimal components, simplicity, and not requiring sacrificial template particles and toxic solvents.
33616405	3	16	theme	toxic	881:885	arg1	simplicity					816:825	simplicity	816:825	simplicity	816:825	The approach has fascinating advantages such as using minimal components, simplicity, and not requiring sacrificial template particles and toxic solvents.
33616405	3	16	theme	toxic	881:885	arg1	components					804:813	using minimal components	790:813	using minimal components	790:813	The approach has fascinating advantages such as using minimal components, simplicity, and not requiring sacrificial template particles and toxic solvents.
33616405	4	17	dep	controlled	1201:1210	arg1	III					1144:1146	III	1144:1146	III	1144:1146	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	4	18	theme	neutral	1023:1029	arg1	conditions					1034:1043	neutral pH conditions	1023:1043	neutral pH conditions	1023:1043	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	6	19	theme	process	1760:1766	arg1	advantages					1716:1725	The advantages	1712:1725	The advantages of the all-aqueous encapsulation process	1712:1766	The advantages of the all-aqueous encapsulation process allowed the water-soluble biomacromolecules such as DNA and saccharides to be successfully encapsulated in the P(DEAEMA-CEMA) hollow particles.
33616405	6	20	theme	all-aqueous	1734:1744	arg1	process					1760:1766	the all-aqueous encapsulation process	1730:1766	the all-aqueous encapsulation process	1730:1766	The advantages of the all-aqueous encapsulation process allowed the water-soluble biomacromolecules such as DNA and saccharides to be successfully encapsulated in the P(DEAEMA-CEMA) hollow particles.
33616405	4	21	theme	polymer	1518:1524	arg1	composition					1526:1536	the polymer composition	1514:1536	the polymer composition in P(DEAEMA-CEMA)	1514:1554	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	2	22	theme	cycloaddition	566:578	arg1	reactions					580:588	[2π + 2π] cycloaddition reactions	556:588	[2π + 2π] cycloaddition reactions	556:588	In the interfacial photo-cross-linking, photoreactive cinnamoyl groups in the polymer particles were cross-linked via [2π + 2π] cycloaddition reactions at the polymer/water interface, showing that the shell-cross-linked hollow polymer particles can be directly prepared from spherical polymer particles.
33616405	0	23	theme	Polymer	86:92	arg1	Particles					94:102	Capsule Polymer Particles	78:102	Capsule Polymer Particles with Tunable pH-Responsive Controlled Release Capability	78:159	Interfacial Photo-Cross-Linking: Simple but Powerful Approach for Fabricating Capsule Polymer Particles with Tunable pH-Responsive Controlled Release Capability.
33616405	1	24	theme	capsule	182:188	arg1	particles					198:206	capsule polymer particles	182:206	capsule polymer particles with precisely controlled pH-responsive release properties prepared directly via the interfacial photo-cross-linking of spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles	182:435	Herein, we describe capsule polymer particles with precisely controlled pH-responsive release properties prepared directly via the interfacial photo-cross-linking of spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles.
33616405	1	25	theme	particles	427:435	arg1	photo-cross-linking					305:323	the interfacial photo-cross-linking	289:323	the interfacial photo-cross-linking of spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles	289:435	Herein, we describe capsule polymer particles with precisely controlled pH-responsive release properties prepared directly via the interfacial photo-cross-linking of spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles.
33616405	7	26	theme	advanced	2067:2074	arg1	fields					2085:2090	advanced research fields	2067:2090	advanced research fields	2067:2090	With this simple interfacial photo-cross-linking strategy, we envision the ready synthesis of sophisticated particulate materials for broad application in advanced research fields.
33616405	2	27	theme	polymer/water	597:609	arg1	interface					611:619	the polymer/water interface	593:619	the polymer/water interface	593:619	In the interfacial photo-cross-linking, photoreactive cinnamoyl groups in the polymer particles were cross-linked via [2π + 2π] cycloaddition reactions at the polymer/water interface, showing that the shell-cross-linked hollow polymer particles can be directly prepared from spherical polymer particles.
33616405	4	28	theme	media	1323:1327	arg1	acidity					1300:1306	the acidity	1296:1306	the acidity of the aqueous media	1296:1327	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	2	29	theme	photoreactive	478:490	arg1	groups					502:507	photoreactive cinnamoyl groups	478:507	photoreactive cinnamoyl groups in the polymer particles	478:532	In the interfacial photo-cross-linking, photoreactive cinnamoyl groups in the polymer particles were cross-linked via [2π + 2π] cycloaddition reactions at the polymer/water interface, showing that the shell-cross-linked hollow polymer particles can be directly prepared from spherical polymer particles.
33616405	0	30	theme	pH-Responsive	117:129	arg1	Capability					150:159	Tunable pH-Responsive Controlled Release Capability	109:159	Tunable pH-Responsive Controlled Release Capability	109:159	Interfacial Photo-Cross-Linking: Simple but Powerful Approach for Fabricating Capsule Polymer Particles with Tunable pH-Responsive Controlled Release Capability.
33616405	0	31	theme	Interfacial	0:10	arg1	Photo-Cross-Linking					12:30	Interfacial Photo-Cross-Linking	0:30	Interfacial Photo-Cross-Linking: Simple but Powerful Approach for Fabricating Capsule Polymer Particles with Tunable pH-Responsive Controlled Release Capability.	0:160	Interfacial Photo-Cross-Linking: Simple but Powerful Approach for Fabricating Capsule Polymer Particles with Tunable pH-Responsive Controlled Release Capability.
33616405	1	32	theme	poly	338:341	arg1	particles					427:435	spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles	328:435	spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles	328:435	Herein, we describe capsule polymer particles with precisely controlled pH-responsive release properties prepared directly via the interfacial photo-cross-linking of spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles.
33616405	4	33	theme	photoirradiation	1339:1354	arg1	time					1356:1359	the photoirradiation time	1335:1359	the photoirradiation time	1335:1359	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	6	34	theme	hollow	1894:1899	arg1	particles					1901:1909	the P(DEAEMA-CEMA) hollow particles	1875:1909	the P(DEAEMA-CEMA) hollow particles	1875:1909	The advantages of the all-aqueous encapsulation process allowed the water-soluble biomacromolecules such as DNA and saccharides to be successfully encapsulated in the P(DEAEMA-CEMA) hollow particles.
33616405	1	35	theme	2-diethylaminoethyl	343:361	arg1	poly					338:341	spherical poly	328:341	spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles	328:435	Herein, we describe capsule polymer particles with precisely controlled pH-responsive release properties prepared directly via the interfacial photo-cross-linking of spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles.
33616405	1	35	theme	2-diethylaminoethyl	343:361	arg1	methacrylate					396:407	2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate	343:407	2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate	343:407	Herein, we describe capsule polymer particles with precisely controlled pH-responsive release properties prepared directly via the interfacial photo-cross-linking of spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles.
33616405	7	36	theme	particulate	2020:2030	arg1	materials					2032:2040	sophisticated particulate materials	2006:2040	sophisticated particulate materials	2006:2040	With this simple interfacial photo-cross-linking strategy, we envision the ready synthesis of sophisticated particulate materials for broad application in advanced research fields.
33616405	2	37	theme	2π	562:563	arg1	reactions					580:588	[2π + 2π] cycloaddition reactions	556:588	[2π + 2π] cycloaddition reactions	556:588	In the interfacial photo-cross-linking, photoreactive cinnamoyl groups in the polymer particles were cross-linked via [2π + 2π] cycloaddition reactions at the polymer/water interface, showing that the shell-cross-linked hollow polymer particles can be directly prepared from spherical polymer particles.
33616405	5	38	theme	capsule	1607:1613	arg1	particles					1615:1623	capsule particles	1607:1623	capsule particles	1607:1623	Furthermore, by exploiting the pH-responsiveness, capsule particles are successfully obtained via an all-aqueous process from spherical polymer particles.
33616405	3	39	theme	fascinating	759:769	arg1	particles					867:875	not requiring sacrificial template particles	832:875	not requiring sacrificial template particles	832:875	The approach has fascinating advantages such as using minimal components, simplicity, and not requiring sacrificial template particles and toxic solvents.
33616405	3	39	theme	fascinating	759:769	arg1	simplicity					816:825	simplicity	816:825	simplicity	816:825	The approach has fascinating advantages such as using minimal components, simplicity, and not requiring sacrificial template particles and toxic solvents.
33616405	3	39	theme	fascinating	759:769	arg1	components					804:813	using minimal components	790:813	using minimal components	790:813	The approach has fascinating advantages such as using minimal components, simplicity, and not requiring sacrificial template particles and toxic solvents.
33616405	3	39	theme	fascinating	759:769	arg1	advantages					771:780	fascinating advantages	759:780	fascinating advantages such as using minimal components, simplicity, and not requiring sacrificial template particles	759:875	The approach has fascinating advantages such as using minimal components, simplicity, and not requiring sacrificial template particles and toxic solvents.
33616405	4	40	from	composition	1526:1536	arg1	P					1541:1541	P	1541:1541	P(DEAEMA-CEMA)	1541:1554	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	4	40	from	composition	1526:1536	arg1	DEAEMA-CEMA					1543:1553	DEAEMA-CEMA	1543:1553	DEAEMA-CEMA	1543:1553	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	2	41	theme	[2π	556:558	arg1	reactions					580:588	[2π + 2π] cycloaddition reactions	556:588	[2π + 2π] cycloaddition reactions	556:588	In the interfacial photo-cross-linking, photoreactive cinnamoyl groups in the polymer particles were cross-linked via [2π + 2π] cycloaddition reactions at the polymer/water interface, showing that the shell-cross-linked hollow polymer particles can be directly prepared from spherical polymer particles.
33616405	6	42	theme	P	1879:1879	arg1	particles					1901:1909	the P(DEAEMA-CEMA) hollow particles	1875:1909	the P(DEAEMA-CEMA) hollow particles	1875:1909	The advantages of the all-aqueous encapsulation process allowed the water-soluble biomacromolecules such as DNA and saccharides to be successfully encapsulated in the P(DEAEMA-CEMA) hollow particles.
33616405	7	43	theme	ready	1987:1991	arg1	synthesis					1993:2001	the ready synthesis	1983:2001	the ready synthesis of sophisticated particulate materials for broad application in advanced research fields	1983:2090	With this simple interfacial photo-cross-linking strategy, we envision the ready synthesis of sophisticated particulate materials for broad application in advanced research fields.
33616405	7	44	theme	interfacial	1929:1939	arg1	strategy					1961:1968	this simple interfacial photo-cross-linking strategy	1917:1968	this simple interfacial photo-cross-linking strategy	1917:1968	With this simple interfacial photo-cross-linking strategy, we envision the ready synthesis of sophisticated particulate materials for broad application in advanced research fields.
33616405	3	45	theme	using	790:794	arg1	components					804:813	using minimal components	790:813	using minimal components	790:813	The approach has fascinating advantages such as using minimal components, simplicity, and not requiring sacrificial template particles and toxic solvents.
33616405	4	46	theme	important	911:919	arg1	observations					921:932	The following important observations	897:932	The following important observations	897:932	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	1	47	with	particles	198:206	arg1	properties					256:265	precisely controlled pH-responsive release properties	213:265	precisely controlled pH-responsive release properties prepared directly via the interfacial photo-cross-linking of spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles	213:435	Herein, we describe capsule polymer particles with precisely controlled pH-responsive release properties prepared directly via the interfacial photo-cross-linking of spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles.
33616405	2	48	from	groups	502:507	arg1	particles					524:532	the polymer particles	512:532	the polymer particles	512:532	In the interfacial photo-cross-linking, photoreactive cinnamoyl groups in the polymer particles were cross-linked via [2π + 2π] cycloaddition reactions at the polymer/water interface, showing that the shell-cross-linked hollow polymer particles can be directly prepared from spherical polymer particles.
33616405	4	49	theme	materials	1186:1194	arg1	kinetics					1161:1168	the release kinetics	1149:1168	the release kinetics of encapsulated materials	1149:1194	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	4	50	theme	acidic	1121:1126	arg1	conditions					1131:1140	acidic pH conditions	1121:1140	acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA)	1121:1554	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	5	51	from	particles	1701:1709	arg1	process					1670:1676	an all-aqueous process	1655:1676	an all-aqueous process from spherical polymer particles	1655:1709	Furthermore, by exploiting the pH-responsiveness, capsule particles are successfully obtained via an all-aqueous process from spherical polymer particles.
33616405	1	52	theme	interfacial	293:303	arg1	photo-cross-linking					305:323	the interfacial photo-cross-linking	289:323	the interfacial photo-cross-linking of spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles	289:435	Herein, we describe capsule polymer particles with precisely controlled pH-responsive release properties prepared directly via the interfacial photo-cross-linking of spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles.
33616405	4	53	theme	release	1153:1159	arg1	kinetics					1161:1168	the release kinetics	1149:1168	the release kinetics of encapsulated materials	1149:1194	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	4	54	theme	different	1417:1425	arg1	conditions					1430:1439	different pH conditions	1417:1439	different pH conditions	1417:1439	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	3	55	theme	requiring	836:844	arg1	particles					867:875	not requiring sacrificial template particles	832:875	not requiring sacrificial template particles	832:875	The approach has fascinating advantages such as using minimal components, simplicity, and not requiring sacrificial template particles and toxic solvents.
33616405	1	56	theme	polymer	190:196	arg1	particles					198:206	capsule polymer particles	182:206	capsule polymer particles with precisely controlled pH-responsive release properties prepared directly via the interfacial photo-cross-linking of spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles	182:435	Herein, we describe capsule polymer particles with precisely controlled pH-responsive release properties prepared directly via the interfacial photo-cross-linking of spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles.
33616405	2	57	link	shell-cross-linked	639:656	arg1	particles					673:681	the shell-cross-linked hollow polymer particles	635:681	the shell-cross-linked hollow polymer particles	635:681	In the interfacial photo-cross-linking, photoreactive cinnamoyl groups in the polymer particles were cross-linked via [2π + 2π] cycloaddition reactions at the polymer/water interface, showing that the shell-cross-linked hollow polymer particles can be directly prepared from spherical polymer particles.
33616405	4	58	dep	conditions	1131:1140	arg1	controlled					1201:1210	controlled	1201:1210	were controlled by the pH conditions	1196:1231	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	2	59	theme	spherical	713:721	arg1	particles					731:739	spherical polymer particles	713:739	spherical polymer particles	713:739	In the interfacial photo-cross-linking, photoreactive cinnamoyl groups in the polymer particles were cross-linked via [2π + 2π] cycloaddition reactions at the polymer/water interface, showing that the shell-cross-linked hollow polymer particles can be directly prepared from spherical polymer particles.
33616405	1	60	theme	controlled	223:232	arg1	properties					256:265	precisely controlled pH-responsive release properties	213:265	precisely controlled pH-responsive release properties prepared directly via the interfacial photo-cross-linking of spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles	213:435	Herein, we describe capsule polymer particles with precisely controlled pH-responsive release properties prepared directly via the interfacial photo-cross-linking of spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles.
33616405	4	61	dep	affect	1383:1388	arg1	regulated					1492:1500	regulated	1492:1500	were regulated by changing the polymer composition in P(DEAEMA-CEMA)	1487:1554	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	4	61	dep	affect	1383:1388	arg1	IV					1331:1332	IV	1331:1332	IV	1331:1332	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	4	62	theme	pH	1031:1032	arg1	conditions					1034:1043	neutral pH conditions	1023:1043	neutral pH conditions	1023:1043	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	5	63	theme	spherical	1683:1691	arg1	particles					1701:1709	spherical polymer particles	1683:1709	spherical polymer particles	1683:1709	Furthermore, by exploiting the pH-responsiveness, capsule particles are successfully obtained via an all-aqueous process from spherical polymer particles.
33616405	3	64	theme	template	858:865	arg1	particles					867:875	not requiring sacrificial template particles	832:875	not requiring sacrificial template particles	832:875	The approach has fascinating advantages such as using minimal components, simplicity, and not requiring sacrificial template particles and toxic solvents.
33616405	1	65	theme	release	248:254	arg1	properties					256:265	precisely controlled pH-responsive release properties	213:265	precisely controlled pH-responsive release properties prepared directly via the interfacial photo-cross-linking of spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles	213:435	Herein, we describe capsule polymer particles with precisely controlled pH-responsive release properties prepared directly via the interfacial photo-cross-linking of spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles.
33616405	6	66	theme	water-soluble	1780:1792	arg1	saccharides					1828:1838	saccharides	1828:1838	saccharides	1828:1838	The advantages of the all-aqueous encapsulation process allowed the water-soluble biomacromolecules such as DNA and saccharides to be successfully encapsulated in the P(DEAEMA-CEMA) hollow particles.
33616405	6	66	theme	water-soluble	1780:1792	arg1	biomacromolecules					1794:1810	the water-soluble biomacromolecules	1776:1810	the water-soluble biomacromolecules such as DNA and saccharides	1776:1838	The advantages of the all-aqueous encapsulation process allowed the water-soluble biomacromolecules such as DNA and saccharides to be successfully encapsulated in the P(DEAEMA-CEMA) hollow particles.
33616405	6	66	theme	water-soluble	1780:1792	arg1	DNA					1820:1822	DNA	1820:1822	DNA	1820:1822	The advantages of the all-aqueous encapsulation process allowed the water-soluble biomacromolecules such as DNA and saccharides to be successfully encapsulated in the P(DEAEMA-CEMA) hollow particles.
33616405	3	67	contain	has	755:757	arg2	simplicity					816:825	simplicity	816:825	simplicity	816:825	The approach has fascinating advantages such as using minimal components, simplicity, and not requiring sacrificial template particles and toxic solvents.
33616405	3	67	contain	has	755:757	arg1	approach					746:753	The approach	742:753	The approach	742:753	The approach has fascinating advantages such as using minimal components, simplicity, and not requiring sacrificial template particles and toxic solvents.
33616405	3	67	contain	has	755:757	arg2	particles					867:875	not requiring sacrificial template particles	832:875	not requiring sacrificial template particles	832:875	The approach has fascinating advantages such as using minimal components, simplicity, and not requiring sacrificial template particles and toxic solvents.
33616405	3	67	contain	has	755:757	arg2	solvents					887:894	toxic solvents	881:894	toxic solvents	881:894	The approach has fascinating advantages such as using minimal components, simplicity, and not requiring sacrificial template particles and toxic solvents.
33616405	3	67	contain	has	755:757	arg2	components					804:813	using minimal components	790:813	using minimal components	790:813	The approach has fascinating advantages such as using minimal components, simplicity, and not requiring sacrificial template particles and toxic solvents.
33616405	3	67	contain	has	755:757	arg2	advantages					771:780	fascinating advantages	759:780	fascinating advantages such as using minimal components, simplicity, and not requiring sacrificial template particles	759:875	The approach has fascinating advantages such as using minimal components, simplicity, and not requiring sacrificial template particles and toxic solvents.
33616405	4	68	theme	capsule	999:1005	arg1	particles					1007:1015	the capsule particles	995:1015	the capsule particles	995:1015	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	2	69	theme	hollow	658:663	arg1	particles					673:681	the shell-cross-linked hollow polymer particles	635:681	the shell-cross-linked hollow polymer particles	635:681	In the interfacial photo-cross-linking, photoreactive cinnamoyl groups in the polymer particles were cross-linked via [2π + 2π] cycloaddition reactions at the polymer/water interface, showing that the shell-cross-linked hollow polymer particles can be directly prepared from spherical polymer particles.
33616405	6	70	theme	encapsulation	1746:1758	arg1	process					1760:1766	the all-aqueous encapsulation process	1730:1766	the all-aqueous encapsulation process	1730:1766	The advantages of the all-aqueous encapsulation process allowed the water-soluble biomacromolecules such as DNA and saccharides to be successfully encapsulated in the P(DEAEMA-CEMA) hollow particles.
33616405	4	71	theme	immediate	1240:1248	arg1	release					1264:1270	immediate and sustained release	1240:1270	immediate and sustained release	1240:1270	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	0	72	theme	Capsule	78:84	arg1	Particles					94:102	Capsule Polymer Particles	78:102	Capsule Polymer Particles with Tunable pH-Responsive Controlled Release Capability	78:159	Interfacial Photo-Cross-Linking: Simple but Powerful Approach for Fabricating Capsule Polymer Particles with Tunable pH-Responsive Controlled Release Capability.
33616405	4	73	theme	sustained	1254:1262	arg1	release					1264:1270	immediate and sustained release	1240:1270	immediate and sustained release	1240:1270	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	4	74	theme	pH-responsive	1454:1466	arg1	properties					1476:1485	the pH-responsive release properties	1450:1485	the pH-responsive release properties	1450:1485	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	7	75	theme	research	2076:2083	arg1	fields					2085:2090	advanced research fields	2067:2090	advanced research fields	2067:2090	With this simple interfacial photo-cross-linking strategy, we envision the ready synthesis of sophisticated particulate materials for broad application in advanced research fields.
33616405	1	76	dep	poly	338:341	arg1	DEAEMA-CEMA					413:423	DEAEMA-CEMA	413:423	DEAEMA-CEMA	413:423	Herein, we describe capsule polymer particles with precisely controlled pH-responsive release properties prepared directly via the interfacial photo-cross-linking of spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles.
33616405	1	76	dep	poly	338:341	arg1	P					411:411	P	411:411	P(DEAEMA-CEMA)	411:424	Herein, we describe capsule polymer particles with precisely controlled pH-responsive release properties prepared directly via the interfacial photo-cross-linking of spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles.
33616405	4	77	theme	aqueous	1315:1321	arg1	media					1323:1327	the aqueous media	1311:1327	the aqueous media	1311:1327	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	4	78	theme	release	1468:1474	arg1	properties					1476:1485	the pH-responsive release properties	1450:1485	the pH-responsive release properties	1450:1485	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	0	79	theme	Tunable	109:115	arg1	Capability					150:159	Tunable pH-Responsive Controlled Release Capability	109:159	Tunable pH-Responsive Controlled Release Capability	109:159	Interfacial Photo-Cross-Linking: Simple but Powerful Approach for Fabricating Capsule Polymer Particles with Tunable pH-Responsive Controlled Release Capability.
33616405	0	80	theme	Controlled	131:140	arg1	Capability					150:159	Tunable pH-Responsive Controlled Release Capability	109:159	Tunable pH-Responsive Controlled Release Capability	109:159	Interfacial Photo-Cross-Linking: Simple but Powerful Approach for Fabricating Capsule Polymer Particles with Tunable pH-Responsive Controlled Release Capability.
33616405	7	81	theme	broad	2046:2050	arg1	application					2052:2062	broad application	2046:2062	broad application in advanced research fields	2046:2090	With this simple interfacial photo-cross-linking strategy, we envision the ready synthesis of sophisticated particulate materials for broad application in advanced research fields.
33616405	4	82	theme	encapsulated	1051:1062	arg1	materials					1064:1072	encapsulated materials	1051:1072	encapsulated materials	1051:1072	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	7	83	theme	materials	2032:2040	arg1	synthesis					1993:2001	the ready synthesis	1983:2001	the ready synthesis of sophisticated particulate materials for broad application in advanced research fields	1983:2090	With this simple interfacial photo-cross-linking strategy, we envision the ready synthesis of sophisticated particulate materials for broad application in advanced research fields.
33616405	2	84	attach	cross-linked	539:550	arg3	reactions					580:588	[2π + 2π] cycloaddition reactions	556:588	[2π + 2π] cycloaddition reactions	556:588	In the interfacial photo-cross-linking, photoreactive cinnamoyl groups in the polymer particles were cross-linked via [2π + 2π] cycloaddition reactions at the polymer/water interface, showing that the shell-cross-linked hollow polymer particles can be directly prepared from spherical polymer particles.
33616405	2	84	attach	cross-linked	539:550	arg3	photo-cross-linking					457:475	the interfacial photo-cross-linking	441:475	the interfacial photo-cross-linking	441:475	In the interfacial photo-cross-linking, photoreactive cinnamoyl groups in the polymer particles were cross-linked via [2π + 2π] cycloaddition reactions at the polymer/water interface, showing that the shell-cross-linked hollow polymer particles can be directly prepared from spherical polymer particles.
33616405	2	84	attach	cross-linked	539:550	arg3	interface					611:619	the polymer/water interface	593:619	the polymer/water interface	593:619	In the interfacial photo-cross-linking, photoreactive cinnamoyl groups in the polymer particles were cross-linked via [2π + 2π] cycloaddition reactions at the polymer/water interface, showing that the shell-cross-linked hollow polymer particles can be directly prepared from spherical polymer particles.
33616405	4	85	theme	pH	1128:1129	arg1	conditions					1131:1140	acidic pH conditions	1121:1140	acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA)	1121:1554	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	2	86	theme	interfacial	445:455	arg1	photo-cross-linking					457:475	the interfacial photo-cross-linking	441:475	the interfacial photo-cross-linking	441:475	In the interfacial photo-cross-linking, photoreactive cinnamoyl groups in the polymer particles were cross-linked via [2π + 2π] cycloaddition reactions at the polymer/water interface, showing that the shell-cross-linked hollow polymer particles can be directly prepared from spherical polymer particles.
33616405	0	87	theme	Simple	33:38	arg1	Approach					53:60	Simple but Powerful Approach	33:60	Interfacial Photo-Cross-Linking: Simple but Powerful Approach for Fabricating Capsule Polymer Particles with Tunable pH-Responsive Controlled Release Capability.	0:160	Interfacial Photo-Cross-Linking: Simple but Powerful Approach for Fabricating Capsule Polymer Particles with Tunable pH-Responsive Controlled Release Capability.
33616405	7	88	theme	sophisticated	2006:2018	arg1	materials					2032:2040	sophisticated particulate materials	2006:2040	sophisticated particulate materials	2006:2040	With this simple interfacial photo-cross-linking strategy, we envision the ready synthesis of sophisticated particulate materials for broad application in advanced research fields.
33616405	2	89	theme	polymer	516:522	arg1	particles					524:532	the polymer particles	512:532	the polymer particles	512:532	In the interfacial photo-cross-linking, photoreactive cinnamoyl groups in the polymer particles were cross-linked via [2π + 2π] cycloaddition reactions at the polymer/water interface, showing that the shell-cross-linked hollow polymer particles can be directly prepared from spherical polymer particles.
33616405	0	90	theme	Powerful	44:51	arg1	Approach					53:60	Simple but Powerful Approach	33:60	Interfacial Photo-Cross-Linking: Simple but Powerful Approach for Fabricating Capsule Polymer Particles with Tunable pH-Responsive Controlled Release Capability.	0:160	Interfacial Photo-Cross-Linking: Simple but Powerful Approach for Fabricating Capsule Polymer Particles with Tunable pH-Responsive Controlled Release Capability.
33616405	1	91	theme	methacrylate-co-2-cinnamoylethyl	363:394	arg1	poly					338:341	spherical poly	328:341	spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles	328:435	Herein, we describe capsule polymer particles with precisely controlled pH-responsive release properties prepared directly via the interfacial photo-cross-linking of spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles.
33616405	1	91	theme	methacrylate-co-2-cinnamoylethyl	363:394	arg1	methacrylate					396:407	2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate	343:407	2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate	343:407	Herein, we describe capsule polymer particles with precisely controlled pH-responsive release properties prepared directly via the interfacial photo-cross-linking of spherical poly(2-diethylaminoethyl methacrylate-co-2-cinnamoylethyl methacrylate) (P(DEAEMA-CEMA)) particles.
33616405	4	92	theme	capsule	1097:1103	arg1	particles					1105:1113	the capsule particles	1093:1113	the capsule particles	1093:1113	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	2	93	theme	cinnamoyl	492:500	arg1	groups					502:507	photoreactive cinnamoyl groups	478:507	photoreactive cinnamoyl groups in the polymer particles	478:532	In the interfacial photo-cross-linking, photoreactive cinnamoyl groups in the polymer particles were cross-linked via [2π + 2π] cycloaddition reactions at the polymer/water interface, showing that the shell-cross-linked hollow polymer particles can be directly prepared from spherical polymer particles.
33616405	4	94	attach	released	1079:1086	arg2	materials					1064:1072	encapsulated materials	1051:1072	encapsulated materials	1051:1072	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	4	94	attach	released	1079:1086	arg1	particles					1105:1113	the capsule particles	1093:1113	the capsule particles	1093:1113	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	4	94	attach	released	1079:1086	arg2	observations					921:932	The following important observations	897:932	The following important observations	897:932	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	7	95	theme	photo-cross-linking	1941:1959	arg1	strategy					1961:1968	this simple interfacial photo-cross-linking strategy	1917:1968	this simple interfacial photo-cross-linking strategy	1917:1968	With this simple interfacial photo-cross-linking strategy, we envision the ready synthesis of sophisticated particulate materials for broad application in advanced research fields.
33616405	4	96	theme	following	901:909	arg1	observations					921:932	The following important observations	897:932	The following important observations	897:932	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	0	97	dep	Photo-Cross-Linking	12:30	arg1	Approach					53:60	Simple but Powerful Approach	33:60	Interfacial Photo-Cross-Linking: Simple but Powerful Approach for Fabricating Capsule Polymer Particles with Tunable pH-Responsive Controlled Release Capability.	0:160	Interfacial Photo-Cross-Linking: Simple but Powerful Approach for Fabricating Capsule Polymer Particles with Tunable pH-Responsive Controlled Release Capability.
33616405	7	98	theme	simple	1922:1927	arg1	strategy					1961:1968	this simple interfacial photo-cross-linking strategy	1917:1968	this simple interfacial photo-cross-linking strategy	1917:1968	With this simple interfacial photo-cross-linking strategy, we envision the ready synthesis of sophisticated particulate materials for broad application in advanced research fields.
33616405	4	99	theme	encapsulated	1173:1184	arg1	materials					1186:1194	encapsulated materials	1173:1194	encapsulated materials	1173:1194	The following important observations are made: (I) encapsulated materials were stably retained in the capsule particles under neutral pH conditions; (II) encapsulated materials were released from the capsule particles under acidic pH conditions; (III) the release kinetics of encapsulated materials were controlled by the pH conditions; i.e., immediate and sustained release was achieved by varying the acidity of the aqueous media; (IV) the photoirradiation time did not significantly affect the release kinetics under different pH conditions; and (V) the pH-responsive release properties were regulated by changing the polymer composition in P(DEAEMA-CEMA).
33616405	5	100	theme	all-aqueous	1658:1668	arg1	process					1670:1676	an all-aqueous process	1655:1676	an all-aqueous process from spherical polymer particles	1655:1709	Furthermore, by exploiting the pH-responsiveness, capsule particles are successfully obtained via an all-aqueous process from spherical polymer particles.
32316544	5	0	theme	intestinal	773:782	arg1	inflammation					784:795	intestinal inflammation	773:795	intestinal inflammation	773:795	Ex vivo, intestinal inflammation, fecal bacterial load and composition were studied by immunohistochemistry analyses, quantitative PCR, 16 S RNA PCR or next generation sequencing in samples collected from analyzed mice.
32316544	7	1	theme	microbiome	1134:1143	arg1	composition					1145:1155	altered microbiome composition	1126:1155	altered microbiome composition	1126:1155	In vivo, oral saccharin intake reduced fecal bacterial load and altered microbiome composition, while the intestinal barrier was not obviously affected.
32316544	2	2	theme	intestinal	400:409	arg1	inflammation					411:422	intestinal inflammation	400:422	intestinal inflammation	400:422	Since the artificial sweetener saccharin is known to depict bacteriostatic and microbiome-modulating properties, we hypothesized oral saccharin intake to influence intestinal inflammation and aimed at delineating its effect on acute and chronic colitis activity in mice.
32316544	6	3	theme	bacterial	1014:1022	arg1	growth					1024:1029	bacterial growth	1014:1029	bacterial growth	1014:1029	In vitro, saccharin inhibited bacterial growth in a species-dependent manner.
32316544	9	4	theme	chronic	1500:1506	arg1	activity					1516:1523	acute and chronic colitis activity	1490:1523	acute and chronic colitis activity in mice	1490:1531	Together, this study demonstrates that oral saccharin intake decreases intestinal bacteria count and hence encompasses the capacity to reduce acute and chronic colitis activity in mice.
32316544	1	5	theme	Western	175:181	arg1	"					187:187	the "Western diet"	170:187	the "Western diet"	170:187	Non-caloric artificial sweeteners are frequently discussed as components of the "Western diet", negatively modulating intestinal homeostasis.
32316544	2	6	from	activity	489:496	arg1	mice					501:504	mice	501:504	mice	501:504	Since the artificial sweetener saccharin is known to depict bacteriostatic and microbiome-modulating properties, we hypothesized oral saccharin intake to influence intestinal inflammation and aimed at delineating its effect on acute and chronic colitis activity in mice.
32316544	2	7	theme	microbiome-modulating	315:335	arg1	properties					337:346	bacteriostatic and microbiome-modulating properties	296:346	bacteriostatic and microbiome-modulating properties	296:346	Since the artificial sweetener saccharin is known to depict bacteriostatic and microbiome-modulating properties, we hypothesized oral saccharin intake to influence intestinal inflammation and aimed at delineating its effect on acute and chronic colitis activity in mice.
32316544	4	8	theme	acute	664:668	arg1	colitis					681:687	acute or chronic colitis	664:687	acute or chronic colitis using dextran sodium sulfate (DSS)	664:722	Mice were supplemented with saccharin before or after induction of acute or chronic colitis using dextran sodium sulfate (DSS) and the extent of colitis was assessed.
32316544	7	9	theme	altered	1126:1132	arg1	composition					1145:1155	altered microbiome composition	1126:1155	altered microbiome composition	1126:1155	In vivo, oral saccharin intake reduced fecal bacterial load and altered microbiome composition, while the intestinal barrier was not obviously affected.
32316544	3	10	theme	different	517:525	arg1	strains					537:543	different bacterial strains	517:543	different bacterial strains	517:543	In vitro, different bacterial strains were grown in the presence or absence of saccharin.
32316544	3	11	dep	presence	563:570	arg1	the					559:561	the	559:561	the	559:561	In vitro, different bacterial strains were grown in the presence or absence of saccharin.
32316544	5	12	theme	immunohistochemistry	851:870	arg1	analyses					872:879	immunohistochemistry analyses	851:879	immunohistochemistry analyses	851:879	Ex vivo, intestinal inflammation, fecal bacterial load and composition were studied by immunohistochemistry analyses, quantitative PCR, 16 S RNA PCR or next generation sequencing in samples collected from analyzed mice.
32316544	5	12	theme	immunohistochemistry	851:870	arg1	PCR					895:897	quantitative PCR	882:897	quantitative PCR	882:897	Ex vivo, intestinal inflammation, fecal bacterial load and composition were studied by immunohistochemistry analyses, quantitative PCR, 16 S RNA PCR or next generation sequencing in samples collected from analyzed mice.
32316544	5	12	theme	immunohistochemistry	851:870	arg1	PCR					909:911	16 S RNA PCR	900:911	16 S RNA PCR	900:911	Ex vivo, intestinal inflammation, fecal bacterial load and composition were studied by immunohistochemistry analyses, quantitative PCR, 16 S RNA PCR or next generation sequencing in samples collected from analyzed mice.
32316544	5	12	theme	immunohistochemistry	851:870	arg1	sequencing					932:941	next generation sequencing	916:941	next generation sequencing	916:941	Ex vivo, intestinal inflammation, fecal bacterial load and composition were studied by immunohistochemistry analyses, quantitative PCR, 16 S RNA PCR or next generation sequencing in samples collected from analyzed mice.
32316544	2	13	theme	acute	463:467	arg1	activity					489:496	acute and chronic colitis activity	463:496	acute and chronic colitis activity in mice	463:504	Since the artificial sweetener saccharin is known to depict bacteriostatic and microbiome-modulating properties, we hypothesized oral saccharin intake to influence intestinal inflammation and aimed at delineating its effect on acute and chronic colitis activity in mice.
32316544	2	14	theme	bacteriostatic	296:309	arg1	properties					337:346	bacteriostatic and microbiome-modulating properties	296:346	bacteriostatic and microbiome-modulating properties	296:346	Since the artificial sweetener saccharin is known to depict bacteriostatic and microbiome-modulating properties, we hypothesized oral saccharin intake to influence intestinal inflammation and aimed at delineating its effect on acute and chronic colitis activity in mice.
32316544	1	15	theme	diet	183:186	arg1	"					187:187	the "Western diet"	170:187	the "Western diet"	170:187	Non-caloric artificial sweeteners are frequently discussed as components of the "Western diet", negatively modulating intestinal homeostasis.
32316544	8	16	theme	colitis	1236:1242	arg1	activity					1244:1251	DSS-induced colitis activity	1224:1251	DSS-induced colitis activity	1224:1251	Of note, DSS-induced colitis activity was significantly improved in mice after therapeutic or prophylactic treatment with saccharin.
32316544	1	17	theme	"	187:187	arg1	components					156:165	components	156:165	components of the "Western diet"	156:187	Non-caloric artificial sweeteners are frequently discussed as components of the "Western diet", negatively modulating intestinal homeostasis.
32316544	1	17	theme	"	187:187	arg1	sweeteners					117:126	Non-caloric artificial sweeteners	94:126	Non-caloric artificial sweeteners	94:126	Non-caloric artificial sweeteners are frequently discussed as components of the "Western diet", negatively modulating intestinal homeostasis.
32316544	9	18	theme	oral	1387:1390	arg1	intake					1402:1407	oral saccharin intake	1387:1407	oral saccharin intake	1387:1407	Together, this study demonstrates that oral saccharin intake decreases intestinal bacteria count and hence encompasses the capacity to reduce acute and chronic colitis activity in mice.
32316544	1	19	theme	Non-caloric	94:104	arg1	components					156:165	components	156:165	components of the "Western diet"	156:187	Non-caloric artificial sweeteners are frequently discussed as components of the "Western diet", negatively modulating intestinal homeostasis.
32316544	1	19	theme	Non-caloric	94:104	arg1	sweeteners					117:126	Non-caloric artificial sweeteners	94:126	Non-caloric artificial sweeteners	94:126	Non-caloric artificial sweeteners are frequently discussed as components of the "Western diet", negatively modulating intestinal homeostasis.
32316544	0	20	theme	Saccharin	0:8	arg1	Supplementation					10:24	Saccharin Supplementation	0:24	Saccharin Supplementation	0:24	Saccharin Supplementation Inhibits Bacterial Growth and Reduces Experimental Colitis in Mice.
32316544	7	21	theme	intestinal	1168:1177	arg1	barrier					1179:1185	the intestinal barrier	1164:1185	the intestinal barrier	1164:1185	In vivo, oral saccharin intake reduced fecal bacterial load and altered microbiome composition, while the intestinal barrier was not obviously affected.
32316544	7	22	theme	fecal	1101:1105	arg1	load					1117:1120	fecal bacterial load	1101:1120	fecal bacterial load	1101:1120	In vivo, oral saccharin intake reduced fecal bacterial load and altered microbiome composition, while the intestinal barrier was not obviously affected.
32316544	5	23	theme	quantitative	882:893	arg1	analyses					872:879	immunohistochemistry analyses	851:879	immunohistochemistry analyses	851:879	Ex vivo, intestinal inflammation, fecal bacterial load and composition were studied by immunohistochemistry analyses, quantitative PCR, 16 S RNA PCR or next generation sequencing in samples collected from analyzed mice.
32316544	5	23	theme	quantitative	882:893	arg1	PCR					895:897	quantitative PCR	882:897	quantitative PCR	882:897	Ex vivo, intestinal inflammation, fecal bacterial load and composition were studied by immunohistochemistry analyses, quantitative PCR, 16 S RNA PCR or next generation sequencing in samples collected from analyzed mice.
32316544	1	24	theme	artificial	106:115	arg1	components					156:165	components	156:165	components of the "Western diet"	156:187	Non-caloric artificial sweeteners are frequently discussed as components of the "Western diet", negatively modulating intestinal homeostasis.
32316544	1	24	theme	artificial	106:115	arg1	sweeteners					117:126	Non-caloric artificial sweeteners	94:126	Non-caloric artificial sweeteners	94:126	Non-caloric artificial sweeteners are frequently discussed as components of the "Western diet", negatively modulating intestinal homeostasis.
32316544	4	25	theme	sodium	703:708	arg1	DSS					719:721	DSS	719:721	DSS	719:721	Mice were supplemented with saccharin before or after induction of acute or chronic colitis using dextran sodium sulfate (DSS) and the extent of colitis was assessed.
32316544	4	25	theme	sodium	703:708	arg1	sulfate					710:716	dextran sodium sulfate	695:716	dextran sodium sulfate (DSS)	695:722	Mice were supplemented with saccharin before or after induction of acute or chronic colitis using dextran sodium sulfate (DSS) and the extent of colitis was assessed.
32316544	0	26	theme	Bacterial	35:43	arg1	Growth					45:50	Bacterial Growth	35:50	Bacterial Growth	35:50	Saccharin Supplementation Inhibits Bacterial Growth and Reduces Experimental Colitis in Mice.
32316544	7	27	theme	saccharin	1076:1084	arg1	intake					1086:1091	oral saccharin intake	1071:1091	oral saccharin intake	1071:1091	In vivo, oral saccharin intake reduced fecal bacterial load and altered microbiome composition, while the intestinal barrier was not obviously affected.
32316544	3	28	theme	saccharin	586:594	arg1	absence					575:581	absence	575:581	absence	575:581	In vitro, different bacterial strains were grown in the presence or absence of saccharin.
32316544	3	28	theme	saccharin	586:594	arg1	presence					563:570	presence	563:570	presence	563:570	In vitro, different bacterial strains were grown in the presence or absence of saccharin.
32316544	9	29	theme	colitis	1508:1514	arg1	activity					1516:1523	acute and chronic colitis activity	1490:1523	acute and chronic colitis activity in mice	1490:1531	Together, this study demonstrates that oral saccharin intake decreases intestinal bacteria count and hence encompasses the capacity to reduce acute and chronic colitis activity in mice.
32316544	2	30	theme	chronic	473:479	arg1	activity					489:496	acute and chronic colitis activity	463:496	acute and chronic colitis activity in mice	463:504	Since the artificial sweetener saccharin is known to depict bacteriostatic and microbiome-modulating properties, we hypothesized oral saccharin intake to influence intestinal inflammation and aimed at delineating its effect on acute and chronic colitis activity in mice.
32316544	5	31	theme	analyzed	969:976	arg1	mice					978:981	analyzed mice	969:981	analyzed mice	969:981	Ex vivo, intestinal inflammation, fecal bacterial load and composition were studied by immunohistochemistry analyses, quantitative PCR, 16 S RNA PCR or next generation sequencing in samples collected from analyzed mice.
32316544	1	32	theme	intestinal	212:221	arg1	homeostasis					223:233	intestinal homeostasis	212:233	intestinal homeostasis	212:233	Non-caloric artificial sweeteners are frequently discussed as components of the "Western diet", negatively modulating intestinal homeostasis.
32316544	8	33	theme	DSS-induced	1224:1234	arg1	colitis					1236:1242	DSS-induced colitis	1224:1242	DSS-induced colitis activity	1224:1251	Of note, DSS-induced colitis activity was significantly improved in mice after therapeutic or prophylactic treatment with saccharin.
32316544	8	34	theme	prophylactic	1309:1320	arg1	treatment					1322:1330	therapeutic or prophylactic treatment	1294:1330	therapeutic or prophylactic treatment with saccharin	1294:1345	Of note, DSS-induced colitis activity was significantly improved in mice after therapeutic or prophylactic treatment with saccharin.
32316544	2	35	theme	sweetener	257:265	arg1	saccharin					267:275	the artificial sweetener saccharin	242:275	the artificial sweetener saccharin	242:275	Since the artificial sweetener saccharin is known to depict bacteriostatic and microbiome-modulating properties, we hypothesized oral saccharin intake to influence intestinal inflammation and aimed at delineating its effect on acute and chronic colitis activity in mice.
32316544	5	36	theme	16	900:901	arg1	S					903:903	S	903:903	S	903:903	Ex vivo, intestinal inflammation, fecal bacterial load and composition were studied by immunohistochemistry analyses, quantitative PCR, 16 S RNA PCR or next generation sequencing in samples collected from analyzed mice.
32316544	4	37	theme	colitis	681:687	arg1	induction					651:659	induction	651:659	induction of acute or chronic colitis using dextran sodium sulfate (DSS)	651:722	Mice were supplemented with saccharin before or after induction of acute or chronic colitis using dextran sodium sulfate (DSS) and the extent of colitis was assessed.
32316544	4	38	theme	dextran	695:701	arg1	DSS					719:721	DSS	719:721	DSS	719:721	Mice were supplemented with saccharin before or after induction of acute or chronic colitis using dextran sodium sulfate (DSS) and the extent of colitis was assessed.
32316544	4	38	theme	dextran	695:701	arg1	sulfate					710:716	dextran sodium sulfate	695:716	dextran sodium sulfate (DSS)	695:722	Mice were supplemented with saccharin before or after induction of acute or chronic colitis using dextran sodium sulfate (DSS) and the extent of colitis was assessed.
32316544	9	39	theme	saccharin	1392:1400	arg1	intake					1402:1407	oral saccharin intake	1387:1407	oral saccharin intake	1387:1407	Together, this study demonstrates that oral saccharin intake decreases intestinal bacteria count and hence encompasses the capacity to reduce acute and chronic colitis activity in mice.
32316544	5	40	theme	fecal	798:802	arg1	load					814:817	fecal bacterial load	798:817	fecal bacterial load	798:817	Ex vivo, intestinal inflammation, fecal bacterial load and composition were studied by immunohistochemistry analyses, quantitative PCR, 16 S RNA PCR or next generation sequencing in samples collected from analyzed mice.
32316544	8	41	with	treatment	1322:1330	arg1	saccharin					1337:1345	saccharin	1337:1345	saccharin	1337:1345	Of note, DSS-induced colitis activity was significantly improved in mice after therapeutic or prophylactic treatment with saccharin.
32316544	2	42	theme	artificial	246:255	arg1	saccharin					267:275	the artificial sweetener saccharin	242:275	the artificial sweetener saccharin	242:275	Since the artificial sweetener saccharin is known to depict bacteriostatic and microbiome-modulating properties, we hypothesized oral saccharin intake to influence intestinal inflammation and aimed at delineating its effect on acute and chronic colitis activity in mice.
32316544	4	43	theme	chronic	673:679	arg1	colitis					681:687	acute or chronic colitis	664:687	acute or chronic colitis using dextran sodium sulfate (DSS)	664:722	Mice were supplemented with saccharin before or after induction of acute or chronic colitis using dextran sodium sulfate (DSS) and the extent of colitis was assessed.
32316544	0	44	theme	Experimental	64:75	arg1	Colitis					77:83	Experimental Colitis	64:83	Experimental Colitis in Mice	64:91	Saccharin Supplementation Inhibits Bacterial Growth and Reduces Experimental Colitis in Mice.
32316544	0	45	from	Colitis	77:83	arg1	Mice					88:91	Mice	88:91	Mice	88:91	Saccharin Supplementation Inhibits Bacterial Growth and Reduces Experimental Colitis in Mice.
32316544	3	46	theme	bacterial	527:535	arg1	strains					537:543	different bacterial strains	517:543	different bacterial strains	517:543	In vitro, different bacterial strains were grown in the presence or absence of saccharin.
32316544	2	47	theme	colitis	481:487	arg1	activity					489:496	acute and chronic colitis activity	463:496	acute and chronic colitis activity in mice	463:504	Since the artificial sweetener saccharin is known to depict bacteriostatic and microbiome-modulating properties, we hypothesized oral saccharin intake to influence intestinal inflammation and aimed at delineating its effect on acute and chronic colitis activity in mice.
32316544	5	48	theme	generation	921:930	arg1	analyses					872:879	immunohistochemistry analyses	851:879	immunohistochemistry analyses	851:879	Ex vivo, intestinal inflammation, fecal bacterial load and composition were studied by immunohistochemistry analyses, quantitative PCR, 16 S RNA PCR or next generation sequencing in samples collected from analyzed mice.
32316544	5	48	theme	generation	921:930	arg1	sequencing					932:941	next generation sequencing	916:941	next generation sequencing	916:941	Ex vivo, intestinal inflammation, fecal bacterial load and composition were studied by immunohistochemistry analyses, quantitative PCR, 16 S RNA PCR or next generation sequencing in samples collected from analyzed mice.
32316544	9	49	theme	intestinal	1419:1428	arg1	bacteria					1430:1437	intestinal bacteria	1419:1437	intestinal bacteria count	1419:1443	Together, this study demonstrates that oral saccharin intake decreases intestinal bacteria count and hence encompasses the capacity to reduce acute and chronic colitis activity in mice.
32316544	7	50	theme	bacterial	1107:1115	arg1	load					1117:1120	fecal bacterial load	1101:1120	fecal bacterial load	1101:1120	In vivo, oral saccharin intake reduced fecal bacterial load and altered microbiome composition, while the intestinal barrier was not obviously affected.
32316544	2	51	theme	saccharin	370:378	arg1	intake					380:385	oral saccharin intake	365:385	oral saccharin intake	365:385	Since the artificial sweetener saccharin is known to depict bacteriostatic and microbiome-modulating properties, we hypothesized oral saccharin intake to influence intestinal inflammation and aimed at delineating its effect on acute and chronic colitis activity in mice.
32316544	9	52	theme	bacteria	1430:1437	arg1	count					1439:1443	intestinal bacteria count	1419:1443	intestinal bacteria count	1419:1443	Together, this study demonstrates that oral saccharin intake decreases intestinal bacteria count and hence encompasses the capacity to reduce acute and chronic colitis activity in mice.
32316544	6	53	theme	species-dependent	1036:1052	arg1	manner					1054:1059	a species-dependent manner	1034:1059	a species-dependent manner	1034:1059	In vitro, saccharin inhibited bacterial growth in a species-dependent manner.
32316544	9	54	from	activity	1516:1523	arg1	mice					1528:1531	mice	1528:1531	mice	1528:1531	Together, this study demonstrates that oral saccharin intake decreases intestinal bacteria count and hence encompasses the capacity to reduce acute and chronic colitis activity in mice.
32316544	5	55	theme	next	916:919	arg1	analyses					872:879	immunohistochemistry analyses	851:879	immunohistochemistry analyses	851:879	Ex vivo, intestinal inflammation, fecal bacterial load and composition were studied by immunohistochemistry analyses, quantitative PCR, 16 S RNA PCR or next generation sequencing in samples collected from analyzed mice.
32316544	5	55	theme	next	916:919	arg1	sequencing					932:941	next generation sequencing	916:941	next generation sequencing	916:941	Ex vivo, intestinal inflammation, fecal bacterial load and composition were studied by immunohistochemistry analyses, quantitative PCR, 16 S RNA PCR or next generation sequencing in samples collected from analyzed mice.
32316544	2	56	theme	oral	365:368	arg1	intake					380:385	oral saccharin intake	365:385	oral saccharin intake	365:385	Since the artificial sweetener saccharin is known to depict bacteriostatic and microbiome-modulating properties, we hypothesized oral saccharin intake to influence intestinal inflammation and aimed at delineating its effect on acute and chronic colitis activity in mice.
32316544	4	57	theme	colitis	742:748	arg1	extent					732:737	the extent	728:737	the extent of colitis	728:748	Mice were supplemented with saccharin before or after induction of acute or chronic colitis using dextran sodium sulfate (DSS) and the extent of colitis was assessed.
32316544	8	58	theme	therapeutic	1294:1304	arg1	treatment					1322:1330	therapeutic or prophylactic treatment	1294:1330	therapeutic or prophylactic treatment with saccharin	1294:1345	Of note, DSS-induced colitis activity was significantly improved in mice after therapeutic or prophylactic treatment with saccharin.
32316544	7	59	theme	oral	1071:1074	arg1	intake					1086:1091	oral saccharin intake	1071:1091	oral saccharin intake	1071:1091	In vivo, oral saccharin intake reduced fecal bacterial load and altered microbiome composition, while the intestinal barrier was not obviously affected.
32316544	2	60	from	effect	453:458	arg1	activity					489:496	acute and chronic colitis activity	463:496	acute and chronic colitis activity in mice	463:504	Since the artificial sweetener saccharin is known to depict bacteriostatic and microbiome-modulating properties, we hypothesized oral saccharin intake to influence intestinal inflammation and aimed at delineating its effect on acute and chronic colitis activity in mice.
32316544	5	61	theme	S	903:903	arg1	PCR					909:911	16 S RNA PCR	900:911	16 S RNA PCR	900:911	Ex vivo, intestinal inflammation, fecal bacterial load and composition were studied by immunohistochemistry analyses, quantitative PCR, 16 S RNA PCR or next generation sequencing in samples collected from analyzed mice.
32316544	5	61	theme	S	903:903	arg1	analyses					872:879	immunohistochemistry analyses	851:879	immunohistochemistry analyses	851:879	Ex vivo, intestinal inflammation, fecal bacterial load and composition were studied by immunohistochemistry analyses, quantitative PCR, 16 S RNA PCR or next generation sequencing in samples collected from analyzed mice.
32316544	9	62	theme	acute	1490:1494	arg1	activity					1516:1523	acute and chronic colitis activity	1490:1523	acute and chronic colitis activity in mice	1490:1531	Together, this study demonstrates that oral saccharin intake decreases intestinal bacteria count and hence encompasses the capacity to reduce acute and chronic colitis activity in mice.
32316544	5	63	theme	bacterial	804:812	arg1	load					814:817	fecal bacterial load	798:817	fecal bacterial load	798:817	Ex vivo, intestinal inflammation, fecal bacterial load and composition were studied by immunohistochemistry analyses, quantitative PCR, 16 S RNA PCR or next generation sequencing in samples collected from analyzed mice.
32316544	5	64	theme	RNA	905:907	arg1	PCR					909:911	16 S RNA PCR	900:911	16 S RNA PCR	900:911	Ex vivo, intestinal inflammation, fecal bacterial load and composition were studied by immunohistochemistry analyses, quantitative PCR, 16 S RNA PCR or next generation sequencing in samples collected from analyzed mice.
32316544	5	64	theme	RNA	905:907	arg1	analyses					872:879	immunohistochemistry analyses	851:879	immunohistochemistry analyses	851:879	Ex vivo, intestinal inflammation, fecal bacterial load and composition were studied by immunohistochemistry analyses, quantitative PCR, 16 S RNA PCR or next generation sequencing in samples collected from analyzed mice.
33800788	6	0	dep	efficiency	988:997	arg1	%					972:972	%	972:972	%	972:972	The optimized formulation resulted in 90 ± 3.6% encapsulation efficiency, 167.9 ± 3.5 nm average hydrodynamic diameter, 0.129 ± 0.002 PDI, and -33.9 ± 4.5 zeta potential.
33800788	7	1	theme	Coated	1097:1102	arg1	liposomes					1104:1112	Coated liposomes	1097:1112	Coated liposomes	1097:1112	Coated liposomes showed significantly improved properties in 24 h in an in vitro release test (>60%), in vitro permeability measurement (420 μg/cm2) within 60 min, and also in accelerated stability studies compared to uncoated liposomes.
33800788	5	2	theme	optimized	893:901	arg1	formulation					913:923	the optimized liposomal formulation	889:923	the optimized liposomal formulation	889:923	Physiochemical characterization, in vitro release, and permeability tests, as well as accelerated stability studies, were performed with the optimized liposomal formulation.
33800788	8	3	theme	PG-containing	1401:1413	arg1	liposomes					1415:1423	PG-containing liposomes	1401:1423	PG-containing liposomes	1401:1423	A hydrogen-peroxide-scavenging assay showed improved stability of PG-containing liposomes.
33800788	5	4	theme	in	785:786	arg1	release					794:800	in vitro release	785:800	in vitro release	785:800	Physiochemical characterization, in vitro release, and permeability tests, as well as accelerated stability studies, were performed with the optimized liposomal formulation.
33800788	3	5	theme	PG-loaded	511:519	arg1	liposomes					521:529	PG-loaded liposomes	511:529	PG-loaded liposomes coated with HA	511:544	The QbD method was used for the determination of critical quality attributes in the formulation of PG-loaded liposomes coated with HA.
33800788	6	6	theme	167.9	1000:1004	arg1	±					1006:1006	±	1006:1006	±	1006:1006	The optimized formulation resulted in 90 ± 3.6% encapsulation efficiency, 167.9 ± 3.5 nm average hydrodynamic diameter, 0.129 ± 0.002 PDI, and -33.9 ± 4.5 zeta potential.
33800788	6	7	theme	±	1075:1075	arg1	potential					1086:1094	-33.9 ± 4.5 zeta potential	1069:1094	-33.9 ± 4.5 zeta potential	1069:1094	The optimized formulation resulted in 90 ± 3.6% encapsulation efficiency, 167.9 ± 3.5 nm average hydrodynamic diameter, 0.129 ± 0.002 PDI, and -33.9 ± 4.5 zeta potential.
33800788	7	8	from	properties	1144:1153	arg1	h					1161:1161	24 h	1158:1161	24 h in an in vitro release test (>60%), in vitro permeability measurement (420 μg/cm2)	1158:1244	Coated liposomes showed significantly improved properties in 24 h in an in vitro release test (>60%), in vitro permeability measurement (420 μg/cm2) within 60 min, and also in accelerated stability studies compared to uncoated liposomes.
33800788	3	9	used	used	431:434	arg2	method					420:425	The QbD method	412:425	The QbD method	412:425	The QbD method was used for the determination of critical quality attributes in the formulation of PG-loaded liposomes coated with HA.
33800788	4	10	theme	Box-Behnken	604:614	arg1	design					616:621	the Box-Behnken design	600:621	the Box-Behnken design	600:621	The optimized formulation was determined by applying the Box-Behnken design to investigate the effect of composition and process variables on particle size, polydispersity index (PDI), and zeta potential.
33800788	7	11	theme	release	1178:1184	arg1	measurement					1221:1231	in vitro permeability measurement	1199:1231	in vitro permeability measurement (420 μg/cm2)	1199:1244	Coated liposomes showed significantly improved properties in 24 h in an in vitro release test (>60%), in vitro permeability measurement (420 μg/cm2) within 60 min, and also in accelerated stability studies compared to uncoated liposomes.
33800788	7	11	theme	release	1178:1184	arg1	test					1186:1189	an in vitro release test	1166:1189	an in vitro release test (>60%)	1166:1196	Coated liposomes showed significantly improved properties in 24 h in an in vitro release test (>60%), in vitro permeability measurement (420 μg/cm2) within 60 min, and also in accelerated stability studies compared to uncoated liposomes.
33800788	7	11	theme	release	1178:1184	arg1	%					1195:1195	>60%	1192:1195	>60%	1192:1195	Coated liposomes showed significantly improved properties in 24 h in an in vitro release test (>60%), in vitro permeability measurement (420 μg/cm2) within 60 min, and also in accelerated stability studies compared to uncoated liposomes.
33800788	6	12	dep	nm	1012:1013	arg1	±					1006:1006	±	1006:1006	±	1006:1006	The optimized formulation resulted in 90 ± 3.6% encapsulation efficiency, 167.9 ± 3.5 nm average hydrodynamic diameter, 0.129 ± 0.002 PDI, and -33.9 ± 4.5 zeta potential.
33800788	6	13	dep	diameter	1036:1043	arg1	nm					1012:1013	nm	1012:1013	nm	1012:1013	The optimized formulation resulted in 90 ± 3.6% encapsulation efficiency, 167.9 ± 3.5 nm average hydrodynamic diameter, 0.129 ± 0.002 PDI, and -33.9 ± 4.5 zeta potential.
33800788	7	14	theme	improved	1135:1142	arg1	properties					1144:1153	significantly improved properties	1121:1153	significantly improved properties in 24 h in an in vitro release test (>60%), in vitro permeability measurement (420 μg/cm2)	1121:1244	Coated liposomes showed significantly improved properties in 24 h in an in vitro release test (>60%), in vitro permeability measurement (420 μg/cm2) within 60 min, and also in accelerated stability studies compared to uncoated liposomes.
33800788	2	15	theme	coat	310:313	arg1	liposomes					315:323	coat liposomes	310:323	coat liposomes with hyaluronic acid (HA)	310:349	A further aim was to coat liposomes with hyaluronic acid (HA) to improve the stability of the formulation in nasal mucosa.
33800788	2	15	theme	coat	310:313	arg1	aim					299:301	A further aim	289:301	A further aim	289:301	A further aim was to coat liposomes with hyaluronic acid (HA) to improve the stability of the formulation in nasal mucosa.
33800788	4	16	theme	particle	689:696	arg1	size					698:701	particle size	689:701	particle size	689:701	The optimized formulation was determined by applying the Box-Behnken design to investigate the effect of composition and process variables on particle size, polydispersity index (PDI), and zeta potential.
33800788	4	17	theme	variables	676:684	arg1	effect					642:647	the effect	638:647	the effect of composition and process variables on particle size, polydispersity index (PDI), and zeta potential	638:749	The optimized formulation was determined by applying the Box-Behnken design to investigate the effect of composition and process variables on particle size, polydispersity index (PDI), and zeta potential.
33800788	7	18	theme	in	1169:1170	arg1	measurement					1221:1231	in vitro permeability measurement	1199:1231	in vitro permeability measurement (420 μg/cm2)	1199:1244	Coated liposomes showed significantly improved properties in 24 h in an in vitro release test (>60%), in vitro permeability measurement (420 μg/cm2) within 60 min, and also in accelerated stability studies compared to uncoated liposomes.
33800788	7	18	theme	in	1169:1170	arg1	test					1186:1189	an in vitro release test	1166:1189	an in vitro release test (>60%)	1166:1196	Coated liposomes showed significantly improved properties in 24 h in an in vitro release test (>60%), in vitro permeability measurement (420 μg/cm2) within 60 min, and also in accelerated stability studies compared to uncoated liposomes.
33800788	7	18	theme	in	1169:1170	arg1	%					1195:1195	>60%	1192:1195	>60%	1192:1195	Coated liposomes showed significantly improved properties in 24 h in an in vitro release test (>60%), in vitro permeability measurement (420 μg/cm2) within 60 min, and also in accelerated stability studies compared to uncoated liposomes.
33800788	5	19	theme	liposomal	903:911	arg1	formulation					913:923	the optimized liposomal formulation	889:923	the optimized liposomal formulation	889:923	Physiochemical characterization, in vitro release, and permeability tests, as well as accelerated stability studies, were performed with the optimized liposomal formulation.
33800788	0	20	theme	Liposomes	86:94	arg1	Development					24:34	Quality-by-Design-Based Development	0:34	Quality-by-Design-Based Development of n-Propyl-Gallate-Loaded Hyaluronic-Acid-Coated Liposomes for Intranasal Administration.	0:125	Quality-by-Design-Based Development of n-Propyl-Gallate-Loaded Hyaluronic-Acid-Coated Liposomes for Intranasal Administration.
33800788	2	21	from	stability	366:374	arg1	mucosa					404:409	nasal mucosa	398:409	nasal mucosa	398:409	A further aim was to coat liposomes with hyaluronic acid (HA) to improve the stability of the formulation in nasal mucosa.
33800788	1	22	theme	present	131:137	arg1	study					139:143	The present study	127:143	The present study	127:143	The present study aimed to develop n-propyl gallate (PG)-encapsulated liposomes through a novel direct pouring method using the quality-by-design (QbD) approach.
33800788	2	23	theme	nasal	398:402	arg1	mucosa					404:409	nasal mucosa	398:409	nasal mucosa	398:409	A further aim was to coat liposomes with hyaluronic acid (HA) to improve the stability of the formulation in nasal mucosa.
33800788	5	24	theme	permeability	807:818	arg1	tests					820:824	permeability tests	807:824	permeability tests	807:824	Physiochemical characterization, in vitro release, and permeability tests, as well as accelerated stability studies, were performed with the optimized liposomal formulation.
33800788	5	25	theme	stability	850:858	arg1	studies					860:866	accelerated stability studies	838:866	accelerated stability studies	838:866	Physiochemical characterization, in vitro release, and permeability tests, as well as accelerated stability studies, were performed with the optimized liposomal formulation.
33800788	4	26	theme	process	668:674	arg1	variables					676:684	process variables	668:684	process variables	668:684	The optimized formulation was determined by applying the Box-Behnken design to investigate the effect of composition and process variables on particle size, polydispersity index (PDI), and zeta potential.
33800788	4	27	theme	zeta	736:739	arg1	potential					741:749	zeta potential	736:749	zeta potential	736:749	The optimized formulation was determined by applying the Box-Behnken design to investigate the effect of composition and process variables on particle size, polydispersity index (PDI), and zeta potential.
33800788	7	28	dep	in	1169:1170	arg1	vitro					1172:1176	vitro	1172:1176	vitro	1172:1176	Coated liposomes showed significantly improved properties in 24 h in an in vitro release test (>60%), in vitro permeability measurement (420 μg/cm2) within 60 min, and also in accelerated stability studies compared to uncoated liposomes.
33800788	1	29	theme	direct	223:228	arg1	method					238:243	a novel direct pouring method	215:243	a novel direct pouring method using the quality-by-design (QbD) approach	215:286	The present study aimed to develop n-propyl gallate (PG)-encapsulated liposomes through a novel direct pouring method using the quality-by-design (QbD) approach.
33800788	0	30	theme	Quality-by-Design-Based	0:22	arg1	Development					24:34	Quality-by-Design-Based Development	0:34	Quality-by-Design-Based Development of n-Propyl-Gallate-Loaded Hyaluronic-Acid-Coated Liposomes for Intranasal Administration.	0:125	Quality-by-Design-Based Development of n-Propyl-Gallate-Loaded Hyaluronic-Acid-Coated Liposomes for Intranasal Administration.
33800788	6	31	theme	encapsulation	974:986	arg1	efficiency					988:997	90 ± 3.6% encapsulation efficiency	964:997	90 ± 3.6% encapsulation efficiency	964:997	The optimized formulation resulted in 90 ± 3.6% encapsulation efficiency, 167.9 ± 3.5 nm average hydrodynamic diameter, 0.129 ± 0.002 PDI, and -33.9 ± 4.5 zeta potential.
33800788	6	32	dep	%	972:972	arg1	±					967:967	±	967:967	±	967:967	The optimized formulation resulted in 90 ± 3.6% encapsulation efficiency, 167.9 ± 3.5 nm average hydrodynamic diameter, 0.129 ± 0.002 PDI, and -33.9 ± 4.5 zeta potential.
33800788	9	33	theme	liposomes	1487:1495	arg1	optimization					1455:1466	the optimization	1451:1466	the optimization of PG-encapsulated liposomes coated with HA	1451:1510	It can be concluded that the optimization of PG-encapsulated liposomes coated with HA has great potential for targeting several brain diseases.
33800788	2	34	theme	formulation	383:393	arg1	stability					366:374	the stability	362:374	the stability of the formulation in nasal mucosa	362:409	A further aim was to coat liposomes with hyaluronic acid (HA) to improve the stability of the formulation in nasal mucosa.
33800788	0	35	theme	n-Propyl-Gallate-Loaded	39:61	arg1	Liposomes					86:94	n-Propyl-Gallate-Loaded Hyaluronic-Acid-Coated Liposomes	39:94	n-Propyl-Gallate-Loaded Hyaluronic-Acid-Coated Liposomes	39:94	Quality-by-Design-Based Development of n-Propyl-Gallate-Loaded Hyaluronic-Acid-Coated Liposomes for Intranasal Administration.
33800788	4	36	theme	optimized	551:559	arg1	formulation					561:571	The optimized formulation	547:571	The optimized formulation	547:571	The optimized formulation was determined by applying the Box-Behnken design to investigate the effect of composition and process variables on particle size, polydispersity index (PDI), and zeta potential.
33800788	2	37	theme	hyaluronic	330:339	arg1	HA					347:348	HA	347:348	HA	347:348	A further aim was to coat liposomes with hyaluronic acid (HA) to improve the stability of the formulation in nasal mucosa.
33800788	2	37	theme	hyaluronic	330:339	arg1	acid					341:344	hyaluronic acid	330:344	hyaluronic acid (HA)	330:349	A further aim was to coat liposomes with hyaluronic acid (HA) to improve the stability of the formulation in nasal mucosa.
33800788	3	38	theme	critical	461:468	arg1	attributes					478:487	critical quality attributes	461:487	critical quality attributes	461:487	The QbD method was used for the determination of critical quality attributes in the formulation of PG-loaded liposomes coated with HA.
33800788	9	39	theme	several	1546:1552	arg1	diseases					1560:1567	several brain diseases	1546:1567	several brain diseases	1546:1567	It can be concluded that the optimization of PG-encapsulated liposomes coated with HA has great potential for targeting several brain diseases.
33800788	6	40	theme	3.6	969:971	arg1	±					967:967	±	967:967	±	967:967	The optimized formulation resulted in 90 ± 3.6% encapsulation efficiency, 167.9 ± 3.5 nm average hydrodynamic diameter, 0.129 ± 0.002 PDI, and -33.9 ± 4.5 zeta potential.
33800788	5	41	dep	in	785:786	arg1	vitro					788:792	vitro	788:792	vitro	788:792	Physiochemical characterization, in vitro release, and permeability tests, as well as accelerated stability studies, were performed with the optimized liposomal formulation.
33800788	8	42	theme	liposomes	1415:1423	arg1	stability					1388:1396	improved stability	1379:1396	improved stability of PG-containing liposomes	1379:1423	A hydrogen-peroxide-scavenging assay showed improved stability of PG-containing liposomes.
33800788	4	43	theme	polydispersity	704:717	arg1	PDI					726:728	PDI	726:728	PDI	726:728	The optimized formulation was determined by applying the Box-Behnken design to investigate the effect of composition and process variables on particle size, polydispersity index (PDI), and zeta potential.
33800788	4	43	theme	polydispersity	704:717	arg1	index					719:723	polydispersity index	704:723	polydispersity index (PDI)	704:729	The optimized formulation was determined by applying the Box-Behnken design to investigate the effect of composition and process variables on particle size, polydispersity index (PDI), and zeta potential.
33800788	3	44	theme	quality	470:476	arg1	attributes					478:487	critical quality attributes	461:487	critical quality attributes	461:487	The QbD method was used for the determination of critical quality attributes in the formulation of PG-loaded liposomes coated with HA.
33800788	3	45	theme	QbD	416:418	arg1	method					420:425	The QbD method	412:425	The QbD method	412:425	The QbD method was used for the determination of critical quality attributes in the formulation of PG-loaded liposomes coated with HA.
33800788	9	46	contain	has	1512:1514	arg1	optimization					1455:1466	the optimization	1451:1466	the optimization of PG-encapsulated liposomes coated with HA	1451:1510	It can be concluded that the optimization of PG-encapsulated liposomes coated with HA has great potential for targeting several brain diseases.
33800788	9	46	contain	has	1512:1514	arg2	potential					1522:1530	great potential	1516:1530	great potential for targeting several brain diseases	1516:1567	It can be concluded that the optimization of PG-encapsulated liposomes coated with HA has great potential for targeting several brain diseases.
33800788	7	47	theme	in	1199:1200	arg1	measurement					1221:1231	in vitro permeability measurement	1199:1231	in vitro permeability measurement (420 μg/cm2)	1199:1244	Coated liposomes showed significantly improved properties in 24 h in an in vitro release test (>60%), in vitro permeability measurement (420 μg/cm2) within 60 min, and also in accelerated stability studies compared to uncoated liposomes.
33800788	7	47	theme	in	1199:1200	arg1	μg/cm2					1238:1243	420 μg/cm2	1234:1243	420 μg/cm2	1234:1243	Coated liposomes showed significantly improved properties in 24 h in an in vitro release test (>60%), in vitro permeability measurement (420 μg/cm2) within 60 min, and also in accelerated stability studies compared to uncoated liposomes.
33800788	7	47	theme	in	1199:1200	arg1	test					1186:1189	an in vitro release test	1166:1189	an in vitro release test (>60%)	1166:1196	Coated liposomes showed significantly improved properties in 24 h in an in vitro release test (>60%), in vitro permeability measurement (420 μg/cm2) within 60 min, and also in accelerated stability studies compared to uncoated liposomes.
33800788	7	48	theme	uncoated	1315:1322	arg1	liposomes					1324:1332	uncoated liposomes	1315:1332	uncoated liposomes	1315:1332	Coated liposomes showed significantly improved properties in 24 h in an in vitro release test (>60%), in vitro permeability measurement (420 μg/cm2) within 60 min, and also in accelerated stability studies compared to uncoated liposomes.
33800788	1	49	theme	pouring	230:236	arg1	method					238:243	a novel direct pouring method	215:243	a novel direct pouring method using the quality-by-design (QbD) approach	215:286	The present study aimed to develop n-propyl gallate (PG)-encapsulated liposomes through a novel direct pouring method using the quality-by-design (QbD) approach.
33800788	5	50	theme	accelerated	838:848	arg1	studies					860:866	accelerated stability studies	838:866	accelerated stability studies	838:866	Physiochemical characterization, in vitro release, and permeability tests, as well as accelerated stability studies, were performed with the optimized liposomal formulation.
33800788	0	51	theme	Hyaluronic-Acid-Coated	63:84	arg1	Liposomes					86:94	n-Propyl-Gallate-Loaded Hyaluronic-Acid-Coated Liposomes	39:94	n-Propyl-Gallate-Loaded Hyaluronic-Acid-Coated Liposomes	39:94	Quality-by-Design-Based Development of n-Propyl-Gallate-Loaded Hyaluronic-Acid-Coated Liposomes for Intranasal Administration.
33800788	6	52	theme	90	964:965	arg1	±					967:967	±	967:967	±	967:967	The optimized formulation resulted in 90 ± 3.6% encapsulation efficiency, 167.9 ± 3.5 nm average hydrodynamic diameter, 0.129 ± 0.002 PDI, and -33.9 ± 4.5 zeta potential.
33800788	3	53	from	determination	444:456	arg1	formulation					496:506	the formulation	492:506	the formulation of PG-loaded liposomes coated with HA	492:544	The QbD method was used for the determination of critical quality attributes in the formulation of PG-loaded liposomes coated with HA.
33800788	1	54	theme	quality-by-design	255:271	arg1	approach					279:286	the quality-by-design (QbD) approach	251:286	the quality-by-design (QbD) approach	251:286	The present study aimed to develop n-propyl gallate (PG)-encapsulated liposomes through a novel direct pouring method using the quality-by-design (QbD) approach.
33800788	0	55	theme	Intranasal	100:109	arg1	Administration					111:124	Intranasal Administration	100:124	Intranasal Administration	100:124	Quality-by-Design-Based Development of n-Propyl-Gallate-Loaded Hyaluronic-Acid-Coated Liposomes for Intranasal Administration.
33800788	9	56	theme	brain	1554:1558	arg1	diseases					1560:1567	several brain diseases	1546:1567	several brain diseases	1546:1567	It can be concluded that the optimization of PG-encapsulated liposomes coated with HA has great potential for targeting several brain diseases.
33800788	6	57	theme	zeta	1081:1084	arg1	potential					1086:1094	-33.9 ± 4.5 zeta potential	1069:1094	-33.9 ± 4.5 zeta potential	1069:1094	The optimized formulation resulted in 90 ± 3.6% encapsulation efficiency, 167.9 ± 3.5 nm average hydrodynamic diameter, 0.129 ± 0.002 PDI, and -33.9 ± 4.5 zeta potential.
33800788	6	58	theme	optimized	930:938	arg1	formulation					940:950	The optimized formulation	926:950	The optimized formulation	926:950	The optimized formulation resulted in 90 ± 3.6% encapsulation efficiency, 167.9 ± 3.5 nm average hydrodynamic diameter, 0.129 ± 0.002 PDI, and -33.9 ± 4.5 zeta potential.
33800788	8	59	theme	hydrogen-peroxide-scavenging	1337:1364	arg1	assay					1366:1370	A hydrogen-peroxide-scavenging assay	1335:1370	A hydrogen-peroxide-scavenging assay	1335:1370	A hydrogen-peroxide-scavenging assay showed improved stability of PG-containing liposomes.
33800788	9	60	theme	great	1516:1520	arg1	potential					1522:1530	great potential	1516:1530	great potential for targeting several brain diseases	1516:1567	It can be concluded that the optimization of PG-encapsulated liposomes coated with HA has great potential for targeting several brain diseases.
33800788	8	61	theme	improved	1379:1386	arg1	stability					1388:1396	improved stability	1379:1396	improved stability of PG-containing liposomes	1379:1423	A hydrogen-peroxide-scavenging assay showed improved stability of PG-containing liposomes.
33800788	7	62	theme	stability	1285:1293	arg1	studies					1295:1301	accelerated stability studies	1273:1301	accelerated stability studies	1273:1301	Coated liposomes showed significantly improved properties in 24 h in an in vitro release test (>60%), in vitro permeability measurement (420 μg/cm2) within 60 min, and also in accelerated stability studies compared to uncoated liposomes.
33800788	6	63	theme	average	1015:1021	arg1	diameter					1036:1043	167.9 ± 3.5 nm average hydrodynamic diameter	1000:1043	167.9 ± 3.5 nm average hydrodynamic diameter	1000:1043	The optimized formulation resulted in 90 ± 3.6% encapsulation efficiency, 167.9 ± 3.5 nm average hydrodynamic diameter, 0.129 ± 0.002 PDI, and -33.9 ± 4.5 zeta potential.
33800788	3	64	theme	attributes	478:487	arg1	determination					444:456	the determination	440:456	the determination of critical quality attributes in the formulation of PG-loaded liposomes coated with HA	440:544	The QbD method was used for the determination of critical quality attributes in the formulation of PG-loaded liposomes coated with HA.
33800788	7	65	dep	in	1199:1200	arg1	vitro					1202:1206	vitro	1202:1206	vitro	1202:1206	Coated liposomes showed significantly improved properties in 24 h in an in vitro release test (>60%), in vitro permeability measurement (420 μg/cm2) within 60 min, and also in accelerated stability studies compared to uncoated liposomes.
33800788	6	66	theme	hydrodynamic	1023:1034	arg1	diameter					1036:1043	167.9 ± 3.5 nm average hydrodynamic diameter	1000:1043	167.9 ± 3.5 nm average hydrodynamic diameter	1000:1043	The optimized formulation resulted in 90 ± 3.6% encapsulation efficiency, 167.9 ± 3.5 nm average hydrodynamic diameter, 0.129 ± 0.002 PDI, and -33.9 ± 4.5 zeta potential.
33800788	7	67	from	h	1161:1161	arg1	measurement					1221:1231	in vitro permeability measurement	1199:1231	in vitro permeability measurement (420 μg/cm2)	1199:1244	Coated liposomes showed significantly improved properties in 24 h in an in vitro release test (>60%), in vitro permeability measurement (420 μg/cm2) within 60 min, and also in accelerated stability studies compared to uncoated liposomes.
33800788	7	67	from	h	1161:1161	arg1	test					1186:1189	an in vitro release test	1166:1189	an in vitro release test (>60%)	1166:1196	Coated liposomes showed significantly improved properties in 24 h in an in vitro release test (>60%), in vitro permeability measurement (420 μg/cm2) within 60 min, and also in accelerated stability studies compared to uncoated liposomes.
33800788	7	67	from	h	1161:1161	arg1	%					1195:1195	>60%	1192:1195	>60%	1192:1195	Coated liposomes showed significantly improved properties in 24 h in an in vitro release test (>60%), in vitro permeability measurement (420 μg/cm2) within 60 min, and also in accelerated stability studies compared to uncoated liposomes.
33800788	2	68	theme	further	291:297	arg1	liposomes					315:323	coat liposomes	310:323	coat liposomes with hyaluronic acid (HA)	310:349	A further aim was to coat liposomes with hyaluronic acid (HA) to improve the stability of the formulation in nasal mucosa.
33800788	2	68	theme	further	291:297	arg1	aim					299:301	A further aim	289:301	A further aim	289:301	A further aim was to coat liposomes with hyaluronic acid (HA) to improve the stability of the formulation in nasal mucosa.
33800788	9	69	theme	PG-encapsulated	1471:1485	arg1	liposomes					1487:1495	PG-encapsulated liposomes	1471:1495	PG-encapsulated liposomes coated with HA	1471:1510	It can be concluded that the optimization of PG-encapsulated liposomes coated with HA has great potential for targeting several brain diseases.
33800788	1	70	theme	QbD	274:276	arg1	approach					279:286	the quality-by-design (QbD) approach	251:286	the quality-by-design (QbD) approach	251:286	The present study aimed to develop n-propyl gallate (PG)-encapsulated liposomes through a novel direct pouring method using the quality-by-design (QbD) approach.
33800788	5	71	theme	Physiochemical	752:765	arg1	characterization					767:782	Physiochemical characterization	752:782	Physiochemical characterization	752:782	Physiochemical characterization, in vitro release, and permeability tests, as well as accelerated stability studies, were performed with the optimized liposomal formulation.
33800788	4	72	theme	composition	652:662	arg1	effect					642:647	the effect	638:647	the effect of composition and process variables on particle size, polydispersity index (PDI), and zeta potential	638:749	The optimized formulation was determined by applying the Box-Behnken design to investigate the effect of composition and process variables on particle size, polydispersity index (PDI), and zeta potential.
33800788	6	73	theme	±	1052:1052	arg1	PDI					1060:1062	0.129 ± 0.002 PDI	1046:1062	0.129 ± 0.002 PDI	1046:1062	The optimized formulation resulted in 90 ± 3.6% encapsulation efficiency, 167.9 ± 3.5 nm average hydrodynamic diameter, 0.129 ± 0.002 PDI, and -33.9 ± 4.5 zeta potential.
33800788	3	74	theme	liposomes	521:529	arg1	formulation					496:506	the formulation	492:506	the formulation of PG-loaded liposomes coated with HA	492:544	The QbD method was used for the determination of critical quality attributes in the formulation of PG-loaded liposomes coated with HA.
33800788	1	75	theme	novel	217:221	arg1	method					238:243	a novel direct pouring method	215:243	a novel direct pouring method using the quality-by-design (QbD) approach	215:286	The present study aimed to develop n-propyl gallate (PG)-encapsulated liposomes through a novel direct pouring method using the quality-by-design (QbD) approach.
33800788	2	76	with	liposomes	315:323	arg1	HA					347:348	HA	347:348	HA	347:348	A further aim was to coat liposomes with hyaluronic acid (HA) to improve the stability of the formulation in nasal mucosa.
33800788	2	76	with	liposomes	315:323	arg1	acid					341:344	hyaluronic acid	330:344	hyaluronic acid (HA)	330:349	A further aim was to coat liposomes with hyaluronic acid (HA) to improve the stability of the formulation in nasal mucosa.
33800788	4	77	from	effect	642:647	arg1	potential					741:749	zeta potential	736:749	zeta potential	736:749	The optimized formulation was determined by applying the Box-Behnken design to investigate the effect of composition and process variables on particle size, polydispersity index (PDI), and zeta potential.
33800788	4	77	from	effect	642:647	arg1	PDI					726:728	PDI	726:728	PDI	726:728	The optimized formulation was determined by applying the Box-Behnken design to investigate the effect of composition and process variables on particle size, polydispersity index (PDI), and zeta potential.
33800788	4	77	from	effect	642:647	arg1	size					698:701	particle size	689:701	particle size	689:701	The optimized formulation was determined by applying the Box-Behnken design to investigate the effect of composition and process variables on particle size, polydispersity index (PDI), and zeta potential.
33800788	4	77	from	effect	642:647	arg1	index					719:723	polydispersity index	704:723	polydispersity index (PDI)	704:729	The optimized formulation was determined by applying the Box-Behnken design to investigate the effect of composition and process variables on particle size, polydispersity index (PDI), and zeta potential.
33800788	7	78	theme	accelerated	1273:1283	arg1	studies					1295:1301	accelerated stability studies	1273:1301	accelerated stability studies	1273:1301	Coated liposomes showed significantly improved properties in 24 h in an in vitro release test (>60%), in vitro permeability measurement (420 μg/cm2) within 60 min, and also in accelerated stability studies compared to uncoated liposomes.
33800788	7	79	theme	permeability	1208:1219	arg1	measurement					1221:1231	in vitro permeability measurement	1199:1231	in vitro permeability measurement (420 μg/cm2)	1199:1244	Coated liposomes showed significantly improved properties in 24 h in an in vitro release test (>60%), in vitro permeability measurement (420 μg/cm2) within 60 min, and also in accelerated stability studies compared to uncoated liposomes.
33800788	7	79	theme	permeability	1208:1219	arg1	μg/cm2					1238:1243	420 μg/cm2	1234:1243	420 μg/cm2	1234:1243	Coated liposomes showed significantly improved properties in 24 h in an in vitro release test (>60%), in vitro permeability measurement (420 μg/cm2) within 60 min, and also in accelerated stability studies compared to uncoated liposomes.
33800788	7	79	theme	permeability	1208:1219	arg1	test					1186:1189	an in vitro release test	1166:1189	an in vitro release test (>60%)	1166:1196	Coated liposomes showed significantly improved properties in 24 h in an in vitro release test (>60%), in vitro permeability measurement (420 μg/cm2) within 60 min, and also in accelerated stability studies compared to uncoated liposomes.
33800788	1	80	theme	-encapsulated	183:195	arg1	liposomes					197:205	n-propyl gallate (PG)-encapsulated liposomes	162:205	n-propyl gallate (PG)-encapsulated liposomes	162:205	The present study aimed to develop n-propyl gallate (PG)-encapsulated liposomes through a novel direct pouring method using the quality-by-design (QbD) approach.
33800788	6	81	theme	3.5	1008:1010	arg1	±					1006:1006	±	1006:1006	±	1006:1006	The optimized formulation resulted in 90 ± 3.6% encapsulation efficiency, 167.9 ± 3.5 nm average hydrodynamic diameter, 0.129 ± 0.002 PDI, and -33.9 ± 4.5 zeta potential.
31616940	4	0	dep	°C	696:697	arg1	to					699:700	to	699:700	to	699:700	Exposure to low temperatures induces the expression of galactinol synthase (AnGolS1) in Ammopiptanthus nanus, a desert plant that survives temperatures between -30 °C to 47 °C. AnGolS1 has a greater catalytic activity than tomato galactinol synthase (SlGolS2).
31616940	7	1	theme	transgenic	980:989	arg1	lines					998:1002	AnGolS1 transgenic tomato lines	972:1002	AnGolS1 transgenic tomato lines	972:1002	AnGolS1 transgenic tomato lines exhibited an enhanced capacity for ethylene (ET) signaling.
31616940	8	2	theme	galactinol	1083:1092	arg1	application					1068:1078	The application	1064:1078	The application of galactinol	1064:1092	The application of galactinol abolished the repression of the ET signaling pathway by 1-methylcyclopropene during seed germination.
31616940	8	3	theme	seed	1178:1181	arg1	germination					1183:1193	seed germination	1178:1193	seed germination	1178:1193	The application of galactinol abolished the repression of the ET signaling pathway by 1-methylcyclopropene during seed germination.
31616940	2	4	theme	important	334:342	arg1	role					344:347	an important role	331:347	an important role	331:347	Raffinose family oligosaccharides (RFOs) are an example and play an important role in abiotic stress tolerance.
31616940	1	5	theme	cold	253:256	arg1	stress					258:263	cold stress	253:263	cold stress	253:263	Soluble carbohydrates not only directly affect plant growth and development but also act as signal molecules in processes that enhance tolerance to cold stress.
31616940	4	6	from	expression	573:582	arg1	nanus					635:639	Ammopiptanthus nanus	620:639	Ammopiptanthus nanus	620:639	Exposure to low temperatures induces the expression of galactinol synthase (AnGolS1) in Ammopiptanthus nanus, a desert plant that survives temperatures between -30 °C to 47 °C. AnGolS1 has a greater catalytic activity than tomato galactinol synthase (SlGolS2).
31616940	0	7	from	Expression	0:9	arg1	tomato					63:68	tomato	63:68	tomato	63:68	Expression of galactinol synthase from Ammopiptanthus nanus in tomato improves tolerance to cold stress.
31616940	0	7	from	Expression	0:9	arg1	nanus					54:58	Ammopiptanthus nanus	39:58	Ammopiptanthus nanus	39:58	Expression of galactinol synthase from Ammopiptanthus nanus in tomato improves tolerance to cold stress.
31616940	3	8	theme	cold	516:519	arg1	tolerance					521:529	cold tolerance	516:529	cold tolerance	516:529	This study aimed to determine whether galactinol, a key limiting factor in RFO biosynthesis, functions as a signal molecule in triggering cold tolerance.
31616940	0	9	theme	cold	92:95	arg1	stress					97:102	cold stress	92:102	cold stress	92:102	Expression of galactinol synthase from Ammopiptanthus nanus in tomato improves tolerance to cold stress.
31616940	6	10	theme	sugar	926:930	arg1	composition					932:942	the sugar composition	922:942	the sugar composition of the seeds and seedlings	922:969	Expression of AnGolS1 in tomato enhanced cold tolerance and led to changes in the sugar composition of the seeds and seedlings.
31616940	0	11	from	nanus	54:58	arg1	Expression					0:9	Expression	0:9	Expression of galactinol synthase from Ammopiptanthus nanus in tomato	0:68	Expression of galactinol synthase from Ammopiptanthus nanus in tomato improves tolerance to cold stress.
31616940	0	11	from	nanus	54:58	arg1	synthase					25:32	galactinol synthase	14:32	galactinol synthase from Ammopiptanthus nanus	14:58	Expression of galactinol synthase from Ammopiptanthus nanus in tomato improves tolerance to cold stress.
31616940	2	12	theme	family	276:281	arg1	oligosaccharides					283:298	Raffinose family oligosaccharides	266:298	Raffinose family oligosaccharides (RFOs)	266:305	Raffinose family oligosaccharides (RFOs) are an example and play an important role in abiotic stress tolerance.
31616940	2	12	theme	family	276:281	arg1	example					314:320	an example	311:320	an example	311:320	Raffinose family oligosaccharides (RFOs) are an example and play an important role in abiotic stress tolerance.
31616940	2	12	theme	family	276:281	arg1	RFOs					301:304	RFOs	301:304	RFOs	301:304	Raffinose family oligosaccharides (RFOs) are an example and play an important role in abiotic stress tolerance.
31616940	8	13	theme	pathway	1139:1145	arg1	repression					1108:1117	the repression	1104:1117	the repression of the ET signaling pathway by 1-methylcyclopropene	1104:1169	The application of galactinol abolished the repression of the ET signaling pathway by 1-methylcyclopropene during seed germination.
31616940	6	14	from	Expression	844:853	arg1	tomato					869:874	tomato	869:874	tomato	869:874	Expression of AnGolS1 in tomato enhanced cold tolerance and led to changes in the sugar composition of the seeds and seedlings.
31616940	2	15	theme	Raffinose	266:274	arg1	oligosaccharides					283:298	Raffinose family oligosaccharides	266:298	Raffinose family oligosaccharides (RFOs)	266:305	Raffinose family oligosaccharides (RFOs) are an example and play an important role in abiotic stress tolerance.
31616940	2	15	theme	Raffinose	266:274	arg1	example					314:320	an example	311:320	an example	311:320	Raffinose family oligosaccharides (RFOs) are an example and play an important role in abiotic stress tolerance.
31616940	2	15	theme	Raffinose	266:274	arg1	RFOs					301:304	RFOs	301:304	RFOs	301:304	Raffinose family oligosaccharides (RFOs) are an example and play an important role in abiotic stress tolerance.
31616940	8	16	theme	signaling	1129:1137	arg1	pathway					1139:1145	the ET signaling pathway	1122:1145	the ET signaling pathway	1122:1145	The application of galactinol abolished the repression of the ET signaling pathway by 1-methylcyclopropene during seed germination.
31616940	4	17	theme	°C.	705:707	arg1	AnGolS1					709:715	-30 °C to 47 °C. AnGolS1	692:715	-30 °C to 47 °C. AnGolS1	692:715	Exposure to low temperatures induces the expression of galactinol synthase (AnGolS1) in Ammopiptanthus nanus, a desert plant that survives temperatures between -30 °C to 47 °C. AnGolS1 has a greater catalytic activity than tomato galactinol synthase (SlGolS2).
31616940	6	18	dep	seeds	951:955	arg1	the					947:949	the	947:949	the	947:949	Expression of AnGolS1 in tomato enhanced cold tolerance and led to changes in the sugar composition of the seeds and seedlings.
31616940	4	19	theme	desert	644:649	arg1	plant					651:655	a desert plant	642:655	a desert plant that survives temperatures between -30 °C to 47 °C. AnGolS1	642:715	Exposure to low temperatures induces the expression of galactinol synthase (AnGolS1) in Ammopiptanthus nanus, a desert plant that survives temperatures between -30 °C to 47 °C. AnGolS1 has a greater catalytic activity than tomato galactinol synthase (SlGolS2).
31616940	8	20	theme	ET	1126:1127	arg1	pathway					1139:1145	the ET signaling pathway	1122:1145	the ET signaling pathway	1122:1145	The application of galactinol abolished the repression of the ET signaling pathway by 1-methylcyclopropene during seed germination.
31616940	7	21	theme	ethylene	1039:1046	arg1	signaling					1053:1061	ethylene (ET) signaling	1039:1061	ethylene (ET) signaling	1039:1061	AnGolS1 transgenic tomato lines exhibited an enhanced capacity for ethylene (ET) signaling.
31616940	4	22	theme	47	702:703	arg1	°C					696:697	°C	696:697	°C	696:697	Exposure to low temperatures induces the expression of galactinol synthase (AnGolS1) in Ammopiptanthus nanus, a desert plant that survives temperatures between -30 °C to 47 °C. AnGolS1 has a greater catalytic activity than tomato galactinol synthase (SlGolS2).
31616940	2	23	theme	stress	360:365	arg1	tolerance					367:375	abiotic stress tolerance	352:375	abiotic stress tolerance	352:375	Raffinose family oligosaccharides (RFOs) are an example and play an important role in abiotic stress tolerance.
31616940	0	24	theme	synthase	25:32	arg1	Expression					0:9	Expression	0:9	Expression of galactinol synthase from Ammopiptanthus nanus in tomato	0:68	Expression of galactinol synthase from Ammopiptanthus nanus in tomato improves tolerance to cold stress.
31616940	4	25	contain	has	717:719	arg2	activity					741:748	a greater catalytic activity	721:748	a greater catalytic activity than tomato galactinol synthase (SlGolS2)	721:790	Exposure to low temperatures induces the expression of galactinol synthase (AnGolS1) in Ammopiptanthus nanus, a desert plant that survives temperatures between -30 °C to 47 °C. AnGolS1 has a greater catalytic activity than tomato galactinol synthase (SlGolS2).
31616940	4	25	contain	has	717:719	arg1	plant					651:655	a desert plant	642:655	a desert plant that survives temperatures between -30 °C to 47 °C. AnGolS1	642:715	Exposure to low temperatures induces the expression of galactinol synthase (AnGolS1) in Ammopiptanthus nanus, a desert plant that survives temperatures between -30 °C to 47 °C. AnGolS1 has a greater catalytic activity than tomato galactinol synthase (SlGolS2).
31616940	6	26	from	changes	911:917	arg1	composition					932:942	the sugar composition	922:942	the sugar composition of the seeds and seedlings	922:969	Expression of AnGolS1 in tomato enhanced cold tolerance and led to changes in the sugar composition of the seeds and seedlings.
31616940	0	27	theme	galactinol	14:23	arg1	synthase					25:32	galactinol synthase	14:32	galactinol synthase from Ammopiptanthus nanus	14:58	Expression of galactinol synthase from Ammopiptanthus nanus in tomato improves tolerance to cold stress.
31616940	4	28	theme	low	544:546	arg1	temperatures					548:559	low temperatures	544:559	low temperatures	544:559	Exposure to low temperatures induces the expression of galactinol synthase (AnGolS1) in Ammopiptanthus nanus, a desert plant that survives temperatures between -30 °C to 47 °C. AnGolS1 has a greater catalytic activity than tomato galactinol synthase (SlGolS2).
31616940	2	29	theme	abiotic	352:358	arg1	tolerance					367:375	abiotic stress tolerance	352:375	abiotic stress tolerance	352:375	Raffinose family oligosaccharides (RFOs) are an example and play an important role in abiotic stress tolerance.
31616940	1	30	theme	Soluble	105:111	arg1	carbohydrates					113:125	Soluble carbohydrates	105:125	Soluble carbohydrates	105:125	Soluble carbohydrates not only directly affect plant growth and development but also act as signal molecules in processes that enhance tolerance to cold stress.
31616940	1	30	theme	Soluble	105:111	arg1	molecules					204:212	signal molecules	197:212	signal molecules in processes that enhance tolerance to cold stress	197:263	Soluble carbohydrates not only directly affect plant growth and development but also act as signal molecules in processes that enhance tolerance to cold stress.
31616940	1	31	theme	signal	197:202	arg1	carbohydrates					113:125	Soluble carbohydrates	105:125	Soluble carbohydrates	105:125	Soluble carbohydrates not only directly affect plant growth and development but also act as signal molecules in processes that enhance tolerance to cold stress.
31616940	1	31	theme	signal	197:202	arg1	molecules					204:212	signal molecules	197:212	signal molecules in processes that enhance tolerance to cold stress	197:263	Soluble carbohydrates not only directly affect plant growth and development but also act as signal molecules in processes that enhance tolerance to cold stress.
31616940	9	32	theme	factors	1245:1251	arg1	expression					1213:1222	the expression	1209:1222	the expression of ERF transcription factors	1209:1251	In addition, the expression of ERF transcription factors was increased.
31616940	5	33	theme	low	832:834	arg1	levels					836:841	low levels	832:841	low levels	832:841	Moreover, SlGolS2 is expressed only at low levels.
31616940	3	34	theme	key	430:432	arg1	factor					443:448	a key limiting factor	428:448	a key limiting factor in RFO biosynthesis	428:468	This study aimed to determine whether galactinol, a key limiting factor in RFO biosynthesis, functions as a signal molecule in triggering cold tolerance.
31616940	3	34	theme	key	430:432	arg1	galactinol					416:425	galactinol	416:425	galactinol	416:425	This study aimed to determine whether galactinol, a key limiting factor in RFO biosynthesis, functions as a signal molecule in triggering cold tolerance.
31616940	7	35	dep	ethylene	1039:1046	arg1	ET					1049:1050	ET	1049:1050	ET	1049:1050	AnGolS1 transgenic tomato lines exhibited an enhanced capacity for ethylene (ET) signaling.
31616940	6	36	theme	seeds	951:955	arg1	composition					932:942	the sugar composition	922:942	the sugar composition of the seeds and seedlings	922:969	Expression of AnGolS1 in tomato enhanced cold tolerance and led to changes in the sugar composition of the seeds and seedlings.
31616940	1	37	from	molecules	204:212	arg1	processes					217:225	processes	217:225	processes that enhance tolerance to cold stress	217:263	Soluble carbohydrates not only directly affect plant growth and development but also act as signal molecules in processes that enhance tolerance to cold stress.
31616940	3	38	theme	limiting	434:441	arg1	factor					443:448	a key limiting factor	428:448	a key limiting factor in RFO biosynthesis	428:468	This study aimed to determine whether galactinol, a key limiting factor in RFO biosynthesis, functions as a signal molecule in triggering cold tolerance.
31616940	3	38	theme	limiting	434:441	arg1	galactinol					416:425	galactinol	416:425	galactinol	416:425	This study aimed to determine whether galactinol, a key limiting factor in RFO biosynthesis, functions as a signal molecule in triggering cold tolerance.
31616940	7	39	theme	tomato	991:996	arg1	lines					998:1002	AnGolS1 transgenic tomato lines	972:1002	AnGolS1 transgenic tomato lines	972:1002	AnGolS1 transgenic tomato lines exhibited an enhanced capacity for ethylene (ET) signaling.
31616940	4	40	theme	-30	692:694	arg1	°C					696:697	°C	696:697	°C	696:697	Exposure to low temperatures induces the expression of galactinol synthase (AnGolS1) in Ammopiptanthus nanus, a desert plant that survives temperatures between -30 °C to 47 °C. AnGolS1 has a greater catalytic activity than tomato galactinol synthase (SlGolS2).
31616940	6	41	theme	AnGolS1	858:864	arg1	Expression					844:853	Expression	844:853	Expression of AnGolS1 in tomato	844:874	Expression of AnGolS1 in tomato enhanced cold tolerance and led to changes in the sugar composition of the seeds and seedlings.
31616940	7	42	theme	enhanced	1017:1024	arg1	capacity					1026:1033	an enhanced capacity	1014:1033	an enhanced capacity for ethylene (ET) signaling	1014:1061	AnGolS1 transgenic tomato lines exhibited an enhanced capacity for ethylene (ET) signaling.
31616940	3	43	from	factor	443:448	arg1	biosynthesis					457:468	RFO biosynthesis	453:468	RFO biosynthesis	453:468	This study aimed to determine whether galactinol, a key limiting factor in RFO biosynthesis, functions as a signal molecule in triggering cold tolerance.
31616940	6	44	theme	cold	885:888	arg1	tolerance					890:898	cold tolerance	885:898	cold tolerance	885:898	Expression of AnGolS1 in tomato enhanced cold tolerance and led to changes in the sugar composition of the seeds and seedlings.
31616940	7	45	theme	AnGolS1	972:978	arg1	lines					998:1002	AnGolS1 transgenic tomato lines	972:1002	AnGolS1 transgenic tomato lines	972:1002	AnGolS1 transgenic tomato lines exhibited an enhanced capacity for ethylene (ET) signaling.
31616940	4	46	theme	tomato	755:760	arg1	SlGolS2					783:789	SlGolS2	783:789	SlGolS2	783:789	Exposure to low temperatures induces the expression of galactinol synthase (AnGolS1) in Ammopiptanthus nanus, a desert plant that survives temperatures between -30 °C to 47 °C. AnGolS1 has a greater catalytic activity than tomato galactinol synthase (SlGolS2).
31616940	4	46	theme	tomato	755:760	arg1	synthase					773:780	tomato galactinol synthase	755:780	tomato galactinol synthase (SlGolS2)	755:790	Exposure to low temperatures induces the expression of galactinol synthase (AnGolS1) in Ammopiptanthus nanus, a desert plant that survives temperatures between -30 °C to 47 °C. AnGolS1 has a greater catalytic activity than tomato galactinol synthase (SlGolS2).
31616940	3	47	theme	RFO	453:455	arg1	biosynthesis					457:468	RFO biosynthesis	453:468	RFO biosynthesis	453:468	This study aimed to determine whether galactinol, a key limiting factor in RFO biosynthesis, functions as a signal molecule in triggering cold tolerance.
31616940	4	48	theme	galactinol	587:596	arg1	AnGolS1					608:614	AnGolS1	608:614	AnGolS1	608:614	Exposure to low temperatures induces the expression of galactinol synthase (AnGolS1) in Ammopiptanthus nanus, a desert plant that survives temperatures between -30 °C to 47 °C. AnGolS1 has a greater catalytic activity than tomato galactinol synthase (SlGolS2).
31616940	4	48	theme	galactinol	587:596	arg1	synthase					598:605	galactinol synthase	587:605	galactinol synthase (AnGolS1)	587:615	Exposure to low temperatures induces the expression of galactinol synthase (AnGolS1) in Ammopiptanthus nanus, a desert plant that survives temperatures between -30 °C to 47 °C. AnGolS1 has a greater catalytic activity than tomato galactinol synthase (SlGolS2).
31616940	4	49	theme	catalytic	731:739	arg1	activity					741:748	a greater catalytic activity	721:748	a greater catalytic activity than tomato galactinol synthase (SlGolS2)	721:790	Exposure to low temperatures induces the expression of galactinol synthase (AnGolS1) in Ammopiptanthus nanus, a desert plant that survives temperatures between -30 °C to 47 °C. AnGolS1 has a greater catalytic activity than tomato galactinol synthase (SlGolS2).
31616940	4	50	theme	greater	723:729	arg1	activity					741:748	a greater catalytic activity	721:748	a greater catalytic activity than tomato galactinol synthase (SlGolS2)	721:790	Exposure to low temperatures induces the expression of galactinol synthase (AnGolS1) in Ammopiptanthus nanus, a desert plant that survives temperatures between -30 °C to 47 °C. AnGolS1 has a greater catalytic activity than tomato galactinol synthase (SlGolS2).
31616940	10	51	theme	ET	1332:1333	arg1	pathway					1335:1341	the ET pathway	1328:1341	the ET pathway	1328:1341	Galactinol may therefore act as a signal molecule affecting the ET pathway.
31616940	10	52	theme	signal	1302:1307	arg1	Galactinol					1268:1277	Galactinol	1268:1277	Galactinol	1268:1277	Galactinol may therefore act as a signal molecule affecting the ET pathway.
31616940	10	52	theme	signal	1302:1307	arg1	molecule					1309:1316	a signal molecule	1300:1316	a signal molecule affecting the ET pathway	1300:1341	Galactinol may therefore act as a signal molecule affecting the ET pathway.
31616940	6	53	theme	seedlings	961:969	arg1	composition					932:942	the sugar composition	922:942	the sugar composition of the seeds and seedlings	922:969	Expression of AnGolS1 in tomato enhanced cold tolerance and led to changes in the sugar composition of the seeds and seedlings.
31616940	9	54	theme	ERF	1227:1229	arg1	factors					1245:1251	ERF transcription factors	1227:1251	ERF transcription factors	1227:1251	In addition, the expression of ERF transcription factors was increased.
31616940	3	55	theme	signal	486:491	arg1	galactinol					416:425	galactinol	416:425	galactinol	416:425	This study aimed to determine whether galactinol, a key limiting factor in RFO biosynthesis, functions as a signal molecule in triggering cold tolerance.
31616940	3	55	theme	signal	486:491	arg1	molecule					493:500	a signal molecule	484:500	a signal molecule	484:500	This study aimed to determine whether galactinol, a key limiting factor in RFO biosynthesis, functions as a signal molecule in triggering cold tolerance.
31616940	9	56	theme	transcription	1231:1243	arg1	factors					1245:1251	ERF transcription factors	1227:1251	ERF transcription factors	1227:1251	In addition, the expression of ERF transcription factors was increased.
31616940	4	57	theme	galactinol	762:771	arg1	SlGolS2					783:789	SlGolS2	783:789	SlGolS2	783:789	Exposure to low temperatures induces the expression of galactinol synthase (AnGolS1) in Ammopiptanthus nanus, a desert plant that survives temperatures between -30 °C to 47 °C. AnGolS1 has a greater catalytic activity than tomato galactinol synthase (SlGolS2).
31616940	4	57	theme	galactinol	762:771	arg1	synthase					773:780	tomato galactinol synthase	755:780	tomato galactinol synthase (SlGolS2)	755:790	Exposure to low temperatures induces the expression of galactinol synthase (AnGolS1) in Ammopiptanthus nanus, a desert plant that survives temperatures between -30 °C to 47 °C. AnGolS1 has a greater catalytic activity than tomato galactinol synthase (SlGolS2).
31616940	1	58	theme	plant	152:156	arg1	growth					158:163	plant growth	152:163	plant growth	152:163	Soluble carbohydrates not only directly affect plant growth and development but also act as signal molecules in processes that enhance tolerance to cold stress.
31616940	4	59	theme	synthase	598:605	arg1	expression					573:582	the expression	569:582	the expression of galactinol synthase (AnGolS1) in Ammopiptanthus nanus	569:639	Exposure to low temperatures induces the expression of galactinol synthase (AnGolS1) in Ammopiptanthus nanus, a desert plant that survives temperatures between -30 °C to 47 °C. AnGolS1 has a greater catalytic activity than tomato galactinol synthase (SlGolS2).
34217782	11	0	dep	-norketamine	1446:1457	arg1	R					1444:1444	R	1444:1444	R	1444:1444	Furthermore, (S)-norketamine, but not (R)-norketamine, significantly attenuated the increased levels of Bacteroides caecigallinarum.
34217782	6	1	from	increase	969:976	arg1	levels					1067:1072	the levels	1063:1072	the levels of Harryflintia	1063:1088	At the genus level, (S)-norketamine, but not (R)-norketamine, significantly attenuated the increase in the levels of Escherichia-Shigella and Adlercreutzia, as well as the reduction in the levels of Harryflintia.
34217782	6	1	from	increase	969:976	arg1	levels					985:990	the levels	981:990	the levels of Escherichia-Shigella and Adlercreutzia	981:1032	At the genus level, (S)-norketamine, but not (R)-norketamine, significantly attenuated the increase in the levels of Escherichia-Shigella and Adlercreutzia, as well as the reduction in the levels of Harryflintia.
34217782	1	2	theme	glutamatergic	140:152	arg1	NMDAR					185:189	NMDAR	185:189	NMDAR	185:189	The non-competitive glutamatergic N-methyl-d-aspartate receptor (NMDAR) antagonist, (R, S)-ketamine (ketamine), is known to exert rapid and long-lasting antidepressant-like effects.
34217782	1	2	theme	glutamatergic	140:152	arg1	receptor					175:182	glutamatergic N-methyl-d-aspartate receptor	140:182	The non-competitive glutamatergic N-methyl-d-aspartate receptor (NMDAR) antagonist	120:201	The non-competitive glutamatergic N-methyl-d-aspartate receptor (NMDAR) antagonist, (R, S)-ketamine (ketamine), is known to exert rapid and long-lasting antidepressant-like effects.
34217782	0	3	theme	S	65:65	arg1	-norketamine					67:78	(S)-norketamine	64:78	(S)-norketamine	64:78	Gut microbiota is involved in the antidepressant-like effect of (S)-norketamine in an inflammation model of depression.
34217782	1	4	theme	N-methyl-d-aspartate	154:173	arg1	NMDAR					185:189	NMDAR	185:189	NMDAR	185:189	The non-competitive glutamatergic N-methyl-d-aspartate receptor (NMDAR) antagonist, (R, S)-ketamine (ketamine), is known to exert rapid and long-lasting antidepressant-like effects.
34217782	1	4	theme	N-methyl-d-aspartate	154:173	arg1	receptor					175:182	glutamatergic N-methyl-d-aspartate receptor	140:182	The non-competitive glutamatergic N-methyl-d-aspartate receptor (NMDAR) antagonist	120:201	The non-competitive glutamatergic N-methyl-d-aspartate receptor (NMDAR) antagonist, (R, S)-ketamine (ketamine), is known to exert rapid and long-lasting antidepressant-like effects.
34217782	6	5	dep	-norketamine	926:937	arg1	R					924:924	R	924:924	R	924:924	At the genus level, (S)-norketamine, but not (R)-norketamine, significantly attenuated the increase in the levels of Escherichia-Shigella and Adlercreutzia, as well as the reduction in the levels of Harryflintia.
34217782	13	6	theme	depression	1790:1799	arg1	treatment					1801:1809	depression treatment	1790:1809	depression treatment	1790:1809	Therapeutic strategies improving the gut microbiota might facilitate the benefits for depression treatment.
34217782	9	7	theme	bacterium	1351:1359	arg1	levels					1341:1346	the levels	1337:1346	the levels of bacterium ic1379 and Bacteroides sp	1337:1385	Notably, (S)-norketamine was more potent than (R)-norketamine at reducing the levels of bacterium ic1379 and Bacteroides sp.
34217782	5	8	theme	-induced	863:870	arg1	mice					872:875	the lipopolysaccharide (LPS)-induced mice	835:875	the lipopolysaccharide (LPS)-induced mice	835:875	In the behavioral tests, (S)-norketamine, but not (R)-norketamine, showed antidepressant-like effects in the lipopolysaccharide (LPS)-induced mice.
34217782	12	9	from	changes	1658:1664	arg1	composition					1673:1683	the composition	1669:1683	the composition of gut microbiota	1669:1701	In conclusion, this study suggests that the antidepressant-like effects of (S)-norketamine might be associated with the changes in the composition of gut microbiota.
34217782	0	10	theme	-norketamine	67:78	arg1	effect					54:59	the antidepressant-like effect	30:59	the antidepressant-like effect of (S)-norketamine in an inflammation model of depression	30:117	Gut microbiota is involved in the antidepressant-like effect of (S)-norketamine in an inflammation model of depression.
34217782	0	11	from	effect	54:59	arg1	model					99:103	an inflammation model	83:103	an inflammation model of depression	83:117	Gut microbiota is involved in the antidepressant-like effect of (S)-norketamine in an inflammation model of depression.
34217782	6	12	theme	genus	885:889	arg1	level					891:895	the genus level	881:895	the genus level	881:895	At the genus level, (S)-norketamine, but not (R)-norketamine, significantly attenuated the increase in the levels of Escherichia-Shigella and Adlercreutzia, as well as the reduction in the levels of Harryflintia.
34217782	12	13	theme	antidepressant-like	1582:1600	arg1	effects					1602:1608	the antidepressant-like effects	1578:1608	the antidepressant-like effects of (S)-norketamine	1578:1627	In conclusion, this study suggests that the antidepressant-like effects of (S)-norketamine might be associated with the changes in the composition of gut microbiota.
34217782	3	14	theme	particular	502:511	arg1	-enantiomer					520:530	particular the (S)-enantiomer	502:530	particular the (S)-enantiomer	502:530	Very recently, we demonstrated that a major metabolite of ketamine, norketamine, in particular the (S)-enantiomer, had a potent antidepressant-like effect.
34217782	7	15	from	increase	1183:1190	arg1	levels					1199:1204	the levels	1195:1204	the levels of bacterium ic1379 and Bacteroides sp	1195:1243	At the species level, both (S)-norketamine and (R)-norketamine significantly attenuated the increase in the levels of bacterium ic1379 and Bacteroides sp.
34217782	3	16	contain	had	533:535	arg1	norketamine					486:496	norketamine	486:496	norketamine	486:496	Very recently, we demonstrated that a major metabolite of ketamine, norketamine, in particular the (S)-enantiomer, had a potent antidepressant-like effect.
34217782	3	16	contain	had	533:535	arg2	effect					566:571	a potent antidepressant-like effect	537:571	a potent antidepressant-like effect	537:571	Very recently, we demonstrated that a major metabolite of ketamine, norketamine, in particular the (S)-enantiomer, had a potent antidepressant-like effect.
34217782	3	16	contain	had	533:535	arg1	metabolite					462:471	a major metabolite	454:471	a major metabolite of ketamine	454:483	Very recently, we demonstrated that a major metabolite of ketamine, norketamine, in particular the (S)-enantiomer, had a potent antidepressant-like effect.
34217782	0	17	theme	inflammation	86:97	arg1	model					99:103	an inflammation model	83:103	an inflammation model of depression	83:117	Gut microbiota is involved in the antidepressant-like effect of (S)-norketamine in an inflammation model of depression.
34217782	9	18	theme	R	1310:1310	arg1	-norketamine					1312:1323	(R)-norketamine	1309:1323	(R)-norketamine	1309:1323	Notably, (S)-norketamine was more potent than (R)-norketamine at reducing the levels of bacterium ic1379 and Bacteroides sp.
34217782	12	19	theme	microbiota	1692:1701	arg1	composition					1673:1683	the composition	1669:1683	the composition of gut microbiota	1669:1701	In conclusion, this study suggests that the antidepressant-like effects of (S)-norketamine might be associated with the changes in the composition of gut microbiota.
34217782	11	20	theme	-norketamine	1446:1457	arg1	-norketamine					1421:1432	(S)-norketamine	1418:1432	(S)-norketamine	1418:1432	Furthermore, (S)-norketamine, but not (R)-norketamine, significantly attenuated the increased levels of Bacteroides caecigallinarum.
34217782	4	21	theme	microbiota	718:727	arg1	composition					699:709	the composition	695:709	the composition of gut microbiota	695:727	We here examined the effects of a low-dose of norketamine enantiomers on depression symptoms and detected the changes in the composition of gut microbiota.
34217782	6	22	theme	-norketamine	926:937	arg1	-norketamine					901:912	(S)-norketamine	898:912	(S)-norketamine	898:912	At the genus level, (S)-norketamine, but not (R)-norketamine, significantly attenuated the increase in the levels of Escherichia-Shigella and Adlercreutzia, as well as the reduction in the levels of Harryflintia.
34217782	1	23	theme	receptor	175:182	arg1	-ketamine					210:218	-ketamine	210:218	(R, S)-ketamine (ketamine)	204:229	The non-competitive glutamatergic N-methyl-d-aspartate receptor (NMDAR) antagonist, (R, S)-ketamine (ketamine), is known to exert rapid and long-lasting antidepressant-like effects.
34217782	1	23	theme	receptor	175:182	arg1	antagonist					192:201	The non-competitive glutamatergic N-methyl-d-aspartate receptor (NMDAR) antagonist	120:201	The non-competitive glutamatergic N-methyl-d-aspartate receptor (NMDAR) antagonist	120:201	The non-competitive glutamatergic N-methyl-d-aspartate receptor (NMDAR) antagonist, (R, S)-ketamine (ketamine), is known to exert rapid and long-lasting antidepressant-like effects.
34217782	5	24	theme	behavioral	737:746	arg1	tests					748:752	the behavioral tests	733:752	the behavioral tests	733:752	In the behavioral tests, (S)-norketamine, but not (R)-norketamine, showed antidepressant-like effects in the lipopolysaccharide (LPS)-induced mice.
34217782	5	25	theme	antidepressant-like	804:822	arg1	effects					824:830	antidepressant-like effects	804:830	antidepressant-like effects	804:830	In the behavioral tests, (S)-norketamine, but not (R)-norketamine, showed antidepressant-like effects in the lipopolysaccharide (LPS)-induced mice.
34217782	12	26	theme	gut	1688:1690	arg1	microbiota					1692:1701	gut microbiota	1688:1701	gut microbiota	1688:1701	In conclusion, this study suggests that the antidepressant-like effects of (S)-norketamine might be associated with the changes in the composition of gut microbiota.
34217782	4	27	theme	gut	714:716	arg1	microbiota					718:727	gut microbiota	714:727	gut microbiota	714:727	We here examined the effects of a low-dose of norketamine enantiomers on depression symptoms and detected the changes in the composition of gut microbiota.
34217782	6	28	from	reduction	1050:1058	arg1	levels					1067:1072	the levels	1063:1072	the levels of Harryflintia	1063:1088	At the genus level, (S)-norketamine, but not (R)-norketamine, significantly attenuated the increase in the levels of Escherichia-Shigella and Adlercreutzia, as well as the reduction in the levels of Harryflintia.
34217782	6	28	from	reduction	1050:1058	arg1	levels					985:990	the levels	981:990	the levels of Escherichia-Shigella and Adlercreutzia	981:1032	At the genus level, (S)-norketamine, but not (R)-norketamine, significantly attenuated the increase in the levels of Escherichia-Shigella and Adlercreutzia, as well as the reduction in the levels of Harryflintia.
34217782	3	29	theme	S	518:518	arg1	-enantiomer					520:530	particular the (S)-enantiomer	502:530	particular the (S)-enantiomer	502:530	Very recently, we demonstrated that a major metabolite of ketamine, norketamine, in particular the (S)-enantiomer, had a potent antidepressant-like effect.
34217782	4	30	from	effects	595:601	arg1	symptoms					658:665	depression symptoms	647:665	depression symptoms	647:665	We here examined the effects of a low-dose of norketamine enantiomers on depression symptoms and detected the changes in the composition of gut microbiota.
34217782	4	31	theme	low-dose	608:615	arg1	effects					595:601	the effects	591:601	the effects of a low-dose of norketamine enantiomers on depression symptoms	591:665	We here examined the effects of a low-dose of norketamine enantiomers on depression symptoms and detected the changes in the composition of gut microbiota.
34217782	0	32	theme	Gut	0:2	arg1	microbiota					4:13	Gut microbiota	0:13	Gut microbiota	0:13	Gut microbiota is involved in the antidepressant-like effect of (S)-norketamine in an inflammation model of depression.
34217782	7	33	theme	species	1098:1104	arg1	level					1106:1110	the species level	1094:1110	the species level	1094:1110	At the species level, both (S)-norketamine and (R)-norketamine significantly attenuated the increase in the levels of bacterium ic1379 and Bacteroides sp.
34217782	9	34	theme	sp	1384:1385	arg1	levels					1341:1346	the levels	1337:1346	the levels of bacterium ic1379 and Bacteroides sp	1337:1385	Notably, (S)-norketamine was more potent than (R)-norketamine at reducing the levels of bacterium ic1379 and Bacteroides sp.
34217782	4	35	from	changes	684:690	arg1	composition					699:709	the composition	695:709	the composition of gut microbiota	695:727	We here examined the effects of a low-dose of norketamine enantiomers on depression symptoms and detected the changes in the composition of gut microbiota.
34217782	2	36	theme	ketamine	329:336	arg1	restricted					341:350	restricted	341:350	restricted	341:350	However, the widely use of ketamine is restricted owing to severe psychotomimetic side-effects and abuse liability.
34217782	2	36	theme	ketamine	329:336	arg1	use					322:324	the widely use	311:324	the widely use of ketamine	311:336	However, the widely use of ketamine is restricted owing to severe psychotomimetic side-effects and abuse liability.
34217782	2	37	theme	abuse	401:405	arg1	liability					407:415	abuse liability	401:415	abuse liability	401:415	However, the widely use of ketamine is restricted owing to severe psychotomimetic side-effects and abuse liability.
34217782	7	38	theme	bacterium	1209:1217	arg1	levels					1199:1204	the levels	1195:1204	the levels of bacterium ic1379 and Bacteroides sp	1195:1243	At the species level, both (S)-norketamine and (R)-norketamine significantly attenuated the increase in the levels of bacterium ic1379 and Bacteroides sp.
34217782	5	39	dep	-norketamine	783:794	arg1	R					781:781	R	781:781	R	781:781	In the behavioral tests, (S)-norketamine, but not (R)-norketamine, showed antidepressant-like effects in the lipopolysaccharide (LPS)-induced mice.
34217782	7	40	theme	R	1139:1139	arg1	-norketamine					1141:1152	(R)-norketamine	1138:1152	(R)-norketamine	1138:1152	At the species level, both (S)-norketamine and (R)-norketamine significantly attenuated the increase in the levels of bacterium ic1379 and Bacteroides sp.
34217782	5	41	theme	S	756:756	arg1	-norketamine					758:769	(S)-norketamine	755:769	(S)-norketamine	755:769	In the behavioral tests, (S)-norketamine, but not (R)-norketamine, showed antidepressant-like effects in the lipopolysaccharide (LPS)-induced mice.
34217782	4	42	theme	enantiomers	632:642	arg1	low-dose					608:615	low-dose	608:615	low-dose	608:615	We here examined the effects of a low-dose of norketamine enantiomers on depression symptoms and detected the changes in the composition of gut microbiota.
34217782	2	43	theme	psychotomimetic	368:382	arg1	side-effects					384:395	severe psychotomimetic side-effects	361:395	severe psychotomimetic side-effects	361:395	However, the widely use of ketamine is restricted owing to severe psychotomimetic side-effects and abuse liability.
34217782	4	44	theme	depression	647:656	arg1	symptoms					658:665	depression symptoms	647:665	depression symptoms	647:665	We here examined the effects of a low-dose of norketamine enantiomers on depression symptoms and detected the changes in the composition of gut microbiota.
34217782	1	45	dep	-ketamine	210:218	arg1	S					208:208	S	208:208	S	208:208	The non-competitive glutamatergic N-methyl-d-aspartate receptor (NMDAR) antagonist, (R, S)-ketamine (ketamine), is known to exert rapid and long-lasting antidepressant-like effects.
34217782	1	45	dep	-ketamine	210:218	arg1	R					205:205	R	205:205	R	205:205	The non-competitive glutamatergic N-methyl-d-aspartate receptor (NMDAR) antagonist, (R, S)-ketamine (ketamine), is known to exert rapid and long-lasting antidepressant-like effects.
34217782	7	46	theme	Bacteroides	1230:1240	arg1	sp					1242:1243	bacterium ic1379 and Bacteroides sp	1209:1243	sp	1242:1243	At the species level, both (S)-norketamine and (R)-norketamine significantly attenuated the increase in the levels of bacterium ic1379 and Bacteroides sp.
34217782	2	47	theme	severe	361:366	arg1	side-effects					384:395	severe psychotomimetic side-effects	361:395	severe psychotomimetic side-effects	361:395	However, the widely use of ketamine is restricted owing to severe psychotomimetic side-effects and abuse liability.
34217782	4	48	theme	norketamine	620:630	arg1	enantiomers					632:642	norketamine enantiomers	620:642	norketamine enantiomers	620:642	We here examined the effects of a low-dose of norketamine enantiomers on depression symptoms and detected the changes in the composition of gut microbiota.
34217782	1	49	theme	rapid	250:254	arg1	effects					293:299	rapid and long-lasting antidepressant-like effects	250:299	rapid and long-lasting antidepressant-like effects	250:299	The non-competitive glutamatergic N-methyl-d-aspartate receptor (NMDAR) antagonist, (R, S)-ketamine (ketamine), is known to exert rapid and long-lasting antidepressant-like effects.
34217782	0	50	theme	depression	108:117	arg1	model					99:103	an inflammation model	83:103	an inflammation model of depression	83:117	Gut microbiota is involved in the antidepressant-like effect of (S)-norketamine in an inflammation model of depression.
34217782	12	51	theme	-norketamine	1616:1627	arg1	effects					1602:1608	the antidepressant-like effects	1578:1608	the antidepressant-like effects of (S)-norketamine	1578:1627	In conclusion, this study suggests that the antidepressant-like effects of (S)-norketamine might be associated with the changes in the composition of gut microbiota.
34217782	13	52	theme	Therapeutic	1704:1714	arg1	strategies					1716:1725	Therapeutic strategies	1704:1725	Therapeutic strategies improving the gut microbiota	1704:1754	Therapeutic strategies improving the gut microbiota might facilitate the benefits for depression treatment.
34217782	5	53	theme	-norketamine	783:794	arg1	-norketamine					758:769	(S)-norketamine	755:769	(S)-norketamine	755:769	In the behavioral tests, (S)-norketamine, but not (R)-norketamine, showed antidepressant-like effects in the lipopolysaccharide (LPS)-induced mice.
34217782	11	54	theme	increased	1489:1497	arg1	levels					1499:1504	the increased levels	1485:1504	the increased levels of Bacteroides caecigallinarum	1485:1535	Furthermore, (S)-norketamine, but not (R)-norketamine, significantly attenuated the increased levels of Bacteroides caecigallinarum.
34217782	9	55	theme	S	1273:1273	arg1	-norketamine					1275:1286	(S)-norketamine	1272:1286	(S)-norketamine	1272:1286	Notably, (S)-norketamine was more potent than (R)-norketamine at reducing the levels of bacterium ic1379 and Bacteroides sp.
34217782	6	56	theme	Escherichia-Shigella	995:1014	arg1	levels					985:990	the levels	981:990	the levels of Escherichia-Shigella and Adlercreutzia	981:1032	At the genus level, (S)-norketamine, but not (R)-norketamine, significantly attenuated the increase in the levels of Escherichia-Shigella and Adlercreutzia, as well as the reduction in the levels of Harryflintia.
34217782	0	57	theme	antidepressant-like	34:52	arg1	effect					54:59	the antidepressant-like effect	30:59	the antidepressant-like effect of (S)-norketamine in an inflammation model of depression	30:117	Gut microbiota is involved in the antidepressant-like effect of (S)-norketamine in an inflammation model of depression.
34217782	1	58	theme	long-lasting	260:271	arg1	effects					293:299	rapid and long-lasting antidepressant-like effects	250:299	rapid and long-lasting antidepressant-like effects	250:299	The non-competitive glutamatergic N-methyl-d-aspartate receptor (NMDAR) antagonist, (R, S)-ketamine (ketamine), is known to exert rapid and long-lasting antidepressant-like effects.
34217782	6	59	theme	Harryflintia	1077:1088	arg1	levels					1067:1072	the levels	1063:1072	the levels of Harryflintia	1063:1088	At the genus level, (S)-norketamine, but not (R)-norketamine, significantly attenuated the increase in the levels of Escherichia-Shigella and Adlercreutzia, as well as the reduction in the levels of Harryflintia.
34217782	3	60	theme	major	456:460	arg1	norketamine					486:496	norketamine	486:496	norketamine	486:496	Very recently, we demonstrated that a major metabolite of ketamine, norketamine, in particular the (S)-enantiomer, had a potent antidepressant-like effect.
34217782	3	60	theme	major	456:460	arg1	metabolite					462:471	a major metabolite	454:471	a major metabolite of ketamine	454:483	Very recently, we demonstrated that a major metabolite of ketamine, norketamine, in particular the (S)-enantiomer, had a potent antidepressant-like effect.
34217782	1	61	theme	antidepressant-like	273:291	arg1	effects					293:299	rapid and long-lasting antidepressant-like effects	250:299	rapid and long-lasting antidepressant-like effects	250:299	The non-competitive glutamatergic N-methyl-d-aspartate receptor (NMDAR) antagonist, (R, S)-ketamine (ketamine), is known to exert rapid and long-lasting antidepressant-like effects.
34217782	6	62	theme	Adlercreutzia	1020:1032	arg1	levels					985:990	the levels	981:990	the levels of Escherichia-Shigella and Adlercreutzia	981:1032	At the genus level, (S)-norketamine, but not (R)-norketamine, significantly attenuated the increase in the levels of Escherichia-Shigella and Adlercreutzia, as well as the reduction in the levels of Harryflintia.
34217782	7	63	theme	sp	1242:1243	arg1	levels					1199:1204	the levels	1195:1204	the levels of bacterium ic1379 and Bacteroides sp	1195:1243	At the species level, both (S)-norketamine and (R)-norketamine significantly attenuated the increase in the levels of bacterium ic1379 and Bacteroides sp.
34217782	9	64	theme	Bacteroides	1372:1382	arg1	sp					1384:1385	bacterium ic1379 and Bacteroides sp	1351:1385	sp	1384:1385	Notably, (S)-norketamine was more potent than (R)-norketamine at reducing the levels of bacterium ic1379 and Bacteroides sp.
34217782	11	65	theme	Bacteroides	1509:1519	arg1	caecigallinarum					1521:1535	Bacteroides caecigallinarum	1509:1535	Bacteroides caecigallinarum	1509:1535	Furthermore, (S)-norketamine, but not (R)-norketamine, significantly attenuated the increased levels of Bacteroides caecigallinarum.
34217782	7	66	theme	S	1119:1119	arg1	-norketamine					1121:1132	(S)-norketamine	1118:1132	(S)-norketamine	1118:1132	At the species level, both (S)-norketamine and (R)-norketamine significantly attenuated the increase in the levels of bacterium ic1379 and Bacteroides sp.
34217782	11	67	theme	caecigallinarum	1521:1535	arg1	levels					1499:1504	the increased levels	1485:1504	the increased levels of Bacteroides caecigallinarum	1485:1535	Furthermore, (S)-norketamine, but not (R)-norketamine, significantly attenuated the increased levels of Bacteroides caecigallinarum.
34217782	3	68	theme	potent	539:544	arg1	effect					566:571	a potent antidepressant-like effect	537:571	a potent antidepressant-like effect	537:571	Very recently, we demonstrated that a major metabolite of ketamine, norketamine, in particular the (S)-enantiomer, had a potent antidepressant-like effect.
34217782	3	69	theme	ketamine	476:483	arg1	norketamine					486:496	norketamine	486:496	norketamine	486:496	Very recently, we demonstrated that a major metabolite of ketamine, norketamine, in particular the (S)-enantiomer, had a potent antidepressant-like effect.
34217782	3	69	theme	ketamine	476:483	arg1	metabolite					462:471	a major metabolite	454:471	a major metabolite of ketamine	454:483	Very recently, we demonstrated that a major metabolite of ketamine, norketamine, in particular the (S)-enantiomer, had a potent antidepressant-like effect.
34217782	13	70	theme	gut	1741:1743	arg1	microbiota					1745:1754	the gut microbiota	1737:1754	the gut microbiota	1737:1754	Therapeutic strategies improving the gut microbiota might facilitate the benefits for depression treatment.
34217782	11	71	theme	S	1419:1419	arg1	-norketamine					1421:1432	(S)-norketamine	1418:1432	(S)-norketamine	1418:1432	Furthermore, (S)-norketamine, but not (R)-norketamine, significantly attenuated the increased levels of Bacteroides caecigallinarum.
34217782	3	72	theme	antidepressant-like	546:564	arg1	effect					566:571	a potent antidepressant-like effect	537:571	a potent antidepressant-like effect	537:571	Very recently, we demonstrated that a major metabolite of ketamine, norketamine, in particular the (S)-enantiomer, had a potent antidepressant-like effect.
34217782	12	73	theme	S	1614:1614	arg1	-norketamine					1616:1627	(S)-norketamine	1613:1627	(S)-norketamine	1613:1627	In conclusion, this study suggests that the antidepressant-like effects of (S)-norketamine might be associated with the changes in the composition of gut microbiota.
34217782	1	74	theme	non-competitive	124:138	arg1	-ketamine					210:218	-ketamine	210:218	(R, S)-ketamine (ketamine)	204:229	The non-competitive glutamatergic N-methyl-d-aspartate receptor (NMDAR) antagonist, (R, S)-ketamine (ketamine), is known to exert rapid and long-lasting antidepressant-like effects.
34217782	1	74	theme	non-competitive	124:138	arg1	antagonist					192:201	The non-competitive glutamatergic N-methyl-d-aspartate receptor (NMDAR) antagonist	120:201	The non-competitive glutamatergic N-methyl-d-aspartate receptor (NMDAR) antagonist	120:201	The non-competitive glutamatergic N-methyl-d-aspartate receptor (NMDAR) antagonist, (R, S)-ketamine (ketamine), is known to exert rapid and long-lasting antidepressant-like effects.
34217782	3	75	from	norketamine	486:496	arg1	-enantiomer					520:530	particular the (S)-enantiomer	502:530	particular the (S)-enantiomer	502:530	Very recently, we demonstrated that a major metabolite of ketamine, norketamine, in particular the (S)-enantiomer, had a potent antidepressant-like effect.
34217782	6	76	theme	S	899:899	arg1	-norketamine					901:912	(S)-norketamine	898:912	(S)-norketamine	898:912	At the genus level, (S)-norketamine, but not (R)-norketamine, significantly attenuated the increase in the levels of Escherichia-Shigella and Adlercreutzia, as well as the reduction in the levels of Harryflintia.
32373113	8	0	theme	regulatory	1573:1582	arg1	role					1584:1587	its regulatory role	1569:1587	its regulatory role for B cell-mediated immunity	1569:1616	This study provides the first comprehensive characterization of MIM in B cells, demonstrates its regulatory role for B cell-mediated immunity, as well as proposes new functions for MIM in tuning receptor signaling and cellular metabolism, processes, which may also contribute to the poorly understood functions of MIM in cancer.
32373113	3	1	theme	cytoskeleton	676:687	arg1	action					633:638	coordinated action	621:638	coordinated action of cellular membranes and the actin cytoskeleton, tightly controlled by concerted action of multiple regulatory proteins, some of them exclusive to B cells	621:794	BCR signaling and a number of steps downstream of it rely on coordinated action of cellular membranes and the actin cytoskeleton, tightly controlled by concerted action of multiple regulatory proteins, some of them exclusive to B cells.
32373113	8	2	theme	MIM	1790:1792	arg1	functions					1777:1785	the poorly understood functions	1755:1785	the poorly understood functions of MIM in cancer	1755:1802	This study provides the first comprehensive characterization of MIM in B cells, demonstrates its regulatory role for B cell-mediated immunity, as well as proposes new functions for MIM in tuning receptor signaling and cellular metabolism, processes, which may also contribute to the poorly understood functions of MIM in cancer.
32373113	7	3	theme	antibody	1408:1415	arg1	responses					1417:1425	impaired IgM antibody responses	1395:1425	impaired IgM antibody responses to immunization with T cell-independent antigen	1395:1473	In vivo, MIM knockout animals exhibit impaired IgM antibody responses to immunization with T cell-independent antigen.
32373113	4	4	theme	knockout	1018:1025	arg1	strain					1033:1038	MIM knockout mouse strain	1014:1038	MIM knockout mouse strain	1014:1038	Here, we dissect the role of Missing-In-Metastasis (MIM), or Metastasis suppressor 1 (MTSS1), a cancer-associated membrane and actin cytoskeleton regulating protein, in B cell-mediated immunity by taking advantage of MIM knockout mouse strain.
32373113	7	5	with	immunization	1430:1441	arg1	antigen					1467:1473	T cell-independent antigen	1448:1473	T cell-independent antigen	1448:1473	In vivo, MIM knockout animals exhibit impaired IgM antibody responses to immunization with T cell-independent antigen.
32373113	7	6	theme	impaired	1395:1402	arg1	responses					1417:1425	impaired IgM antibody responses	1395:1425	impaired IgM antibody responses to immunization with T cell-independent antigen	1395:1473	In vivo, MIM knockout animals exhibit impaired IgM antibody responses to immunization with T cell-independent antigen.
32373113	2	7	theme	cognate	271:277	arg1	antigens					279:286	cognate antigens	271:286	cognate antigens	271:286	B cell activation by cognate antigens via B cell receptors (BCRs), or pathogen-associated molecules through pattern-recognition receptors, such as Toll-like receptors (TLRs), leads to transcriptional and metabolic changes that ultimately transform B cells into antibody-producing plasma cells or memory cells.
32373113	1	8	theme	B	190:190	arg1	cells					192:196	B cells	190:196	B cells	190:196	Efficient generation of antibodies by B cells is one of the prerequisites of protective immunity.
32373113	4	9	theme	Missing-In-Metastasis	826:846	arg1	role					818:821	the role	814:821	the role of Missing-In-Metastasis (MIM), or Metastasis suppressor 1 (MTSS1), a cancer-associated membrane and actin cytoskeleton regulating protein, in B cell-mediated immunity	814:989	Here, we dissect the role of Missing-In-Metastasis (MIM), or Metastasis suppressor 1 (MTSS1), a cancer-associated membrane and actin cytoskeleton regulating protein, in B cell-mediated immunity by taking advantage of MIM knockout mouse strain.
32373113	3	10	theme	proteins	752:759	arg1	action					722:727	concerted action	712:727	concerted action of multiple regulatory proteins, some of them exclusive to B cells	712:794	BCR signaling and a number of steps downstream of it rely on coordinated action of cellular membranes and the actin cytoskeleton, tightly controlled by concerted action of multiple regulatory proteins, some of them exclusive to B cells.
32373113	8	11	from	characterization	1520:1535	arg1	cells					1549:1553	B cells	1547:1553	B cells	1547:1553	This study provides the first comprehensive characterization of MIM in B cells, demonstrates its regulatory role for B cell-mediated immunity, as well as proposes new functions for MIM in tuning receptor signaling and cellular metabolism, processes, which may also contribute to the poorly understood functions of MIM in cancer.
32373113	8	12	theme	tuning	1664:1669	arg1	signaling					1680:1688	tuning receptor signaling	1664:1688	tuning receptor signaling	1664:1688	This study provides the first comprehensive characterization of MIM in B cells, demonstrates its regulatory role for B cell-mediated immunity, as well as proposes new functions for MIM in tuning receptor signaling and cellular metabolism, processes, which may also contribute to the poorly understood functions of MIM in cancer.
32373113	3	13	theme	multiple	732:739	arg1	proteins					752:759	multiple regulatory proteins	732:759	multiple regulatory proteins	732:759	BCR signaling and a number of steps downstream of it rely on coordinated action of cellular membranes and the actin cytoskeleton, tightly controlled by concerted action of multiple regulatory proteins, some of them exclusive to B cells.
32373113	3	13	theme	multiple	732:739	arg1	them					770:773	them	770:773	them	770:773	BCR signaling and a number of steps downstream of it rely on coordinated action of cellular membranes and the actin cytoskeleton, tightly controlled by concerted action of multiple regulatory proteins, some of them exclusive to B cells.
32373113	3	13	theme	multiple	732:739	arg1	some					762:765	some	762:765	some	762:765	BCR signaling and a number of steps downstream of it rely on coordinated action of cellular membranes and the actin cytoskeleton, tightly controlled by concerted action of multiple regulatory proteins, some of them exclusive to B cells.
32373113	0	14	theme	Cell-Independent	117:132	arg1	Responses					141:149	T Cell-Independent Immune Responses	115:149	T Cell-Independent Immune Responses	115:149	Missing-in-Metastasis/Metastasis Suppressor 1 Regulates B Cell Receptor Signaling, B Cell Metabolic Potential, and T Cell-Independent Immune Responses.
32373113	2	15	theme	cell	294:297	arg1	receptors					299:307	B cell receptors	292:307	B cell receptors (BCRs)	292:314	B cell activation by cognate antigens via B cell receptors (BCRs), or pathogen-associated molecules through pattern-recognition receptors, such as Toll-like receptors (TLRs), leads to transcriptional and metabolic changes that ultimately transform B cells into antibody-producing plasma cells or memory cells.
32373113	2	15	theme	cell	294:297	arg1	BCRs					310:313	BCRs	310:313	BCRs	310:313	B cell activation by cognate antigens via B cell receptors (BCRs), or pathogen-associated molecules through pattern-recognition receptors, such as Toll-like receptors (TLRs), leads to transcriptional and metabolic changes that ultimately transform B cells into antibody-producing plasma cells or memory cells.
32373113	2	16	theme	memory	546:551	arg1	cells					553:557	memory cells	546:557	memory cells	546:557	B cell activation by cognate antigens via B cell receptors (BCRs), or pathogen-associated molecules through pattern-recognition receptors, such as Toll-like receptors (TLRs), leads to transcriptional and metabolic changes that ultimately transform B cells into antibody-producing plasma cells or memory cells.
32373113	3	17	theme	downstream	596:605	arg1	steps					590:594	steps	590:594	steps downstream of it	590:611	BCR signaling and a number of steps downstream of it rely on coordinated action of cellular membranes and the actin cytoskeleton, tightly controlled by concerted action of multiple regulatory proteins, some of them exclusive to B cells.
32373113	8	18	theme	cellular	1694:1701	arg1	metabolism					1703:1712	cellular metabolism	1694:1712	cellular metabolism	1694:1712	This study provides the first comprehensive characterization of MIM in B cells, demonstrates its regulatory role for B cell-mediated immunity, as well as proposes new functions for MIM in tuning receptor signaling and cellular metabolism, processes, which may also contribute to the poorly understood functions of MIM in cancer.
32373113	1	19	theme	prerequisites	212:224	arg1	immunity					240:247	protective immunity	229:247	protective immunity	229:247	Efficient generation of antibodies by B cells is one of the prerequisites of protective immunity.
32373113	1	19	theme	prerequisites	212:224	arg1	one					201:203	one	201:203	one	201:203	Efficient generation of antibodies by B cells is one of the prerequisites of protective immunity.
32373113	1	19	theme	prerequisites	212:224	arg1	prerequisites					212:224	the prerequisites	208:224	the prerequisites of protective immunity	208:247	Efficient generation of antibodies by B cells is one of the prerequisites of protective immunity.
32373113	4	20	theme	Metastasis	858:867	arg1	suppressor					869:878	Metastasis suppressor 1	858:880	Metastasis suppressor 1 (MTSS1)	858:888	Here, we dissect the role of Missing-In-Metastasis (MIM), or Metastasis suppressor 1 (MTSS1), a cancer-associated membrane and actin cytoskeleton regulating protein, in B cell-mediated immunity by taking advantage of MIM knockout mouse strain.
32373113	4	20	theme	Metastasis	858:867	arg1	MTSS1					883:887	MTSS1	883:887	MTSS1	883:887	Here, we dissect the role of Missing-In-Metastasis (MIM), or Metastasis suppressor 1 (MTSS1), a cancer-associated membrane and actin cytoskeleton regulating protein, in B cell-mediated immunity by taking advantage of MIM knockout mouse strain.
32373113	6	21	theme	MIM-/-	1181:1186	arg1	cells					1190:1194	MIM-/- B cells	1181:1194	MIM-/- B cells	1181:1194	Interestingly, we found that MIM-/- B cells are defected in BCR signaling in response to surface-bound antigens but, on the other hand, show increased metabolic activity after stimulation with LPS or CpG.
32373113	6	22	theme	surface-bound	1241:1253	arg1	antigens					1255:1262	surface-bound antigens	1241:1262	surface-bound antigens	1241:1262	Interestingly, we found that MIM-/- B cells are defected in BCR signaling in response to surface-bound antigens but, on the other hand, show increased metabolic activity after stimulation with LPS or CpG.
32373113	3	23	theme	it	610:611	arg1	downstream					596:605	downstream	596:605	downstream	596:605	BCR signaling and a number of steps downstream of it rely on coordinated action of cellular membranes and the actin cytoskeleton, tightly controlled by concerted action of multiple regulatory proteins, some of them exclusive to B cells.
32373113	0	24	theme	Cell	85:88	arg1	Potential					100:108	B Cell Metabolic Potential	83:108	B Cell Metabolic Potential	83:108	Missing-in-Metastasis/Metastasis Suppressor 1 Regulates B Cell Receptor Signaling, B Cell Metabolic Potential, and T Cell-Independent Immune Responses.
32373113	5	25	theme	normal	1092:1097	arg1	composition					1099:1109	largely normal composition	1084:1109	largely normal composition of B cell compartments	1084:1132	We show undisturbed B cell development and largely normal composition of B cell compartments in the periphery.
32373113	1	26	theme	antibodies	176:185	arg1	generation					162:171	Efficient generation	152:171	Efficient generation of antibodies by B cells	152:196	Efficient generation of antibodies by B cells is one of the prerequisites of protective immunity.
32373113	4	27	theme	cancer-associated	893:909	arg1	membrane					911:918	a cancer-associated membrane	891:918	a cancer-associated membrane	891:918	Here, we dissect the role of Missing-In-Metastasis (MIM), or Metastasis suppressor 1 (MTSS1), a cancer-associated membrane and actin cytoskeleton regulating protein, in B cell-mediated immunity by taking advantage of MIM knockout mouse strain.
32373113	4	27	theme	cancer-associated	893:909	arg1	Missing-In-Metastasis					826:846	Missing-In-Metastasis	826:846	Missing-In-Metastasis (MIM)	826:852	Here, we dissect the role of Missing-In-Metastasis (MIM), or Metastasis suppressor 1 (MTSS1), a cancer-associated membrane and actin cytoskeleton regulating protein, in B cell-mediated immunity by taking advantage of MIM knockout mouse strain.
32373113	6	28	theme	increased	1293:1301	arg1	activity					1313:1320	increased metabolic activity	1293:1320	increased metabolic activity	1293:1320	Interestingly, we found that MIM-/- B cells are defected in BCR signaling in response to surface-bound antigens but, on the other hand, show increased metabolic activity after stimulation with LPS or CpG.
32373113	5	29	theme	undisturbed	1049:1059	arg1	development					1068:1078	undisturbed B cell development	1049:1078	undisturbed B cell development	1049:1078	We show undisturbed B cell development and largely normal composition of B cell compartments in the periphery.
32373113	2	30	theme	antibody-producing	511:528	arg1	cells					537:541	antibody-producing plasma cells	511:541	antibody-producing plasma cells	511:541	B cell activation by cognate antigens via B cell receptors (BCRs), or pathogen-associated molecules through pattern-recognition receptors, such as Toll-like receptors (TLRs), leads to transcriptional and metabolic changes that ultimately transform B cells into antibody-producing plasma cells or memory cells.
32373113	2	31	theme	Toll-like	397:405	arg1	TLRs					418:421	TLRs	418:421	TLRs	418:421	B cell activation by cognate antigens via B cell receptors (BCRs), or pathogen-associated molecules through pattern-recognition receptors, such as Toll-like receptors (TLRs), leads to transcriptional and metabolic changes that ultimately transform B cells into antibody-producing plasma cells or memory cells.
32373113	2	31	theme	Toll-like	397:405	arg1	receptors					407:415	Toll-like receptors	397:415	Toll-like receptors (TLRs)	397:422	B cell activation by cognate antigens via B cell receptors (BCRs), or pathogen-associated molecules through pattern-recognition receptors, such as Toll-like receptors (TLRs), leads to transcriptional and metabolic changes that ultimately transform B cells into antibody-producing plasma cells or memory cells.
32373113	4	32	from	role	818:821	arg1	immunity					982:989	B cell-mediated immunity	966:989	B cell-mediated immunity	966:989	Here, we dissect the role of Missing-In-Metastasis (MIM), or Metastasis suppressor 1 (MTSS1), a cancer-associated membrane and actin cytoskeleton regulating protein, in B cell-mediated immunity by taking advantage of MIM knockout mouse strain.
32373113	8	33	theme	understood	1766:1775	arg1	functions					1777:1785	the poorly understood functions	1755:1785	the poorly understood functions of MIM in cancer	1755:1802	This study provides the first comprehensive characterization of MIM in B cells, demonstrates its regulatory role for B cell-mediated immunity, as well as proposes new functions for MIM in tuning receptor signaling and cellular metabolism, processes, which may also contribute to the poorly understood functions of MIM in cancer.
32373113	2	34	theme	transcriptional	434:448	arg1	changes					464:470	transcriptional and metabolic changes	434:470	transcriptional and metabolic changes that ultimately transform B cells into antibody-producing plasma cells or memory cells	434:557	B cell activation by cognate antigens via B cell receptors (BCRs), or pathogen-associated molecules through pattern-recognition receptors, such as Toll-like receptors (TLRs), leads to transcriptional and metabolic changes that ultimately transform B cells into antibody-producing plasma cells or memory cells.
32373113	8	35	from	MIM	1790:1792	arg1	cancer					1797:1802	cancer	1797:1802	cancer	1797:1802	This study provides the first comprehensive characterization of MIM in B cells, demonstrates its regulatory role for B cell-mediated immunity, as well as proposes new functions for MIM in tuning receptor signaling and cellular metabolism, processes, which may also contribute to the poorly understood functions of MIM in cancer.
32373113	0	36	theme	Missing-in-Metastasis/Metastasis	0:31	arg1	Suppressor					33:42	Missing-in-Metastasis/Metastasis Suppressor 1	0:44	Missing-in-Metastasis/Metastasis Suppressor 1	0:44	Missing-in-Metastasis/Metastasis Suppressor 1 Regulates B Cell Receptor Signaling, B Cell Metabolic Potential, and T Cell-Independent Immune Responses.
32373113	2	37	theme	B	250:250	arg1	activation					257:266	B cell activation	250:266	B cell activation by cognate antigens via B cell receptors (BCRs), or pathogen-associated molecules through pattern-recognition receptors, such as Toll-like receptors (TLRs),	250:423	B cell activation by cognate antigens via B cell receptors (BCRs), or pathogen-associated molecules through pattern-recognition receptors, such as Toll-like receptors (TLRs), leads to transcriptional and metabolic changes that ultimately transform B cells into antibody-producing plasma cells or memory cells.
32373113	8	38	from	cancer	1797:1802	arg1	functions					1777:1785	the poorly understood functions	1755:1785	the poorly understood functions of MIM in cancer	1755:1802	This study provides the first comprehensive characterization of MIM in B cells, demonstrates its regulatory role for B cell-mediated immunity, as well as proposes new functions for MIM in tuning receptor signaling and cellular metabolism, processes, which may also contribute to the poorly understood functions of MIM in cancer.
32373113	8	39	theme	first	1500:1504	arg1	characterization					1520:1535	the first comprehensive characterization	1496:1535	the first comprehensive characterization of MIM in B cells	1496:1553	This study provides the first comprehensive characterization of MIM in B cells, demonstrates its regulatory role for B cell-mediated immunity, as well as proposes new functions for MIM in tuning receptor signaling and cellular metabolism, processes, which may also contribute to the poorly understood functions of MIM in cancer.
32373113	3	40	theme	cellular	643:650	arg1	membranes					652:660	cellular membranes	643:660	cellular membranes	643:660	BCR signaling and a number of steps downstream of it rely on coordinated action of cellular membranes and the actin cytoskeleton, tightly controlled by concerted action of multiple regulatory proteins, some of them exclusive to B cells.
32373113	0	41	theme	B	56:56	arg1	Signaling					72:80	B Cell Receptor Signaling	56:80	B Cell Receptor Signaling	56:80	Missing-in-Metastasis/Metastasis Suppressor 1 Regulates B Cell Receptor Signaling, B Cell Metabolic Potential, and T Cell-Independent Immune Responses.
32373113	4	42	theme	cell-mediated	968:980	arg1	immunity					982:989	B cell-mediated immunity	966:989	B cell-mediated immunity	966:989	Here, we dissect the role of Missing-In-Metastasis (MIM), or Metastasis suppressor 1 (MTSS1), a cancer-associated membrane and actin cytoskeleton regulating protein, in B cell-mediated immunity by taking advantage of MIM knockout mouse strain.
32373113	2	43	theme	B	498:498	arg1	cells					500:504	B cells	498:504	B cells	498:504	B cell activation by cognate antigens via B cell receptors (BCRs), or pathogen-associated molecules through pattern-recognition receptors, such as Toll-like receptors (TLRs), leads to transcriptional and metabolic changes that ultimately transform B cells into antibody-producing plasma cells or memory cells.
32373113	2	44	theme	pathogen-associated	320:338	arg1	molecules					340:348	pathogen-associated molecules	320:348	pathogen-associated molecules	320:348	B cell activation by cognate antigens via B cell receptors (BCRs), or pathogen-associated molecules through pattern-recognition receptors, such as Toll-like receptors (TLRs), leads to transcriptional and metabolic changes that ultimately transform B cells into antibody-producing plasma cells or memory cells.
32373113	0	45	theme	Receptor	63:70	arg1	Signaling					72:80	B Cell Receptor Signaling	56:80	B Cell Receptor Signaling	56:80	Missing-in-Metastasis/Metastasis Suppressor 1 Regulates B Cell Receptor Signaling, B Cell Metabolic Potential, and T Cell-Independent Immune Responses.
32373113	5	46	theme	cell	1116:1119	arg1	compartments					1121:1132	B cell compartments	1114:1132	B cell compartments	1114:1132	We show undisturbed B cell development and largely normal composition of B cell compartments in the periphery.
32373113	8	47	theme	MIM	1540:1542	arg1	characterization					1520:1535	the first comprehensive characterization	1496:1535	the first comprehensive characterization of MIM in B cells	1496:1553	This study provides the first comprehensive characterization of MIM in B cells, demonstrates its regulatory role for B cell-mediated immunity, as well as proposes new functions for MIM in tuning receptor signaling and cellular metabolism, processes, which may also contribute to the poorly understood functions of MIM in cancer.
32373113	3	48	theme	actin	670:674	arg1	cytoskeleton					676:687	the actin cytoskeleton	666:687	the actin cytoskeleton	666:687	BCR signaling and a number of steps downstream of it rely on coordinated action of cellular membranes and the actin cytoskeleton, tightly controlled by concerted action of multiple regulatory proteins, some of them exclusive to B cells.
32373113	8	49	theme	B	1593:1593	arg1	immunity					1609:1616	B cell-mediated immunity	1593:1616	B cell-mediated immunity	1593:1616	This study provides the first comprehensive characterization of MIM in B cells, demonstrates its regulatory role for B cell-mediated immunity, as well as proposes new functions for MIM in tuning receptor signaling and cellular metabolism, processes, which may also contribute to the poorly understood functions of MIM in cancer.
32373113	8	50	theme	B	1547:1547	arg1	cells					1549:1553	B cells	1547:1553	B cells	1547:1553	This study provides the first comprehensive characterization of MIM in B cells, demonstrates its regulatory role for B cell-mediated immunity, as well as proposes new functions for MIM in tuning receptor signaling and cellular metabolism, processes, which may also contribute to the poorly understood functions of MIM in cancer.
32373113	1	51	theme	immunity	240:247	arg1	immunity					240:247	protective immunity	229:247	protective immunity	229:247	Efficient generation of antibodies by B cells is one of the prerequisites of protective immunity.
32373113	1	51	theme	immunity	240:247	arg1	prerequisites					212:224	the prerequisites	208:224	the prerequisites of protective immunity	208:247	Efficient generation of antibodies by B cells is one of the prerequisites of protective immunity.
32373113	2	52	theme	metabolic	454:462	arg1	changes					464:470	transcriptional and metabolic changes	434:470	transcriptional and metabolic changes that ultimately transform B cells into antibody-producing plasma cells or memory cells	434:557	B cell activation by cognate antigens via B cell receptors (BCRs), or pathogen-associated molecules through pattern-recognition receptors, such as Toll-like receptors (TLRs), leads to transcriptional and metabolic changes that ultimately transform B cells into antibody-producing plasma cells or memory cells.
32373113	4	53	theme	MIM	1014:1016	arg1	strain					1033:1038	MIM knockout mouse strain	1014:1038	MIM knockout mouse strain	1014:1038	Here, we dissect the role of Missing-In-Metastasis (MIM), or Metastasis suppressor 1 (MTSS1), a cancer-associated membrane and actin cytoskeleton regulating protein, in B cell-mediated immunity by taking advantage of MIM knockout mouse strain.
32373113	6	54	from	defected	1200:1207	arg1	signaling					1216:1224	BCR signaling	1212:1224	BCR signaling in response to surface-bound antigens	1212:1262	Interestingly, we found that MIM-/- B cells are defected in BCR signaling in response to surface-bound antigens but, on the other hand, show increased metabolic activity after stimulation with LPS or CpG.
32373113	7	55	theme	IgM	1404:1406	arg1	responses					1417:1425	impaired IgM antibody responses	1395:1425	impaired IgM antibody responses to immunization with T cell-independent antigen	1395:1473	In vivo, MIM knockout animals exhibit impaired IgM antibody responses to immunization with T cell-independent antigen.
32373113	4	56	theme	mouse	1027:1031	arg1	strain					1033:1038	MIM knockout mouse strain	1014:1038	MIM knockout mouse strain	1014:1038	Here, we dissect the role of Missing-In-Metastasis (MIM), or Metastasis suppressor 1 (MTSS1), a cancer-associated membrane and actin cytoskeleton regulating protein, in B cell-mediated immunity by taking advantage of MIM knockout mouse strain.
32373113	2	57	theme	B	292:292	arg1	receptors					299:307	B cell receptors	292:307	B cell receptors (BCRs)	292:314	B cell activation by cognate antigens via B cell receptors (BCRs), or pathogen-associated molecules through pattern-recognition receptors, such as Toll-like receptors (TLRs), leads to transcriptional and metabolic changes that ultimately transform B cells into antibody-producing plasma cells or memory cells.
32373113	2	57	theme	B	292:292	arg1	BCRs					310:313	BCRs	310:313	BCRs	310:313	B cell activation by cognate antigens via B cell receptors (BCRs), or pathogen-associated molecules through pattern-recognition receptors, such as Toll-like receptors (TLRs), leads to transcriptional and metabolic changes that ultimately transform B cells into antibody-producing plasma cells or memory cells.
32373113	7	58	theme	MIM	1366:1368	arg1	animals					1379:1385	MIM knockout animals	1366:1385	MIM knockout animals	1366:1385	In vivo, MIM knockout animals exhibit impaired IgM antibody responses to immunization with T cell-independent antigen.
32373113	6	59	theme	BCR	1212:1214	arg1	signaling					1216:1224	BCR signaling	1212:1224	BCR signaling in response to surface-bound antigens	1212:1262	Interestingly, we found that MIM-/- B cells are defected in BCR signaling in response to surface-bound antigens but, on the other hand, show increased metabolic activity after stimulation with LPS or CpG.
32373113	8	60	theme	new	1639:1641	arg1	functions					1643:1651	new functions	1639:1651	new functions for MIM	1639:1659	This study provides the first comprehensive characterization of MIM in B cells, demonstrates its regulatory role for B cell-mediated immunity, as well as proposes new functions for MIM in tuning receptor signaling and cellular metabolism, processes, which may also contribute to the poorly understood functions of MIM in cancer.
32373113	7	61	theme	knockout	1370:1377	arg1	animals					1379:1385	MIM knockout animals	1366:1385	MIM knockout animals	1366:1385	In vivo, MIM knockout animals exhibit impaired IgM antibody responses to immunization with T cell-independent antigen.
32373113	3	62	theme	concerted	712:720	arg1	action					722:727	concerted action	712:727	concerted action of multiple regulatory proteins, some of them exclusive to B cells	712:794	BCR signaling and a number of steps downstream of it rely on coordinated action of cellular membranes and the actin cytoskeleton, tightly controlled by concerted action of multiple regulatory proteins, some of them exclusive to B cells.
32373113	0	63	theme	Immune	134:139	arg1	Responses					141:149	T Cell-Independent Immune Responses	115:149	T Cell-Independent Immune Responses	115:149	Missing-in-Metastasis/Metastasis Suppressor 1 Regulates B Cell Receptor Signaling, B Cell Metabolic Potential, and T Cell-Independent Immune Responses.
32373113	3	64	theme	BCR	560:562	arg1	signaling					564:572	BCR signaling	560:572	BCR signaling	560:572	BCR signaling and a number of steps downstream of it rely on coordinated action of cellular membranes and the actin cytoskeleton, tightly controlled by concerted action of multiple regulatory proteins, some of them exclusive to B cells.
32373113	3	65	theme	regulatory	741:750	arg1	proteins					752:759	multiple regulatory proteins	732:759	multiple regulatory proteins	732:759	BCR signaling and a number of steps downstream of it rely on coordinated action of cellular membranes and the actin cytoskeleton, tightly controlled by concerted action of multiple regulatory proteins, some of them exclusive to B cells.
32373113	3	65	theme	regulatory	741:750	arg1	them					770:773	them	770:773	them	770:773	BCR signaling and a number of steps downstream of it rely on coordinated action of cellular membranes and the actin cytoskeleton, tightly controlled by concerted action of multiple regulatory proteins, some of them exclusive to B cells.
32373113	3	65	theme	regulatory	741:750	arg1	some					762:765	some	762:765	some	762:765	BCR signaling and a number of steps downstream of it rely on coordinated action of cellular membranes and the actin cytoskeleton, tightly controlled by concerted action of multiple regulatory proteins, some of them exclusive to B cells.
32373113	3	66	theme	steps	590:594	arg1	signaling					564:572	BCR signaling	560:572	BCR signaling	560:572	BCR signaling and a number of steps downstream of it rely on coordinated action of cellular membranes and the actin cytoskeleton, tightly controlled by concerted action of multiple regulatory proteins, some of them exclusive to B cells.
32373113	3	66	theme	steps	590:594	arg1	number					580:585	a number	578:585	a number of steps downstream of it	578:611	BCR signaling and a number of steps downstream of it rely on coordinated action of cellular membranes and the actin cytoskeleton, tightly controlled by concerted action of multiple regulatory proteins, some of them exclusive to B cells.
32373113	8	67	theme	receptor	1671:1678	arg1	signaling					1680:1688	tuning receptor signaling	1664:1688	tuning receptor signaling	1664:1688	This study provides the first comprehensive characterization of MIM in B cells, demonstrates its regulatory role for B cell-mediated immunity, as well as proposes new functions for MIM in tuning receptor signaling and cellular metabolism, processes, which may also contribute to the poorly understood functions of MIM in cancer.
32373113	6	68	theme	B	1188:1188	arg1	cells					1190:1194	MIM-/- B cells	1181:1194	MIM-/- B cells	1181:1194	Interestingly, we found that MIM-/- B cells are defected in BCR signaling in response to surface-bound antigens but, on the other hand, show increased metabolic activity after stimulation with LPS or CpG.
32373113	6	69	with	stimulation	1328:1338	arg1	CpG					1352:1354	CpG	1352:1354	CpG	1352:1354	Interestingly, we found that MIM-/- B cells are defected in BCR signaling in response to surface-bound antigens but, on the other hand, show increased metabolic activity after stimulation with LPS or CpG.
32373113	6	69	with	stimulation	1328:1338	arg1	LPS					1345:1347	LPS	1345:1347	LPS	1345:1347	Interestingly, we found that MIM-/- B cells are defected in BCR signaling in response to surface-bound antigens but, on the other hand, show increased metabolic activity after stimulation with LPS or CpG.
32373113	4	70	theme	suppressor	869:878	arg1	role					818:821	the role	814:821	the role of Missing-In-Metastasis (MIM), or Metastasis suppressor 1 (MTSS1), a cancer-associated membrane and actin cytoskeleton regulating protein, in B cell-mediated immunity	814:989	Here, we dissect the role of Missing-In-Metastasis (MIM), or Metastasis suppressor 1 (MTSS1), a cancer-associated membrane and actin cytoskeleton regulating protein, in B cell-mediated immunity by taking advantage of MIM knockout mouse strain.
32373113	0	71	theme	B	83:83	arg1	Potential					100:108	B Cell Metabolic Potential	83:108	B Cell Metabolic Potential	83:108	Missing-in-Metastasis/Metastasis Suppressor 1 Regulates B Cell Receptor Signaling, B Cell Metabolic Potential, and T Cell-Independent Immune Responses.
32373113	2	72	theme	cell	252:255	arg1	activation					257:266	B cell activation	250:266	B cell activation by cognate antigens via B cell receptors (BCRs), or pathogen-associated molecules through pattern-recognition receptors, such as Toll-like receptors (TLRs),	250:423	B cell activation by cognate antigens via B cell receptors (BCRs), or pathogen-associated molecules through pattern-recognition receptors, such as Toll-like receptors (TLRs), leads to transcriptional and metabolic changes that ultimately transform B cells into antibody-producing plasma cells or memory cells.
32373113	1	73	theme	Efficient	152:160	arg1	generation					162:171	Efficient generation	152:171	Efficient generation of antibodies by B cells	152:196	Efficient generation of antibodies by B cells is one of the prerequisites of protective immunity.
32373113	4	74	theme	actin	924:928	arg1	cytoskeleton					930:941	actin cytoskeleton	924:941	actin cytoskeleton	924:941	Here, we dissect the role of Missing-In-Metastasis (MIM), or Metastasis suppressor 1 (MTSS1), a cancer-associated membrane and actin cytoskeleton regulating protein, in B cell-mediated immunity by taking advantage of MIM knockout mouse strain.
32373113	4	74	theme	actin	924:928	arg1	Missing-In-Metastasis					826:846	Missing-In-Metastasis	826:846	Missing-In-Metastasis (MIM)	826:852	Here, we dissect the role of Missing-In-Metastasis (MIM), or Metastasis suppressor 1 (MTSS1), a cancer-associated membrane and actin cytoskeleton regulating protein, in B cell-mediated immunity by taking advantage of MIM knockout mouse strain.
32373113	8	75	from	functions	1777:1785	arg1	cancer					1797:1802	cancer	1797:1802	cancer	1797:1802	This study provides the first comprehensive characterization of MIM in B cells, demonstrates its regulatory role for B cell-mediated immunity, as well as proposes new functions for MIM in tuning receptor signaling and cellular metabolism, processes, which may also contribute to the poorly understood functions of MIM in cancer.
32373113	3	76	theme	B	788:788	arg1	cells					790:794	B cells	788:794	B cells	788:794	BCR signaling and a number of steps downstream of it rely on coordinated action of cellular membranes and the actin cytoskeleton, tightly controlled by concerted action of multiple regulatory proteins, some of them exclusive to B cells.
32373113	0	77	theme	Metabolic	90:98	arg1	Potential					100:108	B Cell Metabolic Potential	83:108	B Cell Metabolic Potential	83:108	Missing-in-Metastasis/Metastasis Suppressor 1 Regulates B Cell Receptor Signaling, B Cell Metabolic Potential, and T Cell-Independent Immune Responses.
32373113	6	78	theme	other	1276:1280	arg1	hand					1282:1285	the other hand	1272:1285	the other hand	1272:1285	Interestingly, we found that MIM-/- B cells are defected in BCR signaling in response to surface-bound antigens but, on the other hand, show increased metabolic activity after stimulation with LPS or CpG.
32373113	6	79	theme	metabolic	1303:1311	arg1	activity					1313:1320	increased metabolic activity	1293:1320	increased metabolic activity	1293:1320	Interestingly, we found that MIM-/- B cells are defected in BCR signaling in response to surface-bound antigens but, on the other hand, show increased metabolic activity after stimulation with LPS or CpG.
32373113	2	80	theme	plasma	530:535	arg1	cells					537:541	antibody-producing plasma cells	511:541	antibody-producing plasma cells	511:541	B cell activation by cognate antigens via B cell receptors (BCRs), or pathogen-associated molecules through pattern-recognition receptors, such as Toll-like receptors (TLRs), leads to transcriptional and metabolic changes that ultimately transform B cells into antibody-producing plasma cells or memory cells.
32373113	5	81	theme	B	1061:1061	arg1	development					1068:1078	undisturbed B cell development	1049:1078	undisturbed B cell development	1049:1078	We show undisturbed B cell development and largely normal composition of B cell compartments in the periphery.
32373113	1	82	theme	protective	229:238	arg1	immunity					240:247	protective immunity	229:247	protective immunity	229:247	Efficient generation of antibodies by B cells is one of the prerequisites of protective immunity.
32373113	8	83	theme	comprehensive	1506:1518	arg1	characterization					1520:1535	the first comprehensive characterization	1496:1535	the first comprehensive characterization of MIM in B cells	1496:1553	This study provides the first comprehensive characterization of MIM in B cells, demonstrates its regulatory role for B cell-mediated immunity, as well as proposes new functions for MIM in tuning receptor signaling and cellular metabolism, processes, which may also contribute to the poorly understood functions of MIM in cancer.
32373113	0	84	theme	Cell	58:61	arg1	Signaling					72:80	B Cell Receptor Signaling	56:80	B Cell Receptor Signaling	56:80	Missing-in-Metastasis/Metastasis Suppressor 1 Regulates B Cell Receptor Signaling, B Cell Metabolic Potential, and T Cell-Independent Immune Responses.
32373113	3	85	theme	coordinated	621:631	arg1	action					633:638	coordinated action	621:638	coordinated action of cellular membranes and the actin cytoskeleton, tightly controlled by concerted action of multiple regulatory proteins, some of them exclusive to B cells	621:794	BCR signaling and a number of steps downstream of it rely on coordinated action of cellular membranes and the actin cytoskeleton, tightly controlled by concerted action of multiple regulatory proteins, some of them exclusive to B cells.
32373113	5	86	theme	B	1114:1114	arg1	compartments					1121:1132	B cell compartments	1114:1132	B cell compartments	1114:1132	We show undisturbed B cell development and largely normal composition of B cell compartments in the periphery.
32373113	5	87	theme	cell	1063:1066	arg1	development					1068:1078	undisturbed B cell development	1049:1078	undisturbed B cell development	1049:1078	We show undisturbed B cell development and largely normal composition of B cell compartments in the periphery.
32373113	4	88	theme	B	966:966	arg1	immunity					982:989	B cell-mediated immunity	966:989	B cell-mediated immunity	966:989	Here, we dissect the role of Missing-In-Metastasis (MIM), or Metastasis suppressor 1 (MTSS1), a cancer-associated membrane and actin cytoskeleton regulating protein, in B cell-mediated immunity by taking advantage of MIM knockout mouse strain.
32373113	8	89	theme	cell-mediated	1595:1607	arg1	immunity					1609:1616	B cell-mediated immunity	1593:1616	B cell-mediated immunity	1593:1616	This study provides the first comprehensive characterization of MIM in B cells, demonstrates its regulatory role for B cell-mediated immunity, as well as proposes new functions for MIM in tuning receptor signaling and cellular metabolism, processes, which may also contribute to the poorly understood functions of MIM in cancer.
32373113	5	90	theme	compartments	1121:1132	arg1	development					1068:1078	undisturbed B cell development	1049:1078	undisturbed B cell development	1049:1078	We show undisturbed B cell development and largely normal composition of B cell compartments in the periphery.
32373113	5	90	theme	compartments	1121:1132	arg1	composition					1099:1109	largely normal composition	1084:1109	largely normal composition of B cell compartments	1084:1132	We show undisturbed B cell development and largely normal composition of B cell compartments in the periphery.
32373113	7	91	theme	cell-independent	1450:1465	arg1	antigen					1467:1473	T cell-independent antigen	1448:1473	T cell-independent antigen	1448:1473	In vivo, MIM knockout animals exhibit impaired IgM antibody responses to immunization with T cell-independent antigen.
32373113	4	92	theme	strain	1033:1038	arg1	advantage					1001:1009	advantage	1001:1009	advantage of MIM knockout mouse strain	1001:1038	Here, we dissect the role of Missing-In-Metastasis (MIM), or Metastasis suppressor 1 (MTSS1), a cancer-associated membrane and actin cytoskeleton regulating protein, in B cell-mediated immunity by taking advantage of MIM knockout mouse strain.
32373113	3	93	theme	membranes	652:660	arg1	action					633:638	coordinated action	621:638	coordinated action of cellular membranes and the actin cytoskeleton, tightly controlled by concerted action of multiple regulatory proteins, some of them exclusive to B cells	621:794	BCR signaling and a number of steps downstream of it rely on coordinated action of cellular membranes and the actin cytoskeleton, tightly controlled by concerted action of multiple regulatory proteins, some of them exclusive to B cells.
32373113	6	94	from	signaling	1216:1224	arg1	response					1229:1236	response	1229:1236	response to surface-bound antigens	1229:1262	Interestingly, we found that MIM-/- B cells are defected in BCR signaling in response to surface-bound antigens but, on the other hand, show increased metabolic activity after stimulation with LPS or CpG.
32373113	6	94	from	signaling	1216:1224	arg1	defected					1200:1207	defected	1200:1207	defected	1200:1207	Interestingly, we found that MIM-/- B cells are defected in BCR signaling in response to surface-bound antigens but, on the other hand, show increased metabolic activity after stimulation with LPS or CpG.
32373113	6	95	dep	defected	1200:1207	arg1	show					1288:1291	show	1288:1291	show increased metabolic activity after stimulation with LPS or CpG	1288:1354	Interestingly, we found that MIM-/- B cells are defected in BCR signaling in response to surface-bound antigens but, on the other hand, show increased metabolic activity after stimulation with LPS or CpG.
32373113	2	96	theme	pattern-recognition	358:376	arg1	receptors					407:415	Toll-like receptors	397:415	Toll-like receptors (TLRs)	397:422	B cell activation by cognate antigens via B cell receptors (BCRs), or pathogen-associated molecules through pattern-recognition receptors, such as Toll-like receptors (TLRs), leads to transcriptional and metabolic changes that ultimately transform B cells into antibody-producing plasma cells or memory cells.
32373113	2	96	theme	pattern-recognition	358:376	arg1	receptors					378:386	pattern-recognition receptors	358:386	pattern-recognition receptors	358:386	B cell activation by cognate antigens via B cell receptors (BCRs), or pathogen-associated molecules through pattern-recognition receptors, such as Toll-like receptors (TLRs), leads to transcriptional and metabolic changes that ultimately transform B cells into antibody-producing plasma cells or memory cells.
33884622	4	0	from	β-carotene	493:502	arg1	anti-inflammatory					507:523	anti-inflammatory	507:523	anti-inflammatory	507:523	In this research, the properties of β-carotene on anti-inflammatory and the composition of gut microbiota were evaluated in a rat model of UC induced by dextran sulfate sodium (DSS).
33884622	4	1	theme	rat	583:585	arg1	model					587:591	a rat model	581:591	a rat model of UC induced by dextran sulfate sodium (DSS)	581:637	In this research, the properties of β-carotene on anti-inflammatory and the composition of gut microbiota were evaluated in a rat model of UC induced by dextran sulfate sodium (DSS).
33884622	7	2	theme	expression	1056:1065	arg1	levels					1067:1072	the expression levels	1052:1072	the expression levels of phosphorylated p65 (0.60 ± 0.02), p38 (0.57 ± 0.00), Erk (0.63 ± 0.04), and JNK (0.70 ± 0.00)	1052:1169	β-Carotene intervention also lowered the expression levels of phosphorylated p65 (0.60 ± 0.02), p38 (0.57 ± 0.00), Erk (0.63 ± 0.04), and JNK (0.70 ± 0.00).
33884622	13	3	theme	nutritional	1878:1888	arg1	intervention					1890:1901	the nutritional intervention	1874:1901	the nutritional intervention of β-carotene enhancing animal health	1874:1939	PRACTICAL APPLICATION: β-Carotene can relieve ulcerative colitis and regulate the gut microbiota; the nutritional intervention of β-carotene enhancing animal health.
33884622	3	4	theme	microbiota	415:424	arg1	modulation					397:406	the modulation	393:406	the modulation of gut microbiota and UC improvement	393:443	However, the role of β-carotene in the modulation of gut microbiota and UC improvement is unclear.
33884622	2	5	theme	bowel	289:293	arg1	disease					295:301	inflammatory bowel disease	276:301	inflammatory bowel disease	276:301	Ulcerative colitis (UC) is a kind of inflammatory bowel disease that is accompanied by a certain risk of colon cancer.
33884622	13	6	theme	gut	1858:1860	arg1	microbiota					1862:1871	the gut microbiota	1854:1871	the gut microbiota	1854:1871	PRACTICAL APPLICATION: β-Carotene can relieve ulcerative colitis and regulate the gut microbiota; the nutritional intervention of β-carotene enhancing animal health.
33884622	5	7	theme	22.23 ±	805:811	arg1	length					797:802	colon length	791:802	colon length (22.23 ± 0.53%)	791:818	The results revealed that β-carotene significantly (p < 0.05) decreased the severity of colitis in rats, as assessed using body weight (6.00 ± 1.73%), colon length (22.23 ± 0.53%), and disease activity index, and improved the structure of the colon damaged.
33884622	5	7	theme	22.23 ±	805:811	arg1	%					817:817	22.23 ± 0.53%	805:817	22.23 ± 0.53%	805:817	The results revealed that β-carotene significantly (p < 0.05) decreased the severity of colitis in rats, as assessed using body weight (6.00 ± 1.73%), colon length (22.23 ± 0.53%), and disease activity index, and improved the structure of the colon damaged.
33884622	4	8	theme	dextran	610:616	arg1	sulfate					618:624	dextran sulfate sodium (DSS)	610:637	dextran sulfate sodium (DSS)	610:637	In this research, the properties of β-carotene on anti-inflammatory and the composition of gut microbiota were evaluated in a rat model of UC induced by dextran sulfate sodium (DSS).
33884622	8	9	theme	genus	1322:1326	arg1	levels					1328:1333	the phylum and genus levels	1307:1333	the phylum and genus levels of rats	1307:1341	The result of the relative abundance of gut microbiota showed that DSS administration significantly changed the microbial structure at the phylum and genus levels of rats.
33884622	9	10	theme	β-carotene	1357:1366	arg1	treatment					1368:1376	β-carotene treatment	1357:1376	β-carotene treatment	1357:1376	Furthermore, β-carotene treatment significantly increased the abundance of Faecalibacterium, the levels of which negatively correlated with the levels of inflammatory cytokines.
33884622	5	11	theme	disease	825:831	arg1	index					842:846	disease activity index	825:846	disease activity index	825:846	The results revealed that β-carotene significantly (p < 0.05) decreased the severity of colitis in rats, as assessed using body weight (6.00 ± 1.73%), colon length (22.23 ± 0.53%), and disease activity index, and improved the structure of the colon damaged.
33884622	13	12	theme	ulcerative	1822:1831	arg1	colitis					1833:1839	ulcerative colitis	1822:1839	ulcerative colitis	1822:1839	PRACTICAL APPLICATION: β-Carotene can relieve ulcerative colitis and regulate the gut microbiota; the nutritional intervention of β-carotene enhancing animal health.
33884622	1	13	theme	antioxidant	154:164	arg1	activities					188:197	antioxidant and anti-inflammatory activities	154:197	antioxidant and anti-inflammatory activities	154:197	β-Carotene displays antioxidant and anti-inflammatory activities and prevents the development of cancer.
33884622	5	14	theme	colitis	728:734	arg1	severity					716:723	the severity	712:723	the severity of colitis in rats	712:742	The results revealed that β-carotene significantly (p < 0.05) decreased the severity of colitis in rats, as assessed using body weight (6.00 ± 1.73%), colon length (22.23 ± 0.53%), and disease activity index, and improved the structure of the colon damaged.
33884622	1	15	theme	anti-inflammatory	170:186	arg1	activities					188:197	antioxidant and anti-inflammatory activities	154:197	antioxidant and anti-inflammatory activities	154:197	β-Carotene displays antioxidant and anti-inflammatory activities and prevents the development of cancer.
33884622	6	16	theme	colonic	908:914	arg1	levels					916:921	colonic levels	908:921	colonic levels of proinflammatory cytokines	908:950	Moreover, colonic levels of proinflammatory cytokines were significantly lower following β-carotene supplementation.
33884622	0	17	from	regulation	15:24	arg1	sulfate					80:86	dextran sulfate	72:86	dextran sulfate	72:86	Gut microbiota regulation and anti-inflammatory effect of β-carotene in dextran sulfate sodium-stimulated ulcerative colitis in rats.
33884622	8	18	theme	abundance	1199:1207	arg1	result					1176:1181	The result	1172:1181	The result of the relative abundance of gut microbiota	1172:1225	The result of the relative abundance of gut microbiota showed that DSS administration significantly changed the microbial structure at the phylum and genus levels of rats.
33884622	4	19	from	properties	479:488	arg1	anti-inflammatory					507:523	anti-inflammatory	507:523	anti-inflammatory	507:523	In this research, the properties of β-carotene on anti-inflammatory and the composition of gut microbiota were evaluated in a rat model of UC induced by dextran sulfate sodium (DSS).
33884622	4	20	theme	β-carotene	493:502	arg1	composition					533:543	the composition	529:543	the composition of gut microbiota	529:561	In this research, the properties of β-carotene on anti-inflammatory and the composition of gut microbiota were evaluated in a rat model of UC induced by dextran sulfate sodium (DSS).
33884622	4	20	theme	β-carotene	493:502	arg1	properties					479:488	the properties	475:488	the properties of β-carotene on anti-inflammatory	475:523	In this research, the properties of β-carotene on anti-inflammatory and the composition of gut microbiota were evaluated in a rat model of UC induced by dextran sulfate sodium (DSS).
33884622	8	21	theme	gut	1212:1214	arg1	microbiota					1216:1225	gut microbiota	1212:1225	gut microbiota	1212:1225	The result of the relative abundance of gut microbiota showed that DSS administration significantly changed the microbial structure at the phylum and genus levels of rats.
33884622	11	22	theme	microbiota	1673:1682	arg1	regulation					1655:1664	the regulation	1651:1664	the regulation of gut microbiota	1651:1682	β-Carotene can alleviate DSS-induced UC through the regulation of gut microbiota.
33884622	0	23	theme	ulcerative	106:115	arg1	colitis					117:123	ulcerative colitis	106:123	ulcerative colitis in rats	106:131	Gut microbiota regulation and anti-inflammatory effect of β-carotene in dextran sulfate sodium-stimulated ulcerative colitis in rats.
33884622	3	24	from	role	371:374	arg1	modulation					397:406	the modulation	393:406	the modulation of gut microbiota and UC improvement	393:443	However, the role of β-carotene in the modulation of gut microbiota and UC improvement is unclear.
33884622	3	25	theme	UC	430:431	arg1	improvement					433:443	UC improvement	430:443	UC improvement	430:443	However, the role of β-carotene in the modulation of gut microbiota and UC improvement is unclear.
33884622	5	26	theme	colon	883:887	arg1	structure					866:874	the structure	862:874	the structure of the colon damaged	862:895	The results revealed that β-carotene significantly (p < 0.05) decreased the severity of colitis in rats, as assessed using body weight (6.00 ± 1.73%), colon length (22.23 ± 0.53%), and disease activity index, and improved the structure of the colon damaged.
33884622	3	27	theme	β-carotene	379:388	arg1	unclear					448:454	unclear	448:454	unclear	448:454	However, the role of β-carotene in the modulation of gut microbiota and UC improvement is unclear.
33884622	3	27	theme	β-carotene	379:388	arg1	role					371:374	the role	367:374	the role of β-carotene in the modulation of gut microbiota and UC improvement	367:443	However, the role of β-carotene in the modulation of gut microbiota and UC improvement is unclear.
33884622	8	28	theme	DSS	1239:1241	arg1	administration					1243:1256	DSS administration	1239:1256	DSS administration	1239:1256	The result of the relative abundance of gut microbiota showed that DSS administration significantly changed the microbial structure at the phylum and genus levels of rats.
33884622	13	29	theme	PRACTICAL	1776:1784	arg1	APPLICATION					1786:1796	PRACTICAL APPLICATION	1776:1796	PRACTICAL APPLICATION: β-Carotene can relieve ulcerative colitis and regulate the gut microbiota; the nutritional intervention of β-carotene enhancing animal health.	1776:1940	PRACTICAL APPLICATION: β-Carotene can relieve ulcerative colitis and regulate the gut microbiota; the nutritional intervention of β-carotene enhancing animal health.
33884622	10	30	theme	potential	1548:1556	arg1	target					1558:1563	a potential target	1546:1563	a potential target in the alleviation of DSS-induced UC	1546:1600	Faecalibacterium may be a potential target in the alleviation of DSS-induced UC.
33884622	10	30	theme	potential	1548:1556	arg1	Faecalibacterium					1522:1537	Faecalibacterium	1522:1537	Faecalibacterium	1522:1537	Faecalibacterium may be a potential target in the alleviation of DSS-induced UC.
33884622	0	31	theme	Gut	0:2	arg1	regulation					15:24	Gut microbiota regulation	0:24	Gut microbiota regulation	0:24	Gut microbiota regulation and anti-inflammatory effect of β-carotene in dextran sulfate sodium-stimulated ulcerative colitis in rats.
33884622	10	32	theme	DSS-induced	1587:1597	arg1	UC					1599:1600	DSS-induced UC	1587:1600	DSS-induced UC	1587:1600	Faecalibacterium may be a potential target in the alleviation of DSS-induced UC.
33884622	5	33	theme	6.00 ±	776:781	arg1	weight					768:773	body weight	763:773	body weight (6.00 ± 1.73%)	763:788	The results revealed that β-carotene significantly (p < 0.05) decreased the severity of colitis in rats, as assessed using body weight (6.00 ± 1.73%), colon length (22.23 ± 0.53%), and disease activity index, and improved the structure of the colon damaged.
33884622	5	33	theme	6.00 ±	776:781	arg1	%					787:787	6.00 ± 1.73%	776:787	6.00 ± 1.73%	776:787	The results revealed that β-carotene significantly (p < 0.05) decreased the severity of colitis in rats, as assessed using body weight (6.00 ± 1.73%), colon length (22.23 ± 0.53%), and disease activity index, and improved the structure of the colon damaged.
33884622	4	34	theme	microbiota	552:561	arg1	composition					533:543	the composition	529:543	the composition of gut microbiota	529:561	In this research, the properties of β-carotene on anti-inflammatory and the composition of gut microbiota were evaluated in a rat model of UC induced by dextran sulfate sodium (DSS).
33884622	4	34	theme	microbiota	552:561	arg1	properties					479:488	the properties	475:488	the properties of β-carotene on anti-inflammatory	475:523	In this research, the properties of β-carotene on anti-inflammatory and the composition of gut microbiota were evaluated in a rat model of UC induced by dextran sulfate sodium (DSS).
33884622	0	35	theme	anti-inflammatory	30:46	arg1	effect					48:53	anti-inflammatory effect	30:53	anti-inflammatory effect of β-carotene in dextran sulfate	30:86	Gut microbiota regulation and anti-inflammatory effect of β-carotene in dextran sulfate sodium-stimulated ulcerative colitis in rats.
33884622	4	36	dep	sulfate	618:624	arg1	DSS					634:636	DSS	634:636	DSS	634:636	In this research, the properties of β-carotene on anti-inflammatory and the composition of gut microbiota were evaluated in a rat model of UC induced by dextran sulfate sodium (DSS).
33884622	4	36	dep	sulfate	618:624	arg1	sodium					626:631	sodium	626:631	dextran sulfate sodium (DSS)	610:637	In this research, the properties of β-carotene on anti-inflammatory and the composition of gut microbiota were evaluated in a rat model of UC induced by dextran sulfate sodium (DSS).
33884622	12	37	theme	rational	1725:1732	arg1	use					1734:1736	the rational use	1721:1736	the rational use of β-carotene in the treatment of UC	1721:1773	This study provides a reference for the rational use of β-carotene in the treatment of UC.
33884622	6	38	theme	cytokines	942:950	arg1	levels					916:921	colonic levels	908:921	colonic levels of proinflammatory cytokines	908:950	Moreover, colonic levels of proinflammatory cytokines were significantly lower following β-carotene supplementation.
33884622	2	39	theme	cancer	350:355	arg1	risk					336:339	a certain risk	326:339	a certain risk of colon cancer	326:355	Ulcerative colitis (UC) is a kind of inflammatory bowel disease that is accompanied by a certain risk of colon cancer.
33884622	7	40	theme	p65	1092:1094	arg1	levels					1067:1072	the expression levels	1052:1072	the expression levels of phosphorylated p65 (0.60 ± 0.02), p38 (0.57 ± 0.00), Erk (0.63 ± 0.04), and JNK (0.70 ± 0.00)	1052:1169	β-Carotene intervention also lowered the expression levels of phosphorylated p65 (0.60 ± 0.02), p38 (0.57 ± 0.00), Erk (0.63 ± 0.04), and JNK (0.70 ± 0.00).
33884622	12	41	theme	β-carotene	1741:1750	arg1	use					1734:1736	the rational use	1721:1736	the rational use of β-carotene in the treatment of UC	1721:1773	This study provides a reference for the rational use of β-carotene in the treatment of UC.
33884622	9	42	theme	cytokines	1511:1519	arg1	levels					1488:1493	the levels	1484:1493	the levels of inflammatory cytokines	1484:1519	Furthermore, β-carotene treatment significantly increased the abundance of Faecalibacterium, the levels of which negatively correlated with the levels of inflammatory cytokines.
33884622	12	43	from	use	1734:1736	arg1	treatment					1759:1767	the treatment	1755:1767	the treatment of UC	1755:1773	This study provides a reference for the rational use of β-carotene in the treatment of UC.
33884622	2	44	theme	Ulcerative	239:248	arg1	kind					268:271	a kind	266:271	a kind of inflammatory bowel disease that is accompanied by a certain risk of colon cancer	266:355	Ulcerative colitis (UC) is a kind of inflammatory bowel disease that is accompanied by a certain risk of colon cancer.
33884622	2	44	theme	Ulcerative	239:248	arg1	UC					259:260	UC	259:260	UC	259:260	Ulcerative colitis (UC) is a kind of inflammatory bowel disease that is accompanied by a certain risk of colon cancer.
33884622	2	44	theme	Ulcerative	239:248	arg1	colitis					250:256	Ulcerative colitis	239:256	Ulcerative colitis (UC)	239:261	Ulcerative colitis (UC) is a kind of inflammatory bowel disease that is accompanied by a certain risk of colon cancer.
33884622	11	45	theme	DSS-induced	1628:1638	arg1	UC					1640:1641	DSS-induced UC	1628:1641	DSS-induced UC	1628:1641	β-Carotene can alleviate DSS-induced UC through the regulation of gut microbiota.
33884622	13	46	theme	β-carotene	1906:1915	arg1	health					1934:1939	β-carotene enhancing animal health	1906:1939	β-carotene enhancing animal health	1906:1939	PRACTICAL APPLICATION: β-Carotene can relieve ulcerative colitis and regulate the gut microbiota; the nutritional intervention of β-carotene enhancing animal health.
33884622	0	47	theme	β-carotene	58:67	arg1	regulation					15:24	Gut microbiota regulation	0:24	Gut microbiota regulation	0:24	Gut microbiota regulation and anti-inflammatory effect of β-carotene in dextran sulfate sodium-stimulated ulcerative colitis in rats.
33884622	0	47	theme	β-carotene	58:67	arg1	effect					48:53	anti-inflammatory effect	30:53	anti-inflammatory effect of β-carotene in dextran sulfate	30:86	Gut microbiota regulation and anti-inflammatory effect of β-carotene in dextran sulfate sodium-stimulated ulcerative colitis in rats.
33884622	4	48	theme	UC	596:597	arg1	model					587:591	a rat model	581:591	a rat model of UC induced by dextran sulfate sodium (DSS)	581:637	In this research, the properties of β-carotene on anti-inflammatory and the composition of gut microbiota were evaluated in a rat model of UC induced by dextran sulfate sodium (DSS).
33884622	13	49	theme	animal	1927:1932	arg1	health					1934:1939	β-carotene enhancing animal health	1906:1939	β-carotene enhancing animal health	1906:1939	PRACTICAL APPLICATION: β-Carotene can relieve ulcerative colitis and regulate the gut microbiota; the nutritional intervention of β-carotene enhancing animal health.
33884622	2	50	theme	disease	295:301	arg1	kind					268:271	a kind	266:271	a kind of inflammatory bowel disease that is accompanied by a certain risk of colon cancer	266:355	Ulcerative colitis (UC) is a kind of inflammatory bowel disease that is accompanied by a certain risk of colon cancer.
33884622	2	50	theme	disease	295:301	arg1	colitis					250:256	Ulcerative colitis	239:256	Ulcerative colitis (UC)	239:261	Ulcerative colitis (UC) is a kind of inflammatory bowel disease that is accompanied by a certain risk of colon cancer.
33884622	5	51	theme	colon	791:795	arg1	length					797:802	colon length	791:802	colon length (22.23 ± 0.53%)	791:818	The results revealed that β-carotene significantly (p < 0.05) decreased the severity of colitis in rats, as assessed using body weight (6.00 ± 1.73%), colon length (22.23 ± 0.53%), and disease activity index, and improved the structure of the colon damaged.
33884622	5	51	theme	colon	791:795	arg1	%					817:817	22.23 ± 0.53%	805:817	22.23 ± 0.53%	805:817	The results revealed that β-carotene significantly (p < 0.05) decreased the severity of colitis in rats, as assessed using body weight (6.00 ± 1.73%), colon length (22.23 ± 0.53%), and disease activity index, and improved the structure of the colon damaged.
33884622	4	52	from	anti-inflammatory	507:523	arg1	composition					533:543	the composition	529:543	the composition of gut microbiota	529:561	In this research, the properties of β-carotene on anti-inflammatory and the composition of gut microbiota were evaluated in a rat model of UC induced by dextran sulfate sodium (DSS).
33884622	4	52	from	anti-inflammatory	507:523	arg1	properties					479:488	the properties	475:488	the properties of β-carotene on anti-inflammatory	475:523	In this research, the properties of β-carotene on anti-inflammatory and the composition of gut microbiota were evaluated in a rat model of UC induced by dextran sulfate sodium (DSS).
33884622	7	53	theme	β-Carotene	1015:1024	arg1	intervention					1026:1037	β-Carotene intervention	1015:1037	β-Carotene intervention	1015:1037	β-Carotene intervention also lowered the expression levels of phosphorylated p65 (0.60 ± 0.02), p38 (0.57 ± 0.00), Erk (0.63 ± 0.04), and JNK (0.70 ± 0.00).
33884622	2	54	theme	certain	328:334	arg1	risk					336:339	a certain risk	326:339	a certain risk of colon cancer	326:355	Ulcerative colitis (UC) is a kind of inflammatory bowel disease that is accompanied by a certain risk of colon cancer.
33884622	13	55	dep	APPLICATION	1786:1796	arg1	regulate					1845:1852	regulate	1845:1852	regulate the gut microbiota	1845:1871	PRACTICAL APPLICATION: β-Carotene can relieve ulcerative colitis and regulate the gut microbiota; the nutritional intervention of β-carotene enhancing animal health.
33884622	13	55	dep	APPLICATION	1786:1796	arg1	relieve					1814:1820	relieve	1814:1820	relieve ulcerative colitis	1814:1839	PRACTICAL APPLICATION: β-Carotene can relieve ulcerative colitis and regulate the gut microbiota; the nutritional intervention of β-carotene enhancing animal health.
33884622	1	56	theme	cancer	231:236	arg1	development					216:226	the development	212:226	the development of cancer	212:236	β-Carotene displays antioxidant and anti-inflammatory activities and prevents the development of cancer.
33884622	5	57	from	severity	716:723	arg1	rats					739:742	rats	739:742	rats	739:742	The results revealed that β-carotene significantly (p < 0.05) decreased the severity of colitis in rats, as assessed using body weight (6.00 ± 1.73%), colon length (22.23 ± 0.53%), and disease activity index, and improved the structure of the colon damaged.
33884622	8	58	theme	rats	1338:1341	arg1	levels					1328:1333	the phylum and genus levels	1307:1333	the phylum and genus levels of rats	1307:1341	The result of the relative abundance of gut microbiota showed that DSS administration significantly changed the microbial structure at the phylum and genus levels of rats.
33884622	2	59	theme	inflammatory	276:287	arg1	disease					295:301	inflammatory bowel disease	276:301	inflammatory bowel disease	276:301	Ulcerative colitis (UC) is a kind of inflammatory bowel disease that is accompanied by a certain risk of colon cancer.
33884622	5	60	theme	activity	833:840	arg1	index					842:846	disease activity index	825:846	disease activity index	825:846	The results revealed that β-carotene significantly (p < 0.05) decreased the severity of colitis in rats, as assessed using body weight (6.00 ± 1.73%), colon length (22.23 ± 0.53%), and disease activity index, and improved the structure of the colon damaged.
33884622	6	61	theme	proinflammatory	926:940	arg1	cytokines					942:950	proinflammatory cytokines	926:950	proinflammatory cytokines	926:950	Moreover, colonic levels of proinflammatory cytokines were significantly lower following β-carotene supplementation.
33884622	0	62	from	colitis	117:123	arg1	rats					128:131	rats	128:131	rats	128:131	Gut microbiota regulation and anti-inflammatory effect of β-carotene in dextran sulfate sodium-stimulated ulcerative colitis in rats.
33884622	11	63	theme	gut	1669:1671	arg1	microbiota					1673:1682	gut microbiota	1669:1682	gut microbiota	1669:1682	β-Carotene can alleviate DSS-induced UC through the regulation of gut microbiota.
33884622	0	64	theme	dextran	72:78	arg1	sulfate					80:86	dextran sulfate	72:86	dextran sulfate	72:86	Gut microbiota regulation and anti-inflammatory effect of β-carotene in dextran sulfate sodium-stimulated ulcerative colitis in rats.
33884622	9	65	theme	abundance	1406:1414	arg1	levels					1441:1446	the levels	1437:1446	the levels	1437:1446	Furthermore, β-carotene treatment significantly increased the abundance of Faecalibacterium, the levels of which negatively correlated with the levels of inflammatory cytokines.
33884622	8	66	from	levels	1328:1333	arg1	structure					1294:1302	the microbial structure	1280:1302	the microbial structure at the phylum and genus levels of rats	1280:1341	The result of the relative abundance of gut microbiota showed that DSS administration significantly changed the microbial structure at the phylum and genus levels of rats.
33884622	9	67	theme	Faecalibacterium	1419:1434	arg1	abundance					1406:1414	the abundance	1402:1414	the abundance	1402:1414	Furthermore, β-carotene treatment significantly increased the abundance of Faecalibacterium, the levels of which negatively correlated with the levels of inflammatory cytokines.
33884622	5	68	dep	significantly	677:689	arg1	p < 0.05					692:699	p < 0.05	692:699	p < 0.05	692:699	The results revealed that β-carotene significantly (p < 0.05) decreased the severity of colitis in rats, as assessed using body weight (6.00 ± 1.73%), colon length (22.23 ± 0.53%), and disease activity index, and improved the structure of the colon damaged.
33884622	8	69	theme	relative	1190:1197	arg1	abundance					1199:1207	the relative abundance	1186:1207	the relative abundance of gut microbiota	1186:1225	The result of the relative abundance of gut microbiota showed that DSS administration significantly changed the microbial structure at the phylum and genus levels of rats.
33884622	0	70	from	effect	48:53	arg1	sulfate					80:86	dextran sulfate	72:86	dextran sulfate	72:86	Gut microbiota regulation and anti-inflammatory effect of β-carotene in dextran sulfate sodium-stimulated ulcerative colitis in rats.
33884622	8	71	theme	microbiota	1216:1225	arg1	abundance					1199:1207	the relative abundance	1186:1207	the relative abundance of gut microbiota	1186:1225	The result of the relative abundance of gut microbiota showed that DSS administration significantly changed the microbial structure at the phylum and genus levels of rats.
33884622	3	72	theme	improvement	433:443	arg1	modulation					397:406	the modulation	393:406	the modulation of gut microbiota and UC improvement	393:443	However, the role of β-carotene in the modulation of gut microbiota and UC improvement is unclear.
33884622	5	73	theme	body	763:766	arg1	weight					768:773	body weight	763:773	body weight (6.00 ± 1.73%)	763:788	The results revealed that β-carotene significantly (p < 0.05) decreased the severity of colitis in rats, as assessed using body weight (6.00 ± 1.73%), colon length (22.23 ± 0.53%), and disease activity index, and improved the structure of the colon damaged.
33884622	5	73	theme	body	763:766	arg1	%					787:787	6.00 ± 1.73%	776:787	6.00 ± 1.73%	776:787	The results revealed that β-carotene significantly (p < 0.05) decreased the severity of colitis in rats, as assessed using body weight (6.00 ± 1.73%), colon length (22.23 ± 0.53%), and disease activity index, and improved the structure of the colon damaged.
33884622	6	74	theme	β-carotene	987:996	arg1	supplementation					998:1012	β-carotene supplementation	987:1012	β-carotene supplementation	987:1012	Moreover, colonic levels of proinflammatory cytokines were significantly lower following β-carotene supplementation.
33884622	4	75	theme	gut	548:550	arg1	microbiota					552:561	gut microbiota	548:561	gut microbiota	548:561	In this research, the properties of β-carotene on anti-inflammatory and the composition of gut microbiota were evaluated in a rat model of UC induced by dextran sulfate sodium (DSS).
33884622	12	76	theme	UC	1772:1773	arg1	treatment					1759:1767	the treatment	1755:1767	the treatment of UC	1755:1773	This study provides a reference for the rational use of β-carotene in the treatment of UC.
33884622	4	77	from	composition	533:543	arg1	anti-inflammatory					507:523	anti-inflammatory	507:523	anti-inflammatory	507:523	In this research, the properties of β-carotene on anti-inflammatory and the composition of gut microbiota were evaluated in a rat model of UC induced by dextran sulfate sodium (DSS).
33884622	3	78	theme	gut	411:413	arg1	microbiota					415:424	gut microbiota	411:424	gut microbiota	411:424	However, the role of β-carotene in the modulation of gut microbiota and UC improvement is unclear.
33884622	13	79	theme	health	1934:1939	arg1	intervention					1890:1901	the nutritional intervention	1874:1901	the nutritional intervention of β-carotene enhancing animal health	1874:1939	PRACTICAL APPLICATION: β-Carotene can relieve ulcerative colitis and regulate the gut microbiota; the nutritional intervention of β-carotene enhancing animal health.
33884622	7	80	theme	phosphorylated	1077:1090	arg1	Erk					1130:1132	Erk	1130:1132	Erk (0.63 ± 0.04)	1130:1146	β-Carotene intervention also lowered the expression levels of phosphorylated p65 (0.60 ± 0.02), p38 (0.57 ± 0.00), Erk (0.63 ± 0.04), and JNK (0.70 ± 0.00).
33884622	7	80	theme	phosphorylated	1077:1090	arg1	0.60 ±					1097:1102	0.60 ± 0.02	1097:1107	0.60 ± 0.02	1097:1107	β-Carotene intervention also lowered the expression levels of phosphorylated p65 (0.60 ± 0.02), p38 (0.57 ± 0.00), Erk (0.63 ± 0.04), and JNK (0.70 ± 0.00).
33884622	7	80	theme	phosphorylated	1077:1090	arg1	p65					1092:1094	phosphorylated p65	1077:1094	phosphorylated p65 (0.60 ± 0.02)	1077:1108	β-Carotene intervention also lowered the expression levels of phosphorylated p65 (0.60 ± 0.02), p38 (0.57 ± 0.00), Erk (0.63 ± 0.04), and JNK (0.70 ± 0.00).
33884622	7	80	theme	phosphorylated	1077:1090	arg1	JNK					1153:1155	JNK	1153:1155	JNK (0.70 ± 0.00)	1153:1169	β-Carotene intervention also lowered the expression levels of phosphorylated p65 (0.60 ± 0.02), p38 (0.57 ± 0.00), Erk (0.63 ± 0.04), and JNK (0.70 ± 0.00).
33884622	7	80	theme	phosphorylated	1077:1090	arg1	p38					1111:1113	p38	1111:1113	p38 (0.57 ± 0.00)	1111:1127	β-Carotene intervention also lowered the expression levels of phosphorylated p65 (0.60 ± 0.02), p38 (0.57 ± 0.00), Erk (0.63 ± 0.04), and JNK (0.70 ± 0.00).
33884622	9	81	theme	inflammatory	1498:1509	arg1	cytokines					1511:1519	inflammatory cytokines	1498:1519	inflammatory cytokines	1498:1519	Furthermore, β-carotene treatment significantly increased the abundance of Faecalibacterium, the levels of which negatively correlated with the levels of inflammatory cytokines.
33884622	8	82	theme	microbial	1284:1292	arg1	structure					1294:1302	the microbial structure	1280:1302	the microbial structure at the phylum and genus levels of rats	1280:1341	The result of the relative abundance of gut microbiota showed that DSS administration significantly changed the microbial structure at the phylum and genus levels of rats.
33884622	2	83	theme	colon	344:348	arg1	cancer					350:355	colon cancer	344:355	colon cancer	344:355	Ulcerative colitis (UC) is a kind of inflammatory bowel disease that is accompanied by a certain risk of colon cancer.
33884622	10	84	from	target	1558:1563	arg1	alleviation					1572:1582	the alleviation	1568:1582	the alleviation of DSS-induced UC	1568:1600	Faecalibacterium may be a potential target in the alleviation of DSS-induced UC.
33884622	10	85	theme	UC	1599:1600	arg1	alleviation					1572:1582	the alleviation	1568:1582	the alleviation of DSS-induced UC	1568:1600	Faecalibacterium may be a potential target in the alleviation of DSS-induced UC.
33884622	8	86	theme	phylum	1311:1316	arg1	levels					1328:1333	the phylum and genus levels	1307:1333	the phylum and genus levels of rats	1307:1341	The result of the relative abundance of gut microbiota showed that DSS administration significantly changed the microbial structure at the phylum and genus levels of rats.
33884622	13	87	theme	enhancing	1917:1925	arg1	health					1934:1939	β-carotene enhancing animal health	1906:1939	β-carotene enhancing animal health	1906:1939	PRACTICAL APPLICATION: β-Carotene can relieve ulcerative colitis and regulate the gut microbiota; the nutritional intervention of β-carotene enhancing animal health.
33884622	0	88	theme	microbiota	4:13	arg1	regulation					15:24	Gut microbiota regulation	0:24	Gut microbiota regulation	0:24	Gut microbiota regulation and anti-inflammatory effect of β-carotene in dextran sulfate sodium-stimulated ulcerative colitis in rats.
33794252	0	0	theme	BALB-c	78:83	arg1	mice					85:88	BALB-c mice	78:88	BALB-c mice	78:88	Co-administration of 5FU and propolis on AOM/DSS induced colorectal cancer in BALB-c mice.
33794252	5	1	theme	CRC	699:701	arg1	sulfate					740:746	treated and untreated CRC model [azoxymethane + dextran sodium sulfate]	677:747	treated and untreated CRC model [azoxymethane + dextran sodium sulfate]	677:747	In this study, 49 male Balb/c mice (16-20 g) were divided in seven groups as a control group and experimental groups (treated and untreated CRC model [azoxymethane + dextran sodium sulfate]).
33794252	5	1	theme	CRC	699:701	arg1	groups					669:674	experimental groups	656:674	experimental groups (treated and untreated CRC model [azoxymethane + dextran sodium sulfate])	656:748	In this study, 49 male Balb/c mice (16-20 g) were divided in seven groups as a control group and experimental groups (treated and untreated CRC model [azoxymethane + dextran sodium sulfate]).
33794252	9	2	theme	KEY	1193:1195	arg1	analysis					1212:1219	KEY FINDINGS GC-MS analysis	1193:1219	KEY FINDINGS GC-MS analysis of propolis	1193:1231	KEY FINDINGS GC-MS analysis of propolis showed the presence of hydrocarbons, alcohols, ketones, terpenes, phenols, and flavonoids.
33794252	7	3	theme	propolis	826:833	arg1	effects					815:821	the anti-cancer effects	799:821	the anti-cancer effects of propolis	799:833	To examine the anti-cancer effects of propolis, the number of aberrant crypt foci (ACF) was counted and the pathological lesions in the distal colonic epithelial tissue were diagnosed.
33794252	5	4	theme	model	703:707	arg1	sulfate					740:746	treated and untreated CRC model [azoxymethane + dextran sodium sulfate]	677:747	treated and untreated CRC model [azoxymethane + dextran sodium sulfate]	677:747	In this study, 49 male Balb/c mice (16-20 g) were divided in seven groups as a control group and experimental groups (treated and untreated CRC model [azoxymethane + dextran sodium sulfate]).
33794252	5	4	theme	model	703:707	arg1	groups					669:674	experimental groups	656:674	experimental groups (treated and untreated CRC model [azoxymethane + dextran sodium sulfate])	656:748	In this study, 49 male Balb/c mice (16-20 g) were divided in seven groups as a control group and experimental groups (treated and untreated CRC model [azoxymethane + dextran sodium sulfate]).
33794252	3	5	from	cancer	385:390	arg1	models					407:412	mouse models	401:412	mouse models	401:412	This study aims to examine the combined effects of 5-fluorouracil (5FU) and propolis on colorectal cancer (CRC) in mouse models.
33794252	7	6	theme	anti-cancer	803:813	arg1	effects					815:821	the anti-cancer effects	799:821	the anti-cancer effects of propolis	799:833	To examine the anti-cancer effects of propolis, the number of aberrant crypt foci (ACF) was counted and the pathological lesions in the distal colonic epithelial tissue were diagnosed.
33794252	4	7	dep	MATERIALS	415:423	arg1	composition					450:460	The chemical composition	437:460	The chemical composition	437:460	MATERIALS AND METHODS The chemical composition of ethanolic extract of propolis was determined by gas chromatography-mass spectrometry (GC-MS).
33794252	3	8	theme	5-fluorouracil	337:350	arg1	effects					326:332	the combined effects	313:332	the combined effects of 5-fluorouracil (5FU) and propolis on colorectal cancer (CRC) in mouse models	313:412	This study aims to examine the combined effects of 5-fluorouracil (5FU) and propolis on colorectal cancer (CRC) in mouse models.
33794252	0	9	from	Co-administration	0:16	arg1	AOM/DSS					41:47	AOM/DSS	41:47	AOM/DSS	41:47	Co-administration of 5FU and propolis on AOM/DSS induced colorectal cancer in BALB-c mice.
33794252	8	10	dep	synthase	1053:1060	arg1	proteins					1098:1105	proteins	1098:1105	proteins	1098:1105	In this study, the expression of beta-catenin (β-catenin), induced nitric oxide synthase (iNOS) and cyclooxygenase-2 (Cox-2) proteins, which play a major role in the incidence and progression of cancer, were determined.
33794252	2	11	theme	anti-cancer	262:272	arg1	properties					274:283	anti-inflammatory and anti-cancer properties	240:283	anti-inflammatory and anti-cancer properties	240:283	Propolis has biological activities, such as anti-inflammatory and anti-cancer properties.
33794252	9	12	theme	terpenes	1289:1296	arg1	presence					1244:1251	the presence	1240:1251	the presence of hydrocarbons, alcohols, ketones, terpenes, phenols, and flavonoids	1240:1321	KEY FINDINGS GC-MS analysis of propolis showed the presence of hydrocarbons, alcohols, ketones, terpenes, phenols, and flavonoids.
33794252	8	13	dep	incidence	1139:1147	arg1	the					1135:1137	the	1135:1137	the	1135:1137	In this study, the expression of beta-catenin (β-catenin), induced nitric oxide synthase (iNOS) and cyclooxygenase-2 (Cox-2) proteins, which play a major role in the incidence and progression of cancer, were determined.
33794252	5	14	theme	male	577:580	arg1	mice					589:592	49 male Balb/c mice	574:592	49 male Balb/c mice (16-20 g)	574:602	In this study, 49 male Balb/c mice (16-20 g) were divided in seven groups as a control group and experimental groups (treated and untreated CRC model [azoxymethane + dextran sodium sulfate]).
33794252	5	14	theme	male	577:580	arg1	16-20 g					595:601	16-20 g	595:601	16-20 g	595:601	In this study, 49 male Balb/c mice (16-20 g) were divided in seven groups as a control group and experimental groups (treated and untreated CRC model [azoxymethane + dextran sodium sulfate]).
33794252	12	15	theme	adjunct	1724:1730	arg1	propolis					1644:1651	propolis	1644:1651	propolis	1644:1651	SIGNIFICANCE The results showed that propolis increased the efficiency of 5FU and could be taken into account as the adjunct therapy for colorectal cancer.
33794252	12	15	theme	adjunct	1724:1730	arg1	therapy					1732:1738	the adjunct therapy	1720:1738	the adjunct therapy for colorectal cancer	1720:1760	SIGNIFICANCE The results showed that propolis increased the efficiency of 5FU and could be taken into account as the adjunct therapy for colorectal cancer.
33794252	1	16	theme	side	151:154	arg1	problems					116:123	the main problems	107:123	the main problems with chemotherapy	107:141	AIMS Currently, the main problems with chemotherapy are its side effects, toxicity, and drug resistance.
33794252	1	16	theme	side	151:154	arg1	effects					156:162	its side effects	147:162	its side effects	147:162	AIMS Currently, the main problems with chemotherapy are its side effects, toxicity, and drug resistance.
33794252	5	17	theme	treated	677:683	arg1	sulfate					740:746	treated and untreated CRC model [azoxymethane + dextran sodium sulfate]	677:747	treated and untreated CRC model [azoxymethane + dextran sodium sulfate]	677:747	In this study, 49 male Balb/c mice (16-20 g) were divided in seven groups as a control group and experimental groups (treated and untreated CRC model [azoxymethane + dextran sodium sulfate]).
33794252	5	17	theme	treated	677:683	arg1	groups					669:674	experimental groups	656:674	experimental groups (treated and untreated CRC model [azoxymethane + dextran sodium sulfate])	656:748	In this study, 49 male Balb/c mice (16-20 g) were divided in seven groups as a control group and experimental groups (treated and untreated CRC model [azoxymethane + dextran sodium sulfate]).
33794252	9	18	theme	ketones	1280:1286	arg1	presence					1244:1251	the presence	1240:1251	the presence of hydrocarbons, alcohols, ketones, terpenes, phenols, and flavonoids	1240:1321	KEY FINDINGS GC-MS analysis of propolis showed the presence of hydrocarbons, alcohols, ketones, terpenes, phenols, and flavonoids.
33794252	1	19	with	problems	116:123	arg1	chemotherapy					130:141	chemotherapy	130:141	chemotherapy	130:141	AIMS Currently, the main problems with chemotherapy are its side effects, toxicity, and drug resistance.
33794252	7	20	theme	foci	865:868	arg1	number					840:845	the number	836:845	the number of aberrant crypt foci (ACF)	836:874	To examine the anti-cancer effects of propolis, the number of aberrant crypt foci (ACF) was counted and the pathological lesions in the distal colonic epithelial tissue were diagnosed.
33794252	2	21	theme	anti-inflammatory	240:256	arg1	properties					274:283	anti-inflammatory and anti-cancer properties	240:283	anti-inflammatory and anti-cancer properties	240:283	Propolis has biological activities, such as anti-inflammatory and anti-cancer properties.
33794252	3	22	theme	mouse	401:405	arg1	models					407:412	mouse models	401:412	mouse models	401:412	This study aims to examine the combined effects of 5-fluorouracil (5FU) and propolis on colorectal cancer (CRC) in mouse models.
33794252	4	23	theme	chemical	441:448	arg1	composition					450:460	The chemical composition	437:460	The chemical composition	437:460	MATERIALS AND METHODS The chemical composition of ethanolic extract of propolis was determined by gas chromatography-mass spectrometry (GC-MS).
33794252	12	24	theme	colorectal	1744:1753	arg1	cancer					1755:1760	colorectal cancer	1744:1760	colorectal cancer	1744:1760	SIGNIFICANCE The results showed that propolis increased the efficiency of 5FU and could be taken into account as the adjunct therapy for colorectal cancer.
33794252	9	25	theme	FINDINGS	1197:1204	arg1	analysis					1212:1219	KEY FINDINGS GC-MS analysis	1193:1219	KEY FINDINGS GC-MS analysis of propolis	1193:1231	KEY FINDINGS GC-MS analysis of propolis showed the presence of hydrocarbons, alcohols, ketones, terpenes, phenols, and flavonoids.
33794252	3	26	theme	propolis	362:369	arg1	effects					326:332	the combined effects	313:332	the combined effects of 5-fluorouracil (5FU) and propolis on colorectal cancer (CRC) in mouse models	313:412	This study aims to examine the combined effects of 5-fluorouracil (5FU) and propolis on colorectal cancer (CRC) in mouse models.
33794252	4	27	theme	chromatography-mass	517:535	arg1	GC-MS					551:555	GC-MS	551:555	GC-MS	551:555	MATERIALS AND METHODS The chemical composition of ethanolic extract of propolis was determined by gas chromatography-mass spectrometry (GC-MS).
33794252	4	27	theme	chromatography-mass	517:535	arg1	spectrometry					537:548	gas chromatography-mass spectrometry	513:548	gas chromatography-mass spectrometry (GC-MS)	513:556	MATERIALS AND METHODS The chemical composition of ethanolic extract of propolis was determined by gas chromatography-mass spectrometry (GC-MS).
33794252	11	28	theme	iNOS	1577:1580	arg1	expression					1556:1565	the expression	1552:1565	the expression of Cox-2, iNOS, and β-catenin proteins	1552:1604	The propolis combined with 5FU reduced the expression of Cox-2, iNOS, and β-catenin proteins.
33794252	7	29	theme	aberrant	850:857	arg1	foci					865:868	aberrant crypt foci	850:868	aberrant crypt foci (ACF)	850:874	To examine the anti-cancer effects of propolis, the number of aberrant crypt foci (ACF) was counted and the pathological lesions in the distal colonic epithelial tissue were diagnosed.
33794252	7	29	theme	aberrant	850:857	arg1	ACF					871:873	ACF	871:873	ACF	871:873	To examine the anti-cancer effects of propolis, the number of aberrant crypt foci (ACF) was counted and the pathological lesions in the distal colonic epithelial tissue were diagnosed.
33794252	5	30	theme	experimental	656:667	arg1	sulfate					740:746	treated and untreated CRC model [azoxymethane + dextran sodium sulfate]	677:747	treated and untreated CRC model [azoxymethane + dextran sodium sulfate]	677:747	In this study, 49 male Balb/c mice (16-20 g) were divided in seven groups as a control group and experimental groups (treated and untreated CRC model [azoxymethane + dextran sodium sulfate]).
33794252	5	30	theme	experimental	656:667	arg1	groups					669:674	experimental groups	656:674	experimental groups (treated and untreated CRC model [azoxymethane + dextran sodium sulfate])	656:748	In this study, 49 male Balb/c mice (16-20 g) were divided in seven groups as a control group and experimental groups (treated and untreated CRC model [azoxymethane + dextran sodium sulfate]).
33794252	7	31	from	lesions	909:915	arg1	tissue					950:955	the distal colonic epithelial tissue	920:955	the distal colonic epithelial tissue	920:955	To examine the anti-cancer effects of propolis, the number of aberrant crypt foci (ACF) was counted and the pathological lesions in the distal colonic epithelial tissue were diagnosed.
33794252	4	32	theme	gas	513:515	arg1	GC-MS					551:555	GC-MS	551:555	GC-MS	551:555	MATERIALS AND METHODS The chemical composition of ethanolic extract of propolis was determined by gas chromatography-mass spectrometry (GC-MS).
33794252	4	32	theme	gas	513:515	arg1	spectrometry					537:548	gas chromatography-mass spectrometry	513:548	gas chromatography-mass spectrometry (GC-MS)	513:556	MATERIALS AND METHODS The chemical composition of ethanolic extract of propolis was determined by gas chromatography-mass spectrometry (GC-MS).
33794252	7	33	theme	crypt	859:863	arg1	foci					865:868	aberrant crypt foci	850:868	aberrant crypt foci (ACF)	850:874	To examine the anti-cancer effects of propolis, the number of aberrant crypt foci (ACF) was counted and the pathological lesions in the distal colonic epithelial tissue were diagnosed.
33794252	7	33	theme	crypt	859:863	arg1	ACF					871:873	ACF	871:873	ACF	871:873	To examine the anti-cancer effects of propolis, the number of aberrant crypt foci (ACF) was counted and the pathological lesions in the distal colonic epithelial tissue were diagnosed.
33794252	9	34	theme	flavonoids	1312:1321	arg1	presence					1244:1251	the presence	1240:1251	the presence of hydrocarbons, alcohols, ketones, terpenes, phenols, and flavonoids	1240:1321	KEY FINDINGS GC-MS analysis of propolis showed the presence of hydrocarbons, alcohols, ketones, terpenes, phenols, and flavonoids.
33794252	0	35	theme	5FU	21:23	arg1	Co-administration					0:16	Co-administration	0:16	Co-administration of 5FU and propolis on AOM/DSS	0:47	Co-administration of 5FU and propolis on AOM/DSS induced colorectal cancer in BALB-c mice.
33794252	11	36	theme	proteins	1597:1604	arg1	expression					1556:1565	the expression	1552:1565	the expression of Cox-2, iNOS, and β-catenin proteins	1552:1604	The propolis combined with 5FU reduced the expression of Cox-2, iNOS, and β-catenin proteins.
33794252	5	37	theme	control	638:644	arg1	group					646:650	a control group	636:650	a control group	636:650	In this study, 49 male Balb/c mice (16-20 g) were divided in seven groups as a control group and experimental groups (treated and untreated CRC model [azoxymethane + dextran sodium sulfate]).
33794252	3	38	from	effects	326:332	arg1	CRC					393:395	CRC	393:395	CRC	393:395	This study aims to examine the combined effects of 5-fluorouracil (5FU) and propolis on colorectal cancer (CRC) in mouse models.
33794252	3	38	from	effects	326:332	arg1	cancer					385:390	colorectal cancer	374:390	colorectal cancer (CRC) in mouse models	374:412	This study aims to examine the combined effects of 5-fluorouracil (5FU) and propolis on colorectal cancer (CRC) in mouse models.
33794252	10	39	theme	pathological	1402:1413	arg1	lesions					1415:1421	pathological lesions	1402:1421	pathological lesions	1402:1421	Administering propolis in combination with 5FU reduced the number of ACFs and pathological lesions in comparison with cancer control groups (p < 0.0001) and 5FU-alone treatment (p < 0.05).
33794252	5	40	theme	[azoxymethane + dextran	709:731	arg1	sulfate					740:746	treated and untreated CRC model [azoxymethane + dextran sodium sulfate]	677:747	treated and untreated CRC model [azoxymethane + dextran sodium sulfate]	677:747	In this study, 49 male Balb/c mice (16-20 g) were divided in seven groups as a control group and experimental groups (treated and untreated CRC model [azoxymethane + dextran sodium sulfate]).
33794252	5	40	theme	[azoxymethane + dextran	709:731	arg1	groups					669:674	experimental groups	656:674	experimental groups (treated and untreated CRC model [azoxymethane + dextran sodium sulfate])	656:748	In this study, 49 male Balb/c mice (16-20 g) were divided in seven groups as a control group and experimental groups (treated and untreated CRC model [azoxymethane + dextran sodium sulfate]).
33794252	10	41	dep	cancer	1442:1447	arg1	p < 0.0001					1465:1474	p < 0.0001	1465:1474	p < 0.0001	1465:1474	Administering propolis in combination with 5FU reduced the number of ACFs and pathological lesions in comparison with cancer control groups (p < 0.0001) and 5FU-alone treatment (p < 0.05).
33794252	10	41	dep	cancer	1442:1447	arg1	groups					1457:1462	control groups	1449:1462	control groups (p < 0.0001)	1449:1475	Administering propolis in combination with 5FU reduced the number of ACFs and pathological lesions in comparison with cancer control groups (p < 0.0001) and 5FU-alone treatment (p < 0.05).
33794252	12	42	dep	SIGNIFICANCE	1607:1618	arg1	results					1624:1630	The results	1620:1630	SIGNIFICANCE The results	1607:1630	SIGNIFICANCE The results showed that propolis increased the efficiency of 5FU and could be taken into account as the adjunct therapy for colorectal cancer.
33794252	2	43	theme	biological	209:218	arg1	properties					274:283	anti-inflammatory and anti-cancer properties	240:283	anti-inflammatory and anti-cancer properties	240:283	Propolis has biological activities, such as anti-inflammatory and anti-cancer properties.
33794252	2	43	theme	biological	209:218	arg1	activities					220:229	biological activities	209:229	biological activities	209:229	Propolis has biological activities, such as anti-inflammatory and anti-cancer properties.
33794252	8	44	theme	major	1121:1125	arg1	role					1127:1130	a major role	1119:1130	a major role	1119:1130	In this study, the expression of beta-catenin (β-catenin), induced nitric oxide synthase (iNOS) and cyclooxygenase-2 (Cox-2) proteins, which play a major role in the incidence and progression of cancer, were determined.
33794252	1	45	theme	drug	179:182	arg1	resistance					184:193	drug resistance	179:193	drug resistance	179:193	AIMS Currently, the main problems with chemotherapy are its side effects, toxicity, and drug resistance.
33794252	0	46	theme	propolis	29:36	arg1	Co-administration					0:16	Co-administration	0:16	Co-administration of 5FU and propolis on AOM/DSS	0:47	Co-administration of 5FU and propolis on AOM/DSS induced colorectal cancer in BALB-c mice.
33794252	9	47	theme	propolis	1224:1231	arg1	analysis					1212:1219	KEY FINDINGS GC-MS analysis	1193:1219	KEY FINDINGS GC-MS analysis of propolis	1193:1231	KEY FINDINGS GC-MS analysis of propolis showed the presence of hydrocarbons, alcohols, ketones, terpenes, phenols, and flavonoids.
33794252	5	48	theme	sodium	733:738	arg1	sulfate					740:746	treated and untreated CRC model [azoxymethane + dextran sodium sulfate]	677:747	treated and untreated CRC model [azoxymethane + dextran sodium sulfate]	677:747	In this study, 49 male Balb/c mice (16-20 g) were divided in seven groups as a control group and experimental groups (treated and untreated CRC model [azoxymethane + dextran sodium sulfate]).
33794252	5	48	theme	sodium	733:738	arg1	groups					669:674	experimental groups	656:674	experimental groups (treated and untreated CRC model [azoxymethane + dextran sodium sulfate])	656:748	In this study, 49 male Balb/c mice (16-20 g) were divided in seven groups as a control group and experimental groups (treated and untreated CRC model [azoxymethane + dextran sodium sulfate]).
33794252	8	49	theme	nitric	1040:1045	arg1	iNOS					1063:1066	iNOS	1063:1066	iNOS	1063:1066	In this study, the expression of beta-catenin (β-catenin), induced nitric oxide synthase (iNOS) and cyclooxygenase-2 (Cox-2) proteins, which play a major role in the incidence and progression of cancer, were determined.
33794252	8	49	theme	nitric	1040:1045	arg1	synthase					1053:1060	nitric oxide synthase	1040:1060	nitric oxide synthase (iNOS)	1040:1067	In this study, the expression of beta-catenin (β-catenin), induced nitric oxide synthase (iNOS) and cyclooxygenase-2 (Cox-2) proteins, which play a major role in the incidence and progression of cancer, were determined.
33794252	9	50	theme	GC-MS	1206:1210	arg1	analysis					1212:1219	KEY FINDINGS GC-MS analysis	1193:1219	KEY FINDINGS GC-MS analysis of propolis	1193:1231	KEY FINDINGS GC-MS analysis of propolis showed the presence of hydrocarbons, alcohols, ketones, terpenes, phenols, and flavonoids.
33794252	7	51	theme	distal	924:929	arg1	tissue					950:955	the distal colonic epithelial tissue	920:955	the distal colonic epithelial tissue	920:955	To examine the anti-cancer effects of propolis, the number of aberrant crypt foci (ACF) was counted and the pathological lesions in the distal colonic epithelial tissue were diagnosed.
33794252	4	52	theme	propolis	486:493	arg1	extract					475:481	ethanolic extract	465:481	ethanolic extract of propolis	465:493	MATERIALS AND METHODS The chemical composition of ethanolic extract of propolis was determined by gas chromatography-mass spectrometry (GC-MS).
33794252	0	53	theme	colorectal	57:66	arg1	cancer					68:73	colorectal cancer	57:73	colorectal cancer	57:73	Co-administration of 5FU and propolis on AOM/DSS induced colorectal cancer in BALB-c mice.
33794252	7	54	theme	colonic	931:937	arg1	tissue					950:955	the distal colonic epithelial tissue	920:955	the distal colonic epithelial tissue	920:955	To examine the anti-cancer effects of propolis, the number of aberrant crypt foci (ACF) was counted and the pathological lesions in the distal colonic epithelial tissue were diagnosed.
33794252	3	55	theme	colorectal	374:383	arg1	CRC					393:395	CRC	393:395	CRC	393:395	This study aims to examine the combined effects of 5-fluorouracil (5FU) and propolis on colorectal cancer (CRC) in mouse models.
33794252	3	55	theme	colorectal	374:383	arg1	cancer					385:390	colorectal cancer	374:390	colorectal cancer (CRC) in mouse models	374:412	This study aims to examine the combined effects of 5-fluorouracil (5FU) and propolis on colorectal cancer (CRC) in mouse models.
33794252	8	56	theme	beta-catenin	1006:1017	arg1	expression					992:1001	the expression	988:1001	the expression	988:1001	In this study, the expression of beta-catenin (β-catenin), induced nitric oxide synthase (iNOS) and cyclooxygenase-2 (Cox-2) proteins, which play a major role in the incidence and progression of cancer, were determined.
33794252	5	57	theme	Balb/c	582:587	arg1	mice					589:592	49 male Balb/c mice	574:592	49 male Balb/c mice (16-20 g)	574:602	In this study, 49 male Balb/c mice (16-20 g) were divided in seven groups as a control group and experimental groups (treated and untreated CRC model [azoxymethane + dextran sodium sulfate]).
33794252	5	57	theme	Balb/c	582:587	arg1	16-20 g					595:601	16-20 g	595:601	16-20 g	595:601	In this study, 49 male Balb/c mice (16-20 g) were divided in seven groups as a control group and experimental groups (treated and untreated CRC model [azoxymethane + dextran sodium sulfate]).
33794252	12	58	theme	5FU	1681:1683	arg1	efficiency					1667:1676	the efficiency	1663:1676	the efficiency of 5FU	1663:1683	SIGNIFICANCE The results showed that propolis increased the efficiency of 5FU and could be taken into account as the adjunct therapy for colorectal cancer.
33794252	10	59	with	combination	1350:1360	arg1	5FU					1367:1369	5FU	1367:1369	5FU	1367:1369	Administering propolis in combination with 5FU reduced the number of ACFs and pathological lesions in comparison with cancer control groups (p < 0.0001) and 5FU-alone treatment (p < 0.05).
33794252	7	60	theme	epithelial	939:948	arg1	tissue					950:955	the distal colonic epithelial tissue	920:955	the distal colonic epithelial tissue	920:955	To examine the anti-cancer effects of propolis, the number of aberrant crypt foci (ACF) was counted and the pathological lesions in the distal colonic epithelial tissue were diagnosed.
33794252	10	61	with	comparison	1426:1435	arg1	treatment					1491:1499	5FU-alone treatment	1481:1499	5FU-alone treatment (p < 0.05)	1481:1510	Administering propolis in combination with 5FU reduced the number of ACFs and pathological lesions in comparison with cancer control groups (p < 0.0001) and 5FU-alone treatment (p < 0.05).
33794252	10	61	with	comparison	1426:1435	arg1	p < 0.05					1502:1509	p < 0.05	1502:1509	p < 0.05	1502:1509	Administering propolis in combination with 5FU reduced the number of ACFs and pathological lesions in comparison with cancer control groups (p < 0.0001) and 5FU-alone treatment (p < 0.05).
33794252	10	61	with	comparison	1426:1435	arg1	cancer					1442:1447	cancer control groups (p < 0.0001) and 5FU-alone treatment (p < 0.05)	1442:1510	cancer	1442:1447	Administering propolis in combination with 5FU reduced the number of ACFs and pathological lesions in comparison with cancer control groups (p < 0.0001) and 5FU-alone treatment (p < 0.05).
33794252	5	62	theme	untreated	689:697	arg1	sulfate					740:746	treated and untreated CRC model [azoxymethane + dextran sodium sulfate]	677:747	treated and untreated CRC model [azoxymethane + dextran sodium sulfate]	677:747	In this study, 49 male Balb/c mice (16-20 g) were divided in seven groups as a control group and experimental groups (treated and untreated CRC model [azoxymethane + dextran sodium sulfate]).
33794252	5	62	theme	untreated	689:697	arg1	groups					669:674	experimental groups	656:674	experimental groups (treated and untreated CRC model [azoxymethane + dextran sodium sulfate])	656:748	In this study, 49 male Balb/c mice (16-20 g) were divided in seven groups as a control group and experimental groups (treated and untreated CRC model [azoxymethane + dextran sodium sulfate]).
33794252	1	63	theme	main	111:114	arg1	problems					116:123	the main problems	107:123	the main problems with chemotherapy	107:141	AIMS Currently, the main problems with chemotherapy are its side effects, toxicity, and drug resistance.
33794252	1	63	theme	main	111:114	arg1	effects					156:162	its side effects	147:162	its side effects	147:162	AIMS Currently, the main problems with chemotherapy are its side effects, toxicity, and drug resistance.
33794252	11	64	theme	β-catenin	1587:1595	arg1	proteins					1597:1604	β-catenin proteins	1587:1604	β-catenin proteins	1587:1604	The propolis combined with 5FU reduced the expression of Cox-2, iNOS, and β-catenin proteins.
33794252	4	65	theme	extract	475:481	arg1	METHODS					429:435	METHODS	429:435	METHODS	429:435	MATERIALS AND METHODS The chemical composition of ethanolic extract of propolis was determined by gas chromatography-mass spectrometry (GC-MS).
33794252	4	65	theme	extract	475:481	arg1	MATERIALS					415:423	MATERIALS	415:423	MATERIALS	415:423	MATERIALS AND METHODS The chemical composition of ethanolic extract of propolis was determined by gas chromatography-mass spectrometry (GC-MS).
33794252	7	66	theme	pathological	896:907	arg1	lesions					909:915	the pathological lesions	892:915	the pathological lesions in the distal colonic epithelial tissue	892:955	To examine the anti-cancer effects of propolis, the number of aberrant crypt foci (ACF) was counted and the pathological lesions in the distal colonic epithelial tissue were diagnosed.
33794252	8	67	theme	cancer	1168:1173	arg1	progression					1153:1163	progression	1153:1163	progression	1153:1163	In this study, the expression of beta-catenin (β-catenin), induced nitric oxide synthase (iNOS) and cyclooxygenase-2 (Cox-2) proteins, which play a major role in the incidence and progression of cancer, were determined.
33794252	8	67	theme	cancer	1168:1173	arg1	incidence					1139:1147	incidence	1139:1147	incidence	1139:1147	In this study, the expression of beta-catenin (β-catenin), induced nitric oxide synthase (iNOS) and cyclooxygenase-2 (Cox-2) proteins, which play a major role in the incidence and progression of cancer, were determined.
33794252	8	68	theme	oxide	1047:1051	arg1	iNOS					1063:1066	iNOS	1063:1066	iNOS	1063:1066	In this study, the expression of beta-catenin (β-catenin), induced nitric oxide synthase (iNOS) and cyclooxygenase-2 (Cox-2) proteins, which play a major role in the incidence and progression of cancer, were determined.
33794252	8	68	theme	oxide	1047:1051	arg1	synthase					1053:1060	nitric oxide synthase	1040:1060	nitric oxide synthase (iNOS)	1040:1067	In this study, the expression of beta-catenin (β-catenin), induced nitric oxide synthase (iNOS) and cyclooxygenase-2 (Cox-2) proteins, which play a major role in the incidence and progression of cancer, were determined.
33794252	9	69	theme	hydrocarbons	1256:1267	arg1	presence					1244:1251	the presence	1240:1251	the presence of hydrocarbons, alcohols, ketones, terpenes, phenols, and flavonoids	1240:1321	KEY FINDINGS GC-MS analysis of propolis showed the presence of hydrocarbons, alcohols, ketones, terpenes, phenols, and flavonoids.
33794252	10	70	theme	lesions	1415:1421	arg1	number					1383:1388	the number	1379:1388	the number of ACFs and pathological lesions	1379:1421	Administering propolis in combination with 5FU reduced the number of ACFs and pathological lesions in comparison with cancer control groups (p < 0.0001) and 5FU-alone treatment (p < 0.05).
33794252	11	71	theme	Cox-2	1570:1574	arg1	expression					1556:1565	the expression	1552:1565	the expression of Cox-2, iNOS, and β-catenin proteins	1552:1604	The propolis combined with 5FU reduced the expression of Cox-2, iNOS, and β-catenin proteins.
33794252	10	72	theme	ACFs	1393:1396	arg1	number					1383:1388	the number	1379:1388	the number of ACFs and pathological lesions	1379:1421	Administering propolis in combination with 5FU reduced the number of ACFs and pathological lesions in comparison with cancer control groups (p < 0.0001) and 5FU-alone treatment (p < 0.05).
33794252	9	73	theme	phenols	1299:1305	arg1	presence					1244:1251	the presence	1240:1251	the presence of hydrocarbons, alcohols, ketones, terpenes, phenols, and flavonoids	1240:1321	KEY FINDINGS GC-MS analysis of propolis showed the presence of hydrocarbons, alcohols, ketones, terpenes, phenols, and flavonoids.
33794252	2	74	contain	has	205:207	arg2	properties					274:283	anti-inflammatory and anti-cancer properties	240:283	anti-inflammatory and anti-cancer properties	240:283	Propolis has biological activities, such as anti-inflammatory and anti-cancer properties.
33794252	2	74	contain	has	205:207	arg1	Propolis					196:203	Propolis	196:203	Propolis	196:203	Propolis has biological activities, such as anti-inflammatory and anti-cancer properties.
33794252	2	74	contain	has	205:207	arg2	activities					220:229	biological activities	209:229	biological activities	209:229	Propolis has biological activities, such as anti-inflammatory and anti-cancer properties.
33794252	3	75	theme	combined	317:324	arg1	effects					326:332	the combined effects	313:332	the combined effects of 5-fluorouracil (5FU) and propolis on colorectal cancer (CRC) in mouse models	313:412	This study aims to examine the combined effects of 5-fluorouracil (5FU) and propolis on colorectal cancer (CRC) in mouse models.
33794252	4	76	theme	ethanolic	465:473	arg1	extract					475:481	ethanolic extract	465:481	ethanolic extract of propolis	465:493	MATERIALS AND METHODS The chemical composition of ethanolic extract of propolis was determined by gas chromatography-mass spectrometry (GC-MS).
33794252	10	77	theme	control	1449:1455	arg1	p < 0.0001					1465:1474	p < 0.0001	1465:1474	p < 0.0001	1465:1474	Administering propolis in combination with 5FU reduced the number of ACFs and pathological lesions in comparison with cancer control groups (p < 0.0001) and 5FU-alone treatment (p < 0.05).
33794252	10	77	theme	control	1449:1455	arg1	groups					1457:1462	control groups	1449:1462	control groups (p < 0.0001)	1449:1475	Administering propolis in combination with 5FU reduced the number of ACFs and pathological lesions in comparison with cancer control groups (p < 0.0001) and 5FU-alone treatment (p < 0.05).
33794252	10	78	theme	5FU-alone	1481:1489	arg1	p < 0.05					1502:1509	p < 0.05	1502:1509	p < 0.05	1502:1509	Administering propolis in combination with 5FU reduced the number of ACFs and pathological lesions in comparison with cancer control groups (p < 0.0001) and 5FU-alone treatment (p < 0.05).
33794252	10	78	theme	5FU-alone	1481:1489	arg1	treatment					1491:1499	5FU-alone treatment	1481:1499	5FU-alone treatment (p < 0.05)	1481:1510	Administering propolis in combination with 5FU reduced the number of ACFs and pathological lesions in comparison with cancer control groups (p < 0.0001) and 5FU-alone treatment (p < 0.05).
33794252	9	79	theme	alcohols	1270:1277	arg1	presence					1244:1251	the presence	1240:1251	the presence of hydrocarbons, alcohols, ketones, terpenes, phenols, and flavonoids	1240:1321	KEY FINDINGS GC-MS analysis of propolis showed the presence of hydrocarbons, alcohols, ketones, terpenes, phenols, and flavonoids.
33298571	13	0	from	glycogen	1672:1679	arg1	vagina					1716:1721	the vagina	1712:1721	the vagina	1712:1721	This work also suggests that the link between glycogen, amylase, and Lactobacillus in the vagina is complex.IMPORTANCE In this study, we show that multiple bacteria in the vaginal community produce amylases that hydrolyze glycogen into simpler sugars (i.e., maltose and maltotriose).
33298571	9	1	theme	vaginal	1140:1146	arg1	fluids					1148:1153	vaginal fluids	1140:1153	vaginal fluids	1140:1153	Although we detected bacterial amylases in vaginal fluids, there was no clear association between the bacterial species that was dominant in a community and the level of amylase activity.
33298571	12	2	from	present	1588:1594	arg1	vagina					1618:1623	the vagina	1614:1623	the vagina	1614:1623	Our findings show, for the first time, that multiple amylases from both bacterial and human origins can be present simultaneously in the vagina.
33298571	1	3	from	Dominance	30:38	arg1	communities					76:86	vaginal communities	68:86	vaginal communities	68:86	Dominance of Lactobacillus species in vaginal communities is a hallmark of healthy conditions in the female genital tract.
33298571	6	4	theme	assembled	850:858	arg1	metagenomes					870:880	the assembled bacterial metagenomes	846:880	the assembled bacterial metagenomes	846:880	Of eight putative bacterial amylases identified in the assembled bacterial metagenomes, we detected four in vaginal fluids.
33298571	15	5	theme	vaginal	2064:2070	arg1	microbiome					2072:2081	the human vaginal microbiome	2054:2081	the human vaginal microbiome	2054:2081	Given the temporal changes that are observed in the human vaginal microbiome, we expect the kinds of bacterial amylases produced will also vary over time.
33298571	4	6	theme	lactic	632:637	arg1	acid					639:642	lactic acid	632:642	lactic acid	632:642	We screened cervicovaginal mucus from 23 reproductive-age women, characterized the species composition of vaginal communities, measured vaginal pH, and determined levels of amylase activity, glycogen, and lactic acid.
33298571	9	7	theme	clear	1169:1173	arg1	association					1175:1185	no clear association	1166:1185	no clear association between the bacterial species that was dominant in a community and the level of amylase activity	1166:1282	Although we detected bacterial amylases in vaginal fluids, there was no clear association between the bacterial species that was dominant in a community and the level of amylase activity.
33298571	10	8	theme	α-amylase	1345:1353	arg1	presence					1327:1334	the presence	1323:1334	the presence	1323:1334	This association was likely masked by the presence of human α-amylase, which was also detected in vaginal fluids.
33298571	4	9	theme	amylase	600:606	arg1	activity					608:615	amylase activity	600:615	amylase activity	600:615	We screened cervicovaginal mucus from 23 reproductive-age women, characterized the species composition of vaginal communities, measured vaginal pH, and determined levels of amylase activity, glycogen, and lactic acid.
33298571	13	10	from	Lactobacillus	1695:1707	arg1	vagina					1716:1721	the vagina	1712:1721	the vagina	1712:1721	This work also suggests that the link between glycogen, amylase, and Lactobacillus in the vagina is complex.IMPORTANCE In this study, we show that multiple bacteria in the vaginal community produce amylases that hydrolyze glycogen into simpler sugars (i.e., maltose and maltotriose).
33298571	15	11	theme	bacterial	2107:2115	arg1	amylases					2117:2124	bacterial amylases	2107:2124	bacterial amylases produced	2107:2133	Given the temporal changes that are observed in the human vaginal microbiome, we expect the kinds of bacterial amylases produced will also vary over time.
33298571	10	12	located	detected	1371:1378	arg2	presence					1327:1334	the presence	1323:1334	the presence	1323:1334	This association was likely masked by the presence of human α-amylase, which was also detected in vaginal fluids.
33298571	10	12	located	detected	1371:1378	arg1	fluids					1391:1396	vaginal fluids	1383:1396	vaginal fluids	1383:1396	This association was likely masked by the presence of human α-amylase, which was also detected in vaginal fluids.
33298571	5	13	theme	measured	675:682	arg1	variables					684:692	these measured variables	669:692	these measured variables	669:692	Based on differences in these measured variables, one sample from each of four individual donors was selected for metagenomic and proteomic analyses.
33298571	9	14	theme	bacterial	1199:1207	arg1	species					1209:1215	the bacterial species	1195:1215	the bacterial species that was dominant in a community	1195:1248	Although we detected bacterial amylases in vaginal fluids, there was no clear association between the bacterial species that was dominant in a community and the level of amylase activity.
33298571	9	14	theme	bacterial	1199:1207	arg1	dominant					1226:1233	dominant	1226:1233	dominant	1226:1233	Although we detected bacterial amylases in vaginal fluids, there was no clear association between the bacterial species that was dominant in a community and the level of amylase activity.
33298571	7	15	theme	vaginal	981:987	arg1	communities					989:999	different vaginal communities	971:999	different vaginal communities	971:999	These amylases were produced by various bacteria in different vaginal communities.
33298571	6	16	theme	vaginal	903:909	arg1	fluids					911:916	vaginal fluids	903:916	vaginal fluids	903:916	Of eight putative bacterial amylases identified in the assembled bacterial metagenomes, we detected four in vaginal fluids.
33298571	1	17	theme	healthy	105:111	arg1	conditions					113:122	healthy conditions	105:122	healthy conditions	105:122	Dominance of Lactobacillus species in vaginal communities is a hallmark of healthy conditions in the female genital tract.
33298571	12	18	from	vagina	1618:1623	arg1	present					1588:1594	present	1588:1594	present	1588:1594	Our findings show, for the first time, that multiple amylases from both bacterial and human origins can be present simultaneously in the vagina.
33298571	11	19	theme	activity	1430:1437	arg1	levels					1412:1417	the levels	1408:1417	the levels of amylase activity and glycogen	1408:1450	Finally, the levels of amylase activity and glycogen were only weakly associated.
33298571	7	20	theme	various	951:957	arg1	bacteria					959:966	various bacteria	951:966	various bacteria in different vaginal communities	951:999	These amylases were produced by various bacteria in different vaginal communities.
33298571	8	21	from	terms	1048:1052	arg1	communities					1019:1029	no two communities	1012:1029	no two communities	1012:1029	Moreover, no two communities were the same in terms of which bacteria were producing amylases.
33298571	8	21	from	terms	1048:1052	arg1	same					1040:1043	same	1040:1043	same	1040:1043	Moreover, no two communities were the same in terms of which bacteria were producing amylases.
33298571	16	22	theme	resources	2201:2209	arg1	pool					2193:2196	the pool	2189:2196	the pool of resources that are broadly shared and shape the species composition of the vaginal bacterial community	2189:2302	These differences influence the pool of resources that are broadly shared and shape the species composition of the vaginal bacterial community.
33298571	1	23	theme	female	131:136	arg1	tract					146:150	the female genital tract	127:150	the female genital tract	127:150	Dominance of Lactobacillus species in vaginal communities is a hallmark of healthy conditions in the female genital tract.
33298571	8	24	from	same	1040:1043	arg1	terms					1048:1052	terms	1048:1052	terms of which bacteria were producing amylases	1048:1094	Moreover, no two communities were the same in terms of which bacteria were producing amylases.
33298571	3	25	from	host	388:391	arg1	vagina					410:415	the vagina	406:415	the vagina	406:415	While α-amylase activity has been demonstrated in vaginal fluids, it is unclear whether α-amylases are produced solely by the host, bacteria in the vagina, or both.
33298571	4	26	theme	reproductive-age	468:483	arg1	women					485:489	23 reproductive-age women	465:489	23 reproductive-age women	465:489	We screened cervicovaginal mucus from 23 reproductive-age women, characterized the species composition of vaginal communities, measured vaginal pH, and determined levels of amylase activity, glycogen, and lactic acid.
33298571	11	27	theme	glycogen	1443:1450	arg1	levels					1412:1417	the levels	1408:1417	the levels of amylase activity and glycogen	1408:1450	Finally, the levels of amylase activity and glycogen were only weakly associated.
33298571	1	28	theme	species	57:63	arg1	Dominance					30:38	Dominance	30:38	Dominance of Lactobacillus species in vaginal communities	30:86	Dominance of Lactobacillus species in vaginal communities is a hallmark of healthy conditions in the female genital tract.
33298571	1	28	theme	species	57:63	arg1	hallmark					93:100	a hallmark	91:100	a hallmark of healthy conditions in the female genital tract	91:150	Dominance of Lactobacillus species in vaginal communities is a hallmark of healthy conditions in the female genital tract.
33298571	9	29	theme	amylase	1267:1273	arg1	activity					1275:1282	amylase activity	1267:1282	amylase activity	1267:1282	Although we detected bacterial amylases in vaginal fluids, there was no clear association between the bacterial species that was dominant in a community and the level of amylase activity.
33298571	1	30	theme	vaginal	68:74	arg1	communities					76:86	vaginal communities	68:86	vaginal communities	68:86	Dominance of Lactobacillus species in vaginal communities is a hallmark of healthy conditions in the female genital tract.
33298571	4	31	theme	cervicovaginal	439:452	arg1	mucus					454:458	cervicovaginal mucus	439:458	cervicovaginal mucus from 23 reproductive-age women	439:489	We screened cervicovaginal mucus from 23 reproductive-age women, characterized the species composition of vaginal communities, measured vaginal pH, and determined levels of amylase activity, glycogen, and lactic acid.
33298571	3	32	dep	host	388:391	arg1	the					384:386	the	384:386	the	384:386	While α-amylase activity has been demonstrated in vaginal fluids, it is unclear whether α-amylases are produced solely by the host, bacteria in the vagina, or both.
33298571	13	33	theme	multiple	1773:1780	arg1	bacteria					1782:1789	multiple bacteria	1773:1789	multiple bacteria in the vaginal community	1773:1814	This work also suggests that the link between glycogen, amylase, and Lactobacillus in the vagina is complex.IMPORTANCE In this study, we show that multiple bacteria in the vaginal community produce amylases that hydrolyze glycogen into simpler sugars (i.e., maltose and maltotriose).
33298571	5	34	theme	metagenomic	759:769	arg1	analyses					785:792	metagenomic and proteomic analyses	759:792	metagenomic and proteomic analyses	759:792	Based on differences in these measured variables, one sample from each of four individual donors was selected for metagenomic and proteomic analyses.
33298571	5	35	from	each	711:714	arg1	sample					699:704	one sample	695:704	one sample from each of four individual donors	695:740	Based on differences in these measured variables, one sample from each of four individual donors was selected for metagenomic and proteomic analyses.
33298571	12	36	theme	bacterial	1553:1561	arg1	origins					1573:1579	both bacterial and human origins	1548:1579	both bacterial and human origins	1548:1579	Our findings show, for the first time, that multiple amylases from both bacterial and human origins can be present simultaneously in the vagina.
33298571	14	37	theme	bacterial	1960:1968	arg1	populations					1970:1980	bacterial populations	1960:1980	bacterial populations	1960:1980	These sugars serve as "common goods" that sustain bacterial populations in vaginal communities.
33298571	5	38	theme	proteomic	775:783	arg1	analyses					785:792	metagenomic and proteomic analyses	759:792	metagenomic and proteomic analyses	759:792	Based on differences in these measured variables, one sample from each of four individual donors was selected for metagenomic and proteomic analyses.
33298571	13	39	dep	complex.IMPORTANCE	1726:1743	arg1	show					1763:1766	show	1763:1766	show that multiple bacteria in the vaginal community produce amylases that hydrolyze glycogen into simpler sugars (i.e., maltose and maltotriose)	1763:1907	This work also suggests that the link between glycogen, amylase, and Lactobacillus in the vagina is complex.IMPORTANCE In this study, we show that multiple bacteria in the vaginal community produce amylases that hydrolyze glycogen into simpler sugars (i.e., maltose and maltotriose).
33298571	15	40	located	observed	2042:2049	arg2	changes					2025:2031	the temporal changes	2012:2031	the temporal changes that are observed in the human vaginal microbiome	2012:2081	Given the temporal changes that are observed in the human vaginal microbiome, we expect the kinds of bacterial amylases produced will also vary over time.
33298571	15	40	located	observed	2042:2049	arg1	microbiome					2072:2081	the human vaginal microbiome	2054:2081	the human vaginal microbiome	2054:2081	Given the temporal changes that are observed in the human vaginal microbiome, we expect the kinds of bacterial amylases produced will also vary over time.
33298571	12	41	theme	human	1567:1571	arg1	origins					1573:1579	both bacterial and human origins	1548:1579	both bacterial and human origins	1548:1579	Our findings show, for the first time, that multiple amylases from both bacterial and human origins can be present simultaneously in the vagina.
33298571	4	42	theme	vaginal	533:539	arg1	communities					541:551	vaginal communities	533:551	vaginal communities	533:551	We screened cervicovaginal mucus from 23 reproductive-age women, characterized the species composition of vaginal communities, measured vaginal pH, and determined levels of amylase activity, glycogen, and lactic acid.
33298571	6	43	theme	bacterial	813:821	arg1	amylases					823:830	eight putative bacterial amylases	798:830	eight putative bacterial amylases identified in the assembled bacterial metagenomes	798:880	Of eight putative bacterial amylases identified in the assembled bacterial metagenomes, we detected four in vaginal fluids.
33298571	15	44	theme	temporal	2016:2023	arg1	changes					2025:2031	the temporal changes	2012:2031	the temporal changes that are observed in the human vaginal microbiome	2012:2081	Given the temporal changes that are observed in the human vaginal microbiome, we expect the kinds of bacterial amylases produced will also vary over time.
33298571	14	45	theme	goods	1940:1944	arg1	"					1945:1945	"common goods"	1932:1945	"common goods" that sustain bacterial populations in vaginal communities	1932:2003	These sugars serve as "common goods" that sustain bacterial populations in vaginal communities.
33298571	15	46	theme	amylases	2117:2124	arg1	kinds					2098:2102	the kinds	2094:2102	the kinds of bacterial amylases produced	2094:2133	Given the temporal changes that are observed in the human vaginal microbiome, we expect the kinds of bacterial amylases produced will also vary over time.
33298571	16	47	theme	vaginal	2276:2282	arg1	community					2294:2302	the vaginal bacterial community	2272:2302	the vaginal bacterial community	2272:2302	These differences influence the pool of resources that are broadly shared and shape the species composition of the vaginal bacterial community.
33298571	1	48	from	hallmark	93:100	arg1	tract					146:150	the female genital tract	127:150	the female genital tract	127:150	Dominance of Lactobacillus species in vaginal communities is a hallmark of healthy conditions in the female genital tract.
33298571	9	49	theme	bacterial	1118:1126	arg1	amylases					1128:1135	bacterial amylases	1118:1135	bacterial amylases	1118:1135	Although we detected bacterial amylases in vaginal fluids, there was no clear association between the bacterial species that was dominant in a community and the level of amylase activity.
33298571	0	50	dep	Amylases	0:7	arg1	Vagina					22:27	the Human Vagina	12:27	the Human Vagina	12:27	Amylases in the Human Vagina.
33298571	4	51	from	women	485:489	arg1	mucus					454:458	cervicovaginal mucus	439:458	cervicovaginal mucus from 23 reproductive-age women	439:489	We screened cervicovaginal mucus from 23 reproductive-age women, characterized the species composition of vaginal communities, measured vaginal pH, and determined levels of amylase activity, glycogen, and lactic acid.
33298571	12	52	attach	present	1588:1594	arg2	amylases					1534:1541	multiple amylases	1525:1541	multiple amylases from both bacterial and human origins	1525:1579	Our findings show, for the first time, that multiple amylases from both bacterial and human origins can be present simultaneously in the vagina.
33298571	12	52	attach	present	1588:1594	arg1	vagina					1618:1623	the vagina	1614:1623	the vagina	1614:1623	Our findings show, for the first time, that multiple amylases from both bacterial and human origins can be present simultaneously in the vagina.
33298571	10	53	theme	human	1339:1343	arg1	α-amylase					1345:1353	human α-amylase	1339:1353	human α-amylase	1339:1353	This association was likely masked by the presence of human α-amylase, which was also detected in vaginal fluids.
33298571	5	54	from	differences	654:664	arg1	variables					684:692	these measured variables	669:692	these measured variables	669:692	Based on differences in these measured variables, one sample from each of four individual donors was selected for metagenomic and proteomic analyses.
33298571	6	55	theme	bacterial	860:868	arg1	metagenomes					870:880	the assembled bacterial metagenomes	846:880	the assembled bacterial metagenomes	846:880	Of eight putative bacterial amylases identified in the assembled bacterial metagenomes, we detected four in vaginal fluids.
33298571	4	56	theme	vaginal	563:569	arg1	pH					571:572	vaginal pH	563:572	vaginal pH	563:572	We screened cervicovaginal mucus from 23 reproductive-age women, characterized the species composition of vaginal communities, measured vaginal pH, and determined levels of amylase activity, glycogen, and lactic acid.
33298571	15	57	theme	human	2058:2062	arg1	microbiome					2072:2081	the human vaginal microbiome	2054:2081	the human vaginal microbiome	2054:2081	Given the temporal changes that are observed in the human vaginal microbiome, we expect the kinds of bacterial amylases produced will also vary over time.
33298571	12	58	from	origins	1573:1579	arg1	amylases					1534:1541	multiple amylases	1525:1541	multiple amylases from both bacterial and human origins	1525:1579	Our findings show, for the first time, that multiple amylases from both bacterial and human origins can be present simultaneously in the vagina.
33298571	4	59	theme	glycogen	618:625	arg1	levels					590:595	levels	590:595	levels of amylase activity, glycogen, and lactic acid	590:642	We screened cervicovaginal mucus from 23 reproductive-age women, characterized the species composition of vaginal communities, measured vaginal pH, and determined levels of amylase activity, glycogen, and lactic acid.
33298571	12	60	theme	multiple	1525:1532	arg1	amylases					1534:1541	multiple amylases	1525:1541	multiple amylases from both bacterial and human origins	1525:1579	Our findings show, for the first time, that multiple amylases from both bacterial and human origins can be present simultaneously in the vagina.
33298571	7	61	from	bacteria	959:966	arg1	communities					989:999	different vaginal communities	971:999	different vaginal communities	971:999	These amylases were produced by various bacteria in different vaginal communities.
33298571	13	62	theme	simpler	1862:1868	arg1	sugars					1870:1875	simpler sugars	1862:1875	simpler sugars (i.e., maltose and maltotriose)	1862:1907	This work also suggests that the link between glycogen, amylase, and Lactobacillus in the vagina is complex.IMPORTANCE In this study, we show that multiple bacteria in the vaginal community produce amylases that hydrolyze glycogen into simpler sugars (i.e., maltose and maltotriose).
33298571	3	63	theme	α-amylase	268:276	arg1	activity					278:285	α-amylase activity	268:285	α-amylase activity	268:285	While α-amylase activity has been demonstrated in vaginal fluids, it is unclear whether α-amylases are produced solely by the host, bacteria in the vagina, or both.
33298571	12	64	theme	first	1508:1512	arg1	time					1514:1517	the first time	1504:1517	the first time	1504:1517	Our findings show, for the first time, that multiple amylases from both bacterial and human origins can be present simultaneously in the vagina.
33298571	4	65	theme	activity	608:615	arg1	levels					590:595	levels	590:595	levels of amylase activity, glycogen, and lactic acid	590:642	We screened cervicovaginal mucus from 23 reproductive-age women, characterized the species composition of vaginal communities, measured vaginal pH, and determined levels of amylase activity, glycogen, and lactic acid.
33298571	9	66	from	community	1240:1248	arg1	species					1209:1215	the bacterial species	1195:1215	the bacterial species that was dominant in a community	1195:1248	Although we detected bacterial amylases in vaginal fluids, there was no clear association between the bacterial species that was dominant in a community and the level of amylase activity.
33298571	9	66	from	community	1240:1248	arg1	dominant					1226:1233	dominant	1226:1233	dominant	1226:1233	Although we detected bacterial amylases in vaginal fluids, there was no clear association between the bacterial species that was dominant in a community and the level of amylase activity.
33298571	9	66	from	community	1240:1248	arg1	level					1258:1262	the level	1254:1262	the level of amylase activity	1254:1282	Although we detected bacterial amylases in vaginal fluids, there was no clear association between the bacterial species that was dominant in a community and the level of amylase activity.
33298571	11	67	theme	amylase	1422:1428	arg1	activity					1430:1437	amylase activity	1422:1437	amylase activity	1422:1437	Finally, the levels of amylase activity and glycogen were only weakly associated.
33298571	16	68	theme	species	2249:2255	arg1	composition					2257:2267	the species composition	2245:2267	the species composition of the vaginal bacterial community	2245:2302	These differences influence the pool of resources that are broadly shared and shape the species composition of the vaginal bacterial community.
33298571	7	69	theme	different	971:979	arg1	communities					989:999	different vaginal communities	971:999	different vaginal communities	971:999	These amylases were produced by various bacteria in different vaginal communities.
33298571	1	70	theme	conditions	113:122	arg1	Dominance					30:38	Dominance	30:38	Dominance of Lactobacillus species in vaginal communities	30:86	Dominance of Lactobacillus species in vaginal communities is a hallmark of healthy conditions in the female genital tract.
33298571	1	70	theme	conditions	113:122	arg1	hallmark					93:100	a hallmark	91:100	a hallmark of healthy conditions in the female genital tract	91:150	Dominance of Lactobacillus species in vaginal communities is a hallmark of healthy conditions in the female genital tract.
33298571	13	71	theme	vaginal	1798:1804	arg1	community					1806:1814	the vaginal community	1794:1814	the vaginal community	1794:1814	This work also suggests that the link between glycogen, amylase, and Lactobacillus in the vagina is complex.IMPORTANCE In this study, we show that multiple bacteria in the vaginal community produce amylases that hydrolyze glycogen into simpler sugars (i.e., maltose and maltotriose).
33298571	4	72	theme	acid	639:642	arg1	levels					590:595	levels	590:595	levels of amylase activity, glycogen, and lactic acid	590:642	We screened cervicovaginal mucus from 23 reproductive-age women, characterized the species composition of vaginal communities, measured vaginal pH, and determined levels of amylase activity, glycogen, and lactic acid.
33298571	1	73	theme	genital	138:144	arg1	tract					146:150	the female genital tract	127:150	the female genital tract	127:150	Dominance of Lactobacillus species in vaginal communities is a hallmark of healthy conditions in the female genital tract.
33298571	3	74	theme	vaginal	312:318	arg1	fluids					320:325	vaginal fluids	312:325	vaginal fluids	312:325	While α-amylase activity has been demonstrated in vaginal fluids, it is unclear whether α-amylases are produced solely by the host, bacteria in the vagina, or both.
33298571	10	75	theme	vaginal	1383:1389	arg1	fluids					1391:1396	vaginal fluids	1383:1396	vaginal fluids	1383:1396	This association was likely masked by the presence of human α-amylase, which was also detected in vaginal fluids.
33298571	1	76	theme	Lactobacillus	43:55	arg1	species					57:63	Lactobacillus species	43:63	Lactobacillus species	43:63	Dominance of Lactobacillus species in vaginal communities is a hallmark of healthy conditions in the female genital tract.
33298571	13	77	from	amylase	1682:1688	arg1	vagina					1716:1721	the vagina	1712:1721	the vagina	1712:1721	This work also suggests that the link between glycogen, amylase, and Lactobacillus in the vagina is complex.IMPORTANCE In this study, we show that multiple bacteria in the vaginal community produce amylases that hydrolyze glycogen into simpler sugars (i.e., maltose and maltotriose).
33298571	13	78	from	bacteria	1782:1789	arg1	community					1806:1814	the vaginal community	1794:1814	the vaginal community	1794:1814	This work also suggests that the link between glycogen, amylase, and Lactobacillus in the vagina is complex.IMPORTANCE In this study, we show that multiple bacteria in the vaginal community produce amylases that hydrolyze glycogen into simpler sugars (i.e., maltose and maltotriose).
33298571	13	79	dep	maltose	1884:1890	arg1	i.e.					1878:1881	i.e.	1878:1881	i.e.	1878:1881	This work also suggests that the link between glycogen, amylase, and Lactobacillus in the vagina is complex.IMPORTANCE In this study, we show that multiple bacteria in the vaginal community produce amylases that hydrolyze glycogen into simpler sugars (i.e., maltose and maltotriose).
33298571	14	80	theme	vaginal	1985:1991	arg1	communities					1993:2003	vaginal communities	1985:2003	vaginal communities	1985:2003	These sugars serve as "common goods" that sustain bacterial populations in vaginal communities.
33298571	9	81	theme	activity	1275:1282	arg1	species					1209:1215	the bacterial species	1195:1215	the bacterial species that was dominant in a community	1195:1248	Although we detected bacterial amylases in vaginal fluids, there was no clear association between the bacterial species that was dominant in a community and the level of amylase activity.
33298571	9	81	theme	activity	1275:1282	arg1	dominant					1226:1233	dominant	1226:1233	dominant	1226:1233	Although we detected bacterial amylases in vaginal fluids, there was no clear association between the bacterial species that was dominant in a community and the level of amylase activity.
33298571	9	81	theme	activity	1275:1282	arg1	level					1258:1262	the level	1254:1262	the level of amylase activity	1254:1282	Although we detected bacterial amylases in vaginal fluids, there was no clear association between the bacterial species that was dominant in a community and the level of amylase activity.
33298571	0	82	theme	Human	16:20	arg1	Vagina					22:27	the Human Vagina	12:27	the Human Vagina	12:27	Amylases in the Human Vagina.
33298571	16	83	theme	community	2294:2302	arg1	composition					2257:2267	the species composition	2245:2267	the species composition of the vaginal bacterial community	2245:2302	These differences influence the pool of resources that are broadly shared and shape the species composition of the vaginal bacterial community.
33298571	4	84	theme	communities	541:551	arg1	composition					518:528	the species composition	506:528	the species composition of vaginal communities	506:551	We screened cervicovaginal mucus from 23 reproductive-age women, characterized the species composition of vaginal communities, measured vaginal pH, and determined levels of amylase activity, glycogen, and lactic acid.
33298571	5	85	theme	individual	724:733	arg1	donors					735:740	four individual donors	719:740	four individual donors	719:740	Based on differences in these measured variables, one sample from each of four individual donors was selected for metagenomic and proteomic analyses.
33298571	2	86	theme	Key	153:155	arg1	nutrients					157:165	Key nutrients	153:165	Key nutrients for lactobacilli	153:182	Key nutrients for lactobacilli include sugars produced when glycogen is degraded by α-amylase in the vagina.
33298571	6	87	theme	putative	804:811	arg1	amylases					823:830	eight putative bacterial amylases	798:830	eight putative bacterial amylases identified in the assembled bacterial metagenomes	798:880	Of eight putative bacterial amylases identified in the assembled bacterial metagenomes, we detected four in vaginal fluids.
33298571	4	88	theme	species	510:516	arg1	composition					518:528	the species composition	506:528	the species composition of vaginal communities	506:551	We screened cervicovaginal mucus from 23 reproductive-age women, characterized the species composition of vaginal communities, measured vaginal pH, and determined levels of amylase activity, glycogen, and lactic acid.
33298571	14	89	theme	common	1933:1938	arg1	"					1945:1945	"common goods"	1932:1945	"common goods" that sustain bacterial populations in vaginal communities	1932:2003	These sugars serve as "common goods" that sustain bacterial populations in vaginal communities.
33298571	16	90	theme	bacterial	2284:2292	arg1	community					2294:2302	the vaginal bacterial community	2272:2302	the vaginal bacterial community	2272:2302	These differences influence the pool of resources that are broadly shared and shape the species composition of the vaginal bacterial community.
33298571	3	91	from	bacteria	394:401	arg1	vagina					410:415	the vagina	406:415	the vagina	406:415	While α-amylase activity has been demonstrated in vaginal fluids, it is unclear whether α-amylases are produced solely by the host, bacteria in the vagina, or both.
33298571	9	92	from	dominant	1226:1233	arg1	community					1240:1248	a community	1238:1248	a community	1238:1248	Although we detected bacterial amylases in vaginal fluids, there was no clear association between the bacterial species that was dominant in a community and the level of amylase activity.
33298571	12	93	located	present	1588:1594	arg2	amylases					1534:1541	multiple amylases	1525:1541	multiple amylases from both bacterial and human origins	1525:1579	Our findings show, for the first time, that multiple amylases from both bacterial and human origins can be present simultaneously in the vagina.
33298571	12	93	located	present	1588:1594	arg1	vagina					1618:1623	the vagina	1614:1623	the vagina	1614:1623	Our findings show, for the first time, that multiple amylases from both bacterial and human origins can be present simultaneously in the vagina.
33773460	2	0	theme	propolis	344:351	arg1	extract					353:359	the green propolis extract	334:359	the green propolis extract in shelf-life, physical-chemical properties, microbiological properties and sensory acceptance of Colossoma macropomum fillets	334:486	The objective of this work was to evaluate the effect of a sodium alginate bilayer coating incorporated to the green propolis extract in shelf-life, physical-chemical properties, microbiological properties and sensory acceptance of Colossoma macropomum fillets.
33773460	6	1	theme	propolis	924:931	arg1	extracts					933:940	green propolis extracts	918:940	green propolis extracts	918:940	Cyclolaudenol was the major constituent of the GPE and it is described for the first time in green propolis extracts.
33773460	7	2	theme	sodium	982:987	arg1	coating					1006:1012	the sodium alginate bilayer coating	978:1012	the sodium alginate bilayer coating	978:1012	C. macropomum fillets treated with the sodium alginate bilayer coating showed high sensory acceptance, reduced microbial deterioration and extended shelf-life (up to 11 days) during cold storage.
33773460	2	3	theme	green	338:342	arg1	extract					353:359	the green propolis extract	334:359	the green propolis extract in shelf-life, physical-chemical properties, microbiological properties and sensory acceptance of Colossoma macropomum fillets	334:486	The objective of this work was to evaluate the effect of a sodium alginate bilayer coating incorporated to the green propolis extract in shelf-life, physical-chemical properties, microbiological properties and sensory acceptance of Colossoma macropomum fillets.
33773460	6	4	theme	green	918:922	arg1	extracts					933:940	green propolis extracts	918:940	green propolis extracts	918:940	Cyclolaudenol was the major constituent of the GPE and it is described for the first time in green propolis extracts.
33773460	6	5	theme	major	847:851	arg1	Cyclolaudenol					825:837	Cyclolaudenol	825:837	Cyclolaudenol	825:837	Cyclolaudenol was the major constituent of the GPE and it is described for the first time in green propolis extracts.
33773460	6	5	theme	major	847:851	arg1	constituent					853:863	the major constituent	843:863	the major constituent of the GPE	843:874	Cyclolaudenol was the major constituent of the GPE and it is described for the first time in green propolis extracts.
33773460	3	6	theme	antibacterial	560:572	arg1	activities					574:583	the antioxidant and antibacterial activities	540:583	activities	574:583	Additionally, the chemical composition, along with the antioxidant and antibacterial activities of Brazilian green propolis extract (GPE) were investigated.
33773460	0	7	theme	powerful	80:87	arg1	tool					89:92	a powerful tool	78:92	a powerful tool to extend Colossoma macropomum fillet shelf-life	78:141	A sodium alginate bilayer coating incorporated with green propolis extract as a powerful tool to extend Colossoma macropomum fillet shelf-life.
33773460	3	8	theme	propolis	604:611	arg1	GPE					622:624	GPE	622:624	GPE	622:624	Additionally, the chemical composition, along with the antioxidant and antibacterial activities of Brazilian green propolis extract (GPE) were investigated.
33773460	3	8	theme	propolis	604:611	arg1	extract					613:619	Brazilian green propolis extract	588:619	Brazilian green propolis extract (GPE)	588:625	Additionally, the chemical composition, along with the antioxidant and antibacterial activities of Brazilian green propolis extract (GPE) were investigated.
33773460	3	9	theme	antioxidant	544:554	arg1	activities					574:583	the antioxidant and antibacterial activities	540:583	activities	574:583	Additionally, the chemical composition, along with the antioxidant and antibacterial activities of Brazilian green propolis extract (GPE) were investigated.
33773460	1	10	theme	great	171:175	arg1	losses					186:191	great economic losses	171:191	great economic losses	171:191	Fish deterioration imposes great economic losses and serious human health hazards.
33773460	6	11	from	time	910:913	arg1	extracts					933:940	green propolis extracts	918:940	green propolis extracts	918:940	Cyclolaudenol was the major constituent of the GPE and it is described for the first time in green propolis extracts.
33773460	6	12	theme	first	904:908	arg1	time					910:913	the first time	900:913	the first time in green propolis extracts	900:940	Cyclolaudenol was the major constituent of the GPE and it is described for the first time in green propolis extracts.
33773460	7	13	theme	cold	1125:1128	arg1	storage					1130:1136	cold storage	1125:1136	cold storage	1125:1136	C. macropomum fillets treated with the sodium alginate bilayer coating showed high sensory acceptance, reduced microbial deterioration and extended shelf-life (up to 11 days) during cold storage.
33773460	7	14	theme	high	1021:1024	arg1	acceptance					1034:1043	high sensory acceptance	1021:1043	high sensory acceptance	1021:1043	C. macropomum fillets treated with the sodium alginate bilayer coating showed high sensory acceptance, reduced microbial deterioration and extended shelf-life (up to 11 days) during cold storage.
33773460	1	15	theme	economic	177:184	arg1	losses					186:191	great economic losses	171:191	great economic losses	171:191	Fish deterioration imposes great economic losses and serious human health hazards.
33773460	2	16	theme	Colossoma	459:467	arg1	fillets					480:486	Colossoma macropomum fillets	459:486	Colossoma macropomum fillets	459:486	The objective of this work was to evaluate the effect of a sodium alginate bilayer coating incorporated to the green propolis extract in shelf-life, physical-chemical properties, microbiological properties and sensory acceptance of Colossoma macropomum fillets.
33773460	4	17	theme	antibacterial	683:695	arg1	activities					697:706	promising antioxidant and antibacterial activities	657:706	promising antioxidant and antibacterial activities	657:706	GPE showed promising antioxidant and antibacterial activities.
33773460	2	18	theme	bilayer	302:308	arg1	effect					274:279	the effect	270:279	the effect of a sodium alginate bilayer coating incorporated to the green propolis extract in shelf-life, physical-chemical properties, microbiological properties and sensory acceptance of Colossoma macropomum fillets	270:486	The objective of this work was to evaluate the effect of a sodium alginate bilayer coating incorporated to the green propolis extract in shelf-life, physical-chemical properties, microbiological properties and sensory acceptance of Colossoma macropomum fillets.
33773460	7	19	theme	extended	1082:1089	arg1	shelf-life					1091:1100	extended shelf-life	1082:1100	extended shelf-life (up to 11 days)	1082:1116	C. macropomum fillets treated with the sodium alginate bilayer coating showed high sensory acceptance, reduced microbial deterioration and extended shelf-life (up to 11 days) during cold storage.
33773460	7	20	theme	macropomum	946:955	arg1	fillets					957:963	C. macropomum fillets	943:963	C. macropomum fillets treated with the sodium alginate bilayer coating	943:1012	C. macropomum fillets treated with the sodium alginate bilayer coating showed high sensory acceptance, reduced microbial deterioration and extended shelf-life (up to 11 days) during cold storage.
33773460	7	21	theme	C.	943:944	arg1	fillets					957:963	C. macropomum fillets	943:963	C. macropomum fillets treated with the sodium alginate bilayer coating	943:1012	C. macropomum fillets treated with the sodium alginate bilayer coating showed high sensory acceptance, reduced microbial deterioration and extended shelf-life (up to 11 days) during cold storage.
33773460	2	22	theme	alginate	293:300	arg1	bilayer					302:308	a sodium alginate bilayer	284:308	a sodium alginate bilayer coating incorporated to the green propolis extract in shelf-life, physical-chemical properties, microbiological properties and sensory acceptance of Colossoma macropomum fillets	284:486	The objective of this work was to evaluate the effect of a sodium alginate bilayer coating incorporated to the green propolis extract in shelf-life, physical-chemical properties, microbiological properties and sensory acceptance of Colossoma macropomum fillets.
33773460	0	23	theme	bilayer	18:24	arg1	coating					26:32	A sodium alginate bilayer coating	0:32	A sodium alginate bilayer coating	0:32	A sodium alginate bilayer coating incorporated with green propolis extract as a powerful tool to extend Colossoma macropomum fillet shelf-life.
33773460	0	24	theme	macropomum	114:123	arg1	shelf-life					132:141	Colossoma macropomum fillet shelf-life	104:141	Colossoma macropomum fillet shelf-life	104:141	A sodium alginate bilayer coating incorporated with green propolis extract as a powerful tool to extend Colossoma macropomum fillet shelf-life.
33773460	2	25	from	extract	353:359	arg1	shelf-life					364:373	shelf-life	364:373	shelf-life	364:373	The objective of this work was to evaluate the effect of a sodium alginate bilayer coating incorporated to the green propolis extract in shelf-life, physical-chemical properties, microbiological properties and sensory acceptance of Colossoma macropomum fillets.
33773460	2	25	from	extract	353:359	arg1	properties					394:403	physical-chemical properties	376:403	physical-chemical properties	376:403	The objective of this work was to evaluate the effect of a sodium alginate bilayer coating incorporated to the green propolis extract in shelf-life, physical-chemical properties, microbiological properties and sensory acceptance of Colossoma macropomum fillets.
33773460	2	25	from	extract	353:359	arg1	properties					422:431	microbiological properties	406:431	microbiological properties	406:431	The objective of this work was to evaluate the effect of a sodium alginate bilayer coating incorporated to the green propolis extract in shelf-life, physical-chemical properties, microbiological properties and sensory acceptance of Colossoma macropomum fillets.
33773460	2	25	from	extract	353:359	arg1	acceptance					445:454	sensory acceptance	437:454	sensory acceptance of Colossoma macropomum fillets	437:486	The objective of this work was to evaluate the effect of a sodium alginate bilayer coating incorporated to the green propolis extract in shelf-life, physical-chemical properties, microbiological properties and sensory acceptance of Colossoma macropomum fillets.
33773460	2	26	theme	sodium	286:291	arg1	bilayer					302:308	a sodium alginate bilayer	284:308	a sodium alginate bilayer coating incorporated to the green propolis extract in shelf-life, physical-chemical properties, microbiological properties and sensory acceptance of Colossoma macropomum fillets	284:486	The objective of this work was to evaluate the effect of a sodium alginate bilayer coating incorporated to the green propolis extract in shelf-life, physical-chemical properties, microbiological properties and sensory acceptance of Colossoma macropomum fillets.
33773460	1	27	theme	serious	197:203	arg1	hazards					218:224	serious human health hazards	197:224	serious human health hazards	197:224	Fish deterioration imposes great economic losses and serious human health hazards.
33773460	0	28	theme	alginate	9:16	arg1	coating					26:32	A sodium alginate bilayer coating	0:32	A sodium alginate bilayer coating	0:32	A sodium alginate bilayer coating incorporated with green propolis extract as a powerful tool to extend Colossoma macropomum fillet shelf-life.
33773460	0	29	theme	Colossoma	104:112	arg1	shelf-life					132:141	Colossoma macropomum fillet shelf-life	104:141	Colossoma macropomum fillet shelf-life	104:141	A sodium alginate bilayer coating incorporated with green propolis extract as a powerful tool to extend Colossoma macropomum fillet shelf-life.
33773460	3	30	theme	Brazilian	588:596	arg1	GPE					622:624	GPE	622:624	GPE	622:624	Additionally, the chemical composition, along with the antioxidant and antibacterial activities of Brazilian green propolis extract (GPE) were investigated.
33773460	3	30	theme	Brazilian	588:596	arg1	extract					613:619	Brazilian green propolis extract	588:619	Brazilian green propolis extract (GPE)	588:625	Additionally, the chemical composition, along with the antioxidant and antibacterial activities of Brazilian green propolis extract (GPE) were investigated.
33773460	1	31	theme	human	205:209	arg1	hazards					218:224	serious human health hazards	197:224	serious human health hazards	197:224	Fish deterioration imposes great economic losses and serious human health hazards.
33773460	8	32	theme	natural	1215:1221	arg1	preservative					1223:1234	a natural preservative	1213:1234	a natural preservative	1213:1234	Taken together, these results show that GPE can be a great alternative of a natural preservative for fish coating.
33773460	2	33	theme	microbiological	406:420	arg1	properties					422:431	microbiological properties	406:431	microbiological properties	406:431	The objective of this work was to evaluate the effect of a sodium alginate bilayer coating incorporated to the green propolis extract in shelf-life, physical-chemical properties, microbiological properties and sensory acceptance of Colossoma macropomum fillets.
33773460	8	34	theme	great	1192:1196	arg1	alternative					1198:1208	a great alternative	1190:1208	a great alternative of a natural preservative for fish coating	1190:1251	Taken together, these results show that GPE can be a great alternative of a natural preservative for fish coating.
33773460	8	34	theme	great	1192:1196	arg1	GPE					1179:1181	GPE	1179:1181	GPE	1179:1181	Taken together, these results show that GPE can be a great alternative of a natural preservative for fish coating.
33773460	1	35	theme	health	211:216	arg1	hazards					218:224	serious human health hazards	197:224	serious human health hazards	197:224	Fish deterioration imposes great economic losses and serious human health hazards.
33773460	8	36	theme	fish	1240:1243	arg1	coating					1245:1251	fish coating	1240:1251	fish coating	1240:1251	Taken together, these results show that GPE can be a great alternative of a natural preservative for fish coating.
33773460	0	37	theme	fillet	125:130	arg1	shelf-life					132:141	Colossoma macropomum fillet shelf-life	104:141	Colossoma macropomum fillet shelf-life	104:141	A sodium alginate bilayer coating incorporated with green propolis extract as a powerful tool to extend Colossoma macropomum fillet shelf-life.
33773460	4	38	theme	antioxidant	667:677	arg1	activities					697:706	promising antioxidant and antibacterial activities	657:706	promising antioxidant and antibacterial activities	657:706	GPE showed promising antioxidant and antibacterial activities.
33773460	2	39	theme	fillets	480:486	arg1	shelf-life					364:373	shelf-life	364:373	shelf-life	364:373	The objective of this work was to evaluate the effect of a sodium alginate bilayer coating incorporated to the green propolis extract in shelf-life, physical-chemical properties, microbiological properties and sensory acceptance of Colossoma macropomum fillets.
33773460	2	39	theme	fillets	480:486	arg1	properties					394:403	physical-chemical properties	376:403	physical-chemical properties	376:403	The objective of this work was to evaluate the effect of a sodium alginate bilayer coating incorporated to the green propolis extract in shelf-life, physical-chemical properties, microbiological properties and sensory acceptance of Colossoma macropomum fillets.
33773460	2	39	theme	fillets	480:486	arg1	properties					422:431	microbiological properties	406:431	microbiological properties	406:431	The objective of this work was to evaluate the effect of a sodium alginate bilayer coating incorporated to the green propolis extract in shelf-life, physical-chemical properties, microbiological properties and sensory acceptance of Colossoma macropomum fillets.
33773460	2	39	theme	fillets	480:486	arg1	acceptance					445:454	sensory acceptance	437:454	sensory acceptance of Colossoma macropomum fillets	437:486	The objective of this work was to evaluate the effect of a sodium alginate bilayer coating incorporated to the green propolis extract in shelf-life, physical-chemical properties, microbiological properties and sensory acceptance of Colossoma macropomum fillets.
33773460	0	40	theme	propolis	58:65	arg1	extract					67:73	green propolis extract	52:73	green propolis extract	52:73	A sodium alginate bilayer coating incorporated with green propolis extract as a powerful tool to extend Colossoma macropomum fillet shelf-life.
33773460	3	41	theme	extract	613:619	arg1	composition					516:526	the chemical composition	503:526	the chemical composition	503:526	Additionally, the chemical composition, along with the antioxidant and antibacterial activities of Brazilian green propolis extract (GPE) were investigated.
33773460	7	42	theme	reduced	1046:1052	arg1	deterioration					1064:1076	reduced microbial deterioration	1046:1076	reduced microbial deterioration	1046:1076	C. macropomum fillets treated with the sodium alginate bilayer coating showed high sensory acceptance, reduced microbial deterioration and extended shelf-life (up to 11 days) during cold storage.
33773460	3	43	theme	chemical	507:514	arg1	composition					516:526	the chemical composition	503:526	the chemical composition	503:526	Additionally, the chemical composition, along with the antioxidant and antibacterial activities of Brazilian green propolis extract (GPE) were investigated.
33773460	2	44	theme	macropomum	469:478	arg1	fillets					480:486	Colossoma macropomum fillets	459:486	Colossoma macropomum fillets	459:486	The objective of this work was to evaluate the effect of a sodium alginate bilayer coating incorporated to the green propolis extract in shelf-life, physical-chemical properties, microbiological properties and sensory acceptance of Colossoma macropomum fillets.
33773460	5	45	theme	gas	753:755	arg1	chromatography					757:770	gas chromatography	753:770	gas chromatography (GC-MS)	753:778	Twenty-seven metabolites were identified by gas chromatography (GC-MS), which mainly comprised terpenoids (52.14%).
33773460	5	45	theme	gas	753:755	arg1	GC-MS					773:777	GC-MS	773:777	GC-MS	773:777	Twenty-seven metabolites were identified by gas chromatography (GC-MS), which mainly comprised terpenoids (52.14%).
33773460	7	46	theme	sensory	1026:1032	arg1	acceptance					1034:1043	high sensory acceptance	1021:1043	high sensory acceptance	1021:1043	C. macropomum fillets treated with the sodium alginate bilayer coating showed high sensory acceptance, reduced microbial deterioration and extended shelf-life (up to 11 days) during cold storage.
33773460	3	47	dep	along	529:533	arg1	with					535:538	with	535:538	with	535:538	Additionally, the chemical composition, along with the antioxidant and antibacterial activities of Brazilian green propolis extract (GPE) were investigated.
33773460	3	48	theme	green	598:602	arg1	GPE					622:624	GPE	622:624	GPE	622:624	Additionally, the chemical composition, along with the antioxidant and antibacterial activities of Brazilian green propolis extract (GPE) were investigated.
33773460	3	48	theme	green	598:602	arg1	extract					613:619	Brazilian green propolis extract	588:619	Brazilian green propolis extract (GPE)	588:625	Additionally, the chemical composition, along with the antioxidant and antibacterial activities of Brazilian green propolis extract (GPE) were investigated.
33773460	7	49	theme	microbial	1054:1062	arg1	deterioration					1064:1076	reduced microbial deterioration	1046:1076	reduced microbial deterioration	1046:1076	C. macropomum fillets treated with the sodium alginate bilayer coating showed high sensory acceptance, reduced microbial deterioration and extended shelf-life (up to 11 days) during cold storage.
33773460	6	50	theme	GPE	872:874	arg1	Cyclolaudenol					825:837	Cyclolaudenol	825:837	Cyclolaudenol	825:837	Cyclolaudenol was the major constituent of the GPE and it is described for the first time in green propolis extracts.
33773460	6	50	theme	GPE	872:874	arg1	constituent					853:863	the major constituent	843:863	the major constituent of the GPE	843:874	Cyclolaudenol was the major constituent of the GPE and it is described for the first time in green propolis extracts.
33773460	7	51	theme	alginate	989:996	arg1	coating					1006:1012	the sodium alginate bilayer coating	978:1012	the sodium alginate bilayer coating	978:1012	C. macropomum fillets treated with the sodium alginate bilayer coating showed high sensory acceptance, reduced microbial deterioration and extended shelf-life (up to 11 days) during cold storage.
33773460	8	52	theme	preservative	1223:1234	arg1	alternative					1198:1208	a great alternative	1190:1208	a great alternative of a natural preservative for fish coating	1190:1251	Taken together, these results show that GPE can be a great alternative of a natural preservative for fish coating.
33773460	8	52	theme	preservative	1223:1234	arg1	GPE					1179:1181	GPE	1179:1181	GPE	1179:1181	Taken together, these results show that GPE can be a great alternative of a natural preservative for fish coating.
33773460	1	53	theme	Fish	144:147	arg1	deterioration					149:161	Fish deterioration	144:161	Fish deterioration	144:161	Fish deterioration imposes great economic losses and serious human health hazards.
33773460	7	54	dep	shelf-life	1091:1100	arg1	up					1103:1104	up	1103:1104	up to 11 days	1103:1115	C. macropomum fillets treated with the sodium alginate bilayer coating showed high sensory acceptance, reduced microbial deterioration and extended shelf-life (up to 11 days) during cold storage.
33773460	2	55	theme	work	249:252	arg1	objective					231:239	The objective	227:239	The objective of this work	227:252	The objective of this work was to evaluate the effect of a sodium alginate bilayer coating incorporated to the green propolis extract in shelf-life, physical-chemical properties, microbiological properties and sensory acceptance of Colossoma macropomum fillets.
33773460	4	56	theme	promising	657:665	arg1	activities					697:706	promising antioxidant and antibacterial activities	657:706	promising antioxidant and antibacterial activities	657:706	GPE showed promising antioxidant and antibacterial activities.
33773460	2	57	theme	physical-chemical	376:392	arg1	properties					394:403	physical-chemical properties	376:403	physical-chemical properties	376:403	The objective of this work was to evaluate the effect of a sodium alginate bilayer coating incorporated to the green propolis extract in shelf-life, physical-chemical properties, microbiological properties and sensory acceptance of Colossoma macropomum fillets.
33773460	0	58	theme	green	52:56	arg1	extract					67:73	green propolis extract	52:73	green propolis extract	52:73	A sodium alginate bilayer coating incorporated with green propolis extract as a powerful tool to extend Colossoma macropomum fillet shelf-life.
33773460	2	59	theme	sensory	437:443	arg1	acceptance					445:454	sensory acceptance	437:454	sensory acceptance of Colossoma macropomum fillets	437:486	The objective of this work was to evaluate the effect of a sodium alginate bilayer coating incorporated to the green propolis extract in shelf-life, physical-chemical properties, microbiological properties and sensory acceptance of Colossoma macropomum fillets.
33773460	7	60	theme	bilayer	998:1004	arg1	coating					1006:1012	the sodium alginate bilayer coating	978:1012	the sodium alginate bilayer coating	978:1012	C. macropomum fillets treated with the sodium alginate bilayer coating showed high sensory acceptance, reduced microbial deterioration and extended shelf-life (up to 11 days) during cold storage.
33773460	0	61	theme	sodium	2:7	arg1	coating					26:32	A sodium alginate bilayer coating	0:32	A sodium alginate bilayer coating	0:32	A sodium alginate bilayer coating incorporated with green propolis extract as a powerful tool to extend Colossoma macropomum fillet shelf-life.
33100848	4	0	theme	polysaccharides	617:631	arg1	potential					597:605	Antioxidant potential	585:605	Antioxidant potential of the GC polysaccharides	585:631	Antioxidant potential of the GC polysaccharides were performed by free radical DPPH scavenging, superoxide anion scavenging, hydroxyl radical scavenging, reducing power potential assay, and hydrogen peroxide method.
33100848	7	1	theme	essential	1125:1133	arg1	assays					1145:1150	essential inhibitor assays	1125:1150	essential inhibitor assays	1125:1150	Compared to the standard inhibitor, the GC polysaccharides showed essential inhibitor assays in a very dose dependent approach, and notable actions to scavenge reactive oxygen species (ROS) are also due to the large quantities of hydrophilic polyphenols.
33100848	3	2	theme	membrane	562:569	arg1	assay					494:498	agar diffusion assay	479:498	agar diffusion assay	479:498	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides were examined by means of agar diffusion assay, minimum inhibitory concentration (MIC), outer and inner cell membrane permeability.
33100848	3	2	theme	membrane	562:569	arg1	permeability					571:582	outer and inner cell membrane permeability	541:582	outer and inner cell membrane permeability	541:582	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides were examined by means of agar diffusion assay, minimum inhibitory concentration (MIC), outer and inner cell membrane permeability.
33100848	5	3	theme	cell	927:930	arg1	lines					932:936	human cervical (HeLa) and liver carcinoma (HepG2) cell lines	877:936	lines	932:936	Cytotoxicity potential of GC polysaccharides were evaluated by MTT assay in human cervical (HeLa) and liver carcinoma (HepG2) cell lines.
33100848	4	4	theme	anion	692:696	arg1	scavenging					698:707	superoxide anion scavenging	681:707	superoxide anion scavenging	681:707	Antioxidant potential of the GC polysaccharides were performed by free radical DPPH scavenging, superoxide anion scavenging, hydroxyl radical scavenging, reducing power potential assay, and hydrogen peroxide method.
33100848	3	5	theme	inner	551:555	arg1	assay					494:498	agar diffusion assay	479:498	agar diffusion assay	479:498	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides were examined by means of agar diffusion assay, minimum inhibitory concentration (MIC), outer and inner cell membrane permeability.
33100848	3	5	theme	inner	551:555	arg1	permeability					571:582	outer and inner cell membrane permeability	541:582	outer and inner cell membrane permeability	541:582	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides were examined by means of agar diffusion assay, minimum inhibitory concentration (MIC), outer and inner cell membrane permeability.
33100848	11	6	theme	natural	1789:1795	arg1	antioxidant					1797:1807	natural antioxidant	1789:1807	natural antioxidant	1789:1807	The findings obtained in this study indicate that GC polysaccharides has antibacterial and has a possible source of natural antioxidant and also has cytotoxic effect on different carcinoma cell lines.
33100848	2	7	theme	polysaccharides	331:345	arg1	potential					300:308	the antibacterial, anti-scavenging and cytotoxic potential	251:308	the antibacterial, anti-scavenging and cytotoxic potential of garden cress (GC) polysaccharides	251:345	The present investigation aimed to examine the antibacterial, anti-scavenging and cytotoxic potential of garden cress (GC) polysaccharides.
33100848	5	8	theme	liver	903:907	arg1	lines					932:936	human cervical (HeLa) and liver carcinoma (HepG2) cell lines	877:936	lines	932:936	Cytotoxicity potential of GC polysaccharides were evaluated by MTT assay in human cervical (HeLa) and liver carcinoma (HepG2) cell lines.
33100848	11	9	contain	has	1742:1744	arg2	antibacterial					1746:1758	antibacterial	1746:1758	antibacterial	1746:1758	The findings obtained in this study indicate that GC polysaccharides has antibacterial and has a possible source of natural antioxidant and also has cytotoxic effect on different carcinoma cell lines.
33100848	11	9	contain	has	1742:1744	arg1	polysaccharides					1726:1740	GC polysaccharides	1723:1740	GC polysaccharides	1723:1740	The findings obtained in this study indicate that GC polysaccharides has antibacterial and has a possible source of natural antioxidant and also has cytotoxic effect on different carcinoma cell lines.
33100848	4	10	theme	DPPH	664:667	arg1	scavenging					669:678	free radical DPPH scavenging	651:678	free radical DPPH scavenging	651:678	Antioxidant potential of the GC polysaccharides were performed by free radical DPPH scavenging, superoxide anion scavenging, hydroxyl radical scavenging, reducing power potential assay, and hydrogen peroxide method.
33100848	10	11	theme	anti-tumor	1597:1606	arg1	activity					1608:1615	higher anti-tumor activity	1590:1615	higher anti-tumor activity	1590:1615	GC polysaccharides at a dose of 500 µg ml-1 exhibited higher anti-tumor activity in both HeLa (65.33 ± 3.75%) and HepG2 (60.33 ± 3.48%).
33100848	5	12	theme	human	877:881	arg1	HeLa					893:896	human cervical (HeLa) and liver carcinoma (HepG2) cell lines	877:936	HeLa	893:896	Cytotoxicity potential of GC polysaccharides were evaluated by MTT assay in human cervical (HeLa) and liver carcinoma (HepG2) cell lines.
33100848	6	13	theme	polysaccharides	967:981	arg1	MIC					983:985	GC polysaccharides MIC	964:985	GC polysaccharides MIC	964:985	The findings showed that GC polysaccharides MIC were 1.06 and 0.56 mg mL-1 against E. coli and S. aureus, respectively.
33100848	2	14	theme	antibacterial	255:267	arg1	potential					300:308	the antibacterial, anti-scavenging and cytotoxic potential	251:308	the antibacterial, anti-scavenging and cytotoxic potential of garden cress (GC) polysaccharides	251:345	The present investigation aimed to examine the antibacterial, anti-scavenging and cytotoxic potential of garden cress (GC) polysaccharides.
33100848	4	15	theme	power	748:752	arg1	assay					764:768	reducing power potential assay	739:768	reducing power potential assay	739:768	Antioxidant potential of the GC polysaccharides were performed by free radical DPPH scavenging, superoxide anion scavenging, hydroxyl radical scavenging, reducing power potential assay, and hydrogen peroxide method.
33100848	8	16	theme	standard	1377:1384	arg1	agent					1412:1416	standard ascorbic acid antioxidant agent	1377:1416	standard ascorbic acid antioxidant agent	1377:1416	The IC50 values of all tested parameters were measured against standard ascorbic acid antioxidant agent.
33100848	2	17	theme	cytotoxic	290:298	arg1	potential					300:308	the antibacterial, anti-scavenging and cytotoxic potential	251:308	the antibacterial, anti-scavenging and cytotoxic potential of garden cress (GC) polysaccharides	251:345	The present investigation aimed to examine the antibacterial, anti-scavenging and cytotoxic potential of garden cress (GC) polysaccharides.
33100848	1	18	theme	composites	141:150	arg1	stock					127:131	an infinite stock	115:131	an infinite stock of drug composites with varying pharmacological and biological activities	115:205	Plants polysaccharides are an infinite stock of drug composites with varying pharmacological and biological activities.
33100848	1	18	theme	composites	141:150	arg1	polysaccharides					95:109	Plants polysaccharides	88:109	Plants polysaccharides	88:109	Plants polysaccharides are an infinite stock of drug composites with varying pharmacological and biological activities.
33100848	4	19	theme	radical	719:725	arg1	scavenging					727:736	hydroxyl radical scavenging	710:736	hydroxyl radical scavenging	710:736	Antioxidant potential of the GC polysaccharides were performed by free radical DPPH scavenging, superoxide anion scavenging, hydroxyl radical scavenging, reducing power potential assay, and hydrogen peroxide method.
33100848	11	20	theme	GC	1723:1724	arg1	polysaccharides					1726:1740	GC polysaccharides	1723:1740	GC polysaccharides	1723:1740	The findings obtained in this study indicate that GC polysaccharides has antibacterial and has a possible source of natural antioxidant and also has cytotoxic effect on different carcinoma cell lines.
33100848	0	21	theme	cress	65:69	arg1	activity					46:53	Anti-bacterial, anti-scavenging and cytotoxic activity	0:53	Anti-bacterial, anti-scavenging and cytotoxic activity of garden cress	0:69	Anti-bacterial, anti-scavenging and cytotoxic activity of garden cress polysaccharides.
33100848	2	22	theme	anti-scavenging	270:284	arg1	potential					300:308	the antibacterial, anti-scavenging and cytotoxic potential	251:308	the antibacterial, anti-scavenging and cytotoxic potential of garden cress (GC) polysaccharides	251:345	The present investigation aimed to examine the antibacterial, anti-scavenging and cytotoxic potential of garden cress (GC) polysaccharides.
33100848	1	23	theme	varying	157:163	arg1	activities					196:205	varying pharmacological and biological activities	157:205	varying pharmacological and biological activities	157:205	Plants polysaccharides are an infinite stock of drug composites with varying pharmacological and biological activities.
33100848	7	24	theme	oxygen	1228:1233	arg1	ROS					1244:1246	ROS	1244:1246	ROS	1244:1246	Compared to the standard inhibitor, the GC polysaccharides showed essential inhibitor assays in a very dose dependent approach, and notable actions to scavenge reactive oxygen species (ROS) are also due to the large quantities of hydrophilic polyphenols.
33100848	7	24	theme	oxygen	1228:1233	arg1	species					1235:1241	reactive oxygen species	1219:1241	reactive oxygen species (ROS)	1219:1247	Compared to the standard inhibitor, the GC polysaccharides showed essential inhibitor assays in a very dose dependent approach, and notable actions to scavenge reactive oxygen species (ROS) are also due to the large quantities of hydrophilic polyphenols.
33100848	2	25	theme	cress	320:324	arg1	polysaccharides					331:345	garden cress (GC) polysaccharides	313:345	garden cress (GC) polysaccharides	313:345	The present investigation aimed to examine the antibacterial, anti-scavenging and cytotoxic potential of garden cress (GC) polysaccharides.
33100848	3	26	theme	Escherichia	377:387	arg1	effects					366:372	The antibacterial effects	348:372	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides	348:451	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides were examined by means of agar diffusion assay, minimum inhibitory concentration (MIC), outer and inner cell membrane permeability.
33100848	8	27	theme	parameters	1344:1353	arg1	values					1323:1328	The IC50 values	1314:1328	The IC50 values of all tested parameters	1314:1353	The IC50 values of all tested parameters were measured against standard ascorbic acid antioxidant agent.
33100848	1	28	theme	Plants	88:93	arg1	stock					127:131	an infinite stock	115:131	an infinite stock of drug composites with varying pharmacological and biological activities	115:205	Plants polysaccharides are an infinite stock of drug composites with varying pharmacological and biological activities.
33100848	1	28	theme	Plants	88:93	arg1	polysaccharides					95:109	Plants polysaccharides	88:109	Plants polysaccharides	88:109	Plants polysaccharides are an infinite stock of drug composites with varying pharmacological and biological activities.
33100848	0	29	theme	Anti-bacterial	0:13	arg1	activity					46:53	Anti-bacterial, anti-scavenging and cytotoxic activity	0:53	Anti-bacterial, anti-scavenging and cytotoxic activity of garden cress	0:69	Anti-bacterial, anti-scavenging and cytotoxic activity of garden cress polysaccharides.
33100848	4	30	theme	peroxide	784:791	arg1	method					793:798	hydrogen peroxide method	775:798	hydrogen peroxide method	775:798	Antioxidant potential of the GC polysaccharides were performed by free radical DPPH scavenging, superoxide anion scavenging, hydroxyl radical scavenging, reducing power potential assay, and hydrogen peroxide method.
33100848	0	31	theme	anti-scavenging	16:30	arg1	activity					46:53	Anti-bacterial, anti-scavenging and cytotoxic activity	0:53	Anti-bacterial, anti-scavenging and cytotoxic activity of garden cress	0:69	Anti-bacterial, anti-scavenging and cytotoxic activity of garden cress polysaccharides.
33100848	1	32	theme	infinite	118:125	arg1	stock					127:131	an infinite stock	115:131	an infinite stock of drug composites with varying pharmacological and biological activities	115:205	Plants polysaccharides are an infinite stock of drug composites with varying pharmacological and biological activities.
33100848	1	32	theme	infinite	118:125	arg1	polysaccharides					95:109	Plants polysaccharides	88:109	Plants polysaccharides	88:109	Plants polysaccharides are an infinite stock of drug composites with varying pharmacological and biological activities.
33100848	7	33	theme	polyphenols	1301:1311	arg1	quantities					1275:1284	the large quantities	1265:1284	the large quantities of hydrophilic polyphenols	1265:1311	Compared to the standard inhibitor, the GC polysaccharides showed essential inhibitor assays in a very dose dependent approach, and notable actions to scavenge reactive oxygen species (ROS) are also due to the large quantities of hydrophilic polyphenols.
33100848	3	34	theme	polysaccharides	437:451	arg1	effects					366:372	The antibacterial effects	348:372	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides	348:451	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides were examined by means of agar diffusion assay, minimum inhibitory concentration (MIC), outer and inner cell membrane permeability.
33100848	4	35	theme	Antioxidant	585:595	arg1	potential					597:605	Antioxidant potential	585:605	Antioxidant potential of the GC polysaccharides	585:631	Antioxidant potential of the GC polysaccharides were performed by free radical DPPH scavenging, superoxide anion scavenging, hydroxyl radical scavenging, reducing power potential assay, and hydrogen peroxide method.
33100848	8	36	theme	acid	1395:1398	arg1	agent					1412:1416	standard ascorbic acid antioxidant agent	1377:1416	standard ascorbic acid antioxidant agent	1377:1416	The IC50 values of all tested parameters were measured against standard ascorbic acid antioxidant agent.
33100848	3	37	theme	agar	479:482	arg1	concentration					520:532	minimum inhibitory concentration	501:532	minimum inhibitory concentration (MIC)	501:538	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides were examined by means of agar diffusion assay, minimum inhibitory concentration (MIC), outer and inner cell membrane permeability.
33100848	3	37	theme	agar	479:482	arg1	assay					494:498	agar diffusion assay	479:498	agar diffusion assay	479:498	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides were examined by means of agar diffusion assay, minimum inhibitory concentration (MIC), outer and inner cell membrane permeability.
33100848	3	37	theme	agar	479:482	arg1	permeability					571:582	outer and inner cell membrane permeability	541:582	outer and inner cell membrane permeability	541:582	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides were examined by means of agar diffusion assay, minimum inhibitory concentration (MIC), outer and inner cell membrane permeability.
33100848	0	38	theme	cytotoxic	36:44	arg1	activity					46:53	Anti-bacterial, anti-scavenging and cytotoxic activity	0:53	Anti-bacterial, anti-scavenging and cytotoxic activity of garden cress	0:69	Anti-bacterial, anti-scavenging and cytotoxic activity of garden cress polysaccharides.
33100848	5	39	theme	GC	827:828	arg1	polysaccharides					830:844	GC polysaccharides	827:844	GC polysaccharides	827:844	Cytotoxicity potential of GC polysaccharides were evaluated by MTT assay in human cervical (HeLa) and liver carcinoma (HepG2) cell lines.
33100848	10	40	from	dose	1560:1563	arg1	polysaccharides					1539:1553	GC polysaccharides	1536:1553	GC polysaccharides at a dose of 500 µg ml-1	1536:1578	GC polysaccharides at a dose of 500 µg ml-1 exhibited higher anti-tumor activity in both HeLa (65.33 ± 3.75%) and HepG2 (60.33 ± 3.48%).
33100848	3	41	dep	Escherichia	377:387	arg1	coli					389:392	coli	389:392	coli	389:392	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides were examined by means of agar diffusion assay, minimum inhibitory concentration (MIC), outer and inner cell membrane permeability.
33100848	11	42	theme	different	1842:1850	arg1	lines					1867:1871	different carcinoma cell lines	1842:1871	different carcinoma cell lines	1842:1871	The findings obtained in this study indicate that GC polysaccharides has antibacterial and has a possible source of natural antioxidant and also has cytotoxic effect on different carcinoma cell lines.
33100848	7	43	theme	large	1269:1273	arg1	quantities					1275:1284	the large quantities	1265:1284	the large quantities of hydrophilic polyphenols	1265:1311	Compared to the standard inhibitor, the GC polysaccharides showed essential inhibitor assays in a very dose dependent approach, and notable actions to scavenge reactive oxygen species (ROS) are also due to the large quantities of hydrophilic polyphenols.
33100848	3	44	theme	minimum	501:507	arg1	concentration					520:532	minimum inhibitory concentration	501:532	minimum inhibitory concentration (MIC)	501:538	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides were examined by means of agar diffusion assay, minimum inhibitory concentration (MIC), outer and inner cell membrane permeability.
33100848	3	44	theme	minimum	501:507	arg1	assay					494:498	agar diffusion assay	479:498	agar diffusion assay	479:498	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides were examined by means of agar diffusion assay, minimum inhibitory concentration (MIC), outer and inner cell membrane permeability.
33100848	3	44	theme	minimum	501:507	arg1	MIC					535:537	MIC	535:537	MIC	535:537	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides were examined by means of agar diffusion assay, minimum inhibitory concentration (MIC), outer and inner cell membrane permeability.
33100848	2	45	theme	present	212:218	arg1	investigation					220:232	The present investigation	208:232	The present investigation	208:232	The present investigation aimed to examine the antibacterial, anti-scavenging and cytotoxic potential of garden cress (GC) polysaccharides.
33100848	0	46	theme	garden	58:63	arg1	cress					65:69	garden cress	58:69	garden cress	58:69	Anti-bacterial, anti-scavenging and cytotoxic activity of garden cress polysaccharides.
33100848	7	47	theme	reactive	1219:1226	arg1	ROS					1244:1246	ROS	1244:1246	ROS	1244:1246	Compared to the standard inhibitor, the GC polysaccharides showed essential inhibitor assays in a very dose dependent approach, and notable actions to scavenge reactive oxygen species (ROS) are also due to the large quantities of hydrophilic polyphenols.
33100848	7	47	theme	reactive	1219:1226	arg1	species					1235:1241	reactive oxygen species	1219:1241	reactive oxygen species (ROS)	1219:1247	Compared to the standard inhibitor, the GC polysaccharides showed essential inhibitor assays in a very dose dependent approach, and notable actions to scavenge reactive oxygen species (ROS) are also due to the large quantities of hydrophilic polyphenols.
33100848	7	48	theme	inhibitor	1135:1143	arg1	assays					1145:1150	essential inhibitor assays	1125:1150	essential inhibitor assays	1125:1150	Compared to the standard inhibitor, the GC polysaccharides showed essential inhibitor assays in a very dose dependent approach, and notable actions to scavenge reactive oxygen species (ROS) are also due to the large quantities of hydrophilic polyphenols.
33100848	11	49	theme	cell	1862:1865	arg1	lines					1867:1871	different carcinoma cell lines	1842:1871	different carcinoma cell lines	1842:1871	The findings obtained in this study indicate that GC polysaccharides has antibacterial and has a possible source of natural antioxidant and also has cytotoxic effect on different carcinoma cell lines.
33100848	10	50	theme	GC	1536:1537	arg1	polysaccharides					1539:1553	GC polysaccharides	1536:1553	GC polysaccharides at a dose of 500 µg ml-1	1536:1578	GC polysaccharides at a dose of 500 µg ml-1 exhibited higher anti-tumor activity in both HeLa (65.33 ± 3.75%) and HepG2 (60.33 ± 3.48%).
33100848	11	51	contain	has	1764:1766	arg2	source					1779:1784	a possible source	1768:1784	a possible source of natural antioxidant	1768:1807	The findings obtained in this study indicate that GC polysaccharides has antibacterial and has a possible source of natural antioxidant and also has cytotoxic effect on different carcinoma cell lines.
33100848	11	51	contain	has	1764:1766	arg1	polysaccharides					1726:1740	GC polysaccharides	1723:1740	GC polysaccharides	1723:1740	The findings obtained in this study indicate that GC polysaccharides has antibacterial and has a possible source of natural antioxidant and also has cytotoxic effect on different carcinoma cell lines.
33100848	10	52	theme	higher	1590:1595	arg1	activity					1608:1615	higher anti-tumor activity	1590:1615	higher anti-tumor activity	1590:1615	GC polysaccharides at a dose of 500 µg ml-1 exhibited higher anti-tumor activity in both HeLa (65.33 ± 3.75%) and HepG2 (60.33 ± 3.48%).
33100848	3	53	theme	cell	557:560	arg1	assay					494:498	agar diffusion assay	479:498	agar diffusion assay	479:498	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides were examined by means of agar diffusion assay, minimum inhibitory concentration (MIC), outer and inner cell membrane permeability.
33100848	3	53	theme	cell	557:560	arg1	permeability					571:582	outer and inner cell membrane permeability	541:582	outer and inner cell membrane permeability	541:582	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides were examined by means of agar diffusion assay, minimum inhibitory concentration (MIC), outer and inner cell membrane permeability.
33100848	9	54	theme	GC	1423:1424	arg1	polysaccharides					1426:1440	The GC polysaccharides	1419:1440	The GC polysaccharides	1419:1440	The GC polysaccharides diminish the cell viability percentage of HeLa and HepG2 in a concentration dependent manner.
33100848	5	55	theme	HepG2	920:924	arg1	lines					932:936	human cervical (HeLa) and liver carcinoma (HepG2) cell lines	877:936	lines	932:936	Cytotoxicity potential of GC polysaccharides were evaluated by MTT assay in human cervical (HeLa) and liver carcinoma (HepG2) cell lines.
33100848	4	56	theme	GC	614:615	arg1	polysaccharides					617:631	the GC polysaccharides	610:631	the GC polysaccharides	610:631	Antioxidant potential of the GC polysaccharides were performed by free radical DPPH scavenging, superoxide anion scavenging, hydroxyl radical scavenging, reducing power potential assay, and hydrogen peroxide method.
33100848	10	57	theme	500 µg ml-1	1568:1578	arg1	dose					1560:1563	a dose	1558:1563	a dose of 500 µg ml-1	1558:1578	GC polysaccharides at a dose of 500 µg ml-1 exhibited higher anti-tumor activity in both HeLa (65.33 ± 3.75%) and HepG2 (60.33 ± 3.48%).
33100848	7	58	theme	GC	1099:1100	arg1	polysaccharides					1102:1116	the GC polysaccharides	1095:1116	the GC polysaccharides	1095:1116	Compared to the standard inhibitor, the GC polysaccharides showed essential inhibitor assays in a very dose dependent approach, and notable actions to scavenge reactive oxygen species (ROS) are also due to the large quantities of hydrophilic polyphenols.
33100848	2	59	theme	garden	313:318	arg1	cress					320:324	garden cress	313:324	garden cress (GC) polysaccharides	313:345	The present investigation aimed to examine the antibacterial, anti-scavenging and cytotoxic potential of garden cress (GC) polysaccharides.
33100848	2	59	theme	garden	313:318	arg1	GC					327:328	GC	327:328	GC	327:328	The present investigation aimed to examine the antibacterial, anti-scavenging and cytotoxic potential of garden cress (GC) polysaccharides.
33100848	1	60	theme	biological	185:194	arg1	activities					196:205	varying pharmacological and biological activities	157:205	varying pharmacological and biological activities	157:205	Plants polysaccharides are an infinite stock of drug composites with varying pharmacological and biological activities.
33100848	4	61	theme	superoxide	681:690	arg1	anion					692:696	superoxide anion	681:696	superoxide anion scavenging	681:707	Antioxidant potential of the GC polysaccharides were performed by free radical DPPH scavenging, superoxide anion scavenging, hydroxyl radical scavenging, reducing power potential assay, and hydrogen peroxide method.
33100848	9	62	theme	cell	1455:1458	arg1	percentage					1470:1479	the cell viability percentage	1451:1479	the cell viability percentage of HeLa and HepG2	1451:1497	The GC polysaccharides diminish the cell viability percentage of HeLa and HepG2 in a concentration dependent manner.
33100848	11	63	theme	antioxidant	1797:1807	arg1	source					1779:1784	a possible source	1768:1784	a possible source of natural antioxidant	1768:1807	The findings obtained in this study indicate that GC polysaccharides has antibacterial and has a possible source of natural antioxidant and also has cytotoxic effect on different carcinoma cell lines.
33100848	7	64	theme	standard	1075:1082	arg1	inhibitor					1084:1092	the standard inhibitor	1071:1092	the standard inhibitor	1071:1092	Compared to the standard inhibitor, the GC polysaccharides showed essential inhibitor assays in a very dose dependent approach, and notable actions to scavenge reactive oxygen species (ROS) are also due to the large quantities of hydrophilic polyphenols.
33100848	4	65	theme	radical	656:662	arg1	scavenging					669:678	free radical DPPH scavenging	651:678	free radical DPPH scavenging	651:678	Antioxidant potential of the GC polysaccharides were performed by free radical DPPH scavenging, superoxide anion scavenging, hydroxyl radical scavenging, reducing power potential assay, and hydrogen peroxide method.
33100848	9	66	theme	viability	1460:1468	arg1	percentage					1470:1479	the cell viability percentage	1451:1479	the cell viability percentage of HeLa and HepG2	1451:1497	The GC polysaccharides diminish the cell viability percentage of HeLa and HepG2 in a concentration dependent manner.
33100848	5	67	theme	cervical	883:890	arg1	HeLa					893:896	human cervical (HeLa) and liver carcinoma (HepG2) cell lines	877:936	HeLa	893:896	Cytotoxicity potential of GC polysaccharides were evaluated by MTT assay in human cervical (HeLa) and liver carcinoma (HepG2) cell lines.
33100848	6	68	theme	GC	964:965	arg1	MIC					983:985	GC polysaccharides MIC	964:985	GC polysaccharides MIC	964:985	The findings showed that GC polysaccharides MIC were 1.06 and 0.56 mg mL-1 against E. coli and S. aureus, respectively.
33100848	11	69	theme	cytotoxic	1822:1830	arg1	effect					1832:1837	cytotoxic effect	1822:1837	cytotoxic effect on different carcinoma cell lines	1822:1871	The findings obtained in this study indicate that GC polysaccharides has antibacterial and has a possible source of natural antioxidant and also has cytotoxic effect on different carcinoma cell lines.
33100848	9	70	theme	HepG2	1493:1497	arg1	percentage					1470:1479	the cell viability percentage	1451:1479	the cell viability percentage of HeLa and HepG2	1451:1497	The GC polysaccharides diminish the cell viability percentage of HeLa and HepG2 in a concentration dependent manner.
33100848	4	71	theme	reducing	739:746	arg1	assay					764:768	reducing power potential assay	739:768	reducing power potential assay	739:768	Antioxidant potential of the GC polysaccharides were performed by free radical DPPH scavenging, superoxide anion scavenging, hydroxyl radical scavenging, reducing power potential assay, and hydrogen peroxide method.
33100848	7	72	theme	dependent	1167:1175	arg1	approach					1177:1184	a very dose dependent approach	1155:1184	a very dose dependent approach	1155:1184	Compared to the standard inhibitor, the GC polysaccharides showed essential inhibitor assays in a very dose dependent approach, and notable actions to scavenge reactive oxygen species (ROS) are also due to the large quantities of hydrophilic polyphenols.
33100848	1	73	theme	drug	136:139	arg1	composites					141:150	drug composites	136:150	drug composites	136:150	Plants polysaccharides are an infinite stock of drug composites with varying pharmacological and biological activities.
33100848	4	74	theme	potential	754:762	arg1	assay					764:768	reducing power potential assay	739:768	reducing power potential assay	739:768	Antioxidant potential of the GC polysaccharides were performed by free radical DPPH scavenging, superoxide anion scavenging, hydroxyl radical scavenging, reducing power potential assay, and hydrogen peroxide method.
33100848	9	75	theme	dependent	1518:1526	arg1	manner					1528:1533	a concentration dependent manner	1502:1533	a concentration dependent manner	1502:1533	The GC polysaccharides diminish the cell viability percentage of HeLa and HepG2 in a concentration dependent manner.
33100848	5	76	theme	carcinoma	909:917	arg1	lines					932:936	human cervical (HeLa) and liver carcinoma (HepG2) cell lines	877:936	lines	932:936	Cytotoxicity potential of GC polysaccharides were evaluated by MTT assay in human cervical (HeLa) and liver carcinoma (HepG2) cell lines.
33100848	4	77	theme	hydroxyl	710:717	arg1	scavenging					727:736	hydroxyl radical scavenging	710:736	hydroxyl radical scavenging	710:736	Antioxidant potential of the GC polysaccharides were performed by free radical DPPH scavenging, superoxide anion scavenging, hydroxyl radical scavenging, reducing power potential assay, and hydrogen peroxide method.
33100848	5	78	theme	Cytotoxicity	801:812	arg1	potential					814:822	Cytotoxicity potential	801:822	Cytotoxicity potential of GC polysaccharides	801:844	Cytotoxicity potential of GC polysaccharides were evaluated by MTT assay in human cervical (HeLa) and liver carcinoma (HepG2) cell lines.
33100848	1	79	theme	pharmacological	165:179	arg1	activities					196:205	varying pharmacological and biological activities	157:205	varying pharmacological and biological activities	157:205	Plants polysaccharides are an infinite stock of drug composites with varying pharmacological and biological activities.
33100848	3	80	theme	antibacterial	352:364	arg1	effects					366:372	The antibacterial effects	348:372	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides	348:451	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides were examined by means of agar diffusion assay, minimum inhibitory concentration (MIC), outer and inner cell membrane permeability.
33100848	8	81	theme	IC50	1318:1321	arg1	values					1323:1328	The IC50 values	1314:1328	The IC50 values of all tested parameters	1314:1353	The IC50 values of all tested parameters were measured against standard ascorbic acid antioxidant agent.
33100848	11	82	theme	possible	1770:1777	arg1	source					1779:1784	a possible source	1768:1784	a possible source of natural antioxidant	1768:1807	The findings obtained in this study indicate that GC polysaccharides has antibacterial and has a possible source of natural antioxidant and also has cytotoxic effect on different carcinoma cell lines.
33100848	3	83	theme	as	398:399	arg1	effects					366:372	The antibacterial effects	348:372	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides	348:451	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides were examined by means of agar diffusion assay, minimum inhibitory concentration (MIC), outer and inner cell membrane permeability.
33100848	7	84	theme	notable	1191:1197	arg1	actions					1199:1205	notable actions	1191:1205	notable actions to scavenge reactive oxygen species (ROS)	1191:1247	Compared to the standard inhibitor, the GC polysaccharides showed essential inhibitor assays in a very dose dependent approach, and notable actions to scavenge reactive oxygen species (ROS) are also due to the large quantities of hydrophilic polyphenols.
33100848	11	85	from	effect	1832:1837	arg1	lines					1867:1871	different carcinoma cell lines	1842:1871	different carcinoma cell lines	1842:1871	The findings obtained in this study indicate that GC polysaccharides has antibacterial and has a possible source of natural antioxidant and also has cytotoxic effect on different carcinoma cell lines.
33100848	8	86	theme	tested	1337:1342	arg1	parameters					1344:1353	all tested parameters	1333:1353	all tested parameters	1333:1353	The IC50 values of all tested parameters were measured against standard ascorbic acid antioxidant agent.
33100848	1	87	with	stock	127:131	arg1	activities					196:205	varying pharmacological and biological activities	157:205	varying pharmacological and biological activities	157:205	Plants polysaccharides are an infinite stock of drug composites with varying pharmacological and biological activities.
33100848	5	88	theme	MTT	864:866	arg1	assay					868:872	MTT assay	864:872	MTT assay	864:872	Cytotoxicity potential of GC polysaccharides were evaluated by MTT assay in human cervical (HeLa) and liver carcinoma (HepG2) cell lines.
33100848	7	89	theme	hydrophilic	1289:1299	arg1	polyphenols					1301:1311	hydrophilic polyphenols	1289:1311	hydrophilic polyphenols	1289:1311	Compared to the standard inhibitor, the GC polysaccharides showed essential inhibitor assays in a very dose dependent approach, and notable actions to scavenge reactive oxygen species (ROS) are also due to the large quantities of hydrophilic polyphenols.
33100848	4	90	theme	hydrogen	775:782	arg1	peroxide					784:791	hydrogen peroxide	775:791	hydrogen peroxide method	775:798	Antioxidant potential of the GC polysaccharides were performed by free radical DPPH scavenging, superoxide anion scavenging, hydroxyl radical scavenging, reducing power potential assay, and hydrogen peroxide method.
33100848	9	91	theme	HeLa	1484:1487	arg1	percentage					1470:1479	the cell viability percentage	1451:1479	the cell viability percentage of HeLa and HepG2	1451:1497	The GC polysaccharides diminish the cell viability percentage of HeLa and HepG2 in a concentration dependent manner.
33100848	8	92	theme	ascorbic	1386:1393	arg1	agent					1412:1416	standard ascorbic acid antioxidant agent	1377:1416	standard ascorbic acid antioxidant agent	1377:1416	The IC50 values of all tested parameters were measured against standard ascorbic acid antioxidant agent.
33100848	3	93	theme	diffusion	484:492	arg1	concentration					520:532	minimum inhibitory concentration	501:532	minimum inhibitory concentration (MIC)	501:538	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides were examined by means of agar diffusion assay, minimum inhibitory concentration (MIC), outer and inner cell membrane permeability.
33100848	3	93	theme	diffusion	484:492	arg1	assay					494:498	agar diffusion assay	479:498	agar diffusion assay	479:498	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides were examined by means of agar diffusion assay, minimum inhibitory concentration (MIC), outer and inner cell membrane permeability.
33100848	3	93	theme	diffusion	484:492	arg1	permeability					571:582	outer and inner cell membrane permeability	541:582	outer and inner cell membrane permeability	541:582	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides were examined by means of agar diffusion assay, minimum inhibitory concentration (MIC), outer and inner cell membrane permeability.
33100848	5	94	theme	polysaccharides	830:844	arg1	potential					814:822	Cytotoxicity potential	801:822	Cytotoxicity potential of GC polysaccharides	801:844	Cytotoxicity potential of GC polysaccharides were evaluated by MTT assay in human cervical (HeLa) and liver carcinoma (HepG2) cell lines.
33100848	3	95	theme	GC	434:435	arg1	polysaccharides					437:451	GC polysaccharides	434:451	GC polysaccharides	434:451	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides were examined by means of agar diffusion assay, minimum inhibitory concentration (MIC), outer and inner cell membrane permeability.
33100848	7	96	dep	actions	1199:1205	arg1	scavenge					1210:1217	scavenge	1210:1217	to scavenge reactive oxygen species (ROS)	1207:1247	Compared to the standard inhibitor, the GC polysaccharides showed essential inhibitor assays in a very dose dependent approach, and notable actions to scavenge reactive oxygen species (ROS) are also due to the large quantities of hydrophilic polyphenols.
33100848	8	97	theme	antioxidant	1400:1410	arg1	agent					1412:1416	standard ascorbic acid antioxidant agent	1377:1416	standard ascorbic acid antioxidant agent	1377:1416	The IC50 values of all tested parameters were measured against standard ascorbic acid antioxidant agent.
33100848	11	98	theme	carcinoma	1852:1860	arg1	lines					1867:1871	different carcinoma cell lines	1842:1871	different carcinoma cell lines	1842:1871	The findings obtained in this study indicate that GC polysaccharides has antibacterial and has a possible source of natural antioxidant and also has cytotoxic effect on different carcinoma cell lines.
33100848	11	99	contain	has	1818:1820	arg2	effect					1832:1837	cytotoxic effect	1822:1837	cytotoxic effect on different carcinoma cell lines	1822:1871	The findings obtained in this study indicate that GC polysaccharides has antibacterial and has a possible source of natural antioxidant and also has cytotoxic effect on different carcinoma cell lines.
33100848	11	99	contain	has	1818:1820	arg1	polysaccharides					1726:1740	GC polysaccharides	1723:1740	GC polysaccharides	1723:1740	The findings obtained in this study indicate that GC polysaccharides has antibacterial and has a possible source of natural antioxidant and also has cytotoxic effect on different carcinoma cell lines.
33100848	3	100	dep	as	398:399	arg1	aureus					424:429	aureus	424:429	aureus	424:429	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides were examined by means of agar diffusion assay, minimum inhibitory concentration (MIC), outer and inner cell membrane permeability.
33100848	3	101	theme	inhibitory	509:518	arg1	concentration					520:532	minimum inhibitory concentration	501:532	minimum inhibitory concentration (MIC)	501:538	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides were examined by means of agar diffusion assay, minimum inhibitory concentration (MIC), outer and inner cell membrane permeability.
33100848	3	101	theme	inhibitory	509:518	arg1	assay					494:498	agar diffusion assay	479:498	agar diffusion assay	479:498	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides were examined by means of agar diffusion assay, minimum inhibitory concentration (MIC), outer and inner cell membrane permeability.
33100848	3	101	theme	inhibitory	509:518	arg1	MIC					535:537	MIC	535:537	MIC	535:537	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides were examined by means of agar diffusion assay, minimum inhibitory concentration (MIC), outer and inner cell membrane permeability.
33100848	4	102	theme	free	651:654	arg1	scavenging					669:678	free radical DPPH scavenging	651:678	free radical DPPH scavenging	651:678	Antioxidant potential of the GC polysaccharides were performed by free radical DPPH scavenging, superoxide anion scavenging, hydroxyl radical scavenging, reducing power potential assay, and hydrogen peroxide method.
33100848	3	103	theme	outer	541:545	arg1	assay					494:498	agar diffusion assay	479:498	agar diffusion assay	479:498	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides were examined by means of agar diffusion assay, minimum inhibitory concentration (MIC), outer and inner cell membrane permeability.
33100848	3	103	theme	outer	541:545	arg1	permeability					571:582	outer and inner cell membrane permeability	541:582	outer and inner cell membrane permeability	541:582	The antibacterial effects vs Escherichia coli and as well as Staphylococcus aureus of GC polysaccharides were examined by means of agar diffusion assay, minimum inhibitory concentration (MIC), outer and inner cell membrane permeability.
34632015	2	0	theme	mustard	324:330	arg1	seeds					332:336	mustard seeds	324:336	mustard seeds	324:336	The 18 accessions of mustard seeds were selected to be representative of genetic, agronomical and technological variabilities.
34632015	0	1	theme	proteins	61:68	arg1	Data					0:3	Data	0:3	Data on agronomic traits	0:23	Data on agronomic traits, biochemical composition of lipids, proteins and polysaccharides and rheological measurement in a brown mustard seed collection.
34632015	0	1	theme	proteins	61:68	arg1	composition					38:48	biochemical composition	26:48	biochemical composition of lipids, proteins and polysaccharides	26:88	Data on agronomic traits, biochemical composition of lipids, proteins and polysaccharides and rheological measurement in a brown mustard seed collection.
34632015	0	1	theme	proteins	61:68	arg1	measurement					106:116	rheological measurement	94:116	rheological measurement in a brown mustard	94:135	Data on agronomic traits, biochemical composition of lipids, proteins and polysaccharides and rheological measurement in a brown mustard seed collection.
34632015	1	2	from	France	295:300	arg1	production					281:290	production	281:290	production in France	281:300	The data were collected from a brown mustard seeds collection of 18 accessions during two years and in three distinct sites of production in France.
34632015	1	2	from	France	295:300	arg1	sites					272:276	three distinct sites	257:276	three distinct sites of production in France	257:300	The data were collected from a brown mustard seeds collection of 18 accessions during two years and in three distinct sites of production in France.
34632015	4	3	theme	fine	634:637	arg1	composition					639:649	fine composition	634:649	fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides)	634:807	This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides).
34632015	0	4	from	Data	0:3	arg1	mustard					129:135	a brown mustard	121:135	a brown mustard	121:135	Data on agronomic traits, biochemical composition of lipids, proteins and polysaccharides and rheological measurement in a brown mustard seed collection.
34632015	0	4	from	Data	0:3	arg1	traits					18:23	agronomic traits	8:23	agronomic traits	8:23	Data on agronomic traits, biochemical composition of lipids, proteins and polysaccharides and rheological measurement in a brown mustard seed collection.
34632015	0	5	theme	polysaccharides	74:88	arg1	Data					0:3	Data	0:3	Data on agronomic traits	0:23	Data on agronomic traits, biochemical composition of lipids, proteins and polysaccharides and rheological measurement in a brown mustard seed collection.
34632015	0	5	theme	polysaccharides	74:88	arg1	composition					38:48	biochemical composition	26:48	biochemical composition of lipids, proteins and polysaccharides	26:88	Data on agronomic traits, biochemical composition of lipids, proteins and polysaccharides and rheological measurement in a brown mustard seed collection.
34632015	0	5	theme	polysaccharides	74:88	arg1	measurement					106:116	rheological measurement	94:116	rheological measurement in a brown mustard	94:135	Data on agronomic traits, biochemical composition of lipids, proteins and polysaccharides and rheological measurement in a brown mustard seed collection.
34632015	1	6	from	production	281:290	arg1	France					295:300	France	295:300	France	295:300	The data were collected from a brown mustard seeds collection of 18 accessions during two years and in three distinct sites of production in France.
34632015	3	7	theme	"	476:476	arg1	area					478:481	the "Bourgogne" area	462:481	the "Bourgogne" area	462:481	All accessions were produced in the "Bourgogne" area.
34632015	4	8	theme	genotyping	538:547	arg1	data					549:552	genotyping data	538:552	genotyping data	538:552	This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides).
34632015	0	9	from	composition	38:48	arg1	mustard					129:135	a brown mustard	121:135	a brown mustard	121:135	Data on agronomic traits, biochemical composition of lipids, proteins and polysaccharides and rheological measurement in a brown mustard seed collection.
34632015	0	9	from	composition	38:48	arg1	traits					18:23	agronomic traits	8:23	agronomic traits	8:23	Data on agronomic traits, biochemical composition of lipids, proteins and polysaccharides and rheological measurement in a brown mustard seed collection.
34632015	4	10	theme	mustard	577:583	arg1	seeds					585:589	mustard seeds	577:589	mustard seeds (lipids, proteins and polysaccharides)	577:628	This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides).
34632015	4	11	theme	fatty	736:740	arg1	acids					742:746	fatty acids	736:746	fatty acids	736:746	This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides).
34632015	0	12	theme	rheological	94:104	arg1	measurement					106:116	rheological measurement	94:116	rheological measurement in a brown mustard	94:135	Data on agronomic traits, biochemical composition of lipids, proteins and polysaccharides and rheological measurement in a brown mustard seed collection.
34632015	1	13	from	sites	272:276	arg1	France					295:300	France	295:300	France	295:300	The data were collected from a brown mustard seeds collection of 18 accessions during two years and in three distinct sites of production in France.
34632015	6	14	theme	end-uses	1102:1109	arg1	properties					1111:1120	the end-uses properties	1098:1120	the end-uses properties (paste mustard quality)	1098:1144	These data can be reused by food industries, breeders and geneticists in order to understand pedoclimatic effects (year and location) and the relation between mustard seed composition and the end-uses properties (paste mustard quality).
34632015	6	14	theme	end-uses	1102:1109	arg1	quality					1137:1143	paste mustard quality	1123:1143	paste mustard quality	1123:1143	These data can be reused by food industries, breeders and geneticists in order to understand pedoclimatic effects (year and location) and the relation between mustard seed composition and the end-uses properties (paste mustard quality).
34632015	6	15	theme	paste	1123:1127	arg1	properties					1111:1120	the end-uses properties	1098:1120	the end-uses properties (paste mustard quality)	1098:1144	These data can be reused by food industries, breeders and geneticists in order to understand pedoclimatic effects (year and location) and the relation between mustard seed composition and the end-uses properties (paste mustard quality).
34632015	6	15	theme	paste	1123:1127	arg1	quality					1137:1143	paste mustard quality	1123:1143	paste mustard quality	1123:1143	These data can be reused by food industries, breeders and geneticists in order to understand pedoclimatic effects (year and location) and the relation between mustard seed composition and the end-uses properties (paste mustard quality).
34632015	2	16	theme	variabilities	415:427	arg1	representative					358:371	representative	358:371	representative	358:371	The 18 accessions of mustard seeds were selected to be representative of genetic, agronomical and technological variabilities.
34632015	2	16	theme	variabilities	415:427	arg1	accessions					310:319	The 18 accessions	303:319	The 18 accessions of mustard seeds	303:336	The 18 accessions of mustard seeds were selected to be representative of genetic, agronomical and technological variabilities.
34632015	6	17	theme	food	938:941	arg1	industries					943:952	food industries	938:952	food industries	938:952	These data can be reused by food industries, breeders and geneticists in order to understand pedoclimatic effects (year and location) and the relation between mustard seed composition and the end-uses properties (paste mustard quality).
34632015	5	18	theme	potential	840:848	arg1	property					862:869	the potential rheological property	836:869	the potential rheological property of each accessions	836:888	Additional data regarding the potential rheological property of each accessions were also reported.
34632015	2	19	theme	technological	401:413	arg1	variabilities					415:427	genetic, agronomical and technological variabilities	376:427	genetic, agronomical and technological variabilities	376:427	The 18 accessions of mustard seeds were selected to be representative of genetic, agronomical and technological variabilities.
34632015	6	20	theme	pedoclimatic	1003:1014	arg1	effects					1016:1022	pedoclimatic effects	1003:1022	pedoclimatic effects (year and location)	1003:1042	These data can be reused by food industries, breeders and geneticists in order to understand pedoclimatic effects (year and location) and the relation between mustard seed composition and the end-uses properties (paste mustard quality).
34632015	6	20	theme	pedoclimatic	1003:1014	arg1	location					1034:1041	location	1034:1041	location	1034:1041	These data can be reused by food industries, breeders and geneticists in order to understand pedoclimatic effects (year and location) and the relation between mustard seed composition and the end-uses properties (paste mustard quality).
34632015	6	20	theme	pedoclimatic	1003:1014	arg1	year					1025:1028	year	1025:1028	year	1025:1028	These data can be reused by food industries, breeders and geneticists in order to understand pedoclimatic effects (year and location) and the relation between mustard seed composition and the end-uses properties (paste mustard quality).
34632015	2	21	theme	agronomical	385:395	arg1	variabilities					415:427	genetic, agronomical and technological variabilities	376:427	genetic, agronomical and technological variabilities	376:427	The 18 accessions of mustard seeds were selected to be representative of genetic, agronomical and technological variabilities.
34632015	0	22	theme	agronomic	8:16	arg1	traits					18:23	agronomic traits	8:23	agronomic traits	8:23	Data on agronomic traits, biochemical composition of lipids, proteins and polysaccharides and rheological measurement in a brown mustard seed collection.
34632015	1	23	theme	distinct	263:270	arg1	production					281:290	production	281:290	production in France	281:300	The data were collected from a brown mustard seeds collection of 18 accessions during two years and in three distinct sites of production in France.
34632015	1	23	theme	distinct	263:270	arg1	sites					272:276	three distinct sites	257:276	three distinct sites of production in France	257:300	The data were collected from a brown mustard seeds collection of 18 accessions during two years and in three distinct sites of production in France.
34632015	6	24	theme	mustard	1129:1135	arg1	properties					1111:1120	the end-uses properties	1098:1120	the end-uses properties (paste mustard quality)	1098:1144	These data can be reused by food industries, breeders and geneticists in order to understand pedoclimatic effects (year and location) and the relation between mustard seed composition and the end-uses properties (paste mustard quality).
34632015	6	24	theme	mustard	1129:1135	arg1	quality					1137:1143	paste mustard quality	1123:1143	paste mustard quality	1123:1143	These data can be reused by food industries, breeders and geneticists in order to understand pedoclimatic effects (year and location) and the relation between mustard seed composition and the end-uses properties (paste mustard quality).
34632015	4	25	dep	properties	724:733	arg1	acids					742:746	fatty acids	736:746	fatty acids	736:746	This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides).
34632015	4	25	dep	properties	724:733	arg1	proteins					757:764	storage proteins	749:764	storage proteins	749:764	This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides).
34632015	4	25	dep	properties	724:733	arg1	composition					777:787	osidic composition	770:787	osidic composition of polysaccharides	770:806	This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides).
34632015	5	26	theme	each	874:877	arg1	accessions					879:888	each accessions	874:888	each accessions	874:888	Additional data regarding the potential rheological property of each accessions were also reported.
34632015	1	27	theme	brown	185:189	arg1	collection					205:214	a brown mustard seeds collection	183:214	a brown mustard seeds collection of 18 accessions	183:231	The data were collected from a brown mustard seeds collection of 18 accessions during two years and in three distinct sites of production in France.
34632015	0	28	theme	biochemical	26:36	arg1	composition					38:48	biochemical composition	26:48	biochemical composition of lipids, proteins and polysaccharides	26:88	Data on agronomic traits, biochemical composition of lipids, proteins and polysaccharides and rheological measurement in a brown mustard seed collection.
34632015	2	29	theme	genetic	376:382	arg1	variabilities					415:427	genetic, agronomical and technological variabilities	376:427	genetic, agronomical and technological variabilities	376:427	The 18 accessions of mustard seeds were selected to be representative of genetic, agronomical and technological variabilities.
34632015	4	30	theme	osidic	770:775	arg1	composition					777:787	osidic composition	770:787	osidic composition of polysaccharides	770:806	This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides).
34632015	1	31	theme	mustard	191:197	arg1	collection					205:214	a brown mustard seeds collection	183:214	a brown mustard seeds collection of 18 accessions	183:231	The data were collected from a brown mustard seeds collection of 18 accessions during two years and in three distinct sites of production in France.
34632015	0	32	theme	brown	123:127	arg1	mustard					129:135	a brown mustard	121:135	a brown mustard	121:135	Data on agronomic traits, biochemical composition of lipids, proteins and polysaccharides and rheological measurement in a brown mustard seed collection.
34632015	4	33	theme	technological	710:722	arg1	properties					724:733	the technological properties	706:733	the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides)	706:807	This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides).
34632015	1	34	theme	seeds	199:203	arg1	collection					205:214	a brown mustard seeds collection	183:214	a brown mustard seeds collection of 18 accessions	183:231	The data were collected from a brown mustard seeds collection of 18 accessions during two years and in three distinct sites of production in France.
34632015	1	35	theme	production	281:290	arg1	production					281:290	production	281:290	production in France	281:300	The data were collected from a brown mustard seeds collection of 18 accessions during two years and in three distinct sites of production in France.
34632015	1	35	theme	production	281:290	arg1	sites					272:276	three distinct sites	257:276	three distinct sites of production in France	257:300	The data were collected from a brown mustard seeds collection of 18 accessions during two years and in three distinct sites of production in France.
34632015	4	36	dep	seeds	585:589	arg1	proteins					600:607	proteins	600:607	proteins	600:607	This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides).
34632015	4	36	dep	seeds	585:589	arg1	lipids					592:597	lipids	592:597	lipids	592:597	This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides).
34632015	4	36	dep	seeds	585:589	arg1	polysaccharides					613:627	polysaccharides	613:627	polysaccharides	613:627	This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides).
34632015	5	37	theme	accessions	879:888	arg1	property					862:869	the potential rheological property	836:869	the potential rheological property of each accessions	836:888	Additional data regarding the potential rheological property of each accessions were also reported.
34632015	0	38	from	measurement	106:116	arg1	mustard					129:135	a brown mustard	121:135	a brown mustard	121:135	Data on agronomic traits, biochemical composition of lipids, proteins and polysaccharides and rheological measurement in a brown mustard seed collection.
34632015	0	38	from	measurement	106:116	arg1	traits					18:23	agronomic traits	8:23	agronomic traits	8:23	Data on agronomic traits, biochemical composition of lipids, proteins and polysaccharides and rheological measurement in a brown mustard seed collection.
34632015	4	39	theme	polysaccharides	792:806	arg1	acids					742:746	fatty acids	736:746	fatty acids	736:746	This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides).
34632015	4	39	theme	polysaccharides	792:806	arg1	proteins					757:764	storage proteins	749:764	storage proteins	749:764	This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides).
34632015	4	39	theme	polysaccharides	792:806	arg1	composition					777:787	osidic composition	770:787	osidic composition of polysaccharides	770:806	This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides).
34632015	3	40	theme	Bourgogne	467:475	arg1	area					478:481	the "Bourgogne" area	462:481	the "Bourgogne" area	462:481	All accessions were produced in the "Bourgogne" area.
34632015	4	41	theme	agronomical	507:517	arg1	data					519:522	agronomical data	507:522	agronomical data (PMG, yield)	507:535	This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides).
34632015	4	42	theme	macronutrients	667:680	arg1	data					549:552	genotyping data	538:552	genotyping data	538:552	This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides).
34632015	4	42	theme	macronutrients	667:680	arg1	data					519:522	agronomical data	507:522	agronomical data (PMG, yield)	507:535	This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides).
34632015	4	42	theme	macronutrients	667:680	arg1	composition					639:649	fine composition	634:649	fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides)	634:807	This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides).
34632015	4	42	theme	macronutrients	667:680	arg1	composition					562:572	global composition	555:572	global composition of mustard seeds (lipids, proteins and polysaccharides)	555:628	This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides).
34632015	6	43	theme	mustard	1069:1075	arg1	composition					1082:1092	mustard seed composition	1069:1092	mustard seed composition	1069:1092	These data can be reused by food industries, breeders and geneticists in order to understand pedoclimatic effects (year and location) and the relation between mustard seed composition and the end-uses properties (paste mustard quality).
34632015	4	44	theme	global	555:560	arg1	composition					562:572	global composition	555:572	global composition of mustard seeds (lipids, proteins and polysaccharides)	555:628	This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides).
34632015	6	45	dep	effects	1016:1022	arg1	effects					1016:1022	pedoclimatic effects	1003:1022	pedoclimatic effects (year and location)	1003:1042	These data can be reused by food industries, breeders and geneticists in order to understand pedoclimatic effects (year and location) and the relation between mustard seed composition and the end-uses properties (paste mustard quality).
34632015	6	45	dep	effects	1016:1022	arg1	location					1034:1041	location	1034:1041	location	1034:1041	These data can be reused by food industries, breeders and geneticists in order to understand pedoclimatic effects (year and location) and the relation between mustard seed composition and the end-uses properties (paste mustard quality).
34632015	6	45	dep	effects	1016:1022	arg1	year					1025:1028	year	1025:1028	year	1025:1028	These data can be reused by food industries, breeders and geneticists in order to understand pedoclimatic effects (year and location) and the relation between mustard seed composition and the end-uses properties (paste mustard quality).
34632015	4	46	theme	previous	658:665	arg1	macronutrients					667:680	the previous macronutrients	654:680	the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides)	654:807	This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides).
34632015	6	47	theme	seed	1077:1080	arg1	composition					1082:1092	mustard seed composition	1069:1092	mustard seed composition	1069:1092	These data can be reused by food industries, breeders and geneticists in order to understand pedoclimatic effects (year and location) and the relation between mustard seed composition and the end-uses properties (paste mustard quality).
34632015	5	48	theme	Additional	810:819	arg1	data					821:824	Additional data	810:824	Additional data regarding the potential rheological property of each accessions	810:888	Additional data regarding the potential rheological property of each accessions were also reported.
34632015	4	49	theme	seeds	585:589	arg1	data					549:552	genotyping data	538:552	genotyping data	538:552	This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides).
34632015	4	49	theme	seeds	585:589	arg1	data					519:522	agronomical data	507:522	agronomical data (PMG, yield)	507:535	This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides).
34632015	4	49	theme	seeds	585:589	arg1	composition					639:649	fine composition	634:649	fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides)	634:807	This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides).
34632015	4	49	theme	seeds	585:589	arg1	composition					562:572	global composition	555:572	global composition of mustard seeds (lipids, proteins and polysaccharides)	555:628	This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides).
34632015	5	50	theme	rheological	850:860	arg1	property					862:869	the potential rheological property	836:869	the potential rheological property of each accessions	836:888	Additional data regarding the potential rheological property of each accessions were also reported.
34632015	0	51	theme	lipids	53:58	arg1	Data					0:3	Data	0:3	Data on agronomic traits	0:23	Data on agronomic traits, biochemical composition of lipids, proteins and polysaccharides and rheological measurement in a brown mustard seed collection.
34632015	0	51	theme	lipids	53:58	arg1	composition					38:48	biochemical composition	26:48	biochemical composition of lipids, proteins and polysaccharides	26:88	Data on agronomic traits, biochemical composition of lipids, proteins and polysaccharides and rheological measurement in a brown mustard seed collection.
34632015	0	51	theme	lipids	53:58	arg1	measurement					106:116	rheological measurement	94:116	rheological measurement in a brown mustard	94:135	Data on agronomic traits, biochemical composition of lipids, proteins and polysaccharides and rheological measurement in a brown mustard seed collection.
34632015	4	52	dep	data	519:522	arg1	PMG					525:527	PMG	525:527	PMG	525:527	This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides).
34632015	4	52	dep	data	519:522	arg1	yield					530:534	yield	530:534	yield	530:534	This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides).
34632015	4	53	theme	storage	749:755	arg1	proteins					757:764	storage proteins	749:764	storage proteins	749:764	This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides).
34632015	2	54	theme	seeds	332:336	arg1	accessions					310:319	The 18 accessions	303:319	The 18 accessions of mustard seeds	303:336	The 18 accessions of mustard seeds were selected to be representative of genetic, agronomical and technological variabilities.
34632015	2	54	theme	seeds	332:336	arg1	representative					358:371	representative	358:371	representative	358:371	The 18 accessions of mustard seeds were selected to be representative of genetic, agronomical and technological variabilities.
34632015	1	55	theme	accessions	222:231	arg1	collection					205:214	a brown mustard seeds collection	183:214	a brown mustard seeds collection of 18 accessions	183:231	The data were collected from a brown mustard seeds collection of 18 accessions during two years and in three distinct sites of production in France.
33999794	6	0	theme	amino	890:894	arg1	identity					901:908	The average amino acid identity	878:908	The average amino acid identity values (56.1-61.5 %) between ACPA22T and other Glycomycetaceae members with available genomes	878:1002	The average amino acid identity values (56.1-61.5 %) between ACPA22T and other Glycomycetaceae members with available genomes did not exceed the threshold reported for different genera.
33999794	6	0	theme	amino	890:894	arg1	%					928:928	56.1-61.5 %	918:928	56.1-61.5 %	918:928	The average amino acid identity values (56.1-61.5 %) between ACPA22T and other Glycomycetaceae members with available genomes did not exceed the threshold reported for different genera.
33999794	14	1	theme	Natronoglycomyces	2004:2020	arg1	genus					1985:1989	a new genus	1979:1989	a new genus	1979:1989	Based on the data obtained in this study, a new genus and species, Natronoglycomyces albus gen. nov., sp.
33999794	14	1	theme	Natronoglycomyces	2004:2020	arg1	sp					2039:2040	sp	2039:2040	sp	2039:2040	Based on the data obtained in this study, a new genus and species, Natronoglycomyces albus gen. nov., sp.
33999794	14	1	theme	Natronoglycomyces	2004:2020	arg1	nov.					2033:2036	Natronoglycomyces albus gen. nov.	2004:2036	Natronoglycomyces albus gen. nov.	2004:2036	Based on the data obtained in this study, a new genus and species, Natronoglycomyces albus gen. nov., sp.
33999794	7	2	theme	peptidoglycan	1200:1212	arg1	A1γ					1219:1221	the peptidoglycan type A1γ'	1196:1222	the peptidoglycan type A1γ'	1196:1222	The cell wall of ACPA22T contained meso-diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio, characteristic of the peptidoglycan type A1γ'.
33999794	10	3	theme	phosphatidylinositol	1504:1523	arg1	mannosides					1525:1534	phosphatidylinositol mannosides	1504:1534	phosphatidylinositol mannosides	1504:1534	The identified polar lipids were represented by phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides.
33999794	5	4	theme	gene-based	617:626	arg1	analysis					641:648	The 16S rRNA gene-based phylogenetic analysis	604:648	The 16S rRNA gene-based phylogenetic analysis	604:648	The 16S rRNA gene-based phylogenetic analysis demonstrated that strain ACPA22T formed a deep-branching lineage within the family Glycomycetaceae, with the highest sequence similarity value to Glycomyces buryatensis 18T (92.1 %) and Salininema proteolyticum Miq-4T (91.8 %).
33999794	4	5	theme	multiple	553:560	arg1	genes					583:587	multiple glycosidase-encoding genes	553:587	multiple glycosidase-encoding genes	553:587	The hydrolytic nature of ACPA22T was confirmed by two different growth-dependent methods and by the presence of multiple glycosidase-encoding genes in the genome.
33999794	2	6	theme	strain	154:159	arg1	actinobacterium					137:151	A haloalkaliphilic hydrolytic actinobacterium	107:151	A haloalkaliphilic hydrolytic actinobacterium	107:151	A haloalkaliphilic hydrolytic actinobacterium, strain ACPA22T, was enriched and isolated in pure culture from saline alkaline soil (soda solonchak) in northeastern Mongolia.
33999794	2	6	theme	strain	154:159	arg1	ACPA22T					161:167	strain ACPA22T	154:167	strain ACPA22T	154:167	A haloalkaliphilic hydrolytic actinobacterium, strain ACPA22T, was enriched and isolated in pure culture from saline alkaline soil (soda solonchak) in northeastern Mongolia.
33999794	7	7	theme	ACPA22T	1081:1087	arg1	wall					1073:1076	The cell wall	1064:1076	The cell wall of ACPA22T	1064:1087	The cell wall of ACPA22T contained meso-diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio, characteristic of the peptidoglycan type A1γ'.
33999794	5	8	theme	proteolyticum	847:859	arg1	Miq-4T					861:866	Salininema proteolyticum Miq-4T	836:866	Salininema proteolyticum Miq-4T (91.8 %)	836:875	The 16S rRNA gene-based phylogenetic analysis demonstrated that strain ACPA22T formed a deep-branching lineage within the family Glycomycetaceae, with the highest sequence similarity value to Glycomyces buryatensis 18T (92.1 %) and Salininema proteolyticum Miq-4T (91.8 %).
33999794	5	8	theme	proteolyticum	847:859	arg1	%					874:874	91.8 %	869:874	91.8 %	869:874	The 16S rRNA gene-based phylogenetic analysis demonstrated that strain ACPA22T formed a deep-branching lineage within the family Glycomycetaceae, with the highest sequence similarity value to Glycomyces buryatensis 18T (92.1 %) and Salininema proteolyticum Miq-4T (91.8 %).
33999794	12	9	theme	fatty	1743:1747	arg1	acids					1749:1753	The polar lipid fatty acids	1727:1753	The polar lipid fatty acids	1727:1753	The polar lipid fatty acids were dominated by anteiso-C17 : 0 and iso-C16 : 0.
33999794	5	10	theme	16S	608:610	arg1	rRNA					612:615	The 16S rRNA	604:615	The 16S rRNA gene-based phylogenetic analysis	604:648	The 16S rRNA gene-based phylogenetic analysis demonstrated that strain ACPA22T formed a deep-branching lineage within the family Glycomycetaceae, with the highest sequence similarity value to Glycomyces buryatensis 18T (92.1 %) and Salininema proteolyticum Miq-4T (91.8 %).
33999794	7	11	theme	meso-diaminopimelic	1099:1117	arg1	acid					1119:1122	meso-diaminopimelic acid	1099:1122	meso-diaminopimelic acid	1099:1122	The cell wall of ACPA22T contained meso-diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio, characteristic of the peptidoglycan type A1γ'.
33999794	15	12	theme	type	2069:2072	arg1	ACPA22T					2081:2087	the type strain ACPA22T	2065:2087	the type strain ACPA22T (=DSM 106290T=VKM Ac-2771T)	2065:2115	nov, is proposed with the type strain ACPA22T (=DSM 106290T=VKM Ac-2771T).
33999794	15	12	theme	type	2069:2072	arg1	Ac-2771T					2107:2114	=DSM 106290T=VKM Ac-2771T	2090:2114	=DSM 106290T=VKM Ac-2771T	2090:2114	nov, is proposed with the type strain ACPA22T (=DSM 106290T=VKM Ac-2771T).
33999794	6	13	dep	identity	901:908	arg1	values					910:915	values	910:915	The average amino acid identity values (56.1-61.5 %) between ACPA22T and other Glycomycetaceae members with available genomes	878:1002	The average amino acid identity values (56.1-61.5 %) between ACPA22T and other Glycomycetaceae members with available genomes did not exceed the threshold reported for different genera.
33999794	2	14	theme	saline	217:222	arg1	soil					233:236	saline alkaline soil	217:236	saline alkaline soil (soda solonchak) in northeastern Mongolia	217:278	A haloalkaliphilic hydrolytic actinobacterium, strain ACPA22T, was enriched and isolated in pure culture from saline alkaline soil (soda solonchak) in northeastern Mongolia.
33999794	2	14	theme	saline	217:222	arg1	solonchak					244:252	soda solonchak	239:252	soda solonchak	239:252	A haloalkaliphilic hydrolytic actinobacterium, strain ACPA22T, was enriched and isolated in pure culture from saline alkaline soil (soda solonchak) in northeastern Mongolia.
33999794	11	15	contain	had	1561:1563	arg2	lipids					1605:1610	a few unidentified characteristic polar lipids	1565:1610	a few unidentified characteristic polar lipids	1565:1610	In addition, the strain had a few unidentified characteristic polar lipids, including an amine-containing phospholipid with chromatographic mobility similar to that of phosphatidylinositol.
33999794	11	15	contain	had	1561:1563	arg2	phospholipid					1643:1654	an amine-containing phospholipid	1623:1654	an amine-containing phospholipid with chromatographic mobility similar to that of phosphatidylinositol	1623:1724	In addition, the strain had a few unidentified characteristic polar lipids, including an amine-containing phospholipid with chromatographic mobility similar to that of phosphatidylinositol.
33999794	11	15	contain	had	1561:1563	arg1	strain					1554:1559	the strain	1550:1559	the strain	1550:1559	In addition, the strain had a few unidentified characteristic polar lipids, including an amine-containing phospholipid with chromatographic mobility similar to that of phosphatidylinositol.
33999794	4	16	attach	presence	541:548	arg2	genes					583:587	multiple glycosidase-encoding genes	553:587	multiple glycosidase-encoding genes	553:587	The hydrolytic nature of ACPA22T was confirmed by two different growth-dependent methods and by the presence of multiple glycosidase-encoding genes in the genome.
33999794	4	16	attach	presence	541:548	arg1	genome					596:601	the genome	592:601	the genome	592:601	The hydrolytic nature of ACPA22T was confirmed by two different growth-dependent methods and by the presence of multiple glycosidase-encoding genes in the genome.
33999794	12	17	theme	lipid	1737:1741	arg1	acids					1749:1753	The polar lipid fatty acids	1727:1753	The polar lipid fatty acids	1727:1753	The polar lipid fatty acids were dominated by anteiso-C17 : 0 and iso-C16 : 0.
33999794	10	18	theme	polar	1374:1378	arg1	lipids					1380:1385	The identified polar lipids	1359:1385	The identified polar lipids	1359:1385	The identified polar lipids were represented by phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides.
33999794	5	19	theme	highest	759:765	arg1	similarity					776:785	the highest sequence similarity	755:785	the highest sequence similarity value to Glycomyces buryatensis 18T (92.1 %) and Salininema proteolyticum Miq-4T (91.8 %)	755:875	The 16S rRNA gene-based phylogenetic analysis demonstrated that strain ACPA22T formed a deep-branching lineage within the family Glycomycetaceae, with the highest sequence similarity value to Glycomyces buryatensis 18T (92.1 %) and Salininema proteolyticum Miq-4T (91.8 %).
33999794	6	20	theme	Glycomycetaceae	957:971	arg1	members					973:979	other Glycomycetaceae members	951:979	other Glycomycetaceae members	951:979	The average amino acid identity values (56.1-61.5 %) between ACPA22T and other Glycomycetaceae members with available genomes did not exceed the threshold reported for different genera.
33999794	4	21	theme	ACPA22T	466:472	arg1	nature					456:461	The hydrolytic nature	441:461	The hydrolytic nature of ACPA22T	441:472	The hydrolytic nature of ACPA22T was confirmed by two different growth-dependent methods and by the presence of multiple glycosidase-encoding genes in the genome.
33999794	7	22	contain	contained	1089:1097	arg2	alanine					1152:1158	alanine	1152:1158	alanine	1152:1158	The cell wall of ACPA22T contained meso-diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio, characteristic of the peptidoglycan type A1γ'.
33999794	7	22	contain	contained	1089:1097	arg2	acid					1119:1122	meso-diaminopimelic acid	1099:1122	meso-diaminopimelic acid	1099:1122	The cell wall of ACPA22T contained meso-diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio, characteristic of the peptidoglycan type A1γ'.
33999794	7	22	contain	contained	1089:1097	arg2	acid					1143:1146	glutamic acid	1134:1146	glutamic acid	1134:1146	The cell wall of ACPA22T contained meso-diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio, characteristic of the peptidoglycan type A1γ'.
33999794	7	22	contain	contained	1089:1097	arg1	wall					1073:1076	The cell wall	1064:1076	The cell wall of ACPA22T	1064:1087	The cell wall of ACPA22T contained meso-diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio, characteristic of the peptidoglycan type A1γ'.
33999794	7	22	contain	contained	1089:1097	arg2	glycine					1125:1131	glycine	1125:1131	glycine	1125:1131	The cell wall of ACPA22T contained meso-diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio, characteristic of the peptidoglycan type A1γ'.
33999794	7	23	from	acid	1143:1146	arg1	ratio					1171:1175	a molar ratio	1163:1175	a molar ratio	1163:1175	The cell wall of ACPA22T contained meso-diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio, characteristic of the peptidoglycan type A1γ'.
33999794	3	24	from	7.3-9.0	360:366	arg1	optimum					349:355	optimum	349:355	optimum	349:355	The isolate was facultatively alkaliphilic, growing at pH 6.5-10.5 (optimum at 7.3-9.0) and highly salt-tolerant, tolerating up to 3 M total Na+ as carbonates.
33999794	3	24	from	7.3-9.0	360:366	arg1	isolate					285:291	The isolate	281:291	The isolate	281:291	The isolate was facultatively alkaliphilic, growing at pH 6.5-10.5 (optimum at 7.3-9.0) and highly salt-tolerant, tolerating up to 3 M total Na+ as carbonates.
33999794	3	24	from	7.3-9.0	360:366	arg1	alkaliphilic					311:322	alkaliphilic	311:322	alkaliphilic	311:322	The isolate was facultatively alkaliphilic, growing at pH 6.5-10.5 (optimum at 7.3-9.0) and highly salt-tolerant, tolerating up to 3 M total Na+ as carbonates.
33999794	6	25	theme	different	1046:1054	arg1	genera					1056:1061	different genera	1046:1061	different genera	1046:1061	The average amino acid identity values (56.1-61.5 %) between ACPA22T and other Glycomycetaceae members with available genomes did not exceed the threshold reported for different genera.
33999794	7	26	from	alanine	1152:1158	arg1	ratio					1171:1175	a molar ratio	1163:1175	a molar ratio	1163:1175	The cell wall of ACPA22T contained meso-diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio, characteristic of the peptidoglycan type A1γ'.
33999794	13	27	theme	content	1856:1862	arg1	%					1872:1872	G+C content 61.5 mol%	1852:1872	G+C content 61.5 mol%	1852:1872	The genome included a chromosome of 3.94 Mbp (G+C content 61.5 mol%) encoding 3285 proteins and two plasmids of 59.8 and 14.8 kBp.
33999794	13	27	theme	content	1856:1862	arg1	Mbp					1847:1849	3.94 Mbp	1842:1849	3.94 Mbp (G+C content 61.5 mol%) encoding 3285 proteins	1842:1896	The genome included a chromosome of 3.94 Mbp (G+C content 61.5 mol%) encoding 3285 proteins and two plasmids of 59.8 and 14.8 kBp.
33999794	5	28	theme	buryatensis	807:817	arg1	18T					819:821	Glycomyces buryatensis 18T	796:821	Glycomyces buryatensis 18T (92.1 %)	796:830	The 16S rRNA gene-based phylogenetic analysis demonstrated that strain ACPA22T formed a deep-branching lineage within the family Glycomycetaceae, with the highest sequence similarity value to Glycomyces buryatensis 18T (92.1 %) and Salininema proteolyticum Miq-4T (91.8 %).
33999794	5	28	theme	buryatensis	807:817	arg1	%					829:829	92.1 %	824:829	92.1 %	824:829	The 16S rRNA gene-based phylogenetic analysis demonstrated that strain ACPA22T formed a deep-branching lineage within the family Glycomycetaceae, with the highest sequence similarity value to Glycomyces buryatensis 18T (92.1 %) and Salininema proteolyticum Miq-4T (91.8 %).
33999794	1	29	from	soil	101:104	arg1	actinobacterium					63:77	a haloalkaliphilic actinobacterium	44:77	a haloalkaliphilic actinobacterium from a soda solonchak soil	44:104	nov, a haloalkaliphilic actinobacterium from a soda solonchak soil.
33999794	1	29	from	soil	101:104	arg1	nov					39:41	nov	39:41	nov	39:41	nov, a haloalkaliphilic actinobacterium from a soda solonchak soil.
33999794	2	30	attach	isolated	187:194	arg2	ACPA22T					161:167	strain ACPA22T	154:167	strain ACPA22T	154:167	A haloalkaliphilic hydrolytic actinobacterium, strain ACPA22T, was enriched and isolated in pure culture from saline alkaline soil (soda solonchak) in northeastern Mongolia.
33999794	2	30	attach	isolated	187:194	arg2	actinobacterium					137:151	A haloalkaliphilic hydrolytic actinobacterium	107:151	A haloalkaliphilic hydrolytic actinobacterium	107:151	A haloalkaliphilic hydrolytic actinobacterium, strain ACPA22T, was enriched and isolated in pure culture from saline alkaline soil (soda solonchak) in northeastern Mongolia.
33999794	2	30	attach	isolated	187:194	arg1	soil					233:236	saline alkaline soil	217:236	saline alkaline soil (soda solonchak) in northeastern Mongolia	217:278	A haloalkaliphilic hydrolytic actinobacterium, strain ACPA22T, was enriched and isolated in pure culture from saline alkaline soil (soda solonchak) in northeastern Mongolia.
33999794	2	30	attach	isolated	187:194	arg1	solonchak					244:252	soda solonchak	239:252	soda solonchak	239:252	A haloalkaliphilic hydrolytic actinobacterium, strain ACPA22T, was enriched and isolated in pure culture from saline alkaline soil (soda solonchak) in northeastern Mongolia.
33999794	4	31	theme	growth-dependent	505:520	arg1	methods					522:528	two different growth-dependent methods	491:528	two different growth-dependent methods	491:528	The hydrolytic nature of ACPA22T was confirmed by two different growth-dependent methods and by the presence of multiple glycosidase-encoding genes in the genome.
33999794	14	32	theme	albus	2022:2026	arg1	genus					1985:1989	a new genus	1979:1989	a new genus	1979:1989	Based on the data obtained in this study, a new genus and species, Natronoglycomyces albus gen. nov., sp.
33999794	14	32	theme	albus	2022:2026	arg1	sp					2039:2040	sp	2039:2040	sp	2039:2040	Based on the data obtained in this study, a new genus and species, Natronoglycomyces albus gen. nov., sp.
33999794	14	32	theme	albus	2022:2026	arg1	nov.					2033:2036	Natronoglycomyces albus gen. nov.	2004:2036	Natronoglycomyces albus gen. nov.	2004:2036	Based on the data obtained in this study, a new genus and species, Natronoglycomyces albus gen. nov., sp.
33999794	0	33	theme	gen.	24:27	arg1	nov.					29:32	gen. nov.	24:32	gen. nov.	24:32	Natronoglycomyces albus gen. nov., sp.
33999794	1	34	theme	solonchak	91:99	arg1	soil					101:104	a soda solonchak soil	84:104	a soda solonchak soil	84:104	nov, a haloalkaliphilic actinobacterium from a soda solonchak soil.
33999794	5	35	theme	similarity	776:785	arg1	value					787:791	the highest sequence similarity value	755:791	the highest sequence similarity value to Glycomyces buryatensis 18T (92.1 %) and Salininema proteolyticum Miq-4T (91.8 %)	755:875	The 16S rRNA gene-based phylogenetic analysis demonstrated that strain ACPA22T formed a deep-branching lineage within the family Glycomycetaceae, with the highest sequence similarity value to Glycomyces buryatensis 18T (92.1 %) and Salininema proteolyticum Miq-4T (91.8 %).
33999794	9	36	theme	major	1310:1314	arg1	menaquinones					1316:1327	The major menaquinones	1306:1327	The major menaquinones	1306:1327	The major menaquinones were MK-10(Н4) and MK-11(Н4).
33999794	9	36	theme	major	1310:1314	arg1	MK-10					1334:1338	MK-10	1334:1338	MK-10	1334:1338	The major menaquinones were MK-10(Н4) and MK-11(Н4).
33999794	11	37	theme	unidentified	1571:1582	arg1	phospholipid					1643:1654	an amine-containing phospholipid	1623:1654	an amine-containing phospholipid with chromatographic mobility similar to that of phosphatidylinositol	1623:1724	In addition, the strain had a few unidentified characteristic polar lipids, including an amine-containing phospholipid with chromatographic mobility similar to that of phosphatidylinositol.
33999794	11	37	theme	unidentified	1571:1582	arg1	lipids					1605:1610	a few unidentified characteristic polar lipids	1565:1610	a few unidentified characteristic polar lipids	1565:1610	In addition, the strain had a few unidentified characteristic polar lipids, including an amine-containing phospholipid with chromatographic mobility similar to that of phosphatidylinositol.
33999794	3	38	dep	3	412:412	arg1	to					409:410	to	409:410	to	409:410	The isolate was facultatively alkaliphilic, growing at pH 6.5-10.5 (optimum at 7.3-9.0) and highly salt-tolerant, tolerating up to 3 M total Na+ as carbonates.
33999794	14	39	theme	new	1981:1983	arg1	genus					1985:1989	a new genus	1979:1989	a new genus	1979:1989	Based on the data obtained in this study, a new genus and species, Natronoglycomyces albus gen. nov., sp.
33999794	14	39	theme	new	1981:1983	arg1	data					1950:1953	the data	1946:1953	the data obtained in this study	1946:1976	Based on the data obtained in this study, a new genus and species, Natronoglycomyces albus gen. nov., sp.
33999794	14	39	theme	new	1981:1983	arg1	nov.					2033:2036	Natronoglycomyces albus gen. nov.	2004:2036	Natronoglycomyces albus gen. nov.	2004:2036	Based on the data obtained in this study, a new genus and species, Natronoglycomyces albus gen. nov., sp.
33999794	4	40	theme	glycosidase-encoding	562:581	arg1	genes					583:587	multiple glycosidase-encoding genes	553:587	multiple glycosidase-encoding genes	553:587	The hydrolytic nature of ACPA22T was confirmed by two different growth-dependent methods and by the presence of multiple glycosidase-encoding genes in the genome.
33999794	15	41	theme	=DSM	2090:2093	arg1	ACPA22T					2081:2087	the type strain ACPA22T	2065:2087	the type strain ACPA22T (=DSM 106290T=VKM Ac-2771T)	2065:2115	nov, is proposed with the type strain ACPA22T (=DSM 106290T=VKM Ac-2771T).
33999794	15	41	theme	=DSM	2090:2093	arg1	Ac-2771T					2107:2114	=DSM 106290T=VKM Ac-2771T	2090:2114	=DSM 106290T=VKM Ac-2771T	2090:2114	nov, is proposed with the type strain ACPA22T (=DSM 106290T=VKM Ac-2771T).
33999794	6	42	theme	acid	896:899	arg1	identity					901:908	The average amino acid identity	878:908	The average amino acid identity values (56.1-61.5 %) between ACPA22T and other Glycomycetaceae members with available genomes	878:1002	The average amino acid identity values (56.1-61.5 %) between ACPA22T and other Glycomycetaceae members with available genomes did not exceed the threshold reported for different genera.
33999794	6	42	theme	acid	896:899	arg1	%					928:928	56.1-61.5 %	918:928	56.1-61.5 %	918:928	The average amino acid identity values (56.1-61.5 %) between ACPA22T and other Glycomycetaceae members with available genomes did not exceed the threshold reported for different genera.
33999794	0	43	dep	Natronoglycomyces	0:16	arg1	nov.					29:32	gen. nov.	24:32	gen. nov.	24:32	Natronoglycomyces albus gen. nov., sp.
33999794	0	43	dep	Natronoglycomyces	0:16	arg1	albus					18:22	Natronoglycomyces albus gen. nov.,	0:33	albus	18:22	Natronoglycomyces albus gen. nov., sp.
33999794	5	44	theme	rRNA	612:615	arg1	analysis					641:648	The 16S rRNA gene-based phylogenetic analysis	604:648	The 16S rRNA gene-based phylogenetic analysis	604:648	The 16S rRNA gene-based phylogenetic analysis demonstrated that strain ACPA22T formed a deep-branching lineage within the family Glycomycetaceae, with the highest sequence similarity value to Glycomyces buryatensis 18T (92.1 %) and Salininema proteolyticum Miq-4T (91.8 %).
33999794	11	45	theme	polar	1599:1603	arg1	phospholipid					1643:1654	an amine-containing phospholipid	1623:1654	an amine-containing phospholipid with chromatographic mobility similar to that of phosphatidylinositol	1623:1724	In addition, the strain had a few unidentified characteristic polar lipids, including an amine-containing phospholipid with chromatographic mobility similar to that of phosphatidylinositol.
33999794	11	45	theme	polar	1599:1603	arg1	lipids					1605:1610	a few unidentified characteristic polar lipids	1565:1610	a few unidentified characteristic polar lipids	1565:1610	In addition, the strain had a few unidentified characteristic polar lipids, including an amine-containing phospholipid with chromatographic mobility similar to that of phosphatidylinositol.
33999794	6	46	theme	average	882:888	arg1	identity					901:908	The average amino acid identity	878:908	The average amino acid identity values (56.1-61.5 %) between ACPA22T and other Glycomycetaceae members with available genomes	878:1002	The average amino acid identity values (56.1-61.5 %) between ACPA22T and other Glycomycetaceae members with available genomes did not exceed the threshold reported for different genera.
33999794	6	46	theme	average	882:888	arg1	%					928:928	56.1-61.5 %	918:928	56.1-61.5 %	918:928	The average amino acid identity values (56.1-61.5 %) between ACPA22T and other Glycomycetaceae members with available genomes did not exceed the threshold reported for different genera.
33999794	7	47	theme	type	1214:1217	arg1	A1γ					1219:1221	the peptidoglycan type A1γ'	1196:1222	the peptidoglycan type A1γ'	1196:1222	The cell wall of ACPA22T contained meso-diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio, characteristic of the peptidoglycan type A1γ'.
33999794	5	48	theme	phylogenetic	628:639	arg1	analysis					641:648	The 16S rRNA gene-based phylogenetic analysis	604:648	The 16S rRNA gene-based phylogenetic analysis	604:648	The 16S rRNA gene-based phylogenetic analysis demonstrated that strain ACPA22T formed a deep-branching lineage within the family Glycomycetaceae, with the highest sequence similarity value to Glycomyces buryatensis 18T (92.1 %) and Salininema proteolyticum Miq-4T (91.8 %).
33999794	13	49	theme	kBp	1932:1934	arg1	plasmids					1906:1913	two plasmids	1902:1913	two plasmids of 59.8 and 14.8 kBp	1902:1934	The genome included a chromosome of 3.94 Mbp (G+C content 61.5 mol%) encoding 3285 proteins and two plasmids of 59.8 and 14.8 kBp.
33999794	13	49	theme	kBp	1932:1934	arg1	chromosome					1828:1837	a chromosome	1826:1837	a chromosome of 3.94 Mbp (G+C content 61.5 mol%) encoding 3285 proteins	1826:1896	The genome included a chromosome of 3.94 Mbp (G+C content 61.5 mol%) encoding 3285 proteins and two plasmids of 59.8 and 14.8 kBp.
33999794	11	50	with	phospholipid	1643:1654	arg1	mobility					1677:1684	chromatographic mobility	1661:1684	chromatographic mobility similar to that of phosphatidylinositol	1661:1724	In addition, the strain had a few unidentified characteristic polar lipids, including an amine-containing phospholipid with chromatographic mobility similar to that of phosphatidylinositol.
33999794	11	51	theme	similar	1686:1692	arg1	mobility					1677:1684	chromatographic mobility	1661:1684	chromatographic mobility similar to that of phosphatidylinositol	1661:1724	In addition, the strain had a few unidentified characteristic polar lipids, including an amine-containing phospholipid with chromatographic mobility similar to that of phosphatidylinositol.
33999794	7	52	theme	cell	1068:1071	arg1	wall					1073:1076	The cell wall	1064:1076	The cell wall of ACPA22T	1064:1087	The cell wall of ACPA22T contained meso-diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio, characteristic of the peptidoglycan type A1γ'.
33999794	5	53	theme	Salininema	836:845	arg1	Miq-4T					861:866	Salininema proteolyticum Miq-4T	836:866	Salininema proteolyticum Miq-4T (91.8 %)	836:875	The 16S rRNA gene-based phylogenetic analysis demonstrated that strain ACPA22T formed a deep-branching lineage within the family Glycomycetaceae, with the highest sequence similarity value to Glycomyces buryatensis 18T (92.1 %) and Salininema proteolyticum Miq-4T (91.8 %).
33999794	5	53	theme	Salininema	836:845	arg1	%					874:874	91.8 %	869:874	91.8 %	869:874	The 16S rRNA gene-based phylogenetic analysis demonstrated that strain ACPA22T formed a deep-branching lineage within the family Glycomycetaceae, with the highest sequence similarity value to Glycomyces buryatensis 18T (92.1 %) and Salininema proteolyticum Miq-4T (91.8 %).
33999794	11	54	theme	chromatographic	1661:1675	arg1	mobility					1677:1684	chromatographic mobility	1661:1684	chromatographic mobility similar to that of phosphatidylinositol	1661:1724	In addition, the strain had a few unidentified characteristic polar lipids, including an amine-containing phospholipid with chromatographic mobility similar to that of phosphatidylinositol.
33999794	7	55	theme	characteristic	1178:1191	arg1	ratio					1171:1175	a molar ratio	1163:1175	a molar ratio	1163:1175	The cell wall of ACPA22T contained meso-diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio, characteristic of the peptidoglycan type A1γ'.
33999794	15	56	theme	strain	2074:2079	arg1	ACPA22T					2081:2087	the type strain ACPA22T	2065:2087	the type strain ACPA22T (=DSM 106290T=VKM Ac-2771T)	2065:2115	nov, is proposed with the type strain ACPA22T (=DSM 106290T=VKM Ac-2771T).
33999794	15	56	theme	strain	2074:2079	arg1	Ac-2771T					2107:2114	=DSM 106290T=VKM Ac-2771T	2090:2114	=DSM 106290T=VKM Ac-2771T	2090:2114	nov, is proposed with the type strain ACPA22T (=DSM 106290T=VKM Ac-2771T).
33999794	6	57	with	identity	901:908	arg1	genomes					996:1002	available genomes	986:1002	available genomes	986:1002	The average amino acid identity values (56.1-61.5 %) between ACPA22T and other Glycomycetaceae members with available genomes did not exceed the threshold reported for different genera.
33999794	5	58	theme	deep-branching	692:705	arg1	lineage					707:713	a deep-branching lineage	690:713	a deep-branching lineage	690:713	The 16S rRNA gene-based phylogenetic analysis demonstrated that strain ACPA22T formed a deep-branching lineage within the family Glycomycetaceae, with the highest sequence similarity value to Glycomyces buryatensis 18T (92.1 %) and Salininema proteolyticum Miq-4T (91.8 %).
33999794	2	59	theme	alkaline	224:231	arg1	soil					233:236	saline alkaline soil	217:236	saline alkaline soil (soda solonchak) in northeastern Mongolia	217:278	A haloalkaliphilic hydrolytic actinobacterium, strain ACPA22T, was enriched and isolated in pure culture from saline alkaline soil (soda solonchak) in northeastern Mongolia.
33999794	2	59	theme	alkaline	224:231	arg1	solonchak					244:252	soda solonchak	239:252	soda solonchak	239:252	A haloalkaliphilic hydrolytic actinobacterium, strain ACPA22T, was enriched and isolated in pure culture from saline alkaline soil (soda solonchak) in northeastern Mongolia.
33999794	8	60	theme	whole-cell	1229:1238	arg1	sugars					1240:1245	The whole-cell sugars	1225:1245	The whole-cell sugars	1225:1245	The whole-cell sugars included mannose, galactose, arabinose, ribose and xylose.
33999794	5	61	theme	strain	668:673	arg1	ACPA22T					675:681	strain ACPA22T	668:681	strain ACPA22T	668:681	The 16S rRNA gene-based phylogenetic analysis demonstrated that strain ACPA22T formed a deep-branching lineage within the family Glycomycetaceae, with the highest sequence similarity value to Glycomyces buryatensis 18T (92.1 %) and Salininema proteolyticum Miq-4T (91.8 %).
33999794	12	62	theme	polar	1731:1735	arg1	acids					1749:1753	The polar lipid fatty acids	1727:1753	The polar lipid fatty acids	1727:1753	The polar lipid fatty acids were dominated by anteiso-C17 : 0 and iso-C16 : 0.
33999794	6	63	theme	available	986:994	arg1	genomes					996:1002	available genomes	986:1002	available genomes	986:1002	The average amino acid identity values (56.1-61.5 %) between ACPA22T and other Glycomycetaceae members with available genomes did not exceed the threshold reported for different genera.
33999794	7	64	from	acid	1119:1122	arg1	ratio					1171:1175	a molar ratio	1163:1175	a molar ratio	1163:1175	The cell wall of ACPA22T contained meso-diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio, characteristic of the peptidoglycan type A1γ'.
33999794	13	65	theme	61.5 mol	1864:1871	arg1	%					1872:1872	G+C content 61.5 mol%	1852:1872	G+C content 61.5 mol%	1852:1872	The genome included a chromosome of 3.94 Mbp (G+C content 61.5 mol%) encoding 3285 proteins and two plasmids of 59.8 and 14.8 kBp.
33999794	13	65	theme	61.5 mol	1864:1871	arg1	Mbp					1847:1849	3.94 Mbp	1842:1849	3.94 Mbp (G+C content 61.5 mol%) encoding 3285 proteins	1842:1896	The genome included a chromosome of 3.94 Mbp (G+C content 61.5 mol%) encoding 3285 proteins and two plasmids of 59.8 and 14.8 kBp.
33999794	2	66	theme	soda	239:242	arg1	soil					233:236	saline alkaline soil	217:236	saline alkaline soil (soda solonchak) in northeastern Mongolia	217:278	A haloalkaliphilic hydrolytic actinobacterium, strain ACPA22T, was enriched and isolated in pure culture from saline alkaline soil (soda solonchak) in northeastern Mongolia.
33999794	2	66	theme	soda	239:242	arg1	solonchak					244:252	soda solonchak	239:252	soda solonchak	239:252	A haloalkaliphilic hydrolytic actinobacterium, strain ACPA22T, was enriched and isolated in pure culture from saline alkaline soil (soda solonchak) in northeastern Mongolia.
33999794	15	67	theme	106290T=VKM	2095:2105	arg1	ACPA22T					2081:2087	the type strain ACPA22T	2065:2087	the type strain ACPA22T (=DSM 106290T=VKM Ac-2771T)	2065:2115	nov, is proposed with the type strain ACPA22T (=DSM 106290T=VKM Ac-2771T).
33999794	15	67	theme	106290T=VKM	2095:2105	arg1	Ac-2771T					2107:2114	=DSM 106290T=VKM Ac-2771T	2090:2114	=DSM 106290T=VKM Ac-2771T	2090:2114	nov, is proposed with the type strain ACPA22T (=DSM 106290T=VKM Ac-2771T).
33999794	7	68	from	glycine	1125:1131	arg1	ratio					1171:1175	a molar ratio	1163:1175	a molar ratio	1163:1175	The cell wall of ACPA22T contained meso-diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio, characteristic of the peptidoglycan type A1γ'.
33999794	10	69	theme	identified	1363:1372	arg1	lipids					1380:1385	The identified polar lipids	1359:1385	The identified polar lipids	1359:1385	The identified polar lipids were represented by phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannosides.
33999794	12	70	dep	anteiso-C17 	1773:1784	arg1	 0					1802:1803	 0	1802:1803	anteiso-C17 : 0 and iso-C16 : 0	1773:1803	The polar lipid fatty acids were dominated by anteiso-C17 : 0 and iso-C16 : 0.
33999794	12	70	dep	anteiso-C17 	1773:1784	arg1	iso-C16 					1793:1800	iso-C16 	1793:1800	iso-C16 	1793:1800	The polar lipid fatty acids were dominated by anteiso-C17 : 0 and iso-C16 : 0.
33999794	12	70	dep	anteiso-C17 	1773:1784	arg1	 0					1786:1787	 0	1786:1787	 0	1786:1787	The polar lipid fatty acids were dominated by anteiso-C17 : 0 and iso-C16 : 0.
33999794	7	71	theme	molar	1165:1169	arg1	ratio					1171:1175	a molar ratio	1163:1175	a molar ratio	1163:1175	The cell wall of ACPA22T contained meso-diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio, characteristic of the peptidoglycan type A1γ'.
33999794	6	72	theme	other	951:955	arg1	members					973:979	other Glycomycetaceae members	951:979	other Glycomycetaceae members	951:979	The average amino acid identity values (56.1-61.5 %) between ACPA22T and other Glycomycetaceae members with available genomes did not exceed the threshold reported for different genera.
33999794	13	73	theme	Mbp	1847:1849	arg1	plasmids					1906:1913	two plasmids	1902:1913	two plasmids of 59.8 and 14.8 kBp	1902:1934	The genome included a chromosome of 3.94 Mbp (G+C content 61.5 mol%) encoding 3285 proteins and two plasmids of 59.8 and 14.8 kBp.
33999794	13	73	theme	Mbp	1847:1849	arg1	chromosome					1828:1837	a chromosome	1826:1837	a chromosome of 3.94 Mbp (G+C content 61.5 mol%) encoding 3285 proteins	1826:1896	The genome included a chromosome of 3.94 Mbp (G+C content 61.5 mol%) encoding 3285 proteins and two plasmids of 59.8 and 14.8 kBp.
33999794	4	74	from	presence	541:548	arg1	genome					596:601	the genome	592:601	the genome	592:601	The hydrolytic nature of ACPA22T was confirmed by two different growth-dependent methods and by the presence of multiple glycosidase-encoding genes in the genome.
33999794	1	75	theme	haloalkaliphilic	46:61	arg1	actinobacterium					63:77	a haloalkaliphilic actinobacterium	44:77	a haloalkaliphilic actinobacterium from a soda solonchak soil	44:104	nov, a haloalkaliphilic actinobacterium from a soda solonchak soil.
33999794	1	75	theme	haloalkaliphilic	46:61	arg1	nov					39:41	nov	39:41	nov	39:41	nov, a haloalkaliphilic actinobacterium from a soda solonchak soil.
33999794	4	76	theme	hydrolytic	445:454	arg1	nature					456:461	The hydrolytic nature	441:461	The hydrolytic nature of ACPA22T	441:472	The hydrolytic nature of ACPA22T was confirmed by two different growth-dependent methods and by the presence of multiple glycosidase-encoding genes in the genome.
33999794	13	77	theme	G+C	1852:1854	arg1	%					1872:1872	G+C content 61.5 mol%	1852:1872	G+C content 61.5 mol%	1852:1872	The genome included a chromosome of 3.94 Mbp (G+C content 61.5 mol%) encoding 3285 proteins and two plasmids of 59.8 and 14.8 kBp.
33999794	13	77	theme	G+C	1852:1854	arg1	Mbp					1847:1849	3.94 Mbp	1842:1849	3.94 Mbp (G+C content 61.5 mol%) encoding 3285 proteins	1842:1896	The genome included a chromosome of 3.94 Mbp (G+C content 61.5 mol%) encoding 3285 proteins and two plasmids of 59.8 and 14.8 kBp.
33999794	5	78	theme	family	726:731	arg1	Glycomycetaceae					733:747	the family Glycomycetaceae	722:747	the family Glycomycetaceae	722:747	The 16S rRNA gene-based phylogenetic analysis demonstrated that strain ACPA22T formed a deep-branching lineage within the family Glycomycetaceae, with the highest sequence similarity value to Glycomyces buryatensis 18T (92.1 %) and Salininema proteolyticum Miq-4T (91.8 %).
33999794	5	79	theme	Glycomyces	796:805	arg1	18T					819:821	Glycomyces buryatensis 18T	796:821	Glycomyces buryatensis 18T (92.1 %)	796:830	The 16S rRNA gene-based phylogenetic analysis demonstrated that strain ACPA22T formed a deep-branching lineage within the family Glycomycetaceae, with the highest sequence similarity value to Glycomyces buryatensis 18T (92.1 %) and Salininema proteolyticum Miq-4T (91.8 %).
33999794	5	79	theme	Glycomyces	796:805	arg1	%					829:829	92.1 %	824:829	92.1 %	824:829	The 16S rRNA gene-based phylogenetic analysis demonstrated that strain ACPA22T formed a deep-branching lineage within the family Glycomycetaceae, with the highest sequence similarity value to Glycomyces buryatensis 18T (92.1 %) and Salininema proteolyticum Miq-4T (91.8 %).
33999794	14	80	theme	gen.	2028:2031	arg1	genus					1985:1989	a new genus	1979:1989	a new genus	1979:1989	Based on the data obtained in this study, a new genus and species, Natronoglycomyces albus gen. nov., sp.
33999794	14	80	theme	gen.	2028:2031	arg1	sp					2039:2040	sp	2039:2040	sp	2039:2040	Based on the data obtained in this study, a new genus and species, Natronoglycomyces albus gen. nov., sp.
33999794	14	80	theme	gen.	2028:2031	arg1	nov.					2033:2036	Natronoglycomyces albus gen. nov.	2004:2036	Natronoglycomyces albus gen. nov.	2004:2036	Based on the data obtained in this study, a new genus and species, Natronoglycomyces albus gen. nov., sp.
33999794	7	81	theme	glutamic	1134:1141	arg1	acid					1143:1146	glutamic acid	1134:1146	glutamic acid	1134:1146	The cell wall of ACPA22T contained meso-diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio, characteristic of the peptidoglycan type A1γ'.
33999794	1	82	theme	soda	86:89	arg1	soil					101:104	a soda solonchak soil	84:104	a soda solonchak soil	84:104	nov, a haloalkaliphilic actinobacterium from a soda solonchak soil.
33999794	2	83	from	soil	233:236	arg1	Mongolia					271:278	Mongolia	271:278	Mongolia	271:278	A haloalkaliphilic hydrolytic actinobacterium, strain ACPA22T, was enriched and isolated in pure culture from saline alkaline soil (soda solonchak) in northeastern Mongolia.
33999794	3	84	dep	alkaliphilic	311:322	arg1	optimum					349:355	optimum	349:355	optimum	349:355	The isolate was facultatively alkaliphilic, growing at pH 6.5-10.5 (optimum at 7.3-9.0) and highly salt-tolerant, tolerating up to 3 M total Na+ as carbonates.
33999794	3	84	dep	alkaliphilic	311:322	arg1	isolate					285:291	The isolate	281:291	The isolate	281:291	The isolate was facultatively alkaliphilic, growing at pH 6.5-10.5 (optimum at 7.3-9.0) and highly salt-tolerant, tolerating up to 3 M total Na+ as carbonates.
33999794	3	84	dep	alkaliphilic	311:322	arg1	alkaliphilic					311:322	alkaliphilic	311:322	alkaliphilic	311:322	The isolate was facultatively alkaliphilic, growing at pH 6.5-10.5 (optimum at 7.3-9.0) and highly salt-tolerant, tolerating up to 3 M total Na+ as carbonates.
33999794	5	85	theme	sequence	767:774	arg1	similarity					776:785	the highest sequence similarity	755:785	the highest sequence similarity value to Glycomyces buryatensis 18T (92.1 %) and Salininema proteolyticum Miq-4T (91.8 %)	755:875	The 16S rRNA gene-based phylogenetic analysis demonstrated that strain ACPA22T formed a deep-branching lineage within the family Glycomycetaceae, with the highest sequence similarity value to Glycomyces buryatensis 18T (92.1 %) and Salininema proteolyticum Miq-4T (91.8 %).
33999794	2	86	theme	hydrolytic	126:135	arg1	actinobacterium					137:151	A haloalkaliphilic hydrolytic actinobacterium	107:151	A haloalkaliphilic hydrolytic actinobacterium	107:151	A haloalkaliphilic hydrolytic actinobacterium, strain ACPA22T, was enriched and isolated in pure culture from saline alkaline soil (soda solonchak) in northeastern Mongolia.
33999794	2	86	theme	hydrolytic	126:135	arg1	ACPA22T					161:167	strain ACPA22T	154:167	strain ACPA22T	154:167	A haloalkaliphilic hydrolytic actinobacterium, strain ACPA22T, was enriched and isolated in pure culture from saline alkaline soil (soda solonchak) in northeastern Mongolia.
33999794	11	87	theme	few	1567:1569	arg1	phospholipid					1643:1654	an amine-containing phospholipid	1623:1654	an amine-containing phospholipid with chromatographic mobility similar to that of phosphatidylinositol	1623:1724	In addition, the strain had a few unidentified characteristic polar lipids, including an amine-containing phospholipid with chromatographic mobility similar to that of phosphatidylinositol.
33999794	11	87	theme	few	1567:1569	arg1	lipids					1605:1610	a few unidentified characteristic polar lipids	1565:1610	a few unidentified characteristic polar lipids	1565:1610	In addition, the strain had a few unidentified characteristic polar lipids, including an amine-containing phospholipid with chromatographic mobility similar to that of phosphatidylinositol.
33999794	3	88	theme	total	416:420	arg1	carbonates					429:438	carbonates	429:438	carbonates	429:438	The isolate was facultatively alkaliphilic, growing at pH 6.5-10.5 (optimum at 7.3-9.0) and highly salt-tolerant, tolerating up to 3 M total Na+ as carbonates.
33999794	3	88	theme	total	416:420	arg1	Na+					422:424	up to 3 M total Na+	406:424	up to 3 M total Na+	406:424	The isolate was facultatively alkaliphilic, growing at pH 6.5-10.5 (optimum at 7.3-9.0) and highly salt-tolerant, tolerating up to 3 M total Na+ as carbonates.
33999794	0	89	dep	sp	35:36	arg1	Natronoglycomyces					0:16	Natronoglycomyces	0:16	Natronoglycomyces	0:16	Natronoglycomyces albus gen. nov., sp.
33999794	4	90	theme	different	495:503	arg1	methods					522:528	two different growth-dependent methods	491:528	two different growth-dependent methods	491:528	The hydrolytic nature of ACPA22T was confirmed by two different growth-dependent methods and by the presence of multiple glycosidase-encoding genes in the genome.
33999794	2	91	theme	haloalkaliphilic	109:124	arg1	actinobacterium					137:151	A haloalkaliphilic hydrolytic actinobacterium	107:151	A haloalkaliphilic hydrolytic actinobacterium	107:151	A haloalkaliphilic hydrolytic actinobacterium, strain ACPA22T, was enriched and isolated in pure culture from saline alkaline soil (soda solonchak) in northeastern Mongolia.
33999794	2	91	theme	haloalkaliphilic	109:124	arg1	ACPA22T					161:167	strain ACPA22T	154:167	strain ACPA22T	154:167	A haloalkaliphilic hydrolytic actinobacterium, strain ACPA22T, was enriched and isolated in pure culture from saline alkaline soil (soda solonchak) in northeastern Mongolia.
33999794	2	92	theme	pure	199:202	arg1	culture					204:210	pure culture	199:210	pure culture	199:210	A haloalkaliphilic hydrolytic actinobacterium, strain ACPA22T, was enriched and isolated in pure culture from saline alkaline soil (soda solonchak) in northeastern Mongolia.
33999794	3	93	dep	M	414:414	arg1	3					412:412	3	412:412	3	412:412	The isolate was facultatively alkaliphilic, growing at pH 6.5-10.5 (optimum at 7.3-9.0) and highly salt-tolerant, tolerating up to 3 M total Na+ as carbonates.
33999794	4	94	theme	genes	583:587	arg1	presence					541:548	the presence	537:548	the presence of multiple glycosidase-encoding genes in the genome	537:601	The hydrolytic nature of ACPA22T was confirmed by two different growth-dependent methods and by the presence of multiple glycosidase-encoding genes in the genome.
33999794	3	95	dep	Na+	422:424	arg1	M					414:414	M	414:414	M	414:414	The isolate was facultatively alkaliphilic, growing at pH 6.5-10.5 (optimum at 7.3-9.0) and highly salt-tolerant, tolerating up to 3 M total Na+ as carbonates.
33999794	7	96	theme	A1γ	1219:1221	arg1	characteristic					1178:1191	characteristic	1178:1191	characteristic	1178:1191	The cell wall of ACPA22T contained meso-diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio, characteristic of the peptidoglycan type A1γ'.
33999794	11	97	theme	amine-containing	1626:1641	arg1	phospholipid					1643:1654	an amine-containing phospholipid	1623:1654	an amine-containing phospholipid with chromatographic mobility similar to that of phosphatidylinositol	1623:1724	In addition, the strain had a few unidentified characteristic polar lipids, including an amine-containing phospholipid with chromatographic mobility similar to that of phosphatidylinositol.
33999794	11	98	theme	characteristic	1584:1597	arg1	phospholipid					1643:1654	an amine-containing phospholipid	1623:1654	an amine-containing phospholipid with chromatographic mobility similar to that of phosphatidylinositol	1623:1724	In addition, the strain had a few unidentified characteristic polar lipids, including an amine-containing phospholipid with chromatographic mobility similar to that of phosphatidylinositol.
33999794	11	98	theme	characteristic	1584:1597	arg1	lipids					1605:1610	a few unidentified characteristic polar lipids	1565:1610	a few unidentified characteristic polar lipids	1565:1610	In addition, the strain had a few unidentified characteristic polar lipids, including an amine-containing phospholipid with chromatographic mobility similar to that of phosphatidylinositol.
34705623	6	0	theme	71.3	1203:1206	arg1	mol					1208:1210	mol	1208:1210	mol	1208:1210	The genome size of strain 14C53T was approximately 9.0 Mb and the genomic DNA G+C content of the strain was 71.3 mol%.
34705623	2	1	from	sample	157:162	arg1	material					176:183	basaltic material	167:183	basaltic material from Samsun, Turkey	167:203	An actinobacterium, designated 14C53T, was isolated from a soil sample on basaltic material from Samsun, Turkey.
34705623	6	2	theme	strain	1114:1119	arg1	14C53T					1121:1126	strain 14C53T	1114:1126	strain 14C53T	1114:1126	The genome size of strain 14C53T was approximately 9.0 Mb and the genomic DNA G+C content of the strain was 71.3 mol%.
34705623	6	3	theme	strain	1192:1197	arg1	%					1211:1211	71.3 mol%	1203:1211	71.3 mol%	1203:1211	The genome size of strain 14C53T was approximately 9.0 Mb and the genomic DNA G+C content of the strain was 71.3 mol%.
34705623	6	3	theme	strain	1192:1197	arg1	content					1177:1183	the genomic DNA G+C content	1157:1183	the genomic DNA G+C content of the strain	1157:1197	The genome size of strain 14C53T was approximately 9.0 Mb and the genomic DNA G+C content of the strain was 71.3 mol%.
34705623	3	4	with	clade	585:589	arg1	D310AT					622:627	Actinomadura alkaliterrae D310AT	596:627	Actinomadura alkaliterrae D310AT	596:627	The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT.
34705623	3	5	with	°C	347:348	arg1	optimum					359:365	an optimum	356:365	an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT	356:627	The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT.
34705623	3	6	theme	alkaliterrae	609:620	arg1	D310AT					622:627	Actinomadura alkaliterrae D310AT	596:627	Actinomadura alkaliterrae D310AT	596:627	The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT.
34705623	6	7	theme	G+C	1173:1175	arg1	%					1211:1211	71.3 mol%	1203:1211	71.3 mol%	1203:1211	The genome size of strain 14C53T was approximately 9.0 Mb and the genomic DNA G+C content of the strain was 71.3 mol%.
34705623	6	7	theme	G+C	1173:1175	arg1	content					1177:1183	the genomic DNA G+C content	1157:1183	the genomic DNA G+C content of the strain	1157:1197	The genome size of strain 14C53T was approximately 9.0 Mb and the genomic DNA G+C content of the strain was 71.3 mol%.
34705623	5	8	theme	nucleotide	790:799	arg1	identity					801:808	average nucleotide identity	782:808	average nucleotide identity	782:808	However, the digital DNA-DNA hybridization and average nucleotide identity values between strain 14C53T with Actinomadura geliboluensis A8036T and Actinomadura pelletieri DSM 43383T were 28.6-30.2 % and 84.3-85.5 %, respectively, and comparative analyses based on the genome sequences demonstrated that it represents a novel species of the genus Actinomadura.
34705623	3	9	theme	NaCl	228:231	arg1	concentration					233:245	NaCl concentration	228:245	NaCl concentration	228:245	The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT.
34705623	11	10	theme	type	1638:1641	arg1	strain					1643:1648	the type strain	1634:1648	the type strain	1634:1648	nov. is proposed, with 14C53T (=DSM 104447T=KCTC 39878T) as the type strain.
34705623	6	11	theme	DNA	1169:1171	arg1	%					1211:1211	71.3 mol%	1203:1211	71.3 mol%	1203:1211	The genome size of strain 14C53T was approximately 9.0 Mb and the genomic DNA G+C content of the strain was 71.3 mol%.
34705623	6	11	theme	DNA	1169:1171	arg1	content					1177:1183	the genomic DNA G+C content	1157:1183	the genomic DNA G+C content of the strain	1157:1197	The genome size of strain 14C53T was approximately 9.0 Mb and the genomic DNA G+C content of the strain was 71.3 mol%.
34705623	3	12	theme	%	522:522	arg1	value					535:539	98.5 % similarity value	517:539	98.5 % similarity value	517:539	The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT.
34705623	3	12	theme	%	522:522	arg1	A8036T					509:514	A8036T	509:514	A8036T	509:514	The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT.
34705623	7	13	dep	C16 	1267:1270	arg1	 0					1272:1273	 0	1272:1273	 0	1272:1273	The major cellular fatty acids of strain 14C53T were C16 : 0 and iso-C16 : 0.
34705623	7	13	dep	C16 	1267:1270	arg1	 0					1288:1289	 0	1288:1289	C16 : 0 and iso-C16 : 0	1267:1289	The major cellular fatty acids of strain 14C53T were C16 : 0 and iso-C16 : 0.
34705623	7	13	dep	C16 	1267:1270	arg1	iso-C16 					1279:1286	iso-C16 	1279:1286	iso-C16 	1279:1286	The major cellular fatty acids of strain 14C53T were C16 : 0 and iso-C16 : 0.
34705623	8	14	theme	diamino	1348:1354	arg1	acid					1356:1359	the diamino acid	1344:1359	the diamino acid in the cell-wall peptidoglycan	1344:1390	Strain 14C53T contained meso-diaminopimelic acid as the diamino acid in the cell-wall peptidoglycan.
34705623	8	14	theme	diamino	1348:1354	arg1	acid					1336:1339	meso-diaminopimelic acid	1316:1339	meso-diaminopimelic acid	1316:1339	Strain 14C53T contained meso-diaminopimelic acid as the diamino acid in the cell-wall peptidoglycan.
34705623	5	15	theme	comparative	969:979	arg1	analyses					981:988	comparative analyses	969:988	comparative analyses based on the genome sequences	969:1018	However, the digital DNA-DNA hybridization and average nucleotide identity values between strain 14C53T with Actinomadura geliboluensis A8036T and Actinomadura pelletieri DSM 43383T were 28.6-30.2 % and 84.3-85.5 %, respectively, and comparative analyses based on the genome sequences demonstrated that it represents a novel species of the genus Actinomadura.
34705623	11	16	theme	=DSM	1605:1608	arg1	14C53T					1597:1602	14C53T	1597:1602	14C53T (=DSM 104447T=KCTC 39878T) as the type strain	1597:1648	nov. is proposed, with 14C53T (=DSM 104447T=KCTC 39878T) as the type strain.
34705623	11	16	theme	=DSM	1605:1608	arg1	39878T					1623:1628	=DSM 104447T=KCTC 39878T	1605:1628	=DSM 104447T=KCTC 39878T	1605:1628	nov. is proposed, with 14C53T (=DSM 104447T=KCTC 39878T) as the type strain.
34705623	8	17	theme	cell-wall	1368:1376	arg1	peptidoglycan					1378:1390	the cell-wall peptidoglycan	1364:1390	the cell-wall peptidoglycan	1364:1390	Strain 14C53T contained meso-diaminopimelic acid as the diamino acid in the cell-wall peptidoglycan.
34705623	7	18	theme	strain	1248:1253	arg1	14C53T					1255:1260	strain 14C53T	1248:1260	strain 14C53T	1248:1260	The major cellular fatty acids of strain 14C53T were C16 : 0 and iso-C16 : 0.
34705623	10	19	theme	Actinomadura	1552:1563	arg1	sp					1570:1571	Actinomadura soli sp	1552:1571	a novel species Actinomadura soli sp	1536:1571	Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, a novel species Actinomadura soli sp.
34705623	5	20	theme	novel	1054:1058	arg1	species					1060:1066	a novel species	1052:1066	a novel species of the genus Actinomadura	1052:1092	However, the digital DNA-DNA hybridization and average nucleotide identity values between strain 14C53T with Actinomadura geliboluensis A8036T and Actinomadura pelletieri DSM 43383T were 28.6-30.2 % and 84.3-85.5 %, respectively, and comparative analyses based on the genome sequences demonstrated that it represents a novel species of the genus Actinomadura.
34705623	3	21	theme	14C53T	264:269	arg1	ranges					217:222	The growth ranges	206:222	The growth ranges for NaCl concentration and pH of strain 14C53T	206:269	The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT.
34705623	5	22	dep	A8036T	871:876	arg1	43383T					910:915	DSM 43383T	906:915	DSM 43383T	906:915	However, the digital DNA-DNA hybridization and average nucleotide identity values between strain 14C53T with Actinomadura geliboluensis A8036T and Actinomadura pelletieri DSM 43383T were 28.6-30.2 % and 84.3-85.5 %, respectively, and comparative analyses based on the genome sequences demonstrated that it represents a novel species of the genus Actinomadura.
34705623	5	23	theme	strain	825:830	arg1	14C53T					832:837	strain 14C53T	825:837	strain 14C53T with Actinomadura geliboluensis A8036T and Actinomadura pelletieri DSM 43383T	825:915	However, the digital DNA-DNA hybridization and average nucleotide identity values between strain 14C53T with Actinomadura geliboluensis A8036T and Actinomadura pelletieri DSM 43383T were 28.6-30.2 % and 84.3-85.5 %, respectively, and comparative analyses based on the genome sequences demonstrated that it represents a novel species of the genus Actinomadura.
34705623	3	24	theme	growth	298:303	arg1	range					317:321	the growth temperature range	294:321	the growth temperature range of the strain	294:335	The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT.
34705623	3	24	theme	growth	298:303	arg1	°C					347:348	20-37 °C	341:348	20-37 °C	341:348	The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT.
34705623	1	25	theme	top	46:48	arg1	layer					55:59	the top soil layer	42:59	the top soil layer on basaltic material in Turkey	42:90	nov., isolated from the top soil layer on basaltic material in Turkey.
34705623	5	26	with	14C53T	832:837	arg1	A8036T					871:876	A8036T	871:876	A8036T	871:876	However, the digital DNA-DNA hybridization and average nucleotide identity values between strain 14C53T with Actinomadura geliboluensis A8036T and Actinomadura pelletieri DSM 43383T were 28.6-30.2 % and 84.3-85.5 %, respectively, and comparative analyses based on the genome sequences demonstrated that it represents a novel species of the genus Actinomadura.
34705623	5	26	with	14C53T	832:837	arg1	pelletieri					895:904	pelletieri	895:904	pelletieri	895:904	However, the digital DNA-DNA hybridization and average nucleotide identity values between strain 14C53T with Actinomadura geliboluensis A8036T and Actinomadura pelletieri DSM 43383T were 28.6-30.2 % and 84.3-85.5 %, respectively, and comparative analyses based on the genome sequences demonstrated that it represents a novel species of the genus Actinomadura.
34705623	10	27	theme	phylogenetic	1513:1524	arg1	analyses					1526:1533	the phenotypic, genotypic and phylogenetic analyses	1483:1533	the phenotypic, genotypic and phylogenetic analyses	1483:1533	Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, a novel species Actinomadura soli sp.
34705623	10	27	theme	phylogenetic	1513:1524	arg1	species					1544:1550	a novel species Actinomadura soli sp	1536:1571	a novel species Actinomadura soli sp	1536:1571	Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, a novel species Actinomadura soli sp.
34705623	0	28	theme	Actinomadura	0:11	arg1	sp					18:19	Actinomadura soli sp	0:19	Actinomadura soli sp.	0:20	Actinomadura soli sp.
34705623	1	29	theme	basaltic	64:71	arg1	material					73:80	basaltic material	64:80	basaltic material in Turkey	64:90	nov., isolated from the top soil layer on basaltic material in Turkey.
34705623	7	30	theme	fatty	1233:1237	arg1	C16 					1267:1270	C16 	1267:1270	C16 	1267:1270	The major cellular fatty acids of strain 14C53T were C16 : 0 and iso-C16 : 0.
34705623	7	30	theme	fatty	1233:1237	arg1	acids					1239:1243	The major cellular fatty acids	1214:1243	The major cellular fatty acids of strain 14C53T	1214:1260	The major cellular fatty acids of strain 14C53T were C16 : 0 and iso-C16 : 0.
34705623	3	31	theme	Phylogenetic	377:388	arg1	analysis					390:397	Phylogenetic analysis	377:397	Phylogenetic analysis of 16S rRNA gene sequences	377:424	The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT.
34705623	7	32	theme	major	1218:1222	arg1	C16 					1267:1270	C16 	1267:1270	C16 	1267:1270	The major cellular fatty acids of strain 14C53T were C16 : 0 and iso-C16 : 0.
34705623	7	32	theme	major	1218:1222	arg1	acids					1239:1243	The major cellular fatty acids	1214:1243	The major cellular fatty acids of strain 14C53T	1214:1260	The major cellular fatty acids of strain 14C53T were C16 : 0 and iso-C16 : 0.
34705623	3	33	theme	phylogenetic	554:565	arg1	tree					567:570	the phylogenetic tree	550:570	the phylogenetic tree	550:570	The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT.
34705623	10	34	theme	novel	1538:1542	arg1	analyses					1526:1533	the phenotypic, genotypic and phylogenetic analyses	1483:1533	the phenotypic, genotypic and phylogenetic analyses	1483:1533	Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, a novel species Actinomadura soli sp.
34705623	10	34	theme	novel	1538:1542	arg1	species					1544:1550	a novel species Actinomadura soli sp	1536:1571	a novel species Actinomadura soli sp	1536:1571	Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, a novel species Actinomadura soli sp.
34705623	5	35	theme	genus	1075:1079	arg1	Actinomadura					1081:1092	the genus Actinomadura	1071:1092	the genus Actinomadura	1071:1092	However, the digital DNA-DNA hybridization and average nucleotide identity values between strain 14C53T with Actinomadura geliboluensis A8036T and Actinomadura pelletieri DSM 43383T were 28.6-30.2 % and 84.3-85.5 %, respectively, and comparative analyses based on the genome sequences demonstrated that it represents a novel species of the genus Actinomadura.
34705623	2	36	attach	isolated	136:143	arg1	sample					157:162	a soil sample	150:162	a soil sample on basaltic material from Samsun, Turkey	150:203	An actinobacterium, designated 14C53T, was isolated from a soil sample on basaltic material from Samsun, Turkey.
34705623	2	36	attach	isolated	136:143	arg2	actinobacterium					96:110	An actinobacterium	93:110	An actinobacterium	93:110	An actinobacterium, designated 14C53T, was isolated from a soil sample on basaltic material from Samsun, Turkey.
34705623	8	37	theme	Strain	1292:1297	arg1	14C53T					1299:1304	Strain 14C53T	1292:1304	Strain 14C53T	1292:1304	Strain 14C53T contained meso-diaminopimelic acid as the diamino acid in the cell-wall peptidoglycan.
34705623	3	38	from	°C.	373:375	arg1	optimum					359:365	an optimum	356:365	an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT	356:627	The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT.
34705623	3	39	theme	rRNA	406:409	arg1	sequences					416:424	16S rRNA gene sequences	402:424	16S rRNA gene sequences	402:424	The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT.
34705623	6	40	theme	14C53T	1121:1126	arg1	size					1106:1109	The genome size	1095:1109	The genome size of strain 14C53T	1095:1126	The genome size of strain 14C53T was approximately 9.0 Mb and the genomic DNA G+C content of the strain was 71.3 mol%.
34705623	6	40	theme	14C53T	1121:1126	arg1	9.0 Mb					1146:1151	9.0 Mb	1146:1151	9.0 Mb	1146:1151	The genome size of strain 14C53T was approximately 9.0 Mb and the genomic DNA G+C content of the strain was 71.3 mol%.
34705623	5	41	theme	 and	933:936	arg1	%					948:948	28.6-30.2 % and 84.3-85.5 %	922:948	28.6-30.2 % and 84.3-85.5 %	922:948	However, the digital DNA-DNA hybridization and average nucleotide identity values between strain 14C53T with Actinomadura geliboluensis A8036T and Actinomadura pelletieri DSM 43383T were 28.6-30.2 % and 84.3-85.5 %, respectively, and comparative analyses based on the genome sequences demonstrated that it represents a novel species of the genus Actinomadura.
34705623	5	41	theme	 and	933:936	arg1	values					810:815	the digital DNA-DNA hybridization and average nucleotide identity values	744:815	the digital DNA-DNA hybridization and average nucleotide identity values between strain 14C53T with Actinomadura geliboluensis A8036T and Actinomadura pelletieri DSM 43383T	744:915	However, the digital DNA-DNA hybridization and average nucleotide identity values between strain 14C53T with Actinomadura geliboluensis A8036T and Actinomadura pelletieri DSM 43383T were 28.6-30.2 % and 84.3-85.5 %, respectively, and comparative analyses based on the genome sequences demonstrated that it represents a novel species of the genus Actinomadura.
34705623	2	42	theme	soil	152:155	arg1	sample					157:162	a soil sample	150:162	a soil sample on basaltic material from Samsun, Turkey	150:203	An actinobacterium, designated 14C53T, was isolated from a soil sample on basaltic material from Samsun, Turkey.
34705623	4	43	theme	pelletieri	712:721	arg1	43383T					727:732	the strain and Actinomadura pelletieri DSM 43383T	684:732	43383T	727:732	The genome tree revealed a close relationship between the strain and Actinomadura pelletieri DSM 43383T.
34705623	3	44	theme	Actinomadura	596:607	arg1	D310AT					622:627	Actinomadura alkaliterrae D310AT	596:627	Actinomadura alkaliterrae D310AT	596:627	The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT.
34705623	3	45	theme	sequences	416:424	arg1	analysis					390:397	Phylogenetic analysis	377:397	Phylogenetic analysis of 16S rRNA gene sequences	377:424	The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT.
34705623	1	46	from	layer	55:59	arg1	material					73:80	basaltic material	64:80	basaltic material in Turkey	64:90	nov., isolated from the top soil layer on basaltic material in Turkey.
34705623	5	47	theme	hybridization	764:776	arg1	%					948:948	28.6-30.2 % and 84.3-85.5 %	922:948	28.6-30.2 % and 84.3-85.5 %	922:948	However, the digital DNA-DNA hybridization and average nucleotide identity values between strain 14C53T with Actinomadura geliboluensis A8036T and Actinomadura pelletieri DSM 43383T were 28.6-30.2 % and 84.3-85.5 %, respectively, and comparative analyses based on the genome sequences demonstrated that it represents a novel species of the genus Actinomadura.
34705623	5	47	theme	hybridization	764:776	arg1	values					810:815	the digital DNA-DNA hybridization and average nucleotide identity values	744:815	the digital DNA-DNA hybridization and average nucleotide identity values between strain 14C53T with Actinomadura geliboluensis A8036T and Actinomadura pelletieri DSM 43383T	744:915	However, the digital DNA-DNA hybridization and average nucleotide identity values between strain 14C53T with Actinomadura geliboluensis A8036T and Actinomadura pelletieri DSM 43383T were 28.6-30.2 % and 84.3-85.5 %, respectively, and comparative analyses based on the genome sequences demonstrated that it represents a novel species of the genus Actinomadura.
34705623	3	48	theme	growth	210:215	arg1	ranges					217:222	The growth ranges	206:222	The growth ranges for NaCl concentration and pH of strain 14C53T	206:269	The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT.
34705623	6	49	theme	genome	1099:1104	arg1	size					1106:1109	The genome size	1095:1109	The genome size of strain 14C53T	1095:1126	The genome size of strain 14C53T was approximately 9.0 Mb and the genomic DNA G+C content of the strain was 71.3 mol%.
34705623	6	49	theme	genome	1099:1104	arg1	9.0 Mb					1146:1151	9.0 Mb	1146:1151	9.0 Mb	1146:1151	The genome size of strain 14C53T was approximately 9.0 Mb and the genomic DNA G+C content of the strain was 71.3 mol%.
34705623	5	50	theme	average	782:788	arg1	identity					801:808	average nucleotide identity	782:808	average nucleotide identity	782:808	However, the digital DNA-DNA hybridization and average nucleotide identity values between strain 14C53T with Actinomadura geliboluensis A8036T and Actinomadura pelletieri DSM 43383T were 28.6-30.2 % and 84.3-85.5 %, respectively, and comparative analyses based on the genome sequences demonstrated that it represents a novel species of the genus Actinomadura.
34705623	10	51	theme	genotypic	1499:1507	arg1	analyses					1526:1533	the phenotypic, genotypic and phylogenetic analyses	1483:1533	the phenotypic, genotypic and phylogenetic analyses	1483:1533	Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, a novel species Actinomadura soli sp.
34705623	10	51	theme	genotypic	1499:1507	arg1	species					1544:1550	a novel species Actinomadura soli sp	1536:1571	a novel species Actinomadura soli sp	1536:1571	Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, a novel species Actinomadura soli sp.
34705623	5	52	theme	genome	1003:1008	arg1	sequences					1010:1018	the genome sequences	999:1018	the genome sequences	999:1018	However, the digital DNA-DNA hybridization and average nucleotide identity values between strain 14C53T with Actinomadura geliboluensis A8036T and Actinomadura pelletieri DSM 43383T were 28.6-30.2 % and 84.3-85.5 %, respectively, and comparative analyses based on the genome sequences demonstrated that it represents a novel species of the genus Actinomadura.
34705623	3	53	theme	98.5 	517:521	arg1	value					535:539	98.5 % similarity value	517:539	98.5 % similarity value	517:539	The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT.
34705623	3	53	theme	98.5 	517:521	arg1	A8036T					509:514	A8036T	509:514	A8036T	509:514	The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT.
34705623	5	54	theme	digital	748:754	arg1	hybridization					764:776	digital DNA-DNA hybridization	748:776	digital DNA-DNA hybridization	748:776	However, the digital DNA-DNA hybridization and average nucleotide identity values between strain 14C53T with Actinomadura geliboluensis A8036T and Actinomadura pelletieri DSM 43383T were 28.6-30.2 % and 84.3-85.5 %, respectively, and comparative analyses based on the genome sequences demonstrated that it represents a novel species of the genus Actinomadura.
34705623	6	55	theme	genomic	1161:1167	arg1	%					1211:1211	71.3 mol%	1203:1211	71.3 mol%	1203:1211	The genome size of strain 14C53T was approximately 9.0 Mb and the genomic DNA G+C content of the strain was 71.3 mol%.
34705623	6	55	theme	genomic	1161:1167	arg1	content					1177:1183	the genomic DNA G+C content	1157:1183	the genomic DNA G+C content of the strain	1157:1197	The genome size of strain 14C53T was approximately 9.0 Mb and the genomic DNA G+C content of the strain was 71.3 mol%.
34705623	10	56	theme	phenotypic	1487:1496	arg1	analyses					1526:1533	the phenotypic, genotypic and phylogenetic analyses	1483:1533	the phenotypic, genotypic and phylogenetic analyses	1483:1533	Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, a novel species Actinomadura soli sp.
34705623	10	56	theme	phenotypic	1487:1496	arg1	species					1544:1550	a novel species Actinomadura soli sp	1536:1571	a novel species Actinomadura soli sp	1536:1571	Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, a novel species Actinomadura soli sp.
34705623	3	57	theme	strain	257:262	arg1	14C53T					264:269	strain 14C53T	257:269	strain 14C53T	257:269	The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT.
34705623	9	58	theme	predominant	1397:1407	arg1	MK-9					1427:1430	MK-9	1427:1430	MK-9	1427:1430	The predominant menaquinones were MK-9(H8) and MK-9(H6).
34705623	9	58	theme	predominant	1397:1407	arg1	menaquinones					1409:1420	The predominant menaquinones	1393:1420	The predominant menaquinones	1393:1420	The predominant menaquinones were MK-9(H8) and MK-9(H6).
34705623	11	59	theme	104447T=KCTC	1610:1621	arg1	14C53T					1597:1602	14C53T	1597:1602	14C53T (=DSM 104447T=KCTC 39878T) as the type strain	1597:1648	nov. is proposed, with 14C53T (=DSM 104447T=KCTC 39878T) as the type strain.
34705623	11	59	theme	104447T=KCTC	1610:1621	arg1	39878T					1623:1628	=DSM 104447T=KCTC 39878T	1605:1628	=DSM 104447T=KCTC 39878T	1605:1628	nov. is proposed, with 14C53T (=DSM 104447T=KCTC 39878T) as the type strain.
34705623	8	60	from	acid	1356:1359	arg1	peptidoglycan					1378:1390	the cell-wall peptidoglycan	1364:1390	the cell-wall peptidoglycan	1364:1390	Strain 14C53T contained meso-diaminopimelic acid as the diamino acid in the cell-wall peptidoglycan.
34705623	3	61	theme	similarity	524:533	arg1	value					535:539	98.5 % similarity value	517:539	98.5 % similarity value	517:539	The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT.
34705623	3	61	theme	similarity	524:533	arg1	A8036T					509:514	A8036T	509:514	A8036T	509:514	The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT.
34705623	5	62	theme	identity	801:808	arg1	%					948:948	28.6-30.2 % and 84.3-85.5 %	922:948	28.6-30.2 % and 84.3-85.5 %	922:948	However, the digital DNA-DNA hybridization and average nucleotide identity values between strain 14C53T with Actinomadura geliboluensis A8036T and Actinomadura pelletieri DSM 43383T were 28.6-30.2 % and 84.3-85.5 %, respectively, and comparative analyses based on the genome sequences demonstrated that it represents a novel species of the genus Actinomadura.
34705623	5	62	theme	identity	801:808	arg1	values					810:815	the digital DNA-DNA hybridization and average nucleotide identity values	744:815	the digital DNA-DNA hybridization and average nucleotide identity values between strain 14C53T with Actinomadura geliboluensis A8036T and Actinomadura pelletieri DSM 43383T	744:915	However, the digital DNA-DNA hybridization and average nucleotide identity values between strain 14C53T with Actinomadura geliboluensis A8036T and Actinomadura pelletieri DSM 43383T were 28.6-30.2 % and 84.3-85.5 %, respectively, and comparative analyses based on the genome sequences demonstrated that it represents a novel species of the genus Actinomadura.
34705623	7	63	theme	14C53T	1255:1260	arg1	C16 					1267:1270	C16 	1267:1270	C16 	1267:1270	The major cellular fatty acids of strain 14C53T were C16 : 0 and iso-C16 : 0.
34705623	7	63	theme	14C53T	1255:1260	arg1	acids					1239:1243	The major cellular fatty acids	1214:1243	The major cellular fatty acids of strain 14C53T	1214:1260	The major cellular fatty acids of strain 14C53T were C16 : 0 and iso-C16 : 0.
34705623	3	64	dep	°C.	373:375	arg1	revealed					426:433	revealed	426:433	revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT	426:627	The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT.
34705623	5	65	theme	DSM	906:908	arg1	43383T					910:915	DSM 43383T	906:915	DSM 43383T	906:915	However, the digital DNA-DNA hybridization and average nucleotide identity values between strain 14C53T with Actinomadura geliboluensis A8036T and Actinomadura pelletieri DSM 43383T were 28.6-30.2 % and 84.3-85.5 %, respectively, and comparative analyses based on the genome sequences demonstrated that it represents a novel species of the genus Actinomadura.
34705623	3	66	theme	temperature	305:315	arg1	range					317:321	the growth temperature range	294:321	the growth temperature range of the strain	294:335	The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT.
34705623	3	66	theme	temperature	305:315	arg1	°C					347:348	20-37 °C	341:348	20-37 °C	341:348	The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT.
34705623	4	67	theme	close	657:661	arg1	relationship					663:674	a close relationship	655:674	a close relationship between the strain and Actinomadura pelletieri DSM 43383T	655:732	The genome tree revealed a close relationship between the strain and Actinomadura pelletieri DSM 43383T.
34705623	3	68	theme	16S	402:404	arg1	sequences					416:424	16S rRNA gene sequences	402:424	16S rRNA gene sequences	402:424	The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT.
34705623	1	69	theme	soil	50:53	arg1	layer					55:59	the top soil layer	42:59	the top soil layer on basaltic material in Turkey	42:90	nov., isolated from the top soil layer on basaltic material in Turkey.
34705623	0	70	theme	soli	13:16	arg1	sp					18:19	Actinomadura soli sp	0:19	Actinomadura soli sp.	0:20	Actinomadura soli sp.
34705623	7	71	theme	cellular	1224:1231	arg1	C16 					1267:1270	C16 	1267:1270	C16 	1267:1270	The major cellular fatty acids of strain 14C53T were C16 : 0 and iso-C16 : 0.
34705623	7	71	theme	cellular	1224:1231	arg1	acids					1239:1243	The major cellular fatty acids	1214:1243	The major cellular fatty acids of strain 14C53T	1214:1260	The major cellular fatty acids of strain 14C53T were C16 : 0 and iso-C16 : 0.
34705623	3	72	theme	strain	330:335	arg1	range					317:321	the growth temperature range	294:321	the growth temperature range of the strain	294:335	The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT.
34705623	3	72	theme	strain	330:335	arg1	°C					347:348	20-37 °C	341:348	20-37 °C	341:348	The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT.
34705623	4	73	theme	genome	634:639	arg1	tree					641:644	The genome tree	630:644	The genome tree	630:644	The genome tree revealed a close relationship between the strain and Actinomadura pelletieri DSM 43383T.
34705623	2	74	from	Turkey	198:203	arg1	material					176:183	basaltic material	167:183	basaltic material from Samsun, Turkey	167:203	An actinobacterium, designated 14C53T, was isolated from a soil sample on basaltic material from Samsun, Turkey.
34705623	8	75	contain	contained	1306:1314	arg2	acid					1336:1339	meso-diaminopimelic acid	1316:1339	meso-diaminopimelic acid	1316:1339	Strain 14C53T contained meso-diaminopimelic acid as the diamino acid in the cell-wall peptidoglycan.
34705623	8	75	contain	contained	1306:1314	arg2	acid					1356:1359	the diamino acid	1344:1359	the diamino acid in the cell-wall peptidoglycan	1344:1390	Strain 14C53T contained meso-diaminopimelic acid as the diamino acid in the cell-wall peptidoglycan.
34705623	8	75	contain	contained	1306:1314	arg1	14C53T					1299:1304	Strain 14C53T	1292:1304	Strain 14C53T	1292:1304	Strain 14C53T contained meso-diaminopimelic acid as the diamino acid in the cell-wall peptidoglycan.
34705623	4	76	theme	DSM	723:725	arg1	43383T					727:732	the strain and Actinomadura pelletieri DSM 43383T	684:732	43383T	727:732	The genome tree revealed a close relationship between the strain and Actinomadura pelletieri DSM 43383T.
34705623	5	77	theme	Actinomadura	1081:1092	arg1	species					1060:1066	a novel species	1052:1066	a novel species of the genus Actinomadura	1052:1092	However, the digital DNA-DNA hybridization and average nucleotide identity values between strain 14C53T with Actinomadura geliboluensis A8036T and Actinomadura pelletieri DSM 43383T were 28.6-30.2 % and 84.3-85.5 %, respectively, and comparative analyses based on the genome sequences demonstrated that it represents a novel species of the genus Actinomadura.
34705623	2	78	theme	basaltic	167:174	arg1	material					176:183	basaltic material	167:183	basaltic material from Samsun, Turkey	167:203	An actinobacterium, designated 14C53T, was isolated from a soil sample on basaltic material from Samsun, Turkey.
34705623	10	79	dep	species	1544:1550	arg1	sp					1570:1571	Actinomadura soli sp	1552:1571	a novel species Actinomadura soli sp	1536:1571	Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, a novel species Actinomadura soli sp.
34705623	3	80	theme	gene	411:414	arg1	sequences					416:424	16S rRNA gene sequences	402:424	16S rRNA gene sequences	402:424	The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT.
34705623	1	81	from	material	73:80	arg1	Turkey					85:90	Turkey	85:90	Turkey	85:90	nov., isolated from the top soil layer on basaltic material in Turkey.
34705623	4	82	theme	Actinomadura	699:710	arg1	43383T					727:732	the strain and Actinomadura pelletieri DSM 43383T	684:732	43383T	727:732	The genome tree revealed a close relationship between the strain and Actinomadura pelletieri DSM 43383T.
34705623	5	83	theme	%	932:932	arg1	%					948:948	28.6-30.2 % and 84.3-85.5 %	922:948	28.6-30.2 % and 84.3-85.5 %	922:948	However, the digital DNA-DNA hybridization and average nucleotide identity values between strain 14C53T with Actinomadura geliboluensis A8036T and Actinomadura pelletieri DSM 43383T were 28.6-30.2 % and 84.3-85.5 %, respectively, and comparative analyses based on the genome sequences demonstrated that it represents a novel species of the genus Actinomadura.
34705623	5	83	theme	%	932:932	arg1	values					810:815	the digital DNA-DNA hybridization and average nucleotide identity values	744:815	the digital DNA-DNA hybridization and average nucleotide identity values between strain 14C53T with Actinomadura geliboluensis A8036T and Actinomadura pelletieri DSM 43383T	744:915	However, the digital DNA-DNA hybridization and average nucleotide identity values between strain 14C53T with Actinomadura geliboluensis A8036T and Actinomadura pelletieri DSM 43383T were 28.6-30.2 % and 84.3-85.5 %, respectively, and comparative analyses based on the genome sequences demonstrated that it represents a novel species of the genus Actinomadura.
34705623	10	84	theme	soli	1565:1568	arg1	sp					1570:1571	Actinomadura soli sp	1552:1571	a novel species Actinomadura soli sp	1536:1571	Based on evidence collected from the phenotypic, genotypic and phylogenetic analyses, a novel species Actinomadura soli sp.
34705623	5	85	theme	DNA-DNA	756:762	arg1	hybridization					764:776	digital DNA-DNA hybridization	748:776	digital DNA-DNA hybridization	748:776	However, the digital DNA-DNA hybridization and average nucleotide identity values between strain 14C53T with Actinomadura geliboluensis A8036T and Actinomadura pelletieri DSM 43383T were 28.6-30.2 % and 84.3-85.5 %, respectively, and comparative analyses based on the genome sequences demonstrated that it represents a novel species of the genus Actinomadura.
34705623	8	86	theme	meso-diaminopimelic	1316:1334	arg1	acid					1356:1359	the diamino acid	1344:1359	the diamino acid in the cell-wall peptidoglycan	1344:1390	Strain 14C53T contained meso-diaminopimelic acid as the diamino acid in the cell-wall peptidoglycan.
34705623	8	86	theme	meso-diaminopimelic	1316:1334	arg1	acid					1336:1339	meso-diaminopimelic acid	1316:1339	meso-diaminopimelic acid	1316:1339	Strain 14C53T contained meso-diaminopimelic acid as the diamino acid in the cell-wall peptidoglycan.
34705623	3	87	theme	strain	440:445	arg1	14C53T					447:452	strain 14C53T	440:452	strain 14C53T	440:452	The growth ranges for NaCl concentration and pH of strain 14C53T were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53T was most closely related to Actinomadura geliboluensis A8036T (98.5 % similarity value), but in the phylogenetic tree, it formed a clade with Actinomadura alkaliterrae D310AT.
32899830	0	0	theme	Extracts	102:109	arg1	Activity					48:55	Antioxidant and Anti-Inflammatory Activity	14:55	Antioxidant and Anti-Inflammatory Activity of Anthocyanin-Rich Water-Soluble Aronia Dry Extracts	14:109	Evaluation of Antioxidant and Anti-Inflammatory Activity of Anthocyanin-Rich Water-Soluble Aronia Dry Extracts.
32899830	10	1	theme	lipid	1436:1440	arg1	peroxidation					1442:1453	lipid peroxidation	1436:1453	lipid peroxidation (Interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and malondialdehyde (MDA))	1436:1546	It was revealed that ADE standardized to 25% of anthocyanins depresses the level of markers of inflammation and lipid peroxidation (Interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and malondialdehyde (MDA)) in in vitro conditions.
32899830	10	2	theme	factor	1499:1504	arg1	TNF-α					1513:1517	TNF-α	1513:1517	TNF-α	1513:1517	It was revealed that ADE standardized to 25% of anthocyanins depresses the level of markers of inflammation and lipid peroxidation (Interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and malondialdehyde (MDA)) in in vitro conditions.
32899830	10	2	theme	factor	1499:1504	arg1	alpha					1506:1510	tumor necrosis factor alpha	1484:1510	tumor necrosis factor alpha (TNF-α)	1484:1518	It was revealed that ADE standardized to 25% of anthocyanins depresses the level of markers of inflammation and lipid peroxidation (Interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and malondialdehyde (MDA)) in in vitro conditions.
32899830	6	3	theme	higher	769:774	arg1	content					756:762	anthocyanin content	744:762	anthocyanin content much higher than the typical market standards	744:808	The high-quality chokeberry pomace enabled obtaining extracts with anthocyanin content much higher than the typical market standards.
32899830	10	4	dep	peroxidation	1442:1453	arg1	beta					1470:1473	Interleukin 1 beta	1456:1473	Interleukin 1 beta (IL-1β)	1456:1481	It was revealed that ADE standardized to 25% of anthocyanins depresses the level of markers of inflammation and lipid peroxidation (Interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and malondialdehyde (MDA)) in in vitro conditions.
32899830	10	4	dep	peroxidation	1442:1453	arg1	malondialdehyde					1525:1539	malondialdehyde	1525:1539	malondialdehyde (MDA)	1525:1545	It was revealed that ADE standardized to 25% of anthocyanins depresses the level of markers of inflammation and lipid peroxidation (Interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and malondialdehyde (MDA)) in in vitro conditions.
32899830	10	4	dep	peroxidation	1442:1453	arg1	IL-1β					1476:1480	IL-1β	1476:1480	IL-1β	1476:1480	It was revealed that ADE standardized to 25% of anthocyanins depresses the level of markers of inflammation and lipid peroxidation (Interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and malondialdehyde (MDA)) in in vitro conditions.
32899830	10	4	dep	peroxidation	1442:1453	arg1	alpha					1506:1510	tumor necrosis factor alpha	1484:1510	tumor necrosis factor alpha (TNF-α)	1484:1518	It was revealed that ADE standardized to 25% of anthocyanins depresses the level of markers of inflammation and lipid peroxidation (Interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and malondialdehyde (MDA)) in in vitro conditions.
32899830	10	4	dep	peroxidation	1442:1453	arg1	TNF-α					1513:1517	TNF-α	1513:1517	TNF-α	1513:1517	It was revealed that ADE standardized to 25% of anthocyanins depresses the level of markers of inflammation and lipid peroxidation (Interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and malondialdehyde (MDA)) in in vitro conditions.
32899830	10	4	dep	peroxidation	1442:1453	arg1	MDA					1542:1544	MDA	1542:1544	MDA	1542:1544	It was revealed that ADE standardized to 25% of anthocyanins depresses the level of markers of inflammation and lipid peroxidation (Interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and malondialdehyde (MDA)) in in vitro conditions.
32899830	9	5	theme	peroxidation	1188:1199	arg1	level					1209:1213	the level	1205:1213	the level of inflammatory markers	1205:1237	The inhibition of lipid peroxidation and the level of inflammatory markers have been also investigated using lipopolysaccharide (LPS)-stimulated RAW 264 cells.
32899830	9	5	theme	peroxidation	1188:1199	arg1	inhibition					1168:1177	The inhibition	1164:1177	The inhibition of lipid peroxidation	1164:1199	The inhibition of lipid peroxidation and the level of inflammatory markers have been also investigated using lipopolysaccharide (LPS)-stimulated RAW 264 cells.
32899830	0	6	theme	Dry	98:100	arg1	Extracts					102:109	Anthocyanin-Rich Water-Soluble Aronia Dry Extracts	60:109	Anthocyanin-Rich Water-Soluble Aronia Dry Extracts	60:109	Evaluation of Antioxidant and Anti-Inflammatory Activity of Anthocyanin-Rich Water-Soluble Aronia Dry Extracts.
32899830	8	7	theme	test	1106:1109	arg1	assay					1157:1161	Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test and Oxygen Radical Absorbance Capacity (ORAC) assay	1044:1161	Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test and Oxygen Radical Absorbance Capacity (ORAC) assay	1044:1161	Antioxidant and free-radical scavenging properties were evaluated using a 2,2'-diphenyl-1-picrylhydrazyl using Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test and Oxygen Radical Absorbance Capacity (ORAC) assay.
32899830	1	8	theme	human	182:186	arg1	health					188:193	human health	182:193	human health	182:193	Aronia fruits contain many valuable components that are beneficial to human health.
32899830	7	9	theme	fruit	913:917	arg1	preparations					919:930	other fruit preparations	907:930	other fruit preparations	907:930	Moreover, it was found that the antioxidant capacity of aronia extracts exceeded those found in other fruit preparations.
32899830	10	10	theme	inflammation	1419:1430	arg1	markers					1408:1414	markers	1408:1414	markers of inflammation and lipid peroxidation (Interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and malondialdehyde (MDA))	1408:1546	It was revealed that ADE standardized to 25% of anthocyanins depresses the level of markers of inflammation and lipid peroxidation (Interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and malondialdehyde (MDA)) in in vitro conditions.
32899830	8	11	theme	spectroscopy	1082:1093	arg1	test					1106:1109	Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test	1044:1109	Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test	1044:1109	Antioxidant and free-radical scavenging properties were evaluated using a 2,2'-diphenyl-1-picrylhydrazyl using Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test and Oxygen Radical Absorbance Capacity (ORAC) assay.
32899830	8	12	theme	Radical	1122:1128	arg1	Capacity					1141:1148	Oxygen Radical Absorbance Capacity	1115:1148	Oxygen Radical Absorbance Capacity (ORAC)	1115:1155	Antioxidant and free-radical scavenging properties were evaluated using a 2,2'-diphenyl-1-picrylhydrazyl using Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test and Oxygen Radical Absorbance Capacity (ORAC) assay.
32899830	8	12	theme	Radical	1122:1128	arg1	ORAC					1151:1154	ORAC	1151:1154	ORAC	1151:1154	Antioxidant and free-radical scavenging properties were evaluated using a 2,2'-diphenyl-1-picrylhydrazyl using Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test and Oxygen Radical Absorbance Capacity (ORAC) assay.
32899830	5	13	theme	phenolic	629:636	arg1	acids					638:642	phenolic acids	629:642	phenolic acids	629:642	Subsequently, the content of anthocyanins, phenolic acids, and polyphenols was determined.
32899830	2	14	theme	harvesting	320:329	arg1	period					331:336	harvesting period	320:336	harvesting period	320:336	However, fruits are characterized by significant variations in chemical composition dependent on the growing conditions and harvesting period.
32899830	8	15	theme	Electron	1044:1051	arg1	DPPH-EPR					1096:1103	DPPH-EPR	1096:1103	DPPH-EPR	1096:1103	Antioxidant and free-radical scavenging properties were evaluated using a 2,2'-diphenyl-1-picrylhydrazyl using Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test and Oxygen Radical Absorbance Capacity (ORAC) assay.
32899830	8	15	theme	Electron	1044:1051	arg1	spectroscopy					1082:1093	Electron Paramagnetic Resonance (EPR) spectroscopy	1044:1093	Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test	1044:1109	Antioxidant and free-radical scavenging properties were evaluated using a 2,2'-diphenyl-1-picrylhydrazyl using Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test and Oxygen Radical Absorbance Capacity (ORAC) assay.
32899830	9	16	theme	inflammatory	1218:1229	arg1	markers					1231:1237	inflammatory markers	1218:1237	inflammatory markers	1218:1237	The inhibition of lipid peroxidation and the level of inflammatory markers have been also investigated using lipopolysaccharide (LPS)-stimulated RAW 264 cells.
32899830	8	17	theme	Oxygen	1115:1120	arg1	Capacity					1141:1148	Oxygen Radical Absorbance Capacity	1115:1148	Oxygen Radical Absorbance Capacity (ORAC)	1115:1155	Antioxidant and free-radical scavenging properties were evaluated using a 2,2'-diphenyl-1-picrylhydrazyl using Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test and Oxygen Radical Absorbance Capacity (ORAC) assay.
32899830	8	17	theme	Oxygen	1115:1120	arg1	ORAC					1151:1154	ORAC	1151:1154	ORAC	1151:1154	Antioxidant and free-radical scavenging properties were evaluated using a 2,2'-diphenyl-1-picrylhydrazyl using Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test and Oxygen Radical Absorbance Capacity (ORAC) assay.
32899830	5	18	theme	acids	638:642	arg1	content					604:610	the content	600:610	the content of anthocyanins, phenolic acids, and polyphenols	600:659	Subsequently, the content of anthocyanins, phenolic acids, and polyphenols was determined.
32899830	2	19	from	variations	245:254	arg1	composition					268:278	chemical composition	259:278	chemical composition dependent on the growing conditions and harvesting period	259:336	However, fruits are characterized by significant variations in chemical composition dependent on the growing conditions and harvesting period.
32899830	11	20	theme	cytotoxic	1660:1668	arg1	effect					1670:1675	any cytotoxic effect	1656:1675	any cytotoxic effect	1656:1675	Additionally, it was confirmed that ADE at all analyzed concentrations did not show any cytotoxic effect as demonstrated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
32899830	8	21	theme	Capacity	1141:1148	arg1	assay					1157:1161	Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test and Oxygen Radical Absorbance Capacity (ORAC) assay	1044:1161	Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test and Oxygen Radical Absorbance Capacity (ORAC) assay	1044:1161	Antioxidant and free-radical scavenging properties were evaluated using a 2,2'-diphenyl-1-picrylhydrazyl using Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test and Oxygen Radical Absorbance Capacity (ORAC) assay.
32899830	8	22	theme	EPR	1077:1079	arg1	DPPH-EPR					1096:1103	DPPH-EPR	1096:1103	DPPH-EPR	1096:1103	Antioxidant and free-radical scavenging properties were evaluated using a 2,2'-diphenyl-1-picrylhydrazyl using Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test and Oxygen Radical Absorbance Capacity (ORAC) assay.
32899830	8	22	theme	EPR	1077:1079	arg1	spectroscopy					1082:1093	Electron Paramagnetic Resonance (EPR) spectroscopy	1044:1093	Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test	1044:1109	Antioxidant and free-radical scavenging properties were evaluated using a 2,2'-diphenyl-1-picrylhydrazyl using Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test and Oxygen Radical Absorbance Capacity (ORAC) assay.
32899830	7	23	theme	other	907:911	arg1	preparations					919:930	other fruit preparations	907:930	other fruit preparations	907:930	Moreover, it was found that the antioxidant capacity of aronia extracts exceeded those found in other fruit preparations.
32899830	3	24	theme	health-promoting	428:443	arg1	substances					445:454	health-promoting substances	428:454	health-promoting substances	428:454	Therefore, there is a need to formulate the extracts with a precisely defined content of health-promoting substances.
32899830	2	25	theme	growing	297:303	arg1	conditions					305:314	the growing conditions	293:314	the growing conditions	293:314	However, fruits are characterized by significant variations in chemical composition dependent on the growing conditions and harvesting period.
32899830	6	26	theme	anthocyanin	744:754	arg1	content					756:762	anthocyanin content	744:762	anthocyanin content much higher than the typical market standards	744:808	The high-quality chokeberry pomace enabled obtaining extracts with anthocyanin content much higher than the typical market standards.
32899830	4	27	theme	frozen	502:507	arg1	pomace					509:514	frozen pomace	502:514	frozen pomace applying water extraction, followed by purification and spray-drying	502:583	Aronia dry extracts (ADE) were prepared from frozen pomace applying water extraction, followed by purification and spray-drying.
32899830	5	28	theme	polyphenols	649:659	arg1	content					604:610	the content	600:610	the content of anthocyanins, phenolic acids, and polyphenols	600:659	Subsequently, the content of anthocyanins, phenolic acids, and polyphenols was determined.
32899830	11	29	from	concentrations	1628:1641	arg1	ADE					1608:1610	ADE	1608:1610	ADE at all analyzed concentrations	1608:1641	Additionally, it was confirmed that ADE at all analyzed concentrations did not show any cytotoxic effect as demonstrated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
32899830	3	30	theme	defined	409:415	arg1	content					417:423	a precisely defined content	397:423	a precisely defined content of health-promoting substances	397:454	Therefore, there is a need to formulate the extracts with a precisely defined content of health-promoting substances.
32899830	7	31	theme	antioxidant	843:853	arg1	capacity					855:862	the antioxidant capacity	839:862	the antioxidant capacity of aronia extracts	839:881	Moreover, it was found that the antioxidant capacity of aronia extracts exceeded those found in other fruit preparations.
32899830	11	32	theme	bromide	1749:1755	arg1	assay					1763:1767	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay	1696:1767	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay	1696:1767	Additionally, it was confirmed that ADE at all analyzed concentrations did not show any cytotoxic effect as demonstrated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
32899830	8	33	theme	Resonance	1066:1074	arg1	DPPH-EPR					1096:1103	DPPH-EPR	1096:1103	DPPH-EPR	1096:1103	Antioxidant and free-radical scavenging properties were evaluated using a 2,2'-diphenyl-1-picrylhydrazyl using Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test and Oxygen Radical Absorbance Capacity (ORAC) assay.
32899830	8	33	theme	Resonance	1066:1074	arg1	spectroscopy					1082:1093	Electron Paramagnetic Resonance (EPR) spectroscopy	1044:1093	Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test	1044:1109	Antioxidant and free-radical scavenging properties were evaluated using a 2,2'-diphenyl-1-picrylhydrazyl using Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test and Oxygen Radical Absorbance Capacity (ORAC) assay.
32899830	1	34	theme	Aronia	112:117	arg1	fruits					119:124	Aronia fruits	112:124	Aronia fruits	112:124	Aronia fruits contain many valuable components that are beneficial to human health.
32899830	7	35	theme	extracts	874:881	arg1	capacity					855:862	the antioxidant capacity	839:862	the antioxidant capacity of aronia extracts	839:881	Moreover, it was found that the antioxidant capacity of aronia extracts exceeded those found in other fruit preparations.
32899830	10	36	theme	necrosis	1490:1497	arg1	TNF-α					1513:1517	TNF-α	1513:1517	TNF-α	1513:1517	It was revealed that ADE standardized to 25% of anthocyanins depresses the level of markers of inflammation and lipid peroxidation (Interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and malondialdehyde (MDA)) in in vitro conditions.
32899830	10	36	theme	necrosis	1490:1497	arg1	alpha					1506:1510	tumor necrosis factor alpha	1484:1510	tumor necrosis factor alpha (TNF-α)	1484:1518	It was revealed that ADE standardized to 25% of anthocyanins depresses the level of markers of inflammation and lipid peroxidation (Interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and malondialdehyde (MDA)) in in vitro conditions.
32899830	10	37	theme	tumor	1484:1488	arg1	TNF-α					1513:1517	TNF-α	1513:1517	TNF-α	1513:1517	It was revealed that ADE standardized to 25% of anthocyanins depresses the level of markers of inflammation and lipid peroxidation (Interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and malondialdehyde (MDA)) in in vitro conditions.
32899830	10	37	theme	tumor	1484:1488	arg1	alpha					1506:1510	tumor necrosis factor alpha	1484:1510	tumor necrosis factor alpha (TNF-α)	1484:1518	It was revealed that ADE standardized to 25% of anthocyanins depresses the level of markers of inflammation and lipid peroxidation (Interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and malondialdehyde (MDA)) in in vitro conditions.
32899830	5	38	theme	anthocyanins	615:626	arg1	content					604:610	the content	600:610	the content of anthocyanins, phenolic acids, and polyphenols	600:659	Subsequently, the content of anthocyanins, phenolic acids, and polyphenols was determined.
32899830	10	39	theme	anthocyanins	1372:1383	arg1	anthocyanins					1372:1383	anthocyanins	1372:1383	anthocyanins	1372:1383	It was revealed that ADE standardized to 25% of anthocyanins depresses the level of markers of inflammation and lipid peroxidation (Interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and malondialdehyde (MDA)) in in vitro conditions.
32899830	10	39	theme	anthocyanins	1372:1383	arg1	%					1367:1367	25%	1365:1367	25% of anthocyanins	1365:1383	It was revealed that ADE standardized to 25% of anthocyanins depresses the level of markers of inflammation and lipid peroxidation (Interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and malondialdehyde (MDA)) in in vitro conditions.
32899830	11	40	theme	analyzed	1619:1626	arg1	concentrations					1628:1641	all analyzed concentrations	1615:1641	all analyzed concentrations	1615:1641	Additionally, it was confirmed that ADE at all analyzed concentrations did not show any cytotoxic effect as demonstrated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
32899830	0	41	theme	Antioxidant	14:24	arg1	Activity					48:55	Antioxidant and Anti-Inflammatory Activity	14:55	Antioxidant and Anti-Inflammatory Activity of Anthocyanin-Rich Water-Soluble Aronia Dry Extracts	14:109	Evaluation of Antioxidant and Anti-Inflammatory Activity of Anthocyanin-Rich Water-Soluble Aronia Dry Extracts.
32899830	9	42	theme	RAW	1309:1311	arg1	cells					1317:1321	lipopolysaccharide (LPS)-stimulated RAW 264 cells	1273:1321	lipopolysaccharide (LPS)-stimulated RAW 264 cells	1273:1321	The inhibition of lipid peroxidation and the level of inflammatory markers have been also investigated using lipopolysaccharide (LPS)-stimulated RAW 264 cells.
32899830	8	43	theme	scavenging	962:971	arg1	properties					973:982	Antioxidant and free-radical scavenging properties	933:982	Antioxidant and free-radical scavenging properties	933:982	Antioxidant and free-radical scavenging properties were evaluated using a 2,2'-diphenyl-1-picrylhydrazyl using Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test and Oxygen Radical Absorbance Capacity (ORAC) assay.
32899830	9	44	theme	lipid	1182:1186	arg1	peroxidation					1188:1199	lipid peroxidation	1182:1199	lipid peroxidation	1182:1199	The inhibition of lipid peroxidation and the level of inflammatory markers have been also investigated using lipopolysaccharide (LPS)-stimulated RAW 264 cells.
32899830	8	45	theme	free-radical	949:960	arg1	properties					973:982	Antioxidant and free-radical scavenging properties	933:982	Antioxidant and free-radical scavenging properties	933:982	Antioxidant and free-radical scavenging properties were evaluated using a 2,2'-diphenyl-1-picrylhydrazyl using Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test and Oxygen Radical Absorbance Capacity (ORAC) assay.
32899830	0	46	theme	Activity	48:55	arg1	Evaluation					0:9	Evaluation	0:9	Evaluation of Antioxidant and Anti-Inflammatory Activity of Anthocyanin-Rich Water-Soluble Aronia Dry Extracts.	0:110	Evaluation of Antioxidant and Anti-Inflammatory Activity of Anthocyanin-Rich Water-Soluble Aronia Dry Extracts.
32899830	2	47	theme	chemical	259:266	arg1	composition					268:278	chemical composition	259:278	chemical composition dependent on the growing conditions and harvesting period	259:336	However, fruits are characterized by significant variations in chemical composition dependent on the growing conditions and harvesting period.
32899830	7	48	theme	aronia	867:872	arg1	extracts					874:881	aronia extracts	867:881	aronia extracts	867:881	Moreover, it was found that the antioxidant capacity of aronia extracts exceeded those found in other fruit preparations.
32899830	0	49	theme	Anti-Inflammatory	30:46	arg1	Activity					48:55	Antioxidant and Anti-Inflammatory Activity	14:55	Antioxidant and Anti-Inflammatory Activity of Anthocyanin-Rich Water-Soluble Aronia Dry Extracts	14:109	Evaluation of Antioxidant and Anti-Inflammatory Activity of Anthocyanin-Rich Water-Soluble Aronia Dry Extracts.
32899830	1	50	contain	contain	126:132	arg1	fruits					119:124	Aronia fruits	112:124	Aronia fruits	112:124	Aronia fruits contain many valuable components that are beneficial to human health.
32899830	1	50	contain	contain	126:132	arg2	components					148:157	many valuable components	134:157	many valuable components that are beneficial to human health	134:193	Aronia fruits contain many valuable components that are beneficial to human health.
32899830	3	51	theme	substances	445:454	arg1	content					417:423	a precisely defined content	397:423	a precisely defined content of health-promoting substances	397:454	Therefore, there is a need to formulate the extracts with a precisely defined content of health-promoting substances.
32899830	8	52	theme	Absorbance	1130:1139	arg1	Capacity					1141:1148	Oxygen Radical Absorbance Capacity	1115:1148	Oxygen Radical Absorbance Capacity (ORAC)	1115:1155	Antioxidant and free-radical scavenging properties were evaluated using a 2,2'-diphenyl-1-picrylhydrazyl using Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test and Oxygen Radical Absorbance Capacity (ORAC) assay.
32899830	8	52	theme	Absorbance	1130:1139	arg1	ORAC					1151:1154	ORAC	1151:1154	ORAC	1151:1154	Antioxidant and free-radical scavenging properties were evaluated using a 2,2'-diphenyl-1-picrylhydrazyl using Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test and Oxygen Radical Absorbance Capacity (ORAC) assay.
32899830	0	53	theme	Anthocyanin-Rich	60:75	arg1	Extracts					102:109	Anthocyanin-Rich Water-Soluble Aronia Dry Extracts	60:109	Anthocyanin-Rich Water-Soluble Aronia Dry Extracts	60:109	Evaluation of Antioxidant and Anti-Inflammatory Activity of Anthocyanin-Rich Water-Soluble Aronia Dry Extracts.
32899830	7	54	located	found	898:902	arg1	preparations					919:930	other fruit preparations	907:930	other fruit preparations	907:930	Moreover, it was found that the antioxidant capacity of aronia extracts exceeded those found in other fruit preparations.
32899830	7	54	located	found	898:902	arg2	those					892:896	those	892:896	those	892:896	Moreover, it was found that the antioxidant capacity of aronia extracts exceeded those found in other fruit preparations.
32899830	1	55	theme	many	134:137	arg1	components					148:157	many valuable components	134:157	many valuable components that are beneficial to human health	134:193	Aronia fruits contain many valuable components that are beneficial to human health.
32899830	10	56	theme	Interleukin	1456:1466	arg1	IL-1β					1476:1480	IL-1β	1476:1480	IL-1β	1476:1480	It was revealed that ADE standardized to 25% of anthocyanins depresses the level of markers of inflammation and lipid peroxidation (Interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and malondialdehyde (MDA)) in in vitro conditions.
32899830	10	56	theme	Interleukin	1456:1466	arg1	beta					1470:1473	Interleukin 1 beta	1456:1473	Interleukin 1 beta (IL-1β)	1456:1481	It was revealed that ADE standardized to 25% of anthocyanins depresses the level of markers of inflammation and lipid peroxidation (Interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and malondialdehyde (MDA)) in in vitro conditions.
32899830	10	57	dep	in	1551:1552	arg1	vitro					1554:1558	vitro	1554:1558	vitro	1554:1558	It was revealed that ADE standardized to 25% of anthocyanins depresses the level of markers of inflammation and lipid peroxidation (Interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and malondialdehyde (MDA)) in in vitro conditions.
32899830	4	58	theme	Aronia	457:462	arg1	ADE					478:480	ADE	478:480	ADE	478:480	Aronia dry extracts (ADE) were prepared from frozen pomace applying water extraction, followed by purification and spray-drying.
32899830	4	58	theme	Aronia	457:462	arg1	extracts					468:475	Aronia dry extracts	457:475	Aronia dry extracts (ADE)	457:481	Aronia dry extracts (ADE) were prepared from frozen pomace applying water extraction, followed by purification and spray-drying.
32899830	2	59	theme	significant	233:243	arg1	variations					245:254	significant variations	233:254	significant variations in chemical composition dependent on the growing conditions and harvesting period	233:336	However, fruits are characterized by significant variations in chemical composition dependent on the growing conditions and harvesting period.
32899830	1	60	theme	valuable	139:146	arg1	components					148:157	many valuable components	134:157	many valuable components that are beneficial to human health	134:193	Aronia fruits contain many valuable components that are beneficial to human health.
32899830	6	61	theme	chokeberry	694:703	arg1	pomace					705:710	The high-quality chokeberry pomace	677:710	The high-quality chokeberry pomace	677:710	The high-quality chokeberry pomace enabled obtaining extracts with anthocyanin content much higher than the typical market standards.
32899830	9	62	theme	-stimulated	1297:1307	arg1	cells					1317:1321	lipopolysaccharide (LPS)-stimulated RAW 264 cells	1273:1321	lipopolysaccharide (LPS)-stimulated RAW 264 cells	1273:1321	The inhibition of lipid peroxidation and the level of inflammatory markers have been also investigated using lipopolysaccharide (LPS)-stimulated RAW 264 cells.
32899830	6	63	with	extracts	730:737	arg1	content					756:762	anthocyanin content	744:762	anthocyanin content much higher than the typical market standards	744:808	The high-quality chokeberry pomace enabled obtaining extracts with anthocyanin content much higher than the typical market standards.
32899830	6	64	theme	market	793:798	arg1	standards					800:808	the typical market standards	781:808	the typical market standards	781:808	The high-quality chokeberry pomace enabled obtaining extracts with anthocyanin content much higher than the typical market standards.
32899830	6	65	theme	high-quality	681:692	arg1	pomace					705:710	The high-quality chokeberry pomace	677:710	The high-quality chokeberry pomace	677:710	The high-quality chokeberry pomace enabled obtaining extracts with anthocyanin content much higher than the typical market standards.
32899830	11	66	theme	-2,5-diphenyltetrazolium	1724:1747	arg1	assay					1763:1767	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay	1696:1767	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay	1696:1767	Additionally, it was confirmed that ADE at all analyzed concentrations did not show any cytotoxic effect as demonstrated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
32899830	10	67	theme	markers	1408:1414	arg1	level					1399:1403	the level	1395:1403	the level of markers of inflammation and lipid peroxidation (Interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and malondialdehyde (MDA))	1395:1546	It was revealed that ADE standardized to 25% of anthocyanins depresses the level of markers of inflammation and lipid peroxidation (Interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and malondialdehyde (MDA)) in in vitro conditions.
32899830	6	68	theme	typical	785:791	arg1	standards					800:808	the typical market standards	781:808	the typical market standards	781:808	The high-quality chokeberry pomace enabled obtaining extracts with anthocyanin content much higher than the typical market standards.
32899830	8	69	theme	Antioxidant	933:943	arg1	properties					973:982	Antioxidant and free-radical scavenging properties	933:982	Antioxidant and free-radical scavenging properties	933:982	Antioxidant and free-radical scavenging properties were evaluated using a 2,2'-diphenyl-1-picrylhydrazyl using Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test and Oxygen Radical Absorbance Capacity (ORAC) assay.
32899830	0	70	theme	Aronia	91:96	arg1	Extracts					102:109	Anthocyanin-Rich Water-Soluble Aronia Dry Extracts	60:109	Anthocyanin-Rich Water-Soluble Aronia Dry Extracts	60:109	Evaluation of Antioxidant and Anti-Inflammatory Activity of Anthocyanin-Rich Water-Soluble Aronia Dry Extracts.
32899830	4	71	theme	dry	464:466	arg1	ADE					478:480	ADE	478:480	ADE	478:480	Aronia dry extracts (ADE) were prepared from frozen pomace applying water extraction, followed by purification and spray-drying.
32899830	4	71	theme	dry	464:466	arg1	extracts					468:475	Aronia dry extracts	457:475	Aronia dry extracts (ADE)	457:481	Aronia dry extracts (ADE) were prepared from frozen pomace applying water extraction, followed by purification and spray-drying.
32899830	2	72	theme	dependent	280:288	arg1	composition					268:278	chemical composition	259:278	chemical composition dependent on the growing conditions and harvesting period	259:336	However, fruits are characterized by significant variations in chemical composition dependent on the growing conditions and harvesting period.
32899830	9	73	theme	markers	1231:1237	arg1	level					1209:1213	the level	1205:1213	the level of inflammatory markers	1205:1237	The inhibition of lipid peroxidation and the level of inflammatory markers have been also investigated using lipopolysaccharide (LPS)-stimulated RAW 264 cells.
32899830	9	73	theme	markers	1231:1237	arg1	inhibition					1168:1177	The inhibition	1164:1177	The inhibition of lipid peroxidation	1164:1199	The inhibition of lipid peroxidation and the level of inflammatory markers have been also investigated using lipopolysaccharide (LPS)-stimulated RAW 264 cells.
32899830	10	74	theme	in	1551:1552	arg1	conditions					1560:1569	in vitro conditions	1551:1569	in vitro conditions	1551:1569	It was revealed that ADE standardized to 25% of anthocyanins depresses the level of markers of inflammation and lipid peroxidation (Interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and malondialdehyde (MDA)) in in vitro conditions.
32899830	10	75	theme	peroxidation	1442:1453	arg1	markers					1408:1414	markers	1408:1414	markers of inflammation and lipid peroxidation (Interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and malondialdehyde (MDA))	1408:1546	It was revealed that ADE standardized to 25% of anthocyanins depresses the level of markers of inflammation and lipid peroxidation (Interleukin 1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), and malondialdehyde (MDA)) in in vitro conditions.
32899830	0	76	theme	Water-Soluble	77:89	arg1	Extracts					102:109	Anthocyanin-Rich Water-Soluble Aronia Dry Extracts	60:109	Anthocyanin-Rich Water-Soluble Aronia Dry Extracts	60:109	Evaluation of Antioxidant and Anti-Inflammatory Activity of Anthocyanin-Rich Water-Soluble Aronia Dry Extracts.
32899830	4	77	theme	water	525:529	arg1	extraction					531:540	water extraction	525:540	water extraction	525:540	Aronia dry extracts (ADE) were prepared from frozen pomace applying water extraction, followed by purification and spray-drying.
32899830	8	78	theme	Paramagnetic	1053:1064	arg1	DPPH-EPR					1096:1103	DPPH-EPR	1096:1103	DPPH-EPR	1096:1103	Antioxidant and free-radical scavenging properties were evaluated using a 2,2'-diphenyl-1-picrylhydrazyl using Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test and Oxygen Radical Absorbance Capacity (ORAC) assay.
32899830	8	78	theme	Paramagnetic	1053:1064	arg1	spectroscopy					1082:1093	Electron Paramagnetic Resonance (EPR) spectroscopy	1044:1093	Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test	1044:1109	Antioxidant and free-radical scavenging properties were evaluated using a 2,2'-diphenyl-1-picrylhydrazyl using Electron Paramagnetic Resonance (EPR) spectroscopy (DPPH-EPR) test and Oxygen Radical Absorbance Capacity (ORAC) assay.
32702056	14	0	theme	healthy	1915:1921	arg1	flour					1904:1908	breadfruit flour	1893:1908	breadfruit flour	1893:1908	No negative health outcomes were observed in studies with in vitro or in vivo models and breadfruit flour is a healthy alternative to other starches for modern foods.
32702056	14	0	theme	healthy	1915:1921	arg1	alternative					1923:1933	a healthy alternative	1913:1933	a healthy alternative to other starches for modern foods	1913:1968	No negative health outcomes were observed in studies with in vitro or in vivo models and breadfruit flour is a healthy alternative to other starches for modern foods.
32702056	10	1	theme	death	1430:1434	arg1	sign					1385:1388	No sign	1382:1388	No sign of malnutrition, discomfort, illness or death	1382:1434	No sign of malnutrition, discomfort, illness or death was observed among the mice because of the diet.
32702056	9	2	used	used	1327:1330	arg2	diets					1280:1284	The diets	1276:1284	The diets	1276:1284	The diets were isocaloric, nutrient equivalent and used to feed male and female C57BL/6 mice for 21 days.
32702056	9	2	used	used	1327:1330	arg2	equivalent					1312:1321	equivalent	1312:1321	equivalent	1312:1321	The diets were isocaloric, nutrient equivalent and used to feed male and female C57BL/6 mice for 21 days.
32702056	6	3	theme	flour	850:854	arg1	digestions					856:865	The flour digestions	846:865	The flour digestions	846:865	The flour digestions were applied to Caco-2 cells to test the cytotoxicity and to measure the immunogenicity through cytokine expression.
32702056	3	4	theme	fundamental	504:514	arg1	data					516:519	basic and fundamental data	494:519	basic and fundamental data on impacts of a breadfruit-based diet	494:557	We designed a series of studies to provide basic and fundamental data on impacts of a breadfruit-based diet through an in vitro and in vivo model.
32702056	4	5	theme	breadfruit	605:614	arg1	flour					616:620	Cooked breadfruit flour	598:620	Cooked breadfruit flour	598:620	Cooked breadfruit flour was digested through a multi-stage enzyme digestion model to estimate protein digestibility in comparison to wheat flour.
32702056	11	6	theme	ileum	1539:1543	arg1	expression					1516:1525	the cytokine expression	1503:1525	the cytokine expression of the mice ileum	1503:1543	The histology and the cytokine expression of the mice ileum from both groups were analyzed and showed similar results.
32702056	11	6	theme	ileum	1539:1543	arg1	histology					1489:1497	histology	1489:1497	histology	1489:1497	The histology and the cytokine expression of the mice ileum from both groups were analyzed and showed similar results.
32702056	2	7	theme	gluten-free	247:257	arg1	Flour					211:215	Flour	211:215	Flour produced from breadfruit	211:240	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	2	7	theme	gluten-free	247:257	arg1	option					316:321	nutrient dense and complete protein option	280:321	nutrient dense and complete protein option for modern foods	280:338	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	2	7	theme	gluten-free	247:257	arg1	index					273:277	a gluten-free, low glycemic index	245:277	index	273:277	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	9	8	theme	C57BL/6	1356:1362	arg1	mice					1364:1367	male and female C57BL/6 mice	1340:1367	male and female C57BL/6 mice	1340:1367	The diets were isocaloric, nutrient equivalent and used to feed male and female C57BL/6 mice for 21 days.
32702056	2	9	theme	nutrient	280:287	arg1	option					316:321	nutrient dense and complete protein option	280:321	nutrient dense and complete protein option for modern foods	280:338	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	2	9	theme	nutrient	280:287	arg1	index					273:277	a gluten-free, low glycemic index	245:277	index	273:277	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	1	10	theme	worldwide	164:172	arg1	security					179:186	worldwide food security	164:186	worldwide food security	164:186	Breadfruit is a traditional staple crop from Pacific islands with the potential to improve worldwide food security and mitigate diabetes.
32702056	12	11	theme	major	1622:1626	arg1	bacteria					1628:1635	major bacteria	1622:1635	major bacteria	1622:1635	The expression of major bacteria was measured in the colon and showed similar results.
32702056	14	12	dep	in	1874:1875	arg1	vivo					1877:1880	vivo	1877:1880	vivo	1877:1880	No negative health outcomes were observed in studies with in vitro or in vivo models and breadfruit flour is a healthy alternative to other starches for modern foods.
32702056	3	13	dep	in	570:571	arg1	vitro					573:577	vitro	573:577	vitro	573:577	We designed a series of studies to provide basic and fundamental data on impacts of a breadfruit-based diet through an in vitro and in vivo model.
32702056	4	14	theme	enzyme	657:662	arg1	model					674:678	a multi-stage enzyme digestion model	643:678	a multi-stage enzyme digestion model to estimate protein digestibility in comparison to wheat flour	643:741	Cooked breadfruit flour was digested through a multi-stage enzyme digestion model to estimate protein digestibility in comparison to wheat flour.
32702056	7	15	dep	cytokines	1048:1056	arg1	IFN-γ					1085:1089	IFN-γ	1085:1089	IFN-γ	1085:1089	No significant differences were observed for immune factors and cytokines (IL-4, IL-10, IL-8, TNF-α, IFN-γ) on Caco-2 cells between the breadfruit and wheat groups.
32702056	7	15	dep	cytokines	1048:1056	arg1	IL-10					1065:1069	IL-10	1065:1069	IL-10	1065:1069	No significant differences were observed for immune factors and cytokines (IL-4, IL-10, IL-8, TNF-α, IFN-γ) on Caco-2 cells between the breadfruit and wheat groups.
32702056	7	15	dep	cytokines	1048:1056	arg1	IL-4					1059:1062	IL-4	1059:1062	IL-4	1059:1062	No significant differences were observed for immune factors and cytokines (IL-4, IL-10, IL-8, TNF-α, IFN-γ) on Caco-2 cells between the breadfruit and wheat groups.
32702056	7	15	dep	cytokines	1048:1056	arg1	TNF-α					1078:1082	TNF-α	1078:1082	TNF-α	1078:1082	No significant differences were observed for immune factors and cytokines (IL-4, IL-10, IL-8, TNF-α, IFN-γ) on Caco-2 cells between the breadfruit and wheat groups.
32702056	7	15	dep	cytokines	1048:1056	arg1	IL-8					1072:1075	IL-8	1072:1075	IL-8	1072:1075	No significant differences were observed for immune factors and cytokines (IL-4, IL-10, IL-8, TNF-α, IFN-γ) on Caco-2 cells between the breadfruit and wheat groups.
32702056	5	16	theme	wheat	801:805	arg1	protein					807:813	wheat protein	801:813	wheat protein	801:813	Breadfruit protein was found to be easier to digest than wheat protein in the enzyme digestion model.
32702056	4	17	theme	digestion	664:672	arg1	model					674:678	a multi-stage enzyme digestion model	643:678	a multi-stage enzyme digestion model to estimate protein digestibility in comparison to wheat flour	643:741	Cooked breadfruit flour was digested through a multi-stage enzyme digestion model to estimate protein digestibility in comparison to wheat flour.
32702056	14	18	with	studies	1849:1855	arg1	models					1882:1887	in vitro or in vivo models	1862:1887	in vitro or in vivo models	1862:1887	No negative health outcomes were observed in studies with in vitro or in vivo models and breadfruit flour is a healthy alternative to other starches for modern foods.
32702056	3	19	theme	breadfruit-based	537:552	arg1	diet					554:557	a breadfruit-based diet	535:557	a breadfruit-based diet	535:557	We designed a series of studies to provide basic and fundamental data on impacts of a breadfruit-based diet through an in vitro and in vivo model.
32702056	1	20	theme	staple	101:106	arg1	crop					108:111	a traditional staple crop	87:111	a traditional staple crop from Pacific islands with the potential to improve worldwide food security and mitigate diabetes	87:208	Breadfruit is a traditional staple crop from Pacific islands with the potential to improve worldwide food security and mitigate diabetes.
32702056	1	20	theme	staple	101:106	arg1	Breadfruit					73:82	Breadfruit	73:82	Breadfruit	73:82	Breadfruit is a traditional staple crop from Pacific islands with the potential to improve worldwide food security and mitigate diabetes.
32702056	13	21	theme	standard	1780:1787	arg1	mice					1798:1801	standard diet fed mice	1780:1801	standard diet fed mice	1780:1801	Mice fed the breadfruit diet had a significantly higher growth rate and body weight than standard diet fed mice.
32702056	7	22	theme	significant	987:997	arg1	differences					999:1009	No significant differences	984:1009	No significant differences	984:1009	No significant differences were observed for immune factors and cytokines (IL-4, IL-10, IL-8, TNF-α, IFN-γ) on Caco-2 cells between the breadfruit and wheat groups.
32702056	13	23	theme	fed	1794:1796	arg1	mice					1798:1801	standard diet fed mice	1780:1801	standard diet fed mice	1780:1801	Mice fed the breadfruit diet had a significantly higher growth rate and body weight than standard diet fed mice.
32702056	2	24	theme	breadfruit-based	394:409	arg1	diet					411:414	a breadfruit-based diet	392:414	a breadfruit-based diet in animals and humans	392:436	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	4	25	theme	multi-stage	645:655	arg1	model					674:678	a multi-stage enzyme digestion model	643:678	a multi-stage enzyme digestion model to estimate protein digestibility in comparison to wheat flour	643:741	Cooked breadfruit flour was digested through a multi-stage enzyme digestion model to estimate protein digestibility in comparison to wheat flour.
32702056	0	26	theme	food	58:61	arg1	security					63:70	food security	58:70	food security	58:70	Breadfruit flour is a healthy option for modern foods and food security.
32702056	2	27	theme	low	260:262	arg1	Flour					211:215	Flour	211:215	Flour produced from breadfruit	211:240	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	2	27	theme	low	260:262	arg1	option					316:321	nutrient dense and complete protein option	280:321	nutrient dense and complete protein option for modern foods	280:338	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	2	27	theme	low	260:262	arg1	index					273:277	a gluten-free, low glycemic index	245:277	index	273:277	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	14	28	theme	in	1874:1875	arg1	models					1882:1887	in vitro or in vivo models	1862:1887	in vitro or in vivo models	1862:1887	No negative health outcomes were observed in studies with in vitro or in vivo models and breadfruit flour is a healthy alternative to other starches for modern foods.
32702056	14	29	theme	other	1938:1942	arg1	starches					1944:1951	other starches	1938:1951	other starches	1938:1951	No negative health outcomes were observed in studies with in vitro or in vivo models and breadfruit flour is a healthy alternative to other starches for modern foods.
32702056	4	30	theme	protein	692:698	arg1	digestibility					700:712	protein digestibility	692:712	protein digestibility	692:712	Cooked breadfruit flour was digested through a multi-stage enzyme digestion model to estimate protein digestibility in comparison to wheat flour.
32702056	13	31	theme	growth	1747:1752	arg1	rate					1754:1757	a significantly higher growth rate	1724:1757	a significantly higher growth rate	1724:1757	Mice fed the breadfruit diet had a significantly higher growth rate and body weight than standard diet fed mice.
32702056	2	32	theme	scientific	350:359	arg1	knowledge					361:369	basic scientific knowledge	344:369	basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans	344:436	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	0	33	theme	Breadfruit	0:9	arg1	option					30:35	a healthy option	20:35	a healthy option for modern foods and food security	20:70	Breadfruit flour is a healthy option for modern foods and food security.
32702056	0	33	theme	Breadfruit	0:9	arg1	flour					11:15	Breadfruit flour	0:15	Breadfruit flour	0:15	Breadfruit flour is a healthy option for modern foods and food security.
32702056	2	34	theme	diet	411:414	arg1	impacts					381:387	health impacts	374:387	health impacts of a breadfruit-based diet in animals and humans	374:436	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	13	35	theme	breadfruit	1704:1713	arg1	diet					1715:1718	the breadfruit diet	1700:1718	the breadfruit diet	1700:1718	Mice fed the breadfruit diet had a significantly higher growth rate and body weight than standard diet fed mice.
32702056	1	36	theme	Pacific	118:124	arg1	islands					126:132	Pacific islands	118:132	Pacific islands	118:132	Breadfruit is a traditional staple crop from Pacific islands with the potential to improve worldwide food security and mitigate diabetes.
32702056	7	37	located	observed	1016:1023	arg2	differences					999:1009	No significant differences	984:1009	No significant differences	984:1009	No significant differences were observed for immune factors and cytokines (IL-4, IL-10, IL-8, TNF-α, IFN-γ) on Caco-2 cells between the breadfruit and wheat groups.
32702056	7	37	located	observed	1016:1023	arg1	cells					1102:1106	Caco-2 cells	1095:1106	Caco-2 cells	1095:1106	No significant differences were observed for immune factors and cytokines (IL-4, IL-10, IL-8, TNF-α, IFN-γ) on Caco-2 cells between the breadfruit and wheat groups.
32702056	2	38	theme	impacts	381:387	arg1	knowledge					361:369	basic scientific knowledge	344:369	basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans	344:436	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	8	39	theme	rodent	1168:1173	arg1	chow					1175:1178	A breadfruit-based rodent chow	1149:1178	A breadfruit-based rodent chow	1149:1178	A breadfruit-based rodent chow was formulated by substitution of all of the wheat in the standard formulation with breadfruit.
32702056	0	40	theme	healthy	22:28	arg1	option					30:35	a healthy option	20:35	a healthy option for modern foods and food security	20:70	Breadfruit flour is a healthy option for modern foods and food security.
32702056	0	40	theme	healthy	22:28	arg1	flour					11:15	Breadfruit flour	0:15	Breadfruit flour	0:15	Breadfruit flour is a healthy option for modern foods and food security.
32702056	14	41	theme	health	1816:1821	arg1	outcomes					1823:1830	No negative health outcomes	1804:1830	No negative health outcomes	1804:1830	No negative health outcomes were observed in studies with in vitro or in vivo models and breadfruit flour is a healthy alternative to other starches for modern foods.
32702056	3	42	theme	in	570:571	arg1	model					591:595	an in vitro and in vivo model	567:595	an in vitro and in vivo model	567:595	We designed a series of studies to provide basic and fundamental data on impacts of a breadfruit-based diet through an in vitro and in vivo model.
32702056	14	43	located	observed	1837:1844	arg2	outcomes					1823:1830	No negative health outcomes	1804:1830	No negative health outcomes	1804:1830	No negative health outcomes were observed in studies with in vitro or in vivo models and breadfruit flour is a healthy alternative to other starches for modern foods.
32702056	14	43	located	observed	1837:1844	arg1	studies					1849:1855	studies	1849:1855	studies with in vitro or in vivo models	1849:1887	No negative health outcomes were observed in studies with in vitro or in vivo models and breadfruit flour is a healthy alternative to other starches for modern foods.
32702056	2	44	theme	complete	299:306	arg1	option					316:321	nutrient dense and complete protein option	280:321	nutrient dense and complete protein option for modern foods	280:338	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	2	44	theme	complete	299:306	arg1	index					273:277	a gluten-free, low glycemic index	245:277	index	273:277	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	3	45	dep	in	583:584	arg1	vivo					586:589	vivo	586:589	vivo	586:589	We designed a series of studies to provide basic and fundamental data on impacts of a breadfruit-based diet through an in vitro and in vivo model.
32702056	3	46	theme	basic	494:498	arg1	data					516:519	basic and fundamental data	494:519	basic and fundamental data on impacts of a breadfruit-based diet	494:557	We designed a series of studies to provide basic and fundamental data on impacts of a breadfruit-based diet through an in vitro and in vivo model.
32702056	2	47	from	impacts	381:387	arg1	humans					431:436	humans	431:436	humans	431:436	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	2	47	from	impacts	381:387	arg1	animals					419:425	animals	419:425	animals	419:425	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	11	48	theme	cytokine	1507:1514	arg1	expression					1516:1525	the cytokine expression	1503:1525	the cytokine expression of the mice ileum	1503:1543	The histology and the cytokine expression of the mice ileum from both groups were analyzed and showed similar results.
32702056	3	49	theme	studies	475:481	arg1	series					465:470	a series	463:470	a series of studies	463:481	We designed a series of studies to provide basic and fundamental data on impacts of a breadfruit-based diet through an in vitro and in vivo model.
32702056	8	50	theme	breadfruit-based	1151:1166	arg1	chow					1175:1178	A breadfruit-based rodent chow	1149:1178	A breadfruit-based rodent chow	1149:1178	A breadfruit-based rodent chow was formulated by substitution of all of the wheat in the standard formulation with breadfruit.
32702056	12	51	theme	similar	1674:1680	arg1	results					1682:1688	similar results	1674:1688	similar results	1674:1688	The expression of major bacteria was measured in the colon and showed similar results.
32702056	11	52	theme	mice	1534:1537	arg1	ileum					1539:1543	the mice ileum	1530:1543	the mice ileum	1530:1543	The histology and the cytokine expression of the mice ileum from both groups were analyzed and showed similar results.
32702056	2	53	theme	modern	327:332	arg1	foods					334:338	modern foods	327:338	modern foods	327:338	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	5	54	dep	easier	779:784	arg1	to					786:787	to	786:787	to	786:787	Breadfruit protein was found to be easier to digest than wheat protein in the enzyme digestion model.
32702056	5	54	dep	easier	779:784	arg1	digest					789:794	digest	789:794	digest	789:794	Breadfruit protein was found to be easier to digest than wheat protein in the enzyme digestion model.
32702056	2	55	theme	glycemic	264:271	arg1	Flour					211:215	Flour	211:215	Flour produced from breadfruit	211:240	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	2	55	theme	glycemic	264:271	arg1	option					316:321	nutrient dense and complete protein option	280:321	nutrient dense and complete protein option for modern foods	280:338	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	2	55	theme	glycemic	264:271	arg1	index					273:277	a gluten-free, low glycemic index	245:277	index	273:277	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	9	56	theme	male	1340:1343	arg1	mice					1364:1367	male and female C57BL/6 mice	1340:1367	male and female C57BL/6 mice	1340:1367	The diets were isocaloric, nutrient equivalent and used to feed male and female C57BL/6 mice for 21 days.
32702056	14	57	dep	in	1862:1863	arg1	vitro					1865:1869	vitro	1865:1869	vitro	1865:1869	No negative health outcomes were observed in studies with in vitro or in vivo models and breadfruit flour is a healthy alternative to other starches for modern foods.
32702056	9	58	theme	female	1349:1354	arg1	mice					1364:1367	male and female C57BL/6 mice	1340:1367	male and female C57BL/6 mice	1340:1367	The diets were isocaloric, nutrient equivalent and used to feed male and female C57BL/6 mice for 21 days.
32702056	4	59	theme	Cooked	598:603	arg1	flour					616:620	Cooked breadfruit flour	598:620	Cooked breadfruit flour	598:620	Cooked breadfruit flour was digested through a multi-stage enzyme digestion model to estimate protein digestibility in comparison to wheat flour.
32702056	2	60	theme	dense	289:293	arg1	option					316:321	nutrient dense and complete protein option	280:321	nutrient dense and complete protein option for modern foods	280:338	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	2	60	theme	dense	289:293	arg1	index					273:277	a gluten-free, low glycemic index	245:277	index	273:277	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	14	61	theme	breadfruit	1893:1902	arg1	flour					1904:1908	breadfruit flour	1893:1908	breadfruit flour	1893:1908	No negative health outcomes were observed in studies with in vitro or in vivo models and breadfruit flour is a healthy alternative to other starches for modern foods.
32702056	14	61	theme	breadfruit	1893:1902	arg1	alternative					1923:1933	a healthy alternative	1913:1933	a healthy alternative to other starches for modern foods	1913:1968	No negative health outcomes were observed in studies with in vitro or in vivo models and breadfruit flour is a healthy alternative to other starches for modern foods.
32702056	12	62	theme	bacteria	1628:1635	arg1	expression					1608:1617	The expression	1604:1617	The expression of major bacteria	1604:1635	The expression of major bacteria was measured in the colon and showed similar results.
32702056	11	63	dep	histology	1489:1497	arg1	The					1485:1487	The	1485:1487	The	1485:1487	The histology and the cytokine expression of the mice ileum from both groups were analyzed and showed similar results.
32702056	10	64	theme	diet	1479:1482	arg1	mice					1459:1462	the mice	1455:1462	the mice because of the diet	1455:1482	No sign of malnutrition, discomfort, illness or death was observed among the mice because of the diet.
32702056	7	65	theme	immune	1029:1034	arg1	factors					1036:1042	immune factors	1029:1042	immune factors	1029:1042	No significant differences were observed for immune factors and cytokines (IL-4, IL-10, IL-8, TNF-α, IFN-γ) on Caco-2 cells between the breadfruit and wheat groups.
32702056	1	66	theme	food	174:177	arg1	security					179:186	worldwide food security	164:186	worldwide food security	164:186	Breadfruit is a traditional staple crop from Pacific islands with the potential to improve worldwide food security and mitigate diabetes.
32702056	3	67	theme	diet	554:557	arg1	impacts					524:530	impacts	524:530	impacts of a breadfruit-based diet	524:557	We designed a series of studies to provide basic and fundamental data on impacts of a breadfruit-based diet through an in vitro and in vivo model.
32702056	5	68	theme	enzyme	822:827	arg1	model					839:843	the enzyme digestion model	818:843	the enzyme digestion model	818:843	Breadfruit protein was found to be easier to digest than wheat protein in the enzyme digestion model.
32702056	1	69	theme	traditional	89:99	arg1	crop					108:111	a traditional staple crop	87:111	a traditional staple crop from Pacific islands with the potential to improve worldwide food security and mitigate diabetes	87:208	Breadfruit is a traditional staple crop from Pacific islands with the potential to improve worldwide food security and mitigate diabetes.
32702056	1	69	theme	traditional	89:99	arg1	Breadfruit					73:82	Breadfruit	73:82	Breadfruit	73:82	Breadfruit is a traditional staple crop from Pacific islands with the potential to improve worldwide food security and mitigate diabetes.
32702056	6	70	theme	Caco-2	883:888	arg1	cells					890:894	Caco-2 cells	883:894	Caco-2 cells	883:894	The flour digestions were applied to Caco-2 cells to test the cytotoxicity and to measure the immunogenicity through cytokine expression.
32702056	13	71	contain	had	1720:1722	arg2	rate					1754:1757	a significantly higher growth rate	1724:1757	a significantly higher growth rate	1724:1757	Mice fed the breadfruit diet had a significantly higher growth rate and body weight than standard diet fed mice.
32702056	13	71	contain	had	1720:1722	arg2	weight					1768:1773	body weight	1763:1773	body weight	1763:1773	Mice fed the breadfruit diet had a significantly higher growth rate and body weight than standard diet fed mice.
32702056	13	71	contain	had	1720:1722	arg1	Mice					1691:1694	Mice	1691:1694	Mice fed the breadfruit diet	1691:1718	Mice fed the breadfruit diet had a significantly higher growth rate and body weight than standard diet fed mice.
32702056	8	72	theme	all	1214:1216	arg1	substitution					1198:1209	substitution	1198:1209	substitution of all of the wheat in the standard formulation with breadfruit	1198:1273	A breadfruit-based rodent chow was formulated by substitution of all of the wheat in the standard formulation with breadfruit.
32702056	3	73	from	data	516:519	arg1	impacts					524:530	impacts	524:530	impacts of a breadfruit-based diet	524:557	We designed a series of studies to provide basic and fundamental data on impacts of a breadfruit-based diet through an in vitro and in vivo model.
32702056	14	74	theme	negative	1807:1814	arg1	outcomes					1823:1830	No negative health outcomes	1804:1830	No negative health outcomes	1804:1830	No negative health outcomes were observed in studies with in vitro or in vivo models and breadfruit flour is a healthy alternative to other starches for modern foods.
32702056	13	75	theme	diet	1789:1792	arg1	mice					1798:1801	standard diet fed mice	1780:1801	standard diet fed mice	1780:1801	Mice fed the breadfruit diet had a significantly higher growth rate and body weight than standard diet fed mice.
32702056	8	76	from	substitution	1198:1209	arg1	formulation					1247:1257	the standard formulation	1234:1257	the standard formulation with breadfruit	1234:1273	A breadfruit-based rodent chow was formulated by substitution of all of the wheat in the standard formulation with breadfruit.
32702056	5	77	theme	digestion	829:837	arg1	model					839:843	the enzyme digestion model	818:843	the enzyme digestion model	818:843	Breadfruit protein was found to be easier to digest than wheat protein in the enzyme digestion model.
32702056	6	78	theme	cytokine	963:970	arg1	expression					972:981	cytokine expression	963:981	cytokine expression	963:981	The flour digestions were applied to Caco-2 cells to test the cytotoxicity and to measure the immunogenicity through cytokine expression.
32702056	2	79	from	diet	411:414	arg1	humans					431:436	humans	431:436	humans	431:436	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	2	79	from	diet	411:414	arg1	animals					419:425	animals	419:425	animals	419:425	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	7	80	theme	wheat	1135:1139	arg1	groups					1141:1146	the breadfruit and wheat groups	1116:1146	groups	1141:1146	No significant differences were observed for immune factors and cytokines (IL-4, IL-10, IL-8, TNF-α, IFN-γ) on Caco-2 cells between the breadfruit and wheat groups.
32702056	2	81	from	animals	419:425	arg1	impacts					381:387	health impacts	374:387	health impacts of a breadfruit-based diet in animals and humans	374:436	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	8	82	theme	standard	1238:1245	arg1	formulation					1247:1257	the standard formulation	1234:1257	the standard formulation with breadfruit	1234:1273	A breadfruit-based rodent chow was formulated by substitution of all of the wheat in the standard formulation with breadfruit.
32702056	13	83	theme	body	1763:1766	arg1	weight					1768:1773	body weight	1763:1773	body weight	1763:1773	Mice fed the breadfruit diet had a significantly higher growth rate and body weight than standard diet fed mice.
32702056	7	84	theme	breadfruit	1120:1129	arg1	groups					1141:1146	the breadfruit and wheat groups	1116:1146	groups	1141:1146	No significant differences were observed for immune factors and cytokines (IL-4, IL-10, IL-8, TNF-α, IFN-γ) on Caco-2 cells between the breadfruit and wheat groups.
32702056	2	85	from	humans	431:436	arg1	impacts					381:387	health impacts	374:387	health impacts of a breadfruit-based diet in animals and humans	374:436	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	14	86	theme	modern	1957:1962	arg1	foods					1964:1968	modern foods	1957:1968	modern foods	1957:1968	No negative health outcomes were observed in studies with in vitro or in vivo models and breadfruit flour is a healthy alternative to other starches for modern foods.
32702056	5	87	from	easier	779:784	arg1	model					839:843	the enzyme digestion model	818:843	the enzyme digestion model	818:843	Breadfruit protein was found to be easier to digest than wheat protein in the enzyme digestion model.
32702056	1	88	with	crop	108:111	arg1	potential					143:151	the potential to improve worldwide food security and mitigate diabetes	139:208	the potential to improve worldwide food security and mitigate diabetes	139:208	Breadfruit is a traditional staple crop from Pacific islands with the potential to improve worldwide food security and mitigate diabetes.
32702056	1	89	from	islands	126:132	arg1	crop					108:111	a traditional staple crop	87:111	a traditional staple crop from Pacific islands with the potential to improve worldwide food security and mitigate diabetes	87:208	Breadfruit is a traditional staple crop from Pacific islands with the potential to improve worldwide food security and mitigate diabetes.
32702056	1	89	from	islands	126:132	arg1	Breadfruit					73:82	Breadfruit	73:82	Breadfruit	73:82	Breadfruit is a traditional staple crop from Pacific islands with the potential to improve worldwide food security and mitigate diabetes.
32702056	4	90	theme	wheat	731:735	arg1	flour					737:741	wheat flour	731:741	wheat flour	731:741	Cooked breadfruit flour was digested through a multi-stage enzyme digestion model to estimate protein digestibility in comparison to wheat flour.
32702056	13	91	theme	higher	1740:1745	arg1	rate					1754:1757	a significantly higher growth rate	1724:1757	a significantly higher growth rate	1724:1757	Mice fed the breadfruit diet had a significantly higher growth rate and body weight than standard diet fed mice.
32702056	7	92	theme	Caco-2	1095:1100	arg1	cells					1102:1106	Caco-2 cells	1095:1106	Caco-2 cells	1095:1106	No significant differences were observed for immune factors and cytokines (IL-4, IL-10, IL-8, TNF-α, IFN-γ) on Caco-2 cells between the breadfruit and wheat groups.
32702056	14	93	theme	in	1862:1863	arg1	models					1882:1887	in vitro or in vivo models	1862:1887	in vitro or in vivo models	1862:1887	No negative health outcomes were observed in studies with in vitro or in vivo models and breadfruit flour is a healthy alternative to other starches for modern foods.
32702056	2	94	theme	basic	344:348	arg1	knowledge					361:369	basic scientific knowledge	344:369	basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans	344:436	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	11	95	from	groups	1555:1560	arg1	expression					1516:1525	the cytokine expression	1503:1525	the cytokine expression of the mice ileum	1503:1543	The histology and the cytokine expression of the mice ileum from both groups were analyzed and showed similar results.
32702056	11	95	from	groups	1555:1560	arg1	histology					1489:1497	histology	1489:1497	histology	1489:1497	The histology and the cytokine expression of the mice ileum from both groups were analyzed and showed similar results.
32702056	10	96	theme	malnutrition	1393:1404	arg1	sign					1385:1388	No sign	1382:1388	No sign of malnutrition, discomfort, illness or death	1382:1434	No sign of malnutrition, discomfort, illness or death was observed among the mice because of the diet.
32702056	3	97	theme	in	583:584	arg1	model					591:595	an in vitro and in vivo model	567:595	an in vitro and in vivo model	567:595	We designed a series of studies to provide basic and fundamental data on impacts of a breadfruit-based diet through an in vitro and in vivo model.
32702056	2	98	theme	health	374:379	arg1	impacts					381:387	health impacts	374:387	health impacts of a breadfruit-based diet in animals and humans	374:436	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	0	99	theme	modern	41:46	arg1	foods					48:52	modern foods	41:52	modern foods	41:52	Breadfruit flour is a healthy option for modern foods and food security.
32702056	10	100	theme	illness	1419:1425	arg1	sign					1385:1388	No sign	1382:1388	No sign of malnutrition, discomfort, illness or death	1382:1434	No sign of malnutrition, discomfort, illness or death was observed among the mice because of the diet.
32702056	8	101	with	formulation	1247:1257	arg1	breadfruit					1264:1273	breadfruit	1264:1273	breadfruit	1264:1273	A breadfruit-based rodent chow was formulated by substitution of all of the wheat in the standard formulation with breadfruit.
32702056	2	102	theme	protein	308:314	arg1	option					316:321	nutrient dense and complete protein option	280:321	nutrient dense and complete protein option for modern foods	280:338	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	2	102	theme	protein	308:314	arg1	index					273:277	a gluten-free, low glycemic index	245:277	index	273:277	Flour produced from breadfruit is a gluten-free, low glycemic index, nutrient dense and complete protein option for modern foods but basic scientific knowledge of health impacts of a breadfruit-based diet in animals and humans was lacking.
32702056	5	103	theme	Breadfruit	744:753	arg1	protein					755:761	Breadfruit protein	744:761	Breadfruit protein	744:761	Breadfruit protein was found to be easier to digest than wheat protein in the enzyme digestion model.
32702056	10	104	theme	discomfort	1407:1416	arg1	sign					1385:1388	No sign	1382:1388	No sign of malnutrition, discomfort, illness or death	1382:1434	No sign of malnutrition, discomfort, illness or death was observed among the mice because of the diet.
32702056	11	105	theme	similar	1587:1593	arg1	results					1595:1601	similar results	1587:1601	similar results	1587:1601	The histology and the cytokine expression of the mice ileum from both groups were analyzed and showed similar results.
32564953	4	0	theme	gene-based	816:825	arg1	methods					827:833	gene-based methods	816:833	gene-based methods	816:833	Among the statistical methods used to identify epistatic interactions in genome-wide qualitative association studies (GWAS), gene-based methods have recently grown in popularity due to their better statistical power and biological interpretability.
32564953	6	1	theme	milk	1221:1224	arg1	yield					1226:1230	milk yield	1221:1230	milk yield	1221:1230	Thus, the aims of this study were to quantify the epistatic relationships among 48 SNP in the casein complex on the expression of milk yield and components (fat, protein, dry matter, lactose, and somatic cells) in Murciano-Granadina goats, to explain the qualitative nature of the SNP used to quantify the genotypes produced as a result.
32564953	10	2	theme	SNP	1980:1982	arg1	groups					1943:1948	Two partially overlapping groups	1917:1948	Two partially overlapping groups of epistatically interrelated SNP	1917:1982	Two partially overlapping groups of epistatically interrelated SNP were detected: one group of 21 SNP, explaining 57.56% of variability, and another group of 20 SNP, explaining 42.43% (multiple fit ≥ 0.1).
32564953	10	2	theme	SNP	1980:1982	arg1	SNP					1980:1982	epistatically interrelated SNP	1953:1982	epistatically interrelated SNP	1953:1982	Two partially overlapping groups of epistatically interrelated SNP were detected: one group of 21 SNP, explaining 57.56% of variability, and another group of 20 SNP, explaining 42.43% (multiple fit ≥ 0.1).
32564953	11	3	theme	significant	2215:2225	arg1	36					2152:2153	36	2152:2153	36	2152:2153	|Additionally, SNP18, 32, and 36 (CSN1S2, CSN1S1, and CSN2 loci, respectively) were the most significant SNP to explain intragroup epistatic variability (component loading > ||0.5||).|
32564953	11	3	theme	significant	2215:2225	arg1	32					2144:2145	32	2144:2145	32	2144:2145	|Additionally, SNP18, 32, and 36 (CSN1S2, CSN1S1, and CSN2 loci, respectively) were the most significant SNP to explain intragroup epistatic variability (component loading > ||0.5||).|
32564953	11	3	theme	significant	2215:2225	arg1	SNP					2227:2229	|the most significant SNP to explain intragroup epistatic variability (component loading > ||0.5||)|	2206:2301	|the most significant SNP to explain intragroup epistatic variability (component loading > ||0.5||)|	2206:2301	|Additionally, SNP18, 32, and 36 (CSN1S2, CSN1S1, and CSN2 loci, respectively) were the most significant SNP to explain intragroup epistatic variability (component loading > ||0.5||).|
32564953	11	3	theme	significant	2215:2225	arg1	SNP18					2137:2141	SNP18	2137:2141	SNP18	2137:2141	|Additionally, SNP18, 32, and 36 (CSN1S2, CSN1S1, and CSN2 loci, respectively) were the most significant SNP to explain intragroup epistatic variability (component loading > ||0.5||).|
32564953	1	4	theme	traits	283:288	arg1	expression					235:244	the expression	231:244	the expression of milk quantitative and qualitative traits and their interconnections	231:315	Improving knowledge on the causative polymorphisms or genes regulating the expression of milk quantitative and qualitative traits and their interconnections plays a major role in dairy goat breeding programs and genomic research.
32564953	12	5	theme	such	2492:2495	arg1	genes					2497:2501	such genes	2492:2501	such genes	2492:2501	Conclusively, milk yield and quality may not only depend on the specific casein gene pool of individuals, but may also be relevantly conditioned by the relationships set across and within such genes.
32564953	11	6	dep	36	2152:2153	arg1	CSN1S1					2164:2169	CSN1S1	2164:2169	CSN1S1	2164:2169	|Additionally, SNP18, 32, and 36 (CSN1S2, CSN1S1, and CSN2 loci, respectively) were the most significant SNP to explain intragroup epistatic variability (component loading > ||0.5||).|
32564953	11	6	dep	36	2152:2153	arg1	CSN1S2					2156:2161	CSN1S2	2156:2161	CSN1S2	2156:2161	|Additionally, SNP18, 32, and 36 (CSN1S2, CSN1S1, and CSN2 loci, respectively) were the most significant SNP to explain intragroup epistatic variability (component loading > ||0.5||).|
32564953	11	6	dep	36	2152:2153	arg1	loci					2181:2184	CSN2 loci	2176:2184	CSN2 loci	2176:2184	|Additionally, SNP18, 32, and 36 (CSN1S2, CSN1S1, and CSN2 loci, respectively) were the most significant SNP to explain intragroup epistatic variability (component loading > ||0.5||).|
32564953	6	7	dep	components	1236:1245	arg1	protein					1253:1259	protein	1253:1259	protein	1253:1259	Thus, the aims of this study were to quantify the epistatic relationships among 48 SNP in the casein complex on the expression of milk yield and components (fat, protein, dry matter, lactose, and somatic cells) in Murciano-Granadina goats, to explain the qualitative nature of the SNP used to quantify the genotypes produced as a result.
32564953	6	7	dep	components	1236:1245	arg1	cells					1295:1299	somatic cells	1287:1299	somatic cells	1287:1299	Thus, the aims of this study were to quantify the epistatic relationships among 48 SNP in the casein complex on the expression of milk yield and components (fat, protein, dry matter, lactose, and somatic cells) in Murciano-Granadina goats, to explain the qualitative nature of the SNP used to quantify the genotypes produced as a result.
32564953	6	7	dep	components	1236:1245	arg1	components					1236:1245	components	1236:1245	components (fat, protein, dry matter, lactose, and somatic cells)	1236:1300	Thus, the aims of this study were to quantify the epistatic relationships among 48 SNP in the casein complex on the expression of milk yield and components (fat, protein, dry matter, lactose, and somatic cells) in Murciano-Granadina goats, to explain the qualitative nature of the SNP used to quantify the genotypes produced as a result.
32564953	6	7	dep	components	1236:1245	arg1	matter					1266:1271	dry matter	1262:1271	dry matter	1262:1271	Thus, the aims of this study were to quantify the epistatic relationships among 48 SNP in the casein complex on the expression of milk yield and components (fat, protein, dry matter, lactose, and somatic cells) in Murciano-Granadina goats, to explain the qualitative nature of the SNP used to quantify the genotypes produced as a result.
32564953	6	7	dep	components	1236:1245	arg1	fat					1248:1250	fat	1248:1250	fat	1248:1250	Thus, the aims of this study were to quantify the epistatic relationships among 48 SNP in the casein complex on the expression of milk yield and components (fat, protein, dry matter, lactose, and somatic cells) in Murciano-Granadina goats, to explain the qualitative nature of the SNP used to quantify the genotypes produced as a result.
32564953	6	7	dep	components	1236:1245	arg1	lactose					1274:1280	lactose	1274:1280	lactose	1274:1280	Thus, the aims of this study were to quantify the epistatic relationships among 48 SNP in the casein complex on the expression of milk yield and components (fat, protein, dry matter, lactose, and somatic cells) in Murciano-Granadina goats, to explain the qualitative nature of the SNP used to quantify the genotypes produced as a result.
32564953	6	8	theme	casein	1185:1190	arg1	complex					1192:1198	the casein complex	1181:1198	the casein complex	1181:1198	Thus, the aims of this study were to quantify the epistatic relationships among 48 SNP in the casein complex on the expression of milk yield and components (fat, protein, dry matter, lactose, and somatic cells) in Murciano-Granadina goats, to explain the qualitative nature of the SNP used to quantify the genotypes produced as a result.
32564953	1	9	theme	causative	187:195	arg1	polymorphisms					197:209	the causative polymorphisms	183:209	the causative polymorphisms	183:209	Improving knowledge on the causative polymorphisms or genes regulating the expression of milk quantitative and qualitative traits and their interconnections plays a major role in dairy goat breeding programs and genomic research.
32564953	0	10	theme	yield	109:113	arg1	expression					90:99	phenotypic expression	79:99	phenotypic expression of milk yield and composition in Murciano-Granadina goats	79:157	Nonparametric analysis of casein complex genes' epistasis and their effects on phenotypic expression of milk yield and composition in Murciano-Granadina goats.
32564953	5	11	theme	relationships	1022:1034	arg1	magnitude					1005:1013	the magnitude	1001:1013	the magnitude of the relationships between SNP and phenotype	1001:1060	However, most of these methods make strong assumptions about the magnitude of the relationships between SNP and phenotype, limiting statistical power.
32564953	0	12	from	effects	68:74	arg1	expression					90:99	phenotypic expression	79:99	phenotypic expression of milk yield and composition in Murciano-Granadina goats	79:157	Nonparametric analysis of casein complex genes' epistasis and their effects on phenotypic expression of milk yield and composition in Murciano-Granadina goats.
32564953	10	13	theme	SNP	2015:2017	arg1	group					2066:2070	another group	2058:2070	another group of 20 SNP	2058:2080	Two partially overlapping groups of epistatically interrelated SNP were detected: one group of 21 SNP, explaining 57.56% of variability, and another group of 20 SNP, explaining 42.43% (multiple fit ≥ 0.1).
32564953	10	13	theme	SNP	2015:2017	arg1	group					2003:2007	one group	1999:2007	one group of 21 SNP, explaining 57.56% of variability,	1999:2052	Two partially overlapping groups of epistatically interrelated SNP were detected: one group of 21 SNP, explaining 57.56% of variability, and another group of 20 SNP, explaining 42.43% (multiple fit ≥ 0.1).
32564953	10	13	theme	SNP	2015:2017	arg1	SNP					2015:2017	21 SNP	2012:2017	21 SNP	2012:2017	Two partially overlapping groups of epistatically interrelated SNP were detected: one group of 21 SNP, explaining 57.56% of variability, and another group of 20 SNP, explaining 42.43% (multiple fit ≥ 0.1).
32564953	10	13	theme	SNP	2015:2017	arg1	SNP					2078:2080	20 SNP	2075:2080	20 SNP	2075:2080	Two partially overlapping groups of epistatically interrelated SNP were detected: one group of 21 SNP, explaining 57.56% of variability, and another group of 20 SNP, explaining 42.43% (multiple fit ≥ 0.1).
32564953	0	14	theme	composition	119:129	arg1	expression					90:99	phenotypic expression	79:99	phenotypic expression of milk yield and composition in Murciano-Granadina goats	79:157	Nonparametric analysis of casein complex genes' epistasis and their effects on phenotypic expression of milk yield and composition in Murciano-Granadina goats.
32564953	7	15	dep	used	1483:1486	arg1	studied					1527:1533	studied	1527:1533	studied depending on their implications in the explanation of milk yield and components variability	1527:1625	Categorical principal component analysis (CATPCA) was used to delimit and group the number of SNP studied depending on their implications in the explanation of milk yield and components variability.
32564953	8	16	theme	canonical	1649:1657	arg1	analysis					1671:1678	nonlinear canonical correlation analysis	1639:1678	nonlinear canonical correlation analysis	1639:1678	Afterward, nonlinear canonical correlation analysis was used to identify relationships among and within the SNP groups detected by CATPCA.
32564953	10	17	theme	variability	2041:2051	arg1	variability					2041:2051	variability	2041:2051	variability	2041:2051	Two partially overlapping groups of epistatically interrelated SNP were detected: one group of 21 SNP, explaining 57.56% of variability, and another group of 20 SNP, explaining 42.43% (multiple fit ≥ 0.1).
32564953	10	17	theme	variability	2041:2051	arg1	%					2036:2036	57.56%	2031:2036	57.56% of variability	2031:2051	Two partially overlapping groups of epistatically interrelated SNP were detected: one group of 21 SNP, explaining 57.56% of variability, and another group of 20 SNP, explaining 42.43% (multiple fit ≥ 0.1).
32564953	0	18	theme	Murciano-Granadina	134:151	arg1	goats					153:157	Murciano-Granadina goats	134:157	Murciano-Granadina goats	134:157	Nonparametric analysis of casein complex genes' epistasis and their effects on phenotypic expression of milk yield and composition in Murciano-Granadina goats.
32564953	3	19	dep	proteins	565:572	arg1	κ					592:592	κ	592:592	κ	592:592	Goat milk casein proteins (αS1, αS2, β, and κ) are encoded by 4 loci (CSN1S1, CSN1S2, CSN2, and CSN3) clustered within 250 kb on chromosome 6.
32564953	3	19	dep	proteins	565:572	arg1	αS2					580:582	αS2	580:582	αS2	580:582	Goat milk casein proteins (αS1, αS2, β, and κ) are encoded by 4 loci (CSN1S1, CSN1S2, CSN2, and CSN3) clustered within 250 kb on chromosome 6.
32564953	3	19	dep	proteins	565:572	arg1	αS1					575:577	αS1	575:577	αS1	575:577	Goat milk casein proteins (αS1, αS2, β, and κ) are encoded by 4 loci (CSN1S1, CSN1S2, CSN2, and CSN3) clustered within 250 kb on chromosome 6.
32564953	3	19	dep	proteins	565:572	arg1	proteins					565:572	Goat milk casein proteins	548:572	Goat milk casein proteins (αS1, αS2, β, and κ)	548:593	Goat milk casein proteins (αS1, αS2, β, and κ) are encoded by 4 loci (CSN1S1, CSN1S2, CSN2, and CSN3) clustered within 250 kb on chromosome 6.
32564953	3	19	dep	proteins	565:572	arg1	β					585:585	β	585:585	β	585:585	Goat milk casein proteins (αS1, αS2, β, and κ) are encoded by 4 loci (CSN1S1, CSN1S2, CSN2, and CSN3) clustered within 250 kb on chromosome 6.
32564953	4	20	from	interactions	748:759	arg1	GWAS					809:812	GWAS	809:812	GWAS	809:812	Among the statistical methods used to identify epistatic interactions in genome-wide qualitative association studies (GWAS), gene-based methods have recently grown in popularity due to their better statistical power and biological interpretability.
32564953	4	20	from	interactions	748:759	arg1	studies					800:806	genome-wide qualitative association studies	764:806	genome-wide qualitative association studies (GWAS)	764:813	Among the statistical methods used to identify epistatic interactions in genome-wide qualitative association studies (GWAS), gene-based methods have recently grown in popularity due to their better statistical power and biological interpretability.
32564953	9	21	from	%	1797:1797	arg1	traits					1821:1826	the traits	1817:1826	the traits evaluated	1817:1836	Our results suggest that 79.65% of variability in the traits evaluated may be ascribed to the epistatic relationships across and within 7 SNP groups.
32564953	11	22	theme	intragroup	2242:2251	arg1	|||0.5|||					2296:2300	|component loading > ||0.5|||	2276:2300	|component loading > ||0.5|||	2276:2300	|Additionally, SNP18, 32, and 36 (CSN1S2, CSN1S1, and CSN2 loci, respectively) were the most significant SNP to explain intragroup epistatic variability (component loading > ||0.5||).|
32564953	11	22	theme	intragroup	2242:2251	arg1	variability					2263:2273	intragroup epistatic variability	2242:2273	|intragroup epistatic variability (component loading > ||0.5||)|	2242:2301	|Additionally, SNP18, 32, and 36 (CSN1S2, CSN1S1, and CSN2 loci, respectively) were the most significant SNP to explain intragroup epistatic variability (component loading > ||0.5||).|
32564953	1	23	theme	dairy	339:343	arg1	programs					359:366	dairy goat breeding programs	339:366	dairy goat breeding programs	339:366	Improving knowledge on the causative polymorphisms or genes regulating the expression of milk quantitative and qualitative traits and their interconnections plays a major role in dairy goat breeding programs and genomic research.
32564953	1	24	from	knowledge	170:178	arg1	genes					214:218	genes	214:218	genes regulating the expression of milk quantitative and qualitative traits and their interconnections	214:315	Improving knowledge on the causative polymorphisms or genes regulating the expression of milk quantitative and qualitative traits and their interconnections plays a major role in dairy goat breeding programs and genomic research.
32564953	1	24	from	knowledge	170:178	arg1	polymorphisms					197:209	the causative polymorphisms	183:209	the causative polymorphisms	183:209	Improving knowledge on the causative polymorphisms or genes regulating the expression of milk quantitative and qualitative traits and their interconnections plays a major role in dairy goat breeding programs and genomic research.
32564953	2	25	theme	market	515:520	arg1	demands					522:528	market demands	515:528	market demands	515:528	This information enables optimization of predictive and selective tools, to obtain better-performing animals to help satisfy market demands more efficiently.
32564953	11	26	theme	component	2276:2284	arg1	|||0.5|||					2296:2300	|component loading > ||0.5|||	2276:2300	|component loading > ||0.5|||	2276:2300	|Additionally, SNP18, 32, and 36 (CSN1S2, CSN1S1, and CSN2 loci, respectively) were the most significant SNP to explain intragroup epistatic variability (component loading > ||0.5||).|
32564953	11	26	theme	component	2276:2284	arg1	variability					2263:2273	intragroup epistatic variability	2242:2273	|intragroup epistatic variability (component loading > ||0.5||)|	2242:2301	|Additionally, SNP18, 32, and 36 (CSN1S2, CSN1S1, and CSN2 loci, respectively) were the most significant SNP to explain intragroup epistatic variability (component loading > ||0.5||).|
32564953	12	27	theme	individuals	2397:2407	arg1	pool					2389:2392	the specific casein gene pool	2364:2392	the specific casein gene pool of individuals	2364:2407	Conclusively, milk yield and quality may not only depend on the specific casein gene pool of individuals, but may also be relevantly conditioned by the relationships set across and within such genes.
32564953	10	28	theme	overlapping	1931:1941	arg1	groups					1943:1948	Two partially overlapping groups	1917:1948	Two partially overlapping groups of epistatically interrelated SNP	1917:1982	Two partially overlapping groups of epistatically interrelated SNP were detected: one group of 21 SNP, explaining 57.56% of variability, and another group of 20 SNP, explaining 42.43% (multiple fit ≥ 0.1).
32564953	10	28	theme	overlapping	1931:1941	arg1	SNP					1980:1982	epistatically interrelated SNP	1953:1982	epistatically interrelated SNP	1953:1982	Two partially overlapping groups of epistatically interrelated SNP were detected: one group of 21 SNP, explaining 57.56% of variability, and another group of 20 SNP, explaining 42.43% (multiple fit ≥ 0.1).
32564953	1	29	theme	breeding	350:357	arg1	programs					359:366	dairy goat breeding programs	339:366	dairy goat breeding programs	339:366	Improving knowledge on the causative polymorphisms or genes regulating the expression of milk quantitative and qualitative traits and their interconnections plays a major role in dairy goat breeding programs and genomic research.
32564953	1	30	theme	Improving	160:168	arg1	knowledge					170:178	Improving knowledge	160:178	Improving knowledge on the causative polymorphisms or genes regulating the expression of milk quantitative and qualitative traits and their interconnections	160:315	Improving knowledge on the causative polymorphisms or genes regulating the expression of milk quantitative and qualitative traits and their interconnections plays a major role in dairy goat breeding programs and genomic research.
32564953	7	31	theme	milk	1589:1592	arg1	yield					1594:1598	milk yield	1589:1598	milk yield	1589:1598	Categorical principal component analysis (CATPCA) was used to delimit and group the number of SNP studied depending on their implications in the explanation of milk yield and components variability.
32564953	7	32	used	used	1483:1486	arg2	CATPCA					1471:1476	CATPCA	1471:1476	CATPCA	1471:1476	Categorical principal component analysis (CATPCA) was used to delimit and group the number of SNP studied depending on their implications in the explanation of milk yield and components variability.
32564953	7	32	used	used	1483:1486	arg2	analysis					1461:1468	Categorical principal component analysis	1429:1468	Categorical principal component analysis (CATPCA)	1429:1477	Categorical principal component analysis (CATPCA) was used to delimit and group the number of SNP studied depending on their implications in the explanation of milk yield and components variability.
32564953	11	33	theme	CSN2	2176:2179	arg1	loci					2181:2184	CSN2 loci	2176:2184	CSN2 loci	2176:2184	|Additionally, SNP18, 32, and 36 (CSN1S2, CSN1S1, and CSN2 loci, respectively) were the most significant SNP to explain intragroup epistatic variability (component loading > ||0.5||).|
32564953	4	34	theme	statistical	701:711	arg1	methods					713:719	the statistical methods	697:719	the statistical methods used to identify epistatic interactions in genome-wide qualitative association studies (GWAS)	697:813	Among the statistical methods used to identify epistatic interactions in genome-wide qualitative association studies (GWAS), gene-based methods have recently grown in popularity due to their better statistical power and biological interpretability.
32564953	8	35	used	used	1684:1687	arg2	analysis					1671:1678	nonlinear canonical correlation analysis	1639:1678	nonlinear canonical correlation analysis	1639:1678	Afterward, nonlinear canonical correlation analysis was used to identify relationships among and within the SNP groups detected by CATPCA.
32564953	0	36	theme	phenotypic	79:88	arg1	expression					90:99	phenotypic expression	79:99	phenotypic expression of milk yield and composition in Murciano-Granadina goats	79:157	Nonparametric analysis of casein complex genes' epistasis and their effects on phenotypic expression of milk yield and composition in Murciano-Granadina goats.
32564953	4	37	theme	biological	911:920	arg1	interpretability					922:937	biological interpretability	911:937	biological interpretability	911:937	Among the statistical methods used to identify epistatic interactions in genome-wide qualitative association studies (GWAS), gene-based methods have recently grown in popularity due to their better statistical power and biological interpretability.
32564953	12	38	theme	casein	2377:2382	arg1	pool					2389:2392	the specific casein gene pool	2364:2392	the specific casein gene pool of individuals	2364:2407	Conclusively, milk yield and quality may not only depend on the specific casein gene pool of individuals, but may also be relevantly conditioned by the relationships set across and within such genes.
32564953	8	39	theme	SNP	1736:1738	arg1	groups					1740:1745	the SNP groups	1732:1745	the SNP groups detected by CATPCA	1732:1764	Afterward, nonlinear canonical correlation analysis was used to identify relationships among and within the SNP groups detected by CATPCA.
32564953	11	40	theme	>	2294:2294	arg1	|||0.5|||					2296:2300	|component loading > ||0.5|||	2276:2300	|component loading > ||0.5|||	2276:2300	|Additionally, SNP18, 32, and 36 (CSN1S2, CSN1S1, and CSN2 loci, respectively) were the most significant SNP to explain intragroup epistatic variability (component loading > ||0.5||).|
32564953	11	40	theme	>	2294:2294	arg1	variability					2263:2273	intragroup epistatic variability	2242:2273	|intragroup epistatic variability (component loading > ||0.5||)|	2242:2301	|Additionally, SNP18, 32, and 36 (CSN1S2, CSN1S1, and CSN2 loci, respectively) were the most significant SNP to explain intragroup epistatic variability (component loading > ||0.5||).|
32564953	7	41	dep	yield	1594:1598	arg1	variability					1615:1625	variability	1615:1625	variability	1615:1625	Categorical principal component analysis (CATPCA) was used to delimit and group the number of SNP studied depending on their implications in the explanation of milk yield and components variability.
32564953	0	42	theme	Nonparametric	0:12	arg1	analysis					14:21	Nonparametric analysis	0:21	Nonparametric analysis of casein complex genes' epistasis and their effects on phenotypic expression of milk yield and composition in Murciano-Granadina goats.	0:158	Nonparametric analysis of casein complex genes' epistasis and their effects on phenotypic expression of milk yield and composition in Murciano-Granadina goats.
32564953	6	43	theme	epistatic	1141:1149	arg1	relationships					1151:1163	the epistatic relationships	1137:1163	the epistatic relationships among 48 SNP	1137:1176	Thus, the aims of this study were to quantify the epistatic relationships among 48 SNP in the casein complex on the expression of milk yield and components (fat, protein, dry matter, lactose, and somatic cells) in Murciano-Granadina goats, to explain the qualitative nature of the SNP used to quantify the genotypes produced as a result.
32564953	2	44	theme	selective	446:454	arg1	tools					456:460	predictive and selective tools	431:460	predictive and selective tools	431:460	This information enables optimization of predictive and selective tools, to obtain better-performing animals to help satisfy market demands more efficiently.
32564953	1	45	theme	interconnections	300:315	arg1	expression					235:244	the expression	231:244	the expression of milk quantitative and qualitative traits and their interconnections	231:315	Improving knowledge on the causative polymorphisms or genes regulating the expression of milk quantitative and qualitative traits and their interconnections plays a major role in dairy goat breeding programs and genomic research.
32564953	7	46	theme	Categorical	1429:1439	arg1	CATPCA					1471:1476	CATPCA	1471:1476	CATPCA	1471:1476	Categorical principal component analysis (CATPCA) was used to delimit and group the number of SNP studied depending on their implications in the explanation of milk yield and components variability.
32564953	7	46	theme	Categorical	1429:1439	arg1	analysis					1461:1468	Categorical principal component analysis	1429:1468	Categorical principal component analysis (CATPCA)	1429:1477	Categorical principal component analysis (CATPCA) was used to delimit and group the number of SNP studied depending on their implications in the explanation of milk yield and components variability.
32564953	4	47	theme	genome-wide	764:774	arg1	GWAS					809:812	GWAS	809:812	GWAS	809:812	Among the statistical methods used to identify epistatic interactions in genome-wide qualitative association studies (GWAS), gene-based methods have recently grown in popularity due to their better statistical power and biological interpretability.
32564953	4	47	theme	genome-wide	764:774	arg1	studies					800:806	genome-wide qualitative association studies	764:806	genome-wide qualitative association studies (GWAS)	764:813	Among the statistical methods used to identify epistatic interactions in genome-wide qualitative association studies (GWAS), gene-based methods have recently grown in popularity due to their better statistical power and biological interpretability.
32564953	2	48	theme	predictive	431:440	arg1	tools					456:460	predictive and selective tools	431:460	predictive and selective tools	431:460	This information enables optimization of predictive and selective tools, to obtain better-performing animals to help satisfy market demands more efficiently.
32564953	0	49	theme	complex	33:39	arg1	genes					41:45	casein complex genes	26:45	casein complex genes' epistasis and their effects on phenotypic expression of milk yield and composition in Murciano-Granadina goats	26:157	Nonparametric analysis of casein complex genes' epistasis and their effects on phenotypic expression of milk yield and composition in Murciano-Granadina goats.
32564953	5	50	theme	strong	976:981	arg1	assumptions					983:993	strong assumptions	976:993	strong assumptions about the magnitude of the relationships between SNP and phenotype	976:1060	However, most of these methods make strong assumptions about the magnitude of the relationships between SNP and phenotype, limiting statistical power.
32564953	4	51	theme	association	788:798	arg1	GWAS					809:812	GWAS	809:812	GWAS	809:812	Among the statistical methods used to identify epistatic interactions in genome-wide qualitative association studies (GWAS), gene-based methods have recently grown in popularity due to their better statistical power and biological interpretability.
32564953	4	51	theme	association	788:798	arg1	studies					800:806	genome-wide qualitative association studies	764:806	genome-wide qualitative association studies (GWAS)	764:813	Among the statistical methods used to identify epistatic interactions in genome-wide qualitative association studies (GWAS), gene-based methods have recently grown in popularity due to their better statistical power and biological interpretability.
32564953	10	52	theme	interrelated	1967:1978	arg1	SNP					1980:1982	epistatically interrelated SNP	1953:1982	epistatically interrelated SNP	1953:1982	Two partially overlapping groups of epistatically interrelated SNP were detected: one group of 21 SNP, explaining 57.56% of variability, and another group of 20 SNP, explaining 42.43% (multiple fit ≥ 0.1).
32564953	3	53	dep	loci	612:615	arg1	CSN1S1					618:623	CSN1S1	618:623	CSN1S1	618:623	Goat milk casein proteins (αS1, αS2, β, and κ) are encoded by 4 loci (CSN1S1, CSN1S2, CSN2, and CSN3) clustered within 250 kb on chromosome 6.
32564953	3	53	dep	loci	612:615	arg1	loci					612:615	4 loci	610:615	4 loci (CSN1S1, CSN1S2, CSN2, and CSN3) clustered within 250 kb on chromosome 6	610:688	Goat milk casein proteins (αS1, αS2, β, and κ) are encoded by 4 loci (CSN1S1, CSN1S2, CSN2, and CSN3) clustered within 250 kb on chromosome 6.
32564953	3	53	dep	loci	612:615	arg1	CSN2					634:637	CSN2	634:637	CSN2	634:637	Goat milk casein proteins (αS1, αS2, β, and κ) are encoded by 4 loci (CSN1S1, CSN1S2, CSN2, and CSN3) clustered within 250 kb on chromosome 6.
32564953	3	53	dep	loci	612:615	arg1	CSN3					644:647	CSN3	644:647	CSN3	644:647	Goat milk casein proteins (αS1, αS2, β, and κ) are encoded by 4 loci (CSN1S1, CSN1S2, CSN2, and CSN3) clustered within 250 kb on chromosome 6.
32564953	3	53	dep	loci	612:615	arg1	CSN1S2					626:631	CSN1S2	626:631	CSN1S2	626:631	Goat milk casein proteins (αS1, αS2, β, and κ) are encoded by 4 loci (CSN1S1, CSN1S2, CSN2, and CSN3) clustered within 250 kb on chromosome 6.
32564953	3	54	theme	Goat	548:551	arg1	αS2					580:582	αS2	580:582	αS2	580:582	Goat milk casein proteins (αS1, αS2, β, and κ) are encoded by 4 loci (CSN1S1, CSN1S2, CSN2, and CSN3) clustered within 250 kb on chromosome 6.
32564953	3	54	theme	Goat	548:551	arg1	αS1					575:577	αS1	575:577	αS1	575:577	Goat milk casein proteins (αS1, αS2, β, and κ) are encoded by 4 loci (CSN1S1, CSN1S2, CSN2, and CSN3) clustered within 250 kb on chromosome 6.
32564953	3	54	theme	Goat	548:551	arg1	proteins					565:572	Goat milk casein proteins	548:572	Goat milk casein proteins (αS1, αS2, β, and κ)	548:593	Goat milk casein proteins (αS1, αS2, β, and κ) are encoded by 4 loci (CSN1S1, CSN1S2, CSN2, and CSN3) clustered within 250 kb on chromosome 6.
32564953	6	55	theme	yield	1226:1230	arg1	expression					1207:1216	the expression	1203:1216	the expression of milk yield and components (fat, protein, dry matter, lactose, and somatic cells) in Murciano-Granadina goats	1203:1328	Thus, the aims of this study were to quantify the epistatic relationships among 48 SNP in the casein complex on the expression of milk yield and components (fat, protein, dry matter, lactose, and somatic cells) in Murciano-Granadina goats, to explain the qualitative nature of the SNP used to quantify the genotypes produced as a result.
32564953	1	56	theme	quantitative	254:265	arg1	traits					283:288	milk quantitative and qualitative traits	249:288	milk quantitative and qualitative traits	249:288	Improving knowledge on the causative polymorphisms or genes regulating the expression of milk quantitative and qualitative traits and their interconnections plays a major role in dairy goat breeding programs and genomic research.
32564953	12	57	theme	milk	2318:2321	arg1	yield					2323:2327	milk yield	2318:2327	milk yield	2318:2327	Conclusively, milk yield and quality may not only depend on the specific casein gene pool of individuals, but may also be relevantly conditioned by the relationships set across and within such genes.
32564953	1	58	theme	qualitative	271:281	arg1	traits					283:288	milk quantitative and qualitative traits	249:288	milk quantitative and qualitative traits	249:288	Improving knowledge on the causative polymorphisms or genes regulating the expression of milk quantitative and qualitative traits and their interconnections plays a major role in dairy goat breeding programs and genomic research.
32564953	10	59	theme	fit	2111:2113	arg1	%					2099:2099	42.43%	2094:2099	42.43% (multiple fit ≥ 0.1)	2094:2120	Two partially overlapping groups of epistatically interrelated SNP were detected: one group of 21 SNP, explaining 57.56% of variability, and another group of 20 SNP, explaining 42.43% (multiple fit ≥ 0.1).
32564953	10	59	theme	fit	2111:2113	arg1	≥					2115:2115	multiple fit ≥ 0.1	2102:2119	multiple fit ≥ 0.1	2102:2119	Two partially overlapping groups of epistatically interrelated SNP were detected: one group of 21 SNP, explaining 57.56% of variability, and another group of 20 SNP, explaining 42.43% (multiple fit ≥ 0.1).
32564953	3	60	theme	casein	558:563	arg1	αS2					580:582	αS2	580:582	αS2	580:582	Goat milk casein proteins (αS1, αS2, β, and κ) are encoded by 4 loci (CSN1S1, CSN1S2, CSN2, and CSN3) clustered within 250 kb on chromosome 6.
32564953	3	60	theme	casein	558:563	arg1	αS1					575:577	αS1	575:577	αS1	575:577	Goat milk casein proteins (αS1, αS2, β, and κ) are encoded by 4 loci (CSN1S1, CSN1S2, CSN2, and CSN3) clustered within 250 kb on chromosome 6.
32564953	3	60	theme	casein	558:563	arg1	proteins					565:572	Goat milk casein proteins	548:572	Goat milk casein proteins (αS1, αS2, β, and κ)	548:593	Goat milk casein proteins (αS1, αS2, β, and κ) are encoded by 4 loci (CSN1S1, CSN1S2, CSN2, and CSN3) clustered within 250 kb on chromosome 6.
32564953	6	61	theme	study	1114:1118	arg1	aims					1101:1104	the aims	1097:1104	the aims of this study	1097:1118	Thus, the aims of this study were to quantify the epistatic relationships among 48 SNP in the casein complex on the expression of milk yield and components (fat, protein, dry matter, lactose, and somatic cells) in Murciano-Granadina goats, to explain the qualitative nature of the SNP used to quantify the genotypes produced as a result.
32564953	9	62	theme	variability	1802:1812	arg1	variability					1802:1812	variability	1802:1812	variability	1802:1812	Our results suggest that 79.65% of variability in the traits evaluated may be ascribed to the epistatic relationships across and within 7 SNP groups.
32564953	9	62	theme	variability	1802:1812	arg1	%					1797:1797	79.65%	1792:1797	79.65% of variability in the traits evaluated	1792:1836	Our results suggest that 79.65% of variability in the traits evaluated may be ascribed to the epistatic relationships across and within 7 SNP groups.
32564953	7	63	theme	SNP	1523:1525	arg1	number					1513:1518	the number	1509:1518	the number of SNP	1509:1525	Categorical principal component analysis (CATPCA) was used to delimit and group the number of SNP studied depending on their implications in the explanation of milk yield and components variability.
32564953	6	64	theme	dry	1262:1264	arg1	matter					1266:1271	dry matter	1262:1271	dry matter	1262:1271	Thus, the aims of this study were to quantify the epistatic relationships among 48 SNP in the casein complex on the expression of milk yield and components (fat, protein, dry matter, lactose, and somatic cells) in Murciano-Granadina goats, to explain the qualitative nature of the SNP used to quantify the genotypes produced as a result.
32564953	6	64	theme	dry	1262:1264	arg1	components					1236:1245	components	1236:1245	components (fat, protein, dry matter, lactose, and somatic cells)	1236:1300	Thus, the aims of this study were to quantify the epistatic relationships among 48 SNP in the casein complex on the expression of milk yield and components (fat, protein, dry matter, lactose, and somatic cells) in Murciano-Granadina goats, to explain the qualitative nature of the SNP used to quantify the genotypes produced as a result.
32564953	8	65	theme	nonlinear	1639:1647	arg1	analysis					1671:1678	nonlinear canonical correlation analysis	1639:1678	nonlinear canonical correlation analysis	1639:1678	Afterward, nonlinear canonical correlation analysis was used to identify relationships among and within the SNP groups detected by CATPCA.
32564953	7	66	from	implications	1554:1565	arg1	explanation					1574:1584	the explanation	1570:1584	the explanation of milk yield and components variability	1570:1625	Categorical principal component analysis (CATPCA) was used to delimit and group the number of SNP studied depending on their implications in the explanation of milk yield and components variability.
32564953	6	67	theme	components	1236:1245	arg1	expression					1207:1216	the expression	1203:1216	the expression of milk yield and components (fat, protein, dry matter, lactose, and somatic cells) in Murciano-Granadina goats	1203:1328	Thus, the aims of this study were to quantify the epistatic relationships among 48 SNP in the casein complex on the expression of milk yield and components (fat, protein, dry matter, lactose, and somatic cells) in Murciano-Granadina goats, to explain the qualitative nature of the SNP used to quantify the genotypes produced as a result.
32564953	8	68	theme	correlation	1659:1669	arg1	analysis					1671:1678	nonlinear canonical correlation analysis	1639:1678	nonlinear canonical correlation analysis	1639:1678	Afterward, nonlinear canonical correlation analysis was used to identify relationships among and within the SNP groups detected by CATPCA.
32564953	0	69	from	expression	90:99	arg1	goats					153:157	Murciano-Granadina goats	134:157	Murciano-Granadina goats	134:157	Nonparametric analysis of casein complex genes' epistasis and their effects on phenotypic expression of milk yield and composition in Murciano-Granadina goats.
32564953	9	70	theme	epistatic	1861:1869	arg1	relationships					1871:1883	the epistatic relationships	1857:1883	the epistatic relationships across and within 7 SNP groups	1857:1914	Our results suggest that 79.65% of variability in the traits evaluated may be ascribed to the epistatic relationships across and within 7 SNP groups.
32564953	6	71	theme	somatic	1287:1293	arg1	cells					1295:1299	somatic cells	1287:1299	somatic cells	1287:1299	Thus, the aims of this study were to quantify the epistatic relationships among 48 SNP in the casein complex on the expression of milk yield and components (fat, protein, dry matter, lactose, and somatic cells) in Murciano-Granadina goats, to explain the qualitative nature of the SNP used to quantify the genotypes produced as a result.
32564953	1	72	theme	goat	345:348	arg1	programs					359:366	dairy goat breeding programs	339:366	dairy goat breeding programs	339:366	Improving knowledge on the causative polymorphisms or genes regulating the expression of milk quantitative and qualitative traits and their interconnections plays a major role in dairy goat breeding programs and genomic research.
32564953	13	73	theme	important	2607:2615	arg1	traits					2623:2628	economically important dairy traits	2594:2628	economically important dairy traits	2594:2628	Hence, studying epistasis in isolation may be crucial to optimize selective practices for economically important dairy traits.
32564953	7	74	theme	components	1604:1613	arg1	explanation					1574:1584	the explanation	1570:1584	the explanation of milk yield and components variability	1570:1625	Categorical principal component analysis (CATPCA) was used to delimit and group the number of SNP studied depending on their implications in the explanation of milk yield and components variability.
32564953	11	75	theme	epistatic	2253:2261	arg1	|||0.5|||					2296:2300	|component loading > ||0.5|||	2276:2300	|component loading > ||0.5|||	2276:2300	|Additionally, SNP18, 32, and 36 (CSN1S2, CSN1S1, and CSN2 loci, respectively) were the most significant SNP to explain intragroup epistatic variability (component loading > ||0.5||).|
32564953	11	75	theme	epistatic	2253:2261	arg1	variability					2263:2273	intragroup epistatic variability	2242:2273	|intragroup epistatic variability (component loading > ||0.5||)|	2242:2301	|Additionally, SNP18, 32, and 36 (CSN1S2, CSN1S1, and CSN2 loci, respectively) were the most significant SNP to explain intragroup epistatic variability (component loading > ||0.5||).|
32564953	12	76	theme	specific	2368:2375	arg1	pool					2389:2392	the specific casein gene pool	2364:2392	the specific casein gene pool of individuals	2364:2407	Conclusively, milk yield and quality may not only depend on the specific casein gene pool of individuals, but may also be relevantly conditioned by the relationships set across and within such genes.
32564953	4	77	dep	better	882:887	arg1	power					901:905	statistical power	889:905	statistical power	889:905	Among the statistical methods used to identify epistatic interactions in genome-wide qualitative association studies (GWAS), gene-based methods have recently grown in popularity due to their better statistical power and biological interpretability.
32564953	10	78	theme	SNP	2078:2080	arg1	group					2066:2070	another group	2058:2070	another group of 20 SNP	2058:2080	Two partially overlapping groups of epistatically interrelated SNP were detected: one group of 21 SNP, explaining 57.56% of variability, and another group of 20 SNP, explaining 42.43% (multiple fit ≥ 0.1).
32564953	10	78	theme	SNP	2078:2080	arg1	group					2003:2007	one group	1999:2007	one group of 21 SNP, explaining 57.56% of variability,	1999:2052	Two partially overlapping groups of epistatically interrelated SNP were detected: one group of 21 SNP, explaining 57.56% of variability, and another group of 20 SNP, explaining 42.43% (multiple fit ≥ 0.1).
32564953	10	78	theme	SNP	2078:2080	arg1	SNP					2015:2017	21 SNP	2012:2017	21 SNP	2012:2017	Two partially overlapping groups of epistatically interrelated SNP were detected: one group of 21 SNP, explaining 57.56% of variability, and another group of 20 SNP, explaining 42.43% (multiple fit ≥ 0.1).
32564953	10	78	theme	SNP	2078:2080	arg1	SNP					2078:2080	20 SNP	2075:2080	20 SNP	2075:2080	Two partially overlapping groups of epistatically interrelated SNP were detected: one group of 21 SNP, explaining 57.56% of variability, and another group of 20 SNP, explaining 42.43% (multiple fit ≥ 0.1).
32564953	7	79	theme	yield	1594:1598	arg1	explanation					1574:1584	the explanation	1570:1584	the explanation of milk yield and components variability	1570:1625	Categorical principal component analysis (CATPCA) was used to delimit and group the number of SNP studied depending on their implications in the explanation of milk yield and components variability.
32564953	7	80	theme	component	1451:1459	arg1	CATPCA					1471:1476	CATPCA	1471:1476	CATPCA	1471:1476	Categorical principal component analysis (CATPCA) was used to delimit and group the number of SNP studied depending on their implications in the explanation of milk yield and components variability.
32564953	7	80	theme	component	1451:1459	arg1	analysis					1461:1468	Categorical principal component analysis	1429:1468	Categorical principal component analysis (CATPCA)	1429:1477	Categorical principal component analysis (CATPCA) was used to delimit and group the number of SNP studied depending on their implications in the explanation of milk yield and components variability.
32564953	4	81	theme	statistical	889:899	arg1	power					901:905	statistical power	889:905	statistical power	889:905	Among the statistical methods used to identify epistatic interactions in genome-wide qualitative association studies (GWAS), gene-based methods have recently grown in popularity due to their better statistical power and biological interpretability.
32564953	0	82	from	epistasis	48:56	arg1	expression					90:99	phenotypic expression	79:99	phenotypic expression of milk yield and composition in Murciano-Granadina goats	79:157	Nonparametric analysis of casein complex genes' epistasis and their effects on phenotypic expression of milk yield and composition in Murciano-Granadina goats.
32564953	9	83	theme	SNP	1905:1907	arg1	groups					1909:1914	7 SNP groups	1903:1914	7 SNP groups	1903:1914	Our results suggest that 79.65% of variability in the traits evaluated may be ascribed to the epistatic relationships across and within 7 SNP groups.
32564953	0	84	theme	milk	104:107	arg1	yield					109:113	milk yield	104:113	milk yield	104:113	Nonparametric analysis of casein complex genes' epistasis and their effects on phenotypic expression of milk yield and composition in Murciano-Granadina goats.
32564953	1	85	theme	genomic	372:378	arg1	research					380:387	genomic research	372:387	genomic research	372:387	Improving knowledge on the causative polymorphisms or genes regulating the expression of milk quantitative and qualitative traits and their interconnections plays a major role in dairy goat breeding programs and genomic research.
32564953	13	86	theme	selective	2570:2578	arg1	practices					2580:2588	selective practices	2570:2588	selective practices for economically important dairy traits	2570:2628	Hence, studying epistasis in isolation may be crucial to optimize selective practices for economically important dairy traits.
32564953	11	87	theme	loading	2286:2292	arg1	|||0.5|||					2296:2300	|component loading > ||0.5|||	2276:2300	|component loading > ||0.5|||	2276:2300	|Additionally, SNP18, 32, and 36 (CSN1S2, CSN1S1, and CSN2 loci, respectively) were the most significant SNP to explain intragroup epistatic variability (component loading > ||0.5||).|
32564953	11	87	theme	loading	2286:2292	arg1	variability					2263:2273	intragroup epistatic variability	2242:2273	|intragroup epistatic variability (component loading > ||0.5||)|	2242:2301	|Additionally, SNP18, 32, and 36 (CSN1S2, CSN1S1, and CSN2 loci, respectively) were the most significant SNP to explain intragroup epistatic variability (component loading > ||0.5||).|
32564953	10	88	theme	multiple	2102:2109	arg1	%					2099:2099	42.43%	2094:2099	42.43% (multiple fit ≥ 0.1)	2094:2120	Two partially overlapping groups of epistatically interrelated SNP were detected: one group of 21 SNP, explaining 57.56% of variability, and another group of 20 SNP, explaining 42.43% (multiple fit ≥ 0.1).
32564953	10	88	theme	multiple	2102:2109	arg1	≥					2115:2115	multiple fit ≥ 0.1	2102:2119	multiple fit ≥ 0.1	2102:2119	Two partially overlapping groups of epistatically interrelated SNP were detected: one group of 21 SNP, explaining 57.56% of variability, and another group of 20 SNP, explaining 42.43% (multiple fit ≥ 0.1).
32564953	6	89	theme	qualitative	1346:1356	arg1	nature					1358:1363	the qualitative nature	1342:1363	the qualitative nature of the SNP used to quantify the genotypes produced as a result	1342:1426	Thus, the aims of this study were to quantify the epistatic relationships among 48 SNP in the casein complex on the expression of milk yield and components (fat, protein, dry matter, lactose, and somatic cells) in Murciano-Granadina goats, to explain the qualitative nature of the SNP used to quantify the genotypes produced as a result.
32564953	5	90	theme	statistical	1072:1082	arg1	power					1084:1088	statistical power	1072:1088	statistical power	1072:1088	However, most of these methods make strong assumptions about the magnitude of the relationships between SNP and phenotype, limiting statistical power.
32564953	0	91	theme	casein	26:31	arg1	genes					41:45	casein complex genes	26:45	casein complex genes' epistasis and their effects on phenotypic expression of milk yield and composition in Murciano-Granadina goats	26:157	Nonparametric analysis of casein complex genes' epistasis and their effects on phenotypic expression of milk yield and composition in Murciano-Granadina goats.
32564953	2	92	theme	tools	456:460	arg1	optimization					415:426	optimization	415:426	optimization of predictive and selective tools	415:460	This information enables optimization of predictive and selective tools, to obtain better-performing animals to help satisfy market demands more efficiently.
32564953	0	93	theme	genes	41:45	arg1	analysis					14:21	Nonparametric analysis	0:21	Nonparametric analysis of casein complex genes' epistasis and their effects on phenotypic expression of milk yield and composition in Murciano-Granadina goats.	0:158	Nonparametric analysis of casein complex genes' epistasis and their effects on phenotypic expression of milk yield and composition in Murciano-Granadina goats.
32564953	7	94	theme	principal	1441:1449	arg1	CATPCA					1471:1476	CATPCA	1471:1476	CATPCA	1471:1476	Categorical principal component analysis (CATPCA) was used to delimit and group the number of SNP studied depending on their implications in the explanation of milk yield and components variability.
32564953	7	94	theme	principal	1441:1449	arg1	analysis					1461:1468	Categorical principal component analysis	1429:1468	Categorical principal component analysis (CATPCA)	1429:1477	Categorical principal component analysis (CATPCA) was used to delimit and group the number of SNP studied depending on their implications in the explanation of milk yield and components variability.
32564953	6	95	theme	Murciano-Granadina	1305:1322	arg1	goats					1324:1328	Murciano-Granadina goats	1305:1328	Murciano-Granadina goats	1305:1328	Thus, the aims of this study were to quantify the epistatic relationships among 48 SNP in the casein complex on the expression of milk yield and components (fat, protein, dry matter, lactose, and somatic cells) in Murciano-Granadina goats, to explain the qualitative nature of the SNP used to quantify the genotypes produced as a result.
32564953	1	96	theme	major	325:329	arg1	role					331:334	a major role	323:334	a major role	323:334	Improving knowledge on the causative polymorphisms or genes regulating the expression of milk quantitative and qualitative traits and their interconnections plays a major role in dairy goat breeding programs and genomic research.
32564953	0	97	dep	genes	41:45	arg1	effects					68:74	their effects	62:74	their effects on phenotypic expression of milk yield and composition in Murciano-Granadina goats	62:157	Nonparametric analysis of casein complex genes' epistasis and their effects on phenotypic expression of milk yield and composition in Murciano-Granadina goats.
32564953	0	97	dep	genes	41:45	arg1	epistasis					48:56	epistasis	48:56	epistasis	48:56	Nonparametric analysis of casein complex genes' epistasis and their effects on phenotypic expression of milk yield and composition in Murciano-Granadina goats.
32564953	2	98	theme	better-performing	473:489	arg1	animals					491:497	better-performing animals	473:497	better-performing animals	473:497	This information enables optimization of predictive and selective tools, to obtain better-performing animals to help satisfy market demands more efficiently.
32564953	4	99	theme	qualitative	776:786	arg1	GWAS					809:812	GWAS	809:812	GWAS	809:812	Among the statistical methods used to identify epistatic interactions in genome-wide qualitative association studies (GWAS), gene-based methods have recently grown in popularity due to their better statistical power and biological interpretability.
32564953	4	99	theme	qualitative	776:786	arg1	studies					800:806	genome-wide qualitative association studies	764:806	genome-wide qualitative association studies (GWAS)	764:813	Among the statistical methods used to identify epistatic interactions in genome-wide qualitative association studies (GWAS), gene-based methods have recently grown in popularity due to their better statistical power and biological interpretability.
32564953	12	100	dep	may	2341:2343	arg1	only					2349:2352	only	2349:2352	only	2349:2352	Conclusively, milk yield and quality may not only depend on the specific casein gene pool of individuals, but may also be relevantly conditioned by the relationships set across and within such genes.
32564953	13	101	theme	dairy	2617:2621	arg1	traits					2623:2628	economically important dairy traits	2594:2628	economically important dairy traits	2594:2628	Hence, studying epistasis in isolation may be crucial to optimize selective practices for economically important dairy traits.
32564953	6	102	from	expression	1207:1216	arg1	goats					1324:1328	Murciano-Granadina goats	1305:1328	Murciano-Granadina goats	1305:1328	Thus, the aims of this study were to quantify the epistatic relationships among 48 SNP in the casein complex on the expression of milk yield and components (fat, protein, dry matter, lactose, and somatic cells) in Murciano-Granadina goats, to explain the qualitative nature of the SNP used to quantify the genotypes produced as a result.
32564953	12	103	theme	gene	2384:2387	arg1	pool					2389:2392	the specific casein gene pool	2364:2392	the specific casein gene pool of individuals	2364:2407	Conclusively, milk yield and quality may not only depend on the specific casein gene pool of individuals, but may also be relevantly conditioned by the relationships set across and within such genes.
32564953	4	104	theme	epistatic	738:746	arg1	interactions					748:759	epistatic interactions	738:759	epistatic interactions in genome-wide qualitative association studies (GWAS)	738:813	Among the statistical methods used to identify epistatic interactions in genome-wide qualitative association studies (GWAS), gene-based methods have recently grown in popularity due to their better statistical power and biological interpretability.
32564953	3	105	theme	milk	553:556	arg1	αS2					580:582	αS2	580:582	αS2	580:582	Goat milk casein proteins (αS1, αS2, β, and κ) are encoded by 4 loci (CSN1S1, CSN1S2, CSN2, and CSN3) clustered within 250 kb on chromosome 6.
32564953	3	105	theme	milk	553:556	arg1	αS1					575:577	αS1	575:577	αS1	575:577	Goat milk casein proteins (αS1, αS2, β, and κ) are encoded by 4 loci (CSN1S1, CSN1S2, CSN2, and CSN3) clustered within 250 kb on chromosome 6.
32564953	3	105	theme	milk	553:556	arg1	proteins					565:572	Goat milk casein proteins	548:572	Goat milk casein proteins (αS1, αS2, β, and κ)	548:593	Goat milk casein proteins (αS1, αS2, β, and κ) are encoded by 4 loci (CSN1S1, CSN1S2, CSN2, and CSN3) clustered within 250 kb on chromosome 6.
32564953	6	106	theme	SNP	1372:1374	arg1	nature					1358:1363	the qualitative nature	1342:1363	the qualitative nature of the SNP used to quantify the genotypes produced as a result	1342:1426	Thus, the aims of this study were to quantify the epistatic relationships among 48 SNP in the casein complex on the expression of milk yield and components (fat, protein, dry matter, lactose, and somatic cells) in Murciano-Granadina goats, to explain the qualitative nature of the SNP used to quantify the genotypes produced as a result.
32564953	1	107	theme	milk	249:252	arg1	traits					283:288	milk quantitative and qualitative traits	249:288	milk quantitative and qualitative traits	249:288	Improving knowledge on the causative polymorphisms or genes regulating the expression of milk quantitative and qualitative traits and their interconnections plays a major role in dairy goat breeding programs and genomic research.
32522696	6	0	theme	CG	837:838	arg1	group					840:844	the CG group	833:844	the CG group	833:844	Comparison of the B group with the CG group revealed 732 upregulated and 510 downregulated genes.
32522696	12	1	theme	plant	1697:1701	arg1	process					1711:1717	the plant disease process	1693:1717	the plant disease process	1693:1717	The chitinase gene was related to the plant disease process.
32522696	4	2	theme	leaf	557:560	arg1	genes					562:566	papaya leaf genes	550:566	papaya leaf genes	550:566	Here, the transcriptional map of papaya leaf genes were subjected to three treatments, viz., non-viral inoculation without CTS-N treatment (CK), virus inoculation without CTS-N treatment (CG), and virus inoculation of 1 g/L treatment (B).
32522696	13	3	theme	enhanced	1878:1885	arg1	resistance					1894:1903	enhanced immune resistance	1878:1903	enhanced immune resistance in papaya	1878:1913	Related genes in plant hormone signal transduction pathways are associated with plant resistance, and six differentially expressed genes were correlated with enhanced immune resistance in papaya.
32522696	2	4	theme	immunomodulatory	318:333	arg1	response					335:342	an immunomodulatory response	315:342	an immunomodulatory response in papaya against Papaya leaf-distortion mosaic virus (PLDMV)	315:404	CTS-N was found to induce an immunomodulatory response in papaya against Papaya leaf-distortion mosaic virus (PLDMV).
32522696	12	5	theme	chitinase	1663:1671	arg1	gene					1673:1676	The chitinase gene	1659:1676	The chitinase gene	1659:1676	The chitinase gene was related to the plant disease process.
32522696	12	5	theme	chitinase	1663:1671	arg1	related					1682:1688	related	1682:1688	related	1682:1688	The chitinase gene was related to the plant disease process.
32522696	9	6	theme	starch	1324:1329	arg1	metabolism					1343:1352	starch and sucrose metabolism	1324:1352	metabolism	1343:1352	Kyoto Encyclopedia of Genes and Genomes (KEGG) results revealed that the top three pathways were phenylpropane biosynthesis, starch and sucrose metabolism, and plant hormone signal transduction.
32522696	6	7	theme	B	820:820	arg1	group					822:826	the B group	816:826	the B group	816:826	Comparison of the B group with the CG group revealed 732 upregulated and 510 downregulated genes.
32522696	10	8	with	consistent	1441:1450	arg1	coefficient					1503:1513	a correlation coefficient	1489:1513	a correlation coefficient of 0.87	1489:1521	Real-time Quantitative PCR (qPCR) results were consistent with the transcriptome results, with a correlation coefficient of 0.87.
32522696	10	8	with	consistent	1441:1450	arg1	results					1475:1481	the transcriptome results	1457:1481	the transcriptome results	1457:1481	Real-time Quantitative PCR (qPCR) results were consistent with the transcriptome results, with a correlation coefficient of 0.87.
32522696	11	9	theme	group	1559:1563	arg1	results					1528:1534	The results	1524:1534	The results of the transcriptional group	1524:1563	The results of the transcriptional group showed that genes associated with plant resistance were induced by CTS-N-treatment in papaya.
32522696	9	10	theme	sucrose	1335:1341	arg1	metabolism					1343:1352	starch and sucrose metabolism	1324:1352	metabolism	1343:1352	Kyoto Encyclopedia of Genes and Genomes (KEGG) results revealed that the top three pathways were phenylpropane biosynthesis, starch and sucrose metabolism, and plant hormone signal transduction.
32522696	8	11	theme	biological	1081:1090	arg1	genes					1100:1104	480 biological process genes	1077:1104	480 biological process genes	1077:1104	To determine gene function, gene ontology (GO) analysis was performed, where 480 biological process genes, 256 molecular function genes, and 343 cell composition genes were differentially expressed.
32522696	13	12	theme	Related	1720:1726	arg1	genes					1728:1732	Related genes	1720:1732	Related genes in plant hormone signal transduction pathways	1720:1778	Related genes in plant hormone signal transduction pathways are associated with plant resistance, and six differentially expressed genes were correlated with enhanced immune resistance in papaya.
32522696	8	13	theme	343	1141:1143	arg1	genes					1162:1166	343 cell composition genes	1141:1166	343 cell composition genes	1141:1166	To determine gene function, gene ontology (GO) analysis was performed, where 480 biological process genes, 256 molecular function genes, and 343 cell composition genes were differentially expressed.
32522696	10	14	theme	correlation	1491:1501	arg1	coefficient					1503:1513	a correlation coefficient	1489:1513	a correlation coefficient of 0.87	1489:1521	Real-time Quantitative PCR (qPCR) results were consistent with the transcriptome results, with a correlation coefficient of 0.87.
32522696	1	15	theme	plants	164:169	arg1	regulation					126:135	Plant immune regulation	113:135	Plant immune regulation	113:135	Plant immune regulation is a defensive strategy of plants for protection against pathogen invasion, and Chitosan-N (CTS-N) can induce plant autoimmunity regulation mechanisms.
32522696	1	15	theme	plants	164:169	arg1	strategy					152:159	a defensive strategy	140:159	a defensive strategy of plants for protection against pathogen invasion	140:210	Plant immune regulation is a defensive strategy of plants for protection against pathogen invasion, and Chitosan-N (CTS-N) can induce plant autoimmunity regulation mechanisms.
32522696	8	16	theme	composition	1150:1160	arg1	genes					1162:1166	343 cell composition genes	1141:1166	343 cell composition genes	1141:1166	To determine gene function, gene ontology (GO) analysis was performed, where 480 biological process genes, 256 molecular function genes, and 343 cell composition genes were differentially expressed.
32522696	1	17	theme	pathogen	194:201	arg1	invasion					203:210	pathogen invasion	194:210	pathogen invasion	194:210	Plant immune regulation is a defensive strategy of plants for protection against pathogen invasion, and Chitosan-N (CTS-N) can induce plant autoimmunity regulation mechanisms.
32522696	9	18	theme	plant	1359:1363	arg1	transduction					1380:1391	plant hormone signal transduction	1359:1391	plant hormone signal transduction	1359:1391	Kyoto Encyclopedia of Genes and Genomes (KEGG) results revealed that the top three pathways were phenylpropane biosynthesis, starch and sucrose metabolism, and plant hormone signal transduction.
32522696	4	19	theme	non-viral	610:618	arg1	inoculation					620:630	non-viral inoculation	610:630	non-viral inoculation without CTS-N treatment (CK)	610:659	Here, the transcriptional map of papaya leaf genes were subjected to three treatments, viz., non-viral inoculation without CTS-N treatment (CK), virus inoculation without CTS-N treatment (CG), and virus inoculation of 1 g/L treatment (B).
32522696	4	19	theme	non-viral	610:618	arg1	treatments					592:601	three treatments	586:601	three treatments	586:601	Here, the transcriptional map of papaya leaf genes were subjected to three treatments, viz., non-viral inoculation without CTS-N treatment (CK), virus inoculation without CTS-N treatment (CG), and virus inoculation of 1 g/L treatment (B).
32522696	5	20	theme	pot	778:780	arg1	experiment					790:799	pot culture experiment	778:799	pot culture experiment	778:799	These were studied by pot culture experiment.
32522696	8	21	theme	function	1121:1128	arg1	genes					1130:1134	256 molecular function genes	1107:1134	256 molecular function genes	1107:1134	To determine gene function, gene ontology (GO) analysis was performed, where 480 biological process genes, 256 molecular function genes, and 343 cell composition genes were differentially expressed.
32522696	6	22	theme	upregulated	859:869	arg1	genes					893:897	732 upregulated and 510 downregulated genes	855:897	732 upregulated and 510 downregulated genes	855:897	Comparison of the B group with the CG group revealed 732 upregulated and 510 downregulated genes.
32522696	7	23	theme	upregulated	958:968	arg1	genes					993:997	909 upregulated and 1024 downregulated genes	954:997	909 upregulated and 1024 downregulated genes	954:997	Comparison of the CG group with the CK group revealed 909 upregulated and 1024 downregulated genes.
32522696	7	24	theme	group	921:925	arg1	Comparison					900:909	Comparison	900:909	Comparison of the CG group with the CK group	900:943	Comparison of the CG group with the CK group revealed 909 upregulated and 1024 downregulated genes.
32522696	4	25	theme	CTS-N	688:692	arg1	CG					705:706	CG	705:706	CG	705:706	Here, the transcriptional map of papaya leaf genes were subjected to three treatments, viz., non-viral inoculation without CTS-N treatment (CK), virus inoculation without CTS-N treatment (CG), and virus inoculation of 1 g/L treatment (B).
32522696	4	25	theme	CTS-N	688:692	arg1	treatment					694:702	CTS-N treatment	688:702	CTS-N treatment (CG)	688:707	Here, the transcriptional map of papaya leaf genes were subjected to three treatments, viz., non-viral inoculation without CTS-N treatment (CK), virus inoculation without CTS-N treatment (CG), and virus inoculation of 1 g/L treatment (B).
32522696	1	26	theme	Plant	113:117	arg1	regulation					126:135	Plant immune regulation	113:135	Plant immune regulation	113:135	Plant immune regulation is a defensive strategy of plants for protection against pathogen invasion, and Chitosan-N (CTS-N) can induce plant autoimmunity regulation mechanisms.
32522696	1	26	theme	Plant	113:117	arg1	strategy					152:159	a defensive strategy	140:159	a defensive strategy of plants for protection against pathogen invasion	140:210	Plant immune regulation is a defensive strategy of plants for protection against pathogen invasion, and Chitosan-N (CTS-N) can induce plant autoimmunity regulation mechanisms.
32522696	13	27	theme	expressed	1841:1849	arg1	genes					1851:1855	six differentially expressed genes	1822:1855	six differentially expressed genes	1822:1855	Related genes in plant hormone signal transduction pathways are associated with plant resistance, and six differentially expressed genes were correlated with enhanced immune resistance in papaya.
32522696	13	28	theme	plant	1800:1804	arg1	resistance					1806:1815	plant resistance	1800:1815	plant resistance	1800:1815	Related genes in plant hormone signal transduction pathways are associated with plant resistance, and six differentially expressed genes were correlated with enhanced immune resistance in papaya.
32522696	0	29	theme	immune	55:60	arg1	L.					51:52	Carica papaya L.	37:52	Carica papaya L.	37:52	Gene expression profiling of papaya (Carica papaya L.) immune response induced by CTS-N after inoculating PLDMV.
32522696	0	29	theme	immune	55:60	arg1	response					62:69	papaya (Carica papaya L.) immune response	29:69	papaya (Carica papaya L.) immune response	29:69	Gene expression profiling of papaya (Carica papaya L.) immune response induced by CTS-N after inoculating PLDMV.
32522696	2	30	theme	mosaic	385:390	arg1	PLDMV					399:403	PLDMV	399:403	PLDMV	399:403	CTS-N was found to induce an immunomodulatory response in papaya against Papaya leaf-distortion mosaic virus (PLDMV).
32522696	2	30	theme	mosaic	385:390	arg1	virus					392:396	Papaya leaf-distortion mosaic virus	362:396	Papaya leaf-distortion mosaic virus (PLDMV)	362:404	CTS-N was found to induce an immunomodulatory response in papaya against Papaya leaf-distortion mosaic virus (PLDMV).
32522696	2	31	theme	Papaya	362:367	arg1	PLDMV					399:403	PLDMV	399:403	PLDMV	399:403	CTS-N was found to induce an immunomodulatory response in papaya against Papaya leaf-distortion mosaic virus (PLDMV).
32522696	2	31	theme	Papaya	362:367	arg1	virus					392:396	Papaya leaf-distortion mosaic virus	362:396	Papaya leaf-distortion mosaic virus (PLDMV)	362:404	CTS-N was found to induce an immunomodulatory response in papaya against Papaya leaf-distortion mosaic virus (PLDMV).
32522696	0	32	theme	Gene	0:3	arg1	profiling					16:24	Gene expression profiling	0:24	Gene expression profiling of papaya (Carica papaya L.) immune response	0:69	Gene expression profiling of papaya (Carica papaya L.) immune response induced by CTS-N after inoculating PLDMV.
32522696	10	33	theme	Quantitative	1404:1415	arg1	qPCR					1422:1425	qPCR	1422:1425	qPCR	1422:1425	Real-time Quantitative PCR (qPCR) results were consistent with the transcriptome results, with a correlation coefficient of 0.87.
32522696	10	33	theme	Quantitative	1404:1415	arg1	PCR					1417:1419	Real-time Quantitative PCR	1394:1419	Real-time Quantitative PCR (qPCR) results	1394:1434	Real-time Quantitative PCR (qPCR) results were consistent with the transcriptome results, with a correlation coefficient of 0.87.
32522696	13	34	theme	plant	1737:1741	arg1	pathways					1771:1778	plant hormone signal transduction pathways	1737:1778	plant hormone signal transduction pathways	1737:1778	Related genes in plant hormone signal transduction pathways are associated with plant resistance, and six differentially expressed genes were correlated with enhanced immune resistance in papaya.
32522696	3	35	theme	expression	425:434	arg1	profile					436:442	the gene expression profile	416:442	the gene expression profile of CTS-N-induced papaya immunomodulatory response	416:492	To date, the gene expression profile of CTS-N-induced papaya immunomodulatory response has not been reported.
32522696	1	36	theme	autoimmunity	253:264	arg1	mechanisms					277:286	plant autoimmunity regulation mechanisms	247:286	plant autoimmunity regulation mechanisms	247:286	Plant immune regulation is a defensive strategy of plants for protection against pathogen invasion, and Chitosan-N (CTS-N) can induce plant autoimmunity regulation mechanisms.
32522696	13	37	from	resistance	1894:1903	arg1	papaya					1908:1913	papaya	1908:1913	papaya	1908:1913	Related genes in plant hormone signal transduction pathways are associated with plant resistance, and six differentially expressed genes were correlated with enhanced immune resistance in papaya.
32522696	13	38	theme	signal	1751:1756	arg1	pathways					1771:1778	plant hormone signal transduction pathways	1737:1778	plant hormone signal transduction pathways	1737:1778	Related genes in plant hormone signal transduction pathways are associated with plant resistance, and six differentially expressed genes were correlated with enhanced immune resistance in papaya.
32522696	5	39	theme	culture	782:788	arg1	experiment					790:799	pot culture experiment	778:799	pot culture experiment	778:799	These were studied by pot culture experiment.
32522696	0	40	theme	papaya	29:34	arg1	L.					51:52	Carica papaya L.	37:52	Carica papaya L.	37:52	Gene expression profiling of papaya (Carica papaya L.) immune response induced by CTS-N after inoculating PLDMV.
32522696	0	40	theme	papaya	29:34	arg1	response					62:69	papaya (Carica papaya L.) immune response	29:69	papaya (Carica papaya L.) immune response	29:69	Gene expression profiling of papaya (Carica papaya L.) immune response induced by CTS-N after inoculating PLDMV.
32522696	3	41	theme	papaya	461:466	arg1	response					485:492	CTS-N-induced papaya immunomodulatory response	447:492	CTS-N-induced papaya immunomodulatory response	447:492	To date, the gene expression profile of CTS-N-induced papaya immunomodulatory response has not been reported.
32522696	0	42	theme	Carica	37:42	arg1	L.					51:52	Carica papaya L.	37:52	Carica papaya L.	37:52	Gene expression profiling of papaya (Carica papaya L.) immune response induced by CTS-N after inoculating PLDMV.
32522696	0	42	theme	Carica	37:42	arg1	response					62:69	papaya (Carica papaya L.) immune response	29:69	papaya (Carica papaya L.) immune response	29:69	Gene expression profiling of papaya (Carica papaya L.) immune response induced by CTS-N after inoculating PLDMV.
32522696	4	43	theme	1 g/L	735:739	arg1	treatment					741:749	1 g/L treatment	735:749	1 g/L treatment (B)	735:753	Here, the transcriptional map of papaya leaf genes were subjected to three treatments, viz., non-viral inoculation without CTS-N treatment (CK), virus inoculation without CTS-N treatment (CG), and virus inoculation of 1 g/L treatment (B).
32522696	4	43	theme	1 g/L	735:739	arg1	B					752:752	B	752:752	B	752:752	Here, the transcriptional map of papaya leaf genes were subjected to three treatments, viz., non-viral inoculation without CTS-N treatment (CK), virus inoculation without CTS-N treatment (CG), and virus inoculation of 1 g/L treatment (B).
32522696	9	44	theme	top	1272:1274	arg1	pathways					1282:1289	the top three pathways	1268:1289	the top three pathways	1268:1289	Kyoto Encyclopedia of Genes and Genomes (KEGG) results revealed that the top three pathways were phenylpropane biosynthesis, starch and sucrose metabolism, and plant hormone signal transduction.
32522696	9	44	theme	top	1272:1274	arg1	biosynthesis					1310:1321	phenylpropane biosynthesis	1296:1321	phenylpropane biosynthesis	1296:1321	Kyoto Encyclopedia of Genes and Genomes (KEGG) results revealed that the top three pathways were phenylpropane biosynthesis, starch and sucrose metabolism, and plant hormone signal transduction.
32522696	8	45	theme	gene	1028:1031	arg1	ontology					1033:1040	gene ontology	1028:1040	gene ontology (GO) analysis	1028:1054	To determine gene function, gene ontology (GO) analysis was performed, where 480 biological process genes, 256 molecular function genes, and 343 cell composition genes were differentially expressed.
32522696	12	46	theme	disease	1703:1709	arg1	process					1711:1717	the plant disease process	1693:1717	the plant disease process	1693:1717	The chitinase gene was related to the plant disease process.
32522696	7	47	dep	downregulated	979:991	arg1	1024					974:977	1024	974:977	1024	974:977	Comparison of the CG group with the CK group revealed 909 upregulated and 1024 downregulated genes.
32522696	11	48	theme	transcriptional	1543:1557	arg1	group					1559:1563	the transcriptional group	1539:1563	the transcriptional group	1539:1563	The results of the transcriptional group showed that genes associated with plant resistance were induced by CTS-N-treatment in papaya.
32522696	3	49	theme	response	485:492	arg1	profile					436:442	the gene expression profile	416:442	the gene expression profile of CTS-N-induced papaya immunomodulatory response	416:492	To date, the gene expression profile of CTS-N-induced papaya immunomodulatory response has not been reported.
32522696	9	50	theme	phenylpropane	1296:1308	arg1	pathways					1282:1289	the top three pathways	1268:1289	the top three pathways	1268:1289	Kyoto Encyclopedia of Genes and Genomes (KEGG) results revealed that the top three pathways were phenylpropane biosynthesis, starch and sucrose metabolism, and plant hormone signal transduction.
32522696	9	50	theme	phenylpropane	1296:1308	arg1	biosynthesis					1310:1321	phenylpropane biosynthesis	1296:1321	phenylpropane biosynthesis	1296:1321	Kyoto Encyclopedia of Genes and Genomes (KEGG) results revealed that the top three pathways were phenylpropane biosynthesis, starch and sucrose metabolism, and plant hormone signal transduction.
32522696	4	51	theme	papaya	550:555	arg1	genes					562:566	papaya leaf genes	550:566	papaya leaf genes	550:566	Here, the transcriptional map of papaya leaf genes were subjected to three treatments, viz., non-viral inoculation without CTS-N treatment (CK), virus inoculation without CTS-N treatment (CG), and virus inoculation of 1 g/L treatment (B).
32522696	13	52	theme	immune	1887:1892	arg1	resistance					1894:1903	enhanced immune resistance	1878:1903	enhanced immune resistance in papaya	1878:1913	Related genes in plant hormone signal transduction pathways are associated with plant resistance, and six differentially expressed genes were correlated with enhanced immune resistance in papaya.
32522696	6	53	theme	group	822:826	arg1	Comparison					802:811	Comparison	802:811	Comparison of the B group with the CG group	802:844	Comparison of the B group with the CG group revealed 732 upregulated and 510 downregulated genes.
32522696	4	54	theme	genes	562:566	arg1	map					543:545	the transcriptional map	523:545	the transcriptional map of papaya leaf genes	523:566	Here, the transcriptional map of papaya leaf genes were subjected to three treatments, viz., non-viral inoculation without CTS-N treatment (CK), virus inoculation without CTS-N treatment (CG), and virus inoculation of 1 g/L treatment (B).
32522696	10	55	theme	transcriptome	1461:1473	arg1	results					1475:1481	the transcriptome results	1457:1481	the transcriptome results	1457:1481	Real-time Quantitative PCR (qPCR) results were consistent with the transcriptome results, with a correlation coefficient of 0.87.
32522696	1	56	theme	defensive	142:150	arg1	regulation					126:135	Plant immune regulation	113:135	Plant immune regulation	113:135	Plant immune regulation is a defensive strategy of plants for protection against pathogen invasion, and Chitosan-N (CTS-N) can induce plant autoimmunity regulation mechanisms.
32522696	1	56	theme	defensive	142:150	arg1	strategy					152:159	a defensive strategy	140:159	a defensive strategy of plants for protection against pathogen invasion	140:210	Plant immune regulation is a defensive strategy of plants for protection against pathogen invasion, and Chitosan-N (CTS-N) can induce plant autoimmunity regulation mechanisms.
32522696	2	57	from	response	335:342	arg1	papaya					347:352	papaya	347:352	papaya	347:352	CTS-N was found to induce an immunomodulatory response in papaya against Papaya leaf-distortion mosaic virus (PLDMV).
32522696	8	58	dep	ontology	1033:1040	arg1	GO					1043:1044	GO	1043:1044	GO	1043:1044	To determine gene function, gene ontology (GO) analysis was performed, where 480 biological process genes, 256 molecular function genes, and 343 cell composition genes were differentially expressed.
32522696	4	59	theme	CTS-N	640:644	arg1	CK					657:658	CK	657:658	CK	657:658	Here, the transcriptional map of papaya leaf genes were subjected to three treatments, viz., non-viral inoculation without CTS-N treatment (CK), virus inoculation without CTS-N treatment (CG), and virus inoculation of 1 g/L treatment (B).
32522696	4	59	theme	CTS-N	640:644	arg1	treatment					646:654	CTS-N treatment	640:654	CTS-N treatment (CK)	640:659	Here, the transcriptional map of papaya leaf genes were subjected to three treatments, viz., non-viral inoculation without CTS-N treatment (CK), virus inoculation without CTS-N treatment (CG), and virus inoculation of 1 g/L treatment (B).
32522696	7	60	theme	downregulated	979:991	arg1	genes					993:997	909 upregulated and 1024 downregulated genes	954:997	909 upregulated and 1024 downregulated genes	954:997	Comparison of the CG group with the CK group revealed 909 upregulated and 1024 downregulated genes.
32522696	8	61	theme	process	1092:1098	arg1	genes					1100:1104	480 biological process genes	1077:1104	480 biological process genes	1077:1104	To determine gene function, gene ontology (GO) analysis was performed, where 480 biological process genes, 256 molecular function genes, and 343 cell composition genes were differentially expressed.
32522696	6	62	theme	downregulated	879:891	arg1	genes					893:897	732 upregulated and 510 downregulated genes	855:897	732 upregulated and 510 downregulated genes	855:897	Comparison of the B group with the CG group revealed 732 upregulated and 510 downregulated genes.
32522696	8	63	theme	cell	1145:1148	arg1	genes					1162:1166	343 cell composition genes	1141:1166	343 cell composition genes	1141:1166	To determine gene function, gene ontology (GO) analysis was performed, where 480 biological process genes, 256 molecular function genes, and 343 cell composition genes were differentially expressed.
32522696	9	64	theme	hormone	1365:1371	arg1	transduction					1380:1391	plant hormone signal transduction	1359:1391	plant hormone signal transduction	1359:1391	Kyoto Encyclopedia of Genes and Genomes (KEGG) results revealed that the top three pathways were phenylpropane biosynthesis, starch and sucrose metabolism, and plant hormone signal transduction.
32522696	10	65	theme	0.87	1518:1521	arg1	coefficient					1503:1513	a correlation coefficient	1489:1513	a correlation coefficient of 0.87	1489:1521	Real-time Quantitative PCR (qPCR) results were consistent with the transcriptome results, with a correlation coefficient of 0.87.
32522696	7	66	theme	CK	936:937	arg1	group					939:943	the CK group	932:943	the CK group	932:943	Comparison of the CG group with the CK group revealed 909 upregulated and 1024 downregulated genes.
32522696	8	67	theme	molecular	1111:1119	arg1	genes					1130:1134	256 molecular function genes	1107:1134	256 molecular function genes	1107:1134	To determine gene function, gene ontology (GO) analysis was performed, where 480 biological process genes, 256 molecular function genes, and 343 cell composition genes were differentially expressed.
32522696	6	68	dep	downregulated	879:891	arg1	510					875:877	510	875:877	510	875:877	Comparison of the B group with the CG group revealed 732 upregulated and 510 downregulated genes.
32522696	4	69	theme	virus	662:666	arg1	inoculation					668:678	virus inoculation	662:678	virus inoculation without CTS-N treatment (CG)	662:707	Here, the transcriptional map of papaya leaf genes were subjected to three treatments, viz., non-viral inoculation without CTS-N treatment (CK), virus inoculation without CTS-N treatment (CG), and virus inoculation of 1 g/L treatment (B).
32522696	4	69	theme	virus	662:666	arg1	treatments					592:601	three treatments	586:601	three treatments	586:601	Here, the transcriptional map of papaya leaf genes were subjected to three treatments, viz., non-viral inoculation without CTS-N treatment (CK), virus inoculation without CTS-N treatment (CG), and virus inoculation of 1 g/L treatment (B).
32522696	13	70	from	genes	1728:1732	arg1	pathways					1771:1778	plant hormone signal transduction pathways	1737:1778	plant hormone signal transduction pathways	1737:1778	Related genes in plant hormone signal transduction pathways are associated with plant resistance, and six differentially expressed genes were correlated with enhanced immune resistance in papaya.
32522696	7	71	with	Comparison	900:909	arg1	group					939:943	the CK group	932:943	the CK group	932:943	Comparison of the CG group with the CK group revealed 909 upregulated and 1024 downregulated genes.
32522696	7	72	theme	CG	918:919	arg1	group					921:925	the CG group	914:925	the CG group	914:925	Comparison of the CG group with the CK group revealed 909 upregulated and 1024 downregulated genes.
32522696	1	73	theme	immune	119:124	arg1	regulation					126:135	Plant immune regulation	113:135	Plant immune regulation	113:135	Plant immune regulation is a defensive strategy of plants for protection against pathogen invasion, and Chitosan-N (CTS-N) can induce plant autoimmunity regulation mechanisms.
32522696	1	73	theme	immune	119:124	arg1	strategy					152:159	a defensive strategy	140:159	a defensive strategy of plants for protection against pathogen invasion	140:210	Plant immune regulation is a defensive strategy of plants for protection against pathogen invasion, and Chitosan-N (CTS-N) can induce plant autoimmunity regulation mechanisms.
32522696	0	74	theme	response	62:69	arg1	profiling					16:24	Gene expression profiling	0:24	Gene expression profiling of papaya (Carica papaya L.) immune response	0:69	Gene expression profiling of papaya (Carica papaya L.) immune response induced by CTS-N after inoculating PLDMV.
32522696	13	75	theme	transduction	1758:1769	arg1	pathways					1771:1778	plant hormone signal transduction pathways	1737:1778	plant hormone signal transduction pathways	1737:1778	Related genes in plant hormone signal transduction pathways are associated with plant resistance, and six differentially expressed genes were correlated with enhanced immune resistance in papaya.
32522696	9	76	dep	results	1246:1252	arg1	revealed					1254:1261	revealed	1254:1261	results revealed that the top three pathways were phenylpropane biosynthesis, starch and sucrose metabolism, and plant hormone signal transduction	1246:1391	Kyoto Encyclopedia of Genes and Genomes (KEGG) results revealed that the top three pathways were phenylpropane biosynthesis, starch and sucrose metabolism, and plant hormone signal transduction.
32522696	4	77	theme	virus	714:718	arg1	treatments					592:601	three treatments	586:601	three treatments	586:601	Here, the transcriptional map of papaya leaf genes were subjected to three treatments, viz., non-viral inoculation without CTS-N treatment (CK), virus inoculation without CTS-N treatment (CG), and virus inoculation of 1 g/L treatment (B).
32522696	4	77	theme	virus	714:718	arg1	inoculation					720:730	virus inoculation	714:730	virus inoculation of 1 g/L treatment (B)	714:753	Here, the transcriptional map of papaya leaf genes were subjected to three treatments, viz., non-viral inoculation without CTS-N treatment (CK), virus inoculation without CTS-N treatment (CG), and virus inoculation of 1 g/L treatment (B).
32522696	2	78	theme	leaf-distortion	369:383	arg1	PLDMV					399:403	PLDMV	399:403	PLDMV	399:403	CTS-N was found to induce an immunomodulatory response in papaya against Papaya leaf-distortion mosaic virus (PLDMV).
32522696	2	78	theme	leaf-distortion	369:383	arg1	virus					392:396	Papaya leaf-distortion mosaic virus	362:396	Papaya leaf-distortion mosaic virus (PLDMV)	362:404	CTS-N was found to induce an immunomodulatory response in papaya against Papaya leaf-distortion mosaic virus (PLDMV).
32522696	0	79	theme	expression	5:14	arg1	profiling					16:24	Gene expression profiling	0:24	Gene expression profiling of papaya (Carica papaya L.) immune response	0:69	Gene expression profiling of papaya (Carica papaya L.) immune response induced by CTS-N after inoculating PLDMV.
32522696	6	80	with	Comparison	802:811	arg1	group					840:844	the CG group	833:844	the CG group	833:844	Comparison of the B group with the CG group revealed 732 upregulated and 510 downregulated genes.
32522696	4	81	theme	transcriptional	527:541	arg1	map					543:545	the transcriptional map	523:545	the transcriptional map of papaya leaf genes	523:566	Here, the transcriptional map of papaya leaf genes were subjected to three treatments, viz., non-viral inoculation without CTS-N treatment (CK), virus inoculation without CTS-N treatment (CG), and virus inoculation of 1 g/L treatment (B).
32522696	1	82	theme	plant	247:251	arg1	mechanisms					277:286	plant autoimmunity regulation mechanisms	247:286	plant autoimmunity regulation mechanisms	247:286	Plant immune regulation is a defensive strategy of plants for protection against pathogen invasion, and Chitosan-N (CTS-N) can induce plant autoimmunity regulation mechanisms.
32522696	10	83	theme	Real-time	1394:1402	arg1	qPCR					1422:1425	qPCR	1422:1425	qPCR	1422:1425	Real-time Quantitative PCR (qPCR) results were consistent with the transcriptome results, with a correlation coefficient of 0.87.
32522696	10	83	theme	Real-time	1394:1402	arg1	PCR					1417:1419	Real-time Quantitative PCR	1394:1419	Real-time Quantitative PCR (qPCR) results	1394:1434	Real-time Quantitative PCR (qPCR) results were consistent with the transcriptome results, with a correlation coefficient of 0.87.
32522696	11	84	theme	plant	1599:1603	arg1	resistance					1605:1614	plant resistance	1599:1614	plant resistance	1599:1614	The results of the transcriptional group showed that genes associated with plant resistance were induced by CTS-N-treatment in papaya.
32522696	13	85	theme	hormone	1743:1749	arg1	pathways					1771:1778	plant hormone signal transduction pathways	1737:1778	plant hormone signal transduction pathways	1737:1778	Related genes in plant hormone signal transduction pathways are associated with plant resistance, and six differentially expressed genes were correlated with enhanced immune resistance in papaya.
32522696	1	86	theme	regulation	266:275	arg1	mechanisms					277:286	plant autoimmunity regulation mechanisms	247:286	plant autoimmunity regulation mechanisms	247:286	Plant immune regulation is a defensive strategy of plants for protection against pathogen invasion, and Chitosan-N (CTS-N) can induce plant autoimmunity regulation mechanisms.
32522696	8	87	theme	gene	1013:1016	arg1	function					1018:1025	gene function	1013:1025	gene function	1013:1025	To determine gene function, gene ontology (GO) analysis was performed, where 480 biological process genes, 256 molecular function genes, and 343 cell composition genes were differentially expressed.
32522696	3	88	theme	gene	420:423	arg1	profile					436:442	the gene expression profile	416:442	the gene expression profile of CTS-N-induced papaya immunomodulatory response	416:492	To date, the gene expression profile of CTS-N-induced papaya immunomodulatory response has not been reported.
32522696	0	89	theme	papaya	44:49	arg1	L.					51:52	Carica papaya L.	37:52	Carica papaya L.	37:52	Gene expression profiling of papaya (Carica papaya L.) immune response induced by CTS-N after inoculating PLDMV.
32522696	0	89	theme	papaya	44:49	arg1	response					62:69	papaya (Carica papaya L.) immune response	29:69	papaya (Carica papaya L.) immune response	29:69	Gene expression profiling of papaya (Carica papaya L.) immune response induced by CTS-N after inoculating PLDMV.
32522696	3	90	theme	immunomodulatory	468:483	arg1	response					485:492	CTS-N-induced papaya immunomodulatory response	447:492	CTS-N-induced papaya immunomodulatory response	447:492	To date, the gene expression profile of CTS-N-induced papaya immunomodulatory response has not been reported.
32522696	8	91	theme	ontology	1033:1040	arg1	analysis					1047:1054	gene ontology (GO) analysis	1028:1054	gene ontology (GO) analysis	1028:1054	To determine gene function, gene ontology (GO) analysis was performed, where 480 biological process genes, 256 molecular function genes, and 343 cell composition genes were differentially expressed.
32522696	10	92	theme	PCR	1417:1419	arg1	results					1428:1434	Real-time Quantitative PCR (qPCR) results	1394:1434	Real-time Quantitative PCR (qPCR) results	1394:1434	Real-time Quantitative PCR (qPCR) results were consistent with the transcriptome results, with a correlation coefficient of 0.87.
32522696	3	93	theme	CTS-N-induced	447:459	arg1	response					485:492	CTS-N-induced papaya immunomodulatory response	447:492	CTS-N-induced papaya immunomodulatory response	447:492	To date, the gene expression profile of CTS-N-induced papaya immunomodulatory response has not been reported.
32522696	9	94	theme	signal	1373:1378	arg1	transduction					1380:1391	plant hormone signal transduction	1359:1391	plant hormone signal transduction	1359:1391	Kyoto Encyclopedia of Genes and Genomes (KEGG) results revealed that the top three pathways were phenylpropane biosynthesis, starch and sucrose metabolism, and plant hormone signal transduction.
32522696	4	95	theme	treatment	741:749	arg1	inoculation					668:678	virus inoculation	662:678	virus inoculation without CTS-N treatment (CG)	662:707	Here, the transcriptional map of papaya leaf genes were subjected to three treatments, viz., non-viral inoculation without CTS-N treatment (CK), virus inoculation without CTS-N treatment (CG), and virus inoculation of 1 g/L treatment (B).
32522696	4	95	theme	treatment	741:749	arg1	treatments					592:601	three treatments	586:601	three treatments	586:601	Here, the transcriptional map of papaya leaf genes were subjected to three treatments, viz., non-viral inoculation without CTS-N treatment (CK), virus inoculation without CTS-N treatment (CG), and virus inoculation of 1 g/L treatment (B).
32522696	4	95	theme	treatment	741:749	arg1	inoculation					620:630	non-viral inoculation	610:630	non-viral inoculation without CTS-N treatment (CK)	610:659	Here, the transcriptional map of papaya leaf genes were subjected to three treatments, viz., non-viral inoculation without CTS-N treatment (CK), virus inoculation without CTS-N treatment (CG), and virus inoculation of 1 g/L treatment (B).
32522696	4	95	theme	treatment	741:749	arg1	inoculation					720:730	virus inoculation	714:730	virus inoculation of 1 g/L treatment (B)	714:753	Here, the transcriptional map of papaya leaf genes were subjected to three treatments, viz., non-viral inoculation without CTS-N treatment (CK), virus inoculation without CTS-N treatment (CG), and virus inoculation of 1 g/L treatment (B).
32788377	2	0	theme	tandem	399:404	arg1	BcsAB					407:411	c-di-GMP-dependent synthase tandem (BcsAB)	371:412	c-di-GMP-dependent synthase tandem (BcsAB)	371:412	Bacterial cellulose is a widespread biofilm component whose secretion in Gram-negative species requires an inner membrane, c-di-GMP-dependent synthase tandem (BcsAB), an outer membrane porin (BcsC), and various accessory subunits that regulate synthase assembly and function as well as the exopolysaccharide's chemical composition and mechanical properties.
32788377	10	1	theme	unexpected	2575:2584	arg1	architecture					2593:2604	unexpected domain architecture	2575:2604	unexpected domain architecture	2575:2604	Crystallographic and functional data reveal that BcsE features unexpected domain architecture and likely acts on multiple levels to fine-tune bacterial cellulose production, from the early stages of secretion system assembly to the maintenence of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.
32788377	3	2	theme	individual	746:755	arg1	components					768:777	individual regulatory components	746:777	individual regulatory components	746:777	We recently showed that in Escherichia coli, most Bcs proteins form a megadalton-sized secretory nanomachine, but the role and structure of individual regulatory components remained enigmatic.
32788377	6	3	theme	third	1305:1309	arg1	BcsENTD					1330:1336	BcsENTD	1330:1336	BcsENTD	1330:1336	In addition, we reveal that a third N-terminal domain (BcsENTD) determines the protein's homooligomerization and targeting of BcsERQ to the membrane as well as previously unreported interactions with transcription antitermination complex components.
32788377	6	3	theme	third	1305:1309	arg1	domain					1322:1327	a third N-terminal domain	1303:1327	a third N-terminal domain (BcsENTD)	1303:1337	In addition, we reveal that a third N-terminal domain (BcsENTD) determines the protein's homooligomerization and targeting of BcsERQ to the membrane as well as previously unreported interactions with transcription antitermination complex components.
32788377	8	4	theme	coli-like	2130:2138	arg1	systems					2154:2160	sophisticated Escherichia coli-like Bcs secretion systems	2104:2160	sophisticated Escherichia coli-like Bcs secretion systems	2104:2160	Whereas its secretion generally involves an inner membrane c-di-GMP-dependent synthase tandem (BcsAB) across the bacterial domain of life, enterobacteria feature sophisticated Escherichia coli-like Bcs secretion systems, where multiple additional subunits are either required for secretion or contribute to the maximal production of the polysaccharide in vivo.
32788377	7	5	theme	microbial	1866:1874	arg1	fitness					1876:1882	microbial fitness	1866:1882	microbial fitness	1866:1882	Together, the data suggest that BcsE acts on multiple levels to fine-tune bacterial cellulose secretion, from the early stages of secretion system assembly to the maintenance of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts.
32788377	8	6	theme	membrane	1992:1999	arg1	BcsAB					2037:2041	an inner membrane c-di-GMP-dependent synthase tandem (BcsAB)	1983:2042	an inner membrane c-di-GMP-dependent synthase tandem (BcsAB) across the bacterial domain of life	1983:2078	Whereas its secretion generally involves an inner membrane c-di-GMP-dependent synthase tandem (BcsAB) across the bacterial domain of life, enterobacteria feature sophisticated Escherichia coli-like Bcs secretion systems, where multiple additional subunits are either required for secretion or contribute to the maximal production of the polysaccharide in vivo.
32788377	10	7	theme	cellulose	2664:2672	arg1	production					2674:2683	fine-tune bacterial cellulose production	2644:2683	fine-tune bacterial cellulose production	2644:2683	Crystallographic and functional data reveal that BcsE features unexpected domain architecture and likely acts on multiple levels to fine-tune bacterial cellulose production, from the early stages of secretion system assembly to the maintenence of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.
32788377	3	8	theme	components	768:777	arg1	role					724:727	role	724:727	role	724:727	We recently showed that in Escherichia coli, most Bcs proteins form a megadalton-sized secretory nanomachine, but the role and structure of individual regulatory components remained enigmatic.
32788377	3	8	theme	components	768:777	arg1	structure					733:741	structure	733:741	structure	733:741	We recently showed that in Escherichia coli, most Bcs proteins form a megadalton-sized secretory nanomachine, but the role and structure of individual regulatory components remained enigmatic.
32788377	8	9	theme	synthase	2020:2027	arg1	BcsAB					2037:2041	an inner membrane c-di-GMP-dependent synthase tandem (BcsAB)	1983:2042	an inner membrane c-di-GMP-dependent synthase tandem (BcsAB) across the bacterial domain of life	1983:2078	Whereas its secretion generally involves an inner membrane c-di-GMP-dependent synthase tandem (BcsAB) across the bacterial domain of life, enterobacteria feature sophisticated Escherichia coli-like Bcs secretion systems, where multiple additional subunits are either required for secretion or contribute to the maximal production of the polysaccharide in vivo.
32788377	5	10	theme	tandem	1124:1129	arg1	domain					1117:1122	a degenerate receiver-GGDEF domain tandem	1089:1129	a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*)	1089:1147	Crystallographic and solution-based data show that BcsE's predicted GIL domain is a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*), where the divergent diguanylate cyclase module binds both dimeric c-di-GMP and BcsQ through mutually independent interfaces.
32788377	5	10	theme	tandem	1124:1129	arg1	domain					1079:1084	BcsE's predicted GIL domain	1058:1084	BcsE's predicted GIL domain	1058:1084	Crystallographic and solution-based data show that BcsE's predicted GIL domain is a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*), where the divergent diguanylate cyclase module binds both dimeric c-di-GMP and BcsQ through mutually independent interfaces.
32788377	5	10	theme	tandem	1124:1129	arg1	*					1146:1146	BcsEREC*-GGDEF*	1132:1146	BcsEREC*-GGDEF*	1132:1146	Crystallographic and solution-based data show that BcsE's predicted GIL domain is a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*), where the divergent diguanylate cyclase module binds both dimeric c-di-GMP and BcsQ through mutually independent interfaces.
32788377	1	11	theme	biofilm-promoting	211:227	arg1	factors					239:245	biofilm-promoting adherence factors	211:245	biofilm-promoting adherence factors	211:245	Most bacteria respond to surfaces by biogenesis of intracellular c-di-GMP, which inhibits motility and induces secretion of biofilm-promoting adherence factors.
32788377	2	12	theme	chemical	558:565	arg1	composition					567:577	the exopolysaccharide's chemical composition	534:577	the exopolysaccharide's chemical composition	534:577	Bacterial cellulose is a widespread biofilm component whose secretion in Gram-negative species requires an inner membrane, c-di-GMP-dependent synthase tandem (BcsAB), an outer membrane porin (BcsC), and various accessory subunits that regulate synthase assembly and function as well as the exopolysaccharide's chemical composition and mechanical properties.
32788377	10	13	theme	fine-tune	2644:2652	arg1	production					2674:2683	fine-tune bacterial cellulose production	2644:2683	fine-tune bacterial cellulose production	2644:2683	Crystallographic and functional data reveal that BcsE features unexpected domain architecture and likely acts on multiple levels to fine-tune bacterial cellulose production, from the early stages of secretion system assembly to the maintenence of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.
32788377	2	14	from	secretion	308:316	arg1	species					335:341	Gram-negative species	321:341	Gram-negative species	321:341	Bacterial cellulose is a widespread biofilm component whose secretion in Gram-negative species requires an inner membrane, c-di-GMP-dependent synthase tandem (BcsAB), an outer membrane porin (BcsC), and various accessory subunits that regulate synthase assembly and function as well as the exopolysaccharide's chemical composition and mechanical properties.
32788377	5	15	theme	BcsEREC	1132:1138	arg1	domain					1117:1122	a degenerate receiver-GGDEF domain tandem	1089:1129	a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*)	1089:1147	Crystallographic and solution-based data show that BcsE's predicted GIL domain is a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*), where the divergent diguanylate cyclase module binds both dimeric c-di-GMP and BcsQ through mutually independent interfaces.
32788377	5	15	theme	BcsEREC	1132:1138	arg1	*					1146:1146	BcsEREC*-GGDEF*	1132:1146	BcsEREC*-GGDEF*	1132:1146	Crystallographic and solution-based data show that BcsE's predicted GIL domain is a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*), where the divergent diguanylate cyclase module binds both dimeric c-di-GMP and BcsQ through mutually independent interfaces.
32788377	1	16	theme	factors	239:245	arg1	secretion					198:206	secretion	198:206	secretion of biofilm-promoting adherence factors	198:245	Most bacteria respond to surfaces by biogenesis of intracellular c-di-GMP, which inhibits motility and induces secretion of biofilm-promoting adherence factors.
32788377	4	17	theme	inner	932:936	arg1	membrane					938:945	the inner membrane	928:945	the inner membrane	928:945	Here, we demonstrate that essential-for-secretion BcsR and BcsQ regulate each other's folding and stability and are recruited to the inner membrane via c-di-GMP-sensing BcsE and its intraoperon partner BcsF.
32788377	7	18	theme	biofilm	1830:1836	arg1	component					1838:1846	a widespread biofilm component	1817:1846	a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts	1817:1939	Together, the data suggest that BcsE acts on multiple levels to fine-tune bacterial cellulose secretion, from the early stages of secretion system assembly to the maintenance of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts.
32788377	7	19	theme	system	1665:1670	arg1	assembly					1672:1679	secretion system assembly	1655:1679	secretion system assembly	1655:1679	Together, the data suggest that BcsE acts on multiple levels to fine-tune bacterial cellulose secretion, from the early stages of secretion system assembly to the maintenance of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts.
32788377	5	20	theme	receiver-GGDEF	1102:1115	arg1	domain					1117:1122	a degenerate receiver-GGDEF domain tandem	1089:1129	a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*)	1089:1147	Crystallographic and solution-based data show that BcsE's predicted GIL domain is a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*), where the divergent diguanylate cyclase module binds both dimeric c-di-GMP and BcsQ through mutually independent interfaces.
32788377	5	20	theme	receiver-GGDEF	1102:1115	arg1	domain					1079:1084	BcsE's predicted GIL domain	1058:1084	BcsE's predicted GIL domain	1058:1084	Crystallographic and solution-based data show that BcsE's predicted GIL domain is a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*), where the divergent diguanylate cyclase module binds both dimeric c-di-GMP and BcsQ through mutually independent interfaces.
32788377	5	20	theme	receiver-GGDEF	1102:1115	arg1	*					1146:1146	BcsEREC*-GGDEF*	1132:1146	BcsEREC*-GGDEF*	1132:1146	Crystallographic and solution-based data show that BcsE's predicted GIL domain is a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*), where the divergent diguanylate cyclase module binds both dimeric c-di-GMP and BcsQ through mutually independent interfaces.
32788377	3	21	theme	most	651:654	arg1	proteins					660:667	most Bcs proteins	651:667	most Bcs proteins	651:667	We recently showed that in Escherichia coli, most Bcs proteins form a megadalton-sized secretory nanomachine, but the role and structure of individual regulatory components remained enigmatic.
32788377	1	22	theme	intracellular	138:150	arg1	c-di-GMP					152:159	intracellular c-di-GMP	138:159	intracellular c-di-GMP	138:159	Most bacteria respond to surfaces by biogenesis of intracellular c-di-GMP, which inhibits motility and induces secretion of biofilm-promoting adherence factors.
32788377	10	23	theme	Crystallographic	2512:2527	arg1	data					2544:2547	Crystallographic and functional data	2512:2547	data	2544:2547	Crystallographic and functional data reveal that BcsE features unexpected domain architecture and likely acts on multiple levels to fine-tune bacterial cellulose production, from the early stages of secretion system assembly to the maintenence of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.
32788377	10	24	theme	secretion	2711:2719	arg1	assembly					2728:2735	secretion system assembly	2711:2735	secretion system assembly	2711:2735	Crystallographic and functional data reveal that BcsE features unexpected domain architecture and likely acts on multiple levels to fine-tune bacterial cellulose production, from the early stages of secretion system assembly to the maintenence of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.
32788377	0	25	theme	Regulator	71:79	arg1	BcsE					81:84	the c-di-GMP-Sensing Cellulose Secretion Regulator BcsE	30:84	the c-di-GMP-Sensing Cellulose Secretion Regulator BcsE	30:84	Structure and Multitasking of the c-di-GMP-Sensing Cellulose Secretion Regulator BcsE.
32788377	7	26	theme	early	1639:1643	arg1	stages					1645:1650	the early stages	1635:1650	the early stages of secretion system assembly	1635:1679	Together, the data suggest that BcsE acts on multiple levels to fine-tune bacterial cellulose secretion, from the early stages of secretion system assembly to the maintenance of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts.
32788377	8	27	theme	life	2075:2078	arg1	life					2075:2078	life	2075:2078	life	2075:2078	Whereas its secretion generally involves an inner membrane c-di-GMP-dependent synthase tandem (BcsAB) across the bacterial domain of life, enterobacteria feature sophisticated Escherichia coli-like Bcs secretion systems, where multiple additional subunits are either required for secretion or contribute to the maximal production of the polysaccharide in vivo.
32788377	8	27	theme	life	2075:2078	arg1	domain					2065:2070	the bacterial domain	2051:2070	the bacterial domain of life	2051:2078	Whereas its secretion generally involves an inner membrane c-di-GMP-dependent synthase tandem (BcsAB) across the bacterial domain of life, enterobacteria feature sophisticated Escherichia coli-like Bcs secretion systems, where multiple additional subunits are either required for secretion or contribute to the maximal production of the polysaccharide in vivo.
32788377	5	28	theme	divergent	1160:1168	arg1	module					1190:1195	the divergent diguanylate cyclase module	1156:1195	the divergent diguanylate cyclase module	1156:1195	Crystallographic and solution-based data show that BcsE's predicted GIL domain is a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*), where the divergent diguanylate cyclase module binds both dimeric c-di-GMP and BcsQ through mutually independent interfaces.
32788377	7	29	theme	activation.IMPORTANCE	1772:1792	arg1	cellulose					1804:1812	processive synthase activation.IMPORTANCE Bacterial cellulose	1752:1812	processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts	1752:1939	Together, the data suggest that BcsE acts on multiple levels to fine-tune bacterial cellulose secretion, from the early stages of secretion system assembly to the maintenance of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts.
32788377	4	30	theme	partner	993:999	arg1	BcsF					1001:1004	its intraoperon partner BcsF	977:1004	its intraoperon partner BcsF	977:1004	Here, we demonstrate that essential-for-secretion BcsR and BcsQ regulate each other's folding and stability and are recruited to the inner membrane via c-di-GMP-sensing BcsE and its intraoperon partner BcsF.
32788377	2	31	theme	biofilm	284:290	arg1	cellulose					258:266	Bacterial cellulose	248:266	Bacterial cellulose	248:266	Bacterial cellulose is a widespread biofilm component whose secretion in Gram-negative species requires an inner membrane, c-di-GMP-dependent synthase tandem (BcsAB), an outer membrane porin (BcsC), and various accessory subunits that regulate synthase assembly and function as well as the exopolysaccharide's chemical composition and mechanical properties.
32788377	2	31	theme	biofilm	284:290	arg1	component					292:300	a widespread biofilm component	271:300	a widespread biofilm component whose secretion in Gram-negative species requires an inner membrane, c-di-GMP-dependent synthase tandem (BcsAB), an outer membrane porin (BcsC), and various accessory subunits that regulate synthase assembly and function as well as the exopolysaccharide's chemical composition and mechanical properties	271:603	Bacterial cellulose is a widespread biofilm component whose secretion in Gram-negative species requires an inner membrane, c-di-GMP-dependent synthase tandem (BcsAB), an outer membrane porin (BcsC), and various accessory subunits that regulate synthase assembly and function as well as the exopolysaccharide's chemical composition and mechanical properties.
32788377	6	32	theme	BcsERQ	1401:1406	arg1	targeting					1388:1396	targeting	1388:1396	targeting of BcsERQ to the membrane	1388:1422	In addition, we reveal that a third N-terminal domain (BcsENTD) determines the protein's homooligomerization and targeting of BcsERQ to the membrane as well as previously unreported interactions with transcription antitermination complex components.
32788377	6	32	theme	BcsERQ	1401:1406	arg1	interactions					1457:1468	previously unreported interactions	1435:1468	the protein's homooligomerization and targeting of BcsERQ to the membrane as well as previously unreported interactions with transcription antitermination complex components	1350:1522	In addition, we reveal that a third N-terminal domain (BcsENTD) determines the protein's homooligomerization and targeting of BcsERQ to the membrane as well as previously unreported interactions with transcription antitermination complex components.
32788377	6	32	theme	BcsERQ	1401:1406	arg1	homooligomerization					1364:1382	the protein's homooligomerization	1350:1382	the protein's homooligomerization	1350:1382	In addition, we reveal that a third N-terminal domain (BcsENTD) determines the protein's homooligomerization and targeting of BcsERQ to the membrane as well as previously unreported interactions with transcription antitermination complex components.
32788377	5	33	theme	-GGDEF	1140:1145	arg1	domain					1117:1122	a degenerate receiver-GGDEF domain tandem	1089:1129	a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*)	1089:1147	Crystallographic and solution-based data show that BcsE's predicted GIL domain is a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*), where the divergent diguanylate cyclase module binds both dimeric c-di-GMP and BcsQ through mutually independent interfaces.
32788377	5	33	theme	-GGDEF	1140:1145	arg1	*					1146:1146	BcsEREC*-GGDEF*	1132:1146	BcsEREC*-GGDEF*	1132:1146	Crystallographic and solution-based data show that BcsE's predicted GIL domain is a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*), where the divergent diguanylate cyclase module binds both dimeric c-di-GMP and BcsQ through mutually independent interfaces.
32788377	7	34	theme	processive	1752:1761	arg1	cellulose					1804:1812	processive synthase activation.IMPORTANCE Bacterial cellulose	1752:1812	processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts	1752:1939	Together, the data suggest that BcsE acts on multiple levels to fine-tune bacterial cellulose secretion, from the early stages of secretion system assembly to the maintenance of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts.
32788377	10	35	theme	functional	2533:2542	arg1	data					2544:2547	Crystallographic and functional data	2512:2547	data	2544:2547	Crystallographic and functional data reveal that BcsE features unexpected domain architecture and likely acts on multiple levels to fine-tune bacterial cellulose production, from the early stages of secretion system assembly to the maintenence of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.
32788377	10	36	theme	assembly	2728:2735	arg1	stages					2701:2706	the early stages	2691:2706	the early stages of secretion system assembly	2691:2735	Crystallographic and functional data reveal that BcsE features unexpected domain architecture and likely acts on multiple levels to fine-tune bacterial cellulose production, from the early stages of secretion system assembly to the maintenence of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.
32788377	2	37	theme	membrane	424:431	arg1	BcsC					440:443	BcsC	440:443	BcsC	440:443	Bacterial cellulose is a widespread biofilm component whose secretion in Gram-negative species requires an inner membrane, c-di-GMP-dependent synthase tandem (BcsAB), an outer membrane porin (BcsC), and various accessory subunits that regulate synthase assembly and function as well as the exopolysaccharide's chemical composition and mechanical properties.
32788377	2	37	theme	membrane	424:431	arg1	porin					433:437	an outer membrane porin	415:437	an outer membrane porin (BcsC)	415:444	Bacterial cellulose is a widespread biofilm component whose secretion in Gram-negative species requires an inner membrane, c-di-GMP-dependent synthase tandem (BcsAB), an outer membrane porin (BcsC), and various accessory subunits that regulate synthase assembly and function as well as the exopolysaccharide's chemical composition and mechanical properties.
32788377	8	38	theme	sophisticated	2104:2116	arg1	systems					2154:2160	sophisticated Escherichia coli-like Bcs secretion systems	2104:2160	sophisticated Escherichia coli-like Bcs secretion systems	2104:2160	Whereas its secretion generally involves an inner membrane c-di-GMP-dependent synthase tandem (BcsAB) across the bacterial domain of life, enterobacteria feature sophisticated Escherichia coli-like Bcs secretion systems, where multiple additional subunits are either required for secretion or contribute to the maximal production of the polysaccharide in vivo.
32788377	1	39	theme	Most	87:90	arg1	bacteria					92:99	Most bacteria	87:99	Most bacteria	87:99	Most bacteria respond to surfaces by biogenesis of intracellular c-di-GMP, which inhibits motility and induces secretion of biofilm-promoting adherence factors.
32788377	7	40	theme	c-di-GMP	1739:1746	arg1	pool					1723:1726	a membrane-proximal pool	1703:1726	a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts	1703:1939	Together, the data suggest that BcsE acts on multiple levels to fine-tune bacterial cellulose secretion, from the early stages of secretion system assembly to the maintenance of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts.
32788377	2	41	theme	accessory	459:467	arg1	subunits					469:476	various accessory subunits	451:476	various accessory subunits	451:476	Bacterial cellulose is a widespread biofilm component whose secretion in Gram-negative species requires an inner membrane, c-di-GMP-dependent synthase tandem (BcsAB), an outer membrane porin (BcsC), and various accessory subunits that regulate synthase assembly and function as well as the exopolysaccharide's chemical composition and mechanical properties.
32788377	10	42	theme	membrane-proximal	2761:2777	arg1	pool					2779:2782	a membrane-proximal pool	2759:2782	a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation	2759:2837	Crystallographic and functional data reveal that BcsE features unexpected domain architecture and likely acts on multiple levels to fine-tune bacterial cellulose production, from the early stages of secretion system assembly to the maintenence of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.
32788377	6	43	theme	antitermination	1489:1503	arg1	components					1513:1522	transcription antitermination complex components	1475:1522	transcription antitermination complex components	1475:1522	In addition, we reveal that a third N-terminal domain (BcsENTD) determines the protein's homooligomerization and targeting of BcsERQ to the membrane as well as previously unreported interactions with transcription antitermination complex components.
32788377	5	44	theme	dimeric	1208:1214	arg1	c-di-GMP					1216:1223	dimeric c-di-GMP	1208:1223	dimeric c-di-GMP	1208:1223	Crystallographic and solution-based data show that BcsE's predicted GIL domain is a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*), where the divergent diguanylate cyclase module binds both dimeric c-di-GMP and BcsQ through mutually independent interfaces.
32788377	6	45	with	interactions	1457:1468	arg1	components					1513:1522	transcription antitermination complex components	1475:1522	transcription antitermination complex components	1475:1522	In addition, we reveal that a third N-terminal domain (BcsENTD) determines the protein's homooligomerization and targeting of BcsERQ to the membrane as well as previously unreported interactions with transcription antitermination complex components.
32788377	8	46	theme	secretion	2144:2152	arg1	systems					2154:2160	sophisticated Escherichia coli-like Bcs secretion systems	2104:2160	sophisticated Escherichia coli-like Bcs secretion systems	2104:2160	Whereas its secretion generally involves an inner membrane c-di-GMP-dependent synthase tandem (BcsAB) across the bacterial domain of life, enterobacteria feature sophisticated Escherichia coli-like Bcs secretion systems, where multiple additional subunits are either required for secretion or contribute to the maximal production of the polysaccharide in vivo.
32788377	3	47	dep	role	724:727	arg1	the					720:722	the	720:722	the	720:722	We recently showed that in Escherichia coli, most Bcs proteins form a megadalton-sized secretory nanomachine, but the role and structure of individual regulatory components remained enigmatic.
32788377	7	48	theme	fine-tune	1589:1597	arg1	secretion					1619:1627	fine-tune bacterial cellulose secretion	1589:1627	fine-tune bacterial cellulose secretion	1589:1627	Together, the data suggest that BcsE acts on multiple levels to fine-tune bacterial cellulose secretion, from the early stages of secretion system assembly to the maintenance of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts.
32788377	7	49	theme	membrane-proximal	1705:1721	arg1	pool					1723:1726	a membrane-proximal pool	1703:1726	a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts	1703:1939	Together, the data suggest that BcsE acts on multiple levels to fine-tune bacterial cellulose secretion, from the early stages of secretion system assembly to the maintenance of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts.
32788377	2	50	theme	synthase	492:499	arg1	assembly					501:508	synthase assembly	492:508	synthase assembly	492:508	Bacterial cellulose is a widespread biofilm component whose secretion in Gram-negative species requires an inner membrane, c-di-GMP-dependent synthase tandem (BcsAB), an outer membrane porin (BcsC), and various accessory subunits that regulate synthase assembly and function as well as the exopolysaccharide's chemical composition and mechanical properties.
32788377	10	51	theme	c-di-GMP	2795:2802	arg1	pool					2779:2782	a membrane-proximal pool	2759:2782	a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation	2759:2837	Crystallographic and functional data reveal that BcsE features unexpected domain architecture and likely acts on multiple levels to fine-tune bacterial cellulose production, from the early stages of secretion system assembly to the maintenence of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.
32788377	6	52	theme	unreported	1446:1455	arg1	interactions					1457:1468	previously unreported interactions	1435:1468	the protein's homooligomerization and targeting of BcsERQ to the membrane as well as previously unreported interactions with transcription antitermination complex components	1350:1522	In addition, we reveal that a third N-terminal domain (BcsENTD) determines the protein's homooligomerization and targeting of BcsERQ to the membrane as well as previously unreported interactions with transcription antitermination complex components.
32788377	5	53	theme	cyclase	1182:1188	arg1	module					1190:1195	the divergent diguanylate cyclase module	1156:1195	the divergent diguanylate cyclase module	1156:1195	Crystallographic and solution-based data show that BcsE's predicted GIL domain is a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*), where the divergent diguanylate cyclase module binds both dimeric c-di-GMP and BcsQ through mutually independent interfaces.
32788377	5	54	theme	GIL	1075:1077	arg1	domain					1117:1122	a degenerate receiver-GGDEF domain tandem	1089:1129	a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*)	1089:1147	Crystallographic and solution-based data show that BcsE's predicted GIL domain is a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*), where the divergent diguanylate cyclase module binds both dimeric c-di-GMP and BcsQ through mutually independent interfaces.
32788377	5	54	theme	GIL	1075:1077	arg1	domain					1079:1084	BcsE's predicted GIL domain	1058:1084	BcsE's predicted GIL domain	1058:1084	Crystallographic and solution-based data show that BcsE's predicted GIL domain is a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*), where the divergent diguanylate cyclase module binds both dimeric c-di-GMP and BcsQ through mutually independent interfaces.
32788377	2	55	theme	synthase	390:397	arg1	BcsAB					407:411	c-di-GMP-dependent synthase tandem (BcsAB)	371:412	c-di-GMP-dependent synthase tandem (BcsAB)	371:412	Bacterial cellulose is a widespread biofilm component whose secretion in Gram-negative species requires an inner membrane, c-di-GMP-dependent synthase tandem (BcsAB), an outer membrane porin (BcsC), and various accessory subunits that regulate synthase assembly and function as well as the exopolysaccharide's chemical composition and mechanical properties.
32788377	0	56	theme	Cellulose	51:59	arg1	BcsE					81:84	the c-di-GMP-Sensing Cellulose Secretion Regulator BcsE	30:84	the c-di-GMP-Sensing Cellulose Secretion Regulator BcsE	30:84	Structure and Multitasking of the c-di-GMP-Sensing Cellulose Secretion Regulator BcsE.
32788377	7	57	theme	infected	1926:1933	arg1	hosts					1935:1939	infected hosts	1926:1939	infected hosts	1926:1939	Together, the data suggest that BcsE acts on multiple levels to fine-tune bacterial cellulose secretion, from the early stages of secretion system assembly to the maintenance of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts.
32788377	4	58	theme	essential-for-secretion	825:847	arg1	BcsR					849:852	essential-for-secretion BcsR	825:852	essential-for-secretion BcsR	825:852	Here, we demonstrate that essential-for-secretion BcsR and BcsQ regulate each other's folding and stability and are recruited to the inner membrane via c-di-GMP-sensing BcsE and its intraoperon partner BcsF.
32788377	9	59	theme	c-di-GMP-sensing	2455:2470	arg1	BcsE					2472:2475	c-di-GMP-sensing BcsE	2455:2475	c-di-GMP-sensing BcsE	2455:2475	Here, we demonstrate that essential-for-secretion BcsR and BcsQ regulate each other's folding and stability and are recruited to the inner membrane via c-di-GMP-sensing BcsE and its intraoperon partner, BcsF.
32788377	8	60	theme	maximal	2253:2259	arg1	production					2261:2270	the maximal production	2249:2270	the maximal production of the polysaccharide	2249:2292	Whereas its secretion generally involves an inner membrane c-di-GMP-dependent synthase tandem (BcsAB) across the bacterial domain of life, enterobacteria feature sophisticated Escherichia coli-like Bcs secretion systems, where multiple additional subunits are either required for secretion or contribute to the maximal production of the polysaccharide in vivo.
32788377	3	61	theme	secretory	693:701	arg1	nanomachine					703:713	a megadalton-sized secretory nanomachine	674:713	a megadalton-sized secretory nanomachine	674:713	We recently showed that in Escherichia coli, most Bcs proteins form a megadalton-sized secretory nanomachine, but the role and structure of individual regulatory components remained enigmatic.
32788377	10	62	theme	processive	2808:2817	arg1	activation					2828:2837	processive synthase activation	2808:2837	processive synthase activation	2808:2837	Crystallographic and functional data reveal that BcsE features unexpected domain architecture and likely acts on multiple levels to fine-tune bacterial cellulose production, from the early stages of secretion system assembly to the maintenence of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.
32788377	8	63	theme	additional	2178:2187	arg1	subunits					2189:2196	multiple additional subunits	2169:2196	multiple additional subunits	2169:2196	Whereas its secretion generally involves an inner membrane c-di-GMP-dependent synthase tandem (BcsAB) across the bacterial domain of life, enterobacteria feature sophisticated Escherichia coli-like Bcs secretion systems, where multiple additional subunits are either required for secretion or contribute to the maximal production of the polysaccharide in vivo.
32788377	9	64	theme	intraoperon	2485:2495	arg1	BcsF					2506:2509	BcsF	2506:2509	BcsF	2506:2509	Here, we demonstrate that essential-for-secretion BcsR and BcsQ regulate each other's folding and stability and are recruited to the inner membrane via c-di-GMP-sensing BcsE and its intraoperon partner, BcsF.
32788377	9	64	theme	intraoperon	2485:2495	arg1	partner					2497:2503	its intraoperon partner	2481:2503	its intraoperon partner	2481:2503	Here, we demonstrate that essential-for-secretion BcsR and BcsQ regulate each other's folding and stability and are recruited to the inner membrane via c-di-GMP-sensing BcsE and its intraoperon partner, BcsF.
32788377	8	65	theme	polysaccharide	2279:2292	arg1	production					2261:2270	the maximal production	2249:2270	the maximal production of the polysaccharide	2249:2292	Whereas its secretion generally involves an inner membrane c-di-GMP-dependent synthase tandem (BcsAB) across the bacterial domain of life, enterobacteria feature sophisticated Escherichia coli-like Bcs secretion systems, where multiple additional subunits are either required for secretion or contribute to the maximal production of the polysaccharide in vivo.
32788377	6	66	theme	N-terminal	1311:1320	arg1	BcsENTD					1330:1336	BcsENTD	1330:1336	BcsENTD	1330:1336	In addition, we reveal that a third N-terminal domain (BcsENTD) determines the protein's homooligomerization and targeting of BcsERQ to the membrane as well as previously unreported interactions with transcription antitermination complex components.
32788377	6	66	theme	N-terminal	1311:1320	arg1	domain					1322:1327	a third N-terminal domain	1303:1327	a third N-terminal domain (BcsENTD)	1303:1337	In addition, we reveal that a third N-terminal domain (BcsENTD) determines the protein's homooligomerization and targeting of BcsERQ to the membrane as well as previously unreported interactions with transcription antitermination complex components.
32788377	2	67	theme	Gram-negative	321:333	arg1	species					335:341	Gram-negative species	321:341	Gram-negative species	321:341	Bacterial cellulose is a widespread biofilm component whose secretion in Gram-negative species requires an inner membrane, c-di-GMP-dependent synthase tandem (BcsAB), an outer membrane porin (BcsC), and various accessory subunits that regulate synthase assembly and function as well as the exopolysaccharide's chemical composition and mechanical properties.
32788377	5	68	theme	independent	1251:1261	arg1	interfaces					1263:1272	mutually independent interfaces	1242:1272	mutually independent interfaces	1242:1272	Crystallographic and solution-based data show that BcsE's predicted GIL domain is a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*), where the divergent diguanylate cyclase module binds both dimeric c-di-GMP and BcsQ through mutually independent interfaces.
32788377	3	69	theme	regulatory	757:766	arg1	components					768:777	individual regulatory components	746:777	individual regulatory components	746:777	We recently showed that in Escherichia coli, most Bcs proteins form a megadalton-sized secretory nanomachine, but the role and structure of individual regulatory components remained enigmatic.
32788377	8	70	theme	inner	1986:1990	arg1	BcsAB					2037:2041	an inner membrane c-di-GMP-dependent synthase tandem (BcsAB)	1983:2042	an inner membrane c-di-GMP-dependent synthase tandem (BcsAB) across the bacterial domain of life	1983:2078	Whereas its secretion generally involves an inner membrane c-di-GMP-dependent synthase tandem (BcsAB) across the bacterial domain of life, enterobacteria feature sophisticated Escherichia coli-like Bcs secretion systems, where multiple additional subunits are either required for secretion or contribute to the maximal production of the polysaccharide in vivo.
32788377	8	71	theme	multiple	2169:2176	arg1	subunits					2189:2196	multiple additional subunits	2169:2196	multiple additional subunits	2169:2196	Whereas its secretion generally involves an inner membrane c-di-GMP-dependent synthase tandem (BcsAB) across the bacterial domain of life, enterobacteria feature sophisticated Escherichia coli-like Bcs secretion systems, where multiple additional subunits are either required for secretion or contribute to the maximal production of the polysaccharide in vivo.
32788377	10	72	theme	multiple	2625:2632	arg1	levels					2634:2639	multiple levels	2625:2639	multiple levels	2625:2639	Crystallographic and functional data reveal that BcsE features unexpected domain architecture and likely acts on multiple levels to fine-tune bacterial cellulose production, from the early stages of secretion system assembly to the maintenence of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.
32788377	8	73	theme	c-di-GMP-dependent	2001:2018	arg1	BcsAB					2037:2041	an inner membrane c-di-GMP-dependent synthase tandem (BcsAB)	1983:2042	an inner membrane c-di-GMP-dependent synthase tandem (BcsAB) across the bacterial domain of life	1983:2078	Whereas its secretion generally involves an inner membrane c-di-GMP-dependent synthase tandem (BcsAB) across the bacterial domain of life, enterobacteria feature sophisticated Escherichia coli-like Bcs secretion systems, where multiple additional subunits are either required for secretion or contribute to the maximal production of the polysaccharide in vivo.
32788377	2	74	theme	inner	355:359	arg1	membrane					361:368	an inner membrane	352:368	an inner membrane	352:368	Bacterial cellulose is a widespread biofilm component whose secretion in Gram-negative species requires an inner membrane, c-di-GMP-dependent synthase tandem (BcsAB), an outer membrane porin (BcsC), and various accessory subunits that regulate synthase assembly and function as well as the exopolysaccharide's chemical composition and mechanical properties.
32788377	6	75	with	homooligomerization	1364:1382	arg1	components					1513:1522	transcription antitermination complex components	1475:1522	transcription antitermination complex components	1475:1522	In addition, we reveal that a third N-terminal domain (BcsENTD) determines the protein's homooligomerization and targeting of BcsERQ to the membrane as well as previously unreported interactions with transcription antitermination complex components.
32788377	8	76	theme	tandem	2029:2034	arg1	BcsAB					2037:2041	an inner membrane c-di-GMP-dependent synthase tandem (BcsAB)	1983:2042	an inner membrane c-di-GMP-dependent synthase tandem (BcsAB) across the bacterial domain of life	1983:2078	Whereas its secretion generally involves an inner membrane c-di-GMP-dependent synthase tandem (BcsAB) across the bacterial domain of life, enterobacteria feature sophisticated Escherichia coli-like Bcs secretion systems, where multiple additional subunits are either required for secretion or contribute to the maximal production of the polysaccharide in vivo.
32788377	4	77	theme	c-di-GMP-sensing	951:966	arg1	BcsE					968:971	c-di-GMP-sensing BcsE	951:971	c-di-GMP-sensing BcsE	951:971	Here, we demonstrate that essential-for-secretion BcsR and BcsQ regulate each other's folding and stability and are recruited to the inner membrane via c-di-GMP-sensing BcsE and its intraoperon partner BcsF.
32788377	7	78	dep	environment	1910:1920	arg1	the					1906:1908	the	1906:1908	the	1906:1908	Together, the data suggest that BcsE acts on multiple levels to fine-tune bacterial cellulose secretion, from the early stages of secretion system assembly to the maintenance of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts.
32788377	7	78	dep	environment	1910:1920	arg1	both					1898:1901	both	1898:1901	both	1898:1901	Together, the data suggest that BcsE acts on multiple levels to fine-tune bacterial cellulose secretion, from the early stages of secretion system assembly to the maintenance of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts.
32788377	10	79	theme	bacterial	2654:2662	arg1	production					2674:2683	fine-tune bacterial cellulose production	2644:2683	fine-tune bacterial cellulose production	2644:2683	Crystallographic and functional data reveal that BcsE features unexpected domain architecture and likely acts on multiple levels to fine-tune bacterial cellulose production, from the early stages of secretion system assembly to the maintenence of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.
32788377	1	80	theme	adherence	229:237	arg1	factors					239:245	biofilm-promoting adherence factors	211:245	biofilm-promoting adherence factors	211:245	Most bacteria respond to surfaces by biogenesis of intracellular c-di-GMP, which inhibits motility and induces secretion of biofilm-promoting adherence factors.
32788377	7	81	theme	assembly	1672:1679	arg1	stages					1645:1650	the early stages	1635:1650	the early stages of secretion system assembly	1635:1679	Together, the data suggest that BcsE acts on multiple levels to fine-tune bacterial cellulose secretion, from the early stages of secretion system assembly to the maintenance of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts.
32788377	5	82	theme	*	1139:1139	arg1	domain					1117:1122	a degenerate receiver-GGDEF domain tandem	1089:1129	a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*)	1089:1147	Crystallographic and solution-based data show that BcsE's predicted GIL domain is a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*), where the divergent diguanylate cyclase module binds both dimeric c-di-GMP and BcsQ through mutually independent interfaces.
32788377	5	82	theme	*	1139:1139	arg1	*					1146:1146	BcsEREC*-GGDEF*	1132:1146	BcsEREC*-GGDEF*	1132:1146	Crystallographic and solution-based data show that BcsE's predicted GIL domain is a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*), where the divergent diguanylate cyclase module binds both dimeric c-di-GMP and BcsQ through mutually independent interfaces.
32788377	2	83	theme	Bacterial	248:256	arg1	cellulose					258:266	Bacterial cellulose	248:266	Bacterial cellulose	248:266	Bacterial cellulose is a widespread biofilm component whose secretion in Gram-negative species requires an inner membrane, c-di-GMP-dependent synthase tandem (BcsAB), an outer membrane porin (BcsC), and various accessory subunits that regulate synthase assembly and function as well as the exopolysaccharide's chemical composition and mechanical properties.
32788377	2	83	theme	Bacterial	248:256	arg1	component					292:300	a widespread biofilm component	271:300	a widespread biofilm component whose secretion in Gram-negative species requires an inner membrane, c-di-GMP-dependent synthase tandem (BcsAB), an outer membrane porin (BcsC), and various accessory subunits that regulate synthase assembly and function as well as the exopolysaccharide's chemical composition and mechanical properties	271:603	Bacterial cellulose is a widespread biofilm component whose secretion in Gram-negative species requires an inner membrane, c-di-GMP-dependent synthase tandem (BcsAB), an outer membrane porin (BcsC), and various accessory subunits that regulate synthase assembly and function as well as the exopolysaccharide's chemical composition and mechanical properties.
32788377	7	84	theme	secretion	1655:1663	arg1	system					1665:1670	secretion system	1655:1670	secretion system assembly	1655:1679	Together, the data suggest that BcsE acts on multiple levels to fine-tune bacterial cellulose secretion, from the early stages of secretion system assembly to the maintenance of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts.
32788377	9	85	theme	essential-for-secretion	2329:2351	arg1	BcsR					2353:2356	essential-for-secretion BcsR	2329:2356	essential-for-secretion BcsR	2329:2356	Here, we demonstrate that essential-for-secretion BcsR and BcsQ regulate each other's folding and stability and are recruited to the inner membrane via c-di-GMP-sensing BcsE and its intraoperon partner, BcsF.
32788377	8	86	theme	bacterial	2055:2063	arg1	life					2075:2078	life	2075:2078	life	2075:2078	Whereas its secretion generally involves an inner membrane c-di-GMP-dependent synthase tandem (BcsAB) across the bacterial domain of life, enterobacteria feature sophisticated Escherichia coli-like Bcs secretion systems, where multiple additional subunits are either required for secretion or contribute to the maximal production of the polysaccharide in vivo.
32788377	8	86	theme	bacterial	2055:2063	arg1	domain					2065:2070	the bacterial domain	2051:2070	the bacterial domain of life	2051:2078	Whereas its secretion generally involves an inner membrane c-di-GMP-dependent synthase tandem (BcsAB) across the bacterial domain of life, enterobacteria feature sophisticated Escherichia coli-like Bcs secretion systems, where multiple additional subunits are either required for secretion or contribute to the maximal production of the polysaccharide in vivo.
32788377	5	87	theme	degenerate	1091:1100	arg1	domain					1117:1122	a degenerate receiver-GGDEF domain tandem	1089:1129	a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*)	1089:1147	Crystallographic and solution-based data show that BcsE's predicted GIL domain is a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*), where the divergent diguanylate cyclase module binds both dimeric c-di-GMP and BcsQ through mutually independent interfaces.
32788377	5	87	theme	degenerate	1091:1100	arg1	domain					1079:1084	BcsE's predicted GIL domain	1058:1084	BcsE's predicted GIL domain	1058:1084	Crystallographic and solution-based data show that BcsE's predicted GIL domain is a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*), where the divergent diguanylate cyclase module binds both dimeric c-di-GMP and BcsQ through mutually independent interfaces.
32788377	5	87	theme	degenerate	1091:1100	arg1	*					1146:1146	BcsEREC*-GGDEF*	1132:1146	BcsEREC*-GGDEF*	1132:1146	Crystallographic and solution-based data show that BcsE's predicted GIL domain is a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*), where the divergent diguanylate cyclase module binds both dimeric c-di-GMP and BcsQ through mutually independent interfaces.
32788377	2	88	theme	mechanical	583:592	arg1	properties					594:603	mechanical properties	583:603	mechanical properties	583:603	Bacterial cellulose is a widespread biofilm component whose secretion in Gram-negative species requires an inner membrane, c-di-GMP-dependent synthase tandem (BcsAB), an outer membrane porin (BcsC), and various accessory subunits that regulate synthase assembly and function as well as the exopolysaccharide's chemical composition and mechanical properties.
32788377	0	89	theme	BcsE	81:84	arg1	Multitasking					14:25	Multitasking	14:25	Multitasking	14:25	Structure and Multitasking of the c-di-GMP-Sensing Cellulose Secretion Regulator BcsE.
32788377	0	89	theme	BcsE	81:84	arg1	Structure					0:8	Structure	0:8	Structure	0:8	Structure and Multitasking of the c-di-GMP-Sensing Cellulose Secretion Regulator BcsE.
32788377	2	90	theme	widespread	273:282	arg1	cellulose					258:266	Bacterial cellulose	248:266	Bacterial cellulose	248:266	Bacterial cellulose is a widespread biofilm component whose secretion in Gram-negative species requires an inner membrane, c-di-GMP-dependent synthase tandem (BcsAB), an outer membrane porin (BcsC), and various accessory subunits that regulate synthase assembly and function as well as the exopolysaccharide's chemical composition and mechanical properties.
32788377	2	90	theme	widespread	273:282	arg1	component					292:300	a widespread biofilm component	271:300	a widespread biofilm component whose secretion in Gram-negative species requires an inner membrane, c-di-GMP-dependent synthase tandem (BcsAB), an outer membrane porin (BcsC), and various accessory subunits that regulate synthase assembly and function as well as the exopolysaccharide's chemical composition and mechanical properties	271:603	Bacterial cellulose is a widespread biofilm component whose secretion in Gram-negative species requires an inner membrane, c-di-GMP-dependent synthase tandem (BcsAB), an outer membrane porin (BcsC), and various accessory subunits that regulate synthase assembly and function as well as the exopolysaccharide's chemical composition and mechanical properties.
32788377	6	91	with	targeting	1388:1396	arg1	components					1513:1522	transcription antitermination complex components	1475:1522	transcription antitermination complex components	1475:1522	In addition, we reveal that a third N-terminal domain (BcsENTD) determines the protein's homooligomerization and targeting of BcsERQ to the membrane as well as previously unreported interactions with transcription antitermination complex components.
32788377	1	92	theme	c-di-GMP	152:159	arg1	biogenesis					124:133	biogenesis	124:133	biogenesis of intracellular c-di-GMP, which inhibits motility and induces secretion of biofilm-promoting adherence factors	124:245	Most bacteria respond to surfaces by biogenesis of intracellular c-di-GMP, which inhibits motility and induces secretion of biofilm-promoting adherence factors.
32788377	7	93	theme	widespread	1819:1828	arg1	component					1838:1846	a widespread biofilm component	1817:1846	a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts	1817:1939	Together, the data suggest that BcsE acts on multiple levels to fine-tune bacterial cellulose secretion, from the early stages of secretion system assembly to the maintenance of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts.
32788377	10	94	theme	early	2695:2699	arg1	stages					2701:2706	the early stages	2691:2706	the early stages of secretion system assembly	2691:2735	Crystallographic and functional data reveal that BcsE features unexpected domain architecture and likely acts on multiple levels to fine-tune bacterial cellulose production, from the early stages of secretion system assembly to the maintenence of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.
32788377	5	95	theme	diguanylate	1170:1180	arg1	module					1190:1195	the divergent diguanylate cyclase module	1156:1195	the divergent diguanylate cyclase module	1156:1195	Crystallographic and solution-based data show that BcsE's predicted GIL domain is a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*), where the divergent diguanylate cyclase module binds both dimeric c-di-GMP and BcsQ through mutually independent interfaces.
32788377	5	96	theme	Crystallographic	1007:1022	arg1	data					1043:1046	Crystallographic and solution-based data	1007:1046	data	1043:1046	Crystallographic and solution-based data show that BcsE's predicted GIL domain is a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*), where the divergent diguanylate cyclase module binds both dimeric c-di-GMP and BcsQ through mutually independent interfaces.
32788377	7	97	theme	Bacterial	1794:1802	arg1	cellulose					1804:1812	processive synthase activation.IMPORTANCE Bacterial cellulose	1752:1812	processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts	1752:1939	Together, the data suggest that BcsE acts on multiple levels to fine-tune bacterial cellulose secretion, from the early stages of secretion system assembly to the maintenance of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts.
32788377	7	98	theme	cellulose	1609:1617	arg1	secretion					1619:1627	fine-tune bacterial cellulose secretion	1589:1627	fine-tune bacterial cellulose secretion	1589:1627	Together, the data suggest that BcsE acts on multiple levels to fine-tune bacterial cellulose secretion, from the early stages of secretion system assembly to the maintenance of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts.
32788377	4	99	theme	intraoperon	981:991	arg1	BcsF					1001:1004	its intraoperon partner BcsF	977:1004	its intraoperon partner BcsF	977:1004	Here, we demonstrate that essential-for-secretion BcsR and BcsQ regulate each other's folding and stability and are recruited to the inner membrane via c-di-GMP-sensing BcsE and its intraoperon partner BcsF.
32788377	8	100	theme	Escherichia	2118:2128	arg1	systems					2154:2160	sophisticated Escherichia coli-like Bcs secretion systems	2104:2160	sophisticated Escherichia coli-like Bcs secretion systems	2104:2160	Whereas its secretion generally involves an inner membrane c-di-GMP-dependent synthase tandem (BcsAB) across the bacterial domain of life, enterobacteria feature sophisticated Escherichia coli-like Bcs secretion systems, where multiple additional subunits are either required for secretion or contribute to the maximal production of the polysaccharide in vivo.
32788377	5	101	theme	solution-based	1028:1041	arg1	data					1043:1046	Crystallographic and solution-based data	1007:1046	data	1043:1046	Crystallographic and solution-based data show that BcsE's predicted GIL domain is a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*), where the divergent diguanylate cyclase module binds both dimeric c-di-GMP and BcsQ through mutually independent interfaces.
32788377	7	102	theme	synthase	1763:1770	arg1	cellulose					1804:1812	processive synthase activation.IMPORTANCE Bacterial cellulose	1752:1812	processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts	1752:1939	Together, the data suggest that BcsE acts on multiple levels to fine-tune bacterial cellulose secretion, from the early stages of secretion system assembly to the maintenance of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts.
32788377	10	103	theme	system	2721:2726	arg1	assembly					2728:2735	secretion system assembly	2711:2735	secretion system assembly	2711:2735	Crystallographic and functional data reveal that BcsE features unexpected domain architecture and likely acts on multiple levels to fine-tune bacterial cellulose production, from the early stages of secretion system assembly to the maintenence of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.
32788377	2	104	theme	outer	418:422	arg1	BcsC					440:443	BcsC	440:443	BcsC	440:443	Bacterial cellulose is a widespread biofilm component whose secretion in Gram-negative species requires an inner membrane, c-di-GMP-dependent synthase tandem (BcsAB), an outer membrane porin (BcsC), and various accessory subunits that regulate synthase assembly and function as well as the exopolysaccharide's chemical composition and mechanical properties.
32788377	2	104	theme	outer	418:422	arg1	porin					433:437	an outer membrane porin	415:437	an outer membrane porin (BcsC)	415:444	Bacterial cellulose is a widespread biofilm component whose secretion in Gram-negative species requires an inner membrane, c-di-GMP-dependent synthase tandem (BcsAB), an outer membrane porin (BcsC), and various accessory subunits that regulate synthase assembly and function as well as the exopolysaccharide's chemical composition and mechanical properties.
32788377	6	105	theme	complex	1505:1511	arg1	components					1513:1522	transcription antitermination complex components	1475:1522	transcription antitermination complex components	1475:1522	In addition, we reveal that a third N-terminal domain (BcsENTD) determines the protein's homooligomerization and targeting of BcsERQ to the membrane as well as previously unreported interactions with transcription antitermination complex components.
32788377	2	106	theme	various	451:457	arg1	subunits					469:476	various accessory subunits	451:476	various accessory subunits	451:476	Bacterial cellulose is a widespread biofilm component whose secretion in Gram-negative species requires an inner membrane, c-di-GMP-dependent synthase tandem (BcsAB), an outer membrane porin (BcsC), and various accessory subunits that regulate synthase assembly and function as well as the exopolysaccharide's chemical composition and mechanical properties.
32788377	7	107	dep	cellulose	1804:1812	arg1	component					1838:1846	a widespread biofilm component	1817:1846	a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts	1817:1939	Together, the data suggest that BcsE acts on multiple levels to fine-tune bacterial cellulose secretion, from the early stages of secretion system assembly to the maintenance of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts.
32788377	6	108	theme	transcription	1475:1487	arg1	components					1513:1522	transcription antitermination complex components	1475:1522	transcription antitermination complex components	1475:1522	In addition, we reveal that a third N-terminal domain (BcsENTD) determines the protein's homooligomerization and targeting of BcsERQ to the membrane as well as previously unreported interactions with transcription antitermination complex components.
32788377	7	109	theme	dimeric	1731:1737	arg1	c-di-GMP					1739:1746	dimeric c-di-GMP	1731:1746	dimeric c-di-GMP for processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts	1731:1939	Together, the data suggest that BcsE acts on multiple levels to fine-tune bacterial cellulose secretion, from the early stages of secretion system assembly to the maintenance of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts.
32788377	0	110	theme	c-di-GMP-Sensing	34:49	arg1	BcsE					81:84	the c-di-GMP-Sensing Cellulose Secretion Regulator BcsE	30:84	the c-di-GMP-Sensing Cellulose Secretion Regulator BcsE	30:84	Structure and Multitasking of the c-di-GMP-Sensing Cellulose Secretion Regulator BcsE.
32788377	7	111	theme	bacterial	1599:1607	arg1	secretion					1619:1627	fine-tune bacterial cellulose secretion	1589:1627	fine-tune bacterial cellulose secretion	1589:1627	Together, the data suggest that BcsE acts on multiple levels to fine-tune bacterial cellulose secretion, from the early stages of secretion system assembly to the maintenance of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts.
32788377	5	112	theme	predicted	1065:1073	arg1	domain					1117:1122	a degenerate receiver-GGDEF domain tandem	1089:1129	a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*)	1089:1147	Crystallographic and solution-based data show that BcsE's predicted GIL domain is a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*), where the divergent diguanylate cyclase module binds both dimeric c-di-GMP and BcsQ through mutually independent interfaces.
32788377	5	112	theme	predicted	1065:1073	arg1	domain					1079:1084	BcsE's predicted GIL domain	1058:1084	BcsE's predicted GIL domain	1058:1084	Crystallographic and solution-based data show that BcsE's predicted GIL domain is a degenerate receiver-GGDEF domain tandem (BcsEREC*-GGDEF*), where the divergent diguanylate cyclase module binds both dimeric c-di-GMP and BcsQ through mutually independent interfaces.
32788377	7	113	theme	pool	1723:1726	arg1	maintenance					1688:1698	the maintenance	1684:1698	the maintenance of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts	1684:1939	Together, the data suggest that BcsE acts on multiple levels to fine-tune bacterial cellulose secretion, from the early stages of secretion system assembly to the maintenance of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts.
32788377	3	114	theme	Bcs	656:658	arg1	proteins					660:667	most Bcs proteins	651:667	most Bcs proteins	651:667	We recently showed that in Escherichia coli, most Bcs proteins form a megadalton-sized secretory nanomachine, but the role and structure of individual regulatory components remained enigmatic.
32788377	10	115	theme	dimeric	2787:2793	arg1	c-di-GMP					2795:2802	dimeric c-di-GMP	2787:2802	dimeric c-di-GMP for processive synthase activation	2787:2837	Crystallographic and functional data reveal that BcsE features unexpected domain architecture and likely acts on multiple levels to fine-tune bacterial cellulose production, from the early stages of secretion system assembly to the maintenence of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.
32788377	9	116	theme	inner	2436:2440	arg1	membrane					2442:2449	the inner membrane	2432:2449	the inner membrane	2432:2449	Here, we demonstrate that essential-for-secretion BcsR and BcsQ regulate each other's folding and stability and are recruited to the inner membrane via c-di-GMP-sensing BcsE and its intraoperon partner, BcsF.
32788377	0	117	theme	Secretion	61:69	arg1	BcsE					81:84	the c-di-GMP-Sensing Cellulose Secretion Regulator BcsE	30:84	the c-di-GMP-Sensing Cellulose Secretion Regulator BcsE	30:84	Structure and Multitasking of the c-di-GMP-Sensing Cellulose Secretion Regulator BcsE.
32788377	10	118	theme	domain	2586:2591	arg1	architecture					2593:2604	unexpected domain architecture	2575:2604	unexpected domain architecture	2575:2604	Crystallographic and functional data reveal that BcsE features unexpected domain architecture and likely acts on multiple levels to fine-tune bacterial cellulose production, from the early stages of secretion system assembly to the maintenence of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.
32788377	10	119	theme	pool	2779:2782	arg1	maintenence					2744:2754	the maintenence	2740:2754	the maintenence of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation	2740:2837	Crystallographic and functional data reveal that BcsE features unexpected domain architecture and likely acts on multiple levels to fine-tune bacterial cellulose production, from the early stages of secretion system assembly to the maintenence of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.
32788377	2	120	theme	c-di-GMP-dependent	371:388	arg1	BcsAB					407:411	c-di-GMP-dependent synthase tandem (BcsAB)	371:412	c-di-GMP-dependent synthase tandem (BcsAB)	371:412	Bacterial cellulose is a widespread biofilm component whose secretion in Gram-negative species requires an inner membrane, c-di-GMP-dependent synthase tandem (BcsAB), an outer membrane porin (BcsC), and various accessory subunits that regulate synthase assembly and function as well as the exopolysaccharide's chemical composition and mechanical properties.
32788377	7	121	theme	multiple	1570:1577	arg1	levels					1579:1584	multiple levels	1570:1584	multiple levels	1570:1584	Together, the data suggest that BcsE acts on multiple levels to fine-tune bacterial cellulose secretion, from the early stages of secretion system assembly to the maintenance of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts.
32788377	3	122	theme	megadalton-sized	676:691	arg1	nanomachine					703:713	a megadalton-sized secretory nanomachine	674:713	a megadalton-sized secretory nanomachine	674:713	We recently showed that in Escherichia coli, most Bcs proteins form a megadalton-sized secretory nanomachine, but the role and structure of individual regulatory components remained enigmatic.
32788377	10	123	theme	synthase	2819:2826	arg1	activation					2828:2837	processive synthase activation	2808:2837	processive synthase activation	2808:2837	Crystallographic and functional data reveal that BcsE features unexpected domain architecture and likely acts on multiple levels to fine-tune bacterial cellulose production, from the early stages of secretion system assembly to the maintenence of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.
32788377	8	124	theme	Bcs	2140:2142	arg1	systems					2154:2160	sophisticated Escherichia coli-like Bcs secretion systems	2104:2160	sophisticated Escherichia coli-like Bcs secretion systems	2104:2160	Whereas its secretion generally involves an inner membrane c-di-GMP-dependent synthase tandem (BcsAB) across the bacterial domain of life, enterobacteria feature sophisticated Escherichia coli-like Bcs secretion systems, where multiple additional subunits are either required for secretion or contribute to the maximal production of the polysaccharide in vivo.
33835911	14	0	theme	genera	2289:2294	arg1	Haloactinobacterium					2296:2314	the genera Haloactinobacterium	2285:2314	the genera Haloactinobacterium	2285:2314	Based on revised chemotaxonomic and additional genome based data, it is necessary to discuss and evaluate the results in the light of the classification and nomenclature of members of the family Ruaniaceae, i.e. the genera Haloactinobacterium and Ruania.
33835911	17	1	theme	genus	2527:2531	arg1	Ruania					2533:2538	the genus Ruania	2523:2538	the genus Ruania	2523:2538	nov., with an emended description of the genus Ruania are proposed.
33835911	4	2	theme	non-motile	534:543	arg1	actinobacterium					562:576	an aerobic, non-motile and halotolerant actinobacterium	522:576	an aerobic, non-motile and halotolerant actinobacterium forming small rods or cocci	522:604	In the course of screening the surface soils of ancient copper mines and smelters (East Harz, Germany) an aerobic, non-motile and halotolerant actinobacterium forming small rods or cocci was isolated.
33835911	10	3	theme	acid	1283:1286	arg1	profile					1288:1294	The fatty acid profile	1273:1294	The fatty acid profile	1273:1294	The fatty acid profile contained mainly iso and anteiso branched fatty acids (anteiso-C15 : 0, iso-C14 : 0) and aldehydes/dimethylacetals (i.e. not fatty acids).
33835911	10	4	contain	contained	1296:1304	arg2	aldehydes/dimethylacetals					1385:1409	aldehydes/dimethylacetals	1385:1409	aldehydes/dimethylacetals (i.e. not fatty acids)	1385:1432	The fatty acid profile contained mainly iso and anteiso branched fatty acids (anteiso-C15 : 0, iso-C14 : 0) and aldehydes/dimethylacetals (i.e. not fatty acids).
33835911	10	4	contain	contained	1296:1304	arg2	anteiso-C15 					1351:1362	anteiso-C15 	1351:1362	anteiso-C15 	1351:1362	The fatty acid profile contained mainly iso and anteiso branched fatty acids (anteiso-C15 : 0, iso-C14 : 0) and aldehydes/dimethylacetals (i.e. not fatty acids).
33835911	10	4	contain	contained	1296:1304	arg2	acids					1344:1348	iso and anteiso branched fatty acids	1313:1348	iso and anteiso branched fatty acids (anteiso-C15 : 0, iso-C14 : 0)	1313:1379	The fatty acid profile contained mainly iso and anteiso branched fatty acids (anteiso-C15 : 0, iso-C14 : 0) and aldehydes/dimethylacetals (i.e. not fatty acids).
33835911	10	4	contain	contained	1296:1304	arg1	profile					1288:1294	The fatty acid profile	1273:1294	The fatty acid profile	1273:1294	The fatty acid profile contained mainly iso and anteiso branched fatty acids (anteiso-C15 : 0, iso-C14 : 0) and aldehydes/dimethylacetals (i.e. not fatty acids).
33835911	10	4	contain	contained	1296:1304	arg2	acids					1427:1431	not fatty acids	1417:1431	not fatty acids	1417:1431	The fatty acid profile contained mainly iso and anteiso branched fatty acids (anteiso-C15 : 0, iso-C14 : 0) and aldehydes/dimethylacetals (i.e. not fatty acids).
33835911	12	5	theme	Genotypic	1854:1862	arg1	analyses					1883:1890	Genotypic and chemotaxonomic analyses	1854:1890	Genotypic and chemotaxonomic analyses	1854:1890	Genotypic and chemotaxonomic analyses support the view that strain F300T (=DSM 108350T=CIP 111667T) is the type strain of a new genus and new species for which the name Occultella aeris gen. nov., sp.
33835911	4	6	theme	halotolerant	549:560	arg1	actinobacterium					562:576	an aerobic, non-motile and halotolerant actinobacterium	522:576	an aerobic, non-motile and halotolerant actinobacterium forming small rods or cocci	522:604	In the course of screening the surface soils of ancient copper mines and smelters (East Harz, Germany) an aerobic, non-motile and halotolerant actinobacterium forming small rods or cocci was isolated.
33835911	5	7	theme	designated	631:640	arg1	F300T					642:646	The strain designated F300T	620:646	The strain designated F300T	620:646	The strain designated F300T developed creamy to yellow colonies on tryptone soy agar and grew optimally at 28 °C, pH 7-8 and with 0.5-2 % (m/v) NaCl.
33835911	2	8	theme	heterotypic	273:283	arg1	names					232:236	the genus names	222:236	the genus names Haloactinobacterium and Ruania	222:267	nov, with an emended description of the genus Ruania, recognition that the genus names Haloactinobacterium and Ruania are heterotypic synonyms and description of Occultella aeris sp.
33835911	2	8	theme	heterotypic	273:283	arg1	synonyms					285:292	heterotypic synonyms	273:292	heterotypic synonyms	273:292	nov, with an emended description of the genus Ruania, recognition that the genus names Haloactinobacterium and Ruania are heterotypic synonyms and description of Occultella aeris sp.
33835911	14	9	theme	members	2246:2252	arg1	classification					2211:2224	classification	2211:2224	classification	2211:2224	Based on revised chemotaxonomic and additional genome based data, it is necessary to discuss and evaluate the results in the light of the classification and nomenclature of members of the family Ruaniaceae, i.e. the genera Haloactinobacterium and Ruania.
33835911	14	9	theme	members	2246:2252	arg1	nomenclature					2230:2241	nomenclature	2230:2241	nomenclature	2230:2241	Based on revised chemotaxonomic and additional genome based data, it is necessary to discuss and evaluate the results in the light of the classification and nomenclature of members of the family Ruaniaceae, i.e. the genera Haloactinobacterium and Ruania.
33835911	5	10	from	colonies	675:682	arg1	agar					700:703	tryptone soy agar	687:703	tryptone soy agar	687:703	The strain designated F300T developed creamy to yellow colonies on tryptone soy agar and grew optimally at 28 °C, pH 7-8 and with 0.5-2 % (m/v) NaCl.
33835911	12	11	theme	chemotaxonomic	1868:1881	arg1	analyses					1883:1890	Genotypic and chemotaxonomic analyses	1854:1890	Genotypic and chemotaxonomic analyses	1854:1890	Genotypic and chemotaxonomic analyses support the view that strain F300T (=DSM 108350T=CIP 111667T) is the type strain of a new genus and new species for which the name Occultella aeris gen. nov., sp.
33835911	14	12	theme	Ruaniaceae	2268:2277	arg1	members					2246:2252	members	2246:2252	members	2246:2252	Based on revised chemotaxonomic and additional genome based data, it is necessary to discuss and evaluate the results in the light of the classification and nomenclature of members of the family Ruaniaceae, i.e. the genera Haloactinobacterium and Ruania.
33835911	2	13	dep	names	232:236	arg1	names					232:236	the genus names	222:236	the genus names Haloactinobacterium and Ruania	222:267	nov, with an emended description of the genus Ruania, recognition that the genus names Haloactinobacterium and Ruania are heterotypic synonyms and description of Occultella aeris sp.
33835911	2	13	dep	names	232:236	arg1	Ruania					262:267	Ruania	262:267	Ruania	262:267	nov, with an emended description of the genus Ruania, recognition that the genus names Haloactinobacterium and Ruania are heterotypic synonyms and description of Occultella aeris sp.
33835911	2	13	dep	names	232:236	arg1	Haloactinobacterium					238:256	Haloactinobacterium	238:256	Haloactinobacterium	238:256	nov, with an emended description of the genus Ruania, recognition that the genus names Haloactinobacterium and Ruania are heterotypic synonyms and description of Occultella aeris sp.
33835911	10	14	theme	iso	1313:1315	arg1	anteiso-C15 					1351:1362	anteiso-C15 	1351:1362	anteiso-C15 	1351:1362	The fatty acid profile contained mainly iso and anteiso branched fatty acids (anteiso-C15 : 0, iso-C14 : 0) and aldehydes/dimethylacetals (i.e. not fatty acids).
33835911	10	14	theme	iso	1313:1315	arg1	acids					1344:1348	iso and anteiso branched fatty acids	1313:1348	iso and anteiso branched fatty acids (anteiso-C15 : 0, iso-C14 : 0)	1313:1379	The fatty acid profile contained mainly iso and anteiso branched fatty acids (anteiso-C15 : 0, iso-C14 : 0) and aldehydes/dimethylacetals (i.e. not fatty acids).
33835911	6	15	theme	A4α	800:802	arg1	A11.54					817:822	A11.54	817:822	A11.54	817:822	Its peptidoglycan was of type A4α l-Lys-l-Glu (A11.54).
33835911	6	15	theme	A4α	800:802	arg1	l-Lys-l-Glu					804:814	type A4α l-Lys-l-Glu	795:814	type A4α l-Lys-l-Glu (A11.54)	795:823	Its peptidoglycan was of type A4α l-Lys-l-Glu (A11.54).
33835911	12	16	theme	genus	1982:1986	arg1	F300T					1921:1925	strain F300T	1914:1925	strain F300T (=DSM 108350T=CIP 111667T)	1914:1952	Genotypic and chemotaxonomic analyses support the view that strain F300T (=DSM 108350T=CIP 111667T) is the type strain of a new genus and new species for which the name Occultella aeris gen. nov., sp.
33835911	12	16	theme	genus	1982:1986	arg1	strain					1966:1971	the type strain	1957:1971	the type strain of a new genus and new species for which the name Occultella aeris gen. nov., sp	1957:2052	Genotypic and chemotaxonomic analyses support the view that strain F300T (=DSM 108350T=CIP 111667T) is the type strain of a new genus and new species for which the name Occultella aeris gen. nov., sp.
33835911	7	17	theme	MK-9	938:941	arg1	H2					920:921	H2	920:921	H2	920:921	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	7	17	theme	MK-9	938:941	arg1	MK-9					938:941	MK-9	938:941	MK-9(H4)	938:945	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	7	17	theme	MK-9	938:941	arg1	amounts					904:910	minor amounts	898:910	minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4)	898:945	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	7	17	theme	MK-9	938:941	arg1	H4					943:944	H4	943:944	H4	943:944	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	7	17	theme	MK-9	938:941	arg1	MK-8					925:928	MK-8	925:928	MK-8(H6)	925:932	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	7	17	theme	MK-9	938:941	arg1	MK-8					915:918	MK-8	915:918	MK-8(H2)	915:922	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	7	17	theme	MK-9	938:941	arg1	H6					930:931	H6	930:931	H6	930:931	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	8	18	theme	phosphatidylinositol	1063:1082	arg1	phospholipids					1162:1174	two phospholipids	1158:1174	two phospholipids	1158:1174	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, mono and diacylated phosphatidylinositol dimannosides, and components that were not fully characterized, including two phospholipids, two glycolipids and an uncharacterized lipid.
33835911	8	18	theme	phosphatidylinositol	1063:1082	arg1	glycolipids					1181:1191	two glycolipids	1177:1191	two glycolipids	1177:1191	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, mono and diacylated phosphatidylinositol dimannosides, and components that were not fully characterized, including two phospholipids, two glycolipids and an uncharacterized lipid.
33835911	8	18	theme	phosphatidylinositol	1063:1082	arg1	dimannosides					1084:1095	phosphatidylinositol, mono and diacylated phosphatidylinositol dimannosides	1021:1095	dimannosides	1084:1095	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, mono and diacylated phosphatidylinositol dimannosides, and components that were not fully characterized, including two phospholipids, two glycolipids and an uncharacterized lipid.
33835911	8	18	theme	phosphatidylinositol	1063:1082	arg1	lipid					1216:1220	an uncharacterized lipid	1197:1220	an uncharacterized lipid	1197:1220	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, mono and diacylated phosphatidylinositol dimannosides, and components that were not fully characterized, including two phospholipids, two glycolipids and an uncharacterized lipid.
33835911	10	19	dep	acids	1427:1431	arg1	i.e.					1412:1415	i.e.	1412:1415	i.e.	1412:1415	The fatty acid profile contained mainly iso and anteiso branched fatty acids (anteiso-C15 : 0, iso-C14 : 0) and aldehydes/dimethylacetals (i.e. not fatty acids).
33835911	11	20	dep	Ruania	1583:1588	arg1	Ruaniaceae					1622:1631	family Ruaniaceae	1615:1631	family Ruaniaceae	1615:1631	Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales) as a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively.
33835911	11	20	dep	Ruania	1583:1588	arg1	Micrococcales					1640:1652	order Micrococcales	1634:1652	order Micrococcales	1634:1652	Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales) as a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively.
33835911	11	20	dep	Ruania	1583:1588	arg1	the					1572:1574	the	1572:1574	the	1572:1574	Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales) as a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively.
33835911	12	21	dep	name	2018:2021	arg1	nov.					2045:2048	Occultella aeris gen. nov.	2023:2048	the name Occultella aeris gen. nov.	2014:2048	Genotypic and chemotaxonomic analyses support the view that strain F300T (=DSM 108350T=CIP 111667T) is the type strain of a new genus and new species for which the name Occultella aeris gen. nov., sp.
33835911	5	22	theme	tryptone	687:694	arg1	agar					700:703	tryptone soy agar	687:703	tryptone soy agar	687:703	The strain designated F300T developed creamy to yellow colonies on tryptone soy agar and grew optimally at 28 °C, pH 7-8 and with 0.5-2 % (m/v) NaCl.
33835911	16	23	theme	Ruania	2468:2473	arg1	comb					2480:2483	Ruania alba comb	2468:2483	Ruania alba comb	2468:2483	nov. and Haloactinobacterium album as Ruania alba comb.
33835911	17	24	theme	emended	2500:2506	arg1	description					2508:2518	an emended description	2497:2518	an emended description of the genus Ruania	2497:2538	nov., with an emended description of the genus Ruania are proposed.
33835911	12	25	theme	108350T=CIP	1933:1943	arg1	F300T					1921:1925	strain F300T	1914:1925	strain F300T (=DSM 108350T=CIP 111667T)	1914:1952	Genotypic and chemotaxonomic analyses support the view that strain F300T (=DSM 108350T=CIP 111667T) is the type strain of a new genus and new species for which the name Occultella aeris gen. nov., sp.
33835911	12	25	theme	108350T=CIP	1933:1943	arg1	111667T					1945:1951	=DSM 108350T=CIP 111667T	1928:1951	=DSM 108350T=CIP 111667T	1928:1951	Genotypic and chemotaxonomic analyses support the view that strain F300T (=DSM 108350T=CIP 111667T) is the type strain of a new genus and new species for which the name Occultella aeris gen. nov., sp.
33835911	11	26	theme	sister	1660:1665	arg1	taxon					1667:1671	a sister taxon	1658:1671	a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively	1658:1851	Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales) as a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively.
33835911	7	27	theme	minor	898:902	arg1	H2					920:921	H2	920:921	H2	920:921	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	7	27	theme	minor	898:902	arg1	MK-9					938:941	MK-9	938:941	MK-9(H4)	938:945	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	7	27	theme	minor	898:902	arg1	amounts					904:910	minor amounts	898:910	minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4)	898:945	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	7	27	theme	minor	898:902	arg1	H4					943:944	H4	943:944	H4	943:944	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	7	27	theme	minor	898:902	arg1	MK-8					925:928	MK-8	925:928	MK-8(H6)	925:932	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	7	27	theme	minor	898:902	arg1	MK-8					915:918	MK-8	915:918	MK-8(H2)	915:922	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	7	27	theme	minor	898:902	arg1	H6					930:931	H6	930:931	H6	930:931	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	14	28	dep	classification	2211:2224	arg1	the					2194:2196	the	2194:2196	the	2194:2196	Based on revised chemotaxonomic and additional genome based data, it is necessary to discuss and evaluate the results in the light of the classification and nomenclature of members of the family Ruaniaceae, i.e. the genera Haloactinobacterium and Ruania.
33835911	14	28	dep	classification	2211:2224	arg1	the					2207:2209	the	2207:2209	the	2207:2209	Based on revised chemotaxonomic and additional genome based data, it is necessary to discuss and evaluate the results in the light of the classification and nomenclature of members of the family Ruaniaceae, i.e. the genera Haloactinobacterium and Ruania.
33835911	14	28	dep	classification	2211:2224	arg1	light					2198:2202	light	2198:2202	light	2198:2202	Based on revised chemotaxonomic and additional genome based data, it is necessary to discuss and evaluate the results in the light of the classification and nomenclature of members of the family Ruaniaceae, i.e. the genera Haloactinobacterium and Ruania.
33835911	11	29	theme	Haloactinobacterium	1594:1612	arg1	members					1561:1567	members	1561:1567	members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales)	1561:1653	Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales) as a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively.
33835911	1	30	theme	alba	140:143	arg1	comb					145:148	Ruania alba comb	133:148	Ruania alba comb	133:148	nov., of Haloactinobacterium album as Ruania alba comb.
33835911	12	31	theme	strain	1914:1919	arg1	F300T					1921:1925	strain F300T	1914:1925	strain F300T (=DSM 108350T=CIP 111667T)	1914:1952	Genotypic and chemotaxonomic analyses support the view that strain F300T (=DSM 108350T=CIP 111667T) is the type strain of a new genus and new species for which the name Occultella aeris gen. nov., sp.
33835911	12	31	theme	strain	1914:1919	arg1	strain					1966:1971	the type strain	1957:1971	the type strain of a new genus and new species for which the name Occultella aeris gen. nov., sp	1957:2052	Genotypic and chemotaxonomic analyses support the view that strain F300T (=DSM 108350T=CIP 111667T) is the type strain of a new genus and new species for which the name Occultella aeris gen. nov., sp.
33835911	12	31	theme	strain	1914:1919	arg1	111667T					1945:1951	=DSM 108350T=CIP 111667T	1928:1951	=DSM 108350T=CIP 111667T	1928:1951	Genotypic and chemotaxonomic analyses support the view that strain F300T (=DSM 108350T=CIP 111667T) is the type strain of a new genus and new species for which the name Occultella aeris gen. nov., sp.
33835911	11	32	theme	Ruania	1583:1588	arg1	members					1561:1567	members	1561:1567	members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales)	1561:1653	Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales) as a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively.
33835911	10	33	dep	anteiso-C15 	1351:1362	arg1	iso-C14 					1368:1375	iso-C14 	1368:1375	iso-C14 	1368:1375	The fatty acid profile contained mainly iso and anteiso branched fatty acids (anteiso-C15 : 0, iso-C14 : 0) and aldehydes/dimethylacetals (i.e. not fatty acids).
33835911	10	33	dep	anteiso-C15 	1351:1362	arg1	 0					1364:1365	 0	1364:1365	 0	1364:1365	The fatty acid profile contained mainly iso and anteiso branched fatty acids (anteiso-C15 : 0, iso-C14 : 0) and aldehydes/dimethylacetals (i.e. not fatty acids).
33835911	10	33	dep	anteiso-C15 	1351:1362	arg1	 0					1377:1378	 0	1377:1378	 0	1377:1378	The fatty acid profile contained mainly iso and anteiso branched fatty acids (anteiso-C15 : 0, iso-C14 : 0) and aldehydes/dimethylacetals (i.e. not fatty acids).
33835911	4	34	theme	copper	475:480	arg1	mines					482:486	ancient copper mines	467:486	ancient copper mines	467:486	In the course of screening the surface soils of ancient copper mines and smelters (East Harz, Germany) an aerobic, non-motile and halotolerant actinobacterium forming small rods or cocci was isolated.
33835911	10	35	theme	fatty	1338:1342	arg1	anteiso-C15 					1351:1362	anteiso-C15 	1351:1362	anteiso-C15 	1351:1362	The fatty acid profile contained mainly iso and anteiso branched fatty acids (anteiso-C15 : 0, iso-C14 : 0) and aldehydes/dimethylacetals (i.e. not fatty acids).
33835911	10	35	theme	fatty	1338:1342	arg1	acids					1344:1348	iso and anteiso branched fatty acids	1313:1348	iso and anteiso branched fatty acids (anteiso-C15 : 0, iso-C14 : 0)	1313:1379	The fatty acid profile contained mainly iso and anteiso branched fatty acids (anteiso-C15 : 0, iso-C14 : 0) and aldehydes/dimethylacetals (i.e. not fatty acids).
33835911	1	36	dep	nov.	95:98	arg1	of					101:102	of	101:102	of	101:102	nov., of Haloactinobacterium album as Ruania alba comb.
33835911	12	37	dep	sp	2051:2052	arg1	name					2018:2021	the name	2014:2021	the name Occultella aeris gen. nov.	2014:2048	Genotypic and chemotaxonomic analyses support the view that strain F300T (=DSM 108350T=CIP 111667T) is the type strain of a new genus and new species for which the name Occultella aeris gen. nov., sp.
33835911	3	38	theme	halotolerant	342:353	arg1	nov.					334:337	nov.	334:337	nov.	334:337	nov., a halotolerant isolate from surface soil sampled at an ancient copper smelter.
33835911	3	38	theme	halotolerant	342:353	arg1	isolate					355:361	a halotolerant isolate	340:361	a halotolerant isolate from surface soil	340:379	nov., a halotolerant isolate from surface soil sampled at an ancient copper smelter.
33835911	10	39	theme	anteiso	1321:1327	arg1	anteiso-C15 					1351:1362	anteiso-C15 	1351:1362	anteiso-C15 	1351:1362	The fatty acid profile contained mainly iso and anteiso branched fatty acids (anteiso-C15 : 0, iso-C14 : 0) and aldehydes/dimethylacetals (i.e. not fatty acids).
33835911	10	39	theme	anteiso	1321:1327	arg1	acids					1344:1348	iso and anteiso branched fatty acids	1313:1348	iso and anteiso branched fatty acids (anteiso-C15 : 0, iso-C14 : 0)	1313:1379	The fatty acid profile contained mainly iso and anteiso branched fatty acids (anteiso-C15 : 0, iso-C14 : 0) and aldehydes/dimethylacetals (i.e. not fatty acids).
33835911	14	40	theme	genome	2120:2125	arg1	data					2133:2136	revised chemotaxonomic and additional genome based data	2082:2136	revised chemotaxonomic and additional genome based data	2082:2136	Based on revised chemotaxonomic and additional genome based data, it is necessary to discuss and evaluate the results in the light of the classification and nomenclature of members of the family Ruaniaceae, i.e. the genera Haloactinobacterium and Ruania.
33835911	11	41	theme	family	1615:1620	arg1	Ruaniaceae					1622:1631	family Ruaniaceae	1615:1631	family Ruaniaceae	1615:1631	Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales) as a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively.
33835911	11	41	theme	family	1615:1620	arg1	Micrococcales					1640:1652	order Micrococcales	1634:1652	order Micrococcales	1634:1652	Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales) as a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively.
33835911	4	42	theme	surface	450:456	arg1	soils					458:462	the surface soils	446:462	the surface soils of ancient copper mines and smelters (East Harz, Germany)	446:520	In the course of screening the surface soils of ancient copper mines and smelters (East Harz, Germany) an aerobic, non-motile and halotolerant actinobacterium forming small rods or cocci was isolated.
33835911	3	43	from	soil	376:379	arg1	nov.					334:337	nov.	334:337	nov.	334:337	nov., a halotolerant isolate from surface soil sampled at an ancient copper smelter.
33835911	3	43	from	soil	376:379	arg1	isolate					355:361	a halotolerant isolate	340:361	a halotolerant isolate from surface soil	340:379	nov., a halotolerant isolate from surface soil sampled at an ancient copper smelter.
33835911	12	44	theme	gen.	2040:2043	arg1	nov.					2045:2048	Occultella aeris gen. nov.	2023:2048	the name Occultella aeris gen. nov.	2014:2048	Genotypic and chemotaxonomic analyses support the view that strain F300T (=DSM 108350T=CIP 111667T) is the type strain of a new genus and new species for which the name Occultella aeris gen. nov., sp.
33835911	14	45	theme	revised	2082:2088	arg1	data					2133:2136	revised chemotaxonomic and additional genome based data	2082:2136	revised chemotaxonomic and additional genome based data	2082:2136	Based on revised chemotaxonomic and additional genome based data, it is necessary to discuss and evaluate the results in the light of the classification and nomenclature of members of the family Ruaniaceae, i.e. the genera Haloactinobacterium and Ruania.
33835911	11	46	theme	average	1753:1759	arg1	identity					1772:1779	an average nucleotide identity	1750:1779	an average nucleotide identity	1750:1779	Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales) as a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively.
33835911	5	47	theme	%	756:756	arg1	NaCl					764:767	0.5-2 % (m/v) NaCl	750:767	0.5-2 % (m/v) NaCl	750:767	The strain designated F300T developed creamy to yellow colonies on tryptone soy agar and grew optimally at 28 °C, pH 7-8 and with 0.5-2 % (m/v) NaCl.
33835911	2	48	theme	genus	226:230	arg1	names					232:236	the genus names	222:236	the genus names Haloactinobacterium and Ruania	222:267	nov, with an emended description of the genus Ruania, recognition that the genus names Haloactinobacterium and Ruania are heterotypic synonyms and description of Occultella aeris sp.
33835911	2	48	theme	genus	226:230	arg1	Ruania					262:267	Ruania	262:267	Ruania	262:267	nov, with an emended description of the genus Ruania, recognition that the genus names Haloactinobacterium and Ruania are heterotypic synonyms and description of Occultella aeris sp.
33835911	2	48	theme	genus	226:230	arg1	synonyms					285:292	heterotypic synonyms	273:292	heterotypic synonyms	273:292	nov, with an emended description of the genus Ruania, recognition that the genus names Haloactinobacterium and Ruania are heterotypic synonyms and description of Occultella aeris sp.
33835911	2	48	theme	genus	226:230	arg1	Haloactinobacterium					238:256	Haloactinobacterium	238:256	Haloactinobacterium	238:256	nov, with an emended description of the genus Ruania, recognition that the genus names Haloactinobacterium and Ruania are heterotypic synonyms and description of Occultella aeris sp.
33835911	17	49	with	nov.	2486:2489	arg1	description					2508:2518	an emended description	2497:2518	an emended description of the genus Ruania	2497:2538	nov., with an emended description of the genus Ruania are proposed.
33835911	11	50	theme	acid	1799:1802	arg1	values					1813:1818	average amino acid identity values	1785:1818	average amino acid identity values	1785:1818	Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales) as a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively.
33835911	0	51	theme	Haloactinobacterium	20:38	arg1	glacieicola					40:50	Haloactinobacterium glacieicola	20:50	Haloactinobacterium glacieicola	20:50	Reclassification of Haloactinobacterium glacieicola as Occultella glacieicola gen. nov., comb.
33835911	9	52	theme	whole-cell	1229:1238	arg1	sugars					1240:1245	Major whole-cell sugars	1223:1245	Major whole-cell sugars	1223:1245	Major whole-cell sugars were rhamnose and ribose.
33835911	6	53	theme	type	795:798	arg1	A11.54					817:822	A11.54	817:822	A11.54	817:822	Its peptidoglycan was of type A4α l-Lys-l-Glu (A11.54).
33835911	6	53	theme	type	795:798	arg1	l-Lys-l-Glu					804:814	type A4α l-Lys-l-Glu	795:814	type A4α l-Lys-l-Glu (A11.54)	795:823	Its peptidoglycan was of type A4α l-Lys-l-Glu (A11.54).
33835911	3	54	theme	ancient	395:401	arg1	smelter					410:416	an ancient copper smelter	392:416	an ancient copper smelter	392:416	nov., a halotolerant isolate from surface soil sampled at an ancient copper smelter.
33835911	2	55	theme	emended	164:170	arg1	description					172:182	an emended description	161:182	an emended description of the genus Ruania	161:202	nov, with an emended description of the genus Ruania, recognition that the genus names Haloactinobacterium and Ruania are heterotypic synonyms and description of Occultella aeris sp.
33835911	11	56	theme	average	1785:1791	arg1	values					1813:1818	average amino acid identity values	1785:1818	average amino acid identity values	1785:1818	Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales) as a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively.
33835911	4	57	dep	smelters	492:499	arg1	Germany					513:519	Germany	513:519	Germany	513:519	In the course of screening the surface soils of ancient copper mines and smelters (East Harz, Germany) an aerobic, non-motile and halotolerant actinobacterium forming small rods or cocci was isolated.
33835911	12	58	theme	Occultella	2023:2032	arg1	nov.					2045:2048	Occultella aeris gen. nov.	2023:2048	the name Occultella aeris gen. nov.	2014:2048	Genotypic and chemotaxonomic analyses support the view that strain F300T (=DSM 108350T=CIP 111667T) is the type strain of a new genus and new species for which the name Occultella aeris gen. nov., sp.
33835911	11	59	theme	genomic	1460:1466	arg1	DNA					1468:1470	its genomic DNA	1456:1470	its genomic DNA	1456:1470	Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales) as a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively.
33835911	0	60	theme	glacieicola	66:76	arg1	comb					89:92	comb	89:92	comb	89:92	Reclassification of Haloactinobacterium glacieicola as Occultella glacieicola gen. nov., comb.
33835911	0	60	theme	glacieicola	66:76	arg1	nov.					83:86	Occultella glacieicola gen. nov.	55:86	Occultella glacieicola gen. nov.	55:86	Reclassification of Haloactinobacterium glacieicola as Occultella glacieicola gen. nov., comb.
33835911	11	61	theme	%	1837:1837	arg1	identity					1772:1779	an average nucleotide identity	1750:1779	an average nucleotide identity	1750:1779	Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales) as a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively.
33835911	11	61	theme	%	1837:1837	arg1	values					1813:1818	average amino acid identity values	1785:1818	average amino acid identity values	1785:1818	Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales) as a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively.
33835911	11	62	theme	genera	1576:1581	arg1	Ruania					1583:1588	genera Ruania	1576:1588	genera Ruania	1576:1588	Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales) as a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively.
33835911	12	63	theme	new	1992:1994	arg1	species					1996:2002	new species	1992:2002	new species	1992:2002	Genotypic and chemotaxonomic analyses support the view that strain F300T (=DSM 108350T=CIP 111667T) is the type strain of a new genus and new species for which the name Occultella aeris gen. nov., sp.
33835911	2	64	theme	sp	330:331	arg1	description					298:308	description	298:308	description of Occultella aeris sp	298:331	nov, with an emended description of the genus Ruania, recognition that the genus names Haloactinobacterium and Ruania are heterotypic synonyms and description of Occultella aeris sp.
33835911	2	64	theme	sp	330:331	arg1	synonyms					285:292	heterotypic synonyms	273:292	heterotypic synonyms	273:292	nov, with an emended description of the genus Ruania, recognition that the genus names Haloactinobacterium and Ruania are heterotypic synonyms and description of Occultella aeris sp.
33835911	2	64	theme	sp	330:331	arg1	names					232:236	the genus names	222:236	the genus names Haloactinobacterium and Ruania	222:267	nov, with an emended description of the genus Ruania, recognition that the genus names Haloactinobacterium and Ruania are heterotypic synonyms and description of Occultella aeris sp.
33835911	7	65	theme	menaquinone	830:840	arg1	profile					842:848	The menaquinone profile	826:848	The menaquinone profile	826:848	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	10	66	theme	fatty	1421:1425	arg1	acids					1427:1431	not fatty acids	1417:1431	not fatty acids	1417:1431	The fatty acid profile contained mainly iso and anteiso branched fatty acids (anteiso-C15 : 0, iso-C14 : 0) and aldehydes/dimethylacetals (i.e. not fatty acids).
33835911	10	66	theme	fatty	1421:1425	arg1	aldehydes/dimethylacetals					1385:1409	aldehydes/dimethylacetals	1385:1409	aldehydes/dimethylacetals (i.e. not fatty acids)	1385:1432	The fatty acid profile contained mainly iso and anteiso branched fatty acids (anteiso-C15 : 0, iso-C14 : 0) and aldehydes/dimethylacetals (i.e. not fatty acids).
33835911	4	67	theme	small	586:590	arg1	rods					592:595	small rods	586:595	small rods	586:595	In the course of screening the surface soils of ancient copper mines and smelters (East Harz, Germany) an aerobic, non-motile and halotolerant actinobacterium forming small rods or cocci was isolated.
33835911	15	68	theme	Occultella	2401:2410	arg1	comb					2424:2427	Occultella glacieicola comb	2401:2427	Occultella glacieicola comb	2401:2427	Consequently, the reclassification of Haloactinobacterium glacieicola as Occultella glacieicola comb.
33835911	17	69	theme	Ruania	2533:2538	arg1	description					2508:2518	an emended description	2497:2518	an emended description of the genus Ruania	2497:2538	nov., with an emended description of the genus Ruania are proposed.
33835911	5	70	theme	strain	624:629	arg1	F300T					642:646	The strain designated F300T	620:646	The strain designated F300T	620:646	The strain designated F300T developed creamy to yellow colonies on tryptone soy agar and grew optimally at 28 °C, pH 7-8 and with 0.5-2 % (m/v) NaCl.
33835911	10	71	theme	fatty	1277:1281	arg1	profile					1288:1294	The fatty acid profile	1273:1294	The fatty acid profile	1273:1294	The fatty acid profile contained mainly iso and anteiso branched fatty acids (anteiso-C15 : 0, iso-C14 : 0) and aldehydes/dimethylacetals (i.e. not fatty acids).
33835911	15	72	theme	glacieicola	2386:2396	arg1	reclassification					2346:2361	the reclassification	2342:2361	the reclassification of Haloactinobacterium glacieicola as Occultella glacieicola comb	2342:2427	Consequently, the reclassification of Haloactinobacterium glacieicola as Occultella glacieicola comb.
33835911	5	73	dep	%	756:756	arg1	m/v					759:761	m/v	759:761	m/v	759:761	The strain designated F300T developed creamy to yellow colonies on tryptone soy agar and grew optimally at 28 °C, pH 7-8 and with 0.5-2 % (m/v) NaCl.
33835911	8	74	theme	phosphatidylinositol	1021:1040	arg1	phospholipids					1162:1174	two phospholipids	1158:1174	two phospholipids	1158:1174	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, mono and diacylated phosphatidylinositol dimannosides, and components that were not fully characterized, including two phospholipids, two glycolipids and an uncharacterized lipid.
33835911	8	74	theme	phosphatidylinositol	1021:1040	arg1	glycolipids					1181:1191	two glycolipids	1177:1191	two glycolipids	1177:1191	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, mono and diacylated phosphatidylinositol dimannosides, and components that were not fully characterized, including two phospholipids, two glycolipids and an uncharacterized lipid.
33835911	8	74	theme	phosphatidylinositol	1021:1040	arg1	dimannosides					1084:1095	phosphatidylinositol, mono and diacylated phosphatidylinositol dimannosides	1021:1095	dimannosides	1084:1095	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, mono and diacylated phosphatidylinositol dimannosides, and components that were not fully characterized, including two phospholipids, two glycolipids and an uncharacterized lipid.
33835911	8	74	theme	phosphatidylinositol	1021:1040	arg1	lipid					1216:1220	an uncharacterized lipid	1197:1220	an uncharacterized lipid	1197:1220	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, mono and diacylated phosphatidylinositol dimannosides, and components that were not fully characterized, including two phospholipids, two glycolipids and an uncharacterized lipid.
33835911	14	75	theme	family	2261:2266	arg1	Ruaniaceae					2268:2277	the family Ruaniaceae	2257:2277	the family Ruaniaceae	2257:2277	Based on revised chemotaxonomic and additional genome based data, it is necessary to discuss and evaluate the results in the light of the classification and nomenclature of members of the family Ruaniaceae, i.e. the genera Haloactinobacterium and Ruania.
33835911	11	76	theme	Sequence	1435:1442	arg1	analysis					1444:1451	Sequence analysis	1435:1451	Sequence analysis of its genomic DNA	1435:1470	Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales) as a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively.
33835911	2	77	theme	Occultella	313:322	arg1	sp					330:331	Occultella aeris sp	313:331	Occultella aeris sp	313:331	nov, with an emended description of the genus Ruania, recognition that the genus names Haloactinobacterium and Ruania are heterotypic synonyms and description of Occultella aeris sp.
33835911	8	78	theme	mono	1043:1046	arg1	phospholipids					1162:1174	two phospholipids	1158:1174	two phospholipids	1158:1174	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, mono and diacylated phosphatidylinositol dimannosides, and components that were not fully characterized, including two phospholipids, two glycolipids and an uncharacterized lipid.
33835911	8	78	theme	mono	1043:1046	arg1	glycolipids					1181:1191	two glycolipids	1177:1191	two glycolipids	1177:1191	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, mono and diacylated phosphatidylinositol dimannosides, and components that were not fully characterized, including two phospholipids, two glycolipids and an uncharacterized lipid.
33835911	8	78	theme	mono	1043:1046	arg1	dimannosides					1084:1095	phosphatidylinositol, mono and diacylated phosphatidylinositol dimannosides	1021:1095	dimannosides	1084:1095	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, mono and diacylated phosphatidylinositol dimannosides, and components that were not fully characterized, including two phospholipids, two glycolipids and an uncharacterized lipid.
33835911	8	78	theme	mono	1043:1046	arg1	lipid					1216:1220	an uncharacterized lipid	1197:1220	an uncharacterized lipid	1197:1220	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, mono and diacylated phosphatidylinositol dimannosides, and components that were not fully characterized, including two phospholipids, two glycolipids and an uncharacterized lipid.
33835911	12	79	theme	new	1978:1980	arg1	genus					1982:1986	a new genus	1976:1986	a new genus	1976:1986	Genotypic and chemotaxonomic analyses support the view that strain F300T (=DSM 108350T=CIP 111667T) is the type strain of a new genus and new species for which the name Occultella aeris gen. nov., sp.
33835911	8	80	theme	diacylated	1052:1061	arg1	phospholipids					1162:1174	two phospholipids	1158:1174	two phospholipids	1158:1174	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, mono and diacylated phosphatidylinositol dimannosides, and components that were not fully characterized, including two phospholipids, two glycolipids and an uncharacterized lipid.
33835911	8	80	theme	diacylated	1052:1061	arg1	glycolipids					1181:1191	two glycolipids	1177:1191	two glycolipids	1177:1191	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, mono and diacylated phosphatidylinositol dimannosides, and components that were not fully characterized, including two phospholipids, two glycolipids and an uncharacterized lipid.
33835911	8	80	theme	diacylated	1052:1061	arg1	dimannosides					1084:1095	phosphatidylinositol, mono and diacylated phosphatidylinositol dimannosides	1021:1095	dimannosides	1084:1095	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, mono and diacylated phosphatidylinositol dimannosides, and components that were not fully characterized, including two phospholipids, two glycolipids and an uncharacterized lipid.
33835911	8	80	theme	diacylated	1052:1061	arg1	lipid					1216:1220	an uncharacterized lipid	1197:1220	an uncharacterized lipid	1197:1220	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, mono and diacylated phosphatidylinositol dimannosides, and components that were not fully characterized, including two phospholipids, two glycolipids and an uncharacterized lipid.
33835911	7	81	theme	MK-8	925:928	arg1	H2					920:921	H2	920:921	H2	920:921	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	7	81	theme	MK-8	925:928	arg1	MK-9					938:941	MK-9	938:941	MK-9(H4)	938:945	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	7	81	theme	MK-8	925:928	arg1	amounts					904:910	minor amounts	898:910	minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4)	898:945	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	7	81	theme	MK-8	925:928	arg1	H4					943:944	H4	943:944	H4	943:944	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	7	81	theme	MK-8	925:928	arg1	MK-8					925:928	MK-8	925:928	MK-8(H6)	925:932	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	7	81	theme	MK-8	925:928	arg1	MK-8					915:918	MK-8	915:918	MK-8(H2)	915:922	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	7	81	theme	MK-8	925:928	arg1	H6					930:931	H6	930:931	H6	930:931	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	16	82	theme	alba	2475:2478	arg1	comb					2480:2483	Ruania alba comb	2468:2483	Ruania alba comb	2468:2483	nov. and Haloactinobacterium album as Ruania alba comb.
33835911	12	83	theme	type	1961:1964	arg1	F300T					1921:1925	strain F300T	1914:1925	strain F300T (=DSM 108350T=CIP 111667T)	1914:1952	Genotypic and chemotaxonomic analyses support the view that strain F300T (=DSM 108350T=CIP 111667T) is the type strain of a new genus and new species for which the name Occultella aeris gen. nov., sp.
33835911	12	83	theme	type	1961:1964	arg1	strain					1966:1971	the type strain	1957:1971	the type strain of a new genus and new species for which the name Occultella aeris gen. nov., sp	1957:2052	Genotypic and chemotaxonomic analyses support the view that strain F300T (=DSM 108350T=CIP 111667T) is the type strain of a new genus and new species for which the name Occultella aeris gen. nov., sp.
33835911	7	84	theme	MK-8	915:918	arg1	H2					920:921	H2	920:921	H2	920:921	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	7	84	theme	MK-8	915:918	arg1	MK-9					938:941	MK-9	938:941	MK-9(H4)	938:945	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	7	84	theme	MK-8	915:918	arg1	amounts					904:910	minor amounts	898:910	minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4)	898:945	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	7	84	theme	MK-8	915:918	arg1	H4					943:944	H4	943:944	H4	943:944	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	7	84	theme	MK-8	915:918	arg1	MK-8					925:928	MK-8	925:928	MK-8(H6)	925:932	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	7	84	theme	MK-8	915:918	arg1	MK-8					915:918	MK-8	915:918	MK-8(H2)	915:922	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	7	84	theme	MK-8	915:918	arg1	H6					930:931	H6	930:931	H6	930:931	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	2	85	theme	genus	191:195	arg1	Ruania					197:202	the genus Ruania	187:202	the genus Ruania	187:202	nov, with an emended description of the genus Ruania, recognition that the genus names Haloactinobacterium and Ruania are heterotypic synonyms and description of Occultella aeris sp.
33835911	12	86	theme	=DSM	1928:1931	arg1	F300T					1921:1925	strain F300T	1914:1925	strain F300T (=DSM 108350T=CIP 111667T)	1914:1952	Genotypic and chemotaxonomic analyses support the view that strain F300T (=DSM 108350T=CIP 111667T) is the type strain of a new genus and new species for which the name Occultella aeris gen. nov., sp.
33835911	12	86	theme	=DSM	1928:1931	arg1	111667T					1945:1951	=DSM 108350T=CIP 111667T	1928:1951	=DSM 108350T=CIP 111667T	1928:1951	Genotypic and chemotaxonomic analyses support the view that strain F300T (=DSM 108350T=CIP 111667T) is the type strain of a new genus and new species for which the name Occultella aeris gen. nov., sp.
33835911	7	87	dep	MK-8	867:870	arg1	III-H4					876:881	III-H4	876:881	III-H4	876:881	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	7	87	dep	MK-8	867:870	arg1	II					872:873	II	872:873	II	872:873	The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4).
33835911	1	88	theme	Ruania	133:138	arg1	comb					145:148	Ruania alba comb	133:148	Ruania alba comb	133:148	nov., of Haloactinobacterium album as Ruania alba comb.
33835911	4	89	theme	mines	482:486	arg1	soils					458:462	the surface soils	446:462	the surface soils of ancient copper mines and smelters (East Harz, Germany)	446:520	In the course of screening the surface soils of ancient copper mines and smelters (East Harz, Germany) an aerobic, non-motile and halotolerant actinobacterium forming small rods or cocci was isolated.
33835911	4	90	theme	smelters	492:499	arg1	soils					458:462	the surface soils	446:462	the surface soils of ancient copper mines and smelters (East Harz, Germany)	446:520	In the course of screening the surface soils of ancient copper mines and smelters (East Harz, Germany) an aerobic, non-motile and halotolerant actinobacterium forming small rods or cocci was isolated.
33835911	10	91	theme	branched	1329:1336	arg1	anteiso-C15 					1351:1362	anteiso-C15 	1351:1362	anteiso-C15 	1351:1362	The fatty acid profile contained mainly iso and anteiso branched fatty acids (anteiso-C15 : 0, iso-C14 : 0) and aldehydes/dimethylacetals (i.e. not fatty acids).
33835911	10	91	theme	branched	1329:1336	arg1	acids					1344:1348	iso and anteiso branched fatty acids	1313:1348	iso and anteiso branched fatty acids (anteiso-C15 : 0, iso-C14 : 0)	1313:1379	The fatty acid profile contained mainly iso and anteiso branched fatty acids (anteiso-C15 : 0, iso-C14 : 0) and aldehydes/dimethylacetals (i.e. not fatty acids).
33835911	8	92	theme	uncharacterized	1200:1214	arg1	lipid					1216:1220	an uncharacterized lipid	1197:1220	an uncharacterized lipid	1197:1220	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, mono and diacylated phosphatidylinositol dimannosides, and components that were not fully characterized, including two phospholipids, two glycolipids and an uncharacterized lipid.
33835911	14	93	theme	based	2127:2131	arg1	data					2133:2136	revised chemotaxonomic and additional genome based data	2082:2136	revised chemotaxonomic and additional genome based data	2082:2136	Based on revised chemotaxonomic and additional genome based data, it is necessary to discuss and evaluate the results in the light of the classification and nomenclature of members of the family Ruaniaceae, i.e. the genera Haloactinobacterium and Ruania.
33835911	11	94	theme	order	1634:1638	arg1	Ruaniaceae					1622:1631	family Ruaniaceae	1615:1631	family Ruaniaceae	1615:1631	Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales) as a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively.
33835911	11	94	theme	order	1634:1638	arg1	Micrococcales					1640:1652	order Micrococcales	1634:1652	order Micrococcales	1634:1652	Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales) as a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively.
33835911	5	95	theme	soy	696:698	arg1	agar					700:703	tryptone soy agar	687:703	tryptone soy agar	687:703	The strain designated F300T developed creamy to yellow colonies on tryptone soy agar and grew optimally at 28 °C, pH 7-8 and with 0.5-2 % (m/v) NaCl.
33835911	14	96	theme	additional	2109:2118	arg1	data					2133:2136	revised chemotaxonomic and additional genome based data	2082:2136	revised chemotaxonomic and additional genome based data	2082:2136	Based on revised chemotaxonomic and additional genome based data, it is necessary to discuss and evaluate the results in the light of the classification and nomenclature of members of the family Ruaniaceae, i.e. the genera Haloactinobacterium and Ruania.
33835911	2	97	theme	aeris	324:328	arg1	sp					330:331	Occultella aeris sp	313:331	Occultella aeris sp	313:331	nov, with an emended description of the genus Ruania, recognition that the genus names Haloactinobacterium and Ruania are heterotypic synonyms and description of Occultella aeris sp.
33835911	11	98	dep	species	1701:1707	arg1	glacieicola					1729:1739	Haloactinobacterium glacieicola	1709:1739	Haloactinobacterium glacieicola	1709:1739	Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales) as a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively.
33835911	4	99	theme	ancient	467:473	arg1	mines					482:486	ancient copper mines	467:486	ancient copper mines	467:486	In the course of screening the surface soils of ancient copper mines and smelters (East Harz, Germany) an aerobic, non-motile and halotolerant actinobacterium forming small rods or cocci was isolated.
33835911	14	100	theme	chemotaxonomic	2090:2103	arg1	data					2133:2136	revised chemotaxonomic and additional genome based data	2082:2136	revised chemotaxonomic and additional genome based data	2082:2136	Based on revised chemotaxonomic and additional genome based data, it is necessary to discuss and evaluate the results in the light of the classification and nomenclature of members of the family Ruaniaceae, i.e. the genera Haloactinobacterium and Ruania.
33835911	5	101	theme	0.5-2 	750:755	arg1	%					756:756	%	756:756	%	756:756	The strain designated F300T developed creamy to yellow colonies on tryptone soy agar and grew optimally at 28 °C, pH 7-8 and with 0.5-2 % (m/v) NaCl.
33835911	12	102	theme	aeris	2034:2038	arg1	nov.					2045:2048	Occultella aeris gen. nov.	2023:2048	the name Occultella aeris gen. nov.	2014:2048	Genotypic and chemotaxonomic analyses support the view that strain F300T (=DSM 108350T=CIP 111667T) is the type strain of a new genus and new species for which the name Occultella aeris gen. nov., sp.
33835911	11	103	theme	nucleotide	1761:1770	arg1	identity					1772:1779	an average nucleotide identity	1750:1779	an average nucleotide identity	1750:1779	Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales) as a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively.
33835911	0	104	theme	glacieicola	40:50	arg1	Reclassification					0:15	Reclassification	0:15	Reclassification of Haloactinobacterium glacieicola as Occultella glacieicola gen. nov., comb.	0:93	Reclassification of Haloactinobacterium glacieicola as Occultella glacieicola gen. nov., comb.
33835911	9	105	theme	Major	1223:1227	arg1	sugars					1240:1245	Major whole-cell sugars	1223:1245	Major whole-cell sugars	1223:1245	Major whole-cell sugars were rhamnose and ribose.
33835911	3	106	theme	surface	368:374	arg1	soil					376:379	surface soil	368:379	surface soil	368:379	nov., a halotolerant isolate from surface soil sampled at an ancient copper smelter.
33835911	4	107	theme	aerobic	525:531	arg1	actinobacterium					562:576	an aerobic, non-motile and halotolerant actinobacterium	522:576	an aerobic, non-motile and halotolerant actinobacterium forming small rods or cocci	522:604	In the course of screening the surface soils of ancient copper mines and smelters (East Harz, Germany) an aerobic, non-motile and halotolerant actinobacterium forming small rods or cocci was isolated.
33835911	11	108	theme	identity	1804:1811	arg1	values					1813:1818	average amino acid identity values	1785:1818	average amino acid identity values	1785:1818	Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales) as a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively.
33835911	0	109	theme	Occultella	55:64	arg1	comb					89:92	comb	89:92	comb	89:92	Reclassification of Haloactinobacterium glacieicola as Occultella glacieicola gen. nov., comb.
33835911	0	109	theme	Occultella	55:64	arg1	nov.					83:86	Occultella glacieicola gen. nov.	55:86	Occultella glacieicola gen. nov.	55:86	Reclassification of Haloactinobacterium glacieicola as Occultella glacieicola gen. nov., comb.
33835911	3	110	theme	copper	403:408	arg1	smelter					410:416	an ancient copper smelter	392:416	an ancient copper smelter	392:416	nov., a halotolerant isolate from surface soil sampled at an ancient copper smelter.
33835911	11	111	theme	amino	1793:1797	arg1	values					1813:1818	average amino acid identity values	1785:1818	average amino acid identity values	1785:1818	Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales) as a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively.
33835911	0	112	theme	gen.	78:81	arg1	comb					89:92	comb	89:92	comb	89:92	Reclassification of Haloactinobacterium glacieicola as Occultella glacieicola gen. nov., comb.
33835911	0	112	theme	gen.	78:81	arg1	nov.					83:86	Occultella glacieicola gen. nov.	55:86	Occultella glacieicola gen. nov.	55:86	Reclassification of Haloactinobacterium glacieicola as Occultella glacieicola gen. nov., comb.
33835911	14	113	dep	Haloactinobacterium	2296:2314	arg1	i.e.					2280:2283	i.e.	2280:2283	i.e.	2280:2283	Based on revised chemotaxonomic and additional genome based data, it is necessary to discuss and evaluate the results in the light of the classification and nomenclature of members of the family Ruaniaceae, i.e. the genera Haloactinobacterium and Ruania.
33835911	2	114	theme	Ruania	197:202	arg1	description					172:182	an emended description	161:182	an emended description of the genus Ruania	161:202	nov, with an emended description of the genus Ruania, recognition that the genus names Haloactinobacterium and Ruania are heterotypic synonyms and description of Occultella aeris sp.
33835911	8	115	theme	polar	952:956	arg1	lipids					958:963	The polar lipids	948:963	The polar lipids	948:963	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, mono and diacylated phosphatidylinositol dimannosides, and components that were not fully characterized, including two phospholipids, two glycolipids and an uncharacterized lipid.
33835911	11	116	theme	subsequent	1476:1485	arg1	analysis					1487:1494	subsequent analysis	1476:1494	subsequent analysis of the data	1476:1506	Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales) as a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively.
33835911	11	117	theme	described	1691:1699	arg1	species					1701:1707	the previously described species	1676:1707	the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively	1676:1851	Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales) as a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively.
33835911	15	118	theme	glacieicola	2412:2422	arg1	comb					2424:2427	Occultella glacieicola comb	2401:2427	Occultella glacieicola comb	2401:2427	Consequently, the reclassification of Haloactinobacterium glacieicola as Occultella glacieicola comb.
33835911	11	119	theme	data	1503:1506	arg1	analysis					1444:1451	Sequence analysis	1435:1451	Sequence analysis of its genomic DNA	1435:1470	Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales) as a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively.
33835911	11	119	theme	data	1503:1506	arg1	analysis					1487:1494	subsequent analysis	1476:1494	subsequent analysis of the data	1476:1506	Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales) as a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively.
33835911	12	120	theme	species	1996:2002	arg1	F300T					1921:1925	strain F300T	1914:1925	strain F300T (=DSM 108350T=CIP 111667T)	1914:1952	Genotypic and chemotaxonomic analyses support the view that strain F300T (=DSM 108350T=CIP 111667T) is the type strain of a new genus and new species for which the name Occultella aeris gen. nov., sp.
33835911	12	120	theme	species	1996:2002	arg1	strain					1966:1971	the type strain	1957:1971	the type strain of a new genus and new species for which the name Occultella aeris gen. nov., sp	1957:2052	Genotypic and chemotaxonomic analyses support the view that strain F300T (=DSM 108350T=CIP 111667T) is the type strain of a new genus and new species for which the name Occultella aeris gen. nov., sp.
33835911	11	121	theme	DNA	1468:1470	arg1	analysis					1444:1451	Sequence analysis	1435:1451	Sequence analysis of its genomic DNA	1435:1470	Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales) as a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively.
33835911	11	121	theme	DNA	1468:1470	arg1	analysis					1487:1494	subsequent analysis	1476:1494	subsequent analysis of the data	1476:1506	Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae, order Micrococcales) as a sister taxon to the previously described species Haloactinobacterium glacieicola, sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively.
32806962	0	0	theme	nodes	174:178	arg1	cells					180:184	spleen and lymph nodes cells	157:184	spleen and lymph nodes cells	157:184	Immunotoxicological effects triggered by the rattlesnake Crotalus durissus cumanensis, mapanare (Bothrops colombiensis) venoms and its purified fractions on spleen and lymph nodes cells.
32806962	1	1	theme	venom	244:248	arg1	composition					250:260	their different venom composition	228:260	their different venom composition	228:260	Purpose: The snakes in Venezuela vary in their different venom composition amid the species.
32806962	2	2	theme	Bothrops	582:589	arg1	colombiensis					591:602	Bothrops colombiensis	582:602	Bothrops colombiensis	582:602	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	2	2	theme	Bothrops	582:589	arg1	venoms					611:616	(Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms	531:616	venoms	611:616	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	9	3	dep	persists	1756:1763	arg1	controlled					1765:1774	controlled	1765:1774	persists controlled	1756:1774	As it is known, lymphocytes are cells of enormous flexibility and can operate in diverse aspects, warranting that the correct immune response persists controlled.
32806962	9	4	theme	immune	1740:1745	arg1	response					1747:1754	the correct immune response	1728:1754	the correct immune response	1728:1754	As it is known, lymphocytes are cells of enormous flexibility and can operate in diverse aspects, warranting that the correct immune response persists controlled.
32806962	2	5	theme	venoms	611:616	arg1	actions					618:624	the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions	515:624	the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions	515:624	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	4	6	theme	Crotalus	1000:1007	arg1	venom					1015:1019	the Crotalus crude venom	996:1019	the Crotalus crude venom	996:1019	Results: The lymphocyte response was measured by the Alamar Blue® (Resazurin) assay, observing that the Crotalus crude venom increased basal proliferation in the spleen and lymph nodes, being also increased with ConA and LPS.
32806962	6	7	theme	SPF	1223:1225	arg1	fraction					1227:1234	its SPF fraction	1219:1234	its SPF fraction	1219:1234	Both Bc venom and its SPF fraction had no significant effect on basal proliferation in the spleen and lymph nodes, but a decrease in the response with ConA was observed.
32806962	3	8	dep	Material	731:738	arg1	carried					801:807	carried	801:807	was carried out in the presence or absence of Concanavalin A (ConA) and lipopolysaccharides (LPS)	797:893	Material and methods: The stimulation of lymphocyte proliferation was carried out in the presence or absence of Concanavalin A (ConA) and lipopolysaccharides (LPS).
32806962	0	9	theme	purified	135:142	arg1	fractions					144:152	its purified fractions	131:152	its purified fractions	131:152	Immunotoxicological effects triggered by the rattlesnake Crotalus durissus cumanensis, mapanare (Bothrops colombiensis) venoms and its purified fractions on spleen and lymph nodes cells.
32806962	1	10	from	snakes	200:205	arg1	Venezuela					210:218	Venezuela	210:218	Venezuela	210:218	Purpose: The snakes in Venezuela vary in their different venom composition amid the species.
32806962	7	11	contain	has	1402:1404	arg1	CTX					1398:1400	CTX	1398:1400	CTX	1398:1400	These results suggest that CTX has an inhibitory action on lymphocyte proliferation, while Cdc crude venom has a stimulatory action on T and B cell populations.
32806962	7	11	contain	has	1402:1404	arg2	action					1420:1425	an inhibitory action	1406:1425	an inhibitory action on lymphocyte proliferation	1406:1453	These results suggest that CTX has an inhibitory action on lymphocyte proliferation, while Cdc crude venom has a stimulatory action on T and B cell populations.
32806962	2	12	theme	mapanare	572:579	arg1	colombiensis					591:602	Bothrops colombiensis	582:602	Bothrops colombiensis	582:602	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	2	12	theme	mapanare	572:579	arg1	venoms					611:616	(Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms	531:616	venoms	611:616	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	1	13	dep	vary	220:223	arg1	amid					262:265	amid	262:265	vary in their different venom composition amid the species	220:277	Purpose: The snakes in Venezuela vary in their different venom composition amid the species.
32806962	10	14	theme	different	1825:1833	arg1	toxins					1835:1840	these different toxins	1819:1840	these different toxins	1819:1840	Conclusions: These results suggested that these different toxins can modulate lymphocyte functional activation toward an inhibitory or stimulatory state.
32806962	6	15	theme	Bc	1206:1207	arg1	venom					1209:1213	Bc venom	1206:1213	Bc venom	1206:1213	Both Bc venom and its SPF fraction had no significant effect on basal proliferation in the spleen and lymph nodes, but a decrease in the response with ConA was observed.
32806962	3	16	theme	proliferation	783:795	arg1	stimulation					757:767	The stimulation	753:767	The stimulation of lymphocyte proliferation	753:795	Material and methods: The stimulation of lymphocyte proliferation was carried out in the presence or absence of Concanavalin A (ConA) and lipopolysaccharides (LPS).
32806962	7	17	theme	Cdc	1462:1464	arg1	venom					1472:1476	Cdc crude venom	1462:1476	Cdc crude venom	1462:1476	These results suggest that CTX has an inhibitory action on lymphocyte proliferation, while Cdc crude venom has a stimulatory action on T and B cell populations.
32806962	2	18	theme	semi-purified	685:697	arg1	SPF					709:711	SPF	709:711	SPF	709:711	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	2	18	theme	semi-purified	685:697	arg1	Bc					715:716	Bc	715:716	Bc	715:716	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	2	18	theme	semi-purified	685:697	arg1	fraction					699:706	a semi-purified fraction	683:706	a semi-purified fraction (SPF) (Bc)	683:717	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	5	19	theme	mitogens	1191:1198	arg1	presence					1179:1186	the presence	1175:1186	the presence of mitogens	1175:1198	CTX slightly decreased the proliferative response in the presence of mitogens.
32806962	10	20	theme	lymphocyte	1855:1864	arg1	activation					1877:1886	lymphocyte functional activation	1855:1886	lymphocyte functional activation	1855:1886	Conclusions: These results suggested that these different toxins can modulate lymphocyte functional activation toward an inhibitory or stimulatory state.
32806962	2	21	from	inhibition	436:445	arg1	spleen					474:479	the mice spleen	465:479	the mice spleen	465:479	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	2	21	from	inhibition	436:445	arg1	nodes					491:495	lymph nodes	485:495	lymph nodes	485:495	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	4	22	theme	lymph	1069:1073	arg1	nodes					1075:1079	lymph nodes	1069:1079	lymph nodes	1069:1079	Results: The lymphocyte response was measured by the Alamar Blue® (Resazurin) assay, observing that the Crotalus crude venom increased basal proliferation in the spleen and lymph nodes, being also increased with ConA and LPS.
32806962	5	23	theme	proliferative	1149:1161	arg1	response					1163:1170	the proliferative response	1145:1170	the proliferative response	1145:1170	CTX slightly decreased the proliferative response in the presence of mitogens.
32806962	7	24	theme	lymphocyte	1430:1439	arg1	proliferation					1441:1453	lymphocyte proliferation	1430:1453	lymphocyte proliferation	1430:1453	These results suggest that CTX has an inhibitory action on lymphocyte proliferation, while Cdc crude venom has a stimulatory action on T and B cell populations.
32806962	2	25	from	stimulation	450:460	arg1	spleen					474:479	the mice spleen	465:479	the mice spleen	465:479	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	2	25	from	stimulation	450:460	arg1	nodes					491:495	lymph nodes	485:495	lymph nodes	485:495	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	0	26	theme	Bothrops	97:104	arg1	venoms					120:125	the rattlesnake Crotalus durissus cumanensis, mapanare (Bothrops colombiensis) venoms	41:125	the rattlesnake Crotalus durissus cumanensis, mapanare (Bothrops colombiensis) venoms	41:125	Immunotoxicological effects triggered by the rattlesnake Crotalus durissus cumanensis, mapanare (Bothrops colombiensis) venoms and its purified fractions on spleen and lymph nodes cells.
32806962	2	27	dep	spleen	474:479	arg1	lymphocytes					497:507	lymphocytes	497:507	lymphocytes	497:507	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	3	28	theme	Concanavalin	843:854	arg1	ConA					859:862	ConA	859:862	ConA	859:862	Material and methods: The stimulation of lymphocyte proliferation was carried out in the presence or absence of Concanavalin A (ConA) and lipopolysaccharides (LPS).
32806962	3	28	theme	Concanavalin	843:854	arg1	A					856:856	Concanavalin A	843:856	Concanavalin A (ConA)	843:863	Material and methods: The stimulation of lymphocyte proliferation was carried out in the presence or absence of Concanavalin A (ConA) and lipopolysaccharides (LPS).
32806962	2	29	theme	rattlesnake	519:529	arg1	actions					618:624	the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions	515:624	the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions	515:624	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	2	30	theme	immunosuppressive	390:406	arg1	effects					408:414	immunosuppressive effects	390:414	immunosuppressive effects	390:414	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	8	31	contain	had	1560:1562	arg2	effect					1567:1572	no effect	1564:1572	no effect	1564:1572	Bothrops colombiensis venom had no effect on these two types of cell populations.
32806962	8	31	contain	had	1560:1562	arg1	venom					1554:1558	Bothrops colombiensis venom	1532:1558	Bothrops colombiensis venom	1532:1558	Bothrops colombiensis venom had no effect on these two types of cell populations.
32806962	0	32	theme	rattlesnake	45:55	arg1	venoms					120:125	the rattlesnake Crotalus durissus cumanensis, mapanare (Bothrops colombiensis) venoms	41:125	the rattlesnake Crotalus durissus cumanensis, mapanare (Bothrops colombiensis) venoms	41:125	Immunotoxicological effects triggered by the rattlesnake Crotalus durissus cumanensis, mapanare (Bothrops colombiensis) venoms and its purified fractions on spleen and lymph nodes cells.
32806962	0	33	dep	Bothrops	97:104	arg1	colombiensis					106:117	Bothrops colombiensis	97:117	the rattlesnake Crotalus durissus cumanensis, mapanare (Bothrops colombiensis) venoms	41:125	Immunotoxicological effects triggered by the rattlesnake Crotalus durissus cumanensis, mapanare (Bothrops colombiensis) venoms and its purified fractions on spleen and lymph nodes cells.
32806962	0	34	theme	Immunotoxicological	0:18	arg1	effects					20:26	Immunotoxicological effects	0:26	Immunotoxicological effects	0:26	Immunotoxicological effects triggered by the rattlesnake Crotalus durissus cumanensis, mapanare (Bothrops colombiensis) venoms and its purified fractions on spleen and lymph nodes cells.
32806962	3	35	dep	presence	820:827	arg1	the					816:818	the	816:818	the	816:818	Material and methods: The stimulation of lymphocyte proliferation was carried out in the presence or absence of Concanavalin A (ConA) and lipopolysaccharides (LPS).
32806962	7	36	theme	cell	1514:1517	arg1	populations					1519:1529	cell populations	1514:1529	cell populations	1514:1529	These results suggest that CTX has an inhibitory action on lymphocyte proliferation, while Cdc crude venom has a stimulatory action on T and B cell populations.
32806962	4	37	theme	Blue®	956:960	arg1	assay					974:978	the Alamar Blue® (Resazurin) assay	945:978	the Alamar Blue® (Resazurin) assay	945:978	Results: The lymphocyte response was measured by the Alamar Blue® (Resazurin) assay, observing that the Crotalus crude venom increased basal proliferation in the spleen and lymph nodes, being also increased with ConA and LPS.
32806962	2	38	theme	fraction	649:656	arg1	Cdc					674:676	Cdc	674:676	Cdc	674:676	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	2	38	theme	fraction	649:656	arg1	crotoxin					658:665	its purified fraction crotoxin	636:665	its purified fraction crotoxin (CTX) (Cdc)	636:677	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	2	38	theme	fraction	649:656	arg1	CTX					668:670	CTX	668:670	CTX	668:670	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	2	39	theme	measuring	426:434	arg1	activities					719:728	in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities	416:728	in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities	416:728	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	7	40	from	action	1420:1425	arg1	proliferation					1441:1453	lymphocyte proliferation	1430:1453	lymphocyte proliferation	1430:1453	These results suggest that CTX has an inhibitory action on lymphocyte proliferation, while Cdc crude venom has a stimulatory action on T and B cell populations.
32806962	9	41	theme	enormous	1655:1662	arg1	flexibility					1664:1674	enormous flexibility	1655:1674	enormous flexibility	1655:1674	As it is known, lymphocytes are cells of enormous flexibility and can operate in diverse aspects, warranting that the correct immune response persists controlled.
32806962	4	42	theme	basal	1031:1035	arg1	proliferation					1037:1049	basal proliferation	1031:1049	basal proliferation	1031:1049	Results: The lymphocyte response was measured by the Alamar Blue® (Resazurin) assay, observing that the Crotalus crude venom increased basal proliferation in the spleen and lymph nodes, being also increased with ConA and LPS.
32806962	2	43	theme	in vitro	416:423	arg1	activities					719:728	in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities	416:728	in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities	416:728	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	4	44	theme	lymphocyte	909:918	arg1	response					920:927	The lymphocyte response	905:927	The lymphocyte response	905:927	Results: The lymphocyte response was measured by the Alamar Blue® (Resazurin) assay, observing that the Crotalus crude venom increased basal proliferation in the spleen and lymph nodes, being also increased with ConA and LPS.
32806962	0	45	dep	Crotalus	57:64	arg1	mapanare					87:94	mapanare	87:94	mapanare	87:94	Immunotoxicological effects triggered by the rattlesnake Crotalus durissus cumanensis, mapanare (Bothrops colombiensis) venoms and its purified fractions on spleen and lymph nodes cells.
32806962	0	45	dep	Crotalus	57:64	arg1	cumanensis					75:84	Crotalus durissus cumanensis, mapanare	57:94	cumanensis	75:84	Immunotoxicological effects triggered by the rattlesnake Crotalus durissus cumanensis, mapanare (Bothrops colombiensis) venoms and its purified fractions on spleen and lymph nodes cells.
32806962	2	46	theme	mice	469:472	arg1	spleen					474:479	the mice spleen	465:479	the mice spleen	465:479	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	0	47	theme	Crotalus	57:64	arg1	venoms					120:125	the rattlesnake Crotalus durissus cumanensis, mapanare (Bothrops colombiensis) venoms	41:125	the rattlesnake Crotalus durissus cumanensis, mapanare (Bothrops colombiensis) venoms	41:125	Immunotoxicological effects triggered by the rattlesnake Crotalus durissus cumanensis, mapanare (Bothrops colombiensis) venoms and its purified fractions on spleen and lymph nodes cells.
32806962	3	48	theme	lipopolysaccharides	869:887	arg1	absence					832:838	absence	832:838	absence	832:838	Material and methods: The stimulation of lymphocyte proliferation was carried out in the presence or absence of Concanavalin A (ConA) and lipopolysaccharides (LPS).
32806962	3	48	theme	lipopolysaccharides	869:887	arg1	presence					820:827	presence	820:827	presence	820:827	Material and methods: The stimulation of lymphocyte proliferation was carried out in the presence or absence of Concanavalin A (ConA) and lipopolysaccharides (LPS).
32806962	2	49	dep	immunostimulatory	365:381	arg1	activities					719:728	in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities	416:728	in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities	416:728	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	6	50	from	decrease	1322:1329	arg1	response					1338:1345	the response	1334:1345	the response with ConA	1334:1355	Both Bc venom and its SPF fraction had no significant effect on basal proliferation in the spleen and lymph nodes, but a decrease in the response with ConA was observed.
32806962	9	51	theme	diverse	1695:1701	arg1	aspects					1703:1709	diverse aspects	1695:1709	diverse aspects	1695:1709	As it is known, lymphocytes are cells of enormous flexibility and can operate in diverse aspects, warranting that the correct immune response persists controlled.
32806962	4	52	theme	Resazurin	963:971	arg1	assay					974:978	the Alamar Blue® (Resazurin) assay	945:978	the Alamar Blue® (Resazurin) assay	945:978	Results: The lymphocyte response was measured by the Alamar Blue® (Resazurin) assay, observing that the Crotalus crude venom increased basal proliferation in the spleen and lymph nodes, being also increased with ConA and LPS.
32806962	1	53	theme	different	234:242	arg1	composition					250:260	their different venom composition	228:260	their different venom composition	228:260	Purpose: The snakes in Venezuela vary in their different venom composition amid the species.
32806962	8	54	theme	colombiensis	1541:1552	arg1	venom					1554:1558	Bothrops colombiensis venom	1532:1558	Bothrops colombiensis venom	1532:1558	Bothrops colombiensis venom had no effect on these two types of cell populations.
32806962	9	55	theme	correct	1732:1738	arg1	response					1747:1754	the correct immune response	1728:1754	the correct immune response	1728:1754	As it is known, lymphocytes are cells of enormous flexibility and can operate in diverse aspects, warranting that the correct immune response persists controlled.
32806962	6	56	contain	had	1236:1238	arg1	venom					1209:1213	Bc venom	1206:1213	Bc venom	1206:1213	Both Bc venom and its SPF fraction had no significant effect on basal proliferation in the spleen and lymph nodes, but a decrease in the response with ConA was observed.
32806962	6	56	contain	had	1236:1238	arg2	effect					1255:1260	no significant effect	1240:1260	no significant effect	1240:1260	Both Bc venom and its SPF fraction had no significant effect on basal proliferation in the spleen and lymph nodes, but a decrease in the response with ConA was observed.
32806962	6	56	contain	had	1236:1238	arg1	fraction					1227:1234	its SPF fraction	1219:1234	its SPF fraction	1219:1234	Both Bc venom and its SPF fraction had no significant effect on basal proliferation in the spleen and lymph nodes, but a decrease in the response with ConA was observed.
32806962	6	57	with	response	1338:1345	arg1	ConA					1352:1355	ConA	1352:1355	ConA	1352:1355	Both Bc venom and its SPF fraction had no significant effect on basal proliferation in the spleen and lymph nodes, but a decrease in the response with ConA was observed.
32806962	4	58	dep	spleen	1058:1063	arg1	the					1054:1056	the	1054:1056	the	1054:1056	Results: The lymphocyte response was measured by the Alamar Blue® (Resazurin) assay, observing that the Crotalus crude venom increased basal proliferation in the spleen and lymph nodes, being also increased with ConA and LPS.
32806962	2	59	theme	crude	605:609	arg1	colombiensis					591:602	Bothrops colombiensis	582:602	Bothrops colombiensis	582:602	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	2	59	theme	crude	605:609	arg1	venoms					611:616	(Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms	531:616	venoms	611:616	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	4	60	theme	crude	1009:1013	arg1	venom					1015:1019	the Crotalus crude venom	996:1019	the Crotalus crude venom	996:1019	Results: The lymphocyte response was measured by the Alamar Blue® (Resazurin) assay, observing that the Crotalus crude venom increased basal proliferation in the spleen and lymph nodes, being also increased with ConA and LPS.
32806962	7	61	theme	stimulatory	1484:1494	arg1	action					1496:1501	a stimulatory action	1482:1501	a stimulatory action on T and B cell populations	1482:1529	These results suggest that CTX has an inhibitory action on lymphocyte proliferation, while Cdc crude venom has a stimulatory action on T and B cell populations.
32806962	10	62	theme	inhibitory	1898:1907	arg1	state					1924:1928	an inhibitory or stimulatory state	1895:1928	an inhibitory or stimulatory state	1895:1928	Conclusions: These results suggested that these different toxins can modulate lymphocyte functional activation toward an inhibitory or stimulatory state.
32806962	0	63	theme	spleen	157:162	arg1	cells					180:184	spleen and lymph nodes cells	157:184	spleen and lymph nodes cells	157:184	Immunotoxicological effects triggered by the rattlesnake Crotalus durissus cumanensis, mapanare (Bothrops colombiensis) venoms and its purified fractions on spleen and lymph nodes cells.
32806962	8	64	theme	cell	1596:1599	arg1	populations					1601:1611	cell populations	1596:1611	cell populations	1596:1611	Bothrops colombiensis venom had no effect on these two types of cell populations.
32806962	7	65	dep	T	1506:1506	arg1	populations					1519:1529	cell populations	1514:1529	cell populations	1514:1529	These results suggest that CTX has an inhibitory action on lymphocyte proliferation, while Cdc crude venom has a stimulatory action on T and B cell populations.
32806962	7	66	theme	crude	1466:1470	arg1	venom					1472:1476	Cdc crude venom	1462:1476	Cdc crude venom	1462:1476	These results suggest that CTX has an inhibitory action on lymphocyte proliferation, while Cdc crude venom has a stimulatory action on T and B cell populations.
32806962	0	67	from	venoms	120:125	arg1	cells					180:184	spleen and lymph nodes cells	157:184	spleen and lymph nodes cells	157:184	Immunotoxicological effects triggered by the rattlesnake Crotalus durissus cumanensis, mapanare (Bothrops colombiensis) venoms and its purified fractions on spleen and lymph nodes cells.
32806962	0	68	theme	lymph	168:172	arg1	nodes					174:178	lymph nodes	168:178	lymph nodes	168:178	Immunotoxicological effects triggered by the rattlesnake Crotalus durissus cumanensis, mapanare (Bothrops colombiensis) venoms and its purified fractions on spleen and lymph nodes cells.
32806962	2	69	theme	Cdc	563:565	arg1	actions					618:624	the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions	515:624	the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions	515:624	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	10	70	theme	functional	1866:1875	arg1	activation					1877:1886	lymphocyte functional activation	1855:1886	lymphocyte functional activation	1855:1886	Conclusions: These results suggested that these different toxins can modulate lymphocyte functional activation toward an inhibitory or stimulatory state.
32806962	6	71	theme	significant	1243:1253	arg1	effect					1255:1260	no significant effect	1240:1260	no significant effect	1240:1260	Both Bc venom and its SPF fraction had no significant effect on basal proliferation in the spleen and lymph nodes, but a decrease in the response with ConA was observed.
32806962	6	72	theme	basal	1265:1269	arg1	proliferation					1271:1283	basal proliferation	1265:1283	basal proliferation in the spleen and lymph nodes	1265:1313	Both Bc venom and its SPF fraction had no significant effect on basal proliferation in the spleen and lymph nodes, but a decrease in the response with ConA was observed.
32806962	2	73	theme	lymph	485:489	arg1	nodes					491:495	lymph nodes	485:495	lymph nodes	485:495	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	2	74	theme	purified	640:647	arg1	Cdc					674:676	Cdc	674:676	Cdc	674:676	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	2	74	theme	purified	640:647	arg1	crotoxin					658:665	its purified fraction crotoxin	636:665	its purified fraction crotoxin (CTX) (Cdc)	636:677	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	2	74	theme	purified	640:647	arg1	CTX					668:670	CTX	668:670	CTX	668:670	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	3	75	theme	lymphocyte	772:781	arg1	proliferation					783:795	lymphocyte proliferation	772:795	lymphocyte proliferation	772:795	Material and methods: The stimulation of lymphocyte proliferation was carried out in the presence or absence of Concanavalin A (ConA) and lipopolysaccharides (LPS).
32806962	6	76	from	proliferation	1271:1283	arg1	spleen					1292:1297	spleen	1292:1297	spleen	1292:1297	Both Bc venom and its SPF fraction had no significant effect on basal proliferation in the spleen and lymph nodes, but a decrease in the response with ConA was observed.
32806962	6	76	from	proliferation	1271:1283	arg1	nodes					1309:1313	lymph nodes	1303:1313	lymph nodes	1303:1313	Both Bc venom and its SPF fraction had no significant effect on basal proliferation in the spleen and lymph nodes, but a decrease in the response with ConA was observed.
32806962	7	77	theme	inhibitory	1409:1418	arg1	action					1420:1425	an inhibitory action	1406:1425	an inhibitory action on lymphocyte proliferation	1406:1453	These results suggest that CTX has an inhibitory action on lymphocyte proliferation, while Cdc crude venom has a stimulatory action on T and B cell populations.
32806962	3	78	theme	A	856:856	arg1	absence					832:838	absence	832:838	absence	832:838	Material and methods: The stimulation of lymphocyte proliferation was carried out in the presence or absence of Concanavalin A (ConA) and lipopolysaccharides (LPS).
32806962	3	78	theme	A	856:856	arg1	presence					820:827	presence	820:827	presence	820:827	Material and methods: The stimulation of lymphocyte proliferation was carried out in the presence or absence of Concanavalin A (ConA) and lipopolysaccharides (LPS).
32806962	2	79	theme	crotoxin	658:665	arg1	activities					719:728	in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities	416:728	in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities	416:728	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	6	80	dep	spleen	1292:1297	arg1	the					1288:1290	the	1288:1290	the	1288:1290	Both Bc venom and its SPF fraction had no significant effect on basal proliferation in the spleen and lymph nodes, but a decrease in the response with ConA was observed.
32806962	4	81	theme	Alamar	949:954	arg1	assay					974:978	the Alamar Blue® (Resazurin) assay	945:978	the Alamar Blue® (Resazurin) assay	945:978	Results: The lymphocyte response was measured by the Alamar Blue® (Resazurin) assay, observing that the Crotalus crude venom increased basal proliferation in the spleen and lymph nodes, being also increased with ConA and LPS.
32806962	6	82	theme	lymph	1303:1307	arg1	nodes					1309:1313	lymph nodes	1303:1313	lymph nodes	1303:1313	Both Bc venom and its SPF fraction had no significant effect on basal proliferation in the spleen and lymph nodes, but a decrease in the response with ConA was observed.
32806962	2	83	dep	Crotalus	532:539	arg1	cumanensis					550:559	Crotalus durissus cumanensis	532:559	Crotalus durissus cumanensis	532:559	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	9	84	theme	flexibility	1664:1674	arg1	cells					1646:1650	cells	1646:1650	cells of enormous flexibility	1646:1674	As it is known, lymphocytes are cells of enormous flexibility and can operate in diverse aspects, warranting that the correct immune response persists controlled.
32806962	9	84	theme	flexibility	1664:1674	arg1	lymphocytes					1630:1640	lymphocytes	1630:1640	lymphocytes	1630:1640	As it is known, lymphocytes are cells of enormous flexibility and can operate in diverse aspects, warranting that the correct immune response persists controlled.
32806962	7	85	from	action	1496:1501	arg1	B					1512:1512	B	1512:1512	B	1512:1512	These results suggest that CTX has an inhibitory action on lymphocyte proliferation, while Cdc crude venom has a stimulatory action on T and B cell populations.
32806962	7	85	from	action	1496:1501	arg1	T					1506:1506	T	1506:1506	T	1506:1506	These results suggest that CTX has an inhibitory action on lymphocyte proliferation, while Cdc crude venom has a stimulatory action on T and B cell populations.
32806962	0	86	from	fractions	144:152	arg1	cells					180:184	spleen and lymph nodes cells	157:184	spleen and lymph nodes cells	157:184	Immunotoxicological effects triggered by the rattlesnake Crotalus durissus cumanensis, mapanare (Bothrops colombiensis) venoms and its purified fractions on spleen and lymph nodes cells.
32806962	7	87	contain	has	1478:1480	arg2	action					1496:1501	a stimulatory action	1482:1501	a stimulatory action on T and B cell populations	1482:1529	These results suggest that CTX has an inhibitory action on lymphocyte proliferation, while Cdc crude venom has a stimulatory action on T and B cell populations.
32806962	7	87	contain	has	1478:1480	arg1	venom					1472:1476	Cdc crude venom	1462:1476	Cdc crude venom	1462:1476	These results suggest that CTX has an inhibitory action on lymphocyte proliferation, while Cdc crude venom has a stimulatory action on T and B cell populations.
32806962	8	88	theme	populations	1601:1611	arg1	types					1587:1591	these two types	1577:1591	these two types of cell populations	1577:1611	Bothrops colombiensis venom had no effect on these two types of cell populations.
32806962	10	89	theme	stimulatory	1912:1922	arg1	state					1924:1928	an inhibitory or stimulatory state	1895:1928	an inhibitory or stimulatory state	1895:1928	Conclusions: These results suggested that these different toxins can modulate lymphocyte functional activation toward an inhibitory or stimulatory state.
32806962	2	90	theme	related	348:354	arg1	mechanisms					337:346	mechanisms	337:346	mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities	337:728	In this sense, studies have been carried out elucidating mechanisms related to their immunostimulatory and/or immunosuppressive effects in vitro, measuring inhibition or stimulation on the mice spleen and lymph nodes lymphocytes under the rattlesnake (Crotalus durissus cumanensis) (Cdc) and mapanare (Bothrops colombiensis) crude venoms actions, and also its purified fraction crotoxin (CTX) (Cdc) and a semi-purified fraction (SPF) (Bc) activities.
32806962	8	91	theme	Bothrops	1532:1539	arg1	venom					1554:1558	Bothrops colombiensis venom	1532:1558	Bothrops colombiensis venom	1532:1558	Bothrops colombiensis venom had no effect on these two types of cell populations.
31926226	7	0	theme	gut	1059:1061	arg1	microbiota					1063:1072	gut microbiota	1059:1072	gut microbiota	1059:1072	Moreover, we discovered that carrageenan-induced colitis in high-fat diet model was robustly correlated with changes in the composition of gut microbiota, specifically Alistipes finegoldii and Bacteroides acidifaciens.
31926226	5	1	theme	activity	661:668	arg1	DAI					677:679	DAI	677:679	DAI	677:679	We found that in high-fat diet model, native carrageenan in drinking water increased the disease activity index (DAI), myeloperoxidase (MPO) activity and the mRNA expression of TLR4 in colon, whereas carrageenan-supplemented diet has no visible effects.
31926226	5	1	theme	activity	661:668	arg1	index					670:674	the disease activity index	649:674	the disease activity index (DAI)	649:680	We found that in high-fat diet model, native carrageenan in drinking water increased the disease activity index (DAI), myeloperoxidase (MPO) activity and the mRNA expression of TLR4 in colon, whereas carrageenan-supplemented diet has no visible effects.
31926226	1	2	theme	ulcerative	145:154	arg1	colitis					156:162	ulcerative colitis	145:162	ulcerative colitis	145:162	Inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis, has gradually emerged as a public health challenge worldwide.
31926226	6	3	theme	low-fat	867:873	arg1	diet					875:878	low-fat diet	867:878	low-fat diet	867:878	However, no signs of colitis were observed under low-fat diet regardless of the mode of vehicle used.
31926226	3	4	from	exacerbation	378:389	arg1	models					418:423	experimental models	405:423	experimental models	405:423	However, controversy exists regarding to the safety of carrageenan due to its exacerbation of colitis in experimental models.
31926226	7	5	theme	microbiota	1063:1072	arg1	composition					1044:1054	the composition	1040:1054	the composition of gut microbiota	1040:1072	Moreover, we discovered that carrageenan-induced colitis in high-fat diet model was robustly correlated with changes in the composition of gut microbiota, specifically Alistipes finegoldii and Bacteroides acidifaciens.
31926226	8	6	theme	inflammatory	1166:1177	arg1	property					1179:1186	the inflammatory property	1162:1186	the inflammatory property of carrageenan	1162:1201	Hence, we propose that the inflammatory property of carrageenan is influenced greatly by its intake form via modification of host intestinal microecology.
31926226	8	7	theme	microecology	1280:1291	arg1	modification					1248:1259	modification	1248:1259	modification of host intestinal microecology	1248:1291	Hence, we propose that the inflammatory property of carrageenan is influenced greatly by its intake form via modification of host intestinal microecology.
31926226	8	8	theme	carrageenan	1191:1201	arg1	property					1179:1186	the inflammatory property	1162:1186	the inflammatory property of carrageenan	1162:1201	Hence, we propose that the inflammatory property of carrageenan is influenced greatly by its intake form via modification of host intestinal microecology.
31926226	4	9	theme	C57BL/6 J	548:556	arg1	mice					558:561	C57BL/6 J mice	548:561	C57BL/6 J mice	548:561	In this study, we studied the effects of vehicle and host intestinal microflora on carrageenan inflammatory properties in C57BL/6 J mice.
31926226	4	10	theme	vehicle	467:473	arg1	microflora					495:504	vehicle and host intestinal microflora	467:504	vehicle and host intestinal microflora	467:504	In this study, we studied the effects of vehicle and host intestinal microflora on carrageenan inflammatory properties in C57BL/6 J mice.
31926226	5	11	theme	drinking	624:631	arg1	water					633:637	drinking water	624:637	drinking water	624:637	We found that in high-fat diet model, native carrageenan in drinking water increased the disease activity index (DAI), myeloperoxidase (MPO) activity and the mRNA expression of TLR4 in colon, whereas carrageenan-supplemented diet has no visible effects.
31926226	5	12	theme	myeloperoxidase	683:697	arg1	activity					705:712	myeloperoxidase (MPO) activity	683:712	myeloperoxidase (MPO) activity	683:712	We found that in high-fat diet model, native carrageenan in drinking water increased the disease activity index (DAI), myeloperoxidase (MPO) activity and the mRNA expression of TLR4 in colon, whereas carrageenan-supplemented diet has no visible effects.
31926226	4	13	from	effects	456:462	arg1	properties					534:543	carrageenan inflammatory properties	509:543	carrageenan inflammatory properties in C57BL/6 J mice	509:561	In this study, we studied the effects of vehicle and host intestinal microflora on carrageenan inflammatory properties in C57BL/6 J mice.
31926226	5	14	theme	diet	590:593	arg1	model					595:599	high-fat diet model	581:599	high-fat diet model	581:599	We found that in high-fat diet model, native carrageenan in drinking water increased the disease activity index (DAI), myeloperoxidase (MPO) activity and the mRNA expression of TLR4 in colon, whereas carrageenan-supplemented diet has no visible effects.
31926226	5	15	theme	high-fat	581:588	arg1	model					595:599	high-fat diet model	581:599	high-fat diet model	581:599	We found that in high-fat diet model, native carrageenan in drinking water increased the disease activity index (DAI), myeloperoxidase (MPO) activity and the mRNA expression of TLR4 in colon, whereas carrageenan-supplemented diet has no visible effects.
31926226	1	16	theme	Inflammatory	81:92	arg1	IBD					109:111	IBD	109:111	IBD	109:111	Inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis, has gradually emerged as a public health challenge worldwide.
31926226	1	16	theme	Inflammatory	81:92	arg1	disease					100:106	Inflammatory bowel disease	81:106	Inflammatory bowel disease (IBD)	81:112	Inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis, has gradually emerged as a public health challenge worldwide.
31926226	3	17	theme	experimental	405:416	arg1	models					418:423	experimental models	405:423	experimental models	405:423	However, controversy exists regarding to the safety of carrageenan due to its exacerbation of colitis in experimental models.
31926226	5	18	theme	TLR4	741:744	arg1	activity					705:712	myeloperoxidase (MPO) activity	683:712	myeloperoxidase (MPO) activity	683:712	We found that in high-fat diet model, native carrageenan in drinking water increased the disease activity index (DAI), myeloperoxidase (MPO) activity and the mRNA expression of TLR4 in colon, whereas carrageenan-supplemented diet has no visible effects.
31926226	5	18	theme	TLR4	741:744	arg1	DAI					677:679	DAI	677:679	DAI	677:679	We found that in high-fat diet model, native carrageenan in drinking water increased the disease activity index (DAI), myeloperoxidase (MPO) activity and the mRNA expression of TLR4 in colon, whereas carrageenan-supplemented diet has no visible effects.
31926226	5	18	theme	TLR4	741:744	arg1	expression					727:736	the mRNA expression	718:736	the mRNA expression of TLR4	718:744	We found that in high-fat diet model, native carrageenan in drinking water increased the disease activity index (DAI), myeloperoxidase (MPO) activity and the mRNA expression of TLR4 in colon, whereas carrageenan-supplemented diet has no visible effects.
31926226	5	18	theme	TLR4	741:744	arg1	index					670:674	the disease activity index	649:674	the disease activity index (DAI)	649:680	We found that in high-fat diet model, native carrageenan in drinking water increased the disease activity index (DAI), myeloperoxidase (MPO) activity and the mRNA expression of TLR4 in colon, whereas carrageenan-supplemented diet has no visible effects.
31926226	1	19	theme	bowel	94:98	arg1	IBD					109:111	IBD	109:111	IBD	109:111	Inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis, has gradually emerged as a public health challenge worldwide.
31926226	1	19	theme	bowel	94:98	arg1	disease					100:106	Inflammatory bowel disease	81:106	Inflammatory bowel disease (IBD)	81:112	Inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis, has gradually emerged as a public health challenge worldwide.
31926226	0	20	theme	κ-carrageenan	7:19	arg1	induced-colitis					21:35	Native κ-carrageenan induced-colitis	0:35	Native κ-carrageenan induced-colitis	0:35	Native κ-carrageenan induced-colitis is related to host intestinal microecology.
31926226	5	21	contain	has	794:796	arg2	effects					809:815	no visible effects	798:815	no visible effects	798:815	We found that in high-fat diet model, native carrageenan in drinking water increased the disease activity index (DAI), myeloperoxidase (MPO) activity and the mRNA expression of TLR4 in colon, whereas carrageenan-supplemented diet has no visible effects.
31926226	5	21	contain	has	794:796	arg1	diet					789:792	carrageenan-supplemented diet	764:792	carrageenan-supplemented diet	764:792	We found that in high-fat diet model, native carrageenan in drinking water increased the disease activity index (DAI), myeloperoxidase (MPO) activity and the mRNA expression of TLR4 in colon, whereas carrageenan-supplemented diet has no visible effects.
31926226	3	22	theme	colitis	394:400	arg1	exacerbation					378:389	its exacerbation	374:389	its exacerbation of colitis in experimental models	374:423	However, controversy exists regarding to the safety of carrageenan due to its exacerbation of colitis in experimental models.
31926226	0	23	theme	Native	0:5	arg1	induced-colitis					21:35	Native κ-carrageenan induced-colitis	0:35	Native κ-carrageenan induced-colitis	0:35	Native κ-carrageenan induced-colitis is related to host intestinal microecology.
31926226	4	24	theme	inflammatory	521:532	arg1	properties					534:543	carrageenan inflammatory properties	509:543	carrageenan inflammatory properties in C57BL/6 J mice	509:561	In this study, we studied the effects of vehicle and host intestinal microflora on carrageenan inflammatory properties in C57BL/6 J mice.
31926226	5	25	theme	native	602:607	arg1	carrageenan					609:619	native carrageenan	602:619	native carrageenan in drinking water	602:637	We found that in high-fat diet model, native carrageenan in drinking water increased the disease activity index (DAI), myeloperoxidase (MPO) activity and the mRNA expression of TLR4 in colon, whereas carrageenan-supplemented diet has no visible effects.
31926226	4	26	theme	carrageenan	509:519	arg1	properties					534:543	carrageenan inflammatory properties	509:543	carrageenan inflammatory properties in C57BL/6 J mice	509:561	In this study, we studied the effects of vehicle and host intestinal microflora on carrageenan inflammatory properties in C57BL/6 J mice.
31926226	5	27	theme	carrageenan-supplemented	764:787	arg1	diet					789:792	carrageenan-supplemented diet	764:792	carrageenan-supplemented diet	764:792	We found that in high-fat diet model, native carrageenan in drinking water increased the disease activity index (DAI), myeloperoxidase (MPO) activity and the mRNA expression of TLR4 in colon, whereas carrageenan-supplemented diet has no visible effects.
31926226	4	28	from	properties	534:543	arg1	mice					558:561	C57BL/6 J mice	548:561	C57BL/6 J mice	548:561	In this study, we studied the effects of vehicle and host intestinal microflora on carrageenan inflammatory properties in C57BL/6 J mice.
31926226	5	29	theme	mRNA	722:725	arg1	expression					727:736	the mRNA expression	718:736	the mRNA expression of TLR4	718:744	We found that in high-fat diet model, native carrageenan in drinking water increased the disease activity index (DAI), myeloperoxidase (MPO) activity and the mRNA expression of TLR4 in colon, whereas carrageenan-supplemented diet has no visible effects.
31926226	8	30	theme	intake	1232:1237	arg1	form					1239:1242	its intake form	1228:1242	its intake form	1228:1242	Hence, we propose that the inflammatory property of carrageenan is influenced greatly by its intake form via modification of host intestinal microecology.
31926226	1	31	theme	public	192:197	arg1	worldwide					216:224	a public health challenge worldwide	190:224	a public health challenge worldwide	190:224	Inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis, has gradually emerged as a public health challenge worldwide.
31926226	5	32	theme	visible	801:807	arg1	effects					809:815	no visible effects	798:815	no visible effects	798:815	We found that in high-fat diet model, native carrageenan in drinking water increased the disease activity index (DAI), myeloperoxidase (MPO) activity and the mRNA expression of TLR4 in colon, whereas carrageenan-supplemented diet has no visible effects.
31926226	4	33	theme	intestinal	484:493	arg1	microflora					495:504	vehicle and host intestinal microflora	467:504	vehicle and host intestinal microflora	467:504	In this study, we studied the effects of vehicle and host intestinal microflora on carrageenan inflammatory properties in C57BL/6 J mice.
31926226	1	34	theme	health	199:204	arg1	worldwide					216:224	a public health challenge worldwide	190:224	a public health challenge worldwide	190:224	Inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis, has gradually emerged as a public health challenge worldwide.
31926226	0	35	theme	intestinal	56:65	arg1	microecology					67:78	host intestinal microecology	51:78	host intestinal microecology	51:78	Native κ-carrageenan induced-colitis is related to host intestinal microecology.
31926226	4	36	theme	host	479:482	arg1	microflora					495:504	vehicle and host intestinal microflora	467:504	vehicle and host intestinal microflora	467:504	In this study, we studied the effects of vehicle and host intestinal microflora on carrageenan inflammatory properties in C57BL/6 J mice.
31926226	7	37	theme	high-fat	980:987	arg1	model					994:998	high-fat diet model	980:998	high-fat diet model	980:998	Moreover, we discovered that carrageenan-induced colitis in high-fat diet model was robustly correlated with changes in the composition of gut microbiota, specifically Alistipes finegoldii and Bacteroides acidifaciens.
31926226	7	38	from	changes	1029:1035	arg1	composition					1044:1054	the composition	1040:1054	the composition of gut microbiota	1040:1072	Moreover, we discovered that carrageenan-induced colitis in high-fat diet model was robustly correlated with changes in the composition of gut microbiota, specifically Alistipes finegoldii and Bacteroides acidifaciens.
31926226	7	39	theme	carrageenan-induced	949:967	arg1	colitis					969:975	carrageenan-induced colitis	949:975	carrageenan-induced colitis in high-fat diet model	949:998	Moreover, we discovered that carrageenan-induced colitis in high-fat diet model was robustly correlated with changes in the composition of gut microbiota, specifically Alistipes finegoldii and Bacteroides acidifaciens.
31926226	0	40	theme	host	51:54	arg1	microecology					67:78	host intestinal microecology	51:78	host intestinal microecology	51:78	Native κ-carrageenan induced-colitis is related to host intestinal microecology.
31926226	5	41	from	carrageenan	609:619	arg1	water					633:637	drinking water	624:637	drinking water	624:637	We found that in high-fat diet model, native carrageenan in drinking water increased the disease activity index (DAI), myeloperoxidase (MPO) activity and the mRNA expression of TLR4 in colon, whereas carrageenan-supplemented diet has no visible effects.
31926226	7	42	theme	diet	989:992	arg1	model					994:998	high-fat diet model	980:998	high-fat diet model	980:998	Moreover, we discovered that carrageenan-induced colitis in high-fat diet model was robustly correlated with changes in the composition of gut microbiota, specifically Alistipes finegoldii and Bacteroides acidifaciens.
31926226	4	43	theme	microflora	495:504	arg1	effects					456:462	the effects	452:462	the effects of vehicle and host intestinal microflora on carrageenan inflammatory properties in C57BL/6 J mice	452:561	In this study, we studied the effects of vehicle and host intestinal microflora on carrageenan inflammatory properties in C57BL/6 J mice.
31926226	5	44	theme	disease	653:659	arg1	DAI					677:679	DAI	677:679	DAI	677:679	We found that in high-fat diet model, native carrageenan in drinking water increased the disease activity index (DAI), myeloperoxidase (MPO) activity and the mRNA expression of TLR4 in colon, whereas carrageenan-supplemented diet has no visible effects.
31926226	5	44	theme	disease	653:659	arg1	index					670:674	the disease activity index	649:674	the disease activity index (DAI)	649:680	We found that in high-fat diet model, native carrageenan in drinking water increased the disease activity index (DAI), myeloperoxidase (MPO) activity and the mRNA expression of TLR4 in colon, whereas carrageenan-supplemented diet has no visible effects.
31926226	1	45	theme	challenge	206:214	arg1	worldwide					216:224	a public health challenge worldwide	190:224	a public health challenge worldwide	190:224	Inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis, has gradually emerged as a public health challenge worldwide.
31926226	2	46	theme	additive	257:264	arg1	Carrageenan					227:237	Carrageenan	227:237	Carrageenan	227:237	Carrageenan is a popular food additive that has been in use for decades.
31926226	2	46	theme	additive	257:264	arg1	food					252:255	a popular food	242:255	a popular food additive that has been in use for decades	242:297	Carrageenan is a popular food additive that has been in use for decades.
31926226	2	47	theme	popular	244:250	arg1	Carrageenan					227:237	Carrageenan	227:237	Carrageenan	227:237	Carrageenan is a popular food additive that has been in use for decades.
31926226	2	47	theme	popular	244:250	arg1	food					252:255	a popular food	242:255	a popular food additive that has been in use for decades	242:297	Carrageenan is a popular food additive that has been in use for decades.
31926226	8	48	theme	host	1264:1267	arg1	microecology					1280:1291	host intestinal microecology	1264:1291	host intestinal microecology	1264:1291	Hence, we propose that the inflammatory property of carrageenan is influenced greatly by its intake form via modification of host intestinal microecology.
31926226	7	49	from	colitis	969:975	arg1	model					994:998	high-fat diet model	980:998	high-fat diet model	980:998	Moreover, we discovered that carrageenan-induced colitis in high-fat diet model was robustly correlated with changes in the composition of gut microbiota, specifically Alistipes finegoldii and Bacteroides acidifaciens.
31926226	8	50	theme	intestinal	1269:1278	arg1	microecology					1280:1291	host intestinal microecology	1264:1291	host intestinal microecology	1264:1291	Hence, we propose that the inflammatory property of carrageenan is influenced greatly by its intake form via modification of host intestinal microecology.
31926226	6	51	theme	colitis	839:845	arg1	signs					830:834	no signs	827:834	no signs of colitis	827:845	However, no signs of colitis were observed under low-fat diet regardless of the mode of vehicle used.
31926226	6	52	theme	vehicle	906:912	arg1	mode					898:901	the mode	894:901	the mode of vehicle used	894:917	However, no signs of colitis were observed under low-fat diet regardless of the mode of vehicle used.
31926226	3	53	theme	carrageenan	355:365	arg1	safety					345:350	the safety	341:350	the safety of carrageenan due to its exacerbation of colitis in experimental models	341:423	However, controversy exists regarding to the safety of carrageenan due to its exacerbation of colitis in experimental models.
32607725	1	0	theme	genus	129:133	arg1	Parvularcula					135:146	the genus Parvularcula	125:146	the genus Parvularcula	125:146	To date, the genus Parvularcula consists of 6 species and no potential application of this genus was reported.
32607725	8	1	used	used	1305:1308	arg2	strain					1289:1294	the strain	1285:1294	the strain	1285:1294	These findings suggest the potential of Parvularcula flava strain NH6-79 T in cellulose-containing biomass degradation and that the strain could be used in cellulosic biorefining process.
32607725	8	2	from	potential	1184:1192	arg1	degradation					1264:1274	cellulose-containing biomass degradation	1235:1274	cellulose-containing biomass degradation	1235:1274	These findings suggest the potential of Parvularcula flava strain NH6-79 T in cellulose-containing biomass degradation and that the strain could be used in cellulosic biorefining process.
32607725	2	3	theme	enzyme	329:334	arg1	analysis					336:343	its cellulolytic enzyme analysis	312:343	its cellulolytic enzyme analysis	312:343	Current study presents the genome sequence of Parvularcula flava strain NH6-79 T and its cellulolytic enzyme analysis.
32607725	5	4	theme	biomass	634:640	arg1	degradation					642:652	cellulosic biomass degradation	623:652	cellulosic biomass degradation	623:652	Within these GHs, 20 encoded genes are related to cellulosic biomass degradation, including 12 endoglucanases (5 GH10, 4 GH5, and 3 GH51), 2 exoglucanases (GH9) and 6 β-glucosidases (GH3).
32607725	7	5	with	combination	986:996	arg1	studies					1035:1041	experimental studies	1022:1041	experimental studies	1022:1041	The combination of genome analysis with experimental studies indicated the ability of strain NH6-79 T to produce extracellular endoglucanase, exoglucanase, and β-glucosidase.
32607725	4	6	theme	them	523:526	arg1	hydrolases					555:564	glycoside hydrolases	545:564	glycoside hydrolases (GHs)	545:570	From a total of 3,465 genes predicted, 96 of them are annotated as glycoside hydrolases (GHs).
32607725	4	6	theme	them	523:526	arg1	them					523:526	them	523:526	them	523:526	From a total of 3,465 genes predicted, 96 of them are annotated as glycoside hydrolases (GHs).
32607725	4	6	theme	them	523:526	arg1	96					517:518	96	517:518	96	517:518	From a total of 3,465 genes predicted, 96 of them are annotated as glycoside hydrolases (GHs).
32607725	6	7	located	observed	864:871	arg1	hour					881:884	27th hour	876:884	27th hour	876:884	In addition, highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase) were observed at 27th hour when the strain was cultured in the carboxymethyl cellulose/Avicel®-containing medium for 45 h.
32607725	6	7	located	observed	864:871	arg1	addition					765:772	addition	765:772	addition	765:772	In addition, highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase) were observed at 27th hour when the strain was cultured in the carboxymethyl cellulose/Avicel®-containing medium for 45 h.
32607725	6	7	located	observed	864:871	arg2	exoglucanase					826:837	exoglucanase	826:837	exoglucanase	826:837	In addition, highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase) were observed at 27th hour when the strain was cultured in the carboxymethyl cellulose/Avicel®-containing medium for 45 h.
32607725	6	7	located	observed	864:871	arg2	β-glucosidase					844:856	β-glucosidase	844:856	β-glucosidase	844:856	In addition, highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase) were observed at 27th hour when the strain was cultured in the carboxymethyl cellulose/Avicel®-containing medium for 45 h.
32607725	6	7	located	observed	864:871	arg2	activities					799:808	highest relative enzyme activities	775:808	highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase)	775:857	In addition, highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase) were observed at 27th hour when the strain was cultured in the carboxymethyl cellulose/Avicel®-containing medium for 45 h.
32607725	6	7	located	observed	864:871	arg2	endoglucanase					811:823	endoglucanase	811:823	endoglucanase	811:823	In addition, highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase) were observed at 27th hour when the strain was cultured in the carboxymethyl cellulose/Avicel®-containing medium for 45 h.
32607725	2	8	theme	genome	254:259	arg1	sequence					261:268	the genome sequence	250:268	the genome sequence of Parvularcula flava strain NH6-79 T	250:306	Current study presents the genome sequence of Parvularcula flava strain NH6-79 T and its cellulolytic enzyme analysis.
32607725	0	9	theme	enzymes	98:104	arg1	producer					106:113	a cellulolytic enzymes producer	83:113	a cellulolytic enzymes producer	83:113	Draft genome sequence of Parvularcula flava strain NH6-79 T, revealing its role as a cellulolytic enzymes producer.
32607725	6	10	theme	carboxymethyl	922:934	arg1	medium					965:970	the carboxymethyl cellulose/Avicel®-containing medium	918:970	the carboxymethyl cellulose/Avicel®-containing medium	918:970	In addition, highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase) were observed at 27th hour when the strain was cultured in the carboxymethyl cellulose/Avicel®-containing medium for 45 h.
32607725	8	11	theme	biomass	1256:1262	arg1	degradation					1264:1274	cellulose-containing biomass degradation	1235:1274	cellulose-containing biomass degradation	1235:1274	These findings suggest the potential of Parvularcula flava strain NH6-79 T in cellulose-containing biomass degradation and that the strain could be used in cellulosic biorefining process.
32607725	8	12	theme	strain	1216:1221	arg1	potential					1184:1192	the potential	1180:1192	the potential of Parvularcula flava strain NH6-79 T in cellulose-containing biomass degradation	1180:1274	These findings suggest the potential of Parvularcula flava strain NH6-79 T in cellulose-containing biomass degradation and that the strain could be used in cellulosic biorefining process.
32607725	0	13	theme	cellulolytic	85:96	arg1	producer					106:113	a cellulolytic enzymes producer	83:113	a cellulolytic enzymes producer	83:113	Draft genome sequence of Parvularcula flava strain NH6-79 T, revealing its role as a cellulolytic enzymes producer.
32607725	7	14	theme	genome	1001:1006	arg1	analysis					1008:1015	genome analysis	1001:1015	genome analysis	1001:1015	The combination of genome analysis with experimental studies indicated the ability of strain NH6-79 T to produce extracellular endoglucanase, exoglucanase, and β-glucosidase.
32607725	8	15	theme	cellulose-containing	1235:1254	arg1	degradation					1264:1274	cellulose-containing biomass degradation	1235:1274	cellulose-containing biomass degradation	1235:1274	These findings suggest the potential of Parvularcula flava strain NH6-79 T in cellulose-containing biomass degradation and that the strain could be used in cellulosic biorefining process.
32607725	0	16	theme	genome	6:11	arg1	sequence					13:20	Draft genome sequence	0:20	Draft genome sequence of Parvularcula flava strain	0:49	Draft genome sequence of Parvularcula flava strain NH6-79 T, revealing its role as a cellulolytic enzymes producer.
32607725	2	17	theme	strain	292:297	arg1	NH6-79 T					299:306	Parvularcula flava strain NH6-79 T	273:306	Parvularcula flava strain NH6-79 T	273:306	Current study presents the genome sequence of Parvularcula flava strain NH6-79 T and its cellulolytic enzyme analysis.
32607725	0	18	theme	Draft	0:4	arg1	sequence					13:20	Draft genome sequence	0:20	Draft genome sequence of Parvularcula flava strain	0:49	Draft genome sequence of Parvularcula flava strain NH6-79 T, revealing its role as a cellulolytic enzymes producer.
32607725	4	19	theme	3,465	494:498	arg1	genes					500:504	3,465 genes	494:504	3,465 genes predicted	494:514	From a total of 3,465 genes predicted, 96 of them are annotated as glycoside hydrolases (GHs).
32607725	2	20	theme	flava	286:290	arg1	NH6-79 T					299:306	Parvularcula flava strain NH6-79 T	273:306	Parvularcula flava strain NH6-79 T	273:306	Current study presents the genome sequence of Parvularcula flava strain NH6-79 T and its cellulolytic enzyme analysis.
32607725	0	21	theme	strain	44:49	arg1	sequence					13:20	Draft genome sequence	0:20	Draft genome sequence of Parvularcula flava strain	0:49	Draft genome sequence of Parvularcula flava strain NH6-79 T, revealing its role as a cellulolytic enzymes producer.
32607725	3	22	theme	assembled	350:358	arg1	genome					366:371	The assembled draft genome	346:371	The assembled draft genome of strain NH6-79 T	346:390	The assembled draft genome of strain NH6-79 T consists of 9 contigs and 7 scaffolds with 3.68 Mbp in size and GC content of 59.87%.
32607725	3	23	with	scaffolds	420:428	arg1	Mbp					440:442	3.68 Mbp	435:442	3.68 Mbp	435:442	The assembled draft genome of strain NH6-79 T consists of 9 contigs and 7 scaffolds with 3.68 Mbp in size and GC content of 59.87%.
32607725	2	24	theme	Parvularcula	273:284	arg1	NH6-79 T					299:306	Parvularcula flava strain NH6-79 T	273:306	Parvularcula flava strain NH6-79 T	273:306	Current study presents the genome sequence of Parvularcula flava strain NH6-79 T and its cellulolytic enzyme analysis.
32607725	3	25	theme	draft	360:364	arg1	genome					366:371	The assembled draft genome	346:371	The assembled draft genome of strain NH6-79 T	346:390	The assembled draft genome of strain NH6-79 T consists of 9 contigs and 7 scaffolds with 3.68 Mbp in size and GC content of 59.87%.
32607725	8	26	theme	flava	1210:1214	arg1	strain					1216:1221	Parvularcula flava strain NH6-79 T	1197:1230	Parvularcula flava strain NH6-79 T	1197:1230	These findings suggest the potential of Parvularcula flava strain NH6-79 T in cellulose-containing biomass degradation and that the strain could be used in cellulosic biorefining process.
32607725	7	27	theme	analysis	1008:1015	arg1	combination					986:996	The combination	982:996	The combination of genome analysis with experimental studies	982:1041	The combination of genome analysis with experimental studies indicated the ability of strain NH6-79 T to produce extracellular endoglucanase, exoglucanase, and β-glucosidase.
32607725	0	28	theme	flava	38:42	arg1	strain					44:49	Parvularcula flava strain	25:49	Parvularcula flava strain	25:49	Draft genome sequence of Parvularcula flava strain NH6-79 T, revealing its role as a cellulolytic enzymes producer.
32607725	4	29	theme	genes	500:504	arg1	total					485:489	a total	483:489	a total of 3,465 genes predicted	483:514	From a total of 3,465 genes predicted, 96 of them are annotated as glycoside hydrolases (GHs).
32607725	1	30	theme	potential	177:185	arg1	application					187:197	no potential application	174:197	no potential application of this genus	174:211	To date, the genus Parvularcula consists of 6 species and no potential application of this genus was reported.
32607725	0	31	theme	Parvularcula	25:36	arg1	strain					44:49	Parvularcula flava strain	25:49	Parvularcula flava strain	25:49	Draft genome sequence of Parvularcula flava strain NH6-79 T, revealing its role as a cellulolytic enzymes producer.
32607725	6	32	theme	enzyme	792:797	arg1	exoglucanase					826:837	exoglucanase	826:837	exoglucanase	826:837	In addition, highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase) were observed at 27th hour when the strain was cultured in the carboxymethyl cellulose/Avicel®-containing medium for 45 h.
32607725	6	32	theme	enzyme	792:797	arg1	β-glucosidase					844:856	β-glucosidase	844:856	β-glucosidase	844:856	In addition, highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase) were observed at 27th hour when the strain was cultured in the carboxymethyl cellulose/Avicel®-containing medium for 45 h.
32607725	6	32	theme	enzyme	792:797	arg1	endoglucanase					811:823	endoglucanase	811:823	endoglucanase	811:823	In addition, highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase) were observed at 27th hour when the strain was cultured in the carboxymethyl cellulose/Avicel®-containing medium for 45 h.
32607725	6	32	theme	enzyme	792:797	arg1	activities					799:808	highest relative enzyme activities	775:808	highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase)	775:857	In addition, highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase) were observed at 27th hour when the strain was cultured in the carboxymethyl cellulose/Avicel®-containing medium for 45 h.
32607725	5	33	theme	cellulosic	623:632	arg1	degradation					642:652	cellulosic biomass degradation	623:652	cellulosic biomass degradation	623:652	Within these GHs, 20 encoded genes are related to cellulosic biomass degradation, including 12 endoglucanases (5 GH10, 4 GH5, and 3 GH51), 2 exoglucanases (GH9) and 6 β-glucosidases (GH3).
32607725	4	34	theme	glycoside	545:553	arg1	GHs					567:569	GHs	567:569	GHs	567:569	From a total of 3,465 genes predicted, 96 of them are annotated as glycoside hydrolases (GHs).
32607725	4	34	theme	glycoside	545:553	arg1	hydrolases					555:564	glycoside hydrolases	545:564	glycoside hydrolases (GHs)	545:570	From a total of 3,465 genes predicted, 96 of them are annotated as glycoside hydrolases (GHs).
32607725	4	34	theme	glycoside	545:553	arg1	them					523:526	them	523:526	them	523:526	From a total of 3,465 genes predicted, 96 of them are annotated as glycoside hydrolases (GHs).
32607725	4	34	theme	glycoside	545:553	arg1	96					517:518	96	517:518	96	517:518	From a total of 3,465 genes predicted, 96 of them are annotated as glycoside hydrolases (GHs).
32607725	3	35	from	scaffolds	420:428	arg1	size					447:450	size	447:450	size	447:450	The assembled draft genome of strain NH6-79 T consists of 9 contigs and 7 scaffolds with 3.68 Mbp in size and GC content of 59.87%.
32607725	3	35	from	scaffolds	420:428	arg1	content					459:465	GC content	456:465	GC content	456:465	The assembled draft genome of strain NH6-79 T consists of 9 contigs and 7 scaffolds with 3.68 Mbp in size and GC content of 59.87%.
32607725	6	36	theme	27th	876:879	arg1	hour					881:884	27th hour	876:884	27th hour	876:884	In addition, highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase) were observed at 27th hour when the strain was cultured in the carboxymethyl cellulose/Avicel®-containing medium for 45 h.
32607725	3	37	theme	strain	376:381	arg1	NH6-79 T					383:390	strain NH6-79 T	376:390	strain NH6-79 T	376:390	The assembled draft genome of strain NH6-79 T consists of 9 contigs and 7 scaffolds with 3.68 Mbp in size and GC content of 59.87%.
32607725	6	38	theme	relative	783:790	arg1	exoglucanase					826:837	exoglucanase	826:837	exoglucanase	826:837	In addition, highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase) were observed at 27th hour when the strain was cultured in the carboxymethyl cellulose/Avicel®-containing medium for 45 h.
32607725	6	38	theme	relative	783:790	arg1	β-glucosidase					844:856	β-glucosidase	844:856	β-glucosidase	844:856	In addition, highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase) were observed at 27th hour when the strain was cultured in the carboxymethyl cellulose/Avicel®-containing medium for 45 h.
32607725	6	38	theme	relative	783:790	arg1	endoglucanase					811:823	endoglucanase	811:823	endoglucanase	811:823	In addition, highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase) were observed at 27th hour when the strain was cultured in the carboxymethyl cellulose/Avicel®-containing medium for 45 h.
32607725	6	38	theme	relative	783:790	arg1	activities					799:808	highest relative enzyme activities	775:808	highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase)	775:857	In addition, highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase) were observed at 27th hour when the strain was cultured in the carboxymethyl cellulose/Avicel®-containing medium for 45 h.
32607725	2	39	theme	cellulolytic	316:327	arg1	analysis					336:343	its cellulolytic enzyme analysis	312:343	its cellulolytic enzyme analysis	312:343	Current study presents the genome sequence of Parvularcula flava strain NH6-79 T and its cellulolytic enzyme analysis.
32607725	3	40	theme	NH6-79 T	383:390	arg1	genome					366:371	The assembled draft genome	346:371	The assembled draft genome of strain NH6-79 T	346:390	The assembled draft genome of strain NH6-79 T consists of 9 contigs and 7 scaffolds with 3.68 Mbp in size and GC content of 59.87%.
32607725	6	41	theme	highest	775:781	arg1	exoglucanase					826:837	exoglucanase	826:837	exoglucanase	826:837	In addition, highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase) were observed at 27th hour when the strain was cultured in the carboxymethyl cellulose/Avicel®-containing medium for 45 h.
32607725	6	41	theme	highest	775:781	arg1	β-glucosidase					844:856	β-glucosidase	844:856	β-glucosidase	844:856	In addition, highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase) were observed at 27th hour when the strain was cultured in the carboxymethyl cellulose/Avicel®-containing medium for 45 h.
32607725	6	41	theme	highest	775:781	arg1	endoglucanase					811:823	endoglucanase	811:823	endoglucanase	811:823	In addition, highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase) were observed at 27th hour when the strain was cultured in the carboxymethyl cellulose/Avicel®-containing medium for 45 h.
32607725	6	41	theme	highest	775:781	arg1	activities					799:808	highest relative enzyme activities	775:808	highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase)	775:857	In addition, highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase) were observed at 27th hour when the strain was cultured in the carboxymethyl cellulose/Avicel®-containing medium for 45 h.
32607725	3	42	with	contigs	406:412	arg1	Mbp					440:442	3.68 Mbp	435:442	3.68 Mbp	435:442	The assembled draft genome of strain NH6-79 T consists of 9 contigs and 7 scaffolds with 3.68 Mbp in size and GC content of 59.87%.
32607725	3	43	theme	GC	456:457	arg1	content					459:465	GC content	456:465	GC content	456:465	The assembled draft genome of strain NH6-79 T consists of 9 contigs and 7 scaffolds with 3.68 Mbp in size and GC content of 59.87%.
32607725	5	44	dep	endoglucanases	668:681	arg1	endoglucanases					668:681	12 endoglucanases	665:681	12 endoglucanases (5 GH10, 4 GH5, and 3 GH51)	665:709	Within these GHs, 20 encoded genes are related to cellulosic biomass degradation, including 12 endoglucanases (5 GH10, 4 GH5, and 3 GH51), 2 exoglucanases (GH9) and 6 β-glucosidases (GH3).
32607725	5	44	dep	endoglucanases	668:681	arg1	GH10					686:689	5 GH10	684:689	5 GH10	684:689	Within these GHs, 20 encoded genes are related to cellulosic biomass degradation, including 12 endoglucanases (5 GH10, 4 GH5, and 3 GH51), 2 exoglucanases (GH9) and 6 β-glucosidases (GH3).
32607725	5	44	dep	endoglucanases	668:681	arg1	GH5					694:696	4 GH5	692:696	4 GH5	692:696	Within these GHs, 20 encoded genes are related to cellulosic biomass degradation, including 12 endoglucanases (5 GH10, 4 GH5, and 3 GH51), 2 exoglucanases (GH9) and 6 β-glucosidases (GH3).
32607725	5	44	dep	endoglucanases	668:681	arg1	GH51					705:708	3 GH51	703:708	3 GH51	703:708	Within these GHs, 20 encoded genes are related to cellulosic biomass degradation, including 12 endoglucanases (5 GH10, 4 GH5, and 3 GH51), 2 exoglucanases (GH9) and 6 β-glucosidases (GH3).
32607725	8	45	theme	biorefining	1324:1334	arg1	process					1336:1342	cellulosic biorefining process	1313:1342	cellulosic biorefining process	1313:1342	These findings suggest the potential of Parvularcula flava strain NH6-79 T in cellulose-containing biomass degradation and that the strain could be used in cellulosic biorefining process.
32607725	5	46	theme	encoded	594:600	arg1	genes					602:606	20 encoded genes	591:606	20 encoded genes	591:606	Within these GHs, 20 encoded genes are related to cellulosic biomass degradation, including 12 endoglucanases (5 GH10, 4 GH5, and 3 GH51), 2 exoglucanases (GH9) and 6 β-glucosidases (GH3).
32607725	6	47	dep	activities	799:808	arg1	exoglucanase					826:837	exoglucanase	826:837	exoglucanase	826:837	In addition, highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase) were observed at 27th hour when the strain was cultured in the carboxymethyl cellulose/Avicel®-containing medium for 45 h.
32607725	6	47	dep	activities	799:808	arg1	β-glucosidase					844:856	β-glucosidase	844:856	β-glucosidase	844:856	In addition, highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase) were observed at 27th hour when the strain was cultured in the carboxymethyl cellulose/Avicel®-containing medium for 45 h.
32607725	6	47	dep	activities	799:808	arg1	endoglucanase					811:823	endoglucanase	811:823	endoglucanase	811:823	In addition, highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase) were observed at 27th hour when the strain was cultured in the carboxymethyl cellulose/Avicel®-containing medium for 45 h.
32607725	6	47	dep	activities	799:808	arg1	activities					799:808	highest relative enzyme activities	775:808	highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase)	775:857	In addition, highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase) were observed at 27th hour when the strain was cultured in the carboxymethyl cellulose/Avicel®-containing medium for 45 h.
32607725	8	48	theme	cellulosic	1313:1322	arg1	process					1336:1342	cellulosic biorefining process	1313:1342	cellulosic biorefining process	1313:1342	These findings suggest the potential of Parvularcula flava strain NH6-79 T in cellulose-containing biomass degradation and that the strain could be used in cellulosic biorefining process.
32607725	2	49	theme	Current	227:233	arg1	study					235:239	Current study	227:239	Current study	227:239	Current study presents the genome sequence of Parvularcula flava strain NH6-79 T and its cellulolytic enzyme analysis.
32607725	2	50	theme	NH6-79 T	299:306	arg1	analysis					336:343	its cellulolytic enzyme analysis	312:343	its cellulolytic enzyme analysis	312:343	Current study presents the genome sequence of Parvularcula flava strain NH6-79 T and its cellulolytic enzyme analysis.
32607725	2	50	theme	NH6-79 T	299:306	arg1	sequence					261:268	the genome sequence	250:268	the genome sequence of Parvularcula flava strain NH6-79 T	250:306	Current study presents the genome sequence of Parvularcula flava strain NH6-79 T and its cellulolytic enzyme analysis.
32607725	6	51	theme	cellulose/Avicel®-containing	936:963	arg1	medium					965:970	the carboxymethyl cellulose/Avicel®-containing medium	918:970	the carboxymethyl cellulose/Avicel®-containing medium	918:970	In addition, highest relative enzyme activities (endoglucanase, exoglucanase, and β-glucosidase) were observed at 27th hour when the strain was cultured in the carboxymethyl cellulose/Avicel®-containing medium for 45 h.
32607725	3	52	from	contigs	406:412	arg1	size					447:450	size	447:450	size	447:450	The assembled draft genome of strain NH6-79 T consists of 9 contigs and 7 scaffolds with 3.68 Mbp in size and GC content of 59.87%.
32607725	3	52	from	contigs	406:412	arg1	content					459:465	GC content	456:465	GC content	456:465	The assembled draft genome of strain NH6-79 T consists of 9 contigs and 7 scaffolds with 3.68 Mbp in size and GC content of 59.87%.
32607725	8	53	theme	Parvularcula	1197:1208	arg1	strain					1216:1221	Parvularcula flava strain NH6-79 T	1197:1230	Parvularcula flava strain NH6-79 T	1197:1230	These findings suggest the potential of Parvularcula flava strain NH6-79 T in cellulose-containing biomass degradation and that the strain could be used in cellulosic biorefining process.
32607725	1	54	theme	genus	207:211	arg1	application					187:197	no potential application	174:197	no potential application of this genus	174:211	To date, the genus Parvularcula consists of 6 species and no potential application of this genus was reported.
32607725	7	55	theme	extracellular	1095:1107	arg1	endoglucanase					1109:1121	extracellular endoglucanase	1095:1121	extracellular endoglucanase	1095:1121	The combination of genome analysis with experimental studies indicated the ability of strain NH6-79 T to produce extracellular endoglucanase, exoglucanase, and β-glucosidase.
32607725	7	56	theme	experimental	1022:1033	arg1	studies					1035:1041	experimental studies	1022:1041	experimental studies	1022:1041	The combination of genome analysis with experimental studies indicated the ability of strain NH6-79 T to produce extracellular endoglucanase, exoglucanase, and β-glucosidase.
32607725	3	57	theme	%	475:475	arg1	size					447:450	size	447:450	size	447:450	The assembled draft genome of strain NH6-79 T consists of 9 contigs and 7 scaffolds with 3.68 Mbp in size and GC content of 59.87%.
32607725	3	57	theme	%	475:475	arg1	content					459:465	GC content	456:465	GC content	456:465	The assembled draft genome of strain NH6-79 T consists of 9 contigs and 7 scaffolds with 3.68 Mbp in size and GC content of 59.87%.
32607725	7	58	theme	strain	1068:1073	arg1	ability					1057:1063	the ability	1053:1063	the ability of strain NH6-79 T to produce extracellular endoglucanase, exoglucanase, and β-glucosidase	1053:1154	The combination of genome analysis with experimental studies indicated the ability of strain NH6-79 T to produce extracellular endoglucanase, exoglucanase, and β-glucosidase.
32065575	10	0	theme	type	1327:1330	arg1	HF60T					1342:1346	HF60T	1342:1346	HF60T (=MCCC 1H00336T=KCTC 33987T)	1342:1375	nov. is proposed, The type strain is HF60T (=MCCC 1H00336T=KCTC 33987T).
32065575	10	0	theme	type	1327:1330	arg1	strain					1332:1337	The type strain	1323:1337	The type strain	1323:1337	nov. is proposed, The type strain is HF60T (=MCCC 1H00336T=KCTC 33987T).
32065575	8	1	theme	rRNA	864:867	arg1	similarity					883:892	97.2 % 16S rRNA gene sequence similarity	853:892	97.2 % 16S rRNA gene sequence similarity	853:892	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	8	1	theme	rRNA	864:867	arg1	13490T					845:850	Exiguobacterium sibiricum JCM 13490T	815:850	Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity)	815:893	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	6	2	dep	anteiso-C13 	548:559	arg1	 0					561:562	 0	561:562	 0	561:562	The major fatty acids were anteiso-C13 : 0, iso-C15 : 0, C16 : 0 and iso-C13 : 0.
32065575	7	3	theme	peptidoglycan	628:640	arg1	l-Lys-Gly					651:659	cell wall peptidoglycan type A3α l-Lys-Gly	618:659	cell wall peptidoglycan type A3α l-Lys-Gly	618:659	The strain had cell wall peptidoglycan type A3α l-Lys-Gly.
32065575	8	4	theme	NBRC	1031:1034	arg1	14763T					1036:1041	Exiguobacterium aurantiacum NBRC 14763T	1003:1041	Exiguobacterium aurantiacum NBRC 14763T (94.5 %)	1003:1050	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	8	4	theme	NBRC	1031:1034	arg1	%					1049:1049	94.5 %	1044:1049	94.5 %	1044:1049	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	8	5	theme	DSM	930:932	arg1	14481T					934:939	Exiguobacterium undae DSM 14481T	908:939	Exiguobacterium undae DSM 14481T (97.1 %)	908:948	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	8	5	theme	DSM	930:932	arg1	%					947:947	97.1 %	942:947	97.1 %	942:947	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	9	6	theme	genetic	1128:1134	arg1	distinctiveness					1136:1150	the phylogenetic and genetic distinctiveness	1107:1150	the phylogenetic and genetic distinctiveness	1107:1150	The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness indicated that strain HF60T can be considered to represent a novel species of the genus Exiguobacterium, for which the name Exiguobacterium flavidum sp.
32065575	7	7	theme	type	642:645	arg1	l-Lys-Gly					651:659	cell wall peptidoglycan type A3α l-Lys-Gly	618:659	cell wall peptidoglycan type A3α l-Lys-Gly	618:659	The strain had cell wall peptidoglycan type A3α l-Lys-Gly.
32065575	2	8	theme	motile	92:97	arg1	strain					163:168	A Gram-stain-positive, motile, facultatively anaerobic, non-sporing, and rod-shaped bacterial strain	69:168	A Gram-stain-positive, motile, facultatively anaerobic, non-sporing, and rod-shaped bacterial strain	69:168	A Gram-stain-positive, motile, facultatively anaerobic, non-sporing, and rod-shaped bacterial strain, designated HF60T, was isolated from the Red Maple Lake of Guizhou Province, China.
32065575	8	9	theme	sequence	874:881	arg1	similarity					883:892	97.2 % 16S rRNA gene sequence similarity	853:892	97.2 % 16S rRNA gene sequence similarity	853:892	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	8	9	theme	sequence	874:881	arg1	13490T					845:850	Exiguobacterium sibiricum JCM 13490T	815:850	Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity)	815:893	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	8	10	theme	genus	761:765	arg1	Exiguobacterium					767:781	the genus Exiguobacterium	757:781	the genus Exiguobacterium	757:781	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	2	11	attach	isolated	193:200	arg1	Lake					221:224	the Red Maple Lake	207:224	the Red Maple Lake of Guizhou Province, China	207:251	A Gram-stain-positive, motile, facultatively anaerobic, non-sporing, and rod-shaped bacterial strain, designated HF60T, was isolated from the Red Maple Lake of Guizhou Province, China.
32065575	2	11	attach	isolated	193:200	arg2	strain					163:168	A Gram-stain-positive, motile, facultatively anaerobic, non-sporing, and rod-shaped bacterial strain	69:168	A Gram-stain-positive, motile, facultatively anaerobic, non-sporing, and rod-shaped bacterial strain	69:168	A Gram-stain-positive, motile, facultatively anaerobic, non-sporing, and rod-shaped bacterial strain, designated HF60T, was isolated from the Red Maple Lake of Guizhou Province, China.
32065575	2	12	theme	China	247:251	arg1	Lake					221:224	the Red Maple Lake	207:224	the Red Maple Lake of Guizhou Province, China	207:251	A Gram-stain-positive, motile, facultatively anaerobic, non-sporing, and rod-shaped bacterial strain, designated HF60T, was isolated from the Red Maple Lake of Guizhou Province, China.
32065575	9	13	theme	differential	1057:1068	arg1	properties					1081:1090	The differential phenotypic properties	1053:1090	The differential phenotypic properties	1053:1090	The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness indicated that strain HF60T can be considered to represent a novel species of the genus Exiguobacterium, for which the name Exiguobacterium flavidum sp.
32065575	1	14	theme	Red	53:55	arg1	Lake					63:66	the Red Maple Lake	49:66	the Red Maple Lake	49:66	nov., isolated from the Red Maple Lake.
32065575	5	15	theme	major	398:402	arg1	phosphatidylethanolamine					422:445	phosphatidylethanolamine	422:445	phosphatidylethanolamine	422:445	The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and aminophosphoglycolipid.
32065575	5	15	theme	major	398:402	arg1	lipids					410:415	The major polar lipids	394:415	The major polar lipids	394:415	The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and aminophosphoglycolipid.
32065575	7	16	theme	wall	623:626	arg1	l-Lys-Gly					651:659	cell wall peptidoglycan type A3α l-Lys-Gly	618:659	cell wall peptidoglycan type A3α l-Lys-Gly	618:659	The strain had cell wall peptidoglycan type A3α l-Lys-Gly.
32065575	9	17	theme	Exiguobacterium	1276:1290	arg1	sp					1301:1302	the name Exiguobacterium flavidum sp	1267:1302	the name Exiguobacterium flavidum sp	1267:1302	The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness indicated that strain HF60T can be considered to represent a novel species of the genus Exiguobacterium, for which the name Exiguobacterium flavidum sp.
32065575	8	18	theme	Exiguobacterium	1003:1017	arg1	14763T					1036:1041	Exiguobacterium aurantiacum NBRC 14763T	1003:1041	Exiguobacterium aurantiacum NBRC 14763T (94.5 %)	1003:1050	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	8	18	theme	Exiguobacterium	1003:1017	arg1	%					1049:1049	94.5 %	1044:1049	94.5 %	1044:1049	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	1	19	theme	Maple	57:61	arg1	Lake					63:66	the Red Maple Lake	49:66	the Red Maple Lake	49:66	nov., isolated from the Red Maple Lake.
32065575	5	20	theme	polar	404:408	arg1	phosphatidylethanolamine					422:445	phosphatidylethanolamine	422:445	phosphatidylethanolamine	422:445	The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and aminophosphoglycolipid.
32065575	5	20	theme	polar	404:408	arg1	lipids					410:415	The major polar lipids	394:415	The major polar lipids	394:415	The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and aminophosphoglycolipid.
32065575	2	21	theme	non-sporing	125:135	arg1	strain					163:168	A Gram-stain-positive, motile, facultatively anaerobic, non-sporing, and rod-shaped bacterial strain	69:168	A Gram-stain-positive, motile, facultatively anaerobic, non-sporing, and rod-shaped bacterial strain	69:168	A Gram-stain-positive, motile, facultatively anaerobic, non-sporing, and rod-shaped bacterial strain, designated HF60T, was isolated from the Red Maple Lake of Guizhou Province, China.
32065575	8	22	theme	gene	702:705	arg1	sequences					707:715	16S rRNA gene sequences	693:715	16S rRNA gene sequences	693:715	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	4	23	theme	predominant	310:320	arg1	MK-8					379:382	MK-8	379:382	MK-8	379:382	The predominant isoprenoid quinones were identified as MK-7 (56.4 %) and MK-8 (35.7 %).
32065575	4	23	theme	predominant	310:320	arg1	quinones					333:340	The predominant isoprenoid quinones	306:340	The predominant isoprenoid quinones	306:340	The predominant isoprenoid quinones were identified as MK-7 (56.4 %) and MK-8 (35.7 %).
32065575	4	23	theme	predominant	310:320	arg1	MK-7					361:364	MK-7	361:364	MK-7	361:364	The predominant isoprenoid quinones were identified as MK-7 (56.4 %) and MK-8 (35.7 %).
32065575	0	24	theme	flavidum	16:23	arg1	sp					25:26	Exiguobacterium flavidum sp	0:26	Exiguobacterium flavidum sp.	0:27	Exiguobacterium flavidum sp.
32065575	9	25	theme	novel	1213:1217	arg1	species					1219:1225	a novel species	1211:1225	a novel species	1211:1225	The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness indicated that strain HF60T can be considered to represent a novel species of the genus Exiguobacterium, for which the name Exiguobacterium flavidum sp.
32065575	8	26	theme	Exiguobacterium	951:965	arg1	14480T					983:988	Exiguobacterium antarcticum DSM 14480T	951:988	Exiguobacterium antarcticum DSM 14480T (96.9 %)	951:997	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	8	26	theme	Exiguobacterium	951:965	arg1	%					996:996	96.9 %	991:996	96.9 %	991:996	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	8	27	dep	Exiguobacterium	951:965	arg1	antarcticum					967:977	antarcticum	967:977	antarcticum	967:977	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	0	28	theme	Exiguobacterium	0:14	arg1	sp					25:26	Exiguobacterium flavidum sp	0:26	Exiguobacterium flavidum sp.	0:27	Exiguobacterium flavidum sp.
32065575	8	29	dep	Exiguobacterium	1003:1017	arg1	aurantiacum					1019:1029	aurantiacum	1019:1029	aurantiacum	1019:1029	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	8	30	theme	DSM	979:981	arg1	14480T					983:988	Exiguobacterium antarcticum DSM 14480T	951:988	Exiguobacterium antarcticum DSM 14480T (96.9 %)	951:997	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	8	30	theme	DSM	979:981	arg1	%					996:996	96.9 %	991:996	96.9 %	991:996	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	4	31	theme	isoprenoid	322:331	arg1	MK-8					379:382	MK-8	379:382	MK-8	379:382	The predominant isoprenoid quinones were identified as MK-7 (56.4 %) and MK-8 (35.7 %).
32065575	4	31	theme	isoprenoid	322:331	arg1	quinones					333:340	The predominant isoprenoid quinones	306:340	The predominant isoprenoid quinones	306:340	The predominant isoprenoid quinones were identified as MK-7 (56.4 %) and MK-8 (35.7 %).
32065575	4	31	theme	isoprenoid	322:331	arg1	MK-7					361:364	MK-7	361:364	MK-7	361:364	The predominant isoprenoid quinones were identified as MK-7 (56.4 %) and MK-8 (35.7 %).
32065575	9	32	dep	Exiguobacterium	1276:1290	arg1	flavidum					1292:1299	flavidum	1292:1299	flavidum	1292:1299	The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness indicated that strain HF60T can be considered to represent a novel species of the genus Exiguobacterium, for which the name Exiguobacterium flavidum sp.
32065575	8	33	dep	Exiguobacterium	908:922	arg1	undae					924:928	undae	924:928	undae	924:928	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	10	34	theme	1H00336T=KCTC	1355:1367	arg1	33987T					1369:1374	=MCCC 1H00336T=KCTC 33987T	1349:1374	=MCCC 1H00336T=KCTC 33987T	1349:1374	nov. is proposed, The type strain is HF60T (=MCCC 1H00336T=KCTC 33987T).
32065575	10	34	theme	1H00336T=KCTC	1355:1367	arg1	HF60T					1342:1346	HF60T	1342:1346	HF60T (=MCCC 1H00336T=KCTC 33987T)	1342:1375	nov. is proposed, The type strain is HF60T (=MCCC 1H00336T=KCTC 33987T).
32065575	9	35	theme	phylogenetic	1111:1122	arg1	distinctiveness					1136:1150	the phylogenetic and genetic distinctiveness	1107:1150	the phylogenetic and genetic distinctiveness	1107:1150	The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness indicated that strain HF60T can be considered to represent a novel species of the genus Exiguobacterium, for which the name Exiguobacterium flavidum sp.
32065575	2	36	theme	Red	211:213	arg1	Lake					221:224	the Red Maple Lake	207:224	the Red Maple Lake of Guizhou Province, China	207:251	A Gram-stain-positive, motile, facultatively anaerobic, non-sporing, and rod-shaped bacterial strain, designated HF60T, was isolated from the Red Maple Lake of Guizhou Province, China.
32065575	8	37	theme	Exiguobacterium	815:829	arg1	13490T					845:850	Exiguobacterium sibiricum JCM 13490T	815:850	Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity)	815:893	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	8	37	theme	Exiguobacterium	815:829	arg1	similarity					883:892	97.2 % 16S rRNA gene sequence similarity	853:892	97.2 % 16S rRNA gene sequence similarity	853:892	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	7	38	contain	had	614:616	arg2	l-Lys-Gly					651:659	cell wall peptidoglycan type A3α l-Lys-Gly	618:659	cell wall peptidoglycan type A3α l-Lys-Gly	618:659	The strain had cell wall peptidoglycan type A3α l-Lys-Gly.
32065575	7	38	contain	had	614:616	arg1	strain					607:612	The strain	603:612	The strain	603:612	The strain had cell wall peptidoglycan type A3α l-Lys-Gly.
32065575	6	39	theme	fatty	531:535	arg1	anteiso-C13 					548:559	anteiso-C13 	548:559	anteiso-C13 	548:559	The major fatty acids were anteiso-C13 : 0, iso-C15 : 0, C16 : 0 and iso-C13 : 0.
32065575	6	39	theme	fatty	531:535	arg1	acids					537:541	The major fatty acids	521:541	The major fatty acids	521:541	The major fatty acids were anteiso-C13 : 0, iso-C15 : 0, C16 : 0 and iso-C13 : 0.
32065575	9	40	theme	Exiguobacterium	1240:1254	arg1	species					1219:1225	a novel species	1211:1225	a novel species	1211:1225	The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness indicated that strain HF60T can be considered to represent a novel species of the genus Exiguobacterium, for which the name Exiguobacterium flavidum sp.
32065575	8	41	theme	rRNA	697:700	arg1	sequences					707:715	16S rRNA gene sequences	693:715	16S rRNA gene sequences	693:715	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	2	42	theme	bacterial	153:161	arg1	strain					163:168	A Gram-stain-positive, motile, facultatively anaerobic, non-sporing, and rod-shaped bacterial strain	69:168	A Gram-stain-positive, motile, facultatively anaerobic, non-sporing, and rod-shaped bacterial strain	69:168	A Gram-stain-positive, motile, facultatively anaerobic, non-sporing, and rod-shaped bacterial strain, designated HF60T, was isolated from the Red Maple Lake of Guizhou Province, China.
32065575	8	43	theme	16S	693:695	arg1	sequences					707:715	16S rRNA gene sequences	693:715	16S rRNA gene sequences	693:715	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	6	44	theme	major	525:529	arg1	anteiso-C13 					548:559	anteiso-C13 	548:559	anteiso-C13 	548:559	The major fatty acids were anteiso-C13 : 0, iso-C15 : 0, C16 : 0 and iso-C13 : 0.
32065575	6	44	theme	major	525:529	arg1	acids					537:541	The major fatty acids	521:541	The major fatty acids	521:541	The major fatty acids were anteiso-C13 : 0, iso-C15 : 0, C16 : 0 and iso-C13 : 0.
32065575	2	45	theme	Gram-stain-positive	71:89	arg1	strain					163:168	A Gram-stain-positive, motile, facultatively anaerobic, non-sporing, and rod-shaped bacterial strain	69:168	A Gram-stain-positive, motile, facultatively anaerobic, non-sporing, and rod-shaped bacterial strain	69:168	A Gram-stain-positive, motile, facultatively anaerobic, non-sporing, and rod-shaped bacterial strain, designated HF60T, was isolated from the Red Maple Lake of Guizhou Province, China.
32065575	3	46	theme	G+C	262:264	arg1	%					303:303	55.0 %	298:303	55.0 %	298:303	The DNA G+C content of the strain HF60T was 55.0 %.
32065575	3	46	theme	G+C	262:264	arg1	content					266:272	The DNA G+C content	254:272	The DNA G+C content of the strain HF60T	254:292	The DNA G+C content of the strain HF60T was 55.0 %.
32065575	2	47	theme	rod-shaped	142:151	arg1	strain					163:168	A Gram-stain-positive, motile, facultatively anaerobic, non-sporing, and rod-shaped bacterial strain	69:168	A Gram-stain-positive, motile, facultatively anaerobic, non-sporing, and rod-shaped bacterial strain	69:168	A Gram-stain-positive, motile, facultatively anaerobic, non-sporing, and rod-shaped bacterial strain, designated HF60T, was isolated from the Red Maple Lake of Guizhou Province, China.
32065575	8	48	theme	JCM	841:843	arg1	13490T					845:850	Exiguobacterium sibiricum JCM 13490T	815:850	Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity)	815:893	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	8	48	theme	JCM	841:843	arg1	similarity					883:892	97.2 % 16S rRNA gene sequence similarity	853:892	97.2 % 16S rRNA gene sequence similarity	853:892	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	10	49	theme	=MCCC	1349:1353	arg1	33987T					1369:1374	=MCCC 1H00336T=KCTC 33987T	1349:1374	=MCCC 1H00336T=KCTC 33987T	1349:1374	nov. is proposed, The type strain is HF60T (=MCCC 1H00336T=KCTC 33987T).
32065575	10	49	theme	=MCCC	1349:1353	arg1	HF60T					1342:1346	HF60T	1342:1346	HF60T (=MCCC 1H00336T=KCTC 33987T)	1342:1375	nov. is proposed, The type strain is HF60T (=MCCC 1H00336T=KCTC 33987T).
32065575	8	50	theme	Phylogenetic	662:673	arg1	analyses					675:682	Phylogenetic analyses	662:682	Phylogenetic analyses based on 16S rRNA gene sequences	662:715	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	8	51	theme	97.2 	853:857	arg1	similarity					883:892	97.2 % 16S rRNA gene sequence similarity	853:892	97.2 % 16S rRNA gene sequence similarity	853:892	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	8	51	theme	97.2 	853:857	arg1	13490T					845:850	Exiguobacterium sibiricum JCM 13490T	815:850	Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity)	815:893	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	8	52	theme	strain	732:737	arg1	HF60T					739:743	strain HF60T	732:743	strain HF60T	732:743	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	8	53	theme	Exiguobacterium	908:922	arg1	14481T					934:939	Exiguobacterium undae DSM 14481T	908:939	Exiguobacterium undae DSM 14481T (97.1 %)	908:948	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	8	53	theme	Exiguobacterium	908:922	arg1	%					947:947	97.1 %	942:947	97.1 %	942:947	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	6	54	dep	 0	561:562	arg1	C16 					578:581	C16 	578:581	C16 	578:581	The major fatty acids were anteiso-C13 : 0, iso-C15 : 0, C16 : 0 and iso-C13 : 0.
32065575	6	54	dep	 0	561:562	arg1	 0					599:600	 0	599:600	 0	599:600	The major fatty acids were anteiso-C13 : 0, iso-C15 : 0, C16 : 0 and iso-C13 : 0.
32065575	6	54	dep	 0	561:562	arg1	iso-C13 					590:597	iso-C13 	590:597	iso-C13 	590:597	The major fatty acids were anteiso-C13 : 0, iso-C15 : 0, C16 : 0 and iso-C13 : 0.
32065575	6	54	dep	 0	561:562	arg1	 0					583:584	 0	583:584	 0	583:584	The major fatty acids were anteiso-C13 : 0, iso-C15 : 0, C16 : 0 and iso-C13 : 0.
32065575	9	55	theme	genus	1234:1238	arg1	Exiguobacterium					1240:1254	the genus Exiguobacterium	1230:1254	the genus Exiguobacterium	1230:1254	The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness indicated that strain HF60T can be considered to represent a novel species of the genus Exiguobacterium, for which the name Exiguobacterium flavidum sp.
32065575	8	56	dep	Exiguobacterium	815:829	arg1	sibiricum					831:839	sibiricum	831:839	sibiricum	831:839	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	3	57	theme	DNA	258:260	arg1	%					303:303	55.0 %	298:303	55.0 %	298:303	The DNA G+C content of the strain HF60T was 55.0 %.
32065575	3	57	theme	DNA	258:260	arg1	content					266:272	The DNA G+C content	254:272	The DNA G+C content of the strain HF60T	254:292	The DNA G+C content of the strain HF60T was 55.0 %.
32065575	8	58	theme	16S	860:862	arg1	similarity					883:892	97.2 % 16S rRNA gene sequence similarity	853:892	97.2 % 16S rRNA gene sequence similarity	853:892	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	8	58	theme	16S	860:862	arg1	13490T					845:850	Exiguobacterium sibiricum JCM 13490T	815:850	Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity)	815:893	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	9	59	theme	phenotypic	1070:1079	arg1	properties					1081:1090	The differential phenotypic properties	1053:1090	The differential phenotypic properties	1053:1090	The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness indicated that strain HF60T can be considered to represent a novel species of the genus Exiguobacterium, for which the name Exiguobacterium flavidum sp.
32065575	9	60	theme	name	1271:1274	arg1	sp					1301:1302	the name Exiguobacterium flavidum sp	1267:1302	the name Exiguobacterium flavidum sp	1267:1302	The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness indicated that strain HF60T can be considered to represent a novel species of the genus Exiguobacterium, for which the name Exiguobacterium flavidum sp.
32065575	3	61	theme	strain	281:286	arg1	HF60T					288:292	the strain HF60T	277:292	the strain HF60T	277:292	The DNA G+C content of the strain HF60T was 55.0 %.
32065575	9	62	theme	strain	1167:1172	arg1	HF60T					1174:1178	strain HF60T	1167:1178	strain HF60T	1167:1178	The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness indicated that strain HF60T can be considered to represent a novel species of the genus Exiguobacterium, for which the name Exiguobacterium flavidum sp.
32065575	8	63	theme	%	858:858	arg1	similarity					883:892	97.2 % 16S rRNA gene sequence similarity	853:892	97.2 % 16S rRNA gene sequence similarity	853:892	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	8	63	theme	%	858:858	arg1	13490T					845:850	Exiguobacterium sibiricum JCM 13490T	815:850	Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity)	815:893	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	7	64	theme	cell	618:621	arg1	l-Lys-Gly					651:659	cell wall peptidoglycan type A3α l-Lys-Gly	618:659	cell wall peptidoglycan type A3α l-Lys-Gly	618:659	The strain had cell wall peptidoglycan type A3α l-Lys-Gly.
32065575	7	65	theme	A3α	647:649	arg1	l-Lys-Gly					651:659	cell wall peptidoglycan type A3α l-Lys-Gly	618:659	cell wall peptidoglycan type A3α l-Lys-Gly	618:659	The strain had cell wall peptidoglycan type A3α l-Lys-Gly.
32065575	3	66	theme	HF60T	288:292	arg1	%					303:303	55.0 %	298:303	55.0 %	298:303	The DNA G+C content of the strain HF60T was 55.0 %.
32065575	3	66	theme	HF60T	288:292	arg1	content					266:272	The DNA G+C content	254:272	The DNA G+C content of the strain HF60T	254:292	The DNA G+C content of the strain HF60T was 55.0 %.
32065575	2	67	theme	anaerobic	114:122	arg1	strain					163:168	A Gram-stain-positive, motile, facultatively anaerobic, non-sporing, and rod-shaped bacterial strain	69:168	A Gram-stain-positive, motile, facultatively anaerobic, non-sporing, and rod-shaped bacterial strain	69:168	A Gram-stain-positive, motile, facultatively anaerobic, non-sporing, and rod-shaped bacterial strain, designated HF60T, was isolated from the Red Maple Lake of Guizhou Province, China.
32065575	2	68	theme	Maple	215:219	arg1	Lake					221:224	the Red Maple Lake	207:224	the Red Maple Lake of Guizhou Province, China	207:251	A Gram-stain-positive, motile, facultatively anaerobic, non-sporing, and rod-shaped bacterial strain, designated HF60T, was isolated from the Red Maple Lake of Guizhou Province, China.
32065575	8	69	theme	gene	869:872	arg1	similarity					883:892	97.2 % 16S rRNA gene sequence similarity	853:892	97.2 % 16S rRNA gene sequence similarity	853:892	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	8	69	theme	gene	869:872	arg1	13490T					845:850	Exiguobacterium sibiricum JCM 13490T	815:850	Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity)	815:893	Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481T (97.1 %), Exiguobacterium antarcticum DSM 14480T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763T (94.5 %).
32065575	9	70	dep	together	1093:1100	arg1	with					1102:1105	with	1102:1105	with	1102:1105	The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness indicated that strain HF60T can be considered to represent a novel species of the genus Exiguobacterium, for which the name Exiguobacterium flavidum sp.
31724934	2	0	theme	A-T	114:116	arg1	5661T					118:122	strain A-T 5661T	107:122	strain A-T 5661T	107:122	A novel zoosporic actinomycete, designated strain A-T 5661T, was isolated from plant litter collected in Thailand.
31724934	3	1	theme	large	206:210	arg1	sporangia					225:233	large multilocular sporangia	206:233	large multilocular sporangia containing motile sporangiospores at the ends of sporangiophores on substrate mycelium	206:320	Strain A-T 5661T developed large multilocular sporangia containing motile sporangiospores at the ends of sporangiophores on substrate mycelium.
31724934	8	2	theme	whole-cell	829:838	arg1	acofriose					852:860	acofriose	852:860	acofriose	852:860	The whole-cell sugars were acofriose, arabinose, galactose, glucose, mannose, xylose and ribose.
31724934	8	2	theme	whole-cell	829:838	arg1	sugars					840:845	The whole-cell sugars	825:845	The whole-cell sugars	825:845	The whole-cell sugars were acofriose, arabinose, galactose, glucose, mannose, xylose and ribose.
31724934	10	3	theme	predominant	1027:1037	arg1	iso-C16 					1056:1063	iso-C16 	1056:1063	iso-C16 	1056:1063	The predominant fatty acids were iso-C16 : 0, C18 : 1ω9c and anteiso-C17 : 0.
31724934	10	3	theme	predominant	1027:1037	arg1	acids					1045:1049	The predominant fatty acids	1023:1049	The predominant fatty acids	1023:1049	The predominant fatty acids were iso-C16 : 0, C18 : 1ω9c and anteiso-C17 : 0.
31724934	2	4	theme	strain	107:112	arg1	5661T					118:122	strain A-T 5661T	107:122	strain A-T 5661T	107:122	A novel zoosporic actinomycete, designated strain A-T 5661T, was isolated from plant litter collected in Thailand.
31724934	3	5	theme	multilocular	212:223	arg1	sporangia					225:233	large multilocular sporangia	206:233	large multilocular sporangia containing motile sporangiospores at the ends of sporangiophores on substrate mycelium	206:320	Strain A-T 5661T developed large multilocular sporangia containing motile sporangiospores at the ends of sporangiophores on substrate mycelium.
31724934	13	6	theme	genotypic	1409:1417	arg1	data					1419:1422	phenotypic, chemotaxonomic and genotypic data	1378:1422	phenotypic, chemotaxonomic and genotypic data	1378:1422	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T) is proposed to be the type strain of a novel species, Cryptosporangium phraense sp.
31724934	11	7	theme	phosphatidylinositol	1232:1251	arg1	mannosides					1253:1262	phosphatidylinositol mannosides	1232:1262	phosphatidylinositol mannosides	1232:1262	The polar lipid profile consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and unidentified polar lipids.
31724934	13	8	theme	type	1517:1520	arg1	5661T					1462:1466	the novel zoosporic actinomycete A-T 5661T	1425:1466	the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T)	1425:1493	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T) is proposed to be the type strain of a novel species, Cryptosporangium phraense sp.
31724934	13	8	theme	type	1517:1520	arg1	strain					1522:1527	the type strain	1513:1527	the type strain of a novel species, Cryptosporangium phraense sp	1513:1576	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T) is proposed to be the type strain of a novel species, Cryptosporangium phraense sp.
31724934	1	9	theme	plant	50:54	arg1	litter					56:61	plant litter	50:61	plant litter	50:61	nov., isolated from plant litter.
31724934	3	10	from	ends	276:279	arg1	mycelium					313:320	substrate mycelium	303:320	substrate mycelium	303:320	Strain A-T 5661T developed large multilocular sporangia containing motile sporangiospores at the ends of sporangiophores on substrate mycelium.
31724934	11	11	theme	polar	1105:1109	arg1	profile					1117:1123	The polar lipid profile	1101:1123	The polar lipid profile	1101:1123	The polar lipid profile consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and unidentified polar lipids.
31724934	5	12	theme	genus	616:620	arg1	Cryptosporangium					622:637	the genus Cryptosporangium	612:637	the genus Cryptosporangium	612:637	The 16S rRNA gene sequence and phylogenetic analyses indicated that strain A-T 5661T shared the sequence similarity ≤98.5 % with all members of the genus Cryptosporangium.
31724934	7	13	contain	contained	789:797	arg2	acid					819:822	meso-diaminopimelic acid	799:822	meso-diaminopimelic acid	799:822	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
31724934	7	13	contain	contained	789:797	arg1	peptidoglycan					775:787	The cell-wall peptidoglycan	761:787	The cell-wall peptidoglycan	761:787	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
31724934	5	14	theme	phylogenetic	499:510	arg1	analyses					512:519	phylogenetic analyses	499:519	phylogenetic analyses	499:519	The 16S rRNA gene sequence and phylogenetic analyses indicated that strain A-T 5661T shared the sequence similarity ≤98.5 % with all members of the genus Cryptosporangium.
31724934	5	15	dep	similarity	573:582	arg1	%					590:590	≤98.5 %	584:590	the sequence similarity ≤98.5 %	560:590	The 16S rRNA gene sequence and phylogenetic analyses indicated that strain A-T 5661T shared the sequence similarity ≤98.5 % with all members of the genus Cryptosporangium.
31724934	3	16	theme	motile	246:251	arg1	sporangiospores					253:267	motile sporangiospores	246:267	motile sporangiospores	246:267	Strain A-T 5661T developed large multilocular sporangia containing motile sporangiospores at the ends of sporangiophores on substrate mycelium.
31724934	4	17	theme	typical	418:424	arg1	characteristics					402:416	characteristics	402:416	characteristics typical of members of the genus Cryptosporangium	402:465	The results of our polyphasic taxonomic study demonstrated that the strain had characteristics typical of members of the genus Cryptosporangium.
31724934	13	18	theme	phraense	1566:1573	arg1	sp					1575:1576	phraense sp	1566:1576	phraense sp	1566:1576	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T) is proposed to be the type strain of a novel species, Cryptosporangium phraense sp.
31724934	6	19	theme	related	728:734	arg1	species					736:742	its closest related species	716:742	its closest related species	716:742	The values of DNA-DNA relatedness that distinguished this novel strain from its closest related species were below 70 %.
31724934	3	20	theme	Strain	179:184	arg1	5661T					190:194	Strain A-T 5661T	179:194	Strain A-T 5661T	179:194	Strain A-T 5661T developed large multilocular sporangia containing motile sporangiospores at the ends of sporangiophores on substrate mycelium.
31724934	6	21	theme	closest	720:726	arg1	species					736:742	its closest related species	716:742	its closest related species	716:742	The values of DNA-DNA relatedness that distinguished this novel strain from its closest related species were below 70 %.
31724934	13	22	theme	=TBRC	1469:1473	arg1	5661T					1462:1466	the novel zoosporic actinomycete A-T 5661T	1425:1466	the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T)	1425:1493	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T) is proposed to be the type strain of a novel species, Cryptosporangium phraense sp.
31724934	13	22	theme	=TBRC	1469:1473	arg1	113237T					1486:1492	=TBRC 8127T=NBRC 113237T	1469:1492	=TBRC 8127T=NBRC 113237T	1469:1492	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T) is proposed to be the type strain of a novel species, Cryptosporangium phraense sp.
31724934	13	23	theme	phenotypic	1378:1387	arg1	data					1419:1422	phenotypic, chemotaxonomic and genotypic data	1378:1422	phenotypic, chemotaxonomic and genotypic data	1378:1422	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T) is proposed to be the type strain of a novel species, Cryptosporangium phraense sp.
31724934	11	24	theme	polar	1281:1285	arg1	lipids					1287:1292	unidentified polar lipids	1268:1292	unidentified polar lipids	1268:1292	The polar lipid profile consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and unidentified polar lipids.
31724934	3	25	theme	A-T	186:188	arg1	5661T					190:194	Strain A-T 5661T	179:194	Strain A-T 5661T	179:194	Strain A-T 5661T developed large multilocular sporangia containing motile sporangiospores at the ends of sporangiophores on substrate mycelium.
31724934	13	26	theme	8127T=NBRC	1475:1484	arg1	5661T					1462:1466	the novel zoosporic actinomycete A-T 5661T	1425:1466	the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T)	1425:1493	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T) is proposed to be the type strain of a novel species, Cryptosporangium phraense sp.
31724934	13	26	theme	8127T=NBRC	1475:1484	arg1	113237T					1486:1492	=TBRC 8127T=NBRC 113237T	1469:1492	=TBRC 8127T=NBRC 113237T	1469:1492	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T) is proposed to be the type strain of a novel species, Cryptosporangium phraense sp.
31724934	13	27	theme	comparative	1354:1364	arg1	analysis					1366:1373	comparative analysis	1354:1373	comparative analysis of phenotypic, chemotaxonomic and genotypic data	1354:1422	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T) is proposed to be the type strain of a novel species, Cryptosporangium phraense sp.
31724934	5	28	theme	rRNA	476:479	arg1	sequence					486:493	The 16S rRNA gene sequence	468:493	The 16S rRNA gene sequence	468:493	The 16S rRNA gene sequence and phylogenetic analyses indicated that strain A-T 5661T shared the sequence similarity ≤98.5 % with all members of the genus Cryptosporangium.
31724934	11	29	theme	unidentified	1268:1279	arg1	lipids					1287:1292	unidentified polar lipids	1268:1292	unidentified polar lipids	1268:1292	The polar lipid profile consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and unidentified polar lipids.
31724934	5	30	theme	Cryptosporangium	622:637	arg1	members					601:607	all members	597:607	all members of the genus Cryptosporangium	597:637	The 16S rRNA gene sequence and phylogenetic analyses indicated that strain A-T 5661T shared the sequence similarity ≤98.5 % with all members of the genus Cryptosporangium.
31724934	11	31	theme	lipid	1111:1115	arg1	profile					1117:1123	The polar lipid profile	1101:1123	The polar lipid profile	1101:1123	The polar lipid profile consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and unidentified polar lipids.
31724934	5	32	theme	gene	481:484	arg1	sequence					486:493	The 16S rRNA gene sequence	468:493	The 16S rRNA gene sequence	468:493	The 16S rRNA gene sequence and phylogenetic analyses indicated that strain A-T 5661T shared the sequence similarity ≤98.5 % with all members of the genus Cryptosporangium.
31724934	13	33	theme	zoosporic	1435:1443	arg1	5661T					1462:1466	the novel zoosporic actinomycete A-T 5661T	1425:1466	the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T)	1425:1493	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T) is proposed to be the type strain of a novel species, Cryptosporangium phraense sp.
31724934	13	33	theme	zoosporic	1435:1443	arg1	strain					1522:1527	the type strain	1513:1527	the type strain of a novel species, Cryptosporangium phraense sp	1513:1576	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T) is proposed to be the type strain of a novel species, Cryptosporangium phraense sp.
31724934	13	33	theme	zoosporic	1435:1443	arg1	113237T					1486:1492	=TBRC 8127T=NBRC 113237T	1469:1492	=TBRC 8127T=NBRC 113237T	1469:1492	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T) is proposed to be the type strain of a novel species, Cryptosporangium phraense sp.
31724934	13	34	theme	chemotaxonomic	1390:1403	arg1	data					1419:1422	phenotypic, chemotaxonomic and genotypic data	1378:1422	phenotypic, chemotaxonomic and genotypic data	1378:1422	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T) is proposed to be the type strain of a novel species, Cryptosporangium phraense sp.
31724934	13	35	theme	species	1540:1546	arg1	5661T					1462:1466	the novel zoosporic actinomycete A-T 5661T	1425:1466	the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T)	1425:1493	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T) is proposed to be the type strain of a novel species, Cryptosporangium phraense sp.
31724934	13	35	theme	species	1540:1546	arg1	strain					1522:1527	the type strain	1513:1527	the type strain of a novel species, Cryptosporangium phraense sp	1513:1576	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T) is proposed to be the type strain of a novel species, Cryptosporangium phraense sp.
31724934	6	36	theme	novel	698:702	arg1	strain					704:709	this novel strain	693:709	this novel strain	693:709	The values of DNA-DNA relatedness that distinguished this novel strain from its closest related species were below 70 %.
31724934	13	37	theme	actinomycete	1445:1456	arg1	5661T					1462:1466	the novel zoosporic actinomycete A-T 5661T	1425:1466	the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T)	1425:1493	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T) is proposed to be the type strain of a novel species, Cryptosporangium phraense sp.
31724934	13	37	theme	actinomycete	1445:1456	arg1	strain					1522:1527	the type strain	1513:1527	the type strain of a novel species, Cryptosporangium phraense sp	1513:1576	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T) is proposed to be the type strain of a novel species, Cryptosporangium phraense sp.
31724934	13	37	theme	actinomycete	1445:1456	arg1	113237T					1486:1492	=TBRC 8127T=NBRC 113237T	1469:1492	=TBRC 8127T=NBRC 113237T	1469:1492	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T) is proposed to be the type strain of a novel species, Cryptosporangium phraense sp.
31724934	9	38	theme	minor	983:987	arg1	amounts					989:995	minor amounts	983:995	minor amounts of MK-9(H4) and MK-9(H2)	983:1020	The predominant menaquinones were MK-9(H8) and MK-9(H6) with minor amounts of MK-9(H4) and MK-9(H2).
31724934	9	38	theme	minor	983:987	arg1	H2					1018:1019	H2	1018:1019	H2	1018:1019	The predominant menaquinones were MK-9(H8) and MK-9(H6) with minor amounts of MK-9(H4) and MK-9(H2).
31724934	9	38	theme	minor	983:987	arg1	H4					1005:1006	H4	1005:1006	H4	1005:1006	The predominant menaquinones were MK-9(H8) and MK-9(H6) with minor amounts of MK-9(H4) and MK-9(H2).
31724934	9	38	theme	minor	983:987	arg1	MK-9					1013:1016	MK-9	1013:1016	MK-9(H2)	1013:1020	The predominant menaquinones were MK-9(H8) and MK-9(H6) with minor amounts of MK-9(H4) and MK-9(H2).
31724934	9	38	theme	minor	983:987	arg1	MK-9					1000:1003	MK-9	1000:1003	MK-9(H4)	1000:1007	The predominant menaquinones were MK-9(H8) and MK-9(H6) with minor amounts of MK-9(H4) and MK-9(H2).
31724934	10	39	dep	iso-C16 	1056:1063	arg1	C18 					1069:1072	C18 	1069:1072	C18 	1069:1072	The predominant fatty acids were iso-C16 : 0, C18 : 1ω9c and anteiso-C17 : 0.
31724934	10	39	dep	iso-C16 	1056:1063	arg1	 0					1097:1098	 0	1097:1098	 0	1097:1098	The predominant fatty acids were iso-C16 : 0, C18 : 1ω9c and anteiso-C17 : 0.
31724934	10	39	dep	iso-C16 	1056:1063	arg1	anteiso-C17 					1084:1095	anteiso-C17 	1084:1095	anteiso-C17 	1084:1095	The predominant fatty acids were iso-C16 : 0, C18 : 1ω9c and anteiso-C17 : 0.
31724934	10	39	dep	iso-C16 	1056:1063	arg1	 1ω9c					1074:1078	 1ω9c	1074:1078	 1ω9c	1074:1078	The predominant fatty acids were iso-C16 : 0, C18 : 1ω9c and anteiso-C17 : 0.
31724934	4	40	theme	Cryptosporangium	450:465	arg1	members					429:435	members	429:435	members of the genus Cryptosporangium	429:465	The results of our polyphasic taxonomic study demonstrated that the strain had characteristics typical of members of the genus Cryptosporangium.
31724934	12	41	theme	DNA	1326:1328	arg1	%					1342:1342	71.6 mol%	1334:1342	71.6 mol%	1334:1342	The G+C content of the genomic DNA was 71.6 mol%.
31724934	12	41	theme	DNA	1326:1328	arg1	content					1303:1309	The G+C content	1295:1309	The G+C content of the genomic DNA	1295:1328	The G+C content of the genomic DNA was 71.6 mol%.
31724934	6	42	theme	DNA-DNA	654:660	arg1	relatedness					662:672	DNA-DNA relatedness	654:672	DNA-DNA relatedness that distinguished this novel strain from its closest related species	654:742	The values of DNA-DNA relatedness that distinguished this novel strain from its closest related species were below 70 %.
31724934	13	43	theme	A-T	1458:1460	arg1	5661T					1462:1466	the novel zoosporic actinomycete A-T 5661T	1425:1466	the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T)	1425:1493	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T) is proposed to be the type strain of a novel species, Cryptosporangium phraense sp.
31724934	13	43	theme	A-T	1458:1460	arg1	strain					1522:1527	the type strain	1513:1527	the type strain of a novel species, Cryptosporangium phraense sp	1513:1576	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T) is proposed to be the type strain of a novel species, Cryptosporangium phraense sp.
31724934	13	43	theme	A-T	1458:1460	arg1	113237T					1486:1492	=TBRC 8127T=NBRC 113237T	1469:1492	=TBRC 8127T=NBRC 113237T	1469:1492	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T) is proposed to be the type strain of a novel species, Cryptosporangium phraense sp.
31724934	12	44	theme	genomic	1318:1324	arg1	DNA					1326:1328	the genomic DNA	1314:1328	the genomic DNA	1314:1328	The G+C content of the genomic DNA was 71.6 mol%.
31724934	4	45	theme	polyphasic	342:351	arg1	study					363:367	our polyphasic taxonomic study	338:367	our polyphasic taxonomic study	338:367	The results of our polyphasic taxonomic study demonstrated that the strain had characteristics typical of members of the genus Cryptosporangium.
31724934	7	46	theme	meso-diaminopimelic	799:817	arg1	acid					819:822	meso-diaminopimelic acid	799:822	meso-diaminopimelic acid	799:822	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
31724934	4	47	contain	had	398:400	arg2	characteristics					402:416	characteristics	402:416	characteristics typical of members of the genus Cryptosporangium	402:465	The results of our polyphasic taxonomic study demonstrated that the strain had characteristics typical of members of the genus Cryptosporangium.
31724934	4	47	contain	had	398:400	arg1	strain					391:396	the strain	387:396	the strain	387:396	The results of our polyphasic taxonomic study demonstrated that the strain had characteristics typical of members of the genus Cryptosporangium.
31724934	3	48	theme	substrate	303:311	arg1	mycelium					313:320	substrate mycelium	303:320	substrate mycelium	303:320	Strain A-T 5661T developed large multilocular sporangia containing motile sporangiospores at the ends of sporangiophores on substrate mycelium.
31724934	2	49	theme	plant	143:147	arg1	litter					149:154	plant litter	143:154	plant litter collected in Thailand	143:176	A novel zoosporic actinomycete, designated strain A-T 5661T, was isolated from plant litter collected in Thailand.
31724934	12	50	theme	71.6 mol	1334:1341	arg1	%					1342:1342	71.6 mol%	1334:1342	71.6 mol%	1334:1342	The G+C content of the genomic DNA was 71.6 mol%.
31724934	12	50	theme	71.6 mol	1334:1341	arg1	content					1303:1309	The G+C content	1295:1309	The G+C content of the genomic DNA	1295:1328	The G+C content of the genomic DNA was 71.6 mol%.
31724934	5	51	theme	sequence	564:571	arg1	similarity					573:582	the sequence similarity	560:582	the sequence similarity ≤98.5 %	560:590	The 16S rRNA gene sequence and phylogenetic analyses indicated that strain A-T 5661T shared the sequence similarity ≤98.5 % with all members of the genus Cryptosporangium.
31724934	2	52	attach	isolated	129:136	arg2	actinomycete					82:93	A novel zoosporic actinomycete	64:93	A novel zoosporic actinomycete	64:93	A novel zoosporic actinomycete, designated strain A-T 5661T, was isolated from plant litter collected in Thailand.
31724934	2	52	attach	isolated	129:136	arg1	litter					149:154	plant litter	143:154	plant litter collected in Thailand	143:176	A novel zoosporic actinomycete, designated strain A-T 5661T, was isolated from plant litter collected in Thailand.
31724934	9	53	theme	MK-9	1000:1003	arg1	amounts					989:995	minor amounts	983:995	minor amounts of MK-9(H4) and MK-9(H2)	983:1020	The predominant menaquinones were MK-9(H8) and MK-9(H6) with minor amounts of MK-9(H4) and MK-9(H2).
31724934	9	53	theme	MK-9	1000:1003	arg1	H2					1018:1019	H2	1018:1019	H2	1018:1019	The predominant menaquinones were MK-9(H8) and MK-9(H6) with minor amounts of MK-9(H4) and MK-9(H2).
31724934	9	53	theme	MK-9	1000:1003	arg1	H4					1005:1006	H4	1005:1006	H4	1005:1006	The predominant menaquinones were MK-9(H8) and MK-9(H6) with minor amounts of MK-9(H4) and MK-9(H2).
31724934	9	53	theme	MK-9	1000:1003	arg1	MK-9					1013:1016	MK-9	1013:1016	MK-9(H2)	1013:1020	The predominant menaquinones were MK-9(H8) and MK-9(H6) with minor amounts of MK-9(H4) and MK-9(H2).
31724934	9	53	theme	MK-9	1000:1003	arg1	MK-9					1000:1003	MK-9	1000:1003	MK-9(H4)	1000:1007	The predominant menaquinones were MK-9(H8) and MK-9(H6) with minor amounts of MK-9(H4) and MK-9(H2).
31724934	2	54	theme	zoosporic	72:80	arg1	actinomycete					82:93	A novel zoosporic actinomycete	64:93	A novel zoosporic actinomycete	64:93	A novel zoosporic actinomycete, designated strain A-T 5661T, was isolated from plant litter collected in Thailand.
31724934	13	55	theme	data	1419:1422	arg1	analysis					1366:1373	comparative analysis	1354:1373	comparative analysis of phenotypic, chemotaxonomic and genotypic data	1354:1422	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T) is proposed to be the type strain of a novel species, Cryptosporangium phraense sp.
31724934	4	56	theme	study	363:367	arg1	results					327:333	The results	323:333	The results of our polyphasic taxonomic study	323:367	The results of our polyphasic taxonomic study demonstrated that the strain had characteristics typical of members of the genus Cryptosporangium.
31724934	13	57	dep	sp	1575:1576	arg1	Cryptosporangium					1549:1564	Cryptosporangium	1549:1564	Cryptosporangium	1549:1564	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T) is proposed to be the type strain of a novel species, Cryptosporangium phraense sp.
31724934	3	58	contain	containing	235:244	arg2	sporangiospores					253:267	motile sporangiospores	246:267	motile sporangiospores	246:267	Strain A-T 5661T developed large multilocular sporangia containing motile sporangiospores at the ends of sporangiophores on substrate mycelium.
31724934	3	58	contain	containing	235:244	arg1	sporangia					225:233	large multilocular sporangia	206:233	large multilocular sporangia containing motile sporangiospores at the ends of sporangiophores on substrate mycelium	206:320	Strain A-T 5661T developed large multilocular sporangia containing motile sporangiospores at the ends of sporangiophores on substrate mycelium.
31724934	2	59	theme	novel	66:70	arg1	actinomycete					82:93	A novel zoosporic actinomycete	64:93	A novel zoosporic actinomycete	64:93	A novel zoosporic actinomycete, designated strain A-T 5661T, was isolated from plant litter collected in Thailand.
31724934	3	60	theme	sporangiophores	284:298	arg1	ends					276:279	the ends	272:279	the ends of sporangiophores on substrate mycelium	272:320	Strain A-T 5661T developed large multilocular sporangia containing motile sporangiospores at the ends of sporangiophores on substrate mycelium.
31724934	4	61	theme	members	429:435	arg1	typical					418:424	typical	418:424	typical	418:424	The results of our polyphasic taxonomic study demonstrated that the strain had characteristics typical of members of the genus Cryptosporangium.
31724934	4	62	theme	taxonomic	353:361	arg1	study					363:367	our polyphasic taxonomic study	338:367	our polyphasic taxonomic study	338:367	The results of our polyphasic taxonomic study demonstrated that the strain had characteristics typical of members of the genus Cryptosporangium.
31724934	13	63	theme	novel	1534:1538	arg1	species					1540:1546	a novel species	1532:1546	a novel species	1532:1546	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T) is proposed to be the type strain of a novel species, Cryptosporangium phraense sp.
31724934	3	64	from	sporangiophores	284:298	arg1	mycelium					313:320	substrate mycelium	303:320	substrate mycelium	303:320	Strain A-T 5661T developed large multilocular sporangia containing motile sporangiospores at the ends of sporangiophores on substrate mycelium.
31724934	9	65	theme	predominant	926:936	arg1	MK-9					956:959	MK-9	956:959	MK-9	956:959	The predominant menaquinones were MK-9(H8) and MK-9(H6) with minor amounts of MK-9(H4) and MK-9(H2).
31724934	9	65	theme	predominant	926:936	arg1	menaquinones					938:949	The predominant menaquinones	922:949	The predominant menaquinones	922:949	The predominant menaquinones were MK-9(H8) and MK-9(H6) with minor amounts of MK-9(H4) and MK-9(H2).
31724934	4	66	theme	genus	444:448	arg1	Cryptosporangium					450:465	the genus Cryptosporangium	440:465	the genus Cryptosporangium	440:465	The results of our polyphasic taxonomic study demonstrated that the strain had characteristics typical of members of the genus Cryptosporangium.
31724934	12	67	theme	G+C	1299:1301	arg1	%					1342:1342	71.6 mol%	1334:1342	71.6 mol%	1334:1342	The G+C content of the genomic DNA was 71.6 mol%.
31724934	12	67	theme	G+C	1299:1301	arg1	content					1303:1309	The G+C content	1295:1309	The G+C content of the genomic DNA	1295:1328	The G+C content of the genomic DNA was 71.6 mol%.
31724934	5	68	theme	strain	536:541	arg1	5661T					547:551	strain A-T 5661T	536:551	strain A-T 5661T	536:551	The 16S rRNA gene sequence and phylogenetic analyses indicated that strain A-T 5661T shared the sequence similarity ≤98.5 % with all members of the genus Cryptosporangium.
31724934	13	69	theme	novel	1429:1433	arg1	5661T					1462:1466	the novel zoosporic actinomycete A-T 5661T	1425:1466	the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T)	1425:1493	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T) is proposed to be the type strain of a novel species, Cryptosporangium phraense sp.
31724934	13	69	theme	novel	1429:1433	arg1	strain					1522:1527	the type strain	1513:1527	the type strain of a novel species, Cryptosporangium phraense sp	1513:1576	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T) is proposed to be the type strain of a novel species, Cryptosporangium phraense sp.
31724934	13	69	theme	novel	1429:1433	arg1	113237T					1486:1492	=TBRC 8127T=NBRC 113237T	1469:1492	=TBRC 8127T=NBRC 113237T	1469:1492	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel zoosporic actinomycete A-T 5661T (=TBRC 8127T=NBRC 113237T) is proposed to be the type strain of a novel species, Cryptosporangium phraense sp.
31724934	6	70	theme	relatedness	662:672	arg1	values					644:649	The values	640:649	The values of DNA-DNA relatedness that distinguished this novel strain from its closest related species	640:742	The values of DNA-DNA relatedness that distinguished this novel strain from its closest related species were below 70 %.
31724934	5	71	theme	A-T	543:545	arg1	5661T					547:551	strain A-T 5661T	536:551	strain A-T 5661T	536:551	The 16S rRNA gene sequence and phylogenetic analyses indicated that strain A-T 5661T shared the sequence similarity ≤98.5 % with all members of the genus Cryptosporangium.
31724934	5	72	theme	16S	472:474	arg1	rRNA					476:479	The 16S rRNA	468:479	The 16S rRNA gene sequence	468:493	The 16S rRNA gene sequence and phylogenetic analyses indicated that strain A-T 5661T shared the sequence similarity ≤98.5 % with all members of the genus Cryptosporangium.
31724934	10	73	theme	fatty	1039:1043	arg1	iso-C16 					1056:1063	iso-C16 	1056:1063	iso-C16 	1056:1063	The predominant fatty acids were iso-C16 : 0, C18 : 1ω9c and anteiso-C17 : 0.
31724934	10	73	theme	fatty	1039:1043	arg1	acids					1045:1049	The predominant fatty acids	1023:1049	The predominant fatty acids	1023:1049	The predominant fatty acids were iso-C16 : 0, C18 : 1ω9c and anteiso-C17 : 0.
31724934	7	74	theme	cell-wall	765:773	arg1	peptidoglycan					775:787	The cell-wall peptidoglycan	761:787	The cell-wall peptidoglycan	761:787	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
31724934	9	75	theme	MK-9	1013:1016	arg1	amounts					989:995	minor amounts	983:995	minor amounts of MK-9(H4) and MK-9(H2)	983:1020	The predominant menaquinones were MK-9(H8) and MK-9(H6) with minor amounts of MK-9(H4) and MK-9(H2).
31724934	9	75	theme	MK-9	1013:1016	arg1	H2					1018:1019	H2	1018:1019	H2	1018:1019	The predominant menaquinones were MK-9(H8) and MK-9(H6) with minor amounts of MK-9(H4) and MK-9(H2).
31724934	9	75	theme	MK-9	1013:1016	arg1	H4					1005:1006	H4	1005:1006	H4	1005:1006	The predominant menaquinones were MK-9(H8) and MK-9(H6) with minor amounts of MK-9(H4) and MK-9(H2).
31724934	9	75	theme	MK-9	1013:1016	arg1	MK-9					1013:1016	MK-9	1013:1016	MK-9(H2)	1013:1020	The predominant menaquinones were MK-9(H8) and MK-9(H6) with minor amounts of MK-9(H4) and MK-9(H2).
31724934	9	75	theme	MK-9	1013:1016	arg1	MK-9					1000:1003	MK-9	1000:1003	MK-9(H4)	1000:1007	The predominant menaquinones were MK-9(H8) and MK-9(H6) with minor amounts of MK-9(H4) and MK-9(H2).
31724934	3	76	from	mycelium	313:320	arg1	ends					276:279	the ends	272:279	the ends of sporangiophores on substrate mycelium	272:320	Strain A-T 5661T developed large multilocular sporangia containing motile sporangiospores at the ends of sporangiophores on substrate mycelium.
32438363	3	0	theme	UV	712:713	arg1	nm					733:734	265 nm	729:734	265 nm	729:734	Methacrylated alginate solution was mixed with the required amount of surface-functionalized HAp nanoparticles in the presence of 0.05% Irgacure 2959 as a photoinitiator and was placed at the centre of a 8 kW UV light source (265 nm) to prepare photo-crosslinked bone paste.
32438363	3	0	theme	UV	712:713	arg1	light					715:719	a 8 kW UV light	705:719	a 8 kW UV light source (265 nm)	705:735	Methacrylated alginate solution was mixed with the required amount of surface-functionalized HAp nanoparticles in the presence of 0.05% Irgacure 2959 as a photoinitiator and was placed at the centre of a 8 kW UV light source (265 nm) to prepare photo-crosslinked bone paste.
32438363	3	1	theme	required	554:561	arg1	nanoparticles					600:612	surface-functionalized HAp nanoparticles	573:612	surface-functionalized HAp nanoparticles	573:612	Methacrylated alginate solution was mixed with the required amount of surface-functionalized HAp nanoparticles in the presence of 0.05% Irgacure 2959 as a photoinitiator and was placed at the centre of a 8 kW UV light source (265 nm) to prepare photo-crosslinked bone paste.
32438363	3	1	theme	required	554:561	arg1	amount					563:568	the required amount	550:568	the required amount of surface-functionalized HAp nanoparticles	550:612	Methacrylated alginate solution was mixed with the required amount of surface-functionalized HAp nanoparticles in the presence of 0.05% Irgacure 2959 as a photoinitiator and was placed at the centre of a 8 kW UV light source (265 nm) to prepare photo-crosslinked bone paste.
32438363	6	2	dep	100	1247:1249	arg1	to					1244:1245	to	1244:1245	to	1244:1245	The storage and loss moduli of all the prepared pastes increased non-linearly with time up to 100 s, demonstrating their pseudo plastic behaviour.
32438363	7	3	theme	release	1416:1422	arg1	curve					1424:1428	the release curve	1412:1428	the release curve	1412:1428	The rate of release of bone morphogenetic protein 2 (BMP-2) was significantly faster in the first few days, and the release curve gradually levelled off prior to slowing down up to 22 d. Mesenchymal stem cell adhesion studies revealed that cells could attach to the paste material and stretch over the surface of the material after 14 d of incubation.
32438363	2	4	from	glycol	362:367	arg1	presence					406:413	the presence	402:413	the presence of azobisisobutyronitrile benzene	402:447	Separately synthesized nano-hydroxyapatite (nano-HAp) powder was surface functionalized using mercaptopropionic acid and ethylene glycol methacrylate phosphate (EGMP) in the presence of azobisisobutyronitrile benzene as a free radical initiator in a nitrogen atmosphere.
32438363	9	5	theme	surface-functionalized	1831:1852	arg1	nano-HAp					1854:1861	surface-functionalized nano-HAp	1831:1861	surface-functionalized nano-HAp	1831:1861	Immunocytochemical analysis revealed that the addition of surface-functionalized nano-HAp and BMP-2 to alginate hydrogel enhanced the osteogenic potential of the prepared paste.
32438363	0	6	link	Photo-crosslinked	0:16	arg1	alginate					18:25	Photo-crosslinked alginate	0:25	Photo-crosslinked alginate	0:25	Photo-crosslinked alginate nano-hydroxyapatite paste for bone tissue engineering.
32438363	10	7	theme	bone	2086:2089	arg1	filler					2091:2096	a bone filler	2084:2096	a bone filler	2084:2096	The results indicate that the newly developed photo-crosslinked paste may be physically and biologically suitable for application as a bone filler.
32438363	3	8	theme	HAp	596:598	arg1	nanoparticles					600:612	surface-functionalized HAp nanoparticles	573:612	surface-functionalized HAp nanoparticles	573:612	Methacrylated alginate solution was mixed with the required amount of surface-functionalized HAp nanoparticles in the presence of 0.05% Irgacure 2959 as a photoinitiator and was placed at the centre of a 8 kW UV light source (265 nm) to prepare photo-crosslinked bone paste.
32438363	4	9	theme	nanopowder	880:889	arg1	purity					866:871	phase purity	860:871	phase purity of HAp nanopowder in the prepared paste	860:911	X-ray diffraction analysis indicated that surface functionalization did not alter phase purity of HAp nanopowder in the prepared paste.
32438363	3	10	theme	bone	766:769	arg1	paste					771:775	photo-crosslinked bone paste	748:775	photo-crosslinked bone paste	748:775	Methacrylated alginate solution was mixed with the required amount of surface-functionalized HAp nanoparticles in the presence of 0.05% Irgacure 2959 as a photoinitiator and was placed at the centre of a 8 kW UV light source (265 nm) to prepare photo-crosslinked bone paste.
32438363	3	11	theme	0.05	633:636	arg1	%					637:637	%	637:637	%	637:637	Methacrylated alginate solution was mixed with the required amount of surface-functionalized HAp nanoparticles in the presence of 0.05% Irgacure 2959 as a photoinitiator and was placed at the centre of a 8 kW UV light source (265 nm) to prepare photo-crosslinked bone paste.
32438363	5	12	theme	EGMP	1058:1061	arg1	stretching					1044:1053	C=-O stretching	1039:1053	C=-O stretching of EGMP	1039:1061	The graft polymerization of EGMP on the surface of HAp was confirmed by the presence of the 1732 cm-1 band, which belongs to C=-O stretching of EGMP, in addition to the characteristic peaks of nano-HAp and alginate in the composite paste.
32438363	2	13	theme	mercaptopropionic	326:342	arg1	acid					344:347	mercaptopropionic acid	326:347	mercaptopropionic acid	326:347	Separately synthesized nano-hydroxyapatite (nano-HAp) powder was surface functionalized using mercaptopropionic acid and ethylene glycol methacrylate phosphate (EGMP) in the presence of azobisisobutyronitrile benzene as a free radical initiator in a nitrogen atmosphere.
32438363	2	13	theme	mercaptopropionic	326:342	arg1	EGMP					393:396	EGMP	393:396	EGMP	393:396	Separately synthesized nano-hydroxyapatite (nano-HAp) powder was surface functionalized using mercaptopropionic acid and ethylene glycol methacrylate phosphate (EGMP) in the presence of azobisisobutyronitrile benzene as a free radical initiator in a nitrogen atmosphere.
32438363	2	14	theme	synthesized	243:253	arg1	powder					286:291	Separately synthesized nano-hydroxyapatite (nano-HAp) powder	232:291	Separately synthesized nano-hydroxyapatite (nano-HAp) powder	232:291	Separately synthesized nano-hydroxyapatite (nano-HAp) powder was surface functionalized using mercaptopropionic acid and ethylene glycol methacrylate phosphate (EGMP) in the presence of azobisisobutyronitrile benzene as a free radical initiator in a nitrogen atmosphere.
32438363	1	15	theme	hydroxide	221:229	arg1	presence					202:209	the presence	198:209	the presence of sodium hydroxide	198:229	In this study, methacrylation of alginate was carried out by reacting sodium alginate with methacrylic anhydride in the presence of sodium hydroxide.
32438363	6	16	theme	prepared	1192:1199	arg1	pastes					1201:1206	all the prepared pastes	1184:1206	all the prepared pastes	1184:1206	The storage and loss moduli of all the prepared pastes increased non-linearly with time up to 100 s, demonstrating their pseudo plastic behaviour.
32438363	2	17	dep	acid	344:347	arg1	phosphate					382:390	methacrylate phosphate	369:390	methacrylate phosphate	369:390	Separately synthesized nano-hydroxyapatite (nano-HAp) powder was surface functionalized using mercaptopropionic acid and ethylene glycol methacrylate phosphate (EGMP) in the presence of azobisisobutyronitrile benzene as a free radical initiator in a nitrogen atmosphere.
32438363	6	18	theme	pseudo	1274:1279	arg1	behaviour					1289:1297	their pseudo plastic behaviour	1268:1297	their pseudo plastic behaviour	1268:1297	The storage and loss moduli of all the prepared pastes increased non-linearly with time up to 100 s, demonstrating their pseudo plastic behaviour.
32438363	3	19	theme	Irgacure	639:646	arg1	2959					648:651	0.05% Irgacure 2959	633:651	0.05% Irgacure 2959	633:651	Methacrylated alginate solution was mixed with the required amount of surface-functionalized HAp nanoparticles in the presence of 0.05% Irgacure 2959 as a photoinitiator and was placed at the centre of a 8 kW UV light source (265 nm) to prepare photo-crosslinked bone paste.
32438363	8	20	theme	paste	1683:1687	arg1	materials					1689:1697	prepared paste materials	1674:1697	prepared paste materials	1674:1697	MTT assay showed that prepared paste materials were conducive to attachment and proliferation of mesenchymal stem cells.
32438363	5	21	theme	EGMP	942:945	arg1	polymerization					924:937	The graft polymerization	914:937	The graft polymerization of EGMP on the surface of HAp	914:967	The graft polymerization of EGMP on the surface of HAp was confirmed by the presence of the 1732 cm-1 band, which belongs to C=-O stretching of EGMP, in addition to the characteristic peaks of nano-HAp and alginate in the composite paste.
32438363	8	22	theme	cells	1766:1770	arg1	proliferation					1732:1744	proliferation	1732:1744	proliferation	1732:1744	MTT assay showed that prepared paste materials were conducive to attachment and proliferation of mesenchymal stem cells.
32438363	8	22	theme	cells	1766:1770	arg1	attachment					1717:1726	attachment	1717:1726	attachment	1717:1726	MTT assay showed that prepared paste materials were conducive to attachment and proliferation of mesenchymal stem cells.
32438363	7	23	attach	attach	1552:1557	arg1	stretch					1585:1591	stretch	1585:1591	stretch	1585:1591	The rate of release of bone morphogenetic protein 2 (BMP-2) was significantly faster in the first few days, and the release curve gradually levelled off prior to slowing down up to 22 d. Mesenchymal stem cell adhesion studies revealed that cells could attach to the paste material and stretch over the surface of the material after 14 d of incubation.
32438363	7	23	attach	attach	1552:1557	arg2	cells					1540:1544	cells	1540:1544	cells	1540:1544	The rate of release of bone morphogenetic protein 2 (BMP-2) was significantly faster in the first few days, and the release curve gradually levelled off prior to slowing down up to 22 d. Mesenchymal stem cell adhesion studies revealed that cells could attach to the paste material and stretch over the surface of the material after 14 d of incubation.
32438363	7	23	attach	attach	1552:1557	arg1	material					1572:1579	the paste material	1562:1579	the paste material	1562:1579	The rate of release of bone morphogenetic protein 2 (BMP-2) was significantly faster in the first few days, and the release curve gradually levelled off prior to slowing down up to 22 d. Mesenchymal stem cell adhesion studies revealed that cells could attach to the paste material and stretch over the surface of the material after 14 d of incubation.
32438363	7	24	theme	incubation	1640:1649	arg1	d					1635:1635	14 d	1632:1635	14 d of incubation	1632:1649	The rate of release of bone morphogenetic protein 2 (BMP-2) was significantly faster in the first few days, and the release curve gradually levelled off prior to slowing down up to 22 d. Mesenchymal stem cell adhesion studies revealed that cells could attach to the paste material and stretch over the surface of the material after 14 d of incubation.
32438363	3	25	theme	Methacrylated	503:515	arg1	photoinitiator					658:671	a photoinitiator	656:671	a photoinitiator	656:671	Methacrylated alginate solution was mixed with the required amount of surface-functionalized HAp nanoparticles in the presence of 0.05% Irgacure 2959 as a photoinitiator and was placed at the centre of a 8 kW UV light source (265 nm) to prepare photo-crosslinked bone paste.
32438363	3	25	theme	Methacrylated	503:515	arg1	solution					526:533	Methacrylated alginate solution	503:533	Methacrylated alginate solution	503:533	Methacrylated alginate solution was mixed with the required amount of surface-functionalized HAp nanoparticles in the presence of 0.05% Irgacure 2959 as a photoinitiator and was placed at the centre of a 8 kW UV light source (265 nm) to prepare photo-crosslinked bone paste.
32438363	10	26	link	photo-crosslinked	1997:2013	arg1	suitable					2056:2063	suitable	2056:2063	suitable	2056:2063	The results indicate that the newly developed photo-crosslinked paste may be physically and biologically suitable for application as a bone filler.
32438363	10	26	link	photo-crosslinked	1997:2013	arg1	paste					2015:2019	the newly developed photo-crosslinked paste	1977:2019	the newly developed photo-crosslinked paste	1977:2019	The results indicate that the newly developed photo-crosslinked paste may be physically and biologically suitable for application as a bone filler.
32438363	1	27	theme	sodium	152:157	arg1	alginate					159:166	sodium alginate	152:166	sodium alginate	152:166	In this study, methacrylation of alginate was carried out by reacting sodium alginate with methacrylic anhydride in the presence of sodium hydroxide.
32438363	2	28	from	acid	344:347	arg1	presence					406:413	the presence	402:413	the presence of azobisisobutyronitrile benzene	402:447	Separately synthesized nano-hydroxyapatite (nano-HAp) powder was surface functionalized using mercaptopropionic acid and ethylene glycol methacrylate phosphate (EGMP) in the presence of azobisisobutyronitrile benzene as a free radical initiator in a nitrogen atmosphere.
32438363	7	29	theme	adhesion	1509:1516	arg1	studies					1518:1524	Mesenchymal stem cell adhesion studies	1487:1524	Mesenchymal stem cell adhesion studies	1487:1524	The rate of release of bone morphogenetic protein 2 (BMP-2) was significantly faster in the first few days, and the release curve gradually levelled off prior to slowing down up to 22 d. Mesenchymal stem cell adhesion studies revealed that cells could attach to the paste material and stretch over the surface of the material after 14 d of incubation.
32438363	2	30	from	initiator	467:475	arg1	atmosphere					491:500	a nitrogen atmosphere	480:500	a nitrogen atmosphere	480:500	Separately synthesized nano-hydroxyapatite (nano-HAp) powder was surface functionalized using mercaptopropionic acid and ethylene glycol methacrylate phosphate (EGMP) in the presence of azobisisobutyronitrile benzene as a free radical initiator in a nitrogen atmosphere.
32438363	7	31	theme	stem	1499:1502	arg1	studies					1518:1524	Mesenchymal stem cell adhesion studies	1487:1524	Mesenchymal stem cell adhesion studies	1487:1524	The rate of release of bone morphogenetic protein 2 (BMP-2) was significantly faster in the first few days, and the release curve gradually levelled off prior to slowing down up to 22 d. Mesenchymal stem cell adhesion studies revealed that cells could attach to the paste material and stretch over the surface of the material after 14 d of incubation.
32438363	4	32	theme	X-ray	778:782	arg1	analysis					796:803	X-ray diffraction analysis	778:803	X-ray diffraction analysis	778:803	X-ray diffraction analysis indicated that surface functionalization did not alter phase purity of HAp nanopowder in the prepared paste.
32438363	10	33	theme	developed	1987:1995	arg1	suitable					2056:2063	suitable	2056:2063	suitable	2056:2063	The results indicate that the newly developed photo-crosslinked paste may be physically and biologically suitable for application as a bone filler.
32438363	10	33	theme	developed	1987:1995	arg1	paste					2015:2019	the newly developed photo-crosslinked paste	1977:2019	the newly developed photo-crosslinked paste	1977:2019	The results indicate that the newly developed photo-crosslinked paste may be physically and biologically suitable for application as a bone filler.
32438363	2	34	theme	free	454:457	arg1	radical					459:465	a free radical	452:465	a free radical initiator in a nitrogen atmosphere	452:500	Separately synthesized nano-hydroxyapatite (nano-HAp) powder was surface functionalized using mercaptopropionic acid and ethylene glycol methacrylate phosphate (EGMP) in the presence of azobisisobutyronitrile benzene as a free radical initiator in a nitrogen atmosphere.
32438363	2	35	theme	benzene	441:447	arg1	presence					406:413	the presence	402:413	the presence of azobisisobutyronitrile benzene	402:447	Separately synthesized nano-hydroxyapatite (nano-HAp) powder was surface functionalized using mercaptopropionic acid and ethylene glycol methacrylate phosphate (EGMP) in the presence of azobisisobutyronitrile benzene as a free radical initiator in a nitrogen atmosphere.
32438363	5	36	theme	characteristic	1083:1096	arg1	peaks					1098:1102	the characteristic peaks	1079:1102	the characteristic peaks of nano-HAp and alginate in the composite paste	1079:1150	The graft polymerization of EGMP on the surface of HAp was confirmed by the presence of the 1732 cm-1 band, which belongs to C=-O stretching of EGMP, in addition to the characteristic peaks of nano-HAp and alginate in the composite paste.
32438363	8	37	theme	stem	1761:1764	arg1	cells					1766:1770	mesenchymal stem cells	1749:1770	mesenchymal stem cells	1749:1770	MTT assay showed that prepared paste materials were conducive to attachment and proliferation of mesenchymal stem cells.
32438363	2	38	theme	nitrogen	482:489	arg1	atmosphere					491:500	a nitrogen atmosphere	480:500	a nitrogen atmosphere	480:500	Separately synthesized nano-hydroxyapatite (nano-HAp) powder was surface functionalized using mercaptopropionic acid and ethylene glycol methacrylate phosphate (EGMP) in the presence of azobisisobutyronitrile benzene as a free radical initiator in a nitrogen atmosphere.
32438363	0	39	theme	Photo-crosslinked	0:16	arg1	alginate					18:25	Photo-crosslinked alginate	0:25	Photo-crosslinked alginate	0:25	Photo-crosslinked alginate nano-hydroxyapatite paste for bone tissue engineering.
32438363	2	40	theme	radical	459:465	arg1	initiator					467:475	a free radical initiator	452:475	a free radical initiator in a nitrogen atmosphere	452:500	Separately synthesized nano-hydroxyapatite (nano-HAp) powder was surface functionalized using mercaptopropionic acid and ethylene glycol methacrylate phosphate (EGMP) in the presence of azobisisobutyronitrile benzene as a free radical initiator in a nitrogen atmosphere.
32438363	3	41	link	photo-crosslinked	748:764	arg1	paste					771:775	photo-crosslinked bone paste	748:775	photo-crosslinked bone paste	748:775	Methacrylated alginate solution was mixed with the required amount of surface-functionalized HAp nanoparticles in the presence of 0.05% Irgacure 2959 as a photoinitiator and was placed at the centre of a 8 kW UV light source (265 nm) to prepare photo-crosslinked bone paste.
32438363	1	42	theme	alginate	115:122	arg1	methacrylation					97:110	methacrylation	97:110	methacrylation of alginate	97:122	In this study, methacrylation of alginate was carried out by reacting sodium alginate with methacrylic anhydride in the presence of sodium hydroxide.
32438363	5	43	dep	peaks	1098:1102	arg1	addition					1067:1074	addition	1067:1074	addition	1067:1074	The graft polymerization of EGMP on the surface of HAp was confirmed by the presence of the 1732 cm-1 band, which belongs to C=-O stretching of EGMP, in addition to the characteristic peaks of nano-HAp and alginate in the composite paste.
32438363	4	44	theme	phase	860:864	arg1	purity					866:871	phase purity	860:871	phase purity of HAp nanopowder in the prepared paste	860:911	X-ray diffraction analysis indicated that surface functionalization did not alter phase purity of HAp nanopowder in the prepared paste.
32438363	0	45	theme	tissue	62:67	arg1	engineering					69:79	bone tissue engineering	57:79	bone tissue engineering	57:79	Photo-crosslinked alginate nano-hydroxyapatite paste for bone tissue engineering.
32438363	5	46	theme	composite	1136:1144	arg1	paste					1146:1150	the composite paste	1132:1150	the composite paste	1132:1150	The graft polymerization of EGMP on the surface of HAp was confirmed by the presence of the 1732 cm-1 band, which belongs to C=-O stretching of EGMP, in addition to the characteristic peaks of nano-HAp and alginate in the composite paste.
32438363	9	47	theme	Immunocytochemical	1773:1790	arg1	analysis					1792:1799	Immunocytochemical analysis	1773:1799	Immunocytochemical analysis	1773:1799	Immunocytochemical analysis revealed that the addition of surface-functionalized nano-HAp and BMP-2 to alginate hydrogel enhanced the osteogenic potential of the prepared paste.
32438363	3	48	theme	kW	709:710	arg1	nm					733:734	265 nm	729:734	265 nm	729:734	Methacrylated alginate solution was mixed with the required amount of surface-functionalized HAp nanoparticles in the presence of 0.05% Irgacure 2959 as a photoinitiator and was placed at the centre of a 8 kW UV light source (265 nm) to prepare photo-crosslinked bone paste.
32438363	3	48	theme	kW	709:710	arg1	light					715:719	a 8 kW UV light	705:719	a 8 kW UV light source (265 nm)	705:735	Methacrylated alginate solution was mixed with the required amount of surface-functionalized HAp nanoparticles in the presence of 0.05% Irgacure 2959 as a photoinitiator and was placed at the centre of a 8 kW UV light source (265 nm) to prepare photo-crosslinked bone paste.
32438363	2	49	theme	methacrylate	369:380	arg1	phosphate					382:390	methacrylate phosphate	369:390	methacrylate phosphate	369:390	Separately synthesized nano-hydroxyapatite (nano-HAp) powder was surface functionalized using mercaptopropionic acid and ethylene glycol methacrylate phosphate (EGMP) in the presence of azobisisobutyronitrile benzene as a free radical initiator in a nitrogen atmosphere.
32438363	5	50	theme	cm-1	1011:1014	arg1	band					1016:1019	the 1732 cm-1 band	1002:1019	the 1732 cm-1 band	1002:1019	The graft polymerization of EGMP on the surface of HAp was confirmed by the presence of the 1732 cm-1 band, which belongs to C=-O stretching of EGMP, in addition to the characteristic peaks of nano-HAp and alginate in the composite paste.
32438363	9	51	theme	paste	1944:1948	arg1	potential					1918:1926	the osteogenic potential	1903:1926	the osteogenic potential of the prepared paste	1903:1948	Immunocytochemical analysis revealed that the addition of surface-functionalized nano-HAp and BMP-2 to alginate hydrogel enhanced the osteogenic potential of the prepared paste.
32438363	6	52	dep	time	1236:1239	arg1	s					1251:1251	s	1251:1251	s	1251:1251	The storage and loss moduli of all the prepared pastes increased non-linearly with time up to 100 s, demonstrating their pseudo plastic behaviour.
32438363	3	53	theme	light	715:719	arg1	centre					695:700	the centre	691:700	the centre of a 8 kW UV light source (265 nm)	691:735	Methacrylated alginate solution was mixed with the required amount of surface-functionalized HAp nanoparticles in the presence of 0.05% Irgacure 2959 as a photoinitiator and was placed at the centre of a 8 kW UV light source (265 nm) to prepare photo-crosslinked bone paste.
32438363	3	54	theme	nanoparticles	600:612	arg1	nanoparticles					600:612	surface-functionalized HAp nanoparticles	573:612	surface-functionalized HAp nanoparticles	573:612	Methacrylated alginate solution was mixed with the required amount of surface-functionalized HAp nanoparticles in the presence of 0.05% Irgacure 2959 as a photoinitiator and was placed at the centre of a 8 kW UV light source (265 nm) to prepare photo-crosslinked bone paste.
32438363	3	54	theme	nanoparticles	600:612	arg1	amount					563:568	the required amount	550:568	the required amount of surface-functionalized HAp nanoparticles	550:612	Methacrylated alginate solution was mixed with the required amount of surface-functionalized HAp nanoparticles in the presence of 0.05% Irgacure 2959 as a photoinitiator and was placed at the centre of a 8 kW UV light source (265 nm) to prepare photo-crosslinked bone paste.
32438363	4	55	theme	prepared	898:905	arg1	paste					907:911	the prepared paste	894:911	the prepared paste	894:911	X-ray diffraction analysis indicated that surface functionalization did not alter phase purity of HAp nanopowder in the prepared paste.
32438363	7	56	theme	first	1392:1396	arg1	days					1402:1405	the first few days	1388:1405	the first few days	1388:1405	The rate of release of bone morphogenetic protein 2 (BMP-2) was significantly faster in the first few days, and the release curve gradually levelled off prior to slowing down up to 22 d. Mesenchymal stem cell adhesion studies revealed that cells could attach to the paste material and stretch over the surface of the material after 14 d of incubation.
32438363	9	57	theme	nano-HAp	1854:1861	arg1	addition					1819:1826	the addition	1815:1826	the addition of surface-functionalized nano-HAp and BMP-2 to alginate hydrogel	1815:1892	Immunocytochemical analysis revealed that the addition of surface-functionalized nano-HAp and BMP-2 to alginate hydrogel enhanced the osteogenic potential of the prepared paste.
32438363	3	58	theme	surface-functionalized	573:594	arg1	nanoparticles					600:612	surface-functionalized HAp nanoparticles	573:612	surface-functionalized HAp nanoparticles	573:612	Methacrylated alginate solution was mixed with the required amount of surface-functionalized HAp nanoparticles in the presence of 0.05% Irgacure 2959 as a photoinitiator and was placed at the centre of a 8 kW UV light source (265 nm) to prepare photo-crosslinked bone paste.
32438363	1	59	theme	methacrylic	173:183	arg1	anhydride					185:193	methacrylic anhydride	173:193	methacrylic anhydride in the presence of sodium hydroxide	173:229	In this study, methacrylation of alginate was carried out by reacting sodium alginate with methacrylic anhydride in the presence of sodium hydroxide.
32438363	6	60	dep	s	1251:1251	arg1	100					1247:1249	100	1247:1249	100	1247:1249	The storage and loss moduli of all the prepared pastes increased non-linearly with time up to 100 s, demonstrating their pseudo plastic behaviour.
32438363	7	61	theme	few	1398:1400	arg1	days					1402:1405	the first few days	1388:1405	the first few days	1388:1405	The rate of release of bone morphogenetic protein 2 (BMP-2) was significantly faster in the first few days, and the release curve gradually levelled off prior to slowing down up to 22 d. Mesenchymal stem cell adhesion studies revealed that cells could attach to the paste material and stretch over the surface of the material after 14 d of incubation.
32438363	7	62	dep	d.	1484:1485	arg1	revealed					1526:1533	revealed	1526:1533	revealed that cells could attach to the paste material and stretch over the surface of the material after 14 d of incubation	1526:1649	The rate of release of bone morphogenetic protein 2 (BMP-2) was significantly faster in the first few days, and the release curve gradually levelled off prior to slowing down up to 22 d. Mesenchymal stem cell adhesion studies revealed that cells could attach to the paste material and stretch over the surface of the material after 14 d of incubation.
32438363	9	63	theme	BMP-2	1867:1871	arg1	addition					1819:1826	the addition	1815:1826	the addition of surface-functionalized nano-HAp and BMP-2 to alginate hydrogel	1815:1892	Immunocytochemical analysis revealed that the addition of surface-functionalized nano-HAp and BMP-2 to alginate hydrogel enhanced the osteogenic potential of the prepared paste.
32438363	2	64	theme	ethylene	353:360	arg1	glycol					362:367	ethylene glycol	353:367	ethylene glycol	353:367	Separately synthesized nano-hydroxyapatite (nano-HAp) powder was surface functionalized using mercaptopropionic acid and ethylene glycol methacrylate phosphate (EGMP) in the presence of azobisisobutyronitrile benzene as a free radical initiator in a nitrogen atmosphere.
32438363	7	65	theme	paste	1566:1570	arg1	material					1572:1579	the paste material	1562:1579	the paste material	1562:1579	The rate of release of bone morphogenetic protein 2 (BMP-2) was significantly faster in the first few days, and the release curve gradually levelled off prior to slowing down up to 22 d. Mesenchymal stem cell adhesion studies revealed that cells could attach to the paste material and stretch over the surface of the material after 14 d of incubation.
32438363	4	66	theme	HAp	876:878	arg1	nanopowder					880:889	HAp nanopowder	876:889	HAp nanopowder	876:889	X-ray diffraction analysis indicated that surface functionalization did not alter phase purity of HAp nanopowder in the prepared paste.
32438363	9	67	theme	alginate	1876:1883	arg1	hydrogel					1885:1892	alginate hydrogel	1876:1892	alginate hydrogel	1876:1892	Immunocytochemical analysis revealed that the addition of surface-functionalized nano-HAp and BMP-2 to alginate hydrogel enhanced the osteogenic potential of the prepared paste.
32438363	7	68	theme	morphogenetic	1328:1340	arg1	protein					1342:1348	bone morphogenetic protein 2	1323:1350	bone morphogenetic protein 2 (BMP-2)	1323:1358	The rate of release of bone morphogenetic protein 2 (BMP-2) was significantly faster in the first few days, and the release curve gradually levelled off prior to slowing down up to 22 d. Mesenchymal stem cell adhesion studies revealed that cells could attach to the paste material and stretch over the surface of the material after 14 d of incubation.
32438363	7	68	theme	morphogenetic	1328:1340	arg1	BMP-2					1353:1357	BMP-2	1353:1357	BMP-2	1353:1357	The rate of release of bone morphogenetic protein 2 (BMP-2) was significantly faster in the first few days, and the release curve gradually levelled off prior to slowing down up to 22 d. Mesenchymal stem cell adhesion studies revealed that cells could attach to the paste material and stretch over the surface of the material after 14 d of incubation.
32438363	3	69	theme	photo-crosslinked	748:764	arg1	paste					771:775	photo-crosslinked bone paste	748:775	photo-crosslinked bone paste	748:775	Methacrylated alginate solution was mixed with the required amount of surface-functionalized HAp nanoparticles in the presence of 0.05% Irgacure 2959 as a photoinitiator and was placed at the centre of a 8 kW UV light source (265 nm) to prepare photo-crosslinked bone paste.
32438363	3	70	theme	2959	648:651	arg1	presence					621:628	the presence	617:628	the presence of 0.05% Irgacure 2959	617:651	Methacrylated alginate solution was mixed with the required amount of surface-functionalized HAp nanoparticles in the presence of 0.05% Irgacure 2959 as a photoinitiator and was placed at the centre of a 8 kW UV light source (265 nm) to prepare photo-crosslinked bone paste.
32438363	8	71	theme	MTT	1652:1654	arg1	assay					1656:1660	MTT assay	1652:1660	MTT assay	1652:1660	MTT assay showed that prepared paste materials were conducive to attachment and proliferation of mesenchymal stem cells.
32438363	1	72	theme	sodium	214:219	arg1	hydroxide					221:229	sodium hydroxide	214:229	sodium hydroxide	214:229	In this study, methacrylation of alginate was carried out by reacting sodium alginate with methacrylic anhydride in the presence of sodium hydroxide.
32438363	6	73	theme	pastes	1201:1206	arg1	loss					1169:1172	loss	1169:1172	loss	1169:1172	The storage and loss moduli of all the prepared pastes increased non-linearly with time up to 100 s, demonstrating their pseudo plastic behaviour.
32438363	6	73	theme	pastes	1201:1206	arg1	storage					1157:1163	storage	1157:1163	storage	1157:1163	The storage and loss moduli of all the prepared pastes increased non-linearly with time up to 100 s, demonstrating their pseudo plastic behaviour.
32438363	9	74	theme	osteogenic	1907:1916	arg1	potential					1918:1926	the osteogenic potential	1903:1926	the osteogenic potential of the prepared paste	1903:1948	Immunocytochemical analysis revealed that the addition of surface-functionalized nano-HAp and BMP-2 to alginate hydrogel enhanced the osteogenic potential of the prepared paste.
32438363	6	75	dep	storage	1157:1163	arg1	The					1153:1155	The	1153:1155	The	1153:1155	The storage and loss moduli of all the prepared pastes increased non-linearly with time up to 100 s, demonstrating their pseudo plastic behaviour.
32438363	6	75	dep	storage	1157:1163	arg1	moduli					1174:1179	moduli	1174:1179	moduli	1174:1179	The storage and loss moduli of all the prepared pastes increased non-linearly with time up to 100 s, demonstrating their pseudo plastic behaviour.
32438363	6	76	theme	plastic	1281:1287	arg1	behaviour					1289:1297	their pseudo plastic behaviour	1268:1297	their pseudo plastic behaviour	1268:1297	The storage and loss moduli of all the prepared pastes increased non-linearly with time up to 100 s, demonstrating their pseudo plastic behaviour.
32438363	3	77	theme	%	637:637	arg1	2959					648:651	0.05% Irgacure 2959	633:651	0.05% Irgacure 2959	633:651	Methacrylated alginate solution was mixed with the required amount of surface-functionalized HAp nanoparticles in the presence of 0.05% Irgacure 2959 as a photoinitiator and was placed at the centre of a 8 kW UV light source (265 nm) to prepare photo-crosslinked bone paste.
32438363	5	78	from	peaks	1098:1102	arg1	paste					1146:1150	the composite paste	1132:1150	the composite paste	1132:1150	The graft polymerization of EGMP on the surface of HAp was confirmed by the presence of the 1732 cm-1 band, which belongs to C=-O stretching of EGMP, in addition to the characteristic peaks of nano-HAp and alginate in the composite paste.
32438363	7	79	theme	protein	1342:1348	arg1	release					1312:1318	release	1312:1318	release of bone morphogenetic protein 2 (BMP-2)	1312:1358	The rate of release of bone morphogenetic protein 2 (BMP-2) was significantly faster in the first few days, and the release curve gradually levelled off prior to slowing down up to 22 d. Mesenchymal stem cell adhesion studies revealed that cells could attach to the paste material and stretch over the surface of the material after 14 d of incubation.
32438363	5	80	theme	C=-O	1039:1042	arg1	stretching					1044:1053	C=-O stretching	1039:1053	C=-O stretching of EGMP	1039:1061	The graft polymerization of EGMP on the surface of HAp was confirmed by the presence of the 1732 cm-1 band, which belongs to C=-O stretching of EGMP, in addition to the characteristic peaks of nano-HAp and alginate in the composite paste.
32438363	8	81	theme	prepared	1674:1681	arg1	materials					1689:1697	prepared paste materials	1674:1697	prepared paste materials	1674:1697	MTT assay showed that prepared paste materials were conducive to attachment and proliferation of mesenchymal stem cells.
32438363	7	82	theme	bone	1323:1326	arg1	protein					1342:1348	bone morphogenetic protein 2	1323:1350	bone morphogenetic protein 2 (BMP-2)	1323:1358	The rate of release of bone morphogenetic protein 2 (BMP-2) was significantly faster in the first few days, and the release curve gradually levelled off prior to slowing down up to 22 d. Mesenchymal stem cell adhesion studies revealed that cells could attach to the paste material and stretch over the surface of the material after 14 d of incubation.
32438363	7	82	theme	bone	1323:1326	arg1	BMP-2					1353:1357	BMP-2	1353:1357	BMP-2	1353:1357	The rate of release of bone morphogenetic protein 2 (BMP-2) was significantly faster in the first few days, and the release curve gradually levelled off prior to slowing down up to 22 d. Mesenchymal stem cell adhesion studies revealed that cells could attach to the paste material and stretch over the surface of the material after 14 d of incubation.
32438363	9	83	theme	prepared	1935:1942	arg1	paste					1944:1948	the prepared paste	1931:1948	the prepared paste	1931:1948	Immunocytochemical analysis revealed that the addition of surface-functionalized nano-HAp and BMP-2 to alginate hydrogel enhanced the osteogenic potential of the prepared paste.
32438363	2	84	theme	nano-HAp	276:283	arg1	powder					286:291	Separately synthesized nano-hydroxyapatite (nano-HAp) powder	232:291	Separately synthesized nano-hydroxyapatite (nano-HAp) powder	232:291	Separately synthesized nano-hydroxyapatite (nano-HAp) powder was surface functionalized using mercaptopropionic acid and ethylene glycol methacrylate phosphate (EGMP) in the presence of azobisisobutyronitrile benzene as a free radical initiator in a nitrogen atmosphere.
32438363	7	85	theme	cell	1504:1507	arg1	studies					1518:1524	Mesenchymal stem cell adhesion studies	1487:1524	Mesenchymal stem cell adhesion studies	1487:1524	The rate of release of bone morphogenetic protein 2 (BMP-2) was significantly faster in the first few days, and the release curve gradually levelled off prior to slowing down up to 22 d. Mesenchymal stem cell adhesion studies revealed that cells could attach to the paste material and stretch over the surface of the material after 14 d of incubation.
32438363	7	86	theme	release	1312:1318	arg1	faster					1378:1383	faster	1378:1383	faster	1378:1383	The rate of release of bone morphogenetic protein 2 (BMP-2) was significantly faster in the first few days, and the release curve gradually levelled off prior to slowing down up to 22 d. Mesenchymal stem cell adhesion studies revealed that cells could attach to the paste material and stretch over the surface of the material after 14 d of incubation.
32438363	7	86	theme	release	1312:1318	arg1	rate					1304:1307	The rate	1300:1307	The rate of release of bone morphogenetic protein 2 (BMP-2)	1300:1358	The rate of release of bone morphogenetic protein 2 (BMP-2) was significantly faster in the first few days, and the release curve gradually levelled off prior to slowing down up to 22 d. Mesenchymal stem cell adhesion studies revealed that cells could attach to the paste material and stretch over the surface of the material after 14 d of incubation.
32438363	5	87	theme	nano-HAp	1107:1114	arg1	peaks					1098:1102	the characteristic peaks	1079:1102	the characteristic peaks of nano-HAp and alginate in the composite paste	1079:1150	The graft polymerization of EGMP on the surface of HAp was confirmed by the presence of the 1732 cm-1 band, which belongs to C=-O stretching of EGMP, in addition to the characteristic peaks of nano-HAp and alginate in the composite paste.
32438363	1	88	from	anhydride	185:193	arg1	presence					202:209	the presence	198:209	the presence of sodium hydroxide	198:229	In this study, methacrylation of alginate was carried out by reacting sodium alginate with methacrylic anhydride in the presence of sodium hydroxide.
32438363	5	89	theme	graft	918:922	arg1	polymerization					924:937	The graft polymerization	914:937	The graft polymerization of EGMP on the surface of HAp	914:967	The graft polymerization of EGMP on the surface of HAp was confirmed by the presence of the 1732 cm-1 band, which belongs to C=-O stretching of EGMP, in addition to the characteristic peaks of nano-HAp and alginate in the composite paste.
32438363	7	90	theme	Mesenchymal	1487:1497	arg1	studies					1518:1524	Mesenchymal stem cell adhesion studies	1487:1524	Mesenchymal stem cell adhesion studies	1487:1524	The rate of release of bone morphogenetic protein 2 (BMP-2) was significantly faster in the first few days, and the release curve gradually levelled off prior to slowing down up to 22 d. Mesenchymal stem cell adhesion studies revealed that cells could attach to the paste material and stretch over the surface of the material after 14 d of incubation.
32438363	2	91	dep	functionalized	305:318	arg1	surface					297:303	surface	297:303	surface	297:303	Separately synthesized nano-hydroxyapatite (nano-HAp) powder was surface functionalized using mercaptopropionic acid and ethylene glycol methacrylate phosphate (EGMP) in the presence of azobisisobutyronitrile benzene as a free radical initiator in a nitrogen atmosphere.
32438363	10	92	theme	photo-crosslinked	1997:2013	arg1	suitable					2056:2063	suitable	2056:2063	suitable	2056:2063	The results indicate that the newly developed photo-crosslinked paste may be physically and biologically suitable for application as a bone filler.
32438363	10	92	theme	photo-crosslinked	1997:2013	arg1	paste					2015:2019	the newly developed photo-crosslinked paste	1977:2019	the newly developed photo-crosslinked paste	1977:2019	The results indicate that the newly developed photo-crosslinked paste may be physically and biologically suitable for application as a bone filler.
32438363	5	93	theme	alginate	1120:1127	arg1	peaks					1098:1102	the characteristic peaks	1079:1102	the characteristic peaks of nano-HAp and alginate in the composite paste	1079:1150	The graft polymerization of EGMP on the surface of HAp was confirmed by the presence of the 1732 cm-1 band, which belongs to C=-O stretching of EGMP, in addition to the characteristic peaks of nano-HAp and alginate in the composite paste.
32438363	5	94	from	polymerization	924:937	arg1	surface					954:960	the surface	950:960	the surface of HAp	950:967	The graft polymerization of EGMP on the surface of HAp was confirmed by the presence of the 1732 cm-1 band, which belongs to C=-O stretching of EGMP, in addition to the characteristic peaks of nano-HAp and alginate in the composite paste.
32438363	7	95	theme	material	1617:1624	arg1	surface					1602:1608	the surface	1598:1608	the surface of the material after 14 d of incubation	1598:1649	The rate of release of bone morphogenetic protein 2 (BMP-2) was significantly faster in the first few days, and the release curve gradually levelled off prior to slowing down up to 22 d. Mesenchymal stem cell adhesion studies revealed that cells could attach to the paste material and stretch over the surface of the material after 14 d of incubation.
32438363	4	96	theme	diffraction	784:794	arg1	analysis					796:803	X-ray diffraction analysis	778:803	X-ray diffraction analysis	778:803	X-ray diffraction analysis indicated that surface functionalization did not alter phase purity of HAp nanopowder in the prepared paste.
32438363	2	97	theme	azobisisobutyronitrile	418:439	arg1	benzene					441:447	azobisisobutyronitrile benzene	418:447	azobisisobutyronitrile benzene	418:447	Separately synthesized nano-hydroxyapatite (nano-HAp) powder was surface functionalized using mercaptopropionic acid and ethylene glycol methacrylate phosphate (EGMP) in the presence of azobisisobutyronitrile benzene as a free radical initiator in a nitrogen atmosphere.
32438363	4	98	from	purity	866:871	arg1	paste					907:911	the prepared paste	894:911	the prepared paste	894:911	X-ray diffraction analysis indicated that surface functionalization did not alter phase purity of HAp nanopowder in the prepared paste.
32438363	8	99	theme	mesenchymal	1749:1759	arg1	cells					1766:1770	mesenchymal stem cells	1749:1770	mesenchymal stem cells	1749:1770	MTT assay showed that prepared paste materials were conducive to attachment and proliferation of mesenchymal stem cells.
32438363	3	100	dep	light	715:719	arg1	source					721:726	source	721:726	a 8 kW UV light source (265 nm)	705:735	Methacrylated alginate solution was mixed with the required amount of surface-functionalized HAp nanoparticles in the presence of 0.05% Irgacure 2959 as a photoinitiator and was placed at the centre of a 8 kW UV light source (265 nm) to prepare photo-crosslinked bone paste.
32438363	5	101	from	EGMP	942:945	arg1	surface					954:960	the surface	950:960	the surface of HAp	950:967	The graft polymerization of EGMP on the surface of HAp was confirmed by the presence of the 1732 cm-1 band, which belongs to C=-O stretching of EGMP, in addition to the characteristic peaks of nano-HAp and alginate in the composite paste.
32438363	5	102	theme	HAp	965:967	arg1	surface					954:960	the surface	950:960	the surface of HAp	950:967	The graft polymerization of EGMP on the surface of HAp was confirmed by the presence of the 1732 cm-1 band, which belongs to C=-O stretching of EGMP, in addition to the characteristic peaks of nano-HAp and alginate in the composite paste.
32438363	2	103	theme	nano-hydroxyapatite	255:273	arg1	powder					286:291	Separately synthesized nano-hydroxyapatite (nano-HAp) powder	232:291	Separately synthesized nano-hydroxyapatite (nano-HAp) powder	232:291	Separately synthesized nano-hydroxyapatite (nano-HAp) powder was surface functionalized using mercaptopropionic acid and ethylene glycol methacrylate phosphate (EGMP) in the presence of azobisisobutyronitrile benzene as a free radical initiator in a nitrogen atmosphere.
32438363	3	104	theme	alginate	517:524	arg1	photoinitiator					658:671	a photoinitiator	656:671	a photoinitiator	656:671	Methacrylated alginate solution was mixed with the required amount of surface-functionalized HAp nanoparticles in the presence of 0.05% Irgacure 2959 as a photoinitiator and was placed at the centre of a 8 kW UV light source (265 nm) to prepare photo-crosslinked bone paste.
32438363	3	104	theme	alginate	517:524	arg1	solution					526:533	Methacrylated alginate solution	503:533	Methacrylated alginate solution	503:533	Methacrylated alginate solution was mixed with the required amount of surface-functionalized HAp nanoparticles in the presence of 0.05% Irgacure 2959 as a photoinitiator and was placed at the centre of a 8 kW UV light source (265 nm) to prepare photo-crosslinked bone paste.
32438363	0	105	theme	bone	57:60	arg1	engineering					69:79	bone tissue engineering	57:79	bone tissue engineering	57:79	Photo-crosslinked alginate nano-hydroxyapatite paste for bone tissue engineering.
32438363	5	106	theme	band	1016:1019	arg1	presence					990:997	the presence	986:997	the presence of the 1732 cm-1 band, which belongs to C=-O stretching of EGMP, in addition to the characteristic peaks of nano-HAp and alginate in the composite paste	986:1150	The graft polymerization of EGMP on the surface of HAp was confirmed by the presence of the 1732 cm-1 band, which belongs to C=-O stretching of EGMP, in addition to the characteristic peaks of nano-HAp and alginate in the composite paste.
32438363	7	107	theme	down	1470:1473	arg1	slowing					1462:1468	slowing	1462:1468	slowing down up to 22 d. Mesenchymal stem cell adhesion studies revealed that cells could attach to the paste material and stretch over the surface of the material after 14 d of incubation	1462:1649	The rate of release of bone morphogenetic protein 2 (BMP-2) was significantly faster in the first few days, and the release curve gradually levelled off prior to slowing down up to 22 d. Mesenchymal stem cell adhesion studies revealed that cells could attach to the paste material and stretch over the surface of the material after 14 d of incubation.
32438363	3	108	theme	8	707:707	arg1	kW					709:710	kW	709:710	kW	709:710	Methacrylated alginate solution was mixed with the required amount of surface-functionalized HAp nanoparticles in the presence of 0.05% Irgacure 2959 as a photoinitiator and was placed at the centre of a 8 kW UV light source (265 nm) to prepare photo-crosslinked bone paste.
32438363	5	109	from	surface	954:960	arg1	polymerization					924:937	The graft polymerization	914:937	The graft polymerization of EGMP on the surface of HAp	914:967	The graft polymerization of EGMP on the surface of HAp was confirmed by the presence of the 1732 cm-1 band, which belongs to C=-O stretching of EGMP, in addition to the characteristic peaks of nano-HAp and alginate in the composite paste.
32438363	5	110	theme	1732	1006:1009	arg1	cm-1					1011:1014	cm-1	1011:1014	cm-1	1011:1014	The graft polymerization of EGMP on the surface of HAp was confirmed by the presence of the 1732 cm-1 band, which belongs to C=-O stretching of EGMP, in addition to the characteristic peaks of nano-HAp and alginate in the composite paste.
32438363	4	111	theme	surface	820:826	arg1	functionalization					828:844	surface functionalization	820:844	surface functionalization	820:844	X-ray diffraction analysis indicated that surface functionalization did not alter phase purity of HAp nanopowder in the prepared paste.
34896162	2	0	theme	dextran	355:361	arg1	DSS					379:381	DSS	379:381	DSS	379:381	In this study, we examined whether colitis induced by dextran sulfate sodium (DSS) increased the susceptibility to chronic unpredictable mild stress (CUMS) in C57BL/6J mice with resilience to CUMS.
34896162	2	0	theme	dextran	355:361	arg1	sodium					371:376	dextran sulfate sodium	355:376	dextran sulfate sodium (DSS)	355:382	In this study, we examined whether colitis induced by dextran sulfate sodium (DSS) increased the susceptibility to chronic unpredictable mild stress (CUMS) in C57BL/6J mice with resilience to CUMS.
34896162	6	1	theme	immobility	1371:1380	arg1	times					1382:1386	the immobility times	1367:1386	the immobility times in the FS test	1367:1401	Exposure of mice with DSS-induced colitis to CUMS (DSS + CUMS) significantly increased the immobility times in the FS test.
34896162	1	2	theme	detailed	262:269	arg1	mechanisms					271:280	the detailed mechanisms	258:280	the detailed mechanisms	258:280	AIMS In patients with colitis, the high comorbidity of depressive disorders is well-known, but the detailed mechanisms remain unresolved.
34896162	5	3	dep	Alistipes	1224:1232	arg1	spp.					1234:1237	Alistipes spp.	1224:1237	Alistipes spp.	1224:1237	The DSS administration to naïve mice induced colitis without depressive-like behavior, and at 18 days after termination of the DSS administration, the colitis had recovered to control levels, while altered diversity and composition of bacterial genera such as Bacteroides spp., Alistipes spp., etc., were found in the gut microbiota.
34896162	5	4	theme	altered	1144:1150	arg1	diversity					1152:1160	altered diversity	1144:1160	altered diversity	1144:1160	The DSS administration to naïve mice induced colitis without depressive-like behavior, and at 18 days after termination of the DSS administration, the colitis had recovered to control levels, while altered diversity and composition of bacterial genera such as Bacteroides spp., Alistipes spp., etc., were found in the gut microbiota.
34896162	6	5	theme	mice	1292:1295	arg1	Exposure					1280:1287	Exposure	1280:1287	Exposure of mice with DSS-induced colitis to CUMS (DSS + CUMS)	1280:1341	Exposure of mice with DSS-induced colitis to CUMS (DSS + CUMS) significantly increased the immobility times in the FS test.
34896162	3	6	theme	experimental	522:533	arg1	colitis					535:541	experimental colitis	522:541	experimental colitis	522:541	MAIN METHODS To induce experimental colitis and depressive-like behaviors, male 7-weeks old C57BL/6J mice were administered ad libitum 1% DSS solution for 11 days, and subjected to various mild stressors in a chronic, inevitable and unpredictable way according to a random schedule for 21 days, respectively.
34896162	3	7	theme	male	574:577	arg1	mice					600:603	male 7-weeks old C57BL/6J mice	574:603	male 7-weeks old C57BL/6J mice	574:603	MAIN METHODS To induce experimental colitis and depressive-like behaviors, male 7-weeks old C57BL/6J mice were administered ad libitum 1% DSS solution for 11 days, and subjected to various mild stressors in a chronic, inevitable and unpredictable way according to a random schedule for 21 days, respectively.
34896162	8	8	theme	psychological	1642:1654	arg1	stress					1656:1661	psychological stress	1642:1661	psychological stress	1642:1661	SIGNIFICANCE These findings indicate that mice with colitis exhibit increased susceptibility to psychological stress, resulting in induction of depressive-like behavior, and this might be due, at least in part, to altered characteristics of the gut microbiota.
34896162	1	9	theme	high	198:201	arg1	colitis					185:191	colitis	185:191	colitis	185:191	AIMS In patients with colitis, the high comorbidity of depressive disorders is well-known, but the detailed mechanisms remain unresolved.
34896162	1	9	theme	high	198:201	arg1	comorbidity					203:213	the high comorbidity	194:213	the high comorbidity of depressive disorders	194:237	AIMS In patients with colitis, the high comorbidity of depressive disorders is well-known, but the detailed mechanisms remain unresolved.
34896162	4	10	theme	forced	880:885	arg1	test					897:900	a forced swim (FS) test	878:900	a forced swim (FS) test	878:900	KEY FINDINGS In naïve mice exposed to CUMS, their immobility times in a forced swim (FS) test were almost equal to those in control mice.
34896162	8	11	theme	microbiota	1795:1804	arg1	characteristics					1768:1782	altered characteristics	1760:1782	altered characteristics of the gut microbiota	1760:1804	SIGNIFICANCE These findings indicate that mice with colitis exhibit increased susceptibility to psychological stress, resulting in induction of depressive-like behavior, and this might be due, at least in part, to altered characteristics of the gut microbiota.
34896162	3	12	theme	MAIN	499:502	arg1	METHODS					504:510	MAIN METHODS	499:510	MAIN METHODS	499:510	MAIN METHODS To induce experimental colitis and depressive-like behaviors, male 7-weeks old C57BL/6J mice were administered ad libitum 1% DSS solution for 11 days, and subjected to various mild stressors in a chronic, inevitable and unpredictable way according to a random schedule for 21 days, respectively.
34896162	6	13	with	mice	1292:1295	arg1	colitis					1314:1320	DSS-induced colitis	1302:1320	DSS-induced colitis	1302:1320	Exposure of mice with DSS-induced colitis to CUMS (DSS + CUMS) significantly increased the immobility times in the FS test.
34896162	1	14	theme	disorders	229:237	arg1	colitis					185:191	colitis	185:191	colitis	185:191	AIMS In patients with colitis, the high comorbidity of depressive disorders is well-known, but the detailed mechanisms remain unresolved.
34896162	1	14	theme	disorders	229:237	arg1	comorbidity					203:213	the high comorbidity	194:213	the high comorbidity of depressive disorders	194:237	AIMS In patients with colitis, the high comorbidity of depressive disorders is well-known, but the detailed mechanisms remain unresolved.
34896162	4	15	theme	immobility	858:867	arg1	times					869:873	their immobility times	852:873	their immobility times in a forced swim (FS) test	852:900	KEY FINDINGS In naïve mice exposed to CUMS, their immobility times in a forced swim (FS) test were almost equal to those in control mice.
34896162	4	15	theme	immobility	858:867	arg1	CUMS					846:849	CUMS	846:849	CUMS	846:849	KEY FINDINGS In naïve mice exposed to CUMS, their immobility times in a forced swim (FS) test were almost equal to those in control mice.
34896162	8	16	from	due	1734:1736	arg1	part					1751:1754	part	1751:1754	part	1751:1754	SIGNIFICANCE These findings indicate that mice with colitis exhibit increased susceptibility to psychological stress, resulting in induction of depressive-like behavior, and this might be due, at least in part, to altered characteristics of the gut microbiota.
34896162	5	17	theme	naïve	972:976	arg1	mice					978:981	naïve mice	972:981	naïve mice	972:981	The DSS administration to naïve mice induced colitis without depressive-like behavior, and at 18 days after termination of the DSS administration, the colitis had recovered to control levels, while altered diversity and composition of bacterial genera such as Bacteroides spp., Alistipes spp., etc., were found in the gut microbiota.
34896162	3	18	theme	old	587:589	arg1	mice					600:603	male 7-weeks old C57BL/6J mice	574:603	male 7-weeks old C57BL/6J mice	574:603	MAIN METHODS To induce experimental colitis and depressive-like behaviors, male 7-weeks old C57BL/6J mice were administered ad libitum 1% DSS solution for 11 days, and subjected to various mild stressors in a chronic, inevitable and unpredictable way according to a random schedule for 21 days, respectively.
34896162	7	19	theme	genera	1508:1513	arg1	abundance					1485:1493	the relative abundance	1472:1493	the relative abundance of bacterial genera	1472:1513	In the gut microbiota of DSS + CUMS mice, the alteration profile of the relative abundance of bacterial genera differed from in the DSS ones.
34896162	3	20	theme	libitum	626:632	arg1	solution					641:648	ad libitum 1% DSS solution	623:648	ad libitum 1% DSS solution for 11 days	623:660	MAIN METHODS To induce experimental colitis and depressive-like behaviors, male 7-weeks old C57BL/6J mice were administered ad libitum 1% DSS solution for 11 days, and subjected to various mild stressors in a chronic, inevitable and unpredictable way according to a random schedule for 21 days, respectively.
34896162	8	21	theme	behavior	1706:1713	arg1	induction					1677:1685	induction	1677:1685	induction of depressive-like behavior	1677:1713	SIGNIFICANCE These findings indicate that mice with colitis exhibit increased susceptibility to psychological stress, resulting in induction of depressive-like behavior, and this might be due, at least in part, to altered characteristics of the gut microbiota.
34896162	3	22	theme	random	765:770	arg1	schedule					772:779	a random schedule	763:779	a random schedule for 21 days	763:791	MAIN METHODS To induce experimental colitis and depressive-like behaviors, male 7-weeks old C57BL/6J mice were administered ad libitum 1% DSS solution for 11 days, and subjected to various mild stressors in a chronic, inevitable and unpredictable way according to a random schedule for 21 days, respectively.
34896162	7	23	theme	relative	1476:1483	arg1	abundance					1485:1493	the relative abundance	1472:1493	the relative abundance of bacterial genera	1472:1513	In the gut microbiota of DSS + CUMS mice, the alteration profile of the relative abundance of bacterial genera differed from in the DSS ones.
34896162	5	24	theme	genera	1191:1196	arg1	composition					1166:1176	composition	1166:1176	composition	1166:1176	The DSS administration to naïve mice induced colitis without depressive-like behavior, and at 18 days after termination of the DSS administration, the colitis had recovered to control levels, while altered diversity and composition of bacterial genera such as Bacteroides spp., Alistipes spp., etc., were found in the gut microbiota.
34896162	5	24	theme	genera	1191:1196	arg1	diversity					1152:1160	altered diversity	1144:1160	altered diversity	1144:1160	The DSS administration to naïve mice induced colitis without depressive-like behavior, and at 18 days after termination of the DSS administration, the colitis had recovered to control levels, while altered diversity and composition of bacterial genera such as Bacteroides spp., Alistipes spp., etc., were found in the gut microbiota.
34896162	0	25	theme	unpredictable	98:110	arg1	stress					117:122	chronic unpredictable mild stress	90:122	chronic unpredictable mild stress that induces depressive-like behavior	90:160	Dextran sulfate sodium-induced colitis in C57BL/6J mice increases their susceptibility to chronic unpredictable mild stress that induces depressive-like behavior.
34896162	1	26	from	AIMS	163:166	arg1	patients					171:178	patients	171:178	patients with colitis, the high comorbidity of depressive disorders	171:237	AIMS In patients with colitis, the high comorbidity of depressive disorders is well-known, but the detailed mechanisms remain unresolved.
34896162	7	27	theme	alteration	1450:1459	arg1	profile					1461:1467	the alteration profile	1446:1467	the alteration profile of the relative abundance of bacterial genera	1446:1513	In the gut microbiota of DSS + CUMS mice, the alteration profile of the relative abundance of bacterial genera differed from in the DSS ones.
34896162	0	28	theme	Dextran	0:6	arg1	sulfate					8:14	Dextran sulfate	0:14	Dextran sulfate sodium-induced colitis in C57BL/6J mice	0:54	Dextran sulfate sodium-induced colitis in C57BL/6J mice increases their susceptibility to chronic unpredictable mild stress that induces depressive-like behavior.
34896162	5	29	located	found	1251:1255	arg2	composition					1166:1176	composition	1166:1176	composition	1166:1176	The DSS administration to naïve mice induced colitis without depressive-like behavior, and at 18 days after termination of the DSS administration, the colitis had recovered to control levels, while altered diversity and composition of bacterial genera such as Bacteroides spp., Alistipes spp., etc., were found in the gut microbiota.
34896162	5	29	located	found	1251:1255	arg1	microbiota					1268:1277	the gut microbiota	1260:1277	the gut microbiota	1260:1277	The DSS administration to naïve mice induced colitis without depressive-like behavior, and at 18 days after termination of the DSS administration, the colitis had recovered to control levels, while altered diversity and composition of bacterial genera such as Bacteroides spp., Alistipes spp., etc., were found in the gut microbiota.
34896162	5	29	located	found	1251:1255	arg2	diversity					1152:1160	altered diversity	1144:1160	altered diversity	1144:1160	The DSS administration to naïve mice induced colitis without depressive-like behavior, and at 18 days after termination of the DSS administration, the colitis had recovered to control levels, while altered diversity and composition of bacterial genera such as Bacteroides spp., Alistipes spp., etc., were found in the gut microbiota.
34896162	2	30	theme	unpredictable	424:436	arg1	CUMS					451:454	CUMS	451:454	CUMS	451:454	In this study, we examined whether colitis induced by dextran sulfate sodium (DSS) increased the susceptibility to chronic unpredictable mild stress (CUMS) in C57BL/6J mice with resilience to CUMS.
34896162	2	30	theme	unpredictable	424:436	arg1	stress					443:448	chronic unpredictable mild stress	416:448	chronic unpredictable mild stress (CUMS) in C57BL/6J mice with resilience	416:488	In this study, we examined whether colitis induced by dextran sulfate sodium (DSS) increased the susceptibility to chronic unpredictable mild stress (CUMS) in C57BL/6J mice with resilience to CUMS.
34896162	0	31	theme	sodium-induced	16:29	arg1	colitis					31:37	Dextran sulfate sodium-induced colitis	0:37	Dextran sulfate sodium-induced colitis in C57BL/6J mice	0:54	Dextran sulfate sodium-induced colitis in C57BL/6J mice increases their susceptibility to chronic unpredictable mild stress that induces depressive-like behavior.
34896162	7	32	theme	mice	1440:1443	arg1	microbiota					1415:1424	the gut microbiota	1407:1424	the gut microbiota of DSS + CUMS mice	1407:1443	In the gut microbiota of DSS + CUMS mice, the alteration profile of the relative abundance of bacterial genera differed from in the DSS ones.
34896162	4	33	theme	control	932:938	arg1	mice					940:943	control mice	932:943	control mice	932:943	KEY FINDINGS In naïve mice exposed to CUMS, their immobility times in a forced swim (FS) test were almost equal to those in control mice.
34896162	8	34	from	part	1751:1754	arg1	due					1734:1736	due	1734:1736	due	1734:1736	SIGNIFICANCE These findings indicate that mice with colitis exhibit increased susceptibility to psychological stress, resulting in induction of depressive-like behavior, and this might be due, at least in part, to altered characteristics of the gut microbiota.
34896162	5	35	theme	administration	1077:1090	arg1	termination					1054:1064	termination	1054:1064	termination of the DSS administration	1054:1090	The DSS administration to naïve mice induced colitis without depressive-like behavior, and at 18 days after termination of the DSS administration, the colitis had recovered to control levels, while altered diversity and composition of bacterial genera such as Bacteroides spp., Alistipes spp., etc., were found in the gut microbiota.
34896162	5	36	theme	DSS	950:952	arg1	administration					954:967	The DSS administration	946:967	The DSS administration to naïve mice	946:981	The DSS administration to naïve mice induced colitis without depressive-like behavior, and at 18 days after termination of the DSS administration, the colitis had recovered to control levels, while altered diversity and composition of bacterial genera such as Bacteroides spp., Alistipes spp., etc., were found in the gut microbiota.
34896162	3	37	theme	%	635:635	arg1	solution					641:648	ad libitum 1% DSS solution	623:648	ad libitum 1% DSS solution for 11 days	623:660	MAIN METHODS To induce experimental colitis and depressive-like behaviors, male 7-weeks old C57BL/6J mice were administered ad libitum 1% DSS solution for 11 days, and subjected to various mild stressors in a chronic, inevitable and unpredictable way according to a random schedule for 21 days, respectively.
34896162	4	38	theme	KEY	808:810	arg1	FINDINGS					812:819	KEY FINDINGS	808:819	KEY FINDINGS In naïve mice exposed to CUMS, their immobility times in a forced swim (FS) test	808:900	KEY FINDINGS In naïve mice exposed to CUMS, their immobility times in a forced swim (FS) test were almost equal to those in control mice.
34896162	2	39	from	stress	443:448	arg1	mice					469:472	C57BL/6J mice	460:472	C57BL/6J mice	460:472	In this study, we examined whether colitis induced by dextran sulfate sodium (DSS) increased the susceptibility to chronic unpredictable mild stress (CUMS) in C57BL/6J mice with resilience to CUMS.
34896162	7	40	theme	gut	1411:1413	arg1	microbiota					1415:1424	the gut microbiota	1407:1424	the gut microbiota of DSS + CUMS mice	1407:1443	In the gut microbiota of DSS + CUMS mice, the alteration profile of the relative abundance of bacterial genera differed from in the DSS ones.
34896162	6	41	from	times	1382:1386	arg1	test					1398:1401	the FS test	1391:1401	the FS test	1391:1401	Exposure of mice with DSS-induced colitis to CUMS (DSS + CUMS) significantly increased the immobility times in the FS test.
34896162	3	42	theme	mild	688:691	arg1	stressors					693:701	various mild stressors	680:701	various mild stressors	680:701	MAIN METHODS To induce experimental colitis and depressive-like behaviors, male 7-weeks old C57BL/6J mice were administered ad libitum 1% DSS solution for 11 days, and subjected to various mild stressors in a chronic, inevitable and unpredictable way according to a random schedule for 21 days, respectively.
34896162	2	43	theme	sulfate	363:369	arg1	DSS					379:381	DSS	379:381	DSS	379:381	In this study, we examined whether colitis induced by dextran sulfate sodium (DSS) increased the susceptibility to chronic unpredictable mild stress (CUMS) in C57BL/6J mice with resilience to CUMS.
34896162	2	43	theme	sulfate	363:369	arg1	sodium					371:376	dextran sulfate sodium	355:376	dextran sulfate sodium (DSS)	355:382	In this study, we examined whether colitis induced by dextran sulfate sodium (DSS) increased the susceptibility to chronic unpredictable mild stress (CUMS) in C57BL/6J mice with resilience to CUMS.
34896162	8	44	theme	altered	1760:1766	arg1	characteristics					1768:1782	altered characteristics	1760:1782	altered characteristics of the gut microbiota	1760:1804	SIGNIFICANCE These findings indicate that mice with colitis exhibit increased susceptibility to psychological stress, resulting in induction of depressive-like behavior, and this might be due, at least in part, to altered characteristics of the gut microbiota.
34896162	3	45	theme	chronic	708:714	arg1	way					746:748	a chronic, inevitable and unpredictable way	706:748	a chronic, inevitable and unpredictable way according to a random schedule for 21 days, respectively	706:805	MAIN METHODS To induce experimental colitis and depressive-like behaviors, male 7-weeks old C57BL/6J mice were administered ad libitum 1% DSS solution for 11 days, and subjected to various mild stressors in a chronic, inevitable and unpredictable way according to a random schedule for 21 days, respectively.
34896162	8	46	theme	gut	1791:1793	arg1	microbiota					1795:1804	the gut microbiota	1787:1804	the gut microbiota	1787:1804	SIGNIFICANCE These findings indicate that mice with colitis exhibit increased susceptibility to psychological stress, resulting in induction of depressive-like behavior, and this might be due, at least in part, to altered characteristics of the gut microbiota.
34896162	8	47	theme	increased	1614:1622	arg1	susceptibility					1624:1637	increased susceptibility	1614:1637	increased susceptibility to psychological stress	1614:1661	SIGNIFICANCE These findings indicate that mice with colitis exhibit increased susceptibility to psychological stress, resulting in induction of depressive-like behavior, and this might be due, at least in part, to altered characteristics of the gut microbiota.
34896162	7	48	theme	DSS	1536:1538	arg1	ones					1540:1543	the DSS ones	1532:1543	the DSS ones	1532:1543	In the gut microbiota of DSS + CUMS mice, the alteration profile of the relative abundance of bacterial genera differed from in the DSS ones.
34896162	4	49	theme	naïve	824:828	arg1	mice					830:833	naïve mice	824:833	naïve mice exposed to CUMS, their immobility times in a forced swim (FS) test	824:900	KEY FINDINGS In naïve mice exposed to CUMS, their immobility times in a forced swim (FS) test were almost equal to those in control mice.
34896162	3	50	theme	unpredictable	732:744	arg1	way					746:748	a chronic, inevitable and unpredictable way	706:748	a chronic, inevitable and unpredictable way according to a random schedule for 21 days, respectively	706:805	MAIN METHODS To induce experimental colitis and depressive-like behaviors, male 7-weeks old C57BL/6J mice were administered ad libitum 1% DSS solution for 11 days, and subjected to various mild stressors in a chronic, inevitable and unpredictable way according to a random schedule for 21 days, respectively.
34896162	0	51	theme	depressive-like	137:151	arg1	behavior					153:160	depressive-like behavior	137:160	depressive-like behavior	137:160	Dextran sulfate sodium-induced colitis in C57BL/6J mice increases their susceptibility to chronic unpredictable mild stress that induces depressive-like behavior.
34896162	3	52	theme	depressive-like	547:561	arg1	behaviors					563:571	depressive-like behaviors	547:571	depressive-like behaviors	547:571	MAIN METHODS To induce experimental colitis and depressive-like behaviors, male 7-weeks old C57BL/6J mice were administered ad libitum 1% DSS solution for 11 days, and subjected to various mild stressors in a chronic, inevitable and unpredictable way according to a random schedule for 21 days, respectively.
34896162	3	53	theme	inevitable	717:726	arg1	way					746:748	a chronic, inevitable and unpredictable way	706:748	a chronic, inevitable and unpredictable way according to a random schedule for 21 days, respectively	706:805	MAIN METHODS To induce experimental colitis and depressive-like behaviors, male 7-weeks old C57BL/6J mice were administered ad libitum 1% DSS solution for 11 days, and subjected to various mild stressors in a chronic, inevitable and unpredictable way according to a random schedule for 21 days, respectively.
34896162	5	54	theme	depressive-like	1007:1021	arg1	behavior					1023:1030	depressive-like behavior	1007:1030	depressive-like behavior	1007:1030	The DSS administration to naïve mice induced colitis without depressive-like behavior, and at 18 days after termination of the DSS administration, the colitis had recovered to control levels, while altered diversity and composition of bacterial genera such as Bacteroides spp., Alistipes spp., etc., were found in the gut microbiota.
34896162	3	55	theme	C57BL/6J	591:598	arg1	mice					600:603	male 7-weeks old C57BL/6J mice	574:603	male 7-weeks old C57BL/6J mice	574:603	MAIN METHODS To induce experimental colitis and depressive-like behaviors, male 7-weeks old C57BL/6J mice were administered ad libitum 1% DSS solution for 11 days, and subjected to various mild stressors in a chronic, inevitable and unpredictable way according to a random schedule for 21 days, respectively.
34896162	1	56	theme	depressive	218:227	arg1	disorders					229:237	depressive disorders	218:237	depressive disorders	218:237	AIMS In patients with colitis, the high comorbidity of depressive disorders is well-known, but the detailed mechanisms remain unresolved.
34896162	4	57	theme	swim	887:890	arg1	test					897:900	a forced swim (FS) test	878:900	a forced swim (FS) test	878:900	KEY FINDINGS In naïve mice exposed to CUMS, their immobility times in a forced swim (FS) test were almost equal to those in control mice.
34896162	3	58	theme	7-weeks	579:585	arg1	mice					600:603	male 7-weeks old C57BL/6J mice	574:603	male 7-weeks old C57BL/6J mice	574:603	MAIN METHODS To induce experimental colitis and depressive-like behaviors, male 7-weeks old C57BL/6J mice were administered ad libitum 1% DSS solution for 11 days, and subjected to various mild stressors in a chronic, inevitable and unpredictable way according to a random schedule for 21 days, respectively.
34896162	7	59	theme	bacterial	1498:1506	arg1	genera					1508:1513	bacterial genera	1498:1513	bacterial genera	1498:1513	In the gut microbiota of DSS + CUMS mice, the alteration profile of the relative abundance of bacterial genera differed from in the DSS ones.
34896162	4	60	from	times	869:873	arg1	test					897:900	a forced swim (FS) test	878:900	a forced swim (FS) test	878:900	KEY FINDINGS In naïve mice exposed to CUMS, their immobility times in a forced swim (FS) test were almost equal to those in control mice.
34896162	3	61	theme	ad	623:624	arg1	libitum					626:632	ad libitum 1	623:634	ad libitum 1% DSS solution for 11 days	623:660	MAIN METHODS To induce experimental colitis and depressive-like behaviors, male 7-weeks old C57BL/6J mice were administered ad libitum 1% DSS solution for 11 days, and subjected to various mild stressors in a chronic, inevitable and unpredictable way according to a random schedule for 21 days, respectively.
34896162	7	62	theme	abundance	1485:1493	arg1	profile					1461:1467	the alteration profile	1446:1467	the alteration profile of the relative abundance of bacterial genera	1446:1513	In the gut microbiota of DSS + CUMS mice, the alteration profile of the relative abundance of bacterial genera differed from in the DSS ones.
34896162	6	63	theme	DSS-induced	1302:1312	arg1	colitis					1314:1320	DSS-induced colitis	1302:1320	DSS-induced colitis	1302:1320	Exposure of mice with DSS-induced colitis to CUMS (DSS + CUMS) significantly increased the immobility times in the FS test.
34896162	8	64	theme	depressive-like	1690:1704	arg1	behavior					1706:1713	depressive-like behavior	1690:1713	depressive-like behavior	1690:1713	SIGNIFICANCE These findings indicate that mice with colitis exhibit increased susceptibility to psychological stress, resulting in induction of depressive-like behavior, and this might be due, at least in part, to altered characteristics of the gut microbiota.
34896162	0	65	from	colitis	31:37	arg1	mice					51:54	C57BL/6J mice	42:54	C57BL/6J mice	42:54	Dextran sulfate sodium-induced colitis in C57BL/6J mice increases their susceptibility to chronic unpredictable mild stress that induces depressive-like behavior.
34896162	8	66	dep	SIGNIFICANCE	1546:1557	arg1	indicate					1574:1581	indicate	1574:1581	indicate that mice with colitis exhibit increased susceptibility to psychological stress, resulting in induction of depressive-like behavior, and this might be due, at least in part, to altered characteristics of the gut microbiota	1574:1804	SIGNIFICANCE These findings indicate that mice with colitis exhibit increased susceptibility to psychological stress, resulting in induction of depressive-like behavior, and this might be due, at least in part, to altered characteristics of the gut microbiota.
34896162	5	67	theme	bacterial	1181:1189	arg1	genera					1191:1196	bacterial genera	1181:1196	bacterial genera such as Bacteroides spp., Alistipes spp., etc.,	1181:1244	The DSS administration to naïve mice induced colitis without depressive-like behavior, and at 18 days after termination of the DSS administration, the colitis had recovered to control levels, while altered diversity and composition of bacterial genera such as Bacteroides spp., Alistipes spp., etc., were found in the gut microbiota.
34896162	0	68	theme	chronic	90:96	arg1	stress					117:122	chronic unpredictable mild stress	90:122	chronic unpredictable mild stress that induces depressive-like behavior	90:160	Dextran sulfate sodium-induced colitis in C57BL/6J mice increases their susceptibility to chronic unpredictable mild stress that induces depressive-like behavior.
34896162	1	69	with	patients	171:178	arg1	colitis					185:191	colitis	185:191	colitis	185:191	AIMS In patients with colitis, the high comorbidity of depressive disorders is well-known, but the detailed mechanisms remain unresolved.
34896162	1	69	with	patients	171:178	arg1	comorbidity					203:213	the high comorbidity	194:213	the high comorbidity of depressive disorders	194:237	AIMS In patients with colitis, the high comorbidity of depressive disorders is well-known, but the detailed mechanisms remain unresolved.
34896162	0	70	theme	mild	112:115	arg1	stress					117:122	chronic unpredictable mild stress	90:122	chronic unpredictable mild stress that induces depressive-like behavior	90:160	Dextran sulfate sodium-induced colitis in C57BL/6J mice increases their susceptibility to chronic unpredictable mild stress that induces depressive-like behavior.
34896162	5	71	theme	DSS	1073:1075	arg1	administration					1077:1090	the DSS administration	1069:1090	the DSS administration	1069:1090	The DSS administration to naïve mice induced colitis without depressive-like behavior, and at 18 days after termination of the DSS administration, the colitis had recovered to control levels, while altered diversity and composition of bacterial genera such as Bacteroides spp., Alistipes spp., etc., were found in the gut microbiota.
34896162	4	72	theme	FS	893:894	arg1	test					897:900	a forced swim (FS) test	878:900	a forced swim (FS) test	878:900	KEY FINDINGS In naïve mice exposed to CUMS, their immobility times in a forced swim (FS) test were almost equal to those in control mice.
34896162	0	73	theme	sulfate	8:14	arg1	colitis					31:37	Dextran sulfate sodium-induced colitis	0:37	Dextran sulfate sodium-induced colitis in C57BL/6J mice	0:54	Dextran sulfate sodium-induced colitis in C57BL/6J mice increases their susceptibility to chronic unpredictable mild stress that induces depressive-like behavior.
34896162	4	74	from	FINDINGS	812:819	arg1	mice					830:833	naïve mice	824:833	naïve mice exposed to CUMS, their immobility times in a forced swim (FS) test	824:900	KEY FINDINGS In naïve mice exposed to CUMS, their immobility times in a forced swim (FS) test were almost equal to those in control mice.
34896162	2	75	theme	mild	438:441	arg1	CUMS					451:454	CUMS	451:454	CUMS	451:454	In this study, we examined whether colitis induced by dextran sulfate sodium (DSS) increased the susceptibility to chronic unpredictable mild stress (CUMS) in C57BL/6J mice with resilience to CUMS.
34896162	2	75	theme	mild	438:441	arg1	stress					443:448	chronic unpredictable mild stress	416:448	chronic unpredictable mild stress (CUMS) in C57BL/6J mice with resilience	416:488	In this study, we examined whether colitis induced by dextran sulfate sodium (DSS) increased the susceptibility to chronic unpredictable mild stress (CUMS) in C57BL/6J mice with resilience to CUMS.
34896162	3	76	theme	DSS	637:639	arg1	solution					641:648	ad libitum 1% DSS solution	623:648	ad libitum 1% DSS solution for 11 days	623:660	MAIN METHODS To induce experimental colitis and depressive-like behaviors, male 7-weeks old C57BL/6J mice were administered ad libitum 1% DSS solution for 11 days, and subjected to various mild stressors in a chronic, inevitable and unpredictable way according to a random schedule for 21 days, respectively.
34896162	2	77	theme	chronic	416:422	arg1	CUMS					451:454	CUMS	451:454	CUMS	451:454	In this study, we examined whether colitis induced by dextran sulfate sodium (DSS) increased the susceptibility to chronic unpredictable mild stress (CUMS) in C57BL/6J mice with resilience to CUMS.
34896162	2	77	theme	chronic	416:422	arg1	stress					443:448	chronic unpredictable mild stress	416:448	chronic unpredictable mild stress (CUMS) in C57BL/6J mice with resilience	416:488	In this study, we examined whether colitis induced by dextran sulfate sodium (DSS) increased the susceptibility to chronic unpredictable mild stress (CUMS) in C57BL/6J mice with resilience to CUMS.
34896162	0	78	theme	C57BL/6J	42:49	arg1	mice					51:54	C57BL/6J mice	42:54	C57BL/6J mice	42:54	Dextran sulfate sodium-induced colitis in C57BL/6J mice increases their susceptibility to chronic unpredictable mild stress that induces depressive-like behavior.
34896162	5	79	theme	control	1122:1128	arg1	levels					1130:1135	control levels	1122:1135	control levels	1122:1135	The DSS administration to naïve mice induced colitis without depressive-like behavior, and at 18 days after termination of the DSS administration, the colitis had recovered to control levels, while altered diversity and composition of bacterial genera such as Bacteroides spp., Alistipes spp., etc., were found in the gut microbiota.
34896162	2	80	theme	C57BL/6J	460:467	arg1	mice					469:472	C57BL/6J mice	460:472	C57BL/6J mice	460:472	In this study, we examined whether colitis induced by dextran sulfate sodium (DSS) increased the susceptibility to chronic unpredictable mild stress (CUMS) in C57BL/6J mice with resilience to CUMS.
34896162	7	81	theme	DSS + CUMS	1429:1438	arg1	mice					1440:1443	DSS + CUMS mice	1429:1443	DSS + CUMS mice	1429:1443	In the gut microbiota of DSS + CUMS mice, the alteration profile of the relative abundance of bacterial genera differed from in the DSS ones.
34896162	8	82	with	mice	1588:1591	arg1	colitis					1598:1604	colitis	1598:1604	colitis	1598:1604	SIGNIFICANCE These findings indicate that mice with colitis exhibit increased susceptibility to psychological stress, resulting in induction of depressive-like behavior, and this might be due, at least in part, to altered characteristics of the gut microbiota.
34896162	2	83	with	stress	443:448	arg1	resilience					479:488	resilience	479:488	resilience	479:488	In this study, we examined whether colitis induced by dextran sulfate sodium (DSS) increased the susceptibility to chronic unpredictable mild stress (CUMS) in C57BL/6J mice with resilience to CUMS.
34896162	5	84	theme	gut	1264:1266	arg1	microbiota					1268:1277	the gut microbiota	1260:1277	the gut microbiota	1260:1277	The DSS administration to naïve mice induced colitis without depressive-like behavior, and at 18 days after termination of the DSS administration, the colitis had recovered to control levels, while altered diversity and composition of bacterial genera such as Bacteroides spp., Alistipes spp., etc., were found in the gut microbiota.
34896162	6	85	theme	FS	1395:1396	arg1	test					1398:1401	the FS test	1391:1401	the FS test	1391:1401	Exposure of mice with DSS-induced colitis to CUMS (DSS + CUMS) significantly increased the immobility times in the FS test.
34896162	3	86	theme	various	680:686	arg1	stressors					693:701	various mild stressors	680:701	various mild stressors	680:701	MAIN METHODS To induce experimental colitis and depressive-like behaviors, male 7-weeks old C57BL/6J mice were administered ad libitum 1% DSS solution for 11 days, and subjected to various mild stressors in a chronic, inevitable and unpredictable way according to a random schedule for 21 days, respectively.
33838005	10	0	theme	sciatic	1984:1990	arg1	defects					1998:2004	10-mm sciatic nerve defects	1978:2004	10-mm sciatic nerve defects	1978:2004	CONCLUSIONS Nerve grafts comprising chitin biological conduits combined with SANs and PRP significantly improved the repair of 10-mm sciatic nerve defects in rats and may have therapeutic potential for repairing peripheral nerve defects in future applications.
33838005	2	1	theme	nerve	320:324	arg1	defects					326:332	repairing peripheral nerve defects	299:332	repairing peripheral nerve defects	299:332	Therefore, it is important to explore new methods of repairing peripheral nerve defects.
33838005	1	2	theme	repair	231:236	arg1	method					238:243	no optimal repair method	220:243	no optimal repair method	220:243	AIMS Peripheral nerve defects are often difficult to recover from, and there is no optimal repair method.
33838005	6	3	theme	efficiency	845:854	arg1	MEPs					1095:1098	MEPs	1095:1098	MEPs	1095:1098	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	3	theme	efficiency	845:854	arg1	evaluation					993:1002	histological evaluation	980:1002	histological evaluation of the gastrocnemius muscle	980:1030	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	3	theme	efficiency	845:854	arg1	counts					902:907	counts	902:907	counts of regenerated myelinated fibers and observations of their morphology	902:977	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	3	theme	efficiency	845:854	arg1	measurements					817:828	measurements	817:828	measurements of axon growth efficiency	817:854	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	3	theme	efficiency	845:854	arg1	analysis					864:871	a gait analysis	857:871	a gait analysis	857:871	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	3	theme	efficiency	845:854	arg1	endplates					1084:1092	motor endplates	1078:1092	motor endplates (MEPs)	1078:1099	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	3	theme	efficiency	845:854	arg1	tests					895:899	electrophysiological tests	874:899	electrophysiological tests	874:899	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	3	theme	efficiency	845:854	arg1	tracing					1044:1050	retrograde tracing	1033:1050	retrograde tracing with Fluor-Gold (FG)	1033:1071	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	4	with	counts	902:907	arg1	Fluor-Gold					1057:1066	Fluor-Gold	1057:1066	Fluor-Gold (FG)	1057:1071	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	4	with	counts	902:907	arg1	FG					1069:1070	FG	1069:1070	FG	1069:1070	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	8	5	theme	tissue	1547:1552	arg1	terms					1391:1395	terms	1391:1395	terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation	1391:1586	The PRP-SAN group exhibited better recovery in terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation, compared with the PRP and SAN groups.
33838005	8	6	theme	thickness	1520:1528	arg1	terms					1391:1395	terms	1391:1395	terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation	1391:1586	The PRP-SAN group exhibited better recovery in terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation, compared with the PRP and SAN groups.
33838005	10	7	theme	peripheral	2063:2072	arg1	defects					2080:2086	peripheral nerve defects	2063:2086	peripheral nerve defects in future applications	2063:2109	CONCLUSIONS Nerve grafts comprising chitin biological conduits combined with SANs and PRP significantly improved the repair of 10-mm sciatic nerve defects in rats and may have therapeutic potential for repairing peripheral nerve defects in future applications.
33838005	6	8	theme	axon	833:836	arg1	efficiency					845:854	axon growth efficiency	833:854	axon growth efficiency	833:854	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	9	theme	distribution	1101:1112	arg1	analysis					1114:1121	distribution analysis	1101:1121	distribution analysis	1101:1121	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	10	theme	muscle	1025:1030	arg1	MEPs					1095:1098	MEPs	1095:1098	MEPs	1095:1098	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	10	theme	muscle	1025:1030	arg1	evaluation					993:1002	histological evaluation	980:1002	histological evaluation of the gastrocnemius muscle	980:1030	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	10	theme	muscle	1025:1030	arg1	counts					902:907	counts	902:907	counts of regenerated myelinated fibers and observations of their morphology	902:977	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	10	theme	muscle	1025:1030	arg1	measurements					817:828	measurements	817:828	measurements of axon growth efficiency	817:854	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	10	theme	muscle	1025:1030	arg1	analysis					864:871	a gait analysis	857:871	a gait analysis	857:871	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	10	theme	muscle	1025:1030	arg1	endplates					1084:1092	motor endplates	1078:1092	motor endplates (MEPs)	1078:1099	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	10	theme	muscle	1025:1030	arg1	tests					895:899	electrophysiological tests	874:899	electrophysiological tests	874:899	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	10	theme	muscle	1025:1030	arg1	tracing					1044:1050	retrograde tracing	1033:1050	retrograde tracing with Fluor-Gold (FG)	1033:1071	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	10	11	theme	defects	1998:2004	arg1	repair					1968:1973	the repair	1964:1973	the repair of 10-mm sciatic nerve defects in rats	1964:2012	CONCLUSIONS Nerve grafts comprising chitin biological conduits combined with SANs and PRP significantly improved the repair of 10-mm sciatic nerve defects in rats and may have therapeutic potential for repairing peripheral nerve defects in future applications.
33838005	6	12	with	tests	895:899	arg1	Fluor-Gold					1057:1066	Fluor-Gold	1057:1066	Fluor-Gold (FG)	1057:1071	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	12	with	tests	895:899	arg1	FG					1069:1070	FG	1069:1070	FG	1069:1070	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	10	13	theme	therapeutic	2027:2037	arg1	potential					2039:2047	therapeutic potential	2027:2047	therapeutic potential	2027:2047	CONCLUSIONS Nerve grafts comprising chitin biological conduits combined with SANs and PRP significantly improved the repair of 10-mm sciatic nerve defects in rats and may have therapeutic potential for repairing peripheral nerve defects in future applications.
33838005	5	14	theme	nerve	656:660	arg1	grafts					662:667	The nerve grafts	652:667	The nerve grafts	652:667	The nerve grafts consisted of chitin biological conduits combined with SAN and PRP, and were used to repair rat sciatic nerve defects.
33838005	3	15	theme	sciatic	524:530	arg1	defects					538:544	10-mm sciatic nerve defects	518:544	10-mm sciatic nerve defects in rats	518:552	This study explored the efficacy of nerve grafts constructed from chitin biological conduits combined with small autogenous nerves (SANs) and platelet-rich plasma (PRP) for repairing 10-mm sciatic nerve defects in rats.
33838005	5	16	theme	nerve	772:776	arg1	defects					778:784	repair rat sciatic nerve defects	753:784	repair rat sciatic nerve defects	753:784	The nerve grafts consisted of chitin biological conduits combined with SAN and PRP, and were used to repair rat sciatic nerve defects.
33838005	6	17	theme	gastrocnemius	1011:1023	arg1	muscle					1025:1030	the gastrocnemius muscle	1007:1030	the gastrocnemius muscle	1007:1030	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	18	theme	electrophysiological	874:893	arg1	tests					895:899	electrophysiological tests	874:899	electrophysiological tests	874:899	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	19	theme	repair	1155:1160	arg1	status					1162:1167	the repair status	1151:1167	the repair status	1151:1167	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	9	20	theme	SAN	1840:1842	arg1	group					1844:1848	the PRP or SAN group	1829:1848	group	1844:1848	The results of FG retrograde tracing and MEPs analyses showed that numbers of FG-positive sensory neurons and motor neurons as well as MEPs distribution density were higher in the PRP-SAN group than in the PRP or SAN group.
33838005	10	21	theme	Nerve	1863:1867	arg1	grafts					1869:1874	CONCLUSIONS Nerve grafts	1851:1874	CONCLUSIONS Nerve grafts comprising chitin biological conduits combined with SANs and PRP	1851:1939	CONCLUSIONS Nerve grafts comprising chitin biological conduits combined with SANs and PRP significantly improved the repair of 10-mm sciatic nerve defects in rats and may have therapeutic potential for repairing peripheral nerve defects in future applications.
33838005	8	22	theme	better	1372:1377	arg1	recovery					1379:1386	better recovery	1372:1386	better recovery	1372:1386	The PRP-SAN group exhibited better recovery in terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation, compared with the PRP and SAN groups.
33838005	6	23	theme	morphology	968:977	arg1	observations					946:957	observations	946:957	observations of their morphology	946:977	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	23	theme	morphology	968:977	arg1	fibers					935:940	regenerated myelinated fibers	912:940	regenerated myelinated fibers	912:940	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	9	24	theme	FG	1642:1643	arg1	tracing					1656:1662	FG retrograde tracing	1642:1662	FG retrograde tracing	1642:1662	The results of FG retrograde tracing and MEPs analyses showed that numbers of FG-positive sensory neurons and motor neurons as well as MEPs distribution density were higher in the PRP-SAN group than in the PRP or SAN group.
33838005	7	25	theme	PRP-SAN	1265:1271	arg1	group					1273:1277	the PRP-SAN group	1261:1277	the PRP-SAN group	1261:1277	RESULTS Two weeks after nerve transplantation, the rate and number of regenerated axons in the PRP-SAN group improved compared with those in the PRP, SAN, and Hollow groups.
33838005	10	26	theme	future	2091:2096	arg1	applications					2098:2109	future applications	2091:2109	future applications	2091:2109	CONCLUSIONS Nerve grafts comprising chitin biological conduits combined with SANs and PRP significantly improved the repair of 10-mm sciatic nerve defects in rats and may have therapeutic potential for repairing peripheral nerve defects in future applications.
33838005	3	27	from	defects	538:544	arg1	rats					549:552	rats	549:552	rats	549:552	This study explored the efficacy of nerve grafts constructed from chitin biological conduits combined with small autogenous nerves (SANs) and platelet-rich plasma (PRP) for repairing 10-mm sciatic nerve defects in rats.
33838005	10	28	theme	biological	1894:1903	arg1	conduits					1905:1912	chitin biological conduits	1887:1912	chitin biological conduits combined with SANs and PRP	1887:1939	CONCLUSIONS Nerve grafts comprising chitin biological conduits combined with SANs and PRP significantly improved the repair of 10-mm sciatic nerve defects in rats and may have therapeutic potential for repairing peripheral nerve defects in future applications.
33838005	5	29	theme	rat	760:762	arg1	nerve					772:776	repair rat sciatic nerve	753:776	repair rat sciatic nerve defects	753:784	The nerve grafts consisted of chitin biological conduits combined with SAN and PRP, and were used to repair rat sciatic nerve defects.
33838005	1	30	theme	AIMS	140:143	arg1	defects					162:168	AIMS Peripheral nerve defects	140:168	AIMS Peripheral nerve defects	140:168	AIMS Peripheral nerve defects are often difficult to recover from, and there is no optimal repair method.
33838005	9	31	theme	tracing	1656:1662	arg1	results					1631:1637	The results	1627:1637	The results of FG retrograde tracing and MEPs analyses	1627:1680	The results of FG retrograde tracing and MEPs analyses showed that numbers of FG-positive sensory neurons and motor neurons as well as MEPs distribution density were higher in the PRP-SAN group than in the PRP or SAN group.
33838005	1	32	theme	nerve	156:160	arg1	defects					162:168	AIMS Peripheral nerve defects	140:168	AIMS Peripheral nerve defects	140:168	AIMS Peripheral nerve defects are often difficult to recover from, and there is no optimal repair method.
33838005	9	33	theme	MEPs	1668:1671	arg1	results					1631:1637	The results	1627:1637	The results of FG retrograde tracing and MEPs analyses	1627:1680	The results of FG retrograde tracing and MEPs analyses showed that numbers of FG-positive sensory neurons and motor neurons as well as MEPs distribution density were higher in the PRP-SAN group than in the PRP or SAN group.
33838005	8	34	theme	sciatic	1404:1410	arg1	index					1423:1427	the sciatic functional index	1400:1427	the sciatic functional index value	1400:1433	The PRP-SAN group exhibited better recovery in terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation, compared with the PRP and SAN groups.
33838005	5	35	theme	chitin	682:687	arg1	conduits					700:707	chitin biological conduits	682:707	chitin biological conduits combined with SAN and PRP	682:733	The nerve grafts consisted of chitin biological conduits combined with SAN and PRP, and were used to repair rat sciatic nerve defects.
33838005	6	36	theme	myelinated	924:933	arg1	fibers					935:940	regenerated myelinated fibers	912:940	regenerated myelinated fibers	912:940	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	8	37	theme	index	1423:1427	arg1	value					1429:1433	the sciatic functional index value	1400:1433	the sciatic functional index value	1400:1433	The PRP-SAN group exhibited better recovery in terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation, compared with the PRP and SAN groups.
33838005	8	38	from	12 weeks	1557:1564	arg1	value					1429:1433	the sciatic functional index value	1400:1433	the sciatic functional index value	1400:1433	The PRP-SAN group exhibited better recovery in terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation, compared with the PRP and SAN groups.
33838005	8	38	from	12 weeks	1557:1564	arg1	thickness					1520:1528	myelin sheath thickness	1506:1528	myelin sheath thickness	1506:1528	The PRP-SAN group exhibited better recovery in terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation, compared with the PRP and SAN groups.
33838005	8	38	from	12 weeks	1557:1564	arg1	density					1497:1503	myelinated nerve fiber density	1474:1503	myelinated nerve fiber density	1474:1503	The PRP-SAN group exhibited better recovery in terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation, compared with the PRP and SAN groups.
33838005	8	38	from	12 weeks	1557:1564	arg1	tissue					1547:1552	gastrectomy tissue	1535:1552	gastrectomy tissue	1535:1552	The PRP-SAN group exhibited better recovery in terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation, compared with the PRP and SAN groups.
33838005	8	38	from	12 weeks	1557:1564	arg1	intensity					1463:1471	composite action potential intensity	1436:1471	composite action potential intensity	1436:1471	The PRP-SAN group exhibited better recovery in terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation, compared with the PRP and SAN groups.
33838005	8	38	from	12 weeks	1557:1564	arg1	terms					1391:1395	terms	1391:1395	terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation	1391:1586	The PRP-SAN group exhibited better recovery in terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation, compared with the PRP and SAN groups.
33838005	3	39	theme	biological	408:417	arg1	conduits					419:426	chitin biological conduits	401:426	chitin biological conduits combined with small autogenous nerves (SANs) and platelet-rich plasma (PRP)	401:502	This study explored the efficacy of nerve grafts constructed from chitin biological conduits combined with small autogenous nerves (SANs) and platelet-rich plasma (PRP) for repairing 10-mm sciatic nerve defects in rats.
33838005	9	40	theme	neurons	1725:1731	arg1	density					1780:1786	MEPs distribution density	1762:1786	numbers of FG-positive sensory neurons and motor neurons as well as MEPs distribution density	1694:1786	The results of FG retrograde tracing and MEPs analyses showed that numbers of FG-positive sensory neurons and motor neurons as well as MEPs distribution density were higher in the PRP-SAN group than in the PRP or SAN group.
33838005	9	40	theme	neurons	1725:1731	arg1	numbers					1694:1700	numbers	1694:1700	numbers of FG-positive sensory neurons and motor neurons as well as MEPs distribution density	1694:1786	The results of FG retrograde tracing and MEPs analyses showed that numbers of FG-positive sensory neurons and motor neurons as well as MEPs distribution density were higher in the PRP-SAN group than in the PRP or SAN group.
33838005	6	41	with	tracing	1044:1050	arg1	Fluor-Gold					1057:1066	Fluor-Gold	1057:1066	Fluor-Gold (FG)	1057:1071	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	41	with	tracing	1044:1050	arg1	FG					1069:1070	FG	1069:1070	FG	1069:1070	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	7	42	theme	axons	1252:1256	arg1	number					1230:1235	number	1230:1235	number	1230:1235	RESULTS Two weeks after nerve transplantation, the rate and number of regenerated axons in the PRP-SAN group improved compared with those in the PRP, SAN, and Hollow groups.
33838005	7	42	theme	axons	1252:1256	arg1	rate					1221:1224	rate	1221:1224	rate	1221:1224	RESULTS Two weeks after nerve transplantation, the rate and number of regenerated axons in the PRP-SAN group improved compared with those in the PRP, SAN, and Hollow groups.
33838005	9	43	theme	FG-positive	1705:1715	arg1	neurons					1725:1731	FG-positive sensory neurons	1705:1731	FG-positive sensory neurons	1705:1731	The results of FG retrograde tracing and MEPs analyses showed that numbers of FG-positive sensory neurons and motor neurons as well as MEPs distribution density were higher in the PRP-SAN group than in the PRP or SAN group.
33838005	8	44	theme	action	1446:1451	arg1	intensity					1463:1471	composite action potential intensity	1436:1471	composite action potential intensity	1436:1471	The PRP-SAN group exhibited better recovery in terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation, compared with the PRP and SAN groups.
33838005	3	45	theme	small	442:446	arg1	SANs					467:470	SANs	467:470	SANs	467:470	This study explored the efficacy of nerve grafts constructed from chitin biological conduits combined with small autogenous nerves (SANs) and platelet-rich plasma (PRP) for repairing 10-mm sciatic nerve defects in rats.
33838005	3	45	theme	small	442:446	arg1	nerves					459:464	small autogenous nerves	442:464	small autogenous nerves (SANs)	442:471	This study explored the efficacy of nerve grafts constructed from chitin biological conduits combined with small autogenous nerves (SANs) and platelet-rich plasma (PRP) for repairing 10-mm sciatic nerve defects in rats.
33838005	0	46	theme	biological	17:26	arg1	conduits					28:35	chitin biological conduits	10:35	chitin biological conduits	10:35	Combining chitin biological conduits with small autogenous nerves and platelet-rich plasma for the repair of sciatic nerve defects in rats.
33838005	3	47	theme	grafts	377:382	arg1	efficacy					359:366	the efficacy	355:366	the efficacy of nerve grafts constructed from chitin biological conduits combined with small autogenous nerves (SANs) and platelet-rich plasma (PRP) for repairing 10-mm sciatic nerve defects in rats	355:552	This study explored the efficacy of nerve grafts constructed from chitin biological conduits combined with small autogenous nerves (SANs) and platelet-rich plasma (PRP) for repairing 10-mm sciatic nerve defects in rats.
33838005	8	48	theme	intensity	1463:1471	arg1	terms					1391:1395	terms	1391:1395	terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation	1391:1586	The PRP-SAN group exhibited better recovery in terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation, compared with the PRP and SAN groups.
33838005	7	49	from	rate	1221:1224	arg1	group					1273:1277	the PRP-SAN group	1261:1277	the PRP-SAN group	1261:1277	RESULTS Two weeks after nerve transplantation, the rate and number of regenerated axons in the PRP-SAN group improved compared with those in the PRP, SAN, and Hollow groups.
33838005	6	50	theme	histological	980:991	arg1	evaluation					993:1002	histological evaluation	980:1002	histological evaluation of the gastrocnemius muscle	980:1030	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	9	51	theme	motor	1737:1741	arg1	neurons					1743:1749	motor neurons	1737:1749	motor neurons	1737:1749	The results of FG retrograde tracing and MEPs analyses showed that numbers of FG-positive sensory neurons and motor neurons as well as MEPs distribution density were higher in the PRP-SAN group than in the PRP or SAN group.
33838005	8	52	theme	myelinated	1474:1483	arg1	density					1497:1503	myelinated nerve fiber density	1474:1503	myelinated nerve fiber density	1474:1503	The PRP-SAN group exhibited better recovery in terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation, compared with the PRP and SAN groups.
33838005	2	53	theme	peripheral	309:318	arg1	defects					326:332	repairing peripheral nerve defects	299:332	repairing peripheral nerve defects	299:332	Therefore, it is important to explore new methods of repairing peripheral nerve defects.
33838005	0	54	theme	autogenous	48:57	arg1	nerves					59:64	small autogenous nerves	42:64	small autogenous nerves	42:64	Combining chitin biological conduits with small autogenous nerves and platelet-rich plasma for the repair of sciatic nerve defects in rats.
33838005	6	55	with	evaluation	993:1002	arg1	Fluor-Gold					1057:1066	Fluor-Gold	1057:1066	Fluor-Gold (FG)	1057:1071	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	55	with	evaluation	993:1002	arg1	FG					1069:1070	FG	1069:1070	FG	1069:1070	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	7	56	from	number	1230:1235	arg1	group					1273:1277	the PRP-SAN group	1261:1277	the PRP-SAN group	1261:1277	RESULTS Two weeks after nerve transplantation, the rate and number of regenerated axons in the PRP-SAN group improved compared with those in the PRP, SAN, and Hollow groups.
33838005	8	57	theme	fiber	1491:1495	arg1	density					1497:1503	myelinated nerve fiber density	1474:1503	myelinated nerve fiber density	1474:1503	The PRP-SAN group exhibited better recovery in terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation, compared with the PRP and SAN groups.
33838005	5	58	used	used	745:748	arg2	grafts					662:667	The nerve grafts	652:667	The nerve grafts	652:667	The nerve grafts consisted of chitin biological conduits combined with SAN and PRP, and were used to repair rat sciatic nerve defects.
33838005	6	59	dep	measurements	817:828	arg1	analysis					1114:1121	distribution analysis	1101:1121	distribution analysis	1101:1121	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	60	theme	gait	859:862	arg1	analysis					864:871	a gait analysis	857:871	a gait analysis	857:871	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	61	with	measurements	817:828	arg1	Fluor-Gold					1057:1066	Fluor-Gold	1057:1066	Fluor-Gold (FG)	1057:1071	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	61	with	measurements	817:828	arg1	FG					1069:1070	FG	1069:1070	FG	1069:1070	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	9	62	theme	distribution	1767:1778	arg1	density					1780:1786	MEPs distribution density	1762:1786	numbers of FG-positive sensory neurons and motor neurons as well as MEPs distribution density	1694:1786	The results of FG retrograde tracing and MEPs analyses showed that numbers of FG-positive sensory neurons and motor neurons as well as MEPs distribution density were higher in the PRP-SAN group than in the PRP or SAN group.
33838005	8	63	theme	sheath	1513:1518	arg1	thickness					1520:1528	myelin sheath thickness	1506:1528	myelin sheath thickness	1506:1528	The PRP-SAN group exhibited better recovery in terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation, compared with the PRP and SAN groups.
33838005	2	64	theme	defects	326:332	arg1	methods					288:294	new methods	284:294	new methods of repairing peripheral nerve defects	284:332	Therefore, it is important to explore new methods of repairing peripheral nerve defects.
33838005	1	65	theme	optimal	223:229	arg1	method					238:243	no optimal repair method	220:243	no optimal repair method	220:243	AIMS Peripheral nerve defects are often difficult to recover from, and there is no optimal repair method.
33838005	2	66	theme	new	284:286	arg1	methods					288:294	new methods	284:294	new methods of repairing peripheral nerve defects	284:332	Therefore, it is important to explore new methods of repairing peripheral nerve defects.
33838005	6	67	theme	growth	838:843	arg1	efficiency					845:854	axon growth efficiency	833:854	axon growth efficiency	833:854	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	8	68	theme	gastrectomy	1535:1545	arg1	tissue					1547:1552	gastrectomy tissue	1535:1552	gastrectomy tissue	1535:1552	The PRP-SAN group exhibited better recovery in terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation, compared with the PRP and SAN groups.
33838005	10	69	contain	have	2022:2025	arg2	potential					2039:2047	therapeutic potential	2027:2047	therapeutic potential	2027:2047	CONCLUSIONS Nerve grafts comprising chitin biological conduits combined with SANs and PRP significantly improved the repair of 10-mm sciatic nerve defects in rats and may have therapeutic potential for repairing peripheral nerve defects in future applications.
33838005	10	69	contain	have	2022:2025	arg1	grafts					1869:1874	CONCLUSIONS Nerve grafts	1851:1874	CONCLUSIONS Nerve grafts comprising chitin biological conduits combined with SANs and PRP	1851:1939	CONCLUSIONS Nerve grafts comprising chitin biological conduits combined with SANs and PRP significantly improved the repair of 10-mm sciatic nerve defects in rats and may have therapeutic potential for repairing peripheral nerve defects in future applications.
33838005	8	70	theme	PRP	1607:1609	arg1	groups					1619:1624	the PRP and SAN groups	1603:1624	groups	1619:1624	The PRP-SAN group exhibited better recovery in terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation, compared with the PRP and SAN groups.
33838005	9	71	theme	neurons	1743:1749	arg1	density					1780:1786	MEPs distribution density	1762:1786	numbers of FG-positive sensory neurons and motor neurons as well as MEPs distribution density	1694:1786	The results of FG retrograde tracing and MEPs analyses showed that numbers of FG-positive sensory neurons and motor neurons as well as MEPs distribution density were higher in the PRP-SAN group than in the PRP or SAN group.
33838005	9	71	theme	neurons	1743:1749	arg1	numbers					1694:1700	numbers	1694:1700	numbers of FG-positive sensory neurons and motor neurons as well as MEPs distribution density	1694:1786	The results of FG retrograde tracing and MEPs analyses showed that numbers of FG-positive sensory neurons and motor neurons as well as MEPs distribution density were higher in the PRP-SAN group than in the PRP or SAN group.
33838005	7	72	theme	Hollow	1329:1334	arg1	groups					1336:1341	the PRP, SAN, and Hollow groups	1311:1341	groups	1336:1341	RESULTS Two weeks after nerve transplantation, the rate and number of regenerated axons in the PRP-SAN group improved compared with those in the PRP, SAN, and Hollow groups.
33838005	9	73	theme	PRP-SAN	1807:1813	arg1	group					1815:1819	the PRP-SAN group	1803:1819	the PRP-SAN group	1803:1819	The results of FG retrograde tracing and MEPs analyses showed that numbers of FG-positive sensory neurons and motor neurons as well as MEPs distribution density were higher in the PRP-SAN group than in the PRP or SAN group.
33838005	3	74	theme	nerve	532:536	arg1	defects					538:544	10-mm sciatic nerve defects	518:544	10-mm sciatic nerve defects in rats	518:552	This study explored the efficacy of nerve grafts constructed from chitin biological conduits combined with small autogenous nerves (SANs) and platelet-rich plasma (PRP) for repairing 10-mm sciatic nerve defects in rats.
33838005	8	75	theme	SAN	1615:1617	arg1	groups					1619:1624	the PRP and SAN groups	1603:1624	groups	1619:1624	The PRP-SAN group exhibited better recovery in terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation, compared with the PRP and SAN groups.
33838005	6	76	theme	regenerated	912:922	arg1	fibers					935:940	regenerated myelinated fibers	912:940	regenerated myelinated fibers	912:940	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	77	with	analysis	864:871	arg1	Fluor-Gold					1057:1066	Fluor-Gold	1057:1066	Fluor-Gold (FG)	1057:1071	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	77	with	analysis	864:871	arg1	FG					1069:1070	FG	1069:1070	FG	1069:1070	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	0	78	theme	defects	123:129	arg1	repair					99:104	the repair	95:104	the repair of sciatic nerve defects in rats	95:137	Combining chitin biological conduits with small autogenous nerves and platelet-rich plasma for the repair of sciatic nerve defects in rats.
33838005	5	79	theme	sciatic	764:770	arg1	nerve					772:776	repair rat sciatic nerve	753:776	repair rat sciatic nerve defects	753:784	The nerve grafts consisted of chitin biological conduits combined with SAN and PRP, and were used to repair rat sciatic nerve defects.
33838005	8	80	theme	PRP-SAN	1348:1354	arg1	group					1356:1360	The PRP-SAN group	1344:1360	The PRP-SAN group	1344:1360	The PRP-SAN group exhibited better recovery in terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation, compared with the PRP and SAN groups.
33838005	6	81	theme	motor	1078:1082	arg1	MEPs					1095:1098	MEPs	1095:1098	MEPs	1095:1098	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	81	theme	motor	1078:1082	arg1	endplates					1084:1092	motor endplates	1078:1092	motor endplates (MEPs)	1078:1099	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	4	82	theme	nerve	588:592	arg1	defects					594:600	10-mm sciatic nerve defects	574:600	10-mm sciatic nerve defects	574:600	METHODS To prepare 10-mm sciatic nerve defects, SANs were first harvested and PRP was extracted.
33838005	6	83	with	endplates	1084:1092	arg1	Fluor-Gold					1057:1066	Fluor-Gold	1057:1066	Fluor-Gold (FG)	1057:1071	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	83	with	endplates	1084:1092	arg1	FG					1069:1070	FG	1069:1070	FG	1069:1070	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	10	84	theme	nerve	2074:2078	arg1	defects					2080:2086	peripheral nerve defects	2063:2086	peripheral nerve defects in future applications	2063:2109	CONCLUSIONS Nerve grafts comprising chitin biological conduits combined with SANs and PRP significantly improved the repair of 10-mm sciatic nerve defects in rats and may have therapeutic potential for repairing peripheral nerve defects in future applications.
33838005	9	85	dep	tracing	1656:1662	arg1	analyses					1673:1680	analyses	1673:1680	analyses	1673:1680	The results of FG retrograde tracing and MEPs analyses showed that numbers of FG-positive sensory neurons and motor neurons as well as MEPs distribution density were higher in the PRP-SAN group than in the PRP or SAN group.
33838005	10	86	theme	CONCLUSIONS	1851:1861	arg1	grafts					1869:1874	CONCLUSIONS Nerve grafts	1851:1874	CONCLUSIONS Nerve grafts comprising chitin biological conduits combined with SANs and PRP	1851:1939	CONCLUSIONS Nerve grafts comprising chitin biological conduits combined with SANs and PRP significantly improved the repair of 10-mm sciatic nerve defects in rats and may have therapeutic potential for repairing peripheral nerve defects in future applications.
33838005	7	87	theme	PRP	1315:1317	arg1	SAN					1320:1322	the PRP, SAN, and Hollow groups	1311:1341	SAN	1320:1322	RESULTS Two weeks after nerve transplantation, the rate and number of regenerated axons in the PRP-SAN group improved compared with those in the PRP, SAN, and Hollow groups.
33838005	0	88	from	repair	99:104	arg1	rats					134:137	rats	134:137	rats	134:137	Combining chitin biological conduits with small autogenous nerves and platelet-rich plasma for the repair of sciatic nerve defects in rats.
33838005	0	89	theme	platelet-rich	70:82	arg1	plasma					84:89	platelet-rich plasma	70:89	platelet-rich plasma	70:89	Combining chitin biological conduits with small autogenous nerves and platelet-rich plasma for the repair of sciatic nerve defects in rats.
33838005	9	90	theme	retrograde	1645:1654	arg1	tracing					1656:1662	FG retrograde tracing	1642:1662	FG retrograde tracing	1642:1662	The results of FG retrograde tracing and MEPs analyses showed that numbers of FG-positive sensory neurons and motor neurons as well as MEPs distribution density were higher in the PRP-SAN group than in the PRP or SAN group.
33838005	8	91	theme	myelin	1506:1511	arg1	sheath					1513:1518	myelin sheath	1506:1518	myelin sheath thickness	1506:1528	The PRP-SAN group exhibited better recovery in terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation, compared with the PRP and SAN groups.
33838005	10	92	theme	chitin	1887:1892	arg1	conduits					1905:1912	chitin biological conduits	1887:1912	chitin biological conduits combined with SANs and PRP	1887:1939	CONCLUSIONS Nerve grafts comprising chitin biological conduits combined with SANs and PRP significantly improved the repair of 10-mm sciatic nerve defects in rats and may have therapeutic potential for repairing peripheral nerve defects in future applications.
33838005	1	93	theme	Peripheral	145:154	arg1	defects					162:168	AIMS Peripheral nerve defects	140:168	AIMS Peripheral nerve defects	140:168	AIMS Peripheral nerve defects are often difficult to recover from, and there is no optimal repair method.
33838005	6	94	theme	observations	946:957	arg1	MEPs					1095:1098	MEPs	1095:1098	MEPs	1095:1098	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	94	theme	observations	946:957	arg1	evaluation					993:1002	histological evaluation	980:1002	histological evaluation of the gastrocnemius muscle	980:1030	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	94	theme	observations	946:957	arg1	counts					902:907	counts	902:907	counts of regenerated myelinated fibers and observations of their morphology	902:977	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	94	theme	observations	946:957	arg1	measurements					817:828	measurements	817:828	measurements of axon growth efficiency	817:854	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	94	theme	observations	946:957	arg1	analysis					864:871	a gait analysis	857:871	a gait analysis	857:871	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	94	theme	observations	946:957	arg1	endplates					1084:1092	motor endplates	1078:1092	motor endplates (MEPs)	1078:1099	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	94	theme	observations	946:957	arg1	tests					895:899	electrophysiological tests	874:899	electrophysiological tests	874:899	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	94	theme	observations	946:957	arg1	tracing					1044:1050	retrograde tracing	1033:1050	retrograde tracing with Fluor-Gold (FG)	1033:1071	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	0	95	theme	nerve	117:121	arg1	defects					123:129	sciatic nerve defects	109:129	sciatic nerve defects in rats	109:137	Combining chitin biological conduits with small autogenous nerves and platelet-rich plasma for the repair of sciatic nerve defects in rats.
33838005	8	96	theme	functional	1412:1421	arg1	index					1423:1427	the sciatic functional index	1400:1427	the sciatic functional index value	1400:1433	The PRP-SAN group exhibited better recovery in terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation, compared with the PRP and SAN groups.
33838005	8	97	from	terms	1391:1395	arg1	12 weeks					1557:1564	12 weeks	1557:1564	12 weeks after transplantation	1557:1586	The PRP-SAN group exhibited better recovery in terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation, compared with the PRP and SAN groups.
33838005	6	98	theme	fibers	935:940	arg1	MEPs					1095:1098	MEPs	1095:1098	MEPs	1095:1098	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	98	theme	fibers	935:940	arg1	evaluation					993:1002	histological evaluation	980:1002	histological evaluation of the gastrocnemius muscle	980:1030	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	98	theme	fibers	935:940	arg1	counts					902:907	counts	902:907	counts of regenerated myelinated fibers and observations of their morphology	902:977	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	98	theme	fibers	935:940	arg1	measurements					817:828	measurements	817:828	measurements of axon growth efficiency	817:854	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	98	theme	fibers	935:940	arg1	analysis					864:871	a gait analysis	857:871	a gait analysis	857:871	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	98	theme	fibers	935:940	arg1	endplates					1084:1092	motor endplates	1078:1092	motor endplates (MEPs)	1078:1099	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	98	theme	fibers	935:940	arg1	tests					895:899	electrophysiological tests	874:899	electrophysiological tests	874:899	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	6	98	theme	fibers	935:940	arg1	tracing					1044:1050	retrograde tracing	1033:1050	retrograde tracing with Fluor-Gold (FG)	1033:1071	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	8	99	theme	value	1429:1433	arg1	terms					1391:1395	terms	1391:1395	terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation	1391:1586	The PRP-SAN group exhibited better recovery in terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation, compared with the PRP and SAN groups.
33838005	0	100	theme	sciatic	109:115	arg1	defects					123:129	sciatic nerve defects	109:129	sciatic nerve defects in rats	109:137	Combining chitin biological conduits with small autogenous nerves and platelet-rich plasma for the repair of sciatic nerve defects in rats.
33838005	10	101	from	defects	2080:2086	arg1	applications					2098:2109	future applications	2091:2109	future applications	2091:2109	CONCLUSIONS Nerve grafts comprising chitin biological conduits combined with SANs and PRP significantly improved the repair of 10-mm sciatic nerve defects in rats and may have therapeutic potential for repairing peripheral nerve defects in future applications.
33838005	6	102	theme	retrograde	1033:1042	arg1	tracing					1044:1050	retrograde tracing	1033:1050	retrograde tracing with Fluor-Gold (FG)	1033:1071	These examinations, including measurements of axon growth efficiency, a gait analysis, electrophysiological tests, counts of regenerated myelinated fibers and observations of their morphology, histological evaluation of the gastrocnemius muscle, retrograde tracing with Fluor-Gold (FG), and motor endplates (MEPs) distribution analysis, were conducted to evaluate the repair status.
33838005	8	103	theme	composite	1436:1444	arg1	intensity					1463:1471	composite action potential intensity	1436:1471	composite action potential intensity	1436:1471	The PRP-SAN group exhibited better recovery in terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation, compared with the PRP and SAN groups.
33838005	5	104	theme	biological	689:698	arg1	conduits					700:707	chitin biological conduits	682:707	chitin biological conduits combined with SAN and PRP	682:733	The nerve grafts consisted of chitin biological conduits combined with SAN and PRP, and were used to repair rat sciatic nerve defects.
33838005	0	105	theme	chitin	10:15	arg1	conduits					28:35	chitin biological conduits	10:35	chitin biological conduits	10:35	Combining chitin biological conduits with small autogenous nerves and platelet-rich plasma for the repair of sciatic nerve defects in rats.
33838005	7	106	theme	regenerated	1240:1250	arg1	axons					1252:1256	regenerated axons	1240:1256	regenerated axons	1240:1256	RESULTS Two weeks after nerve transplantation, the rate and number of regenerated axons in the PRP-SAN group improved compared with those in the PRP, SAN, and Hollow groups.
33838005	8	107	theme	potential	1453:1461	arg1	intensity					1463:1471	composite action potential intensity	1436:1471	composite action potential intensity	1436:1471	The PRP-SAN group exhibited better recovery in terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation, compared with the PRP and SAN groups.
33838005	3	108	theme	chitin	401:406	arg1	conduits					419:426	chitin biological conduits	401:426	chitin biological conduits combined with small autogenous nerves (SANs) and platelet-rich plasma (PRP)	401:502	This study explored the efficacy of nerve grafts constructed from chitin biological conduits combined with small autogenous nerves (SANs) and platelet-rich plasma (PRP) for repairing 10-mm sciatic nerve defects in rats.
33838005	5	109	theme	repair	753:758	arg1	nerve					772:776	repair rat sciatic nerve	753:776	repair rat sciatic nerve defects	753:784	The nerve grafts consisted of chitin biological conduits combined with SAN and PRP, and were used to repair rat sciatic nerve defects.
33838005	7	110	dep	weeks	1182:1186	arg1	transplantation					1200:1214	nerve transplantation	1194:1214	nerve transplantation	1194:1214	RESULTS Two weeks after nerve transplantation, the rate and number of regenerated axons in the PRP-SAN group improved compared with those in the PRP, SAN, and Hollow groups.
33838005	9	111	theme	sensory	1717:1723	arg1	neurons					1725:1731	FG-positive sensory neurons	1705:1731	FG-positive sensory neurons	1705:1731	The results of FG retrograde tracing and MEPs analyses showed that numbers of FG-positive sensory neurons and motor neurons as well as MEPs distribution density were higher in the PRP-SAN group than in the PRP or SAN group.
33838005	10	112	from	repair	1968:1973	arg1	rats					2009:2012	rats	2009:2012	rats	2009:2012	CONCLUSIONS Nerve grafts comprising chitin biological conduits combined with SANs and PRP significantly improved the repair of 10-mm sciatic nerve defects in rats and may have therapeutic potential for repairing peripheral nerve defects in future applications.
33838005	9	113	theme	PRP	1833:1835	arg1	group					1844:1848	the PRP or SAN group	1829:1848	group	1844:1848	The results of FG retrograde tracing and MEPs analyses showed that numbers of FG-positive sensory neurons and motor neurons as well as MEPs distribution density were higher in the PRP-SAN group than in the PRP or SAN group.
33838005	3	114	theme	autogenous	448:457	arg1	SANs					467:470	SANs	467:470	SANs	467:470	This study explored the efficacy of nerve grafts constructed from chitin biological conduits combined with small autogenous nerves (SANs) and platelet-rich plasma (PRP) for repairing 10-mm sciatic nerve defects in rats.
33838005	3	114	theme	autogenous	448:457	arg1	nerves					459:464	small autogenous nerves	442:464	small autogenous nerves (SANs)	442:471	This study explored the efficacy of nerve grafts constructed from chitin biological conduits combined with small autogenous nerves (SANs) and platelet-rich plasma (PRP) for repairing 10-mm sciatic nerve defects in rats.
33838005	0	115	theme	small	42:46	arg1	nerves					59:64	small autogenous nerves	42:64	small autogenous nerves	42:64	Combining chitin biological conduits with small autogenous nerves and platelet-rich plasma for the repair of sciatic nerve defects in rats.
33838005	8	116	theme	nerve	1485:1489	arg1	density					1497:1503	myelinated nerve fiber density	1474:1503	myelinated nerve fiber density	1474:1503	The PRP-SAN group exhibited better recovery in terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation, compared with the PRP and SAN groups.
33838005	0	117	from	defects	123:129	arg1	rats					134:137	rats	134:137	rats	134:137	Combining chitin biological conduits with small autogenous nerves and platelet-rich plasma for the repair of sciatic nerve defects in rats.
33838005	3	118	theme	nerve	371:375	arg1	grafts					377:382	nerve grafts	371:382	nerve grafts constructed from chitin biological conduits combined with small autogenous nerves (SANs) and platelet-rich plasma (PRP)	371:502	This study explored the efficacy of nerve grafts constructed from chitin biological conduits combined with small autogenous nerves (SANs) and platelet-rich plasma (PRP) for repairing 10-mm sciatic nerve defects in rats.
33838005	8	119	theme	density	1497:1503	arg1	terms					1391:1395	terms	1391:1395	terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation	1391:1586	The PRP-SAN group exhibited better recovery in terms of the sciatic functional index value, composite action potential intensity, myelinated nerve fiber density, myelin sheath thickness, and gastrectomy tissue at 12 weeks after transplantation, compared with the PRP and SAN groups.
33838005	3	120	theme	platelet-rich	477:489	arg1	PRP					499:501	PRP	499:501	PRP	499:501	This study explored the efficacy of nerve grafts constructed from chitin biological conduits combined with small autogenous nerves (SANs) and platelet-rich plasma (PRP) for repairing 10-mm sciatic nerve defects in rats.
33838005	3	120	theme	platelet-rich	477:489	arg1	plasma					491:496	platelet-rich plasma	477:496	platelet-rich plasma (PRP)	477:502	This study explored the efficacy of nerve grafts constructed from chitin biological conduits combined with small autogenous nerves (SANs) and platelet-rich plasma (PRP) for repairing 10-mm sciatic nerve defects in rats.
33838005	2	121	theme	repairing	299:307	arg1	defects					326:332	repairing peripheral nerve defects	299:332	repairing peripheral nerve defects	299:332	Therefore, it is important to explore new methods of repairing peripheral nerve defects.
33838005	7	122	dep	rate	1221:1224	arg1	the					1217:1219	the	1217:1219	the	1217:1219	RESULTS Two weeks after nerve transplantation, the rate and number of regenerated axons in the PRP-SAN group improved compared with those in the PRP, SAN, and Hollow groups.
33838005	7	123	theme	nerve	1194:1198	arg1	transplantation					1200:1214	nerve transplantation	1194:1214	nerve transplantation	1194:1214	RESULTS Two weeks after nerve transplantation, the rate and number of regenerated axons in the PRP-SAN group improved compared with those in the PRP, SAN, and Hollow groups.
33838005	0	124	from	rats	134:137	arg1	repair					99:104	the repair	95:104	the repair of sciatic nerve defects in rats	95:137	Combining chitin biological conduits with small autogenous nerves and platelet-rich plasma for the repair of sciatic nerve defects in rats.
33838005	10	125	theme	nerve	1992:1996	arg1	defects					1998:2004	10-mm sciatic nerve defects	1978:2004	10-mm sciatic nerve defects	1978:2004	CONCLUSIONS Nerve grafts comprising chitin biological conduits combined with SANs and PRP significantly improved the repair of 10-mm sciatic nerve defects in rats and may have therapeutic potential for repairing peripheral nerve defects in future applications.
33838005	9	126	theme	MEPs	1762:1765	arg1	density					1780:1786	MEPs distribution density	1762:1786	numbers of FG-positive sensory neurons and motor neurons as well as MEPs distribution density	1694:1786	The results of FG retrograde tracing and MEPs analyses showed that numbers of FG-positive sensory neurons and motor neurons as well as MEPs distribution density were higher in the PRP-SAN group than in the PRP or SAN group.
33838005	4	127	theme	sciatic	580:586	arg1	defects					594:600	10-mm sciatic nerve defects	574:600	10-mm sciatic nerve defects	574:600	METHODS To prepare 10-mm sciatic nerve defects, SANs were first harvested and PRP was extracted.
32100704	15	0	theme	1.16469T=DSM	1773:1784	arg1	MF30-AT					1757:1763	MF30-AT	1757:1763	MF30-AT (=CGMCC 1.16469T=DSM 106183T)	1757:1793	The type strain is MF30-AT (=CGMCC 1.16469T=DSM 106183T).
32100704	15	0	theme	1.16469T=DSM	1773:1784	arg1	106183T					1786:1792	=CGMCC 1.16469T=DSM 106183T	1766:1792	=CGMCC 1.16469T=DSM 106183T	1766:1792	The type strain is MF30-AT (=CGMCC 1.16469T=DSM 106183T).
32100704	12	1	theme	nucleotide	1340:1349	arg1	identity					1351:1358	average nucleotide identity	1332:1358	average nucleotide identity	1332:1358	The average nucleotide identity and digital DNA-DNA relatedness values of the two strains with all available genomes of the genus Agromyces were far below the respective thresholds of 95 and 70 %, respectively.
32100704	11	2	theme	mol	1308:1310	arg1	%					1311:1311	69.7 mol%	1303:1311	69.7 mol%	1303:1311	The G+C contents of the DNA of strains MF30-AT and MF845 were 69.8 mol% and 69.7 mol%, respectively.
32100704	13	3	theme	Agromyces	1665:1673	arg1	strains					1588:1594	strains MF30-AT and MF845	1588:1612	strains MF30-AT and MF845	1588:1612	All genotypic and phenotypic data indicated that strains MF30-AT and MF845 should be classified as novel members of the genus Agromyces, for which the name Agromyces badenianii sp.
32100704	13	3	theme	Agromyces	1665:1673	arg1	members					1644:1650	novel members	1638:1650	novel members	1638:1650	All genotypic and phenotypic data indicated that strains MF30-AT and MF845 should be classified as novel members of the genus Agromyces, for which the name Agromyces badenianii sp.
32100704	10	4	from	lipids	1039:1044	arg1	MF30-AT					1056:1062	strain MF30-AT	1049:1062	strain MF30-AT	1049:1062	The major polar lipids in strain MF30-AT were diphosphatidylglycerol, phosphatidylglycerol and a glycolipid, while the peptidoglycan contained alanine, glutamic acid, glycine and 2,4-diaminobutyric acid.
32100704	2	5	theme	plateau	254:260	arg1	pika					262:265	plateau pika	254:265	plateau pika collected from the Qinghai-Tibet Plateau	254:306	Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains, designated MF30-AT and MF845, were isolated from the intestinal contents of plateau pika collected from the Qinghai-Tibet Plateau.
32100704	13	6	dep	strains	1588:1594	arg1	strains					1588:1594	strains MF30-AT and MF845	1588:1612	strains MF30-AT and MF845	1588:1612	All genotypic and phenotypic data indicated that strains MF30-AT and MF845 should be classified as novel members of the genus Agromyces, for which the name Agromyces badenianii sp.
32100704	13	6	dep	strains	1588:1594	arg1	MF845					1608:1612	MF845	1608:1612	MF845	1608:1612	All genotypic and phenotypic data indicated that strains MF30-AT and MF845 should be classified as novel members of the genus Agromyces, for which the name Agromyces badenianii sp.
32100704	13	6	dep	strains	1588:1594	arg1	MF30-AT					1596:1602	MF30-AT	1596:1602	MF30-AT	1596:1602	All genotypic and phenotypic data indicated that strains MF30-AT and MF845 should be classified as novel members of the genus Agromyces, for which the name Agromyces badenianii sp.
32100704	11	7	theme	DNA	1251:1253	arg1	contents					1235:1242	The G+C contents	1227:1242	The G+C contents of the DNA of strains MF30-AT and MF845	1227:1282	The G+C contents of the DNA of strains MF30-AT and MF845 were 69.8 mol% and 69.7 mol%, respectively.
32100704	11	7	theme	DNA	1251:1253	arg1	%					1297:1297	69.8 mol%	1289:1297	69.8 mol%	1289:1297	The G+C contents of the DNA of strains MF30-AT and MF845 were 69.8 mol% and 69.7 mol%, respectively.
32100704	7	8	theme	β-glucosidase	759:771	arg1	Production					745:754	Production	745:754	Production of β-glucosidase by these strains	745:788	Production of β-glucosidase by these strains was negative.
32100704	4	9	theme	rRNA	412:415	arg1	sequences					422:430	The 16S rRNA gene sequences	404:430	The 16S rRNA gene sequences of the isolates	404:446	The 16S rRNA gene sequences of the isolates had highest similarities of 98.5 and 98.4 % to Agromyces fucosus, respectively.
32100704	9	10	theme	Strain	898:903	arg1	MF30-AT					905:911	Strain MF30-AT	898:911	Strain MF30-AT	898:911	Strain MF30-AT contained galactose, rhamnose and ribose as cell wall sugars and MK-12 and MK-11 as predominant menaquinones.
32100704	12	11	theme	Agromyces	1458:1466	arg1	genomes					1437:1443	all available genomes	1423:1443	all available genomes of the genus Agromyces	1423:1466	The average nucleotide identity and digital DNA-DNA relatedness values of the two strains with all available genomes of the genus Agromyces were far below the respective thresholds of 95 and 70 %, respectively.
32100704	11	12	theme	strains	1258:1264	arg1	DNA					1251:1253	the DNA	1247:1253	the DNA of strains MF30-AT and MF845	1247:1282	The G+C contents of the DNA of strains MF30-AT and MF845 were 69.8 mol% and 69.7 mol%, respectively.
32100704	5	13	theme	other	625:629	arg1	species					631:637	other species	625:637	other species	625:637	In the 16S rRNA gene and polygenetic trees, strains MF30-AT and MF845 were clearly distinct from other species.
32100704	4	14	dep	Agromyces	495:503	arg1	fucosus					505:511	Agromyces fucosus	495:511	Agromyces fucosus	495:511	The 16S rRNA gene sequences of the isolates had highest similarities of 98.5 and 98.4 % to Agromyces fucosus, respectively.
32100704	5	15	from	trees	565:569	arg1	distinct					611:618	distinct	611:618	distinct	611:618	In the 16S rRNA gene and polygenetic trees, strains MF30-AT and MF845 were clearly distinct from other species.
32100704	3	16	theme	Optimal	309:315	arg1	growth					317:322	Optimal growth	309:322	Optimal growth of these two strains	309:343	Optimal growth of these two strains was observed under aerobic conditions at pH 7.0 and 28 °C.
32100704	10	17	theme	glutamic	1175:1182	arg1	acid					1184:1187	glutamic acid	1175:1187	glutamic acid	1175:1187	The major polar lipids in strain MF30-AT were diphosphatidylglycerol, phosphatidylglycerol and a glycolipid, while the peptidoglycan contained alanine, glutamic acid, glycine and 2,4-diaminobutyric acid.
32100704	12	18	theme	strains	1410:1416	arg1	values					1392:1397	The average nucleotide identity and digital DNA-DNA relatedness values	1328:1397	The average nucleotide identity and digital DNA-DNA relatedness values of the two strains with all available genomes of the genus Agromyces	1328:1466	The average nucleotide identity and digital DNA-DNA relatedness values of the two strains with all available genomes of the genus Agromyces were far below the respective thresholds of 95 and 70 %, respectively.
32100704	4	19	theme	98.5	476:479	arg1	%					490:490	98.5 and 98.4 %	476:490	%	490:490	The 16S rRNA gene sequences of the isolates had highest similarities of 98.5 and 98.4 % to Agromyces fucosus, respectively.
32100704	9	20	theme	wall	962:965	arg1	rhamnose					934:941	rhamnose	934:941	rhamnose	934:941	Strain MF30-AT contained galactose, rhamnose and ribose as cell wall sugars and MK-12 and MK-11 as predominant menaquinones.
32100704	9	20	theme	wall	962:965	arg1	sugars					967:972	cell wall sugars and MK-12 and MK-11	957:992	sugars	967:972	Strain MF30-AT contained galactose, rhamnose and ribose as cell wall sugars and MK-12 and MK-11 as predominant menaquinones.
32100704	9	20	theme	wall	962:965	arg1	galactose					923:931	galactose	923:931	galactose	923:931	Strain MF30-AT contained galactose, rhamnose and ribose as cell wall sugars and MK-12 and MK-11 as predominant menaquinones.
32100704	9	20	theme	wall	962:965	arg1	ribose					947:952	ribose	947:952	ribose	947:952	Strain MF30-AT contained galactose, rhamnose and ribose as cell wall sugars and MK-12 and MK-11 as predominant menaquinones.
32100704	5	21	theme	polygenetic	553:563	arg1	trees					565:569	polygenetic trees	553:569	polygenetic trees	553:569	In the 16S rRNA gene and polygenetic trees, strains MF30-AT and MF845 were clearly distinct from other species.
32100704	12	22	theme	%	1522:1522	arg1	thresholds					1498:1507	the respective thresholds	1483:1507	the respective thresholds of 95 and 70 %, respectively	1483:1536	The average nucleotide identity and digital DNA-DNA relatedness values of the two strains with all available genomes of the genus Agromyces were far below the respective thresholds of 95 and 70 %, respectively.
32100704	13	23	theme	genotypic	1543:1551	arg1	data					1568:1571	All genotypic and phenotypic data	1539:1571	All genotypic and phenotypic data	1539:1571	All genotypic and phenotypic data indicated that strains MF30-AT and MF845 should be classified as novel members of the genus Agromyces, for which the name Agromyces badenianii sp.
32100704	8	24	theme	fatty	814:818	arg1	anteiso-C15					848:858	anteiso-C15	848:858	anteiso-C15	848:858	The major fatty acids of these strains were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
32100704	8	24	theme	fatty	814:818	arg1	acids					820:824	The major fatty acids	804:824	The major fatty acids of these strains	804:841	The major fatty acids of these strains were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
32100704	13	25	theme	phenotypic	1557:1566	arg1	data					1568:1571	All genotypic and phenotypic data	1539:1571	All genotypic and phenotypic data	1539:1571	All genotypic and phenotypic data indicated that strains MF30-AT and MF845 should be classified as novel members of the genus Agromyces, for which the name Agromyces badenianii sp.
32100704	10	26	theme	polar	1033:1037	arg1	diphosphatidylglycerol					1069:1090	diphosphatidylglycerol	1069:1090	diphosphatidylglycerol	1069:1090	The major polar lipids in strain MF30-AT were diphosphatidylglycerol, phosphatidylglycerol and a glycolipid, while the peptidoglycan contained alanine, glutamic acid, glycine and 2,4-diaminobutyric acid.
32100704	10	26	theme	polar	1033:1037	arg1	lipids					1039:1044	The major polar lipids	1023:1044	The major polar lipids in strain MF30-AT	1023:1062	The major polar lipids in strain MF30-AT were diphosphatidylglycerol, phosphatidylglycerol and a glycolipid, while the peptidoglycan contained alanine, glutamic acid, glycine and 2,4-diaminobutyric acid.
32100704	5	27	theme	16S	535:537	arg1	rRNA					539:542	the 16S rRNA	531:542	the 16S rRNA gene	531:547	In the 16S rRNA gene and polygenetic trees, strains MF30-AT and MF845 were clearly distinct from other species.
32100704	13	28	theme	Agromyces	1695:1703	arg1	sp					1716:1717	the name Agromyces badenianii sp	1686:1717	the name Agromyces badenianii sp	1686:1717	All genotypic and phenotypic data indicated that strains MF30-AT and MF845 should be classified as novel members of the genus Agromyces, for which the name Agromyces badenianii sp.
32100704	8	29	theme	strains	835:841	arg1	anteiso-C15					848:858	anteiso-C15	848:858	anteiso-C15	848:858	The major fatty acids of these strains were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
32100704	8	29	theme	strains	835:841	arg1	acids					820:824	The major fatty acids	804:824	The major fatty acids of these strains	804:841	The major fatty acids of these strains were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
32100704	4	30	theme	98.4	485:488	arg1	%					490:490	98.5 and 98.4 %	476:490	%	490:490	The 16S rRNA gene sequences of the isolates had highest similarities of 98.5 and 98.4 % to Agromyces fucosus, respectively.
32100704	2	31	theme	rod-shaped	148:157	arg1	strains					169:175	Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains	80:175	Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains	80:175	Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains, designated MF30-AT and MF845, were isolated from the intestinal contents of plateau pika collected from the Qinghai-Tibet Plateau.
32100704	4	32	contain	had	448:450	arg1	sequences					422:430	The 16S rRNA gene sequences	404:430	The 16S rRNA gene sequences of the isolates	404:446	The 16S rRNA gene sequences of the isolates had highest similarities of 98.5 and 98.4 % to Agromyces fucosus, respectively.
32100704	4	32	contain	had	448:450	arg2	similarities					460:471	highest similarities	452:471	highest similarities of 98.5 and 98.4 % to Agromyces fucosus	452:511	The 16S rRNA gene sequences of the isolates had highest similarities of 98.5 and 98.4 % to Agromyces fucosus, respectively.
32100704	12	33	theme	digital	1364:1370	arg1	relatedness					1380:1390	digital DNA-DNA relatedness	1364:1390	digital DNA-DNA relatedness	1364:1390	The average nucleotide identity and digital DNA-DNA relatedness values of the two strains with all available genomes of the genus Agromyces were far below the respective thresholds of 95 and 70 %, respectively.
32100704	5	34	from	species	631:637	arg1	distinct					611:618	distinct	611:618	distinct	611:618	In the 16S rRNA gene and polygenetic trees, strains MF30-AT and MF845 were clearly distinct from other species.
32100704	12	35	theme	relatedness	1380:1390	arg1	values					1392:1397	The average nucleotide identity and digital DNA-DNA relatedness values	1328:1397	The average nucleotide identity and digital DNA-DNA relatedness values of the two strains with all available genomes of the genus Agromyces	1328:1466	The average nucleotide identity and digital DNA-DNA relatedness values of the two strains with all available genomes of the genus Agromyces were far below the respective thresholds of 95 and 70 %, respectively.
32100704	0	36	dep	sp	21:22	arg1	Agromyces					0:8	Agromyces	0:8	Agromyces	0:8	Agromyces badenianii sp.
32100704	11	37	theme	69.8	1289:1292	arg1	mol					1294:1296	mol	1294:1296	mol	1294:1296	The G+C contents of the DNA of strains MF30-AT and MF845 were 69.8 mol% and 69.7 mol%, respectively.
32100704	12	38	theme	average	1332:1338	arg1	identity					1351:1358	average nucleotide identity	1332:1358	average nucleotide identity	1332:1358	The average nucleotide identity and digital DNA-DNA relatedness values of the two strains with all available genomes of the genus Agromyces were far below the respective thresholds of 95 and 70 %, respectively.
32100704	5	39	from	distinct	611:618	arg1	gene					544:547	the 16S rRNA gene	531:547	the 16S rRNA gene	531:547	In the 16S rRNA gene and polygenetic trees, strains MF30-AT and MF845 were clearly distinct from other species.
32100704	5	39	from	distinct	611:618	arg1	trees					565:569	polygenetic trees	553:569	polygenetic trees	553:569	In the 16S rRNA gene and polygenetic trees, strains MF30-AT and MF845 were clearly distinct from other species.
32100704	2	40	theme	Gram-stain-positive	93:111	arg1	strains					169:175	Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains	80:175	Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains	80:175	Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains, designated MF30-AT and MF845, were isolated from the intestinal contents of plateau pika collected from the Qinghai-Tibet Plateau.
32100704	12	41	theme	identity	1351:1358	arg1	values					1392:1397	The average nucleotide identity and digital DNA-DNA relatedness values	1328:1397	The average nucleotide identity and digital DNA-DNA relatedness values of the two strains with all available genomes of the genus Agromyces	1328:1466	The average nucleotide identity and digital DNA-DNA relatedness values of the two strains with all available genomes of the genus Agromyces were far below the respective thresholds of 95 and 70 %, respectively.
32100704	11	42	theme	69.7	1303:1306	arg1	mol					1308:1310	mol	1308:1310	mol	1308:1310	The G+C contents of the DNA of strains MF30-AT and MF845 were 69.8 mol% and 69.7 mol%, respectively.
32100704	2	43	theme	aerobic	84:90	arg1	strains					169:175	Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains	80:175	Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains	80:175	Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains, designated MF30-AT and MF845, were isolated from the intestinal contents of plateau pika collected from the Qinghai-Tibet Plateau.
32100704	13	44	theme	genus	1659:1663	arg1	Agromyces					1665:1673	the genus Agromyces	1655:1673	the genus Agromyces	1655:1673	All genotypic and phenotypic data indicated that strains MF30-AT and MF845 should be classified as novel members of the genus Agromyces, for which the name Agromyces badenianii sp.
32100704	2	45	theme	non-motile	133:142	arg1	strains					169:175	Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains	80:175	Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains	80:175	Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains, designated MF30-AT and MF845, were isolated from the intestinal contents of plateau pika collected from the Qinghai-Tibet Plateau.
32100704	2	46	theme	pika	262:265	arg1	contents					242:249	the intestinal contents	227:249	the intestinal contents of plateau pika collected from the Qinghai-Tibet Plateau	227:306	Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains, designated MF30-AT and MF845, were isolated from the intestinal contents of plateau pika collected from the Qinghai-Tibet Plateau.
32100704	11	47	dep	strains	1258:1264	arg1	strains					1258:1264	strains MF30-AT and MF845	1258:1282	strains MF30-AT and MF845	1258:1282	The G+C contents of the DNA of strains MF30-AT and MF845 were 69.8 mol% and 69.7 mol%, respectively.
32100704	11	47	dep	strains	1258:1264	arg1	MF845					1278:1282	MF845	1278:1282	MF845	1278:1282	The G+C contents of the DNA of strains MF30-AT and MF845 were 69.8 mol% and 69.7 mol%, respectively.
32100704	11	47	dep	strains	1258:1264	arg1	MF30-AT					1266:1272	MF30-AT	1266:1272	MF30-AT	1266:1272	The G+C contents of the DNA of strains MF30-AT and MF845 were 69.8 mol% and 69.7 mol%, respectively.
32100704	15	48	theme	=CGMCC	1766:1771	arg1	MF30-AT					1757:1763	MF30-AT	1757:1763	MF30-AT (=CGMCC 1.16469T=DSM 106183T)	1757:1793	The type strain is MF30-AT (=CGMCC 1.16469T=DSM 106183T).
32100704	15	48	theme	=CGMCC	1766:1771	arg1	106183T					1786:1792	=CGMCC 1.16469T=DSM 106183T	1766:1792	=CGMCC 1.16469T=DSM 106183T	1766:1792	The type strain is MF30-AT (=CGMCC 1.16469T=DSM 106183T).
32100704	2	49	theme	catalase-positive	114:130	arg1	strains					169:175	Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains	80:175	Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains	80:175	Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains, designated MF30-AT and MF845, were isolated from the intestinal contents of plateau pika collected from the Qinghai-Tibet Plateau.
32100704	12	50	theme	respective	1487:1496	arg1	thresholds					1498:1507	the respective thresholds	1483:1507	the respective thresholds of 95 and 70 %, respectively	1483:1536	The average nucleotide identity and digital DNA-DNA relatedness values of the two strains with all available genomes of the genus Agromyces were far below the respective thresholds of 95 and 70 %, respectively.
32100704	13	51	theme	novel	1638:1642	arg1	strains					1588:1594	strains MF30-AT and MF845	1588:1612	strains MF30-AT and MF845	1588:1612	All genotypic and phenotypic data indicated that strains MF30-AT and MF845 should be classified as novel members of the genus Agromyces, for which the name Agromyces badenianii sp.
32100704	13	51	theme	novel	1638:1642	arg1	members					1644:1650	novel members	1638:1650	novel members	1638:1650	All genotypic and phenotypic data indicated that strains MF30-AT and MF845 should be classified as novel members of the genus Agromyces, for which the name Agromyces badenianii sp.
32100704	2	52	theme	Qinghai-Tibet	286:298	arg1	Plateau					300:306	the Qinghai-Tibet Plateau	282:306	the Qinghai-Tibet Plateau	282:306	Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains, designated MF30-AT and MF845, were isolated from the intestinal contents of plateau pika collected from the Qinghai-Tibet Plateau.
32100704	4	53	theme	gene	417:420	arg1	sequences					422:430	The 16S rRNA gene sequences	404:430	The 16S rRNA gene sequences of the isolates	404:446	The 16S rRNA gene sequences of the isolates had highest similarities of 98.5 and 98.4 % to Agromyces fucosus, respectively.
32100704	15	54	theme	type	1742:1745	arg1	MF30-AT					1757:1763	MF30-AT	1757:1763	MF30-AT (=CGMCC 1.16469T=DSM 106183T)	1757:1793	The type strain is MF30-AT (=CGMCC 1.16469T=DSM 106183T).
32100704	15	54	theme	type	1742:1745	arg1	strain					1747:1752	The type strain	1738:1752	The type strain	1738:1752	The type strain is MF30-AT (=CGMCC 1.16469T=DSM 106183T).
32100704	9	55	theme	cell	957:960	arg1	rhamnose					934:941	rhamnose	934:941	rhamnose	934:941	Strain MF30-AT contained galactose, rhamnose and ribose as cell wall sugars and MK-12 and MK-11 as predominant menaquinones.
32100704	9	55	theme	cell	957:960	arg1	sugars					967:972	cell wall sugars and MK-12 and MK-11	957:992	sugars	967:972	Strain MF30-AT contained galactose, rhamnose and ribose as cell wall sugars and MK-12 and MK-11 as predominant menaquinones.
32100704	9	55	theme	cell	957:960	arg1	galactose					923:931	galactose	923:931	galactose	923:931	Strain MF30-AT contained galactose, rhamnose and ribose as cell wall sugars and MK-12 and MK-11 as predominant menaquinones.
32100704	9	55	theme	cell	957:960	arg1	ribose					947:952	ribose	947:952	ribose	947:952	Strain MF30-AT contained galactose, rhamnose and ribose as cell wall sugars and MK-12 and MK-11 as predominant menaquinones.
32100704	10	56	contain	contained	1156:1164	arg1	peptidoglycan					1142:1154	the peptidoglycan	1138:1154	the peptidoglycan	1138:1154	The major polar lipids in strain MF30-AT were diphosphatidylglycerol, phosphatidylglycerol and a glycolipid, while the peptidoglycan contained alanine, glutamic acid, glycine and 2,4-diaminobutyric acid.
32100704	10	56	contain	contained	1156:1164	arg2	acid					1184:1187	glutamic acid	1175:1187	glutamic acid	1175:1187	The major polar lipids in strain MF30-AT were diphosphatidylglycerol, phosphatidylglycerol and a glycolipid, while the peptidoglycan contained alanine, glutamic acid, glycine and 2,4-diaminobutyric acid.
32100704	10	56	contain	contained	1156:1164	arg2	alanine					1166:1172	alanine	1166:1172	alanine	1166:1172	The major polar lipids in strain MF30-AT were diphosphatidylglycerol, phosphatidylglycerol and a glycolipid, while the peptidoglycan contained alanine, glutamic acid, glycine and 2,4-diaminobutyric acid.
32100704	10	56	contain	contained	1156:1164	arg2	glycine					1190:1196	glycine	1190:1196	glycine	1190:1196	The major polar lipids in strain MF30-AT were diphosphatidylglycerol, phosphatidylglycerol and a glycolipid, while the peptidoglycan contained alanine, glutamic acid, glycine and 2,4-diaminobutyric acid.
32100704	10	56	contain	contained	1156:1164	arg2	acid					1221:1224	2,4-diaminobutyric acid	1202:1224	2,4-diaminobutyric acid	1202:1224	The major polar lipids in strain MF30-AT were diphosphatidylglycerol, phosphatidylglycerol and a glycolipid, while the peptidoglycan contained alanine, glutamic acid, glycine and 2,4-diaminobutyric acid.
32100704	1	57	dep	Ochotona	59:66	arg1	curzoniae					68:76	Ochotona curzoniae	59:76	Ochotona curzoniae	59:76	nov., isolated from plateau pika (Ochotona curzoniae).
32100704	12	58	with	strains	1410:1416	arg1	genomes					1437:1443	all available genomes	1423:1443	all available genomes of the genus Agromyces	1423:1466	The average nucleotide identity and digital DNA-DNA relatedness values of the two strains with all available genomes of the genus Agromyces were far below the respective thresholds of 95 and 70 %, respectively.
32100704	4	59	theme	16S	408:410	arg1	sequences					422:430	The 16S rRNA gene sequences	404:430	The 16S rRNA gene sequences of the isolates	404:446	The 16S rRNA gene sequences of the isolates had highest similarities of 98.5 and 98.4 % to Agromyces fucosus, respectively.
32100704	2	60	theme	intestinal	231:240	arg1	contents					242:249	the intestinal contents	227:249	the intestinal contents of plateau pika collected from the Qinghai-Tibet Plateau	227:306	Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains, designated MF30-AT and MF845, were isolated from the intestinal contents of plateau pika collected from the Qinghai-Tibet Plateau.
32100704	12	61	theme	genus	1452:1456	arg1	Agromyces					1458:1466	the genus Agromyces	1448:1466	the genus Agromyces	1448:1466	The average nucleotide identity and digital DNA-DNA relatedness values of the two strains with all available genomes of the genus Agromyces were far below the respective thresholds of 95 and 70 %, respectively.
32100704	5	62	from	gene	544:547	arg1	distinct					611:618	distinct	611:618	distinct	611:618	In the 16S rRNA gene and polygenetic trees, strains MF30-AT and MF845 were clearly distinct from other species.
32100704	2	63	attach	isolated	213:220	arg1	contents					242:249	the intestinal contents	227:249	the intestinal contents of plateau pika collected from the Qinghai-Tibet Plateau	227:306	Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains, designated MF30-AT and MF845, were isolated from the intestinal contents of plateau pika collected from the Qinghai-Tibet Plateau.
32100704	2	63	attach	isolated	213:220	arg2	strains					169:175	Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains	80:175	Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains	80:175	Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains, designated MF30-AT and MF845, were isolated from the intestinal contents of plateau pika collected from the Qinghai-Tibet Plateau.
32100704	8	64	dep	anteiso-C15	848:858	arg1	0					862:862	0	862:862	0	862:862	The major fatty acids of these strains were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
32100704	8	64	dep	anteiso-C15	848:858	arg1	0					895:895	0	895:895	0	895:895	The major fatty acids of these strains were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
32100704	8	64	dep	anteiso-C15	848:858	arg1	iso-C16					885:891	iso-C16	885:891	iso-C16	885:891	The major fatty acids of these strains were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
32100704	8	64	dep	anteiso-C15	848:858	arg1	0					879:879	0	879:879	0	879:879	The major fatty acids of these strains were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
32100704	1	65	theme	plateau	45:51	arg1	Ochotona					59:66	Ochotona	59:66	Ochotona	59:66	nov., isolated from plateau pika (Ochotona curzoniae).
32100704	1	65	theme	plateau	45:51	arg1	pika					53:56	plateau pika	45:56	plateau pika (Ochotona curzoniae)	45:77	nov., isolated from plateau pika (Ochotona curzoniae).
32100704	12	66	theme	available	1427:1435	arg1	genomes					1437:1443	all available genomes	1423:1443	all available genomes of the genus Agromyces	1423:1466	The average nucleotide identity and digital DNA-DNA relatedness values of the two strains with all available genomes of the genus Agromyces were far below the respective thresholds of 95 and 70 %, respectively.
32100704	4	67	theme	isolates	439:446	arg1	sequences					422:430	The 16S rRNA gene sequences	404:430	The 16S rRNA gene sequences of the isolates	404:446	The 16S rRNA gene sequences of the isolates had highest similarities of 98.5 and 98.4 % to Agromyces fucosus, respectively.
32100704	10	68	theme	2,4-diaminobutyric	1202:1219	arg1	acid					1221:1224	2,4-diaminobutyric acid	1202:1224	2,4-diaminobutyric acid	1202:1224	The major polar lipids in strain MF30-AT were diphosphatidylglycerol, phosphatidylglycerol and a glycolipid, while the peptidoglycan contained alanine, glutamic acid, glycine and 2,4-diaminobutyric acid.
32100704	3	69	theme	strains	337:343	arg1	growth					317:322	Optimal growth	309:322	Optimal growth of these two strains	309:343	Optimal growth of these two strains was observed under aerobic conditions at pH 7.0 and 28 °C.
32100704	4	70	theme	highest	452:458	arg1	similarities					460:471	highest similarities	452:471	highest similarities of 98.5 and 98.4 % to Agromyces fucosus	452:511	The 16S rRNA gene sequences of the isolates had highest similarities of 98.5 and 98.4 % to Agromyces fucosus, respectively.
32100704	11	71	theme	G+C	1231:1233	arg1	contents					1235:1242	The G+C contents	1227:1242	The G+C contents of the DNA of strains MF30-AT and MF845	1227:1282	The G+C contents of the DNA of strains MF30-AT and MF845 were 69.8 mol% and 69.7 mol%, respectively.
32100704	11	71	theme	G+C	1231:1233	arg1	%					1297:1297	69.8 mol%	1289:1297	69.8 mol%	1289:1297	The G+C contents of the DNA of strains MF30-AT and MF845 were 69.8 mol% and 69.7 mol%, respectively.
32100704	0	72	theme	badenianii	10:19	arg1	sp					21:22	badenianii sp	10:22	badenianii sp	10:22	Agromyces badenianii sp.
32100704	8	73	theme	major	808:812	arg1	anteiso-C15					848:858	anteiso-C15	848:858	anteiso-C15	848:858	The major fatty acids of these strains were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
32100704	8	73	theme	major	808:812	arg1	acids					820:824	The major fatty acids	804:824	The major fatty acids of these strains	804:841	The major fatty acids of these strains were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
32100704	13	74	theme	name	1690:1693	arg1	sp					1716:1717	the name Agromyces badenianii sp	1686:1717	the name Agromyces badenianii sp	1686:1717	All genotypic and phenotypic data indicated that strains MF30-AT and MF845 should be classified as novel members of the genus Agromyces, for which the name Agromyces badenianii sp.
32100704	10	75	theme	major	1027:1031	arg1	diphosphatidylglycerol					1069:1090	diphosphatidylglycerol	1069:1090	diphosphatidylglycerol	1069:1090	The major polar lipids in strain MF30-AT were diphosphatidylglycerol, phosphatidylglycerol and a glycolipid, while the peptidoglycan contained alanine, glutamic acid, glycine and 2,4-diaminobutyric acid.
32100704	10	75	theme	major	1027:1031	arg1	lipids					1039:1044	The major polar lipids	1023:1044	The major polar lipids in strain MF30-AT	1023:1062	The major polar lipids in strain MF30-AT were diphosphatidylglycerol, phosphatidylglycerol and a glycolipid, while the peptidoglycan contained alanine, glutamic acid, glycine and 2,4-diaminobutyric acid.
32100704	5	76	theme	rRNA	539:542	arg1	gene					544:547	the 16S rRNA gene	531:547	the 16S rRNA gene	531:547	In the 16S rRNA gene and polygenetic trees, strains MF30-AT and MF845 were clearly distinct from other species.
32100704	12	77	theme	95	1512:1513	arg1	%					1522:1522	95 and 70 %	1512:1522	%	1522:1522	The average nucleotide identity and digital DNA-DNA relatedness values of the two strains with all available genomes of the genus Agromyces were far below the respective thresholds of 95 and 70 %, respectively.
32100704	3	78	theme	aerobic	364:370	arg1	conditions					372:381	aerobic conditions	364:381	aerobic conditions	364:381	Optimal growth of these two strains was observed under aerobic conditions at pH 7.0 and 28 °C.
32100704	9	79	theme	predominant	997:1007	arg1	rhamnose					934:941	rhamnose	934:941	rhamnose	934:941	Strain MF30-AT contained galactose, rhamnose and ribose as cell wall sugars and MK-12 and MK-11 as predominant menaquinones.
32100704	9	79	theme	predominant	997:1007	arg1	galactose					923:931	galactose	923:931	galactose	923:931	Strain MF30-AT contained galactose, rhamnose and ribose as cell wall sugars and MK-12 and MK-11 as predominant menaquinones.
32100704	9	79	theme	predominant	997:1007	arg1	ribose					947:952	ribose	947:952	ribose	947:952	Strain MF30-AT contained galactose, rhamnose and ribose as cell wall sugars and MK-12 and MK-11 as predominant menaquinones.
32100704	9	79	theme	predominant	997:1007	arg1	menaquinones					1009:1020	predominant menaquinones	997:1020	predominant menaquinones	997:1020	Strain MF30-AT contained galactose, rhamnose and ribose as cell wall sugars and MK-12 and MK-11 as predominant menaquinones.
32100704	12	80	theme	70	1519:1520	arg1	%					1522:1522	95 and 70 %	1512:1522	%	1522:1522	The average nucleotide identity and digital DNA-DNA relatedness values of the two strains with all available genomes of the genus Agromyces were far below the respective thresholds of 95 and 70 %, respectively.
32100704	4	81	theme	%	490:490	arg1	similarities					460:471	highest similarities	452:471	highest similarities of 98.5 and 98.4 % to Agromyces fucosus	452:511	The 16S rRNA gene sequences of the isolates had highest similarities of 98.5 and 98.4 % to Agromyces fucosus, respectively.
32100704	2	82	theme	bacterial	159:167	arg1	strains					169:175	Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains	80:175	Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains	80:175	Two aerobic, Gram-stain-positive, catalase-positive, non-motile and rod-shaped bacterial strains, designated MF30-AT and MF845, were isolated from the intestinal contents of plateau pika collected from the Qinghai-Tibet Plateau.
32100704	10	83	theme	strain	1049:1054	arg1	MF30-AT					1056:1062	strain MF30-AT	1049:1062	strain MF30-AT	1049:1062	The major polar lipids in strain MF30-AT were diphosphatidylglycerol, phosphatidylglycerol and a glycolipid, while the peptidoglycan contained alanine, glutamic acid, glycine and 2,4-diaminobutyric acid.
32100704	5	84	dep	strains	572:578	arg1	MF30-AT					580:586	MF30-AT	580:586	MF30-AT	580:586	In the 16S rRNA gene and polygenetic trees, strains MF30-AT and MF845 were clearly distinct from other species.
32100704	5	84	dep	strains	572:578	arg1	strains					572:578	strains	572:578	strains MF30-AT and MF845	572:596	In the 16S rRNA gene and polygenetic trees, strains MF30-AT and MF845 were clearly distinct from other species.
32100704	5	84	dep	strains	572:578	arg1	MF845					592:596	MF845	592:596	MF845	592:596	In the 16S rRNA gene and polygenetic trees, strains MF30-AT and MF845 were clearly distinct from other species.
32100704	3	85	located	observed	349:356	arg1	°C					400:401	28 °C	397:401	28 °C	397:401	Optimal growth of these two strains was observed under aerobic conditions at pH 7.0 and 28 °C.
32100704	3	85	located	observed	349:356	arg1	pH					386:387	pH 7.0	386:391	pH 7.0	386:391	Optimal growth of these two strains was observed under aerobic conditions at pH 7.0 and 28 °C.
32100704	3	85	located	observed	349:356	arg2	growth					317:322	Optimal growth	309:322	Optimal growth of these two strains	309:343	Optimal growth of these two strains was observed under aerobic conditions at pH 7.0 and 28 °C.
32100704	9	86	contain	contained	913:921	arg2	sugars					967:972	cell wall sugars and MK-12 and MK-11	957:992	sugars	967:972	Strain MF30-AT contained galactose, rhamnose and ribose as cell wall sugars and MK-12 and MK-11 as predominant menaquinones.
32100704	9	86	contain	contained	913:921	arg2	ribose					947:952	ribose	947:952	ribose	947:952	Strain MF30-AT contained galactose, rhamnose and ribose as cell wall sugars and MK-12 and MK-11 as predominant menaquinones.
32100704	9	86	contain	contained	913:921	arg2	menaquinones					1009:1020	predominant menaquinones	997:1020	predominant menaquinones	997:1020	Strain MF30-AT contained galactose, rhamnose and ribose as cell wall sugars and MK-12 and MK-11 as predominant menaquinones.
32100704	9	86	contain	contained	913:921	arg1	MF30-AT					905:911	Strain MF30-AT	898:911	Strain MF30-AT	898:911	Strain MF30-AT contained galactose, rhamnose and ribose as cell wall sugars and MK-12 and MK-11 as predominant menaquinones.
32100704	9	86	contain	contained	913:921	arg2	galactose					923:931	galactose	923:931	galactose	923:931	Strain MF30-AT contained galactose, rhamnose and ribose as cell wall sugars and MK-12 and MK-11 as predominant menaquinones.
32100704	9	86	contain	contained	913:921	arg2	rhamnose					934:941	rhamnose	934:941	rhamnose	934:941	Strain MF30-AT contained galactose, rhamnose and ribose as cell wall sugars and MK-12 and MK-11 as predominant menaquinones.
32100704	12	87	theme	DNA-DNA	1372:1378	arg1	relatedness					1380:1390	digital DNA-DNA relatedness	1364:1390	digital DNA-DNA relatedness	1364:1390	The average nucleotide identity and digital DNA-DNA relatedness values of the two strains with all available genomes of the genus Agromyces were far below the respective thresholds of 95 and 70 %, respectively.
32100704	11	88	theme	mol	1294:1296	arg1	contents					1235:1242	The G+C contents	1227:1242	The G+C contents of the DNA of strains MF30-AT and MF845	1227:1282	The G+C contents of the DNA of strains MF30-AT and MF845 were 69.8 mol% and 69.7 mol%, respectively.
32100704	11	88	theme	mol	1294:1296	arg1	%					1297:1297	69.8 mol%	1289:1297	69.8 mol%	1289:1297	The G+C contents of the DNA of strains MF30-AT and MF845 were 69.8 mol% and 69.7 mol%, respectively.
31829917	11	0	theme	Microbacterium	1731:1744	arg1	sp					1757:1758	Microbacterium wangchenii sp	1731:1758	Microbacterium wangchenii sp	1731:1758	Based on phenotypic data and phylogenetic inference, these two strains represent a novel species of the genus Microbacterium, named here as Microbacterium wangchenii sp.
31829917	4	1	theme	genus	645:649	arg1	Microbacterium					651:664	the genus Microbacterium	641:664	the genus Microbacterium	641:664	The phylogenetic analysis based on 16S rRNA gene sequences revealed that dk512T and dk508 were members of the genus Microbacterium, and most closely related to strains Microbacterium mitrae M4-8T and Microbacterium hatanonis FCC-01T.
31829917	7	2	theme	identity	1004:1011	arg1	%					1145:1145	<95 and <70 %	1133:1145	<95 and <70 %	1133:1145	The average nucleotide identity and digital DNA-DNA hybridization values between strain dk512T and previously characterized Microbacterium species were <95 and <70 %, respectively.
31829917	7	2	theme	identity	1004:1011	arg1	values					1047:1052	The average nucleotide identity and digital DNA-DNA hybridization values	981:1052	The average nucleotide identity and digital DNA-DNA hybridization values between strain dk512T and previously characterized Microbacterium species	981:1126	The average nucleotide identity and digital DNA-DNA hybridization values between strain dk512T and previously characterized Microbacterium species were <95 and <70 %, respectively.
31829917	11	3	theme	phylogenetic	1620:1631	arg1	inference					1633:1641	phylogenetic inference	1620:1641	phylogenetic inference	1620:1641	Based on phenotypic data and phylogenetic inference, these two strains represent a novel species of the genus Microbacterium, named here as Microbacterium wangchenii sp.
31829917	6	4	theme	%	978:978	arg1	content					959:965	a G+C content	953:965	a G+C content of 70.6 mol%	953:978	The genome of type strain dk512T was 3.8 Mb with a G+C content of 70.6 mol%.
31829917	8	5	theme	fatty	1210:1214	arg1	acids					1216:1220	the detected primary cellular fatty acids	1180:1220	the detected primary cellular fatty acids	1180:1220	In strain dk512T, the detected primary cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, the main respiratory quinones were MK-9 (37.9 %) and MK-10 (35.7 %), and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol and three unidentified glycolipids.
31829917	8	5	theme	fatty	1210:1214	arg1	anteiso-C15 					1227:1238	anteiso-C15 	1227:1238	anteiso-C15 	1227:1238	In strain dk512T, the detected primary cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, the main respiratory quinones were MK-9 (37.9 %) and MK-10 (35.7 %), and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol and three unidentified glycolipids.
31829917	11	6	theme	wangchenii	1746:1755	arg1	sp					1757:1758	Microbacterium wangchenii sp	1731:1758	Microbacterium wangchenii sp	1731:1758	Based on phenotypic data and phylogenetic inference, these two strains represent a novel species of the genus Microbacterium, named here as Microbacterium wangchenii sp.
31829917	4	7	theme	Microbacterium	651:664	arg1	members					630:636	members	630:636	members of the genus Microbacterium	630:664	The phylogenetic analysis based on 16S rRNA gene sequences revealed that dk512T and dk508 were members of the genus Microbacterium, and most closely related to strains Microbacterium mitrae M4-8T and Microbacterium hatanonis FCC-01T.
31829917	2	8	dep	Gram-stain-positive	146:164	arg1	aerobic					167:173	aerobic	167:173	aerobic	167:173	Two strains of Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508) were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) collected from the Qinghai-Tibet Plateau, PR China.
31829917	2	8	dep	Gram-stain-positive	146:164	arg1	rod-shaped					207:216	rod-shaped	207:216	rod-shaped	207:216	Two strains of Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508) were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) collected from the Qinghai-Tibet Plateau, PR China.
31829917	2	8	dep	Gram-stain-positive	146:164	arg1	non-motile					195:204	non-motile	195:204	non-motile	195:204	Two strains of Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508) were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) collected from the Qinghai-Tibet Plateau, PR China.
31829917	2	8	dep	Gram-stain-positive	146:164	arg1	non-spore-forming					176:192	non-spore-forming	176:192	non-spore-forming	176:192	Two strains of Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508) were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) collected from the Qinghai-Tibet Plateau, PR China.
31829917	7	9	theme	Microbacterium	1105:1118	arg1	species					1120:1126	previously characterized Microbacterium species	1080:1126	previously characterized Microbacterium species	1080:1126	The average nucleotide identity and digital DNA-DNA hybridization values between strain dk512T and previously characterized Microbacterium species were <95 and <70 %, respectively.
31829917	8	10	theme	polar	1341:1345	arg1	lipids					1347:1352	the polar lipids	1337:1352	the polar lipids	1337:1352	In strain dk512T, the detected primary cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, the main respiratory quinones were MK-9 (37.9 %) and MK-10 (35.7 %), and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol and three unidentified glycolipids.
31829917	7	11	theme	average	985:991	arg1	identity					1004:1011	average nucleotide identity	985:1011	average nucleotide identity	985:1011	The average nucleotide identity and digital DNA-DNA hybridization values between strain dk512T and previously characterized Microbacterium species were <95 and <70 %, respectively.
31829917	5	12	theme	1.0 	886:889	arg1	%					890:890	%	890:890	%	890:890	The strains grew optimally on brain-heart infusion (BHI) agar with 5.0 % (v/v) sheep blood at 30 °C, pH 7.0 and with 1.0 % (w/v) NaCl.
31829917	9	13	theme	cell-wall	1454:1462	arg1	rhamnose					1476:1483	rhamnose	1476:1483	rhamnose	1476:1483	The major cell-wall sugars were rhamnose, ribose and galactose.
31829917	9	13	theme	cell-wall	1454:1462	arg1	sugars					1464:1469	The major cell-wall sugars	1444:1469	The major cell-wall sugars	1444:1469	The major cell-wall sugars were rhamnose, ribose and galactose.
31829917	3	14	dep	K-1T	486:489	arg1	similarity					508:517	98.0 and 97.9 % similarity	492:517	98.0 and 97.9 % similarity	492:517	The 16S rRNA gene sequences of the strains showed the highest identity to Microbacterium saccharophilum K-1T (98.0 and 97.9 % similarity, respectively).
31829917	12	15	theme	type	1797:1800	arg1	49313T					1842:1847	=CGMCC 1.16590T=JCM 33494T=KCTC 49313T	1810:1847	=CGMCC 1.16590T=JCM 33494T=KCTC 49313T	1810:1847	nov, where dk512T is designated the type strain (=CGMCC 1.16590T=JCM 33494T=KCTC 49313T).
31829917	12	15	theme	type	1797:1800	arg1	strain					1802:1807	the type strain	1793:1807	the type strain (=CGMCC 1.16590T=JCM 33494T=KCTC 49313T)	1793:1848	nov, where dk512T is designated the type strain (=CGMCC 1.16590T=JCM 33494T=KCTC 49313T).
31829917	7	16	theme	nucleotide	993:1002	arg1	identity					1004:1011	average nucleotide identity	985:1011	average nucleotide identity	985:1011	The average nucleotide identity and digital DNA-DNA hybridization values between strain dk512T and previously characterized Microbacterium species were <95 and <70 %, respectively.
31829917	5	17	theme	%	890:890	arg1	NaCl					898:901	1.0 % (w/v) NaCl	886:901	1.0 % (w/v) NaCl	886:901	The strains grew optimally on brain-heart infusion (BHI) agar with 5.0 % (v/v) sheep blood at 30 °C, pH 7.0 and with 1.0 % (w/v) NaCl.
31829917	10	18	theme	cell-wall	1566:1574	arg1	peptidoglycan					1576:1588	the cell-wall peptidoglycan	1562:1588	the cell-wall peptidoglycan	1562:1588	Alanine, glutamic acid, glycine and ornithine were in the cell-wall peptidoglycan.
31829917	1	19	attach	isolated	36:43	arg1	faeces					50:55	faeces	50:55	faeces of Tibetan gazelles (Procapra picticaudata)	50:99	nov., isolated from faeces of Tibetan gazelles (Procapra picticaudata) on the Qinghai-Tibet Plateau.
31829917	1	19	attach	isolated	36:43	arg2	nov.					30:33	nov.	30:33	nov.	30:33	nov., isolated from faeces of Tibetan gazelles (Procapra picticaudata) on the Qinghai-Tibet Plateau.
31829917	8	20	theme	detected	1184:1191	arg1	acids					1216:1220	the detected primary cellular fatty acids	1180:1220	the detected primary cellular fatty acids	1180:1220	In strain dk512T, the detected primary cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, the main respiratory quinones were MK-9 (37.9 %) and MK-10 (35.7 %), and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol and three unidentified glycolipids.
31829917	8	20	theme	detected	1184:1191	arg1	anteiso-C15 					1227:1238	anteiso-C15 	1227:1238	anteiso-C15 	1227:1238	In strain dk512T, the detected primary cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, the main respiratory quinones were MK-9 (37.9 %) and MK-10 (35.7 %), and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol and three unidentified glycolipids.
31829917	3	21	dep	Microbacterium	456:469	arg1	saccharophilum					471:484	saccharophilum	471:484	saccharophilum	471:484	The 16S rRNA gene sequences of the strains showed the highest identity to Microbacterium saccharophilum K-1T (98.0 and 97.9 % similarity, respectively).
31829917	5	22	theme	5.0 	836:839	arg1	%					840:840	%	840:840	%	840:840	The strains grew optimally on brain-heart infusion (BHI) agar with 5.0 % (v/v) sheep blood at 30 °C, pH 7.0 and with 1.0 % (w/v) NaCl.
31829917	7	23	theme	DNA-DNA	1025:1031	arg1	hybridization					1033:1045	digital DNA-DNA hybridization	1017:1045	digital DNA-DNA hybridization	1017:1045	The average nucleotide identity and digital DNA-DNA hybridization values between strain dk512T and previously characterized Microbacterium species were <95 and <70 %, respectively.
31829917	7	24	theme	hybridization	1033:1045	arg1	%					1145:1145	<95 and <70 %	1133:1145	<95 and <70 %	1133:1145	The average nucleotide identity and digital DNA-DNA hybridization values between strain dk512T and previously characterized Microbacterium species were <95 and <70 %, respectively.
31829917	7	24	theme	hybridization	1033:1045	arg1	values					1047:1052	The average nucleotide identity and digital DNA-DNA hybridization values	981:1052	The average nucleotide identity and digital DNA-DNA hybridization values between strain dk512T and previously characterized Microbacterium species	981:1126	The average nucleotide identity and digital DNA-DNA hybridization values between strain dk512T and previously characterized Microbacterium species were <95 and <70 %, respectively.
31829917	5	25	from	30 °C	863:867	arg1	agar					826:829	brain-heart infusion (BHI) agar	799:829	brain-heart infusion (BHI) agar with 5.0 % (v/v) sheep blood at 30 °C, pH 7.0 and with 1.0 % (w/v) NaCl	799:901	The strains grew optimally on brain-heart infusion (BHI) agar with 5.0 % (v/v) sheep blood at 30 °C, pH 7.0 and with 1.0 % (w/v) NaCl.
31829917	1	26	theme	gazelles	68:75	arg1	faeces					50:55	faeces	50:55	faeces of Tibetan gazelles (Procapra picticaudata)	50:99	nov., isolated from faeces of Tibetan gazelles (Procapra picticaudata) on the Qinghai-Tibet Plateau.
31829917	6	27	theme	70.6 mol	970:977	arg1	%					978:978	70.6 mol%	970:978	70.6 mol%	970:978	The genome of type strain dk512T was 3.8 Mb with a G+C content of 70.6 mol%.
31829917	2	28	theme	PR	372:373	arg1	Plateau					363:369	the Qinghai-Tibet Plateau	345:369	the Qinghai-Tibet Plateau	345:369	Two strains of Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508) were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) collected from the Qinghai-Tibet Plateau, PR China.
31829917	2	28	theme	PR	372:373	arg1	China					375:379	PR China	372:379	PR China	372:379	Two strains of Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508) were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) collected from the Qinghai-Tibet Plateau, PR China.
31829917	0	29	theme	wangchenii	15:24	arg1	sp					26:27	Microbacterium wangchenii sp	0:27	Microbacterium wangchenii sp.	0:28	Microbacterium wangchenii sp.
31829917	11	30	theme	Microbacterium	1701:1714	arg1	species					1680:1686	a novel species	1672:1686	a novel species	1672:1686	Based on phenotypic data and phylogenetic inference, these two strains represent a novel species of the genus Microbacterium, named here as Microbacterium wangchenii sp.
31829917	12	31	theme	1.16590T=JCM	1817:1828	arg1	49313T					1842:1847	=CGMCC 1.16590T=JCM 33494T=KCTC 49313T	1810:1847	=CGMCC 1.16590T=JCM 33494T=KCTC 49313T	1810:1847	nov, where dk512T is designated the type strain (=CGMCC 1.16590T=JCM 33494T=KCTC 49313T).
31829917	12	31	theme	1.16590T=JCM	1817:1828	arg1	strain					1802:1807	the type strain	1793:1807	the type strain (=CGMCC 1.16590T=JCM 33494T=KCTC 49313T)	1793:1848	nov, where dk512T is designated the type strain (=CGMCC 1.16590T=JCM 33494T=KCTC 49313T).
31829917	2	32	theme	gazelle	298:304	arg1	picticaudata					316:327	picticaudata	316:327	picticaudata	316:327	Two strains of Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508) were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) collected from the Qinghai-Tibet Plateau, PR China.
31829917	2	32	theme	gazelle	298:304	arg1	faeces					280:285	the faeces	276:285	the faeces of Tibetan gazelle (Procapra picticaudata) collected from the Qinghai-Tibet Plateau, PR China	276:379	Two strains of Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508) were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) collected from the Qinghai-Tibet Plateau, PR China.
31829917	3	33	theme	gene	395:398	arg1	sequences					400:408	The 16S rRNA gene sequences	382:408	The 16S rRNA gene sequences of the strains	382:423	The 16S rRNA gene sequences of the strains showed the highest identity to Microbacterium saccharophilum K-1T (98.0 and 97.9 % similarity, respectively).
31829917	0	34	theme	Microbacterium	0:13	arg1	sp					26:27	Microbacterium wangchenii sp	0:27	Microbacterium wangchenii sp.	0:28	Microbacterium wangchenii sp.
31829917	2	35	theme	Tibetan	290:296	arg1	gazelle					298:304	Tibetan gazelle	290:304	Tibetan gazelle	290:304	Two strains of Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508) were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) collected from the Qinghai-Tibet Plateau, PR China.
31829917	5	36	theme	%	840:840	arg1	blood					854:858	5.0 % (v/v) sheep blood	836:858	5.0 % (v/v) sheep blood	836:858	The strains grew optimally on brain-heart infusion (BHI) agar with 5.0 % (v/v) sheep blood at 30 °C, pH 7.0 and with 1.0 % (w/v) NaCl.
31829917	8	37	theme	cellular	1201:1208	arg1	acids					1216:1220	the detected primary cellular fatty acids	1180:1220	the detected primary cellular fatty acids	1180:1220	In strain dk512T, the detected primary cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, the main respiratory quinones were MK-9 (37.9 %) and MK-10 (35.7 %), and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol and three unidentified glycolipids.
31829917	8	37	theme	cellular	1201:1208	arg1	anteiso-C15 					1227:1238	anteiso-C15 	1227:1238	anteiso-C15 	1227:1238	In strain dk512T, the detected primary cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, the main respiratory quinones were MK-9 (37.9 %) and MK-10 (35.7 %), and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol and three unidentified glycolipids.
31829917	8	38	theme	primary	1193:1199	arg1	acids					1216:1220	the detected primary cellular fatty acids	1180:1220	the detected primary cellular fatty acids	1180:1220	In strain dk512T, the detected primary cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, the main respiratory quinones were MK-9 (37.9 %) and MK-10 (35.7 %), and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol and three unidentified glycolipids.
31829917	8	38	theme	primary	1193:1199	arg1	anteiso-C15 					1227:1238	anteiso-C15 	1227:1238	anteiso-C15 	1227:1238	In strain dk512T, the detected primary cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, the main respiratory quinones were MK-9 (37.9 %) and MK-10 (35.7 %), and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol and three unidentified glycolipids.
31829917	9	39	theme	major	1448:1452	arg1	rhamnose					1476:1483	rhamnose	1476:1483	rhamnose	1476:1483	The major cell-wall sugars were rhamnose, ribose and galactose.
31829917	9	39	theme	major	1448:1452	arg1	sugars					1464:1469	The major cell-wall sugars	1444:1469	The major cell-wall sugars	1444:1469	The major cell-wall sugars were rhamnose, ribose and galactose.
31829917	8	40	theme	respiratory	1273:1283	arg1	quinones					1285:1292	the main respiratory quinones	1264:1292	the main respiratory quinones	1264:1292	In strain dk512T, the detected primary cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, the main respiratory quinones were MK-9 (37.9 %) and MK-10 (35.7 %), and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol and three unidentified glycolipids.
31829917	8	40	theme	respiratory	1273:1283	arg1	MK-9					1299:1302	MK-9	1299:1302	MK-9	1299:1302	In strain dk512T, the detected primary cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, the main respiratory quinones were MK-9 (37.9 %) and MK-10 (35.7 %), and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol and three unidentified glycolipids.
31829917	2	41	dep	bacteria	218:225	arg1	dk508					250:254	dk508	250:254	dk508	250:254	Two strains of Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508) were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) collected from the Qinghai-Tibet Plateau, PR China.
31829917	2	41	dep	bacteria	218:225	arg1	bacteria					218:225	Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria	146:225	Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508)	146:255	Two strains of Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508) were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) collected from the Qinghai-Tibet Plateau, PR China.
31829917	2	41	dep	bacteria	218:225	arg1	dk512T					239:244	designated dk512T	228:244	designated dk512T	228:244	Two strains of Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508) were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) collected from the Qinghai-Tibet Plateau, PR China.
31829917	5	42	theme	brain-heart	799:809	arg1	agar					826:829	brain-heart infusion (BHI) agar	799:829	brain-heart infusion (BHI) agar with 5.0 % (v/v) sheep blood at 30 °C, pH 7.0 and with 1.0 % (w/v) NaCl	799:901	The strains grew optimally on brain-heart infusion (BHI) agar with 5.0 % (v/v) sheep blood at 30 °C, pH 7.0 and with 1.0 % (w/v) NaCl.
31829917	8	43	dep	anteiso-C15 	1227:1238	arg1	 0					1260:1261	 0	1260:1261	 0	1260:1261	In strain dk512T, the detected primary cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, the main respiratory quinones were MK-9 (37.9 %) and MK-10 (35.7 %), and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol and three unidentified glycolipids.
31829917	8	43	dep	anteiso-C15 	1227:1238	arg1	anteiso-C17 					1247:1258	anteiso-C17 	1247:1258	anteiso-C17 	1247:1258	In strain dk512T, the detected primary cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, the main respiratory quinones were MK-9 (37.9 %) and MK-10 (35.7 %), and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol and three unidentified glycolipids.
31829917	8	43	dep	anteiso-C15 	1227:1238	arg1	 0					1240:1241	 0	1240:1241	 0	1240:1241	In strain dk512T, the detected primary cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, the main respiratory quinones were MK-9 (37.9 %) and MK-10 (35.7 %), and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol and three unidentified glycolipids.
31829917	8	44	theme	strain	1165:1170	arg1	dk512T					1172:1177	strain dk512T	1165:1177	strain dk512T	1165:1177	In strain dk512T, the detected primary cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, the main respiratory quinones were MK-9 (37.9 %) and MK-10 (35.7 %), and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol and three unidentified glycolipids.
31829917	8	45	theme	main	1268:1271	arg1	quinones					1285:1292	the main respiratory quinones	1264:1292	the main respiratory quinones	1264:1292	In strain dk512T, the detected primary cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, the main respiratory quinones were MK-9 (37.9 %) and MK-10 (35.7 %), and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol and three unidentified glycolipids.
31829917	8	45	theme	main	1268:1271	arg1	MK-9					1299:1302	MK-9	1299:1302	MK-9	1299:1302	In strain dk512T, the detected primary cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, the main respiratory quinones were MK-9 (37.9 %) and MK-10 (35.7 %), and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol and three unidentified glycolipids.
31829917	5	46	theme	sheep	848:852	arg1	blood					854:858	5.0 % (v/v) sheep blood	836:858	5.0 % (v/v) sheep blood	836:858	The strains grew optimally on brain-heart infusion (BHI) agar with 5.0 % (v/v) sheep blood at 30 °C, pH 7.0 and with 1.0 % (w/v) NaCl.
31829917	3	47	theme	highest	436:442	arg1	identity					444:451	the highest identity	432:451	the highest identity to Microbacterium saccharophilum K-1T (98.0 and 97.9 % similarity, respectively)	432:532	The 16S rRNA gene sequences of the strains showed the highest identity to Microbacterium saccharophilum K-1T (98.0 and 97.9 % similarity, respectively).
31829917	3	48	theme	16S	386:388	arg1	sequences					400:408	The 16S rRNA gene sequences	382:408	The 16S rRNA gene sequences of the strains	382:423	The 16S rRNA gene sequences of the strains showed the highest identity to Microbacterium saccharophilum K-1T (98.0 and 97.9 % similarity, respectively).
31829917	8	49	theme	unidentified	1418:1429	arg1	glycolipids					1431:1441	three unidentified glycolipids	1412:1441	three unidentified glycolipids	1412:1441	In strain dk512T, the detected primary cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, the main respiratory quinones were MK-9 (37.9 %) and MK-10 (35.7 %), and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol and three unidentified glycolipids.
31829917	5	50	theme	BHI	821:823	arg1	agar					826:829	brain-heart infusion (BHI) agar	799:829	brain-heart infusion (BHI) agar with 5.0 % (v/v) sheep blood at 30 °C, pH 7.0 and with 1.0 % (w/v) NaCl	799:901	The strains grew optimally on brain-heart infusion (BHI) agar with 5.0 % (v/v) sheep blood at 30 °C, pH 7.0 and with 1.0 % (w/v) NaCl.
31829917	2	51	theme	designated	228:237	arg1	bacteria					218:225	Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria	146:225	Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508)	146:255	Two strains of Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508) were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) collected from the Qinghai-Tibet Plateau, PR China.
31829917	2	51	theme	designated	228:237	arg1	dk512T					239:244	designated dk512T	228:244	designated dk512T	228:244	Two strains of Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508) were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) collected from the Qinghai-Tibet Plateau, PR China.
31829917	3	52	theme	strains	417:423	arg1	sequences					400:408	The 16S rRNA gene sequences	382:408	The 16S rRNA gene sequences of the strains	382:423	The 16S rRNA gene sequences of the strains showed the highest identity to Microbacterium saccharophilum K-1T (98.0 and 97.9 % similarity, respectively).
31829917	3	53	theme	rRNA	390:393	arg1	sequences					400:408	The 16S rRNA gene sequences	382:408	The 16S rRNA gene sequences of the strains	382:423	The 16S rRNA gene sequences of the strains showed the highest identity to Microbacterium saccharophilum K-1T (98.0 and 97.9 % similarity, respectively).
31829917	4	54	theme	phylogenetic	539:550	arg1	analysis					552:559	The phylogenetic analysis	535:559	The phylogenetic analysis based on 16S rRNA gene sequences	535:592	The phylogenetic analysis based on 16S rRNA gene sequences revealed that dk512T and dk508 were members of the genus Microbacterium, and most closely related to strains Microbacterium mitrae M4-8T and Microbacterium hatanonis FCC-01T.
31829917	5	55	dep	%	840:840	arg1	v/v					843:845	v/v	843:845	v/v	843:845	The strains grew optimally on brain-heart infusion (BHI) agar with 5.0 % (v/v) sheep blood at 30 °C, pH 7.0 and with 1.0 % (w/v) NaCl.
31829917	6	56	theme	G+C	955:957	arg1	content					959:965	a G+C content	953:965	a G+C content of 70.6 mol%	953:978	The genome of type strain dk512T was 3.8 Mb with a G+C content of 70.6 mol%.
31829917	7	57	theme	digital	1017:1023	arg1	hybridization					1033:1045	digital DNA-DNA hybridization	1017:1045	digital DNA-DNA hybridization	1017:1045	The average nucleotide identity and digital DNA-DNA hybridization values between strain dk512T and previously characterized Microbacterium species were <95 and <70 %, respectively.
31829917	11	58	theme	genus	1695:1699	arg1	Microbacterium					1701:1714	the genus Microbacterium	1691:1714	the genus Microbacterium	1691:1714	Based on phenotypic data and phylogenetic inference, these two strains represent a novel species of the genus Microbacterium, named here as Microbacterium wangchenii sp.
31829917	2	59	theme	Gram-stain-positive	146:164	arg1	dk508					250:254	dk508	250:254	dk508	250:254	Two strains of Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508) were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) collected from the Qinghai-Tibet Plateau, PR China.
31829917	2	59	theme	Gram-stain-positive	146:164	arg1	bacteria					218:225	Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria	146:225	Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508)	146:255	Two strains of Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508) were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) collected from the Qinghai-Tibet Plateau, PR China.
31829917	2	59	theme	Gram-stain-positive	146:164	arg1	dk512T					239:244	designated dk512T	228:244	designated dk512T	228:244	Two strains of Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508) were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) collected from the Qinghai-Tibet Plateau, PR China.
31829917	2	60	theme	bacteria	218:225	arg1	strains					135:141	Two strains	131:141	Two strains of Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508)	131:255	Two strains of Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508) were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) collected from the Qinghai-Tibet Plateau, PR China.
31829917	3	61	theme	Microbacterium	456:469	arg1	K-1T					486:489	Microbacterium saccharophilum K-1T	456:489	Microbacterium saccharophilum K-1T (98.0 and 97.9 % similarity, respectively)	456:532	The 16S rRNA gene sequences of the strains showed the highest identity to Microbacterium saccharophilum K-1T (98.0 and 97.9 % similarity, respectively).
31829917	4	62	theme	16S	570:572	arg1	sequences					584:592	16S rRNA gene sequences	570:592	16S rRNA gene sequences	570:592	The phylogenetic analysis based on 16S rRNA gene sequences revealed that dk512T and dk508 were members of the genus Microbacterium, and most closely related to strains Microbacterium mitrae M4-8T and Microbacterium hatanonis FCC-01T.
31829917	7	63	theme	characterized	1091:1103	arg1	species					1120:1126	previously characterized Microbacterium species	1080:1126	previously characterized Microbacterium species	1080:1126	The average nucleotide identity and digital DNA-DNA hybridization values between strain dk512T and previously characterized Microbacterium species were <95 and <70 %, respectively.
31829917	3	64	dep	%	506:506	arg1	97.9 					501:505	97.9 	501:505	97.9 	501:505	The 16S rRNA gene sequences of the strains showed the highest identity to Microbacterium saccharophilum K-1T (98.0 and 97.9 % similarity, respectively).
31829917	3	64	dep	%	506:506	arg1	98.0					492:495	98.0	492:495	98.0	492:495	The 16S rRNA gene sequences of the strains showed the highest identity to Microbacterium saccharophilum K-1T (98.0 and 97.9 % similarity, respectively).
31829917	12	65	theme	33494T=KCTC	1830:1840	arg1	49313T					1842:1847	=CGMCC 1.16590T=JCM 33494T=KCTC 49313T	1810:1847	=CGMCC 1.16590T=JCM 33494T=KCTC 49313T	1810:1847	nov, where dk512T is designated the type strain (=CGMCC 1.16590T=JCM 33494T=KCTC 49313T).
31829917	12	65	theme	33494T=KCTC	1830:1840	arg1	strain					1802:1807	the type strain	1793:1807	the type strain (=CGMCC 1.16590T=JCM 33494T=KCTC 49313T)	1793:1848	nov, where dk512T is designated the type strain (=CGMCC 1.16590T=JCM 33494T=KCTC 49313T).
31829917	2	66	attach	isolated	262:269	arg1	picticaudata					316:327	picticaudata	316:327	picticaudata	316:327	Two strains of Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508) were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) collected from the Qinghai-Tibet Plateau, PR China.
31829917	2	66	attach	isolated	262:269	arg2	strains					135:141	Two strains	131:141	Two strains of Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508)	131:255	Two strains of Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508) were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) collected from the Qinghai-Tibet Plateau, PR China.
31829917	2	66	attach	isolated	262:269	arg1	faeces					280:285	the faeces	276:285	the faeces of Tibetan gazelle (Procapra picticaudata) collected from the Qinghai-Tibet Plateau, PR China	276:379	Two strains of Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508) were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) collected from the Qinghai-Tibet Plateau, PR China.
31829917	5	67	with	agar	826:829	arg1	NaCl					898:901	1.0 % (w/v) NaCl	886:901	1.0 % (w/v) NaCl	886:901	The strains grew optimally on brain-heart infusion (BHI) agar with 5.0 % (v/v) sheep blood at 30 °C, pH 7.0 and with 1.0 % (w/v) NaCl.
31829917	5	67	with	agar	826:829	arg1	blood					854:858	5.0 % (v/v) sheep blood	836:858	5.0 % (v/v) sheep blood	836:858	The strains grew optimally on brain-heart infusion (BHI) agar with 5.0 % (v/v) sheep blood at 30 °C, pH 7.0 and with 1.0 % (w/v) NaCl.
31829917	8	68	from	anteiso-C15 	1227:1238	arg1	dk512T					1172:1177	strain dk512T	1165:1177	strain dk512T	1165:1177	In strain dk512T, the detected primary cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, the main respiratory quinones were MK-9 (37.9 %) and MK-10 (35.7 %), and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol and three unidentified glycolipids.
31829917	6	69	theme	type	918:921	arg1	dk512T					930:935	type strain dk512T	918:935	type strain dk512T	918:935	The genome of type strain dk512T was 3.8 Mb with a G+C content of 70.6 mol%.
31829917	1	70	theme	Qinghai-Tibet	108:120	arg1	Plateau					122:128	the Qinghai-Tibet Plateau	104:128	the Qinghai-Tibet Plateau	104:128	nov., isolated from faeces of Tibetan gazelles (Procapra picticaudata) on the Qinghai-Tibet Plateau.
31829917	4	71	theme	rRNA	574:577	arg1	sequences					584:592	16S rRNA gene sequences	570:592	16S rRNA gene sequences	570:592	The phylogenetic analysis based on 16S rRNA gene sequences revealed that dk512T and dk508 were members of the genus Microbacterium, and most closely related to strains Microbacterium mitrae M4-8T and Microbacterium hatanonis FCC-01T.
31829917	11	72	theme	novel	1674:1678	arg1	species					1680:1686	a novel species	1672:1686	a novel species	1672:1686	Based on phenotypic data and phylogenetic inference, these two strains represent a novel species of the genus Microbacterium, named here as Microbacterium wangchenii sp.
31829917	5	73	dep	%	890:890	arg1	w/v					893:895	w/v	893:895	w/v	893:895	The strains grew optimally on brain-heart infusion (BHI) agar with 5.0 % (v/v) sheep blood at 30 °C, pH 7.0 and with 1.0 % (w/v) NaCl.
31829917	3	74	dep	similarity	508:517	arg1	%					506:506	%	506:506	%	506:506	The 16S rRNA gene sequences of the strains showed the highest identity to Microbacterium saccharophilum K-1T (98.0 and 97.9 % similarity, respectively).
31829917	6	75	theme	dk512T	930:935	arg1	genome					908:913	The genome	904:913	The genome of type strain dk512T	904:935	The genome of type strain dk512T was 3.8 Mb with a G+C content of 70.6 mol%.
31829917	5	76	theme	infusion	811:818	arg1	agar					826:829	brain-heart infusion (BHI) agar	799:829	brain-heart infusion (BHI) agar with 5.0 % (v/v) sheep blood at 30 °C, pH 7.0 and with 1.0 % (w/v) NaCl	799:901	The strains grew optimally on brain-heart infusion (BHI) agar with 5.0 % (v/v) sheep blood at 30 °C, pH 7.0 and with 1.0 % (w/v) NaCl.
31829917	2	77	theme	Qinghai-Tibet	349:361	arg1	Plateau					363:369	the Qinghai-Tibet Plateau	345:369	the Qinghai-Tibet Plateau	345:369	Two strains of Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508) were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) collected from the Qinghai-Tibet Plateau, PR China.
31829917	2	77	theme	Qinghai-Tibet	349:361	arg1	China					375:379	PR China	372:379	PR China	372:379	Two strains of Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508) were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) collected from the Qinghai-Tibet Plateau, PR China.
31829917	10	78	theme	glutamic	1517:1524	arg1	acid					1526:1529	glutamic acid	1517:1529	glutamic acid	1517:1529	Alanine, glutamic acid, glycine and ornithine were in the cell-wall peptidoglycan.
31829917	11	79	theme	phenotypic	1600:1609	arg1	data					1611:1614	phenotypic data	1600:1614	phenotypic data	1600:1614	Based on phenotypic data and phylogenetic inference, these two strains represent a novel species of the genus Microbacterium, named here as Microbacterium wangchenii sp.
31829917	12	80	theme	=CGMCC	1810:1815	arg1	49313T					1842:1847	=CGMCC 1.16590T=JCM 33494T=KCTC 49313T	1810:1847	=CGMCC 1.16590T=JCM 33494T=KCTC 49313T	1810:1847	nov, where dk512T is designated the type strain (=CGMCC 1.16590T=JCM 33494T=KCTC 49313T).
31829917	12	80	theme	=CGMCC	1810:1815	arg1	strain					1802:1807	the type strain	1793:1807	the type strain (=CGMCC 1.16590T=JCM 33494T=KCTC 49313T)	1793:1848	nov, where dk512T is designated the type strain (=CGMCC 1.16590T=JCM 33494T=KCTC 49313T).
31829917	7	81	theme	strain	1062:1067	arg1	dk512T					1069:1074	strain dk512T	1062:1074	strain dk512T	1062:1074	The average nucleotide identity and digital DNA-DNA hybridization values between strain dk512T and previously characterized Microbacterium species were <95 and <70 %, respectively.
31829917	6	82	theme	strain	923:928	arg1	dk512T					930:935	type strain dk512T	918:935	type strain dk512T	918:935	The genome of type strain dk512T was 3.8 Mb with a G+C content of 70.6 mol%.
31829917	4	83	theme	gene	579:582	arg1	sequences					584:592	16S rRNA gene sequences	570:592	16S rRNA gene sequences	570:592	The phylogenetic analysis based on 16S rRNA gene sequences revealed that dk512T and dk508 were members of the genus Microbacterium, and most closely related to strains Microbacterium mitrae M4-8T and Microbacterium hatanonis FCC-01T.
34294292	8	0	theme	microorganisms	1235:1248	arg1	accumulation					1219:1230	the accumulation	1215:1230	the accumulation of microorganisms	1215:1248	Therefore, the manufactured composite electrospun fiber mat can be used not only in biomedical fields but also in various applications that need to prevent the accumulation of microorganisms.
34294292	1	1	theme	biocompatible	193:205	arg1	Cellulose					164:172	Cellulose	164:172	Cellulose	164:172	Cellulose is well known as a biocompatible material or natural reducing material.
34294292	1	1	theme	biocompatible	193:205	arg1	material					207:214	a biocompatible material	191:214	a biocompatible material	191:214	Cellulose is well known as a biocompatible material or natural reducing material.
34294292	0	2	theme	photocatalytic	102:115	arg1	activity					117:124	facile UV-light photocatalytic activity	86:124	facile UV-light photocatalytic activity for anti-microbial functionalization	86:161	In-situ cellulose-framework templates mediated monodispersed silver nanoparticles via facile UV-light photocatalytic activity for anti-microbial functionalization.
34294292	0	3	theme	anti-microbial	130:143	arg1	functionalization					145:161	anti-microbial functionalization	130:161	anti-microbial functionalization	130:161	In-situ cellulose-framework templates mediated monodispersed silver nanoparticles via facile UV-light photocatalytic activity for anti-microbial functionalization.
34294292	4	4	theme	Ag	609:610	arg1	ions					612:615	Ag ions	609:615	Ag ions	609:615	UV-irradiation causes the electrical change of the cellulose-framework, thereby converting Ag ions into Ag particles.
34294292	8	5	theme	composite	1087:1095	arg1	mat					1115:1117	the manufactured composite electrospun fiber mat	1070:1117	the manufactured composite electrospun fiber mat	1070:1117	Therefore, the manufactured composite electrospun fiber mat can be used not only in biomedical fields but also in various applications that need to prevent the accumulation of microorganisms.
34294292	2	6	theme	silver	325:330	arg1	method					291:296	an eco-friendly and facile method	264:296	an eco-friendly and facile method	264:296	In this study, As an eco-friendly and facile method, we prepared monodispersed silver nanoparticles (AgNPs) in cellulose-framework through photocatalytic reaction.
34294292	2	6	theme	silver	325:330	arg1	AgNPs					347:351	AgNPs	347:351	AgNPs	347:351	In this study, As an eco-friendly and facile method, we prepared monodispersed silver nanoparticles (AgNPs) in cellulose-framework through photocatalytic reaction.
34294292	2	6	theme	silver	325:330	arg1	nanoparticles					332:344	monodispersed silver nanoparticles	311:344	monodispersed silver nanoparticles (AgNPs)	311:352	In this study, As an eco-friendly and facile method, we prepared monodispersed silver nanoparticles (AgNPs) in cellulose-framework through photocatalytic reaction.
34294292	7	7	theme	bone	1040:1043	arg1	regeneration					1045:1056	bone regeneration	1040:1056	bone regeneration	1040:1056	In addition, it was suggested that the gradual release of simvastatin contained in the nanofibers and excellent mineralization would be easy to apply to bone regeneration.
34294292	2	8	theme	monodispersed	311:323	arg1	method					291:296	an eco-friendly and facile method	264:296	an eco-friendly and facile method	264:296	In this study, As an eco-friendly and facile method, we prepared monodispersed silver nanoparticles (AgNPs) in cellulose-framework through photocatalytic reaction.
34294292	2	8	theme	monodispersed	311:323	arg1	AgNPs					347:351	AgNPs	347:351	AgNPs	347:351	In this study, As an eco-friendly and facile method, we prepared monodispersed silver nanoparticles (AgNPs) in cellulose-framework through photocatalytic reaction.
34294292	2	8	theme	monodispersed	311:323	arg1	nanoparticles					332:344	monodispersed silver nanoparticles	311:344	monodispersed silver nanoparticles (AgNPs)	311:352	In this study, As an eco-friendly and facile method, we prepared monodispersed silver nanoparticles (AgNPs) in cellulose-framework through photocatalytic reaction.
34294292	7	9	theme	simvastatin	945:955	arg1	easy					1023:1026	easy	1023:1026	easy	1023:1026	In addition, it was suggested that the gradual release of simvastatin contained in the nanofibers and excellent mineralization would be easy to apply to bone regeneration.
34294292	7	9	theme	simvastatin	945:955	arg1	release					934:940	the gradual release	922:940	the gradual release of simvastatin contained in the nanofibers and excellent mineralization	922:1012	In addition, it was suggested that the gradual release of simvastatin contained in the nanofibers and excellent mineralization would be easy to apply to bone regeneration.
34294292	8	10	theme	biomedical	1143:1152	arg1	fields					1154:1159	biomedical fields	1143:1159	biomedical fields	1143:1159	Therefore, the manufactured composite electrospun fiber mat can be used not only in biomedical fields but also in various applications that need to prevent the accumulation of microorganisms.
34294292	8	11	theme	manufactured	1074:1085	arg1	mat					1115:1117	the manufactured composite electrospun fiber mat	1070:1117	the manufactured composite electrospun fiber mat	1070:1117	Therefore, the manufactured composite electrospun fiber mat can be used not only in biomedical fields but also in various applications that need to prevent the accumulation of microorganisms.
34294292	0	12	theme	silver	61:66	arg1	nanoparticles					68:80	monodispersed silver nanoparticles	47:80	monodispersed silver nanoparticles	47:80	In-situ cellulose-framework templates mediated monodispersed silver nanoparticles via facile UV-light photocatalytic activity for anti-microbial functionalization.
34294292	8	13	theme	fiber	1109:1113	arg1	mat					1115:1117	the manufactured composite electrospun fiber mat	1070:1117	the manufactured composite electrospun fiber mat	1070:1117	Therefore, the manufactured composite electrospun fiber mat can be used not only in biomedical fields but also in various applications that need to prevent the accumulation of microorganisms.
34294292	6	14	from	nanofibers	786:795	arg1	monodisperse					766:777	monodisperse	766:777	monodisperse	766:777	Through STEM and EDS, it was found that the synthesized AgNPs were monodisperse in the nanofibers, and antibacterial activity was confirmed using gram-negative and gram-positive bacteria.
34294292	6	14	from	nanofibers	786:795	arg1	AgNPs					755:759	the synthesized AgNPs	739:759	the synthesized AgNPs	739:759	Through STEM and EDS, it was found that the synthesized AgNPs were monodisperse in the nanofibers, and antibacterial activity was confirmed using gram-negative and gram-positive bacteria.
34294292	1	15	theme	natural	219:225	arg1	Cellulose					164:172	Cellulose	164:172	Cellulose	164:172	Cellulose is well known as a biocompatible material or natural reducing material.
34294292	1	15	theme	natural	219:225	arg1	material					236:243	natural reducing material	219:243	natural reducing material	219:243	Cellulose is well known as a biocompatible material or natural reducing material.
34294292	3	16	theme	fiber	440:444	arg1	scaffolds					446:454	electrospun fiber scaffolds	428:454	electrospun fiber scaffolds	428:454	and we fabricated electrospun fiber scaffolds with excellent antibacterial properties and biocompatibility.
34294292	4	17	theme	cellulose-framework	569:587	arg1	change					555:560	the electrical change	540:560	the electrical change of the cellulose-framework	540:587	UV-irradiation causes the electrical change of the cellulose-framework, thereby converting Ag ions into Ag particles.
34294292	8	18	theme	electrospun	1097:1107	arg1	mat					1115:1117	the manufactured composite electrospun fiber mat	1070:1117	the manufactured composite electrospun fiber mat	1070:1117	Therefore, the manufactured composite electrospun fiber mat can be used not only in biomedical fields but also in various applications that need to prevent the accumulation of microorganisms.
34294292	1	19	theme	reducing	227:234	arg1	Cellulose					164:172	Cellulose	164:172	Cellulose	164:172	Cellulose is well known as a biocompatible material or natural reducing material.
34294292	1	19	theme	reducing	227:234	arg1	material					236:243	natural reducing material	219:243	natural reducing material	219:243	Cellulose is well known as a biocompatible material or natural reducing material.
34294292	0	20	theme	In-situ	0:6	arg1	templates					28:36	In-situ cellulose-framework templates	0:36	In-situ cellulose-framework templates	0:36	In-situ cellulose-framework templates mediated monodispersed silver nanoparticles via facile UV-light photocatalytic activity for anti-microbial functionalization.
34294292	6	21	theme	gram-positive	863:875	arg1	bacteria					877:884	gram-negative and gram-positive bacteria	845:884	gram-negative and gram-positive bacteria	845:884	Through STEM and EDS, it was found that the synthesized AgNPs were monodisperse in the nanofibers, and antibacterial activity was confirmed using gram-negative and gram-positive bacteria.
34294292	6	22	from	monodisperse	766:777	arg1	nanofibers					786:795	the nanofibers	782:795	the nanofibers	782:795	Through STEM and EDS, it was found that the synthesized AgNPs were monodisperse in the nanofibers, and antibacterial activity was confirmed using gram-negative and gram-positive bacteria.
34294292	7	23	theme	gradual	926:932	arg1	easy					1023:1026	easy	1023:1026	easy	1023:1026	In addition, it was suggested that the gradual release of simvastatin contained in the nanofibers and excellent mineralization would be easy to apply to bone regeneration.
34294292	7	23	theme	gradual	926:932	arg1	release					934:940	the gradual release	922:940	the gradual release of simvastatin contained in the nanofibers and excellent mineralization	922:1012	In addition, it was suggested that the gradual release of simvastatin contained in the nanofibers and excellent mineralization would be easy to apply to bone regeneration.
34294292	3	24	theme	electrospun	428:438	arg1	scaffolds					446:454	electrospun fiber scaffolds	428:454	electrospun fiber scaffolds	428:454	and we fabricated electrospun fiber scaffolds with excellent antibacterial properties and biocompatibility.
34294292	3	25	theme	antibacterial	471:483	arg1	properties					485:494	excellent antibacterial properties	461:494	excellent antibacterial properties	461:494	and we fabricated electrospun fiber scaffolds with excellent antibacterial properties and biocompatibility.
34294292	2	26	theme	eco-friendly	267:278	arg1	method					291:296	an eco-friendly and facile method	264:296	an eco-friendly and facile method	264:296	In this study, As an eco-friendly and facile method, we prepared monodispersed silver nanoparticles (AgNPs) in cellulose-framework through photocatalytic reaction.
34294292	2	26	theme	eco-friendly	267:278	arg1	nanoparticles					332:344	monodispersed silver nanoparticles	311:344	monodispersed silver nanoparticles (AgNPs)	311:352	In this study, As an eco-friendly and facile method, we prepared monodispersed silver nanoparticles (AgNPs) in cellulose-framework through photocatalytic reaction.
34294292	6	27	theme	synthesized	743:753	arg1	monodisperse					766:777	monodisperse	766:777	monodisperse	766:777	Through STEM and EDS, it was found that the synthesized AgNPs were monodisperse in the nanofibers, and antibacterial activity was confirmed using gram-negative and gram-positive bacteria.
34294292	6	27	theme	synthesized	743:753	arg1	AgNPs					755:759	the synthesized AgNPs	739:759	the synthesized AgNPs	739:759	Through STEM and EDS, it was found that the synthesized AgNPs were monodisperse in the nanofibers, and antibacterial activity was confirmed using gram-negative and gram-positive bacteria.
34294292	0	28	theme	monodispersed	47:59	arg1	nanoparticles					68:80	monodispersed silver nanoparticles	47:80	monodispersed silver nanoparticles	47:80	In-situ cellulose-framework templates mediated monodispersed silver nanoparticles via facile UV-light photocatalytic activity for anti-microbial functionalization.
34294292	4	29	theme	Ag	622:623	arg1	particles					625:633	Ag particles	622:633	Ag particles	622:633	UV-irradiation causes the electrical change of the cellulose-framework, thereby converting Ag ions into Ag particles.
34294292	8	30	theme	various	1173:1179	arg1	applications					1181:1192	various applications	1173:1192	various applications that need to prevent the accumulation of microorganisms	1173:1248	Therefore, the manufactured composite electrospun fiber mat can be used not only in biomedical fields but also in various applications that need to prevent the accumulation of microorganisms.
34294292	8	31	used	used	1126:1129	arg2	mat					1115:1117	the manufactured composite electrospun fiber mat	1070:1117	the manufactured composite electrospun fiber mat	1070:1117	Therefore, the manufactured composite electrospun fiber mat can be used not only in biomedical fields but also in various applications that need to prevent the accumulation of microorganisms.
34294292	4	32	theme	electrical	544:553	arg1	change					555:560	the electrical change	540:560	the electrical change of the cellulose-framework	540:587	UV-irradiation causes the electrical change of the cellulose-framework, thereby converting Ag ions into Ag particles.
34294292	6	33	theme	gram-negative	845:857	arg1	bacteria					877:884	gram-negative and gram-positive bacteria	845:884	gram-negative and gram-positive bacteria	845:884	Through STEM and EDS, it was found that the synthesized AgNPs were monodisperse in the nanofibers, and antibacterial activity was confirmed using gram-negative and gram-positive bacteria.
34294292	2	34	theme	photocatalytic	385:398	arg1	reaction					400:407	photocatalytic reaction	385:407	photocatalytic reaction	385:407	In this study, As an eco-friendly and facile method, we prepared monodispersed silver nanoparticles (AgNPs) in cellulose-framework through photocatalytic reaction.
34294292	3	35	theme	excellent	461:469	arg1	properties					485:494	excellent antibacterial properties	461:494	excellent antibacterial properties	461:494	and we fabricated electrospun fiber scaffolds with excellent antibacterial properties and biocompatibility.
34294292	5	36	theme	three-electrode	649:663	arg1	system					665:670	a three-electrode system	647:670	a three-electrode system to confirm the phenomenon	647:696	We applied a three-electrode system to confirm the phenomenon.
34294292	7	37	theme	excellent	989:997	arg1	mineralization					999:1012	excellent mineralization	989:1012	excellent mineralization	989:1012	In addition, it was suggested that the gradual release of simvastatin contained in the nanofibers and excellent mineralization would be easy to apply to bone regeneration.
34294292	0	38	theme	UV-light	93:100	arg1	activity					117:124	facile UV-light photocatalytic activity	86:124	facile UV-light photocatalytic activity for anti-microbial functionalization	86:161	In-situ cellulose-framework templates mediated monodispersed silver nanoparticles via facile UV-light photocatalytic activity for anti-microbial functionalization.
34294292	2	39	theme	facile	284:289	arg1	method					291:296	an eco-friendly and facile method	264:296	an eco-friendly and facile method	264:296	In this study, As an eco-friendly and facile method, we prepared monodispersed silver nanoparticles (AgNPs) in cellulose-framework through photocatalytic reaction.
34294292	2	39	theme	facile	284:289	arg1	nanoparticles					332:344	monodispersed silver nanoparticles	311:344	monodispersed silver nanoparticles (AgNPs)	311:352	In this study, As an eco-friendly and facile method, we prepared monodispersed silver nanoparticles (AgNPs) in cellulose-framework through photocatalytic reaction.
34294292	0	40	theme	facile	86:91	arg1	activity					117:124	facile UV-light photocatalytic activity	86:124	facile UV-light photocatalytic activity for anti-microbial functionalization	86:161	In-situ cellulose-framework templates mediated monodispersed silver nanoparticles via facile UV-light photocatalytic activity for anti-microbial functionalization.
34294292	6	41	theme	antibacterial	802:814	arg1	activity					816:823	antibacterial activity	802:823	antibacterial activity	802:823	Through STEM and EDS, it was found that the synthesized AgNPs were monodisperse in the nanofibers, and antibacterial activity was confirmed using gram-negative and gram-positive bacteria.
34294292	0	42	theme	cellulose-framework	8:26	arg1	templates					28:36	In-situ cellulose-framework templates	0:36	In-situ cellulose-framework templates	0:36	In-situ cellulose-framework templates mediated monodispersed silver nanoparticles via facile UV-light photocatalytic activity for anti-microbial functionalization.
32366959	4	0	theme	prenatal	533:540	arg1	exposure					546:553	prenatal NNS exposure	533:553	prenatal NNS exposure	533:553	We investigated the effect of prenatal NNS exposure on postnatal adiposity and adipocyte development.
32366959	3	1	theme	early	460:464	arg1	development					466:476	early development	460:476	early development	460:476	The impact of NNS during critical periods of early development has rarely been studied.
32366959	0	2	from	cells	140:144	arg1	evidence					108:115	evidence	108:115	Nonnutritive sweetener consumption during pregnancy, adiposity, and adipocyte differentiation in offspring: evidence from humans, mice, and cells.	0:145	Nonnutritive sweetener consumption during pregnancy, adiposity, and adipocyte differentiation in offspring: evidence from humans, mice, and cells.
32366959	10	3	theme	increased	1771:1779	arg1	accumulation					1787:1798	increased lipid accumulation	1771:1798	increased lipid accumulation	1771:1798	In cultured adipocytes, sucralose exposure at early stages of differentiation caused increased lipid accumulation and expression of adipocyte differentiation genes (e.g., C/EBP-α, FABP4, and FASN).
32366959	5	4	theme	birth	626:630	arg1	N = 2298					640:647	N = 2298	640:647	N = 2298	640:647	METHODS In the CHILD birth cohort (N = 2298), we assessed maternal NNS beverage intake during pregnancy and child body composition at 3 years, controlling for maternal BMI and other potential confounders.
32366959	5	4	theme	birth	626:630	arg1	cohort					632:637	the CHILD birth cohort	616:637	the CHILD birth cohort (N = 2298)	616:648	METHODS In the CHILD birth cohort (N = 2298), we assessed maternal NNS beverage intake during pregnancy and child body composition at 3 years, controlling for maternal BMI and other potential confounders.
32366959	12	5	theme	new	2089:2091	arg1	evidence					2093:2100	new evidence that maternal NNS consumption during pregnancy may program obesity risk in offspring through effects on adiposity and adipocyte differentiation	2089:2244	new evidence that maternal NNS consumption during pregnancy may program obesity risk in offspring through effects on adiposity and adipocyte differentiation	2089:2244	CONCLUSION By triangulating evidence from humans, mice, and cultured adipocytes, this study provides new evidence that maternal NNS consumption during pregnancy may program obesity risk in offspring through effects on adiposity and adipocyte differentiation.
32366959	12	6	from	adipocytes	2057:2066	arg1	evidence					2016:2023	evidence	2016:2023	evidence from humans, mice, and cultured adipocytes	2016:2066	CONCLUSION By triangulating evidence from humans, mice, and cultured adipocytes, this study provides new evidence that maternal NNS consumption during pregnancy may program obesity risk in offspring through effects on adiposity and adipocyte differentiation.
32366959	11	7	theme	offspring	1950:1958	arg1	tissue					1929:1934	adipose tissue	1921:1934	adipose tissue of male mouse offspring born to sucralose-fed dams	1921:1985	These genes were also upregulated in adipose tissue of male mouse offspring born to sucralose-fed dams.
32366959	10	8	theme	differentiation	1748:1762	arg1	stages					1738:1743	early stages	1732:1743	early stages of differentiation	1732:1762	In cultured adipocytes, sucralose exposure at early stages of differentiation caused increased lipid accumulation and expression of adipocyte differentiation genes (e.g., C/EBP-α, FABP4, and FASN).
32366959	6	9	theme	causal	825:830	arg1	mechanisms					832:841	causal mechanisms	825:841	causal mechanisms	825:841	To investigate causal mechanisms, we fed NNS to pregnant C57BL6J mice at doses relevant to human consumption (42 mg/kg/day aspartame or 6.3 mg/kg/day sucralose), and assessed offspring until 12 weeks of age for: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance.
32366959	11	10	theme	male	1939:1942	arg1	offspring					1950:1958	male mouse offspring	1939:1958	male mouse offspring born to sucralose-fed dams	1939:1985	These genes were also upregulated in adipose tissue of male mouse offspring born to sucralose-fed dams.
32366959	6	11	theme	gene	1076:1079	arg1	expression					1081:1090	gene expression	1076:1090	gene expression	1076:1090	To investigate causal mechanisms, we fed NNS to pregnant C57BL6J mice at doses relevant to human consumption (42 mg/kg/day aspartame or 6.3 mg/kg/day sucralose), and assessed offspring until 12 weeks of age for: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance.
32366959	12	12	from	mice	2038:2041	arg1	evidence					2016:2023	evidence	2016:2023	evidence from humans, mice, and cultured adipocytes	2016:2066	CONCLUSION By triangulating evidence from humans, mice, and cultured adipocytes, this study provides new evidence that maternal NNS consumption during pregnancy may program obesity risk in offspring through effects on adiposity and adipocyte differentiation.
32366959	10	13	from	stages	1738:1743	arg1	exposure					1720:1727	sucralose exposure	1710:1727	sucralose exposure at early stages of differentiation	1710:1762	In cultured adipocytes, sucralose exposure at early stages of differentiation caused increased lipid accumulation and expression of adipocyte differentiation genes (e.g., C/EBP-α, FABP4, and FASN).
32366959	6	14	theme	human	901:905	arg1	consumption					907:917	human consumption	901:917	human consumption (42 mg/kg/day aspartame or 6.3 mg/kg/day sucralose)	901:969	To investigate causal mechanisms, we fed NNS to pregnant C57BL6J mice at doses relevant to human consumption (42 mg/kg/day aspartame or 6.3 mg/kg/day sucralose), and assessed offspring until 12 weeks of age for: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance.
32366959	7	15	from	expression	1195:1204	arg1	cells					1239:1243	cultured 3T3-L1 pre-adipocyte cells	1209:1243	cultured 3T3-L1 pre-adipocyte cells	1209:1243	We also studied the effect of sucralose on lipid accumulation and gene expression in cultured 3T3-L1 pre-adipocyte cells.
32366959	6	16	theme	morphology	1061:1070	arg1	tolerance					1113:1121	: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance	1020:1121	: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance	1020:1121	To investigate causal mechanisms, we fed NNS to pregnant C57BL6J mice at doses relevant to human consumption (42 mg/kg/day aspartame or 6.3 mg/kg/day sucralose), and assessed offspring until 12 weeks of age for: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance.
32366959	12	17	from	humans	2030:2035	arg1	evidence					2016:2023	evidence	2016:2023	evidence from humans, mice, and cultured adipocytes	2016:2066	CONCLUSION By triangulating evidence from humans, mice, and cultured adipocytes, this study provides new evidence that maternal NNS consumption during pregnancy may program obesity risk in offspring through effects on adiposity and adipocyte differentiation.
32366959	6	18	theme	relevant	889:896	arg1	doses					883:887	doses	883:887	doses relevant to human consumption (42 mg/kg/day aspartame or 6.3 mg/kg/day sucralose)	883:969	To investigate causal mechanisms, we fed NNS to pregnant C57BL6J mice at doses relevant to human consumption (42 mg/kg/day aspartame or 6.3 mg/kg/day sucralose), and assessed offspring until 12 weeks of age for: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance.
32366959	8	19	theme	mass	1355:1358	arg1	index					1360:1364	elevated body mass index	1341:1364	elevated body mass index (mean z-score difference +0.23, 95% CI 0.05-0.42 for daily vs. no consumption, adjusted for maternal BMI)	1341:1470	RESULTS In the CHILD cohort, children born to mothers who regularly consumed NNS beverages had elevated body mass index (mean z-score difference +0.23, 95% CI 0.05-0.42 for daily vs. no consumption, adjusted for maternal BMI).
32366959	10	20	theme	genes	1844:1848	arg1	expression					1804:1813	expression	1804:1813	expression of adipocyte differentiation genes (e.g., C/EBP-α, FABP4, and FASN)	1804:1881	In cultured adipocytes, sucralose exposure at early stages of differentiation caused increased lipid accumulation and expression of adipocyte differentiation genes (e.g., C/EBP-α, FABP4, and FASN).
32366959	10	20	theme	genes	1844:1848	arg1	accumulation					1787:1798	increased lipid accumulation	1771:1798	increased lipid accumulation	1771:1798	In cultured adipocytes, sucralose exposure at early stages of differentiation caused increased lipid accumulation and expression of adipocyte differentiation genes (e.g., C/EBP-α, FABP4, and FASN).
32366959	5	21	theme	maternal	663:670	arg1	intake					685:690	maternal NNS beverage intake	663:690	maternal NNS beverage intake	663:690	METHODS In the CHILD birth cohort (N = 2298), we assessed maternal NNS beverage intake during pregnancy and child body composition at 3 years, controlling for maternal BMI and other potential confounders.
32366959	5	22	theme	potential	787:795	arg1	confounders					797:807	other potential confounders	781:807	other potential confounders	781:807	METHODS In the CHILD birth cohort (N = 2298), we assessed maternal NNS beverage intake during pregnancy and child body composition at 3 years, controlling for maternal BMI and other potential confounders.
32366959	12	23	theme	obesity	2161:2167	arg1	risk					2169:2172	obesity risk	2161:2172	obesity risk	2161:2172	CONCLUSION By triangulating evidence from humans, mice, and cultured adipocytes, this study provides new evidence that maternal NNS consumption during pregnancy may program obesity risk in offspring through effects on adiposity and adipocyte differentiation.
32366959	10	24	theme	adipocyte	1818:1826	arg1	genes					1844:1848	adipocyte differentiation genes	1818:1848	adipocyte differentiation genes (e.g., C/EBP-α, FABP4, and FASN)	1818:1881	In cultured adipocytes, sucralose exposure at early stages of differentiation caused increased lipid accumulation and expression of adipocyte differentiation genes (e.g., C/EBP-α, FABP4, and FASN).
32366959	12	25	theme	NNS	2116:2118	arg1	consumption					2120:2130	maternal NNS consumption	2107:2130	maternal NNS consumption during pregnancy	2107:2147	CONCLUSION By triangulating evidence from humans, mice, and cultured adipocytes, this study provides new evidence that maternal NNS consumption during pregnancy may program obesity risk in offspring through effects on adiposity and adipocyte differentiation.
32366959	6	26	theme	C57BL6J	867:873	arg1	mice					875:878	pregnant C57BL6J mice	858:878	pregnant C57BL6J mice	858:878	To investigate causal mechanisms, we fed NNS to pregnant C57BL6J mice at doses relevant to human consumption (42 mg/kg/day aspartame or 6.3 mg/kg/day sucralose), and assessed offspring until 12 weeks of age for: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance.
32366959	8	27	theme	z-score	1372:1378	arg1	difference					1380:1389	mean z-score difference +0.23	1367:1395	mean z-score difference +0.23	1367:1395	RESULTS In the CHILD cohort, children born to mothers who regularly consumed NNS beverages had elevated body mass index (mean z-score difference +0.23, 95% CI 0.05-0.42 for daily vs. no consumption, adjusted for maternal BMI).
32366959	7	28	from	effect	1144:1149	arg1	accumulation					1173:1184	lipid accumulation	1167:1184	lipid accumulation	1167:1184	We also studied the effect of sucralose on lipid accumulation and gene expression in cultured 3T3-L1 pre-adipocyte cells.
32366959	7	28	from	effect	1144:1149	arg1	expression					1195:1204	gene expression	1190:1204	gene expression	1190:1204	We also studied the effect of sucralose on lipid accumulation and gene expression in cultured 3T3-L1 pre-adipocyte cells.
32366959	4	29	theme	exposure	546:553	arg1	effect					523:528	the effect	519:528	the effect of prenatal NNS exposure on postnatal adiposity and adipocyte development	519:602	We investigated the effect of prenatal NNS exposure on postnatal adiposity and adipocyte development.
32366959	11	30	theme	sucralose-fed	1968:1980	arg1	dams					1982:1985	sucralose-fed dams	1968:1985	sucralose-fed dams	1968:1985	These genes were also upregulated in adipose tissue of male mouse offspring born to sucralose-fed dams.
32366959	6	31	theme	6.3 mg/kg/day	946:958	arg1	sucralose					960:968	6.3 mg/kg/day sucralose	946:968	6.3 mg/kg/day sucralose	946:968	To investigate causal mechanisms, we fed NNS to pregnant C57BL6J mice at doses relevant to human consumption (42 mg/kg/day aspartame or 6.3 mg/kg/day sucralose), and assessed offspring until 12 weeks of age for: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance.
32366959	0	32	from	consumption	23:33	arg1	offspring					97:105	offspring	97:105	offspring	97:105	Nonnutritive sweetener consumption during pregnancy, adiposity, and adipocyte differentiation in offspring: evidence from humans, mice, and cells.
32366959	5	33	theme	body	719:722	arg1	composition					724:734	child body composition	713:734	child body composition	713:734	METHODS In the CHILD birth cohort (N = 2298), we assessed maternal NNS beverage intake during pregnancy and child body composition at 3 years, controlling for maternal BMI and other potential confounders.
32366959	10	34	dep	C/EBP-α	1857:1863	arg1	e.g.					1851:1854	e.g.	1851:1854	e.g.	1851:1854	In cultured adipocytes, sucralose exposure at early stages of differentiation caused increased lipid accumulation and expression of adipocyte differentiation genes (e.g., C/EBP-α, FABP4, and FASN).
32366959	1	35	theme	BACKGROUND	147:156	arg1	Obesity					158:164	BACKGROUND Obesity	147:164	BACKGROUND Obesity	147:164	BACKGROUND Obesity often originates in early life, and is linked to excess sugar intake.
32366959	9	36	theme	%	1609:1609	arg1	increase					1611:1618	15% increase	1607:1618	15% increase in body fat	1607:1630	In mice, maternal NNS caused elevated body weight, adiposity, and insulin resistance in offspring, especially in males (e.g., 47% and 15% increase in body fat for aspartame and sucralose vs. controls, p < 0.001).
32366959	8	37	theme	CHILD	1261:1265	arg1	cohort					1267:1272	the CHILD cohort	1257:1272	the CHILD cohort	1257:1272	RESULTS In the CHILD cohort, children born to mothers who regularly consumed NNS beverages had elevated body mass index (mean z-score difference +0.23, 95% CI 0.05-0.42 for daily vs. no consumption, adjusted for maternal BMI).
32366959	5	38	theme	beverage	676:683	arg1	intake					685:690	maternal NNS beverage intake	663:690	maternal NNS beverage intake	663:690	METHODS In the CHILD birth cohort (N = 2298), we assessed maternal NNS beverage intake during pregnancy and child body composition at 3 years, controlling for maternal BMI and other potential confounders.
32366959	0	39	theme	Nonnutritive	0:11	arg1	consumption					23:33	Nonnutritive sweetener consumption	0:33	Nonnutritive sweetener consumption during pregnancy, adiposity, and adipocyte differentiation in offspring: evidence from humans, mice, and cells.	0:145	Nonnutritive sweetener consumption during pregnancy, adiposity, and adipocyte differentiation in offspring: evidence from humans, mice, and cells.
32366959	2	40	theme	weight	381:386	arg1	gain					388:391	weight gain	381:391	weight gain	381:391	Nonnutritive sweeteners (NNS) are widely consumed as "healthier" alternatives to sugar, yet recent evidence suggests NNS may adversely influence weight gain and metabolic health.
32366959	5	41	theme	maternal	764:771	arg1	BMI					773:775	maternal BMI	764:775	maternal BMI	764:775	METHODS In the CHILD birth cohort (N = 2298), we assessed maternal NNS beverage intake during pregnancy and child body composition at 3 years, controlling for maternal BMI and other potential confounders.
32366959	8	42	dep	difference	1380:1389	arg1	CI					1402:1403	95% CI 0.05-0.42	1398:1413	95% CI 0.05-0.42	1398:1413	RESULTS In the CHILD cohort, children born to mothers who regularly consumed NNS beverages had elevated body mass index (mean z-score difference +0.23, 95% CI 0.05-0.42 for daily vs. no consumption, adjusted for maternal BMI).
32366959	7	43	theme	pre-adipocyte	1225:1237	arg1	cells					1239:1243	cultured 3T3-L1 pre-adipocyte cells	1209:1243	cultured 3T3-L1 pre-adipocyte cells	1209:1243	We also studied the effect of sucralose on lipid accumulation and gene expression in cultured 3T3-L1 pre-adipocyte cells.
32366959	8	44	dep	RESULTS	1246:1252	arg1	had					1337:1339	had	1337:1339	had elevated body mass index (mean z-score difference +0.23, 95% CI 0.05-0.42 for daily vs. no consumption, adjusted for maternal BMI)	1337:1470	RESULTS In the CHILD cohort, children born to mothers who regularly consumed NNS beverages had elevated body mass index (mean z-score difference +0.23, 95% CI 0.05-0.42 for daily vs. no consumption, adjusted for maternal BMI).
32366959	7	45	theme	cultured	1209:1216	arg1	cells					1239:1243	cultured 3T3-L1 pre-adipocyte cells	1209:1243	cultured 3T3-L1 pre-adipocyte cells	1209:1243	We also studied the effect of sucralose on lipid accumulation and gene expression in cultured 3T3-L1 pre-adipocyte cells.
32366959	8	46	theme	maternal	1458:1465	arg1	BMI					1467:1469	maternal BMI	1458:1469	maternal BMI	1458:1469	RESULTS In the CHILD cohort, children born to mothers who regularly consumed NNS beverages had elevated body mass index (mean z-score difference +0.23, 95% CI 0.05-0.42 for daily vs. no consumption, adjusted for maternal BMI).
32366959	8	47	theme	NNS	1323:1325	arg1	beverages					1327:1335	NNS beverages	1323:1335	NNS beverages	1323:1335	RESULTS In the CHILD cohort, children born to mothers who regularly consumed NNS beverages had elevated body mass index (mean z-score difference +0.23, 95% CI 0.05-0.42 for daily vs. no consumption, adjusted for maternal BMI).
32366959	3	48	theme	critical	440:447	arg1	periods					449:455	critical periods	440:455	critical periods of early development	440:476	The impact of NNS during critical periods of early development has rarely been studied.
32366959	8	49	dep	index	1360:1364	arg1	difference					1380:1389	mean z-score difference +0.23	1367:1395	mean z-score difference +0.23	1367:1395	RESULTS In the CHILD cohort, children born to mothers who regularly consumed NNS beverages had elevated body mass index (mean z-score difference +0.23, 95% CI 0.05-0.42 for daily vs. no consumption, adjusted for maternal BMI).
32366959	9	50	theme	maternal	1482:1489	arg1	NNS					1491:1493	maternal NNS	1482:1493	maternal NNS	1482:1493	In mice, maternal NNS caused elevated body weight, adiposity, and insulin resistance in offspring, especially in males (e.g., 47% and 15% increase in body fat for aspartame and sucralose vs. controls, p < 0.001).
32366959	3	51	theme	NNS	429:431	arg1	impact					419:424	The impact	415:424	The impact of NNS during critical periods of early development	415:476	The impact of NNS during critical periods of early development has rarely been studied.
32366959	0	52	dep	consumption	23:33	arg1	evidence					108:115	evidence	108:115	Nonnutritive sweetener consumption during pregnancy, adiposity, and adipocyte differentiation in offspring: evidence from humans, mice, and cells.	0:145	Nonnutritive sweetener consumption during pregnancy, adiposity, and adipocyte differentiation in offspring: evidence from humans, mice, and cells.
32366959	0	53	from	mice	130:133	arg1	evidence					108:115	evidence	108:115	Nonnutritive sweetener consumption during pregnancy, adiposity, and adipocyte differentiation in offspring: evidence from humans, mice, and cells.	0:145	Nonnutritive sweetener consumption during pregnancy, adiposity, and adipocyte differentiation in offspring: evidence from humans, mice, and cells.
32366959	10	54	theme	cultured	1689:1696	arg1	adipocytes					1698:1707	cultured adipocytes	1689:1707	cultured adipocytes	1689:1707	In cultured adipocytes, sucralose exposure at early stages of differentiation caused increased lipid accumulation and expression of adipocyte differentiation genes (e.g., C/EBP-α, FABP4, and FASN).
32366959	8	55	theme	body	1350:1353	arg1	index					1360:1364	elevated body mass index	1341:1364	elevated body mass index (mean z-score difference +0.23, 95% CI 0.05-0.42 for daily vs. no consumption, adjusted for maternal BMI)	1341:1470	RESULTS In the CHILD cohort, children born to mothers who regularly consumed NNS beverages had elevated body mass index (mean z-score difference +0.23, 95% CI 0.05-0.42 for daily vs. no consumption, adjusted for maternal BMI).
32366959	3	56	theme	development	466:476	arg1	periods					449:455	critical periods	440:455	critical periods of early development	440:476	The impact of NNS during critical periods of early development has rarely been studied.
32366959	6	57	theme	pregnant	858:865	arg1	mice					875:878	pregnant C57BL6J mice	858:878	pregnant C57BL6J mice	858:878	To investigate causal mechanisms, we fed NNS to pregnant C57BL6J mice at doses relevant to human consumption (42 mg/kg/day aspartame or 6.3 mg/kg/day sucralose), and assessed offspring until 12 weeks of age for: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance.
32366959	9	58	theme	body	1511:1514	arg1	weight					1516:1521	elevated body weight	1502:1521	elevated body weight	1502:1521	In mice, maternal NNS caused elevated body weight, adiposity, and insulin resistance in offspring, especially in males (e.g., 47% and 15% increase in body fat for aspartame and sucralose vs. controls, p < 0.001).
32366959	7	59	theme	lipid	1167:1171	arg1	accumulation					1173:1184	lipid accumulation	1167:1184	lipid accumulation	1167:1184	We also studied the effect of sucralose on lipid accumulation and gene expression in cultured 3T3-L1 pre-adipocyte cells.
32366959	0	60	from	humans	122:127	arg1	evidence					108:115	evidence	108:115	Nonnutritive sweetener consumption during pregnancy, adiposity, and adipocyte differentiation in offspring: evidence from humans, mice, and cells.	0:145	Nonnutritive sweetener consumption during pregnancy, adiposity, and adipocyte differentiation in offspring: evidence from humans, mice, and cells.
32366959	10	61	theme	early	1732:1736	arg1	stages					1738:1743	early stages	1732:1743	early stages of differentiation	1732:1762	In cultured adipocytes, sucralose exposure at early stages of differentiation caused increased lipid accumulation and expression of adipocyte differentiation genes (e.g., C/EBP-α, FABP4, and FASN).
32366959	1	62	theme	sugar	222:226	arg1	intake					228:233	excess sugar intake	215:233	excess sugar intake	215:233	BACKGROUND Obesity often originates in early life, and is linked to excess sugar intake.
32366959	8	63	theme	95	1398:1399	arg1	%					1400:1400	%	1400:1400	%	1400:1400	RESULTS In the CHILD cohort, children born to mothers who regularly consumed NNS beverages had elevated body mass index (mean z-score difference +0.23, 95% CI 0.05-0.42 for daily vs. no consumption, adjusted for maternal BMI).
32366959	7	64	theme	sucralose	1154:1162	arg1	effect					1144:1149	the effect	1140:1149	the effect of sucralose on lipid accumulation and gene expression in cultured 3T3-L1 pre-adipocyte cells	1140:1243	We also studied the effect of sucralose on lipid accumulation and gene expression in cultured 3T3-L1 pre-adipocyte cells.
32366959	5	65	theme	CHILD	620:624	arg1	N = 2298					640:647	N = 2298	640:647	N = 2298	640:647	METHODS In the CHILD birth cohort (N = 2298), we assessed maternal NNS beverage intake during pregnancy and child body composition at 3 years, controlling for maternal BMI and other potential confounders.
32366959	5	65	theme	CHILD	620:624	arg1	cohort					632:637	the CHILD birth cohort	616:637	the CHILD birth cohort (N = 2298)	616:648	METHODS In the CHILD birth cohort (N = 2298), we assessed maternal NNS beverage intake during pregnancy and child body composition at 3 years, controlling for maternal BMI and other potential confounders.
32366959	11	66	theme	adipose	1921:1927	arg1	tissue					1929:1934	adipose tissue	1921:1934	adipose tissue of male mouse offspring born to sucralose-fed dams	1921:1985	These genes were also upregulated in adipose tissue of male mouse offspring born to sucralose-fed dams.
32366959	6	67	theme	glucose	1093:1099	arg1	tolerance					1113:1121	: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance	1020:1121	: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance	1020:1121	To investigate causal mechanisms, we fed NNS to pregnant C57BL6J mice at doses relevant to human consumption (42 mg/kg/day aspartame or 6.3 mg/kg/day sucralose), and assessed offspring until 12 weeks of age for: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance.
32366959	4	68	theme	NNS	542:544	arg1	exposure					546:553	prenatal NNS exposure	533:553	prenatal NNS exposure	533:553	We investigated the effect of prenatal NNS exposure on postnatal adiposity and adipocyte development.
32366959	7	69	theme	gene	1190:1193	arg1	expression					1195:1204	gene expression	1190:1204	gene expression	1190:1204	We also studied the effect of sucralose on lipid accumulation and gene expression in cultured 3T3-L1 pre-adipocyte cells.
32366959	12	70	from	effects	2195:2201	arg1	adiposity					2206:2214	adiposity	2206:2214	adiposity	2206:2214	CONCLUSION By triangulating evidence from humans, mice, and cultured adipocytes, this study provides new evidence that maternal NNS consumption during pregnancy may program obesity risk in offspring through effects on adiposity and adipocyte differentiation.
32366959	12	70	from	effects	2195:2201	arg1	differentiation					2230:2244	adipocyte differentiation	2220:2244	adipocyte differentiation	2220:2244	CONCLUSION By triangulating evidence from humans, mice, and cultured adipocytes, this study provides new evidence that maternal NNS consumption during pregnancy may program obesity risk in offspring through effects on adiposity and adipocyte differentiation.
32366959	9	71	theme	insulin	1539:1545	arg1	resistance					1547:1556	insulin resistance	1539:1556	insulin resistance	1539:1556	In mice, maternal NNS caused elevated body weight, adiposity, and insulin resistance in offspring, especially in males (e.g., 47% and 15% increase in body fat for aspartame and sucralose vs. controls, p < 0.001).
32366959	7	72	from	accumulation	1173:1184	arg1	cells					1239:1243	cultured 3T3-L1 pre-adipocyte cells	1209:1243	cultured 3T3-L1 pre-adipocyte cells	1209:1243	We also studied the effect of sucralose on lipid accumulation and gene expression in cultured 3T3-L1 pre-adipocyte cells.
32366959	11	73	theme	mouse	1944:1948	arg1	offspring					1950:1958	male mouse offspring	1939:1958	male mouse offspring born to sucralose-fed dams	1939:1985	These genes were also upregulated in adipose tissue of male mouse offspring born to sucralose-fed dams.
32366959	1	74	theme	early	186:190	arg1	life					192:195	early life	186:195	early life	186:195	BACKGROUND Obesity often originates in early life, and is linked to excess sugar intake.
32366959	6	75	theme	tissue	1054:1059	arg1	morphology					1061:1070	adipose tissue morphology	1046:1070	adipose tissue morphology	1046:1070	To investigate causal mechanisms, we fed NNS to pregnant C57BL6J mice at doses relevant to human consumption (42 mg/kg/day aspartame or 6.3 mg/kg/day sucralose), and assessed offspring until 12 weeks of age for: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance.
32366959	4	76	theme	adipocyte	582:590	arg1	development					592:602	adipocyte development	582:602	adipocyte development	582:602	We investigated the effect of prenatal NNS exposure on postnatal adiposity and adipocyte development.
32366959	10	77	theme	lipid	1781:1785	arg1	accumulation					1787:1798	increased lipid accumulation	1771:1798	increased lipid accumulation	1771:1798	In cultured adipocytes, sucralose exposure at early stages of differentiation caused increased lipid accumulation and expression of adipocyte differentiation genes (e.g., C/EBP-α, FABP4, and FASN).
32366959	1	78	attach	linked	205:210	arg2	Obesity					158:164	BACKGROUND Obesity	147:164	BACKGROUND Obesity	147:164	BACKGROUND Obesity often originates in early life, and is linked to excess sugar intake.
32366959	1	78	attach	linked	205:210	arg1	intake					228:233	excess sugar intake	215:233	excess sugar intake	215:233	BACKGROUND Obesity often originates in early life, and is linked to excess sugar intake.
32366959	5	79	theme	other	781:785	arg1	confounders					797:807	other potential confounders	781:807	other potential confounders	781:807	METHODS In the CHILD birth cohort (N = 2298), we assessed maternal NNS beverage intake during pregnancy and child body composition at 3 years, controlling for maternal BMI and other potential confounders.
32366959	8	80	theme	mean	1367:1370	arg1	difference					1380:1389	mean z-score difference +0.23	1367:1395	mean z-score difference +0.23	1367:1395	RESULTS In the CHILD cohort, children born to mothers who regularly consumed NNS beverages had elevated body mass index (mean z-score difference +0.23, 95% CI 0.05-0.42 for daily vs. no consumption, adjusted for maternal BMI).
32366959	10	81	theme	differentiation	1828:1842	arg1	genes					1844:1848	adipocyte differentiation genes	1818:1848	adipocyte differentiation genes (e.g., C/EBP-α, FABP4, and FASN)	1818:1881	In cultured adipocytes, sucralose exposure at early stages of differentiation caused increased lipid accumulation and expression of adipocyte differentiation genes (e.g., C/EBP-α, FABP4, and FASN).
32366959	2	82	theme	Nonnutritive	236:247	arg1	NNS					261:263	NNS	261:263	NNS	261:263	Nonnutritive sweeteners (NNS) are widely consumed as "healthier" alternatives to sugar, yet recent evidence suggests NNS may adversely influence weight gain and metabolic health.
32366959	2	82	theme	Nonnutritive	236:247	arg1	sweeteners					249:258	Nonnutritive sweeteners	236:258	Nonnutritive sweeteners (NNS)	236:264	Nonnutritive sweeteners (NNS) are widely consumed as "healthier" alternatives to sugar, yet recent evidence suggests NNS may adversely influence weight gain and metabolic health.
32366959	2	82	theme	Nonnutritive	236:247	arg1	alternatives					301:312	"healthier" alternatives	289:312	"healthier" alternatives to sugar	289:321	Nonnutritive sweeteners (NNS) are widely consumed as "healthier" alternatives to sugar, yet recent evidence suggests NNS may adversely influence weight gain and metabolic health.
32366959	6	83	dep	consumption	907:917	arg1	aspartame					933:941	42 mg/kg/day aspartame	920:941	42 mg/kg/day aspartame	920:941	To investigate causal mechanisms, we fed NNS to pregnant C57BL6J mice at doses relevant to human consumption (42 mg/kg/day aspartame or 6.3 mg/kg/day sucralose), and assessed offspring until 12 weeks of age for: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance.
32366959	6	83	dep	consumption	907:917	arg1	sucralose					960:968	6.3 mg/kg/day sucralose	946:968	6.3 mg/kg/day sucralose	946:968	To investigate causal mechanisms, we fed NNS to pregnant C57BL6J mice at doses relevant to human consumption (42 mg/kg/day aspartame or 6.3 mg/kg/day sucralose), and assessed offspring until 12 weeks of age for: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance.
32366959	0	84	theme	adipocyte	68:76	arg1	differentiation					78:92	adipocyte differentiation	68:92	adipocyte differentiation	68:92	Nonnutritive sweetener consumption during pregnancy, adiposity, and adipocyte differentiation in offspring: evidence from humans, mice, and cells.
32366959	8	85	theme	%	1400:1400	arg1	CI					1402:1403	95% CI 0.05-0.42	1398:1413	95% CI 0.05-0.42	1398:1413	RESULTS In the CHILD cohort, children born to mothers who regularly consumed NNS beverages had elevated body mass index (mean z-score difference +0.23, 95% CI 0.05-0.42 for daily vs. no consumption, adjusted for maternal BMI).
32366959	6	86	theme	insulin	1105:1111	arg1	tolerance					1113:1121	: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance	1020:1121	: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance	1020:1121	To investigate causal mechanisms, we fed NNS to pregnant C57BL6J mice at doses relevant to human consumption (42 mg/kg/day aspartame or 6.3 mg/kg/day sucralose), and assessed offspring until 12 weeks of age for: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance.
32366959	5	87	theme	child	713:717	arg1	composition					724:734	child body composition	713:734	child body composition	713:734	METHODS In the CHILD birth cohort (N = 2298), we assessed maternal NNS beverage intake during pregnancy and child body composition at 3 years, controlling for maternal BMI and other potential confounders.
32366959	9	88	theme	15	1607:1608	arg1	%					1609:1609	%	1609:1609	%	1609:1609	In mice, maternal NNS caused elevated body weight, adiposity, and insulin resistance in offspring, especially in males (e.g., 47% and 15% increase in body fat for aspartame and sucralose vs. controls, p < 0.001).
32366959	12	89	theme	maternal	2107:2114	arg1	consumption					2120:2130	maternal NNS consumption	2107:2130	maternal NNS consumption during pregnancy	2107:2147	CONCLUSION By triangulating evidence from humans, mice, and cultured adipocytes, this study provides new evidence that maternal NNS consumption during pregnancy may program obesity risk in offspring through effects on adiposity and adipocyte differentiation.
32366959	5	90	theme	NNS	672:674	arg1	intake					685:690	maternal NNS beverage intake	663:690	maternal NNS beverage intake	663:690	METHODS In the CHILD birth cohort (N = 2298), we assessed maternal NNS beverage intake during pregnancy and child body composition at 3 years, controlling for maternal BMI and other potential confounders.
32366959	6	91	theme	42 mg/kg/day	920:931	arg1	aspartame					933:941	42 mg/kg/day aspartame	920:941	42 mg/kg/day aspartame	920:941	To investigate causal mechanisms, we fed NNS to pregnant C57BL6J mice at doses relevant to human consumption (42 mg/kg/day aspartame or 6.3 mg/kg/day sucralose), and assessed offspring until 12 weeks of age for: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance.
32366959	6	92	theme	age	1013:1015	arg1	weeks					1004:1008	12 weeks	1001:1008	12 weeks of age for: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance	1001:1121	To investigate causal mechanisms, we fed NNS to pregnant C57BL6J mice at doses relevant to human consumption (42 mg/kg/day aspartame or 6.3 mg/kg/day sucralose), and assessed offspring until 12 weeks of age for: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance.
32366959	9	93	theme	body	1623:1626	arg1	fat					1628:1630	body fat	1623:1630	body fat	1623:1630	In mice, maternal NNS caused elevated body weight, adiposity, and insulin resistance in offspring, especially in males (e.g., 47% and 15% increase in body fat for aspartame and sucralose vs. controls, p < 0.001).
32366959	12	94	theme	cultured	2048:2055	arg1	adipocytes					2057:2066	cultured adipocytes	2048:2066	cultured adipocytes	2048:2066	CONCLUSION By triangulating evidence from humans, mice, and cultured adipocytes, this study provides new evidence that maternal NNS consumption during pregnancy may program obesity risk in offspring through effects on adiposity and adipocyte differentiation.
32366959	0	95	theme	sweetener	13:21	arg1	consumption					23:33	Nonnutritive sweetener consumption	0:33	Nonnutritive sweetener consumption during pregnancy, adiposity, and adipocyte differentiation in offspring: evidence from humans, mice, and cells.	0:145	Nonnutritive sweetener consumption during pregnancy, adiposity, and adipocyte differentiation in offspring: evidence from humans, mice, and cells.
32366959	2	96	theme	metabolic	397:405	arg1	health					407:412	metabolic health	397:412	metabolic health	397:412	Nonnutritive sweeteners (NNS) are widely consumed as "healthier" alternatives to sugar, yet recent evidence suggests NNS may adversely influence weight gain and metabolic health.
32366959	2	97	theme	recent	328:333	arg1	evidence					335:342	recent evidence	328:342	recent evidence	328:342	Nonnutritive sweeteners (NNS) are widely consumed as "healthier" alternatives to sugar, yet recent evidence suggests NNS may adversely influence weight gain and metabolic health.
32366959	7	98	theme	3T3-L1	1218:1223	arg1	cells					1239:1243	cultured 3T3-L1 pre-adipocyte cells	1209:1243	cultured 3T3-L1 pre-adipocyte cells	1209:1243	We also studied the effect of sucralose on lipid accumulation and gene expression in cultured 3T3-L1 pre-adipocyte cells.
32366959	8	99	contain	had	1337:1339	arg2	index					1360:1364	elevated body mass index	1341:1364	elevated body mass index (mean z-score difference +0.23, 95% CI 0.05-0.42 for daily vs. no consumption, adjusted for maternal BMI)	1341:1470	RESULTS In the CHILD cohort, children born to mothers who regularly consumed NNS beverages had elevated body mass index (mean z-score difference +0.23, 95% CI 0.05-0.42 for daily vs. no consumption, adjusted for maternal BMI).
32366959	8	99	contain	had	1337:1339	arg1	children					1275:1282	children	1275:1282	children born to mothers who regularly consumed NNS beverages	1275:1335	RESULTS In the CHILD cohort, children born to mothers who regularly consumed NNS beverages had elevated body mass index (mean z-score difference +0.23, 95% CI 0.05-0.42 for daily vs. no consumption, adjusted for maternal BMI).
32366959	5	100	dep	METHODS	605:611	arg1	assessed					654:661	assessed	654:661	assessed	654:661	METHODS In the CHILD birth cohort (N = 2298), we assessed maternal NNS beverage intake during pregnancy and child body composition at 3 years, controlling for maternal BMI and other potential confounders.
32366959	9	101	from	increase	1611:1618	arg1	fat					1628:1630	body fat	1623:1630	body fat	1623:1630	In mice, maternal NNS caused elevated body weight, adiposity, and insulin resistance in offspring, especially in males (e.g., 47% and 15% increase in body fat for aspartame and sucralose vs. controls, p < 0.001).
32366959	6	102	theme	adipose	1046:1052	arg1	morphology					1061:1070	adipose tissue morphology	1046:1070	adipose tissue morphology	1046:1070	To investigate causal mechanisms, we fed NNS to pregnant C57BL6J mice at doses relevant to human consumption (42 mg/kg/day aspartame or 6.3 mg/kg/day sucralose), and assessed offspring until 12 weeks of age for: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance.
32366959	9	103	dep	%	1601:1601	arg1	e.g.					1593:1596	e.g.	1593:1596	e.g.	1593:1596	In mice, maternal NNS caused elevated body weight, adiposity, and insulin resistance in offspring, especially in males (e.g., 47% and 15% increase in body fat for aspartame and sucralose vs. controls, p < 0.001).
32366959	9	104	from	controls	1664:1671	arg1	fat					1628:1630	body fat	1623:1630	body fat	1623:1630	In mice, maternal NNS caused elevated body weight, adiposity, and insulin resistance in offspring, especially in males (e.g., 47% and 15% increase in body fat for aspartame and sucralose vs. controls, p < 0.001).
32366959	10	105	theme	sucralose	1710:1718	arg1	exposure					1720:1727	sucralose exposure	1710:1727	sucralose exposure at early stages of differentiation	1710:1762	In cultured adipocytes, sucralose exposure at early stages of differentiation caused increased lipid accumulation and expression of adipocyte differentiation genes (e.g., C/EBP-α, FABP4, and FASN).
32366959	2	106	theme	"	299:299	arg1	sweeteners					249:258	Nonnutritive sweeteners	236:258	Nonnutritive sweeteners (NNS)	236:264	Nonnutritive sweeteners (NNS) are widely consumed as "healthier" alternatives to sugar, yet recent evidence suggests NNS may adversely influence weight gain and metabolic health.
32366959	2	106	theme	"	299:299	arg1	alternatives					301:312	"healthier" alternatives	289:312	"healthier" alternatives to sugar	289:321	Nonnutritive sweeteners (NNS) are widely consumed as "healthier" alternatives to sugar, yet recent evidence suggests NNS may adversely influence weight gain and metabolic health.
32366959	6	107	theme	adiposity	1035:1043	arg1	tolerance					1113:1121	: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance	1020:1121	: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance	1020:1121	To investigate causal mechanisms, we fed NNS to pregnant C57BL6J mice at doses relevant to human consumption (42 mg/kg/day aspartame or 6.3 mg/kg/day sucralose), and assessed offspring until 12 weeks of age for: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance.
32366959	4	108	theme	postnatal	558:566	arg1	adiposity					568:576	postnatal adiposity	558:576	postnatal adiposity	558:576	We investigated the effect of prenatal NNS exposure on postnatal adiposity and adipocyte development.
32366959	6	109	theme	body	1022:1025	arg1	weight					1027:1032	body weight	1022:1032	body weight	1022:1032	To investigate causal mechanisms, we fed NNS to pregnant C57BL6J mice at doses relevant to human consumption (42 mg/kg/day aspartame or 6.3 mg/kg/day sucralose), and assessed offspring until 12 weeks of age for: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance.
32366959	9	110	from	%	1601:1601	arg1	fat					1628:1630	body fat	1623:1630	body fat	1623:1630	In mice, maternal NNS caused elevated body weight, adiposity, and insulin resistance in offspring, especially in males (e.g., 47% and 15% increase in body fat for aspartame and sucralose vs. controls, p < 0.001).
32366959	9	111	theme	elevated	1502:1509	arg1	weight					1516:1521	elevated body weight	1502:1521	elevated body weight	1502:1521	In mice, maternal NNS caused elevated body weight, adiposity, and insulin resistance in offspring, especially in males (e.g., 47% and 15% increase in body fat for aspartame and sucralose vs. controls, p < 0.001).
32366959	6	112	theme	weight	1027:1032	arg1	tolerance					1113:1121	: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance	1020:1121	: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance	1020:1121	To investigate causal mechanisms, we fed NNS to pregnant C57BL6J mice at doses relevant to human consumption (42 mg/kg/day aspartame or 6.3 mg/kg/day sucralose), and assessed offspring until 12 weeks of age for: body weight, adiposity, adipose tissue morphology and gene expression, glucose and insulin tolerance.
32366959	12	113	theme	adipocyte	2220:2228	arg1	differentiation					2230:2244	adipocyte differentiation	2220:2244	adipocyte differentiation	2220:2244	CONCLUSION By triangulating evidence from humans, mice, and cultured adipocytes, this study provides new evidence that maternal NNS consumption during pregnancy may program obesity risk in offspring through effects on adiposity and adipocyte differentiation.
32366959	8	114	theme	elevated	1341:1348	arg1	index					1360:1364	elevated body mass index	1341:1364	elevated body mass index (mean z-score difference +0.23, 95% CI 0.05-0.42 for daily vs. no consumption, adjusted for maternal BMI)	1341:1470	RESULTS In the CHILD cohort, children born to mothers who regularly consumed NNS beverages had elevated body mass index (mean z-score difference +0.23, 95% CI 0.05-0.42 for daily vs. no consumption, adjusted for maternal BMI).
32366959	4	115	from	effect	523:528	arg1	adiposity					568:576	postnatal adiposity	558:576	postnatal adiposity	558:576	We investigated the effect of prenatal NNS exposure on postnatal adiposity and adipocyte development.
32366959	4	115	from	effect	523:528	arg1	development					592:602	adipocyte development	582:602	adipocyte development	582:602	We investigated the effect of prenatal NNS exposure on postnatal adiposity and adipocyte development.
32366959	1	116	theme	excess	215:220	arg1	intake					228:233	excess sugar intake	215:233	excess sugar intake	215:233	BACKGROUND Obesity often originates in early life, and is linked to excess sugar intake.
33140141	12	0	theme	phenotypic	1410:1419	arg1	characteristics					1421:1435	the phenotypic characteristics	1406:1435	the phenotypic characteristics	1406:1435	Based on the phenotypic characteristics, chemotaxonomic data and genotypic analyses, strain LAM7117T should be classified as a novel species of genus Arthrobacter, for which the name Arthrobacter sulfonylureivorans sp.
33140141	12	0	theme	phenotypic	1410:1419	arg1	data					1453:1456	chemotaxonomic data	1438:1456	chemotaxonomic data	1438:1456	Based on the phenotypic characteristics, chemotaxonomic data and genotypic analyses, strain LAM7117T should be classified as a novel species of genus Arthrobacter, for which the name Arthrobacter sulfonylureivorans sp.
33140141	12	0	theme	phenotypic	1410:1419	arg1	analyses					1472:1479	genotypic analyses	1462:1479	genotypic analyses	1462:1479	Based on the phenotypic characteristics, chemotaxonomic data and genotypic analyses, strain LAM7117T should be classified as a novel species of genus Arthrobacter, for which the name Arthrobacter sulfonylureivorans sp.
33140141	8	1	theme	major	971:975	arg1	anteiso-C15:0					1003:1015	anteiso-C15:0	1003:1015	anteiso-C15:0	1003:1015	The major cellular fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0.
33140141	8	1	theme	major	971:975	arg1	acids					992:996	The major cellular fatty acids	967:996	The major cellular fatty acids	967:996	The major cellular fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0.
33140141	4	2	theme	NaCl	469:472	arg1	presence					457:464	the presence	453:464	the presence of NaCl	453:472	Strain LAM7117T could grow in the presence of NaCl with concentration up to 9% (w/v).
33140141	10	3	theme	strain	1182:1187	arg1	LAM7117T					1189:1196	strain LAM7117T	1182:1196	strain LAM7117T	1182:1196	The major polar lipids present in strain LAM7117T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl inositol, two unidentified glycolipids and one unidentified lipid.
33140141	10	4	theme	major	1152:1156	arg1	lipids					1164:1169	The major polar lipids	1148:1169	The major polar lipids present in strain LAM7117T	1148:1196	The major polar lipids present in strain LAM7117T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl inositol, two unidentified glycolipids and one unidentified lipid.
33140141	10	4	theme	major	1152:1156	arg1	diphosphatidylglycerol					1203:1224	diphosphatidylglycerol	1203:1224	diphosphatidylglycerol	1203:1224	The major polar lipids present in strain LAM7117T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl inositol, two unidentified glycolipids and one unidentified lipid.
33140141	3	5	theme	optimal	328:334	arg1	temperature					336:346	The optimal temperature	324:346	The optimal temperature	324:346	The optimal temperature and pH for the growth of strain LAM7117T were 35 °C and 7.5, respectively.
33140141	3	5	theme	optimal	328:334	arg1	35 °C					394:398	35 °C	394:398	35 °C	394:398	The optimal temperature and pH for the growth of strain LAM7117T were 35 °C and 7.5, respectively.
33140141	6	6	theme	JCM	877:879	arg1	2522T					881:885	JCM 2522T	877:885	JCM 2522T	877:885	The values of digital DNA-DNA relatedness and Avery Nucleotide Identity based on the genome sequences between LAM7117T and A. crystallopoietes JCM 2522T were 21.4 and 77.4%, respectively.
33140141	8	7	theme	cellular	977:984	arg1	anteiso-C15:0					1003:1015	anteiso-C15:0	1003:1015	anteiso-C15:0	1003:1015	The major cellular fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0.
33140141	8	7	theme	cellular	977:984	arg1	acids					992:996	The major cellular fatty acids	967:996	The major cellular fatty acids	967:996	The major cellular fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0.
33140141	2	8	attach	isolated	230:237	arg2	bacterium					187:195	A gram-stain positive, aerobic, motile, rod-shaped bacterium	136:195	A gram-stain positive, aerobic, motile, rod-shaped bacterium	136:195	A gram-stain positive, aerobic, motile, rod-shaped bacterium, designated strain LAM7117T, was isolated from a sulfonylurea herbicides degrading consortium enriched with birch forest soil.
33140141	2	8	attach	isolated	230:237	arg1	herbicides					259:268	a sulfonylurea herbicides	244:268	a sulfonylurea herbicides degrading consortium enriched with birch forest soil	244:321	A gram-stain positive, aerobic, motile, rod-shaped bacterium, designated strain LAM7117T, was isolated from a sulfonylurea herbicides degrading consortium enriched with birch forest soil.
33140141	5	9	theme	A.	695:696	arg1	2522T					719:723	A. crystallopoietes JCM 2522T	695:723	A. crystallopoietes JCM 2522T (97.7%)	695:731	Strain LAM7117T formed a distinct phylogenetic subclade within the genus Arthrobacter in the phylogenetic trees built with 16S rRNA gene sequences and shared the highest similarity with A. crystallopoietes JCM 2522T (97.7%).
33140141	5	9	theme	A.	695:696	arg1	%					730:730	97.7%	726:730	97.7%	726:730	Strain LAM7117T formed a distinct phylogenetic subclade within the genus Arthrobacter in the phylogenetic trees built with 16S rRNA gene sequences and shared the highest similarity with A. crystallopoietes JCM 2522T (97.7%).
33140141	2	10	theme	sulfonylurea	246:257	arg1	herbicides					259:268	a sulfonylurea herbicides	244:268	a sulfonylurea herbicides degrading consortium enriched with birch forest soil	244:321	A gram-stain positive, aerobic, motile, rod-shaped bacterium, designated strain LAM7117T, was isolated from a sulfonylurea herbicides degrading consortium enriched with birch forest soil.
33140141	5	11	theme	16S	632:634	arg1	sequences					646:654	16S rRNA gene sequences	632:654	16S rRNA gene sequences	632:654	Strain LAM7117T formed a distinct phylogenetic subclade within the genus Arthrobacter in the phylogenetic trees built with 16S rRNA gene sequences and shared the highest similarity with A. crystallopoietes JCM 2522T (97.7%).
33140141	6	12	theme	relatedness	764:774	arg1	values					738:743	The values	734:743	The values of digital DNA-DNA relatedness and Avery Nucleotide Identity based on the genome sequences between LAM7117T and A. crystallopoietes JCM 2522T	734:885	The values of digital DNA-DNA relatedness and Avery Nucleotide Identity based on the genome sequences between LAM7117T and A. crystallopoietes JCM 2522T were 21.4 and 77.4%, respectively.
33140141	6	12	theme	relatedness	764:774	arg1	%					905:905	21.4 and 77.4%	892:905	21.4 and 77.4%	892:905	The values of digital DNA-DNA relatedness and Avery Nucleotide Identity based on the genome sequences between LAM7117T and A. crystallopoietes JCM 2522T were 21.4 and 77.4%, respectively.
33140141	2	13	theme	birch	305:309	arg1	soil					318:321	birch forest soil	305:321	birch forest soil	305:321	A gram-stain positive, aerobic, motile, rod-shaped bacterium, designated strain LAM7117T, was isolated from a sulfonylurea herbicides degrading consortium enriched with birch forest soil.
33140141	5	14	theme	rRNA	636:639	arg1	sequences					646:654	16S rRNA gene sequences	632:654	16S rRNA gene sequences	632:654	Strain LAM7117T formed a distinct phylogenetic subclade within the genus Arthrobacter in the phylogenetic trees built with 16S rRNA gene sequences and shared the highest similarity with A. crystallopoietes JCM 2522T (97.7%).
33140141	5	15	theme	genus	576:580	arg1	Arthrobacter					582:593	the genus Arthrobacter	572:593	the genus Arthrobacter	572:593	Strain LAM7117T formed a distinct phylogenetic subclade within the genus Arthrobacter in the phylogenetic trees built with 16S rRNA gene sequences and shared the highest similarity with A. crystallopoietes JCM 2522T (97.7%).
33140141	1	16	theme	sulfonylurea	58:69	arg1	consortium					92:101	a sulfonylurea herbicides degrading consortium	56:101	a sulfonylurea herbicides degrading consortium enriched with birch forest soil	56:133	nov., isolated from a sulfonylurea herbicides degrading consortium enriched with birch forest soil.
33140141	10	17	theme	phosphatidyl	1249:1260	arg1	inositol					1262:1269	phosphatidyl inositol	1249:1269	phosphatidyl inositol	1249:1269	The major polar lipids present in strain LAM7117T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl inositol, two unidentified glycolipids and one unidentified lipid.
33140141	5	18	theme	JCM	715:717	arg1	2522T					719:723	A. crystallopoietes JCM 2522T	695:723	A. crystallopoietes JCM 2522T (97.7%)	695:731	Strain LAM7117T formed a distinct phylogenetic subclade within the genus Arthrobacter in the phylogenetic trees built with 16S rRNA gene sequences and shared the highest similarity with A. crystallopoietes JCM 2522T (97.7%).
33140141	5	18	theme	JCM	715:717	arg1	%					730:730	97.7%	726:730	97.7%	726:730	Strain LAM7117T formed a distinct phylogenetic subclade within the genus Arthrobacter in the phylogenetic trees built with 16S rRNA gene sequences and shared the highest similarity with A. crystallopoietes JCM 2522T (97.7%).
33140141	6	19	theme	digital	748:754	arg1	relatedness					764:774	digital DNA-DNA relatedness	748:774	digital DNA-DNA relatedness	748:774	The values of digital DNA-DNA relatedness and Avery Nucleotide Identity based on the genome sequences between LAM7117T and A. crystallopoietes JCM 2522T were 21.4 and 77.4%, respectively.
33140141	1	20	theme	herbicides	71:80	arg1	consortium					92:101	a sulfonylurea herbicides degrading consortium	56:101	a sulfonylurea herbicides degrading consortium enriched with birch forest soil	56:133	nov., isolated from a sulfonylurea herbicides degrading consortium enriched with birch forest soil.
33140141	2	21	theme	positive	149:156	arg1	bacterium					187:195	A gram-stain positive, aerobic, motile, rod-shaped bacterium	136:195	A gram-stain positive, aerobic, motile, rod-shaped bacterium	136:195	A gram-stain positive, aerobic, motile, rod-shaped bacterium, designated strain LAM7117T, was isolated from a sulfonylurea herbicides degrading consortium enriched with birch forest soil.
33140141	1	22	theme	degrading	82:90	arg1	consortium					92:101	a sulfonylurea herbicides degrading consortium	56:101	a sulfonylurea herbicides degrading consortium enriched with birch forest soil	56:133	nov., isolated from a sulfonylurea herbicides degrading consortium enriched with birch forest soil.
33140141	3	23	theme	LAM7117T	380:387	arg1	growth					363:368	the growth	359:368	the growth of strain LAM7117T	359:387	The optimal temperature and pH for the growth of strain LAM7117T were 35 °C and 7.5, respectively.
33140141	2	24	theme	gram-stain	138:147	arg1	bacterium					187:195	A gram-stain positive, aerobic, motile, rod-shaped bacterium	136:195	A gram-stain positive, aerobic, motile, rod-shaped bacterium	136:195	A gram-stain positive, aerobic, motile, rod-shaped bacterium, designated strain LAM7117T, was isolated from a sulfonylurea herbicides degrading consortium enriched with birch forest soil.
33140141	5	25	theme	phylogenetic	602:613	arg1	trees					615:619	the phylogenetic trees	598:619	the phylogenetic trees built with 16S rRNA gene sequences	598:654	Strain LAM7117T formed a distinct phylogenetic subclade within the genus Arthrobacter in the phylogenetic trees built with 16S rRNA gene sequences and shared the highest similarity with A. crystallopoietes JCM 2522T (97.7%).
33140141	5	26	theme	distinct	534:541	arg1	subclade					556:563	a distinct phylogenetic subclade	532:563	a distinct phylogenetic subclade within the genus Arthrobacter	532:593	Strain LAM7117T formed a distinct phylogenetic subclade within the genus Arthrobacter in the phylogenetic trees built with 16S rRNA gene sequences and shared the highest similarity with A. crystallopoietes JCM 2522T (97.7%).
33140141	10	27	from	LAM7117T	1189:1196	arg1	present					1171:1177	present	1171:1177	present	1171:1177	The major polar lipids present in strain LAM7117T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl inositol, two unidentified glycolipids and one unidentified lipid.
33140141	0	28	theme	Arthrobacter	0:11	arg1	sulfonylureivorans					13:30	Arthrobacter sulfonylureivorans	0:30	Arthrobacter sulfonylureivorans	0:30	Arthrobacter sulfonylureivorans sp.
33140141	2	29	dep	positive	149:156	arg1	rod-shaped					176:185	rod-shaped	176:185	rod-shaped	176:185	A gram-stain positive, aerobic, motile, rod-shaped bacterium, designated strain LAM7117T, was isolated from a sulfonylurea herbicides degrading consortium enriched with birch forest soil.
33140141	2	29	dep	positive	149:156	arg1	motile					168:173	motile	168:173	motile	168:173	A gram-stain positive, aerobic, motile, rod-shaped bacterium, designated strain LAM7117T, was isolated from a sulfonylurea herbicides degrading consortium enriched with birch forest soil.
33140141	2	29	dep	positive	149:156	arg1	aerobic					159:165	aerobic	159:165	aerobic	159:165	A gram-stain positive, aerobic, motile, rod-shaped bacterium, designated strain LAM7117T, was isolated from a sulfonylurea herbicides degrading consortium enriched with birch forest soil.
33140141	6	30	theme	Avery	780:784	arg1	Identity					797:804	Avery Nucleotide Identity	780:804	Avery Nucleotide Identity	780:804	The values of digital DNA-DNA relatedness and Avery Nucleotide Identity based on the genome sequences between LAM7117T and A. crystallopoietes JCM 2522T were 21.4 and 77.4%, respectively.
33140141	5	31	theme	phylogenetic	543:554	arg1	subclade					556:563	a distinct phylogenetic subclade	532:563	a distinct phylogenetic subclade within the genus Arthrobacter	532:593	Strain LAM7117T formed a distinct phylogenetic subclade within the genus Arthrobacter in the phylogenetic trees built with 16S rRNA gene sequences and shared the highest similarity with A. crystallopoietes JCM 2522T (97.7%).
33140141	10	32	attach	present	1171:1177	arg2	diphosphatidylglycerol					1203:1224	diphosphatidylglycerol	1203:1224	diphosphatidylglycerol	1203:1224	The major polar lipids present in strain LAM7117T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl inositol, two unidentified glycolipids and one unidentified lipid.
33140141	10	32	attach	present	1171:1177	arg1	LAM7117T					1189:1196	strain LAM7117T	1182:1196	strain LAM7117T	1182:1196	The major polar lipids present in strain LAM7117T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl inositol, two unidentified glycolipids and one unidentified lipid.
33140141	10	32	attach	present	1171:1177	arg2	lipids					1164:1169	The major polar lipids	1148:1169	The major polar lipids present in strain LAM7117T	1148:1196	The major polar lipids present in strain LAM7117T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl inositol, two unidentified glycolipids and one unidentified lipid.
33140141	10	33	theme	unidentified	1276:1287	arg1	glycolipids					1289:1299	two unidentified glycolipids	1272:1299	two unidentified glycolipids	1272:1299	The major polar lipids present in strain LAM7117T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl inositol, two unidentified glycolipids and one unidentified lipid.
33140141	12	34	theme	Arthrobacter	1580:1591	arg1	sp					1612:1613	the name Arthrobacter sulfonylureivorans sp	1571:1613	the name Arthrobacter sulfonylureivorans sp	1571:1613	Based on the phenotypic characteristics, chemotaxonomic data and genotypic analyses, strain LAM7117T should be classified as a novel species of genus Arthrobacter, for which the name Arthrobacter sulfonylureivorans sp.
33140141	9	35	theme	glutamic	1133:1140	arg1	acid					1142:1145	glutamic acid	1133:1145	glutamic acid	1133:1145	The cell wall peptidoglycan contained the amino acids as glycine, lysine, alanine and glutamic acid.
33140141	2	36	theme	forest	311:316	arg1	soil					318:321	birch forest soil	305:321	birch forest soil	305:321	A gram-stain positive, aerobic, motile, rod-shaped bacterium, designated strain LAM7117T, was isolated from a sulfonylurea herbicides degrading consortium enriched with birch forest soil.
33140141	9	37	theme	cell	1051:1054	arg1	peptidoglycan					1061:1073	The cell wall peptidoglycan	1047:1073	The cell wall peptidoglycan	1047:1073	The cell wall peptidoglycan contained the amino acids as glycine, lysine, alanine and glutamic acid.
33140141	3	38	theme	strain	373:378	arg1	LAM7117T					380:387	strain LAM7117T	373:387	strain LAM7117T	373:387	The optimal temperature and pH for the growth of strain LAM7117T were 35 °C and 7.5, respectively.
33140141	12	39	theme	sulfonylureivorans	1593:1610	arg1	sp					1612:1613	the name Arthrobacter sulfonylureivorans sp	1571:1613	the name Arthrobacter sulfonylureivorans sp	1571:1613	Based on the phenotypic characteristics, chemotaxonomic data and genotypic analyses, strain LAM7117T should be classified as a novel species of genus Arthrobacter, for which the name Arthrobacter sulfonylureivorans sp.
33140141	5	40	theme	highest	671:677	arg1	similarity					679:688	the highest similarity	667:688	the highest similarity	667:688	Strain LAM7117T formed a distinct phylogenetic subclade within the genus Arthrobacter in the phylogenetic trees built with 16S rRNA gene sequences and shared the highest similarity with A. crystallopoietes JCM 2522T (97.7%).
33140141	12	41	theme	strain	1482:1487	arg1	species					1530:1536	a novel species	1522:1536	a novel species	1522:1536	Based on the phenotypic characteristics, chemotaxonomic data and genotypic analyses, strain LAM7117T should be classified as a novel species of genus Arthrobacter, for which the name Arthrobacter sulfonylureivorans sp.
33140141	12	41	theme	strain	1482:1487	arg1	LAM7117T					1489:1496	strain LAM7117T	1482:1496	strain LAM7117T	1482:1496	Based on the phenotypic characteristics, chemotaxonomic data and genotypic analyses, strain LAM7117T should be classified as a novel species of genus Arthrobacter, for which the name Arthrobacter sulfonylureivorans sp.
33140141	1	42	theme	birch	117:121	arg1	soil					130:133	birch forest soil	117:133	birch forest soil	117:133	nov., isolated from a sulfonylurea herbicides degrading consortium enriched with birch forest soil.
33140141	6	43	theme	DNA-DNA	756:762	arg1	relatedness					764:774	digital DNA-DNA relatedness	748:774	digital DNA-DNA relatedness	748:774	The values of digital DNA-DNA relatedness and Avery Nucleotide Identity based on the genome sequences between LAM7117T and A. crystallopoietes JCM 2522T were 21.4 and 77.4%, respectively.
33140141	9	44	contain	contained	1075:1083	arg2	acids					1095:1099	the amino acids	1085:1099	the amino acids as glycine, lysine, alanine and glutamic acid	1085:1145	The cell wall peptidoglycan contained the amino acids as glycine, lysine, alanine and glutamic acid.
33140141	9	44	contain	contained	1075:1083	arg1	peptidoglycan					1061:1073	The cell wall peptidoglycan	1047:1073	The cell wall peptidoglycan	1047:1073	The cell wall peptidoglycan contained the amino acids as glycine, lysine, alanine and glutamic acid.
33140141	6	45	theme	genome	819:824	arg1	sequences					826:834	the genome sequences	815:834	the genome sequences between LAM7117T and A. crystallopoietes JCM 2522T	815:885	The values of digital DNA-DNA relatedness and Avery Nucleotide Identity based on the genome sequences between LAM7117T and A. crystallopoietes JCM 2522T were 21.4 and 77.4%, respectively.
33140141	11	46	theme	predominant	1333:1343	arg1	MK-8					1382:1385	MK-8	1382:1385	MK-8	1382:1385	The predominant menaquinones of strain LAM7117T were MK-8 and MK-9.
33140141	11	46	theme	predominant	1333:1343	arg1	menaquinones					1345:1356	The predominant menaquinones	1329:1356	The predominant menaquinones of strain LAM7117T	1329:1375	The predominant menaquinones of strain LAM7117T were MK-8 and MK-9.
33140141	4	47	dep	9	499:499	arg1	to					496:497	to	496:497	to	496:497	Strain LAM7117T could grow in the presence of NaCl with concentration up to 9% (w/v).
33140141	14	48	theme	32824T = CGMCC	1669:1682	arg1	LAM7117T					1653:1660	LAM7117T	1653:1660	LAM7117T (= JCM 32824T = CGMCC 1.16681T)	1653:1692	The type strain is LAM7117T (= JCM 32824T = CGMCC 1.16681T).
33140141	14	48	theme	32824T = CGMCC	1669:1682	arg1	1.16681T					1684:1691	= JCM 32824T = CGMCC 1.16681T	1663:1691	= JCM 32824T = CGMCC 1.16681T	1663:1691	The type strain is LAM7117T (= JCM 32824T = CGMCC 1.16681T).
33140141	10	49	theme	unidentified	1309:1320	arg1	lipid					1322:1326	one unidentified lipid	1305:1326	one unidentified lipid	1305:1326	The major polar lipids present in strain LAM7117T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl inositol, two unidentified glycolipids and one unidentified lipid.
33140141	12	50	theme	Arthrobacter	1547:1558	arg1	species					1530:1536	a novel species	1522:1536	a novel species	1522:1536	Based on the phenotypic characteristics, chemotaxonomic data and genotypic analyses, strain LAM7117T should be classified as a novel species of genus Arthrobacter, for which the name Arthrobacter sulfonylureivorans sp.
33140141	12	50	theme	Arthrobacter	1547:1558	arg1	LAM7117T					1489:1496	strain LAM7117T	1482:1496	strain LAM7117T	1482:1496	Based on the phenotypic characteristics, chemotaxonomic data and genotypic analyses, strain LAM7117T should be classified as a novel species of genus Arthrobacter, for which the name Arthrobacter sulfonylureivorans sp.
33140141	5	51	theme	gene	641:644	arg1	sequences					646:654	16S rRNA gene sequences	632:654	16S rRNA gene sequences	632:654	Strain LAM7117T formed a distinct phylogenetic subclade within the genus Arthrobacter in the phylogenetic trees built with 16S rRNA gene sequences and shared the highest similarity with A. crystallopoietes JCM 2522T (97.7%).
33140141	14	52	theme	= JCM	1663:1667	arg1	LAM7117T					1653:1660	LAM7117T	1653:1660	LAM7117T (= JCM 32824T = CGMCC 1.16681T)	1653:1692	The type strain is LAM7117T (= JCM 32824T = CGMCC 1.16681T).
33140141	14	52	theme	= JCM	1663:1667	arg1	1.16681T					1684:1691	= JCM 32824T = CGMCC 1.16681T	1663:1691	= JCM 32824T = CGMCC 1.16681T	1663:1691	The type strain is LAM7117T (= JCM 32824T = CGMCC 1.16681T).
33140141	5	53	theme	Strain	509:514	arg1	LAM7117T					516:523	Strain LAM7117T	509:523	Strain LAM7117T	509:523	Strain LAM7117T formed a distinct phylogenetic subclade within the genus Arthrobacter in the phylogenetic trees built with 16S rRNA gene sequences and shared the highest similarity with A. crystallopoietes JCM 2522T (97.7%).
33140141	1	54	theme	forest	123:128	arg1	soil					130:133	birch forest soil	117:133	birch forest soil	117:133	nov., isolated from a sulfonylurea herbicides degrading consortium enriched with birch forest soil.
33140141	7	55	theme	DNA	934:936	arg1	content					944:950	The genomic DNA G + C content	922:950	The genomic DNA G + C content	922:950	The genomic DNA G + C content was 65.9 mol%.
33140141	7	55	theme	DNA	934:936	arg1	%					964:964	65.9 mol%	956:964	65.9 mol%	956:964	The genomic DNA G + C content was 65.9 mol%.
33140141	14	56	theme	type	1638:1641	arg1	LAM7117T					1653:1660	LAM7117T	1653:1660	LAM7117T (= JCM 32824T = CGMCC 1.16681T)	1653:1692	The type strain is LAM7117T (= JCM 32824T = CGMCC 1.16681T).
33140141	14	56	theme	type	1638:1641	arg1	strain					1643:1648	The type strain	1634:1648	The type strain	1634:1648	The type strain is LAM7117T (= JCM 32824T = CGMCC 1.16681T).
33140141	2	57	theme	strain	209:214	arg1	LAM7117T					216:223	strain LAM7117T	209:223	strain LAM7117T	209:223	A gram-stain positive, aerobic, motile, rod-shaped bacterium, designated strain LAM7117T, was isolated from a sulfonylurea herbicides degrading consortium enriched with birch forest soil.
33140141	9	58	theme	amino	1089:1093	arg1	acids					1095:1099	the amino acids	1085:1099	the amino acids as glycine, lysine, alanine and glutamic acid	1085:1145	The cell wall peptidoglycan contained the amino acids as glycine, lysine, alanine and glutamic acid.
33140141	8	59	theme	fatty	986:990	arg1	anteiso-C15:0					1003:1015	anteiso-C15:0	1003:1015	anteiso-C15:0	1003:1015	The major cellular fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0.
33140141	8	59	theme	fatty	986:990	arg1	acids					992:996	The major cellular fatty acids	967:996	The major cellular fatty acids	967:996	The major cellular fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0.
33140141	4	60	dep	concentration	479:491	arg1	w/v					503:505	w/v	503:505	w/v	503:505	Strain LAM7117T could grow in the presence of NaCl with concentration up to 9% (w/v).
33140141	4	60	dep	concentration	479:491	arg1	%					500:500	up to 9%	493:500	concentration up to 9% (w/v)	479:506	Strain LAM7117T could grow in the presence of NaCl with concentration up to 9% (w/v).
33140141	5	61	dep	A.	695:696	arg1	crystallopoietes					698:713	crystallopoietes	698:713	crystallopoietes	698:713	Strain LAM7117T formed a distinct phylogenetic subclade within the genus Arthrobacter in the phylogenetic trees built with 16S rRNA gene sequences and shared the highest similarity with A. crystallopoietes JCM 2522T (97.7%).
33140141	6	62	theme	Identity	797:804	arg1	values					738:743	The values	734:743	The values of digital DNA-DNA relatedness and Avery Nucleotide Identity based on the genome sequences between LAM7117T and A. crystallopoietes JCM 2522T	734:885	The values of digital DNA-DNA relatedness and Avery Nucleotide Identity based on the genome sequences between LAM7117T and A. crystallopoietes JCM 2522T were 21.4 and 77.4%, respectively.
33140141	6	62	theme	Identity	797:804	arg1	%					905:905	21.4 and 77.4%	892:905	21.4 and 77.4%	892:905	The values of digital DNA-DNA relatedness and Avery Nucleotide Identity based on the genome sequences between LAM7117T and A. crystallopoietes JCM 2522T were 21.4 and 77.4%, respectively.
33140141	7	63	theme	65.9 mol	956:963	arg1	content					944:950	The genomic DNA G + C content	922:950	The genomic DNA G + C content	922:950	The genomic DNA G + C content was 65.9 mol%.
33140141	7	63	theme	65.9 mol	956:963	arg1	%					964:964	65.9 mol%	956:964	65.9 mol%	956:964	The genomic DNA G + C content was 65.9 mol%.
33140141	10	64	theme	present	1171:1177	arg1	lipids					1164:1169	The major polar lipids	1148:1169	The major polar lipids present in strain LAM7117T	1148:1196	The major polar lipids present in strain LAM7117T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl inositol, two unidentified glycolipids and one unidentified lipid.
33140141	10	64	theme	present	1171:1177	arg1	diphosphatidylglycerol					1203:1224	diphosphatidylglycerol	1203:1224	diphosphatidylglycerol	1203:1224	The major polar lipids present in strain LAM7117T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl inositol, two unidentified glycolipids and one unidentified lipid.
33140141	6	65	dep	LAM7117T	844:851	arg1	2522T					881:885	JCM 2522T	877:885	JCM 2522T	877:885	The values of digital DNA-DNA relatedness and Avery Nucleotide Identity based on the genome sequences between LAM7117T and A. crystallopoietes JCM 2522T were 21.4 and 77.4%, respectively.
33140141	10	66	from	present	1171:1177	arg1	LAM7117T					1189:1196	strain LAM7117T	1182:1196	strain LAM7117T	1182:1196	The major polar lipids present in strain LAM7117T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl inositol, two unidentified glycolipids and one unidentified lipid.
33140141	6	67	theme	Nucleotide	786:795	arg1	Identity					797:804	Avery Nucleotide Identity	780:804	Avery Nucleotide Identity	780:804	The values of digital DNA-DNA relatedness and Avery Nucleotide Identity based on the genome sequences between LAM7117T and A. crystallopoietes JCM 2522T were 21.4 and 77.4%, respectively.
33140141	0	68	dep	sp	32:33	arg1	sulfonylureivorans					13:30	Arthrobacter sulfonylureivorans	0:30	Arthrobacter sulfonylureivorans	0:30	Arthrobacter sulfonylureivorans sp.
33140141	7	69	theme	G + C	938:942	arg1	content					944:950	The genomic DNA G + C content	922:950	The genomic DNA G + C content	922:950	The genomic DNA G + C content was 65.9 mol%.
33140141	7	69	theme	G + C	938:942	arg1	%					964:964	65.9 mol%	956:964	65.9 mol%	956:964	The genomic DNA G + C content was 65.9 mol%.
33140141	12	70	theme	novel	1524:1528	arg1	species					1530:1536	a novel species	1522:1536	a novel species	1522:1536	Based on the phenotypic characteristics, chemotaxonomic data and genotypic analyses, strain LAM7117T should be classified as a novel species of genus Arthrobacter, for which the name Arthrobacter sulfonylureivorans sp.
33140141	12	70	theme	novel	1524:1528	arg1	LAM7117T					1489:1496	strain LAM7117T	1482:1496	strain LAM7117T	1482:1496	Based on the phenotypic characteristics, chemotaxonomic data and genotypic analyses, strain LAM7117T should be classified as a novel species of genus Arthrobacter, for which the name Arthrobacter sulfonylureivorans sp.
33140141	11	71	theme	strain	1361:1366	arg1	LAM7117T					1368:1375	strain LAM7117T	1361:1375	strain LAM7117T	1361:1375	The predominant menaquinones of strain LAM7117T were MK-8 and MK-9.
33140141	4	72	theme	Strain	423:428	arg1	LAM7117T					430:437	Strain LAM7117T	423:437	Strain LAM7117T	423:437	Strain LAM7117T could grow in the presence of NaCl with concentration up to 9% (w/v).
33140141	12	73	theme	name	1575:1578	arg1	sp					1612:1613	the name Arthrobacter sulfonylureivorans sp	1571:1613	the name Arthrobacter sulfonylureivorans sp	1571:1613	Based on the phenotypic characteristics, chemotaxonomic data and genotypic analyses, strain LAM7117T should be classified as a novel species of genus Arthrobacter, for which the name Arthrobacter sulfonylureivorans sp.
33140141	9	74	theme	wall	1056:1059	arg1	peptidoglycan					1061:1073	The cell wall peptidoglycan	1047:1073	The cell wall peptidoglycan	1047:1073	The cell wall peptidoglycan contained the amino acids as glycine, lysine, alanine and glutamic acid.
33140141	10	75	theme	polar	1158:1162	arg1	lipids					1164:1169	The major polar lipids	1148:1169	The major polar lipids present in strain LAM7117T	1148:1196	The major polar lipids present in strain LAM7117T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl inositol, two unidentified glycolipids and one unidentified lipid.
33140141	10	75	theme	polar	1158:1162	arg1	diphosphatidylglycerol					1203:1224	diphosphatidylglycerol	1203:1224	diphosphatidylglycerol	1203:1224	The major polar lipids present in strain LAM7117T were diphosphatidylglycerol, phosphatidylglycerol, phosphatidyl inositol, two unidentified glycolipids and one unidentified lipid.
33140141	12	76	theme	genus	1541:1545	arg1	Arthrobacter					1547:1558	genus Arthrobacter	1541:1558	genus Arthrobacter	1541:1558	Based on the phenotypic characteristics, chemotaxonomic data and genotypic analyses, strain LAM7117T should be classified as a novel species of genus Arthrobacter, for which the name Arthrobacter sulfonylureivorans sp.
33140141	11	77	theme	LAM7117T	1368:1375	arg1	MK-8					1382:1385	MK-8	1382:1385	MK-8	1382:1385	The predominant menaquinones of strain LAM7117T were MK-8 and MK-9.
33140141	11	77	theme	LAM7117T	1368:1375	arg1	menaquinones					1345:1356	The predominant menaquinones	1329:1356	The predominant menaquinones of strain LAM7117T	1329:1375	The predominant menaquinones of strain LAM7117T were MK-8 and MK-9.
33140141	12	78	theme	chemotaxonomic	1438:1451	arg1	characteristics					1421:1435	the phenotypic characteristics	1406:1435	the phenotypic characteristics	1406:1435	Based on the phenotypic characteristics, chemotaxonomic data and genotypic analyses, strain LAM7117T should be classified as a novel species of genus Arthrobacter, for which the name Arthrobacter sulfonylureivorans sp.
33140141	12	78	theme	chemotaxonomic	1438:1451	arg1	data					1453:1456	chemotaxonomic data	1438:1456	chemotaxonomic data	1438:1456	Based on the phenotypic characteristics, chemotaxonomic data and genotypic analyses, strain LAM7117T should be classified as a novel species of genus Arthrobacter, for which the name Arthrobacter sulfonylureivorans sp.
33140141	7	79	theme	genomic	926:932	arg1	content					944:950	The genomic DNA G + C content	922:950	The genomic DNA G + C content	922:950	The genomic DNA G + C content was 65.9 mol%.
33140141	7	79	theme	genomic	926:932	arg1	%					964:964	65.9 mol%	956:964	65.9 mol%	956:964	The genomic DNA G + C content was 65.9 mol%.
33140141	12	80	theme	genotypic	1462:1470	arg1	characteristics					1421:1435	the phenotypic characteristics	1406:1435	the phenotypic characteristics	1406:1435	Based on the phenotypic characteristics, chemotaxonomic data and genotypic analyses, strain LAM7117T should be classified as a novel species of genus Arthrobacter, for which the name Arthrobacter sulfonylureivorans sp.
33140141	12	80	theme	genotypic	1462:1470	arg1	analyses					1472:1479	genotypic analyses	1462:1479	genotypic analyses	1462:1479	Based on the phenotypic characteristics, chemotaxonomic data and genotypic analyses, strain LAM7117T should be classified as a novel species of genus Arthrobacter, for which the name Arthrobacter sulfonylureivorans sp.
31800390	2	0	dep	Gram-stain-positive	61:79	arg1	aerobic					91:97	aerobic	91:97	aerobic	91:97	A Gram-stain-positive, strictly aerobic, non-motile, non-spore-forming and oval-shaped bacterium, designated strain BO-16T was isolated from activated sludge.
31800390	12	1	theme	caeni	1530:1534	arg1	sp					1536:1537	the name Flexivirga caeni sp	1510:1537	the name Flexivirga caeni sp	1510:1537	Therefore the isolate represents a novel species, for which the name Flexivirga caeni sp.
31800390	4	2	theme	R2A	449:451	arg1	agar					453:456	R2A agar	449:456	R2A agar	449:456	Growth of BO-16T was observed at 10-40 °C (optimum, 25-37 °C) and at pH 5.0-10.0 (optimum, pH 7.0) on R2A agar.
31800390	2	3	theme	non-motile	100:109	arg1	bacterium					146:154	A Gram-stain-positive, strictly aerobic, non-motile, non-spore-forming and oval-shaped bacterium	59:154	A Gram-stain-positive, strictly aerobic, non-motile, non-spore-forming and oval-shaped bacterium	59:154	A Gram-stain-positive, strictly aerobic, non-motile, non-spore-forming and oval-shaped bacterium, designated strain BO-16T was isolated from activated sludge.
31800390	4	4	dep	optimum	390:396	arg1	25-37 °C					399:406	25-37 °C	399:406	25-37 °C	399:406	Growth of BO-16T was observed at 10-40 °C (optimum, 25-37 °C) and at pH 5.0-10.0 (optimum, pH 7.0) on R2A agar.
31800390	4	5	dep	optimum	429:435	arg1	pH					438:439	pH 7.0	438:443	pH 7.0	438:443	Growth of BO-16T was observed at 10-40 °C (optimum, 25-37 °C) and at pH 5.0-10.0 (optimum, pH 7.0) on R2A agar.
31800390	10	6	theme	closest	1252:1258	arg1	neighbours					1273:1282	its closest phylogenetic neighbours	1248:1282	its closest phylogenetic neighbours	1248:1282	The DNA-DNA relatedness values between BO-16T and its closest phylogenetic neighbours were much lower than 70 %.
31800390	10	7	theme	relatedness	1210:1220	arg1	lower					1294:1298	lower	1294:1298	lower	1294:1298	The DNA-DNA relatedness values between BO-16T and its closest phylogenetic neighbours were much lower than 70 %.
31800390	10	7	theme	relatedness	1210:1220	arg1	values					1222:1227	The DNA-DNA relatedness values	1198:1227	The DNA-DNA relatedness values between BO-16T and its closest phylogenetic neighbours	1198:1282	The DNA-DNA relatedness values between BO-16T and its closest phylogenetic neighbours were much lower than 70 %.
31800390	1	8	theme	activated	41:49	arg1	sludge					51:56	activated sludge	41:56	activated sludge	41:56	nov., isolated from activated sludge.
31800390	10	9	theme	DNA-DNA	1202:1208	arg1	lower					1294:1298	lower	1294:1298	lower	1294:1298	The DNA-DNA relatedness values between BO-16T and its closest phylogenetic neighbours were much lower than 70 %.
31800390	10	9	theme	DNA-DNA	1202:1208	arg1	values					1222:1227	The DNA-DNA relatedness values	1198:1227	The DNA-DNA relatedness values between BO-16T and its closest phylogenetic neighbours	1198:1282	The DNA-DNA relatedness values between BO-16T and its closest phylogenetic neighbours were much lower than 70 %.
31800390	9	10	theme	G+C	1152:1154	arg1	content					1156:1162	The G+C content	1148:1162	The G+C content of the genomic DNA	1148:1181	The G+C content of the genomic DNA was 68.0 mol%.
31800390	9	10	theme	G+C	1152:1154	arg1	%					1195:1195	68.0 mol%	1187:1195	68.0 mol%	1187:1195	The G+C content of the genomic DNA was 68.0 mol%.
31800390	7	11	contain	contained	724:732	arg2	alanine					750:756	alanine	750:756	alanine	750:756	The peptidoglycan contained lysine, serine, alanine, glycine and glutamic acid and represented the peptidoglycan type A4α.
31800390	7	11	contain	contained	724:732	arg2	lysine					734:739	lysine	734:739	lysine	734:739	The peptidoglycan contained lysine, serine, alanine, glycine and glutamic acid and represented the peptidoglycan type A4α.
31800390	7	11	contain	contained	724:732	arg2	glycine					759:765	glycine	759:765	glycine	759:765	The peptidoglycan contained lysine, serine, alanine, glycine and glutamic acid and represented the peptidoglycan type A4α.
31800390	7	11	contain	contained	724:732	arg1	peptidoglycan					710:722	The peptidoglycan	706:722	The peptidoglycan	706:722	The peptidoglycan contained lysine, serine, alanine, glycine and glutamic acid and represented the peptidoglycan type A4α.
31800390	7	11	contain	contained	724:732	arg2	acid					780:783	glutamic acid	771:783	glutamic acid	771:783	The peptidoglycan contained lysine, serine, alanine, glycine and glutamic acid and represented the peptidoglycan type A4α.
31800390	7	11	contain	contained	724:732	arg2	serine					742:747	serine	742:747	serine	742:747	The peptidoglycan contained lysine, serine, alanine, glycine and glutamic acid and represented the peptidoglycan type A4α.
31800390	11	12	theme	with	1419:1423	arg1	species					1387:1393	the species	1383:1393	the species of the genus Flexivirga with validly published names	1383:1447	BO-16T could be differentiated phylogenetically and phenotypically from the species of the genus Flexivirga with validly published names.
31800390	5	13	theme	polar	554:558	arg1	diphosphatidylglycerol					572:593	diphosphatidylglycerol	572:593	diphosphatidylglycerol	572:593	The major fatty acids it contained were iso-C16:0, anteiso-C17:0 and iso-C17 : 0 and the major polar lipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylethanolamine.
31800390	5	13	theme	polar	554:558	arg1	lipids					560:565	the major polar lipids	544:565	the major polar lipids	544:565	The major fatty acids it contained were iso-C16:0, anteiso-C17:0 and iso-C17 : 0 and the major polar lipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylethanolamine.
31800390	5	14	theme	major	548:552	arg1	diphosphatidylglycerol					572:593	diphosphatidylglycerol	572:593	diphosphatidylglycerol	572:593	The major fatty acids it contained were iso-C16:0, anteiso-C17:0 and iso-C17 : 0 and the major polar lipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylethanolamine.
31800390	5	14	theme	major	548:552	arg1	lipids					560:565	the major polar lipids	544:565	the major polar lipids	544:565	The major fatty acids it contained were iso-C16:0, anteiso-C17:0 and iso-C17 : 0 and the major polar lipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylethanolamine.
31800390	7	15	theme	peptidoglycan	805:817	arg1	A4α					824:826	the peptidoglycan type A4α	801:826	the peptidoglycan type A4α	801:826	The peptidoglycan contained lysine, serine, alanine, glycine and glutamic acid and represented the peptidoglycan type A4α.
31800390	2	16	theme	oval-shaped	134:144	arg1	bacterium					146:154	A Gram-stain-positive, strictly aerobic, non-motile, non-spore-forming and oval-shaped bacterium	59:154	A Gram-stain-positive, strictly aerobic, non-motile, non-spore-forming and oval-shaped bacterium	59:154	A Gram-stain-positive, strictly aerobic, non-motile, non-spore-forming and oval-shaped bacterium, designated strain BO-16T was isolated from activated sludge.
31800390	10	17	theme	phylogenetic	1260:1271	arg1	neighbours					1273:1282	its closest phylogenetic neighbours	1248:1282	its closest phylogenetic neighbours	1248:1282	The DNA-DNA relatedness values between BO-16T and its closest phylogenetic neighbours were much lower than 70 %.
31800390	13	18	theme	=KACC	1587:1591	arg1	BO-16T					1579:1584	the type strain BO-16T	1563:1584	the type strain BO-16T (=KACC 19647T=LMG 30859T)	1563:1610	nov. is proposed, with the type strain BO-16T (=KACC 19647T=LMG 30859T).
31800390	13	18	theme	=KACC	1587:1591	arg1	30859T					1604:1609	=KACC 19647T=LMG 30859T	1587:1609	=KACC 19647T=LMG 30859T	1587:1609	nov. is proposed, with the type strain BO-16T (=KACC 19647T=LMG 30859T).
31800390	12	19	theme	Flexivirga	1519:1528	arg1	sp					1536:1537	the name Flexivirga caeni sp	1510:1537	the name Flexivirga caeni sp	1510:1537	Therefore the isolate represents a novel species, for which the name Flexivirga caeni sp.
31800390	8	20	theme	Flexivirga	1069:1078	arg1	24460T					1089:1094	Flexivirga alba DSM 24460T	1069:1094	Flexivirga alba DSM 24460T (97.4 %)	1069:1103	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	8	20	theme	Flexivirga	1069:1078	arg1	%					1102:1102	97.4 %	1097:1102	97.4 %	1097:1102	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	8	21	theme	KCTC	1047:1050	arg1	39536T					1052:1057	Flexivirga endophytica KCTC 39536T	1024:1057	Flexivirga endophytica KCTC 39536T (97.5 %)	1024:1066	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	8	21	theme	KCTC	1047:1050	arg1	%					1065:1065	97.5 %	1060:1065	97.5 %	1060:1065	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	8	22	theme	KACC	982:985	arg1	similarity					1011:1020	98.4 % sequence similarity	995:1020	98.4 % sequence similarity	995:1020	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	8	22	theme	KACC	982:985	arg1	18597T					987:992	Flexivirga oryzae KACC 18597T	964:992	Flexivirga oryzae KACC 18597T (98.4 % sequence similarity)	964:1021	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	8	23	theme	endophytica	1035:1045	arg1	39536T					1052:1057	Flexivirga endophytica KCTC 39536T	1024:1057	Flexivirga endophytica KCTC 39536T (97.5 %)	1024:1066	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	8	23	theme	endophytica	1035:1045	arg1	%					1065:1065	97.5 %	1060:1065	97.5 %	1060:1065	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	4	24	from	10-40 °C	380:387	arg1	agar					453:456	R2A agar	449:456	R2A agar	449:456	Growth of BO-16T was observed at 10-40 °C (optimum, 25-37 °C) and at pH 5.0-10.0 (optimum, pH 7.0) on R2A agar.
31800390	12	25	theme	name	1514:1517	arg1	sp					1536:1537	the name Flexivirga caeni sp	1510:1537	the name Flexivirga caeni sp	1510:1537	Therefore the isolate represents a novel species, for which the name Flexivirga caeni sp.
31800390	2	26	attach	isolated	186:193	arg2	bacterium					146:154	A Gram-stain-positive, strictly aerobic, non-motile, non-spore-forming and oval-shaped bacterium	59:154	A Gram-stain-positive, strictly aerobic, non-motile, non-spore-forming and oval-shaped bacterium	59:154	A Gram-stain-positive, strictly aerobic, non-motile, non-spore-forming and oval-shaped bacterium, designated strain BO-16T was isolated from activated sludge.
31800390	2	26	attach	isolated	186:193	arg1	sludge					210:215	activated sludge	200:215	activated sludge	200:215	A Gram-stain-positive, strictly aerobic, non-motile, non-spore-forming and oval-shaped bacterium, designated strain BO-16T was isolated from activated sludge.
31800390	0	27	theme	Flexivirga	0:9	arg1	caeni					11:15	Flexivirga caeni	0:15	Flexivirga caeni	0:15	Flexivirga caeni sp.
31800390	9	28	theme	genomic	1171:1177	arg1	DNA					1179:1181	the genomic DNA	1167:1181	the genomic DNA	1167:1181	The G+C content of the genomic DNA was 68.0 mol%.
31800390	8	29	theme	oryzae	975:980	arg1	similarity					1011:1020	98.4 % sequence similarity	995:1020	98.4 % sequence similarity	995:1020	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	8	29	theme	oryzae	975:980	arg1	18597T					987:992	Flexivirga oryzae KACC 18597T	964:992	Flexivirga oryzae KACC 18597T (98.4 % sequence similarity)	964:1021	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	8	30	theme	Flexivirga	932:941	arg1	member					912:917	a member	910:917	a member of the genus Flexivirga	910:941	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	8	31	theme	Flexivirga	964:973	arg1	similarity					1011:1020	98.4 % sequence similarity	995:1020	98.4 % sequence similarity	995:1020	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	8	31	theme	Flexivirga	964:973	arg1	18597T					987:992	Flexivirga oryzae KACC 18597T	964:992	Flexivirga oryzae KACC 18597T (98.4 % sequence similarity)	964:1021	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	5	32	dep	acids	475:479	arg1	it					481:482	it	481:482	it	481:482	The major fatty acids it contained were iso-C16:0, anteiso-C17:0 and iso-C17 : 0 and the major polar lipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylethanolamine.
31800390	8	33	theme	Flexivirga	1109:1118	arg1	39625T					1131:1136	Flexivirga lutea KCTC 39625T	1109:1136	Flexivirga lutea KCTC 39625T (97.3 %)	1109:1145	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	8	33	theme	Flexivirga	1109:1118	arg1	%					1144:1144	97.3 %	1139:1144	97.3 %	1139:1144	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	8	34	theme	DSM	1085:1087	arg1	24460T					1089:1094	Flexivirga alba DSM 24460T	1069:1094	Flexivirga alba DSM 24460T (97.4 %)	1069:1103	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	8	34	theme	DSM	1085:1087	arg1	%					1102:1102	97.4 %	1097:1102	97.4 %	1097:1102	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	9	35	theme	DNA	1179:1181	arg1	content					1156:1162	The G+C content	1148:1162	The G+C content of the genomic DNA	1148:1181	The G+C content of the genomic DNA was 68.0 mol%.
31800390	9	35	theme	DNA	1179:1181	arg1	%					1195:1195	68.0 mol%	1187:1195	68.0 mol%	1187:1195	The G+C content of the genomic DNA was 68.0 mol%.
31800390	8	36	theme	genus	926:930	arg1	Flexivirga					932:941	the genus Flexivirga	922:941	the genus Flexivirga	922:941	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	6	37	theme	isoprenoid	652:661	arg1	quinones					663:670	This isoprenoid quinones	647:670	This isoprenoid quinones	647:670	This isoprenoid quinones included MK-8 (H4) and MK-8 (H6).
31800390	8	38	theme	alba	1080:1083	arg1	24460T					1089:1094	Flexivirga alba DSM 24460T	1069:1094	Flexivirga alba DSM 24460T (97.4 %)	1069:1103	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	8	38	theme	alba	1080:1083	arg1	%					1102:1102	97.4 %	1097:1102	97.4 %	1097:1102	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	11	39	theme	published	1433:1441	arg1	names					1443:1447	validly published names	1425:1447	the genus Flexivirga with validly published names	1398:1447	BO-16T could be differentiated phylogenetically and phenotypically from the species of the genus Flexivirga with validly published names.
31800390	8	40	theme	16S	845:847	arg1	similarity					868:877	16S rRNA gene sequence similarity	845:877	16S rRNA gene sequence similarity	845:877	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	7	41	theme	glutamic	771:778	arg1	lysine					734:739	lysine	734:739	lysine	734:739	The peptidoglycan contained lysine, serine, alanine, glycine and glutamic acid and represented the peptidoglycan type A4α.
31800390	7	41	theme	glutamic	771:778	arg1	acid					780:783	glutamic acid	771:783	glutamic acid	771:783	The peptidoglycan contained lysine, serine, alanine, glycine and glutamic acid and represented the peptidoglycan type A4α.
31800390	9	42	theme	68.0 mol	1187:1194	arg1	content					1156:1162	The G+C content	1148:1162	The G+C content of the genomic DNA	1148:1181	The G+C content of the genomic DNA was 68.0 mol%.
31800390	9	42	theme	68.0 mol	1187:1194	arg1	%					1195:1195	68.0 mol%	1187:1195	68.0 mol%	1187:1195	The G+C content of the genomic DNA was 68.0 mol%.
31800390	3	43	theme	taxonomic	249:257	arg1	characterization					259:274	characterization	259:274	characterization	259:274	In this study, we describe the taxonomic characterization and classification of this bacterium by using the polyphasic approach.
31800390	7	44	theme	type	819:822	arg1	A4α					824:826	the peptidoglycan type A4α	801:826	the peptidoglycan type A4α	801:826	The peptidoglycan contained lysine, serine, alanine, glycine and glutamic acid and represented the peptidoglycan type A4α.
31800390	4	45	located	observed	368:375	arg1	optimum					390:396	optimum	390:396	optimum	390:396	Growth of BO-16T was observed at 10-40 °C (optimum, 25-37 °C) and at pH 5.0-10.0 (optimum, pH 7.0) on R2A agar.
31800390	4	45	located	observed	368:375	arg2	Growth					347:352	Growth	347:352	Growth of BO-16T	347:362	Growth of BO-16T was observed at 10-40 °C (optimum, 25-37 °C) and at pH 5.0-10.0 (optimum, pH 7.0) on R2A agar.
31800390	4	45	located	observed	368:375	arg1	10-40 °C					380:387	10-40 °C	380:387	10-40 °C (optimum, 25-37 °C)	380:407	Growth of BO-16T was observed at 10-40 °C (optimum, 25-37 °C) and at pH 5.0-10.0 (optimum, pH 7.0) on R2A agar.
31800390	13	46	theme	type	1567:1570	arg1	BO-16T					1579:1584	the type strain BO-16T	1563:1584	the type strain BO-16T (=KACC 19647T=LMG 30859T)	1563:1610	nov. is proposed, with the type strain BO-16T (=KACC 19647T=LMG 30859T).
31800390	13	46	theme	type	1567:1570	arg1	30859T					1604:1609	=KACC 19647T=LMG 30859T	1587:1609	=KACC 19647T=LMG 30859T	1587:1609	nov. is proposed, with the type strain BO-16T (=KACC 19647T=LMG 30859T).
31800390	8	47	theme	98.4 	995:999	arg1	%					1000:1000	%	1000:1000	%	1000:1000	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	2	48	theme	strain	168:173	arg1	BO-16T					175:180	strain BO-16T	168:180	strain BO-16T	168:180	A Gram-stain-positive, strictly aerobic, non-motile, non-spore-forming and oval-shaped bacterium, designated strain BO-16T was isolated from activated sludge.
31800390	13	49	theme	19647T=LMG	1593:1602	arg1	BO-16T					1579:1584	the type strain BO-16T	1563:1584	the type strain BO-16T (=KACC 19647T=LMG 30859T)	1563:1610	nov. is proposed, with the type strain BO-16T (=KACC 19647T=LMG 30859T).
31800390	13	49	theme	19647T=LMG	1593:1602	arg1	30859T					1604:1609	=KACC 19647T=LMG 30859T	1587:1609	=KACC 19647T=LMG 30859T	1587:1609	nov. is proposed, with the type strain BO-16T (=KACC 19647T=LMG 30859T).
31800390	8	50	theme	gene	854:857	arg1	similarity					868:877	16S rRNA gene sequence similarity	845:877	16S rRNA gene sequence similarity	845:877	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	8	51	theme	KCTC	1126:1129	arg1	39625T					1131:1136	Flexivirga lutea KCTC 39625T	1109:1136	Flexivirga lutea KCTC 39625T (97.3 %)	1109:1145	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	8	51	theme	KCTC	1126:1129	arg1	%					1144:1144	97.3 %	1139:1144	97.3 %	1139:1144	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	13	52	theme	strain	1572:1577	arg1	BO-16T					1579:1584	the type strain BO-16T	1563:1584	the type strain BO-16T (=KACC 19647T=LMG 30859T)	1563:1610	nov. is proposed, with the type strain BO-16T (=KACC 19647T=LMG 30859T).
31800390	13	52	theme	strain	1572:1577	arg1	30859T					1604:1609	=KACC 19647T=LMG 30859T	1587:1609	=KACC 19647T=LMG 30859T	1587:1609	nov. is proposed, with the type strain BO-16T (=KACC 19647T=LMG 30859T).
31800390	3	53	theme	bacterium	303:311	arg1	classification					280:293	classification	280:293	classification	280:293	In this study, we describe the taxonomic characterization and classification of this bacterium by using the polyphasic approach.
31800390	3	53	theme	bacterium	303:311	arg1	characterization					259:274	characterization	259:274	characterization	259:274	In this study, we describe the taxonomic characterization and classification of this bacterium by using the polyphasic approach.
31800390	8	54	theme	Flexivirga	1024:1033	arg1	39536T					1052:1057	Flexivirga endophytica KCTC 39536T	1024:1057	Flexivirga endophytica KCTC 39536T (97.5 %)	1024:1066	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	8	54	theme	Flexivirga	1024:1033	arg1	%					1065:1065	97.5 %	1060:1065	97.5 %	1060:1065	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	8	55	theme	rRNA	849:852	arg1	similarity					868:877	16S rRNA gene sequence similarity	845:877	16S rRNA gene sequence similarity	845:877	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	8	56	theme	sequence	1002:1009	arg1	similarity					1011:1020	98.4 % sequence similarity	995:1020	98.4 % sequence similarity	995:1020	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	8	56	theme	sequence	1002:1009	arg1	18597T					987:992	Flexivirga oryzae KACC 18597T	964:992	Flexivirga oryzae KACC 18597T (98.4 % sequence similarity)	964:1021	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	4	57	theme	BO-16T	357:362	arg1	Growth					347:352	Growth	347:352	Growth of BO-16T	347:362	Growth of BO-16T was observed at 10-40 °C (optimum, 25-37 °C) and at pH 5.0-10.0 (optimum, pH 7.0) on R2A agar.
31800390	2	58	theme	Gram-stain-positive	61:79	arg1	bacterium					146:154	A Gram-stain-positive, strictly aerobic, non-motile, non-spore-forming and oval-shaped bacterium	59:154	A Gram-stain-positive, strictly aerobic, non-motile, non-spore-forming and oval-shaped bacterium	59:154	A Gram-stain-positive, strictly aerobic, non-motile, non-spore-forming and oval-shaped bacterium, designated strain BO-16T was isolated from activated sludge.
31800390	8	59	theme	%	1000:1000	arg1	similarity					1011:1020	98.4 % sequence similarity	995:1020	98.4 % sequence similarity	995:1020	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	8	59	theme	%	1000:1000	arg1	18597T					987:992	Flexivirga oryzae KACC 18597T	964:992	Flexivirga oryzae KACC 18597T (98.4 % sequence similarity)	964:1021	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	2	60	theme	activated	200:208	arg1	sludge					210:215	activated sludge	200:215	activated sludge	200:215	A Gram-stain-positive, strictly aerobic, non-motile, non-spore-forming and oval-shaped bacterium, designated strain BO-16T was isolated from activated sludge.
31800390	8	61	theme	lutea	1120:1124	arg1	39625T					1131:1136	Flexivirga lutea KCTC 39625T	1109:1136	Flexivirga lutea KCTC 39625T (97.3 %)	1109:1145	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	8	61	theme	lutea	1120:1124	arg1	%					1144:1144	97.3 %	1139:1144	97.3 %	1139:1144	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	11	62	theme	Flexivirga	1408:1417	arg1	with					1419:1423	the genus Flexivirga with validly published names	1398:1447	the genus Flexivirga with validly published names	1398:1447	BO-16T could be differentiated phylogenetically and phenotypically from the species of the genus Flexivirga with validly published names.
31800390	8	63	theme	sequence	859:866	arg1	similarity					868:877	16S rRNA gene sequence similarity	845:877	16S rRNA gene sequence similarity	845:877	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	0	64	dep	sp	17:18	arg1	caeni					11:15	Flexivirga caeni	0:15	Flexivirga caeni	0:15	Flexivirga caeni sp.
31800390	5	65	theme	major	463:467	arg1	acids					475:479	The major fatty acids	459:479	The major fatty acids it contained	459:492	The major fatty acids it contained were iso-C16:0, anteiso-C17:0 and iso-C17 : 0 and the major polar lipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylethanolamine.
31800390	5	65	theme	major	463:467	arg1	iso-C16:0					499:507	iso-C16:0	499:507	iso-C16:0	499:507	The major fatty acids it contained were iso-C16:0, anteiso-C17:0 and iso-C17 : 0 and the major polar lipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylethanolamine.
31800390	12	66	theme	novel	1485:1489	arg1	species					1491:1497	a novel species	1483:1497	a novel species	1483:1497	Therefore the isolate represents a novel species, for which the name Flexivirga caeni sp.
31800390	3	67	theme	polyphasic	326:335	arg1	approach					337:344	the polyphasic approach	322:344	the polyphasic approach	322:344	In this study, we describe the taxonomic characterization and classification of this bacterium by using the polyphasic approach.
31800390	11	68	theme	genus	1402:1406	arg1	with					1419:1423	the genus Flexivirga with validly published names	1398:1447	the genus Flexivirga with validly published names	1398:1447	BO-16T could be differentiated phylogenetically and phenotypically from the species of the genus Flexivirga with validly published names.
31800390	5	69	theme	fatty	469:473	arg1	acids					475:479	The major fatty acids	459:479	The major fatty acids it contained	459:492	The major fatty acids it contained were iso-C16:0, anteiso-C17:0 and iso-C17 : 0 and the major polar lipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylethanolamine.
31800390	5	69	theme	fatty	469:473	arg1	iso-C16:0					499:507	iso-C16:0	499:507	iso-C16:0	499:507	The major fatty acids it contained were iso-C16:0, anteiso-C17:0 and iso-C17 : 0 and the major polar lipids were diphosphatidylglycerol, phosphatidylinositol and phosphatidylethanolamine.
31800390	11	70	dep	with	1419:1423	arg1	names					1443:1447	validly published names	1425:1447	the genus Flexivirga with validly published names	1398:1447	BO-16T could be differentiated phylogenetically and phenotypically from the species of the genus Flexivirga with validly published names.
31800390	3	71	dep	characterization	259:274	arg1	the					245:247	the	245:247	the	245:247	In this study, we describe the taxonomic characterization and classification of this bacterium by using the polyphasic approach.
31800390	2	72	theme	non-spore-forming	112:128	arg1	bacterium					146:154	A Gram-stain-positive, strictly aerobic, non-motile, non-spore-forming and oval-shaped bacterium	59:154	A Gram-stain-positive, strictly aerobic, non-motile, non-spore-forming and oval-shaped bacterium	59:154	A Gram-stain-positive, strictly aerobic, non-motile, non-spore-forming and oval-shaped bacterium, designated strain BO-16T was isolated from activated sludge.
31800390	8	73	dep	similarity	868:877	arg1	basis					836:840	basis	836:840	basis	836:840	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31800390	8	73	dep	similarity	868:877	arg1	the					832:834	the	832:834	the	832:834	On the basis of 16S rRNA gene sequence similarity, BO-16T was shown to represent a member of the genus Flexivirga and to be related to Flexivirga oryzae KACC 18597T (98.4 % sequence similarity), Flexivirga endophytica KCTC 39536T (97.5 %), Flexivirga alba DSM 24460T (97.4 %) and Flexivirga lutea KCTC 39625T (97.3 %).
31613742	12	0	theme	cell-wall	1221:1229	arg1	peptidoglycan					1231:1243	cell-wall peptidoglycan	1221:1243	cell-wall peptidoglycan	1221:1243	The diagnostic amino acids of cell-wall peptidoglycan included alanine, glutamic acid, lysine and ornithine.
31613742	3	1	dep	%	334:334	arg1	w/v					337:339	w/v	337:339	w/v	337:339	The optimum growth conditions of the two novel strains were 1 % (w/v) NaCl, 37 °C and pH 7.
31613742	2	2	dep	China	265:269	arg1	PR					262:263	the Tibet-Qinghai Plateau, PR China	235:269	PR	262:263	Two previously undescribed, Gram-stain-positive, rod-shaped strains, 410T and 553, were isolated from faeces of the Tibetan antelope (Pantholops hodgsonii) from the Tibet-Qinghai Plateau, PR China.
31613742	15	3	theme	=CGMCC	1540:1545	arg1	106201T					1561:1567	=CGMCC 1.16361T= DSM 106201T	1540:1567	=CGMCC 1.16361T= DSM 106201T	1540:1567	The type strain is 410T (=CGMCC 1.16361T= DSM 106201T).
31613742	15	3	theme	=CGMCC	1540:1545	arg1	410T					1534:1537	410T	1534:1537	410T (=CGMCC 1.16361T= DSM 106201T)	1534:1568	The type strain is 410T (=CGMCC 1.16361T= DSM 106201T).
31613742	5	4	theme	phylogenomic	516:527	arg1	analyses					529:536	phylogenetic and phylogenomic analyses	499:536	phylogenetic and phylogenomic analyses	499:536	Based on results of 16S rRNA gene sequence comparison and phylogenetic and phylogenomic analyses, strains 410T and 553 were classified into the genus Actinomyces, and were closely related to Actinomyces ruminicola (97.6 %), Actinomyces oricola (93.5 %) and Actinomyces dentalis (90.8 %).
31613742	5	5	dep	Actinomyces	698:708	arg1	dentalis					710:717	Actinomyces dentalis (90.8 %)	698:726	Actinomyces dentalis (90.8 %)	698:726	Based on results of 16S rRNA gene sequence comparison and phylogenetic and phylogenomic analyses, strains 410T and 553 were classified into the genus Actinomyces, and were closely related to Actinomyces ruminicola (97.6 %), Actinomyces oricola (93.5 %) and Actinomyces dentalis (90.8 %).
31613742	2	6	dep	undescribed	89:99	arg1	rod-shaped					123:132	rod-shaped	123:132	rod-shaped	123:132	Two previously undescribed, Gram-stain-positive, rod-shaped strains, 410T and 553, were isolated from faeces of the Tibetan antelope (Pantholops hodgsonii) from the Tibet-Qinghai Plateau, PR China.
31613742	2	6	dep	undescribed	89:99	arg1	Gram-stain-positive					102:120	Gram-stain-positive	102:120	Gram-stain-positive	102:120	Two previously undescribed, Gram-stain-positive, rod-shaped strains, 410T and 553, were isolated from faeces of the Tibetan antelope (Pantholops hodgsonii) from the Tibet-Qinghai Plateau, PR China.
31613742	8	7	theme	respiratory	901:911	arg1	MK-10					927:931	MK-10	927:931	MK-10	927:931	The respiratory quinones were MK-10 (68 %) and MK-9 (32 %).
31613742	8	7	theme	respiratory	901:911	arg1	quinones					913:920	The respiratory quinones	897:920	The respiratory quinones	897:920	The respiratory quinones were MK-10 (68 %) and MK-9 (32 %).
31613742	5	8	theme	analyses	529:536	arg1	results					450:456	results	450:456	results of 16S rRNA gene sequence comparison and phylogenetic and phylogenomic analyses	450:536	Based on results of 16S rRNA gene sequence comparison and phylogenetic and phylogenomic analyses, strains 410T and 553 were classified into the genus Actinomyces, and were closely related to Actinomyces ruminicola (97.6 %), Actinomyces oricola (93.5 %) and Actinomyces dentalis (90.8 %).
31613742	13	9	theme	novel	1414:1418	arg1	species					1420:1426	a novel species	1412:1426	a novel species	1412:1426	The results of biochemical, chemotaxonomic and genotypic analyses revealed that the two novel strains represent a novel species of genus Actinomyces, for which the name Actinomyces qiguomingii sp.
31613742	3	10	theme	1 	332:333	arg1	%					334:334	%	334:334	%	334:334	The optimum growth conditions of the two novel strains were 1 % (w/v) NaCl, 37 °C and pH 7.
31613742	5	11	theme	genus	585:589	arg1	Actinomyces					591:601	the genus Actinomyces	581:601	the genus Actinomyces	581:601	Based on results of 16S rRNA gene sequence comparison and phylogenetic and phylogenomic analyses, strains 410T and 553 were classified into the genus Actinomyces, and were closely related to Actinomyces ruminicola (97.6 %), Actinomyces oricola (93.5 %) and Actinomyces dentalis (90.8 %).
31613742	7	12	theme	strain	828:833	arg1	410T					835:838	strain 410T	828:838	strain 410T	828:838	Digital DNA-DNA hybridization values between strain 410T and each of the closely related species were under 70 %.
31613742	1	13	theme	Pantholops	52:61	arg1	hodgsonii					63:71	the Pantholops hodgsonii	48:71	the Pantholops hodgsonii	48:71	nov., isolated from the Pantholops hodgsonii.
31613742	13	14	theme	novel	1388:1392	arg1	strains					1394:1400	the two novel strains	1380:1400	the two novel strains	1380:1400	The results of biochemical, chemotaxonomic and genotypic analyses revealed that the two novel strains represent a novel species of genus Actinomyces, for which the name Actinomyces qiguomingii sp.
31613742	10	15	theme	polar	1052:1056	arg1	diphosphatidylglycerol					1070:1091	diphosphatidylglycerol	1070:1091	diphosphatidylglycerol	1070:1091	The major polar lipids were diphosphatidylglycerol and phosphatidylinositol-mannoside.
31613742	10	15	theme	polar	1052:1056	arg1	lipids					1058:1063	The major polar lipids	1042:1063	The major polar lipids	1042:1063	The major polar lipids were diphosphatidylglycerol and phosphatidylinositol-mannoside.
31613742	3	16	theme	novel	313:317	arg1	strains					319:325	the two novel strains	305:325	the two novel strains	305:325	The optimum growth conditions of the two novel strains were 1 % (w/v) NaCl, 37 °C and pH 7.
31613742	4	17	theme	glucose	386:392	arg1	fermentation					394:405	glucose fermentation	386:405	glucose fermentation	386:405	The end products from glucose fermentation included ethanol and lactic acid.
31613742	11	18	theme	whole-cell	1133:1142	arg1	sugars					1144:1149	The whole-cell sugars	1129:1149	The whole-cell sugars	1129:1149	The whole-cell sugars contained rhamnose, ribose and glucose.
31613742	12	19	theme	peptidoglycan	1231:1243	arg1	acids					1212:1216	The diagnostic amino acids	1191:1216	The diagnostic amino acids of cell-wall peptidoglycan	1191:1243	The diagnostic amino acids of cell-wall peptidoglycan included alanine, glutamic acid, lysine and ornithine.
31613742	3	20	theme	strains	319:325	arg1	conditions					291:300	The optimum growth conditions	272:300	The optimum growth conditions of the two novel strains	272:325	The optimum growth conditions of the two novel strains were 1 % (w/v) NaCl, 37 °C and pH 7.
31613742	3	20	theme	strains	319:325	arg1	NaCl					342:345	1 % (w/v) NaCl	332:345	1 % (w/v) NaCl	332:345	The optimum growth conditions of the two novel strains were 1 % (w/v) NaCl, 37 °C and pH 7.
31613742	6	21	theme	strain	756:761	arg1	410T					763:766	strain 410T	756:766	strain 410T	756:766	The genomic G+C content of strain 410T was 67.4 mol%.
31613742	5	22	theme	comparison	484:493	arg1	results					450:456	results	450:456	results of 16S rRNA gene sequence comparison and phylogenetic and phylogenomic analyses	450:536	Based on results of 16S rRNA gene sequence comparison and phylogenetic and phylogenomic analyses, strains 410T and 553 were classified into the genus Actinomyces, and were closely related to Actinomyces ruminicola (97.6 %), Actinomyces oricola (93.5 %) and Actinomyces dentalis (90.8 %).
31613742	2	23	theme	Plateau	253:259	arg1	China					265:269	the Tibet-Qinghai Plateau, PR China	235:269	China	265:269	Two previously undescribed, Gram-stain-positive, rod-shaped strains, 410T and 553, were isolated from faeces of the Tibetan antelope (Pantholops hodgsonii) from the Tibet-Qinghai Plateau, PR China.
31613742	13	24	theme	biochemical	1315:1325	arg1	analyses					1357:1364	biochemical, chemotaxonomic and genotypic analyses	1315:1364	biochemical, chemotaxonomic and genotypic analyses	1315:1364	The results of biochemical, chemotaxonomic and genotypic analyses revealed that the two novel strains represent a novel species of genus Actinomyces, for which the name Actinomyces qiguomingii sp.
31613742	0	25	theme	qiguomingii	12:22	arg1	sp					24:25	Actinomyces qiguomingii sp	0:25	Actinomyces qiguomingii sp.	0:26	Actinomyces qiguomingii sp.
31613742	4	26	from	fermentation	394:405	arg1	products					372:379	The end products	364:379	The end products from glucose fermentation	364:405	The end products from glucose fermentation included ethanol and lactic acid.
31613742	6	27	theme	410T	763:766	arg1	%					780:780	67.4 mol%	772:780	67.4 mol%	772:780	The genomic G+C content of strain 410T was 67.4 mol%.
31613742	6	27	theme	410T	763:766	arg1	content					745:751	The genomic G+C content	729:751	The genomic G+C content of strain 410T	729:766	The genomic G+C content of strain 410T was 67.4 mol%.
31613742	0	28	theme	Actinomyces	0:10	arg1	sp					24:25	Actinomyces qiguomingii sp	0:25	Actinomyces qiguomingii sp.	0:26	Actinomyces qiguomingii sp.
31613742	5	29	theme	phylogenetic	499:510	arg1	analyses					529:536	phylogenetic and phylogenomic analyses	499:536	phylogenetic and phylogenomic analyses	499:536	Based on results of 16S rRNA gene sequence comparison and phylogenetic and phylogenomic analyses, strains 410T and 553 were classified into the genus Actinomyces, and were closely related to Actinomyces ruminicola (97.6 %), Actinomyces oricola (93.5 %) and Actinomyces dentalis (90.8 %).
31613742	9	30	theme	C18 	1029:1032	arg1	ω9c					1037:1039	C18 : 1 ω9c	1029:1039	C18 : 1 ω9c	1029:1039	The main cellular fatty acids of the isolates were C16 : 0, followed by C18 : 1 ω9c.
31613742	3	31	theme	%	334:334	arg1	conditions					291:300	The optimum growth conditions	272:300	The optimum growth conditions of the two novel strains	272:325	The optimum growth conditions of the two novel strains were 1 % (w/v) NaCl, 37 °C and pH 7.
31613742	3	31	theme	%	334:334	arg1	NaCl					342:345	1 % (w/v) NaCl	332:345	1 % (w/v) NaCl	332:345	The optimum growth conditions of the two novel strains were 1 % (w/v) NaCl, 37 °C and pH 7.
31613742	7	32	theme	DNA-DNA	791:797	arg1	hybridization					799:811	Digital DNA-DNA hybridization	783:811	Digital DNA-DNA hybridization values between strain 410T and each of the closely related species	783:878	Digital DNA-DNA hybridization values between strain 410T and each of the closely related species were under 70 %.
31613742	5	33	dep	Actinomyces	665:675	arg1	oricola					677:683	Actinomyces oricola (93.5 %)	665:692	Actinomyces oricola (93.5 %)	665:692	Based on results of 16S rRNA gene sequence comparison and phylogenetic and phylogenomic analyses, strains 410T and 553 were classified into the genus Actinomyces, and were closely related to Actinomyces ruminicola (97.6 %), Actinomyces oricola (93.5 %) and Actinomyces dentalis (90.8 %).
31613742	7	34	theme	hybridization	799:811	arg1	values					813:818	Digital DNA-DNA hybridization values	783:818	Digital DNA-DNA hybridization values between strain 410T and each of the closely related species	783:878	Digital DNA-DNA hybridization values between strain 410T and each of the closely related species were under 70 %.
31613742	9	35	theme	isolates	994:1001	arg1	C16 					1008:1011	C16 	1008:1011	C16 	1008:1011	The main cellular fatty acids of the isolates were C16 : 0, followed by C18 : 1 ω9c.
31613742	9	35	theme	isolates	994:1001	arg1	acids					981:985	The main cellular fatty acids	957:985	The main cellular fatty acids of the isolates	957:1001	The main cellular fatty acids of the isolates were C16 : 0, followed by C18 : 1 ω9c.
31613742	15	36	theme	type	1519:1522	arg1	410T					1534:1537	410T	1534:1537	410T (=CGMCC 1.16361T= DSM 106201T)	1534:1568	The type strain is 410T (=CGMCC 1.16361T= DSM 106201T).
31613742	15	36	theme	type	1519:1522	arg1	strain					1524:1529	The type strain	1515:1529	The type strain	1515:1529	The type strain is 410T (=CGMCC 1.16361T= DSM 106201T).
31613742	2	37	attach	isolated	162:169	arg2	553					152:154	553	152:154	553	152:154	Two previously undescribed, Gram-stain-positive, rod-shaped strains, 410T and 553, were isolated from faeces of the Tibetan antelope (Pantholops hodgsonii) from the Tibet-Qinghai Plateau, PR China.
31613742	2	37	attach	isolated	162:169	arg2	410T					143:146	410T	143:146	410T	143:146	Two previously undescribed, Gram-stain-positive, rod-shaped strains, 410T and 553, were isolated from faeces of the Tibetan antelope (Pantholops hodgsonii) from the Tibet-Qinghai Plateau, PR China.
31613742	2	37	attach	isolated	162:169	arg1	China					265:269	the Tibet-Qinghai Plateau, PR China	235:269	China	265:269	Two previously undescribed, Gram-stain-positive, rod-shaped strains, 410T and 553, were isolated from faeces of the Tibetan antelope (Pantholops hodgsonii) from the Tibet-Qinghai Plateau, PR China.
31613742	2	37	attach	isolated	162:169	arg2	strains					134:140	Two previously undescribed, Gram-stain-positive, rod-shaped strains	74:140	Two previously undescribed, Gram-stain-positive, rod-shaped strains	74:140	Two previously undescribed, Gram-stain-positive, rod-shaped strains, 410T and 553, were isolated from faeces of the Tibetan antelope (Pantholops hodgsonii) from the Tibet-Qinghai Plateau, PR China.
31613742	2	37	attach	isolated	162:169	arg1	faeces					176:181	faeces	176:181	faeces of the Tibetan antelope (Pantholops hodgsonii)	176:228	Two previously undescribed, Gram-stain-positive, rod-shaped strains, 410T and 553, were isolated from faeces of the Tibetan antelope (Pantholops hodgsonii) from the Tibet-Qinghai Plateau, PR China.
31613742	9	38	theme	fatty	975:979	arg1	C16 					1008:1011	C16 	1008:1011	C16 	1008:1011	The main cellular fatty acids of the isolates were C16 : 0, followed by C18 : 1 ω9c.
31613742	9	38	theme	fatty	975:979	arg1	acids					981:985	The main cellular fatty acids	957:985	The main cellular fatty acids of the isolates	957:1001	The main cellular fatty acids of the isolates were C16 : 0, followed by C18 : 1 ω9c.
31613742	13	39	theme	Actinomyces	1469:1479	arg1	sp					1493:1494	the name Actinomyces qiguomingii sp	1460:1494	the name Actinomyces qiguomingii sp	1460:1494	The results of biochemical, chemotaxonomic and genotypic analyses revealed that the two novel strains represent a novel species of genus Actinomyces, for which the name Actinomyces qiguomingii sp.
31613742	4	40	theme	end	368:370	arg1	products					372:379	The end products	364:379	The end products from glucose fermentation	364:405	The end products from glucose fermentation included ethanol and lactic acid.
31613742	12	41	theme	diagnostic	1195:1204	arg1	acids					1212:1216	The diagnostic amino acids	1191:1216	The diagnostic amino acids of cell-wall peptidoglycan	1191:1243	The diagnostic amino acids of cell-wall peptidoglycan included alanine, glutamic acid, lysine and ornithine.
31613742	13	42	theme	name	1464:1467	arg1	sp					1493:1494	the name Actinomyces qiguomingii sp	1460:1494	the name Actinomyces qiguomingii sp	1460:1494	The results of biochemical, chemotaxonomic and genotypic analyses revealed that the two novel strains represent a novel species of genus Actinomyces, for which the name Actinomyces qiguomingii sp.
31613742	6	43	theme	G+C	741:743	arg1	%					780:780	67.4 mol%	772:780	67.4 mol%	772:780	The genomic G+C content of strain 410T was 67.4 mol%.
31613742	6	43	theme	G+C	741:743	arg1	content					745:751	The genomic G+C content	729:751	The genomic G+C content of strain 410T	729:766	The genomic G+C content of strain 410T was 67.4 mol%.
31613742	13	44	theme	chemotaxonomic	1328:1341	arg1	analyses					1357:1364	biochemical, chemotaxonomic and genotypic analyses	1315:1364	biochemical, chemotaxonomic and genotypic analyses	1315:1364	The results of biochemical, chemotaxonomic and genotypic analyses revealed that the two novel strains represent a novel species of genus Actinomyces, for which the name Actinomyces qiguomingii sp.
31613742	12	45	theme	amino	1206:1210	arg1	acids					1212:1216	The diagnostic amino acids	1191:1216	The diagnostic amino acids of cell-wall peptidoglycan	1191:1243	The diagnostic amino acids of cell-wall peptidoglycan included alanine, glutamic acid, lysine and ornithine.
31613742	6	46	theme	genomic	733:739	arg1	%					780:780	67.4 mol%	772:780	67.4 mol%	772:780	The genomic G+C content of strain 410T was 67.4 mol%.
31613742	6	46	theme	genomic	733:739	arg1	content					745:751	The genomic G+C content	729:751	The genomic G+C content of strain 410T	729:766	The genomic G+C content of strain 410T was 67.4 mol%.
31613742	2	47	theme	Pantholops	208:217	arg1	antelope					198:205	the Tibetan antelope	186:205	the Tibetan antelope (Pantholops hodgsonii)	186:228	Two previously undescribed, Gram-stain-positive, rod-shaped strains, 410T and 553, were isolated from faeces of the Tibetan antelope (Pantholops hodgsonii) from the Tibet-Qinghai Plateau, PR China.
31613742	2	47	theme	Pantholops	208:217	arg1	hodgsonii					219:227	Pantholops hodgsonii	208:227	Pantholops hodgsonii	208:227	Two previously undescribed, Gram-stain-positive, rod-shaped strains, 410T and 553, were isolated from faeces of the Tibetan antelope (Pantholops hodgsonii) from the Tibet-Qinghai Plateau, PR China.
31613742	5	48	theme	16S	461:463	arg1	gene					470:473	16S rRNA gene	461:473	16S rRNA gene sequence comparison	461:493	Based on results of 16S rRNA gene sequence comparison and phylogenetic and phylogenomic analyses, strains 410T and 553 were classified into the genus Actinomyces, and were closely related to Actinomyces ruminicola (97.6 %), Actinomyces oricola (93.5 %) and Actinomyces dentalis (90.8 %).
31613742	12	49	theme	glutamic	1263:1270	arg1	acid					1272:1275	glutamic acid	1263:1275	glutamic acid	1263:1275	The diagnostic amino acids of cell-wall peptidoglycan included alanine, glutamic acid, lysine and ornithine.
31613742	2	50	theme	undescribed	89:99	arg1	410T					143:146	410T	143:146	410T	143:146	Two previously undescribed, Gram-stain-positive, rod-shaped strains, 410T and 553, were isolated from faeces of the Tibetan antelope (Pantholops hodgsonii) from the Tibet-Qinghai Plateau, PR China.
31613742	2	50	theme	undescribed	89:99	arg1	strains					134:140	Two previously undescribed, Gram-stain-positive, rod-shaped strains	74:140	Two previously undescribed, Gram-stain-positive, rod-shaped strains	74:140	Two previously undescribed, Gram-stain-positive, rod-shaped strains, 410T and 553, were isolated from faeces of the Tibetan antelope (Pantholops hodgsonii) from the Tibet-Qinghai Plateau, PR China.
31613742	2	50	theme	undescribed	89:99	arg1	553					152:154	553	152:154	553	152:154	Two previously undescribed, Gram-stain-positive, rod-shaped strains, 410T and 553, were isolated from faeces of the Tibetan antelope (Pantholops hodgsonii) from the Tibet-Qinghai Plateau, PR China.
31613742	3	51	theme	optimum	276:282	arg1	conditions					291:300	The optimum growth conditions	272:300	The optimum growth conditions of the two novel strains	272:325	The optimum growth conditions of the two novel strains were 1 % (w/v) NaCl, 37 °C and pH 7.
31613742	3	51	theme	optimum	276:282	arg1	NaCl					342:345	1 % (w/v) NaCl	332:345	1 % (w/v) NaCl	332:345	The optimum growth conditions of the two novel strains were 1 % (w/v) NaCl, 37 °C and pH 7.
31613742	9	52	theme	 1	1034:1035	arg1	ω9c					1037:1039	C18 : 1 ω9c	1029:1039	C18 : 1 ω9c	1029:1039	The main cellular fatty acids of the isolates were C16 : 0, followed by C18 : 1 ω9c.
31613742	5	53	theme	rRNA	465:468	arg1	gene					470:473	16S rRNA gene	461:473	16S rRNA gene sequence comparison	461:493	Based on results of 16S rRNA gene sequence comparison and phylogenetic and phylogenomic analyses, strains 410T and 553 were classified into the genus Actinomyces, and were closely related to Actinomyces ruminicola (97.6 %), Actinomyces oricola (93.5 %) and Actinomyces dentalis (90.8 %).
31613742	6	54	theme	67.4 mol	772:779	arg1	%					780:780	67.4 mol%	772:780	67.4 mol%	772:780	The genomic G+C content of strain 410T was 67.4 mol%.
31613742	6	54	theme	67.4 mol	772:779	arg1	content					745:751	The genomic G+C content	729:751	The genomic G+C content of strain 410T	729:766	The genomic G+C content of strain 410T was 67.4 mol%.
31613742	3	55	theme	growth	284:289	arg1	conditions					291:300	The optimum growth conditions	272:300	The optimum growth conditions of the two novel strains	272:325	The optimum growth conditions of the two novel strains were 1 % (w/v) NaCl, 37 °C and pH 7.
31613742	3	55	theme	growth	284:289	arg1	NaCl					342:345	1 % (w/v) NaCl	332:345	1 % (w/v) NaCl	332:345	The optimum growth conditions of the two novel strains were 1 % (w/v) NaCl, 37 °C and pH 7.
31613742	2	56	theme	Tibetan	190:196	arg1	antelope					198:205	the Tibetan antelope	186:205	the Tibetan antelope (Pantholops hodgsonii)	186:228	Two previously undescribed, Gram-stain-positive, rod-shaped strains, 410T and 553, were isolated from faeces of the Tibetan antelope (Pantholops hodgsonii) from the Tibet-Qinghai Plateau, PR China.
31613742	2	56	theme	Tibetan	190:196	arg1	hodgsonii					219:227	Pantholops hodgsonii	208:227	Pantholops hodgsonii	208:227	Two previously undescribed, Gram-stain-positive, rod-shaped strains, 410T and 553, were isolated from faeces of the Tibetan antelope (Pantholops hodgsonii) from the Tibet-Qinghai Plateau, PR China.
31613742	2	57	theme	antelope	198:205	arg1	faeces					176:181	faeces	176:181	faeces of the Tibetan antelope (Pantholops hodgsonii)	176:228	Two previously undescribed, Gram-stain-positive, rod-shaped strains, 410T and 553, were isolated from faeces of the Tibetan antelope (Pantholops hodgsonii) from the Tibet-Qinghai Plateau, PR China.
31613742	5	58	theme	gene	470:473	arg1	comparison					484:493	16S rRNA gene sequence comparison	461:493	16S rRNA gene sequence comparison	461:493	Based on results of 16S rRNA gene sequence comparison and phylogenetic and phylogenomic analyses, strains 410T and 553 were classified into the genus Actinomyces, and were closely related to Actinomyces ruminicola (97.6 %), Actinomyces oricola (93.5 %) and Actinomyces dentalis (90.8 %).
31613742	13	59	theme	Actinomyces	1437:1447	arg1	species					1420:1426	a novel species	1412:1426	a novel species	1412:1426	The results of biochemical, chemotaxonomic and genotypic analyses revealed that the two novel strains represent a novel species of genus Actinomyces, for which the name Actinomyces qiguomingii sp.
31613742	2	60	theme	Tibet-Qinghai	239:251	arg1	China					265:269	the Tibet-Qinghai Plateau, PR China	235:269	China	265:269	Two previously undescribed, Gram-stain-positive, rod-shaped strains, 410T and 553, were isolated from faeces of the Tibetan antelope (Pantholops hodgsonii) from the Tibet-Qinghai Plateau, PR China.
31613742	5	61	theme	sequence	475:482	arg1	comparison					484:493	16S rRNA gene sequence comparison	461:493	16S rRNA gene sequence comparison	461:493	Based on results of 16S rRNA gene sequence comparison and phylogenetic and phylogenomic analyses, strains 410T and 553 were classified into the genus Actinomyces, and were closely related to Actinomyces ruminicola (97.6 %), Actinomyces oricola (93.5 %) and Actinomyces dentalis (90.8 %).
31613742	7	62	theme	Digital	783:789	arg1	hybridization					799:811	Digital DNA-DNA hybridization	783:811	Digital DNA-DNA hybridization values between strain 410T and each of the closely related species	783:878	Digital DNA-DNA hybridization values between strain 410T and each of the closely related species were under 70 %.
31613742	9	63	theme	main	961:964	arg1	C16 					1008:1011	C16 	1008:1011	C16 	1008:1011	The main cellular fatty acids of the isolates were C16 : 0, followed by C18 : 1 ω9c.
31613742	9	63	theme	main	961:964	arg1	acids					981:985	The main cellular fatty acids	957:985	The main cellular fatty acids of the isolates	957:1001	The main cellular fatty acids of the isolates were C16 : 0, followed by C18 : 1 ω9c.
31613742	13	64	theme	genotypic	1347:1355	arg1	analyses					1357:1364	biochemical, chemotaxonomic and genotypic analyses	1315:1364	biochemical, chemotaxonomic and genotypic analyses	1315:1364	The results of biochemical, chemotaxonomic and genotypic analyses revealed that the two novel strains represent a novel species of genus Actinomyces, for which the name Actinomyces qiguomingii sp.
31613742	9	65	theme	cellular	966:973	arg1	C16 					1008:1011	C16 	1008:1011	C16 	1008:1011	The main cellular fatty acids of the isolates were C16 : 0, followed by C18 : 1 ω9c.
31613742	9	65	theme	cellular	966:973	arg1	acids					981:985	The main cellular fatty acids	957:985	The main cellular fatty acids of the isolates	957:1001	The main cellular fatty acids of the isolates were C16 : 0, followed by C18 : 1 ω9c.
31613742	15	66	theme	1.16361T=	1547:1555	arg1	106201T					1561:1567	=CGMCC 1.16361T= DSM 106201T	1540:1567	=CGMCC 1.16361T= DSM 106201T	1540:1567	The type strain is 410T (=CGMCC 1.16361T= DSM 106201T).
31613742	15	66	theme	1.16361T=	1547:1555	arg1	410T					1534:1537	410T	1534:1537	410T (=CGMCC 1.16361T= DSM 106201T)	1534:1568	The type strain is 410T (=CGMCC 1.16361T= DSM 106201T).
31613742	13	67	theme	genus	1431:1435	arg1	Actinomyces					1437:1447	genus Actinomyces	1431:1447	genus Actinomyces	1431:1447	The results of biochemical, chemotaxonomic and genotypic analyses revealed that the two novel strains represent a novel species of genus Actinomyces, for which the name Actinomyces qiguomingii sp.
31613742	13	68	theme	analyses	1357:1364	arg1	results					1304:1310	The results	1300:1310	The results of biochemical, chemotaxonomic and genotypic analyses	1300:1364	The results of biochemical, chemotaxonomic and genotypic analyses revealed that the two novel strains represent a novel species of genus Actinomyces, for which the name Actinomyces qiguomingii sp.
31613742	7	69	theme	related	864:870	arg1	species					872:878	the closely related species	852:878	the closely related species	852:878	Digital DNA-DNA hybridization values between strain 410T and each of the closely related species were under 70 %.
31613742	15	70	theme	DSM	1557:1559	arg1	106201T					1561:1567	=CGMCC 1.16361T= DSM 106201T	1540:1567	=CGMCC 1.16361T= DSM 106201T	1540:1567	The type strain is 410T (=CGMCC 1.16361T= DSM 106201T).
31613742	15	70	theme	DSM	1557:1559	arg1	410T					1534:1537	410T	1534:1537	410T (=CGMCC 1.16361T= DSM 106201T)	1534:1568	The type strain is 410T (=CGMCC 1.16361T= DSM 106201T).
31613742	5	71	dep	Actinomyces	632:642	arg1	ruminicola					644:653	Actinomyces ruminicola (97.6 %)	632:662	Actinomyces ruminicola (97.6 %)	632:662	Based on results of 16S rRNA gene sequence comparison and phylogenetic and phylogenomic analyses, strains 410T and 553 were classified into the genus Actinomyces, and were closely related to Actinomyces ruminicola (97.6 %), Actinomyces oricola (93.5 %) and Actinomyces dentalis (90.8 %).
31613742	4	72	theme	lactic	428:433	arg1	acid					435:438	lactic acid	428:438	lactic acid	428:438	The end products from glucose fermentation included ethanol and lactic acid.
31613742	11	73	contain	contained	1151:1159	arg2	glucose					1182:1188	glucose	1182:1188	glucose	1182:1188	The whole-cell sugars contained rhamnose, ribose and glucose.
31613742	11	73	contain	contained	1151:1159	arg2	ribose					1171:1176	ribose	1171:1176	ribose	1171:1176	The whole-cell sugars contained rhamnose, ribose and glucose.
31613742	11	73	contain	contained	1151:1159	arg2	rhamnose					1161:1168	rhamnose	1161:1168	rhamnose	1161:1168	The whole-cell sugars contained rhamnose, ribose and glucose.
31613742	11	73	contain	contained	1151:1159	arg1	sugars					1144:1149	The whole-cell sugars	1129:1149	The whole-cell sugars	1129:1149	The whole-cell sugars contained rhamnose, ribose and glucose.
31613742	10	74	theme	major	1046:1050	arg1	diphosphatidylglycerol					1070:1091	diphosphatidylglycerol	1070:1091	diphosphatidylglycerol	1070:1091	The major polar lipids were diphosphatidylglycerol and phosphatidylinositol-mannoside.
31613742	10	74	theme	major	1046:1050	arg1	lipids					1058:1063	The major polar lipids	1042:1063	The major polar lipids	1042:1063	The major polar lipids were diphosphatidylglycerol and phosphatidylinositol-mannoside.
33038510	2	0	theme	associated	530:539	arg1	structure					554:562	the tumor associated carbohydrate structure	520:562	the tumor associated carbohydrate structure sialyl-Lewis x (sLex)	520:584	Since PDA shows an altered glycan expression, here we have focused on finding novel potential biomarkers, namely glycoproteins that express the tumor associated carbohydrate structure sialyl-Lewis x (sLex), which is described in PDA.
33038510	11	1	from	glycosylation	2010:2022	arg1	PDA					2027:2029	PDA	2027:2029	PDA	2027:2029	In the present work, we aimed to shed light on novel biomarkers based on altered glycosylation in PDA, in particular those glycoproteins of PDA tissues carrying the tumor carbohydrate antigen sialyl-Lewis x (sLex).
33038510	11	1	from	glycosylation	2010:2022	arg1	particular					2035:2044	particular	2035:2044	particular	2035:2044	In the present work, we aimed to shed light on novel biomarkers based on altered glycosylation in PDA, in particular those glycoproteins of PDA tissues carrying the tumor carbohydrate antigen sialyl-Lewis x (sLex).
33038510	8	2	from	advances	1426:1433	arg1	field					1442:1446	the field	1438:1446	the field of cancer research	1438:1465	SIGNIFICANCE: Despite advances in the field of cancer research, pancreatic ductal adenocarcinoma (PDA) lacks of a specific and sensitive biomarker for its early detection, when curative resection is still possible before metastases arise.
33038510	1	3	theme	serological	359:369	arg1	markers					371:377	reliable serological markers	350:377	reliable serological markers	350:377	Late diagnosis of pancreatic ductal adenocarcinoma (PDA) is one of the reasons of its low 5-year survival rate and it is due to its unspecific symptoms during the first stages of the disease and the lack of reliable serological markers.
33038510	1	4	theme	reasons	214:220	arg1	one					203:205	one	203:205	one	203:205	Late diagnosis of pancreatic ductal adenocarcinoma (PDA) is one of the reasons of its low 5-year survival rate and it is due to its unspecific symptoms during the first stages of the disease and the lack of reliable serological markers.
33038510	1	4	theme	reasons	214:220	arg1	reasons					214:220	the reasons	210:220	the reasons of its low 5-year survival rate	210:252	Late diagnosis of pancreatic ductal adenocarcinoma (PDA) is one of the reasons of its low 5-year survival rate and it is due to its unspecific symptoms during the first stages of the disease and the lack of reliable serological markers.
33038510	11	5	theme	present	1936:1942	arg1	work					1944:1947	the present work	1932:1947	the present work	1932:1947	In the present work, we aimed to shed light on novel biomarkers based on altered glycosylation in PDA, in particular those glycoproteins of PDA tissues carrying the tumor carbohydrate antigen sialyl-Lewis x (sLex).
33038510	6	6	theme	expression	1066:1075	arg1	analysis					1048:1055	The analysis	1044:1055	The analysis of MFAP4 expression in PDA cell lines and their secretome, in combination with immunohistochemistry of pancreatic tissues,	1044:1178	The analysis of MFAP4 expression in PDA cell lines and their secretome, in combination with immunohistochemistry of pancreatic tissues, revealed that MFAP4 was not produced by PDA cells, but it was found in the pancreatic extracellular matrix.
33038510	10	7	theme	markers	1920:1926	arg1	identification					1889:1902	the identification	1885:1902	the identification of novel cancer markers	1885:1926	Glycan alterations on glycoconjugates, such as glycoproteins have emerged as a rich source for the identification of novel cancer markers.
33038510	8	8	theme	early	1559:1563	arg1	detection					1565:1573	its early detection	1555:1573	its early detection	1555:1573	SIGNIFICANCE: Despite advances in the field of cancer research, pancreatic ductal adenocarcinoma (PDA) lacks of a specific and sensitive biomarker for its early detection, when curative resection is still possible before metastases arise.
33038510	2	9	gly	glycoproteins	493:505	arg1	biomarkers					474:483	novel potential biomarkers	458:483	novel potential biomarkers	458:483	Since PDA shows an altered glycan expression, here we have focused on finding novel potential biomarkers, namely glycoproteins that express the tumor associated carbohydrate structure sialyl-Lewis x (sLex), which is described in PDA.
33038510	2	9	gly	glycoproteins	493:505	arg1	glycoproteins					493:505	glycoproteins	493:505	glycoproteins that express the tumor associated carbohydrate structure sialyl-Lewis x (sLex), which is described in PDA	493:611	Since PDA shows an altered glycan expression, here we have focused on finding novel potential biomarkers, namely glycoproteins that express the tumor associated carbohydrate structure sialyl-Lewis x (sLex), which is described in PDA.
33038510	11	10	theme	tumor	2094:2098	arg1	sLex					2137:2140	sLex	2137:2140	sLex	2137:2140	In the present work, we aimed to shed light on novel biomarkers based on altered glycosylation in PDA, in particular those glycoproteins of PDA tissues carrying the tumor carbohydrate antigen sialyl-Lewis x (sLex).
33038510	11	10	theme	tumor	2094:2098	arg1	x					2134:2134	the tumor carbohydrate antigen sialyl-Lewis x	2090:2134	the tumor carbohydrate antigen sialyl-Lewis x (sLex)	2090:2141	In the present work, we aimed to shed light on novel biomarkers based on altered glycosylation in PDA, in particular those glycoproteins of PDA tissues carrying the tumor carbohydrate antigen sialyl-Lewis x (sLex).
33038510	3	11	theme	glycoproteomic	624:637	arg1	approach					639:646	a glycoproteomic approach	622:646	a glycoproteomic approach	622:646	Through a glycoproteomic approach, we have analyzed target proteins containing sLex from PDA tissues by 2DE and immunodetection techniques, and have identified by mass spectrometry the protein MFAP4 as a carrier of sLex in PDA.
33038510	13	12	theme	extracellular	2310:2322	arg1	matrix					2324:2329	the extracellular matrix	2306:2329	the extracellular matrix in PDA	2306:2336	MFAP4 is found in the extracellular matrix in PDA and although its role in cancer progression is unclear, its sLex glycoform could be a potential biomarker in pancreatic ductal adenocarcinoma.
33038510	2	13	dep	structure	554:562	arg1	sLex					580:583	sLex	580:583	sLex	580:583	Since PDA shows an altered glycan expression, here we have focused on finding novel potential biomarkers, namely glycoproteins that express the tumor associated carbohydrate structure sialyl-Lewis x (sLex), which is described in PDA.
33038510	2	13	dep	structure	554:562	arg1	x					577:577	sialyl-Lewis x	564:577	the tumor associated carbohydrate structure sialyl-Lewis x (sLex)	520:584	Since PDA shows an altered glycan expression, here we have focused on finding novel potential biomarkers, namely glycoproteins that express the tumor associated carbohydrate structure sialyl-Lewis x (sLex), which is described in PDA.
33038510	13	14	from	role	2355:2358	arg1	progression					2370:2380	cancer progression	2363:2380	cancer progression	2363:2380	MFAP4 is found in the extracellular matrix in PDA and although its role in cancer progression is unclear, its sLex glycoform could be a potential biomarker in pancreatic ductal adenocarcinoma.
33038510	3	15	theme	target	666:671	arg1	proteins					673:680	target proteins	666:680	target proteins containing sLex from PDA tissues	666:713	Through a glycoproteomic approach, we have analyzed target proteins containing sLex from PDA tissues by 2DE and immunodetection techniques, and have identified by mass spectrometry the protein MFAP4 as a carrier of sLex in PDA.
33038510	8	16	theme	curative	1581:1588	arg1	resection					1590:1598	curative resection	1581:1598	curative resection	1581:1598	SIGNIFICANCE: Despite advances in the field of cancer research, pancreatic ductal adenocarcinoma (PDA) lacks of a specific and sensitive biomarker for its early detection, when curative resection is still possible before metastases arise.
33038510	1	17	theme	pancreatic	161:170	arg1	adenocarcinoma					179:192	pancreatic ductal adenocarcinoma	161:192	pancreatic ductal adenocarcinoma (PDA)	161:198	Late diagnosis of pancreatic ductal adenocarcinoma (PDA) is one of the reasons of its low 5-year survival rate and it is due to its unspecific symptoms during the first stages of the disease and the lack of reliable serological markers.
33038510	1	17	theme	pancreatic	161:170	arg1	PDA					195:197	PDA	195:197	PDA	195:197	Late diagnosis of pancreatic ductal adenocarcinoma (PDA) is one of the reasons of its low 5-year survival rate and it is due to its unspecific symptoms during the first stages of the disease and the lack of reliable serological markers.
33038510	6	18	located	found	1242:1246	arg1	matrix					1280:1285	the pancreatic extracellular matrix	1251:1285	the pancreatic extracellular matrix	1251:1285	The analysis of MFAP4 expression in PDA cell lines and their secretome, in combination with immunohistochemistry of pancreatic tissues, revealed that MFAP4 was not produced by PDA cells, but it was found in the pancreatic extracellular matrix.
33038510	6	18	located	found	1242:1246	arg2	it					1235:1236	it	1235:1236	it	1235:1236	The analysis of MFAP4 expression in PDA cell lines and their secretome, in combination with immunohistochemistry of pancreatic tissues, revealed that MFAP4 was not produced by PDA cells, but it was found in the pancreatic extracellular matrix.
33038510	9	19	theme	recent	1776:1781	arg1	years					1783:1787	recent years	1776:1787	recent years	1776:1787	Thus, efforts to discover new PDA biomarkers represent the first line in the fight against the increase of its incidence reported in recent years.
33038510	0	20	theme	x	104:104	arg1	protein					23:29	Microfibril associated protein 4	0:31	Microfibril associated protein 4 (MFAP4)	0:39	Microfibril associated protein 4 (MFAP4) is a carrier of the tumor associated carbohydrate sialyl-Lewis x (sLex) in pancreatic adenocarcinoma.
33038510	0	20	theme	x	104:104	arg1	carrier					46:52	a carrier	44:52	a carrier of the tumor associated carbohydrate sialyl-Lewis x (sLex) in pancreatic adenocarcinoma	44:140	Microfibril associated protein 4 (MFAP4) is a carrier of the tumor associated carbohydrate sialyl-Lewis x (sLex) in pancreatic adenocarcinoma.
33038510	5	21	theme	sLex	967:970	arg1	colocalization					949:962	the colocalization	945:962	the colocalization of sLex over MFAP4	945:981	In addition, the colocalization of sLex over MFAP4 was found only in PDA and not in control pancreatic tissues.
33038510	1	22	theme	adenocarcinoma	179:192	arg1	diagnosis					148:156	Late diagnosis	143:156	Late diagnosis of pancreatic ductal adenocarcinoma (PDA)	143:198	Late diagnosis of pancreatic ductal adenocarcinoma (PDA) is one of the reasons of its low 5-year survival rate and it is due to its unspecific symptoms during the first stages of the disease and the lack of reliable serological markers.
33038510	13	23	theme	sLex	2398:2401	arg1	biomarker					2434:2442	a potential biomarker	2422:2442	a potential biomarker in pancreatic ductal adenocarcinoma	2422:2478	MFAP4 is found in the extracellular matrix in PDA and although its role in cancer progression is unclear, its sLex glycoform could be a potential biomarker in pancreatic ductal adenocarcinoma.
33038510	13	23	theme	sLex	2398:2401	arg1	glycoform					2403:2411	its sLex glycoform	2394:2411	its sLex glycoform	2394:2411	MFAP4 is found in the extracellular matrix in PDA and although its role in cancer progression is unclear, its sLex glycoform could be a potential biomarker in pancreatic ductal adenocarcinoma.
33038510	2	24	theme	novel	458:462	arg1	biomarkers					474:483	novel potential biomarkers	458:483	novel potential biomarkers	458:483	Since PDA shows an altered glycan expression, here we have focused on finding novel potential biomarkers, namely glycoproteins that express the tumor associated carbohydrate structure sialyl-Lewis x (sLex), which is described in PDA.
33038510	2	24	theme	novel	458:462	arg1	glycoproteins					493:505	glycoproteins	493:505	glycoproteins that express the tumor associated carbohydrate structure sialyl-Lewis x (sLex), which is described in PDA	493:611	Since PDA shows an altered glycan expression, here we have focused on finding novel potential biomarkers, namely glycoproteins that express the tumor associated carbohydrate structure sialyl-Lewis x (sLex), which is described in PDA.
33038510	11	25	theme	antigen	2113:2119	arg1	sLex					2137:2140	sLex	2137:2140	sLex	2137:2140	In the present work, we aimed to shed light on novel biomarkers based on altered glycosylation in PDA, in particular those glycoproteins of PDA tissues carrying the tumor carbohydrate antigen sialyl-Lewis x (sLex).
33038510	11	25	theme	antigen	2113:2119	arg1	x					2134:2134	the tumor carbohydrate antigen sialyl-Lewis x	2090:2134	the tumor carbohydrate antigen sialyl-Lewis x (sLex)	2090:2141	In the present work, we aimed to shed light on novel biomarkers based on altered glycosylation in PDA, in particular those glycoproteins of PDA tissues carrying the tumor carbohydrate antigen sialyl-Lewis x (sLex).
33038510	1	26	theme	disease	326:332	arg1	stages					312:317	the first stages	302:317	the first stages of the disease	302:332	Late diagnosis of pancreatic ductal adenocarcinoma (PDA) is one of the reasons of its low 5-year survival rate and it is due to its unspecific symptoms during the first stages of the disease and the lack of reliable serological markers.
33038510	12	27	theme	glycoproteomic	2154:2167	arg1	approach					2169:2176	a glycoproteomic approach	2152:2176	a glycoproteomic approach	2152:2176	Through a glycoproteomic approach, we have shown that the glycoprotein MFAP4 carries sLex in PDA tissues and not in control pancreatic tissues.
33038510	9	28	dep	efforts	1649:1655	arg1	discover					1660:1667	discover	1660:1667	to discover new PDA biomarkers	1657:1686	Thus, efforts to discover new PDA biomarkers represent the first line in the fight against the increase of its incidence reported in recent years.
33038510	3	29	theme	2DE	718:720	arg1	techniques					742:751	2DE and immunodetection techniques	718:751	2DE and immunodetection techniques	718:751	Through a glycoproteomic approach, we have analyzed target proteins containing sLex from PDA tissues by 2DE and immunodetection techniques, and have identified by mass spectrometry the protein MFAP4 as a carrier of sLex in PDA.
33038510	13	30	located	found	2297:2301	arg1	matrix					2324:2329	the extracellular matrix	2306:2329	the extracellular matrix in PDA	2306:2336	MFAP4 is found in the extracellular matrix in PDA and although its role in cancer progression is unclear, its sLex glycoform could be a potential biomarker in pancreatic ductal adenocarcinoma.
33038510	13	30	located	found	2297:2301	arg2	MFAP4					2288:2292	MFAP4	2288:2292	MFAP4	2288:2292	MFAP4 is found in the extracellular matrix in PDA and although its role in cancer progression is unclear, its sLex glycoform could be a potential biomarker in pancreatic ductal adenocarcinoma.
33038510	6	31	theme	PDA	1080:1082	arg1	lines					1089:1093	PDA cell lines	1080:1093	PDA cell lines	1080:1093	The analysis of MFAP4 expression in PDA cell lines and their secretome, in combination with immunohistochemistry of pancreatic tissues, revealed that MFAP4 was not produced by PDA cells, but it was found in the pancreatic extracellular matrix.
33038510	6	32	from	secretome	1105:1113	arg1	combination					1119:1129	combination	1119:1129	combination with immunohistochemistry of pancreatic tissues	1119:1177	The analysis of MFAP4 expression in PDA cell lines and their secretome, in combination with immunohistochemistry of pancreatic tissues, revealed that MFAP4 was not produced by PDA cells, but it was found in the pancreatic extracellular matrix.
33038510	6	32	from	secretome	1105:1113	arg1	analysis					1048:1055	The analysis	1044:1055	The analysis of MFAP4 expression in PDA cell lines and their secretome, in combination with immunohistochemistry of pancreatic tissues,	1044:1178	The analysis of MFAP4 expression in PDA cell lines and their secretome, in combination with immunohistochemistry of pancreatic tissues, revealed that MFAP4 was not produced by PDA cells, but it was found in the pancreatic extracellular matrix.
33038510	13	33	theme	potential	2424:2432	arg1	biomarker					2434:2442	a potential biomarker	2422:2442	a potential biomarker in pancreatic ductal adenocarcinoma	2422:2478	MFAP4 is found in the extracellular matrix in PDA and although its role in cancer progression is unclear, its sLex glycoform could be a potential biomarker in pancreatic ductal adenocarcinoma.
33038510	13	33	theme	potential	2424:2432	arg1	glycoform					2403:2411	its sLex glycoform	2394:2411	its sLex glycoform	2394:2411	MFAP4 is found in the extracellular matrix in PDA and although its role in cancer progression is unclear, its sLex glycoform could be a potential biomarker in pancreatic ductal adenocarcinoma.
33038510	11	34	theme	novel	1976:1980	arg1	biomarkers					1982:1991	novel biomarkers	1976:1991	novel biomarkers based on altered glycosylation in PDA, in particular those glycoproteins of PDA tissues carrying the tumor carbohydrate antigen sialyl-Lewis x (sLex)	1976:2141	In the present work, we aimed to shed light on novel biomarkers based on altered glycosylation in PDA, in particular those glycoproteins of PDA tissues carrying the tumor carbohydrate antigen sialyl-Lewis x (sLex).
33038510	5	35	located	found	987:991	arg2	colocalization					949:962	the colocalization	945:962	the colocalization of sLex over MFAP4	945:981	In addition, the colocalization of sLex over MFAP4 was found only in PDA and not in control pancreatic tissues.
33038510	5	35	located	found	987:991	arg1	PDA					1001:1003	PDA	1001:1003	PDA	1001:1003	In addition, the colocalization of sLex over MFAP4 was found only in PDA and not in control pancreatic tissues.
33038510	5	35	located	found	987:991	arg1	addition					935:942	addition	935:942	addition	935:942	In addition, the colocalization of sLex over MFAP4 was found only in PDA and not in control pancreatic tissues.
33038510	3	36	theme	protein	799:805	arg1	MFAP4					807:811	the protein MFAP4	795:811	the protein MFAP4	795:811	Through a glycoproteomic approach, we have analyzed target proteins containing sLex from PDA tissues by 2DE and immunodetection techniques, and have identified by mass spectrometry the protein MFAP4 as a carrier of sLex in PDA.
33038510	1	37	theme	unspecific	275:284	arg1	symptoms					286:293	its unspecific symptoms	271:293	its unspecific symptoms during the first stages of the disease	271:332	Late diagnosis of pancreatic ductal adenocarcinoma (PDA) is one of the reasons of its low 5-year survival rate and it is due to its unspecific symptoms during the first stages of the disease and the lack of reliable serological markers.
33038510	3	38	theme	immunodetection	726:740	arg1	techniques					742:751	2DE and immunodetection techniques	718:751	2DE and immunodetection techniques	718:751	Through a glycoproteomic approach, we have analyzed target proteins containing sLex from PDA tissues by 2DE and immunodetection techniques, and have identified by mass spectrometry the protein MFAP4 as a carrier of sLex in PDA.
33038510	0	39	theme	tumor	61:65	arg1	sLex					107:110	sLex	107:110	sLex	107:110	Microfibril associated protein 4 (MFAP4) is a carrier of the tumor associated carbohydrate sialyl-Lewis x (sLex) in pancreatic adenocarcinoma.
33038510	0	39	theme	tumor	61:65	arg1	x					104:104	the tumor associated carbohydrate sialyl-Lewis x	57:104	the tumor associated carbohydrate sialyl-Lewis x (sLex)	57:111	Microfibril associated protein 4 (MFAP4) is a carrier of the tumor associated carbohydrate sialyl-Lewis x (sLex) in pancreatic adenocarcinoma.
33038510	7	40	theme	MFAP4	1307:1311	arg1	glycoform					1313:1321	MFAP4 glycoform	1307:1321	MFAP4 glycoform containing sLex in PDA tissues	1307:1352	The specificity of MFAP4 glycoform containing sLex in PDA tissues shows its relevance as a potential PDA biomarker.
33038510	10	41	gly	glycoproteins	1837:1849	arg1	glycoproteins					1837:1849	glycoproteins	1837:1849	glycoproteins	1837:1849	Glycan alterations on glycoconjugates, such as glycoproteins have emerged as a rich source for the identification of novel cancer markers.
33038510	4	42	theme	higher	857:862	arg1	expression					864:873	a higher expression	855:873	a higher expression in PDA tissues	855:888	MFAP4 showed a higher expression in PDA tissues compared with pancreatic control tissues.
33038510	6	43	from	analysis	1048:1055	arg1	lines					1089:1093	PDA cell lines	1080:1093	PDA cell lines	1080:1093	The analysis of MFAP4 expression in PDA cell lines and their secretome, in combination with immunohistochemistry of pancreatic tissues, revealed that MFAP4 was not produced by PDA cells, but it was found in the pancreatic extracellular matrix.
33038510	6	43	from	analysis	1048:1055	arg1	secretome					1105:1113	their secretome	1099:1113	their secretome	1099:1113	The analysis of MFAP4 expression in PDA cell lines and their secretome, in combination with immunohistochemistry of pancreatic tissues, revealed that MFAP4 was not produced by PDA cells, but it was found in the pancreatic extracellular matrix.
33038510	0	44	theme	carbohydrate	78:89	arg1	sLex					107:110	sLex	107:110	sLex	107:110	Microfibril associated protein 4 (MFAP4) is a carrier of the tumor associated carbohydrate sialyl-Lewis x (sLex) in pancreatic adenocarcinoma.
33038510	0	44	theme	carbohydrate	78:89	arg1	x					104:104	the tumor associated carbohydrate sialyl-Lewis x	57:104	the tumor associated carbohydrate sialyl-Lewis x (sLex)	57:111	Microfibril associated protein 4 (MFAP4) is a carrier of the tumor associated carbohydrate sialyl-Lewis x (sLex) in pancreatic adenocarcinoma.
33038510	1	45	theme	first	306:310	arg1	stages					312:317	the first stages	302:317	the first stages of the disease	302:332	Late diagnosis of pancreatic ductal adenocarcinoma (PDA) is one of the reasons of its low 5-year survival rate and it is due to its unspecific symptoms during the first stages of the disease and the lack of reliable serological markers.
33038510	9	46	theme	PDA	1673:1675	arg1	biomarkers					1677:1686	new PDA biomarkers	1669:1686	new PDA biomarkers	1669:1686	Thus, efforts to discover new PDA biomarkers represent the first line in the fight against the increase of its incidence reported in recent years.
33038510	0	47	theme	Microfibril	0:10	arg1	protein					23:29	Microfibril associated protein 4	0:31	Microfibril associated protein 4 (MFAP4)	0:39	Microfibril associated protein 4 (MFAP4) is a carrier of the tumor associated carbohydrate sialyl-Lewis x (sLex) in pancreatic adenocarcinoma.
33038510	0	47	theme	Microfibril	0:10	arg1	MFAP4					34:38	MFAP4	34:38	MFAP4	34:38	Microfibril associated protein 4 (MFAP4) is a carrier of the tumor associated carbohydrate sialyl-Lewis x (sLex) in pancreatic adenocarcinoma.
33038510	0	47	theme	Microfibril	0:10	arg1	carrier					46:52	a carrier	44:52	a carrier of the tumor associated carbohydrate sialyl-Lewis x (sLex) in pancreatic adenocarcinoma	44:140	Microfibril associated protein 4 (MFAP4) is a carrier of the tumor associated carbohydrate sialyl-Lewis x (sLex) in pancreatic adenocarcinoma.
33038510	1	48	theme	low	229:231	arg1	rate					249:252	its low 5-year survival rate	225:252	its low 5-year survival rate	225:252	Late diagnosis of pancreatic ductal adenocarcinoma (PDA) is one of the reasons of its low 5-year survival rate and it is due to its unspecific symptoms during the first stages of the disease and the lack of reliable serological markers.
33038510	3	49	from	carrier	818:824	arg1	PDA					837:839	PDA	837:839	PDA	837:839	Through a glycoproteomic approach, we have analyzed target proteins containing sLex from PDA tissues by 2DE and immunodetection techniques, and have identified by mass spectrometry the protein MFAP4 as a carrier of sLex in PDA.
33038510	13	50	theme	pancreatic	2447:2456	arg1	adenocarcinoma					2465:2478	pancreatic ductal adenocarcinoma	2447:2478	pancreatic ductal adenocarcinoma	2447:2478	MFAP4 is found in the extracellular matrix in PDA and although its role in cancer progression is unclear, its sLex glycoform could be a potential biomarker in pancreatic ductal adenocarcinoma.
33038510	1	51	theme	survival	240:247	arg1	rate					249:252	its low 5-year survival rate	225:252	its low 5-year survival rate	225:252	Late diagnosis of pancreatic ductal adenocarcinoma (PDA) is one of the reasons of its low 5-year survival rate and it is due to its unspecific symptoms during the first stages of the disease and the lack of reliable serological markers.
33038510	3	52	theme	sLex	829:832	arg1	carrier					818:824	a carrier	816:824	a carrier of sLex in PDA	816:839	Through a glycoproteomic approach, we have analyzed target proteins containing sLex from PDA tissues by 2DE and immunodetection techniques, and have identified by mass spectrometry the protein MFAP4 as a carrier of sLex in PDA.
33038510	2	53	theme	altered	399:405	arg1	expression					414:423	an altered glycan expression	396:423	an altered glycan expression	396:423	Since PDA shows an altered glycan expression, here we have focused on finding novel potential biomarkers, namely glycoproteins that express the tumor associated carbohydrate structure sialyl-Lewis x (sLex), which is described in PDA.
33038510	5	54	theme	pancreatic	1024:1033	arg1	tissues					1035:1041	control pancreatic tissues	1016:1041	control pancreatic tissues	1016:1041	In addition, the colocalization of sLex over MFAP4 was found only in PDA and not in control pancreatic tissues.
33038510	7	55	theme	PDA	1389:1391	arg1	biomarker					1393:1401	a potential PDA biomarker	1377:1401	a potential PDA biomarker	1377:1401	The specificity of MFAP4 glycoform containing sLex in PDA tissues shows its relevance as a potential PDA biomarker.
33038510	7	55	theme	PDA	1389:1391	arg1	relevance					1364:1372	its relevance	1360:1372	its relevance	1360:1372	The specificity of MFAP4 glycoform containing sLex in PDA tissues shows its relevance as a potential PDA biomarker.
33038510	4	56	theme	control	915:921	arg1	tissues					923:929	pancreatic control tissues	904:929	pancreatic control tissues	904:929	MFAP4 showed a higher expression in PDA tissues compared with pancreatic control tissues.
33038510	6	57	theme	pancreatic	1255:1264	arg1	matrix					1280:1285	the pancreatic extracellular matrix	1251:1285	the pancreatic extracellular matrix	1251:1285	The analysis of MFAP4 expression in PDA cell lines and their secretome, in combination with immunohistochemistry of pancreatic tissues, revealed that MFAP4 was not produced by PDA cells, but it was found in the pancreatic extracellular matrix.
33038510	13	58	from	biomarker	2434:2442	arg1	adenocarcinoma					2465:2478	pancreatic ductal adenocarcinoma	2447:2478	pancreatic ductal adenocarcinoma	2447:2478	MFAP4 is found in the extracellular matrix in PDA and although its role in cancer progression is unclear, its sLex glycoform could be a potential biomarker in pancreatic ductal adenocarcinoma.
33038510	9	59	theme	first	1702:1706	arg1	line					1708:1711	the first line	1698:1711	the first line	1698:1711	Thus, efforts to discover new PDA biomarkers represent the first line in the fight against the increase of its incidence reported in recent years.
33038510	8	60	theme	research	1458:1465	arg1	field					1442:1446	the field	1438:1446	the field of cancer research	1438:1465	SIGNIFICANCE: Despite advances in the field of cancer research, pancreatic ductal adenocarcinoma (PDA) lacks of a specific and sensitive biomarker for its early detection, when curative resection is still possible before metastases arise.
33038510	13	61	theme	cancer	2363:2368	arg1	progression					2370:2380	cancer progression	2363:2380	cancer progression	2363:2380	MFAP4 is found in the extracellular matrix in PDA and although its role in cancer progression is unclear, its sLex glycoform could be a potential biomarker in pancreatic ductal adenocarcinoma.
33038510	8	62	theme	pancreatic	1468:1477	arg1	PDA					1502:1504	PDA	1502:1504	PDA	1502:1504	SIGNIFICANCE: Despite advances in the field of cancer research, pancreatic ductal adenocarcinoma (PDA) lacks of a specific and sensitive biomarker for its early detection, when curative resection is still possible before metastases arise.
33038510	8	62	theme	pancreatic	1468:1477	arg1	adenocarcinoma					1486:1499	pancreatic ductal adenocarcinoma	1468:1499	pancreatic ductal adenocarcinoma (PDA)	1468:1505	SIGNIFICANCE: Despite advances in the field of cancer research, pancreatic ductal adenocarcinoma (PDA) lacks of a specific and sensitive biomarker for its early detection, when curative resection is still possible before metastases arise.
33038510	7	63	theme	PDA	1342:1344	arg1	tissues					1346:1352	PDA tissues	1342:1352	PDA tissues	1342:1352	The specificity of MFAP4 glycoform containing sLex in PDA tissues shows its relevance as a potential PDA biomarker.
33038510	2	64	theme	tumor	524:528	arg1	structure					554:562	the tumor associated carbohydrate structure	520:562	the tumor associated carbohydrate structure sialyl-Lewis x (sLex)	520:584	Since PDA shows an altered glycan expression, here we have focused on finding novel potential biomarkers, namely glycoproteins that express the tumor associated carbohydrate structure sialyl-Lewis x (sLex), which is described in PDA.
33038510	6	65	theme	pancreatic	1160:1169	arg1	tissues					1171:1177	pancreatic tissues	1160:1177	pancreatic tissues	1160:1177	The analysis of MFAP4 expression in PDA cell lines and their secretome, in combination with immunohistochemistry of pancreatic tissues, revealed that MFAP4 was not produced by PDA cells, but it was found in the pancreatic extracellular matrix.
33038510	1	66	theme	markers	371:377	arg1	lack					342:345	the lack	338:345	the lack of reliable serological markers	338:377	Late diagnosis of pancreatic ductal adenocarcinoma (PDA) is one of the reasons of its low 5-year survival rate and it is due to its unspecific symptoms during the first stages of the disease and the lack of reliable serological markers.
33038510	1	66	theme	markers	371:377	arg1	symptoms					286:293	its unspecific symptoms	271:293	its unspecific symptoms during the first stages of the disease	271:332	Late diagnosis of pancreatic ductal adenocarcinoma (PDA) is one of the reasons of its low 5-year survival rate and it is due to its unspecific symptoms during the first stages of the disease and the lack of reliable serological markers.
33038510	11	67	gly	glycoproteins	2052:2064	arg1	glycoproteins					2052:2064	those glycoproteins	2046:2064	particular those glycoproteins of PDA tissues carrying the tumor carbohydrate antigen sialyl-Lewis x (sLex)	2035:2141	In the present work, we aimed to shed light on novel biomarkers based on altered glycosylation in PDA, in particular those glycoproteins of PDA tissues carrying the tumor carbohydrate antigen sialyl-Lewis x (sLex).
33038510	6	68	theme	MFAP4	1060:1064	arg1	expression					1066:1075	MFAP4 expression	1060:1075	MFAP4 expression in PDA cell lines and their secretome, in combination with immunohistochemistry of pancreatic tissues,	1060:1178	The analysis of MFAP4 expression in PDA cell lines and their secretome, in combination with immunohistochemistry of pancreatic tissues, revealed that MFAP4 was not produced by PDA cells, but it was found in the pancreatic extracellular matrix.
33038510	1	69	theme	Late	143:146	arg1	diagnosis					148:156	Late diagnosis	143:156	Late diagnosis of pancreatic ductal adenocarcinoma (PDA)	143:198	Late diagnosis of pancreatic ductal adenocarcinoma (PDA) is one of the reasons of its low 5-year survival rate and it is due to its unspecific symptoms during the first stages of the disease and the lack of reliable serological markers.
33038510	11	70	theme	altered	2002:2008	arg1	glycosylation					2010:2022	altered glycosylation	2002:2022	altered glycosylation	2002:2022	In the present work, we aimed to shed light on novel biomarkers based on altered glycosylation in PDA, in particular those glycoproteins of PDA tissues carrying the tumor carbohydrate antigen sialyl-Lewis x (sLex).
33038510	7	71	theme	glycoform	1313:1321	arg1	specificity					1292:1302	The specificity	1288:1302	The specificity of MFAP4 glycoform containing sLex in PDA tissues	1288:1352	The specificity of MFAP4 glycoform containing sLex in PDA tissues shows its relevance as a potential PDA biomarker.
33038510	2	72	theme	sialyl-Lewis	564:575	arg1	sLex					580:583	sLex	580:583	sLex	580:583	Since PDA shows an altered glycan expression, here we have focused on finding novel potential biomarkers, namely glycoproteins that express the tumor associated carbohydrate structure sialyl-Lewis x (sLex), which is described in PDA.
33038510	2	72	theme	sialyl-Lewis	564:575	arg1	x					577:577	sialyl-Lewis x	564:577	the tumor associated carbohydrate structure sialyl-Lewis x (sLex)	520:584	Since PDA shows an altered glycan expression, here we have focused on finding novel potential biomarkers, namely glycoproteins that express the tumor associated carbohydrate structure sialyl-Lewis x (sLex), which is described in PDA.
33038510	9	73	theme	incidence	1754:1762	arg1	increase					1738:1745	the increase	1734:1745	the increase of its incidence reported in recent years	1734:1787	Thus, efforts to discover new PDA biomarkers represent the first line in the fight against the increase of its incidence reported in recent years.
33038510	12	74	theme	PDA	2237:2239	arg1	tissues					2241:2247	PDA tissues	2237:2247	PDA tissues	2237:2247	Through a glycoproteomic approach, we have shown that the glycoprotein MFAP4 carries sLex in PDA tissues and not in control pancreatic tissues.
33038510	4	75	theme	PDA	878:880	arg1	tissues					882:888	PDA tissues	878:888	PDA tissues	878:888	MFAP4 showed a higher expression in PDA tissues compared with pancreatic control tissues.
33038510	13	76	from	matrix	2324:2329	arg1	PDA					2334:2336	PDA	2334:2336	PDA	2334:2336	MFAP4 is found in the extracellular matrix in PDA and although its role in cancer progression is unclear, its sLex glycoform could be a potential biomarker in pancreatic ductal adenocarcinoma.
33038510	2	77	theme	carbohydrate	541:552	arg1	structure					554:562	the tumor associated carbohydrate structure	520:562	the tumor associated carbohydrate structure sialyl-Lewis x (sLex)	520:584	Since PDA shows an altered glycan expression, here we have focused on finding novel potential biomarkers, namely glycoproteins that express the tumor associated carbohydrate structure sialyl-Lewis x (sLex), which is described in PDA.
33038510	11	78	theme	sialyl-Lewis	2121:2132	arg1	sLex					2137:2140	sLex	2137:2140	sLex	2137:2140	In the present work, we aimed to shed light on novel biomarkers based on altered glycosylation in PDA, in particular those glycoproteins of PDA tissues carrying the tumor carbohydrate antigen sialyl-Lewis x (sLex).
33038510	11	78	theme	sialyl-Lewis	2121:2132	arg1	x					2134:2134	the tumor carbohydrate antigen sialyl-Lewis x	2090:2134	the tumor carbohydrate antigen sialyl-Lewis x (sLex)	2090:2141	In the present work, we aimed to shed light on novel biomarkers based on altered glycosylation in PDA, in particular those glycoproteins of PDA tissues carrying the tumor carbohydrate antigen sialyl-Lewis x (sLex).
33038510	6	79	theme	PDA	1220:1222	arg1	cells					1224:1228	PDA cells	1220:1228	PDA cells	1220:1228	The analysis of MFAP4 expression in PDA cell lines and their secretome, in combination with immunohistochemistry of pancreatic tissues, revealed that MFAP4 was not produced by PDA cells, but it was found in the pancreatic extracellular matrix.
33038510	13	80	theme	ductal	2458:2463	arg1	adenocarcinoma					2465:2478	pancreatic ductal adenocarcinoma	2447:2478	pancreatic ductal adenocarcinoma	2447:2478	MFAP4 is found in the extracellular matrix in PDA and although its role in cancer progression is unclear, its sLex glycoform could be a potential biomarker in pancreatic ductal adenocarcinoma.
33038510	1	81	theme	ductal	172:177	arg1	adenocarcinoma					179:192	pancreatic ductal adenocarcinoma	161:192	pancreatic ductal adenocarcinoma (PDA)	161:198	Late diagnosis of pancreatic ductal adenocarcinoma (PDA) is one of the reasons of its low 5-year survival rate and it is due to its unspecific symptoms during the first stages of the disease and the lack of reliable serological markers.
33038510	1	81	theme	ductal	172:177	arg1	PDA					195:197	PDA	195:197	PDA	195:197	Late diagnosis of pancreatic ductal adenocarcinoma (PDA) is one of the reasons of its low 5-year survival rate and it is due to its unspecific symptoms during the first stages of the disease and the lack of reliable serological markers.
33038510	2	82	theme	potential	464:472	arg1	biomarkers					474:483	novel potential biomarkers	458:483	novel potential biomarkers	458:483	Since PDA shows an altered glycan expression, here we have focused on finding novel potential biomarkers, namely glycoproteins that express the tumor associated carbohydrate structure sialyl-Lewis x (sLex), which is described in PDA.
33038510	2	82	theme	potential	464:472	arg1	glycoproteins					493:505	glycoproteins	493:505	glycoproteins that express the tumor associated carbohydrate structure sialyl-Lewis x (sLex), which is described in PDA	493:611	Since PDA shows an altered glycan expression, here we have focused on finding novel potential biomarkers, namely glycoproteins that express the tumor associated carbohydrate structure sialyl-Lewis x (sLex), which is described in PDA.
33038510	11	83	theme	carbohydrate	2100:2111	arg1	sLex					2137:2140	sLex	2137:2140	sLex	2137:2140	In the present work, we aimed to shed light on novel biomarkers based on altered glycosylation in PDA, in particular those glycoproteins of PDA tissues carrying the tumor carbohydrate antigen sialyl-Lewis x (sLex).
33038510	11	83	theme	carbohydrate	2100:2111	arg1	x					2134:2134	the tumor carbohydrate antigen sialyl-Lewis x	2090:2134	the tumor carbohydrate antigen sialyl-Lewis x (sLex)	2090:2141	In the present work, we aimed to shed light on novel biomarkers based on altered glycosylation in PDA, in particular those glycoproteins of PDA tissues carrying the tumor carbohydrate antigen sialyl-Lewis x (sLex).
33038510	12	84	theme	pancreatic	2268:2277	arg1	tissues					2279:2285	control pancreatic tissues	2260:2285	control pancreatic tissues	2260:2285	Through a glycoproteomic approach, we have shown that the glycoprotein MFAP4 carries sLex in PDA tissues and not in control pancreatic tissues.
33038510	10	85	theme	Glycan	1790:1795	arg1	alterations					1797:1807	Glycan alterations	1790:1807	Glycan alterations on glycoconjugates, such as glycoproteins	1790:1849	Glycan alterations on glycoconjugates, such as glycoproteins have emerged as a rich source for the identification of novel cancer markers.
33038510	10	86	theme	cancer	1913:1918	arg1	markers					1920:1926	novel cancer markers	1907:1926	novel cancer markers	1907:1926	Glycan alterations on glycoconjugates, such as glycoproteins have emerged as a rich source for the identification of novel cancer markers.
33038510	0	87	from	carrier	46:52	arg1	adenocarcinoma					127:140	pancreatic adenocarcinoma	116:140	pancreatic adenocarcinoma	116:140	Microfibril associated protein 4 (MFAP4) is a carrier of the tumor associated carbohydrate sialyl-Lewis x (sLex) in pancreatic adenocarcinoma.
33038510	3	88	theme	PDA	703:705	arg1	tissues					707:713	PDA tissues	703:713	PDA tissues	703:713	Through a glycoproteomic approach, we have analyzed target proteins containing sLex from PDA tissues by 2DE and immunodetection techniques, and have identified by mass spectrometry the protein MFAP4 as a carrier of sLex in PDA.
33038510	0	89	theme	pancreatic	116:125	arg1	adenocarcinoma					127:140	pancreatic adenocarcinoma	116:140	pancreatic adenocarcinoma	116:140	Microfibril associated protein 4 (MFAP4) is a carrier of the tumor associated carbohydrate sialyl-Lewis x (sLex) in pancreatic adenocarcinoma.
33038510	7	90	contain	containing	1323:1332	arg1	glycoform					1313:1321	MFAP4 glycoform	1307:1321	MFAP4 glycoform containing sLex in PDA tissues	1307:1352	The specificity of MFAP4 glycoform containing sLex in PDA tissues shows its relevance as a potential PDA biomarker.
33038510	7	90	contain	containing	1323:1332	arg2	sLex					1334:1337	sLex	1334:1337	sLex	1334:1337	The specificity of MFAP4 glycoform containing sLex in PDA tissues shows its relevance as a potential PDA biomarker.
33038510	12	91	contain	carries	2221:2227	arg2	sLex					2229:2232	sLex	2229:2232	sLex in PDA tissues and not in control pancreatic tissues	2229:2285	Through a glycoproteomic approach, we have shown that the glycoprotein MFAP4 carries sLex in PDA tissues and not in control pancreatic tissues.
33038510	12	91	contain	carries	2221:2227	arg1	MFAP4					2215:2219	the glycoprotein MFAP4	2198:2219	the glycoprotein MFAP4	2198:2219	Through a glycoproteomic approach, we have shown that the glycoprotein MFAP4 carries sLex in PDA tissues and not in control pancreatic tissues.
33038510	12	92	from	sLex	2229:2232	arg1	tissues					2279:2285	control pancreatic tissues	2260:2285	control pancreatic tissues	2260:2285	Through a glycoproteomic approach, we have shown that the glycoprotein MFAP4 carries sLex in PDA tissues and not in control pancreatic tissues.
33038510	12	92	from	sLex	2229:2232	arg1	tissues					2241:2247	PDA tissues	2237:2247	PDA tissues	2237:2247	Through a glycoproteomic approach, we have shown that the glycoprotein MFAP4 carries sLex in PDA tissues and not in control pancreatic tissues.
33038510	3	93	theme	mass	777:780	arg1	spectrometry					782:793	mass spectrometry	777:793	mass spectrometry	777:793	Through a glycoproteomic approach, we have analyzed target proteins containing sLex from PDA tissues by 2DE and immunodetection techniques, and have identified by mass spectrometry the protein MFAP4 as a carrier of sLex in PDA.
33038510	6	94	theme	cell	1084:1087	arg1	lines					1089:1093	PDA cell lines	1080:1093	PDA cell lines	1080:1093	The analysis of MFAP4 expression in PDA cell lines and their secretome, in combination with immunohistochemistry of pancreatic tissues, revealed that MFAP4 was not produced by PDA cells, but it was found in the pancreatic extracellular matrix.
33038510	13	95	gly	glycoform	2403:2411	arg1	sLex					2398:2401	its sLex glycoform	2394:2411	its sLex glycoform	2394:2411	MFAP4 is found in the extracellular matrix in PDA and although its role in cancer progression is unclear, its sLex glycoform could be a potential biomarker in pancreatic ductal adenocarcinoma.
33038510	12	96	theme	control	2260:2266	arg1	tissues					2279:2285	control pancreatic tissues	2260:2285	control pancreatic tissues	2260:2285	Through a glycoproteomic approach, we have shown that the glycoprotein MFAP4 carries sLex in PDA tissues and not in control pancreatic tissues.
33038510	1	97	theme	reliable	350:357	arg1	markers					371:377	reliable serological markers	350:377	reliable serological markers	350:377	Late diagnosis of pancreatic ductal adenocarcinoma (PDA) is one of the reasons of its low 5-year survival rate and it is due to its unspecific symptoms during the first stages of the disease and the lack of reliable serological markers.
33038510	10	98	from	alterations	1797:1807	arg1	glycoconjugates					1812:1826	glycoconjugates	1812:1826	glycoconjugates	1812:1826	Glycan alterations on glycoconjugates, such as glycoproteins have emerged as a rich source for the identification of novel cancer markers.
33038510	10	98	from	alterations	1797:1807	arg1	glycoproteins					1837:1849	glycoproteins	1837:1849	glycoproteins	1837:1849	Glycan alterations on glycoconjugates, such as glycoproteins have emerged as a rich source for the identification of novel cancer markers.
33038510	2	99	theme	glycan	407:412	arg1	expression					414:423	an altered glycan expression	396:423	an altered glycan expression	396:423	Since PDA shows an altered glycan expression, here we have focused on finding novel potential biomarkers, namely glycoproteins that express the tumor associated carbohydrate structure sialyl-Lewis x (sLex), which is described in PDA.
33038510	6	100	from	lines	1089:1093	arg1	combination					1119:1129	combination	1119:1129	combination with immunohistochemistry of pancreatic tissues	1119:1177	The analysis of MFAP4 expression in PDA cell lines and their secretome, in combination with immunohistochemistry of pancreatic tissues, revealed that MFAP4 was not produced by PDA cells, but it was found in the pancreatic extracellular matrix.
33038510	6	100	from	lines	1089:1093	arg1	analysis					1048:1055	The analysis	1044:1055	The analysis of MFAP4 expression in PDA cell lines and their secretome, in combination with immunohistochemistry of pancreatic tissues,	1044:1178	The analysis of MFAP4 expression in PDA cell lines and their secretome, in combination with immunohistochemistry of pancreatic tissues, revealed that MFAP4 was not produced by PDA cells, but it was found in the pancreatic extracellular matrix.
33038510	0	101	theme	associated	67:76	arg1	sLex					107:110	sLex	107:110	sLex	107:110	Microfibril associated protein 4 (MFAP4) is a carrier of the tumor associated carbohydrate sialyl-Lewis x (sLex) in pancreatic adenocarcinoma.
33038510	0	101	theme	associated	67:76	arg1	x					104:104	the tumor associated carbohydrate sialyl-Lewis x	57:104	the tumor associated carbohydrate sialyl-Lewis x (sLex)	57:111	Microfibril associated protein 4 (MFAP4) is a carrier of the tumor associated carbohydrate sialyl-Lewis x (sLex) in pancreatic adenocarcinoma.
33038510	5	102	theme	control	1016:1022	arg1	tissues					1035:1041	control pancreatic tissues	1016:1041	control pancreatic tissues	1016:1041	In addition, the colocalization of sLex over MFAP4 was found only in PDA and not in control pancreatic tissues.
33038510	11	103	theme	PDA	2069:2071	arg1	tissues					2073:2079	PDA tissues	2069:2079	PDA tissues carrying the tumor carbohydrate antigen sialyl-Lewis x (sLex)	2069:2141	In the present work, we aimed to shed light on novel biomarkers based on altered glycosylation in PDA, in particular those glycoproteins of PDA tissues carrying the tumor carbohydrate antigen sialyl-Lewis x (sLex).
33038510	11	104	contain	carrying	2081:2088	arg2	x					2134:2134	the tumor carbohydrate antigen sialyl-Lewis x	2090:2134	the tumor carbohydrate antigen sialyl-Lewis x (sLex)	2090:2141	In the present work, we aimed to shed light on novel biomarkers based on altered glycosylation in PDA, in particular those glycoproteins of PDA tissues carrying the tumor carbohydrate antigen sialyl-Lewis x (sLex).
33038510	11	104	contain	carrying	2081:2088	arg1	tissues					2073:2079	PDA tissues	2069:2079	PDA tissues carrying the tumor carbohydrate antigen sialyl-Lewis x (sLex)	2069:2141	In the present work, we aimed to shed light on novel biomarkers based on altered glycosylation in PDA, in particular those glycoproteins of PDA tissues carrying the tumor carbohydrate antigen sialyl-Lewis x (sLex).
33038510	11	104	contain	carrying	2081:2088	arg2	sLex					2137:2140	sLex	2137:2140	sLex	2137:2140	In the present work, we aimed to shed light on novel biomarkers based on altered glycosylation in PDA, in particular those glycoproteins of PDA tissues carrying the tumor carbohydrate antigen sialyl-Lewis x (sLex).
33038510	3	105	contain	containing	682:691	arg2	sLex					693:696	sLex	693:696	sLex	693:696	Through a glycoproteomic approach, we have analyzed target proteins containing sLex from PDA tissues by 2DE and immunodetection techniques, and have identified by mass spectrometry the protein MFAP4 as a carrier of sLex in PDA.
33038510	3	105	contain	containing	682:691	arg1	proteins					673:680	target proteins	666:680	target proteins containing sLex from PDA tissues	666:713	Through a glycoproteomic approach, we have analyzed target proteins containing sLex from PDA tissues by 2DE and immunodetection techniques, and have identified by mass spectrometry the protein MFAP4 as a carrier of sLex in PDA.
33038510	10	106	theme	rich	1869:1872	arg1	source					1874:1879	a rich source	1867:1879	a rich source for the identification of novel cancer markers	1867:1926	Glycan alterations on glycoconjugates, such as glycoproteins have emerged as a rich source for the identification of novel cancer markers.
33038510	0	107	theme	sialyl-Lewis	91:102	arg1	sLex					107:110	sLex	107:110	sLex	107:110	Microfibril associated protein 4 (MFAP4) is a carrier of the tumor associated carbohydrate sialyl-Lewis x (sLex) in pancreatic adenocarcinoma.
33038510	0	107	theme	sialyl-Lewis	91:102	arg1	x					104:104	the tumor associated carbohydrate sialyl-Lewis x	57:104	the tumor associated carbohydrate sialyl-Lewis x (sLex)	57:111	Microfibril associated protein 4 (MFAP4) is a carrier of the tumor associated carbohydrate sialyl-Lewis x (sLex) in pancreatic adenocarcinoma.
33038510	6	108	from	expression	1066:1075	arg1	lines					1089:1093	PDA cell lines	1080:1093	PDA cell lines	1080:1093	The analysis of MFAP4 expression in PDA cell lines and their secretome, in combination with immunohistochemistry of pancreatic tissues, revealed that MFAP4 was not produced by PDA cells, but it was found in the pancreatic extracellular matrix.
33038510	6	108	from	expression	1066:1075	arg1	secretome					1105:1113	their secretome	1099:1113	their secretome	1099:1113	The analysis of MFAP4 expression in PDA cell lines and their secretome, in combination with immunohistochemistry of pancreatic tissues, revealed that MFAP4 was not produced by PDA cells, but it was found in the pancreatic extracellular matrix.
33038510	0	109	theme	associated	12:21	arg1	protein					23:29	Microfibril associated protein 4	0:31	Microfibril associated protein 4 (MFAP4)	0:39	Microfibril associated protein 4 (MFAP4) is a carrier of the tumor associated carbohydrate sialyl-Lewis x (sLex) in pancreatic adenocarcinoma.
33038510	0	109	theme	associated	12:21	arg1	MFAP4					34:38	MFAP4	34:38	MFAP4	34:38	Microfibril associated protein 4 (MFAP4) is a carrier of the tumor associated carbohydrate sialyl-Lewis x (sLex) in pancreatic adenocarcinoma.
33038510	0	109	theme	associated	12:21	arg1	carrier					46:52	a carrier	44:52	a carrier of the tumor associated carbohydrate sialyl-Lewis x (sLex) in pancreatic adenocarcinoma	44:140	Microfibril associated protein 4 (MFAP4) is a carrier of the tumor associated carbohydrate sialyl-Lewis x (sLex) in pancreatic adenocarcinoma.
33038510	7	110	gly	glycoform	1313:1321	arg1	MFAP4					1307:1311	MFAP4 glycoform	1307:1321	MFAP4 glycoform containing sLex in PDA tissues	1307:1352	The specificity of MFAP4 glycoform containing sLex in PDA tissues shows its relevance as a potential PDA biomarker.
33038510	1	111	theme	5-year	233:238	arg1	rate					249:252	its low 5-year survival rate	225:252	its low 5-year survival rate	225:252	Late diagnosis of pancreatic ductal adenocarcinoma (PDA) is one of the reasons of its low 5-year survival rate and it is due to its unspecific symptoms during the first stages of the disease and the lack of reliable serological markers.
33038510	3	112	dep	MFAP4	807:811	arg1	carrier					818:824	a carrier	816:824	a carrier of sLex in PDA	816:839	Through a glycoproteomic approach, we have analyzed target proteins containing sLex from PDA tissues by 2DE and immunodetection techniques, and have identified by mass spectrometry the protein MFAP4 as a carrier of sLex in PDA.
33038510	9	113	theme	new	1669:1671	arg1	biomarkers					1677:1686	new PDA biomarkers	1669:1686	new PDA biomarkers	1669:1686	Thus, efforts to discover new PDA biomarkers represent the first line in the fight against the increase of its incidence reported in recent years.
33038510	1	114	theme	rate	249:252	arg1	reasons					214:220	the reasons	210:220	the reasons of its low 5-year survival rate	210:252	Late diagnosis of pancreatic ductal adenocarcinoma (PDA) is one of the reasons of its low 5-year survival rate and it is due to its unspecific symptoms during the first stages of the disease and the lack of reliable serological markers.
33038510	11	115	dep	particular	2035:2044	arg1	glycoproteins					2052:2064	those glycoproteins	2046:2064	particular those glycoproteins of PDA tissues carrying the tumor carbohydrate antigen sialyl-Lewis x (sLex)	2035:2141	In the present work, we aimed to shed light on novel biomarkers based on altered glycosylation in PDA, in particular those glycoproteins of PDA tissues carrying the tumor carbohydrate antigen sialyl-Lewis x (sLex).
33038510	6	116	theme	extracellular	1266:1278	arg1	matrix					1280:1285	the pancreatic extracellular matrix	1251:1285	the pancreatic extracellular matrix	1251:1285	The analysis of MFAP4 expression in PDA cell lines and their secretome, in combination with immunohistochemistry of pancreatic tissues, revealed that MFAP4 was not produced by PDA cells, but it was found in the pancreatic extracellular matrix.
33038510	12	117	gly	glycoprotein	2202:2213	arg1	glycoprotein					2202:2213	the glycoprotein MFAP4	2198:2219	the glycoprotein MFAP4	2198:2219	Through a glycoproteomic approach, we have shown that the glycoprotein MFAP4 carries sLex in PDA tissues and not in control pancreatic tissues.
33038510	11	118	gly	glycosylation	2010:2022	arg1	PDA					2027:2029	PDA	2027:2029	PDA	2027:2029	In the present work, we aimed to shed light on novel biomarkers based on altered glycosylation in PDA, in particular those glycoproteins of PDA tissues carrying the tumor carbohydrate antigen sialyl-Lewis x (sLex).
33038510	11	118	gly	glycosylation	2010:2022	arg1	particular					2035:2044	particular	2035:2044	particular	2035:2044	In the present work, we aimed to shed light on novel biomarkers based on altered glycosylation in PDA, in particular those glycoproteins of PDA tissues carrying the tumor carbohydrate antigen sialyl-Lewis x (sLex).
33038510	4	119	theme	pancreatic	904:913	arg1	tissues					923:929	pancreatic control tissues	904:929	pancreatic control tissues	904:929	MFAP4 showed a higher expression in PDA tissues compared with pancreatic control tissues.
33038510	8	120	theme	cancer	1451:1456	arg1	research					1458:1465	cancer research	1451:1465	cancer research	1451:1465	SIGNIFICANCE: Despite advances in the field of cancer research, pancreatic ductal adenocarcinoma (PDA) lacks of a specific and sensitive biomarker for its early detection, when curative resection is still possible before metastases arise.
33038510	6	121	theme	tissues	1171:1177	arg1	immunohistochemistry					1136:1155	immunohistochemistry	1136:1155	immunohistochemistry of pancreatic tissues	1136:1177	The analysis of MFAP4 expression in PDA cell lines and their secretome, in combination with immunohistochemistry of pancreatic tissues, revealed that MFAP4 was not produced by PDA cells, but it was found in the pancreatic extracellular matrix.
33038510	7	122	theme	potential	1379:1387	arg1	biomarker					1393:1401	a potential PDA biomarker	1377:1401	a potential PDA biomarker	1377:1401	The specificity of MFAP4 glycoform containing sLex in PDA tissues shows its relevance as a potential PDA biomarker.
33038510	7	122	theme	potential	1379:1387	arg1	relevance					1364:1372	its relevance	1360:1372	its relevance	1360:1372	The specificity of MFAP4 glycoform containing sLex in PDA tissues shows its relevance as a potential PDA biomarker.
33038510	10	123	theme	novel	1907:1911	arg1	markers					1920:1926	novel cancer markers	1907:1926	novel cancer markers	1907:1926	Glycan alterations on glycoconjugates, such as glycoproteins have emerged as a rich source for the identification of novel cancer markers.
33038510	11	124	theme	tissues	2073:2079	arg1	glycoproteins					2052:2064	those glycoproteins	2046:2064	particular those glycoproteins of PDA tissues carrying the tumor carbohydrate antigen sialyl-Lewis x (sLex)	2035:2141	In the present work, we aimed to shed light on novel biomarkers based on altered glycosylation in PDA, in particular those glycoproteins of PDA tissues carrying the tumor carbohydrate antigen sialyl-Lewis x (sLex).
33038510	8	125	theme	specific	1518:1525	arg1	biomarker					1541:1549	a specific and sensitive biomarker	1516:1549	a specific and sensitive biomarker for its early detection	1516:1573	SIGNIFICANCE: Despite advances in the field of cancer research, pancreatic ductal adenocarcinoma (PDA) lacks of a specific and sensitive biomarker for its early detection, when curative resection is still possible before metastases arise.
33038510	6	126	with	combination	1119:1129	arg1	immunohistochemistry					1136:1155	immunohistochemistry	1136:1155	immunohistochemistry of pancreatic tissues	1136:1177	The analysis of MFAP4 expression in PDA cell lines and their secretome, in combination with immunohistochemistry of pancreatic tissues, revealed that MFAP4 was not produced by PDA cells, but it was found in the pancreatic extracellular matrix.
33038510	8	127	theme	ductal	1479:1484	arg1	PDA					1502:1504	PDA	1502:1504	PDA	1502:1504	SIGNIFICANCE: Despite advances in the field of cancer research, pancreatic ductal adenocarcinoma (PDA) lacks of a specific and sensitive biomarker for its early detection, when curative resection is still possible before metastases arise.
33038510	8	127	theme	ductal	1479:1484	arg1	adenocarcinoma					1486:1499	pancreatic ductal adenocarcinoma	1468:1499	pancreatic ductal adenocarcinoma (PDA)	1468:1505	SIGNIFICANCE: Despite advances in the field of cancer research, pancreatic ductal adenocarcinoma (PDA) lacks of a specific and sensitive biomarker for its early detection, when curative resection is still possible before metastases arise.
33038510	12	128	theme	glycoprotein	2202:2213	arg1	MFAP4					2215:2219	the glycoprotein MFAP4	2198:2219	the glycoprotein MFAP4	2198:2219	Through a glycoproteomic approach, we have shown that the glycoprotein MFAP4 carries sLex in PDA tissues and not in control pancreatic tissues.
33038510	8	129	theme	sensitive	1531:1539	arg1	biomarker					1541:1549	a specific and sensitive biomarker	1516:1549	a specific and sensitive biomarker for its early detection	1516:1573	SIGNIFICANCE: Despite advances in the field of cancer research, pancreatic ductal adenocarcinoma (PDA) lacks of a specific and sensitive biomarker for its early detection, when curative resection is still possible before metastases arise.
33038510	4	130	from	expression	864:873	arg1	tissues					882:888	PDA tissues	878:888	PDA tissues	878:888	MFAP4 showed a higher expression in PDA tissues compared with pancreatic control tissues.
31910709	3	0	theme	diabetes	679:686	arg1	model					663:667	a murine model	654:667	a murine model of type 2 diabetes (db/db) and phenotypically normal littermates (db/+)	654:739	We investigated the effects of metabolic endotoxemia after experimental stroke with transient middle cerebral artery occlusion (MCAO) in a murine model of type 2 diabetes (db/db) and phenotypically normal littermates (db/+).
31910709	6	1	theme	db/db	1267:1271	arg1	mice					1273:1276	db/db mice	1267:1276	db/db mice	1267:1276	Oral administration of a non-absorbable antibiotic modulated the gut microbiota and improved metabolic endotoxemia and stroke outcomes in db/db mice; these effects were associated with reduction of LPS levels and neuroinflammation in the ischemic brain.
31910709	5	2	from	volumes	941:947	arg1	brain					1035:1039	the ischemic brain	1022:1039	the ischemic brain	1022:1039	In addition, db/db mice presented increased infarct volumes and higher expression levels of LPS, TLR4, and inflammatory cytokines in the ischemic brain, as well as more severe neurological impairments and reduced survival rates after MCAO.
31910709	4	3	theme	higher	863:868	arg1	levels					881:886	higher plasma LPS levels	863:886	higher plasma LPS levels	863:886	Compared to db/+ mice, db/db mice exhibited an altered gut microbial composition, increased intestinal permeability, and higher plasma LPS levels.
31910709	7	4	theme	metabolic	1417:1425	arg1	endotoxemia					1427:1437	metabolic endotoxemia	1417:1437	metabolic endotoxemia	1417:1437	These data suggest that targeting metabolic endotoxemia may be a novel potential therapeutic strategy to improve stroke outcomes.
31910709	3	5	theme	normal	715:720	arg1	db/+					735:738	db/+	735:738	db/+	735:738	We investigated the effects of metabolic endotoxemia after experimental stroke with transient middle cerebral artery occlusion (MCAO) in a murine model of type 2 diabetes (db/db) and phenotypically normal littermates (db/+).
31910709	3	5	theme	normal	715:720	arg1	littermates					722:732	phenotypically normal littermates	700:732	phenotypically normal littermates (db/+)	700:739	We investigated the effects of metabolic endotoxemia after experimental stroke with transient middle cerebral artery occlusion (MCAO) in a murine model of type 2 diabetes (db/db) and phenotypically normal littermates (db/+).
31910709	3	6	theme	metabolic	548:556	arg1	endotoxemia					558:568	metabolic endotoxemia	548:568	metabolic endotoxemia	548:568	We investigated the effects of metabolic endotoxemia after experimental stroke with transient middle cerebral artery occlusion (MCAO) in a murine model of type 2 diabetes (db/db) and phenotypically normal littermates (db/+).
31910709	7	7	theme	novel	1448:1452	arg1	strategy					1476:1483	a novel potential therapeutic strategy	1446:1483	a novel potential therapeutic strategy to improve stroke outcomes	1446:1510	These data suggest that targeting metabolic endotoxemia may be a novel potential therapeutic strategy to improve stroke outcomes.
31910709	5	8	from	levels	971:976	arg1	brain					1035:1039	the ischemic brain	1022:1039	the ischemic brain	1022:1039	In addition, db/db mice presented increased infarct volumes and higher expression levels of LPS, TLR4, and inflammatory cytokines in the ischemic brain, as well as more severe neurological impairments and reduced survival rates after MCAO.
31910709	4	9	theme	intestinal	834:843	arg1	permeability					845:856	increased intestinal permeability	824:856	increased intestinal permeability	824:856	Compared to db/+ mice, db/db mice exhibited an altered gut microbial composition, increased intestinal permeability, and higher plasma LPS levels.
31910709	6	10	theme	metabolic	1222:1230	arg1	endotoxemia					1232:1242	metabolic endotoxemia	1222:1242	metabolic endotoxemia	1222:1242	Oral administration of a non-absorbable antibiotic modulated the gut microbiota and improved metabolic endotoxemia and stroke outcomes in db/db mice; these effects were associated with reduction of LPS levels and neuroinflammation in the ischemic brain.
31910709	1	11	theme	inflammatory	187:198	arg1	stimulus					200:207	a potent inflammatory stimulus	178:207	a potent inflammatory stimulus for the innate immune response	178:238	Lipopolysaccharide (LPS) is a major component of the outer membrane of Gram-negative bacteria and a potent inflammatory stimulus for the innate immune response via toll-like receptor (TLR) 4 activation.
31910709	3	12	theme	cerebral	618:625	arg1	occlusion					634:642	transient middle cerebral artery occlusion	601:642	transient middle cerebral artery occlusion (MCAO)	601:649	We investigated the effects of metabolic endotoxemia after experimental stroke with transient middle cerebral artery occlusion (MCAO) in a murine model of type 2 diabetes (db/db) and phenotypically normal littermates (db/+).
31910709	3	12	theme	cerebral	618:625	arg1	MCAO					645:648	MCAO	645:648	MCAO	645:648	We investigated the effects of metabolic endotoxemia after experimental stroke with transient middle cerebral artery occlusion (MCAO) in a murine model of type 2 diabetes (db/db) and phenotypically normal littermates (db/+).
31910709	1	13	theme	innate	217:222	arg1	response					231:238	the innate immune response	213:238	the innate immune response	213:238	Lipopolysaccharide (LPS) is a major component of the outer membrane of Gram-negative bacteria and a potent inflammatory stimulus for the innate immune response via toll-like receptor (TLR) 4 activation.
31910709	4	14	theme	LPS	877:879	arg1	levels					881:886	higher plasma LPS levels	863:886	higher plasma LPS levels	863:886	Compared to db/+ mice, db/db mice exhibited an altered gut microbial composition, increased intestinal permeability, and higher plasma LPS levels.
31910709	5	15	theme	cytokines	1009:1017	arg1	rates					1111:1115	reduced survival rates	1094:1115	reduced survival rates	1094:1115	In addition, db/db mice presented increased infarct volumes and higher expression levels of LPS, TLR4, and inflammatory cytokines in the ischemic brain, as well as more severe neurological impairments and reduced survival rates after MCAO.
31910709	5	15	theme	cytokines	1009:1017	arg1	levels					971:976	higher expression levels	953:976	higher expression levels of LPS, TLR4, and inflammatory cytokines	953:1017	In addition, db/db mice presented increased infarct volumes and higher expression levels of LPS, TLR4, and inflammatory cytokines in the ischemic brain, as well as more severe neurological impairments and reduced survival rates after MCAO.
31910709	5	15	theme	cytokines	1009:1017	arg1	impairments					1078:1088	more severe neurological impairments	1053:1088	more severe neurological impairments	1053:1088	In addition, db/db mice presented increased infarct volumes and higher expression levels of LPS, TLR4, and inflammatory cytokines in the ischemic brain, as well as more severe neurological impairments and reduced survival rates after MCAO.
31910709	5	15	theme	cytokines	1009:1017	arg1	volumes					941:947	increased infarct volumes	923:947	increased infarct volumes	923:947	In addition, db/db mice presented increased infarct volumes and higher expression levels of LPS, TLR4, and inflammatory cytokines in the ischemic brain, as well as more severe neurological impairments and reduced survival rates after MCAO.
31910709	3	16	theme	transient	601:609	arg1	occlusion					634:642	transient middle cerebral artery occlusion	601:642	transient middle cerebral artery occlusion (MCAO)	601:649	We investigated the effects of metabolic endotoxemia after experimental stroke with transient middle cerebral artery occlusion (MCAO) in a murine model of type 2 diabetes (db/db) and phenotypically normal littermates (db/+).
31910709	3	16	theme	transient	601:609	arg1	MCAO					645:648	MCAO	645:648	MCAO	645:648	We investigated the effects of metabolic endotoxemia after experimental stroke with transient middle cerebral artery occlusion (MCAO) in a murine model of type 2 diabetes (db/db) and phenotypically normal littermates (db/+).
31910709	5	17	theme	db/db	902:906	arg1	mice					908:911	db/db mice	902:911	db/db mice	902:911	In addition, db/db mice presented increased infarct volumes and higher expression levels of LPS, TLR4, and inflammatory cytokines in the ischemic brain, as well as more severe neurological impairments and reduced survival rates after MCAO.
31910709	2	18	theme	metabolic	494:502	arg1	endotoxemia					504:514	metabolic endotoxemia	494:514	metabolic endotoxemia	494:514	Type 2 diabetes is associated with changes in gut microbiota and impaired intestinal barrier functions, leading to translocation of microbiota-derived LPS into the circulatory system, a condition referred to as metabolic endotoxemia.
31910709	6	19	theme	antibiotic	1169:1178	arg1	administration					1134:1147	Oral administration	1129:1147	Oral administration of a non-absorbable antibiotic	1129:1178	Oral administration of a non-absorbable antibiotic modulated the gut microbiota and improved metabolic endotoxemia and stroke outcomes in db/db mice; these effects were associated with reduction of LPS levels and neuroinflammation in the ischemic brain.
31910709	6	20	theme	ischemic	1367:1374	arg1	brain					1376:1380	the ischemic brain	1363:1380	the ischemic brain	1363:1380	Oral administration of a non-absorbable antibiotic modulated the gut microbiota and improved metabolic endotoxemia and stroke outcomes in db/db mice; these effects were associated with reduction of LPS levels and neuroinflammation in the ischemic brain.
31910709	5	21	theme	reduced	1094:1100	arg1	rates					1111:1115	reduced survival rates	1094:1115	reduced survival rates	1094:1115	In addition, db/db mice presented increased infarct volumes and higher expression levels of LPS, TLR4, and inflammatory cytokines in the ischemic brain, as well as more severe neurological impairments and reduced survival rates after MCAO.
31910709	1	22	theme	bacteria	165:172	arg1	membrane					139:146	the outer membrane	129:146	the outer membrane of Gram-negative bacteria	129:172	Lipopolysaccharide (LPS) is a major component of the outer membrane of Gram-negative bacteria and a potent inflammatory stimulus for the innate immune response via toll-like receptor (TLR) 4 activation.
31910709	1	23	theme	major	110:114	arg1	component					116:124	a major component	108:124	a major component of the outer membrane of Gram-negative bacteria	108:172	Lipopolysaccharide (LPS) is a major component of the outer membrane of Gram-negative bacteria and a potent inflammatory stimulus for the innate immune response via toll-like receptor (TLR) 4 activation.
31910709	1	23	theme	major	110:114	arg1	Lipopolysaccharide					80:97	Lipopolysaccharide	80:97	Lipopolysaccharide (LPS)	80:103	Lipopolysaccharide (LPS) is a major component of the outer membrane of Gram-negative bacteria and a potent inflammatory stimulus for the innate immune response via toll-like receptor (TLR) 4 activation.
31910709	1	24	theme	outer	133:137	arg1	membrane					139:146	the outer membrane	129:146	the outer membrane of Gram-negative bacteria	129:172	Lipopolysaccharide (LPS) is a major component of the outer membrane of Gram-negative bacteria and a potent inflammatory stimulus for the innate immune response via toll-like receptor (TLR) 4 activation.
31910709	6	25	theme	LPS	1327:1329	arg1	levels					1331:1336	LPS levels	1327:1336	LPS levels	1327:1336	Oral administration of a non-absorbable antibiotic modulated the gut microbiota and improved metabolic endotoxemia and stroke outcomes in db/db mice; these effects were associated with reduction of LPS levels and neuroinflammation in the ischemic brain.
31910709	5	26	theme	severe	1058:1063	arg1	impairments					1078:1088	more severe neurological impairments	1053:1088	more severe neurological impairments	1053:1088	In addition, db/db mice presented increased infarct volumes and higher expression levels of LPS, TLR4, and inflammatory cytokines in the ischemic brain, as well as more severe neurological impairments and reduced survival rates after MCAO.
31910709	5	27	theme	expression	960:969	arg1	levels					971:976	higher expression levels	953:976	higher expression levels of LPS, TLR4, and inflammatory cytokines	953:1017	In addition, db/db mice presented increased infarct volumes and higher expression levels of LPS, TLR4, and inflammatory cytokines in the ischemic brain, as well as more severe neurological impairments and reduced survival rates after MCAO.
31910709	2	28	theme	circulatory	447:457	arg1	system					459:464	the circulatory system	443:464	the circulatory system	443:464	Type 2 diabetes is associated with changes in gut microbiota and impaired intestinal barrier functions, leading to translocation of microbiota-derived LPS into the circulatory system, a condition referred to as metabolic endotoxemia.
31910709	2	28	theme	circulatory	447:457	arg1	condition					469:477	a condition	467:477	a condition referred to as metabolic endotoxemia	467:514	Type 2 diabetes is associated with changes in gut microbiota and impaired intestinal barrier functions, leading to translocation of microbiota-derived LPS into the circulatory system, a condition referred to as metabolic endotoxemia.
31910709	0	29	theme	Metabolic	0:8	arg1	endotoxemia					10:20	Metabolic endotoxemia	0:20	Metabolic endotoxemia	0:20	Metabolic endotoxemia promotes neuroinflammation after focal cerebral ischemia.
31910709	7	30	dep	potential	1454:1462	arg1	therapeutic					1464:1474	therapeutic	1464:1474	therapeutic	1464:1474	These data suggest that targeting metabolic endotoxemia may be a novel potential therapeutic strategy to improve stroke outcomes.
31910709	2	31	theme	microbiota-derived	415:432	arg1	LPS					434:436	microbiota-derived LPS	415:436	microbiota-derived LPS	415:436	Type 2 diabetes is associated with changes in gut microbiota and impaired intestinal barrier functions, leading to translocation of microbiota-derived LPS into the circulatory system, a condition referred to as metabolic endotoxemia.
31910709	3	32	with	stroke	589:594	arg1	occlusion					634:642	transient middle cerebral artery occlusion	601:642	transient middle cerebral artery occlusion (MCAO)	601:649	We investigated the effects of metabolic endotoxemia after experimental stroke with transient middle cerebral artery occlusion (MCAO) in a murine model of type 2 diabetes (db/db) and phenotypically normal littermates (db/+).
31910709	3	32	with	stroke	589:594	arg1	MCAO					645:648	MCAO	645:648	MCAO	645:648	We investigated the effects of metabolic endotoxemia after experimental stroke with transient middle cerebral artery occlusion (MCAO) in a murine model of type 2 diabetes (db/db) and phenotypically normal littermates (db/+).
31910709	5	33	theme	infarct	933:939	arg1	volumes					941:947	increased infarct volumes	923:947	increased infarct volumes	923:947	In addition, db/db mice presented increased infarct volumes and higher expression levels of LPS, TLR4, and inflammatory cytokines in the ischemic brain, as well as more severe neurological impairments and reduced survival rates after MCAO.
31910709	3	34	theme	murine	656:661	arg1	model					663:667	a murine model	654:667	a murine model of type 2 diabetes (db/db) and phenotypically normal littermates (db/+)	654:739	We investigated the effects of metabolic endotoxemia after experimental stroke with transient middle cerebral artery occlusion (MCAO) in a murine model of type 2 diabetes (db/db) and phenotypically normal littermates (db/+).
31910709	4	35	theme	altered	789:795	arg1	composition					811:821	an altered gut microbial composition	786:821	an altered gut microbial composition	786:821	Compared to db/+ mice, db/db mice exhibited an altered gut microbial composition, increased intestinal permeability, and higher plasma LPS levels.
31910709	4	36	theme	microbial	801:809	arg1	composition					811:821	an altered gut microbial composition	786:821	an altered gut microbial composition	786:821	Compared to db/+ mice, db/db mice exhibited an altered gut microbial composition, increased intestinal permeability, and higher plasma LPS levels.
31910709	2	37	theme	gut	329:331	arg1	microbiota					333:342	gut microbiota	329:342	gut microbiota	329:342	Type 2 diabetes is associated with changes in gut microbiota and impaired intestinal barrier functions, leading to translocation of microbiota-derived LPS into the circulatory system, a condition referred to as metabolic endotoxemia.
31910709	0	38	theme	cerebral	61:68	arg1	ischemia					70:77	focal cerebral ischemia	55:77	focal cerebral ischemia	55:77	Metabolic endotoxemia promotes neuroinflammation after focal cerebral ischemia.
31910709	5	39	theme	inflammatory	996:1007	arg1	cytokines					1009:1017	inflammatory cytokines	996:1017	inflammatory cytokines	996:1017	In addition, db/db mice presented increased infarct volumes and higher expression levels of LPS, TLR4, and inflammatory cytokines in the ischemic brain, as well as more severe neurological impairments and reduced survival rates after MCAO.
31910709	4	40	theme	db/db	765:769	arg1	mice					771:774	db/db mice	765:774	db/db mice	765:774	Compared to db/+ mice, db/db mice exhibited an altered gut microbial composition, increased intestinal permeability, and higher plasma LPS levels.
31910709	1	41	theme	immune	224:229	arg1	response					231:238	the innate immune response	213:238	the innate immune response	213:238	Lipopolysaccharide (LPS) is a major component of the outer membrane of Gram-negative bacteria and a potent inflammatory stimulus for the innate immune response via toll-like receptor (TLR) 4 activation.
31910709	2	42	theme	intestinal	357:366	arg1	functions					376:384	impaired intestinal barrier functions	348:384	impaired intestinal barrier functions	348:384	Type 2 diabetes is associated with changes in gut microbiota and impaired intestinal barrier functions, leading to translocation of microbiota-derived LPS into the circulatory system, a condition referred to as metabolic endotoxemia.
31910709	1	43	theme	receptor	254:261	arg1	TLR					264:266	toll-like receptor (TLR) 4 activation	244:280	toll-like receptor (TLR) 4 activation	244:280	Lipopolysaccharide (LPS) is a major component of the outer membrane of Gram-negative bacteria and a potent inflammatory stimulus for the innate immune response via toll-like receptor (TLR) 4 activation.
31910709	7	44	theme	potential	1454:1462	arg1	strategy					1476:1483	a novel potential therapeutic strategy	1446:1483	a novel potential therapeutic strategy to improve stroke outcomes	1446:1510	These data suggest that targeting metabolic endotoxemia may be a novel potential therapeutic strategy to improve stroke outcomes.
31910709	2	45	from	changes	318:324	arg1	functions					376:384	impaired intestinal barrier functions	348:384	impaired intestinal barrier functions	348:384	Type 2 diabetes is associated with changes in gut microbiota and impaired intestinal barrier functions, leading to translocation of microbiota-derived LPS into the circulatory system, a condition referred to as metabolic endotoxemia.
31910709	2	45	from	changes	318:324	arg1	microbiota					333:342	gut microbiota	329:342	gut microbiota	329:342	Type 2 diabetes is associated with changes in gut microbiota and impaired intestinal barrier functions, leading to translocation of microbiota-derived LPS into the circulatory system, a condition referred to as metabolic endotoxemia.
31910709	5	46	from	rates	1111:1115	arg1	brain					1035:1039	the ischemic brain	1022:1039	the ischemic brain	1022:1039	In addition, db/db mice presented increased infarct volumes and higher expression levels of LPS, TLR4, and inflammatory cytokines in the ischemic brain, as well as more severe neurological impairments and reduced survival rates after MCAO.
31910709	2	47	theme	Type	283:286	arg1	diabetes					290:297	Type 2 diabetes	283:297	Type 2 diabetes	283:297	Type 2 diabetes is associated with changes in gut microbiota and impaired intestinal barrier functions, leading to translocation of microbiota-derived LPS into the circulatory system, a condition referred to as metabolic endotoxemia.
31910709	3	48	theme	littermates	722:732	arg1	model					663:667	a murine model	654:667	a murine model of type 2 diabetes (db/db) and phenotypically normal littermates (db/+)	654:739	We investigated the effects of metabolic endotoxemia after experimental stroke with transient middle cerebral artery occlusion (MCAO) in a murine model of type 2 diabetes (db/db) and phenotypically normal littermates (db/+).
31910709	6	49	theme	stroke	1248:1253	arg1	outcomes					1255:1262	metabolic endotoxemia and stroke outcomes	1222:1262	metabolic endotoxemia and stroke outcomes in db/db mice	1222:1276	Oral administration of a non-absorbable antibiotic modulated the gut microbiota and improved metabolic endotoxemia and stroke outcomes in db/db mice; these effects were associated with reduction of LPS levels and neuroinflammation in the ischemic brain.
31910709	6	50	theme	Oral	1129:1132	arg1	administration					1134:1147	Oral administration	1129:1147	Oral administration of a non-absorbable antibiotic	1129:1178	Oral administration of a non-absorbable antibiotic modulated the gut microbiota and improved metabolic endotoxemia and stroke outcomes in db/db mice; these effects were associated with reduction of LPS levels and neuroinflammation in the ischemic brain.
31910709	6	51	theme	endotoxemia	1232:1242	arg1	outcomes					1255:1262	metabolic endotoxemia and stroke outcomes	1222:1262	metabolic endotoxemia and stroke outcomes in db/db mice	1222:1276	Oral administration of a non-absorbable antibiotic modulated the gut microbiota and improved metabolic endotoxemia and stroke outcomes in db/db mice; these effects were associated with reduction of LPS levels and neuroinflammation in the ischemic brain.
31910709	3	52	theme	experimental	576:587	arg1	stroke					589:594	experimental stroke	576:594	experimental stroke with transient middle cerebral artery occlusion (MCAO)	576:649	We investigated the effects of metabolic endotoxemia after experimental stroke with transient middle cerebral artery occlusion (MCAO) in a murine model of type 2 diabetes (db/db) and phenotypically normal littermates (db/+).
31910709	1	53	theme	potent	180:185	arg1	stimulus					200:207	a potent inflammatory stimulus	178:207	a potent inflammatory stimulus for the innate immune response	178:238	Lipopolysaccharide (LPS) is a major component of the outer membrane of Gram-negative bacteria and a potent inflammatory stimulus for the innate immune response via toll-like receptor (TLR) 4 activation.
31910709	4	54	theme	increased	824:832	arg1	permeability					845:856	increased intestinal permeability	824:856	increased intestinal permeability	824:856	Compared to db/+ mice, db/db mice exhibited an altered gut microbial composition, increased intestinal permeability, and higher plasma LPS levels.
31910709	3	55	theme	endotoxemia	558:568	arg1	effects					537:543	the effects	533:543	the effects of metabolic endotoxemia after experimental stroke with transient middle cerebral artery occlusion (MCAO)	533:649	We investigated the effects of metabolic endotoxemia after experimental stroke with transient middle cerebral artery occlusion (MCAO) in a murine model of type 2 diabetes (db/db) and phenotypically normal littermates (db/+).
31910709	3	56	theme	middle	611:616	arg1	occlusion					634:642	transient middle cerebral artery occlusion	601:642	transient middle cerebral artery occlusion (MCAO)	601:649	We investigated the effects of metabolic endotoxemia after experimental stroke with transient middle cerebral artery occlusion (MCAO) in a murine model of type 2 diabetes (db/db) and phenotypically normal littermates (db/+).
31910709	3	56	theme	middle	611:616	arg1	MCAO					645:648	MCAO	645:648	MCAO	645:648	We investigated the effects of metabolic endotoxemia after experimental stroke with transient middle cerebral artery occlusion (MCAO) in a murine model of type 2 diabetes (db/db) and phenotypically normal littermates (db/+).
31910709	5	57	attach	presented	913:921	arg2	mice					908:911	db/db mice	902:911	db/db mice	902:911	In addition, db/db mice presented increased infarct volumes and higher expression levels of LPS, TLR4, and inflammatory cytokines in the ischemic brain, as well as more severe neurological impairments and reduced survival rates after MCAO.
31910709	5	57	attach	presented	913:921	arg1	addition					892:899	addition	892:899	addition	892:899	In addition, db/db mice presented increased infarct volumes and higher expression levels of LPS, TLR4, and inflammatory cytokines in the ischemic brain, as well as more severe neurological impairments and reduced survival rates after MCAO.
31910709	4	58	theme	plasma	870:875	arg1	levels					881:886	higher plasma LPS levels	863:886	higher plasma LPS levels	863:886	Compared to db/+ mice, db/db mice exhibited an altered gut microbial composition, increased intestinal permeability, and higher plasma LPS levels.
31910709	1	59	dep	TLR	264:266	arg1	activation					271:280	activation	271:280	toll-like receptor (TLR) 4 activation	244:280	Lipopolysaccharide (LPS) is a major component of the outer membrane of Gram-negative bacteria and a potent inflammatory stimulus for the innate immune response via toll-like receptor (TLR) 4 activation.
31910709	6	60	theme	gut	1194:1196	arg1	microbiota					1198:1207	the gut microbiota	1190:1207	the gut microbiota	1190:1207	Oral administration of a non-absorbable antibiotic modulated the gut microbiota and improved metabolic endotoxemia and stroke outcomes in db/db mice; these effects were associated with reduction of LPS levels and neuroinflammation in the ischemic brain.
31910709	5	61	theme	ischemic	1026:1033	arg1	brain					1035:1039	the ischemic brain	1022:1039	the ischemic brain	1022:1039	In addition, db/db mice presented increased infarct volumes and higher expression levels of LPS, TLR4, and inflammatory cytokines in the ischemic brain, as well as more severe neurological impairments and reduced survival rates after MCAO.
31910709	3	62	theme	artery	627:632	arg1	occlusion					634:642	transient middle cerebral artery occlusion	601:642	transient middle cerebral artery occlusion (MCAO)	601:649	We investigated the effects of metabolic endotoxemia after experimental stroke with transient middle cerebral artery occlusion (MCAO) in a murine model of type 2 diabetes (db/db) and phenotypically normal littermates (db/+).
31910709	3	62	theme	artery	627:632	arg1	MCAO					645:648	MCAO	645:648	MCAO	645:648	We investigated the effects of metabolic endotoxemia after experimental stroke with transient middle cerebral artery occlusion (MCAO) in a murine model of type 2 diabetes (db/db) and phenotypically normal littermates (db/+).
31910709	1	63	theme	membrane	139:146	arg1	component					116:124	a major component	108:124	a major component of the outer membrane of Gram-negative bacteria	108:172	Lipopolysaccharide (LPS) is a major component of the outer membrane of Gram-negative bacteria and a potent inflammatory stimulus for the innate immune response via toll-like receptor (TLR) 4 activation.
31910709	1	63	theme	membrane	139:146	arg1	Lipopolysaccharide					80:97	Lipopolysaccharide	80:97	Lipopolysaccharide (LPS)	80:103	Lipopolysaccharide (LPS) is a major component of the outer membrane of Gram-negative bacteria and a potent inflammatory stimulus for the innate immune response via toll-like receptor (TLR) 4 activation.
31910709	1	63	theme	membrane	139:146	arg1	stimulus					200:207	a potent inflammatory stimulus	178:207	a potent inflammatory stimulus for the innate immune response	178:238	Lipopolysaccharide (LPS) is a major component of the outer membrane of Gram-negative bacteria and a potent inflammatory stimulus for the innate immune response via toll-like receptor (TLR) 4 activation.
31910709	1	64	theme	Gram-negative	151:163	arg1	bacteria					165:172	Gram-negative bacteria	151:172	Gram-negative bacteria	151:172	Lipopolysaccharide (LPS) is a major component of the outer membrane of Gram-negative bacteria and a potent inflammatory stimulus for the innate immune response via toll-like receptor (TLR) 4 activation.
31910709	5	65	theme	survival	1102:1109	arg1	rates					1111:1115	reduced survival rates	1094:1115	reduced survival rates	1094:1115	In addition, db/db mice presented increased infarct volumes and higher expression levels of LPS, TLR4, and inflammatory cytokines in the ischemic brain, as well as more severe neurological impairments and reduced survival rates after MCAO.
31910709	6	66	theme	levels	1331:1336	arg1	neuroinflammation					1342:1358	neuroinflammation	1342:1358	neuroinflammation in the ischemic brain	1342:1380	Oral administration of a non-absorbable antibiotic modulated the gut microbiota and improved metabolic endotoxemia and stroke outcomes in db/db mice; these effects were associated with reduction of LPS levels and neuroinflammation in the ischemic brain.
31910709	6	66	theme	levels	1331:1336	arg1	reduction					1314:1322	reduction	1314:1322	reduction of LPS levels	1314:1336	Oral administration of a non-absorbable antibiotic modulated the gut microbiota and improved metabolic endotoxemia and stroke outcomes in db/db mice; these effects were associated with reduction of LPS levels and neuroinflammation in the ischemic brain.
31910709	5	67	theme	higher	953:958	arg1	levels					971:976	higher expression levels	953:976	higher expression levels of LPS, TLR4, and inflammatory cytokines	953:1017	In addition, db/db mice presented increased infarct volumes and higher expression levels of LPS, TLR4, and inflammatory cytokines in the ischemic brain, as well as more severe neurological impairments and reduced survival rates after MCAO.
31910709	4	68	theme	db/+	754:757	arg1	mice					759:762	db/+ mice	754:762	db/+ mice	754:762	Compared to db/+ mice, db/db mice exhibited an altered gut microbial composition, increased intestinal permeability, and higher plasma LPS levels.
31910709	5	69	theme	neurological	1065:1076	arg1	impairments					1078:1088	more severe neurological impairments	1053:1088	more severe neurological impairments	1053:1088	In addition, db/db mice presented increased infarct volumes and higher expression levels of LPS, TLR4, and inflammatory cytokines in the ischemic brain, as well as more severe neurological impairments and reduced survival rates after MCAO.
31910709	2	70	theme	barrier	368:374	arg1	functions					376:384	impaired intestinal barrier functions	348:384	impaired intestinal barrier functions	348:384	Type 2 diabetes is associated with changes in gut microbiota and impaired intestinal barrier functions, leading to translocation of microbiota-derived LPS into the circulatory system, a condition referred to as metabolic endotoxemia.
31910709	5	71	theme	increased	923:931	arg1	volumes					941:947	increased infarct volumes	923:947	increased infarct volumes	923:947	In addition, db/db mice presented increased infarct volumes and higher expression levels of LPS, TLR4, and inflammatory cytokines in the ischemic brain, as well as more severe neurological impairments and reduced survival rates after MCAO.
31910709	2	72	theme	LPS	434:436	arg1	translocation					398:410	translocation	398:410	translocation of microbiota-derived LPS into the circulatory system, a condition referred to as metabolic endotoxemia	398:514	Type 2 diabetes is associated with changes in gut microbiota and impaired intestinal barrier functions, leading to translocation of microbiota-derived LPS into the circulatory system, a condition referred to as metabolic endotoxemia.
31910709	0	73	theme	focal	55:59	arg1	ischemia					70:77	focal cerebral ischemia	55:77	focal cerebral ischemia	55:77	Metabolic endotoxemia promotes neuroinflammation after focal cerebral ischemia.
31910709	6	74	from	reduction	1314:1322	arg1	brain					1376:1380	the ischemic brain	1363:1380	the ischemic brain	1363:1380	Oral administration of a non-absorbable antibiotic modulated the gut microbiota and improved metabolic endotoxemia and stroke outcomes in db/db mice; these effects were associated with reduction of LPS levels and neuroinflammation in the ischemic brain.
31910709	5	75	theme	TLR4	986:989	arg1	rates					1111:1115	reduced survival rates	1094:1115	reduced survival rates	1094:1115	In addition, db/db mice presented increased infarct volumes and higher expression levels of LPS, TLR4, and inflammatory cytokines in the ischemic brain, as well as more severe neurological impairments and reduced survival rates after MCAO.
31910709	5	75	theme	TLR4	986:989	arg1	levels					971:976	higher expression levels	953:976	higher expression levels of LPS, TLR4, and inflammatory cytokines	953:1017	In addition, db/db mice presented increased infarct volumes and higher expression levels of LPS, TLR4, and inflammatory cytokines in the ischemic brain, as well as more severe neurological impairments and reduced survival rates after MCAO.
31910709	5	75	theme	TLR4	986:989	arg1	impairments					1078:1088	more severe neurological impairments	1053:1088	more severe neurological impairments	1053:1088	In addition, db/db mice presented increased infarct volumes and higher expression levels of LPS, TLR4, and inflammatory cytokines in the ischemic brain, as well as more severe neurological impairments and reduced survival rates after MCAO.
31910709	5	75	theme	TLR4	986:989	arg1	volumes					941:947	increased infarct volumes	923:947	increased infarct volumes	923:947	In addition, db/db mice presented increased infarct volumes and higher expression levels of LPS, TLR4, and inflammatory cytokines in the ischemic brain, as well as more severe neurological impairments and reduced survival rates after MCAO.
31910709	5	76	from	impairments	1078:1088	arg1	brain					1035:1039	the ischemic brain	1022:1039	the ischemic brain	1022:1039	In addition, db/db mice presented increased infarct volumes and higher expression levels of LPS, TLR4, and inflammatory cytokines in the ischemic brain, as well as more severe neurological impairments and reduced survival rates after MCAO.
31910709	6	77	from	neuroinflammation	1342:1358	arg1	brain					1376:1380	the ischemic brain	1363:1380	the ischemic brain	1363:1380	Oral administration of a non-absorbable antibiotic modulated the gut microbiota and improved metabolic endotoxemia and stroke outcomes in db/db mice; these effects were associated with reduction of LPS levels and neuroinflammation in the ischemic brain.
31910709	3	78	theme	type	672:675	arg1	db/db					689:693	db/db	689:693	db/db	689:693	We investigated the effects of metabolic endotoxemia after experimental stroke with transient middle cerebral artery occlusion (MCAO) in a murine model of type 2 diabetes (db/db) and phenotypically normal littermates (db/+).
31910709	3	78	theme	type	672:675	arg1	diabetes					679:686	type 2 diabetes	672:686	type 2 diabetes (db/db)	672:694	We investigated the effects of metabolic endotoxemia after experimental stroke with transient middle cerebral artery occlusion (MCAO) in a murine model of type 2 diabetes (db/db) and phenotypically normal littermates (db/+).
31910709	4	79	theme	gut	797:799	arg1	composition					811:821	an altered gut microbial composition	786:821	an altered gut microbial composition	786:821	Compared to db/+ mice, db/db mice exhibited an altered gut microbial composition, increased intestinal permeability, and higher plasma LPS levels.
31910709	2	80	link	microbiota-derived	415:432	arg1	LPS					434:436	microbiota-derived LPS	415:436	microbiota-derived LPS	415:436	Type 2 diabetes is associated with changes in gut microbiota and impaired intestinal barrier functions, leading to translocation of microbiota-derived LPS into the circulatory system, a condition referred to as metabolic endotoxemia.
31910709	6	81	from	outcomes	1255:1262	arg1	mice					1273:1276	db/db mice	1267:1276	db/db mice	1267:1276	Oral administration of a non-absorbable antibiotic modulated the gut microbiota and improved metabolic endotoxemia and stroke outcomes in db/db mice; these effects were associated with reduction of LPS levels and neuroinflammation in the ischemic brain.
31910709	7	82	theme	stroke	1496:1501	arg1	outcomes					1503:1510	stroke outcomes	1496:1510	stroke outcomes	1496:1510	These data suggest that targeting metabolic endotoxemia may be a novel potential therapeutic strategy to improve stroke outcomes.
31910709	5	83	theme	LPS	981:983	arg1	rates					1111:1115	reduced survival rates	1094:1115	reduced survival rates	1094:1115	In addition, db/db mice presented increased infarct volumes and higher expression levels of LPS, TLR4, and inflammatory cytokines in the ischemic brain, as well as more severe neurological impairments and reduced survival rates after MCAO.
31910709	5	83	theme	LPS	981:983	arg1	levels					971:976	higher expression levels	953:976	higher expression levels of LPS, TLR4, and inflammatory cytokines	953:1017	In addition, db/db mice presented increased infarct volumes and higher expression levels of LPS, TLR4, and inflammatory cytokines in the ischemic brain, as well as more severe neurological impairments and reduced survival rates after MCAO.
31910709	5	83	theme	LPS	981:983	arg1	impairments					1078:1088	more severe neurological impairments	1053:1088	more severe neurological impairments	1053:1088	In addition, db/db mice presented increased infarct volumes and higher expression levels of LPS, TLR4, and inflammatory cytokines in the ischemic brain, as well as more severe neurological impairments and reduced survival rates after MCAO.
31910709	5	83	theme	LPS	981:983	arg1	volumes					941:947	increased infarct volumes	923:947	increased infarct volumes	923:947	In addition, db/db mice presented increased infarct volumes and higher expression levels of LPS, TLR4, and inflammatory cytokines in the ischemic brain, as well as more severe neurological impairments and reduced survival rates after MCAO.
31910709	2	84	theme	impaired	348:355	arg1	functions					376:384	impaired intestinal barrier functions	348:384	impaired intestinal barrier functions	348:384	Type 2 diabetes is associated with changes in gut microbiota and impaired intestinal barrier functions, leading to translocation of microbiota-derived LPS into the circulatory system, a condition referred to as metabolic endotoxemia.
31910709	1	85	theme	toll-like	244:252	arg1	TLR					264:266	toll-like receptor (TLR) 4 activation	244:280	toll-like receptor (TLR) 4 activation	244:280	Lipopolysaccharide (LPS) is a major component of the outer membrane of Gram-negative bacteria and a potent inflammatory stimulus for the innate immune response via toll-like receptor (TLR) 4 activation.
32684450	15	0	theme	acetate	2630:2636	arg1	propionate					2638:2647	acetate:propionate	2630:2647	acetate:propionate	2630:2647	Total volatile fatty acid concentration increased linearly, whereas acetate:propionate decreased linearly, as SB replaced WS.
32684450	7	1	theme	matter	1129:1134	arg1	concentration					1301:1313	greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration	1113:1313	greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration	1113:1313	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	8	2	theme	undegraded	1553:1562	arg1	NDF					1564:1566	undegraded NDF	1553:1566	undegraded NDF	1553:1566	Sugarcane bagasse also had greater physically effective NDF (total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves, peNDF8; 63.2 vs. 40.6% of DM) and undegraded NDF after 288 h of incubation (uNDF288; 35.5 vs. 21.2% of DM) contents than WS.
32684450	8	3	theme	Sugarcane	1389:1397	arg1	bagasse					1399:1405	Sugarcane bagasse	1389:1405	Sugarcane bagasse	1389:1405	Sugarcane bagasse also had greater physically effective NDF (total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves, peNDF8; 63.2 vs. 40.6% of DM) and undegraded NDF after 288 h of incubation (uNDF288; 35.5 vs. 21.2% of DM) contents than WS.
32684450	2	4	theme	other	313:317	arg1	sources					332:338	other conventional sources	313:338	other conventional sources of forage	313:348	It is generally less costly compared with other conventional sources of forage.
32684450	7	5	dep	OM	1137:1138	arg1	DM					1159:1160	DM	1159:1160	DM	1159:1160	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	7	5	dep	OM	1137:1138	arg1	%					1154:1154	94.1 vs. 85.1%	1141:1154	%	1154:1154	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	7	6	theme	greater	1113:1119	arg1	concentration					1301:1313	greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration	1113:1313	greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration	1113:1313	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	7	7	theme	DM	1297:1298	arg1	%					1292:1292	19.9 vs. 10.3%	1279:1292	19.9 vs. 10.3% of DM	1279:1298	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	7	7	theme	DM	1297:1298	arg1	DM					1297:1298	DM	1297:1298	DM	1297:1298	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	4	8	theme	replacing	525:533	arg1	WS					535:536	replacing WS	525:536	replacing WS with SB	525:544	This study evaluated the effects of replacing WS with SB in diets offered to mid-lactation dairy cows on milk production and fatty acid profile, intake, digestibility, chewing activity, and ruminal fermentation.
32684450	4	9	from	cows	586:589	arg1	intake					634:639	intake	634:639	intake	634:639	This study evaluated the effects of replacing WS with SB in diets offered to mid-lactation dairy cows on milk production and fatty acid profile, intake, digestibility, chewing activity, and ruminal fermentation.
32684450	4	9	from	cows	586:589	arg1	production					599:608	milk production	594:608	milk production	594:608	This study evaluated the effects of replacing WS with SB in diets offered to mid-lactation dairy cows on milk production and fatty acid profile, intake, digestibility, chewing activity, and ruminal fermentation.
32684450	4	9	from	cows	586:589	arg1	fermentation					687:698	ruminal fermentation	679:698	ruminal fermentation	679:698	This study evaluated the effects of replacing WS with SB in diets offered to mid-lactation dairy cows on milk production and fatty acid profile, intake, digestibility, chewing activity, and ruminal fermentation.
32684450	4	9	from	cows	586:589	arg1	digestibility					642:654	digestibility	642:654	digestibility	642:654	This study evaluated the effects of replacing WS with SB in diets offered to mid-lactation dairy cows on milk production and fatty acid profile, intake, digestibility, chewing activity, and ruminal fermentation.
32684450	4	9	from	cows	586:589	arg1	activity					665:672	chewing activity	657:672	chewing activity	657:672	This study evaluated the effects of replacing WS with SB in diets offered to mid-lactation dairy cows on milk production and fatty acid profile, intake, digestibility, chewing activity, and ruminal fermentation.
32684450	4	9	from	cows	586:589	arg1	profile					625:631	fatty acid profile	614:631	fatty acid profile	614:631	This study evaluated the effects of replacing WS with SB in diets offered to mid-lactation dairy cows on milk production and fatty acid profile, intake, digestibility, chewing activity, and ruminal fermentation.
32684450	1	10	theme	Sugarcane	158:166	arg1	bagasse					168:174	Sugarcane bagasse	158:174	Sugarcane bagasse (SB)	158:179	Sugarcane bagasse (SB) is a low-quality roughage source that is often plentiful during times of forage shortage.
32684450	1	10	theme	Sugarcane	158:166	arg1	source					207:212	a low-quality roughage source	184:212	a low-quality roughage source that is often plentiful during times of forage shortage	184:268	Sugarcane bagasse (SB) is a low-quality roughage source that is often plentiful during times of forage shortage.
32684450	1	10	theme	Sugarcane	158:166	arg1	plentiful					228:236	plentiful	228:236	plentiful	228:236	Sugarcane bagasse (SB) is a low-quality roughage source that is often plentiful during times of forage shortage.
32684450	1	10	theme	Sugarcane	158:166	arg1	SB					177:178	SB	177:178	SB	177:178	Sugarcane bagasse (SB) is a low-quality roughage source that is often plentiful during times of forage shortage.
32684450	8	11	dep	greater	1416:1422	arg1	NDF					1564:1566	undegraded NDF	1553:1566	undegraded NDF	1553:1566	Sugarcane bagasse also had greater physically effective NDF (total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves, peNDF8; 63.2 vs. 40.6% of DM) and undegraded NDF after 288 h of incubation (uNDF288; 35.5 vs. 21.2% of DM) contents than WS.
32684450	8	11	dep	greater	1416:1422	arg1	NDF					1445:1447	physically effective NDF	1424:1447	physically effective NDF (total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves, peNDF8; 63.2 vs. 40.6% of DM)	1424:1547	Sugarcane bagasse also had greater physically effective NDF (total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves, peNDF8; 63.2 vs. 40.6% of DM) and undegraded NDF after 288 h of incubation (uNDF288; 35.5 vs. 21.2% of DM) contents than WS.
32684450	8	12	theme	incubation	1583:1592	arg1	contents					1626:1633	incubation (uNDF288; 35.5 vs. 21.2% of DM) contents	1583:1633	incubation (uNDF288; 35.5 vs. 21.2% of DM) contents	1583:1633	Sugarcane bagasse also had greater physically effective NDF (total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves, peNDF8; 63.2 vs. 40.6% of DM) and undegraded NDF after 288 h of incubation (uNDF288; 35.5 vs. 21.2% of DM) contents than WS.
32684450	4	13	theme	milk	594:597	arg1	production					599:608	milk production	594:608	milk production	594:608	This study evaluated the effects of replacing WS with SB in diets offered to mid-lactation dairy cows on milk production and fatty acid profile, intake, digestibility, chewing activity, and ruminal fermentation.
32684450	6	14	theme	%	994:994	arg1	WS					996:997	27% WS	992:997	27% WS	992:997	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	13	15	theme	true	2375:2378	arg1	protein					2380:2386	true protein	2375:2386	true protein	2375:2386	Milk yield (37.0 kg/d), energy-corrected milk yield (ECM; 38.2 kg/d), feed efficiency (1.44 kg ECM yield/kg DM intake), and milk composition (fat, 3.89%; true protein, 2.90%) did not differ among diets.
32684450	16	16	theme	low-forage	2764:2773	arg1	diets					2775:2779	low-forage diets	2764:2779	low-forage diets with similar uNDF30 concentrations	2764:2814	The results indicate that replacement of WS with increasing levels of SB in low-forage diets with similar uNDF30 concentrations did not affect performance of mid-lactation dairy cows.
32684450	6	17	theme	18	1035:1036	arg1	%					1037:1037	%	1037:1037	%	1037:1037	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	9	18	theme	content	1697:1703	arg1	NDF					1659:1661	The undegraded NDF	1644:1661	The undegraded NDF after 30 h of incubation (uNDF30) content	1644:1703	The undegraded NDF after 30 h of incubation (uNDF30) content was similar for all diets; however, peNDF8 concentration and proportion of long particles (retained on a 19-mm sieve) increased linearly as SB inclusion in the diets increased.
32684450	9	18	theme	content	1697:1703	arg1	similar					1709:1715	similar	1709:1715	similar	1709:1715	The undegraded NDF after 30 h of incubation (uNDF30) content was similar for all diets; however, peNDF8 concentration and proportion of long particles (retained on a 19-mm sieve) increased linearly as SB inclusion in the diets increased.
32684450	5	19	theme	standard	750:757	arg1	deviation					759:767	mean ± standard deviation	743:767	mean ± standard deviation	743:767	Nine multiparous Holstein cows averaging (mean ± standard deviation) 105 ± 12 d in milk, 42.1 ± 2.9 kg of milk/d, and 617 ± 59 kg of body weight were used in a replicated 3 × 3 Latin square with 21-d periods.
32684450	5	19	theme	standard	750:757	arg1	105					770:772	105	770:772	105	770:772	Nine multiparous Holstein cows averaging (mean ± standard deviation) 105 ± 12 d in milk, 42.1 ± 2.9 kg of milk/d, and 617 ± 59 kg of body weight were used in a replicated 3 × 3 Latin square with 21-d periods.
32684450	17	20	from	decrease	2980:2987	arg1	digestibility					3004:3016	total-tract digestibility	2992:3016	total-tract digestibility of diets	2992:3025	We conclude that SB can be used as a fiber source in diets fed to dairy cows in mid-lactation; however, the decrease in total-tract digestibility of diets may decrease lactational performance when fed to high-producing dairy cows.
32684450	6	21	theme	27	992:993	arg1	%					994:994	%	994:994	%	994:994	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	8	22	theme	19-mm	1505:1509	arg1	sieves					1511:1516	the 8-mm + 19-mm sieves	1494:1516	the 8-mm + 19-mm sieves	1494:1516	Sugarcane bagasse also had greater physically effective NDF (total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves, peNDF8; 63.2 vs. 40.6% of DM) and undegraded NDF after 288 h of incubation (uNDF288; 35.5 vs. 21.2% of DM) contents than WS.
32684450	6	23	dep	0SB	961:963	arg1	1					958:958	1	958:958	1	958:958	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	17	24	theme	lactational	3040:3050	arg1	performance					3052:3062	lactational performance	3040:3062	lactational performance	3040:3062	We conclude that SB can be used as a fiber source in diets fed to dairy cows in mid-lactation; however, the decrease in total-tract digestibility of diets may decrease lactational performance when fed to high-producing dairy cows.
32684450	13	25	theme	energy-corrected	2245:2260	arg1	yield					2267:2271	energy-corrected milk yield	2245:2271	energy-corrected milk yield (ECM; 38.2 kg/d)	2245:2288	Milk yield (37.0 kg/d), energy-corrected milk yield (ECM; 38.2 kg/d), feed efficiency (1.44 kg ECM yield/kg DM intake), and milk composition (fat, 3.89%; true protein, 2.90%) did not differ among diets.
32684450	15	26	dep	increased	2602:2610	arg1	whereas					2622:2628	whereas	2622:2628	whereas	2622:2628	Total volatile fatty acid concentration increased linearly, whereas acetate:propionate decreased linearly, as SB replaced WS.
32684450	7	27	dep	ADF	1239:1241	arg1	%					1257:1257	62.9 vs. 45.2%	1244:1257	%	1257:1257	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	7	27	dep	ADF	1239:1241	arg1	DM					1262:1263	DM	1262:1263	DM	1262:1263	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	6	28	theme	9	1025:1025	arg1	%					1026:1026	%	1026:1026	%	1026:1026	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	4	29	with	WS	535:536	arg1	SB					543:544	SB	543:544	SB	543:544	This study evaluated the effects of replacing WS with SB in diets offered to mid-lactation dairy cows on milk production and fatty acid profile, intake, digestibility, chewing activity, and ruminal fermentation.
32684450	6	30	theme	0	982:982	arg1	%					983:983	%	983:983	%	983:983	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	0	31	theme	chewing	140:146	arg1	cows					98:101	mid-lactation dairy cows	78:101	mid-lactation dairy cows: Milk production, digestibility, and chewing behavior	78:155	Substitution of wheat straw with sugarcane bagasse in low-forage diets fed to mid-lactation dairy cows: Milk production, digestibility, and chewing behavior.
32684450	0	31	theme	chewing	140:146	arg1	behavior					148:155	chewing behavior	140:155	chewing behavior	140:155	Substitution of wheat straw with sugarcane bagasse in low-forage diets fed to mid-lactation dairy cows: Milk production, digestibility, and chewing behavior.
32684450	5	32	theme	Latin	878:882	arg1	square					884:889	Latin square	878:889	a replicated 3 × 3 Latin square with 21-d periods	859:907	Nine multiparous Holstein cows averaging (mean ± standard deviation) 105 ± 12 d in milk, 42.1 ± 2.9 kg of milk/d, and 617 ± 59 kg of body weight were used in a replicated 3 × 3 Latin square with 21-d periods.
32684450	9	33	theme	long	1780:1783	arg1	particles					1785:1793	long particles	1780:1793	long particles	1780:1793	The undegraded NDF after 30 h of incubation (uNDF30) content was similar for all diets; however, peNDF8 concentration and proportion of long particles (retained on a 19-mm sieve) increased linearly as SB inclusion in the diets increased.
32684450	15	34	theme	Total	2562:2566	arg1	concentration					2588:2600	Total volatile fatty acid concentration	2562:2600	Total volatile fatty acid concentration	2562:2600	Total volatile fatty acid concentration increased linearly, whereas acetate:propionate decreased linearly, as SB replaced WS.
32684450	7	35	theme	DM	1262:1263	arg1	%					1257:1257	62.9 vs. 45.2%	1244:1257	%	1257:1257	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	7	35	theme	DM	1262:1263	arg1	DM					1262:1263	DM	1262:1263	DM	1262:1263	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	14	36	dep	pH	2512:2513	arg1	ruminocentesis					2516:2529	ruminocentesis	2516:2529	ruminocentesis	2516:2529	Increasing SB concentration of the diet linearly increased rumination time, but ruminal pH (ruminocentesis, 4 h after feeding) decreased.
32684450	14	36	dep	pH	2512:2513	arg1	h					2534:2534	4 h	2532:2534	4 h after feeding	2532:2548	Increasing SB concentration of the diet linearly increased rumination time, but ruminal pH (ruminocentesis, 4 h after feeding) decreased.
32684450	14	37	theme	diet	2459:2462	arg1	concentration					2438:2450	Increasing SB concentration	2424:2450	Increasing SB concentration of the diet	2424:2462	Increasing SB concentration of the diet linearly increased rumination time, but ruminal pH (ruminocentesis, 4 h after feeding) decreased.
32684450	13	38	dep	fat	2363:2365	arg1	protein					2380:2386	true protein	2375:2386	true protein	2375:2386	Milk yield (37.0 kg/d), energy-corrected milk yield (ECM; 38.2 kg/d), feed efficiency (1.44 kg ECM yield/kg DM intake), and milk composition (fat, 3.89%; true protein, 2.90%) did not differ among diets.
32684450	0	39	theme	low-forage	54:63	arg1	diets					65:69	low-forage diets	54:69	low-forage diets	54:69	Substitution of wheat straw with sugarcane bagasse in low-forage diets fed to mid-lactation dairy cows: Milk production, digestibility, and chewing behavior.
32684450	5	40	theme	mean	743:746	arg1	deviation					759:767	mean ± standard deviation	743:767	mean ± standard deviation	743:767	Nine multiparous Holstein cows averaging (mean ± standard deviation) 105 ± 12 d in milk, 42.1 ± 2.9 kg of milk/d, and 617 ± 59 kg of body weight were used in a replicated 3 × 3 Latin square with 21-d periods.
32684450	5	40	theme	mean	743:746	arg1	105					770:772	105	770:772	105	770:772	Nine multiparous Holstein cows averaging (mean ± standard deviation) 105 ± 12 d in milk, 42.1 ± 2.9 kg of milk/d, and 617 ± 59 kg of body weight were used in a replicated 3 × 3 Latin square with 21-d periods.
32684450	7	41	contain	had	1109:1111	arg1	bagasse					1101:1107	Sugarcane bagasse	1091:1107	Sugarcane bagasse	1091:1107	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	7	41	contain	had	1109:1111	arg2	concentration					1366:1378	less crude protein (CP; 2.63 vs. 3.72% of DM) concentration	1320:1378	less crude protein (CP; 2.63 vs. 3.72% of DM) concentration	1320:1378	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	7	41	contain	had	1109:1111	arg2	concentration					1301:1313	greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration	1113:1313	greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration	1113:1313	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	7	42	theme	fiber	1182:1186	arg1	concentration					1301:1313	greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration	1113:1313	greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration	1113:1313	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	1	43	dep	shortage	261:268	arg1	forage					254:259	forage	254:259	forage	254:259	Sugarcane bagasse (SB) is a low-quality roughage source that is often plentiful during times of forage shortage.
32684450	1	43	dep	shortage	261:268	arg1	times					245:249	times	245:249	times	245:249	Sugarcane bagasse (SB) is a low-quality roughage source that is often plentiful during times of forage shortage.
32684450	7	44	dep	fiber	1232:1236	arg1	ADF					1239:1241	ADF	1239:1241	ADF; 62.9 vs. 45.2% of DM	1239:1263	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	4	45	theme	acid	620:623	arg1	profile					625:631	fatty acid profile	614:631	fatty acid profile	614:631	This study evaluated the effects of replacing WS with SB in diets offered to mid-lactation dairy cows on milk production and fatty acid profile, intake, digestibility, chewing activity, and ruminal fermentation.
32684450	9	46	theme	SB	1845:1846	arg1	inclusion					1848:1856	SB inclusion	1845:1856	SB inclusion in the diets	1845:1869	The undegraded NDF after 30 h of incubation (uNDF30) content was similar for all diets; however, peNDF8 concentration and proportion of long particles (retained on a 19-mm sieve) increased linearly as SB inclusion in the diets increased.
32684450	16	47	theme	dairy	2860:2864	arg1	cows					2866:2869	mid-lactation dairy cows	2846:2869	mid-lactation dairy cows	2846:2869	The results indicate that replacement of WS with increasing levels of SB in low-forage diets with similar uNDF30 concentrations did not affect performance of mid-lactation dairy cows.
32684450	7	48	theme	neutral	1164:1170	arg1	fiber					1182:1186	neutral detergent fiber	1164:1186	neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM)	1164:1214	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	7	49	dep	CP	1340:1341	arg1	%					1357:1357	2.63 vs. 3.72%	1344:1357	CP; 2.63 vs. 3.72% of DM	1340:1363	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	7	49	dep	CP	1340:1341	arg1	DM					1362:1363	DM	1362:1363	DM	1362:1363	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	14	50	theme	SB	2435:2436	arg1	concentration					2438:2450	Increasing SB concentration	2424:2450	Increasing SB concentration of the diet	2424:2462	Increasing SB concentration of the diet linearly increased rumination time, but ruminal pH (ruminocentesis, 4 h after feeding) decreased.
32684450	11	51	theme	uNDF288	2085:2091	arg1	intakes					2093:2099	ADF and uNDF288 intakes	2077:2099	ADF and uNDF288 intakes	2077:2099	Intakes of DM (26.53 kg/d) and NDF (8.58 kg/d) did not differ among the treatments, but intakes of OM and CP decreased, whereas ADF and uNDF288 intakes increased with SB inclusion level.
32684450	10	52	theme	long	1915:1918	arg1	particles					1920:1928	long particles	1915:1928	long particles	1915:1928	Cows increasingly sorted against long particles as SB replaced WS.
32684450	0	53	theme	mid-lactation	78:90	arg1	digestibility					121:133	digestibility	121:133	digestibility	121:133	Substitution of wheat straw with sugarcane bagasse in low-forage diets fed to mid-lactation dairy cows: Milk production, digestibility, and chewing behavior.
32684450	0	53	theme	mid-lactation	78:90	arg1	cows					98:101	mid-lactation dairy cows	78:101	mid-lactation dairy cows: Milk production, digestibility, and chewing behavior	78:155	Substitution of wheat straw with sugarcane bagasse in low-forage diets fed to mid-lactation dairy cows: Milk production, digestibility, and chewing behavior.
32684450	0	53	theme	mid-lactation	78:90	arg1	production					109:118	Milk production	104:118	Milk production	104:118	Substitution of wheat straw with sugarcane bagasse in low-forage diets fed to mid-lactation dairy cows: Milk production, digestibility, and chewing behavior.
32684450	0	53	theme	mid-lactation	78:90	arg1	behavior					148:155	chewing behavior	140:155	chewing behavior	140:155	Substitution of wheat straw with sugarcane bagasse in low-forage diets fed to mid-lactation dairy cows: Milk production, digestibility, and chewing behavior.
32684450	17	54	theme	total-tract	2992:3002	arg1	digestibility					3004:3016	total-tract digestibility	2992:3016	total-tract digestibility of diets	2992:3025	We conclude that SB can be used as a fiber source in diets fed to dairy cows in mid-lactation; however, the decrease in total-tract digestibility of diets may decrease lactational performance when fed to high-producing dairy cows.
32684450	9	55	theme	19-mm	1810:1814	arg1	sieve					1816:1820	a 19-mm sieve	1808:1820	a 19-mm sieve	1808:1820	The undegraded NDF after 30 h of incubation (uNDF30) content was similar for all diets; however, peNDF8 concentration and proportion of long particles (retained on a 19-mm sieve) increased linearly as SB inclusion in the diets increased.
32684450	0	56	dep	cows	98:101	arg1	digestibility					121:133	digestibility	121:133	digestibility	121:133	Substitution of wheat straw with sugarcane bagasse in low-forage diets fed to mid-lactation dairy cows: Milk production, digestibility, and chewing behavior.
32684450	0	56	dep	cows	98:101	arg1	cows					98:101	mid-lactation dairy cows	78:101	mid-lactation dairy cows: Milk production, digestibility, and chewing behavior	78:155	Substitution of wheat straw with sugarcane bagasse in low-forage diets fed to mid-lactation dairy cows: Milk production, digestibility, and chewing behavior.
32684450	0	56	dep	cows	98:101	arg1	production					109:118	Milk production	104:118	Milk production	104:118	Substitution of wheat straw with sugarcane bagasse in low-forage diets fed to mid-lactation dairy cows: Milk production, digestibility, and chewing behavior.
32684450	0	56	dep	cows	98:101	arg1	behavior					148:155	chewing behavior	140:155	chewing behavior	140:155	Substitution of wheat straw with sugarcane bagasse in low-forage diets fed to mid-lactation dairy cows: Milk production, digestibility, and chewing behavior.
32684450	15	57	theme	acid	2583:2586	arg1	concentration					2588:2600	Total volatile fatty acid concentration	2562:2600	Total volatile fatty acid concentration	2562:2600	Total volatile fatty acid concentration increased linearly, whereas acetate:propionate decreased linearly, as SB replaced WS.
32684450	0	58	from	bagasse	43:49	arg1	diets					65:69	low-forage diets	54:69	low-forage diets	54:69	Substitution of wheat straw with sugarcane bagasse in low-forage diets fed to mid-lactation dairy cows: Milk production, digestibility, and chewing behavior.
32684450	9	59	theme	undegraded	1648:1657	arg1	NDF					1659:1661	The undegraded NDF	1644:1661	The undegraded NDF after 30 h of incubation (uNDF30) content	1644:1703	The undegraded NDF after 30 h of incubation (uNDF30) content was similar for all diets; however, peNDF8 concentration and proportion of long particles (retained on a 19-mm sieve) increased linearly as SB inclusion in the diets increased.
32684450	9	59	theme	undegraded	1648:1657	arg1	similar					1709:1715	similar	1709:1715	similar	1709:1715	The undegraded NDF after 30 h of incubation (uNDF30) content was similar for all diets; however, peNDF8 concentration and proportion of long particles (retained on a 19-mm sieve) increased linearly as SB inclusion in the diets increased.
32684450	11	60	theme	ADF	2077:2079	arg1	intakes					2093:2099	ADF and uNDF288 intakes	2077:2099	ADF and uNDF288 intakes	2077:2099	Intakes of DM (26.53 kg/d) and NDF (8.58 kg/d) did not differ among the treatments, but intakes of OM and CP decreased, whereas ADF and uNDF288 intakes increased with SB inclusion level.
32684450	8	61	dep	uNDF288	1595:1601	arg1	DM					1622:1623	DM	1622:1623	DM	1622:1623	Sugarcane bagasse also had greater physically effective NDF (total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves, peNDF8; 63.2 vs. 40.6% of DM) and undegraded NDF after 288 h of incubation (uNDF288; 35.5 vs. 21.2% of DM) contents than WS.
32684450	8	61	dep	uNDF288	1595:1601	arg1	%					1617:1617	35.5 vs. 21.2%	1604:1617	uNDF288; 35.5 vs. 21.2% of DM	1595:1623	Sugarcane bagasse also had greater physically effective NDF (total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves, peNDF8; 63.2 vs. 40.6% of DM) and undegraded NDF after 288 h of incubation (uNDF288; 35.5 vs. 21.2% of DM) contents than WS.
32684450	8	62	theme	total	1450:1454	arg1	peNDF8					1519:1524	peNDF8	1519:1524	peNDF8	1519:1524	Sugarcane bagasse also had greater physically effective NDF (total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves, peNDF8; 63.2 vs. 40.6% of DM) and undegraded NDF after 288 h of incubation (uNDF288; 35.5 vs. 21.2% of DM) contents than WS.
32684450	8	62	theme	total	1450:1454	arg1	NDF					1464:1466	total dietary NDF	1450:1466	total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves	1450:1516	Sugarcane bagasse also had greater physically effective NDF (total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves, peNDF8; 63.2 vs. 40.6% of DM) and undegraded NDF after 288 h of incubation (uNDF288; 35.5 vs. 21.2% of DM) contents than WS.
32684450	0	63	theme	sugarcane	33:41	arg1	bagasse					43:49	sugarcane bagasse	33:49	sugarcane bagasse in low-forage diets	33:69	Substitution of wheat straw with sugarcane bagasse in low-forage diets fed to mid-lactation dairy cows: Milk production, digestibility, and chewing behavior.
32684450	15	64	theme	volatile	2568:2575	arg1	concentration					2588:2600	Total volatile fatty acid concentration	2562:2600	Total volatile fatty acid concentration	2562:2600	Total volatile fatty acid concentration increased linearly, whereas acetate:propionate decreased linearly, as SB replaced WS.
32684450	8	65	theme	8-mm	1498:1501	arg1	sieves					1511:1516	the 8-mm + 19-mm sieves	1494:1516	the 8-mm + 19-mm sieves	1494:1516	Sugarcane bagasse also had greater physically effective NDF (total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves, peNDF8; 63.2 vs. 40.6% of DM) and undegraded NDF after 288 h of incubation (uNDF288; 35.5 vs. 21.2% of DM) contents than WS.
32684450	11	66	theme	SB	2116:2117	arg1	level					2129:2133	SB inclusion level	2116:2133	SB inclusion level	2116:2133	Intakes of DM (26.53 kg/d) and NDF (8.58 kg/d) did not differ among the treatments, but intakes of OM and CP decreased, whereas ADF and uNDF288 intakes increased with SB inclusion level.
32684450	13	67	dep	yield	2267:2271	arg1	ECM					2274:2276	ECM	2274:2276	ECM; 38.2 kg/d	2274:2287	Milk yield (37.0 kg/d), energy-corrected milk yield (ECM; 38.2 kg/d), feed efficiency (1.44 kg ECM yield/kg DM intake), and milk composition (fat, 3.89%; true protein, 2.90%) did not differ among diets.
32684450	8	68	dep	incubation	1583:1592	arg1	uNDF288					1595:1601	uNDF288	1595:1601	uNDF288; 35.5 vs. 21.2% of DM	1595:1623	Sugarcane bagasse also had greater physically effective NDF (total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves, peNDF8; 63.2 vs. 40.6% of DM) and undegraded NDF after 288 h of incubation (uNDF288; 35.5 vs. 21.2% of DM) contents than WS.
32684450	6	69	theme	9	1084:1084	arg1	%					1085:1085	%	1085:1085	%	1085:1085	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	6	70	dep	18SB	1051:1054	arg1	3					1048:1048	3	1048:1048	3	1048:1048	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	7	71	theme	detergent	1222:1230	arg1	fiber					1232:1236	acid detergent fiber	1217:1236	acid detergent fiber (ADF; 62.9 vs. 45.2% of DM)	1217:1264	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	8	72	theme	contents	1626:1633	arg1	h					1578:1578	288 h	1574:1578	288 h of incubation (uNDF288; 35.5 vs. 21.2% of DM) contents	1574:1633	Sugarcane bagasse also had greater physically effective NDF (total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves, peNDF8; 63.2 vs. 40.6% of DM) and undegraded NDF after 288 h of incubation (uNDF288; 35.5 vs. 21.2% of DM) contents than WS.
32684450	16	73	theme	similar	2786:2792	arg1	concentrations					2801:2814	similar uNDF30 concentrations	2786:2814	similar uNDF30 concentrations	2786:2814	The results indicate that replacement of WS with increasing levels of SB in low-forage diets with similar uNDF30 concentrations did not affect performance of mid-lactation dairy cows.
32684450	11	74	theme	OM	2048:2049	arg1	intakes					2037:2043	intakes	2037:2043	intakes of OM and CP	2037:2056	Intakes of DM (26.53 kg/d) and NDF (8.58 kg/d) did not differ among the treatments, but intakes of OM and CP decreased, whereas ADF and uNDF288 intakes increased with SB inclusion level.
32684450	7	75	dep	fiber	1182:1186	arg1	NDF					1189:1191	NDF	1189:1191	NDF; 86.2 vs. 76.4% of DM	1189:1213	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	0	76	theme	wheat	16:20	arg1	straw					22:26	wheat straw	16:26	wheat straw	16:26	Substitution of wheat straw with sugarcane bagasse in low-forage diets fed to mid-lactation dairy cows: Milk production, digestibility, and chewing behavior.
32684450	7	77	theme	DM	1362:1363	arg1	%					1357:1357	2.63 vs. 3.72%	1344:1357	CP; 2.63 vs. 3.72% of DM	1340:1363	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	7	77	theme	DM	1362:1363	arg1	DM					1362:1363	DM	1362:1363	DM	1362:1363	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	13	78	theme	yield/kg	2320:2327	arg1	intake					2332:2337	1.44 kg ECM yield/kg DM intake	2308:2337	1.44 kg ECM yield/kg DM intake	2308:2337	Milk yield (37.0 kg/d), energy-corrected milk yield (ECM; 38.2 kg/d), feed efficiency (1.44 kg ECM yield/kg DM intake), and milk composition (fat, 3.89%; true protein, 2.90%) did not differ among diets.
32684450	13	78	theme	yield/kg	2320:2327	arg1	efficiency					2296:2305	feed efficiency	2291:2305	feed efficiency (1.44 kg ECM yield/kg DM intake)	2291:2338	Milk yield (37.0 kg/d), energy-corrected milk yield (ECM; 38.2 kg/d), feed efficiency (1.44 kg ECM yield/kg DM intake), and milk composition (fat, 3.89%; true protein, 2.90%) did not differ among diets.
32684450	6	79	dep	were	921:924	arg1	matter					944:949	dietary dry matter	932:949	dietary dry matter	932:949	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	6	79	dep	were	921:924	arg1	diet					1009:1012	diet	1009:1012	diet containing 9% SB and 18% WS	1009:1040	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	6	79	dep	were	921:924	arg1	18SB					1051:1054	(3) 18SB	1047:1054	(3) 18SB	1047:1054	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	6	79	dep	were	921:924	arg1	0SB					961:963	(1) 0SB	957:963	(1) 0SB	957:963	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	6	79	dep	were	921:924	arg1	%					927:927	%	927:927	% of dietary dry matter	927:949	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	6	79	dep	were	921:924	arg1	DM					952:953	DM	952:953	DM	952:953	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	6	79	dep	were	921:924	arg1	diet					1057:1060	diet	1057:1060	diet	1057:1060	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	6	79	dep	were	921:924	arg1	9SB					1004:1006	(2) 9SB	1000:1006	(2) 9SB	1000:1006	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	6	79	dep	were	921:924	arg1	diet					966:969	diet	966:969	diet containing 0% SB and 27% WS	966:997	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	13	80	dep	composition	2350:2360	arg1	fat					2363:2365	fat	2363:2365	fat	2363:2365	Milk yield (37.0 kg/d), energy-corrected milk yield (ECM; 38.2 kg/d), feed efficiency (1.44 kg ECM yield/kg DM intake), and milk composition (fat, 3.89%; true protein, 2.90%) did not differ among diets.
32684450	13	80	dep	composition	2350:2360	arg1	%					2372:2372	3.89%	2368:2372	3.89%	2368:2372	Milk yield (37.0 kg/d), energy-corrected milk yield (ECM; 38.2 kg/d), feed efficiency (1.44 kg ECM yield/kg DM intake), and milk composition (fat, 3.89%; true protein, 2.90%) did not differ among diets.
32684450	13	80	dep	composition	2350:2360	arg1	%					2393:2393	2.90%	2389:2393	2.90%	2389:2393	Milk yield (37.0 kg/d), energy-corrected milk yield (ECM; 38.2 kg/d), feed efficiency (1.44 kg ECM yield/kg DM intake), and milk composition (fat, 3.89%; true protein, 2.90%) did not differ among diets.
32684450	6	81	theme	18	1073:1074	arg1	%					1075:1075	%	1075:1075	%	1075:1075	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	7	82	theme	protein	1331:1337	arg1	concentration					1366:1378	less crude protein (CP; 2.63 vs. 3.72% of DM) concentration	1320:1378	less crude protein (CP; 2.63 vs. 3.72% of DM) concentration	1320:1378	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	5	83	used	used	851:854	arg2	cows					727:730	Nine multiparous Holstein cows	701:730	Nine multiparous Holstein cows averaging (mean ± standard deviation) 105 ± 12 d in milk, 42.1 ± 2.9 kg of milk/d, and 617 ± 59 kg of body weight	701:844	Nine multiparous Holstein cows averaging (mean ± standard deviation) 105 ± 12 d in milk, 42.1 ± 2.9 kg of milk/d, and 617 ± 59 kg of body weight were used in a replicated 3 × 3 Latin square with 21-d periods.
32684450	5	84	theme	Holstein	718:725	arg1	cows					727:730	Nine multiparous Holstein cows	701:730	Nine multiparous Holstein cows averaging (mean ± standard deviation) 105 ± 12 d in milk, 42.1 ± 2.9 kg of milk/d, and 617 ± 59 kg of body weight	701:844	Nine multiparous Holstein cows averaging (mean ± standard deviation) 105 ± 12 d in milk, 42.1 ± 2.9 kg of milk/d, and 617 ± 59 kg of body weight were used in a replicated 3 × 3 Latin square with 21-d periods.
32684450	6	85	theme	dry	940:942	arg1	matter					944:949	dietary dry matter	932:949	dietary dry matter	932:949	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	11	86	theme	DM	1960:1961	arg1	Intakes					1949:1955	Intakes	1949:1955	Intakes of DM (26.53 kg/d) and NDF (8.58 kg/d)	1949:1994	Intakes of DM (26.53 kg/d) and NDF (8.58 kg/d) did not differ among the treatments, but intakes of OM and CP decreased, whereas ADF and uNDF288 intakes increased with SB inclusion level.
32684450	16	87	theme	WS	2729:2730	arg1	replacement					2714:2724	replacement	2714:2724	replacement of WS with increasing levels of SB in low-forage diets with similar uNDF30 concentrations	2714:2814	The results indicate that replacement of WS with increasing levels of SB in low-forage diets with similar uNDF30 concentrations did not affect performance of mid-lactation dairy cows.
32684450	0	88	with	Substitution	0:11	arg1	bagasse					43:49	sugarcane bagasse	33:49	sugarcane bagasse in low-forage diets	33:69	Substitution of wheat straw with sugarcane bagasse in low-forage diets fed to mid-lactation dairy cows: Milk production, digestibility, and chewing behavior.
32684450	12	89	theme	CP	2171:2172	arg1	digestibilities					2148:2162	Total-tract digestibilities	2136:2162	Total-tract digestibilities of OM, CP, and NDF	2136:2181	Total-tract digestibilities of OM, CP, and NDF decreased linearly as SB replaced WS.
32684450	1	90	theme	low-quality	186:196	arg1	source					207:212	a low-quality roughage source	184:212	a low-quality roughage source that is often plentiful during times of forage shortage	184:268	Sugarcane bagasse (SB) is a low-quality roughage source that is often plentiful during times of forage shortage.
32684450	1	90	theme	low-quality	186:196	arg1	bagasse					168:174	Sugarcane bagasse	158:174	Sugarcane bagasse (SB)	158:179	Sugarcane bagasse (SB) is a low-quality roughage source that is often plentiful during times of forage shortage.
32684450	1	90	theme	low-quality	186:196	arg1	plentiful					228:236	plentiful	228:236	plentiful	228:236	Sugarcane bagasse (SB) is a low-quality roughage source that is often plentiful during times of forage shortage.
32684450	5	91	theme	weight	839:844	arg1	kg					828:829	kg	828:829	kg	828:829	Nine multiparous Holstein cows averaging (mean ± standard deviation) 105 ± 12 d in milk, 42.1 ± 2.9 kg of milk/d, and 617 ± 59 kg of body weight were used in a replicated 3 × 3 Latin square with 21-d periods.
32684450	5	91	theme	weight	839:844	arg1	weight					839:844	body weight	834:844	body weight	834:844	Nine multiparous Holstein cows averaging (mean ± standard deviation) 105 ± 12 d in milk, 42.1 ± 2.9 kg of milk/d, and 617 ± 59 kg of body weight were used in a replicated 3 × 3 Latin square with 21-d periods.
32684450	13	92	theme	kg	2313:2314	arg1	intake					2332:2337	1.44 kg ECM yield/kg DM intake	2308:2337	1.44 kg ECM yield/kg DM intake	2308:2337	Milk yield (37.0 kg/d), energy-corrected milk yield (ECM; 38.2 kg/d), feed efficiency (1.44 kg ECM yield/kg DM intake), and milk composition (fat, 3.89%; true protein, 2.90%) did not differ among diets.
32684450	13	92	theme	kg	2313:2314	arg1	efficiency					2296:2305	feed efficiency	2291:2305	feed efficiency (1.44 kg ECM yield/kg DM intake)	2291:2338	Milk yield (37.0 kg/d), energy-corrected milk yield (ECM; 38.2 kg/d), feed efficiency (1.44 kg ECM yield/kg DM intake), and milk composition (fat, 3.89%; true protein, 2.90%) did not differ among diets.
32684450	4	93	theme	WS	535:536	arg1	effects					514:520	the effects	510:520	the effects of replacing WS with SB in diets offered to mid-lactation dairy cows on milk production and fatty acid profile, intake, digestibility, chewing activity, and ruminal fermentation	510:698	This study evaluated the effects of replacing WS with SB in diets offered to mid-lactation dairy cows on milk production and fatty acid profile, intake, digestibility, chewing activity, and ruminal fermentation.
32684450	12	94	theme	NDF	2179:2181	arg1	digestibilities					2148:2162	Total-tract digestibilities	2136:2162	Total-tract digestibilities of OM, CP, and NDF	2136:2181	Total-tract digestibilities of OM, CP, and NDF decreased linearly as SB replaced WS.
32684450	11	95	theme	CP	2055:2056	arg1	intakes					2037:2043	intakes	2037:2043	intakes of OM and CP	2037:2056	Intakes of DM (26.53 kg/d) and NDF (8.58 kg/d) did not differ among the treatments, but intakes of OM and CP decreased, whereas ADF and uNDF288 intakes increased with SB inclusion level.
32684450	7	96	theme	organic	1121:1127	arg1	matter					1129:1134	organic matter	1121:1134	organic matter (OM; 94.1 vs. 85.1% of DM)	1121:1161	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	8	97	theme	DM	1545:1546	arg1	%					1540:1540	63.2 vs. 40.6%	1527:1540	63.2 vs. 40.6% of DM	1527:1546	Sugarcane bagasse also had greater physically effective NDF (total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves, peNDF8; 63.2 vs. 40.6% of DM) and undegraded NDF after 288 h of incubation (uNDF288; 35.5 vs. 21.2% of DM) contents than WS.
32684450	8	97	theme	DM	1545:1546	arg1	DM					1545:1546	DM	1545:1546	DM	1545:1546	Sugarcane bagasse also had greater physically effective NDF (total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves, peNDF8; 63.2 vs. 40.6% of DM) and undegraded NDF after 288 h of incubation (uNDF288; 35.5 vs. 21.2% of DM) contents than WS.
32684450	2	98	theme	conventional	319:330	arg1	sources					332:338	other conventional sources	313:338	other conventional sources of forage	313:348	It is generally less costly compared with other conventional sources of forage.
32684450	9	99	from	inclusion	1848:1856	arg1	diets					1865:1869	the diets	1861:1869	the diets	1861:1869	The undegraded NDF after 30 h of incubation (uNDF30) content was similar for all diets; however, peNDF8 concentration and proportion of long particles (retained on a 19-mm sieve) increased linearly as SB inclusion in the diets increased.
32684450	13	100	theme	feed	2291:2294	arg1	intake					2332:2337	1.44 kg ECM yield/kg DM intake	2308:2337	1.44 kg ECM yield/kg DM intake	2308:2337	Milk yield (37.0 kg/d), energy-corrected milk yield (ECM; 38.2 kg/d), feed efficiency (1.44 kg ECM yield/kg DM intake), and milk composition (fat, 3.89%; true protein, 2.90%) did not differ among diets.
32684450	13	100	theme	feed	2291:2294	arg1	efficiency					2296:2305	feed efficiency	2291:2305	feed efficiency (1.44 kg ECM yield/kg DM intake)	2291:2338	Milk yield (37.0 kg/d), energy-corrected milk yield (ECM; 38.2 kg/d), feed efficiency (1.44 kg ECM yield/kg DM intake), and milk composition (fat, 3.89%; true protein, 2.90%) did not differ among diets.
32684450	13	101	dep	ECM	2274:2276	arg1	kg/d					2284:2287	38.2 kg/d	2279:2287	ECM; 38.2 kg/d	2274:2287	Milk yield (37.0 kg/d), energy-corrected milk yield (ECM; 38.2 kg/d), feed efficiency (1.44 kg ECM yield/kg DM intake), and milk composition (fat, 3.89%; true protein, 2.90%) did not differ among diets.
32684450	16	102	with	diets	2775:2779	arg1	concentrations					2801:2814	similar uNDF30 concentrations	2786:2814	similar uNDF30 concentrations	2786:2814	The results indicate that replacement of WS with increasing levels of SB in low-forage diets with similar uNDF30 concentrations did not affect performance of mid-lactation dairy cows.
32684450	7	103	theme	Sugarcane	1091:1099	arg1	bagasse					1101:1107	Sugarcane bagasse	1091:1107	Sugarcane bagasse	1091:1107	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	8	104	dep	NDF	1445:1447	arg1	peNDF8					1519:1524	peNDF8	1519:1524	peNDF8	1519:1524	Sugarcane bagasse also had greater physically effective NDF (total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves, peNDF8; 63.2 vs. 40.6% of DM) and undegraded NDF after 288 h of incubation (uNDF288; 35.5 vs. 21.2% of DM) contents than WS.
32684450	8	104	dep	NDF	1445:1447	arg1	NDF					1464:1466	total dietary NDF	1450:1466	total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves	1450:1516	Sugarcane bagasse also had greater physically effective NDF (total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves, peNDF8; 63.2 vs. 40.6% of DM) and undegraded NDF after 288 h of incubation (uNDF288; 35.5 vs. 21.2% of DM) contents than WS.
32684450	12	105	theme	OM	2167:2168	arg1	digestibilities					2148:2162	Total-tract digestibilities	2136:2162	Total-tract digestibilities of OM, CP, and NDF	2136:2181	Total-tract digestibilities of OM, CP, and NDF decreased linearly as SB replaced WS.
32684450	6	106	dep	9SB	1004:1006	arg1	2					1001:1001	2	1001:1001	2	1001:1001	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	7	107	dep	protein	1331:1337	arg1	CP					1340:1341	CP	1340:1341	CP; 2.63 vs. 3.72% of DM	1340:1363	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	4	108	theme	dairy	580:584	arg1	cows					586:589	mid-lactation dairy cows	566:589	mid-lactation dairy cows on milk production and fatty acid profile, intake, digestibility, chewing activity, and ruminal fermentation	566:698	This study evaluated the effects of replacing WS with SB in diets offered to mid-lactation dairy cows on milk production and fatty acid profile, intake, digestibility, chewing activity, and ruminal fermentation.
32684450	7	109	theme	DM	1212:1213	arg1	DM					1212:1213	DM	1212:1213	DM	1212:1213	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	7	109	theme	DM	1212:1213	arg1	%					1207:1207	86.2 vs. 76.4%	1194:1207	NDF; 86.2 vs. 76.4% of DM	1189:1213	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	7	110	theme	lignin	1271:1276	arg1	concentration					1301:1313	greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration	1113:1313	greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration	1113:1313	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	6	111	theme	%	1037:1037	arg1	WS					1039:1040	18% WS	1035:1040	18% WS	1035:1040	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	5	112	theme	±	748:748	arg1	deviation					759:767	mean ± standard deviation	743:767	mean ± standard deviation	743:767	Nine multiparous Holstein cows averaging (mean ± standard deviation) 105 ± 12 d in milk, 42.1 ± 2.9 kg of milk/d, and 617 ± 59 kg of body weight were used in a replicated 3 × 3 Latin square with 21-d periods.
32684450	5	112	theme	±	748:748	arg1	105					770:772	105	770:772	105	770:772	Nine multiparous Holstein cows averaging (mean ± standard deviation) 105 ± 12 d in milk, 42.1 ± 2.9 kg of milk/d, and 617 ± 59 kg of body weight were used in a replicated 3 × 3 Latin square with 21-d periods.
32684450	14	113	theme	ruminal	2504:2510	arg1	pH					2512:2513	ruminal pH	2504:2513	ruminal pH (ruminocentesis, 4 h after feeding)	2504:2549	Increasing SB concentration of the diet linearly increased rumination time, but ruminal pH (ruminocentesis, 4 h after feeding) decreased.
32684450	8	114	theme	TMR	1487:1489	arg1	TMR					1487:1489	TMR	1487:1489	TMR	1487:1489	Sugarcane bagasse also had greater physically effective NDF (total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves, peNDF8; 63.2 vs. 40.6% of DM) and undegraded NDF after 288 h of incubation (uNDF288; 35.5 vs. 21.2% of DM) contents than WS.
32684450	8	114	theme	TMR	1487:1489	arg1	%					1482:1482	%	1482:1482	% of TMR	1482:1489	Sugarcane bagasse also had greater physically effective NDF (total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves, peNDF8; 63.2 vs. 40.6% of DM) and undegraded NDF after 288 h of incubation (uNDF288; 35.5 vs. 21.2% of DM) contents than WS.
32684450	5	115	theme	21-d	896:899	arg1	periods					901:907	21-d periods	896:907	21-d periods	896:907	Nine multiparous Holstein cows averaging (mean ± standard deviation) 105 ± 12 d in milk, 42.1 ± 2.9 kg of milk/d, and 617 ± 59 kg of body weight were used in a replicated 3 × 3 Latin square with 21-d periods.
32684450	9	116	theme	peNDF8	1741:1746	arg1	concentration					1748:1760	peNDF8 concentration	1741:1760	peNDF8 concentration	1741:1760	The undegraded NDF after 30 h of incubation (uNDF30) content was similar for all diets; however, peNDF8 concentration and proportion of long particles (retained on a 19-mm sieve) increased linearly as SB inclusion in the diets increased.
32684450	17	117	used	used	2899:2902	arg2	source					2915:2920	a fiber source	2907:2920	a fiber source	2907:2920	We conclude that SB can be used as a fiber source in diets fed to dairy cows in mid-lactation; however, the decrease in total-tract digestibility of diets may decrease lactational performance when fed to high-producing dairy cows.
32684450	17	117	used	used	2899:2902	arg2	SB					2889:2890	SB	2889:2890	SB	2889:2890	We conclude that SB can be used as a fiber source in diets fed to dairy cows in mid-lactation; however, the decrease in total-tract digestibility of diets may decrease lactational performance when fed to high-producing dairy cows.
32684450	6	118	theme	%	1026:1026	arg1	SB					1028:1029	9% SB	1025:1029	9% SB	1025:1029	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	7	119	dep	NDF	1189:1191	arg1	DM					1212:1213	DM	1212:1213	DM	1212:1213	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	7	119	dep	NDF	1189:1191	arg1	%					1207:1207	86.2 vs. 76.4%	1194:1207	NDF; 86.2 vs. 76.4% of DM	1189:1213	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	13	120	theme	milk	2262:2265	arg1	yield					2267:2271	energy-corrected milk yield	2245:2271	energy-corrected milk yield (ECM; 38.2 kg/d)	2245:2288	Milk yield (37.0 kg/d), energy-corrected milk yield (ECM; 38.2 kg/d), feed efficiency (1.44 kg ECM yield/kg DM intake), and milk composition (fat, 3.89%; true protein, 2.90%) did not differ among diets.
32684450	14	121	theme	rumination	2483:2492	arg1	time					2494:2497	rumination time	2483:2497	rumination time	2483:2497	Increasing SB concentration of the diet linearly increased rumination time, but ruminal pH (ruminocentesis, 4 h after feeding) decreased.
32684450	6	122	theme	%	983:983	arg1	SB					985:986	0% SB	982:986	0% SB	982:986	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	11	123	theme	NDF	1980:1982	arg1	Intakes					1949:1955	Intakes	1949:1955	Intakes of DM (26.53 kg/d) and NDF (8.58 kg/d)	1949:1994	Intakes of DM (26.53 kg/d) and NDF (8.58 kg/d) did not differ among the treatments, but intakes of OM and CP decreased, whereas ADF and uNDF288 intakes increased with SB inclusion level.
32684450	17	124	theme	fiber	2909:2913	arg1	source					2915:2920	a fiber source	2907:2920	a fiber source	2907:2920	We conclude that SB can be used as a fiber source in diets fed to dairy cows in mid-lactation; however, the decrease in total-tract digestibility of diets may decrease lactational performance when fed to high-producing dairy cows.
32684450	17	124	theme	fiber	2909:2913	arg1	SB					2889:2890	SB	2889:2890	SB	2889:2890	We conclude that SB can be used as a fiber source in diets fed to dairy cows in mid-lactation; however, the decrease in total-tract digestibility of diets may decrease lactational performance when fed to high-producing dairy cows.
32684450	3	125	dep	low-quality	469:479	arg1	forage					481:486	forage	481:486	forage	481:486	We hypothesized that SB could be used as a source of roughage for dairy cattle by replacing wheat straw (WS), another low-quality forage.
32684450	8	126	dep	NDF	1464:1466	arg1	%					1540:1540	63.2 vs. 40.6%	1527:1540	63.2 vs. 40.6% of DM	1527:1546	Sugarcane bagasse also had greater physically effective NDF (total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves, peNDF8; 63.2 vs. 40.6% of DM) and undegraded NDF after 288 h of incubation (uNDF288; 35.5 vs. 21.2% of DM) contents than WS.
32684450	8	126	dep	NDF	1464:1466	arg1	DM					1545:1546	DM	1545:1546	DM	1545:1546	Sugarcane bagasse also had greater physically effective NDF (total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves, peNDF8; 63.2 vs. 40.6% of DM) and undegraded NDF after 288 h of incubation (uNDF288; 35.5 vs. 21.2% of DM) contents than WS.
32684450	17	127	theme	high-producing	3076:3089	arg1	cows					3097:3100	high-producing dairy cows	3076:3100	high-producing dairy cows	3076:3100	We conclude that SB can be used as a fiber source in diets fed to dairy cows in mid-lactation; however, the decrease in total-tract digestibility of diets may decrease lactational performance when fed to high-producing dairy cows.
32684450	9	128	theme	particles	1785:1793	arg1	proportion					1766:1775	proportion	1766:1775	proportion	1766:1775	The undegraded NDF after 30 h of incubation (uNDF30) content was similar for all diets; however, peNDF8 concentration and proportion of long particles (retained on a 19-mm sieve) increased linearly as SB inclusion in the diets increased.
32684450	9	128	theme	particles	1785:1793	arg1	concentration					1748:1760	peNDF8 concentration	1741:1760	peNDF8 concentration	1741:1760	The undegraded NDF after 30 h of incubation (uNDF30) content was similar for all diets; however, peNDF8 concentration and proportion of long particles (retained on a 19-mm sieve) increased linearly as SB inclusion in the diets increased.
32684450	5	129	from	d	779:779	arg1	milk					784:787	milk	784:787	milk	784:787	Nine multiparous Holstein cows averaging (mean ± standard deviation) 105 ± 12 d in milk, 42.1 ± 2.9 kg of milk/d, and 617 ± 59 kg of body weight were used in a replicated 3 × 3 Latin square with 21-d periods.
32684450	7	130	theme	detergent	1172:1180	arg1	fiber					1182:1186	neutral detergent fiber	1164:1186	neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM)	1164:1214	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	13	131	theme	Milk	2221:2224	arg1	yield					2226:2230	Milk yield	2221:2230	Milk yield (37.0 kg/d)	2221:2242	Milk yield (37.0 kg/d), energy-corrected milk yield (ECM; 38.2 kg/d), feed efficiency (1.44 kg ECM yield/kg DM intake), and milk composition (fat, 3.89%; true protein, 2.90%) did not differ among diets.
32684450	13	131	theme	Milk	2221:2224	arg1	kg/d					2238:2241	37.0 kg/d	2233:2241	37.0 kg/d	2233:2241	Milk yield (37.0 kg/d), energy-corrected milk yield (ECM; 38.2 kg/d), feed efficiency (1.44 kg ECM yield/kg DM intake), and milk composition (fat, 3.89%; true protein, 2.90%) did not differ among diets.
32684450	8	132	theme	DM	1622:1623	arg1	DM					1622:1623	DM	1622:1623	DM	1622:1623	Sugarcane bagasse also had greater physically effective NDF (total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves, peNDF8; 63.2 vs. 40.6% of DM) and undegraded NDF after 288 h of incubation (uNDF288; 35.5 vs. 21.2% of DM) contents than WS.
32684450	8	132	theme	DM	1622:1623	arg1	%					1617:1617	35.5 vs. 21.2%	1604:1617	uNDF288; 35.5 vs. 21.2% of DM	1595:1623	Sugarcane bagasse also had greater physically effective NDF (total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves, peNDF8; 63.2 vs. 40.6% of DM) and undegraded NDF after 288 h of incubation (uNDF288; 35.5 vs. 21.2% of DM) contents than WS.
32684450	17	133	theme	dairy	3091:3095	arg1	cows					3097:3100	high-producing dairy cows	3076:3100	high-producing dairy cows	3076:3100	We conclude that SB can be used as a fiber source in diets fed to dairy cows in mid-lactation; however, the decrease in total-tract digestibility of diets may decrease lactational performance when fed to high-producing dairy cows.
32684450	8	134	theme	effective	1435:1443	arg1	NDF					1445:1447	physically effective NDF	1424:1447	physically effective NDF (total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves, peNDF8; 63.2 vs. 40.6% of DM)	1424:1547	Sugarcane bagasse also had greater physically effective NDF (total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves, peNDF8; 63.2 vs. 40.6% of DM) and undegraded NDF after 288 h of incubation (uNDF288; 35.5 vs. 21.2% of DM) contents than WS.
32684450	16	135	theme	cows	2866:2869	arg1	performance					2831:2841	performance	2831:2841	performance of mid-lactation dairy cows	2831:2869	The results indicate that replacement of WS with increasing levels of SB in low-forage diets with similar uNDF30 concentrations did not affect performance of mid-lactation dairy cows.
32684450	6	136	contain	containing	1014:1023	arg2	WS					1039:1040	18% WS	1035:1040	18% WS	1035:1040	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	6	136	contain	containing	1014:1023	arg1	diet					1009:1012	diet	1009:1012	diet containing 9% SB and 18% WS	1009:1040	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	6	136	contain	containing	1014:1023	arg2	SB					1028:1029	9% SB	1025:1029	9% SB	1025:1029	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	6	136	contain	containing	1014:1023	arg1	0SB					961:963	(1) 0SB	957:963	(1) 0SB	957:963	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	0	137	theme	Milk	104:107	arg1	cows					98:101	mid-lactation dairy cows	78:101	mid-lactation dairy cows: Milk production, digestibility, and chewing behavior	78:155	Substitution of wheat straw with sugarcane bagasse in low-forage diets fed to mid-lactation dairy cows: Milk production, digestibility, and chewing behavior.
32684450	0	137	theme	Milk	104:107	arg1	production					109:118	Milk production	104:118	Milk production	104:118	Substitution of wheat straw with sugarcane bagasse in low-forage diets fed to mid-lactation dairy cows: Milk production, digestibility, and chewing behavior.
32684450	4	138	theme	fatty	614:618	arg1	profile					625:631	fatty acid profile	614:631	fatty acid profile	614:631	This study evaluated the effects of replacing WS with SB in diets offered to mid-lactation dairy cows on milk production and fatty acid profile, intake, digestibility, chewing activity, and ruminal fermentation.
32684450	0	139	theme	dairy	92:96	arg1	digestibility					121:133	digestibility	121:133	digestibility	121:133	Substitution of wheat straw with sugarcane bagasse in low-forage diets fed to mid-lactation dairy cows: Milk production, digestibility, and chewing behavior.
32684450	0	139	theme	dairy	92:96	arg1	cows					98:101	mid-lactation dairy cows	78:101	mid-lactation dairy cows: Milk production, digestibility, and chewing behavior	78:155	Substitution of wheat straw with sugarcane bagasse in low-forage diets fed to mid-lactation dairy cows: Milk production, digestibility, and chewing behavior.
32684450	0	139	theme	dairy	92:96	arg1	production					109:118	Milk production	104:118	Milk production	104:118	Substitution of wheat straw with sugarcane bagasse in low-forage diets fed to mid-lactation dairy cows: Milk production, digestibility, and chewing behavior.
32684450	0	139	theme	dairy	92:96	arg1	behavior					148:155	chewing behavior	140:155	chewing behavior	140:155	Substitution of wheat straw with sugarcane bagasse in low-forage diets fed to mid-lactation dairy cows: Milk production, digestibility, and chewing behavior.
32684450	7	140	theme	45.2	1253:1256	arg1	%					1257:1257	62.9 vs. 45.2%	1244:1257	%	1257:1257	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	7	140	theme	45.2	1253:1256	arg1	DM					1262:1263	DM	1262:1263	DM	1262:1263	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	7	141	theme	DM	1159:1160	arg1	DM					1159:1160	DM	1159:1160	DM	1159:1160	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	7	141	theme	DM	1159:1160	arg1	%					1154:1154	94.1 vs. 85.1%	1141:1154	%	1154:1154	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	16	142	theme	SB	2758:2759	arg1	levels					2748:2753	levels	2748:2753	levels of SB	2748:2759	The results indicate that replacement of WS with increasing levels of SB in low-forage diets with similar uNDF30 concentrations did not affect performance of mid-lactation dairy cows.
32684450	14	143	theme	Increasing	2424:2433	arg1	concentration					2438:2450	Increasing SB concentration	2424:2450	Increasing SB concentration of the diet	2424:2462	Increasing SB concentration of the diet linearly increased rumination time, but ruminal pH (ruminocentesis, 4 h after feeding) decreased.
32684450	3	144	used	used	384:387	arg2	SB					372:373	SB	372:373	SB	372:373	We hypothesized that SB could be used as a source of roughage for dairy cattle by replacing wheat straw (WS), another low-quality forage.
32684450	3	144	used	used	384:387	arg2	source					394:399	a source	392:399	a source of roughage for dairy cattle	392:428	We hypothesized that SB could be used as a source of roughage for dairy cattle by replacing wheat straw (WS), another low-quality forage.
32684450	4	145	from	effects	514:520	arg1	diets					549:553	diets	549:553	diets offered to mid-lactation dairy cows on milk production and fatty acid profile, intake, digestibility, chewing activity, and ruminal fermentation	549:698	This study evaluated the effects of replacing WS with SB in diets offered to mid-lactation dairy cows on milk production and fatty acid profile, intake, digestibility, chewing activity, and ruminal fermentation.
32684450	17	146	theme	dairy	2938:2942	arg1	cows					2944:2947	dairy cows	2938:2947	dairy cows	2938:2947	We conclude that SB can be used as a fiber source in diets fed to dairy cows in mid-lactation; however, the decrease in total-tract digestibility of diets may decrease lactational performance when fed to high-producing dairy cows.
32684450	7	147	theme	62.9	1244:1247	arg1	%					1257:1257	62.9 vs. 45.2%	1244:1257	%	1257:1257	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	7	147	theme	62.9	1244:1247	arg1	DM					1262:1263	DM	1262:1263	DM	1262:1263	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	4	148	theme	ruminal	679:685	arg1	fermentation					687:698	ruminal fermentation	679:698	ruminal fermentation	679:698	This study evaluated the effects of replacing WS with SB in diets offered to mid-lactation dairy cows on milk production and fatty acid profile, intake, digestibility, chewing activity, and ruminal fermentation.
32684450	5	149	with	×	874:874	arg1	periods					901:907	21-d periods	896:907	21-d periods	896:907	Nine multiparous Holstein cows averaging (mean ± standard deviation) 105 ± 12 d in milk, 42.1 ± 2.9 kg of milk/d, and 617 ± 59 kg of body weight were used in a replicated 3 × 3 Latin square with 21-d periods.
32684450	8	150	theme	dietary	1456:1462	arg1	peNDF8					1519:1524	peNDF8	1519:1524	peNDF8	1519:1524	Sugarcane bagasse also had greater physically effective NDF (total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves, peNDF8; 63.2 vs. 40.6% of DM) and undegraded NDF after 288 h of incubation (uNDF288; 35.5 vs. 21.2% of DM) contents than WS.
32684450	8	150	theme	dietary	1456:1462	arg1	NDF					1464:1466	total dietary NDF	1450:1466	total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves	1450:1516	Sugarcane bagasse also had greater physically effective NDF (total dietary NDF multiplied by % of TMR on the 8-mm + 19-mm sieves, peNDF8; 63.2 vs. 40.6% of DM) and undegraded NDF after 288 h of incubation (uNDF288; 35.5 vs. 21.2% of DM) contents than WS.
32684450	6	151	theme	%	1085:1085	arg1	WS					1087:1088	9% WS	1084:1088	9% WS	1084:1088	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	17	152	theme	diets	3021:3025	arg1	digestibility					3004:3016	total-tract digestibility	2992:3016	total-tract digestibility of diets	2992:3025	We conclude that SB can be used as a fiber source in diets fed to dairy cows in mid-lactation; however, the decrease in total-tract digestibility of diets may decrease lactational performance when fed to high-producing dairy cows.
32684450	5	153	theme	milk/d	807:812	arg1	milk/d					807:812	milk/d	807:812	milk/d	807:812	Nine multiparous Holstein cows averaging (mean ± standard deviation) 105 ± 12 d in milk, 42.1 ± 2.9 kg of milk/d, and 617 ± 59 kg of body weight were used in a replicated 3 × 3 Latin square with 21-d periods.
32684450	5	153	theme	milk/d	807:812	arg1	kg					801:802	kg	801:802	kg	801:802	Nine multiparous Holstein cows averaging (mean ± standard deviation) 105 ± 12 d in milk, 42.1 ± 2.9 kg of milk/d, and 617 ± 59 kg of body weight were used in a replicated 3 × 3 Latin square with 21-d periods.
32684450	15	154	theme	fatty	2577:2581	arg1	concentration					2588:2600	Total volatile fatty acid concentration	2562:2600	Total volatile fatty acid concentration	2562:2600	Total volatile fatty acid concentration increased linearly, whereas acetate:propionate decreased linearly, as SB replaced WS.
32684450	11	155	theme	inclusion	2119:2127	arg1	level					2129:2133	SB inclusion level	2116:2133	SB inclusion level	2116:2133	Intakes of DM (26.53 kg/d) and NDF (8.58 kg/d) did not differ among the treatments, but intakes of OM and CP decreased, whereas ADF and uNDF288 intakes increased with SB inclusion level.
32684450	2	156	theme	forage	343:348	arg1	sources					332:338	other conventional sources	313:338	other conventional sources of forage	313:348	It is generally less costly compared with other conventional sources of forage.
32684450	0	157	theme	straw	22:26	arg1	Substitution					0:11	Substitution	0:11	Substitution of wheat straw with sugarcane bagasse in low-forage diets	0:69	Substitution of wheat straw with sugarcane bagasse in low-forage diets fed to mid-lactation dairy cows: Milk production, digestibility, and chewing behavior.
32684450	16	158	theme	uNDF30	2794:2799	arg1	concentrations					2801:2814	similar uNDF30 concentrations	2786:2814	similar uNDF30 concentrations	2786:2814	The results indicate that replacement of WS with increasing levels of SB in low-forage diets with similar uNDF30 concentrations did not affect performance of mid-lactation dairy cows.
32684450	7	159	theme	fiber	1232:1236	arg1	concentration					1301:1313	greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration	1113:1313	greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration	1113:1313	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	13	160	theme	ECM	2316:2318	arg1	intake					2332:2337	1.44 kg ECM yield/kg DM intake	2308:2337	1.44 kg ECM yield/kg DM intake	2308:2337	Milk yield (37.0 kg/d), energy-corrected milk yield (ECM; 38.2 kg/d), feed efficiency (1.44 kg ECM yield/kg DM intake), and milk composition (fat, 3.89%; true protein, 2.90%) did not differ among diets.
32684450	13	160	theme	ECM	2316:2318	arg1	efficiency					2296:2305	feed efficiency	2291:2305	feed efficiency (1.44 kg ECM yield/kg DM intake)	2291:2338	Milk yield (37.0 kg/d), energy-corrected milk yield (ECM; 38.2 kg/d), feed efficiency (1.44 kg ECM yield/kg DM intake), and milk composition (fat, 3.89%; true protein, 2.90%) did not differ among diets.
32684450	4	161	theme	chewing	657:663	arg1	activity					665:672	chewing activity	657:672	chewing activity	657:672	This study evaluated the effects of replacing WS with SB in diets offered to mid-lactation dairy cows on milk production and fatty acid profile, intake, digestibility, chewing activity, and ruminal fermentation.
32684450	3	162	theme	dairy	417:421	arg1	cattle					423:428	dairy cattle	417:428	dairy cattle	417:428	We hypothesized that SB could be used as a source of roughage for dairy cattle by replacing wheat straw (WS), another low-quality forage.
32684450	6	163	theme	%	1075:1075	arg1	SB					1077:1078	18% SB	1073:1078	18% SB	1073:1078	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	7	164	dep	matter	1129:1134	arg1	%					1292:1292	19.9 vs. 10.3%	1279:1292	19.9 vs. 10.3% of DM	1279:1298	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	7	164	dep	matter	1129:1134	arg1	OM					1137:1138	OM	1137:1138	OM; 94.1 vs. 85.1% of DM	1137:1160	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	7	164	dep	matter	1129:1134	arg1	DM					1297:1298	DM	1297:1298	DM	1297:1298	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	4	165	theme	mid-lactation	566:578	arg1	cows					586:589	mid-lactation dairy cows	566:589	mid-lactation dairy cows on milk production and fatty acid profile, intake, digestibility, chewing activity, and ruminal fermentation	566:698	This study evaluated the effects of replacing WS with SB in diets offered to mid-lactation dairy cows on milk production and fatty acid profile, intake, digestibility, chewing activity, and ruminal fermentation.
32684450	7	166	theme	acid	1217:1220	arg1	fiber					1232:1236	acid detergent fiber	1217:1236	acid detergent fiber (ADF; 62.9 vs. 45.2% of DM)	1217:1264	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	13	167	theme	DM	2329:2330	arg1	intake					2332:2337	1.44 kg ECM yield/kg DM intake	2308:2337	1.44 kg ECM yield/kg DM intake	2308:2337	Milk yield (37.0 kg/d), energy-corrected milk yield (ECM; 38.2 kg/d), feed efficiency (1.44 kg ECM yield/kg DM intake), and milk composition (fat, 3.89%; true protein, 2.90%) did not differ among diets.
32684450	13	167	theme	DM	2329:2330	arg1	efficiency					2296:2305	feed efficiency	2291:2305	feed efficiency (1.44 kg ECM yield/kg DM intake)	2291:2338	Milk yield (37.0 kg/d), energy-corrected milk yield (ECM; 38.2 kg/d), feed efficiency (1.44 kg ECM yield/kg DM intake), and milk composition (fat, 3.89%; true protein, 2.90%) did not differ among diets.
32684450	3	168	theme	roughage	404:411	arg1	SB					372:373	SB	372:373	SB	372:373	We hypothesized that SB could be used as a source of roughage for dairy cattle by replacing wheat straw (WS), another low-quality forage.
32684450	3	168	theme	roughage	404:411	arg1	source					394:399	a source	392:399	a source of roughage for dairy cattle	392:428	We hypothesized that SB could be used as a source of roughage for dairy cattle by replacing wheat straw (WS), another low-quality forage.
32684450	5	169	theme	multiparous	706:716	arg1	cows					727:730	Nine multiparous Holstein cows	701:730	Nine multiparous Holstein cows averaging (mean ± standard deviation) 105 ± 12 d in milk, 42.1 ± 2.9 kg of milk/d, and 617 ± 59 kg of body weight	701:844	Nine multiparous Holstein cows averaging (mean ± standard deviation) 105 ± 12 d in milk, 42.1 ± 2.9 kg of milk/d, and 617 ± 59 kg of body weight were used in a replicated 3 × 3 Latin square with 21-d periods.
32684450	7	170	theme	85.1	1150:1153	arg1	DM					1159:1160	DM	1159:1160	DM	1159:1160	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	7	170	theme	85.1	1150:1153	arg1	%					1154:1154	94.1 vs. 85.1%	1141:1154	%	1154:1154	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	5	171	theme	replicated	861:870	arg1	×					874:874	3 × 3	872:876	a replicated 3 × 3 Latin square with 21-d periods	859:907	Nine multiparous Holstein cows averaging (mean ± standard deviation) 105 ± 12 d in milk, 42.1 ± 2.9 kg of milk/d, and 617 ± 59 kg of body weight were used in a replicated 3 × 3 Latin square with 21-d periods.
32684450	6	172	theme	matter	944:949	arg1	%					927:927	%	927:927	% of dietary dry matter	927:949	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	6	172	theme	matter	944:949	arg1	DM					952:953	DM	952:953	DM	952:953	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	6	172	theme	matter	944:949	arg1	matter					944:949	dietary dry matter	932:949	dietary dry matter	932:949	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	6	173	contain	containing	971:980	arg2	SB					985:986	0% SB	982:986	0% SB	982:986	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	6	173	contain	containing	971:980	arg1	diet					966:969	diet	966:969	diet containing 0% SB and 27% WS	966:997	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	6	173	contain	containing	971:980	arg2	WS					996:997	27% WS	992:997	27% WS	992:997	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	6	173	contain	containing	971:980	arg1	0SB					961:963	(1) 0SB	957:963	(1) 0SB	957:963	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	13	174	theme	milk	2345:2348	arg1	composition					2350:2360	milk composition	2345:2360	milk composition (fat, 3.89%; true protein, 2.90%)	2345:2394	Milk yield (37.0 kg/d), energy-corrected milk yield (ECM; 38.2 kg/d), feed efficiency (1.44 kg ECM yield/kg DM intake), and milk composition (fat, 3.89%; true protein, 2.90%) did not differ among diets.
32684450	12	175	theme	Total-tract	2136:2146	arg1	digestibilities					2148:2162	Total-tract digestibilities	2136:2162	Total-tract digestibilities of OM, CP, and NDF	2136:2181	Total-tract digestibilities of OM, CP, and NDF decreased linearly as SB replaced WS.
32684450	3	176	theme	wheat	443:447	arg1	low-quality					469:479	another low-quality	461:479	another low-quality forage	461:486	We hypothesized that SB could be used as a source of roughage for dairy cattle by replacing wheat straw (WS), another low-quality forage.
32684450	3	176	theme	wheat	443:447	arg1	WS					456:457	WS	456:457	WS	456:457	We hypothesized that SB could be used as a source of roughage for dairy cattle by replacing wheat straw (WS), another low-quality forage.
32684450	3	176	theme	wheat	443:447	arg1	straw					449:453	wheat straw	443:453	wheat straw (WS)	443:458	We hypothesized that SB could be used as a source of roughage for dairy cattle by replacing wheat straw (WS), another low-quality forage.
32684450	16	177	theme	mid-lactation	2846:2858	arg1	cows					2866:2869	mid-lactation dairy cows	2846:2869	mid-lactation dairy cows	2846:2869	The results indicate that replacement of WS with increasing levels of SB in low-forage diets with similar uNDF30 concentrations did not affect performance of mid-lactation dairy cows.
32684450	7	178	theme	94.1	1141:1144	arg1	DM					1159:1160	DM	1159:1160	DM	1159:1160	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	7	178	theme	94.1	1141:1144	arg1	%					1154:1154	94.1 vs. 85.1%	1141:1154	%	1154:1154	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	5	179	theme	body	834:837	arg1	weight					839:844	body weight	834:844	body weight	834:844	Nine multiparous Holstein cows averaging (mean ± standard deviation) 105 ± 12 d in milk, 42.1 ± 2.9 kg of milk/d, and 617 ± 59 kg of body weight were used in a replicated 3 × 3 Latin square with 21-d periods.
32684450	7	180	theme	crude	1325:1329	arg1	protein					1331:1337	less crude protein	1320:1337	less crude protein (CP; 2.63 vs. 3.72% of DM) concentration	1320:1378	Sugarcane bagasse had greater organic matter (OM; 94.1 vs. 85.1% of DM), neutral detergent fiber (NDF; 86.2 vs. 76.4% of DM), acid detergent fiber (ADF; 62.9 vs. 45.2% of DM), and lignin (19.9 vs. 10.3% of DM) concentration, but less crude protein (CP; 2.63 vs. 3.72% of DM) concentration than WS.
32684450	6	181	theme	dietary	932:938	arg1	matter					944:949	dietary dry matter	932:949	dietary dry matter	932:949	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	2	182	dep	sources	332:338	arg1	is					274:275	is	274:275	is generally less costly compared with other conventional sources of forage	274:348	It is generally less costly compared with other conventional sources of forage.
32684450	9	183	theme	incubation	1677:1686	arg1	content					1697:1703	incubation (uNDF30) content	1677:1703	incubation (uNDF30) content	1677:1703	The undegraded NDF after 30 h of incubation (uNDF30) content was similar for all diets; however, peNDF8 concentration and proportion of long particles (retained on a 19-mm sieve) increased linearly as SB inclusion in the diets increased.
32684450	1	184	theme	roughage	198:205	arg1	source					207:212	a low-quality roughage source	184:212	a low-quality roughage source that is often plentiful during times of forage shortage	184:268	Sugarcane bagasse (SB) is a low-quality roughage source that is often plentiful during times of forage shortage.
32684450	1	184	theme	roughage	198:205	arg1	bagasse					168:174	Sugarcane bagasse	158:174	Sugarcane bagasse (SB)	158:179	Sugarcane bagasse (SB) is a low-quality roughage source that is often plentiful during times of forage shortage.
32684450	1	184	theme	roughage	198:205	arg1	plentiful					228:236	plentiful	228:236	plentiful	228:236	Sugarcane bagasse (SB) is a low-quality roughage source that is often plentiful during times of forage shortage.
32684450	6	185	contain	containing	1062:1071	arg2	WS					1087:1088	9% WS	1084:1088	9% WS	1084:1088	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	6	185	contain	containing	1062:1071	arg1	0SB					961:963	(1) 0SB	957:963	(1) 0SB	957:963	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	6	185	contain	containing	1062:1071	arg1	diet					966:969	diet	966:969	diet containing 0% SB and 27% WS	966:997	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	6	185	contain	containing	1062:1071	arg2	SB					1077:1078	18% SB	1073:1078	18% SB	1073:1078	Treatments were (% of dietary dry matter, DM): (1) 0SB, diet containing 0% SB and 27% WS, (2) 9SB, diet containing 9% SB and 18% WS, and (3) 18SB, diet containing 18% SB and 9% WS.
32684450	5	186	dep	×	874:874	arg1	square					884:889	Latin square	878:889	a replicated 3 × 3 Latin square with 21-d periods	859:907	Nine multiparous Holstein cows averaging (mean ± standard deviation) 105 ± 12 d in milk, 42.1 ± 2.9 kg of milk/d, and 617 ± 59 kg of body weight were used in a replicated 3 × 3 Latin square with 21-d periods.
33915494	8	0	theme	ratio	977:981	arg1	abundance					1021:1029	the abundance	1017:1029	the abundance of Actinobacteriota	1017:1049	In addition, juglone altered microbial diversity and gut microbiota composition, including the enhancement of the ratio of Firmicutes to Bacteroidota and the abundance of Actinobacteriota, and decrease of the abundance of Verrucomicrobiota.
33915494	8	0	theme	ratio	977:981	arg1	enhancement					958:968	the enhancement	954:968	the enhancement of the ratio of Firmicutes to Bacteroidota	954:1011	In addition, juglone altered microbial diversity and gut microbiota composition, including the enhancement of the ratio of Firmicutes to Bacteroidota and the abundance of Actinobacteriota, and decrease of the abundance of Verrucomicrobiota.
33915494	1	1	theme	Juglans	146:152	arg1	mandshurica					154:164	Juglans mandshurica	146:164	Juglans mandshurica	146:164	Juglone, mainly isolates from the green walnut husks of Juglans mandshurica, exhibits anti-cancer and anti-inflammaroty activities.
33915494	7	2	theme	protein	834:840	arg1	expression					842:851	the protein expression	830:851	the protein expression of IL-10	830:860	Juglone treatment significantly inhibited the protein levels of IL-6, TNF-α and IL-1β, improved the protein expression of IL-10.
33915494	8	3	theme	Verrucomicrobiota	1085:1101	arg1	abundance					1072:1080	the abundance	1068:1080	the abundance of Verrucomicrobiota	1068:1101	In addition, juglone altered microbial diversity and gut microbiota composition, including the enhancement of the ratio of Firmicutes to Bacteroidota and the abundance of Actinobacteriota, and decrease of the abundance of Verrucomicrobiota.
33915494	7	4	dep	inhibited	766:774	arg1	improved					821:828	improved	821:828	improved the protein expression of IL-10	821:860	Juglone treatment significantly inhibited the protein levels of IL-6, TNF-α and IL-1β, improved the protein expression of IL-10.
33915494	8	5	theme	microbiota	920:929	arg1	composition					931:941	gut microbiota composition	916:941	gut microbiota composition	916:941	In addition, juglone altered microbial diversity and gut microbiota composition, including the enhancement of the ratio of Firmicutes to Bacteroidota and the abundance of Actinobacteriota, and decrease of the abundance of Verrucomicrobiota.
33915494	1	6	theme	mandshurica	154:164	arg1	husks					137:141	the green walnut husks	120:141	the green walnut husks of Juglans mandshurica	120:164	Juglone, mainly isolates from the green walnut husks of Juglans mandshurica, exhibits anti-cancer and anti-inflammaroty activities.
33915494	8	7	theme	Firmicutes	986:995	arg1	ratio					977:981	the ratio	973:981	the ratio of Firmicutes to Bacteroidota	973:1011	In addition, juglone altered microbial diversity and gut microbiota composition, including the enhancement of the ratio of Firmicutes to Bacteroidota and the abundance of Actinobacteriota, and decrease of the abundance of Verrucomicrobiota.
33915494	6	8	dep	decrease	620:627	arg1	improvement					662:672	improvement	662:672	improvement	662:672	The disease activity index (DAI) decrease and pathological characteristics improvement demonstrated that the UC in mice was alleviated by juglone.
33915494	10	9	theme	Th17	1265:1268	arg1	development					1270:1280	Th17 development	1265:1280	Th17 development	1265:1280	Furthermore, juglone inhibited Th17 development and increased Treg generation, beneficial to Th17/Treg balance.
33915494	9	10	theme	Juglone	1104:1110	arg1	treatment					1112:1120	Juglone treatment	1104:1120	Juglone treatment	1104:1120	Juglone treatment also inhibited the protein expressions of IL-6, STAT3 and RORγt, meanwhile improved the protein level of FOXP3.
33915494	10	11	theme	Treg	1296:1299	arg1	generation					1301:1310	increased Treg generation	1286:1310	increased Treg generation	1286:1310	Furthermore, juglone inhibited Th17 development and increased Treg generation, beneficial to Th17/Treg balance.
33915494	1	12	from	husks	137:141	arg1	Juglone					90:96	Juglone	90:96	Juglone	90:96	Juglone, mainly isolates from the green walnut husks of Juglans mandshurica, exhibits anti-cancer and anti-inflammaroty activities.
33915494	1	12	from	husks	137:141	arg1	isolates					106:113	isolates	106:113	mainly isolates from the green walnut husks of Juglans mandshurica	99:164	Juglone, mainly isolates from the green walnut husks of Juglans mandshurica, exhibits anti-cancer and anti-inflammaroty activities.
33915494	6	13	from	UC	696:697	arg1	mice					702:705	mice	702:705	mice	702:705	The disease activity index (DAI) decrease and pathological characteristics improvement demonstrated that the UC in mice was alleviated by juglone.
33915494	2	14	from	protection	230:239	arg1	UC					264:265	UC	264:265	UC	264:265	But its protection on ulcerative colitis (UC) has never been explored.
33915494	2	14	from	protection	230:239	arg1	colitis					255:261	ulcerative colitis	244:261	ulcerative colitis (UC)	244:266	But its protection on ulcerative colitis (UC) has never been explored.
33915494	0	15	from	balance	47:53	arg1	colitis					81:87	DSS-induced ulcerative colitis	58:87	DSS-induced ulcerative colitis	58:87	Juglone regulates gut microbiota and Th17/Treg balance in DSS-induced ulcerative colitis.
33915494	7	16	theme	IL-1β	814:818	arg1	levels					788:793	the protein levels	776:793	the protein levels of IL-6, TNF-α and IL-1β	776:818	Juglone treatment significantly inhibited the protein levels of IL-6, TNF-α and IL-1β, improved the protein expression of IL-10.
33915494	0	17	theme	Th17/Treg	37:45	arg1	balance					47:53	Th17/Treg balance	37:53	Th17/Treg balance	37:53	Juglone regulates gut microbiota and Th17/Treg balance in DSS-induced ulcerative colitis.
33915494	9	18	theme	IL-6	1164:1167	arg1	expressions					1149:1159	the protein expressions	1137:1159	the protein expressions of IL-6, STAT3 and RORγt	1137:1184	Juglone treatment also inhibited the protein expressions of IL-6, STAT3 and RORγt, meanwhile improved the protein level of FOXP3.
33915494	6	19	theme	pathological	633:644	arg1	characteristics					646:660	pathological characteristics	633:660	pathological characteristics	633:660	The disease activity index (DAI) decrease and pathological characteristics improvement demonstrated that the UC in mice was alleviated by juglone.
33915494	11	20	theme	Th17/Treg	1523:1531	arg1	homeostasis					1533:1543	Th17/Treg homeostasis	1523:1543	Th17/Treg homeostasis	1523:1543	Together, we herein provided the first evidence to support that juglone, especially the high dose, possibly protected mice against UC by modulating gut microbiota and restoring Th17/Treg homeostasis.
33915494	7	21	theme	TNF-α	804:808	arg1	levels					788:793	the protein levels	776:793	the protein levels of IL-6, TNF-α and IL-1β	776:818	Juglone treatment significantly inhibited the protein levels of IL-6, TNF-α and IL-1β, improved the protein expression of IL-10.
33915494	1	22	theme	anti-cancer	176:186	arg1	activities					210:219	anti-cancer and anti-inflammaroty activities	176:219	anti-cancer and anti-inflammaroty activities	176:219	Juglone, mainly isolates from the green walnut husks of Juglans mandshurica, exhibits anti-cancer and anti-inflammaroty activities.
33915494	9	23	theme	STAT3	1170:1174	arg1	expressions					1149:1159	the protein expressions	1137:1159	the protein expressions of IL-6, STAT3 and RORγt	1137:1184	Juglone treatment also inhibited the protein expressions of IL-6, STAT3 and RORγt, meanwhile improved the protein level of FOXP3.
33915494	3	24	theme	Th17/Treg	410:418	arg1	balance					420:426	Th17/Treg balance	410:426	Th17/Treg balance	410:426	In this study, we first evaluated whether juglone ameliorated UC, and investigated its effects on gut microbiota and Th17/Treg balance in DSS-induced UC mice model.
33915494	9	25	theme	protein	1141:1147	arg1	expressions					1149:1159	the protein expressions	1137:1159	the protein expressions of IL-6, STAT3 and RORγt	1137:1184	Juglone treatment also inhibited the protein expressions of IL-6, STAT3 and RORγt, meanwhile improved the protein level of FOXP3.
33915494	7	26	theme	IL-6	798:801	arg1	levels					788:793	the protein levels	776:793	the protein levels of IL-6, TNF-α and IL-1β	776:818	Juglone treatment significantly inhibited the protein levels of IL-6, TNF-α and IL-1β, improved the protein expression of IL-10.
33915494	3	27	theme	mice	446:449	arg1	model					451:455	DSS-induced UC mice model	431:455	DSS-induced UC mice model	431:455	In this study, we first evaluated whether juglone ameliorated UC, and investigated its effects on gut microbiota and Th17/Treg balance in DSS-induced UC mice model.
33915494	0	28	theme	gut	18:20	arg1	microbiota					22:31	gut microbiota	18:31	gut microbiota	18:31	Juglone regulates gut microbiota and Th17/Treg balance in DSS-induced ulcerative colitis.
33915494	9	29	theme	RORγt	1180:1184	arg1	expressions					1149:1159	the protein expressions	1137:1159	the protein expressions of IL-6, STAT3 and RORγt	1137:1184	Juglone treatment also inhibited the protein expressions of IL-6, STAT3 and RORγt, meanwhile improved the protein level of FOXP3.
33915494	7	30	theme	protein	780:786	arg1	levels					788:793	the protein levels	776:793	the protein levels of IL-6, TNF-α and IL-1β	776:818	Juglone treatment significantly inhibited the protein levels of IL-6, TNF-α and IL-1β, improved the protein expression of IL-10.
33915494	9	31	theme	FOXP3	1227:1231	arg1	level					1218:1222	the protein level	1206:1222	the protein level of FOXP3	1206:1231	Juglone treatment also inhibited the protein expressions of IL-6, STAT3 and RORγt, meanwhile improved the protein level of FOXP3.
33915494	3	32	theme	gut	391:393	arg1	microbiota					395:404	gut microbiota	391:404	gut microbiota	391:404	In this study, we first evaluated whether juglone ameliorated UC, and investigated its effects on gut microbiota and Th17/Treg balance in DSS-induced UC mice model.
33915494	2	33	theme	ulcerative	244:253	arg1	UC					264:265	UC	264:265	UC	264:265	But its protection on ulcerative colitis (UC) has never been explored.
33915494	2	33	theme	ulcerative	244:253	arg1	colitis					255:261	ulcerative colitis	244:261	ulcerative colitis (UC)	244:266	But its protection on ulcerative colitis (UC) has never been explored.
33915494	3	34	theme	DSS-induced	431:441	arg1	model					451:455	DSS-induced UC mice model	431:455	DSS-induced UC mice model	431:455	In this study, we first evaluated whether juglone ameliorated UC, and investigated its effects on gut microbiota and Th17/Treg balance in DSS-induced UC mice model.
33915494	7	35	theme	IL-10	856:860	arg1	expression					842:851	the protein expression	830:851	the protein expression of IL-10	830:860	Juglone treatment significantly inhibited the protein levels of IL-6, TNF-α and IL-1β, improved the protein expression of IL-10.
33915494	8	36	theme	Actinobacteriota	1034:1049	arg1	abundance					1021:1029	the abundance	1017:1029	the abundance of Actinobacteriota	1017:1049	In addition, juglone altered microbial diversity and gut microbiota composition, including the enhancement of the ratio of Firmicutes to Bacteroidota and the abundance of Actinobacteriota, and decrease of the abundance of Verrucomicrobiota.
33915494	8	36	theme	Actinobacteriota	1034:1049	arg1	enhancement					958:968	the enhancement	954:968	the enhancement of the ratio of Firmicutes to Bacteroidota	954:1011	In addition, juglone altered microbial diversity and gut microbiota composition, including the enhancement of the ratio of Firmicutes to Bacteroidota and the abundance of Actinobacteriota, and decrease of the abundance of Verrucomicrobiota.
33915494	3	37	theme	UC	443:444	arg1	model					451:455	DSS-induced UC mice model	431:455	DSS-induced UC mice model	431:455	In this study, we first evaluated whether juglone ameliorated UC, and investigated its effects on gut microbiota and Th17/Treg balance in DSS-induced UC mice model.
33915494	4	38	theme	%	505:505	arg1	DSS					507:509	2.7% DSS	502:509	2.7% DSS for seven days	502:524	The model was established by administrating 2.7% DSS for seven days.
33915494	8	39	theme	abundance	1072:1080	arg1	composition					931:941	gut microbiota composition	916:941	gut microbiota composition	916:941	In addition, juglone altered microbial diversity and gut microbiota composition, including the enhancement of the ratio of Firmicutes to Bacteroidota and the abundance of Actinobacteriota, and decrease of the abundance of Verrucomicrobiota.
33915494	8	39	theme	abundance	1072:1080	arg1	decrease					1056:1063	decrease	1056:1063	decrease of the abundance of Verrucomicrobiota	1056:1101	In addition, juglone altered microbial diversity and gut microbiota composition, including the enhancement of the ratio of Firmicutes to Bacteroidota and the abundance of Actinobacteriota, and decrease of the abundance of Verrucomicrobiota.
33915494	8	39	theme	abundance	1072:1080	arg1	diversity					902:910	microbial diversity	892:910	microbial diversity	892:910	In addition, juglone altered microbial diversity and gut microbiota composition, including the enhancement of the ratio of Firmicutes to Bacteroidota and the abundance of Actinobacteriota, and decrease of the abundance of Verrucomicrobiota.
33915494	10	40	theme	Th17/Treg	1327:1335	arg1	balance					1337:1343	Th17/Treg balance	1327:1343	Th17/Treg balance	1327:1343	Furthermore, juglone inhibited Th17 development and increased Treg generation, beneficial to Th17/Treg balance.
33915494	0	41	from	microbiota	22:31	arg1	colitis					81:87	DSS-induced ulcerative colitis	58:87	DSS-induced ulcerative colitis	58:87	Juglone regulates gut microbiota and Th17/Treg balance in DSS-induced ulcerative colitis.
33915494	11	42	theme	gut	1494:1496	arg1	microbiota					1498:1507	gut microbiota	1494:1507	gut microbiota	1494:1507	Together, we herein provided the first evidence to support that juglone, especially the high dose, possibly protected mice against UC by modulating gut microbiota and restoring Th17/Treg homeostasis.
33915494	10	43	theme	increased	1286:1294	arg1	generation					1301:1310	increased Treg generation	1286:1310	increased Treg generation	1286:1310	Furthermore, juglone inhibited Th17 development and increased Treg generation, beneficial to Th17/Treg balance.
33915494	6	44	theme	index	608:612	arg1	decrease					620:627	The disease activity index (DAI) decrease	587:627	The disease activity index (DAI) decrease	587:627	The disease activity index (DAI) decrease and pathological characteristics improvement demonstrated that the UC in mice was alleviated by juglone.
33915494	9	45	theme	protein	1210:1216	arg1	level					1218:1222	the protein level	1206:1222	the protein level of FOXP3	1206:1231	Juglone treatment also inhibited the protein expressions of IL-6, STAT3 and RORγt, meanwhile improved the protein level of FOXP3.
33915494	8	46	theme	gut	916:918	arg1	composition					931:941	gut microbiota composition	916:941	gut microbiota composition	916:941	In addition, juglone altered microbial diversity and gut microbiota composition, including the enhancement of the ratio of Firmicutes to Bacteroidota and the abundance of Actinobacteriota, and decrease of the abundance of Verrucomicrobiota.
33915494	1	47	theme	green	124:128	arg1	husks					137:141	the green walnut husks	120:141	the green walnut husks of Juglans mandshurica	120:164	Juglone, mainly isolates from the green walnut husks of Juglans mandshurica, exhibits anti-cancer and anti-inflammaroty activities.
33915494	6	48	theme	activity	599:606	arg1	DAI					615:617	DAI	615:617	DAI	615:617	The disease activity index (DAI) decrease and pathological characteristics improvement demonstrated that the UC in mice was alleviated by juglone.
33915494	6	48	theme	activity	599:606	arg1	index					608:612	disease activity index	591:612	The disease activity index (DAI) decrease	587:627	The disease activity index (DAI) decrease and pathological characteristics improvement demonstrated that the UC in mice was alleviated by juglone.
33915494	8	49	theme	microbial	892:900	arg1	diversity					902:910	microbial diversity	892:910	microbial diversity	892:910	In addition, juglone altered microbial diversity and gut microbiota composition, including the enhancement of the ratio of Firmicutes to Bacteroidota and the abundance of Actinobacteriota, and decrease of the abundance of Verrucomicrobiota.
33915494	1	50	theme	walnut	130:135	arg1	husks					137:141	the green walnut husks	120:141	the green walnut husks of Juglans mandshurica	120:164	Juglone, mainly isolates from the green walnut husks of Juglans mandshurica, exhibits anti-cancer and anti-inflammaroty activities.
33915494	6	51	theme	disease	591:597	arg1	DAI					615:617	DAI	615:617	DAI	615:617	The disease activity index (DAI) decrease and pathological characteristics improvement demonstrated that the UC in mice was alleviated by juglone.
33915494	6	51	theme	disease	591:597	arg1	index					608:612	disease activity index	591:612	The disease activity index (DAI) decrease	587:627	The disease activity index (DAI) decrease and pathological characteristics improvement demonstrated that the UC in mice was alleviated by juglone.
33915494	11	52	theme	first	1379:1383	arg1	evidence					1385:1392	the first evidence to support that juglone, especially the high dose, possibly protected mice against UC by modulating gut microbiota and restoring Th17/Treg homeostasis	1375:1543	the first evidence to support that juglone, especially the high dose, possibly protected mice against UC by modulating gut microbiota and restoring Th17/Treg homeostasis	1375:1543	Together, we herein provided the first evidence to support that juglone, especially the high dose, possibly protected mice against UC by modulating gut microbiota and restoring Th17/Treg homeostasis.
33915494	0	53	theme	ulcerative	70:79	arg1	colitis					81:87	DSS-induced ulcerative colitis	58:87	DSS-induced ulcerative colitis	58:87	Juglone regulates gut microbiota and Th17/Treg balance in DSS-induced ulcerative colitis.
33915494	1	54	theme	anti-inflammaroty	192:208	arg1	activities					210:219	anti-cancer and anti-inflammaroty activities	176:219	anti-cancer and anti-inflammaroty activities	176:219	Juglone, mainly isolates from the green walnut husks of Juglans mandshurica, exhibits anti-cancer and anti-inflammaroty activities.
33915494	11	55	theme	high	1434:1437	arg1	dose					1439:1442	the high dose	1430:1442	especially the high dose	1419:1442	Together, we herein provided the first evidence to support that juglone, especially the high dose, possibly protected mice against UC by modulating gut microbiota and restoring Th17/Treg homeostasis.
33915494	11	55	theme	high	1434:1437	arg1	juglone					1410:1416	juglone	1410:1416	juglone	1410:1416	Together, we herein provided the first evidence to support that juglone, especially the high dose, possibly protected mice against UC by modulating gut microbiota and restoring Th17/Treg homeostasis.
33915494	0	56	theme	DSS-induced	58:68	arg1	colitis					81:87	DSS-induced ulcerative colitis	58:87	DSS-induced ulcerative colitis	58:87	Juglone regulates gut microbiota and Th17/Treg balance in DSS-induced ulcerative colitis.
33915494	3	57	from	effects	380:386	arg1	balance					420:426	Th17/Treg balance	410:426	Th17/Treg balance	410:426	In this study, we first evaluated whether juglone ameliorated UC, and investigated its effects on gut microbiota and Th17/Treg balance in DSS-induced UC mice model.
33915494	3	57	from	effects	380:386	arg1	microbiota					395:404	gut microbiota	391:404	gut microbiota	391:404	In this study, we first evaluated whether juglone ameliorated UC, and investigated its effects on gut microbiota and Th17/Treg balance in DSS-induced UC mice model.
33915494	3	57	from	effects	380:386	arg1	model					451:455	DSS-induced UC mice model	431:455	DSS-induced UC mice model	431:455	In this study, we first evaluated whether juglone ameliorated UC, and investigated its effects on gut microbiota and Th17/Treg balance in DSS-induced UC mice model.
33915494	7	58	theme	Juglone	734:740	arg1	treatment					742:750	Juglone treatment	734:750	Juglone treatment	734:750	Juglone treatment significantly inhibited the protein levels of IL-6, TNF-α and IL-1β, improved the protein expression of IL-10.
33892069	8	0	theme	carrageenan	1363:1373	arg1	model					1375:1379	carrageenan model	1363:1379	carrageenan model in male Swiss mice	1363:1398	Oral treatments with OSAE and OSEO significantly inhibited the carrageenan-induced pleurisy in female Swiss mice, besides OSAE and OSEO significantly prevented paw edema (after 1, 2, and 4 h), mechanical hyperalgesia (after 3 and 4 h), and cold hyperalgesia 3 h after carrageenan model in male Swiss mice.
33892069	0	1	theme	oil	85:87	arg1	activity					18:25	Anti-inflammatory activity	0:25	Anti-inflammatory activity	0:25	Anti-inflammatory activity and chemical composition of aqueous extract and essential oil from leaves of Ocimum selloi Benth.
33892069	0	1	theme	oil	85:87	arg1	composition					40:50	chemical composition	31:50	chemical composition	31:50	Anti-inflammatory activity and chemical composition of aqueous extract and essential oil from leaves of Ocimum selloi Benth.
33892069	8	2	theme	male	1384:1387	arg1	mice					1395:1398	male Swiss mice	1384:1398	male Swiss mice	1384:1398	Oral treatments with OSAE and OSEO significantly inhibited the carrageenan-induced pleurisy in female Swiss mice, besides OSAE and OSEO significantly prevented paw edema (after 1, 2, and 4 h), mechanical hyperalgesia (after 3 and 4 h), and cold hyperalgesia 3 h after carrageenan model in male Swiss mice.
33892069	10	3	dep	CONCLUSION	1480:1489	arg1	evidenced					1503:1511	evidenced	1503:1511	evidenced	1503:1511	CONCLUSION The results evidenced the anti-inflammatory, anti-edematogenic, anti-hyperalgesic, and anti-nociceptive potentials of both materials obtained from leaves of O. selloi, mainly OSAE, supporting the popular use of this species.
33892069	8	4	theme	paw	1255:1257	arg1	edema					1259:1263	paw edema	1255:1263	paw edema (after 1, 2, and 4 h)	1255:1285	Oral treatments with OSAE and OSEO significantly inhibited the carrageenan-induced pleurisy in female Swiss mice, besides OSAE and OSEO significantly prevented paw edema (after 1, 2, and 4 h), mechanical hyperalgesia (after 3 and 4 h), and cold hyperalgesia 3 h after carrageenan model in male Swiss mice.
33892069	10	5	theme	materials	1614:1622	arg1	potentials					1595:1604	the anti-inflammatory, anti-edematogenic, anti-hyperalgesic, and anti-nociceptive potentials	1513:1604	the anti-inflammatory, anti-edematogenic, anti-hyperalgesic, and anti-nociceptive potentials of both materials obtained from leaves of O. selloi, mainly OSAE	1513:1669	CONCLUSION The results evidenced the anti-inflammatory, anti-edematogenic, anti-hyperalgesic, and anti-nociceptive potentials of both materials obtained from leaves of O. selloi, mainly OSAE, supporting the popular use of this species.
33892069	3	6	theme	inflammation	332:343	arg1	properties					345:354	inflammation properties	332:354	inflammation properties of O. selloi	332:367	This study is motivated by a lack of data about inflammation properties of O. selloi.
33892069	6	7	theme	300 mg/kg	826:834	arg1	doses					805:809	doses	805:809	doses of 30, 100 and 300 mg/kg	805:834	OSAE and OSEO were tested orally at doses of 30, 100 and 300 mg/kg at the carrageenan-induced pleurisy and paw edema, also mechanical hyperalgesia, in mice.
33892069	5	8	theme	antioxidant	600:610	arg1	MATERIALS					574:582	MATERIALS	574:582	MATERIALS	574:582	MATERIALS AND METHODS The antioxidant activity and total phenolic content were evaluated for samples, although chemical composition was obtained by U-HPLC-DAD-ESI-MS for OSAE and GC-MS for OSEO.
33892069	5	8	theme	antioxidant	600:610	arg1	activity					612:619	The antioxidant activity	596:619	The antioxidant activity	596:619	MATERIALS AND METHODS The antioxidant activity and total phenolic content were evaluated for samples, although chemical composition was obtained by U-HPLC-DAD-ESI-MS for OSAE and GC-MS for OSEO.
33892069	4	9	dep	AIM	370:372	arg1	investigated					398:409	investigated	398:409	investigated the chemical composition and anti-inflammatory activity, in mice models, of the aqueous extract (OSAE) and essential oil (OSEO) obtained from leaves of O. selloi	398:571	AIM OF THE STUDY This study investigated the chemical composition and anti-inflammatory activity, in mice models, of the aqueous extract (OSAE) and essential oil (OSEO) obtained from leaves of O. selloi.
33892069	4	10	dep	composition	424:434	arg1	the					411:413	the	411:413	the	411:413	AIM OF THE STUDY This study investigated the chemical composition and anti-inflammatory activity, in mice models, of the aqueous extract (OSAE) and essential oil (OSEO) obtained from leaves of O. selloi.
33892069	4	11	theme	STUDY	381:385	arg1	AIM					370:372	AIM	370:372	AIM OF THE STUDY This study investigated the chemical composition and anti-inflammatory activity, in mice models, of the aqueous extract (OSAE) and essential oil (OSEO) obtained from leaves of O. selloi.	370:572	AIM OF THE STUDY This study investigated the chemical composition and anti-inflammatory activity, in mice models, of the aqueous extract (OSAE) and essential oil (OSEO) obtained from leaves of O. selloi.
33892069	1	12	dep	RELEVANCE	146:154	arg1	population					160:169	The population	156:169	ETHNOPHARMACOLOGICAL RELEVANCE The population	125:169	ETHNOPHARMACOLOGICAL RELEVANCE The population uses the aqueous extract as tea from leaves of Ocimum selloi Benth.
33892069	7	13	dep	RESULTS	926:932	arg1	flavonoids					952:961	Four glycosylated flavonoids	934:961	Four glycosylated flavonoids	934:961	RESULTS Four glycosylated flavonoids and one organic acid were identified in OSAE, and nine substances in OSEO, the two majoritarian are E-anethole and methyl chavicol.
33892069	3	14	theme	data	321:324	arg1	lack					313:316	a lack	311:316	a lack of data about inflammation properties of O. selloi	311:367	This study is motivated by a lack of data about inflammation properties of O. selloi.
33892069	6	15	theme	100	818:820	arg1	doses					805:809	doses	805:809	doses of 30, 100 and 300 mg/kg	805:834	OSAE and OSEO were tested orally at doses of 30, 100 and 300 mg/kg at the carrageenan-induced pleurisy and paw edema, also mechanical hyperalgesia, in mice.
33892069	9	16	theme	300 mg/kg	1413:1421	arg1	dose					1405:1408	The dose	1401:1408	The dose of 300 mg/kg of OSEO	1401:1429	The dose of 300 mg/kg of OSEO reduced cold hyperalgesia 4 h after carrageenan.
33892069	10	17	theme	anti-inflammatory	1517:1533	arg1	potentials					1595:1604	the anti-inflammatory, anti-edematogenic, anti-hyperalgesic, and anti-nociceptive potentials	1513:1604	the anti-inflammatory, anti-edematogenic, anti-hyperalgesic, and anti-nociceptive potentials of both materials obtained from leaves of O. selloi, mainly OSAE	1513:1669	CONCLUSION The results evidenced the anti-inflammatory, anti-edematogenic, anti-hyperalgesic, and anti-nociceptive potentials of both materials obtained from leaves of O. selloi, mainly OSAE, supporting the popular use of this species.
33892069	1	18	theme	aqueous	180:186	arg1	extract					188:194	the aqueous extract	176:194	the aqueous extract as tea from leaves of Ocimum selloi Benth	176:236	ETHNOPHARMACOLOGICAL RELEVANCE The population uses the aqueous extract as tea from leaves of Ocimum selloi Benth.
33892069	4	19	theme	mice	471:474	arg1	models					476:481	mice models	471:481	mice models	471:481	AIM OF THE STUDY This study investigated the chemical composition and anti-inflammatory activity, in mice models, of the aqueous extract (OSAE) and essential oil (OSEO) obtained from leaves of O. selloi.
33892069	8	20	theme	Oral	1095:1098	arg1	treatments					1100:1109	Oral treatments	1095:1109	Oral treatments with OSAE and OSEO	1095:1128	Oral treatments with OSAE and OSEO significantly inhibited the carrageenan-induced pleurisy in female Swiss mice, besides OSAE and OSEO significantly prevented paw edema (after 1, 2, and 4 h), mechanical hyperalgesia (after 3 and 4 h), and cold hyperalgesia 3 h after carrageenan model in male Swiss mice.
33892069	5	21	theme	chemical	685:692	arg1	composition					694:704	chemical composition	685:704	chemical composition	685:704	MATERIALS AND METHODS The antioxidant activity and total phenolic content were evaluated for samples, although chemical composition was obtained by U-HPLC-DAD-ESI-MS for OSAE and GC-MS for OSEO.
33892069	6	22	theme	30	814:815	arg1	doses					805:809	doses	805:809	doses of 30, 100 and 300 mg/kg	805:834	OSAE and OSEO were tested orally at doses of 30, 100 and 300 mg/kg at the carrageenan-induced pleurisy and paw edema, also mechanical hyperalgesia, in mice.
33892069	9	23	theme	OSEO	1426:1429	arg1	300 mg/kg					1413:1421	300 mg/kg	1413:1421	300 mg/kg of OSEO	1413:1429	The dose of 300 mg/kg of OSEO reduced cold hyperalgesia 4 h after carrageenan.
33892069	8	24	theme	Swiss	1389:1393	arg1	mice					1395:1398	male Swiss mice	1384:1398	male Swiss mice	1384:1398	Oral treatments with OSAE and OSEO significantly inhibited the carrageenan-induced pleurisy in female Swiss mice, besides OSAE and OSEO significantly prevented paw edema (after 1, 2, and 4 h), mechanical hyperalgesia (after 3 and 4 h), and cold hyperalgesia 3 h after carrageenan model in male Swiss mice.
33892069	2	25	dep	pain	254:257	arg1	issues					276:281	issues	276:281	issues	276:281	(alfavaca) for pain and inflammation issues.
33892069	2	25	dep	pain	254:257	arg1	alfavaca					240:247	alfavaca	240:247	alfavaca	240:247	(alfavaca) for pain and inflammation issues.
33892069	0	26	theme	Anti-inflammatory	0:16	arg1	activity					18:25	Anti-inflammatory activity	0:25	Anti-inflammatory activity	0:25	Anti-inflammatory activity and chemical composition of aqueous extract and essential oil from leaves of Ocimum selloi Benth.
33892069	7	27	gly	glycosylated	939:950	arg1	flavonoids					952:961	Four glycosylated flavonoids	934:961	Four glycosylated flavonoids	934:961	RESULTS Four glycosylated flavonoids and one organic acid were identified in OSAE, and nine substances in OSEO, the two majoritarian are E-anethole and methyl chavicol.
33892069	4	28	from	activity	458:465	arg1	models					476:481	mice models	471:481	mice models	471:481	AIM OF THE STUDY This study investigated the chemical composition and anti-inflammatory activity, in mice models, of the aqueous extract (OSAE) and essential oil (OSEO) obtained from leaves of O. selloi.
33892069	0	29	theme	chemical	31:38	arg1	composition					40:50	chemical composition	31:50	chemical composition	31:50	Anti-inflammatory activity and chemical composition of aqueous extract and essential oil from leaves of Ocimum selloi Benth.
33892069	6	30	theme	mechanical	892:901	arg1	hyperalgesia					903:914	mechanical hyperalgesia	892:914	mechanical hyperalgesia	892:914	OSAE and OSEO were tested orally at doses of 30, 100 and 300 mg/kg at the carrageenan-induced pleurisy and paw edema, also mechanical hyperalgesia, in mice.
33892069	8	31	dep	inhibited	1144:1152	arg1	besides					1209:1215	besides	1209:1215	besides	1209:1215	Oral treatments with OSAE and OSEO significantly inhibited the carrageenan-induced pleurisy in female Swiss mice, besides OSAE and OSEO significantly prevented paw edema (after 1, 2, and 4 h), mechanical hyperalgesia (after 3 and 4 h), and cold hyperalgesia 3 h after carrageenan model in male Swiss mice.
33892069	9	32	theme	cold	1439:1442	arg1	4 h					1457:1459	cold hyperalgesia 4 h	1439:1459	cold hyperalgesia 4 h	1439:1459	The dose of 300 mg/kg of OSEO reduced cold hyperalgesia 4 h after carrageenan.
33892069	8	33	theme	Swiss	1197:1201	arg1	mice					1203:1206	female Swiss mice	1190:1206	female Swiss mice	1190:1206	Oral treatments with OSAE and OSEO significantly inhibited the carrageenan-induced pleurisy in female Swiss mice, besides OSAE and OSEO significantly prevented paw edema (after 1, 2, and 4 h), mechanical hyperalgesia (after 3 and 4 h), and cold hyperalgesia 3 h after carrageenan model in male Swiss mice.
33892069	3	34	theme	O.	359:360	arg1	properties					345:354	inflammation properties	332:354	inflammation properties of O. selloi	332:367	This study is motivated by a lack of data about inflammation properties of O. selloi.
33892069	4	35	theme	oil	528:530	arg1	composition					424:434	chemical composition	415:434	chemical composition	415:434	AIM OF THE STUDY This study investigated the chemical composition and anti-inflammatory activity, in mice models, of the aqueous extract (OSAE) and essential oil (OSEO) obtained from leaves of O. selloi.
33892069	4	35	theme	oil	528:530	arg1	activity					458:465	anti-inflammatory activity	440:465	anti-inflammatory activity	440:465	AIM OF THE STUDY This study investigated the chemical composition and anti-inflammatory activity, in mice models, of the aqueous extract (OSAE) and essential oil (OSEO) obtained from leaves of O. selloi.
33892069	9	36	theme	hyperalgesia	1444:1455	arg1	4 h					1457:1459	cold hyperalgesia 4 h	1439:1459	cold hyperalgesia 4 h	1439:1459	The dose of 300 mg/kg of OSEO reduced cold hyperalgesia 4 h after carrageenan.
33892069	8	37	theme	female	1190:1195	arg1	mice					1203:1206	female Swiss mice	1190:1206	female Swiss mice	1190:1206	Oral treatments with OSAE and OSEO significantly inhibited the carrageenan-induced pleurisy in female Swiss mice, besides OSAE and OSEO significantly prevented paw edema (after 1, 2, and 4 h), mechanical hyperalgesia (after 3 and 4 h), and cold hyperalgesia 3 h after carrageenan model in male Swiss mice.
33892069	5	38	dep	MATERIALS	574:582	arg1	MATERIALS					574:582	MATERIALS	574:582	MATERIALS	574:582	MATERIALS AND METHODS The antioxidant activity and total phenolic content were evaluated for samples, although chemical composition was obtained by U-HPLC-DAD-ESI-MS for OSAE and GC-MS for OSEO.
33892069	5	38	dep	MATERIALS	574:582	arg1	content					640:646	total phenolic content	625:646	total phenolic content	625:646	MATERIALS AND METHODS The antioxidant activity and total phenolic content were evaluated for samples, although chemical composition was obtained by U-HPLC-DAD-ESI-MS for OSAE and GC-MS for OSEO.
33892069	5	38	dep	MATERIALS	574:582	arg1	activity					612:619	The antioxidant activity	596:619	The antioxidant activity	596:619	MATERIALS AND METHODS The antioxidant activity and total phenolic content were evaluated for samples, although chemical composition was obtained by U-HPLC-DAD-ESI-MS for OSAE and GC-MS for OSEO.
33892069	4	39	theme	essential	518:526	arg1	OSEO					533:536	OSEO	533:536	OSEO	533:536	AIM OF THE STUDY This study investigated the chemical composition and anti-inflammatory activity, in mice models, of the aqueous extract (OSAE) and essential oil (OSEO) obtained from leaves of O. selloi.
33892069	4	39	theme	essential	518:526	arg1	oil					528:530	essential oil	518:530	essential oil (OSEO)	518:537	AIM OF THE STUDY This study investigated the chemical composition and anti-inflammatory activity, in mice models, of the aqueous extract (OSAE) and essential oil (OSEO) obtained from leaves of O. selloi.
33892069	4	40	from	composition	424:434	arg1	models					476:481	mice models	471:481	mice models	471:481	AIM OF THE STUDY This study investigated the chemical composition and anti-inflammatory activity, in mice models, of the aqueous extract (OSAE) and essential oil (OSEO) obtained from leaves of O. selloi.
33892069	4	41	theme	chemical	415:422	arg1	composition					424:434	chemical composition	415:434	chemical composition	415:434	AIM OF THE STUDY This study investigated the chemical composition and anti-inflammatory activity, in mice models, of the aqueous extract (OSAE) and essential oil (OSEO) obtained from leaves of O. selloi.
33892069	8	42	dep	edema	1259:1263	arg1	2					1275:1275	2	1275:1275	2	1275:1275	Oral treatments with OSAE and OSEO significantly inhibited the carrageenan-induced pleurisy in female Swiss mice, besides OSAE and OSEO significantly prevented paw edema (after 1, 2, and 4 h), mechanical hyperalgesia (after 3 and 4 h), and cold hyperalgesia 3 h after carrageenan model in male Swiss mice.
33892069	8	42	dep	edema	1259:1263	arg1	4 h					1282:1284	4 h	1282:1284	4 h	1282:1284	Oral treatments with OSAE and OSEO significantly inhibited the carrageenan-induced pleurisy in female Swiss mice, besides OSAE and OSEO significantly prevented paw edema (after 1, 2, and 4 h), mechanical hyperalgesia (after 3 and 4 h), and cold hyperalgesia 3 h after carrageenan model in male Swiss mice.
33892069	10	43	theme	anti-hyperalgesic	1555:1571	arg1	potentials					1595:1604	the anti-inflammatory, anti-edematogenic, anti-hyperalgesic, and anti-nociceptive potentials	1513:1604	the anti-inflammatory, anti-edematogenic, anti-hyperalgesic, and anti-nociceptive potentials of both materials obtained from leaves of O. selloi, mainly OSAE	1513:1669	CONCLUSION The results evidenced the anti-inflammatory, anti-edematogenic, anti-hyperalgesic, and anti-nociceptive potentials of both materials obtained from leaves of O. selloi, mainly OSAE, supporting the popular use of this species.
33892069	8	44	theme	carrageenan-induced	1158:1176	arg1	pleurisy					1178:1185	the carrageenan-induced pleurisy	1154:1185	the carrageenan-induced pleurisy in female Swiss mice	1154:1206	Oral treatments with OSAE and OSEO significantly inhibited the carrageenan-induced pleurisy in female Swiss mice, besides OSAE and OSEO significantly prevented paw edema (after 1, 2, and 4 h), mechanical hyperalgesia (after 3 and 4 h), and cold hyperalgesia 3 h after carrageenan model in male Swiss mice.
33892069	4	45	theme	extract	499:505	arg1	composition					424:434	chemical composition	415:434	chemical composition	415:434	AIM OF THE STUDY This study investigated the chemical composition and anti-inflammatory activity, in mice models, of the aqueous extract (OSAE) and essential oil (OSEO) obtained from leaves of O. selloi.
33892069	4	45	theme	extract	499:505	arg1	activity					458:465	anti-inflammatory activity	440:465	anti-inflammatory activity	440:465	AIM OF THE STUDY This study investigated the chemical composition and anti-inflammatory activity, in mice models, of the aqueous extract (OSAE) and essential oil (OSEO) obtained from leaves of O. selloi.
33892069	10	46	theme	anti-edematogenic	1536:1552	arg1	potentials					1595:1604	the anti-inflammatory, anti-edematogenic, anti-hyperalgesic, and anti-nociceptive potentials	1513:1604	the anti-inflammatory, anti-edematogenic, anti-hyperalgesic, and anti-nociceptive potentials of both materials obtained from leaves of O. selloi, mainly OSAE	1513:1669	CONCLUSION The results evidenced the anti-inflammatory, anti-edematogenic, anti-hyperalgesic, and anti-nociceptive potentials of both materials obtained from leaves of O. selloi, mainly OSAE, supporting the popular use of this species.
33892069	4	47	theme	anti-inflammatory	440:456	arg1	activity					458:465	anti-inflammatory activity	440:465	anti-inflammatory activity	440:465	AIM OF THE STUDY This study investigated the chemical composition and anti-inflammatory activity, in mice models, of the aqueous extract (OSAE) and essential oil (OSEO) obtained from leaves of O. selloi.
33892069	8	48	from	model	1375:1379	arg1	mice					1395:1398	male Swiss mice	1384:1398	male Swiss mice	1384:1398	Oral treatments with OSAE and OSEO significantly inhibited the carrageenan-induced pleurisy in female Swiss mice, besides OSAE and OSEO significantly prevented paw edema (after 1, 2, and 4 h), mechanical hyperalgesia (after 3 and 4 h), and cold hyperalgesia 3 h after carrageenan model in male Swiss mice.
33892069	7	49	theme	glycosylated	939:950	arg1	flavonoids					952:961	Four glycosylated flavonoids	934:961	Four glycosylated flavonoids	934:961	RESULTS Four glycosylated flavonoids and one organic acid were identified in OSAE, and nine substances in OSEO, the two majoritarian are E-anethole and methyl chavicol.
33892069	0	50	theme	extract	63:69	arg1	activity					18:25	Anti-inflammatory activity	0:25	Anti-inflammatory activity	0:25	Anti-inflammatory activity and chemical composition of aqueous extract and essential oil from leaves of Ocimum selloi Benth.
33892069	0	50	theme	extract	63:69	arg1	composition					40:50	chemical composition	31:50	chemical composition	31:50	Anti-inflammatory activity and chemical composition of aqueous extract and essential oil from leaves of Ocimum selloi Benth.
33892069	4	51	theme	aqueous	491:497	arg1	OSAE					508:511	OSAE	508:511	OSAE	508:511	AIM OF THE STUDY This study investigated the chemical composition and anti-inflammatory activity, in mice models, of the aqueous extract (OSAE) and essential oil (OSEO) obtained from leaves of O. selloi.
33892069	4	51	theme	aqueous	491:497	arg1	extract					499:505	the aqueous extract	487:505	the aqueous extract (OSAE)	487:512	AIM OF THE STUDY This study investigated the chemical composition and anti-inflammatory activity, in mice models, of the aqueous extract (OSAE) and essential oil (OSEO) obtained from leaves of O. selloi.
33892069	7	52	theme	organic	971:977	arg1	acid					979:982	one organic acid	967:982	one organic acid	967:982	RESULTS Four glycosylated flavonoids and one organic acid were identified in OSAE, and nine substances in OSEO, the two majoritarian are E-anethole and methyl chavicol.
33892069	10	53	theme	popular	1687:1693	arg1	use					1695:1697	the popular use	1683:1697	the popular use of this species	1683:1713	CONCLUSION The results evidenced the anti-inflammatory, anti-edematogenic, anti-hyperalgesic, and anti-nociceptive potentials of both materials obtained from leaves of O. selloi, mainly OSAE, supporting the popular use of this species.
33892069	8	54	from	pleurisy	1178:1185	arg1	mice					1203:1206	female Swiss mice	1190:1206	female Swiss mice	1190:1206	Oral treatments with OSAE and OSEO significantly inhibited the carrageenan-induced pleurisy in female Swiss mice, besides OSAE and OSEO significantly prevented paw edema (after 1, 2, and 4 h), mechanical hyperalgesia (after 3 and 4 h), and cold hyperalgesia 3 h after carrageenan model in male Swiss mice.
33892069	0	55	theme	aqueous	55:61	arg1	extract					63:69	aqueous extract	55:69	aqueous extract	55:69	Anti-inflammatory activity and chemical composition of aqueous extract and essential oil from leaves of Ocimum selloi Benth.
33892069	5	56	theme	total	625:629	arg1	MATERIALS					574:582	MATERIALS	574:582	MATERIALS	574:582	MATERIALS AND METHODS The antioxidant activity and total phenolic content were evaluated for samples, although chemical composition was obtained by U-HPLC-DAD-ESI-MS for OSAE and GC-MS for OSEO.
33892069	5	56	theme	total	625:629	arg1	content					640:646	total phenolic content	625:646	total phenolic content	625:646	MATERIALS AND METHODS The antioxidant activity and total phenolic content were evaluated for samples, although chemical composition was obtained by U-HPLC-DAD-ESI-MS for OSAE and GC-MS for OSEO.
33892069	8	57	theme	hyperalgesia	1340:1351	arg1	3 h					1353:1355	cold hyperalgesia 3 h	1335:1355	cold hyperalgesia 3 h	1335:1355	Oral treatments with OSAE and OSEO significantly inhibited the carrageenan-induced pleurisy in female Swiss mice, besides OSAE and OSEO significantly prevented paw edema (after 1, 2, and 4 h), mechanical hyperalgesia (after 3 and 4 h), and cold hyperalgesia 3 h after carrageenan model in male Swiss mice.
33892069	8	58	theme	mechanical	1288:1297	arg1	hyperalgesia					1299:1310	mechanical hyperalgesia	1288:1310	mechanical hyperalgesia (after 3 and 4 h)	1288:1328	Oral treatments with OSAE and OSEO significantly inhibited the carrageenan-induced pleurisy in female Swiss mice, besides OSAE and OSEO significantly prevented paw edema (after 1, 2, and 4 h), mechanical hyperalgesia (after 3 and 4 h), and cold hyperalgesia 3 h after carrageenan model in male Swiss mice.
33892069	8	59	with	treatments	1100:1109	arg1	OSEO					1125:1128	OSEO	1125:1128	OSEO	1125:1128	Oral treatments with OSAE and OSEO significantly inhibited the carrageenan-induced pleurisy in female Swiss mice, besides OSAE and OSEO significantly prevented paw edema (after 1, 2, and 4 h), mechanical hyperalgesia (after 3 and 4 h), and cold hyperalgesia 3 h after carrageenan model in male Swiss mice.
33892069	8	59	with	treatments	1100:1109	arg1	OSAE					1116:1119	OSAE	1116:1119	OSAE	1116:1119	Oral treatments with OSAE and OSEO significantly inhibited the carrageenan-induced pleurisy in female Swiss mice, besides OSAE and OSEO significantly prevented paw edema (after 1, 2, and 4 h), mechanical hyperalgesia (after 3 and 4 h), and cold hyperalgesia 3 h after carrageenan model in male Swiss mice.
33892069	5	60	theme	phenolic	631:638	arg1	MATERIALS					574:582	MATERIALS	574:582	MATERIALS	574:582	MATERIALS AND METHODS The antioxidant activity and total phenolic content were evaluated for samples, although chemical composition was obtained by U-HPLC-DAD-ESI-MS for OSAE and GC-MS for OSEO.
33892069	5	60	theme	phenolic	631:638	arg1	content					640:646	total phenolic content	625:646	total phenolic content	625:646	MATERIALS AND METHODS The antioxidant activity and total phenolic content were evaluated for samples, although chemical composition was obtained by U-HPLC-DAD-ESI-MS for OSAE and GC-MS for OSEO.
33892069	8	61	theme	cold	1335:1338	arg1	3 h					1353:1355	cold hyperalgesia 3 h	1335:1355	cold hyperalgesia 3 h	1335:1355	Oral treatments with OSAE and OSEO significantly inhibited the carrageenan-induced pleurisy in female Swiss mice, besides OSAE and OSEO significantly prevented paw edema (after 1, 2, and 4 h), mechanical hyperalgesia (after 3 and 4 h), and cold hyperalgesia 3 h after carrageenan model in male Swiss mice.
33892069	1	62	from	leaves	208:213	arg1	extract					188:194	the aqueous extract	176:194	the aqueous extract as tea from leaves of Ocimum selloi Benth	176:236	ETHNOPHARMACOLOGICAL RELEVANCE The population uses the aqueous extract as tea from leaves of Ocimum selloi Benth.
33892069	1	63	theme	ETHNOPHARMACOLOGICAL	125:144	arg1	RELEVANCE					146:154	ETHNOPHARMACOLOGICAL RELEVANCE The population	125:169	ETHNOPHARMACOLOGICAL RELEVANCE The population	125:169	ETHNOPHARMACOLOGICAL RELEVANCE The population uses the aqueous extract as tea from leaves of Ocimum selloi Benth.
33892069	7	64	from	substances	1018:1027	arg1	OSEO					1032:1035	OSEO	1032:1035	OSEO	1032:1035	RESULTS Four glycosylated flavonoids and one organic acid were identified in OSAE, and nine substances in OSEO, the two majoritarian are E-anethole and methyl chavicol.
33892069	7	64	from	substances	1018:1027	arg1	majoritarian					1046:1057	the two majoritarian	1038:1057	the two majoritarian	1038:1057	RESULTS Four glycosylated flavonoids and one organic acid were identified in OSAE, and nine substances in OSEO, the two majoritarian are E-anethole and methyl chavicol.
33892069	10	65	theme	anti-nociceptive	1578:1593	arg1	potentials					1595:1604	the anti-inflammatory, anti-edematogenic, anti-hyperalgesic, and anti-nociceptive potentials	1513:1604	the anti-inflammatory, anti-edematogenic, anti-hyperalgesic, and anti-nociceptive potentials of both materials obtained from leaves of O. selloi, mainly OSAE	1513:1669	CONCLUSION The results evidenced the anti-inflammatory, anti-edematogenic, anti-hyperalgesic, and anti-nociceptive potentials of both materials obtained from leaves of O. selloi, mainly OSAE, supporting the popular use of this species.
33892069	0	66	theme	essential	75:83	arg1	oil					85:87	essential oil	75:87	essential oil	75:87	Anti-inflammatory activity and chemical composition of aqueous extract and essential oil from leaves of Ocimum selloi Benth.
33892069	0	67	from	leaves	94:99	arg1	activity					18:25	Anti-inflammatory activity	0:25	Anti-inflammatory activity	0:25	Anti-inflammatory activity and chemical composition of aqueous extract and essential oil from leaves of Ocimum selloi Benth.
33892069	0	67	from	leaves	94:99	arg1	composition					40:50	chemical composition	31:50	chemical composition	31:50	Anti-inflammatory activity and chemical composition of aqueous extract and essential oil from leaves of Ocimum selloi Benth.
33892069	10	68	theme	species	1707:1713	arg1	use					1695:1697	the popular use	1683:1697	the popular use of this species	1683:1713	CONCLUSION The results evidenced the anti-inflammatory, anti-edematogenic, anti-hyperalgesic, and anti-nociceptive potentials of both materials obtained from leaves of O. selloi, mainly OSAE, supporting the popular use of this species.
33892069	4	69	theme	O.	563:564	arg1	selloi					566:571	O. selloi	563:571	O. selloi	563:571	AIM OF THE STUDY This study investigated the chemical composition and anti-inflammatory activity, in mice models, of the aqueous extract (OSAE) and essential oil (OSEO) obtained from leaves of O. selloi.
33892069	6	70	theme	carrageenan-induced	843:861	arg1	pleurisy					863:870	the carrageenan-induced pleurisy	839:870	the carrageenan-induced pleurisy	839:870	OSAE and OSEO were tested orally at doses of 30, 100 and 300 mg/kg at the carrageenan-induced pleurisy and paw edema, also mechanical hyperalgesia, in mice.
33892069	7	71	theme	methyl	1078:1083	arg1	chavicol					1085:1092	methyl chavicol	1078:1092	methyl chavicol	1078:1092	RESULTS Four glycosylated flavonoids and one organic acid were identified in OSAE, and nine substances in OSEO, the two majoritarian are E-anethole and methyl chavicol.
33892069	6	72	theme	paw	876:878	arg1	edema					880:884	paw edema	876:884	paw edema	876:884	OSAE and OSEO were tested orally at doses of 30, 100 and 300 mg/kg at the carrageenan-induced pleurisy and paw edema, also mechanical hyperalgesia, in mice.
32770506	0	0	theme	Proteins	90:97	arg1	Isolation					11:19	Lipid Raft Isolation	0:19	Lipid Raft Isolation by Sucrose Gradient Centrifugation	0:54	Lipid Raft Isolation by Sucrose Gradient Centrifugation and Visualization of Raft-Located Proteins by Fluorescence Microscopy: The Use of Combined Techniques to Assess Fas/CD95 Location in Rafts During Apoptosis Triggering.
32770506	0	0	theme	Proteins	90:97	arg1	Visualization					60:72	Visualization	60:72	Visualization of Raft-Located Proteins by Fluorescence Microscopy	60:124	Lipid Raft Isolation by Sucrose Gradient Centrifugation and Visualization of Raft-Located Proteins by Fluorescence Microscopy: The Use of Combined Techniques to Assess Fas/CD95 Location in Rafts During Apoptosis Triggering.
32770506	5	1	theme	rafts	1154:1158	arg1	coclustering					1104:1115	the coclustering	1100:1115	the coclustering of Fas/CD95 death receptor and lipid rafts	1100:1158	In addition, we also provide a detailed protocol for the visualization of the coclustering of Fas/CD95 death receptor and lipid rafts, as assessed by using anti-Fas/CD95 antibodies and fluorescent dye-conjugated cholera toxin B subunit that binds to ganglioside GM1, a main component of lipid rafts, by immunofluorescence and confocal microscopy.
32770506	4	2	theme	subsequent	886:895	arg1	centrifugation					914:927	subsequent sucrose gradient centrifugation	886:927	subsequent sucrose gradient centrifugation	886:927	A detailed protocol is provided for the isolation of lipid rafts, by taking advantage of their resistance to Triton X-100 solubilization at 4 °C, followed by subsequent sucrose gradient centrifugation and analysis of the protein composition of the different gradient fractions by Western blotting.
32770506	4	3	theme	detailed	730:737	arg1	protocol					739:746	A detailed protocol	728:746	A detailed protocol	728:746	A detailed protocol is provided for the isolation of lipid rafts, by taking advantage of their resistance to Triton X-100 solubilization at 4 °C, followed by subsequent sucrose gradient centrifugation and analysis of the protein composition of the different gradient fractions by Western blotting.
32770506	5	4	theme	cholera	1238:1244	arg1	toxin					1246:1250	fluorescent dye-conjugated cholera toxin	1211:1250	fluorescent dye-conjugated cholera toxin B subunit that binds to ganglioside GM1, a main component of lipid rafts, by immunofluorescence and confocal microscopy	1211:1370	In addition, we also provide a detailed protocol for the visualization of the coclustering of Fas/CD95 death receptor and lipid rafts, as assessed by using anti-Fas/CD95 antibodies and fluorescent dye-conjugated cholera toxin B subunit that binds to ganglioside GM1, a main component of lipid rafts, by immunofluorescence and confocal microscopy.
32770506	0	5	theme	Raft-Located	77:88	arg1	Proteins					90:97	Raft-Located Proteins	77:97	Raft-Located Proteins	77:97	Lipid Raft Isolation by Sucrose Gradient Centrifugation and Visualization of Raft-Located Proteins by Fluorescence Microscopy: The Use of Combined Techniques to Assess Fas/CD95 Location in Rafts During Apoptosis Triggering.
32770506	5	6	theme	toxin	1246:1250	arg1	subunit					1254:1260	fluorescent dye-conjugated cholera toxin B subunit	1211:1260	fluorescent dye-conjugated cholera toxin B subunit that binds to ganglioside GM1, a main component of lipid rafts, by immunofluorescence and confocal microscopy	1211:1370	In addition, we also provide a detailed protocol for the visualization of the coclustering of Fas/CD95 death receptor and lipid rafts, as assessed by using anti-Fas/CD95 antibodies and fluorescent dye-conjugated cholera toxin B subunit that binds to ganglioside GM1, a main component of lipid rafts, by immunofluorescence and confocal microscopy.
32770506	0	7	theme	Fluorescence	102:113	arg1	Microscopy					115:124	Fluorescence Microscopy	102:124	Fluorescence Microscopy	102:124	Lipid Raft Isolation by Sucrose Gradient Centrifugation and Visualization of Raft-Located Proteins by Fluorescence Microscopy: The Use of Combined Techniques to Assess Fas/CD95 Location in Rafts During Apoptosis Triggering.
32770506	5	8	theme	lipid	1148:1152	arg1	rafts					1154:1158	lipid rafts	1148:1158	lipid rafts	1148:1158	In addition, we also provide a detailed protocol for the visualization of the coclustering of Fas/CD95 death receptor and lipid rafts, as assessed by using anti-Fas/CD95 antibodies and fluorescent dye-conjugated cholera toxin B subunit that binds to ganglioside GM1, a main component of lipid rafts, by immunofluorescence and confocal microscopy.
32770506	2	9	theme	raft	510:513	arg1	domains					515:521	these raft domains	504:521	these raft domains	504:521	Death receptors and downstream signaling molecules have been reported to be recruited into these raft domains during the triggering of apoptosis.
32770506	3	10	attach	presence	607:614	arg1	rafts					637:641	lipid rafts	631:641	lipid rafts	631:641	Here, we provide two protocols that support the presence of Fas/CD95 in lipid rafts during apoptosis, involving lipid raft isolation and confocal microscopy techniques.
32770506	3	10	attach	presence	607:614	arg2	Fas/CD95					619:626	Fas/CD95	619:626	Fas/CD95	619:626	Here, we provide two protocols that support the presence of Fas/CD95 in lipid rafts during apoptosis, involving lipid raft isolation and confocal microscopy techniques.
32770506	5	11	theme	B	1252:1252	arg1	subunit					1254:1260	fluorescent dye-conjugated cholera toxin B subunit	1211:1260	fluorescent dye-conjugated cholera toxin B subunit that binds to ganglioside GM1, a main component of lipid rafts, by immunofluorescence and confocal microscopy	1211:1370	In addition, we also provide a detailed protocol for the visualization of the coclustering of Fas/CD95 death receptor and lipid rafts, as assessed by using anti-Fas/CD95 antibodies and fluorescent dye-conjugated cholera toxin B subunit that binds to ganglioside GM1, a main component of lipid rafts, by immunofluorescence and confocal microscopy.
32770506	5	12	theme	Fas/CD95	1120:1127	arg1	receptor					1135:1142	Fas/CD95 death receptor	1120:1142	Fas/CD95 death receptor	1120:1142	In addition, we also provide a detailed protocol for the visualization of the coclustering of Fas/CD95 death receptor and lipid rafts, as assessed by using anti-Fas/CD95 antibodies and fluorescent dye-conjugated cholera toxin B subunit that binds to ganglioside GM1, a main component of lipid rafts, by immunofluorescence and confocal microscopy.
32770506	4	13	theme	sucrose	897:903	arg1	centrifugation					914:927	subsequent sucrose gradient centrifugation	886:927	subsequent sucrose gradient centrifugation	886:927	A detailed protocol is provided for the isolation of lipid rafts, by taking advantage of their resistance to Triton X-100 solubilization at 4 °C, followed by subsequent sucrose gradient centrifugation and analysis of the protein composition of the different gradient fractions by Western blotting.
32770506	4	14	theme	rafts	787:791	arg1	isolation					768:776	the isolation	764:776	the isolation of lipid rafts	764:791	A detailed protocol is provided for the isolation of lipid rafts, by taking advantage of their resistance to Triton X-100 solubilization at 4 °C, followed by subsequent sucrose gradient centrifugation and analysis of the protein composition of the different gradient fractions by Western blotting.
32770506	5	15	theme	confocal	1352:1359	arg1	microscopy					1361:1370	confocal microscopy	1352:1370	confocal microscopy	1352:1370	In addition, we also provide a detailed protocol for the visualization of the coclustering of Fas/CD95 death receptor and lipid rafts, as assessed by using anti-Fas/CD95 antibodies and fluorescent dye-conjugated cholera toxin B subunit that binds to ganglioside GM1, a main component of lipid rafts, by immunofluorescence and confocal microscopy.
32770506	2	16	theme	apoptosis	548:556	arg1	triggering					534:543	the triggering	530:543	the triggering of apoptosis	530:556	Death receptors and downstream signaling molecules have been reported to be recruited into these raft domains during the triggering of apoptosis.
32770506	5	17	theme	death	1129:1133	arg1	receptor					1135:1142	Fas/CD95 death receptor	1120:1142	Fas/CD95 death receptor	1120:1142	In addition, we also provide a detailed protocol for the visualization of the coclustering of Fas/CD95 death receptor and lipid rafts, as assessed by using anti-Fas/CD95 antibodies and fluorescent dye-conjugated cholera toxin B subunit that binds to ganglioside GM1, a main component of lipid rafts, by immunofluorescence and confocal microscopy.
32770506	4	18	theme	gradient	986:993	arg1	fractions					995:1003	the different gradient fractions	972:1003	the different gradient fractions	972:1003	A detailed protocol is provided for the isolation of lipid rafts, by taking advantage of their resistance to Triton X-100 solubilization at 4 °C, followed by subsequent sucrose gradient centrifugation and analysis of the protein composition of the different gradient fractions by Western blotting.
32770506	1	19	theme	signaling	393:401	arg1	pathways					403:410	signaling pathways	393:410	signaling pathways	393:410	Lipid rafts are heterogeneous membrane domains enriched in cholesterol, sphingolipids, and gangliosides that serve as sorting platforms to compartmentalize and modulate signaling pathways.
32770506	0	20	theme	Apoptosis	202:210	arg1	Triggering					212:221	Apoptosis Triggering	202:221	Apoptosis Triggering	202:221	Lipid Raft Isolation by Sucrose Gradient Centrifugation and Visualization of Raft-Located Proteins by Fluorescence Microscopy: The Use of Combined Techniques to Assess Fas/CD95 Location in Rafts During Apoptosis Triggering.
32770506	4	21	theme	fractions	995:1003	arg1	composition					957:967	the protein composition	945:967	the protein composition of the different gradient fractions	945:1003	A detailed protocol is provided for the isolation of lipid rafts, by taking advantage of their resistance to Triton X-100 solubilization at 4 °C, followed by subsequent sucrose gradient centrifugation and analysis of the protein composition of the different gradient fractions by Western blotting.
32770506	0	22	theme	Raft	6:9	arg1	Isolation					11:19	Lipid Raft Isolation	0:19	Lipid Raft Isolation by Sucrose Gradient Centrifugation	0:54	Lipid Raft Isolation by Sucrose Gradient Centrifugation and Visualization of Raft-Located Proteins by Fluorescence Microscopy: The Use of Combined Techniques to Assess Fas/CD95 Location in Rafts During Apoptosis Triggering.
32770506	5	23	theme	dye-conjugated	1223:1236	arg1	toxin					1246:1250	fluorescent dye-conjugated cholera toxin	1211:1250	fluorescent dye-conjugated cholera toxin B subunit that binds to ganglioside GM1, a main component of lipid rafts, by immunofluorescence and confocal microscopy	1211:1370	In addition, we also provide a detailed protocol for the visualization of the coclustering of Fas/CD95 death receptor and lipid rafts, as assessed by using anti-Fas/CD95 antibodies and fluorescent dye-conjugated cholera toxin B subunit that binds to ganglioside GM1, a main component of lipid rafts, by immunofluorescence and confocal microscopy.
32770506	2	24	theme	Death	413:417	arg1	receptors					419:427	Death receptors	413:427	Death receptors	413:427	Death receptors and downstream signaling molecules have been reported to be recruited into these raft domains during the triggering of apoptosis.
32770506	0	25	theme	Lipid	0:4	arg1	Isolation					11:19	Lipid Raft Isolation	0:19	Lipid Raft Isolation by Sucrose Gradient Centrifugation	0:54	Lipid Raft Isolation by Sucrose Gradient Centrifugation and Visualization of Raft-Located Proteins by Fluorescence Microscopy: The Use of Combined Techniques to Assess Fas/CD95 Location in Rafts During Apoptosis Triggering.
32770506	6	26	theme	lipid	1470:1474	arg1	rafts					1476:1480	lipid rafts	1470:1480	lipid rafts	1470:1480	These protocols can be extended to any protein of interest to be analyzed for its association to lipid rafts.
32770506	3	27	from	presence	607:614	arg1	rafts					637:641	lipid rafts	631:641	lipid rafts	631:641	Here, we provide two protocols that support the presence of Fas/CD95 in lipid rafts during apoptosis, involving lipid raft isolation and confocal microscopy techniques.
32770506	4	28	theme	resistance	823:832	arg1	advantage					804:812	advantage	804:812	advantage of their resistance to Triton X-100 solubilization	804:863	A detailed protocol is provided for the isolation of lipid rafts, by taking advantage of their resistance to Triton X-100 solubilization at 4 °C, followed by subsequent sucrose gradient centrifugation and analysis of the protein composition of the different gradient fractions by Western blotting.
32770506	4	29	theme	protein	949:955	arg1	composition					957:967	the protein composition	945:967	the protein composition of the different gradient fractions	945:1003	A detailed protocol is provided for the isolation of lipid rafts, by taking advantage of their resistance to Triton X-100 solubilization at 4 °C, followed by subsequent sucrose gradient centrifugation and analysis of the protein composition of the different gradient fractions by Western blotting.
32770506	5	30	theme	detailed	1057:1064	arg1	protocol					1066:1073	a detailed protocol	1055:1073	a detailed protocol for the visualization of the coclustering of Fas/CD95 death receptor and lipid rafts	1055:1158	In addition, we also provide a detailed protocol for the visualization of the coclustering of Fas/CD95 death receptor and lipid rafts, as assessed by using anti-Fas/CD95 antibodies and fluorescent dye-conjugated cholera toxin B subunit that binds to ganglioside GM1, a main component of lipid rafts, by immunofluorescence and confocal microscopy.
32770506	4	31	theme	lipid	781:785	arg1	rafts					787:791	lipid rafts	781:791	lipid rafts	781:791	A detailed protocol is provided for the isolation of lipid rafts, by taking advantage of their resistance to Triton X-100 solubilization at 4 °C, followed by subsequent sucrose gradient centrifugation and analysis of the protein composition of the different gradient fractions by Western blotting.
32770506	4	32	theme	Triton	837:842	arg1	solubilization					850:863	Triton X-100 solubilization	837:863	Triton X-100 solubilization	837:863	A detailed protocol is provided for the isolation of lipid rafts, by taking advantage of their resistance to Triton X-100 solubilization at 4 °C, followed by subsequent sucrose gradient centrifugation and analysis of the protein composition of the different gradient fractions by Western blotting.
32770506	5	33	theme	fluorescent	1211:1221	arg1	toxin					1246:1250	fluorescent dye-conjugated cholera toxin	1211:1250	fluorescent dye-conjugated cholera toxin B subunit that binds to ganglioside GM1, a main component of lipid rafts, by immunofluorescence and confocal microscopy	1211:1370	In addition, we also provide a detailed protocol for the visualization of the coclustering of Fas/CD95 death receptor and lipid rafts, as assessed by using anti-Fas/CD95 antibodies and fluorescent dye-conjugated cholera toxin B subunit that binds to ganglioside GM1, a main component of lipid rafts, by immunofluorescence and confocal microscopy.
32770506	3	34	theme	lipid	671:675	arg1	isolation					682:690	lipid raft isolation	671:690	lipid raft isolation	671:690	Here, we provide two protocols that support the presence of Fas/CD95 in lipid rafts during apoptosis, involving lipid raft isolation and confocal microscopy techniques.
32770506	5	35	theme	main	1295:1298	arg1	GM1					1288:1290	ganglioside GM1	1276:1290	ganglioside GM1	1276:1290	In addition, we also provide a detailed protocol for the visualization of the coclustering of Fas/CD95 death receptor and lipid rafts, as assessed by using anti-Fas/CD95 antibodies and fluorescent dye-conjugated cholera toxin B subunit that binds to ganglioside GM1, a main component of lipid rafts, by immunofluorescence and confocal microscopy.
32770506	5	35	theme	main	1295:1298	arg1	component					1300:1308	a main component	1293:1308	a main component of lipid rafts	1293:1323	In addition, we also provide a detailed protocol for the visualization of the coclustering of Fas/CD95 death receptor and lipid rafts, as assessed by using anti-Fas/CD95 antibodies and fluorescent dye-conjugated cholera toxin B subunit that binds to ganglioside GM1, a main component of lipid rafts, by immunofluorescence and confocal microscopy.
32770506	0	36	theme	Gradient	32:39	arg1	Centrifugation					41:54	Sucrose Gradient Centrifugation	24:54	Sucrose Gradient Centrifugation	24:54	Lipid Raft Isolation by Sucrose Gradient Centrifugation and Visualization of Raft-Located Proteins by Fluorescence Microscopy: The Use of Combined Techniques to Assess Fas/CD95 Location in Rafts During Apoptosis Triggering.
32770506	0	37	from	Location	177:184	arg1	Rafts					189:193	Rafts	189:193	Rafts	189:193	Lipid Raft Isolation by Sucrose Gradient Centrifugation and Visualization of Raft-Located Proteins by Fluorescence Microscopy: The Use of Combined Techniques to Assess Fas/CD95 Location in Rafts During Apoptosis Triggering.
32770506	0	38	theme	Combined	138:145	arg1	Techniques					147:156	Combined Techniques	138:156	Combined Techniques	138:156	Lipid Raft Isolation by Sucrose Gradient Centrifugation and Visualization of Raft-Located Proteins by Fluorescence Microscopy: The Use of Combined Techniques to Assess Fas/CD95 Location in Rafts During Apoptosis Triggering.
32770506	3	39	theme	raft	677:680	arg1	isolation					682:690	lipid raft isolation	671:690	lipid raft isolation	671:690	Here, we provide two protocols that support the presence of Fas/CD95 in lipid rafts during apoptosis, involving lipid raft isolation and confocal microscopy techniques.
32770506	3	40	theme	Fas/CD95	619:626	arg1	presence					607:614	the presence	603:614	the presence of Fas/CD95 in lipid rafts	603:641	Here, we provide two protocols that support the presence of Fas/CD95 in lipid rafts during apoptosis, involving lipid raft isolation and confocal microscopy techniques.
32770506	2	41	theme	signaling	444:452	arg1	molecules					454:462	downstream signaling molecules	433:462	downstream signaling molecules	433:462	Death receptors and downstream signaling molecules have been reported to be recruited into these raft domains during the triggering of apoptosis.
32770506	0	42	theme	Sucrose	24:30	arg1	Centrifugation					41:54	Sucrose Gradient Centrifugation	24:54	Sucrose Gradient Centrifugation	24:54	Lipid Raft Isolation by Sucrose Gradient Centrifugation and Visualization of Raft-Located Proteins by Fluorescence Microscopy: The Use of Combined Techniques to Assess Fas/CD95 Location in Rafts During Apoptosis Triggering.
32770506	4	43	theme	composition	957:967	arg1	analysis					933:940	analysis	933:940	analysis	933:940	A detailed protocol is provided for the isolation of lipid rafts, by taking advantage of their resistance to Triton X-100 solubilization at 4 °C, followed by subsequent sucrose gradient centrifugation and analysis of the protein composition of the different gradient fractions by Western blotting.
32770506	4	43	theme	composition	957:967	arg1	centrifugation					914:927	subsequent sucrose gradient centrifugation	886:927	subsequent sucrose gradient centrifugation	886:927	A detailed protocol is provided for the isolation of lipid rafts, by taking advantage of their resistance to Triton X-100 solubilization at 4 °C, followed by subsequent sucrose gradient centrifugation and analysis of the protein composition of the different gradient fractions by Western blotting.
32770506	5	44	theme	coclustering	1104:1115	arg1	visualization					1083:1095	the visualization	1079:1095	the visualization of the coclustering of Fas/CD95 death receptor and lipid rafts	1079:1158	In addition, we also provide a detailed protocol for the visualization of the coclustering of Fas/CD95 death receptor and lipid rafts, as assessed by using anti-Fas/CD95 antibodies and fluorescent dye-conjugated cholera toxin B subunit that binds to ganglioside GM1, a main component of lipid rafts, by immunofluorescence and confocal microscopy.
32770506	1	45	theme	Lipid	224:228	arg1	rafts					230:234	Lipid rafts	224:234	Lipid rafts	224:234	Lipid rafts are heterogeneous membrane domains enriched in cholesterol, sphingolipids, and gangliosides that serve as sorting platforms to compartmentalize and modulate signaling pathways.
32770506	1	45	theme	Lipid	224:228	arg1	domains					263:269	heterogeneous membrane domains	240:269	heterogeneous membrane domains enriched in cholesterol, sphingolipids, and gangliosides that serve as sorting platforms to compartmentalize and modulate signaling pathways	240:410	Lipid rafts are heterogeneous membrane domains enriched in cholesterol, sphingolipids, and gangliosides that serve as sorting platforms to compartmentalize and modulate signaling pathways.
32770506	4	46	theme	different	976:984	arg1	fractions					995:1003	the different gradient fractions	972:1003	the different gradient fractions	972:1003	A detailed protocol is provided for the isolation of lipid rafts, by taking advantage of their resistance to Triton X-100 solubilization at 4 °C, followed by subsequent sucrose gradient centrifugation and analysis of the protein composition of the different gradient fractions by Western blotting.
32770506	5	47	theme	receptor	1135:1142	arg1	coclustering					1104:1115	the coclustering	1100:1115	the coclustering of Fas/CD95 death receptor and lipid rafts	1100:1158	In addition, we also provide a detailed protocol for the visualization of the coclustering of Fas/CD95 death receptor and lipid rafts, as assessed by using anti-Fas/CD95 antibodies and fluorescent dye-conjugated cholera toxin B subunit that binds to ganglioside GM1, a main component of lipid rafts, by immunofluorescence and confocal microscopy.
32770506	6	48	theme	interest	1423:1430	arg1	protein					1412:1418	any protein	1408:1418	any protein of interest to be analyzed for its association to lipid rafts	1408:1480	These protocols can be extended to any protein of interest to be analyzed for its association to lipid rafts.
32770506	0	49	theme	Techniques	147:156	arg1	Use					131:133	The Use	127:133	The Use of Combined Techniques	127:156	Lipid Raft Isolation by Sucrose Gradient Centrifugation and Visualization of Raft-Located Proteins by Fluorescence Microscopy: The Use of Combined Techniques to Assess Fas/CD95 Location in Rafts During Apoptosis Triggering.
32770506	3	50	theme	confocal	696:703	arg1	microscopy					705:714	confocal microscopy	696:714	confocal microscopy	696:714	Here, we provide two protocols that support the presence of Fas/CD95 in lipid rafts during apoptosis, involving lipid raft isolation and confocal microscopy techniques.
32770506	4	51	theme	X-100	844:848	arg1	solubilization					850:863	Triton X-100 solubilization	837:863	Triton X-100 solubilization	837:863	A detailed protocol is provided for the isolation of lipid rafts, by taking advantage of their resistance to Triton X-100 solubilization at 4 °C, followed by subsequent sucrose gradient centrifugation and analysis of the protein composition of the different gradient fractions by Western blotting.
32770506	4	52	theme	gradient	905:912	arg1	centrifugation					914:927	subsequent sucrose gradient centrifugation	886:927	subsequent sucrose gradient centrifugation	886:927	A detailed protocol is provided for the isolation of lipid rafts, by taking advantage of their resistance to Triton X-100 solubilization at 4 °C, followed by subsequent sucrose gradient centrifugation and analysis of the protein composition of the different gradient fractions by Western blotting.
32770506	5	53	theme	anti-Fas/CD95	1182:1194	arg1	antibodies					1196:1205	anti-Fas/CD95 antibodies	1182:1205	anti-Fas/CD95 antibodies	1182:1205	In addition, we also provide a detailed protocol for the visualization of the coclustering of Fas/CD95 death receptor and lipid rafts, as assessed by using anti-Fas/CD95 antibodies and fluorescent dye-conjugated cholera toxin B subunit that binds to ganglioside GM1, a main component of lipid rafts, by immunofluorescence and confocal microscopy.
32770506	0	54	theme	Fas/CD95	168:175	arg1	Location					177:184	Fas/CD95 Location	168:184	Fas/CD95 Location in Rafts	168:193	Lipid Raft Isolation by Sucrose Gradient Centrifugation and Visualization of Raft-Located Proteins by Fluorescence Microscopy: The Use of Combined Techniques to Assess Fas/CD95 Location in Rafts During Apoptosis Triggering.
32770506	3	55	theme	microscopy	705:714	arg1	techniques					716:725	lipid raft isolation and confocal microscopy techniques	671:725	lipid raft isolation and confocal microscopy techniques	671:725	Here, we provide two protocols that support the presence of Fas/CD95 in lipid rafts during apoptosis, involving lipid raft isolation and confocal microscopy techniques.
32770506	3	56	theme	isolation	682:690	arg1	techniques					716:725	lipid raft isolation and confocal microscopy techniques	671:725	lipid raft isolation and confocal microscopy techniques	671:725	Here, we provide two protocols that support the presence of Fas/CD95 in lipid rafts during apoptosis, involving lipid raft isolation and confocal microscopy techniques.
32770506	5	57	theme	lipid	1313:1317	arg1	rafts					1319:1323	lipid rafts	1313:1323	lipid rafts	1313:1323	In addition, we also provide a detailed protocol for the visualization of the coclustering of Fas/CD95 death receptor and lipid rafts, as assessed by using anti-Fas/CD95 antibodies and fluorescent dye-conjugated cholera toxin B subunit that binds to ganglioside GM1, a main component of lipid rafts, by immunofluorescence and confocal microscopy.
32770506	4	58	theme	Western	1008:1014	arg1	blotting					1016:1023	Western blotting	1008:1023	Western blotting	1008:1023	A detailed protocol is provided for the isolation of lipid rafts, by taking advantage of their resistance to Triton X-100 solubilization at 4 °C, followed by subsequent sucrose gradient centrifugation and analysis of the protein composition of the different gradient fractions by Western blotting.
32770506	2	59	theme	downstream	433:442	arg1	molecules					454:462	downstream signaling molecules	433:462	downstream signaling molecules	433:462	Death receptors and downstream signaling molecules have been reported to be recruited into these raft domains during the triggering of apoptosis.
32770506	3	60	theme	lipid	631:635	arg1	rafts					637:641	lipid rafts	631:641	lipid rafts	631:641	Here, we provide two protocols that support the presence of Fas/CD95 in lipid rafts during apoptosis, involving lipid raft isolation and confocal microscopy techniques.
32770506	5	61	theme	rafts	1319:1323	arg1	GM1					1288:1290	ganglioside GM1	1276:1290	ganglioside GM1	1276:1290	In addition, we also provide a detailed protocol for the visualization of the coclustering of Fas/CD95 death receptor and lipid rafts, as assessed by using anti-Fas/CD95 antibodies and fluorescent dye-conjugated cholera toxin B subunit that binds to ganglioside GM1, a main component of lipid rafts, by immunofluorescence and confocal microscopy.
32770506	5	61	theme	rafts	1319:1323	arg1	component					1300:1308	a main component	1293:1308	a main component of lipid rafts	1293:1323	In addition, we also provide a detailed protocol for the visualization of the coclustering of Fas/CD95 death receptor and lipid rafts, as assessed by using anti-Fas/CD95 antibodies and fluorescent dye-conjugated cholera toxin B subunit that binds to ganglioside GM1, a main component of lipid rafts, by immunofluorescence and confocal microscopy.
32770506	1	62	theme	heterogeneous	240:252	arg1	rafts					230:234	Lipid rafts	224:234	Lipid rafts	224:234	Lipid rafts are heterogeneous membrane domains enriched in cholesterol, sphingolipids, and gangliosides that serve as sorting platforms to compartmentalize and modulate signaling pathways.
32770506	1	62	theme	heterogeneous	240:252	arg1	domains					263:269	heterogeneous membrane domains	240:269	heterogeneous membrane domains enriched in cholesterol, sphingolipids, and gangliosides that serve as sorting platforms to compartmentalize and modulate signaling pathways	240:410	Lipid rafts are heterogeneous membrane domains enriched in cholesterol, sphingolipids, and gangliosides that serve as sorting platforms to compartmentalize and modulate signaling pathways.
32770506	1	63	theme	sorting	342:348	arg1	sphingolipids					296:308	sphingolipids	296:308	sphingolipids	296:308	Lipid rafts are heterogeneous membrane domains enriched in cholesterol, sphingolipids, and gangliosides that serve as sorting platforms to compartmentalize and modulate signaling pathways.
32770506	1	63	theme	sorting	342:348	arg1	platforms					350:358	sorting platforms	342:358	sorting platforms	342:358	Lipid rafts are heterogeneous membrane domains enriched in cholesterol, sphingolipids, and gangliosides that serve as sorting platforms to compartmentalize and modulate signaling pathways.
32770506	1	63	theme	sorting	342:348	arg1	gangliosides					315:326	gangliosides	315:326	gangliosides	315:326	Lipid rafts are heterogeneous membrane domains enriched in cholesterol, sphingolipids, and gangliosides that serve as sorting platforms to compartmentalize and modulate signaling pathways.
32770506	1	63	theme	sorting	342:348	arg1	cholesterol					283:293	cholesterol	283:293	cholesterol	283:293	Lipid rafts are heterogeneous membrane domains enriched in cholesterol, sphingolipids, and gangliosides that serve as sorting platforms to compartmentalize and modulate signaling pathways.
32770506	5	64	theme	ganglioside	1276:1286	arg1	GM1					1288:1290	ganglioside GM1	1276:1290	ganglioside GM1	1276:1290	In addition, we also provide a detailed protocol for the visualization of the coclustering of Fas/CD95 death receptor and lipid rafts, as assessed by using anti-Fas/CD95 antibodies and fluorescent dye-conjugated cholera toxin B subunit that binds to ganglioside GM1, a main component of lipid rafts, by immunofluorescence and confocal microscopy.
32770506	5	64	theme	ganglioside	1276:1286	arg1	component					1300:1308	a main component	1293:1308	a main component of lipid rafts	1293:1323	In addition, we also provide a detailed protocol for the visualization of the coclustering of Fas/CD95 death receptor and lipid rafts, as assessed by using anti-Fas/CD95 antibodies and fluorescent dye-conjugated cholera toxin B subunit that binds to ganglioside GM1, a main component of lipid rafts, by immunofluorescence and confocal microscopy.
32770506	1	65	theme	membrane	254:261	arg1	rafts					230:234	Lipid rafts	224:234	Lipid rafts	224:234	Lipid rafts are heterogeneous membrane domains enriched in cholesterol, sphingolipids, and gangliosides that serve as sorting platforms to compartmentalize and modulate signaling pathways.
32770506	1	65	theme	membrane	254:261	arg1	domains					263:269	heterogeneous membrane domains	240:269	heterogeneous membrane domains enriched in cholesterol, sphingolipids, and gangliosides that serve as sorting platforms to compartmentalize and modulate signaling pathways	240:410	Lipid rafts are heterogeneous membrane domains enriched in cholesterol, sphingolipids, and gangliosides that serve as sorting platforms to compartmentalize and modulate signaling pathways.
32228773	0	0	theme	Jiangella	83:91	arg1	sp					102:103	Jiangella asiatica sp	83:103	Genome-based classification of three novel actinobacteria from the Karakum Desert: Jiangella asiatica sp.	0:104	Genome-based classification of three novel actinobacteria from the Karakum Desert: Jiangella asiatica sp.
32228773	13	1	theme	33520T=CGMCC	1771:1782	arg1	KC603T					1758:1763	Jiangella ureilytica KC603T	1737:1763	Jiangella ureilytica KC603T (=JCM 33520T=CGMCC 4.7618T)	1737:1791	The type strains of the proposed novel species are as follows: Jiangella asiatica 5K138T (=JCM 33518T=CGMCC 4.7672T), Jiangella aurantiaca 8K307T (=JCM 33519T=CGMCC 4.7621T) and Jiangella ureilytica KC603T (=JCM 33520T=CGMCC 4.7618T).
32228773	13	1	theme	33520T=CGMCC	1771:1782	arg1	4.7618T					1784:1790	=JCM 33520T=CGMCC 4.7618T	1766:1790	=JCM 33520T=CGMCC 4.7618T	1766:1790	The type strains of the proposed novel species are as follows: Jiangella asiatica 5K138T (=JCM 33518T=CGMCC 4.7672T), Jiangella aurantiaca 8K307T (=JCM 33519T=CGMCC 4.7621T) and Jiangella ureilytica KC603T (=JCM 33520T=CGMCC 4.7618T).
32228773	9	2	theme	novel	1376:1380	arg1	species					1382:1388	a novel species	1374:1388	a novel species	1374:1388	Therefore, strains 5K138T, 8K307T and KC603T should each be classified as representing a novel species within the genus Jiangella, for which the names Jiangella asiatica sp.
32228773	13	3	theme	=JCM	1649:1652	arg1	4.7672T					1667:1673	=JCM 33518T=CGMCC 4.7672T	1649:1673	=JCM 33518T=CGMCC 4.7672T	1649:1673	The type strains of the proposed novel species are as follows: Jiangella asiatica 5K138T (=JCM 33518T=CGMCC 4.7672T), Jiangella aurantiaca 8K307T (=JCM 33519T=CGMCC 4.7621T) and Jiangella ureilytica KC603T (=JCM 33520T=CGMCC 4.7618T).
32228773	13	3	theme	=JCM	1649:1652	arg1	follows					1613:1619	follows	1613:1619	follows	1613:1619	The type strains of the proposed novel species are as follows: Jiangella asiatica 5K138T (=JCM 33518T=CGMCC 4.7672T), Jiangella aurantiaca 8K307T (=JCM 33519T=CGMCC 4.7621T) and Jiangella ureilytica KC603T (=JCM 33520T=CGMCC 4.7618T).
32228773	2	4	dep	sp	167:168	arg1	nov.					137:140	nov.	137:140	nov.	137:140	nov. and Jiangella ureilytica sp.
32228773	2	4	dep	sp	167:168	arg1	Jiangella					146:154	Jiangella	146:154	Jiangella	146:154	nov. and Jiangella ureilytica sp.
32228773	8	5	theme	taxonomic	1189:1197	arg1	positions					1199:1207	the taxonomic positions	1185:1207	the taxonomic positions of these strains	1185:1224	However, a combination of phenotypic and phylogenetic approaches as well as genome-based comparative analyses confirmed the taxonomic positions of these strains as representing distinct species within the genus Jiangella.
32228773	0	6	from	classification	13:26	arg1	Desert					75:80	the Karakum Desert	63:80	the Karakum Desert	63:80	Genome-based classification of three novel actinobacteria from the Karakum Desert: Jiangella asiatica sp.
32228773	11	7	dep	sp	1522:1523	arg1	Jiangella					1501:1509	Jiangella	1501:1509	Jiangella	1501:1509	nov. and Jiangella ureilytica sp.
32228773	11	7	dep	sp	1522:1523	arg1	nov.					1492:1495	nov.	1492:1495	nov.	1492:1495	nov. and Jiangella ureilytica sp.
32228773	13	8	theme	ureilytica	1747:1756	arg1	KC603T					1758:1763	Jiangella ureilytica KC603T	1737:1763	Jiangella ureilytica KC603T (=JCM 33520T=CGMCC 4.7618T)	1737:1791	The type strains of the proposed novel species are as follows: Jiangella asiatica 5K138T (=JCM 33518T=CGMCC 4.7672T), Jiangella aurantiaca 8K307T (=JCM 33519T=CGMCC 4.7621T) and Jiangella ureilytica KC603T (=JCM 33520T=CGMCC 4.7618T).
32228773	13	8	theme	ureilytica	1747:1756	arg1	4.7618T					1784:1790	=JCM 33520T=CGMCC 4.7618T	1766:1790	=JCM 33520T=CGMCC 4.7618T	1766:1790	The type strains of the proposed novel species are as follows: Jiangella asiatica 5K138T (=JCM 33518T=CGMCC 4.7672T), Jiangella aurantiaca 8K307T (=JCM 33519T=CGMCC 4.7621T) and Jiangella ureilytica KC603T (=JCM 33520T=CGMCC 4.7618T).
32228773	5	9	theme	polyphasic	415:424	arg1	approach					426:433	A polyphasic approach	413:433	A polyphasic approach	413:433	A polyphasic approach was adopted to determine taxonomic affiliations of the strains.
32228773	6	10	theme	consistent	544:553	arg1	properties					533:542	chemotaxonomic properties	518:542	chemotaxonomic properties consistent with their classification as representing members of the genus Jiangella such as ll-diaminopimelic acid in the cell wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as major polar lipids as well as MK-9(H4) as a major menaquinone	518:824	The strains showed chemotaxonomic properties consistent with their classification as representing members of the genus Jiangella such as ll-diaminopimelic acid in the cell wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as major polar lipids as well as MK-9(H4) as a major menaquinone.
32228773	10	11	theme	aurantiaca	1477:1486	arg1	sp					1488:1489	Jiangella aurantiaca sp	1467:1489	Jiangella aurantiaca sp	1467:1489	nov., Jiangella aurantiaca sp.
32228773	10	11	theme	aurantiaca	1477:1486	arg1	nov.					1461:1464	nov.	1461:1464	nov.	1461:1464	nov., Jiangella aurantiaca sp.
32228773	4	12	with	isolates	182:189	arg1	characteristics					246:260	typical morphological characteristics	224:260	typical morphological characteristics of members of the genus Jiangella	224:294	Three isolates, 5K138T, 8K307T and KC603T, with typical morphological characteristics of members of the genus Jiangella were obtained during a study searching for novel actinobacteria with biosynthetic potential from the Karakum Desert.
32228773	6	13	theme	ll-diaminopimelic	636:652	arg1	acid					654:657	ll-diaminopimelic acid	636:657	ll-diaminopimelic acid in the cell wall peptidoglycan	636:688	The strains showed chemotaxonomic properties consistent with their classification as representing members of the genus Jiangella such as ll-diaminopimelic acid in the cell wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as major polar lipids as well as MK-9(H4) as a major menaquinone.
32228773	0	14	theme	asiatica	93:100	arg1	sp					102:103	Jiangella asiatica sp	83:103	Genome-based classification of three novel actinobacteria from the Karakum Desert: Jiangella asiatica sp.	0:104	Genome-based classification of three novel actinobacteria from the Karakum Desert: Jiangella asiatica sp.
32228773	10	15	theme	Jiangella	1467:1475	arg1	sp					1488:1489	Jiangella aurantiaca sp	1467:1489	Jiangella aurantiaca sp	1467:1489	nov., Jiangella aurantiaca sp.
32228773	10	15	theme	Jiangella	1467:1475	arg1	nov.					1461:1464	nov.	1461:1464	nov.	1461:1464	nov., Jiangella aurantiaca sp.
32228773	13	16	dep	follows	1613:1619	arg1	5K138T					1641:1646	5K138T	1641:1646	5K138T	1641:1646	The type strains of the proposed novel species are as follows: Jiangella asiatica 5K138T (=JCM 33518T=CGMCC 4.7672T), Jiangella aurantiaca 8K307T (=JCM 33519T=CGMCC 4.7621T) and Jiangella ureilytica KC603T (=JCM 33520T=CGMCC 4.7618T).
32228773	5	17	theme	strains	490:496	arg1	affiliations					470:481	taxonomic affiliations	460:481	taxonomic affiliations of the strains	460:496	A polyphasic approach was adopted to determine taxonomic affiliations of the strains.
32228773	7	18	dep	identities	1053:1062	arg1	%					1042:1042	%	1042:1042	%	1042:1042	Pairwise sequence comparisons of the 16S rRNA genes showed that the strains were closely related to Jiangella alba DSM 45237T, Jiangella rhizosphaerae NEAU-YY265T and Jiangella mangrovi 3SM4-07T with higher than 99 % sequence identities.
32228773	6	19	theme	chemotaxonomic	518:531	arg1	properties					533:542	chemotaxonomic properties	518:542	chemotaxonomic properties consistent with their classification as representing members of the genus Jiangella such as ll-diaminopimelic acid in the cell wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as major polar lipids as well as MK-9(H4) as a major menaquinone	518:824	The strains showed chemotaxonomic properties consistent with their classification as representing members of the genus Jiangella such as ll-diaminopimelic acid in the cell wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as major polar lipids as well as MK-9(H4) as a major menaquinone.
32228773	6	20	theme	polar	770:774	arg1	lipids					776:781	major polar lipids	764:781	major polar lipids as well as MK-9(H4)	764:801	The strains showed chemotaxonomic properties consistent with their classification as representing members of the genus Jiangella such as ll-diaminopimelic acid in the cell wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as major polar lipids as well as MK-9(H4) as a major menaquinone.
32228773	6	20	theme	polar	770:774	arg1	members					597:603	members	597:603	members of the genus Jiangella such as ll-diaminopimelic acid in the cell wall peptidoglycan	597:688	The strains showed chemotaxonomic properties consistent with their classification as representing members of the genus Jiangella such as ll-diaminopimelic acid in the cell wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as major polar lipids as well as MK-9(H4) as a major menaquinone.
32228773	6	20	theme	polar	770:774	arg1	acid					654:657	ll-diaminopimelic acid	636:657	ll-diaminopimelic acid in the cell wall peptidoglycan	636:688	The strains showed chemotaxonomic properties consistent with their classification as representing members of the genus Jiangella such as ll-diaminopimelic acid in the cell wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as major polar lipids as well as MK-9(H4) as a major menaquinone.
32228773	4	21	theme	Karakum	397:403	arg1	Desert					405:410	the Karakum Desert	393:410	the Karakum Desert	393:410	Three isolates, 5K138T, 8K307T and KC603T, with typical morphological characteristics of members of the genus Jiangella were obtained during a study searching for novel actinobacteria with biosynthetic potential from the Karakum Desert.
32228773	6	22	theme	wall	671:674	arg1	peptidoglycan					676:688	the cell wall peptidoglycan	662:688	the cell wall peptidoglycan	662:688	The strains showed chemotaxonomic properties consistent with their classification as representing members of the genus Jiangella such as ll-diaminopimelic acid in the cell wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as major polar lipids as well as MK-9(H4) as a major menaquinone.
32228773	8	23	theme	strains	1218:1224	arg1	positions					1199:1207	the taxonomic positions	1185:1207	the taxonomic positions of these strains	1185:1224	However, a combination of phenotypic and phylogenetic approaches as well as genome-based comparative analyses confirmed the taxonomic positions of these strains as representing distinct species within the genus Jiangella.
32228773	8	24	theme	genus	1270:1274	arg1	Jiangella					1276:1284	the genus Jiangella	1266:1284	the genus Jiangella	1266:1284	However, a combination of phenotypic and phylogenetic approaches as well as genome-based comparative analyses confirmed the taxonomic positions of these strains as representing distinct species within the genus Jiangella.
32228773	4	25	dep	typical	224:230	arg1	morphological					232:244	morphological	232:244	morphological	232:244	Three isolates, 5K138T, 8K307T and KC603T, with typical morphological characteristics of members of the genus Jiangella were obtained during a study searching for novel actinobacteria with biosynthetic potential from the Karakum Desert.
32228773	4	26	theme	Jiangella	286:294	arg1	members					265:271	members	265:271	members of the genus Jiangella	265:294	Three isolates, 5K138T, 8K307T and KC603T, with typical morphological characteristics of members of the genus Jiangella were obtained during a study searching for novel actinobacteria with biosynthetic potential from the Karakum Desert.
32228773	8	27	theme	distinct	1242:1249	arg1	species					1251:1257	distinct species	1242:1257	distinct species	1242:1257	However, a combination of phenotypic and phylogenetic approaches as well as genome-based comparative analyses confirmed the taxonomic positions of these strains as representing distinct species within the genus Jiangella.
32228773	7	28	theme	Pairwise	827:834	arg1	comparisons					845:855	Pairwise sequence comparisons	827:855	Pairwise sequence comparisons of the 16S rRNA genes	827:877	Pairwise sequence comparisons of the 16S rRNA genes showed that the strains were closely related to Jiangella alba DSM 45237T, Jiangella rhizosphaerae NEAU-YY265T and Jiangella mangrovi 3SM4-07T with higher than 99 % sequence identities.
32228773	13	29	theme	33519T=CGMCC	1711:1722	arg1	4.7621T					1724:1730	=JCM 33519T=CGMCC 4.7621T	1706:1730	=JCM 33519T=CGMCC 4.7621T	1706:1730	The type strains of the proposed novel species are as follows: Jiangella asiatica 5K138T (=JCM 33518T=CGMCC 4.7672T), Jiangella aurantiaca 8K307T (=JCM 33519T=CGMCC 4.7621T) and Jiangella ureilytica KC603T (=JCM 33520T=CGMCC 4.7618T).
32228773	13	29	theme	33519T=CGMCC	1711:1722	arg1	8K307T					1698:1703	Jiangella aurantiaca 8K307T	1677:1703	Jiangella aurantiaca 8K307T (=JCM 33519T=CGMCC 4.7621T)	1677:1731	The type strains of the proposed novel species are as follows: Jiangella asiatica 5K138T (=JCM 33518T=CGMCC 4.7672T), Jiangella aurantiaca 8K307T (=JCM 33519T=CGMCC 4.7621T) and Jiangella ureilytica KC603T (=JCM 33520T=CGMCC 4.7618T).
32228773	6	30	theme	major	808:812	arg1	menaquinone					814:824	a major menaquinone	806:824	a major menaquinone	806:824	The strains showed chemotaxonomic properties consistent with their classification as representing members of the genus Jiangella such as ll-diaminopimelic acid in the cell wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as major polar lipids as well as MK-9(H4) as a major menaquinone.
32228773	4	31	theme	genus	280:284	arg1	Jiangella					286:294	the genus Jiangella	276:294	the genus Jiangella	276:294	Three isolates, 5K138T, 8K307T and KC603T, with typical morphological characteristics of members of the genus Jiangella were obtained during a study searching for novel actinobacteria with biosynthetic potential from the Karakum Desert.
32228773	0	32	theme	Genome-based	0:11	arg1	classification					13:26	Genome-based classification	0:26	Genome-based classification of three novel actinobacteria from the Karakum Desert: Jiangella asiatica sp.	0:104	Genome-based classification of three novel actinobacteria from the Karakum Desert: Jiangella asiatica sp.
32228773	7	33	theme	sequence	836:843	arg1	comparisons					845:855	Pairwise sequence comparisons	827:855	Pairwise sequence comparisons of the 16S rRNA genes	827:877	Pairwise sequence comparisons of the 16S rRNA genes showed that the strains were closely related to Jiangella alba DSM 45237T, Jiangella rhizosphaerae NEAU-YY265T and Jiangella mangrovi 3SM4-07T with higher than 99 % sequence identities.
32228773	8	34	theme	phenotypic	1091:1100	arg1	approaches					1119:1128	phenotypic and phylogenetic approaches	1091:1128	phenotypic and phylogenetic approaches	1091:1128	However, a combination of phenotypic and phylogenetic approaches as well as genome-based comparative analyses confirmed the taxonomic positions of these strains as representing distinct species within the genus Jiangella.
32228773	5	35	theme	taxonomic	460:468	arg1	affiliations					470:481	taxonomic affiliations	460:481	taxonomic affiliations of the strains	460:496	A polyphasic approach was adopted to determine taxonomic affiliations of the strains.
32228773	6	36	theme	Jiangella	618:626	arg1	acid					654:657	ll-diaminopimelic acid	636:657	ll-diaminopimelic acid in the cell wall peptidoglycan	636:688	The strains showed chemotaxonomic properties consistent with their classification as representing members of the genus Jiangella such as ll-diaminopimelic acid in the cell wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as major polar lipids as well as MK-9(H4) as a major menaquinone.
32228773	6	36	theme	Jiangella	618:626	arg1	diphosphatidylglycerol					691:712	diphosphatidylglycerol	691:712	diphosphatidylglycerol	691:712	The strains showed chemotaxonomic properties consistent with their classification as representing members of the genus Jiangella such as ll-diaminopimelic acid in the cell wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as major polar lipids as well as MK-9(H4) as a major menaquinone.
32228773	6	36	theme	Jiangella	618:626	arg1	phosphatidylglycerol					715:734	phosphatidylglycerol	715:734	phosphatidylglycerol	715:734	The strains showed chemotaxonomic properties consistent with their classification as representing members of the genus Jiangella such as ll-diaminopimelic acid in the cell wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as major polar lipids as well as MK-9(H4) as a major menaquinone.
32228773	6	36	theme	Jiangella	618:626	arg1	lipids					776:781	major polar lipids	764:781	major polar lipids as well as MK-9(H4)	764:801	The strains showed chemotaxonomic properties consistent with their classification as representing members of the genus Jiangella such as ll-diaminopimelic acid in the cell wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as major polar lipids as well as MK-9(H4) as a major menaquinone.
32228773	6	36	theme	Jiangella	618:626	arg1	members					597:603	members	597:603	members of the genus Jiangella such as ll-diaminopimelic acid in the cell wall peptidoglycan	597:688	The strains showed chemotaxonomic properties consistent with their classification as representing members of the genus Jiangella such as ll-diaminopimelic acid in the cell wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as major polar lipids as well as MK-9(H4) as a major menaquinone.
32228773	6	36	theme	Jiangella	618:626	arg1	phosphatidylinositol					740:759	phosphatidylinositol	740:759	phosphatidylinositol	740:759	The strains showed chemotaxonomic properties consistent with their classification as representing members of the genus Jiangella such as ll-diaminopimelic acid in the cell wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as major polar lipids as well as MK-9(H4) as a major menaquinone.
32228773	9	37	dep	classified	1347:1356	arg1	each					1339:1342	each	1339:1342	each	1339:1342	Therefore, strains 5K138T, 8K307T and KC603T should each be classified as representing a novel species within the genus Jiangella, for which the names Jiangella asiatica sp.
32228773	8	38	theme	approaches	1119:1128	arg1	combination					1076:1086	a combination	1074:1086	a combination of phenotypic and phylogenetic approaches as well as genome-based comparative analyses	1074:1173	However, a combination of phenotypic and phylogenetic approaches as well as genome-based comparative analyses confirmed the taxonomic positions of these strains as representing distinct species within the genus Jiangella.
32228773	8	38	theme	approaches	1119:1128	arg1	analyses					1166:1173	genome-based comparative analyses	1141:1173	a combination of phenotypic and phylogenetic approaches as well as genome-based comparative analyses	1074:1173	However, a combination of phenotypic and phylogenetic approaches as well as genome-based comparative analyses confirmed the taxonomic positions of these strains as representing distinct species within the genus Jiangella.
32228773	4	39	theme	biosynthetic	365:376	arg1	potential					378:386	biosynthetic potential	365:386	biosynthetic potential	365:386	Three isolates, 5K138T, 8K307T and KC603T, with typical morphological characteristics of members of the genus Jiangella were obtained during a study searching for novel actinobacteria with biosynthetic potential from the Karakum Desert.
32228773	7	40	theme	rRNA	868:871	arg1	genes					873:877	the 16S rRNA genes	860:877	the 16S rRNA genes	860:877	Pairwise sequence comparisons of the 16S rRNA genes showed that the strains were closely related to Jiangella alba DSM 45237T, Jiangella rhizosphaerae NEAU-YY265T and Jiangella mangrovi 3SM4-07T with higher than 99 % sequence identities.
32228773	6	41	theme	genus	612:616	arg1	Jiangella					618:626	the genus Jiangella	608:626	the genus Jiangella	608:626	The strains showed chemotaxonomic properties consistent with their classification as representing members of the genus Jiangella such as ll-diaminopimelic acid in the cell wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as major polar lipids as well as MK-9(H4) as a major menaquinone.
32228773	13	42	theme	Jiangella	1737:1745	arg1	KC603T					1758:1763	Jiangella ureilytica KC603T	1737:1763	Jiangella ureilytica KC603T (=JCM 33520T=CGMCC 4.7618T)	1737:1791	The type strains of the proposed novel species are as follows: Jiangella asiatica 5K138T (=JCM 33518T=CGMCC 4.7672T), Jiangella aurantiaca 8K307T (=JCM 33519T=CGMCC 4.7621T) and Jiangella ureilytica KC603T (=JCM 33520T=CGMCC 4.7618T).
32228773	13	42	theme	Jiangella	1737:1745	arg1	4.7618T					1784:1790	=JCM 33520T=CGMCC 4.7618T	1766:1790	=JCM 33520T=CGMCC 4.7618T	1766:1790	The type strains of the proposed novel species are as follows: Jiangella asiatica 5K138T (=JCM 33518T=CGMCC 4.7672T), Jiangella aurantiaca 8K307T (=JCM 33519T=CGMCC 4.7621T) and Jiangella ureilytica KC603T (=JCM 33520T=CGMCC 4.7618T).
32228773	8	43	theme	phylogenetic	1106:1117	arg1	approaches					1119:1128	phenotypic and phylogenetic approaches	1091:1128	phenotypic and phylogenetic approaches	1091:1128	However, a combination of phenotypic and phylogenetic approaches as well as genome-based comparative analyses confirmed the taxonomic positions of these strains as representing distinct species within the genus Jiangella.
32228773	13	44	theme	Jiangella	1677:1685	arg1	4.7621T					1724:1730	=JCM 33519T=CGMCC 4.7621T	1706:1730	=JCM 33519T=CGMCC 4.7621T	1706:1730	The type strains of the proposed novel species are as follows: Jiangella asiatica 5K138T (=JCM 33518T=CGMCC 4.7672T), Jiangella aurantiaca 8K307T (=JCM 33519T=CGMCC 4.7621T) and Jiangella ureilytica KC603T (=JCM 33520T=CGMCC 4.7618T).
32228773	13	44	theme	Jiangella	1677:1685	arg1	8K307T					1698:1703	Jiangella aurantiaca 8K307T	1677:1703	Jiangella aurantiaca 8K307T (=JCM 33519T=CGMCC 4.7621T)	1677:1731	The type strains of the proposed novel species are as follows: Jiangella asiatica 5K138T (=JCM 33518T=CGMCC 4.7672T), Jiangella aurantiaca 8K307T (=JCM 33519T=CGMCC 4.7621T) and Jiangella ureilytica KC603T (=JCM 33520T=CGMCC 4.7618T).
32228773	9	45	theme	genus	1401:1405	arg1	Jiangella					1407:1415	the genus Jiangella	1397:1415	the genus Jiangella	1397:1415	Therefore, strains 5K138T, 8K307T and KC603T should each be classified as representing a novel species within the genus Jiangella, for which the names Jiangella asiatica sp.
32228773	4	46	theme	members	265:271	arg1	characteristics					246:260	typical morphological characteristics	224:260	typical morphological characteristics of members of the genus Jiangella	224:294	Three isolates, 5K138T, 8K307T and KC603T, with typical morphological characteristics of members of the genus Jiangella were obtained during a study searching for novel actinobacteria with biosynthetic potential from the Karakum Desert.
32228773	7	47	theme	16S	864:866	arg1	genes					873:877	the 16S rRNA genes	860:877	the 16S rRNA genes	860:877	Pairwise sequence comparisons of the 16S rRNA genes showed that the strains were closely related to Jiangella alba DSM 45237T, Jiangella rhizosphaerae NEAU-YY265T and Jiangella mangrovi 3SM4-07T with higher than 99 % sequence identities.
32228773	0	48	theme	actinobacteria	43:56	arg1	classification					13:26	Genome-based classification	0:26	Genome-based classification of three novel actinobacteria from the Karakum Desert: Jiangella asiatica sp.	0:104	Genome-based classification of three novel actinobacteria from the Karakum Desert: Jiangella asiatica sp.
32228773	6	49	theme	major	764:768	arg1	lipids					776:781	major polar lipids	764:781	major polar lipids as well as MK-9(H4)	764:801	The strains showed chemotaxonomic properties consistent with their classification as representing members of the genus Jiangella such as ll-diaminopimelic acid in the cell wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as major polar lipids as well as MK-9(H4) as a major menaquinone.
32228773	6	49	theme	major	764:768	arg1	members					597:603	members	597:603	members of the genus Jiangella such as ll-diaminopimelic acid in the cell wall peptidoglycan	597:688	The strains showed chemotaxonomic properties consistent with their classification as representing members of the genus Jiangella such as ll-diaminopimelic acid in the cell wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as major polar lipids as well as MK-9(H4) as a major menaquinone.
32228773	6	49	theme	major	764:768	arg1	acid					654:657	ll-diaminopimelic acid	636:657	ll-diaminopimelic acid in the cell wall peptidoglycan	636:688	The strains showed chemotaxonomic properties consistent with their classification as representing members of the genus Jiangella such as ll-diaminopimelic acid in the cell wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as major polar lipids as well as MK-9(H4) as a major menaquinone.
32228773	13	50	theme	aurantiaca	1687:1696	arg1	4.7621T					1724:1730	=JCM 33519T=CGMCC 4.7621T	1706:1730	=JCM 33519T=CGMCC 4.7621T	1706:1730	The type strains of the proposed novel species are as follows: Jiangella asiatica 5K138T (=JCM 33518T=CGMCC 4.7672T), Jiangella aurantiaca 8K307T (=JCM 33519T=CGMCC 4.7621T) and Jiangella ureilytica KC603T (=JCM 33520T=CGMCC 4.7618T).
32228773	13	50	theme	aurantiaca	1687:1696	arg1	8K307T					1698:1703	Jiangella aurantiaca 8K307T	1677:1703	Jiangella aurantiaca 8K307T (=JCM 33519T=CGMCC 4.7621T)	1677:1731	The type strains of the proposed novel species are as follows: Jiangella asiatica 5K138T (=JCM 33518T=CGMCC 4.7672T), Jiangella aurantiaca 8K307T (=JCM 33519T=CGMCC 4.7621T) and Jiangella ureilytica KC603T (=JCM 33520T=CGMCC 4.7618T).
32228773	7	51	dep	%	1042:1042	arg1	99 					1039:1041	99 	1039:1041	99 	1039:1041	Pairwise sequence comparisons of the 16S rRNA genes showed that the strains were closely related to Jiangella alba DSM 45237T, Jiangella rhizosphaerae NEAU-YY265T and Jiangella mangrovi 3SM4-07T with higher than 99 % sequence identities.
32228773	0	52	theme	novel	37:41	arg1	actinobacteria					43:56	three novel actinobacteria	31:56	three novel actinobacteria from the Karakum Desert	31:80	Genome-based classification of three novel actinobacteria from the Karakum Desert: Jiangella asiatica sp.
32228773	0	53	from	Desert	75:80	arg1	actinobacteria					43:56	three novel actinobacteria	31:56	three novel actinobacteria from the Karakum Desert	31:80	Genome-based classification of three novel actinobacteria from the Karakum Desert: Jiangella asiatica sp.
32228773	0	53	from	Desert	75:80	arg1	classification					13:26	Genome-based classification	0:26	Genome-based classification of three novel actinobacteria from the Karakum Desert: Jiangella asiatica sp.	0:104	Genome-based classification of three novel actinobacteria from the Karakum Desert: Jiangella asiatica sp.
32228773	13	54	theme	proposed	1583:1590	arg1	species					1598:1604	the proposed novel species	1579:1604	the proposed novel species	1579:1604	The type strains of the proposed novel species are as follows: Jiangella asiatica 5K138T (=JCM 33518T=CGMCC 4.7672T), Jiangella aurantiaca 8K307T (=JCM 33519T=CGMCC 4.7621T) and Jiangella ureilytica KC603T (=JCM 33520T=CGMCC 4.7618T).
32228773	7	55	theme	sequence	1044:1051	arg1	identities					1053:1062	higher than 99 % sequence identities	1027:1062	higher than 99 % sequence identities	1027:1062	Pairwise sequence comparisons of the 16S rRNA genes showed that the strains were closely related to Jiangella alba DSM 45237T, Jiangella rhizosphaerae NEAU-YY265T and Jiangella mangrovi 3SM4-07T with higher than 99 % sequence identities.
32228773	13	56	theme	=JCM	1766:1769	arg1	KC603T					1758:1763	Jiangella ureilytica KC603T	1737:1763	Jiangella ureilytica KC603T (=JCM 33520T=CGMCC 4.7618T)	1737:1791	The type strains of the proposed novel species are as follows: Jiangella asiatica 5K138T (=JCM 33518T=CGMCC 4.7672T), Jiangella aurantiaca 8K307T (=JCM 33519T=CGMCC 4.7621T) and Jiangella ureilytica KC603T (=JCM 33520T=CGMCC 4.7618T).
32228773	13	56	theme	=JCM	1766:1769	arg1	4.7618T					1784:1790	=JCM 33520T=CGMCC 4.7618T	1766:1790	=JCM 33520T=CGMCC 4.7618T	1766:1790	The type strains of the proposed novel species are as follows: Jiangella asiatica 5K138T (=JCM 33518T=CGMCC 4.7672T), Jiangella aurantiaca 8K307T (=JCM 33519T=CGMCC 4.7621T) and Jiangella ureilytica KC603T (=JCM 33520T=CGMCC 4.7618T).
32228773	8	57	theme	genome-based	1141:1152	arg1	analyses					1166:1173	genome-based comparative analyses	1141:1173	a combination of phenotypic and phylogenetic approaches as well as genome-based comparative analyses	1074:1173	However, a combination of phenotypic and phylogenetic approaches as well as genome-based comparative analyses confirmed the taxonomic positions of these strains as representing distinct species within the genus Jiangella.
32228773	2	58	theme	ureilytica	156:165	arg1	sp					167:168	ureilytica sp	156:168	ureilytica sp	156:168	nov. and Jiangella ureilytica sp.
32228773	13	59	theme	novel	1592:1596	arg1	species					1598:1604	the proposed novel species	1579:1604	the proposed novel species	1579:1604	The type strains of the proposed novel species are as follows: Jiangella asiatica 5K138T (=JCM 33518T=CGMCC 4.7672T), Jiangella aurantiaca 8K307T (=JCM 33519T=CGMCC 4.7621T) and Jiangella ureilytica KC603T (=JCM 33520T=CGMCC 4.7618T).
32228773	4	60	with	actinobacteria	345:358	arg1	potential					378:386	biosynthetic potential	365:386	biosynthetic potential	365:386	Three isolates, 5K138T, 8K307T and KC603T, with typical morphological characteristics of members of the genus Jiangella were obtained during a study searching for novel actinobacteria with biosynthetic potential from the Karakum Desert.
32228773	13	61	theme	33518T=CGMCC	1654:1665	arg1	4.7672T					1667:1673	=JCM 33518T=CGMCC 4.7672T	1649:1673	=JCM 33518T=CGMCC 4.7672T	1649:1673	The type strains of the proposed novel species are as follows: Jiangella asiatica 5K138T (=JCM 33518T=CGMCC 4.7672T), Jiangella aurantiaca 8K307T (=JCM 33519T=CGMCC 4.7621T) and Jiangella ureilytica KC603T (=JCM 33520T=CGMCC 4.7618T).
32228773	13	61	theme	33518T=CGMCC	1654:1665	arg1	follows					1613:1619	follows	1613:1619	follows	1613:1619	The type strains of the proposed novel species are as follows: Jiangella asiatica 5K138T (=JCM 33518T=CGMCC 4.7672T), Jiangella aurantiaca 8K307T (=JCM 33519T=CGMCC 4.7621T) and Jiangella ureilytica KC603T (=JCM 33520T=CGMCC 4.7618T).
32228773	13	62	theme	=JCM	1706:1709	arg1	4.7621T					1724:1730	=JCM 33519T=CGMCC 4.7621T	1706:1730	=JCM 33519T=CGMCC 4.7621T	1706:1730	The type strains of the proposed novel species are as follows: Jiangella asiatica 5K138T (=JCM 33518T=CGMCC 4.7672T), Jiangella aurantiaca 8K307T (=JCM 33519T=CGMCC 4.7621T) and Jiangella ureilytica KC603T (=JCM 33520T=CGMCC 4.7618T).
32228773	13	62	theme	=JCM	1706:1709	arg1	8K307T					1698:1703	Jiangella aurantiaca 8K307T	1677:1703	Jiangella aurantiaca 8K307T (=JCM 33519T=CGMCC 4.7621T)	1677:1731	The type strains of the proposed novel species are as follows: Jiangella asiatica 5K138T (=JCM 33518T=CGMCC 4.7672T), Jiangella aurantiaca 8K307T (=JCM 33519T=CGMCC 4.7621T) and Jiangella ureilytica KC603T (=JCM 33520T=CGMCC 4.7618T).
32228773	4	63	theme	novel	339:343	arg1	actinobacteria					345:358	novel actinobacteria	339:358	novel actinobacteria with biosynthetic potential	339:386	Three isolates, 5K138T, 8K307T and KC603T, with typical morphological characteristics of members of the genus Jiangella were obtained during a study searching for novel actinobacteria with biosynthetic potential from the Karakum Desert.
32228773	1	64	theme	Jiangella	112:120	arg1	sp					133:134	Jiangella aurantiaca sp	112:134	Jiangella aurantiaca sp	112:134	nov., Jiangella aurantiaca sp.
32228773	1	64	theme	Jiangella	112:120	arg1	nov.					106:109	nov.	106:109	nov.	106:109	nov., Jiangella aurantiaca sp.
32228773	6	65	from	acid	654:657	arg1	peptidoglycan					676:688	the cell wall peptidoglycan	662:688	the cell wall peptidoglycan	662:688	The strains showed chemotaxonomic properties consistent with their classification as representing members of the genus Jiangella such as ll-diaminopimelic acid in the cell wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as major polar lipids as well as MK-9(H4) as a major menaquinone.
32228773	7	66	dep	Jiangella	927:935	arg1	45237T					946:951	DSM 45237T	942:951	Jiangella alba DSM 45237T	927:951	Pairwise sequence comparisons of the 16S rRNA genes showed that the strains were closely related to Jiangella alba DSM 45237T, Jiangella rhizosphaerae NEAU-YY265T and Jiangella mangrovi 3SM4-07T with higher than 99 % sequence identities.
32228773	7	66	dep	Jiangella	927:935	arg1	alba					937:940	Jiangella alba DSM 45237T	927:951	Jiangella alba DSM 45237T	927:951	Pairwise sequence comparisons of the 16S rRNA genes showed that the strains were closely related to Jiangella alba DSM 45237T, Jiangella rhizosphaerae NEAU-YY265T and Jiangella mangrovi 3SM4-07T with higher than 99 % sequence identities.
32228773	1	67	theme	aurantiaca	122:131	arg1	sp					133:134	Jiangella aurantiaca sp	112:134	Jiangella aurantiaca sp	112:134	nov., Jiangella aurantiaca sp.
32228773	1	67	theme	aurantiaca	122:131	arg1	nov.					106:109	nov.	106:109	nov.	106:109	nov., Jiangella aurantiaca sp.
32228773	7	68	theme	DSM	942:944	arg1	45237T					946:951	DSM 45237T	942:951	Jiangella alba DSM 45237T	927:951	Pairwise sequence comparisons of the 16S rRNA genes showed that the strains were closely related to Jiangella alba DSM 45237T, Jiangella rhizosphaerae NEAU-YY265T and Jiangella mangrovi 3SM4-07T with higher than 99 % sequence identities.
32228773	9	69	dep	strains	1298:1304	arg1	strains					1298:1304	strains	1298:1304	strains 5K138T, 8K307T and KC603T	1298:1330	Therefore, strains 5K138T, 8K307T and KC603T should each be classified as representing a novel species within the genus Jiangella, for which the names Jiangella asiatica sp.
32228773	9	69	dep	strains	1298:1304	arg1	8K307T					1314:1319	8K307T	1314:1319	8K307T	1314:1319	Therefore, strains 5K138T, 8K307T and KC603T should each be classified as representing a novel species within the genus Jiangella, for which the names Jiangella asiatica sp.
32228773	9	69	dep	strains	1298:1304	arg1	5K138T					1306:1311	5K138T	1306:1311	5K138T	1306:1311	Therefore, strains 5K138T, 8K307T and KC603T should each be classified as representing a novel species within the genus Jiangella, for which the names Jiangella asiatica sp.
32228773	9	69	dep	strains	1298:1304	arg1	KC603T					1325:1330	KC603T	1325:1330	KC603T	1325:1330	Therefore, strains 5K138T, 8K307T and KC603T should each be classified as representing a novel species within the genus Jiangella, for which the names Jiangella asiatica sp.
32228773	8	70	theme	comparative	1154:1164	arg1	analyses					1166:1173	genome-based comparative analyses	1141:1173	a combination of phenotypic and phylogenetic approaches as well as genome-based comparative analyses	1074:1173	However, a combination of phenotypic and phylogenetic approaches as well as genome-based comparative analyses confirmed the taxonomic positions of these strains as representing distinct species within the genus Jiangella.
32228773	9	71	dep	sp	1457:1458	arg1	names					1432:1436	the names	1428:1436	the names	1428:1436	Therefore, strains 5K138T, 8K307T and KC603T should each be classified as representing a novel species within the genus Jiangella, for which the names Jiangella asiatica sp.
32228773	13	72	theme	type	1563:1566	arg1	strains					1568:1574	The type strains	1559:1574	The type strains of the proposed novel species	1559:1604	The type strains of the proposed novel species are as follows: Jiangella asiatica 5K138T (=JCM 33518T=CGMCC 4.7672T), Jiangella aurantiaca 8K307T (=JCM 33519T=CGMCC 4.7621T) and Jiangella ureilytica KC603T (=JCM 33520T=CGMCC 4.7618T).
32228773	11	73	theme	ureilytica	1511:1520	arg1	sp					1522:1523	ureilytica sp	1511:1523	ureilytica sp	1511:1523	nov. and Jiangella ureilytica sp.
32228773	4	74	theme	typical	224:230	arg1	characteristics					246:260	typical morphological characteristics	224:260	typical morphological characteristics of members of the genus Jiangella	224:294	Three isolates, 5K138T, 8K307T and KC603T, with typical morphological characteristics of members of the genus Jiangella were obtained during a study searching for novel actinobacteria with biosynthetic potential from the Karakum Desert.
32228773	7	75	theme	genes	873:877	arg1	comparisons					845:855	Pairwise sequence comparisons	827:855	Pairwise sequence comparisons of the 16S rRNA genes	827:877	Pairwise sequence comparisons of the 16S rRNA genes showed that the strains were closely related to Jiangella alba DSM 45237T, Jiangella rhizosphaerae NEAU-YY265T and Jiangella mangrovi 3SM4-07T with higher than 99 % sequence identities.
32228773	0	76	dep	classification	13:26	arg1	sp					102:103	Jiangella asiatica sp	83:103	Genome-based classification of three novel actinobacteria from the Karakum Desert: Jiangella asiatica sp.	0:104	Genome-based classification of three novel actinobacteria from the Karakum Desert: Jiangella asiatica sp.
32228773	13	77	theme	species	1598:1604	arg1	strains					1568:1574	The type strains	1559:1574	The type strains of the proposed novel species	1559:1604	The type strains of the proposed novel species are as follows: Jiangella asiatica 5K138T (=JCM 33518T=CGMCC 4.7672T), Jiangella aurantiaca 8K307T (=JCM 33519T=CGMCC 4.7621T) and Jiangella ureilytica KC603T (=JCM 33520T=CGMCC 4.7618T).
32228773	6	78	theme	cell	666:669	arg1	peptidoglycan					676:688	the cell wall peptidoglycan	662:688	the cell wall peptidoglycan	662:688	The strains showed chemotaxonomic properties consistent with their classification as representing members of the genus Jiangella such as ll-diaminopimelic acid in the cell wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as major polar lipids as well as MK-9(H4) as a major menaquinone.
32228773	9	79	theme	Jiangella	1438:1446	arg1	sp					1457:1458	Jiangella asiatica sp	1438:1458	Jiangella asiatica sp	1438:1458	Therefore, strains 5K138T, 8K307T and KC603T should each be classified as representing a novel species within the genus Jiangella, for which the names Jiangella asiatica sp.
32228773	0	80	theme	Karakum	67:73	arg1	Desert					75:80	the Karakum Desert	63:80	the Karakum Desert	63:80	Genome-based classification of three novel actinobacteria from the Karakum Desert: Jiangella asiatica sp.
32228773	6	81	with	consistent	544:553	arg1	classification					566:579	their classification	560:579	their classification as representing members of the genus Jiangella such as ll-diaminopimelic acid in the cell wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as major polar lipids as well as MK-9(H4) as a major menaquinone	560:824	The strains showed chemotaxonomic properties consistent with their classification as representing members of the genus Jiangella such as ll-diaminopimelic acid in the cell wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as major polar lipids as well as MK-9(H4) as a major menaquinone.
32228773	9	82	theme	asiatica	1448:1455	arg1	sp					1457:1458	Jiangella asiatica sp	1438:1458	Jiangella asiatica sp	1438:1458	Therefore, strains 5K138T, 8K307T and KC603T should each be classified as representing a novel species within the genus Jiangella, for which the names Jiangella asiatica sp.
32163465	2	0	theme	pathogenic	536:545	arg1	species					547:553	an emerging pathogenic species	524:553	an emerging pathogenic species	524:553	However, Caulobacter species have been implicated as the causative agents of several hospital-acquired infections, raising the question of whether these clinical isolates represent an emerging pathogenic species or whether Caulobacters on whole possess previously-unappreciated virulence capability.
32163465	11	1	from	virulent	2005:2012	arg1	contexts					2026:2033	specific contexts	2017:2033	specific contexts	2017:2033	Taken together, our findings suggest that many Caulobacter species can be virulent in specific contexts and highlight the importance of broadening our methods for identifying and characterizing potential pathogens.
32163465	1	2	theme	bioengineering	261:274	arg1	vector					276:281	a bioengineering vector	259:281	a bioengineering vector for various environmental remediation and medical purposes	259:340	The Caulobacter genus, including the widely-studied model organism Caulobacter crescentus, has been thought to be non-pathogenic and thus proposed as a bioengineering vector for various environmental remediation and medical purposes.
32163465	1	2	theme	bioengineering	261:274	arg1	genus					125:129	The Caulobacter genus	109:129	The Caulobacter genus	109:129	The Caulobacter genus, including the widely-studied model organism Caulobacter crescentus, has been thought to be non-pathogenic and thus proposed as a bioengineering vector for various environmental remediation and medical purposes.
32163465	2	3	theme	emerging	527:534	arg1	species					547:553	an emerging pathogenic species	524:553	an emerging pathogenic species	524:553	However, Caulobacter species have been implicated as the causative agents of several hospital-acquired infections, raising the question of whether these clinical isolates represent an emerging pathogenic species or whether Caulobacters on whole possess previously-unappreciated virulence capability.
32163465	7	4	theme	other	1218:1222	arg1	Caulobacters					1224:1235	other Caulobacters	1218:1235	other Caulobacters	1218:1235	The similarity to other Caulobacters and lack of obvious pathogenesis markers suggested that C. mirare is not unique amongst Caulobacters and that consequently other Caulobacters may also have the potential to be virulent.
32163465	3	5	theme	potential	738:746	arg1	pathogenicity					748:760	the potential pathogenicity	734:760	the potential pathogenicity of this bacterium	734:778	Given the proposed environmental and medical applications for C. crescentus, understanding the potential pathogenicity of this bacterium is crucial.
32163465	10	6	theme	differential	1908:1919	arg1	toxicity					1921:1928	their differential toxicity	1902:1928	their differential toxicity	1902:1928	Further characterization showed that Caulobacter pathogenesis in the Galleria model is mediated by lipopolysaccharide (LPS), and that differences in LPS chemical composition across species could explain their differential toxicity.
32163465	8	7	theme	animal	1515:1520	arg1	host					1522:1525	the model animal host	1505:1525	the model animal host	1505:1525	We tested this hypothesis by characterizing the ability of Caulobacters to infect the model animal host Galleria mellonella.
32163465	8	8	theme	model	1509:1513	arg1	host					1522:1525	the model animal host	1505:1525	the model animal host	1505:1525	We tested this hypothesis by characterizing the ability of Caulobacters to infect the model animal host Galleria mellonella.
32163465	5	9	theme	culture	1052:1058	arg1	conditions					1060:1069	standard clinical culture conditions	1034:1069	standard clinical culture conditions	1034:1069	We found that the clinical isolate represents a new species, Caulobacter mirare that, unlike C. crescentus, grows well in standard clinical culture conditions.
32163465	5	10	theme	clinical	1043:1050	arg1	conditions					1060:1069	standard clinical culture conditions	1034:1069	standard clinical culture conditions	1034:1069	We found that the clinical isolate represents a new species, Caulobacter mirare that, unlike C. crescentus, grows well in standard clinical culture conditions.
32163465	7	11	theme	C.	1293:1294	arg1	mirare					1296:1301	C. mirare	1293:1301	C. mirare	1293:1301	The similarity to other Caulobacters and lack of obvious pathogenesis markers suggested that C. mirare is not unique amongst Caulobacters and that consequently other Caulobacters may also have the potential to be virulent.
32163465	9	12	theme	crescentus	1597:1606	arg1	strains					1583:1589	two different lab strains	1565:1589	two different lab strains of C. crescentus	1565:1606	In this context, two different lab strains of C. crescentus proved to be as pathogenic as C. mirare, while lab strains of E. coli were non-pathogenic.
32163465	11	13	theme	potential	2125:2133	arg1	pathogens					2135:2143	potential pathogens	2125:2143	potential pathogens	2125:2143	Taken together, our findings suggest that many Caulobacter species can be virulent in specific contexts and highlight the importance of broadening our methods for identifying and characterizing potential pathogens.
32163465	2	14	contain	possess	588:594	arg1	Caulobacters					566:577	Caulobacters	566:577	Caulobacters on whole	566:586	However, Caulobacter species have been implicated as the causative agents of several hospital-acquired infections, raising the question of whether these clinical isolates represent an emerging pathogenic species or whether Caulobacters on whole possess previously-unappreciated virulence capability.
32163465	2	14	contain	possess	588:594	arg2	capability					631:640	previously-unappreciated virulence capability	596:640	previously-unappreciated virulence capability	596:640	However, Caulobacter species have been implicated as the causative agents of several hospital-acquired infections, raising the question of whether these clinical isolates represent an emerging pathogenic species or whether Caulobacters on whole possess previously-unappreciated virulence capability.
32163465	1	15	theme	widely-studied	146:159	arg1	organism					167:174	widely-studied model organism	146:174	the widely-studied model organism Caulobacter crescentus	142:197	The Caulobacter genus, including the widely-studied model organism Caulobacter crescentus, has been thought to be non-pathogenic and thus proposed as a bioengineering vector for various environmental remediation and medical purposes.
32163465	0	16	from	virulent	56:63	arg1	model					102:106	the Galleria mellonella infection model	68:106	the Galleria mellonella infection model	68:106	Both clinical and environmental Caulobacter species are virulent in the Galleria mellonella infection model.
32163465	10	17	theme	Galleria	1768:1775	arg1	model					1777:1781	the Galleria model	1764:1781	the Galleria model	1764:1781	Further characterization showed that Caulobacter pathogenesis in the Galleria model is mediated by lipopolysaccharide (LPS), and that differences in LPS chemical composition across species could explain their differential toxicity.
32163465	9	18	theme	C.	1638:1639	arg1	mirare					1641:1646	C. mirare	1638:1646	C. mirare	1638:1646	In this context, two different lab strains of C. crescentus proved to be as pathogenic as C. mirare, while lab strains of E. coli were non-pathogenic.
32163465	2	19	theme	hospital-acquired	428:444	arg1	infections					446:455	several hospital-acquired infections	420:455	several hospital-acquired infections	420:455	However, Caulobacter species have been implicated as the causative agents of several hospital-acquired infections, raising the question of whether these clinical isolates represent an emerging pathogenic species or whether Caulobacters on whole possess previously-unappreciated virulence capability.
32163465	1	20	theme	various	287:293	arg1	remediation					309:319	various environmental remediation	287:319	various environmental remediation	287:319	The Caulobacter genus, including the widely-studied model organism Caulobacter crescentus, has been thought to be non-pathogenic and thus proposed as a bioengineering vector for various environmental remediation and medical purposes.
32163465	7	21	contain	have	1388:1391	arg2	potential					1397:1405	the potential to be virulent	1393:1420	the potential to be virulent	1393:1420	The similarity to other Caulobacters and lack of obvious pathogenesis markers suggested that C. mirare is not unique amongst Caulobacters and that consequently other Caulobacters may also have the potential to be virulent.
32163465	7	21	contain	have	1388:1391	arg1	Caulobacters					1366:1377	consequently other Caulobacters	1347:1377	consequently other Caulobacters	1347:1377	The similarity to other Caulobacters and lack of obvious pathogenesis markers suggested that C. mirare is not unique amongst Caulobacters and that consequently other Caulobacters may also have the potential to be virulent.
32163465	2	22	theme	several	420:426	arg1	infections					446:455	several hospital-acquired infections	420:455	several hospital-acquired infections	420:455	However, Caulobacter species have been implicated as the causative agents of several hospital-acquired infections, raising the question of whether these clinical isolates represent an emerging pathogenic species or whether Caulobacters on whole possess previously-unappreciated virulence capability.
32163465	0	23	theme	clinical	5:12	arg1	Caulobacter species					32:50	Both clinical and environmental Caulobacter species	0:50	Both clinical and environmental Caulobacter species	0:50	Both clinical and environmental Caulobacter species are virulent in the Galleria mellonella infection model.
32163465	0	24	theme	mellonella infection	81:100	arg1	model					102:106	the Galleria mellonella infection model	68:106	the Galleria mellonella infection model	68:106	Both clinical and environmental Caulobacter species are virulent in the Galleria mellonella infection model.
32163465	6	25	theme	C.	1072:1073	arg1	mirare					1075:1080	C. mirare	1072:1080	C. mirare	1072:1080	C. mirare phylogenetically resembles both C. crescentus and the related C. segnis, which was also thought to be non-pathogenic.
32163465	11	26	theme	specific	2017:2024	arg1	contexts					2026:2033	specific contexts	2017:2033	specific contexts	2017:2033	Taken together, our findings suggest that many Caulobacter species can be virulent in specific contexts and highlight the importance of broadening our methods for identifying and characterizing potential pathogens.
32163465	11	27	theme	many	1973:1976	arg1	species					1990:1996	many Caulobacter species	1973:1996	many Caulobacter species	1973:1996	Taken together, our findings suggest that many Caulobacter species can be virulent in specific contexts and highlight the importance of broadening our methods for identifying and characterizing potential pathogens.
32163465	1	28	theme	environmental	295:307	arg1	remediation					309:319	various environmental remediation	287:319	various environmental remediation	287:319	The Caulobacter genus, including the widely-studied model organism Caulobacter crescentus, has been thought to be non-pathogenic and thus proposed as a bioengineering vector for various environmental remediation and medical purposes.
32163465	9	29	theme	lab	1655:1657	arg1	strains					1659:1665	lab strains	1655:1665	lab strains of E. coli	1655:1676	In this context, two different lab strains of C. crescentus proved to be as pathogenic as C. mirare, while lab strains of E. coli were non-pathogenic.
32163465	3	30	theme	bacterium	770:778	arg1	pathogenicity					748:760	the potential pathogenicity	734:760	the potential pathogenicity of this bacterium	734:778	Given the proposed environmental and medical applications for C. crescentus, understanding the potential pathogenicity of this bacterium is crucial.
32163465	5	31	theme	Caulobacter	973:983	arg1	species					964:970	a new species	958:970	a new species	958:970	We found that the clinical isolate represents a new species, Caulobacter mirare that, unlike C. crescentus, grows well in standard clinical culture conditions.
32163465	5	31	theme	Caulobacter	973:983	arg1	mirare					985:990	Caulobacter mirare	973:990	Caulobacter mirare that, unlike C. crescentus, grows well in standard clinical culture conditions	973:1069	We found that the clinical isolate represents a new species, Caulobacter mirare that, unlike C. crescentus, grows well in standard clinical culture conditions.
32163465	0	32	theme	environmental	18:30	arg1	Caulobacter species					32:50	Both clinical and environmental Caulobacter species	0:50	Both clinical and environmental Caulobacter species	0:50	Both clinical and environmental Caulobacter species are virulent in the Galleria mellonella infection model.
32163465	4	33	theme	Caulobacter	830:840	arg1	isolate					842:848	a clinical Caulobacter isolate	819:848	a clinical Caulobacter isolate to determine if it has acquired novel virulence determinants	819:909	Consequently, we sequenced a clinical Caulobacter isolate to determine if it has acquired novel virulence determinants.
32163465	1	34	theme	Caulobacter	113:123	arg1	vector					276:281	a bioengineering vector	259:281	a bioengineering vector for various environmental remediation and medical purposes	259:340	The Caulobacter genus, including the widely-studied model organism Caulobacter crescentus, has been thought to be non-pathogenic and thus proposed as a bioengineering vector for various environmental remediation and medical purposes.
32163465	1	34	theme	Caulobacter	113:123	arg1	genus					125:129	The Caulobacter genus	109:129	The Caulobacter genus	109:129	The Caulobacter genus, including the widely-studied model organism Caulobacter crescentus, has been thought to be non-pathogenic and thus proposed as a bioengineering vector for various environmental remediation and medical purposes.
32163465	5	35	theme	clinical	930:937	arg1	isolate					939:945	the clinical isolate	926:945	the clinical isolate	926:945	We found that the clinical isolate represents a new species, Caulobacter mirare that, unlike C. crescentus, grows well in standard clinical culture conditions.
32163465	3	36	theme	proposed	653:660	arg1	applications					688:699	the proposed environmental and medical applications	649:699	the proposed environmental and medical applications for C. crescentus	649:717	Given the proposed environmental and medical applications for C. crescentus, understanding the potential pathogenicity of this bacterium is crucial.
32163465	2	37	theme	virulence	621:629	arg1	capability					631:640	previously-unappreciated virulence capability	596:640	previously-unappreciated virulence capability	596:640	However, Caulobacter species have been implicated as the causative agents of several hospital-acquired infections, raising the question of whether these clinical isolates represent an emerging pathogenic species or whether Caulobacters on whole possess previously-unappreciated virulence capability.
32163465	0	38	from	model	102:106	arg1	virulent					56:63	virulent	56:63	virulent	56:63	Both clinical and environmental Caulobacter species are virulent in the Galleria mellonella infection model.
32163465	1	39	dep	organism	167:174	arg1	crescentus					188:197	Caulobacter crescentus	176:197	the widely-studied model organism Caulobacter crescentus	142:197	The Caulobacter genus, including the widely-studied model organism Caulobacter crescentus, has been thought to be non-pathogenic and thus proposed as a bioengineering vector for various environmental remediation and medical purposes.
32163465	1	40	theme	model	161:165	arg1	organism					167:174	widely-studied model organism	146:174	the widely-studied model organism Caulobacter crescentus	142:197	The Caulobacter genus, including the widely-studied model organism Caulobacter crescentus, has been thought to be non-pathogenic and thus proposed as a bioengineering vector for various environmental remediation and medical purposes.
32163465	1	41	theme	medical	325:331	arg1	purposes					333:340	medical purposes	325:340	medical purposes	325:340	The Caulobacter genus, including the widely-studied model organism Caulobacter crescentus, has been thought to be non-pathogenic and thus proposed as a bioengineering vector for various environmental remediation and medical purposes.
32163465	2	42	theme	previously-unappreciated	596:619	arg1	capability					631:640	previously-unappreciated virulence capability	596:640	previously-unappreciated virulence capability	596:640	However, Caulobacter species have been implicated as the causative agents of several hospital-acquired infections, raising the question of whether these clinical isolates represent an emerging pathogenic species or whether Caulobacters on whole possess previously-unappreciated virulence capability.
32163465	3	43	theme	medical	680:686	arg1	applications					688:699	the proposed environmental and medical applications	649:699	the proposed environmental and medical applications for C. crescentus	649:717	Given the proposed environmental and medical applications for C. crescentus, understanding the potential pathogenicity of this bacterium is crucial.
32163465	10	44	from	differences	1833:1843	arg1	composition					1861:1871	LPS chemical composition	1848:1871	LPS chemical composition across species	1848:1886	Further characterization showed that Caulobacter pathogenesis in the Galleria model is mediated by lipopolysaccharide (LPS), and that differences in LPS chemical composition across species could explain their differential toxicity.
32163465	9	45	theme	different	1569:1577	arg1	strains					1583:1589	two different lab strains	1565:1589	two different lab strains of C. crescentus	1565:1606	In this context, two different lab strains of C. crescentus proved to be as pathogenic as C. mirare, while lab strains of E. coli were non-pathogenic.
32163465	5	46	theme	new	960:962	arg1	species					964:970	a new species	958:970	a new species	958:970	We found that the clinical isolate represents a new species, Caulobacter mirare that, unlike C. crescentus, grows well in standard clinical culture conditions.
32163465	5	46	theme	new	960:962	arg1	mirare					985:990	Caulobacter mirare	973:990	Caulobacter mirare that, unlike C. crescentus, grows well in standard clinical culture conditions	973:1069	We found that the clinical isolate represents a new species, Caulobacter mirare that, unlike C. crescentus, grows well in standard clinical culture conditions.
32163465	10	47	theme	Further	1699:1705	arg1	characterization					1707:1722	Further characterization	1699:1722	Further characterization	1699:1722	Further characterization showed that Caulobacter pathogenesis in the Galleria model is mediated by lipopolysaccharide (LPS), and that differences in LPS chemical composition across species could explain their differential toxicity.
32163465	9	48	theme	lab	1579:1581	arg1	strains					1583:1589	two different lab strains	1565:1589	two different lab strains of C. crescentus	1565:1606	In this context, two different lab strains of C. crescentus proved to be as pathogenic as C. mirare, while lab strains of E. coli were non-pathogenic.
32163465	7	49	theme	obvious	1249:1255	arg1	markers					1270:1276	obvious pathogenesis markers	1249:1276	obvious pathogenesis markers	1249:1276	The similarity to other Caulobacters and lack of obvious pathogenesis markers suggested that C. mirare is not unique amongst Caulobacters and that consequently other Caulobacters may also have the potential to be virulent.
32163465	2	50	theme	infections	446:455	arg1	agents					410:415	the causative agents	396:415	the causative agents of several hospital-acquired infections	396:455	However, Caulobacter species have been implicated as the causative agents of several hospital-acquired infections, raising the question of whether these clinical isolates represent an emerging pathogenic species or whether Caulobacters on whole possess previously-unappreciated virulence capability.
32163465	2	50	theme	infections	446:455	arg1	species					364:370	Caulobacter species	352:370	Caulobacter species	352:370	However, Caulobacter species have been implicated as the causative agents of several hospital-acquired infections, raising the question of whether these clinical isolates represent an emerging pathogenic species or whether Caulobacters on whole possess previously-unappreciated virulence capability.
32163465	3	51	theme	environmental	662:674	arg1	applications					688:699	the proposed environmental and medical applications	649:699	the proposed environmental and medical applications for C. crescentus	649:717	Given the proposed environmental and medical applications for C. crescentus, understanding the potential pathogenicity of this bacterium is crucial.
32163465	2	52	theme	clinical	496:503	arg1	isolates					505:512	these clinical isolates	490:512	these clinical isolates	490:512	However, Caulobacter species have been implicated as the causative agents of several hospital-acquired infections, raising the question of whether these clinical isolates represent an emerging pathogenic species or whether Caulobacters on whole possess previously-unappreciated virulence capability.
32163465	11	53	theme	Caulobacter	1978:1988	arg1	species					1990:1996	many Caulobacter species	1973:1996	many Caulobacter species	1973:1996	Taken together, our findings suggest that many Caulobacter species can be virulent in specific contexts and highlight the importance of broadening our methods for identifying and characterizing potential pathogens.
32163465	2	54	from	Caulobacters	566:577	arg1	whole					582:586	whole	582:586	whole	582:586	However, Caulobacter species have been implicated as the causative agents of several hospital-acquired infections, raising the question of whether these clinical isolates represent an emerging pathogenic species or whether Caulobacters on whole possess previously-unappreciated virulence capability.
32163465	10	55	from	pathogenesis	1748:1759	arg1	model					1777:1781	the Galleria model	1764:1781	the Galleria model	1764:1781	Further characterization showed that Caulobacter pathogenesis in the Galleria model is mediated by lipopolysaccharide (LPS), and that differences in LPS chemical composition across species could explain their differential toxicity.
32163465	10	56	theme	LPS	1848:1850	arg1	composition					1861:1871	LPS chemical composition	1848:1871	LPS chemical composition across species	1848:1886	Further characterization showed that Caulobacter pathogenesis in the Galleria model is mediated by lipopolysaccharide (LPS), and that differences in LPS chemical composition across species could explain their differential toxicity.
32163465	7	57	theme	other	1360:1364	arg1	Caulobacters					1366:1377	consequently other Caulobacters	1347:1377	consequently other Caulobacters	1347:1377	The similarity to other Caulobacters and lack of obvious pathogenesis markers suggested that C. mirare is not unique amongst Caulobacters and that consequently other Caulobacters may also have the potential to be virulent.
32163465	9	58	theme	coli	1673:1676	arg1	strains					1659:1665	lab strains	1655:1665	lab strains of E. coli	1655:1676	In this context, two different lab strains of C. crescentus proved to be as pathogenic as C. mirare, while lab strains of E. coli were non-pathogenic.
32163465	4	59	theme	virulence	888:896	arg1	determinants					898:909	novel virulence determinants	882:909	novel virulence determinants	882:909	Consequently, we sequenced a clinical Caulobacter isolate to determine if it has acquired novel virulence determinants.
32163465	4	60	theme	clinical	821:828	arg1	isolate					842:848	a clinical Caulobacter isolate	819:848	a clinical Caulobacter isolate to determine if it has acquired novel virulence determinants	819:909	Consequently, we sequenced a clinical Caulobacter isolate to determine if it has acquired novel virulence determinants.
32163465	5	61	theme	standard	1034:1041	arg1	conditions					1060:1069	standard clinical culture conditions	1034:1069	standard clinical culture conditions	1034:1069	We found that the clinical isolate represents a new species, Caulobacter mirare that, unlike C. crescentus, grows well in standard clinical culture conditions.
32163465	4	62	theme	novel	882:886	arg1	determinants					898:909	novel virulence determinants	882:909	novel virulence determinants	882:909	Consequently, we sequenced a clinical Caulobacter isolate to determine if it has acquired novel virulence determinants.
32163465	2	63	theme	Caulobacter	352:362	arg1	agents					410:415	the causative agents	396:415	the causative agents of several hospital-acquired infections	396:455	However, Caulobacter species have been implicated as the causative agents of several hospital-acquired infections, raising the question of whether these clinical isolates represent an emerging pathogenic species or whether Caulobacters on whole possess previously-unappreciated virulence capability.
32163465	2	63	theme	Caulobacter	352:362	arg1	species					364:370	Caulobacter species	352:370	Caulobacter species	352:370	However, Caulobacter species have been implicated as the causative agents of several hospital-acquired infections, raising the question of whether these clinical isolates represent an emerging pathogenic species or whether Caulobacters on whole possess previously-unappreciated virulence capability.
32163465	11	64	from	contexts	2026:2033	arg1	virulent					2005:2012	virulent	2005:2012	virulent	2005:2012	Taken together, our findings suggest that many Caulobacter species can be virulent in specific contexts and highlight the importance of broadening our methods for identifying and characterizing potential pathogens.
32163465	10	65	theme	chemical	1852:1859	arg1	composition					1861:1871	LPS chemical composition	1848:1871	LPS chemical composition across species	1848:1886	Further characterization showed that Caulobacter pathogenesis in the Galleria model is mediated by lipopolysaccharide (LPS), and that differences in LPS chemical composition across species could explain their differential toxicity.
32163465	0	66	theme	Galleria	72:79	arg1	model					102:106	the Galleria mellonella infection model	68:106	the Galleria mellonella infection model	68:106	Both clinical and environmental Caulobacter species are virulent in the Galleria mellonella infection model.
32163465	8	67	theme	Caulobacters	1482:1493	arg1	ability					1471:1477	the ability	1467:1477	the ability of Caulobacters to infect the model animal host Galleria mellonella	1467:1545	We tested this hypothesis by characterizing the ability of Caulobacters to infect the model animal host Galleria mellonella.
32163465	2	68	theme	causative	400:408	arg1	agents					410:415	the causative agents	396:415	the causative agents of several hospital-acquired infections	396:455	However, Caulobacter species have been implicated as the causative agents of several hospital-acquired infections, raising the question of whether these clinical isolates represent an emerging pathogenic species or whether Caulobacters on whole possess previously-unappreciated virulence capability.
32163465	2	68	theme	causative	400:408	arg1	species					364:370	Caulobacter species	352:370	Caulobacter species	352:370	However, Caulobacter species have been implicated as the causative agents of several hospital-acquired infections, raising the question of whether these clinical isolates represent an emerging pathogenic species or whether Caulobacters on whole possess previously-unappreciated virulence capability.
32163465	10	69	theme	Caulobacter	1736:1746	arg1	pathogenesis					1748:1759	Caulobacter pathogenesis	1736:1759	Caulobacter pathogenesis in the Galleria model	1736:1781	Further characterization showed that Caulobacter pathogenesis in the Galleria model is mediated by lipopolysaccharide (LPS), and that differences in LPS chemical composition across species could explain their differential toxicity.
32163465	7	70	theme	pathogenesis	1257:1268	arg1	markers					1270:1276	obvious pathogenesis markers	1249:1276	obvious pathogenesis markers	1249:1276	The similarity to other Caulobacters and lack of obvious pathogenesis markers suggested that C. mirare is not unique amongst Caulobacters and that consequently other Caulobacters may also have the potential to be virulent.
32163465	7	71	theme	markers	1270:1276	arg1	lack					1241:1244	lack	1241:1244	lack of obvious pathogenesis markers	1241:1276	The similarity to other Caulobacters and lack of obvious pathogenesis markers suggested that C. mirare is not unique amongst Caulobacters and that consequently other Caulobacters may also have the potential to be virulent.
32163465	7	71	theme	markers	1270:1276	arg1	similarity					1204:1213	The similarity	1200:1213	The similarity to other Caulobacters	1200:1235	The similarity to other Caulobacters and lack of obvious pathogenesis markers suggested that C. mirare is not unique amongst Caulobacters and that consequently other Caulobacters may also have the potential to be virulent.
32183071	8	0	theme	chondrogenic	1061:1072	arg1	differentiation					1074:1088	the chondrogenic differentiation	1057:1088	the chondrogenic differentiation of hSMSC aggregates	1057:1108	To test whether GM3 affected the chondrogenic differentiation of hSMSC aggregates, we used GM3 treatment during chondrogenic differentiation.
32183071	6	1	dep	GM3	743:745	arg1	the					727:729	the	727:729	the	727:729	In this study, the relationship between the ganglioside GM3 and TGF-β activation, during chondrogenic differentiation, was investigated using an aggregate culture of human synovial membrane-derived mesenchymal stem cells.
32183071	10	2	theme	TGF-β	1453:1457	arg1	signaling					1459:1467	TGF-β signaling	1453:1467	TGF-β signaling via SMAD 2/3	1453:1480	Furthermore, GM3 treatment enhanced TGF-β signaling via SMAD 2/3 during the chondrogenic differentiation of hSMSC aggregates.
32183071	9	3	theme	toluidine	1292:1300	arg1	staining					1323:1330	increased toluidine blue, and safranin O staining	1282:1330	increased toluidine blue, and safranin O staining	1282:1330	The results showed that the group treated with 5 μM GM3 had higher expression of chondrogenic specific markers, increased toluidine blue, and safranin O staining, and increased accumulation of glycosaminoglycans compared with the untreated group.
32183071	8	4	theme	chondrogenic	1140:1151	arg1	differentiation					1153:1167	chondrogenic differentiation	1140:1167	chondrogenic differentiation	1140:1167	To test whether GM3 affected the chondrogenic differentiation of hSMSC aggregates, we used GM3 treatment during chondrogenic differentiation.
32183071	8	5	theme	aggregates	1099:1108	arg1	differentiation					1074:1088	the chondrogenic differentiation	1057:1088	the chondrogenic differentiation of hSMSC aggregates	1057:1108	To test whether GM3 affected the chondrogenic differentiation of hSMSC aggregates, we used GM3 treatment during chondrogenic differentiation.
32183071	9	6	theme	O	1321:1321	arg1	staining					1323:1330	increased toluidine blue, and safranin O staining	1282:1330	increased toluidine blue, and safranin O staining	1282:1330	The results showed that the group treated with 5 μM GM3 had higher expression of chondrogenic specific markers, increased toluidine blue, and safranin O staining, and increased accumulation of glycosaminoglycans compared with the untreated group.
32183071	11	7	theme	cell-based	1641:1650	arg1	cartilage					1662:1670	cell-based articular cartilage	1641:1670	cell-based articular cartilage regeneration in articular cartilage disease	1641:1714	Taken together, our results suggested that GM3 may be useful in developing therapeutic agents for cell-based articular cartilage regeneration in articular cartilage disease.
32183071	4	8	theme	chondrogenic	586:597	arg1	differentiation					599:613	chondrogenic differentiation	586:613	chondrogenic differentiation	586:613	Transforming growth factor-beta (TGF-β) signaling plays a critical role in chondrogenic differentiation.
32183071	8	9	theme	hSMSC	1093:1097	arg1	aggregates					1099:1108	hSMSC aggregates	1093:1108	hSMSC aggregates	1093:1108	To test whether GM3 affected the chondrogenic differentiation of hSMSC aggregates, we used GM3 treatment during chondrogenic differentiation.
32183071	7	10	theme	chondrogenic	978:989	arg1	differentiation					991:1005	the chondrogenic differentiation	974:1005	the chondrogenic differentiation of hSMSC aggregates	974:1025	We showed that the gangliosides GM3 and GD3 were expressed after the chondrogenic differentiation of hSMSC aggregates.
32183071	6	11	link	membrane-derived	868:883	arg1	cells					902:906	human synovial membrane-derived mesenchymal stem cells	853:906	human synovial membrane-derived mesenchymal stem cells	853:906	In this study, the relationship between the ganglioside GM3 and TGF-β activation, during chondrogenic differentiation, was investigated using an aggregate culture of human synovial membrane-derived mesenchymal stem cells.
32183071	3	12	located	localized	386:394	arg1	microdomains					426:437	glycosphingolipid-enriched microdomains	399:437	glycosphingolipid-enriched microdomains on the cell surface	399:457	These molecules are localized in glycosphingolipid-enriched microdomains on the cell surface and are regulated by glycosphingolipid composition.
32183071	3	12	located	localized	386:394	arg2	molecules					372:380	These molecules	366:380	These molecules	366:380	These molecules are localized in glycosphingolipid-enriched microdomains on the cell surface and are regulated by glycosphingolipid composition.
32183071	10	13	theme	hSMSC	1525:1529	arg1	aggregates					1531:1540	hSMSC aggregates	1525:1540	hSMSC aggregates	1525:1540	Furthermore, GM3 treatment enhanced TGF-β signaling via SMAD 2/3 during the chondrogenic differentiation of hSMSC aggregates.
32183071	9	14	theme	5	1217:1217	arg1	μM					1219:1220	μM	1219:1220	μM	1219:1220	The results showed that the group treated with 5 μM GM3 had higher expression of chondrogenic specific markers, increased toluidine blue, and safranin O staining, and increased accumulation of glycosaminoglycans compared with the untreated group.
32183071	9	15	contain	had	1226:1228	arg2	expression					1237:1246	higher expression	1230:1246	higher expression of chondrogenic specific markers, increased toluidine blue, and safranin O staining, and increased accumulation of glycosaminoglycans	1230:1380	The results showed that the group treated with 5 μM GM3 had higher expression of chondrogenic specific markers, increased toluidine blue, and safranin O staining, and increased accumulation of glycosaminoglycans compared with the untreated group.
32183071	9	15	contain	had	1226:1228	arg1	group					1198:1202	the group	1194:1202	the group treated with 5 μM GM3	1194:1224	The results showed that the group treated with 5 μM GM3 had higher expression of chondrogenic specific markers, increased toluidine blue, and safranin O staining, and increased accumulation of glycosaminoglycans compared with the untreated group.
32183071	9	16	theme	safranin	1312:1319	arg1	O					1321:1321	safranin O	1312:1321	safranin O	1312:1321	The results showed that the group treated with 5 μM GM3 had higher expression of chondrogenic specific markers, increased toluidine blue, and safranin O staining, and increased accumulation of glycosaminoglycans compared with the untreated group.
32183071	7	17	theme	hSMSC	1010:1014	arg1	aggregates					1016:1025	hSMSC aggregates	1010:1025	hSMSC aggregates	1010:1025	We showed that the gangliosides GM3 and GD3 were expressed after the chondrogenic differentiation of hSMSC aggregates.
32183071	3	18	theme	glycosphingolipid-enriched	399:424	arg1	microdomains					426:437	glycosphingolipid-enriched microdomains	399:437	glycosphingolipid-enriched microdomains on the cell surface	399:457	These molecules are localized in glycosphingolipid-enriched microdomains on the cell surface and are regulated by glycosphingolipid composition.
32183071	4	19	theme	critical	569:576	arg1	role					578:581	a critical role	567:581	a critical role	567:581	Transforming growth factor-beta (TGF-β) signaling plays a critical role in chondrogenic differentiation.
32183071	9	20	theme	staining	1323:1330	arg1	expression					1237:1246	higher expression	1230:1246	higher expression of chondrogenic specific markers, increased toluidine blue, and safranin O staining, and increased accumulation of glycosaminoglycans	1230:1380	The results showed that the group treated with 5 μM GM3 had higher expression of chondrogenic specific markers, increased toluidine blue, and safranin O staining, and increased accumulation of glycosaminoglycans compared with the untreated group.
32183071	6	21	theme	cells	902:906	arg1	culture					842:848	an aggregate culture	829:848	an aggregate culture of human synovial membrane-derived mesenchymal stem cells	829:906	In this study, the relationship between the ganglioside GM3 and TGF-β activation, during chondrogenic differentiation, was investigated using an aggregate culture of human synovial membrane-derived mesenchymal stem cells.
32183071	1	22	theme	Mesenchymal	95:105	arg1	cells					112:116	Mesenchymal stem cells	95:116	Mesenchymal stem cells	95:116	Mesenchymal stem cells, also known as multipotent stromal progenitor cells, can differentiate into cells of mesodermal lineage.
32183071	6	23	theme	stem	897:900	arg1	cells					902:906	human synovial membrane-derived mesenchymal stem cells	853:906	human synovial membrane-derived mesenchymal stem cells	853:906	In this study, the relationship between the ganglioside GM3 and TGF-β activation, during chondrogenic differentiation, was investigated using an aggregate culture of human synovial membrane-derived mesenchymal stem cells.
32183071	1	24	theme	stem	107:110	arg1	cells					112:116	Mesenchymal stem cells	95:116	Mesenchymal stem cells	95:116	Mesenchymal stem cells, also known as multipotent stromal progenitor cells, can differentiate into cells of mesodermal lineage.
32183071	6	25	theme	chondrogenic	776:787	arg1	differentiation					789:803	chondrogenic differentiation	776:803	chondrogenic differentiation	776:803	In this study, the relationship between the ganglioside GM3 and TGF-β activation, during chondrogenic differentiation, was investigated using an aggregate culture of human synovial membrane-derived mesenchymal stem cells.
32183071	11	26	theme	therapeutic	1618:1628	arg1	agents					1630:1635	therapeutic agents	1618:1635	therapeutic agents for cell-based articular cartilage regeneration in articular cartilage disease	1618:1714	Taken together, our results suggested that GM3 may be useful in developing therapeutic agents for cell-based articular cartilage regeneration in articular cartilage disease.
32183071	0	27	theme	Ganglioside	0:10	arg1	GM3					12:14	Ganglioside GM3	0:14	Ganglioside GM3	0:14	Ganglioside GM3 Up-Regulate Chondrogenic Differentiation by Transform Growth Factor Receptors.
32183071	11	28	theme	cartilage	1698:1706	arg1	disease					1708:1714	articular cartilage disease	1688:1714	articular cartilage disease	1688:1714	Taken together, our results suggested that GM3 may be useful in developing therapeutic agents for cell-based articular cartilage regeneration in articular cartilage disease.
32183071	9	29	theme	untreated	1400:1408	arg1	group					1410:1414	the untreated group	1396:1414	the untreated group	1396:1414	The results showed that the group treated with 5 μM GM3 had higher expression of chondrogenic specific markers, increased toluidine blue, and safranin O staining, and increased accumulation of glycosaminoglycans compared with the untreated group.
32183071	6	30	theme	mesenchymal	885:895	arg1	cells					902:906	human synovial membrane-derived mesenchymal stem cells	853:906	human synovial membrane-derived mesenchymal stem cells	853:906	In this study, the relationship between the ganglioside GM3 and TGF-β activation, during chondrogenic differentiation, was investigated using an aggregate culture of human synovial membrane-derived mesenchymal stem cells.
32183071	9	31	theme	higher	1230:1235	arg1	expression					1237:1246	higher expression	1230:1246	higher expression of chondrogenic specific markers, increased toluidine blue, and safranin O staining, and increased accumulation of glycosaminoglycans	1230:1380	The results showed that the group treated with 5 μM GM3 had higher expression of chondrogenic specific markers, increased toluidine blue, and safranin O staining, and increased accumulation of glycosaminoglycans compared with the untreated group.
32183071	9	32	theme	accumulation	1347:1358	arg1	expression					1237:1246	higher expression	1230:1246	higher expression of chondrogenic specific markers, increased toluidine blue, and safranin O staining, and increased accumulation of glycosaminoglycans	1230:1380	The results showed that the group treated with 5 μM GM3 had higher expression of chondrogenic specific markers, increased toluidine blue, and safranin O staining, and increased accumulation of glycosaminoglycans compared with the untreated group.
32183071	0	33	theme	Chondrogenic	28:39	arg1	Differentiation					41:55	Chondrogenic Differentiation	28:55	Chondrogenic Differentiation	28:55	Ganglioside GM3 Up-Regulate Chondrogenic Differentiation by Transform Growth Factor Receptors.
32183071	9	34	theme	markers	1273:1279	arg1	expression					1237:1246	higher expression	1230:1246	higher expression of chondrogenic specific markers, increased toluidine blue, and safranin O staining, and increased accumulation of glycosaminoglycans	1230:1380	The results showed that the group treated with 5 μM GM3 had higher expression of chondrogenic specific markers, increased toluidine blue, and safranin O staining, and increased accumulation of glycosaminoglycans compared with the untreated group.
32183071	6	35	theme	membrane-derived	868:883	arg1	cells					902:906	human synovial membrane-derived mesenchymal stem cells	853:906	human synovial membrane-derived mesenchymal stem cells	853:906	In this study, the relationship between the ganglioside GM3 and TGF-β activation, during chondrogenic differentiation, was investigated using an aggregate culture of human synovial membrane-derived mesenchymal stem cells.
32183071	2	36	theme	signaling	345:353	arg1	molecules					355:363	several signaling molecules	337:363	several signaling molecules	337:363	Gangliosides are sialic acid-conjugated glycosphingolipids that are believed to regulate cell differentiation and several signaling molecules.
32183071	9	37	theme	blue	1302:1305	arg1	toluidine					1292:1300	toluidine blue	1292:1305	toluidine blue	1292:1305	The results showed that the group treated with 5 μM GM3 had higher expression of chondrogenic specific markers, increased toluidine blue, and safranin O staining, and increased accumulation of glycosaminoglycans compared with the untreated group.
32183071	1	38	theme	mesodermal	203:212	arg1	lineage					214:220	mesodermal lineage	203:220	mesodermal lineage	203:220	Mesenchymal stem cells, also known as multipotent stromal progenitor cells, can differentiate into cells of mesodermal lineage.
32183071	3	39	theme	cell	446:449	arg1	surface					451:457	the cell surface	442:457	the cell surface	442:457	These molecules are localized in glycosphingolipid-enriched microdomains on the cell surface and are regulated by glycosphingolipid composition.
32183071	9	40	theme	chondrogenic	1251:1262	arg1	markers					1273:1279	chondrogenic specific markers	1251:1279	chondrogenic specific markers	1251:1279	The results showed that the group treated with 5 μM GM3 had higher expression of chondrogenic specific markers, increased toluidine blue, and safranin O staining, and increased accumulation of glycosaminoglycans compared with the untreated group.
32183071	9	41	theme	increased	1337:1345	arg1	accumulation					1347:1358	increased accumulation	1337:1358	increased accumulation of glycosaminoglycans	1337:1380	The results showed that the group treated with 5 μM GM3 had higher expression of chondrogenic specific markers, increased toluidine blue, and safranin O staining, and increased accumulation of glycosaminoglycans compared with the untreated group.
32183071	2	42	theme	several	337:343	arg1	molecules					355:363	several signaling molecules	337:363	several signaling molecules	337:363	Gangliosides are sialic acid-conjugated glycosphingolipids that are believed to regulate cell differentiation and several signaling molecules.
32183071	8	43	used	used	1114:1117	arg2	we					1111:1112	we	1111:1112	we	1111:1112	To test whether GM3 affected the chondrogenic differentiation of hSMSC aggregates, we used GM3 treatment during chondrogenic differentiation.
32183071	1	44	theme	lineage	214:220	arg1	cells					194:198	cells	194:198	cells of mesodermal lineage	194:220	Mesenchymal stem cells, also known as multipotent stromal progenitor cells, can differentiate into cells of mesodermal lineage.
32183071	4	45	theme	factor-beta	531:541	arg1	signaling					551:559	Transforming growth factor-beta (TGF-β) signaling	511:559	Transforming growth factor-beta (TGF-β) signaling	511:559	Transforming growth factor-beta (TGF-β) signaling plays a critical role in chondrogenic differentiation.
32183071	6	46	theme	TGF-β	751:755	arg1	activation					757:766	TGF-β activation	751:766	TGF-β activation	751:766	In this study, the relationship between the ganglioside GM3 and TGF-β activation, during chondrogenic differentiation, was investigated using an aggregate culture of human synovial membrane-derived mesenchymal stem cells.
32183071	5	47	from	role	629:632	arg1	chondrogenesis					653:666	chondrogenesis	653:666	chondrogenesis	653:666	However, the role of gangliosides in chondrogenesis is not understood.
32183071	3	48	theme	glycosphingolipid	480:496	arg1	composition					498:508	glycosphingolipid composition	480:508	glycosphingolipid composition	480:508	These molecules are localized in glycosphingolipid-enriched microdomains on the cell surface and are regulated by glycosphingolipid composition.
32183071	3	49	from	microdomains	426:437	arg1	surface					451:457	the cell surface	442:457	the cell surface	442:457	These molecules are localized in glycosphingolipid-enriched microdomains on the cell surface and are regulated by glycosphingolipid composition.
32183071	2	50	theme	acid-conjugated	247:261	arg1	glycosphingolipids					263:280	sialic acid-conjugated glycosphingolipids	240:280	sialic acid-conjugated glycosphingolipids that are believed to regulate cell differentiation and several signaling molecules	240:363	Gangliosides are sialic acid-conjugated glycosphingolipids that are believed to regulate cell differentiation and several signaling molecules.
32183071	2	50	theme	acid-conjugated	247:261	arg1	Gangliosides					223:234	Gangliosides	223:234	Gangliosides	223:234	Gangliosides are sialic acid-conjugated glycosphingolipids that are believed to regulate cell differentiation and several signaling molecules.
32183071	6	51	theme	synovial	859:866	arg1	cells					902:906	human synovial membrane-derived mesenchymal stem cells	853:906	human synovial membrane-derived mesenchymal stem cells	853:906	In this study, the relationship between the ganglioside GM3 and TGF-β activation, during chondrogenic differentiation, was investigated using an aggregate culture of human synovial membrane-derived mesenchymal stem cells.
32183071	0	52	theme	Growth	70:75	arg1	Receptors					84:92	Transform Growth Factor Receptors	60:92	Transform Growth Factor Receptors	60:92	Ganglioside GM3 Up-Regulate Chondrogenic Differentiation by Transform Growth Factor Receptors.
32183071	10	53	theme	GM3	1430:1432	arg1	treatment					1434:1442	GM3 treatment	1430:1442	GM3 treatment	1430:1442	Furthermore, GM3 treatment enhanced TGF-β signaling via SMAD 2/3 during the chondrogenic differentiation of hSMSC aggregates.
32183071	9	54	theme	glycosaminoglycans	1363:1380	arg1	staining					1323:1330	increased toluidine blue, and safranin O staining	1282:1330	increased toluidine blue, and safranin O staining	1282:1330	The results showed that the group treated with 5 μM GM3 had higher expression of chondrogenic specific markers, increased toluidine blue, and safranin O staining, and increased accumulation of glycosaminoglycans compared with the untreated group.
32183071	9	54	theme	glycosaminoglycans	1363:1380	arg1	accumulation					1347:1358	increased accumulation	1337:1358	increased accumulation of glycosaminoglycans	1337:1380	The results showed that the group treated with 5 μM GM3 had higher expression of chondrogenic specific markers, increased toluidine blue, and safranin O staining, and increased accumulation of glycosaminoglycans compared with the untreated group.
32183071	9	54	theme	glycosaminoglycans	1363:1380	arg1	markers					1273:1279	chondrogenic specific markers	1251:1279	chondrogenic specific markers	1251:1279	The results showed that the group treated with 5 μM GM3 had higher expression of chondrogenic specific markers, increased toluidine blue, and safranin O staining, and increased accumulation of glycosaminoglycans compared with the untreated group.
32183071	2	55	theme	sialic	240:245	arg1	glycosphingolipids					263:280	sialic acid-conjugated glycosphingolipids	240:280	sialic acid-conjugated glycosphingolipids that are believed to regulate cell differentiation and several signaling molecules	240:363	Gangliosides are sialic acid-conjugated glycosphingolipids that are believed to regulate cell differentiation and several signaling molecules.
32183071	2	55	theme	sialic	240:245	arg1	Gangliosides					223:234	Gangliosides	223:234	Gangliosides	223:234	Gangliosides are sialic acid-conjugated glycosphingolipids that are believed to regulate cell differentiation and several signaling molecules.
32183071	6	56	theme	human	853:857	arg1	cells					902:906	human synovial membrane-derived mesenchymal stem cells	853:906	human synovial membrane-derived mesenchymal stem cells	853:906	In this study, the relationship between the ganglioside GM3 and TGF-β activation, during chondrogenic differentiation, was investigated using an aggregate culture of human synovial membrane-derived mesenchymal stem cells.
32183071	10	57	theme	aggregates	1531:1540	arg1	differentiation					1506:1520	the chondrogenic differentiation	1489:1520	the chondrogenic differentiation of hSMSC aggregates	1489:1540	Furthermore, GM3 treatment enhanced TGF-β signaling via SMAD 2/3 during the chondrogenic differentiation of hSMSC aggregates.
32183071	2	58	dep	cell	312:315	arg1	differentiation					317:331	differentiation	317:331	differentiation	317:331	Gangliosides are sialic acid-conjugated glycosphingolipids that are believed to regulate cell differentiation and several signaling molecules.
32183071	5	59	theme	gangliosides	637:648	arg1	role					629:632	the role	625:632	the role of gangliosides in chondrogenesis	625:666	However, the role of gangliosides in chondrogenesis is not understood.
32183071	10	60	theme	chondrogenic	1493:1504	arg1	differentiation					1506:1520	the chondrogenic differentiation	1489:1520	the chondrogenic differentiation of hSMSC aggregates	1489:1540	Furthermore, GM3 treatment enhanced TGF-β signaling via SMAD 2/3 during the chondrogenic differentiation of hSMSC aggregates.
32183071	8	61	theme	GM3	1119:1121	arg1	treatment					1123:1131	GM3 treatment	1119:1131	GM3 treatment	1119:1131	To test whether GM3 affected the chondrogenic differentiation of hSMSC aggregates, we used GM3 treatment during chondrogenic differentiation.
32183071	9	62	theme	specific	1264:1271	arg1	markers					1273:1279	chondrogenic specific markers	1251:1279	chondrogenic specific markers	1251:1279	The results showed that the group treated with 5 μM GM3 had higher expression of chondrogenic specific markers, increased toluidine blue, and safranin O staining, and increased accumulation of glycosaminoglycans compared with the untreated group.
32183071	1	63	theme	multipotent	133:143	arg1	cells					164:168	multipotent stromal progenitor cells	133:168	multipotent stromal progenitor cells	133:168	Mesenchymal stem cells, also known as multipotent stromal progenitor cells, can differentiate into cells of mesodermal lineage.
32183071	0	64	dep	Receptors	84:92	arg1	Transform					60:68	Transform	60:68	Transform	60:68	Ganglioside GM3 Up-Regulate Chondrogenic Differentiation by Transform Growth Factor Receptors.
32183071	9	65	theme	μM	1219:1220	arg1	GM3					1222:1224	5 μM GM3	1217:1224	5 μM GM3	1217:1224	The results showed that the group treated with 5 μM GM3 had higher expression of chondrogenic specific markers, increased toluidine blue, and safranin O staining, and increased accumulation of glycosaminoglycans compared with the untreated group.
32183071	4	66	theme	growth	524:529	arg1	TGF-β					544:548	TGF-β	544:548	TGF-β	544:548	Transforming growth factor-beta (TGF-β) signaling plays a critical role in chondrogenic differentiation.
32183071	4	66	theme	growth	524:529	arg1	factor-beta					531:541	Transforming growth factor-beta	511:541	Transforming growth factor-beta (TGF-β) signaling	511:559	Transforming growth factor-beta (TGF-β) signaling plays a critical role in chondrogenic differentiation.
32183071	11	67	from	regeneration	1672:1683	arg1	disease					1708:1714	articular cartilage disease	1688:1714	articular cartilage disease	1688:1714	Taken together, our results suggested that GM3 may be useful in developing therapeutic agents for cell-based articular cartilage regeneration in articular cartilage disease.
32183071	6	68	theme	aggregate	832:840	arg1	culture					842:848	an aggregate culture	829:848	an aggregate culture of human synovial membrane-derived mesenchymal stem cells	829:906	In this study, the relationship between the ganglioside GM3 and TGF-β activation, during chondrogenic differentiation, was investigated using an aggregate culture of human synovial membrane-derived mesenchymal stem cells.
32183071	1	69	theme	stromal	145:151	arg1	cells					164:168	multipotent stromal progenitor cells	133:168	multipotent stromal progenitor cells	133:168	Mesenchymal stem cells, also known as multipotent stromal progenitor cells, can differentiate into cells of mesodermal lineage.
32183071	6	70	theme	ganglioside	731:741	arg1	GM3					743:745	ganglioside GM3	731:745	ganglioside GM3	731:745	In this study, the relationship between the ganglioside GM3 and TGF-β activation, during chondrogenic differentiation, was investigated using an aggregate culture of human synovial membrane-derived mesenchymal stem cells.
32183071	4	71	theme	Transforming	511:522	arg1	TGF-β					544:548	TGF-β	544:548	TGF-β	544:548	Transforming growth factor-beta (TGF-β) signaling plays a critical role in chondrogenic differentiation.
32183071	4	71	theme	Transforming	511:522	arg1	factor-beta					531:541	Transforming growth factor-beta	511:541	Transforming growth factor-beta (TGF-β) signaling	511:559	Transforming growth factor-beta (TGF-β) signaling plays a critical role in chondrogenic differentiation.
32183071	11	72	theme	articular	1652:1660	arg1	cartilage					1662:1670	cell-based articular cartilage	1641:1670	cell-based articular cartilage regeneration in articular cartilage disease	1641:1714	Taken together, our results suggested that GM3 may be useful in developing therapeutic agents for cell-based articular cartilage regeneration in articular cartilage disease.
32183071	7	73	dep	gangliosides	928:939	arg1	GM3					941:943	GM3	941:943	GM3	941:943	We showed that the gangliosides GM3 and GD3 were expressed after the chondrogenic differentiation of hSMSC aggregates.
32183071	7	73	dep	gangliosides	928:939	arg1	gangliosides					928:939	the gangliosides GM3 and GD3	924:951	the gangliosides GM3 and GD3	924:951	We showed that the gangliosides GM3 and GD3 were expressed after the chondrogenic differentiation of hSMSC aggregates.
32183071	7	73	dep	gangliosides	928:939	arg1	GD3					949:951	GD3	949:951	GD3	949:951	We showed that the gangliosides GM3 and GD3 were expressed after the chondrogenic differentiation of hSMSC aggregates.
32183071	1	74	theme	progenitor	153:162	arg1	cells					164:168	multipotent stromal progenitor cells	133:168	multipotent stromal progenitor cells	133:168	Mesenchymal stem cells, also known as multipotent stromal progenitor cells, can differentiate into cells of mesodermal lineage.
32183071	7	75	theme	aggregates	1016:1025	arg1	differentiation					991:1005	the chondrogenic differentiation	974:1005	the chondrogenic differentiation of hSMSC aggregates	974:1025	We showed that the gangliosides GM3 and GD3 were expressed after the chondrogenic differentiation of hSMSC aggregates.
32183071	0	76	theme	Factor	77:82	arg1	Receptors					84:92	Transform Growth Factor Receptors	60:92	Transform Growth Factor Receptors	60:92	Ganglioside GM3 Up-Regulate Chondrogenic Differentiation by Transform Growth Factor Receptors.
32183071	9	77	theme	increased	1282:1290	arg1	staining					1323:1330	increased toluidine blue, and safranin O staining	1282:1330	increased toluidine blue, and safranin O staining	1282:1330	The results showed that the group treated with 5 μM GM3 had higher expression of chondrogenic specific markers, increased toluidine blue, and safranin O staining, and increased accumulation of glycosaminoglycans compared with the untreated group.
32183071	11	78	theme	cartilage	1662:1670	arg1	regeneration					1672:1683	cell-based articular cartilage regeneration	1641:1683	cell-based articular cartilage regeneration in articular cartilage disease	1641:1714	Taken together, our results suggested that GM3 may be useful in developing therapeutic agents for cell-based articular cartilage regeneration in articular cartilage disease.
32183071	11	79	theme	articular	1688:1696	arg1	disease					1708:1714	articular cartilage disease	1688:1714	articular cartilage disease	1688:1714	Taken together, our results suggested that GM3 may be useful in developing therapeutic agents for cell-based articular cartilage regeneration in articular cartilage disease.
32053092	10	0	theme	protaetiae	1702:1711	arg1	sp					1713:1714	the name Microbacterium protaetiae sp	1678:1714	the name Microbacterium protaetiae sp	1678:1714	Based on the polyphasic taxonomic data, strain DFW100M-13T is considered to represent a novel species, for which the name Microbacterium protaetiae sp.
32053092	4	1	contain	had	621:623	arg1	DFW100M-13T					609:619	strain DFW100M-13T	602:619	strain DFW100M-13T	602:619	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	4	1	contain	had	621:623	arg2	similarity					641:650	a high sequence similarity	625:650	a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %),	625:866	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	5	2	theme	rRNA	1063:1066	arg1	tree					1079:1082	the 16S rRNA gene-based tree	1055:1082	the 16S rRNA gene-based tree	1055:1082	A phylogenetic tree based on house-keeping genes also showed the result similar to the 16S rRNA gene-based tree.
32053092	4	3	dep	Microbacterium	773:786	arg1	rhizosphaerae					788:800	rhizosphaerae	788:800	rhizosphaerae	788:800	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	2	4	theme	Gram-stain-positive	101:119	arg1	bacterium					176:184	A Gram-stain-positive, strictly aerobic, polar flagellated, short rod-shaped bacterium	99:184	bacterium	176:184	A Gram-stain-positive, strictly aerobic, polar flagellated, short rod-shaped bacterium, designated DFW100M-13T, was isolated from gut of the larva of Protaetia brevitarsis seulensis collected from Wanju-gun, South Korea.
32053092	10	5	theme	polyphasic	1578:1587	arg1	data					1599:1602	the polyphasic taxonomic data	1574:1602	the polyphasic taxonomic data	1574:1602	Based on the polyphasic taxonomic data, strain DFW100M-13T is considered to represent a novel species, for which the name Microbacterium protaetiae sp.
32053092	7	6	theme	inidentified	1337:1348	arg1	glycolipid					1350:1359	an inidentified glycolipid	1334:1359	an inidentified glycolipid	1334:1359	The polar lipids were composed of diphosphatidylglycerol, phosphatidylglycerol, an inidentified glycolipid and an unidnetified lipid.
32053092	5	7	theme	gene-based	1068:1077	arg1	tree					1079:1082	the 16S rRNA gene-based tree	1055:1082	the 16S rRNA gene-based tree	1055:1082	A phylogenetic tree based on house-keeping genes also showed the result similar to the 16S rRNA gene-based tree.
32053092	3	8	theme	NaCl	340:343	arg1	concentration					345:357	NaCl concentration	340:357	NaCl concentration	340:357	The growth range of NaCl concentration was 0-3 % (w/v) (optimally 0 % (w/v)), the temperature range for growth was 10-40 °C (optimally 28-30 °C), and the pH range for growth was pH 6.0-9.0 (optimally pH 7.0-8.0).
32053092	4	9	theme	Microbacterium	676:689	arg1	members					655:661	members	655:661	members	655:661	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	3	10	dep	%	367:367	arg1	w/v					391:393	w/v	391:393	w/v	391:393	The growth range of NaCl concentration was 0-3 % (w/v) (optimally 0 % (w/v)), the temperature range for growth was 10-40 °C (optimally 28-30 °C), and the pH range for growth was pH 6.0-9.0 (optimally pH 7.0-8.0).
32053092	3	10	dep	%	367:367	arg1	%					388:388	optimally 0 %	376:388	optimally 0 % (w/v)	376:394	The growth range of NaCl concentration was 0-3 % (w/v) (optimally 0 % (w/v)), the temperature range for growth was 10-40 °C (optimally 28-30 °C), and the pH range for growth was pH 6.0-9.0 (optimally pH 7.0-8.0).
32053092	4	11	with	lineage	890:896	arg1	19459T					932:937	Microbacterium luticocti DSM 19459T	903:937	Microbacterium luticocti DSM 19459T	903:937	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	4	12	theme	DSM	928:930	arg1	19459T					932:937	Microbacterium luticocti DSM 19459T	903:937	Microbacterium luticocti DSM 19459T	903:937	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	3	13	theme	concentration	345:357	arg1	%					367:367	0-3 %	363:367	0-3 % (w/v) (optimally 0 % (w/v))	363:395	The growth range of NaCl concentration was 0-3 % (w/v) (optimally 0 % (w/v)), the temperature range for growth was 10-40 °C (optimally 28-30 °C), and the pH range for growth was pH 6.0-9.0 (optimally pH 7.0-8.0).
32053092	3	13	theme	concentration	345:357	arg1	range					331:335	The growth range	320:335	The growth range of NaCl concentration	320:357	The growth range of NaCl concentration was 0-3 % (w/v) (optimally 0 % (w/v)), the temperature range for growth was 10-40 °C (optimally 28-30 °C), and the pH range for growth was pH 6.0-9.0 (optimally pH 7.0-8.0).
32053092	2	14	theme	flagellated	146:156	arg1	bacterium					176:184	A Gram-stain-positive, strictly aerobic, polar flagellated, short rod-shaped bacterium	99:184	bacterium	176:184	A Gram-stain-positive, strictly aerobic, polar flagellated, short rod-shaped bacterium, designated DFW100M-13T, was isolated from gut of the larva of Protaetia brevitarsis seulensis collected from Wanju-gun, South Korea.
32053092	4	15	theme	distinct	881:888	arg1	lineage					890:896	a distinct lineage	879:896	a distinct lineage with Microbacterium luticocti DSM 19459T	879:937	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	8	16	theme	amino	1443:1447	arg1	d-alanine					1455:1463	d-alanine	1455:1463	d-alanine	1455:1463	The peptidoglycan type was supposed to be the B2ß with amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine.
32053092	8	16	theme	amino	1443:1447	arg1	acid					1477:1480	d-glutamic acid	1466:1480	d-glutamic acid	1466:1480	The peptidoglycan type was supposed to be the B2ß with amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine.
32053092	8	16	theme	amino	1443:1447	arg1	d-ornithine					1509:1519	d-ornithine	1509:1519	d-ornithine	1509:1519	The peptidoglycan type was supposed to be the B2ß with amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine.
32053092	8	16	theme	amino	1443:1447	arg1	acids					1449:1453	amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine	1443:1519	amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine	1443:1519	The peptidoglycan type was supposed to be the B2ß with amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine.
32053092	8	16	theme	amino	1443:1447	arg1	glycine					1483:1489	glycine	1483:1489	glycine	1483:1489	The peptidoglycan type was supposed to be the B2ß with amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine.
32053092	8	16	theme	amino	1443:1447	arg1	l-homoserine					1492:1503	l-homoserine	1492:1503	l-homoserine	1492:1503	The peptidoglycan type was supposed to be the B2ß with amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine.
32053092	2	17	attach	isolated	215:222	arg1	gut					229:231	gut	229:231	gut of the larva of Protaetia brevitarsis seulensis collected from Wanju-gun, South Korea	229:317	A Gram-stain-positive, strictly aerobic, polar flagellated, short rod-shaped bacterium, designated DFW100M-13T, was isolated from gut of the larva of Protaetia brevitarsis seulensis collected from Wanju-gun, South Korea.
32053092	2	17	attach	isolated	215:222	arg2	bacterium					176:184	A Gram-stain-positive, strictly aerobic, polar flagellated, short rod-shaped bacterium	99:184	bacterium	176:184	A Gram-stain-positive, strictly aerobic, polar flagellated, short rod-shaped bacterium, designated DFW100M-13T, was isolated from gut of the larva of Protaetia brevitarsis seulensis collected from Wanju-gun, South Korea.
32053092	4	18	theme	SK	851:852	arg1	18T					854:856	Microbacterium immunditiarum SK 18T	822:856	Microbacterium immunditiarum SK 18T (97.0 %)	822:865	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	4	18	theme	SK	851:852	arg1	%					864:864	97.0 %	859:864	97.0 %	859:864	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	7	19	theme	polar	1258:1262	arg1	lipids					1264:1269	The polar lipids	1254:1269	The polar lipids	1254:1269	The polar lipids were composed of diphosphatidylglycerol, phosphatidylglycerol, an inidentified glycolipid and an unidnetified lipid.
32053092	4	20	theme	DSM	752:754	arg1	19459T					756:761	Microbacterium luticocti DSM 19459T	727:761	Microbacterium luticocti DSM 19459T (97.7 %)	727:770	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	4	20	theme	DSM	752:754	arg1	%					769:769	97.7 %	764:769	97.7 %	764:769	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	4	21	theme	sequence	632:639	arg1	similarity					641:650	a high sequence similarity	625:650	a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %),	625:866	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	1	22	theme	larva	57:61	arg1	gut					50:52	gut	50:52	gut of larva of Protaetia brevitarsis seulensis	50:96	nov., isolated from gut of larva of Protaetia brevitarsis seulensis.
32053092	4	23	theme	rRNA	568:571	arg1	sequences					578:586	16S rRNA gene sequences	564:586	16S rRNA gene sequences	564:586	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	7	24	theme	unidnetified	1368:1379	arg1	lipid					1381:1385	an unidnetified lipid	1365:1385	an unidnetified lipid	1365:1385	The polar lipids were composed of diphosphatidylglycerol, phosphatidylglycerol, an inidentified glycolipid and an unidnetified lipid.
32053092	5	25	theme	phylogenetic	974:985	arg1	tree					987:990	A phylogenetic tree	972:990	A phylogenetic tree based on house-keeping genes	972:1019	A phylogenetic tree based on house-keeping genes also showed the result similar to the 16S rRNA gene-based tree.
32053092	2	26	theme	Protaetia	249:257	arg1	larva					240:244	the larva	236:244	the larva of Protaetia brevitarsis seulensis collected from Wanju-gun, South Korea	236:317	A Gram-stain-positive, strictly aerobic, polar flagellated, short rod-shaped bacterium, designated DFW100M-13T, was isolated from gut of the larva of Protaetia brevitarsis seulensis collected from Wanju-gun, South Korea.
32053092	0	27	theme	protaetiae	15:24	arg1	sp					26:27	Microbacterium protaetiae sp	0:27	Microbacterium protaetiae sp.	0:28	Microbacterium protaetiae sp.
32053092	8	28	theme	peptidoglycan	1392:1404	arg1	type					1406:1409	The peptidoglycan type	1388:1409	The peptidoglycan type	1388:1409	The peptidoglycan type was supposed to be the B2ß with amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine.
32053092	8	28	theme	peptidoglycan	1392:1404	arg1	B2ß					1434:1436	the B2ß	1430:1436	the B2ß with amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine	1430:1519	The peptidoglycan type was supposed to be the B2ß with amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine.
32053092	1	29	theme	Protaetia	66:74	arg1	seulensis					88:96	Protaetia brevitarsis seulensis	66:96	Protaetia brevitarsis seulensis	66:96	nov., isolated from gut of larva of Protaetia brevitarsis seulensis.
32053092	9	30	theme	DNA	1534:1536	arg1	%					1562:1562	68.0 mol%	1554:1562	68.0 mol%	1554:1562	The genomic DNA G+C content was 68.0 mol%.
32053092	9	30	theme	DNA	1534:1536	arg1	content					1542:1548	The genomic DNA G+C content	1522:1548	The genomic DNA G+C content	1522:1548	The genomic DNA G+C content was 68.0 mol%.
32053092	3	31	dep	pH	498:499	arg1	7.0-8.0					523:529	pH 7.0-8.0	520:529	optimally pH 7.0-8.0	510:529	The growth range of NaCl concentration was 0-3 % (w/v) (optimally 0 % (w/v)), the temperature range for growth was 10-40 °C (optimally 28-30 °C), and the pH range for growth was pH 6.0-9.0 (optimally pH 7.0-8.0).
32053092	0	32	theme	Microbacterium	0:13	arg1	sp					26:27	Microbacterium protaetiae sp	0:27	Microbacterium protaetiae sp.	0:28	Microbacterium protaetiae sp.
32053092	5	33	theme	similar	1044:1050	arg1	result					1037:1042	the result	1033:1042	the result similar to the 16S rRNA gene-based tree	1033:1082	A phylogenetic tree based on house-keeping genes also showed the result similar to the 16S rRNA gene-based tree.
32053092	6	34	dep	iso-C16 	1204:1211	arg1	 0					1250:1251	 0	1250:1251	 0	1250:1251	The main respiratory quinone (>10 %) was MK-11, MK-12 and MK-10, and the predominant cellular fatty acids (>10 %) were iso-C16 : 0, anteiso-C17 : 0 and anteiso-C15 : 0.
32053092	6	34	dep	iso-C16 	1204:1211	arg1	anteiso-C15 					1237:1248	anteiso-C15 	1237:1248	anteiso-C15 	1237:1248	The main respiratory quinone (>10 %) was MK-11, MK-12 and MK-10, and the predominant cellular fatty acids (>10 %) were iso-C16 : 0, anteiso-C17 : 0 and anteiso-C15 : 0.
32053092	6	34	dep	iso-C16 	1204:1211	arg1	 0					1213:1214	 0	1213:1214	 0	1213:1214	The main respiratory quinone (>10 %) was MK-11, MK-12 and MK-10, and the predominant cellular fatty acids (>10 %) were iso-C16 : 0, anteiso-C17 : 0 and anteiso-C15 : 0.
32053092	6	34	dep	iso-C16 	1204:1211	arg1	 0					1230:1231	 0	1230:1231	 0	1230:1231	The main respiratory quinone (>10 %) was MK-11, MK-12 and MK-10, and the predominant cellular fatty acids (>10 %) were iso-C16 : 0, anteiso-C17 : 0 and anteiso-C15 : 0.
32053092	1	35	theme	brevitarsis	76:86	arg1	seulensis					88:96	Protaetia brevitarsis seulensis	66:96	Protaetia brevitarsis seulensis	66:96	nov., isolated from gut of larva of Protaetia brevitarsis seulensis.
32053092	6	36	theme	cellular	1170:1177	arg1	acids					1185:1189	the predominant cellular fatty acids	1154:1189	the predominant cellular fatty acids (>10 %)	1154:1197	The main respiratory quinone (>10 %) was MK-11, MK-12 and MK-10, and the predominant cellular fatty acids (>10 %) were iso-C16 : 0, anteiso-C17 : 0 and anteiso-C15 : 0.
32053092	6	36	theme	cellular	1170:1177	arg1	%					1196:1196	>10 %	1192:1196	>10 %	1192:1196	The main respiratory quinone (>10 %) was MK-11, MK-12 and MK-10, and the predominant cellular fatty acids (>10 %) were iso-C16 : 0, anteiso-C17 : 0 and anteiso-C15 : 0.
32053092	6	36	theme	cellular	1170:1177	arg1	iso-C16 					1204:1211	iso-C16 	1204:1211	iso-C16 	1204:1211	The main respiratory quinone (>10 %) was MK-11, MK-12 and MK-10, and the predominant cellular fatty acids (>10 %) were iso-C16 : 0, anteiso-C17 : 0 and anteiso-C15 : 0.
32053092	4	37	theme	16S	564:566	arg1	sequences					578:586	16S rRNA gene sequences	564:586	16S rRNA gene sequences	564:586	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	12	38	theme	=KACC	1767:1771	arg1	DFW100M-13T					1754:1764	DFW100M-13T	1754:1764	DFW100M-13T (=KACC 19323T=NBRC 113120T)	1754:1792	The type strain is DFW100M-13T (=KACC 19323T=NBRC 113120T).
32053092	12	38	theme	=KACC	1767:1771	arg1	113120T					1785:1791	=KACC 19323T=NBRC 113120T	1767:1791	=KACC 19323T=NBRC 113120T	1767:1791	The type strain is DFW100M-13T (=KACC 19323T=NBRC 113120T).
32053092	4	39	theme	high	627:630	arg1	similarity					641:650	a high sequence similarity	625:650	a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %),	625:866	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	6	40	theme	fatty	1179:1183	arg1	acids					1185:1189	the predominant cellular fatty acids	1154:1189	the predominant cellular fatty acids (>10 %)	1154:1197	The main respiratory quinone (>10 %) was MK-11, MK-12 and MK-10, and the predominant cellular fatty acids (>10 %) were iso-C16 : 0, anteiso-C17 : 0 and anteiso-C15 : 0.
32053092	6	40	theme	fatty	1179:1183	arg1	%					1196:1196	>10 %	1192:1196	>10 %	1192:1196	The main respiratory quinone (>10 %) was MK-11, MK-12 and MK-10, and the predominant cellular fatty acids (>10 %) were iso-C16 : 0, anteiso-C17 : 0 and anteiso-C15 : 0.
32053092	6	40	theme	fatty	1179:1183	arg1	iso-C16 					1204:1211	iso-C16 	1204:1211	iso-C16 	1204:1211	The main respiratory quinone (>10 %) was MK-11, MK-12 and MK-10, and the predominant cellular fatty acids (>10 %) were iso-C16 : 0, anteiso-C17 : 0 and anteiso-C15 : 0.
32053092	4	41	dep	Microbacterium	903:916	arg1	luticocti					918:926	luticocti	918:926	luticocti	918:926	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	1	42	theme	seulensis	88:96	arg1	larva					57:61	larva	57:61	larva of Protaetia brevitarsis seulensis	57:96	nov., isolated from gut of larva of Protaetia brevitarsis seulensis.
32053092	4	43	dep	Microbacterium	727:740	arg1	luticocti					742:750	luticocti	742:750	luticocti	742:750	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	3	44	dep	10-40 °C	435:442	arg1	10-40 °C					435:442	10-40 °C	435:442	10-40 °C	435:442	The growth range of NaCl concentration was 0-3 % (w/v) (optimally 0 % (w/v)), the temperature range for growth was 10-40 °C (optimally 28-30 °C), and the pH range for growth was pH 6.0-9.0 (optimally pH 7.0-8.0).
32053092	3	44	dep	10-40 °C	435:442	arg1	28-30 °C					455:462	28-30 °C	455:462	28-30 °C	455:462	The growth range of NaCl concentration was 0-3 % (w/v) (optimally 0 % (w/v)), the temperature range for growth was 10-40 °C (optimally 28-30 °C), and the pH range for growth was pH 6.0-9.0 (optimally pH 7.0-8.0).
32053092	3	44	dep	10-40 °C	435:442	arg1	range					414:418	the temperature range	398:418	the temperature range for growth	398:429	The growth range of NaCl concentration was 0-3 % (w/v) (optimally 0 % (w/v)), the temperature range for growth was 10-40 °C (optimally 28-30 °C), and the pH range for growth was pH 6.0-9.0 (optimally pH 7.0-8.0).
32053092	4	45	theme	genus	670:674	arg1	Microbacterium					676:689	the genus Microbacterium	666:689	the genus Microbacterium	666:689	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	3	46	theme	pH	474:475	arg1	pH					498:499	pH 6.0-9.0	498:507	pH 6.0-9.0 (optimally pH 7.0-8.0)	498:530	The growth range of NaCl concentration was 0-3 % (w/v) (optimally 0 % (w/v)), the temperature range for growth was 10-40 °C (optimally 28-30 °C), and the pH range for growth was pH 6.0-9.0 (optimally pH 7.0-8.0).
32053092	3	46	theme	pH	474:475	arg1	range					477:481	the pH range	470:481	the pH range for growth	470:492	The growth range of NaCl concentration was 0-3 % (w/v) (optimally 0 % (w/v)), the temperature range for growth was 10-40 °C (optimally 28-30 °C), and the pH range for growth was pH 6.0-9.0 (optimally pH 7.0-8.0).
32053092	10	47	theme	Microbacterium	1687:1700	arg1	sp					1713:1714	the name Microbacterium protaetiae sp	1678:1714	the name Microbacterium protaetiae sp	1678:1714	Based on the polyphasic taxonomic data, strain DFW100M-13T is considered to represent a novel species, for which the name Microbacterium protaetiae sp.
32053092	5	48	theme	16S	1059:1061	arg1	tree					1079:1082	the 16S rRNA gene-based tree	1055:1082	the 16S rRNA gene-based tree	1055:1082	A phylogenetic tree based on house-keeping genes also showed the result similar to the 16S rRNA gene-based tree.
32053092	4	49	dep	Microbacterium	822:835	arg1	immunditiarum					837:849	immunditiarum	837:849	immunditiarum	837:849	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	2	50	theme	rod-shaped	165:174	arg1	bacterium					176:184	A Gram-stain-positive, strictly aerobic, polar flagellated, short rod-shaped bacterium	99:184	bacterium	176:184	A Gram-stain-positive, strictly aerobic, polar flagellated, short rod-shaped bacterium, designated DFW100M-13T, was isolated from gut of the larva of Protaetia brevitarsis seulensis collected from Wanju-gun, South Korea.
32053092	10	51	theme	strain	1605:1610	arg1	DFW100M-13T					1612:1622	strain DFW100M-13T	1605:1622	strain DFW100M-13T	1605:1622	Based on the polyphasic taxonomic data, strain DFW100M-13T is considered to represent a novel species, for which the name Microbacterium protaetiae sp.
32053092	8	52	theme	d-glutamic	1466:1475	arg1	acid					1477:1480	d-glutamic acid	1466:1480	d-glutamic acid	1466:1480	The peptidoglycan type was supposed to be the B2ß with amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine.
32053092	8	52	theme	d-glutamic	1466:1475	arg1	acids					1449:1453	amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine	1443:1519	amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine	1443:1519	The peptidoglycan type was supposed to be the B2ß with amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine.
32053092	2	53	dep	Gram-stain-positive	101:119	arg1	polar					140:144	polar	140:144	polar	140:144	A Gram-stain-positive, strictly aerobic, polar flagellated, short rod-shaped bacterium, designated DFW100M-13T, was isolated from gut of the larva of Protaetia brevitarsis seulensis collected from Wanju-gun, South Korea.
32053092	2	53	dep	Gram-stain-positive	101:119	arg1	aerobic					131:137	aerobic	131:137	aerobic	131:137	A Gram-stain-positive, strictly aerobic, polar flagellated, short rod-shaped bacterium, designated DFW100M-13T, was isolated from gut of the larva of Protaetia brevitarsis seulensis collected from Wanju-gun, South Korea.
32053092	9	54	theme	68.0 mol	1554:1561	arg1	%					1562:1562	68.0 mol%	1554:1562	68.0 mol%	1554:1562	The genomic DNA G+C content was 68.0 mol%.
32053092	9	54	theme	68.0 mol	1554:1561	arg1	content					1542:1548	The genomic DNA G+C content	1522:1548	The genomic DNA G+C content	1522:1548	The genomic DNA G+C content was 68.0 mol%.
32053092	2	55	theme	short	159:163	arg1	bacterium					176:184	A Gram-stain-positive, strictly aerobic, polar flagellated, short rod-shaped bacterium	99:184	bacterium	176:184	A Gram-stain-positive, strictly aerobic, polar flagellated, short rod-shaped bacterium, designated DFW100M-13T, was isolated from gut of the larva of Protaetia brevitarsis seulensis collected from Wanju-gun, South Korea.
32053092	4	56	theme	highest	703:709	arg1	similarity					711:720	the highest similarity	699:720	the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %)	699:865	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	4	57	theme	Phylogenetic	533:544	arg1	analysis					546:553	Phylogenetic analysis	533:553	Phylogenetic analysis based on 16S rRNA gene sequences	533:586	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	4	58	theme	Microbacterium	822:835	arg1	18T					854:856	Microbacterium immunditiarum SK 18T	822:856	Microbacterium immunditiarum SK 18T (97.0 %)	822:865	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	4	58	theme	Microbacterium	822:835	arg1	%					864:864	97.0 %	859:864	97.0 %	859:864	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	9	59	theme	genomic	1526:1532	arg1	%					1562:1562	68.0 mol%	1554:1562	68.0 mol%	1554:1562	The genomic DNA G+C content was 68.0 mol%.
32053092	9	59	theme	genomic	1526:1532	arg1	content					1542:1548	The genomic DNA G+C content	1522:1548	The genomic DNA G+C content	1522:1548	The genomic DNA G+C content was 68.0 mol%.
32053092	6	60	theme	predominant	1158:1168	arg1	acids					1185:1189	the predominant cellular fatty acids	1154:1189	the predominant cellular fatty acids (>10 %)	1154:1197	The main respiratory quinone (>10 %) was MK-11, MK-12 and MK-10, and the predominant cellular fatty acids (>10 %) were iso-C16 : 0, anteiso-C17 : 0 and anteiso-C15 : 0.
32053092	6	60	theme	predominant	1158:1168	arg1	%					1196:1196	>10 %	1192:1196	>10 %	1192:1196	The main respiratory quinone (>10 %) was MK-11, MK-12 and MK-10, and the predominant cellular fatty acids (>10 %) were iso-C16 : 0, anteiso-C17 : 0 and anteiso-C15 : 0.
32053092	6	60	theme	predominant	1158:1168	arg1	iso-C16 					1204:1211	iso-C16 	1204:1211	iso-C16 	1204:1211	The main respiratory quinone (>10 %) was MK-11, MK-12 and MK-10, and the predominant cellular fatty acids (>10 %) were iso-C16 : 0, anteiso-C17 : 0 and anteiso-C15 : 0.
32053092	4	61	theme	Microbacterium	727:740	arg1	19459T					756:761	Microbacterium luticocti DSM 19459T	727:761	Microbacterium luticocti DSM 19459T (97.7 %)	727:770	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	4	61	theme	Microbacterium	727:740	arg1	%					769:769	97.7 %	764:769	97.7 %	764:769	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	5	62	theme	house-keeping	1001:1013	arg1	genes					1015:1019	house-keeping genes	1001:1019	house-keeping genes	1001:1019	A phylogenetic tree based on house-keeping genes also showed the result similar to the 16S rRNA gene-based tree.
32053092	10	63	theme	name	1682:1685	arg1	sp					1713:1714	the name Microbacterium protaetiae sp	1678:1714	the name Microbacterium protaetiae sp	1678:1714	Based on the polyphasic taxonomic data, strain DFW100M-13T is considered to represent a novel species, for which the name Microbacterium protaetiae sp.
32053092	4	64	theme	Microbacterium	773:786	arg1	CHO1T					802:806	Microbacterium rhizosphaerae CHO1T	773:806	Microbacterium rhizosphaerae CHO1T (97.1 %)	773:815	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	4	64	theme	Microbacterium	773:786	arg1	%					814:814	97.1 %	809:814	97.1 %	809:814	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	10	65	theme	taxonomic	1589:1597	arg1	data					1599:1602	the polyphasic taxonomic data	1574:1602	the polyphasic taxonomic data	1574:1602	Based on the polyphasic taxonomic data, strain DFW100M-13T is considered to represent a novel species, for which the name Microbacterium protaetiae sp.
32053092	4	66	theme	strain	602:607	arg1	DFW100M-13T					609:619	strain DFW100M-13T	602:619	strain DFW100M-13T	602:619	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	8	67	with	B2ß	1434:1436	arg1	d-alanine					1455:1463	d-alanine	1455:1463	d-alanine	1455:1463	The peptidoglycan type was supposed to be the B2ß with amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine.
32053092	8	67	with	B2ß	1434:1436	arg1	acid					1477:1480	d-glutamic acid	1466:1480	d-glutamic acid	1466:1480	The peptidoglycan type was supposed to be the B2ß with amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine.
32053092	8	67	with	B2ß	1434:1436	arg1	d-ornithine					1509:1519	d-ornithine	1509:1519	d-ornithine	1509:1519	The peptidoglycan type was supposed to be the B2ß with amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine.
32053092	8	67	with	B2ß	1434:1436	arg1	acids					1449:1453	amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine	1443:1519	amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine	1443:1519	The peptidoglycan type was supposed to be the B2ß with amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine.
32053092	8	67	with	B2ß	1434:1436	arg1	glycine					1483:1489	glycine	1483:1489	glycine	1483:1489	The peptidoglycan type was supposed to be the B2ß with amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine.
32053092	8	67	with	B2ß	1434:1436	arg1	l-homoserine					1492:1503	l-homoserine	1492:1503	l-homoserine	1492:1503	The peptidoglycan type was supposed to be the B2ß with amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine.
32053092	9	68	theme	G+C	1538:1540	arg1	%					1562:1562	68.0 mol%	1554:1562	68.0 mol%	1554:1562	The genomic DNA G+C content was 68.0 mol%.
32053092	9	68	theme	G+C	1538:1540	arg1	content					1542:1548	The genomic DNA G+C content	1522:1548	The genomic DNA G+C content	1522:1548	The genomic DNA G+C content was 68.0 mol%.
32053092	3	69	theme	pH	520:521	arg1	7.0-8.0					523:529	pH 7.0-8.0	520:529	optimally pH 7.0-8.0	510:529	The growth range of NaCl concentration was 0-3 % (w/v) (optimally 0 % (w/v)), the temperature range for growth was 10-40 °C (optimally 28-30 °C), and the pH range for growth was pH 6.0-9.0 (optimally pH 7.0-8.0).
32053092	6	70	theme	respiratory	1094:1104	arg1	%					1119:1119	>10 %	1115:1119	>10 %	1115:1119	The main respiratory quinone (>10 %) was MK-11, MK-12 and MK-10, and the predominant cellular fatty acids (>10 %) were iso-C16 : 0, anteiso-C17 : 0 and anteiso-C15 : 0.
32053092	6	70	theme	respiratory	1094:1104	arg1	MK-11					1126:1130	MK-11	1126:1130	MK-11	1126:1130	The main respiratory quinone (>10 %) was MK-11, MK-12 and MK-10, and the predominant cellular fatty acids (>10 %) were iso-C16 : 0, anteiso-C17 : 0 and anteiso-C15 : 0.
32053092	6	70	theme	respiratory	1094:1104	arg1	quinone					1106:1112	The main respiratory quinone	1085:1112	The main respiratory quinone (>10 %)	1085:1120	The main respiratory quinone (>10 %) was MK-11, MK-12 and MK-10, and the predominant cellular fatty acids (>10 %) were iso-C16 : 0, anteiso-C17 : 0 and anteiso-C15 : 0.
32053092	3	71	theme	temperature	402:412	arg1	10-40 °C					435:442	10-40 °C	435:442	10-40 °C	435:442	The growth range of NaCl concentration was 0-3 % (w/v) (optimally 0 % (w/v)), the temperature range for growth was 10-40 °C (optimally 28-30 °C), and the pH range for growth was pH 6.0-9.0 (optimally pH 7.0-8.0).
32053092	3	71	theme	temperature	402:412	arg1	28-30 °C					455:462	28-30 °C	455:462	28-30 °C	455:462	The growth range of NaCl concentration was 0-3 % (w/v) (optimally 0 % (w/v)), the temperature range for growth was 10-40 °C (optimally 28-30 °C), and the pH range for growth was pH 6.0-9.0 (optimally pH 7.0-8.0).
32053092	3	71	theme	temperature	402:412	arg1	range					414:418	the temperature range	398:418	the temperature range for growth	398:429	The growth range of NaCl concentration was 0-3 % (w/v) (optimally 0 % (w/v)), the temperature range for growth was 10-40 °C (optimally 28-30 °C), and the pH range for growth was pH 6.0-9.0 (optimally pH 7.0-8.0).
32053092	4	72	contain	having	692:697	arg2	similarity					711:720	the highest similarity	699:720	the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %)	699:865	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	4	72	contain	having	692:697	arg1	members					655:661	members	655:661	members	655:661	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	4	73	theme	Microbacterium	903:916	arg1	19459T					932:937	Microbacterium luticocti DSM 19459T	903:937	Microbacterium luticocti DSM 19459T	903:937	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	2	74	theme	larva	240:244	arg1	gut					229:231	gut	229:231	gut of the larva of Protaetia brevitarsis seulensis collected from Wanju-gun, South Korea	229:317	A Gram-stain-positive, strictly aerobic, polar flagellated, short rod-shaped bacterium, designated DFW100M-13T, was isolated from gut of the larva of Protaetia brevitarsis seulensis collected from Wanju-gun, South Korea.
32053092	4	75	theme	gene	573:576	arg1	sequences					578:586	16S rRNA gene sequences	564:586	16S rRNA gene sequences	564:586	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	10	76	theme	novel	1653:1657	arg1	species					1659:1665	a novel species	1651:1665	a novel species	1651:1665	Based on the polyphasic taxonomic data, strain DFW100M-13T is considered to represent a novel species, for which the name Microbacterium protaetiae sp.
32053092	12	77	theme	type	1739:1742	arg1	DFW100M-13T					1754:1764	DFW100M-13T	1754:1764	DFW100M-13T (=KACC 19323T=NBRC 113120T)	1754:1792	The type strain is DFW100M-13T (=KACC 19323T=NBRC 113120T).
32053092	12	77	theme	type	1739:1742	arg1	strain					1744:1749	The type strain	1735:1749	The type strain	1735:1749	The type strain is DFW100M-13T (=KACC 19323T=NBRC 113120T).
32053092	6	78	theme	main	1089:1092	arg1	%					1119:1119	>10 %	1115:1119	>10 %	1115:1119	The main respiratory quinone (>10 %) was MK-11, MK-12 and MK-10, and the predominant cellular fatty acids (>10 %) were iso-C16 : 0, anteiso-C17 : 0 and anteiso-C15 : 0.
32053092	6	78	theme	main	1089:1092	arg1	MK-11					1126:1130	MK-11	1126:1130	MK-11	1126:1130	The main respiratory quinone (>10 %) was MK-11, MK-12 and MK-10, and the predominant cellular fatty acids (>10 %) were iso-C16 : 0, anteiso-C17 : 0 and anteiso-C15 : 0.
32053092	6	78	theme	main	1089:1092	arg1	quinone					1106:1112	The main respiratory quinone	1085:1112	The main respiratory quinone (>10 %)	1085:1120	The main respiratory quinone (>10 %) was MK-11, MK-12 and MK-10, and the predominant cellular fatty acids (>10 %) were iso-C16 : 0, anteiso-C17 : 0 and anteiso-C15 : 0.
32053092	4	79	theme	genus	950:954	arg1	Microbacterium					956:969	the genus Microbacterium	946:969	the genus Microbacterium	946:969	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	4	80	with	similarity	711:720	arg1	%					814:814	97.1 %	809:814	97.1 %	809:814	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	4	80	with	similarity	711:720	arg1	18T					854:856	Microbacterium immunditiarum SK 18T	822:856	Microbacterium immunditiarum SK 18T (97.0 %)	822:865	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	4	80	with	similarity	711:720	arg1	19459T					756:761	Microbacterium luticocti DSM 19459T	727:761	Microbacterium luticocti DSM 19459T (97.7 %)	727:770	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	4	80	with	similarity	711:720	arg1	%					864:864	97.0 %	859:864	97.0 %	859:864	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	4	80	with	similarity	711:720	arg1	CHO1T					802:806	Microbacterium rhizosphaerae CHO1T	773:806	Microbacterium rhizosphaerae CHO1T (97.1 %)	773:815	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	4	80	with	similarity	711:720	arg1	%					769:769	97.7 %	764:769	97.7 %	764:769	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain DFW100M-13T had a high sequence similarity to members of the genus Microbacterium, having the highest similarity with Microbacterium luticocti DSM 19459T (97.7 %), Microbacterium rhizosphaerae CHO1T (97.1 %), and Microbacterium immunditiarum SK 18T (97.0 %), and formed a distinct lineage with Microbacterium luticocti DSM 19459T within the genus Microbacterium.
32053092	3	81	theme	growth	324:329	arg1	%					367:367	0-3 %	363:367	0-3 % (w/v) (optimally 0 % (w/v))	363:395	The growth range of NaCl concentration was 0-3 % (w/v) (optimally 0 % (w/v)), the temperature range for growth was 10-40 °C (optimally 28-30 °C), and the pH range for growth was pH 6.0-9.0 (optimally pH 7.0-8.0).
32053092	3	81	theme	growth	324:329	arg1	range					331:335	The growth range	320:335	The growth range of NaCl concentration	320:357	The growth range of NaCl concentration was 0-3 % (w/v) (optimally 0 % (w/v)), the temperature range for growth was 10-40 °C (optimally 28-30 °C), and the pH range for growth was pH 6.0-9.0 (optimally pH 7.0-8.0).
32053092	8	82	dep	acids	1449:1453	arg1	d-alanine					1455:1463	d-alanine	1455:1463	d-alanine	1455:1463	The peptidoglycan type was supposed to be the B2ß with amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine.
32053092	8	82	dep	acids	1449:1453	arg1	acid					1477:1480	d-glutamic acid	1466:1480	d-glutamic acid	1466:1480	The peptidoglycan type was supposed to be the B2ß with amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine.
32053092	8	82	dep	acids	1449:1453	arg1	d-ornithine					1509:1519	d-ornithine	1509:1519	d-ornithine	1509:1519	The peptidoglycan type was supposed to be the B2ß with amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine.
32053092	8	82	dep	acids	1449:1453	arg1	acids					1449:1453	amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine	1443:1519	amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine	1443:1519	The peptidoglycan type was supposed to be the B2ß with amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine.
32053092	8	82	dep	acids	1449:1453	arg1	glycine					1483:1489	glycine	1483:1489	glycine	1483:1489	The peptidoglycan type was supposed to be the B2ß with amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine.
32053092	8	82	dep	acids	1449:1453	arg1	l-homoserine					1492:1503	l-homoserine	1492:1503	l-homoserine	1492:1503	The peptidoglycan type was supposed to be the B2ß with amino acids d-alanine, d-glutamic acid, glycine, l-homoserine and d-ornithine.
32053092	2	83	dep	Protaetia	249:257	arg1	seulensis					271:279	Protaetia brevitarsis seulensis	249:279	Protaetia brevitarsis seulensis collected from Wanju-gun, South Korea	249:317	A Gram-stain-positive, strictly aerobic, polar flagellated, short rod-shaped bacterium, designated DFW100M-13T, was isolated from gut of the larva of Protaetia brevitarsis seulensis collected from Wanju-gun, South Korea.
32053092	12	84	theme	19323T=NBRC	1773:1783	arg1	DFW100M-13T					1754:1764	DFW100M-13T	1754:1764	DFW100M-13T (=KACC 19323T=NBRC 113120T)	1754:1792	The type strain is DFW100M-13T (=KACC 19323T=NBRC 113120T).
32053092	12	84	theme	19323T=NBRC	1773:1783	arg1	113120T					1785:1791	=KACC 19323T=NBRC 113120T	1767:1791	=KACC 19323T=NBRC 113120T	1767:1791	The type strain is DFW100M-13T (=KACC 19323T=NBRC 113120T).
31851604	11	0	theme	70.5 mol	1317:1324	arg1	content					1287:1293	The G+C content	1279:1293	The G+C content of the genomic DNA	1279:1312	The G+C content of the genomic DNA is 70.5 mol%.
31851604	11	0	theme	70.5 mol	1317:1324	arg1	%					1325:1325	70.5 mol%	1317:1325	70.5 mol%	1317:1325	The G+C content of the genomic DNA is 70.5 mol%.
31851604	12	1	theme	Agromyces	1483:1491	arg1	sp					1504:1505	the name Agromyces protaetiae sp	1474:1505	the name Agromyces protaetiae sp	1474:1505	On the basis of the genotypic and phenotypic data, we conclude that strain FW100M-8T represents a novel species of the genus Agromyces, for which the name Agromyces protaetiae sp.
31851604	12	2	theme	name	1478:1481	arg1	sp					1504:1505	the name Agromyces protaetiae sp	1474:1505	the name Agromyces protaetiae sp	1474:1505	On the basis of the genotypic and phenotypic data, we conclude that strain FW100M-8T represents a novel species of the genus Agromyces, for which the name Agromyces protaetiae sp.
31851604	2	3	theme	actinobacterial	97:111	arg1	strain					113:118	An actinobacterial strain	94:118	An actinobacterial strain	94:118	An actinobacterial strain, designated FW100M-8T, was isolated from a gut sample of larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, South Korea.
31851604	3	4	dep	forming	364:370	arg1	non-spore					354:362	non-spore	354:362	non-spore	354:362	Cells were Gram-stain-positive, microaerophilic to aerobic, non-spore forming and non-motile.
31851604	4	5	dep	optimum	482:488	arg1	%					493:493	0 %	491:493	0 %	491:493	It grew at pH 7.0-9.0 (optimum, pH 8.0), at 15-35 °C (optimum, 28 °C) and 0-3.0 % (w/v) NaCl (optimum, 0 %).
31851604	12	6	theme	genotypic	1348:1356	arg1	data					1373:1376	the genotypic and phenotypic data	1344:1376	the genotypic and phenotypic data	1344:1376	On the basis of the genotypic and phenotypic data, we conclude that strain FW100M-8T represents a novel species of the genus Agromyces, for which the name Agromyces protaetiae sp.
31851604	5	7	theme	DSM	620:622	arg1	20152T					624:629	DSM 20152T	620:629	DSM 20152T (98.4 %)	620:638	According to the 16S rRNA gene analysis, strain FW100M-8T shared the highest sequence similarity with Agromyces mediolanus DSM 20152T (98.4 %), Agromyces ulmi XIL01T (98.3 %), Agromyces indicus NIO-1018T (98.3 %), Agromyces soli MJ21T (98.3 %), and Agromyces arachidis AK-1T (97.9 %).
31851604	5	7	theme	DSM	620:622	arg1	%					637:637	98.4 %	632:637	98.4 %	632:637	According to the 16S rRNA gene analysis, strain FW100M-8T shared the highest sequence similarity with Agromyces mediolanus DSM 20152T (98.4 %), Agromyces ulmi XIL01T (98.3 %), Agromyces indicus NIO-1018T (98.3 %), Agromyces soli MJ21T (98.3 %), and Agromyces arachidis AK-1T (97.9 %).
31851604	11	8	theme	DNA	1310:1312	arg1	content					1287:1293	The G+C content	1279:1293	The G+C content of the genomic DNA	1279:1312	The G+C content of the genomic DNA is 70.5 mol%.
31851604	11	8	theme	DNA	1310:1312	arg1	%					1325:1325	70.5 mol%	1317:1325	70.5 mol%	1317:1325	The G+C content of the genomic DNA is 70.5 mol%.
31851604	5	9	theme	strain	538:543	arg1	FW100M-8T					545:553	strain FW100M-8T	538:553	strain FW100M-8T	538:553	According to the 16S rRNA gene analysis, strain FW100M-8T shared the highest sequence similarity with Agromyces mediolanus DSM 20152T (98.4 %), Agromyces ulmi XIL01T (98.3 %), Agromyces indicus NIO-1018T (98.3 %), Agromyces soli MJ21T (98.3 %), and Agromyces arachidis AK-1T (97.9 %).
31851604	2	10	from	sample	167:172	arg1	Institute					234:242	the National Institute	221:242	the National Institute of Agricultural Sciences, Wanju-gun, South Korea	221:291	An actinobacterial strain, designated FW100M-8T, was isolated from a gut sample of larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, South Korea.
31851604	12	11	theme	Agromyces	1453:1461	arg1	species					1432:1438	a novel species	1424:1438	a novel species	1424:1438	On the basis of the genotypic and phenotypic data, we conclude that strain FW100M-8T represents a novel species of the genus Agromyces, for which the name Agromyces protaetiae sp.
31851604	12	12	theme	novel	1426:1430	arg1	species					1432:1438	a novel species	1424:1438	a novel species	1424:1438	On the basis of the genotypic and phenotypic data, we conclude that strain FW100M-8T represents a novel species of the genus Agromyces, for which the name Agromyces protaetiae sp.
31851604	6	13	theme	Phylogenetic	782:793	arg1	trees					795:799	Phylogenetic trees	782:799	Phylogenetic trees	782:799	Phylogenetic trees showed that strain FW100M-8T fell into the lineage of the genus Agromyces.
31851604	5	14	dep	shared	555:560	arg1	%					778:778	97.9 %	773:778	97.9 %	773:778	According to the 16S rRNA gene analysis, strain FW100M-8T shared the highest sequence similarity with Agromyces mediolanus DSM 20152T (98.4 %), Agromyces ulmi XIL01T (98.3 %), Agromyces indicus NIO-1018T (98.3 %), Agromyces soli MJ21T (98.3 %), and Agromyces arachidis AK-1T (97.9 %).
31851604	1	15	theme	larva	52:56	arg1	gut					45:47	gut	45:47	gut of larva of Protaetia brevitarsis seulensis	45:91	nov., isolated from gut of larva of Protaetia brevitarsis seulensis.
31851604	5	16	theme	16S	514:516	arg1	analysis					528:535	the 16S rRNA gene analysis	510:535	the 16S rRNA gene analysis	510:535	According to the 16S rRNA gene analysis, strain FW100M-8T shared the highest sequence similarity with Agromyces mediolanus DSM 20152T (98.4 %), Agromyces ulmi XIL01T (98.3 %), Agromyces indicus NIO-1018T (98.3 %), Agromyces soli MJ21T (98.3 %), and Agromyces arachidis AK-1T (97.9 %).
31851604	0	17	theme	protaetiae	10:19	arg1	sp					21:22	Agromyces protaetiae sp	0:22	Agromyces protaetiae sp.	0:23	Agromyces protaetiae sp.
31851604	5	18	theme	rRNA	518:521	arg1	analysis					528:535	the 16S rRNA gene analysis	510:535	the 16S rRNA gene analysis	510:535	According to the 16S rRNA gene analysis, strain FW100M-8T shared the highest sequence similarity with Agromyces mediolanus DSM 20152T (98.4 %), Agromyces ulmi XIL01T (98.3 %), Agromyces indicus NIO-1018T (98.3 %), Agromyces soli MJ21T (98.3 %), and Agromyces arachidis AK-1T (97.9 %).
31851604	12	19	dep	data	1373:1376	arg1	basis					1335:1339	basis	1335:1339	basis	1335:1339	On the basis of the genotypic and phenotypic data, we conclude that strain FW100M-8T represents a novel species of the genus Agromyces, for which the name Agromyces protaetiae sp.
31851604	12	19	dep	data	1373:1376	arg1	the					1331:1333	the	1331:1333	the	1331:1333	On the basis of the genotypic and phenotypic data, we conclude that strain FW100M-8T represents a novel species of the genus Agromyces, for which the name Agromyces protaetiae sp.
31851604	1	20	theme	Protaetia	61:69	arg1	seulensis					83:91	Protaetia brevitarsis seulensis	61:91	Protaetia brevitarsis seulensis	61:91	nov., isolated from gut of larva of Protaetia brevitarsis seulensis.
31851604	6	21	theme	Agromyces	865:873	arg1	lineage					844:850	the lineage	840:850	the lineage of the genus Agromyces	840:873	Phylogenetic trees showed that strain FW100M-8T fell into the lineage of the genus Agromyces.
31851604	0	22	theme	Agromyces	0:8	arg1	sp					21:22	Agromyces protaetiae sp	0:22	Agromyces protaetiae sp.	0:23	Agromyces protaetiae sp.
31851604	5	23	theme	gene	523:526	arg1	analysis					528:535	the 16S rRNA gene analysis	510:535	the 16S rRNA gene analysis	510:535	According to the 16S rRNA gene analysis, strain FW100M-8T shared the highest sequence similarity with Agromyces mediolanus DSM 20152T (98.4 %), Agromyces ulmi XIL01T (98.3 %), Agromyces indicus NIO-1018T (98.3 %), Agromyces soli MJ21T (98.3 %), and Agromyces arachidis AK-1T (97.9 %).
31851604	12	24	theme	strain	1396:1401	arg1	FW100M-8T					1403:1411	strain FW100M-8T	1396:1411	strain FW100M-8T	1396:1411	On the basis of the genotypic and phenotypic data, we conclude that strain FW100M-8T represents a novel species of the genus Agromyces, for which the name Agromyces protaetiae sp.
31851604	2	25	theme	Korea	287:291	arg1	Institute					234:242	the National Institute	221:242	the National Institute of Agricultural Sciences, Wanju-gun, South Korea	221:291	An actinobacterial strain, designated FW100M-8T, was isolated from a gut sample of larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, South Korea.
31851604	11	26	theme	genomic	1302:1308	arg1	DNA					1310:1312	the genomic DNA	1298:1312	the genomic DNA	1298:1312	The G+C content of the genomic DNA is 70.5 mol%.
31851604	6	27	theme	genus	859:863	arg1	Agromyces					865:873	the genus Agromyces	855:873	the genus Agromyces	855:873	Phylogenetic trees showed that strain FW100M-8T fell into the lineage of the genus Agromyces.
31851604	8	28	theme	strain	1026:1031	arg1	FW100M-8T					1033:1041	strain FW100M-8T	1026:1041	strain FW100M-8T	1026:1041	The menaquinones of strain FW100M-8T were MK-12 (46 %), MK-11 (36 %), MK-10 (14 %) and MK-13 (4 %).
31851604	4	29	dep	15-35 °C	432:439	arg1	28 °C					451:455	28 °C	451:455	28 °C	451:455	It grew at pH 7.0-9.0 (optimum, pH 8.0), at 15-35 °C (optimum, 28 °C) and 0-3.0 % (w/v) NaCl (optimum, 0 %).
31851604	4	29	dep	15-35 °C	432:439	arg1	optimum					442:448	optimum	442:448	optimum	442:448	It grew at pH 7.0-9.0 (optimum, pH 8.0), at 15-35 °C (optimum, 28 °C) and 0-3.0 % (w/v) NaCl (optimum, 0 %).
31851604	13	30	theme	strain	1530:1535	arg1	FW100M-8T					1537:1545	strain FW100M-8T	1530:1545	strain FW100M-8T (=KACC 19308T=NBRC 113048T)	1530:1573	nov. is proposed with strain FW100M-8T (=KACC 19308T=NBRC 113048T) as the type strain.
31851604	13	30	theme	strain	1530:1535	arg1	113048T					1566:1572	=KACC 19308T=NBRC 113048T	1548:1572	=KACC 19308T=NBRC 113048T	1548:1572	nov. is proposed with strain FW100M-8T (=KACC 19308T=NBRC 113048T) as the type strain.
31851604	13	31	theme	type	1582:1585	arg1	nov.					1508:1511	nov.	1508:1511	nov.	1508:1511	nov. is proposed with strain FW100M-8T (=KACC 19308T=NBRC 113048T) as the type strain.
31851604	13	31	theme	type	1582:1585	arg1	strain					1587:1592	the type strain	1578:1592	the type strain	1578:1592	nov. is proposed with strain FW100M-8T (=KACC 19308T=NBRC 113048T) as the type strain.
31851604	1	32	theme	seulensis	83:91	arg1	larva					52:56	larva	52:56	larva of Protaetia brevitarsis seulensis	52:91	nov., isolated from gut of larva of Protaetia brevitarsis seulensis.
31851604	1	33	theme	brevitarsis	71:81	arg1	seulensis					83:91	Protaetia brevitarsis seulensis	61:91	Protaetia brevitarsis seulensis	61:91	nov., isolated from gut of larva of Protaetia brevitarsis seulensis.
31851604	2	34	theme	gut	163:165	arg1	sample					167:172	a gut sample	161:172	a gut sample of larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, South Korea	161:291	An actinobacterial strain, designated FW100M-8T, was isolated from a gut sample of larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, South Korea.
31851604	2	35	dep	Protaetia	186:194	arg1	seulensis					208:216	Protaetia brevitarsis seulensis	186:216	Protaetia brevitarsis seulensis	186:216	An actinobacterial strain, designated FW100M-8T, was isolated from a gut sample of larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, South Korea.
31851604	9	36	dep	anteiso-C15 	1133:1144	arg1	 0					1179:1180	 0	1179:1180	 0	1179:1180	The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
31851604	9	36	dep	anteiso-C15 	1133:1144	arg1	iso-C16 					1170:1177	iso-C16 	1170:1177	iso-C16 	1170:1177	The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
31851604	9	36	dep	anteiso-C15 	1133:1144	arg1	 0					1163:1164	 0	1163:1164	 0	1163:1164	The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
31851604	9	36	dep	anteiso-C15 	1133:1144	arg1	 0					1146:1147	 0	1146:1147	 0	1146:1147	The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
31851604	2	37	from	Institute	234:242	arg1	larva					177:181	larva	177:181	larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, South Korea	177:291	An actinobacterial strain, designated FW100M-8T, was isolated from a gut sample of larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, South Korea.
31851604	2	37	from	Institute	234:242	arg1	sample					167:172	a gut sample	161:172	a gut sample of larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, South Korea	161:291	An actinobacterial strain, designated FW100M-8T, was isolated from a gut sample of larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, South Korea.
31851604	2	38	attach	isolated	147:154	arg1	sample					167:172	a gut sample	161:172	a gut sample of larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, South Korea	161:291	An actinobacterial strain, designated FW100M-8T, was isolated from a gut sample of larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, South Korea.
31851604	2	38	attach	isolated	147:154	arg2	strain					113:118	An actinobacterial strain	94:118	An actinobacterial strain	94:118	An actinobacterial strain, designated FW100M-8T, was isolated from a gut sample of larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, South Korea.
31851604	8	39	theme	FW100M-8T	1033:1041	arg1	MK-12					1048:1052	MK-12	1048:1052	MK-12	1048:1052	The menaquinones of strain FW100M-8T were MK-12 (46 %), MK-11 (36 %), MK-10 (14 %) and MK-13 (4 %).
31851604	8	39	theme	FW100M-8T	1033:1041	arg1	menaquinones					1010:1021	The menaquinones	1006:1021	The menaquinones of strain FW100M-8T	1006:1041	The menaquinones of strain FW100M-8T were MK-12 (46 %), MK-11 (36 %), MK-10 (14 %) and MK-13 (4 %).
31851604	10	40	theme	peptidoglycan	1187:1199	arg1	B1					1234:1235	the type B1	1225:1235	the type B1	1225:1235	The peptidoglycan type was supposed to be the type B1, comprising d-Ala, d-Glu, Gly and l-A2bu.
31851604	10	40	theme	peptidoglycan	1187:1199	arg1	type					1201:1204	The peptidoglycan type	1183:1204	The peptidoglycan type	1183:1204	The peptidoglycan type was supposed to be the type B1, comprising d-Ala, d-Glu, Gly and l-A2bu.
31851604	9	41	theme	major	1110:1114	arg1	anteiso-C15 					1133:1144	anteiso-C15 	1133:1144	anteiso-C15 	1133:1144	The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
31851604	9	41	theme	major	1110:1114	arg1	acids					1122:1126	The major fatty acids	1106:1126	The major fatty acids	1106:1126	The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
31851604	10	42	theme	type	1229:1232	arg1	B1					1234:1235	the type B1	1225:1235	the type B1	1225:1235	The peptidoglycan type was supposed to be the type B1, comprising d-Ala, d-Glu, Gly and l-A2bu.
31851604	10	42	theme	type	1229:1232	arg1	type					1201:1204	The peptidoglycan type	1183:1204	The peptidoglycan type	1183:1204	The peptidoglycan type was supposed to be the type B1, comprising d-Ala, d-Glu, Gly and l-A2bu.
31851604	4	43	theme	w/v	471:473	arg1	optimum					482:488	optimum	482:488	optimum	482:488	It grew at pH 7.0-9.0 (optimum, pH 8.0), at 15-35 °C (optimum, 28 °C) and 0-3.0 % (w/v) NaCl (optimum, 0 %).
31851604	4	43	theme	w/v	471:473	arg1	NaCl					476:479	0-3.0 % (w/v) NaCl	462:479	0-3.0 % (w/v) NaCl (optimum, 0 %)	462:494	It grew at pH 7.0-9.0 (optimum, pH 8.0), at 15-35 °C (optimum, 28 °C) and 0-3.0 % (w/v) NaCl (optimum, 0 %).
31851604	12	44	theme	protaetiae	1493:1502	arg1	sp					1504:1505	the name Agromyces protaetiae sp	1474:1505	the name Agromyces protaetiae sp	1474:1505	On the basis of the genotypic and phenotypic data, we conclude that strain FW100M-8T represents a novel species of the genus Agromyces, for which the name Agromyces protaetiae sp.
31851604	7	45	theme	unidentified	955:966	arg1	glycolipid					968:977	an unidentified glycolipid	952:977	an unidentified glycolipid	952:977	The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid and an unidentified lipid.
31851604	7	46	theme	polar	880:884	arg1	lipids					886:891	The polar lipids	876:891	The polar lipids	876:891	The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid and an unidentified lipid.
31851604	2	47	theme	Protaetia	186:194	arg1	larva					177:181	larva	177:181	larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, South Korea	177:291	An actinobacterial strain, designated FW100M-8T, was isolated from a gut sample of larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, South Korea.
31851604	12	48	theme	phenotypic	1362:1371	arg1	data					1373:1376	the genotypic and phenotypic data	1344:1376	the genotypic and phenotypic data	1344:1376	On the basis of the genotypic and phenotypic data, we conclude that strain FW100M-8T represents a novel species of the genus Agromyces, for which the name Agromyces protaetiae sp.
31851604	4	49	theme	%	468:468	arg1	optimum					482:488	optimum	482:488	optimum	482:488	It grew at pH 7.0-9.0 (optimum, pH 8.0), at 15-35 °C (optimum, 28 °C) and 0-3.0 % (w/v) NaCl (optimum, 0 %).
31851604	4	49	theme	%	468:468	arg1	NaCl					476:479	0-3.0 % (w/v) NaCl	462:479	0-3.0 % (w/v) NaCl (optimum, 0 %)	462:494	It grew at pH 7.0-9.0 (optimum, pH 8.0), at 15-35 °C (optimum, 28 °C) and 0-3.0 % (w/v) NaCl (optimum, 0 %).
31851604	5	50	theme	highest	566:572	arg1	similarity					583:592	the highest sequence similarity	562:592	the highest sequence similarity	562:592	According to the 16S rRNA gene analysis, strain FW100M-8T shared the highest sequence similarity with Agromyces mediolanus DSM 20152T (98.4 %), Agromyces ulmi XIL01T (98.3 %), Agromyces indicus NIO-1018T (98.3 %), Agromyces soli MJ21T (98.3 %), and Agromyces arachidis AK-1T (97.9 %).
31851604	2	51	theme	larva	177:181	arg1	sample					167:172	a gut sample	161:172	a gut sample of larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, South Korea	161:291	An actinobacterial strain, designated FW100M-8T, was isolated from a gut sample of larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, South Korea.
31851604	12	52	theme	genus	1447:1451	arg1	Agromyces					1453:1461	the genus Agromyces	1443:1461	the genus Agromyces	1443:1461	On the basis of the genotypic and phenotypic data, we conclude that strain FW100M-8T represents a novel species of the genus Agromyces, for which the name Agromyces protaetiae sp.
31851604	4	53	dep	optimum	411:417	arg1	pH					420:421	pH 8.0	420:425	pH 8.0	420:425	It grew at pH 7.0-9.0 (optimum, pH 8.0), at 15-35 °C (optimum, 28 °C) and 0-3.0 % (w/v) NaCl (optimum, 0 %).
31851604	5	54	theme	sequence	574:581	arg1	similarity					583:592	the highest sequence similarity	562:592	the highest sequence similarity	562:592	According to the 16S rRNA gene analysis, strain FW100M-8T shared the highest sequence similarity with Agromyces mediolanus DSM 20152T (98.4 %), Agromyces ulmi XIL01T (98.3 %), Agromyces indicus NIO-1018T (98.3 %), Agromyces soli MJ21T (98.3 %), and Agromyces arachidis AK-1T (97.9 %).
31851604	13	55	theme	19308T=NBRC	1554:1564	arg1	FW100M-8T					1537:1545	strain FW100M-8T	1530:1545	strain FW100M-8T (=KACC 19308T=NBRC 113048T)	1530:1573	nov. is proposed with strain FW100M-8T (=KACC 19308T=NBRC 113048T) as the type strain.
31851604	13	55	theme	19308T=NBRC	1554:1564	arg1	113048T					1566:1572	=KACC 19308T=NBRC 113048T	1548:1572	=KACC 19308T=NBRC 113048T	1548:1572	nov. is proposed with strain FW100M-8T (=KACC 19308T=NBRC 113048T) as the type strain.
31851604	5	56	dep	Agromyces	599:607	arg1	20152T					624:629	DSM 20152T	620:629	DSM 20152T (98.4 %)	620:638	According to the 16S rRNA gene analysis, strain FW100M-8T shared the highest sequence similarity with Agromyces mediolanus DSM 20152T (98.4 %), Agromyces ulmi XIL01T (98.3 %), Agromyces indicus NIO-1018T (98.3 %), Agromyces soli MJ21T (98.3 %), and Agromyces arachidis AK-1T (97.9 %).
31851604	5	56	dep	Agromyces	599:607	arg1	%					637:637	98.4 %	632:637	98.4 %	632:637	According to the 16S rRNA gene analysis, strain FW100M-8T shared the highest sequence similarity with Agromyces mediolanus DSM 20152T (98.4 %), Agromyces ulmi XIL01T (98.3 %), Agromyces indicus NIO-1018T (98.3 %), Agromyces soli MJ21T (98.3 %), and Agromyces arachidis AK-1T (97.9 %).
31851604	5	56	dep	Agromyces	599:607	arg1	mediolanus					609:618	Agromyces mediolanus DSM 20152T (98.4 %), Agromyces ulmi XIL01T (98.3 %), Agromyces indicus NIO-1018T (98.3 %), Agromyces soli MJ21T (98.3 %), and Agromyces arachidis AK-1T	599:770	mediolanus	609:618	According to the 16S rRNA gene analysis, strain FW100M-8T shared the highest sequence similarity with Agromyces mediolanus DSM 20152T (98.4 %), Agromyces ulmi XIL01T (98.3 %), Agromyces indicus NIO-1018T (98.3 %), Agromyces soli MJ21T (98.3 %), and Agromyces arachidis AK-1T (97.9 %).
31851604	2	57	theme	National	225:232	arg1	Institute					234:242	the National Institute	221:242	the National Institute of Agricultural Sciences, Wanju-gun, South Korea	221:291	An actinobacterial strain, designated FW100M-8T, was isolated from a gut sample of larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, South Korea.
31851604	13	58	theme	=KACC	1548:1552	arg1	FW100M-8T					1537:1545	strain FW100M-8T	1530:1545	strain FW100M-8T (=KACC 19308T=NBRC 113048T)	1530:1573	nov. is proposed with strain FW100M-8T (=KACC 19308T=NBRC 113048T) as the type strain.
31851604	13	58	theme	=KACC	1548:1552	arg1	113048T					1566:1572	=KACC 19308T=NBRC 113048T	1548:1572	=KACC 19308T=NBRC 113048T	1548:1572	nov. is proposed with strain FW100M-8T (=KACC 19308T=NBRC 113048T) as the type strain.
31851604	7	59	theme	unidentified	986:997	arg1	lipid					999:1003	an unidentified lipid	983:1003	an unidentified lipid	983:1003	The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid and an unidentified lipid.
31851604	11	60	theme	G+C	1283:1285	arg1	content					1287:1293	The G+C content	1279:1293	The G+C content of the genomic DNA	1279:1312	The G+C content of the genomic DNA is 70.5 mol%.
31851604	11	60	theme	G+C	1283:1285	arg1	%					1325:1325	70.5 mol%	1317:1325	70.5 mol%	1317:1325	The G+C content of the genomic DNA is 70.5 mol%.
31851604	9	61	theme	fatty	1116:1120	arg1	anteiso-C15 					1133:1144	anteiso-C15 	1133:1144	anteiso-C15 	1133:1144	The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
31851604	9	61	theme	fatty	1116:1120	arg1	acids					1122:1126	The major fatty acids	1106:1126	The major fatty acids	1106:1126	The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
31851604	6	62	theme	strain	813:818	arg1	FW100M-8T					820:828	strain FW100M-8T	813:828	strain FW100M-8T	813:828	Phylogenetic trees showed that strain FW100M-8T fell into the lineage of the genus Agromyces.
31738158	10	0	theme	new	1545:1547	arg1	species					1549:1555	three new species	1539:1555	three new species	1539:1555	Taken together, our results supported the proposition and description of three new species, i.e. Tsukamurella sputi HKU70T (=JCM 33387T=DSM 109106T) sp.
31738158	7	1	theme	nucleotide	1169:1178	arg1	identity					1180:1187	86.3-88.9 % average nucleotide identity	1149:1187	86.3-88.9 % average nucleotide identity	1149:1187	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	6	2	theme	closest	837:843	arg1	species					858:864	their corresponding closest Tsukamurella species	817:864	their corresponding closest Tsukamurella species	817:864	16S rRNA, secA, rpoB and groEL gene sequence analyses revealed that the three strains shared 99.6-99.9, 94.5-96.8, 95.7-97.8 and 97.7-98.9 % nucleotide identities with their corresponding closest Tsukamurella species respectively.
31738158	10	3	theme	HKU70T	1582:1587	arg1	sp					1615:1616	Tsukamurella sputi HKU70T (=JCM 33387T=DSM 109106T) sp	1563:1616	Tsukamurella sputi HKU70T (=JCM 33387T=DSM 109106T) sp	1563:1616	Taken together, our results supported the proposition and description of three new species, i.e. Tsukamurella sputi HKU70T (=JCM 33387T=DSM 109106T) sp.
31738158	7	4	theme	%	1159:1159	arg1	identity					1180:1187	86.3-88.9 % average nucleotide identity	1149:1187	86.3-88.9 % average nucleotide identity	1149:1187	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	9	5	theme	mol	1446:1448	arg1	%					1449:1449	69.9, 70.2 and 70.5 mol%	1426:1449	%	1449:1449	The G+C content determined based on the genome sequence of strains HKU70T, HKU71T and HKU72T were 69.9, 70.2 and 70.5 mol%, respectively.
31738158	9	5	theme	mol	1446:1448	arg1	content					1336:1342	The G+C content	1328:1342	The G+C content determined based on the genome sequence of strains HKU70T, HKU71T and HKU72T	1328:1419	The G+C content determined based on the genome sequence of strains HKU70T, HKU71T and HKU72T were 69.9, 70.2 and 70.5 mol%, respectively.
31738158	8	6	theme	mycolic	1211:1217	arg1	acids					1219:1223	mycolic acids	1211:1223	mycolic acids	1211:1223	Fatty acids, mycolic acids, cell-wall sugars and peptidoglycan analyses showed that they were typical of members of Tsukamurella.
31738158	10	7	theme	Tsukamurella	1563:1574	arg1	109106T					1606:1612	=JCM 33387T=DSM 109106T	1590:1612	=JCM 33387T=DSM 109106T	1590:1612	Taken together, our results supported the proposition and description of three new species, i.e. Tsukamurella sputi HKU70T (=JCM 33387T=DSM 109106T) sp.
31738158	10	7	theme	Tsukamurella	1563:1574	arg1	HKU70T					1582:1587	Tsukamurella sputi HKU70T	1563:1587	Tsukamurella sputi HKU70T (=JCM 33387T=DSM 109106T) sp	1563:1616	Taken together, our results supported the proposition and description of three new species, i.e. Tsukamurella sputi HKU70T (=JCM 33387T=DSM 109106T) sp.
31738158	11	8	theme	=JCM	1662:1665	arg1	HKU71T					1654:1659	HKU71T	1654:1659	HKU71T	1654:1659	nov., Tsukamurella asaccharolytica HKU71T (=JCM 33388T=DSM 109107T) sp.
31738158	11	8	theme	=JCM	1662:1665	arg1	109107T					1678:1684	=JCM 33388T=DSM 109107T	1662:1684	=JCM 33388T=DSM 109107T	1662:1684	nov., Tsukamurella asaccharolytica HKU71T (=JCM 33388T=DSM 109107T) sp.
31738158	7	9	theme	DNA-DNA	880:886	arg1	hybridization					888:900	DNA-DNA hybridization	880:900	DNA-DNA hybridization	880:900	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	12	10	theme	=JCM	1738:1741	arg1	109108T					1754:1760	=JCM 33389T=DSM 109108T	1738:1760	=JCM 33389T=DSM 109108T	1738:1760	nov. and Tsukamurella conjunctivitidis HKU72T (=JCM 33389T=DSM 109108T) sp.
31738158	12	10	theme	=JCM	1738:1741	arg1	nov.					1691:1694	nov.	1691:1694	nov.	1691:1694	nov. and Tsukamurella conjunctivitidis HKU72T (=JCM 33389T=DSM 109108T) sp.
31738158	7	11	theme	Distance	1125:1132	arg1	Calculator					1134:1143	32.2-40.9 % Genome-to-Genome Distance Calculator	1096:1143	32.2-40.9 % Genome-to-Genome Distance Calculator	1096:1143	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	10	12	dep	sp	1615:1616	arg1	i.e.					1558:1561	i.e.	1558:1561	i.e.	1558:1561	Taken together, our results supported the proposition and description of three new species, i.e. Tsukamurella sputi HKU70T (=JCM 33387T=DSM 109106T) sp.
31738158	8	13	theme	members	1303:1309	arg1	typical					1292:1298	typical	1292:1298	typical	1292:1298	Fatty acids, mycolic acids, cell-wall sugars and peptidoglycan analyses showed that they were typical of members of Tsukamurella.
31738158	10	14	theme	=JCM	1590:1593	arg1	109106T					1606:1612	=JCM 33387T=DSM 109106T	1590:1612	=JCM 33387T=DSM 109106T	1590:1612	Taken together, our results supported the proposition and description of three new species, i.e. Tsukamurella sputi HKU70T (=JCM 33387T=DSM 109106T) sp.
31738158	10	14	theme	=JCM	1590:1593	arg1	HKU70T					1582:1587	Tsukamurella sputi HKU70T	1563:1587	Tsukamurella sputi HKU70T (=JCM 33387T=DSM 109106T) sp	1563:1616	Taken together, our results supported the proposition and description of three new species, i.e. Tsukamurella sputi HKU70T (=JCM 33387T=DSM 109106T) sp.
31738158	7	15	theme	%	1106:1106	arg1	Calculator					1134:1143	32.2-40.9 % Genome-to-Genome Distance Calculator	1096:1143	32.2-40.9 % Genome-to-Genome Distance Calculator	1096:1143	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	5	16	theme	aerobic	455:461	arg1	strains					442:448	The three strains	432:448	The three strains	432:448	The three strains were aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from currently recognized Tsukamurella species.
31738158	5	16	theme	aerobic	455:461	arg1	bacilli					535:541	aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli	455:541	aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli	455:541	The three strains were aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from currently recognized Tsukamurella species.
31738158	8	17	theme	peptidoglycan	1247:1259	arg1	analyses					1261:1268	peptidoglycan analyses	1247:1268	peptidoglycan analyses	1247:1268	Fatty acids, mycolic acids, cell-wall sugars and peptidoglycan analyses showed that they were typical of members of Tsukamurella.
31738158	8	18	theme	Tsukamurella	1314:1325	arg1	members					1303:1309	members	1303:1309	members of Tsukamurella	1303:1325	Fatty acids, mycolic acids, cell-wall sugars and peptidoglycan analyses showed that they were typical of members of Tsukamurella.
31738158	2	19	theme	sputi	95:99	arg1	sp					101:102	sputi sp	95:102	sputi sp	95:102	nov. and Tsukamurella sputi sp.
31738158	7	20	theme	genome-to-genome	1067:1082	arg1	comparison					1084:1093	in silico genome-to-genome comparison	1057:1093	in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values]	1057:1195	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	12	21	theme	conjunctivitidis	1713:1728	arg1	HKU72T					1730:1735	Tsukamurella conjunctivitidis HKU72T	1700:1735	Tsukamurella conjunctivitidis HKU72T	1700:1735	nov. and Tsukamurella conjunctivitidis HKU72T (=JCM 33389T=DSM 109108T) sp.
31738158	6	22	dep	rRNA	653:656	arg1	analyses					694:701	gene sequence analyses	680:701	gene sequence analyses	680:701	16S rRNA, secA, rpoB and groEL gene sequence analyses revealed that the three strains shared 99.6-99.9, 94.5-96.8, 95.7-97.8 and 97.7-98.9 % nucleotide identities with their corresponding closest Tsukamurella species respectively.
31738158	7	23	theme	in	1057:1058	arg1	comparison					1084:1093	in silico genome-to-genome comparison	1057:1093	in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values]	1057:1195	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	4	24	with	patients	335:342	arg1	bacteraemia					365:375	bacteraemia	365:375	bacteraemia	365:375	Three bacterial strains, HKU70T, HKU71T and HKU72T, were isolated from the conjunctival swab, blood and sputum samples of three patients with conjunctivitis, bacteraemia and respiratory infection, respectively, in Hong Kong.
31738158	4	24	with	patients	335:342	arg1	infection					393:401	respiratory infection	381:401	respiratory infection	381:401	Three bacterial strains, HKU70T, HKU71T and HKU72T, were isolated from the conjunctival swab, blood and sputum samples of three patients with conjunctivitis, bacteraemia and respiratory infection, respectively, in Hong Kong.
31738158	4	24	with	patients	335:342	arg1	conjunctivitis					349:362	conjunctivitis	349:362	conjunctivitis	349:362	Three bacterial strains, HKU70T, HKU71T and HKU72T, were isolated from the conjunctival swab, blood and sputum samples of three patients with conjunctivitis, bacteraemia and respiratory infection, respectively, in Hong Kong.
31738158	7	25	theme	DNA-DNA	1011:1017	arg1	Tsukamurella					974:985	the genus Tsukamurella	964:985	the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness)	964:1030	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	7	25	theme	DNA-DNA	1011:1017	arg1	relatedness					1019:1029	26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness	988:1029	26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness	988:1029	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	5	26	from	species	640:646	arg1	distinguishable					585:599	distinguishable	585:599	distinguishable	585:599	The three strains were aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from currently recognized Tsukamurella species.
31738158	4	27	theme	bacterial	213:221	arg1	strains					223:229	Three bacterial strains	207:229	Three bacterial strains	207:229	Three bacterial strains, HKU70T, HKU71T and HKU72T, were isolated from the conjunctival swab, blood and sputum samples of three patients with conjunctivitis, bacteraemia and respiratory infection, respectively, in Hong Kong.
31738158	4	27	theme	bacterial	213:221	arg1	HKU72T					251:256	HKU72T	251:256	HKU72T	251:256	Three bacterial strains, HKU70T, HKU71T and HKU72T, were isolated from the conjunctival swab, blood and sputum samples of three patients with conjunctivitis, bacteraemia and respiratory infection, respectively, in Hong Kong.
31738158	4	27	theme	bacterial	213:221	arg1	HKU71T					240:245	HKU71T	240:245	HKU71T	240:245	Three bacterial strains, HKU70T, HKU71T and HKU72T, were isolated from the conjunctival swab, blood and sputum samples of three patients with conjunctivitis, bacteraemia and respiratory infection, respectively, in Hong Kong.
31738158	7	28	dep	in	1057:1058	arg1	silico					1060:1065	silico	1060:1065	silico	1060:1065	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	9	29	dep	strains	1387:1393	arg1	HKU72T					1414:1419	HKU72T	1414:1419	HKU72T	1414:1419	The G+C content determined based on the genome sequence of strains HKU70T, HKU71T and HKU72T were 69.9, 70.2 and 70.5 mol%, respectively.
31738158	9	29	dep	strains	1387:1393	arg1	strains					1387:1393	strains HKU70T, HKU71T and HKU72T	1387:1419	strains HKU70T, HKU71T and HKU72T	1387:1419	The G+C content determined based on the genome sequence of strains HKU70T, HKU71T and HKU72T were 69.9, 70.2 and 70.5 mol%, respectively.
31738158	9	29	dep	strains	1387:1393	arg1	HKU71T					1403:1408	HKU71T	1403:1408	HKU71T	1403:1408	The G+C content determined based on the genome sequence of strains HKU70T, HKU71T and HKU72T were 69.9, 70.2 and 70.5 mol%, respectively.
31738158	9	29	dep	strains	1387:1393	arg1	HKU70T					1395:1400	HKU70T	1395:1400	HKU70T	1395:1400	The G+C content determined based on the genome sequence of strains HKU70T, HKU71T and HKU72T were 69.9, 70.2 and 70.5 mol%, respectively.
31738158	9	30	theme	G+C	1332:1334	arg1	%					1449:1449	69.9, 70.2 and 70.5 mol%	1426:1449	%	1449:1449	The G+C content determined based on the genome sequence of strains HKU70T, HKU71T and HKU72T were 69.9, 70.2 and 70.5 mol%, respectively.
31738158	9	30	theme	G+C	1332:1334	arg1	content					1336:1342	The G+C content	1328:1342	The G+C content determined based on the genome sequence of strains HKU70T, HKU71T and HKU72T	1328:1419	The G+C content determined based on the genome sequence of strains HKU70T, HKU71T and HKU72T were 69.9, 70.2 and 70.5 mol%, respectively.
31738158	3	31	from	infection	183:191	arg1	Kong					201:204	Kong	201:204	Kong	201:204	nov., isolated from patients with bacteraemia, conjunctivitis and respiratory infection in Hong Kong.
31738158	7	32	with	line	1036:1039	arg1	results					1046:1052	results	1046:1052	results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values]	1046:1195	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	2	33	dep	sp	101:102	arg1	nov.					73:76	nov.	73:76	nov.	73:76	nov. and Tsukamurella sputi sp.
31738158	2	33	dep	sp	101:102	arg1	Tsukamurella					82:93	Tsukamurella	82:93	Tsukamurella	82:93	nov. and Tsukamurella sputi sp.
31738158	12	34	dep	sp	1763:1764	arg1	nov.					1691:1694	nov.	1691:1694	nov.	1691:1694	nov. and Tsukamurella conjunctivitidis HKU72T (=JCM 33389T=DSM 109108T) sp.
31738158	12	34	dep	sp	1763:1764	arg1	109108T					1754:1760	=JCM 33389T=DSM 109108T	1738:1760	=JCM 33389T=DSM 109108T	1738:1760	nov. and Tsukamurella conjunctivitidis HKU72T (=JCM 33389T=DSM 109108T) sp.
31738158	12	34	dep	sp	1763:1764	arg1	HKU72T					1730:1735	Tsukamurella conjunctivitidis HKU72T	1700:1735	Tsukamurella conjunctivitidis HKU72T	1700:1735	nov. and Tsukamurella conjunctivitidis HKU72T (=JCM 33389T=DSM 109108T) sp.
31738158	5	35	theme	Tsukamurella	627:638	arg1	species					640:646	currently recognized Tsukamurella species	606:646	currently recognized Tsukamurella species	606:646	The three strains were aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from currently recognized Tsukamurella species.
31738158	7	36	from	distinct	927:934	arg1	line					1036:1039	line	1036:1039	line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values]	1036:1195	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	5	37	theme	biochemical	564:574	arg1	profiles					576:583	unique biochemical profiles	557:583	unique biochemical profiles distinguishable from currently recognized Tsukamurella species	557:646	The three strains were aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from currently recognized Tsukamurella species.
31738158	4	38	theme	swab	295:298	arg1	samples					318:324	the conjunctival swab, blood and sputum samples	278:324	samples	318:324	Three bacterial strains, HKU70T, HKU71T and HKU72T, were isolated from the conjunctival swab, blood and sputum samples of three patients with conjunctivitis, bacteraemia and respiratory infection, respectively, in Hong Kong.
31738158	0	39	theme	Tsukamurella	0:11	arg1	sp					29:30	Tsukamurella asaccharolytica sp	0:30	Tsukamurella asaccharolytica sp.	0:31	Tsukamurella asaccharolytica sp.
31738158	9	40	theme	genome	1368:1373	arg1	sequence					1375:1382	the genome sequence	1364:1382	the genome sequence of strains HKU70T, HKU71T and HKU72T	1364:1419	The G+C content determined based on the genome sequence of strains HKU70T, HKU71T and HKU72T were 69.9, 70.2 and 70.5 mol%, respectively.
31738158	5	41	theme	distinguishable	585:599	arg1	profiles					576:583	unique biochemical profiles	557:583	unique biochemical profiles distinguishable from currently recognized Tsukamurella species	557:646	The three strains were aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from currently recognized Tsukamurella species.
31738158	7	42	from	species	953:959	arg1	distinct					927:934	distinct	927:934	distinct	927:934	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	6	43	theme	sequence	685:692	arg1	analyses					694:701	gene sequence analyses	680:701	gene sequence analyses	680:701	16S rRNA, secA, rpoB and groEL gene sequence analyses revealed that the three strains shared 99.6-99.9, 94.5-96.8, 95.7-97.8 and 97.7-98.9 % nucleotide identities with their corresponding closest Tsukamurella species respectively.
31738158	11	44	dep	HKU71T	1654:1659	arg1	Tsukamurella					1625:1636	Tsukamurella	1625:1636	Tsukamurella	1625:1636	nov., Tsukamurella asaccharolytica HKU71T (=JCM 33388T=DSM 109107T) sp.
31738158	4	45	theme	patients	335:342	arg1	samples					318:324	the conjunctival swab, blood and sputum samples	278:324	samples	318:324	Three bacterial strains, HKU70T, HKU71T and HKU72T, were isolated from the conjunctival swab, blood and sputum samples of three patients with conjunctivitis, bacteraemia and respiratory infection, respectively, in Hong Kong.
31738158	11	46	dep	sp	1687:1688	arg1	HKU71T					1654:1659	HKU71T	1654:1659	HKU71T	1654:1659	nov., Tsukamurella asaccharolytica HKU71T (=JCM 33388T=DSM 109107T) sp.
31738158	11	46	dep	sp	1687:1688	arg1	109107T					1678:1684	=JCM 33388T=DSM 109107T	1662:1684	=JCM 33388T=DSM 109107T	1662:1684	nov., Tsukamurella asaccharolytica HKU71T (=JCM 33388T=DSM 109107T) sp.
31738158	4	47	theme	blood	301:305	arg1	samples					318:324	the conjunctival swab, blood and sputum samples	278:324	samples	318:324	Three bacterial strains, HKU70T, HKU71T and HKU72T, were isolated from the conjunctival swab, blood and sputum samples of three patients with conjunctivitis, bacteraemia and respiratory infection, respectively, in Hong Kong.
31738158	7	48	dep	comparison	1084:1093	arg1	Calculator					1134:1143	32.2-40.9 % Genome-to-Genome Distance Calculator	1096:1143	32.2-40.9 % Genome-to-Genome Distance Calculator	1096:1143	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	7	48	dep	comparison	1084:1093	arg1	identity					1180:1187	86.3-88.9 % average nucleotide identity	1149:1187	86.3-88.9 % average nucleotide identity	1149:1187	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	7	49	theme	known	947:951	arg1	species					953:959	other known species	941:959	other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness)	941:1030	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	9	50	theme	69.9	1426:1429	arg1	%					1449:1449	69.9, 70.2 and 70.5 mol%	1426:1449	%	1449:1449	The G+C content determined based on the genome sequence of strains HKU70T, HKU71T and HKU72T were 69.9, 70.2 and 70.5 mol%, respectively.
31738158	9	50	theme	69.9	1426:1429	arg1	content					1336:1342	The G+C content	1328:1342	The G+C content determined based on the genome sequence of strains HKU70T, HKU71T and HKU72T	1328:1419	The G+C content determined based on the genome sequence of strains HKU70T, HKU71T and HKU72T were 69.9, 70.2 and 70.5 mol%, respectively.
31738158	1	51	theme	conjunctivitidis	52:67	arg1	sp					69:70	Tsukamurella conjunctivitidis sp	39:70	Tsukamurella conjunctivitidis sp	39:70	nov., Tsukamurella conjunctivitidis sp.
31738158	1	51	theme	conjunctivitidis	52:67	arg1	nov.					33:36	nov.	33:36	nov.	33:36	nov., Tsukamurella conjunctivitidis sp.
31738158	4	52	theme	sputum	311:316	arg1	samples					318:324	the conjunctival swab, blood and sputum samples	278:324	samples	318:324	Three bacterial strains, HKU70T, HKU71T and HKU72T, were isolated from the conjunctival swab, blood and sputum samples of three patients with conjunctivitis, bacteraemia and respiratory infection, respectively, in Hong Kong.
31738158	10	53	theme	species	1549:1555	arg1	description					1524:1534	description	1524:1534	description	1524:1534	Taken together, our results supported the proposition and description of three new species, i.e. Tsukamurella sputi HKU70T (=JCM 33387T=DSM 109106T) sp.
31738158	10	53	theme	species	1549:1555	arg1	proposition					1508:1518	proposition	1508:1518	proposition	1508:1518	Taken together, our results supported the proposition and description of three new species, i.e. Tsukamurella sputi HKU70T (=JCM 33387T=DSM 109106T) sp.
31738158	7	54	theme	average	1161:1167	arg1	identity					1180:1187	86.3-88.9 % average nucleotide identity	1149:1187	86.3-88.9 % average nucleotide identity	1149:1187	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	4	55	theme	respiratory	381:391	arg1	infection					393:401	respiratory infection	381:401	respiratory infection	381:401	Three bacterial strains, HKU70T, HKU71T and HKU72T, were isolated from the conjunctival swab, blood and sputum samples of three patients with conjunctivitis, bacteraemia and respiratory infection, respectively, in Hong Kong.
31738158	7	56	theme	86.3-88.9	1149:1157	arg1	%					1159:1159	%	1159:1159	%	1159:1159	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	6	57	theme	corresponding	823:835	arg1	species					858:864	their corresponding closest Tsukamurella species	817:864	their corresponding closest Tsukamurella species	817:864	16S rRNA, secA, rpoB and groEL gene sequence analyses revealed that the three strains shared 99.6-99.9, 94.5-96.8, 95.7-97.8 and 97.7-98.9 % nucleotide identities with their corresponding closest Tsukamurella species respectively.
31738158	9	58	theme	70.5	1441:1444	arg1	%					1449:1449	69.9, 70.2 and 70.5 mol%	1426:1449	%	1449:1449	The G+C content determined based on the genome sequence of strains HKU70T, HKU71T and HKU72T were 69.9, 70.2 and 70.5 mol%, respectively.
31738158	9	58	theme	70.5	1441:1444	arg1	content					1336:1342	The G+C content	1328:1342	The G+C content determined based on the genome sequence of strains HKU70T, HKU71T and HKU72T	1328:1419	The G+C content determined based on the genome sequence of strains HKU70T, HKU71T and HKU72T were 69.9, 70.2 and 70.5 mol%, respectively.
31738158	8	59	theme	Fatty	1198:1202	arg1	acids					1204:1208	Fatty acids	1198:1208	Fatty acids	1198:1208	Fatty acids, mycolic acids, cell-wall sugars and peptidoglycan analyses showed that they were typical of members of Tsukamurella.
31738158	6	60	dep	%	788:788	arg1	97.7-98.9 					778:787	97.7-98.9 	778:787	97.7-98.9 	778:787	16S rRNA, secA, rpoB and groEL gene sequence analyses revealed that the three strains shared 99.6-99.9, 94.5-96.8, 95.7-97.8 and 97.7-98.9 % nucleotide identities with their corresponding closest Tsukamurella species respectively.
31738158	6	60	dep	%	788:788	arg1	95.7-97.8					764:772	95.7-97.8	764:772	95.7-97.8	764:772	16S rRNA, secA, rpoB and groEL gene sequence analyses revealed that the three strains shared 99.6-99.9, 94.5-96.8, 95.7-97.8 and 97.7-98.9 % nucleotide identities with their corresponding closest Tsukamurella species respectively.
31738158	10	61	theme	sputi	1576:1580	arg1	109106T					1606:1612	=JCM 33387T=DSM 109106T	1590:1612	=JCM 33387T=DSM 109106T	1590:1612	Taken together, our results supported the proposition and description of three new species, i.e. Tsukamurella sputi HKU70T (=JCM 33387T=DSM 109106T) sp.
31738158	10	61	theme	sputi	1576:1580	arg1	HKU70T					1582:1587	Tsukamurella sputi HKU70T	1563:1587	Tsukamurella sputi HKU70T (=JCM 33387T=DSM 109106T) sp	1563:1616	Taken together, our results supported the proposition and description of three new species, i.e. Tsukamurella sputi HKU70T (=JCM 33387T=DSM 109106T) sp.
31738158	5	62	theme	positive	475:482	arg1	strains					442:448	The three strains	432:448	The three strains	432:448	The three strains were aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from currently recognized Tsukamurella species.
31738158	5	62	theme	positive	475:482	arg1	bacilli					535:541	aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli	455:541	aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli	455:541	The three strains were aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from currently recognized Tsukamurella species.
31738158	12	63	theme	33389T=DSM	1743:1752	arg1	109108T					1754:1760	=JCM 33389T=DSM 109108T	1738:1760	=JCM 33389T=DSM 109108T	1738:1760	nov. and Tsukamurella conjunctivitidis HKU72T (=JCM 33389T=DSM 109108T) sp.
31738158	12	63	theme	33389T=DSM	1743:1752	arg1	nov.					1691:1694	nov.	1691:1694	nov.	1691:1694	nov. and Tsukamurella conjunctivitidis HKU72T (=JCM 33389T=DSM 109108T) sp.
31738158	6	64	dep	identities	801:810	arg1	%					788:788	%	788:788	%	788:788	16S rRNA, secA, rpoB and groEL gene sequence analyses revealed that the three strains shared 99.6-99.9, 94.5-96.8, 95.7-97.8 and 97.7-98.9 % nucleotide identities with their corresponding closest Tsukamurella species respectively.
31738158	11	65	theme	33388T=DSM	1667:1676	arg1	HKU71T					1654:1659	HKU71T	1654:1659	HKU71T	1654:1659	nov., Tsukamurella asaccharolytica HKU71T (=JCM 33388T=DSM 109107T) sp.
31738158	11	65	theme	33388T=DSM	1667:1676	arg1	109107T					1678:1684	=JCM 33388T=DSM 109107T	1662:1684	=JCM 33388T=DSM 109107T	1662:1684	nov., Tsukamurella asaccharolytica HKU71T (=JCM 33388T=DSM 109107T) sp.
31738158	7	66	theme	Genome-to-Genome	1108:1123	arg1	Calculator					1134:1143	32.2-40.9 % Genome-to-Genome Distance Calculator	1096:1143	32.2-40.9 % Genome-to-Genome Distance Calculator	1096:1143	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	6	67	theme	nucleotide	790:799	arg1	identities					801:810	99.6-99.9, 94.5-96.8, 95.7-97.8 and 97.7-98.9 % nucleotide identities	742:810	99.6-99.9, 94.5-96.8, 95.7-97.8 and 97.7-98.9 % nucleotide identities	742:810	16S rRNA, secA, rpoB and groEL gene sequence analyses revealed that the three strains shared 99.6-99.9, 94.5-96.8, 95.7-97.8 and 97.7-98.9 % nucleotide identities with their corresponding closest Tsukamurella species respectively.
31738158	5	68	theme	catalase-positive	485:501	arg1	strains					442:448	The three strains	432:448	The three strains	432:448	The three strains were aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from currently recognized Tsukamurella species.
31738158	5	68	theme	catalase-positive	485:501	arg1	bacilli					535:541	aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli	455:541	aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli	455:541	The three strains were aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from currently recognized Tsukamurella species.
31738158	3	69	from	bacteraemia	139:149	arg1	Kong					201:204	Kong	201:204	Kong	201:204	nov., isolated from patients with bacteraemia, conjunctivitis and respiratory infection in Hong Kong.
31738158	10	70	theme	33387T=DSM	1595:1604	arg1	109106T					1606:1612	=JCM 33387T=DSM 109106T	1590:1612	=JCM 33387T=DSM 109106T	1590:1612	Taken together, our results supported the proposition and description of three new species, i.e. Tsukamurella sputi HKU70T (=JCM 33387T=DSM 109106T) sp.
31738158	10	70	theme	33387T=DSM	1595:1604	arg1	HKU70T					1582:1587	Tsukamurella sputi HKU70T	1563:1587	Tsukamurella sputi HKU70T (=JCM 33387T=DSM 109106T) sp	1563:1616	Taken together, our results supported the proposition and description of three new species, i.e. Tsukamurella sputi HKU70T (=JCM 33387T=DSM 109106T) sp.
31738158	7	71	theme	32.2-40.9 	1096:1105	arg1	%					1106:1106	%	1106:1106	%	1106:1106	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	8	72	theme	cell-wall	1226:1234	arg1	sugars					1236:1241	cell-wall sugars	1226:1241	cell-wall sugars	1226:1241	Fatty acids, mycolic acids, cell-wall sugars and peptidoglycan analyses showed that they were typical of members of Tsukamurella.
31738158	7	73	from	line	1036:1039	arg1	distinct					927:934	distinct	927:934	distinct	927:934	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	7	74	theme	comparison	1084:1093	arg1	results					1046:1052	results	1046:1052	results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values]	1046:1195	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	7	75	dep	Calculator	1134:1143	arg1	values					1189:1194	values	1189:1194	values	1189:1194	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	5	76	theme	unique	557:562	arg1	profiles					576:583	unique biochemical profiles	557:583	unique biochemical profiles distinguishable from currently recognized Tsukamurella species	557:646	The three strains were aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from currently recognized Tsukamurella species.
31738158	5	77	theme	recognized	616:625	arg1	species					640:646	currently recognized Tsukamurella species	606:646	currently recognized Tsukamurella species	606:646	The three strains were aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from currently recognized Tsukamurella species.
31738158	12	78	theme	Tsukamurella	1700:1711	arg1	HKU72T					1730:1735	Tsukamurella conjunctivitidis HKU72T	1700:1735	Tsukamurella conjunctivitidis HKU72T	1700:1735	nov. and Tsukamurella conjunctivitidis HKU72T (=JCM 33389T=DSM 109108T) sp.
31738158	3	79	with	patients	125:132	arg1	conjunctivitis					152:165	conjunctivitis	152:165	conjunctivitis	152:165	nov., isolated from patients with bacteraemia, conjunctivitis and respiratory infection in Hong Kong.
31738158	3	79	with	patients	125:132	arg1	infection					183:191	respiratory infection	171:191	respiratory infection	171:191	nov., isolated from patients with bacteraemia, conjunctivitis and respiratory infection in Hong Kong.
31738158	3	79	with	patients	125:132	arg1	bacteraemia					139:149	bacteraemia	139:149	bacteraemia	139:149	nov., isolated from patients with bacteraemia, conjunctivitis and respiratory infection in Hong Kong.
31738158	4	80	attach	isolated	264:271	arg2	HKU72T					251:256	HKU72T	251:256	HKU72T	251:256	Three bacterial strains, HKU70T, HKU71T and HKU72T, were isolated from the conjunctival swab, blood and sputum samples of three patients with conjunctivitis, bacteraemia and respiratory infection, respectively, in Hong Kong.
31738158	4	80	attach	isolated	264:271	arg2	HKU71T					240:245	HKU71T	240:245	HKU71T	240:245	Three bacterial strains, HKU70T, HKU71T and HKU72T, were isolated from the conjunctival swab, blood and sputum samples of three patients with conjunctivitis, bacteraemia and respiratory infection, respectively, in Hong Kong.
31738158	4	80	attach	isolated	264:271	arg1	samples					318:324	the conjunctival swab, blood and sputum samples	278:324	samples	318:324	Three bacterial strains, HKU70T, HKU71T and HKU72T, were isolated from the conjunctival swab, blood and sputum samples of three patients with conjunctivitis, bacteraemia and respiratory infection, respectively, in Hong Kong.
31738158	4	80	attach	isolated	264:271	arg2	strains					223:229	Three bacterial strains	207:229	Three bacterial strains	207:229	Three bacterial strains, HKU70T, HKU71T and HKU72T, were isolated from the conjunctival swab, blood and sputum samples of three patients with conjunctivitis, bacteraemia and respiratory infection, respectively, in Hong Kong.
31738158	5	81	dep	aerobic	455:461	arg1	Gram-stain					464:473	Gram-stain	464:473	Gram-stain	464:473	The three strains were aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from currently recognized Tsukamurella species.
31738158	7	82	dep	36.8±1.2 	1000:1008	arg1	to					997:998	to	997:998	to	997:998	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	5	83	theme	non-sporulating	504:518	arg1	strains					442:448	The three strains	432:448	The three strains	432:448	The three strains were aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from currently recognized Tsukamurella species.
31738158	5	83	theme	non-sporulating	504:518	arg1	bacilli					535:541	aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli	455:541	aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli	455:541	The three strains were aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from currently recognized Tsukamurella species.
31738158	3	84	from	conjunctivitis	152:165	arg1	Kong					201:204	Kong	201:204	Kong	201:204	nov., isolated from patients with bacteraemia, conjunctivitis and respiratory infection in Hong Kong.
31738158	5	85	theme	non-motile	524:533	arg1	strains					442:448	The three strains	432:448	The three strains	432:448	The three strains were aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from currently recognized Tsukamurella species.
31738158	5	85	theme	non-motile	524:533	arg1	bacilli					535:541	aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli	455:541	aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli	455:541	The three strains were aerobic, Gram-stain positive, catalase-positive, non-sporulating and non-motile bacilli and exhibited unique biochemical profiles distinguishable from currently recognized Tsukamurella species.
31738158	3	86	theme	respiratory	171:181	arg1	infection					183:191	respiratory infection	171:191	respiratory infection	171:191	nov., isolated from patients with bacteraemia, conjunctivitis and respiratory infection in Hong Kong.
31738158	6	87	theme	16S	649:651	arg1	rRNA					653:656	16S rRNA	649:656	16S rRNA	649:656	16S rRNA, secA, rpoB and groEL gene sequence analyses revealed that the three strains shared 99.6-99.9, 94.5-96.8, 95.7-97.8 and 97.7-98.9 % nucleotide identities with their corresponding closest Tsukamurella species respectively.
31738158	7	88	theme	%	1009:1009	arg1	Tsukamurella					974:985	the genus Tsukamurella	964:985	the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness)	964:1030	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	7	88	theme	%	1009:1009	arg1	relatedness					1019:1029	26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness	988:1029	26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness	988:1029	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	10	89	dep	proposition	1508:1518	arg1	the					1504:1506	the	1504:1506	the	1504:1506	Taken together, our results supported the proposition and description of three new species, i.e. Tsukamurella sputi HKU70T (=JCM 33387T=DSM 109106T) sp.
31738158	0	90	theme	asaccharolytica	13:27	arg1	sp					29:30	Tsukamurella asaccharolytica sp	0:30	Tsukamurella asaccharolytica sp.	0:31	Tsukamurella asaccharolytica sp.
31738158	4	91	theme	conjunctival	282:293	arg1	samples					318:324	the conjunctival swab, blood and sputum samples	278:324	samples	318:324	Three bacterial strains, HKU70T, HKU71T and HKU72T, were isolated from the conjunctival swab, blood and sputum samples of three patients with conjunctivitis, bacteraemia and respiratory infection, respectively, in Hong Kong.
31738158	7	92	theme	genus	968:972	arg1	Tsukamurella					974:985	the genus Tsukamurella	964:985	the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness)	964:1030	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	7	92	theme	genus	968:972	arg1	relatedness					1019:1029	26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness	988:1029	26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness	988:1029	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	6	93	theme	gene	680:683	arg1	analyses					694:701	gene sequence analyses	680:701	gene sequence analyses	680:701	16S rRNA, secA, rpoB and groEL gene sequence analyses revealed that the three strains shared 99.6-99.9, 94.5-96.8, 95.7-97.8 and 97.7-98.9 % nucleotide identities with their corresponding closest Tsukamurella species respectively.
31738158	9	94	theme	strains	1387:1393	arg1	sequence					1375:1382	the genome sequence	1364:1382	the genome sequence of strains HKU70T, HKU71T and HKU72T	1364:1419	The G+C content determined based on the genome sequence of strains HKU70T, HKU71T and HKU72T were 69.9, 70.2 and 70.5 mol%, respectively.
31738158	7	95	theme	Tsukamurella	974:985	arg1	species					953:959	other known species	941:959	other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness)	941:1030	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	7	96	theme	other	941:945	arg1	species					953:959	other known species	941:959	other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness)	941:1030	DNA-DNA hybridization confirmed that they were distinct from other known species of the genus Tsukamurella (26.2±2.4 to 36.8±1.2 % DNA-DNA relatedness), in line with results of in silico genome-to-genome comparison (32.2-40.9 % Genome-to-Genome Distance Calculator and 86.3-88.9 % average nucleotide identity values].
31738158	6	97	theme	Tsukamurella	845:856	arg1	species					858:864	their corresponding closest Tsukamurella species	817:864	their corresponding closest Tsukamurella species	817:864	16S rRNA, secA, rpoB and groEL gene sequence analyses revealed that the three strains shared 99.6-99.9, 94.5-96.8, 95.7-97.8 and 97.7-98.9 % nucleotide identities with their corresponding closest Tsukamurella species respectively.
31738158	1	98	theme	Tsukamurella	39:50	arg1	sp					69:70	Tsukamurella conjunctivitidis sp	39:70	Tsukamurella conjunctivitidis sp	39:70	nov., Tsukamurella conjunctivitidis sp.
31738158	1	98	theme	Tsukamurella	39:50	arg1	nov.					33:36	nov.	33:36	nov.	33:36	nov., Tsukamurella conjunctivitidis sp.
32886595	10	0	theme	chemotaxonomic	1152:1165	arg1	features					1167:1174	its phylogenetic, phenotypic and chemotaxonomic features	1119:1174	its phylogenetic, phenotypic and chemotaxonomic features	1119:1174	On the basis of its phylogenetic, phenotypic and chemotaxonomic features, strain E918T was considered to represent a novel species of the genus Arthrobacter, for which the name Arthrobacter mobilis sp.
32886595	6	1	theme	polar	684:688	arg1	profile					696:702	The polar lipid profile	680:702	The polar lipid profile	680:702	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two unidentified glycolipids.
32886595	3	2	theme	CGMCC	505:509	arg1	1.15091T					511:518	Arthrobacter deserti CGMCC 1.15091T	484:518	Arthrobacter deserti CGMCC 1.15091T (97.2 % similarity)	484:538	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain E918T belonged to the genus Arthrobacter and was most closely related to Arthrobacter deserti CGMCC 1.15091T (97.2 % similarity).
32886595	3	2	theme	CGMCC	505:509	arg1	similarity					528:537	97.2 % similarity	521:537	97.2 % similarity	521:537	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain E918T belonged to the genus Arthrobacter and was most closely related to Arthrobacter deserti CGMCC 1.15091T (97.2 % similarity).
32886595	9	3	theme	A.	1042:1043	arg1	1.15091T					1059:1066	A. deserti CGMCC 1.15091T	1042:1066	A. deserti CGMCC 1.15091T	1042:1066	The digital DNA-DNA hybridization and average nucleotide identity values between strain E918T and A. deserti CGMCC 1.15091T were 28.0 and 83.4%, respectively.
32886595	4	4	theme	A3α	570:572	arg1	type					574:577	the A3α type	566:577	the A3α type	566:577	The peptidoglycan was of the A3α type and the whole-cell sugar profile was found to contain galactose.
32886595	10	5	theme	features	1167:1174	arg1	basis					1110:1114	the basis	1106:1114	the basis of its phylogenetic, phenotypic and chemotaxonomic features	1106:1174	On the basis of its phylogenetic, phenotypic and chemotaxonomic features, strain E918T was considered to represent a novel species of the genus Arthrobacter, for which the name Arthrobacter mobilis sp.
32886595	2	6	theme	Gram-stain-positive	94:112	arg1	motile					183:188	A Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-mycelium-forming, motile	92:188	A Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-mycelium-forming, motile	92:188	A Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-mycelium-forming, motile, rod-shaped with one polar flagellum actinobacterium, designated E918T, was isolated from a desert soil collected in Cholistan desert, Pakistan.
32886595	9	7	theme	CGMCC	1053:1057	arg1	1.15091T					1059:1066	A. deserti CGMCC 1.15091T	1042:1066	A. deserti CGMCC 1.15091T	1042:1066	The digital DNA-DNA hybridization and average nucleotide identity values between strain E918T and A. deserti CGMCC 1.15091T were 28.0 and 83.4%, respectively.
32886595	10	8	theme	strain	1177:1182	arg1	E918T					1184:1188	strain E918T	1177:1188	strain E918T	1177:1188	On the basis of its phylogenetic, phenotypic and chemotaxonomic features, strain E918T was considered to represent a novel species of the genus Arthrobacter, for which the name Arthrobacter mobilis sp.
32886595	3	9	theme	16S	366:368	arg1	sequences					380:388	16S rRNA gene sequences	366:388	16S rRNA gene sequences	366:388	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain E918T belonged to the genus Arthrobacter and was most closely related to Arthrobacter deserti CGMCC 1.15091T (97.2 % similarity).
32886595	12	10	theme	33392T=CGMCC	1378:1389	arg1	1.16978T					1391:1398	=JCM 33392T=CGMCC 1.16978T	1373:1398	=JCM 33392T=CGMCC 1.16978T	1373:1398	The type strain of Arthrobacter mobilis is E918T (=JCM 33392T=CGMCC 1.16978T).
32886595	12	10	theme	33392T=CGMCC	1378:1389	arg1	E918T					1366:1370	E918T	1366:1370	E918T (=JCM 33392T=CGMCC 1.16978T)	1366:1399	The type strain of Arthrobacter mobilis is E918T (=JCM 33392T=CGMCC 1.16978T).
32886595	5	11	theme	major	648:652	arg1	MK-9					670:673	MK-9	670:673	MK-9	670:673	The major menaquinone was MK-9(H2).
32886595	5	11	theme	major	648:652	arg1	menaquinone					654:664	The major menaquinone	644:664	The major menaquinone	644:664	The major menaquinone was MK-9(H2).
32886595	2	12	theme	desert	282:287	arg1	soil					289:292	a desert soil	280:292	a desert soil collected in Cholistan desert, Pakistan	280:332	A Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-mycelium-forming, motile, rod-shaped with one polar flagellum actinobacterium, designated E918T, was isolated from a desert soil collected in Cholistan desert, Pakistan.
32886595	12	13	theme	=JCM	1373:1376	arg1	1.16978T					1391:1398	=JCM 33392T=CGMCC 1.16978T	1373:1398	=JCM 33392T=CGMCC 1.16978T	1373:1398	The type strain of Arthrobacter mobilis is E918T (=JCM 33392T=CGMCC 1.16978T).
32886595	12	13	theme	=JCM	1373:1376	arg1	E918T					1366:1370	E918T	1366:1370	E918T (=JCM 33392T=CGMCC 1.16978T)	1366:1399	The type strain of Arthrobacter mobilis is E918T (=JCM 33392T=CGMCC 1.16978T).
32886595	6	14	theme	unidentified	792:803	arg1	glycolipids					805:815	two unidentified glycolipids	788:815	two unidentified glycolipids	788:815	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two unidentified glycolipids.
32886595	3	15	theme	%	526:526	arg1	1.15091T					511:518	Arthrobacter deserti CGMCC 1.15091T	484:518	Arthrobacter deserti CGMCC 1.15091T (97.2 % similarity)	484:538	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain E918T belonged to the genus Arthrobacter and was most closely related to Arthrobacter deserti CGMCC 1.15091T (97.2 % similarity).
32886595	3	15	theme	%	526:526	arg1	similarity					528:537	97.2 % similarity	521:537	97.2 % similarity	521:537	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain E918T belonged to the genus Arthrobacter and was most closely related to Arthrobacter deserti CGMCC 1.15091T (97.2 % similarity).
32886595	3	16	theme	genus	433:437	arg1	Arthrobacter					439:450	the genus Arthrobacter	429:450	the genus Arthrobacter	429:450	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain E918T belonged to the genus Arthrobacter and was most closely related to Arthrobacter deserti CGMCC 1.15091T (97.2 % similarity).
32886595	9	17	theme	identity	1001:1008	arg1	%					1086:1086	28.0 and 83.4%	1073:1086	28.0 and 83.4%	1073:1086	The digital DNA-DNA hybridization and average nucleotide identity values between strain E918T and A. deserti CGMCC 1.15091T were 28.0 and 83.4%, respectively.
32886595	9	17	theme	identity	1001:1008	arg1	values					1010:1015	The digital DNA-DNA hybridization and average nucleotide identity values	944:1015	The digital DNA-DNA hybridization and average nucleotide identity values between strain E918T and A. deserti CGMCC 1.15091T	944:1066	The digital DNA-DNA hybridization and average nucleotide identity values between strain E918T and A. deserti CGMCC 1.15091T were 28.0 and 83.4%, respectively.
32886595	1	18	theme	Cholistan	69:77	arg1	soil					86:89	Cholistan desert soil	69:89	Cholistan desert soil	69:89	nov., a novel actinobacterium isolated from Cholistan desert soil.
32886595	10	19	theme	Arthrobacter	1247:1258	arg1	species					1226:1232	a novel species	1218:1232	a novel species	1218:1232	On the basis of its phylogenetic, phenotypic and chemotaxonomic features, strain E918T was considered to represent a novel species of the genus Arthrobacter, for which the name Arthrobacter mobilis sp.
32886595	9	20	theme	hybridization	964:976	arg1	%					1086:1086	28.0 and 83.4%	1073:1086	28.0 and 83.4%	1073:1086	The digital DNA-DNA hybridization and average nucleotide identity values between strain E918T and A. deserti CGMCC 1.15091T were 28.0 and 83.4%, respectively.
32886595	9	20	theme	hybridization	964:976	arg1	values					1010:1015	The digital DNA-DNA hybridization and average nucleotide identity values	944:1015	The digital DNA-DNA hybridization and average nucleotide identity values between strain E918T and A. deserti CGMCC 1.15091T	944:1066	The digital DNA-DNA hybridization and average nucleotide identity values between strain E918T and A. deserti CGMCC 1.15091T were 28.0 and 83.4%, respectively.
32886595	0	21	theme	mobilis	13:19	arg1	sp					21:22	Arthrobacter mobilis sp	0:22	Arthrobacter mobilis sp.	0:23	Arthrobacter mobilis sp.
32886595	1	22	theme	desert	79:84	arg1	soil					86:89	Cholistan desert soil	69:89	Cholistan desert soil	69:89	nov., a novel actinobacterium isolated from Cholistan desert soil.
32886595	10	23	theme	genus	1241:1245	arg1	Arthrobacter					1247:1258	the genus Arthrobacter	1237:1258	the genus Arthrobacter	1237:1258	On the basis of its phylogenetic, phenotypic and chemotaxonomic features, strain E918T was considered to represent a novel species of the genus Arthrobacter, for which the name Arthrobacter mobilis sp.
32886595	0	24	theme	Arthrobacter	0:11	arg1	sp					21:22	Arthrobacter mobilis sp	0:22	Arthrobacter mobilis sp.	0:23	Arthrobacter mobilis sp.
32886595	1	25	attach	isolated	55:62	arg2	actinobacterium					39:53	a novel actinobacterium	31:53	a novel actinobacterium isolated from Cholistan desert soil	31:89	nov., a novel actinobacterium isolated from Cholistan desert soil.
32886595	1	25	attach	isolated	55:62	arg1	soil					86:89	Cholistan desert soil	69:89	Cholistan desert soil	69:89	nov., a novel actinobacterium isolated from Cholistan desert soil.
32886595	12	26	theme	type	1327:1330	arg1	E918T					1366:1370	E918T	1366:1370	E918T (=JCM 33392T=CGMCC 1.16978T)	1366:1399	The type strain of Arthrobacter mobilis is E918T (=JCM 33392T=CGMCC 1.16978T).
32886595	12	26	theme	type	1327:1330	arg1	strain					1332:1337	The type strain	1323:1337	The type strain of Arthrobacter mobilis	1323:1361	The type strain of Arthrobacter mobilis is E918T (=JCM 33392T=CGMCC 1.16978T).
32886595	10	27	theme	Arthrobacter	1280:1291	arg1	sp					1301:1302	the name Arthrobacter mobilis sp	1271:1302	the name Arthrobacter mobilis sp	1271:1302	On the basis of its phylogenetic, phenotypic and chemotaxonomic features, strain E918T was considered to represent a novel species of the genus Arthrobacter, for which the name Arthrobacter mobilis sp.
32886595	3	28	theme	rRNA	370:373	arg1	sequences					380:388	16S rRNA gene sequences	366:388	16S rRNA gene sequences	366:388	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain E918T belonged to the genus Arthrobacter and was most closely related to Arthrobacter deserti CGMCC 1.15091T (97.2 % similarity).
32886595	10	29	theme	phylogenetic	1123:1134	arg1	features					1167:1174	its phylogenetic, phenotypic and chemotaxonomic features	1119:1174	its phylogenetic, phenotypic and chemotaxonomic features	1119:1174	On the basis of its phylogenetic, phenotypic and chemotaxonomic features, strain E918T was considered to represent a novel species of the genus Arthrobacter, for which the name Arthrobacter mobilis sp.
32886595	9	30	dep	A.	1042:1043	arg1	deserti					1045:1051	deserti	1045:1051	deserti	1045:1051	The digital DNA-DNA hybridization and average nucleotide identity values between strain E918T and A. deserti CGMCC 1.15091T were 28.0 and 83.4%, respectively.
32886595	12	31	theme	Arthrobacter	1342:1353	arg1	mobilis					1355:1361	Arthrobacter mobilis	1342:1361	Arthrobacter mobilis	1342:1361	The type strain of Arthrobacter mobilis is E918T (=JCM 33392T=CGMCC 1.16978T).
32886595	10	32	theme	name	1275:1278	arg1	sp					1301:1302	the name Arthrobacter mobilis sp	1271:1302	the name Arthrobacter mobilis sp	1271:1302	On the basis of its phylogenetic, phenotypic and chemotaxonomic features, strain E918T was considered to represent a novel species of the genus Arthrobacter, for which the name Arthrobacter mobilis sp.
32886595	3	33	theme	gene	375:378	arg1	sequences					380:388	16S rRNA gene sequences	366:388	16S rRNA gene sequences	366:388	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain E918T belonged to the genus Arthrobacter and was most closely related to Arthrobacter deserti CGMCC 1.15091T (97.2 % similarity).
32886595	8	34	theme	G+C	897:899	arg1	%					941:941	68.69 mol%	932:941	68.69 mol%	932:941	The G+C content of the genomic DNA was 68.69 mol%.
32886595	8	34	theme	G+C	897:899	arg1	content					901:907	The G+C content	893:907	The G+C content of the genomic DNA	893:926	The G+C content of the genomic DNA was 68.69 mol%.
32886595	7	35	theme	major	822:826	arg1	acids					834:838	The major fatty acids	818:838	The major fatty acids identified	818:849	The major fatty acids identified were anteiso-C15 : 0 and anteiso-C17 : 0.
32886595	7	35	theme	major	822:826	arg1	anteiso-C15 					856:867	anteiso-C15 	856:867	anteiso-C15 	856:867	The major fatty acids identified were anteiso-C15 : 0 and anteiso-C17 : 0.
32886595	4	36	contain	contain	625:631	arg2	galactose					633:641	galactose	633:641	galactose	633:641	The peptidoglycan was of the A3α type and the whole-cell sugar profile was found to contain galactose.
32886595	4	36	contain	contain	625:631	arg1	profile					604:610	the whole-cell sugar profile	583:610	the whole-cell sugar profile	583:610	The peptidoglycan was of the A3α type and the whole-cell sugar profile was found to contain galactose.
32886595	7	37	theme	fatty	828:832	arg1	acids					834:838	The major fatty acids	818:838	The major fatty acids identified	818:849	The major fatty acids identified were anteiso-C15 : 0 and anteiso-C17 : 0.
32886595	7	37	theme	fatty	828:832	arg1	anteiso-C15 					856:867	anteiso-C15 	856:867	anteiso-C15 	856:867	The major fatty acids identified were anteiso-C15 : 0 and anteiso-C17 : 0.
32886595	12	38	theme	mobilis	1355:1361	arg1	E918T					1366:1370	E918T	1366:1370	E918T (=JCM 33392T=CGMCC 1.16978T)	1366:1399	The type strain of Arthrobacter mobilis is E918T (=JCM 33392T=CGMCC 1.16978T).
32886595	12	38	theme	mobilis	1355:1361	arg1	strain					1332:1337	The type strain	1323:1337	The type strain of Arthrobacter mobilis	1323:1361	The type strain of Arthrobacter mobilis is E918T (=JCM 33392T=CGMCC 1.16978T).
32886595	9	39	theme	average	982:988	arg1	identity					1001:1008	average nucleotide identity	982:1008	average nucleotide identity	982:1008	The digital DNA-DNA hybridization and average nucleotide identity values between strain E918T and A. deserti CGMCC 1.15091T were 28.0 and 83.4%, respectively.
32886595	9	40	theme	DNA-DNA	956:962	arg1	hybridization					964:976	digital DNA-DNA hybridization	948:976	digital DNA-DNA hybridization	948:976	The digital DNA-DNA hybridization and average nucleotide identity values between strain E918T and A. deserti CGMCC 1.15091T were 28.0 and 83.4%, respectively.
32886595	9	41	theme	nucleotide	990:999	arg1	identity					1001:1008	average nucleotide identity	982:1008	average nucleotide identity	982:1008	The digital DNA-DNA hybridization and average nucleotide identity values between strain E918T and A. deserti CGMCC 1.15091T were 28.0 and 83.4%, respectively.
32886595	2	42	dep	Gram-stain-positive	94:112	arg1	aerobic					115:121	aerobic	115:121	aerobic	115:121	A Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-mycelium-forming, motile, rod-shaped with one polar flagellum actinobacterium, designated E918T, was isolated from a desert soil collected in Cholistan desert, Pakistan.
32886595	2	42	dep	Gram-stain-positive	94:112	arg1	non-mycelium-forming					161:180	non-mycelium-forming	161:180	non-mycelium-forming	161:180	A Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-mycelium-forming, motile, rod-shaped with one polar flagellum actinobacterium, designated E918T, was isolated from a desert soil collected in Cholistan desert, Pakistan.
32886595	2	42	dep	Gram-stain-positive	94:112	arg1	oxidase-negative					143:158	oxidase-negative	143:158	oxidase-negative	143:158	A Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-mycelium-forming, motile, rod-shaped with one polar flagellum actinobacterium, designated E918T, was isolated from a desert soil collected in Cholistan desert, Pakistan.
32886595	2	42	dep	Gram-stain-positive	94:112	arg1	catalase-positive					124:140	catalase-positive	124:140	catalase-positive	124:140	A Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-mycelium-forming, motile, rod-shaped with one polar flagellum actinobacterium, designated E918T, was isolated from a desert soil collected in Cholistan desert, Pakistan.
32886595	10	43	theme	phenotypic	1137:1146	arg1	features					1167:1174	its phylogenetic, phenotypic and chemotaxonomic features	1119:1174	its phylogenetic, phenotypic and chemotaxonomic features	1119:1174	On the basis of its phylogenetic, phenotypic and chemotaxonomic features, strain E918T was considered to represent a novel species of the genus Arthrobacter, for which the name Arthrobacter mobilis sp.
32886595	7	44	dep	anteiso-C15 	856:867	arg1	 0					889:890	 0	889:890	anteiso-C15 : 0 and anteiso-C17 : 0	856:890	The major fatty acids identified were anteiso-C15 : 0 and anteiso-C17 : 0.
32886595	7	44	dep	anteiso-C15 	856:867	arg1	anteiso-C17 					876:887	anteiso-C17 	876:887	anteiso-C17 	876:887	The major fatty acids identified were anteiso-C15 : 0 and anteiso-C17 : 0.
32886595	7	44	dep	anteiso-C15 	856:867	arg1	 0					869:870	 0	869:870	 0	869:870	The major fatty acids identified were anteiso-C15 : 0 and anteiso-C17 : 0.
32886595	3	45	theme	Arthrobacter	484:495	arg1	1.15091T					511:518	Arthrobacter deserti CGMCC 1.15091T	484:518	Arthrobacter deserti CGMCC 1.15091T (97.2 % similarity)	484:538	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain E918T belonged to the genus Arthrobacter and was most closely related to Arthrobacter deserti CGMCC 1.15091T (97.2 % similarity).
32886595	3	45	theme	Arthrobacter	484:495	arg1	similarity					528:537	97.2 % similarity	521:537	97.2 % similarity	521:537	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain E918T belonged to the genus Arthrobacter and was most closely related to Arthrobacter deserti CGMCC 1.15091T (97.2 % similarity).
32886595	3	46	theme	strain	404:409	arg1	E918T					411:415	strain E918T	404:415	strain E918T	404:415	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain E918T belonged to the genus Arthrobacter and was most closely related to Arthrobacter deserti CGMCC 1.15091T (97.2 % similarity).
32886595	2	47	theme	polar	211:215	arg1	actinobacterium					227:241	one polar flagellum actinobacterium	207:241	one polar flagellum actinobacterium	207:241	A Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-mycelium-forming, motile, rod-shaped with one polar flagellum actinobacterium, designated E918T, was isolated from a desert soil collected in Cholistan desert, Pakistan.
32886595	2	48	theme	flagellum	217:225	arg1	actinobacterium					227:241	one polar flagellum actinobacterium	207:241	one polar flagellum actinobacterium	207:241	A Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-mycelium-forming, motile, rod-shaped with one polar flagellum actinobacterium, designated E918T, was isolated from a desert soil collected in Cholistan desert, Pakistan.
32886595	8	49	theme	DNA	924:926	arg1	%					941:941	68.69 mol%	932:941	68.69 mol%	932:941	The G+C content of the genomic DNA was 68.69 mol%.
32886595	8	49	theme	DNA	924:926	arg1	content					901:907	The G+C content	893:907	The G+C content of the genomic DNA	893:926	The G+C content of the genomic DNA was 68.69 mol%.
32886595	3	50	theme	deserti	497:503	arg1	1.15091T					511:518	Arthrobacter deserti CGMCC 1.15091T	484:518	Arthrobacter deserti CGMCC 1.15091T (97.2 % similarity)	484:538	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain E918T belonged to the genus Arthrobacter and was most closely related to Arthrobacter deserti CGMCC 1.15091T (97.2 % similarity).
32886595	3	50	theme	deserti	497:503	arg1	similarity					528:537	97.2 % similarity	521:537	97.2 % similarity	521:537	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain E918T belonged to the genus Arthrobacter and was most closely related to Arthrobacter deserti CGMCC 1.15091T (97.2 % similarity).
32886595	8	51	theme	genomic	916:922	arg1	DNA					924:926	the genomic DNA	912:926	the genomic DNA	912:926	The G+C content of the genomic DNA was 68.69 mol%.
32886595	1	52	dep	actinobacterium	39:53	arg1	nov.					25:28	nov.	25:28	nov.	25:28	nov., a novel actinobacterium isolated from Cholistan desert soil.
32886595	4	53	theme	whole-cell	587:596	arg1	profile					604:610	the whole-cell sugar profile	583:610	the whole-cell sugar profile	583:610	The peptidoglycan was of the A3α type and the whole-cell sugar profile was found to contain galactose.
32886595	10	54	theme	mobilis	1293:1299	arg1	sp					1301:1302	the name Arthrobacter mobilis sp	1271:1302	the name Arthrobacter mobilis sp	1271:1302	On the basis of its phylogenetic, phenotypic and chemotaxonomic features, strain E918T was considered to represent a novel species of the genus Arthrobacter, for which the name Arthrobacter mobilis sp.
32886595	10	55	theme	novel	1220:1224	arg1	species					1226:1232	a novel species	1218:1232	a novel species	1218:1232	On the basis of its phylogenetic, phenotypic and chemotaxonomic features, strain E918T was considered to represent a novel species of the genus Arthrobacter, for which the name Arthrobacter mobilis sp.
32886595	8	56	theme	68.69 mol	932:940	arg1	%					941:941	68.69 mol%	932:941	68.69 mol%	932:941	The G+C content of the genomic DNA was 68.69 mol%.
32886595	8	56	theme	68.69 mol	932:940	arg1	content					901:907	The G+C content	893:907	The G+C content of the genomic DNA	893:926	The G+C content of the genomic DNA was 68.69 mol%.
32886595	2	57	with	rod-shaped	191:200	arg1	actinobacterium					227:241	one polar flagellum actinobacterium	207:241	one polar flagellum actinobacterium	207:241	A Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-mycelium-forming, motile, rod-shaped with one polar flagellum actinobacterium, designated E918T, was isolated from a desert soil collected in Cholistan desert, Pakistan.
32886595	9	58	theme	strain	1025:1030	arg1	E918T					1032:1036	strain E918T	1025:1036	strain E918T	1025:1036	The digital DNA-DNA hybridization and average nucleotide identity values between strain E918T and A. deserti CGMCC 1.15091T were 28.0 and 83.4%, respectively.
32886595	2	59	attach	isolated	266:273	arg1	soil					289:292	a desert soil	280:292	a desert soil collected in Cholistan desert, Pakistan	280:332	A Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-mycelium-forming, motile, rod-shaped with one polar flagellum actinobacterium, designated E918T, was isolated from a desert soil collected in Cholistan desert, Pakistan.
32886595	2	59	attach	isolated	266:273	arg2	motile					183:188	A Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-mycelium-forming, motile	92:188	A Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-mycelium-forming, motile	92:188	A Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-mycelium-forming, motile, rod-shaped with one polar flagellum actinobacterium, designated E918T, was isolated from a desert soil collected in Cholistan desert, Pakistan.
32886595	2	60	theme	rod-shaped	191:200	arg1	motile					183:188	A Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-mycelium-forming, motile	92:188	A Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-mycelium-forming, motile	92:188	A Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-mycelium-forming, motile, rod-shaped with one polar flagellum actinobacterium, designated E918T, was isolated from a desert soil collected in Cholistan desert, Pakistan.
32886595	9	61	theme	digital	948:954	arg1	hybridization					964:976	digital DNA-DNA hybridization	948:976	digital DNA-DNA hybridization	948:976	The digital DNA-DNA hybridization and average nucleotide identity values between strain E918T and A. deserti CGMCC 1.15091T were 28.0 and 83.4%, respectively.
32886595	3	62	theme	Phylogenetic	335:346	arg1	analysis					348:355	Phylogenetic analysis	335:355	Phylogenetic analysis based on 16S rRNA gene sequences	335:388	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain E918T belonged to the genus Arthrobacter and was most closely related to Arthrobacter deserti CGMCC 1.15091T (97.2 % similarity).
32886595	1	63	theme	novel	33:37	arg1	actinobacterium					39:53	a novel actinobacterium	31:53	a novel actinobacterium isolated from Cholistan desert soil	31:89	nov., a novel actinobacterium isolated from Cholistan desert soil.
32886595	6	64	theme	lipid	690:694	arg1	profile					696:702	The polar lipid profile	680:702	The polar lipid profile	680:702	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two unidentified glycolipids.
32886595	3	65	theme	97.2 	521:525	arg1	%					526:526	%	526:526	%	526:526	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain E918T belonged to the genus Arthrobacter and was most closely related to Arthrobacter deserti CGMCC 1.15091T (97.2 % similarity).
32886595	4	66	theme	sugar	598:602	arg1	profile					604:610	the whole-cell sugar profile	583:610	the whole-cell sugar profile	583:610	The peptidoglycan was of the A3α type and the whole-cell sugar profile was found to contain galactose.
33273482	4	0	theme	systemic	846:853	arg1	levels					864:869	lower systemic ceramide levels	840:869	lower systemic ceramide levels	840:869	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	3	1	theme	16S	696:698	arg1	sequencing					705:714	16S rRNA sequencing	696:714	16S rRNA sequencing of cecum content	696:731	Herein, we investigated if these beneficial effects were associated with alterations in plasma metabolites, using metabolomic and lipidomic analysis, and gut microbiota composition, using 16S rRNA sequencing of cecum content, comparing NLRP3 deficient and wild type (WT) mice on HFD and control diet.
33273482	1	2	theme	problem	266:272	arg1	worldwide					274:282	an increasing health problem worldwide	245:282	an increasing health problem worldwide	245:282	Obesity-related diseases (e.g. type 2 diabetes mellitus and cardiovascular disorders) represent an increasing health problem worldwide.
33273482	6	3	theme	metabolic	1592:1600	arg1	stress					1619:1624	metabolic and inflammatory stress	1592:1624	metabolic and inflammatory stress	1592:1624	Our findings support a role of NLRP3 inflammasome in the interface between metabolic and inflammatory stress, involving an altered gut microbiota composition.
33273482	4	4	theme	deficient	821:829	arg1	mice					831:834	Obese NLRP3 deficient mice	809:834	Obese NLRP3 deficient mice	809:834	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	3	5	theme	cecum	719:723	arg1	content					725:731	cecum content	719:731	cecum content	719:731	Herein, we investigated if these beneficial effects were associated with alterations in plasma metabolites, using metabolomic and lipidomic analysis, and gut microbiota composition, using 16S rRNA sequencing of cecum content, comparing NLRP3 deficient and wild type (WT) mice on HFD and control diet.
33273482	2	6	theme	NLRP3	285:289	arg1	activation					304:313	NLRP3 inflammasome activation	285:313	NLRP3 inflammasome activation	285:313	NLRP3 inflammasome activation may underlie obesity-induced inflammation and insulin resistance, and NLRP3 deficient mice exposed to high fat diet (HFD) appear to be protected from left ventricle (LV) concentric remodeling.
33273482	1	7	theme	type	180:183	arg1	mellitus					196:203	type 2 diabetes mellitus	180:203	type 2 diabetes mellitus	180:203	Obesity-related diseases (e.g. type 2 diabetes mellitus and cardiovascular disorders) represent an increasing health problem worldwide.
33273482	5	8	theme	systemic	1451:1458	arg1	inflammation					1460:1471	systemic inflammation	1451:1471	systemic inflammation	1451:1471	These changes were accompanied by an altered composition of gut microbiota associated with decreased systemic levels of tri-methylamine-N-oxide and lipopolysaccharide, potentially inducing attenuating systemic inflammation and beneficial effects on lipid metabolism.
33273482	1	9	theme	diabetes	187:194	arg1	mellitus					196:203	type 2 diabetes mellitus	180:203	type 2 diabetes mellitus	180:203	Obesity-related diseases (e.g. type 2 diabetes mellitus and cardiovascular disorders) represent an increasing health problem worldwide.
33273482	4	10	theme	downregulated	1092:1104	arg1	metabolism					1124:1133	downregulated myocardial energy metabolism	1092:1133	downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue	1092:1186	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	3	11	theme	NLRP3	744:748	arg1	deficient					750:758	NLRP3 deficient	744:758	NLRP3 deficient	744:758	Herein, we investigated if these beneficial effects were associated with alterations in plasma metabolites, using metabolomic and lipidomic analysis, and gut microbiota composition, using 16S rRNA sequencing of cecum content, comparing NLRP3 deficient and wild type (WT) mice on HFD and control diet.
33273482	6	12	theme	NLRP3	1548:1552	arg1	inflammasome					1554:1565	NLRP3 inflammasome	1548:1565	NLRP3 inflammasome	1548:1565	Our findings support a role of NLRP3 inflammasome in the interface between metabolic and inflammatory stress, involving an altered gut microbiota composition.
33273482	4	13	theme	hepatic	928:934	arg1	expression					936:945	altered hepatic expression	920:945	altered hepatic expression of fatty acids (FA) with lower mono-saturated FA	920:994	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	4	14	theme	energy	1117:1122	arg1	metabolism					1124:1133	downregulated myocardial energy metabolism	1092:1133	downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue	1092:1186	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	0	15	theme	fat	139:141	arg1	diet					143:146	high fat diet	134:146	high fat diet	134:146	NLRP3 inflammasome deficiency attenuates metabolic disturbances involving alterations in the gut microbial profile in mice exposed to high fat diet.
33273482	4	16	dep	metabolism	1124:1133	arg1	assessed					1138:1145	assessed	1138:1145	assessed by proteomic analyses of LV heart tissue	1138:1186	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	4	17	theme	tissue	1181:1186	arg1	analyses					1160:1167	proteomic analyses	1150:1167	proteomic analyses of LV heart tissue	1150:1186	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	3	18	theme	type	769:772	arg1	mice					779:782	NLRP3 deficient and wild type (WT) mice	744:782	NLRP3 deficient and wild type (WT) mice	744:782	Herein, we investigated if these beneficial effects were associated with alterations in plasma metabolites, using metabolomic and lipidomic analysis, and gut microbiota composition, using 16S rRNA sequencing of cecum content, comparing NLRP3 deficient and wild type (WT) mice on HFD and control diet.
33273482	3	19	theme	plasma	596:601	arg1	metabolites					603:613	plasma metabolites	596:613	plasma metabolites	596:613	Herein, we investigated if these beneficial effects were associated with alterations in plasma metabolites, using metabolomic and lipidomic analysis, and gut microbiota composition, using 16S rRNA sequencing of cecum content, comparing NLRP3 deficient and wild type (WT) mice on HFD and control diet.
33273482	3	20	theme	lipidomic	638:646	arg1	analysis					648:655	metabolomic and lipidomic analysis	622:655	metabolomic and lipidomic analysis	622:655	Herein, we investigated if these beneficial effects were associated with alterations in plasma metabolites, using metabolomic and lipidomic analysis, and gut microbiota composition, using 16S rRNA sequencing of cecum content, comparing NLRP3 deficient and wild type (WT) mice on HFD and control diet.
33273482	0	21	from	profile	107:113	arg1	mice					118:121	mice	118:121	mice exposed to high fat diet	118:146	NLRP3 inflammasome deficiency attenuates metabolic disturbances involving alterations in the gut microbial profile in mice exposed to high fat diet.
33273482	3	22	from	alterations	581:591	arg1	metabolites					603:613	plasma metabolites	596:613	plasma metabolites	596:613	Herein, we investigated if these beneficial effects were associated with alterations in plasma metabolites, using metabolomic and lipidomic analysis, and gut microbiota composition, using 16S rRNA sequencing of cecum content, comparing NLRP3 deficient and wild type (WT) mice on HFD and control diet.
33273482	3	22	from	alterations	581:591	arg1	composition					677:687	gut microbiota composition	662:687	gut microbiota composition	662:687	Herein, we investigated if these beneficial effects were associated with alterations in plasma metabolites, using metabolomic and lipidomic analysis, and gut microbiota composition, using 16S rRNA sequencing of cecum content, comparing NLRP3 deficient and wild type (WT) mice on HFD and control diet.
33273482	3	22	from	alterations	581:591	arg1	using					616:620	using	616:620	using metabolomic and lipidomic analysis	616:655	Herein, we investigated if these beneficial effects were associated with alterations in plasma metabolites, using metabolomic and lipidomic analysis, and gut microbiota composition, using 16S rRNA sequencing of cecum content, comparing NLRP3 deficient and wild type (WT) mice on HFD and control diet.
33273482	4	23	dep	resulting	884:892	arg1	counteracting					1046:1058	counteracting	1046:1058	counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids	1046:1222	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	3	24	theme	metabolomic	622:632	arg1	analysis					648:655	metabolomic and lipidomic analysis	622:655	metabolomic and lipidomic analysis	622:655	Herein, we investigated if these beneficial effects were associated with alterations in plasma metabolites, using metabolomic and lipidomic analysis, and gut microbiota composition, using 16S rRNA sequencing of cecum content, comparing NLRP3 deficient and wild type (WT) mice on HFD and control diet.
33273482	6	25	theme	inflammatory	1606:1617	arg1	stress					1619:1624	metabolic and inflammatory stress	1592:1624	metabolic and inflammatory stress	1592:1624	Our findings support a role of NLRP3 inflammasome in the interface between metabolic and inflammatory stress, involving an altered gut microbiota composition.
33273482	1	26	theme	Obesity-related	149:163	arg1	diseases					165:172	Obesity-related diseases	149:172	Obesity-related diseases (e.g. type 2 diabetes mellitus and cardiovascular disorders)	149:233	Obesity-related diseases (e.g. type 2 diabetes mellitus and cardiovascular disorders) represent an increasing health problem worldwide.
33273482	2	27	theme	concentric	485:494	arg1	remodeling					496:505	concentric remodeling	485:505	left ventricle (LV) concentric remodeling	465:505	NLRP3 inflammasome activation may underlie obesity-induced inflammation and insulin resistance, and NLRP3 deficient mice exposed to high fat diet (HFD) appear to be protected from left ventricle (LV) concentric remodeling.
33273482	4	28	theme	LV	1172:1173	arg1	tissue					1181:1186	LV heart tissue	1172:1186	LV heart tissue	1172:1186	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	0	29	theme	gut	93:95	arg1	profile					107:113	the gut microbial profile	89:113	the gut microbial profile in mice exposed to high fat diet	89:146	NLRP3 inflammasome deficiency attenuates metabolic disturbances involving alterations in the gut microbial profile in mice exposed to high fat diet.
33273482	3	30	theme	control	795:801	arg1	diet					803:806	control diet	795:806	control diet	795:806	Herein, we investigated if these beneficial effects were associated with alterations in plasma metabolites, using metabolomic and lipidomic analysis, and gut microbiota composition, using 16S rRNA sequencing of cecum content, comparing NLRP3 deficient and wild type (WT) mice on HFD and control diet.
33273482	4	31	theme	acids	956:960	arg1	expression					936:945	altered hepatic expression	920:945	altered hepatic expression of fatty acids (FA) with lower mono-saturated FA	920:994	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	4	31	theme	acids	956:960	arg1	inflammation					906:917	inflammation	906:917	inflammation	906:917	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	4	31	theme	acids	956:960	arg1	levels					1026:1031	higher polyunsaturated FA levels	1000:1031	higher polyunsaturated FA levels	1000:1031	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	5	32	theme	lipid	1499:1503	arg1	metabolism					1505:1514	lipid metabolism	1499:1514	lipid metabolism	1499:1514	These changes were accompanied by an altered composition of gut microbiota associated with decreased systemic levels of tri-methylamine-N-oxide and lipopolysaccharide, potentially inducing attenuating systemic inflammation and beneficial effects on lipid metabolism.
33273482	4	33	theme	polyunsaturated	1007:1021	arg1	levels					1026:1031	higher polyunsaturated FA levels	1000:1031	higher polyunsaturated FA levels	1000:1031	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	5	34	theme	decreased	1341:1349	arg1	levels					1360:1365	decreased systemic levels	1341:1365	decreased systemic levels of tri-methylamine-N-oxide and lipopolysaccharide	1341:1415	These changes were accompanied by an altered composition of gut microbiota associated with decreased systemic levels of tri-methylamine-N-oxide and lipopolysaccharide, potentially inducing attenuating systemic inflammation and beneficial effects on lipid metabolism.
33273482	2	35	theme	high	417:420	arg1	HFD					432:434	HFD	432:434	HFD	432:434	NLRP3 inflammasome activation may underlie obesity-induced inflammation and insulin resistance, and NLRP3 deficient mice exposed to high fat diet (HFD) appear to be protected from left ventricle (LV) concentric remodeling.
33273482	2	35	theme	high	417:420	arg1	diet					426:429	high fat diet	417:429	high fat diet (HFD)	417:435	NLRP3 inflammasome activation may underlie obesity-induced inflammation and insulin resistance, and NLRP3 deficient mice exposed to high fat diet (HFD) appear to be protected from left ventricle (LV) concentric remodeling.
33273482	0	36	theme	NLRP3	0:4	arg1	deficiency					19:28	NLRP3 inflammasome deficiency	0:28	NLRP3 inflammasome deficiency	0:28	NLRP3 inflammasome deficiency attenuates metabolic disturbances involving alterations in the gut microbial profile in mice exposed to high fat diet.
33273482	4	37	theme	bile	1213:1216	arg1	acids					1218:1222	bile acids	1213:1222	bile acids	1213:1222	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	4	38	theme	mono-saturated	978:991	arg1	FA					993:994	lower mono-saturated FA	972:994	lower mono-saturated FA	972:994	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	3	39	theme	gut	662:664	arg1	composition					677:687	gut microbiota composition	662:687	gut microbiota composition	662:687	Herein, we investigated if these beneficial effects were associated with alterations in plasma metabolites, using metabolomic and lipidomic analysis, and gut microbiota composition, using 16S rRNA sequencing of cecum content, comparing NLRP3 deficient and wild type (WT) mice on HFD and control diet.
33273482	5	40	theme	gut	1310:1312	arg1	microbiota					1314:1323	gut microbiota	1310:1323	gut microbiota	1310:1323	These changes were accompanied by an altered composition of gut microbiota associated with decreased systemic levels of tri-methylamine-N-oxide and lipopolysaccharide, potentially inducing attenuating systemic inflammation and beneficial effects on lipid metabolism.
33273482	0	41	theme	metabolic	41:49	arg1	disturbances					51:62	metabolic disturbances	41:62	metabolic disturbances involving alterations in the gut microbial profile in mice exposed to high fat diet	41:146	NLRP3 inflammasome deficiency attenuates metabolic disturbances involving alterations in the gut microbial profile in mice exposed to high fat diet.
33273482	4	42	theme	Obese	809:813	arg1	mice					831:834	Obese NLRP3 deficient mice	809:834	Obese NLRP3 deficient mice	809:834	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	5	43	theme	lipopolysaccharide	1398:1415	arg1	levels					1360:1365	decreased systemic levels	1341:1365	decreased systemic levels of tri-methylamine-N-oxide and lipopolysaccharide	1341:1415	These changes were accompanied by an altered composition of gut microbiota associated with decreased systemic levels of tri-methylamine-N-oxide and lipopolysaccharide, potentially inducing attenuating systemic inflammation and beneficial effects on lipid metabolism.
33273482	5	44	from	inflammation	1460:1471	arg1	metabolism					1505:1514	lipid metabolism	1499:1514	lipid metabolism	1499:1514	These changes were accompanied by an altered composition of gut microbiota associated with decreased systemic levels of tri-methylamine-N-oxide and lipopolysaccharide, potentially inducing attenuating systemic inflammation and beneficial effects on lipid metabolism.
33273482	4	45	theme	liver	1075:1079	arg1	steatosis					1081:1089	liver steatosis	1075:1089	liver steatosis	1075:1089	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	4	46	with	acids	956:960	arg1	FA					993:994	lower mono-saturated FA	972:994	lower mono-saturated FA	972:994	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	1	47	dep	mellitus	196:203	arg1	e.g.					175:178	e.g.	175:178	e.g.	175:178	Obesity-related diseases (e.g. type 2 diabetes mellitus and cardiovascular disorders) represent an increasing health problem worldwide.
33273482	5	48	theme	tri-methylamine-N-oxide	1370:1392	arg1	levels					1360:1365	decreased systemic levels	1341:1365	decreased systemic levels of tri-methylamine-N-oxide and lipopolysaccharide	1341:1415	These changes were accompanied by an altered composition of gut microbiota associated with decreased systemic levels of tri-methylamine-N-oxide and lipopolysaccharide, potentially inducing attenuating systemic inflammation and beneficial effects on lipid metabolism.
33273482	2	49	theme	deficient	391:399	arg1	mice					401:404	NLRP3 deficient mice	385:404	NLRP3 deficient mice exposed to high fat diet (HFD)	385:435	NLRP3 inflammasome activation may underlie obesity-induced inflammation and insulin resistance, and NLRP3 deficient mice exposed to high fat diet (HFD) appear to be protected from left ventricle (LV) concentric remodeling.
33273482	1	50	theme	health	259:264	arg1	worldwide					274:282	an increasing health problem worldwide	245:282	an increasing health problem worldwide	245:282	Obesity-related diseases (e.g. type 2 diabetes mellitus and cardiovascular disorders) represent an increasing health problem worldwide.
33273482	4	51	theme	lower	840:844	arg1	levels					864:869	lower systemic ceramide levels	840:869	lower systemic ceramide levels	840:869	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	3	52	theme	rRNA	700:703	arg1	sequencing					705:714	16S rRNA sequencing	696:714	16S rRNA sequencing of cecum content	696:731	Herein, we investigated if these beneficial effects were associated with alterations in plasma metabolites, using metabolomic and lipidomic analysis, and gut microbiota composition, using 16S rRNA sequencing of cecum content, comparing NLRP3 deficient and wild type (WT) mice on HFD and control diet.
33273482	5	53	theme	beneficial	1477:1486	arg1	effects					1488:1494	beneficial effects	1477:1494	beneficial effects on lipid metabolism	1477:1514	These changes were accompanied by an altered composition of gut microbiota associated with decreased systemic levels of tri-methylamine-N-oxide and lipopolysaccharide, potentially inducing attenuating systemic inflammation and beneficial effects on lipid metabolism.
33273482	3	54	theme	beneficial	541:550	arg1	effects					552:558	these beneficial effects	535:558	these beneficial effects	535:558	Herein, we investigated if these beneficial effects were associated with alterations in plasma metabolites, using metabolomic and lipidomic analysis, and gut microbiota composition, using 16S rRNA sequencing of cecum content, comparing NLRP3 deficient and wild type (WT) mice on HFD and control diet.
33273482	4	55	theme	ceramide	855:862	arg1	levels					864:869	lower systemic ceramide levels	840:869	lower systemic ceramide levels	840:869	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	2	56	theme	inflammasome	291:302	arg1	activation					304:313	NLRP3 inflammasome activation	285:313	NLRP3 inflammasome activation	285:313	NLRP3 inflammasome activation may underlie obesity-induced inflammation and insulin resistance, and NLRP3 deficient mice exposed to high fat diet (HFD) appear to be protected from left ventricle (LV) concentric remodeling.
33273482	4	57	theme	NLRP3	815:819	arg1	mice					831:834	Obese NLRP3 deficient mice	809:834	Obese NLRP3 deficient mice	809:834	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	4	58	theme	proteomic	1150:1158	arg1	analyses					1160:1167	proteomic analyses	1150:1167	proteomic analyses of LV heart tissue	1150:1186	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	3	59	theme	content	725:731	arg1	sequencing					705:714	16S rRNA sequencing	696:714	16S rRNA sequencing of cecum content	696:731	Herein, we investigated if these beneficial effects were associated with alterations in plasma metabolites, using metabolomic and lipidomic analysis, and gut microbiota composition, using 16S rRNA sequencing of cecum content, comparing NLRP3 deficient and wild type (WT) mice on HFD and control diet.
33273482	4	60	theme	myocardial	1106:1115	arg1	metabolism					1124:1133	downregulated myocardial energy metabolism	1092:1133	downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue	1092:1186	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	3	61	theme	wild	764:767	arg1	WT					775:776	WT	775:776	WT	775:776	Herein, we investigated if these beneficial effects were associated with alterations in plasma metabolites, using metabolomic and lipidomic analysis, and gut microbiota composition, using 16S rRNA sequencing of cecum content, comparing NLRP3 deficient and wild type (WT) mice on HFD and control diet.
33273482	3	61	theme	wild	764:767	arg1	type					769:772	wild type	764:772	wild type (WT)	764:777	Herein, we investigated if these beneficial effects were associated with alterations in plasma metabolites, using metabolomic and lipidomic analysis, and gut microbiota composition, using 16S rRNA sequencing of cecum content, comparing NLRP3 deficient and wild type (WT) mice on HFD and control diet.
33273482	0	62	theme	high	134:137	arg1	diet					143:146	high fat diet	134:146	high fat diet	134:146	NLRP3 inflammasome deficiency attenuates metabolic disturbances involving alterations in the gut microbial profile in mice exposed to high fat diet.
33273482	2	63	theme	obesity-induced	328:342	arg1	inflammation					344:355	obesity-induced inflammation	328:355	obesity-induced inflammation	328:355	NLRP3 inflammasome activation may underlie obesity-induced inflammation and insulin resistance, and NLRP3 deficient mice exposed to high fat diet (HFD) appear to be protected from left ventricle (LV) concentric remodeling.
33273482	6	64	theme	inflammasome	1554:1565	arg1	role					1540:1543	a role	1538:1543	a role of NLRP3 inflammasome in the interface between metabolic and inflammatory stress	1538:1624	Our findings support a role of NLRP3 inflammasome in the interface between metabolic and inflammatory stress, involving an altered gut microbiota composition.
33273482	3	65	theme	deficient	750:758	arg1	mice					779:782	NLRP3 deficient and wild type (WT) mice	744:782	NLRP3 deficient and wild type (WT) mice	744:782	Herein, we investigated if these beneficial effects were associated with alterations in plasma metabolites, using metabolomic and lipidomic analysis, and gut microbiota composition, using 16S rRNA sequencing of cecum content, comparing NLRP3 deficient and wild type (WT) mice on HFD and control diet.
33273482	1	66	theme	cardiovascular	209:222	arg1	disorders					224:232	cardiovascular disorders	209:232	cardiovascular disorders	209:232	Obesity-related diseases (e.g. type 2 diabetes mellitus and cardiovascular disorders) represent an increasing health problem worldwide.
33273482	4	67	theme	altered	920:926	arg1	expression					936:945	altered hepatic expression	920:945	altered hepatic expression of fatty acids (FA) with lower mono-saturated FA	920:994	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	6	68	theme	altered	1640:1646	arg1	composition					1663:1673	an altered gut microbiota composition	1637:1673	an altered gut microbiota composition	1637:1673	Our findings support a role of NLRP3 inflammasome in the interface between metabolic and inflammatory stress, involving an altered gut microbiota composition.
33273482	4	69	theme	different	1193:1201	arg1	levels					1203:1208	different levels	1193:1208	different levels of bile acids	1193:1222	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	4	70	contain	had	836:838	arg1	mice					831:834	Obese NLRP3 deficient mice	809:834	Obese NLRP3 deficient mice	809:834	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	4	70	contain	had	836:838	arg2	levels					864:869	lower systemic ceramide levels	840:869	lower systemic ceramide levels	840:869	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	4	71	theme	heart	1175:1179	arg1	tissue					1181:1186	LV heart tissue	1172:1186	LV heart tissue	1172:1186	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	0	72	theme	microbial	97:105	arg1	profile					107:113	the gut microbial profile	89:113	the gut microbial profile in mice exposed to high fat diet	89:146	NLRP3 inflammasome deficiency attenuates metabolic disturbances involving alterations in the gut microbial profile in mice exposed to high fat diet.
33273482	2	73	dep	ventricle	470:478	arg1	remodeling					496:505	concentric remodeling	485:505	left ventricle (LV) concentric remodeling	465:505	NLRP3 inflammasome activation may underlie obesity-induced inflammation and insulin resistance, and NLRP3 deficient mice exposed to high fat diet (HFD) appear to be protected from left ventricle (LV) concentric remodeling.
33273482	0	74	from	alterations	74:84	arg1	profile					107:113	the gut microbial profile	89:113	the gut microbial profile in mice exposed to high fat diet	89:146	NLRP3 inflammasome deficiency attenuates metabolic disturbances involving alterations in the gut microbial profile in mice exposed to high fat diet.
33273482	4	75	theme	WT	1241:1242	arg1	mice					1244:1247	WT mice	1241:1247	WT mice	1241:1247	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	5	76	theme	microbiota	1314:1323	arg1	composition					1295:1305	an altered composition	1284:1305	an altered composition	1284:1305	These changes were accompanied by an altered composition of gut microbiota associated with decreased systemic levels of tri-methylamine-N-oxide and lipopolysaccharide, potentially inducing attenuating systemic inflammation and beneficial effects on lipid metabolism.
33273482	4	77	theme	fatty	950:954	arg1	FA					963:964	FA	963:964	FA	963:964	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	4	77	theme	fatty	950:954	arg1	acids					956:960	fatty acids	950:960	fatty acids (FA) with lower mono-saturated FA	950:994	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	2	78	theme	fat	422:424	arg1	HFD					432:434	HFD	432:434	HFD	432:434	NLRP3 inflammasome activation may underlie obesity-induced inflammation and insulin resistance, and NLRP3 deficient mice exposed to high fat diet (HFD) appear to be protected from left ventricle (LV) concentric remodeling.
33273482	2	78	theme	fat	422:424	arg1	diet					426:429	high fat diet	417:429	high fat diet (HFD)	417:435	NLRP3 inflammasome activation may underlie obesity-induced inflammation and insulin resistance, and NLRP3 deficient mice exposed to high fat diet (HFD) appear to be protected from left ventricle (LV) concentric remodeling.
33273482	0	79	theme	inflammasome	6:17	arg1	deficiency					19:28	NLRP3 inflammasome deficiency	0:28	NLRP3 inflammasome deficiency	0:28	NLRP3 inflammasome deficiency attenuates metabolic disturbances involving alterations in the gut microbial profile in mice exposed to high fat diet.
33273482	4	80	theme	higher	1000:1005	arg1	levels					1026:1031	higher polyunsaturated FA levels	1000:1031	higher polyunsaturated FA levels	1000:1031	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	4	81	theme	acids	1218:1222	arg1	levels					1203:1208	different levels	1193:1208	different levels of bile acids	1193:1222	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	4	81	theme	acids	1218:1222	arg1	development					1060:1070	development	1060:1070	development of liver steatosis	1060:1089	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	4	81	theme	acids	1218:1222	arg1	metabolism					1124:1133	downregulated myocardial energy metabolism	1092:1133	downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue	1092:1186	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	5	82	theme	altered	1287:1293	arg1	composition					1295:1305	an altered composition	1284:1305	an altered composition	1284:1305	These changes were accompanied by an altered composition of gut microbiota associated with decreased systemic levels of tri-methylamine-N-oxide and lipopolysaccharide, potentially inducing attenuating systemic inflammation and beneficial effects on lipid metabolism.
33273482	6	83	theme	microbiota	1652:1661	arg1	composition					1663:1673	an altered gut microbiota composition	1637:1673	an altered gut microbiota composition	1637:1673	Our findings support a role of NLRP3 inflammasome in the interface between metabolic and inflammatory stress, involving an altered gut microbiota composition.
33273482	4	84	theme	FA	1023:1024	arg1	levels					1026:1031	higher polyunsaturated FA levels	1000:1031	higher polyunsaturated FA levels	1000:1031	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	4	85	theme	lower	972:976	arg1	FA					993:994	lower mono-saturated FA	972:994	lower mono-saturated FA	972:994	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	6	86	from	role	1540:1543	arg1	interface					1574:1582	the interface	1570:1582	the interface between metabolic and inflammatory stress	1570:1624	Our findings support a role of NLRP3 inflammasome in the interface between metabolic and inflammatory stress, involving an altered gut microbiota composition.
33273482	2	87	theme	left	465:468	arg1	ventricle					470:478	left ventricle	465:478	left ventricle (LV) concentric remodeling	465:505	NLRP3 inflammasome activation may underlie obesity-induced inflammation and insulin resistance, and NLRP3 deficient mice exposed to high fat diet (HFD) appear to be protected from left ventricle (LV) concentric remodeling.
33273482	2	87	theme	left	465:468	arg1	LV					481:482	LV	481:482	LV	481:482	NLRP3 inflammasome activation may underlie obesity-induced inflammation and insulin resistance, and NLRP3 deficient mice exposed to high fat diet (HFD) appear to be protected from left ventricle (LV) concentric remodeling.
33273482	3	88	theme	microbiota	666:675	arg1	composition					677:687	gut microbiota composition	662:687	gut microbiota composition	662:687	Herein, we investigated if these beneficial effects were associated with alterations in plasma metabolites, using metabolomic and lipidomic analysis, and gut microbiota composition, using 16S rRNA sequencing of cecum content, comparing NLRP3 deficient and wild type (WT) mice on HFD and control diet.
33273482	6	89	theme	gut	1648:1650	arg1	composition					1663:1673	an altered gut microbiota composition	1637:1673	an altered gut microbiota composition	1637:1673	Our findings support a role of NLRP3 inflammasome in the interface between metabolic and inflammatory stress, involving an altered gut microbiota composition.
33273482	5	90	theme	systemic	1351:1358	arg1	levels					1360:1365	decreased systemic levels	1341:1365	decreased systemic levels of tri-methylamine-N-oxide and lipopolysaccharide	1341:1415	These changes were accompanied by an altered composition of gut microbiota associated with decreased systemic levels of tri-methylamine-N-oxide and lipopolysaccharide, potentially inducing attenuating systemic inflammation and beneficial effects on lipid metabolism.
33273482	2	91	theme	insulin	361:367	arg1	resistance					369:378	insulin resistance	361:378	insulin resistance	361:378	NLRP3 inflammasome activation may underlie obesity-induced inflammation and insulin resistance, and NLRP3 deficient mice exposed to high fat diet (HFD) appear to be protected from left ventricle (LV) concentric remodeling.
33273482	5	92	from	effects	1488:1494	arg1	metabolism					1505:1514	lipid metabolism	1499:1514	lipid metabolism	1499:1514	These changes were accompanied by an altered composition of gut microbiota associated with decreased systemic levels of tri-methylamine-N-oxide and lipopolysaccharide, potentially inducing attenuating systemic inflammation and beneficial effects on lipid metabolism.
33273482	4	93	theme	steatosis	1081:1089	arg1	levels					1203:1208	different levels	1193:1208	different levels of bile acids	1193:1222	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	4	93	theme	steatosis	1081:1089	arg1	development					1060:1070	development	1060:1070	development of liver steatosis	1060:1089	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	4	93	theme	steatosis	1081:1089	arg1	metabolism					1124:1133	downregulated myocardial energy metabolism	1092:1133	downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue	1092:1186	Obese NLRP3 deficient mice had lower systemic ceramide levels, potentially resulting attenuating inflammation, altered hepatic expression of fatty acids (FA) with lower mono-saturated FA and higher polyunsaturated FA levels, potentially counteracting development of liver steatosis, downregulated myocardial energy metabolism as assessed by proteomic analyses of LV heart tissue, and different levels of bile acids as compared with WT mice.
33273482	1	94	theme	increasing	248:257	arg1	worldwide					274:282	an increasing health problem worldwide	245:282	an increasing health problem worldwide	245:282	Obesity-related diseases (e.g. type 2 diabetes mellitus and cardiovascular disorders) represent an increasing health problem worldwide.
32401138	13	0	theme	fiber	1894:1898	arg1	fermentation					1900:1911	fiber fermentation	1894:1911	fiber fermentation	1894:1911	Taxons enriched in athletes are known to participate in fiber fermentation.
32401138	11	1	theme	fiber	1688:1692	arg1	amount					1694:1699	fiber amount	1688:1699	fiber amount in diet	1688:1707	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	12	2	theme	diversity	1827:1835	arg1	promotion					1797:1805	promotion	1797:1805	promotion	1797:1805	CONCLUSIONS The excessive training associates with both differences in composition and promotion of higher bacterial diversity.
32401138	12	2	theme	diversity	1827:1835	arg1	composition					1781:1791	composition	1781:1791	composition	1781:1791	CONCLUSIONS The excessive training associates with both differences in composition and promotion of higher bacterial diversity.
32401138	4	3	theme	marathon	614:621	arg1	runners					623:629	14 marathon runners	611:629	14 marathon runners	611:629	MATERIALS AND METHODS Stools samples were collected from 14 marathon runners, 11 cross-country skiers and 46 sedentary healthy controls.
32401138	11	4	theme	fatty	1576:1580	arg1	acids					1582:1586	polyunsaturated fatty acids	1560:1586	polyunsaturated fatty acids	1560:1586	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	8	5	theme	differentiated	997:1010	arg1	controls					1020:1027	differentiated healthy controls	997:1027	differentiated healthy controls	997:1027	RESULTS 20 and 5 taxa differentiated healthy controls from marathon runners and cross-country skiers, respectively.
32401138	10	6	from	skiers	1278:1283	arg1	diverse					1253:1259	diverse	1253:1259	diverse	1253:1259	The athletes' microbiome was also more diverse in cross-country skiers than the one of sedentary controls (Simpson index p-value at 0.025).
32401138	11	7	theme	strong	1365:1370	arg1	>					1409:1409	Spearman's coefficient > 0.6	1386:1413	Spearman's coefficient > 0.6	1386:1413	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	11	7	theme	strong	1365:1370	arg1	correlations					1372:1383	Thirty-one strong correlations	1354:1383	Thirty-one strong correlations (Spearman's coefficient > 0.6)	1354:1414	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	6	8	theme	16S	790:792	arg1	sequencing					799:808	The 16S gene sequencing	786:808	The 16S gene sequencing	786:808	The 16S gene sequencing was performed using the Ion 16S Metagenomics Kit on Ion Torrent PGM sequencer.
32401138	2	9	theme	most	306:309	arg1	studies					311:317	most studies	306:317	most studies	306:317	However, most studies suggest that athletes' microbiota undergoes major alterations, which may contribute to increased physical performance.
32401138	1	10	theme	gut	179:181	arg1	composition					194:204	gut microbiota composition	179:204	gut microbiota composition	179:204	BACKGROUND Little data are available on the subject of gut microbiota composition in endurance athletes as well as connections between diet and specific bacteria abundance.
32401138	8	11	theme	marathon	1034:1041	arg1	runners					1043:1049	marathon runners	1034:1049	marathon runners	1034:1049	RESULTS 20 and 5 taxa differentiated healthy controls from marathon runners and cross-country skiers, respectively.
32401138	1	12	theme	composition	194:204	arg1	subject					168:174	the subject	164:174	the subject of gut microbiota composition in endurance athletes as well as connections between diet and specific bacteria abundance	164:294	BACKGROUND Little data are available on the subject of gut microbiota composition in endurance athletes as well as connections between diet and specific bacteria abundance.
32401138	4	13	theme	sedentary	663:671	arg1	controls					681:688	46 sedentary healthy controls	660:688	46 sedentary healthy controls	660:688	MATERIALS AND METHODS Stools samples were collected from 14 marathon runners, 11 cross-country skiers and 46 sedentary healthy controls.
32401138	5	14	theme	24-h	740:743	arg1	recall					750:755	24-h diet recall	740:755	24-h diet recall	740:755	The athletes' diet evaluation was performed with 24-h diet recall, using the Aliant programme.
32401138	1	15	theme	endurance	209:217	arg1	athletes					219:226	endurance athletes	209:226	endurance athletes as well as connections between diet and specific bacteria abundance	209:294	BACKGROUND Little data are available on the subject of gut microbiota composition in endurance athletes as well as connections between diet and specific bacteria abundance.
32401138	10	16	theme	cross-country	1264:1276	arg1	skiers					1278:1283	cross-country skiers	1264:1283	cross-country skiers than the one of sedentary controls (Simpson index p-value at 0.025)	1264:1351	The athletes' microbiome was also more diverse in cross-country skiers than the one of sedentary controls (Simpson index p-value at 0.025).
32401138	10	17	dep	controls	1311:1318	arg1	index					1329:1333	Simpson index	1321:1333	Simpson index p-value at 0.025	1321:1350	The athletes' microbiome was also more diverse in cross-country skiers than the one of sedentary controls (Simpson index p-value at 0.025).
32401138	1	18	theme	Little	135:140	arg1	data					142:145	BACKGROUND Little data	124:145	BACKGROUND Little data	124:145	BACKGROUND Little data are available on the subject of gut microbiota composition in endurance athletes as well as connections between diet and specific bacteria abundance.
32401138	10	19	from	0.025	1346:1350	arg1	p-value					1335:1341	p-value	1335:1341	p-value	1335:1341	The athletes' microbiome was also more diverse in cross-country skiers than the one of sedentary controls (Simpson index p-value at 0.025).
32401138	5	20	theme	Aliant	768:773	arg1	programme					775:783	the Aliant programme	764:783	the Aliant programme	764:783	The athletes' diet evaluation was performed with 24-h diet recall, using the Aliant programme.
32401138	11	21	theme	polyunsaturated	1560:1574	arg1	acids					1582:1586	polyunsaturated fatty acids	1560:1586	polyunsaturated fatty acids	1560:1586	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	0	22	theme	top	69:71	arg1	athletes					90:97	the top Polish endurance athletes	65:97	the top Polish endurance athletes from sedentary controls	65:121	The composition and richness of the gut microbiota differentiate the top Polish endurance athletes from sedentary controls.
32401138	0	23	theme	endurance	80:88	arg1	athletes					90:97	the top Polish endurance athletes	65:97	the top Polish endurance athletes from sedentary controls	65:121	The composition and richness of the gut microbiota differentiate the top Polish endurance athletes from sedentary controls.
32401138	9	24	theme	gut	1142:1144	arg1	Bacteroidetes					1164:1176	Bacteroidetes	1164:1176	Bacteroidetes	1164:1176	Both groups presented a lowered abundance of major gut microbiota genus, Bacteroidetes and higher abundance of Prevotella.
32401138	9	24	theme	gut	1142:1144	arg1	genus					1157:1161	major gut microbiota genus	1136:1161	major gut microbiota genus	1136:1161	Both groups presented a lowered abundance of major gut microbiota genus, Bacteroidetes and higher abundance of Prevotella.
32401138	12	25	theme	excessive	1726:1734	arg1	training					1736:1743	The excessive training	1722:1743	The excessive training	1722:1743	CONCLUSIONS The excessive training associates with both differences in composition and promotion of higher bacterial diversity.
32401138	10	26	theme	sedentary	1301:1309	arg1	controls					1311:1318	sedentary controls	1301:1318	sedentary controls (Simpson index p-value at 0.025)	1301:1351	The athletes' microbiome was also more diverse in cross-country skiers than the one of sedentary controls (Simpson index p-value at 0.025).
32401138	1	27	theme	specific	268:275	arg1	bacteria					277:284	specific bacteria	268:284	specific bacteria	268:284	BACKGROUND Little data are available on the subject of gut microbiota composition in endurance athletes as well as connections between diet and specific bacteria abundance.
32401138	4	28	theme	METHODS	568:574	arg1	samples					583:589	MATERIALS AND METHODS Stools samples	554:589	MATERIALS AND METHODS Stools samples	554:589	MATERIALS AND METHODS Stools samples were collected from 14 marathon runners, 11 cross-country skiers and 46 sedentary healthy controls.
32401138	9	29	theme	major	1136:1140	arg1	Bacteroidetes					1164:1176	Bacteroidetes	1164:1176	Bacteroidetes	1164:1176	Both groups presented a lowered abundance of major gut microbiota genus, Bacteroidetes and higher abundance of Prevotella.
32401138	9	29	theme	major	1136:1140	arg1	genus					1157:1161	major gut microbiota genus	1136:1161	major gut microbiota genus	1136:1161	Both groups presented a lowered abundance of major gut microbiota genus, Bacteroidetes and higher abundance of Prevotella.
32401138	6	30	theme	PGM	874:876	arg1	sequencer					878:886	Ion Torrent PGM sequencer	862:886	Ion Torrent PGM sequencer	862:886	The 16S gene sequencing was performed using the Ion 16S Metagenomics Kit on Ion Torrent PGM sequencer.
32401138	9	31	theme	microbiota	1146:1155	arg1	Bacteroidetes					1164:1176	Bacteroidetes	1164:1176	Bacteroidetes	1164:1176	Both groups presented a lowered abundance of major gut microbiota genus, Bacteroidetes and higher abundance of Prevotella.
32401138	9	31	theme	microbiota	1146:1155	arg1	genus					1157:1161	major gut microbiota genus	1136:1161	major gut microbiota genus	1136:1161	Both groups presented a lowered abundance of major gut microbiota genus, Bacteroidetes and higher abundance of Prevotella.
32401138	11	32	theme	inverse	1478:1484	arg1	correlation					1486:1496	inverse correlation	1478:1496	inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet	1478:1707	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	2	33	theme	increased	406:414	arg1	performance					425:435	increased physical performance	406:435	increased physical performance	406:435	However, most studies suggest that athletes' microbiota undergoes major alterations, which may contribute to increased physical performance.
32401138	6	34	theme	Ion	862:864	arg1	sequencer					878:886	Ion Torrent PGM sequencer	862:886	Ion Torrent PGM sequencer	862:886	The 16S gene sequencing was performed using the Ion 16S Metagenomics Kit on Ion Torrent PGM sequencer.
32401138	11	35	with	acids	1582:1586	arg1	intake					1525:1530	sucrose intake	1517:1530	sucrose intake	1517:1530	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	11	35	with	acids	1582:1586	arg1	amount					1694:1699	fiber amount	1688:1699	fiber amount in diet	1688:1707	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	11	36	theme	acid	1654:1657	arg1	intake					1659:1664	folic acid intake	1648:1664	folic acid intake	1648:1664	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	0	37	theme	gut	36:38	arg1	microbiota					40:49	the gut microbiota	32:49	the gut microbiota	32:49	The composition and richness of the gut microbiota differentiate the top Polish endurance athletes from sedentary controls.
32401138	4	38	theme	MATERIALS	554:562	arg1	samples					583:589	MATERIALS AND METHODS Stools samples	554:589	MATERIALS AND METHODS Stools samples	554:589	MATERIALS AND METHODS Stools samples were collected from 14 marathon runners, 11 cross-country skiers and 46 sedentary healthy controls.
32401138	3	39	from	differences	475:485	arg1	microbiota					494:503	gut microbiota	490:503	gut microbiota	490:503	Therefore, we decided to investigate differences in gut microbiota between healthy controls and endurance athletes.
32401138	6	40	theme	16S	838:840	arg1	Kit					855:857	the Ion 16S Metagenomics Kit	830:857	the Ion 16S Metagenomics Kit on Ion Torrent PGM sequencer	830:886	The 16S gene sequencing was performed using the Ion 16S Metagenomics Kit on Ion Torrent PGM sequencer.
32401138	9	41	theme	genus	1157:1161	arg1	abundance					1189:1197	higher abundance	1182:1197	higher abundance of Prevotella	1182:1211	Both groups presented a lowered abundance of major gut microbiota genus, Bacteroidetes and higher abundance of Prevotella.
32401138	9	41	theme	genus	1157:1161	arg1	abundance					1123:1131	a lowered abundance	1113:1131	a lowered abundance of major gut microbiota genus, Bacteroidetes	1113:1176	Both groups presented a lowered abundance of major gut microbiota genus, Bacteroidetes and higher abundance of Prevotella.
32401138	7	42	theme	diversity	918:926	arg1	indices					928:934	diversity indices	918:934	diversity indices	918:934	Taxonomic classification and diversity indices computation was performed with Mothur.
32401138	0	43	from	controls	114:121	arg1	athletes					90:97	the top Polish endurance athletes	65:97	the top Polish endurance athletes from sedentary controls	65:121	The composition and richness of the gut microbiota differentiate the top Polish endurance athletes from sedentary controls.
32401138	11	44	theme	coefficient	1397:1407	arg1	>					1409:1409	Spearman's coefficient > 0.6	1386:1413	Spearman's coefficient > 0.6	1386:1413	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	11	44	theme	coefficient	1397:1407	arg1	correlations					1372:1383	Thirty-one strong correlations	1354:1383	Thirty-one strong correlations (Spearman's coefficient > 0.6)	1354:1414	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	9	45	theme	Prevotella	1202:1211	arg1	abundance					1189:1197	higher abundance	1182:1197	higher abundance of Prevotella	1182:1211	Both groups presented a lowered abundance of major gut microbiota genus, Bacteroidetes and higher abundance of Prevotella.
32401138	9	45	theme	Prevotella	1202:1211	arg1	abundance					1123:1131	a lowered abundance	1113:1131	a lowered abundance of major gut microbiota genus, Bacteroidetes	1113:1176	Both groups presented a lowered abundance of major gut microbiota genus, Bacteroidetes and higher abundance of Prevotella.
32401138	3	46	theme	healthy	513:519	arg1	controls					521:528	healthy controls	513:528	healthy controls	513:528	Therefore, we decided to investigate differences in gut microbiota between healthy controls and endurance athletes.
32401138	7	47	theme	classification	899:912	arg1	computation					936:946	Taxonomic classification and diversity indices computation	889:946	Taxonomic classification and diversity indices computation	889:946	Taxonomic classification and diversity indices computation was performed with Mothur.
32401138	8	48	theme	healthy	1012:1018	arg1	controls					1020:1027	differentiated healthy controls	997:1027	differentiated healthy controls	997:1027	RESULTS 20 and 5 taxa differentiated healthy controls from marathon runners and cross-country skiers, respectively.
32401138	10	49	from	diverse	1253:1259	arg1	skiers					1278:1283	cross-country skiers	1264:1283	cross-country skiers than the one of sedentary controls (Simpson index p-value at 0.025)	1264:1351	The athletes' microbiome was also more diverse in cross-country skiers than the one of sedentary controls (Simpson index p-value at 0.025).
32401138	4	50	theme	healthy	673:679	arg1	controls					681:688	46 sedentary healthy controls	660:688	46 sedentary healthy controls	660:688	MATERIALS AND METHODS Stools samples were collected from 14 marathon runners, 11 cross-country skiers and 46 sedentary healthy controls.
32401138	11	51	theme	Thirty-one	1354:1363	arg1	>					1409:1409	Spearman's coefficient > 0.6	1386:1413	Spearman's coefficient > 0.6	1386:1413	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	11	51	theme	Thirty-one	1354:1363	arg1	correlations					1372:1383	Thirty-one strong correlations	1354:1383	Thirty-one strong correlations (Spearman's coefficient > 0.6)	1354:1414	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	6	52	theme	gene	794:797	arg1	sequencing					799:808	The 16S gene sequencing	786:808	The 16S gene sequencing	786:808	The 16S gene sequencing was performed using the Ion 16S Metagenomics Kit on Ion Torrent PGM sequencer.
32401138	12	53	theme	bacterial	1817:1825	arg1	diversity					1827:1835	higher bacterial diversity	1810:1835	higher bacterial diversity	1810:1835	CONCLUSIONS The excessive training associates with both differences in composition and promotion of higher bacterial diversity.
32401138	11	54	theme	positive	1599:1606	arg1	correlation					1608:1618	positive correlation	1599:1618	positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet	1599:1707	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	1	55	theme	microbiota	183:192	arg1	composition					194:204	gut microbiota composition	179:204	gut microbiota composition	179:204	BACKGROUND Little data are available on the subject of gut microbiota composition in endurance athletes as well as connections between diet and specific bacteria abundance.
32401138	11	56	with	correlation	1486:1496	arg1	correlation					1608:1618	positive correlation	1599:1618	positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet	1599:1707	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	11	56	with	correlation	1486:1496	arg1	acids					1582:1586	polyunsaturated fatty acids	1560:1586	polyunsaturated fatty acids	1560:1586	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	6	57	from	Kit	855:857	arg1	sequencer					878:886	Ion Torrent PGM sequencer	862:886	Ion Torrent PGM sequencer	862:886	The 16S gene sequencing was performed using the Ion 16S Metagenomics Kit on Ion Torrent PGM sequencer.
32401138	1	58	dep	diet	259:262	arg1	abundance					286:294	abundance	286:294	abundance	286:294	BACKGROUND Little data are available on the subject of gut microbiota composition in endurance athletes as well as connections between diet and specific bacteria abundance.
32401138	12	59	from	differences	1766:1776	arg1	promotion					1797:1805	promotion	1797:1805	promotion	1797:1805	CONCLUSIONS The excessive training associates with both differences in composition and promotion of higher bacterial diversity.
32401138	12	59	from	differences	1766:1776	arg1	composition					1781:1791	composition	1781:1791	composition	1781:1791	CONCLUSIONS The excessive training associates with both differences in composition and promotion of higher bacterial diversity.
32401138	11	60	theme	sucrose	1517:1523	arg1	intake					1525:1530	sucrose intake	1517:1530	sucrose intake	1517:1530	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	12	61	theme	higher	1810:1815	arg1	diversity					1827:1835	higher bacterial diversity	1810:1835	higher bacterial diversity	1810:1835	CONCLUSIONS The excessive training associates with both differences in composition and promotion of higher bacterial diversity.
32401138	5	62	theme	diet	705:708	arg1	evaluation					710:719	The athletes' diet evaluation	691:719	The athletes' diet evaluation	691:719	The athletes' diet evaluation was performed with 24-h diet recall, using the Aliant programme.
32401138	1	63	theme	BACKGROUND	124:133	arg1	data					142:145	BACKGROUND Little data	124:145	BACKGROUND Little data	124:145	BACKGROUND Little data are available on the subject of gut microbiota composition in endurance athletes as well as connections between diet and specific bacteria abundance.
32401138	11	64	dep	bacteria	1439:1446	arg1	diet					1462:1465	diet	1462:1465	diet	1462:1465	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	11	64	dep	bacteria	1439:1446	arg1	abundance					1448:1456	abundance	1448:1456	abundance	1448:1456	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	11	64	dep	bacteria	1439:1446	arg1	correlation					1486:1496	inverse correlation	1478:1496	inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet	1478:1707	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	11	64	dep	bacteria	1439:1446	arg1	bacteria					1439:1446	bacteria abundance and diet	1439:1465	bacteria abundance and diet	1439:1465	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	11	65	theme	Prevotella	1501:1510	arg1	correlation					1486:1496	inverse correlation	1478:1496	inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet	1478:1707	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	0	66	theme	Polish	73:78	arg1	athletes					90:97	the top Polish endurance athletes	65:97	the top Polish endurance athletes from sedentary controls	65:121	The composition and richness of the gut microbiota differentiate the top Polish endurance athletes from sedentary controls.
32401138	4	67	dep	MATERIALS	554:562	arg1	Stools					576:581	Stools	576:581	Stools	576:581	MATERIALS AND METHODS Stools samples were collected from 14 marathon runners, 11 cross-country skiers and 46 sedentary healthy controls.
32401138	8	68	theme	cross-country	1055:1067	arg1	skiers					1069:1074	cross-country skiers	1055:1074	cross-country skiers	1055:1074	RESULTS 20 and 5 taxa differentiated healthy controls from marathon runners and cross-country skiers, respectively.
32401138	1	69	from	subject	168:174	arg1	athletes					219:226	endurance athletes	209:226	endurance athletes as well as connections between diet and specific bacteria abundance	209:294	BACKGROUND Little data are available on the subject of gut microbiota composition in endurance athletes as well as connections between diet and specific bacteria abundance.
32401138	1	69	from	subject	168:174	arg1	connections					239:249	connections	239:249	endurance athletes as well as connections between diet and specific bacteria abundance	209:294	BACKGROUND Little data are available on the subject of gut microbiota composition in endurance athletes as well as connections between diet and specific bacteria abundance.
32401138	5	70	theme	diet	745:748	arg1	recall					750:755	24-h diet recall	740:755	24-h diet recall	740:755	The athletes' diet evaluation was performed with 24-h diet recall, using the Aliant programme.
32401138	11	71	theme	Agathobacter	1670:1681	arg1	correlation					1608:1618	positive correlation	1599:1618	positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet	1599:1707	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	11	71	theme	Agathobacter	1670:1681	arg1	acids					1582:1586	polyunsaturated fatty acids	1560:1586	polyunsaturated fatty acids	1560:1586	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	10	72	theme	Simpson	1321:1327	arg1	index					1329:1333	Simpson index	1321:1333	Simpson index p-value at 0.025	1321:1350	The athletes' microbiome was also more diverse in cross-country skiers than the one of sedentary controls (Simpson index p-value at 0.025).
32401138	0	73	theme	sedentary	104:112	arg1	controls					114:121	sedentary controls	104:121	sedentary controls	104:121	The composition and richness of the gut microbiota differentiate the top Polish endurance athletes from sedentary controls.
32401138	9	74	theme	lowered	1115:1121	arg1	abundance					1123:1131	a lowered abundance	1113:1131	a lowered abundance of major gut microbiota genus, Bacteroidetes	1113:1176	Both groups presented a lowered abundance of major gut microbiota genus, Bacteroidetes and higher abundance of Prevotella.
32401138	11	75	with	correlation	1608:1618	arg1	intake					1525:1530	sucrose intake	1517:1530	sucrose intake	1517:1530	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	11	75	with	correlation	1608:1618	arg1	amount					1694:1699	fiber amount	1688:1699	fiber amount in diet	1688:1707	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	10	76	theme	p-value	1335:1341	arg1	index					1329:1333	Simpson index	1321:1333	Simpson index p-value at 0.025	1321:1350	The athletes' microbiome was also more diverse in cross-country skiers than the one of sedentary controls (Simpson index p-value at 0.025).
32401138	2	77	theme	physical	416:423	arg1	performance					425:435	increased physical performance	406:435	increased physical performance	406:435	However, most studies suggest that athletes' microbiota undergoes major alterations, which may contribute to increased physical performance.
32401138	6	78	theme	Torrent	866:872	arg1	sequencer					878:886	Ion Torrent PGM sequencer	862:886	Ion Torrent PGM sequencer	862:886	The 16S gene sequencing was performed using the Ion 16S Metagenomics Kit on Ion Torrent PGM sequencer.
32401138	11	79	from	amount	1694:1699	arg1	diet					1704:1707	diet	1704:1707	diet	1704:1707	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	11	80	theme	folic	1648:1652	arg1	intake					1659:1664	folic acid intake	1648:1664	folic acid intake	1648:1664	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	0	81	theme	microbiota	40:49	arg1	richness					20:27	richness	20:27	richness	20:27	The composition and richness of the gut microbiota differentiate the top Polish endurance athletes from sedentary controls.
32401138	0	81	theme	microbiota	40:49	arg1	composition					4:14	composition	4:14	composition	4:14	The composition and richness of the gut microbiota differentiate the top Polish endurance athletes from sedentary controls.
32401138	2	82	theme	major	363:367	arg1	alterations					369:379	major alterations	363:379	major alterations	363:379	However, most studies suggest that athletes' microbiota undergoes major alterations, which may contribute to increased physical performance.
32401138	11	83	theme	Christensenellaceae	1623:1641	arg1	correlation					1608:1618	positive correlation	1599:1618	positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet	1599:1707	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	11	83	theme	Christensenellaceae	1623:1641	arg1	acids					1582:1586	polyunsaturated fatty acids	1560:1586	polyunsaturated fatty acids	1560:1586	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	7	84	theme	indices	928:934	arg1	computation					936:946	Taxonomic classification and diversity indices computation	889:946	Taxonomic classification and diversity indices computation	889:946	Taxonomic classification and diversity indices computation was performed with Mothur.
32401138	6	85	theme	Metagenomics	842:853	arg1	Kit					855:857	the Ion 16S Metagenomics Kit	830:857	the Ion 16S Metagenomics Kit on Ion Torrent PGM sequencer	830:886	The 16S gene sequencing was performed using the Ion 16S Metagenomics Kit on Ion Torrent PGM sequencer.
32401138	11	86	dep	acids	1582:1586	arg1	Phascolarctobacterium					1533:1553	Phascolarctobacterium	1533:1553	Phascolarctobacterium	1533:1553	Thirty-one strong correlations (Spearman's coefficient > 0.6) were uncovered between bacteria abundance and diet, including inverse correlation of Prevotella with sucrose intake, Phascolarctobacterium with polyunsaturated fatty acids as well as positive correlation of Christensenellaceae with folic acid intake and Agathobacter with fiber amount in diet.
32401138	9	87	theme	higher	1182:1187	arg1	abundance					1189:1197	higher abundance	1182:1197	higher abundance of Prevotella	1182:1211	Both groups presented a lowered abundance of major gut microbiota genus, Bacteroidetes and higher abundance of Prevotella.
32401138	3	88	theme	gut	490:492	arg1	microbiota					494:503	gut microbiota	490:503	gut microbiota	490:503	Therefore, we decided to investigate differences in gut microbiota between healthy controls and endurance athletes.
32401138	10	89	theme	controls	1311:1318	arg1	one					1294:1296	one	1294:1296	one	1294:1296	The athletes' microbiome was also more diverse in cross-country skiers than the one of sedentary controls (Simpson index p-value at 0.025).
32401138	10	89	theme	controls	1311:1318	arg1	controls					1311:1318	sedentary controls	1301:1318	sedentary controls (Simpson index p-value at 0.025)	1301:1351	The athletes' microbiome was also more diverse in cross-country skiers than the one of sedentary controls (Simpson index p-value at 0.025).
32401138	6	90	theme	Ion	834:836	arg1	Kit					855:857	the Ion 16S Metagenomics Kit	830:857	the Ion 16S Metagenomics Kit on Ion Torrent PGM sequencer	830:886	The 16S gene sequencing was performed using the Ion 16S Metagenomics Kit on Ion Torrent PGM sequencer.
32401138	0	91	dep	composition	4:14	arg1	The					0:2	The	0:2	The	0:2	The composition and richness of the gut microbiota differentiate the top Polish endurance athletes from sedentary controls.
32401138	4	92	theme	cross-country	635:647	arg1	skiers					649:654	11 cross-country skiers	632:654	11 cross-country skiers	632:654	MATERIALS AND METHODS Stools samples were collected from 14 marathon runners, 11 cross-country skiers and 46 sedentary healthy controls.
32401138	12	93	dep	CONCLUSIONS	1710:1720	arg1	associates					1745:1754	associates	1745:1754	associates with both differences in composition and promotion of higher bacterial diversity	1745:1835	CONCLUSIONS The excessive training associates with both differences in composition and promotion of higher bacterial diversity.
32401138	3	94	theme	endurance	534:542	arg1	athletes					544:551	endurance athletes	534:551	endurance athletes	534:551	Therefore, we decided to investigate differences in gut microbiota between healthy controls and endurance athletes.
32401138	7	95	theme	Taxonomic	889:897	arg1	classification					899:912	Taxonomic classification	889:912	Taxonomic classification	889:912	Taxonomic classification and diversity indices computation was performed with Mothur.
32544578	0	0	theme	antimicrobial	124:136	arg1	properties					138:147	photocatalytic and antimicrobial properties	105:147	photocatalytic and antimicrobial properties under ultra-violet irradiation	105:178	Synthesis of Co3S4-SnO2/polyvinylpyrrolidone-cellulose heterojunction as highly performance catalyst for photocatalytic and antimicrobial properties under ultra-violet irradiation.
32544578	4	1	theme	light	601:605	arg1	irradiation					607:617	ultra-violet (UV) light irradiation	583:617	ultra-violet (UV) light irradiation	583:617	Moreover, the photocatalysis performances of the catalysts were investigated under ultra-violet (UV) light irradiation.
32544578	4	2	theme	UV	597:598	arg1	irradiation					607:617	ultra-violet (UV) light irradiation	583:617	ultra-violet (UV) light irradiation	583:617	Moreover, the photocatalysis performances of the catalysts were investigated under ultra-violet (UV) light irradiation.
32544578	8	3	theme	composite	1061:1069	arg1	properties					1030:1039	The biological properties	1015:1039	The biological properties of Co3S4-SnO2/PVPCS composite	1015:1069	The biological properties of Co3S4-SnO2/PVPCS composite were investigated through the antibacterial (versus Staphylococcus aureus, and Escherichia coli) and antifungal studies (Candida albicans).
32544578	1	4	theme	Co3S4-SnO2	206:215	arg1	nano-structure					266:279	Co3S4-SnO2 supported polyvinylpyrrolidone-cellulose (PVPCS) nano-structure	206:279	Co3S4-SnO2 supported polyvinylpyrrolidone-cellulose (PVPCS) nano-structure for Lidocaine degradation	206:305	In this work, we present Co3S4-SnO2 supported polyvinylpyrrolidone-cellulose (PVPCS) nano-structure for Lidocaine degradation.
32544578	9	5	theme	biological	1279:1288	arg1	properties					1290:1299	substantial biological properties	1267:1299	substantial biological properties	1267:1299	As the results declared, Co3S4-SnO2 nanocomposites have substantial biological properties as compared to SnO2 nanoparticles, and Co3S4-SnO2 nanocomposites.
32544578	2	6	theme	morphological	371:383	arg1	ones					397:400	morphological and optical ones	371:400	morphological and optical ones	371:400	The nanostructure was characterized by various techniques i.e. morphological and optical ones.
32544578	3	7	theme	Co3S4-SnO2	438:447	arg1	nanocomposites					449:462	Co3S4-SnO2 nanocomposites	438:462	Co3S4-SnO2 nanocomposites	438:462	The results have demonstrated that Co3S4-SnO2 nanocomposites were evenly supported on the PVPCS.
32544578	4	8	theme	ultra-violet	583:594	arg1	irradiation					607:617	ultra-violet (UV) light irradiation	583:617	ultra-violet (UV) light irradiation	583:617	Moreover, the photocatalysis performances of the catalysts were investigated under ultra-violet (UV) light irradiation.
32544578	5	9	theme	SnO2	738:741	arg1	nanoparticles					743:755	SnO2 nanoparticles	738:755	SnO2 nanoparticles	738:755	The nano-structure Co3S4-SnO2/PVPCS composite (98.72%) revealed the highest photocatalysis performance as compared to SnO2 nanoparticles, and Co3S4-SnO2 nanocomposites.
32544578	9	10	theme	SnO2	1316:1319	arg1	nanoparticles					1321:1333	SnO2 nanoparticles	1316:1333	SnO2 nanoparticles	1316:1333	As the results declared, Co3S4-SnO2 nanocomposites have substantial biological properties as compared to SnO2 nanoparticles, and Co3S4-SnO2 nanocomposites.
32544578	1	11	theme	supported	217:225	arg1	nano-structure					266:279	Co3S4-SnO2 supported polyvinylpyrrolidone-cellulose (PVPCS) nano-structure	206:279	Co3S4-SnO2 supported polyvinylpyrrolidone-cellulose (PVPCS) nano-structure for Lidocaine degradation	206:305	In this work, we present Co3S4-SnO2 supported polyvinylpyrrolidone-cellulose (PVPCS) nano-structure for Lidocaine degradation.
32544578	0	12	theme	ultra-violet	155:166	arg1	irradiation					168:178	ultra-violet irradiation	155:178	ultra-violet irradiation	155:178	Synthesis of Co3S4-SnO2/polyvinylpyrrolidone-cellulose heterojunction as highly performance catalyst for photocatalytic and antimicrobial properties under ultra-violet irradiation.
32544578	1	13	theme	polyvinylpyrrolidone-cellulose	227:256	arg1	nano-structure					266:279	Co3S4-SnO2 supported polyvinylpyrrolidone-cellulose (PVPCS) nano-structure	206:279	Co3S4-SnO2 supported polyvinylpyrrolidone-cellulose (PVPCS) nano-structure for Lidocaine degradation	206:305	In this work, we present Co3S4-SnO2 supported polyvinylpyrrolidone-cellulose (PVPCS) nano-structure for Lidocaine degradation.
32544578	1	14	attach	present	198:204	arg2	we					195:196	we	195:196	we	195:196	In this work, we present Co3S4-SnO2 supported polyvinylpyrrolidone-cellulose (PVPCS) nano-structure for Lidocaine degradation.
32544578	1	14	attach	present	198:204	arg1	work					189:192	this work	184:192	this work	184:192	In this work, we present Co3S4-SnO2 supported polyvinylpyrrolidone-cellulose (PVPCS) nano-structure for Lidocaine degradation.
32544578	5	15	theme	highest	688:694	arg1	performance					711:721	the highest photocatalysis performance	684:721	the highest photocatalysis performance	684:721	The nano-structure Co3S4-SnO2/PVPCS composite (98.72%) revealed the highest photocatalysis performance as compared to SnO2 nanoparticles, and Co3S4-SnO2 nanocomposites.
32544578	5	16	theme	Co3S4-SnO2	762:771	arg1	nanocomposites					773:786	Co3S4-SnO2 nanocomposites	762:786	Co3S4-SnO2 nanocomposites	762:786	The nano-structure Co3S4-SnO2/PVPCS composite (98.72%) revealed the highest photocatalysis performance as compared to SnO2 nanoparticles, and Co3S4-SnO2 nanocomposites.
32544578	5	17	theme	photocatalysis	696:709	arg1	performance					711:721	the highest photocatalysis performance	684:721	the highest photocatalysis performance	684:721	The nano-structure Co3S4-SnO2/PVPCS composite (98.72%) revealed the highest photocatalysis performance as compared to SnO2 nanoparticles, and Co3S4-SnO2 nanocomposites.
32544578	6	18	theme	composite	844:852	arg1	photo-stability					793:807	The photo-stability	789:807	The photo-stability of nano-structure Co3S4-SnO2/PVPCS composite	789:852	The photo-stability of nano-structure Co3S4-SnO2/PVPCS composite was characterized using cyclic catalytic experimental.
32544578	1	19	theme	PVPCS	259:263	arg1	nano-structure					266:279	Co3S4-SnO2 supported polyvinylpyrrolidone-cellulose (PVPCS) nano-structure	206:279	Co3S4-SnO2 supported polyvinylpyrrolidone-cellulose (PVPCS) nano-structure for Lidocaine degradation	206:305	In this work, we present Co3S4-SnO2 supported polyvinylpyrrolidone-cellulose (PVPCS) nano-structure for Lidocaine degradation.
32544578	8	20	dep	antibacterial	1101:1113	arg1	aureus					1138:1143	Staphylococcus aureus	1123:1143	Staphylococcus aureus	1123:1143	The biological properties of Co3S4-SnO2/PVPCS composite were investigated through the antibacterial (versus Staphylococcus aureus, and Escherichia coli) and antifungal studies (Candida albicans).
32544578	8	20	dep	antibacterial	1101:1113	arg1	coli					1162:1165	Escherichia coli	1150:1165	Escherichia coli	1150:1165	The biological properties of Co3S4-SnO2/PVPCS composite were investigated through the antibacterial (versus Staphylococcus aureus, and Escherichia coli) and antifungal studies (Candida albicans).
32544578	6	21	theme	Co3S4-SnO2/PVPCS	827:842	arg1	composite					844:852	nano-structure Co3S4-SnO2/PVPCS composite	812:852	nano-structure Co3S4-SnO2/PVPCS composite	812:852	The photo-stability of nano-structure Co3S4-SnO2/PVPCS composite was characterized using cyclic catalytic experimental.
32544578	0	22	theme	Co3S4-SnO2/polyvinylpyrrolidone-cellulose	13:53	arg1	Synthesis					0:8	Synthesis	0:8	Synthesis of Co3S4-SnO2/polyvinylpyrrolidone-cellulose	0:53	Synthesis of Co3S4-SnO2/polyvinylpyrrolidone-cellulose heterojunction as highly performance catalyst for photocatalytic and antimicrobial properties under ultra-violet irradiation.
32544578	7	23	theme	Co3S4-SnO2/PVPCS	987:1002	arg1	composite					1004:1012	the nano-structure Co3S4-SnO2/PVPCS composite	968:1012	the nano-structure Co3S4-SnO2/PVPCS composite	968:1012	Results demonstrated a substantially stable performance of the nano-structure Co3S4-SnO2/PVPCS composite.
32544578	2	24	theme	optical	389:395	arg1	ones					397:400	morphological and optical ones	371:400	morphological and optical ones	371:400	The nanostructure was characterized by various techniques i.e. morphological and optical ones.
32544578	6	25	theme	nano-structure	812:825	arg1	composite					844:852	nano-structure Co3S4-SnO2/PVPCS composite	812:852	nano-structure Co3S4-SnO2/PVPCS composite	812:852	The photo-stability of nano-structure Co3S4-SnO2/PVPCS composite was characterized using cyclic catalytic experimental.
32544578	7	26	theme	composite	1004:1012	arg1	performance					953:963	a substantially stable performance	930:963	a substantially stable performance of the nano-structure Co3S4-SnO2/PVPCS composite	930:1012	Results demonstrated a substantially stable performance of the nano-structure Co3S4-SnO2/PVPCS composite.
32544578	4	27	theme	catalysts	549:557	arg1	performances					529:540	the photocatalysis performances	510:540	the photocatalysis performances of the catalysts	510:557	Moreover, the photocatalysis performances of the catalysts were investigated under ultra-violet (UV) light irradiation.
32544578	8	28	theme	antifungal	1172:1181	arg1	studies					1183:1189	antifungal studies	1172:1189	antifungal studies (Candida albicans)	1172:1208	The biological properties of Co3S4-SnO2/PVPCS composite were investigated through the antibacterial (versus Staphylococcus aureus, and Escherichia coli) and antifungal studies (Candida albicans).
32544578	8	28	theme	antifungal	1172:1181	arg1	albicans					1200:1207	Candida albicans	1192:1207	Candida albicans	1192:1207	The biological properties of Co3S4-SnO2/PVPCS composite were investigated through the antibacterial (versus Staphylococcus aureus, and Escherichia coli) and antifungal studies (Candida albicans).
32544578	1	29	theme	Lidocaine	285:293	arg1	degradation					295:305	Lidocaine degradation	285:305	Lidocaine degradation	285:305	In this work, we present Co3S4-SnO2 supported polyvinylpyrrolidone-cellulose (PVPCS) nano-structure for Lidocaine degradation.
32544578	7	30	theme	nano-structure	972:985	arg1	composite					1004:1012	the nano-structure Co3S4-SnO2/PVPCS composite	968:1012	the nano-structure Co3S4-SnO2/PVPCS composite	968:1012	Results demonstrated a substantially stable performance of the nano-structure Co3S4-SnO2/PVPCS composite.
32544578	2	31	dep	ones	397:400	arg1	i.e.					366:369	i.e.	366:369	i.e.	366:369	The nanostructure was characterized by various techniques i.e. morphological and optical ones.
32544578	0	32	theme	performance	80:90	arg1	catalyst					92:99	highly performance catalyst	73:99	highly performance catalyst for photocatalytic and antimicrobial properties under ultra-violet irradiation	73:178	Synthesis of Co3S4-SnO2/polyvinylpyrrolidone-cellulose heterojunction as highly performance catalyst for photocatalytic and antimicrobial properties under ultra-violet irradiation.
32544578	9	33	theme	Co3S4-SnO2	1236:1245	arg1	nanocomposites					1247:1260	Co3S4-SnO2 nanocomposites	1236:1260	Co3S4-SnO2 nanocomposites	1236:1260	As the results declared, Co3S4-SnO2 nanocomposites have substantial biological properties as compared to SnO2 nanoparticles, and Co3S4-SnO2 nanocomposites.
32544578	4	34	theme	photocatalysis	514:527	arg1	performances					529:540	the photocatalysis performances	510:540	the photocatalysis performances of the catalysts	510:557	Moreover, the photocatalysis performances of the catalysts were investigated under ultra-violet (UV) light irradiation.
32544578	2	35	theme	various	347:353	arg1	techniques					355:364	various techniques	347:364	various techniques i.e. morphological and optical ones	347:400	The nanostructure was characterized by various techniques i.e. morphological and optical ones.
32544578	8	36	theme	biological	1019:1028	arg1	properties					1030:1039	The biological properties	1015:1039	The biological properties of Co3S4-SnO2/PVPCS composite	1015:1069	The biological properties of Co3S4-SnO2/PVPCS composite were investigated through the antibacterial (versus Staphylococcus aureus, and Escherichia coli) and antifungal studies (Candida albicans).
32544578	5	37	theme	nano-structure	624:637	arg1	composite					656:664	The nano-structure Co3S4-SnO2/PVPCS composite	620:664	The nano-structure Co3S4-SnO2/PVPCS composite (98.72%)	620:673	The nano-structure Co3S4-SnO2/PVPCS composite (98.72%) revealed the highest photocatalysis performance as compared to SnO2 nanoparticles, and Co3S4-SnO2 nanocomposites.
32544578	5	37	theme	nano-structure	624:637	arg1	%					672:672	98.72%	667:672	98.72%	667:672	The nano-structure Co3S4-SnO2/PVPCS composite (98.72%) revealed the highest photocatalysis performance as compared to SnO2 nanoparticles, and Co3S4-SnO2 nanocomposites.
32544578	9	38	theme	Co3S4-SnO2	1340:1349	arg1	nanocomposites					1351:1364	Co3S4-SnO2 nanocomposites	1340:1364	Co3S4-SnO2 nanocomposites	1340:1364	As the results declared, Co3S4-SnO2 nanocomposites have substantial biological properties as compared to SnO2 nanoparticles, and Co3S4-SnO2 nanocomposites.
32544578	8	39	theme	Co3S4-SnO2/PVPCS	1044:1059	arg1	composite					1061:1069	Co3S4-SnO2/PVPCS composite	1044:1069	Co3S4-SnO2/PVPCS composite	1044:1069	The biological properties of Co3S4-SnO2/PVPCS composite were investigated through the antibacterial (versus Staphylococcus aureus, and Escherichia coli) and antifungal studies (Candida albicans).
32544578	7	40	theme	stable	946:951	arg1	performance					953:963	a substantially stable performance	930:963	a substantially stable performance of the nano-structure Co3S4-SnO2/PVPCS composite	930:1012	Results demonstrated a substantially stable performance of the nano-structure Co3S4-SnO2/PVPCS composite.
32544578	0	41	theme	photocatalytic	105:118	arg1	properties					138:147	photocatalytic and antimicrobial properties	105:147	photocatalytic and antimicrobial properties under ultra-violet irradiation	105:178	Synthesis of Co3S4-SnO2/polyvinylpyrrolidone-cellulose heterojunction as highly performance catalyst for photocatalytic and antimicrobial properties under ultra-violet irradiation.
32544578	5	42	theme	Co3S4-SnO2/PVPCS	639:654	arg1	composite					656:664	The nano-structure Co3S4-SnO2/PVPCS composite	620:664	The nano-structure Co3S4-SnO2/PVPCS composite (98.72%)	620:673	The nano-structure Co3S4-SnO2/PVPCS composite (98.72%) revealed the highest photocatalysis performance as compared to SnO2 nanoparticles, and Co3S4-SnO2 nanocomposites.
32544578	5	42	theme	Co3S4-SnO2/PVPCS	639:654	arg1	%					672:672	98.72%	667:672	98.72%	667:672	The nano-structure Co3S4-SnO2/PVPCS composite (98.72%) revealed the highest photocatalysis performance as compared to SnO2 nanoparticles, and Co3S4-SnO2 nanocomposites.
32544578	9	43	theme	substantial	1267:1277	arg1	properties					1290:1299	substantial biological properties	1267:1299	substantial biological properties	1267:1299	As the results declared, Co3S4-SnO2 nanocomposites have substantial biological properties as compared to SnO2 nanoparticles, and Co3S4-SnO2 nanocomposites.
32544578	8	44	theme	Candida	1192:1198	arg1	studies					1183:1189	antifungal studies	1172:1189	antifungal studies (Candida albicans)	1172:1208	The biological properties of Co3S4-SnO2/PVPCS composite were investigated through the antibacterial (versus Staphylococcus aureus, and Escherichia coli) and antifungal studies (Candida albicans).
32544578	8	44	theme	Candida	1192:1198	arg1	albicans					1200:1207	Candida albicans	1192:1207	Candida albicans	1192:1207	The biological properties of Co3S4-SnO2/PVPCS composite were investigated through the antibacterial (versus Staphylococcus aureus, and Escherichia coli) and antifungal studies (Candida albicans).
32544578	9	45	contain	have	1262:1265	arg2	properties					1290:1299	substantial biological properties	1267:1299	substantial biological properties	1267:1299	As the results declared, Co3S4-SnO2 nanocomposites have substantial biological properties as compared to SnO2 nanoparticles, and Co3S4-SnO2 nanocomposites.
32544578	9	45	contain	have	1262:1265	arg1	nanocomposites					1247:1260	Co3S4-SnO2 nanocomposites	1236:1260	Co3S4-SnO2 nanocomposites	1236:1260	As the results declared, Co3S4-SnO2 nanocomposites have substantial biological properties as compared to SnO2 nanoparticles, and Co3S4-SnO2 nanocomposites.
33453383	0	0	theme	P450	163:166	arg1	antioxidant					65:75	antioxidant	65:75	antioxidant	65:75	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	0	0	theme	P450	163:166	arg1	modulation					127:136	transcriptomics modulation	111:136	transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein	111:189	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	0	0	theme	P450	163:166	arg1	growth					57:62	growth	57:62	growth	57:62	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	0	0	theme	P450	163:166	arg1	resistance					216:225	Aeromonas hydrophila resistance	195:225	Aeromonas hydrophila resistance	195:225	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	0	0	theme	P450	163:166	arg1	expression					99:108	immune related genes expression	78:108	immune related genes expression	78:108	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	17	1	theme	nano	2690:2693	arg1	composite					2695:2703	chitosan nano composite	2681:2703	chitosan nano composite	2681:2703	In conclusion, the ameliorative effects of SeChNPs on Nile tilapia growth resulted from immune stimulatory and free radicals scavenging effects of selenium loaded chitosan nano composite.
33453383	16	2	located	found	2396:2400	arg2	down-regulation					2332:2346	the maximum down-regulation	2320:2346	the maximum down-regulation of cytochromes P450 and heat shock protein	2320:2389	Moreover, the maximum down-regulation of cytochromes P450 and heat shock protein were found in 2 g/kg SeChNPs supplemented group (reduced by 0.4 and 0.6-fold, respectively, when compared with control group).
33453383	16	2	located	found	2396:2400	arg1	group					2433:2437	2 g/kg SeChNPs supplemented group	2405:2437	2 g/kg SeChNPs supplemented group (reduced by 0.4 and 0.6-fold, respectively, when compared with control group)	2405:2515	Moreover, the maximum down-regulation of cytochromes P450 and heat shock protein were found in 2 g/kg SeChNPs supplemented group (reduced by 0.4 and 0.6-fold, respectively, when compared with control group).
33453383	0	3	theme	heat	172:175	arg1	protein					183:189	heat shock protein	172:189	heat shock protein	172:189	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	17	4	from	effects	2550:2556	arg1	growth					2585:2590	Nile tilapia growth	2572:2590	Nile tilapia growth	2572:2590	In conclusion, the ameliorative effects of SeChNPs on Nile tilapia growth resulted from immune stimulatory and free radicals scavenging effects of selenium loaded chitosan nano composite.
33453383	0	5	theme	protein	183:189	arg1	antioxidant					65:75	antioxidant	65:75	antioxidant	65:75	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	0	5	theme	protein	183:189	arg1	modulation					127:136	transcriptomics modulation	111:136	transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein	111:189	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	0	5	theme	protein	183:189	arg1	growth					57:62	growth	57:62	growth	57:62	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	0	5	theme	protein	183:189	arg1	resistance					216:225	Aeromonas hydrophila resistance	195:225	Aeromonas hydrophila resistance	195:225	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	0	5	theme	protein	183:189	arg1	expression					99:108	immune related genes expression	78:108	immune related genes expression	78:108	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	0	6	dep	Aeromonas	195:203	arg1	hydrophila					205:214	hydrophila	205:214	hydrophila	205:214	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	10	7	theme	SeChNPs	1461:1467	arg1	inclusion					1448:1456	Dietary inclusion	1440:1456	Dietary inclusion of SeChNPs	1440:1467	Dietary inclusion of SeChNPs increased serum lysozyme, alternative complement and myeloperoxidase activities and immunoglobulin type M level.
33453383	0	8	theme	Aeromonas	195:203	arg1	resistance					216:225	Aeromonas hydrophila resistance	195:225	Aeromonas hydrophila resistance	195:225	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	3	9	theme	nanoparticles	552:564	arg1	impact					517:522	impact	517:522	impact of selenite-loaded chitosan nanoparticles (SeChNPs) on Nile tilapia	517:590	Till now, there is no available data on impact of selenite-loaded chitosan nanoparticles (SeChNPs) on Nile tilapia.
33453383	11	10	with	comparison	1759:1768	arg1	group					1783:1787	control group	1775:1787	control group	1775:1787	Supplementation of SeChNPs at the level of 2 g/kg up-regulated glutathione peroxidase, superoxide dismutase and catalase expression by 1.12, 4.9 and 2.31 folds respectively, in comparison with control group.
33453383	6	11	theme	2 g/kg	1071:1076	arg1	diet					1078:1081	SeChNPs 0.5, 1 and 2 g/kg diet	1052:1081	diet	1078:1081	The control group was fed a basal diet (with inorganic se), other groups fed diets supplemented with SeChNPs 0.5, 1 and 2 g/kg diet.
33453383	8	12	theme	final	1242:1246	arg1	gain					1253:1256	the highest final body gain	1230:1256	the highest final body gain	1230:1256	Fish groups fed SeChNPs (0.5 and 1 g/kg) exhibited the highest final body gain, better feed utilization.
33453383	13	13	theme	SeChNPs	1988:1994	arg1	levels					1978:1983	different levels	1968:1983	different levels of SeChNPs	1968:1994	The expression of IL-10, IL-8, TNF-α and IL-1β genes was up-regulated after dietary inclusion of different levels of SeChNPs in a dose dependent manner.
33453383	3	14	theme	selenite-loaded	527:541	arg1	SeChNPs					567:573	SeChNPs	567:573	SeChNPs	567:573	Till now, there is no available data on impact of selenite-loaded chitosan nanoparticles (SeChNPs) on Nile tilapia.
33453383	3	14	theme	selenite-loaded	527:541	arg1	nanoparticles					552:564	selenite-loaded chitosan nanoparticles	527:564	selenite-loaded chitosan nanoparticles (SeChNPs)	527:574	Till now, there is no available data on impact of selenite-loaded chitosan nanoparticles (SeChNPs) on Nile tilapia.
33453383	12	15	theme	reactive	1820:1827	arg1	protein					1829:1835	C- reactive protein	1817:1835	C- reactive protein	1817:1835	In contrast, the levels of C- reactive protein and malondialdehyde were reduced.
33453383	16	16	theme	2 g/kg	2405:2410	arg1	group					2433:2437	2 g/kg SeChNPs supplemented group	2405:2437	2 g/kg SeChNPs supplemented group (reduced by 0.4 and 0.6-fold, respectively, when compared with control group)	2405:2515	Moreover, the maximum down-regulation of cytochromes P450 and heat shock protein were found in 2 g/kg SeChNPs supplemented group (reduced by 0.4 and 0.6-fold, respectively, when compared with control group).
33453383	10	17	theme	complement	1507:1516	arg1	activities					1538:1547	serum lysozyme, alternative complement and myeloperoxidase activities	1479:1547	serum lysozyme, alternative complement and myeloperoxidase activities	1479:1547	Dietary inclusion of SeChNPs increased serum lysozyme, alternative complement and myeloperoxidase activities and immunoglobulin type M level.
33453383	16	18	theme	supplemented	2420:2431	arg1	group					2433:2437	2 g/kg SeChNPs supplemented group	2405:2437	2 g/kg SeChNPs supplemented group (reduced by 0.4 and 0.6-fold, respectively, when compared with control group)	2405:2515	Moreover, the maximum down-regulation of cytochromes P450 and heat shock protein were found in 2 g/kg SeChNPs supplemented group (reduced by 0.4 and 0.6-fold, respectively, when compared with control group).
33453383	6	19	with	diet	985:988	arg1	se					1006:1007	inorganic se	996:1007	inorganic se	996:1007	The control group was fed a basal diet (with inorganic se), other groups fed diets supplemented with SeChNPs 0.5, 1 and 2 g/kg diet.
33453383	10	20	theme	serum	1479:1483	arg1	lysozyme					1485:1492	serum lysozyme	1479:1492	serum lysozyme	1479:1492	Dietary inclusion of SeChNPs increased serum lysozyme, alternative complement and myeloperoxidase activities and immunoglobulin type M level.
33453383	0	21	theme	caspase	141:147	arg1	antioxidant					65:75	antioxidant	65:75	antioxidant	65:75	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	0	21	theme	caspase	141:147	arg1	modulation					127:136	transcriptomics modulation	111:136	transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein	111:189	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	0	21	theme	caspase	141:147	arg1	growth					57:62	growth	57:62	growth	57:62	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	0	21	theme	caspase	141:147	arg1	resistance					216:225	Aeromonas hydrophila resistance	195:225	Aeromonas hydrophila resistance	195:225	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	0	21	theme	caspase	141:147	arg1	expression					99:108	immune related genes expression	78:108	immune related genes expression	78:108	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	14	22	theme	survival	2169:2176	arg1	%					2186:2186	45%	2184:2186	45%	2184:2186	Post-challenge, the highest survival rate was detected in group fed 2 g/kg SeChNPs (93%) in contrast, the control group was displayed the lowest survival rate (45%).
33453383	14	22	theme	survival	2169:2176	arg1	rate					2178:2181	the lowest survival rate	2158:2181	the lowest survival rate (45%)	2158:2187	Post-challenge, the highest survival rate was detected in group fed 2 g/kg SeChNPs (93%) in contrast, the control group was displayed the lowest survival rate (45%).
33453383	13	23	theme	levels	1978:1983	arg1	inclusion					1955:1963	dietary inclusion	1947:1963	dietary inclusion of different levels of SeChNPs	1947:1994	The expression of IL-10, IL-8, TNF-α and IL-1β genes was up-regulated after dietary inclusion of different levels of SeChNPs in a dose dependent manner.
33453383	6	24	theme	basal	979:983	arg1	groups					1017:1022	other groups	1011:1022	other groups fed diets supplemented with SeChNPs 0.5, 1 and 2 g/kg diet	1011:1081	The control group was fed a basal diet (with inorganic se), other groups fed diets supplemented with SeChNPs 0.5, 1 and 2 g/kg diet.
33453383	6	24	theme	basal	979:983	arg1	diet					985:988	a basal diet	977:988	a basal diet (with inorganic se)	977:1008	The control group was fed a basal diet (with inorganic se), other groups fed diets supplemented with SeChNPs 0.5, 1 and 2 g/kg diet.
33453383	17	25	theme	tilapia	2577:2583	arg1	growth					2585:2590	Nile tilapia growth	2572:2590	Nile tilapia growth	2572:2590	In conclusion, the ameliorative effects of SeChNPs on Nile tilapia growth resulted from immune stimulatory and free radicals scavenging effects of selenium loaded chitosan nano composite.
33453383	4	26	theme	chitosan	662:669	arg1	supplementation					685:699	selenite-loaded chitosan nanoparticles supplementation	646:699	selenite-loaded chitosan nanoparticles supplementation	646:699	Hence, the current study investigated the effects of selenite-loaded chitosan nanoparticles supplementation on the growth, immune, antioxidant and apoptotic related genes as well as resistance to Aeromonas hydrophila of Nile tilapia, Oreochromis niloticus.
33453383	15	27	theme	caspase	2244:2250	arg1	expression					2230:2239	the expression	2226:2239	the expression of caspase 1	2226:2252	After challenge with A. hydrophila, the expression of caspase 1 was up-regulated in groups fed 1 and 2 g/kg of SeChNPs.
33453383	5	28	theme	fish	865:868	arg1	total					852:856	A total	850:856	A total of 400 fish	850:868	A total of 400 fish were randomly divided into four groups, and each group retained five replicates.
33453383	4	29	theme	supplementation	685:699	arg1	effects					635:641	the effects	631:641	the effects of selenite-loaded chitosan nanoparticles supplementation on the growth, immune, antioxidant and apoptotic related genes as well as resistance to Aeromonas hydrophila of Nile tilapia, Oreochromis niloticus	631:847	Hence, the current study investigated the effects of selenite-loaded chitosan nanoparticles supplementation on the growth, immune, antioxidant and apoptotic related genes as well as resistance to Aeromonas hydrophila of Nile tilapia, Oreochromis niloticus.
33453383	3	30	from	data	509:512	arg1	impact					517:522	impact	517:522	impact of selenite-loaded chitosan nanoparticles (SeChNPs) on Nile tilapia	517:590	Till now, there is no available data on impact of selenite-loaded chitosan nanoparticles (SeChNPs) on Nile tilapia.
33453383	11	31	theme	superoxide	1669:1678	arg1	dismutase					1680:1688	superoxide dismutase	1669:1688	superoxide dismutase	1669:1688	Supplementation of SeChNPs at the level of 2 g/kg up-regulated glutathione peroxidase, superoxide dismutase and catalase expression by 1.12, 4.9 and 2.31 folds respectively, in comparison with control group.
33453383	1	32	theme	aquaculture	326:336	arg1	production					338:347	aquaculture production	326:347	aquaculture production	326:347	Nowadays there is a great attention for nanotechnology in aquaculture production.
33453383	16	33	theme	heat	2372:2375	arg1	protein					2383:2389	heat shock protein	2372:2389	heat shock protein	2372:2389	Moreover, the maximum down-regulation of cytochromes P450 and heat shock protein were found in 2 g/kg SeChNPs supplemented group (reduced by 0.4 and 0.6-fold, respectively, when compared with control group).
33453383	16	33	theme	heat	2372:2375	arg1	cytochromes					2351:2361	cytochromes P450 and heat shock protein	2351:2389	cytochromes P450 and heat shock protein	2351:2389	Moreover, the maximum down-regulation of cytochromes P450 and heat shock protein were found in 2 g/kg SeChNPs supplemented group (reduced by 0.4 and 0.6-fold, respectively, when compared with control group).
33453383	1	34	dep	Nowadays	268:275	arg1	there					277:281	there	277:281	there	277:281	Nowadays there is a great attention for nanotechnology in aquaculture production.
33453383	4	35	theme	related	750:756	arg1	growth					708:713	the growth	704:713	the growth	704:713	Hence, the current study investigated the effects of selenite-loaded chitosan nanoparticles supplementation on the growth, immune, antioxidant and apoptotic related genes as well as resistance to Aeromonas hydrophila of Nile tilapia, Oreochromis niloticus.
33453383	4	35	theme	related	750:756	arg1	genes					758:762	related genes	750:762	related genes	750:762	Hence, the current study investigated the effects of selenite-loaded chitosan nanoparticles supplementation on the growth, immune, antioxidant and apoptotic related genes as well as resistance to Aeromonas hydrophila of Nile tilapia, Oreochromis niloticus.
33453383	3	36	from	impact	517:522	arg1	tilapia					584:590	Nile tilapia	579:590	Nile tilapia	579:590	Till now, there is no available data on impact of selenite-loaded chitosan nanoparticles (SeChNPs) on Nile tilapia.
33453383	11	37	theme	peroxidase	1657:1666	arg1	expression					1703:1712	glutathione peroxidase, superoxide dismutase and catalase expression	1645:1712	glutathione peroxidase, superoxide dismutase and catalase expression	1645:1712	Supplementation of SeChNPs at the level of 2 g/kg up-regulated glutathione peroxidase, superoxide dismutase and catalase expression by 1.12, 4.9 and 2.31 folds respectively, in comparison with control group.
33453383	0	38	theme	immune	78:83	arg1	genes					93:97	immune related genes	78:97	immune related genes expression	78:108	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	11	39	dep	1.12	1717:1720	arg1	folds					1736:1740	folds	1736:1740	folds respectively	1736:1753	Supplementation of SeChNPs at the level of 2 g/kg up-regulated glutathione peroxidase, superoxide dismutase and catalase expression by 1.12, 4.9 and 2.31 folds respectively, in comparison with control group.
33453383	0	40	theme	Tilapia	235:241	arg1	antioxidant					65:75	antioxidant	65:75	antioxidant	65:75	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	0	40	theme	Tilapia	235:241	arg1	modulation					127:136	transcriptomics modulation	111:136	transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein	111:189	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	0	40	theme	Tilapia	235:241	arg1	growth					57:62	growth	57:62	growth	57:62	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	0	40	theme	Tilapia	235:241	arg1	resistance					216:225	Aeromonas hydrophila resistance	195:225	Aeromonas hydrophila resistance	195:225	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	0	40	theme	Tilapia	235:241	arg1	expression					99:108	immune related genes expression	78:108	immune related genes expression	78:108	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	11	41	from	Supplementation	1582:1596	arg1	level					1616:1620	the level	1612:1620	the level of 2 g/kg	1612:1630	Supplementation of SeChNPs at the level of 2 g/kg up-regulated glutathione peroxidase, superoxide dismutase and catalase expression by 1.12, 4.9 and 2.31 folds respectively, in comparison with control group.
33453383	0	42	from	nanoparticles	40:52	arg1	modulation					127:136	transcriptomics modulation	111:136	transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein	111:189	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	0	42	from	nanoparticles	40:52	arg1	antioxidant					65:75	antioxidant	65:75	antioxidant	65:75	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	0	42	from	nanoparticles	40:52	arg1	resistance					216:225	Aeromonas hydrophila resistance	195:225	Aeromonas hydrophila resistance	195:225	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	0	42	from	nanoparticles	40:52	arg1	growth					57:62	growth	57:62	growth	57:62	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	0	42	from	nanoparticles	40:52	arg1	expression					99:108	immune related genes expression	78:108	immune related genes expression	78:108	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	8	43	theme	highest	1234:1240	arg1	gain					1253:1256	the highest final body gain	1230:1256	the highest final body gain	1230:1256	Fish groups fed SeChNPs (0.5 and 1 g/kg) exhibited the highest final body gain, better feed utilization.
33453383	0	44	theme	genes	93:97	arg1	expression					99:108	immune related genes expression	78:108	immune related genes expression	78:108	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	2	45	contain	has	353:355	arg1	It					350:351	It	350:351	It	350:351	It has an efficient role in nutrients and drugs delivery, ponds sterilization, water treatment and aquatic diseases reduction.
33453383	2	45	contain	has	353:355	arg2	role					370:373	an efficient role	357:373	an efficient role in nutrients and drugs delivery, ponds sterilization, water treatment and aquatic diseases reduction	357:474	It has an efficient role in nutrients and drugs delivery, ponds sterilization, water treatment and aquatic diseases reduction.
33453383	0	46	theme	Oreochromis	244:254	arg1	Tilapia					235:241	Nile Tilapia	230:241	Nile Tilapia (Oreochromis niloticus)	230:265	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	0	46	theme	Oreochromis	244:254	arg1	niloticus					256:264	Oreochromis niloticus	244:264	Oreochromis niloticus	244:264	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	6	47	theme	other	1011:1015	arg1	diet					985:988	a basal diet	977:988	a basal diet (with inorganic se)	977:1008	The control group was fed a basal diet (with inorganic se), other groups fed diets supplemented with SeChNPs 0.5, 1 and 2 g/kg diet.
33453383	6	47	theme	other	1011:1015	arg1	groups					1017:1022	other groups	1011:1022	other groups fed diets supplemented with SeChNPs 0.5, 1 and 2 g/kg diet	1011:1081	The control group was fed a basal diet (with inorganic se), other groups fed diets supplemented with SeChNPs 0.5, 1 and 2 g/kg diet.
33453383	2	48	theme	water	429:433	arg1	treatment					435:443	water treatment	429:443	water treatment	429:443	It has an efficient role in nutrients and drugs delivery, ponds sterilization, water treatment and aquatic diseases reduction.
33453383	8	49	theme	body	1248:1251	arg1	gain					1253:1256	the highest final body gain	1230:1256	the highest final body gain	1230:1256	Fish groups fed SeChNPs (0.5 and 1 g/kg) exhibited the highest final body gain, better feed utilization.
33453383	0	50	theme	Dual	0:3	arg1	effect					5:10	Dual effect	0:10	Dual effect of selenium	0:22	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	16	51	theme	control	2502:2508	arg1	group					2510:2514	control group	2502:2514	control group	2502:2514	Moreover, the maximum down-regulation of cytochromes P450 and heat shock protein were found in 2 g/kg SeChNPs supplemented group (reduced by 0.4 and 0.6-fold, respectively, when compared with control group).
33453383	17	52	theme	ameliorative	2537:2548	arg1	effects					2550:2556	the ameliorative effects	2533:2556	the ameliorative effects of SeChNPs on Nile tilapia growth	2533:2590	In conclusion, the ameliorative effects of SeChNPs on Nile tilapia growth resulted from immune stimulatory and free radicals scavenging effects of selenium loaded chitosan nano composite.
33453383	10	53	theme	M	1573:1573	arg1	level					1575:1579	immunoglobulin type M level	1553:1579	immunoglobulin type M level	1553:1579	Dietary inclusion of SeChNPs increased serum lysozyme, alternative complement and myeloperoxidase activities and immunoglobulin type M level.
33453383	7	54	theme	loading	1088:1094	arg1	concentration					1096:1108	The loading concentration	1084:1108	The loading concentration of Se to ChNPs	1084:1123	The loading concentration of Se to ChNPs was 0.3, 0.6 and 1.2 mg/0.5, 1 and 2 gm respectively.
33453383	7	54	theme	loading	1088:1094	arg1	mg/0.5					1146:1151	mg/0.5	1146:1151	mg/0.5	1146:1151	The loading concentration of Se to ChNPs was 0.3, 0.6 and 1.2 mg/0.5, 1 and 2 gm respectively.
33453383	16	55	dep	group	2433:2437	arg1	reduced					2440:2446	reduced	2440:2446	reduced	2440:2446	Moreover, the maximum down-regulation of cytochromes P450 and heat shock protein were found in 2 g/kg SeChNPs supplemented group (reduced by 0.4 and 0.6-fold, respectively, when compared with control group).
33453383	14	56	dep	SeChNPs	2099:2105	arg1	displayed					2148:2156	displayed	2148:2156	was displayed the lowest survival rate (45%)	2144:2187	Post-challenge, the highest survival rate was detected in group fed 2 g/kg SeChNPs (93%) in contrast, the control group was displayed the lowest survival rate (45%).
33453383	6	57	theme	inorganic	996:1004	arg1	se					1006:1007	inorganic se	996:1007	inorganic se	996:1007	The control group was fed a basal diet (with inorganic se), other groups fed diets supplemented with SeChNPs 0.5, 1 and 2 g/kg diet.
33453383	8	58	theme	feed	1266:1269	arg1	utilization					1271:1281	better feed utilization	1259:1281	better feed utilization	1259:1281	Fish groups fed SeChNPs (0.5 and 1 g/kg) exhibited the highest final body gain, better feed utilization.
33453383	10	59	theme	immunoglobulin	1553:1566	arg1	level					1575:1579	immunoglobulin type M level	1553:1579	immunoglobulin type M level	1553:1579	Dietary inclusion of SeChNPs increased serum lysozyme, alternative complement and myeloperoxidase activities and immunoglobulin type M level.
33453383	17	60	theme	SeChNPs	2561:2567	arg1	effects					2550:2556	the ameliorative effects	2533:2556	the ameliorative effects of SeChNPs on Nile tilapia growth	2533:2590	In conclusion, the ameliorative effects of SeChNPs on Nile tilapia growth resulted from immune stimulatory and free radicals scavenging effects of selenium loaded chitosan nano composite.
33453383	2	61	from	role	370:373	arg1	drugs					392:396	drugs	392:396	drugs	392:396	It has an efficient role in nutrients and drugs delivery, ponds sterilization, water treatment and aquatic diseases reduction.
33453383	2	61	from	role	370:373	arg1	nutrients					378:386	nutrients	378:386	nutrients	378:386	It has an efficient role in nutrients and drugs delivery, ponds sterilization, water treatment and aquatic diseases reduction.
33453383	4	62	theme	Nile	813:816	arg1	tilapia					818:824	Nile tilapia	813:824	Nile tilapia	813:824	Hence, the current study investigated the effects of selenite-loaded chitosan nanoparticles supplementation on the growth, immune, antioxidant and apoptotic related genes as well as resistance to Aeromonas hydrophila of Nile tilapia, Oreochromis niloticus.
33453383	13	63	theme	IL-8	1896:1899	arg1	genes					1918:1922	IL-10, IL-8, TNF-α and IL-1β genes	1889:1922	IL-10, IL-8, TNF-α and IL-1β genes	1889:1922	The expression of IL-10, IL-8, TNF-α and IL-1β genes was up-regulated after dietary inclusion of different levels of SeChNPs in a dose dependent manner.
33453383	3	64	theme	Till	477:480	arg1	now					482:484	Till now	477:484	Till now	477:484	Till now, there is no available data on impact of selenite-loaded chitosan nanoparticles (SeChNPs) on Nile tilapia.
33453383	2	65	dep	role	370:373	arg1	delivery					398:405	delivery	398:405	delivery	398:405	It has an efficient role in nutrients and drugs delivery, ponds sterilization, water treatment and aquatic diseases reduction.
33453383	2	65	dep	role	370:373	arg1	sterilization					414:426	ponds sterilization	408:426	ponds sterilization	408:426	It has an efficient role in nutrients and drugs delivery, ponds sterilization, water treatment and aquatic diseases reduction.
33453383	2	65	dep	role	370:373	arg1	treatment					435:443	water treatment	429:443	water treatment	429:443	It has an efficient role in nutrients and drugs delivery, ponds sterilization, water treatment and aquatic diseases reduction.
33453383	2	65	dep	role	370:373	arg1	diseases					457:464	aquatic diseases	449:464	aquatic diseases	449:464	It has an efficient role in nutrients and drugs delivery, ponds sterilization, water treatment and aquatic diseases reduction.
33453383	13	66	theme	TNF-α	1902:1906	arg1	genes					1918:1922	IL-10, IL-8, TNF-α and IL-1β genes	1889:1922	IL-10, IL-8, TNF-α and IL-1β genes	1889:1922	The expression of IL-10, IL-8, TNF-α and IL-1β genes was up-regulated after dietary inclusion of different levels of SeChNPs in a dose dependent manner.
33453383	8	67	dep	fed	1191:1193	arg1	exhibited					1220:1228	exhibited	1220:1228	exhibited the highest final body gain	1220:1256	Fish groups fed SeChNPs (0.5 and 1 g/kg) exhibited the highest final body gain, better feed utilization.
33453383	6	68	theme	SeChNPs	1052:1058	arg1	diet					1078:1081	SeChNPs 0.5, 1 and 2 g/kg diet	1052:1081	diet	1078:1081	The control group was fed a basal diet (with inorganic se), other groups fed diets supplemented with SeChNPs 0.5, 1 and 2 g/kg diet.
33453383	17	69	theme	selenium	2665:2672	arg1	effects					2654:2660	immune stimulatory and free radicals scavenging effects	2606:2660	immune stimulatory and free radicals scavenging effects of selenium loaded chitosan nano composite	2606:2703	In conclusion, the ameliorative effects of SeChNPs on Nile tilapia growth resulted from immune stimulatory and free radicals scavenging effects of selenium loaded chitosan nano composite.
33453383	9	70	theme	myostatin	1316:1324	arg1	gene					1326:1329	myostatin gene	1316:1329	myostatin gene	1316:1329	Additionally, the expression of myostatin gene was down-regulated by 0.2 and 0.3 fold in group fed 0.5 and 1 g/kg SeChNPs when compared with control group.
33453383	1	71	from	attention	294:302	arg1	production					338:347	aquaculture production	326:347	aquaculture production	326:347	Nowadays there is a great attention for nanotechnology in aquaculture production.
33453383	13	72	theme	IL-10	1889:1893	arg1	genes					1918:1922	IL-10, IL-8, TNF-α and IL-1β genes	1889:1922	IL-10, IL-8, TNF-α and IL-1β genes	1889:1922	The expression of IL-10, IL-8, TNF-α and IL-1β genes was up-regulated after dietary inclusion of different levels of SeChNPs in a dose dependent manner.
33453383	17	73	theme	chitosan	2681:2688	arg1	composite					2695:2703	chitosan nano composite	2681:2703	chitosan nano composite	2681:2703	In conclusion, the ameliorative effects of SeChNPs on Nile tilapia growth resulted from immune stimulatory and free radicals scavenging effects of selenium loaded chitosan nano composite.
33453383	13	74	theme	dependent	2006:2014	arg1	manner					2016:2021	a dose dependent manner	1999:2021	a dose dependent manner	1999:2021	The expression of IL-10, IL-8, TNF-α and IL-1β genes was up-regulated after dietary inclusion of different levels of SeChNPs in a dose dependent manner.
33453383	10	75	theme	Dietary	1440:1446	arg1	inclusion					1448:1456	Dietary inclusion	1440:1456	Dietary inclusion of SeChNPs	1440:1467	Dietary inclusion of SeChNPs increased serum lysozyme, alternative complement and myeloperoxidase activities and immunoglobulin type M level.
33453383	0	76	theme	shock	177:181	arg1	protein					183:189	heat shock protein	172:189	heat shock protein	172:189	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	16	77	dep	cytochromes	2351:2361	arg1	P450					2363:2366	P450	2363:2366	P450	2363:2366	Moreover, the maximum down-regulation of cytochromes P450 and heat shock protein were found in 2 g/kg SeChNPs supplemented group (reduced by 0.4 and 0.6-fold, respectively, when compared with control group).
33453383	16	77	dep	cytochromes	2351:2361	arg1	protein					2383:2389	heat shock protein	2372:2389	heat shock protein	2372:2389	Moreover, the maximum down-regulation of cytochromes P450 and heat shock protein were found in 2 g/kg SeChNPs supplemented group (reduced by 0.4 and 0.6-fold, respectively, when compared with control group).
33453383	16	77	dep	cytochromes	2351:2361	arg1	cytochromes					2351:2361	cytochromes P450 and heat shock protein	2351:2389	cytochromes P450 and heat shock protein	2351:2389	Moreover, the maximum down-regulation of cytochromes P450 and heat shock protein were found in 2 g/kg SeChNPs supplemented group (reduced by 0.4 and 0.6-fold, respectively, when compared with control group).
33453383	14	78	located	detected	2070:2077	arg2	rate					2061:2064	Post-challenge, the highest survival rate	2024:2064	rate	2061:2064	Post-challenge, the highest survival rate was detected in group fed 2 g/kg SeChNPs (93%) in contrast, the control group was displayed the lowest survival rate (45%).
33453383	14	78	located	detected	2070:2077	arg1	group					2082:2086	group	2082:2086	group fed 2 g/kg SeChNPs (93%) in contrast, the control group was displayed the lowest survival rate (45%)	2082:2187	Post-challenge, the highest survival rate was detected in group fed 2 g/kg SeChNPs (93%) in contrast, the control group was displayed the lowest survival rate (45%).
33453383	17	79	theme	radicals	2634:2641	arg1	effects					2654:2660	immune stimulatory and free radicals scavenging effects	2606:2660	immune stimulatory and free radicals scavenging effects of selenium loaded chitosan nano composite	2606:2703	In conclusion, the ameliorative effects of SeChNPs on Nile tilapia growth resulted from immune stimulatory and free radicals scavenging effects of selenium loaded chitosan nano composite.
33453383	3	80	theme	available	499:507	arg1	data					509:512	no available data	496:512	no available data on impact of selenite-loaded chitosan nanoparticles (SeChNPs) on Nile tilapia	496:590	Till now, there is no available data on impact of selenite-loaded chitosan nanoparticles (SeChNPs) on Nile tilapia.
33453383	4	81	from	effects	635:641	arg1	resistance					775:784	resistance	775:784	resistance to Aeromonas hydrophila of Nile tilapia	775:824	Hence, the current study investigated the effects of selenite-loaded chitosan nanoparticles supplementation on the growth, immune, antioxidant and apoptotic related genes as well as resistance to Aeromonas hydrophila of Nile tilapia, Oreochromis niloticus.
33453383	4	81	from	effects	635:641	arg1	genes					758:762	related genes	750:762	related genes	750:762	Hence, the current study investigated the effects of selenite-loaded chitosan nanoparticles supplementation on the growth, immune, antioxidant and apoptotic related genes as well as resistance to Aeromonas hydrophila of Nile tilapia, Oreochromis niloticus.
33453383	4	81	from	effects	635:641	arg1	niloticus					839:847	Oreochromis niloticus	827:847	Oreochromis niloticus	827:847	Hence, the current study investigated the effects of selenite-loaded chitosan nanoparticles supplementation on the growth, immune, antioxidant and apoptotic related genes as well as resistance to Aeromonas hydrophila of Nile tilapia, Oreochromis niloticus.
33453383	4	81	from	effects	635:641	arg1	growth					708:713	the growth	704:713	the growth	704:713	Hence, the current study investigated the effects of selenite-loaded chitosan nanoparticles supplementation on the growth, immune, antioxidant and apoptotic related genes as well as resistance to Aeromonas hydrophila of Nile tilapia, Oreochromis niloticus.
33453383	14	82	theme	highest	2044:2050	arg1	rate					2061:2064	Post-challenge, the highest survival rate	2024:2064	rate	2061:2064	Post-challenge, the highest survival rate was detected in group fed 2 g/kg SeChNPs (93%) in contrast, the control group was displayed the lowest survival rate (45%).
33453383	14	83	theme	survival	2052:2059	arg1	rate					2061:2064	Post-challenge, the highest survival rate	2024:2064	rate	2061:2064	Post-challenge, the highest survival rate was detected in group fed 2 g/kg SeChNPs (93%) in contrast, the control group was displayed the lowest survival rate (45%).
33453383	3	84	theme	chitosan	543:550	arg1	SeChNPs					567:573	SeChNPs	567:573	SeChNPs	567:573	Till now, there is no available data on impact of selenite-loaded chitosan nanoparticles (SeChNPs) on Nile tilapia.
33453383	3	84	theme	chitosan	543:550	arg1	nanoparticles					552:564	selenite-loaded chitosan nanoparticles	527:564	selenite-loaded chitosan nanoparticles (SeChNPs)	527:574	Till now, there is no available data on impact of selenite-loaded chitosan nanoparticles (SeChNPs) on Nile tilapia.
33453383	9	85	theme	gene	1326:1329	arg1	expression					1302:1311	the expression	1298:1311	the expression of myostatin gene	1298:1329	Additionally, the expression of myostatin gene was down-regulated by 0.2 and 0.3 fold in group fed 0.5 and 1 g/kg SeChNPs when compared with control group.
33453383	12	86	theme	malondialdehyde	1841:1855	arg1	levels					1807:1812	the levels	1803:1812	the levels of C- reactive protein and malondialdehyde	1803:1855	In contrast, the levels of C- reactive protein and malondialdehyde were reduced.
33453383	4	87	theme	Oreochromis	827:837	arg1	niloticus					839:847	Oreochromis niloticus	827:847	Oreochromis niloticus	827:847	Hence, the current study investigated the effects of selenite-loaded chitosan nanoparticles supplementation on the growth, immune, antioxidant and apoptotic related genes as well as resistance to Aeromonas hydrophila of Nile tilapia, Oreochromis niloticus.
33453383	4	87	theme	Oreochromis	827:837	arg1	growth					708:713	the growth	704:713	the growth	704:713	Hence, the current study investigated the effects of selenite-loaded chitosan nanoparticles supplementation on the growth, immune, antioxidant and apoptotic related genes as well as resistance to Aeromonas hydrophila of Nile tilapia, Oreochromis niloticus.
33453383	0	88	theme	transcriptomics	111:125	arg1	modulation					127:136	transcriptomics modulation	111:136	transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein	111:189	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	16	89	theme	SeChNPs	2412:2418	arg1	group					2433:2437	2 g/kg SeChNPs supplemented group	2405:2437	2 g/kg SeChNPs supplemented group (reduced by 0.4 and 0.6-fold, respectively, when compared with control group)	2405:2515	Moreover, the maximum down-regulation of cytochromes P450 and heat shock protein were found in 2 g/kg SeChNPs supplemented group (reduced by 0.4 and 0.6-fold, respectively, when compared with control group).
33453383	10	90	theme	alternative	1495:1505	arg1	complement					1507:1516	alternative complement	1495:1516	alternative complement	1495:1516	Dietary inclusion of SeChNPs increased serum lysozyme, alternative complement and myeloperoxidase activities and immunoglobulin type M level.
33453383	11	91	theme	catalase	1694:1701	arg1	expression					1703:1712	glutathione peroxidase, superoxide dismutase and catalase expression	1645:1712	glutathione peroxidase, superoxide dismutase and catalase expression	1645:1712	Supplementation of SeChNPs at the level of 2 g/kg up-regulated glutathione peroxidase, superoxide dismutase and catalase expression by 1.12, 4.9 and 2.31 folds respectively, in comparison with control group.
33453383	12	92	theme	C-	1817:1818	arg1	protein					1829:1835	C- reactive protein	1817:1835	C- reactive protein	1817:1835	In contrast, the levels of C- reactive protein and malondialdehyde were reduced.
33453383	4	93	theme	current	604:610	arg1	study					612:616	the current study	600:616	the current study	600:616	Hence, the current study investigated the effects of selenite-loaded chitosan nanoparticles supplementation on the growth, immune, antioxidant and apoptotic related genes as well as resistance to Aeromonas hydrophila of Nile tilapia, Oreochromis niloticus.
33453383	10	94	theme	lysozyme	1485:1492	arg1	activities					1538:1547	serum lysozyme, alternative complement and myeloperoxidase activities	1479:1547	serum lysozyme, alternative complement and myeloperoxidase activities	1479:1547	Dietary inclusion of SeChNPs increased serum lysozyme, alternative complement and myeloperoxidase activities and immunoglobulin type M level.
33453383	12	95	theme	protein	1829:1835	arg1	levels					1807:1812	the levels	1803:1812	the levels of C- reactive protein and malondialdehyde	1803:1855	In contrast, the levels of C- reactive protein and malondialdehyde were reduced.
33453383	8	96	theme	Fish	1179:1182	arg1	groups					1184:1189	Fish groups	1179:1189	Fish groups	1179:1189	Fish groups fed SeChNPs (0.5 and 1 g/kg) exhibited the highest final body gain, better feed utilization.
33453383	0	97	theme	cytochrome	152:161	arg1	P450					163:166	cytochrome P450	152:166	cytochrome P450	152:166	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	13	98	theme	different	1968:1976	arg1	levels					1978:1983	different levels	1968:1983	different levels of SeChNPs	1968:1994	The expression of IL-10, IL-8, TNF-α and IL-1β genes was up-regulated after dietary inclusion of different levels of SeChNPs in a dose dependent manner.
33453383	16	99	theme	cytochromes	2351:2361	arg1	down-regulation					2332:2346	the maximum down-regulation	2320:2346	the maximum down-regulation of cytochromes P450 and heat shock protein	2320:2389	Moreover, the maximum down-regulation of cytochromes P450 and heat shock protein were found in 2 g/kg SeChNPs supplemented group (reduced by 0.4 and 0.6-fold, respectively, when compared with control group).
33453383	5	100	dep	five	934:937	arg1	replicates					939:948	replicates	939:948	replicates	939:948	A total of 400 fish were randomly divided into four groups, and each group retained five replicates.
33453383	14	101	theme	lowest	2162:2167	arg1	%					2186:2186	45%	2184:2186	45%	2184:2186	Post-challenge, the highest survival rate was detected in group fed 2 g/kg SeChNPs (93%) in contrast, the control group was displayed the lowest survival rate (45%).
33453383	14	101	theme	lowest	2162:2167	arg1	rate					2178:2181	the lowest survival rate	2158:2181	the lowest survival rate (45%)	2158:2187	Post-challenge, the highest survival rate was detected in group fed 2 g/kg SeChNPs (93%) in contrast, the control group was displayed the lowest survival rate (45%).
33453383	11	102	theme	glutathione	1645:1655	arg1	peroxidase					1657:1666	glutathione peroxidase	1645:1666	glutathione peroxidase	1645:1666	Supplementation of SeChNPs at the level of 2 g/kg up-regulated glutathione peroxidase, superoxide dismutase and catalase expression by 1.12, 4.9 and 2.31 folds respectively, in comparison with control group.
33453383	14	103	theme	control	2130:2136	arg1	group					2138:2142	the control group	2126:2142	the control group	2126:2142	Post-challenge, the highest survival rate was detected in group fed 2 g/kg SeChNPs (93%) in contrast, the control group was displayed the lowest survival rate (45%).
33453383	3	104	theme	Nile	579:582	arg1	tilapia					584:590	Nile tilapia	579:590	Nile tilapia	579:590	Till now, there is no available data on impact of selenite-loaded chitosan nanoparticles (SeChNPs) on Nile tilapia.
33453383	4	105	theme	selenite-loaded	646:660	arg1	supplementation					685:699	selenite-loaded chitosan nanoparticles supplementation	646:699	selenite-loaded chitosan nanoparticles supplementation	646:699	Hence, the current study investigated the effects of selenite-loaded chitosan nanoparticles supplementation on the growth, immune, antioxidant and apoptotic related genes as well as resistance to Aeromonas hydrophila of Nile tilapia, Oreochromis niloticus.
33453383	11	106	theme	2 g/kg	1625:1630	arg1	level					1616:1620	the level	1612:1620	the level of 2 g/kg	1612:1630	Supplementation of SeChNPs at the level of 2 g/kg up-regulated glutathione peroxidase, superoxide dismutase and catalase expression by 1.12, 4.9 and 2.31 folds respectively, in comparison with control group.
33453383	16	107	theme	shock	2377:2381	arg1	protein					2383:2389	heat shock protein	2372:2389	heat shock protein	2372:2389	Moreover, the maximum down-regulation of cytochromes P450 and heat shock protein were found in 2 g/kg SeChNPs supplemented group (reduced by 0.4 and 0.6-fold, respectively, when compared with control group).
33453383	16	107	theme	shock	2377:2381	arg1	cytochromes					2351:2361	cytochromes P450 and heat shock protein	2351:2389	cytochromes P450 and heat shock protein	2351:2389	Moreover, the maximum down-regulation of cytochromes P450 and heat shock protein were found in 2 g/kg SeChNPs supplemented group (reduced by 0.4 and 0.6-fold, respectively, when compared with control group).
33453383	11	108	from	level	1616:1620	arg1	Supplementation					1582:1596	Supplementation	1582:1596	Supplementation of SeChNPs at the level of 2 g/kg	1582:1630	Supplementation of SeChNPs at the level of 2 g/kg up-regulated glutathione peroxidase, superoxide dismutase and catalase expression by 1.12, 4.9 and 2.31 folds respectively, in comparison with control group.
33453383	11	108	from	level	1616:1620	arg1	SeChNPs					1601:1607	SeChNPs	1601:1607	SeChNPs at the level of 2 g/kg	1601:1630	Supplementation of SeChNPs at the level of 2 g/kg up-regulated glutathione peroxidase, superoxide dismutase and catalase expression by 1.12, 4.9 and 2.31 folds respectively, in comparison with control group.
33453383	4	109	theme	nanoparticles	671:683	arg1	supplementation					685:699	selenite-loaded chitosan nanoparticles supplementation	646:699	selenite-loaded chitosan nanoparticles supplementation	646:699	Hence, the current study investigated the effects of selenite-loaded chitosan nanoparticles supplementation on the growth, immune, antioxidant and apoptotic related genes as well as resistance to Aeromonas hydrophila of Nile tilapia, Oreochromis niloticus.
33453383	11	110	theme	dismutase	1680:1688	arg1	expression					1703:1712	glutathione peroxidase, superoxide dismutase and catalase expression	1645:1712	glutathione peroxidase, superoxide dismutase and catalase expression	1645:1712	Supplementation of SeChNPs at the level of 2 g/kg up-regulated glutathione peroxidase, superoxide dismutase and catalase expression by 1.12, 4.9 and 2.31 folds respectively, in comparison with control group.
33453383	0	111	theme	related	85:91	arg1	genes					93:97	immune related genes	78:97	immune related genes expression	78:108	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	6	112	theme	control	955:961	arg1	group					963:967	The control group	951:967	The control group	951:967	The control group was fed a basal diet (with inorganic se), other groups fed diets supplemented with SeChNPs 0.5, 1 and 2 g/kg diet.
33453383	17	113	theme	stimulatory	2613:2623	arg1	effects					2654:2660	immune stimulatory and free radicals scavenging effects	2606:2660	immune stimulatory and free radicals scavenging effects of selenium loaded chitosan nano composite	2606:2703	In conclusion, the ameliorative effects of SeChNPs on Nile tilapia growth resulted from immune stimulatory and free radicals scavenging effects of selenium loaded chitosan nano composite.
33453383	4	114	theme	apoptotic	740:748	arg1	growth					708:713	the growth	704:713	the growth	704:713	Hence, the current study investigated the effects of selenite-loaded chitosan nanoparticles supplementation on the growth, immune, antioxidant and apoptotic related genes as well as resistance to Aeromonas hydrophila of Nile tilapia, Oreochromis niloticus.
33453383	4	114	theme	apoptotic	740:748	arg1	genes					758:762	related genes	750:762	related genes	750:762	Hence, the current study investigated the effects of selenite-loaded chitosan nanoparticles supplementation on the growth, immune, antioxidant and apoptotic related genes as well as resistance to Aeromonas hydrophila of Nile tilapia, Oreochromis niloticus.
33453383	7	115	theme	Se	1113:1114	arg1	concentration					1096:1108	The loading concentration	1084:1108	The loading concentration of Se to ChNPs	1084:1123	The loading concentration of Se to ChNPs was 0.3, 0.6 and 1.2 mg/0.5, 1 and 2 gm respectively.
33453383	7	115	theme	Se	1113:1114	arg1	mg/0.5					1146:1151	mg/0.5	1146:1151	mg/0.5	1146:1151	The loading concentration of Se to ChNPs was 0.3, 0.6 and 1.2 mg/0.5, 1 and 2 gm respectively.
33453383	13	116	theme	IL-1β	1912:1916	arg1	genes					1918:1922	IL-10, IL-8, TNF-α and IL-1β genes	1889:1922	IL-10, IL-8, TNF-α and IL-1β genes	1889:1922	The expression of IL-10, IL-8, TNF-α and IL-1β genes was up-regulated after dietary inclusion of different levels of SeChNPs in a dose dependent manner.
33453383	0	117	theme	Nile	230:233	arg1	Tilapia					235:241	Nile Tilapia	230:241	Nile Tilapia (Oreochromis niloticus)	230:265	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	0	117	theme	Nile	230:233	arg1	niloticus					256:264	Oreochromis niloticus	244:264	Oreochromis niloticus	244:264	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	17	118	theme	free	2629:2632	arg1	radicals					2634:2641	free radicals	2629:2641	free radicals	2629:2641	In conclusion, the ameliorative effects of SeChNPs on Nile tilapia growth resulted from immune stimulatory and free radicals scavenging effects of selenium loaded chitosan nano composite.
33453383	14	119	theme	Post-challenge	2024:2037	arg1	rate					2061:2064	Post-challenge, the highest survival rate	2024:2064	rate	2061:2064	Post-challenge, the highest survival rate was detected in group fed 2 g/kg SeChNPs (93%) in contrast, the control group was displayed the lowest survival rate (45%).
33453383	10	120	theme	myeloperoxidase	1522:1536	arg1	activities					1538:1547	serum lysozyme, alternative complement and myeloperoxidase activities	1479:1547	serum lysozyme, alternative complement and myeloperoxidase activities	1479:1547	Dietary inclusion of SeChNPs increased serum lysozyme, alternative complement and myeloperoxidase activities and immunoglobulin type M level.
33453383	9	121	theme	control	1425:1431	arg1	group					1433:1437	control group	1425:1437	control group	1425:1437	Additionally, the expression of myostatin gene was down-regulated by 0.2 and 0.3 fold in group fed 0.5 and 1 g/kg SeChNPs when compared with control group.
33453383	4	122	theme	immune	716:721	arg1	growth					708:713	the growth	704:713	the growth	704:713	Hence, the current study investigated the effects of selenite-loaded chitosan nanoparticles supplementation on the growth, immune, antioxidant and apoptotic related genes as well as resistance to Aeromonas hydrophila of Nile tilapia, Oreochromis niloticus.
33453383	4	122	theme	immune	716:721	arg1	genes					758:762	related genes	750:762	related genes	750:762	Hence, the current study investigated the effects of selenite-loaded chitosan nanoparticles supplementation on the growth, immune, antioxidant and apoptotic related genes as well as resistance to Aeromonas hydrophila of Nile tilapia, Oreochromis niloticus.
33453383	2	123	theme	efficient	360:368	arg1	role					370:373	an efficient role	357:373	an efficient role in nutrients and drugs delivery, ponds sterilization, water treatment and aquatic diseases reduction	357:474	It has an efficient role in nutrients and drugs delivery, ponds sterilization, water treatment and aquatic diseases reduction.
33453383	0	124	theme	selenium	15:22	arg1	effect					5:10	Dual effect	0:10	Dual effect of selenium	0:22	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	15	125	theme	SeChNPs	2301:2307	arg1	1					2285:2285	1	2285:2285	1	2285:2285	After challenge with A. hydrophila, the expression of caspase 1 was up-regulated in groups fed 1 and 2 g/kg of SeChNPs.
33453383	15	125	theme	SeChNPs	2301:2307	arg1	SeChNPs					2301:2307	SeChNPs	2301:2307	SeChNPs	2301:2307	After challenge with A. hydrophila, the expression of caspase 1 was up-regulated in groups fed 1 and 2 g/kg of SeChNPs.
33453383	2	126	theme	ponds	408:412	arg1	sterilization					414:426	ponds sterilization	408:426	ponds sterilization	408:426	It has an efficient role in nutrients and drugs delivery, ponds sterilization, water treatment and aquatic diseases reduction.
33453383	4	127	theme	antioxidant	724:734	arg1	growth					708:713	the growth	704:713	the growth	704:713	Hence, the current study investigated the effects of selenite-loaded chitosan nanoparticles supplementation on the growth, immune, antioxidant and apoptotic related genes as well as resistance to Aeromonas hydrophila of Nile tilapia, Oreochromis niloticus.
33453383	4	127	theme	antioxidant	724:734	arg1	genes					758:762	related genes	750:762	related genes	750:762	Hence, the current study investigated the effects of selenite-loaded chitosan nanoparticles supplementation on the growth, immune, antioxidant and apoptotic related genes as well as resistance to Aeromonas hydrophila of Nile tilapia, Oreochromis niloticus.
33453383	15	128	with	challenge	2196:2204	arg1	hydrophila					2214:2223	A. hydrophila	2211:2223	A. hydrophila	2211:2223	After challenge with A. hydrophila, the expression of caspase 1 was up-regulated in groups fed 1 and 2 g/kg of SeChNPs.
33453383	10	129	theme	type	1568:1571	arg1	level					1575:1579	immunoglobulin type M level	1553:1579	immunoglobulin type M level	1553:1579	Dietary inclusion of SeChNPs increased serum lysozyme, alternative complement and myeloperoxidase activities and immunoglobulin type M level.
33453383	0	130	theme	chitosan	31:38	arg1	nanoparticles					40:52	chitosan nanoparticles	31:52	chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus)	31:265	Dual effect of selenium loaded chitosan nanoparticles on growth, antioxidant, immune related genes expression, transcriptomics modulation of caspase 1, cytochrome P450 and heat shock protein and Aeromonas hydrophila resistance of Nile Tilapia (Oreochromis niloticus).
33453383	2	131	theme	aquatic	449:455	arg1	diseases					457:464	aquatic diseases	449:464	aquatic diseases	449:464	It has an efficient role in nutrients and drugs delivery, ponds sterilization, water treatment and aquatic diseases reduction.
33453383	8	132	theme	better	1259:1264	arg1	utilization					1271:1281	better feed utilization	1259:1281	better feed utilization	1259:1281	Fish groups fed SeChNPs (0.5 and 1 g/kg) exhibited the highest final body gain, better feed utilization.
33453383	1	133	theme	great	288:292	arg1	attention					294:302	a great attention	286:302	a great attention for nanotechnology in aquaculture production	286:347	Nowadays there is a great attention for nanotechnology in aquaculture production.
33453383	13	134	theme	genes	1918:1922	arg1	expression					1875:1884	The expression	1871:1884	The expression of IL-10, IL-8, TNF-α and IL-1β genes	1871:1922	The expression of IL-10, IL-8, TNF-α and IL-1β genes was up-regulated after dietary inclusion of different levels of SeChNPs in a dose dependent manner.
33453383	17	135	theme	Nile	2572:2575	arg1	growth					2585:2590	Nile tilapia growth	2572:2590	Nile tilapia growth	2572:2590	In conclusion, the ameliorative effects of SeChNPs on Nile tilapia growth resulted from immune stimulatory and free radicals scavenging effects of selenium loaded chitosan nano composite.
33453383	2	136	dep	delivery	398:405	arg1	reduction					466:474	reduction	466:474	reduction	466:474	It has an efficient role in nutrients and drugs delivery, ponds sterilization, water treatment and aquatic diseases reduction.
33453383	17	137	theme	scavenging	2643:2652	arg1	effects					2654:2660	immune stimulatory and free radicals scavenging effects	2606:2660	immune stimulatory and free radicals scavenging effects of selenium loaded chitosan nano composite	2606:2703	In conclusion, the ameliorative effects of SeChNPs on Nile tilapia growth resulted from immune stimulatory and free radicals scavenging effects of selenium loaded chitosan nano composite.
33453383	8	138	dep	SeChNPs	1195:1201	arg1	0.5					1204:1206	0.5	1204:1206	0.5	1204:1206	Fish groups fed SeChNPs (0.5 and 1 g/kg) exhibited the highest final body gain, better feed utilization.
33453383	8	138	dep	SeChNPs	1195:1201	arg1	1 g/kg					1212:1217	1 g/kg	1212:1217	1 g/kg	1212:1217	Fish groups fed SeChNPs (0.5 and 1 g/kg) exhibited the highest final body gain, better feed utilization.
33453383	6	139	theme	0.5	1060:1062	arg1	diet					1078:1081	SeChNPs 0.5, 1 and 2 g/kg diet	1052:1081	diet	1078:1081	The control group was fed a basal diet (with inorganic se), other groups fed diets supplemented with SeChNPs 0.5, 1 and 2 g/kg diet.
33453383	13	140	theme	dietary	1947:1953	arg1	inclusion					1955:1963	dietary inclusion	1947:1963	dietary inclusion of different levels of SeChNPs	1947:1994	The expression of IL-10, IL-8, TNF-α and IL-1β genes was up-regulated after dietary inclusion of different levels of SeChNPs in a dose dependent manner.
33453383	11	141	theme	SeChNPs	1601:1607	arg1	Supplementation					1582:1596	Supplementation	1582:1596	Supplementation of SeChNPs at the level of 2 g/kg	1582:1630	Supplementation of SeChNPs at the level of 2 g/kg up-regulated glutathione peroxidase, superoxide dismutase and catalase expression by 1.12, 4.9 and 2.31 folds respectively, in comparison with control group.
33453383	16	142	theme	maximum	2324:2330	arg1	down-regulation					2332:2346	the maximum down-regulation	2320:2346	the maximum down-regulation of cytochromes P450 and heat shock protein	2320:2389	Moreover, the maximum down-regulation of cytochromes P450 and heat shock protein were found in 2 g/kg SeChNPs supplemented group (reduced by 0.4 and 0.6-fold, respectively, when compared with control group).
33453383	14	143	theme	2 g/kg	2092:2097	arg1	%					2110:2110	93%	2108:2110	93%	2108:2110	Post-challenge, the highest survival rate was detected in group fed 2 g/kg SeChNPs (93%) in contrast, the control group was displayed the lowest survival rate (45%).
33453383	14	143	theme	2 g/kg	2092:2097	arg1	SeChNPs					2099:2105	2 g/kg SeChNPs	2092:2105	2 g/kg SeChNPs (93%) in contrast, the control group was displayed the lowest survival rate (45%)	2092:2187	Post-challenge, the highest survival rate was detected in group fed 2 g/kg SeChNPs (93%) in contrast, the control group was displayed the lowest survival rate (45%).
33453383	11	144	theme	control	1775:1781	arg1	group					1783:1787	control group	1775:1787	control group	1775:1787	Supplementation of SeChNPs at the level of 2 g/kg up-regulated glutathione peroxidase, superoxide dismutase and catalase expression by 1.12, 4.9 and 2.31 folds respectively, in comparison with control group.
32861200	10	0	theme	resultant	1718:1726	arg1	increases					1728:1736	resultant increases	1718:1736	resultant increases in faecal SCFAs	1718:1752	Prebiotic-related modulation of microbial function and resultant increases in faecal SCFAs were not sufficient to prevent CIH-induced apnoea and hypertension in our model.
32861200	11	1	theme	such	2082:2085	arg1	changes					2087:2093	such changes	2082:2093	such changes	2082:2093	Our results do not exclude the potential for microbiota-gut-brain axis involvement in OSA-related cardiorespiratory morbidity, but they demonstrate that in a relatively mild model of CIH, sufficient to evoke classic cardiorespiratory dysfunction, such changes are not obligatory for the development of morbidity, but may become relevant in the elaboration and maintenance of cardiorespiratory morbidity with progressive disease.
32861200	8	2	theme	microbiota	1264:1273	arg1	composition					1275:1285	gut microbiota composition	1260:1285	gut microbiota composition	1260:1285	Prebiotic administration modulated gut microbiota composition and diversity, altering gut-metabolic (GMMs) and gut-brain (GBMs) modules and increased faecal acetic and propionic acid concentrations, but did not prevent adverse CIH-induced cardiorespiratory phenotypes.
32861200	6	3	theme	Lactobacilli	964:975	arg1	species					977:983	Lactobacilli species	964:983	Lactobacilli species	964:983	CIH exposure had modest effects on the gut microbiota, decreasing the relative abundance of Lactobacilli species, but had no effect on microbial functional characteristics.
32861200	6	4	theme	CIH	872:874	arg1	exposure					876:883	CIH exposure	872:883	CIH exposure	872:883	CIH exposure had modest effects on the gut microbiota, decreasing the relative abundance of Lactobacilli species, but had no effect on microbial functional characteristics.
32861200	2	5	theme	Chronic	333:339	arg1	CIH					363:365	CIH	363:365	CIH	363:365	Chronic intermittent hypoxia (CIH), modelling human sleep apnoea, affects gut microbiota composition and elicits cardiorespiratory morbidity.
32861200	2	5	theme	Chronic	333:339	arg1	apnoea					391:396	modelling human sleep apnoea	369:396	modelling human sleep apnoea	369:396	Chronic intermittent hypoxia (CIH), modelling human sleep apnoea, affects gut microbiota composition and elicits cardiorespiratory morbidity.
32861200	2	5	theme	Chronic	333:339	arg1	hypoxia					354:360	Chronic intermittent hypoxia	333:360	Chronic intermittent hypoxia (CIH)	333:366	Chronic intermittent hypoxia (CIH), modelling human sleep apnoea, affects gut microbiota composition and elicits cardiorespiratory morbidity.
32861200	1	6	theme	cardiorespiratory	303:319	arg1	physiology					321:330	cardiorespiratory physiology	303:330	cardiorespiratory physiology	303:330	BACKGROUND Evidence is accruing to suggest that microbiota-gut-brain signalling plays a regulatory role in cardiorespiratory physiology.
32861200	4	7	theme	prebiotic	702:710	arg1	supplementation					712:726	prebiotic supplementation	702:726	prebiotic supplementation (fructo- and galacto-oligosaccharides) beginning two weeks prior to gas exposures	702:808	METHODS Adult male rats were exposed to CIH (96 cycles/day, 6.0% O2 at nadir) for 14 consecutive days with and without prebiotic supplementation (fructo- and galacto-oligosaccharides) beginning two weeks prior to gas exposures.
32861200	1	8	theme	BACKGROUND	196:205	arg1	Evidence					207:214	BACKGROUND Evidence	196:214	BACKGROUND Evidence	196:214	BACKGROUND Evidence is accruing to suggest that microbiota-gut-brain signalling plays a regulatory role in cardiorespiratory physiology.
32861200	12	9	theme	College	2336:2342	arg1	Cork					2344:2347	University College Cork	2325:2347	University College Cork	2325:2347	FUNDING Department of Physiology and APC Microbiome Ireland, University College Cork, Ireland.
32861200	12	9	theme	College	2336:2342	arg1	Ireland					2316:2322	Ireland	2316:2322	Ireland	2316:2322	FUNDING Department of Physiology and APC Microbiome Ireland, University College Cork, Ireland.
32861200	11	10	from	model	2009:2013	arg1	obligatory					2103:2112	obligatory	2103:2112	obligatory	2103:2112	Our results do not exclude the potential for microbiota-gut-brain axis involvement in OSA-related cardiorespiratory morbidity, but they demonstrate that in a relatively mild model of CIH, sufficient to evoke classic cardiorespiratory dysfunction, such changes are not obligatory for the development of morbidity, but may become relevant in the elaboration and maintenance of cardiorespiratory morbidity with progressive disease.
32861200	10	11	theme	CIH-induced	1785:1795	arg1	apnoea					1797:1802	CIH-induced apnoea	1785:1802	CIH-induced apnoea	1785:1802	Prebiotic-related modulation of microbial function and resultant increases in faecal SCFAs were not sufficient to prevent CIH-induced apnoea and hypertension in our model.
32861200	12	12	theme	University	2325:2334	arg1	Cork					2344:2347	University College Cork	2325:2347	University College Cork	2325:2347	FUNDING Department of Physiology and APC Microbiome Ireland, University College Cork, Ireland.
32861200	12	12	theme	University	2325:2334	arg1	Ireland					2316:2322	Ireland	2316:2322	Ireland	2316:2322	FUNDING Department of Physiology and APC Microbiome Ireland, University College Cork, Ireland.
32861200	0	13	theme	intermittent	128:139	arg1	apnoea					157:162	chronic intermittent hypoxia-induced apnoea	120:162	chronic intermittent hypoxia-induced apnoea	120:162	Prebiotic administration modulates gut microbiota and faecal short-chain fatty acid concentrations but does not prevent chronic intermittent hypoxia-induced apnoea and hypertension in adult rats.
32861200	11	14	theme	progressive	2243:2253	arg1	disease					2255:2261	progressive disease	2243:2261	progressive disease	2243:2261	Our results do not exclude the potential for microbiota-gut-brain axis involvement in OSA-related cardiorespiratory morbidity, but they demonstrate that in a relatively mild model of CIH, sufficient to evoke classic cardiorespiratory dysfunction, such changes are not obligatory for the development of morbidity, but may become relevant in the elaboration and maintenance of cardiorespiratory morbidity with progressive disease.
32861200	9	15	theme	decreased	1625:1633	arg1	concentrations					1647:1660	decreased faecal SCFA concentrations	1625:1660	decreased faecal SCFA concentrations	1625:1660	INTERPRETATION CIH-induced cardiorespiratory dysfunction is not dependant upon changes in microbial functional characteristics and decreased faecal SCFA concentrations.
32861200	6	16	theme	microbial	1007:1015	arg1	characteristics					1028:1042	microbial functional characteristics	1007:1042	microbial functional characteristics	1007:1042	CIH exposure had modest effects on the gut microbiota, decreasing the relative abundance of Lactobacilli species, but had no effect on microbial functional characteristics.
32861200	11	17	from	relevant	2163:2170	arg1	maintenance					2195:2205	maintenance	2195:2205	maintenance	2195:2205	Our results do not exclude the potential for microbiota-gut-brain axis involvement in OSA-related cardiorespiratory morbidity, but they demonstrate that in a relatively mild model of CIH, sufficient to evoke classic cardiorespiratory dysfunction, such changes are not obligatory for the development of morbidity, but may become relevant in the elaboration and maintenance of cardiorespiratory morbidity with progressive disease.
32861200	11	17	from	relevant	2163:2170	arg1	elaboration					2179:2189	elaboration	2179:2189	elaboration	2179:2189	Our results do not exclude the potential for microbiota-gut-brain axis involvement in OSA-related cardiorespiratory morbidity, but they demonstrate that in a relatively mild model of CIH, sufficient to evoke classic cardiorespiratory dysfunction, such changes are not obligatory for the development of morbidity, but may become relevant in the elaboration and maintenance of cardiorespiratory morbidity with progressive disease.
32861200	8	18	theme	faecal	1375:1380	arg1	concentrations					1408:1421	increased faecal acetic and propionic acid concentrations	1365:1421	increased faecal acetic and propionic acid concentrations	1365:1421	Prebiotic administration modulated gut microbiota composition and diversity, altering gut-metabolic (GMMs) and gut-brain (GBMs) modules and increased faecal acetic and propionic acid concentrations, but did not prevent adverse CIH-induced cardiorespiratory phenotypes.
32861200	7	19	theme	cytokine	1136:1143	arg1	concentrations					1145:1158	plasma and brainstem pro-inflammatory cytokine concentrations	1098:1158	plasma and brainstem pro-inflammatory cytokine concentrations	1098:1158	Faecal short-chain fatty acid (SCFA) concentrations, plasma and brainstem pro-inflammatory cytokine concentrations and brainstem neurochemistry were unaffected by exposure to CIH.
32861200	9	20	theme	SCFA	1642:1645	arg1	concentrations					1647:1660	decreased faecal SCFA concentrations	1625:1660	decreased faecal SCFA concentrations	1625:1660	INTERPRETATION CIH-induced cardiorespiratory dysfunction is not dependant upon changes in microbial functional characteristics and decreased faecal SCFA concentrations.
32861200	12	21	theme	APC	2301:2303	arg1	Department					2272:2281	FUNDING Department	2264:2281	FUNDING Department of Physiology and APC	2264:2303	FUNDING Department of Physiology and APC Microbiome Ireland, University College Cork, Ireland.
32861200	11	22	theme	CIH	2018:2020	arg1	model					2009:2013	a relatively mild model	1991:2013	a relatively mild model of CIH	1991:2020	Our results do not exclude the potential for microbiota-gut-brain axis involvement in OSA-related cardiorespiratory morbidity, but they demonstrate that in a relatively mild model of CIH, sufficient to evoke classic cardiorespiratory dysfunction, such changes are not obligatory for the development of morbidity, but may become relevant in the elaboration and maintenance of cardiorespiratory morbidity with progressive disease.
32861200	7	23	theme	brainstem	1109:1117	arg1	concentrations					1145:1158	plasma and brainstem pro-inflammatory cytokine concentrations	1098:1158	plasma and brainstem pro-inflammatory cytokine concentrations	1098:1158	Faecal short-chain fatty acid (SCFA) concentrations, plasma and brainstem pro-inflammatory cytokine concentrations and brainstem neurochemistry were unaffected by exposure to CIH.
32861200	8	24	theme	acid	1403:1406	arg1	concentrations					1408:1421	increased faecal acetic and propionic acid concentrations	1365:1421	increased faecal acetic and propionic acid concentrations	1365:1421	Prebiotic administration modulated gut microbiota composition and diversity, altering gut-metabolic (GMMs) and gut-brain (GBMs) modules and increased faecal acetic and propionic acid concentrations, but did not prevent adverse CIH-induced cardiorespiratory phenotypes.
32861200	7	25	theme	plasma	1098:1103	arg1	concentrations					1145:1158	plasma and brainstem pro-inflammatory cytokine concentrations	1098:1158	plasma and brainstem pro-inflammatory cytokine concentrations	1098:1158	Faecal short-chain fatty acid (SCFA) concentrations, plasma and brainstem pro-inflammatory cytokine concentrations and brainstem neurochemistry were unaffected by exposure to CIH.
32861200	11	26	theme	cardiorespiratory	2210:2226	arg1	morbidity					2228:2236	cardiorespiratory morbidity	2210:2236	cardiorespiratory morbidity	2210:2236	Our results do not exclude the potential for microbiota-gut-brain axis involvement in OSA-related cardiorespiratory morbidity, but they demonstrate that in a relatively mild model of CIH, sufficient to evoke classic cardiorespiratory dysfunction, such changes are not obligatory for the development of morbidity, but may become relevant in the elaboration and maintenance of cardiorespiratory morbidity with progressive disease.
32861200	7	27	theme	fatty	1064:1068	arg1	SCFA					1076:1079	SCFA	1076:1079	SCFA	1076:1079	Faecal short-chain fatty acid (SCFA) concentrations, plasma and brainstem pro-inflammatory cytokine concentrations and brainstem neurochemistry were unaffected by exposure to CIH.
32861200	7	27	theme	fatty	1064:1068	arg1	acid					1070:1073	short-chain fatty acid	1052:1073	Faecal short-chain fatty acid (SCFA) concentrations	1045:1095	Faecal short-chain fatty acid (SCFA) concentrations, plasma and brainstem pro-inflammatory cytokine concentrations and brainstem neurochemistry were unaffected by exposure to CIH.
32861200	4	28	theme	prior	787:791	arg1	weeks					781:785	two weeks	777:785	two weeks prior to gas exposures	777:808	METHODS Adult male rats were exposed to CIH (96 cycles/day, 6.0% O2 at nadir) for 14 consecutive days with and without prebiotic supplementation (fructo- and galacto-oligosaccharides) beginning two weeks prior to gas exposures.
32861200	11	29	theme	morbidity	2137:2145	arg1	development					2122:2132	the development	2118:2132	the development of morbidity	2118:2145	Our results do not exclude the potential for microbiota-gut-brain axis involvement in OSA-related cardiorespiratory morbidity, but they demonstrate that in a relatively mild model of CIH, sufficient to evoke classic cardiorespiratory dysfunction, such changes are not obligatory for the development of morbidity, but may become relevant in the elaboration and maintenance of cardiorespiratory morbidity with progressive disease.
32861200	0	30	theme	fatty	73:77	arg1	acid					79:82	short-chain fatty acid	61:82	short-chain fatty acid	61:82	Prebiotic administration modulates gut microbiota and faecal short-chain fatty acid concentrations but does not prevent chronic intermittent hypoxia-induced apnoea and hypertension in adult rats.
32861200	4	31	dep	CIH	623:625	arg1	%					646:646	96 cycles/day, 6.0% O2	628:649	%	646:646	METHODS Adult male rats were exposed to CIH (96 cycles/day, 6.0% O2 at nadir) for 14 consecutive days with and without prebiotic supplementation (fructo- and galacto-oligosaccharides) beginning two weeks prior to gas exposures.
32861200	7	32	theme	Faecal	1045:1050	arg1	concentrations					1082:1095	Faecal short-chain fatty acid (SCFA) concentrations	1045:1095	Faecal short-chain fatty acid (SCFA) concentrations	1045:1095	Faecal short-chain fatty acid (SCFA) concentrations, plasma and brainstem pro-inflammatory cytokine concentrations and brainstem neurochemistry were unaffected by exposure to CIH.
32861200	0	33	theme	adult	184:188	arg1	rats					190:193	adult rats	184:193	adult rats	184:193	Prebiotic administration modulates gut microbiota and faecal short-chain fatty acid concentrations but does not prevent chronic intermittent hypoxia-induced apnoea and hypertension in adult rats.
32861200	12	34	theme	Physiology	2286:2295	arg1	Department					2272:2281	FUNDING Department	2264:2281	FUNDING Department of Physiology and APC	2264:2303	FUNDING Department of Physiology and APC Microbiome Ireland, University College Cork, Ireland.
32861200	4	35	from	nadir	654:658	arg1	%					646:646	96 cycles/day, 6.0% O2	628:649	%	646:646	METHODS Adult male rats were exposed to CIH (96 cycles/day, 6.0% O2 at nadir) for 14 consecutive days with and without prebiotic supplementation (fructo- and galacto-oligosaccharides) beginning two weeks prior to gas exposures.
32861200	11	36	from	potential	1866:1874	arg1	morbidity					1951:1959	OSA-related cardiorespiratory morbidity	1921:1959	OSA-related cardiorespiratory morbidity	1921:1959	Our results do not exclude the potential for microbiota-gut-brain axis involvement in OSA-related cardiorespiratory morbidity, but they demonstrate that in a relatively mild model of CIH, sufficient to evoke classic cardiorespiratory dysfunction, such changes are not obligatory for the development of morbidity, but may become relevant in the elaboration and maintenance of cardiorespiratory morbidity with progressive disease.
32861200	4	37	theme	male	597:600	arg1	rats					602:605	Adult male rats	591:605	Adult male rats	591:605	METHODS Adult male rats were exposed to CIH (96 cycles/day, 6.0% O2 at nadir) for 14 consecutive days with and without prebiotic supplementation (fructo- and galacto-oligosaccharides) beginning two weeks prior to gas exposures.
32861200	11	38	theme	morbidity	2228:2236	arg1	maintenance					2195:2205	maintenance	2195:2205	maintenance	2195:2205	Our results do not exclude the potential for microbiota-gut-brain axis involvement in OSA-related cardiorespiratory morbidity, but they demonstrate that in a relatively mild model of CIH, sufficient to evoke classic cardiorespiratory dysfunction, such changes are not obligatory for the development of morbidity, but may become relevant in the elaboration and maintenance of cardiorespiratory morbidity with progressive disease.
32861200	11	38	theme	morbidity	2228:2236	arg1	elaboration					2179:2189	elaboration	2179:2189	elaboration	2179:2189	Our results do not exclude the potential for microbiota-gut-brain axis involvement in OSA-related cardiorespiratory morbidity, but they demonstrate that in a relatively mild model of CIH, sufficient to evoke classic cardiorespiratory dysfunction, such changes are not obligatory for the development of morbidity, but may become relevant in the elaboration and maintenance of cardiorespiratory morbidity with progressive disease.
32861200	8	39	theme	CIH-induced	1452:1462	arg1	phenotypes					1482:1491	adverse CIH-induced cardiorespiratory phenotypes	1444:1491	adverse CIH-induced cardiorespiratory phenotypes	1444:1491	Prebiotic administration modulated gut microbiota composition and diversity, altering gut-metabolic (GMMs) and gut-brain (GBMs) modules and increased faecal acetic and propionic acid concentrations, but did not prevent adverse CIH-induced cardiorespiratory phenotypes.
32861200	0	40	theme	acid	79:82	arg1	concentrations					84:97	gut microbiota and faecal short-chain fatty acid concentrations	35:97	concentrations	84:97	Prebiotic administration modulates gut microbiota and faecal short-chain fatty acid concentrations but does not prevent chronic intermittent hypoxia-induced apnoea and hypertension in adult rats.
32861200	4	41	theme	gas	796:798	arg1	exposures					800:808	gas exposures	796:808	gas exposures	796:808	METHODS Adult male rats were exposed to CIH (96 cycles/day, 6.0% O2 at nadir) for 14 consecutive days with and without prebiotic supplementation (fructo- and galacto-oligosaccharides) beginning two weeks prior to gas exposures.
32861200	0	42	theme	Prebiotic	0:8	arg1	administration					10:23	Prebiotic administration	0:23	Prebiotic administration	0:23	Prebiotic administration modulates gut microbiota and faecal short-chain fatty acid concentrations but does not prevent chronic intermittent hypoxia-induced apnoea and hypertension in adult rats.
32861200	3	43	theme	cardiorespiratory	532:548	arg1	dysfunction					550:560	cardiorespiratory dysfunction	532:560	cardiorespiratory dysfunction in CIH-exposed rats	532:580	We investigated if treatment with prebiotics ameliorates cardiorespiratory dysfunction in CIH-exposed rats.
32861200	11	44	from	obligatory	2103:2112	arg1	model					2009:2013	a relatively mild model	1991:2013	a relatively mild model of CIH	1991:2020	Our results do not exclude the potential for microbiota-gut-brain axis involvement in OSA-related cardiorespiratory morbidity, but they demonstrate that in a relatively mild model of CIH, sufficient to evoke classic cardiorespiratory dysfunction, such changes are not obligatory for the development of morbidity, but may become relevant in the elaboration and maintenance of cardiorespiratory morbidity with progressive disease.
32861200	13	45	theme	Development	2457:2467	arg1	Plan					2469:2472	the Government's National Development Plan	2431:2472	the Government's National Development Plan	2431:2472	APC Microbiome Ireland is funded by Science Foundation Ireland, through the Government's National Development Plan.
32861200	9	46	from	changes	1573:1579	arg1	concentrations					1647:1660	decreased faecal SCFA concentrations	1625:1660	decreased faecal SCFA concentrations	1625:1660	INTERPRETATION CIH-induced cardiorespiratory dysfunction is not dependant upon changes in microbial functional characteristics and decreased faecal SCFA concentrations.
32861200	9	46	from	changes	1573:1579	arg1	characteristics					1605:1619	microbial functional characteristics	1584:1619	microbial functional characteristics	1584:1619	INTERPRETATION CIH-induced cardiorespiratory dysfunction is not dependant upon changes in microbial functional characteristics and decreased faecal SCFA concentrations.
32861200	10	47	from	increases	1728:1736	arg1	SCFAs					1748:1752	faecal SCFAs	1741:1752	faecal SCFAs	1741:1752	Prebiotic-related modulation of microbial function and resultant increases in faecal SCFAs were not sufficient to prevent CIH-induced apnoea and hypertension in our model.
32861200	4	48	theme	cycles/day	631:640	arg1	%					646:646	96 cycles/day, 6.0% O2	628:649	%	646:646	METHODS Adult male rats were exposed to CIH (96 cycles/day, 6.0% O2 at nadir) for 14 consecutive days with and without prebiotic supplementation (fructo- and galacto-oligosaccharides) beginning two weeks prior to gas exposures.
32861200	11	49	theme	axis	1901:1904	arg1	involvement					1906:1916	microbiota-gut-brain axis involvement	1880:1916	microbiota-gut-brain axis involvement	1880:1916	Our results do not exclude the potential for microbiota-gut-brain axis involvement in OSA-related cardiorespiratory morbidity, but they demonstrate that in a relatively mild model of CIH, sufficient to evoke classic cardiorespiratory dysfunction, such changes are not obligatory for the development of morbidity, but may become relevant in the elaboration and maintenance of cardiorespiratory morbidity with progressive disease.
32861200	0	50	theme	microbiota	39:48	arg1	concentrations					84:97	gut microbiota and faecal short-chain fatty acid concentrations	35:97	concentrations	84:97	Prebiotic administration modulates gut microbiota and faecal short-chain fatty acid concentrations but does not prevent chronic intermittent hypoxia-induced apnoea and hypertension in adult rats.
32861200	11	51	dep	obligatory	2103:2112	arg1	sufficient					2023:2032	sufficient	2023:2032	sufficient	2023:2032	Our results do not exclude the potential for microbiota-gut-brain axis involvement in OSA-related cardiorespiratory morbidity, but they demonstrate that in a relatively mild model of CIH, sufficient to evoke classic cardiorespiratory dysfunction, such changes are not obligatory for the development of morbidity, but may become relevant in the elaboration and maintenance of cardiorespiratory morbidity with progressive disease.
32861200	2	52	theme	sleep	385:389	arg1	hypoxia					354:360	Chronic intermittent hypoxia	333:360	Chronic intermittent hypoxia (CIH)	333:366	Chronic intermittent hypoxia (CIH), modelling human sleep apnoea, affects gut microbiota composition and elicits cardiorespiratory morbidity.
32861200	2	52	theme	sleep	385:389	arg1	apnoea					391:396	modelling human sleep apnoea	369:396	modelling human sleep apnoea	369:396	Chronic intermittent hypoxia (CIH), modelling human sleep apnoea, affects gut microbiota composition and elicits cardiorespiratory morbidity.
32861200	10	53	theme	microbial	1695:1703	arg1	function					1705:1712	microbial function	1695:1712	microbial function	1695:1712	Prebiotic-related modulation of microbial function and resultant increases in faecal SCFAs were not sufficient to prevent CIH-induced apnoea and hypertension in our model.
32861200	11	54	theme	cardiorespiratory	1933:1949	arg1	morbidity					1951:1959	OSA-related cardiorespiratory morbidity	1921:1959	OSA-related cardiorespiratory morbidity	1921:1959	Our results do not exclude the potential for microbiota-gut-brain axis involvement in OSA-related cardiorespiratory morbidity, but they demonstrate that in a relatively mild model of CIH, sufficient to evoke classic cardiorespiratory dysfunction, such changes are not obligatory for the development of morbidity, but may become relevant in the elaboration and maintenance of cardiorespiratory morbidity with progressive disease.
32861200	6	55	contain	had	990:992	arg1	exposure					876:883	CIH exposure	872:883	CIH exposure	872:883	CIH exposure had modest effects on the gut microbiota, decreasing the relative abundance of Lactobacilli species, but had no effect on microbial functional characteristics.
32861200	6	55	contain	had	990:992	arg2	effect					997:1002	no effect	994:1002	no effect	994:1002	CIH exposure had modest effects on the gut microbiota, decreasing the relative abundance of Lactobacilli species, but had no effect on microbial functional characteristics.
32861200	3	56	from	dysfunction	550:560	arg1	rats					577:580	CIH-exposed rats	565:580	CIH-exposed rats	565:580	We investigated if treatment with prebiotics ameliorates cardiorespiratory dysfunction in CIH-exposed rats.
32861200	2	57	theme	modelling	369:377	arg1	hypoxia					354:360	Chronic intermittent hypoxia	333:360	Chronic intermittent hypoxia (CIH)	333:366	Chronic intermittent hypoxia (CIH), modelling human sleep apnoea, affects gut microbiota composition and elicits cardiorespiratory morbidity.
32861200	2	57	theme	modelling	369:377	arg1	apnoea					391:396	modelling human sleep apnoea	369:396	modelling human sleep apnoea	369:396	Chronic intermittent hypoxia (CIH), modelling human sleep apnoea, affects gut microbiota composition and elicits cardiorespiratory morbidity.
32861200	1	58	theme	microbiota-gut-brain	244:263	arg1	signalling					265:274	microbiota-gut-brain signalling	244:274	microbiota-gut-brain signalling	244:274	BACKGROUND Evidence is accruing to suggest that microbiota-gut-brain signalling plays a regulatory role in cardiorespiratory physiology.
32861200	4	59	dep	supplementation	712:726	arg1	galacto-oligosaccharides					741:764	galacto-oligosaccharides	741:764	galacto-oligosaccharides	741:764	METHODS Adult male rats were exposed to CIH (96 cycles/day, 6.0% O2 at nadir) for 14 consecutive days with and without prebiotic supplementation (fructo- and galacto-oligosaccharides) beginning two weeks prior to gas exposures.
32861200	4	59	dep	supplementation	712:726	arg1	fructo-					729:735	fructo-	729:735	fructo-	729:735	METHODS Adult male rats were exposed to CIH (96 cycles/day, 6.0% O2 at nadir) for 14 consecutive days with and without prebiotic supplementation (fructo- and galacto-oligosaccharides) beginning two weeks prior to gas exposures.
32861200	12	60	theme	FUNDING	2264:2270	arg1	Department					2272:2281	FUNDING Department	2264:2281	FUNDING Department of Physiology and APC	2264:2303	FUNDING Department of Physiology and APC Microbiome Ireland, University College Cork, Ireland.
32861200	0	61	from	apnoea	157:162	arg1	rats					190:193	adult rats	184:193	adult rats	184:193	Prebiotic administration modulates gut microbiota and faecal short-chain fatty acid concentrations but does not prevent chronic intermittent hypoxia-induced apnoea and hypertension in adult rats.
32861200	10	62	theme	faecal	1741:1746	arg1	SCFAs					1748:1752	faecal SCFAs	1741:1752	faecal SCFAs	1741:1752	Prebiotic-related modulation of microbial function and resultant increases in faecal SCFAs were not sufficient to prevent CIH-induced apnoea and hypertension in our model.
32861200	2	63	theme	gut	407:409	arg1	composition					422:432	gut microbiota composition	407:432	gut microbiota composition	407:432	Chronic intermittent hypoxia (CIH), modelling human sleep apnoea, affects gut microbiota composition and elicits cardiorespiratory morbidity.
32861200	9	64	theme	microbial	1584:1592	arg1	characteristics					1605:1619	microbial functional characteristics	1584:1619	microbial functional characteristics	1584:1619	INTERPRETATION CIH-induced cardiorespiratory dysfunction is not dependant upon changes in microbial functional characteristics and decreased faecal SCFA concentrations.
32861200	11	65	from	maintenance	2195:2205	arg1	relevant					2163:2170	relevant	2163:2170	relevant	2163:2170	Our results do not exclude the potential for microbiota-gut-brain axis involvement in OSA-related cardiorespiratory morbidity, but they demonstrate that in a relatively mild model of CIH, sufficient to evoke classic cardiorespiratory dysfunction, such changes are not obligatory for the development of morbidity, but may become relevant in the elaboration and maintenance of cardiorespiratory morbidity with progressive disease.
32861200	1	66	theme	regulatory	284:293	arg1	role					295:298	a regulatory role	282:298	a regulatory role	282:298	BACKGROUND Evidence is accruing to suggest that microbiota-gut-brain signalling plays a regulatory role in cardiorespiratory physiology.
32861200	6	67	theme	species	977:983	arg1	abundance					951:959	the relative abundance	938:959	the relative abundance of Lactobacilli species	938:983	CIH exposure had modest effects on the gut microbiota, decreasing the relative abundance of Lactobacilli species, but had no effect on microbial functional characteristics.
32861200	2	68	theme	intermittent	341:352	arg1	CIH					363:365	CIH	363:365	CIH	363:365	Chronic intermittent hypoxia (CIH), modelling human sleep apnoea, affects gut microbiota composition and elicits cardiorespiratory morbidity.
32861200	2	68	theme	intermittent	341:352	arg1	apnoea					391:396	modelling human sleep apnoea	369:396	modelling human sleep apnoea	369:396	Chronic intermittent hypoxia (CIH), modelling human sleep apnoea, affects gut microbiota composition and elicits cardiorespiratory morbidity.
32861200	2	68	theme	intermittent	341:352	arg1	hypoxia					354:360	Chronic intermittent hypoxia	333:360	Chronic intermittent hypoxia (CIH)	333:366	Chronic intermittent hypoxia (CIH), modelling human sleep apnoea, affects gut microbiota composition and elicits cardiorespiratory morbidity.
32861200	8	69	theme	gut	1260:1262	arg1	composition					1275:1285	gut microbiota composition	1260:1285	gut microbiota composition	1260:1285	Prebiotic administration modulated gut microbiota composition and diversity, altering gut-metabolic (GMMs) and gut-brain (GBMs) modules and increased faecal acetic and propionic acid concentrations, but did not prevent adverse CIH-induced cardiorespiratory phenotypes.
32861200	0	70	from	hypertension	168:179	arg1	rats					190:193	adult rats	184:193	adult rats	184:193	Prebiotic administration modulates gut microbiota and faecal short-chain fatty acid concentrations but does not prevent chronic intermittent hypoxia-induced apnoea and hypertension in adult rats.
32861200	11	71	theme	cardiorespiratory	2051:2067	arg1	dysfunction					2069:2079	classic cardiorespiratory dysfunction	2043:2079	classic cardiorespiratory dysfunction	2043:2079	Our results do not exclude the potential for microbiota-gut-brain axis involvement in OSA-related cardiorespiratory morbidity, but they demonstrate that in a relatively mild model of CIH, sufficient to evoke classic cardiorespiratory dysfunction, such changes are not obligatory for the development of morbidity, but may become relevant in the elaboration and maintenance of cardiorespiratory morbidity with progressive disease.
32861200	6	72	theme	relative	942:949	arg1	abundance					951:959	the relative abundance	938:959	the relative abundance of Lactobacilli species	938:983	CIH exposure had modest effects on the gut microbiota, decreasing the relative abundance of Lactobacilli species, but had no effect on microbial functional characteristics.
32861200	11	73	from	elaboration	2179:2189	arg1	relevant					2163:2170	relevant	2163:2170	relevant	2163:2170	Our results do not exclude the potential for microbiota-gut-brain axis involvement in OSA-related cardiorespiratory morbidity, but they demonstrate that in a relatively mild model of CIH, sufficient to evoke classic cardiorespiratory dysfunction, such changes are not obligatory for the development of morbidity, but may become relevant in the elaboration and maintenance of cardiorespiratory morbidity with progressive disease.
32861200	8	74	theme	gut-brain	1336:1344	arg1	GBMs					1347:1350	GBMs	1347:1350	GBMs	1347:1350	Prebiotic administration modulated gut microbiota composition and diversity, altering gut-metabolic (GMMs) and gut-brain (GBMs) modules and increased faecal acetic and propionic acid concentrations, but did not prevent adverse CIH-induced cardiorespiratory phenotypes.
32861200	8	74	theme	gut-brain	1336:1344	arg1	modules					1353:1359	gut-metabolic (GMMs) and gut-brain (GBMs) modules	1311:1359	modules	1353:1359	Prebiotic administration modulated gut microbiota composition and diversity, altering gut-metabolic (GMMs) and gut-brain (GBMs) modules and increased faecal acetic and propionic acid concentrations, but did not prevent adverse CIH-induced cardiorespiratory phenotypes.
32861200	5	75	theme	apnoea	834:839	arg1	index					841:845	apnoea index	834:845	apnoea index	834:845	FINDINGS CIH increased apnoea index and caused hypertension.
32861200	7	76	theme	brainstem	1164:1172	arg1	neurochemistry					1174:1187	brainstem neurochemistry	1164:1187	brainstem neurochemistry	1164:1187	Faecal short-chain fatty acid (SCFA) concentrations, plasma and brainstem pro-inflammatory cytokine concentrations and brainstem neurochemistry were unaffected by exposure to CIH.
32861200	9	77	theme	functional	1594:1603	arg1	characteristics					1605:1619	microbial functional characteristics	1584:1619	microbial functional characteristics	1584:1619	INTERPRETATION CIH-induced cardiorespiratory dysfunction is not dependant upon changes in microbial functional characteristics and decreased faecal SCFA concentrations.
32861200	0	78	theme	hypoxia-induced	141:155	arg1	apnoea					157:162	chronic intermittent hypoxia-induced apnoea	120:162	chronic intermittent hypoxia-induced apnoea	120:162	Prebiotic administration modulates gut microbiota and faecal short-chain fatty acid concentrations but does not prevent chronic intermittent hypoxia-induced apnoea and hypertension in adult rats.
32861200	6	79	theme	functional	1017:1026	arg1	characteristics					1028:1042	microbial functional characteristics	1007:1042	microbial functional characteristics	1007:1042	CIH exposure had modest effects on the gut microbiota, decreasing the relative abundance of Lactobacilli species, but had no effect on microbial functional characteristics.
32861200	4	80	theme	consecutive	668:678	arg1	days					680:683	14 consecutive days	665:683	14 consecutive days with and without prebiotic supplementation (fructo- and galacto-oligosaccharides) beginning two weeks prior to gas exposures	665:808	METHODS Adult male rats were exposed to CIH (96 cycles/day, 6.0% O2 at nadir) for 14 consecutive days with and without prebiotic supplementation (fructo- and galacto-oligosaccharides) beginning two weeks prior to gas exposures.
32861200	11	81	theme	mild	2004:2007	arg1	model					2009:2013	a relatively mild model	1991:2013	a relatively mild model of CIH	1991:2020	Our results do not exclude the potential for microbiota-gut-brain axis involvement in OSA-related cardiorespiratory morbidity, but they demonstrate that in a relatively mild model of CIH, sufficient to evoke classic cardiorespiratory dysfunction, such changes are not obligatory for the development of morbidity, but may become relevant in the elaboration and maintenance of cardiorespiratory morbidity with progressive disease.
32861200	7	82	theme	pro-inflammatory	1119:1134	arg1	concentrations					1145:1158	plasma and brainstem pro-inflammatory cytokine concentrations	1098:1158	plasma and brainstem pro-inflammatory cytokine concentrations	1098:1158	Faecal short-chain fatty acid (SCFA) concentrations, plasma and brainstem pro-inflammatory cytokine concentrations and brainstem neurochemistry were unaffected by exposure to CIH.
32861200	9	83	theme	faecal	1635:1640	arg1	concentrations					1647:1660	decreased faecal SCFA concentrations	1625:1660	decreased faecal SCFA concentrations	1625:1660	INTERPRETATION CIH-induced cardiorespiratory dysfunction is not dependant upon changes in microbial functional characteristics and decreased faecal SCFA concentrations.
32861200	4	84	dep	METHODS	583:589	arg1	exposed					612:618	exposed	612:618	were exposed to CIH (96 cycles/day, 6.0% O2 at nadir) for 14 consecutive days with and without prebiotic supplementation (fructo- and galacto-oligosaccharides) beginning two weeks prior to gas exposures	607:808	METHODS Adult male rats were exposed to CIH (96 cycles/day, 6.0% O2 at nadir) for 14 consecutive days with and without prebiotic supplementation (fructo- and galacto-oligosaccharides) beginning two weeks prior to gas exposures.
32861200	8	85	theme	acetic	1382:1387	arg1	concentrations					1408:1421	increased faecal acetic and propionic acid concentrations	1365:1421	increased faecal acetic and propionic acid concentrations	1365:1421	Prebiotic administration modulated gut microbiota composition and diversity, altering gut-metabolic (GMMs) and gut-brain (GBMs) modules and increased faecal acetic and propionic acid concentrations, but did not prevent adverse CIH-induced cardiorespiratory phenotypes.
32861200	3	86	with	treatment	494:502	arg1	prebiotics					509:518	prebiotics	509:518	prebiotics	509:518	We investigated if treatment with prebiotics ameliorates cardiorespiratory dysfunction in CIH-exposed rats.
32861200	8	87	theme	propionic	1393:1401	arg1	concentrations					1408:1421	increased faecal acetic and propionic acid concentrations	1365:1421	increased faecal acetic and propionic acid concentrations	1365:1421	Prebiotic administration modulated gut microbiota composition and diversity, altering gut-metabolic (GMMs) and gut-brain (GBMs) modules and increased faecal acetic and propionic acid concentrations, but did not prevent adverse CIH-induced cardiorespiratory phenotypes.
32861200	5	88	theme	FINDINGS	811:818	arg1	CIH					820:822	FINDINGS CIH	811:822	FINDINGS CIH	811:822	FINDINGS CIH increased apnoea index and caused hypertension.
32861200	7	89	theme	acid	1070:1073	arg1	concentrations					1082:1095	Faecal short-chain fatty acid (SCFA) concentrations	1045:1095	Faecal short-chain fatty acid (SCFA) concentrations	1045:1095	Faecal short-chain fatty acid (SCFA) concentrations, plasma and brainstem pro-inflammatory cytokine concentrations and brainstem neurochemistry were unaffected by exposure to CIH.
32861200	10	90	from	modulation	1681:1690	arg1	SCFAs					1748:1752	faecal SCFAs	1741:1752	faecal SCFAs	1741:1752	Prebiotic-related modulation of microbial function and resultant increases in faecal SCFAs were not sufficient to prevent CIH-induced apnoea and hypertension in our model.
32861200	4	91	theme	Adult	591:595	arg1	rats					602:605	Adult male rats	591:605	Adult male rats	591:605	METHODS Adult male rats were exposed to CIH (96 cycles/day, 6.0% O2 at nadir) for 14 consecutive days with and without prebiotic supplementation (fructo- and galacto-oligosaccharides) beginning two weeks prior to gas exposures.
32861200	9	92	theme	CIH-induced	1509:1519	arg1	dysfunction					1539:1549	INTERPRETATION CIH-induced cardiorespiratory dysfunction	1494:1549	INTERPRETATION CIH-induced cardiorespiratory dysfunction	1494:1549	INTERPRETATION CIH-induced cardiorespiratory dysfunction is not dependant upon changes in microbial functional characteristics and decreased faecal SCFA concentrations.
32861200	11	93	with	elaboration	2179:2189	arg1	disease					2255:2261	progressive disease	2243:2261	progressive disease	2243:2261	Our results do not exclude the potential for microbiota-gut-brain axis involvement in OSA-related cardiorespiratory morbidity, but they demonstrate that in a relatively mild model of CIH, sufficient to evoke classic cardiorespiratory dysfunction, such changes are not obligatory for the development of morbidity, but may become relevant in the elaboration and maintenance of cardiorespiratory morbidity with progressive disease.
32861200	7	94	theme	short-chain	1052:1062	arg1	SCFA					1076:1079	SCFA	1076:1079	SCFA	1076:1079	Faecal short-chain fatty acid (SCFA) concentrations, plasma and brainstem pro-inflammatory cytokine concentrations and brainstem neurochemistry were unaffected by exposure to CIH.
32861200	7	94	theme	short-chain	1052:1062	arg1	acid					1070:1073	short-chain fatty acid	1052:1073	Faecal short-chain fatty acid (SCFA) concentrations	1045:1095	Faecal short-chain fatty acid (SCFA) concentrations, plasma and brainstem pro-inflammatory cytokine concentrations and brainstem neurochemistry were unaffected by exposure to CIH.
32861200	6	95	contain	had	885:887	arg1	exposure					876:883	CIH exposure	872:883	CIH exposure	872:883	CIH exposure had modest effects on the gut microbiota, decreasing the relative abundance of Lactobacilli species, but had no effect on microbial functional characteristics.
32861200	6	95	contain	had	885:887	arg2	effects					896:902	modest effects	889:902	modest effects	889:902	CIH exposure had modest effects on the gut microbiota, decreasing the relative abundance of Lactobacilli species, but had no effect on microbial functional characteristics.
32861200	11	96	theme	classic	2043:2049	arg1	dysfunction					2069:2079	classic cardiorespiratory dysfunction	2043:2079	classic cardiorespiratory dysfunction	2043:2079	Our results do not exclude the potential for microbiota-gut-brain axis involvement in OSA-related cardiorespiratory morbidity, but they demonstrate that in a relatively mild model of CIH, sufficient to evoke classic cardiorespiratory dysfunction, such changes are not obligatory for the development of morbidity, but may become relevant in the elaboration and maintenance of cardiorespiratory morbidity with progressive disease.
32861200	0	97	theme	chronic	120:126	arg1	apnoea					157:162	chronic intermittent hypoxia-induced apnoea	120:162	chronic intermittent hypoxia-induced apnoea	120:162	Prebiotic administration modulates gut microbiota and faecal short-chain fatty acid concentrations but does not prevent chronic intermittent hypoxia-induced apnoea and hypertension in adult rats.
32861200	8	98	theme	adverse	1444:1450	arg1	phenotypes					1482:1491	adverse CIH-induced cardiorespiratory phenotypes	1444:1491	adverse CIH-induced cardiorespiratory phenotypes	1444:1491	Prebiotic administration modulated gut microbiota composition and diversity, altering gut-metabolic (GMMs) and gut-brain (GBMs) modules and increased faecal acetic and propionic acid concentrations, but did not prevent adverse CIH-induced cardiorespiratory phenotypes.
32861200	12	99	dep	Ireland	2316:2322	arg1	Department					2272:2281	FUNDING Department	2264:2281	FUNDING Department of Physiology and APC	2264:2303	FUNDING Department of Physiology and APC Microbiome Ireland, University College Cork, Ireland.
32861200	8	100	theme	cardiorespiratory	1464:1480	arg1	phenotypes					1482:1491	adverse CIH-induced cardiorespiratory phenotypes	1444:1491	adverse CIH-induced cardiorespiratory phenotypes	1444:1491	Prebiotic administration modulated gut microbiota composition and diversity, altering gut-metabolic (GMMs) and gut-brain (GBMs) modules and increased faecal acetic and propionic acid concentrations, but did not prevent adverse CIH-induced cardiorespiratory phenotypes.
32861200	2	101	theme	microbiota	411:420	arg1	composition					422:432	gut microbiota composition	407:432	gut microbiota composition	407:432	Chronic intermittent hypoxia (CIH), modelling human sleep apnoea, affects gut microbiota composition and elicits cardiorespiratory morbidity.
32861200	0	102	theme	gut	35:37	arg1	concentrations					84:97	gut microbiota and faecal short-chain fatty acid concentrations	35:97	concentrations	84:97	Prebiotic administration modulates gut microbiota and faecal short-chain fatty acid concentrations but does not prevent chronic intermittent hypoxia-induced apnoea and hypertension in adult rats.
32861200	13	103	theme	National	2448:2455	arg1	Plan					2469:2472	the Government's National Development Plan	2431:2472	the Government's National Development Plan	2431:2472	APC Microbiome Ireland is funded by Science Foundation Ireland, through the Government's National Development Plan.
32861200	10	104	theme	Prebiotic-related	1663:1679	arg1	modulation					1681:1690	Prebiotic-related modulation	1663:1690	Prebiotic-related modulation of microbial function	1663:1712	Prebiotic-related modulation of microbial function and resultant increases in faecal SCFAs were not sufficient to prevent CIH-induced apnoea and hypertension in our model.
32861200	11	105	theme	microbiota-gut-brain	1880:1899	arg1	involvement					1906:1916	microbiota-gut-brain axis involvement	1880:1916	microbiota-gut-brain axis involvement	1880:1916	Our results do not exclude the potential for microbiota-gut-brain axis involvement in OSA-related cardiorespiratory morbidity, but they demonstrate that in a relatively mild model of CIH, sufficient to evoke classic cardiorespiratory dysfunction, such changes are not obligatory for the development of morbidity, but may become relevant in the elaboration and maintenance of cardiorespiratory morbidity with progressive disease.
32861200	11	106	with	maintenance	2195:2205	arg1	disease					2255:2261	progressive disease	2243:2261	progressive disease	2243:2261	Our results do not exclude the potential for microbiota-gut-brain axis involvement in OSA-related cardiorespiratory morbidity, but they demonstrate that in a relatively mild model of CIH, sufficient to evoke classic cardiorespiratory dysfunction, such changes are not obligatory for the development of morbidity, but may become relevant in the elaboration and maintenance of cardiorespiratory morbidity with progressive disease.
32861200	11	107	dep	elaboration	2179:2189	arg1	the					2175:2177	the	2175:2177	the	2175:2177	Our results do not exclude the potential for microbiota-gut-brain axis involvement in OSA-related cardiorespiratory morbidity, but they demonstrate that in a relatively mild model of CIH, sufficient to evoke classic cardiorespiratory dysfunction, such changes are not obligatory for the development of morbidity, but may become relevant in the elaboration and maintenance of cardiorespiratory morbidity with progressive disease.
32861200	2	108	theme	cardiorespiratory	446:462	arg1	morbidity					464:472	cardiorespiratory morbidity	446:472	cardiorespiratory morbidity	446:472	Chronic intermittent hypoxia (CIH), modelling human sleep apnoea, affects gut microbiota composition and elicits cardiorespiratory morbidity.
32861200	0	109	theme	short-chain	61:71	arg1	acid					79:82	short-chain fatty acid	61:82	short-chain fatty acid	61:82	Prebiotic administration modulates gut microbiota and faecal short-chain fatty acid concentrations but does not prevent chronic intermittent hypoxia-induced apnoea and hypertension in adult rats.
32861200	6	110	theme	gut	911:913	arg1	microbiota					915:924	the gut microbiota	907:924	the gut microbiota	907:924	CIH exposure had modest effects on the gut microbiota, decreasing the relative abundance of Lactobacilli species, but had no effect on microbial functional characteristics.
32861200	10	111	theme	function	1705:1712	arg1	modulation					1681:1690	Prebiotic-related modulation	1663:1690	Prebiotic-related modulation of microbial function	1663:1712	Prebiotic-related modulation of microbial function and resultant increases in faecal SCFAs were not sufficient to prevent CIH-induced apnoea and hypertension in our model.
32861200	10	111	theme	function	1705:1712	arg1	increases					1728:1736	resultant increases	1718:1736	resultant increases in faecal SCFAs	1718:1752	Prebiotic-related modulation of microbial function and resultant increases in faecal SCFAs were not sufficient to prevent CIH-induced apnoea and hypertension in our model.
32861200	11	112	theme	OSA-related	1921:1931	arg1	morbidity					1951:1959	OSA-related cardiorespiratory morbidity	1921:1959	OSA-related cardiorespiratory morbidity	1921:1959	Our results do not exclude the potential for microbiota-gut-brain axis involvement in OSA-related cardiorespiratory morbidity, but they demonstrate that in a relatively mild model of CIH, sufficient to evoke classic cardiorespiratory dysfunction, such changes are not obligatory for the development of morbidity, but may become relevant in the elaboration and maintenance of cardiorespiratory morbidity with progressive disease.
32861200	2	113	theme	human	379:383	arg1	hypoxia					354:360	Chronic intermittent hypoxia	333:360	Chronic intermittent hypoxia (CIH)	333:366	Chronic intermittent hypoxia (CIH), modelling human sleep apnoea, affects gut microbiota composition and elicits cardiorespiratory morbidity.
32861200	2	113	theme	human	379:383	arg1	apnoea					391:396	modelling human sleep apnoea	369:396	modelling human sleep apnoea	369:396	Chronic intermittent hypoxia (CIH), modelling human sleep apnoea, affects gut microbiota composition and elicits cardiorespiratory morbidity.
32861200	9	114	theme	INTERPRETATION	1494:1507	arg1	dysfunction					1539:1549	INTERPRETATION CIH-induced cardiorespiratory dysfunction	1494:1549	INTERPRETATION CIH-induced cardiorespiratory dysfunction	1494:1549	INTERPRETATION CIH-induced cardiorespiratory dysfunction is not dependant upon changes in microbial functional characteristics and decreased faecal SCFA concentrations.
32861200	8	115	theme	increased	1365:1373	arg1	concentrations					1408:1421	increased faecal acetic and propionic acid concentrations	1365:1421	increased faecal acetic and propionic acid concentrations	1365:1421	Prebiotic administration modulated gut microbiota composition and diversity, altering gut-metabolic (GMMs) and gut-brain (GBMs) modules and increased faecal acetic and propionic acid concentrations, but did not prevent adverse CIH-induced cardiorespiratory phenotypes.
32861200	3	116	theme	CIH-exposed	565:575	arg1	rats					577:580	CIH-exposed rats	565:580	CIH-exposed rats	565:580	We investigated if treatment with prebiotics ameliorates cardiorespiratory dysfunction in CIH-exposed rats.
32861200	9	117	theme	cardiorespiratory	1521:1537	arg1	dysfunction					1539:1549	INTERPRETATION CIH-induced cardiorespiratory dysfunction	1494:1549	INTERPRETATION CIH-induced cardiorespiratory dysfunction	1494:1549	INTERPRETATION CIH-induced cardiorespiratory dysfunction is not dependant upon changes in microbial functional characteristics and decreased faecal SCFA concentrations.
32861200	8	118	theme	Prebiotic	1225:1233	arg1	administration					1235:1248	Prebiotic administration	1225:1248	Prebiotic administration	1225:1248	Prebiotic administration modulated gut microbiota composition and diversity, altering gut-metabolic (GMMs) and gut-brain (GBMs) modules and increased faecal acetic and propionic acid concentrations, but did not prevent adverse CIH-induced cardiorespiratory phenotypes.
32861200	6	119	theme	modest	889:894	arg1	effects					896:902	modest effects	889:902	modest effects	889:902	CIH exposure had modest effects on the gut microbiota, decreasing the relative abundance of Lactobacilli species, but had no effect on microbial functional characteristics.
34516313	4	0	theme	saccharide	1065:1074	arg1	chains					1076:1081	long saccharide chains	1060:1081	long saccharide chains	1060:1081	Motor cortex was found dominated by complex structures with a sialylation degree ≥3, exhibiting long saccharide chains, in the G1 class.
34516313	3	1	theme	high-resolution	696:710	arg1	spectrometry					717:728	high-resolution mass spectrometry	696:728	high-resolution mass spectrometry mode	696:733	The spectral data, acquired in high-resolution mass spectrometry mode with a remarkable sensitivity and an average mass accuracy of 4.48 ppm, also show that the gangliosidome of motor cortex is generally characterized by species exhibiting a much higher degree of sialylation than previously known.
34516313	6	2	theme	longest	1459:1465	arg1	chain					1483:1487	the longest oligosaccharide chain	1455:1487	the longest oligosaccharide chain discovered so far in the human brain	1455:1524	Of major significance is the identification of hepta and octasialylated species of GS1 and GO1 type, which are among the structures with the longest oligosaccharide chain discovered so far in the human brain.
34516313	1	3	theme	gangliosides	281:292	arg1	screening					241:249	screening	241:249	screening	241:249	We have developed here a superior methodology based on high-resolution mass spectrometry for screening and fragmentation analysis of gangliosides extracted and purified from the human motor cortex .
34516313	1	3	theme	gangliosides	281:292	arg1	analysis					269:276	fragmentation analysis	255:276	fragmentation analysis	255:276	We have developed here a superior methodology based on high-resolution mass spectrometry for screening and fragmentation analysis of gangliosides extracted and purified from the human motor cortex .
34516313	2	4	theme	structures	614:623	arg1	number					604:609	the highest number	592:609	the highest number of structures identified so far in this brain region	592:662	The experiments, conducted on a nanoelectrospray Orbitrap mass spectroscope in the negative ion mode, allowed the discrimination in the native mixture extracted from human motor cortex of no less than 83 different gangliosides, which represents the highest number of structures identified so far in this brain region.
34516313	3	5	theme	remarkable	742:751	arg1	sensitivity					753:763	remarkable sensitivity	742:763	remarkable sensitivity	742:763	The spectral data, acquired in high-resolution mass spectrometry mode with a remarkable sensitivity and an average mass accuracy of 4.48 ppm, also show that the gangliosidome of motor cortex is generally characterized by species exhibiting a much higher degree of sialylation than previously known.
34516313	2	6	theme	brain	651:655	arg1	region					657:662	this brain region	646:662	this brain region	646:662	The experiments, conducted on a nanoelectrospray Orbitrap mass spectroscope in the negative ion mode, allowed the discrimination in the native mixture extracted from human motor cortex of no less than 83 different gangliosides, which represents the highest number of structures identified so far in this brain region.
34516313	3	7	theme	average	772:778	arg1	accuracy					785:792	an average mass accuracy	769:792	an average mass accuracy	769:792	The spectral data, acquired in high-resolution mass spectrometry mode with a remarkable sensitivity and an average mass accuracy of 4.48 ppm, also show that the gangliosidome of motor cortex is generally characterized by species exhibiting a much higher degree of sialylation than previously known.
34516313	0	8	theme	motor	134:138	arg1	cortex					140:145	the human motor cortex	124:145	the human motor cortex	124:145	High-resolution mass spectrometry reveals a complex ganglioside pattern and novel polysialylated structures associated with the human motor cortex.
34516313	6	9	theme	human	1514:1518	arg1	brain					1520:1524	the human brain	1510:1524	the human brain	1510:1524	Of major significance is the identification of hepta and octasialylated species of GS1 and GO1 type, which are among the structures with the longest oligosaccharide chain discovered so far in the human brain.
34516313	1	10	theme	mass	219:222	arg1	spectrometry					224:235	high-resolution mass spectrometry	203:235	high-resolution mass spectrometry for screening and fragmentation analysis of gangliosides extracted and purified from the human motor cortex	203:343	We have developed here a superior methodology based on high-resolution mass spectrometry for screening and fragmentation analysis of gangliosides extracted and purified from the human motor cortex .
34516313	7	11	theme	structural	1642:1651	arg1	data					1653:1656	structural data	1642:1656	structural data documenting the occurrence of GT1b (d18:1/20:0) isomer in the human motor cortex	1642:1737	In the last stage of research, tandem mass spectrometry performed by higher energy collision dissociation provided structural data documenting the occurrence of GT1b (d18:1/20:0) isomer in the human motor cortex.
34516313	2	12	theme	gangliosides	561:572	arg1	cortex					525:530	human motor cortex	513:530	human motor cortex of no less than 83 different gangliosides, which represents the highest number of structures identified so far in this brain region	513:662	The experiments, conducted on a nanoelectrospray Orbitrap mass spectroscope in the negative ion mode, allowed the discrimination in the native mixture extracted from human motor cortex of no less than 83 different gangliosides, which represents the highest number of structures identified so far in this brain region.
34516313	3	13	theme	4.48 ppm	797:804	arg1	accuracy					785:792	an average mass accuracy	769:792	an average mass accuracy	769:792	The spectral data, acquired in high-resolution mass spectrometry mode with a remarkable sensitivity and an average mass accuracy of 4.48 ppm, also show that the gangliosidome of motor cortex is generally characterized by species exhibiting a much higher degree of sialylation than previously known.
34516313	3	13	theme	4.48 ppm	797:804	arg1	sensitivity					753:763	remarkable sensitivity	742:763	remarkable sensitivity	742:763	The spectral data, acquired in high-resolution mass spectrometry mode with a remarkable sensitivity and an average mass accuracy of 4.48 ppm, also show that the gangliosidome of motor cortex is generally characterized by species exhibiting a much higher degree of sialylation than previously known.
34516313	6	14	theme	major	1321:1325	arg1	significance					1327:1338	major significance	1321:1338	major significance	1321:1338	Of major significance is the identification of hepta and octasialylated species of GS1 and GO1 type, which are among the structures with the longest oligosaccharide chain discovered so far in the human brain.
34516313	7	15	theme	energy	1603:1608	arg1	dissociation					1620:1631	higher energy collision dissociation	1596:1631	higher energy collision dissociation	1596:1631	In the last stage of research, tandem mass spectrometry performed by higher energy collision dissociation provided structural data documenting the occurrence of GT1b (d18:1/20:0) isomer in the human motor cortex.
34516313	1	16	dep	gangliosides	281:292	arg1	purified					308:315	purified	308:315	purified from the human motor cortex	308:343	We have developed here a superior methodology based on high-resolution mass spectrometry for screening and fragmentation analysis of gangliosides extracted and purified from the human motor cortex .
34516313	1	16	dep	gangliosides	281:292	arg1	extracted					294:302	extracted	294:302	extracted	294:302	We have developed here a superior methodology based on high-resolution mass spectrometry for screening and fragmentation analysis of gangliosides extracted and purified from the human motor cortex .
34516313	7	17	theme	human	1720:1724	arg1	cortex					1732:1737	the human motor cortex	1716:1737	the human motor cortex	1716:1737	In the last stage of research, tandem mass spectrometry performed by higher energy collision dissociation provided structural data documenting the occurrence of GT1b (d18:1/20:0) isomer in the human motor cortex.
34516313	2	18	theme	motor	519:523	arg1	cortex					525:530	human motor cortex	513:530	human motor cortex of no less than 83 different gangliosides, which represents the highest number of structures identified so far in this brain region	513:662	The experiments, conducted on a nanoelectrospray Orbitrap mass spectroscope in the negative ion mode, allowed the discrimination in the native mixture extracted from human motor cortex of no less than 83 different gangliosides, which represents the highest number of structures identified so far in this brain region.
34516313	5	19	theme	first	1274:1278	arg1	time					1280:1283	the first time	1270:1283	the first time discovered in this brain region	1270:1315	Fucogangliosides and species with the glycan chain elongated by either O-acetylation and/or acetate anion attachments were also detected; the later modification was for the first time discovered in this brain region.
34516313	3	20	theme	motor	843:847	arg1	cortex					849:854	motor cortex	843:854	motor cortex	843:854	The spectral data, acquired in high-resolution mass spectrometry mode with a remarkable sensitivity and an average mass accuracy of 4.48 ppm, also show that the gangliosidome of motor cortex is generally characterized by species exhibiting a much higher degree of sialylation than previously known.
34516313	0	21	theme	polysialylated	82:95	arg1	structures					97:106	novel polysialylated structures	76:106	novel polysialylated structures	76:106	High-resolution mass spectrometry reveals a complex ganglioside pattern and novel polysialylated structures associated with the human motor cortex.
34516313	7	22	theme	tandem	1558:1563	arg1	spectrometry					1570:1581	tandem mass spectrometry	1558:1581	tandem mass spectrometry performed by higher energy collision dissociation	1558:1631	In the last stage of research, tandem mass spectrometry performed by higher energy collision dissociation provided structural data documenting the occurrence of GT1b (d18:1/20:0) isomer in the human motor cortex.
34516313	6	23	theme	octasialylated	1375:1388	arg1	species					1390:1396	octasialylated species	1375:1396	octasialylated species	1375:1396	Of major significance is the identification of hepta and octasialylated species of GS1 and GO1 type, which are among the structures with the longest oligosaccharide chain discovered so far in the human brain.
34516313	7	24	theme	research	1548:1555	arg1	stage					1539:1543	the last stage	1530:1543	the last stage of research	1530:1555	In the last stage of research, tandem mass spectrometry performed by higher energy collision dissociation provided structural data documenting the occurrence of GT1b (d18:1/20:0) isomer in the human motor cortex.
34516313	5	25	theme	brain	1304:1308	arg1	region					1310:1315	this brain region	1299:1315	this brain region	1299:1315	Fucogangliosides and species with the glycan chain elongated by either O-acetylation and/or acetate anion attachments were also detected; the later modification was for the first time discovered in this brain region.
34516313	2	26	theme	negative	430:437	arg1	mode					443:446	the negative ion mode	426:446	the negative ion mode	426:446	The experiments, conducted on a nanoelectrospray Orbitrap mass spectroscope in the negative ion mode, allowed the discrimination in the native mixture extracted from human motor cortex of no less than 83 different gangliosides, which represents the highest number of structures identified so far in this brain region.
34516313	6	27	theme	hepta	1365:1369	arg1	identification					1347:1360	the identification	1343:1360	the identification of hepta and octasialylated species of GS1 and GO1 type, which are among the structures with the longest oligosaccharide chain discovered so far in the human brain	1343:1524	Of major significance is the identification of hepta and octasialylated species of GS1 and GO1 type, which are among the structures with the longest oligosaccharide chain discovered so far in the human brain.
34516313	3	28	theme	higher	912:917	arg1	degree					919:924	a much higher degree	905:924	a much higher degree of sialylation than previously known	905:961	The spectral data, acquired in high-resolution mass spectrometry mode with a remarkable sensitivity and an average mass accuracy of 4.48 ppm, also show that the gangliosidome of motor cortex is generally characterized by species exhibiting a much higher degree of sialylation than previously known.
34516313	1	29	theme	human	326:330	arg1	cortex					338:343	the human motor cortex	322:343	the human motor cortex	322:343	We have developed here a superior methodology based on high-resolution mass spectrometry for screening and fragmentation analysis of gangliosides extracted and purified from the human motor cortex .
34516313	0	30	theme	novel	76:80	arg1	structures					97:106	novel polysialylated structures	76:106	novel polysialylated structures	76:106	High-resolution mass spectrometry reveals a complex ganglioside pattern and novel polysialylated structures associated with the human motor cortex.
34516313	0	31	theme	High-resolution	0:14	arg1	spectrometry					21:32	High-resolution mass spectrometry	0:32	High-resolution mass spectrometry	0:32	High-resolution mass spectrometry reveals a complex ganglioside pattern and novel polysialylated structures associated with the human motor cortex.
34516313	4	32	theme	long	1060:1063	arg1	chains					1076:1081	long saccharide chains	1060:1081	long saccharide chains	1060:1081	Motor cortex was found dominated by complex structures with a sialylation degree ≥3, exhibiting long saccharide chains, in the G1 class.
34516313	7	33	theme	last	1534:1537	arg1	stage					1539:1543	the last stage	1530:1543	the last stage of research	1530:1555	In the last stage of research, tandem mass spectrometry performed by higher energy collision dissociation provided structural data documenting the occurrence of GT1b (d18:1/20:0) isomer in the human motor cortex.
34516313	3	34	theme	spectral	669:676	arg1	data					678:681	The spectral data	665:681	The spectral data	665:681	The spectral data, acquired in high-resolution mass spectrometry mode with a remarkable sensitivity and an average mass accuracy of 4.48 ppm, also show that the gangliosidome of motor cortex is generally characterized by species exhibiting a much higher degree of sialylation than previously known.
34516313	1	35	theme	motor	332:336	arg1	cortex					338:343	the human motor cortex	322:343	the human motor cortex	322:343	We have developed here a superior methodology based on high-resolution mass spectrometry for screening and fragmentation analysis of gangliosides extracted and purified from the human motor cortex .
34516313	4	36	with	structures	1008:1017	arg1	≥3					1045:1046	a sialylation degree ≥3	1024:1046	a sialylation degree ≥3	1024:1046	Motor cortex was found dominated by complex structures with a sialylation degree ≥3, exhibiting long saccharide chains, in the G1 class.
34516313	4	37	theme	degree	1038:1043	arg1	≥3					1045:1046	a sialylation degree ≥3	1024:1046	a sialylation degree ≥3	1024:1046	Motor cortex was found dominated by complex structures with a sialylation degree ≥3, exhibiting long saccharide chains, in the G1 class.
34516313	6	38	theme	type	1413:1416	arg1	hepta					1365:1369	hepta	1365:1369	hepta	1365:1369	Of major significance is the identification of hepta and octasialylated species of GS1 and GO1 type, which are among the structures with the longest oligosaccharide chain discovered so far in the human brain.
34516313	6	38	theme	type	1413:1416	arg1	species					1390:1396	octasialylated species	1375:1396	octasialylated species	1375:1396	Of major significance is the identification of hepta and octasialylated species of GS1 and GO1 type, which are among the structures with the longest oligosaccharide chain discovered so far in the human brain.
34516313	4	39	theme	G1	1091:1092	arg1	class					1094:1098	the G1 class	1087:1098	the G1 class	1087:1098	Motor cortex was found dominated by complex structures with a sialylation degree ≥3, exhibiting long saccharide chains, in the G1 class.
34516313	5	40	theme	later	1243:1247	arg1	modification					1249:1260	the later modification	1239:1260	the later modification	1239:1260	Fucogangliosides and species with the glycan chain elongated by either O-acetylation and/or acetate anion attachments were also detected; the later modification was for the first time discovered in this brain region.
34516313	2	41	theme	mass	405:408	arg1	spectroscope					410:421	a nanoelectrospray Orbitrap mass spectroscope	377:421	a nanoelectrospray Orbitrap mass spectroscope in the negative ion mode	377:446	The experiments, conducted on a nanoelectrospray Orbitrap mass spectroscope in the negative ion mode, allowed the discrimination in the native mixture extracted from human motor cortex of no less than 83 different gangliosides, which represents the highest number of structures identified so far in this brain region.
34516313	1	42	theme	fragmentation	255:267	arg1	analysis					269:276	fragmentation analysis	255:276	fragmentation analysis	255:276	We have developed here a superior methodology based on high-resolution mass spectrometry for screening and fragmentation analysis of gangliosides extracted and purified from the human motor cortex .
34516313	2	43	theme	nanoelectrospray	379:394	arg1	spectroscope					410:421	a nanoelectrospray Orbitrap mass spectroscope	377:421	a nanoelectrospray Orbitrap mass spectroscope in the negative ion mode	377:446	The experiments, conducted on a nanoelectrospray Orbitrap mass spectroscope in the negative ion mode, allowed the discrimination in the native mixture extracted from human motor cortex of no less than 83 different gangliosides, which represents the highest number of structures identified so far in this brain region.
34516313	6	44	theme	oligosaccharide	1467:1481	arg1	chain					1483:1487	the longest oligosaccharide chain	1455:1487	the longest oligosaccharide chain discovered so far in the human brain	1455:1524	Of major significance is the identification of hepta and octasialylated species of GS1 and GO1 type, which are among the structures with the longest oligosaccharide chain discovered so far in the human brain.
34516313	3	45	theme	mass	712:715	arg1	spectrometry					717:728	high-resolution mass spectrometry	696:728	high-resolution mass spectrometry mode	696:733	The spectral data, acquired in high-resolution mass spectrometry mode with a remarkable sensitivity and an average mass accuracy of 4.48 ppm, also show that the gangliosidome of motor cortex is generally characterized by species exhibiting a much higher degree of sialylation than previously known.
34516313	1	46	theme	superior	173:180	arg1	methodology					182:192	here a superior methodology	166:192	here a superior methodology based on high-resolution mass spectrometry for screening and fragmentation analysis of gangliosides extracted and purified from the human motor cortex	166:343	We have developed here a superior methodology based on high-resolution mass spectrometry for screening and fragmentation analysis of gangliosides extracted and purified from the human motor cortex .
34516313	2	47	theme	native	483:488	arg1	mixture					490:496	the native mixture	479:496	the native mixture extracted from human motor cortex of no less than 83 different gangliosides, which represents the highest number of structures identified so far in this brain region	479:662	The experiments, conducted on a nanoelectrospray Orbitrap mass spectroscope in the negative ion mode, allowed the discrimination in the native mixture extracted from human motor cortex of no less than 83 different gangliosides, which represents the highest number of structures identified so far in this brain region.
34516313	2	48	theme	human	513:517	arg1	cortex					525:530	human motor cortex	513:530	human motor cortex of no less than 83 different gangliosides, which represents the highest number of structures identified so far in this brain region	513:662	The experiments, conducted on a nanoelectrospray Orbitrap mass spectroscope in the negative ion mode, allowed the discrimination in the native mixture extracted from human motor cortex of no less than 83 different gangliosides, which represents the highest number of structures identified so far in this brain region.
34516313	0	49	theme	ganglioside	52:62	arg1	pattern					64:70	a complex ganglioside pattern	42:70	a complex ganglioside pattern	42:70	High-resolution mass spectrometry reveals a complex ganglioside pattern and novel polysialylated structures associated with the human motor cortex.
34516313	3	50	theme	mass	780:783	arg1	accuracy					785:792	an average mass accuracy	769:792	an average mass accuracy	769:792	The spectral data, acquired in high-resolution mass spectrometry mode with a remarkable sensitivity and an average mass accuracy of 4.48 ppm, also show that the gangliosidome of motor cortex is generally characterized by species exhibiting a much higher degree of sialylation than previously known.
34516313	4	51	dep	found	981:985	arg1	dominated					987:995	dominated	987:995	dominated by complex structures with a sialylation degree ≥3, exhibiting long saccharide chains, in the G1 class	987:1098	Motor cortex was found dominated by complex structures with a sialylation degree ≥3, exhibiting long saccharide chains, in the G1 class.
34516313	1	52	theme	high-resolution	203:217	arg1	spectrometry					224:235	high-resolution mass spectrometry	203:235	high-resolution mass spectrometry for screening and fragmentation analysis of gangliosides extracted and purified from the human motor cortex	203:343	We have developed here a superior methodology based on high-resolution mass spectrometry for screening and fragmentation analysis of gangliosides extracted and purified from the human motor cortex .
34516313	7	53	theme	collision	1610:1618	arg1	dissociation					1620:1631	higher energy collision dissociation	1596:1631	higher energy collision dissociation	1596:1631	In the last stage of research, tandem mass spectrometry performed by higher energy collision dissociation provided structural data documenting the occurrence of GT1b (d18:1/20:0) isomer in the human motor cortex.
34516313	2	54	theme	different	551:559	arg1	gangliosides					561:572	no less than 83 different gangliosides	535:572	no less than 83 different gangliosides	535:572	The experiments, conducted on a nanoelectrospray Orbitrap mass spectroscope in the negative ion mode, allowed the discrimination in the native mixture extracted from human motor cortex of no less than 83 different gangliosides, which represents the highest number of structures identified so far in this brain region.
34516313	7	55	theme	isomer	1706:1711	arg1	occurrence					1674:1683	the occurrence	1670:1683	the occurrence of GT1b (d18:1/20:0) isomer in the human motor cortex	1670:1737	In the last stage of research, tandem mass spectrometry performed by higher energy collision dissociation provided structural data documenting the occurrence of GT1b (d18:1/20:0) isomer in the human motor cortex.
34516313	6	56	theme	significance	1327:1338	arg1	identification					1347:1360	the identification	1343:1360	the identification of hepta and octasialylated species of GS1 and GO1 type, which are among the structures with the longest oligosaccharide chain discovered so far in the human brain	1343:1524	Of major significance is the identification of hepta and octasialylated species of GS1 and GO1 type, which are among the structures with the longest oligosaccharide chain discovered so far in the human brain.
34516313	7	57	theme	higher	1596:1601	arg1	dissociation					1620:1631	higher energy collision dissociation	1596:1631	higher energy collision dissociation	1596:1631	In the last stage of research, tandem mass spectrometry performed by higher energy collision dissociation provided structural data documenting the occurrence of GT1b (d18:1/20:0) isomer in the human motor cortex.
34516313	2	58	theme	highest	596:602	arg1	number					604:609	the highest number	592:609	the highest number of structures identified so far in this brain region	592:662	The experiments, conducted on a nanoelectrospray Orbitrap mass spectroscope in the negative ion mode, allowed the discrimination in the native mixture extracted from human motor cortex of no less than 83 different gangliosides, which represents the highest number of structures identified so far in this brain region.
34516313	5	59	with	species	1122:1128	arg1	chain					1146:1150	the glycan chain	1135:1150	the glycan chain elongated by either O-acetylation and/or acetate anion attachments	1135:1217	Fucogangliosides and species with the glycan chain elongated by either O-acetylation and/or acetate anion attachments were also detected; the later modification was for the first time discovered in this brain region.
34516313	5	60	with	Fucogangliosides	1101:1116	arg1	chain					1146:1150	the glycan chain	1135:1150	the glycan chain elongated by either O-acetylation and/or acetate anion attachments	1135:1217	Fucogangliosides and species with the glycan chain elongated by either O-acetylation and/or acetate anion attachments were also detected; the later modification was for the first time discovered in this brain region.
34516313	6	61	theme	GO1	1409:1411	arg1	type					1413:1416	GO1 type	1409:1416	GO1 type	1409:1416	Of major significance is the identification of hepta and octasialylated species of GS1 and GO1 type, which are among the structures with the longest oligosaccharide chain discovered so far in the human brain.
34516313	7	62	theme	mass	1565:1568	arg1	spectrometry					1570:1581	tandem mass spectrometry	1558:1581	tandem mass spectrometry performed by higher energy collision dissociation	1558:1631	In the last stage of research, tandem mass spectrometry performed by higher energy collision dissociation provided structural data documenting the occurrence of GT1b (d18:1/20:0) isomer in the human motor cortex.
34516313	6	63	theme	GS1	1401:1403	arg1	hepta					1365:1369	hepta	1365:1369	hepta	1365:1369	Of major significance is the identification of hepta and octasialylated species of GS1 and GO1 type, which are among the structures with the longest oligosaccharide chain discovered so far in the human brain.
34516313	6	63	theme	GS1	1401:1403	arg1	species					1390:1396	octasialylated species	1375:1396	octasialylated species	1375:1396	Of major significance is the identification of hepta and octasialylated species of GS1 and GO1 type, which are among the structures with the longest oligosaccharide chain discovered so far in the human brain.
34516313	5	64	theme	glycan	1139:1144	arg1	chain					1146:1150	the glycan chain	1135:1150	the glycan chain elongated by either O-acetylation and/or acetate anion attachments	1135:1217	Fucogangliosides and species with the glycan chain elongated by either O-acetylation and/or acetate anion attachments were also detected; the later modification was for the first time discovered in this brain region.
34516313	7	65	theme	motor	1726:1730	arg1	cortex					1732:1737	the human motor cortex	1716:1737	the human motor cortex	1716:1737	In the last stage of research, tandem mass spectrometry performed by higher energy collision dissociation provided structural data documenting the occurrence of GT1b (d18:1/20:0) isomer in the human motor cortex.
34516313	4	66	theme	complex	1000:1006	arg1	structures					1008:1017	complex structures	1000:1017	complex structures with a sialylation degree ≥3, exhibiting long saccharide chains, in the G1 class	1000:1098	Motor cortex was found dominated by complex structures with a sialylation degree ≥3, exhibiting long saccharide chains, in the G1 class.
34516313	3	67	theme	cortex	849:854	arg1	gangliosidome					826:838	the gangliosidome	822:838	the gangliosidome of motor cortex	822:854	The spectral data, acquired in high-resolution mass spectrometry mode with a remarkable sensitivity and an average mass accuracy of 4.48 ppm, also show that the gangliosidome of motor cortex is generally characterized by species exhibiting a much higher degree of sialylation than previously known.
34516313	6	68	theme	species	1390:1396	arg1	identification					1347:1360	the identification	1343:1360	the identification of hepta and octasialylated species of GS1 and GO1 type, which are among the structures with the longest oligosaccharide chain discovered so far in the human brain	1343:1524	Of major significance is the identification of hepta and octasialylated species of GS1 and GO1 type, which are among the structures with the longest oligosaccharide chain discovered so far in the human brain.
34516313	4	69	theme	Motor	964:968	arg1	cortex					970:975	Motor cortex	964:975	Motor cortex	964:975	Motor cortex was found dominated by complex structures with a sialylation degree ≥3, exhibiting long saccharide chains, in the G1 class.
34516313	5	70	dep	O-acetylation	1172:1184	arg1	attachments					1207:1217	anion attachments	1201:1217	anion attachments	1201:1217	Fucogangliosides and species with the glycan chain elongated by either O-acetylation and/or acetate anion attachments were also detected; the later modification was for the first time discovered in this brain region.
34516313	0	71	theme	human	128:132	arg1	cortex					140:145	the human motor cortex	124:145	the human motor cortex	124:145	High-resolution mass spectrometry reveals a complex ganglioside pattern and novel polysialylated structures associated with the human motor cortex.
34516313	6	72	with	structures	1439:1448	arg1	chain					1483:1487	the longest oligosaccharide chain	1455:1487	the longest oligosaccharide chain discovered so far in the human brain	1455:1524	Of major significance is the identification of hepta and octasialylated species of GS1 and GO1 type, which are among the structures with the longest oligosaccharide chain discovered so far in the human brain.
34516313	0	73	gly	polysialylated	82:95	arg1	structures					97:106	novel polysialylated structures	76:106	novel polysialylated structures	76:106	High-resolution mass spectrometry reveals a complex ganglioside pattern and novel polysialylated structures associated with the human motor cortex.
34516313	3	74	theme	sialylation	929:939	arg1	degree					919:924	a much higher degree	905:924	a much higher degree of sialylation than previously known	905:961	The spectral data, acquired in high-resolution mass spectrometry mode with a remarkable sensitivity and an average mass accuracy of 4.48 ppm, also show that the gangliosidome of motor cortex is generally characterized by species exhibiting a much higher degree of sialylation than previously known.
34516313	0	75	theme	mass	16:19	arg1	spectrometry					21:32	High-resolution mass spectrometry	0:32	High-resolution mass spectrometry	0:32	High-resolution mass spectrometry reveals a complex ganglioside pattern and novel polysialylated structures associated with the human motor cortex.
34516313	7	76	theme	d18:1/20:0	1694:1703	arg1	isomer					1706:1711	GT1b (d18:1/20:0) isomer	1688:1711	GT1b (d18:1/20:0) isomer	1688:1711	In the last stage of research, tandem mass spectrometry performed by higher energy collision dissociation provided structural data documenting the occurrence of GT1b (d18:1/20:0) isomer in the human motor cortex.
34516313	5	77	theme	anion	1201:1205	arg1	attachments					1207:1217	anion attachments	1201:1217	anion attachments	1201:1217	Fucogangliosides and species with the glycan chain elongated by either O-acetylation and/or acetate anion attachments were also detected; the later modification was for the first time discovered in this brain region.
34516313	7	78	theme	GT1b	1688:1691	arg1	isomer					1706:1711	GT1b (d18:1/20:0) isomer	1688:1711	GT1b (d18:1/20:0) isomer	1688:1711	In the last stage of research, tandem mass spectrometry performed by higher energy collision dissociation provided structural data documenting the occurrence of GT1b (d18:1/20:0) isomer in the human motor cortex.
34516313	0	79	theme	complex	44:50	arg1	pattern					64:70	a complex ganglioside pattern	42:70	a complex ganglioside pattern	42:70	High-resolution mass spectrometry reveals a complex ganglioside pattern and novel polysialylated structures associated with the human motor cortex.
34516313	4	80	from	≥3	1045:1046	arg1	class					1094:1098	the G1 class	1087:1098	the G1 class	1087:1098	Motor cortex was found dominated by complex structures with a sialylation degree ≥3, exhibiting long saccharide chains, in the G1 class.
34516313	6	81	gly	octasialylated	1375:1388	arg1	species					1390:1396	octasialylated species	1375:1396	octasialylated species	1375:1396	Of major significance is the identification of hepta and octasialylated species of GS1 and GO1 type, which are among the structures with the longest oligosaccharide chain discovered so far in the human brain.
34516313	2	82	theme	ion	439:441	arg1	mode					443:446	the negative ion mode	426:446	the negative ion mode	426:446	The experiments, conducted on a nanoelectrospray Orbitrap mass spectroscope in the negative ion mode, allowed the discrimination in the native mixture extracted from human motor cortex of no less than 83 different gangliosides, which represents the highest number of structures identified so far in this brain region.
34516313	2	83	from	spectroscope	410:421	arg1	mode					443:446	the negative ion mode	426:446	the negative ion mode	426:446	The experiments, conducted on a nanoelectrospray Orbitrap mass spectroscope in the negative ion mode, allowed the discrimination in the native mixture extracted from human motor cortex of no less than 83 different gangliosides, which represents the highest number of structures identified so far in this brain region.
34516313	4	84	theme	sialylation	1026:1036	arg1	≥3					1045:1046	a sialylation degree ≥3	1024:1046	a sialylation degree ≥3	1024:1046	Motor cortex was found dominated by complex structures with a sialylation degree ≥3, exhibiting long saccharide chains, in the G1 class.
34516313	3	85	dep	sensitivity	753:763	arg1	a					740:740	a	740:740	a	740:740	The spectral data, acquired in high-resolution mass spectrometry mode with a remarkable sensitivity and an average mass accuracy of 4.48 ppm, also show that the gangliosidome of motor cortex is generally characterized by species exhibiting a much higher degree of sialylation than previously known.
34516313	7	86	from	occurrence	1674:1683	arg1	cortex					1732:1737	the human motor cortex	1716:1737	the human motor cortex	1716:1737	In the last stage of research, tandem mass spectrometry performed by higher energy collision dissociation provided structural data documenting the occurrence of GT1b (d18:1/20:0) isomer in the human motor cortex.
34516313	3	87	theme	spectrometry	717:728	arg1	mode					730:733	high-resolution mass spectrometry mode	696:733	high-resolution mass spectrometry mode	696:733	The spectral data, acquired in high-resolution mass spectrometry mode with a remarkable sensitivity and an average mass accuracy of 4.48 ppm, also show that the gangliosidome of motor cortex is generally characterized by species exhibiting a much higher degree of sialylation than previously known.
34516313	2	88	theme	Orbitrap	396:403	arg1	spectroscope					410:421	a nanoelectrospray Orbitrap mass spectroscope	377:421	a nanoelectrospray Orbitrap mass spectroscope in the negative ion mode	377:446	The experiments, conducted on a nanoelectrospray Orbitrap mass spectroscope in the negative ion mode, allowed the discrimination in the native mixture extracted from human motor cortex of no less than 83 different gangliosides, which represents the highest number of structures identified so far in this brain region.
32365492	7	0	theme	mutant	1205:1210	arg1	cells					1212:1216	The mutant cells	1201:1216	The mutant cells	1201:1216	The mutant cells also showed reduced ability to stimulate cytokine production and phagocytosis by human mononuclear cells and macrophages, respectively.
32365492	8	1	theme	innate	1497:1502	arg1	cells					1511:1515	innate immune cells	1497:1515	innate immune cells	1497:1515	Collectively, these data showed that loss of Kex2 affected the cell wall composition, the protein glycosylation pathways, and interaction with innate immune cells.
32365492	2	2	theme	Kex2	367:370	arg1	protease					372:379	The Golgi-resident Kex2 protease	348:379	The Golgi-resident Kex2 protease	348:379	The Golgi-resident Kex2 protease is involved in such processing and disruption of its encoding gene affected virulence and dimorphism.
32365492	2	3	theme	Golgi-resident	352:365	arg1	protease					372:379	The Golgi-resident Kex2 protease	348:379	The Golgi-resident Kex2 protease	348:379	The Golgi-resident Kex2 protease is involved in such processing and disruption of its encoding gene affected virulence and dimorphism.
32365492	1	4	theme	Golgi	236:240	arg1	complex					242:248	the Golgi complex	232:248	the Golgi complex	232:248	The secretory pathway in Candida albicans involves the protein translocation into the lumen of the endoplasmic reticulum and transport to the Golgi complex, where proteins undergo posttranslational modifications, including glycosylation and proteolysis.
32365492	0	5	theme	Immune	80:85	arg1	Cells					87:91	Innate Immune Cells	73:91	Innate Immune Cells	73:91	Loss of Kex2 Affects the Candida albicans Cell Wall and Interaction with Innate Immune Cells.
32365492	0	6	with	Interaction	56:66	arg1	Cells					87:91	Innate Immune Cells	73:91	Innate Immune Cells	73:91	Loss of Kex2 Affects the Candida albicans Cell Wall and Interaction with Innate Immune Cells.
32365492	0	7	theme	Innate	73:78	arg1	Cells					87:91	Innate Immune Cells	73:91	Innate Immune Cells	73:91	Loss of Kex2 Affects the Candida albicans Cell Wall and Interaction with Innate Immune Cells.
32365492	6	8	theme	α-1,6-mannosyltransferase	1120:1144	arg1	activities					1146:1155	the α-1,2-mannosyltransferase and α-1,6-mannosyltransferase activities	1086:1155	the α-1,2-mannosyltransferase and α-1,6-mannosyltransferase activities	1086:1155	The defects in the mannan content are likely linked to changes in Golgi-resident enzymes, as the α-1,2-mannosyltransferase and α-1,6-mannosyltransferase activities were incremented and reduced, respectively.
32365492	7	9	theme	reduced	1230:1236	arg1	ability					1238:1244	reduced ability	1230:1244	reduced ability to stimulate cytokine production	1230:1277	The mutant cells also showed reduced ability to stimulate cytokine production and phagocytosis by human mononuclear cells and macrophages, respectively.
32365492	5	10	from	chitin	970:975	arg1	levels					942:947	the levels	938:947	the levels of O-linked mannans	938:967	The characterization of this strain showed defects in the cell wall composition, with a reduction in the N-linked mannan content, and the increment in the levels of O-linked mannans, chitin, and β-glucans.
32365492	5	11	theme	N-linked	892:899	arg1	mannan					901:906	the N-linked mannan content, and the increment in the levels of O-linked mannans, chitin, and β-glucans	888:990	mannan	901:906	The characterization of this strain showed defects in the cell wall composition, with a reduction in the N-linked mannan content, and the increment in the levels of O-linked mannans, chitin, and β-glucans.
32365492	0	12	with	Wall	47:50	arg1	Cells					87:91	Innate Immune Cells	73:91	Innate Immune Cells	73:91	Loss of Kex2 Affects the Candida albicans Cell Wall and Interaction with Innate Immune Cells.
32365492	4	13	theme	locus	775:779	arg1	RPS1					781:784	the neutral locus RPS1	763:784	the neutral locus RPS1	763:784	Since these conditions are known to affect the cellular fitness and the host-fungus interaction, here we generated a kex2Δ null mutant strain with URA3 placed into the neutral locus RPS1.
32365492	1	14	theme	protein	149:155	arg1	translocation					157:169	the protein translocation	145:169	the protein translocation	145:169	The secretory pathway in Candida albicans involves the protein translocation into the lumen of the endoplasmic reticulum and transport to the Golgi complex, where proteins undergo posttranslational modifications, including glycosylation and proteolysis.
32365492	5	15	dep	mannan	901:906	arg1	content					908:914	content	908:914	content	908:914	The characterization of this strain showed defects in the cell wall composition, with a reduction in the N-linked mannan content, and the increment in the levels of O-linked mannans, chitin, and β-glucans.
32365492	1	16	from	pathway	108:114	arg1	albicans					127:134	Candida albicans	119:134	Candida albicans	119:134	The secretory pathway in Candida albicans involves the protein translocation into the lumen of the endoplasmic reticulum and transport to the Golgi complex, where proteins undergo posttranslational modifications, including glycosylation and proteolysis.
32365492	4	17	theme	neutral	767:773	arg1	RPS1					781:784	the neutral locus RPS1	763:784	the neutral locus RPS1	763:784	Since these conditions are known to affect the cellular fitness and the host-fungus interaction, here we generated a kex2Δ null mutant strain with URA3 placed into the neutral locus RPS1.
32365492	2	18	theme	such	396:399	arg1	processing					401:410	such processing	396:410	such processing	396:410	The Golgi-resident Kex2 protease is involved in such processing and disruption of its encoding gene affected virulence and dimorphism.
32365492	6	19	from	defects	997:1003	arg1	content					1019:1025	the mannan content	1008:1025	the mannan content	1008:1025	The defects in the mannan content are likely linked to changes in Golgi-resident enzymes, as the α-1,2-mannosyltransferase and α-1,6-mannosyltransferase activities were incremented and reduced, respectively.
32365492	6	20	attach	linked	1038:1043	arg2	defects					997:1003	The defects	993:1003	The defects in the mannan content	993:1025	The defects in the mannan content are likely linked to changes in Golgi-resident enzymes, as the α-1,2-mannosyltransferase and α-1,6-mannosyltransferase activities were incremented and reduced, respectively.
32365492	6	20	attach	linked	1038:1043	arg1	changes					1048:1054	changes	1048:1054	changes in Golgi-resident enzymes	1048:1080	The defects in the mannan content are likely linked to changes in Golgi-resident enzymes, as the α-1,2-mannosyltransferase and α-1,6-mannosyltransferase activities were incremented and reduced, respectively.
32365492	3	21	theme	KEX2	587:590	arg1	locus					592:596	the KEX2 locus	583:596	the KEX2 locus	583:596	These previous studies were performed using cells without URA3 or with URA3 ectopically placed into the KEX2 locus.
32365492	7	22	theme	human	1299:1303	arg1	cells					1317:1321	human mononuclear cells	1299:1321	human mononuclear cells	1299:1321	The mutant cells also showed reduced ability to stimulate cytokine production and phagocytosis by human mononuclear cells and macrophages, respectively.
32365492	4	23	theme	host-fungus	671:681	arg1	interaction					683:693	the host-fungus interaction	667:693	the host-fungus interaction	667:693	Since these conditions are known to affect the cellular fitness and the host-fungus interaction, here we generated a kex2Δ null mutant strain with URA3 placed into the neutral locus RPS1.
32365492	2	24	theme	gene	443:446	arg1	disruption					416:425	disruption	416:425	disruption of its encoding gene	416:446	The Golgi-resident Kex2 protease is involved in such processing and disruption of its encoding gene affected virulence and dimorphism.
32365492	5	25	from	reduction	875:883	arg1	chitin					970:975	chitin	970:975	chitin	970:975	The characterization of this strain showed defects in the cell wall composition, with a reduction in the N-linked mannan content, and the increment in the levels of O-linked mannans, chitin, and β-glucans.
32365492	5	25	from	reduction	875:883	arg1	mannan					901:906	the N-linked mannan content, and the increment in the levels of O-linked mannans, chitin, and β-glucans	888:990	mannan	901:906	The characterization of this strain showed defects in the cell wall composition, with a reduction in the N-linked mannan content, and the increment in the levels of O-linked mannans, chitin, and β-glucans.
32365492	5	25	from	reduction	875:883	arg1	increment					925:933	the increment	921:933	the increment in the levels of O-linked mannans	921:967	The characterization of this strain showed defects in the cell wall composition, with a reduction in the N-linked mannan content, and the increment in the levels of O-linked mannans, chitin, and β-glucans.
32365492	5	25	from	reduction	875:883	arg1	β-glucans					982:990	β-glucans	982:990	β-glucans	982:990	The characterization of this strain showed defects in the cell wall composition, with a reduction in the N-linked mannan content, and the increment in the levels of O-linked mannans, chitin, and β-glucans.
32365492	5	26	from	mannan	901:906	arg1	levels					942:947	the levels	938:947	the levels of O-linked mannans	938:967	The characterization of this strain showed defects in the cell wall composition, with a reduction in the N-linked mannan content, and the increment in the levels of O-linked mannans, chitin, and β-glucans.
32365492	6	27	theme	mannan	1012:1017	arg1	content					1019:1025	the mannan content	1008:1025	the mannan content	1008:1025	The defects in the mannan content are likely linked to changes in Golgi-resident enzymes, as the α-1,2-mannosyltransferase and α-1,6-mannosyltransferase activities were incremented and reduced, respectively.
32365492	2	28	theme	encoding	434:441	arg1	gene					443:446	its encoding gene	430:446	its encoding gene	430:446	The Golgi-resident Kex2 protease is involved in such processing and disruption of its encoding gene affected virulence and dimorphism.
32365492	8	29	theme	protein	1444:1450	arg1	pathways					1466:1473	the protein glycosylation pathways	1440:1473	the protein glycosylation pathways	1440:1473	Collectively, these data showed that loss of Kex2 affected the cell wall composition, the protein glycosylation pathways, and interaction with innate immune cells.
32365492	6	30	theme	α-1,2-mannosyltransferase	1090:1114	arg1	activities					1146:1155	the α-1,2-mannosyltransferase and α-1,6-mannosyltransferase activities	1086:1155	the α-1,2-mannosyltransferase and α-1,6-mannosyltransferase activities	1086:1155	The defects in the mannan content are likely linked to changes in Golgi-resident enzymes, as the α-1,2-mannosyltransferase and α-1,6-mannosyltransferase activities were incremented and reduced, respectively.
32365492	6	31	theme	Golgi-resident	1059:1072	arg1	enzymes					1074:1080	Golgi-resident enzymes	1059:1080	Golgi-resident enzymes	1059:1080	The defects in the mannan content are likely linked to changes in Golgi-resident enzymes, as the α-1,2-mannosyltransferase and α-1,6-mannosyltransferase activities were incremented and reduced, respectively.
32365492	0	32	theme	Candida	25:31	arg1	albicans					33:40	Candida albicans	25:40	the Candida albicans Cell Wall	21:50	Loss of Kex2 Affects the Candida albicans Cell Wall and Interaction with Innate Immune Cells.
32365492	1	33	theme	posttranslational	274:290	arg1	proteolysis					335:345	proteolysis	335:345	proteolysis	335:345	The secretory pathway in Candida albicans involves the protein translocation into the lumen of the endoplasmic reticulum and transport to the Golgi complex, where proteins undergo posttranslational modifications, including glycosylation and proteolysis.
32365492	1	33	theme	posttranslational	274:290	arg1	modifications					292:304	posttranslational modifications	274:304	posttranslational modifications	274:304	The secretory pathway in Candida albicans involves the protein translocation into the lumen of the endoplasmic reticulum and transport to the Golgi complex, where proteins undergo posttranslational modifications, including glycosylation and proteolysis.
32365492	1	33	theme	posttranslational	274:290	arg1	glycosylation					317:329	glycosylation	317:329	glycosylation	317:329	The secretory pathway in Candida albicans involves the protein translocation into the lumen of the endoplasmic reticulum and transport to the Golgi complex, where proteins undergo posttranslational modifications, including glycosylation and proteolysis.
32365492	8	34	theme	Kex2	1399:1402	arg1	loss					1391:1394	loss	1391:1394	loss of Kex2	1391:1402	Collectively, these data showed that loss of Kex2 affected the cell wall composition, the protein glycosylation pathways, and interaction with innate immune cells.
32365492	5	35	from	β-glucans	982:990	arg1	levels					942:947	the levels	938:947	the levels of O-linked mannans	938:967	The characterization of this strain showed defects in the cell wall composition, with a reduction in the N-linked mannan content, and the increment in the levels of O-linked mannans, chitin, and β-glucans.
32365492	7	36	dep	ability	1238:1244	arg1	stimulate					1249:1257	stimulate	1249:1257	to stimulate cytokine production	1246:1277	The mutant cells also showed reduced ability to stimulate cytokine production and phagocytosis by human mononuclear cells and macrophages, respectively.
32365492	5	37	theme	wall	850:853	arg1	composition					855:865	the cell wall composition	841:865	the cell wall composition	841:865	The characterization of this strain showed defects in the cell wall composition, with a reduction in the N-linked mannan content, and the increment in the levels of O-linked mannans, chitin, and β-glucans.
32365492	5	38	theme	strain	816:821	arg1	characterization					791:806	The characterization	787:806	The characterization of this strain	787:821	The characterization of this strain showed defects in the cell wall composition, with a reduction in the N-linked mannan content, and the increment in the levels of O-linked mannans, chitin, and β-glucans.
32365492	5	39	theme	cell	845:848	arg1	composition					855:865	the cell wall composition	841:865	the cell wall composition	841:865	The characterization of this strain showed defects in the cell wall composition, with a reduction in the N-linked mannan content, and the increment in the levels of O-linked mannans, chitin, and β-glucans.
32365492	1	40	theme	endoplasmic	193:203	arg1	reticulum					205:213	the endoplasmic reticulum	189:213	the endoplasmic reticulum	189:213	The secretory pathway in Candida albicans involves the protein translocation into the lumen of the endoplasmic reticulum and transport to the Golgi complex, where proteins undergo posttranslational modifications, including glycosylation and proteolysis.
32365492	0	41	theme	Cell	42:45	arg1	Wall					47:50	the Candida albicans Cell Wall	21:50	the Candida albicans Cell Wall	21:50	Loss of Kex2 Affects the Candida albicans Cell Wall and Interaction with Innate Immune Cells.
32365492	8	42	with	composition	1427:1437	arg1	cells					1511:1515	innate immune cells	1497:1515	innate immune cells	1497:1515	Collectively, these data showed that loss of Kex2 affected the cell wall composition, the protein glycosylation pathways, and interaction with innate immune cells.
32365492	8	43	theme	glycosylation	1452:1464	arg1	pathways					1466:1473	the protein glycosylation pathways	1440:1473	the protein glycosylation pathways	1440:1473	Collectively, these data showed that loss of Kex2 affected the cell wall composition, the protein glycosylation pathways, and interaction with innate immune cells.
32365492	8	44	with	pathways	1466:1473	arg1	cells					1511:1515	innate immune cells	1497:1515	innate immune cells	1497:1515	Collectively, these data showed that loss of Kex2 affected the cell wall composition, the protein glycosylation pathways, and interaction with innate immune cells.
32365492	4	45	theme	kex2Δ	716:720	arg1	strain					734:739	a kex2Δ null mutant strain	714:739	a kex2Δ null mutant strain with URA3 placed into the neutral locus RPS1	714:784	Since these conditions are known to affect the cellular fitness and the host-fungus interaction, here we generated a kex2Δ null mutant strain with URA3 placed into the neutral locus RPS1.
32365492	0	46	theme	albicans	33:40	arg1	Wall					47:50	the Candida albicans Cell Wall	21:50	the Candida albicans Cell Wall	21:50	Loss of Kex2 Affects the Candida albicans Cell Wall and Interaction with Innate Immune Cells.
32365492	5	47	from	defects	830:836	arg1	composition					855:865	the cell wall composition	841:865	the cell wall composition	841:865	The characterization of this strain showed defects in the cell wall composition, with a reduction in the N-linked mannan content, and the increment in the levels of O-linked mannans, chitin, and β-glucans.
32365492	1	48	theme	secretory	98:106	arg1	pathway					108:114	The secretory pathway	94:114	The secretory pathway in Candida albicans	94:134	The secretory pathway in Candida albicans involves the protein translocation into the lumen of the endoplasmic reticulum and transport to the Golgi complex, where proteins undergo posttranslational modifications, including glycosylation and proteolysis.
32365492	5	49	from	increment	925:933	arg1	levels					942:947	the levels	938:947	the levels of O-linked mannans	938:967	The characterization of this strain showed defects in the cell wall composition, with a reduction in the N-linked mannan content, and the increment in the levels of O-linked mannans, chitin, and β-glucans.
32365492	4	50	with	strain	734:739	arg1	URA3					746:749	URA3	746:749	URA3 placed into the neutral locus RPS1	746:784	Since these conditions are known to affect the cellular fitness and the host-fungus interaction, here we generated a kex2Δ null mutant strain with URA3 placed into the neutral locus RPS1.
32365492	5	51	link	N-linked	892:899	arg1	mannan					901:906	the N-linked mannan content, and the increment in the levels of O-linked mannans, chitin, and β-glucans	888:990	mannan	901:906	The characterization of this strain showed defects in the cell wall composition, with a reduction in the N-linked mannan content, and the increment in the levels of O-linked mannans, chitin, and β-glucans.
32365492	8	52	theme	wall	1422:1425	arg1	composition					1427:1437	the cell wall composition	1413:1437	the cell wall composition	1413:1437	Collectively, these data showed that loss of Kex2 affected the cell wall composition, the protein glycosylation pathways, and interaction with innate immune cells.
32365492	5	53	theme	mannans	961:967	arg1	levels					942:947	the levels	938:947	the levels of O-linked mannans	938:967	The characterization of this strain showed defects in the cell wall composition, with a reduction in the N-linked mannan content, and the increment in the levels of O-linked mannans, chitin, and β-glucans.
32365492	3	54	theme	previous	489:496	arg1	studies					498:504	These previous studies	483:504	These previous studies	483:504	These previous studies were performed using cells without URA3 or with URA3 ectopically placed into the KEX2 locus.
32365492	1	55	theme	reticulum	205:213	arg1	lumen					180:184	the lumen	176:184	the lumen of the endoplasmic reticulum and transport to the Golgi complex	176:248	The secretory pathway in Candida albicans involves the protein translocation into the lumen of the endoplasmic reticulum and transport to the Golgi complex, where proteins undergo posttranslational modifications, including glycosylation and proteolysis.
32365492	5	56	theme	O-linked	952:959	arg1	mannans					961:967	O-linked mannans	952:967	O-linked mannans	952:967	The characterization of this strain showed defects in the cell wall composition, with a reduction in the N-linked mannan content, and the increment in the levels of O-linked mannans, chitin, and β-glucans.
32365492	8	57	theme	cell	1417:1420	arg1	composition					1427:1437	the cell wall composition	1413:1437	the cell wall composition	1413:1437	Collectively, these data showed that loss of Kex2 affected the cell wall composition, the protein glycosylation pathways, and interaction with innate immune cells.
32365492	6	58	from	changes	1048:1054	arg1	enzymes					1074:1080	Golgi-resident enzymes	1059:1080	Golgi-resident enzymes	1059:1080	The defects in the mannan content are likely linked to changes in Golgi-resident enzymes, as the α-1,2-mannosyltransferase and α-1,6-mannosyltransferase activities were incremented and reduced, respectively.
32365492	4	59	theme	null	722:725	arg1	strain					734:739	a kex2Δ null mutant strain	714:739	a kex2Δ null mutant strain with URA3 placed into the neutral locus RPS1	714:784	Since these conditions are known to affect the cellular fitness and the host-fungus interaction, here we generated a kex2Δ null mutant strain with URA3 placed into the neutral locus RPS1.
32365492	4	60	theme	mutant	727:732	arg1	strain					734:739	a kex2Δ null mutant strain	714:739	a kex2Δ null mutant strain with URA3 placed into the neutral locus RPS1	714:784	Since these conditions are known to affect the cellular fitness and the host-fungus interaction, here we generated a kex2Δ null mutant strain with URA3 placed into the neutral locus RPS1.
32365492	4	61	theme	cellular	646:653	arg1	fitness					655:661	the cellular fitness	642:661	the cellular fitness	642:661	Since these conditions are known to affect the cellular fitness and the host-fungus interaction, here we generated a kex2Δ null mutant strain with URA3 placed into the neutral locus RPS1.
32365492	7	62	theme	mononuclear	1305:1315	arg1	cells					1317:1321	human mononuclear cells	1299:1321	human mononuclear cells	1299:1321	The mutant cells also showed reduced ability to stimulate cytokine production and phagocytosis by human mononuclear cells and macrophages, respectively.
32365492	1	63	theme	Candida	119:125	arg1	albicans					127:134	Candida albicans	119:134	Candida albicans	119:134	The secretory pathway in Candida albicans involves the protein translocation into the lumen of the endoplasmic reticulum and transport to the Golgi complex, where proteins undergo posttranslational modifications, including glycosylation and proteolysis.
32365492	1	64	theme	transport	219:227	arg1	lumen					180:184	the lumen	176:184	the lumen of the endoplasmic reticulum and transport to the Golgi complex	176:248	The secretory pathway in Candida albicans involves the protein translocation into the lumen of the endoplasmic reticulum and transport to the Golgi complex, where proteins undergo posttranslational modifications, including glycosylation and proteolysis.
32365492	8	65	theme	immune	1504:1509	arg1	cells					1511:1515	innate immune cells	1497:1515	innate immune cells	1497:1515	Collectively, these data showed that loss of Kex2 affected the cell wall composition, the protein glycosylation pathways, and interaction with innate immune cells.
32365492	0	66	theme	Kex2	8:11	arg1	Loss					0:3	Loss	0:3	Loss of Kex2	0:11	Loss of Kex2 Affects the Candida albicans Cell Wall and Interaction with Innate Immune Cells.
32365492	7	67	theme	cytokine	1259:1266	arg1	production					1268:1277	cytokine production	1259:1277	cytokine production	1259:1277	The mutant cells also showed reduced ability to stimulate cytokine production and phagocytosis by human mononuclear cells and macrophages, respectively.
32365492	8	68	with	interaction	1480:1490	arg1	cells					1511:1515	innate immune cells	1497:1515	innate immune cells	1497:1515	Collectively, these data showed that loss of Kex2 affected the cell wall composition, the protein glycosylation pathways, and interaction with innate immune cells.
32365492	5	69	link	O-linked	952:959	arg1	mannans					961:967	O-linked mannans	952:967	O-linked mannans	952:967	The characterization of this strain showed defects in the cell wall composition, with a reduction in the N-linked mannan content, and the increment in the levels of O-linked mannans, chitin, and β-glucans.
34287120	11	0	theme	chemotaxonomic	1297:1310	arg1	characteristics					1326:1340	the phenotypic, chemotaxonomic and genotypic characteristics	1281:1340	the phenotypic, chemotaxonomic and genotypic characteristics	1281:1340	Based on the phenotypic, chemotaxonomic and genotypic characteristics, it has been concluded that strains DR6-1T, DR6-2 and DR6-4 represent a novel species of the genus Amycolatopsis for which the name Amycolatopsis dendrobii sp.
34287120	11	1	theme	Amycolatopsis	1441:1453	arg1	species					1420:1426	a novel species	1412:1426	a novel species of the genus Amycolatopsis for which the name Amycolatopsis dendrobii sp	1412:1499	Based on the phenotypic, chemotaxonomic and genotypic characteristics, it has been concluded that strains DR6-1T, DR6-2 and DR6-4 represent a novel species of the genus Amycolatopsis for which the name Amycolatopsis dendrobii sp.
34287120	11	2	theme	genus	1435:1439	arg1	Amycolatopsis					1441:1453	the genus Amycolatopsis	1431:1453	the genus Amycolatopsis	1431:1453	Based on the phenotypic, chemotaxonomic and genotypic characteristics, it has been concluded that strains DR6-1T, DR6-2 and DR6-4 represent a novel species of the genus Amycolatopsis for which the name Amycolatopsis dendrobii sp.
34287120	8	3	theme	 16S	820:823	arg1	similarity					844:853	99.9-100 % 16S rRNA gene sequence similarity	810:853	99.9-100 % 16S rRNA gene sequence similarity	810:853	Strains DR6-1T, DR6-2 and DR6-4 shared 99.9-100 % 16S rRNA gene sequence similarity and were closely related to Amycolatopsis echigonensis JCM 21831T (98.7-98.8%).
34287120	10	4	theme	KCTC	1190:1193	arg1	39515T					1195:1200	Amycolatopsis nivea KCTC 39515T	1170:1200	Amycolatopsis nivea KCTC 39515T	1170:1200	The ANIb and dDDH values between genomic sequences of strain DR6-1T and Amycolatopsis echigonensis JCM21831T, Amycolatopsis rubida JCM 10871T and Amycolatopsis nivea KCTC 39515T were 90.55, 92.25, 92.60%, and 47.20, 52.10 and 52.50%, respectively.
34287120	9	5	theme	genome	951:956	arg1	size					958:961	The approximate genome size	935:961	The approximate genome size of strain DR6-1T	935:978	The approximate genome size of strain DR6-1T was 9.6 Mb with a G+C content of 69.6 mol%.
34287120	2	6	theme	actinomycete	118:129	arg1	strains					131:137	Three novel actinomycete strains	106:137	Three novel actinomycete strains	106:137	Three novel actinomycete strains, designated as DR6-1T, DR6-2 and DR6-4, isolated from the roots of Dendrobium heterocarpum Lindl in Thailand were studied using a polyphasic taxonomic approach.
34287120	7	7	theme	detected	614:621	arg1	diphosphatidylglycerol					641:662	diphosphatidylglycerol	641:662	diphosphatidylglycerol	641:662	The detected polar lipids were diphosphatidylglycerol, hydroxyphosphatidylethanolamine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylglycerol.
34287120	7	7	theme	detected	614:621	arg1	lipids					629:634	The detected polar lipids	610:634	The detected polar lipids	610:634	The detected polar lipids were diphosphatidylglycerol, hydroxyphosphatidylethanolamine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylglycerol.
34287120	6	8	theme	fatty	564:568	arg1	acids					570:574	The predominant cellular fatty acids	539:574	The predominant cellular fatty acids	539:574	The predominant cellular fatty acids were iso-C16 : 0 and iso-C15 : 0.
34287120	6	8	theme	fatty	564:568	arg1	iso-C16 					581:588	iso-C16 	581:588	iso-C16 	581:588	The predominant cellular fatty acids were iso-C16 : 0 and iso-C15 : 0.
34287120	10	9	theme	nivea	1184:1188	arg1	39515T					1195:1200	Amycolatopsis nivea KCTC 39515T	1170:1200	Amycolatopsis nivea KCTC 39515T	1170:1200	The ANIb and dDDH values between genomic sequences of strain DR6-1T and Amycolatopsis echigonensis JCM21831T, Amycolatopsis rubida JCM 10871T and Amycolatopsis nivea KCTC 39515T were 90.55, 92.25, 92.60%, and 47.20, 52.10 and 52.50%, respectively.
34287120	2	10	theme	novel	112:116	arg1	strains					131:137	Three novel actinomycete strains	106:137	Three novel actinomycete strains	106:137	Three novel actinomycete strains, designated as DR6-1T, DR6-2 and DR6-4, isolated from the roots of Dendrobium heterocarpum Lindl in Thailand were studied using a polyphasic taxonomic approach.
34287120	3	11	dep	%	352:352	arg1	w/v					355:357	w/v	355:357	w/v	355:357	The strains grew at 20-37 °C, at pH 5-10 and with 5 % (w/v) NaCl.
34287120	9	12	theme	69.6 mol	1013:1020	arg1	%					1021:1021	69.6 mol%	1013:1021	69.6 mol%	1013:1021	The approximate genome size of strain DR6-1T was 9.6 Mb with a G+C content of 69.6 mol%.
34287120	2	13	theme	taxonomic	280:288	arg1	approach					290:297	a polyphasic taxonomic approach	267:297	a polyphasic taxonomic approach	267:297	Three novel actinomycete strains, designated as DR6-1T, DR6-2 and DR6-4, isolated from the roots of Dendrobium heterocarpum Lindl in Thailand were studied using a polyphasic taxonomic approach.
34287120	6	14	theme	cellular	555:562	arg1	acids					570:574	The predominant cellular fatty acids	539:574	The predominant cellular fatty acids	539:574	The predominant cellular fatty acids were iso-C16 : 0 and iso-C15 : 0.
34287120	6	14	theme	cellular	555:562	arg1	iso-C16 					581:588	iso-C16 	581:588	iso-C16 	581:588	The predominant cellular fatty acids were iso-C16 : 0 and iso-C15 : 0.
34287120	10	15	theme	Amycolatopsis	1170:1182	arg1	39515T					1195:1200	Amycolatopsis nivea KCTC 39515T	1170:1200	Amycolatopsis nivea KCTC 39515T	1170:1200	The ANIb and dDDH values between genomic sequences of strain DR6-1T and Amycolatopsis echigonensis JCM21831T, Amycolatopsis rubida JCM 10871T and Amycolatopsis nivea KCTC 39515T were 90.55, 92.25, 92.60%, and 47.20, 52.10 and 52.50%, respectively.
34287120	8	16	dep	Strains	771:777	arg1	DR6-4					797:801	DR6-4	797:801	DR6-4	797:801	Strains DR6-1T, DR6-2 and DR6-4 shared 99.9-100 % 16S rRNA gene sequence similarity and were closely related to Amycolatopsis echigonensis JCM 21831T (98.7-98.8%).
34287120	8	16	dep	Strains	771:777	arg1	Strains					771:777	Strains DR6-1T, DR6-2 and DR6-4	771:801	Strains DR6-1T, DR6-2 and DR6-4	771:801	Strains DR6-1T, DR6-2 and DR6-4 shared 99.9-100 % 16S rRNA gene sequence similarity and were closely related to Amycolatopsis echigonensis JCM 21831T (98.7-98.8%).
34287120	8	16	dep	Strains	771:777	arg1	DR6-2					787:791	DR6-2	787:791	DR6-2	787:791	Strains DR6-1T, DR6-2 and DR6-4 shared 99.9-100 % 16S rRNA gene sequence similarity and were closely related to Amycolatopsis echigonensis JCM 21831T (98.7-98.8%).
34287120	8	16	dep	Strains	771:777	arg1	DR6-1T					779:784	DR6-1T	779:784	DR6-1T	779:784	Strains DR6-1T, DR6-2 and DR6-4 shared 99.9-100 % 16S rRNA gene sequence similarity and were closely related to Amycolatopsis echigonensis JCM 21831T (98.7-98.8%).
34287120	13	17	theme	type	1524:1527	arg1	DR6-1T					1539:1544	DR6-1T	1539:1544	DR6-1T (=JCM 33742T=KCTC 49546T=TISTR 2840T)	1539:1582	The type strain is DR6-1T (=JCM 33742T=KCTC 49546T=TISTR 2840T).
34287120	13	17	theme	type	1524:1527	arg1	strain					1529:1534	The type strain	1520:1534	The type strain	1520:1534	The type strain is DR6-1T (=JCM 33742T=KCTC 49546T=TISTR 2840T).
34287120	2	18	theme	polyphasic	269:278	arg1	approach					290:297	a polyphasic taxonomic approach	267:297	a polyphasic taxonomic approach	267:297	Three novel actinomycete strains, designated as DR6-1T, DR6-2 and DR6-4, isolated from the roots of Dendrobium heterocarpum Lindl in Thailand were studied using a polyphasic taxonomic approach.
34287120	10	19	theme	ANIb	1028:1031	arg1	90.55					1207:1211	90.55	1207:1211	90.55	1207:1211	The ANIb and dDDH values between genomic sequences of strain DR6-1T and Amycolatopsis echigonensis JCM21831T, Amycolatopsis rubida JCM 10871T and Amycolatopsis nivea KCTC 39515T were 90.55, 92.25, 92.60%, and 47.20, 52.10 and 52.50%, respectively.
34287120	10	19	theme	ANIb	1028:1031	arg1	values					1042:1047	The ANIb and dDDH values	1024:1047	values	1042:1047	The ANIb and dDDH values between genomic sequences of strain DR6-1T and Amycolatopsis echigonensis JCM21831T, Amycolatopsis rubida JCM 10871T and Amycolatopsis nivea KCTC 39515T were 90.55, 92.25, 92.60%, and 47.20, 52.10 and 52.50%, respectively.
34287120	10	20	theme	39515T	1195:1200	arg1	sequences					1065:1073	genomic sequences	1057:1073	genomic sequences of strain DR6-1T and Amycolatopsis echigonensis JCM21831T, Amycolatopsis rubida JCM 10871T and Amycolatopsis nivea KCTC 39515T	1057:1200	The ANIb and dDDH values between genomic sequences of strain DR6-1T and Amycolatopsis echigonensis JCM21831T, Amycolatopsis rubida JCM 10871T and Amycolatopsis nivea KCTC 39515T were 90.55, 92.25, 92.60%, and 47.20, 52.10 and 52.50%, respectively.
34287120	2	21	from	roots	197:201	arg1	Thailand					239:246	Thailand	239:246	Thailand	239:246	Three novel actinomycete strains, designated as DR6-1T, DR6-2 and DR6-4, isolated from the roots of Dendrobium heterocarpum Lindl in Thailand were studied using a polyphasic taxonomic approach.
34287120	10	22	theme	genomic	1057:1063	arg1	sequences					1065:1073	genomic sequences	1057:1073	genomic sequences of strain DR6-1T and Amycolatopsis echigonensis JCM21831T, Amycolatopsis rubida JCM 10871T and Amycolatopsis nivea KCTC 39515T	1057:1200	The ANIb and dDDH values between genomic sequences of strain DR6-1T and Amycolatopsis echigonensis JCM21831T, Amycolatopsis rubida JCM 10871T and Amycolatopsis nivea KCTC 39515T were 90.55, 92.25, 92.60%, and 47.20, 52.10 and 52.50%, respectively.
34287120	8	23	dep	related	872:878	arg1	%					931:931	98.7-98.8%	922:931	98.7-98.8%	922:931	Strains DR6-1T, DR6-2 and DR6-4 shared 99.9-100 % 16S rRNA gene sequence similarity and were closely related to Amycolatopsis echigonensis JCM 21831T (98.7-98.8%).
34287120	9	24	theme	G+C	998:1000	arg1	content					1002:1008	a G+C content	996:1008	a G+C content of 69.6 mol%	996:1021	The approximate genome size of strain DR6-1T was 9.6 Mb with a G+C content of 69.6 mol%.
34287120	11	25	theme	phenotypic	1285:1294	arg1	characteristics					1326:1340	the phenotypic, chemotaxonomic and genotypic characteristics	1281:1340	the phenotypic, chemotaxonomic and genotypic characteristics	1281:1340	Based on the phenotypic, chemotaxonomic and genotypic characteristics, it has been concluded that strains DR6-1T, DR6-2 and DR6-4 represent a novel species of the genus Amycolatopsis for which the name Amycolatopsis dendrobii sp.
34287120	1	26	theme	Dendrobium	75:84	arg1	Lindl					99:103	Dendrobium heterocarpum Lindl	75:103	Dendrobium heterocarpum Lindl	75:103	nov., an endophytic actinomycete isolated from Dendrobium heterocarpum Lindl.
34287120	2	27	from	Lindl	230:234	arg1	Thailand					239:246	Thailand	239:246	Thailand	239:246	Three novel actinomycete strains, designated as DR6-1T, DR6-2 and DR6-4, isolated from the roots of Dendrobium heterocarpum Lindl in Thailand were studied using a polyphasic taxonomic approach.
34287120	10	28	theme	echigonensis	1110:1121	arg1	JCM21831T					1123:1131	strain DR6-1T and Amycolatopsis echigonensis JCM21831T	1078:1131	JCM21831T	1123:1131	The ANIb and dDDH values between genomic sequences of strain DR6-1T and Amycolatopsis echigonensis JCM21831T, Amycolatopsis rubida JCM 10871T and Amycolatopsis nivea KCTC 39515T were 90.55, 92.25, 92.60%, and 47.20, 52.10 and 52.50%, respectively.
34287120	0	29	theme	dendrobii	14:22	arg1	sp					24:25	Amycolatopsis dendrobii sp	0:25	Amycolatopsis dendrobii sp.	0:26	Amycolatopsis dendrobii sp.
34287120	1	30	theme	heterocarpum	86:97	arg1	Lindl					99:103	Dendrobium heterocarpum Lindl	75:103	Dendrobium heterocarpum Lindl	75:103	nov., an endophytic actinomycete isolated from Dendrobium heterocarpum Lindl.
34287120	2	31	attach	isolated	179:186	arg1	roots					197:201	the roots	193:201	the roots of Dendrobium heterocarpum Lindl in Thailand	193:246	Three novel actinomycete strains, designated as DR6-1T, DR6-2 and DR6-4, isolated from the roots of Dendrobium heterocarpum Lindl in Thailand were studied using a polyphasic taxonomic approach.
34287120	2	31	attach	isolated	179:186	arg2	strains					131:137	Three novel actinomycete strains	106:137	Three novel actinomycete strains	106:137	Three novel actinomycete strains, designated as DR6-1T, DR6-2 and DR6-4, isolated from the roots of Dendrobium heterocarpum Lindl in Thailand were studied using a polyphasic taxonomic approach.
34287120	0	32	theme	Amycolatopsis	0:12	arg1	sp					24:25	Amycolatopsis dendrobii sp	0:25	Amycolatopsis dendrobii sp.	0:26	Amycolatopsis dendrobii sp.
34287120	11	33	theme	Amycolatopsis	1474:1486	arg1	sp					1498:1499	the name Amycolatopsis dendrobii sp	1465:1499	the name Amycolatopsis dendrobii sp	1465:1499	Based on the phenotypic, chemotaxonomic and genotypic characteristics, it has been concluded that strains DR6-1T, DR6-2 and DR6-4 represent a novel species of the genus Amycolatopsis for which the name Amycolatopsis dendrobii sp.
34287120	2	34	from	Thailand	239:246	arg1	roots					197:201	the roots	193:201	the roots of Dendrobium heterocarpum Lindl in Thailand	193:246	Three novel actinomycete strains, designated as DR6-1T, DR6-2 and DR6-4, isolated from the roots of Dendrobium heterocarpum Lindl in Thailand were studied using a polyphasic taxonomic approach.
34287120	4	35	contain	contained	371:379	arg1	They					366:369	They	366:369	They	366:369	They contained meso-diaminopimelic acid in the cell-wall peptidoglycan and MK-9(H4) was a major menaquinone.
34287120	4	35	contain	contained	371:379	arg2	acid					401:404	meso-diaminopimelic acid	381:404	meso-diaminopimelic acid in the cell-wall peptidoglycan	381:435	They contained meso-diaminopimelic acid in the cell-wall peptidoglycan and MK-9(H4) was a major menaquinone.
34287120	10	36	theme	DR6-1T	1085:1090	arg1	sequences					1065:1073	genomic sequences	1057:1073	genomic sequences of strain DR6-1T and Amycolatopsis echigonensis JCM21831T, Amycolatopsis rubida JCM 10871T and Amycolatopsis nivea KCTC 39515T	1057:1200	The ANIb and dDDH values between genomic sequences of strain DR6-1T and Amycolatopsis echigonensis JCM21831T, Amycolatopsis rubida JCM 10871T and Amycolatopsis nivea KCTC 39515T were 90.55, 92.25, 92.60%, and 47.20, 52.10 and 52.50%, respectively.
34287120	13	37	theme	49546T=TISTR	1564:1575	arg1	2840T					1577:1581	=JCM 33742T=KCTC 49546T=TISTR 2840T	1547:1581	=JCM 33742T=KCTC 49546T=TISTR 2840T	1547:1581	The type strain is DR6-1T (=JCM 33742T=KCTC 49546T=TISTR 2840T).
34287120	13	37	theme	49546T=TISTR	1564:1575	arg1	DR6-1T					1539:1544	DR6-1T	1539:1544	DR6-1T (=JCM 33742T=KCTC 49546T=TISTR 2840T)	1539:1582	The type strain is DR6-1T (=JCM 33742T=KCTC 49546T=TISTR 2840T).
34287120	5	38	theme	cell	528:531	arg1	wall					533:536	the cell wall	524:536	the cell wall	524:536	Arabinose and galactose were the major sugars in the cell wall.
34287120	10	39	theme	52.50	1250:1254	arg1	%					1255:1255	47.20, 52.10 and 52.50%	1233:1255	%	1255:1255	The ANIb and dDDH values between genomic sequences of strain DR6-1T and Amycolatopsis echigonensis JCM21831T, Amycolatopsis rubida JCM 10871T and Amycolatopsis nivea KCTC 39515T were 90.55, 92.25, 92.60%, and 47.20, 52.10 and 52.50%, respectively.
34287120	2	40	dep	Dendrobium	206:215	arg1	heterocarpum					217:228	heterocarpum	217:228	heterocarpum	217:228	Three novel actinomycete strains, designated as DR6-1T, DR6-2 and DR6-4, isolated from the roots of Dendrobium heterocarpum Lindl in Thailand were studied using a polyphasic taxonomic approach.
34287120	3	41	theme	5 	350:351	arg1	%					352:352	%	352:352	%	352:352	The strains grew at 20-37 °C, at pH 5-10 and with 5 % (w/v) NaCl.
34287120	10	42	theme	strain	1078:1083	arg1	DR6-1T					1085:1090	strain DR6-1T and Amycolatopsis echigonensis JCM21831T	1078:1131	DR6-1T	1085:1090	The ANIb and dDDH values between genomic sequences of strain DR6-1T and Amycolatopsis echigonensis JCM21831T, Amycolatopsis rubida JCM 10871T and Amycolatopsis nivea KCTC 39515T were 90.55, 92.25, 92.60%, and 47.20, 52.10 and 52.50%, respectively.
34287120	7	43	theme	polar	623:627	arg1	diphosphatidylglycerol					641:662	diphosphatidylglycerol	641:662	diphosphatidylglycerol	641:662	The detected polar lipids were diphosphatidylglycerol, hydroxyphosphatidylethanolamine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylglycerol.
34287120	7	43	theme	polar	623:627	arg1	lipids					629:634	The detected polar lipids	610:634	The detected polar lipids	610:634	The detected polar lipids were diphosphatidylglycerol, hydroxyphosphatidylethanolamine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylglycerol.
34287120	8	44	theme	echigonensis	897:908	arg1	21831T					914:919	Amycolatopsis echigonensis JCM 21831T	883:919	Amycolatopsis echigonensis JCM 21831T	883:919	Strains DR6-1T, DR6-2 and DR6-4 shared 99.9-100 % 16S rRNA gene sequence similarity and were closely related to Amycolatopsis echigonensis JCM 21831T (98.7-98.8%).
34287120	9	45	theme	strain	966:971	arg1	DR6-1T					973:978	strain DR6-1T	966:978	strain DR6-1T	966:978	The approximate genome size of strain DR6-1T was 9.6 Mb with a G+C content of 69.6 mol%.
34287120	10	46	theme	47.20	1233:1237	arg1	%					1255:1255	47.20, 52.10 and 52.50%	1233:1255	%	1255:1255	The ANIb and dDDH values between genomic sequences of strain DR6-1T and Amycolatopsis echigonensis JCM21831T, Amycolatopsis rubida JCM 10871T and Amycolatopsis nivea KCTC 39515T were 90.55, 92.25, 92.60%, and 47.20, 52.10 and 52.50%, respectively.
34287120	9	47	theme	approximate	939:949	arg1	size					958:961	The approximate genome size	935:961	The approximate genome size of strain DR6-1T	935:978	The approximate genome size of strain DR6-1T was 9.6 Mb with a G+C content of 69.6 mol%.
34287120	8	48	theme	sequence	835:842	arg1	similarity					844:853	99.9-100 % 16S rRNA gene sequence similarity	810:853	99.9-100 % 16S rRNA gene sequence similarity	810:853	Strains DR6-1T, DR6-2 and DR6-4 shared 99.9-100 % 16S rRNA gene sequence similarity and were closely related to Amycolatopsis echigonensis JCM 21831T (98.7-98.8%).
34287120	2	49	theme	Lindl	230:234	arg1	roots					197:201	the roots	193:201	the roots of Dendrobium heterocarpum Lindl in Thailand	193:246	Three novel actinomycete strains, designated as DR6-1T, DR6-2 and DR6-4, isolated from the roots of Dendrobium heterocarpum Lindl in Thailand were studied using a polyphasic taxonomic approach.
34287120	8	50	theme	Amycolatopsis	883:895	arg1	21831T					914:919	Amycolatopsis echigonensis JCM 21831T	883:919	Amycolatopsis echigonensis JCM 21831T	883:919	Strains DR6-1T, DR6-2 and DR6-4 shared 99.9-100 % 16S rRNA gene sequence similarity and were closely related to Amycolatopsis echigonensis JCM 21831T (98.7-98.8%).
34287120	11	51	theme	name	1469:1472	arg1	sp					1498:1499	the name Amycolatopsis dendrobii sp	1465:1499	the name Amycolatopsis dendrobii sp	1465:1499	Based on the phenotypic, chemotaxonomic and genotypic characteristics, it has been concluded that strains DR6-1T, DR6-2 and DR6-4 represent a novel species of the genus Amycolatopsis for which the name Amycolatopsis dendrobii sp.
34287120	9	52	theme	DR6-1T	973:978	arg1	size					958:961	The approximate genome size	935:961	The approximate genome size of strain DR6-1T	935:978	The approximate genome size of strain DR6-1T was 9.6 Mb with a G+C content of 69.6 mol%.
34287120	4	53	theme	major	456:460	arg1	menaquinone					462:472	a major menaquinone	454:472	a major menaquinone	454:472	They contained meso-diaminopimelic acid in the cell-wall peptidoglycan and MK-9(H4) was a major menaquinone.
34287120	4	53	theme	major	456:460	arg1	MK-9					441:444	MK-9	441:444	MK-9(H4)	441:448	They contained meso-diaminopimelic acid in the cell-wall peptidoglycan and MK-9(H4) was a major menaquinone.
34287120	10	54	theme	JCM21831T	1123:1131	arg1	sequences					1065:1073	genomic sequences	1057:1073	genomic sequences of strain DR6-1T and Amycolatopsis echigonensis JCM21831T, Amycolatopsis rubida JCM 10871T and Amycolatopsis nivea KCTC 39515T	1057:1200	The ANIb and dDDH values between genomic sequences of strain DR6-1T and Amycolatopsis echigonensis JCM21831T, Amycolatopsis rubida JCM 10871T and Amycolatopsis nivea KCTC 39515T were 90.55, 92.25, 92.60%, and 47.20, 52.10 and 52.50%, respectively.
34287120	4	55	from	acid	401:404	arg1	peptidoglycan					423:435	the cell-wall peptidoglycan	409:435	the cell-wall peptidoglycan	409:435	They contained meso-diaminopimelic acid in the cell-wall peptidoglycan and MK-9(H4) was a major menaquinone.
34287120	4	56	theme	cell-wall	413:421	arg1	peptidoglycan					423:435	the cell-wall peptidoglycan	409:435	the cell-wall peptidoglycan	409:435	They contained meso-diaminopimelic acid in the cell-wall peptidoglycan and MK-9(H4) was a major menaquinone.
34287120	2	57	theme	Dendrobium	206:215	arg1	Lindl					230:234	Dendrobium heterocarpum Lindl	206:234	Dendrobium heterocarpum Lindl in Thailand	206:246	Three novel actinomycete strains, designated as DR6-1T, DR6-2 and DR6-4, isolated from the roots of Dendrobium heterocarpum Lindl in Thailand were studied using a polyphasic taxonomic approach.
34287120	8	58	theme	JCM	910:912	arg1	21831T					914:919	Amycolatopsis echigonensis JCM 21831T	883:919	Amycolatopsis echigonensis JCM 21831T	883:919	Strains DR6-1T, DR6-2 and DR6-4 shared 99.9-100 % 16S rRNA gene sequence similarity and were closely related to Amycolatopsis echigonensis JCM 21831T (98.7-98.8%).
34287120	6	59	theme	predominant	543:553	arg1	acids					570:574	The predominant cellular fatty acids	539:574	The predominant cellular fatty acids	539:574	The predominant cellular fatty acids were iso-C16 : 0 and iso-C15 : 0.
34287120	6	59	theme	predominant	543:553	arg1	iso-C16 					581:588	iso-C16 	581:588	iso-C16 	581:588	The predominant cellular fatty acids were iso-C16 : 0 and iso-C15 : 0.
34287120	8	60	theme	gene	830:833	arg1	similarity					844:853	99.9-100 % 16S rRNA gene sequence similarity	810:853	99.9-100 % 16S rRNA gene sequence similarity	810:853	Strains DR6-1T, DR6-2 and DR6-4 shared 99.9-100 % 16S rRNA gene sequence similarity and were closely related to Amycolatopsis echigonensis JCM 21831T (98.7-98.8%).
34287120	11	61	dep	strains	1370:1376	arg1	strains					1370:1376	strains	1370:1376	strains DR6-1T, DR6-2 and DR6-4	1370:1400	Based on the phenotypic, chemotaxonomic and genotypic characteristics, it has been concluded that strains DR6-1T, DR6-2 and DR6-4 represent a novel species of the genus Amycolatopsis for which the name Amycolatopsis dendrobii sp.
34287120	11	61	dep	strains	1370:1376	arg1	DR6-2					1386:1390	DR6-2	1386:1390	DR6-2	1386:1390	Based on the phenotypic, chemotaxonomic and genotypic characteristics, it has been concluded that strains DR6-1T, DR6-2 and DR6-4 represent a novel species of the genus Amycolatopsis for which the name Amycolatopsis dendrobii sp.
34287120	11	61	dep	strains	1370:1376	arg1	DR6-1T					1378:1383	DR6-1T	1378:1383	DR6-1T	1378:1383	Based on the phenotypic, chemotaxonomic and genotypic characteristics, it has been concluded that strains DR6-1T, DR6-2 and DR6-4 represent a novel species of the genus Amycolatopsis for which the name Amycolatopsis dendrobii sp.
34287120	11	61	dep	strains	1370:1376	arg1	DR6-4					1396:1400	DR6-4	1396:1400	DR6-4	1396:1400	Based on the phenotypic, chemotaxonomic and genotypic characteristics, it has been concluded that strains DR6-1T, DR6-2 and DR6-4 represent a novel species of the genus Amycolatopsis for which the name Amycolatopsis dendrobii sp.
34287120	11	62	theme	novel	1414:1418	arg1	species					1420:1426	a novel species	1412:1426	a novel species of the genus Amycolatopsis for which the name Amycolatopsis dendrobii sp	1412:1499	Based on the phenotypic, chemotaxonomic and genotypic characteristics, it has been concluded that strains DR6-1T, DR6-2 and DR6-4 represent a novel species of the genus Amycolatopsis for which the name Amycolatopsis dendrobii sp.
34287120	10	63	theme	Amycolatopsis	1096:1108	arg1	JCM21831T					1123:1131	strain DR6-1T and Amycolatopsis echigonensis JCM21831T	1078:1131	JCM21831T	1123:1131	The ANIb and dDDH values between genomic sequences of strain DR6-1T and Amycolatopsis echigonensis JCM21831T, Amycolatopsis rubida JCM 10871T and Amycolatopsis nivea KCTC 39515T were 90.55, 92.25, 92.60%, and 47.20, 52.10 and 52.50%, respectively.
34287120	13	64	theme	=JCM	1547:1550	arg1	2840T					1577:1581	=JCM 33742T=KCTC 49546T=TISTR 2840T	1547:1581	=JCM 33742T=KCTC 49546T=TISTR 2840T	1547:1581	The type strain is DR6-1T (=JCM 33742T=KCTC 49546T=TISTR 2840T).
34287120	13	64	theme	=JCM	1547:1550	arg1	DR6-1T					1539:1544	DR6-1T	1539:1544	DR6-1T (=JCM 33742T=KCTC 49546T=TISTR 2840T)	1539:1582	The type strain is DR6-1T (=JCM 33742T=KCTC 49546T=TISTR 2840T).
34287120	5	65	theme	major	508:512	arg1	Arabinose					475:483	Arabinose	475:483	Arabinose	475:483	Arabinose and galactose were the major sugars in the cell wall.
34287120	5	65	theme	major	508:512	arg1	sugars					514:519	the major sugars	504:519	the major sugars in the cell wall	504:536	Arabinose and galactose were the major sugars in the cell wall.
34287120	5	65	theme	major	508:512	arg1	galactose					489:497	galactose	489:497	galactose	489:497	Arabinose and galactose were the major sugars in the cell wall.
34287120	5	66	from	sugars	514:519	arg1	wall					533:536	the cell wall	524:536	the cell wall	524:536	Arabinose and galactose were the major sugars in the cell wall.
34287120	9	67	theme	%	1021:1021	arg1	content					1002:1008	a G+C content	996:1008	a G+C content of 69.6 mol%	996:1021	The approximate genome size of strain DR6-1T was 9.6 Mb with a G+C content of 69.6 mol%.
34287120	10	68	theme	10871T	1159:1164	arg1	sequences					1065:1073	genomic sequences	1057:1073	genomic sequences of strain DR6-1T and Amycolatopsis echigonensis JCM21831T, Amycolatopsis rubida JCM 10871T and Amycolatopsis nivea KCTC 39515T	1057:1200	The ANIb and dDDH values between genomic sequences of strain DR6-1T and Amycolatopsis echigonensis JCM21831T, Amycolatopsis rubida JCM 10871T and Amycolatopsis nivea KCTC 39515T were 90.55, 92.25, 92.60%, and 47.20, 52.10 and 52.50%, respectively.
34287120	13	69	theme	33742T=KCTC	1552:1562	arg1	2840T					1577:1581	=JCM 33742T=KCTC 49546T=TISTR 2840T	1547:1581	=JCM 33742T=KCTC 49546T=TISTR 2840T	1547:1581	The type strain is DR6-1T (=JCM 33742T=KCTC 49546T=TISTR 2840T).
34287120	13	69	theme	33742T=KCTC	1552:1562	arg1	DR6-1T					1539:1544	DR6-1T	1539:1544	DR6-1T (=JCM 33742T=KCTC 49546T=TISTR 2840T)	1539:1582	The type strain is DR6-1T (=JCM 33742T=KCTC 49546T=TISTR 2840T).
34287120	8	70	theme	%	819:819	arg1	similarity					844:853	99.9-100 % 16S rRNA gene sequence similarity	810:853	99.9-100 % 16S rRNA gene sequence similarity	810:853	Strains DR6-1T, DR6-2 and DR6-4 shared 99.9-100 % 16S rRNA gene sequence similarity and were closely related to Amycolatopsis echigonensis JCM 21831T (98.7-98.8%).
34287120	10	71	theme	JCM	1155:1157	arg1	10871T					1159:1164	Amycolatopsis rubida JCM 10871T	1134:1164	Amycolatopsis rubida JCM 10871T	1134:1164	The ANIb and dDDH values between genomic sequences of strain DR6-1T and Amycolatopsis echigonensis JCM21831T, Amycolatopsis rubida JCM 10871T and Amycolatopsis nivea KCTC 39515T were 90.55, 92.25, 92.60%, and 47.20, 52.10 and 52.50%, respectively.
34287120	11	72	theme	genotypic	1316:1324	arg1	characteristics					1326:1340	the phenotypic, chemotaxonomic and genotypic characteristics	1281:1340	the phenotypic, chemotaxonomic and genotypic characteristics	1281:1340	Based on the phenotypic, chemotaxonomic and genotypic characteristics, it has been concluded that strains DR6-1T, DR6-2 and DR6-4 represent a novel species of the genus Amycolatopsis for which the name Amycolatopsis dendrobii sp.
34287120	4	73	theme	meso-diaminopimelic	381:399	arg1	acid					401:404	meso-diaminopimelic acid	381:404	meso-diaminopimelic acid in the cell-wall peptidoglycan	381:435	They contained meso-diaminopimelic acid in the cell-wall peptidoglycan and MK-9(H4) was a major menaquinone.
34287120	6	74	dep	iso-C16 	581:588	arg1	 0					606:607	 0	606:607	iso-C16 : 0 and iso-C15 : 0	581:607	The predominant cellular fatty acids were iso-C16 : 0 and iso-C15 : 0.
34287120	6	74	dep	iso-C16 	581:588	arg1	iso-C15 					597:604	iso-C15 	597:604	iso-C15 	597:604	The predominant cellular fatty acids were iso-C16 : 0 and iso-C15 : 0.
34287120	6	74	dep	iso-C16 	581:588	arg1	 0					590:591	 0	590:591	 0	590:591	The predominant cellular fatty acids were iso-C16 : 0 and iso-C15 : 0.
34287120	8	75	theme	99.9-100 	810:818	arg1	%					819:819	%	819:819	%	819:819	Strains DR6-1T, DR6-2 and DR6-4 shared 99.9-100 % 16S rRNA gene sequence similarity and were closely related to Amycolatopsis echigonensis JCM 21831T (98.7-98.8%).
34287120	10	76	theme	rubida	1148:1153	arg1	10871T					1159:1164	Amycolatopsis rubida JCM 10871T	1134:1164	Amycolatopsis rubida JCM 10871T	1134:1164	The ANIb and dDDH values between genomic sequences of strain DR6-1T and Amycolatopsis echigonensis JCM21831T, Amycolatopsis rubida JCM 10871T and Amycolatopsis nivea KCTC 39515T were 90.55, 92.25, 92.60%, and 47.20, 52.10 and 52.50%, respectively.
34287120	10	77	theme	dDDH	1037:1040	arg1	90.55					1207:1211	90.55	1207:1211	90.55	1207:1211	The ANIb and dDDH values between genomic sequences of strain DR6-1T and Amycolatopsis echigonensis JCM21831T, Amycolatopsis rubida JCM 10871T and Amycolatopsis nivea KCTC 39515T were 90.55, 92.25, 92.60%, and 47.20, 52.10 and 52.50%, respectively.
34287120	10	77	theme	dDDH	1037:1040	arg1	values					1042:1047	The ANIb and dDDH values	1024:1047	values	1042:1047	The ANIb and dDDH values between genomic sequences of strain DR6-1T and Amycolatopsis echigonensis JCM21831T, Amycolatopsis rubida JCM 10871T and Amycolatopsis nivea KCTC 39515T were 90.55, 92.25, 92.60%, and 47.20, 52.10 and 52.50%, respectively.
34287120	1	78	theme	endophytic	37:46	arg1	actinomycete					48:59	an endophytic actinomycete	34:59	an endophytic actinomycete	34:59	nov., an endophytic actinomycete isolated from Dendrobium heterocarpum Lindl.
34287120	1	78	theme	endophytic	37:46	arg1	nov.					28:31	nov.	28:31	nov.	28:31	nov., an endophytic actinomycete isolated from Dendrobium heterocarpum Lindl.
34287120	3	79	theme	%	352:352	arg1	NaCl					360:363	5 % (w/v) NaCl	350:363	5 % (w/v) NaCl	350:363	The strains grew at 20-37 °C, at pH 5-10 and with 5 % (w/v) NaCl.
34287120	8	80	theme	rRNA	825:828	arg1	similarity					844:853	99.9-100 % 16S rRNA gene sequence similarity	810:853	99.9-100 % 16S rRNA gene sequence similarity	810:853	Strains DR6-1T, DR6-2 and DR6-4 shared 99.9-100 % 16S rRNA gene sequence similarity and were closely related to Amycolatopsis echigonensis JCM 21831T (98.7-98.8%).
34287120	10	81	theme	Amycolatopsis	1134:1146	arg1	10871T					1159:1164	Amycolatopsis rubida JCM 10871T	1134:1164	Amycolatopsis rubida JCM 10871T	1134:1164	The ANIb and dDDH values between genomic sequences of strain DR6-1T and Amycolatopsis echigonensis JCM21831T, Amycolatopsis rubida JCM 10871T and Amycolatopsis nivea KCTC 39515T were 90.55, 92.25, 92.60%, and 47.20, 52.10 and 52.50%, respectively.
34062160	3	0	theme	different	561:569	arg1	microenvironment					593:608	the different chemical and physical microenvironment	557:608	the different chemical and physical microenvironment of these two hydrogels	557:631	However, the chondro-inductive ability of collagen and hyaluronic acid hydrogel has not been evaluated yet and the different chemical and physical microenvironment of these two hydrogels increase the difficulty of comparison.
34062160	9	1	theme	continuous	1654:1663	arg1	production					1665:1674	continuous production	1654:1674	continuous production of cartilage-related matrix in the later stage	1654:1721	In contrast, relatively stable physical microenvironment of HA helped to maintain continuous production of cartilage-related matrix in the later stage.
34062160	8	2	from	hydrogels	1542:1550	arg1	stage					1565:1569	the later stage	1555:1569	the later stage	1555:1569	However, the severe shrinkage of Col and Cgp resulted in no enough space for cell proliferation within hydrogels in the later stage.
34062160	7	3	theme	HA	1435:1436	arg1	order					1414:1418	the order	1410:1418	the order of Col > Cgp > HA	1410:1436	Thanks to cell condensation was more likely to occur in collagen hydrogels in the early stage, the cartilage-induced ability was in the order of Col > Cgp > HA.
34062160	11	4	theme	successful	2038:2047	arg1	repair					2049:2054	successful repair	2038:2054	successful repair of articular cartilage	2038:2077	However, it provided important information for optimization and design of the future hydrogels towards successful repair of articular cartilage.
34062160	9	5	theme	matrix	1697:1702	arg1	production					1665:1674	continuous production	1654:1674	continuous production of cartilage-related matrix in the later stage	1654:1721	In contrast, relatively stable physical microenvironment of HA helped to maintain continuous production of cartilage-related matrix in the later stage.
34062160	7	6	theme	Cgp	1429:1431	arg1	HA					1435:1436	Col > Cgp > HA	1423:1436	Col > Cgp > HA	1423:1436	Thanks to cell condensation was more likely to occur in collagen hydrogels in the early stage, the cartilage-induced ability was in the order of Col > Cgp > HA.
34062160	5	7	contain	have	1010:1013	arg2	composition					1033:1043	the same chemical composition	1015:1043	the same chemical composition	1015:1043	Col and Cgp have the same chemical composition and similar microstructure, but are different from HA, while Cgp and HA hydrogels have the same mechanical strength.
34062160	5	7	contain	have	1010:1013	arg1	Cgp					1006:1008	Cgp	1006:1008	Cgp	1006:1008	Col and Cgp have the same chemical composition and similar microstructure, but are different from HA, while Cgp and HA hydrogels have the same mechanical strength.
34062160	5	7	contain	have	1010:1013	arg2	microstructure					1057:1070	similar microstructure	1049:1070	similar microstructure	1049:1070	Col and Cgp have the same chemical composition and similar microstructure, but are different from HA, while Cgp and HA hydrogels have the same mechanical strength.
34062160	5	7	contain	have	1010:1013	arg1	Col					998:1000	Col	998:1000	Col	998:1000	Col and Cgp have the same chemical composition and similar microstructure, but are different from HA, while Cgp and HA hydrogels have the same mechanical strength.
34062160	4	8	theme	hyaluronic	735:744	arg1	acid					746:749	hyaluronic acid	735:749	hyaluronic acid (self-assembled collagen hydrogel (Col), self-assembled collagen hydrogel cross-linked with genipin (Cgp), and methacrylated hyaluronic acid hydrogel (HA))	735:905	In this study, three different hydrogels based on collagen and hyaluronic acid (self-assembled collagen hydrogel (Col), self-assembled collagen hydrogel cross-linked with genipin (Cgp), and methacrylated hyaluronic acid hydrogel (HA)) were prepared and their chondro-inductive ability on the encapsulated BMSCs was evaluated.
34062160	10	9	theme	different	1837:1845	arg1	microenvironment					1869:1884	different chemical and physical microenvironment	1837:1884	different chemical and physical microenvironment	1837:1884	Overall, these results revealed that the chondro-inductive ability of collagen and hyaluronic acid hydrogel with different chemical and physical microenvironment cannot be evaluated by a particular time period.
34062160	10	10	theme	collagen	1794:1801	arg1	ability					1783:1789	the chondro-inductive ability	1761:1789	the chondro-inductive ability of collagen and hyaluronic acid hydrogel with different chemical and physical microenvironment	1761:1884	Overall, these results revealed that the chondro-inductive ability of collagen and hyaluronic acid hydrogel with different chemical and physical microenvironment cannot be evaluated by a particular time period.
34062160	7	11	theme	Col	1423:1425	arg1	HA					1435:1436	Col > Cgp > HA	1423:1436	Col > Cgp > HA	1423:1436	Thanks to cell condensation was more likely to occur in collagen hydrogels in the early stage, the cartilage-induced ability was in the order of Col > Cgp > HA.
34062160	4	12	theme	different	693:701	arg1	hydrogels					703:711	three different hydrogels	687:711	three different hydrogels based on collagen and hyaluronic acid (self-assembled collagen hydrogel (Col), self-assembled collagen hydrogel cross-linked with genipin (Cgp), and methacrylated hyaluronic acid hydrogel (HA))	687:905	In this study, three different hydrogels based on collagen and hyaluronic acid (self-assembled collagen hydrogel (Col), self-assembled collagen hydrogel cross-linked with genipin (Cgp), and methacrylated hyaluronic acid hydrogel (HA)) were prepared and their chondro-inductive ability on the encapsulated BMSCs was evaluated.
34062160	5	13	theme	Cgp	1106:1108	arg1	hydrogels					1117:1125	Cgp and HA hydrogels	1106:1125	Cgp and HA hydrogels	1106:1125	Col and Cgp have the same chemical composition and similar microstructure, but are different from HA, while Cgp and HA hydrogels have the same mechanical strength.
34062160	3	14	theme	hydrogels	623:631	arg1	microenvironment					593:608	the different chemical and physical microenvironment	557:608	the different chemical and physical microenvironment of these two hydrogels	557:631	However, the chondro-inductive ability of collagen and hyaluronic acid hydrogel has not been evaluated yet and the different chemical and physical microenvironment of these two hydrogels increase the difficulty of comparison.
34062160	3	15	theme	collagen	488:495	arg1	ability					477:483	the chondro-inductive ability	455:483	the chondro-inductive ability of collagen and hyaluronic acid hydrogel	455:524	However, the chondro-inductive ability of collagen and hyaluronic acid hydrogel has not been evaluated yet and the different chemical and physical microenvironment of these two hydrogels increase the difficulty of comparison.
34062160	5	16	theme	HA	1114:1115	arg1	hydrogels					1117:1125	Cgp and HA hydrogels	1106:1125	Cgp and HA hydrogels	1106:1125	Col and Cgp have the same chemical composition and similar microstructure, but are different from HA, while Cgp and HA hydrogels have the same mechanical strength.
34062160	10	17	theme	hydrogel	1823:1830	arg1	ability					1783:1789	the chondro-inductive ability	1761:1789	the chondro-inductive ability of collagen and hyaluronic acid hydrogel with different chemical and physical microenvironment	1761:1884	Overall, these results revealed that the chondro-inductive ability of collagen and hyaluronic acid hydrogel with different chemical and physical microenvironment cannot be evaluated by a particular time period.
34062160	9	18	from	production	1665:1674	arg1	stage					1717:1721	the later stage	1707:1721	the later stage	1707:1721	In contrast, relatively stable physical microenvironment of HA helped to maintain continuous production of cartilage-related matrix in the later stage.
34062160	10	19	theme	hyaluronic	1807:1816	arg1	hydrogel					1823:1830	hyaluronic acid hydrogel	1807:1830	hyaluronic acid hydrogel	1807:1830	Overall, these results revealed that the chondro-inductive ability of collagen and hyaluronic acid hydrogel with different chemical and physical microenvironment cannot be evaluated by a particular time period.
34062160	8	20	theme	Col	1472:1474	arg1	shrinkage					1459:1467	the severe shrinkage	1448:1467	the severe shrinkage of Col and Cgp	1448:1482	However, the severe shrinkage of Col and Cgp resulted in no enough space for cell proliferation within hydrogels in the later stage.
34062160	4	21	from	ability	949:955	arg1	BMSCs					977:981	the encapsulated BMSCs	960:981	the encapsulated BMSCs	960:981	In this study, three different hydrogels based on collagen and hyaluronic acid (self-assembled collagen hydrogel (Col), self-assembled collagen hydrogel cross-linked with genipin (Cgp), and methacrylated hyaluronic acid hydrogel (HA)) were prepared and their chondro-inductive ability on the encapsulated BMSCs was evaluated.
34062160	11	22	theme	hydrogels	2020:2028	arg1	design					1999:2004	design	1999:2004	design	1999:2004	However, it provided important information for optimization and design of the future hydrogels towards successful repair of articular cartilage.
34062160	11	22	theme	hydrogels	2020:2028	arg1	optimization					1982:1993	optimization	1982:1993	optimization	1982:1993	However, it provided important information for optimization and design of the future hydrogels towards successful repair of articular cartilage.
34062160	1	23	theme	marrow	172:177	arg1	differentiation					148:162	chondrogenic differentiation	135:162	chondrogenic differentiation of bone marrow mesenchymal stem cells (BMSCs)	135:208	Achieving chondrogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) successfully is crucial for cartilage regeneration.
34062160	8	24	theme	Cgp	1480:1482	arg1	shrinkage					1459:1467	the severe shrinkage	1448:1467	the severe shrinkage of Col and Cgp	1448:1482	However, the severe shrinkage of Col and Cgp resulted in no enough space for cell proliferation within hydrogels in the later stage.
34062160	2	25	theme	various	271:277	arg1	hydrogels					279:287	various hydrogels	271:287	various hydrogels with different chemical microenvironment	271:328	To date, various hydrogels with different chemical microenvironment have been used to modulate chondrogenic differentiation of BMSCs, especially collagen and hyaluronic acid hydrogel.
34062160	1	26	theme	stem	191:194	arg1	BMSCs					203:207	BMSCs	203:207	BMSCs	203:207	Achieving chondrogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) successfully is crucial for cartilage regeneration.
34062160	1	26	theme	stem	191:194	arg1	cells					196:200	mesenchymal stem cells	179:200	bone marrow mesenchymal stem cells (BMSCs)	167:208	Achieving chondrogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) successfully is crucial for cartilage regeneration.
34062160	4	27	theme	collagen	767:774	arg1	Col					786:788	Col	786:788	Col	786:788	In this study, three different hydrogels based on collagen and hyaluronic acid (self-assembled collagen hydrogel (Col), self-assembled collagen hydrogel cross-linked with genipin (Cgp), and methacrylated hyaluronic acid hydrogel (HA)) were prepared and their chondro-inductive ability on the encapsulated BMSCs was evaluated.
34062160	4	27	theme	collagen	767:774	arg1	hydrogel					776:783	self-assembled collagen hydrogel	752:783	self-assembled collagen hydrogel (Col)	752:789	In this study, three different hydrogels based on collagen and hyaluronic acid (self-assembled collagen hydrogel (Col), self-assembled collagen hydrogel cross-linked with genipin (Cgp), and methacrylated hyaluronic acid hydrogel (HA)) were prepared and their chondro-inductive ability on the encapsulated BMSCs was evaluated.
34062160	5	28	from	HA	1096:1097	arg1	different					1081:1089	different	1081:1089	different	1081:1089	Col and Cgp have the same chemical composition and similar microstructure, but are different from HA, while Cgp and HA hydrogels have the same mechanical strength.
34062160	10	29	with	ability	1783:1789	arg1	microenvironment					1869:1884	different chemical and physical microenvironment	1837:1884	different chemical and physical microenvironment	1837:1884	Overall, these results revealed that the chondro-inductive ability of collagen and hyaluronic acid hydrogel with different chemical and physical microenvironment cannot be evaluated by a particular time period.
34062160	5	30	theme	same	1136:1139	arg1	strength					1152:1159	the same mechanical strength	1132:1159	the same mechanical strength	1132:1159	Col and Cgp have the same chemical composition and similar microstructure, but are different from HA, while Cgp and HA hydrogels have the same mechanical strength.
34062160	4	31	dep	acid	746:749	arg1	Col					786:788	Col	786:788	Col	786:788	In this study, three different hydrogels based on collagen and hyaluronic acid (self-assembled collagen hydrogel (Col), self-assembled collagen hydrogel cross-linked with genipin (Cgp), and methacrylated hyaluronic acid hydrogel (HA)) were prepared and their chondro-inductive ability on the encapsulated BMSCs was evaluated.
34062160	4	31	dep	acid	746:749	arg1	HA					902:903	HA	902:903	HA	902:903	In this study, three different hydrogels based on collagen and hyaluronic acid (self-assembled collagen hydrogel (Col), self-assembled collagen hydrogel cross-linked with genipin (Cgp), and methacrylated hyaluronic acid hydrogel (HA)) were prepared and their chondro-inductive ability on the encapsulated BMSCs was evaluated.
34062160	4	31	dep	acid	746:749	arg1	hydrogel					892:899	methacrylated hyaluronic acid hydrogel	862:899	methacrylated hyaluronic acid hydrogel (HA)	862:904	In this study, three different hydrogels based on collagen and hyaluronic acid (self-assembled collagen hydrogel (Col), self-assembled collagen hydrogel cross-linked with genipin (Cgp), and methacrylated hyaluronic acid hydrogel (HA)) were prepared and their chondro-inductive ability on the encapsulated BMSCs was evaluated.
34062160	4	31	dep	acid	746:749	arg1	Cgp					852:854	Cgp	852:854	Cgp	852:854	In this study, three different hydrogels based on collagen and hyaluronic acid (self-assembled collagen hydrogel (Col), self-assembled collagen hydrogel cross-linked with genipin (Cgp), and methacrylated hyaluronic acid hydrogel (HA)) were prepared and their chondro-inductive ability on the encapsulated BMSCs was evaluated.
34062160	4	31	dep	acid	746:749	arg1	hydrogel					816:823	self-assembled collagen hydrogel	792:823	self-assembled collagen hydrogel cross-linked with genipin (Cgp)	792:855	In this study, three different hydrogels based on collagen and hyaluronic acid (self-assembled collagen hydrogel (Col), self-assembled collagen hydrogel cross-linked with genipin (Cgp), and methacrylated hyaluronic acid hydrogel (HA)) were prepared and their chondro-inductive ability on the encapsulated BMSCs was evaluated.
34062160	4	31	dep	acid	746:749	arg1	hydrogel					776:783	self-assembled collagen hydrogel	752:783	self-assembled collagen hydrogel (Col)	752:789	In this study, three different hydrogels based on collagen and hyaluronic acid (self-assembled collagen hydrogel (Col), self-assembled collagen hydrogel cross-linked with genipin (Cgp), and methacrylated hyaluronic acid hydrogel (HA)) were prepared and their chondro-inductive ability on the encapsulated BMSCs was evaluated.
34062160	4	32	theme	self-assembled	792:805	arg1	Cgp					852:854	Cgp	852:854	Cgp	852:854	In this study, three different hydrogels based on collagen and hyaluronic acid (self-assembled collagen hydrogel (Col), self-assembled collagen hydrogel cross-linked with genipin (Cgp), and methacrylated hyaluronic acid hydrogel (HA)) were prepared and their chondro-inductive ability on the encapsulated BMSCs was evaluated.
34062160	4	32	theme	self-assembled	792:805	arg1	hydrogel					816:823	self-assembled collagen hydrogel	792:823	self-assembled collagen hydrogel cross-linked with genipin (Cgp)	792:855	In this study, three different hydrogels based on collagen and hyaluronic acid (self-assembled collagen hydrogel (Col), self-assembled collagen hydrogel cross-linked with genipin (Cgp), and methacrylated hyaluronic acid hydrogel (HA)) were prepared and their chondro-inductive ability on the encapsulated BMSCs was evaluated.
34062160	7	33	theme	early	1360:1364	arg1	stage					1366:1370	the early stage	1356:1370	the early stage	1356:1370	Thanks to cell condensation was more likely to occur in collagen hydrogels in the early stage, the cartilage-induced ability was in the order of Col > Cgp > HA.
34062160	11	34	theme	important	1956:1964	arg1	information					1966:1976	important information	1956:1976	important information for optimization and design of the future hydrogels towards successful repair of articular cartilage	1956:2077	However, it provided important information for optimization and design of the future hydrogels towards successful repair of articular cartilage.
34062160	0	35	theme	chondro-inductivity	14:32	arg1	Comparison					0:9	Comparison	0:9	Comparison of chondro-inductivity between collagen and hyaluronic acid hydrogel	0:78	Comparison of chondro-inductivity between collagen and hyaluronic acid hydrogel based on chemical/physical microenvironment.
34062160	1	36	theme	chondrogenic	135:146	arg1	differentiation					148:162	chondrogenic differentiation	135:162	chondrogenic differentiation of bone marrow mesenchymal stem cells (BMSCs)	135:208	Achieving chondrogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) successfully is crucial for cartilage regeneration.
34062160	3	37	theme	chondro-inductive	459:475	arg1	ability					477:483	the chondro-inductive ability	455:483	the chondro-inductive ability of collagen and hyaluronic acid hydrogel	455:524	However, the chondro-inductive ability of collagen and hyaluronic acid hydrogel has not been evaluated yet and the different chemical and physical microenvironment of these two hydrogels increase the difficulty of comparison.
34062160	4	38	theme	hyaluronic	876:885	arg1	HA					902:903	HA	902:903	HA	902:903	In this study, three different hydrogels based on collagen and hyaluronic acid (self-assembled collagen hydrogel (Col), self-assembled collagen hydrogel cross-linked with genipin (Cgp), and methacrylated hyaluronic acid hydrogel (HA)) were prepared and their chondro-inductive ability on the encapsulated BMSCs was evaluated.
34062160	4	38	theme	hyaluronic	876:885	arg1	hydrogel					892:899	methacrylated hyaluronic acid hydrogel	862:899	methacrylated hyaluronic acid hydrogel (HA)	862:904	In this study, three different hydrogels based on collagen and hyaluronic acid (self-assembled collagen hydrogel (Col), self-assembled collagen hydrogel cross-linked with genipin (Cgp), and methacrylated hyaluronic acid hydrogel (HA)) were prepared and their chondro-inductive ability on the encapsulated BMSCs was evaluated.
34062160	3	39	theme	acid	512:515	arg1	ability					477:483	the chondro-inductive ability	455:483	the chondro-inductive ability of collagen and hyaluronic acid hydrogel	455:524	However, the chondro-inductive ability of collagen and hyaluronic acid hydrogel has not been evaluated yet and the different chemical and physical microenvironment of these two hydrogels increase the difficulty of comparison.
34062160	3	40	dep	collagen	488:495	arg1	hydrogel					517:524	hydrogel	517:524	hydrogel	517:524	However, the chondro-inductive ability of collagen and hyaluronic acid hydrogel has not been evaluated yet and the different chemical and physical microenvironment of these two hydrogels increase the difficulty of comparison.
34062160	7	41	theme	collagen	1334:1341	arg1	hydrogels					1343:1351	collagen hydrogels	1334:1351	collagen hydrogels	1334:1351	Thanks to cell condensation was more likely to occur in collagen hydrogels in the early stage, the cartilage-induced ability was in the order of Col > Cgp > HA.
34062160	0	42	theme	hyaluronic	55:64	arg1	hydrogel					71:78	hyaluronic acid hydrogel	55:78	hyaluronic acid hydrogel	55:78	Comparison of chondro-inductivity between collagen and hyaluronic acid hydrogel based on chemical/physical microenvironment.
34062160	9	43	theme	physical	1603:1610	arg1	microenvironment					1612:1627	relatively stable physical microenvironment	1585:1627	relatively stable physical microenvironment of HA	1585:1633	In contrast, relatively stable physical microenvironment of HA helped to maintain continuous production of cartilage-related matrix in the later stage.
34062160	2	44	used	used	340:343	arg2	hydrogels					279:287	various hydrogels	271:287	various hydrogels with different chemical microenvironment	271:328	To date, various hydrogels with different chemical microenvironment have been used to modulate chondrogenic differentiation of BMSCs, especially collagen and hyaluronic acid hydrogel.
34062160	6	45	theme	hydrogels	1227:1235	arg1	microenvironments					1202:1218	chemical and physical microenvironments	1180:1218	chemical and physical microenvironments of the hydrogels combined to influence cell condensation	1180:1275	It was found that chemical and physical microenvironments of the hydrogels combined to influence cell condensation.
34062160	10	46	theme	particular	1911:1920	arg1	period					1927:1932	a particular time period	1909:1932	a particular time period	1909:1932	Overall, these results revealed that the chondro-inductive ability of collagen and hyaluronic acid hydrogel with different chemical and physical microenvironment cannot be evaluated by a particular time period.
34062160	3	47	theme	chemical	571:578	arg1	microenvironment					593:608	the different chemical and physical microenvironment	557:608	the different chemical and physical microenvironment of these two hydrogels	557:631	However, the chondro-inductive ability of collagen and hyaluronic acid hydrogel has not been evaluated yet and the different chemical and physical microenvironment of these two hydrogels increase the difficulty of comparison.
34062160	7	48	theme	cell	1288:1291	arg1	condensation					1293:1304	cell condensation	1288:1304	cell condensation was more likely to occur in collagen hydrogels in the early stage	1288:1370	Thanks to cell condensation was more likely to occur in collagen hydrogels in the early stage, the cartilage-induced ability was in the order of Col > Cgp > HA.
34062160	10	49	theme	chondro-inductive	1765:1781	arg1	ability					1783:1789	the chondro-inductive ability	1761:1789	the chondro-inductive ability of collagen and hyaluronic acid hydrogel with different chemical and physical microenvironment	1761:1884	Overall, these results revealed that the chondro-inductive ability of collagen and hyaluronic acid hydrogel with different chemical and physical microenvironment cannot be evaluated by a particular time period.
34062160	3	50	theme	physical	584:591	arg1	microenvironment					593:608	the different chemical and physical microenvironment	557:608	the different chemical and physical microenvironment of these two hydrogels	557:631	However, the chondro-inductive ability of collagen and hyaluronic acid hydrogel has not been evaluated yet and the different chemical and physical microenvironment of these two hydrogels increase the difficulty of comparison.
34062160	2	51	dep	different	294:302	arg1	chemical					304:311	chemical	304:311	chemical	304:311	To date, various hydrogels with different chemical microenvironment have been used to modulate chondrogenic differentiation of BMSCs, especially collagen and hyaluronic acid hydrogel.
34062160	9	52	theme	cartilage-related	1679:1695	arg1	matrix					1697:1702	cartilage-related matrix	1679:1702	cartilage-related matrix	1679:1702	In contrast, relatively stable physical microenvironment of HA helped to maintain continuous production of cartilage-related matrix in the later stage.
34062160	6	53	theme	physical	1193:1200	arg1	microenvironments					1202:1218	chemical and physical microenvironments	1180:1218	chemical and physical microenvironments of the hydrogels combined to influence cell condensation	1180:1275	It was found that chemical and physical microenvironments of the hydrogels combined to influence cell condensation.
34062160	10	54	dep	different	1837:1845	arg1	chemical					1847:1854	chemical	1847:1854	chemical	1847:1854	Overall, these results revealed that the chondro-inductive ability of collagen and hyaluronic acid hydrogel with different chemical and physical microenvironment cannot be evaluated by a particular time period.
34062160	10	54	dep	different	1837:1845	arg1	physical					1860:1867	physical	1860:1867	physical	1860:1867	Overall, these results revealed that the chondro-inductive ability of collagen and hyaluronic acid hydrogel with different chemical and physical microenvironment cannot be evaluated by a particular time period.
34062160	2	55	theme	chondrogenic	357:368	arg1	differentiation					370:384	chondrogenic differentiation	357:384	chondrogenic differentiation of BMSCs, especially collagen and hyaluronic acid hydrogel	357:443	To date, various hydrogels with different chemical microenvironment have been used to modulate chondrogenic differentiation of BMSCs, especially collagen and hyaluronic acid hydrogel.
34062160	6	56	theme	chemical	1180:1187	arg1	microenvironments					1202:1218	chemical and physical microenvironments	1180:1218	chemical and physical microenvironments of the hydrogels combined to influence cell condensation	1180:1275	It was found that chemical and physical microenvironments of the hydrogels combined to influence cell condensation.
34062160	11	57	theme	articular	2059:2067	arg1	cartilage					2069:2077	articular cartilage	2059:2077	articular cartilage	2059:2077	However, it provided important information for optimization and design of the future hydrogels towards successful repair of articular cartilage.
34062160	7	58	theme	>	1433:1433	arg1	HA					1435:1436	Col > Cgp > HA	1423:1436	Col > Cgp > HA	1423:1436	Thanks to cell condensation was more likely to occur in collagen hydrogels in the early stage, the cartilage-induced ability was in the order of Col > Cgp > HA.
34062160	4	59	theme	encapsulated	964:975	arg1	BMSCs					977:981	the encapsulated BMSCs	960:981	the encapsulated BMSCs	960:981	In this study, three different hydrogels based on collagen and hyaluronic acid (self-assembled collagen hydrogel (Col), self-assembled collagen hydrogel cross-linked with genipin (Cgp), and methacrylated hyaluronic acid hydrogel (HA)) were prepared and their chondro-inductive ability on the encapsulated BMSCs was evaluated.
34062160	8	60	theme	severe	1452:1457	arg1	shrinkage					1459:1467	the severe shrinkage	1448:1467	the severe shrinkage of Col and Cgp	1448:1482	However, the severe shrinkage of Col and Cgp resulted in no enough space for cell proliferation within hydrogels in the later stage.
34062160	7	61	theme	>	1427:1427	arg1	HA					1435:1436	Col > Cgp > HA	1423:1436	Col > Cgp > HA	1423:1436	Thanks to cell condensation was more likely to occur in collagen hydrogels in the early stage, the cartilage-induced ability was in the order of Col > Cgp > HA.
34062160	3	62	theme	comparison	660:669	arg1	difficulty					646:655	the difficulty	642:655	the difficulty of comparison	642:669	However, the chondro-inductive ability of collagen and hyaluronic acid hydrogel has not been evaluated yet and the different chemical and physical microenvironment of these two hydrogels increase the difficulty of comparison.
34062160	1	63	theme	cartilage	238:246	arg1	regeneration					248:259	cartilage regeneration	238:259	cartilage regeneration	238:259	Achieving chondrogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) successfully is crucial for cartilage regeneration.
34062160	2	64	with	hydrogels	279:287	arg1	microenvironment					313:328	different chemical microenvironment	294:328	different chemical microenvironment	294:328	To date, various hydrogels with different chemical microenvironment have been used to modulate chondrogenic differentiation of BMSCs, especially collagen and hyaluronic acid hydrogel.
34062160	11	65	theme	future	2013:2018	arg1	hydrogels					2020:2028	the future hydrogels	2009:2028	the future hydrogels	2009:2028	However, it provided important information for optimization and design of the future hydrogels towards successful repair of articular cartilage.
34062160	10	66	theme	acid	1818:1821	arg1	hydrogel					1823:1830	hyaluronic acid hydrogel	1807:1830	hyaluronic acid hydrogel	1807:1830	Overall, these results revealed that the chondro-inductive ability of collagen and hyaluronic acid hydrogel with different chemical and physical microenvironment cannot be evaluated by a particular time period.
34062160	4	67	theme	chondro-inductive	931:947	arg1	ability					949:955	their chondro-inductive ability	925:955	their chondro-inductive ability on the encapsulated BMSCs	925:981	In this study, three different hydrogels based on collagen and hyaluronic acid (self-assembled collagen hydrogel (Col), self-assembled collagen hydrogel cross-linked with genipin (Cgp), and methacrylated hyaluronic acid hydrogel (HA)) were prepared and their chondro-inductive ability on the encapsulated BMSCs was evaluated.
34062160	6	68	theme	cell	1259:1262	arg1	condensation					1264:1275	cell condensation	1259:1275	cell condensation	1259:1275	It was found that chemical and physical microenvironments of the hydrogels combined to influence cell condensation.
34062160	2	69	theme	different	294:302	arg1	microenvironment					313:328	different chemical microenvironment	294:328	different chemical microenvironment	294:328	To date, various hydrogels with different chemical microenvironment have been used to modulate chondrogenic differentiation of BMSCs, especially collagen and hyaluronic acid hydrogel.
34062160	1	70	theme	bone	167:170	arg1	marrow					172:177	bone marrow mesenchymal stem cells (BMSCs)	167:208	bone marrow mesenchymal stem cells (BMSCs)	167:208	Achieving chondrogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) successfully is crucial for cartilage regeneration.
34062160	1	71	theme	mesenchymal	179:189	arg1	BMSCs					203:207	BMSCs	203:207	BMSCs	203:207	Achieving chondrogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) successfully is crucial for cartilage regeneration.
34062160	1	71	theme	mesenchymal	179:189	arg1	cells					196:200	mesenchymal stem cells	179:200	bone marrow mesenchymal stem cells (BMSCs)	167:208	Achieving chondrogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) successfully is crucial for cartilage regeneration.
34062160	7	72	theme	cartilage-induced	1377:1393	arg1	ability					1395:1401	the cartilage-induced ability	1373:1401	the cartilage-induced ability	1373:1401	Thanks to cell condensation was more likely to occur in collagen hydrogels in the early stage, the cartilage-induced ability was in the order of Col > Cgp > HA.
34062160	0	73	theme	chemical/physical	89:105	arg1	microenvironment					107:122	chemical/physical microenvironment	89:122	chemical/physical microenvironment	89:122	Comparison of chondro-inductivity between collagen and hyaluronic acid hydrogel based on chemical/physical microenvironment.
34062160	9	74	theme	later	1711:1715	arg1	stage					1717:1721	the later stage	1707:1721	the later stage	1707:1721	In contrast, relatively stable physical microenvironment of HA helped to maintain continuous production of cartilage-related matrix in the later stage.
34062160	4	75	theme	self-assembled	752:765	arg1	Col					786:788	Col	786:788	Col	786:788	In this study, three different hydrogels based on collagen and hyaluronic acid (self-assembled collagen hydrogel (Col), self-assembled collagen hydrogel cross-linked with genipin (Cgp), and methacrylated hyaluronic acid hydrogel (HA)) were prepared and their chondro-inductive ability on the encapsulated BMSCs was evaluated.
34062160	4	75	theme	self-assembled	752:765	arg1	hydrogel					776:783	self-assembled collagen hydrogel	752:783	self-assembled collagen hydrogel (Col)	752:789	In this study, three different hydrogels based on collagen and hyaluronic acid (self-assembled collagen hydrogel (Col), self-assembled collagen hydrogel cross-linked with genipin (Cgp), and methacrylated hyaluronic acid hydrogel (HA)) were prepared and their chondro-inductive ability on the encapsulated BMSCs was evaluated.
34062160	11	76	theme	cartilage	2069:2077	arg1	repair					2049:2054	successful repair	2038:2054	successful repair of articular cartilage	2038:2077	However, it provided important information for optimization and design of the future hydrogels towards successful repair of articular cartilage.
34062160	8	77	theme	cell	1516:1519	arg1	proliferation					1521:1533	cell proliferation	1516:1533	cell proliferation within hydrogels in the later stage	1516:1569	However, the severe shrinkage of Col and Cgp resulted in no enough space for cell proliferation within hydrogels in the later stage.
34062160	1	78	dep	marrow	172:177	arg1	BMSCs					203:207	BMSCs	203:207	BMSCs	203:207	Achieving chondrogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) successfully is crucial for cartilage regeneration.
34062160	1	78	dep	marrow	172:177	arg1	cells					196:200	mesenchymal stem cells	179:200	bone marrow mesenchymal stem cells (BMSCs)	167:208	Achieving chondrogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) successfully is crucial for cartilage regeneration.
34062160	5	79	theme	same	1019:1022	arg1	composition					1033:1043	the same chemical composition	1015:1043	the same chemical composition	1015:1043	Col and Cgp have the same chemical composition and similar microstructure, but are different from HA, while Cgp and HA hydrogels have the same mechanical strength.
34062160	5	80	contain	have	1127:1130	arg2	strength					1152:1159	the same mechanical strength	1132:1159	the same mechanical strength	1132:1159	Col and Cgp have the same chemical composition and similar microstructure, but are different from HA, while Cgp and HA hydrogels have the same mechanical strength.
34062160	5	80	contain	have	1127:1130	arg1	hydrogels					1117:1125	Cgp and HA hydrogels	1106:1125	Cgp and HA hydrogels	1106:1125	Col and Cgp have the same chemical composition and similar microstructure, but are different from HA, while Cgp and HA hydrogels have the same mechanical strength.
34062160	3	81	theme	hyaluronic	501:510	arg1	acid					512:515	hyaluronic acid	501:515	hyaluronic acid	501:515	However, the chondro-inductive ability of collagen and hyaluronic acid hydrogel has not been evaluated yet and the different chemical and physical microenvironment of these two hydrogels increase the difficulty of comparison.
34062160	5	82	theme	mechanical	1141:1150	arg1	strength					1152:1159	the same mechanical strength	1132:1159	the same mechanical strength	1132:1159	Col and Cgp have the same chemical composition and similar microstructure, but are different from HA, while Cgp and HA hydrogels have the same mechanical strength.
34062160	2	83	theme	acid	431:434	arg1	BMSCs					389:393	BMSCs	389:393	BMSCs	389:393	To date, various hydrogels with different chemical microenvironment have been used to modulate chondrogenic differentiation of BMSCs, especially collagen and hyaluronic acid hydrogel.
34062160	2	83	theme	acid	431:434	arg1	hydrogel					436:443	hyaluronic acid hydrogel	420:443	hyaluronic acid hydrogel	420:443	To date, various hydrogels with different chemical microenvironment have been used to modulate chondrogenic differentiation of BMSCs, especially collagen and hyaluronic acid hydrogel.
34062160	5	84	theme	similar	1049:1055	arg1	microstructure					1057:1070	similar microstructure	1049:1070	similar microstructure	1049:1070	Col and Cgp have the same chemical composition and similar microstructure, but are different from HA, while Cgp and HA hydrogels have the same mechanical strength.
34062160	8	85	theme	later	1559:1563	arg1	stage					1565:1569	the later stage	1555:1569	the later stage	1555:1569	However, the severe shrinkage of Col and Cgp resulted in no enough space for cell proliferation within hydrogels in the later stage.
34062160	4	86	theme	collagen	807:814	arg1	Cgp					852:854	Cgp	852:854	Cgp	852:854	In this study, three different hydrogels based on collagen and hyaluronic acid (self-assembled collagen hydrogel (Col), self-assembled collagen hydrogel cross-linked with genipin (Cgp), and methacrylated hyaluronic acid hydrogel (HA)) were prepared and their chondro-inductive ability on the encapsulated BMSCs was evaluated.
34062160	4	86	theme	collagen	807:814	arg1	hydrogel					816:823	self-assembled collagen hydrogel	792:823	self-assembled collagen hydrogel cross-linked with genipin (Cgp)	792:855	In this study, three different hydrogels based on collagen and hyaluronic acid (self-assembled collagen hydrogel (Col), self-assembled collagen hydrogel cross-linked with genipin (Cgp), and methacrylated hyaluronic acid hydrogel (HA)) were prepared and their chondro-inductive ability on the encapsulated BMSCs was evaluated.
34062160	2	87	theme	hyaluronic	420:429	arg1	acid					431:434	hyaluronic acid	420:434	hyaluronic acid hydrogel	420:443	To date, various hydrogels with different chemical microenvironment have been used to modulate chondrogenic differentiation of BMSCs, especially collagen and hyaluronic acid hydrogel.
34062160	4	88	theme	methacrylated	862:874	arg1	HA					902:903	HA	902:903	HA	902:903	In this study, three different hydrogels based on collagen and hyaluronic acid (self-assembled collagen hydrogel (Col), self-assembled collagen hydrogel cross-linked with genipin (Cgp), and methacrylated hyaluronic acid hydrogel (HA)) were prepared and their chondro-inductive ability on the encapsulated BMSCs was evaluated.
34062160	4	88	theme	methacrylated	862:874	arg1	hydrogel					892:899	methacrylated hyaluronic acid hydrogel	862:899	methacrylated hyaluronic acid hydrogel (HA)	862:904	In this study, three different hydrogels based on collagen and hyaluronic acid (self-assembled collagen hydrogel (Col), self-assembled collagen hydrogel cross-linked with genipin (Cgp), and methacrylated hyaluronic acid hydrogel (HA)) were prepared and their chondro-inductive ability on the encapsulated BMSCs was evaluated.
34062160	0	89	theme	acid	66:69	arg1	hydrogel					71:78	hyaluronic acid hydrogel	55:78	hyaluronic acid hydrogel	55:78	Comparison of chondro-inductivity between collagen and hyaluronic acid hydrogel based on chemical/physical microenvironment.
34062160	9	90	theme	stable	1596:1601	arg1	microenvironment					1612:1627	relatively stable physical microenvironment	1585:1627	relatively stable physical microenvironment of HA	1585:1633	In contrast, relatively stable physical microenvironment of HA helped to maintain continuous production of cartilage-related matrix in the later stage.
34062160	10	91	theme	time	1922:1925	arg1	period					1927:1932	a particular time period	1909:1932	a particular time period	1909:1932	Overall, these results revealed that the chondro-inductive ability of collagen and hyaluronic acid hydrogel with different chemical and physical microenvironment cannot be evaluated by a particular time period.
34062160	4	92	theme	acid	887:890	arg1	HA					902:903	HA	902:903	HA	902:903	In this study, three different hydrogels based on collagen and hyaluronic acid (self-assembled collagen hydrogel (Col), self-assembled collagen hydrogel cross-linked with genipin (Cgp), and methacrylated hyaluronic acid hydrogel (HA)) were prepared and their chondro-inductive ability on the encapsulated BMSCs was evaluated.
34062160	4	92	theme	acid	887:890	arg1	hydrogel					892:899	methacrylated hyaluronic acid hydrogel	862:899	methacrylated hyaluronic acid hydrogel (HA)	862:904	In this study, three different hydrogels based on collagen and hyaluronic acid (self-assembled collagen hydrogel (Col), self-assembled collagen hydrogel cross-linked with genipin (Cgp), and methacrylated hyaluronic acid hydrogel (HA)) were prepared and their chondro-inductive ability on the encapsulated BMSCs was evaluated.
34062160	2	93	theme	BMSCs	389:393	arg1	differentiation					370:384	chondrogenic differentiation	357:384	chondrogenic differentiation of BMSCs, especially collagen and hyaluronic acid hydrogel	357:443	To date, various hydrogels with different chemical microenvironment have been used to modulate chondrogenic differentiation of BMSCs, especially collagen and hyaluronic acid hydrogel.
34062160	5	94	theme	chemical	1024:1031	arg1	composition					1033:1043	the same chemical composition	1015:1043	the same chemical composition	1015:1043	Col and Cgp have the same chemical composition and similar microstructure, but are different from HA, while Cgp and HA hydrogels have the same mechanical strength.
34062160	9	95	theme	HA	1632:1633	arg1	microenvironment					1612:1627	relatively stable physical microenvironment	1585:1627	relatively stable physical microenvironment of HA	1585:1633	In contrast, relatively stable physical microenvironment of HA helped to maintain continuous production of cartilage-related matrix in the later stage.
32739566	0	0	theme	Treg	72:75	arg1	cells					77:81	Treg cells	72:81	Treg cells	72:81	Regulatory effect of Zuojin Pill on correlation with gut microbiota and Treg cells in DSS-induced colitis.
32739566	8	1	theme	PD-L1+	1104:1109	arg1	cells					1116:1120	CD4+CD25+Foxp3+ and PD-L1+ Treg cells	1084:1120	CD4+CD25+Foxp3+ and PD-L1+ Treg cells	1084:1120	Meanwhile, the level of CD4+CD25+Foxp3+ and PD-L1+ Treg cells improved.
32739566	7	2	theme	relative	987:994	arg1	abundance					996:1004	the relative abundance	983:1004	the relative abundance of Actinobacteria, and Sphingobacteriia	983:1044	While the diversity of intestinal microflora was regulated, the relative abundance of Actinobacteria, and Sphingobacteriia was modified.
32739566	11	3	theme	Treg	1373:1376	arg1	cells					1378:1382	Treg cells	1373:1382	Treg cells	1373:1382	CONCLUSIONS ZJP regulates crosstalk between intestinal microflora and Treg cells to attenuate experimental colitis via the PI3K/Akt signaling pathway.
32739566	0	4	from	effect	11:16	arg1	correlation					36:46	correlation	36:46	correlation with gut microbiota and Treg cells in DSS-induced colitis	36:104	Regulatory effect of Zuojin Pill on correlation with gut microbiota and Treg cells in DSS-induced colitis.
32739566	4	5	theme	macroscopic	522:532	arg1	observation					550:560	macroscopic and microscopic observation	522:560	macroscopic and microscopic observation	522:560	In the present study, the therapeutic effect of ZJP was evaluated by macroscopic and microscopic observation; regulatory T (Treg) cells and their subsets were analyzed by flow cytometry; and the composition of gut microbiota was tested by 16S rRNA analysis.
32739566	8	6	theme	cells	1116:1120	arg1	Meanwhile					1060:1068	Meanwhile	1060:1068	Meanwhile	1060:1068	Meanwhile, the level of CD4+CD25+Foxp3+ and PD-L1+ Treg cells improved.
32739566	8	6	theme	cells	1116:1120	arg1	level					1075:1079	the level	1071:1079	the level of CD4+CD25+Foxp3+ and PD-L1+ Treg cells	1071:1120	Meanwhile, the level of CD4+CD25+Foxp3+ and PD-L1+ Treg cells improved.
32739566	6	7	theme	pathological	829:840	arg1	damage					842:847	The pathological damage	825:847	RESULTS The pathological damage	817:847	RESULTS The pathological damage was attenuated and expression of proinflammatory cytokines was decreased.
32739566	3	8	dep	AIM	347:349	arg1	treated					427:433	treated	427:433	were treated with ZJP for 7 d	422:450	AIM OF THE STUDY METHODS: Mice with dextran-sulfate-sodium-induced colitis were treated with ZJP for 7 d.
32739566	4	9	theme	16S	692:694	arg1	analysis					701:708	16S rRNA analysis	692:708	16S rRNA analysis	692:708	In the present study, the therapeutic effect of ZJP was evaluated by macroscopic and microscopic observation; regulatory T (Treg) cells and their subsets were analyzed by flow cytometry; and the composition of gut microbiota was tested by 16S rRNA analysis.
32739566	8	10	theme	Treg	1111:1114	arg1	cells					1116:1120	CD4+CD25+Foxp3+ and PD-L1+ Treg cells	1084:1120	CD4+CD25+Foxp3+ and PD-L1+ Treg cells	1084:1120	Meanwhile, the level of CD4+CD25+Foxp3+ and PD-L1+ Treg cells improved.
32739566	5	11	theme	3-kinase	745:752	arg1	pathway					775:781	the phosphoinostide 3-kinase (PI3K)/Akt signaling pathway	725:781	the phosphoinostide 3-kinase (PI3K)/Akt signaling pathway	725:781	Activation of the phosphoinostide 3-kinase (PI3K)/Akt signaling pathway was observed by western blotting.
32739566	11	12	theme	signaling	1435:1443	arg1	pathway					1445:1451	the PI3K/Akt signaling pathway	1422:1451	the PI3K/Akt signaling pathway	1422:1451	CONCLUSIONS ZJP regulates crosstalk between intestinal microflora and Treg cells to attenuate experimental colitis via the PI3K/Akt signaling pathway.
32739566	1	13	theme	ulcerative	245:254	arg1	UC					265:266	UC	265:266	UC	265:266	ETHNOPHARMACOLOGICAL RELEVANCE As a classic prescription and commercial Chinese patent medicine, Zuojin Pill (ZJP) has been used to treat ulcerative colitis (UC) effectively for many years.
32739566	1	13	theme	ulcerative	245:254	arg1	colitis					256:262	ulcerative colitis	245:262	ulcerative colitis (UC)	245:267	ETHNOPHARMACOLOGICAL RELEVANCE As a classic prescription and commercial Chinese patent medicine, Zuojin Pill (ZJP) has been used to treat ulcerative colitis (UC) effectively for many years.
32739566	5	14	theme	/Akt	760:763	arg1	pathway					775:781	the phosphoinostide 3-kinase (PI3K)/Akt signaling pathway	725:781	the phosphoinostide 3-kinase (PI3K)/Akt signaling pathway	725:781	Activation of the phosphoinostide 3-kinase (PI3K)/Akt signaling pathway was observed by western blotting.
32739566	4	15	theme	Treg	577:580	arg1	cells					583:587	regulatory T (Treg) cells	563:587	regulatory T (Treg) cells	563:587	In the present study, the therapeutic effect of ZJP was evaluated by macroscopic and microscopic observation; regulatory T (Treg) cells and their subsets were analyzed by flow cytometry; and the composition of gut microbiota was tested by 16S rRNA analysis.
32739566	1	16	theme	commercial	168:177	arg1	medicine					194:201	a classic prescription and commercial Chinese patent medicine	141:201	medicine	194:201	ETHNOPHARMACOLOGICAL RELEVANCE As a classic prescription and commercial Chinese patent medicine, Zuojin Pill (ZJP) has been used to treat ulcerative colitis (UC) effectively for many years.
32739566	0	17	theme	DSS-induced	86:96	arg1	colitis					98:104	DSS-induced colitis	86:104	DSS-induced colitis	86:104	Regulatory effect of Zuojin Pill on correlation with gut microbiota and Treg cells in DSS-induced colitis.
32739566	11	18	theme	intestinal	1347:1356	arg1	microflora					1358:1367	intestinal microflora	1347:1367	intestinal microflora	1347:1367	CONCLUSIONS ZJP regulates crosstalk between intestinal microflora and Treg cells to attenuate experimental colitis via the PI3K/Akt signaling pathway.
32739566	9	19	theme	positive	1159:1166	arg1	correlation					1168:1178	a positive correlation	1157:1178	a positive correlation which was analyzed statistically	1157:1211	These changes maintained a positive correlation which was analyzed statistically.
32739566	4	20	theme	rRNA	696:699	arg1	analysis					701:708	16S rRNA analysis	692:708	16S rRNA analysis	692:708	In the present study, the therapeutic effect of ZJP was evaluated by macroscopic and microscopic observation; regulatory T (Treg) cells and their subsets were analyzed by flow cytometry; and the composition of gut microbiota was tested by 16S rRNA analysis.
32739566	1	21	theme	Chinese	179:185	arg1	medicine					194:201	a classic prescription and commercial Chinese patent medicine	141:201	medicine	194:201	ETHNOPHARMACOLOGICAL RELEVANCE As a classic prescription and commercial Chinese patent medicine, Zuojin Pill (ZJP) has been used to treat ulcerative colitis (UC) effectively for many years.
32739566	5	22	theme	phosphoinostide	729:743	arg1	3-kinase					745:752	phosphoinostide 3-kinase	729:752	the phosphoinostide 3-kinase (PI3K)/Akt signaling pathway	725:781	Activation of the phosphoinostide 3-kinase (PI3K)/Akt signaling pathway was observed by western blotting.
32739566	5	22	theme	phosphoinostide	729:743	arg1	PI3K					755:758	PI3K	755:758	PI3K	755:758	Activation of the phosphoinostide 3-kinase (PI3K)/Akt signaling pathway was observed by western blotting.
32739566	7	23	theme	Sphingobacteriia	1029:1044	arg1	abundance					996:1004	the relative abundance	983:1004	the relative abundance of Actinobacteria, and Sphingobacteriia	983:1044	While the diversity of intestinal microflora was regulated, the relative abundance of Actinobacteria, and Sphingobacteriia was modified.
32739566	4	24	theme	microbiota	667:676	arg1	composition					648:658	the composition	644:658	the composition of gut microbiota	644:676	In the present study, the therapeutic effect of ZJP was evaluated by macroscopic and microscopic observation; regulatory T (Treg) cells and their subsets were analyzed by flow cytometry; and the composition of gut microbiota was tested by 16S rRNA analysis.
32739566	11	25	theme	PI3K/Akt	1426:1433	arg1	pathway					1445:1451	the PI3K/Akt signaling pathway	1422:1451	the PI3K/Akt signaling pathway	1422:1451	CONCLUSIONS ZJP regulates crosstalk between intestinal microflora and Treg cells to attenuate experimental colitis via the PI3K/Akt signaling pathway.
32739566	1	26	theme	patent	187:192	arg1	medicine					194:201	a classic prescription and commercial Chinese patent medicine	141:201	medicine	194:201	ETHNOPHARMACOLOGICAL RELEVANCE As a classic prescription and commercial Chinese patent medicine, Zuojin Pill (ZJP) has been used to treat ulcerative colitis (UC) effectively for many years.
32739566	0	27	theme	Regulatory	0:9	arg1	effect					11:16	Regulatory effect	0:16	Regulatory effect of Zuojin Pill on correlation with gut microbiota and Treg cells in DSS-induced colitis.	0:105	Regulatory effect of Zuojin Pill on correlation with gut microbiota and Treg cells in DSS-induced colitis.
32739566	4	28	theme	gut	663:665	arg1	microbiota					667:676	gut microbiota	663:676	gut microbiota	663:676	In the present study, the therapeutic effect of ZJP was evaluated by macroscopic and microscopic observation; regulatory T (Treg) cells and their subsets were analyzed by flow cytometry; and the composition of gut microbiota was tested by 16S rRNA analysis.
32739566	10	29	theme	pathway	1294:1300	arg1	activation					1257:1266	activation	1257:1266	activation of the PI3K/Akt signaling pathway	1257:1300	Our results also showed that ZJP inhibited activation of the PI3K/Akt signaling pathway.
32739566	5	30	theme	western	799:805	arg1	blotting					807:814	western blotting	799:814	western blotting	799:814	Activation of the phosphoinostide 3-kinase (PI3K)/Akt signaling pathway was observed by western blotting.
32739566	0	31	theme	Zuojin	21:26	arg1	Pill					28:31	Zuojin Pill	21:31	Zuojin Pill	21:31	Regulatory effect of Zuojin Pill on correlation with gut microbiota and Treg cells in DSS-induced colitis.
32739566	4	32	theme	ZJP	501:503	arg1	effect					491:496	the therapeutic effect	475:496	the therapeutic effect of ZJP	475:503	In the present study, the therapeutic effect of ZJP was evaluated by macroscopic and microscopic observation; regulatory T (Treg) cells and their subsets were analyzed by flow cytometry; and the composition of gut microbiota was tested by 16S rRNA analysis.
32739566	10	33	theme	signaling	1284:1292	arg1	pathway					1294:1300	the PI3K/Akt signaling pathway	1271:1300	the PI3K/Akt signaling pathway	1271:1300	Our results also showed that ZJP inhibited activation of the PI3K/Akt signaling pathway.
32739566	11	34	theme	CONCLUSIONS	1303:1313	arg1	ZJP					1315:1317	CONCLUSIONS ZJP	1303:1317	CONCLUSIONS ZJP	1303:1317	CONCLUSIONS ZJP regulates crosstalk between intestinal microflora and Treg cells to attenuate experimental colitis via the PI3K/Akt signaling pathway.
32739566	10	35	theme	PI3K/Akt	1275:1282	arg1	pathway					1294:1300	the PI3K/Akt signaling pathway	1271:1300	the PI3K/Akt signaling pathway	1271:1300	Our results also showed that ZJP inhibited activation of the PI3K/Akt signaling pathway.
32739566	1	36	theme	Zuojin	204:209	arg1	prescription					151:162	a classic prescription and commercial Chinese patent medicine	141:201	prescription	151:162	ETHNOPHARMACOLOGICAL RELEVANCE As a classic prescription and commercial Chinese patent medicine, Zuojin Pill (ZJP) has been used to treat ulcerative colitis (UC) effectively for many years.
32739566	1	36	theme	Zuojin	204:209	arg1	ZJP					217:219	ZJP	217:219	ZJP	217:219	ETHNOPHARMACOLOGICAL RELEVANCE As a classic prescription and commercial Chinese patent medicine, Zuojin Pill (ZJP) has been used to treat ulcerative colitis (UC) effectively for many years.
32739566	1	36	theme	Zuojin	204:209	arg1	Pill					211:214	Zuojin Pill	204:214	Zuojin Pill (ZJP)	204:220	ETHNOPHARMACOLOGICAL RELEVANCE As a classic prescription and commercial Chinese patent medicine, Zuojin Pill (ZJP) has been used to treat ulcerative colitis (UC) effectively for many years.
32739566	3	37	theme	METHODS	364:370	arg1	AIM					347:349	AIM	347:349	AIM OF THE STUDY METHODS: Mice with dextran-sulfate-sodium-induced colitis were treated with ZJP for 7 d.	347:451	AIM OF THE STUDY METHODS: Mice with dextran-sulfate-sodium-induced colitis were treated with ZJP for 7 d.
32739566	6	38	theme	cytokines	898:906	arg1	expression					868:877	expression	868:877	expression of proinflammatory cytokines	868:906	RESULTS The pathological damage was attenuated and expression of proinflammatory cytokines was decreased.
32739566	5	39	theme	signaling	765:773	arg1	pathway					775:781	the phosphoinostide 3-kinase (PI3K)/Akt signaling pathway	725:781	the phosphoinostide 3-kinase (PI3K)/Akt signaling pathway	725:781	Activation of the phosphoinostide 3-kinase (PI3K)/Akt signaling pathway was observed by western blotting.
32739566	0	40	theme	Pill	28:31	arg1	effect					11:16	Regulatory effect	0:16	Regulatory effect of Zuojin Pill on correlation with gut microbiota and Treg cells in DSS-induced colitis.	0:105	Regulatory effect of Zuojin Pill on correlation with gut microbiota and Treg cells in DSS-induced colitis.
32739566	4	41	theme	therapeutic	479:489	arg1	effect					491:496	the therapeutic effect	475:496	the therapeutic effect of ZJP	475:503	In the present study, the therapeutic effect of ZJP was evaluated by macroscopic and microscopic observation; regulatory T (Treg) cells and their subsets were analyzed by flow cytometry; and the composition of gut microbiota was tested by 16S rRNA analysis.
32739566	7	42	theme	Actinobacteria	1009:1022	arg1	abundance					996:1004	the relative abundance	983:1004	the relative abundance of Actinobacteria, and Sphingobacteriia	983:1044	While the diversity of intestinal microflora was regulated, the relative abundance of Actinobacteria, and Sphingobacteriia was modified.
32739566	0	43	from	microbiota	57:66	arg1	colitis					98:104	DSS-induced colitis	86:104	DSS-induced colitis	86:104	Regulatory effect of Zuojin Pill on correlation with gut microbiota and Treg cells in DSS-induced colitis.
32739566	11	44	theme	experimental	1397:1408	arg1	colitis					1410:1416	experimental colitis	1397:1416	experimental colitis	1397:1416	CONCLUSIONS ZJP regulates crosstalk between intestinal microflora and Treg cells to attenuate experimental colitis via the PI3K/Akt signaling pathway.
32739566	5	45	theme	pathway	775:781	arg1	Activation					711:720	Activation	711:720	Activation of the phosphoinostide 3-kinase (PI3K)/Akt signaling pathway	711:781	Activation of the phosphoinostide 3-kinase (PI3K)/Akt signaling pathway was observed by western blotting.
32739566	6	46	theme	proinflammatory	882:896	arg1	cytokines					898:906	proinflammatory cytokines	882:906	proinflammatory cytokines	882:906	RESULTS The pathological damage was attenuated and expression of proinflammatory cytokines was decreased.
32739566	3	47	with	Mice	373:376	arg1	colitis					414:420	dextran-sulfate-sodium-induced colitis	383:420	dextran-sulfate-sodium-induced colitis	383:420	AIM OF THE STUDY METHODS: Mice with dextran-sulfate-sodium-induced colitis were treated with ZJP for 7 d.
32739566	1	48	used	used	231:234	arg2	RELEVANCE					128:136	ETHNOPHARMACOLOGICAL RELEVANCE	107:136	ETHNOPHARMACOLOGICAL RELEVANCE As a classic prescription and commercial Chinese patent medicine, Zuojin Pill (ZJP)	107:220	ETHNOPHARMACOLOGICAL RELEVANCE As a classic prescription and commercial Chinese patent medicine, Zuojin Pill (ZJP) has been used to treat ulcerative colitis (UC) effectively for many years.
32739566	3	49	theme	STUDY	358:362	arg1	METHODS					364:370	THE STUDY METHODS	354:370	THE STUDY METHODS	354:370	AIM OF THE STUDY METHODS: Mice with dextran-sulfate-sodium-induced colitis were treated with ZJP for 7 d.
32739566	1	50	theme	many	285:288	arg1	years					290:294	many years	285:294	many years	285:294	ETHNOPHARMACOLOGICAL RELEVANCE As a classic prescription and commercial Chinese patent medicine, Zuojin Pill (ZJP) has been used to treat ulcerative colitis (UC) effectively for many years.
32739566	7	51	theme	intestinal	946:955	arg1	microflora					957:966	intestinal microflora	946:966	intestinal microflora	946:966	While the diversity of intestinal microflora was regulated, the relative abundance of Actinobacteria, and Sphingobacteriia was modified.
32739566	1	52	theme	ETHNOPHARMACOLOGICAL	107:126	arg1	RELEVANCE					128:136	ETHNOPHARMACOLOGICAL RELEVANCE	107:136	ETHNOPHARMACOLOGICAL RELEVANCE As a classic prescription and commercial Chinese patent medicine, Zuojin Pill (ZJP)	107:220	ETHNOPHARMACOLOGICAL RELEVANCE As a classic prescription and commercial Chinese patent medicine, Zuojin Pill (ZJP) has been used to treat ulcerative colitis (UC) effectively for many years.
32739566	3	53	theme	dextran-sulfate-sodium-induced	383:412	arg1	colitis					414:420	dextran-sulfate-sodium-induced colitis	383:420	dextran-sulfate-sodium-induced colitis	383:420	AIM OF THE STUDY METHODS: Mice with dextran-sulfate-sodium-induced colitis were treated with ZJP for 7 d.
32739566	7	54	theme	microflora	957:966	arg1	diversity					933:941	the diversity	929:941	the diversity of intestinal microflora	929:966	While the diversity of intestinal microflora was regulated, the relative abundance of Actinobacteria, and Sphingobacteriia was modified.
32739566	4	55	theme	regulatory	563:572	arg1	cells					583:587	regulatory T (Treg) cells	563:587	regulatory T (Treg) cells	563:587	In the present study, the therapeutic effect of ZJP was evaluated by macroscopic and microscopic observation; regulatory T (Treg) cells and their subsets were analyzed by flow cytometry; and the composition of gut microbiota was tested by 16S rRNA analysis.
32739566	1	56	theme	classic	143:149	arg1	Pill					211:214	Zuojin Pill	204:214	Zuojin Pill (ZJP)	204:220	ETHNOPHARMACOLOGICAL RELEVANCE As a classic prescription and commercial Chinese patent medicine, Zuojin Pill (ZJP) has been used to treat ulcerative colitis (UC) effectively for many years.
32739566	1	56	theme	classic	143:149	arg1	prescription					151:162	a classic prescription and commercial Chinese patent medicine	141:201	prescription	151:162	ETHNOPHARMACOLOGICAL RELEVANCE As a classic prescription and commercial Chinese patent medicine, Zuojin Pill (ZJP) has been used to treat ulcerative colitis (UC) effectively for many years.
32739566	8	57	theme	CD4+CD25+Foxp3+	1084:1098	arg1	cells					1116:1120	CD4+CD25+Foxp3+ and PD-L1+ Treg cells	1084:1120	CD4+CD25+Foxp3+ and PD-L1+ Treg cells	1084:1120	Meanwhile, the level of CD4+CD25+Foxp3+ and PD-L1+ Treg cells improved.
32739566	0	58	from	cells	77:81	arg1	colitis					98:104	DSS-induced colitis	86:104	DSS-induced colitis	86:104	Regulatory effect of Zuojin Pill on correlation with gut microbiota and Treg cells in DSS-induced colitis.
32739566	0	59	with	correlation	36:46	arg1	microbiota					57:66	gut microbiota	53:66	gut microbiota	53:66	Regulatory effect of Zuojin Pill on correlation with gut microbiota and Treg cells in DSS-induced colitis.
32739566	0	59	with	correlation	36:46	arg1	cells					77:81	Treg cells	72:81	Treg cells	72:81	Regulatory effect of Zuojin Pill on correlation with gut microbiota and Treg cells in DSS-induced colitis.
32739566	4	60	theme	flow	624:627	arg1	cytometry					629:637	flow cytometry	624:637	flow cytometry	624:637	In the present study, the therapeutic effect of ZJP was evaluated by macroscopic and microscopic observation; regulatory T (Treg) cells and their subsets were analyzed by flow cytometry; and the composition of gut microbiota was tested by 16S rRNA analysis.
32739566	4	61	theme	microscopic	538:548	arg1	observation					550:560	macroscopic and microscopic observation	522:560	macroscopic and microscopic observation	522:560	In the present study, the therapeutic effect of ZJP was evaluated by macroscopic and microscopic observation; regulatory T (Treg) cells and their subsets were analyzed by flow cytometry; and the composition of gut microbiota was tested by 16S rRNA analysis.
32739566	0	62	theme	gut	53:55	arg1	microbiota					57:66	gut microbiota	53:66	gut microbiota	53:66	Regulatory effect of Zuojin Pill on correlation with gut microbiota and Treg cells in DSS-induced colitis.
32739566	4	63	theme	present	460:466	arg1	study					468:472	the present study	456:472	the present study	456:472	In the present study, the therapeutic effect of ZJP was evaluated by macroscopic and microscopic observation; regulatory T (Treg) cells and their subsets were analyzed by flow cytometry; and the composition of gut microbiota was tested by 16S rRNA analysis.
32739566	6	64	dep	RESULTS	817:823	arg1	damage					842:847	The pathological damage	825:847	RESULTS The pathological damage	817:847	RESULTS The pathological damage was attenuated and expression of proinflammatory cytokines was decreased.
32739566	2	65	theme	action	323:328	arg1	mechanism					310:318	its mechanism	306:318	its mechanism of action	306:328	However, its mechanism of action remains unclear.
32739566	4	66	theme	T	574:574	arg1	cells					583:587	regulatory T (Treg) cells	563:587	regulatory T (Treg) cells	563:587	In the present study, the therapeutic effect of ZJP was evaluated by macroscopic and microscopic observation; regulatory T (Treg) cells and their subsets were analyzed by flow cytometry; and the composition of gut microbiota was tested by 16S rRNA analysis.
34277461	6	0	theme	rDNA	968:971	arg1	sequencing					973:982	16S rDNA sequencing	964:982	16S rDNA sequencing of fecal samples	964:999	16S rDNA sequencing of fecal samples was conducted to determine the changes in gut microbial composition.
34277461	8	1	from	AS+CIH	1261:1266	arg1	bacteria					1217:1224	The gut bacteria	1209:1224	The gut bacteria	1209:1224	The gut bacteria was significantly varied in AS and AS+CIH mice compared with that in the control mice.
34277461	8	1	from	AS+CIH	1261:1266	arg1	varied					1244:1249	varied	1244:1249	varied	1244:1249	The gut bacteria was significantly varied in AS and AS+CIH mice compared with that in the control mice.
34277461	2	2	theme	intestinal	370:379	arg1	microbiota					381:390	intestinal microbiota	370:390	intestinal microbiota	370:390	However, whether intestinal microbiota is implicated in the mechanisms linking CIH to arteriosclerosis (AS) pathogenesis remains unclear.
34277461	8	3	from	AS	1254:1255	arg1	bacteria					1217:1224	The gut bacteria	1209:1224	The gut bacteria	1209:1224	The gut bacteria was significantly varied in AS and AS+CIH mice compared with that in the control mice.
34277461	8	3	from	AS	1254:1255	arg1	varied					1244:1249	varied	1244:1249	varied	1244:1249	The gut bacteria was significantly varied in AS and AS+CIH mice compared with that in the control mice.
34277461	12	4	theme	CIH-induced	1853:1863	arg1	dysbiosis					1868:1876	CIH-induced GM dysbiosis	1853:1876	CIH-induced GM dysbiosis	1853:1876	The correlation of intestinal bacterial parameters with pathological changes in artery indicated complicated interactions under CIH-induced GM dysbiosis.
34277461	14	5	from	features	2243:2250	arg1	development					2258:2268	AS development	2255:2268	AS development	2255:2268	Our findings demonstrated a causal effect of CIH on GM alterations in AS mice and suggested that the disordered GM features in AS development were deteriorated by CIH, which may be associated with AS aggravation.
34277461	9	6	theme	phylum	1479:1484	arg1	levels					1496:1501	phylum and genus levels	1479:1501	levels	1496:1501	Significantly perturbed GM profiles were detected in AS mice with and without CIH, with altered microbial α- and β- diversity and shifts in bacterial compositions at phylum and genus levels.
34277461	8	7	theme	control	1299:1305	arg1	mice					1307:1310	the control mice	1295:1310	the control mice	1295:1310	The gut bacteria was significantly varied in AS and AS+CIH mice compared with that in the control mice.
34277461	13	8	theme	gut	1896:1898	arg1	functions					1910:1918	the gut microbial functions	1892:1918	the gut microbial functions in the potential ability of replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins	1892:2077	Furthermore, the gut microbial functions in the potential ability of replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins were identified to be further suppressed by CIH.
34277461	7	9	theme	apoE-/-	1178:1184	arg1	mice					1186:1189	apoE-/- mice	1178:1189	apoE-/- mice treated with HFD	1178:1206	Morphometric analysis demonstrated that CIH caused aggravated atherosclerotic lesions and facilitated AS in apoE-/- mice treated with HFD.
34277461	11	10	theme	Sutterella	1618:1627	arg1	Aggravation					1595:1605	Aggravation	1595:1605	Aggravation of reduced Sutterella and increased Halomonas, Halomonadaceae and Oceanospirillales	1595:1689	Aggravation of reduced Sutterella and increased Halomonas, Halomonadaceae and Oceanospirillales was noted in CIH-treated AS mice.
34277461	5	11	theme	lesion	906:911	arg1	formation					913:921	atherosclerotic lesion formation	890:921	atherosclerotic lesion formation	890:921	The physiological status and atherosclerotic lesion formation were confirmed by histological analysis.
34277461	10	12	theme	taxa	1582:1585	arg1	levels					1587:1592	different bacteria taxa levels	1563:1592	different bacteria taxa levels	1563:1592	While the difference between AS and AS+CIH was observed at different bacteria taxa levels.
34277461	4	13	theme	apoE-/-	723:729	arg1	mice					732:735	E-deficient (apoE-/-) mice	710:735	E-deficient (apoE-/-) mice treated with high-fat diet (HFD) and subjected to CIH conditions	710:800	Animal models of apolipoprotein E-deficient (apoE-/-) mice treated with high-fat diet (HFD) and subjected to CIH conditions was applied to mimic the AS observed in patients with OSA.
34277461	5	14	theme	physiological	865:877	arg1	status					879:884	The physiological status	861:884	The physiological status	861:884	The physiological status and atherosclerotic lesion formation were confirmed by histological analysis.
34277461	14	15	theme	GM	2240:2241	arg1	features					2243:2250	the disordered GM features	2225:2250	the disordered GM features in AS development	2225:2268	Our findings demonstrated a causal effect of CIH on GM alterations in AS mice and suggested that the disordered GM features in AS development were deteriorated by CIH, which may be associated with AS aggravation.
34277461	3	16	with	association	495:505	arg1	development					523:533	the development	519:533	the development of altered gut microbiota (GM)	519:564	The association of CIH with the development of altered gut microbiota (GM) may provide the opportunity to develop preventive strategies for atherosclerotic cardiovascular risk reduction.
34277461	7	17	theme	Morphometric	1070:1081	arg1	analysis					1083:1090	Morphometric analysis	1070:1090	Morphometric analysis	1070:1090	Morphometric analysis demonstrated that CIH caused aggravated atherosclerotic lesions and facilitated AS in apoE-/- mice treated with HFD.
34277461	1	18	theme	apnea	234:238	arg1	pathophysiology					246:260	highly prevalent obstructive sleep apnea (OSA) pathophysiology	199:260	highly prevalent obstructive sleep apnea (OSA) pathophysiology	199:260	Chronic intermittent hypoxia (CIH) is the prominent signature of highly prevalent obstructive sleep apnea (OSA) pathophysiology, which leads to increased risk and aggravation of atherosclerotic cardiovascular diseases.
34277461	6	19	theme	samples	993:999	arg1	sequencing					973:982	16S rDNA sequencing	964:982	16S rDNA sequencing of fecal samples	964:999	16S rDNA sequencing of fecal samples was conducted to determine the changes in gut microbial composition.
34277461	12	20	from	changes	1794:1800	arg1	artery					1805:1810	artery	1805:1810	artery	1805:1810	The correlation of intestinal bacterial parameters with pathological changes in artery indicated complicated interactions under CIH-induced GM dysbiosis.
34277461	1	21	theme	intermittent	142:153	arg1	hypoxia					155:161	Chronic intermittent hypoxia	134:161	Chronic intermittent hypoxia (CIH)	134:167	Chronic intermittent hypoxia (CIH) is the prominent signature of highly prevalent obstructive sleep apnea (OSA) pathophysiology, which leads to increased risk and aggravation of atherosclerotic cardiovascular diseases.
34277461	1	21	theme	intermittent	142:153	arg1	signature					186:194	the prominent signature	172:194	the prominent signature	172:194	Chronic intermittent hypoxia (CIH) is the prominent signature of highly prevalent obstructive sleep apnea (OSA) pathophysiology, which leads to increased risk and aggravation of atherosclerotic cardiovascular diseases.
34277461	1	21	theme	intermittent	142:153	arg1	CIH					164:166	CIH	164:166	CIH	164:166	Chronic intermittent hypoxia (CIH) is the prominent signature of highly prevalent obstructive sleep apnea (OSA) pathophysiology, which leads to increased risk and aggravation of atherosclerotic cardiovascular diseases.
34277461	10	22	located	observed	1551:1558	arg2	difference					1514:1523	the difference	1510:1523	the difference between AS and AS+CIH	1510:1545	While the difference between AS and AS+CIH was observed at different bacteria taxa levels.
34277461	10	22	located	observed	1551:1558	arg1	levels					1587:1592	different bacteria taxa levels	1563:1592	different bacteria taxa levels	1563:1592	While the difference between AS and AS+CIH was observed at different bacteria taxa levels.
34277461	9	23	theme	GM	1337:1338	arg1	profiles					1340:1347	Significantly perturbed GM profiles	1313:1347	Significantly perturbed GM profiles	1313:1347	Significantly perturbed GM profiles were detected in AS mice with and without CIH, with altered microbial α- and β- diversity and shifts in bacterial compositions at phylum and genus levels.
34277461	3	24	theme	risk	662:665	arg1	reduction					667:675	atherosclerotic cardiovascular risk reduction	631:675	atherosclerotic cardiovascular risk reduction	631:675	The association of CIH with the development of altered gut microbiota (GM) may provide the opportunity to develop preventive strategies for atherosclerotic cardiovascular risk reduction.
34277461	3	25	theme	atherosclerotic	631:645	arg1	reduction					667:675	atherosclerotic cardiovascular risk reduction	631:675	atherosclerotic cardiovascular risk reduction	631:675	The association of CIH with the development of altered gut microbiota (GM) may provide the opportunity to develop preventive strategies for atherosclerotic cardiovascular risk reduction.
34277461	12	26	theme	bacterial	1755:1763	arg1	parameters					1765:1774	intestinal bacterial parameters	1744:1774	intestinal bacterial parameters	1744:1774	The correlation of intestinal bacterial parameters with pathological changes in artery indicated complicated interactions under CIH-induced GM dysbiosis.
34277461	13	27	theme	glycan	1995:2000	arg1	biosynthesis					2002:2013	glycan biosynthesis	1995:2013	glycan biosynthesis	1995:2013	Furthermore, the gut microbial functions in the potential ability of replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins were identified to be further suppressed by CIH.
34277461	13	27	theme	glycan	1995:2000	arg1	proteins					1985:1992	replication recombination and repair proteins	1948:1992	replication recombination and repair proteins	1948:1992	Furthermore, the gut microbial functions in the potential ability of replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins were identified to be further suppressed by CIH.
34277461	15	28	theme	AS	2445:2446	arg1	intervention					2417:2428	intervention	2417:2428	intervention of OSA-related AS	2417:2446	Preventative strategies targeting gut microbiome are highly recommended for intervention of OSA-related AS.
34277461	6	29	theme	gut	1043:1045	arg1	composition					1057:1067	gut microbial composition	1043:1067	gut microbial composition	1043:1067	16S rDNA sequencing of fecal samples was conducted to determine the changes in gut microbial composition.
34277461	4	30	theme	E-deficient	710:720	arg1	mice					732:735	E-deficient (apoE-/-) mice	710:735	E-deficient (apoE-/-) mice treated with high-fat diet (HFD) and subjected to CIH conditions	710:800	Animal models of apolipoprotein E-deficient (apoE-/-) mice treated with high-fat diet (HFD) and subjected to CIH conditions was applied to mimic the AS observed in patients with OSA.
34277461	9	31	from	α-	1419:1420	arg1	compositions					1463:1474	bacterial compositions	1453:1474	bacterial compositions	1453:1474	Significantly perturbed GM profiles were detected in AS mice with and without CIH, with altered microbial α- and β- diversity and shifts in bacterial compositions at phylum and genus levels.
34277461	14	32	theme	AS	2198:2199	arg1	mice					2201:2204	AS mice	2198:2204	AS mice	2198:2204	Our findings demonstrated a causal effect of CIH on GM alterations in AS mice and suggested that the disordered GM features in AS development were deteriorated by CIH, which may be associated with AS aggravation.
34277461	0	33	theme	Microbiota	122:131	arg1	Formation					98:106	the Formation	94:106	the Formation of Intestinal Microbiota	94:131	Chronic Intermittent Hypoxia Participates in the Pathogenesis of Atherosclerosis and Perturbs the Formation of Intestinal Microbiota.
34277461	13	34	theme	proteins	1985:1992	arg1	ability					1937:1943	the potential ability	1923:1943	the potential ability of replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins	1923:2077	Furthermore, the gut microbial functions in the potential ability of replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins were identified to be further suppressed by CIH.
34277461	0	35	theme	Chronic	0:6	arg1	Intermittent Hypoxia					8:27	Chronic Intermittent Hypoxia	0:27	Chronic Intermittent Hypoxia	0:27	Chronic Intermittent Hypoxia Participates in the Pathogenesis of Atherosclerosis and Perturbs the Formation of Intestinal Microbiota.
34277461	1	36	theme	increased	278:286	arg1	risk					288:291	increased risk	278:291	increased risk	278:291	Chronic intermittent hypoxia (CIH) is the prominent signature of highly prevalent obstructive sleep apnea (OSA) pathophysiology, which leads to increased risk and aggravation of atherosclerotic cardiovascular diseases.
34277461	15	37	theme	Preventative	2341:2352	arg1	strategies					2354:2363	Preventative strategies	2341:2363	Preventative strategies targeting gut microbiome	2341:2388	Preventative strategies targeting gut microbiome are highly recommended for intervention of OSA-related AS.
34277461	13	38	theme	replication	1948:1958	arg1	biosynthesis					2002:2013	glycan biosynthesis	1995:2013	glycan biosynthesis	1995:2013	Furthermore, the gut microbial functions in the potential ability of replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins were identified to be further suppressed by CIH.
34277461	13	38	theme	replication	1948:1958	arg1	metabolism					2019:2028	metabolism	2019:2028	metabolism	2019:2028	Furthermore, the gut microbial functions in the potential ability of replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins were identified to be further suppressed by CIH.
34277461	13	38	theme	replication	1948:1958	arg1	proteins					1985:1992	replication recombination and repair proteins	1948:1992	replication recombination and repair proteins	1948:1992	Furthermore, the gut microbial functions in the potential ability of replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins were identified to be further suppressed by CIH.
34277461	1	39	theme	prominent	176:184	arg1	hypoxia					155:161	Chronic intermittent hypoxia	134:161	Chronic intermittent hypoxia (CIH)	134:167	Chronic intermittent hypoxia (CIH) is the prominent signature of highly prevalent obstructive sleep apnea (OSA) pathophysiology, which leads to increased risk and aggravation of atherosclerotic cardiovascular diseases.
34277461	1	39	theme	prominent	176:184	arg1	signature					186:194	the prominent signature	172:194	the prominent signature	172:194	Chronic intermittent hypoxia (CIH) is the prominent signature of highly prevalent obstructive sleep apnea (OSA) pathophysiology, which leads to increased risk and aggravation of atherosclerotic cardiovascular diseases.
34277461	9	40	theme	microbial	1409:1417	arg1	α-					1419:1420	altered microbial α-	1401:1420	altered microbial α-	1401:1420	Significantly perturbed GM profiles were detected in AS mice with and without CIH, with altered microbial α- and β- diversity and shifts in bacterial compositions at phylum and genus levels.
34277461	12	41	with	correlation	1729:1739	arg1	changes					1794:1800	pathological changes	1781:1800	pathological changes in artery	1781:1810	The correlation of intestinal bacterial parameters with pathological changes in artery indicated complicated interactions under CIH-induced GM dysbiosis.
34277461	10	42	theme	bacteria	1573:1580	arg1	levels					1587:1592	different bacteria taxa levels	1563:1592	different bacteria taxa levels	1563:1592	While the difference between AS and AS+CIH was observed at different bacteria taxa levels.
34277461	11	43	theme	Halomonadaceae	1654:1667	arg1	Aggravation					1595:1605	Aggravation	1595:1605	Aggravation of reduced Sutterella and increased Halomonas, Halomonadaceae and Oceanospirillales	1595:1689	Aggravation of reduced Sutterella and increased Halomonas, Halomonadaceae and Oceanospirillales was noted in CIH-treated AS mice.
34277461	1	44	theme	cardiovascular	328:341	arg1	diseases					343:350	atherosclerotic cardiovascular diseases	312:350	atherosclerotic cardiovascular diseases	312:350	Chronic intermittent hypoxia (CIH) is the prominent signature of highly prevalent obstructive sleep apnea (OSA) pathophysiology, which leads to increased risk and aggravation of atherosclerotic cardiovascular diseases.
34277461	11	45	theme	Halomonas	1643:1651	arg1	Aggravation					1595:1605	Aggravation	1595:1605	Aggravation of reduced Sutterella and increased Halomonas, Halomonadaceae and Oceanospirillales	1595:1689	Aggravation of reduced Sutterella and increased Halomonas, Halomonadaceae and Oceanospirillales was noted in CIH-treated AS mice.
34277461	9	46	from	levels	1496:1501	arg1	shifts					1443:1448	shifts	1443:1448	shifts in bacterial compositions at phylum and genus levels	1443:1501	Significantly perturbed GM profiles were detected in AS mice with and without CIH, with altered microbial α- and β- diversity and shifts in bacterial compositions at phylum and genus levels.
34277461	9	46	from	levels	1496:1501	arg1	α-					1419:1420	altered microbial α-	1401:1420	altered microbial α-	1401:1420	Significantly perturbed GM profiles were detected in AS mice with and without CIH, with altered microbial α- and β- diversity and shifts in bacterial compositions at phylum and genus levels.
34277461	9	46	from	levels	1496:1501	arg1	diversity					1429:1437	β- diversity	1426:1437	β- diversity	1426:1437	Significantly perturbed GM profiles were detected in AS mice with and without CIH, with altered microbial α- and β- diversity and shifts in bacterial compositions at phylum and genus levels.
34277461	13	47	theme	microbial	1900:1908	arg1	functions					1910:1918	the gut microbial functions	1892:1918	the gut microbial functions in the potential ability of replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins	1892:2077	Furthermore, the gut microbial functions in the potential ability of replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins were identified to be further suppressed by CIH.
34277461	3	48	theme	altered	538:544	arg1	GM					562:563	GM	562:563	GM	562:563	The association of CIH with the development of altered gut microbiota (GM) may provide the opportunity to develop preventive strategies for atherosclerotic cardiovascular risk reduction.
34277461	3	48	theme	altered	538:544	arg1	microbiota					550:559	altered gut microbiota	538:559	altered gut microbiota (GM)	538:564	The association of CIH with the development of altered gut microbiota (GM) may provide the opportunity to develop preventive strategies for atherosclerotic cardiovascular risk reduction.
34277461	9	49	theme	genus	1490:1494	arg1	levels					1496:1501	phylum and genus levels	1479:1501	levels	1496:1501	Significantly perturbed GM profiles were detected in AS mice with and without CIH, with altered microbial α- and β- diversity and shifts in bacterial compositions at phylum and genus levels.
34277461	11	50	theme	Oceanospirillales	1673:1689	arg1	Aggravation					1595:1605	Aggravation	1595:1605	Aggravation of reduced Sutterella and increased Halomonas, Halomonadaceae and Oceanospirillales	1595:1689	Aggravation of reduced Sutterella and increased Halomonas, Halomonadaceae and Oceanospirillales was noted in CIH-treated AS mice.
34277461	14	51	theme	causal	2156:2161	arg1	effect					2163:2168	a causal effect	2154:2168	a causal effect of CIH on GM alterations in AS mice	2154:2204	Our findings demonstrated a causal effect of CIH on GM alterations in AS mice and suggested that the disordered GM features in AS development were deteriorated by CIH, which may be associated with AS aggravation.
34277461	9	52	theme	bacterial	1453:1461	arg1	compositions					1463:1474	bacterial compositions	1453:1474	bacterial compositions	1453:1474	Significantly perturbed GM profiles were detected in AS mice with and without CIH, with altered microbial α- and β- diversity and shifts in bacterial compositions at phylum and genus levels.
34277461	8	53	theme	gut	1213:1215	arg1	bacteria					1217:1224	The gut bacteria	1209:1224	The gut bacteria	1209:1224	The gut bacteria was significantly varied in AS and AS+CIH mice compared with that in the control mice.
34277461	8	53	theme	gut	1213:1215	arg1	varied					1244:1249	varied	1244:1249	varied	1244:1249	The gut bacteria was significantly varied in AS and AS+CIH mice compared with that in the control mice.
34277461	6	54	theme	16S	964:966	arg1	sequencing					973:982	16S rDNA sequencing	964:982	16S rDNA sequencing of fecal samples	964:999	16S rDNA sequencing of fecal samples was conducted to determine the changes in gut microbial composition.
34277461	12	55	theme	GM	1865:1866	arg1	dysbiosis					1868:1876	CIH-induced GM dysbiosis	1853:1876	CIH-induced GM dysbiosis	1853:1876	The correlation of intestinal bacterial parameters with pathological changes in artery indicated complicated interactions under CIH-induced GM dysbiosis.
34277461	11	56	theme	CIH-treated	1704:1714	arg1	mice					1719:1722	CIH-treated AS mice	1704:1722	CIH-treated AS mice	1704:1722	Aggravation of reduced Sutterella and increased Halomonas, Halomonadaceae and Oceanospirillales was noted in CIH-treated AS mice.
34277461	13	57	theme	vitamins	2070:2077	arg1	biosynthesis					2002:2013	glycan biosynthesis	1995:2013	glycan biosynthesis	1995:2013	Furthermore, the gut microbial functions in the potential ability of replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins were identified to be further suppressed by CIH.
34277461	13	57	theme	vitamins	2070:2077	arg1	metabolism					2042:2051	metabolism	2042:2051	replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins	1948:2077	Furthermore, the gut microbial functions in the potential ability of replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins were identified to be further suppressed by CIH.
34277461	13	57	theme	vitamins	2070:2077	arg1	metabolism					2019:2028	metabolism	2019:2028	metabolism	2019:2028	Furthermore, the gut microbial functions in the potential ability of replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins were identified to be further suppressed by CIH.
34277461	13	57	theme	vitamins	2070:2077	arg1	proteins					1985:1992	replication recombination and repair proteins	1948:1992	replication recombination and repair proteins	1948:1992	Furthermore, the gut microbial functions in the potential ability of replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins were identified to be further suppressed by CIH.
34277461	14	58	from	alterations	2183:2193	arg1	mice					2201:2204	AS mice	2198:2204	AS mice	2198:2204	Our findings demonstrated a causal effect of CIH on GM alterations in AS mice and suggested that the disordered GM features in AS development were deteriorated by CIH, which may be associated with AS aggravation.
34277461	9	59	located	detected	1354:1361	arg2	profiles					1340:1347	Significantly perturbed GM profiles	1313:1347	Significantly perturbed GM profiles	1313:1347	Significantly perturbed GM profiles were detected in AS mice with and without CIH, with altered microbial α- and β- diversity and shifts in bacterial compositions at phylum and genus levels.
34277461	9	59	located	detected	1354:1361	arg1	mice					1369:1372	AS mice	1366:1372	AS mice with and without CIH	1366:1393	Significantly perturbed GM profiles were detected in AS mice with and without CIH, with altered microbial α- and β- diversity and shifts in bacterial compositions at phylum and genus levels.
34277461	3	60	theme	microbiota	550:559	arg1	development					523:533	the development	519:533	the development of altered gut microbiota (GM)	519:564	The association of CIH with the development of altered gut microbiota (GM) may provide the opportunity to develop preventive strategies for atherosclerotic cardiovascular risk reduction.
34277461	5	61	theme	atherosclerotic	890:904	arg1	formation					913:921	atherosclerotic lesion formation	890:921	atherosclerotic lesion formation	890:921	The physiological status and atherosclerotic lesion formation were confirmed by histological analysis.
34277461	12	62	theme	complicated	1822:1832	arg1	interactions					1834:1845	complicated interactions	1822:1845	complicated interactions	1822:1845	The correlation of intestinal bacterial parameters with pathological changes in artery indicated complicated interactions under CIH-induced GM dysbiosis.
34277461	13	63	theme	metabolism	2042:2051	arg1	ability					1937:1943	the potential ability	1923:1943	the potential ability of replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins	1923:2077	Furthermore, the gut microbial functions in the potential ability of replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins were identified to be further suppressed by CIH.
34277461	4	64	dep	mimic	817:821	arg1	the AS observed					823:837	the AS observed	823:837	to mimic the AS observed in patients with OSA	814:858	Animal models of apolipoprotein E-deficient (apoE-/-) mice treated with high-fat diet (HFD) and subjected to CIH conditions was applied to mimic the AS observed in patients with OSA.
34277461	14	65	theme	AS	2255:2256	arg1	development					2258:2268	AS development	2255:2268	AS development	2255:2268	Our findings demonstrated a causal effect of CIH on GM alterations in AS mice and suggested that the disordered GM features in AS development were deteriorated by CIH, which may be associated with AS aggravation.
34277461	13	66	theme	cofactors	2056:2064	arg1	biosynthesis					2002:2013	glycan biosynthesis	1995:2013	glycan biosynthesis	1995:2013	Furthermore, the gut microbial functions in the potential ability of replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins were identified to be further suppressed by CIH.
34277461	13	66	theme	cofactors	2056:2064	arg1	metabolism					2042:2051	metabolism	2042:2051	replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins	1948:2077	Furthermore, the gut microbial functions in the potential ability of replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins were identified to be further suppressed by CIH.
34277461	13	66	theme	cofactors	2056:2064	arg1	metabolism					2019:2028	metabolism	2019:2028	metabolism	2019:2028	Furthermore, the gut microbial functions in the potential ability of replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins were identified to be further suppressed by CIH.
34277461	13	66	theme	cofactors	2056:2064	arg1	proteins					1985:1992	replication recombination and repair proteins	1948:1992	replication recombination and repair proteins	1948:1992	Furthermore, the gut microbial functions in the potential ability of replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins were identified to be further suppressed by CIH.
34277461	1	67	theme	prevalent	206:214	arg1	pathophysiology					246:260	highly prevalent obstructive sleep apnea (OSA) pathophysiology	199:260	highly prevalent obstructive sleep apnea (OSA) pathophysiology	199:260	Chronic intermittent hypoxia (CIH) is the prominent signature of highly prevalent obstructive sleep apnea (OSA) pathophysiology, which leads to increased risk and aggravation of atherosclerotic cardiovascular diseases.
34277461	7	68	theme	aggravated	1121:1130	arg1	lesions					1148:1154	aggravated atherosclerotic lesions	1121:1154	aggravated atherosclerotic lesions	1121:1154	Morphometric analysis demonstrated that CIH caused aggravated atherosclerotic lesions and facilitated AS in apoE-/- mice treated with HFD.
34277461	3	69	theme	preventive	605:614	arg1	strategies					616:625	preventive strategies	605:625	preventive strategies for atherosclerotic cardiovascular risk reduction	605:675	The association of CIH with the development of altered gut microbiota (GM) may provide the opportunity to develop preventive strategies for atherosclerotic cardiovascular risk reduction.
34277461	1	70	theme	sleep	228:232	arg1	OSA					241:243	OSA	241:243	OSA	241:243	Chronic intermittent hypoxia (CIH) is the prominent signature of highly prevalent obstructive sleep apnea (OSA) pathophysiology, which leads to increased risk and aggravation of atherosclerotic cardiovascular diseases.
34277461	1	70	theme	sleep	228:232	arg1	apnea					234:238	obstructive sleep apnea	216:238	highly prevalent obstructive sleep apnea (OSA) pathophysiology	199:260	Chronic intermittent hypoxia (CIH) is the prominent signature of highly prevalent obstructive sleep apnea (OSA) pathophysiology, which leads to increased risk and aggravation of atherosclerotic cardiovascular diseases.
34277461	1	71	theme	diseases	343:350	arg1	aggravation					297:307	aggravation	297:307	aggravation	297:307	Chronic intermittent hypoxia (CIH) is the prominent signature of highly prevalent obstructive sleep apnea (OSA) pathophysiology, which leads to increased risk and aggravation of atherosclerotic cardiovascular diseases.
34277461	1	71	theme	diseases	343:350	arg1	risk					288:291	increased risk	278:291	increased risk	278:291	Chronic intermittent hypoxia (CIH) is the prominent signature of highly prevalent obstructive sleep apnea (OSA) pathophysiology, which leads to increased risk and aggravation of atherosclerotic cardiovascular diseases.
34277461	14	72	theme	disordered	2229:2238	arg1	features					2243:2250	the disordered GM features	2225:2250	the disordered GM features in AS development	2225:2268	Our findings demonstrated a causal effect of CIH on GM alterations in AS mice and suggested that the disordered GM features in AS development were deteriorated by CIH, which may be associated with AS aggravation.
34277461	1	73	theme	Chronic	134:140	arg1	hypoxia					155:161	Chronic intermittent hypoxia	134:161	Chronic intermittent hypoxia (CIH)	134:167	Chronic intermittent hypoxia (CIH) is the prominent signature of highly prevalent obstructive sleep apnea (OSA) pathophysiology, which leads to increased risk and aggravation of atherosclerotic cardiovascular diseases.
34277461	1	73	theme	Chronic	134:140	arg1	signature					186:194	the prominent signature	172:194	the prominent signature	172:194	Chronic intermittent hypoxia (CIH) is the prominent signature of highly prevalent obstructive sleep apnea (OSA) pathophysiology, which leads to increased risk and aggravation of atherosclerotic cardiovascular diseases.
34277461	1	73	theme	Chronic	134:140	arg1	CIH					164:166	CIH	164:166	CIH	164:166	Chronic intermittent hypoxia (CIH) is the prominent signature of highly prevalent obstructive sleep apnea (OSA) pathophysiology, which leads to increased risk and aggravation of atherosclerotic cardiovascular diseases.
34277461	6	74	theme	fecal	987:991	arg1	samples					993:999	fecal samples	987:999	fecal samples	987:999	16S rDNA sequencing of fecal samples was conducted to determine the changes in gut microbial composition.
34277461	4	75	theme	high-fat	750:757	arg1	diet					759:762	high-fat diet	750:762	high-fat diet (HFD)	750:768	Animal models of apolipoprotein E-deficient (apoE-/-) mice treated with high-fat diet (HFD) and subjected to CIH conditions was applied to mimic the AS observed in patients with OSA.
34277461	4	75	theme	high-fat	750:757	arg1	HFD					765:767	HFD	765:767	HFD	765:767	Animal models of apolipoprotein E-deficient (apoE-/-) mice treated with high-fat diet (HFD) and subjected to CIH conditions was applied to mimic the AS observed in patients with OSA.
34277461	9	76	theme	perturbed	1327:1335	arg1	profiles					1340:1347	Significantly perturbed GM profiles	1313:1347	Significantly perturbed GM profiles	1313:1347	Significantly perturbed GM profiles were detected in AS mice with and without CIH, with altered microbial α- and β- diversity and shifts in bacterial compositions at phylum and genus levels.
34277461	3	77	theme	cardiovascular	647:660	arg1	reduction					667:675	atherosclerotic cardiovascular risk reduction	631:675	atherosclerotic cardiovascular risk reduction	631:675	The association of CIH with the development of altered gut microbiota (GM) may provide the opportunity to develop preventive strategies for atherosclerotic cardiovascular risk reduction.
34277461	12	78	theme	pathological	1781:1792	arg1	changes					1794:1800	pathological changes	1781:1800	pathological changes in artery	1781:1810	The correlation of intestinal bacterial parameters with pathological changes in artery indicated complicated interactions under CIH-induced GM dysbiosis.
34277461	13	79	from	functions	1910:1918	arg1	ability					1937:1943	the potential ability	1923:1943	the potential ability of replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins	1923:2077	Furthermore, the gut microbial functions in the potential ability of replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins were identified to be further suppressed by CIH.
34277461	4	80	with	patients	842:849	arg1	OSA					856:858	OSA	856:858	OSA	856:858	Animal models of apolipoprotein E-deficient (apoE-/-) mice treated with high-fat diet (HFD) and subjected to CIH conditions was applied to mimic the AS observed in patients with OSA.
34277461	5	81	theme	histological	941:952	arg1	analysis					954:961	histological analysis	941:961	histological analysis	941:961	The physiological status and atherosclerotic lesion formation were confirmed by histological analysis.
34277461	12	82	theme	intestinal	1744:1753	arg1	parameters					1765:1774	intestinal bacterial parameters	1744:1774	intestinal bacterial parameters	1744:1774	The correlation of intestinal bacterial parameters with pathological changes in artery indicated complicated interactions under CIH-induced GM dysbiosis.
34277461	6	83	theme	microbial	1047:1055	arg1	composition					1057:1067	gut microbial composition	1043:1067	gut microbial composition	1043:1067	16S rDNA sequencing of fecal samples was conducted to determine the changes in gut microbial composition.
34277461	0	84	theme	Intestinal	111:120	arg1	Microbiota					122:131	Intestinal Microbiota	111:131	Intestinal Microbiota	111:131	Chronic Intermittent Hypoxia Participates in the Pathogenesis of Atherosclerosis and Perturbs the Formation of Intestinal Microbiota.
34277461	9	85	theme	AS	1366:1367	arg1	mice					1369:1372	AS mice	1366:1372	AS mice with and without CIH	1366:1393	Significantly perturbed GM profiles were detected in AS mice with and without CIH, with altered microbial α- and β- diversity and shifts in bacterial compositions at phylum and genus levels.
34277461	12	86	theme	parameters	1765:1774	arg1	correlation					1729:1739	The correlation	1725:1739	The correlation of intestinal bacterial parameters with pathological changes in artery	1725:1810	The correlation of intestinal bacterial parameters with pathological changes in artery indicated complicated interactions under CIH-induced GM dysbiosis.
34277461	4	87	theme	CIH	787:789	arg1	conditions					791:800	CIH conditions	787:800	CIH conditions	787:800	Animal models of apolipoprotein E-deficient (apoE-/-) mice treated with high-fat diet (HFD) and subjected to CIH conditions was applied to mimic the AS observed in patients with OSA.
34277461	1	88	theme	obstructive	216:226	arg1	OSA					241:243	OSA	241:243	OSA	241:243	Chronic intermittent hypoxia (CIH) is the prominent signature of highly prevalent obstructive sleep apnea (OSA) pathophysiology, which leads to increased risk and aggravation of atherosclerotic cardiovascular diseases.
34277461	1	88	theme	obstructive	216:226	arg1	apnea					234:238	obstructive sleep apnea	216:238	highly prevalent obstructive sleep apnea (OSA) pathophysiology	199:260	Chronic intermittent hypoxia (CIH) is the prominent signature of highly prevalent obstructive sleep apnea (OSA) pathophysiology, which leads to increased risk and aggravation of atherosclerotic cardiovascular diseases.
34277461	4	89	dep	mice	732:735	arg1	subjected					774:782	subjected	774:782	subjected to CIH conditions	774:800	Animal models of apolipoprotein E-deficient (apoE-/-) mice treated with high-fat diet (HFD) and subjected to CIH conditions was applied to mimic the AS observed in patients with OSA.
34277461	4	89	dep	mice	732:735	arg1	treated					737:743	treated	737:743	treated with high-fat diet (HFD)	737:768	Animal models of apolipoprotein E-deficient (apoE-/-) mice treated with high-fat diet (HFD) and subjected to CIH conditions was applied to mimic the AS observed in patients with OSA.
34277461	6	90	from	changes	1032:1038	arg1	composition					1057:1067	gut microbial composition	1043:1067	gut microbial composition	1043:1067	16S rDNA sequencing of fecal samples was conducted to determine the changes in gut microbial composition.
34277461	13	91	theme	repair	1978:1983	arg1	biosynthesis					2002:2013	glycan biosynthesis	1995:2013	glycan biosynthesis	1995:2013	Furthermore, the gut microbial functions in the potential ability of replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins were identified to be further suppressed by CIH.
34277461	13	91	theme	repair	1978:1983	arg1	metabolism					2019:2028	metabolism	2019:2028	metabolism	2019:2028	Furthermore, the gut microbial functions in the potential ability of replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins were identified to be further suppressed by CIH.
34277461	13	91	theme	repair	1978:1983	arg1	proteins					1985:1992	replication recombination and repair proteins	1948:1992	replication recombination and repair proteins	1948:1992	Furthermore, the gut microbial functions in the potential ability of replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins were identified to be further suppressed by CIH.
34277461	14	92	theme	GM	2180:2181	arg1	alterations					2183:2193	GM alterations	2180:2193	GM alterations in AS mice	2180:2204	Our findings demonstrated a causal effect of CIH on GM alterations in AS mice and suggested that the disordered GM features in AS development were deteriorated by CIH, which may be associated with AS aggravation.
34277461	13	93	theme	potential	1927:1935	arg1	ability					1937:1943	the potential ability	1923:1943	the potential ability of replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins	1923:2077	Furthermore, the gut microbial functions in the potential ability of replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins were identified to be further suppressed by CIH.
34277461	15	94	theme	gut	2375:2377	arg1	microbiome					2379:2388	gut microbiome	2375:2388	gut microbiome	2375:2388	Preventative strategies targeting gut microbiome are highly recommended for intervention of OSA-related AS.
34277461	14	95	theme	CIH	2173:2175	arg1	effect					2163:2168	a causal effect	2154:2168	a causal effect of CIH on GM alterations in AS mice	2154:2204	Our findings demonstrated a causal effect of CIH on GM alterations in AS mice and suggested that the disordered GM features in AS development were deteriorated by CIH, which may be associated with AS aggravation.
34277461	14	96	theme	AS	2325:2326	arg1	aggravation					2328:2338	AS aggravation	2325:2338	AS aggravation	2325:2338	Our findings demonstrated a causal effect of CIH on GM alterations in AS mice and suggested that the disordered GM features in AS development were deteriorated by CIH, which may be associated with AS aggravation.
34277461	2	97	theme	arteriosclerosis	439:454	arg1	pathogenesis					461:472	arteriosclerosis (AS) pathogenesis	439:472	arteriosclerosis (AS) pathogenesis	439:472	However, whether intestinal microbiota is implicated in the mechanisms linking CIH to arteriosclerosis (AS) pathogenesis remains unclear.
34277461	9	98	theme	altered	1401:1407	arg1	α-					1419:1420	altered microbial α-	1401:1420	altered microbial α-	1401:1420	Significantly perturbed GM profiles were detected in AS mice with and without CIH, with altered microbial α- and β- diversity and shifts in bacterial compositions at phylum and genus levels.
34277461	14	99	from	effect	2163:2168	arg1	alterations					2183:2193	GM alterations	2180:2193	GM alterations in AS mice	2180:2204	Our findings demonstrated a causal effect of CIH on GM alterations in AS mice and suggested that the disordered GM features in AS development were deteriorated by CIH, which may be associated with AS aggravation.
34277461	0	100	theme	Atherosclerosis	65:79	arg1	Pathogenesis					49:60	the Pathogenesis	45:60	the Pathogenesis of Atherosclerosis	45:79	Chronic Intermittent Hypoxia Participates in the Pathogenesis of Atherosclerosis and Perturbs the Formation of Intestinal Microbiota.
34277461	1	101	theme	atherosclerotic	312:326	arg1	diseases					343:350	atherosclerotic cardiovascular diseases	312:350	atherosclerotic cardiovascular diseases	312:350	Chronic intermittent hypoxia (CIH) is the prominent signature of highly prevalent obstructive sleep apnea (OSA) pathophysiology, which leads to increased risk and aggravation of atherosclerotic cardiovascular diseases.
34277461	9	102	from	shifts	1443:1448	arg1	compositions					1463:1474	bacterial compositions	1453:1474	bacterial compositions	1453:1474	Significantly perturbed GM profiles were detected in AS mice with and without CIH, with altered microbial α- and β- diversity and shifts in bacterial compositions at phylum and genus levels.
34277461	7	103	theme	atherosclerotic	1132:1146	arg1	lesions					1148:1154	aggravated atherosclerotic lesions	1121:1154	aggravated atherosclerotic lesions	1121:1154	Morphometric analysis demonstrated that CIH caused aggravated atherosclerotic lesions and facilitated AS in apoE-/- mice treated with HFD.
34277461	10	104	theme	different	1563:1571	arg1	levels					1587:1592	different bacteria taxa levels	1563:1592	different bacteria taxa levels	1563:1592	While the difference between AS and AS+CIH was observed at different bacteria taxa levels.
34277461	2	105	dep	arteriosclerosis	439:454	arg1	AS					457:458	AS	457:458	AS	457:458	However, whether intestinal microbiota is implicated in the mechanisms linking CIH to arteriosclerosis (AS) pathogenesis remains unclear.
34277461	11	106	theme	increased	1633:1641	arg1	Halomonas					1643:1651	increased Halomonas	1633:1651	increased Halomonas	1633:1651	Aggravation of reduced Sutterella and increased Halomonas, Halomonadaceae and Oceanospirillales was noted in CIH-treated AS mice.
34277461	9	107	theme	β-	1426:1427	arg1	diversity					1429:1437	β- diversity	1426:1437	β- diversity	1426:1437	Significantly perturbed GM profiles were detected in AS mice with and without CIH, with altered microbial α- and β- diversity and shifts in bacterial compositions at phylum and genus levels.
34277461	9	108	from	diversity	1429:1437	arg1	compositions					1463:1474	bacterial compositions	1453:1474	bacterial compositions	1453:1474	Significantly perturbed GM profiles were detected in AS mice with and without CIH, with altered microbial α- and β- diversity and shifts in bacterial compositions at phylum and genus levels.
34277461	8	109	from	varied	1244:1249	arg1	AS+CIH					1261:1266	AS+CIH	1261:1266	AS+CIH	1261:1266	The gut bacteria was significantly varied in AS and AS+CIH mice compared with that in the control mice.
34277461	8	109	from	varied	1244:1249	arg1	AS					1254:1255	AS	1254:1255	AS	1254:1255	The gut bacteria was significantly varied in AS and AS+CIH mice compared with that in the control mice.
34277461	8	110	dep	AS	1254:1255	arg1	mice					1268:1271	mice	1268:1271	mice	1268:1271	The gut bacteria was significantly varied in AS and AS+CIH mice compared with that in the control mice.
34277461	3	111	theme	CIH	510:512	arg1	association					495:505	The association	491:505	The association of CIH with the development of altered gut microbiota (GM)	491:564	The association of CIH with the development of altered gut microbiota (GM) may provide the opportunity to develop preventive strategies for atherosclerotic cardiovascular risk reduction.
34277461	4	112	theme	Animal	678:683	arg1	models					685:690	Animal models	678:690	Animal models	678:690	Animal models of apolipoprotein E-deficient (apoE-/-) mice treated with high-fat diet (HFD) and subjected to CIH conditions was applied to mimic the AS observed in patients with OSA.
34277461	13	113	dep	replication	1948:1958	arg1	recombination					1960:1972	recombination	1960:1972	recombination	1960:1972	Furthermore, the gut microbial functions in the potential ability of replication recombination and repair proteins, glycan biosynthesis and metabolism, as well as metabolism of cofactors and vitamins were identified to be further suppressed by CIH.
34277461	11	114	theme	AS	1716:1717	arg1	mice					1719:1722	CIH-treated AS mice	1704:1722	CIH-treated AS mice	1704:1722	Aggravation of reduced Sutterella and increased Halomonas, Halomonadaceae and Oceanospirillales was noted in CIH-treated AS mice.
34277461	3	115	theme	gut	546:548	arg1	GM					562:563	GM	562:563	GM	562:563	The association of CIH with the development of altered gut microbiota (GM) may provide the opportunity to develop preventive strategies for atherosclerotic cardiovascular risk reduction.
34277461	3	115	theme	gut	546:548	arg1	microbiota					550:559	altered gut microbiota	538:559	altered gut microbiota (GM)	538:564	The association of CIH with the development of altered gut microbiota (GM) may provide the opportunity to develop preventive strategies for atherosclerotic cardiovascular risk reduction.
34277461	1	116	theme	pathophysiology	246:260	arg1	hypoxia					155:161	Chronic intermittent hypoxia	134:161	Chronic intermittent hypoxia (CIH)	134:167	Chronic intermittent hypoxia (CIH) is the prominent signature of highly prevalent obstructive sleep apnea (OSA) pathophysiology, which leads to increased risk and aggravation of atherosclerotic cardiovascular diseases.
34277461	1	116	theme	pathophysiology	246:260	arg1	signature					186:194	the prominent signature	172:194	the prominent signature	172:194	Chronic intermittent hypoxia (CIH) is the prominent signature of highly prevalent obstructive sleep apnea (OSA) pathophysiology, which leads to increased risk and aggravation of atherosclerotic cardiovascular diseases.
34645930	4	0	theme	ROS	881:883	arg1	levels					871:876	the levels	867:876	the levels of ROS, lipid peroxidation, NO, and the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes	867:990	Also, the outcomes revealed an improvement in the CCl4-induced liver, lung, brain, and spleen toxicity by reducing the levels of ROS, lipid peroxidation, NO, and the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes.
34645930	4	1	dep	liver	815:819	arg1	toxicity					846:853	toxicity	846:853	toxicity	846:853	Also, the outcomes revealed an improvement in the CCl4-induced liver, lung, brain, and spleen toxicity by reducing the levels of ROS, lipid peroxidation, NO, and the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes.
34645930	4	2	theme	gene	918:921	arg1	expression					923:932	the gene expression	914:932	the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes	914:990	Also, the outcomes revealed an improvement in the CCl4-induced liver, lung, brain, and spleen toxicity by reducing the levels of ROS, lipid peroxidation, NO, and the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes.
34645930	2	3	theme	CCl4	428:431	arg1	administration					433:446	CCl4 administration	428:446	CCl4 administration	428:446	The present research evaluated the systemic oxidative and necroinflammatory stress induced by CCl4 administration and the alleviating effect of AS gum aqueous extract (ASE, 7.5 g/Kg b.w.).
34645930	4	4	from	improvement	783:793	arg1	brain					828:832	brain	828:832	brain	828:832	Also, the outcomes revealed an improvement in the CCl4-induced liver, lung, brain, and spleen toxicity by reducing the levels of ROS, lipid peroxidation, NO, and the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes.
34645930	4	4	from	improvement	783:793	arg1	spleen					839:844	spleen	839:844	spleen	839:844	Also, the outcomes revealed an improvement in the CCl4-induced liver, lung, brain, and spleen toxicity by reducing the levels of ROS, lipid peroxidation, NO, and the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes.
34645930	4	4	from	improvement	783:793	arg1	lung					822:825	lung	822:825	lung	822:825	Also, the outcomes revealed an improvement in the CCl4-induced liver, lung, brain, and spleen toxicity by reducing the levels of ROS, lipid peroxidation, NO, and the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes.
34645930	4	4	from	improvement	783:793	arg1	liver					815:819	the CCl4-induced liver	798:819	the CCl4-induced liver	798:819	Also, the outcomes revealed an improvement in the CCl4-induced liver, lung, brain, and spleen toxicity by reducing the levels of ROS, lipid peroxidation, NO, and the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes.
34645930	4	5	theme	NF-κB	937:941	arg1	peroxidation					892:903	lipid peroxidation	886:903	lipid peroxidation	886:903	Also, the outcomes revealed an improvement in the CCl4-induced liver, lung, brain, and spleen toxicity by reducing the levels of ROS, lipid peroxidation, NO, and the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes.
34645930	4	5	theme	NF-κB	937:941	arg1	expression					923:932	the gene expression	914:932	the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes	914:990	Also, the outcomes revealed an improvement in the CCl4-induced liver, lung, brain, and spleen toxicity by reducing the levels of ROS, lipid peroxidation, NO, and the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes.
34645930	4	5	theme	NF-κB	937:941	arg1	NO					906:907	NO	906:907	NO	906:907	Also, the outcomes revealed an improvement in the CCl4-induced liver, lung, brain, and spleen toxicity by reducing the levels of ROS, lipid peroxidation, NO, and the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes.
34645930	4	5	theme	NF-κB	937:941	arg1	ROS					881:883	ROS	881:883	ROS	881:883	Also, the outcomes revealed an improvement in the CCl4-induced liver, lung, brain, and spleen toxicity by reducing the levels of ROS, lipid peroxidation, NO, and the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes.
34645930	3	6	theme	compounds	581:589	arg1	ability					641:647	its in vitro potent scavenging ability	610:647	its in vitro potent scavenging ability	610:647	The results demonstrated the presence of certain phenolic compounds in ASE, as well as its in vitro potent scavenging ability against ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), NO, and lipid peroxide radicals.
34645930	3	6	theme	compounds	581:589	arg1	presence					552:559	the presence	548:559	the presence of certain phenolic compounds in ASE	548:596	The results demonstrated the presence of certain phenolic compounds in ASE, as well as its in vitro potent scavenging ability against ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), NO, and lipid peroxide radicals.
34645930	0	7	theme	ROS/NF-κB	95:103	arg1	pathway					115:121	the ROS/NF-κB signaling pathway	91:121	the ROS/NF-κB signaling pathway	91:121	Acacia senegal gum attenuates systemic toxicity in CCl4-intoxicated rats via regulation of the ROS/NF-κB signaling pathway.
34645930	5	8	theme	total	1010:1014	arg1	TAC					1038:1040	TAC	1038:1040	TAC	1038:1040	In contrast, the total antioxidant capacity (TAC), as well as the enzymatic and non-enzymatic antioxidants, were significantly upregulated in these organs after the treatment with ASE.
34645930	5	8	theme	total	1010:1014	arg1	capacity					1028:1035	the total antioxidant capacity	1006:1035	the total antioxidant capacity (TAC)	1006:1041	In contrast, the total antioxidant capacity (TAC), as well as the enzymatic and non-enzymatic antioxidants, were significantly upregulated in these organs after the treatment with ASE.
34645930	1	9	dep	senegal	131:137	arg1	AS					140:141	AS	140:141	AS	140:141	Acacia senegal (AS) gum (Gum Arabic) is a natural emulsifier exudate from the branches and trunk of Acacia trees and it is recognized by the Food and Drug Administration (FDA) agency as a secure dietary fiber.
34645930	1	10	theme	Acacia	224:229	arg1	trees					231:235	Acacia trees	224:235	Acacia trees	224:235	Acacia senegal (AS) gum (Gum Arabic) is a natural emulsifier exudate from the branches and trunk of Acacia trees and it is recognized by the Food and Drug Administration (FDA) agency as a secure dietary fiber.
34645930	6	11	theme	morphological	1224:1236	arg1	features					1238:1245	the morphological features	1220:1245	the morphological features of each organ	1220:1259	These results were confirmed by improving the morphological features of each organ.
34645930	1	12	dep	Food	265:268	arg1	agency					300:305	agency	300:305	agency	300:305	Acacia senegal (AS) gum (Gum Arabic) is a natural emulsifier exudate from the branches and trunk of Acacia trees and it is recognized by the Food and Drug Administration (FDA) agency as a secure dietary fiber.
34645930	1	12	dep	Food	265:268	arg1	the					261:263	the	261:263	the	261:263	Acacia senegal (AS) gum (Gum Arabic) is a natural emulsifier exudate from the branches and trunk of Acacia trees and it is recognized by the Food and Drug Administration (FDA) agency as a secure dietary fiber.
34645930	7	13	from	pathway	1371:1377	arg1	organs					1390:1395	the rat organs	1382:1395	the rat organs	1382:1395	Therefore, ASE can ameliorate the systemic toxicity caused by CCl4 via regulation of the ROS/NF-κB signaling pathway in the rat organs, which is owed to its phytochemical composition.
34645930	1	14	theme	trees	231:235	arg1	gum					144:146	Acacia senegal (AS) gum	124:146	Acacia senegal (AS) gum (Gum Arabic)	124:159	Acacia senegal (AS) gum (Gum Arabic) is a natural emulsifier exudate from the branches and trunk of Acacia trees and it is recognized by the Food and Drug Administration (FDA) agency as a secure dietary fiber.
34645930	1	14	theme	trees	231:235	arg1	trunk					215:219	trunk	215:219	trunk	215:219	Acacia senegal (AS) gum (Gum Arabic) is a natural emulsifier exudate from the branches and trunk of Acacia trees and it is recognized by the Food and Drug Administration (FDA) agency as a secure dietary fiber.
34645930	1	14	theme	trees	231:235	arg1	exudate					185:191	a natural emulsifier exudate	164:191	a natural emulsifier exudate from the branches	164:209	Acacia senegal (AS) gum (Gum Arabic) is a natural emulsifier exudate from the branches and trunk of Acacia trees and it is recognized by the Food and Drug Administration (FDA) agency as a secure dietary fiber.
34645930	0	15	theme	pathway	115:121	arg1	regulation					77:86	regulation	77:86	regulation of the ROS/NF-κB signaling pathway	77:121	Acacia senegal gum attenuates systemic toxicity in CCl4-intoxicated rats via regulation of the ROS/NF-κB signaling pathway.
34645930	5	16	with	treatment	1158:1166	arg1	ASE					1173:1175	ASE	1173:1175	ASE	1173:1175	In contrast, the total antioxidant capacity (TAC), as well as the enzymatic and non-enzymatic antioxidants, were significantly upregulated in these organs after the treatment with ASE.
34645930	1	17	theme	Gum	149:151	arg1	gum					144:146	Acacia senegal (AS) gum	124:146	Acacia senegal (AS) gum (Gum Arabic)	124:159	Acacia senegal (AS) gum (Gum Arabic) is a natural emulsifier exudate from the branches and trunk of Acacia trees and it is recognized by the Food and Drug Administration (FDA) agency as a secure dietary fiber.
34645930	1	17	theme	Gum	149:151	arg1	Arabic					153:158	Gum Arabic	149:158	Gum Arabic	149:158	Acacia senegal (AS) gum (Gum Arabic) is a natural emulsifier exudate from the branches and trunk of Acacia trees and it is recognized by the Food and Drug Administration (FDA) agency as a secure dietary fiber.
34645930	0	18	theme	signaling	105:113	arg1	pathway					115:121	the ROS/NF-κB signaling pathway	91:121	the ROS/NF-κB signaling pathway	91:121	Acacia senegal gum attenuates systemic toxicity in CCl4-intoxicated rats via regulation of the ROS/NF-κB signaling pathway.
34645930	0	19	from	toxicity	39:46	arg1	rats					68:71	CCl4-intoxicated rats	51:71	CCl4-intoxicated rats	51:71	Acacia senegal gum attenuates systemic toxicity in CCl4-intoxicated rats via regulation of the ROS/NF-κB signaling pathway.
34645930	7	20	from	regulation	1333:1342	arg1	organs					1390:1395	the rat organs	1382:1395	the rat organs	1382:1395	Therefore, ASE can ameliorate the systemic toxicity caused by CCl4 via regulation of the ROS/NF-κB signaling pathway in the rat organs, which is owed to its phytochemical composition.
34645930	2	21	theme	oxidative	378:386	arg1	stress					410:415	the systemic oxidative and necroinflammatory stress	365:415	the systemic oxidative and necroinflammatory stress induced by CCl4 administration	365:446	The present research evaluated the systemic oxidative and necroinflammatory stress induced by CCl4 administration and the alleviating effect of AS gum aqueous extract (ASE, 7.5 g/Kg b.w.).
34645930	3	22	from	presence	552:559	arg1	ASE					594:596	ASE	594:596	ASE	594:596	The results demonstrated the presence of certain phenolic compounds in ASE, as well as its in vitro potent scavenging ability against ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), NO, and lipid peroxide radicals.
34645930	2	23	dep	extract	493:499	arg1	ASE					502:504	ASE	502:504	ASE	502:504	The present research evaluated the systemic oxidative and necroinflammatory stress induced by CCl4 administration and the alleviating effect of AS gum aqueous extract (ASE, 7.5 g/Kg b.w.).
34645930	2	23	dep	extract	493:499	arg1	b.w.					516:519	7.5 g/Kg b.w.	507:519	7.5 g/Kg b.w.	507:519	The present research evaluated the systemic oxidative and necroinflammatory stress induced by CCl4 administration and the alleviating effect of AS gum aqueous extract (ASE, 7.5 g/Kg b.w.).
34645930	0	24	theme	senegal	7:13	arg1	gum					15:17	Acacia senegal gum	0:17	Acacia senegal gum	0:17	Acacia senegal gum attenuates systemic toxicity in CCl4-intoxicated rats via regulation of the ROS/NF-κB signaling pathway.
34645930	3	25	theme	scavenging	630:639	arg1	ability					641:647	its in vitro potent scavenging ability	610:647	its in vitro potent scavenging ability	610:647	The results demonstrated the presence of certain phenolic compounds in ASE, as well as its in vitro potent scavenging ability against ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), NO, and lipid peroxide radicals.
34645930	4	26	theme	CCl4-induced	802:813	arg1	liver					815:819	the CCl4-induced liver	798:819	the CCl4-induced liver	798:819	Also, the outcomes revealed an improvement in the CCl4-induced liver, lung, brain, and spleen toxicity by reducing the levels of ROS, lipid peroxidation, NO, and the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes.
34645930	0	27	theme	Acacia	0:5	arg1	gum					15:17	Acacia senegal gum	0:17	Acacia senegal gum	0:17	Acacia senegal gum attenuates systemic toxicity in CCl4-intoxicated rats via regulation of the ROS/NF-κB signaling pathway.
34645930	7	28	theme	ROS/NF-κB	1351:1359	arg1	pathway					1371:1377	the ROS/NF-κB signaling pathway	1347:1377	the ROS/NF-κB signaling pathway in the rat organs	1347:1395	Therefore, ASE can ameliorate the systemic toxicity caused by CCl4 via regulation of the ROS/NF-κB signaling pathway in the rat organs, which is owed to its phytochemical composition.
34645930	4	29	theme	NO	906:907	arg1	levels					871:876	the levels	867:876	the levels of ROS, lipid peroxidation, NO, and the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes	867:990	Also, the outcomes revealed an improvement in the CCl4-induced liver, lung, brain, and spleen toxicity by reducing the levels of ROS, lipid peroxidation, NO, and the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes.
34645930	4	30	theme	genes	986:990	arg1	peroxidation					892:903	lipid peroxidation	886:903	lipid peroxidation	886:903	Also, the outcomes revealed an improvement in the CCl4-induced liver, lung, brain, and spleen toxicity by reducing the levels of ROS, lipid peroxidation, NO, and the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes.
34645930	4	30	theme	genes	986:990	arg1	expression					923:932	the gene expression	914:932	the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes	914:990	Also, the outcomes revealed an improvement in the CCl4-induced liver, lung, brain, and spleen toxicity by reducing the levels of ROS, lipid peroxidation, NO, and the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes.
34645930	4	30	theme	genes	986:990	arg1	NO					906:907	NO	906:907	NO	906:907	Also, the outcomes revealed an improvement in the CCl4-induced liver, lung, brain, and spleen toxicity by reducing the levels of ROS, lipid peroxidation, NO, and the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes.
34645930	4	30	theme	genes	986:990	arg1	ROS					881:883	ROS	881:883	ROS	881:883	Also, the outcomes revealed an improvement in the CCl4-induced liver, lung, brain, and spleen toxicity by reducing the levels of ROS, lipid peroxidation, NO, and the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes.
34645930	2	31	theme	systemic	369:376	arg1	stress					410:415	the systemic oxidative and necroinflammatory stress	365:415	the systemic oxidative and necroinflammatory stress induced by CCl4 administration	365:446	The present research evaluated the systemic oxidative and necroinflammatory stress induced by CCl4 administration and the alleviating effect of AS gum aqueous extract (ASE, 7.5 g/Kg b.w.).
34645930	4	32	theme	inflammatory	973:984	arg1	genes					986:990	its relevant ROS-mediated inflammatory genes	947:990	its relevant ROS-mediated inflammatory genes	947:990	Also, the outcomes revealed an improvement in the CCl4-induced liver, lung, brain, and spleen toxicity by reducing the levels of ROS, lipid peroxidation, NO, and the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes.
34645930	3	33	theme	in	614:615	arg1	ability					641:647	its in vitro potent scavenging ability	610:647	its in vitro potent scavenging ability	610:647	The results demonstrated the presence of certain phenolic compounds in ASE, as well as its in vitro potent scavenging ability against ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), NO, and lipid peroxide radicals.
34645930	3	34	dep	in	614:615	arg1	vitro					617:621	vitro	617:621	vitro	617:621	The results demonstrated the presence of certain phenolic compounds in ASE, as well as its in vitro potent scavenging ability against ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), NO, and lipid peroxide radicals.
34645930	2	35	theme	extract	493:499	arg1	stress					410:415	the systemic oxidative and necroinflammatory stress	365:415	the systemic oxidative and necroinflammatory stress induced by CCl4 administration	365:446	The present research evaluated the systemic oxidative and necroinflammatory stress induced by CCl4 administration and the alleviating effect of AS gum aqueous extract (ASE, 7.5 g/Kg b.w.).
34645930	2	35	theme	extract	493:499	arg1	effect					468:473	the alleviating effect	452:473	the alleviating effect of AS gum aqueous extract (ASE, 7.5 g/Kg b.w.)	452:520	The present research evaluated the systemic oxidative and necroinflammatory stress induced by CCl4 administration and the alleviating effect of AS gum aqueous extract (ASE, 7.5 g/Kg b.w.).
34645930	1	36	theme	secure	312:317	arg1	fiber					327:331	a secure dietary fiber	310:331	a secure dietary fiber	310:331	Acacia senegal (AS) gum (Gum Arabic) is a natural emulsifier exudate from the branches and trunk of Acacia trees and it is recognized by the Food and Drug Administration (FDA) agency as a secure dietary fiber.
34645930	1	36	theme	secure	312:317	arg1	it					241:242	it	241:242	it	241:242	Acacia senegal (AS) gum (Gum Arabic) is a natural emulsifier exudate from the branches and trunk of Acacia trees and it is recognized by the Food and Drug Administration (FDA) agency as a secure dietary fiber.
34645930	1	37	theme	natural	166:172	arg1	gum					144:146	Acacia senegal (AS) gum	124:146	Acacia senegal (AS) gum (Gum Arabic)	124:159	Acacia senegal (AS) gum (Gum Arabic) is a natural emulsifier exudate from the branches and trunk of Acacia trees and it is recognized by the Food and Drug Administration (FDA) agency as a secure dietary fiber.
34645930	1	37	theme	natural	166:172	arg1	exudate					185:191	a natural emulsifier exudate	164:191	a natural emulsifier exudate from the branches	164:209	Acacia senegal (AS) gum (Gum Arabic) is a natural emulsifier exudate from the branches and trunk of Acacia trees and it is recognized by the Food and Drug Administration (FDA) agency as a secure dietary fiber.
34645930	4	38	theme	lipid	886:890	arg1	peroxidation					892:903	lipid peroxidation	886:903	lipid peroxidation	886:903	Also, the outcomes revealed an improvement in the CCl4-induced liver, lung, brain, and spleen toxicity by reducing the levels of ROS, lipid peroxidation, NO, and the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes.
34645930	2	39	theme	aqueous	485:491	arg1	extract					493:499	AS gum aqueous extract	478:499	AS gum aqueous extract (ASE, 7.5 g/Kg b.w.)	478:520	The present research evaluated the systemic oxidative and necroinflammatory stress induced by CCl4 administration and the alleviating effect of AS gum aqueous extract (ASE, 7.5 g/Kg b.w.).
34645930	1	40	theme	dietary	319:325	arg1	fiber					327:331	a secure dietary fiber	310:331	a secure dietary fiber	310:331	Acacia senegal (AS) gum (Gum Arabic) is a natural emulsifier exudate from the branches and trunk of Acacia trees and it is recognized by the Food and Drug Administration (FDA) agency as a secure dietary fiber.
34645930	1	40	theme	dietary	319:325	arg1	it					241:242	it	241:242	it	241:242	Acacia senegal (AS) gum (Gum Arabic) is a natural emulsifier exudate from the branches and trunk of Acacia trees and it is recognized by the Food and Drug Administration (FDA) agency as a secure dietary fiber.
34645930	1	41	theme	emulsifier	174:183	arg1	gum					144:146	Acacia senegal (AS) gum	124:146	Acacia senegal (AS) gum (Gum Arabic)	124:159	Acacia senegal (AS) gum (Gum Arabic) is a natural emulsifier exudate from the branches and trunk of Acacia trees and it is recognized by the Food and Drug Administration (FDA) agency as a secure dietary fiber.
34645930	1	41	theme	emulsifier	174:183	arg1	exudate					185:191	a natural emulsifier exudate	164:191	a natural emulsifier exudate from the branches	164:209	Acacia senegal (AS) gum (Gum Arabic) is a natural emulsifier exudate from the branches and trunk of Acacia trees and it is recognized by the Food and Drug Administration (FDA) agency as a secure dietary fiber.
34645930	0	42	theme	systemic	30:37	arg1	toxicity					39:46	systemic toxicity	30:46	systemic toxicity in CCl4-intoxicated rats	30:71	Acacia senegal gum attenuates systemic toxicity in CCl4-intoxicated rats via regulation of the ROS/NF-κB signaling pathway.
34645930	4	43	theme	peroxidation	892:903	arg1	levels					871:876	the levels	867:876	the levels of ROS, lipid peroxidation, NO, and the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes	867:990	Also, the outcomes revealed an improvement in the CCl4-induced liver, lung, brain, and spleen toxicity by reducing the levels of ROS, lipid peroxidation, NO, and the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes.
34645930	2	44	theme	gum	481:483	arg1	extract					493:499	AS gum aqueous extract	478:499	AS gum aqueous extract (ASE, 7.5 g/Kg b.w.)	478:520	The present research evaluated the systemic oxidative and necroinflammatory stress induced by CCl4 administration and the alleviating effect of AS gum aqueous extract (ASE, 7.5 g/Kg b.w.).
34645930	3	45	theme	certain	564:570	arg1	compounds					581:589	certain phenolic compounds	564:589	certain phenolic compounds	564:589	The results demonstrated the presence of certain phenolic compounds in ASE, as well as its in vitro potent scavenging ability against ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), NO, and lipid peroxide radicals.
34645930	3	46	from	ability	641:647	arg1	ASE					594:596	ASE	594:596	ASE	594:596	The results demonstrated the presence of certain phenolic compounds in ASE, as well as its in vitro potent scavenging ability against ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), NO, and lipid peroxide radicals.
34645930	2	47	theme	alleviating	456:466	arg1	effect					468:473	the alleviating effect	452:473	the alleviating effect of AS gum aqueous extract (ASE, 7.5 g/Kg b.w.)	452:520	The present research evaluated the systemic oxidative and necroinflammatory stress induced by CCl4 administration and the alleviating effect of AS gum aqueous extract (ASE, 7.5 g/Kg b.w.).
34645930	0	48	theme	CCl4-intoxicated	51:66	arg1	rats					68:71	CCl4-intoxicated rats	51:71	CCl4-intoxicated rats	51:71	Acacia senegal gum attenuates systemic toxicity in CCl4-intoxicated rats via regulation of the ROS/NF-κB signaling pathway.
34645930	2	49	theme	AS	478:479	arg1	extract					493:499	AS gum aqueous extract	478:499	AS gum aqueous extract (ASE, 7.5 g/Kg b.w.)	478:520	The present research evaluated the systemic oxidative and necroinflammatory stress induced by CCl4 administration and the alleviating effect of AS gum aqueous extract (ASE, 7.5 g/Kg b.w.).
34645930	1	50	from	branches	202:209	arg1	gum					144:146	Acacia senegal (AS) gum	124:146	Acacia senegal (AS) gum (Gum Arabic)	124:159	Acacia senegal (AS) gum (Gum Arabic) is a natural emulsifier exudate from the branches and trunk of Acacia trees and it is recognized by the Food and Drug Administration (FDA) agency as a secure dietary fiber.
34645930	1	50	from	branches	202:209	arg1	trunk					215:219	trunk	215:219	trunk	215:219	Acacia senegal (AS) gum (Gum Arabic) is a natural emulsifier exudate from the branches and trunk of Acacia trees and it is recognized by the Food and Drug Administration (FDA) agency as a secure dietary fiber.
34645930	1	50	from	branches	202:209	arg1	exudate					185:191	a natural emulsifier exudate	164:191	a natural emulsifier exudate from the branches	164:209	Acacia senegal (AS) gum (Gum Arabic) is a natural emulsifier exudate from the branches and trunk of Acacia trees and it is recognized by the Food and Drug Administration (FDA) agency as a secure dietary fiber.
34645930	3	51	theme	phenolic	572:579	arg1	compounds					581:589	certain phenolic compounds	564:589	certain phenolic compounds	564:589	The results demonstrated the presence of certain phenolic compounds in ASE, as well as its in vitro potent scavenging ability against ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), NO, and lipid peroxide radicals.
34645930	3	52	theme	potent	623:628	arg1	ability					641:647	its in vitro potent scavenging ability	610:647	its in vitro potent scavenging ability	610:647	The results demonstrated the presence of certain phenolic compounds in ASE, as well as its in vitro potent scavenging ability against ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), NO, and lipid peroxide radicals.
34645930	2	53	theme	necroinflammatory	392:408	arg1	stress					410:415	the systemic oxidative and necroinflammatory stress	365:415	the systemic oxidative and necroinflammatory stress induced by CCl4 administration	365:446	The present research evaluated the systemic oxidative and necroinflammatory stress induced by CCl4 administration and the alleviating effect of AS gum aqueous extract (ASE, 7.5 g/Kg b.w.).
34645930	3	54	theme	3-ethylbenzothiazoline-6-sulfonic	678:710	arg1	2,2'-azino-bis					663:676	2,2'-azino-bis	663:676	2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)	663:716	The results demonstrated the presence of certain phenolic compounds in ASE, as well as its in vitro potent scavenging ability against ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), NO, and lipid peroxide radicals.
34645930	3	54	theme	3-ethylbenzothiazoline-6-sulfonic	678:710	arg1	acid					712:715	3-ethylbenzothiazoline-6-sulfonic acid	678:715	3-ethylbenzothiazoline-6-sulfonic acid	678:715	The results demonstrated the presence of certain phenolic compounds in ASE, as well as its in vitro potent scavenging ability against ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), NO, and lipid peroxide radicals.
34645930	2	55	theme	7.5 g/Kg	507:514	arg1	ASE					502:504	ASE	502:504	ASE	502:504	The present research evaluated the systemic oxidative and necroinflammatory stress induced by CCl4 administration and the alleviating effect of AS gum aqueous extract (ASE, 7.5 g/Kg b.w.).
34645930	2	55	theme	7.5 g/Kg	507:514	arg1	b.w.					516:519	7.5 g/Kg b.w.	507:519	7.5 g/Kg b.w.	507:519	The present research evaluated the systemic oxidative and necroinflammatory stress induced by CCl4 administration and the alleviating effect of AS gum aqueous extract (ASE, 7.5 g/Kg b.w.).
34645930	2	56	theme	present	338:344	arg1	research					346:353	The present research	334:353	The present research	334:353	The present research evaluated the systemic oxidative and necroinflammatory stress induced by CCl4 administration and the alleviating effect of AS gum aqueous extract (ASE, 7.5 g/Kg b.w.).
34645930	5	57	theme	antioxidant	1016:1026	arg1	TAC					1038:1040	TAC	1038:1040	TAC	1038:1040	In contrast, the total antioxidant capacity (TAC), as well as the enzymatic and non-enzymatic antioxidants, were significantly upregulated in these organs after the treatment with ASE.
34645930	5	57	theme	antioxidant	1016:1026	arg1	capacity					1028:1035	the total antioxidant capacity	1006:1035	the total antioxidant capacity (TAC)	1006:1041	In contrast, the total antioxidant capacity (TAC), as well as the enzymatic and non-enzymatic antioxidants, were significantly upregulated in these organs after the treatment with ASE.
34645930	1	58	theme	Acacia	124:129	arg1	senegal					131:137	Acacia senegal	124:137	Acacia senegal (AS) gum (Gum Arabic)	124:159	Acacia senegal (AS) gum (Gum Arabic) is a natural emulsifier exudate from the branches and trunk of Acacia trees and it is recognized by the Food and Drug Administration (FDA) agency as a secure dietary fiber.
34645930	5	59	theme	enzymatic	1059:1067	arg1	antioxidants					1087:1098	the enzymatic and non-enzymatic antioxidants	1055:1098	the enzymatic and non-enzymatic antioxidants	1055:1098	In contrast, the total antioxidant capacity (TAC), as well as the enzymatic and non-enzymatic antioxidants, were significantly upregulated in these organs after the treatment with ASE.
34645930	1	60	theme	senegal	131:137	arg1	gum					144:146	Acacia senegal (AS) gum	124:146	Acacia senegal (AS) gum (Gum Arabic)	124:159	Acacia senegal (AS) gum (Gum Arabic) is a natural emulsifier exudate from the branches and trunk of Acacia trees and it is recognized by the Food and Drug Administration (FDA) agency as a secure dietary fiber.
34645930	1	60	theme	senegal	131:137	arg1	Arabic					153:158	Gum Arabic	149:158	Gum Arabic	149:158	Acacia senegal (AS) gum (Gum Arabic) is a natural emulsifier exudate from the branches and trunk of Acacia trees and it is recognized by the Food and Drug Administration (FDA) agency as a secure dietary fiber.
34645930	1	60	theme	senegal	131:137	arg1	exudate					185:191	a natural emulsifier exudate	164:191	a natural emulsifier exudate from the branches	164:209	Acacia senegal (AS) gum (Gum Arabic) is a natural emulsifier exudate from the branches and trunk of Acacia trees and it is recognized by the Food and Drug Administration (FDA) agency as a secure dietary fiber.
34645930	3	61	attach	presence	552:559	arg1	ASE					594:596	ASE	594:596	ASE	594:596	The results demonstrated the presence of certain phenolic compounds in ASE, as well as its in vitro potent scavenging ability against ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), NO, and lipid peroxide radicals.
34645930	3	61	attach	presence	552:559	arg2	compounds					581:589	certain phenolic compounds	564:589	certain phenolic compounds	564:589	The results demonstrated the presence of certain phenolic compounds in ASE, as well as its in vitro potent scavenging ability against ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), NO, and lipid peroxide radicals.
34645930	5	62	theme	non-enzymatic	1073:1085	arg1	antioxidants					1087:1098	the enzymatic and non-enzymatic antioxidants	1055:1098	the enzymatic and non-enzymatic antioxidants	1055:1098	In contrast, the total antioxidant capacity (TAC), as well as the enzymatic and non-enzymatic antioxidants, were significantly upregulated in these organs after the treatment with ASE.
34645930	7	63	theme	signaling	1361:1369	arg1	pathway					1371:1377	the ROS/NF-κB signaling pathway	1347:1377	the ROS/NF-κB signaling pathway in the rat organs	1347:1395	Therefore, ASE can ameliorate the systemic toxicity caused by CCl4 via regulation of the ROS/NF-κB signaling pathway in the rat organs, which is owed to its phytochemical composition.
34645930	7	64	theme	systemic	1296:1303	arg1	toxicity					1305:1312	the systemic toxicity	1292:1312	the systemic toxicity caused by CCl4	1292:1327	Therefore, ASE can ameliorate the systemic toxicity caused by CCl4 via regulation of the ROS/NF-κB signaling pathway in the rat organs, which is owed to its phytochemical composition.
34645930	4	65	theme	ROS-mediated	960:971	arg1	genes					986:990	its relevant ROS-mediated inflammatory genes	947:990	its relevant ROS-mediated inflammatory genes	947:990	Also, the outcomes revealed an improvement in the CCl4-induced liver, lung, brain, and spleen toxicity by reducing the levels of ROS, lipid peroxidation, NO, and the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes.
34645930	3	66	theme	lipid	727:731	arg1	radicals					742:749	lipid peroxide radicals	727:749	lipid peroxide radicals	727:749	The results demonstrated the presence of certain phenolic compounds in ASE, as well as its in vitro potent scavenging ability against ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), NO, and lipid peroxide radicals.
34645930	7	67	theme	pathway	1371:1377	arg1	regulation					1333:1342	regulation	1333:1342	regulation	1333:1342	Therefore, ASE can ameliorate the systemic toxicity caused by CCl4 via regulation of the ROS/NF-κB signaling pathway in the rat organs, which is owed to its phytochemical composition.
34645930	6	68	theme	organ	1255:1259	arg1	features					1238:1245	the morphological features	1220:1245	the morphological features of each organ	1220:1259	These results were confirmed by improving the morphological features of each organ.
34645930	4	69	theme	relevant	951:958	arg1	genes					986:990	its relevant ROS-mediated inflammatory genes	947:990	its relevant ROS-mediated inflammatory genes	947:990	Also, the outcomes revealed an improvement in the CCl4-induced liver, lung, brain, and spleen toxicity by reducing the levels of ROS, lipid peroxidation, NO, and the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes.
34645930	7	70	theme	phytochemical	1419:1431	arg1	composition					1433:1443	its phytochemical composition	1415:1443	its phytochemical composition	1415:1443	Therefore, ASE can ameliorate the systemic toxicity caused by CCl4 via regulation of the ROS/NF-κB signaling pathway in the rat organs, which is owed to its phytochemical composition.
34645930	3	71	theme	peroxide	733:740	arg1	radicals					742:749	lipid peroxide radicals	727:749	lipid peroxide radicals	727:749	The results demonstrated the presence of certain phenolic compounds in ASE, as well as its in vitro potent scavenging ability against ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), NO, and lipid peroxide radicals.
34645930	1	72	theme	Drug	274:277	arg1	FDA					295:297	FDA	295:297	FDA	295:297	Acacia senegal (AS) gum (Gum Arabic) is a natural emulsifier exudate from the branches and trunk of Acacia trees and it is recognized by the Food and Drug Administration (FDA) agency as a secure dietary fiber.
34645930	1	72	theme	Drug	274:277	arg1	Administration					279:292	Drug Administration	274:292	Drug Administration (FDA)	274:298	Acacia senegal (AS) gum (Gum Arabic) is a natural emulsifier exudate from the branches and trunk of Acacia trees and it is recognized by the Food and Drug Administration (FDA) agency as a secure dietary fiber.
34645930	7	73	theme	rat	1386:1388	arg1	organs					1390:1395	the rat organs	1382:1395	the rat organs	1382:1395	Therefore, ASE can ameliorate the systemic toxicity caused by CCl4 via regulation of the ROS/NF-κB signaling pathway in the rat organs, which is owed to its phytochemical composition.
34645930	4	74	theme	expression	923:932	arg1	levels					871:876	the levels	867:876	the levels of ROS, lipid peroxidation, NO, and the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes	867:990	Also, the outcomes revealed an improvement in the CCl4-induced liver, lung, brain, and spleen toxicity by reducing the levels of ROS, lipid peroxidation, NO, and the gene expression of NF-κB and its relevant ROS-mediated inflammatory genes.
34645930	7	75	from	organs	1390:1395	arg1	regulation					1333:1342	regulation	1333:1342	regulation	1333:1342	Therefore, ASE can ameliorate the systemic toxicity caused by CCl4 via regulation of the ROS/NF-κB signaling pathway in the rat organs, which is owed to its phytochemical composition.
32195245	0	0	from	N-Glycosylation	14:28	arg1	Mice					48:51	FcγR Knock-Out Mice	33:51	FcγR Knock-Out Mice	33:51	Fc-Linked IgG N-Glycosylation in FcγR Knock-Out Mice.
32195245	6	1	theme	stronger	957:964	arg1	effect					966:971	a stronger effect	955:971	a stronger effect on N-glycosylation of IgG Fc regions	955:1008	We observed slight changes in IgG Fc N-glycan profiles in different knock-outs; however, it seems that the strain background and sex have a stronger effect on N-glycosylation of IgG Fc regions than the FcγR repertoire.
32195245	6	2	theme	regions	1002:1008	arg1	effect					966:971	a stronger effect	955:971	a stronger effect on N-glycosylation of IgG Fc regions	955:1008	We observed slight changes in IgG Fc N-glycan profiles in different knock-outs; however, it seems that the strain background and sex have a stronger effect on N-glycosylation of IgG Fc regions than the FcγR repertoire.
32195245	6	3	theme	slight	829:834	arg1	changes					836:842	slight changes	829:842	slight changes in IgG Fc N-glycan profiles in different knock-outs	829:894	We observed slight changes in IgG Fc N-glycan profiles in different knock-outs; however, it seems that the strain background and sex have a stronger effect on N-glycosylation of IgG Fc regions than the FcγR repertoire.
32195245	5	4	theme	subclass-specific	696:712	arg1	glycoprofiles					721:733	the subclass-specific Fc IgG glycoprofiles	692:733	the subclass-specific Fc IgG glycoprofiles of healthy male and female FcγR knock-out mice on C57BL/6 and BALB/c backgrounds	692:814	To explore this, we examined the subclass-specific Fc IgG glycoprofiles of healthy male and female FcγR knock-out mice on C57BL/6 and BALB/c backgrounds.
32195245	5	5	theme	Fc	714:715	arg1	glycoprofiles					721:733	the subclass-specific Fc IgG glycoprofiles	692:733	the subclass-specific Fc IgG glycoprofiles of healthy male and female FcγR knock-out mice on C57BL/6 and BALB/c backgrounds	692:814	To explore this, we examined the subclass-specific Fc IgG glycoprofiles of healthy male and female FcγR knock-out mice on C57BL/6 and BALB/c backgrounds.
32195245	2	6	theme	pathological	316:327	arg1	states					329:334	different physiological and pathological states	288:334	different physiological and pathological states	288:334	Glycosylation of the IgG fragment crystallizable (Fc) region is shown to vary in different physiological and pathological states.
32195245	2	7	theme	fragment	232:239	arg1	region					261:266	the IgG fragment crystallizable (Fc) region	224:266	the IgG fragment crystallizable (Fc) region	224:266	Glycosylation of the IgG fragment crystallizable (Fc) region is shown to vary in different physiological and pathological states.
32195245	1	8	theme	Immunoglobulin	54:67	arg1	IgG					72:74	IgG	72:74	IgG	72:74	Immunoglobulin G (IgG) is the most abundant immunoglobulin isotype in the blood and is involved in the pathogenesis and progression of various diseases.
32195245	1	8	theme	Immunoglobulin	54:67	arg1	G					69:69	Immunoglobulin G	54:69	Immunoglobulin G (IgG)	54:75	Immunoglobulin G (IgG) is the most abundant immunoglobulin isotype in the blood and is involved in the pathogenesis and progression of various diseases.
32195245	3	9	theme	Fc	337:338	arg1	composition					349:359	Fc N-glycan composition	337:359	Fc N-glycan composition	337:359	Fc N-glycan composition can alter the effector functions of IgG by modulating its affinity for ligands, such as Fcγ receptors (FcγRs).
32195245	6	10	contain	have	950:953	arg1	background					931:940	the strain background	920:940	the strain background	920:940	We observed slight changes in IgG Fc N-glycan profiles in different knock-outs; however, it seems that the strain background and sex have a stronger effect on N-glycosylation of IgG Fc regions than the FcγR repertoire.
32195245	6	10	contain	have	950:953	arg2	effect					966:971	a stronger effect	955:971	a stronger effect on N-glycosylation of IgG Fc regions	955:1008	We observed slight changes in IgG Fc N-glycan profiles in different knock-outs; however, it seems that the strain background and sex have a stronger effect on N-glycosylation of IgG Fc regions than the FcγR repertoire.
32195245	6	10	contain	have	950:953	arg1	sex					946:948	sex	946:948	sex	946:948	We observed slight changes in IgG Fc N-glycan profiles in different knock-outs; however, it seems that the strain background and sex have a stronger effect on N-glycosylation of IgG Fc regions than the FcγR repertoire.
32195245	5	11	theme	C57BL/6	785:791	arg1	backgrounds					804:814	C57BL/6 and BALB/c backgrounds	785:814	C57BL/6 and BALB/c backgrounds	785:814	To explore this, we examined the subclass-specific Fc IgG glycoprofiles of healthy male and female FcγR knock-out mice on C57BL/6 and BALB/c backgrounds.
32195245	1	12	theme	abundant	89:96	arg1	immunoglobulin					98:111	the most abundant immunoglobulin	80:111	the most abundant immunoglobulin isotype in the blood	80:132	Immunoglobulin G (IgG) is the most abundant immunoglobulin isotype in the blood and is involved in the pathogenesis and progression of various diseases.
32195245	5	13	theme	knock-out	767:775	arg1	mice					777:780	healthy male and female FcγR knock-out mice	738:780	healthy male and female FcγR knock-out mice	738:780	To explore this, we examined the subclass-specific Fc IgG glycoprofiles of healthy male and female FcγR knock-out mice on C57BL/6 and BALB/c backgrounds.
32195245	2	14	theme	Fc	257:258	arg1	region					261:266	the IgG fragment crystallizable (Fc) region	224:266	the IgG fragment crystallizable (Fc) region	224:266	Glycosylation of the IgG fragment crystallizable (Fc) region is shown to vary in different physiological and pathological states.
32195245	5	15	theme	BALB/c	797:802	arg1	backgrounds					804:814	C57BL/6 and BALB/c backgrounds	785:814	C57BL/6 and BALB/c backgrounds	785:814	To explore this, we examined the subclass-specific Fc IgG glycoprofiles of healthy male and female FcγR knock-out mice on C57BL/6 and BALB/c backgrounds.
32195245	4	16	theme	Fc-linked	584:592	arg1	N-glycome					594:602	the Fc-linked N-glycome	580:602	the Fc-linked N-glycome	580:602	However, it is not known whether IgG glycosylation is affected by the available repertoire of FcγRs, and if the Fc-linked N-glycome can compensate for modulation of the IgG-FcγR interaction.
32195245	5	17	theme	healthy	738:744	arg1	mice					777:780	healthy male and female FcγR knock-out mice	738:780	healthy male and female FcγR knock-out mice	738:780	To explore this, we examined the subclass-specific Fc IgG glycoprofiles of healthy male and female FcγR knock-out mice on C57BL/6 and BALB/c backgrounds.
32195245	0	18	theme	Knock-Out	38:46	arg1	Mice					48:51	FcγR Knock-Out Mice	33:51	FcγR Knock-Out Mice	33:51	Fc-Linked IgG N-Glycosylation in FcγR Knock-Out Mice.
32195245	1	19	from	isotype	113:119	arg1	blood					128:132	the blood	124:132	the blood	124:132	Immunoglobulin G (IgG) is the most abundant immunoglobulin isotype in the blood and is involved in the pathogenesis and progression of various diseases.
32195245	5	20	theme	male	746:749	arg1	mice					777:780	healthy male and female FcγR knock-out mice	738:780	healthy male and female FcγR knock-out mice	738:780	To explore this, we examined the subclass-specific Fc IgG glycoprofiles of healthy male and female FcγR knock-out mice on C57BL/6 and BALB/c backgrounds.
32195245	0	21	theme	IgG	10:12	arg1	N-Glycosylation					14:28	Fc-Linked IgG N-Glycosylation	0:28	Fc-Linked IgG N-Glycosylation in FcγR Knock-Out Mice	0:51	Fc-Linked IgG N-Glycosylation in FcγR Knock-Out Mice.
32195245	6	22	theme	IgG	995:997	arg1	regions					1002:1008	IgG Fc regions	995:1008	IgG Fc regions	995:1008	We observed slight changes in IgG Fc N-glycan profiles in different knock-outs; however, it seems that the strain background and sex have a stronger effect on N-glycosylation of IgG Fc regions than the FcγR repertoire.
32195245	3	23	theme	IgG	397:399	arg1	functions					384:392	the effector functions	371:392	the effector functions of IgG	371:399	Fc N-glycan composition can alter the effector functions of IgG by modulating its affinity for ligands, such as Fcγ receptors (FcγRs).
32195245	0	24	theme	Fc-Linked	0:8	arg1	N-Glycosylation					14:28	Fc-Linked IgG N-Glycosylation	0:28	Fc-Linked IgG N-Glycosylation in FcγR Knock-Out Mice	0:51	Fc-Linked IgG N-Glycosylation in FcγR Knock-Out Mice.
32195245	6	25	from	effect	966:971	arg1	N-glycosylation					976:990	N-glycosylation	976:990	N-glycosylation	976:990	We observed slight changes in IgG Fc N-glycan profiles in different knock-outs; however, it seems that the strain background and sex have a stronger effect on N-glycosylation of IgG Fc regions than the FcγR repertoire.
32195245	3	26	theme	N-glycan	340:347	arg1	composition					349:359	Fc N-glycan composition	337:359	Fc N-glycan composition	337:359	Fc N-glycan composition can alter the effector functions of IgG by modulating its affinity for ligands, such as Fcγ receptors (FcγRs).
32195245	5	27	theme	FcγR	762:765	arg1	mice					777:780	healthy male and female FcγR knock-out mice	738:780	healthy male and female FcγR knock-out mice	738:780	To explore this, we examined the subclass-specific Fc IgG glycoprofiles of healthy male and female FcγR knock-out mice on C57BL/6 and BALB/c backgrounds.
32195245	5	28	theme	female	755:760	arg1	mice					777:780	healthy male and female FcγR knock-out mice	738:780	healthy male and female FcγR knock-out mice	738:780	To explore this, we examined the subclass-specific Fc IgG glycoprofiles of healthy male and female FcγR knock-out mice on C57BL/6 and BALB/c backgrounds.
32195245	4	29	theme	IgG	505:507	arg1	glycosylation					509:521	IgG glycosylation	505:521	IgG glycosylation	505:521	However, it is not known whether IgG glycosylation is affected by the available repertoire of FcγRs, and if the Fc-linked N-glycome can compensate for modulation of the IgG-FcγR interaction.
32195245	6	30	theme	Fc	999:1000	arg1	regions					1002:1008	IgG Fc regions	995:1008	IgG Fc regions	995:1008	We observed slight changes in IgG Fc N-glycan profiles in different knock-outs; however, it seems that the strain background and sex have a stronger effect on N-glycosylation of IgG Fc regions than the FcγR repertoire.
32195245	6	31	theme	IgG	847:849	arg1	profiles					863:870	IgG Fc N-glycan profiles	847:870	IgG Fc N-glycan profiles in different knock-outs	847:894	We observed slight changes in IgG Fc N-glycan profiles in different knock-outs; however, it seems that the strain background and sex have a stronger effect on N-glycosylation of IgG Fc regions than the FcγR repertoire.
32195245	4	32	theme	FcγRs	566:570	arg1	FcγRs					566:570	FcγRs	566:570	FcγRs	566:570	However, it is not known whether IgG glycosylation is affected by the available repertoire of FcγRs, and if the Fc-linked N-glycome can compensate for modulation of the IgG-FcγR interaction.
32195245	4	32	theme	FcγRs	566:570	arg1	repertoire					552:561	the available repertoire	538:561	the available repertoire of FcγRs	538:570	However, it is not known whether IgG glycosylation is affected by the available repertoire of FcγRs, and if the Fc-linked N-glycome can compensate for modulation of the IgG-FcγR interaction.
32195245	2	33	gly	Glycosylation	207:219	arg1	region					261:266	the IgG fragment crystallizable (Fc) region	224:266	the IgG fragment crystallizable (Fc) region	224:266	Glycosylation of the IgG fragment crystallizable (Fc) region is shown to vary in different physiological and pathological states.
32195245	2	34	theme	physiological	298:310	arg1	states					329:334	different physiological and pathological states	288:334	different physiological and pathological states	288:334	Glycosylation of the IgG fragment crystallizable (Fc) region is shown to vary in different physiological and pathological states.
32195245	5	35	theme	mice	777:780	arg1	glycoprofiles					721:733	the subclass-specific Fc IgG glycoprofiles	692:733	the subclass-specific Fc IgG glycoprofiles of healthy male and female FcγR knock-out mice on C57BL/6 and BALB/c backgrounds	692:814	To explore this, we examined the subclass-specific Fc IgG glycoprofiles of healthy male and female FcγR knock-out mice on C57BL/6 and BALB/c backgrounds.
32195245	0	36	theme	FcγR	33:36	arg1	Mice					48:51	FcγR Knock-Out Mice	33:51	FcγR Knock-Out Mice	33:51	Fc-Linked IgG N-Glycosylation in FcγR Knock-Out Mice.
32195245	6	37	theme	different	875:883	arg1	knock-outs					885:894	different knock-outs	875:894	different knock-outs	875:894	We observed slight changes in IgG Fc N-glycan profiles in different knock-outs; however, it seems that the strain background and sex have a stronger effect on N-glycosylation of IgG Fc regions than the FcγR repertoire.
32195245	5	38	theme	IgG	717:719	arg1	glycoprofiles					721:733	the subclass-specific Fc IgG glycoprofiles	692:733	the subclass-specific Fc IgG glycoprofiles of healthy male and female FcγR knock-out mice on C57BL/6 and BALB/c backgrounds	692:814	To explore this, we examined the subclass-specific Fc IgG glycoprofiles of healthy male and female FcγR knock-out mice on C57BL/6 and BALB/c backgrounds.
32195245	1	39	dep	pathogenesis	157:168	arg1	the					153:155	the	153:155	the	153:155	Immunoglobulin G (IgG) is the most abundant immunoglobulin isotype in the blood and is involved in the pathogenesis and progression of various diseases.
32195245	1	40	theme	various	189:195	arg1	diseases					197:204	various diseases	189:204	various diseases	189:204	Immunoglobulin G (IgG) is the most abundant immunoglobulin isotype in the blood and is involved in the pathogenesis and progression of various diseases.
32195245	6	41	from	profiles	863:870	arg1	knock-outs					885:894	different knock-outs	875:894	different knock-outs	875:894	We observed slight changes in IgG Fc N-glycan profiles in different knock-outs; however, it seems that the strain background and sex have a stronger effect on N-glycosylation of IgG Fc regions than the FcγR repertoire.
32195245	4	42	link	Fc-linked	584:592	arg1	N-glycome					594:602	the Fc-linked N-glycome	580:602	the Fc-linked N-glycome	580:602	However, it is not known whether IgG glycosylation is affected by the available repertoire of FcγRs, and if the Fc-linked N-glycome can compensate for modulation of the IgG-FcγR interaction.
32195245	3	43	theme	Fcγ	449:451	arg1	FcγRs					464:468	FcγRs	464:468	FcγRs	464:468	Fc N-glycan composition can alter the effector functions of IgG by modulating its affinity for ligands, such as Fcγ receptors (FcγRs).
32195245	3	43	theme	Fcγ	449:451	arg1	receptors					453:461	Fcγ receptors	449:461	Fcγ receptors (FcγRs)	449:469	Fc N-glycan composition can alter the effector functions of IgG by modulating its affinity for ligands, such as Fcγ receptors (FcγRs).
32195245	3	44	theme	effector	375:382	arg1	functions					384:392	the effector functions	371:392	the effector functions of IgG	371:399	Fc N-glycan composition can alter the effector functions of IgG by modulating its affinity for ligands, such as Fcγ receptors (FcγRs).
32195245	6	45	theme	Fc	851:852	arg1	profiles					863:870	IgG Fc N-glycan profiles	847:870	IgG Fc N-glycan profiles in different knock-outs	847:894	We observed slight changes in IgG Fc N-glycan profiles in different knock-outs; however, it seems that the strain background and sex have a stronger effect on N-glycosylation of IgG Fc regions than the FcγR repertoire.
32195245	1	46	theme	immunoglobulin	98:111	arg1	isotype					113:119	the most abundant immunoglobulin isotype	80:119	the most abundant immunoglobulin isotype in the blood	80:132	Immunoglobulin G (IgG) is the most abundant immunoglobulin isotype in the blood and is involved in the pathogenesis and progression of various diseases.
32195245	6	47	theme	N-glycan	854:861	arg1	profiles					863:870	IgG Fc N-glycan profiles	847:870	IgG Fc N-glycan profiles in different knock-outs	847:894	We observed slight changes in IgG Fc N-glycan profiles in different knock-outs; however, it seems that the strain background and sex have a stronger effect on N-glycosylation of IgG Fc regions than the FcγR repertoire.
32195245	4	48	theme	IgG-FcγR	641:648	arg1	interaction					650:660	the IgG-FcγR interaction	637:660	the IgG-FcγR interaction	637:660	However, it is not known whether IgG glycosylation is affected by the available repertoire of FcγRs, and if the Fc-linked N-glycome can compensate for modulation of the IgG-FcγR interaction.
32195245	2	49	theme	crystallizable	241:254	arg1	region					261:266	the IgG fragment crystallizable (Fc) region	224:266	the IgG fragment crystallizable (Fc) region	224:266	Glycosylation of the IgG fragment crystallizable (Fc) region is shown to vary in different physiological and pathological states.
32195245	2	50	theme	different	288:296	arg1	states					329:334	different physiological and pathological states	288:334	different physiological and pathological states	288:334	Glycosylation of the IgG fragment crystallizable (Fc) region is shown to vary in different physiological and pathological states.
32195245	6	51	theme	FcγR	1019:1022	arg1	repertoire					1024:1033	the FcγR repertoire	1015:1033	the FcγR repertoire	1015:1033	We observed slight changes in IgG Fc N-glycan profiles in different knock-outs; however, it seems that the strain background and sex have a stronger effect on N-glycosylation of IgG Fc regions than the FcγR repertoire.
32195245	1	52	theme	diseases	197:204	arg1	progression					174:184	progression	174:184	progression	174:184	Immunoglobulin G (IgG) is the most abundant immunoglobulin isotype in the blood and is involved in the pathogenesis and progression of various diseases.
32195245	1	52	theme	diseases	197:204	arg1	pathogenesis					157:168	pathogenesis	157:168	pathogenesis	157:168	Immunoglobulin G (IgG) is the most abundant immunoglobulin isotype in the blood and is involved in the pathogenesis and progression of various diseases.
32195245	2	53	theme	region	261:266	arg1	Glycosylation					207:219	Glycosylation	207:219	Glycosylation of the IgG fragment crystallizable (Fc) region	207:266	Glycosylation of the IgG fragment crystallizable (Fc) region is shown to vary in different physiological and pathological states.
32195245	4	54	theme	interaction	650:660	arg1	modulation					623:632	modulation	623:632	modulation of the IgG-FcγR interaction	623:660	However, it is not known whether IgG glycosylation is affected by the available repertoire of FcγRs, and if the Fc-linked N-glycome can compensate for modulation of the IgG-FcγR interaction.
32195245	2	55	theme	IgG	228:230	arg1	region					261:266	the IgG fragment crystallizable (Fc) region	224:266	the IgG fragment crystallizable (Fc) region	224:266	Glycosylation of the IgG fragment crystallizable (Fc) region is shown to vary in different physiological and pathological states.
32195245	6	56	from	changes	836:842	arg1	profiles					863:870	IgG Fc N-glycan profiles	847:870	IgG Fc N-glycan profiles in different knock-outs	847:894	We observed slight changes in IgG Fc N-glycan profiles in different knock-outs; however, it seems that the strain background and sex have a stronger effect on N-glycosylation of IgG Fc regions than the FcγR repertoire.
32195245	5	57	from	glycoprofiles	721:733	arg1	backgrounds					804:814	C57BL/6 and BALB/c backgrounds	785:814	C57BL/6 and BALB/c backgrounds	785:814	To explore this, we examined the subclass-specific Fc IgG glycoprofiles of healthy male and female FcγR knock-out mice on C57BL/6 and BALB/c backgrounds.
32195245	4	58	theme	available	542:550	arg1	FcγRs					566:570	FcγRs	566:570	FcγRs	566:570	However, it is not known whether IgG glycosylation is affected by the available repertoire of FcγRs, and if the Fc-linked N-glycome can compensate for modulation of the IgG-FcγR interaction.
32195245	4	58	theme	available	542:550	arg1	repertoire					552:561	the available repertoire	538:561	the available repertoire of FcγRs	538:570	However, it is not known whether IgG glycosylation is affected by the available repertoire of FcγRs, and if the Fc-linked N-glycome can compensate for modulation of the IgG-FcγR interaction.
32195245	6	59	theme	strain	924:929	arg1	background					931:940	the strain background	920:940	the strain background	920:940	We observed slight changes in IgG Fc N-glycan profiles in different knock-outs; however, it seems that the strain background and sex have a stronger effect on N-glycosylation of IgG Fc regions than the FcγR repertoire.
33354574	8	0	theme	gut	1053:1055	arg1	microbiota					1057:1066	the gut microbiota	1049:1066	the gut microbiota	1049:1066	Furthermore, EFH recovered the diversity and balance of the gut microbiota.
33354574	9	1	theme	NF-κB	1201:1205	arg1	pathway					1207:1213	the NF-κB pathway	1197:1213	the NF-κB pathway	1197:1213	CONCLUSIONS EFH has protective effects against DSS-induced colitis by keeping the balance of the gut microbiota and suppressing the NF-κB pathway.
33354574	4	2	theme	gut	606:608	arg1	microbiota					610:619	The gut microbiota	602:619	The gut microbiota	602:619	The gut microbiota was characterized by 16S rRNA gene sequencing and analysis.
33354574	5	3	from	rich	728:731	arg1	flavones					750:757	flavones	750:757	flavones	750:757	RESULTS LC-ESI-MS analysis showed that EFH was rich in alkaloids and flavones.
33354574	5	3	from	rich	728:731	arg1	alkaloids					736:744	alkaloids	736:744	alkaloids	736:744	RESULTS LC-ESI-MS analysis showed that EFH was rich in alkaloids and flavones.
33354574	5	4	theme	RESULTS	681:687	arg1	analysis					699:706	RESULTS LC-ESI-MS analysis	681:706	RESULTS LC-ESI-MS analysis	681:706	RESULTS LC-ESI-MS analysis showed that EFH was rich in alkaloids and flavones.
33354574	0	5	from	Effects	18:24	arg1	Sulfate					68:74	Dextran Sulfate Sodium- (DSS-)	60:89	Dextran Sulfate Sodium- (DSS-)	60:89	Anti-Inflammatory Effects of Heritiera littoralis Fruits on Dextran Sulfate Sodium- (DSS-) Induced Ulcerative Colitis in Mice by Regulating Gut Microbiota and Suppressing NF-κB Pathway.
33354574	0	6	theme	NF-κB	171:175	arg1	Pathway					177:183	NF-κB Pathway	171:183	NF-κB Pathway	171:183	Anti-Inflammatory Effects of Heritiera littoralis Fruits on Dextran Sulfate Sodium- (DSS-) Induced Ulcerative Colitis in Mice by Regulating Gut Microbiota and Suppressing NF-κB Pathway.
33354574	6	7	theme	DAI	818:820	arg1	score					822:826	the DAI score	814:826	the DAI score	814:826	The results indicated that EFH significantly improved the DAI score, relieved colon shortening, and repaired pathological colonic variations in colitis.
33354574	9	8	theme	protective	1089:1098	arg1	effects					1100:1106	protective effects	1089:1106	protective effects against DSS-induced colitis	1089:1134	CONCLUSIONS EFH has protective effects against DSS-induced colitis by keeping the balance of the gut microbiota and suppressing the NF-κB pathway.
33354574	2	9	theme	3	288:288	arg1	%					289:289	%	289:289	%	289:289	The mice with 3% DSS-induced UC were administered EFH (200, 400, and 800 mg/kg), sulfasalazine (SASP, 200 mg/kg), and azathioprine (AZA, 13 mg/kg) for 10 days via daily gavage.
33354574	0	10	dep	Sulfate	68:74	arg1	DSS-					85:88	DSS-	85:88	DSS-	85:88	Anti-Inflammatory Effects of Heritiera littoralis Fruits on Dextran Sulfate Sodium- (DSS-) Induced Ulcerative Colitis in Mice by Regulating Gut Microbiota and Suppressing NF-κB Pathway.
33354574	0	10	dep	Sulfate	68:74	arg1	Sodium-					76:82	Sodium-	76:82	Dextran Sulfate Sodium- (DSS-)	60:89	Anti-Inflammatory Effects of Heritiera littoralis Fruits on Dextran Sulfate Sodium- (DSS-) Induced Ulcerative Colitis in Mice by Regulating Gut Microbiota and Suppressing NF-κB Pathway.
33354574	4	11	theme	rRNA	646:649	arg1	sequencing					656:665	rRNA gene sequencing	646:665	rRNA gene sequencing	646:665	The gut microbiota was characterized by 16S rRNA gene sequencing and analysis.
33354574	3	12	theme	mediators	591:599	arg1	scores					556:561	histological scores	543:561	histological scores	543:561	The colonic inflammation was evaluated by the disease activity index (DAI), colonic length, histological scores, and levels of inflammatory mediators.
33354574	3	12	theme	mediators	591:599	arg1	levels					568:573	levels	568:573	levels of inflammatory mediators	568:599	The colonic inflammation was evaluated by the disease activity index (DAI), colonic length, histological scores, and levels of inflammatory mediators.
33354574	3	12	theme	mediators	591:599	arg1	DAI					521:523	DAI	521:523	DAI	521:523	The colonic inflammation was evaluated by the disease activity index (DAI), colonic length, histological scores, and levels of inflammatory mediators.
33354574	3	12	theme	mediators	591:599	arg1	index					514:518	the disease activity index	493:518	the disease activity index (DAI)	493:524	The colonic inflammation was evaluated by the disease activity index (DAI), colonic length, histological scores, and levels of inflammatory mediators.
33354574	3	12	theme	mediators	591:599	arg1	length					535:540	colonic length	527:540	colonic length	527:540	The colonic inflammation was evaluated by the disease activity index (DAI), colonic length, histological scores, and levels of inflammatory mediators.
33354574	2	13	theme	daily	437:441	arg1	gavage					443:448	daily gavage	437:448	daily gavage	437:448	The mice with 3% DSS-induced UC were administered EFH (200, 400, and 800 mg/kg), sulfasalazine (SASP, 200 mg/kg), and azathioprine (AZA, 13 mg/kg) for 10 days via daily gavage.
33354574	0	14	theme	Ulcerative	99:108	arg1	Colitis					110:116	Ulcerative Colitis	99:116	Ulcerative Colitis in Mice	99:124	Anti-Inflammatory Effects of Heritiera littoralis Fruits on Dextran Sulfate Sodium- (DSS-) Induced Ulcerative Colitis in Mice by Regulating Gut Microbiota and Suppressing NF-κB Pathway.
33354574	5	15	theme	LC-ESI-MS	689:697	arg1	analysis					699:706	RESULTS LC-ESI-MS analysis	681:706	RESULTS LC-ESI-MS analysis	681:706	RESULTS LC-ESI-MS analysis showed that EFH was rich in alkaloids and flavones.
33354574	9	16	theme	DSS-induced	1116:1126	arg1	colitis					1128:1134	DSS-induced colitis	1116:1134	DSS-induced colitis	1116:1134	CONCLUSIONS EFH has protective effects against DSS-induced colitis by keeping the balance of the gut microbiota and suppressing the NF-κB pathway.
33354574	3	17	theme	colonic	455:461	arg1	inflammation					463:474	The colonic inflammation	451:474	The colonic inflammation	451:474	The colonic inflammation was evaluated by the disease activity index (DAI), colonic length, histological scores, and levels of inflammatory mediators.
33354574	8	18	dep	diversity	1024:1032	arg1	the					1020:1022	the	1020:1022	the	1020:1022	Furthermore, EFH recovered the diversity and balance of the gut microbiota.
33354574	2	19	dep	azathioprine	392:403	arg1	AZA					406:408	AZA	406:408	AZA	406:408	The mice with 3% DSS-induced UC were administered EFH (200, 400, and 800 mg/kg), sulfasalazine (SASP, 200 mg/kg), and azathioprine (AZA, 13 mg/kg) for 10 days via daily gavage.
33354574	2	19	dep	azathioprine	392:403	arg1	13 mg/kg					411:418	13 mg/kg	411:418	13 mg/kg	411:418	The mice with 3% DSS-induced UC were administered EFH (200, 400, and 800 mg/kg), sulfasalazine (SASP, 200 mg/kg), and azathioprine (AZA, 13 mg/kg) for 10 days via daily gavage.
33354574	2	20	dep	EFH	324:326	arg1	400					334:336	400	334:336	400	334:336	The mice with 3% DSS-induced UC were administered EFH (200, 400, and 800 mg/kg), sulfasalazine (SASP, 200 mg/kg), and azathioprine (AZA, 13 mg/kg) for 10 days via daily gavage.
33354574	2	20	dep	EFH	324:326	arg1	800 mg/kg					343:351	800 mg/kg	343:351	800 mg/kg	343:351	The mice with 3% DSS-induced UC were administered EFH (200, 400, and 800 mg/kg), sulfasalazine (SASP, 200 mg/kg), and azathioprine (AZA, 13 mg/kg) for 10 days via daily gavage.
33354574	2	20	dep	EFH	324:326	arg1	200					329:331	200	329:331	200	329:331	The mice with 3% DSS-induced UC were administered EFH (200, 400, and 800 mg/kg), sulfasalazine (SASP, 200 mg/kg), and azathioprine (AZA, 13 mg/kg) for 10 days via daily gavage.
33354574	0	21	theme	Anti-Inflammatory	0:16	arg1	Effects					18:24	Anti-Inflammatory Effects	0:24	Anti-Inflammatory Effects of Heritiera littoralis Fruits on Dextran Sulfate Sodium- (DSS-)	0:89	Anti-Inflammatory Effects of Heritiera littoralis Fruits on Dextran Sulfate Sodium- (DSS-) Induced Ulcerative Colitis in Mice by Regulating Gut Microbiota and Suppressing NF-κB Pathway.
33354574	3	22	theme	disease	497:503	arg1	DAI					521:523	DAI	521:523	DAI	521:523	The colonic inflammation was evaluated by the disease activity index (DAI), colonic length, histological scores, and levels of inflammatory mediators.
33354574	3	22	theme	disease	497:503	arg1	index					514:518	the disease activity index	493:518	the disease activity index (DAI)	493:524	The colonic inflammation was evaluated by the disease activity index (DAI), colonic length, histological scores, and levels of inflammatory mediators.
33354574	0	23	theme	Heritiera	29:37	arg1	Fruits					50:55	Heritiera littoralis Fruits	29:55	Heritiera littoralis Fruits	29:55	Anti-Inflammatory Effects of Heritiera littoralis Fruits on Dextran Sulfate Sodium- (DSS-) Induced Ulcerative Colitis in Mice by Regulating Gut Microbiota and Suppressing NF-κB Pathway.
33354574	6	24	theme	colonic	882:888	arg1	variations					890:899	pathological colonic variations	869:899	pathological colonic variations in colitis	869:910	The results indicated that EFH significantly improved the DAI score, relieved colon shortening, and repaired pathological colonic variations in colitis.
33354574	2	25	theme	DSS-induced	291:301	arg1	UC					303:304	3% DSS-induced UC	288:304	3% DSS-induced UC	288:304	The mice with 3% DSS-induced UC were administered EFH (200, 400, and 800 mg/kg), sulfasalazine (SASP, 200 mg/kg), and azathioprine (AZA, 13 mg/kg) for 10 days via daily gavage.
33354574	6	26	theme	pathological	869:880	arg1	variations					890:899	pathological colonic variations	869:899	pathological colonic variations in colitis	869:910	The results indicated that EFH significantly improved the DAI score, relieved colon shortening, and repaired pathological colonic variations in colitis.
33354574	2	27	theme	%	289:289	arg1	UC					303:304	3% DSS-induced UC	288:304	3% DSS-induced UC	288:304	The mice with 3% DSS-induced UC were administered EFH (200, 400, and 800 mg/kg), sulfasalazine (SASP, 200 mg/kg), and azathioprine (AZA, 13 mg/kg) for 10 days via daily gavage.
33354574	0	28	theme	Fruits	50:55	arg1	Effects					18:24	Anti-Inflammatory Effects	0:24	Anti-Inflammatory Effects of Heritiera littoralis Fruits on Dextran Sulfate Sodium- (DSS-)	0:89	Anti-Inflammatory Effects of Heritiera littoralis Fruits on Dextran Sulfate Sodium- (DSS-) Induced Ulcerative Colitis in Mice by Regulating Gut Microbiota and Suppressing NF-κB Pathway.
33354574	6	29	from	variations	890:899	arg1	colitis					904:910	colitis	904:910	colitis	904:910	The results indicated that EFH significantly improved the DAI score, relieved colon shortening, and repaired pathological colonic variations in colitis.
33354574	7	30	from	proteins	926:933	arg1	pathway					948:954	the NF-κB pathway	938:954	the NF-κB pathway	938:954	In addition, proteins in the NF-κB pathway were significantly inhibited by EFH.
33354574	0	31	theme	littoralis	39:48	arg1	Fruits					50:55	Heritiera littoralis Fruits	29:55	Heritiera littoralis Fruits	29:55	Anti-Inflammatory Effects of Heritiera littoralis Fruits on Dextran Sulfate Sodium- (DSS-) Induced Ulcerative Colitis in Mice by Regulating Gut Microbiota and Suppressing NF-κB Pathway.
33354574	1	32	dep	MATERIALS	186:194	arg1	compositions					221:232	The chemical compositions	208:232	The chemical compositions	208:232	MATERIALS AND METHODS The chemical compositions of EFH were identified using LC-ESI-MS.
33354574	5	33	from	flavones	750:757	arg1	rich					728:731	rich	728:731	rich	728:731	RESULTS LC-ESI-MS analysis showed that EFH was rich in alkaloids and flavones.
33354574	1	34	theme	chemical	212:219	arg1	compositions					221:232	The chemical compositions	208:232	The chemical compositions	208:232	MATERIALS AND METHODS The chemical compositions of EFH were identified using LC-ESI-MS.
33354574	0	35	theme	Dextran	60:66	arg1	Sulfate					68:74	Dextran Sulfate Sodium- (DSS-)	60:89	Dextran Sulfate Sodium- (DSS-)	60:89	Anti-Inflammatory Effects of Heritiera littoralis Fruits on Dextran Sulfate Sodium- (DSS-) Induced Ulcerative Colitis in Mice by Regulating Gut Microbiota and Suppressing NF-κB Pathway.
33354574	3	36	theme	histological	543:554	arg1	scores					556:561	histological scores	543:561	histological scores	543:561	The colonic inflammation was evaluated by the disease activity index (DAI), colonic length, histological scores, and levels of inflammatory mediators.
33354574	3	37	theme	activity	505:512	arg1	DAI					521:523	DAI	521:523	DAI	521:523	The colonic inflammation was evaluated by the disease activity index (DAI), colonic length, histological scores, and levels of inflammatory mediators.
33354574	3	37	theme	activity	505:512	arg1	index					514:518	the disease activity index	493:518	the disease activity index (DAI)	493:524	The colonic inflammation was evaluated by the disease activity index (DAI), colonic length, histological scores, and levels of inflammatory mediators.
33354574	9	38	theme	gut	1166:1168	arg1	microbiota					1170:1179	the gut microbiota	1162:1179	the gut microbiota	1162:1179	CONCLUSIONS EFH has protective effects against DSS-induced colitis by keeping the balance of the gut microbiota and suppressing the NF-κB pathway.
33354574	0	39	theme	Gut	140:142	arg1	Microbiota					144:153	Gut Microbiota	140:153	Gut Microbiota	140:153	Anti-Inflammatory Effects of Heritiera littoralis Fruits on Dextran Sulfate Sodium- (DSS-) Induced Ulcerative Colitis in Mice by Regulating Gut Microbiota and Suppressing NF-κB Pathway.
33354574	0	40	from	Colitis	110:116	arg1	Mice					121:124	Mice	121:124	Mice	121:124	Anti-Inflammatory Effects of Heritiera littoralis Fruits on Dextran Sulfate Sodium- (DSS-) Induced Ulcerative Colitis in Mice by Regulating Gut Microbiota and Suppressing NF-κB Pathway.
33354574	9	41	theme	microbiota	1170:1179	arg1	balance					1151:1157	the balance	1147:1157	the balance of the gut microbiota	1147:1179	CONCLUSIONS EFH has protective effects against DSS-induced colitis by keeping the balance of the gut microbiota and suppressing the NF-κB pathway.
33354574	5	42	from	alkaloids	736:744	arg1	rich					728:731	rich	728:731	rich	728:731	RESULTS LC-ESI-MS analysis showed that EFH was rich in alkaloids and flavones.
33354574	6	43	theme	colon	838:842	arg1	shortening					844:853	colon shortening	838:853	colon shortening	838:853	The results indicated that EFH significantly improved the DAI score, relieved colon shortening, and repaired pathological colonic variations in colitis.
33354574	3	44	theme	inflammatory	578:589	arg1	mediators					591:599	inflammatory mediators	578:599	inflammatory mediators	578:599	The colonic inflammation was evaluated by the disease activity index (DAI), colonic length, histological scores, and levels of inflammatory mediators.
33354574	3	45	theme	colonic	527:533	arg1	length					535:540	colonic length	527:540	colonic length	527:540	The colonic inflammation was evaluated by the disease activity index (DAI), colonic length, histological scores, and levels of inflammatory mediators.
33354574	2	46	with	mice	278:281	arg1	UC					303:304	3% DSS-induced UC	288:304	3% DSS-induced UC	288:304	The mice with 3% DSS-induced UC were administered EFH (200, 400, and 800 mg/kg), sulfasalazine (SASP, 200 mg/kg), and azathioprine (AZA, 13 mg/kg) for 10 days via daily gavage.
33354574	7	47	theme	NF-κB	942:946	arg1	pathway					948:954	the NF-κB pathway	938:954	the NF-κB pathway	938:954	In addition, proteins in the NF-κB pathway were significantly inhibited by EFH.
33354574	4	48	theme	gene	651:654	arg1	sequencing					656:665	rRNA gene sequencing	646:665	rRNA gene sequencing	646:665	The gut microbiota was characterized by 16S rRNA gene sequencing and analysis.
33354574	2	49	dep	sulfasalazine	355:367	arg1	SASP					370:373	SASP	370:373	SASP	370:373	The mice with 3% DSS-induced UC were administered EFH (200, 400, and 800 mg/kg), sulfasalazine (SASP, 200 mg/kg), and azathioprine (AZA, 13 mg/kg) for 10 days via daily gavage.
33354574	2	49	dep	sulfasalazine	355:367	arg1	200 mg/kg					376:384	200 mg/kg	376:384	200 mg/kg	376:384	The mice with 3% DSS-induced UC were administered EFH (200, 400, and 800 mg/kg), sulfasalazine (SASP, 200 mg/kg), and azathioprine (AZA, 13 mg/kg) for 10 days via daily gavage.
33354574	1	50	theme	EFH	237:239	arg1	METHODS					200:206	METHODS	200:206	METHODS	200:206	MATERIALS AND METHODS The chemical compositions of EFH were identified using LC-ESI-MS.
33354574	1	50	theme	EFH	237:239	arg1	MATERIALS					186:194	MATERIALS	186:194	MATERIALS	186:194	MATERIALS AND METHODS The chemical compositions of EFH were identified using LC-ESI-MS.
33354574	9	51	contain	has	1085:1087	arg2	effects					1100:1106	protective effects	1089:1106	protective effects against DSS-induced colitis	1089:1134	CONCLUSIONS EFH has protective effects against DSS-induced colitis by keeping the balance of the gut microbiota and suppressing the NF-κB pathway.
33354574	9	51	contain	has	1085:1087	arg1	EFH					1081:1083	CONCLUSIONS EFH	1069:1083	CONCLUSIONS EFH	1069:1083	CONCLUSIONS EFH has protective effects against DSS-induced colitis by keeping the balance of the gut microbiota and suppressing the NF-κB pathway.
33354574	9	52	theme	CONCLUSIONS	1069:1079	arg1	EFH					1081:1083	CONCLUSIONS EFH	1069:1083	CONCLUSIONS EFH	1069:1083	CONCLUSIONS EFH has protective effects against DSS-induced colitis by keeping the balance of the gut microbiota and suppressing the NF-κB pathway.
33354574	8	53	theme	microbiota	1057:1066	arg1	balance					1038:1044	balance	1038:1044	balance	1038:1044	Furthermore, EFH recovered the diversity and balance of the gut microbiota.
33354574	8	53	theme	microbiota	1057:1066	arg1	diversity					1024:1032	diversity	1024:1032	diversity	1024:1032	Furthermore, EFH recovered the diversity and balance of the gut microbiota.
32310329	0	0	theme	Coagulation	97:107	arg1	V					116:116	human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V	48:116	human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV)	48:121	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	0	0	theme	Coagulation	97:107	arg1	FV					119:120	FV	119:120	FV	119:120	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	1	1	theme	poor	266:269	arg1	clotting					271:278	poor clotting	266:278	poor clotting after an injury or surgery	266:305	FV deficiency is a rare bleeding disorder that results in poor clotting after an injury or surgery.
32310329	7	2	theme	future	1126:1131	arg1	studies					1144:1150	future functional studies	1126:1150	future functional studies	1126:1150	Such glycosylation details are fundamental for future functional studies and therapeutics development.
32310329	8	3	with	proteins	1283:1290	arg1	acids					1318:1322	more than 2000 amino acids	1297:1322	more than 2000 amino acids	1297:1322	In addition, the established methodology can be readily applied to analyze glycosylation patterns of proteins with more than 2000 amino acids.
32310329	5	4	theme	alternated	860:869	arg1	analysis					903:910	alternated fragmentation mass spectrometry analysis	860:910	alternated fragmentation mass spectrometry analysis	860:910	METHODS In this study, we comprehensively mapped the glycosylation patterns of human plasma-derived FV by combining multienzyme digestion, hydrophilic interaction chromatography enrichment of glycopeptides, and alternated fragmentation mass spectrometry analysis.
32310329	1	5	theme	rare	227:230	arg1	disorder					241:248	a rare bleeding disorder	225:248	a rare bleeding disorder that results in poor clotting after an injury or surgery	225:305	FV deficiency is a rare bleeding disorder that results in poor clotting after an injury or surgery.
32310329	1	5	theme	rare	227:230	arg1	deficiency					211:220	FV deficiency	208:220	FV deficiency	208:220	FV deficiency is a rare bleeding disorder that results in poor clotting after an injury or surgery.
32310329	0	6	theme	BACKGROUND/OBJECTIVE	76:95	arg1	V					116:116	human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V	48:116	human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV)	48:121	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	0	6	theme	BACKGROUND/OBJECTIVE	76:95	arg1	FV					119:120	FV	119:120	FV	119:120	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	0	7	theme	essential	158:166	arg1	O-glycosylation					21:35	O-glycosylation	21:35	O-glycosylation	21:35	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	0	7	theme	essential	158:166	arg1	cofactor					168:175	an essential cofactor	155:175	an essential cofactor in the blood clotting cascade	155:205	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	0	7	theme	essential	158:166	arg1	N-					14:15	Comprehensive N-	0:15	Comprehensive N-	0:15	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	3	8	theme	in	485:486	arg1	rate					503:506	in vivo clearance rate	485:506	in vivo clearance rate	485:506	Glycosylation affects the biological activity, pharmacokinetics, immunogenicity, and in vivo clearance rate of proteins in the plasma.
32310329	1	9	theme	bleeding	232:239	arg1	disorder					241:248	a rare bleeding disorder	225:248	a rare bleeding disorder that results in poor clotting after an injury or surgery	225:305	FV deficiency is a rare bleeding disorder that results in poor clotting after an injury or surgery.
32310329	1	9	theme	bleeding	232:239	arg1	deficiency					211:220	FV deficiency	208:220	FV deficiency	208:220	FV deficiency is a rare bleeding disorder that results in poor clotting after an injury or surgery.
32310329	0	10	theme	V	116:116	arg1	O-glycosylation					21:35	O-glycosylation	21:35	O-glycosylation	21:35	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	0	10	theme	V	116:116	arg1	N-					14:15	Comprehensive N-	0:15	Comprehensive N-	0:15	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	0	10	theme	V	116:116	arg1	glycoprotein					138:149	a multidomain glycoprotein	124:149	a multidomain glycoprotein	124:149	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	0	10	theme	V	116:116	arg1	cofactor					168:175	an essential cofactor	155:175	an essential cofactor in the blood clotting cascade	155:205	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	5	11	gly	glycopeptides	841:853	arg2	glycopeptides					841:853	glycopeptides	841:853	glycopeptides	841:853	METHODS In this study, we comprehensively mapped the glycosylation patterns of human plasma-derived FV by combining multienzyme digestion, hydrophilic interaction chromatography enrichment of glycopeptides, and alternated fragmentation mass spectrometry analysis.
32310329	3	12	theme	proteins	511:518	arg1	immunogenicity					465:478	immunogenicity	465:478	immunogenicity	465:478	Glycosylation affects the biological activity, pharmacokinetics, immunogenicity, and in vivo clearance rate of proteins in the plasma.
32310329	3	12	theme	proteins	511:518	arg1	rate					503:506	in vivo clearance rate	485:506	in vivo clearance rate	485:506	Glycosylation affects the biological activity, pharmacokinetics, immunogenicity, and in vivo clearance rate of proteins in the plasma.
32310329	3	12	theme	proteins	511:518	arg1	activity					437:444	the biological activity	422:444	the biological activity	422:444	Glycosylation affects the biological activity, pharmacokinetics, immunogenicity, and in vivo clearance rate of proteins in the plasma.
32310329	3	12	theme	proteins	511:518	arg1	pharmacokinetics					447:462	pharmacokinetics	447:462	pharmacokinetics	447:462	Glycosylation affects the biological activity, pharmacokinetics, immunogenicity, and in vivo clearance rate of proteins in the plasma.
32310329	4	13	theme	FV	557:558	arg1	profile					546:552	The glycan profile	535:552	The glycan profile of FV, as well as how it affects the activity, stability, and immunogenicity,	535:630	The glycan profile of FV, as well as how it affects the activity, stability, and immunogenicity, remains unknown.
32310329	0	14	theme	factor	109:114	arg1	V					116:116	human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V	48:116	human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV)	48:121	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	0	14	theme	factor	109:114	arg1	FV					119:120	FV	119:120	FV	119:120	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	8	15	theme	established	1199:1209	arg1	methodology					1211:1221	the established methodology	1195:1221	the established methodology	1195:1221	In addition, the established methodology can be readily applied to analyze glycosylation patterns of proteins with more than 2000 amino acids.
32310329	5	16	theme	mass	885:888	arg1	analysis					903:910	alternated fragmentation mass spectrometry analysis	860:910	alternated fragmentation mass spectrometry analysis	860:910	METHODS In this study, we comprehensively mapped the glycosylation patterns of human plasma-derived FV by combining multienzyme digestion, hydrophilic interaction chromatography enrichment of glycopeptides, and alternated fragmentation mass spectrometry analysis.
32310329	0	17	theme	clotting	190:197	arg1	cascade					199:205	the blood clotting cascade	180:205	the blood clotting cascade	180:205	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	5	18	gly	glycosylation	702:714	arg1	FV					749:750	human plasma-derived FV	728:750	human plasma-derived FV	728:750	METHODS In this study, we comprehensively mapped the glycosylation patterns of human plasma-derived FV by combining multienzyme digestion, hydrophilic interaction chromatography enrichment of glycopeptides, and alternated fragmentation mass spectrometry analysis.
32310329	2	19	theme	fresh	359:363	arg1	plasma					372:377	fresh frozen plasma	359:377	fresh frozen plasma	359:377	The only treatment for the disease is infusions of fresh frozen plasma and blood platelets.
32310329	5	20	theme	plasma-derived	734:747	arg1	FV					749:750	human plasma-derived FV	728:750	human plasma-derived FV	728:750	METHODS In this study, we comprehensively mapped the glycosylation patterns of human plasma-derived FV by combining multienzyme digestion, hydrophilic interaction chromatography enrichment of glycopeptides, and alternated fragmentation mass spectrometry analysis.
32310329	0	21	theme	blood	184:188	arg1	cascade					199:205	the blood clotting cascade	180:205	the blood clotting cascade	180:205	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	3	22	from	pharmacokinetics	447:462	arg1	plasma					527:532	the plasma	523:532	the plasma	523:532	Glycosylation affects the biological activity, pharmacokinetics, immunogenicity, and in vivo clearance rate of proteins in the plasma.
32310329	5	23	theme	enrichment	827:836	arg1	chromatography					812:825	hydrophilic interaction chromatography enrichment	788:836	hydrophilic interaction chromatography enrichment of glycopeptides	788:853	METHODS In this study, we comprehensively mapped the glycosylation patterns of human plasma-derived FV by combining multienzyme digestion, hydrophilic interaction chromatography enrichment of glycopeptides, and alternated fragmentation mass spectrometry analysis.
32310329	5	24	theme	FV	749:750	arg1	patterns					716:723	the glycosylation patterns	698:723	the glycosylation patterns of human plasma-derived FV	698:750	METHODS In this study, we comprehensively mapped the glycosylation patterns of human plasma-derived FV by combining multienzyme digestion, hydrophilic interaction chromatography enrichment of glycopeptides, and alternated fragmentation mass spectrometry analysis.
32310329	5	25	theme	glycosylation	702:714	arg1	patterns					716:723	the glycosylation patterns	698:723	the glycosylation patterns of human plasma-derived FV	698:750	METHODS In this study, we comprehensively mapped the glycosylation patterns of human plasma-derived FV by combining multienzyme digestion, hydrophilic interaction chromatography enrichment of glycopeptides, and alternated fragmentation mass spectrometry analysis.
32310329	7	26	theme	Such	1079:1082	arg1	details					1098:1104	Such glycosylation details	1079:1104	Such glycosylation details	1079:1104	Such glycosylation details are fundamental for future functional studies and therapeutics development.
32310329	6	27	theme	O-glycan	1056:1063	arg1	compositions					1065:1076	40 N-glycan and 17 O-glycan compositions	1037:1076	compositions	1065:1076	RESULTS/CONCLUSION A total of 57 unique N-glycopeptides and 51 O-glycopeptides were identified, which were categorized into 40 N-glycan and 17 O-glycan compositions.
32310329	0	28	theme	Comprehensive	0:12	arg1	O-glycosylation					21:35	O-glycosylation	21:35	O-glycosylation	21:35	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	0	28	theme	Comprehensive	0:12	arg1	N-					14:15	Comprehensive N-	0:15	Comprehensive N-	0:15	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	0	28	theme	Comprehensive	0:12	arg1	glycoprotein					138:149	a multidomain glycoprotein	124:149	a multidomain glycoprotein	124:149	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	0	28	theme	Comprehensive	0:12	arg1	cofactor					168:175	an essential cofactor	155:175	an essential cofactor in the blood clotting cascade	155:205	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	7	29	theme	glycosylation	1084:1096	arg1	details					1098:1104	Such glycosylation details	1079:1104	Such glycosylation details	1079:1104	Such glycosylation details are fundamental for future functional studies and therapeutics development.
32310329	3	30	from	immunogenicity	465:478	arg1	plasma					527:532	the plasma	523:532	the plasma	523:532	Glycosylation affects the biological activity, pharmacokinetics, immunogenicity, and in vivo clearance rate of proteins in the plasma.
32310329	3	31	dep	in	485:486	arg1	vivo					488:491	vivo	488:491	vivo	488:491	Glycosylation affects the biological activity, pharmacokinetics, immunogenicity, and in vivo clearance rate of proteins in the plasma.
32310329	5	32	theme	glycopeptides	841:853	arg1	digestion					777:785	multienzyme digestion	765:785	multienzyme digestion	765:785	METHODS In this study, we comprehensively mapped the glycosylation patterns of human plasma-derived FV by combining multienzyme digestion, hydrophilic interaction chromatography enrichment of glycopeptides, and alternated fragmentation mass spectrometry analysis.
32310329	5	32	theme	glycopeptides	841:853	arg1	analysis					903:910	alternated fragmentation mass spectrometry analysis	860:910	alternated fragmentation mass spectrometry analysis	860:910	METHODS In this study, we comprehensively mapped the glycosylation patterns of human plasma-derived FV by combining multienzyme digestion, hydrophilic interaction chromatography enrichment of glycopeptides, and alternated fragmentation mass spectrometry analysis.
32310329	5	32	theme	glycopeptides	841:853	arg1	chromatography					812:825	hydrophilic interaction chromatography enrichment	788:836	hydrophilic interaction chromatography enrichment of glycopeptides	788:853	METHODS In this study, we comprehensively mapped the glycosylation patterns of human plasma-derived FV by combining multienzyme digestion, hydrophilic interaction chromatography enrichment of glycopeptides, and alternated fragmentation mass spectrometry analysis.
32310329	6	33	theme	N-glycopeptides	953:967	arg1	total					934:938	A total	932:938	A total of 57 unique N-glycopeptides and 51 O-glycopeptides	932:990	RESULTS/CONCLUSION A total of 57 unique N-glycopeptides and 51 O-glycopeptides were identified, which were categorized into 40 N-glycan and 17 O-glycan compositions.
32310329	6	34	theme	unique	946:951	arg1	N-glycopeptides					953:967	57 unique N-glycopeptides	943:967	57 unique N-glycopeptides	943:967	RESULTS/CONCLUSION A total of 57 unique N-glycopeptides and 51 O-glycopeptides were identified, which were categorized into 40 N-glycan and 17 O-glycan compositions.
32310329	8	35	theme	amino	1312:1316	arg1	acids					1318:1322	more than 2000 amino acids	1297:1322	more than 2000 amino acids	1297:1322	In addition, the established methodology can be readily applied to analyze glycosylation patterns of proteins with more than 2000 amino acids.
32310329	5	36	theme	human	728:732	arg1	FV					749:750	human plasma-derived FV	728:750	human plasma-derived FV	728:750	METHODS In this study, we comprehensively mapped the glycosylation patterns of human plasma-derived FV by combining multienzyme digestion, hydrophilic interaction chromatography enrichment of glycopeptides, and alternated fragmentation mass spectrometry analysis.
32310329	2	37	theme	blood	383:387	arg1	platelets					389:397	blood platelets	383:397	blood platelets	383:397	The only treatment for the disease is infusions of fresh frozen plasma and blood platelets.
32310329	2	38	theme	platelets	389:397	arg1	infusions					346:354	infusions	346:354	infusions of fresh frozen plasma and blood platelets	346:397	The only treatment for the disease is infusions of fresh frozen plasma and blood platelets.
32310329	2	38	theme	platelets	389:397	arg1	treatment					317:325	The only treatment	308:325	The only treatment for the disease	308:341	The only treatment for the disease is infusions of fresh frozen plasma and blood platelets.
32310329	0	39	from	cofactor	168:175	arg1	cascade					199:205	the blood clotting cascade	180:205	the blood clotting cascade	180:205	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	5	40	theme	fragmentation	871:883	arg1	analysis					903:910	alternated fragmentation mass spectrometry analysis	860:910	alternated fragmentation mass spectrometry analysis	860:910	METHODS In this study, we comprehensively mapped the glycosylation patterns of human plasma-derived FV by combining multienzyme digestion, hydrophilic interaction chromatography enrichment of glycopeptides, and alternated fragmentation mass spectrometry analysis.
32310329	8	41	theme	proteins	1283:1290	arg1	patterns					1271:1278	glycosylation patterns	1257:1278	glycosylation patterns of proteins with more than 2000 amino acids	1257:1322	In addition, the established methodology can be readily applied to analyze glycosylation patterns of proteins with more than 2000 amino acids.
32310329	0	42	theme	multidomain	126:136	arg1	N-					14:15	Comprehensive N-	0:15	Comprehensive N-	0:15	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	0	42	theme	multidomain	126:136	arg1	glycoprotein					138:149	a multidomain glycoprotein	124:149	a multidomain glycoprotein	124:149	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	5	43	theme	hydrophilic	788:798	arg1	chromatography					812:825	hydrophilic interaction chromatography enrichment	788:836	hydrophilic interaction chromatography enrichment of glycopeptides	788:853	METHODS In this study, we comprehensively mapped the glycosylation patterns of human plasma-derived FV by combining multienzyme digestion, hydrophilic interaction chromatography enrichment of glycopeptides, and alternated fragmentation mass spectrometry analysis.
32310329	4	44	theme	affects	579:585	arg1	profile					546:552	The glycan profile	535:552	The glycan profile of FV, as well as how it affects the activity, stability, and immunogenicity,	535:630	The glycan profile of FV, as well as how it affects the activity, stability, and immunogenicity, remains unknown.
32310329	2	45	theme	plasma	372:377	arg1	infusions					346:354	infusions	346:354	infusions of fresh frozen plasma and blood platelets	346:397	The only treatment for the disease is infusions of fresh frozen plasma and blood platelets.
32310329	2	45	theme	plasma	372:377	arg1	treatment					317:325	The only treatment	308:325	The only treatment for the disease	308:341	The only treatment for the disease is infusions of fresh frozen plasma and blood platelets.
32310329	8	46	theme	glycosylation	1257:1269	arg1	patterns					1271:1278	glycosylation patterns	1257:1278	glycosylation patterns of proteins with more than 2000 amino acids	1257:1322	In addition, the established methodology can be readily applied to analyze glycosylation patterns of proteins with more than 2000 amino acids.
32310329	4	47	theme	glycan	539:544	arg1	profile					546:552	The glycan profile	535:552	The glycan profile of FV, as well as how it affects the activity, stability, and immunogenicity,	535:630	The glycan profile of FV, as well as how it affects the activity, stability, and immunogenicity, remains unknown.
32310329	5	48	link	plasma-derived	734:747	arg1	FV					749:750	human plasma-derived FV	728:750	human plasma-derived FV	728:750	METHODS In this study, we comprehensively mapped the glycosylation patterns of human plasma-derived FV by combining multienzyme digestion, hydrophilic interaction chromatography enrichment of glycopeptides, and alternated fragmentation mass spectrometry analysis.
32310329	5	49	theme	interaction	800:810	arg1	chromatography					812:825	hydrophilic interaction chromatography enrichment	788:836	hydrophilic interaction chromatography enrichment of glycopeptides	788:853	METHODS In this study, we comprehensively mapped the glycosylation patterns of human plasma-derived FV by combining multienzyme digestion, hydrophilic interaction chromatography enrichment of glycopeptides, and alternated fragmentation mass spectrometry analysis.
32310329	2	50	theme	frozen	365:370	arg1	plasma					372:377	fresh frozen plasma	359:377	fresh frozen plasma	359:377	The only treatment for the disease is infusions of fresh frozen plasma and blood platelets.
32310329	0	51	theme	coagulation	54:64	arg1	V					116:116	human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V	48:116	human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV)	48:121	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	0	51	theme	coagulation	54:64	arg1	FV					119:120	FV	119:120	FV	119:120	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	8	52	gly	glycosylation	1257:1269	arg1	proteins					1283:1290	proteins	1283:1290	proteins with more than 2000 amino acids	1283:1322	In addition, the established methodology can be readily applied to analyze glycosylation patterns of proteins with more than 2000 amino acids.
32310329	2	53	theme	only	312:315	arg1	infusions					346:354	infusions	346:354	infusions of fresh frozen plasma and blood platelets	346:397	The only treatment for the disease is infusions of fresh frozen plasma and blood platelets.
32310329	2	53	theme	only	312:315	arg1	treatment					317:325	The only treatment	308:325	The only treatment for the disease	308:341	The only treatment for the disease is infusions of fresh frozen plasma and blood platelets.
32310329	0	54	theme	human	48:52	arg1	V					116:116	human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V	48:116	human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV)	48:121	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	0	54	theme	human	48:52	arg1	FV					119:120	FV	119:120	FV	119:120	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	7	55	dep	studies	1144:1150	arg1	development					1169:1179	development	1169:1179	development	1169:1179	Such glycosylation details are fundamental for future functional studies and therapeutics development.
32310329	0	56	gly	O-glycosylation	21:35	arg1	V					116:116	human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V	48:116	human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV)	48:121	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	0	56	gly	O-glycosylation	21:35	arg1	FV					119:120	FV	119:120	FV	119:120	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	3	57	from	activity	437:444	arg1	plasma					527:532	the plasma	523:532	the plasma	523:532	Glycosylation affects the biological activity, pharmacokinetics, immunogenicity, and in vivo clearance rate of proteins in the plasma.
32310329	5	58	theme	spectrometry	890:901	arg1	analysis					903:910	alternated fragmentation mass spectrometry analysis	860:910	alternated fragmentation mass spectrometry analysis	860:910	METHODS In this study, we comprehensively mapped the glycosylation patterns of human plasma-derived FV by combining multienzyme digestion, hydrophilic interaction chromatography enrichment of glycopeptides, and alternated fragmentation mass spectrometry analysis.
32310329	7	59	theme	functional	1133:1142	arg1	studies					1144:1150	future functional studies	1126:1150	future functional studies	1126:1150	Such glycosylation details are fundamental for future functional studies and therapeutics development.
32310329	6	60	gly	N-glycopeptides	953:967	arg2	N-glycopeptides					953:967	57 unique N-glycopeptides	943:967	57 unique N-glycopeptides	943:967	RESULTS/CONCLUSION A total of 57 unique N-glycopeptides and 51 O-glycopeptides were identified, which were categorized into 40 N-glycan and 17 O-glycan compositions.
32310329	6	61	gly	O-glycopeptides	976:990	arg2	O-glycopeptides					976:990	51 O-glycopeptides	973:990	51 O-glycopeptides	973:990	RESULTS/CONCLUSION A total of 57 unique N-glycopeptides and 51 O-glycopeptides were identified, which were categorized into 40 N-glycan and 17 O-glycan compositions.
32310329	1	62	theme	FV	208:209	arg1	disorder					241:248	a rare bleeding disorder	225:248	a rare bleeding disorder that results in poor clotting after an injury or surgery	225:305	FV deficiency is a rare bleeding disorder that results in poor clotting after an injury or surgery.
32310329	1	62	theme	FV	208:209	arg1	deficiency					211:220	FV deficiency	208:220	FV deficiency	208:220	FV deficiency is a rare bleeding disorder that results in poor clotting after an injury or surgery.
32310329	6	63	theme	O-glycopeptides	976:990	arg1	total					934:938	A total	932:938	A total of 57 unique N-glycopeptides and 51 O-glycopeptides	932:990	RESULTS/CONCLUSION A total of 57 unique N-glycopeptides and 51 O-glycopeptides were identified, which were categorized into 40 N-glycan and 17 O-glycan compositions.
32310329	0	64	theme	V.	73:74	arg1	V					116:116	human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V	48:116	human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV)	48:121	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	0	64	theme	V.	73:74	arg1	FV					119:120	FV	119:120	FV	119:120	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	3	65	theme	biological	426:435	arg1	activity					437:444	the biological activity	422:444	the biological activity	422:444	Glycosylation affects the biological activity, pharmacokinetics, immunogenicity, and in vivo clearance rate of proteins in the plasma.
32310329	0	66	dep	N-	14:15	arg1	mapping					37:43	mapping	37:43	mapping	37:43	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	0	67	gly	glycoprotein	138:149	arg1	N-					14:15	Comprehensive N-	0:15	Comprehensive N-	0:15	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	0	67	gly	glycoprotein	138:149	arg1	glycoprotein					138:149	a multidomain glycoprotein	124:149	a multidomain glycoprotein	124:149	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	0	68	theme	factor	66:71	arg1	V					116:116	human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V	48:116	human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV)	48:121	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	0	68	theme	factor	66:71	arg1	FV					119:120	FV	119:120	FV	119:120	Comprehensive N- and O-glycosylation mapping of human coagulation factor V. BACKGROUND/OBJECTIVE Coagulation factor V (FV), a multidomain glycoprotein, is an essential cofactor in the blood clotting cascade.
32310329	5	69	theme	multienzyme	765:775	arg1	digestion					777:785	multienzyme digestion	765:785	multienzyme digestion	765:785	METHODS In this study, we comprehensively mapped the glycosylation patterns of human plasma-derived FV by combining multienzyme digestion, hydrophilic interaction chromatography enrichment of glycopeptides, and alternated fragmentation mass spectrometry analysis.
32310329	3	70	theme	clearance	493:501	arg1	rate					503:506	in vivo clearance rate	485:506	in vivo clearance rate	485:506	Glycosylation affects the biological activity, pharmacokinetics, immunogenicity, and in vivo clearance rate of proteins in the plasma.
32310329	3	71	from	rate	503:506	arg1	plasma					527:532	the plasma	523:532	the plasma	523:532	Glycosylation affects the biological activity, pharmacokinetics, immunogenicity, and in vivo clearance rate of proteins in the plasma.
33174834	4	0	theme	cerasi	664:669	arg1	203383T					676:682	Ornithinimicrobium cerasi CPCC 203383T	645:682	Ornithinimicrobium cerasi CPCC 203383T (96.6 %)	645:691	16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %).
33174834	4	0	theme	cerasi	664:669	arg1	%					690:690	96.6 %	685:690	96.6 %	685:690	16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %).
33174834	12	1	theme	1.1391T=KCTC	1593:1604	arg1	W204T					1579:1583	W204T	1579:1583	W204T (=GDMCC 1.1391T=KCTC 49237T)	1579:1612	The type strain is W204T (=GDMCC 1.1391T=KCTC 49237T).
33174834	12	1	theme	1.1391T=KCTC	1593:1604	arg1	49237T					1606:1611	=GDMCC 1.1391T=KCTC 49237T	1586:1611	=GDMCC 1.1391T=KCTC 49237T	1586:1611	The type strain is W204T (=GDMCC 1.1391T=KCTC 49237T).
33174834	6	2	theme	G+C	983:985	arg1	content					987:993	the genomic DNA G+C content	967:993	the genomic DNA G+C content	967:993	Complete genome analyses revealed that the size of the genome was 3.54 Mb and the genomic DNA G+C content was 70.79 mol%.
33174834	6	2	theme	G+C	983:985	arg1	%					1008:1008	70.79 mol%	999:1008	70.79 mol%	999:1008	Complete genome analyses revealed that the size of the genome was 3.54 Mb and the genomic DNA G+C content was 70.79 mol%.
33174834	2	3	theme	short-rod-shaped	102:117	arg1	strain					140:145	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain	65:145	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain	65:145	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain, W204T, was isolated from a soil sample collected from Jiadengyu national forest park in China and characterized using a polyphasic approach.
33174834	2	3	theme	short-rod-shaped	102:117	arg1	W204T					148:152	W204T	148:152	W204T	148:152	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain, W204T, was isolated from a soil sample collected from Jiadengyu national forest park in China and characterized using a polyphasic approach.
33174834	4	4	theme	Ornithinimicrobium	645:662	arg1	203383T					676:682	Ornithinimicrobium cerasi CPCC 203383T	645:682	Ornithinimicrobium cerasi CPCC 203383T (96.6 %)	645:691	16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %).
33174834	4	4	theme	Ornithinimicrobium	645:662	arg1	%					690:690	96.6 %	685:690	96.6 %	685:690	16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %).
33174834	4	5	dep	Ornithinimicrobium	452:469	arg1	flavum					471:476	flavum	471:476	flavum	471:476	16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %).
33174834	3	6	theme	diamino	355:361	arg1	ornithine					327:335	ornithine	327:335	ornithine	327:335	The cell-wall peptidoglycan contained ornithine as the diagnostic diamino acid.
33174834	3	6	theme	diamino	355:361	arg1	acid					363:366	the diagnostic diamino acid	340:366	the diagnostic diamino acid	340:366	The cell-wall peptidoglycan contained ornithine as the diagnostic diamino acid.
33174834	9	7	theme	predominant	1239:1249	arg1	 0					1286:1287	 0	1286:1287	 0	1286:1287	The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and C16 : 0.
33174834	9	7	theme	predominant	1239:1249	arg1	iso-C15 					1277:1284	iso-C15 	1277:1284	iso-C15 	1277:1284	The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and C16 : 0.
33174834	9	7	theme	predominant	1239:1249	arg1	acids					1266:1270	The predominant cellular fatty acids	1235:1270	The predominant cellular fatty acids	1235:1270	The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and C16 : 0.
33174834	10	8	theme	Ornithinimicrobium	1471:1488	arg1	species					1450:1456	a novel species	1442:1456	a novel species	1442:1456	The phenotypic, chemotaxonomic and phylogenetic data suggested that strain W204T should be classified as representative of a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium pratense sp.
33174834	4	9	theme	sequence	383:390	arg1	analysis					392:399	16S rRNA gene sequence analysis	369:399	16S rRNA gene sequence analysis	369:399	16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %).
33174834	2	10	theme	polyphasic	268:277	arg1	approach					279:286	a polyphasic approach	266:286	a polyphasic approach	266:286	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain, W204T, was isolated from a soil sample collected from Jiadengyu national forest park in China and characterized using a polyphasic approach.
33174834	9	11	theme	cellular	1251:1258	arg1	 0					1286:1287	 0	1286:1287	 0	1286:1287	The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and C16 : 0.
33174834	9	11	theme	cellular	1251:1258	arg1	iso-C15 					1277:1284	iso-C15 	1277:1284	iso-C15 	1277:1284	The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and C16 : 0.
33174834	9	11	theme	cellular	1251:1258	arg1	acids					1266:1270	The predominant cellular fatty acids	1235:1270	The predominant cellular fatty acids	1235:1270	The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and C16 : 0.
33174834	4	12	theme	kibberense	716:725	arg1	K22-20T					727:733	Ornithinimicrobium kibberense K22-20T	697:733	Ornithinimicrobium kibberense K22-20T (96.6 %)	697:742	16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %).
33174834	4	12	theme	kibberense	716:725	arg1	%					741:741	96.6 %	736:741	96.6 %	736:741	16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %).
33174834	4	13	theme	Serinicoccus	559:570	arg1	GP-T3-3T					582:589	Serinicoccus sediminis GP-T3-3T	559:589	Serinicoccus sediminis GP-T3-3T (96.8 %)	559:598	16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %).
33174834	4	13	theme	Serinicoccus	559:570	arg1	%					597:597	96.8 %	592:597	96.8 %	592:597	16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %).
33174834	4	14	theme	CPCC	478:481	arg1	203535T					483:489	Ornithinimicrobium flavum CPCC 203535T	452:489	Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity)	452:510	16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %).
33174834	7	15	theme	polar	1015:1019	arg1	profile					1027:1033	The polar lipid profile	1011:1033	The polar lipid profile	1011:1033	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid.
33174834	6	16	theme	DNA	979:981	arg1	content					987:993	the genomic DNA G+C content	967:993	the genomic DNA G+C content	967:993	Complete genome analyses revealed that the size of the genome was 3.54 Mb and the genomic DNA G+C content was 70.79 mol%.
33174834	6	16	theme	DNA	979:981	arg1	%					1008:1008	70.79 mol%	999:1008	70.79 mol%	999:1008	Complete genome analyses revealed that the size of the genome was 3.54 Mb and the genomic DNA G+C content was 70.79 mol%.
33174834	5	17	theme	strain	809:814	arg1	W204T					816:820	strain W204T	809:820	strain W204T	809:820	However, the digital DNA-DNA genome hybridization value between strain W204T and the closest related strain O. flavum CPCC 203535T was 21.90 %.
33174834	3	18	theme	diagnostic	344:353	arg1	ornithine					327:335	ornithine	327:335	ornithine	327:335	The cell-wall peptidoglycan contained ornithine as the diagnostic diamino acid.
33174834	3	18	theme	diagnostic	344:353	arg1	acid					363:366	the diagnostic diamino acid	340:366	the diagnostic diamino acid	340:366	The cell-wall peptidoglycan contained ornithine as the diagnostic diamino acid.
33174834	7	19	theme	lipid	1021:1025	arg1	profile					1027:1033	The polar lipid profile	1011:1033	The polar lipid profile	1011:1033	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid.
33174834	4	20	theme	16S	369:371	arg1	rRNA					373:376	16S rRNA	369:376	16S rRNA gene sequence analysis	369:399	16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %).
33174834	10	21	theme	pratense	1529:1536	arg1	sp					1538:1539	the name Ornithinimicrobium pratense sp	1501:1539	the name Ornithinimicrobium pratense sp	1501:1539	The phenotypic, chemotaxonomic and phylogenetic data suggested that strain W204T should be classified as representative of a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium pratense sp.
33174834	12	22	theme	=GDMCC	1586:1591	arg1	W204T					1579:1583	W204T	1579:1583	W204T (=GDMCC 1.1391T=KCTC 49237T)	1579:1612	The type strain is W204T (=GDMCC 1.1391T=KCTC 49237T).
33174834	12	22	theme	=GDMCC	1586:1591	arg1	49237T					1606:1611	=GDMCC 1.1391T=KCTC 49237T	1586:1611	=GDMCC 1.1391T=KCTC 49237T	1586:1611	The type strain is W204T (=GDMCC 1.1391T=KCTC 49237T).
33174834	4	23	theme	CGMCC	535:539	arg1	4.5582T					541:547	Serinicoccus profundi CGMCC 4.5582T	513:547	Serinicoccus profundi CGMCC 4.5582T (96.9 %)	513:556	16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %).
33174834	4	23	theme	CGMCC	535:539	arg1	%					555:555	96.9 %	550:555	96.9 %	550:555	16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %).
33174834	0	24	theme	pratense	19:26	arg1	sp					28:29	Ornithinimicrobium pratense sp	0:29	Ornithinimicrobium pratense sp.	0:30	Ornithinimicrobium pratense sp.
33174834	2	25	theme	bacterial	130:138	arg1	strain					140:145	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain	65:145	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain	65:145	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain, W204T, was isolated from a soil sample collected from Jiadengyu national forest park in China and characterized using a polyphasic approach.
33174834	2	25	theme	bacterial	130:138	arg1	W204T					148:152	W204T	148:152	W204T	148:152	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain, W204T, was isolated from a soil sample collected from Jiadengyu national forest park in China and characterized using a polyphasic approach.
33174834	10	26	theme	strain	1387:1392	arg1	representative					1424:1437	representative	1424:1437	representative of a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium pratense sp	1424:1539	The phenotypic, chemotaxonomic and phylogenetic data suggested that strain W204T should be classified as representative of a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium pratense sp.
33174834	10	26	theme	strain	1387:1392	arg1	W204T					1394:1398	strain W204T	1387:1398	strain W204T	1387:1398	The phenotypic, chemotaxonomic and phylogenetic data suggested that strain W204T should be classified as representative of a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium pratense sp.
33174834	9	27	dep	 0	1286:1287	arg1	 0					1315:1316	 0	1315:1316	 0	1315:1316	The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and C16 : 0.
33174834	9	27	dep	 0	1286:1287	arg1	C16 					1310:1313	C16 	1310:1313	C16 	1310:1313	The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and C16 : 0.
33174834	9	27	dep	 0	1286:1287	arg1	 0					1303:1304	 0	1303:1304	 0	1303:1304	The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and C16 : 0.
33174834	7	28	theme	unidentified	1118:1129	arg1	glycolipid					1131:1140	an unidentified glycolipid	1115:1140	an unidentified glycolipid	1115:1140	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid.
33174834	0	29	theme	Ornithinimicrobium	0:17	arg1	sp					28:29	Ornithinimicrobium pratense sp	0:29	Ornithinimicrobium pratense sp.	0:30	Ornithinimicrobium pratense sp.
33174834	4	30	theme	gene	378:381	arg1	analysis					392:399	16S rRNA gene sequence analysis	369:399	16S rRNA gene sequence analysis	369:399	16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %).
33174834	8	31	theme	major	1203:1207	arg1	MK-8					1225:1228	MK-8	1225:1228	MK-8(H4)	1225:1232	The major menaquinone was MK-8(H4).
33174834	8	31	theme	major	1203:1207	arg1	menaquinone					1209:1219	The major menaquinone	1199:1219	The major menaquinone	1199:1219	The major menaquinone was MK-8(H4).
33174834	5	32	theme	digital	758:764	arg1	hybridization					781:793	the digital DNA-DNA genome hybridization	754:793	the digital DNA-DNA genome hybridization value between strain W204T and the closest related strain O. flavum CPCC 203535T	754:874	However, the digital DNA-DNA genome hybridization value between strain W204T and the closest related strain O. flavum CPCC 203535T was 21.90 %.
33174834	10	33	theme	phenotypic	1323:1332	arg1	data					1367:1370	The phenotypic, chemotaxonomic and phylogenetic data	1319:1370	The phenotypic, chemotaxonomic and phylogenetic data	1319:1370	The phenotypic, chemotaxonomic and phylogenetic data suggested that strain W204T should be classified as representative of a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium pratense sp.
33174834	10	34	theme	phylogenetic	1354:1365	arg1	data					1367:1370	The phenotypic, chemotaxonomic and phylogenetic data	1319:1370	The phenotypic, chemotaxonomic and phylogenetic data	1319:1370	The phenotypic, chemotaxonomic and phylogenetic data suggested that strain W204T should be classified as representative of a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium pratense sp.
33174834	4	35	theme	rRNA	373:376	arg1	analysis					392:399	16S rRNA gene sequence analysis	369:399	16S rRNA gene sequence analysis	369:399	16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %).
33174834	4	36	theme	Ornithinimicrobium	697:714	arg1	K22-20T					727:733	Ornithinimicrobium kibberense K22-20T	697:733	Ornithinimicrobium kibberense K22-20T (96.6 %)	697:742	16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %).
33174834	4	36	theme	Ornithinimicrobium	697:714	arg1	%					741:741	96.6 %	736:741	96.6 %	736:741	16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %).
33174834	2	37	attach	isolated	159:166	arg2	W204T					148:152	W204T	148:152	W204T	148:152	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain, W204T, was isolated from a soil sample collected from Jiadengyu national forest park in China and characterized using a polyphasic approach.
33174834	2	37	attach	isolated	159:166	arg2	strain					140:145	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain	65:145	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain	65:145	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain, W204T, was isolated from a soil sample collected from Jiadengyu national forest park in China and characterized using a polyphasic approach.
33174834	2	37	attach	isolated	159:166	arg1	sample					180:185	a soil sample	173:185	a soil sample collected from Jiadengyu national forest park in China	173:240	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain, W204T, was isolated from a soil sample collected from Jiadengyu national forest park in China and characterized using a polyphasic approach.
33174834	5	38	theme	DNA-DNA	766:772	arg1	hybridization					781:793	the digital DNA-DNA genome hybridization	754:793	the digital DNA-DNA genome hybridization value between strain W204T and the closest related strain O. flavum CPCC 203535T	754:874	However, the digital DNA-DNA genome hybridization value between strain W204T and the closest related strain O. flavum CPCC 203535T was 21.90 %.
33174834	4	39	dep	203535T	483:489	arg1	similarity					500:509	similarity	500:509	similarity	500:509	16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %).
33174834	4	39	dep	203535T	483:489	arg1	%					497:497	97.4 %	492:497	97.4 %	492:497	16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %).
33174834	5	40	theme	O.	853:854	arg1	203535T					868:874	O. flavum CPCC 203535T	853:874	O. flavum CPCC 203535T	853:874	However, the digital DNA-DNA genome hybridization value between strain W204T and the closest related strain O. flavum CPCC 203535T was 21.90 %.
33174834	5	41	theme	hybridization	781:793	arg1	value					795:799	the digital DNA-DNA genome hybridization value	754:799	the digital DNA-DNA genome hybridization value between strain W204T and the closest related strain O. flavum CPCC 203535T	754:874	However, the digital DNA-DNA genome hybridization value between strain W204T and the closest related strain O. flavum CPCC 203535T was 21.90 %.
33174834	7	42	theme	unidentified	1146:1157	arg1	phospholipid					1159:1170	an unidentified phospholipid	1143:1170	an unidentified phospholipid	1143:1170	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid.
33174834	5	43	theme	CPCC	863:866	arg1	203535T					868:874	O. flavum CPCC 203535T	853:874	O. flavum CPCC 203535T	853:874	However, the digital DNA-DNA genome hybridization value between strain W204T and the closest related strain O. flavum CPCC 203535T was 21.90 %.
33174834	9	44	theme	fatty	1260:1264	arg1	 0					1286:1287	 0	1286:1287	 0	1286:1287	The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and C16 : 0.
33174834	9	44	theme	fatty	1260:1264	arg1	iso-C15 					1277:1284	iso-C15 	1277:1284	iso-C15 	1277:1284	The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and C16 : 0.
33174834	9	44	theme	fatty	1260:1264	arg1	acids					1266:1270	The predominant cellular fatty acids	1235:1270	The predominant cellular fatty acids	1235:1270	The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and C16 : 0.
33174834	4	45	dep	Serinicoccus	559:570	arg1	sediminis					572:580	sediminis	572:580	sediminis	572:580	16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %).
33174834	6	46	theme	genome	898:903	arg1	analyses					905:912	Complete genome analyses	889:912	Complete genome analyses	889:912	Complete genome analyses revealed that the size of the genome was 3.54 Mb and the genomic DNA G+C content was 70.79 mol%.
33174834	12	47	theme	type	1564:1567	arg1	W204T					1579:1583	W204T	1579:1583	W204T (=GDMCC 1.1391T=KCTC 49237T)	1579:1612	The type strain is W204T (=GDMCC 1.1391T=KCTC 49237T).
33174834	12	47	theme	type	1564:1567	arg1	strain					1569:1574	The type strain	1560:1574	The type strain	1560:1574	The type strain is W204T (=GDMCC 1.1391T=KCTC 49237T).
33174834	6	48	theme	genomic	971:977	arg1	content					987:993	the genomic DNA G+C content	967:993	the genomic DNA G+C content	967:993	Complete genome analyses revealed that the size of the genome was 3.54 Mb and the genomic DNA G+C content was 70.79 mol%.
33174834	6	48	theme	genomic	971:977	arg1	%					1008:1008	70.79 mol%	999:1008	70.79 mol%	999:1008	Complete genome analyses revealed that the size of the genome was 3.54 Mb and the genomic DNA G+C content was 70.79 mol%.
33174834	2	49	theme	yellow	94:99	arg1	strain					140:145	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain	65:145	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain	65:145	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain, W204T, was isolated from a soil sample collected from Jiadengyu national forest park in China and characterized using a polyphasic approach.
33174834	2	49	theme	yellow	94:99	arg1	W204T					148:152	W204T	148:152	W204T	148:152	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain, W204T, was isolated from a soil sample collected from Jiadengyu national forest park in China and characterized using a polyphasic approach.
33174834	10	50	theme	Ornithinimicrobium	1510:1527	arg1	sp					1538:1539	the name Ornithinimicrobium pratense sp	1501:1539	the name Ornithinimicrobium pratense sp	1501:1539	The phenotypic, chemotaxonomic and phylogenetic data suggested that strain W204T should be classified as representative of a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium pratense sp.
33174834	4	51	theme	profundi	526:533	arg1	4.5582T					541:547	Serinicoccus profundi CGMCC 4.5582T	513:547	Serinicoccus profundi CGMCC 4.5582T (96.9 %)	513:556	16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %).
33174834	4	51	theme	profundi	526:533	arg1	%					555:555	96.9 %	550:555	96.9 %	550:555	16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %).
33174834	10	52	theme	novel	1444:1448	arg1	species					1450:1456	a novel species	1442:1456	a novel species	1442:1456	The phenotypic, chemotaxonomic and phylogenetic data suggested that strain W204T should be classified as representative of a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium pratense sp.
33174834	4	53	theme	Serinicoccus	513:524	arg1	4.5582T					541:547	Serinicoccus profundi CGMCC 4.5582T	513:547	Serinicoccus profundi CGMCC 4.5582T (96.9 %)	513:556	16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %).
33174834	4	53	theme	Serinicoccus	513:524	arg1	%					555:555	96.9 %	550:555	96.9 %	550:555	16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %).
33174834	10	54	theme	species	1450:1456	arg1	representative					1424:1437	representative	1424:1437	representative of a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium pratense sp	1424:1539	The phenotypic, chemotaxonomic and phylogenetic data suggested that strain W204T should be classified as representative of a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium pratense sp.
33174834	10	54	theme	species	1450:1456	arg1	W204T					1394:1398	strain W204T	1387:1398	strain W204T	1387:1398	The phenotypic, chemotaxonomic and phylogenetic data suggested that strain W204T should be classified as representative of a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium pratense sp.
33174834	4	55	theme	Ornithinimicrobium	452:469	arg1	203535T					483:489	Ornithinimicrobium flavum CPCC 203535T	452:489	Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity)	452:510	16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %).
33174834	5	56	theme	flavum	856:861	arg1	203535T					868:874	O. flavum CPCC 203535T	853:874	O. flavum CPCC 203535T	853:874	However, the digital DNA-DNA genome hybridization value between strain W204T and the closest related strain O. flavum CPCC 203535T was 21.90 %.
33174834	2	57	theme	Gram-stain-positive	73:91	arg1	strain					140:145	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain	65:145	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain	65:145	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain, W204T, was isolated from a soil sample collected from Jiadengyu national forest park in China and characterized using a polyphasic approach.
33174834	2	57	theme	Gram-stain-positive	73:91	arg1	W204T					148:152	W204T	148:152	W204T	148:152	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain, W204T, was isolated from a soil sample collected from Jiadengyu national forest park in China and characterized using a polyphasic approach.
33174834	3	58	theme	cell-wall	293:301	arg1	peptidoglycan					303:315	The cell-wall peptidoglycan	289:315	The cell-wall peptidoglycan	289:315	The cell-wall peptidoglycan contained ornithine as the diagnostic diamino acid.
33174834	5	59	dep	W204T	816:820	arg1	203535T					868:874	O. flavum CPCC 203535T	853:874	O. flavum CPCC 203535T	853:874	However, the digital DNA-DNA genome hybridization value between strain W204T and the closest related strain O. flavum CPCC 203535T was 21.90 %.
33174834	4	60	theme	CPCC	671:674	arg1	203383T					676:682	Ornithinimicrobium cerasi CPCC 203383T	645:682	Ornithinimicrobium cerasi CPCC 203383T (96.6 %)	645:691	16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %).
33174834	4	60	theme	CPCC	671:674	arg1	%					690:690	96.6 %	685:690	96.6 %	685:690	16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %).
33174834	9	61	dep	iso-C15 	1277:1284	arg1	 0					1286:1287	 0	1286:1287	 0	1286:1287	The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and C16 : 0.
33174834	9	61	dep	iso-C15 	1277:1284	arg1	iso-C15 					1277:1284	iso-C15 	1277:1284	iso-C15 	1277:1284	The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and C16 : 0.
33174834	9	61	dep	iso-C15 	1277:1284	arg1	acids					1266:1270	The predominant cellular fatty acids	1235:1270	The predominant cellular fatty acids	1235:1270	The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and C16 : 0.
33174834	2	62	theme	novel	67:71	arg1	strain					140:145	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain	65:145	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain	65:145	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain, W204T, was isolated from a soil sample collected from Jiadengyu national forest park in China and characterized using a polyphasic approach.
33174834	2	62	theme	novel	67:71	arg1	W204T					148:152	W204T	148:152	W204T	148:152	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain, W204T, was isolated from a soil sample collected from Jiadengyu national forest park in China and characterized using a polyphasic approach.
33174834	6	63	theme	Complete	889:896	arg1	analyses					905:912	Complete genome analyses	889:912	Complete genome analyses	889:912	Complete genome analyses revealed that the size of the genome was 3.54 Mb and the genomic DNA G+C content was 70.79 mol%.
33174834	4	64	theme	strain	416:421	arg1	W204T					423:427	strain W204T	416:427	strain W204T	416:427	16S rRNA gene sequence analysis indicated that strain W204T was closely related to Ornithinimicrobium flavum CPCC 203535T (97.4 %, similarity), Serinicoccus profundi CGMCC 4.5582T (96.9 %), Serinicoccus sediminis GP-T3-3T (96.8 %), Serinicoccus hydrothermalis JLT9T (96.7 %), Ornithinimicrobium cerasi CPCC 203383T (96.6 %) and Ornithinimicrobium kibberense K22-20T (96.6 %).
33174834	10	65	theme	chemotaxonomic	1335:1348	arg1	data					1367:1370	The phenotypic, chemotaxonomic and phylogenetic data	1319:1370	The phenotypic, chemotaxonomic and phylogenetic data	1319:1370	The phenotypic, chemotaxonomic and phylogenetic data suggested that strain W204T should be classified as representative of a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium pratense sp.
33174834	2	66	theme	soil	175:178	arg1	sample					180:185	a soil sample	173:185	a soil sample collected from Jiadengyu national forest park in China	173:240	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain, W204T, was isolated from a soil sample collected from Jiadengyu national forest park in China and characterized using a polyphasic approach.
33174834	1	67	theme	meadow	52:57	arg1	soil					59:62	meadow soil	52:62	meadow soil	52:62	nov., isolated from meadow soil.
33174834	3	68	contain	contained	317:325	arg2	acid					363:366	the diagnostic diamino acid	340:366	the diagnostic diamino acid	340:366	The cell-wall peptidoglycan contained ornithine as the diagnostic diamino acid.
33174834	3	68	contain	contained	317:325	arg1	peptidoglycan					303:315	The cell-wall peptidoglycan	289:315	The cell-wall peptidoglycan	289:315	The cell-wall peptidoglycan contained ornithine as the diagnostic diamino acid.
33174834	3	68	contain	contained	317:325	arg2	ornithine					327:335	ornithine	327:335	ornithine	327:335	The cell-wall peptidoglycan contained ornithine as the diagnostic diamino acid.
33174834	7	69	theme	unidentified	1179:1190	arg1	lipid					1192:1196	an unidentified lipid	1176:1196	an unidentified lipid	1176:1196	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified glycolipid, an unidentified phospholipid and an unidentified lipid.
33174834	5	70	theme	genome	774:779	arg1	hybridization					781:793	the digital DNA-DNA genome hybridization	754:793	the digital DNA-DNA genome hybridization value between strain W204T and the closest related strain O. flavum CPCC 203535T	754:874	However, the digital DNA-DNA genome hybridization value between strain W204T and the closest related strain O. flavum CPCC 203535T was 21.90 %.
33174834	5	71	theme	closest	830:836	arg1	strain					846:851	the closest related strain	826:851	the closest related strain	826:851	However, the digital DNA-DNA genome hybridization value between strain W204T and the closest related strain O. flavum CPCC 203535T was 21.90 %.
33174834	6	72	theme	70.79 mol	999:1007	arg1	content					987:993	the genomic DNA G+C content	967:993	the genomic DNA G+C content	967:993	Complete genome analyses revealed that the size of the genome was 3.54 Mb and the genomic DNA G+C content was 70.79 mol%.
33174834	6	72	theme	70.79 mol	999:1007	arg1	%					1008:1008	70.79 mol%	999:1008	70.79 mol%	999:1008	Complete genome analyses revealed that the size of the genome was 3.54 Mb and the genomic DNA G+C content was 70.79 mol%.
33174834	10	73	theme	genus	1465:1469	arg1	Ornithinimicrobium					1471:1488	the genus Ornithinimicrobium	1461:1488	the genus Ornithinimicrobium	1461:1488	The phenotypic, chemotaxonomic and phylogenetic data suggested that strain W204T should be classified as representative of a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium pratense sp.
33174834	2	74	theme	coccoid	122:128	arg1	strain					140:145	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain	65:145	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain	65:145	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain, W204T, was isolated from a soil sample collected from Jiadengyu national forest park in China and characterized using a polyphasic approach.
33174834	2	74	theme	coccoid	122:128	arg1	W204T					148:152	W204T	148:152	W204T	148:152	A novel Gram-stain-positive, yellow, short-rod-shaped or coccoid bacterial strain, W204T, was isolated from a soil sample collected from Jiadengyu national forest park in China and characterized using a polyphasic approach.
33174834	10	75	theme	name	1505:1508	arg1	sp					1538:1539	the name Ornithinimicrobium pratense sp	1501:1539	the name Ornithinimicrobium pratense sp	1501:1539	The phenotypic, chemotaxonomic and phylogenetic data suggested that strain W204T should be classified as representative of a novel species of the genus Ornithinimicrobium, for which the name Ornithinimicrobium pratense sp.
33174834	5	76	theme	related	838:844	arg1	strain					846:851	the closest related strain	826:851	the closest related strain	826:851	However, the digital DNA-DNA genome hybridization value between strain W204T and the closest related strain O. flavum CPCC 203535T was 21.90 %.
33174834	6	77	theme	genome	944:949	arg1	3.54 Mb					955:961	3.54 Mb	955:961	3.54 Mb	955:961	Complete genome analyses revealed that the size of the genome was 3.54 Mb and the genomic DNA G+C content was 70.79 mol%.
33174834	6	77	theme	genome	944:949	arg1	size					932:935	the size	928:935	the size of the genome	928:949	Complete genome analyses revealed that the size of the genome was 3.54 Mb and the genomic DNA G+C content was 70.79 mol%.
32965208	2	0	theme	Gram-stain-positive	102:120	arg1	actinobacterium					158:172	A new Gram-stain-positive, aerobic, non-motile and rod-shaped actinobacterium	96:172	A new Gram-stain-positive, aerobic, non-motile and rod-shaped actinobacterium	96:172	A new Gram-stain-positive, aerobic, non-motile and rod-shaped actinobacterium, designated O1T, was isolated from a deep-sea sediment of the Western Pacific Ocean.
32965208	4	1	theme	fatty	465:469	arg1	acids					471:475	the major fatty acids	455:475	the major fatty acids	455:475	The predominant menaquinone was MK-8 (H2), and anteiso-C15 : 0 and anteiso-C17 : 0 were the major fatty acids.
32965208	4	1	theme	fatty	465:469	arg1	anteiso-C15 					414:425	anteiso-C15 	414:425	anteiso-C15 	414:425	The predominant menaquinone was MK-8 (H2), and anteiso-C15 : 0 and anteiso-C17 : 0 were the major fatty acids.
32965208	2	2	theme	new	98:100	arg1	actinobacterium					158:172	A new Gram-stain-positive, aerobic, non-motile and rod-shaped actinobacterium	96:172	A new Gram-stain-positive, aerobic, non-motile and rod-shaped actinobacterium	96:172	A new Gram-stain-positive, aerobic, non-motile and rod-shaped actinobacterium, designated O1T, was isolated from a deep-sea sediment of the Western Pacific Ocean.
32965208	5	3	theme	meso-diaminopimelic	541:559	arg1	acid					561:564	meso-diaminopimelic acid	541:564	meso-diaminopimelic acid	541:564	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
32965208	5	3	theme	meso-diaminopimelic	541:559	arg1	acid					501:504	The diagnostic diamino acid	478:504	The diagnostic diamino acid in the cell-wall peptidoglycan	478:535	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
32965208	10	4	theme	strain	1200:1205	arg1	O1T					1207:1209	strain O1T	1200:1209	strain O1T	1200:1209	On the basis of the morphological and chemotaxonomic characteristics, as well as the genotypic data, it is proposed that strain O1T represents a novel species of the genus Brevibacterium, for which the name Brevibacterium profundi sp.
32965208	4	5	theme	predominant	371:381	arg1	MK-8					399:402	MK-8	399:402	MK-8	399:402	The predominant menaquinone was MK-8 (H2), and anteiso-C15 : 0 and anteiso-C17 : 0 were the major fatty acids.
32965208	4	5	theme	predominant	371:381	arg1	menaquinone					383:393	The predominant menaquinone	367:393	The predominant menaquinone	367:393	The predominant menaquinone was MK-8 (H2), and anteiso-C15 : 0 and anteiso-C17 : 0 were the major fatty acids.
32965208	9	6	theme	DNA-DNA	940:946	arg1	hybridization					948:960	in silico DNA-DNA hybridization	930:960	in silico DNA-DNA hybridization	930:960	The values of average nucleotide identity and in silico DNA-DNA hybridization between strain O1T and its close relatives were well below the thresholds used for the delineation of a new species.
32965208	4	7	theme	major	459:463	arg1	acids					471:475	the major fatty acids	455:475	the major fatty acids	455:475	The predominant menaquinone was MK-8 (H2), and anteiso-C15 : 0 and anteiso-C17 : 0 were the major fatty acids.
32965208	4	7	theme	major	459:463	arg1	anteiso-C15 					414:425	anteiso-C15 	414:425	anteiso-C15 	414:425	The predominant menaquinone was MK-8 (H2), and anteiso-C15 : 0 and anteiso-C17 : 0 were the major fatty acids.
32965208	10	8	theme	Brevibacterium	1286:1299	arg1	sp					1310:1311	the name Brevibacterium profundi sp	1277:1311	the name Brevibacterium profundi sp	1277:1311	On the basis of the morphological and chemotaxonomic characteristics, as well as the genotypic data, it is proposed that strain O1T represents a novel species of the genus Brevibacterium, for which the name Brevibacterium profundi sp.
32965208	5	9	theme	diagnostic	482:491	arg1	acid					561:564	meso-diaminopimelic acid	541:564	meso-diaminopimelic acid	541:564	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
32965208	5	9	theme	diagnostic	482:491	arg1	acid					501:504	The diagnostic diamino acid	478:504	The diagnostic diamino acid in the cell-wall peptidoglycan	478:535	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
32965208	1	10	theme	deep-sea	48:55	arg1	sediment					57:64	deep-sea sediment	48:64	deep-sea sediment of the Western Pacific Ocean	48:93	nov., isolated from deep-sea sediment of the Western Pacific Ocean.
32965208	9	11	theme	hybridization	948:960	arg1	values					888:893	The values	884:893	The values of average nucleotide identity and in silico DNA-DNA hybridization between strain O1T and its close relatives	884:1003	The values of average nucleotide identity and in silico DNA-DNA hybridization between strain O1T and its close relatives were well below the thresholds used for the delineation of a new species.
32965208	9	12	theme	close	989:993	arg1	relatives					995:1003	its close relatives	985:1003	its close relatives	985:1003	The values of average nucleotide identity and in silico DNA-DNA hybridization between strain O1T and its close relatives were well below the thresholds used for the delineation of a new species.
32965208	5	13	theme	diamino	493:499	arg1	acid					561:564	meso-diaminopimelic acid	541:564	meso-diaminopimelic acid	541:564	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
32965208	5	13	theme	diamino	493:499	arg1	acid					501:504	The diagnostic diamino acid	478:504	The diagnostic diamino acid in the cell-wall peptidoglycan	478:535	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
32965208	8	14	theme	similarity	760:769	arg1	search					771:776	similarity search	760:776	similarity search	760:776	Based on a similarity search and phylogenetic analysis of the 16S rRNA gene sequence, strain O1T belonged to the genus Brevibacterium.
32965208	2	15	theme	non-motile	132:141	arg1	actinobacterium					158:172	A new Gram-stain-positive, aerobic, non-motile and rod-shaped actinobacterium	96:172	A new Gram-stain-positive, aerobic, non-motile and rod-shaped actinobacterium	96:172	A new Gram-stain-positive, aerobic, non-motile and rod-shaped actinobacterium, designated O1T, was isolated from a deep-sea sediment of the Western Pacific Ocean.
32965208	10	16	theme	genotypic	1164:1172	arg1	data					1174:1177	the genotypic data	1160:1177	the genotypic data	1160:1177	On the basis of the morphological and chemotaxonomic characteristics, as well as the genotypic data, it is proposed that strain O1T represents a novel species of the genus Brevibacterium, for which the name Brevibacterium profundi sp.
32965208	8	17	theme	genus	862:866	arg1	Brevibacterium					868:881	the genus Brevibacterium	858:881	the genus Brevibacterium	858:881	Based on a similarity search and phylogenetic analysis of the 16S rRNA gene sequence, strain O1T belonged to the genus Brevibacterium.
32965208	9	18	theme	strain	970:975	arg1	O1T					977:979	strain O1T	970:979	strain O1T	970:979	The values of average nucleotide identity and in silico DNA-DNA hybridization between strain O1T and its close relatives were well below the thresholds used for the delineation of a new species.
32965208	2	19	theme	Ocean	252:256	arg1	sediment					220:227	a deep-sea sediment	209:227	a deep-sea sediment of the Western Pacific Ocean	209:256	A new Gram-stain-positive, aerobic, non-motile and rod-shaped actinobacterium, designated O1T, was isolated from a deep-sea sediment of the Western Pacific Ocean.
32965208	3	20	theme	NaCl	361:364	arg1	presence					337:344	the presence	333:344	the presence of 1-5 % (w/v) NaCl	333:364	Strain O1T showed optimal growth at 30 °C, between pH 6.0 and 8.0, and in the presence of 1-5 % (w/v) NaCl.
32965208	0	21	theme	profundi	15:22	arg1	sp					24:25	Brevibacterium profundi sp	0:25	Brevibacterium profundi sp.	0:26	Brevibacterium profundi sp.
32965208	2	22	theme	aerobic	123:129	arg1	actinobacterium					158:172	A new Gram-stain-positive, aerobic, non-motile and rod-shaped actinobacterium	96:172	A new Gram-stain-positive, aerobic, non-motile and rod-shaped actinobacterium	96:172	A new Gram-stain-positive, aerobic, non-motile and rod-shaped actinobacterium, designated O1T, was isolated from a deep-sea sediment of the Western Pacific Ocean.
32965208	5	23	theme	cell-wall	513:521	arg1	peptidoglycan					523:535	the cell-wall peptidoglycan	509:535	the cell-wall peptidoglycan	509:535	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
32965208	8	24	dep	search	771:776	arg1	a					758:758	a	758:758	a	758:758	Based on a similarity search and phylogenetic analysis of the 16S rRNA gene sequence, strain O1T belonged to the genus Brevibacterium.
32965208	1	25	theme	Western	73:79	arg1	Ocean					89:93	the Western Pacific Ocean	69:93	the Western Pacific Ocean	69:93	nov., isolated from deep-sea sediment of the Western Pacific Ocean.
32965208	0	26	theme	Brevibacterium	0:13	arg1	sp					24:25	Brevibacterium profundi sp	0:25	Brevibacterium profundi sp.	0:26	Brevibacterium profundi sp.
32965208	3	27	dep	%	353:353	arg1	w/v					356:358	w/v	356:358	w/v	356:358	Strain O1T showed optimal growth at 30 °C, between pH 6.0 and 8.0, and in the presence of 1-5 % (w/v) NaCl.
32965208	9	28	dep	in	930:931	arg1	silico					933:938	silico	933:938	silico	933:938	The values of average nucleotide identity and in silico DNA-DNA hybridization between strain O1T and its close relatives were well below the thresholds used for the delineation of a new species.
32965208	1	29	theme	Pacific	81:87	arg1	Ocean					89:93	the Western Pacific Ocean	69:93	the Western Pacific Ocean	69:93	nov., isolated from deep-sea sediment of the Western Pacific Ocean.
32965208	10	30	theme	profundi	1301:1308	arg1	sp					1310:1311	the name Brevibacterium profundi sp	1277:1311	the name Brevibacterium profundi sp	1277:1311	On the basis of the morphological and chemotaxonomic characteristics, as well as the genotypic data, it is proposed that strain O1T represents a novel species of the genus Brevibacterium, for which the name Brevibacterium profundi sp.
32965208	10	31	theme	morphological	1099:1111	arg1	characteristics					1132:1146	the morphological and chemotaxonomic characteristics	1095:1146	the morphological and chemotaxonomic characteristics	1095:1146	On the basis of the morphological and chemotaxonomic characteristics, as well as the genotypic data, it is proposed that strain O1T represents a novel species of the genus Brevibacterium, for which the name Brevibacterium profundi sp.
32965208	3	32	theme	Strain	259:264	arg1	O1T					266:268	Strain O1T	259:268	Strain O1T	259:268	Strain O1T showed optimal growth at 30 °C, between pH 6.0 and 8.0, and in the presence of 1-5 % (w/v) NaCl.
32965208	9	33	theme	species	1070:1076	arg1	delineation					1049:1059	the delineation	1045:1059	the delineation of a new species	1045:1076	The values of average nucleotide identity and in silico DNA-DNA hybridization between strain O1T and its close relatives were well below the thresholds used for the delineation of a new species.
32965208	1	34	theme	Ocean	89:93	arg1	sediment					57:64	deep-sea sediment	48:64	deep-sea sediment of the Western Pacific Ocean	48:93	nov., isolated from deep-sea sediment of the Western Pacific Ocean.
32965208	6	35	theme	polar	577:581	arg1	diphosphatidylglycerol					595:616	diphosphatidylglycerol	595:616	diphosphatidylglycerol	595:616	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and one unknown glycolipid.
32965208	6	35	theme	polar	577:581	arg1	lipids					583:588	The major polar lipids	567:588	The major polar lipids	567:588	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and one unknown glycolipid.
32965208	10	36	theme	novel	1224:1228	arg1	species					1230:1236	a novel species	1222:1236	a novel species	1222:1236	On the basis of the morphological and chemotaxonomic characteristics, as well as the genotypic data, it is proposed that strain O1T represents a novel species of the genus Brevibacterium, for which the name Brevibacterium profundi sp.
32965208	10	37	theme	Brevibacterium	1251:1264	arg1	species					1230:1236	a novel species	1222:1236	a novel species	1222:1236	On the basis of the morphological and chemotaxonomic characteristics, as well as the genotypic data, it is proposed that strain O1T represents a novel species of the genus Brevibacterium, for which the name Brevibacterium profundi sp.
32965208	6	38	theme	major	571:575	arg1	diphosphatidylglycerol					595:616	diphosphatidylglycerol	595:616	diphosphatidylglycerol	595:616	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and one unknown glycolipid.
32965208	6	38	theme	major	571:575	arg1	lipids					583:588	The major polar lipids	567:588	The major polar lipids	567:588	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and one unknown glycolipid.
32965208	3	39	theme	1-5 	349:352	arg1	%					353:353	%	353:353	%	353:353	Strain O1T showed optimal growth at 30 °C, between pH 6.0 and 8.0, and in the presence of 1-5 % (w/v) NaCl.
32965208	7	40	theme	64.9 mol	706:713	arg1	content					680:686	The DNA G+C content	668:686	The DNA G+C content of strain O1T	668:700	The DNA G+C content of strain O1T was 64.9 mol% and the genome size was 4.17 Mb.
32965208	7	40	theme	64.9 mol	706:713	arg1	%					714:714	64.9 mol%	706:714	64.9 mol%	706:714	The DNA G+C content of strain O1T was 64.9 mol% and the genome size was 4.17 Mb.
32965208	12	41	theme	type	1336:1339	arg1	O1T					1351:1353	O1T	1351:1353	O1T (=JCM 33845T=MCCC 1A16744T)	1351:1381	The type strain is O1T (=JCM 33845T=MCCC 1A16744T).
32965208	12	41	theme	type	1336:1339	arg1	strain					1341:1346	The type strain	1332:1346	The type strain	1332:1346	The type strain is O1T (=JCM 33845T=MCCC 1A16744T).
32965208	7	42	theme	genome	724:729	arg1	size					731:734	the genome size	720:734	the genome size	720:734	The DNA G+C content of strain O1T was 64.9 mol% and the genome size was 4.17 Mb.
32965208	7	42	theme	genome	724:729	arg1	4.17 Mb					740:746	4.17 Mb	740:746	4.17 Mb	740:746	The DNA G+C content of strain O1T was 64.9 mol% and the genome size was 4.17 Mb.
32965208	3	43	theme	%	353:353	arg1	NaCl					361:364	1-5 % (w/v) NaCl	349:364	1-5 % (w/v) NaCl	349:364	Strain O1T showed optimal growth at 30 °C, between pH 6.0 and 8.0, and in the presence of 1-5 % (w/v) NaCl.
32965208	4	44	dep	anteiso-C15 	414:425	arg1	 0					447:448	 0	447:448	 0	447:448	The predominant menaquinone was MK-8 (H2), and anteiso-C15 : 0 and anteiso-C17 : 0 were the major fatty acids.
32965208	4	44	dep	anteiso-C15 	414:425	arg1	anteiso-C17 					434:445	anteiso-C17 	434:445	anteiso-C17 	434:445	The predominant menaquinone was MK-8 (H2), and anteiso-C15 : 0 and anteiso-C17 : 0 were the major fatty acids.
32965208	4	44	dep	anteiso-C15 	414:425	arg1	 0					427:428	 0	427:428	 0	427:428	The predominant menaquinone was MK-8 (H2), and anteiso-C15 : 0 and anteiso-C17 : 0 were the major fatty acids.
32965208	12	45	theme	33845T=MCCC	1361:1371	arg1	O1T					1351:1353	O1T	1351:1353	O1T (=JCM 33845T=MCCC 1A16744T)	1351:1381	The type strain is O1T (=JCM 33845T=MCCC 1A16744T).
32965208	12	45	theme	33845T=MCCC	1361:1371	arg1	1A16744T					1373:1380	=JCM 33845T=MCCC 1A16744T	1356:1380	=JCM 33845T=MCCC 1A16744T	1356:1380	The type strain is O1T (=JCM 33845T=MCCC 1A16744T).
32965208	12	46	theme	=JCM	1356:1359	arg1	O1T					1351:1353	O1T	1351:1353	O1T (=JCM 33845T=MCCC 1A16744T)	1351:1381	The type strain is O1T (=JCM 33845T=MCCC 1A16744T).
32965208	12	46	theme	=JCM	1356:1359	arg1	1A16744T					1373:1380	=JCM 33845T=MCCC 1A16744T	1356:1380	=JCM 33845T=MCCC 1A16744T	1356:1380	The type strain is O1T (=JCM 33845T=MCCC 1A16744T).
32965208	2	47	theme	deep-sea	211:218	arg1	sediment					220:227	a deep-sea sediment	209:227	a deep-sea sediment of the Western Pacific Ocean	209:256	A new Gram-stain-positive, aerobic, non-motile and rod-shaped actinobacterium, designated O1T, was isolated from a deep-sea sediment of the Western Pacific Ocean.
32965208	8	48	theme	rRNA	815:818	arg1	sequence					825:832	the 16S rRNA gene sequence	807:832	the 16S rRNA gene sequence	807:832	Based on a similarity search and phylogenetic analysis of the 16S rRNA gene sequence, strain O1T belonged to the genus Brevibacterium.
32965208	10	49	theme	name	1281:1284	arg1	sp					1310:1311	the name Brevibacterium profundi sp	1277:1311	the name Brevibacterium profundi sp	1277:1311	On the basis of the morphological and chemotaxonomic characteristics, as well as the genotypic data, it is proposed that strain O1T represents a novel species of the genus Brevibacterium, for which the name Brevibacterium profundi sp.
32965208	7	50	theme	strain	691:696	arg1	O1T					698:700	strain O1T	691:700	strain O1T	691:700	The DNA G+C content of strain O1T was 64.9 mol% and the genome size was 4.17 Mb.
32965208	9	51	theme	new	1066:1068	arg1	species					1070:1076	a new species	1064:1076	a new species	1064:1076	The values of average nucleotide identity and in silico DNA-DNA hybridization between strain O1T and its close relatives were well below the thresholds used for the delineation of a new species.
32965208	2	52	theme	rod-shaped	147:156	arg1	actinobacterium					158:172	A new Gram-stain-positive, aerobic, non-motile and rod-shaped actinobacterium	96:172	A new Gram-stain-positive, aerobic, non-motile and rod-shaped actinobacterium	96:172	A new Gram-stain-positive, aerobic, non-motile and rod-shaped actinobacterium, designated O1T, was isolated from a deep-sea sediment of the Western Pacific Ocean.
32965208	8	53	theme	16S	811:813	arg1	rRNA					815:818	16S rRNA	811:818	the 16S rRNA gene sequence	807:832	Based on a similarity search and phylogenetic analysis of the 16S rRNA gene sequence, strain O1T belonged to the genus Brevibacterium.
32965208	10	54	theme	genus	1245:1249	arg1	Brevibacterium					1251:1264	the genus Brevibacterium	1241:1264	the genus Brevibacterium	1241:1264	On the basis of the morphological and chemotaxonomic characteristics, as well as the genotypic data, it is proposed that strain O1T represents a novel species of the genus Brevibacterium, for which the name Brevibacterium profundi sp.
32965208	8	55	theme	sequence	825:832	arg1	search					771:776	similarity search	760:776	similarity search	760:776	Based on a similarity search and phylogenetic analysis of the 16S rRNA gene sequence, strain O1T belonged to the genus Brevibacterium.
32965208	8	55	theme	sequence	825:832	arg1	analysis					795:802	phylogenetic analysis	782:802	phylogenetic analysis	782:802	Based on a similarity search and phylogenetic analysis of the 16S rRNA gene sequence, strain O1T belonged to the genus Brevibacterium.
32965208	9	56	theme	average	898:904	arg1	identity					917:924	average nucleotide identity	898:924	average nucleotide identity	898:924	The values of average nucleotide identity and in silico DNA-DNA hybridization between strain O1T and its close relatives were well below the thresholds used for the delineation of a new species.
32965208	2	57	theme	Pacific	244:250	arg1	Ocean					252:256	the Western Pacific Ocean	232:256	the Western Pacific Ocean	232:256	A new Gram-stain-positive, aerobic, non-motile and rod-shaped actinobacterium, designated O1T, was isolated from a deep-sea sediment of the Western Pacific Ocean.
32965208	8	58	theme	gene	820:823	arg1	sequence					825:832	the 16S rRNA gene sequence	807:832	the 16S rRNA gene sequence	807:832	Based on a similarity search and phylogenetic analysis of the 16S rRNA gene sequence, strain O1T belonged to the genus Brevibacterium.
32965208	9	59	theme	nucleotide	906:915	arg1	identity					917:924	average nucleotide identity	898:924	average nucleotide identity	898:924	The values of average nucleotide identity and in silico DNA-DNA hybridization between strain O1T and its close relatives were well below the thresholds used for the delineation of a new species.
32965208	7	60	theme	DNA	672:674	arg1	content					680:686	The DNA G+C content	668:686	The DNA G+C content of strain O1T	668:700	The DNA G+C content of strain O1T was 64.9 mol% and the genome size was 4.17 Mb.
32965208	7	60	theme	DNA	672:674	arg1	%					714:714	64.9 mol%	706:714	64.9 mol%	706:714	The DNA G+C content of strain O1T was 64.9 mol% and the genome size was 4.17 Mb.
32965208	3	61	theme	optimal	277:283	arg1	growth					285:290	optimal growth	277:290	optimal growth	277:290	Strain O1T showed optimal growth at 30 °C, between pH 6.0 and 8.0, and in the presence of 1-5 % (w/v) NaCl.
32965208	2	62	theme	Western	236:242	arg1	Ocean					252:256	the Western Pacific Ocean	232:256	the Western Pacific Ocean	232:256	A new Gram-stain-positive, aerobic, non-motile and rod-shaped actinobacterium, designated O1T, was isolated from a deep-sea sediment of the Western Pacific Ocean.
32965208	8	63	theme	strain	835:840	arg1	O1T					842:844	strain O1T	835:844	strain O1T	835:844	Based on a similarity search and phylogenetic analysis of the 16S rRNA gene sequence, strain O1T belonged to the genus Brevibacterium.
32965208	8	64	theme	phylogenetic	782:793	arg1	analysis					795:802	phylogenetic analysis	782:802	phylogenetic analysis	782:802	Based on a similarity search and phylogenetic analysis of the 16S rRNA gene sequence, strain O1T belonged to the genus Brevibacterium.
32965208	9	65	theme	identity	917:924	arg1	values					888:893	The values	884:893	The values of average nucleotide identity and in silico DNA-DNA hybridization between strain O1T and its close relatives	884:1003	The values of average nucleotide identity and in silico DNA-DNA hybridization between strain O1T and its close relatives were well below the thresholds used for the delineation of a new species.
32965208	7	66	theme	G+C	676:678	arg1	content					680:686	The DNA G+C content	668:686	The DNA G+C content of strain O1T	668:700	The DNA G+C content of strain O1T was 64.9 mol% and the genome size was 4.17 Mb.
32965208	7	66	theme	G+C	676:678	arg1	%					714:714	64.9 mol%	706:714	64.9 mol%	706:714	The DNA G+C content of strain O1T was 64.9 mol% and the genome size was 4.17 Mb.
32965208	10	67	theme	chemotaxonomic	1117:1130	arg1	characteristics					1132:1146	the morphological and chemotaxonomic characteristics	1095:1146	the morphological and chemotaxonomic characteristics	1095:1146	On the basis of the morphological and chemotaxonomic characteristics, as well as the genotypic data, it is proposed that strain O1T represents a novel species of the genus Brevibacterium, for which the name Brevibacterium profundi sp.
32965208	6	68	theme	unknown	648:654	arg1	glycolipid					656:665	one unknown glycolipid	644:665	one unknown glycolipid	644:665	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and one unknown glycolipid.
32965208	2	69	attach	isolated	195:202	arg1	sediment					220:227	a deep-sea sediment	209:227	a deep-sea sediment of the Western Pacific Ocean	209:256	A new Gram-stain-positive, aerobic, non-motile and rod-shaped actinobacterium, designated O1T, was isolated from a deep-sea sediment of the Western Pacific Ocean.
32965208	2	69	attach	isolated	195:202	arg2	actinobacterium					158:172	A new Gram-stain-positive, aerobic, non-motile and rod-shaped actinobacterium	96:172	A new Gram-stain-positive, aerobic, non-motile and rod-shaped actinobacterium	96:172	A new Gram-stain-positive, aerobic, non-motile and rod-shaped actinobacterium, designated O1T, was isolated from a deep-sea sediment of the Western Pacific Ocean.
32965208	10	70	dep	characteristics	1132:1146	arg1	the					1082:1084	the	1082:1084	the	1082:1084	On the basis of the morphological and chemotaxonomic characteristics, as well as the genotypic data, it is proposed that strain O1T represents a novel species of the genus Brevibacterium, for which the name Brevibacterium profundi sp.
32965208	10	70	dep	characteristics	1132:1146	arg1	basis					1086:1090	basis	1086:1090	basis	1086:1090	On the basis of the morphological and chemotaxonomic characteristics, as well as the genotypic data, it is proposed that strain O1T represents a novel species of the genus Brevibacterium, for which the name Brevibacterium profundi sp.
32965208	5	71	from	acid	501:504	arg1	peptidoglycan					523:535	the cell-wall peptidoglycan	509:535	the cell-wall peptidoglycan	509:535	The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid.
32965208	9	72	theme	in	930:931	arg1	hybridization					948:960	in silico DNA-DNA hybridization	930:960	in silico DNA-DNA hybridization	930:960	The values of average nucleotide identity and in silico DNA-DNA hybridization between strain O1T and its close relatives were well below the thresholds used for the delineation of a new species.
32965208	7	73	theme	O1T	698:700	arg1	content					680:686	The DNA G+C content	668:686	The DNA G+C content of strain O1T	668:700	The DNA G+C content of strain O1T was 64.9 mol% and the genome size was 4.17 Mb.
32965208	7	73	theme	O1T	698:700	arg1	%					714:714	64.9 mol%	706:714	64.9 mol%	706:714	The DNA G+C content of strain O1T was 64.9 mol% and the genome size was 4.17 Mb.
31958766	5	0	theme	injury	729:734	arg1	models					714:719	The mice models	705:719	The mice models of lung injury	705:734	The mice models of lung injury were established and the changes of biochemical indices in serum and histopathology were detected to explore the effects of PDL.
31958766	5	1	from	changes	761:767	arg1	histopathology					805:818	histopathology	805:818	histopathology	805:818	The mice models of lung injury were established and the changes of biochemical indices in serum and histopathology were detected to explore the effects of PDL.
31958766	5	1	from	changes	761:767	arg1	serum					795:799	serum	795:799	serum	795:799	The mice models of lung injury were established and the changes of biochemical indices in serum and histopathology were detected to explore the effects of PDL.
31958766	7	2	theme	important	1085:1093	arg1	metabolites					1095:1105	the important metabolites	1081:1105	the important metabolites	1081:1105	Then network analysis was applied to visualize the protein related to the important metabolites, merging into a protein-metabolite network via Cytoscape.
31958766	4	3	theme	-induced	675:682	arg1	injury					689:694	lipopolysaccharide (LPS)-induced lung injury	651:694	lipopolysaccharide (LPS)-induced lung injury of mice	651:702	To identify the mechanism of the PDL in the treatment of lipopolysaccharide (LPS)-induced lung injury of mice.
31958766	5	4	from	indices	784:790	arg1	histopathology					805:818	histopathology	805:818	histopathology	805:818	The mice models of lung injury were established and the changes of biochemical indices in serum and histopathology were detected to explore the effects of PDL.
31958766	5	4	from	indices	784:790	arg1	serum					795:799	serum	795:799	serum	795:799	The mice models of lung injury were established and the changes of biochemical indices in serum and histopathology were detected to explore the effects of PDL.
31958766	6	5	theme	metabolic	955:963	arg1	pathways					965:972	the metabolic pathways	951:972	the metabolic pathways	951:972	The approach of GC-MS metabolomics was used to find more significant metabolites, and the metabolic pathways were enriched through MetaboAnalyst.
31958766	10	6	theme	potential	1543:1551	arg1	metabolites					1553:1563	potential metabolites	1543:1563	potential metabolites	1543:1563	The correlated network was constructed by integrating potential metabolites and pathways.
31958766	1	7	theme	anti-inflammatory	314:330	arg1	medicine					347:354	anti-inflammatory Chinese patent medicine	314:354	anti-inflammatory Chinese patent medicine recorded in Chinese Pharmacopeia	314:387	Pudilan Xiaoyan Oral Liquid (PDL) originated from "Pudilan" Classic Recipe of traditional Chinese medicine is one kind of anti-inflammatory Chinese patent medicine recorded in Chinese Pharmacopeia.
31958766	8	8	from	TNF-a	1321:1325	arg1	serum					1340:1344	serum	1340:1344	serum	1340:1344	The treatment of PDL could attenuate LPS-induced histopathological damage of lung tissues, followed by reducing pro-inflammation mediators including IL-10, TNF-a and NF-ĸB in serum.
31958766	12	9	theme	protein-metabolite	1891:1908	arg1	network					1910:1916	the disturbed protein-metabolite network	1877:1916	the disturbed protein-metabolite network	1877:1916	These results demonstrated that PDL might prevent the pathological process of lung injury through regulating the disturbed protein-metabolite network.
31958766	11	10	theme	potential	1707:1715	arg1	target					1717:1722	the potential target	1703:1722	the potential target to treat LPS-induced lung injury using PDL	1703:1765	Aspartate and l-cysteine were selected as key metabolites and correlated proteins such as IL4I1 and ASPA were speculated as the potential target to treat LPS-induced lung injury using PDL.
31958766	11	10	theme	potential	1707:1715	arg1	IL4I1					1669:1673	IL4I1	1669:1673	IL4I1	1669:1673	Aspartate and l-cysteine were selected as key metabolites and correlated proteins such as IL4I1 and ASPA were speculated as the potential target to treat LPS-induced lung injury using PDL.
31958766	11	10	theme	potential	1707:1715	arg1	ASPA					1679:1682	ASPA	1679:1682	ASPA	1679:1682	Aspartate and l-cysteine were selected as key metabolites and correlated proteins such as IL4I1 and ASPA were speculated as the potential target to treat LPS-induced lung injury using PDL.
31958766	11	10	theme	potential	1707:1715	arg1	proteins					1652:1659	correlated proteins	1641:1659	correlated proteins such as IL4I1 and ASPA	1641:1682	Aspartate and l-cysteine were selected as key metabolites and correlated proteins such as IL4I1 and ASPA were speculated as the potential target to treat LPS-induced lung injury using PDL.
31958766	12	11	theme	disturbed	1881:1889	arg1	network					1910:1916	the disturbed protein-metabolite network	1877:1916	the disturbed protein-metabolite network	1877:1916	These results demonstrated that PDL might prevent the pathological process of lung injury through regulating the disturbed protein-metabolite network.
31958766	0	12	theme	possible	95:102	arg1	mechanism					115:123	the possible protection mechanism	91:123	the possible protection mechanism of Pudilan Xiaoyan Oral Liquid in LPS-induced lung injury of mice	91:189	GC-MS based metabolomic profiling of lung tissue couple with network pharmacology revealed the possible protection mechanism of Pudilan Xiaoyan Oral Liquid in LPS-induced lung injury of mice.
31958766	1	13	theme	Chinese	332:338	arg1	medicine					347:354	anti-inflammatory Chinese patent medicine	314:354	anti-inflammatory Chinese patent medicine recorded in Chinese Pharmacopeia	314:387	Pudilan Xiaoyan Oral Liquid (PDL) originated from "Pudilan" Classic Recipe of traditional Chinese medicine is one kind of anti-inflammatory Chinese patent medicine recorded in Chinese Pharmacopeia.
31958766	1	14	theme	patent	340:345	arg1	medicine					347:354	anti-inflammatory Chinese patent medicine	314:354	anti-inflammatory Chinese patent medicine recorded in Chinese Pharmacopeia	314:387	Pudilan Xiaoyan Oral Liquid (PDL) originated from "Pudilan" Classic Recipe of traditional Chinese medicine is one kind of anti-inflammatory Chinese patent medicine recorded in Chinese Pharmacopeia.
31958766	0	15	theme	mice	186:189	arg1	injury					176:181	LPS-induced lung injury	159:181	LPS-induced lung injury of mice	159:189	GC-MS based metabolomic profiling of lung tissue couple with network pharmacology revealed the possible protection mechanism of Pudilan Xiaoyan Oral Liquid in LPS-induced lung injury of mice.
31958766	5	16	theme	PDL	860:862	arg1	effects					849:855	the effects	845:855	the effects of PDL	845:862	The mice models of lung injury were established and the changes of biochemical indices in serum and histopathology were detected to explore the effects of PDL.
31958766	1	17	theme	"	250:250	arg1	Recipe					260:265	"Pudilan" Classic Recipe	242:265	"Pudilan" Classic Recipe of traditional Chinese medicine	242:297	Pudilan Xiaoyan Oral Liquid (PDL) originated from "Pudilan" Classic Recipe of traditional Chinese medicine is one kind of anti-inflammatory Chinese patent medicine recorded in Chinese Pharmacopeia.
31958766	5	18	theme	mice	709:712	arg1	models					714:719	The mice models	705:719	The mice models of lung injury	705:734	The mice models of lung injury were established and the changes of biochemical indices in serum and histopathology were detected to explore the effects of PDL.
31958766	1	19	theme	medicine	347:354	arg1	kind					306:309	one kind	302:309	one kind of anti-inflammatory Chinese patent medicine recorded in Chinese Pharmacopeia	302:387	Pudilan Xiaoyan Oral Liquid (PDL) originated from "Pudilan" Classic Recipe of traditional Chinese medicine is one kind of anti-inflammatory Chinese patent medicine recorded in Chinese Pharmacopeia.
31958766	0	20	theme	protection	104:113	arg1	mechanism					115:123	the possible protection mechanism	91:123	the possible protection mechanism of Pudilan Xiaoyan Oral Liquid in LPS-induced lung injury of mice	91:189	GC-MS based metabolomic profiling of lung tissue couple with network pharmacology revealed the possible protection mechanism of Pudilan Xiaoyan Oral Liquid in LPS-induced lung injury of mice.
31958766	2	21	theme	tract	473:477	arg1	diseases					445:452	inflammatory diseases	432:452	inflammatory diseases of the respiratory tract	432:477	PDL has been used clinically for treating inflammatory diseases of the respiratory tract.
31958766	2	22	theme	inflammatory	432:443	arg1	diseases					445:452	inflammatory diseases	432:452	inflammatory diseases of the respiratory tract	432:477	PDL has been used clinically for treating inflammatory diseases of the respiratory tract.
31958766	8	23	theme	histopathological	1214:1230	arg1	damage					1232:1237	LPS-induced histopathological damage	1202:1237	LPS-induced histopathological damage of lung tissues	1202:1253	The treatment of PDL could attenuate LPS-induced histopathological damage of lung tissues, followed by reducing pro-inflammation mediators including IL-10, TNF-a and NF-ĸB in serum.
31958766	0	24	theme	based	6:10	arg1	profiling					24:32	GC-MS based metabolomic profiling	0:32	GC-MS based metabolomic profiling of lung tissue couple with network pharmacology	0:80	GC-MS based metabolomic profiling of lung tissue couple with network pharmacology revealed the possible protection mechanism of Pudilan Xiaoyan Oral Liquid in LPS-induced lung injury of mice.
31958766	1	25	theme	traditional	270:280	arg1	medicine					290:297	traditional Chinese medicine	270:297	traditional Chinese medicine	270:297	Pudilan Xiaoyan Oral Liquid (PDL) originated from "Pudilan" Classic Recipe of traditional Chinese medicine is one kind of anti-inflammatory Chinese patent medicine recorded in Chinese Pharmacopeia.
31958766	4	26	from	mechanism	610:618	arg1	treatment					638:646	the treatment	634:646	the treatment of lipopolysaccharide (LPS)-induced lung injury of mice	634:702	To identify the mechanism of the PDL in the treatment of lipopolysaccharide (LPS)-induced lung injury of mice.
31958766	8	27	theme	lung	1242:1245	arg1	tissues					1247:1253	lung tissues	1242:1253	lung tissues	1242:1253	The treatment of PDL could attenuate LPS-induced histopathological damage of lung tissues, followed by reducing pro-inflammation mediators including IL-10, TNF-a and NF-ĸB in serum.
31958766	0	28	theme	GC-MS	0:4	arg1	profiling					24:32	GC-MS based metabolomic profiling	0:32	GC-MS based metabolomic profiling of lung tissue couple with network pharmacology	0:80	GC-MS based metabolomic profiling of lung tissue couple with network pharmacology revealed the possible protection mechanism of Pudilan Xiaoyan Oral Liquid in LPS-induced lung injury of mice.
31958766	1	29	theme	Classic	252:258	arg1	Recipe					260:265	"Pudilan" Classic Recipe	242:265	"Pudilan" Classic Recipe of traditional Chinese medicine	242:297	Pudilan Xiaoyan Oral Liquid (PDL) originated from "Pudilan" Classic Recipe of traditional Chinese medicine is one kind of anti-inflammatory Chinese patent medicine recorded in Chinese Pharmacopeia.
31958766	11	30	theme	lung	1745:1748	arg1	injury					1750:1755	LPS-induced lung injury	1733:1755	LPS-induced lung injury	1733:1755	Aspartate and l-cysteine were selected as key metabolites and correlated proteins such as IL4I1 and ASPA were speculated as the potential target to treat LPS-induced lung injury using PDL.
31958766	5	31	theme	indices	784:790	arg1	changes					761:767	the changes	757:767	the changes of biochemical indices in serum and histopathology	757:818	The mice models of lung injury were established and the changes of biochemical indices in serum and histopathology were detected to explore the effects of PDL.
31958766	5	32	theme	biochemical	772:782	arg1	indices					784:790	biochemical indices	772:790	biochemical indices in serum and histopathology	772:818	The mice models of lung injury were established and the changes of biochemical indices in serum and histopathology were detected to explore the effects of PDL.
31958766	8	33	from	IL-10	1314:1318	arg1	serum					1340:1344	serum	1340:1344	serum	1340:1344	The treatment of PDL could attenuate LPS-induced histopathological damage of lung tissues, followed by reducing pro-inflammation mediators including IL-10, TNF-a and NF-ĸB in serum.
31958766	3	34	theme	potential	532:540	arg1	anti-inflammation					542:558	its potential anti-inflammation	528:558	its potential anti-inflammation	528:558	However, due to the complex composition of PDL, its potential anti-inflammation and the mechanism remain unknown.
31958766	12	35	theme	injury	1851:1856	arg1	process					1835:1841	the pathological process	1818:1841	the pathological process of lung injury	1818:1856	These results demonstrated that PDL might prevent the pathological process of lung injury through regulating the disturbed protein-metabolite network.
31958766	0	36	theme	metabolomic	12:22	arg1	profiling					24:32	GC-MS based metabolomic profiling	0:32	GC-MS based metabolomic profiling of lung tissue couple with network pharmacology	0:80	GC-MS based metabolomic profiling of lung tissue couple with network pharmacology revealed the possible protection mechanism of Pudilan Xiaoyan Oral Liquid in LPS-induced lung injury of mice.
31958766	5	37	from	serum	795:799	arg1	changes					761:767	the changes	757:767	the changes of biochemical indices in serum and histopathology	757:818	The mice models of lung injury were established and the changes of biochemical indices in serum and histopathology were detected to explore the effects of PDL.
31958766	1	38	theme	Chinese	368:374	arg1	Pharmacopeia					376:387	Chinese Pharmacopeia	368:387	Chinese Pharmacopeia	368:387	Pudilan Xiaoyan Oral Liquid (PDL) originated from "Pudilan" Classic Recipe of traditional Chinese medicine is one kind of anti-inflammatory Chinese patent medicine recorded in Chinese Pharmacopeia.
31958766	8	39	theme	tissues	1247:1253	arg1	damage					1232:1237	LPS-induced histopathological damage	1202:1237	LPS-induced histopathological damage of lung tissues	1202:1253	The treatment of PDL could attenuate LPS-induced histopathological damage of lung tissues, followed by reducing pro-inflammation mediators including IL-10, TNF-a and NF-ĸB in serum.
31958766	0	40	theme	lung	37:40	arg1	tissue					42:47	lung tissue	37:47	lung tissue	37:47	GC-MS based metabolomic profiling of lung tissue couple with network pharmacology revealed the possible protection mechanism of Pudilan Xiaoyan Oral Liquid in LPS-induced lung injury of mice.
31958766	0	41	from	injury	176:181	arg1	mechanism					115:123	the possible protection mechanism	91:123	the possible protection mechanism of Pudilan Xiaoyan Oral Liquid in LPS-induced lung injury of mice	91:189	GC-MS based metabolomic profiling of lung tissue couple with network pharmacology revealed the possible protection mechanism of Pudilan Xiaoyan Oral Liquid in LPS-induced lung injury of mice.
31958766	6	42	used	used	904:907	arg2	approach					869:876	The approach	865:876	The approach of GC-MS metabolomics	865:898	The approach of GC-MS metabolomics was used to find more significant metabolites, and the metabolic pathways were enriched through MetaboAnalyst.
31958766	3	43	theme	PDL	523:525	arg1	composition					508:518	the complex composition	496:518	the complex composition of PDL	496:525	However, due to the complex composition of PDL, its potential anti-inflammation and the mechanism remain unknown.
31958766	9	44	theme	PDL	1474:1476	arg1	treatment					1478:1486	PDL treatment	1474:1486	PDL treatment	1474:1486	11 potential metabolites were identified in lung tissue through metabolomics, which were significantly regulated to recover by PDL treatment.
31958766	2	45	theme	respiratory	461:471	arg1	tract					473:477	the respiratory tract	457:477	the respiratory tract	457:477	PDL has been used clinically for treating inflammatory diseases of the respiratory tract.
31958766	1	46	theme	Chinese	282:288	arg1	medicine					290:297	traditional Chinese medicine	270:297	traditional Chinese medicine	270:297	Pudilan Xiaoyan Oral Liquid (PDL) originated from "Pudilan" Classic Recipe of traditional Chinese medicine is one kind of anti-inflammatory Chinese patent medicine recorded in Chinese Pharmacopeia.
31958766	0	47	theme	Xiaoyan	136:142	arg1	mechanism					115:123	the possible protection mechanism	91:123	the possible protection mechanism of Pudilan Xiaoyan Oral Liquid in LPS-induced lung injury of mice	91:189	GC-MS based metabolomic profiling of lung tissue couple with network pharmacology revealed the possible protection mechanism of Pudilan Xiaoyan Oral Liquid in LPS-induced lung injury of mice.
31958766	7	48	theme	merging	1108:1114	arg1	metabolites					1095:1105	the important metabolites	1081:1105	the important metabolites	1081:1105	Then network analysis was applied to visualize the protein related to the important metabolites, merging into a protein-metabolite network via Cytoscape.
31958766	7	49	theme	protein-metabolite	1123:1140	arg1	network					1142:1148	a protein-metabolite network	1121:1148	a protein-metabolite network via Cytoscape	1121:1162	Then network analysis was applied to visualize the protein related to the important metabolites, merging into a protein-metabolite network via Cytoscape.
31958766	5	50	from	histopathology	805:818	arg1	changes					761:767	the changes	757:767	the changes of biochemical indices in serum and histopathology	757:818	The mice models of lung injury were established and the changes of biochemical indices in serum and histopathology were detected to explore the effects of PDL.
31958766	4	51	theme	PDL	627:629	arg1	mechanism					610:618	the mechanism	606:618	the mechanism of the PDL in the treatment of lipopolysaccharide (LPS)-induced lung injury of mice	606:702	To identify the mechanism of the PDL in the treatment of lipopolysaccharide (LPS)-induced lung injury of mice.
31958766	8	52	theme	pro-inflammation	1277:1292	arg1	mediators					1294:1302	pro-inflammation mediators	1277:1302	pro-inflammation mediators including IL-10, TNF-a and NF-ĸB in serum	1277:1344	The treatment of PDL could attenuate LPS-induced histopathological damage of lung tissues, followed by reducing pro-inflammation mediators including IL-10, TNF-a and NF-ĸB in serum.
31958766	8	52	theme	pro-inflammation	1277:1292	arg1	NF-ĸB					1331:1335	NF-ĸB	1331:1335	NF-ĸB	1331:1335	The treatment of PDL could attenuate LPS-induced histopathological damage of lung tissues, followed by reducing pro-inflammation mediators including IL-10, TNF-a and NF-ĸB in serum.
31958766	8	52	theme	pro-inflammation	1277:1292	arg1	TNF-a					1321:1325	TNF-a	1321:1325	TNF-a	1321:1325	The treatment of PDL could attenuate LPS-induced histopathological damage of lung tissues, followed by reducing pro-inflammation mediators including IL-10, TNF-a and NF-ĸB in serum.
31958766	8	52	theme	pro-inflammation	1277:1292	arg1	IL-10					1314:1318	IL-10	1314:1318	IL-10	1314:1318	The treatment of PDL could attenuate LPS-induced histopathological damage of lung tissues, followed by reducing pro-inflammation mediators including IL-10, TNF-a and NF-ĸB in serum.
31958766	1	53	theme	medicine	290:297	arg1	Recipe					260:265	"Pudilan" Classic Recipe	242:265	"Pudilan" Classic Recipe of traditional Chinese medicine	242:297	Pudilan Xiaoyan Oral Liquid (PDL) originated from "Pudilan" Classic Recipe of traditional Chinese medicine is one kind of anti-inflammatory Chinese patent medicine recorded in Chinese Pharmacopeia.
31958766	8	54	theme	PDL	1182:1184	arg1	treatment					1169:1177	The treatment	1165:1177	The treatment of PDL	1165:1184	The treatment of PDL could attenuate LPS-induced histopathological damage of lung tissues, followed by reducing pro-inflammation mediators including IL-10, TNF-a and NF-ĸB in serum.
31958766	6	55	theme	significant	922:932	arg1	metabolites					934:944	more significant metabolites	917:944	more significant metabolites	917:944	The approach of GC-MS metabolomics was used to find more significant metabolites, and the metabolic pathways were enriched through MetaboAnalyst.
31958766	11	56	theme	key	1621:1623	arg1	Aspartate					1579:1587	Aspartate	1579:1587	Aspartate	1579:1587	Aspartate and l-cysteine were selected as key metabolites and correlated proteins such as IL4I1 and ASPA were speculated as the potential target to treat LPS-induced lung injury using PDL.
31958766	11	56	theme	key	1621:1623	arg1	metabolites					1625:1635	key metabolites	1621:1635	key metabolites	1621:1635	Aspartate and l-cysteine were selected as key metabolites and correlated proteins such as IL4I1 and ASPA were speculated as the potential target to treat LPS-induced lung injury using PDL.
31958766	11	56	theme	key	1621:1623	arg1	l-cysteine					1593:1602	l-cysteine	1593:1602	l-cysteine	1593:1602	Aspartate and l-cysteine were selected as key metabolites and correlated proteins such as IL4I1 and ASPA were speculated as the potential target to treat LPS-induced lung injury using PDL.
31958766	4	57	theme	injury	689:694	arg1	treatment					638:646	the treatment	634:646	the treatment of lipopolysaccharide (LPS)-induced lung injury of mice	634:702	To identify the mechanism of the PDL in the treatment of lipopolysaccharide (LPS)-induced lung injury of mice.
31958766	0	58	theme	tissue	42:47	arg1	profiling					24:32	GC-MS based metabolomic profiling	0:32	GC-MS based metabolomic profiling of lung tissue couple with network pharmacology	0:80	GC-MS based metabolomic profiling of lung tissue couple with network pharmacology revealed the possible protection mechanism of Pudilan Xiaoyan Oral Liquid in LPS-induced lung injury of mice.
31958766	7	59	theme	network	1016:1022	arg1	analysis					1024:1031	Then network analysis	1011:1031	Then network analysis	1011:1031	Then network analysis was applied to visualize the protein related to the important metabolites, merging into a protein-metabolite network via Cytoscape.
31958766	0	60	from	mechanism	115:123	arg1	injury					176:181	LPS-induced lung injury	159:181	LPS-induced lung injury of mice	159:189	GC-MS based metabolomic profiling of lung tissue couple with network pharmacology revealed the possible protection mechanism of Pudilan Xiaoyan Oral Liquid in LPS-induced lung injury of mice.
31958766	7	61	theme	related	1070:1076	arg1	protein					1062:1068	the protein	1058:1068	the protein related to the important metabolites, merging into a protein-metabolite network via Cytoscape	1058:1162	Then network analysis was applied to visualize the protein related to the important metabolites, merging into a protein-metabolite network via Cytoscape.
31958766	2	62	used	used	403:406	arg2	PDL					390:392	PDL	390:392	PDL	390:392	PDL has been used clinically for treating inflammatory diseases of the respiratory tract.
31958766	4	63	theme	lung	684:687	arg1	injury					689:694	lipopolysaccharide (LPS)-induced lung injury	651:694	lipopolysaccharide (LPS)-induced lung injury of mice	651:702	To identify the mechanism of the PDL in the treatment of lipopolysaccharide (LPS)-induced lung injury of mice.
31958766	6	64	theme	metabolomics	887:898	arg1	approach					869:876	The approach	865:876	The approach of GC-MS metabolomics	865:898	The approach of GC-MS metabolomics was used to find more significant metabolites, and the metabolic pathways were enriched through MetaboAnalyst.
31958766	0	65	theme	lung	171:174	arg1	injury					176:181	LPS-induced lung injury	159:181	LPS-induced lung injury of mice	159:189	GC-MS based metabolomic profiling of lung tissue couple with network pharmacology revealed the possible protection mechanism of Pudilan Xiaoyan Oral Liquid in LPS-induced lung injury of mice.
31958766	0	66	dep	profiling	24:32	arg1	couple					49:54	couple	49:54	couple with network pharmacology	49:80	GC-MS based metabolomic profiling of lung tissue couple with network pharmacology revealed the possible protection mechanism of Pudilan Xiaoyan Oral Liquid in LPS-induced lung injury of mice.
31958766	12	67	theme	lung	1846:1849	arg1	injury					1851:1856	lung injury	1846:1856	lung injury	1846:1856	These results demonstrated that PDL might prevent the pathological process of lung injury through regulating the disturbed protein-metabolite network.
31958766	4	68	theme	mice	699:702	arg1	injury					689:694	lipopolysaccharide (LPS)-induced lung injury	651:694	lipopolysaccharide (LPS)-induced lung injury of mice	651:702	To identify the mechanism of the PDL in the treatment of lipopolysaccharide (LPS)-induced lung injury of mice.
31958766	10	69	theme	correlated	1493:1502	arg1	network					1504:1510	The correlated network	1489:1510	The correlated network	1489:1510	The correlated network was constructed by integrating potential metabolites and pathways.
31958766	0	70	theme	LPS-induced	159:169	arg1	injury					176:181	LPS-induced lung injury	159:181	LPS-induced lung injury of mice	159:189	GC-MS based metabolomic profiling of lung tissue couple with network pharmacology revealed the possible protection mechanism of Pudilan Xiaoyan Oral Liquid in LPS-induced lung injury of mice.
31958766	11	71	theme	correlated	1641:1650	arg1	target					1717:1722	the potential target	1703:1722	the potential target to treat LPS-induced lung injury using PDL	1703:1765	Aspartate and l-cysteine were selected as key metabolites and correlated proteins such as IL4I1 and ASPA were speculated as the potential target to treat LPS-induced lung injury using PDL.
31958766	11	71	theme	correlated	1641:1650	arg1	IL4I1					1669:1673	IL4I1	1669:1673	IL4I1	1669:1673	Aspartate and l-cysteine were selected as key metabolites and correlated proteins such as IL4I1 and ASPA were speculated as the potential target to treat LPS-induced lung injury using PDL.
31958766	11	71	theme	correlated	1641:1650	arg1	ASPA					1679:1682	ASPA	1679:1682	ASPA	1679:1682	Aspartate and l-cysteine were selected as key metabolites and correlated proteins such as IL4I1 and ASPA were speculated as the potential target to treat LPS-induced lung injury using PDL.
31958766	11	71	theme	correlated	1641:1650	arg1	proteins					1652:1659	correlated proteins	1641:1659	correlated proteins such as IL4I1 and ASPA	1641:1682	Aspartate and l-cysteine were selected as key metabolites and correlated proteins such as IL4I1 and ASPA were speculated as the potential target to treat LPS-induced lung injury using PDL.
31958766	8	72	theme	LPS-induced	1202:1212	arg1	damage					1232:1237	LPS-induced histopathological damage	1202:1237	LPS-induced histopathological damage of lung tissues	1202:1253	The treatment of PDL could attenuate LPS-induced histopathological damage of lung tissues, followed by reducing pro-inflammation mediators including IL-10, TNF-a and NF-ĸB in serum.
31958766	0	73	theme	network	61:67	arg1	pharmacology					69:80	network pharmacology	61:80	network pharmacology	61:80	GC-MS based metabolomic profiling of lung tissue couple with network pharmacology revealed the possible protection mechanism of Pudilan Xiaoyan Oral Liquid in LPS-induced lung injury of mice.
31958766	11	74	theme	LPS-induced	1733:1743	arg1	injury					1750:1755	LPS-induced lung injury	1733:1755	LPS-induced lung injury	1733:1755	Aspartate and l-cysteine were selected as key metabolites and correlated proteins such as IL4I1 and ASPA were speculated as the potential target to treat LPS-induced lung injury using PDL.
31958766	3	75	theme	complex	500:506	arg1	composition					508:518	the complex composition	496:518	the complex composition of PDL	496:525	However, due to the complex composition of PDL, its potential anti-inflammation and the mechanism remain unknown.
31958766	8	76	from	NF-ĸB	1331:1335	arg1	serum					1340:1344	serum	1340:1344	serum	1340:1344	The treatment of PDL could attenuate LPS-induced histopathological damage of lung tissues, followed by reducing pro-inflammation mediators including IL-10, TNF-a and NF-ĸB in serum.
31958766	9	77	theme	lung	1391:1394	arg1	tissue					1396:1401	lung tissue	1391:1401	lung tissue	1391:1401	11 potential metabolites were identified in lung tissue through metabolomics, which were significantly regulated to recover by PDL treatment.
31958766	5	78	theme	lung	724:727	arg1	injury					729:734	lung injury	724:734	lung injury	724:734	The mice models of lung injury were established and the changes of biochemical indices in serum and histopathology were detected to explore the effects of PDL.
31958766	6	79	theme	GC-MS	881:885	arg1	metabolomics					887:898	GC-MS metabolomics	881:898	GC-MS metabolomics	881:898	The approach of GC-MS metabolomics was used to find more significant metabolites, and the metabolic pathways were enriched through MetaboAnalyst.
31958766	12	80	theme	pathological	1822:1833	arg1	process					1835:1841	the pathological process	1818:1841	the pathological process of lung injury	1818:1856	These results demonstrated that PDL might prevent the pathological process of lung injury through regulating the disturbed protein-metabolite network.
31958766	9	81	theme	potential	1350:1358	arg1	metabolites					1360:1370	11 potential metabolites	1347:1370	11 potential metabolites	1347:1370	11 potential metabolites were identified in lung tissue through metabolomics, which were significantly regulated to recover by PDL treatment.
34762578	2	0	dep	aerobic	78:84	arg1	endospore-forming					120:136	endospore-forming	120:136	endospore-forming	120:136	Two aerobic, Gram-stain-positive, rod-shaped, endospore-forming, thermophilic bacterial strains, designated FJAT-54423T and FJAT-54424, were isolated from hyperthermophilic compost sampled in Shanxi Province, PR China.
34762578	2	0	dep	aerobic	78:84	arg1	rod-shaped					108:117	rod-shaped	108:117	rod-shaped	108:117	Two aerobic, Gram-stain-positive, rod-shaped, endospore-forming, thermophilic bacterial strains, designated FJAT-54423T and FJAT-54424, were isolated from hyperthermophilic compost sampled in Shanxi Province, PR China.
34762578	2	0	dep	aerobic	78:84	arg1	Gram-stain-positive					87:105	Gram-stain-positive	87:105	Gram-stain-positive	87:105	Two aerobic, Gram-stain-positive, rod-shaped, endospore-forming, thermophilic bacterial strains, designated FJAT-54423T and FJAT-54424, were isolated from hyperthermophilic compost sampled in Shanxi Province, PR China.
34762578	2	0	dep	aerobic	78:84	arg1	thermophilic					139:150	thermophilic	139:150	thermophilic	139:150	Two aerobic, Gram-stain-positive, rod-shaped, endospore-forming, thermophilic bacterial strains, designated FJAT-54423T and FJAT-54424, were isolated from hyperthermophilic compost sampled in Shanxi Province, PR China.
34762578	3	1	dep	optimum	358:364	arg1	pH					367:368	pH 7.0	367:372	pH 7.0	367:372	Growth was observed at 30-60 °C (optimum, 50 °C) and pH 6.0-9.0 (optimum, pH 7.0), with up to 2.0 % (w/v) NaCl (optimum, 0 % NaCl).
34762578	10	2	theme	related	1270:1276	arg1	strain					1288:1293	its most closely related reference strain	1253:1293	its most closely related reference strain	1253:1293	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	10	3	theme	digital	1183:1189	arg1	dDDH					1214:1217	dDDH	1214:1217	dDDH	1214:1217	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	10	3	theme	digital	1183:1189	arg1	hybridization					1199:1211	digital DNA-DNA hybridization	1183:1211	digital DNA-DNA hybridization (dDDH)	1183:1218	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	10	4	theme	average	1145:1151	arg1	ANI					1174:1176	ANI	1174:1176	ANI	1174:1176	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	10	4	theme	average	1145:1151	arg1	identity					1164:1171	average nucleotide identity	1145:1171	average nucleotide identity (ANI)	1145:1177	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	11	5	theme	ANI	1538:1540	arg1	values					1551:1556	ANI and dDDH values	1538:1556	ANI and dDDH values	1538:1556	Based on the observed physiological properties, chemotaxonomic characteristics and ANI and dDDH values, FJAT-54423T and FJAT-54424 belong to a novel species of the genus Brevibacillus, for which the name Brevibacillus composti sp.
34762578	11	6	theme	observed	1468:1475	arg1	properties					1491:1500	the observed physiological properties	1464:1500	the observed physiological properties	1464:1500	Based on the observed physiological properties, chemotaxonomic characteristics and ANI and dDDH values, FJAT-54423T and FJAT-54424 belong to a novel species of the genus Brevibacillus, for which the name Brevibacillus composti sp.
34762578	8	7	contain	contain	1010:1016	arg1	peptidoglycan					983:995	The cell-wall peptidoglycan	969:995	The cell-wall peptidoglycan	969:995	The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid.
34762578	8	7	contain	contain	1010:1016	arg2	acid					1038:1041	meso-diaminopimelic acid	1018:1041	meso-diaminopimelic acid	1018:1041	The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid.
34762578	13	8	theme	type	1708:1711	arg1	FJAT-54423T					1723:1733	FJAT-54423T	1723:1733	FJAT-54423T (=GDMCC 1.2054T=KCTC 43273T)	1723:1762	The type strain is FJAT-54423T (=GDMCC 1.2054T=KCTC 43273T).
34762578	13	8	theme	type	1708:1711	arg1	strain					1713:1718	The type strain	1704:1718	The type strain	1704:1718	The type strain is FJAT-54423T (=GDMCC 1.2054T=KCTC 43273T).
34762578	10	9	theme	dDDH	1443:1446	arg1	delineation					1403:1413	the recommended species delineation thresholds	1379:1424	the recommended species delineation thresholds of ANI (95%) and dDDH (70%)	1379:1452	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	7	10	theme	main	835:838	arg1	lipids					846:851	The main polar lipids	831:851	The main polar lipids	831:851	The main polar lipids included phosphatidylmethylethanolamine, phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol.
34762578	3	11	dep	optimum	405:411	arg1	NaCl					418:421	0 % NaCl	414:421	0 % NaCl	414:421	Growth was observed at 30-60 °C (optimum, 50 °C) and pH 6.0-9.0 (optimum, pH 7.0), with up to 2.0 % (w/v) NaCl (optimum, 0 % NaCl).
34762578	11	12	theme	physiological	1477:1489	arg1	properties					1491:1500	the observed physiological properties	1464:1500	the observed physiological properties	1464:1500	Based on the observed physiological properties, chemotaxonomic characteristics and ANI and dDDH values, FJAT-54423T and FJAT-54424 belong to a novel species of the genus Brevibacillus, for which the name Brevibacillus composti sp.
34762578	13	13	theme	1.2054T=KCTC	1743:1754	arg1	FJAT-54423T					1723:1733	FJAT-54423T	1723:1733	FJAT-54423T (=GDMCC 1.2054T=KCTC 43273T)	1723:1762	The type strain is FJAT-54423T (=GDMCC 1.2054T=KCTC 43273T).
34762578	13	13	theme	1.2054T=KCTC	1743:1754	arg1	43273T					1756:1761	=GDMCC 1.2054T=KCTC 43273T	1736:1761	=GDMCC 1.2054T=KCTC 43273T	1736:1761	The type strain is FJAT-54423T (=GDMCC 1.2054T=KCTC 43273T).
34762578	6	14	theme	fatty	781:785	arg1	iso-C15 					798:805	iso-C15 	798:805	iso-C15 	798:805	The menaquinone was MK-7, and the major fatty acids were iso-C15 : 0 and anteiso-C15 : 0.
34762578	6	14	theme	fatty	781:785	arg1	acids					787:791	the major fatty acids	771:791	the major fatty acids	771:791	The menaquinone was MK-7, and the major fatty acids were iso-C15 : 0 and anteiso-C15 : 0.
34762578	10	15	theme	DSM	1311:1313	arg1	6347T					1315:1319	B. borstelensis DSM 6347T	1295:1319	B. borstelensis DSM 6347T	1295:1319	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	11	16	theme	chemotaxonomic	1503:1516	arg1	characteristics					1518:1532	chemotaxonomic characteristics	1503:1532	chemotaxonomic characteristics	1503:1532	Based on the observed physiological properties, chemotaxonomic characteristics and ANI and dDDH values, FJAT-54423T and FJAT-54424 belong to a novel species of the genus Brevibacillus, for which the name Brevibacillus composti sp.
34762578	9	17	dep	strains	1067:1073	arg1	strains					1067:1073	strains FJAT-54423T and FJAT-54424	1067:1100	strains FJAT-54423T and FJAT-54424	1067:1100	The DNA G+C content of strains FJAT-54423T and FJAT-54424 were 54.3 and 54.4 mol%, respectively.
34762578	9	17	dep	strains	1067:1073	arg1	FJAT-54424					1091:1100	FJAT-54424	1091:1100	FJAT-54424	1091:1100	The DNA G+C content of strains FJAT-54423T and FJAT-54424 were 54.3 and 54.4 mol%, respectively.
34762578	9	17	dep	strains	1067:1073	arg1	FJAT-54423T					1075:1085	FJAT-54423T	1075:1085	FJAT-54423T	1075:1085	The DNA G+C content of strains FJAT-54423T and FJAT-54424 were 54.3 and 54.4 mol%, respectively.
34762578	6	18	dep	iso-C15 	798:805	arg1	anteiso-C15 					814:825	anteiso-C15 	814:825	anteiso-C15 	814:825	The menaquinone was MK-7, and the major fatty acids were iso-C15 : 0 and anteiso-C15 : 0.
34762578	6	18	dep	iso-C15 	798:805	arg1	 0					807:808	 0	807:808	 0	807:808	The menaquinone was MK-7, and the major fatty acids were iso-C15 : 0 and anteiso-C15 : 0.
34762578	6	18	dep	iso-C15 	798:805	arg1	 0					827:828	 0	827:828	 0	827:828	The menaquinone was MK-7, and the major fatty acids were iso-C15 : 0 and anteiso-C15 : 0.
34762578	3	19	dep	30-60 °C	316:323	arg1	50 °C					335:339	50 °C	335:339	50 °C	335:339	Growth was observed at 30-60 °C (optimum, 50 °C) and pH 6.0-9.0 (optimum, pH 7.0), with up to 2.0 % (w/v) NaCl (optimum, 0 % NaCl).
34762578	3	19	dep	30-60 °C	316:323	arg1	optimum					326:332	optimum	326:332	optimum	326:332	Growth was observed at 30-60 °C (optimum, 50 °C) and pH 6.0-9.0 (optimum, pH 7.0), with up to 2.0 % (w/v) NaCl (optimum, 0 % NaCl).
34762578	6	20	theme	major	775:779	arg1	iso-C15 					798:805	iso-C15 	798:805	iso-C15 	798:805	The menaquinone was MK-7, and the major fatty acids were iso-C15 : 0 and anteiso-C15 : 0.
34762578	6	20	theme	major	775:779	arg1	acids					787:791	the major fatty acids	771:791	the major fatty acids	771:791	The menaquinone was MK-7, and the major fatty acids were iso-C15 : 0 and anteiso-C15 : 0.
34762578	2	21	attach	isolated	215:222	arg2	strains					162:168	Two aerobic, Gram-stain-positive, rod-shaped, endospore-forming, thermophilic bacterial strains	74:168	Two aerobic, Gram-stain-positive, rod-shaped, endospore-forming, thermophilic bacterial strains	74:168	Two aerobic, Gram-stain-positive, rod-shaped, endospore-forming, thermophilic bacterial strains, designated FJAT-54423T and FJAT-54424, were isolated from hyperthermophilic compost sampled in Shanxi Province, PR China.
34762578	2	21	attach	isolated	215:222	arg1	compost					247:253	hyperthermophilic compost	229:253	hyperthermophilic compost sampled in Shanxi Province, PR China	229:290	Two aerobic, Gram-stain-positive, rod-shaped, endospore-forming, thermophilic bacterial strains, designated FJAT-54423T and FJAT-54424, were isolated from hyperthermophilic compost sampled in Shanxi Province, PR China.
34762578	10	22	dep	delineation	1403:1413	arg1	thresholds					1415:1424	thresholds	1415:1424	the recommended species delineation thresholds of ANI (95%) and dDDH (70%)	1379:1452	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	2	23	theme	bacterial	152:160	arg1	strains					162:168	Two aerobic, Gram-stain-positive, rod-shaped, endospore-forming, thermophilic bacterial strains	74:168	Two aerobic, Gram-stain-positive, rod-shaped, endospore-forming, thermophilic bacterial strains	74:168	Two aerobic, Gram-stain-positive, rod-shaped, endospore-forming, thermophilic bacterial strains, designated FJAT-54423T and FJAT-54424, were isolated from hyperthermophilic compost sampled in Shanxi Province, PR China.
34762578	8	24	theme	cell-wall	973:981	arg1	peptidoglycan					983:995	The cell-wall peptidoglycan	969:995	The cell-wall peptidoglycan	969:995	The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid.
34762578	10	25	dep	B.	1295:1296	arg1	borstelensis					1298:1309	borstelensis	1298:1309	borstelensis	1298:1309	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	5	26	theme	phylogenetic	619:630	arg1	trees					649:653	phylogenetic and phylogenomic trees	619:653	trees	649:653	Further, in phylogenetic and phylogenomic trees, strains FJAT-54423T and FJAT-54424 branched with members of the genus Brevibacillus.
34762578	0	27	theme	composti	14:21	arg1	sp					23:24	Brevibacillus composti sp	0:24	Brevibacillus composti sp.	0:25	Brevibacillus composti sp.
34762578	11	28	theme	dDDH	1546:1549	arg1	values					1551:1556	ANI and dDDH values	1538:1556	ANI and dDDH values	1538:1556	Based on the observed physiological properties, chemotaxonomic characteristics and ANI and dDDH values, FJAT-54423T and FJAT-54424 belong to a novel species of the genus Brevibacillus, for which the name Brevibacillus composti sp.
34762578	10	29	theme	21.5 	1335:1339	arg1	values					1220:1225	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values	1141:1225	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T	1141:1319	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	10	29	theme	21.5 	1335:1339	arg1	%					1340:1340	77.7 and 21.5 %	1326:1340	%	1340:1340	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	10	30	theme	hybridization	1199:1211	arg1	values					1220:1225	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values	1141:1225	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T	1141:1319	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	10	30	theme	hybridization	1199:1211	arg1	%					1340:1340	77.7 and 21.5 %	1326:1340	%	1340:1340	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	0	31	theme	Brevibacillus	0:12	arg1	sp					23:24	Brevibacillus composti sp	0:24	Brevibacillus composti sp.	0:25	Brevibacillus composti sp.
34762578	11	32	theme	composti	1673:1680	arg1	sp					1682:1683	the name Brevibacillus composti sp	1650:1683	the name Brevibacillus composti sp	1650:1683	Based on the observed physiological properties, chemotaxonomic characteristics and ANI and dDDH values, FJAT-54423T and FJAT-54424 belong to a novel species of the genus Brevibacillus, for which the name Brevibacillus composti sp.
34762578	10	33	theme	FJAT-54423T	1237:1247	arg1	values					1220:1225	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values	1141:1225	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T	1141:1319	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	10	33	theme	FJAT-54423T	1237:1247	arg1	%					1340:1340	77.7 and 21.5 %	1326:1340	%	1340:1340	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	13	34	theme	=GDMCC	1736:1741	arg1	FJAT-54423T					1723:1733	FJAT-54423T	1723:1733	FJAT-54423T (=GDMCC 1.2054T=KCTC 43273T)	1723:1762	The type strain is FJAT-54423T (=GDMCC 1.2054T=KCTC 43273T).
34762578	13	34	theme	=GDMCC	1736:1741	arg1	43273T					1756:1761	=GDMCC 1.2054T=KCTC 43273T	1736:1761	=GDMCC 1.2054T=KCTC 43273T	1736:1761	The type strain is FJAT-54423T (=GDMCC 1.2054T=KCTC 43273T).
34762578	10	35	dep	FJAT-54423T	1237:1247	arg1	6347T					1315:1319	B. borstelensis DSM 6347T	1295:1319	B. borstelensis DSM 6347T	1295:1319	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	10	36	theme	77.7	1326:1329	arg1	values					1220:1225	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values	1141:1225	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T	1141:1319	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	10	36	theme	77.7	1326:1329	arg1	%					1340:1340	77.7 and 21.5 %	1326:1340	%	1340:1340	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	10	37	theme	strain	1230:1235	arg1	FJAT-54423T					1237:1247	strain FJAT-54423T	1230:1247	strain FJAT-54423T	1230:1247	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	5	38	theme	genus	720:724	arg1	members					705:711	members	705:711	members of the genus Brevibacillus	705:738	Further, in phylogenetic and phylogenomic trees, strains FJAT-54423T and FJAT-54424 branched with members of the genus Brevibacillus.
34762578	11	39	theme	name	1654:1657	arg1	sp					1682:1683	the name Brevibacillus composti sp	1650:1683	the name Brevibacillus composti sp	1650:1683	Based on the observed physiological properties, chemotaxonomic characteristics and ANI and dDDH values, FJAT-54423T and FJAT-54424 belong to a novel species of the genus Brevibacillus, for which the name Brevibacillus composti sp.
34762578	5	40	dep	strains	656:662	arg1	FJAT-54424					680:689	FJAT-54424	680:689	FJAT-54424	680:689	Further, in phylogenetic and phylogenomic trees, strains FJAT-54423T and FJAT-54424 branched with members of the genus Brevibacillus.
34762578	5	40	dep	strains	656:662	arg1	FJAT-54423T					664:674	FJAT-54423T	664:674	FJAT-54423T	664:674	Further, in phylogenetic and phylogenomic trees, strains FJAT-54423T and FJAT-54424 branched with members of the genus Brevibacillus.
34762578	5	40	dep	strains	656:662	arg1	strains					656:662	strains	656:662	strains FJAT-54423T and FJAT-54424	656:689	Further, in phylogenetic and phylogenomic trees, strains FJAT-54423T and FJAT-54424 branched with members of the genus Brevibacillus.
34762578	9	41	theme	DNA	1048:1050	arg1	%					1124:1124	54.3 and 54.4 mol%	1107:1124	54.3 and 54.4 mol%	1107:1124	The DNA G+C content of strains FJAT-54423T and FJAT-54424 were 54.3 and 54.4 mol%, respectively.
34762578	9	41	theme	DNA	1048:1050	arg1	content					1056:1062	The DNA G+C content	1044:1062	The DNA G+C content of strains FJAT-54423T and FJAT-54424	1044:1100	The DNA G+C content of strains FJAT-54423T and FJAT-54424 were 54.3 and 54.4 mol%, respectively.
34762578	5	42	theme	Brevibacillus	726:738	arg1	genus					720:724	the genus Brevibacillus	716:738	the genus Brevibacillus	716:738	Further, in phylogenetic and phylogenomic trees, strains FJAT-54423T and FJAT-54424 branched with members of the genus Brevibacillus.
34762578	3	43	located	observed	304:311	arg1	pH					346:347	pH 6.0-9.0	346:355	pH 6.0-9.0 (optimum, pH 7.0)	346:373	Growth was observed at 30-60 °C (optimum, 50 °C) and pH 6.0-9.0 (optimum, pH 7.0), with up to 2.0 % (w/v) NaCl (optimum, 0 % NaCl).
34762578	3	43	located	observed	304:311	arg1	optimum					358:364	optimum	358:364	optimum	358:364	Growth was observed at 30-60 °C (optimum, 50 °C) and pH 6.0-9.0 (optimum, pH 7.0), with up to 2.0 % (w/v) NaCl (optimum, 0 % NaCl).
34762578	3	43	located	observed	304:311	arg1	30-60 °C					316:323	30-60 °C	316:323	30-60 °C (optimum, 50 °C)	316:340	Growth was observed at 30-60 °C (optimum, 50 °C) and pH 6.0-9.0 (optimum, pH 7.0), with up to 2.0 % (w/v) NaCl (optimum, 0 % NaCl).
34762578	3	43	located	observed	304:311	arg2	Growth					293:298	Growth	293:298	Growth	293:298	Growth was observed at 30-60 °C (optimum, 50 °C) and pH 6.0-9.0 (optimum, pH 7.0), with up to 2.0 % (w/v) NaCl (optimum, 0 % NaCl).
34762578	10	44	theme	species	1395:1401	arg1	delineation					1403:1413	the recommended species delineation thresholds	1379:1424	the recommended species delineation thresholds of ANI (95%) and dDDH (70%)	1379:1452	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	9	45	theme	G+C	1052:1054	arg1	%					1124:1124	54.3 and 54.4 mol%	1107:1124	54.3 and 54.4 mol%	1107:1124	The DNA G+C content of strains FJAT-54423T and FJAT-54424 were 54.3 and 54.4 mol%, respectively.
34762578	9	45	theme	G+C	1052:1054	arg1	content					1056:1062	The DNA G+C content	1044:1062	The DNA G+C content of strains FJAT-54423T and FJAT-54424	1044:1100	The DNA G+C content of strains FJAT-54423T and FJAT-54424 were 54.3 and 54.4 mol%, respectively.
34762578	4	46	theme	valid	541:545	arg1	taxon					547:551	a valid taxon	539:551	a valid taxon	539:551	The 16S rRNA gene sequence similarity between FJAT-54423T and FJAT-54424 was 99.9%, and the maximum similarity to a valid taxon was observed with Brevibacillus borstelensis (98.3%).
34762578	4	47	theme	rRNA	433:436	arg1	%					506:506	99.9%	502:506	99.9%	502:506	The 16S rRNA gene sequence similarity between FJAT-54423T and FJAT-54424 was 99.9%, and the maximum similarity to a valid taxon was observed with Brevibacillus borstelensis (98.3%).
34762578	4	47	theme	rRNA	433:436	arg1	similarity					452:461	The 16S rRNA gene sequence similarity	425:461	The 16S rRNA gene sequence similarity between FJAT-54423T and FJAT-54424	425:496	The 16S rRNA gene sequence similarity between FJAT-54423T and FJAT-54424 was 99.9%, and the maximum similarity to a valid taxon was observed with Brevibacillus borstelensis (98.3%).
34762578	8	48	theme	meso-diaminopimelic	1018:1036	arg1	acid					1038:1041	meso-diaminopimelic acid	1018:1041	meso-diaminopimelic acid	1018:1041	The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid.
34762578	3	49	dep	2.0 	387:390	arg1	to					384:385	to	384:385	to	384:385	Growth was observed at 30-60 °C (optimum, 50 °C) and pH 6.0-9.0 (optimum, pH 7.0), with up to 2.0 % (w/v) NaCl (optimum, 0 % NaCl).
34762578	4	50	theme	gene	438:441	arg1	%					506:506	99.9%	502:506	99.9%	502:506	The 16S rRNA gene sequence similarity between FJAT-54423T and FJAT-54424 was 99.9%, and the maximum similarity to a valid taxon was observed with Brevibacillus borstelensis (98.3%).
34762578	4	50	theme	gene	438:441	arg1	similarity					452:461	The 16S rRNA gene sequence similarity	425:461	The 16S rRNA gene sequence similarity between FJAT-54423T and FJAT-54424	425:496	The 16S rRNA gene sequence similarity between FJAT-54423T and FJAT-54424 was 99.9%, and the maximum similarity to a valid taxon was observed with Brevibacillus borstelensis (98.3%).
34762578	4	51	theme	16S	429:431	arg1	%					506:506	99.9%	502:506	99.9%	502:506	The 16S rRNA gene sequence similarity between FJAT-54423T and FJAT-54424 was 99.9%, and the maximum similarity to a valid taxon was observed with Brevibacillus borstelensis (98.3%).
34762578	4	51	theme	16S	429:431	arg1	similarity					452:461	The 16S rRNA gene sequence similarity	425:461	The 16S rRNA gene sequence similarity between FJAT-54423T and FJAT-54424	425:496	The 16S rRNA gene sequence similarity between FJAT-54423T and FJAT-54424 was 99.9%, and the maximum similarity to a valid taxon was observed with Brevibacillus borstelensis (98.3%).
34762578	3	52	dep	%	391:391	arg1	2.0 					387:390	2.0 	387:390	2.0 	387:390	Growth was observed at 30-60 °C (optimum, 50 °C) and pH 6.0-9.0 (optimum, pH 7.0), with up to 2.0 % (w/v) NaCl (optimum, 0 % NaCl).
34762578	3	52	dep	%	391:391	arg1	w/v					394:396	w/v	394:396	w/v	394:396	Growth was observed at 30-60 °C (optimum, 50 °C) and pH 6.0-9.0 (optimum, pH 7.0), with up to 2.0 % (w/v) NaCl (optimum, 0 % NaCl).
34762578	10	53	theme	recommended	1383:1393	arg1	delineation					1403:1413	the recommended species delineation thresholds	1379:1424	the recommended species delineation thresholds of ANI (95%) and dDDH (70%)	1379:1452	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	4	54	theme	sequence	443:450	arg1	%					506:506	99.9%	502:506	99.9%	502:506	The 16S rRNA gene sequence similarity between FJAT-54423T and FJAT-54424 was 99.9%, and the maximum similarity to a valid taxon was observed with Brevibacillus borstelensis (98.3%).
34762578	4	54	theme	sequence	443:450	arg1	similarity					452:461	The 16S rRNA gene sequence similarity	425:461	The 16S rRNA gene sequence similarity between FJAT-54423T and FJAT-54424	425:496	The 16S rRNA gene sequence similarity between FJAT-54423T and FJAT-54424 was 99.9%, and the maximum similarity to a valid taxon was observed with Brevibacillus borstelensis (98.3%).
34762578	11	55	theme	novel	1598:1602	arg1	species					1604:1610	a novel species	1596:1610	a novel species of the genus Brevibacillus, for which the name Brevibacillus composti sp	1596:1683	Based on the observed physiological properties, chemotaxonomic characteristics and ANI and dDDH values, FJAT-54423T and FJAT-54424 belong to a novel species of the genus Brevibacillus, for which the name Brevibacillus composti sp.
34762578	2	56	theme	hyperthermophilic	229:245	arg1	compost					247:253	hyperthermophilic compost	229:253	hyperthermophilic compost sampled in Shanxi Province, PR China	229:290	Two aerobic, Gram-stain-positive, rod-shaped, endospore-forming, thermophilic bacterial strains, designated FJAT-54423T and FJAT-54424, were isolated from hyperthermophilic compost sampled in Shanxi Province, PR China.
34762578	9	57	theme	strains	1067:1073	arg1	%					1124:1124	54.3 and 54.4 mol%	1107:1124	54.3 and 54.4 mol%	1107:1124	The DNA G+C content of strains FJAT-54423T and FJAT-54424 were 54.3 and 54.4 mol%, respectively.
34762578	9	57	theme	strains	1067:1073	arg1	content					1056:1062	The DNA G+C content	1044:1062	The DNA G+C content of strains FJAT-54423T and FJAT-54424	1044:1100	The DNA G+C content of strains FJAT-54423T and FJAT-54424 were 54.3 and 54.4 mol%, respectively.
34762578	3	58	theme	%	416:416	arg1	NaCl					418:421	0 % NaCl	414:421	0 % NaCl	414:421	Growth was observed at 30-60 °C (optimum, 50 °C) and pH 6.0-9.0 (optimum, pH 7.0), with up to 2.0 % (w/v) NaCl (optimum, 0 % NaCl).
34762578	2	59	theme	aerobic	78:84	arg1	strains					162:168	Two aerobic, Gram-stain-positive, rod-shaped, endospore-forming, thermophilic bacterial strains	74:168	Two aerobic, Gram-stain-positive, rod-shaped, endospore-forming, thermophilic bacterial strains	74:168	Two aerobic, Gram-stain-positive, rod-shaped, endospore-forming, thermophilic bacterial strains, designated FJAT-54423T and FJAT-54424, were isolated from hyperthermophilic compost sampled in Shanxi Province, PR China.
34762578	10	60	theme	ANI	1429:1431	arg1	delineation					1403:1413	the recommended species delineation thresholds	1379:1424	the recommended species delineation thresholds of ANI (95%) and dDDH (70%)	1379:1452	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	11	61	theme	Brevibacillus	1659:1671	arg1	sp					1682:1683	the name Brevibacillus composti sp	1650:1683	the name Brevibacillus composti sp	1650:1683	Based on the observed physiological properties, chemotaxonomic characteristics and ANI and dDDH values, FJAT-54423T and FJAT-54424 belong to a novel species of the genus Brevibacillus, for which the name Brevibacillus composti sp.
34762578	2	62	theme	PR	283:284	arg1	Province					273:280	Shanxi Province	266:280	Shanxi Province	266:280	Two aerobic, Gram-stain-positive, rod-shaped, endospore-forming, thermophilic bacterial strains, designated FJAT-54423T and FJAT-54424, were isolated from hyperthermophilic compost sampled in Shanxi Province, PR China.
34762578	2	62	theme	PR	283:284	arg1	China					286:290	PR China	283:290	PR China	283:290	Two aerobic, Gram-stain-positive, rod-shaped, endospore-forming, thermophilic bacterial strains, designated FJAT-54423T and FJAT-54424, were isolated from hyperthermophilic compost sampled in Shanxi Province, PR China.
34762578	10	63	theme	reference	1278:1286	arg1	strain					1288:1293	its most closely related reference strain	1253:1293	its most closely related reference strain	1253:1293	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	11	64	theme	genus	1619:1623	arg1	species					1604:1610	a novel species	1596:1610	a novel species of the genus Brevibacillus, for which the name Brevibacillus composti sp	1596:1683	Based on the observed physiological properties, chemotaxonomic characteristics and ANI and dDDH values, FJAT-54423T and FJAT-54424 belong to a novel species of the genus Brevibacillus, for which the name Brevibacillus composti sp.
34762578	1	65	theme	hyperthermophilic	47:63	arg1	compost					65:71	hyperthermophilic compost	47:71	hyperthermophilic compost	47:71	nov., isolated from hyperthermophilic compost.
34762578	10	66	theme	B.	1295:1296	arg1	6347T					1315:1319	B. borstelensis DSM 6347T	1295:1319	B. borstelensis DSM 6347T	1295:1319	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	10	67	theme	identity	1164:1171	arg1	values					1220:1225	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values	1141:1225	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T	1141:1319	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	10	67	theme	identity	1164:1171	arg1	%					1340:1340	77.7 and 21.5 %	1326:1340	%	1340:1340	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	11	68	theme	Brevibacillus	1625:1637	arg1	genus					1619:1623	the genus Brevibacillus	1615:1637	the genus Brevibacillus	1615:1637	Based on the observed physiological properties, chemotaxonomic characteristics and ANI and dDDH values, FJAT-54423T and FJAT-54424 belong to a novel species of the genus Brevibacillus, for which the name Brevibacillus composti sp.
34762578	5	69	theme	phylogenomic	636:647	arg1	trees					649:653	phylogenetic and phylogenomic trees	619:653	trees	649:653	Further, in phylogenetic and phylogenomic trees, strains FJAT-54423T and FJAT-54424 branched with members of the genus Brevibacillus.
34762578	3	70	dep	NaCl	399:402	arg1	%					391:391	%	391:391	%	391:391	Growth was observed at 30-60 °C (optimum, 50 °C) and pH 6.0-9.0 (optimum, pH 7.0), with up to 2.0 % (w/v) NaCl (optimum, 0 % NaCl).
34762578	10	71	theme	strain	1288:1293	arg1	values					1220:1225	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values	1141:1225	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T	1141:1319	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	10	71	theme	strain	1288:1293	arg1	%					1340:1340	77.7 and 21.5 %	1326:1340	%	1340:1340	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	10	72	theme	nucleotide	1153:1162	arg1	ANI					1174:1176	ANI	1174:1176	ANI	1174:1176	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	10	72	theme	nucleotide	1153:1162	arg1	identity					1164:1171	average nucleotide identity	1145:1171	average nucleotide identity (ANI)	1145:1177	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	4	73	theme	maximum	517:523	arg1	similarity					525:534	the maximum similarity	513:534	the maximum similarity to a valid taxon	513:551	The 16S rRNA gene sequence similarity between FJAT-54423T and FJAT-54424 was 99.9%, and the maximum similarity to a valid taxon was observed with Brevibacillus borstelensis (98.3%).
34762578	2	74	theme	Shanxi	266:271	arg1	Province					273:280	Shanxi Province	266:280	Shanxi Province	266:280	Two aerobic, Gram-stain-positive, rod-shaped, endospore-forming, thermophilic bacterial strains, designated FJAT-54423T and FJAT-54424, were isolated from hyperthermophilic compost sampled in Shanxi Province, PR China.
34762578	2	74	theme	Shanxi	266:271	arg1	China					286:290	PR China	283:290	PR China	283:290	Two aerobic, Gram-stain-positive, rod-shaped, endospore-forming, thermophilic bacterial strains, designated FJAT-54423T and FJAT-54424, were isolated from hyperthermophilic compost sampled in Shanxi Province, PR China.
34762578	7	75	theme	polar	840:844	arg1	lipids					846:851	The main polar lipids	831:851	The main polar lipids	831:851	The main polar lipids included phosphatidylmethylethanolamine, phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol.
34762578	4	76	theme	Brevibacillus	571:583	arg1	borstelensis					585:596	Brevibacillus borstelensis	571:596	Brevibacillus borstelensis (98.3%)	571:604	The 16S rRNA gene sequence similarity between FJAT-54423T and FJAT-54424 was 99.9%, and the maximum similarity to a valid taxon was observed with Brevibacillus borstelensis (98.3%).
34762578	4	76	theme	Brevibacillus	571:583	arg1	%					603:603	98.3%	599:603	98.3%	599:603	The 16S rRNA gene sequence similarity between FJAT-54423T and FJAT-54424 was 99.9%, and the maximum similarity to a valid taxon was observed with Brevibacillus borstelensis (98.3%).
34762578	10	77	theme	DNA-DNA	1191:1197	arg1	dDDH					1214:1217	dDDH	1214:1217	dDDH	1214:1217	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
34762578	10	77	theme	DNA-DNA	1191:1197	arg1	hybridization					1199:1211	digital DNA-DNA hybridization	1183:1211	digital DNA-DNA hybridization (dDDH)	1183:1218	The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%).
33620525	7	0	theme	amino	1061:1065	arg1	acids					1067:1071	The amino acids	1057:1071	The amino acids in peptidoglycan of strain DCY120T	1057:1106	The amino acids in peptidoglycan of strain DCY120T were lysine, alanine, glutamic acid, and aspartic acid.
33620525	7	0	theme	amino	1061:1065	arg1	lysine					1113:1118	lysine	1113:1118	lysine	1113:1118	The amino acids in peptidoglycan of strain DCY120T were lysine, alanine, glutamic acid, and aspartic acid.
33620525	2	1	theme	novel	86:90	arg1	DCY120T					203:209	DCY120T	203:209	DCY120T	203:209	A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain, DCY120T, was isolated from the gut of honeybee (Apis cerana) in Gyeonggi-do, South Korea.
33620525	2	1	theme	novel	86:90	arg1	strain					195:200	A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain	84:200	A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain	84:200	A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain, DCY120T, was isolated from the gut of honeybee (Apis cerana) in Gyeonggi-do, South Korea.
33620525	4	2	theme	species	764:770	arg1	delineation					772:782	species delineation	764:782	species delineation	764:782	The genome of strain DCY120T was sequenced and the average nucleotide identity (ANI) between strain DCY120T and the related Bombilactobacillus type strains were below the threshold value (95-96%) for species delineation.
33620525	5	3	theme	fatty	795:799	arg1	C16:0					812:816	C16:0	812:816	C16:0	812:816	The major fatty acids were C16:0, C18:1 ω9c, Summed C19:1 ω6c/C19:0 cyclo ω10c/C19:0 ω6 and Summed C18:1 ω7c/C18:1 ω6c.
33620525	5	3	theme	fatty	795:799	arg1	acids					801:805	The major fatty acids	785:805	The major fatty acids	785:805	The major fatty acids were C16:0, C18:1 ω9c, Summed C19:1 ω6c/C19:0 cyclo ω10c/C19:0 ω6 and Summed C18:1 ω7c/C18:1 ω6c.
33620525	8	4	theme	name	1327:1330	arg1	sp					1357:1358	the name Bombilactobacillus apium sp	1323:1358	the name Bombilactobacillus apium sp	1323:1358	In conclusion, the description of phenotypic and genotypic properties support strain DCY120T as a novel species within the genus Bombilactobacillus, for which the name Bombilactobacillus apium sp.
33620525	8	5	theme	novel	1262:1266	arg1	DCY120T					1249:1255	strain DCY120T	1242:1255	strain DCY120T	1242:1255	In conclusion, the description of phenotypic and genotypic properties support strain DCY120T as a novel species within the genus Bombilactobacillus, for which the name Bombilactobacillus apium sp.
33620525	8	5	theme	novel	1262:1266	arg1	species					1268:1274	a novel species	1260:1274	a novel species	1260:1274	In conclusion, the description of phenotypic and genotypic properties support strain DCY120T as a novel species within the genus Bombilactobacillus, for which the name Bombilactobacillus apium sp.
33620525	7	6	theme	strain	1093:1098	arg1	DCY120T					1100:1106	strain DCY120T	1093:1106	strain DCY120T	1093:1106	The amino acids in peptidoglycan of strain DCY120T were lysine, alanine, glutamic acid, and aspartic acid.
33620525	10	7	theme	= KCTC	1407:1412	arg1	DCY120T					1398:1404	DCY120T	1398:1404	DCY120T (= KCTC 43194T = JCM 34006T)	1398:1433	The type strain is DCY120T (= KCTC 43194T = JCM 34006T).
33620525	10	7	theme	= KCTC	1407:1412	arg1	34006T					1427:1432	= KCTC 43194T = JCM 34006T	1407:1432	= KCTC 43194T = JCM 34006T	1407:1432	The type strain is DCY120T (= KCTC 43194T = JCM 34006T).
33620525	4	8	theme	Bombilactobacillus	688:705	arg1	strains					712:718	the related Bombilactobacillus type strains	676:718	the related Bombilactobacillus type strains	676:718	The genome of strain DCY120T was sequenced and the average nucleotide identity (ANI) between strain DCY120T and the related Bombilactobacillus type strains were below the threshold value (95-96%) for species delineation.
33620525	5	9	theme	ω6c/C19:0	843:851	arg1	ω6					870:871	Summed C19:1 ω6c/C19:0 cyclo ω10c/C19:0 ω6	830:871	Summed C19:1 ω6c/C19:0 cyclo ω10c/C19:0 ω6	830:871	The major fatty acids were C16:0, C18:1 ω9c, Summed C19:1 ω6c/C19:0 cyclo ω10c/C19:0 ω6 and Summed C18:1 ω7c/C18:1 ω6c.
33620525	5	10	theme	C19:1	837:841	arg1	ω6					870:871	Summed C19:1 ω6c/C19:0 cyclo ω10c/C19:0 ω6	830:871	Summed C19:1 ω6c/C19:0 cyclo ω10c/C19:0 ω6	830:871	The major fatty acids were C16:0, C18:1 ω9c, Summed C19:1 ω6c/C19:0 cyclo ω10c/C19:0 ω6 and Summed C18:1 ω7c/C18:1 ω6c.
33620525	8	11	dep	Bombilactobacillus	1332:1349	arg1	apium					1351:1355	apium	1351:1355	apium	1351:1355	In conclusion, the description of phenotypic and genotypic properties support strain DCY120T as a novel species within the genus Bombilactobacillus, for which the name Bombilactobacillus apium sp.
33620525	5	12	theme	cyclo	853:857	arg1	ω6					870:871	Summed C19:1 ω6c/C19:0 cyclo ω10c/C19:0 ω6	830:871	Summed C19:1 ω6c/C19:0 cyclo ω10c/C19:0 ω6	830:871	The major fatty acids were C16:0, C18:1 ω9c, Summed C19:1 ω6c/C19:0 cyclo ω10c/C19:0 ω6 and Summed C18:1 ω7c/C18:1 ω6c.
33620525	2	13	theme	honeybee	241:248	arg1	gut					234:236	the gut	230:236	the gut of honeybee (Apis cerana) in Gyeonggi-do, South Korea	230:290	A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain, DCY120T, was isolated from the gut of honeybee (Apis cerana) in Gyeonggi-do, South Korea.
33620525	4	14	theme	related	680:686	arg1	strains					712:718	the related Bombilactobacillus type strains	676:718	the related Bombilactobacillus type strains	676:718	The genome of strain DCY120T was sequenced and the average nucleotide identity (ANI) between strain DCY120T and the related Bombilactobacillus type strains were below the threshold value (95-96%) for species delineation.
33620525	7	15	theme	aspartic	1149:1156	arg1	acid					1158:1161	aspartic acid	1149:1161	aspartic acid	1149:1161	The amino acids in peptidoglycan of strain DCY120T were lysine, alanine, glutamic acid, and aspartic acid.
33620525	3	16	theme	Strain	293:298	arg1	DCY120T					300:306	Strain DCY120T	293:306	Strain DCY120T	293:306	Strain DCY120T belongs to the genus Bombilactobacillus and is moderately related to Bombilactobacillus mellis Hon2T (94.1% similarity), Bombilactobacillus bombi BTLCH M1/2T (93.8%), and Bombilactobacillus mellifer Bin4NT (93.5%) based on 16S rRNA gene sequence analysis.
33620525	1	17	theme	honeybee	60:67	arg1	gut					53:55	the gut	49:55	the gut of honeybee (Apis cerana)	49:81	nov., isolated from the gut of honeybee (Apis cerana).
33620525	3	18	theme	genus	323:327	arg1	Bombilactobacillus					329:346	the genus Bombilactobacillus	319:346	the genus Bombilactobacillus	319:346	Strain DCY120T belongs to the genus Bombilactobacillus and is moderately related to Bombilactobacillus mellis Hon2T (94.1% similarity), Bombilactobacillus bombi BTLCH M1/2T (93.8%), and Bombilactobacillus mellifer Bin4NT (93.5%) based on 16S rRNA gene sequence analysis.
33620525	2	19	theme	positive-	106:114	arg1	DCY120T					203:209	DCY120T	203:209	DCY120T	203:209	A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain, DCY120T, was isolated from the gut of honeybee (Apis cerana) in Gyeonggi-do, South Korea.
33620525	2	19	theme	positive-	106:114	arg1	strain					195:200	A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain	84:200	A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain	84:200	A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain, DCY120T, was isolated from the gut of honeybee (Apis cerana) in Gyeonggi-do, South Korea.
33620525	8	20	theme	phenotypic	1198:1207	arg1	properties					1223:1232	phenotypic and genotypic properties	1198:1232	phenotypic and genotypic properties	1198:1232	In conclusion, the description of phenotypic and genotypic properties support strain DCY120T as a novel species within the genus Bombilactobacillus, for which the name Bombilactobacillus apium sp.
33620525	4	21	theme	DCY120T	585:591	arg1	genome					568:573	The genome	564:573	The genome of strain DCY120T	564:591	The genome of strain DCY120T was sequenced and the average nucleotide identity (ANI) between strain DCY120T and the related Bombilactobacillus type strains were below the threshold value (95-96%) for species delineation.
33620525	0	22	theme	Bombilactobacillus	0:17	arg1	sp					25:26	Bombilactobacillus apium sp	0:26	Bombilactobacillus apium sp.	0:27	Bombilactobacillus apium sp.
33620525	1	23	theme	Apis	70:73	arg1	honeybee					60:67	honeybee	60:67	honeybee (Apis cerana)	60:81	nov., isolated from the gut of honeybee (Apis cerana).
33620525	1	23	theme	Apis	70:73	arg1	cerana					75:80	Apis cerana	70:80	Apis cerana	70:80	nov., isolated from the gut of honeybee (Apis cerana).
33620525	4	24	theme	strain	578:583	arg1	DCY120T					585:591	strain DCY120T	578:591	strain DCY120T	578:591	The genome of strain DCY120T was sequenced and the average nucleotide identity (ANI) between strain DCY120T and the related Bombilactobacillus type strains were below the threshold value (95-96%) for species delineation.
33620525	2	25	dep	positive-	106:114	arg1	rod-shaped					149:158	rod-shaped	149:158	rod-shaped	149:158	A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain, DCY120T, was isolated from the gut of honeybee (Apis cerana) in Gyeonggi-do, South Korea.
33620525	2	25	dep	positive-	106:114	arg1	anaerobic					175:183	anaerobic	175:183	anaerobic	175:183	A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain, DCY120T, was isolated from the gut of honeybee (Apis cerana) in Gyeonggi-do, South Korea.
33620525	2	25	dep	positive-	106:114	arg1	negative-					138:146	negative-	138:146	negative-	138:146	A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain, DCY120T, was isolated from the gut of honeybee (Apis cerana) in Gyeonggi-do, South Korea.
33620525	8	26	theme	strain	1242:1247	arg1	DCY120T					1249:1255	strain DCY120T	1242:1255	strain DCY120T	1242:1255	In conclusion, the description of phenotypic and genotypic properties support strain DCY120T as a novel species within the genus Bombilactobacillus, for which the name Bombilactobacillus apium sp.
33620525	8	26	theme	strain	1242:1247	arg1	species					1268:1274	a novel species	1260:1274	a novel species	1260:1274	In conclusion, the description of phenotypic and genotypic properties support strain DCY120T as a novel species within the genus Bombilactobacillus, for which the name Bombilactobacillus apium sp.
33620525	4	27	theme	strain	657:662	arg1	DCY120T					664:670	strain DCY120T	657:670	strain DCY120T	657:670	The genome of strain DCY120T was sequenced and the average nucleotide identity (ANI) between strain DCY120T and the related Bombilactobacillus type strains were below the threshold value (95-96%) for species delineation.
33620525	3	28	theme	%	414:414	arg1	Hon2T					403:407	Hon2T	403:407	Hon2T	403:407	Strain DCY120T belongs to the genus Bombilactobacillus and is moderately related to Bombilactobacillus mellis Hon2T (94.1% similarity), Bombilactobacillus bombi BTLCH M1/2T (93.8%), and Bombilactobacillus mellifer Bin4NT (93.5%) based on 16S rRNA gene sequence analysis.
33620525	3	28	theme	%	414:414	arg1	similarity					416:425	94.1% similarity	410:425	94.1% similarity	410:425	Strain DCY120T belongs to the genus Bombilactobacillus and is moderately related to Bombilactobacillus mellis Hon2T (94.1% similarity), Bombilactobacillus bombi BTLCH M1/2T (93.8%), and Bombilactobacillus mellifer Bin4NT (93.5%) based on 16S rRNA gene sequence analysis.
33620525	10	29	theme	43194T = JCM	1414:1425	arg1	DCY120T					1398:1404	DCY120T	1398:1404	DCY120T (= KCTC 43194T = JCM 34006T)	1398:1433	The type strain is DCY120T (= KCTC 43194T = JCM 34006T).
33620525	10	29	theme	43194T = JCM	1414:1425	arg1	34006T					1427:1432	= KCTC 43194T = JCM 34006T	1407:1432	= KCTC 43194T = JCM 34006T	1407:1432	The type strain is DCY120T (= KCTC 43194T = JCM 34006T).
33620525	5	30	theme	ω10c/C19:0	859:868	arg1	ω6					870:871	Summed C19:1 ω6c/C19:0 cyclo ω10c/C19:0 ω6	830:871	Summed C19:1 ω6c/C19:0 cyclo ω10c/C19:0 ω6	830:871	The major fatty acids were C16:0, C18:1 ω9c, Summed C19:1 ω6c/C19:0 cyclo ω10c/C19:0 ω6 and Summed C18:1 ω7c/C18:1 ω6c.
33620525	2	31	theme	Apis	251:254	arg1	honeybee					241:248	honeybee	241:248	honeybee (Apis cerana)	241:262	A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain, DCY120T, was isolated from the gut of honeybee (Apis cerana) in Gyeonggi-do, South Korea.
33620525	2	31	theme	Apis	251:254	arg1	cerana					256:261	Apis cerana	251:261	Apis cerana	251:261	A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain, DCY120T, was isolated from the gut of honeybee (Apis cerana) in Gyeonggi-do, South Korea.
33620525	7	32	theme	glutamic	1130:1137	arg1	acid					1139:1142	glutamic acid	1130:1142	glutamic acid	1130:1142	The amino acids in peptidoglycan of strain DCY120T were lysine, alanine, glutamic acid, and aspartic acid.
33620525	3	33	theme	sequence	545:552	arg1	analysis					554:561	16S rRNA gene sequence analysis	531:561	16S rRNA gene sequence analysis	531:561	Strain DCY120T belongs to the genus Bombilactobacillus and is moderately related to Bombilactobacillus mellis Hon2T (94.1% similarity), Bombilactobacillus bombi BTLCH M1/2T (93.8%), and Bombilactobacillus mellifer Bin4NT (93.5%) based on 16S rRNA gene sequence analysis.
33620525	8	34	theme	properties	1223:1232	arg1	description					1183:1193	the description	1179:1193	the description of phenotypic and genotypic properties	1179:1232	In conclusion, the description of phenotypic and genotypic properties support strain DCY120T as a novel species within the genus Bombilactobacillus, for which the name Bombilactobacillus apium sp.
33620525	3	35	theme	94.1	410:413	arg1	%					414:414	%	414:414	%	414:414	Strain DCY120T belongs to the genus Bombilactobacillus and is moderately related to Bombilactobacillus mellis Hon2T (94.1% similarity), Bombilactobacillus bombi BTLCH M1/2T (93.8%), and Bombilactobacillus mellifer Bin4NT (93.5%) based on 16S rRNA gene sequence analysis.
33620525	8	36	theme	genus	1287:1291	arg1	Bombilactobacillus					1293:1310	the genus Bombilactobacillus	1283:1310	the genus Bombilactobacillus	1283:1310	In conclusion, the description of phenotypic and genotypic properties support strain DCY120T as a novel species within the genus Bombilactobacillus, for which the name Bombilactobacillus apium sp.
33620525	3	37	dep	mellis	396:401	arg1	M1/2T					460:464	M1/2T	460:464	M1/2T	460:464	Strain DCY120T belongs to the genus Bombilactobacillus and is moderately related to Bombilactobacillus mellis Hon2T (94.1% similarity), Bombilactobacillus bombi BTLCH M1/2T (93.8%), and Bombilactobacillus mellifer Bin4NT (93.5%) based on 16S rRNA gene sequence analysis.
33620525	3	37	dep	mellis	396:401	arg1	%					471:471	93.8%	467:471	93.8%	467:471	Strain DCY120T belongs to the genus Bombilactobacillus and is moderately related to Bombilactobacillus mellis Hon2T (94.1% similarity), Bombilactobacillus bombi BTLCH M1/2T (93.8%), and Bombilactobacillus mellifer Bin4NT (93.5%) based on 16S rRNA gene sequence analysis.
33620525	3	37	dep	mellis	396:401	arg1	Hon2T					403:407	Hon2T	403:407	Hon2T	403:407	Strain DCY120T belongs to the genus Bombilactobacillus and is moderately related to Bombilactobacillus mellis Hon2T (94.1% similarity), Bombilactobacillus bombi BTLCH M1/2T (93.8%), and Bombilactobacillus mellifer Bin4NT (93.5%) based on 16S rRNA gene sequence analysis.
33620525	3	37	dep	mellis	396:401	arg1	Bin4NT					507:512	Bin4NT	507:512	Bin4NT	507:512	Strain DCY120T belongs to the genus Bombilactobacillus and is moderately related to Bombilactobacillus mellis Hon2T (94.1% similarity), Bombilactobacillus bombi BTLCH M1/2T (93.8%), and Bombilactobacillus mellifer Bin4NT (93.5%) based on 16S rRNA gene sequence analysis.
33620525	3	37	dep	mellis	396:401	arg1	similarity					416:425	94.1% similarity	410:425	94.1% similarity	410:425	Strain DCY120T belongs to the genus Bombilactobacillus and is moderately related to Bombilactobacillus mellis Hon2T (94.1% similarity), Bombilactobacillus bombi BTLCH M1/2T (93.8%), and Bombilactobacillus mellifer Bin4NT (93.5%) based on 16S rRNA gene sequence analysis.
33620525	4	38	theme	nucleotide	623:632	arg1	ANI					644:646	ANI	644:646	ANI	644:646	The genome of strain DCY120T was sequenced and the average nucleotide identity (ANI) between strain DCY120T and the related Bombilactobacillus type strains were below the threshold value (95-96%) for species delineation.
33620525	4	38	theme	nucleotide	623:632	arg1	identity					634:641	the average nucleotide identity	611:641	the average nucleotide identity (ANI) between strain DCY120T and the related Bombilactobacillus type strains	611:718	The genome of strain DCY120T was sequenced and the average nucleotide identity (ANI) between strain DCY120T and the related Bombilactobacillus type strains were below the threshold value (95-96%) for species delineation.
33620525	5	39	theme	Summed	830:835	arg1	ω6					870:871	Summed C19:1 ω6c/C19:0 cyclo ω10c/C19:0 ω6	830:871	Summed C19:1 ω6c/C19:0 cyclo ω10c/C19:0 ω6	830:871	The major fatty acids were C16:0, C18:1 ω9c, Summed C19:1 ω6c/C19:0 cyclo ω10c/C19:0 ω6 and Summed C18:1 ω7c/C18:1 ω6c.
33620525	8	40	theme	Bombilactobacillus	1332:1349	arg1	sp					1357:1358	the name Bombilactobacillus apium sp	1323:1358	the name Bombilactobacillus apium sp	1323:1358	In conclusion, the description of phenotypic and genotypic properties support strain DCY120T as a novel species within the genus Bombilactobacillus, for which the name Bombilactobacillus apium sp.
33620525	2	41	theme	bacterial	185:193	arg1	DCY120T					203:209	DCY120T	203:209	DCY120T	203:209	A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain, DCY120T, was isolated from the gut of honeybee (Apis cerana) in Gyeonggi-do, South Korea.
33620525	2	41	theme	bacterial	185:193	arg1	strain					195:200	A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain	84:200	A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain	84:200	A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain, DCY120T, was isolated from the gut of honeybee (Apis cerana) in Gyeonggi-do, South Korea.
33620525	4	42	theme	threshold	735:743	arg1	%					757:757	95-96%	752:757	95-96%	752:757	The genome of strain DCY120T was sequenced and the average nucleotide identity (ANI) between strain DCY120T and the related Bombilactobacillus type strains were below the threshold value (95-96%) for species delineation.
33620525	4	42	theme	threshold	735:743	arg1	value					745:749	the threshold value	731:749	the threshold value (95-96%) for species delineation	731:782	The genome of strain DCY120T was sequenced and the average nucleotide identity (ANI) between strain DCY120T and the related Bombilactobacillus type strains were below the threshold value (95-96%) for species delineation.
33620525	4	43	theme	average	615:621	arg1	ANI					644:646	ANI	644:646	ANI	644:646	The genome of strain DCY120T was sequenced and the average nucleotide identity (ANI) between strain DCY120T and the related Bombilactobacillus type strains were below the threshold value (95-96%) for species delineation.
33620525	4	43	theme	average	615:621	arg1	identity					634:641	the average nucleotide identity	611:641	the average nucleotide identity (ANI) between strain DCY120T and the related Bombilactobacillus type strains	611:718	The genome of strain DCY120T was sequenced and the average nucleotide identity (ANI) between strain DCY120T and the related Bombilactobacillus type strains were below the threshold value (95-96%) for species delineation.
33620525	7	44	from	acids	1067:1071	arg1	peptidoglycan					1076:1088	peptidoglycan	1076:1088	peptidoglycan of strain DCY120T	1076:1106	The amino acids in peptidoglycan of strain DCY120T were lysine, alanine, glutamic acid, and aspartic acid.
33620525	4	45	theme	type	707:710	arg1	strains					712:718	the related Bombilactobacillus type strains	676:718	the related Bombilactobacillus type strains	676:718	The genome of strain DCY120T was sequenced and the average nucleotide identity (ANI) between strain DCY120T and the related Bombilactobacillus type strains were below the threshold value (95-96%) for species delineation.
33620525	3	46	theme	rRNA	535:538	arg1	analysis					554:561	16S rRNA gene sequence analysis	531:561	16S rRNA gene sequence analysis	531:561	Strain DCY120T belongs to the genus Bombilactobacillus and is moderately related to Bombilactobacillus mellis Hon2T (94.1% similarity), Bombilactobacillus bombi BTLCH M1/2T (93.8%), and Bombilactobacillus mellifer Bin4NT (93.5%) based on 16S rRNA gene sequence analysis.
33620525	2	47	from	gut	234:236	arg1	South					280:284	South	280:284	South	280:284	A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain, DCY120T, was isolated from the gut of honeybee (Apis cerana) in Gyeonggi-do, South Korea.
33620525	2	47	from	gut	234:236	arg1	Korea					286:290	Korea	286:290	Korea	286:290	A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain, DCY120T, was isolated from the gut of honeybee (Apis cerana) in Gyeonggi-do, South Korea.
33620525	6	48	theme	major	909:913	arg1	diphosphatidylglycerol					933:954	diphosphatidylglycerol	933:954	diphosphatidylglycerol (DPG)	933:960	The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), one glycolipid (GL), and one unidentified aminophospholipid (APL).
33620525	6	48	theme	major	909:913	arg1	lipids					921:926	The major polar lipids	905:926	The major polar lipids	905:926	The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), one glycolipid (GL), and one unidentified aminophospholipid (APL).
33620525	3	49	theme	gene	540:543	arg1	analysis					554:561	16S rRNA gene sequence analysis	531:561	16S rRNA gene sequence analysis	531:561	Strain DCY120T belongs to the genus Bombilactobacillus and is moderately related to Bombilactobacillus mellis Hon2T (94.1% similarity), Bombilactobacillus bombi BTLCH M1/2T (93.8%), and Bombilactobacillus mellifer Bin4NT (93.5%) based on 16S rRNA gene sequence analysis.
33620525	5	50	theme	major	789:793	arg1	C16:0					812:816	C16:0	812:816	C16:0	812:816	The major fatty acids were C16:0, C18:1 ω9c, Summed C19:1 ω6c/C19:0 cyclo ω10c/C19:0 ω6 and Summed C18:1 ω7c/C18:1 ω6c.
33620525	5	50	theme	major	789:793	arg1	acids					801:805	The major fatty acids	785:805	The major fatty acids	785:805	The major fatty acids were C16:0, C18:1 ω9c, Summed C19:1 ω6c/C19:0 cyclo ω10c/C19:0 ω6 and Summed C18:1 ω7c/C18:1 ω6c.
33620525	6	51	theme	polar	915:919	arg1	diphosphatidylglycerol					933:954	diphosphatidylglycerol	933:954	diphosphatidylglycerol (DPG)	933:960	The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), one glycolipid (GL), and one unidentified aminophospholipid (APL).
33620525	6	51	theme	polar	915:919	arg1	lipids					921:926	The major polar lipids	905:926	The major polar lipids	905:926	The major polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), one glycolipid (GL), and one unidentified aminophospholipid (APL).
33620525	7	52	theme	DCY120T	1100:1106	arg1	peptidoglycan					1076:1088	peptidoglycan	1076:1088	peptidoglycan of strain DCY120T	1076:1106	The amino acids in peptidoglycan of strain DCY120T were lysine, alanine, glutamic acid, and aspartic acid.
33620525	8	53	theme	genotypic	1213:1221	arg1	properties					1223:1232	phenotypic and genotypic properties	1198:1232	phenotypic and genotypic properties	1198:1232	In conclusion, the description of phenotypic and genotypic properties support strain DCY120T as a novel species within the genus Bombilactobacillus, for which the name Bombilactobacillus apium sp.
33620525	10	54	theme	type	1383:1386	arg1	DCY120T					1398:1404	DCY120T	1398:1404	DCY120T (= KCTC 43194T = JCM 34006T)	1398:1433	The type strain is DCY120T (= KCTC 43194T = JCM 34006T).
33620525	10	54	theme	type	1383:1386	arg1	strain					1388:1393	The type strain	1379:1393	The type strain	1379:1393	The type strain is DCY120T (= KCTC 43194T = JCM 34006T).
33620525	2	55	theme	Gram-reaction	92:104	arg1	DCY120T					203:209	DCY120T	203:209	DCY120T	203:209	A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain, DCY120T, was isolated from the gut of honeybee (Apis cerana) in Gyeonggi-do, South Korea.
33620525	2	55	theme	Gram-reaction	92:104	arg1	strain					195:200	A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain	84:200	A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain	84:200	A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain, DCY120T, was isolated from the gut of honeybee (Apis cerana) in Gyeonggi-do, South Korea.
33620525	3	56	theme	16S	531:533	arg1	analysis					554:561	16S rRNA gene sequence analysis	531:561	16S rRNA gene sequence analysis	531:561	Strain DCY120T belongs to the genus Bombilactobacillus and is moderately related to Bombilactobacillus mellis Hon2T (94.1% similarity), Bombilactobacillus bombi BTLCH M1/2T (93.8%), and Bombilactobacillus mellifer Bin4NT (93.5%) based on 16S rRNA gene sequence analysis.
33620525	0	57	theme	apium	19:23	arg1	sp					25:26	Bombilactobacillus apium sp	0:26	Bombilactobacillus apium sp.	0:27	Bombilactobacillus apium sp.
33620525	2	58	attach	isolated	216:223	arg2	strain					195:200	A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain	84:200	A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain	84:200	A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain, DCY120T, was isolated from the gut of honeybee (Apis cerana) in Gyeonggi-do, South Korea.
33620525	2	58	attach	isolated	216:223	arg1	gut					234:236	the gut	230:236	the gut of honeybee (Apis cerana) in Gyeonggi-do, South Korea	230:290	A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain, DCY120T, was isolated from the gut of honeybee (Apis cerana) in Gyeonggi-do, South Korea.
33620525	2	58	attach	isolated	216:223	arg2	DCY120T					203:209	DCY120T	203:209	DCY120T	203:209	A novel Gram-reaction positive-, catalase and oxidase negative-, rod-shaped, facultatively anaerobic bacterial strain, DCY120T, was isolated from the gut of honeybee (Apis cerana) in Gyeonggi-do, South Korea.
34112803	0	0	theme	receptor-expressing	89:107	arg1	cells					109:113	cytokine receptor-expressing cells	80:113	cytokine receptor-expressing cells	80:113	Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution.
34112803	6	1	theme	exosome-bound	1032:1044	arg1	determinant					1070:1080	a crucial determinant	1060:1080	a crucial determinant of exosome-cell interactions	1060:1109	Strikingly, CCL2-decorated exosomes are directed to a subset of cells that express the CCL2 receptor CCR2, demonstrating that exosome-bound cytokines are a crucial determinant of exosome-cell interactions.
34112803	6	1	theme	exosome-bound	1032:1044	arg1	cytokines					1046:1054	exosome-bound cytokines	1032:1054	exosome-bound cytokines	1032:1054	Strikingly, CCL2-decorated exosomes are directed to a subset of cells that express the CCL2 receptor CCR2, demonstrating that exosome-bound cytokines are a crucial determinant of exosome-cell interactions.
34112803	7	2	theme	patients	1209:1216	arg1	blood					1193:1197	the blood	1189:1197	the blood of cancer patients	1189:1216	In addition to the finding that cytokine-conjugated exosomes are detected in the blood of cancer patients, we discovered that healthy subjects derived exosomes are also associated with cytokines.
34112803	1	3	theme	secondary	169:177	arg1	sites					179:183	secondary sites	169:183	secondary sites	169:183	Metastatic spread of a cancer to secondary sites is a coordinated, non-random process.
34112803	0	4	theme	cytokine	80:87	arg1	cells					109:113	cytokine receptor-expressing cells	80:113	cytokine receptor-expressing cells	80:113	Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution.
34112803	8	5	theme	different	1330:1338	arg1	profile					1340:1346	a different profile	1328:1346	a different profile from exosomes isolated from cancer patients	1328:1390	Although displaying a different profile from exosomes isolated from cancer patients, it further indicates that specific combinations of cytokines bound to exosomes could likewise affect other physiological and disease settings.
34112803	6	6	theme	crucial	1062:1068	arg1	determinant					1070:1080	a crucial determinant	1060:1080	a crucial determinant of exosome-cell interactions	1060:1109	Strikingly, CCL2-decorated exosomes are directed to a subset of cells that express the CCL2 receptor CCR2, demonstrating that exosome-bound cytokines are a crucial determinant of exosome-cell interactions.
34112803	6	6	theme	crucial	1062:1068	arg1	cytokines					1046:1054	exosome-bound cytokines	1032:1054	exosome-bound cytokines	1032:1054	Strikingly, CCL2-decorated exosomes are directed to a subset of cells that express the CCL2 receptor CCR2, demonstrating that exosome-bound cytokines are a crucial determinant of exosome-cell interactions.
34112803	6	7	theme	cells	970:974	arg1	subset					960:965	a subset	958:965	a subset of cells that express the CCL2 receptor CCR2, demonstrating that exosome-bound cytokines are a crucial determinant of exosome-cell interactions	958:1109	Strikingly, CCL2-decorated exosomes are directed to a subset of cells that express the CCL2 receptor CCR2, demonstrating that exosome-bound cytokines are a crucial determinant of exosome-cell interactions.
34112803	7	8	theme	cancer	1202:1207	arg1	patients					1209:1216	cancer patients	1202:1216	cancer patients	1202:1216	In addition to the finding that cytokine-conjugated exosomes are detected in the blood of cancer patients, we discovered that healthy subjects derived exosomes are also associated with cytokines.
34112803	8	9	theme	cytokines	1444:1452	arg1	combinations					1428:1439	specific combinations	1419:1439	specific combinations of cytokines bound to exosomes	1419:1470	Although displaying a different profile from exosomes isolated from cancer patients, it further indicates that specific combinations of cytokines bound to exosomes could likewise affect other physiological and disease settings.
34112803	1	10	theme	Metastatic	136:145	arg1	process					214:220	a coordinated, non-random process	188:220	a coordinated, non-random process	188:220	Metastatic spread of a cancer to secondary sites is a coordinated, non-random process.
34112803	1	10	theme	Metastatic	136:145	arg1	spread					147:152	Metastatic spread	136:152	Metastatic spread of a cancer to secondary sites	136:183	Metastatic spread of a cancer to secondary sites is a coordinated, non-random process.
34112803	8	11	attach	isolated	1362:1369	arg2	exosomes					1353:1360	exosomes	1353:1360	exosomes isolated from cancer patients	1353:1390	Although displaying a different profile from exosomes isolated from cancer patients, it further indicates that specific combinations of cytokines bound to exosomes could likewise affect other physiological and disease settings.
34112803	8	11	attach	isolated	1362:1369	arg1	patients					1383:1390	cancer patients	1376:1390	cancer patients	1376:1390	Although displaying a different profile from exosomes isolated from cancer patients, it further indicates that specific combinations of cytokines bound to exosomes could likewise affect other physiological and disease settings.
34112803	1	12	theme	coordinated	190:200	arg1	process					214:220	a coordinated, non-random process	188:220	a coordinated, non-random process	188:220	Metastatic spread of a cancer to secondary sites is a coordinated, non-random process.
34112803	1	12	theme	coordinated	190:200	arg1	spread					147:152	Metastatic spread	136:152	Metastatic spread of a cancer to secondary sites	136:183	Metastatic spread of a cancer to secondary sites is a coordinated, non-random process.
34112803	4	13	theme	surface	661:667	arg1	chains					692:697	surface glycosaminoglycan side chains	661:697	surface glycosaminoglycan side chains of proteoglycans	661:714	Here, we show that microenvironmental cytokines, particularly CCL2, decorate cancer exosomes via binding to surface glycosaminoglycan side chains of proteoglycans, causing exosome accumulation in specific cell subsets and organs.
34112803	4	14	from	accumulation	733:744	arg1	organs					775:780	organs	775:780	organs	775:780	Here, we show that microenvironmental cytokines, particularly CCL2, decorate cancer exosomes via binding to surface glycosaminoglycan side chains of proteoglycans, causing exosome accumulation in specific cell subsets and organs.
34112803	4	14	from	accumulation	733:744	arg1	subsets					763:769	specific cell subsets	749:769	specific cell subsets	749:769	Here, we show that microenvironmental cytokines, particularly CCL2, decorate cancer exosomes via binding to surface glycosaminoglycan side chains of proteoglycans, causing exosome accumulation in specific cell subsets and organs.
34112803	2	15	theme	localization	427:438	arg1	aspects					401:407	some aspects	396:407	some aspects of organ-specific localization	396:438	Cancer cell-secreted vesicles, especially exosomes, have recently been implicated in the guidance of metastatic dissemination, with specific surface composition determining some aspects of organ-specific localization.
34112803	0	16	theme	microenvironmental	6:23	arg1	cytokines					25:33	Tumor microenvironmental cytokines	0:33	Tumor microenvironmental cytokines bound to cancer exosomes	0:58	Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution.
34112803	2	17	theme	dissemination	335:347	arg1	guidance					312:319	the guidance	308:319	the guidance of metastatic dissemination	308:347	Cancer cell-secreted vesicles, especially exosomes, have recently been implicated in the guidance of metastatic dissemination, with specific surface composition determining some aspects of organ-specific localization.
34112803	3	18	theme	tumor	467:471	arg1	microenvironment					473:488	the tumor microenvironment	463:488	the tumor microenvironment	463:488	Nevertheless, whether the tumor microenvironment influences exosome biodistribution has yet to be investigated.
34112803	0	19	theme	Tumor	0:4	arg1	cytokines					25:33	Tumor microenvironmental cytokines	0:33	Tumor microenvironmental cytokines bound to cancer exosomes	0:58	Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution.
34112803	2	20	theme	metastatic	324:333	arg1	dissemination					335:347	metastatic dissemination	324:347	metastatic dissemination	324:347	Cancer cell-secreted vesicles, especially exosomes, have recently been implicated in the guidance of metastatic dissemination, with specific surface composition determining some aspects of organ-specific localization.
34112803	5	21	theme	metastatic	887:896	arg1	burden					898:903	a higher metastatic burden	878:903	a higher metastatic burden	878:903	Exosome retention results in changes in the immune landscape within these organs, coupled with a higher metastatic burden.
34112803	5	22	theme	higher	880:885	arg1	burden					898:903	a higher metastatic burden	878:903	a higher metastatic burden	878:903	Exosome retention results in changes in the immune landscape within these organs, coupled with a higher metastatic burden.
34112803	2	23	theme	organ-specific	412:425	arg1	localization					427:438	organ-specific localization	412:438	organ-specific localization	412:438	Cancer cell-secreted vesicles, especially exosomes, have recently been implicated in the guidance of metastatic dissemination, with specific surface composition determining some aspects of organ-specific localization.
34112803	4	24	theme	proteoglycans	702:714	arg1	chains					692:697	surface glycosaminoglycan side chains	661:697	surface glycosaminoglycan side chains of proteoglycans	661:714	Here, we show that microenvironmental cytokines, particularly CCL2, decorate cancer exosomes via binding to surface glycosaminoglycan side chains of proteoglycans, causing exosome accumulation in specific cell subsets and organs.
34112803	4	25	theme	cancer	630:635	arg1	exosomes					637:644	cancer exosomes	630:644	cancer exosomes	630:644	Here, we show that microenvironmental cytokines, particularly CCL2, decorate cancer exosomes via binding to surface glycosaminoglycan side chains of proteoglycans, causing exosome accumulation in specific cell subsets and organs.
34112803	5	26	theme	Exosome	783:789	arg1	retention					791:799	Exosome retention	783:799	Exosome retention	783:799	Exosome retention results in changes in the immune landscape within these organs, coupled with a higher metastatic burden.
34112803	6	27	theme	CCL2-decorated	918:931	arg1	exosomes					933:940	CCL2-decorated exosomes	918:940	CCL2-decorated exosomes	918:940	Strikingly, CCL2-decorated exosomes are directed to a subset of cells that express the CCL2 receptor CCR2, demonstrating that exosome-bound cytokines are a crucial determinant of exosome-cell interactions.
34112803	8	28	theme	specific	1419:1426	arg1	combinations					1428:1439	specific combinations	1419:1439	specific combinations of cytokines bound to exosomes	1419:1470	Although displaying a different profile from exosomes isolated from cancer patients, it further indicates that specific combinations of cytokines bound to exosomes could likewise affect other physiological and disease settings.
34112803	0	29	theme	cancer	44:49	arg1	exosomes					51:58	cancer exosomes	44:58	cancer exosomes	44:58	Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution.
34112803	4	30	theme	specific	749:756	arg1	subsets					763:769	specific cell subsets	749:769	specific cell subsets	749:769	Here, we show that microenvironmental cytokines, particularly CCL2, decorate cancer exosomes via binding to surface glycosaminoglycan side chains of proteoglycans, causing exosome accumulation in specific cell subsets and organs.
34112803	1	31	dep	coordinated	190:200	arg1	non-random					203:212	non-random	203:212	non-random	203:212	Metastatic spread of a cancer to secondary sites is a coordinated, non-random process.
34112803	7	32	located	detected	1177:1184	arg2	exosomes					1164:1171	cytokine-conjugated exosomes	1144:1171	cytokine-conjugated exosomes	1144:1171	In addition to the finding that cytokine-conjugated exosomes are detected in the blood of cancer patients, we discovered that healthy subjects derived exosomes are also associated with cytokines.
34112803	7	32	located	detected	1177:1184	arg1	blood					1193:1197	the blood	1189:1197	the blood of cancer patients	1189:1216	In addition to the finding that cytokine-conjugated exosomes are detected in the blood of cancer patients, we discovered that healthy subjects derived exosomes are also associated with cytokines.
34112803	7	33	theme	healthy	1238:1244	arg1	subjects					1246:1253	healthy subjects	1238:1253	healthy subjects derived exosomes	1238:1270	In addition to the finding that cytokine-conjugated exosomes are detected in the blood of cancer patients, we discovered that healthy subjects derived exosomes are also associated with cytokines.
34112803	7	34	theme	cytokine-conjugated	1144:1162	arg1	exosomes					1164:1171	cytokine-conjugated exosomes	1144:1171	cytokine-conjugated exosomes	1144:1171	In addition to the finding that cytokine-conjugated exosomes are detected in the blood of cancer patients, we discovered that healthy subjects derived exosomes are also associated with cytokines.
34112803	8	35	from	exosomes	1353:1360	arg1	profile					1340:1346	a different profile	1328:1346	a different profile from exosomes isolated from cancer patients	1328:1390	Although displaying a different profile from exosomes isolated from cancer patients, it further indicates that specific combinations of cytokines bound to exosomes could likewise affect other physiological and disease settings.
34112803	8	36	theme	cancer	1376:1381	arg1	patients					1383:1390	cancer patients	1376:1390	cancer patients	1376:1390	Although displaying a different profile from exosomes isolated from cancer patients, it further indicates that specific combinations of cytokines bound to exosomes could likewise affect other physiological and disease settings.
34112803	4	37	theme	microenvironmental	572:589	arg1	cytokines					591:599	microenvironmental cytokines	572:599	microenvironmental cytokines	572:599	Here, we show that microenvironmental cytokines, particularly CCL2, decorate cancer exosomes via binding to surface glycosaminoglycan side chains of proteoglycans, causing exosome accumulation in specific cell subsets and organs.
34112803	4	37	theme	microenvironmental	572:589	arg1	CCL2					615:618	CCL2	615:618	particularly CCL2	602:618	Here, we show that microenvironmental cytokines, particularly CCL2, decorate cancer exosomes via binding to surface glycosaminoglycan side chains of proteoglycans, causing exosome accumulation in specific cell subsets and organs.
34112803	5	38	from	changes	812:818	arg1	landscape					834:842	the immune landscape	823:842	the immune landscape	823:842	Exosome retention results in changes in the immune landscape within these organs, coupled with a higher metastatic burden.
34112803	2	39	theme	surface	364:370	arg1	composition					372:382	specific surface composition	355:382	specific surface composition determining some aspects of organ-specific localization	355:438	Cancer cell-secreted vesicles, especially exosomes, have recently been implicated in the guidance of metastatic dissemination, with specific surface composition determining some aspects of organ-specific localization.
34112803	2	40	theme	Cancer	223:228	arg1	vesicles					244:251	Cancer cell-secreted vesicles	223:251	Cancer cell-secreted vesicles	223:251	Cancer cell-secreted vesicles, especially exosomes, have recently been implicated in the guidance of metastatic dissemination, with specific surface composition determining some aspects of organ-specific localization.
34112803	2	40	theme	Cancer	223:228	arg1	exosomes					265:272	exosomes	265:272	especially exosomes	254:272	Cancer cell-secreted vesicles, especially exosomes, have recently been implicated in the guidance of metastatic dissemination, with specific surface composition determining some aspects of organ-specific localization.
34112803	2	41	theme	cell-secreted	230:242	arg1	vesicles					244:251	Cancer cell-secreted vesicles	223:251	Cancer cell-secreted vesicles	223:251	Cancer cell-secreted vesicles, especially exosomes, have recently been implicated in the guidance of metastatic dissemination, with specific surface composition determining some aspects of organ-specific localization.
34112803	2	41	theme	cell-secreted	230:242	arg1	exosomes					265:272	exosomes	265:272	especially exosomes	254:272	Cancer cell-secreted vesicles, especially exosomes, have recently been implicated in the guidance of metastatic dissemination, with specific surface composition determining some aspects of organ-specific localization.
34112803	2	42	theme	specific	355:362	arg1	composition					372:382	specific surface composition	355:382	specific surface composition determining some aspects of organ-specific localization	355:438	Cancer cell-secreted vesicles, especially exosomes, have recently been implicated in the guidance of metastatic dissemination, with specific surface composition determining some aspects of organ-specific localization.
34112803	6	43	theme	receptor	998:1005	arg1	CCR2					1007:1010	the CCL2 receptor CCR2	989:1010	the CCL2 receptor CCR2	989:1010	Strikingly, CCL2-decorated exosomes are directed to a subset of cells that express the CCL2 receptor CCR2, demonstrating that exosome-bound cytokines are a crucial determinant of exosome-cell interactions.
34112803	4	44	theme	glycosaminoglycan	669:685	arg1	chains					692:697	surface glycosaminoglycan side chains	661:697	surface glycosaminoglycan side chains of proteoglycans	661:714	Here, we show that microenvironmental cytokines, particularly CCL2, decorate cancer exosomes via binding to surface glycosaminoglycan side chains of proteoglycans, causing exosome accumulation in specific cell subsets and organs.
34112803	3	45	theme	exosome	501:507	arg1	biodistribution					509:523	exosome biodistribution	501:523	exosome biodistribution	501:523	Nevertheless, whether the tumor microenvironment influences exosome biodistribution has yet to be investigated.
34112803	5	46	theme	immune	827:832	arg1	landscape					834:842	the immune landscape	823:842	the immune landscape	823:842	Exosome retention results in changes in the immune landscape within these organs, coupled with a higher metastatic burden.
34112803	1	47	theme	cancer	159:164	arg1	process					214:220	a coordinated, non-random process	188:220	a coordinated, non-random process	188:220	Metastatic spread of a cancer to secondary sites is a coordinated, non-random process.
34112803	1	47	theme	cancer	159:164	arg1	spread					147:152	Metastatic spread	136:152	Metastatic spread of a cancer to secondary sites	136:183	Metastatic spread of a cancer to secondary sites is a coordinated, non-random process.
34112803	4	48	theme	exosome	725:731	arg1	accumulation					733:744	exosome accumulation	725:744	exosome accumulation in specific cell subsets and organs	725:780	Here, we show that microenvironmental cytokines, particularly CCL2, decorate cancer exosomes via binding to surface glycosaminoglycan side chains of proteoglycans, causing exosome accumulation in specific cell subsets and organs.
34112803	6	49	theme	interactions	1098:1109	arg1	determinant					1070:1080	a crucial determinant	1060:1080	a crucial determinant of exosome-cell interactions	1060:1109	Strikingly, CCL2-decorated exosomes are directed to a subset of cells that express the CCL2 receptor CCR2, demonstrating that exosome-bound cytokines are a crucial determinant of exosome-cell interactions.
34112803	6	49	theme	interactions	1098:1109	arg1	cytokines					1046:1054	exosome-bound cytokines	1032:1054	exosome-bound cytokines	1032:1054	Strikingly, CCL2-decorated exosomes are directed to a subset of cells that express the CCL2 receptor CCR2, demonstrating that exosome-bound cytokines are a crucial determinant of exosome-cell interactions.
34112803	6	50	theme	CCL2	993:996	arg1	CCR2					1007:1010	the CCL2 receptor CCR2	989:1010	the CCL2 receptor CCR2	989:1010	Strikingly, CCL2-decorated exosomes are directed to a subset of cells that express the CCL2 receptor CCR2, demonstrating that exosome-bound cytokines are a crucial determinant of exosome-cell interactions.
34112803	6	51	theme	exosome-cell	1085:1096	arg1	interactions					1098:1109	exosome-cell interactions	1085:1109	exosome-cell interactions	1085:1109	Strikingly, CCL2-decorated exosomes are directed to a subset of cells that express the CCL2 receptor CCR2, demonstrating that exosome-bound cytokines are a crucial determinant of exosome-cell interactions.
34112803	4	52	theme	cell	758:761	arg1	subsets					763:769	specific cell subsets	749:769	specific cell subsets	749:769	Here, we show that microenvironmental cytokines, particularly CCL2, decorate cancer exosomes via binding to surface glycosaminoglycan side chains of proteoglycans, causing exosome accumulation in specific cell subsets and organs.
34112803	8	53	theme	disease	1518:1524	arg1	settings					1526:1533	disease settings	1518:1533	disease settings	1518:1533	Although displaying a different profile from exosomes isolated from cancer patients, it further indicates that specific combinations of cytokines bound to exosomes could likewise affect other physiological and disease settings.
34112803	4	54	theme	side	687:690	arg1	chains					692:697	surface glycosaminoglycan side chains	661:697	surface glycosaminoglycan side chains of proteoglycans	661:714	Here, we show that microenvironmental cytokines, particularly CCL2, decorate cancer exosomes via binding to surface glycosaminoglycan side chains of proteoglycans, causing exosome accumulation in specific cell subsets and organs.
33099259	11	0	theme	type	1810:1813	arg1	nov					1804:1806	nov	1804:1806	nov	1804:1806	nov. (type strain K478T =JCM 33652T =CGMCC 1.17229T) are proposed.
33099259	11	0	theme	type	1810:1813	arg1	1.17229T					1847:1854	type strain K478T =JCM 33652T =CGMCC 1.17229T	1810:1854	type strain K478T =JCM 33652T =CGMCC 1.17229T	1810:1854	nov. (type strain K478T =JCM 33652T =CGMCC 1.17229T) are proposed.
33099259	9	1	theme	novel	1631:1635	arg1	species					1637:1643	two novel species	1627:1643	two novel species	1627:1643	The obtained results demonstrated that strains E257T and K478T represent two novel species of the genus Motilibacter, for which the names Motilibacter desertisp.
33099259	7	2	theme	Conserved	1041:1049	arg1	POCP					1061:1064	POCP	1061:1064	POCP	1061:1064	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	7	2	theme	Conserved	1041:1049	arg1	Proteins					1051:1058	Conserved Proteins	1041:1058	Conserved Proteins (POCP) calculated from the complete genome sequences	1041:1111	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	9	3	theme	genus	1652:1656	arg1	Motilibacter					1658:1669	the genus Motilibacter	1648:1669	the genus Motilibacter	1648:1669	The obtained results demonstrated that strains E257T and K478T represent two novel species of the genus Motilibacter, for which the names Motilibacter desertisp.
33099259	4	4	theme	16S	358:360	arg1	genes					367:371	16S rRNA genes	358:371	16S rRNA genes	358:371	Comparative analysis of 16S rRNA genes showed that strains E257T and K478T were assigned to the genus Motilibacter, being their closest relative M. rhizosphaerae RS-16T with 97.3% and 96.7% similarities, respectively.
33099259	10	5	theme	=	1740:1740	arg1	nov					1716:1718	nov	1716:1718	nov	1716:1718	nov. (type strain E257T = JCM 33651T = CGMCC 1.17159T) and Motilibacter aurantiacus sp.
33099259	10	5	theme	=	1740:1740	arg1	1.17159T					1761:1768	type strain E257T = JCM 33651T = CGMCC 1.17159T	1722:1768	type strain E257T = JCM 33651T = CGMCC 1.17159T	1722:1768	nov. (type strain E257T = JCM 33651T = CGMCC 1.17159T) and Motilibacter aurantiacus sp.
33099259	4	6	theme	genes	367:371	arg1	analysis					346:353	Comparative analysis	334:353	Comparative analysis of 16S rRNA genes	334:371	Comparative analysis of 16S rRNA genes showed that strains E257T and K478T were assigned to the genus Motilibacter, being their closest relative M. rhizosphaerae RS-16T with 97.3% and 96.7% similarities, respectively.
33099259	10	7	theme	strain	1727:1732	arg1	nov					1716:1718	nov	1716:1718	nov	1716:1718	nov. (type strain E257T = JCM 33651T = CGMCC 1.17159T) and Motilibacter aurantiacus sp.
33099259	10	7	theme	strain	1727:1732	arg1	1.17159T					1761:1768	type strain E257T = JCM 33651T = CGMCC 1.17159T	1722:1768	type strain E257T = JCM 33651T = CGMCC 1.17159T	1722:1768	nov. (type strain E257T = JCM 33651T = CGMCC 1.17159T) and Motilibacter aurantiacus sp.
33099259	9	8	dep	strains	1593:1599	arg1	E257T					1601:1605	E257T	1601:1605	E257T	1601:1605	The obtained results demonstrated that strains E257T and K478T represent two novel species of the genus Motilibacter, for which the names Motilibacter desertisp.
33099259	9	8	dep	strains	1593:1599	arg1	strains					1593:1599	strains	1593:1599	strains E257T and K478T	1593:1615	The obtained results demonstrated that strains E257T and K478T represent two novel species of the genus Motilibacter, for which the names Motilibacter desertisp.
33099259	9	8	dep	strains	1593:1599	arg1	K478T					1611:1615	K478T	1611:1615	K478T	1611:1615	The obtained results demonstrated that strains E257T and K478T represent two novel species of the genus Motilibacter, for which the names Motilibacter desertisp.
33099259	10	9	theme	CGMCC	1755:1759	arg1	nov					1716:1718	nov	1716:1718	nov	1716:1718	nov. (type strain E257T = JCM 33651T = CGMCC 1.17159T) and Motilibacter aurantiacus sp.
33099259	10	9	theme	CGMCC	1755:1759	arg1	1.17159T					1761:1768	type strain E257T = JCM 33651T = CGMCC 1.17159T	1722:1768	type strain E257T = JCM 33651T = CGMCC 1.17159T	1722:1768	nov. (type strain E257T = JCM 33651T = CGMCC 1.17159T) and Motilibacter aurantiacus sp.
33099259	2	10	theme	novel	73:77	arg1	nov.					63:66	nov.	63:66	nov.	63:66	nov., two novel actinobacteria isolated from soil of Cholistan Desert and emended description of the genus Motilibacter.
33099259	2	10	theme	novel	73:77	arg1	actinobacteria					79:92	two novel actinobacteria	69:92	two novel actinobacteria	69:92	nov., two novel actinobacteria isolated from soil of Cholistan Desert and emended description of the genus Motilibacter.
33099259	7	11	theme	hybridization	995:1007	arg1	values					1016:1021	digital DNA-DNA hybridization (dDDH) values	979:1021	digital DNA-DNA hybridization (dDDH) values	979:1021	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	10	12	theme	33651T	1746:1751	arg1	nov					1716:1718	nov	1716:1718	nov	1716:1718	nov. (type strain E257T = JCM 33651T = CGMCC 1.17159T) and Motilibacter aurantiacus sp.
33099259	10	12	theme	33651T	1746:1751	arg1	1.17159T					1761:1768	type strain E257T = JCM 33651T = CGMCC 1.17159T	1722:1768	type strain E257T = JCM 33651T = CGMCC 1.17159T	1722:1768	nov. (type strain E257T = JCM 33651T = CGMCC 1.17159T) and Motilibacter aurantiacus sp.
33099259	6	13	theme	Motilibacter	895:906	arg1	species					874:880	the two type species	861:880	the two type species of the genus Motilibacter	861:906	Phylogenetic analysis based on 16S rRNA gene sequences and phylogenomic analysis based on multiple genes of conserved core proteins exhibited that these two strains belonged to the genus Motilibacter and formed a robust cluster separated from the two type species of the genus Motilibacter.
33099259	3	14	theme	soil	280:283	arg1	samples					285:291	hyper-arid soil samples	269:291	hyper-arid soil samples collected in Cholistan Desert, Pakistan	269:331	Two novel actinobacterial strains, designated as E257T and K478T, were isolated from hyper-arid soil samples collected in Cholistan Desert, Pakistan.
33099259	1	15	dep	sp	59:60	arg1	aurantiacus					47:57	nov. and Motilibacter aurantiacus	25:57	nov. and Motilibacter aurantiacus	25:57	nov. and Motilibacter aurantiacus sp.
33099259	7	16	theme	digital	979:985	arg1	dDDH					1010:1013	dDDH	1010:1013	dDDH	1010:1013	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	7	16	theme	digital	979:985	arg1	hybridization					995:1007	digital DNA-DNA hybridization	979:1007	digital DNA-DNA hybridization (dDDH) values	979:1021	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	7	17	theme	Amino	952:956	arg1	AAI					973:975	AAI	973:975	AAI	973:975	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	7	17	theme	Amino	952:956	arg1	Identity					963:970	Average Amino acid Identity	944:970	Average Amino acid Identity (AAI)	944:976	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	4	18	theme	M.	479:480	arg1	RS-16T					496:501	their closest relative M. rhizosphaerae RS-16T	456:501	their closest relative M. rhizosphaerae RS-16T	456:501	Comparative analysis of 16S rRNA genes showed that strains E257T and K478T were assigned to the genus Motilibacter, being their closest relative M. rhizosphaerae RS-16T with 97.3% and 96.7% similarities, respectively.
33099259	6	19	theme	phylogenomic	677:688	arg1	analysis					690:697	phylogenomic analysis	677:697	phylogenomic analysis based on multiple genes of conserved core proteins	677:748	Phylogenetic analysis based on 16S rRNA gene sequences and phylogenomic analysis based on multiple genes of conserved core proteins exhibited that these two strains belonged to the genus Motilibacter and formed a robust cluster separated from the two type species of the genus Motilibacter.
33099259	11	20	theme	=CGMCC	1840:1845	arg1	nov					1804:1806	nov	1804:1806	nov	1804:1806	nov. (type strain K478T =JCM 33652T =CGMCC 1.17229T) are proposed.
33099259	11	20	theme	=CGMCC	1840:1845	arg1	1.17229T					1847:1854	type strain K478T =JCM 33652T =CGMCC 1.17229T	1810:1854	type strain K478T =JCM 33652T =CGMCC 1.17229T	1810:1854	nov. (type strain K478T =JCM 33652T =CGMCC 1.17229T) are proposed.
33099259	7	21	theme	Average	909:915	arg1	ANI					938:940	ANI	938:940	ANI	938:940	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	7	21	theme	Average	909:915	arg1	Identity					928:935	Average Nucleotide Identity	909:935	Average Nucleotide Identity (ANI)	909:941	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	8	22	contain	have	1365:1368	arg1	isolates					1342:1349	The two isolates	1334:1349	The two isolates	1334:1349	The two isolates were found to have chemotaxonomic, cultural and morphological properties consistent with their classification in the genus Motilibacter and also confirmed the differentiation from their closest species.
33099259	8	22	contain	have	1365:1368	arg2	properties					1413:1422	chemotaxonomic, cultural and morphological properties	1370:1422	chemotaxonomic, cultural and morphological properties consistent with their classification in the genus Motilibacter	1370:1485	The two isolates were found to have chemotaxonomic, cultural and morphological properties consistent with their classification in the genus Motilibacter and also confirmed the differentiation from their closest species.
33099259	4	23	theme	closest	462:468	arg1	RS-16T					496:501	their closest relative M. rhizosphaerae RS-16T	456:501	their closest relative M. rhizosphaerae RS-16T	456:501	Comparative analysis of 16S rRNA genes showed that strains E257T and K478T were assigned to the genus Motilibacter, being their closest relative M. rhizosphaerae RS-16T with 97.3% and 96.7% similarities, respectively.
33099259	3	24	theme	actinobacterial	194:208	arg1	strains					210:216	Two novel actinobacterial strains	184:216	Two novel actinobacterial strains	184:216	Two novel actinobacterial strains, designated as E257T and K478T, were isolated from hyper-arid soil samples collected in Cholistan Desert, Pakistan.
33099259	11	25	theme	=JCM	1828:1831	arg1	nov					1804:1806	nov	1804:1806	nov	1804:1806	nov. (type strain K478T =JCM 33652T =CGMCC 1.17229T) are proposed.
33099259	11	25	theme	=JCM	1828:1831	arg1	1.17229T					1847:1854	type strain K478T =JCM 33652T =CGMCC 1.17229T	1810:1854	type strain K478T =JCM 33652T =CGMCC 1.17229T	1810:1854	nov. (type strain K478T =JCM 33652T =CGMCC 1.17229T) are proposed.
33099259	0	26	theme	Motilibacter	0:11	arg1	sp					21:22	Motilibacter deserti sp	0:22	Motilibacter deserti sp.	0:23	Motilibacter deserti sp.
33099259	5	27	theme	sequence	556:563	arg1	similarity					565:574	The sequence similarity	552:574	The sequence similarity between strain E257T and K478T	552:605	The sequence similarity between strain E257T and K478T was 98.9%.
33099259	5	27	theme	sequence	556:563	arg1	%					615:615	98.9%	611:615	98.9%	611:615	The sequence similarity between strain E257T and K478T was 98.9%.
33099259	7	28	theme	genus	1257:1261	arg1	Motilibacter					1263:1274	the genus Motilibacter	1253:1274	the genus Motilibacter	1253:1274	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	6	29	theme	rRNA	653:656	arg1	sequences					663:671	16S rRNA gene sequences	649:671	16S rRNA gene sequences	649:671	Phylogenetic analysis based on 16S rRNA gene sequences and phylogenomic analysis based on multiple genes of conserved core proteins exhibited that these two strains belonged to the genus Motilibacter and formed a robust cluster separated from the two type species of the genus Motilibacter.
33099259	8	30	from	classification	1446:1459	arg1	Motilibacter					1474:1485	the genus Motilibacter	1464:1485	the genus Motilibacter	1464:1485	The two isolates were found to have chemotaxonomic, cultural and morphological properties consistent with their classification in the genus Motilibacter and also confirmed the differentiation from their closest species.
33099259	2	31	theme	genus	164:168	arg1	Motilibacter					170:181	the genus Motilibacter	160:181	the genus Motilibacter	160:181	nov., two novel actinobacteria isolated from soil of Cholistan Desert and emended description of the genus Motilibacter.
33099259	7	32	theme	complete	1087:1094	arg1	sequences					1103:1111	the complete genome sequences	1083:1111	the complete genome sequences	1083:1111	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	6	33	theme	robust	831:836	arg1	cluster					838:844	a robust cluster	829:844	a robust cluster separated from the two type species of the genus Motilibacter	829:906	Phylogenetic analysis based on 16S rRNA gene sequences and phylogenomic analysis based on multiple genes of conserved core proteins exhibited that these two strains belonged to the genus Motilibacter and formed a robust cluster separated from the two type species of the genus Motilibacter.
33099259	4	34	theme	Comparative	334:344	arg1	analysis					346:353	Comparative analysis	334:353	Comparative analysis of 16S rRNA genes	334:371	Comparative analysis of 16S rRNA genes showed that strains E257T and K478T were assigned to the genus Motilibacter, being their closest relative M. rhizosphaerae RS-16T with 97.3% and 96.7% similarities, respectively.
33099259	7	35	theme	other	1236:1240	arg1	species					1242:1248	the other species	1232:1248	the other species of the genus Motilibacter	1232:1274	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	8	36	theme	closest	1537:1543	arg1	species					1545:1551	their closest species	1531:1551	their closest species	1531:1551	The two isolates were found to have chemotaxonomic, cultural and morphological properties consistent with their classification in the genus Motilibacter and also confirmed the differentiation from their closest species.
33099259	6	37	theme	core	736:739	arg1	proteins					741:748	conserved core proteins	726:748	conserved core proteins	726:748	Phylogenetic analysis based on 16S rRNA gene sequences and phylogenomic analysis based on multiple genes of conserved core proteins exhibited that these two strains belonged to the genus Motilibacter and formed a robust cluster separated from the two type species of the genus Motilibacter.
33099259	8	38	theme	chemotaxonomic	1370:1383	arg1	properties					1413:1422	chemotaxonomic, cultural and morphological properties	1370:1422	chemotaxonomic, cultural and morphological properties consistent with their classification in the genus Motilibacter	1370:1485	The two isolates were found to have chemotaxonomic, cultural and morphological properties consistent with their classification in the genus Motilibacter and also confirmed the differentiation from their closest species.
33099259	6	39	theme	multiple	708:715	arg1	genes					717:721	multiple genes	708:721	multiple genes of conserved core proteins	708:748	Phylogenetic analysis based on 16S rRNA gene sequences and phylogenomic analysis based on multiple genes of conserved core proteins exhibited that these two strains belonged to the genus Motilibacter and formed a robust cluster separated from the two type species of the genus Motilibacter.
33099259	11	40	theme	strain	1815:1820	arg1	nov					1804:1806	nov	1804:1806	nov	1804:1806	nov. (type strain K478T =JCM 33652T =CGMCC 1.17229T) are proposed.
33099259	11	40	theme	strain	1815:1820	arg1	1.17229T					1847:1854	type strain K478T =JCM 33652T =CGMCC 1.17229T	1810:1854	type strain K478T =JCM 33652T =CGMCC 1.17229T	1810:1854	nov. (type strain K478T =JCM 33652T =CGMCC 1.17229T) are proposed.
33099259	6	41	theme	genus	889:893	arg1	Motilibacter					895:906	the genus Motilibacter	885:906	the genus Motilibacter	885:906	Phylogenetic analysis based on 16S rRNA gene sequences and phylogenomic analysis based on multiple genes of conserved core proteins exhibited that these two strains belonged to the genus Motilibacter and formed a robust cluster separated from the two type species of the genus Motilibacter.
33099259	8	42	with	consistent	1424:1433	arg1	classification					1446:1459	their classification	1440:1459	their classification in the genus Motilibacter	1440:1485	The two isolates were found to have chemotaxonomic, cultural and morphological properties consistent with their classification in the genus Motilibacter and also confirmed the differentiation from their closest species.
33099259	10	43	dep	sp	1800:1801	arg1	nov					1716:1718	nov	1716:1718	nov	1716:1718	nov. (type strain E257T = JCM 33651T = CGMCC 1.17159T) and Motilibacter aurantiacus sp.
33099259	10	43	dep	sp	1800:1801	arg1	1.17159T					1761:1768	type strain E257T = JCM 33651T = CGMCC 1.17159T	1722:1768	type strain E257T = JCM 33651T = CGMCC 1.17159T	1722:1768	nov. (type strain E257T = JCM 33651T = CGMCC 1.17159T) and Motilibacter aurantiacus sp.
33099259	10	43	dep	sp	1800:1801	arg1	Motilibacter					1775:1786	Motilibacter	1775:1786	Motilibacter	1775:1786	nov. (type strain E257T = JCM 33651T = CGMCC 1.17159T) and Motilibacter aurantiacus sp.
33099259	7	44	theme	Proteins	1051:1058	arg1	Identity					928:935	Average Nucleotide Identity	909:935	Average Nucleotide Identity (ANI)	909:941	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	7	44	theme	Proteins	1051:1058	arg1	ANI					938:940	ANI	938:940	ANI	938:940	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	7	44	theme	Proteins	1051:1058	arg1	Percentage					1027:1036	Percentage	1027:1036	Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences	1027:1111	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	7	44	theme	Proteins	1051:1058	arg1	Identity					963:970	Average Amino acid Identity	944:970	Average Amino acid Identity (AAI)	944:976	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	7	44	theme	Proteins	1051:1058	arg1	AAI					973:975	AAI	973:975	AAI	973:975	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	7	44	theme	Proteins	1051:1058	arg1	values					1016:1021	digital DNA-DNA hybridization (dDDH) values	979:1021	digital DNA-DNA hybridization (dDDH) values	979:1021	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	10	45	theme	type	1722:1725	arg1	nov					1716:1718	nov	1716:1718	nov	1716:1718	nov. (type strain E257T = JCM 33651T = CGMCC 1.17159T) and Motilibacter aurantiacus sp.
33099259	10	45	theme	type	1722:1725	arg1	1.17159T					1761:1768	type strain E257T = JCM 33651T = CGMCC 1.17159T	1722:1768	type strain E257T = JCM 33651T = CGMCC 1.17159T	1722:1768	nov. (type strain E257T = JCM 33651T = CGMCC 1.17159T) and Motilibacter aurantiacus sp.
33099259	6	46	theme	type	869:872	arg1	species					874:880	the two type species	861:880	the two type species of the genus Motilibacter	861:906	Phylogenetic analysis based on 16S rRNA gene sequences and phylogenomic analysis based on multiple genes of conserved core proteins exhibited that these two strains belonged to the genus Motilibacter and formed a robust cluster separated from the two type species of the genus Motilibacter.
33099259	2	47	theme	emended	137:143	arg1	description					145:155	emended description	137:155	emended description of the genus Motilibacter	137:181	nov., two novel actinobacteria isolated from soil of Cholistan Desert and emended description of the genus Motilibacter.
33099259	4	48	theme	rRNA	362:365	arg1	genes					367:371	16S rRNA genes	358:371	16S rRNA genes	358:371	Comparative analysis of 16S rRNA genes showed that strains E257T and K478T were assigned to the genus Motilibacter, being their closest relative M. rhizosphaerae RS-16T with 97.3% and 96.7% similarities, respectively.
33099259	2	49	theme	Desert	126:131	arg1	soil					108:111	soil	108:111	soil of Cholistan Desert	108:131	nov., two novel actinobacteria isolated from soil of Cholistan Desert and emended description of the genus Motilibacter.
33099259	2	49	theme	Desert	126:131	arg1	description					145:155	emended description	137:155	emended description of the genus Motilibacter	137:181	nov., two novel actinobacteria isolated from soil of Cholistan Desert and emended description of the genus Motilibacter.
33099259	8	50	from	species	1545:1551	arg1	differentiation					1510:1524	the differentiation	1506:1524	the differentiation from their closest species	1506:1551	The two isolates were found to have chemotaxonomic, cultural and morphological properties consistent with their classification in the genus Motilibacter and also confirmed the differentiation from their closest species.
33099259	10	51	theme	JCM	1742:1744	arg1	nov					1716:1718	nov	1716:1718	nov	1716:1718	nov. (type strain E257T = JCM 33651T = CGMCC 1.17159T) and Motilibacter aurantiacus sp.
33099259	10	51	theme	JCM	1742:1744	arg1	1.17159T					1761:1768	type strain E257T = JCM 33651T = CGMCC 1.17159T	1722:1768	type strain E257T = JCM 33651T = CGMCC 1.17159T	1722:1768	nov. (type strain E257T = JCM 33651T = CGMCC 1.17159T) and Motilibacter aurantiacus sp.
33099259	9	52	theme	Motilibacter	1658:1669	arg1	species					1637:1643	two novel species	1627:1643	two novel species	1627:1643	The obtained results demonstrated that strains E257T and K478T represent two novel species of the genus Motilibacter, for which the names Motilibacter desertisp.
33099259	10	53	theme	E257T	1734:1738	arg1	nov					1716:1718	nov	1716:1718	nov	1716:1718	nov. (type strain E257T = JCM 33651T = CGMCC 1.17159T) and Motilibacter aurantiacus sp.
33099259	10	53	theme	E257T	1734:1738	arg1	1.17159T					1761:1768	type strain E257T = JCM 33651T = CGMCC 1.17159T	1722:1768	type strain E257T = JCM 33651T = CGMCC 1.17159T	1722:1768	nov. (type strain E257T = JCM 33651T = CGMCC 1.17159T) and Motilibacter aurantiacus sp.
33099259	3	54	theme	hyper-arid	269:278	arg1	samples					285:291	hyper-arid soil samples	269:291	hyper-arid soil samples collected in Cholistan Desert, Pakistan	269:331	Two novel actinobacterial strains, designated as E257T and K478T, were isolated from hyper-arid soil samples collected in Cholistan Desert, Pakistan.
33099259	4	55	theme	genus	430:434	arg1	Motilibacter					436:447	the genus Motilibacter	426:447	the genus Motilibacter	426:447	Comparative analysis of 16S rRNA genes showed that strains E257T and K478T were assigned to the genus Motilibacter, being their closest relative M. rhizosphaerae RS-16T with 97.3% and 96.7% similarities, respectively.
33099259	7	56	with	species	1242:1248	arg1	values					1281:1286	values	1281:1286	values below the thresholds for species delineation	1281:1331	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	8	57	theme	morphological	1399:1411	arg1	properties					1413:1422	chemotaxonomic, cultural and morphological properties	1370:1422	chemotaxonomic, cultural and morphological properties consistent with their classification in the genus Motilibacter	1370:1485	The two isolates were found to have chemotaxonomic, cultural and morphological properties consistent with their classification in the genus Motilibacter and also confirmed the differentiation from their closest species.
33099259	7	58	theme	DNA-DNA	987:993	arg1	dDDH					1010:1013	dDDH	1010:1013	dDDH	1010:1013	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	7	58	theme	DNA-DNA	987:993	arg1	hybridization					995:1007	digital DNA-DNA hybridization	979:1007	digital DNA-DNA hybridization (dDDH) values	979:1021	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	10	59	theme	=	1753:1753	arg1	nov					1716:1718	nov	1716:1718	nov	1716:1718	nov. (type strain E257T = JCM 33651T = CGMCC 1.17159T) and Motilibacter aurantiacus sp.
33099259	10	59	theme	=	1753:1753	arg1	1.17159T					1761:1768	type strain E257T = JCM 33651T = CGMCC 1.17159T	1722:1768	type strain E257T = JCM 33651T = CGMCC 1.17159T	1722:1768	nov. (type strain E257T = JCM 33651T = CGMCC 1.17159T) and Motilibacter aurantiacus sp.
33099259	8	60	theme	consistent	1424:1433	arg1	properties					1413:1422	chemotaxonomic, cultural and morphological properties	1370:1422	chemotaxonomic, cultural and morphological properties consistent with their classification in the genus Motilibacter	1370:1485	The two isolates were found to have chemotaxonomic, cultural and morphological properties consistent with their classification in the genus Motilibacter and also confirmed the differentiation from their closest species.
33099259	7	61	theme	species	1313:1319	arg1	delineation					1321:1331	species delineation	1313:1331	species delineation	1313:1331	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	10	62	theme	aurantiacus	1788:1798	arg1	sp					1800:1801	aurantiacus sp	1788:1801	aurantiacus sp	1788:1801	nov. (type strain E257T = JCM 33651T = CGMCC 1.17159T) and Motilibacter aurantiacus sp.
33099259	6	63	theme	Phylogenetic	618:629	arg1	analysis					631:638	Phylogenetic analysis	618:638	Phylogenetic analysis based on 16S rRNA gene sequences	618:671	Phylogenetic analysis based on 16S rRNA gene sequences and phylogenomic analysis based on multiple genes of conserved core proteins exhibited that these two strains belonged to the genus Motilibacter and formed a robust cluster separated from the two type species of the genus Motilibacter.
33099259	7	64	theme	genus	1166:1170	arg1	Motilibacter					1172:1183	genus Motilibacter	1166:1183	genus Motilibacter	1166:1183	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	7	65	theme	acid	958:961	arg1	AAI					973:975	AAI	973:975	AAI	973:975	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	7	65	theme	acid	958:961	arg1	Identity					963:970	Average Amino acid Identity	944:970	Average Amino acid Identity (AAI)	944:976	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	4	66	theme	rhizosphaerae	482:494	arg1	RS-16T					496:501	their closest relative M. rhizosphaerae RS-16T	456:501	their closest relative M. rhizosphaerae RS-16T	456:501	Comparative analysis of 16S rRNA genes showed that strains E257T and K478T were assigned to the genus Motilibacter, being their closest relative M. rhizosphaerae RS-16T with 97.3% and 96.7% similarities, respectively.
33099259	7	67	theme	Average	944:950	arg1	AAI					973:975	AAI	973:975	AAI	973:975	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	7	67	theme	Average	944:950	arg1	Identity					963:970	Average Amino acid Identity	944:970	Average Amino acid Identity (AAI)	944:976	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	8	68	theme	genus	1468:1472	arg1	Motilibacter					1474:1485	the genus Motilibacter	1464:1485	the genus Motilibacter	1464:1485	The two isolates were found to have chemotaxonomic, cultural and morphological properties consistent with their classification in the genus Motilibacter and also confirmed the differentiation from their closest species.
33099259	4	69	dep	strains	385:391	arg1	strains					385:391	strains	385:391	strains E257T and K478T	385:407	Comparative analysis of 16S rRNA genes showed that strains E257T and K478T were assigned to the genus Motilibacter, being their closest relative M. rhizosphaerae RS-16T with 97.3% and 96.7% similarities, respectively.
33099259	4	69	dep	strains	385:391	arg1	K478T					403:407	K478T	403:407	K478T	403:407	Comparative analysis of 16S rRNA genes showed that strains E257T and K478T were assigned to the genus Motilibacter, being their closest relative M. rhizosphaerae RS-16T with 97.3% and 96.7% similarities, respectively.
33099259	4	69	dep	strains	385:391	arg1	E257T					393:397	E257T	393:397	E257T	393:397	Comparative analysis of 16S rRNA genes showed that strains E257T and K478T were assigned to the genus Motilibacter, being their closest relative M. rhizosphaerae RS-16T with 97.3% and 96.7% similarities, respectively.
33099259	8	70	theme	cultural	1386:1393	arg1	properties					1413:1422	chemotaxonomic, cultural and morphological properties	1370:1422	chemotaxonomic, cultural and morphological properties consistent with their classification in the genus Motilibacter	1370:1485	The two isolates were found to have chemotaxonomic, cultural and morphological properties consistent with their classification in the genus Motilibacter and also confirmed the differentiation from their closest species.
33099259	5	71	theme	strain	584:589	arg1	E257T					591:595	strain E257T	584:595	strain E257T	584:595	The sequence similarity between strain E257T and K478T was 98.9%.
33099259	7	72	dep	strains	1123:1129	arg1	K478T					1141:1145	K478T	1141:1145	K478T	1141:1145	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	7	72	dep	strains	1123:1129	arg1	E257T					1131:1135	E257T	1131:1135	E257T	1131:1135	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	7	72	dep	strains	1123:1129	arg1	strains					1123:1129	strains	1123:1129	strains E257T and K478T	1123:1145	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	7	73	theme	Motilibacter	1263:1274	arg1	species					1242:1248	the other species	1232:1248	the other species of the genus Motilibacter	1232:1274	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	4	74	theme	relative	470:477	arg1	RS-16T					496:501	their closest relative M. rhizosphaerae RS-16T	456:501	their closest relative M. rhizosphaerae RS-16T	456:501	Comparative analysis of 16S rRNA genes showed that strains E257T and K478T were assigned to the genus Motilibacter, being their closest relative M. rhizosphaerae RS-16T with 97.3% and 96.7% similarities, respectively.
33099259	3	75	theme	novel	188:192	arg1	strains					210:216	Two novel actinobacterial strains	184:216	Two novel actinobacterial strains	184:216	Two novel actinobacterial strains, designated as E257T and K478T, were isolated from hyper-arid soil samples collected in Cholistan Desert, Pakistan.
33099259	0	76	theme	deserti	13:19	arg1	sp					21:22	Motilibacter deserti sp	0:22	Motilibacter deserti sp.	0:23	Motilibacter deserti sp.
33099259	4	77	dep	%	522:522	arg1	similarities					524:535	similarities	524:535	96.7% similarities	518:535	Comparative analysis of 16S rRNA genes showed that strains E257T and K478T were assigned to the genus Motilibacter, being their closest relative M. rhizosphaerae RS-16T with 97.3% and 96.7% similarities, respectively.
33099259	7	78	theme	Nucleotide	917:926	arg1	ANI					938:940	ANI	938:940	ANI	938:940	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	7	78	theme	Nucleotide	917:926	arg1	Identity					928:935	Average Nucleotide Identity	909:935	Average Nucleotide Identity (ANI)	909:941	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	6	79	theme	gene	658:661	arg1	sequences					663:671	16S rRNA gene sequences	649:671	16S rRNA gene sequences	649:671	Phylogenetic analysis based on 16S rRNA gene sequences and phylogenomic analysis based on multiple genes of conserved core proteins exhibited that these two strains belonged to the genus Motilibacter and formed a robust cluster separated from the two type species of the genus Motilibacter.
33099259	11	80	theme	33652T	1833:1838	arg1	nov					1804:1806	nov	1804:1806	nov	1804:1806	nov. (type strain K478T =JCM 33652T =CGMCC 1.17229T) are proposed.
33099259	11	80	theme	33652T	1833:1838	arg1	1.17229T					1847:1854	type strain K478T =JCM 33652T =CGMCC 1.17229T	1810:1854	type strain K478T =JCM 33652T =CGMCC 1.17229T	1810:1854	nov. (type strain K478T =JCM 33652T =CGMCC 1.17229T) are proposed.
33099259	7	81	theme	genome	1096:1101	arg1	sequences					1103:1111	the complete genome sequences	1083:1111	the complete genome sequences	1083:1111	Average Nucleotide Identity (ANI), Average Amino acid Identity (AAI), digital DNA-DNA hybridization (dDDH) values and Percentage of Conserved Proteins (POCP) calculated from the complete genome sequences indicated strains E257T and K478T were assigned into genus Motilibacter but clearly separated from each other and from the other species of the genus Motilibacter with values below the thresholds for species delineation.
33099259	2	82	theme	Motilibacter	170:181	arg1	soil					108:111	soil	108:111	soil of Cholistan Desert	108:131	nov., two novel actinobacteria isolated from soil of Cholistan Desert and emended description of the genus Motilibacter.
33099259	2	82	theme	Motilibacter	170:181	arg1	description					145:155	emended description	137:155	emended description of the genus Motilibacter	137:181	nov., two novel actinobacteria isolated from soil of Cholistan Desert and emended description of the genus Motilibacter.
33099259	6	83	theme	16S	649:651	arg1	sequences					663:671	16S rRNA gene sequences	649:671	16S rRNA gene sequences	649:671	Phylogenetic analysis based on 16S rRNA gene sequences and phylogenomic analysis based on multiple genes of conserved core proteins exhibited that these two strains belonged to the genus Motilibacter and formed a robust cluster separated from the two type species of the genus Motilibacter.
33099259	9	84	theme	obtained	1558:1565	arg1	results					1567:1573	The obtained results	1554:1573	The obtained results	1554:1573	The obtained results demonstrated that strains E257T and K478T represent two novel species of the genus Motilibacter, for which the names Motilibacter desertisp.
33099259	6	85	theme	proteins	741:748	arg1	genes					717:721	multiple genes	708:721	multiple genes of conserved core proteins	708:748	Phylogenetic analysis based on 16S rRNA gene sequences and phylogenomic analysis based on multiple genes of conserved core proteins exhibited that these two strains belonged to the genus Motilibacter and formed a robust cluster separated from the two type species of the genus Motilibacter.
33099259	3	86	attach	isolated	255:262	arg2	strains					210:216	Two novel actinobacterial strains	184:216	Two novel actinobacterial strains	184:216	Two novel actinobacterial strains, designated as E257T and K478T, were isolated from hyper-arid soil samples collected in Cholistan Desert, Pakistan.
33099259	3	86	attach	isolated	255:262	arg1	samples					285:291	hyper-arid soil samples	269:291	hyper-arid soil samples collected in Cholistan Desert, Pakistan	269:331	Two novel actinobacterial strains, designated as E257T and K478T, were isolated from hyper-arid soil samples collected in Cholistan Desert, Pakistan.
33099259	6	87	theme	conserved	726:734	arg1	proteins					741:748	conserved core proteins	726:748	conserved core proteins	726:748	Phylogenetic analysis based on 16S rRNA gene sequences and phylogenomic analysis based on multiple genes of conserved core proteins exhibited that these two strains belonged to the genus Motilibacter and formed a robust cluster separated from the two type species of the genus Motilibacter.
33099259	4	88	with	RS-16T	496:501	arg1	%					512:512	97.3%	508:512	97.3%	508:512	Comparative analysis of 16S rRNA genes showed that strains E257T and K478T were assigned to the genus Motilibacter, being their closest relative M. rhizosphaerae RS-16T with 97.3% and 96.7% similarities, respectively.
33099259	4	88	with	RS-16T	496:501	arg1	%					522:522	96.7%	518:522	96.7% similarities	518:535	Comparative analysis of 16S rRNA genes showed that strains E257T and K478T were assigned to the genus Motilibacter, being their closest relative M. rhizosphaerae RS-16T with 97.3% and 96.7% similarities, respectively.
33099259	1	89	theme	nov.	25:28	arg1	aurantiacus					47:57	nov. and Motilibacter aurantiacus	25:57	nov. and Motilibacter aurantiacus	25:57	nov. and Motilibacter aurantiacus sp.
33099259	6	90	theme	genus	799:803	arg1	Motilibacter					805:816	the genus Motilibacter	795:816	the genus Motilibacter	795:816	Phylogenetic analysis based on 16S rRNA gene sequences and phylogenomic analysis based on multiple genes of conserved core proteins exhibited that these two strains belonged to the genus Motilibacter and formed a robust cluster separated from the two type species of the genus Motilibacter.
33099259	1	91	theme	Motilibacter	34:45	arg1	aurantiacus					47:57	nov. and Motilibacter aurantiacus	25:57	nov. and Motilibacter aurantiacus	25:57	nov. and Motilibacter aurantiacus sp.
33099259	2	92	theme	Cholistan	116:124	arg1	Desert					126:131	Cholistan Desert	116:131	Cholistan Desert	116:131	nov., two novel actinobacteria isolated from soil of Cholistan Desert and emended description of the genus Motilibacter.
33099259	11	93	theme	K478T	1822:1826	arg1	nov					1804:1806	nov	1804:1806	nov	1804:1806	nov. (type strain K478T =JCM 33652T =CGMCC 1.17229T) are proposed.
33099259	11	93	theme	K478T	1822:1826	arg1	1.17229T					1847:1854	type strain K478T =JCM 33652T =CGMCC 1.17229T	1810:1854	type strain K478T =JCM 33652T =CGMCC 1.17229T	1810:1854	nov. (type strain K478T =JCM 33652T =CGMCC 1.17229T) are proposed.
32315306	9	0	theme	breast	1609:1614	arg1	IgA					1639:1641	IgA	1639:1641	IgA	1639:1641	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	9	0	theme	breast	1609:1614	arg1	A					1636:1636	breast milk immunoglobulin A	1609:1636	breast milk immunoglobulin A (IgA)	1609:1642	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	9	1	theme	immunoglobulin	1621:1634	arg1	IgA					1639:1641	IgA	1639:1641	IgA	1639:1641	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	9	1	theme	immunoglobulin	1621:1634	arg1	A					1636:1636	breast milk immunoglobulin A	1609:1636	breast milk immunoglobulin A (IgA)	1609:1642	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	8	2	theme	allergy	1356:1362	arg1	risk					1348:1351	the risk	1344:1351	the risk of allergy in the infants	1344:1377	No separation of the data based on the risk of allergy in the infants was identified using PCA.
32315306	10	3	theme	HMO	1860:1862	arg1	group					1864:1868	the HMO group	1856:1868	the HMO group	1856:1868	Graphical discovery analysis also exemplifies a possible competition for the fucosyl group between 2'FL, LNFP-I and 3'FL in the HMO group.
32315306	3	4	theme	component	568:576	arg1	interactions					586:597	breast milk component complex interactions	556:597	breast milk component complex interactions in the context of the allergy status of the mother or the child	556:661	AIM Here we aimed at visualizing breast milk component complex interactions in the context of the allergy status of the mother or the child.
32315306	11	5	theme	specific	1931:1938	arg1	group					1960:1964	blood group	1954:1964	blood group	1954:1964	Additionally, dependencies between immune component IgA and specific HMO (6'SL and blood group A antigen tetraose type 5 or PI-HMO) were identified.
32315306	11	5	theme	specific	1931:1938	arg1	HMO					1940:1942	specific HMO	1931:1942	specific HMO (6'SL and blood group A antigen tetraose type 5 or PI-HMO)	1931:2001	Additionally, dependencies between immune component IgA and specific HMO (6'SL and blood group A antigen tetraose type 5 or PI-HMO) were identified.
32315306	11	5	theme	specific	1931:1938	arg1	PI-HMO					1995:2000	PI-HMO	1995:2000	PI-HMO	1995:2000	Additionally, dependencies between immune component IgA and specific HMO (6'SL and blood group A antigen tetraose type 5 or PI-HMO) were identified.
32315306	11	5	theme	specific	1931:1938	arg1	SL					1947:1948	SL	1947:1948	SL	1947:1948	Additionally, dependencies between immune component IgA and specific HMO (6'SL and blood group A antigen tetraose type 5 or PI-HMO) were identified.
32315306	9	6	theme	graphical	1410:1418	arg1	analysis					1430:1437	graphical discovery analysis	1410:1437	graphical discovery analysis	1410:1437	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	5	7	theme	mother/infant	917:929	arg1	pairs					931:935	mother/infant pairs	917:935	mother/infant pairs	917:935	A total of 156 breast milk samples, collected at 3 months after birth from mother/infant pairs, were analyzed for 51 breast milk components.
32315306	4	8	theme	Milk	672:675	arg1	samples					677:683	METHODS Milk samples	664:683	METHODS Milk samples	664:683	METHODS Milk samples were collected from lactating mothers participating in the Leipziger Forschungszentrum für Zivilisationskrankheiten (LIFE) Child cohort in Leipzig, Germany.
32315306	1	9	from	status	302:307	arg1	mother					316:321	mother	316:321	mother	316:321	BACKGROUND Numerous studies have shown that specific components of breast milk, considered separately, are associated with disease status in the mother or the child using univariate analyses.
32315306	1	9	from	status	302:307	arg1	child					330:334	the child	326:334	the child	326:334	BACKGROUND Numerous studies have shown that specific components of breast milk, considered separately, are associated with disease status in the mother or the child using univariate analyses.
32315306	3	10	theme	breast	556:561	arg1	interactions					586:597	breast milk component complex interactions	556:597	breast milk component complex interactions in the context of the allergy status of the mother or the child	556:661	AIM Here we aimed at visualizing breast milk component complex interactions in the context of the allergy status of the mother or the child.
32315306	1	11	theme	Numerous	182:189	arg1	studies					191:197	BACKGROUND Numerous studies	171:197	BACKGROUND Numerous studies	171:197	BACKGROUND Numerous studies have shown that specific components of breast milk, considered separately, are associated with disease status in the mother or the child using univariate analyses.
32315306	13	12	theme	diseases	2353:2360	arg1	context					2331:2337	the context	2327:2337	the context of health and diseases such as allergy	2327:2376	This approach can lead to novel discoveries in the context of health and diseases such as allergy.
32315306	12	13	theme	Graphical	2031:2039	arg1	analysis					2051:2058	Graphical discovery analysis	2031:2058	CONCLUSION Graphical discovery analysis applied to complex matrices such as breast milk composition	2020:2118	CONCLUSION Graphical discovery analysis applied to complex matrices such as breast milk composition can aid in understanding the complexity of interactions between breast milk components and possible relations to health parameters in the mother or the infant.
32315306	12	14	theme	breast	2096:2101	arg1	composition					2108:2118	breast milk composition	2096:2118	breast milk composition	2096:2118	CONCLUSION Graphical discovery analysis applied to complex matrices such as breast milk composition can aid in understanding the complexity of interactions between breast milk components and possible relations to health parameters in the mother or the infant.
32315306	0	15	theme	milk	123:126	arg1	oligosaccharides					128:143	human milk oligosaccharides	117:143	human milk oligosaccharides	117:143	Novel approach to visualize the inter-dependencies between maternal sensitization, breast milk immune components and human milk oligosaccharides in the LIFE Child cohort.
32315306	5	16	theme	breast	857:862	arg1	samples					869:875	156 breast milk samples	853:875	156 breast milk samples	853:875	A total of 156 breast milk samples, collected at 3 months after birth from mother/infant pairs, were analyzed for 51 breast milk components.
32315306	3	17	theme	allergy	621:627	arg1	status					629:634	the allergy status	617:634	the allergy status of the mother or the child	617:661	AIM Here we aimed at visualizing breast milk component complex interactions in the context of the allergy status of the mother or the child.
32315306	5	18	theme	samples	869:875	arg1	total					844:848	A total	842:848	A total of 156 breast milk samples, collected at 3 months after birth from mother/infant pairs,	842:936	A total of 156 breast milk samples, collected at 3 months after birth from mother/infant pairs, were analyzed for 51 breast milk components.
32315306	0	19	theme	LIFE	152:155	arg1	cohort					163:168	the LIFE Child cohort	148:168	the LIFE Child cohort	148:168	Novel approach to visualize the inter-dependencies between maternal sensitization, breast milk immune components and human milk oligosaccharides in the LIFE Child cohort.
32315306	10	20	theme	discovery	1742:1750	arg1	analysis					1752:1759	Graphical discovery analysis	1732:1759	Graphical discovery analysis	1732:1759	Graphical discovery analysis also exemplifies a possible competition for the fucosyl group between 2'FL, LNFP-I and 3'FL in the HMO group.
32315306	9	21	theme	immunoglobulin	1486:1499	arg1	E					1501:1501	maternal plasma immunoglobulin E	1470:1501	maternal plasma immunoglobulin E (IgE) level	1470:1513	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	9	21	theme	immunoglobulin	1486:1499	arg1	IgE					1504:1506	IgE	1504:1506	IgE	1504:1506	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	11	22	theme	antigen	1968:1974	arg1	type					1985:1988	A antigen tetraose type 5	1966:1990	A antigen tetraose type 5	1966:1990	Additionally, dependencies between immune component IgA and specific HMO (6'SL and blood group A antigen tetraose type 5 or PI-HMO) were identified.
32315306	12	23	theme	possible	2211:2218	arg1	relations					2220:2228	possible relations	2211:2228	possible relations to health parameters in the mother or the infant	2211:2277	CONCLUSION Graphical discovery analysis applied to complex matrices such as breast milk composition can aid in understanding the complexity of interactions between breast milk components and possible relations to health parameters in the mother or the infant.
32315306	4	24	theme	Leipziger	744:752	arg1	cohort					814:819	the Leipziger Forschungszentrum für Zivilisationskrankheiten (LIFE) Child cohort	740:819	the Leipziger Forschungszentrum für Zivilisationskrankheiten (LIFE) Child cohort in Leipzig, Germany	740:839	METHODS Milk samples were collected from lactating mothers participating in the Leipziger Forschungszentrum für Zivilisationskrankheiten (LIFE) Child cohort in Leipzig, Germany.
32315306	3	25	from	interactions	586:597	arg1	context					606:612	the context	602:612	the context of the allergy status of the mother or the child	602:661	AIM Here we aimed at visualizing breast milk component complex interactions in the context of the allergy status of the mother or the child.
32315306	3	26	theme	mother	643:648	arg1	status					629:634	the allergy status	617:634	the allergy status of the mother or the child	617:661	AIM Here we aimed at visualizing breast milk component complex interactions in the context of the allergy status of the mother or the child.
32315306	14	27	theme	milk	2486:2489	arg1	components					2491:2500	breast milk components	2479:2500	breast milk components	2479:2500	Our study thus represents the first attempt to visualize the complexity and the inter-dependency of breast milk components.
32315306	0	28	theme	sensitization	68:80	arg1	components					102:111	maternal sensitization, breast milk immune components	59:111	components	102:111	Novel approach to visualize the inter-dependencies between maternal sensitization, breast milk immune components and human milk oligosaccharides in the LIFE Child cohort.
32315306	12	29	from	parameters	2240:2249	arg1	infant					2272:2277	the infant	2268:2277	the infant	2268:2277	CONCLUSION Graphical discovery analysis applied to complex matrices such as breast milk composition can aid in understanding the complexity of interactions between breast milk components and possible relations to health parameters in the mother or the infant.
32315306	12	29	from	parameters	2240:2249	arg1	mother					2258:2263	the mother	2254:2263	the mother	2254:2263	CONCLUSION Graphical discovery analysis applied to complex matrices such as breast milk composition can aid in understanding the complexity of interactions between breast milk components and possible relations to health parameters in the mother or the infant.
32315306	9	30	theme	birth	1704:1708	arg1	weight					1710:1715	birth weight	1704:1715	birth weight	1704:1715	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	12	31	theme	breast	2184:2189	arg1	components					2196:2205	breast milk components	2184:2205	breast milk components	2184:2205	CONCLUSION Graphical discovery analysis applied to complex matrices such as breast milk composition can aid in understanding the complexity of interactions between breast milk components and possible relations to health parameters in the mother or the infant.
32315306	11	32	theme	tetraose	1976:1983	arg1	type					1985:1988	A antigen tetraose type 5	1966:1990	A antigen tetraose type 5	1966:1990	Additionally, dependencies between immune component IgA and specific HMO (6'SL and blood group A antigen tetraose type 5 or PI-HMO) were identified.
32315306	8	33	theme	data	1330:1333	arg1	separation					1312:1321	No separation	1309:1321	No separation of the data based on the risk of allergy in the infants	1309:1377	No separation of the data based on the risk of allergy in the infants was identified using PCA.
32315306	4	34	from	cohort	814:819	arg1	Germany					833:839	Germany	833:839	Germany	833:839	METHODS Milk samples were collected from lactating mothers participating in the Leipziger Forschungszentrum für Zivilisationskrankheiten (LIFE) Child cohort in Leipzig, Germany.
32315306	11	35	dep	SL	1947:1948	arg1	type					1985:1988	A antigen tetraose type 5	1966:1990	A antigen tetraose type 5	1966:1990	Additionally, dependencies between immune component IgA and specific HMO (6'SL and blood group A antigen tetraose type 5 or PI-HMO) were identified.
32315306	6	36	theme	component	1006:1014	arg1	PCA					1026:1028	PCA	1026:1028	PCA	1026:1028	Correlation, principal component analysis (PCA) and graphical discovery analysis were used.
32315306	6	36	theme	component	1006:1014	arg1	analysis					1016:1023	principal component analysis	996:1023	principal component analysis (PCA)	996:1029	Correlation, principal component analysis (PCA) and graphical discovery analysis were used.
32315306	11	37	theme	component	1913:1921	arg1	IgA					1923:1925	immune component IgA	1906:1925	immune component IgA	1906:1925	Additionally, dependencies between immune component IgA and specific HMO (6'SL and blood group A antigen tetraose type 5 or PI-HMO) were identified.
32315306	0	38	theme	immune	95:100	arg1	components					102:111	maternal sensitization, breast milk immune components	59:111	components	102:111	Novel approach to visualize the inter-dependencies between maternal sensitization, breast milk immune components and human milk oligosaccharides in the LIFE Child cohort.
32315306	9	39	theme	breast	1523:1528	arg1	TGF-ß2					1584:1589	TGF-ß2	1584:1589	TGF-ß2	1584:1589	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	9	39	theme	breast	1523:1528	arg1	factor-beta					1569:1579	the breast milk immune marker transforming growth factor-beta 2	1519:1581	the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2)	1519:1590	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	6	40	used	used	1069:1072	arg2	Correlation					983:993	Correlation	983:993	Correlation	983:993	Correlation, principal component analysis (PCA) and graphical discovery analysis were used.
32315306	6	40	used	used	1069:1072	arg2	PCA					1026:1028	PCA	1026:1028	PCA	1026:1028	Correlation, principal component analysis (PCA) and graphical discovery analysis were used.
32315306	6	40	used	used	1069:1072	arg2	analysis					1016:1023	principal component analysis	996:1023	principal component analysis (PCA)	996:1029	Correlation, principal component analysis (PCA) and graphical discovery analysis were used.
32315306	6	40	used	used	1069:1072	arg2	analysis					1055:1062	graphical discovery analysis	1035:1062	graphical discovery analysis	1035:1062	Correlation, principal component analysis (PCA) and graphical discovery analysis were used.
32315306	9	41	theme	immune	1535:1540	arg1	TGF-ß2					1584:1589	TGF-ß2	1584:1589	TGF-ß2	1584:1589	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	9	41	theme	immune	1535:1540	arg1	factor-beta					1569:1579	the breast milk immune marker transforming growth factor-beta 2	1519:1581	the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2)	1519:1590	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	12	42	theme	interactions	2163:2174	arg1	complexity					2149:2158	the complexity	2145:2158	the complexity of interactions between breast milk components and possible relations to health parameters in the mother or the infant	2145:2277	CONCLUSION Graphical discovery analysis applied to complex matrices such as breast milk composition can aid in understanding the complexity of interactions between breast milk components and possible relations to health parameters in the mother or the infant.
32315306	4	43	theme	für	772:774	arg1	cohort					814:819	the Leipziger Forschungszentrum für Zivilisationskrankheiten (LIFE) Child cohort	740:819	the Leipziger Forschungszentrum für Zivilisationskrankheiten (LIFE) Child cohort in Leipzig, Germany	740:839	METHODS Milk samples were collected from lactating mothers participating in the Leipziger Forschungszentrum für Zivilisationskrankheiten (LIFE) Child cohort in Leipzig, Germany.
32315306	2	44	theme	few	377:379	arg1	studies					381:387	very few studies	372:387	very few studies	372:387	However, very few studies have considered multivariate analysis approaches to evaluate the relationship between multiple breast milk components simultaneously.
32315306	0	45	theme	Novel	0:4	arg1	approach					6:13	Novel approach	0:13	Novel approach	0:13	Novel approach to visualize the inter-dependencies between maternal sensitization, breast milk immune components and human milk oligosaccharides in the LIFE Child cohort.
32315306	9	46	theme	transforming	1549:1560	arg1	TGF-ß2					1584:1589	TGF-ß2	1584:1589	TGF-ß2	1584:1589	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	9	46	theme	transforming	1549:1560	arg1	factor-beta					1569:1579	the breast milk immune marker transforming growth factor-beta 2	1519:1581	the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2)	1519:1590	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	9	47	used	used	1443:1446	arg2	analysis					1430:1437	graphical discovery analysis	1410:1437	graphical discovery analysis	1410:1437	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	10	48	theme	possible	1780:1787	arg1	competition					1789:1799	a possible competition	1778:1799	a possible competition for the fucosyl group between 2'FL, LNFP-I and 3'FL	1778:1851	Graphical discovery analysis also exemplifies a possible competition for the fucosyl group between 2'FL, LNFP-I and 3'FL in the HMO group.
32315306	7	49	theme	breast	1170:1175	arg1	phospholipids					1182:1194	breast milk phospholipids	1170:1194	breast milk phospholipids	1170:1194	RESULT Correlations ranging from 0.40 to 0.96 were observed between breast milk fatty acid and breast milk phospholipids levels and correlations ranging from 0 to 0.76 between specific human milk oligosaccharides (HMO) were observed.
32315306	1	50	theme	disease	294:300	arg1	status					302:307	disease status	294:307	disease status in the mother or the child using univariate analyses	294:360	BACKGROUND Numerous studies have shown that specific components of breast milk, considered separately, are associated with disease status in the mother or the child using univariate analyses.
32315306	5	51	theme	breast	959:964	arg1	components					971:980	51 breast milk components	956:980	51 breast milk components	956:980	A total of 156 breast milk samples, collected at 3 months after birth from mother/infant pairs, were analyzed for 51 breast milk components.
32315306	2	52	theme	milk	491:494	arg1	components					496:505	multiple breast milk components	475:505	multiple breast milk components	475:505	However, very few studies have considered multivariate analysis approaches to evaluate the relationship between multiple breast milk components simultaneously.
32315306	2	53	theme	multivariate	405:416	arg1	approaches					427:436	multivariate analysis approaches	405:436	multivariate analysis approaches	405:436	However, very few studies have considered multivariate analysis approaches to evaluate the relationship between multiple breast milk components simultaneously.
32315306	9	54	theme	discovery	1420:1428	arg1	analysis					1430:1437	graphical discovery analysis	1410:1437	graphical discovery analysis	1410:1437	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	6	55	theme	graphical	1035:1043	arg1	analysis					1055:1062	graphical discovery analysis	1035:1062	graphical discovery analysis	1035:1062	Correlation, principal component analysis (PCA) and graphical discovery analysis were used.
32315306	2	56	theme	multiple	475:482	arg1	components					496:505	multiple breast milk components	475:505	multiple breast milk components	475:505	However, very few studies have considered multivariate analysis approaches to evaluate the relationship between multiple breast milk components simultaneously.
32315306	14	57	theme	first	2409:2413	arg1	attempt					2415:2421	the first attempt	2405:2421	the first attempt to visualize the complexity and the inter-dependency of breast milk components	2405:2500	Our study thus represents the first attempt to visualize the complexity and the inter-dependency of breast milk components.
32315306	12	58	dep	CONCLUSION	2020:2029	arg1	analysis					2051:2058	Graphical discovery analysis	2031:2058	CONCLUSION Graphical discovery analysis applied to complex matrices such as breast milk composition	2020:2118	CONCLUSION Graphical discovery analysis applied to complex matrices such as breast milk composition can aid in understanding the complexity of interactions between breast milk components and possible relations to health parameters in the mother or the infant.
32315306	10	59	dep	2	1831:1831	arg1	3					1848:1848	3	1848:1848	3	1848:1848	Graphical discovery analysis also exemplifies a possible competition for the fucosyl group between 2'FL, LNFP-I and 3'FL in the HMO group.
32315306	10	59	dep	2	1831:1831	arg1	LNFP-I					1837:1842	LNFP-I	1837:1842	LNFP-I	1837:1842	Graphical discovery analysis also exemplifies a possible competition for the fucosyl group between 2'FL, LNFP-I and 3'FL in the HMO group.
32315306	10	59	dep	2	1831:1831	arg1	FL					1833:1834	FL	1833:1834	FL	1833:1834	Graphical discovery analysis also exemplifies a possible competition for the fucosyl group between 2'FL, LNFP-I and 3'FL in the HMO group.
32315306	10	60	theme	2	1831:1831	arg1	FL					1850:1851	2'FL, LNFP-I and 3'FL	1831:1851	2'FL, LNFP-I and 3'FL	1831:1851	Graphical discovery analysis also exemplifies a possible competition for the fucosyl group between 2'FL, LNFP-I and 3'FL in the HMO group.
32315306	1	61	theme	milk	245:248	arg1	components					224:233	specific components	215:233	specific components	215:233	BACKGROUND Numerous studies have shown that specific components of breast milk, considered separately, are associated with disease status in the mother or the child using univariate analyses.
32315306	0	62	theme	maternal	59:66	arg1	components					102:111	maternal sensitization, breast milk immune components	59:111	components	102:111	Novel approach to visualize the inter-dependencies between maternal sensitization, breast milk immune components and human milk oligosaccharides in the LIFE Child cohort.
32315306	7	63	theme	milk	1266:1269	arg1	HMO					1289:1291	HMO	1289:1291	HMO	1289:1291	RESULT Correlations ranging from 0.40 to 0.96 were observed between breast milk fatty acid and breast milk phospholipids levels and correlations ranging from 0 to 0.76 between specific human milk oligosaccharides (HMO) were observed.
32315306	7	63	theme	milk	1266:1269	arg1	oligosaccharides					1271:1286	specific human milk oligosaccharides	1251:1286	specific human milk oligosaccharides (HMO)	1251:1292	RESULT Correlations ranging from 0.40 to 0.96 were observed between breast milk fatty acid and breast milk phospholipids levels and correlations ranging from 0 to 0.76 between specific human milk oligosaccharides (HMO) were observed.
32315306	9	64	theme	milk	1616:1619	arg1	IgA					1639:1641	IgA	1639:1641	IgA	1639:1641	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	9	64	theme	milk	1616:1619	arg1	A					1636:1636	breast milk immunoglobulin A	1609:1636	breast milk immunoglobulin A (IgA)	1609:1642	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	7	65	theme	specific	1251:1258	arg1	HMO					1289:1291	HMO	1289:1291	HMO	1289:1291	RESULT Correlations ranging from 0.40 to 0.96 were observed between breast milk fatty acid and breast milk phospholipids levels and correlations ranging from 0 to 0.76 between specific human milk oligosaccharides (HMO) were observed.
32315306	7	65	theme	specific	1251:1258	arg1	oligosaccharides					1271:1286	specific human milk oligosaccharides	1251:1286	specific human milk oligosaccharides (HMO)	1251:1292	RESULT Correlations ranging from 0.40 to 0.96 were observed between breast milk fatty acid and breast milk phospholipids levels and correlations ranging from 0 to 0.76 between specific human milk oligosaccharides (HMO) were observed.
32315306	3	66	theme	milk	563:566	arg1	interactions					586:597	breast milk component complex interactions	556:597	breast milk component complex interactions in the context of the allergy status of the mother or the child	556:661	AIM Here we aimed at visualizing breast milk component complex interactions in the context of the allergy status of the mother or the child.
32315306	1	67	theme	BACKGROUND	171:180	arg1	studies					191:197	BACKGROUND Numerous studies	171:197	BACKGROUND Numerous studies	171:197	BACKGROUND Numerous studies have shown that specific components of breast milk, considered separately, are associated with disease status in the mother or the child using univariate analyses.
32315306	4	68	theme	METHODS	664:670	arg1	samples					677:683	METHODS Milk samples	664:683	METHODS Milk samples	664:683	METHODS Milk samples were collected from lactating mothers participating in the Leipziger Forschungszentrum für Zivilisationskrankheiten (LIFE) Child cohort in Leipzig, Germany.
32315306	7	69	dep	0.96	1116:1119	arg1	to					1113:1114	to	1113:1114	to	1113:1114	RESULT Correlations ranging from 0.40 to 0.96 were observed between breast milk fatty acid and breast milk phospholipids levels and correlations ranging from 0 to 0.76 between specific human milk oligosaccharides (HMO) were observed.
32315306	10	70	theme	Graphical	1732:1740	arg1	analysis					1752:1759	Graphical discovery analysis	1732:1759	Graphical discovery analysis	1732:1759	Graphical discovery analysis also exemplifies a possible competition for the fucosyl group between 2'FL, LNFP-I and 3'FL in the HMO group.
32315306	7	71	theme	RESULT	1075:1080	arg1	Correlations					1082:1093	RESULT Correlations	1075:1093	RESULT Correlations ranging from 0.40 to 0.96	1075:1119	RESULT Correlations ranging from 0.40 to 0.96 were observed between breast milk fatty acid and breast milk phospholipids levels and correlations ranging from 0 to 0.76 between specific human milk oligosaccharides (HMO) were observed.
32315306	3	72	theme	complex	578:584	arg1	interactions					586:597	breast milk component complex interactions	556:597	breast milk component complex interactions in the context of the allergy status of the mother or the child	556:661	AIM Here we aimed at visualizing breast milk component complex interactions in the context of the allergy status of the mother or the child.
32315306	5	73	theme	156	853:855	arg1	samples					869:875	156 breast milk samples	853:875	156 breast milk samples	853:875	A total of 156 breast milk samples, collected at 3 months after birth from mother/infant pairs, were analyzed for 51 breast milk components.
32315306	5	74	theme	milk	864:867	arg1	samples					869:875	156 breast milk samples	853:875	156 breast milk samples	853:875	A total of 156 breast milk samples, collected at 3 months after birth from mother/infant pairs, were analyzed for 51 breast milk components.
32315306	11	75	theme	blood	1954:1958	arg1	group					1960:1964	blood group	1954:1964	blood group	1954:1964	Additionally, dependencies between immune component IgA and specific HMO (6'SL and blood group A antigen tetraose type 5 or PI-HMO) were identified.
32315306	11	75	theme	blood	1954:1958	arg1	HMO					1940:1942	specific HMO	1931:1942	specific HMO (6'SL and blood group A antigen tetraose type 5 or PI-HMO)	1931:2001	Additionally, dependencies between immune component IgA and specific HMO (6'SL and blood group A antigen tetraose type 5 or PI-HMO) were identified.
32315306	0	76	theme	Child	157:161	arg1	cohort					163:168	the LIFE Child cohort	148:168	the LIFE Child cohort	148:168	Novel approach to visualize the inter-dependencies between maternal sensitization, breast milk immune components and human milk oligosaccharides in the LIFE Child cohort.
32315306	9	77	theme	plasma	1479:1484	arg1	E					1501:1501	maternal plasma immunoglobulin E	1470:1501	maternal plasma immunoglobulin E (IgE) level	1470:1513	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	9	77	theme	plasma	1479:1484	arg1	IgE					1504:1506	IgE	1504:1506	IgE	1504:1506	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	9	78	theme	breast	1674:1679	arg1	milk					1681:1684	breast milk total protein and birth weight	1674:1715	milk	1681:1684	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	13	79	from	discoveries	2312:2322	arg1	context					2331:2337	the context	2327:2337	the context of health and diseases such as allergy	2327:2376	This approach can lead to novel discoveries in the context of health and diseases such as allergy.
32315306	11	80	theme	immune	1906:1911	arg1	IgA					1923:1925	immune component IgA	1906:1925	immune component IgA	1906:1925	Additionally, dependencies between immune component IgA and specific HMO (6'SL and blood group A antigen tetraose type 5 or PI-HMO) were identified.
32315306	9	81	theme	E	1501:1501	arg1	level					1509:1513	maternal plasma immunoglobulin E (IgE) level	1470:1513	maternal plasma immunoglobulin E (IgE) level	1470:1513	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	14	82	theme	components	2491:2500	arg1	complexity					2440:2449	the complexity	2436:2449	the complexity	2436:2449	Our study thus represents the first attempt to visualize the complexity and the inter-dependency of breast milk components.
32315306	14	82	theme	components	2491:2500	arg1	inter-dependency					2459:2474	the inter-dependency	2455:2474	the inter-dependency	2455:2474	Our study thus represents the first attempt to visualize the complexity and the inter-dependency of breast milk components.
32315306	9	83	theme	total	1686:1690	arg1	protein					1692:1698	total protein	1686:1698	total protein	1686:1698	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	12	84	theme	health	2233:2238	arg1	parameters					2240:2249	health parameters	2233:2249	health parameters in the mother or the infant	2233:2277	CONCLUSION Graphical discovery analysis applied to complex matrices such as breast milk composition can aid in understanding the complexity of interactions between breast milk components and possible relations to health parameters in the mother or the infant.
32315306	3	85	theme	status	629:634	arg1	context					606:612	the context	602:612	the context of the allergy status of the mother or the child	602:661	AIM Here we aimed at visualizing breast milk component complex interactions in the context of the allergy status of the mother or the child.
32315306	14	86	theme	breast	2479:2484	arg1	components					2491:2500	breast milk components	2479:2500	breast milk components	2479:2500	Our study thus represents the first attempt to visualize the complexity and the inter-dependency of breast milk components.
32315306	12	87	theme	milk	2191:2194	arg1	components					2196:2205	breast milk components	2184:2205	breast milk components	2184:2205	CONCLUSION Graphical discovery analysis applied to complex matrices such as breast milk composition can aid in understanding the complexity of interactions between breast milk components and possible relations to health parameters in the mother or the infant.
32315306	0	88	theme	milk	90:93	arg1	components					102:111	maternal sensitization, breast milk immune components	59:111	components	102:111	Novel approach to visualize the inter-dependencies between maternal sensitization, breast milk immune components and human milk oligosaccharides in the LIFE Child cohort.
32315306	13	89	theme	novel	2306:2310	arg1	discoveries					2312:2322	novel discoveries	2306:2322	novel discoveries in the context of health and diseases such as allergy	2306:2376	This approach can lead to novel discoveries in the context of health and diseases such as allergy.
32315306	4	90	theme	lactating	705:713	arg1	mothers					715:721	lactating mothers	705:721	lactating mothers participating in the Leipziger Forschungszentrum für Zivilisationskrankheiten (LIFE) Child cohort in Leipzig, Germany	705:839	METHODS Milk samples were collected from lactating mothers participating in the Leipziger Forschungszentrum für Zivilisationskrankheiten (LIFE) Child cohort in Leipzig, Germany.
32315306	9	91	dep	milk	1681:1684	arg1	protein					1692:1698	total protein	1686:1698	total protein	1686:1698	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	1	92	theme	univariate	342:351	arg1	analyses					353:360	univariate analyses	342:360	univariate analyses	342:360	BACKGROUND Numerous studies have shown that specific components of breast milk, considered separately, are associated with disease status in the mother or the child using univariate analyses.
32315306	5	93	theme	milk	966:969	arg1	components					971:980	51 breast milk components	956:980	51 breast milk components	956:980	A total of 156 breast milk samples, collected at 3 months after birth from mother/infant pairs, were analyzed for 51 breast milk components.
32315306	6	94	theme	principal	996:1004	arg1	PCA					1026:1028	PCA	1026:1028	PCA	1026:1028	Correlation, principal component analysis (PCA) and graphical discovery analysis were used.
32315306	6	94	theme	principal	996:1004	arg1	analysis					1016:1023	principal component analysis	996:1023	principal component analysis (PCA)	996:1029	Correlation, principal component analysis (PCA) and graphical discovery analysis were used.
32315306	1	95	dep	mother	316:321	arg1	the					312:314	the	312:314	the	312:314	BACKGROUND Numerous studies have shown that specific components of breast milk, considered separately, are associated with disease status in the mother or the child using univariate analyses.
32315306	4	96	theme	LIFE	802:805	arg1	cohort					814:819	the Leipziger Forschungszentrum für Zivilisationskrankheiten (LIFE) Child cohort	740:819	the Leipziger Forschungszentrum für Zivilisationskrankheiten (LIFE) Child cohort in Leipzig, Germany	740:839	METHODS Milk samples were collected from lactating mothers participating in the Leipziger Forschungszentrum für Zivilisationskrankheiten (LIFE) Child cohort in Leipzig, Germany.
32315306	0	97	theme	human	117:121	arg1	oligosaccharides					128:143	human milk oligosaccharides	117:143	human milk oligosaccharides	117:143	Novel approach to visualize the inter-dependencies between maternal sensitization, breast milk immune components and human milk oligosaccharides in the LIFE Child cohort.
32315306	0	98	dep	components	102:111	arg1	breast					83:88	maternal sensitization, breast milk immune components	59:111	breast	83:88	Novel approach to visualize the inter-dependencies between maternal sensitization, breast milk immune components and human milk oligosaccharides in the LIFE Child cohort.
32315306	7	99	dep	milk	1150:1153	arg1	levels					1196:1201	levels	1196:1201	levels	1196:1201	RESULT Correlations ranging from 0.40 to 0.96 were observed between breast milk fatty acid and breast milk phospholipids levels and correlations ranging from 0 to 0.76 between specific human milk oligosaccharides (HMO) were observed.
32315306	7	99	dep	milk	1150:1153	arg1	acid					1161:1164	fatty acid	1155:1164	fatty acid	1155:1164	RESULT Correlations ranging from 0.40 to 0.96 were observed between breast milk fatty acid and breast milk phospholipids levels and correlations ranging from 0 to 0.76 between specific human milk oligosaccharides (HMO) were observed.
32315306	4	100	theme	Child	808:812	arg1	cohort					814:819	the Leipziger Forschungszentrum für Zivilisationskrankheiten (LIFE) Child cohort	740:819	the Leipziger Forschungszentrum für Zivilisationskrankheiten (LIFE) Child cohort in Leipzig, Germany	740:839	METHODS Milk samples were collected from lactating mothers participating in the Leipziger Forschungszentrum für Zivilisationskrankheiten (LIFE) Child cohort in Leipzig, Germany.
32315306	7	101	theme	milk	1177:1180	arg1	phospholipids					1182:1194	breast milk phospholipids	1170:1194	breast milk phospholipids	1170:1194	RESULT Correlations ranging from 0.40 to 0.96 were observed between breast milk fatty acid and breast milk phospholipids levels and correlations ranging from 0 to 0.76 between specific human milk oligosaccharides (HMO) were observed.
32315306	9	102	theme	marker	1542:1547	arg1	TGF-ß2					1584:1589	TGF-ß2	1584:1589	TGF-ß2	1584:1589	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	9	102	theme	marker	1542:1547	arg1	factor-beta					1569:1579	the breast milk immune marker transforming growth factor-beta 2	1519:1581	the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2)	1519:1590	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	4	103	theme	Forschungszentrum	754:770	arg1	cohort					814:819	the Leipziger Forschungszentrum für Zivilisationskrankheiten (LIFE) Child cohort	740:819	the Leipziger Forschungszentrum für Zivilisationskrankheiten (LIFE) Child cohort in Leipzig, Germany	740:839	METHODS Milk samples were collected from lactating mothers participating in the Leipziger Forschungszentrum für Zivilisationskrankheiten (LIFE) Child cohort in Leipzig, Germany.
32315306	9	104	theme	growth	1562:1567	arg1	TGF-ß2					1584:1589	TGF-ß2	1584:1589	TGF-ß2	1584:1589	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	9	104	theme	growth	1562:1567	arg1	factor-beta					1569:1579	the breast milk immune marker transforming growth factor-beta 2	1519:1581	the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2)	1519:1590	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	9	105	theme	milk	1530:1533	arg1	TGF-ß2					1584:1589	TGF-ß2	1584:1589	TGF-ß2	1584:1589	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	9	105	theme	milk	1530:1533	arg1	factor-beta					1569:1579	the breast milk immune marker transforming growth factor-beta 2	1519:1581	the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2)	1519:1590	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	3	106	theme	child	657:661	arg1	status					629:634	the allergy status	617:634	the allergy status of the mother or the child	617:661	AIM Here we aimed at visualizing breast milk component complex interactions in the context of the allergy status of the mother or the child.
32315306	4	107	theme	Zivilisationskrankheiten	776:799	arg1	cohort					814:819	the Leipziger Forschungszentrum für Zivilisationskrankheiten (LIFE) Child cohort	740:819	the Leipziger Forschungszentrum für Zivilisationskrankheiten (LIFE) Child cohort in Leipzig, Germany	740:839	METHODS Milk samples were collected from lactating mothers participating in the Leipziger Forschungszentrum für Zivilisationskrankheiten (LIFE) Child cohort in Leipzig, Germany.
32315306	2	108	theme	analysis	418:425	arg1	approaches					427:436	multivariate analysis approaches	405:436	multivariate analysis approaches	405:436	However, very few studies have considered multivariate analysis approaches to evaluate the relationship between multiple breast milk components simultaneously.
32315306	7	109	theme	fatty	1155:1159	arg1	acid					1161:1164	fatty acid	1155:1164	fatty acid	1155:1164	RESULT Correlations ranging from 0.40 to 0.96 were observed between breast milk fatty acid and breast milk phospholipids levels and correlations ranging from 0 to 0.76 between specific human milk oligosaccharides (HMO) were observed.
32315306	1	110	theme	specific	215:222	arg1	components					224:233	specific components	215:233	specific components	215:233	BACKGROUND Numerous studies have shown that specific components of breast milk, considered separately, are associated with disease status in the mother or the child using univariate analyses.
32315306	6	111	theme	discovery	1045:1053	arg1	analysis					1055:1062	graphical discovery analysis	1035:1062	graphical discovery analysis	1035:1062	Correlation, principal component analysis (PCA) and graphical discovery analysis were used.
32315306	2	112	theme	breast	484:489	arg1	components					496:505	multiple breast milk components	475:505	multiple breast milk components	475:505	However, very few studies have considered multivariate analysis approaches to evaluate the relationship between multiple breast milk components simultaneously.
32315306	10	113	theme	fucosyl	1809:1815	arg1	group					1817:1821	the fucosyl group	1805:1821	the fucosyl group	1805:1821	Graphical discovery analysis also exemplifies a possible competition for the fucosyl group between 2'FL, LNFP-I and 3'FL in the HMO group.
32315306	12	114	theme	complex	2071:2077	arg1	composition					2108:2118	breast milk composition	2096:2118	breast milk composition	2096:2118	CONCLUSION Graphical discovery analysis applied to complex matrices such as breast milk composition can aid in understanding the complexity of interactions between breast milk components and possible relations to health parameters in the mother or the infant.
32315306	12	114	theme	complex	2071:2077	arg1	matrices					2079:2086	complex matrices	2071:2086	complex matrices such as breast milk composition	2071:2118	CONCLUSION Graphical discovery analysis applied to complex matrices such as breast milk composition can aid in understanding the complexity of interactions between breast milk components and possible relations to health parameters in the mother or the infant.
32315306	7	115	theme	breast	1143:1148	arg1	milk					1150:1153	breast milk fatty acid and breast milk phospholipids levels	1143:1201	milk	1150:1153	RESULT Correlations ranging from 0.40 to 0.96 were observed between breast milk fatty acid and breast milk phospholipids levels and correlations ranging from 0 to 0.76 between specific human milk oligosaccharides (HMO) were observed.
32315306	8	116	from	risk	1348:1351	arg1	infants					1371:1377	the infants	1367:1377	the infants	1367:1377	No separation of the data based on the risk of allergy in the infants was identified using PCA.
32315306	13	117	theme	health	2342:2347	arg1	context					2331:2337	the context	2327:2337	the context of health and diseases such as allergy	2327:2376	This approach can lead to novel discoveries in the context of health and diseases such as allergy.
32315306	12	118	theme	discovery	2041:2049	arg1	analysis					2051:2058	Graphical discovery analysis	2031:2058	CONCLUSION Graphical discovery analysis applied to complex matrices such as breast milk composition	2020:2118	CONCLUSION Graphical discovery analysis applied to complex matrices such as breast milk composition can aid in understanding the complexity of interactions between breast milk components and possible relations to health parameters in the mother or the infant.
32315306	1	119	theme	breast	238:243	arg1	milk					245:248	breast milk	238:248	breast milk	238:248	BACKGROUND Numerous studies have shown that specific components of breast milk, considered separately, are associated with disease status in the mother or the child using univariate analyses.
32315306	9	120	theme	maternal	1470:1477	arg1	E					1501:1501	maternal plasma immunoglobulin E	1470:1501	maternal plasma immunoglobulin E (IgE) level	1470:1513	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	9	120	theme	maternal	1470:1477	arg1	IgE					1504:1506	IgE	1504:1506	IgE	1504:1506	When graphical discovery analysis was used, dependencies between maternal plasma immunoglobulin E (IgE) level and the breast milk immune marker transforming growth factor-beta 2 (TGF-ß2), between TGF-ß2, breast milk immunoglobulin A (IgA) and TGF-ß1 as well as between breast milk total protein and birth weight were observed.
32315306	7	121	theme	human	1260:1264	arg1	HMO					1289:1291	HMO	1289:1291	HMO	1289:1291	RESULT Correlations ranging from 0.40 to 0.96 were observed between breast milk fatty acid and breast milk phospholipids levels and correlations ranging from 0 to 0.76 between specific human milk oligosaccharides (HMO) were observed.
32315306	7	121	theme	human	1260:1264	arg1	oligosaccharides					1271:1286	specific human milk oligosaccharides	1251:1286	specific human milk oligosaccharides (HMO)	1251:1292	RESULT Correlations ranging from 0.40 to 0.96 were observed between breast milk fatty acid and breast milk phospholipids levels and correlations ranging from 0 to 0.76 between specific human milk oligosaccharides (HMO) were observed.
32315306	12	122	theme	milk	2103:2106	arg1	composition					2108:2118	breast milk composition	2096:2118	breast milk composition	2096:2118	CONCLUSION Graphical discovery analysis applied to complex matrices such as breast milk composition can aid in understanding the complexity of interactions between breast milk components and possible relations to health parameters in the mother or the infant.
33909547	12	0	theme	kuwaitense	1737:1746	arg1	nov.					1753:1756	the name Litoribacterium kuwaitense gen. nov.	1712:1756	the name Litoribacterium kuwaitense gen. nov.	1712:1756	On the basis of the phenotypic, phylogenetic and genetic data, strain SIJ1T represents a novel genus and species in the family Bacillaceae, for which the name Litoribacterium kuwaitense gen. nov., sp.
33909547	2	1	theme	Gram-stain-positive	85:103	arg1	bacterium					165:173	A Gram-stain-positive, strictly aerobic, spore-forming, rod-shaped and non-motile bacterium	83:173	A Gram-stain-positive, strictly aerobic, spore-forming, rod-shaped and non-motile bacterium designated strain SIJ1T	83:197	A Gram-stain-positive, strictly aerobic, spore-forming, rod-shaped and non-motile bacterium designated strain SIJ1T was obtained from tidal flat sediment collected from the northern shore of Kuwait Bay, northwest of the Arabian Gulf.
33909547	10	2	theme	identity	1339:1346	arg1	data					1348:1351	The amino acid identity and average nucleotide identity data	1292:1351	The amino acid identity and average nucleotide identity data for the whole genome	1292:1372	The amino acid identity and average nucleotide identity data for the whole genome proved that strain SIJ1T does indeed represent a novel genus.
33909547	10	3	theme	average	1320:1326	arg1	identity					1339:1346	average nucleotide identity	1320:1346	average nucleotide identity	1320:1346	The amino acid identity and average nucleotide identity data for the whole genome proved that strain SIJ1T does indeed represent a novel genus.
33909547	8	4	theme	genome	1093:1098	arg1	size					1100:1103	a genome size	1091:1103	a genome size of 3 989 945 bp	1091:1119	The DNA G+C content was 43.1 mol% and the full genome analysis for strain SIJ1T showed that it had a genome size of 3 989 945 bp and contained 4085 predicted protein-encoding genes.
33909547	10	5	theme	whole	1361:1365	arg1	genome					1367:1372	the whole genome	1357:1372	the whole genome	1357:1372	The amino acid identity and average nucleotide identity data for the whole genome proved that strain SIJ1T does indeed represent a novel genus.
33909547	2	6	theme	northern	256:263	arg1	shore					265:269	the northern shore	252:269	the northern shore of Kuwait Bay, northwest of the Arabian Gulf	252:314	A Gram-stain-positive, strictly aerobic, spore-forming, rod-shaped and non-motile bacterium designated strain SIJ1T was obtained from tidal flat sediment collected from the northern shore of Kuwait Bay, northwest of the Arabian Gulf.
33909547	12	7	theme	family	1682:1687	arg1	Bacillaceae					1689:1699	the family Bacillaceae	1678:1699	the family Bacillaceae	1678:1699	On the basis of the phenotypic, phylogenetic and genetic data, strain SIJ1T represents a novel genus and species in the family Bacillaceae, for which the name Litoribacterium kuwaitense gen. nov., sp.
33909547	2	8	theme	Bay	281:283	arg1	shore					265:269	the northern shore	252:269	the northern shore of Kuwait Bay, northwest of the Arabian Gulf	252:314	A Gram-stain-positive, strictly aerobic, spore-forming, rod-shaped and non-motile bacterium designated strain SIJ1T was obtained from tidal flat sediment collected from the northern shore of Kuwait Bay, northwest of the Arabian Gulf.
33909547	14	9	theme	=DSM	1807:1810	arg1	SIJ1T					1800:1804	SIJ1T	1800:1804	SIJ1T (=DSM 28862T=LMG 28316T)	1800:1829	The type strain is SIJ1T (=DSM 28862T=LMG 28316T).
33909547	14	9	theme	=DSM	1807:1810	arg1	28316T					1823:1828	=DSM 28862T=LMG 28316T	1807:1828	=DSM 28862T=LMG 28316T	1807:1828	The type strain is SIJ1T (=DSM 28862T=LMG 28316T).
33909547	12	10	from	species	1667:1673	arg1	Bacillaceae					1689:1699	the family Bacillaceae	1678:1699	the family Bacillaceae	1678:1699	On the basis of the phenotypic, phylogenetic and genetic data, strain SIJ1T represents a novel genus and species in the family Bacillaceae, for which the name Litoribacterium kuwaitense gen. nov., sp.
33909547	2	11	theme	tidal	217:221	arg1	sediment					228:235	tidal flat sediment	217:235	tidal flat sediment collected from the northern shore of Kuwait Bay, northwest of the Arabian Gulf	217:314	A Gram-stain-positive, strictly aerobic, spore-forming, rod-shaped and non-motile bacterium designated strain SIJ1T was obtained from tidal flat sediment collected from the northern shore of Kuwait Bay, northwest of the Arabian Gulf.
33909547	8	12	theme	protein-encoding	1150:1165	arg1	genes					1167:1171	4085 predicted protein-encoding genes	1135:1171	4085 predicted protein-encoding genes	1135:1171	The DNA G+C content was 43.1 mol% and the full genome analysis for strain SIJ1T showed that it had a genome size of 3 989 945 bp and contained 4085 predicted protein-encoding genes.
33909547	5	13	dep	acids	648:652	arg1	the					632:634	the	632:634	the	632:634	It contained anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0 as the major fatty acids and ribose as the major whole-cell sugar.
33909547	12	14	from	genus	1657:1661	arg1	Bacillaceae					1689:1699	the family Bacillaceae	1678:1699	the family Bacillaceae	1678:1699	On the basis of the phenotypic, phylogenetic and genetic data, strain SIJ1T represents a novel genus and species in the family Bacillaceae, for which the name Litoribacterium kuwaitense gen. nov., sp.
33909547	9	15	theme	annotated	1184:1192	arg1	genome					1194:1199	The SIJ1T annotated genome	1174:1199	The SIJ1T annotated genome	1174:1199	The SIJ1T annotated genome showed more stress resistance encoding genes in comparison to its closely related strains.
33909547	7	16	theme	distinct	939:946	arg1	lineage					961:967	a distinct evolutionary lineage	937:967	a distinct evolutionary lineage	937:967	Phylogenetic analysis based on 16S rRNA genes revealed that SIJ1T showed a distinct evolutionary lineage within the Firmicutes.
33909547	11	17	theme	related	1493:1499	arg1	genera					1501:1506	the phylogenetically related genera	1472:1506	the phylogenetically related genera through differences in several phenotypic properties	1472:1559	The strain was distinguishable from the phylogenetically related genera through differences in several phenotypic properties.
33909547	14	18	theme	type	1785:1788	arg1	SIJ1T					1800:1804	SIJ1T	1800:1804	SIJ1T (=DSM 28862T=LMG 28316T)	1800:1829	The type strain is SIJ1T (=DSM 28862T=LMG 28316T).
33909547	14	18	theme	type	1785:1788	arg1	strain					1790:1795	The type strain	1781:1795	The type strain	1781:1795	The type strain is SIJ1T (=DSM 28862T=LMG 28316T).
33909547	9	19	theme	stress	1213:1218	arg1	genes					1240:1244	more stress resistance encoding genes	1208:1244	more stress resistance encoding genes	1208:1244	The SIJ1T annotated genome showed more stress resistance encoding genes in comparison to its closely related strains.
33909547	10	20	theme	strain	1386:1391	arg1	SIJ1T					1393:1397	strain SIJ1T	1386:1397	strain SIJ1T	1386:1397	The amino acid identity and average nucleotide identity data for the whole genome proved that strain SIJ1T does indeed represent a novel genus.
33909547	5	21	theme	major	672:676	arg1	sugar					689:693	the major whole-cell sugar	668:693	the major whole-cell sugar	668:693	It contained anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0 as the major fatty acids and ribose as the major whole-cell sugar.
33909547	9	22	theme	encoding	1231:1238	arg1	genes					1240:1244	more stress resistance encoding genes	1208:1244	more stress resistance encoding genes	1208:1244	The SIJ1T annotated genome showed more stress resistance encoding genes in comparison to its closely related strains.
33909547	4	23	theme	meso-diaminopimelic	441:459	arg1	acid					461:464	meso-diaminopimelic acid	441:464	meso-diaminopimelic acid	441:464	The cell-wall peptidoglycan was based on meso-diaminopimelic acid and an unsaturated menaquinone with seven isoprene units (MK-7) was the predominant respiratory quinone.
33909547	5	24	theme	fatty	642:646	arg1	acids					648:652	major fatty acids	636:652	major fatty acids	636:652	It contained anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0 as the major fatty acids and ribose as the major whole-cell sugar.
33909547	5	24	theme	fatty	642:646	arg1	anteiso-C15 					584:595	anteiso-C15 	584:595	anteiso-C15 	584:595	It contained anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0 as the major fatty acids and ribose as the major whole-cell sugar.
33909547	7	25	theme	16S	895:897	arg1	genes					904:908	16S rRNA genes	895:908	16S rRNA genes	895:908	Phylogenetic analysis based on 16S rRNA genes revealed that SIJ1T showed a distinct evolutionary lineage within the Firmicutes.
33909547	12	26	theme	genetic	1611:1617	arg1	data					1619:1622	the phenotypic, phylogenetic and genetic data	1578:1622	the phenotypic, phylogenetic and genetic data	1578:1622	On the basis of the phenotypic, phylogenetic and genetic data, strain SIJ1T represents a novel genus and species in the family Bacillaceae, for which the name Litoribacterium kuwaitense gen. nov., sp.
33909547	3	27	theme	Strain	317:322	arg1	SIJ1T					324:328	Strain SIJ1T	317:328	Strain SIJ1T	317:328	Strain SIJ1T grew optimally at 30 °C and pH 7-8 in the presence of 6 % (w/v) NaCl.
33909547	3	28	theme	%	386:386	arg1	NaCl					394:397	6 % (w/v) NaCl	384:397	6 % (w/v) NaCl	384:397	Strain SIJ1T grew optimally at 30 °C and pH 7-8 in the presence of 6 % (w/v) NaCl.
33909547	8	29	theme	DNA	996:998	arg1	content					1004:1010	The DNA G+C content	992:1010	The DNA G+C content	992:1010	The DNA G+C content was 43.1 mol% and the full genome analysis for strain SIJ1T showed that it had a genome size of 3 989 945 bp and contained 4085 predicted protein-encoding genes.
33909547	8	29	theme	DNA	996:998	arg1	%					1024:1024	43.1 mol%	1016:1024	43.1 mol%	1016:1024	The DNA G+C content was 43.1 mol% and the full genome analysis for strain SIJ1T showed that it had a genome size of 3 989 945 bp and contained 4085 predicted protein-encoding genes.
33909547	0	30	theme	Litoribacterium	0:14	arg1	nov.					32:35	Litoribacterium kuwaitense gen. nov.	0:35	Litoribacterium kuwaitense gen. nov.	0:35	Litoribacterium kuwaitense gen. nov., sp.
33909547	7	31	theme	Phylogenetic	864:875	arg1	analysis					877:884	Phylogenetic analysis	864:884	Phylogenetic analysis based on 16S rRNA genes	864:908	Phylogenetic analysis based on 16S rRNA genes revealed that SIJ1T showed a distinct evolutionary lineage within the Firmicutes.
33909547	1	32	theme	tidal	71:75	arg1	flat					77:80	a Kuwait tidal flat	62:80	a Kuwait tidal flat	62:80	nov., isolated from a Kuwait tidal flat.
33909547	12	33	theme	phenotypic	1582:1591	arg1	data					1619:1622	the phenotypic, phylogenetic and genetic data	1578:1622	the phenotypic, phylogenetic and genetic data	1578:1622	On the basis of the phenotypic, phylogenetic and genetic data, strain SIJ1T represents a novel genus and species in the family Bacillaceae, for which the name Litoribacterium kuwaitense gen. nov., sp.
33909547	12	34	dep	sp	1759:1760	arg1	nov.					1753:1756	the name Litoribacterium kuwaitense gen. nov.	1712:1756	the name Litoribacterium kuwaitense gen. nov.	1712:1756	On the basis of the phenotypic, phylogenetic and genetic data, strain SIJ1T represents a novel genus and species in the family Bacillaceae, for which the name Litoribacterium kuwaitense gen. nov., sp.
33909547	3	35	theme	NaCl	394:397	arg1	presence					372:379	the presence	368:379	the presence of 6 % (w/v) NaCl	368:397	Strain SIJ1T grew optimally at 30 °C and pH 7-8 in the presence of 6 % (w/v) NaCl.
33909547	0	36	theme	gen.	27:30	arg1	nov.					32:35	Litoribacterium kuwaitense gen. nov.	0:35	Litoribacterium kuwaitense gen. nov.	0:35	Litoribacterium kuwaitense gen. nov., sp.
33909547	8	37	theme	43.1 mol	1016:1023	arg1	content					1004:1010	The DNA G+C content	992:1010	The DNA G+C content	992:1010	The DNA G+C content was 43.1 mol% and the full genome analysis for strain SIJ1T showed that it had a genome size of 3 989 945 bp and contained 4085 predicted protein-encoding genes.
33909547	8	37	theme	43.1 mol	1016:1023	arg1	%					1024:1024	43.1 mol%	1016:1024	43.1 mol%	1016:1024	The DNA G+C content was 43.1 mol% and the full genome analysis for strain SIJ1T showed that it had a genome size of 3 989 945 bp and contained 4085 predicted protein-encoding genes.
33909547	6	38	theme	unidentified	794:805	arg1	phosphoglycolipid					819:835	phosphoglycolipid	819:835	phosphoglycolipid	819:835	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipid, an unidentified glycolipid, phosphoglycolipid and an unidentified lipid.
33909547	6	38	theme	unidentified	794:805	arg1	glycolipid					807:816	an unidentified glycolipid	791:816	an unidentified glycolipid	791:816	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipid, an unidentified glycolipid, phosphoglycolipid and an unidentified lipid.
33909547	12	39	theme	phylogenetic	1594:1605	arg1	data					1619:1622	the phenotypic, phylogenetic and genetic data	1578:1622	the phenotypic, phylogenetic and genetic data	1578:1622	On the basis of the phenotypic, phylogenetic and genetic data, strain SIJ1T represents a novel genus and species in the family Bacillaceae, for which the name Litoribacterium kuwaitense gen. nov., sp.
33909547	4	40	theme	isoprene	508:515	arg1	MK-7					524:527	MK-7	524:527	MK-7	524:527	The cell-wall peptidoglycan was based on meso-diaminopimelic acid and an unsaturated menaquinone with seven isoprene units (MK-7) was the predominant respiratory quinone.
33909547	4	40	theme	isoprene	508:515	arg1	units					517:521	seven isoprene units	502:521	seven isoprene units (MK-7)	502:528	The cell-wall peptidoglycan was based on meso-diaminopimelic acid and an unsaturated menaquinone with seven isoprene units (MK-7) was the predominant respiratory quinone.
33909547	10	41	theme	novel	1423:1427	arg1	genus					1429:1433	a novel genus	1421:1433	a novel genus	1421:1433	The amino acid identity and average nucleotide identity data for the whole genome proved that strain SIJ1T does indeed represent a novel genus.
33909547	2	42	theme	non-motile	154:163	arg1	bacterium					165:173	A Gram-stain-positive, strictly aerobic, spore-forming, rod-shaped and non-motile bacterium	83:173	A Gram-stain-positive, strictly aerobic, spore-forming, rod-shaped and non-motile bacterium designated strain SIJ1T	83:197	A Gram-stain-positive, strictly aerobic, spore-forming, rod-shaped and non-motile bacterium designated strain SIJ1T was obtained from tidal flat sediment collected from the northern shore of Kuwait Bay, northwest of the Arabian Gulf.
33909547	2	43	theme	rod-shaped	139:148	arg1	bacterium					165:173	A Gram-stain-positive, strictly aerobic, spore-forming, rod-shaped and non-motile bacterium	83:173	A Gram-stain-positive, strictly aerobic, spore-forming, rod-shaped and non-motile bacterium designated strain SIJ1T	83:197	A Gram-stain-positive, strictly aerobic, spore-forming, rod-shaped and non-motile bacterium designated strain SIJ1T was obtained from tidal flat sediment collected from the northern shore of Kuwait Bay, northwest of the Arabian Gulf.
33909547	8	44	theme	full	1034:1037	arg1	analysis					1046:1053	the full genome analysis	1030:1053	the full genome analysis for strain SIJ1T	1030:1070	The DNA G+C content was 43.1 mol% and the full genome analysis for strain SIJ1T showed that it had a genome size of 3 989 945 bp and contained 4085 predicted protein-encoding genes.
33909547	6	45	theme	unidentified	764:775	arg1	phospholipid					777:788	unidentified phospholipid	764:788	unidentified phospholipid	764:788	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipid, an unidentified glycolipid, phosphoglycolipid and an unidentified lipid.
33909547	4	46	theme	unsaturated	473:483	arg1	menaquinone					485:495	an unsaturated menaquinone	470:495	an unsaturated menaquinone with seven isoprene units (MK-7)	470:528	The cell-wall peptidoglycan was based on meso-diaminopimelic acid and an unsaturated menaquinone with seven isoprene units (MK-7) was the predominant respiratory quinone.
33909547	4	46	theme	unsaturated	473:483	arg1	quinone					562:568	the predominant respiratory quinone	534:568	the predominant respiratory quinone	534:568	The cell-wall peptidoglycan was based on meso-diaminopimelic acid and an unsaturated menaquinone with seven isoprene units (MK-7) was the predominant respiratory quinone.
33909547	11	47	theme	phenotypic	1539:1548	arg1	properties					1550:1559	several phenotypic properties	1531:1559	several phenotypic properties	1531:1559	The strain was distinguishable from the phylogenetically related genera through differences in several phenotypic properties.
33909547	2	48	theme	Arabian	303:309	arg1	Gulf					311:314	the Arabian Gulf	299:314	the Arabian Gulf	299:314	A Gram-stain-positive, strictly aerobic, spore-forming, rod-shaped and non-motile bacterium designated strain SIJ1T was obtained from tidal flat sediment collected from the northern shore of Kuwait Bay, northwest of the Arabian Gulf.
33909547	8	49	contain	had	1087:1089	arg2	size					1100:1103	a genome size	1091:1103	a genome size of 3 989 945 bp	1091:1119	The DNA G+C content was 43.1 mol% and the full genome analysis for strain SIJ1T showed that it had a genome size of 3 989 945 bp and contained 4085 predicted protein-encoding genes.
33909547	8	49	contain	had	1087:1089	arg1	it					1084:1085	it	1084:1085	it	1084:1085	The DNA G+C content was 43.1 mol% and the full genome analysis for strain SIJ1T showed that it had a genome size of 3 989 945 bp and contained 4085 predicted protein-encoding genes.
33909547	0	50	dep	sp	38:39	arg1	nov.					32:35	Litoribacterium kuwaitense gen. nov.	0:35	Litoribacterium kuwaitense gen. nov.	0:35	Litoribacterium kuwaitense gen. nov., sp.
33909547	12	51	theme	Litoribacterium	1721:1735	arg1	nov.					1753:1756	the name Litoribacterium kuwaitense gen. nov.	1712:1756	the name Litoribacterium kuwaitense gen. nov.	1712:1756	On the basis of the phenotypic, phylogenetic and genetic data, strain SIJ1T represents a novel genus and species in the family Bacillaceae, for which the name Litoribacterium kuwaitense gen. nov., sp.
33909547	10	52	theme	acid	1302:1305	arg1	identity					1307:1314	amino acid identity	1296:1314	amino acid identity	1296:1314	The amino acid identity and average nucleotide identity data for the whole genome proved that strain SIJ1T does indeed represent a novel genus.
33909547	8	53	theme	strain	1059:1064	arg1	SIJ1T					1066:1070	strain SIJ1T	1059:1070	strain SIJ1T	1059:1070	The DNA G+C content was 43.1 mol% and the full genome analysis for strain SIJ1T showed that it had a genome size of 3 989 945 bp and contained 4085 predicted protein-encoding genes.
33909547	12	54	theme	gen.	1748:1751	arg1	nov.					1753:1756	the name Litoribacterium kuwaitense gen. nov.	1712:1756	the name Litoribacterium kuwaitense gen. nov.	1712:1756	On the basis of the phenotypic, phylogenetic and genetic data, strain SIJ1T represents a novel genus and species in the family Bacillaceae, for which the name Litoribacterium kuwaitense gen. nov., sp.
33909547	4	55	theme	respiratory	550:560	arg1	menaquinone					485:495	an unsaturated menaquinone	470:495	an unsaturated menaquinone with seven isoprene units (MK-7)	470:528	The cell-wall peptidoglycan was based on meso-diaminopimelic acid and an unsaturated menaquinone with seven isoprene units (MK-7) was the predominant respiratory quinone.
33909547	4	55	theme	respiratory	550:560	arg1	quinone					562:568	the predominant respiratory quinone	534:568	the predominant respiratory quinone	534:568	The cell-wall peptidoglycan was based on meso-diaminopimelic acid and an unsaturated menaquinone with seven isoprene units (MK-7) was the predominant respiratory quinone.
33909547	6	56	theme	unidentified	844:855	arg1	lipid					857:861	an unidentified lipid	841:861	an unidentified lipid	841:861	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipid, an unidentified glycolipid, phosphoglycolipid and an unidentified lipid.
33909547	5	57	contain	contained	574:582	arg2	anteiso-C15 					584:595	anteiso-C15 	584:595	anteiso-C15 	584:595	It contained anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0 as the major fatty acids and ribose as the major whole-cell sugar.
33909547	5	57	contain	contained	574:582	arg1	It					571:572	It	571:572	It	571:572	It contained anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0 as the major fatty acids and ribose as the major whole-cell sugar.
33909547	5	57	contain	contained	574:582	arg2	acids					648:652	major fatty acids	636:652	major fatty acids	636:652	It contained anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0 as the major fatty acids and ribose as the major whole-cell sugar.
33909547	10	58	theme	nucleotide	1328:1337	arg1	identity					1339:1346	average nucleotide identity	1320:1346	average nucleotide identity	1320:1346	The amino acid identity and average nucleotide identity data for the whole genome proved that strain SIJ1T does indeed represent a novel genus.
33909547	2	59	theme	Kuwait	274:279	arg1	northwest					286:294	northwest	286:294	northwest of the Arabian Gulf	286:314	A Gram-stain-positive, strictly aerobic, spore-forming, rod-shaped and non-motile bacterium designated strain SIJ1T was obtained from tidal flat sediment collected from the northern shore of Kuwait Bay, northwest of the Arabian Gulf.
33909547	2	59	theme	Kuwait	274:279	arg1	Bay					281:283	Kuwait Bay	274:283	Kuwait Bay	274:283	A Gram-stain-positive, strictly aerobic, spore-forming, rod-shaped and non-motile bacterium designated strain SIJ1T was obtained from tidal flat sediment collected from the northern shore of Kuwait Bay, northwest of the Arabian Gulf.
33909547	2	60	theme	spore-forming	124:136	arg1	bacterium					165:173	A Gram-stain-positive, strictly aerobic, spore-forming, rod-shaped and non-motile bacterium	83:173	A Gram-stain-positive, strictly aerobic, spore-forming, rod-shaped and non-motile bacterium designated strain SIJ1T	83:197	A Gram-stain-positive, strictly aerobic, spore-forming, rod-shaped and non-motile bacterium designated strain SIJ1T was obtained from tidal flat sediment collected from the northern shore of Kuwait Bay, northwest of the Arabian Gulf.
33909547	6	61	theme	polar	700:704	arg1	lipids					706:711	The polar lipids	696:711	The polar lipids	696:711	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipid, an unidentified glycolipid, phosphoglycolipid and an unidentified lipid.
33909547	6	61	theme	polar	700:704	arg1	diphosphatidylglycerol					718:739	diphosphatidylglycerol	718:739	diphosphatidylglycerol	718:739	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipid, an unidentified glycolipid, phosphoglycolipid and an unidentified lipid.
33909547	4	62	with	menaquinone	485:495	arg1	MK-7					524:527	MK-7	524:527	MK-7	524:527	The cell-wall peptidoglycan was based on meso-diaminopimelic acid and an unsaturated menaquinone with seven isoprene units (MK-7) was the predominant respiratory quinone.
33909547	4	62	with	menaquinone	485:495	arg1	units					517:521	seven isoprene units	502:521	seven isoprene units (MK-7)	502:528	The cell-wall peptidoglycan was based on meso-diaminopimelic acid and an unsaturated menaquinone with seven isoprene units (MK-7) was the predominant respiratory quinone.
33909547	14	63	theme	28862T=LMG	1812:1821	arg1	SIJ1T					1800:1804	SIJ1T	1800:1804	SIJ1T (=DSM 28862T=LMG 28316T)	1800:1829	The type strain is SIJ1T (=DSM 28862T=LMG 28316T).
33909547	14	63	theme	28862T=LMG	1812:1821	arg1	28316T					1823:1828	=DSM 28862T=LMG 28316T	1807:1828	=DSM 28862T=LMG 28316T	1807:1828	The type strain is SIJ1T (=DSM 28862T=LMG 28316T).
33909547	8	64	theme	3 989 945 bp	1108:1119	arg1	size					1100:1103	a genome size	1091:1103	a genome size of 3 989 945 bp	1091:1119	The DNA G+C content was 43.1 mol% and the full genome analysis for strain SIJ1T showed that it had a genome size of 3 989 945 bp and contained 4085 predicted protein-encoding genes.
33909547	2	65	theme	flat	223:226	arg1	sediment					228:235	tidal flat sediment	217:235	tidal flat sediment collected from the northern shore of Kuwait Bay, northwest of the Arabian Gulf	217:314	A Gram-stain-positive, strictly aerobic, spore-forming, rod-shaped and non-motile bacterium designated strain SIJ1T was obtained from tidal flat sediment collected from the northern shore of Kuwait Bay, northwest of the Arabian Gulf.
33909547	9	66	theme	SIJ1T	1178:1182	arg1	genome					1194:1199	The SIJ1T annotated genome	1174:1199	The SIJ1T annotated genome	1174:1199	The SIJ1T annotated genome showed more stress resistance encoding genes in comparison to its closely related strains.
33909547	8	67	contain	contained	1125:1133	arg2	genes					1167:1171	4085 predicted protein-encoding genes	1135:1171	4085 predicted protein-encoding genes	1135:1171	The DNA G+C content was 43.1 mol% and the full genome analysis for strain SIJ1T showed that it had a genome size of 3 989 945 bp and contained 4085 predicted protein-encoding genes.
33909547	8	67	contain	contained	1125:1133	arg1	it					1084:1085	it	1084:1085	it	1084:1085	The DNA G+C content was 43.1 mol% and the full genome analysis for strain SIJ1T showed that it had a genome size of 3 989 945 bp and contained 4085 predicted protein-encoding genes.
33909547	11	68	from	genera	1501:1506	arg1	strain					1440:1445	The strain	1436:1445	The strain	1436:1445	The strain was distinguishable from the phylogenetically related genera through differences in several phenotypic properties.
33909547	11	68	from	genera	1501:1506	arg1	distinguishable					1451:1465	distinguishable	1451:1465	distinguishable	1451:1465	The strain was distinguishable from the phylogenetically related genera through differences in several phenotypic properties.
33909547	8	69	theme	predicted	1140:1148	arg1	genes					1167:1171	4085 predicted protein-encoding genes	1135:1171	4085 predicted protein-encoding genes	1135:1171	The DNA G+C content was 43.1 mol% and the full genome analysis for strain SIJ1T showed that it had a genome size of 3 989 945 bp and contained 4085 predicted protein-encoding genes.
33909547	7	70	theme	evolutionary	948:959	arg1	lineage					961:967	a distinct evolutionary lineage	937:967	a distinct evolutionary lineage	937:967	Phylogenetic analysis based on 16S rRNA genes revealed that SIJ1T showed a distinct evolutionary lineage within the Firmicutes.
33909547	11	71	from	differences	1516:1526	arg1	properties					1550:1559	several phenotypic properties	1531:1559	several phenotypic properties	1531:1559	The strain was distinguishable from the phylogenetically related genera through differences in several phenotypic properties.
33909547	12	72	theme	novel	1651:1655	arg1	genus					1657:1661	a novel genus	1649:1661	a novel genus	1649:1661	On the basis of the phenotypic, phylogenetic and genetic data, strain SIJ1T represents a novel genus and species in the family Bacillaceae, for which the name Litoribacterium kuwaitense gen. nov., sp.
33909547	9	73	theme	more	1208:1211	arg1	genes					1240:1244	more stress resistance encoding genes	1208:1244	more stress resistance encoding genes	1208:1244	The SIJ1T annotated genome showed more stress resistance encoding genes in comparison to its closely related strains.
33909547	9	74	dep	strains	1283:1289	arg1	comparison					1249:1258	comparison	1249:1258	comparison	1249:1258	The SIJ1T annotated genome showed more stress resistance encoding genes in comparison to its closely related strains.
33909547	9	75	theme	resistance	1220:1229	arg1	genes					1240:1244	more stress resistance encoding genes	1208:1244	more stress resistance encoding genes	1208:1244	The SIJ1T annotated genome showed more stress resistance encoding genes in comparison to its closely related strains.
33909547	12	76	theme	strain	1625:1630	arg1	SIJ1T					1632:1636	strain SIJ1T	1625:1636	strain SIJ1T	1625:1636	On the basis of the phenotypic, phylogenetic and genetic data, strain SIJ1T represents a novel genus and species in the family Bacillaceae, for which the name Litoribacterium kuwaitense gen. nov., sp.
33909547	5	77	theme	major	636:640	arg1	acids					648:652	major fatty acids	636:652	major fatty acids	636:652	It contained anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0 as the major fatty acids and ribose as the major whole-cell sugar.
33909547	5	77	theme	major	636:640	arg1	anteiso-C15 					584:595	anteiso-C15 	584:595	anteiso-C15 	584:595	It contained anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0 as the major fatty acids and ribose as the major whole-cell sugar.
33909547	4	78	theme	cell-wall	404:412	arg1	peptidoglycan					414:426	The cell-wall peptidoglycan	400:426	The cell-wall peptidoglycan	400:426	The cell-wall peptidoglycan was based on meso-diaminopimelic acid and an unsaturated menaquinone with seven isoprene units (MK-7) was the predominant respiratory quinone.
33909547	8	79	theme	G+C	1000:1002	arg1	content					1004:1010	The DNA G+C content	992:1010	The DNA G+C content	992:1010	The DNA G+C content was 43.1 mol% and the full genome analysis for strain SIJ1T showed that it had a genome size of 3 989 945 bp and contained 4085 predicted protein-encoding genes.
33909547	8	79	theme	G+C	1000:1002	arg1	%					1024:1024	43.1 mol%	1016:1024	43.1 mol%	1016:1024	The DNA G+C content was 43.1 mol% and the full genome analysis for strain SIJ1T showed that it had a genome size of 3 989 945 bp and contained 4085 predicted protein-encoding genes.
33909547	7	80	theme	rRNA	899:902	arg1	genes					904:908	16S rRNA genes	895:908	16S rRNA genes	895:908	Phylogenetic analysis based on 16S rRNA genes revealed that SIJ1T showed a distinct evolutionary lineage within the Firmicutes.
33909547	0	81	theme	kuwaitense	16:25	arg1	nov.					32:35	Litoribacterium kuwaitense gen. nov.	0:35	Litoribacterium kuwaitense gen. nov.	0:35	Litoribacterium kuwaitense gen. nov., sp.
33909547	1	82	theme	Kuwait	64:69	arg1	flat					77:80	a Kuwait tidal flat	62:80	a Kuwait tidal flat	62:80	nov., isolated from a Kuwait tidal flat.
33909547	3	83	theme	6 	384:385	arg1	%					386:386	%	386:386	%	386:386	Strain SIJ1T grew optimally at 30 °C and pH 7-8 in the presence of 6 % (w/v) NaCl.
33909547	9	84	theme	related	1275:1281	arg1	strains					1283:1289	its closely related strains	1263:1289	its closely related strains	1263:1289	The SIJ1T annotated genome showed more stress resistance encoding genes in comparison to its closely related strains.
33909547	12	85	dep	data	1619:1622	arg1	the					1565:1567	the	1565:1567	the	1565:1567	On the basis of the phenotypic, phylogenetic and genetic data, strain SIJ1T represents a novel genus and species in the family Bacillaceae, for which the name Litoribacterium kuwaitense gen. nov., sp.
33909547	12	85	dep	data	1619:1622	arg1	basis					1569:1573	basis	1569:1573	basis	1569:1573	On the basis of the phenotypic, phylogenetic and genetic data, strain SIJ1T represents a novel genus and species in the family Bacillaceae, for which the name Litoribacterium kuwaitense gen. nov., sp.
33909547	5	86	theme	whole-cell	678:687	arg1	sugar					689:693	the major whole-cell sugar	668:693	the major whole-cell sugar	668:693	It contained anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0 as the major fatty acids and ribose as the major whole-cell sugar.
33909547	5	87	dep	anteiso-C15 	584:595	arg1	 0					597:598	 0	597:598	 0	597:598	It contained anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0 as the major fatty acids and ribose as the major whole-cell sugar.
33909547	5	87	dep	anteiso-C15 	584:595	arg1	iso-C15 					617:624	iso-C15 	617:624	iso-C15 	617:624	It contained anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0 as the major fatty acids and ribose as the major whole-cell sugar.
33909547	5	87	dep	anteiso-C15 	584:595	arg1	 0					610:611	 0	610:611	 0	610:611	It contained anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0 as the major fatty acids and ribose as the major whole-cell sugar.
33909547	5	87	dep	anteiso-C15 	584:595	arg1	 0					626:627	 0	626:627	 0	626:627	It contained anteiso-C15 : 0, iso-C16 : 0 and iso-C15 : 0 as the major fatty acids and ribose as the major whole-cell sugar.
33909547	2	88	dep	Gram-stain-positive	85:103	arg1	aerobic					115:121	aerobic	115:121	aerobic	115:121	A Gram-stain-positive, strictly aerobic, spore-forming, rod-shaped and non-motile bacterium designated strain SIJ1T was obtained from tidal flat sediment collected from the northern shore of Kuwait Bay, northwest of the Arabian Gulf.
33909547	8	89	theme	genome	1039:1044	arg1	analysis					1046:1053	the full genome analysis	1030:1053	the full genome analysis for strain SIJ1T	1030:1070	The DNA G+C content was 43.1 mol% and the full genome analysis for strain SIJ1T showed that it had a genome size of 3 989 945 bp and contained 4085 predicted protein-encoding genes.
33909547	3	90	dep	%	386:386	arg1	w/v					389:391	w/v	389:391	w/v	389:391	Strain SIJ1T grew optimally at 30 °C and pH 7-8 in the presence of 6 % (w/v) NaCl.
33909547	11	91	theme	several	1531:1537	arg1	properties					1550:1559	several phenotypic properties	1531:1559	several phenotypic properties	1531:1559	The strain was distinguishable from the phylogenetically related genera through differences in several phenotypic properties.
33909547	2	92	theme	Gulf	311:314	arg1	northwest					286:294	northwest	286:294	northwest of the Arabian Gulf	286:314	A Gram-stain-positive, strictly aerobic, spore-forming, rod-shaped and non-motile bacterium designated strain SIJ1T was obtained from tidal flat sediment collected from the northern shore of Kuwait Bay, northwest of the Arabian Gulf.
33909547	2	92	theme	Gulf	311:314	arg1	Bay					281:283	Kuwait Bay	274:283	Kuwait Bay	274:283	A Gram-stain-positive, strictly aerobic, spore-forming, rod-shaped and non-motile bacterium designated strain SIJ1T was obtained from tidal flat sediment collected from the northern shore of Kuwait Bay, northwest of the Arabian Gulf.
33909547	4	93	theme	predominant	538:548	arg1	menaquinone					485:495	an unsaturated menaquinone	470:495	an unsaturated menaquinone with seven isoprene units (MK-7)	470:528	The cell-wall peptidoglycan was based on meso-diaminopimelic acid and an unsaturated menaquinone with seven isoprene units (MK-7) was the predominant respiratory quinone.
33909547	4	93	theme	predominant	538:548	arg1	quinone					562:568	the predominant respiratory quinone	534:568	the predominant respiratory quinone	534:568	The cell-wall peptidoglycan was based on meso-diaminopimelic acid and an unsaturated menaquinone with seven isoprene units (MK-7) was the predominant respiratory quinone.
33909547	2	94	theme	strain	186:191	arg1	SIJ1T					193:197	strain SIJ1T	186:197	strain SIJ1T	186:197	A Gram-stain-positive, strictly aerobic, spore-forming, rod-shaped and non-motile bacterium designated strain SIJ1T was obtained from tidal flat sediment collected from the northern shore of Kuwait Bay, northwest of the Arabian Gulf.
33909547	10	95	theme	identity	1307:1314	arg1	data					1348:1351	The amino acid identity and average nucleotide identity data	1292:1351	The amino acid identity and average nucleotide identity data for the whole genome	1292:1372	The amino acid identity and average nucleotide identity data for the whole genome proved that strain SIJ1T does indeed represent a novel genus.
33909547	12	96	theme	name	1716:1719	arg1	nov.					1753:1756	the name Litoribacterium kuwaitense gen. nov.	1712:1756	the name Litoribacterium kuwaitense gen. nov.	1712:1756	On the basis of the phenotypic, phylogenetic and genetic data, strain SIJ1T represents a novel genus and species in the family Bacillaceae, for which the name Litoribacterium kuwaitense gen. nov., sp.
33909547	10	97	theme	amino	1296:1300	arg1	identity					1307:1314	amino acid identity	1296:1314	amino acid identity	1296:1314	The amino acid identity and average nucleotide identity data for the whole genome proved that strain SIJ1T does indeed represent a novel genus.
33807544	8	0	theme	microbiota-derived	1135:1152	arg1	analysis					1166:1173	the targeted microbiota-derived metabolites analysis	1122:1173	the targeted microbiota-derived metabolites analysis	1122:1173	Furthermore, the targeted microbiota-derived metabolites analysis suggested that BLIDF feeding can reverse the DSS-induced decline of short-chain fatty acids and secondary bile acids in mice feces.
33807544	5	1	from	TNF-α	766:770	arg1	colon					797:801	colon	797:801	colon	797:801	Results demonstrated that feeding BLIDF markedly mitigated DSS-induced acute colitis symptoms and down-regulated IL-6, TNF-α, and IL-1β levels in the colon and serum of colitis mice.
33807544	5	1	from	TNF-α	766:770	arg1	serum					807:811	serum	807:811	serum	807:811	Results demonstrated that feeding BLIDF markedly mitigated DSS-induced acute colitis symptoms and down-regulated IL-6, TNF-α, and IL-1β levels in the colon and serum of colitis mice.
33807544	10	2	theme	metabolites	1642:1652	arg1	production					1609:1618	the production	1605:1618	the production of microbiota-derived metabolites	1605:1652	Overall, our observations suggest that BLIDF exerts anti-inflammatory effects via modulating the intestinal microbiota composition and increasing the production of microbiota-derived metabolites.
33807544	6	3	theme	Erysipelatoclostridium	948:969	arg1	abundance					919:927	the abundance	915:927	the abundance of Parasutterella, Erysipelatoclostridium, and Alistipes	915:984	BLIDF supplementation effectively reduced the abundance of Akkermansia and increased the abundance of Parasutterella, Erysipelatoclostridium, and Alistipes.
33807544	6	4	theme	Alistipes	976:984	arg1	abundance					919:927	the abundance	915:927	the abundance of Parasutterella, Erysipelatoclostridium, and Alistipes	915:984	BLIDF supplementation effectively reduced the abundance of Akkermansia and increased the abundance of Parasutterella, Erysipelatoclostridium, and Alistipes.
33807544	8	5	theme	targeted	1126:1133	arg1	analysis					1166:1173	the targeted microbiota-derived metabolites analysis	1122:1173	the targeted microbiota-derived metabolites analysis	1122:1173	Furthermore, the targeted microbiota-derived metabolites analysis suggested that BLIDF feeding can reverse the DSS-induced decline of short-chain fatty acids and secondary bile acids in mice feces.
33807544	3	6	theme	barley	467:472	arg1	BLIDF					480:484	BLIDF	480:484	BLIDF	480:484	In the present study, we investigated whether IDF from barley leaf (BLIDF) can inhibit gut inflammation via modulating the intestinal microbiota in DSS-induced colitis mice.
33807544	3	6	theme	barley	467:472	arg1	leaf					474:477	barley leaf	467:477	barley leaf (BLIDF)	467:485	In the present study, we investigated whether IDF from barley leaf (BLIDF) can inhibit gut inflammation via modulating the intestinal microbiota in DSS-induced colitis mice.
33807544	0	7	theme	Gut	105:107	arg1	Microbiota					109:118	Gut Microbiota	105:118	Gut Microbiota	105:118	Barley Leaf Insoluble Dietary Fiber Alleviated Dextran Sulfate Sodium-Induced Mice Colitis by Modulating Gut Microbiota.
33807544	5	8	from	IL-6	760:763	arg1	colon					797:801	colon	797:801	colon	797:801	Results demonstrated that feeding BLIDF markedly mitigated DSS-induced acute colitis symptoms and down-regulated IL-6, TNF-α, and IL-1β levels in the colon and serum of colitis mice.
33807544	5	8	from	IL-6	760:763	arg1	serum					807:811	serum	807:811	serum	807:811	Results demonstrated that feeding BLIDF markedly mitigated DSS-induced acute colitis symptoms and down-regulated IL-6, TNF-α, and IL-1β levels in the colon and serum of colitis mice.
33807544	10	9	link	microbiota-derived	1623:1640	arg1	metabolites					1642:1652	microbiota-derived metabolites	1623:1652	microbiota-derived metabolites	1623:1652	Overall, our observations suggest that BLIDF exerts anti-inflammatory effects via modulating the intestinal microbiota composition and increasing the production of microbiota-derived metabolites.
33807544	8	10	theme	metabolites	1154:1164	arg1	analysis					1166:1173	the targeted microbiota-derived metabolites analysis	1122:1173	the targeted microbiota-derived metabolites analysis	1122:1173	Furthermore, the targeted microbiota-derived metabolites analysis suggested that BLIDF feeding can reverse the DSS-induced decline of short-chain fatty acids and secondary bile acids in mice feces.
33807544	3	11	from	leaf	474:477	arg1	IDF					458:460	IDF	458:460	IDF from barley leaf (BLIDF)	458:485	In the present study, we investigated whether IDF from barley leaf (BLIDF) can inhibit gut inflammation via modulating the intestinal microbiota in DSS-induced colitis mice.
33807544	1	12	theme	gut	262:264	arg1	modulation					277:286	gut microbiota modulation	262:286	gut microbiota modulation	262:286	Supplementation of dietary fiber has been proved to be an effective strategy to prevent and relieve inflammatory bowel disease (IBD) through gut microbiota modulation.
33807544	5	13	from	levels	783:788	arg1	colon					797:801	colon	797:801	colon	797:801	Results demonstrated that feeding BLIDF markedly mitigated DSS-induced acute colitis symptoms and down-regulated IL-6, TNF-α, and IL-1β levels in the colon and serum of colitis mice.
33807544	5	13	from	levels	783:788	arg1	serum					807:811	serum	807:811	serum	807:811	Results demonstrated that feeding BLIDF markedly mitigated DSS-induced acute colitis symptoms and down-regulated IL-6, TNF-α, and IL-1β levels in the colon and serum of colitis mice.
33807544	5	14	theme	colitis	816:822	arg1	mice					824:827	colitis mice	816:827	colitis mice	816:827	Results demonstrated that feeding BLIDF markedly mitigated DSS-induced acute colitis symptoms and down-regulated IL-6, TNF-α, and IL-1β levels in the colon and serum of colitis mice.
33807544	4	15	theme	%	608:608	arg1	diet					629:632	1.52% BLIDF-supplemented diet	604:632	1.52% BLIDF-supplemented diet	604:632	The mice were fed 1.52% BLIDF-supplemented diet for 28 days.
33807544	5	16	theme	DSS-induced	706:716	arg1	colitis					724:730	DSS-induced acute colitis	706:730	DSS-induced acute colitis symptoms	706:739	Results demonstrated that feeding BLIDF markedly mitigated DSS-induced acute colitis symptoms and down-regulated IL-6, TNF-α, and IL-1β levels in the colon and serum of colitis mice.
33807544	6	17	theme	BLIDF	830:834	arg1	supplementation					836:850	BLIDF supplementation	830:850	BLIDF supplementation	830:850	BLIDF supplementation effectively reduced the abundance of Akkermansia and increased the abundance of Parasutterella, Erysipelatoclostridium, and Alistipes.
33807544	9	18	theme	claudin-1	1418:1426	arg1	expression					1404:1413	the expression	1400:1413	the expression of claudin-1 in colons of DSS-treated mice	1400:1456	Finally, BLIDF supplementation elevated the expression of occludin and mucin2, and decreased the expression of claudin-1 in colons of DSS-treated mice.
33807544	8	19	theme	BLIDF	1190:1194	arg1	feeding					1196:1202	BLIDF feeding	1190:1202	BLIDF feeding	1190:1202	Furthermore, the targeted microbiota-derived metabolites analysis suggested that BLIDF feeding can reverse the DSS-induced decline of short-chain fatty acids and secondary bile acids in mice feces.
33807544	8	20	theme	acids	1286:1290	arg1	decline					1232:1238	the DSS-induced decline	1216:1238	the DSS-induced decline of short-chain fatty acids and secondary bile acids in mice feces	1216:1304	Furthermore, the targeted microbiota-derived metabolites analysis suggested that BLIDF feeding can reverse the DSS-induced decline of short-chain fatty acids and secondary bile acids in mice feces.
33807544	4	21	theme	1.52	604:607	arg1	%					608:608	%	608:608	%	608:608	The mice were fed 1.52% BLIDF-supplemented diet for 28 days.
33807544	0	22	theme	Leaf	7:10	arg1	Fiber					30:34	Barley Leaf Insoluble Dietary Fiber	0:34	Barley Leaf Insoluble Dietary Fiber	0:34	Barley Leaf Insoluble Dietary Fiber Alleviated Dextran Sulfate Sodium-Induced Mice Colitis by Modulating Gut Microbiota.
33807544	10	23	theme	intestinal	1556:1565	arg1	composition					1578:1588	the intestinal microbiota composition	1552:1588	the intestinal microbiota composition	1552:1588	Overall, our observations suggest that BLIDF exerts anti-inflammatory effects via modulating the intestinal microbiota composition and increasing the production of microbiota-derived metabolites.
33807544	7	24	theme	intestinal	1058:1067	arg1	microbiota					1069:1078	the intestinal microbiota	1054:1078	the intestinal microbiota	1054:1078	Importantly, the anti-colitis effects of BLIDF were abolished when the intestinal microbiota was depleted by antibiotics.
33807544	5	25	theme	acute	718:722	arg1	colitis					724:730	DSS-induced acute colitis	706:730	DSS-induced acute colitis symptoms	706:739	Results demonstrated that feeding BLIDF markedly mitigated DSS-induced acute colitis symptoms and down-regulated IL-6, TNF-α, and IL-1β levels in the colon and serum of colitis mice.
33807544	8	26	theme	acids	1261:1265	arg1	decline					1232:1238	the DSS-induced decline	1216:1238	the DSS-induced decline of short-chain fatty acids and secondary bile acids in mice feces	1216:1304	Furthermore, the targeted microbiota-derived metabolites analysis suggested that BLIDF feeding can reverse the DSS-induced decline of short-chain fatty acids and secondary bile acids in mice feces.
33807544	8	27	from	decline	1232:1238	arg1	feces					1300:1304	mice feces	1295:1304	mice feces	1295:1304	Furthermore, the targeted microbiota-derived metabolites analysis suggested that BLIDF feeding can reverse the DSS-induced decline of short-chain fatty acids and secondary bile acids in mice feces.
33807544	1	28	theme	effective	179:187	arg1	Supplementation					121:135	Supplementation	121:135	Supplementation of dietary fiber	121:152	Supplementation of dietary fiber has been proved to be an effective strategy to prevent and relieve inflammatory bowel disease (IBD) through gut microbiota modulation.
33807544	1	28	theme	effective	179:187	arg1	strategy					189:196	an effective strategy	176:196	an effective strategy to prevent and relieve inflammatory bowel disease (IBD) through gut microbiota modulation	176:286	Supplementation of dietary fiber has been proved to be an effective strategy to prevent and relieve inflammatory bowel disease (IBD) through gut microbiota modulation.
33807544	0	29	theme	Barley	0:5	arg1	Fiber					30:34	Barley Leaf Insoluble Dietary Fiber	0:34	Barley Leaf Insoluble Dietary Fiber	0:34	Barley Leaf Insoluble Dietary Fiber Alleviated Dextran Sulfate Sodium-Induced Mice Colitis by Modulating Gut Microbiota.
33807544	1	30	theme	microbiota	266:275	arg1	modulation					277:286	gut microbiota modulation	262:286	gut microbiota modulation	262:286	Supplementation of dietary fiber has been proved to be an effective strategy to prevent and relieve inflammatory bowel disease (IBD) through gut microbiota modulation.
33807544	2	31	theme	fiber	362:366	arg1	efficacy					334:341	the efficacy	330:341	the efficacy of soluble dietary fiber	330:366	However, more attention has been paid to the efficacy of soluble dietary fiber than that of insoluble dietary fiber (IDF).
33807544	0	32	theme	Dietary	22:28	arg1	Fiber					30:34	Barley Leaf Insoluble Dietary Fiber	0:34	Barley Leaf Insoluble Dietary Fiber	0:34	Barley Leaf Insoluble Dietary Fiber Alleviated Dextran Sulfate Sodium-Induced Mice Colitis by Modulating Gut Microbiota.
33807544	6	33	theme	Parasutterella	932:945	arg1	abundance					919:927	the abundance	915:927	the abundance of Parasutterella, Erysipelatoclostridium, and Alistipes	915:984	BLIDF supplementation effectively reduced the abundance of Akkermansia and increased the abundance of Parasutterella, Erysipelatoclostridium, and Alistipes.
33807544	10	34	theme	anti-inflammatory	1511:1527	arg1	effects					1529:1535	anti-inflammatory effects	1511:1535	anti-inflammatory effects	1511:1535	Overall, our observations suggest that BLIDF exerts anti-inflammatory effects via modulating the intestinal microbiota composition and increasing the production of microbiota-derived metabolites.
33807544	9	35	theme	DSS-treated	1441:1451	arg1	mice					1453:1456	DSS-treated mice	1441:1456	DSS-treated mice	1441:1456	Finally, BLIDF supplementation elevated the expression of occludin and mucin2, and decreased the expression of claudin-1 in colons of DSS-treated mice.
33807544	3	36	theme	gut	499:501	arg1	inflammation					503:514	gut inflammation	499:514	gut inflammation	499:514	In the present study, we investigated whether IDF from barley leaf (BLIDF) can inhibit gut inflammation via modulating the intestinal microbiota in DSS-induced colitis mice.
33807544	8	37	theme	bile	1281:1284	arg1	acids					1286:1290	secondary bile acids	1271:1290	secondary bile acids	1271:1290	Furthermore, the targeted microbiota-derived metabolites analysis suggested that BLIDF feeding can reverse the DSS-induced decline of short-chain fatty acids and secondary bile acids in mice feces.
33807544	0	38	theme	Insoluble	12:20	arg1	Fiber					30:34	Barley Leaf Insoluble Dietary Fiber	0:34	Barley Leaf Insoluble Dietary Fiber	0:34	Barley Leaf Insoluble Dietary Fiber Alleviated Dextran Sulfate Sodium-Induced Mice Colitis by Modulating Gut Microbiota.
33807544	3	39	from	microbiota	546:555	arg1	mice					580:583	DSS-induced colitis mice	560:583	DSS-induced colitis mice	560:583	In the present study, we investigated whether IDF from barley leaf (BLIDF) can inhibit gut inflammation via modulating the intestinal microbiota in DSS-induced colitis mice.
33807544	8	40	theme	secondary	1271:1279	arg1	acids					1286:1290	secondary bile acids	1271:1290	secondary bile acids	1271:1290	Furthermore, the targeted microbiota-derived metabolites analysis suggested that BLIDF feeding can reverse the DSS-induced decline of short-chain fatty acids and secondary bile acids in mice feces.
33807544	8	41	theme	DSS-induced	1220:1230	arg1	decline					1232:1238	the DSS-induced decline	1216:1238	the DSS-induced decline of short-chain fatty acids and secondary bile acids in mice feces	1216:1304	Furthermore, the targeted microbiota-derived metabolites analysis suggested that BLIDF feeding can reverse the DSS-induced decline of short-chain fatty acids and secondary bile acids in mice feces.
33807544	9	42	theme	occludin	1365:1372	arg1	expression					1351:1360	the expression	1347:1360	the expression of occludin and mucin2	1347:1383	Finally, BLIDF supplementation elevated the expression of occludin and mucin2, and decreased the expression of claudin-1 in colons of DSS-treated mice.
33807544	9	43	theme	BLIDF	1316:1320	arg1	supplementation					1322:1336	BLIDF supplementation	1316:1336	BLIDF supplementation	1316:1336	Finally, BLIDF supplementation elevated the expression of occludin and mucin2, and decreased the expression of claudin-1 in colons of DSS-treated mice.
33807544	10	44	theme	microbiota-derived	1623:1640	arg1	metabolites					1642:1652	microbiota-derived metabolites	1623:1652	microbiota-derived metabolites	1623:1652	Overall, our observations suggest that BLIDF exerts anti-inflammatory effects via modulating the intestinal microbiota composition and increasing the production of microbiota-derived metabolites.
33807544	4	45	theme	BLIDF-supplemented	610:627	arg1	diet					629:632	1.52% BLIDF-supplemented diet	604:632	1.52% BLIDF-supplemented diet	604:632	The mice were fed 1.52% BLIDF-supplemented diet for 28 days.
33807544	2	46	theme	dietary	391:397	arg1	IDF					406:408	IDF	406:408	IDF	406:408	However, more attention has been paid to the efficacy of soluble dietary fiber than that of insoluble dietary fiber (IDF).
33807544	2	46	theme	dietary	391:397	arg1	fiber					399:403	insoluble dietary fiber	381:403	insoluble dietary fiber (IDF)	381:409	However, more attention has been paid to the efficacy of soluble dietary fiber than that of insoluble dietary fiber (IDF).
33807544	8	47	theme	fatty	1255:1259	arg1	acids					1261:1265	short-chain fatty acids	1243:1265	short-chain fatty acids	1243:1265	Furthermore, the targeted microbiota-derived metabolites analysis suggested that BLIDF feeding can reverse the DSS-induced decline of short-chain fatty acids and secondary bile acids in mice feces.
33807544	5	48	theme	feeding	673:679	arg1	BLIDF					681:685	feeding BLIDF	673:685	feeding BLIDF	673:685	Results demonstrated that feeding BLIDF markedly mitigated DSS-induced acute colitis symptoms and down-regulated IL-6, TNF-α, and IL-1β levels in the colon and serum of colitis mice.
33807544	5	49	theme	IL-1β	777:781	arg1	levels					783:788	IL-1β levels	777:788	IL-1β levels	777:788	Results demonstrated that feeding BLIDF markedly mitigated DSS-induced acute colitis symptoms and down-regulated IL-6, TNF-α, and IL-1β levels in the colon and serum of colitis mice.
33807544	8	50	link	microbiota-derived	1135:1152	arg1	analysis					1166:1173	the targeted microbiota-derived metabolites analysis	1122:1173	the targeted microbiota-derived metabolites analysis	1122:1173	Furthermore, the targeted microbiota-derived metabolites analysis suggested that BLIDF feeding can reverse the DSS-induced decline of short-chain fatty acids and secondary bile acids in mice feces.
33807544	2	51	theme	insoluble	381:389	arg1	IDF					406:408	IDF	406:408	IDF	406:408	However, more attention has been paid to the efficacy of soluble dietary fiber than that of insoluble dietary fiber (IDF).
33807544	2	51	theme	insoluble	381:389	arg1	fiber					399:403	insoluble dietary fiber	381:403	insoluble dietary fiber (IDF)	381:409	However, more attention has been paid to the efficacy of soluble dietary fiber than that of insoluble dietary fiber (IDF).
33807544	0	52	theme	Sulfate	55:61	arg1	Colitis					83:89	Dextran Sulfate Sodium-Induced Mice Colitis	47:89	Dextran Sulfate Sodium-Induced Mice Colitis	47:89	Barley Leaf Insoluble Dietary Fiber Alleviated Dextran Sulfate Sodium-Induced Mice Colitis by Modulating Gut Microbiota.
33807544	9	53	theme	mice	1453:1456	arg1	colons					1431:1436	colons	1431:1436	colons of DSS-treated mice	1431:1456	Finally, BLIDF supplementation elevated the expression of occludin and mucin2, and decreased the expression of claudin-1 in colons of DSS-treated mice.
33807544	9	54	from	expression	1404:1413	arg1	colons					1431:1436	colons	1431:1436	colons of DSS-treated mice	1431:1456	Finally, BLIDF supplementation elevated the expression of occludin and mucin2, and decreased the expression of claudin-1 in colons of DSS-treated mice.
33807544	5	55	theme	colitis	724:730	arg1	symptoms					732:739	DSS-induced acute colitis symptoms	706:739	DSS-induced acute colitis symptoms	706:739	Results demonstrated that feeding BLIDF markedly mitigated DSS-induced acute colitis symptoms and down-regulated IL-6, TNF-α, and IL-1β levels in the colon and serum of colitis mice.
33807544	0	56	theme	Dextran	47:53	arg1	Sulfate					55:61	Dextran Sulfate	47:61	Dextran Sulfate Sodium-Induced Mice Colitis	47:89	Barley Leaf Insoluble Dietary Fiber Alleviated Dextran Sulfate Sodium-Induced Mice Colitis by Modulating Gut Microbiota.
33807544	7	57	theme	BLIDF	1028:1032	arg1	effects					1017:1023	the anti-colitis effects	1000:1023	the anti-colitis effects of BLIDF	1000:1032	Importantly, the anti-colitis effects of BLIDF were abolished when the intestinal microbiota was depleted by antibiotics.
33807544	10	58	theme	microbiota	1567:1576	arg1	composition					1578:1588	the intestinal microbiota composition	1552:1588	the intestinal microbiota composition	1552:1588	Overall, our observations suggest that BLIDF exerts anti-inflammatory effects via modulating the intestinal microbiota composition and increasing the production of microbiota-derived metabolites.
33807544	3	59	theme	intestinal	535:544	arg1	microbiota					546:555	the intestinal microbiota	531:555	the intestinal microbiota in DSS-induced colitis mice	531:583	In the present study, we investigated whether IDF from barley leaf (BLIDF) can inhibit gut inflammation via modulating the intestinal microbiota in DSS-induced colitis mice.
33807544	8	60	theme	mice	1295:1298	arg1	feces					1300:1304	mice feces	1295:1304	mice feces	1295:1304	Furthermore, the targeted microbiota-derived metabolites analysis suggested that BLIDF feeding can reverse the DSS-induced decline of short-chain fatty acids and secondary bile acids in mice feces.
33807544	6	61	theme	Akkermansia	889:899	arg1	abundance					876:884	the abundance	872:884	the abundance of Akkermansia	872:899	BLIDF supplementation effectively reduced the abundance of Akkermansia and increased the abundance of Parasutterella, Erysipelatoclostridium, and Alistipes.
33807544	7	62	theme	anti-colitis	1004:1015	arg1	effects					1017:1023	the anti-colitis effects	1000:1023	the anti-colitis effects of BLIDF	1000:1032	Importantly, the anti-colitis effects of BLIDF were abolished when the intestinal microbiota was depleted by antibiotics.
33807544	5	63	dep	colon	797:801	arg1	the					793:795	the	793:795	the	793:795	Results demonstrated that feeding BLIDF markedly mitigated DSS-induced acute colitis symptoms and down-regulated IL-6, TNF-α, and IL-1β levels in the colon and serum of colitis mice.
33807544	1	64	theme	dietary	140:146	arg1	fiber					148:152	dietary fiber	140:152	dietary fiber	140:152	Supplementation of dietary fiber has been proved to be an effective strategy to prevent and relieve inflammatory bowel disease (IBD) through gut microbiota modulation.
33807544	5	65	theme	mice	824:827	arg1	colon					797:801	colon	797:801	colon	797:801	Results demonstrated that feeding BLIDF markedly mitigated DSS-induced acute colitis symptoms and down-regulated IL-6, TNF-α, and IL-1β levels in the colon and serum of colitis mice.
33807544	5	65	theme	mice	824:827	arg1	serum					807:811	serum	807:811	serum	807:811	Results demonstrated that feeding BLIDF markedly mitigated DSS-induced acute colitis symptoms and down-regulated IL-6, TNF-α, and IL-1β levels in the colon and serum of colitis mice.
33807544	1	66	theme	inflammatory	221:232	arg1	disease					240:246	inflammatory bowel disease	221:246	inflammatory bowel disease (IBD)	221:252	Supplementation of dietary fiber has been proved to be an effective strategy to prevent and relieve inflammatory bowel disease (IBD) through gut microbiota modulation.
33807544	1	66	theme	inflammatory	221:232	arg1	IBD					249:251	IBD	249:251	IBD	249:251	Supplementation of dietary fiber has been proved to be an effective strategy to prevent and relieve inflammatory bowel disease (IBD) through gut microbiota modulation.
33807544	1	67	theme	fiber	148:152	arg1	Supplementation					121:135	Supplementation	121:135	Supplementation of dietary fiber	121:152	Supplementation of dietary fiber has been proved to be an effective strategy to prevent and relieve inflammatory bowel disease (IBD) through gut microbiota modulation.
33807544	1	67	theme	fiber	148:152	arg1	strategy					189:196	an effective strategy	176:196	an effective strategy to prevent and relieve inflammatory bowel disease (IBD) through gut microbiota modulation	176:286	Supplementation of dietary fiber has been proved to be an effective strategy to prevent and relieve inflammatory bowel disease (IBD) through gut microbiota modulation.
33807544	1	68	theme	bowel	234:238	arg1	disease					240:246	inflammatory bowel disease	221:246	inflammatory bowel disease (IBD)	221:252	Supplementation of dietary fiber has been proved to be an effective strategy to prevent and relieve inflammatory bowel disease (IBD) through gut microbiota modulation.
33807544	1	68	theme	bowel	234:238	arg1	IBD					249:251	IBD	249:251	IBD	249:251	Supplementation of dietary fiber has been proved to be an effective strategy to prevent and relieve inflammatory bowel disease (IBD) through gut microbiota modulation.
33807544	0	69	theme	Mice	78:81	arg1	Colitis					83:89	Dextran Sulfate Sodium-Induced Mice Colitis	47:89	Dextran Sulfate Sodium-Induced Mice Colitis	47:89	Barley Leaf Insoluble Dietary Fiber Alleviated Dextran Sulfate Sodium-Induced Mice Colitis by Modulating Gut Microbiota.
33807544	3	70	theme	present	419:425	arg1	study					427:431	the present study	415:431	the present study	415:431	In the present study, we investigated whether IDF from barley leaf (BLIDF) can inhibit gut inflammation via modulating the intestinal microbiota in DSS-induced colitis mice.
33807544	3	71	theme	DSS-induced	560:570	arg1	colitis					572:578	DSS-induced colitis	560:578	DSS-induced colitis mice	560:583	In the present study, we investigated whether IDF from barley leaf (BLIDF) can inhibit gut inflammation via modulating the intestinal microbiota in DSS-induced colitis mice.
33807544	2	72	theme	dietary	354:360	arg1	fiber					362:366	soluble dietary fiber	346:366	soluble dietary fiber	346:366	However, more attention has been paid to the efficacy of soluble dietary fiber than that of insoluble dietary fiber (IDF).
33807544	8	73	theme	short-chain	1243:1253	arg1	acids					1261:1265	short-chain fatty acids	1243:1265	short-chain fatty acids	1243:1265	Furthermore, the targeted microbiota-derived metabolites analysis suggested that BLIDF feeding can reverse the DSS-induced decline of short-chain fatty acids and secondary bile acids in mice feces.
33807544	0	74	theme	Sodium-Induced	63:76	arg1	Colitis					83:89	Dextran Sulfate Sodium-Induced Mice Colitis	47:89	Dextran Sulfate Sodium-Induced Mice Colitis	47:89	Barley Leaf Insoluble Dietary Fiber Alleviated Dextran Sulfate Sodium-Induced Mice Colitis by Modulating Gut Microbiota.
33807544	3	75	theme	colitis	572:578	arg1	mice					580:583	DSS-induced colitis mice	560:583	DSS-induced colitis mice	560:583	In the present study, we investigated whether IDF from barley leaf (BLIDF) can inhibit gut inflammation via modulating the intestinal microbiota in DSS-induced colitis mice.
33807544	2	76	theme	soluble	346:352	arg1	fiber					362:366	soluble dietary fiber	346:366	soluble dietary fiber	346:366	However, more attention has been paid to the efficacy of soluble dietary fiber than that of insoluble dietary fiber (IDF).
33807544	9	77	theme	mucin2	1378:1383	arg1	expression					1351:1360	the expression	1347:1360	the expression of occludin and mucin2	1347:1383	Finally, BLIDF supplementation elevated the expression of occludin and mucin2, and decreased the expression of claudin-1 in colons of DSS-treated mice.
32416748	5	0	dep	Mbp	699:701	arg1	5.09					694:697	5.09	694:697	5.09	694:697	The genome size was about 5.09 Mbp and the DNA G+C content was 36.7 mol%.
32416748	10	1	theme	phenotypic	1221:1230	arg1	characterization					1274:1289	phenotypic, chemotaxonomic, genomic and phylogenetic characterization	1221:1289	phenotypic, chemotaxonomic, genomic and phylogenetic characterization	1221:1289	Combining the data from phenotypic, chemotaxonomic, genomic and phylogenetic characterization, it was concluded that strain SCU50T should be assigned as representing a novel species within the genus Gracilibacillus.
32416748	4	2	theme	species	580:586	arg1	status					588:593	the separate species status	567:593	the separate species status of the new isolate relative to other recognized Gracilibacillus species	567:665	Genomic average nucleotide identity and digital DNA-DNA hybridization analyses confirmed the separate species status of the new isolate relative to other recognized Gracilibacillus species.
32416748	9	3	theme	peptidoglycan	1135:1147	arg1	type					1149:1152	The cell-wall peptidoglycan type	1121:1152	The cell-wall peptidoglycan type	1121:1152	The cell-wall peptidoglycan type was A1γ based on meso-diaminopimelic acid.
32416748	4	4	theme	nucleotide	494:503	arg1	identity					505:512	average nucleotide identity	486:512	average nucleotide identity	486:512	Genomic average nucleotide identity and digital DNA-DNA hybridization analyses confirmed the separate species status of the new isolate relative to other recognized Gracilibacillus species.
32416748	7	5	contain	contained	819:827	arg2	acids					900:904	dominant fatty acids	885:904	dominant fatty acids	885:904	It contained anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0 as the dominant fatty acids and menaquinone-7 as the major respiratory quinone.
32416748	7	5	contain	contained	819:827	arg2	anteiso-C15 					829:840	anteiso-C15 	829:840	anteiso-C15 	829:840	It contained anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0 as the dominant fatty acids and menaquinone-7 as the major respiratory quinone.
32416748	7	5	contain	contained	819:827	arg1	It					816:817	It	816:817	It	816:817	It contained anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0 as the dominant fatty acids and menaquinone-7 as the major respiratory quinone.
32416748	7	6	theme	dominant	885:892	arg1	acids					900:904	dominant fatty acids	885:904	dominant fatty acids	885:904	It contained anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0 as the dominant fatty acids and menaquinone-7 as the major respiratory quinone.
32416748	7	6	theme	dominant	885:892	arg1	anteiso-C15 					829:840	anteiso-C15 	829:840	anteiso-C15 	829:840	It contained anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0 as the dominant fatty acids and menaquinone-7 as the major respiratory quinone.
32416748	8	7	theme	polar	962:966	arg1	profile					974:980	The polar lipid profile	958:980	The polar lipid profile	958:980	The polar lipid profile was examined and found to comprise diphosphatidylglycerol, phosphatidylglycerol, one unidentified phospholipid and one unidentified lipid.
32416748	4	8	theme	separate	571:578	arg1	status					588:593	the separate species status	567:593	the separate species status of the new isolate relative to other recognized Gracilibacillus species	567:665	Genomic average nucleotide identity and digital DNA-DNA hybridization analyses confirmed the separate species status of the new isolate relative to other recognized Gracilibacillus species.
32416748	5	9	theme	G+C	715:717	arg1	content					719:725	the DNA G+C content	707:725	the DNA G+C content	707:725	The genome size was about 5.09 Mbp and the DNA G+C content was 36.7 mol%.
32416748	5	9	theme	G+C	715:717	arg1	%					739:739	36.7 mol%	731:739	36.7 mol%	731:739	The genome size was about 5.09 Mbp and the DNA G+C content was 36.7 mol%.
32416748	4	10	theme	average	486:492	arg1	identity					505:512	average nucleotide identity	486:512	average nucleotide identity	486:512	Genomic average nucleotide identity and digital DNA-DNA hybridization analyses confirmed the separate species status of the new isolate relative to other recognized Gracilibacillus species.
32416748	8	11	theme	lipid	968:972	arg1	profile					974:980	The polar lipid profile	958:980	The polar lipid profile	958:980	The polar lipid profile was examined and found to comprise diphosphatidylglycerol, phosphatidylglycerol, one unidentified phospholipid and one unidentified lipid.
32416748	7	12	theme	fatty	894:898	arg1	acids					900:904	dominant fatty acids	885:904	dominant fatty acids	885:904	It contained anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0 as the dominant fatty acids and menaquinone-7 as the major respiratory quinone.
32416748	7	12	theme	fatty	894:898	arg1	anteiso-C15 					829:840	anteiso-C15 	829:840	anteiso-C15 	829:840	It contained anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0 as the dominant fatty acids and menaquinone-7 as the major respiratory quinone.
32416748	8	13	theme	unidentified	1101:1112	arg1	lipid					1114:1118	one unidentified lipid	1097:1118	one unidentified lipid	1097:1118	The polar lipid profile was examined and found to comprise diphosphatidylglycerol, phosphatidylglycerol, one unidentified phospholipid and one unidentified lipid.
32416748	5	14	theme	genome	672:677	arg1	size					679:682	The genome size	668:682	The genome size	668:682	The genome size was about 5.09 Mbp and the DNA G+C content was 36.7 mol%.
32416748	5	14	theme	genome	672:677	arg1	Mbp					699:701	Mbp	699:701	Mbp	699:701	The genome size was about 5.09 Mbp and the DNA G+C content was 36.7 mol%.
32416748	4	15	theme	digital	518:524	arg1	hybridization					534:546	digital DNA-DNA hybridization	518:546	digital DNA-DNA hybridization	518:546	Genomic average nucleotide identity and digital DNA-DNA hybridization analyses confirmed the separate species status of the new isolate relative to other recognized Gracilibacillus species.
32416748	12	16	theme	type	1549:1552	arg1	strain					1554:1559	the type strain	1545:1559	the type strain	1545:1559	nov. was first established, with SCU50T (=CGMCC 1.17336T=KCTC 43107T) as the type strain.
32416748	3	17	theme	genus	319:323	arg1	Gracilibacillus					325:339	the genus Gracilibacillus	315:339	the genus Gracilibacillus	315:339	The 16S rRNA gene sequence analysis showed that strain SCU50T belonged to the genus Gracilibacillus and was most closely related to Gracilibacillus thailandensis TP2-8T (98.1 % similarity) and Gracilibacillus orientalis XH-63T (97.7 %).
32416748	4	18	theme	Genomic	478:484	arg1	analyses					548:555	Genomic average nucleotide identity and digital DNA-DNA hybridization analyses	478:555	Genomic average nucleotide identity and digital DNA-DNA hybridization analyses	478:555	Genomic average nucleotide identity and digital DNA-DNA hybridization analyses confirmed the separate species status of the new isolate relative to other recognized Gracilibacillus species.
32416748	6	19	theme	%	777:777	arg1	pH					791:792	pH 6.5-7.5 and 25-30 °C	791:813	pH	791:792	The strain grew optimally at 10-15 % (w/v) NaCl, pH 6.5-7.5 and 25-30 °C.
32416748	6	19	theme	%	777:777	arg1	25-30 °C					806:813	25-30 °C	806:813	25-30 °C	806:813	The strain grew optimally at 10-15 % (w/v) NaCl, pH 6.5-7.5 and 25-30 °C.
32416748	6	19	theme	%	777:777	arg1	NaCl					785:788	10-15 % (w/v) NaCl	771:788	10-15 % (w/v) NaCl	771:788	The strain grew optimally at 10-15 % (w/v) NaCl, pH 6.5-7.5 and 25-30 °C.
32416748	10	20	theme	genomic	1249:1255	arg1	characterization					1274:1289	phenotypic, chemotaxonomic, genomic and phylogenetic characterization	1221:1289	phenotypic, chemotaxonomic, genomic and phylogenetic characterization	1221:1289	Combining the data from phenotypic, chemotaxonomic, genomic and phylogenetic characterization, it was concluded that strain SCU50T should be assigned as representing a novel species within the genus Gracilibacillus.
32416748	9	21	theme	cell-wall	1125:1133	arg1	type					1149:1152	The cell-wall peptidoglycan type	1121:1152	The cell-wall peptidoglycan type	1121:1152	The cell-wall peptidoglycan type was A1γ based on meso-diaminopimelic acid.
32416748	5	22	theme	36.7 mol	731:738	arg1	content					719:725	the DNA G+C content	707:725	the DNA G+C content	707:725	The genome size was about 5.09 Mbp and the DNA G+C content was 36.7 mol%.
32416748	5	22	theme	36.7 mol	731:738	arg1	%					739:739	36.7 mol%	731:739	36.7 mol%	731:739	The genome size was about 5.09 Mbp and the DNA G+C content was 36.7 mol%.
32416748	6	23	theme	10-15 	771:776	arg1	%					777:777	%	777:777	%	777:777	The strain grew optimally at 10-15 % (w/v) NaCl, pH 6.5-7.5 and 25-30 °C.
32416748	1	24	theme	saline	74:79	arg1	soil					81:84	saline soil	74:84	saline soil in Northwest China	74:103	nov., a moderate halophile isolated from saline soil in Northwest China.
32416748	10	25	theme	genus	1390:1394	arg1	Gracilibacillus					1396:1410	the genus Gracilibacillus	1386:1410	the genus Gracilibacillus	1386:1410	Combining the data from phenotypic, chemotaxonomic, genomic and phylogenetic characterization, it was concluded that strain SCU50T should be assigned as representing a novel species within the genus Gracilibacillus.
32416748	7	26	dep	anteiso-C15 	829:840	arg1	 0					875:876	 0	875:876	 0	875:876	It contained anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0 as the dominant fatty acids and menaquinone-7 as the major respiratory quinone.
32416748	7	26	dep	anteiso-C15 	829:840	arg1	 0					842:843	 0	842:843	 0	842:843	It contained anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0 as the dominant fatty acids and menaquinone-7 as the major respiratory quinone.
32416748	7	26	dep	anteiso-C15 	829:840	arg1	anteiso-C17 					862:873	anteiso-C17 	862:873	anteiso-C17 	862:873	It contained anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0 as the dominant fatty acids and menaquinone-7 as the major respiratory quinone.
32416748	7	26	dep	anteiso-C15 	829:840	arg1	 0					855:856	 0	855:856	 0	855:856	It contained anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0 as the dominant fatty acids and menaquinone-7 as the major respiratory quinone.
32416748	10	27	theme	chemotaxonomic	1233:1246	arg1	characterization					1274:1289	phenotypic, chemotaxonomic, genomic and phylogenetic characterization	1221:1289	phenotypic, chemotaxonomic, genomic and phylogenetic characterization	1221:1289	Combining the data from phenotypic, chemotaxonomic, genomic and phylogenetic characterization, it was concluded that strain SCU50T should be assigned as representing a novel species within the genus Gracilibacillus.
32416748	3	28	theme	98.1 	411:415	arg1	%					416:416	%	416:416	%	416:416	The 16S rRNA gene sequence analysis showed that strain SCU50T belonged to the genus Gracilibacillus and was most closely related to Gracilibacillus thailandensis TP2-8T (98.1 % similarity) and Gracilibacillus orientalis XH-63T (97.7 %).
32416748	1	29	attach	isolated	60:67	arg2	halophile					50:58	a moderate halophile	39:58	a moderate halophile isolated from saline soil in Northwest China	39:103	nov., a moderate halophile isolated from saline soil in Northwest China.
32416748	1	29	attach	isolated	60:67	arg1	soil					81:84	saline soil	74:84	saline soil in Northwest China	74:103	nov., a moderate halophile isolated from saline soil in Northwest China.
32416748	7	30	dep	acids	900:904	arg1	the					881:883	the	881:883	the	881:883	It contained anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0 as the dominant fatty acids and menaquinone-7 as the major respiratory quinone.
32416748	3	31	theme	%	416:416	arg1	similarity					418:427	98.1 % similarity	411:427	98.1 % similarity	411:427	The 16S rRNA gene sequence analysis showed that strain SCU50T belonged to the genus Gracilibacillus and was most closely related to Gracilibacillus thailandensis TP2-8T (98.1 % similarity) and Gracilibacillus orientalis XH-63T (97.7 %).
32416748	3	31	theme	%	416:416	arg1	TP2-8T					403:408	Gracilibacillus thailandensis TP2-8T	373:408	Gracilibacillus thailandensis TP2-8T (98.1 % similarity)	373:428	The 16S rRNA gene sequence analysis showed that strain SCU50T belonged to the genus Gracilibacillus and was most closely related to Gracilibacillus thailandensis TP2-8T (98.1 % similarity) and Gracilibacillus orientalis XH-63T (97.7 %).
32416748	4	32	theme	hybridization	534:546	arg1	analyses					548:555	Genomic average nucleotide identity and digital DNA-DNA hybridization analyses	478:555	Genomic average nucleotide identity and digital DNA-DNA hybridization analyses	478:555	Genomic average nucleotide identity and digital DNA-DNA hybridization analyses confirmed the separate species status of the new isolate relative to other recognized Gracilibacillus species.
32416748	3	33	theme	gene	254:257	arg1	analysis					268:275	The 16S rRNA gene sequence analysis	241:275	The 16S rRNA gene sequence analysis	241:275	The 16S rRNA gene sequence analysis showed that strain SCU50T belonged to the genus Gracilibacillus and was most closely related to Gracilibacillus thailandensis TP2-8T (98.1 % similarity) and Gracilibacillus orientalis XH-63T (97.7 %).
32416748	9	34	theme	meso-diaminopimelic	1171:1189	arg1	acid					1191:1194	meso-diaminopimelic acid	1171:1194	meso-diaminopimelic acid	1171:1194	The cell-wall peptidoglycan type was A1γ based on meso-diaminopimelic acid.
32416748	1	35	theme	Northwest	89:97	arg1	China					99:103	Northwest China	89:103	Northwest China	89:103	nov., a moderate halophile isolated from saline soil in Northwest China.
32416748	6	36	dep	%	777:777	arg1	w/v					780:782	w/v	780:782	w/v	780:782	The strain grew optimally at 10-15 % (w/v) NaCl, pH 6.5-7.5 and 25-30 °C.
32416748	3	37	theme	orientalis	450:459	arg1	%					474:474	97.7 %	469:474	97.7 %	469:474	The 16S rRNA gene sequence analysis showed that strain SCU50T belonged to the genus Gracilibacillus and was most closely related to Gracilibacillus thailandensis TP2-8T (98.1 % similarity) and Gracilibacillus orientalis XH-63T (97.7 %).
32416748	3	37	theme	orientalis	450:459	arg1	XH-63T					461:466	Gracilibacillus orientalis XH-63T	434:466	Gracilibacillus orientalis XH-63T (97.7 %)	434:475	The 16S rRNA gene sequence analysis showed that strain SCU50T belonged to the genus Gracilibacillus and was most closely related to Gracilibacillus thailandensis TP2-8T (98.1 % similarity) and Gracilibacillus orientalis XH-63T (97.7 %).
32416748	3	38	theme	sequence	259:266	arg1	analysis					268:275	The 16S rRNA gene sequence analysis	241:275	The 16S rRNA gene sequence analysis	241:275	The 16S rRNA gene sequence analysis showed that strain SCU50T belonged to the genus Gracilibacillus and was most closely related to Gracilibacillus thailandensis TP2-8T (98.1 % similarity) and Gracilibacillus orientalis XH-63T (97.7 %).
32416748	10	39	theme	novel	1365:1369	arg1	species					1371:1377	a novel species	1363:1377	a novel species within the genus Gracilibacillus	1363:1410	Combining the data from phenotypic, chemotaxonomic, genomic and phylogenetic characterization, it was concluded that strain SCU50T should be assigned as representing a novel species within the genus Gracilibacillus.
32416748	11	40	theme	novel	1421:1425	arg1	taxon					1427:1431	a novel taxon	1419:1431	a novel taxon	1419:1431	Thus, a novel taxon named Gracilibacillus salitolerans sp.
32416748	3	41	theme	16S	245:247	arg1	rRNA					249:252	The 16S rRNA	241:252	The 16S rRNA gene sequence analysis	241:275	The 16S rRNA gene sequence analysis showed that strain SCU50T belonged to the genus Gracilibacillus and was most closely related to Gracilibacillus thailandensis TP2-8T (98.1 % similarity) and Gracilibacillus orientalis XH-63T (97.7 %).
32416748	5	42	theme	DNA	711:713	arg1	content					719:725	the DNA G+C content	707:725	the DNA G+C content	707:725	The genome size was about 5.09 Mbp and the DNA G+C content was 36.7 mol%.
32416748	5	42	theme	DNA	711:713	arg1	%					739:739	36.7 mol%	731:739	36.7 mol%	731:739	The genome size was about 5.09 Mbp and the DNA G+C content was 36.7 mol%.
32416748	10	43	theme	phylogenetic	1261:1272	arg1	characterization					1274:1289	phenotypic, chemotaxonomic, genomic and phylogenetic characterization	1221:1289	phenotypic, chemotaxonomic, genomic and phylogenetic characterization	1221:1289	Combining the data from phenotypic, chemotaxonomic, genomic and phylogenetic characterization, it was concluded that strain SCU50T should be assigned as representing a novel species within the genus Gracilibacillus.
32416748	4	44	theme	Gracilibacillus	643:657	arg1	species					659:665	other recognized Gracilibacillus species	626:665	other recognized Gracilibacillus species	626:665	Genomic average nucleotide identity and digital DNA-DNA hybridization analyses confirmed the separate species status of the new isolate relative to other recognized Gracilibacillus species.
32416748	11	45	dep	Gracilibacillus	1439:1453	arg1	salitolerans					1455:1466	salitolerans	1455:1466	salitolerans	1455:1466	Thus, a novel taxon named Gracilibacillus salitolerans sp.
32416748	3	46	theme	rRNA	249:252	arg1	analysis					268:275	The 16S rRNA gene sequence analysis	241:275	The 16S rRNA gene sequence analysis	241:275	The 16S rRNA gene sequence analysis showed that strain SCU50T belonged to the genus Gracilibacillus and was most closely related to Gracilibacillus thailandensis TP2-8T (98.1 % similarity) and Gracilibacillus orientalis XH-63T (97.7 %).
32416748	7	47	theme	respiratory	937:947	arg1	quinone					949:955	the major respiratory quinone	927:955	the major respiratory quinone	927:955	It contained anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0 as the dominant fatty acids and menaquinone-7 as the major respiratory quinone.
32416748	3	48	theme	Gracilibacillus	434:448	arg1	%					474:474	97.7 %	469:474	97.7 %	469:474	The 16S rRNA gene sequence analysis showed that strain SCU50T belonged to the genus Gracilibacillus and was most closely related to Gracilibacillus thailandensis TP2-8T (98.1 % similarity) and Gracilibacillus orientalis XH-63T (97.7 %).
32416748	3	48	theme	Gracilibacillus	434:448	arg1	XH-63T					461:466	Gracilibacillus orientalis XH-63T	434:466	Gracilibacillus orientalis XH-63T (97.7 %)	434:475	The 16S rRNA gene sequence analysis showed that strain SCU50T belonged to the genus Gracilibacillus and was most closely related to Gracilibacillus thailandensis TP2-8T (98.1 % similarity) and Gracilibacillus orientalis XH-63T (97.7 %).
32416748	3	49	theme	Gracilibacillus	373:387	arg1	similarity					418:427	98.1 % similarity	411:427	98.1 % similarity	411:427	The 16S rRNA gene sequence analysis showed that strain SCU50T belonged to the genus Gracilibacillus and was most closely related to Gracilibacillus thailandensis TP2-8T (98.1 % similarity) and Gracilibacillus orientalis XH-63T (97.7 %).
32416748	3	49	theme	Gracilibacillus	373:387	arg1	TP2-8T					403:408	Gracilibacillus thailandensis TP2-8T	373:408	Gracilibacillus thailandensis TP2-8T (98.1 % similarity)	373:428	The 16S rRNA gene sequence analysis showed that strain SCU50T belonged to the genus Gracilibacillus and was most closely related to Gracilibacillus thailandensis TP2-8T (98.1 % similarity) and Gracilibacillus orientalis XH-63T (97.7 %).
32416748	4	50	theme	identity	505:512	arg1	analyses					548:555	Genomic average nucleotide identity and digital DNA-DNA hybridization analyses	478:555	Genomic average nucleotide identity and digital DNA-DNA hybridization analyses	478:555	Genomic average nucleotide identity and digital DNA-DNA hybridization analyses confirmed the separate species status of the new isolate relative to other recognized Gracilibacillus species.
32416748	12	51	theme	=CGMCC	1513:1518	arg1	SCU50T					1505:1510	SCU50T	1505:1510	SCU50T (=CGMCC 1.17336T=KCTC 43107T) as the type strain	1505:1559	nov. was first established, with SCU50T (=CGMCC 1.17336T=KCTC 43107T) as the type strain.
32416748	12	51	theme	=CGMCC	1513:1518	arg1	43107T					1534:1539	=CGMCC 1.17336T=KCTC 43107T	1513:1539	=CGMCC 1.17336T=KCTC 43107T	1513:1539	nov. was first established, with SCU50T (=CGMCC 1.17336T=KCTC 43107T) as the type strain.
32416748	3	52	theme	thailandensis	389:401	arg1	similarity					418:427	98.1 % similarity	411:427	98.1 % similarity	411:427	The 16S rRNA gene sequence analysis showed that strain SCU50T belonged to the genus Gracilibacillus and was most closely related to Gracilibacillus thailandensis TP2-8T (98.1 % similarity) and Gracilibacillus orientalis XH-63T (97.7 %).
32416748	3	52	theme	thailandensis	389:401	arg1	TP2-8T					403:408	Gracilibacillus thailandensis TP2-8T	373:408	Gracilibacillus thailandensis TP2-8T (98.1 % similarity)	373:428	The 16S rRNA gene sequence analysis showed that strain SCU50T belonged to the genus Gracilibacillus and was most closely related to Gracilibacillus thailandensis TP2-8T (98.1 % similarity) and Gracilibacillus orientalis XH-63T (97.7 %).
32416748	4	53	theme	DNA-DNA	526:532	arg1	hybridization					534:546	digital DNA-DNA hybridization	518:546	digital DNA-DNA hybridization	518:546	Genomic average nucleotide identity and digital DNA-DNA hybridization analyses confirmed the separate species status of the new isolate relative to other recognized Gracilibacillus species.
32416748	3	54	theme	strain	289:294	arg1	SCU50T					296:301	strain SCU50T	289:301	strain SCU50T	289:301	The 16S rRNA gene sequence analysis showed that strain SCU50T belonged to the genus Gracilibacillus and was most closely related to Gracilibacillus thailandensis TP2-8T (98.1 % similarity) and Gracilibacillus orientalis XH-63T (97.7 %).
32416748	1	55	theme	moderate	41:48	arg1	halophile					50:58	a moderate halophile	39:58	a moderate halophile isolated from saline soil in Northwest China	39:103	nov., a moderate halophile isolated from saline soil in Northwest China.
32416748	2	56	theme	soil	185:188	arg1	sample					190:195	a saline soil sample	176:195	a saline soil sample	176:195	A moderately halophilic strain, designated SCU50T, was recovered from a saline soil sample and characterized by a polyphasic approach.
32416748	10	57	from	characterization	1274:1289	arg1	data					1211:1214	the data	1207:1214	the data from phenotypic, chemotaxonomic, genomic and phylogenetic characterization	1207:1289	Combining the data from phenotypic, chemotaxonomic, genomic and phylogenetic characterization, it was concluded that strain SCU50T should be assigned as representing a novel species within the genus Gracilibacillus.
32416748	7	58	theme	major	931:935	arg1	quinone					949:955	the major respiratory quinone	927:955	the major respiratory quinone	927:955	It contained anteiso-C15 : 0, iso-C15 : 0 and anteiso-C17 : 0 as the dominant fatty acids and menaquinone-7 as the major respiratory quinone.
32416748	1	59	dep	halophile	50:58	arg1	nov.					33:36	nov.	33:36	nov.	33:36	nov., a moderate halophile isolated from saline soil in Northwest China.
32416748	4	60	theme	new	602:604	arg1	isolate					606:612	the new isolate	598:612	the new isolate relative to other recognized Gracilibacillus species	598:665	Genomic average nucleotide identity and digital DNA-DNA hybridization analyses confirmed the separate species status of the new isolate relative to other recognized Gracilibacillus species.
32416748	2	61	theme	saline	178:183	arg1	sample					190:195	a saline soil sample	176:195	a saline soil sample	176:195	A moderately halophilic strain, designated SCU50T, was recovered from a saline soil sample and characterized by a polyphasic approach.
32416748	10	62	theme	strain	1314:1319	arg1	SCU50T					1321:1326	strain SCU50T	1314:1326	strain SCU50T	1314:1326	Combining the data from phenotypic, chemotaxonomic, genomic and phylogenetic characterization, it was concluded that strain SCU50T should be assigned as representing a novel species within the genus Gracilibacillus.
32416748	1	63	from	soil	81:84	arg1	China					99:103	Northwest China	89:103	Northwest China	89:103	nov., a moderate halophile isolated from saline soil in Northwest China.
32416748	11	64	theme	Gracilibacillus	1439:1453	arg1	sp					1468:1469	Gracilibacillus salitolerans sp	1439:1469	Gracilibacillus salitolerans sp	1439:1469	Thus, a novel taxon named Gracilibacillus salitolerans sp.
32416748	4	65	theme	recognized	632:641	arg1	species					659:665	other recognized Gracilibacillus species	626:665	other recognized Gracilibacillus species	626:665	Genomic average nucleotide identity and digital DNA-DNA hybridization analyses confirmed the separate species status of the new isolate relative to other recognized Gracilibacillus species.
32416748	4	66	theme	other	626:630	arg1	species					659:665	other recognized Gracilibacillus species	626:665	other recognized Gracilibacillus species	626:665	Genomic average nucleotide identity and digital DNA-DNA hybridization analyses confirmed the separate species status of the new isolate relative to other recognized Gracilibacillus species.
32416748	4	67	theme	relative	614:621	arg1	isolate					606:612	the new isolate	598:612	the new isolate relative to other recognized Gracilibacillus species	598:665	Genomic average nucleotide identity and digital DNA-DNA hybridization analyses confirmed the separate species status of the new isolate relative to other recognized Gracilibacillus species.
32416748	8	68	theme	unidentified	1067:1078	arg1	phospholipid					1080:1091	one unidentified phospholipid	1063:1091	one unidentified phospholipid	1063:1091	The polar lipid profile was examined and found to comprise diphosphatidylglycerol, phosphatidylglycerol, one unidentified phospholipid and one unidentified lipid.
32416748	2	69	theme	halophilic	119:128	arg1	strain					130:135	A moderately halophilic strain	106:135	A moderately halophilic strain	106:135	A moderately halophilic strain, designated SCU50T, was recovered from a saline soil sample and characterized by a polyphasic approach.
32416748	2	70	theme	polyphasic	220:229	arg1	approach					231:238	a polyphasic approach	218:238	a polyphasic approach	218:238	A moderately halophilic strain, designated SCU50T, was recovered from a saline soil sample and characterized by a polyphasic approach.
32416748	12	71	theme	1.17336T=KCTC	1520:1532	arg1	SCU50T					1505:1510	SCU50T	1505:1510	SCU50T (=CGMCC 1.17336T=KCTC 43107T) as the type strain	1505:1559	nov. was first established, with SCU50T (=CGMCC 1.17336T=KCTC 43107T) as the type strain.
32416748	12	71	theme	1.17336T=KCTC	1520:1532	arg1	43107T					1534:1539	=CGMCC 1.17336T=KCTC 43107T	1513:1539	=CGMCC 1.17336T=KCTC 43107T	1513:1539	nov. was first established, with SCU50T (=CGMCC 1.17336T=KCTC 43107T) as the type strain.
32416748	4	72	theme	isolate	606:612	arg1	status					588:593	the separate species status	567:593	the separate species status of the new isolate relative to other recognized Gracilibacillus species	567:665	Genomic average nucleotide identity and digital DNA-DNA hybridization analyses confirmed the separate species status of the new isolate relative to other recognized Gracilibacillus species.
34807814	14	0	theme	%	1478:1478	arg1	values					1454:1459	digital DNA-DNA hybridization values	1424:1459	digital DNA-DNA hybridization values	1424:1459	Differentiation of strain ASG 168T from closely related species was evident from digital DNA-DNA hybridization values of 29.2 and 21.3 % with P. coralliicola and P. marina, respectively.
34807814	6	1	theme	sequence	505:512	arg1	analysis					514:521	16S rRNA gene sequence analysis	491:521	16S rRNA gene sequence analysis	491:521	16S rRNA gene sequence analysis indicated that strain ASG 168T shared 97.5 % similarity with Prauserella marina MS498T and 96.7 % with Prauserella coralliicola SCSIO 11529T.
34807814	2	2	theme	ASG	99:101	arg1	168T					103:106	strain ASG 168T	92:106	strain ASG 168T	92:106	A novel actinomycete, designated strain ASG 168T, was isolated from cave rock collected from Stegodon Sea Cave in Thailand.
34807814	2	3	from	Cave	165:168	arg1	Thailand					173:180	Thailand	173:180	Thailand	173:180	A novel actinomycete, designated strain ASG 168T, was isolated from cave rock collected from Stegodon Sea Cave in Thailand.
34807814	6	4	theme	rRNA	495:498	arg1	analysis					514:521	16S rRNA gene sequence analysis	491:521	16S rRNA gene sequence analysis	491:521	16S rRNA gene sequence analysis indicated that strain ASG 168T shared 97.5 % similarity with Prauserella marina MS498T and 96.7 % with Prauserella coralliicola SCSIO 11529T.
34807814	2	5	theme	cave	127:130	arg1	rock					132:135	cave rock	127:135	cave rock collected from Stegodon Sea Cave in Thailand	127:180	A novel actinomycete, designated strain ASG 168T, was isolated from cave rock collected from Stegodon Sea Cave in Thailand.
34807814	14	6	from	values	1454:1459	arg1	evident					1411:1417	evident	1411:1417	evident	1411:1417	Differentiation of strain ASG 168T from closely related species was evident from digital DNA-DNA hybridization values of 29.2 and 21.3 % with P. coralliicola and P. marina, respectively.
34807814	5	7	theme	genus	472:476	arg1	Prauserella					478:488	the genus Prauserella	468:488	the genus Prauserella	468:488	A polyphasic taxonomic study showed that strain ASG 168T had typical characteristics of members of the genus Prauserella.
34807814	3	8	theme	non-motile	198:207	arg1	spores					209:214	non-motile spores	198:214	non-motile spores that were oval or spherical in shape with a smooth surface	198:273	Long chains of non-motile spores that were oval or spherical in shape with a smooth surface developed on aerial mycelia.
34807814	14	9	theme	hybridization	1440:1452	arg1	values					1454:1459	digital DNA-DNA hybridization values	1424:1459	digital DNA-DNA hybridization values	1424:1459	Differentiation of strain ASG 168T from closely related species was evident from digital DNA-DNA hybridization values of 29.2 and 21.3 % with P. coralliicola and P. marina, respectively.
34807814	15	10	theme	13679T=NBRC	1656:1666	arg1	168T					1644:1647	the novel actinomycete strain ASG 168T	1610:1647	the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T)	1610:1675	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	15	10	theme	13679T=NBRC	1656:1666	arg1	114887T					1668:1674	=TBRC 13679T=NBRC 114887T	1650:1674	=TBRC 13679T=NBRC 114887T	1650:1674	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	5	11	theme	typical	430:436	arg1	characteristics					438:452	typical characteristics	430:452	typical characteristics of members of the genus Prauserella	430:488	A polyphasic taxonomic study showed that strain ASG 168T had typical characteristics of members of the genus Prauserella.
34807814	4	12	theme	irregular	338:346	arg1	elements					359:366	irregular rod-shaped elements	338:366	irregular rod-shaped elements	338:366	Substrate mycelia fragmented into irregular rod-shaped elements.
34807814	14	13	theme	digital	1424:1430	arg1	hybridization					1440:1452	digital DNA-DNA hybridization	1424:1452	digital DNA-DNA hybridization values	1424:1459	Differentiation of strain ASG 168T from closely related species was evident from digital DNA-DNA hybridization values of 29.2 and 21.3 % with P. coralliicola and P. marina, respectively.
34807814	6	14	theme	97.5 	561:565	arg1	%					566:566	%	566:566	%	566:566	16S rRNA gene sequence analysis indicated that strain ASG 168T shared 97.5 % similarity with Prauserella marina MS498T and 96.7 % with Prauserella coralliicola SCSIO 11529T.
34807814	7	15	theme	identity	684:691	arg1	values					693:698	Average nucleotide identity values	665:698	Average nucleotide identity values with P. coralliicola SCSIO 11529T and P. marina MS498T	665:753	Average nucleotide identity values with P. coralliicola SCSIO 11529T and P. marina MS498T were 82.98 and 76.08 %, respectively.
34807814	7	16	theme	Average	665:671	arg1	values					693:698	Average nucleotide identity values	665:698	Average nucleotide identity values with P. coralliicola SCSIO 11529T and P. marina MS498T	665:753	Average nucleotide identity values with P. coralliicola SCSIO 11529T and P. marina MS498T were 82.98 and 76.08 %, respectively.
34807814	6	17	theme	strain	538:543	arg1	168T					549:552	strain ASG 168T	538:552	strain ASG 168T	538:552	16S rRNA gene sequence analysis indicated that strain ASG 168T shared 97.5 % similarity with Prauserella marina MS498T and 96.7 % with Prauserella coralliicola SCSIO 11529T.
34807814	15	18	theme	Prauserella	1731:1741	arg1	sp					1755:1756	Prauserella cavernicola sp	1731:1756	Prauserella cavernicola sp	1731:1756	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	15	18	theme	Prauserella	1731:1741	arg1	strain					1704:1709	the type strain	1695:1709	the type strain of a novel species	1695:1728	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	15	19	theme	species	1722:1728	arg1	168T					1644:1647	the novel actinomycete strain ASG 168T	1610:1647	the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T)	1610:1675	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	15	19	theme	species	1722:1728	arg1	strain					1704:1709	the type strain	1695:1709	the type strain of a novel species	1695:1728	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	15	19	theme	species	1722:1728	arg1	sp					1755:1756	Prauserella cavernicola sp	1731:1756	Prauserella cavernicola sp	1731:1756	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	8	20	theme	meso-diaminopimelic	831:849	arg1	acid					851:854	meso-diaminopimelic acid	831:854	meso-diaminopimelic acid	831:854	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
34807814	5	21	theme	polyphasic	371:380	arg1	study					392:396	A polyphasic taxonomic study	369:396	A polyphasic taxonomic study	369:396	A polyphasic taxonomic study showed that strain ASG 168T had typical characteristics of members of the genus Prauserella.
34807814	14	22	theme	related	1391:1397	arg1	species					1399:1405	closely related species	1383:1405	closely related species	1383:1405	Differentiation of strain ASG 168T from closely related species was evident from digital DNA-DNA hybridization values of 29.2 and 21.3 % with P. coralliicola and P. marina, respectively.
34807814	13	23	theme	genomic	1316:1322	arg1	DNA					1324:1326	the genomic DNA	1312:1326	the genomic DNA	1312:1326	The G+C content of the genomic DNA was 70.6 mol%.
34807814	2	24	theme	Stegodon	152:159	arg1	Cave					165:168	Stegodon Sea Cave	152:168	Stegodon Sea Cave in Thailand	152:180	A novel actinomycete, designated strain ASG 168T, was isolated from cave rock collected from Stegodon Sea Cave in Thailand.
34807814	6	25	theme	SCSIO	651:655	arg1	11529T					657:662	Prauserella coralliicola SCSIO 11529T	626:662	Prauserella coralliicola SCSIO 11529T	626:662	16S rRNA gene sequence analysis indicated that strain ASG 168T shared 97.5 % similarity with Prauserella marina MS498T and 96.7 % with Prauserella coralliicola SCSIO 11529T.
34807814	14	26	theme	ASG	1369:1371	arg1	168T					1373:1376	strain ASG 168T	1362:1376	strain ASG 168T	1362:1376	Differentiation of strain ASG 168T from closely related species was evident from digital DNA-DNA hybridization values of 29.2 and 21.3 % with P. coralliicola and P. marina, respectively.
34807814	0	27	theme	Prauserella	0:10	arg1	sp					24:25	Prauserella cavernicola sp	0:25	Prauserella cavernicola sp.	0:26	Prauserella cavernicola sp.
34807814	12	28	theme	phospholipid	1070:1081	arg1	profile					1083:1089	The phospholipid profile	1066:1089	The phospholipid profile	1066:1089	The phospholipid profile consisted of phosphatidylethanolamine, phosphatidylmethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and two unknown phospholipids.
34807814	7	29	with	values	693:698	arg1	MS498T					748:753	MS498T	748:753	MS498T	748:753	Average nucleotide identity values with P. coralliicola SCSIO 11529T and P. marina MS498T were 82.98 and 76.08 %, respectively.
34807814	7	29	with	values	693:698	arg1	11529T					727:732	11529T	727:732	11529T	727:732	Average nucleotide identity values with P. coralliicola SCSIO 11529T and P. marina MS498T were 82.98 and 76.08 %, respectively.
34807814	12	30	theme	unknown	1270:1276	arg1	phospholipids					1278:1290	two unknown phospholipids	1266:1290	two unknown phospholipids	1266:1290	The phospholipid profile consisted of phosphatidylethanolamine, phosphatidylmethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and two unknown phospholipids.
34807814	5	31	contain	had	426:428	arg1	168T					421:424	strain ASG 168T	410:424	strain ASG 168T	410:424	A polyphasic taxonomic study showed that strain ASG 168T had typical characteristics of members of the genus Prauserella.
34807814	5	31	contain	had	426:428	arg2	characteristics					438:452	typical characteristics	430:452	typical characteristics of members of the genus Prauserella	430:488	A polyphasic taxonomic study showed that strain ASG 168T had typical characteristics of members of the genus Prauserella.
34807814	6	32	theme	coralliicola	638:649	arg1	11529T					657:662	Prauserella coralliicola SCSIO 11529T	626:662	Prauserella coralliicola SCSIO 11529T	626:662	16S rRNA gene sequence analysis indicated that strain ASG 168T shared 97.5 % similarity with Prauserella marina MS498T and 96.7 % with Prauserella coralliicola SCSIO 11529T.
34807814	15	33	theme	=TBRC	1650:1654	arg1	168T					1644:1647	the novel actinomycete strain ASG 168T	1610:1647	the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T)	1610:1675	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	15	33	theme	=TBRC	1650:1654	arg1	114887T					1668:1674	=TBRC 13679T=NBRC 114887T	1650:1674	=TBRC 13679T=NBRC 114887T	1650:1674	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	11	34	theme	predominant	968:978	arg1	iso-C16 					997:1004	iso-C16 	997:1004	iso-C16 	997:1004	The predominant fatty acids were iso-C16 : 0, C16 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
34807814	11	34	theme	predominant	968:978	arg1	acids					986:990	The predominant fatty acids	964:990	The predominant fatty acids	964:990	The predominant fatty acids were iso-C16 : 0, C16 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
34807814	11	35	theme	 1	1057:1058	arg1	ω6c					1060:1062	 1 ω6c	1057:1062	C16 : 1 ω7c/C16 : 1 ω6c	1040:1062	The predominant fatty acids were iso-C16 : 0, C16 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
34807814	12	36	theme	phosphatidylinositol	1230:1249	arg1	mannosides					1251:1260	phosphatidylinositol mannosides	1230:1260	phosphatidylinositol mannosides	1230:1260	The phospholipid profile consisted of phosphatidylethanolamine, phosphatidylmethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and two unknown phospholipids.
34807814	5	37	theme	ASG	417:419	arg1	168T					421:424	strain ASG 168T	410:424	strain ASG 168T	410:424	A polyphasic taxonomic study showed that strain ASG 168T had typical characteristics of members of the genus Prauserella.
34807814	3	38	from	shape	247:251	arg1	oval					226:229	oval	226:229	oval	226:229	Long chains of non-motile spores that were oval or spherical in shape with a smooth surface developed on aerial mycelia.
34807814	2	39	attach	isolated	113:120	arg2	actinomycete					67:78	A novel actinomycete	59:78	A novel actinomycete	59:78	A novel actinomycete, designated strain ASG 168T, was isolated from cave rock collected from Stegodon Sea Cave in Thailand.
34807814	2	39	attach	isolated	113:120	arg1	rock					132:135	cave rock	127:135	cave rock collected from Stegodon Sea Cave in Thailand	127:180	A novel actinomycete, designated strain ASG 168T, was isolated from cave rock collected from Stegodon Sea Cave in Thailand.
34807814	2	40	theme	Sea	161:163	arg1	Cave					165:168	Stegodon Sea Cave	152:168	Stegodon Sea Cave in Thailand	152:180	A novel actinomycete, designated strain ASG 168T, was isolated from cave rock collected from Stegodon Sea Cave in Thailand.
34807814	15	41	theme	strain	1633:1638	arg1	168T					1644:1647	the novel actinomycete strain ASG 168T	1610:1647	the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T)	1610:1675	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	15	41	theme	strain	1633:1638	arg1	114887T					1668:1674	=TBRC 13679T=NBRC 114887T	1650:1674	=TBRC 13679T=NBRC 114887T	1650:1674	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	15	41	theme	strain	1633:1638	arg1	strain					1704:1709	the type strain	1695:1709	the type strain of a novel species	1695:1728	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	15	42	theme	comparative	1539:1549	arg1	analysis					1551:1558	comparative analysis	1539:1558	comparative analysis of phenotypic, chemotaxonomic and genotypic data	1539:1607	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	14	43	from	species	1399:1405	arg1	Differentiation					1343:1357	Differentiation	1343:1357	Differentiation of strain ASG 168T from closely related species	1343:1405	Differentiation of strain ASG 168T from closely related species was evident from digital DNA-DNA hybridization values of 29.2 and 21.3 % with P. coralliicola and P. marina, respectively.
34807814	15	44	theme	novel	1614:1618	arg1	168T					1644:1647	the novel actinomycete strain ASG 168T	1610:1647	the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T)	1610:1675	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	15	44	theme	novel	1614:1618	arg1	114887T					1668:1674	=TBRC 13679T=NBRC 114887T	1650:1674	=TBRC 13679T=NBRC 114887T	1650:1674	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	15	44	theme	novel	1614:1618	arg1	strain					1704:1709	the type strain	1695:1709	the type strain of a novel species	1695:1728	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	3	45	from	oval	226:229	arg1	shape					247:251	shape	247:251	shape	247:251	Long chains of non-motile spores that were oval or spherical in shape with a smooth surface developed on aerial mycelia.
34807814	6	46	theme	gene	500:503	arg1	analysis					514:521	16S rRNA gene sequence analysis	491:521	16S rRNA gene sequence analysis	491:521	16S rRNA gene sequence analysis indicated that strain ASG 168T shared 97.5 % similarity with Prauserella marina MS498T and 96.7 % with Prauserella coralliicola SCSIO 11529T.
34807814	2	47	theme	strain	92:97	arg1	168T					103:106	strain ASG 168T	92:106	strain ASG 168T	92:106	A novel actinomycete, designated strain ASG 168T, was isolated from cave rock collected from Stegodon Sea Cave in Thailand.
34807814	11	48	dep	iso-C16 	997:1004	arg1	 0					1015:1016	 0	1015:1016	 0	1015:1016	The predominant fatty acids were iso-C16 : 0, C16 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
34807814	11	48	dep	iso-C16 	997:1004	arg1	feature					1029:1035	summed feature	1022:1035	summed feature	1022:1035	The predominant fatty acids were iso-C16 : 0, C16 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
34807814	11	48	dep	iso-C16 	997:1004	arg1	C16 					1010:1013	C16 	1010:1013	C16 	1010:1013	The predominant fatty acids were iso-C16 : 0, C16 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
34807814	3	49	theme	Long	183:186	arg1	chains					188:193	Long chains	183:193	Long chains of non-motile spores that were oval or spherical in shape with a smooth surface	183:273	Long chains of non-motile spores that were oval or spherical in shape with a smooth surface developed on aerial mycelia.
34807814	6	50	theme	16S	491:493	arg1	rRNA					495:498	16S rRNA	491:498	16S rRNA gene sequence analysis	491:521	16S rRNA gene sequence analysis indicated that strain ASG 168T shared 97.5 % similarity with Prauserella marina MS498T and 96.7 % with Prauserella coralliicola SCSIO 11529T.
34807814	15	51	theme	chemotaxonomic	1575:1588	arg1	data					1604:1607	phenotypic, chemotaxonomic and genotypic data	1563:1607	phenotypic, chemotaxonomic and genotypic data	1563:1607	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	15	52	theme	genotypic	1594:1602	arg1	data					1604:1607	phenotypic, chemotaxonomic and genotypic data	1563:1607	phenotypic, chemotaxonomic and genotypic data	1563:1607	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	15	53	theme	phenotypic	1563:1572	arg1	data					1604:1607	phenotypic, chemotaxonomic and genotypic data	1563:1607	phenotypic, chemotaxonomic and genotypic data	1563:1607	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	5	54	theme	Prauserella	478:488	arg1	members					457:463	members	457:463	members of the genus Prauserella	457:488	A polyphasic taxonomic study showed that strain ASG 168T had typical characteristics of members of the genus Prauserella.
34807814	11	55	theme	summed	1022:1027	arg1	feature					1029:1035	summed feature	1022:1035	summed feature	1022:1035	The predominant fatty acids were iso-C16 : 0, C16 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
34807814	3	56	theme	spores	209:214	arg1	chains					188:193	Long chains	183:193	Long chains of non-motile spores that were oval or spherical in shape with a smooth surface	183:273	Long chains of non-motile spores that were oval or spherical in shape with a smooth surface developed on aerial mycelia.
34807814	4	57	theme	rod-shaped	348:357	arg1	elements					359:366	irregular rod-shaped elements	338:366	irregular rod-shaped elements	338:366	Substrate mycelia fragmented into irregular rod-shaped elements.
34807814	14	58	theme	DNA-DNA	1432:1438	arg1	hybridization					1440:1452	digital DNA-DNA hybridization	1424:1452	digital DNA-DNA hybridization values	1424:1459	Differentiation of strain ASG 168T from closely related species was evident from digital DNA-DNA hybridization values of 29.2 and 21.3 % with P. coralliicola and P. marina, respectively.
34807814	6	59	theme	%	566:566	arg1	similarity					568:577	97.5 % similarity	561:577	97.5 % similarity	561:577	16S rRNA gene sequence analysis indicated that strain ASG 168T shared 97.5 % similarity with Prauserella marina MS498T and 96.7 % with Prauserella coralliicola SCSIO 11529T.
34807814	11	60	dep	 0	1015:1016	arg1	3					1037:1037	3	1037:1037	3	1037:1037	The predominant fatty acids were iso-C16 : 0, C16 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
34807814	11	60	dep	 0	1015:1016	arg1	C16 					1040:1043	C16 	1040:1043	C16 : 1 ω7c/C16 : 1 ω6c	1040:1062	The predominant fatty acids were iso-C16 : 0, C16 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
34807814	11	61	theme	 1	1045:1046	arg1	ω7c/C16 					1048:1055	 1 ω7c/C16 	1045:1055	C16 : 1 ω7c/C16 : 1 ω6c	1040:1062	The predominant fatty acids were iso-C16 : 0, C16 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
34807814	5	62	theme	members	457:463	arg1	characteristics					438:452	typical characteristics	430:452	typical characteristics of members of the genus Prauserella	430:488	A polyphasic taxonomic study showed that strain ASG 168T had typical characteristics of members of the genus Prauserella.
34807814	6	63	theme	ASG	545:547	arg1	168T					549:552	strain ASG 168T	538:552	strain ASG 168T	538:552	16S rRNA gene sequence analysis indicated that strain ASG 168T shared 97.5 % similarity with Prauserella marina MS498T and 96.7 % with Prauserella coralliicola SCSIO 11529T.
34807814	7	64	theme	nucleotide	673:682	arg1	values					693:698	Average nucleotide identity values	665:698	Average nucleotide identity values with P. coralliicola SCSIO 11529T and P. marina MS498T	665:753	Average nucleotide identity values with P. coralliicola SCSIO 11529T and P. marina MS498T were 82.98 and 76.08 %, respectively.
34807814	9	65	contain	contained	879:887	arg1	sugars					872:877	The whole-cell sugars	857:877	The whole-cell sugars	857:877	The whole-cell sugars contained ribose, arabinose and galactose.
34807814	9	65	contain	contained	879:887	arg2	galactose					911:919	galactose	911:919	galactose	911:919	The whole-cell sugars contained ribose, arabinose and galactose.
34807814	9	65	contain	contained	879:887	arg2	arabinose					897:905	arabinose	897:905	arabinose	897:905	The whole-cell sugars contained ribose, arabinose and galactose.
34807814	9	65	contain	contained	879:887	arg2	ribose					889:894	ribose	889:894	ribose	889:894	The whole-cell sugars contained ribose, arabinose and galactose.
34807814	3	66	theme	smooth	260:265	arg1	surface					267:273	a smooth surface	258:273	a smooth surface	258:273	Long chains of non-motile spores that were oval or spherical in shape with a smooth surface developed on aerial mycelia.
34807814	6	67	theme	Prauserella	626:636	arg1	11529T					657:662	Prauserella coralliicola SCSIO 11529T	626:662	Prauserella coralliicola SCSIO 11529T	626:662	16S rRNA gene sequence analysis indicated that strain ASG 168T shared 97.5 % similarity with Prauserella marina MS498T and 96.7 % with Prauserella coralliicola SCSIO 11529T.
34807814	2	68	theme	novel	61:65	arg1	actinomycete					67:78	A novel actinomycete	59:78	A novel actinomycete	59:78	A novel actinomycete, designated strain ASG 168T, was isolated from cave rock collected from Stegodon Sea Cave in Thailand.
34807814	11	69	dep	C16 	1040:1043	arg1	ω6c					1060:1062	 1 ω6c	1057:1062	C16 : 1 ω7c/C16 : 1 ω6c	1040:1062	The predominant fatty acids were iso-C16 : 0, C16 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
34807814	11	69	dep	C16 	1040:1043	arg1	ω7c/C16 					1048:1055	 1 ω7c/C16 	1045:1055	C16 : 1 ω7c/C16 : 1 ω6c	1040:1062	The predominant fatty acids were iso-C16 : 0, C16 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
34807814	10	70	theme	predominant	926:936	arg1	MK-9					954:957	MK-9	954:957	MK-9(H4)	954:961	The predominant menaquinone was MK-9(H4).
34807814	10	70	theme	predominant	926:936	arg1	menaquinone					938:948	The predominant menaquinone	922:948	The predominant menaquinone	922:948	The predominant menaquinone was MK-9(H4).
34807814	15	71	theme	novel	1716:1720	arg1	species					1722:1728	a novel species	1714:1728	a novel species	1714:1728	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	8	72	theme	cell-wall	797:805	arg1	peptidoglycan					807:819	The cell-wall peptidoglycan	793:819	The cell-wall peptidoglycan	793:819	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
34807814	3	73	theme	aerial	288:293	arg1	mycelia					295:301	aerial mycelia	288:301	aerial mycelia	288:301	Long chains of non-motile spores that were oval or spherical in shape with a smooth surface developed on aerial mycelia.
34807814	15	74	theme	type	1699:1702	arg1	168T					1644:1647	the novel actinomycete strain ASG 168T	1610:1647	the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T)	1610:1675	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	15	74	theme	type	1699:1702	arg1	strain					1704:1709	the type strain	1695:1709	the type strain of a novel species	1695:1728	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	15	74	theme	type	1699:1702	arg1	sp					1755:1756	Prauserella cavernicola sp	1731:1756	Prauserella cavernicola sp	1731:1756	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	13	75	theme	DNA	1324:1326	arg1	content					1301:1307	The G+C content	1293:1307	The G+C content of the genomic DNA	1293:1326	The G+C content of the genomic DNA was 70.6 mol%.
34807814	13	75	theme	DNA	1324:1326	arg1	%					1340:1340	70.6 mol%	1332:1340	70.6 mol%	1332:1340	The G+C content of the genomic DNA was 70.6 mol%.
34807814	1	76	theme	cave	48:51	arg1	rock					53:56	cave rock	48:56	cave rock	48:56	nov., isolated from cave rock.
34807814	14	77	theme	168T	1373:1376	arg1	Differentiation					1343:1357	Differentiation	1343:1357	Differentiation of strain ASG 168T from closely related species	1343:1405	Differentiation of strain ASG 168T from closely related species was evident from digital DNA-DNA hybridization values of 29.2 and 21.3 % with P. coralliicola and P. marina, respectively.
34807814	13	78	theme	70.6 mol	1332:1339	arg1	content					1301:1307	The G+C content	1293:1307	The G+C content of the genomic DNA	1293:1326	The G+C content of the genomic DNA was 70.6 mol%.
34807814	13	78	theme	70.6 mol	1332:1339	arg1	%					1340:1340	70.6 mol%	1332:1340	70.6 mol%	1332:1340	The G+C content of the genomic DNA was 70.6 mol%.
34807814	14	79	theme	strain	1362:1367	arg1	168T					1373:1376	strain ASG 168T	1362:1376	strain ASG 168T	1362:1376	Differentiation of strain ASG 168T from closely related species was evident from digital DNA-DNA hybridization values of 29.2 and 21.3 % with P. coralliicola and P. marina, respectively.
34807814	0	80	theme	cavernicola	12:22	arg1	sp					24:25	Prauserella cavernicola sp	0:25	Prauserella cavernicola sp.	0:26	Prauserella cavernicola sp.
34807814	3	81	with	oval	226:229	arg1	surface					267:273	a smooth surface	258:273	a smooth surface	258:273	Long chains of non-motile spores that were oval or spherical in shape with a smooth surface developed on aerial mycelia.
34807814	9	82	theme	whole-cell	861:870	arg1	sugars					872:877	The whole-cell sugars	857:877	The whole-cell sugars	857:877	The whole-cell sugars contained ribose, arabinose and galactose.
34807814	13	83	theme	G+C	1297:1299	arg1	content					1301:1307	The G+C content	1293:1307	The G+C content of the genomic DNA	1293:1326	The G+C content of the genomic DNA was 70.6 mol%.
34807814	13	83	theme	G+C	1297:1299	arg1	%					1340:1340	70.6 mol%	1332:1340	70.6 mol%	1332:1340	The G+C content of the genomic DNA was 70.6 mol%.
34807814	15	84	theme	cavernicola	1743:1753	arg1	sp					1755:1756	Prauserella cavernicola sp	1731:1756	Prauserella cavernicola sp	1731:1756	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	15	84	theme	cavernicola	1743:1753	arg1	strain					1704:1709	the type strain	1695:1709	the type strain of a novel species	1695:1728	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	8	85	contain	contained	821:829	arg1	peptidoglycan					807:819	The cell-wall peptidoglycan	793:819	The cell-wall peptidoglycan	793:819	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
34807814	8	85	contain	contained	821:829	arg2	acid					851:854	meso-diaminopimelic acid	831:854	meso-diaminopimelic acid	831:854	The cell-wall peptidoglycan contained meso-diaminopimelic acid.
34807814	14	86	with	%	1478:1478	arg1	coralliicola					1488:1499	P. coralliicola	1485:1499	P. coralliicola	1485:1499	Differentiation of strain ASG 168T from closely related species was evident from digital DNA-DNA hybridization values of 29.2 and 21.3 % with P. coralliicola and P. marina, respectively.
34807814	14	86	with	%	1478:1478	arg1	marina					1508:1513	P. marina	1505:1513	P. marina	1505:1513	Differentiation of strain ASG 168T from closely related species was evident from digital DNA-DNA hybridization values of 29.2 and 21.3 % with P. coralliicola and P. marina, respectively.
34807814	15	87	theme	ASG	1640:1642	arg1	168T					1644:1647	the novel actinomycete strain ASG 168T	1610:1647	the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T)	1610:1675	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	15	87	theme	ASG	1640:1642	arg1	114887T					1668:1674	=TBRC 13679T=NBRC 114887T	1650:1674	=TBRC 13679T=NBRC 114887T	1650:1674	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	15	87	theme	ASG	1640:1642	arg1	strain					1704:1709	the type strain	1695:1709	the type strain of a novel species	1695:1728	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	5	88	theme	strain	410:415	arg1	168T					421:424	strain ASG 168T	410:424	strain ASG 168T	410:424	A polyphasic taxonomic study showed that strain ASG 168T had typical characteristics of members of the genus Prauserella.
34807814	11	89	theme	fatty	980:984	arg1	iso-C16 					997:1004	iso-C16 	997:1004	iso-C16 	997:1004	The predominant fatty acids were iso-C16 : 0, C16 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
34807814	11	89	theme	fatty	980:984	arg1	acids					986:990	The predominant fatty acids	964:990	The predominant fatty acids	964:990	The predominant fatty acids were iso-C16 : 0, C16 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
34807814	4	90	theme	Substrate	304:312	arg1	mycelia					314:320	Substrate mycelia	304:320	Substrate mycelia	304:320	Substrate mycelia fragmented into irregular rod-shaped elements.
34807814	15	91	theme	actinomycete	1620:1631	arg1	168T					1644:1647	the novel actinomycete strain ASG 168T	1610:1647	the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T)	1610:1675	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	15	91	theme	actinomycete	1620:1631	arg1	114887T					1668:1674	=TBRC 13679T=NBRC 114887T	1650:1674	=TBRC 13679T=NBRC 114887T	1650:1674	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	15	91	theme	actinomycete	1620:1631	arg1	strain					1704:1709	the type strain	1695:1709	the type strain of a novel species	1695:1728	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	15	92	theme	data	1604:1607	arg1	analysis					1551:1558	comparative analysis	1539:1558	comparative analysis of phenotypic, chemotaxonomic and genotypic data	1539:1607	Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168T (=TBRC 13679T=NBRC 114887T) is proposed to be the type strain of a novel species, Prauserella cavernicola sp.
34807814	5	93	theme	taxonomic	382:390	arg1	study					392:396	A polyphasic taxonomic study	369:396	A polyphasic taxonomic study	369:396	A polyphasic taxonomic study showed that strain ASG 168T had typical characteristics of members of the genus Prauserella.
34641571	7	0	dep	showed	1038:1043	arg1	decreased					1059:1067	decreased	1059:1067	showed significantly decreased NO production and gene expression in a dose-dependent manner in LPS-stimulated RAW264.7 cells	1038:1161	Conversely, the anti-inflammatory activity of the A. personatus lipid was also analyzed and the results showed significantly decreased NO production and gene expression in a dose-dependent manner in LPS-stimulated RAW264.7 cells.
34641571	7	1	from	manner	1123:1128	arg1	cells					1157:1161	LPS-stimulated RAW264.7 cells	1133:1161	LPS-stimulated RAW264.7 cells	1133:1161	Conversely, the anti-inflammatory activity of the A. personatus lipid was also analyzed and the results showed significantly decreased NO production and gene expression in a dose-dependent manner in LPS-stimulated RAW264.7 cells.
34641571	10	2	theme	A.	1474:1475	arg1	source					1513:1518	a potential source	1501:1518	a potential source of immunomodulation	1501:1538	The results indicate that A. personatus egg lipid is a potential source of immunomodulation.
34641571	10	2	theme	A.	1474:1475	arg1	lipid					1492:1496	A. personatus egg lipid	1474:1496	A. personatus egg lipid	1474:1496	The results indicate that A. personatus egg lipid is a potential source of immunomodulation.
34641571	7	3	theme	anti-inflammatory	950:966	arg1	activity					968:975	the anti-inflammatory activity	946:975	the anti-inflammatory activity of the A. personatus lipid	946:1002	Conversely, the anti-inflammatory activity of the A. personatus lipid was also analyzed and the results showed significantly decreased NO production and gene expression in a dose-dependent manner in LPS-stimulated RAW264.7 cells.
34641571	3	4	theme	acids	451:455	arg1	content					418:424	high content	413:424	high content of polyunsaturated fatty acids (PUFAs)	413:463	The results showed that the extracted lipid contained high content of polyunsaturated fatty acids (PUFAs).
34641571	8	5	dep	A.	1181:1182	arg1	personatus					1184:1193	personatus	1184:1193	personatus	1184:1193	In addition, the A. personatus lipid suppressed the LPS-induced phosphorylation of proteins related to the NF-κB and MAPK pathways in LPS-stimulated RAW264.7 cells.
34641571	9	6	theme	CD86	1431:1434	arg1	expression					1436:1445	CD86 expression	1431:1445	CD86 expression	1431:1445	Further, flow cytometry demonstrated the lipid-regulated anti-inflammatory activity via inhibition of CD86 expression.
34641571	8	7	theme	A.	1181:1182	arg1	lipid					1195:1199	the A. personatus lipid	1177:1199	the A. personatus lipid	1177:1199	In addition, the A. personatus lipid suppressed the LPS-induced phosphorylation of proteins related to the NF-κB and MAPK pathways in LPS-stimulated RAW264.7 cells.
34641571	0	8	from	Activities	17:26	arg1	Cells					74:78	RAW264.7 Cells	65:78	RAW264.7 Cells	65:78	Immunomodulatory Activities of Ammodytes personatus Egg Lipid in RAW264.7 Cells.
34641571	1	9	theme	cold	147:150	arg1	areas					152:156	cold areas	147:156	cold areas of the North Pacific	147:177	Ammodytes personatus, known as the Pacific sand lance, thrives in cold areas of the North Pacific.
34641571	4	10	theme	lipid	519:523	arg1	activities					487:496	The immunomodulatory activities	466:496	The immunomodulatory activities of the A. personatus lipid	466:523	The immunomodulatory activities of the A. personatus lipid were investigated using rodent macrophages.
34641571	5	11	theme	NO	686:687	arg1	production					689:698	the NO production	682:698	the NO production in RAW264.7 cells	682:716	First, immune enhancement was analyzed, and the A. personatus lipid significantly and dose-dependently increased the NO production in RAW264.7 cells, and this lipid also regulated the transcription of immune-associated genes in RAW264.7 cells by activating the NF-κB and MAPK pathways.
34641571	7	12	theme	gene	1087:1090	arg1	expression					1092:1101	gene expression	1087:1101	gene expression	1087:1101	Conversely, the anti-inflammatory activity of the A. personatus lipid was also analyzed and the results showed significantly decreased NO production and gene expression in a dose-dependent manner in LPS-stimulated RAW264.7 cells.
34641571	8	13	from	pathways	1286:1293	arg1	cells					1322:1326	LPS-stimulated RAW264.7 cells	1298:1326	LPS-stimulated RAW264.7 cells	1298:1326	In addition, the A. personatus lipid suppressed the LPS-induced phosphorylation of proteins related to the NF-κB and MAPK pathways in LPS-stimulated RAW264.7 cells.
34641571	2	14	dep	chromatography	305:318	arg1	detection					342:350	-flame ionization detection	324:350	gas chromatography (GC)-flame ionization detection (FID)	301:356	In this study, the total lipid was extracted from A. personatus eggs and the fatty acid composition was determined using gas chromatography (GC)-flame ionization detection (FID).
34641571	2	14	dep	chromatography	305:318	arg1	FID					353:355	FID	353:355	FID	353:355	In this study, the total lipid was extracted from A. personatus eggs and the fatty acid composition was determined using gas chromatography (GC)-flame ionization detection (FID).
34641571	5	15	dep	A.	617:618	arg1	personatus					620:629	personatus	620:629	personatus	620:629	First, immune enhancement was analyzed, and the A. personatus lipid significantly and dose-dependently increased the NO production in RAW264.7 cells, and this lipid also regulated the transcription of immune-associated genes in RAW264.7 cells by activating the NF-κB and MAPK pathways.
34641571	3	16	theme	polyunsaturated	429:443	arg1	PUFAs					458:462	PUFAs	458:462	PUFAs	458:462	The results showed that the extracted lipid contained high content of polyunsaturated fatty acids (PUFAs).
34641571	3	16	theme	polyunsaturated	429:443	arg1	acids					451:455	polyunsaturated fatty acids	429:455	polyunsaturated fatty acids (PUFAs)	429:463	The results showed that the extracted lipid contained high content of polyunsaturated fatty acids (PUFAs).
34641571	8	17	theme	proteins	1247:1254	arg1	phosphorylation					1228:1242	the LPS-induced phosphorylation	1212:1242	the LPS-induced phosphorylation of proteins related to the NF-κB and MAPK pathways in LPS-stimulated RAW264.7 cells	1212:1326	In addition, the A. personatus lipid suppressed the LPS-induced phosphorylation of proteins related to the NF-κB and MAPK pathways in LPS-stimulated RAW264.7 cells.
34641571	5	18	theme	RAW264.7	797:804	arg1	cells					806:810	RAW264.7 cells	797:810	RAW264.7 cells	797:810	First, immune enhancement was analyzed, and the A. personatus lipid significantly and dose-dependently increased the NO production in RAW264.7 cells, and this lipid also regulated the transcription of immune-associated genes in RAW264.7 cells by activating the NF-κB and MAPK pathways.
34641571	5	19	theme	NF-κB	830:834	arg1	pathways					845:852	the NF-κB and MAPK pathways	826:852	pathways	845:852	First, immune enhancement was analyzed, and the A. personatus lipid significantly and dose-dependently increased the NO production in RAW264.7 cells, and this lipid also regulated the transcription of immune-associated genes in RAW264.7 cells by activating the NF-κB and MAPK pathways.
34641571	5	20	theme	RAW264.7	703:710	arg1	cells					712:716	RAW264.7 cells	703:716	RAW264.7 cells	703:716	First, immune enhancement was analyzed, and the A. personatus lipid significantly and dose-dependently increased the NO production in RAW264.7 cells, and this lipid also regulated the transcription of immune-associated genes in RAW264.7 cells by activating the NF-κB and MAPK pathways.
34641571	5	21	from	transcription	753:765	arg1	cells					806:810	RAW264.7 cells	797:810	RAW264.7 cells	797:810	First, immune enhancement was analyzed, and the A. personatus lipid significantly and dose-dependently increased the NO production in RAW264.7 cells, and this lipid also regulated the transcription of immune-associated genes in RAW264.7 cells by activating the NF-κB and MAPK pathways.
34641571	5	22	theme	immune	576:581	arg1	enhancement					583:593	immune enhancement	576:593	immune enhancement	576:593	First, immune enhancement was analyzed, and the A. personatus lipid significantly and dose-dependently increased the NO production in RAW264.7 cells, and this lipid also regulated the transcription of immune-associated genes in RAW264.7 cells by activating the NF-κB and MAPK pathways.
34641571	8	23	theme	MAPK	1281:1284	arg1	pathways					1286:1293	the NF-κB and MAPK pathways	1267:1293	pathways	1286:1293	In addition, the A. personatus lipid suppressed the LPS-induced phosphorylation of proteins related to the NF-κB and MAPK pathways in LPS-stimulated RAW264.7 cells.
34641571	2	24	theme	A.	230:231	arg1	eggs					244:247	A. personatus eggs	230:247	A. personatus eggs	230:247	In this study, the total lipid was extracted from A. personatus eggs and the fatty acid composition was determined using gas chromatography (GC)-flame ionization detection (FID).
34641571	8	25	theme	LPS-induced	1216:1226	arg1	phosphorylation					1228:1242	the LPS-induced phosphorylation	1212:1242	the LPS-induced phosphorylation of proteins related to the NF-κB and MAPK pathways in LPS-stimulated RAW264.7 cells	1212:1326	In addition, the A. personatus lipid suppressed the LPS-induced phosphorylation of proteins related to the NF-κB and MAPK pathways in LPS-stimulated RAW264.7 cells.
34641571	4	26	theme	A.	505:506	arg1	lipid					519:523	the A. personatus lipid	501:523	the A. personatus lipid	501:523	The immunomodulatory activities of the A. personatus lipid were investigated using rodent macrophages.
34641571	10	27	theme	egg	1488:1490	arg1	source					1513:1518	a potential source	1501:1518	a potential source of immunomodulation	1501:1538	The results indicate that A. personatus egg lipid is a potential source of immunomodulation.
34641571	10	27	theme	egg	1488:1490	arg1	lipid					1492:1496	A. personatus egg lipid	1474:1496	A. personatus egg lipid	1474:1496	The results indicate that A. personatus egg lipid is a potential source of immunomodulation.
34641571	4	28	dep	A.	505:506	arg1	personatus					508:517	personatus	508:517	personatus	508:517	The immunomodulatory activities of the A. personatus lipid were investigated using rodent macrophages.
34641571	2	29	theme	ionization	331:340	arg1	detection					342:350	-flame ionization detection	324:350	gas chromatography (GC)-flame ionization detection (FID)	301:356	In this study, the total lipid was extracted from A. personatus eggs and the fatty acid composition was determined using gas chromatography (GC)-flame ionization detection (FID).
34641571	2	29	theme	ionization	331:340	arg1	FID					353:355	FID	353:355	FID	353:355	In this study, the total lipid was extracted from A. personatus eggs and the fatty acid composition was determined using gas chromatography (GC)-flame ionization detection (FID).
34641571	0	30	theme	Immunomodulatory	0:15	arg1	Activities					17:26	Immunomodulatory Activities	0:26	Immunomodulatory Activities of Ammodytes personatus Egg Lipid in RAW264.7 Cells	0:78	Immunomodulatory Activities of Ammodytes personatus Egg Lipid in RAW264.7 Cells.
34641571	7	31	theme	NO	1069:1070	arg1	production					1072:1081	NO production	1069:1081	NO production	1069:1081	Conversely, the anti-inflammatory activity of the A. personatus lipid was also analyzed and the results showed significantly decreased NO production and gene expression in a dose-dependent manner in LPS-stimulated RAW264.7 cells.
34641571	2	32	theme	acid	263:266	arg1	composition					268:278	the fatty acid composition	253:278	the fatty acid composition	253:278	In this study, the total lipid was extracted from A. personatus eggs and the fatty acid composition was determined using gas chromatography (GC)-flame ionization detection (FID).
34641571	8	33	theme	related	1256:1262	arg1	proteins					1247:1254	proteins	1247:1254	proteins related to the NF-κB and MAPK pathways in LPS-stimulated RAW264.7 cells	1247:1326	In addition, the A. personatus lipid suppressed the LPS-induced phosphorylation of proteins related to the NF-κB and MAPK pathways in LPS-stimulated RAW264.7 cells.
34641571	2	34	theme	-flame	324:329	arg1	detection					342:350	-flame ionization detection	324:350	gas chromatography (GC)-flame ionization detection (FID)	301:356	In this study, the total lipid was extracted from A. personatus eggs and the fatty acid composition was determined using gas chromatography (GC)-flame ionization detection (FID).
34641571	2	34	theme	-flame	324:329	arg1	FID					353:355	FID	353:355	FID	353:355	In this study, the total lipid was extracted from A. personatus eggs and the fatty acid composition was determined using gas chromatography (GC)-flame ionization detection (FID).
34641571	0	35	theme	Ammodytes	31:39	arg1	Lipid					56:60	Ammodytes personatus Egg Lipid	31:60	Ammodytes personatus Egg Lipid	31:60	Immunomodulatory Activities of Ammodytes personatus Egg Lipid in RAW264.7 Cells.
34641571	2	36	theme	fatty	257:261	arg1	composition					268:278	the fatty acid composition	253:278	the fatty acid composition	253:278	In this study, the total lipid was extracted from A. personatus eggs and the fatty acid composition was determined using gas chromatography (GC)-flame ionization detection (FID).
34641571	9	37	theme	anti-inflammatory	1386:1402	arg1	activity					1404:1411	the lipid-regulated anti-inflammatory activity	1366:1411	the lipid-regulated anti-inflammatory activity	1366:1411	Further, flow cytometry demonstrated the lipid-regulated anti-inflammatory activity via inhibition of CD86 expression.
34641571	1	38	theme	Pacific	171:177	arg1	areas					152:156	cold areas	147:156	cold areas of the North Pacific	147:177	Ammodytes personatus, known as the Pacific sand lance, thrives in cold areas of the North Pacific.
34641571	5	39	theme	genes	788:792	arg1	transcription					753:765	the transcription	749:765	the transcription of immune-associated genes in RAW264.7 cells	749:810	First, immune enhancement was analyzed, and the A. personatus lipid significantly and dose-dependently increased the NO production in RAW264.7 cells, and this lipid also regulated the transcription of immune-associated genes in RAW264.7 cells by activating the NF-κB and MAPK pathways.
34641571	9	40	theme	flow	1338:1341	arg1	cytometry					1343:1351	flow cytometry	1338:1351	flow cytometry	1338:1351	Further, flow cytometry demonstrated the lipid-regulated anti-inflammatory activity via inhibition of CD86 expression.
34641571	5	41	from	production	689:698	arg1	cells					712:716	RAW264.7 cells	703:716	RAW264.7 cells	703:716	First, immune enhancement was analyzed, and the A. personatus lipid significantly and dose-dependently increased the NO production in RAW264.7 cells, and this lipid also regulated the transcription of immune-associated genes in RAW264.7 cells by activating the NF-κB and MAPK pathways.
34641571	3	42	theme	high	413:416	arg1	content					418:424	high content	413:424	high content of polyunsaturated fatty acids (PUFAs)	413:463	The results showed that the extracted lipid contained high content of polyunsaturated fatty acids (PUFAs).
34641571	0	43	theme	Egg	52:54	arg1	Lipid					56:60	Ammodytes personatus Egg Lipid	31:60	Ammodytes personatus Egg Lipid	31:60	Immunomodulatory Activities of Ammodytes personatus Egg Lipid in RAW264.7 Cells.
34641571	3	44	theme	fatty	445:449	arg1	PUFAs					458:462	PUFAs	458:462	PUFAs	458:462	The results showed that the extracted lipid contained high content of polyunsaturated fatty acids (PUFAs).
34641571	3	44	theme	fatty	445:449	arg1	acids					451:455	polyunsaturated fatty acids	429:455	polyunsaturated fatty acids (PUFAs)	429:463	The results showed that the extracted lipid contained high content of polyunsaturated fatty acids (PUFAs).
34641571	10	45	theme	potential	1503:1511	arg1	source					1513:1518	a potential source	1501:1518	a potential source of immunomodulation	1501:1538	The results indicate that A. personatus egg lipid is a potential source of immunomodulation.
34641571	10	45	theme	potential	1503:1511	arg1	lipid					1492:1496	A. personatus egg lipid	1474:1496	A. personatus egg lipid	1474:1496	The results indicate that A. personatus egg lipid is a potential source of immunomodulation.
34641571	7	46	dep	A.	984:985	arg1	personatus					987:996	personatus	987:996	personatus	987:996	Conversely, the anti-inflammatory activity of the A. personatus lipid was also analyzed and the results showed significantly decreased NO production and gene expression in a dose-dependent manner in LPS-stimulated RAW264.7 cells.
34641571	2	47	theme	total	199:203	arg1	lipid					205:209	the total lipid	195:209	the total lipid	195:209	In this study, the total lipid was extracted from A. personatus eggs and the fatty acid composition was determined using gas chromatography (GC)-flame ionization detection (FID).
34641571	3	48	theme	extracted	387:395	arg1	lipid					397:401	the extracted lipid	383:401	the extracted lipid	383:401	The results showed that the extracted lipid contained high content of polyunsaturated fatty acids (PUFAs).
34641571	0	49	theme	personatus	41:50	arg1	Lipid					56:60	Ammodytes personatus Egg Lipid	31:60	Ammodytes personatus Egg Lipid	31:60	Immunomodulatory Activities of Ammodytes personatus Egg Lipid in RAW264.7 Cells.
34641571	7	50	theme	LPS-stimulated	1133:1146	arg1	cells					1157:1161	LPS-stimulated RAW264.7 cells	1133:1161	LPS-stimulated RAW264.7 cells	1133:1161	Conversely, the anti-inflammatory activity of the A. personatus lipid was also analyzed and the results showed significantly decreased NO production and gene expression in a dose-dependent manner in LPS-stimulated RAW264.7 cells.
34641571	8	51	theme	NF-κB	1271:1275	arg1	pathways					1286:1293	the NF-κB and MAPK pathways	1267:1293	pathways	1286:1293	In addition, the A. personatus lipid suppressed the LPS-induced phosphorylation of proteins related to the NF-κB and MAPK pathways in LPS-stimulated RAW264.7 cells.
34641571	5	52	theme	immune-associated	770:786	arg1	genes					788:792	immune-associated genes	770:792	immune-associated genes	770:792	First, immune enhancement was analyzed, and the A. personatus lipid significantly and dose-dependently increased the NO production in RAW264.7 cells, and this lipid also regulated the transcription of immune-associated genes in RAW264.7 cells by activating the NF-κB and MAPK pathways.
34641571	7	53	theme	RAW264.7	1148:1155	arg1	cells					1157:1161	LPS-stimulated RAW264.7 cells	1133:1161	LPS-stimulated RAW264.7 cells	1133:1161	Conversely, the anti-inflammatory activity of the A. personatus lipid was also analyzed and the results showed significantly decreased NO production and gene expression in a dose-dependent manner in LPS-stimulated RAW264.7 cells.
34641571	7	54	theme	dose-dependent	1108:1121	arg1	manner					1123:1128	a dose-dependent manner	1106:1128	a dose-dependent manner in LPS-stimulated RAW264.7 cells	1106:1161	Conversely, the anti-inflammatory activity of the A. personatus lipid was also analyzed and the results showed significantly decreased NO production and gene expression in a dose-dependent manner in LPS-stimulated RAW264.7 cells.
34641571	5	55	theme	A.	617:618	arg1	lipid					631:635	the A. personatus lipid	613:635	the A. personatus lipid	613:635	First, immune enhancement was analyzed, and the A. personatus lipid significantly and dose-dependently increased the NO production in RAW264.7 cells, and this lipid also regulated the transcription of immune-associated genes in RAW264.7 cells by activating the NF-κB and MAPK pathways.
34641571	3	56	contain	contained	403:411	arg1	lipid					397:401	the extracted lipid	383:401	the extracted lipid	383:401	The results showed that the extracted lipid contained high content of polyunsaturated fatty acids (PUFAs).
34641571	3	56	contain	contained	403:411	arg2	content					418:424	high content	413:424	high content of polyunsaturated fatty acids (PUFAs)	413:463	The results showed that the extracted lipid contained high content of polyunsaturated fatty acids (PUFAs).
34641571	7	57	theme	lipid	998:1002	arg1	activity					968:975	the anti-inflammatory activity	946:975	the anti-inflammatory activity of the A. personatus lipid	946:1002	Conversely, the anti-inflammatory activity of the A. personatus lipid was also analyzed and the results showed significantly decreased NO production and gene expression in a dose-dependent manner in LPS-stimulated RAW264.7 cells.
34641571	2	58	theme	gas	301:303	arg1	GC					321:322	GC	321:322	GC	321:322	In this study, the total lipid was extracted from A. personatus eggs and the fatty acid composition was determined using gas chromatography (GC)-flame ionization detection (FID).
34641571	2	58	theme	gas	301:303	arg1	chromatography					305:318	gas chromatography	301:318	gas chromatography (GC)-flame ionization detection (FID)	301:356	In this study, the total lipid was extracted from A. personatus eggs and the fatty acid composition was determined using gas chromatography (GC)-flame ionization detection (FID).
34641571	0	59	theme	Lipid	56:60	arg1	Activities					17:26	Immunomodulatory Activities	0:26	Immunomodulatory Activities of Ammodytes personatus Egg Lipid in RAW264.7 Cells	0:78	Immunomodulatory Activities of Ammodytes personatus Egg Lipid in RAW264.7 Cells.
34641571	9	60	theme	lipid-regulated	1370:1384	arg1	activity					1404:1411	the lipid-regulated anti-inflammatory activity	1366:1411	the lipid-regulated anti-inflammatory activity	1366:1411	Further, flow cytometry demonstrated the lipid-regulated anti-inflammatory activity via inhibition of CD86 expression.
34641571	6	61	theme	flow	869:872	arg1	cytometry					874:882	flow cytometry	869:882	flow cytometry	869:882	Additionally, flow cytometry revealed that this lipid stimulated phagocytosis.
34641571	1	62	theme	Pacific	116:122	arg1	lance					129:133	the Pacific sand lance	112:133	the Pacific sand lance	112:133	Ammodytes personatus, known as the Pacific sand lance, thrives in cold areas of the North Pacific.
34641571	4	63	theme	immunomodulatory	470:485	arg1	activities					487:496	The immunomodulatory activities	466:496	The immunomodulatory activities of the A. personatus lipid	466:523	The immunomodulatory activities of the A. personatus lipid were investigated using rodent macrophages.
34641571	7	64	theme	A.	984:985	arg1	lipid					998:1002	the A. personatus lipid	980:1002	the A. personatus lipid	980:1002	Conversely, the anti-inflammatory activity of the A. personatus lipid was also analyzed and the results showed significantly decreased NO production and gene expression in a dose-dependent manner in LPS-stimulated RAW264.7 cells.
34641571	1	65	theme	sand	124:127	arg1	lance					129:133	the Pacific sand lance	112:133	the Pacific sand lance	112:133	Ammodytes personatus, known as the Pacific sand lance, thrives in cold areas of the North Pacific.
34641571	10	66	theme	immunomodulation	1523:1538	arg1	source					1513:1518	a potential source	1501:1518	a potential source of immunomodulation	1501:1538	The results indicate that A. personatus egg lipid is a potential source of immunomodulation.
34641571	10	66	theme	immunomodulation	1523:1538	arg1	lipid					1492:1496	A. personatus egg lipid	1474:1496	A. personatus egg lipid	1474:1496	The results indicate that A. personatus egg lipid is a potential source of immunomodulation.
34641571	9	67	theme	expression	1436:1445	arg1	inhibition					1417:1426	inhibition	1417:1426	inhibition of CD86 expression	1417:1445	Further, flow cytometry demonstrated the lipid-regulated anti-inflammatory activity via inhibition of CD86 expression.
34641571	8	68	theme	RAW264.7	1313:1320	arg1	cells					1322:1326	LPS-stimulated RAW264.7 cells	1298:1326	LPS-stimulated RAW264.7 cells	1298:1326	In addition, the A. personatus lipid suppressed the LPS-induced phosphorylation of proteins related to the NF-κB and MAPK pathways in LPS-stimulated RAW264.7 cells.
34641571	8	69	theme	LPS-stimulated	1298:1311	arg1	cells					1322:1326	LPS-stimulated RAW264.7 cells	1298:1326	LPS-stimulated RAW264.7 cells	1298:1326	In addition, the A. personatus lipid suppressed the LPS-induced phosphorylation of proteins related to the NF-κB and MAPK pathways in LPS-stimulated RAW264.7 cells.
34641571	10	70	dep	A.	1474:1475	arg1	personatus					1477:1486	personatus	1477:1486	personatus	1477:1486	The results indicate that A. personatus egg lipid is a potential source of immunomodulation.
34641571	4	71	theme	rodent	549:554	arg1	macrophages					556:566	rodent macrophages	549:566	rodent macrophages	549:566	The immunomodulatory activities of the A. personatus lipid were investigated using rodent macrophages.
34641571	5	72	theme	MAPK	840:843	arg1	pathways					845:852	the NF-κB and MAPK pathways	826:852	pathways	845:852	First, immune enhancement was analyzed, and the A. personatus lipid significantly and dose-dependently increased the NO production in RAW264.7 cells, and this lipid also regulated the transcription of immune-associated genes in RAW264.7 cells by activating the NF-κB and MAPK pathways.
34641571	0	73	theme	RAW264.7	65:72	arg1	Cells					74:78	RAW264.7 Cells	65:78	RAW264.7 Cells	65:78	Immunomodulatory Activities of Ammodytes personatus Egg Lipid in RAW264.7 Cells.
34641571	2	74	dep	A.	230:231	arg1	personatus					233:242	personatus	233:242	personatus	233:242	In this study, the total lipid was extracted from A. personatus eggs and the fatty acid composition was determined using gas chromatography (GC)-flame ionization detection (FID).
32910752	6	0	theme	diamino	880:886	arg1	acid					888:891	a diagnostic diamino acid	867:891	a diagnostic diamino acid	867:891	The cell-wall peptidoglycan was meso-diaminopimelic acid as a diagnostic diamino acid.
32910752	13	1	from	Nocardiaceae	1601:1612	arg1	species					1564:1570	a novel species	1556:1570	a novel species of a new genus in the family Nocardiaceae	1556:1612	On the basis of combination of morphological and chemotaxonomic differences, in addition to phylogenetic distinctness, the novel isolates are considered to constitute members of a novel species of a new genus in the family Nocardiaceae, for which the name Antrihabitans stalactiti gen. nov., sp.
32910752	4	2	theme	gene-based	533:542	arg1	analysis					557:564	the 92 core gene-based phylogenomic analysis	521:564	the 92 core gene-based phylogenomic analysis	521:564	In the 92 core gene-based phylogenomic analysis, strain YC2-7T was loosely associated with the type strain of Aldersonia kummingensis with 66.2 % average amino acid identity.
32910752	13	3	dep	combination	1394:1404	arg1	basis					1385:1389	basis	1385:1389	basis	1385:1389	On the basis of combination of morphological and chemotaxonomic differences, in addition to phylogenetic distinctness, the novel isolates are considered to constitute members of a novel species of a new genus in the family Nocardiaceae, for which the name Antrihabitans stalactiti gen. nov., sp.
32910752	13	3	dep	combination	1394:1404	arg1	the					1381:1383	the	1381:1383	the	1381:1383	On the basis of combination of morphological and chemotaxonomic differences, in addition to phylogenetic distinctness, the novel isolates are considered to constitute members of a novel species of a new genus in the family Nocardiaceae, for which the name Antrihabitans stalactiti gen. nov., sp.
32910752	13	4	theme	chemotaxonomic	1427:1440	arg1	differences					1442:1452	morphological and chemotaxonomic differences	1409:1452	morphological and chemotaxonomic differences	1409:1452	On the basis of combination of morphological and chemotaxonomic differences, in addition to phylogenetic distinctness, the novel isolates are considered to constitute members of a novel species of a new genus in the family Nocardiaceae, for which the name Antrihabitans stalactiti gen. nov., sp.
32910752	10	5	theme	unsaturated	1211:1221	arg1	components					1223:1232	saturated and unsaturated components	1197:1232	saturated and unsaturated components	1197:1232	The cellular fatty acids consisted mainly of saturated and unsaturated components with small amounts of tuberculostearic acid.
32910752	3	6	theme	Nocardiaceae	504:515	arg1	genera					483:488	recognized genera	472:488	recognized genera of the family Nocardiaceae	472:515	The 16S rRNA gene tree showed that the novel isolates occupied an independent position separated from recognized genera of the family Nocardiaceae.
32910752	10	7	theme	saturated	1197:1205	arg1	components					1223:1232	saturated and unsaturated components	1197:1232	saturated and unsaturated components	1197:1232	The cellular fatty acids consisted mainly of saturated and unsaturated components with small amounts of tuberculostearic acid.
32910752	8	8	theme	predominant	963:973	arg1	menaquinone					975:985	The predominant menaquinone	959:985	The predominant menaquinone	959:985	The predominant menaquinone was MK-8(H4, ω-cycl).
32910752	8	8	theme	predominant	963:973	arg1	MK-8					991:994	MK-8	991:994	MK-8	991:994	The predominant menaquinone was MK-8(H4, ω-cycl).
32910752	4	9	theme	Aldersonia	628:637	arg1	kummingensis					639:650	Aldersonia kummingensis	628:650	Aldersonia kummingensis	628:650	In the 92 core gene-based phylogenomic analysis, strain YC2-7T was loosely associated with the type strain of Aldersonia kummingensis with 66.2 % average amino acid identity.
32910752	13	10	from	species	1564:1570	arg1	Nocardiaceae					1601:1612	the family Nocardiaceae	1590:1612	the family Nocardiaceae	1590:1612	On the basis of combination of morphological and chemotaxonomic differences, in addition to phylogenetic distinctness, the novel isolates are considered to constitute members of a novel species of a new genus in the family Nocardiaceae, for which the name Antrihabitans stalactiti gen. nov., sp.
32910752	13	11	theme	genus	1581:1585	arg1	species					1564:1570	a novel species	1556:1570	a novel species of a new genus in the family Nocardiaceae	1556:1612	On the basis of combination of morphological and chemotaxonomic differences, in addition to phylogenetic distinctness, the novel isolates are considered to constitute members of a novel species of a new genus in the family Nocardiaceae, for which the name Antrihabitans stalactiti gen. nov., sp.
32910752	13	12	dep	sp	1670:1671	arg1	nov.					1664:1667	the name Antrihabitans stalactiti gen. nov.	1625:1667	the name Antrihabitans stalactiti gen. nov.	1625:1667	On the basis of combination of morphological and chemotaxonomic differences, in addition to phylogenetic distinctness, the novel isolates are considered to constitute members of a novel species of a new genus in the family Nocardiaceae, for which the name Antrihabitans stalactiti gen. nov., sp.
32910752	4	13	theme	amino	672:676	arg1	identity					683:690	66.2 % average amino acid identity	657:690	66.2 % average amino acid identity	657:690	In the 92 core gene-based phylogenomic analysis, strain YC2-7T was loosely associated with the type strain of Aldersonia kummingensis with 66.2 % average amino acid identity.
32910752	13	14	theme	species	1564:1570	arg1	members					1545:1551	members	1545:1551	members	1545:1551	On the basis of combination of morphological and chemotaxonomic differences, in addition to phylogenetic distinctness, the novel isolates are considered to constitute members of a novel species of a new genus in the family Nocardiaceae, for which the name Antrihabitans stalactiti gen. nov., sp.
32910752	12	15	theme	DNA	1333:1335	arg1	content					1341:1347	The DNA G+C content	1329:1347	The DNA G+C content of the genome	1329:1361	The DNA G+C content of the genome was 63.8 mol%.
32910752	12	15	theme	DNA	1333:1335	arg1	%					1375:1375	63.8 mol%	1367:1375	63.8 mol%	1367:1375	The DNA G+C content of the genome was 63.8 mol%.
32910752	15	16	theme	19965T=DSM	1725:1734	arg1	108733T					1736:1742	=KACC 19965T=DSM 108733T	1719:1742	=KACC 19965T=DSM 108733T	1719:1742	The type strain is YC2-7T (=KACC 19965T=DSM 108733T).
32910752	15	16	theme	19965T=DSM	1725:1734	arg1	YC2-7T					1711:1716	YC2-7T	1711:1716	YC2-7T (=KACC 19965T=DSM 108733T)	1711:1743	The type strain is YC2-7T (=KACC 19965T=DSM 108733T).
32910752	2	17	theme	cave	271:274	arg1	larva					265:269	larva cave	265:274	larva cave	265:274	Two Gram-stain-positive, strictly aerobic, non-spore-forming actinobacterial strains, designated YC2-7T and YC5-17, were isolated from the Yongcheondonggul (larva cave) in Jeju, Republic of Korea and their taxonomic ranks were examined by a polyphasic approach.
32910752	2	17	theme	cave	271:274	arg1	Yongcheondonggul					247:262	the Yongcheondonggul	243:262	the Yongcheondonggul (larva cave) in Jeju, Republic of Korea	243:302	Two Gram-stain-positive, strictly aerobic, non-spore-forming actinobacterial strains, designated YC2-7T and YC5-17, were isolated from the Yongcheondonggul (larva cave) in Jeju, Republic of Korea and their taxonomic ranks were examined by a polyphasic approach.
32910752	5	18	theme	sequence	711:718	arg1	simairity					720:728	The 16S rRNA gene sequence simairity	693:728	The 16S rRNA gene sequence simairity between the isolate	693:748	The 16S rRNA gene sequence simairity between the isolate and members of the family Nocardiaceae was below 96.7 %.
32910752	5	19	theme	Nocardiaceae	776:787	arg1	members					754:760	members	754:760	members of the family Nocardiaceae	754:787	The 16S rRNA gene sequence simairity between the isolate and members of the family Nocardiaceae was below 96.7 %.
32910752	5	19	theme	Nocardiaceae	776:787	arg1	simairity					720:728	The 16S rRNA gene sequence simairity	693:728	The 16S rRNA gene sequence simairity between the isolate	693:748	The 16S rRNA gene sequence simairity between the isolate and members of the family Nocardiaceae was below 96.7 %.
32910752	2	20	attach	isolated	229:236	arg1	larva					265:269	larva cave	265:274	larva cave	265:274	Two Gram-stain-positive, strictly aerobic, non-spore-forming actinobacterial strains, designated YC2-7T and YC5-17, were isolated from the Yongcheondonggul (larva cave) in Jeju, Republic of Korea and their taxonomic ranks were examined by a polyphasic approach.
32910752	2	20	attach	isolated	229:236	arg2	strains					185:191	Two Gram-stain-positive, strictly aerobic, non-spore-forming actinobacterial strains	108:191	Two Gram-stain-positive, strictly aerobic, non-spore-forming actinobacterial strains	108:191	Two Gram-stain-positive, strictly aerobic, non-spore-forming actinobacterial strains, designated YC2-7T and YC5-17, were isolated from the Yongcheondonggul (larva cave) in Jeju, Republic of Korea and their taxonomic ranks were examined by a polyphasic approach.
32910752	2	20	attach	isolated	229:236	arg1	Yongcheondonggul					247:262	the Yongcheondonggul	243:262	the Yongcheondonggul (larva cave) in Jeju, Republic of Korea	243:302	Two Gram-stain-positive, strictly aerobic, non-spore-forming actinobacterial strains, designated YC2-7T and YC5-17, were isolated from the Yongcheondonggul (larva cave) in Jeju, Republic of Korea and their taxonomic ranks were examined by a polyphasic approach.
32910752	4	21	theme	%	662:662	arg1	identity					683:690	66.2 % average amino acid identity	657:690	66.2 % average amino acid identity	657:690	In the 92 core gene-based phylogenomic analysis, strain YC2-7T was loosely associated with the type strain of Aldersonia kummingensis with 66.2 % average amino acid identity.
32910752	9	22	theme	major	1013:1017	arg1	lipids					1025:1030	The major polar lipids	1009:1030	The major polar lipids	1009:1030	The major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside.
32910752	2	23	theme	polyphasic	349:358	arg1	approach					360:367	a polyphasic approach	347:367	a polyphasic approach	347:367	Two Gram-stain-positive, strictly aerobic, non-spore-forming actinobacterial strains, designated YC2-7T and YC5-17, were isolated from the Yongcheondonggul (larva cave) in Jeju, Republic of Korea and their taxonomic ranks were examined by a polyphasic approach.
32910752	5	24	theme	rRNA	701:704	arg1	simairity					720:728	The 16S rRNA gene sequence simairity	693:728	The 16S rRNA gene sequence simairity between the isolate	693:748	The 16S rRNA gene sequence simairity between the isolate and members of the family Nocardiaceae was below 96.7 %.
32910752	11	25	theme	atoms	1309:1313	arg1	acids					1287:1291	Mycolic acids	1279:1291	Mycolic acids of 52-58 carbon atoms	1279:1313	Mycolic acids of 52-58 carbon atoms were present.
32910752	3	26	theme	rRNA	378:381	arg1	tree					388:391	The 16S rRNA gene tree	370:391	The 16S rRNA gene tree	370:391	The 16S rRNA gene tree showed that the novel isolates occupied an independent position separated from recognized genera of the family Nocardiaceae.
32910752	0	27	theme	Antrihabitans	0:12	arg1	nov.					30:33	Antrihabitans stalactiti gen. nov.	0:33	Antrihabitans stalactiti gen. nov.	0:33	Antrihabitans stalactiti gen. nov., sp.
32910752	13	28	theme	name	1629:1632	arg1	nov.					1664:1667	the name Antrihabitans stalactiti gen. nov.	1625:1667	the name Antrihabitans stalactiti gen. nov.	1625:1667	On the basis of combination of morphological and chemotaxonomic differences, in addition to phylogenetic distinctness, the novel isolates are considered to constitute members of a novel species of a new genus in the family Nocardiaceae, for which the name Antrihabitans stalactiti gen. nov., sp.
32910752	3	29	theme	novel	409:413	arg1	isolates					415:422	the novel isolates	405:422	the novel isolates	405:422	The 16S rRNA gene tree showed that the novel isolates occupied an independent position separated from recognized genera of the family Nocardiaceae.
32910752	0	30	theme	gen.	25:28	arg1	nov.					30:33	Antrihabitans stalactiti gen. nov.	0:33	Antrihabitans stalactiti gen. nov.	0:33	Antrihabitans stalactiti gen. nov., sp.
32910752	1	31	theme	Nocardiaceae	73:84	arg1	member					52:57	a new member	46:57	a new member of the family Nocardiaceae isolated from a cave	46:105	nov., a new member of the family Nocardiaceae isolated from a cave.
32910752	1	31	theme	Nocardiaceae	73:84	arg1	nov.					40:43	nov.	40:43	nov.	40:43	nov., a new member of the family Nocardiaceae isolated from a cave.
32910752	15	32	theme	type	1696:1699	arg1	strain					1701:1706	The type strain	1692:1706	The type strain	1692:1706	The type strain is YC2-7T (=KACC 19965T=DSM 108733T).
32910752	15	32	theme	type	1696:1699	arg1	YC2-7T					1711:1716	YC2-7T	1711:1716	YC2-7T (=KACC 19965T=DSM 108733T)	1711:1743	The type strain is YC2-7T (=KACC 19965T=DSM 108733T).
32910752	2	33	dep	Gram-stain-positive	112:130	arg1	non-spore-forming					151:167	non-spore-forming	151:167	non-spore-forming	151:167	Two Gram-stain-positive, strictly aerobic, non-spore-forming actinobacterial strains, designated YC2-7T and YC5-17, were isolated from the Yongcheondonggul (larva cave) in Jeju, Republic of Korea and their taxonomic ranks were examined by a polyphasic approach.
32910752	2	33	dep	Gram-stain-positive	112:130	arg1	aerobic					142:148	aerobic	142:148	aerobic	142:148	Two Gram-stain-positive, strictly aerobic, non-spore-forming actinobacterial strains, designated YC2-7T and YC5-17, were isolated from the Yongcheondonggul (larva cave) in Jeju, Republic of Korea and their taxonomic ranks were examined by a polyphasic approach.
32910752	13	34	theme	stalactiti	1648:1657	arg1	nov.					1664:1667	the name Antrihabitans stalactiti gen. nov.	1625:1667	the name Antrihabitans stalactiti gen. nov.	1625:1667	On the basis of combination of morphological and chemotaxonomic differences, in addition to phylogenetic distinctness, the novel isolates are considered to constitute members of a novel species of a new genus in the family Nocardiaceae, for which the name Antrihabitans stalactiti gen. nov., sp.
32910752	10	35	theme	fatty	1165:1169	arg1	acids					1171:1175	The cellular fatty acids	1152:1175	The cellular fatty acids	1152:1175	The cellular fatty acids consisted mainly of saturated and unsaturated components with small amounts of tuberculostearic acid.
32910752	13	36	theme	differences	1442:1452	arg1	combination					1394:1404	combination	1394:1404	combination of morphological and chemotaxonomic differences	1394:1452	On the basis of combination of morphological and chemotaxonomic differences, in addition to phylogenetic distinctness, the novel isolates are considered to constitute members of a novel species of a new genus in the family Nocardiaceae, for which the name Antrihabitans stalactiti gen. nov., sp.
32910752	2	37	theme	actinobacterial	169:183	arg1	strains					185:191	Two Gram-stain-positive, strictly aerobic, non-spore-forming actinobacterial strains	108:191	Two Gram-stain-positive, strictly aerobic, non-spore-forming actinobacterial strains	108:191	Two Gram-stain-positive, strictly aerobic, non-spore-forming actinobacterial strains, designated YC2-7T and YC5-17, were isolated from the Yongcheondonggul (larva cave) in Jeju, Republic of Korea and their taxonomic ranks were examined by a polyphasic approach.
32910752	3	38	theme	recognized	472:481	arg1	genera					483:488	recognized genera	472:488	recognized genera of the family Nocardiaceae	472:515	The 16S rRNA gene tree showed that the novel isolates occupied an independent position separated from recognized genera of the family Nocardiaceae.
32910752	9	39	dep	diphosphatidylglycerol	1045:1066	arg1	mannoside					1141:1149	mannoside	1141:1149	mannoside	1141:1149	The major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside.
32910752	1	40	attach	isolated	86:93	arg2	Nocardiaceae					73:84	the family Nocardiaceae	62:84	the family Nocardiaceae isolated from a cave	62:105	nov., a new member of the family Nocardiaceae isolated from a cave.
32910752	1	40	attach	isolated	86:93	arg1	cave					102:105	a cave	100:105	a cave	100:105	nov., a new member of the family Nocardiaceae isolated from a cave.
32910752	2	41	theme	taxonomic	314:322	arg1	ranks					324:328	their taxonomic ranks	308:328	their taxonomic ranks	308:328	Two Gram-stain-positive, strictly aerobic, non-spore-forming actinobacterial strains, designated YC2-7T and YC5-17, were isolated from the Yongcheondonggul (larva cave) in Jeju, Republic of Korea and their taxonomic ranks were examined by a polyphasic approach.
32910752	6	42	theme	diagnostic	869:878	arg1	acid					888:891	a diagnostic diamino acid	867:891	a diagnostic diamino acid	867:891	The cell-wall peptidoglycan was meso-diaminopimelic acid as a diagnostic diamino acid.
32910752	4	43	theme	core	528:531	arg1	analysis					557:564	the 92 core gene-based phylogenomic analysis	521:564	the 92 core gene-based phylogenomic analysis	521:564	In the 92 core gene-based phylogenomic analysis, strain YC2-7T was loosely associated with the type strain of Aldersonia kummingensis with 66.2 % average amino acid identity.
32910752	3	44	theme	family	497:502	arg1	Nocardiaceae					504:515	the family Nocardiaceae	493:515	the family Nocardiaceae	493:515	The 16S rRNA gene tree showed that the novel isolates occupied an independent position separated from recognized genera of the family Nocardiaceae.
32910752	4	45	theme	phylogenomic	544:555	arg1	analysis					557:564	the 92 core gene-based phylogenomic analysis	521:564	the 92 core gene-based phylogenomic analysis	521:564	In the 92 core gene-based phylogenomic analysis, strain YC2-7T was loosely associated with the type strain of Aldersonia kummingensis with 66.2 % average amino acid identity.
32910752	2	46	theme	Gram-stain-positive	112:130	arg1	strains					185:191	Two Gram-stain-positive, strictly aerobic, non-spore-forming actinobacterial strains	108:191	Two Gram-stain-positive, strictly aerobic, non-spore-forming actinobacterial strains	108:191	Two Gram-stain-positive, strictly aerobic, non-spore-forming actinobacterial strains, designated YC2-7T and YC5-17, were isolated from the Yongcheondonggul (larva cave) in Jeju, Republic of Korea and their taxonomic ranks were examined by a polyphasic approach.
32910752	13	47	theme	family	1594:1599	arg1	Nocardiaceae					1601:1612	the family Nocardiaceae	1590:1612	the family Nocardiaceae	1590:1612	On the basis of combination of morphological and chemotaxonomic differences, in addition to phylogenetic distinctness, the novel isolates are considered to constitute members of a novel species of a new genus in the family Nocardiaceae, for which the name Antrihabitans stalactiti gen. nov., sp.
32910752	6	48	theme	meso-diaminopimelic	839:857	arg1	acid					859:862	meso-diaminopimelic acid	839:862	meso-diaminopimelic acid	839:862	The cell-wall peptidoglycan was meso-diaminopimelic acid as a diagnostic diamino acid.
32910752	6	48	theme	meso-diaminopimelic	839:857	arg1	peptidoglycan					821:833	The cell-wall peptidoglycan	807:833	The cell-wall peptidoglycan	807:833	The cell-wall peptidoglycan was meso-diaminopimelic acid as a diagnostic diamino acid.
32910752	4	49	theme	type	613:616	arg1	strain					618:623	the type strain	609:623	the type strain of Aldersonia kummingensis with 66.2 % average amino acid identity	609:690	In the 92 core gene-based phylogenomic analysis, strain YC2-7T was loosely associated with the type strain of Aldersonia kummingensis with 66.2 % average amino acid identity.
32910752	12	50	theme	63.8 mol	1367:1374	arg1	content					1341:1347	The DNA G+C content	1329:1347	The DNA G+C content of the genome	1329:1361	The DNA G+C content of the genome was 63.8 mol%.
32910752	12	50	theme	63.8 mol	1367:1374	arg1	%					1375:1375	63.8 mol%	1367:1375	63.8 mol%	1367:1375	The DNA G+C content of the genome was 63.8 mol%.
32910752	13	51	theme	new	1577:1579	arg1	genus					1581:1585	a new genus	1575:1585	a new genus in the family Nocardiaceae	1575:1612	On the basis of combination of morphological and chemotaxonomic differences, in addition to phylogenetic distinctness, the novel isolates are considered to constitute members of a novel species of a new genus in the family Nocardiaceae, for which the name Antrihabitans stalactiti gen. nov., sp.
32910752	13	52	theme	novel	1558:1562	arg1	species					1564:1570	a novel species	1556:1570	a novel species of a new genus in the family Nocardiaceae	1556:1612	On the basis of combination of morphological and chemotaxonomic differences, in addition to phylogenetic distinctness, the novel isolates are considered to constitute members of a novel species of a new genus in the family Nocardiaceae, for which the name Antrihabitans stalactiti gen. nov., sp.
32910752	12	53	theme	genome	1356:1361	arg1	content					1341:1347	The DNA G+C content	1329:1347	The DNA G+C content of the genome	1329:1361	The DNA G+C content of the genome was 63.8 mol%.
32910752	12	53	theme	genome	1356:1361	arg1	%					1375:1375	63.8 mol%	1367:1375	63.8 mol%	1367:1375	The DNA G+C content of the genome was 63.8 mol%.
32910752	4	54	theme	average	664:670	arg1	identity					683:690	66.2 % average amino acid identity	657:690	66.2 % average amino acid identity	657:690	In the 92 core gene-based phylogenomic analysis, strain YC2-7T was loosely associated with the type strain of Aldersonia kummingensis with 66.2 % average amino acid identity.
32910752	15	55	theme	=KACC	1719:1723	arg1	108733T					1736:1742	=KACC 19965T=DSM 108733T	1719:1742	=KACC 19965T=DSM 108733T	1719:1742	The type strain is YC2-7T (=KACC 19965T=DSM 108733T).
32910752	15	55	theme	=KACC	1719:1723	arg1	YC2-7T					1711:1716	YC2-7T	1711:1716	YC2-7T (=KACC 19965T=DSM 108733T)	1711:1743	The type strain is YC2-7T (=KACC 19965T=DSM 108733T).
32910752	2	56	from	Yongcheondonggul	247:262	arg1	Republic					286:293	Republic	286:293	Republic	286:293	Two Gram-stain-positive, strictly aerobic, non-spore-forming actinobacterial strains, designated YC2-7T and YC5-17, were isolated from the Yongcheondonggul (larva cave) in Jeju, Republic of Korea and their taxonomic ranks were examined by a polyphasic approach.
32910752	4	57	theme	acid	678:681	arg1	identity					683:690	66.2 % average amino acid identity	657:690	66.2 % average amino acid identity	657:690	In the 92 core gene-based phylogenomic analysis, strain YC2-7T was loosely associated with the type strain of Aldersonia kummingensis with 66.2 % average amino acid identity.
32910752	10	58	theme	small	1239:1243	arg1	amounts					1245:1251	small amounts	1239:1251	small amounts of tuberculostearic acid	1239:1276	The cellular fatty acids consisted mainly of saturated and unsaturated components with small amounts of tuberculostearic acid.
32910752	10	58	theme	small	1239:1243	arg1	acid					1273:1276	tuberculostearic acid	1256:1276	tuberculostearic acid	1256:1276	The cellular fatty acids consisted mainly of saturated and unsaturated components with small amounts of tuberculostearic acid.
32910752	5	59	theme	gene	706:709	arg1	simairity					720:728	The 16S rRNA gene sequence simairity	693:728	The 16S rRNA gene sequence simairity between the isolate	693:748	The 16S rRNA gene sequence simairity between the isolate and members of the family Nocardiaceae was below 96.7 %.
32910752	10	60	theme	tuberculostearic	1256:1271	arg1	acid					1273:1276	tuberculostearic acid	1256:1276	tuberculostearic acid	1256:1276	The cellular fatty acids consisted mainly of saturated and unsaturated components with small amounts of tuberculostearic acid.
32910752	12	61	theme	G+C	1337:1339	arg1	content					1341:1347	The DNA G+C content	1329:1347	The DNA G+C content of the genome	1329:1361	The DNA G+C content of the genome was 63.8 mol%.
32910752	12	61	theme	G+C	1337:1339	arg1	%					1375:1375	63.8 mol%	1367:1375	63.8 mol%	1367:1375	The DNA G+C content of the genome was 63.8 mol%.
32910752	4	62	theme	kummingensis	639:650	arg1	strain					618:623	the type strain	609:623	the type strain of Aldersonia kummingensis with 66.2 % average amino acid identity	609:690	In the 92 core gene-based phylogenomic analysis, strain YC2-7T was loosely associated with the type strain of Aldersonia kummingensis with 66.2 % average amino acid identity.
32910752	11	63	theme	carbon	1302:1307	arg1	atoms					1309:1313	52-58 carbon atoms	1296:1313	52-58 carbon atoms	1296:1313	Mycolic acids of 52-58 carbon atoms were present.
32910752	4	64	theme	66.2 	657:661	arg1	%					662:662	%	662:662	%	662:662	In the 92 core gene-based phylogenomic analysis, strain YC2-7T was loosely associated with the type strain of Aldersonia kummingensis with 66.2 % average amino acid identity.
32910752	10	65	theme	acid	1273:1276	arg1	amounts					1245:1251	small amounts	1239:1251	small amounts of tuberculostearic acid	1239:1276	The cellular fatty acids consisted mainly of saturated and unsaturated components with small amounts of tuberculostearic acid.
32910752	10	65	theme	acid	1273:1276	arg1	acid					1273:1276	tuberculostearic acid	1256:1276	tuberculostearic acid	1256:1276	The cellular fatty acids consisted mainly of saturated and unsaturated components with small amounts of tuberculostearic acid.
32910752	8	66	dep	MK-8	991:994	arg1	ω-cycl					1000:1005	ω-cycl	1000:1005	ω-cycl	1000:1005	The predominant menaquinone was MK-8(H4, ω-cycl).
32910752	8	66	dep	MK-8	991:994	arg1	H4					996:997	H4	996:997	H4	996:997	The predominant menaquinone was MK-8(H4, ω-cycl).
32910752	5	67	theme	16S	697:699	arg1	rRNA					701:704	16S rRNA	697:704	The 16S rRNA gene sequence simairity between the isolate	693:748	The 16S rRNA gene sequence simairity between the isolate and members of the family Nocardiaceae was below 96.7 %.
32910752	9	68	theme	polar	1019:1023	arg1	lipids					1025:1030	The major polar lipids	1009:1030	The major polar lipids	1009:1030	The major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside.
32910752	1	69	theme	new	48:50	arg1	member					52:57	a new member	46:57	a new member of the family Nocardiaceae isolated from a cave	46:105	nov., a new member of the family Nocardiaceae isolated from a cave.
32910752	1	69	theme	new	48:50	arg1	nov.					40:43	nov.	40:43	nov.	40:43	nov., a new member of the family Nocardiaceae isolated from a cave.
32910752	3	70	theme	independent	436:446	arg1	position					448:455	an independent position	433:455	an independent position separated from recognized genera of the family Nocardiaceae	433:515	The 16S rRNA gene tree showed that the novel isolates occupied an independent position separated from recognized genera of the family Nocardiaceae.
32910752	13	71	theme	novel	1501:1505	arg1	isolates					1507:1514	the novel isolates	1497:1514	the novel isolates	1497:1514	On the basis of combination of morphological and chemotaxonomic differences, in addition to phylogenetic distinctness, the novel isolates are considered to constitute members of a novel species of a new genus in the family Nocardiaceae, for which the name Antrihabitans stalactiti gen. nov., sp.
32910752	0	72	theme	stalactiti	14:23	arg1	nov.					30:33	Antrihabitans stalactiti gen. nov.	0:33	Antrihabitans stalactiti gen. nov.	0:33	Antrihabitans stalactiti gen. nov., sp.
32910752	3	73	theme	gene	383:386	arg1	tree					388:391	The 16S rRNA gene tree	370:391	The 16S rRNA gene tree	370:391	The 16S rRNA gene tree showed that the novel isolates occupied an independent position separated from recognized genera of the family Nocardiaceae.
32910752	5	74	theme	family	769:774	arg1	Nocardiaceae					776:787	the family Nocardiaceae	765:787	the family Nocardiaceae	765:787	The 16S rRNA gene sequence simairity between the isolate and members of the family Nocardiaceae was below 96.7 %.
32910752	7	75	theme	Whole-cell	894:903	arg1	sugars					905:910	Whole-cell sugars	894:910	Whole-cell sugars	894:910	Whole-cell sugars consisted of arabinose, galactose and glucose.
32910752	1	76	theme	family	66:71	arg1	Nocardiaceae					73:84	the family Nocardiaceae	62:84	the family Nocardiaceae isolated from a cave	62:105	nov., a new member of the family Nocardiaceae isolated from a cave.
32910752	13	77	theme	Antrihabitans	1634:1646	arg1	nov.					1664:1667	the name Antrihabitans stalactiti gen. nov.	1625:1667	the name Antrihabitans stalactiti gen. nov.	1625:1667	On the basis of combination of morphological and chemotaxonomic differences, in addition to phylogenetic distinctness, the novel isolates are considered to constitute members of a novel species of a new genus in the family Nocardiaceae, for which the name Antrihabitans stalactiti gen. nov., sp.
32910752	13	78	theme	phylogenetic	1470:1481	arg1	distinctness					1483:1494	phylogenetic distinctness	1470:1494	phylogenetic distinctness	1470:1494	On the basis of combination of morphological and chemotaxonomic differences, in addition to phylogenetic distinctness, the novel isolates are considered to constitute members of a novel species of a new genus in the family Nocardiaceae, for which the name Antrihabitans stalactiti gen. nov., sp.
32910752	6	79	theme	cell-wall	811:819	arg1	acid					859:862	meso-diaminopimelic acid	839:862	meso-diaminopimelic acid	839:862	The cell-wall peptidoglycan was meso-diaminopimelic acid as a diagnostic diamino acid.
32910752	6	79	theme	cell-wall	811:819	arg1	peptidoglycan					821:833	The cell-wall peptidoglycan	807:833	The cell-wall peptidoglycan	807:833	The cell-wall peptidoglycan was meso-diaminopimelic acid as a diagnostic diamino acid.
32910752	3	80	theme	16S	374:376	arg1	tree					388:391	The 16S rRNA gene tree	370:391	The 16S rRNA gene tree	370:391	The 16S rRNA gene tree showed that the novel isolates occupied an independent position separated from recognized genera of the family Nocardiaceae.
32910752	13	81	theme	gen.	1659:1662	arg1	nov.					1664:1667	the name Antrihabitans stalactiti gen. nov.	1625:1667	the name Antrihabitans stalactiti gen. nov.	1625:1667	On the basis of combination of morphological and chemotaxonomic differences, in addition to phylogenetic distinctness, the novel isolates are considered to constitute members of a novel species of a new genus in the family Nocardiaceae, for which the name Antrihabitans stalactiti gen. nov., sp.
32910752	4	82	with	strain	618:623	arg1	identity					683:690	66.2 % average amino acid identity	657:690	66.2 % average amino acid identity	657:690	In the 92 core gene-based phylogenomic analysis, strain YC2-7T was loosely associated with the type strain of Aldersonia kummingensis with 66.2 % average amino acid identity.
32910752	3	83	used	occupied	424:431	arg2	isolates					415:422	the novel isolates	405:422	the novel isolates	405:422	The 16S rRNA gene tree showed that the novel isolates occupied an independent position separated from recognized genera of the family Nocardiaceae.
32910752	10	84	theme	cellular	1156:1163	arg1	acids					1171:1175	The cellular fatty acids	1152:1175	The cellular fatty acids	1152:1175	The cellular fatty acids consisted mainly of saturated and unsaturated components with small amounts of tuberculostearic acid.
32910752	0	85	dep	sp	36:37	arg1	nov.					30:33	Antrihabitans stalactiti gen. nov.	0:33	Antrihabitans stalactiti gen. nov.	0:33	Antrihabitans stalactiti gen. nov., sp.
32910752	11	86	theme	Mycolic	1279:1285	arg1	acids					1287:1291	Mycolic acids	1279:1291	Mycolic acids of 52-58 carbon atoms	1279:1313	Mycolic acids of 52-58 carbon atoms were present.
32910752	2	87	theme	Korea	298:302	arg1	larva					265:269	larva cave	265:274	larva cave	265:274	Two Gram-stain-positive, strictly aerobic, non-spore-forming actinobacterial strains, designated YC2-7T and YC5-17, were isolated from the Yongcheondonggul (larva cave) in Jeju, Republic of Korea and their taxonomic ranks were examined by a polyphasic approach.
32910752	2	87	theme	Korea	298:302	arg1	Yongcheondonggul					247:262	the Yongcheondonggul	243:262	the Yongcheondonggul (larva cave) in Jeju, Republic of Korea	243:302	Two Gram-stain-positive, strictly aerobic, non-spore-forming actinobacterial strains, designated YC2-7T and YC5-17, were isolated from the Yongcheondonggul (larva cave) in Jeju, Republic of Korea and their taxonomic ranks were examined by a polyphasic approach.
32910752	13	88	from	genus	1581:1585	arg1	Nocardiaceae					1601:1612	the family Nocardiaceae	1590:1612	the family Nocardiaceae	1590:1612	On the basis of combination of morphological and chemotaxonomic differences, in addition to phylogenetic distinctness, the novel isolates are considered to constitute members of a novel species of a new genus in the family Nocardiaceae, for which the name Antrihabitans stalactiti gen. nov., sp.
32910752	4	89	theme	strain	567:572	arg1	YC2-7T					574:579	strain YC2-7T	567:579	strain YC2-7T	567:579	In the 92 core gene-based phylogenomic analysis, strain YC2-7T was loosely associated with the type strain of Aldersonia kummingensis with 66.2 % average amino acid identity.
32910752	13	90	theme	morphological	1409:1421	arg1	differences					1442:1452	morphological and chemotaxonomic differences	1409:1452	morphological and chemotaxonomic differences	1409:1452	On the basis of combination of morphological and chemotaxonomic differences, in addition to phylogenetic distinctness, the novel isolates are considered to constitute members of a novel species of a new genus in the family Nocardiaceae, for which the name Antrihabitans stalactiti gen. nov., sp.
32804604	3	0	theme	Republic	277:284	arg1	town					255:258	the town	251:258	the town of Trebon, Czech Republic	251:284	A novel actinobacterial strain, designated 15TR583T, was isolated from a waterlogged acidic soil collected near the town of Trebon, Czech Republic, and was subjected to a polyphasic taxonomic characterization.
32804604	12	1	theme	peptidoglycan	1364:1376	arg1	type					1378:1381	The peptidoglycan type	1360:1381	The peptidoglycan type	1360:1381	The peptidoglycan type was A1γ meso-diaminopimelic acid.
32804604	12	1	theme	peptidoglycan	1364:1376	arg1	acid					1411:1414	A1γ meso-diaminopimelic acid	1387:1414	A1γ meso-diaminopimelic acid	1387:1414	The peptidoglycan type was A1γ meso-diaminopimelic acid.
32804604	8	2	theme	fatty	898:902	arg1	pattern					909:915	The fatty acid pattern	894:915	The fatty acid pattern	894:915	The fatty acid pattern differed from all patterns currently described for actinobacterial genera.
32804604	15	3	theme	strain	1650:1655	arg1	109105T					1680:1686	type strain 15TR583T=CCM 8942T=DSM 109105T	1645:1686	type strain 15TR583T=CCM 8942T=DSM 109105T	1645:1686	nov. (type strain 15TR583T=CCM 8942T=DSM 109105T), within Treboniaceae fam.
32804604	15	3	theme	strain	1650:1655	arg1	nov					1639:1641	nov	1639:1641	nov	1639:1641	nov. (type strain 15TR583T=CCM 8942T=DSM 109105T), within Treboniaceae fam.
32804604	4	4	theme	related	484:490	arg1	descent					476:482	descent	476:482	descent related to the order Streptosporangiales, class Actinomycetia	476:544	Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences revealed that the organism forms an individual line of descent related to the order Streptosporangiales, class Actinomycetia.
32804604	14	5	theme	type	1606:1609	arg1	sp					1635:1636	the type species Trebonia kvetii sp	1602:1636	the type species Trebonia kvetii sp	1602:1636	The distinct phylogenetic position and unusual combination of chemotaxonomic characteristics justify the proposal of Trebonia gen. nov., with the type species Trebonia kvetii sp.
32804604	3	6	theme	novel	141:145	arg1	strain					163:168	A novel actinobacterial strain	139:168	A novel actinobacterial strain	139:168	A novel actinobacterial strain, designated 15TR583T, was isolated from a waterlogged acidic soil collected near the town of Trebon, Czech Republic, and was subjected to a polyphasic taxonomic characterization.
32804604	3	7	attach	isolated	196:203	arg2	strain					163:168	A novel actinobacterial strain	139:168	A novel actinobacterial strain	139:168	A novel actinobacterial strain, designated 15TR583T, was isolated from a waterlogged acidic soil collected near the town of Trebon, Czech Republic, and was subjected to a polyphasic taxonomic characterization.
32804604	3	7	attach	isolated	196:203	arg1	soil					231:234	a waterlogged acidic soil	210:234	a waterlogged acidic soil collected near the town of Trebon, Czech Republic	210:284	A novel actinobacterial strain, designated 15TR583T, was isolated from a waterlogged acidic soil collected near the town of Trebon, Czech Republic, and was subjected to a polyphasic taxonomic characterization.
32804604	4	8	theme	descent	476:482	arg1	line					468:471	an individual line	454:471	an individual line of descent related to the order Streptosporangiales, class Actinomycetia	454:544	Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences revealed that the organism forms an individual line of descent related to the order Streptosporangiales, class Actinomycetia.
32804604	3	9	theme	actinobacterial	147:161	arg1	strain					163:168	A novel actinobacterial strain	139:168	A novel actinobacterial strain	139:168	A novel actinobacterial strain, designated 15TR583T, was isolated from a waterlogged acidic soil collected near the town of Trebon, Czech Republic, and was subjected to a polyphasic taxonomic characterization.
32804604	10	10	theme	unidentified	1253:1264	arg1	aminolipids					1266:1276	two unidentified aminolipids	1249:1276	two unidentified aminolipids	1249:1276	Polar lipids were composed of six unidentified glycolipids, an unidentified phosphoglycolipid, two unidentified phospholipids and two unidentified aminolipids.
32804604	3	11	theme	polyphasic	310:319	arg1	characterization					331:346	a polyphasic taxonomic characterization	308:346	a polyphasic taxonomic characterization	308:346	A novel actinobacterial strain, designated 15TR583T, was isolated from a waterlogged acidic soil collected near the town of Trebon, Czech Republic, and was subjected to a polyphasic taxonomic characterization.
32804604	14	12	theme	Trebonia	1577:1584	arg1	nov.					1591:1594	Trebonia gen. nov.	1577:1594	Trebonia gen. nov.	1577:1594	The distinct phylogenetic position and unusual combination of chemotaxonomic characteristics justify the proposal of Trebonia gen. nov., with the type species Trebonia kvetii sp.
32804604	14	13	theme	characteristics	1537:1551	arg1	position					1486:1493	The distinct phylogenetic position	1460:1493	The distinct phylogenetic position	1460:1493	The distinct phylogenetic position and unusual combination of chemotaxonomic characteristics justify the proposal of Trebonia gen. nov., with the type species Trebonia kvetii sp.
32804604	14	13	theme	characteristics	1537:1551	arg1	combination					1507:1517	unusual combination	1499:1517	unusual combination of chemotaxonomic characteristics	1499:1551	The distinct phylogenetic position and unusual combination of chemotaxonomic characteristics justify the proposal of Trebonia gen. nov., with the type species Trebonia kvetii sp.
32804604	4	14	theme	16S	380:382	arg1	rRNA					384:387	16S rRNA	380:387	16S rRNA gene	380:392	Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences revealed that the organism forms an individual line of descent related to the order Streptosporangiales, class Actinomycetia.
32804604	5	15	theme	gene	582:585	arg1	similarity					596:605	highest 16S rRNA gene sequence similarity	565:605	highest 16S rRNA gene sequence similarity	565:605	The strain shared highest 16S rRNA gene sequence similarity, yet of only 92.8%, with Actinocorallia aurea IFO 14752T.
32804604	10	16	theme	unidentified	1153:1164	arg1	glycolipids					1166:1176	six unidentified glycolipids	1149:1176	six unidentified glycolipids	1149:1176	Polar lipids were composed of six unidentified glycolipids, an unidentified phosphoglycolipid, two unidentified phospholipids and two unidentified aminolipids.
32804604	14	17	theme	nov.	1591:1594	arg1	proposal					1565:1572	the proposal	1561:1572	the proposal of Trebonia gen. nov.	1561:1594	The distinct phylogenetic position and unusual combination of chemotaxonomic characteristics justify the proposal of Trebonia gen. nov., with the type species Trebonia kvetii sp.
32804604	3	18	theme	acidic	224:229	arg1	soil					231:234	a waterlogged acidic soil	210:234	a waterlogged acidic soil collected near the town of Trebon, Czech Republic	210:284	A novel actinobacterial strain, designated 15TR583T, was isolated from a waterlogged acidic soil collected near the town of Trebon, Czech Republic, and was subjected to a polyphasic taxonomic characterization.
32804604	5	19	theme	sequence	587:594	arg1	similarity					596:605	highest 16S rRNA gene sequence similarity	565:605	highest 16S rRNA gene sequence similarity	565:605	The strain shared highest 16S rRNA gene sequence similarity, yet of only 92.8%, with Actinocorallia aurea IFO 14752T.
32804604	1	20	theme	acidophilic	40:50	arg1	actinobacterium					52:66	an acidophilic actinobacterium	37:66	an acidophilic actinobacterium	37:66	nov., an acidophilic actinobacterium, and proposal of the new actinobacterial family Treboniaceae fam.
32804604	13	21	theme	genomic	1421:1427	arg1	%					1457:1457	69.7 mol%	1449:1457	69.7 mol%	1449:1457	The genomic DNA G+C content was 69.7 mol%.
32804604	13	21	theme	genomic	1421:1427	arg1	content					1437:1443	The genomic DNA G+C content	1417:1443	The genomic DNA G+C content	1417:1443	The genomic DNA G+C content was 69.7 mol%.
32804604	4	22	theme	individual	457:466	arg1	line					468:471	an individual line	454:471	an individual line of descent related to the order Streptosporangiales, class Actinomycetia	454:544	Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences revealed that the organism forms an individual line of descent related to the order Streptosporangiales, class Actinomycetia.
32804604	6	23	theme	mycelium	754:761	arg1	colonies					690:697	white colonies	684:697	white colonies of aerobic, Gram-stain-positive, unbranching substrate mycelium bearing single spores at hyphae tips	684:798	The strain grew in white colonies of aerobic, Gram-stain-positive, unbranching substrate mycelium bearing single spores at hyphae tips.
32804604	14	24	theme	species	1611:1617	arg1	sp					1635:1636	the type species Trebonia kvetii sp	1602:1636	the type species Trebonia kvetii sp	1602:1636	The distinct phylogenetic position and unusual combination of chemotaxonomic characteristics justify the proposal of Trebonia gen. nov., with the type species Trebonia kvetii sp.
32804604	7	25	theme	major	805:809	arg1	0					845:845	0	845:845	0	845:845	The major fatty acids (>10%) were iso-C16 : 0, C16 : 0, iso-C17 : 1ω9 and 10-methyl-C17 : 0.
32804604	7	25	theme	major	805:809	arg1	acids					817:821	The major fatty acids	801:821	The major fatty acids (>10%)	801:828	The major fatty acids (>10%) were iso-C16 : 0, C16 : 0, iso-C17 : 1ω9 and 10-methyl-C17 : 0.
32804604	7	25	theme	major	805:809	arg1	%					827:827	>10%	824:827	>10%	824:827	The major fatty acids (>10%) were iso-C16 : 0, C16 : 0, iso-C17 : 1ω9 and 10-methyl-C17 : 0.
32804604	13	26	theme	DNA	1429:1431	arg1	%					1457:1457	69.7 mol%	1449:1457	69.7 mol%	1449:1457	The genomic DNA G+C content was 69.7 mol%.
32804604	13	26	theme	DNA	1429:1431	arg1	content					1437:1443	The genomic DNA G+C content	1417:1443	The genomic DNA G+C content	1417:1443	The genomic DNA G+C content was 69.7 mol%.
32804604	0	27	theme	kvetii	9:14	arg1	nov.					21:24	Trebonia kvetii gen. nov.	0:24	Trebonia kvetii gen. nov.	0:24	Trebonia kvetii gen. nov., sp.
32804604	10	28	theme	unidentified	1182:1193	arg1	phosphoglycolipid					1195:1211	an unidentified phosphoglycolipid	1179:1211	an unidentified phosphoglycolipid	1179:1211	Polar lipids were composed of six unidentified glycolipids, an unidentified phosphoglycolipid, two unidentified phospholipids and two unidentified aminolipids.
32804604	8	29	theme	actinobacterial	968:982	arg1	genera					984:989	actinobacterial genera	968:989	actinobacterial genera	968:989	The fatty acid pattern differed from all patterns currently described for actinobacterial genera.
32804604	6	30	theme	substrate	744:752	arg1	mycelium					754:761	aerobic, Gram-stain-positive, unbranching substrate mycelium	702:761	aerobic, Gram-stain-positive, unbranching substrate mycelium bearing single spores at hyphae tips	702:798	The strain grew in white colonies of aerobic, Gram-stain-positive, unbranching substrate mycelium bearing single spores at hyphae tips.
32804604	11	31	contain	contained	1297:1305	arg1	sugars					1290:1295	Whole-cell sugars	1279:1295	Whole-cell sugars	1279:1295	Whole-cell sugars contained galactose, xylose and arabinose as major components.
32804604	11	31	contain	contained	1297:1305	arg2	arabinose					1329:1337	arabinose	1329:1337	arabinose	1329:1337	Whole-cell sugars contained galactose, xylose and arabinose as major components.
32804604	11	31	contain	contained	1297:1305	arg2	xylose					1318:1323	xylose	1318:1323	xylose	1318:1323	Whole-cell sugars contained galactose, xylose and arabinose as major components.
32804604	11	31	contain	contained	1297:1305	arg2	components					1348:1357	major components	1342:1357	major components	1342:1357	Whole-cell sugars contained galactose, xylose and arabinose as major components.
32804604	11	31	contain	contained	1297:1305	arg2	galactose					1307:1315	galactose	1307:1315	galactose	1307:1315	Whole-cell sugars contained galactose, xylose and arabinose as major components.
32804604	7	32	theme	fatty	811:815	arg1	0					845:845	0	845:845	0	845:845	The major fatty acids (>10%) were iso-C16 : 0, C16 : 0, iso-C17 : 1ω9 and 10-methyl-C17 : 0.
32804604	7	32	theme	fatty	811:815	arg1	acids					817:821	The major fatty acids	801:821	The major fatty acids (>10%)	801:828	The major fatty acids (>10%) were iso-C16 : 0, C16 : 0, iso-C17 : 1ω9 and 10-methyl-C17 : 0.
32804604	7	32	theme	fatty	811:815	arg1	%					827:827	>10%	824:827	>10%	824:827	The major fatty acids (>10%) were iso-C16 : 0, C16 : 0, iso-C17 : 1ω9 and 10-methyl-C17 : 0.
32804604	13	33	theme	G+C	1433:1435	arg1	%					1457:1457	69.7 mol%	1449:1457	69.7 mol%	1449:1457	The genomic DNA G+C content was 69.7 mol%.
32804604	13	33	theme	G+C	1433:1435	arg1	content					1437:1443	The genomic DNA G+C content	1417:1443	The genomic DNA G+C content	1417:1443	The genomic DNA G+C content was 69.7 mol%.
32804604	0	34	theme	Trebonia	0:7	arg1	nov.					21:24	Trebonia kvetii gen. nov.	0:24	Trebonia kvetii gen. nov.	0:24	Trebonia kvetii gen. nov., sp.
32804604	4	35	theme	class	526:530	arg1	Streptosporangiales					505:523	the order Streptosporangiales	495:523	the order Streptosporangiales	495:523	Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences revealed that the organism forms an individual line of descent related to the order Streptosporangiales, class Actinomycetia.
32804604	4	35	theme	class	526:530	arg1	Actinomycetia					532:544	class Actinomycetia	526:544	class Actinomycetia	526:544	Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences revealed that the organism forms an individual line of descent related to the order Streptosporangiales, class Actinomycetia.
32804604	3	36	theme	waterlogged	212:222	arg1	soil					231:234	a waterlogged acidic soil	210:234	a waterlogged acidic soil collected near the town of Trebon, Czech Republic	210:284	A novel actinobacterial strain, designated 15TR583T, was isolated from a waterlogged acidic soil collected near the town of Trebon, Czech Republic, and was subjected to a polyphasic taxonomic characterization.
32804604	4	37	theme	Phylogenetic	349:360	arg1	analysis					362:369	Phylogenetic analysis	349:369	Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences	349:419	Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences revealed that the organism forms an individual line of descent related to the order Streptosporangiales, class Actinomycetia.
32804604	15	38	theme	Treboniaceae	1697:1708	arg1	fam					1710:1712	Treboniaceae fam	1697:1712	Treboniaceae fam	1697:1712	nov. (type strain 15TR583T=CCM 8942T=DSM 109105T), within Treboniaceae fam.
32804604	14	39	theme	unusual	1499:1505	arg1	combination					1507:1517	unusual combination	1499:1517	unusual combination of chemotaxonomic characteristics	1499:1551	The distinct phylogenetic position and unusual combination of chemotaxonomic characteristics justify the proposal of Trebonia gen. nov., with the type species Trebonia kvetii sp.
32804604	3	40	theme	taxonomic	321:329	arg1	characterization					331:346	a polyphasic taxonomic characterization	308:346	a polyphasic taxonomic characterization	308:346	A novel actinobacterial strain, designated 15TR583T, was isolated from a waterlogged acidic soil collected near the town of Trebon, Czech Republic, and was subjected to a polyphasic taxonomic characterization.
32804604	9	41	theme	other	1087:1091	arg1	families					1109:1116	other actinobacterial families	1087:1116	other actinobacterial families	1087:1116	The organism contained as major menaquinones MK9(H6) and MK9(H8), which differentiated it from other actinobacterial families.
32804604	5	42	theme	highest	565:571	arg1	similarity					596:605	highest 16S rRNA gene sequence similarity	565:605	highest 16S rRNA gene sequence similarity	565:605	The strain shared highest 16S rRNA gene sequence similarity, yet of only 92.8%, with Actinocorallia aurea IFO 14752T.
32804604	5	43	theme	16S	573:575	arg1	similarity					596:605	highest 16S rRNA gene sequence similarity	565:605	highest 16S rRNA gene sequence similarity	565:605	The strain shared highest 16S rRNA gene sequence similarity, yet of only 92.8%, with Actinocorallia aurea IFO 14752T.
32804604	15	44	dep	nov	1639:1641	arg1	fam					1710:1712	Treboniaceae fam	1697:1712	Treboniaceae fam	1697:1712	nov. (type strain 15TR583T=CCM 8942T=DSM 109105T), within Treboniaceae fam.
32804604	0	45	theme	gen.	16:19	arg1	nov.					21:24	Trebonia kvetii gen. nov.	0:24	Trebonia kvetii gen. nov.	0:24	Trebonia kvetii gen. nov., sp.
32804604	14	46	theme	chemotaxonomic	1522:1535	arg1	characteristics					1537:1551	chemotaxonomic characteristics	1522:1551	chemotaxonomic characteristics	1522:1551	The distinct phylogenetic position and unusual combination of chemotaxonomic characteristics justify the proposal of Trebonia gen. nov., with the type species Trebonia kvetii sp.
32804604	1	47	dep	fam	129:131	arg1	Treboniaceae					116:127	Treboniaceae	116:127	Treboniaceae	116:127	nov., an acidophilic actinobacterium, and proposal of the new actinobacterial family Treboniaceae fam.
32804604	9	48	theme	major	1018:1022	arg1	H6					1041:1042	H6	1041:1042	H6	1041:1042	The organism contained as major menaquinones MK9(H6) and MK9(H8), which differentiated it from other actinobacterial families.
32804604	9	48	theme	major	1018:1022	arg1	MK9					1037:1039	major menaquinones MK9	1018:1039	major menaquinones MK9(H6)	1018:1043	The organism contained as major menaquinones MK9(H6) and MK9(H8), which differentiated it from other actinobacterial families.
32804604	7	49	dep	C16	848:850	arg1	0					891:891	0	891:891	0	891:891	The major fatty acids (>10%) were iso-C16 : 0, C16 : 0, iso-C17 : 1ω9 and 10-methyl-C17 : 0.
32804604	7	49	dep	C16	848:850	arg1	10-methyl-C17					875:887	10-methyl-C17	875:887	10-methyl-C17	875:887	The major fatty acids (>10%) were iso-C16 : 0, C16 : 0, iso-C17 : 1ω9 and 10-methyl-C17 : 0.
32804604	7	49	dep	C16	848:850	arg1	1ω9					867:869	1ω9	867:869	1ω9	867:869	The major fatty acids (>10%) were iso-C16 : 0, C16 : 0, iso-C17 : 1ω9 and 10-methyl-C17 : 0.
32804604	9	50	theme	menaquinones	1024:1035	arg1	H6					1041:1042	H6	1041:1042	H6	1041:1042	The organism contained as major menaquinones MK9(H6) and MK9(H8), which differentiated it from other actinobacterial families.
32804604	9	50	theme	menaquinones	1024:1035	arg1	MK9					1037:1039	major menaquinones MK9	1018:1039	major menaquinones MK9(H6)	1018:1043	The organism contained as major menaquinones MK9(H6) and MK9(H8), which differentiated it from other actinobacterial families.
32804604	11	51	theme	Whole-cell	1279:1288	arg1	sugars					1290:1295	Whole-cell sugars	1279:1295	Whole-cell sugars	1279:1295	Whole-cell sugars contained galactose, xylose and arabinose as major components.
32804604	12	52	theme	A1γ	1387:1389	arg1	type					1378:1381	The peptidoglycan type	1360:1381	The peptidoglycan type	1360:1381	The peptidoglycan type was A1γ meso-diaminopimelic acid.
32804604	12	52	theme	A1γ	1387:1389	arg1	acid					1411:1414	A1γ meso-diaminopimelic acid	1387:1414	A1γ meso-diaminopimelic acid	1387:1414	The peptidoglycan type was A1γ meso-diaminopimelic acid.
32804604	15	53	theme	type	1645:1648	arg1	109105T					1680:1686	type strain 15TR583T=CCM 8942T=DSM 109105T	1645:1686	type strain 15TR583T=CCM 8942T=DSM 109105T	1645:1686	nov. (type strain 15TR583T=CCM 8942T=DSM 109105T), within Treboniaceae fam.
32804604	15	53	theme	type	1645:1648	arg1	nov					1639:1641	nov	1639:1641	nov	1639:1641	nov. (type strain 15TR583T=CCM 8942T=DSM 109105T), within Treboniaceae fam.
32804604	8	54	theme	acid	904:907	arg1	pattern					909:915	The fatty acid pattern	894:915	The fatty acid pattern	894:915	The fatty acid pattern differed from all patterns currently described for actinobacterial genera.
32804604	6	55	theme	single	771:776	arg1	spores					778:783	single spores	771:783	single spores	771:783	The strain grew in white colonies of aerobic, Gram-stain-positive, unbranching substrate mycelium bearing single spores at hyphae tips.
32804604	14	56	theme	kvetii	1628:1633	arg1	sp					1635:1636	the type species Trebonia kvetii sp	1602:1636	the type species Trebonia kvetii sp	1602:1636	The distinct phylogenetic position and unusual combination of chemotaxonomic characteristics justify the proposal of Trebonia gen. nov., with the type species Trebonia kvetii sp.
32804604	0	57	dep	sp	27:28	arg1	nov.					21:24	Trebonia kvetii gen. nov.	0:24	Trebonia kvetii gen. nov.	0:24	Trebonia kvetii gen. nov., sp.
32804604	9	58	theme	actinobacterial	1093:1107	arg1	families					1109:1116	other actinobacterial families	1087:1116	other actinobacterial families	1087:1116	The organism contained as major menaquinones MK9(H6) and MK9(H8), which differentiated it from other actinobacterial families.
32804604	14	59	theme	gen.	1586:1589	arg1	nov.					1591:1594	Trebonia gen. nov.	1577:1594	Trebonia gen. nov.	1577:1594	The distinct phylogenetic position and unusual combination of chemotaxonomic characteristics justify the proposal of Trebonia gen. nov., with the type species Trebonia kvetii sp.
32804604	11	60	theme	major	1342:1346	arg1	galactose					1307:1315	galactose	1307:1315	galactose	1307:1315	Whole-cell sugars contained galactose, xylose and arabinose as major components.
32804604	11	60	theme	major	1342:1346	arg1	arabinose					1329:1337	arabinose	1329:1337	arabinose	1329:1337	Whole-cell sugars contained galactose, xylose and arabinose as major components.
32804604	11	60	theme	major	1342:1346	arg1	components					1348:1357	major components	1342:1357	major components	1342:1357	Whole-cell sugars contained galactose, xylose and arabinose as major components.
32804604	11	60	theme	major	1342:1346	arg1	xylose					1318:1323	xylose	1318:1323	xylose	1318:1323	Whole-cell sugars contained galactose, xylose and arabinose as major components.
32804604	5	61	theme	Actinocorallia	632:645	arg1	14752T					657:662	Actinocorallia aurea IFO 14752T	632:662	Actinocorallia aurea IFO 14752T	632:662	The strain shared highest 16S rRNA gene sequence similarity, yet of only 92.8%, with Actinocorallia aurea IFO 14752T.
32804604	14	62	theme	Trebonia	1619:1626	arg1	sp					1635:1636	the type species Trebonia kvetii sp	1602:1636	the type species Trebonia kvetii sp	1602:1636	The distinct phylogenetic position and unusual combination of chemotaxonomic characteristics justify the proposal of Trebonia gen. nov., with the type species Trebonia kvetii sp.
32804604	12	63	theme	meso-diaminopimelic	1391:1409	arg1	type					1378:1381	The peptidoglycan type	1360:1381	The peptidoglycan type	1360:1381	The peptidoglycan type was A1γ meso-diaminopimelic acid.
32804604	12	63	theme	meso-diaminopimelic	1391:1409	arg1	acid					1411:1414	A1γ meso-diaminopimelic acid	1387:1414	A1γ meso-diaminopimelic acid	1387:1414	The peptidoglycan type was A1γ meso-diaminopimelic acid.
32804604	6	64	theme	aerobic	702:708	arg1	mycelium					754:761	aerobic, Gram-stain-positive, unbranching substrate mycelium	702:761	aerobic, Gram-stain-positive, unbranching substrate mycelium bearing single spores at hyphae tips	702:798	The strain grew in white colonies of aerobic, Gram-stain-positive, unbranching substrate mycelium bearing single spores at hyphae tips.
32804604	1	65	theme	new	89:91	arg1	family					109:114	the new actinobacterial family	85:114	the new actinobacterial family	85:114	nov., an acidophilic actinobacterium, and proposal of the new actinobacterial family Treboniaceae fam.
32804604	4	66	theme	order	499:503	arg1	Streptosporangiales					505:523	the order Streptosporangiales	495:523	the order Streptosporangiales	495:523	Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences revealed that the organism forms an individual line of descent related to the order Streptosporangiales, class Actinomycetia.
32804604	4	66	theme	order	499:503	arg1	Actinomycetia					532:544	class Actinomycetia	526:544	class Actinomycetia	526:544	Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences revealed that the organism forms an individual line of descent related to the order Streptosporangiales, class Actinomycetia.
32804604	5	67	theme	aurea	647:651	arg1	14752T					657:662	Actinocorallia aurea IFO 14752T	632:662	Actinocorallia aurea IFO 14752T	632:662	The strain shared highest 16S rRNA gene sequence similarity, yet of only 92.8%, with Actinocorallia aurea IFO 14752T.
32804604	4	68	theme	rRNA	384:387	arg1	gene					389:392	16S rRNA gene	380:392	16S rRNA gene	380:392	Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences revealed that the organism forms an individual line of descent related to the order Streptosporangiales, class Actinomycetia.
32804604	5	69	theme	rRNA	577:580	arg1	similarity					596:605	highest 16S rRNA gene sequence similarity	565:605	highest 16S rRNA gene sequence similarity	565:605	The strain shared highest 16S rRNA gene sequence similarity, yet of only 92.8%, with Actinocorallia aurea IFO 14752T.
32804604	1	70	theme	actinobacterial	93:107	arg1	family					109:114	the new actinobacterial family	85:114	the new actinobacterial family	85:114	nov., an acidophilic actinobacterium, and proposal of the new actinobacterial family Treboniaceae fam.
32804604	10	71	theme	Polar	1119:1123	arg1	lipids					1125:1130	Polar lipids	1119:1130	Polar lipids	1119:1130	Polar lipids were composed of six unidentified glycolipids, an unidentified phosphoglycolipid, two unidentified phospholipids and two unidentified aminolipids.
32804604	5	72	theme	IFO	653:655	arg1	14752T					657:662	Actinocorallia aurea IFO 14752T	632:662	Actinocorallia aurea IFO 14752T	632:662	The strain shared highest 16S rRNA gene sequence similarity, yet of only 92.8%, with Actinocorallia aurea IFO 14752T.
32804604	4	73	theme	whole-genome	398:409	arg1	sequences					411:419	whole-genome sequences	398:419	whole-genome sequences	398:419	Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences revealed that the organism forms an individual line of descent related to the order Streptosporangiales, class Actinomycetia.
32804604	14	74	theme	distinct	1464:1471	arg1	position					1486:1493	The distinct phylogenetic position	1460:1493	The distinct phylogenetic position	1460:1493	The distinct phylogenetic position and unusual combination of chemotaxonomic characteristics justify the proposal of Trebonia gen. nov., with the type species Trebonia kvetii sp.
32804604	1	75	theme	family	109:114	arg1	actinobacterium					52:66	an acidophilic actinobacterium	37:66	an acidophilic actinobacterium	37:66	nov., an acidophilic actinobacterium, and proposal of the new actinobacterial family Treboniaceae fam.
32804604	1	75	theme	family	109:114	arg1	proposal					73:80	proposal	73:80	proposal of the new actinobacterial family	73:114	nov., an acidophilic actinobacterium, and proposal of the new actinobacterial family Treboniaceae fam.
32804604	1	75	theme	family	109:114	arg1	nov.					31:34	nov.	31:34	nov.	31:34	nov., an acidophilic actinobacterium, and proposal of the new actinobacterial family Treboniaceae fam.
32804604	15	76	theme	15TR583T=CCM	1657:1668	arg1	109105T					1680:1686	type strain 15TR583T=CCM 8942T=DSM 109105T	1645:1686	type strain 15TR583T=CCM 8942T=DSM 109105T	1645:1686	nov. (type strain 15TR583T=CCM 8942T=DSM 109105T), within Treboniaceae fam.
32804604	15	76	theme	15TR583T=CCM	1657:1668	arg1	nov					1639:1641	nov	1639:1641	nov	1639:1641	nov. (type strain 15TR583T=CCM 8942T=DSM 109105T), within Treboniaceae fam.
32804604	10	77	theme	unidentified	1218:1229	arg1	phospholipids					1231:1243	two unidentified phospholipids	1214:1243	two unidentified phospholipids	1214:1243	Polar lipids were composed of six unidentified glycolipids, an unidentified phosphoglycolipid, two unidentified phospholipids and two unidentified aminolipids.
32804604	6	78	theme	hyphae	788:793	arg1	tips					795:798	hyphae tips	788:798	hyphae tips	788:798	The strain grew in white colonies of aerobic, Gram-stain-positive, unbranching substrate mycelium bearing single spores at hyphae tips.
32804604	13	79	theme	mol	1454:1456	arg1	%					1457:1457	69.7 mol%	1449:1457	69.7 mol%	1449:1457	The genomic DNA G+C content was 69.7 mol%.
32804604	13	79	theme	mol	1454:1456	arg1	content					1437:1443	The genomic DNA G+C content	1417:1443	The genomic DNA G+C content	1417:1443	The genomic DNA G+C content was 69.7 mol%.
32804604	6	80	theme	white	684:688	arg1	colonies					690:697	white colonies	684:697	white colonies of aerobic, Gram-stain-positive, unbranching substrate mycelium bearing single spores at hyphae tips	684:798	The strain grew in white colonies of aerobic, Gram-stain-positive, unbranching substrate mycelium bearing single spores at hyphae tips.
32804604	15	81	theme	8942T=DSM	1670:1678	arg1	109105T					1680:1686	type strain 15TR583T=CCM 8942T=DSM 109105T	1645:1686	type strain 15TR583T=CCM 8942T=DSM 109105T	1645:1686	nov. (type strain 15TR583T=CCM 8942T=DSM 109105T), within Treboniaceae fam.
32804604	15	81	theme	8942T=DSM	1670:1678	arg1	nov					1639:1641	nov	1639:1641	nov	1639:1641	nov. (type strain 15TR583T=CCM 8942T=DSM 109105T), within Treboniaceae fam.
32804604	14	82	theme	phylogenetic	1473:1484	arg1	position					1486:1493	The distinct phylogenetic position	1460:1493	The distinct phylogenetic position	1460:1493	The distinct phylogenetic position and unusual combination of chemotaxonomic characteristics justify the proposal of Trebonia gen. nov., with the type species Trebonia kvetii sp.
32804604	6	83	dep	aerobic	702:708	arg1	unbranching					732:742	unbranching	732:742	unbranching	732:742	The strain grew in white colonies of aerobic, Gram-stain-positive, unbranching substrate mycelium bearing single spores at hyphae tips.
32804604	6	83	dep	aerobic	702:708	arg1	Gram-stain-positive					711:729	Gram-stain-positive	711:729	Gram-stain-positive	711:729	The strain grew in white colonies of aerobic, Gram-stain-positive, unbranching substrate mycelium bearing single spores at hyphae tips.
32367312	7	0	theme	15068	1128:1132	arg1	pre-treatment					1134:1146	B. bifidum CGMCC 15068 pre-treatment	1111:1146	B. bifidum CGMCC 15068 pre-treatment	1111:1146	In addition, B. bifidum CGMCC 15068 pre-treatment increased the relative abundance of Akkermansia, Desulfovibrionaceae, Romboutsia, Turicibacter, Verrucomicrobiaceae, Ruminococcaceae_UCG_013, Lachnospiraceae_UCG_004, and Lactobacillus.
32367312	0	1	theme	colon	173:177	arg1	CAC					187:189	CAC	187:189	CAC	187:189	Administration of Bifidobacterium bifidum CGMCC 15068 modulates gut microbiota and metabolome in azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated colon cancer (CAC) in mice.
32367312	0	1	theme	colon	173:177	arg1	cancer					179:184	azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated colon cancer (CAC)	97:190	azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated colon cancer (CAC)	97:190	Administration of Bifidobacterium bifidum CGMCC 15068 modulates gut microbiota and metabolome in azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated colon cancer (CAC) in mice.
32367312	10	2	theme	bifidum	1677:1683	arg1	15068					1691:1695	B. bifidum CGMCC 15068	1674:1695	B. bifidum CGMCC 15068	1674:1695	These findings supported the beneficial role of B. bifidum CGMCC 15068 in intestinal health by modulating dysbiosis and the gut metabolic profile.
32367312	1	3	theme	important	229:237	arg1	role					239:242	an important role	226:242	an important role	226:242	The gut microbiota plays an important role in colorectal cancer (CRC), and the use of probiotics might be a promising intervention method.
32367312	6	4	theme	mouse	1085:1089	arg1	model					1091:1095	the CAC mouse model	1077:1095	the CAC mouse model	1077:1095	The administration of B. bifidum CGMCC 15068 attenuated tumourigenesis in the CAC mouse model.
32367312	12	5	theme	strategy	1994:2001	arg1	applications					1973:1984	the potential clinical applications	1950:1984	the potential clinical applications of this strategy in the future	1950:2015	Long-term and large-scale clinical trials are warranted for the potential clinical applications of this strategy in the future.
32367312	4	6	theme	B.	739:740	arg1	3 × 109 CFU/mL					763:776	3 × 109 CFU/mL	763:776	3 × 109 CFU/mL	763:776	B. bifidum CGMCC 15068 (3 × 109 CFU/mL) was gavaged once daily during the recovery period.
32367312	4	6	theme	B.	739:740	arg1	15068					756:760	B. bifidum CGMCC 15068	739:760	B. bifidum CGMCC 15068 (3 × 109 CFU/mL)	739:777	B. bifidum CGMCC 15068 (3 × 109 CFU/mL) was gavaged once daily during the recovery period.
32367312	10	7	theme	intestinal	1700:1709	arg1	health					1711:1716	intestinal health	1700:1716	intestinal health	1700:1716	These findings supported the beneficial role of B. bifidum CGMCC 15068 in intestinal health by modulating dysbiosis and the gut metabolic profile.
32367312	12	8	from	applications	1973:1984	arg1	future					2010:2015	future	2010:2015	future	2010:2015	Long-term and large-scale clinical trials are warranted for the potential clinical applications of this strategy in the future.
32367312	4	9	theme	CGMCC	750:754	arg1	3 × 109 CFU/mL					763:776	3 × 109 CFU/mL	763:776	3 × 109 CFU/mL	763:776	B. bifidum CGMCC 15068 (3 × 109 CFU/mL) was gavaged once daily during the recovery period.
32367312	4	9	theme	CGMCC	750:754	arg1	15068					756:760	B. bifidum CGMCC 15068	739:760	B. bifidum CGMCC 15068 (3 × 109 CFU/mL)	739:777	B. bifidum CGMCC 15068 (3 × 109 CFU/mL) was gavaged once daily during the recovery period.
32367312	3	10	theme	DSS	642:644	arg1	injection					585:593	an intra-peritoneal injection	565:593	an intra-peritoneal injection of AOM (10 mg/kg)	565:611	CAC was induced by an intra-peritoneal injection of AOM (10 mg/kg) and three 7-day cycles of 2% DSS in drinking water with a 14-day recovery period between two consecutive DSS administrations.
32367312	3	10	theme	DSS	642:644	arg1	cycles					629:634	three 7-day cycles	617:634	three 7-day cycles of 2% DSS in drinking water with a 14-day recovery period between two consecutive DSS administrations	617:736	CAC was induced by an intra-peritoneal injection of AOM (10 mg/kg) and three 7-day cycles of 2% DSS in drinking water with a 14-day recovery period between two consecutive DSS administrations.
32367312	6	11	theme	15068	1042:1046	arg1	administration					1007:1020	The administration	1003:1020	The administration of B. bifidum CGMCC 15068	1003:1046	The administration of B. bifidum CGMCC 15068 attenuated tumourigenesis in the CAC mouse model.
32367312	0	12	from	metabolome	83:92	arg1	CAC					187:189	CAC	187:189	CAC	187:189	Administration of Bifidobacterium bifidum CGMCC 15068 modulates gut microbiota and metabolome in azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated colon cancer (CAC) in mice.
32367312	0	12	from	metabolome	83:92	arg1	mice					195:198	mice	195:198	mice	195:198	Administration of Bifidobacterium bifidum CGMCC 15068 modulates gut microbiota and metabolome in azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated colon cancer (CAC) in mice.
32367312	0	12	from	metabolome	83:92	arg1	cancer					179:184	azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated colon cancer (CAC)	97:190	azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated colon cancer (CAC)	97:190	Administration of Bifidobacterium bifidum CGMCC 15068 modulates gut microbiota and metabolome in azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated colon cancer (CAC) in mice.
32367312	7	13	theme	Lactobacillus	1319:1331	arg1	abundance					1171:1179	the relative abundance	1158:1179	the relative abundance of Akkermansia, Desulfovibrionaceae, Romboutsia, Turicibacter, Verrucomicrobiaceae, Ruminococcaceae_UCG_013, Lachnospiraceae_UCG_004, and Lactobacillus	1158:1331	In addition, B. bifidum CGMCC 15068 pre-treatment increased the relative abundance of Akkermansia, Desulfovibrionaceae, Romboutsia, Turicibacter, Verrucomicrobiaceae, Ruminococcaceae_UCG_013, Lachnospiraceae_UCG_004, and Lactobacillus.
32367312	11	14	theme	promising	1851:1859	arg1	strategy					1872:1879	a promising prevention strategy	1849:1879	a promising prevention strategy for CRC	1849:1887	The manipulation of the gut microbial composition using probiotics might be a promising prevention strategy for CRC.
32367312	11	14	theme	promising	1851:1859	arg1	manipulation					1777:1788	The manipulation	1773:1788	The manipulation of the gut microbial composition using probiotics	1773:1838	The manipulation of the gut microbial composition using probiotics might be a promising prevention strategy for CRC.
32367312	0	15	theme	bifidum	34:40	arg1	CGMCC					42:46	Bifidobacterium bifidum CGMCC 15068	18:52	Bifidobacterium bifidum CGMCC 15068	18:52	Administration of Bifidobacterium bifidum CGMCC 15068 modulates gut microbiota and metabolome in azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated colon cancer (CAC) in mice.
32367312	2	16	theme	colitis-associated	504:521	arg1	model					539:543	an azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated CRC (CAC) mouse model	444:543	an azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated CRC (CAC) mouse model	444:543	The aim of our study was to investigate the beneficial effect of Bifidobacterium bifidum CGMCC 15068 on an azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated CRC (CAC) mouse model.
32367312	0	17	from	microbiota	68:77	arg1	CAC					187:189	CAC	187:189	CAC	187:189	Administration of Bifidobacterium bifidum CGMCC 15068 modulates gut microbiota and metabolome in azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated colon cancer (CAC) in mice.
32367312	0	17	from	microbiota	68:77	arg1	mice					195:198	mice	195:198	mice	195:198	Administration of Bifidobacterium bifidum CGMCC 15068 modulates gut microbiota and metabolome in azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated colon cancer (CAC) in mice.
32367312	0	17	from	microbiota	68:77	arg1	cancer					179:184	azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated colon cancer (CAC)	97:190	azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated colon cancer (CAC)	97:190	Administration of Bifidobacterium bifidum CGMCC 15068 modulates gut microbiota and metabolome in azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated colon cancer (CAC) in mice.
32367312	10	18	theme	metabolic	1754:1762	arg1	profile					1764:1770	the gut metabolic profile	1746:1770	the gut metabolic profile	1746:1770	These findings supported the beneficial role of B. bifidum CGMCC 15068 in intestinal health by modulating dysbiosis and the gut metabolic profile.
32367312	0	19	theme	-induced	145:152	arg1	CAC					187:189	CAC	187:189	CAC	187:189	Administration of Bifidobacterium bifidum CGMCC 15068 modulates gut microbiota and metabolome in azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated colon cancer (CAC) in mice.
32367312	0	19	theme	-induced	145:152	arg1	cancer					179:184	azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated colon cancer (CAC)	97:190	azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated colon cancer (CAC)	97:190	Administration of Bifidobacterium bifidum CGMCC 15068 modulates gut microbiota and metabolome in azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated colon cancer (CAC) in mice.
32367312	5	20	theme	gas	943:945	arg1	GC-MS					981:985	GC-MS	981:985	GC-MS	981:985	Then, the faecal microbial composition and metabolome were profiled using the 16S rRNA sequencing technology and gas chromatography-mass spectrometry (GC-MS), respectively.
32367312	5	20	theme	gas	943:945	arg1	spectrometry					967:978	gas chromatography-mass spectrometry	943:978	gas chromatography-mass spectrometry (GC-MS)	943:986	Then, the faecal microbial composition and metabolome were profiled using the 16S rRNA sequencing technology and gas chromatography-mass spectrometry (GC-MS), respectively.
32367312	8	21	theme	TCA	1419:1421	arg1	cycle					1412:1416	the citrate cycle	1400:1416	the citrate cycle (TCA cycle)	1400:1428	Meanwhile, B. bifidum CGMCC 15068 altered metabolites involved in the citrate cycle (TCA cycle), glycolysis, butyrate metabolism, fatty acid biosynthesis, and galactose metabolism.
32367312	8	21	theme	TCA	1419:1421	arg1	cycle					1423:1427	TCA cycle	1419:1427	TCA cycle	1419:1427	Meanwhile, B. bifidum CGMCC 15068 altered metabolites involved in the citrate cycle (TCA cycle), glycolysis, butyrate metabolism, fatty acid biosynthesis, and galactose metabolism.
32367312	3	22	theme	14-day	671:676	arg1	period					687:692	a 14-day recovery period	669:692	a 14-day recovery period between two consecutive DSS administrations	669:736	CAC was induced by an intra-peritoneal injection of AOM (10 mg/kg) and three 7-day cycles of 2% DSS in drinking water with a 14-day recovery period between two consecutive DSS administrations.
32367312	7	23	theme	CGMCC	1122:1126	arg1	pre-treatment					1134:1146	B. bifidum CGMCC 15068 pre-treatment	1111:1146	B. bifidum CGMCC 15068 pre-treatment	1111:1146	In addition, B. bifidum CGMCC 15068 pre-treatment increased the relative abundance of Akkermansia, Desulfovibrionaceae, Romboutsia, Turicibacter, Verrucomicrobiaceae, Ruminococcaceae_UCG_013, Lachnospiraceae_UCG_004, and Lactobacillus.
32367312	2	24	theme	15068	435:439	arg1	effect					395:400	the beneficial effect	380:400	the beneficial effect of Bifidobacterium bifidum CGMCC 15068 on an azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated CRC (CAC) mouse model	380:543	The aim of our study was to investigate the beneficial effect of Bifidobacterium bifidum CGMCC 15068 on an azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated CRC (CAC) mouse model.
32367312	3	25	theme	DSS	718:720	arg1	administrations					722:736	two consecutive DSS administrations	702:736	two consecutive DSS administrations	702:736	CAC was induced by an intra-peritoneal injection of AOM (10 mg/kg) and three 7-day cycles of 2% DSS in drinking water with a 14-day recovery period between two consecutive DSS administrations.
32367312	7	26	theme	B.	1111:1112	arg1	pre-treatment					1134:1146	B. bifidum CGMCC 15068 pre-treatment	1111:1146	B. bifidum CGMCC 15068 pre-treatment	1111:1146	In addition, B. bifidum CGMCC 15068 pre-treatment increased the relative abundance of Akkermansia, Desulfovibrionaceae, Romboutsia, Turicibacter, Verrucomicrobiaceae, Ruminococcaceae_UCG_013, Lachnospiraceae_UCG_004, and Lactobacillus.
32367312	1	27	theme	probiotics	287:296	arg1	use					280:282	the use	276:282	the use of probiotics	276:296	The gut microbiota plays an important role in colorectal cancer (CRC), and the use of probiotics might be a promising intervention method.
32367312	1	27	theme	probiotics	287:296	arg1	method					332:337	a promising intervention method	307:337	a promising intervention method	307:337	The gut microbiota plays an important role in colorectal cancer (CRC), and the use of probiotics might be a promising intervention method.
32367312	5	28	theme	microbial	847:855	arg1	composition					857:867	the faecal microbial composition	836:867	the faecal microbial composition	836:867	Then, the faecal microbial composition and metabolome were profiled using the 16S rRNA sequencing technology and gas chromatography-mass spectrometry (GC-MS), respectively.
32367312	2	29	from	effect	395:400	arg1	model					539:543	an azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated CRC (CAC) mouse model	444:543	an azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated CRC (CAC) mouse model	444:543	The aim of our study was to investigate the beneficial effect of Bifidobacterium bifidum CGMCC 15068 on an azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated CRC (CAC) mouse model.
32367312	1	30	theme	promising	309:317	arg1	method					332:337	a promising intervention method	307:337	a promising intervention method	307:337	The gut microbiota plays an important role in colorectal cancer (CRC), and the use of probiotics might be a promising intervention method.
32367312	1	30	theme	promising	309:317	arg1	use					280:282	the use	276:282	the use of probiotics	276:296	The gut microbiota plays an important role in colorectal cancer (CRC), and the use of probiotics might be a promising intervention method.
32367312	7	31	theme	Verrucomicrobiaceae	1244:1262	arg1	abundance					1171:1179	the relative abundance	1158:1179	the relative abundance of Akkermansia, Desulfovibrionaceae, Romboutsia, Turicibacter, Verrucomicrobiaceae, Ruminococcaceae_UCG_013, Lachnospiraceae_UCG_004, and Lactobacillus	1158:1331	In addition, B. bifidum CGMCC 15068 pre-treatment increased the relative abundance of Akkermansia, Desulfovibrionaceae, Romboutsia, Turicibacter, Verrucomicrobiaceae, Ruminococcaceae_UCG_013, Lachnospiraceae_UCG_004, and Lactobacillus.
32367312	2	32	theme	beneficial	384:393	arg1	effect					395:400	the beneficial effect	380:400	the beneficial effect of Bifidobacterium bifidum CGMCC 15068 on an azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated CRC (CAC) mouse model	380:543	The aim of our study was to investigate the beneficial effect of Bifidobacterium bifidum CGMCC 15068 on an azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated CRC (CAC) mouse model.
32367312	11	33	theme	prevention	1861:1870	arg1	strategy					1872:1879	a promising prevention strategy	1849:1879	a promising prevention strategy for CRC	1849:1887	The manipulation of the gut microbial composition using probiotics might be a promising prevention strategy for CRC.
32367312	11	33	theme	prevention	1861:1870	arg1	manipulation					1777:1788	The manipulation	1773:1788	The manipulation of the gut microbial composition using probiotics	1773:1838	The manipulation of the gut microbial composition using probiotics might be a promising prevention strategy for CRC.
32367312	1	34	theme	colorectal	247:256	arg1	CRC					266:268	CRC	266:268	CRC	266:268	The gut microbiota plays an important role in colorectal cancer (CRC), and the use of probiotics might be a promising intervention method.
32367312	1	34	theme	colorectal	247:256	arg1	cancer					258:263	colorectal cancer	247:263	colorectal cancer (CRC)	247:269	The gut microbiota plays an important role in colorectal cancer (CRC), and the use of probiotics might be a promising intervention method.
32367312	7	35	theme	Turicibacter	1230:1241	arg1	abundance					1171:1179	the relative abundance	1158:1179	the relative abundance of Akkermansia, Desulfovibrionaceae, Romboutsia, Turicibacter, Verrucomicrobiaceae, Ruminococcaceae_UCG_013, Lachnospiraceae_UCG_004, and Lactobacillus	1158:1331	In addition, B. bifidum CGMCC 15068 pre-treatment increased the relative abundance of Akkermansia, Desulfovibrionaceae, Romboutsia, Turicibacter, Verrucomicrobiaceae, Ruminococcaceae_UCG_013, Lachnospiraceae_UCG_004, and Lactobacillus.
32367312	8	36	theme	acid	1470:1473	arg1	biosynthesis					1475:1486	fatty acid biosynthesis	1464:1486	fatty acid biosynthesis	1464:1486	Meanwhile, B. bifidum CGMCC 15068 altered metabolites involved in the citrate cycle (TCA cycle), glycolysis, butyrate metabolism, fatty acid biosynthesis, and galactose metabolism.
32367312	8	36	theme	acid	1470:1473	arg1	cycle					1412:1416	the citrate cycle	1400:1416	the citrate cycle (TCA cycle)	1400:1428	Meanwhile, B. bifidum CGMCC 15068 altered metabolites involved in the citrate cycle (TCA cycle), glycolysis, butyrate metabolism, fatty acid biosynthesis, and galactose metabolism.
32367312	0	37	theme	Bifidobacterium	18:32	arg1	CGMCC					42:46	Bifidobacterium bifidum CGMCC 15068	18:52	Bifidobacterium bifidum CGMCC 15068	18:52	Administration of Bifidobacterium bifidum CGMCC 15068 modulates gut microbiota and metabolome in azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated colon cancer (CAC) in mice.
32367312	9	38	theme	Several	1515:1521	arg1	correlations					1535:1546	Several significant correlations	1515:1546	Several significant correlations	1515:1546	Several significant correlations were identified between the differentially abundant microbes and metabolites.
32367312	2	39	theme	bifidum	421:427	arg1	15068					435:439	Bifidobacterium bifidum CGMCC 15068	405:439	Bifidobacterium bifidum CGMCC 15068	405:439	The aim of our study was to investigate the beneficial effect of Bifidobacterium bifidum CGMCC 15068 on an azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated CRC (CAC) mouse model.
32367312	0	40	theme	CGMCC	42:46	arg1	Administration					0:13	Administration	0:13	Administration of Bifidobacterium bifidum CGMCC 15068	0:52	Administration of Bifidobacterium bifidum CGMCC 15068 modulates gut microbiota and metabolome in azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated colon cancer (CAC) in mice.
32367312	5	41	theme	16S	908:910	arg1	sequencing					917:926	the 16S rRNA sequencing	904:926	the 16S rRNA sequencing technology	904:937	Then, the faecal microbial composition and metabolome were profiled using the 16S rRNA sequencing technology and gas chromatography-mass spectrometry (GC-MS), respectively.
32367312	7	42	theme	Desulfovibrionaceae	1197:1215	arg1	abundance					1171:1179	the relative abundance	1158:1179	the relative abundance of Akkermansia, Desulfovibrionaceae, Romboutsia, Turicibacter, Verrucomicrobiaceae, Ruminococcaceae_UCG_013, Lachnospiraceae_UCG_004, and Lactobacillus	1158:1331	In addition, B. bifidum CGMCC 15068 pre-treatment increased the relative abundance of Akkermansia, Desulfovibrionaceae, Romboutsia, Turicibacter, Verrucomicrobiaceae, Ruminococcaceae_UCG_013, Lachnospiraceae_UCG_004, and Lactobacillus.
32367312	8	43	theme	galactose	1493:1501	arg1	metabolism					1503:1512	galactose metabolism	1493:1512	galactose metabolism	1493:1512	Meanwhile, B. bifidum CGMCC 15068 altered metabolites involved in the citrate cycle (TCA cycle), glycolysis, butyrate metabolism, fatty acid biosynthesis, and galactose metabolism.
32367312	8	43	theme	galactose	1493:1501	arg1	cycle					1412:1416	the citrate cycle	1400:1416	the citrate cycle (TCA cycle)	1400:1428	Meanwhile, B. bifidum CGMCC 15068 altered metabolites involved in the citrate cycle (TCA cycle), glycolysis, butyrate metabolism, fatty acid biosynthesis, and galactose metabolism.
32367312	8	44	theme	bifidum	1348:1354	arg1	15068					1362:1366	B. bifidum CGMCC 15068	1345:1366	B. bifidum CGMCC 15068	1345:1366	Meanwhile, B. bifidum CGMCC 15068 altered metabolites involved in the citrate cycle (TCA cycle), glycolysis, butyrate metabolism, fatty acid biosynthesis, and galactose metabolism.
32367312	8	44	theme	bifidum	1348:1354	arg1	Meanwhile					1334:1342	Meanwhile	1334:1342	Meanwhile	1334:1342	Meanwhile, B. bifidum CGMCC 15068 altered metabolites involved in the citrate cycle (TCA cycle), glycolysis, butyrate metabolism, fatty acid biosynthesis, and galactose metabolism.
32367312	10	45	theme	B.	1674:1675	arg1	15068					1691:1695	B. bifidum CGMCC 15068	1674:1695	B. bifidum CGMCC 15068	1674:1695	These findings supported the beneficial role of B. bifidum CGMCC 15068 in intestinal health by modulating dysbiosis and the gut metabolic profile.
32367312	6	46	theme	B.	1025:1026	arg1	15068					1042:1046	B. bifidum CGMCC 15068	1025:1046	B. bifidum CGMCC 15068	1025:1046	The administration of B. bifidum CGMCC 15068 attenuated tumourigenesis in the CAC mouse model.
32367312	3	47	theme	AOM	598:600	arg1	injection					585:593	an intra-peritoneal injection	565:593	an intra-peritoneal injection of AOM (10 mg/kg)	565:611	CAC was induced by an intra-peritoneal injection of AOM (10 mg/kg) and three 7-day cycles of 2% DSS in drinking water with a 14-day recovery period between two consecutive DSS administrations.
32367312	3	47	theme	AOM	598:600	arg1	cycles					629:634	three 7-day cycles	617:634	three 7-day cycles of 2% DSS in drinking water with a 14-day recovery period between two consecutive DSS administrations	617:736	CAC was induced by an intra-peritoneal injection of AOM (10 mg/kg) and three 7-day cycles of 2% DSS in drinking water with a 14-day recovery period between two consecutive DSS administrations.
32367312	11	48	theme	microbial	1801:1809	arg1	composition					1811:1821	the gut microbial composition	1793:1821	the gut microbial composition using probiotics	1793:1838	The manipulation of the gut microbial composition using probiotics might be a promising prevention strategy for CRC.
32367312	9	49	theme	abundant	1591:1598	arg1	microbes					1600:1607	microbes	1600:1607	microbes	1600:1607	Several significant correlations were identified between the differentially abundant microbes and metabolites.
32367312	10	50	theme	CGMCC	1685:1689	arg1	15068					1691:1695	B. bifidum CGMCC 15068	1674:1695	B. bifidum CGMCC 15068	1674:1695	These findings supported the beneficial role of B. bifidum CGMCC 15068 in intestinal health by modulating dysbiosis and the gut metabolic profile.
32367312	2	51	theme	CAC	528:530	arg1	model					539:543	an azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated CRC (CAC) mouse model	444:543	an azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated CRC (CAC) mouse model	444:543	The aim of our study was to investigate the beneficial effect of Bifidobacterium bifidum CGMCC 15068 on an azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated CRC (CAC) mouse model.
32367312	10	52	from	role	1666:1669	arg1	health					1711:1716	intestinal health	1700:1716	intestinal health	1700:1716	These findings supported the beneficial role of B. bifidum CGMCC 15068 in intestinal health by modulating dysbiosis and the gut metabolic profile.
32367312	2	53	theme	CRC	523:525	arg1	model					539:543	an azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated CRC (CAC) mouse model	444:543	an azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated CRC (CAC) mouse model	444:543	The aim of our study was to investigate the beneficial effect of Bifidobacterium bifidum CGMCC 15068 on an azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated CRC (CAC) mouse model.
32367312	6	54	theme	CAC	1081:1083	arg1	model					1091:1095	the CAC mouse model	1077:1095	the CAC mouse model	1077:1095	The administration of B. bifidum CGMCC 15068 attenuated tumourigenesis in the CAC mouse model.
32367312	5	55	dep	sequencing	917:926	arg1	technology					928:937	technology	928:937	the 16S rRNA sequencing technology	904:937	Then, the faecal microbial composition and metabolome were profiled using the 16S rRNA sequencing technology and gas chromatography-mass spectrometry (GC-MS), respectively.
32367312	3	56	theme	7-day	623:627	arg1	cycles					629:634	three 7-day cycles	617:634	three 7-day cycles of 2% DSS in drinking water with a 14-day recovery period between two consecutive DSS administrations	617:736	CAC was induced by an intra-peritoneal injection of AOM (10 mg/kg) and three 7-day cycles of 2% DSS in drinking water with a 14-day recovery period between two consecutive DSS administrations.
32367312	10	57	theme	gut	1750:1752	arg1	profile					1764:1770	the gut metabolic profile	1746:1770	the gut metabolic profile	1746:1770	These findings supported the beneficial role of B. bifidum CGMCC 15068 in intestinal health by modulating dysbiosis and the gut metabolic profile.
32367312	2	58	theme	mouse	533:537	arg1	model					539:543	an azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated CRC (CAC) mouse model	444:543	an azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated CRC (CAC) mouse model	444:543	The aim of our study was to investigate the beneficial effect of Bifidobacterium bifidum CGMCC 15068 on an azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated CRC (CAC) mouse model.
32367312	11	59	theme	composition	1811:1821	arg1	strategy					1872:1879	a promising prevention strategy	1849:1879	a promising prevention strategy for CRC	1849:1887	The manipulation of the gut microbial composition using probiotics might be a promising prevention strategy for CRC.
32367312	11	59	theme	composition	1811:1821	arg1	manipulation					1777:1788	The manipulation	1773:1788	The manipulation of the gut microbial composition using probiotics	1773:1838	The manipulation of the gut microbial composition using probiotics might be a promising prevention strategy for CRC.
32367312	12	60	theme	potential	1954:1962	arg1	applications					1973:1984	the potential clinical applications	1950:1984	the potential clinical applications of this strategy in the future	1950:2015	Long-term and large-scale clinical trials are warranted for the potential clinical applications of this strategy in the future.
32367312	6	61	theme	CGMCC	1036:1040	arg1	15068					1042:1046	B. bifidum CGMCC 15068	1025:1046	B. bifidum CGMCC 15068	1025:1046	The administration of B. bifidum CGMCC 15068 attenuated tumourigenesis in the CAC mouse model.
32367312	3	62	theme	%	640:640	arg1	DSS					642:644	2% DSS	639:644	2% DSS in drinking water with a 14-day recovery period between two consecutive DSS administrations	639:736	CAC was induced by an intra-peritoneal injection of AOM (10 mg/kg) and three 7-day cycles of 2% DSS in drinking water with a 14-day recovery period between two consecutive DSS administrations.
32367312	12	63	theme	clinical	1916:1923	arg1	trials					1925:1930	Long-term and large-scale clinical trials	1890:1930	trials	1925:1930	Long-term and large-scale clinical trials are warranted for the potential clinical applications of this strategy in the future.
32367312	4	64	theme	recovery	813:820	arg1	period					822:827	the recovery period	809:827	the recovery period	809:827	B. bifidum CGMCC 15068 (3 × 109 CFU/mL) was gavaged once daily during the recovery period.
32367312	8	65	theme	citrate	1404:1410	arg1	metabolism					1452:1461	butyrate metabolism	1443:1461	butyrate metabolism	1443:1461	Meanwhile, B. bifidum CGMCC 15068 altered metabolites involved in the citrate cycle (TCA cycle), glycolysis, butyrate metabolism, fatty acid biosynthesis, and galactose metabolism.
32367312	8	65	theme	citrate	1404:1410	arg1	metabolism					1503:1512	galactose metabolism	1493:1512	galactose metabolism	1493:1512	Meanwhile, B. bifidum CGMCC 15068 altered metabolites involved in the citrate cycle (TCA cycle), glycolysis, butyrate metabolism, fatty acid biosynthesis, and galactose metabolism.
32367312	8	65	theme	citrate	1404:1410	arg1	glycolysis					1431:1440	glycolysis	1431:1440	glycolysis	1431:1440	Meanwhile, B. bifidum CGMCC 15068 altered metabolites involved in the citrate cycle (TCA cycle), glycolysis, butyrate metabolism, fatty acid biosynthesis, and galactose metabolism.
32367312	8	65	theme	citrate	1404:1410	arg1	biosynthesis					1475:1486	fatty acid biosynthesis	1464:1486	fatty acid biosynthesis	1464:1486	Meanwhile, B. bifidum CGMCC 15068 altered metabolites involved in the citrate cycle (TCA cycle), glycolysis, butyrate metabolism, fatty acid biosynthesis, and galactose metabolism.
32367312	8	65	theme	citrate	1404:1410	arg1	cycle					1412:1416	the citrate cycle	1400:1416	the citrate cycle (TCA cycle)	1400:1428	Meanwhile, B. bifidum CGMCC 15068 altered metabolites involved in the citrate cycle (TCA cycle), glycolysis, butyrate metabolism, fatty acid biosynthesis, and galactose metabolism.
32367312	8	65	theme	citrate	1404:1410	arg1	cycle					1423:1427	TCA cycle	1419:1427	TCA cycle	1419:1427	Meanwhile, B. bifidum CGMCC 15068 altered metabolites involved in the citrate cycle (TCA cycle), glycolysis, butyrate metabolism, fatty acid biosynthesis, and galactose metabolism.
32367312	11	66	theme	gut	1797:1799	arg1	composition					1811:1821	the gut microbial composition	1793:1821	the gut microbial composition using probiotics	1793:1838	The manipulation of the gut microbial composition using probiotics might be a promising prevention strategy for CRC.
32367312	2	67	theme	-induced	495:502	arg1	model					539:543	an azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated CRC (CAC) mouse model	444:543	an azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated CRC (CAC) mouse model	444:543	The aim of our study was to investigate the beneficial effect of Bifidobacterium bifidum CGMCC 15068 on an azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated CRC (CAC) mouse model.
32367312	0	68	theme	colitis-associated	154:171	arg1	CAC					187:189	CAC	187:189	CAC	187:189	Administration of Bifidobacterium bifidum CGMCC 15068 modulates gut microbiota and metabolome in azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated colon cancer (CAC) in mice.
32367312	0	68	theme	colitis-associated	154:171	arg1	cancer					179:184	azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated colon cancer (CAC)	97:190	azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated colon cancer (CAC)	97:190	Administration of Bifidobacterium bifidum CGMCC 15068 modulates gut microbiota and metabolome in azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated colon cancer (CAC) in mice.
32367312	7	69	theme	Lachnospiraceae_UCG_004	1290:1312	arg1	abundance					1171:1179	the relative abundance	1158:1179	the relative abundance of Akkermansia, Desulfovibrionaceae, Romboutsia, Turicibacter, Verrucomicrobiaceae, Ruminococcaceae_UCG_013, Lachnospiraceae_UCG_004, and Lactobacillus	1158:1331	In addition, B. bifidum CGMCC 15068 pre-treatment increased the relative abundance of Akkermansia, Desulfovibrionaceae, Romboutsia, Turicibacter, Verrucomicrobiaceae, Ruminococcaceae_UCG_013, Lachnospiraceae_UCG_004, and Lactobacillus.
32367312	5	70	theme	chromatography-mass	947:965	arg1	GC-MS					981:985	GC-MS	981:985	GC-MS	981:985	Then, the faecal microbial composition and metabolome were profiled using the 16S rRNA sequencing technology and gas chromatography-mass spectrometry (GC-MS), respectively.
32367312	5	70	theme	chromatography-mass	947:965	arg1	spectrometry					967:978	gas chromatography-mass spectrometry	943:978	gas chromatography-mass spectrometry (GC-MS)	943:986	Then, the faecal microbial composition and metabolome were profiled using the 16S rRNA sequencing technology and gas chromatography-mass spectrometry (GC-MS), respectively.
32367312	7	71	theme	Ruminococcaceae_UCG_013	1265:1287	arg1	abundance					1171:1179	the relative abundance	1158:1179	the relative abundance of Akkermansia, Desulfovibrionaceae, Romboutsia, Turicibacter, Verrucomicrobiaceae, Ruminococcaceae_UCG_013, Lachnospiraceae_UCG_004, and Lactobacillus	1158:1331	In addition, B. bifidum CGMCC 15068 pre-treatment increased the relative abundance of Akkermansia, Desulfovibrionaceae, Romboutsia, Turicibacter, Verrucomicrobiaceae, Ruminococcaceae_UCG_013, Lachnospiraceae_UCG_004, and Lactobacillus.
32367312	3	72	theme	recovery	678:685	arg1	period					687:692	a 14-day recovery period	669:692	a 14-day recovery period between two consecutive DSS administrations	669:736	CAC was induced by an intra-peritoneal injection of AOM (10 mg/kg) and three 7-day cycles of 2% DSS in drinking water with a 14-day recovery period between two consecutive DSS administrations.
32367312	12	73	theme	Long-term	1890:1898	arg1	trials					1925:1930	Long-term and large-scale clinical trials	1890:1930	trials	1925:1930	Long-term and large-scale clinical trials are warranted for the potential clinical applications of this strategy in the future.
32367312	2	74	theme	CGMCC	429:433	arg1	15068					435:439	Bifidobacterium bifidum CGMCC 15068	405:439	Bifidobacterium bifidum CGMCC 15068	405:439	The aim of our study was to investigate the beneficial effect of Bifidobacterium bifidum CGMCC 15068 on an azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated CRC (CAC) mouse model.
32367312	12	75	theme	large-scale	1904:1914	arg1	trials					1925:1930	Long-term and large-scale clinical trials	1890:1930	trials	1925:1930	Long-term and large-scale clinical trials are warranted for the potential clinical applications of this strategy in the future.
32367312	5	76	theme	faecal	840:845	arg1	composition					857:867	the faecal microbial composition	836:867	the faecal microbial composition	836:867	Then, the faecal microbial composition and metabolome were profiled using the 16S rRNA sequencing technology and gas chromatography-mass spectrometry (GC-MS), respectively.
32367312	3	77	theme	consecutive	706:716	arg1	administrations					722:736	two consecutive DSS administrations	702:736	two consecutive DSS administrations	702:736	CAC was induced by an intra-peritoneal injection of AOM (10 mg/kg) and three 7-day cycles of 2% DSS in drinking water with a 14-day recovery period between two consecutive DSS administrations.
32367312	8	78	theme	fatty	1464:1468	arg1	biosynthesis					1475:1486	fatty acid biosynthesis	1464:1486	fatty acid biosynthesis	1464:1486	Meanwhile, B. bifidum CGMCC 15068 altered metabolites involved in the citrate cycle (TCA cycle), glycolysis, butyrate metabolism, fatty acid biosynthesis, and galactose metabolism.
32367312	8	78	theme	fatty	1464:1468	arg1	cycle					1412:1416	the citrate cycle	1400:1416	the citrate cycle (TCA cycle)	1400:1428	Meanwhile, B. bifidum CGMCC 15068 altered metabolites involved in the citrate cycle (TCA cycle), glycolysis, butyrate metabolism, fatty acid biosynthesis, and galactose metabolism.
32367312	12	79	dep	potential	1954:1962	arg1	clinical					1964:1971	clinical	1964:1971	clinical	1964:1971	Long-term and large-scale clinical trials are warranted for the potential clinical applications of this strategy in the future.
32367312	1	80	theme	intervention	319:330	arg1	method					332:337	a promising intervention method	307:337	a promising intervention method	307:337	The gut microbiota plays an important role in colorectal cancer (CRC), and the use of probiotics might be a promising intervention method.
32367312	1	80	theme	intervention	319:330	arg1	use					280:282	the use	276:282	the use of probiotics	276:296	The gut microbiota plays an important role in colorectal cancer (CRC), and the use of probiotics might be a promising intervention method.
32367312	8	81	theme	butyrate	1443:1450	arg1	metabolism					1452:1461	butyrate metabolism	1443:1461	butyrate metabolism	1443:1461	Meanwhile, B. bifidum CGMCC 15068 altered metabolites involved in the citrate cycle (TCA cycle), glycolysis, butyrate metabolism, fatty acid biosynthesis, and galactose metabolism.
32367312	8	81	theme	butyrate	1443:1450	arg1	cycle					1412:1416	the citrate cycle	1400:1416	the citrate cycle (TCA cycle)	1400:1428	Meanwhile, B. bifidum CGMCC 15068 altered metabolites involved in the citrate cycle (TCA cycle), glycolysis, butyrate metabolism, fatty acid biosynthesis, and galactose metabolism.
32367312	9	82	dep	microbes	1600:1607	arg1	the					1572:1574	the	1572:1574	the	1572:1574	Several significant correlations were identified between the differentially abundant microbes and metabolites.
32367312	7	83	dep	B.	1111:1112	arg1	bifidum					1114:1120	bifidum	1114:1120	bifidum	1114:1120	In addition, B. bifidum CGMCC 15068 pre-treatment increased the relative abundance of Akkermansia, Desulfovibrionaceae, Romboutsia, Turicibacter, Verrucomicrobiaceae, Ruminococcaceae_UCG_013, Lachnospiraceae_UCG_004, and Lactobacillus.
32367312	2	84	theme	study	355:359	arg1	aim					344:346	The aim	340:346	The aim of our study	340:359	The aim of our study was to investigate the beneficial effect of Bifidobacterium bifidum CGMCC 15068 on an azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated CRC (CAC) mouse model.
32367312	9	85	theme	significant	1523:1533	arg1	correlations					1535:1546	Several significant correlations	1515:1546	Several significant correlations	1515:1546	Several significant correlations were identified between the differentially abundant microbes and metabolites.
32367312	2	86	theme	Bifidobacterium	405:419	arg1	15068					435:439	Bifidobacterium bifidum CGMCC 15068	405:439	Bifidobacterium bifidum CGMCC 15068	405:439	The aim of our study was to investigate the beneficial effect of Bifidobacterium bifidum CGMCC 15068 on an azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated CRC (CAC) mouse model.
32367312	0	87	theme	gut	64:66	arg1	microbiota					68:77	gut microbiota	64:77	gut microbiota	64:77	Administration of Bifidobacterium bifidum CGMCC 15068 modulates gut microbiota and metabolome in azoxymethane (AOM)/dextran sulphate sodium (DSS)-induced colitis-associated colon cancer (CAC) in mice.
32367312	5	88	theme	rRNA	912:915	arg1	sequencing					917:926	the 16S rRNA sequencing	904:926	the 16S rRNA sequencing technology	904:937	Then, the faecal microbial composition and metabolome were profiled using the 16S rRNA sequencing technology and gas chromatography-mass spectrometry (GC-MS), respectively.
32367312	4	89	theme	bifidum	742:748	arg1	3 × 109 CFU/mL					763:776	3 × 109 CFU/mL	763:776	3 × 109 CFU/mL	763:776	B. bifidum CGMCC 15068 (3 × 109 CFU/mL) was gavaged once daily during the recovery period.
32367312	4	89	theme	bifidum	742:748	arg1	15068					756:760	B. bifidum CGMCC 15068	739:760	B. bifidum CGMCC 15068 (3 × 109 CFU/mL)	739:777	B. bifidum CGMCC 15068 (3 × 109 CFU/mL) was gavaged once daily during the recovery period.
32367312	6	90	theme	bifidum	1028:1034	arg1	15068					1042:1046	B. bifidum CGMCC 15068	1025:1046	B. bifidum CGMCC 15068	1025:1046	The administration of B. bifidum CGMCC 15068 attenuated tumourigenesis in the CAC mouse model.
32367312	8	91	theme	B.	1345:1346	arg1	15068					1362:1366	B. bifidum CGMCC 15068	1345:1366	B. bifidum CGMCC 15068	1345:1366	Meanwhile, B. bifidum CGMCC 15068 altered metabolites involved in the citrate cycle (TCA cycle), glycolysis, butyrate metabolism, fatty acid biosynthesis, and galactose metabolism.
32367312	8	91	theme	B.	1345:1346	arg1	Meanwhile					1334:1342	Meanwhile	1334:1342	Meanwhile	1334:1342	Meanwhile, B. bifidum CGMCC 15068 altered metabolites involved in the citrate cycle (TCA cycle), glycolysis, butyrate metabolism, fatty acid biosynthesis, and galactose metabolism.
32367312	7	92	theme	relative	1162:1169	arg1	abundance					1171:1179	the relative abundance	1158:1179	the relative abundance of Akkermansia, Desulfovibrionaceae, Romboutsia, Turicibacter, Verrucomicrobiaceae, Ruminococcaceae_UCG_013, Lachnospiraceae_UCG_004, and Lactobacillus	1158:1331	In addition, B. bifidum CGMCC 15068 pre-treatment increased the relative abundance of Akkermansia, Desulfovibrionaceae, Romboutsia, Turicibacter, Verrucomicrobiaceae, Ruminococcaceae_UCG_013, Lachnospiraceae_UCG_004, and Lactobacillus.
32367312	3	93	theme	intra-peritoneal	568:583	arg1	injection					585:593	an intra-peritoneal injection	565:593	an intra-peritoneal injection of AOM (10 mg/kg)	565:611	CAC was induced by an intra-peritoneal injection of AOM (10 mg/kg) and three 7-day cycles of 2% DSS in drinking water with a 14-day recovery period between two consecutive DSS administrations.
32367312	8	94	theme	CGMCC	1356:1360	arg1	15068					1362:1366	B. bifidum CGMCC 15068	1345:1366	B. bifidum CGMCC 15068	1345:1366	Meanwhile, B. bifidum CGMCC 15068 altered metabolites involved in the citrate cycle (TCA cycle), glycolysis, butyrate metabolism, fatty acid biosynthesis, and galactose metabolism.
32367312	8	94	theme	CGMCC	1356:1360	arg1	Meanwhile					1334:1342	Meanwhile	1334:1342	Meanwhile	1334:1342	Meanwhile, B. bifidum CGMCC 15068 altered metabolites involved in the citrate cycle (TCA cycle), glycolysis, butyrate metabolism, fatty acid biosynthesis, and galactose metabolism.
32367312	10	95	theme	beneficial	1655:1664	arg1	role					1666:1669	the beneficial role	1651:1669	the beneficial role of B. bifidum CGMCC 15068 in intestinal health	1651:1716	These findings supported the beneficial role of B. bifidum CGMCC 15068 in intestinal health by modulating dysbiosis and the gut metabolic profile.
32367312	1	96	theme	gut	205:207	arg1	microbiota					209:218	The gut microbiota	201:218	The gut microbiota	201:218	The gut microbiota plays an important role in colorectal cancer (CRC), and the use of probiotics might be a promising intervention method.
32367312	10	97	theme	15068	1691:1695	arg1	role					1666:1669	the beneficial role	1651:1669	the beneficial role of B. bifidum CGMCC 15068 in intestinal health	1651:1716	These findings supported the beneficial role of B. bifidum CGMCC 15068 in intestinal health by modulating dysbiosis and the gut metabolic profile.
32229124	20	0	theme	mastitis	3081:3088	arg1	plans					3101:3105	mastitis monitoring plans	3081:3105	mastitis monitoring plans aiming to reduce SCC and udder issues at both the individual and farm levels in the Italian buffalo population	3081:3216	Finally, these results could be used in mastitis monitoring plans aiming to reduce SCC and udder issues at both the individual and farm levels in the Italian buffalo population.
32229124	2	1	theme	animal	491:496	arg1	welfare					498:504	better animal welfare	484:504	better animal welfare	484:504	Despite the economic boost of the last 2 decades, the buffalo farming system should be improved to maximize the efficiency of the dairy industry, improve yield and quality of milk and cheese, and work toward better animal welfare.
32229124	17	2	theme	cell	2675:2678	arg1	score					2680:2684	somatic cell score	2667:2684	somatic cell score	2667:2684	Significantly lower milk yield and lactose percentage were estimated in progressively higher classes of somatic cell score, whereas no significant differences were observed for fat and protein percentages.
32229124	3	3	theme	udder	574:578	arg1	health					580:585	udder health	574:585	udder health	574:585	Milk somatic cell count (SCC) is used worldwide as an indicator of udder health in individual milk and is useful for monitoring farm hygiene in bulk milk.
32229124	14	4	theme	lactations	2085:2094	arg1	proportion					2071:2080	the proportion	2067:2080	the proportion of lactations with average SCC ≥500,000 cells/mL	2067:2129	Supporting this, the proportion of lactations with average SCC ≥500,000 cells/mL increased from 6% in first parity to 12% in sixth parity.
32229124	10	5	dep	showed	1636:1641	arg1	i.e.					1679:1682	i.e.	1679:1682	i.e.	1679:1682	Data revealed that around 11% of herd-test-dates (≥5 animals sampled each) showed average milk SCC ≥400,000 cells/mL (i.e., above the threshold fixed by the European Community for bovine milk).
32229124	10	6	theme	fixed	1705:1709	arg1	threshold					1695:1703	the threshold	1691:1703	the threshold fixed by the European Community for bovine milk	1691:1751	Data revealed that around 11% of herd-test-dates (≥5 animals sampled each) showed average milk SCC ≥400,000 cells/mL (i.e., above the threshold fixed by the European Community for bovine milk).
32229124	20	7	used	used	3073:3076	arg2	results					3056:3062	these results	3050:3062	these results	3050:3062	Finally, these results could be used in mastitis monitoring plans aiming to reduce SCC and udder issues at both the individual and farm levels in the Italian buffalo population.
32229124	1	8	theme	Protected	229:237	arg1	Denomination					239:250	Mozzarella di Bufala Campana Protected Denomination	200:250	Mozzarella di Bufala Campana Protected Denomination of Origin (PDO) cheese	200:273	In Southern Italy, buffalo (Bubalus bubalis) milk is mostly intended for the manufacture of Mozzarella di Bufala Campana Protected Denomination of Origin (PDO) cheese.
32229124	14	9	theme	first	2152:2156	arg1	parity					2158:2163	first parity	2152:2163	first parity to 12% in sixth parity	2152:2186	Supporting this, the proportion of lactations with average SCC ≥500,000 cells/mL increased from 6% in first parity to 12% in sixth parity.
32229124	9	10	theme	milk	1464:1467	arg1	quality					1469:1475	buffalo milk quality	1456:1475	buffalo milk quality	1456:1475	To provide a phenotypic characterization of SCC at the population level and to improve knowledge on buffalo milk quality, 876,299 test-day records of 70,156 buffaloes reared in the PDO area were analyzed.
32229124	6	11	theme	delivered	1011:1019	arg1	volume					1021:1026	the delivered volume	1007:1026	the delivered volume of milk	1007:1034	However, payment systems of the PDO area are based simply on the delivered volume of milk rather than on quality characteristics.
32229124	17	12	theme	significant	2698:2708	arg1	differences					2710:2720	no significant differences	2695:2720	no significant differences	2695:2720	Significantly lower milk yield and lactose percentage were estimated in progressively higher classes of somatic cell score, whereas no significant differences were observed for fat and protein percentages.
32229124	1	13	theme	bubalis	144:150	arg1	milk					153:156	buffalo (Bubalus bubalis) milk	127:156	buffalo (Bubalus bubalis) milk	127:156	In Southern Italy, buffalo (Bubalus bubalis) milk is mostly intended for the manufacture of Mozzarella di Bufala Campana Protected Denomination of Origin (PDO) cheese.
32229124	20	14	theme	SCC	3124:3126	arg1	issues					3138:3143	SCC and udder issues	3124:3143	SCC and udder issues	3124:3143	Finally, these results could be used in mastitis monitoring plans aiming to reduce SCC and udder issues at both the individual and farm levels in the Italian buffalo population.
32229124	7	15	from	penalties	1106:1114	arg1	milk					1141:1144	bulk milk	1136:1144	bulk milk in the Italian buffalo dairy industry	1136:1182	Hence, currently there are no penalties for elevated SCC in bulk milk in the Italian buffalo dairy industry.
32229124	11	16	theme	individual	1824:1833	arg1	level					1835:1839	both the farm and individual level	1806:1839	level	1835:1839	This suggests that there is room to improve SCC at both the farm and individual level.
32229124	17	17	theme	score	2680:2684	arg1	classes					2656:2662	progressively higher classes	2635:2662	progressively higher classes of somatic cell score	2635:2684	Significantly lower milk yield and lactose percentage were estimated in progressively higher classes of somatic cell score, whereas no significant differences were observed for fat and protein percentages.
32229124	3	18	theme	farm	635:638	arg1	hygiene					640:646	farm hygiene	635:646	farm hygiene in bulk milk	635:659	Milk somatic cell count (SCC) is used worldwide as an indicator of udder health in individual milk and is useful for monitoring farm hygiene in bulk milk.
32229124	17	19	theme	higher	2649:2654	arg1	classes					2656:2662	progressively higher classes	2635:2662	progressively higher classes of somatic cell score	2635:2684	Significantly lower milk yield and lactose percentage were estimated in progressively higher classes of somatic cell score, whereas no significant differences were observed for fat and protein percentages.
32229124	20	20	theme	udder	3132:3136	arg1	issues					3138:3143	SCC and udder issues	3124:3143	SCC and udder issues	3124:3143	Finally, these results could be used in mastitis monitoring plans aiming to reduce SCC and udder issues at both the individual and farm levels in the Italian buffalo population.
32229124	9	21	theme	test-day	1486:1493	arg1	records					1495:1501	876,299 test-day records	1478:1501	876,299 test-day records of 70,156 buffaloes reared in the PDO area	1478:1544	To provide a phenotypic characterization of SCC at the population level and to improve knowledge on buffalo milk quality, 876,299 test-day records of 70,156 buffaloes reared in the PDO area were analyzed.
32229124	16	22	theme	stage	2407:2411	arg1	effects					2378:2384	fixed effects	2372:2384	fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions	2372:2490	An ANOVA was carried out on test-day record milk yield and composition traits, with fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions; buffalo, herd-test-date, and residual were considered random effects.
32229124	16	23	theme	month	2451:2455	arg1	effects					2378:2384	fixed effects	2372:2384	fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions	2372:2490	An ANOVA was carried out on test-day record milk yield and composition traits, with fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions; buffalo, herd-test-date, and residual were considered random effects.
32229124	15	24	theme	SCC	2268:2270	arg1	level					2272:2276	SCC level	2268:2276	SCC level	2268:2276	Milk yield and SCC were negatively correlated with each other, especially when SCC level was high.
32229124	4	25	theme	udder	793:797	arg1	infection					799:807	suspected udder infection	783:807	suspected udder infection	783:807	Mastitis data are currently not available on a large scale in Italy; thus, SCC is essential for identifying animals with suspected udder infection and inflammation.
32229124	8	26	theme	European	1250:1257	arg1	regulations					1269:1279	the European Community regulations	1246:1279	the European Community regulations	1246:1279	In addition, SCC for buffalo milk is not mentioned by either the European Community regulations or the PDO protocol, evidencing a lack of rules for the maximum SCC limit.
32229124	16	27	theme	lactation	2397:2405	arg1	stage					2407:2411	lactation stage	2397:2411	lactation stage	2397:2411	An ANOVA was carried out on test-day record milk yield and composition traits, with fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions; buffalo, herd-test-date, and residual were considered random effects.
32229124	12	28	theme	first	1849:1853	arg1	parity					1855:1860	first parity	1849:1860	first parity	1849:1860	Within first parity, more than 28 and 15% of lactations had average SCC ≥200,000 and ≥300,000 cells/mL, respectively.
32229124	5	29	theme	milk	940:943	arg1	composition					878:888	altered composition	870:888	altered composition	870:888	Moreover, high milk SCC is associated with altered composition and acidity, and poor technological properties of milk.
32229124	5	29	theme	milk	940:943	arg1	properties					926:935	poor technological properties	907:935	poor technological properties of milk	907:943	Moreover, high milk SCC is associated with altered composition and acidity, and poor technological properties of milk.
32229124	5	29	theme	milk	940:943	arg1	acidity					894:900	acidity	894:900	acidity	894:900	Moreover, high milk SCC is associated with altered composition and acidity, and poor technological properties of milk.
32229124	3	30	theme	somatic	512:518	arg1	cell					520:523	Milk somatic cell	507:523	Milk somatic cell count (SCC)	507:535	Milk somatic cell count (SCC) is used worldwide as an indicator of udder health in individual milk and is useful for monitoring farm hygiene in bulk milk.
32229124	9	31	theme	buffaloes	1513:1521	arg1	records					1495:1501	876,299 test-day records	1478:1501	876,299 test-day records of 70,156 buffaloes reared in the PDO area	1478:1544	To provide a phenotypic characterization of SCC at the population level and to improve knowledge on buffalo milk quality, 876,299 test-day records of 70,156 buffaloes reared in the PDO area were analyzed.
32229124	10	32	theme	bovine	1741:1746	arg1	milk					1748:1751	bovine milk	1741:1751	bovine milk	1741:1751	Data revealed that around 11% of herd-test-dates (≥5 animals sampled each) showed average milk SCC ≥400,000 cells/mL (i.e., above the threshold fixed by the European Community for bovine milk).
32229124	17	33	theme	lower	2577:2581	arg1	yield					2588:2592	Significantly lower milk yield	2563:2592	Significantly lower milk yield	2563:2592	Significantly lower milk yield and lactose percentage were estimated in progressively higher classes of somatic cell score, whereas no significant differences were observed for fat and protein percentages.
32229124	9	34	from	knowledge	1443:1451	arg1	quality					1469:1475	buffalo milk quality	1456:1475	buffalo milk quality	1456:1475	To provide a phenotypic characterization of SCC at the population level and to improve knowledge on buffalo milk quality, 876,299 test-day records of 70,156 buffaloes reared in the PDO area were analyzed.
32229124	19	35	theme	reliable	2910:2917	arg1	thresholds					2937:2946	reliable and effective SCC thresholds	2910:2946	reliable and effective SCC thresholds	2910:2946	These findings may be helpful for defining reliable and effective SCC thresholds to be adopted whenever specific penalties for high SCC are included in milk payment systems.
32229124	5	36	theme	poor	907:910	arg1	properties					926:935	poor technological properties	907:935	poor technological properties of milk	907:943	Moreover, high milk SCC is associated with altered composition and acidity, and poor technological properties of milk.
32229124	10	37	theme	≥5	1611:1612	arg1	animals					1614:1620	≥5 animals	1611:1620	≥5 animals sampled each	1611:1633	Data revealed that around 11% of herd-test-dates (≥5 animals sampled each) showed average milk SCC ≥400,000 cells/mL (i.e., above the threshold fixed by the European Community for bovine milk).
32229124	1	38	theme	Southern	111:118	arg1	Italy					120:124	Southern Italy	111:124	Southern Italy	111:124	In Southern Italy, buffalo (Bubalus bubalis) milk is mostly intended for the manufacture of Mozzarella di Bufala Campana Protected Denomination of Origin (PDO) cheese.
32229124	9	39	theme	PDO	1537:1539	arg1	area					1541:1544	the PDO area	1533:1544	the PDO area	1533:1544	To provide a phenotypic characterization of SCC at the population level and to improve knowledge on buffalo milk quality, 876,299 test-day records of 70,156 buffaloes reared in the PDO area were analyzed.
32229124	7	40	theme	dairy	1169:1173	arg1	industry					1175:1182	the Italian buffalo dairy industry	1149:1182	the Italian buffalo dairy industry	1149:1182	Hence, currently there are no penalties for elevated SCC in bulk milk in the Italian buffalo dairy industry.
32229124	0	41	theme	milk	50:53	arg1	yield					55:59	milk yield	50:59	milk yield	50:59	Milk somatic cell count and its relationship with milk yield and quality traits in Italian water buffaloes.
32229124	10	42	theme	herd-test-dates	1594:1608	arg1	%					1589:1589	around 11%	1580:1589	around 11% of herd-test-dates (≥5 animals sampled each)	1580:1634	Data revealed that around 11% of herd-test-dates (≥5 animals sampled each) showed average milk SCC ≥400,000 cells/mL (i.e., above the threshold fixed by the European Community for bovine milk).
32229124	10	42	theme	herd-test-dates	1594:1608	arg1	herd-test-dates					1594:1608	herd-test-dates	1594:1608	herd-test-dates (≥5 animals sampled each)	1594:1634	Data revealed that around 11% of herd-test-dates (≥5 animals sampled each) showed average milk SCC ≥400,000 cells/mL (i.e., above the threshold fixed by the European Community for bovine milk).
32229124	20	43	theme	individual	3157:3166	arg1	levels					3177:3182	both the individual and farm levels	3148:3182	levels	3177:3182	Finally, these results could be used in mastitis monitoring plans aiming to reduce SCC and udder issues at both the individual and farm levels in the Italian buffalo population.
32229124	2	44	theme	milk	451:454	arg1	quality					440:446	quality	440:446	quality	440:446	Despite the economic boost of the last 2 decades, the buffalo farming system should be improved to maximize the efficiency of the dairy industry, improve yield and quality of milk and cheese, and work toward better animal welfare.
32229124	2	44	theme	milk	451:454	arg1	yield					430:434	yield	430:434	yield	430:434	Despite the economic boost of the last 2 decades, the buffalo farming system should be improved to maximize the efficiency of the dairy industry, improve yield and quality of milk and cheese, and work toward better animal welfare.
32229124	19	45	theme	effective	2923:2931	arg1	thresholds					2937:2946	reliable and effective SCC thresholds	2910:2946	reliable and effective SCC thresholds	2910:2946	These findings may be helpful for defining reliable and effective SCC thresholds to be adopted whenever specific penalties for high SCC are included in milk payment systems.
32229124	20	46	theme	farm	3172:3175	arg1	levels					3177:3182	both the individual and farm levels	3148:3182	levels	3177:3182	Finally, these results could be used in mastitis monitoring plans aiming to reduce SCC and udder issues at both the individual and farm levels in the Italian buffalo population.
32229124	18	47	theme	buffaloes	2856:2864	arg1	set					2835:2837	a large data set	2822:2837	a large data set of Italian dairy buffaloes	2822:2864	This is the first attempt to investigate milk SCC in a large data set of Italian dairy buffaloes.
32229124	18	47	theme	buffaloes	2856:2864	arg1	buffaloes					2856:2864	Italian dairy buffaloes	2842:2864	Italian dairy buffaloes	2842:2864	This is the first attempt to investigate milk SCC in a large data set of Italian dairy buffaloes.
32229124	8	48	theme	rules	1323:1327	arg1	lack					1315:1318	a lack	1313:1318	a lack of rules for the maximum SCC limit	1313:1353	In addition, SCC for buffalo milk is not mentioned by either the European Community regulations or the PDO protocol, evidencing a lack of rules for the maximum SCC limit.
32229124	2	49	theme	buffalo	330:336	arg1	system					346:351	the buffalo farming system	326:351	the buffalo farming system	326:351	Despite the economic boost of the last 2 decades, the buffalo farming system should be improved to maximize the efficiency of the dairy industry, improve yield and quality of milk and cheese, and work toward better animal welfare.
32229124	0	50	theme	Italian	83:89	arg1	buffaloes					97:105	Italian water buffaloes	83:105	Italian water buffaloes	83:105	Milk somatic cell count and its relationship with milk yield and quality traits in Italian water buffaloes.
32229124	4	51	from	scale	715:719	arg1	Italy					724:728	Italy	724:728	Italy	724:728	Mastitis data are currently not available on a large scale in Italy; thus, SCC is essential for identifying animals with suspected udder infection and inflammation.
32229124	12	52	contain	had	1898:1900	arg1	lactations					1887:1896	lactations	1887:1896	lactations	1887:1896	Within first parity, more than 28 and 15% of lactations had average SCC ≥200,000 and ≥300,000 cells/mL, respectively.
32229124	12	52	contain	had	1898:1900	arg1	%					1882:1882	more than 28 and 15%	1863:1882	more than 28 and 15% of lactations	1863:1896	Within first parity, more than 28 and 15% of lactations had average SCC ≥200,000 and ≥300,000 cells/mL, respectively.
32229124	12	52	contain	had	1898:1900	arg2	cells/mL					1936:1943	≥300,000 cells/mL	1927:1943	≥300,000 cells/mL	1927:1943	Within first parity, more than 28 and 15% of lactations had average SCC ≥200,000 and ≥300,000 cells/mL, respectively.
32229124	12	52	contain	had	1898:1900	arg2	≥200,000					1914:1921	average SCC ≥200,000	1902:1921	average SCC ≥200,000	1902:1921	Within first parity, more than 28 and 15% of lactations had average SCC ≥200,000 and ≥300,000 cells/mL, respectively.
32229124	5	53	theme	milk	842:845	arg1	SCC					847:849	high milk SCC	837:849	high milk SCC	837:849	Moreover, high milk SCC is associated with altered composition and acidity, and poor technological properties of milk.
32229124	7	54	theme	buffalo	1161:1167	arg1	industry					1175:1182	the Italian buffalo dairy industry	1149:1182	the Italian buffalo dairy industry	1149:1182	Hence, currently there are no penalties for elevated SCC in bulk milk in the Italian buffalo dairy industry.
32229124	0	55	theme	Milk	0:3	arg1	count					18:22	Milk somatic cell count	0:22	Milk somatic cell count	0:22	Milk somatic cell count and its relationship with milk yield and quality traits in Italian water buffaloes.
32229124	1	56	theme	Denomination	239:250	arg1	manufacture					185:195	the manufacture	181:195	the manufacture of Mozzarella di Bufala Campana Protected Denomination of Origin (PDO) cheese	181:273	In Southern Italy, buffalo (Bubalus bubalis) milk is mostly intended for the manufacture of Mozzarella di Bufala Campana Protected Denomination of Origin (PDO) cheese.
32229124	17	57	theme	lactose	2598:2604	arg1	percentage					2606:2615	lactose percentage	2598:2615	lactose percentage	2598:2615	Significantly lower milk yield and lactose percentage were estimated in progressively higher classes of somatic cell score, whereas no significant differences were observed for fat and protein percentages.
32229124	8	58	theme	SCC	1345:1347	arg1	limit					1349:1353	the maximum SCC limit	1333:1353	the maximum SCC limit	1333:1353	In addition, SCC for buffalo milk is not mentioned by either the European Community regulations or the PDO protocol, evidencing a lack of rules for the maximum SCC limit.
32229124	12	59	theme	≥300,000	1927:1934	arg1	cells/mL					1936:1943	≥300,000 cells/mL	1927:1943	≥300,000 cells/mL	1927:1943	Within first parity, more than 28 and 15% of lactations had average SCC ≥200,000 and ≥300,000 cells/mL, respectively.
32229124	18	60	theme	first	2781:2785	arg1	This					2769:2772	This	2769:2772	This	2769:2772	This is the first attempt to investigate milk SCC in a large data set of Italian dairy buffaloes.
32229124	18	60	theme	first	2781:2785	arg1	attempt					2787:2793	the first attempt	2777:2793	the first attempt to investigate milk SCC in a large data set of Italian dairy buffaloes	2777:2864	This is the first attempt to investigate milk SCC in a large data set of Italian dairy buffaloes.
32229124	0	61	theme	cell	13:16	arg1	count					18:22	Milk somatic cell count	0:22	Milk somatic cell count	0:22	Milk somatic cell count and its relationship with milk yield and quality traits in Italian water buffaloes.
32229124	1	62	theme	Origin	255:260	arg1	cheese					268:273	Origin (PDO) cheese	255:273	Origin (PDO) cheese	255:273	In Southern Italy, buffalo (Bubalus bubalis) milk is mostly intended for the manufacture of Mozzarella di Bufala Campana Protected Denomination of Origin (PDO) cheese.
32229124	16	63	theme	=	2445:2445	arg1	n					2443:2443	n = 6	2443:2447	n = 6	2443:2447	An ANOVA was carried out on test-day record milk yield and composition traits, with fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions; buffalo, herd-test-date, and residual were considered random effects.
32229124	16	63	theme	=	2445:2445	arg1	score					2436:2440	somatic cell score	2423:2440	somatic cell score (n = 6)	2423:2448	An ANOVA was carried out on test-day record milk yield and composition traits, with fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions; buffalo, herd-test-date, and residual were considered random effects.
32229124	18	64	theme	data	2830:2833	arg1	set					2835:2837	a large data set	2822:2837	a large data set of Italian dairy buffaloes	2822:2864	This is the first attempt to investigate milk SCC in a large data set of Italian dairy buffaloes.
32229124	18	64	theme	data	2830:2833	arg1	buffaloes					2856:2864	Italian dairy buffaloes	2842:2864	Italian dairy buffaloes	2842:2864	This is the first attempt to investigate milk SCC in a large data set of Italian dairy buffaloes.
32229124	16	65	theme	fixed	2372:2376	arg1	effects					2378:2384	fixed effects	2372:2384	fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions	2372:2490	An ANOVA was carried out on test-day record milk yield and composition traits, with fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions; buffalo, herd-test-date, and residual were considered random effects.
32229124	2	66	theme	dairy	406:410	arg1	industry					412:419	the dairy industry	402:419	the dairy industry	402:419	Despite the economic boost of the last 2 decades, the buffalo farming system should be improved to maximize the efficiency of the dairy industry, improve yield and quality of milk and cheese, and work toward better animal welfare.
32229124	3	67	used	used	540:543	arg2	SCC					532:534	SCC	532:534	SCC	532:534	Milk somatic cell count (SCC) is used worldwide as an indicator of udder health in individual milk and is useful for monitoring farm hygiene in bulk milk.
32229124	3	67	used	used	540:543	arg2	count					525:529	Milk somatic cell count	507:529	Milk somatic cell count (SCC)	507:535	Milk somatic cell count (SCC) is used worldwide as an indicator of udder health in individual milk and is useful for monitoring farm hygiene in bulk milk.
32229124	3	67	used	used	540:543	arg2	indicator					561:569	an indicator	558:569	an indicator of udder health	558:585	Milk somatic cell count (SCC) is used worldwide as an indicator of udder health in individual milk and is useful for monitoring farm hygiene in bulk milk.
32229124	3	67	used	used	540:543	arg2	worldwide					545:553	worldwide	545:553	worldwide	545:553	Milk somatic cell count (SCC) is used worldwide as an indicator of udder health in individual milk and is useful for monitoring farm hygiene in bulk milk.
32229124	14	68	with	lactations	2085:2094	arg1	cells/mL					2122:2129	average SCC ≥500,000 cells/mL	2101:2129	average SCC ≥500,000 cells/mL	2101:2129	Supporting this, the proportion of lactations with average SCC ≥500,000 cells/mL increased from 6% in first parity to 12% in sixth parity.
32229124	19	69	theme	specific	2971:2978	arg1	penalties					2980:2988	whenever specific penalties	2962:2988	whenever specific penalties for high SCC	2962:3001	These findings may be helpful for defining reliable and effective SCC thresholds to be adopted whenever specific penalties for high SCC are included in milk payment systems.
32229124	12	70	theme	lactations	1887:1896	arg1	lactations					1887:1896	lactations	1887:1896	lactations	1887:1896	Within first parity, more than 28 and 15% of lactations had average SCC ≥200,000 and ≥300,000 cells/mL, respectively.
32229124	12	70	theme	lactations	1887:1896	arg1	%					1882:1882	more than 28 and 15%	1863:1882	more than 28 and 15% of lactations	1863:1896	Within first parity, more than 28 and 15% of lactations had average SCC ≥200,000 and ≥300,000 cells/mL, respectively.
32229124	13	71	theme	sixth	2022:2026	arg1	parity					2028:2033	sixth parity	2022:2033	sixth parity	2022:2033	Both percentages increased with parity and were 39 and 25% in sixth parity, respectively.
32229124	1	72	theme	PDO	263:265	arg1	cheese					268:273	Origin (PDO) cheese	255:273	Origin (PDO) cheese	255:273	In Southern Italy, buffalo (Bubalus bubalis) milk is mostly intended for the manufacture of Mozzarella di Bufala Campana Protected Denomination of Origin (PDO) cheese.
32229124	9	73	theme	SCC	1400:1402	arg1	characterization					1380:1395	a phenotypic characterization	1367:1395	a phenotypic characterization of SCC	1367:1402	To provide a phenotypic characterization of SCC at the population level and to improve knowledge on buffalo milk quality, 876,299 test-day records of 70,156 buffaloes reared in the PDO area were analyzed.
32229124	12	74	theme	average	1902:1908	arg1	≥200,000					1914:1921	average SCC ≥200,000	1902:1921	average SCC ≥200,000	1902:1921	Within first parity, more than 28 and 15% of lactations had average SCC ≥200,000 and ≥300,000 cells/mL, respectively.
32229124	19	75	theme	payment	3024:3030	arg1	systems					3032:3038	milk payment systems	3019:3038	milk payment systems	3019:3038	These findings may be helpful for defining reliable and effective SCC thresholds to be adopted whenever specific penalties for high SCC are included in milk payment systems.
32229124	1	76	theme	cheese	268:273	arg1	Denomination					239:250	Mozzarella di Bufala Campana Protected Denomination	200:250	Mozzarella di Bufala Campana Protected Denomination of Origin (PDO) cheese	200:273	In Southern Italy, buffalo (Bubalus bubalis) milk is mostly intended for the manufacture of Mozzarella di Bufala Campana Protected Denomination of Origin (PDO) cheese.
32229124	6	77	theme	PDO	978:980	arg1	area					982:985	the PDO area	974:985	the PDO area	974:985	However, payment systems of the PDO area are based simply on the delivered volume of milk rather than on quality characteristics.
32229124	16	78	theme	somatic	2423:2429	arg1	n					2443:2443	n = 6	2443:2447	n = 6	2443:2447	An ANOVA was carried out on test-day record milk yield and composition traits, with fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions; buffalo, herd-test-date, and residual were considered random effects.
32229124	16	78	theme	somatic	2423:2429	arg1	score					2436:2440	somatic cell score	2423:2440	somatic cell score (n = 6)	2423:2448	An ANOVA was carried out on test-day record milk yield and composition traits, with fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions; buffalo, herd-test-date, and residual were considered random effects.
32229124	10	79	theme	SCC	1656:1658	arg1	cells/mL					1669:1676	average milk SCC ≥400,000 cells/mL	1643:1676	average milk SCC ≥400,000 cells/mL	1643:1676	Data revealed that around 11% of herd-test-dates (≥5 animals sampled each) showed average milk SCC ≥400,000 cells/mL (i.e., above the threshold fixed by the European Community for bovine milk).
32229124	20	80	theme	Italian	3191:3197	arg1	population					3207:3216	the Italian buffalo population	3187:3216	the Italian buffalo population	3187:3216	Finally, these results could be used in mastitis monitoring plans aiming to reduce SCC and udder issues at both the individual and farm levels in the Italian buffalo population.
32229124	13	81	from	%	2017:2017	arg1	parity					2028:2033	sixth parity	2022:2033	sixth parity	2022:2033	Both percentages increased with parity and were 39 and 25% in sixth parity, respectively.
32229124	10	82	theme	average	1643:1649	arg1	cells/mL					1669:1676	average milk SCC ≥400,000 cells/mL	1643:1676	average milk SCC ≥400,000 cells/mL	1643:1676	Data revealed that around 11% of herd-test-dates (≥5 animals sampled each) showed average milk SCC ≥400,000 cells/mL (i.e., above the threshold fixed by the European Community for bovine milk).
32229124	0	83	with	relationship	32:43	arg1	quality					65:71	quality	65:71	quality	65:71	Milk somatic cell count and its relationship with milk yield and quality traits in Italian water buffaloes.
32229124	0	83	with	relationship	32:43	arg1	yield					55:59	milk yield	50:59	milk yield	50:59	Milk somatic cell count and its relationship with milk yield and quality traits in Italian water buffaloes.
32229124	6	84	theme	payment	955:961	arg1	systems					963:969	payment systems	955:969	payment systems of the PDO area	955:985	However, payment systems of the PDO area are based simply on the delivered volume of milk rather than on quality characteristics.
32229124	3	85	theme	individual	590:599	arg1	milk					601:604	individual milk	590:604	individual milk	590:604	Milk somatic cell count (SCC) is used worldwide as an indicator of udder health in individual milk and is useful for monitoring farm hygiene in bulk milk.
32229124	3	86	theme	health	580:585	arg1	count					525:529	Milk somatic cell count	507:529	Milk somatic cell count (SCC)	507:535	Milk somatic cell count (SCC) is used worldwide as an indicator of udder health in individual milk and is useful for monitoring farm hygiene in bulk milk.
32229124	3	86	theme	health	580:585	arg1	worldwide					545:553	worldwide	545:553	worldwide	545:553	Milk somatic cell count (SCC) is used worldwide as an indicator of udder health in individual milk and is useful for monitoring farm hygiene in bulk milk.
32229124	3	86	theme	health	580:585	arg1	indicator					561:569	an indicator	558:569	an indicator of udder health	558:585	Milk somatic cell count (SCC) is used worldwide as an indicator of udder health in individual milk and is useful for monitoring farm hygiene in bulk milk.
32229124	1	87	theme	Campana	221:227	arg1	Denomination					239:250	Mozzarella di Bufala Campana Protected Denomination	200:250	Mozzarella di Bufala Campana Protected Denomination of Origin (PDO) cheese	200:273	In Southern Italy, buffalo (Bubalus bubalis) milk is mostly intended for the manufacture of Mozzarella di Bufala Campana Protected Denomination of Origin (PDO) cheese.
32229124	9	88	theme	buffalo	1456:1462	arg1	quality					1469:1475	buffalo milk quality	1456:1475	buffalo milk quality	1456:1475	To provide a phenotypic characterization of SCC at the population level and to improve knowledge on buffalo milk quality, 876,299 test-day records of 70,156 buffaloes reared in the PDO area were analyzed.
32229124	16	89	theme	score	2436:2440	arg1	stage					2407:2411	lactation stage	2397:2411	lactation stage	2397:2411	An ANOVA was carried out on test-day record milk yield and composition traits, with fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions; buffalo, herd-test-date, and residual were considered random effects.
32229124	16	89	theme	score	2436:2440	arg1	interactions					2479:2490	their interactions	2473:2490	their interactions	2473:2490	An ANOVA was carried out on test-day record milk yield and composition traits, with fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions; buffalo, herd-test-date, and residual were considered random effects.
32229124	16	89	theme	score	2436:2440	arg1	month					2451:2455	month	2451:2455	month of calving	2451:2466	An ANOVA was carried out on test-day record milk yield and composition traits, with fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions; buffalo, herd-test-date, and residual were considered random effects.
32229124	16	89	theme	score	2436:2440	arg1	class					2414:2418	class	2414:2418	class of somatic cell score (n = 6)	2414:2448	An ANOVA was carried out on test-day record milk yield and composition traits, with fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions; buffalo, herd-test-date, and residual were considered random effects.
32229124	16	89	theme	score	2436:2440	arg1	parity					2389:2394	parity	2389:2394	parity	2389:2394	An ANOVA was carried out on test-day record milk yield and composition traits, with fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions; buffalo, herd-test-date, and residual were considered random effects.
32229124	20	90	from	levels	3177:3182	arg1	population					3207:3216	the Italian buffalo population	3187:3216	the Italian buffalo population	3187:3216	Finally, these results could be used in mastitis monitoring plans aiming to reduce SCC and udder issues at both the individual and farm levels in the Italian buffalo population.
32229124	1	91	theme	Bubalus	136:142	arg1	milk					153:156	buffalo (Bubalus bubalis) milk	127:156	buffalo (Bubalus bubalis) milk	127:156	In Southern Italy, buffalo (Bubalus bubalis) milk is mostly intended for the manufacture of Mozzarella di Bufala Campana Protected Denomination of Origin (PDO) cheese.
32229124	4	92	theme	large	709:713	arg1	scale					715:719	a large scale	707:719	a large scale in Italy	707:728	Mastitis data are currently not available on a large scale in Italy; thus, SCC is essential for identifying animals with suspected udder infection and inflammation.
32229124	10	93	dep	herd-test-dates	1594:1608	arg1	animals					1614:1620	≥5 animals	1611:1620	≥5 animals sampled each	1611:1633	Data revealed that around 11% of herd-test-dates (≥5 animals sampled each) showed average milk SCC ≥400,000 cells/mL (i.e., above the threshold fixed by the European Community for bovine milk).
32229124	11	94	dep	level	1835:1839	arg1	both					1806:1809	both	1806:1809	both	1806:1809	This suggests that there is room to improve SCC at both the farm and individual level.
32229124	16	95	theme	random	2547:2552	arg1	effects					2554:2560	random effects	2547:2560	random effects	2547:2560	An ANOVA was carried out on test-day record milk yield and composition traits, with fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions; buffalo, herd-test-date, and residual were considered random effects.
32229124	10	96	theme	European	1718:1725	arg1	Community					1727:1735	the European Community	1714:1735	the European Community for bovine milk	1714:1751	Data revealed that around 11% of herd-test-dates (≥5 animals sampled each) showed average milk SCC ≥400,000 cells/mL (i.e., above the threshold fixed by the European Community for bovine milk).
32229124	16	97	theme	calving	2460:2466	arg1	stage					2407:2411	lactation stage	2397:2411	lactation stage	2397:2411	An ANOVA was carried out on test-day record milk yield and composition traits, with fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions; buffalo, herd-test-date, and residual were considered random effects.
32229124	16	97	theme	calving	2460:2466	arg1	interactions					2479:2490	their interactions	2473:2490	their interactions	2473:2490	An ANOVA was carried out on test-day record milk yield and composition traits, with fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions; buffalo, herd-test-date, and residual were considered random effects.
32229124	16	97	theme	calving	2460:2466	arg1	month					2451:2455	month	2451:2455	month of calving	2451:2466	An ANOVA was carried out on test-day record milk yield and composition traits, with fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions; buffalo, herd-test-date, and residual were considered random effects.
32229124	16	97	theme	calving	2460:2466	arg1	class					2414:2418	class	2414:2418	class of somatic cell score (n = 6)	2414:2448	An ANOVA was carried out on test-day record milk yield and composition traits, with fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions; buffalo, herd-test-date, and residual were considered random effects.
32229124	16	97	theme	calving	2460:2466	arg1	parity					2389:2394	parity	2389:2394	parity	2389:2394	An ANOVA was carried out on test-day record milk yield and composition traits, with fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions; buffalo, herd-test-date, and residual were considered random effects.
32229124	19	98	theme	milk	3019:3022	arg1	systems					3032:3038	milk payment systems	3019:3038	milk payment systems	3019:3038	These findings may be helpful for defining reliable and effective SCC thresholds to be adopted whenever specific penalties for high SCC are included in milk payment systems.
32229124	8	99	theme	Community	1259:1267	arg1	regulations					1269:1279	the European Community regulations	1246:1279	the European Community regulations	1246:1279	In addition, SCC for buffalo milk is not mentioned by either the European Community regulations or the PDO protocol, evidencing a lack of rules for the maximum SCC limit.
32229124	20	100	dep	levels	3177:3182	arg1	both					3148:3151	both	3148:3151	both	3148:3151	Finally, these results could be used in mastitis monitoring plans aiming to reduce SCC and udder issues at both the individual and farm levels in the Italian buffalo population.
32229124	7	101	theme	elevated	1120:1127	arg1	SCC					1129:1131	elevated SCC	1120:1131	elevated SCC	1120:1131	Hence, currently there are no penalties for elevated SCC in bulk milk in the Italian buffalo dairy industry.
32229124	3	102	theme	Milk	507:510	arg1	cell					520:523	Milk somatic cell	507:523	Milk somatic cell count (SCC)	507:535	Milk somatic cell count (SCC) is used worldwide as an indicator of udder health in individual milk and is useful for monitoring farm hygiene in bulk milk.
32229124	20	103	theme	monitoring	3090:3099	arg1	plans					3101:3105	mastitis monitoring plans	3081:3105	mastitis monitoring plans aiming to reduce SCC and udder issues at both the individual and farm levels in the Italian buffalo population	3081:3216	Finally, these results could be used in mastitis monitoring plans aiming to reduce SCC and udder issues at both the individual and farm levels in the Italian buffalo population.
32229124	4	104	theme	suspected	783:791	arg1	infection					799:807	suspected udder infection	783:807	suspected udder infection	783:807	Mastitis data are currently not available on a large scale in Italy; thus, SCC is essential for identifying animals with suspected udder infection and inflammation.
32229124	16	105	theme	interactions	2479:2490	arg1	effects					2378:2384	fixed effects	2372:2384	fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions	2372:2490	An ANOVA was carried out on test-day record milk yield and composition traits, with fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions; buffalo, herd-test-date, and residual were considered random effects.
32229124	17	106	dep	estimated	2622:2630	arg1	whereas					2687:2693	whereas	2687:2693	whereas	2687:2693	Significantly lower milk yield and lactose percentage were estimated in progressively higher classes of somatic cell score, whereas no significant differences were observed for fat and protein percentages.
32229124	2	107	theme	better	484:489	arg1	welfare					498:504	better animal welfare	484:504	better animal welfare	484:504	Despite the economic boost of the last 2 decades, the buffalo farming system should be improved to maximize the efficiency of the dairy industry, improve yield and quality of milk and cheese, and work toward better animal welfare.
32229124	8	108	theme	PDO	1288:1290	arg1	protocol					1292:1299	the PDO protocol	1284:1299	the PDO protocol	1284:1299	In addition, SCC for buffalo milk is not mentioned by either the European Community regulations or the PDO protocol, evidencing a lack of rules for the maximum SCC limit.
32229124	6	109	theme	quality	1051:1057	arg1	characteristics					1059:1073	quality characteristics	1051:1073	quality characteristics	1051:1073	However, payment systems of the PDO area are based simply on the delivered volume of milk rather than on quality characteristics.
32229124	3	110	theme	bulk	651:654	arg1	milk					656:659	bulk milk	651:659	bulk milk	651:659	Milk somatic cell count (SCC) is used worldwide as an indicator of udder health in individual milk and is useful for monitoring farm hygiene in bulk milk.
32229124	18	111	theme	milk	2810:2813	arg1	SCC					2815:2817	milk SCC	2810:2817	milk SCC	2810:2817	This is the first attempt to investigate milk SCC in a large data set of Italian dairy buffaloes.
32229124	17	112	theme	milk	2583:2586	arg1	yield					2588:2592	Significantly lower milk yield	2563:2592	Significantly lower milk yield	2563:2592	Significantly lower milk yield and lactose percentage were estimated in progressively higher classes of somatic cell score, whereas no significant differences were observed for fat and protein percentages.
32229124	11	113	theme	farm	1815:1818	arg1	level					1835:1839	both the farm and individual level	1806:1839	level	1835:1839	This suggests that there is room to improve SCC at both the farm and individual level.
32229124	16	114	theme	class	2414:2418	arg1	effects					2378:2384	fixed effects	2372:2384	fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions	2372:2490	An ANOVA was carried out on test-day record milk yield and composition traits, with fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions; buffalo, herd-test-date, and residual were considered random effects.
32229124	5	115	theme	technological	912:924	arg1	properties					926:935	poor technological properties	907:935	poor technological properties of milk	907:943	Moreover, high milk SCC is associated with altered composition and acidity, and poor technological properties of milk.
32229124	3	116	theme	cell	520:523	arg1	count					525:529	Milk somatic cell count	507:529	Milk somatic cell count (SCC)	507:535	Milk somatic cell count (SCC) is used worldwide as an indicator of udder health in individual milk and is useful for monitoring farm hygiene in bulk milk.
32229124	3	116	theme	cell	520:523	arg1	worldwide					545:553	worldwide	545:553	worldwide	545:553	Milk somatic cell count (SCC) is used worldwide as an indicator of udder health in individual milk and is useful for monitoring farm hygiene in bulk milk.
32229124	3	116	theme	cell	520:523	arg1	indicator					561:569	an indicator	558:569	an indicator of udder health	558:585	Milk somatic cell count (SCC) is used worldwide as an indicator of udder health in individual milk and is useful for monitoring farm hygiene in bulk milk.
32229124	3	116	theme	cell	520:523	arg1	SCC					532:534	SCC	532:534	SCC	532:534	Milk somatic cell count (SCC) is used worldwide as an indicator of udder health in individual milk and is useful for monitoring farm hygiene in bulk milk.
32229124	6	117	theme	milk	1031:1034	arg1	volume					1021:1026	the delivered volume	1007:1026	the delivered volume of milk	1007:1034	However, payment systems of the PDO area are based simply on the delivered volume of milk rather than on quality characteristics.
32229124	1	118	theme	buffalo	127:133	arg1	milk					153:156	buffalo (Bubalus bubalis) milk	127:156	buffalo (Bubalus bubalis) milk	127:156	In Southern Italy, buffalo (Bubalus bubalis) milk is mostly intended for the manufacture of Mozzarella di Bufala Campana Protected Denomination of Origin (PDO) cheese.
32229124	2	119	theme	industry	412:419	arg1	efficiency					388:397	the efficiency	384:397	the efficiency of the dairy industry	384:419	Despite the economic boost of the last 2 decades, the buffalo farming system should be improved to maximize the efficiency of the dairy industry, improve yield and quality of milk and cheese, and work toward better animal welfare.
32229124	18	120	theme	large	2824:2828	arg1	set					2835:2837	a large data set	2822:2837	a large data set of Italian dairy buffaloes	2822:2864	This is the first attempt to investigate milk SCC in a large data set of Italian dairy buffaloes.
32229124	18	120	theme	large	2824:2828	arg1	buffaloes					2856:2864	Italian dairy buffaloes	2842:2864	Italian dairy buffaloes	2842:2864	This is the first attempt to investigate milk SCC in a large data set of Italian dairy buffaloes.
32229124	14	121	from	%	2170:2170	arg1	parity					2181:2186	sixth parity	2175:2186	sixth parity	2175:2186	Supporting this, the proportion of lactations with average SCC ≥500,000 cells/mL increased from 6% in first parity to 12% in sixth parity.
32229124	19	122	theme	SCC	2933:2935	arg1	thresholds					2937:2946	reliable and effective SCC thresholds	2910:2946	reliable and effective SCC thresholds	2910:2946	These findings may be helpful for defining reliable and effective SCC thresholds to be adopted whenever specific penalties for high SCC are included in milk payment systems.
32229124	0	123	theme	water	91:95	arg1	buffaloes					97:105	Italian water buffaloes	83:105	Italian water buffaloes	83:105	Milk somatic cell count and its relationship with milk yield and quality traits in Italian water buffaloes.
32229124	5	124	theme	high	837:840	arg1	SCC					847:849	high milk SCC	837:849	high milk SCC	837:849	Moreover, high milk SCC is associated with altered composition and acidity, and poor technological properties of milk.
32229124	7	125	theme	Italian	1153:1159	arg1	industry					1175:1182	the Italian buffalo dairy industry	1149:1182	the Italian buffalo dairy industry	1149:1182	Hence, currently there are no penalties for elevated SCC in bulk milk in the Italian buffalo dairy industry.
32229124	8	126	theme	maximum	1337:1343	arg1	limit					1349:1353	the maximum SCC limit	1333:1353	the maximum SCC limit	1333:1353	In addition, SCC for buffalo milk is not mentioned by either the European Community regulations or the PDO protocol, evidencing a lack of rules for the maximum SCC limit.
32229124	0	127	theme	somatic	5:11	arg1	count					18:22	Milk somatic cell count	0:22	Milk somatic cell count	0:22	Milk somatic cell count and its relationship with milk yield and quality traits in Italian water buffaloes.
32229124	15	128	theme	Milk	2189:2192	arg1	yield					2194:2198	Milk yield	2189:2198	Milk yield	2189:2198	Milk yield and SCC were negatively correlated with each other, especially when SCC level was high.
32229124	18	129	theme	Italian	2842:2848	arg1	buffaloes					2856:2864	Italian dairy buffaloes	2842:2864	Italian dairy buffaloes	2842:2864	This is the first attempt to investigate milk SCC in a large data set of Italian dairy buffaloes.
32229124	4	130	with	animals	770:776	arg1	inflammation					813:824	inflammation	813:824	inflammation	813:824	Mastitis data are currently not available on a large scale in Italy; thus, SCC is essential for identifying animals with suspected udder infection and inflammation.
32229124	4	130	with	animals	770:776	arg1	infection					799:807	suspected udder infection	783:807	suspected udder infection	783:807	Mastitis data are currently not available on a large scale in Italy; thus, SCC is essential for identifying animals with suspected udder infection and inflammation.
32229124	2	131	theme	cheese	460:465	arg1	quality					440:446	quality	440:446	quality	440:446	Despite the economic boost of the last 2 decades, the buffalo farming system should be improved to maximize the efficiency of the dairy industry, improve yield and quality of milk and cheese, and work toward better animal welfare.
32229124	2	131	theme	cheese	460:465	arg1	yield					430:434	yield	430:434	yield	430:434	Despite the economic boost of the last 2 decades, the buffalo farming system should be improved to maximize the efficiency of the dairy industry, improve yield and quality of milk and cheese, and work toward better animal welfare.
32229124	3	132	from	hygiene	640:646	arg1	milk					656:659	bulk milk	651:659	bulk milk	651:659	Milk somatic cell count (SCC) is used worldwide as an indicator of udder health in individual milk and is useful for monitoring farm hygiene in bulk milk.
32229124	12	133	theme	SCC	1910:1912	arg1	≥200,000					1914:1921	average SCC ≥200,000	1902:1921	average SCC ≥200,000	1902:1921	Within first parity, more than 28 and 15% of lactations had average SCC ≥200,000 and ≥300,000 cells/mL, respectively.
32229124	2	134	theme	decades	317:323	arg1	economic					288:295	economic	288:295	economic	288:295	Despite the economic boost of the last 2 decades, the buffalo farming system should be improved to maximize the efficiency of the dairy industry, improve yield and quality of milk and cheese, and work toward better animal welfare.
32229124	9	135	theme	phenotypic	1369:1378	arg1	characterization					1380:1395	a phenotypic characterization	1367:1395	a phenotypic characterization of SCC	1367:1402	To provide a phenotypic characterization of SCC at the population level and to improve knowledge on buffalo milk quality, 876,299 test-day records of 70,156 buffaloes reared in the PDO area were analyzed.
32229124	7	136	theme	bulk	1136:1139	arg1	milk					1141:1144	bulk milk	1136:1144	bulk milk in the Italian buffalo dairy industry	1136:1182	Hence, currently there are no penalties for elevated SCC in bulk milk in the Italian buffalo dairy industry.
32229124	14	137	theme	SCC	2109:2111	arg1	cells/mL					2122:2129	average SCC ≥500,000 cells/mL	2101:2129	average SCC ≥500,000 cells/mL	2101:2129	Supporting this, the proportion of lactations with average SCC ≥500,000 cells/mL increased from 6% in first parity to 12% in sixth parity.
32229124	16	138	theme	cell	2431:2434	arg1	n					2443:2443	n = 6	2443:2447	n = 6	2443:2447	An ANOVA was carried out on test-day record milk yield and composition traits, with fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions; buffalo, herd-test-date, and residual were considered random effects.
32229124	16	138	theme	cell	2431:2434	arg1	score					2436:2440	somatic cell score	2423:2440	somatic cell score (n = 6)	2423:2448	An ANOVA was carried out on test-day record milk yield and composition traits, with fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions; buffalo, herd-test-date, and residual were considered random effects.
32229124	19	139	theme	whenever	2962:2969	arg1	penalties					2980:2988	whenever specific penalties	2962:2988	whenever specific penalties for high SCC	2962:3001	These findings may be helpful for defining reliable and effective SCC thresholds to be adopted whenever specific penalties for high SCC are included in milk payment systems.
32229124	2	140	dep	economic	288:295	arg1	boost					297:301	boost	297:301	boost	297:301	Despite the economic boost of the last 2 decades, the buffalo farming system should be improved to maximize the efficiency of the dairy industry, improve yield and quality of milk and cheese, and work toward better animal welfare.
32229124	5	141	theme	altered	870:876	arg1	composition					878:888	altered composition	870:888	altered composition	870:888	Moreover, high milk SCC is associated with altered composition and acidity, and poor technological properties of milk.
32229124	16	142	theme	parity	2389:2394	arg1	effects					2378:2384	fixed effects	2372:2384	fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions	2372:2490	An ANOVA was carried out on test-day record milk yield and composition traits, with fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions; buffalo, herd-test-date, and residual were considered random effects.
32229124	8	143	theme	buffalo	1206:1212	arg1	milk					1214:1217	buffalo milk	1206:1217	buffalo milk	1206:1217	In addition, SCC for buffalo milk is not mentioned by either the European Community regulations or the PDO protocol, evidencing a lack of rules for the maximum SCC limit.
32229124	0	144	with	count	18:22	arg1	quality					65:71	quality	65:71	quality	65:71	Milk somatic cell count and its relationship with milk yield and quality traits in Italian water buffaloes.
32229124	0	144	with	count	18:22	arg1	yield					55:59	milk yield	50:59	milk yield	50:59	Milk somatic cell count and its relationship with milk yield and quality traits in Italian water buffaloes.
32229124	2	145	theme	farming	338:344	arg1	system					346:351	the buffalo farming system	326:351	the buffalo farming system	326:351	Despite the economic boost of the last 2 decades, the buffalo farming system should be improved to maximize the efficiency of the dairy industry, improve yield and quality of milk and cheese, and work toward better animal welfare.
32229124	6	146	theme	area	982:985	arg1	systems					963:969	payment systems	955:969	payment systems of the PDO area	955:985	However, payment systems of the PDO area are based simply on the delivered volume of milk rather than on quality characteristics.
32229124	18	147	theme	dairy	2850:2854	arg1	buffaloes					2856:2864	Italian dairy buffaloes	2842:2864	Italian dairy buffaloes	2842:2864	This is the first attempt to investigate milk SCC in a large data set of Italian dairy buffaloes.
32229124	19	148	theme	high	2994:2997	arg1	SCC					2999:3001	high SCC	2994:3001	high SCC	2994:3001	These findings may be helpful for defining reliable and effective SCC thresholds to be adopted whenever specific penalties for high SCC are included in milk payment systems.
32229124	20	149	theme	buffalo	3199:3205	arg1	population					3207:3216	the Italian buffalo population	3187:3216	the Italian buffalo population	3187:3216	Finally, these results could be used in mastitis monitoring plans aiming to reduce SCC and udder issues at both the individual and farm levels in the Italian buffalo population.
32229124	9	150	theme	population	1411:1420	arg1	level					1422:1426	the population level	1407:1426	the population level	1407:1426	To provide a phenotypic characterization of SCC at the population level and to improve knowledge on buffalo milk quality, 876,299 test-day records of 70,156 buffaloes reared in the PDO area were analyzed.
32229124	7	151	from	milk	1141:1144	arg1	industry					1175:1182	the Italian buffalo dairy industry	1149:1182	the Italian buffalo dairy industry	1149:1182	Hence, currently there are no penalties for elevated SCC in bulk milk in the Italian buffalo dairy industry.
32229124	16	152	theme	test-day	2316:2323	arg1	yield					2337:2341	test-day record milk yield	2316:2341	test-day record milk yield	2316:2341	An ANOVA was carried out on test-day record milk yield and composition traits, with fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions; buffalo, herd-test-date, and residual were considered random effects.
32229124	10	153	theme	milk	1651:1654	arg1	cells/mL					1669:1676	average milk SCC ≥400,000 cells/mL	1643:1676	average milk SCC ≥400,000 cells/mL	1643:1676	Data revealed that around 11% of herd-test-dates (≥5 animals sampled each) showed average milk SCC ≥400,000 cells/mL (i.e., above the threshold fixed by the European Community for bovine milk).
32229124	4	154	theme	Mastitis	662:669	arg1	data					671:674	Mastitis data	662:674	Mastitis data	662:674	Mastitis data are currently not available on a large scale in Italy; thus, SCC is essential for identifying animals with suspected udder infection and inflammation.
32229124	16	155	theme	record	2325:2330	arg1	yield					2337:2341	test-day record milk yield	2316:2341	test-day record milk yield	2316:2341	An ANOVA was carried out on test-day record milk yield and composition traits, with fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions; buffalo, herd-test-date, and residual were considered random effects.
32229124	17	156	dep	fat	2740:2742	arg1	percentages					2756:2766	percentages	2756:2766	percentages	2756:2766	Significantly lower milk yield and lactose percentage were estimated in progressively higher classes of somatic cell score, whereas no significant differences were observed for fat and protein percentages.
32229124	14	157	theme	sixth	2175:2179	arg1	parity					2181:2186	sixth parity	2175:2186	sixth parity	2175:2186	Supporting this, the proportion of lactations with average SCC ≥500,000 cells/mL increased from 6% in first parity to 12% in sixth parity.
32229124	16	158	theme	milk	2332:2335	arg1	yield					2337:2341	test-day record milk yield	2316:2341	test-day record milk yield	2316:2341	An ANOVA was carried out on test-day record milk yield and composition traits, with fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions; buffalo, herd-test-date, and residual were considered random effects.
32229124	16	159	theme	composition	2347:2357	arg1	traits					2359:2364	composition traits	2347:2364	composition traits	2347:2364	An ANOVA was carried out on test-day record milk yield and composition traits, with fixed effects of parity, lactation stage, class of somatic cell score (n = 6), month of calving, and their interactions; buffalo, herd-test-date, and residual were considered random effects.
32229124	17	160	theme	somatic	2667:2673	arg1	score					2680:2684	somatic cell score	2667:2684	somatic cell score	2667:2684	Significantly lower milk yield and lactose percentage were estimated in progressively higher classes of somatic cell score, whereas no significant differences were observed for fat and protein percentages.
32229124	14	161	theme	average	2101:2107	arg1	cells/mL					2122:2129	average SCC ≥500,000 cells/mL	2101:2129	average SCC ≥500,000 cells/mL	2101:2129	Supporting this, the proportion of lactations with average SCC ≥500,000 cells/mL increased from 6% in first parity to 12% in sixth parity.
32229124	2	162	theme	last	310:313	arg1	decades					317:323	the last 2 decades	306:323	the last 2 decades	306:323	Despite the economic boost of the last 2 decades, the buffalo farming system should be improved to maximize the efficiency of the dairy industry, improve yield and quality of milk and cheese, and work toward better animal welfare.
33276449	8	0	theme	intricate	1395:1403	arg1	pathways					1415:1422	intricate metabolic pathways	1395:1422	intricate metabolic pathways	1395:1422	The candidates of this study are likely involved in intricate metabolic pathways and represent candidate gene targets for source-sink activities and drought and heat stress tolerance during grain filling.
33276449	1	1	theme	grains	148:153	arg1	endosperm					128:136	the endosperm	124:136	the endosperm of cereal grains	124:153	Starch accumulated in the endosperm of cereal grains as reserve energy for germination serves as a staple in human and animal nutrition.
33276449	6	2	theme	biochemical	1198:1208	arg1	pathways					1210:1217	five biochemical pathways	1193:1217	five biochemical pathways including protein processing	1193:1246	The HSP90 is a potential hub gene with gene network of 75 high-confidence first interactors that is enriched for five biochemical pathways including protein processing.
33276449	6	2	theme	biochemical	1198:1208	arg1	processing					1237:1246	protein processing	1229:1246	protein processing	1229:1246	The HSP90 is a potential hub gene with gene network of 75 high-confidence first interactors that is enriched for five biochemical pathways including protein processing.
33276449	0	3	theme	Starch	75:80	arg1	Content					82:88	Grain Starch Content	69:88	Grain Starch Content in Sorghum	69:99	Identification of Novel Genomic Associations and Gene Candidates for Grain Starch Content in Sorghum.
33276449	5	4	theme	reproductive	1016:1027	arg1	tissues					1029:1035	reproductive tissues	1016:1035	reproductive tissues	1016:1035	All significant SNPs were located within protein coding genes, with SNPs ∼ 52 Mb of chr-8 encoding a Casperian strip membrane protein (CASP)-like protein (Sobic.008G111500) and a heat shock protein (HSP) 90 (Sobic.008G111600) that were highly expressed in reproductive tissues including within the embryo and endosperm.
33276449	3	5	theme	linear	477:482	arg1	LMM					498:500	LMM	498:500	LMM	498:500	In this study, we used a sorghum association panel with 389 individuals and 141,557 single nucleotide polymorphisms (SNPs) to fit linear mixed models (LMM) for identifying genomic regions and potential candidate genes associated with starch content.
33276449	3	5	theme	linear	477:482	arg1	models					490:495	linear mixed models	477:495	linear mixed models (LMM) for identifying genomic regions and potential candidate genes associated with starch content	477:594	In this study, we used a sorghum association panel with 389 individuals and 141,557 single nucleotide polymorphisms (SNPs) to fit linear mixed models (LMM) for identifying genomic regions and potential candidate genes associated with starch content.
33276449	4	6	theme	LMM	731:733	arg1	combination					716:726	combination	716:726	combination of LMM and Bayesian sparse LMM	716:757	Three associated genomic regions, one in chromosome (chr) 1 and two novel associations in chr-8, were identified using combination of LMM and Bayesian sparse LMM.
33276449	6	7	theme	interactors	1160:1170	arg1	network					1124:1130	gene network	1119:1130	gene network of 75 high-confidence first interactors that is enriched for five biochemical pathways including protein processing	1119:1246	The HSP90 is a potential hub gene with gene network of 75 high-confidence first interactors that is enriched for five biochemical pathways including protein processing.
33276449	6	8	theme	potential	1095:1103	arg1	gene					1109:1112	a potential hub gene	1093:1112	a potential hub gene with gene network of 75 high-confidence first interactors that is enriched for five biochemical pathways including protein processing	1093:1246	The HSP90 is a potential hub gene with gene network of 75 high-confidence first interactors that is enriched for five biochemical pathways including protein processing.
33276449	6	8	theme	potential	1095:1103	arg1	HSP90					1084:1088	The HSP90	1080:1088	The HSP90	1080:1088	The HSP90 is a potential hub gene with gene network of 75 high-confidence first interactors that is enriched for five biochemical pathways including protein processing.
33276449	7	9	theme	transcript	1297:1306	arg1	abundance					1308:1316	high transcript abundance	1292:1316	high transcript abundance in reproductive tissues	1292:1340	The first interactors of HSP90 also showed high transcript abundance in reproductive tissues.
33276449	8	10	theme	candidate	1438:1446	arg1	targets					1453:1459	candidate gene targets	1438:1459	candidate gene targets for source-sink activities and drought and heat stress tolerance during grain filling	1438:1545	The candidates of this study are likely involved in intricate metabolic pathways and represent candidate gene targets for source-sink activities and drought and heat stress tolerance during grain filling.
33276449	6	11	theme	first	1154:1158	arg1	interactors					1160:1170	75 high-confidence first interactors	1135:1170	75 high-confidence first interactors that is enriched for five biochemical pathways including protein processing	1135:1246	The HSP90 is a potential hub gene with gene network of 75 high-confidence first interactors that is enriched for five biochemical pathways including protein processing.
33276449	2	12	theme	starch	270:275	arg1	metabolism					277:286	starch metabolism	270:286	starch metabolism	270:286	Unraveling genetic control for starch metabolism is important for breeding grains with high starch content.
33276449	5	13	theme	protein	801:807	arg1	genes					816:820	protein coding genes	801:820	protein coding genes	801:820	All significant SNPs were located within protein coding genes, with SNPs ∼ 52 Mb of chr-8 encoding a Casperian strip membrane protein (CASP)-like protein (Sobic.008G111500) and a heat shock protein (HSP) 90 (Sobic.008G111600) that were highly expressed in reproductive tissues including within the embryo and endosperm.
33276449	6	14	theme	high-confidence	1138:1152	arg1	interactors					1160:1170	75 high-confidence first interactors	1135:1170	75 high-confidence first interactors that is enriched for five biochemical pathways including protein processing	1135:1246	The HSP90 is a potential hub gene with gene network of 75 high-confidence first interactors that is enriched for five biochemical pathways including protein processing.
33276449	7	15	theme	reproductive	1321:1332	arg1	tissues					1334:1340	reproductive tissues	1321:1340	reproductive tissues	1321:1340	The first interactors of HSP90 also showed high transcript abundance in reproductive tissues.
33276449	1	16	theme	reserve	158:164	arg1	energy					166:171	reserve energy	158:171	reserve energy for germination	158:187	Starch accumulated in the endosperm of cereal grains as reserve energy for germination serves as a staple in human and animal nutrition.
33276449	1	16	theme	reserve	158:164	arg1	staple					201:206	staple	201:206	staple	201:206	Starch accumulated in the endosperm of cereal grains as reserve energy for germination serves as a staple in human and animal nutrition.
33276449	3	17	theme	sorghum	372:378	arg1	panel					392:396	a sorghum association panel	370:396	a sorghum association panel with 389 individuals and 141,557 single nucleotide polymorphisms (SNPs)	370:468	In this study, we used a sorghum association panel with 389 individuals and 141,557 single nucleotide polymorphisms (SNPs) to fit linear mixed models (LMM) for identifying genomic regions and potential candidate genes associated with starch content.
33276449	3	18	with	panel	392:396	arg1	individuals					407:417	389 individuals	403:417	389 individuals	403:417	In this study, we used a sorghum association panel with 389 individuals and 141,557 single nucleotide polymorphisms (SNPs) to fit linear mixed models (LMM) for identifying genomic regions and potential candidate genes associated with starch content.
33276449	3	18	with	panel	392:396	arg1	SNPs					464:467	SNPs	464:467	SNPs	464:467	In this study, we used a sorghum association panel with 389 individuals and 141,557 single nucleotide polymorphisms (SNPs) to fit linear mixed models (LMM) for identifying genomic regions and potential candidate genes associated with starch content.
33276449	3	18	with	panel	392:396	arg1	polymorphisms					449:461	141,557 single nucleotide polymorphisms	423:461	141,557 single nucleotide polymorphisms (SNPs)	423:468	In this study, we used a sorghum association panel with 389 individuals and 141,557 single nucleotide polymorphisms (SNPs) to fit linear mixed models (LMM) for identifying genomic regions and potential candidate genes associated with starch content.
33276449	4	19	from	one	631:633	arg1	chr-8					687:691	chr-8	687:691	chr-8	687:691	Three associated genomic regions, one in chromosome (chr) 1 and two novel associations in chr-8, were identified using combination of LMM and Bayesian sparse LMM.
33276449	4	19	from	one	631:633	arg1	chr					650:652	chromosome (chr) 1	638:655	chromosome (chr) 1	638:655	Three associated genomic regions, one in chromosome (chr) 1 and two novel associations in chr-8, were identified using combination of LMM and Bayesian sparse LMM.
33276449	5	20	theme	coding	809:814	arg1	genes					816:820	protein coding genes	801:820	protein coding genes	801:820	All significant SNPs were located within protein coding genes, with SNPs ∼ 52 Mb of chr-8 encoding a Casperian strip membrane protein (CASP)-like protein (Sobic.008G111500) and a heat shock protein (HSP) 90 (Sobic.008G111600) that were highly expressed in reproductive tissues including within the embryo and endosperm.
33276449	8	21	theme	gene	1448:1451	arg1	targets					1453:1459	candidate gene targets	1438:1459	candidate gene targets for source-sink activities and drought and heat stress tolerance during grain filling	1438:1545	The candidates of this study are likely involved in intricate metabolic pathways and represent candidate gene targets for source-sink activities and drought and heat stress tolerance during grain filling.
33276449	2	22	theme	breeding	305:312	arg1	grains					314:319	breeding grains	305:319	breeding grains with high starch content	305:344	Unraveling genetic control for starch metabolism is important for breeding grains with high starch content.
33276449	4	23	theme	chromosome	638:647	arg1	chr					650:652	chromosome (chr) 1	638:655	chromosome (chr) 1	638:655	Three associated genomic regions, one in chromosome (chr) 1 and two novel associations in chr-8, were identified using combination of LMM and Bayesian sparse LMM.
33276449	8	24	theme	source-sink	1465:1475	arg1	activities					1477:1486	source-sink activities	1465:1486	source-sink activities	1465:1486	The candidates of this study are likely involved in intricate metabolic pathways and represent candidate gene targets for source-sink activities and drought and heat stress tolerance during grain filling.
33276449	5	25	located	located	786:792	arg2	SNPs					776:779	All significant SNPs	760:779	All significant SNPs	760:779	All significant SNPs were located within protein coding genes, with SNPs ∼ 52 Mb of chr-8 encoding a Casperian strip membrane protein (CASP)-like protein (Sobic.008G111500) and a heat shock protein (HSP) 90 (Sobic.008G111600) that were highly expressed in reproductive tissues including within the embryo and endosperm.
33276449	5	25	located	located	786:792	arg1	genes					816:820	protein coding genes	801:820	protein coding genes	801:820	All significant SNPs were located within protein coding genes, with SNPs ∼ 52 Mb of chr-8 encoding a Casperian strip membrane protein (CASP)-like protein (Sobic.008G111500) and a heat shock protein (HSP) 90 (Sobic.008G111600) that were highly expressed in reproductive tissues including within the embryo and endosperm.
33276449	7	26	theme	first	1253:1257	arg1	interactors					1259:1269	The first interactors	1249:1269	The first interactors of HSP90	1249:1278	The first interactors of HSP90 also showed high transcript abundance in reproductive tissues.
33276449	3	27	theme	389	403:405	arg1	individuals					407:417	389 individuals	403:417	389 individuals	403:417	In this study, we used a sorghum association panel with 389 individuals and 141,557 single nucleotide polymorphisms (SNPs) to fit linear mixed models (LMM) for identifying genomic regions and potential candidate genes associated with starch content.
33276449	0	28	theme	Genomic	24:30	arg1	Associations					32:43	Novel Genomic Associations	18:43	Novel Genomic Associations	18:43	Identification of Novel Genomic Associations and Gene Candidates for Grain Starch Content in Sorghum.
33276449	4	29	dep	one	631:633	arg1	associations					671:682	novel associations	665:682	novel associations	665:682	Three associated genomic regions, one in chromosome (chr) 1 and two novel associations in chr-8, were identified using combination of LMM and Bayesian sparse LMM.
33276449	5	30	with	located	786:792	arg1	SNPs					828:831	SNPs	828:831	SNPs ∼ 52 Mb of chr-8 encoding a Casperian strip membrane protein (CASP)-like protein (Sobic.008G111500) and a heat shock protein (HSP) 90 (Sobic.008G111600) that were highly expressed in reproductive tissues including within the embryo and endosperm	828:1077	All significant SNPs were located within protein coding genes, with SNPs ∼ 52 Mb of chr-8 encoding a Casperian strip membrane protein (CASP)-like protein (Sobic.008G111500) and a heat shock protein (HSP) 90 (Sobic.008G111600) that were highly expressed in reproductive tissues including within the embryo and endosperm.
33276449	5	31	theme	heat	939:942	arg1	HSP					959:961	HSP	959:961	HSP	959:961	All significant SNPs were located within protein coding genes, with SNPs ∼ 52 Mb of chr-8 encoding a Casperian strip membrane protein (CASP)-like protein (Sobic.008G111500) and a heat shock protein (HSP) 90 (Sobic.008G111600) that were highly expressed in reproductive tissues including within the embryo and endosperm.
33276449	5	31	theme	heat	939:942	arg1	protein					950:956	a heat shock protein (HSP) 90	937:965	a heat shock protein (HSP) 90	937:965	All significant SNPs were located within protein coding genes, with SNPs ∼ 52 Mb of chr-8 encoding a Casperian strip membrane protein (CASP)-like protein (Sobic.008G111500) and a heat shock protein (HSP) 90 (Sobic.008G111600) that were highly expressed in reproductive tissues including within the embryo and endosperm.
33276449	7	32	theme	high	1292:1295	arg1	abundance					1308:1316	high transcript abundance	1292:1316	high transcript abundance in reproductive tissues	1292:1340	The first interactors of HSP90 also showed high transcript abundance in reproductive tissues.
33276449	3	33	theme	nucleotide	438:447	arg1	SNPs					464:467	SNPs	464:467	SNPs	464:467	In this study, we used a sorghum association panel with 389 individuals and 141,557 single nucleotide polymorphisms (SNPs) to fit linear mixed models (LMM) for identifying genomic regions and potential candidate genes associated with starch content.
33276449	3	33	theme	nucleotide	438:447	arg1	polymorphisms					449:461	141,557 single nucleotide polymorphisms	423:461	141,557 single nucleotide polymorphisms (SNPs)	423:468	In this study, we used a sorghum association panel with 389 individuals and 141,557 single nucleotide polymorphisms (SNPs) to fit linear mixed models (LMM) for identifying genomic regions and potential candidate genes associated with starch content.
33276449	0	34	theme	Novel	18:22	arg1	Associations					32:43	Novel Genomic Associations	18:43	Novel Genomic Associations	18:43	Identification of Novel Genomic Associations and Gene Candidates for Grain Starch Content in Sorghum.
33276449	6	35	theme	protein	1229:1235	arg1	processing					1237:1246	protein processing	1229:1246	protein processing	1229:1246	The HSP90 is a potential hub gene with gene network of 75 high-confidence first interactors that is enriched for five biochemical pathways including protein processing.
33276449	0	36	from	Content	82:88	arg1	Sorghum					93:99	Sorghum	93:99	Sorghum	93:99	Identification of Novel Genomic Associations and Gene Candidates for Grain Starch Content in Sorghum.
33276449	3	37	theme	genomic	519:525	arg1	regions					527:533	genomic regions	519:533	genomic regions	519:533	In this study, we used a sorghum association panel with 389 individuals and 141,557 single nucleotide polymorphisms (SNPs) to fit linear mixed models (LMM) for identifying genomic regions and potential candidate genes associated with starch content.
33276449	3	38	theme	association	380:390	arg1	panel					392:396	a sorghum association panel	370:396	a sorghum association panel with 389 individuals and 141,557 single nucleotide polymorphisms (SNPs)	370:468	In this study, we used a sorghum association panel with 389 individuals and 141,557 single nucleotide polymorphisms (SNPs) to fit linear mixed models (LMM) for identifying genomic regions and potential candidate genes associated with starch content.
33276449	8	39	theme	heat	1504:1507	arg1	tolerance					1516:1524	heat stress tolerance	1504:1524	heat stress tolerance	1504:1524	The candidates of this study are likely involved in intricate metabolic pathways and represent candidate gene targets for source-sink activities and drought and heat stress tolerance during grain filling.
33276449	3	40	used	used	365:368	arg2	we					362:363	we	362:363	we	362:363	In this study, we used a sorghum association panel with 389 individuals and 141,557 single nucleotide polymorphisms (SNPs) to fit linear mixed models (LMM) for identifying genomic regions and potential candidate genes associated with starch content.
33276449	2	41	with	grains	314:319	arg1	content					338:344	high starch content	326:344	high starch content	326:344	Unraveling genetic control for starch metabolism is important for breeding grains with high starch content.
33276449	6	42	theme	gene	1119:1122	arg1	network					1124:1130	gene network	1119:1130	gene network of 75 high-confidence first interactors that is enriched for five biochemical pathways including protein processing	1119:1246	The HSP90 is a potential hub gene with gene network of 75 high-confidence first interactors that is enriched for five biochemical pathways including protein processing.
33276449	0	43	theme	Associations	32:43	arg1	Identification					0:13	Identification	0:13	Identification of Novel Genomic Associations and Gene Candidates for Grain Starch Content in Sorghum.	0:100	Identification of Novel Genomic Associations and Gene Candidates for Grain Starch Content in Sorghum.
33276449	8	44	theme	study	1366:1370	arg1	candidates					1347:1356	The candidates	1343:1356	The candidates of this study	1343:1370	The candidates of this study are likely involved in intricate metabolic pathways and represent candidate gene targets for source-sink activities and drought and heat stress tolerance during grain filling.
33276449	4	45	theme	LMM	755:757	arg1	combination					716:726	combination	716:726	combination of LMM and Bayesian sparse LMM	716:757	Three associated genomic regions, one in chromosome (chr) 1 and two novel associations in chr-8, were identified using combination of LMM and Bayesian sparse LMM.
33276449	7	46	theme	HSP90	1274:1278	arg1	interactors					1259:1269	The first interactors	1249:1269	The first interactors of HSP90	1249:1278	The first interactors of HSP90 also showed high transcript abundance in reproductive tissues.
33276449	3	47	theme	single	431:436	arg1	SNPs					464:467	SNPs	464:467	SNPs	464:467	In this study, we used a sorghum association panel with 389 individuals and 141,557 single nucleotide polymorphisms (SNPs) to fit linear mixed models (LMM) for identifying genomic regions and potential candidate genes associated with starch content.
33276449	3	47	theme	single	431:436	arg1	polymorphisms					449:461	141,557 single nucleotide polymorphisms	423:461	141,557 single nucleotide polymorphisms (SNPs)	423:468	In this study, we used a sorghum association panel with 389 individuals and 141,557 single nucleotide polymorphisms (SNPs) to fit linear mixed models (LMM) for identifying genomic regions and potential candidate genes associated with starch content.
33276449	0	48	theme	Candidates	54:63	arg1	Identification					0:13	Identification	0:13	Identification of Novel Genomic Associations and Gene Candidates for Grain Starch Content in Sorghum.	0:100	Identification of Novel Genomic Associations and Gene Candidates for Grain Starch Content in Sorghum.
33276449	5	49	theme	significant	764:774	arg1	SNPs					776:779	All significant SNPs	760:779	All significant SNPs	760:779	All significant SNPs were located within protein coding genes, with SNPs ∼ 52 Mb of chr-8 encoding a Casperian strip membrane protein (CASP)-like protein (Sobic.008G111500) and a heat shock protein (HSP) 90 (Sobic.008G111600) that were highly expressed in reproductive tissues including within the embryo and endosperm.
33276449	1	50	dep	human	211:215	arg1	nutrition					228:236	nutrition	228:236	nutrition	228:236	Starch accumulated in the endosperm of cereal grains as reserve energy for germination serves as a staple in human and animal nutrition.
33276449	3	51	theme	potential	539:547	arg1	genes					559:563	potential candidate genes	539:563	potential candidate genes	539:563	In this study, we used a sorghum association panel with 389 individuals and 141,557 single nucleotide polymorphisms (SNPs) to fit linear mixed models (LMM) for identifying genomic regions and potential candidate genes associated with starch content.
33276449	1	52	from	staple	201:206	arg1	animal					221:226	animal	221:226	animal	221:226	Starch accumulated in the endosperm of cereal grains as reserve energy for germination serves as a staple in human and animal nutrition.
33276449	1	52	from	staple	201:206	arg1	human					211:215	human	211:215	human	211:215	Starch accumulated in the endosperm of cereal grains as reserve energy for germination serves as a staple in human and animal nutrition.
33276449	2	53	theme	starch	331:336	arg1	content					338:344	high starch content	326:344	high starch content	326:344	Unraveling genetic control for starch metabolism is important for breeding grains with high starch content.
33276449	0	54	theme	Gene	49:52	arg1	Candidates					54:63	Gene Candidates	49:63	Gene Candidates for Grain Starch Content in Sorghum	49:99	Identification of Novel Genomic Associations and Gene Candidates for Grain Starch Content in Sorghum.
33276449	8	55	theme	stress	1509:1514	arg1	tolerance					1516:1524	heat stress tolerance	1504:1524	heat stress tolerance	1504:1524	The candidates of this study are likely involved in intricate metabolic pathways and represent candidate gene targets for source-sink activities and drought and heat stress tolerance during grain filling.
33276449	1	56	from	animal	221:226	arg1	energy					166:171	reserve energy	158:171	reserve energy for germination	158:187	Starch accumulated in the endosperm of cereal grains as reserve energy for germination serves as a staple in human and animal nutrition.
33276449	1	56	from	animal	221:226	arg1	staple					201:206	staple	201:206	staple	201:206	Starch accumulated in the endosperm of cereal grains as reserve energy for germination serves as a staple in human and animal nutrition.
33276449	5	57	theme	shock	944:948	arg1	HSP					959:961	HSP	959:961	HSP	959:961	All significant SNPs were located within protein coding genes, with SNPs ∼ 52 Mb of chr-8 encoding a Casperian strip membrane protein (CASP)-like protein (Sobic.008G111500) and a heat shock protein (HSP) 90 (Sobic.008G111600) that were highly expressed in reproductive tissues including within the embryo and endosperm.
33276449	5	57	theme	shock	944:948	arg1	protein					950:956	a heat shock protein (HSP) 90	937:965	a heat shock protein (HSP) 90	937:965	All significant SNPs were located within protein coding genes, with SNPs ∼ 52 Mb of chr-8 encoding a Casperian strip membrane protein (CASP)-like protein (Sobic.008G111500) and a heat shock protein (HSP) 90 (Sobic.008G111600) that were highly expressed in reproductive tissues including within the embryo and endosperm.
33276449	3	58	theme	candidate	549:557	arg1	genes					559:563	potential candidate genes	539:563	potential candidate genes	539:563	In this study, we used a sorghum association panel with 389 individuals and 141,557 single nucleotide polymorphisms (SNPs) to fit linear mixed models (LMM) for identifying genomic regions and potential candidate genes associated with starch content.
33276449	2	59	theme	high	326:329	arg1	content					338:344	high starch content	326:344	high starch content	326:344	Unraveling genetic control for starch metabolism is important for breeding grains with high starch content.
33276449	6	60	theme	hub	1105:1107	arg1	gene					1109:1112	a potential hub gene	1093:1112	a potential hub gene with gene network of 75 high-confidence first interactors that is enriched for five biochemical pathways including protein processing	1093:1246	The HSP90 is a potential hub gene with gene network of 75 high-confidence first interactors that is enriched for five biochemical pathways including protein processing.
33276449	6	60	theme	hub	1105:1107	arg1	HSP90					1084:1088	The HSP90	1080:1088	The HSP90	1080:1088	The HSP90 is a potential hub gene with gene network of 75 high-confidence first interactors that is enriched for five biochemical pathways including protein processing.
33276449	4	61	theme	Bayesian	739:746	arg1	LMM					755:757	Bayesian sparse LMM	739:757	Bayesian sparse LMM	739:757	Three associated genomic regions, one in chromosome (chr) 1 and two novel associations in chr-8, were identified using combination of LMM and Bayesian sparse LMM.
33276449	5	62	theme	-like	900:904	arg1	Sobic.008G111500					915:930	Sobic.008G111500	915:930	Sobic.008G111500	915:930	All significant SNPs were located within protein coding genes, with SNPs ∼ 52 Mb of chr-8 encoding a Casperian strip membrane protein (CASP)-like protein (Sobic.008G111500) and a heat shock protein (HSP) 90 (Sobic.008G111600) that were highly expressed in reproductive tissues including within the embryo and endosperm.
33276449	5	62	theme	-like	900:904	arg1	protein					906:912	a Casperian strip membrane protein (CASP)-like protein	859:912	a Casperian strip membrane protein (CASP)-like protein (Sobic.008G111500)	859:931	All significant SNPs were located within protein coding genes, with SNPs ∼ 52 Mb of chr-8 encoding a Casperian strip membrane protein (CASP)-like protein (Sobic.008G111500) and a heat shock protein (HSP) 90 (Sobic.008G111600) that were highly expressed in reproductive tissues including within the embryo and endosperm.
33276449	5	62	theme	-like	900:904	arg1	Sobic.008G111600					968:983	Sobic.008G111600	968:983	Sobic.008G111600	968:983	All significant SNPs were located within protein coding genes, with SNPs ∼ 52 Mb of chr-8 encoding a Casperian strip membrane protein (CASP)-like protein (Sobic.008G111500) and a heat shock protein (HSP) 90 (Sobic.008G111600) that were highly expressed in reproductive tissues including within the embryo and endosperm.
33276449	2	63	theme	genetic	250:256	arg1	control					258:264	Unraveling genetic control	239:264	Unraveling genetic control for starch metabolism	239:286	Unraveling genetic control for starch metabolism is important for breeding grains with high starch content.
33276449	5	64	theme	chr-8	844:848	arg1	Mb					838:839	52 Mb	835:839	52 Mb of chr-8 encoding a Casperian strip membrane protein (CASP)-like protein (Sobic.008G111500) and a heat shock protein (HSP) 90 (Sobic.008G111600) that were highly expressed in reproductive tissues including within the embryo and endosperm	835:1077	All significant SNPs were located within protein coding genes, with SNPs ∼ 52 Mb of chr-8 encoding a Casperian strip membrane protein (CASP)-like protein (Sobic.008G111500) and a heat shock protein (HSP) 90 (Sobic.008G111600) that were highly expressed in reproductive tissues including within the embryo and endosperm.
33276449	6	65	with	gene	1109:1112	arg1	network					1124:1130	gene network	1119:1130	gene network of 75 high-confidence first interactors that is enriched for five biochemical pathways including protein processing	1119:1246	The HSP90 is a potential hub gene with gene network of 75 high-confidence first interactors that is enriched for five biochemical pathways including protein processing.
33276449	1	66	from	human	211:215	arg1	energy					166:171	reserve energy	158:171	reserve energy for germination	158:187	Starch accumulated in the endosperm of cereal grains as reserve energy for germination serves as a staple in human and animal nutrition.
33276449	1	66	from	human	211:215	arg1	staple					201:206	staple	201:206	staple	201:206	Starch accumulated in the endosperm of cereal grains as reserve energy for germination serves as a staple in human and animal nutrition.
33276449	2	67	theme	Unraveling	239:248	arg1	control					258:264	Unraveling genetic control	239:264	Unraveling genetic control for starch metabolism	239:286	Unraveling genetic control for starch metabolism is important for breeding grains with high starch content.
33276449	4	68	theme	novel	665:669	arg1	associations					671:682	novel associations	665:682	novel associations	665:682	Three associated genomic regions, one in chromosome (chr) 1 and two novel associations in chr-8, were identified using combination of LMM and Bayesian sparse LMM.
33276449	7	69	from	abundance	1308:1316	arg1	tissues					1334:1340	reproductive tissues	1321:1340	reproductive tissues	1321:1340	The first interactors of HSP90 also showed high transcript abundance in reproductive tissues.
33276449	0	70	theme	Grain	69:73	arg1	Content					82:88	Grain Starch Content	69:88	Grain Starch Content in Sorghum	69:99	Identification of Novel Genomic Associations and Gene Candidates for Grain Starch Content in Sorghum.
33276449	4	71	theme	genomic	614:620	arg1	one					631:633	one	631:633	one	631:633	Three associated genomic regions, one in chromosome (chr) 1 and two novel associations in chr-8, were identified using combination of LMM and Bayesian sparse LMM.
33276449	4	71	theme	genomic	614:620	arg1	regions					622:628	Three associated genomic regions	597:628	Three associated genomic regions	597:628	Three associated genomic regions, one in chromosome (chr) 1 and two novel associations in chr-8, were identified using combination of LMM and Bayesian sparse LMM.
33276449	4	71	theme	genomic	614:620	arg1	two					661:663	two	661:663	two	661:663	Three associated genomic regions, one in chromosome (chr) 1 and two novel associations in chr-8, were identified using combination of LMM and Bayesian sparse LMM.
33276449	4	72	theme	sparse	748:753	arg1	LMM					755:757	Bayesian sparse LMM	739:757	Bayesian sparse LMM	739:757	Three associated genomic regions, one in chromosome (chr) 1 and two novel associations in chr-8, were identified using combination of LMM and Bayesian sparse LMM.
33276449	4	73	theme	associated	603:612	arg1	one					631:633	one	631:633	one	631:633	Three associated genomic regions, one in chromosome (chr) 1 and two novel associations in chr-8, were identified using combination of LMM and Bayesian sparse LMM.
33276449	4	73	theme	associated	603:612	arg1	regions					622:628	Three associated genomic regions	597:628	Three associated genomic regions	597:628	Three associated genomic regions, one in chromosome (chr) 1 and two novel associations in chr-8, were identified using combination of LMM and Bayesian sparse LMM.
33276449	4	73	theme	associated	603:612	arg1	two					661:663	two	661:663	two	661:663	Three associated genomic regions, one in chromosome (chr) 1 and two novel associations in chr-8, were identified using combination of LMM and Bayesian sparse LMM.
33276449	3	74	theme	mixed	484:488	arg1	LMM					498:500	LMM	498:500	LMM	498:500	In this study, we used a sorghum association panel with 389 individuals and 141,557 single nucleotide polymorphisms (SNPs) to fit linear mixed models (LMM) for identifying genomic regions and potential candidate genes associated with starch content.
33276449	3	74	theme	mixed	484:488	arg1	models					490:495	linear mixed models	477:495	linear mixed models (LMM) for identifying genomic regions and potential candidate genes associated with starch content	477:594	In this study, we used a sorghum association panel with 389 individuals and 141,557 single nucleotide polymorphisms (SNPs) to fit linear mixed models (LMM) for identifying genomic regions and potential candidate genes associated with starch content.
33276449	8	75	theme	metabolic	1405:1413	arg1	pathways					1415:1422	intricate metabolic pathways	1395:1422	intricate metabolic pathways	1395:1422	The candidates of this study are likely involved in intricate metabolic pathways and represent candidate gene targets for source-sink activities and drought and heat stress tolerance during grain filling.
33276449	3	76	theme	starch	581:586	arg1	content					588:594	starch content	581:594	starch content	581:594	In this study, we used a sorghum association panel with 389 individuals and 141,557 single nucleotide polymorphisms (SNPs) to fit linear mixed models (LMM) for identifying genomic regions and potential candidate genes associated with starch content.
33276449	1	77	theme	cereal	141:146	arg1	grains					148:153	cereal grains	141:153	cereal grains	141:153	Starch accumulated in the endosperm of cereal grains as reserve energy for germination serves as a staple in human and animal nutrition.
33276449	8	78	theme	grain	1533:1537	arg1	filling					1539:1545	grain filling	1533:1545	grain filling	1533:1545	The candidates of this study are likely involved in intricate metabolic pathways and represent candidate gene targets for source-sink activities and drought and heat stress tolerance during grain filling.
32672838	2	0	theme	residual	767:774	arg1	chains					783:788	the residual glycan chains	763:788	the residual glycan chains	763:788	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	4	1	theme	acid	1274:1277	arg1	release					1256:1262	the selective release	1242:1262	the selective release of sialic acid from AGP	1242:1286	The presence of other sugars was not detected by 1 H NMR and HPAEC-PAD, suggesting that HPAs hydrolyze only the terminal glycosidic bond in the glycoprotein, resulting in the selective release of sialic acid from AGP.
32672838	2	2	with	removal	687:693	arg1	destruction					728:738	no observed destruction	716:738	no observed destruction of the protein core	716:758	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	3	3	theme	suitable	953:960	arg1	method					946:951	the reported method	933:951	the reported method suitable for the analysis of sialic acid modifications which play an important role in numerous biological processes	933:1068	The 1 H NMR spectroscopy confirmed that the released sialic acids preserve intact structure upon their excision from the protein, which makes the reported method suitable for the analysis of sialic acid modifications which play an important role in numerous biological processes.
32672838	5	4	theme	mineral	1480:1486	arg1	acids					1488:1492	conventional mineral acids	1467:1492	conventional mineral acids	1467:1492	The kinetic results have shown that under equal temperature and pH conditions, the hydrolysis of the terminal glucosidic bond occurred faster in the presence of HPAs compared to conventional mineral acids.
32672838	2	5	with	incubation	547:556	arg1	HPAs					578:581	HPAs	578:581	HPAs	578:581	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	3	6	theme	reported	937:944	arg1	method					946:951	the reported method	933:951	the reported method suitable for the analysis of sialic acid modifications which play an important role in numerous biological processes	933:1068	The 1 H NMR spectroscopy confirmed that the released sialic acids preserve intact structure upon their excision from the protein, which makes the reported method suitable for the analysis of sialic acid modifications which play an important role in numerous biological processes.
32672838	2	7	theme	sialic	698:703	arg1	acids					705:709	sialic acids	698:709	sialic acids	698:709	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	4	8	theme	selective	1246:1254	arg1	release					1256:1262	the selective release	1242:1262	the selective release of sialic acid from AGP	1242:1286	The presence of other sugars was not detected by 1 H NMR and HPAEC-PAD, suggesting that HPAs hydrolyze only the terminal glycosidic bond in the glycoprotein, resulting in the selective release of sialic acid from AGP.
32672838	1	9	theme	Wells-Dawson	172:183	arg1	range					152:156	a range	150:156	a range of Keggin and Wells-Dawson type heteropolyacids (HPAs): H3 PW12 O40 H4 SiW12 O40 , H3 PMo12 O40	150:252	The reactivity of a range of Keggin and Wells-Dawson type heteropolyacids (HPAs): H3 PW12 O40 H4 SiW12 O40 , H3 PMo12 O40 , K6 P2 W18 O62 , and NaH2 W12 O4 , towards the heavily glycosylated α-1-acid glycoprotein (AGP) is reported.
32672838	5	10	theme	equal	1331:1335	arg1	temperature					1337:1347	equal temperature	1331:1347	equal temperature	1331:1347	The kinetic results have shown that under equal temperature and pH conditions, the hydrolysis of the terminal glucosidic bond occurred faster in the presence of HPAs compared to conventional mineral acids.
32672838	7	11	theme	anionic	1907:1913	arg1	cluster					1919:1925	anionic HPA cluster	1907:1925	anionic HPA cluster	1907:1925	The Trp fluorescence and CD spectroscopy show that non-covalent interaction between HPA and protein takes place in solution which could lead to stabilization of the sialosyl cation that is formed during the glycosidic bond hydrolysis by anionic HPA cluster.
32672838	7	12	theme	Trp	1674:1676	arg1	fluorescence					1678:1689	The Trp fluorescence	1670:1689	The Trp fluorescence	1670:1689	The Trp fluorescence and CD spectroscopy show that non-covalent interaction between HPA and protein takes place in solution which could lead to stabilization of the sialosyl cation that is formed during the glycosidic bond hydrolysis by anionic HPA cluster.
32672838	2	13	theme	observed	719:726	arg1	destruction					728:738	no observed destruction	716:738	no observed destruction of the protein core	716:758	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	1	14	theme	glycosylated	310:321	arg1	glycoprotein					332:343	the heavily glycosylated α-1-acid glycoprotein	298:343	the heavily glycosylated α-1-acid glycoprotein (AGP)	298:349	The reactivity of a range of Keggin and Wells-Dawson type heteropolyacids (HPAs): H3 PW12 O40 H4 SiW12 O40 , H3 PMo12 O40 , K6 P2 W18 O62 , and NaH2 W12 O4 , towards the heavily glycosylated α-1-acid glycoprotein (AGP) is reported.
32672838	1	14	theme	glycosylated	310:321	arg1	AGP					346:348	AGP	346:348	AGP	346:348	The reactivity of a range of Keggin and Wells-Dawson type heteropolyacids (HPAs): H3 PW12 O40 H4 SiW12 O40 , H3 PMo12 O40 , K6 P2 W18 O62 , and NaH2 W12 O4 , towards the heavily glycosylated α-1-acid glycoprotein (AGP) is reported.
32672838	2	15	theme	amperometric	496:507	arg1	detection					509:517	pulsed amperometric detection	489:517	pulsed amperometric detection (HPAEC-PAD)	489:529	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	2	15	theme	amperometric	496:507	arg1	HPAEC-PAD					520:528	HPAEC-PAD	520:528	HPAEC-PAD	520:528	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	6	16	theme	sialic	1610:1615	arg1	acids					1617:1621	sialic acids	1610:1621	sialic acids	1610:1621	The observed rate constants were in the range 6,7×10-2 -11,9×10-2 min-1 and the complete and selective excision of sialic acids could be achieved within 60 min of incubation.
32672838	7	17	theme	glycosidic	1877:1886	arg1	hydrolysis					1893:1902	the glycosidic bond hydrolysis	1873:1902	the glycosidic bond hydrolysis by anionic HPA cluster	1873:1925	The Trp fluorescence and CD spectroscopy show that non-covalent interaction between HPA and protein takes place in solution which could lead to stabilization of the sialosyl cation that is formed during the glycosidic bond hydrolysis by anionic HPA cluster.
32672838	6	18	theme	observed	1499:1506	arg1	constants					1513:1521	The observed rate constants	1495:1521	The observed rate constants	1495:1521	The observed rate constants were in the range 6,7×10-2 -11,9×10-2 min-1 and the complete and selective excision of sialic acids could be achieved within 60 min of incubation.
32672838	2	19	theme	bond	646:649	arg1	hydrolysis					612:621	complete hydrolysis	603:621	complete hydrolysis of terminal glycosidic bond	603:649	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	2	20	theme	high-performance	437:452	arg1	chromatography					469:482	high-performance anion exchange chromatography	437:482	high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD)	437:529	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	4	21	theme	other	1087:1091	arg1	sugars					1093:1098	other sugars	1087:1098	other sugars	1087:1098	The presence of other sugars was not detected by 1 H NMR and HPAEC-PAD, suggesting that HPAs hydrolyze only the terminal glycosidic bond in the glycoprotein, resulting in the selective release of sialic acid from AGP.
32672838	1	22	theme	range	152:156	arg1	reactivity					136:145	The reactivity	132:145	The reactivity of a range of Keggin and Wells-Dawson type heteropolyacids (HPAs): H3 PW12 O40 H4 SiW12 O40 , H3 PMo12 O40	132:252	The reactivity of a range of Keggin and Wells-Dawson type heteropolyacids (HPAs): H3 PW12 O40 H4 SiW12 O40 , H3 PMo12 O40 , K6 P2 W18 O62 , and NaH2 W12 O4 , towards the heavily glycosylated α-1-acid glycoprotein (AGP) is reported.
32672838	1	22	theme	range	152:156	arg1	O4					285:286	NaH2 W12 O4	276:286	NaH2 W12 O4	276:286	The reactivity of a range of Keggin and Wells-Dawson type heteropolyacids (HPAs): H3 PW12 O40 H4 SiW12 O40 , H3 PMo12 O40 , K6 P2 W18 O62 , and NaH2 W12 O4 , towards the heavily glycosylated α-1-acid glycoprotein (AGP) is reported.
32672838	1	22	theme	range	152:156	arg1	O62					266:268	K6 P2 W18 O62	256:268	K6 P2 W18 O62	256:268	The reactivity of a range of Keggin and Wells-Dawson type heteropolyacids (HPAs): H3 PW12 O40 H4 SiW12 O40 , H3 PMo12 O40 , K6 P2 W18 O62 , and NaH2 W12 O4 , towards the heavily glycosylated α-1-acid glycoprotein (AGP) is reported.
32672838	6	23	theme	incubation	1658:1667	arg1	60 min					1648:1653	60 min	1648:1653	60 min of incubation	1648:1667	The observed rate constants were in the range 6,7×10-2 -11,9×10-2 min-1 and the complete and selective excision of sialic acids could be achieved within 60 min of incubation.
32672838	5	24	theme	terminal	1390:1397	arg1	bond					1410:1413	the terminal glucosidic bond	1386:1413	the terminal glucosidic bond	1386:1413	The kinetic results have shown that under equal temperature and pH conditions, the hydrolysis of the terminal glucosidic bond occurred faster in the presence of HPAs compared to conventional mineral acids.
32672838	1	25	theme	W12	281:283	arg1	O4					285:286	NaH2 W12 O4	276:286	NaH2 W12 O4	276:286	The reactivity of a range of Keggin and Wells-Dawson type heteropolyacids (HPAs): H3 PW12 O40 H4 SiW12 O40 , H3 PMo12 O40 , K6 P2 W18 O62 , and NaH2 W12 O4 , towards the heavily glycosylated α-1-acid glycoprotein (AGP) is reported.
32672838	1	26	theme	Keggin	161:166	arg1	range					152:156	a range	150:156	a range of Keggin and Wells-Dawson type heteropolyacids (HPAs): H3 PW12 O40 H4 SiW12 O40 , H3 PMo12 O40	150:252	The reactivity of a range of Keggin and Wells-Dawson type heteropolyacids (HPAs): H3 PW12 O40 H4 SiW12 O40 , H3 PMo12 O40 , K6 P2 W18 O62 , and NaH2 W12 O4 , towards the heavily glycosylated α-1-acid glycoprotein (AGP) is reported.
32672838	3	27	theme	H NMR	797:801	arg1	spectroscopy					803:814	The 1 H NMR spectroscopy	791:814	The 1 H NMR spectroscopy	791:814	The 1 H NMR spectroscopy confirmed that the released sialic acids preserve intact structure upon their excision from the protein, which makes the reported method suitable for the analysis of sialic acid modifications which play an important role in numerous biological processes.
32672838	5	28	theme	bond	1410:1413	arg1	hydrolysis					1372:1381	the hydrolysis	1368:1381	the hydrolysis of the terminal glucosidic bond	1368:1413	The kinetic results have shown that under equal temperature and pH conditions, the hydrolysis of the terminal glucosidic bond occurred faster in the presence of HPAs compared to conventional mineral acids.
32672838	3	29	theme	acid	989:992	arg1	modifications					994:1006	sialic acid modifications	982:1006	sialic acid modifications which play an important role in numerous biological processes	982:1068	The 1 H NMR spectroscopy confirmed that the released sialic acids preserve intact structure upon their excision from the protein, which makes the reported method suitable for the analysis of sialic acid modifications which play an important role in numerous biological processes.
32672838	6	30	theme	-11,9×10-2	1550:1559	arg1	min-1					1561:1565	the range 6,7×10-2 -11,9×10-2 min-1	1531:1565	the range 6,7×10-2 -11,9×10-2 min-1	1531:1565	The observed rate constants were in the range 6,7×10-2 -11,9×10-2 min-1 and the complete and selective excision of sialic acids could be achieved within 60 min of incubation.
32672838	2	31	with	chains	783:788	arg1	destruction					728:738	no observed destruction	716:738	no observed destruction of the protein core	716:758	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	3	32	theme	sialic	844:849	arg1	acids					851:855	the released sialic acids	831:855	the released sialic acids	831:855	The 1 H NMR spectroscopy confirmed that the released sialic acids preserve intact structure upon their excision from the protein, which makes the reported method suitable for the analysis of sialic acid modifications which play an important role in numerous biological processes.
32672838	2	33	theme	exchange	460:467	arg1	chromatography					469:482	high-performance anion exchange chromatography	437:482	high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD)	437:529	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	0	34	theme	Type	110:113	arg1	Heteropolyacids					115:129	Type Heteropolyacids	110:129	Type Heteropolyacids	110:129	Selective Hydrolysis of Terminal Glycosidic Bond in α-1-Acid Glycoprotein Promoted by Keggin and Wells-Dawson Type Heteropolyacids.
32672838	6	35	theme	range	1535:1539	arg1	min-1					1561:1565	the range 6,7×10-2 -11,9×10-2 min-1	1531:1565	the range 6,7×10-2 -11,9×10-2 min-1	1531:1565	The observed rate constants were in the range 6,7×10-2 -11,9×10-2 min-1 and the complete and selective excision of sialic acids could be achieved within 60 min of incubation.
32672838	2	36	theme	sulfate	379:385	arg1	SDS-PAGE					423:430	SDS-PAGE	423:430	SDS-PAGE	423:430	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	2	36	theme	sulfate	379:385	arg1	electrophoresis					406:420	Sodium dodecyl sulfate polyacrylamide gel electrophoresis	364:420	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)	364:431	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	2	37	theme	terminal	626:633	arg1	bond					646:649	terminal glycosidic bond	626:649	terminal glycosidic bond	626:649	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	0	38	theme	Selective	0:8	arg1	Hydrolysis					10:19	Selective Hydrolysis	0:19	Selective Hydrolysis of Terminal Glycosidic Bond in α-1-Acid Glycoprotein	0:72	Selective Hydrolysis of Terminal Glycosidic Bond in α-1-Acid Glycoprotein Promoted by Keggin and Wells-Dawson Type Heteropolyacids.
32672838	7	39	theme	sialosyl	1835:1842	arg1	cation					1844:1849	the sialosyl cation	1831:1849	the sialosyl cation that is formed during the glycosidic bond hydrolysis by anionic HPA cluster	1831:1925	The Trp fluorescence and CD spectroscopy show that non-covalent interaction between HPA and protein takes place in solution which could lead to stabilization of the sialosyl cation that is formed during the glycosidic bond hydrolysis by anionic HPA cluster.
32672838	2	40	theme	Sodium	364:369	arg1	SDS-PAGE					423:430	SDS-PAGE	423:430	SDS-PAGE	423:430	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	2	40	theme	Sodium	364:369	arg1	electrophoresis					406:420	Sodium dodecyl sulfate polyacrylamide gel electrophoresis	364:420	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)	364:431	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	0	41	theme	Glycosidic	33:42	arg1	Bond					44:47	Terminal Glycosidic Bond	24:47	Terminal Glycosidic Bond	24:47	Selective Hydrolysis of Terminal Glycosidic Bond in α-1-Acid Glycoprotein Promoted by Keggin and Wells-Dawson Type Heteropolyacids.
32672838	1	42	theme	P2	259:260	arg1	O62					266:268	K6 P2 W18 O62	256:268	K6 P2 W18 O62	256:268	The reactivity of a range of Keggin and Wells-Dawson type heteropolyacids (HPAs): H3 PW12 O40 H4 SiW12 O40 , H3 PMo12 O40 , K6 P2 W18 O62 , and NaH2 W12 O4 , towards the heavily glycosylated α-1-acid glycoprotein (AGP) is reported.
32672838	3	43	theme	numerous	1040:1047	arg1	processes					1060:1068	numerous biological processes	1040:1068	numerous biological processes	1040:1068	The 1 H NMR spectroscopy confirmed that the released sialic acids preserve intact structure upon their excision from the protein, which makes the reported method suitable for the analysis of sialic acid modifications which play an important role in numerous biological processes.
32672838	2	44	theme	gel	402:404	arg1	SDS-PAGE					423:430	SDS-PAGE	423:430	SDS-PAGE	423:430	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	2	44	theme	gel	402:404	arg1	electrophoresis					406:420	Sodium dodecyl sulfate polyacrylamide gel electrophoresis	364:420	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)	364:431	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	4	45	from	bond	1203:1206	arg1	glycoprotein					1215:1226	the glycoprotein	1211:1226	the glycoprotein	1211:1226	The presence of other sugars was not detected by 1 H NMR and HPAEC-PAD, suggesting that HPAs hydrolyze only the terminal glycosidic bond in the glycoprotein, resulting in the selective release of sialic acid from AGP.
32672838	2	46	theme	core	755:758	arg1	destruction					728:738	no observed destruction	716:738	no observed destruction of the protein core	716:758	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	1	47	gly	glycosylated	310:321	arg1	glycoprotein					332:343	the heavily glycosylated α-1-acid glycoprotein	298:343	the heavily glycosylated α-1-acid glycoprotein (AGP)	298:349	The reactivity of a range of Keggin and Wells-Dawson type heteropolyacids (HPAs): H3 PW12 O40 H4 SiW12 O40 , H3 PMo12 O40 , K6 P2 W18 O62 , and NaH2 W12 O4 , towards the heavily glycosylated α-1-acid glycoprotein (AGP) is reported.
32672838	1	47	gly	glycosylated	310:321	arg1	AGP					346:348	AGP	346:348	AGP	346:348	The reactivity of a range of Keggin and Wells-Dawson type heteropolyacids (HPAs): H3 PW12 O40 H4 SiW12 O40 , H3 PMo12 O40 , K6 P2 W18 O62 , and NaH2 W12 O4 , towards the heavily glycosylated α-1-acid glycoprotein (AGP) is reported.
32672838	4	48	theme	glycosidic	1192:1201	arg1	bond					1203:1206	only the terminal glycosidic bond	1174:1206	only the terminal glycosidic bond in the glycoprotein	1174:1226	The presence of other sugars was not detected by 1 H NMR and HPAEC-PAD, suggesting that HPAs hydrolyze only the terminal glycosidic bond in the glycoprotein, resulting in the selective release of sialic acid from AGP.
32672838	1	49	gly	glycoprotein	332:343	arg1	glycoprotein					332:343	the heavily glycosylated α-1-acid glycoprotein	298:343	the heavily glycosylated α-1-acid glycoprotein (AGP)	298:349	The reactivity of a range of Keggin and Wells-Dawson type heteropolyacids (HPAs): H3 PW12 O40 H4 SiW12 O40 , H3 PMo12 O40 , K6 P2 W18 O62 , and NaH2 W12 O4 , towards the heavily glycosylated α-1-acid glycoprotein (AGP) is reported.
32672838	1	49	gly	glycoprotein	332:343	arg1	AGP					346:348	AGP	346:348	AGP	346:348	The reactivity of a range of Keggin and Wells-Dawson type heteropolyacids (HPAs): H3 PW12 O40 H4 SiW12 O40 , H3 PMo12 O40 , K6 P2 W18 O62 , and NaH2 W12 O4 , towards the heavily glycosylated α-1-acid glycoprotein (AGP) is reported.
32672838	7	50	theme	non-covalent	1721:1732	arg1	interaction					1734:1744	non-covalent interaction	1721:1744	non-covalent interaction between HPA and protein	1721:1768	The Trp fluorescence and CD spectroscopy show that non-covalent interaction between HPA and protein takes place in solution which could lead to stabilization of the sialosyl cation that is formed during the glycosidic bond hydrolysis by anionic HPA cluster.
32672838	2	51	theme	glycan	776:781	arg1	chains					783:788	the residual glycan chains	763:788	the residual glycan chains	763:788	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	7	52	theme	cation	1844:1849	arg1	stabilization					1814:1826	stabilization	1814:1826	stabilization of the sialosyl cation that is formed during the glycosidic bond hydrolysis by anionic HPA cluster	1814:1925	The Trp fluorescence and CD spectroscopy show that non-covalent interaction between HPA and protein takes place in solution which could lead to stabilization of the sialosyl cation that is formed during the glycosidic bond hydrolysis by anionic HPA cluster.
32672838	5	53	theme	conventional	1467:1478	arg1	acids					1488:1492	conventional mineral acids	1467:1492	conventional mineral acids	1467:1492	The kinetic results have shown that under equal temperature and pH conditions, the hydrolysis of the terminal glucosidic bond occurred faster in the presence of HPAs compared to conventional mineral acids.
32672838	4	54	theme	sialic	1267:1272	arg1	acid					1274:1277	sialic acid	1267:1277	sialic acid from AGP	1267:1286	The presence of other sugars was not detected by 1 H NMR and HPAEC-PAD, suggesting that HPAs hydrolyze only the terminal glycosidic bond in the glycoprotein, resulting in the selective release of sialic acid from AGP.
32672838	5	55	theme	pH	1353:1354	arg1	conditions					1356:1365	equal temperature and pH conditions	1331:1365	equal temperature and pH conditions	1331:1365	The kinetic results have shown that under equal temperature and pH conditions, the hydrolysis of the terminal glucosidic bond occurred faster in the presence of HPAs compared to conventional mineral acids.
32672838	7	56	theme	CD	1695:1696	arg1	spectroscopy					1698:1709	CD spectroscopy	1695:1709	CD spectroscopy	1695:1709	The Trp fluorescence and CD spectroscopy show that non-covalent interaction between HPA and protein takes place in solution which could lead to stabilization of the sialosyl cation that is formed during the glycosidic bond hydrolysis by anionic HPA cluster.
32672838	2	57	theme	acids	705:709	arg1	removal					687:693	the removal	683:693	the removal of sialic acids with no observed destruction of the protein core	683:758	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	2	57	theme	acids	705:709	arg1	chains					783:788	the residual glycan chains	763:788	the residual glycan chains	763:788	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	1	58	theme	type	185:188	arg1	heteropolyacids					190:204	type heteropolyacids	185:204	type heteropolyacids	185:204	The reactivity of a range of Keggin and Wells-Dawson type heteropolyacids (HPAs): H3 PW12 O40 H4 SiW12 O40 , H3 PMo12 O40 , K6 P2 W18 O62 , and NaH2 W12 O4 , towards the heavily glycosylated α-1-acid glycoprotein (AGP) is reported.
32672838	2	59	theme	protein	565:571	arg1	incubation					547:556	incubation	547:556	incubation of the protein with HPAs at 80 °C and pH 2.8	547:601	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	5	60	theme	temperature	1337:1347	arg1	conditions					1356:1365	equal temperature and pH conditions	1331:1365	equal temperature and pH conditions	1331:1365	The kinetic results have shown that under equal temperature and pH conditions, the hydrolysis of the terminal glucosidic bond occurred faster in the presence of HPAs compared to conventional mineral acids.
32672838	7	61	theme	HPA	1915:1917	arg1	cluster					1919:1925	anionic HPA cluster	1907:1925	anionic HPA cluster	1907:1925	The Trp fluorescence and CD spectroscopy show that non-covalent interaction between HPA and protein takes place in solution which could lead to stabilization of the sialosyl cation that is formed during the glycosidic bond hydrolysis by anionic HPA cluster.
32672838	6	62	theme	acids	1617:1621	arg1	excision					1598:1605	the complete and selective excision	1571:1605	the complete and selective excision of sialic acids	1571:1621	The observed rate constants were in the range 6,7×10-2 -11,9×10-2 min-1 and the complete and selective excision of sialic acids could be achieved within 60 min of incubation.
32672838	1	63	theme	α-1-acid	323:330	arg1	glycoprotein					332:343	the heavily glycosylated α-1-acid glycoprotein	298:343	the heavily glycosylated α-1-acid glycoprotein (AGP)	298:349	The reactivity of a range of Keggin and Wells-Dawson type heteropolyacids (HPAs): H3 PW12 O40 H4 SiW12 O40 , H3 PMo12 O40 , K6 P2 W18 O62 , and NaH2 W12 O4 , towards the heavily glycosylated α-1-acid glycoprotein (AGP) is reported.
32672838	1	63	theme	α-1-acid	323:330	arg1	AGP					346:348	AGP	346:348	AGP	346:348	The reactivity of a range of Keggin and Wells-Dawson type heteropolyacids (HPAs): H3 PW12 O40 H4 SiW12 O40 , H3 PMo12 O40 , K6 P2 W18 O62 , and NaH2 W12 O4 , towards the heavily glycosylated α-1-acid glycoprotein (AGP) is reported.
32672838	2	64	theme	pulsed	489:494	arg1	detection					509:517	pulsed amperometric detection	489:517	pulsed amperometric detection (HPAEC-PAD)	489:529	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	2	64	theme	pulsed	489:494	arg1	HPAEC-PAD					520:528	HPAEC-PAD	520:528	HPAEC-PAD	520:528	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	4	65	from	AGP	1284:1286	arg1	release					1256:1262	the selective release	1242:1262	the selective release of sialic acid from AGP	1242:1286	The presence of other sugars was not detected by 1 H NMR and HPAEC-PAD, suggesting that HPAs hydrolyze only the terminal glycosidic bond in the glycoprotein, resulting in the selective release of sialic acid from AGP.
32672838	4	65	from	AGP	1284:1286	arg1	acid					1274:1277	sialic acid	1267:1277	sialic acid from AGP	1267:1286	The presence of other sugars was not detected by 1 H NMR and HPAEC-PAD, suggesting that HPAs hydrolyze only the terminal glycosidic bond in the glycoprotein, resulting in the selective release of sialic acid from AGP.
32672838	6	66	theme	rate	1508:1511	arg1	constants					1513:1521	The observed rate constants	1495:1521	The observed rate constants	1495:1521	The observed rate constants were in the range 6,7×10-2 -11,9×10-2 min-1 and the complete and selective excision of sialic acids could be achieved within 60 min of incubation.
32672838	7	67	theme	bond	1888:1891	arg1	hydrolysis					1893:1902	the glycosidic bond hydrolysis	1873:1902	the glycosidic bond hydrolysis by anionic HPA cluster	1873:1925	The Trp fluorescence and CD spectroscopy show that non-covalent interaction between HPA and protein takes place in solution which could lead to stabilization of the sialosyl cation that is formed during the glycosidic bond hydrolysis by anionic HPA cluster.
32672838	6	68	theme	selective	1588:1596	arg1	excision					1598:1605	the complete and selective excision	1571:1605	the complete and selective excision of sialic acids	1571:1621	The observed rate constants were in the range 6,7×10-2 -11,9×10-2 min-1 and the complete and selective excision of sialic acids could be achieved within 60 min of incubation.
32672838	2	69	from	80 °C	586:590	arg1	incubation					547:556	incubation	547:556	incubation of the protein with HPAs at 80 °C and pH 2.8	547:601	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	6	70	theme	complete	1575:1582	arg1	excision					1598:1605	the complete and selective excision	1571:1605	the complete and selective excision of sialic acids	1571:1621	The observed rate constants were in the range 6,7×10-2 -11,9×10-2 min-1 and the complete and selective excision of sialic acids could be achieved within 60 min of incubation.
32672838	4	71	theme	sugars	1093:1098	arg1	presence					1075:1082	The presence	1071:1082	The presence of other sugars	1071:1098	The presence of other sugars was not detected by 1 H NMR and HPAEC-PAD, suggesting that HPAs hydrolyze only the terminal glycosidic bond in the glycoprotein, resulting in the selective release of sialic acid from AGP.
32672838	2	72	from	pH 2.8	596:601	arg1	incubation					547:556	incubation	547:556	incubation of the protein with HPAs at 80 °C and pH 2.8	547:601	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	4	73	gly	glycoprotein	1215:1226	arg1	glycoprotein					1215:1226	the glycoprotein	1211:1226	the glycoprotein	1211:1226	The presence of other sugars was not detected by 1 H NMR and HPAEC-PAD, suggesting that HPAs hydrolyze only the terminal glycosidic bond in the glycoprotein, resulting in the selective release of sialic acid from AGP.
32672838	1	74	theme	NaH2	276:279	arg1	O4					285:286	NaH2 W12 O4	276:286	NaH2 W12 O4	276:286	The reactivity of a range of Keggin and Wells-Dawson type heteropolyacids (HPAs): H3 PW12 O40 H4 SiW12 O40 , H3 PMo12 O40 , K6 P2 W18 O62 , and NaH2 W12 O4 , towards the heavily glycosylated α-1-acid glycoprotein (AGP) is reported.
32672838	3	75	theme	modifications	994:1006	arg1	analysis					970:977	the analysis	966:977	the analysis of sialic acid modifications which play an important role in numerous biological processes	966:1068	The 1 H NMR spectroscopy confirmed that the released sialic acids preserve intact structure upon their excision from the protein, which makes the reported method suitable for the analysis of sialic acid modifications which play an important role in numerous biological processes.
32672838	2	76	with	electrophoresis	406:420	arg1	detection					509:517	pulsed amperometric detection	489:517	pulsed amperometric detection (HPAEC-PAD)	489:529	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	2	76	with	electrophoresis	406:420	arg1	HPAEC-PAD					520:528	HPAEC-PAD	520:528	HPAEC-PAD	520:528	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	0	77	from	Hydrolysis	10:19	arg1	Glycoprotein					61:72	α-1-Acid Glycoprotein	52:72	α-1-Acid Glycoprotein	52:72	Selective Hydrolysis of Terminal Glycosidic Bond in α-1-Acid Glycoprotein Promoted by Keggin and Wells-Dawson Type Heteropolyacids.
32672838	5	78	theme	glucosidic	1399:1408	arg1	bond					1410:1413	the terminal glucosidic bond	1386:1413	the terminal glucosidic bond	1386:1413	The kinetic results have shown that under equal temperature and pH conditions, the hydrolysis of the terminal glucosidic bond occurred faster in the presence of HPAs compared to conventional mineral acids.
32672838	2	79	theme	complete	603:610	arg1	hydrolysis					612:621	complete hydrolysis	603:621	complete hydrolysis of terminal glycosidic bond	603:649	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	3	80	theme	sialic	982:987	arg1	modifications					994:1006	sialic acid modifications	982:1006	sialic acid modifications which play an important role in numerous biological processes	982:1068	The 1 H NMR spectroscopy confirmed that the released sialic acids preserve intact structure upon their excision from the protein, which makes the reported method suitable for the analysis of sialic acid modifications which play an important role in numerous biological processes.
32672838	4	81	from	release	1256:1262	arg1	AGP					1284:1286	AGP	1284:1286	AGP	1284:1286	The presence of other sugars was not detected by 1 H NMR and HPAEC-PAD, suggesting that HPAs hydrolyze only the terminal glycosidic bond in the glycoprotein, resulting in the selective release of sialic acid from AGP.
32672838	3	82	theme	important	1022:1030	arg1	role					1032:1035	an important role	1019:1035	an important role	1019:1035	The 1 H NMR spectroscopy confirmed that the released sialic acids preserve intact structure upon their excision from the protein, which makes the reported method suitable for the analysis of sialic acid modifications which play an important role in numerous biological processes.
32672838	6	83	theme	6,7×10-2	1541:1548	arg1	min-1					1561:1565	the range 6,7×10-2 -11,9×10-2 min-1	1531:1565	the range 6,7×10-2 -11,9×10-2 min-1	1531:1565	The observed rate constants were in the range 6,7×10-2 -11,9×10-2 min-1 and the complete and selective excision of sialic acids could be achieved within 60 min of incubation.
32672838	3	84	from	protein	912:918	arg1	excision					894:901	their excision	888:901	their excision from the protein, which makes the reported method suitable for the analysis of sialic acid modifications which play an important role in numerous biological processes	888:1068	The 1 H NMR spectroscopy confirmed that the released sialic acids preserve intact structure upon their excision from the protein, which makes the reported method suitable for the analysis of sialic acid modifications which play an important role in numerous biological processes.
32672838	2	85	theme	anion	454:458	arg1	chromatography					469:482	high-performance anion exchange chromatography	437:482	high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD)	437:529	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	2	86	theme	glycosidic	635:644	arg1	bond					646:649	terminal glycosidic bond	626:649	terminal glycosidic bond	626:649	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	0	87	dep	Keggin	86:91	arg1	Heteropolyacids					115:129	Type Heteropolyacids	110:129	Type Heteropolyacids	110:129	Selective Hydrolysis of Terminal Glycosidic Bond in α-1-Acid Glycoprotein Promoted by Keggin and Wells-Dawson Type Heteropolyacids.
32672838	3	88	theme	released	835:842	arg1	acids					851:855	the released sialic acids	831:855	the released sialic acids	831:855	The 1 H NMR spectroscopy confirmed that the released sialic acids preserve intact structure upon their excision from the protein, which makes the reported method suitable for the analysis of sialic acid modifications which play an important role in numerous biological processes.
32672838	2	89	theme	dodecyl	371:377	arg1	SDS-PAGE					423:430	SDS-PAGE	423:430	SDS-PAGE	423:430	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	2	89	theme	dodecyl	371:377	arg1	electrophoresis					406:420	Sodium dodecyl sulfate polyacrylamide gel electrophoresis	364:420	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)	364:431	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	1	90	dep	Keggin	161:166	arg1	heteropolyacids					190:204	type heteropolyacids	185:204	type heteropolyacids	185:204	The reactivity of a range of Keggin and Wells-Dawson type heteropolyacids (HPAs): H3 PW12 O40 H4 SiW12 O40 , H3 PMo12 O40 , K6 P2 W18 O62 , and NaH2 W12 O4 , towards the heavily glycosylated α-1-acid glycoprotein (AGP) is reported.
32672838	1	90	dep	Keggin	161:166	arg1	O40					250:252	O40	250:252	O40	250:252	The reactivity of a range of Keggin and Wells-Dawson type heteropolyacids (HPAs): H3 PW12 O40 H4 SiW12 O40 , H3 PMo12 O40 , K6 P2 W18 O62 , and NaH2 W12 O4 , towards the heavily glycosylated α-1-acid glycoprotein (AGP) is reported.
32672838	1	90	dep	Keggin	161:166	arg1	H3					241:242	H3	241:242	H3	241:242	The reactivity of a range of Keggin and Wells-Dawson type heteropolyacids (HPAs): H3 PW12 O40 H4 SiW12 O40 , H3 PMo12 O40 , K6 P2 W18 O62 , and NaH2 W12 O4 , towards the heavily glycosylated α-1-acid glycoprotein (AGP) is reported.
32672838	0	91	theme	Terminal	24:31	arg1	Bond					44:47	Terminal Glycosidic Bond	24:47	Terminal Glycosidic Bond	24:47	Selective Hydrolysis of Terminal Glycosidic Bond in α-1-Acid Glycoprotein Promoted by Keggin and Wells-Dawson Type Heteropolyacids.
32672838	3	92	theme	biological	1049:1058	arg1	processes					1060:1068	numerous biological processes	1040:1068	numerous biological processes	1040:1068	The 1 H NMR spectroscopy confirmed that the released sialic acids preserve intact structure upon their excision from the protein, which makes the reported method suitable for the analysis of sialic acid modifications which play an important role in numerous biological processes.
32672838	2	93	with	chromatography	469:482	arg1	detection					509:517	pulsed amperometric detection	489:517	pulsed amperometric detection (HPAEC-PAD)	489:529	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	2	93	with	chromatography	469:482	arg1	HPAEC-PAD					520:528	HPAEC-PAD	520:528	HPAEC-PAD	520:528	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	5	94	theme	HPAs	1450:1453	arg1	presence					1438:1445	the presence	1434:1445	the presence of HPAs	1434:1453	The kinetic results have shown that under equal temperature and pH conditions, the hydrolysis of the terminal glucosidic bond occurred faster in the presence of HPAs compared to conventional mineral acids.
32672838	0	95	theme	Bond	44:47	arg1	Hydrolysis					10:19	Selective Hydrolysis	0:19	Selective Hydrolysis of Terminal Glycosidic Bond in α-1-Acid Glycoprotein	0:72	Selective Hydrolysis of Terminal Glycosidic Bond in α-1-Acid Glycoprotein Promoted by Keggin and Wells-Dawson Type Heteropolyacids.
32672838	1	96	theme	K6	256:257	arg1	O62					266:268	K6 P2 W18 O62	256:268	K6 P2 W18 O62	256:268	The reactivity of a range of Keggin and Wells-Dawson type heteropolyacids (HPAs): H3 PW12 O40 H4 SiW12 O40 , H3 PMo12 O40 , K6 P2 W18 O62 , and NaH2 W12 O4 , towards the heavily glycosylated α-1-acid glycoprotein (AGP) is reported.
32672838	3	97	theme	intact	866:871	arg1	structure					873:881	intact structure	866:881	intact structure	866:881	The 1 H NMR spectroscopy confirmed that the released sialic acids preserve intact structure upon their excision from the protein, which makes the reported method suitable for the analysis of sialic acid modifications which play an important role in numerous biological processes.
32672838	5	98	theme	kinetic	1293:1299	arg1	results					1301:1307	The kinetic results	1289:1307	The kinetic results	1289:1307	The kinetic results have shown that under equal temperature and pH conditions, the hydrolysis of the terminal glucosidic bond occurred faster in the presence of HPAs compared to conventional mineral acids.
32672838	0	99	theme	α-1-Acid	52:59	arg1	Glycoprotein					61:72	α-1-Acid Glycoprotein	52:72	α-1-Acid Glycoprotein	52:72	Selective Hydrolysis of Terminal Glycosidic Bond in α-1-Acid Glycoprotein Promoted by Keggin and Wells-Dawson Type Heteropolyacids.
32672838	1	100	theme	W18	262:264	arg1	O62					266:268	K6 P2 W18 O62	256:268	K6 P2 W18 O62	256:268	The reactivity of a range of Keggin and Wells-Dawson type heteropolyacids (HPAs): H3 PW12 O40 H4 SiW12 O40 , H3 PMo12 O40 , K6 P2 W18 O62 , and NaH2 W12 O4 , towards the heavily glycosylated α-1-acid glycoprotein (AGP) is reported.
32672838	2	101	theme	polyacrylamide	387:400	arg1	SDS-PAGE					423:430	SDS-PAGE	423:430	SDS-PAGE	423:430	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	2	101	theme	polyacrylamide	387:400	arg1	electrophoresis					406:420	Sodium dodecyl sulfate polyacrylamide gel electrophoresis	364:420	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)	364:431	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
32672838	4	102	theme	terminal	1183:1190	arg1	bond					1203:1206	only the terminal glycosidic bond	1174:1206	only the terminal glycosidic bond in the glycoprotein	1174:1226	The presence of other sugars was not detected by 1 H NMR and HPAEC-PAD, suggesting that HPAs hydrolyze only the terminal glycosidic bond in the glycoprotein, resulting in the selective release of sialic acid from AGP.
32672838	2	103	theme	protein	747:753	arg1	core					755:758	the protein core	743:758	the protein core	743:758	Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) show that after incubation of the protein with HPAs at 80 °C and pH 2.8 complete hydrolysis of terminal glycosidic bond has been achieved, resulting in the removal of sialic acids with no observed destruction of the protein core or the residual glycan chains.
33913805	3	0	theme	emended	215:221	arg1	description					223:233	emended description	215:233	emended description	215:233	nov. and emended description of the genus Pseudolysinimonas.
33913805	2	1	theme	larvae	94:99	arg1	gut					87:89	the gut	83:89	the gut of larvae of Protaetia brevitarsis seulensis, reclassification of Lysinimonas yzui as Pseudolysinimonas yzui comb	83:203	nov., isolated from the gut of larvae of Protaetia brevitarsis seulensis, reclassification of Lysinimonas yzui as Pseudolysinimonas yzui comb.
33913805	12	2	dep	iso-C16 	1650:1657	arg1	 0					1659:1660	 0	1659:1660	 0	1659:1660	Those from strain CFWR-9T were type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-11, MK-12 and MK-10, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	12	2	dep	iso-C16 	1650:1657	arg1	anteiso-C15 					1663:1674	anteiso-C15 	1663:1674	anteiso-C15 	1663:1674	Those from strain CFWR-9T were type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-11, MK-12 and MK-10, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	12	2	dep	iso-C16 	1650:1657	arg1	 0					1696:1697	 0	1696:1697	 0	1696:1697	Those from strain CFWR-9T were type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-11, MK-12 and MK-10, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	12	2	dep	iso-C16 	1650:1657	arg1	MK-11					1700:1704	MK-11	1700:1704	MK-11	1700:1704	Those from strain CFWR-9T were type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-11, MK-12 and MK-10, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	12	2	dep	iso-C16 	1650:1657	arg1	diphosphatidylglycerol					1728:1749	diphosphatidylglycerol	1728:1749	diphosphatidylglycerol	1728:1749	Those from strain CFWR-9T were type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-11, MK-12 and MK-10, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	12	2	dep	iso-C16 	1650:1657	arg1	 0					1676:1677	 0	1676:1677	 0	1676:1677	Those from strain CFWR-9T were type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-11, MK-12 and MK-10, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	12	2	dep	iso-C16 	1650:1657	arg1	MK-12					1707:1711	MK-12	1707:1711	MK-12	1707:1711	Those from strain CFWR-9T were type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-11, MK-12 and MK-10, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	10	3	theme	A.	1206:1207	arg1	OAct353T					1222:1229	A. binzhouensis OAct353T	1206:1229	A. binzhouensis OAct353T that was within the radius of the genus Agromyces	1206:1279	The 16S rRNA gene sequence of strain CFWR-9T exhibited the highest similarity (97.7 %) to that of Agromyces binzhouensis OAct353T and the phylogenetic tree indicated that strain CFWR-9T formed one independent cluster with A. binzhouensis OAct353T that was within the radius of the genus Agromyces.
33913805	13	4	theme	novel	1933:1937	arg1	species					1939:1945	a novel species	1931:1945	a novel species	1931:1945	Based on the phenotypic and genotypic data, strains FWR-8T and CFWR-9T each represent a novel species within the genera Protaetiibacter and Agromyces, respectively.
33913805	11	5	theme	type	1406:1409	arg1	type					1300:1303	The peptidoglycan type	1282:1303	The peptidoglycan type	1282:1303	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	5	theme	type	1406:1409	arg1	B1					1411:1412	type B1	1406:1412	type B1	1406:1412	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	5	theme	type	1406:1409	arg1	glycolipids					1556:1566	two unidentified glycolipids	1539:1566	two unidentified glycolipids	1539:1566	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	5	theme	type	1406:1409	arg1	menaquinones					1331:1342	major menaquinones	1325:1342	major menaquinones	1325:1342	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	5	theme	type	1406:1409	arg1	iso-C16 					1415:1422	iso-C16 	1415:1422	iso-C16 	1415:1422	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	5	theme	type	1406:1409	arg1	phosphatidylglycerol					1517:1536	phosphatidylglycerol	1517:1536	phosphatidylglycerol	1517:1536	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	5	theme	type	1406:1409	arg1	acids					1318:1322	major fatty acids	1306:1322	major fatty acids	1306:1322	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	5	theme	type	1406:1409	arg1	 0					1424:1425	 0	1424:1425	 0	1424:1425	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	5	theme	type	1406:1409	arg1	lipid					1589:1593	one unidentified lipid	1572:1593	one unidentified lipid	1572:1593	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	5	theme	type	1406:1409	arg1	lipids					1360:1365	total polar lipids	1348:1365	total polar lipids	1348:1365	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	16	6	theme	19322T=NBRC	2163:2173	arg1	113051T					2175:2181	=KACC 19322T=NBRC 113051T	2157:2181	=KACC 19322T=NBRC 113051T	2157:2181	nov. have been proposed, with the type strains FWR-8T (=KACC 19322T=NBRC 113051T) and CFWR-9T (=KACC 19306T=NBRC 113046T), respectively.
33913805	16	6	theme	19322T=NBRC	2163:2173	arg1	FWR-8T					2149:2154	FWR-8T	2149:2154	FWR-8T	2149:2154	nov. have been proposed, with the type strains FWR-8T (=KACC 19322T=NBRC 113051T) and CFWR-9T (=KACC 19306T=NBRC 113046T), respectively.
33913805	3	7	theme	genus	242:246	arg1	Pseudolysinimonas					248:264	the genus Pseudolysinimonas	238:264	the genus Pseudolysinimonas	238:264	nov. and emended description of the genus Pseudolysinimonas.
33913805	6	8	with	cluster	731:737	arg1	Ptb					744:746	Ptb	744:746	Ptb	744:746	Strain FWR-8T possessed the highest sequence similarity (98.7 %) to that of Protaetiibacter intestinalis 2DFWR-13T and the phylogenetic tree revealed that strain FWR-8T formed a cluster with Ptb.
33913805	2	9	theme	seulensis	126:134	arg1	larvae					94:99	larvae	94:99	larvae of Protaetia brevitarsis seulensis, reclassification of Lysinimonas yzui as Pseudolysinimonas yzui comb	94:203	nov., isolated from the gut of larvae of Protaetia brevitarsis seulensis, reclassification of Lysinimonas yzui as Pseudolysinimonas yzui comb.
33913805	11	10	theme	major	1325:1329	arg1	type					1300:1303	The peptidoglycan type	1282:1303	The peptidoglycan type	1282:1303	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	10	theme	major	1325:1329	arg1	B1					1411:1412	type B1	1406:1412	type B1	1406:1412	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	10	theme	major	1325:1329	arg1	lipids					1360:1365	total polar lipids	1348:1365	total polar lipids	1348:1365	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	10	theme	major	1325:1329	arg1	acids					1318:1322	major fatty acids	1306:1322	major fatty acids	1306:1322	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	10	theme	major	1325:1329	arg1	menaquinones					1331:1342	major menaquinones	1325:1342	major menaquinones	1325:1342	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	6	11	theme	sequence	589:596	arg1	similarity					598:607	the highest sequence similarity	577:607	the highest sequence similarity (98.7 %) to that of Protaetiibacter intestinalis 2DFWR-13T	577:666	Strain FWR-8T possessed the highest sequence similarity (98.7 %) to that of Protaetiibacter intestinalis 2DFWR-13T and the phylogenetic tree revealed that strain FWR-8T formed a cluster with Ptb.
33913805	6	11	theme	sequence	589:596	arg1	%					615:615	98.7 %	610:615	98.7 %	610:615	Strain FWR-8T possessed the highest sequence similarity (98.7 %) to that of Protaetiibacter intestinalis 2DFWR-13T and the phylogenetic tree revealed that strain FWR-8T formed a cluster with Ptb.
33913805	15	12	dep	sp	2098:2099	arg1	Agromyces					2075:2083	Agromyces	2075:2083	Agromyces	2075:2083	nov. and Agromyces intestinalis sp.
33913805	15	12	dep	sp	2098:2099	arg1	nov.					2066:2069	nov.	2066:2069	nov.	2066:2069	nov. and Agromyces intestinalis sp.
33913805	1	13	dep	nov.	27:30	arg1	intestinalis					46:57	intestinalis	46:57	intestinalis	46:57	nov. and Agromyces intestinalis sp.
33913805	11	14	theme	polar	1354:1358	arg1	type					1300:1303	The peptidoglycan type	1282:1303	The peptidoglycan type	1282:1303	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	14	theme	polar	1354:1358	arg1	lipids					1360:1365	total polar lipids	1348:1365	total polar lipids	1348:1365	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	14	theme	polar	1354:1358	arg1	B1					1411:1412	type B1	1406:1412	type B1	1406:1412	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	14	theme	polar	1354:1358	arg1	menaquinones					1331:1342	major menaquinones	1325:1342	major menaquinones	1325:1342	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	14	theme	polar	1354:1358	arg1	acids					1318:1322	major fatty acids	1306:1322	major fatty acids	1306:1322	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	10	15	theme	strain	1014:1019	arg1	CFWR-9T					1021:1027	strain CFWR-9T	1014:1027	strain CFWR-9T	1014:1027	The 16S rRNA gene sequence of strain CFWR-9T exhibited the highest similarity (97.7 %) to that of Agromyces binzhouensis OAct353T and the phylogenetic tree indicated that strain CFWR-9T formed one independent cluster with A. binzhouensis OAct353T that was within the radius of the genus Agromyces.
33913805	15	16	dep	nov.	2066:2069	arg1	intestinalis					2085:2096	intestinalis	2085:2096	intestinalis	2085:2096	nov. and Agromyces intestinalis sp.
33913805	1	17	dep	sp	59:60	arg1	Agromyces					36:44	Agromyces	36:44	Agromyces	36:44	nov. and Agromyces intestinalis sp.
33913805	1	17	dep	sp	59:60	arg1	nov.					27:30	nov.	27:30	nov.	27:30	nov. and Agromyces intestinalis sp.
33913805	16	18	theme	=KACC	2197:2201	arg1	113046T					2215:2221	=KACC 19306T=NBRC 113046T	2197:2221	=KACC 19306T=NBRC 113046T	2197:2221	nov. have been proposed, with the type strains FWR-8T (=KACC 19322T=NBRC 113051T) and CFWR-9T (=KACC 19306T=NBRC 113046T), respectively.
33913805	16	18	theme	=KACC	2197:2201	arg1	CFWR-9T					2188:2194	CFWR-9T	2188:2194	CFWR-9T	2188:2194	nov. have been proposed, with the type strains FWR-8T (=KACC 19322T=NBRC 113051T) and CFWR-9T (=KACC 19306T=NBRC 113046T), respectively.
33913805	8	19	theme	Pseudolysinimonas	773:789	arg1	MSL-13T					802:808	Pseudolysinimonas kribbensis MSL-13T	773:808	Pseudolysinimonas kribbensis MSL-13T	773:808	Pseudolysinimonas kribbensis MSL-13T and Lysinimonas yzui N7XX-4T shared a high 16S rRNA gene sequence similarity (97.8 %) and formed a cluster adjacent to the cluster that included Ptb.
33913805	10	20	theme	highest	1043:1049	arg1	similarity					1051:1060	the highest similarity	1039:1060	the highest similarity (97.7 %) to that of Agromyces binzhouensis OAct353T	1039:1112	The 16S rRNA gene sequence of strain CFWR-9T exhibited the highest similarity (97.7 %) to that of Agromyces binzhouensis OAct353T and the phylogenetic tree indicated that strain CFWR-9T formed one independent cluster with A. binzhouensis OAct353T that was within the radius of the genus Agromyces.
33913805	10	20	theme	highest	1043:1049	arg1	%					1068:1068	97.7 %	1063:1068	97.7 %	1063:1068	The 16S rRNA gene sequence of strain CFWR-9T exhibited the highest similarity (97.7 %) to that of Agromyces binzhouensis OAct353T and the phylogenetic tree indicated that strain CFWR-9T formed one independent cluster with A. binzhouensis OAct353T that was within the radius of the genus Agromyces.
33913805	8	21	theme	16S	853:855	arg1	similarity					876:885	a high 16S rRNA gene sequence similarity	846:885	a high 16S rRNA gene sequence similarity	846:885	Pseudolysinimonas kribbensis MSL-13T and Lysinimonas yzui N7XX-4T shared a high 16S rRNA gene sequence similarity (97.8 %) and formed a cluster adjacent to the cluster that included Ptb.
33913805	6	22	contain	possessed	567:575	arg2	%					615:615	98.7 %	610:615	98.7 %	610:615	Strain FWR-8T possessed the highest sequence similarity (98.7 %) to that of Protaetiibacter intestinalis 2DFWR-13T and the phylogenetic tree revealed that strain FWR-8T formed a cluster with Ptb.
33913805	6	22	contain	possessed	567:575	arg1	FWR-8T					560:565	Strain FWR-8T	553:565	Strain FWR-8T	553:565	Strain FWR-8T possessed the highest sequence similarity (98.7 %) to that of Protaetiibacter intestinalis 2DFWR-13T and the phylogenetic tree revealed that strain FWR-8T formed a cluster with Ptb.
33913805	6	22	contain	possessed	567:575	arg2	similarity					598:607	the highest sequence similarity	577:607	the highest sequence similarity (98.7 %) to that of Protaetiibacter intestinalis 2DFWR-13T	577:666	Strain FWR-8T possessed the highest sequence similarity (98.7 %) to that of Protaetiibacter intestinalis 2DFWR-13T and the phylogenetic tree revealed that strain FWR-8T formed a cluster with Ptb.
33913805	13	23	theme	phenotypic	1858:1867	arg1	data					1883:1886	the phenotypic and genotypic data	1854:1886	the phenotypic and genotypic data	1854:1886	Based on the phenotypic and genotypic data, strains FWR-8T and CFWR-9T each represent a novel species within the genera Protaetiibacter and Agromyces, respectively.
33913805	16	24	dep	strains	2141:2147	arg1	113046T					2215:2221	=KACC 19306T=NBRC 113046T	2197:2221	=KACC 19306T=NBRC 113046T	2197:2221	nov. have been proposed, with the type strains FWR-8T (=KACC 19322T=NBRC 113051T) and CFWR-9T (=KACC 19306T=NBRC 113046T), respectively.
33913805	16	24	dep	strains	2141:2147	arg1	strains					2141:2147	the type strains FWR-8T (=KACC 19322T=NBRC 113051T) and CFWR-9T (=KACC 19306T=NBRC 113046T)	2132:2222	the type strains FWR-8T (=KACC 19322T=NBRC 113051T) and CFWR-9T (=KACC 19306T=NBRC 113046T)	2132:2222	nov. have been proposed, with the type strains FWR-8T (=KACC 19322T=NBRC 113051T) and CFWR-9T (=KACC 19306T=NBRC 113046T), respectively.
33913805	16	24	dep	strains	2141:2147	arg1	113051T					2175:2181	=KACC 19322T=NBRC 113051T	2157:2181	=KACC 19322T=NBRC 113051T	2157:2181	nov. have been proposed, with the type strains FWR-8T (=KACC 19322T=NBRC 113051T) and CFWR-9T (=KACC 19306T=NBRC 113046T), respectively.
33913805	16	24	dep	strains	2141:2147	arg1	CFWR-9T					2188:2194	CFWR-9T	2188:2194	CFWR-9T	2188:2194	nov. have been proposed, with the type strains FWR-8T (=KACC 19322T=NBRC 113051T) and CFWR-9T (=KACC 19306T=NBRC 113046T), respectively.
33913805	16	24	dep	strains	2141:2147	arg1	FWR-8T					2149:2154	FWR-8T	2149:2154	FWR-8T	2149:2154	nov. have been proposed, with the type strains FWR-8T (=KACC 19322T=NBRC 113051T) and CFWR-9T (=KACC 19306T=NBRC 113046T), respectively.
33913805	8	25	theme	gene	862:865	arg1	similarity					876:885	a high 16S rRNA gene sequence similarity	846:885	a high 16S rRNA gene sequence similarity	846:885	Pseudolysinimonas kribbensis MSL-13T and Lysinimonas yzui N7XX-4T shared a high 16S rRNA gene sequence similarity (97.8 %) and formed a cluster adjacent to the cluster that included Ptb.
33913805	4	26	dep	Protaetia	350:358	arg1	seulensis					372:380	Protaetia brevitarsis seulensis	350:380	Protaetia brevitarsis seulensis that were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea	350:478	Two bacterial strains, FWR-8T and CFWR-9T, were isolated from the gut of larvae of Protaetia brevitarsis seulensis that were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
33913805	8	27	theme	Lysinimonas	814:824	arg1	N7XX-4T					831:837	Lysinimonas yzui N7XX-4T	814:837	Lysinimonas yzui N7XX-4T	814:837	Pseudolysinimonas kribbensis MSL-13T and Lysinimonas yzui N7XX-4T shared a high 16S rRNA gene sequence similarity (97.8 %) and formed a cluster adjacent to the cluster that included Ptb.
33913805	10	28	theme	binzhouensis	1092:1103	arg1	OAct353T					1105:1112	Agromyces binzhouensis OAct353T	1082:1112	Agromyces binzhouensis OAct353T	1082:1112	The 16S rRNA gene sequence of strain CFWR-9T exhibited the highest similarity (97.7 %) to that of Agromyces binzhouensis OAct353T and the phylogenetic tree indicated that strain CFWR-9T formed one independent cluster with A. binzhouensis OAct353T that was within the radius of the genus Agromyces.
33913805	12	29	theme	strain	1621:1626	arg1	CFWR-9T					1628:1634	strain CFWR-9T	1621:1634	strain CFWR-9T	1621:1634	Those from strain CFWR-9T were type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-11, MK-12 and MK-10, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	4	30	attach	isolated	315:322	arg2	CFWR-9T					301:307	CFWR-9T	301:307	CFWR-9T	301:307	Two bacterial strains, FWR-8T and CFWR-9T, were isolated from the gut of larvae of Protaetia brevitarsis seulensis that were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
33913805	4	30	attach	isolated	315:322	arg1	gut					333:335	the gut	329:335	the gut of larvae of Protaetia brevitarsis seulensis that were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea	329:478	Two bacterial strains, FWR-8T and CFWR-9T, were isolated from the gut of larvae of Protaetia brevitarsis seulensis that were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
33913805	4	30	attach	isolated	315:322	arg2	FWR-8T					290:295	FWR-8T	290:295	FWR-8T	290:295	Two bacterial strains, FWR-8T and CFWR-9T, were isolated from the gut of larvae of Protaetia brevitarsis seulensis that were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
33913805	4	30	attach	isolated	315:322	arg2	strains					281:287	Two bacterial strains	267:287	Two bacterial strains	267:287	Two bacterial strains, FWR-8T and CFWR-9T, were isolated from the gut of larvae of Protaetia brevitarsis seulensis that were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
33913805	6	31	theme	strain	708:713	arg1	FWR-8T					715:720	strain FWR-8T	708:720	strain FWR-8T	708:720	Strain FWR-8T possessed the highest sequence similarity (98.7 %) to that of Protaetiibacter intestinalis 2DFWR-13T and the phylogenetic tree revealed that strain FWR-8T formed a cluster with Ptb.
33913805	11	32	theme	peptidoglycan	1286:1298	arg1	type					1300:1303	The peptidoglycan type	1282:1303	The peptidoglycan type	1282:1303	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	32	theme	peptidoglycan	1286:1298	arg1	B1					1411:1412	type B1	1406:1412	type B1	1406:1412	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	32	theme	peptidoglycan	1286:1298	arg1	lipids					1360:1365	total polar lipids	1348:1365	total polar lipids	1348:1365	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	32	theme	peptidoglycan	1286:1298	arg1	acids					1318:1322	major fatty acids	1306:1322	major fatty acids	1306:1322	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	32	theme	peptidoglycan	1286:1298	arg1	menaquinones					1331:1342	major menaquinones	1325:1342	major menaquinones	1325:1342	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	10	33	theme	genus	1265:1269	arg1	Agromyces					1271:1279	the genus Agromyces	1261:1279	the genus Agromyces	1261:1279	The 16S rRNA gene sequence of strain CFWR-9T exhibited the highest similarity (97.7 %) to that of Agromyces binzhouensis OAct353T and the phylogenetic tree indicated that strain CFWR-9T formed one independent cluster with A. binzhouensis OAct353T that was within the radius of the genus Agromyces.
33913805	0	34	theme	Protaetiibacter	0:14	arg1	larvae					16:21	Protaetiibacter larvae	0:21	Protaetiibacter larvae	0:21	Protaetiibacter larvae sp.
33913805	10	35	theme	phylogenetic	1122:1133	arg1	tree					1135:1138	the phylogenetic tree	1118:1138	the phylogenetic tree	1118:1138	The 16S rRNA gene sequence of strain CFWR-9T exhibited the highest similarity (97.7 %) to that of Agromyces binzhouensis OAct353T and the phylogenetic tree indicated that strain CFWR-9T formed one independent cluster with A. binzhouensis OAct353T that was within the radius of the genus Agromyces.
33913805	17	36	theme	Pseudolysinimonas	2306:2322	arg1	comb					2329:2332	Pseudolysinimonas yzui comb	2306:2332	Pseudolysinimonas yzui comb	2306:2332	Our results also justify a reclassification of Lysinimonas yzui as Pseudolysinimonas yzui comb.
33913805	17	36	theme	Pseudolysinimonas	2306:2322	arg1	reclassification					2266:2281	a reclassification	2264:2281	a reclassification of Lysinimonas yzui	2264:2301	Our results also justify a reclassification of Lysinimonas yzui as Pseudolysinimonas yzui comb.
33913805	6	37	theme	phylogenetic	676:687	arg1	tree					689:692	the phylogenetic tree	672:692	the phylogenetic tree	672:692	Strain FWR-8T possessed the highest sequence similarity (98.7 %) to that of Protaetiibacter intestinalis 2DFWR-13T and the phylogenetic tree revealed that strain FWR-8T formed a cluster with Ptb.
33913805	2	38	theme	yzui	169:172	arg1	seulensis					126:134	Protaetia brevitarsis seulensis	104:134	Protaetia brevitarsis seulensis	104:134	nov., isolated from the gut of larvae of Protaetia brevitarsis seulensis, reclassification of Lysinimonas yzui as Pseudolysinimonas yzui comb.
33913805	2	38	theme	yzui	169:172	arg1	reclassification					137:152	reclassification	137:152	reclassification of Lysinimonas yzui as Pseudolysinimonas yzui comb	137:203	nov., isolated from the gut of larvae of Protaetia brevitarsis seulensis, reclassification of Lysinimonas yzui as Pseudolysinimonas yzui comb.
33913805	10	39	theme	strain	1155:1160	arg1	CFWR-9T					1162:1168	strain CFWR-9T	1155:1168	strain CFWR-9T	1155:1168	The 16S rRNA gene sequence of strain CFWR-9T exhibited the highest similarity (97.7 %) to that of Agromyces binzhouensis OAct353T and the phylogenetic tree indicated that strain CFWR-9T formed one independent cluster with A. binzhouensis OAct353T that was within the radius of the genus Agromyces.
33913805	14	40	dep	Protaetiibacter	2039:2053	arg1	larvae					2055:2060	Protaetiibacter larvae sp	2039:2063	Protaetiibacter larvae sp	2039:2063	For these species, the names Protaetiibacter larvae sp.
33913805	14	40	dep	Protaetiibacter	2039:2053	arg1	species					2020:2026	these species	2014:2026	these species	2014:2026	For these species, the names Protaetiibacter larvae sp.
33913805	14	40	dep	Protaetiibacter	2039:2053	arg1	names					2033:2037	the names	2029:2037	the names	2029:2037	For these species, the names Protaetiibacter larvae sp.
33913805	14	40	dep	Protaetiibacter	2039:2053	arg1	sp					2062:2063	sp	2062:2063	Protaetiibacter larvae sp	2039:2063	For these species, the names Protaetiibacter larvae sp.
33913805	4	41	theme	larvae	340:345	arg1	gut					333:335	the gut	329:335	the gut of larvae of Protaetia brevitarsis seulensis that were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea	329:478	Two bacterial strains, FWR-8T and CFWR-9T, were isolated from the gut of larvae of Protaetia brevitarsis seulensis that were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
33913805	18	42	theme	Pseudolysinimonas	2380:2396	arg1	nov.					2335:2338	nov.	2335:2338	nov.	2335:2338	nov. and an emended description of the genus Pseudolysinimonas isprovided.
33913805	18	42	theme	Pseudolysinimonas	2380:2396	arg1	description					2355:2365	an emended description	2344:2365	an emended description of the genus Pseudolysinimonas	2344:2396	nov. and an emended description of the genus Pseudolysinimonas isprovided.
33913805	10	43	theme	independent	1181:1191	arg1	cluster					1193:1199	one independent cluster	1177:1199	one independent cluster	1177:1199	The 16S rRNA gene sequence of strain CFWR-9T exhibited the highest similarity (97.7 %) to that of Agromyces binzhouensis OAct353T and the phylogenetic tree indicated that strain CFWR-9T formed one independent cluster with A. binzhouensis OAct353T that was within the radius of the genus Agromyces.
33913805	10	44	theme	gene	997:1000	arg1	sequence					1002:1009	The 16S rRNA gene sequence	984:1009	The 16S rRNA gene sequence of strain CFWR-9T	984:1027	The 16S rRNA gene sequence of strain CFWR-9T exhibited the highest similarity (97.7 %) to that of Agromyces binzhouensis OAct353T and the phylogenetic tree indicated that strain CFWR-9T formed one independent cluster with A. binzhouensis OAct353T that was within the radius of the genus Agromyces.
33913805	11	45	theme	strain	1370:1375	arg1	FWR-8T					1377:1382	strain FWR-8T	1370:1382	strain FWR-8T	1370:1382	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	2	46	theme	Pseudolysinimonas	177:193	arg1	comb					200:203	Pseudolysinimonas yzui comb	177:203	Pseudolysinimonas yzui comb	177:203	nov., isolated from the gut of larvae of Protaetia brevitarsis seulensis, reclassification of Lysinimonas yzui as Pseudolysinimonas yzui comb.
33913805	10	47	theme	16S	988:990	arg1	rRNA					992:995	The 16S rRNA	984:995	The 16S rRNA gene sequence of strain CFWR-9T	984:1027	The 16S rRNA gene sequence of strain CFWR-9T exhibited the highest similarity (97.7 %) to that of Agromyces binzhouensis OAct353T and the phylogenetic tree indicated that strain CFWR-9T formed one independent cluster with A. binzhouensis OAct353T that was within the radius of the genus Agromyces.
33913805	8	48	dep	shared	839:844	arg1	%					893:893	97.8 %	888:893	97.8 %	888:893	Pseudolysinimonas kribbensis MSL-13T and Lysinimonas yzui N7XX-4T shared a high 16S rRNA gene sequence similarity (97.8 %) and formed a cluster adjacent to the cluster that included Ptb.
33913805	16	49	theme	type	2136:2139	arg1	CFWR-9T					2188:2194	CFWR-9T	2188:2194	CFWR-9T	2188:2194	nov. have been proposed, with the type strains FWR-8T (=KACC 19322T=NBRC 113051T) and CFWR-9T (=KACC 19306T=NBRC 113046T), respectively.
33913805	16	49	theme	type	2136:2139	arg1	strains					2141:2147	the type strains FWR-8T (=KACC 19322T=NBRC 113051T) and CFWR-9T (=KACC 19306T=NBRC 113046T)	2132:2222	the type strains FWR-8T (=KACC 19322T=NBRC 113051T) and CFWR-9T (=KACC 19306T=NBRC 113046T)	2132:2222	nov. have been proposed, with the type strains FWR-8T (=KACC 19322T=NBRC 113051T) and CFWR-9T (=KACC 19306T=NBRC 113046T), respectively.
33913805	16	49	theme	type	2136:2139	arg1	FWR-8T					2149:2154	FWR-8T	2149:2154	FWR-8T	2149:2154	nov. have been proposed, with the type strains FWR-8T (=KACC 19322T=NBRC 113051T) and CFWR-9T (=KACC 19306T=NBRC 113046T), respectively.
33913805	17	50	theme	yzui	2298:2301	arg1	comb					2329:2332	Pseudolysinimonas yzui comb	2306:2332	Pseudolysinimonas yzui comb	2306:2332	Our results also justify a reclassification of Lysinimonas yzui as Pseudolysinimonas yzui comb.
33913805	17	50	theme	yzui	2298:2301	arg1	reclassification					2266:2281	a reclassification	2264:2281	a reclassification of Lysinimonas yzui	2264:2301	Our results also justify a reclassification of Lysinimonas yzui as Pseudolysinimonas yzui comb.
33913805	12	51	theme	type	1641:1644	arg1	B1					1646:1647	type B1	1641:1647	type B1	1641:1647	Those from strain CFWR-9T were type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-11, MK-12 and MK-10, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	3	52	theme	Pseudolysinimonas	248:264	arg1	nov.					206:209	nov.	206:209	nov.	206:209	nov. and emended description of the genus Pseudolysinimonas.
33913805	3	52	theme	Pseudolysinimonas	248:264	arg1	description					223:233	emended description	215:233	emended description	215:233	nov. and emended description of the genus Pseudolysinimonas.
33913805	4	53	theme	Protaetia	350:358	arg1	larvae					340:345	larvae	340:345	larvae of Protaetia brevitarsis seulensis that were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea	340:478	Two bacterial strains, FWR-8T and CFWR-9T, were isolated from the gut of larvae of Protaetia brevitarsis seulensis that were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
33913805	13	54	theme	genotypic	1873:1881	arg1	data					1883:1886	the phenotypic and genotypic data	1854:1886	the phenotypic and genotypic data	1854:1886	Based on the phenotypic and genotypic data, strains FWR-8T and CFWR-9T each represent a novel species within the genera Protaetiibacter and Agromyces, respectively.
33913805	11	55	theme	fatty	1312:1316	arg1	type					1300:1303	The peptidoglycan type	1282:1303	The peptidoglycan type	1282:1303	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	55	theme	fatty	1312:1316	arg1	B1					1411:1412	type B1	1406:1412	type B1	1406:1412	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	55	theme	fatty	1312:1316	arg1	lipids					1360:1365	total polar lipids	1348:1365	total polar lipids	1348:1365	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	55	theme	fatty	1312:1316	arg1	acids					1318:1322	major fatty acids	1306:1322	major fatty acids	1306:1322	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	55	theme	fatty	1312:1316	arg1	menaquinones					1331:1342	major menaquinones	1325:1342	major menaquinones	1325:1342	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	6	56	theme	highest	581:587	arg1	similarity					598:607	the highest sequence similarity	577:607	the highest sequence similarity (98.7 %) to that of Protaetiibacter intestinalis 2DFWR-13T	577:666	Strain FWR-8T possessed the highest sequence similarity (98.7 %) to that of Protaetiibacter intestinalis 2DFWR-13T and the phylogenetic tree revealed that strain FWR-8T formed a cluster with Ptb.
33913805	6	56	theme	highest	581:587	arg1	%					615:615	98.7 %	610:615	98.7 %	610:615	Strain FWR-8T possessed the highest sequence similarity (98.7 %) to that of Protaetiibacter intestinalis 2DFWR-13T and the phylogenetic tree revealed that strain FWR-8T formed a cluster with Ptb.
33913805	10	57	theme	CFWR-9T	1021:1027	arg1	sequence					1002:1009	The 16S rRNA gene sequence	984:1009	The 16S rRNA gene sequence of strain CFWR-9T	984:1027	The 16S rRNA gene sequence of strain CFWR-9T exhibited the highest similarity (97.7 %) to that of Agromyces binzhouensis OAct353T and the phylogenetic tree indicated that strain CFWR-9T formed one independent cluster with A. binzhouensis OAct353T that was within the radius of the genus Agromyces.
33913805	4	58	theme	National	406:413	arg1	Institute					415:423	the National Institute	402:423	the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea	402:478	Two bacterial strains, FWR-8T and CFWR-9T, were isolated from the gut of larvae of Protaetia brevitarsis seulensis that were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
33913805	16	59	theme	19306T=NBRC	2203:2213	arg1	113046T					2215:2221	=KACC 19306T=NBRC 113046T	2197:2221	=KACC 19306T=NBRC 113046T	2197:2221	nov. have been proposed, with the type strains FWR-8T (=KACC 19322T=NBRC 113051T) and CFWR-9T (=KACC 19306T=NBRC 113046T), respectively.
33913805	16	59	theme	19306T=NBRC	2203:2213	arg1	CFWR-9T					2188:2194	CFWR-9T	2188:2194	CFWR-9T	2188:2194	nov. have been proposed, with the type strains FWR-8T (=KACC 19322T=NBRC 113051T) and CFWR-9T (=KACC 19306T=NBRC 113046T), respectively.
33913805	6	60	theme	Protaetiibacter	629:643	arg1	intestinalis					645:656	Protaetiibacter intestinalis 2DFWR-13T	629:666	Protaetiibacter intestinalis 2DFWR-13T	629:666	Strain FWR-8T possessed the highest sequence similarity (98.7 %) to that of Protaetiibacter intestinalis 2DFWR-13T and the phylogenetic tree revealed that strain FWR-8T formed a cluster with Ptb.
33913805	8	61	theme	high	848:851	arg1	similarity					876:885	a high 16S rRNA gene sequence similarity	846:885	a high 16S rRNA gene sequence similarity	846:885	Pseudolysinimonas kribbensis MSL-13T and Lysinimonas yzui N7XX-4T shared a high 16S rRNA gene sequence similarity (97.8 %) and formed a cluster adjacent to the cluster that included Ptb.
33913805	13	62	dep	strains	1889:1895	arg1	FWR-8T					1897:1902	FWR-8T	1897:1902	FWR-8T	1897:1902	Based on the phenotypic and genotypic data, strains FWR-8T and CFWR-9T each represent a novel species within the genera Protaetiibacter and Agromyces, respectively.
33913805	13	62	dep	strains	1889:1895	arg1	each					1916:1919	each	1916:1919	each	1916:1919	Based on the phenotypic and genotypic data, strains FWR-8T and CFWR-9T each represent a novel species within the genera Protaetiibacter and Agromyces, respectively.
33913805	13	62	dep	strains	1889:1895	arg1	strains					1889:1895	strains FWR-8T and CFWR-9T	1889:1914	strains FWR-8T and CFWR-9T each	1889:1919	Based on the phenotypic and genotypic data, strains FWR-8T and CFWR-9T each represent a novel species within the genera Protaetiibacter and Agromyces, respectively.
33913805	13	62	dep	strains	1889:1895	arg1	CFWR-9T					1908:1914	CFWR-9T	1908:1914	CFWR-9T	1908:1914	Based on the phenotypic and genotypic data, strains FWR-8T and CFWR-9T each represent a novel species within the genera Protaetiibacter and Agromyces, respectively.
33913805	8	63	theme	kribbensis	791:800	arg1	MSL-13T					802:808	Pseudolysinimonas kribbensis MSL-13T	773:808	Pseudolysinimonas kribbensis MSL-13T	773:808	Pseudolysinimonas kribbensis MSL-13T and Lysinimonas yzui N7XX-4T shared a high 16S rRNA gene sequence similarity (97.8 %) and formed a cluster adjacent to the cluster that included Ptb.
33913805	8	64	theme	rRNA	857:860	arg1	similarity					876:885	a high 16S rRNA gene sequence similarity	846:885	a high 16S rRNA gene sequence similarity	846:885	Pseudolysinimonas kribbensis MSL-13T and Lysinimonas yzui N7XX-4T shared a high 16S rRNA gene sequence similarity (97.8 %) and formed a cluster adjacent to the cluster that included Ptb.
33913805	0	65	dep	sp	23:24	arg1	larvae					16:21	Protaetiibacter larvae	0:21	Protaetiibacter larvae	0:21	Protaetiibacter larvae sp.
33913805	8	66	theme	sequence	867:874	arg1	similarity					876:885	a high 16S rRNA gene sequence similarity	846:885	a high 16S rRNA gene sequence similarity	846:885	Pseudolysinimonas kribbensis MSL-13T and Lysinimonas yzui N7XX-4T shared a high 16S rRNA gene sequence similarity (97.8 %) and formed a cluster adjacent to the cluster that included Ptb.
33913805	4	67	theme	Republic	462:469	arg1	Institute					415:423	the National Institute	402:423	the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea	402:478	Two bacterial strains, FWR-8T and CFWR-9T, were isolated from the gut of larvae of Protaetia brevitarsis seulensis that were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
33913805	16	68	theme	=KACC	2157:2161	arg1	113051T					2175:2181	=KACC 19322T=NBRC 113051T	2157:2181	=KACC 19322T=NBRC 113051T	2157:2181	nov. have been proposed, with the type strains FWR-8T (=KACC 19322T=NBRC 113051T) and CFWR-9T (=KACC 19306T=NBRC 113046T), respectively.
33913805	16	68	theme	=KACC	2157:2161	arg1	FWR-8T					2149:2154	FWR-8T	2149:2154	FWR-8T	2149:2154	nov. have been proposed, with the type strains FWR-8T (=KACC 19322T=NBRC 113051T) and CFWR-9T (=KACC 19306T=NBRC 113046T), respectively.
33913805	8	69	theme	yzui	826:829	arg1	N7XX-4T					831:837	Lysinimonas yzui N7XX-4T	814:837	Lysinimonas yzui N7XX-4T	814:837	Pseudolysinimonas kribbensis MSL-13T and Lysinimonas yzui N7XX-4T shared a high 16S rRNA gene sequence similarity (97.8 %) and formed a cluster adjacent to the cluster that included Ptb.
33913805	10	70	theme	Agromyces	1082:1090	arg1	OAct353T					1105:1112	Agromyces binzhouensis OAct353T	1082:1112	Agromyces binzhouensis OAct353T	1082:1112	The 16S rRNA gene sequence of strain CFWR-9T exhibited the highest similarity (97.7 %) to that of Agromyces binzhouensis OAct353T and the phylogenetic tree indicated that strain CFWR-9T formed one independent cluster with A. binzhouensis OAct353T that was within the radius of the genus Agromyces.
33913805	13	71	dep	genera	1958:1963	arg1	Agromyces					1985:1993	Agromyces	1985:1993	Agromyces	1985:1993	Based on the phenotypic and genotypic data, strains FWR-8T and CFWR-9T each represent a novel species within the genera Protaetiibacter and Agromyces, respectively.
33913805	13	71	dep	genera	1958:1963	arg1	Protaetiibacter					1965:1979	Protaetiibacter	1965:1979	Protaetiibacter	1965:1979	Based on the phenotypic and genotypic data, strains FWR-8T and CFWR-9T each represent a novel species within the genera Protaetiibacter and Agromyces, respectively.
33913805	4	72	theme	Korea	474:478	arg1	Institute					415:423	the National Institute	402:423	the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea	402:478	Two bacterial strains, FWR-8T and CFWR-9T, were isolated from the gut of larvae of Protaetia brevitarsis seulensis that were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
33913805	11	73	theme	total	1348:1352	arg1	type					1300:1303	The peptidoglycan type	1282:1303	The peptidoglycan type	1282:1303	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	73	theme	total	1348:1352	arg1	lipids					1360:1365	total polar lipids	1348:1365	total polar lipids	1348:1365	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	73	theme	total	1348:1352	arg1	B1					1411:1412	type B1	1406:1412	type B1	1406:1412	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	73	theme	total	1348:1352	arg1	menaquinones					1331:1342	major menaquinones	1325:1342	major menaquinones	1325:1342	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	73	theme	total	1348:1352	arg1	acids					1318:1322	major fatty acids	1306:1322	major fatty acids	1306:1322	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	10	74	theme	Agromyces	1271:1279	arg1	radius					1251:1256	the radius	1247:1256	the radius of the genus Agromyces	1247:1279	The 16S rRNA gene sequence of strain CFWR-9T exhibited the highest similarity (97.7 %) to that of Agromyces binzhouensis OAct353T and the phylogenetic tree indicated that strain CFWR-9T formed one independent cluster with A. binzhouensis OAct353T that was within the radius of the genus Agromyces.
33913805	6	75	theme	Strain	553:558	arg1	FWR-8T					560:565	Strain FWR-8T	553:565	Strain FWR-8T	553:565	Strain FWR-8T possessed the highest sequence similarity (98.7 %) to that of Protaetiibacter intestinalis 2DFWR-13T and the phylogenetic tree revealed that strain FWR-8T formed a cluster with Ptb.
33913805	17	76	theme	yzui	2324:2327	arg1	comb					2329:2332	Pseudolysinimonas yzui comb	2306:2332	Pseudolysinimonas yzui comb	2306:2332	Our results also justify a reclassification of Lysinimonas yzui as Pseudolysinimonas yzui comb.
33913805	17	76	theme	yzui	2324:2327	arg1	reclassification					2266:2281	a reclassification	2264:2281	a reclassification of Lysinimonas yzui	2264:2301	Our results also justify a reclassification of Lysinimonas yzui as Pseudolysinimonas yzui comb.
33913805	12	77	theme	unidentified	1811:1822	arg1	lipid					1824:1828	one unidentified lipid	1807:1828	one unidentified lipid	1807:1828	Those from strain CFWR-9T were type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-11, MK-12 and MK-10, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	4	78	theme	bacterial	271:279	arg1	FWR-8T					290:295	FWR-8T	290:295	FWR-8T	290:295	Two bacterial strains, FWR-8T and CFWR-9T, were isolated from the gut of larvae of Protaetia brevitarsis seulensis that were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
33913805	4	78	theme	bacterial	271:279	arg1	strains					281:287	Two bacterial strains	267:287	Two bacterial strains	267:287	Two bacterial strains, FWR-8T and CFWR-9T, were isolated from the gut of larvae of Protaetia brevitarsis seulensis that were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
33913805	4	78	theme	bacterial	271:279	arg1	CFWR-9T					301:307	CFWR-9T	301:307	CFWR-9T	301:307	Two bacterial strains, FWR-8T and CFWR-9T, were isolated from the gut of larvae of Protaetia brevitarsis seulensis that were raised at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
33913805	18	79	theme	emended	2347:2353	arg1	description					2355:2365	an emended description	2344:2365	an emended description of the genus Pseudolysinimonas	2344:2396	nov. and an emended description of the genus Pseudolysinimonas isprovided.
33913805	2	80	theme	Lysinimonas	157:167	arg1	yzui					169:172	Lysinimonas yzui	157:172	Lysinimonas yzui as Pseudolysinimonas yzui comb	157:203	nov., isolated from the gut of larvae of Protaetia brevitarsis seulensis, reclassification of Lysinimonas yzui as Pseudolysinimonas yzui comb.
33913805	11	81	theme	unidentified	1543:1554	arg1	B1					1411:1412	type B1	1406:1412	type B1	1406:1412	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	81	theme	unidentified	1543:1554	arg1	glycolipids					1556:1566	two unidentified glycolipids	1539:1566	two unidentified glycolipids	1539:1566	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	10	82	dep	A.	1206:1207	arg1	binzhouensis					1209:1220	binzhouensis	1209:1220	binzhouensis	1209:1220	The 16S rRNA gene sequence of strain CFWR-9T exhibited the highest similarity (97.7 %) to that of Agromyces binzhouensis OAct353T and the phylogenetic tree indicated that strain CFWR-9T formed one independent cluster with A. binzhouensis OAct353T that was within the radius of the genus Agromyces.
33913805	8	83	theme	adjacent	917:924	arg1	cluster					909:915	a cluster	907:915	a cluster adjacent to the cluster that included Ptb	907:957	Pseudolysinimonas kribbensis MSL-13T and Lysinimonas yzui N7XX-4T shared a high 16S rRNA gene sequence similarity (97.8 %) and formed a cluster adjacent to the cluster that included Ptb.
33913805	11	84	theme	major	1306:1310	arg1	type					1300:1303	The peptidoglycan type	1282:1303	The peptidoglycan type	1282:1303	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	84	theme	major	1306:1310	arg1	B1					1411:1412	type B1	1406:1412	type B1	1406:1412	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	84	theme	major	1306:1310	arg1	lipids					1360:1365	total polar lipids	1348:1365	total polar lipids	1348:1365	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	84	theme	major	1306:1310	arg1	acids					1318:1322	major fatty acids	1306:1322	major fatty acids	1306:1322	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	84	theme	major	1306:1310	arg1	menaquinones					1331:1342	major menaquinones	1325:1342	major menaquinones	1325:1342	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	12	85	theme	unidentified	1778:1789	arg1	glycolipids					1791:1801	two unidentified glycolipids	1774:1801	two unidentified glycolipids	1774:1801	Those from strain CFWR-9T were type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-11, MK-12 and MK-10, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	18	86	theme	genus	2374:2378	arg1	Pseudolysinimonas					2380:2396	the genus Pseudolysinimonas	2370:2396	the genus Pseudolysinimonas	2370:2396	nov. and an emended description of the genus Pseudolysinimonas isprovided.
33913805	2	87	theme	yzui	195:198	arg1	comb					200:203	Pseudolysinimonas yzui comb	177:203	Pseudolysinimonas yzui comb	177:203	nov., isolated from the gut of larvae of Protaetia brevitarsis seulensis, reclassification of Lysinimonas yzui as Pseudolysinimonas yzui comb.
33913805	11	88	theme	unidentified	1576:1587	arg1	B1					1411:1412	type B1	1406:1412	type B1	1406:1412	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	88	theme	unidentified	1576:1587	arg1	lipid					1589:1593	one unidentified lipid	1572:1593	one unidentified lipid	1572:1593	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	10	89	theme	rRNA	992:995	arg1	sequence					1002:1009	The 16S rRNA gene sequence	984:1009	The 16S rRNA gene sequence of strain CFWR-9T	984:1027	The 16S rRNA gene sequence of strain CFWR-9T exhibited the highest similarity (97.7 %) to that of Agromyces binzhouensis OAct353T and the phylogenetic tree indicated that strain CFWR-9T formed one independent cluster with A. binzhouensis OAct353T that was within the radius of the genus Agromyces.
33913805	11	90	theme	FWR-8T	1377:1382	arg1	B1					1411:1412	type B1	1406:1412	type B1	1406:1412	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	90	theme	FWR-8T	1377:1382	arg1	type					1300:1303	The peptidoglycan type	1282:1303	The peptidoglycan type	1282:1303	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	90	theme	FWR-8T	1377:1382	arg1	lipids					1360:1365	total polar lipids	1348:1365	total polar lipids	1348:1365	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	90	theme	FWR-8T	1377:1382	arg1	acids					1318:1322	major fatty acids	1306:1322	major fatty acids	1306:1322	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
33913805	11	90	theme	FWR-8T	1377:1382	arg1	menaquinones					1331:1342	major menaquinones	1325:1342	major menaquinones	1325:1342	The peptidoglycan type, major fatty acids, major menaquinones and total polar lipids of strain FWR-8T were characterized as type B1, iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0, MK-15, MK-16 and MK-14, and diphosphatidylglycerol, phosphatidylglycerol, two unidentified glycolipids and one unidentified lipid, respectively.
35237590	0	0	theme	Effect	103:108	arg1	Evaluation					85:94	Evaluation	85:94	Evaluation of Its Effect on Modulating Intestinal Microbiota in High-Fat-Diet-Fed Mice	85:170	Enzymatic Preparation of Low-Molecular-Weight Laminaria japonica Polysaccharides and Evaluation of Its Effect on Modulating Intestinal Microbiota in High-Fat-Diet-Fed Mice.
35237590	0	0	theme	Effect	103:108	arg1	Preparation					10:20	Enzymatic Preparation	0:20	Enzymatic Preparation of Low-Molecular-Weight Laminaria japonica Polysaccharides	0:79	Enzymatic Preparation of Low-Molecular-Weight Laminaria japonica Polysaccharides and Evaluation of Its Effect on Modulating Intestinal Microbiota in High-Fat-Diet-Fed Mice.
35237590	7	1	theme	gut	1264:1266	arg1	dysbiosis					1279:1287	HFD-induced gut microbiota dysbiosis	1252:1287	HFD-induced gut microbiota dysbiosis	1252:1287	SJP ameliorated HFD-induced gut microbiota dysbiosis, resulting in increased abundance of Faecalibaculum, Romboutsia, and Clostridium sensu stricto 1 and decreased abundance of Blautia and Lactobacillus.
35237590	2	2	theme	seaweed	385:391	arg1	polysaccharides					393:407	seaweed polysaccharides	385:407	seaweed polysaccharides	385:407	However, kelp, a natural source of seaweed polysaccharides, is highly viscous, making it difficult to prepare dietary fiber by simple degradation.
35237590	6	3	theme	weight	1083:1088	arg1	gain					1090:1093	weight gain	1083:1093	weight gain	1083:1093	SJP reduced weight gain and fat deposition in HFD-fed mice and increased the concentration of total SCFAs, including acetate, propionate, and butyrate in the feces.
35237590	7	4	theme	Lactobacillus	1425:1437	arg1	abundance					1400:1408	decreased abundance	1390:1408	decreased abundance of Blautia and Lactobacillus	1390:1437	SJP ameliorated HFD-induced gut microbiota dysbiosis, resulting in increased abundance of Faecalibaculum, Romboutsia, and Clostridium sensu stricto 1 and decreased abundance of Blautia and Lactobacillus.
35237590	7	4	theme	Lactobacillus	1425:1437	arg1	abundance					1313:1321	increased abundance	1303:1321	increased abundance of Faecalibaculum, Romboutsia, and Clostridium sensu stricto 1	1303:1384	SJP ameliorated HFD-induced gut microbiota dysbiosis, resulting in increased abundance of Faecalibaculum, Romboutsia, and Clostridium sensu stricto 1 and decreased abundance of Blautia and Lactobacillus.
35237590	8	5	theme	muciniphila	1491:1501	arg1	abundance					1466:1474	the abundance	1462:1474	the abundance of Akkermansia muciniphila in mice provided with HFD and normal chow	1462:1543	Further, SJP enhanced the abundance of Akkermansia muciniphila in mice provided with HFD and normal chow.
35237590	5	6	theme	in	967:968	arg1	effects					975:981	The in vivo effects	963:981	The in vivo effects of SJP on the intestinal microbiota	963:1017	The in vivo effects of SJP on the intestinal microbiota were also investigated using HFD-fed C57BL/6J mice.
35237590	4	7	used	utilized	813:820	arg2	SJP					795:797	SJP	795:797	SJP	795:797	Seaweed L. japonica polysaccharides (SJP) were rapidly utilized by the human fecal microbiota in vitro, resulting in the generation of short-chain fatty acids (SCFAs), specifically acetate and propionate.
35237590	4	7	used	utilized	813:820	arg2	polysaccharides					778:792	Seaweed L. japonica polysaccharides	758:792	Seaweed L. japonica polysaccharides (SJP)	758:798	Seaweed L. japonica polysaccharides (SJP) were rapidly utilized by the human fecal microbiota in vitro, resulting in the generation of short-chain fatty acids (SCFAs), specifically acetate and propionate.
35237590	6	8	from	gain	1090:1093	arg1	mice					1125:1128	HFD-fed mice	1117:1128	HFD-fed mice	1117:1128	SJP reduced weight gain and fat deposition in HFD-fed mice and increased the concentration of total SCFAs, including acetate, propionate, and butyrate in the feces.
35237590	4	9	theme	L.	766:767	arg1	polysaccharides					778:792	Seaweed L. japonica polysaccharides	758:792	Seaweed L. japonica polysaccharides (SJP)	758:798	Seaweed L. japonica polysaccharides (SJP) were rapidly utilized by the human fecal microbiota in vitro, resulting in the generation of short-chain fatty acids (SCFAs), specifically acetate and propionate.
35237590	4	9	theme	L.	766:767	arg1	SJP					795:797	SJP	795:797	SJP	795:797	Seaweed L. japonica polysaccharides (SJP) were rapidly utilized by the human fecal microbiota in vitro, resulting in the generation of short-chain fatty acids (SCFAs), specifically acetate and propionate.
35237590	3	10	from	Laminaria	591:599	arg1	polysaccharides					570:584	low-molecular-weight polysaccharides	549:584	low-molecular-weight polysaccharides from Laminaria japonica	549:608	Therefore, we developed a novel method of preparing low-molecular-weight polysaccharides from Laminaria japonica by combining high-pressure pretreatment and composite enzymatic degradation and evaluated the obesity prevention activity of these polysaccharides.
35237590	7	11	theme	HFD-induced	1252:1262	arg1	dysbiosis					1279:1287	HFD-induced gut microbiota dysbiosis	1252:1287	HFD-induced gut microbiota dysbiosis	1252:1287	SJP ameliorated HFD-induced gut microbiota dysbiosis, resulting in increased abundance of Faecalibaculum, Romboutsia, and Clostridium sensu stricto 1 and decreased abundance of Blautia and Lactobacillus.
35237590	4	12	theme	fecal	835:839	arg1	microbiota					841:850	the human fecal microbiota	825:850	the human fecal microbiota	825:850	Seaweed L. japonica polysaccharides (SJP) were rapidly utilized by the human fecal microbiota in vitro, resulting in the generation of short-chain fatty acids (SCFAs), specifically acetate and propionate.
35237590	10	13	theme	composite	1705:1713	arg1	degradation					1725:1735	composite enzymatic degradation	1705:1735	composite enzymatic degradation (cellulase and recombinant alginate lyase)	1705:1778	This study highlights the potential use of SJP, prepared using composite enzymatic degradation (cellulase and recombinant alginate lyase), in preventing obesity and restoring intestinal homeostasis in obese individuals.
35237590	3	14	theme	novel	523:527	arg1	method					529:534	a novel method	521:534	a novel method of preparing low-molecular-weight polysaccharides from Laminaria japonica by combining high-pressure pretreatment and composite enzymatic degradation	521:684	Therefore, we developed a novel method of preparing low-molecular-weight polysaccharides from Laminaria japonica by combining high-pressure pretreatment and composite enzymatic degradation and evaluated the obesity prevention activity of these polysaccharides.
35237590	8	15	from	abundance	1466:1474	arg1	mice					1506:1509	mice	1506:1509	mice provided with HFD and normal chow	1506:1543	Further, SJP enhanced the abundance of Akkermansia muciniphila in mice provided with HFD and normal chow.
35237590	1	16	theme	regulatory	311:320	arg1	function					322:329	the regulatory function	307:329	the regulatory function of gut microbiota	307:347	Recent studies have shown that seaweed polysaccharides can ameliorate high-fat-diet (HFD)-induced metabolic syndromes associated with the regulatory function of gut microbiota.
35237590	7	17	theme	sensu	1370:1374	arg1	stricto					1376:1382	Clostridium sensu stricto 1	1358:1384	Clostridium sensu stricto 1	1358:1384	SJP ameliorated HFD-induced gut microbiota dysbiosis, resulting in increased abundance of Faecalibaculum, Romboutsia, and Clostridium sensu stricto 1 and decreased abundance of Blautia and Lactobacillus.
35237590	3	18	theme	low-molecular-weight	549:568	arg1	polysaccharides					570:584	low-molecular-weight polysaccharides	549:584	low-molecular-weight polysaccharides from Laminaria japonica	549:608	Therefore, we developed a novel method of preparing low-molecular-weight polysaccharides from Laminaria japonica by combining high-pressure pretreatment and composite enzymatic degradation and evaluated the obesity prevention activity of these polysaccharides.
35237590	7	19	theme	stricto	1376:1382	arg1	abundance					1400:1408	decreased abundance	1390:1408	decreased abundance of Blautia and Lactobacillus	1390:1437	SJP ameliorated HFD-induced gut microbiota dysbiosis, resulting in increased abundance of Faecalibaculum, Romboutsia, and Clostridium sensu stricto 1 and decreased abundance of Blautia and Lactobacillus.
35237590	7	19	theme	stricto	1376:1382	arg1	abundance					1313:1321	increased abundance	1303:1321	increased abundance of Faecalibaculum, Romboutsia, and Clostridium sensu stricto 1	1303:1384	SJP ameliorated HFD-induced gut microbiota dysbiosis, resulting in increased abundance of Faecalibaculum, Romboutsia, and Clostridium sensu stricto 1 and decreased abundance of Blautia and Lactobacillus.
35237590	0	20	theme	Intestinal	124:133	arg1	Microbiota					135:144	Intestinal Microbiota	124:144	Intestinal Microbiota	124:144	Enzymatic Preparation of Low-Molecular-Weight Laminaria japonica Polysaccharides and Evaluation of Its Effect on Modulating Intestinal Microbiota in High-Fat-Diet-Fed Mice.
35237590	4	21	theme	acids	911:915	arg1	generation					879:888	the generation	875:888	the generation of short-chain fatty acids (SCFAs), specifically acetate and propionate	875:960	Seaweed L. japonica polysaccharides (SJP) were rapidly utilized by the human fecal microbiota in vitro, resulting in the generation of short-chain fatty acids (SCFAs), specifically acetate and propionate.
35237590	4	22	dep	L.	766:767	arg1	japonica					769:776	japonica	769:776	japonica	769:776	Seaweed L. japonica polysaccharides (SJP) were rapidly utilized by the human fecal microbiota in vitro, resulting in the generation of short-chain fatty acids (SCFAs), specifically acetate and propionate.
35237590	1	23	theme	seaweed	204:210	arg1	polysaccharides					212:226	seaweed polysaccharides	204:226	seaweed polysaccharides	204:226	Recent studies have shown that seaweed polysaccharides can ameliorate high-fat-diet (HFD)-induced metabolic syndromes associated with the regulatory function of gut microbiota.
35237590	7	24	theme	microbiota	1268:1277	arg1	dysbiosis					1279:1287	HFD-induced gut microbiota dysbiosis	1252:1287	HFD-induced gut microbiota dysbiosis	1252:1287	SJP ameliorated HFD-induced gut microbiota dysbiosis, resulting in increased abundance of Faecalibaculum, Romboutsia, and Clostridium sensu stricto 1 and decreased abundance of Blautia and Lactobacillus.
35237590	10	25	theme	potential	1668:1676	arg1	use					1678:1680	the potential use	1664:1680	the potential use of SJP, prepared using composite enzymatic degradation (cellulase and recombinant alginate lyase), in preventing obesity and restoring intestinal homeostasis in obese individuals	1664:1859	This study highlights the potential use of SJP, prepared using composite enzymatic degradation (cellulase and recombinant alginate lyase), in preventing obesity and restoring intestinal homeostasis in obese individuals.
35237590	9	26	theme	muciniphila	1629:1639	arg1	growth					1616:1621	the growth	1612:1621	the growth of A. muciniphila	1612:1639	Single-strain culture experiments also revealed that SJP promoted the growth of A. muciniphila.
35237590	7	27	theme	Faecalibaculum	1326:1339	arg1	abundance					1400:1408	decreased abundance	1390:1408	decreased abundance of Blautia and Lactobacillus	1390:1437	SJP ameliorated HFD-induced gut microbiota dysbiosis, resulting in increased abundance of Faecalibaculum, Romboutsia, and Clostridium sensu stricto 1 and decreased abundance of Blautia and Lactobacillus.
35237590	7	27	theme	Faecalibaculum	1326:1339	arg1	abundance					1313:1321	increased abundance	1303:1321	increased abundance of Faecalibaculum, Romboutsia, and Clostridium sensu stricto 1	1303:1384	SJP ameliorated HFD-induced gut microbiota dysbiosis, resulting in increased abundance of Faecalibaculum, Romboutsia, and Clostridium sensu stricto 1 and decreased abundance of Blautia and Lactobacillus.
35237590	4	28	theme	Seaweed	758:764	arg1	polysaccharides					778:792	Seaweed L. japonica polysaccharides	758:792	Seaweed L. japonica polysaccharides (SJP)	758:798	Seaweed L. japonica polysaccharides (SJP) were rapidly utilized by the human fecal microbiota in vitro, resulting in the generation of short-chain fatty acids (SCFAs), specifically acetate and propionate.
35237590	4	28	theme	Seaweed	758:764	arg1	SJP					795:797	SJP	795:797	SJP	795:797	Seaweed L. japonica polysaccharides (SJP) were rapidly utilized by the human fecal microbiota in vitro, resulting in the generation of short-chain fatty acids (SCFAs), specifically acetate and propionate.
35237590	0	29	theme	Enzymatic	0:8	arg1	Preparation					10:20	Enzymatic Preparation	0:20	Enzymatic Preparation of Low-Molecular-Weight Laminaria japonica Polysaccharides	0:79	Enzymatic Preparation of Low-Molecular-Weight Laminaria japonica Polysaccharides and Evaluation of Its Effect on Modulating Intestinal Microbiota in High-Fat-Diet-Fed Mice.
35237590	10	30	theme	alginate	1764:1771	arg1	lyase					1773:1777	recombinant alginate lyase	1752:1777	recombinant alginate lyase	1752:1777	This study highlights the potential use of SJP, prepared using composite enzymatic degradation (cellulase and recombinant alginate lyase), in preventing obesity and restoring intestinal homeostasis in obese individuals.
35237590	3	31	theme	high-pressure	623:635	arg1	pretreatment					637:648	high-pressure pretreatment	623:648	high-pressure pretreatment	623:648	Therefore, we developed a novel method of preparing low-molecular-weight polysaccharides from Laminaria japonica by combining high-pressure pretreatment and composite enzymatic degradation and evaluated the obesity prevention activity of these polysaccharides.
35237590	6	32	theme	HFD-fed	1117:1123	arg1	mice					1125:1128	HFD-fed mice	1117:1128	HFD-fed mice	1117:1128	SJP reduced weight gain and fat deposition in HFD-fed mice and increased the concentration of total SCFAs, including acetate, propionate, and butyrate in the feces.
35237590	2	33	theme	dietary	460:466	arg1	fiber					468:472	dietary fiber	460:472	dietary fiber	460:472	However, kelp, a natural source of seaweed polysaccharides, is highly viscous, making it difficult to prepare dietary fiber by simple degradation.
35237590	0	34	theme	High-Fat-Diet-Fed	149:165	arg1	Mice					167:170	High-Fat-Diet-Fed Mice	149:170	High-Fat-Diet-Fed Mice	149:170	Enzymatic Preparation of Low-Molecular-Weight Laminaria japonica Polysaccharides and Evaluation of Its Effect on Modulating Intestinal Microbiota in High-Fat-Diet-Fed Mice.
35237590	5	35	theme	SJP	986:988	arg1	effects					975:981	The in vivo effects	963:981	The in vivo effects of SJP on the intestinal microbiota	963:1017	The in vivo effects of SJP on the intestinal microbiota were also investigated using HFD-fed C57BL/6J mice.
35237590	4	36	theme	human	829:833	arg1	microbiota					841:850	the human fecal microbiota	825:850	the human fecal microbiota	825:850	Seaweed L. japonica polysaccharides (SJP) were rapidly utilized by the human fecal microbiota in vitro, resulting in the generation of short-chain fatty acids (SCFAs), specifically acetate and propionate.
35237590	7	37	theme	Clostridium	1358:1368	arg1	stricto					1376:1382	Clostridium sensu stricto 1	1358:1384	Clostridium sensu stricto 1	1358:1384	SJP ameliorated HFD-induced gut microbiota dysbiosis, resulting in increased abundance of Faecalibaculum, Romboutsia, and Clostridium sensu stricto 1 and decreased abundance of Blautia and Lactobacillus.
35237590	5	38	dep	in	967:968	arg1	vivo					970:973	vivo	970:973	vivo	970:973	The in vivo effects of SJP on the intestinal microbiota were also investigated using HFD-fed C57BL/6J mice.
35237590	1	39	theme	gut	334:336	arg1	microbiota					338:347	gut microbiota	334:347	gut microbiota	334:347	Recent studies have shown that seaweed polysaccharides can ameliorate high-fat-diet (HFD)-induced metabolic syndromes associated with the regulatory function of gut microbiota.
35237590	10	40	theme	intestinal	1817:1826	arg1	homeostasis					1828:1838	intestinal homeostasis	1817:1838	intestinal homeostasis in obese individuals	1817:1859	This study highlights the potential use of SJP, prepared using composite enzymatic degradation (cellulase and recombinant alginate lyase), in preventing obesity and restoring intestinal homeostasis in obese individuals.
35237590	10	41	theme	obese	1843:1847	arg1	individuals					1849:1859	obese individuals	1843:1859	obese individuals	1843:1859	This study highlights the potential use of SJP, prepared using composite enzymatic degradation (cellulase and recombinant alginate lyase), in preventing obesity and restoring intestinal homeostasis in obese individuals.
35237590	6	42	theme	total	1165:1169	arg1	butyrate					1213:1220	butyrate	1213:1220	butyrate	1213:1220	SJP reduced weight gain and fat deposition in HFD-fed mice and increased the concentration of total SCFAs, including acetate, propionate, and butyrate in the feces.
35237590	6	42	theme	total	1165:1169	arg1	propionate					1197:1206	propionate	1197:1206	propionate	1197:1206	SJP reduced weight gain and fat deposition in HFD-fed mice and increased the concentration of total SCFAs, including acetate, propionate, and butyrate in the feces.
35237590	6	42	theme	total	1165:1169	arg1	SCFAs					1171:1175	total SCFAs	1165:1175	total SCFAs	1165:1175	SJP reduced weight gain and fat deposition in HFD-fed mice and increased the concentration of total SCFAs, including acetate, propionate, and butyrate in the feces.
35237590	6	42	theme	total	1165:1169	arg1	acetate					1188:1194	acetate	1188:1194	acetate	1188:1194	SJP reduced weight gain and fat deposition in HFD-fed mice and increased the concentration of total SCFAs, including acetate, propionate, and butyrate in the feces.
35237590	0	43	theme	japonica	56:63	arg1	Polysaccharides					65:79	Low-Molecular-Weight Laminaria japonica Polysaccharides	25:79	Low-Molecular-Weight Laminaria japonica Polysaccharides	25:79	Enzymatic Preparation of Low-Molecular-Weight Laminaria japonica Polysaccharides and Evaluation of Its Effect on Modulating Intestinal Microbiota in High-Fat-Diet-Fed Mice.
35237590	1	44	theme	microbiota	338:347	arg1	function					322:329	the regulatory function	307:329	the regulatory function of gut microbiota	307:347	Recent studies have shown that seaweed polysaccharides can ameliorate high-fat-diet (HFD)-induced metabolic syndromes associated with the regulatory function of gut microbiota.
35237590	9	45	theme	culture	1560:1566	arg1	experiments					1568:1578	Single-strain culture experiments	1546:1578	Single-strain culture experiments	1546:1578	Single-strain culture experiments also revealed that SJP promoted the growth of A. muciniphila.
35237590	7	46	theme	increased	1303:1311	arg1	abundance					1313:1321	increased abundance	1303:1321	increased abundance of Faecalibaculum, Romboutsia, and Clostridium sensu stricto 1	1303:1384	SJP ameliorated HFD-induced gut microbiota dysbiosis, resulting in increased abundance of Faecalibaculum, Romboutsia, and Clostridium sensu stricto 1 and decreased abundance of Blautia and Lactobacillus.
35237590	3	47	theme	composite	654:662	arg1	degradation					674:684	composite enzymatic degradation	654:684	composite enzymatic degradation	654:684	Therefore, we developed a novel method of preparing low-molecular-weight polysaccharides from Laminaria japonica by combining high-pressure pretreatment and composite enzymatic degradation and evaluated the obesity prevention activity of these polysaccharides.
35237590	10	48	from	homeostasis	1828:1838	arg1	individuals					1849:1859	obese individuals	1843:1859	obese individuals	1843:1859	This study highlights the potential use of SJP, prepared using composite enzymatic degradation (cellulase and recombinant alginate lyase), in preventing obesity and restoring intestinal homeostasis in obese individuals.
35237590	6	49	theme	fat	1099:1101	arg1	deposition					1103:1112	fat deposition	1099:1112	fat deposition in HFD-fed mice	1099:1128	SJP reduced weight gain and fat deposition in HFD-fed mice and increased the concentration of total SCFAs, including acetate, propionate, and butyrate in the feces.
35237590	0	50	theme	Laminaria	46:54	arg1	Polysaccharides					65:79	Low-Molecular-Weight Laminaria japonica Polysaccharides	25:79	Low-Molecular-Weight Laminaria japonica Polysaccharides	25:79	Enzymatic Preparation of Low-Molecular-Weight Laminaria japonica Polysaccharides and Evaluation of Its Effect on Modulating Intestinal Microbiota in High-Fat-Diet-Fed Mice.
35237590	3	51	theme	obesity	704:710	arg1	activity					723:730	the obesity prevention activity	700:730	the obesity prevention activity of these polysaccharides	700:755	Therefore, we developed a novel method of preparing low-molecular-weight polysaccharides from Laminaria japonica by combining high-pressure pretreatment and composite enzymatic degradation and evaluated the obesity prevention activity of these polysaccharides.
35237590	0	52	theme	Low-Molecular-Weight	25:44	arg1	Polysaccharides					65:79	Low-Molecular-Weight Laminaria japonica Polysaccharides	25:79	Low-Molecular-Weight Laminaria japonica Polysaccharides	25:79	Enzymatic Preparation of Low-Molecular-Weight Laminaria japonica Polysaccharides and Evaluation of Its Effect on Modulating Intestinal Microbiota in High-Fat-Diet-Fed Mice.
35237590	8	53	theme	normal	1533:1538	arg1	chow					1540:1543	normal chow	1533:1543	normal chow	1533:1543	Further, SJP enhanced the abundance of Akkermansia muciniphila in mice provided with HFD and normal chow.
35237590	3	54	theme	prevention	712:721	arg1	activity					723:730	the obesity prevention activity	700:730	the obesity prevention activity of these polysaccharides	700:755	Therefore, we developed a novel method of preparing low-molecular-weight polysaccharides from Laminaria japonica by combining high-pressure pretreatment and composite enzymatic degradation and evaluated the obesity prevention activity of these polysaccharides.
35237590	4	55	theme	fatty	905:909	arg1	SCFAs					918:922	SCFAs	918:922	SCFAs	918:922	Seaweed L. japonica polysaccharides (SJP) were rapidly utilized by the human fecal microbiota in vitro, resulting in the generation of short-chain fatty acids (SCFAs), specifically acetate and propionate.
35237590	4	55	theme	fatty	905:909	arg1	acids					911:915	short-chain fatty acids	893:915	short-chain fatty acids (SCFAs)	893:923	Seaweed L. japonica polysaccharides (SJP) were rapidly utilized by the human fecal microbiota in vitro, resulting in the generation of short-chain fatty acids (SCFAs), specifically acetate and propionate.
35237590	4	55	theme	fatty	905:909	arg1	acetate					939:945	acetate	939:945	acetate	939:945	Seaweed L. japonica polysaccharides (SJP) were rapidly utilized by the human fecal microbiota in vitro, resulting in the generation of short-chain fatty acids (SCFAs), specifically acetate and propionate.
35237590	4	55	theme	fatty	905:909	arg1	propionate					951:960	propionate	951:960	propionate	951:960	Seaweed L. japonica polysaccharides (SJP) were rapidly utilized by the human fecal microbiota in vitro, resulting in the generation of short-chain fatty acids (SCFAs), specifically acetate and propionate.
35237590	9	56	theme	Single-strain	1546:1558	arg1	experiments					1568:1578	Single-strain culture experiments	1546:1578	Single-strain culture experiments	1546:1578	Single-strain culture experiments also revealed that SJP promoted the growth of A. muciniphila.
35237590	0	57	theme	Polysaccharides	65:79	arg1	Evaluation					85:94	Evaluation	85:94	Evaluation of Its Effect on Modulating Intestinal Microbiota in High-Fat-Diet-Fed Mice	85:170	Enzymatic Preparation of Low-Molecular-Weight Laminaria japonica Polysaccharides and Evaluation of Its Effect on Modulating Intestinal Microbiota in High-Fat-Diet-Fed Mice.
35237590	0	57	theme	Polysaccharides	65:79	arg1	Preparation					10:20	Enzymatic Preparation	0:20	Enzymatic Preparation of Low-Molecular-Weight Laminaria japonica Polysaccharides	0:79	Enzymatic Preparation of Low-Molecular-Weight Laminaria japonica Polysaccharides and Evaluation of Its Effect on Modulating Intestinal Microbiota in High-Fat-Diet-Fed Mice.
35237590	2	58	theme	simple	477:482	arg1	degradation					484:494	simple degradation	477:494	simple degradation	477:494	However, kelp, a natural source of seaweed polysaccharides, is highly viscous, making it difficult to prepare dietary fiber by simple degradation.
35237590	4	59	theme	short-chain	893:903	arg1	SCFAs					918:922	SCFAs	918:922	SCFAs	918:922	Seaweed L. japonica polysaccharides (SJP) were rapidly utilized by the human fecal microbiota in vitro, resulting in the generation of short-chain fatty acids (SCFAs), specifically acetate and propionate.
35237590	4	59	theme	short-chain	893:903	arg1	acids					911:915	short-chain fatty acids	893:915	short-chain fatty acids (SCFAs)	893:923	Seaweed L. japonica polysaccharides (SJP) were rapidly utilized by the human fecal microbiota in vitro, resulting in the generation of short-chain fatty acids (SCFAs), specifically acetate and propionate.
35237590	4	59	theme	short-chain	893:903	arg1	acetate					939:945	acetate	939:945	acetate	939:945	Seaweed L. japonica polysaccharides (SJP) were rapidly utilized by the human fecal microbiota in vitro, resulting in the generation of short-chain fatty acids (SCFAs), specifically acetate and propionate.
35237590	4	59	theme	short-chain	893:903	arg1	propionate					951:960	propionate	951:960	propionate	951:960	Seaweed L. japonica polysaccharides (SJP) were rapidly utilized by the human fecal microbiota in vitro, resulting in the generation of short-chain fatty acids (SCFAs), specifically acetate and propionate.
35237590	2	60	theme	natural	367:373	arg1	source					375:380	a natural source	365:380	a natural source of seaweed polysaccharides	365:407	However, kelp, a natural source of seaweed polysaccharides, is highly viscous, making it difficult to prepare dietary fiber by simple degradation.
35237590	2	60	theme	natural	367:373	arg1	kelp					359:362	kelp	359:362	kelp	359:362	However, kelp, a natural source of seaweed polysaccharides, is highly viscous, making it difficult to prepare dietary fiber by simple degradation.
35237590	8	61	theme	Akkermansia	1479:1489	arg1	muciniphila					1491:1501	Akkermansia muciniphila	1479:1501	Akkermansia muciniphila	1479:1501	Further, SJP enhanced the abundance of Akkermansia muciniphila in mice provided with HFD and normal chow.
35237590	5	62	from	effects	975:981	arg1	microbiota					1008:1017	the intestinal microbiota	993:1017	the intestinal microbiota	993:1017	The in vivo effects of SJP on the intestinal microbiota were also investigated using HFD-fed C57BL/6J mice.
35237590	7	63	theme	Blautia	1413:1419	arg1	abundance					1400:1408	decreased abundance	1390:1408	decreased abundance of Blautia and Lactobacillus	1390:1437	SJP ameliorated HFD-induced gut microbiota dysbiosis, resulting in increased abundance of Faecalibaculum, Romboutsia, and Clostridium sensu stricto 1 and decreased abundance of Blautia and Lactobacillus.
35237590	7	63	theme	Blautia	1413:1419	arg1	abundance					1313:1321	increased abundance	1303:1321	increased abundance of Faecalibaculum, Romboutsia, and Clostridium sensu stricto 1	1303:1384	SJP ameliorated HFD-induced gut microbiota dysbiosis, resulting in increased abundance of Faecalibaculum, Romboutsia, and Clostridium sensu stricto 1 and decreased abundance of Blautia and Lactobacillus.
35237590	5	64	theme	HFD-fed	1048:1054	arg1	mice					1065:1068	HFD-fed C57BL/6J mice	1048:1068	HFD-fed C57BL/6J mice	1048:1068	The in vivo effects of SJP on the intestinal microbiota were also investigated using HFD-fed C57BL/6J mice.
35237590	3	65	theme	enzymatic	664:672	arg1	degradation					674:684	composite enzymatic degradation	654:684	composite enzymatic degradation	654:684	Therefore, we developed a novel method of preparing low-molecular-weight polysaccharides from Laminaria japonica by combining high-pressure pretreatment and composite enzymatic degradation and evaluated the obesity prevention activity of these polysaccharides.
35237590	10	66	theme	enzymatic	1715:1723	arg1	degradation					1725:1735	composite enzymatic degradation	1705:1735	composite enzymatic degradation (cellulase and recombinant alginate lyase)	1705:1778	This study highlights the potential use of SJP, prepared using composite enzymatic degradation (cellulase and recombinant alginate lyase), in preventing obesity and restoring intestinal homeostasis in obese individuals.
35237590	5	67	theme	intestinal	997:1006	arg1	microbiota					1008:1017	the intestinal microbiota	993:1017	the intestinal microbiota	993:1017	The in vivo effects of SJP on the intestinal microbiota were also investigated using HFD-fed C57BL/6J mice.
35237590	9	68	theme	A.	1626:1627	arg1	muciniphila					1629:1639	A. muciniphila	1626:1639	A. muciniphila	1626:1639	Single-strain culture experiments also revealed that SJP promoted the growth of A. muciniphila.
35237590	7	69	theme	Romboutsia	1342:1351	arg1	abundance					1400:1408	decreased abundance	1390:1408	decreased abundance of Blautia and Lactobacillus	1390:1437	SJP ameliorated HFD-induced gut microbiota dysbiosis, resulting in increased abundance of Faecalibaculum, Romboutsia, and Clostridium sensu stricto 1 and decreased abundance of Blautia and Lactobacillus.
35237590	7	69	theme	Romboutsia	1342:1351	arg1	abundance					1313:1321	increased abundance	1303:1321	increased abundance of Faecalibaculum, Romboutsia, and Clostridium sensu stricto 1	1303:1384	SJP ameliorated HFD-induced gut microbiota dysbiosis, resulting in increased abundance of Faecalibaculum, Romboutsia, and Clostridium sensu stricto 1 and decreased abundance of Blautia and Lactobacillus.
35237590	5	70	theme	C57BL/6J	1056:1063	arg1	mice					1065:1068	HFD-fed C57BL/6J mice	1048:1068	HFD-fed C57BL/6J mice	1048:1068	The in vivo effects of SJP on the intestinal microbiota were also investigated using HFD-fed C57BL/6J mice.
35237590	6	71	from	concentration	1148:1160	arg1	feces					1229:1233	the feces	1225:1233	the feces	1225:1233	SJP reduced weight gain and fat deposition in HFD-fed mice and increased the concentration of total SCFAs, including acetate, propionate, and butyrate in the feces.
35237590	3	72	dep	Laminaria	591:599	arg1	japonica					601:608	Laminaria japonica	591:608	Laminaria japonica	591:608	Therefore, we developed a novel method of preparing low-molecular-weight polysaccharides from Laminaria japonica by combining high-pressure pretreatment and composite enzymatic degradation and evaluated the obesity prevention activity of these polysaccharides.
35237590	1	73	theme	-induced	262:269	arg1	syndromes					281:289	high-fat-diet (HFD)-induced metabolic syndromes	243:289	high-fat-diet (HFD)-induced metabolic syndromes associated with the regulatory function of gut microbiota	243:347	Recent studies have shown that seaweed polysaccharides can ameliorate high-fat-diet (HFD)-induced metabolic syndromes associated with the regulatory function of gut microbiota.
35237590	3	74	theme	polysaccharides	741:755	arg1	activity					723:730	the obesity prevention activity	700:730	the obesity prevention activity of these polysaccharides	700:755	Therefore, we developed a novel method of preparing low-molecular-weight polysaccharides from Laminaria japonica by combining high-pressure pretreatment and composite enzymatic degradation and evaluated the obesity prevention activity of these polysaccharides.
35237590	10	75	dep	degradation	1725:1735	arg1	cellulase					1738:1746	cellulase	1738:1746	cellulase	1738:1746	This study highlights the potential use of SJP, prepared using composite enzymatic degradation (cellulase and recombinant alginate lyase), in preventing obesity and restoring intestinal homeostasis in obese individuals.
35237590	10	75	dep	degradation	1725:1735	arg1	lyase					1773:1777	recombinant alginate lyase	1752:1777	recombinant alginate lyase	1752:1777	This study highlights the potential use of SJP, prepared using composite enzymatic degradation (cellulase and recombinant alginate lyase), in preventing obesity and restoring intestinal homeostasis in obese individuals.
35237590	10	76	theme	recombinant	1752:1762	arg1	lyase					1773:1777	recombinant alginate lyase	1752:1777	recombinant alginate lyase	1752:1777	This study highlights the potential use of SJP, prepared using composite enzymatic degradation (cellulase and recombinant alginate lyase), in preventing obesity and restoring intestinal homeostasis in obese individuals.
35237590	1	77	theme	Recent	173:178	arg1	studies					180:186	Recent studies	173:186	Recent studies	173:186	Recent studies have shown that seaweed polysaccharides can ameliorate high-fat-diet (HFD)-induced metabolic syndromes associated with the regulatory function of gut microbiota.
35237590	7	78	theme	decreased	1390:1398	arg1	abundance					1400:1408	decreased abundance	1390:1408	decreased abundance of Blautia and Lactobacillus	1390:1437	SJP ameliorated HFD-induced gut microbiota dysbiosis, resulting in increased abundance of Faecalibaculum, Romboutsia, and Clostridium sensu stricto 1 and decreased abundance of Blautia and Lactobacillus.
35237590	1	79	theme	metabolic	271:279	arg1	syndromes					281:289	high-fat-diet (HFD)-induced metabolic syndromes	243:289	high-fat-diet (HFD)-induced metabolic syndromes associated with the regulatory function of gut microbiota	243:347	Recent studies have shown that seaweed polysaccharides can ameliorate high-fat-diet (HFD)-induced metabolic syndromes associated with the regulatory function of gut microbiota.
35237590	10	80	theme	SJP	1685:1687	arg1	use					1678:1680	the potential use	1664:1680	the potential use of SJP, prepared using composite enzymatic degradation (cellulase and recombinant alginate lyase), in preventing obesity and restoring intestinal homeostasis in obese individuals	1664:1859	This study highlights the potential use of SJP, prepared using composite enzymatic degradation (cellulase and recombinant alginate lyase), in preventing obesity and restoring intestinal homeostasis in obese individuals.
35237590	6	81	from	deposition	1103:1112	arg1	mice					1125:1128	HFD-fed mice	1117:1128	HFD-fed mice	1117:1128	SJP reduced weight gain and fat deposition in HFD-fed mice and increased the concentration of total SCFAs, including acetate, propionate, and butyrate in the feces.
35237590	6	82	theme	SCFAs	1171:1175	arg1	concentration					1148:1160	the concentration	1144:1160	the concentration of total SCFAs, including acetate, propionate, and butyrate in the feces	1144:1233	SJP reduced weight gain and fat deposition in HFD-fed mice and increased the concentration of total SCFAs, including acetate, propionate, and butyrate in the feces.
35237590	2	83	theme	polysaccharides	393:407	arg1	source					375:380	a natural source	365:380	a natural source of seaweed polysaccharides	365:407	However, kelp, a natural source of seaweed polysaccharides, is highly viscous, making it difficult to prepare dietary fiber by simple degradation.
35237590	2	83	theme	polysaccharides	393:407	arg1	kelp					359:362	kelp	359:362	kelp	359:362	However, kelp, a natural source of seaweed polysaccharides, is highly viscous, making it difficult to prepare dietary fiber by simple degradation.
32375935	3	0	theme	irregular	487:495	arg1	clusters					497:504	irregular clusters	487:504	irregular clusters	487:504	The organism was aerobic, and cells were non-spore-forming, non-motile cocci that occurred singly, in pairs, in triplets, in tetrads, in short chains or in irregular clusters.
32375935	1	1	attach	isolated	88:95	arg2	Dermacoccaceae					73:86	the family Dermacoccaceae	62:86	the family Dermacoccaceae isolated from a cave soil	62:112	nov., a new member of the family Dermacoccaceae isolated from a cave soil.
32375935	1	1	attach	isolated	88:95	arg1	soil					109:112	a cave soil	102:112	a cave soil	102:112	nov., a new member of the family Dermacoccaceae isolated from a cave soil.
32375935	12	2	theme	Luteipulveratus	1279:1293	arg1	radiation					1256:1264	the radiation	1252:1264	the radiation of the genus Luteipulveratus	1252:1293	Phylogenetic analyses based on 16S rRNA gene sequences showed that the novel isolate belonged to the family Dermacoccaceae and formed a distinct subcluster at the base of the radiation of the genus Luteipulveratus.
32375935	15	3	dep	data	1661:1664	arg1	the					1648:1650	the	1648:1650	the	1648:1650	On the basis of data from this polyphasic study, strain C5-26T (=KCTC 39632T=DSM 108676T) represents a novel species of a new genus in the family Dermacoccaceae, for which the name Leekyejoonella antrihumi gen. nov., sp.
32375935	15	3	dep	data	1661:1664	arg1	basis					1652:1656	basis	1652:1656	basis	1652:1656	On the basis of data from this polyphasic study, strain C5-26T (=KCTC 39632T=DSM 108676T) represents a novel species of a new genus in the family Dermacoccaceae, for which the name Leekyejoonella antrihumi gen. nov., sp.
32375935	12	4	theme	genus	1273:1277	arg1	Luteipulveratus					1279:1293	the genus Luteipulveratus	1269:1293	the genus Luteipulveratus	1269:1293	Phylogenetic analyses based on 16S rRNA gene sequences showed that the novel isolate belonged to the family Dermacoccaceae and formed a distinct subcluster at the base of the radiation of the genus Luteipulveratus.
32375935	8	5	contain	contained	788:796	arg2	diphosphatidylglycerol					798:819	diphosphatidylglycerol	798:819	diphosphatidylglycerol	798:819	The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid.
32375935	8	5	contain	contained	788:796	arg1	lipids					781:786	The polar lipids	771:786	The polar lipids	771:786	The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid.
32375935	8	5	contain	contained	788:796	arg2	phosphatidylinositol					844:863	phosphatidylinositol	844:863	phosphatidylinositol	844:863	The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid.
32375935	8	5	contain	contained	788:796	arg2	phospholipid					885:896	an unidentified phospholipid	869:896	an unidentified phospholipid	869:896	The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid.
32375935	8	5	contain	contained	788:796	arg2	phosphatidylglycerol					822:841	phosphatidylglycerol	822:841	phosphatidylglycerol	822:841	The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid.
32375935	11	6	theme	genomic	1054:1060	arg1	DNA					1062:1064	the genomic DNA	1050:1064	the genomic DNA	1050:1064	The G+C content of the genomic DNA was 67.1 mol%.
32375935	2	7	dep	Gram-stain-positive	123:141	arg1	actinobacterial					144:158	actinobacterial	144:158	actinobacterial	144:158	A novel Gram-stain-positive, actinobacterial strain, designated C5-26T, was isolated from soil from a natural cave in Jeju, Republic of Korea, and its taxonomic position was investigated using a polyphasic approach.
32375935	5	8	theme	interpeptide	621:632	arg1	bridge					634:639	an l-Ser-d-Asp interpeptide bridge	606:639	an l-Ser-d-Asp interpeptide bridge	606:639	The peptidoglycan type was A4α with an l-Ser-d-Asp interpeptide bridge.
32375935	15	9	from	genus	1771:1775	arg1	Dermacoccaceae					1791:1804	the family Dermacoccaceae	1780:1804	the family Dermacoccaceae	1780:1804	On the basis of data from this polyphasic study, strain C5-26T (=KCTC 39632T=DSM 108676T) represents a novel species of a new genus in the family Dermacoccaceae, for which the name Leekyejoonella antrihumi gen. nov., sp.
32375935	15	10	theme	novel	1748:1752	arg1	species					1754:1760	a novel species	1746:1760	a novel species	1746:1760	On the basis of data from this polyphasic study, strain C5-26T (=KCTC 39632T=DSM 108676T) represents a novel species of a new genus in the family Dermacoccaceae, for which the name Leekyejoonella antrihumi gen. nov., sp.
32375935	0	11	dep	sp	36:37	arg1	nov.					30:33	Leekyejoonella antrihumi gen. nov.	0:33	Leekyejoonella antrihumi gen. nov.	0:33	Leekyejoonella antrihumi gen. nov., sp.
32375935	11	12	theme	DNA	1062:1064	arg1	%					1078:1078	67.1 mol%	1070:1078	67.1 mol%	1070:1078	The G+C content of the genomic DNA was 67.1 mol%.
32375935	11	12	theme	DNA	1062:1064	arg1	content					1039:1045	The G+C content	1031:1045	The G+C content of the genomic DNA	1031:1064	The G+C content of the genomic DNA was 67.1 mol%.
32375935	2	13	theme	novel	117:121	arg1	strain					160:165	A novel Gram-stain-positive, actinobacterial strain	115:165	A novel Gram-stain-positive, actinobacterial strain	115:165	A novel Gram-stain-positive, actinobacterial strain, designated C5-26T, was isolated from soil from a natural cave in Jeju, Republic of Korea, and its taxonomic position was investigated using a polyphasic approach.
32375935	13	14	theme	hedensis	1433:1440	arg1	strains					1370:1376	the type strains	1361:1376	the type strains of Luteipulveratus halotolerans (96.2 %), Branchiibius hedensis (95.4 %), Luteipulveratus mongoliensis (95.4 %) and Branchiibius cervicis (95.3 %)	1361:1523	Highest sequence similarities of the novel isolate were found to the type strains of Luteipulveratus halotolerans (96.2 %), Branchiibius hedensis (95.4 %), Luteipulveratus mongoliensis (95.4 %) and Branchiibius cervicis (95.3 %).
32375935	12	15	theme	Phylogenetic	1081:1092	arg1	analyses					1094:1101	Phylogenetic analyses	1081:1101	Phylogenetic analyses based on 16S rRNA gene sequences	1081:1134	Phylogenetic analyses based on 16S rRNA gene sequences showed that the novel isolate belonged to the family Dermacoccaceae and formed a distinct subcluster at the base of the radiation of the genus Luteipulveratus.
32375935	15	16	theme	Leekyejoonella	1826:1839	arg1	nov.					1856:1859	the name Leekyejoonella antrihumi gen. nov.	1817:1859	the name Leekyejoonella antrihumi gen. nov.	1817:1859	On the basis of data from this polyphasic study, strain C5-26T (=KCTC 39632T=DSM 108676T) represents a novel species of a new genus in the family Dermacoccaceae, for which the name Leekyejoonella antrihumi gen. nov., sp.
32375935	2	17	attach	isolated	191:198	arg1	soil					205:208	soil	205:208	soil	205:208	A novel Gram-stain-positive, actinobacterial strain, designated C5-26T, was isolated from soil from a natural cave in Jeju, Republic of Korea, and its taxonomic position was investigated using a polyphasic approach.
32375935	2	17	attach	isolated	191:198	arg2	strain					160:165	A novel Gram-stain-positive, actinobacterial strain	115:165	A novel Gram-stain-positive, actinobacterial strain	115:165	A novel Gram-stain-positive, actinobacterial strain, designated C5-26T, was isolated from soil from a natural cave in Jeju, Republic of Korea, and its taxonomic position was investigated using a polyphasic approach.
32375935	2	17	attach	isolated	191:198	arg1	cave					225:228	a natural cave	215:228	a natural cave in Jeju, Republic of Korea	215:255	A novel Gram-stain-positive, actinobacterial strain, designated C5-26T, was isolated from soil from a natural cave in Jeju, Republic of Korea, and its taxonomic position was investigated using a polyphasic approach.
32375935	13	18	theme	cervicis	1507:1514	arg1	strains					1370:1376	the type strains	1361:1376	the type strains of Luteipulveratus halotolerans (96.2 %), Branchiibius hedensis (95.4 %), Luteipulveratus mongoliensis (95.4 %) and Branchiibius cervicis (95.3 %)	1361:1523	Highest sequence similarities of the novel isolate were found to the type strains of Luteipulveratus halotolerans (96.2 %), Branchiibius hedensis (95.4 %), Luteipulveratus mongoliensis (95.4 %) and Branchiibius cervicis (95.3 %).
32375935	13	19	theme	novel	1333:1337	arg1	isolate					1339:1345	the novel isolate	1329:1345	the novel isolate	1329:1345	Highest sequence similarities of the novel isolate were found to the type strains of Luteipulveratus halotolerans (96.2 %), Branchiibius hedensis (95.4 %), Luteipulveratus mongoliensis (95.4 %) and Branchiibius cervicis (95.3 %).
32375935	1	20	theme	new	48:50	arg1	member					52:57	a new member	46:57	a new member of the family Dermacoccaceae isolated from a cave soil	46:112	nov., a new member of the family Dermacoccaceae isolated from a cave soil.
32375935	1	20	theme	new	48:50	arg1	nov.					40:43	nov.	40:43	nov.	40:43	nov., a new member of the family Dermacoccaceae isolated from a cave soil.
32375935	3	21	theme	non-spore-forming	372:388	arg1	cocci					402:406	non-spore-forming, non-motile cocci	372:406	non-spore-forming, non-motile cocci that occurred singly, in pairs, in triplets, in tetrads, in short chains or in irregular clusters	372:504	The organism was aerobic, and cells were non-spore-forming, non-motile cocci that occurred singly, in pairs, in triplets, in tetrads, in short chains or in irregular clusters.
32375935	14	22	theme	genome-based	1536:1547	arg1	phylogeny					1549:1557	The whole genome-based phylogeny	1526:1557	The whole genome-based phylogeny	1526:1557	The whole genome-based phylogeny supported the novelty of the isolate at the genus level in the family Dermacoccaceae.
32375935	14	23	theme	whole	1530:1534	arg1	phylogeny					1549:1557	The whole genome-based phylogeny	1526:1557	The whole genome-based phylogeny	1526:1557	The whole genome-based phylogeny supported the novelty of the isolate at the genus level in the family Dermacoccaceae.
32375935	9	24	dep	iso-C16 	926:933	arg1	 1 h					951:954	 1 h	951:954	iso-C16 : 0 and iso-C16 : 1 h	926:954	The major fatty acids were iso-C16 : 0 and iso-C16 : 1 h.
32375935	9	24	dep	iso-C16 	926:933	arg1	iso-C16 					942:949	iso-C16 	942:949	iso-C16 	942:949	The major fatty acids were iso-C16 : 0 and iso-C16 : 1 h.
32375935	9	24	dep	iso-C16 	926:933	arg1	 0					935:936	 0	935:936	 0	935:936	The major fatty acids were iso-C16 : 0 and iso-C16 : 1 h.
32375935	15	25	from	species	1754:1760	arg1	Dermacoccaceae					1791:1804	the family Dermacoccaceae	1780:1804	the family Dermacoccaceae	1780:1804	On the basis of data from this polyphasic study, strain C5-26T (=KCTC 39632T=DSM 108676T) represents a novel species of a new genus in the family Dermacoccaceae, for which the name Leekyejoonella antrihumi gen. nov., sp.
32375935	12	26	theme	radiation	1256:1264	arg1	base					1244:1247	the base	1240:1247	the base of the radiation of the genus Luteipulveratus	1240:1293	Phylogenetic analyses based on 16S rRNA gene sequences showed that the novel isolate belonged to the family Dermacoccaceae and formed a distinct subcluster at the base of the radiation of the genus Luteipulveratus.
32375935	7	27	theme	major	739:743	arg1	menaquinone					745:755	The major menaquinone	735:755	The major menaquinone	735:755	The major menaquinone was MK-8(H4).
32375935	7	27	theme	major	739:743	arg1	MK-8					761:764	MK-8	761:764	MK-8(H4)	761:768	The major menaquinone was MK-8(H4).
32375935	0	28	theme	antrihumi	15:23	arg1	nov.					30:33	Leekyejoonella antrihumi gen. nov.	0:33	Leekyejoonella antrihumi gen. nov.	0:33	Leekyejoonella antrihumi gen. nov., sp.
32375935	10	29	theme	genome	979:984	arg1	size					961:964	The size	957:964	The size of the draft genome	957:984	The size of the draft genome was 5.32 Mbp with depth of coverage of 161×.
32375935	10	29	theme	genome	979:984	arg1	Mbp					995:997	5.32 Mbp	990:997	5.32 Mbp	990:997	The size of the draft genome was 5.32 Mbp with depth of coverage of 161×.
32375935	15	30	theme	name	1821:1824	arg1	nov.					1856:1859	the name Leekyejoonella antrihumi gen. nov.	1817:1859	the name Leekyejoonella antrihumi gen. nov.	1817:1859	On the basis of data from this polyphasic study, strain C5-26T (=KCTC 39632T=DSM 108676T) represents a novel species of a new genus in the family Dermacoccaceae, for which the name Leekyejoonella antrihumi gen. nov., sp.
32375935	12	31	theme	16S	1112:1114	arg1	sequences					1126:1134	16S rRNA gene sequences	1112:1134	16S rRNA gene sequences	1112:1134	Phylogenetic analyses based on 16S rRNA gene sequences showed that the novel isolate belonged to the family Dermacoccaceae and formed a distinct subcluster at the base of the radiation of the genus Luteipulveratus.
32375935	13	32	theme	halotolerans	1397:1408	arg1	strains					1370:1376	the type strains	1361:1376	the type strains of Luteipulveratus halotolerans (96.2 %), Branchiibius hedensis (95.4 %), Luteipulveratus mongoliensis (95.4 %) and Branchiibius cervicis (95.3 %)	1361:1523	Highest sequence similarities of the novel isolate were found to the type strains of Luteipulveratus halotolerans (96.2 %), Branchiibius hedensis (95.4 %), Luteipulveratus mongoliensis (95.4 %) and Branchiibius cervicis (95.3 %).
32375935	0	33	theme	Leekyejoonella	0:13	arg1	nov.					30:33	Leekyejoonella antrihumi gen. nov.	0:33	Leekyejoonella antrihumi gen. nov.	0:33	Leekyejoonella antrihumi gen. nov., sp.
32375935	10	34	theme	coverage	1013:1020	arg1	depth					1004:1008	depth	1004:1008	depth of coverage of 161×	1004:1028	The size of the draft genome was 5.32 Mbp with depth of coverage of 161×.
32375935	11	35	theme	67.1 mol	1070:1077	arg1	%					1078:1078	67.1 mol%	1070:1078	67.1 mol%	1070:1078	The G+C content of the genomic DNA was 67.1 mol%.
32375935	11	35	theme	67.1 mol	1070:1077	arg1	content					1039:1045	The G+C content	1031:1045	The G+C content of the genomic DNA	1031:1064	The G+C content of the genomic DNA was 67.1 mol%.
32375935	8	36	theme	polar	775:779	arg1	lipids					781:786	The polar lipids	771:786	The polar lipids	771:786	The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid.
32375935	13	37	theme	type	1365:1368	arg1	strains					1370:1376	the type strains	1361:1376	the type strains of Luteipulveratus halotolerans (96.2 %), Branchiibius hedensis (95.4 %), Luteipulveratus mongoliensis (95.4 %) and Branchiibius cervicis (95.3 %)	1361:1523	Highest sequence similarities of the novel isolate were found to the type strains of Luteipulveratus halotolerans (96.2 %), Branchiibius hedensis (95.4 %), Luteipulveratus mongoliensis (95.4 %) and Branchiibius cervicis (95.3 %).
32375935	12	38	theme	distinct	1217:1224	arg1	subcluster					1226:1235	a distinct subcluster	1215:1235	a distinct subcluster	1215:1235	Phylogenetic analyses based on 16S rRNA gene sequences showed that the novel isolate belonged to the family Dermacoccaceae and formed a distinct subcluster at the base of the radiation of the genus Luteipulveratus.
32375935	14	39	theme	family	1622:1627	arg1	Dermacoccaceae					1629:1642	the family Dermacoccaceae	1618:1642	the family Dermacoccaceae	1618:1642	The whole genome-based phylogeny supported the novelty of the isolate at the genus level in the family Dermacoccaceae.
32375935	3	40	theme	short	468:472	arg1	chains					474:479	short chains	468:479	short chains	468:479	The organism was aerobic, and cells were non-spore-forming, non-motile cocci that occurred singly, in pairs, in triplets, in tetrads, in short chains or in irregular clusters.
32375935	15	41	theme	strain	1694:1699	arg1	108676T					1726:1732	=KCTC 39632T=DSM 108676T	1709:1732	=KCTC 39632T=DSM 108676T	1709:1732	On the basis of data from this polyphasic study, strain C5-26T (=KCTC 39632T=DSM 108676T) represents a novel species of a new genus in the family Dermacoccaceae, for which the name Leekyejoonella antrihumi gen. nov., sp.
32375935	15	41	theme	strain	1694:1699	arg1	C5-26T					1701:1706	strain C5-26T	1694:1706	strain C5-26T (=KCTC 39632T=DSM 108676T)	1694:1733	On the basis of data from this polyphasic study, strain C5-26T (=KCTC 39632T=DSM 108676T) represents a novel species of a new genus in the family Dermacoccaceae, for which the name Leekyejoonella antrihumi gen. nov., sp.
32375935	0	42	theme	gen.	25:28	arg1	nov.					30:33	Leekyejoonella antrihumi gen. nov.	0:33	Leekyejoonella antrihumi gen. nov.	0:33	Leekyejoonella antrihumi gen. nov., sp.
32375935	11	43	theme	G+C	1035:1037	arg1	%					1078:1078	67.1 mol%	1070:1078	67.1 mol%	1070:1078	The G+C content of the genomic DNA was 67.1 mol%.
32375935	11	43	theme	G+C	1035:1037	arg1	content					1039:1045	The G+C content	1031:1045	The G+C content of the genomic DNA	1031:1064	The G+C content of the genomic DNA was 67.1 mol%.
32375935	13	44	theme	mongoliensis	1468:1479	arg1	strains					1370:1376	the type strains	1361:1376	the type strains of Luteipulveratus halotolerans (96.2 %), Branchiibius hedensis (95.4 %), Luteipulveratus mongoliensis (95.4 %) and Branchiibius cervicis (95.3 %)	1361:1523	Highest sequence similarities of the novel isolate were found to the type strains of Luteipulveratus halotolerans (96.2 %), Branchiibius hedensis (95.4 %), Luteipulveratus mongoliensis (95.4 %) and Branchiibius cervicis (95.3 %).
32375935	1	45	theme	Dermacoccaceae	73:86	arg1	member					52:57	a new member	46:57	a new member of the family Dermacoccaceae isolated from a cave soil	46:112	nov., a new member of the family Dermacoccaceae isolated from a cave soil.
32375935	1	45	theme	Dermacoccaceae	73:86	arg1	nov.					40:43	nov.	40:43	nov.	40:43	nov., a new member of the family Dermacoccaceae isolated from a cave soil.
32375935	2	46	from	cave	225:228	arg1	Republic					239:246	Republic	239:246	Republic	239:246	A novel Gram-stain-positive, actinobacterial strain, designated C5-26T, was isolated from soil from a natural cave in Jeju, Republic of Korea, and its taxonomic position was investigated using a polyphasic approach.
32375935	12	47	theme	gene	1121:1124	arg1	sequences					1126:1134	16S rRNA gene sequences	1112:1134	16S rRNA gene sequences	1112:1134	Phylogenetic analyses based on 16S rRNA gene sequences showed that the novel isolate belonged to the family Dermacoccaceae and formed a distinct subcluster at the base of the radiation of the genus Luteipulveratus.
32375935	9	48	theme	fatty	909:913	arg1	acids					915:919	The major fatty acids	899:919	The major fatty acids	899:919	The major fatty acids were iso-C16 : 0 and iso-C16 : 1 h.
32375935	9	48	theme	fatty	909:913	arg1	iso-C16 					926:933	iso-C16 	926:933	iso-C16 	926:933	The major fatty acids were iso-C16 : 0 and iso-C16 : 1 h.
32375935	15	49	theme	family	1784:1789	arg1	Dermacoccaceae					1791:1804	the family Dermacoccaceae	1780:1804	the family Dermacoccaceae	1780:1804	On the basis of data from this polyphasic study, strain C5-26T (=KCTC 39632T=DSM 108676T) represents a novel species of a new genus in the family Dermacoccaceae, for which the name Leekyejoonella antrihumi gen. nov., sp.
32375935	13	50	theme	isolate	1339:1345	arg1	similarities					1313:1324	Highest sequence similarities	1296:1324	Highest sequence similarities of the novel isolate	1296:1345	Highest sequence similarities of the novel isolate were found to the type strains of Luteipulveratus halotolerans (96.2 %), Branchiibius hedensis (95.4 %), Luteipulveratus mongoliensis (95.4 %) and Branchiibius cervicis (95.3 %).
32375935	10	51	with	Mbp	995:997	arg1	depth					1004:1008	depth	1004:1008	depth of coverage of 161×	1004:1028	The size of the draft genome was 5.32 Mbp with depth of coverage of 161×.
32375935	12	52	theme	novel	1152:1156	arg1	isolate					1158:1164	the novel isolate	1148:1164	the novel isolate	1148:1164	Phylogenetic analyses based on 16S rRNA gene sequences showed that the novel isolate belonged to the family Dermacoccaceae and formed a distinct subcluster at the base of the radiation of the genus Luteipulveratus.
32375935	5	53	theme	l-Ser-d-Asp	609:619	arg1	bridge					634:639	an l-Ser-d-Asp interpeptide bridge	606:639	an l-Ser-d-Asp interpeptide bridge	606:639	The peptidoglycan type was A4α with an l-Ser-d-Asp interpeptide bridge.
32375935	14	54	from	level	1609:1613	arg1	novelty					1573:1579	the novelty	1569:1579	the novelty of the isolate at the genus level in the family Dermacoccaceae	1569:1642	The whole genome-based phylogeny supported the novelty of the isolate at the genus level in the family Dermacoccaceae.
32375935	14	54	from	level	1609:1613	arg1	Dermacoccaceae					1629:1642	the family Dermacoccaceae	1618:1642	the family Dermacoccaceae	1618:1642	The whole genome-based phylogeny supported the novelty of the isolate at the genus level in the family Dermacoccaceae.
32375935	14	55	theme	genus	1603:1607	arg1	level					1609:1613	the genus level	1599:1613	the genus level in the family Dermacoccaceae	1599:1642	The whole genome-based phylogeny supported the novelty of the isolate at the genus level in the family Dermacoccaceae.
32375935	2	56	theme	Gram-stain-positive	123:141	arg1	strain					160:165	A novel Gram-stain-positive, actinobacterial strain	115:165	A novel Gram-stain-positive, actinobacterial strain	115:165	A novel Gram-stain-positive, actinobacterial strain, designated C5-26T, was isolated from soil from a natural cave in Jeju, Republic of Korea, and its taxonomic position was investigated using a polyphasic approach.
32375935	3	57	dep	non-spore-forming	372:388	arg1	non-motile					391:400	non-motile	391:400	non-motile	391:400	The organism was aerobic, and cells were non-spore-forming, non-motile cocci that occurred singly, in pairs, in triplets, in tetrads, in short chains or in irregular clusters.
32375935	13	58	theme	Highest	1296:1302	arg1	similarities					1313:1324	Highest sequence similarities	1296:1324	Highest sequence similarities of the novel isolate	1296:1345	Highest sequence similarities of the novel isolate were found to the type strains of Luteipulveratus halotolerans (96.2 %), Branchiibius hedensis (95.4 %), Luteipulveratus mongoliensis (95.4 %) and Branchiibius cervicis (95.3 %).
32375935	10	59	theme	161×	1025:1028	arg1	coverage					1013:1020	coverage	1013:1020	coverage of 161×	1013:1028	The size of the draft genome was 5.32 Mbp with depth of coverage of 161×.
32375935	5	60	theme	peptidoglycan	574:586	arg1	type					588:591	The peptidoglycan type	570:591	The peptidoglycan type	570:591	The peptidoglycan type was A4α with an l-Ser-d-Asp interpeptide bridge.
32375935	15	61	theme	39632T=DSM	1715:1724	arg1	108676T					1726:1732	=KCTC 39632T=DSM 108676T	1709:1732	=KCTC 39632T=DSM 108676T	1709:1732	On the basis of data from this polyphasic study, strain C5-26T (=KCTC 39632T=DSM 108676T) represents a novel species of a new genus in the family Dermacoccaceae, for which the name Leekyejoonella antrihumi gen. nov., sp.
32375935	15	61	theme	39632T=DSM	1715:1724	arg1	C5-26T					1701:1706	strain C5-26T	1694:1706	strain C5-26T (=KCTC 39632T=DSM 108676T)	1694:1733	On the basis of data from this polyphasic study, strain C5-26T (=KCTC 39632T=DSM 108676T) represents a novel species of a new genus in the family Dermacoccaceae, for which the name Leekyejoonella antrihumi gen. nov., sp.
32375935	13	62	theme	sequence	1304:1311	arg1	similarities					1313:1324	Highest sequence similarities	1296:1324	Highest sequence similarities of the novel isolate	1296:1345	Highest sequence similarities of the novel isolate were found to the type strains of Luteipulveratus halotolerans (96.2 %), Branchiibius hedensis (95.4 %), Luteipulveratus mongoliensis (95.4 %) and Branchiibius cervicis (95.3 %).
32375935	12	63	theme	family	1182:1187	arg1	Dermacoccaceae					1189:1202	the family Dermacoccaceae	1178:1202	the family Dermacoccaceae	1178:1202	Phylogenetic analyses based on 16S rRNA gene sequences showed that the novel isolate belonged to the family Dermacoccaceae and formed a distinct subcluster at the base of the radiation of the genus Luteipulveratus.
32375935	15	64	theme	new	1767:1769	arg1	genus					1771:1775	a new genus	1765:1775	a new genus in the family Dermacoccaceae	1765:1804	On the basis of data from this polyphasic study, strain C5-26T (=KCTC 39632T=DSM 108676T) represents a novel species of a new genus in the family Dermacoccaceae, for which the name Leekyejoonella antrihumi gen. nov., sp.
32375935	6	65	theme	whole-cell	646:655	arg1	sugars					657:662	The whole-cell sugars	642:662	The whole-cell sugars	642:662	The whole-cell sugars comprised glucose, rhamnose, mannose, arabinose, galactose and ribose.
32375935	2	66	theme	taxonomic	266:274	arg1	position					276:283	its taxonomic position	262:283	its taxonomic position	262:283	A novel Gram-stain-positive, actinobacterial strain, designated C5-26T, was isolated from soil from a natural cave in Jeju, Republic of Korea, and its taxonomic position was investigated using a polyphasic approach.
32375935	1	67	theme	family	66:71	arg1	Dermacoccaceae					73:86	the family Dermacoccaceae	62:86	the family Dermacoccaceae isolated from a cave soil	62:112	nov., a new member of the family Dermacoccaceae isolated from a cave soil.
32375935	14	68	theme	isolate	1588:1594	arg1	novelty					1573:1579	the novelty	1569:1579	the novelty of the isolate at the genus level in the family Dermacoccaceae	1569:1642	The whole genome-based phylogeny supported the novelty of the isolate at the genus level in the family Dermacoccaceae.
32375935	15	69	theme	gen.	1851:1854	arg1	nov.					1856:1859	the name Leekyejoonella antrihumi gen. nov.	1817:1859	the name Leekyejoonella antrihumi gen. nov.	1817:1859	On the basis of data from this polyphasic study, strain C5-26T (=KCTC 39632T=DSM 108676T) represents a novel species of a new genus in the family Dermacoccaceae, for which the name Leekyejoonella antrihumi gen. nov., sp.
32375935	15	70	from	study	1687:1691	arg1	data					1661:1664	data	1661:1664	data from this polyphasic study	1661:1691	On the basis of data from this polyphasic study, strain C5-26T (=KCTC 39632T=DSM 108676T) represents a novel species of a new genus in the family Dermacoccaceae, for which the name Leekyejoonella antrihumi gen. nov., sp.
32375935	2	71	theme	natural	217:223	arg1	cave					225:228	a natural cave	215:228	a natural cave in Jeju, Republic of Korea	215:255	A novel Gram-stain-positive, actinobacterial strain, designated C5-26T, was isolated from soil from a natural cave in Jeju, Republic of Korea, and its taxonomic position was investigated using a polyphasic approach.
32375935	1	72	theme	cave	104:107	arg1	soil					109:112	a cave soil	102:112	a cave soil	102:112	nov., a new member of the family Dermacoccaceae isolated from a cave soil.
32375935	12	73	theme	rRNA	1116:1119	arg1	sequences					1126:1134	16S rRNA gene sequences	1112:1134	16S rRNA gene sequences	1112:1134	Phylogenetic analyses based on 16S rRNA gene sequences showed that the novel isolate belonged to the family Dermacoccaceae and formed a distinct subcluster at the base of the radiation of the genus Luteipulveratus.
32375935	15	74	theme	antrihumi	1841:1849	arg1	nov.					1856:1859	the name Leekyejoonella antrihumi gen. nov.	1817:1859	the name Leekyejoonella antrihumi gen. nov.	1817:1859	On the basis of data from this polyphasic study, strain C5-26T (=KCTC 39632T=DSM 108676T) represents a novel species of a new genus in the family Dermacoccaceae, for which the name Leekyejoonella antrihumi gen. nov., sp.
32375935	15	75	dep	sp	1862:1863	arg1	nov.					1856:1859	the name Leekyejoonella antrihumi gen. nov.	1817:1859	the name Leekyejoonella antrihumi gen. nov.	1817:1859	On the basis of data from this polyphasic study, strain C5-26T (=KCTC 39632T=DSM 108676T) represents a novel species of a new genus in the family Dermacoccaceae, for which the name Leekyejoonella antrihumi gen. nov., sp.
32375935	2	76	theme	polyphasic	310:319	arg1	approach					321:328	a polyphasic approach	308:328	a polyphasic approach	308:328	A novel Gram-stain-positive, actinobacterial strain, designated C5-26T, was isolated from soil from a natural cave in Jeju, Republic of Korea, and its taxonomic position was investigated using a polyphasic approach.
32375935	15	77	from	Dermacoccaceae	1791:1804	arg1	species					1754:1760	a novel species	1746:1760	a novel species	1746:1760	On the basis of data from this polyphasic study, strain C5-26T (=KCTC 39632T=DSM 108676T) represents a novel species of a new genus in the family Dermacoccaceae, for which the name Leekyejoonella antrihumi gen. nov., sp.
32375935	15	78	theme	polyphasic	1676:1685	arg1	study					1687:1691	this polyphasic study	1671:1691	this polyphasic study	1671:1691	On the basis of data from this polyphasic study, strain C5-26T (=KCTC 39632T=DSM 108676T) represents a novel species of a new genus in the family Dermacoccaceae, for which the name Leekyejoonella antrihumi gen. nov., sp.
32375935	10	79	theme	draft	973:977	arg1	genome					979:984	the draft genome	969:984	the draft genome	969:984	The size of the draft genome was 5.32 Mbp with depth of coverage of 161×.
32375935	9	80	theme	major	903:907	arg1	acids					915:919	The major fatty acids	899:919	The major fatty acids	899:919	The major fatty acids were iso-C16 : 0 and iso-C16 : 1 h.
32375935	9	80	theme	major	903:907	arg1	iso-C16 					926:933	iso-C16 	926:933	iso-C16 	926:933	The major fatty acids were iso-C16 : 0 and iso-C16 : 1 h.
32375935	8	81	theme	unidentified	872:883	arg1	phospholipid					885:896	an unidentified phospholipid	869:896	an unidentified phospholipid	869:896	The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid.
32375935	15	82	theme	=KCTC	1709:1713	arg1	108676T					1726:1732	=KCTC 39632T=DSM 108676T	1709:1732	=KCTC 39632T=DSM 108676T	1709:1732	On the basis of data from this polyphasic study, strain C5-26T (=KCTC 39632T=DSM 108676T) represents a novel species of a new genus in the family Dermacoccaceae, for which the name Leekyejoonella antrihumi gen. nov., sp.
32375935	15	82	theme	=KCTC	1709:1713	arg1	C5-26T					1701:1706	strain C5-26T	1694:1706	strain C5-26T (=KCTC 39632T=DSM 108676T)	1694:1733	On the basis of data from this polyphasic study, strain C5-26T (=KCTC 39632T=DSM 108676T) represents a novel species of a new genus in the family Dermacoccaceae, for which the name Leekyejoonella antrihumi gen. nov., sp.
32375935	15	83	theme	genus	1771:1775	arg1	species					1754:1760	a novel species	1746:1760	a novel species	1746:1760	On the basis of data from this polyphasic study, strain C5-26T (=KCTC 39632T=DSM 108676T) represents a novel species of a new genus in the family Dermacoccaceae, for which the name Leekyejoonella antrihumi gen. nov., sp.
32375935	4	84	theme	cells	523:527	arg1	Colonies					507:514	Colonies	507:514	Colonies of the cells	507:527	Colonies of the cells were circular, convex, entire and white.
32223833	3	0	theme	16S	265:267	arg1	rRNA					269:272	16S rRNA	265:272	16S rRNA gene sequence analysis	265:295	16S rRNA gene sequence analysis revealed that the new isolate was most closely related to Micromonospora craniellae LHW 63014T (99.3 % similarity).
32223833	4	1	theme	strain	508:513	arg1	KJ-029T					515:521	strain KJ-029T	508:521	strain KJ-029T	508:521	Phylogenetic analyses of the genus Micromonospora based on 16S rRNA gene sequences showed that strain KJ-029T was clustered with Micromonospora craniellae LHW 63014T and Micromonospora endophytica 202201T.
32223833	11	2	theme	fatty	1264:1268	arg1	acid					1270:1273	The predominant cellular fatty acid	1239:1273	The predominant cellular fatty acid	1239:1273	The predominant cellular fatty acid was iso-C16 : 0.
32223833	11	2	theme	fatty	1264:1268	arg1	 0					1288:1289	 0	1288:1289	 0	1288:1289	The predominant cellular fatty acid was iso-C16 : 0.
32223833	15	3	theme	=NBRC	1553:1557	arg1	9233T					1572:1576	=NBRC 113519T=TBRC 9233T	1553:1576	=NBRC 113519T=TBRC 9233T	1553:1576	The type strain is KJ-029T (=NBRC 113519T=TBRC 9233T).
32223833	15	3	theme	=NBRC	1553:1557	arg1	KJ-029T					1544:1550	KJ-029T	1544:1550	KJ-029T (=NBRC 113519T=TBRC 9233T)	1544:1577	The type strain is KJ-029T (=NBRC 113519T=TBRC 9233T).
32223833	3	4	theme	rRNA	269:272	arg1	analysis					288:295	16S rRNA gene sequence analysis	265:295	16S rRNA gene sequence analysis	265:295	16S rRNA gene sequence analysis revealed that the new isolate was most closely related to Micromonospora craniellae LHW 63014T (99.3 % similarity).
32223833	15	5	theme	type	1529:1532	arg1	strain					1534:1539	The type strain	1525:1539	The type strain	1525:1539	The type strain is KJ-029T (=NBRC 113519T=TBRC 9233T).
32223833	15	5	theme	type	1529:1532	arg1	KJ-029T					1544:1550	KJ-029T	1544:1550	KJ-029T (=NBRC 113519T=TBRC 9233T)	1544:1577	The type strain is KJ-029T (=NBRC 113519T=TBRC 9233T).
32223833	7	6	theme	acyl	950:953	arg1	glycolyl					985:992	glycolyl	985:992	glycolyl	985:992	The acyl type of the peptidoglycan was glycolyl and mycolic acids were absent.
32223833	7	6	theme	acyl	950:953	arg1	type					955:958	The acyl type	946:958	The acyl type of the peptidoglycan	946:979	The acyl type of the peptidoglycan was glycolyl and mycolic acids were absent.
32223833	6	7	contain	contained	810:818	arg1	strain					803:808	The novel strain	793:808	The novel strain	793:808	The novel strain contained meso-diaminopimelic acid and 3-OH-diaminopimelic acid, d-glutamic acid, glycine and d-alanine in the cell-wall peptidoglycan.
32223833	6	7	contain	contained	810:818	arg2	acid					869:872	3-OH-diaminopimelic acid	849:872	3-OH-diaminopimelic acid	849:872	The novel strain contained meso-diaminopimelic acid and 3-OH-diaminopimelic acid, d-glutamic acid, glycine and d-alanine in the cell-wall peptidoglycan.
32223833	6	7	contain	contained	810:818	arg2	acid					840:843	meso-diaminopimelic acid	820:843	meso-diaminopimelic acid	820:843	The novel strain contained meso-diaminopimelic acid and 3-OH-diaminopimelic acid, d-glutamic acid, glycine and d-alanine in the cell-wall peptidoglycan.
32223833	10	8	theme	major	1178:1182	arg1	Phosphatidylethanolamine					1133:1156	Phosphatidylethanolamine	1133:1156	Phosphatidylethanolamine	1133:1156	Phosphatidylethanolamine was detected as the major phospholipid and corresponded to phospholipid type II.
32223833	10	8	theme	major	1178:1182	arg1	phospholipid					1184:1195	the major phospholipid	1174:1195	the major phospholipid	1174:1195	Phosphatidylethanolamine was detected as the major phospholipid and corresponded to phospholipid type II.
32223833	4	9	theme	Micromonospora	448:461	arg1	analyses					426:433	Phylogenetic analyses	413:433	Phylogenetic analyses of the genus Micromonospora based on 16S rRNA gene sequences	413:494	Phylogenetic analyses of the genus Micromonospora based on 16S rRNA gene sequences showed that strain KJ-029T was clustered with Micromonospora craniellae LHW 63014T and Micromonospora endophytica 202201T.
32223833	11	10	theme	predominant	1243:1253	arg1	acid					1270:1273	The predominant cellular fatty acid	1239:1273	The predominant cellular fatty acid	1239:1273	The predominant cellular fatty acid was iso-C16 : 0.
32223833	11	10	theme	predominant	1243:1253	arg1	 0					1288:1289	 0	1288:1289	 0	1288:1289	The predominant cellular fatty acid was iso-C16 : 0.
32223833	12	11	theme	genomic	1319:1325	arg1	DNA					1327:1329	the genomic DNA	1315:1329	the genomic DNA	1315:1329	The DNA G+C content of the genomic DNA was 71.5 mol%.
32223833	12	12	theme	71.5 mol	1335:1342	arg1	%					1343:1343	71.5 mol%	1335:1343	71.5 mol%	1335:1343	The DNA G+C content of the genomic DNA was 71.5 mol%.
32223833	12	12	theme	71.5 mol	1335:1342	arg1	content					1304:1310	The DNA G+C content	1292:1310	The DNA G+C content of the genomic DNA	1292:1329	The DNA G+C content of the genomic DNA was 71.5 mol%.
32223833	6	13	from	acid	869:872	arg1	peptidoglycan					931:943	the cell-wall peptidoglycan	917:943	the cell-wall peptidoglycan	917:943	The novel strain contained meso-diaminopimelic acid and 3-OH-diaminopimelic acid, d-glutamic acid, glycine and d-alanine in the cell-wall peptidoglycan.
32223833	4	14	theme	Phylogenetic	413:424	arg1	analyses					426:433	Phylogenetic analyses	413:433	Phylogenetic analyses of the genus Micromonospora based on 16S rRNA gene sequences	413:494	Phylogenetic analyses of the genus Micromonospora based on 16S rRNA gene sequences showed that strain KJ-029T was clustered with Micromonospora craniellae LHW 63014T and Micromonospora endophytica 202201T.
32223833	5	15	theme	digital	628:634	arg1	hybridization					644:656	digital DNA-DNA hybridization	628:656	digital DNA-DNA hybridization analyses	628:665	However, digital DNA-DNA hybridization analyses presented low levels of relatedness in the range of 24.8-32.9 % between strain KJ-029T and the above closely related strains.
32223833	15	16	theme	113519T=TBRC	1559:1570	arg1	9233T					1572:1576	=NBRC 113519T=TBRC 9233T	1553:1576	=NBRC 113519T=TBRC 9233T	1553:1576	The type strain is KJ-029T (=NBRC 113519T=TBRC 9233T).
32223833	15	16	theme	113519T=TBRC	1559:1570	arg1	KJ-029T					1544:1550	KJ-029T	1544:1550	KJ-029T (=NBRC 113519T=TBRC 9233T)	1544:1577	The type strain is KJ-029T (=NBRC 113519T=TBRC 9233T).
32223833	13	17	theme	genus	1434:1438	arg1	Micromonospora					1440:1453	the genus Micromonospora	1430:1453	the genus Micromonospora	1430:1453	Based on the present polyphasic study, strain KJ-029T represents a novel species of the genus Micromonospora, for which the name Micromonospora pelagivivens sp.
32223833	6	18	from	acid	886:889	arg1	peptidoglycan					931:943	the cell-wall peptidoglycan	917:943	the cell-wall peptidoglycan	917:943	The novel strain contained meso-diaminopimelic acid and 3-OH-diaminopimelic acid, d-glutamic acid, glycine and d-alanine in the cell-wall peptidoglycan.
32223833	2	19	theme	marine	130:135	arg1	actinomycete					137:148	A novel marine actinomycete	122:148	A novel marine actinomycete	122:148	A novel marine actinomycete, designated strain KJ-029T, was isolated from a marine sediment sample (water depth of 226 m) in Kagoshima, Japan.
32223833	2	20	theme	m	241:241	arg1	depth					228:232	water depth	222:232	water depth of 226 m	222:241	A novel marine actinomycete, designated strain KJ-029T, was isolated from a marine sediment sample (water depth of 226 m) in Kagoshima, Japan.
32223833	2	21	attach	isolated	182:189	arg2	actinomycete					137:148	A novel marine actinomycete	122:148	A novel marine actinomycete	122:148	A novel marine actinomycete, designated strain KJ-029T, was isolated from a marine sediment sample (water depth of 226 m) in Kagoshima, Japan.
32223833	2	21	attach	isolated	182:189	arg1	sample					214:219	a marine sediment sample	196:219	a marine sediment sample (water depth of 226 m)	196:242	A novel marine actinomycete, designated strain KJ-029T, was isolated from a marine sediment sample (water depth of 226 m) in Kagoshima, Japan.
32223833	2	22	theme	novel	124:128	arg1	actinomycete					137:148	A novel marine actinomycete	122:148	A novel marine actinomycete	122:148	A novel marine actinomycete, designated strain KJ-029T, was isolated from a marine sediment sample (water depth of 226 m) in Kagoshima, Japan.
32223833	8	23	theme	major	1029:1033	arg1	menaquinone					1035:1045	The major menaquinone	1025:1045	The major menaquinone	1025:1045	The major menaquinone was MK-9(H4).
32223833	8	23	theme	major	1029:1033	arg1	MK-9					1051:1054	MK-9	1051:1054	MK-9(H4)	1051:1058	The major menaquinone was MK-9(H4).
32223833	6	24	theme	meso-diaminopimelic	820:838	arg1	acid					840:843	meso-diaminopimelic acid	820:843	meso-diaminopimelic acid	820:843	The novel strain contained meso-diaminopimelic acid and 3-OH-diaminopimelic acid, d-glutamic acid, glycine and d-alanine in the cell-wall peptidoglycan.
32223833	1	25	theme	genus	59:63	arg1	Micromonospora					65:78	the genus Micromonospora	55:78	the genus Micromonospora isolated from deep-sea sediment in Japan	55:119	nov., a new species of the genus Micromonospora isolated from deep-sea sediment in Japan.
32223833	13	26	theme	Micromonospora	1440:1453	arg1	species					1419:1425	a novel species	1411:1425	a novel species	1411:1425	Based on the present polyphasic study, strain KJ-029T represents a novel species of the genus Micromonospora, for which the name Micromonospora pelagivivens sp.
32223833	6	27	from	acid	840:843	arg1	peptidoglycan					931:943	the cell-wall peptidoglycan	917:943	the cell-wall peptidoglycan	917:943	The novel strain contained meso-diaminopimelic acid and 3-OH-diaminopimelic acid, d-glutamic acid, glycine and d-alanine in the cell-wall peptidoglycan.
32223833	5	28	dep	KJ-029T	746:752	arg1	strains					784:790	closely related strains	768:790	closely related strains	768:790	However, digital DNA-DNA hybridization analyses presented low levels of relatedness in the range of 24.8-32.9 % between strain KJ-029T and the above closely related strains.
32223833	6	29	from	glycine	892:898	arg1	peptidoglycan					931:943	the cell-wall peptidoglycan	917:943	the cell-wall peptidoglycan	917:943	The novel strain contained meso-diaminopimelic acid and 3-OH-diaminopimelic acid, d-glutamic acid, glycine and d-alanine in the cell-wall peptidoglycan.
32223833	1	30	theme	Micromonospora	65:78	arg1	species					44:50	a new species	38:50	a new species of the genus Micromonospora isolated from deep-sea sediment in Japan	38:119	nov., a new species of the genus Micromonospora isolated from deep-sea sediment in Japan.
32223833	1	30	theme	Micromonospora	65:78	arg1	nov.					32:35	nov.	32:35	nov.	32:35	nov., a new species of the genus Micromonospora isolated from deep-sea sediment in Japan.
32223833	6	31	theme	d-glutamic	875:884	arg1	acid					886:889	d-glutamic acid	875:889	d-glutamic acid	875:889	The novel strain contained meso-diaminopimelic acid and 3-OH-diaminopimelic acid, d-glutamic acid, glycine and d-alanine in the cell-wall peptidoglycan.
32223833	3	32	theme	craniellae	370:379	arg1	63014T					385:390	Micromonospora craniellae LHW 63014T	355:390	Micromonospora craniellae LHW 63014T (99.3 % similarity)	355:410	16S rRNA gene sequence analysis revealed that the new isolate was most closely related to Micromonospora craniellae LHW 63014T (99.3 % similarity).
32223833	3	32	theme	craniellae	370:379	arg1	similarity					400:409	99.3 % similarity	393:409	99.3 % similarity	393:409	16S rRNA gene sequence analysis revealed that the new isolate was most closely related to Micromonospora craniellae LHW 63014T (99.3 % similarity).
32223833	5	33	theme	related	776:782	arg1	strains					784:790	closely related strains	768:790	closely related strains	768:790	However, digital DNA-DNA hybridization analyses presented low levels of relatedness in the range of 24.8-32.9 % between strain KJ-029T and the above closely related strains.
32223833	1	34	from	sediment	103:110	arg1	Japan					115:119	Japan	115:119	Japan	115:119	nov., a new species of the genus Micromonospora isolated from deep-sea sediment in Japan.
32223833	11	35	theme	cellular	1255:1262	arg1	acid					1270:1273	The predominant cellular fatty acid	1239:1273	The predominant cellular fatty acid	1239:1273	The predominant cellular fatty acid was iso-C16 : 0.
32223833	11	35	theme	cellular	1255:1262	arg1	 0					1288:1289	 0	1288:1289	 0	1288:1289	The predominant cellular fatty acid was iso-C16 : 0.
32223833	3	36	theme	LHW	381:383	arg1	63014T					385:390	Micromonospora craniellae LHW 63014T	355:390	Micromonospora craniellae LHW 63014T (99.3 % similarity)	355:410	16S rRNA gene sequence analysis revealed that the new isolate was most closely related to Micromonospora craniellae LHW 63014T (99.3 % similarity).
32223833	3	36	theme	LHW	381:383	arg1	similarity					400:409	99.3 % similarity	393:409	99.3 % similarity	393:409	16S rRNA gene sequence analysis revealed that the new isolate was most closely related to Micromonospora craniellae LHW 63014T (99.3 % similarity).
32223833	3	37	theme	new	315:317	arg1	related					344:350	related	344:350	related	344:350	16S rRNA gene sequence analysis revealed that the new isolate was most closely related to Micromonospora craniellae LHW 63014T (99.3 % similarity).
32223833	3	37	theme	new	315:317	arg1	isolate					319:325	the new isolate	311:325	the new isolate	311:325	16S rRNA gene sequence analysis revealed that the new isolate was most closely related to Micromonospora craniellae LHW 63014T (99.3 % similarity).
32223833	12	38	theme	G+C	1300:1302	arg1	%					1343:1343	71.5 mol%	1335:1343	71.5 mol%	1335:1343	The DNA G+C content of the genomic DNA was 71.5 mol%.
32223833	12	38	theme	G+C	1300:1302	arg1	content					1304:1310	The DNA G+C content	1292:1310	The DNA G+C content of the genomic DNA	1292:1329	The DNA G+C content of the genomic DNA was 71.5 mol%.
32223833	5	39	theme	low	677:679	arg1	levels					681:686	low levels	677:686	low levels of relatedness	677:701	However, digital DNA-DNA hybridization analyses presented low levels of relatedness in the range of 24.8-32.9 % between strain KJ-029T and the above closely related strains.
32223833	12	40	theme	DNA	1296:1298	arg1	%					1343:1343	71.5 mol%	1335:1343	71.5 mol%	1335:1343	The DNA G+C content of the genomic DNA was 71.5 mol%.
32223833	12	40	theme	DNA	1296:1298	arg1	content					1304:1310	The DNA G+C content	1292:1310	The DNA G+C content of the genomic DNA	1292:1329	The DNA G+C content of the genomic DNA was 71.5 mol%.
32223833	9	41	theme	whole-cell	1065:1074	arg1	sugars					1076:1081	The whole-cell sugars	1061:1081	The whole-cell sugars	1061:1081	The whole-cell sugars consisted of glucose, mannose, xylose and ribose.
32223833	13	42	theme	novel	1413:1417	arg1	species					1419:1425	a novel species	1411:1425	a novel species	1411:1425	Based on the present polyphasic study, strain KJ-029T represents a novel species of the genus Micromonospora, for which the name Micromonospora pelagivivens sp.
32223833	12	43	theme	DNA	1327:1329	arg1	%					1343:1343	71.5 mol%	1335:1343	71.5 mol%	1335:1343	The DNA G+C content of the genomic DNA was 71.5 mol%.
32223833	12	43	theme	DNA	1327:1329	arg1	content					1304:1310	The DNA G+C content	1292:1310	The DNA G+C content of the genomic DNA	1292:1329	The DNA G+C content of the genomic DNA was 71.5 mol%.
32223833	2	44	dep	sample	214:219	arg1	depth					228:232	water depth	222:232	water depth of 226 m	222:241	A novel marine actinomycete, designated strain KJ-029T, was isolated from a marine sediment sample (water depth of 226 m) in Kagoshima, Japan.
32223833	6	45	theme	3-OH-diaminopimelic	849:867	arg1	acid					869:872	3-OH-diaminopimelic acid	849:872	3-OH-diaminopimelic acid	849:872	The novel strain contained meso-diaminopimelic acid and 3-OH-diaminopimelic acid, d-glutamic acid, glycine and d-alanine in the cell-wall peptidoglycan.
32223833	1	46	theme	deep-sea	94:101	arg1	sediment					103:110	deep-sea sediment	94:110	deep-sea sediment in Japan	94:119	nov., a new species of the genus Micromonospora isolated from deep-sea sediment in Japan.
32223833	5	47	attach	presented	667:675	arg1	range					710:714	the range	706:714	the range of 24.8-32.9 % between strain KJ-029T and the above closely related strains	706:790	However, digital DNA-DNA hybridization analyses presented low levels of relatedness in the range of 24.8-32.9 % between strain KJ-029T and the above closely related strains.
32223833	5	47	attach	presented	667:675	arg2	analyses					658:665	digital DNA-DNA hybridization analyses	628:665	digital DNA-DNA hybridization analyses	628:665	However, digital DNA-DNA hybridization analyses presented low levels of relatedness in the range of 24.8-32.9 % between strain KJ-029T and the above closely related strains.
32223833	2	48	theme	strain	162:167	arg1	KJ-029T					169:175	strain KJ-029T	162:175	strain KJ-029T	162:175	A novel marine actinomycete, designated strain KJ-029T, was isolated from a marine sediment sample (water depth of 226 m) in Kagoshima, Japan.
32223833	3	49	theme	99.3 	393:397	arg1	%					398:398	%	398:398	%	398:398	16S rRNA gene sequence analysis revealed that the new isolate was most closely related to Micromonospora craniellae LHW 63014T (99.3 % similarity).
32223833	3	50	theme	Micromonospora	355:368	arg1	63014T					385:390	Micromonospora craniellae LHW 63014T	355:390	Micromonospora craniellae LHW 63014T (99.3 % similarity)	355:410	16S rRNA gene sequence analysis revealed that the new isolate was most closely related to Micromonospora craniellae LHW 63014T (99.3 % similarity).
32223833	3	50	theme	Micromonospora	355:368	arg1	similarity					400:409	99.3 % similarity	393:409	99.3 % similarity	393:409	16S rRNA gene sequence analysis revealed that the new isolate was most closely related to Micromonospora craniellae LHW 63014T (99.3 % similarity).
32223833	1	51	attach	isolated	80:87	arg1	sediment					103:110	deep-sea sediment	94:110	deep-sea sediment in Japan	94:119	nov., a new species of the genus Micromonospora isolated from deep-sea sediment in Japan.
32223833	1	51	attach	isolated	80:87	arg2	Micromonospora					65:78	the genus Micromonospora	55:78	the genus Micromonospora isolated from deep-sea sediment in Japan	55:119	nov., a new species of the genus Micromonospora isolated from deep-sea sediment in Japan.
32223833	5	52	theme	relatedness	691:701	arg1	levels					681:686	low levels	677:686	low levels of relatedness	677:701	However, digital DNA-DNA hybridization analyses presented low levels of relatedness in the range of 24.8-32.9 % between strain KJ-029T and the above closely related strains.
32223833	2	53	theme	sediment	205:212	arg1	sample					214:219	a marine sediment sample	196:219	a marine sediment sample (water depth of 226 m)	196:242	A novel marine actinomycete, designated strain KJ-029T, was isolated from a marine sediment sample (water depth of 226 m) in Kagoshima, Japan.
32223833	7	54	theme	mycolic	998:1004	arg1	acids					1006:1010	mycolic acids	998:1010	mycolic acids	998:1010	The acyl type of the peptidoglycan was glycolyl and mycolic acids were absent.
32223833	3	55	theme	%	398:398	arg1	63014T					385:390	Micromonospora craniellae LHW 63014T	355:390	Micromonospora craniellae LHW 63014T (99.3 % similarity)	355:410	16S rRNA gene sequence analysis revealed that the new isolate was most closely related to Micromonospora craniellae LHW 63014T (99.3 % similarity).
32223833	3	55	theme	%	398:398	arg1	similarity					400:409	99.3 % similarity	393:409	99.3 % similarity	393:409	16S rRNA gene sequence analysis revealed that the new isolate was most closely related to Micromonospora craniellae LHW 63014T (99.3 % similarity).
32223833	4	56	theme	16S	472:474	arg1	sequences					486:494	16S rRNA gene sequences	472:494	16S rRNA gene sequences	472:494	Phylogenetic analyses of the genus Micromonospora based on 16S rRNA gene sequences showed that strain KJ-029T was clustered with Micromonospora craniellae LHW 63014T and Micromonospora endophytica 202201T.
32223833	5	57	theme	DNA-DNA	636:642	arg1	hybridization					644:656	digital DNA-DNA hybridization	628:656	digital DNA-DNA hybridization analyses	628:665	However, digital DNA-DNA hybridization analyses presented low levels of relatedness in the range of 24.8-32.9 % between strain KJ-029T and the above closely related strains.
32223833	5	58	theme	%	729:729	arg1	range					710:714	the range	706:714	the range of 24.8-32.9 % between strain KJ-029T and the above closely related strains	706:790	However, digital DNA-DNA hybridization analyses presented low levels of relatedness in the range of 24.8-32.9 % between strain KJ-029T and the above closely related strains.
32223833	2	59	theme	marine	198:203	arg1	sample					214:219	a marine sediment sample	196:219	a marine sediment sample (water depth of 226 m)	196:242	A novel marine actinomycete, designated strain KJ-029T, was isolated from a marine sediment sample (water depth of 226 m) in Kagoshima, Japan.
32223833	13	60	theme	strain	1385:1390	arg1	KJ-029T					1392:1398	strain KJ-029T	1385:1398	strain KJ-029T	1385:1398	Based on the present polyphasic study, strain KJ-029T represents a novel species of the genus Micromonospora, for which the name Micromonospora pelagivivens sp.
32223833	4	61	theme	gene	481:484	arg1	sequences					486:494	16S rRNA gene sequences	472:494	16S rRNA gene sequences	472:494	Phylogenetic analyses of the genus Micromonospora based on 16S rRNA gene sequences showed that strain KJ-029T was clustered with Micromonospora craniellae LHW 63014T and Micromonospora endophytica 202201T.
32223833	5	62	theme	hybridization	644:656	arg1	analyses					658:665	digital DNA-DNA hybridization analyses	628:665	digital DNA-DNA hybridization analyses	628:665	However, digital DNA-DNA hybridization analyses presented low levels of relatedness in the range of 24.8-32.9 % between strain KJ-029T and the above closely related strains.
32223833	10	63	theme	phospholipid	1217:1228	arg1	type					1230:1233	phospholipid type II	1217:1236	phospholipid type II	1217:1236	Phosphatidylethanolamine was detected as the major phospholipid and corresponded to phospholipid type II.
32223833	4	64	theme	rRNA	476:479	arg1	sequences					486:494	16S rRNA gene sequences	472:494	16S rRNA gene sequences	472:494	Phylogenetic analyses of the genus Micromonospora based on 16S rRNA gene sequences showed that strain KJ-029T was clustered with Micromonospora craniellae LHW 63014T and Micromonospora endophytica 202201T.
32223833	13	65	theme	name	1470:1473	arg1	Micromonospora					1475:1488	the name Micromonospora	1466:1488	the name Micromonospora	1466:1488	Based on the present polyphasic study, strain KJ-029T represents a novel species of the genus Micromonospora, for which the name Micromonospora pelagivivens sp.
32223833	6	66	theme	novel	797:801	arg1	strain					803:808	The novel strain	793:808	The novel strain	793:808	The novel strain contained meso-diaminopimelic acid and 3-OH-diaminopimelic acid, d-glutamic acid, glycine and d-alanine in the cell-wall peptidoglycan.
32223833	7	67	theme	peptidoglycan	967:979	arg1	glycolyl					985:992	glycolyl	985:992	glycolyl	985:992	The acyl type of the peptidoglycan was glycolyl and mycolic acids were absent.
32223833	7	67	theme	peptidoglycan	967:979	arg1	type					955:958	The acyl type	946:958	The acyl type of the peptidoglycan	946:979	The acyl type of the peptidoglycan was glycolyl and mycolic acids were absent.
32223833	3	68	theme	gene	274:277	arg1	analysis					288:295	16S rRNA gene sequence analysis	265:295	16S rRNA gene sequence analysis	265:295	16S rRNA gene sequence analysis revealed that the new isolate was most closely related to Micromonospora craniellae LHW 63014T (99.3 % similarity).
32223833	2	69	theme	water	222:226	arg1	depth					228:232	water depth	222:232	water depth of 226 m	222:241	A novel marine actinomycete, designated strain KJ-029T, was isolated from a marine sediment sample (water depth of 226 m) in Kagoshima, Japan.
32223833	13	70	theme	present	1359:1365	arg1	study					1378:1382	the present polyphasic study	1355:1382	the present polyphasic study	1355:1382	Based on the present polyphasic study, strain KJ-029T represents a novel species of the genus Micromonospora, for which the name Micromonospora pelagivivens sp.
32223833	3	71	theme	sequence	279:286	arg1	analysis					288:295	16S rRNA gene sequence analysis	265:295	16S rRNA gene sequence analysis	265:295	16S rRNA gene sequence analysis revealed that the new isolate was most closely related to Micromonospora craniellae LHW 63014T (99.3 % similarity).
32223833	4	72	theme	genus	442:446	arg1	Micromonospora					448:461	the genus Micromonospora	438:461	the genus Micromonospora based on 16S rRNA gene sequences	438:494	Phylogenetic analyses of the genus Micromonospora based on 16S rRNA gene sequences showed that strain KJ-029T was clustered with Micromonospora craniellae LHW 63014T and Micromonospora endophytica 202201T.
32223833	1	73	theme	new	40:42	arg1	species					44:50	a new species	38:50	a new species of the genus Micromonospora isolated from deep-sea sediment in Japan	38:119	nov., a new species of the genus Micromonospora isolated from deep-sea sediment in Japan.
32223833	1	73	theme	new	40:42	arg1	nov.					32:35	nov.	32:35	nov.	32:35	nov., a new species of the genus Micromonospora isolated from deep-sea sediment in Japan.
32223833	13	74	theme	polyphasic	1367:1376	arg1	study					1378:1382	the present polyphasic study	1355:1382	the present polyphasic study	1355:1382	Based on the present polyphasic study, strain KJ-029T represents a novel species of the genus Micromonospora, for which the name Micromonospora pelagivivens sp.
32223833	6	75	from	d-alanine	904:912	arg1	peptidoglycan					931:943	the cell-wall peptidoglycan	917:943	the cell-wall peptidoglycan	917:943	The novel strain contained meso-diaminopimelic acid and 3-OH-diaminopimelic acid, d-glutamic acid, glycine and d-alanine in the cell-wall peptidoglycan.
32223833	6	76	theme	cell-wall	921:929	arg1	peptidoglycan					931:943	the cell-wall peptidoglycan	917:943	the cell-wall peptidoglycan	917:943	The novel strain contained meso-diaminopimelic acid and 3-OH-diaminopimelic acid, d-glutamic acid, glycine and d-alanine in the cell-wall peptidoglycan.
32193578	12	0	theme	= KCTC	1297:1302	arg1	L9T					1292:1294	L9T	1292:1294	L9T (= KCTC 43176T = CGMCC 1.17659T)	1292:1327	The type strain is L9T (= KCTC 43176T = CGMCC 1.17659T).
32193578	12	0	theme	= KCTC	1297:1302	arg1	1.17659T					1319:1326	= KCTC 43176T = CGMCC 1.17659T	1297:1326	= KCTC 43176T = CGMCC 1.17659T	1297:1326	The type strain is L9T (= KCTC 43176T = CGMCC 1.17659T).
32193578	6	1	theme	unidentified	657:668	arg1	phospholipids					670:682	two unidentified phospholipids	653:682	two unidentified phospholipids	653:682	The phospholipids were dominated by diphosphatidylglycerol, phosphatidylglycerol, one unidentified aminophospholipid and two unidentified phospholipids.
32193578	9	2	theme	strain	1012:1017	arg1	L9T					1019:1021	strain L9T	1012:1021	strain L9T	1012:1021	The ANI and dDDH values between strain L9T and the closely related species were 69.8-76.1% and 13.0-20.5%, respectively.
32193578	10	3	theme	genus	1182:1186	arg1	Ornithinibacillus					1188:1204	the genus Ornithinibacillus	1178:1204	the genus Ornithinibacillus	1178:1204	On the basis of the data presented, strain L9T represents a novel species of the genus Ornithinibacillus, for which the name Ornithinibacillus caprae sp.
32193578	8	4	theme	Ornithinibacillus	926:942	arg1	G8BT					955:958	Ornithinibacillus halophilus G8BT	926:958	Ornithinibacillus halophilus G8BT (97.5% similarity)	926:977	Phylogenetic trees based on the 16S rRNA gene sequences showed the isolate to be closely related to Oceanobacillus limi H9BT (98.2% similarity) and Ornithinibacillus halophilus G8BT (97.5% similarity).
32193578	8	4	theme	Ornithinibacillus	926:942	arg1	similarity					967:976	97.5% similarity	961:976	97.5% similarity	961:976	Phylogenetic trees based on the 16S rRNA gene sequences showed the isolate to be closely related to Oceanobacillus limi H9BT (98.2% similarity) and Ornithinibacillus halophilus G8BT (97.5% similarity).
32193578	4	5	theme	fatty	347:351	arg1	iso-C15:0					378:386	iso-C15:0	378:386	iso-C15:0	378:386	The predominant menaquinone was MK-7, and the major cellular fatty acids were identified as iso-C15:0 and anteiso-C15:0.
32193578	4	5	theme	fatty	347:351	arg1	acids					353:357	the major cellular fatty acids	328:357	the major cellular fatty acids	328:357	The predominant menaquinone was MK-7, and the major cellular fatty acids were identified as iso-C15:0 and anteiso-C15:0.
32193578	4	5	theme	fatty	347:351	arg1	anteiso-C15:0					392:404	anteiso-C15:0	392:404	anteiso-C15:0	392:404	The predominant menaquinone was MK-7, and the major cellular fatty acids were identified as iso-C15:0 and anteiso-C15:0.
32193578	4	6	theme	predominant	290:300	arg1	menaquinone					302:312	The predominant menaquinone	286:312	The predominant menaquinone	286:312	The predominant menaquinone was MK-7, and the major cellular fatty acids were identified as iso-C15:0 and anteiso-C15:0.
32193578	4	6	theme	predominant	290:300	arg1	MK-7					318:321	MK-7	318:321	MK-7	318:321	The predominant menaquinone was MK-7, and the major cellular fatty acids were identified as iso-C15:0 and anteiso-C15:0.
32193578	8	7	theme	Phylogenetic	778:789	arg1	trees					791:795	Phylogenetic trees	778:795	Phylogenetic trees based on the 16S rRNA gene sequences	778:832	Phylogenetic trees based on the 16S rRNA gene sequences showed the isolate to be closely related to Oceanobacillus limi H9BT (98.2% similarity) and Ornithinibacillus halophilus G8BT (97.5% similarity).
32193578	9	8	theme	related	1039:1045	arg1	species					1047:1053	the closely related species	1027:1053	the closely related species	1027:1053	The ANI and dDDH values between strain L9T and the closely related species were 69.8-76.1% and 13.0-20.5%, respectively.
32193578	5	9	theme	diagnostic	508:517	arg1	L-ornithine					478:488	L-ornithine	478:488	L-ornithine	478:488	The peptidoglycan amino acid type was determined to be A4β, containing L-ornithine and D-aspartic as diagnostic amino acids.
32193578	5	9	theme	diagnostic	508:517	arg1	acids					525:529	diagnostic amino acids	508:529	diagnostic amino acids	508:529	The peptidoglycan amino acid type was determined to be A4β, containing L-ornithine and D-aspartic as diagnostic amino acids.
32193578	5	9	theme	diagnostic	508:517	arg1	D-aspartic					494:503	D-aspartic	494:503	D-aspartic	494:503	The peptidoglycan amino acid type was determined to be A4β, containing L-ornithine and D-aspartic as diagnostic amino acids.
32193578	12	10	theme	43176T = CGMCC	1304:1317	arg1	L9T					1292:1294	L9T	1292:1294	L9T (= KCTC 43176T = CGMCC 1.17659T)	1292:1327	The type strain is L9T (= KCTC 43176T = CGMCC 1.17659T).
32193578	12	10	theme	43176T = CGMCC	1304:1317	arg1	1.17659T					1319:1326	= KCTC 43176T = CGMCC 1.17659T	1297:1326	= KCTC 43176T = CGMCC 1.17659T	1297:1326	The type strain is L9T (= KCTC 43176T = CGMCC 1.17659T).
32193578	2	11	from	hides	184:188	arg1	China					207:211	China	207:211	China	207:211	A novel Gram-stain-positive, motile, moderate halophile, strain L9T, was isolated from hides of white goat in China.
32193578	10	12	dep	Ornithinibacillus	1226:1242	arg1	caprae					1244:1249	caprae	1244:1249	caprae	1244:1249	On the basis of the data presented, strain L9T represents a novel species of the genus Ornithinibacillus, for which the name Ornithinibacillus caprae sp.
32193578	3	13	theme	5-10	268:271	arg1	%					272:272	%	272:272	%	272:272	The isolate grew optimally at 30 °C, at pH 7 and with 5-10% (w/v) NaCl.
32193578	10	14	theme	novel	1161:1165	arg1	species					1167:1173	a novel species	1159:1173	a novel species	1159:1173	On the basis of the data presented, strain L9T represents a novel species of the genus Ornithinibacillus, for which the name Ornithinibacillus caprae sp.
32193578	8	15	theme	%	965:965	arg1	G8BT					955:958	Ornithinibacillus halophilus G8BT	926:958	Ornithinibacillus halophilus G8BT (97.5% similarity)	926:977	Phylogenetic trees based on the 16S rRNA gene sequences showed the isolate to be closely related to Oceanobacillus limi H9BT (98.2% similarity) and Ornithinibacillus halophilus G8BT (97.5% similarity).
32193578	8	15	theme	%	965:965	arg1	similarity					967:976	97.5% similarity	961:976	97.5% similarity	961:976	Phylogenetic trees based on the 16S rRNA gene sequences showed the isolate to be closely related to Oceanobacillus limi H9BT (98.2% similarity) and Ornithinibacillus halophilus G8BT (97.5% similarity).
32193578	5	16	theme	amino	519:523	arg1	L-ornithine					478:488	L-ornithine	478:488	L-ornithine	478:488	The peptidoglycan amino acid type was determined to be A4β, containing L-ornithine and D-aspartic as diagnostic amino acids.
32193578	5	16	theme	amino	519:523	arg1	acids					525:529	diagnostic amino acids	508:529	diagnostic amino acids	508:529	The peptidoglycan amino acid type was determined to be A4β, containing L-ornithine and D-aspartic as diagnostic amino acids.
32193578	5	16	theme	amino	519:523	arg1	D-aspartic					494:503	D-aspartic	494:503	D-aspartic	494:503	The peptidoglycan amino acid type was determined to be A4β, containing L-ornithine and D-aspartic as diagnostic amino acids.
32193578	12	17	theme	type	1277:1280	arg1	L9T					1292:1294	L9T	1292:1294	L9T (= KCTC 43176T = CGMCC 1.17659T)	1292:1327	The type strain is L9T (= KCTC 43176T = CGMCC 1.17659T).
32193578	12	17	theme	type	1277:1280	arg1	strain					1282:1287	The type strain	1273:1287	The type strain	1273:1287	The type strain is L9T (= KCTC 43176T = CGMCC 1.17659T).
32193578	3	18	theme	%	272:272	arg1	NaCl					280:283	5-10% (w/v) NaCl	268:283	5-10% (w/v) NaCl	268:283	The isolate grew optimally at 30 °C, at pH 7 and with 5-10% (w/v) NaCl.
32193578	8	19	theme	97.5	961:964	arg1	%					965:965	%	965:965	%	965:965	Phylogenetic trees based on the 16S rRNA gene sequences showed the isolate to be closely related to Oceanobacillus limi H9BT (98.2% similarity) and Ornithinibacillus halophilus G8BT (97.5% similarity).
32193578	7	20	theme	%	775:775	arg1	size					724:727	a genome size	715:727	a genome size of 4.0 Mbp	715:738	Genome sequencing resulted in a genome size of 4.0 Mbp and a DNA G + C content of 35.9 mol%.
32193578	7	20	theme	%	775:775	arg1	content					756:762	a DNA G + C content	744:762	a DNA G + C content of 35.9 mol%	744:775	Genome sequencing resulted in a genome size of 4.0 Mbp and a DNA G + C content of 35.9 mol%.
32193578	2	21	attach	isolated	170:177	arg2	L9T					161:163	strain L9T	154:163	strain L9T	154:163	A novel Gram-stain-positive, motile, moderate halophile, strain L9T, was isolated from hides of white goat in China.
32193578	2	21	attach	isolated	170:177	arg2	motile					126:131	motile	126:131	motile	126:131	A novel Gram-stain-positive, motile, moderate halophile, strain L9T, was isolated from hides of white goat in China.
32193578	2	21	attach	isolated	170:177	arg2	Gram-stain-positive					105:123	A novel Gram-stain-positive	97:123	A novel Gram-stain-positive	97:123	A novel Gram-stain-positive, motile, moderate halophile, strain L9T, was isolated from hides of white goat in China.
32193578	2	21	attach	isolated	170:177	arg2	halophile					143:151	moderate halophile	134:151	moderate halophile	134:151	A novel Gram-stain-positive, motile, moderate halophile, strain L9T, was isolated from hides of white goat in China.
32193578	2	21	attach	isolated	170:177	arg1	hides					184:188	hides	184:188	hides of white goat in China	184:211	A novel Gram-stain-positive, motile, moderate halophile, strain L9T, was isolated from hides of white goat in China.
32193578	0	22	theme	caprae	18:23	arg1	sp					25:26	Ornithinibacillus caprae sp	0:26	Ornithinibacillus caprae sp.	0:27	Ornithinibacillus caprae sp.
32193578	8	23	theme	%	908:908	arg1	similarity					910:919	98.2% similarity	904:919	98.2% similarity	904:919	Phylogenetic trees based on the 16S rRNA gene sequences showed the isolate to be closely related to Oceanobacillus limi H9BT (98.2% similarity) and Ornithinibacillus halophilus G8BT (97.5% similarity).
32193578	8	23	theme	%	908:908	arg1	H9BT					898:901	Oceanobacillus limi H9BT	878:901	Oceanobacillus limi H9BT (98.2% similarity)	878:920	Phylogenetic trees based on the 16S rRNA gene sequences showed the isolate to be closely related to Oceanobacillus limi H9BT (98.2% similarity) and Ornithinibacillus halophilus G8BT (97.5% similarity).
32193578	5	24	theme	peptidoglycan	411:423	arg1	type					436:439	The peptidoglycan amino acid type	407:439	The peptidoglycan amino acid type	407:439	The peptidoglycan amino acid type was determined to be A4β, containing L-ornithine and D-aspartic as diagnostic amino acids.
32193578	5	24	theme	peptidoglycan	411:423	arg1	A4β					462:464	A4β	462:464	A4β	462:464	The peptidoglycan amino acid type was determined to be A4β, containing L-ornithine and D-aspartic as diagnostic amino acids.
32193578	0	25	theme	Ornithinibacillus	0:16	arg1	sp					25:26	Ornithinibacillus caprae sp	0:26	Ornithinibacillus caprae sp.	0:27	Ornithinibacillus caprae sp.
32193578	1	26	attach	isolated	56:63	arg2	halophile					46:54	a moderate halophile	35:54	a moderate halophile isolated from the hides of a white goat	35:94	nov., a moderate halophile isolated from the hides of a white goat.
32193578	1	26	attach	isolated	56:63	arg1	hides					74:78	the hides	70:78	the hides of a white goat	70:94	nov., a moderate halophile isolated from the hides of a white goat.
32193578	9	27	theme	ANI	984:986	arg1	values					997:1002	The ANI and dDDH values	980:1002	The ANI and dDDH values between strain L9T and the closely related species	980:1053	The ANI and dDDH values between strain L9T and the closely related species were 69.8-76.1% and 13.0-20.5%, respectively.
32193578	9	27	theme	ANI	984:986	arg1	%					1069:1069	69.8-76.1%	1060:1069	69.8-76.1%	1060:1069	The ANI and dDDH values between strain L9T and the closely related species were 69.8-76.1% and 13.0-20.5%, respectively.
32193578	8	28	theme	gene	819:822	arg1	sequences					824:832	the 16S rRNA gene sequences	806:832	the 16S rRNA gene sequences	806:832	Phylogenetic trees based on the 16S rRNA gene sequences showed the isolate to be closely related to Oceanobacillus limi H9BT (98.2% similarity) and Ornithinibacillus halophilus G8BT (97.5% similarity).
32193578	7	29	theme	DNA	746:748	arg1	content					756:762	a DNA G + C content	744:762	a DNA G + C content of 35.9 mol%	744:775	Genome sequencing resulted in a genome size of 4.0 Mbp and a DNA G + C content of 35.9 mol%.
32193578	2	30	from	goat	199:202	arg1	China					207:211	China	207:211	China	207:211	A novel Gram-stain-positive, motile, moderate halophile, strain L9T, was isolated from hides of white goat in China.
32193578	10	31	theme	Ornithinibacillus	1226:1242	arg1	sp					1251:1252	the name Ornithinibacillus caprae sp	1217:1252	the name Ornithinibacillus caprae sp	1217:1252	On the basis of the data presented, strain L9T represents a novel species of the genus Ornithinibacillus, for which the name Ornithinibacillus caprae sp.
32193578	7	32	theme	Mbp	736:738	arg1	size					724:727	a genome size	715:727	a genome size of 4.0 Mbp	715:738	Genome sequencing resulted in a genome size of 4.0 Mbp and a DNA G + C content of 35.9 mol%.
32193578	7	32	theme	Mbp	736:738	arg1	content					756:762	a DNA G + C content	744:762	a DNA G + C content of 35.9 mol%	744:775	Genome sequencing resulted in a genome size of 4.0 Mbp and a DNA G + C content of 35.9 mol%.
32193578	2	33	theme	strain	154:159	arg1	Gram-stain-positive					105:123	A novel Gram-stain-positive	97:123	A novel Gram-stain-positive	97:123	A novel Gram-stain-positive, motile, moderate halophile, strain L9T, was isolated from hides of white goat in China.
32193578	2	33	theme	strain	154:159	arg1	L9T					161:163	strain L9T	154:163	strain L9T	154:163	A novel Gram-stain-positive, motile, moderate halophile, strain L9T, was isolated from hides of white goat in China.
32193578	1	34	theme	white	85:89	arg1	goat					91:94	a white goat	83:94	a white goat	83:94	nov., a moderate halophile isolated from the hides of a white goat.
32193578	7	35	theme	G + C	750:754	arg1	content					756:762	a DNA G + C content	744:762	a DNA G + C content of 35.9 mol%	744:775	Genome sequencing resulted in a genome size of 4.0 Mbp and a DNA G + C content of 35.9 mol%.
32193578	9	36	theme	dDDH	992:995	arg1	values					997:1002	The ANI and dDDH values	980:1002	The ANI and dDDH values between strain L9T and the closely related species	980:1053	The ANI and dDDH values between strain L9T and the closely related species were 69.8-76.1% and 13.0-20.5%, respectively.
32193578	9	36	theme	dDDH	992:995	arg1	%					1069:1069	69.8-76.1%	1060:1069	69.8-76.1%	1060:1069	The ANI and dDDH values between strain L9T and the closely related species were 69.8-76.1% and 13.0-20.5%, respectively.
32193578	4	37	theme	cellular	338:345	arg1	iso-C15:0					378:386	iso-C15:0	378:386	iso-C15:0	378:386	The predominant menaquinone was MK-7, and the major cellular fatty acids were identified as iso-C15:0 and anteiso-C15:0.
32193578	4	37	theme	cellular	338:345	arg1	acids					353:357	the major cellular fatty acids	328:357	the major cellular fatty acids	328:357	The predominant menaquinone was MK-7, and the major cellular fatty acids were identified as iso-C15:0 and anteiso-C15:0.
32193578	4	37	theme	cellular	338:345	arg1	anteiso-C15:0					392:404	anteiso-C15:0	392:404	anteiso-C15:0	392:404	The predominant menaquinone was MK-7, and the major cellular fatty acids were identified as iso-C15:0 and anteiso-C15:0.
32193578	2	38	theme	white	193:197	arg1	goat					199:202	white goat	193:202	white goat in China	193:211	A novel Gram-stain-positive, motile, moderate halophile, strain L9T, was isolated from hides of white goat in China.
32193578	2	39	from	China	207:211	arg1	hides					184:188	hides	184:188	hides of white goat in China	184:211	A novel Gram-stain-positive, motile, moderate halophile, strain L9T, was isolated from hides of white goat in China.
32193578	5	40	contain	containing	467:476	arg1	type					436:439	The peptidoglycan amino acid type	407:439	The peptidoglycan amino acid type	407:439	The peptidoglycan amino acid type was determined to be A4β, containing L-ornithine and D-aspartic as diagnostic amino acids.
32193578	5	40	contain	containing	467:476	arg2	acids					525:529	diagnostic amino acids	508:529	diagnostic amino acids	508:529	The peptidoglycan amino acid type was determined to be A4β, containing L-ornithine and D-aspartic as diagnostic amino acids.
32193578	5	40	contain	containing	467:476	arg2	L-ornithine					478:488	L-ornithine	478:488	L-ornithine	478:488	The peptidoglycan amino acid type was determined to be A4β, containing L-ornithine and D-aspartic as diagnostic amino acids.
32193578	5	40	contain	containing	467:476	arg2	D-aspartic					494:503	D-aspartic	494:503	D-aspartic	494:503	The peptidoglycan amino acid type was determined to be A4β, containing L-ornithine and D-aspartic as diagnostic amino acids.
32193578	5	40	contain	containing	467:476	arg1	A4β					462:464	A4β	462:464	A4β	462:464	The peptidoglycan amino acid type was determined to be A4β, containing L-ornithine and D-aspartic as diagnostic amino acids.
32193578	8	41	theme	98.2	904:907	arg1	%					908:908	%	908:908	%	908:908	Phylogenetic trees based on the 16S rRNA gene sequences showed the isolate to be closely related to Oceanobacillus limi H9BT (98.2% similarity) and Ornithinibacillus halophilus G8BT (97.5% similarity).
32193578	7	42	theme	Genome	685:690	arg1	sequencing					692:701	Genome sequencing	685:701	Genome sequencing	685:701	Genome sequencing resulted in a genome size of 4.0 Mbp and a DNA G + C content of 35.9 mol%.
32193578	8	43	dep	Ornithinibacillus	926:942	arg1	halophilus					944:953	halophilus	944:953	halophilus	944:953	Phylogenetic trees based on the 16S rRNA gene sequences showed the isolate to be closely related to Oceanobacillus limi H9BT (98.2% similarity) and Ornithinibacillus halophilus G8BT (97.5% similarity).
32193578	2	44	theme	moderate	134:141	arg1	halophile					143:151	moderate halophile	134:151	moderate halophile	134:151	A novel Gram-stain-positive, motile, moderate halophile, strain L9T, was isolated from hides of white goat in China.
32193578	2	44	theme	moderate	134:141	arg1	Gram-stain-positive					105:123	A novel Gram-stain-positive	97:123	A novel Gram-stain-positive	97:123	A novel Gram-stain-positive, motile, moderate halophile, strain L9T, was isolated from hides of white goat in China.
32193578	1	45	theme	goat	91:94	arg1	hides					74:78	the hides	70:78	the hides of a white goat	70:94	nov., a moderate halophile isolated from the hides of a white goat.
32193578	7	46	theme	genome	717:722	arg1	size					724:727	a genome size	715:727	a genome size of 4.0 Mbp	715:738	Genome sequencing resulted in a genome size of 4.0 Mbp and a DNA G + C content of 35.9 mol%.
32193578	3	47	dep	%	272:272	arg1	w/v					275:277	w/v	275:277	w/v	275:277	The isolate grew optimally at 30 °C, at pH 7 and with 5-10% (w/v) NaCl.
32193578	1	48	theme	moderate	37:44	arg1	halophile					46:54	a moderate halophile	35:54	a moderate halophile isolated from the hides of a white goat	35:94	nov., a moderate halophile isolated from the hides of a white goat.
32193578	8	49	theme	limi	893:896	arg1	similarity					910:919	98.2% similarity	904:919	98.2% similarity	904:919	Phylogenetic trees based on the 16S rRNA gene sequences showed the isolate to be closely related to Oceanobacillus limi H9BT (98.2% similarity) and Ornithinibacillus halophilus G8BT (97.5% similarity).
32193578	8	49	theme	limi	893:896	arg1	H9BT					898:901	Oceanobacillus limi H9BT	878:901	Oceanobacillus limi H9BT (98.2% similarity)	878:920	Phylogenetic trees based on the 16S rRNA gene sequences showed the isolate to be closely related to Oceanobacillus limi H9BT (98.2% similarity) and Ornithinibacillus halophilus G8BT (97.5% similarity).
32193578	1	50	dep	halophile	46:54	arg1	nov.					29:32	nov.	29:32	nov.	29:32	nov., a moderate halophile isolated from the hides of a white goat.
32193578	10	51	theme	name	1221:1224	arg1	sp					1251:1252	the name Ornithinibacillus caprae sp	1217:1252	the name Ornithinibacillus caprae sp	1217:1252	On the basis of the data presented, strain L9T represents a novel species of the genus Ornithinibacillus, for which the name Ornithinibacillus caprae sp.
32193578	10	52	theme	Ornithinibacillus	1188:1204	arg1	species					1167:1173	a novel species	1159:1173	a novel species	1159:1173	On the basis of the data presented, strain L9T represents a novel species of the genus Ornithinibacillus, for which the name Ornithinibacillus caprae sp.
32193578	4	53	theme	major	332:336	arg1	iso-C15:0					378:386	iso-C15:0	378:386	iso-C15:0	378:386	The predominant menaquinone was MK-7, and the major cellular fatty acids were identified as iso-C15:0 and anteiso-C15:0.
32193578	4	53	theme	major	332:336	arg1	acids					353:357	the major cellular fatty acids	328:357	the major cellular fatty acids	328:357	The predominant menaquinone was MK-7, and the major cellular fatty acids were identified as iso-C15:0 and anteiso-C15:0.
32193578	4	53	theme	major	332:336	arg1	anteiso-C15:0					392:404	anteiso-C15:0	392:404	anteiso-C15:0	392:404	The predominant menaquinone was MK-7, and the major cellular fatty acids were identified as iso-C15:0 and anteiso-C15:0.
32193578	5	54	theme	amino	425:429	arg1	type					436:439	The peptidoglycan amino acid type	407:439	The peptidoglycan amino acid type	407:439	The peptidoglycan amino acid type was determined to be A4β, containing L-ornithine and D-aspartic as diagnostic amino acids.
32193578	5	54	theme	amino	425:429	arg1	A4β					462:464	A4β	462:464	A4β	462:464	The peptidoglycan amino acid type was determined to be A4β, containing L-ornithine and D-aspartic as diagnostic amino acids.
32193578	7	55	theme	35.9 mol	767:774	arg1	%					775:775	35.9 mol%	767:775	35.9 mol%	767:775	Genome sequencing resulted in a genome size of 4.0 Mbp and a DNA G + C content of 35.9 mol%.
32193578	10	56	dep	data	1121:1124	arg1	the					1104:1106	the	1104:1106	the	1104:1106	On the basis of the data presented, strain L9T represents a novel species of the genus Ornithinibacillus, for which the name Ornithinibacillus caprae sp.
32193578	10	56	dep	data	1121:1124	arg1	basis					1108:1112	basis	1108:1112	basis	1108:1112	On the basis of the data presented, strain L9T represents a novel species of the genus Ornithinibacillus, for which the name Ornithinibacillus caprae sp.
32193578	8	57	theme	16S	810:812	arg1	sequences					824:832	the 16S rRNA gene sequences	806:832	the 16S rRNA gene sequences	806:832	Phylogenetic trees based on the 16S rRNA gene sequences showed the isolate to be closely related to Oceanobacillus limi H9BT (98.2% similarity) and Ornithinibacillus halophilus G8BT (97.5% similarity).
32193578	5	58	theme	acid	431:434	arg1	type					436:439	The peptidoglycan amino acid type	407:439	The peptidoglycan amino acid type	407:439	The peptidoglycan amino acid type was determined to be A4β, containing L-ornithine and D-aspartic as diagnostic amino acids.
32193578	5	58	theme	acid	431:434	arg1	A4β					462:464	A4β	462:464	A4β	462:464	The peptidoglycan amino acid type was determined to be A4β, containing L-ornithine and D-aspartic as diagnostic amino acids.
32193578	8	59	theme	rRNA	814:817	arg1	sequences					824:832	the 16S rRNA gene sequences	806:832	the 16S rRNA gene sequences	806:832	Phylogenetic trees based on the 16S rRNA gene sequences showed the isolate to be closely related to Oceanobacillus limi H9BT (98.2% similarity) and Ornithinibacillus halophilus G8BT (97.5% similarity).
32193578	2	60	theme	novel	99:103	arg1	L9T					161:163	strain L9T	154:163	strain L9T	154:163	A novel Gram-stain-positive, motile, moderate halophile, strain L9T, was isolated from hides of white goat in China.
32193578	2	60	theme	novel	99:103	arg1	motile					126:131	motile	126:131	motile	126:131	A novel Gram-stain-positive, motile, moderate halophile, strain L9T, was isolated from hides of white goat in China.
32193578	2	60	theme	novel	99:103	arg1	halophile					143:151	moderate halophile	134:151	moderate halophile	134:151	A novel Gram-stain-positive, motile, moderate halophile, strain L9T, was isolated from hides of white goat in China.
32193578	2	60	theme	novel	99:103	arg1	Gram-stain-positive					105:123	A novel Gram-stain-positive	97:123	A novel Gram-stain-positive	97:123	A novel Gram-stain-positive, motile, moderate halophile, strain L9T, was isolated from hides of white goat in China.
32193578	2	61	theme	goat	199:202	arg1	hides					184:188	hides	184:188	hides of white goat in China	184:211	A novel Gram-stain-positive, motile, moderate halophile, strain L9T, was isolated from hides of white goat in China.
32193578	8	62	theme	Oceanobacillus	878:891	arg1	similarity					910:919	98.2% similarity	904:919	98.2% similarity	904:919	Phylogenetic trees based on the 16S rRNA gene sequences showed the isolate to be closely related to Oceanobacillus limi H9BT (98.2% similarity) and Ornithinibacillus halophilus G8BT (97.5% similarity).
32193578	8	62	theme	Oceanobacillus	878:891	arg1	H9BT					898:901	Oceanobacillus limi H9BT	878:901	Oceanobacillus limi H9BT (98.2% similarity)	878:920	Phylogenetic trees based on the 16S rRNA gene sequences showed the isolate to be closely related to Oceanobacillus limi H9BT (98.2% similarity) and Ornithinibacillus halophilus G8BT (97.5% similarity).
32193578	10	63	theme	strain	1137:1142	arg1	L9T					1144:1146	strain L9T	1137:1146	strain L9T	1137:1146	On the basis of the data presented, strain L9T represents a novel species of the genus Ornithinibacillus, for which the name Ornithinibacillus caprae sp.
34085683	4	0	theme	high	886:889	arg1	capacity					903:910	their high antioxidant capacity	880:910	their high antioxidant capacity	880:910	Moreover, these edible insect flours exhibited effective hyperglycaemia and hyperlipidaemia properties, which together with their high antioxidant capacity are associated with beneficial in vitro physiological effects.
34085683	3	1	theme	average	715:721	arg1	values					723:728	average values	715:728	average values	715:728	The techno-functional properties showed high oil holding, swelling, and emulsifying capacities in all the analysed insect flours, whereas their bulk density, hydration properties, and foaming capacity showed average values and no gelation capacity.
34085683	1	2	theme	flours	256:261	arg1	properties					242:251	and the techno-functional and in vitro physiological properties	189:251	properties	242:251	This work is aimed to evaluate the nutritional composition, and the techno-functional and in vitro physiological properties of flours made using six different insect species and the sensorial feasibility of including them in bakery products.
34085683	1	2	theme	flours	256:261	arg1	feasibility					321:331	the sensorial feasibility	307:331	the sensorial feasibility of including them in bakery products	307:368	This work is aimed to evaluate the nutritional composition, and the techno-functional and in vitro physiological properties of flours made using six different insect species and the sensorial feasibility of including them in bakery products.
34085683	1	2	theme	flours	256:261	arg1	composition					176:186	the nutritional composition	160:186	the nutritional composition	160:186	This work is aimed to evaluate the nutritional composition, and the techno-functional and in vitro physiological properties of flours made using six different insect species and the sensorial feasibility of including them in bakery products.
34085683	2	3	theme	chitin	484:489	arg1	presence					472:479	the presence	468:479	the presence of chitin in ant samples	468:504	The insect flours exhibited high protein and fat contents as their main components, highlighting the presence of chitin in ant samples.
34085683	7	4	theme	food	1412:1415	arg1	properties					1417:1426	functional and sensory food properties	1389:1426	functional and sensory food properties	1389:1426	Thus, these insect flours may be of great interest to the food industry as a healthy source of protein, exerting a positive impact on functional and sensory food properties, and with a potential role in the prevention of diseases associated with hyperglycaemia and hyperlipidaemia.
34085683	2	5	attach	presence	472:479	arg2	chitin					484:489	chitin	484:489	chitin	484:489	The insect flours exhibited high protein and fat contents as their main components, highlighting the presence of chitin in ant samples.
34085683	2	5	attach	presence	472:479	arg1	samples					498:504	ant samples	494:504	ant samples	494:504	The insect flours exhibited high protein and fat contents as their main components, highlighting the presence of chitin in ant samples.
34085683	4	6	theme	insect	779:784	arg1	flours					786:791	these edible insect flours	766:791	these edible insect flours	766:791	Moreover, these edible insect flours exhibited effective hyperglycaemia and hyperlipidaemia properties, which together with their high antioxidant capacity are associated with beneficial in vitro physiological effects.
34085683	1	7	theme	bakery	354:359	arg1	products					361:368	bakery products	354:368	bakery products	354:368	This work is aimed to evaluate the nutritional composition, and the techno-functional and in vitro physiological properties of flours made using six different insect species and the sensorial feasibility of including them in bakery products.
34085683	3	8	theme	techno-functional	511:527	arg1	properties					529:538	The techno-functional properties	507:538	The techno-functional properties	507:538	The techno-functional properties showed high oil holding, swelling, and emulsifying capacities in all the analysed insect flours, whereas their bulk density, hydration properties, and foaming capacity showed average values and no gelation capacity.
34085683	3	9	theme	oil	552:554	arg1	holding					556:562	high oil holding	547:562	high oil holding	547:562	The techno-functional properties showed high oil holding, swelling, and emulsifying capacities in all the analysed insect flours, whereas their bulk density, hydration properties, and foaming capacity showed average values and no gelation capacity.
34085683	0	10	theme	techno-functional	81:97	arg1	properties					117:126	techno-functional and physiological properties	81:126	techno-functional and physiological properties	81:126	Investigating edible insects as a sustainable food source: nutritional value and techno-functional and physiological properties.
34085683	7	11	theme	potential	1440:1448	arg1	role					1450:1453	a potential role	1438:1453	a potential role in the prevention of diseases associated with hyperglycaemia and hyperlipidaemia	1438:1534	Thus, these insect flours may be of great interest to the food industry as a healthy source of protein, exerting a positive impact on functional and sensory food properties, and with a potential role in the prevention of diseases associated with hyperglycaemia and hyperlipidaemia.
34085683	4	12	theme	antioxidant	891:901	arg1	capacity					903:910	their high antioxidant capacity	880:910	their high antioxidant capacity	880:910	Moreover, these edible insect flours exhibited effective hyperglycaemia and hyperlipidaemia properties, which together with their high antioxidant capacity are associated with beneficial in vitro physiological effects.
34085683	7	13	theme	insect	1267:1272	arg1	flours					1274:1279	these insect flours	1261:1279	these insect flours	1261:1279	Thus, these insect flours may be of great interest to the food industry as a healthy source of protein, exerting a positive impact on functional and sensory food properties, and with a potential role in the prevention of diseases associated with hyperglycaemia and hyperlipidaemia.
34085683	7	14	theme	food	1313:1316	arg1	industry					1318:1325	the food industry	1309:1325	the food industry	1309:1325	Thus, these insect flours may be of great interest to the food industry as a healthy source of protein, exerting a positive impact on functional and sensory food properties, and with a potential role in the prevention of diseases associated with hyperglycaemia and hyperlipidaemia.
34085683	4	15	theme	edible	772:777	arg1	flours					786:791	these edible insect flours	766:791	these edible insect flours	766:791	Moreover, these edible insect flours exhibited effective hyperglycaemia and hyperlipidaemia properties, which together with their high antioxidant capacity are associated with beneficial in vitro physiological effects.
34085683	1	16	theme	nutritional	164:174	arg1	composition					176:186	the nutritional composition	160:186	the nutritional composition	160:186	This work is aimed to evaluate the nutritional composition, and the techno-functional and in vitro physiological properties of flours made using six different insect species and the sensorial feasibility of including them in bakery products.
34085683	0	17	theme	physiological	103:115	arg1	properties					117:126	techno-functional and physiological properties	81:126	techno-functional and physiological properties	81:126	Investigating edible insects as a sustainable food source: nutritional value and techno-functional and physiological properties.
34085683	7	18	with	impact	1379:1384	arg1	role					1450:1453	a potential role	1438:1453	a potential role in the prevention of diseases associated with hyperglycaemia and hyperlipidaemia	1438:1534	Thus, these insect flours may be of great interest to the food industry as a healthy source of protein, exerting a positive impact on functional and sensory food properties, and with a potential role in the prevention of diseases associated with hyperglycaemia and hyperlipidaemia.
34085683	3	19	theme	high	547:550	arg1	holding					556:562	high oil holding	547:562	high oil holding	547:562	The techno-functional properties showed high oil holding, swelling, and emulsifying capacities in all the analysed insect flours, whereas their bulk density, hydration properties, and foaming capacity showed average values and no gelation capacity.
34085683	2	20	theme	high	399:402	arg1	protein					404:410	high protein	399:410	high protein	399:410	The insect flours exhibited high protein and fat contents as their main components, highlighting the presence of chitin in ant samples.
34085683	0	21	theme	edible	14:19	arg1	insects					21:27	edible insects	14:27	edible insects	14:27	Investigating edible insects as a sustainable food source: nutritional value and techno-functional and physiological properties.
34085683	0	21	theme	edible	14:19	arg1	source					51:56	a sustainable food source	32:56	a sustainable food source	32:56	Investigating edible insects as a sustainable food source: nutritional value and techno-functional and physiological properties.
34085683	2	22	from	presence	472:479	arg1	samples					498:504	ant samples	494:504	ant samples	494:504	The insect flours exhibited high protein and fat contents as their main components, highlighting the presence of chitin in ant samples.
34085683	6	23	theme	baked	1177:1181	arg1	goods					1183:1187	baked goods	1177:1187	baked goods	1177:1187	The sensory evaluation confirmed that the edible beetle powder can be successfully included in baked goods to provide excellent sensory properties and very high acceptance.
34085683	1	24	theme	different	278:286	arg1	species					295:301	six different insect species	274:301	six different insect species	274:301	This work is aimed to evaluate the nutritional composition, and the techno-functional and in vitro physiological properties of flours made using six different insect species and the sensorial feasibility of including them in bakery products.
34085683	7	25	theme	sensory	1404:1410	arg1	properties					1417:1426	functional and sensory food properties	1389:1426	functional and sensory food properties	1389:1426	Thus, these insect flours may be of great interest to the food industry as a healthy source of protein, exerting a positive impact on functional and sensory food properties, and with a potential role in the prevention of diseases associated with hyperglycaemia and hyperlipidaemia.
34085683	2	26	theme	main	438:441	arg1	components					443:452	their main components	432:452	their main components	432:452	The insect flours exhibited high protein and fat contents as their main components, highlighting the presence of chitin in ant samples.
34085683	1	27	theme	insect	288:293	arg1	species					295:301	six different insect species	274:301	six different insect species	274:301	This work is aimed to evaluate the nutritional composition, and the techno-functional and in vitro physiological properties of flours made using six different insect species and the sensorial feasibility of including them in bakery products.
34085683	6	28	theme	high	1238:1241	arg1	acceptance					1243:1252	very high acceptance	1233:1252	very high acceptance	1233:1252	The sensory evaluation confirmed that the edible beetle powder can be successfully included in baked goods to provide excellent sensory properties and very high acceptance.
34085683	5	29	theme	beetle	979:984	arg1	flours					1002:1007	The beetle and caterpillar flours	975:1007	The beetle and caterpillar flours	975:1007	The beetle and caterpillar flours stand out in these properties, and thus were selected to make a cupcake.
34085683	5	30	theme	caterpillar	990:1000	arg1	flours					1002:1007	The beetle and caterpillar flours	975:1007	The beetle and caterpillar flours	975:1007	The beetle and caterpillar flours stand out in these properties, and thus were selected to make a cupcake.
34085683	7	31	from	role	1450:1453	arg1	prevention					1462:1471	the prevention	1458:1471	the prevention of diseases associated with hyperglycaemia and hyperlipidaemia	1458:1534	Thus, these insect flours may be of great interest to the food industry as a healthy source of protein, exerting a positive impact on functional and sensory food properties, and with a potential role in the prevention of diseases associated with hyperglycaemia and hyperlipidaemia.
34085683	3	32	theme	no	734:735	arg1	capacity					746:753	no gelation capacity	734:753	no gelation capacity	734:753	The techno-functional properties showed high oil holding, swelling, and emulsifying capacities in all the analysed insect flours, whereas their bulk density, hydration properties, and foaming capacity showed average values and no gelation capacity.
34085683	4	33	theme	in	943:944	arg1	effects					966:972	beneficial in vitro physiological effects	932:972	beneficial in vitro physiological effects	932:972	Moreover, these edible insect flours exhibited effective hyperglycaemia and hyperlipidaemia properties, which together with their high antioxidant capacity are associated with beneficial in vitro physiological effects.
34085683	6	34	theme	excellent	1200:1208	arg1	properties					1218:1227	excellent sensory properties	1200:1227	excellent sensory properties	1200:1227	The sensory evaluation confirmed that the edible beetle powder can be successfully included in baked goods to provide excellent sensory properties and very high acceptance.
34085683	7	35	theme	functional	1389:1398	arg1	properties					1417:1426	functional and sensory food properties	1389:1426	functional and sensory food properties	1389:1426	Thus, these insect flours may be of great interest to the food industry as a healthy source of protein, exerting a positive impact on functional and sensory food properties, and with a potential role in the prevention of diseases associated with hyperglycaemia and hyperlipidaemia.
34085683	1	36	theme	techno-functional	197:213	arg1	properties					242:251	and the techno-functional and in vitro physiological properties	189:251	properties	242:251	This work is aimed to evaluate the nutritional composition, and the techno-functional and in vitro physiological properties of flours made using six different insect species and the sensorial feasibility of including them in bakery products.
34085683	0	37	theme	sustainable	34:44	arg1	insects					21:27	edible insects	14:27	edible insects	14:27	Investigating edible insects as a sustainable food source: nutritional value and techno-functional and physiological properties.
34085683	0	37	theme	sustainable	34:44	arg1	source					51:56	a sustainable food source	32:56	a sustainable food source	32:56	Investigating edible insects as a sustainable food source: nutritional value and techno-functional and physiological properties.
34085683	3	38	theme	gelation	737:744	arg1	capacity					746:753	no gelation capacity	734:753	no gelation capacity	734:753	The techno-functional properties showed high oil holding, swelling, and emulsifying capacities in all the analysed insect flours, whereas their bulk density, hydration properties, and foaming capacity showed average values and no gelation capacity.
34085683	3	39	theme	bulk	651:654	arg1	density					656:662	their bulk density	645:662	their bulk density	645:662	The techno-functional properties showed high oil holding, swelling, and emulsifying capacities in all the analysed insect flours, whereas their bulk density, hydration properties, and foaming capacity showed average values and no gelation capacity.
34085683	7	40	theme	positive	1370:1377	arg1	impact					1379:1384	a positive impact	1368:1384	a positive impact on functional and sensory food properties, and with a potential role in the prevention of diseases associated with hyperglycaemia and hyperlipidaemia	1368:1534	Thus, these insect flours may be of great interest to the food industry as a healthy source of protein, exerting a positive impact on functional and sensory food properties, and with a potential role in the prevention of diseases associated with hyperglycaemia and hyperlipidaemia.
34085683	6	41	theme	sensory	1210:1216	arg1	properties					1218:1227	excellent sensory properties	1200:1227	excellent sensory properties	1200:1227	The sensory evaluation confirmed that the edible beetle powder can be successfully included in baked goods to provide excellent sensory properties and very high acceptance.
34085683	2	42	theme	fat	416:418	arg1	contents					420:427	high protein and fat contents	399:427	high protein and fat contents as their main components	399:452	The insect flours exhibited high protein and fat contents as their main components, highlighting the presence of chitin in ant samples.
34085683	2	43	theme	protein	404:410	arg1	contents					420:427	high protein and fat contents	399:427	high protein and fat contents as their main components	399:452	The insect flours exhibited high protein and fat contents as their main components, highlighting the presence of chitin in ant samples.
34085683	4	44	theme	physiological	952:964	arg1	effects					966:972	beneficial in vitro physiological effects	932:972	beneficial in vitro physiological effects	932:972	Moreover, these edible insect flours exhibited effective hyperglycaemia and hyperlipidaemia properties, which together with their high antioxidant capacity are associated with beneficial in vitro physiological effects.
34085683	4	45	theme	hyperglycaemia	813:826	arg1	properties					848:857	effective hyperglycaemia and hyperlipidaemia properties	803:857	effective hyperglycaemia and hyperlipidaemia properties	803:857	Moreover, these edible insect flours exhibited effective hyperglycaemia and hyperlipidaemia properties, which together with their high antioxidant capacity are associated with beneficial in vitro physiological effects.
34085683	1	46	theme	in	219:220	arg1	properties					242:251	and the techno-functional and in vitro physiological properties	189:251	properties	242:251	This work is aimed to evaluate the nutritional composition, and the techno-functional and in vitro physiological properties of flours made using six different insect species and the sensorial feasibility of including them in bakery products.
34085683	1	47	theme	sensorial	311:319	arg1	feasibility					321:331	the sensorial feasibility	307:331	the sensorial feasibility of including them in bakery products	307:368	This work is aimed to evaluate the nutritional composition, and the techno-functional and in vitro physiological properties of flours made using six different insect species and the sensorial feasibility of including them in bakery products.
34085683	3	48	theme	emulsifying	579:589	arg1	capacities					591:600	emulsifying capacities	579:600	emulsifying capacities	579:600	The techno-functional properties showed high oil holding, swelling, and emulsifying capacities in all the analysed insect flours, whereas their bulk density, hydration properties, and foaming capacity showed average values and no gelation capacity.
34085683	6	49	theme	edible	1124:1129	arg1	powder					1138:1143	the edible beetle powder	1120:1143	the edible beetle powder	1120:1143	The sensory evaluation confirmed that the edible beetle powder can be successfully included in baked goods to provide excellent sensory properties and very high acceptance.
34085683	3	50	dep	showed	540:545	arg1	whereas					637:643	whereas	637:643	whereas	637:643	The techno-functional properties showed high oil holding, swelling, and emulsifying capacities in all the analysed insect flours, whereas their bulk density, hydration properties, and foaming capacity showed average values and no gelation capacity.
34085683	0	51	theme	food	46:49	arg1	insects					21:27	edible insects	14:27	edible insects	14:27	Investigating edible insects as a sustainable food source: nutritional value and techno-functional and physiological properties.
34085683	0	51	theme	food	46:49	arg1	source					51:56	a sustainable food source	32:56	a sustainable food source	32:56	Investigating edible insects as a sustainable food source: nutritional value and techno-functional and physiological properties.
34085683	3	52	theme	hydration	665:673	arg1	properties					675:684	hydration properties	665:684	hydration properties	665:684	The techno-functional properties showed high oil holding, swelling, and emulsifying capacities in all the analysed insect flours, whereas their bulk density, hydration properties, and foaming capacity showed average values and no gelation capacity.
34085683	4	53	theme	hyperlipidaemia	832:846	arg1	properties					848:857	effective hyperglycaemia and hyperlipidaemia properties	803:857	effective hyperglycaemia and hyperlipidaemia properties	803:857	Moreover, these edible insect flours exhibited effective hyperglycaemia and hyperlipidaemia properties, which together with their high antioxidant capacity are associated with beneficial in vitro physiological effects.
34085683	7	54	theme	healthy	1332:1338	arg1	source					1340:1345	a healthy source	1330:1345	a healthy source of protein	1330:1356	Thus, these insect flours may be of great interest to the food industry as a healthy source of protein, exerting a positive impact on functional and sensory food properties, and with a potential role in the prevention of diseases associated with hyperglycaemia and hyperlipidaemia.
34085683	7	55	theme	great	1291:1295	arg1	interest					1297:1304	great interest	1291:1304	great interest	1291:1304	Thus, these insect flours may be of great interest to the food industry as a healthy source of protein, exerting a positive impact on functional and sensory food properties, and with a potential role in the prevention of diseases associated with hyperglycaemia and hyperlipidaemia.
34085683	3	56	theme	analysed	613:620	arg1	flours					629:634	all the analysed insect flours	605:634	all the analysed insect flours	605:634	The techno-functional properties showed high oil holding, swelling, and emulsifying capacities in all the analysed insect flours, whereas their bulk density, hydration properties, and foaming capacity showed average values and no gelation capacity.
34085683	4	57	theme	beneficial	932:941	arg1	effects					966:972	beneficial in vitro physiological effects	932:972	beneficial in vitro physiological effects	932:972	Moreover, these edible insect flours exhibited effective hyperglycaemia and hyperlipidaemia properties, which together with their high antioxidant capacity are associated with beneficial in vitro physiological effects.
34085683	7	58	theme	protein	1350:1356	arg1	source					1340:1345	a healthy source	1330:1345	a healthy source of protein	1330:1356	Thus, these insect flours may be of great interest to the food industry as a healthy source of protein, exerting a positive impact on functional and sensory food properties, and with a potential role in the prevention of diseases associated with hyperglycaemia and hyperlipidaemia.
34085683	3	59	theme	insect	622:627	arg1	flours					629:634	all the analysed insect flours	605:634	all the analysed insect flours	605:634	The techno-functional properties showed high oil holding, swelling, and emulsifying capacities in all the analysed insect flours, whereas their bulk density, hydration properties, and foaming capacity showed average values and no gelation capacity.
34085683	4	60	dep	in	943:944	arg1	vitro					946:950	vitro	946:950	vitro	946:950	Moreover, these edible insect flours exhibited effective hyperglycaemia and hyperlipidaemia properties, which together with their high antioxidant capacity are associated with beneficial in vitro physiological effects.
34085683	7	61	from	impact	1379:1384	arg1	properties					1417:1426	functional and sensory food properties	1389:1426	functional and sensory food properties	1389:1426	Thus, these insect flours may be of great interest to the food industry as a healthy source of protein, exerting a positive impact on functional and sensory food properties, and with a potential role in the prevention of diseases associated with hyperglycaemia and hyperlipidaemia.
34085683	7	62	theme	diseases	1476:1483	arg1	prevention					1462:1471	the prevention	1458:1471	the prevention of diseases associated with hyperglycaemia and hyperlipidaemia	1458:1534	Thus, these insect flours may be of great interest to the food industry as a healthy source of protein, exerting a positive impact on functional and sensory food properties, and with a potential role in the prevention of diseases associated with hyperglycaemia and hyperlipidaemia.
34085683	1	63	dep	in	219:220	arg1	vitro					222:226	vitro	222:226	vitro	222:226	This work is aimed to evaluate the nutritional composition, and the techno-functional and in vitro physiological properties of flours made using six different insect species and the sensorial feasibility of including them in bakery products.
34085683	6	64	theme	beetle	1131:1136	arg1	powder					1138:1143	the edible beetle powder	1120:1143	the edible beetle powder	1120:1143	The sensory evaluation confirmed that the edible beetle powder can be successfully included in baked goods to provide excellent sensory properties and very high acceptance.
34085683	1	65	theme	physiological	228:240	arg1	properties					242:251	and the techno-functional and in vitro physiological properties	189:251	properties	242:251	This work is aimed to evaluate the nutritional composition, and the techno-functional and in vitro physiological properties of flours made using six different insect species and the sensorial feasibility of including them in bakery products.
34085683	0	66	theme	nutritional	59:69	arg1	value					71:75	nutritional value	59:75	nutritional value	59:75	Investigating edible insects as a sustainable food source: nutritional value and techno-functional and physiological properties.
34085683	4	67	theme	effective	803:811	arg1	hyperglycaemia					813:826	effective hyperglycaemia	803:826	effective hyperglycaemia	803:826	Moreover, these edible insect flours exhibited effective hyperglycaemia and hyperlipidaemia properties, which together with their high antioxidant capacity are associated with beneficial in vitro physiological effects.
34085683	2	68	theme	insect	375:380	arg1	flours					382:387	The insect flours	371:387	The insect flours	371:387	The insect flours exhibited high protein and fat contents as their main components, highlighting the presence of chitin in ant samples.
34085683	6	69	theme	sensory	1086:1092	arg1	evaluation					1094:1103	The sensory evaluation	1082:1103	The sensory evaluation	1082:1103	The sensory evaluation confirmed that the edible beetle powder can be successfully included in baked goods to provide excellent sensory properties and very high acceptance.
34085683	2	70	theme	ant	494:496	arg1	samples					498:504	ant samples	494:504	ant samples	494:504	The insect flours exhibited high protein and fat contents as their main components, highlighting the presence of chitin in ant samples.
34085683	3	71	theme	foaming	691:697	arg1	capacity					699:706	foaming capacity	691:706	foaming capacity	691:706	The techno-functional properties showed high oil holding, swelling, and emulsifying capacities in all the analysed insect flours, whereas their bulk density, hydration properties, and foaming capacity showed average values and no gelation capacity.
34085683	0	72	dep	Investigating	0:12	arg1	value					71:75	nutritional value	59:75	nutritional value	59:75	Investigating edible insects as a sustainable food source: nutritional value and techno-functional and physiological properties.
34085683	0	72	dep	Investigating	0:12	arg1	properties					117:126	techno-functional and physiological properties	81:126	techno-functional and physiological properties	81:126	Investigating edible insects as a sustainable food source: nutritional value and techno-functional and physiological properties.
33078401	7	0	theme	biological	1346:1355	arg1	activities					1357:1366	the biological activities	1342:1366	the biological activities of dietary fibers	1342:1384	A large amount of bound phenolic compounds in dietary fibers can add to the biological activities of dietary fibers.
33078401	3	1	dep	spectra	584:590	arg1	contain					626:632	contain	626:632	may contain cellulose	622:642	It can be inferred from the infrared and UV-Vis spectra that the SDF and IDF of algae may contain cellulose, hemicellulose, various monosaccharides, phenolic compounds, and quinone pigments.
33078401	6	2	dep	APPLICATION	1152:1162	arg1	digested					1216:1223	digested	1216:1223	cannot be digested by humans, hence regarded as dietary fibers	1206:1267	PRACTICAL APPLICATION: Polysaccharides of high content in algae cannot be digested by humans, hence regarded as dietary fibers.
33078401	2	3	theme	fiber	528:532	arg1	%					513:513	39.10 to 93.52%	499:513	39.10 to 93.52% of the total fiber	499:532	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae were significantly lower than the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber).
33078401	2	3	theme	fiber	528:532	arg1	fiber					528:532	the total fiber	518:532	the total fiber	518:532	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae were significantly lower than the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber).
33078401	7	4	theme	phenolic	1294:1301	arg1	compounds					1303:1311	bound phenolic compounds	1288:1311	bound phenolic compounds	1288:1311	A large amount of bound phenolic compounds in dietary fibers can add to the biological activities of dietary fibers.
33078401	0	5	from	algae	160:164	arg1	compounds					135:143	bound phenolic compounds	120:143	bound phenolic compounds from different algae	120:164	Comparative analysis of the morphological property and chemical composition of soluble and insoluble dietary fiber with bound phenolic compounds from different algae.
33078401	6	6	theme	PRACTICAL	1142:1150	arg1	APPLICATION					1152:1162	PRACTICAL APPLICATION	1142:1162	PRACTICAL APPLICATION: Polysaccharides of high content in algae cannot be digested by humans, hence regarded as dietary fibers.	1142:1268	PRACTICAL APPLICATION: Polysaccharides of high content in algae cannot be digested by humans, hence regarded as dietary fibers.
33078401	3	7	theme	algae	616:620	arg1	IDF					609:611	IDF	609:611	IDF	609:611	It can be inferred from the infrared and UV-Vis spectra that the SDF and IDF of algae may contain cellulose, hemicellulose, various monosaccharides, phenolic compounds, and quinone pigments.
33078401	3	7	theme	algae	616:620	arg1	SDF					601:603	SDF	601:603	SDF	601:603	It can be inferred from the infrared and UV-Vis spectra that the SDF and IDF of algae may contain cellulose, hemicellulose, various monosaccharides, phenolic compounds, and quinone pigments.
33078401	7	8	theme	dietary	1316:1322	arg1	fibers					1324:1329	dietary fibers	1316:1329	dietary fibers	1316:1329	A large amount of bound phenolic compounds in dietary fibers can add to the biological activities of dietary fibers.
33078401	2	9	from	contents	346:353	arg1	most					394:397	most	394:397	most	394:397	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae were significantly lower than the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber).
33078401	2	9	from	contents	346:353	arg1	algae					419:423	the investigated algae	402:423	the investigated algae	402:423	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae were significantly lower than the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber).
33078401	7	10	theme	large	1272:1276	arg1	amount					1278:1283	A large amount	1270:1283	A large amount of bound phenolic compounds in dietary fibers	1270:1329	A large amount of bound phenolic compounds in dietary fibers can add to the biological activities of dietary fibers.
33078401	7	10	theme	large	1272:1276	arg1	compounds					1303:1311	bound phenolic compounds	1288:1311	bound phenolic compounds	1288:1311	A large amount of bound phenolic compounds in dietary fibers can add to the biological activities of dietary fibers.
33078401	0	11	theme	different	150:158	arg1	algae					160:164	different algae	150:164	different algae	150:164	Comparative analysis of the morphological property and chemical composition of soluble and insoluble dietary fiber with bound phenolic compounds from different algae.
33078401	2	12	theme	soluble	318:324	arg1	contents					346:353	The soluble dietary fiber (SDF) contents	314:353	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae	314:423	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae were significantly lower than the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber).
33078401	2	12	theme	soluble	318:324	arg1	lower					444:448	lower	444:448	lower	444:448	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae were significantly lower than the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber).
33078401	5	13	contain	contain	1040:1046	arg1	IDF					1032:1034	the IDF	1028:1034	the IDF	1028:1034	The HPLC-mass spectrometry (MS)/MS results showed that the IDF may contain methyl-8α-hydroxy-grindelate-7β-O-7'β-ether hydrate, hydroxydecanoic acid, and malyngic acid.
33078401	5	13	contain	contain	1040:1046	arg2	acid					1117:1120	hydroxydecanoic acid	1101:1120	hydroxydecanoic acid	1101:1120	The HPLC-mass spectrometry (MS)/MS results showed that the IDF may contain methyl-8α-hydroxy-grindelate-7β-O-7'β-ether hydrate, hydroxydecanoic acid, and malyngic acid.
33078401	5	13	contain	contain	1040:1046	arg2	hydrate					1092:1098	methyl-8α-hydroxy-grindelate-7β-O-7'β-ether hydrate	1048:1098	methyl-8α-hydroxy-grindelate-7β-O-7'β-ether hydrate	1048:1098	The HPLC-mass spectrometry (MS)/MS results showed that the IDF may contain methyl-8α-hydroxy-grindelate-7β-O-7'β-ether hydrate, hydroxydecanoic acid, and malyngic acid.
33078401	5	13	contain	contain	1040:1046	arg2	acid					1136:1139	malyngic acid	1127:1139	malyngic acid	1127:1139	The HPLC-mass spectrometry (MS)/MS results showed that the IDF may contain methyl-8α-hydroxy-grindelate-7β-O-7'β-ether hydrate, hydroxydecanoic acid, and malyngic acid.
33078401	0	14	theme	bound	120:124	arg1	compounds					135:143	bound phenolic compounds	120:143	bound phenolic compounds from different algae	120:164	Comparative analysis of the morphological property and chemical composition of soluble and insoluble dietary fiber with bound phenolic compounds from different algae.
33078401	1	15	theme	biochemical	207:217	arg1	properties					219:228	The morphological, physicochemical, and biochemical properties	167:228	The morphological, physicochemical, and biochemical properties of soluble and insoluble dietary fiber from seven types of algae	167:293	The morphological, physicochemical, and biochemical properties of soluble and insoluble dietary fiber from seven types of algae were investigated.
33078401	3	16	theme	phenolic	685:692	arg1	compounds					694:702	phenolic compounds	685:702	phenolic compounds	685:702	It can be inferred from the infrared and UV-Vis spectra that the SDF and IDF of algae may contain cellulose, hemicellulose, various monosaccharides, phenolic compounds, and quinone pigments.
33078401	4	17	from	phenolic	737:744	arg1	algae					759:763	the seven algae	749:763	the seven algae	749:763	The bound phenolic in the seven algae varied widely in contents (3.76 to 14.08 mg GAE/g in IDF and 1.94 to 8.61 mg GAE/g in SDF), whose antioxidant activities in the IDF were stronger than those in SDF because of different phenolic compositions.
33078401	1	18	theme	algae	289:293	arg1	types					280:284	seven types	274:284	seven types of algae	274:293	The morphological, physicochemical, and biochemical properties of soluble and insoluble dietary fiber from seven types of algae were investigated.
33078401	6	19	theme	content	1189:1195	arg1	Polysaccharides					1165:1179	Polysaccharides	1165:1179	Polysaccharides of high content in algae	1165:1204	PRACTICAL APPLICATION: Polysaccharides of high content in algae cannot be digested by humans, hence regarded as dietary fibers.
33078401	3	20	theme	quinone	709:715	arg1	pigments					717:724	quinone pigments	709:724	quinone pigments	709:724	It can be inferred from the infrared and UV-Vis spectra that the SDF and IDF of algae may contain cellulose, hemicellulose, various monosaccharides, phenolic compounds, and quinone pigments.
33078401	2	21	theme	dietary	469:475	arg1	contents					489:496	the insoluble dietary fiber (IDF) contents	455:496	the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber)	455:533	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae were significantly lower than the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber).
33078401	6	22	theme	dietary	1254:1260	arg1	fibers					1262:1267	dietary fibers	1254:1267	dietary fibers	1254:1267	PRACTICAL APPLICATION: Polysaccharides of high content in algae cannot be digested by humans, hence regarded as dietary fibers.
33078401	5	23	theme	spectrometry	987:998	arg1	results					1008:1014	The HPLC-mass spectrometry (MS)/MS results	973:1014	The HPLC-mass spectrometry (MS)/MS results	973:1014	The HPLC-mass spectrometry (MS)/MS results showed that the IDF may contain methyl-8α-hydroxy-grindelate-7β-O-7'β-ether hydrate, hydroxydecanoic acid, and malyngic acid.
33078401	5	24	theme	malyngic	1127:1134	arg1	acid					1136:1139	malyngic acid	1127:1139	malyngic acid	1127:1139	The HPLC-mass spectrometry (MS)/MS results showed that the IDF may contain methyl-8α-hydroxy-grindelate-7β-O-7'β-ether hydrate, hydroxydecanoic acid, and malyngic acid.
33078401	0	25	theme	soluble	79:85	arg1	fiber					109:113	soluble and insoluble dietary fiber	79:113	soluble and insoluble dietary fiber	79:113	Comparative analysis of the morphological property and chemical composition of soluble and insoluble dietary fiber with bound phenolic compounds from different algae.
33078401	0	26	theme	insoluble	91:99	arg1	fiber					109:113	soluble and insoluble dietary fiber	79:113	soluble and insoluble dietary fiber	79:113	Comparative analysis of the morphological property and chemical composition of soluble and insoluble dietary fiber with bound phenolic compounds from different algae.
33078401	4	27	from	algae	759:763	arg1	phenolic					737:744	phenolic	737:744	phenolic	737:744	The bound phenolic in the seven algae varied widely in contents (3.76 to 14.08 mg GAE/g in IDF and 1.94 to 8.61 mg GAE/g in SDF), whose antioxidant activities in the IDF were stronger than those in SDF because of different phenolic compositions.
33078401	0	28	theme	fiber	109:113	arg1	property					42:49	morphological property	28:49	morphological property	28:49	Comparative analysis of the morphological property and chemical composition of soluble and insoluble dietary fiber with bound phenolic compounds from different algae.
33078401	0	28	theme	fiber	109:113	arg1	composition					64:74	chemical composition	55:74	chemical composition	55:74	Comparative analysis of the morphological property and chemical composition of soluble and insoluble dietary fiber with bound phenolic compounds from different algae.
33078401	4	29	dep	contents	782:789	arg1	GAE/g					809:813	3.76 to 14.08 mg GAE/g	792:813	3.76 to 14.08 mg GAE/g in IDF and 1.94 to 8.61 mg GAE/g in SDF	792:853	The bound phenolic in the seven algae varied widely in contents (3.76 to 14.08 mg GAE/g in IDF and 1.94 to 8.61 mg GAE/g in SDF), whose antioxidant activities in the IDF were stronger than those in SDF because of different phenolic compositions.
33078401	2	30	theme	investigated	406:417	arg1	algae					419:423	the investigated algae	402:423	the investigated algae	402:423	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae were significantly lower than the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber).
33078401	0	31	theme	Comparative	0:10	arg1	analysis					12:19	Comparative analysis	0:19	Comparative analysis of the morphological property and chemical composition of soluble and insoluble dietary fiber with bound phenolic compounds from different algae.	0:165	Comparative analysis of the morphological property and chemical composition of soluble and insoluble dietary fiber with bound phenolic compounds from different algae.
33078401	3	32	dep	SDF	601:603	arg1	the					597:599	the	597:599	the	597:599	It can be inferred from the infrared and UV-Vis spectra that the SDF and IDF of algae may contain cellulose, hemicellulose, various monosaccharides, phenolic compounds, and quinone pigments.
33078401	4	33	theme	antioxidant	863:873	arg1	activities					875:884	antioxidant activities	863:884	antioxidant activities in the IDF	863:895	The bound phenolic in the seven algae varied widely in contents (3.76 to 14.08 mg GAE/g in IDF and 1.94 to 8.61 mg GAE/g in SDF), whose antioxidant activities in the IDF were stronger than those in SDF because of different phenolic compositions.
33078401	2	34	theme	fiber	384:388	arg1	fiber					384:388	the total fiber	374:388	the total fiber	374:388	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae were significantly lower than the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber).
33078401	2	34	theme	fiber	384:388	arg1	%					369:369	6.48 to 60.90%	356:369	6.48 to 60.90% of the total fiber	356:388	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae were significantly lower than the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber).
33078401	0	35	theme	morphological	28:40	arg1	property					42:49	morphological property	28:49	morphological property	28:49	Comparative analysis of the morphological property and chemical composition of soluble and insoluble dietary fiber with bound phenolic compounds from different algae.
33078401	5	36	theme	/MS	1004:1006	arg1	results					1008:1014	The HPLC-mass spectrometry (MS)/MS results	973:1014	The HPLC-mass spectrometry (MS)/MS results	973:1014	The HPLC-mass spectrometry (MS)/MS results showed that the IDF may contain methyl-8α-hydroxy-grindelate-7β-O-7'β-ether hydrate, hydroxydecanoic acid, and malyngic acid.
33078401	0	37	theme	composition	64:74	arg1	analysis					12:19	Comparative analysis	0:19	Comparative analysis of the morphological property and chemical composition of soluble and insoluble dietary fiber with bound phenolic compounds from different algae.	0:165	Comparative analysis of the morphological property and chemical composition of soluble and insoluble dietary fiber with bound phenolic compounds from different algae.
33078401	7	38	theme	dietary	1371:1377	arg1	fibers					1379:1384	dietary fibers	1371:1384	dietary fibers	1371:1384	A large amount of bound phenolic compounds in dietary fibers can add to the biological activities of dietary fibers.
33078401	8	39	theme	functional	1450:1459	arg1	foods					1461:1465	seaweed-based functional foods	1436:1465	seaweed-based functional foods	1436:1465	These topics are important to the development of seaweed-based functional foods.
33078401	1	40	theme	soluble	233:239	arg1	fiber					263:267	soluble and insoluble dietary fiber	233:267	soluble and insoluble dietary fiber	233:267	The morphological, physicochemical, and biochemical properties of soluble and insoluble dietary fiber from seven types of algae were investigated.
33078401	4	41	theme	phenolic	950:957	arg1	compositions					959:970	different phenolic compositions	940:970	different phenolic compositions	940:970	The bound phenolic in the seven algae varied widely in contents (3.76 to 14.08 mg GAE/g in IDF and 1.94 to 8.61 mg GAE/g in SDF), whose antioxidant activities in the IDF were stronger than those in SDF because of different phenolic compositions.
33078401	2	42	theme	total	378:382	arg1	fiber					384:388	the total fiber	374:388	the total fiber	374:388	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae were significantly lower than the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber).
33078401	6	43	from	Polysaccharides	1165:1179	arg1	algae					1200:1204	algae	1200:1204	algae	1200:1204	PRACTICAL APPLICATION: Polysaccharides of high content in algae cannot be digested by humans, hence regarded as dietary fibers.
33078401	2	44	dep	93.52	508:512	arg1	to					505:506	to	505:506	to	505:506	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae were significantly lower than the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber).
33078401	1	45	theme	fiber	263:267	arg1	properties					219:228	The morphological, physicochemical, and biochemical properties	167:228	The morphological, physicochemical, and biochemical properties of soluble and insoluble dietary fiber from seven types of algae	167:293	The morphological, physicochemical, and biochemical properties of soluble and insoluble dietary fiber from seven types of algae were investigated.
33078401	5	46	theme	β-ether	1084:1090	arg1	hydrate					1092:1098	methyl-8α-hydroxy-grindelate-7β-O-7'β-ether hydrate	1048:1098	methyl-8α-hydroxy-grindelate-7β-O-7'β-ether hydrate	1048:1098	The HPLC-mass spectrometry (MS)/MS results showed that the IDF may contain methyl-8α-hydroxy-grindelate-7β-O-7'β-ether hydrate, hydroxydecanoic acid, and malyngic acid.
33078401	2	47	dep	contents	489:496	arg1	%					513:513	39.10 to 93.52%	499:513	39.10 to 93.52% of the total fiber	499:532	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae were significantly lower than the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber).
33078401	2	47	dep	contents	489:496	arg1	fiber					528:532	the total fiber	518:532	the total fiber	518:532	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae were significantly lower than the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber).
33078401	2	48	theme	total	522:526	arg1	fiber					528:532	the total fiber	518:532	the total fiber	518:532	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae were significantly lower than the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber).
33078401	7	49	theme	compounds	1303:1311	arg1	amount					1278:1283	A large amount	1270:1283	A large amount of bound phenolic compounds in dietary fibers	1270:1329	A large amount of bound phenolic compounds in dietary fibers can add to the biological activities of dietary fibers.
33078401	7	49	theme	compounds	1303:1311	arg1	compounds					1303:1311	bound phenolic compounds	1288:1311	bound phenolic compounds	1288:1311	A large amount of bound phenolic compounds in dietary fibers can add to the biological activities of dietary fibers.
33078401	1	50	theme	morphological	171:183	arg1	properties					219:228	The morphological, physicochemical, and biochemical properties	167:228	The morphological, physicochemical, and biochemical properties of soluble and insoluble dietary fiber from seven types of algae	167:293	The morphological, physicochemical, and biochemical properties of soluble and insoluble dietary fiber from seven types of algae were investigated.
33078401	7	51	theme	bound	1288:1292	arg1	compounds					1303:1311	bound phenolic compounds	1288:1311	bound phenolic compounds	1288:1311	A large amount of bound phenolic compounds in dietary fibers can add to the biological activities of dietary fibers.
33078401	2	52	theme	dietary	326:332	arg1	contents					346:353	The soluble dietary fiber (SDF) contents	314:353	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae	314:423	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae were significantly lower than the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber).
33078401	2	52	theme	dietary	326:332	arg1	lower					444:448	lower	444:448	lower	444:448	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae were significantly lower than the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber).
33078401	1	53	theme	physicochemical	186:200	arg1	properties					219:228	The morphological, physicochemical, and biochemical properties	167:228	The morphological, physicochemical, and biochemical properties of soluble and insoluble dietary fiber from seven types of algae	167:293	The morphological, physicochemical, and biochemical properties of soluble and insoluble dietary fiber from seven types of algae were investigated.
33078401	3	54	theme	various	660:666	arg1	monosaccharides					668:682	various monosaccharides	660:682	various monosaccharides	660:682	It can be inferred from the infrared and UV-Vis spectra that the SDF and IDF of algae may contain cellulose, hemicellulose, various monosaccharides, phenolic compounds, and quinone pigments.
33078401	0	55	theme	phenolic	126:133	arg1	compounds					135:143	bound phenolic compounds	120:143	bound phenolic compounds from different algae	120:164	Comparative analysis of the morphological property and chemical composition of soluble and insoluble dietary fiber with bound phenolic compounds from different algae.
33078401	4	56	from	GAE/g	809:813	arg1	SDF					851:853	SDF	851:853	SDF	851:853	The bound phenolic in the seven algae varied widely in contents (3.76 to 14.08 mg GAE/g in IDF and 1.94 to 8.61 mg GAE/g in SDF), whose antioxidant activities in the IDF were stronger than those in SDF because of different phenolic compositions.
33078401	4	56	from	GAE/g	809:813	arg1	1.94					826:829	1.94	826:829	1.94	826:829	The bound phenolic in the seven algae varied widely in contents (3.76 to 14.08 mg GAE/g in IDF and 1.94 to 8.61 mg GAE/g in SDF), whose antioxidant activities in the IDF were stronger than those in SDF because of different phenolic compositions.
33078401	4	56	from	GAE/g	809:813	arg1	IDF					818:820	IDF	818:820	IDF	818:820	The bound phenolic in the seven algae varied widely in contents (3.76 to 14.08 mg GAE/g in IDF and 1.94 to 8.61 mg GAE/g in SDF), whose antioxidant activities in the IDF were stronger than those in SDF because of different phenolic compositions.
33078401	6	57	theme	high	1184:1187	arg1	content					1189:1195	high content	1184:1195	high content in algae	1184:1204	PRACTICAL APPLICATION: Polysaccharides of high content in algae cannot be digested by humans, hence regarded as dietary fibers.
33078401	3	58	contain	contain	626:632	arg2	cellulose					634:642	cellulose	634:642	cellulose	634:642	It can be inferred from the infrared and UV-Vis spectra that the SDF and IDF of algae may contain cellulose, hemicellulose, various monosaccharides, phenolic compounds, and quinone pigments.
33078401	3	58	contain	contain	626:632	arg1	IDF					609:611	IDF	609:611	IDF	609:611	It can be inferred from the infrared and UV-Vis spectra that the SDF and IDF of algae may contain cellulose, hemicellulose, various monosaccharides, phenolic compounds, and quinone pigments.
33078401	3	58	contain	contain	626:632	arg1	SDF					601:603	SDF	601:603	SDF	601:603	It can be inferred from the infrared and UV-Vis spectra that the SDF and IDF of algae may contain cellulose, hemicellulose, various monosaccharides, phenolic compounds, and quinone pigments.
33078401	4	59	dep	14.08 mg	800:807	arg1	to					797:798	to	797:798	to	797:798	The bound phenolic in the seven algae varied widely in contents (3.76 to 14.08 mg GAE/g in IDF and 1.94 to 8.61 mg GAE/g in SDF), whose antioxidant activities in the IDF were stronger than those in SDF because of different phenolic compositions.
33078401	7	60	from	amount	1278:1283	arg1	fibers					1324:1329	dietary fibers	1316:1329	dietary fibers	1316:1329	A large amount of bound phenolic compounds in dietary fibers can add to the biological activities of dietary fibers.
33078401	2	61	theme	insoluble	459:467	arg1	contents					489:496	the insoluble dietary fiber (IDF) contents	455:496	the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber)	455:533	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae were significantly lower than the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber).
33078401	1	62	from	types	280:284	arg1	properties					219:228	The morphological, physicochemical, and biochemical properties	167:228	The morphological, physicochemical, and biochemical properties of soluble and insoluble dietary fiber from seven types of algae	167:293	The morphological, physicochemical, and biochemical properties of soluble and insoluble dietary fiber from seven types of algae were investigated.
33078401	0	63	theme	dietary	101:107	arg1	fiber					109:113	soluble and insoluble dietary fiber	79:113	soluble and insoluble dietary fiber	79:113	Comparative analysis of the morphological property and chemical composition of soluble and insoluble dietary fiber with bound phenolic compounds from different algae.
33078401	2	64	theme	IDF	484:486	arg1	contents					489:496	the insoluble dietary fiber (IDF) contents	455:496	the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber)	455:533	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae were significantly lower than the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber).
33078401	2	65	dep	contents	346:353	arg1	fiber					384:388	the total fiber	374:388	the total fiber	374:388	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae were significantly lower than the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber).
33078401	2	65	dep	contents	346:353	arg1	%					369:369	6.48 to 60.90%	356:369	6.48 to 60.90% of the total fiber	356:388	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae were significantly lower than the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber).
33078401	4	66	theme	8.61 mg	834:840	arg1	GAE/g					842:846	8.61 mg GAE/g	834:846	8.61 mg GAE/g	834:846	The bound phenolic in the seven algae varied widely in contents (3.76 to 14.08 mg GAE/g in IDF and 1.94 to 8.61 mg GAE/g in SDF), whose antioxidant activities in the IDF were stronger than those in SDF because of different phenolic compositions.
33078401	5	67	theme	HPLC-mass	977:985	arg1	MS					1001:1002	MS	1001:1002	MS	1001:1002	The HPLC-mass spectrometry (MS)/MS results showed that the IDF may contain methyl-8α-hydroxy-grindelate-7β-O-7'β-ether hydrate, hydroxydecanoic acid, and malyngic acid.
33078401	5	67	theme	HPLC-mass	977:985	arg1	spectrometry					987:998	The HPLC-mass spectrometry	973:998	The HPLC-mass spectrometry (MS)/MS results	973:1014	The HPLC-mass spectrometry (MS)/MS results showed that the IDF may contain methyl-8α-hydroxy-grindelate-7β-O-7'β-ether hydrate, hydroxydecanoic acid, and malyngic acid.
33078401	6	68	from	algae	1200:1204	arg1	Polysaccharides					1165:1179	Polysaccharides	1165:1179	Polysaccharides of high content in algae	1165:1204	PRACTICAL APPLICATION: Polysaccharides of high content in algae cannot be digested by humans, hence regarded as dietary fibers.
33078401	3	69	theme	infrared	564:571	arg1	spectra					584:590	the infrared and UV-Vis spectra	560:590	the infrared and UV-Vis spectra that the SDF and IDF of algae may contain cellulose	560:642	It can be inferred from the infrared and UV-Vis spectra that the SDF and IDF of algae may contain cellulose, hemicellulose, various monosaccharides, phenolic compounds, and quinone pigments.
33078401	0	70	theme	property	42:49	arg1	analysis					12:19	Comparative analysis	0:19	Comparative analysis of the morphological property and chemical composition of soluble and insoluble dietary fiber with bound phenolic compounds from different algae.	0:165	Comparative analysis of the morphological property and chemical composition of soluble and insoluble dietary fiber with bound phenolic compounds from different algae.
33078401	8	71	theme	seaweed-based	1436:1448	arg1	foods					1461:1465	seaweed-based functional foods	1436:1465	seaweed-based functional foods	1436:1465	These topics are important to the development of seaweed-based functional foods.
33078401	1	72	theme	insoluble	245:253	arg1	fiber					263:267	soluble and insoluble dietary fiber	233:267	soluble and insoluble dietary fiber	233:267	The morphological, physicochemical, and biochemical properties of soluble and insoluble dietary fiber from seven types of algae were investigated.
33078401	2	73	theme	fiber	477:481	arg1	contents					489:496	the insoluble dietary fiber (IDF) contents	455:496	the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber)	455:533	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae were significantly lower than the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber).
33078401	0	74	theme	chemical	55:62	arg1	composition					64:74	chemical composition	55:74	chemical composition	55:74	Comparative analysis of the morphological property and chemical composition of soluble and insoluble dietary fiber with bound phenolic compounds from different algae.
33078401	4	75	from	activities	875:884	arg1	IDF					893:895	the IDF	889:895	the IDF	889:895	The bound phenolic in the seven algae varied widely in contents (3.76 to 14.08 mg GAE/g in IDF and 1.94 to 8.61 mg GAE/g in SDF), whose antioxidant activities in the IDF were stronger than those in SDF because of different phenolic compositions.
33078401	0	76	dep	property	42:49	arg1	the					24:26	the	24:26	the	24:26	Comparative analysis of the morphological property and chemical composition of soluble and insoluble dietary fiber with bound phenolic compounds from different algae.
33078401	8	77	theme	foods	1461:1465	arg1	development					1421:1431	the development	1417:1431	the development of seaweed-based functional foods	1417:1465	These topics are important to the development of seaweed-based functional foods.
33078401	0	78	with	analysis	12:19	arg1	compounds					135:143	bound phenolic compounds	120:143	bound phenolic compounds from different algae	120:164	Comparative analysis of the morphological property and chemical composition of soluble and insoluble dietary fiber with bound phenolic compounds from different algae.
33078401	2	79	theme	SDF	341:343	arg1	contents					346:353	The soluble dietary fiber (SDF) contents	314:353	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae	314:423	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae were significantly lower than the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber).
33078401	2	79	theme	SDF	341:343	arg1	lower					444:448	lower	444:448	lower	444:448	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae were significantly lower than the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber).
33078401	6	80	from	content	1189:1195	arg1	algae					1200:1204	algae	1200:1204	algae	1200:1204	PRACTICAL APPLICATION: Polysaccharides of high content in algae cannot be digested by humans, hence regarded as dietary fibers.
33078401	7	81	theme	fibers	1379:1384	arg1	activities					1357:1366	the biological activities	1342:1366	the biological activities of dietary fibers	1342:1384	A large amount of bound phenolic compounds in dietary fibers can add to the biological activities of dietary fibers.
33078401	3	82	theme	UV-Vis	577:582	arg1	spectra					584:590	the infrared and UV-Vis spectra	560:590	the infrared and UV-Vis spectra that the SDF and IDF of algae may contain cellulose	560:642	It can be inferred from the infrared and UV-Vis spectra that the SDF and IDF of algae may contain cellulose, hemicellulose, various monosaccharides, phenolic compounds, and quinone pigments.
33078401	2	83	dep	60.90	364:368	arg1	to					361:362	to	361:362	to	361:362	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae were significantly lower than the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber).
33078401	2	84	theme	fiber	334:338	arg1	contents					346:353	The soluble dietary fiber (SDF) contents	314:353	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae	314:423	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae were significantly lower than the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber).
33078401	2	84	theme	fiber	334:338	arg1	lower					444:448	lower	444:448	lower	444:448	The soluble dietary fiber (SDF) contents (6.48 to 60.90% of the total fiber) in most of the investigated algae were significantly lower than the insoluble dietary fiber (IDF) contents (39.10 to 93.52% of the total fiber).
33078401	5	85	theme	hydroxydecanoic	1101:1115	arg1	acid					1117:1120	hydroxydecanoic acid	1101:1120	hydroxydecanoic acid	1101:1120	The HPLC-mass spectrometry (MS)/MS results showed that the IDF may contain methyl-8α-hydroxy-grindelate-7β-O-7'β-ether hydrate, hydroxydecanoic acid, and malyngic acid.
33078401	4	86	theme	different	940:948	arg1	compositions					959:970	different phenolic compositions	940:970	different phenolic compositions	940:970	The bound phenolic in the seven algae varied widely in contents (3.76 to 14.08 mg GAE/g in IDF and 1.94 to 8.61 mg GAE/g in SDF), whose antioxidant activities in the IDF were stronger than those in SDF because of different phenolic compositions.
33078401	1	87	theme	dietary	255:261	arg1	fiber					263:267	soluble and insoluble dietary fiber	233:267	soluble and insoluble dietary fiber	233:267	The morphological, physicochemical, and biochemical properties of soluble and insoluble dietary fiber from seven types of algae were investigated.
35153754	4	0	theme	WPEI	523:526	arg1	minerals					484:491	minerals	484:491	minerals	484:491	Proximate compositions, minerals, and amino acids of the DPEI, WPEI, and APEI were determined.
35153754	4	0	theme	WPEI	523:526	arg1	compositions					470:481	Proximate compositions	460:481	Proximate compositions	460:481	Proximate compositions, minerals, and amino acids of the DPEI, WPEI, and APEI were determined.
35153754	4	0	theme	WPEI	523:526	arg1	acids					504:508	amino acids	498:508	amino acids	498:508	Proximate compositions, minerals, and amino acids of the DPEI, WPEI, and APEI were determined.
35153754	5	1	theme	polysaccharides	590:604	arg1	effect					576:581	The growth-promoting effect	555:581	The growth-promoting effect of the polysaccharides on selected intestinal microflora	555:638	The growth-promoting effect of the polysaccharides on selected intestinal microflora was determined based on the plate count method.
35153754	14	2	theme	carbon	1537:1542	arg1	propulsion					1544:1553	the intestinal activated carbon propulsion	1512:1553	the intestinal activated carbon propulsion of the WPEI, APEI, and DPEI fed rats	1512:1590	Also, the intestinal activated carbon propulsion of the WPEI, APEI, and DPEI fed rats increased.
35153754	12	3	theme	other	1303:1307	arg1	groups					1309:1314	the other groups	1299:1314	the other groups	1299:1314	The average final body weight of the experimental rats treated with DPEI was also lower than the other groups.
35153754	12	4	theme	rats	1256:1259	arg1	lower					1288:1292	lower	1288:1292	lower	1288:1292	The average final body weight of the experimental rats treated with DPEI was also lower than the other groups.
35153754	12	4	theme	rats	1256:1259	arg1	weight					1229:1234	The average final body weight	1206:1234	The average final body weight of the experimental rats treated with DPEI	1206:1277	The average final body weight of the experimental rats treated with DPEI was also lower than the other groups.
35153754	15	5	theme	fatty	1625:1629	arg1	content					1637:1643	content	1637:1643	content	1637:1643	Among the short-chain fatty acids content determined in the fecal samples, the propionic acid content of the WPEI group was significantly highest.
35153754	15	5	theme	fatty	1625:1629	arg1	acids					1631:1635	the short-chain fatty acids	1609:1635	the short-chain fatty acids content determined in the fecal samples	1609:1675	Among the short-chain fatty acids content determined in the fecal samples, the propionic acid content of the WPEI group was significantly highest.
35153754	5	6	theme	selected	609:616	arg1	microflora					629:638	selected intestinal microflora	609:638	selected intestinal microflora	609:638	The growth-promoting effect of the polysaccharides on selected intestinal microflora was determined based on the plate count method.
35153754	14	7	theme	intestinal	1516:1525	arg1	propulsion					1544:1553	the intestinal activated carbon propulsion	1512:1553	the intestinal activated carbon propulsion of the WPEI, APEI, and DPEI fed rats	1512:1590	Also, the intestinal activated carbon propulsion of the WPEI, APEI, and DPEI fed rats increased.
35153754	5	8	theme	growth-promoting	559:574	arg1	effect					576:581	The growth-promoting effect	555:581	The growth-promoting effect of the polysaccharides on selected intestinal microflora	555:638	The growth-promoting effect of the polysaccharides on selected intestinal microflora was determined based on the plate count method.
35153754	6	9	theme	DPEI	723:726	arg1	effect					713:718	the in vivo effect	701:718	the in vivo effect of DPEI and its polysaccharides on the intestinal function of the SD rats	701:792	In contrast, the in vivo effect of DPEI and its polysaccharides on the intestinal function of the SD rats was determined.
35153754	1	10	theme	Enteromorpha	178:189	arg1	intestinalis					191:202	Enteromorpha intestinalis	178:202	Enteromorpha intestinalis (EI)	178:207	This study aims to determine the effect of polysaccharides extracted from Enteromorpha intestinalis (EI) on the intestinal function of Sprague Dawley (SD) rats.
35153754	1	10	theme	Enteromorpha	178:189	arg1	EI					205:206	EI	205:206	EI	205:206	This study aims to determine the effect of polysaccharides extracted from Enteromorpha intestinalis (EI) on the intestinal function of Sprague Dawley (SD) rats.
35153754	1	11	theme	rats	259:262	arg1	function					227:234	the intestinal function	212:234	the intestinal function of Sprague Dawley (SD) rats	212:262	This study aims to determine the effect of polysaccharides extracted from Enteromorpha intestinalis (EI) on the intestinal function of Sprague Dawley (SD) rats.
35153754	8	12	theme	lower	892:896	arg1	sugars					904:909	lower total sugars	892:909	lower total sugars	892:909	The result showed that APEI had lower total sugars and total proteins content than the WPEI.
35153754	15	13	theme	propionic	1682:1690	arg1	highest					1741:1747	highest	1741:1747	highest	1741:1747	Among the short-chain fatty acids content determined in the fecal samples, the propionic acid content of the WPEI group was significantly highest.
35153754	15	13	theme	propionic	1682:1690	arg1	content					1697:1703	the propionic acid content	1678:1703	the propionic acid content of the WPEI group	1678:1721	Among the short-chain fatty acids content determined in the fecal samples, the propionic acid content of the WPEI group was significantly highest.
35153754	5	14	theme	plate	668:672	arg1	method					680:685	the plate count method	664:685	the plate count method	664:685	The growth-promoting effect of the polysaccharides on selected intestinal microflora was determined based on the plate count method.
35153754	0	15	from	Function	71:78	arg1	Rats					98:101	Sprague Dawley Rats	83:101	Sprague Dawley Rats	83:101	Effect of Polysaccharides From Enteromorpha intestinalis on Intestinal Function in Sprague Dawley Rats.
35153754	12	16	theme	final	1218:1222	arg1	lower					1288:1292	lower	1288:1292	lower	1288:1292	The average final body weight of the experimental rats treated with DPEI was also lower than the other groups.
35153754	12	16	theme	final	1218:1222	arg1	weight					1229:1234	The average final body weight	1206:1234	The average final body weight of the experimental rats treated with DPEI	1206:1277	The average final body weight of the experimental rats treated with DPEI was also lower than the other groups.
35153754	15	17	theme	fecal	1663:1667	arg1	samples					1669:1675	the fecal samples	1659:1675	the fecal samples	1659:1675	Among the short-chain fatty acids content determined in the fecal samples, the propionic acid content of the WPEI group was significantly highest.
35153754	16	18	theme	intestinal	1799:1808	arg1	digestion					1810:1818	intestinal digestion	1799:1818	intestinal digestion	1799:1818	Therefore, WPEI had the best effect in improving intestinal digestion.
35153754	13	19	theme	control	1382:1388	arg1	rats					1390:1393	the control rats	1378:1393	the control rats	1378:1393	The pH value of the feces of all treated rats was lower than the control rats, and the moisture content of the fecal samples of these experimental groups was higher than the control group.
35153754	13	20	theme	treated	1350:1356	arg1	rats					1358:1361	all treated rats	1346:1361	all treated rats	1346:1361	The pH value of the feces of all treated rats was lower than the control rats, and the moisture content of the fecal samples of these experimental groups was higher than the control group.
35153754	14	21	theme	activated	1527:1535	arg1	propulsion					1544:1553	the intestinal activated carbon propulsion	1512:1553	the intestinal activated carbon propulsion of the WPEI, APEI, and DPEI fed rats	1512:1590	Also, the intestinal activated carbon propulsion of the WPEI, APEI, and DPEI fed rats increased.
35153754	0	22	from	Effect	0:5	arg1	intestinalis					44:55	Enteromorpha intestinalis	31:55	Enteromorpha intestinalis	31:55	Effect of Polysaccharides From Enteromorpha intestinalis on Intestinal Function in Sprague Dawley Rats.
35153754	0	22	from	Effect	0:5	arg1	Function					71:78	Intestinal Function	60:78	Intestinal Function in Sprague Dawley Rats	60:101	Effect of Polysaccharides From Enteromorpha intestinalis on Intestinal Function in Sprague Dawley Rats.
35153754	11	23	theme	intestinal	1116:1125	arg1	peristalsis					1127:1137	intestinal peristalsis	1116:1137	intestinal peristalsis	1116:1137	The in vivo study showed that WPEI improved intestinal peristalsis and other intestinal functions compared with the other rat groups.
35153754	8	24	theme	total	915:919	arg1	content					930:936	total proteins content	915:936	total proteins content	915:936	The result showed that APEI had lower total sugars and total proteins content than the WPEI.
35153754	1	25	from	effect	137:142	arg1	function					227:234	the intestinal function	212:234	the intestinal function of Sprague Dawley (SD) rats	212:262	This study aims to determine the effect of polysaccharides extracted from Enteromorpha intestinalis (EI) on the intestinal function of Sprague Dawley (SD) rats.
35153754	15	26	theme	WPEI	1712:1715	arg1	group					1717:1721	the WPEI group	1708:1721	the WPEI group	1708:1721	Among the short-chain fatty acids content determined in the fecal samples, the propionic acid content of the WPEI group was significantly highest.
35153754	11	27	theme	intestinal	1149:1158	arg1	functions					1160:1168	other intestinal functions	1143:1168	other intestinal functions	1143:1168	The in vivo study showed that WPEI improved intestinal peristalsis and other intestinal functions compared with the other rat groups.
35153754	13	28	theme	feces	1337:1341	arg1	value					1324:1328	The pH value	1317:1328	The pH value of the feces of all treated rats	1317:1361	The pH value of the feces of all treated rats was lower than the control rats, and the moisture content of the fecal samples of these experimental groups was higher than the control group.
35153754	13	28	theme	feces	1337:1341	arg1	lower					1367:1371	lower	1367:1371	lower	1367:1371	The pH value of the feces of all treated rats was lower than the control rats, and the moisture content of the fecal samples of these experimental groups was higher than the control group.
35153754	5	29	from	effect	576:581	arg1	microflora					629:638	selected intestinal microflora	609:638	selected intestinal microflora	609:638	The growth-promoting effect of the polysaccharides on selected intestinal microflora was determined based on the plate count method.
35153754	7	30	theme	%	836:836	arg1	WPEI					838:841	1% WPEI	835:841	1% WPEI	835:841	These rats were fed with 1% WPEI, APEI, and DPEI.
35153754	13	31	theme	experimental	1451:1462	arg1	groups					1464:1469	these experimental groups	1445:1469	these experimental groups	1445:1469	The pH value of the feces of all treated rats was lower than the control rats, and the moisture content of the fecal samples of these experimental groups was higher than the control group.
35153754	0	32	theme	Polysaccharides	10:24	arg1	Effect					0:5	Effect	0:5	Effect of Polysaccharides From Enteromorpha intestinalis on Intestinal Function in Sprague Dawley Rats.	0:102	Effect of Polysaccharides From Enteromorpha intestinalis on Intestinal Function in Sprague Dawley Rats.
35153754	13	33	theme	pH	1321:1322	arg1	value					1324:1328	The pH value	1317:1328	The pH value of the feces of all treated rats	1317:1361	The pH value of the feces of all treated rats was lower than the control rats, and the moisture content of the fecal samples of these experimental groups was higher than the control group.
35153754	13	33	theme	pH	1321:1322	arg1	lower					1367:1371	lower	1367:1371	lower	1367:1371	The pH value of the feces of all treated rats was lower than the control rats, and the moisture content of the fecal samples of these experimental groups was higher than the control group.
35153754	3	34	theme	dried	420:424	arg1	powder					426:431	The dried powder	416:431	The dried powder of EI	416:437	The dried powder of EI was labeled as DPEI.
35153754	3	34	theme	dried	420:424	arg1	DPEI					454:457	DPEI	454:457	DPEI	454:457	The dried powder of EI was labeled as DPEI.
35153754	0	35	theme	Enteromorpha	31:42	arg1	intestinalis					44:55	Enteromorpha intestinalis	31:55	Enteromorpha intestinalis	31:55	Effect of Polysaccharides From Enteromorpha intestinalis on Intestinal Function in Sprague Dawley Rats.
35153754	14	36	theme	DPEI	1578:1581	arg1	propulsion					1544:1553	the intestinal activated carbon propulsion	1512:1553	the intestinal activated carbon propulsion of the WPEI, APEI, and DPEI fed rats	1512:1590	Also, the intestinal activated carbon propulsion of the WPEI, APEI, and DPEI fed rats increased.
35153754	6	37	theme	SD	786:787	arg1	rats					789:792	the SD rats	782:792	the SD rats	782:792	In contrast, the in vivo effect of DPEI and its polysaccharides on the intestinal function of the SD rats was determined.
35153754	16	38	contain	had	1766:1768	arg1	WPEI					1761:1764	WPEI	1761:1764	WPEI	1761:1764	Therefore, WPEI had the best effect in improving intestinal digestion.
35153754	16	38	contain	had	1766:1768	arg2	effect					1779:1784	the best effect	1770:1784	the best effect	1770:1784	Therefore, WPEI had the best effect in improving intestinal digestion.
35153754	13	39	theme	fecal	1428:1432	arg1	samples					1434:1440	the fecal samples	1424:1440	the fecal samples of these experimental groups	1424:1469	The pH value of the feces of all treated rats was lower than the control rats, and the moisture content of the fecal samples of these experimental groups was higher than the control group.
35153754	7	40	theme	1	835:835	arg1	%					836:836	%	836:836	%	836:836	These rats were fed with 1% WPEI, APEI, and DPEI.
35153754	1	41	theme	intestinal	216:225	arg1	function					227:234	the intestinal function	212:234	the intestinal function of Sprague Dawley (SD) rats	212:262	This study aims to determine the effect of polysaccharides extracted from Enteromorpha intestinalis (EI) on the intestinal function of Sprague Dawley (SD) rats.
35153754	6	42	theme	intestinal	759:768	arg1	function					770:777	the intestinal function	755:777	the intestinal function of the SD rats	755:792	In contrast, the in vivo effect of DPEI and its polysaccharides on the intestinal function of the SD rats was determined.
35153754	4	43	theme	APEI	533:536	arg1	minerals					484:491	minerals	484:491	minerals	484:491	Proximate compositions, minerals, and amino acids of the DPEI, WPEI, and APEI were determined.
35153754	4	43	theme	APEI	533:536	arg1	compositions					470:481	Proximate compositions	460:481	Proximate compositions	460:481	Proximate compositions, minerals, and amino acids of the DPEI, WPEI, and APEI were determined.
35153754	4	43	theme	APEI	533:536	arg1	acids					504:508	amino acids	498:508	amino acids	498:508	Proximate compositions, minerals, and amino acids of the DPEI, WPEI, and APEI were determined.
35153754	13	44	theme	control	1491:1497	arg1	group					1499:1503	the control group	1487:1503	the control group	1487:1503	The pH value of the feces of all treated rats was lower than the control rats, and the moisture content of the fecal samples of these experimental groups was higher than the control group.
35153754	15	45	theme	acid	1692:1695	arg1	highest					1741:1747	highest	1741:1747	highest	1741:1747	Among the short-chain fatty acids content determined in the fecal samples, the propionic acid content of the WPEI group was significantly highest.
35153754	15	45	theme	acid	1692:1695	arg1	content					1697:1703	the propionic acid content	1678:1703	the propionic acid content of the WPEI group	1678:1721	Among the short-chain fatty acids content determined in the fecal samples, the propionic acid content of the WPEI group was significantly highest.
35153754	2	46	theme	alkaline	336:343	arg1	solution					345:352	alkaline solution	336:352	alkaline solution	336:352	The polysaccharides were extracted from the green alga using water and alkaline solution, where these extracts were named WPEI and APEI, respectively.
35153754	1	47	theme	Dawley	247:252	arg1	rats					259:262	Sprague Dawley (SD) rats	239:262	Sprague Dawley (SD) rats	239:262	This study aims to determine the effect of polysaccharides extracted from Enteromorpha intestinalis (EI) on the intestinal function of Sprague Dawley (SD) rats.
35153754	6	48	dep	in	705:706	arg1	vivo					708:711	vivo	708:711	vivo	708:711	In contrast, the in vivo effect of DPEI and its polysaccharides on the intestinal function of the SD rats was determined.
35153754	15	49	theme	short-chain	1613:1623	arg1	content					1637:1643	content	1637:1643	content	1637:1643	Among the short-chain fatty acids content determined in the fecal samples, the propionic acid content of the WPEI group was significantly highest.
35153754	15	49	theme	short-chain	1613:1623	arg1	acids					1631:1635	the short-chain fatty acids	1609:1635	the short-chain fatty acids content determined in the fecal samples	1609:1675	Among the short-chain fatty acids content determined in the fecal samples, the propionic acid content of the WPEI group was significantly highest.
35153754	6	50	theme	polysaccharides	736:750	arg1	effect					713:718	the in vivo effect	701:718	the in vivo effect of DPEI and its polysaccharides on the intestinal function of the SD rats	701:792	In contrast, the in vivo effect of DPEI and its polysaccharides on the intestinal function of the SD rats was determined.
35153754	13	51	theme	moisture	1404:1411	arg1	content					1413:1419	the moisture content	1400:1419	the moisture content of the fecal samples of these experimental groups	1400:1469	The pH value of the feces of all treated rats was lower than the control rats, and the moisture content of the fecal samples of these experimental groups was higher than the control group.
35153754	13	51	theme	moisture	1404:1411	arg1	higher					1475:1480	higher	1475:1480	higher	1475:1480	The pH value of the feces of all treated rats was lower than the control rats, and the moisture content of the fecal samples of these experimental groups was higher than the control group.
35153754	1	52	theme	SD	255:256	arg1	rats					259:262	Sprague Dawley (SD) rats	239:262	Sprague Dawley (SD) rats	239:262	This study aims to determine the effect of polysaccharides extracted from Enteromorpha intestinalis (EI) on the intestinal function of Sprague Dawley (SD) rats.
35153754	12	53	theme	experimental	1243:1254	arg1	rats					1256:1259	the experimental rats	1239:1259	the experimental rats treated with DPEI	1239:1277	The average final body weight of the experimental rats treated with DPEI was also lower than the other groups.
35153754	11	54	theme	rat	1194:1196	arg1	groups					1198:1203	the other rat groups	1184:1203	the other rat groups	1184:1203	The in vivo study showed that WPEI improved intestinal peristalsis and other intestinal functions compared with the other rat groups.
35153754	8	55	theme	total	898:902	arg1	sugars					904:909	lower total sugars	892:909	lower total sugars	892:909	The result showed that APEI had lower total sugars and total proteins content than the WPEI.
35153754	12	56	theme	body	1224:1227	arg1	lower					1288:1292	lower	1288:1292	lower	1288:1292	The average final body weight of the experimental rats treated with DPEI was also lower than the other groups.
35153754	12	56	theme	body	1224:1227	arg1	weight					1229:1234	The average final body weight	1206:1234	The average final body weight of the experimental rats treated with DPEI	1206:1277	The average final body weight of the experimental rats treated with DPEI was also lower than the other groups.
35153754	15	57	dep	determined	1645:1654	arg1	content					1637:1643	content	1637:1643	content	1637:1643	Among the short-chain fatty acids content determined in the fecal samples, the propionic acid content of the WPEI group was significantly highest.
35153754	15	57	dep	determined	1645:1654	arg1	acids					1631:1635	the short-chain fatty acids	1609:1635	the short-chain fatty acids content determined in the fecal samples	1609:1675	Among the short-chain fatty acids content determined in the fecal samples, the propionic acid content of the WPEI group was significantly highest.
35153754	5	58	theme	intestinal	618:627	arg1	microflora					629:638	selected intestinal microflora	609:638	selected intestinal microflora	609:638	The growth-promoting effect of the polysaccharides on selected intestinal microflora was determined based on the plate count method.
35153754	12	59	theme	average	1210:1216	arg1	lower					1288:1292	lower	1288:1292	lower	1288:1292	The average final body weight of the experimental rats treated with DPEI was also lower than the other groups.
35153754	12	59	theme	average	1210:1216	arg1	weight					1229:1234	The average final body weight	1206:1234	The average final body weight of the experimental rats treated with DPEI	1206:1277	The average final body weight of the experimental rats treated with DPEI was also lower than the other groups.
35153754	13	60	theme	rats	1358:1361	arg1	feces					1337:1341	the feces	1333:1341	the feces of all treated rats	1333:1361	The pH value of the feces of all treated rats was lower than the control rats, and the moisture content of the fecal samples of these experimental groups was higher than the control group.
35153754	11	61	dep	in	1076:1077	arg1	vivo					1079:1082	vivo	1079:1082	vivo	1079:1082	The in vivo study showed that WPEI improved intestinal peristalsis and other intestinal functions compared with the other rat groups.
35153754	4	62	theme	Proximate	460:468	arg1	compositions					470:481	Proximate compositions	460:481	Proximate compositions	460:481	Proximate compositions, minerals, and amino acids of the DPEI, WPEI, and APEI were determined.
35153754	11	63	theme	other	1188:1192	arg1	groups					1198:1203	the other rat groups	1184:1203	the other rat groups	1184:1203	The in vivo study showed that WPEI improved intestinal peristalsis and other intestinal functions compared with the other rat groups.
35153754	8	64	contain	had	888:890	arg2	sugars					904:909	lower total sugars	892:909	lower total sugars	892:909	The result showed that APEI had lower total sugars and total proteins content than the WPEI.
35153754	8	64	contain	had	888:890	arg2	content					930:936	total proteins content	915:936	total proteins content	915:936	The result showed that APEI had lower total sugars and total proteins content than the WPEI.
35153754	8	64	contain	had	888:890	arg1	APEI					883:886	APEI	883:886	APEI	883:886	The result showed that APEI had lower total sugars and total proteins content than the WPEI.
35153754	0	65	theme	Dawley	91:96	arg1	Rats					98:101	Sprague Dawley Rats	83:101	Sprague Dawley Rats	83:101	Effect of Polysaccharides From Enteromorpha intestinalis on Intestinal Function in Sprague Dawley Rats.
35153754	1	66	theme	polysaccharides	147:161	arg1	effect					137:142	the effect	133:142	the effect of polysaccharides extracted from Enteromorpha intestinalis (EI) on the intestinal function of Sprague Dawley (SD) rats	133:262	This study aims to determine the effect of polysaccharides extracted from Enteromorpha intestinalis (EI) on the intestinal function of Sprague Dawley (SD) rats.
35153754	11	67	theme	other	1143:1147	arg1	functions					1160:1168	other intestinal functions	1143:1168	other intestinal functions	1143:1168	The in vivo study showed that WPEI improved intestinal peristalsis and other intestinal functions compared with the other rat groups.
35153754	5	68	theme	count	674:678	arg1	method					680:685	the plate count method	664:685	the plate count method	664:685	The growth-promoting effect of the polysaccharides on selected intestinal microflora was determined based on the plate count method.
35153754	8	69	theme	proteins	921:928	arg1	content					930:936	total proteins content	915:936	total proteins content	915:936	The result showed that APEI had lower total sugars and total proteins content than the WPEI.
35153754	14	70	dep	WPEI	1562:1565	arg1	rats					1587:1590	fed rats	1583:1590	fed rats	1583:1590	Also, the intestinal activated carbon propulsion of the WPEI, APEI, and DPEI fed rats increased.
35153754	14	70	dep	WPEI	1562:1565	arg1	the					1558:1560	the	1558:1560	the	1558:1560	Also, the intestinal activated carbon propulsion of the WPEI, APEI, and DPEI fed rats increased.
35153754	0	71	from	intestinalis	44:55	arg1	Polysaccharides					10:24	Polysaccharides	10:24	Polysaccharides From Enteromorpha intestinalis	10:55	Effect of Polysaccharides From Enteromorpha intestinalis on Intestinal Function in Sprague Dawley Rats.
35153754	0	71	from	intestinalis	44:55	arg1	Effect					0:5	Effect	0:5	Effect of Polysaccharides From Enteromorpha intestinalis on Intestinal Function in Sprague Dawley Rats.	0:102	Effect of Polysaccharides From Enteromorpha intestinalis on Intestinal Function in Sprague Dawley Rats.
35153754	15	72	theme	group	1717:1721	arg1	highest					1741:1747	highest	1741:1747	highest	1741:1747	Among the short-chain fatty acids content determined in the fecal samples, the propionic acid content of the WPEI group was significantly highest.
35153754	15	72	theme	group	1717:1721	arg1	content					1697:1703	the propionic acid content	1678:1703	the propionic acid content of the WPEI group	1678:1721	Among the short-chain fatty acids content determined in the fecal samples, the propionic acid content of the WPEI group was significantly highest.
35153754	14	73	theme	WPEI	1562:1565	arg1	propulsion					1544:1553	the intestinal activated carbon propulsion	1512:1553	the intestinal activated carbon propulsion of the WPEI, APEI, and DPEI fed rats	1512:1590	Also, the intestinal activated carbon propulsion of the WPEI, APEI, and DPEI fed rats increased.
35153754	9	74	contain	contain	966:972	arg2	arabinose					974:982	arabinose	974:982	arabinose	974:982	WPEI did not contain arabinose.
35153754	9	74	contain	contain	966:972	arg1	WPEI					953:956	WPEI	953:956	WPEI	953:956	WPEI did not contain arabinose.
35153754	16	75	theme	best	1774:1777	arg1	effect					1779:1784	the best effect	1770:1784	the best effect	1770:1784	Therefore, WPEI had the best effect in improving intestinal digestion.
35153754	6	76	from	effect	713:718	arg1	function					770:777	the intestinal function	755:777	the intestinal function of the SD rats	755:792	In contrast, the in vivo effect of DPEI and its polysaccharides on the intestinal function of the SD rats was determined.
35153754	4	77	theme	DPEI	517:520	arg1	minerals					484:491	minerals	484:491	minerals	484:491	Proximate compositions, minerals, and amino acids of the DPEI, WPEI, and APEI were determined.
35153754	4	77	theme	DPEI	517:520	arg1	compositions					470:481	Proximate compositions	460:481	Proximate compositions	460:481	Proximate compositions, minerals, and amino acids of the DPEI, WPEI, and APEI were determined.
35153754	4	77	theme	DPEI	517:520	arg1	acids					504:508	amino acids	498:508	amino acids	498:508	Proximate compositions, minerals, and amino acids of the DPEI, WPEI, and APEI were determined.
35153754	13	78	theme	groups	1464:1469	arg1	samples					1434:1440	the fecal samples	1424:1440	the fecal samples of these experimental groups	1424:1469	The pH value of the feces of all treated rats was lower than the control rats, and the moisture content of the fecal samples of these experimental groups was higher than the control group.
35153754	3	79	theme	EI	436:437	arg1	powder					426:431	The dried powder	416:431	The dried powder of EI	416:437	The dried powder of EI was labeled as DPEI.
35153754	3	79	theme	EI	436:437	arg1	DPEI					454:457	DPEI	454:457	DPEI	454:457	The dried powder of EI was labeled as DPEI.
35153754	14	80	theme	fed	1583:1585	arg1	rats					1587:1590	fed rats	1583:1590	fed rats	1583:1590	Also, the intestinal activated carbon propulsion of the WPEI, APEI, and DPEI fed rats increased.
35153754	6	81	theme	rats	789:792	arg1	function					770:777	the intestinal function	755:777	the intestinal function of the SD rats	755:792	In contrast, the in vivo effect of DPEI and its polysaccharides on the intestinal function of the SD rats was determined.
35153754	11	82	theme	in	1076:1077	arg1	study					1084:1088	The in vivo study	1072:1088	The in vivo study	1072:1088	The in vivo study showed that WPEI improved intestinal peristalsis and other intestinal functions compared with the other rat groups.
35153754	6	83	theme	in	705:706	arg1	effect					713:718	the in vivo effect	701:718	the in vivo effect of DPEI and its polysaccharides on the intestinal function of the SD rats	701:792	In contrast, the in vivo effect of DPEI and its polysaccharides on the intestinal function of the SD rats was determined.
35153754	0	84	theme	Intestinal	60:69	arg1	Function					71:78	Intestinal Function	60:78	Intestinal Function in Sprague Dawley Rats	60:101	Effect of Polysaccharides From Enteromorpha intestinalis on Intestinal Function in Sprague Dawley Rats.
35153754	10	85	theme	better	1014:1019	arg1	ability					1021:1027	a better ability	1012:1027	a better ability to promote microbial growth than the DPEI	1012:1069	The WPEI and APEI also had a better ability to promote microbial growth than the DPEI.
35153754	4	86	theme	amino	498:502	arg1	acids					504:508	amino acids	498:508	amino acids	498:508	Proximate compositions, minerals, and amino acids of the DPEI, WPEI, and APEI were determined.
35153754	13	87	theme	samples	1434:1440	arg1	content					1413:1419	the moisture content	1400:1419	the moisture content of the fecal samples of these experimental groups	1400:1469	The pH value of the feces of all treated rats was lower than the control rats, and the moisture content of the fecal samples of these experimental groups was higher than the control group.
35153754	13	87	theme	samples	1434:1440	arg1	higher					1475:1480	higher	1475:1480	higher	1475:1480	The pH value of the feces of all treated rats was lower than the control rats, and the moisture content of the fecal samples of these experimental groups was higher than the control group.
35153754	14	88	theme	APEI	1568:1571	arg1	propulsion					1544:1553	the intestinal activated carbon propulsion	1512:1553	the intestinal activated carbon propulsion of the WPEI, APEI, and DPEI fed rats	1512:1590	Also, the intestinal activated carbon propulsion of the WPEI, APEI, and DPEI fed rats increased.
35153754	10	89	contain	had	1008:1010	arg2	ability					1021:1027	a better ability	1012:1027	a better ability to promote microbial growth than the DPEI	1012:1069	The WPEI and APEI also had a better ability to promote microbial growth than the DPEI.
35153754	10	89	contain	had	1008:1010	arg1	APEI					998:1001	APEI	998:1001	APEI	998:1001	The WPEI and APEI also had a better ability to promote microbial growth than the DPEI.
35153754	10	89	contain	had	1008:1010	arg1	WPEI					989:992	WPEI	989:992	WPEI	989:992	The WPEI and APEI also had a better ability to promote microbial growth than the DPEI.
35153754	2	90	theme	green	309:313	arg1	alga					315:318	the green alga	305:318	the green alga	305:318	The polysaccharides were extracted from the green alga using water and alkaline solution, where these extracts were named WPEI and APEI, respectively.
35153754	10	91	theme	microbial	1040:1048	arg1	growth					1050:1055	microbial growth	1040:1055	microbial growth	1040:1055	The WPEI and APEI also had a better ability to promote microbial growth than the DPEI.
32421690	2	0	theme	low	562:564	arg1	efficiency					576:585	low digestive efficiency	562:585	low digestive efficiency	562:585	By selecting chicken lines divergently for their digestive efficiency, we showed previously that digestive efficiency is under genetic control and that the two resulting divergent lines, D+ (high digestive efficiency or "digestibility +") and D- (low digestive efficiency or "digestibility -"), also differ for the abundance of specific bacteria in their caeca.
32421690	8	1	theme	caecal	1723:1728	arg1	composition					1751:1761	the caecal microbiota taxonomic composition	1719:1761	the caecal microbiota taxonomic composition	1719:1761	These results confirm the impact of the genetic selection led on digestibility on the caecal microbiota taxonomic composition.
32421690	6	2	theme	digestive	1322:1330	arg1	efficiency					1332:1341	digestive efficiency	1322:1341	digestive efficiency	1322:1341	The caecal microbiota was the most impacted by the differences in digestive efficiency, with 41 bacterial species with abundances differing between highly and poorly efficient birds.
32421690	1	3	theme	human	246:250	arg1	consumption					252:262	human consumption	246:262	human consumption	246:262	Improving the digestive efficiency of broiler chickens (Gallus gallus) could reduce organic waste, increase the use of alternative feed not used for human consumption and reduce the impact of feed in production costs.
32421690	3	4	theme	x	922:922	arg1	progeny					931:937	a [D+ x D-] F8 progeny	916:937	a [D+ x D-] F8 progeny of 200 individuals	916:956	Here we perform a more extensive census of the bacteria present in the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals.
32421690	8	5	theme	taxonomic	1741:1749	arg1	composition					1751:1761	the caecal microbiota taxonomic composition	1719:1761	the caecal microbiota taxonomic composition	1719:1761	These results confirm the impact of the genetic selection led on digestibility on the caecal microbiota taxonomic composition.
32421690	6	6	theme	efficient	1422:1430	arg1	birds					1432:1436	highly and poorly efficient birds	1404:1436	highly and poorly efficient birds	1404:1436	The caecal microbiota was the most impacted by the differences in digestive efficiency, with 41 bacterial species with abundances differing between highly and poorly efficient birds.
32421690	4	7	theme	ileal	998:1002	arg1	microbiotas					1024:1034	the ileal, jejunal and caecal microbiotas	994:1034	the ileal, jejunal and caecal microbiotas	994:1034	We sequenced the 16S rRNA genes of the ileal, jejunal and caecal microbiotas, and compared the compositions and predicted functions of microbiotas from the different intestinal segments for 20 AMEn+ and 19 AMEn- birds.
32421690	2	8	from	abundance	630:638	arg1	caeca					670:674	their caeca	664:674	their caeca	664:674	By selecting chicken lines divergently for their digestive efficiency, we showed previously that digestive efficiency is under genetic control and that the two resulting divergent lines, D+ (high digestive efficiency or "digestibility +") and D- (low digestive efficiency or "digestibility -"), also differ for the abundance of specific bacteria in their caeca.
32421690	1	9	theme	organic	181:187	arg1	waste					189:193	organic waste	181:193	organic waste	181:193	Improving the digestive efficiency of broiler chickens (Gallus gallus) could reduce organic waste, increase the use of alternative feed not used for human consumption and reduce the impact of feed in production costs.
32421690	0	10	theme	microbial	75:83	arg1	ecosystems					85:94	their digestive microbial ecosystems	59:94	their digestive microbial ecosystems	59:94	Broilers divergently selected for digestibility differ for their digestive microbial ecosystems.
32421690	4	11	theme	jejunal	1005:1011	arg1	microbiotas					1024:1034	the ileal, jejunal and caecal microbiotas	994:1034	the ileal, jejunal and caecal microbiotas	994:1034	We sequenced the 16S rRNA genes of the ileal, jejunal and caecal microbiotas, and compared the compositions and predicted functions of microbiotas from the different intestinal segments for 20 AMEn+ and 19 AMEn- birds.
32421690	4	12	theme	rRNA	980:983	arg1	genes					985:989	the 16S rRNA genes	972:989	the 16S rRNA genes of the ileal, jejunal and caecal microbiotas	972:1034	We sequenced the 16S rRNA genes of the ileal, jejunal and caecal microbiotas, and compared the compositions and predicted functions of microbiotas from the different intestinal segments for 20 AMEn+ and 19 AMEn- birds.
32421690	2	13	dep	D-	558:559	arg1	"					606:606	"digestibility -"	590:606	"digestibility -"	590:606	By selecting chicken lines divergently for their digestive efficiency, we showed previously that digestive efficiency is under genetic control and that the two resulting divergent lines, D+ (high digestive efficiency or "digestibility +") and D- (low digestive efficiency or "digestibility -"), also differ for the abundance of specific bacteria in their caeca.
32421690	2	13	dep	D-	558:559	arg1	efficiency					576:585	low digestive efficiency	562:585	low digestive efficiency	562:585	By selecting chicken lines divergently for their digestive efficiency, we showed previously that digestive efficiency is under genetic control and that the two resulting divergent lines, D+ (high digestive efficiency or "digestibility +") and D- (low digestive efficiency or "digestibility -"), also differ for the abundance of specific bacteria in their caeca.
32421690	3	14	theme	extensive	700:708	arg1	census					710:715	a more extensive census	693:715	a more extensive census of the bacteria present in the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals	693:956	Here we perform a more extensive census of the bacteria present in the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals.
32421690	6	15	from	differences	1307:1317	arg1	efficiency					1332:1341	digestive efficiency	1322:1341	digestive efficiency	1322:1341	The caecal microbiota was the most impacted by the differences in digestive efficiency, with 41 bacterial species with abundances differing between highly and poorly efficient birds.
32421690	7	16	theme	efficient	1495:1503	arg1	birds					1505:1509	efficient birds	1495:1509	efficient birds	1495:1509	Furthermore, we predicted that the caecal microbiota of efficient birds might be enriched in genes contributing to the degradation of short chain fatty acids (SCFA) from non-starch polysaccharides.
32421690	1	17	theme	broiler	135:141	arg1	chickens					143:150	broiler chickens	135:150	broiler chickens (Gallus gallus)	135:166	Improving the digestive efficiency of broiler chickens (Gallus gallus) could reduce organic waste, increase the use of alternative feed not used for human consumption and reduce the impact of feed in production costs.
32421690	1	17	theme	broiler	135:141	arg1	gallus					160:165	Gallus gallus	153:165	Gallus gallus	153:165	Improving the digestive efficiency of broiler chickens (Gallus gallus) could reduce organic waste, increase the use of alternative feed not used for human consumption and reduce the impact of feed in production costs.
32421690	3	18	theme	chickens	775:782	arg1	microbiota					758:767	the digestive microbiota	744:767	the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals	744:956	Here we perform a more extensive census of the bacteria present in the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals.
32421690	9	19	theme	host	1841:1844	arg1	genes					1828:1832	specific, causal genes	1811:1832	specific, causal genes of the host controlling variations in the abundances of bacterial taxons	1811:1905	They open the way toward the identification of specific, causal genes of the host controlling variations in the abundances of bacterial taxons.
32421690	4	20	from	functions	1081:1089	arg1	segments					1136:1143	the different intestinal segments	1111:1143	the different intestinal segments	1111:1143	We sequenced the 16S rRNA genes of the ileal, jejunal and caecal microbiotas, and compared the compositions and predicted functions of microbiotas from the different intestinal segments for 20 AMEn+ and 19 AMEn- birds.
32421690	3	21	theme	bacteria	724:731	arg1	census					710:715	a more extensive census	693:715	a more extensive census of the bacteria present in the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals	693:956	Here we perform a more extensive census of the bacteria present in the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals.
32421690	4	22	theme	caecal	1017:1022	arg1	microbiotas					1024:1034	the ileal, jejunal and caecal microbiotas	994:1034	the ileal, jejunal and caecal microbiotas	994:1034	We sequenced the 16S rRNA genes of the ileal, jejunal and caecal microbiotas, and compared the compositions and predicted functions of microbiotas from the different intestinal segments for 20 AMEn+ and 19 AMEn- birds.
32421690	0	23	theme	digestive	65:73	arg1	ecosystems					85:94	their digestive microbial ecosystems	59:94	their digestive microbial ecosystems	59:94	Broilers divergently selected for digestibility differ for their digestive microbial ecosystems.
32421690	7	24	theme	acids	1591:1595	arg1	degradation					1558:1568	the degradation	1554:1568	the degradation of short chain fatty acids (SCFA) from non-starch polysaccharides	1554:1634	Furthermore, we predicted that the caecal microbiota of efficient birds might be enriched in genes contributing to the degradation of short chain fatty acids (SCFA) from non-starch polysaccharides.
32421690	2	25	theme	resulting	475:483	arg1	D+					502:503	D+	502:503	D+ (high digestive efficiency or "digestibility +")	502:552	By selecting chicken lines divergently for their digestive efficiency, we showed previously that digestive efficiency is under genetic control and that the two resulting divergent lines, D+ (high digestive efficiency or "digestibility +") and D- (low digestive efficiency or "digestibility -"), also differ for the abundance of specific bacteria in their caeca.
32421690	2	25	theme	resulting	475:483	arg1	lines					495:499	resulting divergent lines	475:499	the two resulting divergent lines	467:499	By selecting chicken lines divergently for their digestive efficiency, we showed previously that digestive efficiency is under genetic control and that the two resulting divergent lines, D+ (high digestive efficiency or "digestibility +") and D- (low digestive efficiency or "digestibility -"), also differ for the abundance of specific bacteria in their caeca.
32421690	2	25	theme	resulting	475:483	arg1	D-					558:559	D-	558:559	D- (low digestive efficiency or "digestibility -")	558:607	By selecting chicken lines divergently for their digestive efficiency, we showed previously that digestive efficiency is under genetic control and that the two resulting divergent lines, D+ (high digestive efficiency or "digestibility +") and D- (low digestive efficiency or "digestibility -"), also differ for the abundance of specific bacteria in their caeca.
32421690	2	26	theme	bacteria	652:659	arg1	abundance					630:638	the abundance	626:638	the abundance of specific bacteria in their caeca	626:674	By selecting chicken lines divergently for their digestive efficiency, we showed previously that digestive efficiency is under genetic control and that the two resulting divergent lines, D+ (high digestive efficiency or "digestibility +") and D- (low digestive efficiency or "digestibility -"), also differ for the abundance of specific bacteria in their caeca.
32421690	3	27	theme	digestive	748:756	arg1	microbiota					758:767	the digestive microbiota	744:767	the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals	744:956	Here we perform a more extensive census of the bacteria present in the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals.
32421690	8	28	theme	genetic	1677:1683	arg1	selection					1685:1693	the genetic selection	1673:1693	the genetic selection led on digestibility	1673:1714	These results confirm the impact of the genetic selection led on digestibility on the caecal microbiota taxonomic composition.
32421690	7	29	theme	chain	1579:1583	arg1	acids					1591:1595	short chain fatty acids	1573:1595	short chain fatty acids (SCFA) from non-starch polysaccharides	1573:1634	Furthermore, we predicted that the caecal microbiota of efficient birds might be enriched in genes contributing to the degradation of short chain fatty acids (SCFA) from non-starch polysaccharides.
32421690	7	29	theme	chain	1579:1583	arg1	SCFA					1598:1601	SCFA	1598:1601	SCFA	1598:1601	Furthermore, we predicted that the caecal microbiota of efficient birds might be enriched in genes contributing to the degradation of short chain fatty acids (SCFA) from non-starch polysaccharides.
32421690	2	30	theme	high	506:509	arg1	efficiency					521:530	high digestive efficiency	506:530	high digestive efficiency	506:530	By selecting chicken lines divergently for their digestive efficiency, we showed previously that digestive efficiency is under genetic control and that the two resulting divergent lines, D+ (high digestive efficiency or "digestibility +") and D- (low digestive efficiency or "digestibility -"), also differ for the abundance of specific bacteria in their caeca.
32421690	9	31	theme	taxons	1900:1905	arg1	abundances					1876:1885	the abundances	1872:1885	the abundances of bacterial taxons	1872:1905	They open the way toward the identification of specific, causal genes of the host controlling variations in the abundances of bacterial taxons.
32421690	2	32	theme	digestibility	591:603	arg1	"					606:606	"digestibility -"	590:606	"digestibility -"	590:606	By selecting chicken lines divergently for their digestive efficiency, we showed previously that digestive efficiency is under genetic control and that the two resulting divergent lines, D+ (high digestive efficiency or "digestibility +") and D- (low digestive efficiency or "digestibility -"), also differ for the abundance of specific bacteria in their caeca.
32421690	5	33	theme	origin	1204:1209	arg1	segment					1193:1199	The intestinal segment	1178:1199	The intestinal segment of origin	1178:1209	The intestinal segment of origin was the main factor structuring the samples.
32421690	5	33	theme	origin	1204:1209	arg1	factor					1224:1229	the main factor	1215:1229	the main factor structuring the samples	1215:1253	The intestinal segment of origin was the main factor structuring the samples.
32421690	3	34	theme	metabolizable	816:828	arg1	energy					830:835	their low apparent metabolizable energy	797:835	their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals	797:956	Here we perform a more extensive census of the bacteria present in the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals.
32421690	2	35	theme	genetic	442:448	arg1	control					450:456	genetic control	442:456	genetic control	442:456	By selecting chicken lines divergently for their digestive efficiency, we showed previously that digestive efficiency is under genetic control and that the two resulting divergent lines, D+ (high digestive efficiency or "digestibility +") and D- (low digestive efficiency or "digestibility -"), also differ for the abundance of specific bacteria in their caeca.
32421690	4	36	theme	predicted	1071:1079	arg1	functions					1081:1089	predicted functions	1071:1089	predicted functions	1071:1089	We sequenced the 16S rRNA genes of the ileal, jejunal and caecal microbiotas, and compared the compositions and predicted functions of microbiotas from the different intestinal segments for 20 AMEn+ and 19 AMEn- birds.
32421690	9	37	from	variations	1858:1867	arg1	abundances					1876:1885	the abundances	1872:1885	the abundances of bacterial taxons	1872:1905	They open the way toward the identification of specific, causal genes of the host controlling variations in the abundances of bacterial taxons.
32421690	2	38	theme	digestive	412:420	arg1	efficiency					422:431	digestive efficiency	412:431	digestive efficiency	412:431	By selecting chicken lines divergently for their digestive efficiency, we showed previously that digestive efficiency is under genetic control and that the two resulting divergent lines, D+ (high digestive efficiency or "digestibility +") and D- (low digestive efficiency or "digestibility -"), also differ for the abundance of specific bacteria in their caeca.
32421690	3	39	theme	nitrogen	851:858	arg1	balance					860:866	nitrogen balance	851:866	nitrogen balance (AMEn-)	851:874	Here we perform a more extensive census of the bacteria present in the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals.
32421690	3	39	theme	nitrogen	851:858	arg1	AMEn-					869:873	AMEn-	869:873	AMEn-	869:873	Here we perform a more extensive census of the bacteria present in the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals.
32421690	1	40	theme	feed	289:292	arg1	impact					279:284	the impact	275:284	the impact of feed in production costs	275:312	Improving the digestive efficiency of broiler chickens (Gallus gallus) could reduce organic waste, increase the use of alternative feed not used for human consumption and reduce the impact of feed in production costs.
32421690	4	41	from	compositions	1054:1065	arg1	segments					1136:1143	the different intestinal segments	1111:1143	the different intestinal segments	1111:1143	We sequenced the 16S rRNA genes of the ileal, jejunal and caecal microbiotas, and compared the compositions and predicted functions of microbiotas from the different intestinal segments for 20 AMEn+ and 19 AMEn- birds.
32421690	3	42	theme	high	879:882	arg1	efficiency					902:911	high (AMEn+) digestive efficiency	879:911	high (AMEn+) digestive efficiency	879:911	Here we perform a more extensive census of the bacteria present in the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals.
32421690	4	43	theme	AMEn-	1165:1169	arg1	birds					1171:1175	19 AMEn- birds	1162:1175	19 AMEn- birds	1162:1175	We sequenced the 16S rRNA genes of the ileal, jejunal and caecal microbiotas, and compared the compositions and predicted functions of microbiotas from the different intestinal segments for 20 AMEn+ and 19 AMEn- birds.
32421690	2	44	theme	chicken	328:334	arg1	lines					336:340	chicken lines	328:340	chicken lines	328:340	By selecting chicken lines divergently for their digestive efficiency, we showed previously that digestive efficiency is under genetic control and that the two resulting divergent lines, D+ (high digestive efficiency or "digestibility +") and D- (low digestive efficiency or "digestibility -"), also differ for the abundance of specific bacteria in their caeca.
32421690	1	45	theme	production	297:306	arg1	costs					308:312	production costs	297:312	production costs	297:312	Improving the digestive efficiency of broiler chickens (Gallus gallus) could reduce organic waste, increase the use of alternative feed not used for human consumption and reduce the impact of feed in production costs.
32421690	6	46	theme	bacterial	1352:1360	arg1	species					1362:1368	41 bacterial species	1349:1368	41 bacterial species with abundances differing between highly and poorly efficient birds	1349:1436	The caecal microbiota was the most impacted by the differences in digestive efficiency, with 41 bacterial species with abundances differing between highly and poorly efficient birds.
32421690	1	47	theme	alternative	216:226	arg1	feed					228:231	alternative feed	216:231	alternative feed not used for human consumption	216:262	Improving the digestive efficiency of broiler chickens (Gallus gallus) could reduce organic waste, increase the use of alternative feed not used for human consumption and reduce the impact of feed in production costs.
32421690	3	48	theme	digestive	892:900	arg1	efficiency					902:911	high (AMEn+) digestive efficiency	879:911	high (AMEn+) digestive efficiency	879:911	Here we perform a more extensive census of the bacteria present in the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals.
32421690	4	49	theme	16S	976:978	arg1	genes					985:989	the 16S rRNA genes	972:989	the 16S rRNA genes of the ileal, jejunal and caecal microbiotas	972:1034	We sequenced the 16S rRNA genes of the ileal, jejunal and caecal microbiotas, and compared the compositions and predicted functions of microbiotas from the different intestinal segments for 20 AMEn+ and 19 AMEn- birds.
32421690	3	50	theme	low	803:805	arg1	energy					830:835	their low apparent metabolizable energy	797:835	their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals	797:956	Here we perform a more extensive census of the bacteria present in the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals.
32421690	3	51	theme	AMEn+	885:889	arg1	efficiency					902:911	high (AMEn+) digestive efficiency	879:911	high (AMEn+) digestive efficiency	879:911	Here we perform a more extensive census of the bacteria present in the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals.
32421690	8	52	theme	microbiota	1730:1739	arg1	composition					1751:1761	the caecal microbiota taxonomic composition	1719:1761	the caecal microbiota taxonomic composition	1719:1761	These results confirm the impact of the genetic selection led on digestibility on the caecal microbiota taxonomic composition.
32421690	3	53	theme	[D+	918:920	arg1	progeny					931:937	a [D+ x D-] F8 progeny	916:937	a [D+ x D-] F8 progeny of 200 individuals	916:956	Here we perform a more extensive census of the bacteria present in the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals.
32421690	6	54	with	species	1362:1368	arg1	abundances					1375:1384	abundances	1375:1384	abundances differing between highly and poorly efficient birds	1375:1436	The caecal microbiota was the most impacted by the differences in digestive efficiency, with 41 bacterial species with abundances differing between highly and poorly efficient birds.
32421690	9	55	dep	specific	1811:1818	arg1	causal					1821:1826	causal	1821:1826	causal	1821:1826	They open the way toward the identification of specific, causal genes of the host controlling variations in the abundances of bacterial taxons.
32421690	1	56	from	impact	279:284	arg1	costs					308:312	production costs	297:312	production costs	297:312	Improving the digestive efficiency of broiler chickens (Gallus gallus) could reduce organic waste, increase the use of alternative feed not used for human consumption and reduce the impact of feed in production costs.
32421690	3	57	theme	F8	928:929	arg1	progeny					931:937	a [D+ x D-] F8 progeny	916:937	a [D+ x D-] F8 progeny of 200 individuals	916:956	Here we perform a more extensive census of the bacteria present in the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals.
32421690	2	58	theme	digestive	566:574	arg1	efficiency					576:585	low digestive efficiency	562:585	low digestive efficiency	562:585	By selecting chicken lines divergently for their digestive efficiency, we showed previously that digestive efficiency is under genetic control and that the two resulting divergent lines, D+ (high digestive efficiency or "digestibility +") and D- (low digestive efficiency or "digestibility -"), also differ for the abundance of specific bacteria in their caeca.
32421690	3	59	theme	D-	924:925	arg1	progeny					931:937	a [D+ x D-] F8 progeny	916:937	a [D+ x D-] F8 progeny of 200 individuals	916:956	Here we perform a more extensive census of the bacteria present in the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals.
32421690	4	60	dep	compositions	1054:1065	arg1	the					1050:1052	the	1050:1052	the	1050:1052	We sequenced the 16S rRNA genes of the ileal, jejunal and caecal microbiotas, and compared the compositions and predicted functions of microbiotas from the different intestinal segments for 20 AMEn+ and 19 AMEn- birds.
32421690	7	61	theme	birds	1505:1509	arg1	microbiota					1481:1490	the caecal microbiota	1470:1490	the caecal microbiota of efficient birds	1470:1509	Furthermore, we predicted that the caecal microbiota of efficient birds might be enriched in genes contributing to the degradation of short chain fatty acids (SCFA) from non-starch polysaccharides.
32421690	8	62	from	impact	1663:1668	arg1	composition					1751:1761	the caecal microbiota taxonomic composition	1719:1761	the caecal microbiota taxonomic composition	1719:1761	These results confirm the impact of the genetic selection led on digestibility on the caecal microbiota taxonomic composition.
32421690	2	63	theme	digestive	511:519	arg1	efficiency					521:530	high digestive efficiency	506:530	high digestive efficiency	506:530	By selecting chicken lines divergently for their digestive efficiency, we showed previously that digestive efficiency is under genetic control and that the two resulting divergent lines, D+ (high digestive efficiency or "digestibility +") and D- (low digestive efficiency or "digestibility -"), also differ for the abundance of specific bacteria in their caeca.
32421690	2	64	theme	specific	643:650	arg1	bacteria					652:659	specific bacteria	643:659	specific bacteria	643:659	By selecting chicken lines divergently for their digestive efficiency, we showed previously that digestive efficiency is under genetic control and that the two resulting divergent lines, D+ (high digestive efficiency or "digestibility +") and D- (low digestive efficiency or "digestibility -"), also differ for the abundance of specific bacteria in their caeca.
32421690	7	65	theme	caecal	1474:1479	arg1	microbiota					1481:1490	the caecal microbiota	1470:1490	the caecal microbiota of efficient birds	1470:1509	Furthermore, we predicted that the caecal microbiota of efficient birds might be enriched in genes contributing to the degradation of short chain fatty acids (SCFA) from non-starch polysaccharides.
32421690	1	66	theme	chickens	143:150	arg1	efficiency					121:130	the digestive efficiency	107:130	the digestive efficiency of broiler chickens (Gallus gallus)	107:166	Improving the digestive efficiency of broiler chickens (Gallus gallus) could reduce organic waste, increase the use of alternative feed not used for human consumption and reduce the impact of feed in production costs.
32421690	7	67	theme	non-starch	1609:1618	arg1	polysaccharides					1620:1634	non-starch polysaccharides	1609:1634	non-starch polysaccharides	1609:1634	Furthermore, we predicted that the caecal microbiota of efficient birds might be enriched in genes contributing to the degradation of short chain fatty acids (SCFA) from non-starch polysaccharides.
32421690	7	68	from	degradation	1558:1568	arg1	polysaccharides					1620:1634	non-starch polysaccharides	1609:1634	non-starch polysaccharides	1609:1634	Furthermore, we predicted that the caecal microbiota of efficient birds might be enriched in genes contributing to the degradation of short chain fatty acids (SCFA) from non-starch polysaccharides.
32421690	4	69	theme	microbiotas	1024:1034	arg1	genes					985:989	the 16S rRNA genes	972:989	the 16S rRNA genes of the ileal, jejunal and caecal microbiotas	972:1034	We sequenced the 16S rRNA genes of the ileal, jejunal and caecal microbiotas, and compared the compositions and predicted functions of microbiotas from the different intestinal segments for 20 AMEn+ and 19 AMEn- birds.
32421690	9	70	theme	specific	1811:1818	arg1	genes					1828:1832	specific, causal genes	1811:1832	specific, causal genes of the host controlling variations in the abundances of bacterial taxons	1811:1905	They open the way toward the identification of specific, causal genes of the host controlling variations in the abundances of bacterial taxons.
32421690	3	71	theme	present	733:739	arg1	bacteria					724:731	the bacteria	720:731	the bacteria present in the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals	720:956	Here we perform a more extensive census of the bacteria present in the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals.
32421690	6	72	theme	caecal	1260:1265	arg1	microbiota					1267:1276	The caecal microbiota	1256:1276	The caecal microbiota	1256:1276	The caecal microbiota was the most impacted by the differences in digestive efficiency, with 41 bacterial species with abundances differing between highly and poorly efficient birds.
32421690	1	73	theme	Gallus	153:158	arg1	chickens					143:150	broiler chickens	135:150	broiler chickens (Gallus gallus)	135:166	Improving the digestive efficiency of broiler chickens (Gallus gallus) could reduce organic waste, increase the use of alternative feed not used for human consumption and reduce the impact of feed in production costs.
32421690	1	73	theme	Gallus	153:158	arg1	gallus					160:165	Gallus gallus	153:165	Gallus gallus	153:165	Improving the digestive efficiency of broiler chickens (Gallus gallus) could reduce organic waste, increase the use of alternative feed not used for human consumption and reduce the impact of feed in production costs.
32421690	2	74	theme	divergent	485:493	arg1	D+					502:503	D+	502:503	D+ (high digestive efficiency or "digestibility +")	502:552	By selecting chicken lines divergently for their digestive efficiency, we showed previously that digestive efficiency is under genetic control and that the two resulting divergent lines, D+ (high digestive efficiency or "digestibility +") and D- (low digestive efficiency or "digestibility -"), also differ for the abundance of specific bacteria in their caeca.
32421690	2	74	theme	divergent	485:493	arg1	lines					495:499	resulting divergent lines	475:499	the two resulting divergent lines	467:499	By selecting chicken lines divergently for their digestive efficiency, we showed previously that digestive efficiency is under genetic control and that the two resulting divergent lines, D+ (high digestive efficiency or "digestibility +") and D- (low digestive efficiency or "digestibility -"), also differ for the abundance of specific bacteria in their caeca.
32421690	2	74	theme	divergent	485:493	arg1	D-					558:559	D-	558:559	D- (low digestive efficiency or "digestibility -")	558:607	By selecting chicken lines divergently for their digestive efficiency, we showed previously that digestive efficiency is under genetic control and that the two resulting divergent lines, D+ (high digestive efficiency or "digestibility +") and D- (low digestive efficiency or "digestibility -"), also differ for the abundance of specific bacteria in their caeca.
32421690	3	75	theme	individuals	946:956	arg1	progeny					931:937	a [D+ x D-] F8 progeny	916:937	a [D+ x D-] F8 progeny of 200 individuals	916:956	Here we perform a more extensive census of the bacteria present in the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals.
32421690	3	76	from	present	733:739	arg1	microbiota					758:767	the digestive microbiota	744:767	the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals	744:956	Here we perform a more extensive census of the bacteria present in the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals.
32421690	3	77	theme	apparent	807:814	arg1	energy					830:835	their low apparent metabolizable energy	797:835	their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals	797:956	Here we perform a more extensive census of the bacteria present in the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals.
32421690	9	78	theme	bacterial	1890:1898	arg1	taxons					1900:1905	bacterial taxons	1890:1905	bacterial taxons	1890:1905	They open the way toward the identification of specific, causal genes of the host controlling variations in the abundances of bacterial taxons.
32421690	7	79	theme	fatty	1585:1589	arg1	acids					1591:1595	short chain fatty acids	1573:1595	short chain fatty acids (SCFA) from non-starch polysaccharides	1573:1634	Furthermore, we predicted that the caecal microbiota of efficient birds might be enriched in genes contributing to the degradation of short chain fatty acids (SCFA) from non-starch polysaccharides.
32421690	7	79	theme	fatty	1585:1589	arg1	SCFA					1598:1601	SCFA	1598:1601	SCFA	1598:1601	Furthermore, we predicted that the caecal microbiota of efficient birds might be enriched in genes contributing to the degradation of short chain fatty acids (SCFA) from non-starch polysaccharides.
32421690	4	80	theme	intestinal	1125:1134	arg1	segments					1136:1143	the different intestinal segments	1111:1143	the different intestinal segments	1111:1143	We sequenced the 16S rRNA genes of the ileal, jejunal and caecal microbiotas, and compared the compositions and predicted functions of microbiotas from the different intestinal segments for 20 AMEn+ and 19 AMEn- birds.
32421690	3	81	attach	present	733:739	arg1	microbiota					758:767	the digestive microbiota	744:767	the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals	744:956	Here we perform a more extensive census of the bacteria present in the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals.
32421690	3	81	attach	present	733:739	arg2	bacteria					724:731	the bacteria	720:731	the bacteria present in the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals	720:956	Here we perform a more extensive census of the bacteria present in the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals.
32421690	8	82	theme	selection	1685:1693	arg1	impact					1663:1668	the impact	1659:1668	the impact of the genetic selection led on digestibility on the caecal microbiota taxonomic composition	1659:1761	These results confirm the impact of the genetic selection led on digestibility on the caecal microbiota taxonomic composition.
32421690	7	83	theme	short	1573:1577	arg1	acids					1591:1595	short chain fatty acids	1573:1595	short chain fatty acids (SCFA) from non-starch polysaccharides	1573:1634	Furthermore, we predicted that the caecal microbiota of efficient birds might be enriched in genes contributing to the degradation of short chain fatty acids (SCFA) from non-starch polysaccharides.
32421690	7	83	theme	short	1573:1577	arg1	SCFA					1598:1601	SCFA	1598:1601	SCFA	1598:1601	Furthermore, we predicted that the caecal microbiota of efficient birds might be enriched in genes contributing to the degradation of short chain fatty acids (SCFA) from non-starch polysaccharides.
32421690	4	84	theme	microbiotas	1094:1104	arg1	compositions					1054:1065	compositions	1054:1065	compositions	1054:1065	We sequenced the 16S rRNA genes of the ileal, jejunal and caecal microbiotas, and compared the compositions and predicted functions of microbiotas from the different intestinal segments for 20 AMEn+ and 19 AMEn- birds.
32421690	4	84	theme	microbiotas	1094:1104	arg1	functions					1081:1089	predicted functions	1071:1089	predicted functions	1071:1089	We sequenced the 16S rRNA genes of the ileal, jejunal and caecal microbiotas, and compared the compositions and predicted functions of microbiotas from the different intestinal segments for 20 AMEn+ and 19 AMEn- birds.
32421690	2	85	theme	digestive	364:372	arg1	efficiency					374:383	their digestive efficiency	358:383	their digestive efficiency	358:383	By selecting chicken lines divergently for their digestive efficiency, we showed previously that digestive efficiency is under genetic control and that the two resulting divergent lines, D+ (high digestive efficiency or "digestibility +") and D- (low digestive efficiency or "digestibility -"), also differ for the abundance of specific bacteria in their caeca.
32421690	2	86	dep	D+	502:503	arg1	efficiency					521:530	high digestive efficiency	506:530	high digestive efficiency	506:530	By selecting chicken lines divergently for their digestive efficiency, we showed previously that digestive efficiency is under genetic control and that the two resulting divergent lines, D+ (high digestive efficiency or "digestibility +") and D- (low digestive efficiency or "digestibility -"), also differ for the abundance of specific bacteria in their caeca.
32421690	2	86	dep	D+	502:503	arg1	digestibility					536:548	digestibility	536:548	digestibility	536:548	By selecting chicken lines divergently for their digestive efficiency, we showed previously that digestive efficiency is under genetic control and that the two resulting divergent lines, D+ (high digestive efficiency or "digestibility +") and D- (low digestive efficiency or "digestibility -"), also differ for the abundance of specific bacteria in their caeca.
32421690	4	87	theme	different	1115:1123	arg1	segments					1136:1143	the different intestinal segments	1111:1143	the different intestinal segments	1111:1143	We sequenced the 16S rRNA genes of the ileal, jejunal and caecal microbiotas, and compared the compositions and predicted functions of microbiotas from the different intestinal segments for 20 AMEn+ and 19 AMEn- birds.
32421690	1	88	theme	digestive	111:119	arg1	efficiency					121:130	the digestive efficiency	107:130	the digestive efficiency of broiler chickens (Gallus gallus)	107:166	Improving the digestive efficiency of broiler chickens (Gallus gallus) could reduce organic waste, increase the use of alternative feed not used for human consumption and reduce the impact of feed in production costs.
32421690	5	89	theme	intestinal	1182:1191	arg1	segment					1193:1199	The intestinal segment	1178:1199	The intestinal segment of origin	1178:1209	The intestinal segment of origin was the main factor structuring the samples.
32421690	5	89	theme	intestinal	1182:1191	arg1	factor					1224:1229	the main factor	1215:1229	the main factor structuring the samples	1215:1253	The intestinal segment of origin was the main factor structuring the samples.
32421690	3	90	from	microbiota	758:767	arg1	present					733:739	present	733:739	present	733:739	Here we perform a more extensive census of the bacteria present in the digestive microbiota of 60 chickens selected for their low apparent metabolizable energy corrected for nitrogen balance (AMEn-) or high (AMEn+) digestive efficiency in a [D+ x D-] F8 progeny of 200 individuals.
32421690	4	91	theme	birds	1171:1175	arg1	compositions					1054:1065	compositions	1054:1065	compositions	1054:1065	We sequenced the 16S rRNA genes of the ileal, jejunal and caecal microbiotas, and compared the compositions and predicted functions of microbiotas from the different intestinal segments for 20 AMEn+ and 19 AMEn- birds.
32421690	4	91	theme	birds	1171:1175	arg1	functions					1081:1089	predicted functions	1071:1089	predicted functions	1071:1089	We sequenced the 16S rRNA genes of the ileal, jejunal and caecal microbiotas, and compared the compositions and predicted functions of microbiotas from the different intestinal segments for 20 AMEn+ and 19 AMEn- birds.
32421690	7	92	from	polysaccharides	1620:1634	arg1	acids					1591:1595	short chain fatty acids	1573:1595	short chain fatty acids (SCFA) from non-starch polysaccharides	1573:1634	Furthermore, we predicted that the caecal microbiota of efficient birds might be enriched in genes contributing to the degradation of short chain fatty acids (SCFA) from non-starch polysaccharides.
32421690	7	92	from	polysaccharides	1620:1634	arg1	degradation					1558:1568	the degradation	1554:1568	the degradation of short chain fatty acids (SCFA) from non-starch polysaccharides	1554:1634	Furthermore, we predicted that the caecal microbiota of efficient birds might be enriched in genes contributing to the degradation of short chain fatty acids (SCFA) from non-starch polysaccharides.
32421690	7	92	from	polysaccharides	1620:1634	arg1	SCFA					1598:1601	SCFA	1598:1601	SCFA	1598:1601	Furthermore, we predicted that the caecal microbiota of efficient birds might be enriched in genes contributing to the degradation of short chain fatty acids (SCFA) from non-starch polysaccharides.
32421690	4	93	from	segments	1136:1143	arg1	microbiotas					1094:1104	microbiotas	1094:1104	microbiotas from the different intestinal segments for 20 AMEn+	1094:1156	We sequenced the 16S rRNA genes of the ileal, jejunal and caecal microbiotas, and compared the compositions and predicted functions of microbiotas from the different intestinal segments for 20 AMEn+ and 19 AMEn- birds.
32421690	4	93	from	segments	1136:1143	arg1	compositions					1054:1065	compositions	1054:1065	compositions	1054:1065	We sequenced the 16S rRNA genes of the ileal, jejunal and caecal microbiotas, and compared the compositions and predicted functions of microbiotas from the different intestinal segments for 20 AMEn+ and 19 AMEn- birds.
32421690	4	93	from	segments	1136:1143	arg1	functions					1081:1089	predicted functions	1071:1089	predicted functions	1071:1089	We sequenced the 16S rRNA genes of the ileal, jejunal and caecal microbiotas, and compared the compositions and predicted functions of microbiotas from the different intestinal segments for 20 AMEn+ and 19 AMEn- birds.
32421690	4	93	from	segments	1136:1143	arg1	birds					1171:1175	19 AMEn- birds	1162:1175	19 AMEn- birds	1162:1175	We sequenced the 16S rRNA genes of the ileal, jejunal and caecal microbiotas, and compared the compositions and predicted functions of microbiotas from the different intestinal segments for 20 AMEn+ and 19 AMEn- birds.
32421690	9	94	theme	genes	1828:1832	arg1	identification					1793:1806	the identification	1789:1806	the identification of specific, causal genes of the host controlling variations in the abundances of bacterial taxons	1789:1905	They open the way toward the identification of specific, causal genes of the host controlling variations in the abundances of bacterial taxons.
32421690	5	95	theme	main	1219:1222	arg1	segment					1193:1199	The intestinal segment	1178:1199	The intestinal segment of origin	1178:1209	The intestinal segment of origin was the main factor structuring the samples.
32421690	5	95	theme	main	1219:1222	arg1	factor					1224:1229	the main factor	1215:1229	the main factor structuring the samples	1215:1253	The intestinal segment of origin was the main factor structuring the samples.
32421690	1	96	theme	feed	228:231	arg1	use					209:211	the use	205:211	the use of alternative feed not used for human consumption	205:262	Improving the digestive efficiency of broiler chickens (Gallus gallus) could reduce organic waste, increase the use of alternative feed not used for human consumption and reduce the impact of feed in production costs.
34261322	0	0	theme	Oligosaccharides	90:105	arg1	Elucidation					75:85	De Novo Structural Elucidation	56:85	De Novo Structural Elucidation of Oligosaccharides from Polysaccharides	56:126	A Multidimensional Mass Spectrometry-Based Workflow for De Novo Structural Elucidation of Oligosaccharides from Polysaccharides.
34261322	6	1	theme	whole	1231:1235	arg1	products					1242:1249	whole food products	1231:1249	whole food products	1231:1249	The approach was then used to determine the structures of oligosaccharides derived from polysaccharide standards and whole food products.
34261322	4	2	theme	3-dimensional	653:665	arg1	analysis					778:785	a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis	651:785	a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis	651:785	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	6	3	theme	food	1237:1240	arg1	products					1242:1249	whole food products	1231:1249	whole food products	1231:1249	The approach was then used to determine the structures of oligosaccharides derived from polysaccharide standards and whole food products.
34261322	4	4	theme	chromatography-tandem	583:603	arg1	LC-MS/MS					624:631	LC-MS/MS	624:631	LC-MS/MS	624:631	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	4	4	theme	chromatography-tandem	583:603	arg1	spectrometry					610:621	liquid chromatography-tandem mass spectrometry	576:621	liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides	576:962	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	7	5	theme	LC-MS	1267:1271	arg1	workflow					1273:1280	The integrated LC-MS workflow	1252:1280	The integrated LC-MS workflow	1252:1280	The integrated LC-MS workflow will reveal the in-depth structures of oligosaccharides.
34261322	1	6	theme	biological	180:189	arg1	processes					191:199	biological processes	180:199	biological processes that are dictated by their structures	180:237	Carbohydrates play essential roles in a variety of biological processes that are dictated by their structures.
34261322	4	7	theme	liquid	576:581	arg1	LC-MS/MS					624:631	LC-MS/MS	624:631	LC-MS/MS	624:631	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	4	7	theme	liquid	576:581	arg1	spectrometry					610:621	liquid chromatography-tandem mass spectrometry	576:621	liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides	576:962	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	4	8	theme	spectrometry	894:905	arg1	analyses					922:929	two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses	822:929	two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides	822:962	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	4	9	theme	chromatography-triple	856:876	arg1	spectrometry					894:905	high performance liquid chromatography-triple quadrupole mass spectrometry	832:905	two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides	822:962	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	4	9	theme	chromatography-triple	856:876	arg1	MS					918:919	UHPLC-QqQ MS	908:919	UHPLC-QqQ MS	908:919	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	7	10	theme	in-depth	1298:1305	arg1	structures					1307:1316	the in-depth structures	1294:1316	the in-depth structures of oligosaccharides	1294:1336	The integrated LC-MS workflow will reveal the in-depth structures of oligosaccharides.
34261322	4	11	theme	mass	889:892	arg1	spectrometry					894:905	high performance liquid chromatography-triple quadrupole mass spectrometry	832:905	two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides	822:962	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	4	11	theme	mass	889:892	arg1	MS					918:919	UHPLC-QqQ MS	908:919	UHPLC-QqQ MS	908:919	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	4	12	theme	concept	667:673	arg1	analysis					778:785	a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis	651:785	a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis	651:785	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	4	13	theme	chromatography-quadrupole	704:728	arg1	spectrometry					750:761	one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry	676:761	a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis	651:785	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	4	13	theme	chromatography-quadrupole	704:728	arg1	MS					774:775	HPLC-QTOF MS	764:775	HPLC-QTOF MS	764:775	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	1	14	theme	processes	191:199	arg1	variety					169:175	a variety	167:175	a variety of biological processes that are dictated by their structures	167:237	Carbohydrates play essential roles in a variety of biological processes that are dictated by their structures.
34261322	1	14	theme	processes	191:199	arg1	processes					191:199	biological processes	180:199	biological processes that are dictated by their structures	180:237	Carbohydrates play essential roles in a variety of biological processes that are dictated by their structures.
34261322	3	15	theme	spectrometry-based	374:391	arg1	workflow					393:400	a de novo mass spectrometry-based workflow	359:400	a de novo mass spectrometry-based workflow	359:400	In this work, a de novo mass spectrometry-based workflow was developed to isolate and structurally elucidate oligosaccharides to provide sequence, monosaccharide compositions, and glycosidic linkage positions.
34261322	3	16	theme	monosaccharide	492:505	arg1	compositions					507:518	monosaccharide compositions	492:518	monosaccharide compositions	492:518	In this work, a de novo mass spectrometry-based workflow was developed to isolate and structurally elucidate oligosaccharides to provide sequence, monosaccharide compositions, and glycosidic linkage positions.
34261322	0	17	theme	Multidimensional	2:17	arg1	Workflow					43:50	A Multidimensional Mass Spectrometry-Based Workflow	0:50	A Multidimensional Mass Spectrometry-Based Workflow for De Novo Structural Elucidation of Oligosaccharides from Polysaccharides	0:126	A Multidimensional Mass Spectrometry-Based Workflow for De Novo Structural Elucidation of Oligosaccharides from Polysaccharides.
34261322	4	18	theme	linkages	1003:1010	arg1	compositions					1012:1023	linkages compositions	1003:1023	linkages compositions	1003:1023	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	4	19	theme	liquid	849:854	arg1	spectrometry					894:905	high performance liquid chromatography-triple quadrupole mass spectrometry	832:905	two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides	822:962	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	4	19	theme	liquid	849:854	arg1	MS					918:919	UHPLC-QqQ MS	908:919	UHPLC-QqQ MS	908:919	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	4	20	from	methods	640:646	arg1	analysis					778:785	a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis	651:785	a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis	651:785	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	4	21	theme	-based	633:638	arg1	methods					640:646	liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods	576:646	liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides	576:962	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	4	22	theme	performance	837:847	arg1	spectrometry					894:905	high performance liquid chromatography-triple quadrupole mass spectrometry	832:905	two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides	822:962	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	4	22	theme	performance	837:847	arg1	MS					918:919	UHPLC-QqQ MS	908:919	UHPLC-QqQ MS	908:919	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	0	23	theme	Spectrometry-Based	24:41	arg1	Workflow					43:50	A Multidimensional Mass Spectrometry-Based Workflow	0:50	A Multidimensional Mass Spectrometry-Based Workflow for De Novo Structural Elucidation of Oligosaccharides from Polysaccharides	0:126	A Multidimensional Mass Spectrometry-Based Workflow for De Novo Structural Elucidation of Oligosaccharides from Polysaccharides.
34261322	5	24	theme	maltooligosaccharide	1082:1101	arg1	standards					1103:1111	maltooligosaccharide standards	1082:1111	maltooligosaccharide standards	1082:1111	The workflow was validated by applying the procedure to maltooligosaccharide standards.
34261322	4	25	theme	quadrupole	878:887	arg1	spectrometry					894:905	high performance liquid chromatography-triple quadrupole mass spectrometry	832:905	two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides	822:962	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	4	25	theme	quadrupole	878:887	arg1	MS					918:919	UHPLC-QqQ MS	908:919	UHPLC-QqQ MS	908:919	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	4	26	theme	time-of-flight	730:743	arg1	spectrometry					750:761	one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry	676:761	a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis	651:785	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	4	26	theme	time-of-flight	730:743	arg1	MS					774:775	HPLC-QTOF MS	764:775	HPLC-QTOF MS	764:775	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	0	27	theme	Mass	19:22	arg1	Workflow					43:50	A Multidimensional Mass Spectrometry-Based Workflow	0:50	A Multidimensional Mass Spectrometry-Based Workflow for De Novo Structural Elucidation of Oligosaccharides from Polysaccharides	0:126	A Multidimensional Mass Spectrometry-Based Workflow for De Novo Structural Elucidation of Oligosaccharides from Polysaccharides.
34261322	7	28	theme	integrated	1256:1265	arg1	workflow					1273:1280	The integrated LC-MS workflow	1252:1280	The integrated LC-MS workflow	1252:1280	The integrated LC-MS workflow will reveal the in-depth structures of oligosaccharides.
34261322	6	29	theme	oligosaccharides	1172:1187	arg1	structures					1158:1167	the structures	1154:1167	the structures of oligosaccharides derived from polysaccharide standards and whole food products	1154:1249	The approach was then used to determine the structures of oligosaccharides derived from polysaccharide standards and whole food products.
34261322	4	30	theme	oligosaccharide	791:805	arg1	sequencing					807:816	oligosaccharide sequencing	791:816	oligosaccharide sequencing	791:816	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	3	31	theme	glycosidic	525:534	arg1	positions					544:552	glycosidic linkage positions	525:552	glycosidic linkage positions	525:552	In this work, a de novo mass spectrometry-based workflow was developed to isolate and structurally elucidate oligosaccharides to provide sequence, monosaccharide compositions, and glycosidic linkage positions.
34261322	3	32	dep	de	361:362	arg1	novo					364:367	novo	364:367	novo	364:367	In this work, a de novo mass spectrometry-based workflow was developed to isolate and structurally elucidate oligosaccharides to provide sequence, monosaccharide compositions, and glycosidic linkage positions.
34261322	4	33	theme	spectrometry	750:761	arg1	analysis					778:785	a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis	651:785	a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis	651:785	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	4	34	theme	spectrometry	610:621	arg1	methods					640:646	liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods	576:646	liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides	576:962	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	0	35	from	Elucidation	75:85	arg1	Polysaccharides					112:126	Polysaccharides	112:126	Polysaccharides	112:126	A Multidimensional Mass Spectrometry-Based Workflow for De Novo Structural Elucidation of Oligosaccharides from Polysaccharides.
34261322	0	36	dep	De	56:57	arg1	Novo					59:62	Novo	59:62	Novo	59:62	A Multidimensional Mass Spectrometry-Based Workflow for De Novo Structural Elucidation of Oligosaccharides from Polysaccharides.
34261322	6	37	attach	derived	1189:1195	arg1	standards					1217:1225	polysaccharide standards	1202:1225	polysaccharide standards	1202:1225	The approach was then used to determine the structures of oligosaccharides derived from polysaccharide standards and whole food products.
34261322	6	37	attach	derived	1189:1195	arg2	oligosaccharides					1172:1187	oligosaccharides	1172:1187	oligosaccharides derived from polysaccharide standards and whole food products	1172:1249	The approach was then used to determine the structures of oligosaccharides derived from polysaccharide standards and whole food products.
34261322	6	37	attach	derived	1189:1195	arg1	products					1242:1249	whole food products	1231:1249	whole food products	1231:1249	The approach was then used to determine the structures of oligosaccharides derived from polysaccharide standards and whole food products.
34261322	4	38	theme	mass	745:748	arg1	spectrometry					750:761	one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry	676:761	a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis	651:785	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	4	38	theme	mass	745:748	arg1	MS					774:775	HPLC-QTOF MS	764:775	HPLC-QTOF MS	764:775	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	7	39	theme	oligosaccharides	1321:1336	arg1	structures					1307:1316	the in-depth structures	1294:1316	the in-depth structures of oligosaccharides	1294:1336	The integrated LC-MS workflow will reveal the in-depth structures of oligosaccharides.
34261322	4	40	theme	mass	605:608	arg1	LC-MS/MS					624:631	LC-MS/MS	624:631	LC-MS/MS	624:631	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	4	40	theme	mass	605:608	arg1	spectrometry					610:621	liquid chromatography-tandem mass spectrometry	576:621	liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides	576:962	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	0	41	theme	De	56:57	arg1	Elucidation					75:85	De Novo Structural Elucidation	56:85	De Novo Structural Elucidation of Oligosaccharides from Polysaccharides	56:126	A Multidimensional Mass Spectrometry-Based Workflow for De Novo Structural Elucidation of Oligosaccharides from Polysaccharides.
34261322	1	42	theme	essential	148:156	arg1	roles					158:162	essential roles	148:162	essential roles	148:162	Carbohydrates play essential roles in a variety of biological processes that are dictated by their structures.
34261322	6	43	used	used	1136:1139	arg2	approach					1118:1125	The approach	1114:1125	The approach	1114:1125	The approach was then used to determine the structures of oligosaccharides derived from polysaccharide standards and whole food products.
34261322	4	44	theme	fractionated	934:945	arg1	oligosaccharides					947:962	fractionated oligosaccharides	934:962	fractionated oligosaccharides	934:962	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	3	45	theme	linkage	536:542	arg1	positions					544:552	glycosidic linkage positions	525:552	glycosidic linkage positions	525:552	In this work, a de novo mass spectrometry-based workflow was developed to isolate and structurally elucidate oligosaccharides to provide sequence, monosaccharide compositions, and glycosidic linkage positions.
34261322	4	46	theme	high	680:683	arg1	spectrometry					750:761	one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry	676:761	a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis	651:785	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	4	46	theme	high	680:683	arg1	MS					774:775	HPLC-QTOF MS	764:775	HPLC-QTOF MS	764:775	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	0	47	from	Polysaccharides	112:126	arg1	Oligosaccharides					90:105	Oligosaccharides	90:105	Oligosaccharides from Polysaccharides	90:126	A Multidimensional Mass Spectrometry-Based Workflow for De Novo Structural Elucidation of Oligosaccharides from Polysaccharides.
34261322	0	47	from	Polysaccharides	112:126	arg1	Elucidation					75:85	De Novo Structural Elucidation	56:85	De Novo Structural Elucidation of Oligosaccharides from Polysaccharides	56:126	A Multidimensional Mass Spectrometry-Based Workflow for De Novo Structural Elucidation of Oligosaccharides from Polysaccharides.
34261322	4	48	theme	ultra	826:830	arg1	analyses					922:929	two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses	822:929	two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides	822:962	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	4	49	theme	high	832:835	arg1	spectrometry					894:905	high performance liquid chromatography-triple quadrupole mass spectrometry	832:905	two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides	822:962	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	4	49	theme	high	832:835	arg1	MS					918:919	UHPLC-QqQ MS	908:919	UHPLC-QqQ MS	908:919	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	3	50	theme	de	361:362	arg1	workflow					393:400	a de novo mass spectrometry-based workflow	359:400	a de novo mass spectrometry-based workflow	359:400	In this work, a de novo mass spectrometry-based workflow was developed to isolate and structurally elucidate oligosaccharides to provide sequence, monosaccharide compositions, and glycosidic linkage positions.
34261322	2	51	theme	structures	282:291	arg1	characterization					249:264	characterization	249:264	characterization of carbohydrate structures	249:291	However, characterization of carbohydrate structures remains extremely difficult and generally unsolved.
34261322	4	52	from	analyses	922:929	arg1	oligosaccharides					947:962	fractionated oligosaccharides	934:962	fractionated oligosaccharides	934:962	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	4	53	theme	liquid	697:702	arg1	spectrometry					750:761	one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry	676:761	a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis	651:785	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	4	53	theme	liquid	697:702	arg1	MS					774:775	HPLC-QTOF MS	764:775	HPLC-QTOF MS	764:775	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	0	54	theme	Structural	64:73	arg1	Elucidation					75:85	De Novo Structural Elucidation	56:85	De Novo Structural Elucidation of Oligosaccharides from Polysaccharides	56:126	A Multidimensional Mass Spectrometry-Based Workflow for De Novo Structural Elucidation of Oligosaccharides from Polysaccharides.
34261322	2	55	theme	carbohydrate	269:280	arg1	structures					282:291	carbohydrate structures	269:291	carbohydrate structures	269:291	However, characterization of carbohydrate structures remains extremely difficult and generally unsolved.
34261322	6	56	theme	polysaccharide	1202:1215	arg1	standards					1217:1225	polysaccharide standards	1202:1225	polysaccharide standards	1202:1225	The approach was then used to determine the structures of oligosaccharides derived from polysaccharide standards and whole food products.
34261322	4	57	from	sequencing	807:816	arg1	oligosaccharides					947:962	fractionated oligosaccharides	934:962	fractionated oligosaccharides	934:962	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	4	58	theme	performance	685:695	arg1	spectrometry					750:761	one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry	676:761	a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis	651:785	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34261322	4	58	theme	performance	685:695	arg1	MS					774:775	HPLC-QTOF MS	764:775	HPLC-QTOF MS	764:775	The approach employs liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based methods in a 3-dimensional concept: one high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) analysis for oligosaccharide sequencing and two ultra high performance liquid chromatography-triple quadrupole mass spectrometry (UHPLC-QqQ MS) analyses on fractionated oligosaccharides to determine their monosaccharides and linkages compositions.
34062885	2	0	theme	operation	552:560	arg1	group					562:566	a sham operation group	545:566	a sham operation group up to 16 weeks post implantationem	545:601	The results of the hyaluronate containing bone substitute material (BSM) were compared to a control xenogeneic BSM of the same chemical composition and a sham operation group up to 16 weeks post implantationem.
34062885	2	0	theme	operation	552:560	arg1	composition					529:539	the same chemical composition	511:539	the same chemical composition	511:539	The results of the hyaluronate containing bone substitute material (BSM) were compared to a control xenogeneic BSM of the same chemical composition and a sham operation group up to 16 weeks post implantationem.
34062885	11	1	theme	material-associated	1945:1963	arg1	healing					1977:1983	an optimal material-associated bone tissue healing	1934:1983	an optimal material-associated bone tissue healing comparable to the control xenogeneic BSM	1934:2024	No signs of the HY as a water-binding part of the BSM were histologically detectable at any of the study time points, altogether the results of the present study show that HY allows for an optimal material-associated bone tissue healing comparable to the control xenogeneic BSM.
34062885	6	2	contain	containing	1082:1091	arg2	BSM					1093:1095	BSM	1093:1095	BSM	1093:1095	The results showed that the new hyaluronate containing BSM was gradually integrated within newly formed bone up to the end of the study that ended in a condition of complete bone defect healing.
34062885	6	2	contain	containing	1082:1091	arg1	hyaluronate					1070:1080	the new hyaluronate	1062:1080	the new hyaluronate containing BSM	1062:1095	The results showed that the new hyaluronate containing BSM was gradually integrated within newly formed bone up to the end of the study that ended in a condition of complete bone defect healing.
34062885	6	3	theme	complete	1203:1210	arg1	healing					1224:1230	complete bone defect healing	1203:1230	complete bone defect healing	1203:1230	The results showed that the new hyaluronate containing BSM was gradually integrated within newly formed bone up to the end of the study that ended in a condition of complete bone defect healing.
34062885	11	4	theme	HY	1764:1765	arg1	signs					1751:1755	No signs	1748:1755	No signs of the HY as a water-binding part of the BSM	1748:1800	No signs of the HY as a water-binding part of the BSM were histologically detectable at any of the study time points, altogether the results of the present study show that HY allows for an optimal material-associated bone tissue healing comparable to the control xenogeneic BSM.
34062885	11	5	theme	comparable	1985:1994	arg1	healing					1977:1983	an optimal material-associated bone tissue healing	1934:1983	an optimal material-associated bone tissue healing comparable to the control xenogeneic BSM	1934:2024	No signs of the HY as a water-binding part of the BSM were histologically detectable at any of the study time points, altogether the results of the present study show that HY allows for an optimal material-associated bone tissue healing comparable to the control xenogeneic BSM.
34062885	4	6	theme	calvaria	818:825	arg1	model					840:844	the calvaria implantation model	814:844	the calvaria implantation model for 2, 8, and 16 weeks post implantationem	814:887	The study included 63 male Wistar rats using the calvaria implantation model for 2, 8, and 16 weeks post implantationem.
34062885	6	7	theme	formed	1135:1140	arg1	up					1147:1148	newly formed bone up	1129:1148	newly formed bone up to the end of the study that ended in a condition of complete bone defect healing	1129:1230	The results showed that the new hyaluronate containing BSM was gradually integrated within newly formed bone up to the end of the study that ended in a condition of complete bone defect healing.
34062885	13	8	theme	tissue	2258:2263	arg1	reactions					2265:2273	the inflammatory tissue reactions	2241:2273	the inflammatory tissue reactions in both material groups and the sham operation group	2241:2326	Additionally, no differences between the inflammatory tissue reactions in both material groups and the sham operation group were found.
34062885	3	9	theme	residual	650:657	arg1	hyaluronate					659:669	the residual hyaluronate	646:669	the residual hyaluronate	646:669	A major focus of the study was to analyze the residual hyaluronate and its effects on the material-dependent healing behavior and the inflammatory tissue responses.
34062885	10	10	theme	anti-inflammatory	1650:1666	arg1	macrophages					1668:1678	pro- and anti-inflammatory macrophages	1641:1678	pro- and anti-inflammatory macrophages	1641:1678	Interestingly, no differences were found between the numbers of pro- and anti-inflammatory macrophages between the three study groups over the entire course of the study.
34062885	11	11	theme	xenogeneic	2011:2020	arg1	BSM					2022:2024	the control xenogeneic BSM	1999:2024	the control xenogeneic BSM	1999:2024	No signs of the HY as a water-binding part of the BSM were histologically detectable at any of the study time points, altogether the results of the present study show that HY allows for an optimal material-associated bone tissue healing comparable to the control xenogeneic BSM.
34062885	10	12	theme	pro-	1641:1644	arg1	macrophages					1668:1678	pro- and anti-inflammatory macrophages	1641:1678	pro- and anti-inflammatory macrophages	1641:1678	Interestingly, no differences were found between the numbers of pro- and anti-inflammatory macrophages between the three study groups over the entire course of the study.
34062885	1	13	theme	study	152:156	arg1	analyses					158:165	The present in vivo study analyses	132:165	The present in vivo study analyses	132:165	The present in vivo study analyses both the inflammatory tissue reactions and the bone healing capacity of a newly developed bone substitute material (BSM) based on xenogeneic bone substitute granules combined with hyaluronate (HY) as a water-binding molecule.
34062885	4	14	theme	Wistar	796:801	arg1	rats					803:806	63 male Wistar rats	788:806	63 male Wistar rats using the calvaria implantation model for 2, 8, and 16 weeks post implantationem	788:887	The study included 63 male Wistar rats using the calvaria implantation model for 2, 8, and 16 weeks post implantationem.
34062885	4	15	theme	implantation	827:838	arg1	model					840:844	the calvaria implantation model	814:844	the calvaria implantation model for 2, 8, and 16 weeks post implantationem	814:887	The study included 63 male Wistar rats using the calvaria implantation model for 2, 8, and 16 weeks post implantationem.
34062885	9	16	theme	quantitative	1544:1555	arg1	methods					1557:1563	qualitative and (semi-) quantitative methods	1520:1563	qualitative and (semi-) quantitative methods	1520:1563	Additionally, no differences in the (inflammatory) tissue response that was analyzed via qualitative and (semi-) quantitative methods were found.
34062885	2	17	dep	16	574:575	arg1	to					571:572	to	571:572	to	571:572	The results of the hyaluronate containing bone substitute material (BSM) were compared to a control xenogeneic BSM of the same chemical composition and a sham operation group up to 16 weeks post implantationem.
34062885	14	18	theme	bone	2493:2496	arg1	regeneration					2498:2509	bone regeneration	2493:2509	bone regeneration	2493:2509	Thus, the new hyaluronate containing xenogeneic BSM and also the control BSM have been shown to be fully biocompatible without any differences regarding bone regeneration.
34062885	11	19	from	any	1836:1838	arg1	detectable					1822:1831	detectable	1822:1831	detectable	1822:1831	No signs of the HY as a water-binding part of the BSM were histologically detectable at any of the study time points, altogether the results of the present study show that HY allows for an optimal material-associated bone tissue healing comparable to the control xenogeneic BSM.
34062885	3	20	from	hyaluronate	659:669	arg1	responses					758:766	the inflammatory tissue responses	734:766	the inflammatory tissue responses	734:766	A major focus of the study was to analyze the residual hyaluronate and its effects on the material-dependent healing behavior and the inflammatory tissue responses.
34062885	3	20	from	hyaluronate	659:669	arg1	behavior					721:728	the material-dependent healing behavior	690:728	the material-dependent healing behavior	690:728	A major focus of the study was to analyze the residual hyaluronate and its effects on the material-dependent healing behavior and the inflammatory tissue responses.
34062885	5	21	theme	analysis	1005:1012	arg1	methods					1014:1020	Established and Good Laboratory Practice (GLP)-conforming histological, histopathological, and histomorphometrical analysis methods	890:1020	methods	1014:1020	Established and Good Laboratory Practice (GLP)-conforming histological, histopathological, and histomorphometrical analysis methods were conducted.
34062885	11	22	theme	present	1896:1902	arg1	study					1904:1908	the present study	1892:1908	the present study	1892:1908	No signs of the HY as a water-binding part of the BSM were histologically detectable at any of the study time points, altogether the results of the present study show that HY allows for an optimal material-associated bone tissue healing comparable to the control xenogeneic BSM.
34062885	1	23	theme	inflammatory	176:187	arg1	reactions					196:204	inflammatory tissue reactions	176:204	inflammatory tissue reactions	176:204	The present in vivo study analyses both the inflammatory tissue reactions and the bone healing capacity of a newly developed bone substitute material (BSM) based on xenogeneic bone substitute granules combined with hyaluronate (HY) as a water-binding molecule.
34062885	2	24	theme	xenogeneic	493:502	arg1	BSM					504:506	a control xenogeneic BSM	483:506	a control xenogeneic BSM of the same chemical composition	483:539	The results of the hyaluronate containing bone substitute material (BSM) were compared to a control xenogeneic BSM of the same chemical composition and a sham operation group up to 16 weeks post implantationem.
34062885	12	25	theme	gradual	2161:2167	arg1	regeneration					2190:2201	a gradual osteoconductive bone regeneration	2159:2201	a gradual osteoconductive bone regeneration	2159:2201	The added HY seems to be degraded within a very short time period of less than 2 weeks so that the remaining BSM granules allow for a gradual osteoconductive bone regeneration.
34062885	7	26	theme	BSM	1296:1298	arg1	capacity					1272:1279	the healing capacity	1260:1279	the healing capacity of the control BSM	1260:1298	Thereby, no differences to the healing capacity of the control BSM were found.
34062885	3	27	theme	healing	713:719	arg1	behavior					721:728	the material-dependent healing behavior	690:728	the material-dependent healing behavior	690:728	A major focus of the study was to analyze the residual hyaluronate and its effects on the material-dependent healing behavior and the inflammatory tissue responses.
34062885	0	28	theme	Hyaluronic	115:124	arg1	Acid					126:129	Hyaluronic Acid	115:129	Hyaluronic Acid	115:129	In Vivo Analysis of the Biocompatibility and Bone Healing Capacity of a Novel Bone Grafting Material Combined with Hyaluronic Acid.
34062885	2	29	theme	composition	529:539	arg1	BSM					504:506	a control xenogeneic BSM	483:506	a control xenogeneic BSM of the same chemical composition	483:539	The results of the hyaluronate containing bone substitute material (BSM) were compared to a control xenogeneic BSM of the same chemical composition and a sham operation group up to 16 weeks post implantationem.
34062885	2	29	theme	composition	529:539	arg1	group					562:566	a sham operation group	545:566	a sham operation group up to 16 weeks post implantationem	545:601	The results of the hyaluronate containing bone substitute material (BSM) were compared to a control xenogeneic BSM of the same chemical composition and a sham operation group up to 16 weeks post implantationem.
34062885	2	29	theme	composition	529:539	arg1	composition					529:539	the same chemical composition	511:539	the same chemical composition	511:539	The results of the hyaluronate containing bone substitute material (BSM) were compared to a control xenogeneic BSM of the same chemical composition and a sham operation group up to 16 weeks post implantationem.
34062885	12	30	theme	bone	2185:2188	arg1	regeneration					2190:2201	a gradual osteoconductive bone regeneration	2159:2201	a gradual osteoconductive bone regeneration	2159:2201	The added HY seems to be degraded within a very short time period of less than 2 weeks so that the remaining BSM granules allow for a gradual osteoconductive bone regeneration.
34062885	13	31	theme	operation	2312:2320	arg1	group					2322:2326	the sham operation group	2303:2326	the sham operation group	2303:2326	Additionally, no differences between the inflammatory tissue reactions in both material groups and the sham operation group were found.
34062885	11	32	theme	tissue	1970:1975	arg1	healing					1977:1983	an optimal material-associated bone tissue healing	1934:1983	an optimal material-associated bone tissue healing comparable to the control xenogeneic BSM	1934:2024	No signs of the HY as a water-binding part of the BSM were histologically detectable at any of the study time points, altogether the results of the present study show that HY allows for an optimal material-associated bone tissue healing comparable to the control xenogeneic BSM.
34062885	2	33	theme	same	515:518	arg1	composition					529:539	the same chemical composition	511:539	the same chemical composition	511:539	The results of the hyaluronate containing bone substitute material (BSM) were compared to a control xenogeneic BSM of the same chemical composition and a sham operation group up to 16 weeks post implantationem.
34062885	3	34	theme	inflammatory	738:749	arg1	responses					758:766	the inflammatory tissue responses	734:766	the inflammatory tissue responses	734:766	A major focus of the study was to analyze the residual hyaluronate and its effects on the material-dependent healing behavior and the inflammatory tissue responses.
34062885	6	35	theme	defect	1217:1222	arg1	healing					1224:1230	complete bone defect healing	1203:1230	complete bone defect healing	1203:1230	The results showed that the new hyaluronate containing BSM was gradually integrated within newly formed bone up to the end of the study that ended in a condition of complete bone defect healing.
34062885	11	36	theme	bone	1965:1968	arg1	healing					1977:1983	an optimal material-associated bone tissue healing	1934:1983	an optimal material-associated bone tissue healing comparable to the control xenogeneic BSM	1934:2024	No signs of the HY as a water-binding part of the BSM were histologically detectable at any of the study time points, altogether the results of the present study show that HY allows for an optimal material-associated bone tissue healing comparable to the control xenogeneic BSM.
34062885	13	37	from	reactions	2265:2273	arg1	group					2322:2326	the sham operation group	2303:2326	the sham operation group	2303:2326	Additionally, no differences between the inflammatory tissue reactions in both material groups and the sham operation group were found.
34062885	13	37	from	reactions	2265:2273	arg1	groups					2292:2297	material groups	2283:2297	material groups	2283:2297	Additionally, no differences between the inflammatory tissue reactions in both material groups and the sham operation group were found.
34062885	5	38	theme	Laboratory	911:920	arg1	Practice					922:929	Good Laboratory Practice	906:929	Good Laboratory Practice	906:929	Established and Good Laboratory Practice (GLP)-conforming histological, histopathological, and histomorphometrical analysis methods were conducted.
34062885	11	39	theme	time	1853:1856	arg1	points					1858:1863	the study time points	1843:1863	the study time points	1843:1863	No signs of the HY as a water-binding part of the BSM were histologically detectable at any of the study time points, altogether the results of the present study show that HY allows for an optimal material-associated bone tissue healing comparable to the control xenogeneic BSM.
34062885	1	40	theme	bone	308:311	arg1	granules					324:331	xenogeneic bone substitute granules	297:331	xenogeneic bone substitute granules combined with hyaluronate (HY) as a water-binding molecule	297:390	The present in vivo study analyses both the inflammatory tissue reactions and the bone healing capacity of a newly developed bone substitute material (BSM) based on xenogeneic bone substitute granules combined with hyaluronate (HY) as a water-binding molecule.
34062885	1	41	theme	in	144:145	arg1	study					152:156	The present in vivo study	132:156	The present in vivo study analyses	132:165	The present in vivo study analyses both the inflammatory tissue reactions and the bone healing capacity of a newly developed bone substitute material (BSM) based on xenogeneic bone substitute granules combined with hyaluronate (HY) as a water-binding molecule.
34062885	9	42	from	differences	1448:1458	arg1	response					1489:1496	the (inflammatory) tissue response	1463:1496	the (inflammatory) tissue response that was analyzed via qualitative and (semi-) quantitative methods	1463:1563	Additionally, no differences in the (inflammatory) tissue response that was analyzed via qualitative and (semi-) quantitative methods were found.
34062885	8	43	theme	operation	1414:1422	arg1	group					1424:1428	the sham operation group	1405:1428	the sham operation group	1405:1428	However, the bone formation in both groups was continuously significantly higher compared to the sham operation group.
34062885	0	44	theme	Novel	72:76	arg1	Material					92:99	a Novel Bone Grafting Material	70:99	a Novel Bone Grafting Material	70:99	In Vivo Analysis of the Biocompatibility and Bone Healing Capacity of a Novel Bone Grafting Material Combined with Hyaluronic Acid.
34062885	0	45	theme	Grafting	83:90	arg1	Material					92:99	a Novel Bone Grafting Material	70:99	a Novel Bone Grafting Material	70:99	In Vivo Analysis of the Biocompatibility and Bone Healing Capacity of a Novel Bone Grafting Material Combined with Hyaluronic Acid.
34062885	5	46	theme	Good	906:909	arg1	Practice					922:929	Good Laboratory Practice	906:929	Good Laboratory Practice	906:929	Established and Good Laboratory Practice (GLP)-conforming histological, histopathological, and histomorphometrical analysis methods were conducted.
34062885	12	47	theme	weeks	2108:2112	arg1	period					2086:2091	a very short time period	2068:2091	a very short time period of less than 2 weeks so that the remaining BSM granules allow for a gradual osteoconductive bone regeneration	2068:2201	The added HY seems to be degraded within a very short time period of less than 2 weeks so that the remaining BSM granules allow for a gradual osteoconductive bone regeneration.
34062885	3	48	from	effects	679:685	arg1	responses					758:766	the inflammatory tissue responses	734:766	the inflammatory tissue responses	734:766	A major focus of the study was to analyze the residual hyaluronate and its effects on the material-dependent healing behavior and the inflammatory tissue responses.
34062885	3	48	from	effects	679:685	arg1	behavior					721:728	the material-dependent healing behavior	690:728	the material-dependent healing behavior	690:728	A major focus of the study was to analyze the residual hyaluronate and its effects on the material-dependent healing behavior and the inflammatory tissue responses.
34062885	11	49	theme	water-binding	1772:1784	arg1	part					1786:1789	a water-binding part	1770:1789	a water-binding part of the BSM	1770:1800	No signs of the HY as a water-binding part of the BSM were histologically detectable at any of the study time points, altogether the results of the present study show that HY allows for an optimal material-associated bone tissue healing comparable to the control xenogeneic BSM.
34062885	0	50	theme	In	0:1	arg1	Analysis					8:15	In Vivo Analysis	0:15	In Vivo Analysis of the Biocompatibility and Bone Healing Capacity of a Novel Bone Grafting Material	0:99	In Vivo Analysis of the Biocompatibility and Bone Healing Capacity of a Novel Bone Grafting Material Combined with Hyaluronic Acid.
34062885	1	51	theme	developed	247:255	arg1	bone					257:260	a newly developed bone	239:260	a newly developed bone	239:260	The present in vivo study analyses both the inflammatory tissue reactions and the bone healing capacity of a newly developed bone substitute material (BSM) based on xenogeneic bone substitute granules combined with hyaluronate (HY) as a water-binding molecule.
34062885	12	52	theme	short	2075:2079	arg1	period					2086:2091	a very short time period	2068:2091	a very short time period of less than 2 weeks so that the remaining BSM granules allow for a gradual osteoconductive bone regeneration	2068:2201	The added HY seems to be degraded within a very short time period of less than 2 weeks so that the remaining BSM granules allow for a gradual osteoconductive bone regeneration.
34062885	8	53	dep	higher	1386:1391	arg1	compared					1393:1400	compared	1393:1400	compared to the sham operation group	1393:1428	However, the bone formation in both groups was continuously significantly higher compared to the sham operation group.
34062885	1	54	dep	analyses	158:165	arg1	both					167:170	both	167:170	both	167:170	The present in vivo study analyses both the inflammatory tissue reactions and the bone healing capacity of a newly developed bone substitute material (BSM) based on xenogeneic bone substitute granules combined with hyaluronate (HY) as a water-binding molecule.
34062885	11	55	theme	BSM	1798:1800	arg1	part					1786:1789	a water-binding part	1770:1789	a water-binding part of the BSM	1770:1800	No signs of the HY as a water-binding part of the BSM were histologically detectable at any of the study time points, altogether the results of the present study show that HY allows for an optimal material-associated bone tissue healing comparable to the control xenogeneic BSM.
34062885	0	56	theme	Healing	50:56	arg1	Capacity					58:65	the Biocompatibility and Bone Healing Capacity	20:65	the Biocompatibility and Bone Healing Capacity of a Novel Bone Grafting Material	20:99	In Vivo Analysis of the Biocompatibility and Bone Healing Capacity of a Novel Bone Grafting Material Combined with Hyaluronic Acid.
34062885	11	57	theme	control	2003:2009	arg1	BSM					2022:2024	the control xenogeneic BSM	1999:2024	the control xenogeneic BSM	1999:2024	No signs of the HY as a water-binding part of the BSM were histologically detectable at any of the study time points, altogether the results of the present study show that HY allows for an optimal material-associated bone tissue healing comparable to the control xenogeneic BSM.
34062885	2	58	dep	material	451:458	arg1	substitute					440:449	substitute	440:449	substitute	440:449	The results of the hyaluronate containing bone substitute material (BSM) were compared to a control xenogeneic BSM of the same chemical composition and a sham operation group up to 16 weeks post implantationem.
34062885	13	59	theme	inflammatory	2245:2256	arg1	reactions					2265:2273	the inflammatory tissue reactions	2241:2273	the inflammatory tissue reactions in both material groups and the sham operation group	2241:2326	Additionally, no differences between the inflammatory tissue reactions in both material groups and the sham operation group were found.
34062885	6	60	theme	new	1066:1068	arg1	hyaluronate					1070:1080	the new hyaluronate	1062:1080	the new hyaluronate containing BSM	1062:1095	The results showed that the new hyaluronate containing BSM was gradually integrated within newly formed bone up to the end of the study that ended in a condition of complete bone defect healing.
34062885	6	61	theme	bone	1142:1145	arg1	up					1147:1148	newly formed bone up	1129:1148	newly formed bone up to the end of the study that ended in a condition of complete bone defect healing	1129:1230	The results showed that the new hyaluronate containing BSM was gradually integrated within newly formed bone up to the end of the study that ended in a condition of complete bone defect healing.
34062885	8	62	theme	bone	1325:1328	arg1	higher					1386:1391	higher	1386:1391	higher	1386:1391	However, the bone formation in both groups was continuously significantly higher compared to the sham operation group.
34062885	8	62	theme	bone	1325:1328	arg1	formation					1330:1338	the bone formation	1321:1338	the bone formation in both groups	1321:1353	However, the bone formation in both groups was continuously significantly higher compared to the sham operation group.
34062885	14	63	theme	new	2350:2352	arg1	hyaluronate					2354:2364	the new hyaluronate	2346:2364	the new hyaluronate containing xenogeneic BSM and also the control BSM	2346:2415	Thus, the new hyaluronate containing xenogeneic BSM and also the control BSM have been shown to be fully biocompatible without any differences regarding bone regeneration.
34062885	14	63	theme	new	2350:2352	arg1	biocompatible					2445:2457	biocompatible	2445:2457	biocompatible	2445:2457	Thus, the new hyaluronate containing xenogeneic BSM and also the control BSM have been shown to be fully biocompatible without any differences regarding bone regeneration.
34062885	3	64	theme	major	606:610	arg1	focus					612:616	A major focus	604:616	A major focus of the study	604:629	A major focus of the study was to analyze the residual hyaluronate and its effects on the material-dependent healing behavior and the inflammatory tissue responses.
34062885	1	65	theme	bone	214:217	arg1	capacity					227:234	the bone healing capacity	210:234	the bone healing capacity	210:234	The present in vivo study analyses both the inflammatory tissue reactions and the bone healing capacity of a newly developed bone substitute material (BSM) based on xenogeneic bone substitute granules combined with hyaluronate (HY) as a water-binding molecule.
34062885	0	66	dep	In	0:1	arg1	Vivo					3:6	Vivo	3:6	Vivo	3:6	In Vivo Analysis of the Biocompatibility and Bone Healing Capacity of a Novel Bone Grafting Material Combined with Hyaluronic Acid.
34062885	6	67	theme	bone	1212:1215	arg1	healing					1224:1230	complete bone defect healing	1203:1230	complete bone defect healing	1203:1230	The results showed that the new hyaluronate containing BSM was gradually integrated within newly formed bone up to the end of the study that ended in a condition of complete bone defect healing.
34062885	2	68	theme	sham	547:550	arg1	group					562:566	a sham operation group	545:566	a sham operation group up to 16 weeks post implantationem	545:601	The results of the hyaluronate containing bone substitute material (BSM) were compared to a control xenogeneic BSM of the same chemical composition and a sham operation group up to 16 weeks post implantationem.
34062885	2	68	theme	sham	547:550	arg1	composition					529:539	the same chemical composition	511:539	the same chemical composition	511:539	The results of the hyaluronate containing bone substitute material (BSM) were compared to a control xenogeneic BSM of the same chemical composition and a sham operation group up to 16 weeks post implantationem.
34062885	3	69	theme	study	625:629	arg1	focus					612:616	A major focus	604:616	A major focus of the study	604:629	A major focus of the study was to analyze the residual hyaluronate and its effects on the material-dependent healing behavior and the inflammatory tissue responses.
34062885	10	70	theme	macrophages	1668:1678	arg1	numbers					1630:1636	the numbers	1626:1636	the numbers of pro- and anti-inflammatory macrophages	1626:1678	Interestingly, no differences were found between the numbers of pro- and anti-inflammatory macrophages between the three study groups over the entire course of the study.
34062885	11	71	theme	study	1904:1908	arg1	results					1881:1887	the results	1877:1887	the results of the present study	1877:1908	No signs of the HY as a water-binding part of the BSM were histologically detectable at any of the study time points, altogether the results of the present study show that HY allows for an optimal material-associated bone tissue healing comparable to the control xenogeneic BSM.
34062885	2	72	contain	containing	424:433	arg2	material					451:458	bone substitute material	435:458	bone substitute material (BSM)	435:464	The results of the hyaluronate containing bone substitute material (BSM) were compared to a control xenogeneic BSM of the same chemical composition and a sham operation group up to 16 weeks post implantationem.
34062885	2	72	contain	containing	424:433	arg1	hyaluronate					412:422	the hyaluronate	408:422	the hyaluronate containing bone substitute material (BSM)	408:464	The results of the hyaluronate containing bone substitute material (BSM) were compared to a control xenogeneic BSM of the same chemical composition and a sham operation group up to 16 weeks post implantationem.
34062885	2	72	contain	containing	424:433	arg2	BSM					461:463	BSM	461:463	BSM	461:463	The results of the hyaluronate containing bone substitute material (BSM) were compared to a control xenogeneic BSM of the same chemical composition and a sham operation group up to 16 weeks post implantationem.
34062885	10	73	theme	study	1698:1702	arg1	groups					1704:1709	the three study groups	1688:1709	the three study groups over the entire course of the study	1688:1745	Interestingly, no differences were found between the numbers of pro- and anti-inflammatory macrophages between the three study groups over the entire course of the study.
34062885	2	74	dep	group	562:566	arg1	weeks					577:581	up to 16 weeks	568:581	a sham operation group up to 16 weeks post implantationem	545:601	The results of the hyaluronate containing bone substitute material (BSM) were compared to a control xenogeneic BSM of the same chemical composition and a sham operation group up to 16 weeks post implantationem.
34062885	4	75	theme	male	791:794	arg1	rats					803:806	63 male Wistar rats	788:806	63 male Wistar rats using the calvaria implantation model for 2, 8, and 16 weeks post implantationem	788:887	The study included 63 male Wistar rats using the calvaria implantation model for 2, 8, and 16 weeks post implantationem.
34062885	5	76	dep	-conforming	936:946	arg1	Practice					922:929	Good Laboratory Practice	906:929	Good Laboratory Practice	906:929	Established and Good Laboratory Practice (GLP)-conforming histological, histopathological, and histomorphometrical analysis methods were conducted.
34062885	8	77	from	formation	1330:1338	arg1	groups					1348:1353	both groups	1343:1353	both groups	1343:1353	However, the bone formation in both groups was continuously significantly higher compared to the sham operation group.
34062885	12	78	theme	osteoconductive	2169:2183	arg1	regeneration					2190:2201	a gradual osteoconductive bone regeneration	2159:2201	a gradual osteoconductive bone regeneration	2159:2201	The added HY seems to be degraded within a very short time period of less than 2 weeks so that the remaining BSM granules allow for a gradual osteoconductive bone regeneration.
34062885	10	79	theme	entire	1720:1725	arg1	course					1727:1732	the entire course	1716:1732	the entire course of the study	1716:1745	Interestingly, no differences were found between the numbers of pro- and anti-inflammatory macrophages between the three study groups over the entire course of the study.
34062885	1	80	theme	tissue	189:194	arg1	reactions					196:204	inflammatory tissue reactions	176:204	inflammatory tissue reactions	176:204	The present in vivo study analyses both the inflammatory tissue reactions and the bone healing capacity of a newly developed bone substitute material (BSM) based on xenogeneic bone substitute granules combined with hyaluronate (HY) as a water-binding molecule.
34062885	2	81	theme	control	485:491	arg1	BSM					504:506	a control xenogeneic BSM	483:506	a control xenogeneic BSM of the same chemical composition	483:539	The results of the hyaluronate containing bone substitute material (BSM) were compared to a control xenogeneic BSM of the same chemical composition and a sham operation group up to 16 weeks post implantationem.
34062885	13	82	theme	sham	2307:2310	arg1	operation					2312:2320	the sham operation	2303:2320	the sham operation group	2303:2326	Additionally, no differences between the inflammatory tissue reactions in both material groups and the sham operation group were found.
34062885	12	83	theme	remaining	2126:2134	arg1	granules					2140:2147	the remaining BSM granules	2122:2147	the remaining BSM granules	2122:2147	The added HY seems to be degraded within a very short time period of less than 2 weeks so that the remaining BSM granules allow for a gradual osteoconductive bone regeneration.
34062885	2	84	theme	chemical	520:527	arg1	composition					529:539	the same chemical composition	511:539	the same chemical composition	511:539	The results of the hyaluronate containing bone substitute material (BSM) were compared to a control xenogeneic BSM of the same chemical composition and a sham operation group up to 16 weeks post implantationem.
34062885	7	85	theme	control	1288:1294	arg1	BSM					1296:1298	the control BSM	1284:1298	the control BSM	1284:1298	Thereby, no differences to the healing capacity of the control BSM were found.
34062885	3	86	theme	material-dependent	694:711	arg1	behavior					721:728	the material-dependent healing behavior	690:728	the material-dependent healing behavior	690:728	A major focus of the study was to analyze the residual hyaluronate and its effects on the material-dependent healing behavior and the inflammatory tissue responses.
34062885	6	87	theme	healing	1224:1230	arg1	condition					1190:1198	a condition	1188:1198	a condition of complete bone defect healing	1188:1230	The results showed that the new hyaluronate containing BSM was gradually integrated within newly formed bone up to the end of the study that ended in a condition of complete bone defect healing.
34062885	1	88	theme	water-binding	369:381	arg1	molecule					383:390	a water-binding molecule	367:390	a water-binding molecule	367:390	The present in vivo study analyses both the inflammatory tissue reactions and the bone healing capacity of a newly developed bone substitute material (BSM) based on xenogeneic bone substitute granules combined with hyaluronate (HY) as a water-binding molecule.
34062885	10	89	theme	study	1741:1745	arg1	course					1727:1732	the entire course	1716:1732	the entire course of the study	1716:1745	Interestingly, no differences were found between the numbers of pro- and anti-inflammatory macrophages between the three study groups over the entire course of the study.
34062885	11	90	theme	optimal	1937:1943	arg1	healing					1977:1983	an optimal material-associated bone tissue healing	1934:1983	an optimal material-associated bone tissue healing comparable to the control xenogeneic BSM	1934:2024	No signs of the HY as a water-binding part of the BSM were histologically detectable at any of the study time points, altogether the results of the present study show that HY allows for an optimal material-associated bone tissue healing comparable to the control xenogeneic BSM.
34062885	5	91	dep	Established	890:900	arg1	histological					948:959	histological	948:959	histological	948:959	Established and Good Laboratory Practice (GLP)-conforming histological, histopathological, and histomorphometrical analysis methods were conducted.
34062885	3	92	theme	tissue	751:756	arg1	responses					758:766	the inflammatory tissue responses	734:766	the inflammatory tissue responses	734:766	A major focus of the study was to analyze the residual hyaluronate and its effects on the material-dependent healing behavior and the inflammatory tissue responses.
34062885	0	93	theme	Capacity	58:65	arg1	Analysis					8:15	In Vivo Analysis	0:15	In Vivo Analysis of the Biocompatibility and Bone Healing Capacity of a Novel Bone Grafting Material	0:99	In Vivo Analysis of the Biocompatibility and Bone Healing Capacity of a Novel Bone Grafting Material Combined with Hyaluronic Acid.
34062885	5	94	theme	histomorphometrical	985:1003	arg1	methods					1014:1020	Established and Good Laboratory Practice (GLP)-conforming histological, histopathological, and histomorphometrical analysis methods	890:1020	methods	1014:1020	Established and Good Laboratory Practice (GLP)-conforming histological, histopathological, and histomorphometrical analysis methods were conducted.
34062885	11	95	theme	study	1847:1851	arg1	points					1858:1863	the study time points	1843:1863	the study time points	1843:1863	No signs of the HY as a water-binding part of the BSM were histologically detectable at any of the study time points, altogether the results of the present study show that HY allows for an optimal material-associated bone tissue healing comparable to the control xenogeneic BSM.
34062885	8	96	theme	sham	1409:1412	arg1	group					1424:1428	the sham operation group	1405:1428	the sham operation group	1405:1428	However, the bone formation in both groups was continuously significantly higher compared to the sham operation group.
34062885	7	97	theme	healing	1264:1270	arg1	capacity					1272:1279	the healing capacity	1260:1279	the healing capacity of the control BSM	1260:1298	Thereby, no differences to the healing capacity of the control BSM were found.
34062885	1	98	theme	xenogeneic	297:306	arg1	granules					324:331	xenogeneic bone substitute granules	297:331	xenogeneic bone substitute granules combined with hyaluronate (HY) as a water-binding molecule	297:390	The present in vivo study analyses both the inflammatory tissue reactions and the bone healing capacity of a newly developed bone substitute material (BSM) based on xenogeneic bone substitute granules combined with hyaluronate (HY) as a water-binding molecule.
34062885	1	99	theme	present	136:142	arg1	study					152:156	The present in vivo study	132:156	The present in vivo study analyses	132:165	The present in vivo study analyses both the inflammatory tissue reactions and the bone healing capacity of a newly developed bone substitute material (BSM) based on xenogeneic bone substitute granules combined with hyaluronate (HY) as a water-binding molecule.
34062885	12	100	theme	BSM	2136:2138	arg1	granules					2140:2147	the remaining BSM granules	2122:2147	the remaining BSM granules	2122:2147	The added HY seems to be degraded within a very short time period of less than 2 weeks so that the remaining BSM granules allow for a gradual osteoconductive bone regeneration.
34062885	0	101	theme	Bone	78:81	arg1	Material					92:99	a Novel Bone Grafting Material	70:99	a Novel Bone Grafting Material	70:99	In Vivo Analysis of the Biocompatibility and Bone Healing Capacity of a Novel Bone Grafting Material Combined with Hyaluronic Acid.
34062885	13	102	theme	material	2283:2290	arg1	groups					2292:2297	material groups	2283:2297	material groups	2283:2297	Additionally, no differences between the inflammatory tissue reactions in both material groups and the sham operation group were found.
34062885	14	103	theme	control	2405:2411	arg1	BSM					2413:2415	the control BSM	2401:2415	the control BSM	2401:2415	Thus, the new hyaluronate containing xenogeneic BSM and also the control BSM have been shown to be fully biocompatible without any differences regarding bone regeneration.
34062885	12	104	theme	time	2081:2084	arg1	period					2086:2091	a very short time period	2068:2091	a very short time period of less than 2 weeks so that the remaining BSM granules allow for a gradual osteoconductive bone regeneration	2068:2201	The added HY seems to be degraded within a very short time period of less than 2 weeks so that the remaining BSM granules allow for a gradual osteoconductive bone regeneration.
34062885	0	105	theme	Material	92:99	arg1	Capacity					58:65	the Biocompatibility and Bone Healing Capacity	20:65	the Biocompatibility and Bone Healing Capacity of a Novel Bone Grafting Material	20:99	In Vivo Analysis of the Biocompatibility and Bone Healing Capacity of a Novel Bone Grafting Material Combined with Hyaluronic Acid.
34062885	9	106	theme	inflammatory	1468:1479	arg1	response					1489:1496	the (inflammatory) tissue response	1463:1496	the (inflammatory) tissue response that was analyzed via qualitative and (semi-) quantitative methods	1463:1563	Additionally, no differences in the (inflammatory) tissue response that was analyzed via qualitative and (semi-) quantitative methods were found.
34062885	1	107	dep	in	144:145	arg1	vivo					147:150	vivo	147:150	vivo	147:150	The present in vivo study analyses both the inflammatory tissue reactions and the bone healing capacity of a newly developed bone substitute material (BSM) based on xenogeneic bone substitute granules combined with hyaluronate (HY) as a water-binding molecule.
34062885	1	108	theme	bone	257:260	arg1	capacity					227:234	the bone healing capacity	210:234	the bone healing capacity	210:234	The present in vivo study analyses both the inflammatory tissue reactions and the bone healing capacity of a newly developed bone substitute material (BSM) based on xenogeneic bone substitute granules combined with hyaluronate (HY) as a water-binding molecule.
34062885	1	108	theme	bone	257:260	arg1	reactions					196:204	inflammatory tissue reactions	176:204	inflammatory tissue reactions	176:204	The present in vivo study analyses both the inflammatory tissue reactions and the bone healing capacity of a newly developed bone substitute material (BSM) based on xenogeneic bone substitute granules combined with hyaluronate (HY) as a water-binding molecule.
34062885	9	109	theme	tissue	1482:1487	arg1	response					1489:1496	the (inflammatory) tissue response	1463:1496	the (inflammatory) tissue response that was analyzed via qualitative and (semi-) quantitative methods	1463:1563	Additionally, no differences in the (inflammatory) tissue response that was analyzed via qualitative and (semi-) quantitative methods were found.
34062885	6	110	theme	study	1168:1172	arg1	end					1157:1159	the end	1153:1159	the end of the study that ended in a condition of complete bone defect healing	1153:1230	The results showed that the new hyaluronate containing BSM was gradually integrated within newly formed bone up to the end of the study that ended in a condition of complete bone defect healing.
34062885	0	111	theme	Biocompatibility	24:39	arg1	Capacity					58:65	the Biocompatibility and Bone Healing Capacity	20:65	the Biocompatibility and Bone Healing Capacity of a Novel Bone Grafting Material	20:99	In Vivo Analysis of the Biocompatibility and Bone Healing Capacity of a Novel Bone Grafting Material Combined with Hyaluronic Acid.
34062885	2	112	theme	bone	435:438	arg1	BSM					461:463	BSM	461:463	BSM	461:463	The results of the hyaluronate containing bone substitute material (BSM) were compared to a control xenogeneic BSM of the same chemical composition and a sham operation group up to 16 weeks post implantationem.
34062885	2	112	theme	bone	435:438	arg1	material					451:458	bone substitute material	435:458	bone substitute material (BSM)	435:464	The results of the hyaluronate containing bone substitute material (BSM) were compared to a control xenogeneic BSM of the same chemical composition and a sham operation group up to 16 weeks post implantationem.
34062885	0	113	theme	Bone	45:48	arg1	Healing					50:56	Bone Healing	45:56	Bone Healing	45:56	In Vivo Analysis of the Biocompatibility and Bone Healing Capacity of a Novel Bone Grafting Material Combined with Hyaluronic Acid.
34062885	14	114	theme	xenogeneic	2377:2386	arg1	BSM					2388:2390	xenogeneic BSM	2377:2390	xenogeneic BSM	2377:2390	Thus, the new hyaluronate containing xenogeneic BSM and also the control BSM have been shown to be fully biocompatible without any differences regarding bone regeneration.
34062885	2	115	theme	hyaluronate	412:422	arg1	results					397:403	The results	393:403	The results of the hyaluronate containing bone substitute material (BSM)	393:464	The results of the hyaluronate containing bone substitute material (BSM) were compared to a control xenogeneic BSM of the same chemical composition and a sham operation group up to 16 weeks post implantationem.
34062885	1	116	dep	granules	324:331	arg1	substitute					313:322	substitute	313:322	substitute	313:322	The present in vivo study analyses both the inflammatory tissue reactions and the bone healing capacity of a newly developed bone substitute material (BSM) based on xenogeneic bone substitute granules combined with hyaluronate (HY) as a water-binding molecule.
34062885	14	117	contain	containing	2366:2375	arg1	hyaluronate					2354:2364	the new hyaluronate	2346:2364	the new hyaluronate containing xenogeneic BSM and also the control BSM	2346:2415	Thus, the new hyaluronate containing xenogeneic BSM and also the control BSM have been shown to be fully biocompatible without any differences regarding bone regeneration.
34062885	14	117	contain	containing	2366:2375	arg2	BSM					2388:2390	xenogeneic BSM	2377:2390	xenogeneic BSM	2377:2390	Thus, the new hyaluronate containing xenogeneic BSM and also the control BSM have been shown to be fully biocompatible without any differences regarding bone regeneration.
34062885	14	117	contain	containing	2366:2375	arg1	biocompatible					2445:2457	biocompatible	2445:2457	biocompatible	2445:2457	Thus, the new hyaluronate containing xenogeneic BSM and also the control BSM have been shown to be fully biocompatible without any differences regarding bone regeneration.
34062885	14	117	contain	containing	2366:2375	arg2	BSM					2413:2415	the control BSM	2401:2415	the control BSM	2401:2415	Thus, the new hyaluronate containing xenogeneic BSM and also the control BSM have been shown to be fully biocompatible without any differences regarding bone regeneration.
34062885	9	118	theme	qualitative	1520:1530	arg1	methods					1557:1563	qualitative and (semi-) quantitative methods	1520:1563	qualitative and (semi-) quantitative methods	1520:1563	Additionally, no differences in the (inflammatory) tissue response that was analyzed via qualitative and (semi-) quantitative methods were found.
34062885	1	119	dep	reactions	196:204	arg1	the					172:174	the	172:174	the	172:174	The present in vivo study analyses both the inflammatory tissue reactions and the bone healing capacity of a newly developed bone substitute material (BSM) based on xenogeneic bone substitute granules combined with hyaluronate (HY) as a water-binding molecule.
34062885	12	120	theme	added	2031:2035	arg1	HY					2037:2038	The added HY	2027:2038	The added HY	2027:2038	The added HY seems to be degraded within a very short time period of less than 2 weeks so that the remaining BSM granules allow for a gradual osteoconductive bone regeneration.
34062885	1	121	theme	healing	219:225	arg1	capacity					227:234	the bone healing capacity	210:234	the bone healing capacity	210:234	The present in vivo study analyses both the inflammatory tissue reactions and the bone healing capacity of a newly developed bone substitute material (BSM) based on xenogeneic bone substitute granules combined with hyaluronate (HY) as a water-binding molecule.
33118923	2	0	theme	Gram-stain-positive	97:115	arg1	strain					196:201	A Gram-stain-positive, aerobic, rod-shaped, non-motile, endospore-forming and UV-resistant bacterial strain	95:201	A Gram-stain-positive, aerobic, rod-shaped, non-motile, endospore-forming and UV-resistant bacterial strain	95:201	A Gram-stain-positive, aerobic, rod-shaped, non-motile, endospore-forming and UV-resistant bacterial strain, designated strain TKL69T, was isolated from sandy soil sampled in the Taklimakan Desert.
33118923	9	1	theme	16S	750:752	arg1	sequences					764:772	16S rRNA gene sequences	750:772	16S rRNA gene sequences	750:772	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain TKL69T has the highest similarity to Salinibacillus xinjiangensis CGMCC 1.12331T (96.9 %) but belongs to an independent taxon separated from other genera of the family Bacillaceae.
33118923	5	2	theme	respiratory	435:445	arg1	MK-7					459:462	MK-7	459:462	MK-7	459:462	The only respiratory quinone was MK-7.
33118923	5	2	theme	respiratory	435:445	arg1	quinone					447:453	The only respiratory quinone	426:453	The only respiratory quinone	426:453	The only respiratory quinone was MK-7.
33118923	4	3	dep	 0	397:398	arg1	C16 					417:420	C16 	417:420	C16 	417:420	The major fatty acids were anteiso-C15 : 0, iso-C15 : 0 and C16 : 0.
33118923	4	3	dep	 0	397:398	arg1	 0					410:411	 0	410:411	 0	410:411	The major fatty acids were anteiso-C15 : 0, iso-C15 : 0 and C16 : 0.
33118923	4	3	dep	 0	397:398	arg1	 0					422:423	 0	422:423	 0	422:423	The major fatty acids were anteiso-C15 : 0, iso-C15 : 0 and C16 : 0.
33118923	7	4	theme	unidentified	592:603	arg1	phospholipid					605:616	one unidentified phospholipid	588:616	one unidentified phospholipid	588:616	Diphosphatidyl glycerol, two unidentified aminophospholipids and one unidentified phospholipid were identified as the major polar lipids.
33118923	7	4	theme	unidentified	592:603	arg1	glycerol					538:545	Diphosphatidyl glycerol	523:545	Diphosphatidyl glycerol	523:545	Diphosphatidyl glycerol, two unidentified aminophospholipids and one unidentified phospholipid were identified as the major polar lipids.
33118923	7	4	theme	unidentified	592:603	arg1	aminophospholipids					565:582	two unidentified aminophospholipids	548:582	two unidentified aminophospholipids	548:582	Diphosphatidyl glycerol, two unidentified aminophospholipids and one unidentified phospholipid were identified as the major polar lipids.
33118923	7	4	theme	unidentified	592:603	arg1	lipids					653:658	the major polar lipids	637:658	the major polar lipids	637:658	Diphosphatidyl glycerol, two unidentified aminophospholipids and one unidentified phospholipid were identified as the major polar lipids.
33118923	9	5	theme	rRNA	754:757	arg1	sequences					764:772	16S rRNA gene sequences	750:772	16S rRNA gene sequences	750:772	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain TKL69T has the highest similarity to Salinibacillus xinjiangensis CGMCC 1.12331T (96.9 %) but belongs to an independent taxon separated from other genera of the family Bacillaceae.
33118923	7	6	theme	major	641:645	arg1	glycerol					538:545	Diphosphatidyl glycerol	523:545	Diphosphatidyl glycerol	523:545	Diphosphatidyl glycerol, two unidentified aminophospholipids and one unidentified phospholipid were identified as the major polar lipids.
33118923	7	6	theme	major	641:645	arg1	phospholipid					605:616	one unidentified phospholipid	588:616	one unidentified phospholipid	588:616	Diphosphatidyl glycerol, two unidentified aminophospholipids and one unidentified phospholipid were identified as the major polar lipids.
33118923	7	6	theme	major	641:645	arg1	aminophospholipids					565:582	two unidentified aminophospholipids	548:582	two unidentified aminophospholipids	548:582	Diphosphatidyl glycerol, two unidentified aminophospholipids and one unidentified phospholipid were identified as the major polar lipids.
33118923	7	6	theme	major	641:645	arg1	lipids					653:658	the major polar lipids	637:658	the major polar lipids	637:658	Diphosphatidyl glycerol, two unidentified aminophospholipids and one unidentified phospholipid were identified as the major polar lipids.
33118923	11	7	theme	Radiobacillus	1192:1204	arg1	24779T					1239:1244	=JCM 33497T=CICC 24779T	1222:1244	=JCM 33497T=CICC 24779T	1222:1244	nov. is proposed, with the type strain being Radiobacillus deserti TKL69T (=JCM 33497T=CICC 24779T).
33118923	11	7	theme	Radiobacillus	1192:1204	arg1	strain					1179:1184	the type strain	1170:1184	the type strain	1170:1184	nov. is proposed, with the type strain being Radiobacillus deserti TKL69T (=JCM 33497T=CICC 24779T).
33118923	11	7	theme	Radiobacillus	1192:1204	arg1	TKL69T					1214:1219	Radiobacillus deserti TKL69T	1192:1219	Radiobacillus deserti TKL69T (=JCM 33497T=CICC 24779T)	1192:1245	nov. is proposed, with the type strain being Radiobacillus deserti TKL69T (=JCM 33497T=CICC 24779T).
33118923	10	8	theme	chemotaxonomic	1003:1016	arg1	analyses					1018:1025	Phylogenetic, phenotypic and chemotaxonomic analyses	974:1025	Phylogenetic, phenotypic and chemotaxonomic analyses	974:1025	Phylogenetic, phenotypic and chemotaxonomic analyses suggested that strain TKL69T represents a novel species of a new genus, for which the name Radiobacillus gen. nov., sp.
33118923	3	9	dep	%	343:343	arg1	w/v					346:348	w/v	346:348	w/v	346:348	The strain grew at 20-50 °C, pH 6-9 and with 0-12 % (w/v) NaCl.
33118923	0	10	dep	sp	33:34	arg1	nov.					27:30	Radiobacillus deserti gen. nov.	0:30	Radiobacillus deserti gen. nov.	0:30	Radiobacillus deserti gen. nov., sp.
33118923	8	11	theme	%	716:716	arg1	content					697:703	a G+C content	691:703	a G+C content of 38.5 mol%	691:716	Genomic DNA analysis revealed a G+C content of 38.5 mol%.
33118923	4	12	dep	anteiso-C15 	384:395	arg1	anteiso-C15 					384:395	anteiso-C15 	384:395	anteiso-C15 	384:395	The major fatty acids were anteiso-C15 : 0, iso-C15 : 0 and C16 : 0.
33118923	4	12	dep	anteiso-C15 	384:395	arg1	acids					373:377	The major fatty acids	357:377	The major fatty acids	357:377	The major fatty acids were anteiso-C15 : 0, iso-C15 : 0 and C16 : 0.
33118923	4	12	dep	anteiso-C15 	384:395	arg1	 0					397:398	 0	397:398	 0	397:398	The major fatty acids were anteiso-C15 : 0, iso-C15 : 0 and C16 : 0.
33118923	7	13	theme	Diphosphatidyl	523:536	arg1	glycerol					538:545	Diphosphatidyl glycerol	523:545	Diphosphatidyl glycerol	523:545	Diphosphatidyl glycerol, two unidentified aminophospholipids and one unidentified phospholipid were identified as the major polar lipids.
33118923	7	13	theme	Diphosphatidyl	523:536	arg1	phospholipid					605:616	one unidentified phospholipid	588:616	one unidentified phospholipid	588:616	Diphosphatidyl glycerol, two unidentified aminophospholipids and one unidentified phospholipid were identified as the major polar lipids.
33118923	7	13	theme	Diphosphatidyl	523:536	arg1	aminophospholipids					565:582	two unidentified aminophospholipids	548:582	two unidentified aminophospholipids	548:582	Diphosphatidyl glycerol, two unidentified aminophospholipids and one unidentified phospholipid were identified as the major polar lipids.
33118923	7	13	theme	Diphosphatidyl	523:536	arg1	lipids					653:658	the major polar lipids	637:658	the major polar lipids	637:658	Diphosphatidyl glycerol, two unidentified aminophospholipids and one unidentified phospholipid were identified as the major polar lipids.
33118923	10	14	theme	gen.	1132:1135	arg1	nov.					1137:1140	the name Radiobacillus gen. nov.	1109:1140	the name Radiobacillus gen. nov.	1109:1140	Phylogenetic, phenotypic and chemotaxonomic analyses suggested that strain TKL69T represents a novel species of a new genus, for which the name Radiobacillus gen. nov., sp.
33118923	10	15	theme	novel	1069:1073	arg1	species					1075:1081	a novel species	1067:1081	a novel species of a new genus, for which the name Radiobacillus gen. nov., sp	1067:1144	Phylogenetic, phenotypic and chemotaxonomic analyses suggested that strain TKL69T represents a novel species of a new genus, for which the name Radiobacillus gen. nov., sp.
33118923	2	16	theme	rod-shaped	127:136	arg1	strain					196:201	A Gram-stain-positive, aerobic, rod-shaped, non-motile, endospore-forming and UV-resistant bacterial strain	95:201	A Gram-stain-positive, aerobic, rod-shaped, non-motile, endospore-forming and UV-resistant bacterial strain	95:201	A Gram-stain-positive, aerobic, rod-shaped, non-motile, endospore-forming and UV-resistant bacterial strain, designated strain TKL69T, was isolated from sandy soil sampled in the Taklimakan Desert.
33118923	11	17	theme	=JCM	1222:1225	arg1	24779T					1239:1244	=JCM 33497T=CICC 24779T	1222:1244	=JCM 33497T=CICC 24779T	1222:1244	nov. is proposed, with the type strain being Radiobacillus deserti TKL69T (=JCM 33497T=CICC 24779T).
33118923	11	17	theme	=JCM	1222:1225	arg1	TKL69T					1214:1219	Radiobacillus deserti TKL69T	1192:1219	Radiobacillus deserti TKL69T (=JCM 33497T=CICC 24779T)	1192:1245	nov. is proposed, with the type strain being Radiobacillus deserti TKL69T (=JCM 33497T=CICC 24779T).
33118923	1	18	theme	UV-resistant	45:56	arg1	bacterium					58:66	a UV-resistant bacterium	43:66	a UV-resistant bacterium	43:66	nov., a UV-resistant bacterium isolated from desert soil.
33118923	1	18	theme	UV-resistant	45:56	arg1	nov.					37:40	nov.	37:40	nov.	37:40	nov., a UV-resistant bacterium isolated from desert soil.
33118923	9	19	dep	Salinibacillus	830:843	arg1	xinjiangensis					845:857	xinjiangensis	845:857	xinjiangensis	845:857	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain TKL69T has the highest similarity to Salinibacillus xinjiangensis CGMCC 1.12331T (96.9 %) but belongs to an independent taxon separated from other genera of the family Bacillaceae.
33118923	11	20	theme	33497T=CICC	1227:1237	arg1	24779T					1239:1244	=JCM 33497T=CICC 24779T	1222:1244	=JCM 33497T=CICC 24779T	1222:1244	nov. is proposed, with the type strain being Radiobacillus deserti TKL69T (=JCM 33497T=CICC 24779T).
33118923	11	20	theme	33497T=CICC	1227:1237	arg1	TKL69T					1214:1219	Radiobacillus deserti TKL69T	1192:1219	Radiobacillus deserti TKL69T (=JCM 33497T=CICC 24779T)	1192:1245	nov. is proposed, with the type strain being Radiobacillus deserti TKL69T (=JCM 33497T=CICC 24779T).
33118923	9	21	theme	independent	901:911	arg1	taxon					913:917	an independent taxon	898:917	an independent taxon separated from other genera of the family Bacillaceae	898:971	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain TKL69T has the highest similarity to Salinibacillus xinjiangensis CGMCC 1.12331T (96.9 %) but belongs to an independent taxon separated from other genera of the family Bacillaceae.
33118923	6	22	theme	meso-diaminopimelic	497:515	arg1	peptidoglycan					479:491	The cell-wall peptidoglycan	465:491	The cell-wall peptidoglycan	465:491	The cell-wall peptidoglycan was meso-diaminopimelic acid.
33118923	6	22	theme	meso-diaminopimelic	497:515	arg1	acid					517:520	meso-diaminopimelic acid	497:520	meso-diaminopimelic acid	497:520	The cell-wall peptidoglycan was meso-diaminopimelic acid.
33118923	0	23	theme	deserti	14:20	arg1	nov.					27:30	Radiobacillus deserti gen. nov.	0:30	Radiobacillus deserti gen. nov.	0:30	Radiobacillus deserti gen. nov., sp.
33118923	2	24	theme	aerobic	118:124	arg1	strain					196:201	A Gram-stain-positive, aerobic, rod-shaped, non-motile, endospore-forming and UV-resistant bacterial strain	95:201	A Gram-stain-positive, aerobic, rod-shaped, non-motile, endospore-forming and UV-resistant bacterial strain	95:201	A Gram-stain-positive, aerobic, rod-shaped, non-motile, endospore-forming and UV-resistant bacterial strain, designated strain TKL69T, was isolated from sandy soil sampled in the Taklimakan Desert.
33118923	9	25	theme	strain	786:791	arg1	TKL69T					793:798	strain TKL69T	786:798	strain TKL69T	786:798	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain TKL69T has the highest similarity to Salinibacillus xinjiangensis CGMCC 1.12331T (96.9 %) but belongs to an independent taxon separated from other genera of the family Bacillaceae.
33118923	7	26	theme	unidentified	552:563	arg1	glycerol					538:545	Diphosphatidyl glycerol	523:545	Diphosphatidyl glycerol	523:545	Diphosphatidyl glycerol, two unidentified aminophospholipids and one unidentified phospholipid were identified as the major polar lipids.
33118923	7	26	theme	unidentified	552:563	arg1	phospholipid					605:616	one unidentified phospholipid	588:616	one unidentified phospholipid	588:616	Diphosphatidyl glycerol, two unidentified aminophospholipids and one unidentified phospholipid were identified as the major polar lipids.
33118923	7	26	theme	unidentified	552:563	arg1	aminophospholipids					565:582	two unidentified aminophospholipids	548:582	two unidentified aminophospholipids	548:582	Diphosphatidyl glycerol, two unidentified aminophospholipids and one unidentified phospholipid were identified as the major polar lipids.
33118923	7	26	theme	unidentified	552:563	arg1	lipids					653:658	the major polar lipids	637:658	the major polar lipids	637:658	Diphosphatidyl glycerol, two unidentified aminophospholipids and one unidentified phospholipid were identified as the major polar lipids.
33118923	0	27	theme	Radiobacillus	0:12	arg1	nov.					27:30	Radiobacillus deserti gen. nov.	0:30	Radiobacillus deserti gen. nov.	0:30	Radiobacillus deserti gen. nov., sp.
33118923	8	28	theme	Genomic	661:667	arg1	analysis					673:680	Genomic DNA analysis	661:680	Genomic DNA analysis	661:680	Genomic DNA analysis revealed a G+C content of 38.5 mol%.
33118923	2	29	theme	UV-resistant	173:184	arg1	strain					196:201	A Gram-stain-positive, aerobic, rod-shaped, non-motile, endospore-forming and UV-resistant bacterial strain	95:201	A Gram-stain-positive, aerobic, rod-shaped, non-motile, endospore-forming and UV-resistant bacterial strain	95:201	A Gram-stain-positive, aerobic, rod-shaped, non-motile, endospore-forming and UV-resistant bacterial strain, designated strain TKL69T, was isolated from sandy soil sampled in the Taklimakan Desert.
33118923	11	30	theme	deserti	1206:1212	arg1	24779T					1239:1244	=JCM 33497T=CICC 24779T	1222:1244	=JCM 33497T=CICC 24779T	1222:1244	nov. is proposed, with the type strain being Radiobacillus deserti TKL69T (=JCM 33497T=CICC 24779T).
33118923	11	30	theme	deserti	1206:1212	arg1	strain					1179:1184	the type strain	1170:1184	the type strain	1170:1184	nov. is proposed, with the type strain being Radiobacillus deserti TKL69T (=JCM 33497T=CICC 24779T).
33118923	11	30	theme	deserti	1206:1212	arg1	TKL69T					1214:1219	Radiobacillus deserti TKL69T	1192:1219	Radiobacillus deserti TKL69T (=JCM 33497T=CICC 24779T)	1192:1245	nov. is proposed, with the type strain being Radiobacillus deserti TKL69T (=JCM 33497T=CICC 24779T).
33118923	1	31	theme	desert	82:87	arg1	soil					89:92	desert soil	82:92	desert soil	82:92	nov., a UV-resistant bacterium isolated from desert soil.
33118923	3	32	theme	0-12 	338:342	arg1	%					343:343	%	343:343	%	343:343	The strain grew at 20-50 °C, pH 6-9 and with 0-12 % (w/v) NaCl.
33118923	7	33	theme	polar	647:651	arg1	glycerol					538:545	Diphosphatidyl glycerol	523:545	Diphosphatidyl glycerol	523:545	Diphosphatidyl glycerol, two unidentified aminophospholipids and one unidentified phospholipid were identified as the major polar lipids.
33118923	7	33	theme	polar	647:651	arg1	phospholipid					605:616	one unidentified phospholipid	588:616	one unidentified phospholipid	588:616	Diphosphatidyl glycerol, two unidentified aminophospholipids and one unidentified phospholipid were identified as the major polar lipids.
33118923	7	33	theme	polar	647:651	arg1	aminophospholipids					565:582	two unidentified aminophospholipids	548:582	two unidentified aminophospholipids	548:582	Diphosphatidyl glycerol, two unidentified aminophospholipids and one unidentified phospholipid were identified as the major polar lipids.
33118923	7	33	theme	polar	647:651	arg1	lipids					653:658	the major polar lipids	637:658	the major polar lipids	637:658	Diphosphatidyl glycerol, two unidentified aminophospholipids and one unidentified phospholipid were identified as the major polar lipids.
33118923	2	34	theme	Taklimakan	274:283	arg1	Desert					285:290	the Taklimakan Desert	270:290	the Taklimakan Desert	270:290	A Gram-stain-positive, aerobic, rod-shaped, non-motile, endospore-forming and UV-resistant bacterial strain, designated strain TKL69T, was isolated from sandy soil sampled in the Taklimakan Desert.
33118923	8	35	theme	DNA	669:671	arg1	analysis					673:680	Genomic DNA analysis	661:680	Genomic DNA analysis	661:680	Genomic DNA analysis revealed a G+C content of 38.5 mol%.
33118923	9	36	theme	highest	808:814	arg1	similarity					816:825	the highest similarity	804:825	the highest similarity to Salinibacillus xinjiangensis CGMCC 1.12331T (96.9 %)	804:881	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain TKL69T has the highest similarity to Salinibacillus xinjiangensis CGMCC 1.12331T (96.9 %) but belongs to an independent taxon separated from other genera of the family Bacillaceae.
33118923	2	37	theme	endospore-forming	151:167	arg1	strain					196:201	A Gram-stain-positive, aerobic, rod-shaped, non-motile, endospore-forming and UV-resistant bacterial strain	95:201	A Gram-stain-positive, aerobic, rod-shaped, non-motile, endospore-forming and UV-resistant bacterial strain	95:201	A Gram-stain-positive, aerobic, rod-shaped, non-motile, endospore-forming and UV-resistant bacterial strain, designated strain TKL69T, was isolated from sandy soil sampled in the Taklimakan Desert.
33118923	11	38	theme	type	1174:1177	arg1	strain					1179:1184	the type strain	1170:1184	the type strain	1170:1184	nov. is proposed, with the type strain being Radiobacillus deserti TKL69T (=JCM 33497T=CICC 24779T).
33118923	11	38	theme	type	1174:1177	arg1	TKL69T					1214:1219	Radiobacillus deserti TKL69T	1192:1219	Radiobacillus deserti TKL69T (=JCM 33497T=CICC 24779T)	1192:1245	nov. is proposed, with the type strain being Radiobacillus deserti TKL69T (=JCM 33497T=CICC 24779T).
33118923	10	39	theme	Phylogenetic	974:985	arg1	analyses					1018:1025	Phylogenetic, phenotypic and chemotaxonomic analyses	974:1025	Phylogenetic, phenotypic and chemotaxonomic analyses	974:1025	Phylogenetic, phenotypic and chemotaxonomic analyses suggested that strain TKL69T represents a novel species of a new genus, for which the name Radiobacillus gen. nov., sp.
33118923	9	40	theme	other	934:938	arg1	genera					940:945	other genera	934:945	other genera of the family Bacillaceae	934:971	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain TKL69T has the highest similarity to Salinibacillus xinjiangensis CGMCC 1.12331T (96.9 %) but belongs to an independent taxon separated from other genera of the family Bacillaceae.
33118923	10	41	dep	sp	1143:1144	arg1	nov.					1137:1140	the name Radiobacillus gen. nov.	1109:1140	the name Radiobacillus gen. nov.	1109:1140	Phylogenetic, phenotypic and chemotaxonomic analyses suggested that strain TKL69T represents a novel species of a new genus, for which the name Radiobacillus gen. nov., sp.
33118923	10	42	theme	new	1088:1090	arg1	genus					1092:1096	a new genus	1086:1096	a new genus	1086:1096	Phylogenetic, phenotypic and chemotaxonomic analyses suggested that strain TKL69T represents a novel species of a new genus, for which the name Radiobacillus gen. nov., sp.
33118923	6	43	theme	cell-wall	469:477	arg1	peptidoglycan					479:491	The cell-wall peptidoglycan	465:491	The cell-wall peptidoglycan	465:491	The cell-wall peptidoglycan was meso-diaminopimelic acid.
33118923	6	43	theme	cell-wall	469:477	arg1	acid					517:520	meso-diaminopimelic acid	497:520	meso-diaminopimelic acid	497:520	The cell-wall peptidoglycan was meso-diaminopimelic acid.
33118923	10	44	theme	strain	1042:1047	arg1	TKL69T					1049:1054	strain TKL69T	1042:1054	strain TKL69T	1042:1054	Phylogenetic, phenotypic and chemotaxonomic analyses suggested that strain TKL69T represents a novel species of a new genus, for which the name Radiobacillus gen. nov., sp.
33118923	2	45	theme	non-motile	139:148	arg1	strain					196:201	A Gram-stain-positive, aerobic, rod-shaped, non-motile, endospore-forming and UV-resistant bacterial strain	95:201	A Gram-stain-positive, aerobic, rod-shaped, non-motile, endospore-forming and UV-resistant bacterial strain	95:201	A Gram-stain-positive, aerobic, rod-shaped, non-motile, endospore-forming and UV-resistant bacterial strain, designated strain TKL69T, was isolated from sandy soil sampled in the Taklimakan Desert.
33118923	2	46	theme	strain	215:220	arg1	TKL69T					222:227	strain TKL69T	215:227	strain TKL69T	215:227	A Gram-stain-positive, aerobic, rod-shaped, non-motile, endospore-forming and UV-resistant bacterial strain, designated strain TKL69T, was isolated from sandy soil sampled in the Taklimakan Desert.
33118923	10	47	theme	genus	1092:1096	arg1	species					1075:1081	a novel species	1067:1081	a novel species of a new genus, for which the name Radiobacillus gen. nov., sp	1067:1144	Phylogenetic, phenotypic and chemotaxonomic analyses suggested that strain TKL69T represents a novel species of a new genus, for which the name Radiobacillus gen. nov., sp.
33118923	9	48	theme	Salinibacillus	830:843	arg1	1.12331T					865:872	Salinibacillus xinjiangensis CGMCC 1.12331T	830:872	Salinibacillus xinjiangensis CGMCC 1.12331T (96.9 %)	830:881	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain TKL69T has the highest similarity to Salinibacillus xinjiangensis CGMCC 1.12331T (96.9 %) but belongs to an independent taxon separated from other genera of the family Bacillaceae.
33118923	9	48	theme	Salinibacillus	830:843	arg1	%					880:880	96.9 %	875:880	96.9 %	875:880	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain TKL69T has the highest similarity to Salinibacillus xinjiangensis CGMCC 1.12331T (96.9 %) but belongs to an independent taxon separated from other genera of the family Bacillaceae.
33118923	4	49	theme	major	361:365	arg1	anteiso-C15 					384:395	anteiso-C15 	384:395	anteiso-C15 	384:395	The major fatty acids were anteiso-C15 : 0, iso-C15 : 0 and C16 : 0.
33118923	4	49	theme	major	361:365	arg1	acids					373:377	The major fatty acids	357:377	The major fatty acids	357:377	The major fatty acids were anteiso-C15 : 0, iso-C15 : 0 and C16 : 0.
33118923	4	49	theme	major	361:365	arg1	 0					397:398	 0	397:398	 0	397:398	The major fatty acids were anteiso-C15 : 0, iso-C15 : 0 and C16 : 0.
33118923	10	50	theme	phenotypic	988:997	arg1	analyses					1018:1025	Phylogenetic, phenotypic and chemotaxonomic analyses	974:1025	Phylogenetic, phenotypic and chemotaxonomic analyses	974:1025	Phylogenetic, phenotypic and chemotaxonomic analyses suggested that strain TKL69T represents a novel species of a new genus, for which the name Radiobacillus gen. nov., sp.
33118923	9	51	theme	gene	759:762	arg1	sequences					764:772	16S rRNA gene sequences	750:772	16S rRNA gene sequences	750:772	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain TKL69T has the highest similarity to Salinibacillus xinjiangensis CGMCC 1.12331T (96.9 %) but belongs to an independent taxon separated from other genera of the family Bacillaceae.
33118923	8	52	theme	G+C	693:695	arg1	content					697:703	a G+C content	691:703	a G+C content of 38.5 mol%	691:716	Genomic DNA analysis revealed a G+C content of 38.5 mol%.
33118923	9	53	theme	Phylogenetic	719:730	arg1	analysis					732:739	Phylogenetic analysis	719:739	Phylogenetic analysis based on 16S rRNA gene sequences	719:772	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain TKL69T has the highest similarity to Salinibacillus xinjiangensis CGMCC 1.12331T (96.9 %) but belongs to an independent taxon separated from other genera of the family Bacillaceae.
33118923	2	54	theme	sandy	248:252	arg1	soil					254:257	sandy soil	248:257	sandy soil sampled in the Taklimakan Desert	248:290	A Gram-stain-positive, aerobic, rod-shaped, non-motile, endospore-forming and UV-resistant bacterial strain, designated strain TKL69T, was isolated from sandy soil sampled in the Taklimakan Desert.
33118923	9	55	theme	family	954:959	arg1	Bacillaceae					961:971	the family Bacillaceae	950:971	the family Bacillaceae	950:971	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain TKL69T has the highest similarity to Salinibacillus xinjiangensis CGMCC 1.12331T (96.9 %) but belongs to an independent taxon separated from other genera of the family Bacillaceae.
33118923	8	56	theme	38.5 mol	708:715	arg1	%					716:716	38.5 mol%	708:716	38.5 mol%	708:716	Genomic DNA analysis revealed a G+C content of 38.5 mol%.
33118923	9	57	theme	CGMCC	859:863	arg1	1.12331T					865:872	Salinibacillus xinjiangensis CGMCC 1.12331T	830:872	Salinibacillus xinjiangensis CGMCC 1.12331T (96.9 %)	830:881	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain TKL69T has the highest similarity to Salinibacillus xinjiangensis CGMCC 1.12331T (96.9 %) but belongs to an independent taxon separated from other genera of the family Bacillaceae.
33118923	9	57	theme	CGMCC	859:863	arg1	%					880:880	96.9 %	875:880	96.9 %	875:880	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain TKL69T has the highest similarity to Salinibacillus xinjiangensis CGMCC 1.12331T (96.9 %) but belongs to an independent taxon separated from other genera of the family Bacillaceae.
33118923	2	58	theme	bacterial	186:194	arg1	strain					196:201	A Gram-stain-positive, aerobic, rod-shaped, non-motile, endospore-forming and UV-resistant bacterial strain	95:201	A Gram-stain-positive, aerobic, rod-shaped, non-motile, endospore-forming and UV-resistant bacterial strain	95:201	A Gram-stain-positive, aerobic, rod-shaped, non-motile, endospore-forming and UV-resistant bacterial strain, designated strain TKL69T, was isolated from sandy soil sampled in the Taklimakan Desert.
33118923	9	59	contain	has	800:802	arg2	similarity					816:825	the highest similarity	804:825	the highest similarity to Salinibacillus xinjiangensis CGMCC 1.12331T (96.9 %)	804:881	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain TKL69T has the highest similarity to Salinibacillus xinjiangensis CGMCC 1.12331T (96.9 %) but belongs to an independent taxon separated from other genera of the family Bacillaceae.
33118923	9	59	contain	has	800:802	arg1	TKL69T					793:798	strain TKL69T	786:798	strain TKL69T	786:798	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain TKL69T has the highest similarity to Salinibacillus xinjiangensis CGMCC 1.12331T (96.9 %) but belongs to an independent taxon separated from other genera of the family Bacillaceae.
33118923	9	60	theme	Bacillaceae	961:971	arg1	genera					940:945	other genera	934:945	other genera of the family Bacillaceae	934:971	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain TKL69T has the highest similarity to Salinibacillus xinjiangensis CGMCC 1.12331T (96.9 %) but belongs to an independent taxon separated from other genera of the family Bacillaceae.
33118923	10	61	theme	name	1113:1116	arg1	nov.					1137:1140	the name Radiobacillus gen. nov.	1109:1140	the name Radiobacillus gen. nov.	1109:1140	Phylogenetic, phenotypic and chemotaxonomic analyses suggested that strain TKL69T represents a novel species of a new genus, for which the name Radiobacillus gen. nov., sp.
33118923	10	62	theme	Radiobacillus	1118:1130	arg1	nov.					1137:1140	the name Radiobacillus gen. nov.	1109:1140	the name Radiobacillus gen. nov.	1109:1140	Phylogenetic, phenotypic and chemotaxonomic analyses suggested that strain TKL69T represents a novel species of a new genus, for which the name Radiobacillus gen. nov., sp.
33118923	4	63	theme	fatty	367:371	arg1	anteiso-C15 					384:395	anteiso-C15 	384:395	anteiso-C15 	384:395	The major fatty acids were anteiso-C15 : 0, iso-C15 : 0 and C16 : 0.
33118923	4	63	theme	fatty	367:371	arg1	acids					373:377	The major fatty acids	357:377	The major fatty acids	357:377	The major fatty acids were anteiso-C15 : 0, iso-C15 : 0 and C16 : 0.
33118923	4	63	theme	fatty	367:371	arg1	 0					397:398	 0	397:398	 0	397:398	The major fatty acids were anteiso-C15 : 0, iso-C15 : 0 and C16 : 0.
33118923	0	64	theme	gen.	22:25	arg1	nov.					27:30	Radiobacillus deserti gen. nov.	0:30	Radiobacillus deserti gen. nov.	0:30	Radiobacillus deserti gen. nov., sp.
33118923	5	65	theme	only	430:433	arg1	MK-7					459:462	MK-7	459:462	MK-7	459:462	The only respiratory quinone was MK-7.
33118923	5	65	theme	only	430:433	arg1	quinone					447:453	The only respiratory quinone	426:453	The only respiratory quinone	426:453	The only respiratory quinone was MK-7.
33118923	3	66	theme	%	343:343	arg1	NaCl					351:354	0-12 % (w/v) NaCl	338:354	0-12 % (w/v) NaCl	338:354	The strain grew at 20-50 °C, pH 6-9 and with 0-12 % (w/v) NaCl.
33118923	2	67	attach	isolated	234:241	arg1	soil					254:257	sandy soil	248:257	sandy soil sampled in the Taklimakan Desert	248:290	A Gram-stain-positive, aerobic, rod-shaped, non-motile, endospore-forming and UV-resistant bacterial strain, designated strain TKL69T, was isolated from sandy soil sampled in the Taklimakan Desert.
33118923	2	67	attach	isolated	234:241	arg2	strain					196:201	A Gram-stain-positive, aerobic, rod-shaped, non-motile, endospore-forming and UV-resistant bacterial strain	95:201	A Gram-stain-positive, aerobic, rod-shaped, non-motile, endospore-forming and UV-resistant bacterial strain	95:201	A Gram-stain-positive, aerobic, rod-shaped, non-motile, endospore-forming and UV-resistant bacterial strain, designated strain TKL69T, was isolated from sandy soil sampled in the Taklimakan Desert.
31730034	2	0	theme	strain	100:105	arg1	actinobacterium					83:97	A novel actinobacterium	75:97	A novel actinobacterium	75:97	A novel actinobacterium, strain K3-2T, was isolated in pure culture from a thawing ancient ice wedge at Mammoth Mountain (Eastern Siberia, Russia).
31730034	2	0	theme	strain	100:105	arg1	K3-2T					107:111	strain K3-2T	100:111	strain K3-2T	100:111	A novel actinobacterium, strain K3-2T, was isolated in pure culture from a thawing ancient ice wedge at Mammoth Mountain (Eastern Siberia, Russia).
31730034	13	1	theme	arcticus	1498:1505	arg1	sp					1507:1508	the name Serinibacter arcticus sp	1476:1508	the name Serinibacter arcticus sp	1476:1508	Thus, strain K3-2T represents a novel species of the genus Serinibacter for which the name Serinibacter arcticus sp.
31730034	15	2	theme	type	1533:1536	arg1	K3-2T					1548:1552	K3-2T	1548:1552	K3-2T (DSM 103859T=VKM Ас-2719T)	1548:1579	The type strain is K3-2T (DSM 103859T=VKM Ас-2719T).
31730034	15	2	theme	type	1533:1536	arg1	strain					1538:1543	The type strain	1529:1543	The type strain	1529:1543	The type strain is K3-2T (DSM 103859T=VKM Ас-2719T).
31730034	4	3	dep	mesophilic	407:416	arg1	growth					427:432	optimum growth	419:432	optimum growth at 28 °С	419:441	Strain K3-2T was mesophilic (optimum growth at 28 °С), but capable of growing at 4 °С.
31730034	12	4	theme	S.	1338:1339	arg1	=PS-14-7T					1365:1373	=PS-14-7T	1365:1373	=PS-14-7T	1365:1373	DNA-DNA relatedness values between strain K3-2T and S. salmoneus DSM 21801T (=Kis4-28T) and S. tropicus VKPM Ac 2044T (=PS-14-7T) were 41 and 47 %.
31730034	12	4	theme	S.	1338:1339	arg1	2044T					1358:1362	S. tropicus VKPM Ac 2044T	1338:1362	S. tropicus VKPM Ac 2044T (=PS-14-7T)	1338:1374	DNA-DNA relatedness values between strain K3-2T and S. salmoneus DSM 21801T (=Kis4-28T) and S. tropicus VKPM Ac 2044T (=PS-14-7T) were 41 and 47 %.
31730034	10	5	dep	%	1068:1068	arg1	97.20 					1062:1067	97.20 	1062:1067	97.20 	1062:1067	The closest taxonomically described relatives were Serinibacter salmoneus Kis4-28T and Serinibacter tropicus PS-14-7T, with 97.20 and 97.20 % 16 s rRNA gene sequence similarity, respectively.
31730034	10	5	dep	%	1068:1068	arg1	97.20					1052:1056	97.20	1052:1056	97.20	1052:1056	The closest taxonomically described relatives were Serinibacter salmoneus Kis4-28T and Serinibacter tropicus PS-14-7T, with 97.20 and 97.20 % 16 s rRNA gene sequence similarity, respectively.
31730034	11	6	theme	strain	1195:1200	arg1	K3-2T					1202:1206	strain K3-2T	1195:1206	strain K3-2T	1195:1206	The average nucleotide identity value of the whole genome sequence between strain K3-2T and S. salmoneus Kis4-28T was 78.9 %.
31730034	5	7	theme	strain	508:513	arg1	K3-2T					515:519	strain K3-2T	508:519	strain K3-2T	508:519	The cell-wall peptidoglycan of strain K3-2T contained lysine (the diagnostic diamino acid), glutamic acid, alanine, ornithine, glycine and serine.
31730034	11	8	theme	sequence	1178:1185	arg1	%					1243:1243	78.9 %	1238:1243	78.9 %	1238:1243	The average nucleotide identity value of the whole genome sequence between strain K3-2T and S. salmoneus Kis4-28T was 78.9 %.
31730034	11	8	theme	sequence	1178:1185	arg1	value					1152:1156	The average nucleotide identity value	1120:1156	The average nucleotide identity value of the whole genome sequence between strain K3-2T and S. salmoneus Kis4-28T	1120:1232	The average nucleotide identity value of the whole genome sequence between strain K3-2T and S. salmoneus Kis4-28T was 78.9 %.
31730034	15	9	theme	DSM	1555:1557	arg1	Ас-2719T					1571:1578	DSM 103859T=VKM Ас-2719T	1555:1578	DSM 103859T=VKM Ас-2719T	1555:1578	The type strain is K3-2T (DSM 103859T=VKM Ас-2719T).
31730034	15	9	theme	DSM	1555:1557	arg1	K3-2T					1548:1552	K3-2T	1548:1552	K3-2T (DSM 103859T=VKM Ас-2719T)	1548:1579	The type strain is K3-2T (DSM 103859T=VKM Ас-2719T).
31730034	12	10	theme	Ac	1355:1356	arg1	=PS-14-7T					1365:1373	=PS-14-7T	1365:1373	=PS-14-7T	1365:1373	DNA-DNA relatedness values between strain K3-2T and S. salmoneus DSM 21801T (=Kis4-28T) and S. tropicus VKPM Ac 2044T (=PS-14-7T) were 41 and 47 %.
31730034	12	10	theme	Ac	1355:1356	arg1	2044T					1358:1362	S. tropicus VKPM Ac 2044T	1338:1362	S. tropicus VKPM Ac 2044T (=PS-14-7T)	1338:1374	DNA-DNA relatedness values between strain K3-2T and S. salmoneus DSM 21801T (=Kis4-28T) and S. tropicus VKPM Ac 2044T (=PS-14-7T) were 41 and 47 %.
31730034	10	11	theme	described	954:962	arg1	Kis4-28T					1002:1009	Kis4-28T	1002:1009	Kis4-28T	1002:1009	The closest taxonomically described relatives were Serinibacter salmoneus Kis4-28T and Serinibacter tropicus PS-14-7T, with 97.20 and 97.20 % 16 s rRNA gene sequence similarity, respectively.
31730034	10	11	theme	described	954:962	arg1	relatives					964:972	The closest taxonomically described relatives	928:972	The closest taxonomically described relatives	928:972	The closest taxonomically described relatives were Serinibacter salmoneus Kis4-28T and Serinibacter tropicus PS-14-7T, with 97.20 and 97.20 % 16 s rRNA gene sequence similarity, respectively.
31730034	4	12	from	°С	440:441	arg1	growth					427:432	optimum growth	419:432	optimum growth at 28 °С	419:441	Strain K3-2T was mesophilic (optimum growth at 28 °С), but capable of growing at 4 °С.
31730034	5	13	theme	K3-2T	515:519	arg1	peptidoglycan					491:503	The cell-wall peptidoglycan	477:503	The cell-wall peptidoglycan of strain K3-2T	477:519	The cell-wall peptidoglycan of strain K3-2T contained lysine (the diagnostic diamino acid), glutamic acid, alanine, ornithine, glycine and serine.
31730034	13	14	theme	genus	1447:1451	arg1	Serinibacter					1453:1464	the genus Serinibacter	1443:1464	the genus Serinibacter	1443:1464	Thus, strain K3-2T represents a novel species of the genus Serinibacter for which the name Serinibacter arcticus sp.
31730034	15	15	theme	103859T=VKM	1559:1569	arg1	Ас-2719T					1571:1578	DSM 103859T=VKM Ас-2719T	1555:1578	DSM 103859T=VKM Ас-2719T	1555:1578	The type strain is K3-2T (DSM 103859T=VKM Ас-2719T).
31730034	15	15	theme	103859T=VKM	1559:1569	arg1	K3-2T					1548:1552	K3-2T	1548:1552	K3-2T (DSM 103859T=VKM Ас-2719T)	1548:1579	The type strain is K3-2T (DSM 103859T=VKM Ас-2719T).
31730034	13	16	theme	Serinibacter	1453:1464	arg1	species					1432:1438	a novel species	1424:1438	a novel species of the genus Serinibacter for which the name Serinibacter arcticus sp	1424:1508	Thus, strain K3-2T represents a novel species of the genus Serinibacter for which the name Serinibacter arcticus sp.
31730034	3	17	theme	typical	300:306	arg1	structure					290:298	the fine structure typical	281:306	the fine structure typical of Gram-positive bacteria	281:332	Colonies of strain K3-2T were yellowish orange; cells had the fine structure typical of Gram-positive bacteria, were non-motile short rods and were non-spore-forming.
31730034	4	18	theme	optimum	419:425	arg1	growth					427:432	optimum growth	419:432	optimum growth at 28 °С	419:441	Strain K3-2T was mesophilic (optimum growth at 28 °С), but capable of growing at 4 °С.
31730034	5	19	theme	glutamic	569:576	arg1	acid					578:581	glutamic acid	569:581	glutamic acid	569:581	The cell-wall peptidoglycan of strain K3-2T contained lysine (the diagnostic diamino acid), glutamic acid, alanine, ornithine, glycine and serine.
31730034	5	19	theme	glutamic	569:576	arg1	lysine					531:536	lysine	531:536	lysine (the diagnostic diamino acid)	531:566	The cell-wall peptidoglycan of strain K3-2T contained lysine (the diagnostic diamino acid), glutamic acid, alanine, ornithine, glycine and serine.
31730034	1	20	theme	thawing	48:54	arg1	wedge					68:72	a thawing ancient ice wedge	46:72	a thawing ancient ice wedge	46:72	nov., isolated from a thawing ancient ice wedge.
31730034	1	21	theme	ancient	56:62	arg1	wedge					68:72	a thawing ancient ice wedge	46:72	a thawing ancient ice wedge	46:72	nov., isolated from a thawing ancient ice wedge.
31730034	2	22	dep	Siberia	205:211	arg1	Russia					214:219	Russia	214:219	Russia	214:219	A novel actinobacterium, strain K3-2T, was isolated in pure culture from a thawing ancient ice wedge at Mammoth Mountain (Eastern Siberia, Russia).
31730034	3	23	theme	fine	285:288	arg1	structure					290:298	the fine structure typical	281:306	the fine structure typical of Gram-positive bacteria	281:332	Colonies of strain K3-2T were yellowish orange; cells had the fine structure typical of Gram-positive bacteria, were non-motile short rods and were non-spore-forming.
31730034	6	24	theme	unidentified	698:709	arg1	phospholipids					711:723	three unidentified phospholipids	692:723	three unidentified phospholipids	692:723	The polar lipids were phosphatidylglycerol, lysophosphatidylserine, three unidentified phospholipids and glycolipids.
31730034	1	25	theme	ice	64:66	arg1	wedge					68:72	a thawing ancient ice wedge	46:72	a thawing ancient ice wedge	46:72	nov., isolated from a thawing ancient ice wedge.
31730034	5	26	contain	contained	521:529	arg1	peptidoglycan					491:503	The cell-wall peptidoglycan	477:503	The cell-wall peptidoglycan of strain K3-2T	477:519	The cell-wall peptidoglycan of strain K3-2T contained lysine (the diagnostic diamino acid), glutamic acid, alanine, ornithine, glycine and serine.
31730034	5	26	contain	contained	521:529	arg2	lysine					531:536	lysine	531:536	lysine (the diagnostic diamino acid)	531:566	The cell-wall peptidoglycan of strain K3-2T contained lysine (the diagnostic diamino acid), glutamic acid, alanine, ornithine, glycine and serine.
31730034	5	26	contain	contained	521:529	arg2	acid					562:565	the diagnostic diamino acid	539:565	the diagnostic diamino acid	539:565	The cell-wall peptidoglycan of strain K3-2T contained lysine (the diagnostic diamino acid), glutamic acid, alanine, ornithine, glycine and serine.
31730034	5	26	contain	contained	521:529	arg2	alanine					584:590	alanine	584:590	alanine	584:590	The cell-wall peptidoglycan of strain K3-2T contained lysine (the diagnostic diamino acid), glutamic acid, alanine, ornithine, glycine and serine.
31730034	5	26	contain	contained	521:529	arg2	serine					616:621	serine	616:621	serine	616:621	The cell-wall peptidoglycan of strain K3-2T contained lysine (the diagnostic diamino acid), glutamic acid, alanine, ornithine, glycine and serine.
31730034	5	26	contain	contained	521:529	arg2	ornithine					593:601	ornithine	593:601	ornithine	593:601	The cell-wall peptidoglycan of strain K3-2T contained lysine (the diagnostic diamino acid), glutamic acid, alanine, ornithine, glycine and serine.
31730034	5	26	contain	contained	521:529	arg2	glycine					604:610	glycine	604:610	glycine	604:610	The cell-wall peptidoglycan of strain K3-2T contained lysine (the diagnostic diamino acid), glutamic acid, alanine, ornithine, glycine and serine.
31730034	5	26	contain	contained	521:529	arg2	acid					578:581	glutamic acid	569:581	glutamic acid	569:581	The cell-wall peptidoglycan of strain K3-2T contained lysine (the diagnostic diamino acid), glutamic acid, alanine, ornithine, glycine and serine.
31730034	0	27	theme	arcticus	13:20	arg1	sp					22:23	Serinibacter arcticus sp	0:23	Serinibacter arcticus sp.	0:24	Serinibacter arcticus sp.
31730034	5	28	theme	diagnostic	543:552	arg1	acid					562:565	the diagnostic diamino acid	539:565	the diagnostic diamino acid	539:565	The cell-wall peptidoglycan of strain K3-2T contained lysine (the diagnostic diamino acid), glutamic acid, alanine, ornithine, glycine and serine.
31730034	5	28	theme	diagnostic	543:552	arg1	lysine					531:536	lysine	531:536	lysine (the diagnostic diamino acid)	531:566	The cell-wall peptidoglycan of strain K3-2T contained lysine (the diagnostic diamino acid), glutamic acid, alanine, ornithine, glycine and serine.
31730034	5	29	theme	cell-wall	481:489	arg1	peptidoglycan					491:503	The cell-wall peptidoglycan	477:503	The cell-wall peptidoglycan of strain K3-2T	477:519	The cell-wall peptidoglycan of strain K3-2T contained lysine (the diagnostic diamino acid), glutamic acid, alanine, ornithine, glycine and serine.
31730034	12	30	theme	strain	1281:1286	arg1	K3-2T					1288:1292	strain K3-2T	1281:1292	strain K3-2T	1281:1292	DNA-DNA relatedness values between strain K3-2T and S. salmoneus DSM 21801T (=Kis4-28T) and S. tropicus VKPM Ac 2044T (=PS-14-7T) were 41 and 47 %.
31730034	12	30	theme	strain	1281:1286	arg1	=Kis4-28T					1323:1331	=Kis4-28T	1323:1331	=Kis4-28T	1323:1331	DNA-DNA relatedness values between strain K3-2T and S. salmoneus DSM 21801T (=Kis4-28T) and S. tropicus VKPM Ac 2044T (=PS-14-7T) were 41 and 47 %.
31730034	2	31	from	Mountain	187:194	arg1	wedge					170:174	a thawing ancient ice wedge	148:174	a thawing ancient ice wedge at Mammoth Mountain (Eastern Siberia, Russia)	148:220	A novel actinobacterium, strain K3-2T, was isolated in pure culture from a thawing ancient ice wedge at Mammoth Mountain (Eastern Siberia, Russia).
31730034	0	32	theme	Serinibacter	0:11	arg1	sp					22:23	Serinibacter arcticus sp	0:23	Serinibacter arcticus sp.	0:24	Serinibacter arcticus sp.
31730034	5	33	theme	diamino	554:560	arg1	acid					562:565	the diagnostic diamino acid	539:565	the diagnostic diamino acid	539:565	The cell-wall peptidoglycan of strain K3-2T contained lysine (the diagnostic diamino acid), glutamic acid, alanine, ornithine, glycine and serine.
31730034	5	33	theme	diamino	554:560	arg1	lysine					531:536	lysine	531:536	lysine (the diagnostic diamino acid)	531:566	The cell-wall peptidoglycan of strain K3-2T contained lysine (the diagnostic diamino acid), glutamic acid, alanine, ornithine, glycine and serine.
31730034	7	34	theme	fatty	752:756	arg1	anteiso-C15 					769:780	anteiso-C15 	769:780	anteiso-C15 	769:780	The major fatty acids were anteiso-C15 : 0 and C16 : 0.
31730034	7	34	theme	fatty	752:756	arg1	acids					758:762	The major fatty acids	742:762	The major fatty acids	742:762	The major fatty acids were anteiso-C15 : 0 and C16 : 0.
31730034	10	35	dep	similarity	1094:1103	arg1	%					1068:1068	%	1068:1068	%	1068:1068	The closest taxonomically described relatives were Serinibacter salmoneus Kis4-28T and Serinibacter tropicus PS-14-7T, with 97.20 and 97.20 % 16 s rRNA gene sequence similarity, respectively.
31730034	11	36	theme	nucleotide	1132:1141	arg1	%					1243:1243	78.9 %	1238:1243	78.9 %	1238:1243	The average nucleotide identity value of the whole genome sequence between strain K3-2T and S. salmoneus Kis4-28T was 78.9 %.
31730034	11	36	theme	nucleotide	1132:1141	arg1	value					1152:1156	The average nucleotide identity value	1120:1156	The average nucleotide identity value of the whole genome sequence between strain K3-2T and S. salmoneus Kis4-28T	1120:1232	The average nucleotide identity value of the whole genome sequence between strain K3-2T and S. salmoneus Kis4-28T was 78.9 %.
31730034	12	37	theme	relatedness	1254:1264	arg1	values					1266:1271	DNA-DNA relatedness values	1246:1271	DNA-DNA relatedness values between strain K3-2T and S. salmoneus DSM 21801T (=Kis4-28T) and S. tropicus VKPM Ac 2044T (=PS-14-7T)	1246:1374	DNA-DNA relatedness values between strain K3-2T and S. salmoneus DSM 21801T (=Kis4-28T) and S. tropicus VKPM Ac 2044T (=PS-14-7T) were 41 and 47 %.
31730034	13	38	theme	strain	1400:1405	arg1	K3-2T					1407:1411	strain K3-2T	1400:1411	strain K3-2T	1400:1411	Thus, strain K3-2T represents a novel species of the genus Serinibacter for which the name Serinibacter arcticus sp.
31730034	3	39	theme	Gram-positive	311:323	arg1	bacteria					325:332	Gram-positive bacteria	311:332	Gram-positive bacteria	311:332	Colonies of strain K3-2T were yellowish orange; cells had the fine structure typical of Gram-positive bacteria, were non-motile short rods and were non-spore-forming.
31730034	11	40	theme	identity	1143:1150	arg1	%					1243:1243	78.9 %	1238:1243	78.9 %	1238:1243	The average nucleotide identity value of the whole genome sequence between strain K3-2T and S. salmoneus Kis4-28T was 78.9 %.
31730034	11	40	theme	identity	1143:1150	arg1	value					1152:1156	The average nucleotide identity value	1120:1156	The average nucleotide identity value of the whole genome sequence between strain K3-2T and S. salmoneus Kis4-28T	1120:1232	The average nucleotide identity value of the whole genome sequence between strain K3-2T and S. salmoneus Kis4-28T was 78.9 %.
31730034	13	41	theme	name	1480:1483	arg1	sp					1507:1508	the name Serinibacter arcticus sp	1476:1508	the name Serinibacter arcticus sp	1476:1508	Thus, strain K3-2T represents a novel species of the genus Serinibacter for which the name Serinibacter arcticus sp.
31730034	8	42	theme	only	802:805	arg1	MK-8					832:835	MK-8	832:835	MK-8(H4)	832:839	The only menaquinone detected was MK-8(H4).
31730034	8	42	theme	only	802:805	arg1	menaquinone					807:817	The only menaquinone	798:817	The only menaquinone detected	798:826	The only menaquinone detected was MK-8(H4).
31730034	13	43	theme	Serinibacter	1485:1496	arg1	sp					1507:1508	the name Serinibacter arcticus sp	1476:1508	the name Serinibacter arcticus sp	1476:1508	Thus, strain K3-2T represents a novel species of the genus Serinibacter for which the name Serinibacter arcticus sp.
31730034	3	44	theme	short	351:355	arg1	rods					357:360	non-motile short rods	340:360	non-motile short rods	340:360	Colonies of strain K3-2T were yellowish orange; cells had the fine structure typical of Gram-positive bacteria, were non-motile short rods and were non-spore-forming.
31730034	4	45	theme	Strain	390:395	arg1	K3-2T					397:401	Strain K3-2T	390:401	Strain K3-2T	390:401	Strain K3-2T was mesophilic (optimum growth at 28 °С), but capable of growing at 4 °С.
31730034	12	46	theme	DNA-DNA	1246:1252	arg1	values					1266:1271	DNA-DNA relatedness values	1246:1271	DNA-DNA relatedness values between strain K3-2T and S. salmoneus DSM 21801T (=Kis4-28T) and S. tropicus VKPM Ac 2044T (=PS-14-7T)	1246:1374	DNA-DNA relatedness values between strain K3-2T and S. salmoneus DSM 21801T (=Kis4-28T) and S. tropicus VKPM Ac 2044T (=PS-14-7T) were 41 and 47 %.
31730034	7	47	theme	major	746:750	arg1	anteiso-C15 					769:780	anteiso-C15 	769:780	anteiso-C15 	769:780	The major fatty acids were anteiso-C15 : 0 and C16 : 0.
31730034	7	47	theme	major	746:750	arg1	acids					758:762	The major fatty acids	742:762	The major fatty acids	742:762	The major fatty acids were anteiso-C15 : 0 and C16 : 0.
31730034	12	48	theme	S.	1298:1299	arg1	21801T					1315:1320	S. salmoneus DSM 21801T	1298:1320	S. salmoneus DSM 21801T	1298:1320	DNA-DNA relatedness values between strain K3-2T and S. salmoneus DSM 21801T (=Kis4-28T) and S. tropicus VKPM Ac 2044T (=PS-14-7T) were 41 and 47 %.
31730034	9	49	theme	strain	880:885	arg1	K3-2T					887:891	strain K3-2T	880:891	strain K3-2T	880:891	16S rRNA gene analysis indicated that strain K3-2T belongs to the genus Serinibacter.
31730034	2	50	theme	novel	77:81	arg1	actinobacterium					83:97	A novel actinobacterium	75:97	A novel actinobacterium	75:97	A novel actinobacterium, strain K3-2T, was isolated in pure culture from a thawing ancient ice wedge at Mammoth Mountain (Eastern Siberia, Russia).
31730034	2	50	theme	novel	77:81	arg1	K3-2T					107:111	strain K3-2T	100:111	strain K3-2T	100:111	A novel actinobacterium, strain K3-2T, was isolated in pure culture from a thawing ancient ice wedge at Mammoth Mountain (Eastern Siberia, Russia).
31730034	11	51	theme	average	1124:1130	arg1	%					1243:1243	78.9 %	1238:1243	78.9 %	1238:1243	The average nucleotide identity value of the whole genome sequence between strain K3-2T and S. salmoneus Kis4-28T was 78.9 %.
31730034	11	51	theme	average	1124:1130	arg1	value					1152:1156	The average nucleotide identity value	1120:1156	The average nucleotide identity value of the whole genome sequence between strain K3-2T and S. salmoneus Kis4-28T	1120:1232	The average nucleotide identity value of the whole genome sequence between strain K3-2T and S. salmoneus Kis4-28T was 78.9 %.
31730034	12	52	dep	S.	1298:1299	arg1	salmoneus					1301:1309	salmoneus	1301:1309	salmoneus	1301:1309	DNA-DNA relatedness values between strain K3-2T and S. salmoneus DSM 21801T (=Kis4-28T) and S. tropicus VKPM Ac 2044T (=PS-14-7T) were 41 and 47 %.
31730034	9	53	theme	16S	842:844	arg1	analysis					856:863	16S rRNA gene analysis	842:863	16S rRNA gene analysis	842:863	16S rRNA gene analysis indicated that strain K3-2T belongs to the genus Serinibacter.
31730034	12	54	theme	DSM	1311:1313	arg1	21801T					1315:1320	S. salmoneus DSM 21801T	1298:1320	S. salmoneus DSM 21801T	1298:1320	DNA-DNA relatedness values between strain K3-2T and S. salmoneus DSM 21801T (=Kis4-28T) and S. tropicus VKPM Ac 2044T (=PS-14-7T) were 41 and 47 %.
31730034	2	55	theme	pure	130:133	arg1	culture					135:141	pure culture	130:141	pure culture	130:141	A novel actinobacterium, strain K3-2T, was isolated in pure culture from a thawing ancient ice wedge at Mammoth Mountain (Eastern Siberia, Russia).
31730034	7	56	dep	anteiso-C15 	769:780	arg1	C16 					789:792	C16 	789:792	C16 	789:792	The major fatty acids were anteiso-C15 : 0 and C16 : 0.
31730034	7	56	dep	anteiso-C15 	769:780	arg1	 0					782:783	 0	782:783	 0	782:783	The major fatty acids were anteiso-C15 : 0 and C16 : 0.
31730034	2	57	attach	isolated	118:125	arg2	actinobacterium					83:97	A novel actinobacterium	75:97	A novel actinobacterium	75:97	A novel actinobacterium, strain K3-2T, was isolated in pure culture from a thawing ancient ice wedge at Mammoth Mountain (Eastern Siberia, Russia).
31730034	2	57	attach	isolated	118:125	arg2	K3-2T					107:111	strain K3-2T	100:111	strain K3-2T	100:111	A novel actinobacterium, strain K3-2T, was isolated in pure culture from a thawing ancient ice wedge at Mammoth Mountain (Eastern Siberia, Russia).
31730034	2	57	attach	isolated	118:125	arg1	wedge					170:174	a thawing ancient ice wedge	148:174	a thawing ancient ice wedge at Mammoth Mountain (Eastern Siberia, Russia)	148:220	A novel actinobacterium, strain K3-2T, was isolated in pure culture from a thawing ancient ice wedge at Mammoth Mountain (Eastern Siberia, Russia).
31730034	3	58	theme	non-motile	340:349	arg1	rods					357:360	non-motile short rods	340:360	non-motile short rods	340:360	Colonies of strain K3-2T were yellowish orange; cells had the fine structure typical of Gram-positive bacteria, were non-motile short rods and were non-spore-forming.
31730034	11	59	theme	whole	1165:1169	arg1	sequence					1178:1185	the whole genome sequence	1161:1185	the whole genome sequence between strain K3-2T and S. salmoneus Kis4-28T	1161:1232	The average nucleotide identity value of the whole genome sequence between strain K3-2T and S. salmoneus Kis4-28T was 78.9 %.
31730034	10	60	theme	closest	932:938	arg1	Kis4-28T					1002:1009	Kis4-28T	1002:1009	Kis4-28T	1002:1009	The closest taxonomically described relatives were Serinibacter salmoneus Kis4-28T and Serinibacter tropicus PS-14-7T, with 97.20 and 97.20 % 16 s rRNA gene sequence similarity, respectively.
31730034	10	60	theme	closest	932:938	arg1	relatives					964:972	The closest taxonomically described relatives	928:972	The closest taxonomically described relatives	928:972	The closest taxonomically described relatives were Serinibacter salmoneus Kis4-28T and Serinibacter tropicus PS-14-7T, with 97.20 and 97.20 % 16 s rRNA gene sequence similarity, respectively.
31730034	11	61	theme	genome	1171:1176	arg1	sequence					1178:1185	the whole genome sequence	1161:1185	the whole genome sequence between strain K3-2T and S. salmoneus Kis4-28T	1161:1232	The average nucleotide identity value of the whole genome sequence between strain K3-2T and S. salmoneus Kis4-28T was 78.9 %.
31730034	13	62	theme	novel	1426:1430	arg1	species					1432:1438	a novel species	1424:1438	a novel species of the genus Serinibacter for which the name Serinibacter arcticus sp	1424:1508	Thus, strain K3-2T represents a novel species of the genus Serinibacter for which the name Serinibacter arcticus sp.
31730034	3	63	theme	strain	235:240	arg1	K3-2T					242:246	strain K3-2T	235:246	strain K3-2T	235:246	Colonies of strain K3-2T were yellowish orange; cells had the fine structure typical of Gram-positive bacteria, were non-motile short rods and were non-spore-forming.
31730034	2	64	theme	ice	166:168	arg1	wedge					170:174	a thawing ancient ice wedge	148:174	a thawing ancient ice wedge at Mammoth Mountain (Eastern Siberia, Russia)	148:220	A novel actinobacterium, strain K3-2T, was isolated in pure culture from a thawing ancient ice wedge at Mammoth Mountain (Eastern Siberia, Russia).
31730034	7	65	dep	 0	782:783	arg1	 0					794:795	 0	794:795	 0	794:795	The major fatty acids were anteiso-C15 : 0 and C16 : 0.
31730034	12	66	theme	VKPM	1350:1353	arg1	=PS-14-7T					1365:1373	=PS-14-7T	1365:1373	=PS-14-7T	1365:1373	DNA-DNA relatedness values between strain K3-2T and S. salmoneus DSM 21801T (=Kis4-28T) and S. tropicus VKPM Ac 2044T (=PS-14-7T) were 41 and 47 %.
31730034	12	66	theme	VKPM	1350:1353	arg1	2044T					1358:1362	S. tropicus VKPM Ac 2044T	1338:1362	S. tropicus VKPM Ac 2044T (=PS-14-7T)	1338:1374	DNA-DNA relatedness values between strain K3-2T and S. salmoneus DSM 21801T (=Kis4-28T) and S. tropicus VKPM Ac 2044T (=PS-14-7T) were 41 and 47 %.
31730034	10	67	theme	sequence	1085:1092	arg1	similarity					1094:1103	97.20 and 97.20 % 16 s rRNA gene sequence similarity	1052:1103	97.20 and 97.20 % 16 s rRNA gene sequence similarity	1052:1103	The closest taxonomically described relatives were Serinibacter salmoneus Kis4-28T and Serinibacter tropicus PS-14-7T, with 97.20 and 97.20 % 16 s rRNA gene sequence similarity, respectively.
31730034	3	68	theme	K3-2T	242:246	arg1	Colonies					223:230	Colonies	223:230	Colonies of strain K3-2T	223:246	Colonies of strain K3-2T were yellowish orange; cells had the fine structure typical of Gram-positive bacteria, were non-motile short rods and were non-spore-forming.
31730034	2	69	theme	ancient	158:164	arg1	wedge					170:174	a thawing ancient ice wedge	148:174	a thawing ancient ice wedge at Mammoth Mountain (Eastern Siberia, Russia)	148:220	A novel actinobacterium, strain K3-2T, was isolated in pure culture from a thawing ancient ice wedge at Mammoth Mountain (Eastern Siberia, Russia).
31730034	6	70	theme	polar	628:632	arg1	phosphatidylglycerol					646:665	phosphatidylglycerol	646:665	phosphatidylglycerol	646:665	The polar lipids were phosphatidylglycerol, lysophosphatidylserine, three unidentified phospholipids and glycolipids.
31730034	6	70	theme	polar	628:632	arg1	lipids					634:639	The polar lipids	624:639	The polar lipids	624:639	The polar lipids were phosphatidylglycerol, lysophosphatidylserine, three unidentified phospholipids and glycolipids.
31730034	9	71	theme	genus	908:912	arg1	Serinibacter					914:925	the genus Serinibacter	904:925	the genus Serinibacter	904:925	16S rRNA gene analysis indicated that strain K3-2T belongs to the genus Serinibacter.
31730034	10	72	theme	gene	1080:1083	arg1	similarity					1094:1103	97.20 and 97.20 % 16 s rRNA gene sequence similarity	1052:1103	97.20 and 97.20 % 16 s rRNA gene sequence similarity	1052:1103	The closest taxonomically described relatives were Serinibacter salmoneus Kis4-28T and Serinibacter tropicus PS-14-7T, with 97.20 and 97.20 % 16 s rRNA gene sequence similarity, respectively.
31730034	2	73	theme	thawing	150:156	arg1	wedge					170:174	a thawing ancient ice wedge	148:174	a thawing ancient ice wedge at Mammoth Mountain (Eastern Siberia, Russia)	148:220	A novel actinobacterium, strain K3-2T, was isolated in pure culture from a thawing ancient ice wedge at Mammoth Mountain (Eastern Siberia, Russia).
31730034	9	74	theme	gene	851:854	arg1	analysis					856:863	16S rRNA gene analysis	842:863	16S rRNA gene analysis	842:863	16S rRNA gene analysis indicated that strain K3-2T belongs to the genus Serinibacter.
31730034	9	75	theme	rRNA	846:849	arg1	analysis					856:863	16S rRNA gene analysis	842:863	16S rRNA gene analysis	842:863	16S rRNA gene analysis indicated that strain K3-2T belongs to the genus Serinibacter.
31730034	10	76	theme	rRNA	1075:1078	arg1	similarity					1094:1103	97.20 and 97.20 % 16 s rRNA gene sequence similarity	1052:1103	97.20 and 97.20 % 16 s rRNA gene sequence similarity	1052:1103	The closest taxonomically described relatives were Serinibacter salmoneus Kis4-28T and Serinibacter tropicus PS-14-7T, with 97.20 and 97.20 % 16 s rRNA gene sequence similarity, respectively.
31730034	12	77	theme	tropicus	1341:1348	arg1	=PS-14-7T					1365:1373	=PS-14-7T	1365:1373	=PS-14-7T	1365:1373	DNA-DNA relatedness values between strain K3-2T and S. salmoneus DSM 21801T (=Kis4-28T) and S. tropicus VKPM Ac 2044T (=PS-14-7T) were 41 and 47 %.
31730034	12	77	theme	tropicus	1341:1348	arg1	2044T					1358:1362	S. tropicus VKPM Ac 2044T	1338:1362	S. tropicus VKPM Ac 2044T (=PS-14-7T)	1338:1374	DNA-DNA relatedness values between strain K3-2T and S. salmoneus DSM 21801T (=Kis4-28T) and S. tropicus VKPM Ac 2044T (=PS-14-7T) were 41 and 47 %.
31730034	2	78	theme	Eastern	197:203	arg1	Mountain					187:194	Mountain	187:194	Mountain	187:194	A novel actinobacterium, strain K3-2T, was isolated in pure culture from a thawing ancient ice wedge at Mammoth Mountain (Eastern Siberia, Russia).
31730034	2	78	theme	Eastern	197:203	arg1	Siberia					205:211	Eastern Siberia	197:211	Eastern Siberia	197:211	A novel actinobacterium, strain K3-2T, was isolated in pure culture from a thawing ancient ice wedge at Mammoth Mountain (Eastern Siberia, Russia).
31730034	3	79	contain	had	277:279	arg1	cells					271:275	cells	271:275	cells	271:275	Colonies of strain K3-2T were yellowish orange; cells had the fine structure typical of Gram-positive bacteria, were non-motile short rods and were non-spore-forming.
31730034	3	79	contain	had	277:279	arg2	structure					290:298	the fine structure typical	281:306	the fine structure typical of Gram-positive bacteria	281:332	Colonies of strain K3-2T were yellowish orange; cells had the fine structure typical of Gram-positive bacteria, were non-motile short rods and were non-spore-forming.
31730034	3	80	theme	bacteria	325:332	arg1	structure					290:298	the fine structure typical	281:306	the fine structure typical of Gram-positive bacteria	281:332	Colonies of strain K3-2T were yellowish orange; cells had the fine structure typical of Gram-positive bacteria, were non-motile short rods and were non-spore-forming.
31730034	10	81	theme	16 s	1070:1073	arg1	similarity					1094:1103	97.20 and 97.20 % 16 s rRNA gene sequence similarity	1052:1103	97.20 and 97.20 % 16 s rRNA gene sequence similarity	1052:1103	The closest taxonomically described relatives were Serinibacter salmoneus Kis4-28T and Serinibacter tropicus PS-14-7T, with 97.20 and 97.20 % 16 s rRNA gene sequence similarity, respectively.
31769751	5	0	dep	%	552:552	arg1	w/v					555:557	w/v	555:557	w/v	555:557	Strain 16Sb5-5T grew optimally at 37 °C, pH 7.0 and with 0‒2 % (w/v) NaCl.
31769751	4	1	dep	Gram-staining-positive	425:446	arg1	shaped					483:488	shaped	483:488	shaped	483:488	Cells of the isolate were Gram-staining-positive, aerobic, non-motile and short-rod shaped.
31769751	12	2	theme	%	1417:1417	arg1	value					1428:1432	94.8-94.9 % and GGDC value	1407:1432	94.8-94.9 % and GGDC value of 59.5 % to D. aurantiacus CPCC 204711T	1407:1473	In silico genomic comparisons showed that strain 16Sb5-5T exhibited ANI values of 94.8-94.9 % and GGDC value of 59.5 % to D. aurantiacus CPCC 204711T.
31769751	11	3	dep	D.	1283:1284	arg1	aurantiacus					1286:1296	D. aurantiacus	1283:1296	D. aurantiacus in the phylogenetic trees	1283:1322	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 16Sb5-5T was closely related to Desertihabitans aurantiacus CPCC 204711T (99.8 % similarity) and formed a robust clade with D. aurantiacus in the phylogenetic trees.
31769751	12	4	theme	strain	1367:1372	arg1	16Sb5-5T					1374:1381	strain 16Sb5-5T	1367:1381	strain 16Sb5-5T	1367:1381	In silico genomic comparisons showed that strain 16Sb5-5T exhibited ANI values of 94.8-94.9 % and GGDC value of 59.5 % to D. aurantiacus CPCC 204711T.
31769751	0	5	dep	sp	23:24	arg1	brevis					16:21	Desertihabitans brevis	0:21	Desertihabitans brevis	0:21	Desertihabitans brevis sp.
31769751	12	6	theme	ANI	1393:1395	arg1	values					1397:1402	ANI values	1393:1402	ANI values of 94.8-94.9 % and GGDC value of 59.5 % to D. aurantiacus CPCC 204711T	1393:1473	In silico genomic comparisons showed that strain 16Sb5-5T exhibited ANI values of 94.8-94.9 % and GGDC value of 59.5 % to D. aurantiacus CPCC 204711T.
31769751	6	7	contain	contained	618:626	arg2	alanine					628:634	alanine	628:634	alanine	628:634	The cell-wall peptidoglycan was of the A3γ type and contained alanine, glycine, glutamic acid and ll-diaminopimelic acid (ll-DAP).
31769751	6	7	contain	contained	618:626	arg2	acid					655:658	glutamic acid	646:658	glutamic acid	646:658	The cell-wall peptidoglycan was of the A3γ type and contained alanine, glycine, glutamic acid and ll-diaminopimelic acid (ll-DAP).
31769751	6	7	contain	contained	618:626	arg2	glycine					637:643	glycine	637:643	glycine	637:643	The cell-wall peptidoglycan was of the A3γ type and contained alanine, glycine, glutamic acid and ll-diaminopimelic acid (ll-DAP).
31769751	6	7	contain	contained	618:626	arg1	peptidoglycan					580:592	The cell-wall peptidoglycan	566:592	The cell-wall peptidoglycan	566:592	The cell-wall peptidoglycan was of the A3γ type and contained alanine, glycine, glutamic acid and ll-diaminopimelic acid (ll-DAP).
31769751	6	7	contain	contained	618:626	arg2	acid					682:685	ll-diaminopimelic acid	664:685	ll-diaminopimelic acid	664:685	The cell-wall peptidoglycan was of the A3γ type and contained alanine, glycine, glutamic acid and ll-diaminopimelic acid (ll-DAP).
31769751	6	7	contain	contained	618:626	arg2	ll-DAP					688:693	ll-DAP	688:693	ll-DAP	688:693	The cell-wall peptidoglycan was of the A3γ type and contained alanine, glycine, glutamic acid and ll-diaminopimelic acid (ll-DAP).
31769751	12	8	theme	In	1325:1326	arg1	comparisons					1343:1353	In silico genomic comparisons	1325:1353	In silico genomic comparisons	1325:1353	In silico genomic comparisons showed that strain 16Sb5-5T exhibited ANI values of 94.8-94.9 % and GGDC value of 59.5 % to D. aurantiacus CPCC 204711T.
31769751	12	9	theme	genomic	1335:1341	arg1	comparisons					1343:1353	In silico genomic comparisons	1325:1353	In silico genomic comparisons	1325:1353	In silico genomic comparisons showed that strain 16Sb5-5T exhibited ANI values of 94.8-94.9 % and GGDC value of 59.5 % to D. aurantiacus CPCC 204711T.
31769751	16	10	theme	=KCTC	1819:1823	arg1	16Sb5-5T					1809:1816	16Sb5-5T	1809:1816	16Sb5-5T (=KCTC 49116T=CGMCC 1.16553T)	1809:1846	The type strain is 16Sb5-5T (=KCTC 49116T=CGMCC 1.16553T).
31769751	16	10	theme	=KCTC	1819:1823	arg1	1.16553T					1838:1845	=KCTC 49116T=CGMCC 1.16553T	1819:1845	=KCTC 49116T=CGMCC 1.16553T	1819:1845	The type strain is 16Sb5-5T (=KCTC 49116T=CGMCC 1.16553T).
31769751	4	11	theme	isolate	412:418	arg1	Cells					399:403	Cells	399:403	Cells of the isolate	399:418	Cells of the isolate were Gram-staining-positive, aerobic, non-motile and short-rod shaped.
31769751	14	12	theme	closest	1635:1641	arg1	relative					1656:1663	its closest phylogenetic relative	1631:1663	its closest phylogenetic relative	1631:1663	On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 16Sb5-5T could be distinguishable from its closest phylogenetic relative and represents a novel species of the genus Desertihabitans, for which the name Desertihabitans brevis sp.
31769751	13	13	theme	G+C	1488:1490	arg1	content					1492:1498	The genomic G+C content	1476:1498	The genomic G+C content	1476:1498	The genomic G+C content was 73.3 mol%.
31769751	13	13	theme	G+C	1488:1490	arg1	%					1512:1512	73.3 mol%	1504:1512	73.3 mol%	1504:1512	The genomic G+C content was 73.3 mol%.
31769751	17	14	theme	Desertihabitans	1878:1892	arg1	description					1853:1863	The description	1849:1863	The description of the genus Desertihabitans	1849:1892	The description of the genus Desertihabitans has also been emended.
31769751	10	15	theme	whole-cell	1021:1030	arg1	anteiso-C15 					1049:1060	anteiso-C15 	1049:1060	anteiso-C15 	1049:1060	The major whole-cell fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
31769751	10	15	theme	whole-cell	1021:1030	arg1	acids					1038:1042	The major whole-cell fatty acids	1011:1042	The major whole-cell fatty acids	1011:1042	The major whole-cell fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
31769751	1	16	theme	emended	101:107	arg1	description					109:119	emended description	101:119	emended description of the genus Desertihabitans	101:148	nov., an actinobacterium isolated from sand of the Taklamakan desert, and emended description of the genus Desertihabitans.
31769751	2	17	theme	sand	224:227	arg1	sample					229:234	a sand sample	222:234	a sand sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, China	222:312	A novel actinobacterium, designated strain 16Sb5-5T, was isolated from a sand sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, China.
31769751	1	18	theme	genus	128:132	arg1	Desertihabitans					134:148	the genus Desertihabitans	124:148	the genus Desertihabitans	124:148	nov., an actinobacterium isolated from sand of the Taklamakan desert, and emended description of the genus Desertihabitans.
31769751	8	19	theme	predominant	777:787	arg1	MK-9					805:808	MK-9	805:808	MK-9(H4)	805:812	The predominant menaquinone was MK-9(H4).
31769751	8	19	theme	predominant	777:787	arg1	menaquinone					789:799	The predominant menaquinone	773:799	The predominant menaquinone	773:799	The predominant menaquinone was MK-9(H4).
31769751	6	20	theme	cell-wall	570:578	arg1	peptidoglycan					580:592	The cell-wall peptidoglycan	566:592	The cell-wall peptidoglycan	566:592	The cell-wall peptidoglycan was of the A3γ type and contained alanine, glycine, glutamic acid and ll-diaminopimelic acid (ll-DAP).
31769751	12	21	theme	CPCC	1462:1465	arg1	204711T					1467:1473	D. aurantiacus CPCC 204711T	1447:1473	D. aurantiacus CPCC 204711T	1447:1473	In silico genomic comparisons showed that strain 16Sb5-5T exhibited ANI values of 94.8-94.9 % and GGDC value of 59.5 % to D. aurantiacus CPCC 204711T.
31769751	11	22	theme	99.8 	1233:1237	arg1	similarity					1240:1249	99.8 % similarity	1233:1249	99.8 % similarity	1233:1249	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 16Sb5-5T was closely related to Desertihabitans aurantiacus CPCC 204711T (99.8 % similarity) and formed a robust clade with D. aurantiacus in the phylogenetic trees.
31769751	11	22	theme	99.8 	1233:1237	arg1	204711T					1224:1230	Desertihabitans aurantiacus CPCC 204711T	1191:1230	Desertihabitans aurantiacus CPCC 204711T (99.8 % similarity)	1191:1250	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 16Sb5-5T was closely related to Desertihabitans aurantiacus CPCC 204711T (99.8 % similarity) and formed a robust clade with D. aurantiacus in the phylogenetic trees.
31769751	14	23	theme	chemotaxonomic	1560:1573	arg1	analyses					1575:1582	phylogenetic, phenotypic and chemotaxonomic analyses	1531:1582	phylogenetic, phenotypic and chemotaxonomic analyses	1531:1582	On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 16Sb5-5T could be distinguishable from its closest phylogenetic relative and represents a novel species of the genus Desertihabitans, for which the name Desertihabitans brevis sp.
31769751	2	24	attach	isolated	208:215	arg1	sample					229:234	a sand sample	222:234	a sand sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, China	222:312	A novel actinobacterium, designated strain 16Sb5-5T, was isolated from a sand sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, China.
31769751	2	24	attach	isolated	208:215	arg2	actinobacterium					159:173	A novel actinobacterium	151:173	A novel actinobacterium	151:173	A novel actinobacterium, designated strain 16Sb5-5T, was isolated from a sand sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, China.
31769751	14	25	theme	phenotypic	1545:1554	arg1	analyses					1575:1582	phylogenetic, phenotypic and chemotaxonomic analyses	1531:1582	phylogenetic, phenotypic and chemotaxonomic analyses	1531:1582	On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 16Sb5-5T could be distinguishable from its closest phylogenetic relative and represents a novel species of the genus Desertihabitans, for which the name Desertihabitans brevis sp.
31769751	11	26	theme	gene	1122:1125	arg1	sequences					1127:1135	16S rRNA gene sequences	1113:1135	16S rRNA gene sequences	1113:1135	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 16Sb5-5T was closely related to Desertihabitans aurantiacus CPCC 204711T (99.8 % similarity) and formed a robust clade with D. aurantiacus in the phylogenetic trees.
31769751	0	27	theme	Desertihabitans	0:14	arg1	brevis					16:21	Desertihabitans brevis	0:21	Desertihabitans brevis	0:21	Desertihabitans brevis sp.
31769751	14	28	theme	brevis	1761:1766	arg1	sp					1768:1769	the name Desertihabitans brevis sp	1736:1769	the name Desertihabitans brevis sp	1736:1769	On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 16Sb5-5T could be distinguishable from its closest phylogenetic relative and represents a novel species of the genus Desertihabitans, for which the name Desertihabitans brevis sp.
31769751	5	29	theme	0‒2 	548:551	arg1	%					552:552	%	552:552	%	552:552	Strain 16Sb5-5T grew optimally at 37 °C, pH 7.0 and with 0‒2 % (w/v) NaCl.
31769751	1	30	theme	Taklamakan	78:87	arg1	desert					89:94	the Taklamakan desert	74:94	the Taklamakan desert	74:94	nov., an actinobacterium isolated from sand of the Taklamakan desert, and emended description of the genus Desertihabitans.
31769751	14	31	theme	name	1740:1743	arg1	sp					1768:1769	the name Desertihabitans brevis sp	1736:1769	the name Desertihabitans brevis sp	1736:1769	On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 16Sb5-5T could be distinguishable from its closest phylogenetic relative and represents a novel species of the genus Desertihabitans, for which the name Desertihabitans brevis sp.
31769751	11	32	theme	strain	1152:1157	arg1	16Sb5-5T					1159:1166	strain 16Sb5-5T	1152:1166	strain 16Sb5-5T	1152:1166	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 16Sb5-5T was closely related to Desertihabitans aurantiacus CPCC 204711T (99.8 % similarity) and formed a robust clade with D. aurantiacus in the phylogenetic trees.
31769751	3	33	theme	taxonomic	379:387	arg1	position					389:396	its taxonomic position	375:396	its taxonomic position	375:396	The strain was examined by a polyphasic approach to clarify its taxonomic position.
31769751	12	34	theme	aurantiacus	1450:1460	arg1	204711T					1467:1473	D. aurantiacus CPCC 204711T	1447:1473	D. aurantiacus CPCC 204711T	1447:1473	In silico genomic comparisons showed that strain 16Sb5-5T exhibited ANI values of 94.8-94.9 % and GGDC value of 59.5 % to D. aurantiacus CPCC 204711T.
31769751	11	35	theme	Phylogenetic	1082:1093	arg1	analysis					1095:1102	Phylogenetic analysis	1082:1102	Phylogenetic analysis based on 16S rRNA gene sequences	1082:1135	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 16Sb5-5T was closely related to Desertihabitans aurantiacus CPCC 204711T (99.8 % similarity) and formed a robust clade with D. aurantiacus in the phylogenetic trees.
31769751	12	36	theme	value	1428:1432	arg1	values					1397:1402	ANI values	1393:1402	ANI values of 94.8-94.9 % and GGDC value of 59.5 % to D. aurantiacus CPCC 204711T	1393:1473	In silico genomic comparisons showed that strain 16Sb5-5T exhibited ANI values of 94.8-94.9 % and GGDC value of 59.5 % to D. aurantiacus CPCC 204711T.
31769751	9	37	theme	polar	819:823	arg1	profile					831:837	The polar lipid profile	815:837	The polar lipid profile	815:837	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified phospholipid, three unidentified glycolipids and three unidentified lipids.
31769751	12	38	dep	In	1325:1326	arg1	silico					1328:1333	silico	1328:1333	silico	1328:1333	In silico genomic comparisons showed that strain 16Sb5-5T exhibited ANI values of 94.8-94.9 % and GGDC value of 59.5 % to D. aurantiacus CPCC 204711T.
31769751	14	39	theme	genus	1703:1707	arg1	Desertihabitans					1709:1723	the genus Desertihabitans	1699:1723	the genus Desertihabitans	1699:1723	On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 16Sb5-5T could be distinguishable from its closest phylogenetic relative and represents a novel species of the genus Desertihabitans, for which the name Desertihabitans brevis sp.
31769751	11	40	theme	16S	1113:1115	arg1	sequences					1127:1135	16S rRNA gene sequences	1113:1135	16S rRNA gene sequences	1113:1135	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 16Sb5-5T was closely related to Desertihabitans aurantiacus CPCC 204711T (99.8 % similarity) and formed a robust clade with D. aurantiacus in the phylogenetic trees.
31769751	6	41	theme	ll-diaminopimelic	664:680	arg1	acid					682:685	ll-diaminopimelic acid	664:685	ll-diaminopimelic acid	664:685	The cell-wall peptidoglycan was of the A3γ type and contained alanine, glycine, glutamic acid and ll-diaminopimelic acid (ll-DAP).
31769751	12	42	theme	94.8-94.9 	1407:1416	arg1	%					1417:1417	%	1417:1417	%	1417:1417	In silico genomic comparisons showed that strain 16Sb5-5T exhibited ANI values of 94.8-94.9 % and GGDC value of 59.5 % to D. aurantiacus CPCC 204711T.
31769751	14	43	theme	novel	1682:1686	arg1	species					1688:1694	a novel species	1680:1694	a novel species	1680:1694	On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 16Sb5-5T could be distinguishable from its closest phylogenetic relative and represents a novel species of the genus Desertihabitans, for which the name Desertihabitans brevis sp.
31769751	12	44	theme	 and	1418:1421	arg1	value					1428:1432	94.8-94.9 % and GGDC value	1407:1432	94.8-94.9 % and GGDC value of 59.5 % to D. aurantiacus CPCC 204711T	1407:1473	In silico genomic comparisons showed that strain 16Sb5-5T exhibited ANI values of 94.8-94.9 % and GGDC value of 59.5 % to D. aurantiacus CPCC 204711T.
31769751	16	45	theme	type	1794:1797	arg1	16Sb5-5T					1809:1816	16Sb5-5T	1809:1816	16Sb5-5T (=KCTC 49116T=CGMCC 1.16553T)	1809:1846	The type strain is 16Sb5-5T (=KCTC 49116T=CGMCC 1.16553T).
31769751	16	45	theme	type	1794:1797	arg1	strain					1799:1804	The type strain	1790:1804	The type strain	1790:1804	The type strain is 16Sb5-5T (=KCTC 49116T=CGMCC 1.16553T).
31769751	14	46	dep	analyses	1575:1582	arg1	basis					1522:1526	basis	1522:1526	basis	1522:1526	On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 16Sb5-5T could be distinguishable from its closest phylogenetic relative and represents a novel species of the genus Desertihabitans, for which the name Desertihabitans brevis sp.
31769751	14	46	dep	analyses	1575:1582	arg1	the					1518:1520	the	1518:1520	the	1518:1520	On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 16Sb5-5T could be distinguishable from its closest phylogenetic relative and represents a novel species of the genus Desertihabitans, for which the name Desertihabitans brevis sp.
31769751	2	47	theme	Taklamakan	253:262	arg1	desert					264:269	the Taklamakan desert	249:269	the Taklamakan desert	249:269	A novel actinobacterium, designated strain 16Sb5-5T, was isolated from a sand sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, China.
31769751	13	48	theme	73.3 mol	1504:1511	arg1	content					1492:1498	The genomic G+C content	1476:1498	The genomic G+C content	1476:1498	The genomic G+C content was 73.3 mol%.
31769751	13	48	theme	73.3 mol	1504:1511	arg1	%					1512:1512	73.3 mol%	1504:1512	73.3 mol%	1504:1512	The genomic G+C content was 73.3 mol%.
31769751	7	49	located	detected	732:739	arg1	hydrolysates					759:770	the whole-cell hydrolysates	744:770	the whole-cell hydrolysates	744:770	Ribose, arabinose and glucose were detected in the whole-cell hydrolysates.
31769751	7	49	located	detected	732:739	arg2	Ribose					697:702	Ribose	697:702	Ribose	697:702	Ribose, arabinose and glucose were detected in the whole-cell hydrolysates.
31769751	7	49	located	detected	732:739	arg2	glucose					719:725	glucose	719:725	glucose	719:725	Ribose, arabinose and glucose were detected in the whole-cell hydrolysates.
31769751	7	49	located	detected	732:739	arg2	arabinose					705:713	arabinose	705:713	arabinose	705:713	Ribose, arabinose and glucose were detected in the whole-cell hydrolysates.
31769751	9	50	theme	unidentified	922:933	arg1	phospholipid					935:946	an unidentified phospholipid	919:946	an unidentified phospholipid	919:946	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified phospholipid, three unidentified glycolipids and three unidentified lipids.
31769751	1	51	attach	isolated	52:59	arg2	actinobacterium					36:50	an actinobacterium	33:50	an actinobacterium isolated from sand of the Taklamakan desert	33:94	nov., an actinobacterium isolated from sand of the Taklamakan desert, and emended description of the genus Desertihabitans.
31769751	1	51	attach	isolated	52:59	arg1	sand					66:69	sand	66:69	sand of the Taklamakan desert	66:94	nov., an actinobacterium isolated from sand of the Taklamakan desert, and emended description of the genus Desertihabitans.
31769751	16	52	theme	49116T=CGMCC	1825:1836	arg1	16Sb5-5T					1809:1816	16Sb5-5T	1809:1816	16Sb5-5T (=KCTC 49116T=CGMCC 1.16553T)	1809:1846	The type strain is 16Sb5-5T (=KCTC 49116T=CGMCC 1.16553T).
31769751	16	52	theme	49116T=CGMCC	1825:1836	arg1	1.16553T					1838:1845	=KCTC 49116T=CGMCC 1.16553T	1819:1845	=KCTC 49116T=CGMCC 1.16553T	1819:1845	The type strain is 16Sb5-5T (=KCTC 49116T=CGMCC 1.16553T).
31769751	11	53	theme	phylogenetic	1305:1316	arg1	trees					1318:1322	the phylogenetic trees	1301:1322	the phylogenetic trees	1301:1322	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 16Sb5-5T was closely related to Desertihabitans aurantiacus CPCC 204711T (99.8 % similarity) and formed a robust clade with D. aurantiacus in the phylogenetic trees.
31769751	14	54	from	relative	1656:1663	arg1	distinguishable					1610:1624	distinguishable	1610:1624	distinguishable	1610:1624	On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 16Sb5-5T could be distinguishable from its closest phylogenetic relative and represents a novel species of the genus Desertihabitans, for which the name Desertihabitans brevis sp.
31769751	14	55	theme	phylogenetic	1643:1654	arg1	relative					1656:1663	its closest phylogenetic relative	1631:1663	its closest phylogenetic relative	1631:1663	On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 16Sb5-5T could be distinguishable from its closest phylogenetic relative and represents a novel species of the genus Desertihabitans, for which the name Desertihabitans brevis sp.
31769751	13	56	theme	genomic	1480:1486	arg1	content					1492:1498	The genomic G+C content	1476:1498	The genomic G+C content	1476:1498	The genomic G+C content was 73.3 mol%.
31769751	13	56	theme	genomic	1480:1486	arg1	%					1512:1512	73.3 mol%	1504:1512	73.3 mol%	1504:1512	The genomic G+C content was 73.3 mol%.
31769751	7	57	theme	whole-cell	748:757	arg1	hydrolysates					759:770	the whole-cell hydrolysates	744:770	the whole-cell hydrolysates	744:770	Ribose, arabinose and glucose were detected in the whole-cell hydrolysates.
31769751	17	58	theme	genus	1872:1876	arg1	Desertihabitans					1878:1892	the genus Desertihabitans	1868:1892	the genus Desertihabitans	1868:1892	The description of the genus Desertihabitans has also been emended.
31769751	14	59	theme	phylogenetic	1531:1542	arg1	analyses					1575:1582	phylogenetic, phenotypic and chemotaxonomic analyses	1531:1582	phylogenetic, phenotypic and chemotaxonomic analyses	1531:1582	On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 16Sb5-5T could be distinguishable from its closest phylogenetic relative and represents a novel species of the genus Desertihabitans, for which the name Desertihabitans brevis sp.
31769751	9	60	theme	unidentified	955:966	arg1	glycolipids					968:978	three unidentified glycolipids	949:978	three unidentified glycolipids	949:978	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified phospholipid, three unidentified glycolipids and three unidentified lipids.
31769751	14	61	theme	analyses	1575:1582	arg1	distinguishable					1610:1624	distinguishable	1610:1624	distinguishable	1610:1624	On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 16Sb5-5T could be distinguishable from its closest phylogenetic relative and represents a novel species of the genus Desertihabitans, for which the name Desertihabitans brevis sp.
31769751	10	62	dep	anteiso-C15 	1049:1060	arg1	 0					1062:1063	 0	1062:1063	 0	1062:1063	The major whole-cell fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
31769751	10	62	dep	anteiso-C15 	1049:1060	arg1	 0					1078:1079	 0	1078:1079	anteiso-C15 : 0 and iso-C15 : 0	1049:1079	The major whole-cell fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
31769751	10	62	dep	anteiso-C15 	1049:1060	arg1	iso-C15 					1069:1076	iso-C15 	1069:1076	iso-C15 	1069:1076	The major whole-cell fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
31769751	6	63	theme	A3γ	605:607	arg1	type					609:612	the A3γ type	601:612	the A3γ type	601:612	The cell-wall peptidoglycan was of the A3γ type and contained alanine, glycine, glutamic acid and ll-diaminopimelic acid (ll-DAP).
31769751	10	64	theme	major	1015:1019	arg1	anteiso-C15 					1049:1060	anteiso-C15 	1049:1060	anteiso-C15 	1049:1060	The major whole-cell fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
31769751	10	64	theme	major	1015:1019	arg1	acids					1038:1042	The major whole-cell fatty acids	1011:1042	The major whole-cell fatty acids	1011:1042	The major whole-cell fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
31769751	9	65	theme	unidentified	990:1001	arg1	lipids					1003:1008	three unidentified lipids	984:1008	three unidentified lipids	984:1008	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified phospholipid, three unidentified glycolipids and three unidentified lipids.
31769751	14	66	theme	strain	1585:1590	arg1	16Sb5-5T					1592:1599	strain 16Sb5-5T	1585:1599	strain 16Sb5-5T	1585:1599	On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 16Sb5-5T could be distinguishable from its closest phylogenetic relative and represents a novel species of the genus Desertihabitans, for which the name Desertihabitans brevis sp.
31769751	9	67	theme	lipid	825:829	arg1	profile					831:837	The polar lipid profile	815:837	The polar lipid profile	815:837	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, an unidentified phospholipid, three unidentified glycolipids and three unidentified lipids.
31769751	1	68	theme	Desertihabitans	134:148	arg1	description					109:119	emended description	101:119	emended description of the genus Desertihabitans	101:148	nov., an actinobacterium isolated from sand of the Taklamakan desert, and emended description of the genus Desertihabitans.
31769751	1	68	theme	Desertihabitans	134:148	arg1	nov.					27:30	nov.	27:30	nov.	27:30	nov., an actinobacterium isolated from sand of the Taklamakan desert, and emended description of the genus Desertihabitans.
31769751	1	68	theme	Desertihabitans	134:148	arg1	actinobacterium					36:50	an actinobacterium	33:50	an actinobacterium isolated from sand of the Taklamakan desert	33:94	nov., an actinobacterium isolated from sand of the Taklamakan desert, and emended description of the genus Desertihabitans.
31769751	11	69	dep	Desertihabitans	1191:1205	arg1	aurantiacus					1207:1217	aurantiacus	1207:1217	aurantiacus	1207:1217	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 16Sb5-5T was closely related to Desertihabitans aurantiacus CPCC 204711T (99.8 % similarity) and formed a robust clade with D. aurantiacus in the phylogenetic trees.
31769751	10	70	theme	fatty	1032:1036	arg1	anteiso-C15 					1049:1060	anteiso-C15 	1049:1060	anteiso-C15 	1049:1060	The major whole-cell fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
31769751	10	70	theme	fatty	1032:1036	arg1	acids					1038:1042	The major whole-cell fatty acids	1011:1042	The major whole-cell fatty acids	1011:1042	The major whole-cell fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
31769751	11	71	theme	%	1238:1238	arg1	similarity					1240:1249	99.8 % similarity	1233:1249	99.8 % similarity	1233:1249	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 16Sb5-5T was closely related to Desertihabitans aurantiacus CPCC 204711T (99.8 % similarity) and formed a robust clade with D. aurantiacus in the phylogenetic trees.
31769751	11	71	theme	%	1238:1238	arg1	204711T					1224:1230	Desertihabitans aurantiacus CPCC 204711T	1191:1230	Desertihabitans aurantiacus CPCC 204711T (99.8 % similarity)	1191:1250	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 16Sb5-5T was closely related to Desertihabitans aurantiacus CPCC 204711T (99.8 % similarity) and formed a robust clade with D. aurantiacus in the phylogenetic trees.
31769751	6	72	theme	glutamic	646:653	arg1	acid					655:658	glutamic acid	646:658	glutamic acid	646:658	The cell-wall peptidoglycan was of the A3γ type and contained alanine, glycine, glutamic acid and ll-diaminopimelic acid (ll-DAP).
31769751	11	73	theme	rRNA	1117:1120	arg1	sequences					1127:1135	16S rRNA gene sequences	1113:1135	16S rRNA gene sequences	1113:1135	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 16Sb5-5T was closely related to Desertihabitans aurantiacus CPCC 204711T (99.8 % similarity) and formed a robust clade with D. aurantiacus in the phylogenetic trees.
31769751	11	74	theme	robust	1265:1270	arg1	clade					1272:1276	a robust clade	1263:1276	a robust clade	1263:1276	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 16Sb5-5T was closely related to Desertihabitans aurantiacus CPCC 204711T (99.8 % similarity) and formed a robust clade with D. aurantiacus in the phylogenetic trees.
31769751	14	75	theme	Desertihabitans	1745:1759	arg1	sp					1768:1769	the name Desertihabitans brevis sp	1736:1769	the name Desertihabitans brevis sp	1736:1769	On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 16Sb5-5T could be distinguishable from its closest phylogenetic relative and represents a novel species of the genus Desertihabitans, for which the name Desertihabitans brevis sp.
31769751	5	76	theme	%	552:552	arg1	NaCl					560:563	0‒2 % (w/v) NaCl	548:563	0‒2 % (w/v) NaCl	548:563	Strain 16Sb5-5T grew optimally at 37 °C, pH 7.0 and with 0‒2 % (w/v) NaCl.
31769751	1	77	theme	desert	89:94	arg1	sand					66:69	sand	66:69	sand of the Taklamakan desert	66:94	nov., an actinobacterium isolated from sand of the Taklamakan desert, and emended description of the genus Desertihabitans.
31769751	3	78	theme	polyphasic	344:353	arg1	approach					355:362	a polyphasic approach	342:362	a polyphasic approach to clarify its taxonomic position	342:396	The strain was examined by a polyphasic approach to clarify its taxonomic position.
31769751	11	79	theme	Desertihabitans	1191:1205	arg1	similarity					1240:1249	99.8 % similarity	1233:1249	99.8 % similarity	1233:1249	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 16Sb5-5T was closely related to Desertihabitans aurantiacus CPCC 204711T (99.8 % similarity) and formed a robust clade with D. aurantiacus in the phylogenetic trees.
31769751	11	79	theme	Desertihabitans	1191:1205	arg1	204711T					1224:1230	Desertihabitans aurantiacus CPCC 204711T	1191:1230	Desertihabitans aurantiacus CPCC 204711T (99.8 % similarity)	1191:1250	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 16Sb5-5T was closely related to Desertihabitans aurantiacus CPCC 204711T (99.8 % similarity) and formed a robust clade with D. aurantiacus in the phylogenetic trees.
31769751	12	80	theme	%	1442:1442	arg1	value					1428:1432	94.8-94.9 % and GGDC value	1407:1432	94.8-94.9 % and GGDC value of 59.5 % to D. aurantiacus CPCC 204711T	1407:1473	In silico genomic comparisons showed that strain 16Sb5-5T exhibited ANI values of 94.8-94.9 % and GGDC value of 59.5 % to D. aurantiacus CPCC 204711T.
31769751	2	81	theme	novel	153:157	arg1	actinobacterium					159:173	A novel actinobacterium	151:173	A novel actinobacterium	151:173	A novel actinobacterium, designated strain 16Sb5-5T, was isolated from a sand sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, China.
31769751	12	82	theme	D.	1447:1448	arg1	204711T					1467:1473	D. aurantiacus CPCC 204711T	1447:1473	D. aurantiacus CPCC 204711T	1447:1473	In silico genomic comparisons showed that strain 16Sb5-5T exhibited ANI values of 94.8-94.9 % and GGDC value of 59.5 % to D. aurantiacus CPCC 204711T.
31769751	14	83	theme	Desertihabitans	1709:1723	arg1	species					1688:1694	a novel species	1680:1694	a novel species	1680:1694	On the basis of phylogenetic, phenotypic and chemotaxonomic analyses, strain 16Sb5-5T could be distinguishable from its closest phylogenetic relative and represents a novel species of the genus Desertihabitans, for which the name Desertihabitans brevis sp.
31769751	12	84	theme	GGDC	1423:1426	arg1	value					1428:1432	94.8-94.9 % and GGDC value	1407:1432	94.8-94.9 % and GGDC value of 59.5 % to D. aurantiacus CPCC 204711T	1407:1473	In silico genomic comparisons showed that strain 16Sb5-5T exhibited ANI values of 94.8-94.9 % and GGDC value of 59.5 % to D. aurantiacus CPCC 204711T.
31769751	11	85	theme	CPCC	1219:1222	arg1	similarity					1240:1249	99.8 % similarity	1233:1249	99.8 % similarity	1233:1249	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 16Sb5-5T was closely related to Desertihabitans aurantiacus CPCC 204711T (99.8 % similarity) and formed a robust clade with D. aurantiacus in the phylogenetic trees.
31769751	11	85	theme	CPCC	1219:1222	arg1	204711T					1224:1230	Desertihabitans aurantiacus CPCC 204711T	1191:1230	Desertihabitans aurantiacus CPCC 204711T (99.8 % similarity)	1191:1250	Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 16Sb5-5T was closely related to Desertihabitans aurantiacus CPCC 204711T (99.8 % similarity) and formed a robust clade with D. aurantiacus in the phylogenetic trees.
31769751	2	86	theme	strain	187:192	arg1	16Sb5-5T					194:201	strain 16Sb5-5T	187:201	strain 16Sb5-5T	187:201	A novel actinobacterium, designated strain 16Sb5-5T, was isolated from a sand sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, China.
31671049	9	0	theme	unidentified	1223:1234	arg1	phospholipids					1236:1248	unidentified phospholipids	1223:1248	unidentified phospholipids	1223:1248	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids and unidentified lipids.
31671049	5	1	dep	Aeromicrobium	779:791	arg1	fastidiosum					793:803	fastidiosum	793:803	fastidiosum	793:803	Phylogenetic analysis based on 16S rRNA gene sequence indicated that M2KJ-4Trepresented a member of the genus Aeromicrobium and shared the highest 16S rRNA gene sequence similarity with Aeromicrobium fastidiosum DSM 10552T (Z78209) (98.95 %).
31671049	11	2	theme	Aeromicrobium	1501:1513	arg1	species					1480:1486	a novel species	1472:1486	a novel species	1472:1486	On the basis of the results from phylogenetic, phenotypic and chemotaxonomic analysis, strain M2KJ-4T represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobium endophyticum sp.
31671049	11	3	from	analysis	1436:1443	arg1	results					1379:1385	the results	1375:1385	the results from phylogenetic, phenotypic and chemotaxonomic analysis	1375:1443	On the basis of the results from phylogenetic, phenotypic and chemotaxonomic analysis, strain M2KJ-4T represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobium endophyticum sp.
31671049	5	4	theme	Aeromicrobium	703:715	arg1	member					683:688	a member	681:688	a member of the genus Aeromicrobium	681:715	Phylogenetic analysis based on 16S rRNA gene sequence indicated that M2KJ-4Trepresented a member of the genus Aeromicrobium and shared the highest 16S rRNA gene sequence similarity with Aeromicrobium fastidiosum DSM 10552T (Z78209) (98.95 %).
31671049	5	5	theme	highest	732:738	arg1	similarity					763:772	the highest 16S rRNA gene sequence similarity	728:772	the highest 16S rRNA gene sequence similarity	728:772	Phylogenetic analysis based on 16S rRNA gene sequence indicated that M2KJ-4Trepresented a member of the genus Aeromicrobium and shared the highest 16S rRNA gene sequence similarity with Aeromicrobium fastidiosum DSM 10552T (Z78209) (98.95 %).
31671049	4	6	theme	Substrate	477:485	arg1	mycelia					487:493	Substrate mycelia	477:493	Substrate mycelia	477:493	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
31671049	7	7	theme	average	886:892	arg1	identity					905:912	The average nucleotide identity	882:912	The average nucleotide identity value and estimated DDH value between M2KJ-4T and the type strain of A. fastidiosum	882:996	The average nucleotide identity value and estimated DDH value between M2KJ-4T and the type strain of A. fastidiosum were 86.1 % and 30.2 %, respectively.
31671049	7	7	theme	average	886:892	arg1	%					1008:1008	86.1 %	1003:1008	86.1 %	1003:1008	The average nucleotide identity value and estimated DDH value between M2KJ-4T and the type strain of A. fastidiosum were 86.1 % and 30.2 %, respectively.
31671049	11	8	dep	results	1379:1385	arg1	basis					1366:1370	basis	1366:1370	basis	1366:1370	On the basis of the results from phylogenetic, phenotypic and chemotaxonomic analysis, strain M2KJ-4T represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobium endophyticum sp.
31671049	11	8	dep	results	1379:1385	arg1	the					1362:1364	the	1362:1364	the	1362:1364	On the basis of the results from phylogenetic, phenotypic and chemotaxonomic analysis, strain M2KJ-4T represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobium endophyticum sp.
31671049	6	9	theme	G+C	844:846	arg1	content					848:854	The DNA G+C content	836:854	The DNA G+C content of M2KJ-4T	836:865	The DNA G+C content of M2KJ-4T was 70.6 mol%.
31671049	6	9	theme	G+C	844:846	arg1	%					879:879	70.6 mol%	871:879	70.6 mol%	871:879	The DNA G+C content of M2KJ-4T was 70.6 mol%.
31671049	7	10	theme	nucleotide	894:903	arg1	identity					905:912	The average nucleotide identity	882:912	The average nucleotide identity value and estimated DDH value between M2KJ-4T and the type strain of A. fastidiosum	882:996	The average nucleotide identity value and estimated DDH value between M2KJ-4T and the type strain of A. fastidiosum were 86.1 % and 30.2 %, respectively.
31671049	7	10	theme	nucleotide	894:903	arg1	%					1008:1008	86.1 %	1003:1008	86.1 %	1003:1008	The average nucleotide identity value and estimated DDH value between M2KJ-4T and the type strain of A. fastidiosum were 86.1 % and 30.2 %, respectively.
31671049	1	11	theme	endophytic	45:54	arg1	actinobacterium					56:70	a novel endophytic actinobacterium	37:70	a novel endophytic actinobacterium isolated from bark of Melia azedaeach L	37:110	nov., a novel endophytic actinobacterium isolated from bark of Melia azedaeach L.
31671049	11	12	theme	phylogenetic	1392:1403	arg1	analysis					1436:1443	phylogenetic, phenotypic and chemotaxonomic analysis	1392:1443	phylogenetic, phenotypic and chemotaxonomic analysis	1392:1443	On the basis of the results from phylogenetic, phenotypic and chemotaxonomic analysis, strain M2KJ-4T represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobium endophyticum sp.
31671049	8	13	theme	ll-diaminopimelic	1074:1090	arg1	acid					1092:1095	ll-diaminopimelic acid	1074:1095	ll-diaminopimelic acid	1074:1095	The cell-wall peptidoglycan contained ll-diaminopimelic acid and MK-9(H4) was the predominant menaquinone.
31671049	6	14	theme	DNA	840:842	arg1	content					848:854	The DNA G+C content	836:854	The DNA G+C content of M2KJ-4T	836:865	The DNA G+C content of M2KJ-4T was 70.6 mol%.
31671049	6	14	theme	DNA	840:842	arg1	%					879:879	70.6 mol%	871:879	70.6 mol%	871:879	The DNA G+C content of M2KJ-4T was 70.6 mol%.
31671049	5	15	theme	16S	624:626	arg1	sequence					638:645	16S rRNA gene sequence	624:645	16S rRNA gene sequence	624:645	Phylogenetic analysis based on 16S rRNA gene sequence indicated that M2KJ-4Trepresented a member of the genus Aeromicrobium and shared the highest 16S rRNA gene sequence similarity with Aeromicrobium fastidiosum DSM 10552T (Z78209) (98.95 %).
31671049	2	16	attach	isolated	226:233	arg1	bark					261:264	a surface-sterilized bark	240:264	a surface-sterilized bark of Melia azedaeach L. collected from Xinpu in Guizhou, PR China	240:328	A Gram-staining-positive, aerobic, rod-shaped, non-spore-forming actinobacterium, designated strain M2KJ-4T, was isolated from a surface-sterilized bark of Melia azedaeach L. collected from Xinpu in Guizhou, PR China and characterized using a polyphasic approach to determine its taxonomic position.
31671049	2	16	attach	isolated	226:233	arg2	actinobacterium					178:192	A Gram-staining-positive, aerobic, rod-shaped, non-spore-forming actinobacterium	113:192	A Gram-staining-positive, aerobic, rod-shaped, non-spore-forming actinobacterium	113:192	A Gram-staining-positive, aerobic, rod-shaped, non-spore-forming actinobacterium, designated strain M2KJ-4T, was isolated from a surface-sterilized bark of Melia azedaeach L. collected from Xinpu in Guizhou, PR China and characterized using a polyphasic approach to determine its taxonomic position.
31671049	5	17	theme	genus	697:701	arg1	Aeromicrobium					703:715	the genus Aeromicrobium	693:715	the genus Aeromicrobium	693:715	Phylogenetic analysis based on 16S rRNA gene sequence indicated that M2KJ-4Trepresented a member of the genus Aeromicrobium and shared the highest 16S rRNA gene sequence similarity with Aeromicrobium fastidiosum DSM 10552T (Z78209) (98.95 %).
31671049	10	18	theme	10-methyl	1311:1319	arg1	C16					1330:1332	C16	1330:1332	C16	1330:1332	The major fatty acids were C16 : 0, 10-methyl C18 : 0, C16 : 0 2-OH and C18 : 1ω9c.
31671049	10	18	theme	10-methyl	1311:1319	arg1	C18					1321:1323	10-methyl C18	1311:1323	10-methyl C18	1311:1323	The major fatty acids were C16 : 0, 10-methyl C18 : 0, C16 : 0 2-OH and C18 : 1ω9c.
31671049	2	19	theme	L.	285:286	arg1	bark					261:264	a surface-sterilized bark	240:264	a surface-sterilized bark of Melia azedaeach L. collected from Xinpu in Guizhou, PR China	240:328	A Gram-staining-positive, aerobic, rod-shaped, non-spore-forming actinobacterium, designated strain M2KJ-4T, was isolated from a surface-sterilized bark of Melia azedaeach L. collected from Xinpu in Guizhou, PR China and characterized using a polyphasic approach to determine its taxonomic position.
31671049	10	20	dep	C16	1302:1304	arg1	0					1308:1308	0	1308:1308	0	1308:1308	The major fatty acids were C16 : 0, 10-methyl C18 : 0, C16 : 0 2-OH and C18 : 1ω9c.
31671049	2	21	theme	taxonomic	393:401	arg1	position					403:410	its taxonomic position	389:410	its taxonomic position	389:410	A Gram-staining-positive, aerobic, rod-shaped, non-spore-forming actinobacterium, designated strain M2KJ-4T, was isolated from a surface-sterilized bark of Melia azedaeach L. collected from Xinpu in Guizhou, PR China and characterized using a polyphasic approach to determine its taxonomic position.
31671049	4	22	theme	diffusible	538:547	arg1	pigments					549:556	no diffusible pigments	535:556	no diffusible pigments	535:556	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
31671049	11	23	theme	endophyticum	1549:1560	arg1	sp					1562:1563	the name Aeromicrobium endophyticum sp	1526:1563	the name Aeromicrobium endophyticum sp	1526:1563	On the basis of the results from phylogenetic, phenotypic and chemotaxonomic analysis, strain M2KJ-4T represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobium endophyticum sp.
31671049	8	24	theme	predominant	1118:1128	arg1	peptidoglycan					1050:1062	The cell-wall peptidoglycan	1036:1062	The cell-wall peptidoglycan contained ll-diaminopimelic acid and MK-9(H4)	1036:1108	The cell-wall peptidoglycan contained ll-diaminopimelic acid and MK-9(H4) was the predominant menaquinone.
31671049	8	24	theme	predominant	1118:1128	arg1	menaquinone					1130:1140	the predominant menaquinone	1114:1140	the predominant menaquinone	1114:1140	The cell-wall peptidoglycan contained ll-diaminopimelic acid and MK-9(H4) was the predominant menaquinone.
31671049	3	25	theme	%	443:443	arg1	NaCl					451:454	1 % (w/v) NaCl	441:454	1 % (w/v) NaCl	441:454	M2KJ-4T grew optimally with 1 % (w/v) NaCl at 25 °C and pH 8.0.
31671049	0	26	theme	endophyticum	14:25	arg1	sp					27:28	Aeromicrobium endophyticum sp	0:28	Aeromicrobium endophyticum sp.	0:29	Aeromicrobium endophyticum sp.
31671049	7	27	theme	86.1	1003:1006	arg1	identity					905:912	The average nucleotide identity	882:912	The average nucleotide identity value and estimated DDH value between M2KJ-4T and the type strain of A. fastidiosum	882:996	The average nucleotide identity value and estimated DDH value between M2KJ-4T and the type strain of A. fastidiosum were 86.1 % and 30.2 %, respectively.
31671049	7	27	theme	86.1	1003:1006	arg1	%					1008:1008	86.1 %	1003:1008	86.1 %	1003:1008	The average nucleotide identity value and estimated DDH value between M2KJ-4T and the type strain of A. fastidiosum were 86.1 % and 30.2 %, respectively.
31671049	8	28	theme	cell-wall	1040:1048	arg1	peptidoglycan					1050:1062	The cell-wall peptidoglycan	1036:1062	The cell-wall peptidoglycan contained ll-diaminopimelic acid and MK-9(H4)	1036:1108	The cell-wall peptidoglycan contained ll-diaminopimelic acid and MK-9(H4) was the predominant menaquinone.
31671049	8	28	theme	cell-wall	1040:1048	arg1	menaquinone					1130:1140	the predominant menaquinone	1114:1140	the predominant menaquinone	1114:1140	The cell-wall peptidoglycan contained ll-diaminopimelic acid and MK-9(H4) was the predominant menaquinone.
31671049	2	29	dep	China	324:328	arg1	PR					321:322	Guizhou, PR China	312:328	PR	321:322	A Gram-staining-positive, aerobic, rod-shaped, non-spore-forming actinobacterium, designated strain M2KJ-4T, was isolated from a surface-sterilized bark of Melia azedaeach L. collected from Xinpu in Guizhou, PR China and characterized using a polyphasic approach to determine its taxonomic position.
31671049	10	30	dep	0	1308:1308	arg1	C16					1330:1332	C16	1330:1332	C16	1330:1332	The major fatty acids were C16 : 0, 10-methyl C18 : 0, C16 : 0 2-OH and C18 : 1ω9c.
31671049	10	30	dep	0	1308:1308	arg1	C18					1321:1323	10-methyl C18	1311:1323	10-methyl C18	1311:1323	The major fatty acids were C16 : 0, 10-methyl C18 : 0, C16 : 0 2-OH and C18 : 1ω9c.
31671049	0	31	theme	Aeromicrobium	0:12	arg1	sp					27:28	Aeromicrobium endophyticum sp	0:28	Aeromicrobium endophyticum sp.	0:29	Aeromicrobium endophyticum sp.
31671049	5	32	theme	Phylogenetic	593:604	arg1	analysis					606:613	Phylogenetic analysis	593:613	Phylogenetic analysis based on 16S rRNA gene sequence	593:645	Phylogenetic analysis based on 16S rRNA gene sequence indicated that M2KJ-4Trepresented a member of the genus Aeromicrobium and shared the highest 16S rRNA gene sequence similarity with Aeromicrobium fastidiosum DSM 10552T (Z78209) (98.95 %).
31671049	11	33	theme	strain	1446:1451	arg1	M2KJ-4T					1453:1459	strain M2KJ-4T	1446:1459	strain M2KJ-4T	1446:1459	On the basis of the results from phylogenetic, phenotypic and chemotaxonomic analysis, strain M2KJ-4T represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobium endophyticum sp.
31671049	2	34	theme	Guizhou	312:318	arg1	China					324:328	Guizhou, PR China	312:328	China	324:328	A Gram-staining-positive, aerobic, rod-shaped, non-spore-forming actinobacterium, designated strain M2KJ-4T, was isolated from a surface-sterilized bark of Melia azedaeach L. collected from Xinpu in Guizhou, PR China and characterized using a polyphasic approach to determine its taxonomic position.
31671049	7	35	dep	identity	905:912	arg1	value					938:942	estimated DDH value	924:942	estimated DDH value	924:942	The average nucleotide identity value and estimated DDH value between M2KJ-4T and the type strain of A. fastidiosum were 86.1 % and 30.2 %, respectively.
31671049	7	35	dep	identity	905:912	arg1	value					914:918	value	914:918	value	914:918	The average nucleotide identity value and estimated DDH value between M2KJ-4T and the type strain of A. fastidiosum were 86.1 % and 30.2 %, respectively.
31671049	5	36	theme	Aeromicrobium	779:791	arg1	Z78209					817:822	Z78209	817:822	Z78209	817:822	Phylogenetic analysis based on 16S rRNA gene sequence indicated that M2KJ-4Trepresented a member of the genus Aeromicrobium and shared the highest 16S rRNA gene sequence similarity with Aeromicrobium fastidiosum DSM 10552T (Z78209) (98.95 %).
31671049	5	36	theme	Aeromicrobium	779:791	arg1	10552T					809:814	Aeromicrobium fastidiosum DSM 10552T	779:814	Aeromicrobium fastidiosum DSM 10552T (Z78209) (98.95 %)	779:833	Phylogenetic analysis based on 16S rRNA gene sequence indicated that M2KJ-4Trepresented a member of the genus Aeromicrobium and shared the highest 16S rRNA gene sequence similarity with Aeromicrobium fastidiosum DSM 10552T (Z78209) (98.95 %).
31671049	5	36	theme	Aeromicrobium	779:791	arg1	%					832:832	98.95 %	826:832	98.95 %	826:832	Phylogenetic analysis based on 16S rRNA gene sequence indicated that M2KJ-4Trepresented a member of the genus Aeromicrobium and shared the highest 16S rRNA gene sequence similarity with Aeromicrobium fastidiosum DSM 10552T (Z78209) (98.95 %).
31671049	7	37	theme	fastidiosum	986:996	arg1	strain					973:978	the type strain	964:978	the type strain of A. fastidiosum	964:996	The average nucleotide identity value and estimated DDH value between M2KJ-4T and the type strain of A. fastidiosum were 86.1 % and 30.2 %, respectively.
31671049	7	37	theme	fastidiosum	986:996	arg1	M2KJ-4T					952:958	M2KJ-4T	952:958	M2KJ-4T	952:958	The average nucleotide identity value and estimated DDH value between M2KJ-4T and the type strain of A. fastidiosum were 86.1 % and 30.2 %, respectively.
31671049	11	38	theme	chemotaxonomic	1421:1434	arg1	analysis					1436:1443	phylogenetic, phenotypic and chemotaxonomic analysis	1392:1443	phylogenetic, phenotypic and chemotaxonomic analysis	1392:1443	On the basis of the results from phylogenetic, phenotypic and chemotaxonomic analysis, strain M2KJ-4T represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobium endophyticum sp.
31671049	1	39	theme	Melia	94:98	arg1	L					110:110	Melia azedaeach L	94:110	Melia azedaeach L	94:110	nov., a novel endophytic actinobacterium isolated from bark of Melia azedaeach L.
31671049	5	40	theme	DSM	805:807	arg1	Z78209					817:822	Z78209	817:822	Z78209	817:822	Phylogenetic analysis based on 16S rRNA gene sequence indicated that M2KJ-4Trepresented a member of the genus Aeromicrobium and shared the highest 16S rRNA gene sequence similarity with Aeromicrobium fastidiosum DSM 10552T (Z78209) (98.95 %).
31671049	5	40	theme	DSM	805:807	arg1	10552T					809:814	Aeromicrobium fastidiosum DSM 10552T	779:814	Aeromicrobium fastidiosum DSM 10552T (Z78209) (98.95 %)	779:833	Phylogenetic analysis based on 16S rRNA gene sequence indicated that M2KJ-4Trepresented a member of the genus Aeromicrobium and shared the highest 16S rRNA gene sequence similarity with Aeromicrobium fastidiosum DSM 10552T (Z78209) (98.95 %).
31671049	5	40	theme	DSM	805:807	arg1	%					832:832	98.95 %	826:832	98.95 %	826:832	Phylogenetic analysis based on 16S rRNA gene sequence indicated that M2KJ-4Trepresented a member of the genus Aeromicrobium and shared the highest 16S rRNA gene sequence similarity with Aeromicrobium fastidiosum DSM 10552T (Z78209) (98.95 %).
31671049	13	41	theme	type	1588:1591	arg1	strain					1593:1598	The type strain	1584:1598	The type strain	1584:1598	The type strain is M2KJ-4T (=KCTC 49174T=CGMCC 1.13666T).
31671049	13	41	theme	type	1588:1591	arg1	M2KJ-4T					1603:1609	M2KJ-4T	1603:1609	M2KJ-4T (=KCTC 49174T=CGMCC 1.13666T)	1603:1639	The type strain is M2KJ-4T (=KCTC 49174T=CGMCC 1.13666T).
31671049	11	42	theme	phenotypic	1406:1415	arg1	analysis					1436:1443	phylogenetic, phenotypic and chemotaxonomic analysis	1392:1443	phylogenetic, phenotypic and chemotaxonomic analysis	1392:1443	On the basis of the results from phylogenetic, phenotypic and chemotaxonomic analysis, strain M2KJ-4T represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobium endophyticum sp.
31671049	10	43	theme	fatty	1285:1289	arg1	acids					1291:1295	The major fatty acids	1275:1295	The major fatty acids	1275:1295	The major fatty acids were C16 : 0, 10-methyl C18 : 0, C16 : 0 2-OH and C18 : 1ω9c.
31671049	10	43	theme	fatty	1285:1289	arg1	C16					1302:1304	C16	1302:1304	C16 : 0, 10-methyl C18 : 0, C16 : 0 2-OH and C18 : 1ω9c	1302:1356	The major fatty acids were C16 : 0, 10-methyl C18 : 0, C16 : 0 2-OH and C18 : 1ω9c.
31671049	1	44	theme	azedaeach	100:108	arg1	L					110:110	Melia azedaeach L	94:110	Melia azedaeach L	94:110	nov., a novel endophytic actinobacterium isolated from bark of Melia azedaeach L.
31671049	5	45	theme	16S	740:742	arg1	similarity					763:772	the highest 16S rRNA gene sequence similarity	728:772	the highest 16S rRNA gene sequence similarity	728:772	Phylogenetic analysis based on 16S rRNA gene sequence indicated that M2KJ-4Trepresented a member of the genus Aeromicrobium and shared the highest 16S rRNA gene sequence similarity with Aeromicrobium fastidiosum DSM 10552T (Z78209) (98.95 %).
31671049	1	46	attach	isolated	72:79	arg1	bark					86:89	bark	86:89	bark of Melia azedaeach L	86:110	nov., a novel endophytic actinobacterium isolated from bark of Melia azedaeach L.
31671049	1	46	attach	isolated	72:79	arg2	actinobacterium					56:70	a novel endophytic actinobacterium	37:70	a novel endophytic actinobacterium isolated from bark of Melia azedaeach L	37:110	nov., a novel endophytic actinobacterium isolated from bark of Melia azedaeach L.
31671049	9	47	theme	predominant	1147:1157	arg1	diphosphatidylglycerol					1177:1198	diphosphatidylglycerol	1177:1198	diphosphatidylglycerol	1177:1198	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids and unidentified lipids.
31671049	9	47	theme	predominant	1147:1157	arg1	lipids					1165:1170	The predominant polar lipids	1143:1170	The predominant polar lipids	1143:1170	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids and unidentified lipids.
31671049	3	48	theme	1	441:441	arg1	%					443:443	%	443:443	%	443:443	M2KJ-4T grew optimally with 1 % (w/v) NaCl at 25 °C and pH 8.0.
31671049	13	49	theme	=KCTC	1612:1616	arg1	1.13666T					1631:1638	=KCTC 49174T=CGMCC 1.13666T	1612:1638	=KCTC 49174T=CGMCC 1.13666T	1612:1638	The type strain is M2KJ-4T (=KCTC 49174T=CGMCC 1.13666T).
31671049	13	49	theme	=KCTC	1612:1616	arg1	M2KJ-4T					1603:1609	M2KJ-4T	1603:1609	M2KJ-4T (=KCTC 49174T=CGMCC 1.13666T)	1603:1639	The type strain is M2KJ-4T (=KCTC 49174T=CGMCC 1.13666T).
31671049	5	50	theme	rRNA	744:747	arg1	similarity					763:772	the highest 16S rRNA gene sequence similarity	728:772	the highest 16S rRNA gene sequence similarity	728:772	Phylogenetic analysis based on 16S rRNA gene sequence indicated that M2KJ-4Trepresented a member of the genus Aeromicrobium and shared the highest 16S rRNA gene sequence similarity with Aeromicrobium fastidiosum DSM 10552T (Z78209) (98.95 %).
31671049	9	51	theme	unidentified	1254:1265	arg1	lipids					1267:1272	unidentified lipids	1254:1272	unidentified lipids	1254:1272	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids and unidentified lipids.
31671049	4	52	located	observed	563:570	arg1	media					579:583	the media	575:583	the media tested	575:590	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
31671049	4	52	located	observed	563:570	arg2	pigments					549:556	no diffusible pigments	535:556	no diffusible pigments	535:556	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
31671049	4	53	theme	aerial	499:504	arg1	mycelia					506:512	aerial mycelia	499:512	aerial mycelia	499:512	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
31671049	5	54	theme	gene	749:752	arg1	similarity					763:772	the highest 16S rRNA gene sequence similarity	728:772	the highest 16S rRNA gene sequence similarity	728:772	Phylogenetic analysis based on 16S rRNA gene sequence indicated that M2KJ-4Trepresented a member of the genus Aeromicrobium and shared the highest 16S rRNA gene sequence similarity with Aeromicrobium fastidiosum DSM 10552T (Z78209) (98.95 %).
31671049	6	55	theme	mol	876:878	arg1	content					848:854	The DNA G+C content	836:854	The DNA G+C content of M2KJ-4T	836:865	The DNA G+C content of M2KJ-4T was 70.6 mol%.
31671049	6	55	theme	mol	876:878	arg1	%					879:879	70.6 mol%	871:879	70.6 mol%	871:879	The DNA G+C content of M2KJ-4T was 70.6 mol%.
31671049	5	56	theme	rRNA	628:631	arg1	sequence					638:645	16S rRNA gene sequence	624:645	16S rRNA gene sequence	624:645	Phylogenetic analysis based on 16S rRNA gene sequence indicated that M2KJ-4Trepresented a member of the genus Aeromicrobium and shared the highest 16S rRNA gene sequence similarity with Aeromicrobium fastidiosum DSM 10552T (Z78209) (98.95 %).
31671049	1	57	theme	L	110:110	arg1	bark					86:89	bark	86:89	bark of Melia azedaeach L	86:110	nov., a novel endophytic actinobacterium isolated from bark of Melia azedaeach L.
31671049	5	58	theme	sequence	754:761	arg1	similarity					763:772	the highest 16S rRNA gene sequence similarity	728:772	the highest 16S rRNA gene sequence similarity	728:772	Phylogenetic analysis based on 16S rRNA gene sequence indicated that M2KJ-4Trepresented a member of the genus Aeromicrobium and shared the highest 16S rRNA gene sequence similarity with Aeromicrobium fastidiosum DSM 10552T (Z78209) (98.95 %).
31671049	2	59	dep	Gram-staining-positive	115:136	arg1	aerobic					139:145	aerobic	139:145	aerobic	139:145	A Gram-staining-positive, aerobic, rod-shaped, non-spore-forming actinobacterium, designated strain M2KJ-4T, was isolated from a surface-sterilized bark of Melia azedaeach L. collected from Xinpu in Guizhou, PR China and characterized using a polyphasic approach to determine its taxonomic position.
31671049	2	59	dep	Gram-staining-positive	115:136	arg1	non-spore-forming					160:176	non-spore-forming	160:176	non-spore-forming	160:176	A Gram-staining-positive, aerobic, rod-shaped, non-spore-forming actinobacterium, designated strain M2KJ-4T, was isolated from a surface-sterilized bark of Melia azedaeach L. collected from Xinpu in Guizhou, PR China and characterized using a polyphasic approach to determine its taxonomic position.
31671049	2	59	dep	Gram-staining-positive	115:136	arg1	rod-shaped					148:157	rod-shaped	148:157	rod-shaped	148:157	A Gram-staining-positive, aerobic, rod-shaped, non-spore-forming actinobacterium, designated strain M2KJ-4T, was isolated from a surface-sterilized bark of Melia azedaeach L. collected from Xinpu in Guizhou, PR China and characterized using a polyphasic approach to determine its taxonomic position.
31671049	5	60	theme	gene	633:636	arg1	sequence					638:645	16S rRNA gene sequence	624:645	16S rRNA gene sequence	624:645	Phylogenetic analysis based on 16S rRNA gene sequence indicated that M2KJ-4Trepresented a member of the genus Aeromicrobium and shared the highest 16S rRNA gene sequence similarity with Aeromicrobium fastidiosum DSM 10552T (Z78209) (98.95 %).
31671049	3	61	dep	%	443:443	arg1	w/v					446:448	w/v	446:448	w/v	446:448	M2KJ-4T grew optimally with 1 % (w/v) NaCl at 25 °C and pH 8.0.
31671049	7	62	theme	DDH	934:936	arg1	value					938:942	estimated DDH value	924:942	estimated DDH value	924:942	The average nucleotide identity value and estimated DDH value between M2KJ-4T and the type strain of A. fastidiosum were 86.1 % and 30.2 %, respectively.
31671049	9	63	theme	polar	1159:1163	arg1	diphosphatidylglycerol					1177:1198	diphosphatidylglycerol	1177:1198	diphosphatidylglycerol	1177:1198	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids and unidentified lipids.
31671049	9	63	theme	polar	1159:1163	arg1	lipids					1165:1170	The predominant polar lipids	1143:1170	The predominant polar lipids	1143:1170	The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids and unidentified lipids.
31671049	1	64	theme	novel	39:43	arg1	actinobacterium					56:70	a novel endophytic actinobacterium	37:70	a novel endophytic actinobacterium isolated from bark of Melia azedaeach L	37:110	nov., a novel endophytic actinobacterium isolated from bark of Melia azedaeach L.
31671049	10	65	theme	major	1279:1283	arg1	acids					1291:1295	The major fatty acids	1275:1295	The major fatty acids	1275:1295	The major fatty acids were C16 : 0, 10-methyl C18 : 0, C16 : 0 2-OH and C18 : 1ω9c.
31671049	10	65	theme	major	1279:1283	arg1	C16					1302:1304	C16	1302:1304	C16 : 0, 10-methyl C18 : 0, C16 : 0 2-OH and C18 : 1ω9c	1302:1356	The major fatty acids were C16 : 0, 10-methyl C18 : 0, C16 : 0 2-OH and C18 : 1ω9c.
31671049	11	66	theme	novel	1474:1478	arg1	species					1480:1486	a novel species	1472:1486	a novel species	1472:1486	On the basis of the results from phylogenetic, phenotypic and chemotaxonomic analysis, strain M2KJ-4T represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobium endophyticum sp.
31671049	2	67	theme	strain	206:211	arg1	M2KJ-4T					213:219	strain M2KJ-4T	206:219	strain M2KJ-4T	206:219	A Gram-staining-positive, aerobic, rod-shaped, non-spore-forming actinobacterium, designated strain M2KJ-4T, was isolated from a surface-sterilized bark of Melia azedaeach L. collected from Xinpu in Guizhou, PR China and characterized using a polyphasic approach to determine its taxonomic position.
31671049	1	68	dep	actinobacterium	56:70	arg1	nov.					31:34	nov.	31:34	nov.	31:34	nov., a novel endophytic actinobacterium isolated from bark of Melia azedaeach L.
31671049	11	69	theme	Aeromicrobium	1535:1547	arg1	sp					1562:1563	the name Aeromicrobium endophyticum sp	1526:1563	the name Aeromicrobium endophyticum sp	1526:1563	On the basis of the results from phylogenetic, phenotypic and chemotaxonomic analysis, strain M2KJ-4T represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobium endophyticum sp.
31671049	6	70	theme	M2KJ-4T	859:865	arg1	content					848:854	The DNA G+C content	836:854	The DNA G+C content of M2KJ-4T	836:865	The DNA G+C content of M2KJ-4T was 70.6 mol%.
31671049	6	70	theme	M2KJ-4T	859:865	arg1	%					879:879	70.6 mol%	871:879	70.6 mol%	871:879	The DNA G+C content of M2KJ-4T was 70.6 mol%.
31671049	7	71	theme	type	968:971	arg1	strain					973:978	the type strain	964:978	the type strain of A. fastidiosum	964:996	The average nucleotide identity value and estimated DDH value between M2KJ-4T and the type strain of A. fastidiosum were 86.1 % and 30.2 %, respectively.
31671049	7	72	theme	estimated	924:932	arg1	value					938:942	estimated DDH value	924:942	estimated DDH value	924:942	The average nucleotide identity value and estimated DDH value between M2KJ-4T and the type strain of A. fastidiosum were 86.1 % and 30.2 %, respectively.
31671049	11	73	theme	name	1530:1533	arg1	sp					1562:1563	the name Aeromicrobium endophyticum sp	1526:1563	the name Aeromicrobium endophyticum sp	1526:1563	On the basis of the results from phylogenetic, phenotypic and chemotaxonomic analysis, strain M2KJ-4T represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobium endophyticum sp.
31671049	13	74	theme	49174T=CGMCC	1618:1629	arg1	1.13666T					1631:1638	=KCTC 49174T=CGMCC 1.13666T	1612:1638	=KCTC 49174T=CGMCC 1.13666T	1612:1638	The type strain is M2KJ-4T (=KCTC 49174T=CGMCC 1.13666T).
31671049	13	74	theme	49174T=CGMCC	1618:1629	arg1	M2KJ-4T					1603:1609	M2KJ-4T	1603:1609	M2KJ-4T (=KCTC 49174T=CGMCC 1.13666T)	1603:1639	The type strain is M2KJ-4T (=KCTC 49174T=CGMCC 1.13666T).
31671049	2	75	theme	Gram-staining-positive	115:136	arg1	actinobacterium					178:192	A Gram-staining-positive, aerobic, rod-shaped, non-spore-forming actinobacterium	113:192	A Gram-staining-positive, aerobic, rod-shaped, non-spore-forming actinobacterium	113:192	A Gram-staining-positive, aerobic, rod-shaped, non-spore-forming actinobacterium, designated strain M2KJ-4T, was isolated from a surface-sterilized bark of Melia azedaeach L. collected from Xinpu in Guizhou, PR China and characterized using a polyphasic approach to determine its taxonomic position.
31671049	2	76	theme	surface-sterilized	242:259	arg1	bark					261:264	a surface-sterilized bark	240:264	a surface-sterilized bark of Melia azedaeach L. collected from Xinpu in Guizhou, PR China	240:328	A Gram-staining-positive, aerobic, rod-shaped, non-spore-forming actinobacterium, designated strain M2KJ-4T, was isolated from a surface-sterilized bark of Melia azedaeach L. collected from Xinpu in Guizhou, PR China and characterized using a polyphasic approach to determine its taxonomic position.
31671049	11	77	theme	genus	1495:1499	arg1	Aeromicrobium					1501:1513	the genus Aeromicrobium	1491:1513	the genus Aeromicrobium	1491:1513	On the basis of the results from phylogenetic, phenotypic and chemotaxonomic analysis, strain M2KJ-4T represents a novel species of the genus Aeromicrobium, for which the name Aeromicrobium endophyticum sp.
31671049	2	78	theme	polyphasic	356:365	arg1	approach					367:374	a polyphasic approach	354:374	a polyphasic approach to determine its taxonomic position	354:410	A Gram-staining-positive, aerobic, rod-shaped, non-spore-forming actinobacterium, designated strain M2KJ-4T, was isolated from a surface-sterilized bark of Melia azedaeach L. collected from Xinpu in Guizhou, PR China and characterized using a polyphasic approach to determine its taxonomic position.
31671049	10	79	dep	C18	1321:1323	arg1	C18					1347:1349	C18	1347:1349	C18	1347:1349	The major fatty acids were C16 : 0, 10-methyl C18 : 0, C16 : 0 2-OH and C18 : 1ω9c.
31671049	10	79	dep	C18	1321:1323	arg1	2-OH					1338:1341	0 2-OH	1336:1341	0 2-OH	1336:1341	The major fatty acids were C16 : 0, 10-methyl C18 : 0, C16 : 0 2-OH and C18 : 1ω9c.
31671049	10	79	dep	C18	1321:1323	arg1	0					1327:1327	0	1327:1327	0	1327:1327	The major fatty acids were C16 : 0, 10-methyl C18 : 0, C16 : 0 2-OH and C18 : 1ω9c.
31671049	10	79	dep	C18	1321:1323	arg1	1ω9c					1353:1356	1ω9c	1353:1356	1ω9c	1353:1356	The major fatty acids were C16 : 0, 10-methyl C18 : 0, C16 : 0 2-OH and C18 : 1ω9c.
31535960	8	0	theme	%	1352:1352	arg1	content					1333:1339	a DNA G+C content	1323:1339	a DNA G+C content of 70.4 mol%	1323:1352	The genome length of strain 21Sc5-5T was 4.67 Mb containing 372 contigs and with a DNA G+C content of 70.4 mol%.
31535960	9	1	theme	Nocardioides	1491:1502	arg1	species					1470:1476	a novel species	1462:1476	a novel species	1462:1476	On the basis of data from phylogenetic, phenotypic and chemotaxonomic analyses, strain 21Sc5-5T represents a novel species of the genus Nocardioides, for which the name Nocardioides vastitatis sp.
31535960	6	2	theme	unidentified	1159:1170	arg1	lipids					1172:1177	three unidentified lipids	1153:1177	three unidentified lipids	1153:1177	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, an unidentified phospholipid and three unidentified lipids.
31535960	3	3	theme	%	444:444	arg1	NaCl					452:455	0-3 % (w/v) NaCl	440:455	0-3 % (w/v) NaCl (optimum, 0 %)	440:470	Strain 21Sc5-5T grew at 10-37 °C (optimum, 28-30 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-3 % (w/v) NaCl (optimum, 0 %).
31535960	3	3	theme	%	444:444	arg1	optimum					458:464	optimum	458:464	optimum	458:464	Strain 21Sc5-5T grew at 10-37 °C (optimum, 28-30 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-3 % (w/v) NaCl (optimum, 0 %).
31535960	2	4	theme	PR	276:277	arg1	Region					268:273	Xinjiang Uygur Autonomous Region	242:273	Xinjiang Uygur Autonomous Region	242:273	A Gram-stain-positive, aerobic, non-motile, rod-shaped actinobacterium, designated strain 21Sc5-5T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China and investigated by using a polyphasic approach.
31535960	2	4	theme	PR	276:277	arg1	China					279:283	PR China	276:283	PR China	276:283	A Gram-stain-positive, aerobic, non-motile, rod-shaped actinobacterium, designated strain 21Sc5-5T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China and investigated by using a polyphasic approach.
31535960	9	5	theme	strain	1435:1440	arg1	21Sc5-5T					1442:1449	strain 21Sc5-5T	1435:1449	strain 21Sc5-5T	1435:1449	On the basis of data from phylogenetic, phenotypic and chemotaxonomic analyses, strain 21Sc5-5T represents a novel species of the genus Nocardioides, for which the name Nocardioides vastitatis sp.
31535960	5	6	theme	predominant	1000:1010	arg1	menaquinone					1012:1022	the predominant menaquinone	996:1022	the predominant menaquinone	996:1022	The results of chemotaxonomic analyses showed that the isolate possessed ll-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan and MK-8(H4) as the predominant menaquinone.
31535960	9	7	theme	name	1519:1522	arg1	sp					1548:1549	the name Nocardioides vastitatis sp	1515:1549	the name Nocardioides vastitatis sp	1515:1549	On the basis of data from phylogenetic, phenotypic and chemotaxonomic analyses, strain 21Sc5-5T represents a novel species of the genus Nocardioides, for which the name Nocardioides vastitatis sp.
31535960	2	8	theme	strain	155:160	arg1	21Sc5-5T					162:169	strain 21Sc5-5T	155:169	strain 21Sc5-5T	155:169	A Gram-stain-positive, aerobic, non-motile, rod-shaped actinobacterium, designated strain 21Sc5-5T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China and investigated by using a polyphasic approach.
31535960	3	9	dep	optimum	368:374	arg1	28-30 °C					377:384	28-30 °C	377:384	28-30 °C	377:384	Strain 21Sc5-5T grew at 10-37 °C (optimum, 28-30 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-3 % (w/v) NaCl (optimum, 0 %).
31535960	4	10	theme	Nocardioides	649:660	arg1	THG-S11.7T					670:679	Nocardioides albidus THG-S11.7T	649:679	Nocardioides albidus THG-S11.7T (97.30 %)	649:689	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain 21Sc5-5T formed a distinct lineage within the genus Nocardioides and had the highest similarity to Nocardioides albidus THG-S11.7T (97.30 %), followed by Nocardioides kongjuensis A2-4T (97.22 %), Nocardioides nitrophenolicus NSP 41T (97.15 %) and Nocardioides caeni MN8T (97.15 %).
31535960	4	10	theme	Nocardioides	649:660	arg1	%					688:688	97.30 %	682:688	97.30 %	682:688	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain 21Sc5-5T formed a distinct lineage within the genus Nocardioides and had the highest similarity to Nocardioides albidus THG-S11.7T (97.30 %), followed by Nocardioides kongjuensis A2-4T (97.22 %), Nocardioides nitrophenolicus NSP 41T (97.15 %) and Nocardioides caeni MN8T (97.15 %).
31535960	4	11	theme	Phylogenetic	473:484	arg1	analysis					486:493	Phylogenetic analysis	473:493	Phylogenetic analysis based on 16S rRNA gene sequences	473:526	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain 21Sc5-5T formed a distinct lineage within the genus Nocardioides and had the highest similarity to Nocardioides albidus THG-S11.7T (97.30 %), followed by Nocardioides kongjuensis A2-4T (97.22 %), Nocardioides nitrophenolicus NSP 41T (97.15 %) and Nocardioides caeni MN8T (97.15 %).
31535960	4	12	contain	had	619:621	arg1	21Sc5-5T					550:557	strain 21Sc5-5T	543:557	strain 21Sc5-5T	543:557	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain 21Sc5-5T formed a distinct lineage within the genus Nocardioides and had the highest similarity to Nocardioides albidus THG-S11.7T (97.30 %), followed by Nocardioides kongjuensis A2-4T (97.22 %), Nocardioides nitrophenolicus NSP 41T (97.15 %) and Nocardioides caeni MN8T (97.15 %).
31535960	4	12	contain	had	619:621	arg2	similarity					635:644	the highest similarity	623:644	the highest similarity to Nocardioides albidus THG-S11.7T (97.30 %)	623:689	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain 21Sc5-5T formed a distinct lineage within the genus Nocardioides and had the highest similarity to Nocardioides albidus THG-S11.7T (97.30 %), followed by Nocardioides kongjuensis A2-4T (97.22 %), Nocardioides nitrophenolicus NSP 41T (97.15 %) and Nocardioides caeni MN8T (97.15 %).
31535960	4	13	theme	gene	513:516	arg1	sequences					518:526	16S rRNA gene sequences	504:526	16S rRNA gene sequences	504:526	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain 21Sc5-5T formed a distinct lineage within the genus Nocardioides and had the highest similarity to Nocardioides albidus THG-S11.7T (97.30 %), followed by Nocardioides kongjuensis A2-4T (97.22 %), Nocardioides nitrophenolicus NSP 41T (97.15 %) and Nocardioides caeni MN8T (97.15 %).
31535960	3	14	theme	w/v	447:449	arg1	NaCl					452:455	0-3 % (w/v) NaCl	440:455	0-3 % (w/v) NaCl (optimum, 0 %)	440:470	Strain 21Sc5-5T grew at 10-37 °C (optimum, 28-30 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-3 % (w/v) NaCl (optimum, 0 %).
31535960	3	14	theme	w/v	447:449	arg1	optimum					458:464	optimum	458:464	optimum	458:464	Strain 21Sc5-5T grew at 10-37 °C (optimum, 28-30 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-3 % (w/v) NaCl (optimum, 0 %).
31535960	7	15	theme	 0	1216:1217	arg1	C18 					1233:1236	 0 and 10-methyl C18 	1216:1236	iso-C16 : 0 and 10-methyl C18 : 0	1207:1239	The major fatty acids were iso-C16 : 0 and 10-methyl C18 : 0.
31535960	11	16	theme	33365T=CGMCC	1604:1615	arg1	21Sc5-5T					1589:1596	21Sc5-5T	1589:1596	21Sc5-5T (=JCM 33365T=CGMCC 4.7608T)	1589:1624	The type strain is 21Sc5-5T (=JCM 33365T=CGMCC 4.7608T).
31535960	11	16	theme	33365T=CGMCC	1604:1615	arg1	4.7608T					1617:1623	=JCM 33365T=CGMCC 4.7608T	1599:1623	=JCM 33365T=CGMCC 4.7608T	1599:1623	The type strain is 21Sc5-5T (=JCM 33365T=CGMCC 4.7608T).
31535960	5	17	theme	peptidoglycan	966:978	arg1	acid					923:926	ll-diaminopimelic acid	905:926	ll-diaminopimelic acid	905:926	The results of chemotaxonomic analyses showed that the isolate possessed ll-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan and MK-8(H4) as the predominant menaquinone.
31535960	5	17	theme	peptidoglycan	966:978	arg1	acid					954:957	the diagnostic diamino acid	931:957	the diagnostic diamino acid of the peptidoglycan and MK-8(H4) as the predominant menaquinone	931:1022	The results of chemotaxonomic analyses showed that the isolate possessed ll-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan and MK-8(H4) as the predominant menaquinone.
31535960	5	18	theme	MK-8	984:987	arg1	acid					923:926	ll-diaminopimelic acid	905:926	ll-diaminopimelic acid	905:926	The results of chemotaxonomic analyses showed that the isolate possessed ll-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan and MK-8(H4) as the predominant menaquinone.
31535960	5	18	theme	MK-8	984:987	arg1	acid					954:957	the diagnostic diamino acid	931:957	the diagnostic diamino acid of the peptidoglycan and MK-8(H4) as the predominant menaquinone	931:1022	The results of chemotaxonomic analyses showed that the isolate possessed ll-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan and MK-8(H4) as the predominant menaquinone.
31535960	2	19	theme	Autonomous	257:266	arg1	Region					268:273	Xinjiang Uygur Autonomous Region	242:273	Xinjiang Uygur Autonomous Region	242:273	A Gram-stain-positive, aerobic, non-motile, rod-shaped actinobacterium, designated strain 21Sc5-5T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China and investigated by using a polyphasic approach.
31535960	2	19	theme	Autonomous	257:266	arg1	China					279:283	PR China	276:283	PR China	276:283	A Gram-stain-positive, aerobic, non-motile, rod-shaped actinobacterium, designated strain 21Sc5-5T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China and investigated by using a polyphasic approach.
31535960	8	20	theme	strain	1263:1268	arg1	21Sc5-5T					1270:1277	strain 21Sc5-5T	1263:1277	strain 21Sc5-5T	1263:1277	The genome length of strain 21Sc5-5T was 4.67 Mb containing 372 contigs and with a DNA G+C content of 70.4 mol%.
31535960	4	21	theme	strain	543:548	arg1	21Sc5-5T					550:557	strain 21Sc5-5T	543:557	strain 21Sc5-5T	543:557	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain 21Sc5-5T formed a distinct lineage within the genus Nocardioides and had the highest similarity to Nocardioides albidus THG-S11.7T (97.30 %), followed by Nocardioides kongjuensis A2-4T (97.22 %), Nocardioides nitrophenolicus NSP 41T (97.15 %) and Nocardioides caeni MN8T (97.15 %).
31535960	11	22	theme	type	1574:1577	arg1	21Sc5-5T					1589:1596	21Sc5-5T	1589:1596	21Sc5-5T (=JCM 33365T=CGMCC 4.7608T)	1589:1624	The type strain is 21Sc5-5T (=JCM 33365T=CGMCC 4.7608T).
31535960	11	22	theme	type	1574:1577	arg1	strain					1579:1584	The type strain	1570:1584	The type strain	1570:1584	The type strain is 21Sc5-5T (=JCM 33365T=CGMCC 4.7608T).
31535960	9	23	theme	Nocardioides	1524:1535	arg1	sp					1548:1549	the name Nocardioides vastitatis sp	1515:1549	the name Nocardioides vastitatis sp	1515:1549	On the basis of data from phylogenetic, phenotypic and chemotaxonomic analyses, strain 21Sc5-5T represents a novel species of the genus Nocardioides, for which the name Nocardioides vastitatis sp.
31535960	2	24	dep	Gram-stain-positive	74:92	arg1	rod-shaped					116:125	rod-shaped	116:125	rod-shaped	116:125	A Gram-stain-positive, aerobic, non-motile, rod-shaped actinobacterium, designated strain 21Sc5-5T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China and investigated by using a polyphasic approach.
31535960	2	24	dep	Gram-stain-positive	74:92	arg1	non-motile					104:113	non-motile	104:113	non-motile	104:113	A Gram-stain-positive, aerobic, non-motile, rod-shaped actinobacterium, designated strain 21Sc5-5T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China and investigated by using a polyphasic approach.
31535960	2	24	dep	Gram-stain-positive	74:92	arg1	aerobic					95:101	aerobic	95:101	aerobic	95:101	A Gram-stain-positive, aerobic, non-motile, rod-shaped actinobacterium, designated strain 21Sc5-5T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China and investigated by using a polyphasic approach.
31535960	4	25	theme	highest	627:633	arg1	similarity					635:644	the highest similarity	623:644	the highest similarity to Nocardioides albidus THG-S11.7T (97.30 %)	623:689	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain 21Sc5-5T formed a distinct lineage within the genus Nocardioides and had the highest similarity to Nocardioides albidus THG-S11.7T (97.30 %), followed by Nocardioides kongjuensis A2-4T (97.22 %), Nocardioides nitrophenolicus NSP 41T (97.15 %) and Nocardioides caeni MN8T (97.15 %).
31535960	9	26	dep	data	1371:1374	arg1	the					1358:1360	the	1358:1360	the	1358:1360	On the basis of data from phylogenetic, phenotypic and chemotaxonomic analyses, strain 21Sc5-5T represents a novel species of the genus Nocardioides, for which the name Nocardioides vastitatis sp.
31535960	9	26	dep	data	1371:1374	arg1	basis					1362:1366	basis	1362:1366	basis	1362:1366	On the basis of data from phylogenetic, phenotypic and chemotaxonomic analyses, strain 21Sc5-5T represents a novel species of the genus Nocardioides, for which the name Nocardioides vastitatis sp.
31535960	9	27	theme	chemotaxonomic	1410:1423	arg1	analyses					1425:1432	phylogenetic, phenotypic and chemotaxonomic analyses	1381:1432	phylogenetic, phenotypic and chemotaxonomic analyses	1381:1432	On the basis of data from phylogenetic, phenotypic and chemotaxonomic analyses, strain 21Sc5-5T represents a novel species of the genus Nocardioides, for which the name Nocardioides vastitatis sp.
31535960	6	28	theme	unidentified	1123:1134	arg1	phospholipid					1136:1147	an unidentified phospholipid	1120:1147	an unidentified phospholipid	1120:1147	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, an unidentified phospholipid and three unidentified lipids.
31535960	4	29	theme	albidus	662:668	arg1	THG-S11.7T					670:679	Nocardioides albidus THG-S11.7T	649:679	Nocardioides albidus THG-S11.7T (97.30 %)	649:689	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain 21Sc5-5T formed a distinct lineage within the genus Nocardioides and had the highest similarity to Nocardioides albidus THG-S11.7T (97.30 %), followed by Nocardioides kongjuensis A2-4T (97.22 %), Nocardioides nitrophenolicus NSP 41T (97.15 %) and Nocardioides caeni MN8T (97.15 %).
31535960	4	29	theme	albidus	662:668	arg1	%					688:688	97.30 %	682:688	97.30 %	682:688	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain 21Sc5-5T formed a distinct lineage within the genus Nocardioides and had the highest similarity to Nocardioides albidus THG-S11.7T (97.30 %), followed by Nocardioides kongjuensis A2-4T (97.22 %), Nocardioides nitrophenolicus NSP 41T (97.15 %) and Nocardioides caeni MN8T (97.15 %).
31535960	7	30	theme	10-methyl	1223:1231	arg1	C18 					1233:1236	 0 and 10-methyl C18 	1216:1236	iso-C16 : 0 and 10-methyl C18 : 0	1207:1239	The major fatty acids were iso-C16 : 0 and 10-methyl C18 : 0.
31535960	8	31	theme	21Sc5-5T	1270:1277	arg1	4.67 Mb					1283:1289	4.67 Mb	1283:1289	4.67 Mb containing 372 contigs	1283:1312	The genome length of strain 21Sc5-5T was 4.67 Mb containing 372 contigs and with a DNA G+C content of 70.4 mol%.
31535960	8	31	theme	21Sc5-5T	1270:1277	arg1	length					1253:1258	The genome length	1242:1258	The genome length of strain 21Sc5-5T	1242:1277	The genome length of strain 21Sc5-5T was 4.67 Mb containing 372 contigs and with a DNA G+C content of 70.4 mol%.
31535960	7	32	dep	iso-C16 	1207:1214	arg1	 0					1238:1239	 0	1238:1239	 0	1238:1239	The major fatty acids were iso-C16 : 0 and 10-methyl C18 : 0.
31535960	7	32	dep	iso-C16 	1207:1214	arg1	C18 					1233:1236	 0 and 10-methyl C18 	1216:1236	iso-C16 : 0 and 10-methyl C18 : 0	1207:1239	The major fatty acids were iso-C16 : 0 and 10-methyl C18 : 0.
31535960	8	33	contain	containing	1291:1300	arg2	contigs					1306:1312	372 contigs	1302:1312	372 contigs	1302:1312	The genome length of strain 21Sc5-5T was 4.67 Mb containing 372 contigs and with a DNA G+C content of 70.4 mol%.
31535960	8	33	contain	containing	1291:1300	arg1	4.67 Mb					1283:1289	4.67 Mb	1283:1289	4.67 Mb containing 372 contigs	1283:1312	The genome length of strain 21Sc5-5T was 4.67 Mb containing 372 contigs and with a DNA G+C content of 70.4 mol%.
31535960	8	33	contain	containing	1291:1300	arg1	length					1253:1258	The genome length	1242:1258	The genome length of strain 21Sc5-5T	1242:1277	The genome length of strain 21Sc5-5T was 4.67 Mb containing 372 contigs and with a DNA G+C content of 70.4 mol%.
31535960	9	34	theme	phenotypic	1395:1404	arg1	analyses					1425:1432	phylogenetic, phenotypic and chemotaxonomic analyses	1381:1432	phylogenetic, phenotypic and chemotaxonomic analyses	1381:1432	On the basis of data from phylogenetic, phenotypic and chemotaxonomic analyses, strain 21Sc5-5T represents a novel species of the genus Nocardioides, for which the name Nocardioides vastitatis sp.
31535960	8	35	theme	genome	1246:1251	arg1	4.67 Mb					1283:1289	4.67 Mb	1283:1289	4.67 Mb containing 372 contigs	1283:1312	The genome length of strain 21Sc5-5T was 4.67 Mb containing 372 contigs and with a DNA G+C content of 70.4 mol%.
31535960	8	35	theme	genome	1246:1251	arg1	length					1253:1258	The genome length	1242:1258	The genome length of strain 21Sc5-5T	1242:1277	The genome length of strain 21Sc5-5T was 4.67 Mb containing 372 contigs and with a DNA G+C content of 70.4 mol%.
31535960	2	36	attach	isolated	176:183	arg2	actinobacterium					127:141	A Gram-stain-positive, aerobic, non-motile, rod-shaped actinobacterium	72:141	A Gram-stain-positive, aerobic, non-motile, rod-shaped actinobacterium	72:141	A Gram-stain-positive, aerobic, non-motile, rod-shaped actinobacterium, designated strain 21Sc5-5T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China and investigated by using a polyphasic approach.
31535960	2	36	attach	isolated	176:183	arg1	sample					197:202	a soil sample	190:202	a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China	190:283	A Gram-stain-positive, aerobic, non-motile, rod-shaped actinobacterium, designated strain 21Sc5-5T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China and investigated by using a polyphasic approach.
31535960	4	37	theme	genus	596:600	arg1	Nocardioides					602:613	the genus Nocardioides	592:613	the genus Nocardioides	592:613	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain 21Sc5-5T formed a distinct lineage within the genus Nocardioides and had the highest similarity to Nocardioides albidus THG-S11.7T (97.30 %), followed by Nocardioides kongjuensis A2-4T (97.22 %), Nocardioides nitrophenolicus NSP 41T (97.15 %) and Nocardioides caeni MN8T (97.15 %).
31535960	1	38	theme	desert	59:64	arg1	soil					66:69	Taklamakan desert soil	48:69	Taklamakan desert soil	48:69	nov., isolated from Taklamakan desert soil.
31535960	5	39	theme	diagnostic	935:944	arg1	acid					923:926	ll-diaminopimelic acid	905:926	ll-diaminopimelic acid	905:926	The results of chemotaxonomic analyses showed that the isolate possessed ll-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan and MK-8(H4) as the predominant menaquinone.
31535960	5	39	theme	diagnostic	935:944	arg1	acid					954:957	the diagnostic diamino acid	931:957	the diagnostic diamino acid of the peptidoglycan and MK-8(H4) as the predominant menaquinone	931:1022	The results of chemotaxonomic analyses showed that the isolate possessed ll-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan and MK-8(H4) as the predominant menaquinone.
31535960	2	40	theme	Taklamakan	221:230	arg1	desert					232:237	the Taklamakan desert	217:237	the Taklamakan desert	217:237	A Gram-stain-positive, aerobic, non-motile, rod-shaped actinobacterium, designated strain 21Sc5-5T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China and investigated by using a polyphasic approach.
31535960	7	41	theme	major	1184:1188	arg1	acids					1196:1200	The major fatty acids	1180:1200	The major fatty acids	1180:1200	The major fatty acids were iso-C16 : 0 and 10-methyl C18 : 0.
31535960	7	41	theme	major	1184:1188	arg1	iso-C16 					1207:1214	iso-C16 	1207:1214	iso-C16 	1207:1214	The major fatty acids were iso-C16 : 0 and 10-methyl C18 : 0.
31535960	4	42	dep	Nocardioides	746:757	arg1	41T					779:781	NSP 41T	775:781	Nocardioides nitrophenolicus NSP 41T (97.15 %)	746:791	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain 21Sc5-5T formed a distinct lineage within the genus Nocardioides and had the highest similarity to Nocardioides albidus THG-S11.7T (97.30 %), followed by Nocardioides kongjuensis A2-4T (97.22 %), Nocardioides nitrophenolicus NSP 41T (97.15 %) and Nocardioides caeni MN8T (97.15 %).
31535960	4	42	dep	Nocardioides	746:757	arg1	%					790:790	97.15 %	784:790	97.15 %	784:790	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain 21Sc5-5T formed a distinct lineage within the genus Nocardioides and had the highest similarity to Nocardioides albidus THG-S11.7T (97.30 %), followed by Nocardioides kongjuensis A2-4T (97.22 %), Nocardioides nitrophenolicus NSP 41T (97.15 %) and Nocardioides caeni MN8T (97.15 %).
31535960	4	42	dep	Nocardioides	746:757	arg1	nitrophenolicus					759:773	Nocardioides nitrophenolicus NSP 41T (97.15 %)	746:791	Nocardioides nitrophenolicus NSP 41T (97.15 %)	746:791	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain 21Sc5-5T formed a distinct lineage within the genus Nocardioides and had the highest similarity to Nocardioides albidus THG-S11.7T (97.30 %), followed by Nocardioides kongjuensis A2-4T (97.22 %), Nocardioides nitrophenolicus NSP 41T (97.15 %) and Nocardioides caeni MN8T (97.15 %).
31535960	5	43	dep	peptidoglycan	966:978	arg1	the					962:964	the	962:964	the	962:964	The results of chemotaxonomic analyses showed that the isolate possessed ll-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan and MK-8(H4) as the predominant menaquinone.
31535960	5	44	theme	diamino	946:952	arg1	acid					923:926	ll-diaminopimelic acid	905:926	ll-diaminopimelic acid	905:926	The results of chemotaxonomic analyses showed that the isolate possessed ll-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan and MK-8(H4) as the predominant menaquinone.
31535960	5	44	theme	diamino	946:952	arg1	acid					954:957	the diagnostic diamino acid	931:957	the diagnostic diamino acid of the peptidoglycan and MK-8(H4) as the predominant menaquinone	931:1022	The results of chemotaxonomic analyses showed that the isolate possessed ll-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan and MK-8(H4) as the predominant menaquinone.
31535960	7	45	theme	fatty	1190:1194	arg1	acids					1196:1200	The major fatty acids	1180:1200	The major fatty acids	1180:1200	The major fatty acids were iso-C16 : 0 and 10-methyl C18 : 0.
31535960	7	45	theme	fatty	1190:1194	arg1	iso-C16 					1207:1214	iso-C16 	1207:1214	iso-C16 	1207:1214	The major fatty acids were iso-C16 : 0 and 10-methyl C18 : 0.
31535960	5	46	theme	ll-diaminopimelic	905:921	arg1	acid					923:926	ll-diaminopimelic acid	905:926	ll-diaminopimelic acid	905:926	The results of chemotaxonomic analyses showed that the isolate possessed ll-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan and MK-8(H4) as the predominant menaquinone.
31535960	5	46	theme	ll-diaminopimelic	905:921	arg1	acid					954:957	the diagnostic diamino acid	931:957	the diagnostic diamino acid of the peptidoglycan and MK-8(H4) as the predominant menaquinone	931:1022	The results of chemotaxonomic analyses showed that the isolate possessed ll-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan and MK-8(H4) as the predominant menaquinone.
31535960	6	47	theme	polar	1029:1033	arg1	lipids					1035:1040	The polar lipids	1025:1040	The polar lipids	1025:1040	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, an unidentified phospholipid and three unidentified lipids.
31535960	5	48	contain	possessed	895:903	arg2	acid					954:957	the diagnostic diamino acid	931:957	the diagnostic diamino acid of the peptidoglycan and MK-8(H4) as the predominant menaquinone	931:1022	The results of chemotaxonomic analyses showed that the isolate possessed ll-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan and MK-8(H4) as the predominant menaquinone.
31535960	5	48	contain	possessed	895:903	arg2	acid					923:926	ll-diaminopimelic acid	905:926	ll-diaminopimelic acid	905:926	The results of chemotaxonomic analyses showed that the isolate possessed ll-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan and MK-8(H4) as the predominant menaquinone.
31535960	5	48	contain	possessed	895:903	arg1	isolate					887:893	the isolate	883:893	the isolate	883:893	The results of chemotaxonomic analyses showed that the isolate possessed ll-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan and MK-8(H4) as the predominant menaquinone.
31535960	2	49	theme	Gram-stain-positive	74:92	arg1	actinobacterium					127:141	A Gram-stain-positive, aerobic, non-motile, rod-shaped actinobacterium	72:141	A Gram-stain-positive, aerobic, non-motile, rod-shaped actinobacterium	72:141	A Gram-stain-positive, aerobic, non-motile, rod-shaped actinobacterium, designated strain 21Sc5-5T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China and investigated by using a polyphasic approach.
31535960	4	50	theme	NSP	775:777	arg1	41T					779:781	NSP 41T	775:781	Nocardioides nitrophenolicus NSP 41T (97.15 %)	746:791	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain 21Sc5-5T formed a distinct lineage within the genus Nocardioides and had the highest similarity to Nocardioides albidus THG-S11.7T (97.30 %), followed by Nocardioides kongjuensis A2-4T (97.22 %), Nocardioides nitrophenolicus NSP 41T (97.15 %) and Nocardioides caeni MN8T (97.15 %).
31535960	4	50	theme	NSP	775:777	arg1	%					790:790	97.15 %	784:790	97.15 %	784:790	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain 21Sc5-5T formed a distinct lineage within the genus Nocardioides and had the highest similarity to Nocardioides albidus THG-S11.7T (97.30 %), followed by Nocardioides kongjuensis A2-4T (97.22 %), Nocardioides nitrophenolicus NSP 41T (97.15 %) and Nocardioides caeni MN8T (97.15 %).
31535960	9	51	theme	phylogenetic	1381:1392	arg1	analyses					1425:1432	phylogenetic, phenotypic and chemotaxonomic analyses	1381:1432	phylogenetic, phenotypic and chemotaxonomic analyses	1381:1432	On the basis of data from phylogenetic, phenotypic and chemotaxonomic analyses, strain 21Sc5-5T represents a novel species of the genus Nocardioides, for which the name Nocardioides vastitatis sp.
31535960	3	52	dep	optimum	400:406	arg1	pH					409:410	pH 7.0	409:414	pH 7.0	409:414	Strain 21Sc5-5T grew at 10-37 °C (optimum, 28-30 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-3 % (w/v) NaCl (optimum, 0 %).
31535960	2	53	theme	polyphasic	313:322	arg1	approach					324:331	a polyphasic approach	311:331	a polyphasic approach	311:331	A Gram-stain-positive, aerobic, non-motile, rod-shaped actinobacterium, designated strain 21Sc5-5T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China and investigated by using a polyphasic approach.
31535960	8	54	with	4.67 Mb	1283:1289	arg1	content					1333:1339	a DNA G+C content	1323:1339	a DNA G+C content of 70.4 mol%	1323:1352	The genome length of strain 21Sc5-5T was 4.67 Mb containing 372 contigs and with a DNA G+C content of 70.4 mol%.
31535960	2	55	theme	soil	192:195	arg1	sample					197:202	a soil sample	190:202	a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China	190:283	A Gram-stain-positive, aerobic, non-motile, rod-shaped actinobacterium, designated strain 21Sc5-5T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China and investigated by using a polyphasic approach.
31535960	2	56	theme	Uygur	251:255	arg1	Region					268:273	Xinjiang Uygur Autonomous Region	242:273	Xinjiang Uygur Autonomous Region	242:273	A Gram-stain-positive, aerobic, non-motile, rod-shaped actinobacterium, designated strain 21Sc5-5T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China and investigated by using a polyphasic approach.
31535960	2	56	theme	Uygur	251:255	arg1	China					279:283	PR China	276:283	PR China	276:283	A Gram-stain-positive, aerobic, non-motile, rod-shaped actinobacterium, designated strain 21Sc5-5T, was isolated from a soil sample collected in the Taklamakan desert in Xinjiang Uygur Autonomous Region, PR China and investigated by using a polyphasic approach.
31535960	9	57	theme	genus	1485:1489	arg1	Nocardioides					1491:1502	the genus Nocardioides	1481:1502	the genus Nocardioides	1481:1502	On the basis of data from phylogenetic, phenotypic and chemotaxonomic analyses, strain 21Sc5-5T represents a novel species of the genus Nocardioides, for which the name Nocardioides vastitatis sp.
31535960	8	58	theme	70.4 mol	1344:1351	arg1	%					1352:1352	70.4 mol%	1344:1352	70.4 mol%	1344:1352	The genome length of strain 21Sc5-5T was 4.67 Mb containing 372 contigs and with a DNA G+C content of 70.4 mol%.
31535960	1	59	theme	Taklamakan	48:57	arg1	soil					66:69	Taklamakan desert soil	48:69	Taklamakan desert soil	48:69	nov., isolated from Taklamakan desert soil.
31535960	9	60	theme	vastitatis	1537:1546	arg1	sp					1548:1549	the name Nocardioides vastitatis sp	1515:1549	the name Nocardioides vastitatis sp	1515:1549	On the basis of data from phylogenetic, phenotypic and chemotaxonomic analyses, strain 21Sc5-5T represents a novel species of the genus Nocardioides, for which the name Nocardioides vastitatis sp.
31535960	8	61	theme	G+C	1329:1331	arg1	content					1333:1339	a DNA G+C content	1323:1339	a DNA G+C content of 70.4 mol%	1323:1352	The genome length of strain 21Sc5-5T was 4.67 Mb containing 372 contigs and with a DNA G+C content of 70.4 mol%.
31535960	8	62	theme	DNA	1325:1327	arg1	content					1333:1339	a DNA G+C content	1323:1339	a DNA G+C content of 70.4 mol%	1323:1352	The genome length of strain 21Sc5-5T was 4.67 Mb containing 372 contigs and with a DNA G+C content of 70.4 mol%.
31535960	5	63	theme	chemotaxonomic	847:860	arg1	analyses					862:869	chemotaxonomic analyses	847:869	chemotaxonomic analyses	847:869	The results of chemotaxonomic analyses showed that the isolate possessed ll-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan and MK-8(H4) as the predominant menaquinone.
31535960	3	64	dep	optimum	458:464	arg1	%					469:469	0 %	467:469	0 %	467:469	Strain 21Sc5-5T grew at 10-37 °C (optimum, 28-30 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-3 % (w/v) NaCl (optimum, 0 %).
31535960	9	65	from	analyses	1425:1432	arg1	data					1371:1374	data	1371:1374	data from phylogenetic, phenotypic and chemotaxonomic analyses	1371:1432	On the basis of data from phylogenetic, phenotypic and chemotaxonomic analyses, strain 21Sc5-5T represents a novel species of the genus Nocardioides, for which the name Nocardioides vastitatis sp.
31535960	4	66	theme	rRNA	508:511	arg1	sequences					518:526	16S rRNA gene sequences	504:526	16S rRNA gene sequences	504:526	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain 21Sc5-5T formed a distinct lineage within the genus Nocardioides and had the highest similarity to Nocardioides albidus THG-S11.7T (97.30 %), followed by Nocardioides kongjuensis A2-4T (97.22 %), Nocardioides nitrophenolicus NSP 41T (97.15 %) and Nocardioides caeni MN8T (97.15 %).
31535960	11	67	theme	=JCM	1599:1602	arg1	21Sc5-5T					1589:1596	21Sc5-5T	1589:1596	21Sc5-5T (=JCM 33365T=CGMCC 4.7608T)	1589:1624	The type strain is 21Sc5-5T (=JCM 33365T=CGMCC 4.7608T).
31535960	11	67	theme	=JCM	1599:1602	arg1	4.7608T					1617:1623	=JCM 33365T=CGMCC 4.7608T	1599:1623	=JCM 33365T=CGMCC 4.7608T	1599:1623	The type strain is 21Sc5-5T (=JCM 33365T=CGMCC 4.7608T).
31535960	3	68	theme	NaCl	452:455	arg1	presence					428:435	the presence	424:435	the presence of 0-3 % (w/v) NaCl (optimum, 0 %)	424:470	Strain 21Sc5-5T grew at 10-37 °C (optimum, 28-30 °C), pH 6.0-9.0 (optimum, pH 7.0) and in the presence of 0-3 % (w/v) NaCl (optimum, 0 %).
31535960	5	69	theme	analyses	862:869	arg1	results					836:842	The results	832:842	The results of chemotaxonomic analyses	832:869	The results of chemotaxonomic analyses showed that the isolate possessed ll-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan and MK-8(H4) as the predominant menaquinone.
31535960	4	70	theme	distinct	568:575	arg1	lineage					577:583	a distinct lineage	566:583	a distinct lineage	566:583	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain 21Sc5-5T formed a distinct lineage within the genus Nocardioides and had the highest similarity to Nocardioides albidus THG-S11.7T (97.30 %), followed by Nocardioides kongjuensis A2-4T (97.22 %), Nocardioides nitrophenolicus NSP 41T (97.15 %) and Nocardioides caeni MN8T (97.15 %).
31535960	9	71	theme	novel	1464:1468	arg1	species					1470:1476	a novel species	1462:1476	a novel species	1462:1476	On the basis of data from phylogenetic, phenotypic and chemotaxonomic analyses, strain 21Sc5-5T represents a novel species of the genus Nocardioides, for which the name Nocardioides vastitatis sp.
31535960	4	72	theme	16S	504:506	arg1	sequences					518:526	16S rRNA gene sequences	504:526	16S rRNA gene sequences	504:526	Phylogenetic analysis based on 16S rRNA gene sequences suggested that strain 21Sc5-5T formed a distinct lineage within the genus Nocardioides and had the highest similarity to Nocardioides albidus THG-S11.7T (97.30 %), followed by Nocardioides kongjuensis A2-4T (97.22 %), Nocardioides nitrophenolicus NSP 41T (97.15 %) and Nocardioides caeni MN8T (97.15 %).
33006213	8	0	theme	resident	1438:1445	arg1	microbiome					1447:1456	the resident microbiome	1434:1456	the resident microbiome	1434:1456	The transfer of anti-PM_CBP antibodies into the tick gut impacted the overall composition of the resident microbiome, and also influenced B. burgdorferi acquisition in ticks and its transmission to mice.
33006213	9	1	theme	biological	1586:1595	arg1	significance					1597:1608	the biological significance	1582:1608	the biological significance of the Ixodes PM	1582:1625	Taken together, these data highlight the biological significance of the Ixodes PM and suggest that the targeting of its molecular constituents may contribute to the development of novel interventions against tick-borne infections.
33006213	4	2	theme	Binding	676:682	arg1	PM_CBP					693:698	PM_CBP	693:698	PM_CBP	693:698	Herein, we characterise a novel PM-associated gut protein in Ixodes scapularis ticks, annotated as Peritrophic Membrane Chitin Binding Protein (PM_CBP), for its role in the integrity and function of the matrix.
33006213	4	2	theme	Binding	676:682	arg1	Protein					684:690	Peritrophic Membrane Chitin Binding Protein	648:690	Peritrophic Membrane Chitin Binding Protein (PM_CBP)	648:699	Herein, we characterise a novel PM-associated gut protein in Ixodes scapularis ticks, annotated as Peritrophic Membrane Chitin Binding Protein (PM_CBP), for its role in the integrity and function of the matrix.
33006213	8	3	theme	tick	1389:1392	arg1	gut					1394:1396	the tick gut	1385:1396	the tick gut	1385:1396	The transfer of anti-PM_CBP antibodies into the tick gut impacted the overall composition of the resident microbiome, and also influenced B. burgdorferi acquisition in ticks and its transmission to mice.
33006213	9	4	theme	tick-borne	1753:1762	arg1	infections					1764:1773	tick-borne infections	1753:1773	tick-borne infections	1753:1773	Taken together, these data highlight the biological significance of the Ixodes PM and suggest that the targeting of its molecular constituents may contribute to the development of novel interventions against tick-borne infections.
33006213	5	5	located	localized	877:885	arg1	surface					902:908	the luminal surface	890:908	the luminal surface of the gut epithelium	890:930	The PM_CBP displays homology to the chitin deacetylase metalloenzyme, shows upregulation during tick feeding, and is localized at the luminal surface of the gut epithelium.
33006213	5	5	located	localized	877:885	arg2	PM_CBP					764:769	The PM_CBP	760:769	The PM_CBP	760:769	The PM_CBP displays homology to the chitin deacetylase metalloenzyme, shows upregulation during tick feeding, and is localized at the luminal surface of the gut epithelium.
33006213	5	6	theme	tick	856:859	arg1	feeding					861:867	tick feeding	856:867	tick feeding	856:867	The PM_CBP displays homology to the chitin deacetylase metalloenzyme, shows upregulation during tick feeding, and is localized at the luminal surface of the gut epithelium.
33006213	2	7	theme	important	310:318	arg1	role					320:323	an important role	307:323	an important role	307:323	The structure is thought to play an important role in tick biology.
33006213	6	8	theme	structural	937:946	arg1	integrity					948:956	The structural integrity	933:956	The structural integrity of the PM	933:966	The structural integrity of the PM was impaired both by the knock down of PM_CBP expression via RNA interference and by treatment with anti-PM_CBP antibodies, as revealed by its electron microscopic appearance.
33006213	0	9	theme	gut	89:91	arg1	microbiome					93:102	gut microbiome	89:102	gut microbiome	89:102	A novel tick protein supports integrity of gut peritrophic matrix impacting existence of gut microbiome and Lyme disease pathogens.
33006213	6	10	with	treatment	1053:1061	arg1	antibodies					1080:1089	anti-PM_CBP antibodies	1068:1089	anti-PM_CBP antibodies	1068:1089	The structural integrity of the PM was impaired both by the knock down of PM_CBP expression via RNA interference and by treatment with anti-PM_CBP antibodies, as revealed by its electron microscopic appearance.
33006213	7	11	from	engorgement	1179:1189	arg1	mice					1194:1197	mice	1194:1197	mice	1194:1197	Additionally, the duration of tick engorgement on mice and the passage of experimentally-inoculated fluorescent dextran molecules across the PM are affected by the knock down of PM_CBP expression.
33006213	6	12	theme	electron	1111:1118	arg1	appearance					1132:1141	its electron microscopic appearance	1107:1141	its electron microscopic appearance	1107:1141	The structural integrity of the PM was impaired both by the knock down of PM_CBP expression via RNA interference and by treatment with anti-PM_CBP antibodies, as revealed by its electron microscopic appearance.
33006213	6	13	theme	anti-PM_CBP	1068:1078	arg1	antibodies					1080:1089	anti-PM_CBP antibodies	1068:1089	anti-PM_CBP antibodies	1068:1089	The structural integrity of the PM was impaired both by the knock down of PM_CBP expression via RNA interference and by treatment with anti-PM_CBP antibodies, as revealed by its electron microscopic appearance.
33006213	9	14	theme	novel	1725:1729	arg1	interventions					1731:1743	novel interventions	1725:1743	novel interventions against tick-borne infections	1725:1773	Taken together, these data highlight the biological significance of the Ixodes PM and suggest that the targeting of its molecular constituents may contribute to the development of novel interventions against tick-borne infections.
33006213	8	15	from	acquisition	1494:1504	arg1	ticks					1509:1513	ticks	1509:1513	ticks	1509:1513	The transfer of anti-PM_CBP antibodies into the tick gut impacted the overall composition of the resident microbiome, and also influenced B. burgdorferi acquisition in ticks and its transmission to mice.
33006213	8	15	from	acquisition	1494:1504	arg1	transmission					1523:1534	its transmission	1519:1534	its transmission to mice	1519:1542	The transfer of anti-PM_CBP antibodies into the tick gut impacted the overall composition of the resident microbiome, and also influenced B. burgdorferi acquisition in ticks and its transmission to mice.
33006213	7	16	theme	engorgement	1179:1189	arg1	passage					1207:1213	the passage	1203:1213	the passage of experimentally-inoculated fluorescent dextran molecules across the PM	1203:1286	Additionally, the duration of tick engorgement on mice and the passage of experimentally-inoculated fluorescent dextran molecules across the PM are affected by the knock down of PM_CBP expression.
33006213	7	16	theme	engorgement	1179:1189	arg1	duration					1162:1169	the duration	1158:1169	the duration of tick engorgement on mice	1158:1197	Additionally, the duration of tick engorgement on mice and the passage of experimentally-inoculated fluorescent dextran molecules across the PM are affected by the knock down of PM_CBP expression.
33006213	0	17	dep	microbiome	93:102	arg1	pathogens					121:129	pathogens	121:129	pathogens	121:129	A novel tick protein supports integrity of gut peritrophic matrix impacting existence of gut microbiome and Lyme disease pathogens.
33006213	4	18	theme	Chitin	669:674	arg1	PM_CBP					693:698	PM_CBP	693:698	PM_CBP	693:698	Herein, we characterise a novel PM-associated gut protein in Ixodes scapularis ticks, annotated as Peritrophic Membrane Chitin Binding Protein (PM_CBP), for its role in the integrity and function of the matrix.
33006213	4	18	theme	Chitin	669:674	arg1	Protein					684:690	Peritrophic Membrane Chitin Binding Protein	648:690	Peritrophic Membrane Chitin Binding Protein (PM_CBP)	648:699	Herein, we characterise a novel PM-associated gut protein in Ixodes scapularis ticks, annotated as Peritrophic Membrane Chitin Binding Protein (PM_CBP), for its role in the integrity and function of the matrix.
33006213	9	19	theme	Ixodes	1617:1622	arg1	PM					1624:1625	the Ixodes PM	1613:1625	the Ixodes PM	1613:1625	Taken together, these data highlight the biological significance of the Ixodes PM and suggest that the targeting of its molecular constituents may contribute to the development of novel interventions against tick-borne infections.
33006213	7	20	theme	molecules	1264:1272	arg1	passage					1207:1213	the passage	1203:1213	the passage of experimentally-inoculated fluorescent dextran molecules across the PM	1203:1286	Additionally, the duration of tick engorgement on mice and the passage of experimentally-inoculated fluorescent dextran molecules across the PM are affected by the knock down of PM_CBP expression.
33006213	7	20	theme	molecules	1264:1272	arg1	duration					1162:1169	the duration	1158:1169	the duration of tick engorgement on mice	1158:1197	Additionally, the duration of tick engorgement on mice and the passage of experimentally-inoculated fluorescent dextran molecules across the PM are affected by the knock down of PM_CBP expression.
33006213	5	21	theme	gut	917:919	arg1	epithelium					921:930	the gut epithelium	913:930	the gut epithelium	913:930	The PM_CBP displays homology to the chitin deacetylase metalloenzyme, shows upregulation during tick feeding, and is localized at the luminal surface of the gut epithelium.
33006213	5	22	theme	deacetylase	803:813	arg1	metalloenzyme					815:827	the chitin deacetylase metalloenzyme	792:827	the chitin deacetylase metalloenzyme	792:827	The PM_CBP displays homology to the chitin deacetylase metalloenzyme, shows upregulation during tick feeding, and is localized at the luminal surface of the gut epithelium.
33006213	0	23	theme	microbiome	93:102	arg1	existence					76:84	existence	76:84	existence of gut microbiome and Lyme disease pathogens	76:129	A novel tick protein supports integrity of gut peritrophic matrix impacting existence of gut microbiome and Lyme disease pathogens.
33006213	8	24	theme	burgdorferi	1482:1492	arg1	acquisition					1494:1504	B. burgdorferi acquisition	1479:1504	B. burgdorferi acquisition in ticks and its transmission to mice	1479:1542	The transfer of anti-PM_CBP antibodies into the tick gut impacted the overall composition of the resident microbiome, and also influenced B. burgdorferi acquisition in ticks and its transmission to mice.
33006213	9	25	theme	PM	1624:1625	arg1	significance					1597:1608	the biological significance	1582:1608	the biological significance of the Ixodes PM	1582:1625	Taken together, these data highlight the biological significance of the Ixodes PM and suggest that the targeting of its molecular constituents may contribute to the development of novel interventions against tick-borne infections.
33006213	4	26	from	role	710:713	arg1	function					736:743	function	736:743	function	736:743	Herein, we characterise a novel PM-associated gut protein in Ixodes scapularis ticks, annotated as Peritrophic Membrane Chitin Binding Protein (PM_CBP), for its role in the integrity and function of the matrix.
33006213	4	26	from	role	710:713	arg1	integrity					722:730	integrity	722:730	integrity	722:730	Herein, we characterise a novel PM-associated gut protein in Ixodes scapularis ticks, annotated as Peritrophic Membrane Chitin Binding Protein (PM_CBP), for its role in the integrity and function of the matrix.
33006213	3	27	theme	molecular	492:500	arg1	constituents					502:513	its molecular constituents	488:513	its molecular constituents	488:513	The PM is also known to impact the persistence of tick-borne pathogens like Borrelia burgdorferi, although limited information is available about its molecular constituents or their biological significance.
33006213	6	28	theme	expression	1014:1023	arg1	down					999:1002	the knock down	989:1002	the knock down of PM_CBP expression via RNA interference	989:1044	The structural integrity of the PM was impaired both by the knock down of PM_CBP expression via RNA interference and by treatment with anti-PM_CBP antibodies, as revealed by its electron microscopic appearance.
33006213	9	29	theme	interventions	1731:1743	arg1	development					1710:1720	the development	1706:1720	the development of novel interventions against tick-borne infections	1706:1773	Taken together, these data highlight the biological significance of the Ixodes PM and suggest that the targeting of its molecular constituents may contribute to the development of novel interventions against tick-borne infections.
33006213	0	30	theme	novel	2:6	arg1	protein					13:19	A novel tick protein	0:19	A novel tick protein	0:19	A novel tick protein supports integrity of gut peritrophic matrix impacting existence of gut microbiome and Lyme disease pathogens.
33006213	8	31	theme	anti-PM_CBP	1357:1367	arg1	antibodies					1369:1378	anti-PM_CBP antibodies	1357:1378	anti-PM_CBP antibodies	1357:1378	The transfer of anti-PM_CBP antibodies into the tick gut impacted the overall composition of the resident microbiome, and also influenced B. burgdorferi acquisition in ticks and its transmission to mice.
33006213	7	32	theme	experimentally-inoculated	1218:1242	arg1	molecules					1264:1272	experimentally-inoculated fluorescent dextran molecules	1218:1272	experimentally-inoculated fluorescent dextran molecules	1218:1272	Additionally, the duration of tick engorgement on mice and the passage of experimentally-inoculated fluorescent dextran molecules across the PM are affected by the knock down of PM_CBP expression.
33006213	4	33	dep	integrity	722:730	arg1	the					718:720	the	718:720	the	718:720	Herein, we characterise a novel PM-associated gut protein in Ixodes scapularis ticks, annotated as Peritrophic Membrane Chitin Binding Protein (PM_CBP), for its role in the integrity and function of the matrix.
33006213	7	34	from	passage	1207:1213	arg1	mice					1194:1197	mice	1194:1197	mice	1194:1197	Additionally, the duration of tick engorgement on mice and the passage of experimentally-inoculated fluorescent dextran molecules across the PM are affected by the knock down of PM_CBP expression.
33006213	6	35	theme	knock	993:997	arg1	down					999:1002	the knock down	989:1002	the knock down of PM_CBP expression via RNA interference	989:1044	The structural integrity of the PM was impaired both by the knock down of PM_CBP expression via RNA interference and by treatment with anti-PM_CBP antibodies, as revealed by its electron microscopic appearance.
33006213	0	36	theme	disease	113:119	arg1	existence					76:84	existence	76:84	existence of gut microbiome and Lyme disease pathogens	76:129	A novel tick protein supports integrity of gut peritrophic matrix impacting existence of gut microbiome and Lyme disease pathogens.
33006213	7	37	from	mice	1194:1197	arg1	passage					1207:1213	the passage	1203:1213	the passage of experimentally-inoculated fluorescent dextran molecules across the PM	1203:1286	Additionally, the duration of tick engorgement on mice and the passage of experimentally-inoculated fluorescent dextran molecules across the PM are affected by the knock down of PM_CBP expression.
33006213	7	37	from	mice	1194:1197	arg1	duration					1162:1169	the duration	1158:1169	the duration of tick engorgement on mice	1158:1197	Additionally, the duration of tick engorgement on mice and the passage of experimentally-inoculated fluorescent dextran molecules across the PM are affected by the knock down of PM_CBP expression.
33006213	5	38	theme	luminal	894:900	arg1	surface					902:908	the luminal surface	890:908	the luminal surface of the gut epithelium	890:930	The PM_CBP displays homology to the chitin deacetylase metalloenzyme, shows upregulation during tick feeding, and is localized at the luminal surface of the gut epithelium.
33006213	1	39	theme	acellular	166:174	arg1	membrane					176:183	an acellular membrane	163:183	an acellular membrane that covers the gut epithelium in arthropods and physically separates it from the lumen	163:271	The peritrophic matrix (PM) is an acellular membrane that covers the gut epithelium in arthropods and physically separates it from the lumen.
33006213	1	39	theme	acellular	166:174	arg1	matrix					148:153	The peritrophic matrix	132:153	The peritrophic matrix (PM)	132:158	The peritrophic matrix (PM) is an acellular membrane that covers the gut epithelium in arthropods and physically separates it from the lumen.
33006213	0	40	theme	tick	8:11	arg1	protein					13:19	A novel tick protein	0:19	A novel tick protein	0:19	A novel tick protein supports integrity of gut peritrophic matrix impacting existence of gut microbiome and Lyme disease pathogens.
33006213	0	41	theme	Lyme	108:111	arg1	disease					113:119	Lyme disease	108:119	Lyme disease	108:119	A novel tick protein supports integrity of gut peritrophic matrix impacting existence of gut microbiome and Lyme disease pathogens.
33006213	3	42	theme	biological	524:533	arg1	significance					535:546	their biological significance	518:546	their biological significance	518:546	The PM is also known to impact the persistence of tick-borne pathogens like Borrelia burgdorferi, although limited information is available about its molecular constituents or their biological significance.
33006213	4	43	theme	Membrane	660:667	arg1	PM_CBP					693:698	PM_CBP	693:698	PM_CBP	693:698	Herein, we characterise a novel PM-associated gut protein in Ixodes scapularis ticks, annotated as Peritrophic Membrane Chitin Binding Protein (PM_CBP), for its role in the integrity and function of the matrix.
33006213	4	43	theme	Membrane	660:667	arg1	Protein					684:690	Peritrophic Membrane Chitin Binding Protein	648:690	Peritrophic Membrane Chitin Binding Protein (PM_CBP)	648:699	Herein, we characterise a novel PM-associated gut protein in Ixodes scapularis ticks, annotated as Peritrophic Membrane Chitin Binding Protein (PM_CBP), for its role in the integrity and function of the matrix.
33006213	7	44	theme	fluorescent	1244:1254	arg1	molecules					1264:1272	experimentally-inoculated fluorescent dextran molecules	1218:1272	experimentally-inoculated fluorescent dextran molecules	1218:1272	Additionally, the duration of tick engorgement on mice and the passage of experimentally-inoculated fluorescent dextran molecules across the PM are affected by the knock down of PM_CBP expression.
33006213	4	45	theme	scapularis	617:626	arg1	ticks					628:632	Ixodes scapularis ticks	610:632	Ixodes scapularis ticks	610:632	Herein, we characterise a novel PM-associated gut protein in Ixodes scapularis ticks, annotated as Peritrophic Membrane Chitin Binding Protein (PM_CBP), for its role in the integrity and function of the matrix.
33006213	7	46	theme	PM_CBP	1322:1327	arg1	expression					1329:1338	PM_CBP expression	1322:1338	PM_CBP expression	1322:1338	Additionally, the duration of tick engorgement on mice and the passage of experimentally-inoculated fluorescent dextran molecules across the PM are affected by the knock down of PM_CBP expression.
33006213	4	47	from	protein	599:605	arg1	ticks					628:632	Ixodes scapularis ticks	610:632	Ixodes scapularis ticks	610:632	Herein, we characterise a novel PM-associated gut protein in Ixodes scapularis ticks, annotated as Peritrophic Membrane Chitin Binding Protein (PM_CBP), for its role in the integrity and function of the matrix.
33006213	3	48	theme	tick-borne	392:401	arg1	pathogens					403:411	tick-borne pathogens	392:411	tick-borne pathogens like Borrelia burgdorferi	392:437	The PM is also known to impact the persistence of tick-borne pathogens like Borrelia burgdorferi, although limited information is available about its molecular constituents or their biological significance.
33006213	6	49	theme	PM_CBP	1007:1012	arg1	expression					1014:1023	PM_CBP expression	1007:1023	PM_CBP expression	1007:1023	The structural integrity of the PM was impaired both by the knock down of PM_CBP expression via RNA interference and by treatment with anti-PM_CBP antibodies, as revealed by its electron microscopic appearance.
33006213	7	50	theme	expression	1329:1338	arg1	down					1314:1317	the knock down	1304:1317	the knock down of PM_CBP expression	1304:1338	Additionally, the duration of tick engorgement on mice and the passage of experimentally-inoculated fluorescent dextran molecules across the PM are affected by the knock down of PM_CBP expression.
33006213	5	51	theme	chitin	796:801	arg1	metalloenzyme					815:827	the chitin deacetylase metalloenzyme	792:827	the chitin deacetylase metalloenzyme	792:827	The PM_CBP displays homology to the chitin deacetylase metalloenzyme, shows upregulation during tick feeding, and is localized at the luminal surface of the gut epithelium.
33006213	3	52	theme	pathogens	403:411	arg1	persistence					377:387	the persistence	373:387	the persistence of tick-borne pathogens like Borrelia burgdorferi	373:437	The PM is also known to impact the persistence of tick-borne pathogens like Borrelia burgdorferi, although limited information is available about its molecular constituents or their biological significance.
33006213	0	53	theme	gut	43:45	arg1	matrix					59:64	gut peritrophic matrix	43:64	gut peritrophic matrix impacting existence of gut microbiome and Lyme disease pathogens	43:129	A novel tick protein supports integrity of gut peritrophic matrix impacting existence of gut microbiome and Lyme disease pathogens.
33006213	1	54	from	epithelium	205:214	arg1	arthropods					219:228	arthropods	219:228	arthropods	219:228	The peritrophic matrix (PM) is an acellular membrane that covers the gut epithelium in arthropods and physically separates it from the lumen.
33006213	6	55	theme	RNA	1029:1031	arg1	interference					1033:1044	RNA interference	1029:1044	RNA interference	1029:1044	The structural integrity of the PM was impaired both by the knock down of PM_CBP expression via RNA interference and by treatment with anti-PM_CBP antibodies, as revealed by its electron microscopic appearance.
33006213	9	56	theme	molecular	1665:1673	arg1	constituents					1675:1686	its molecular constituents	1661:1686	its molecular constituents	1661:1686	Taken together, these data highlight the biological significance of the Ixodes PM and suggest that the targeting of its molecular constituents may contribute to the development of novel interventions against tick-borne infections.
33006213	3	57	theme	limited	449:455	arg1	information					457:467	limited information	449:467	limited information	449:467	The PM is also known to impact the persistence of tick-borne pathogens like Borrelia burgdorferi, although limited information is available about its molecular constituents or their biological significance.
33006213	9	58	theme	constituents	1675:1686	arg1	targeting					1648:1656	the targeting	1644:1656	the targeting of its molecular constituents	1644:1686	Taken together, these data highlight the biological significance of the Ixodes PM and suggest that the targeting of its molecular constituents may contribute to the development of novel interventions against tick-borne infections.
33006213	8	59	theme	overall	1411:1417	arg1	composition					1419:1429	the overall composition	1407:1429	the overall composition of the resident microbiome	1407:1456	The transfer of anti-PM_CBP antibodies into the tick gut impacted the overall composition of the resident microbiome, and also influenced B. burgdorferi acquisition in ticks and its transmission to mice.
33006213	8	60	theme	antibodies	1369:1378	arg1	transfer					1345:1352	The transfer	1341:1352	The transfer of anti-PM_CBP antibodies into the tick gut	1341:1396	The transfer of anti-PM_CBP antibodies into the tick gut impacted the overall composition of the resident microbiome, and also influenced B. burgdorferi acquisition in ticks and its transmission to mice.
33006213	6	61	theme	PM	965:966	arg1	integrity					948:956	The structural integrity	933:956	The structural integrity of the PM	933:966	The structural integrity of the PM was impaired both by the knock down of PM_CBP expression via RNA interference and by treatment with anti-PM_CBP antibodies, as revealed by its electron microscopic appearance.
33006213	4	62	theme	novel	575:579	arg1	protein					599:605	a novel PM-associated gut protein	573:605	a novel PM-associated gut protein	573:605	Herein, we characterise a novel PM-associated gut protein in Ixodes scapularis ticks, annotated as Peritrophic Membrane Chitin Binding Protein (PM_CBP), for its role in the integrity and function of the matrix.
33006213	5	63	theme	epithelium	921:930	arg1	surface					902:908	the luminal surface	890:908	the luminal surface of the gut epithelium	890:930	The PM_CBP displays homology to the chitin deacetylase metalloenzyme, shows upregulation during tick feeding, and is localized at the luminal surface of the gut epithelium.
33006213	7	64	from	duration	1162:1169	arg1	mice					1194:1197	mice	1194:1197	mice	1194:1197	Additionally, the duration of tick engorgement on mice and the passage of experimentally-inoculated fluorescent dextran molecules across the PM are affected by the knock down of PM_CBP expression.
33006213	7	65	theme	knock	1308:1312	arg1	down					1314:1317	the knock down	1304:1317	the knock down of PM_CBP expression	1304:1338	Additionally, the duration of tick engorgement on mice and the passage of experimentally-inoculated fluorescent dextran molecules across the PM are affected by the knock down of PM_CBP expression.
33006213	4	66	theme	Peritrophic	648:658	arg1	PM_CBP					693:698	PM_CBP	693:698	PM_CBP	693:698	Herein, we characterise a novel PM-associated gut protein in Ixodes scapularis ticks, annotated as Peritrophic Membrane Chitin Binding Protein (PM_CBP), for its role in the integrity and function of the matrix.
33006213	4	66	theme	Peritrophic	648:658	arg1	Protein					684:690	Peritrophic Membrane Chitin Binding Protein	648:690	Peritrophic Membrane Chitin Binding Protein (PM_CBP)	648:699	Herein, we characterise a novel PM-associated gut protein in Ixodes scapularis ticks, annotated as Peritrophic Membrane Chitin Binding Protein (PM_CBP), for its role in the integrity and function of the matrix.
33006213	1	67	theme	gut	201:203	arg1	epithelium					205:214	the gut epithelium	197:214	the gut epithelium in arthropods	197:228	The peritrophic matrix (PM) is an acellular membrane that covers the gut epithelium in arthropods and physically separates it from the lumen.
33006213	0	68	theme	matrix	59:64	arg1	integrity					30:38	integrity	30:38	integrity of gut peritrophic matrix impacting existence of gut microbiome and Lyme disease pathogens	30:129	A novel tick protein supports integrity of gut peritrophic matrix impacting existence of gut microbiome and Lyme disease pathogens.
33006213	4	69	theme	Ixodes	610:615	arg1	ticks					628:632	Ixodes scapularis ticks	610:632	Ixodes scapularis ticks	610:632	Herein, we characterise a novel PM-associated gut protein in Ixodes scapularis ticks, annotated as Peritrophic Membrane Chitin Binding Protein (PM_CBP), for its role in the integrity and function of the matrix.
33006213	4	70	theme	matrix	752:757	arg1	function					736:743	function	736:743	function	736:743	Herein, we characterise a novel PM-associated gut protein in Ixodes scapularis ticks, annotated as Peritrophic Membrane Chitin Binding Protein (PM_CBP), for its role in the integrity and function of the matrix.
33006213	4	70	theme	matrix	752:757	arg1	integrity					722:730	integrity	722:730	integrity	722:730	Herein, we characterise a novel PM-associated gut protein in Ixodes scapularis ticks, annotated as Peritrophic Membrane Chitin Binding Protein (PM_CBP), for its role in the integrity and function of the matrix.
33006213	7	71	theme	dextran	1256:1262	arg1	molecules					1264:1272	experimentally-inoculated fluorescent dextran molecules	1218:1272	experimentally-inoculated fluorescent dextran molecules	1218:1272	Additionally, the duration of tick engorgement on mice and the passage of experimentally-inoculated fluorescent dextran molecules across the PM are affected by the knock down of PM_CBP expression.
33006213	2	72	theme	tick	328:331	arg1	biology					333:339	tick biology	328:339	tick biology	328:339	The structure is thought to play an important role in tick biology.
33006213	4	73	theme	gut	595:597	arg1	protein					599:605	a novel PM-associated gut protein	573:605	a novel PM-associated gut protein	573:605	Herein, we characterise a novel PM-associated gut protein in Ixodes scapularis ticks, annotated as Peritrophic Membrane Chitin Binding Protein (PM_CBP), for its role in the integrity and function of the matrix.
33006213	0	74	theme	peritrophic	47:57	arg1	matrix					59:64	gut peritrophic matrix	43:64	gut peritrophic matrix impacting existence of gut microbiome and Lyme disease pathogens	43:129	A novel tick protein supports integrity of gut peritrophic matrix impacting existence of gut microbiome and Lyme disease pathogens.
33006213	8	75	theme	microbiome	1447:1456	arg1	composition					1419:1429	the overall composition	1407:1429	the overall composition of the resident microbiome	1407:1456	The transfer of anti-PM_CBP antibodies into the tick gut impacted the overall composition of the resident microbiome, and also influenced B. burgdorferi acquisition in ticks and its transmission to mice.
33006213	6	76	theme	microscopic	1120:1130	arg1	appearance					1132:1141	its electron microscopic appearance	1107:1141	its electron microscopic appearance	1107:1141	The structural integrity of the PM was impaired both by the knock down of PM_CBP expression via RNA interference and by treatment with anti-PM_CBP antibodies, as revealed by its electron microscopic appearance.
33006213	1	77	theme	peritrophic	136:146	arg1	membrane					176:183	an acellular membrane	163:183	an acellular membrane that covers the gut epithelium in arthropods and physically separates it from the lumen	163:271	The peritrophic matrix (PM) is an acellular membrane that covers the gut epithelium in arthropods and physically separates it from the lumen.
33006213	1	77	theme	peritrophic	136:146	arg1	PM					156:157	PM	156:157	PM	156:157	The peritrophic matrix (PM) is an acellular membrane that covers the gut epithelium in arthropods and physically separates it from the lumen.
33006213	1	77	theme	peritrophic	136:146	arg1	matrix					148:153	The peritrophic matrix	132:153	The peritrophic matrix (PM)	132:158	The peritrophic matrix (PM) is an acellular membrane that covers the gut epithelium in arthropods and physically separates it from the lumen.
33006213	7	78	theme	tick	1174:1177	arg1	engorgement					1179:1189	tick engorgement	1174:1189	tick engorgement on mice	1174:1197	Additionally, the duration of tick engorgement on mice and the passage of experimentally-inoculated fluorescent dextran molecules across the PM are affected by the knock down of PM_CBP expression.
33006213	4	79	theme	PM-associated	581:593	arg1	protein					599:605	a novel PM-associated gut protein	573:605	a novel PM-associated gut protein	573:605	Herein, we characterise a novel PM-associated gut protein in Ixodes scapularis ticks, annotated as Peritrophic Membrane Chitin Binding Protein (PM_CBP), for its role in the integrity and function of the matrix.
34712749	9	0	theme	SILAC	1722:1726	arg1	pSILAC					1738:1743	pSILAC	1738:1743	pSILAC	1738:1743	In a second step we delineate the degradation kinetics of some of these proteins using a pulse SILAC strategy (pSILAC) thus complementing our initial findings with a fourth dataset.
34712749	9	0	theme	SILAC	1722:1726	arg1	strategy					1728:1735	a pulse SILAC strategy	1714:1735	a pulse SILAC strategy (pSILAC)	1714:1744	In a second step we delineate the degradation kinetics of some of these proteins using a pulse SILAC strategy (pSILAC) thus complementing our initial findings with a fourth dataset.
34712749	8	1	used	used	1499:1502	arg2	dataset					1482:1488	The same dataset	1473:1488	The same dataset	1473:1488	The same dataset was also used to define glycoproteins altered by the down-regulation of endogenous EDEM2, which should contain its candidate-substrates.
34712749	1	2	theme	misfolded	266:274	arg1	proteins					276:283	misfolded proteins	266:283	misfolded proteins from the endoplasmic reticulum	266:314	EDEM2 (Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2) is one of the key-proteins suggested to be involved in the selection and degradation of misfolded proteins from the endoplasmic reticulum.
34712749	4	3	theme	identified	929:938	arg1	partners					940:947	EDEM2 identified partners	923:947	EDEM2 identified partners	923:947	The dataset was substantiated by SDF (Sucrose Density Fractionation)-nanoLC-MS/MS experiments, in an integrated workflow to validate EDEM2 identified partners and corroborate these with previous data.
34712749	6	4	theme	melanoma	1262:1269	arg1	lysates					1276:1282	A375 melanoma cell lysates	1257:1282	A375 melanoma cell lysates	1257:1282	The first one refers to the deglycoproteomics dataset, which covers nanoLC-MS/MS analysis of Concanavalin A enriched glycopeptides released by endoglycosidase digestion from A375 melanoma cell lysates.
34712749	3	5	theme	EDEM2	763:767	arg1	proteins					780:787	novel EDEM2 associated proteins	757:787	novel EDEM2 associated proteins	757:787	Our first aim was to affinity-enrich EDEM2 alongside its potential interaction partners and analyse the obtained samples by nanoLC-MS/MS to identify novel EDEM2 associated proteins.
34712749	7	6	with	glycoproteins	1324:1336	arg1	N-glycans					1355:1363	non-matured N-glycans	1343:1363	non-matured N-glycans from A375 melanoma cells	1343:1388	This allowed us to map the fraction of glycoproteins with non-matured N-glycans from A375 melanoma cells and find or validate N-glycosylation sites of proteins from the secretory pathway.
34712749	10	7	theme	nanoLC-MS/MS	1816:1827	arg1	findings					1842:1849	Beside nanoLC-MS/MS analysis our findings	1809:1849	Beside nanoLC-MS/MS analysis our findings	1809:1849	Beside nanoLC-MS/MS analysis our findings were also validated by various biochemical experiments.
34712749	2	8	with	deglycoproteomics	449:465	arg1	expression					587:596	modified expression	578:596	modified expression of EDEM2	578:605	The datasets discussed in this article are related to experiments covering affinity proteomics, label-free quantitative proteomics, deglycoproteomics and SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data of A375 melanoma cells with modified expression of EDEM2.
34712749	6	9	theme	endoglycosidase	1226:1240	arg1	digestion					1242:1250	endoglycosidase digestion	1226:1250	endoglycosidase digestion	1226:1250	The first one refers to the deglycoproteomics dataset, which covers nanoLC-MS/MS analysis of Concanavalin A enriched glycopeptides released by endoglycosidase digestion from A375 melanoma cell lysates.
34712749	2	10	theme	modified	578:585	arg1	expression					587:596	modified expression	578:596	modified expression of EDEM2	578:605	The datasets discussed in this article are related to experiments covering affinity proteomics, label-free quantitative proteomics, deglycoproteomics and SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data of A375 melanoma cells with modified expression of EDEM2.
34712749	10	11	theme	various	1874:1880	arg1	experiments					1894:1904	various biochemical experiments	1874:1904	various biochemical experiments	1874:1904	Beside nanoLC-MS/MS analysis our findings were also validated by various biochemical experiments.
34712749	8	12	contain	contain	1593:1599	arg2	candidate-substrates					1605:1624	its candidate-substrates	1601:1624	its candidate-substrates	1601:1624	The same dataset was also used to define glycoproteins altered by the down-regulation of endogenous EDEM2, which should contain its candidate-substrates.
34712749	8	12	contain	contain	1593:1599	arg1	which					1580:1584	which	1580:1584	which	1580:1584	The same dataset was also used to define glycoproteins altered by the down-regulation of endogenous EDEM2, which should contain its candidate-substrates.
34712749	2	13	theme	cells	567:571	arg1	data					545:548	SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data	471:548	SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data	471:548	The datasets discussed in this article are related to experiments covering affinity proteomics, label-free quantitative proteomics, deglycoproteomics and SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data of A375 melanoma cells with modified expression of EDEM2.
34712749	2	13	theme	cells	567:571	arg1	proteomics					401:410	affinity proteomics	392:410	affinity proteomics	392:410	The datasets discussed in this article are related to experiments covering affinity proteomics, label-free quantitative proteomics, deglycoproteomics and SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data of A375 melanoma cells with modified expression of EDEM2.
34712749	2	13	theme	cells	567:571	arg1	deglycoproteomics					449:465	deglycoproteomics	449:465	deglycoproteomics	449:465	The datasets discussed in this article are related to experiments covering affinity proteomics, label-free quantitative proteomics, deglycoproteomics and SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data of A375 melanoma cells with modified expression of EDEM2.
34712749	9	14	theme	fourth	1793:1798	arg1	dataset					1800:1806	a fourth dataset	1791:1806	a fourth dataset	1791:1806	In a second step we delineate the degradation kinetics of some of these proteins using a pulse SILAC strategy (pSILAC) thus complementing our initial findings with a fourth dataset.
34712749	1	15	dep	selection	237:245	arg1	the					233:235	the	233:235	the	233:235	EDEM2 (Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2) is one of the key-proteins suggested to be involved in the selection and degradation of misfolded proteins from the endoplasmic reticulum.
34712749	1	16	from	degradation	251:261	arg1	reticulum					306:314	the endoplasmic reticulum	290:314	the endoplasmic reticulum	290:314	EDEM2 (Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2) is one of the key-proteins suggested to be involved in the selection and degradation of misfolded proteins from the endoplasmic reticulum.
34712749	3	17	theme	first	612:616	arg1	aim					618:620	Our first aim	608:620	Our first aim	608:620	Our first aim was to affinity-enrich EDEM2 alongside its potential interaction partners and analyse the obtained samples by nanoLC-MS/MS to identify novel EDEM2 associated proteins.
34712749	1	18	theme	Degradation-Enhancing	122:142	arg1	EDEM2					93:97	EDEM2	93:97	EDEM2 (Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2)	93:176	EDEM2 (Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2) is one of the key-proteins suggested to be involved in the selection and degradation of misfolded proteins from the endoplasmic reticulum.
34712749	1	18	theme	Degradation-Enhancing	122:142	arg1	protein					167:173	Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2	100:175	Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2	100:175	EDEM2 (Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2) is one of the key-proteins suggested to be involved in the selection and degradation of misfolded proteins from the endoplasmic reticulum.
34712749	7	19	theme	proteins	1436:1443	arg1	proteins					1436:1443	proteins	1436:1443	proteins from the secretory pathway	1436:1470	This allowed us to map the fraction of glycoproteins with non-matured N-glycans from A375 melanoma cells and find or validate N-glycosylation sites of proteins from the secretory pathway.
34712749	7	19	theme	proteins	1436:1443	arg1	sites					1427:1431	N-glycosylation sites	1411:1431	N-glycosylation sites of proteins from the secretory pathway	1411:1470	This allowed us to map the fraction of glycoproteins with non-matured N-glycans from A375 melanoma cells and find or validate N-glycosylation sites of proteins from the secretory pathway.
34712749	1	20	from	selection	237:245	arg1	reticulum					306:314	the endoplasmic reticulum	290:314	the endoplasmic reticulum	290:314	EDEM2 (Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2) is one of the key-proteins suggested to be involved in the selection and degradation of misfolded proteins from the endoplasmic reticulum.
34712749	1	21	theme	alpha-Mannosidase-like	144:165	arg1	EDEM2					93:97	EDEM2	93:97	EDEM2 (Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2)	93:176	EDEM2 (Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2) is one of the key-proteins suggested to be involved in the selection and degradation of misfolded proteins from the endoplasmic reticulum.
34712749	1	21	theme	alpha-Mannosidase-like	144:165	arg1	protein					167:173	Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2	100:175	Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2	100:175	EDEM2 (Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2) is one of the key-proteins suggested to be involved in the selection and degradation of misfolded proteins from the endoplasmic reticulum.
34712749	8	22	theme	endogenous	1562:1571	arg1	EDEM2					1573:1577	endogenous EDEM2	1562:1577	endogenous EDEM2	1562:1577	The same dataset was also used to define glycoproteins altered by the down-regulation of endogenous EDEM2, which should contain its candidate-substrates.
34712749	11	23	theme	Cellular	1996:2003	arg1	[1					2016:2017	Molecular and Cellular Proteomics [1]	1982:2018	Molecular and Cellular Proteomics [1]	1982:2018	All the data described are associated with a research article published in Molecular and Cellular Proteomics [1].
34712749	0	24	theme	affinity	50:57	arg1	proteomics					59:68	affinity proteomics	50:68	affinity proteomics	50:68	Dataset of human EDEM2 melanoma cells proteomics, affinity proteomics and deglycoproteomics.
34712749	2	25	theme	Cell	520:523	arg1	Culture					525:531	Cell Culture	520:531	Cell Culture	520:531	The datasets discussed in this article are related to experiments covering affinity proteomics, label-free quantitative proteomics, deglycoproteomics and SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data of A375 melanoma cells with modified expression of EDEM2.
34712749	2	26	theme	A375	553:556	arg1	cells					567:571	A375 melanoma cells	553:571	A375 melanoma cells	553:571	The datasets discussed in this article are related to experiments covering affinity proteomics, label-free quantitative proteomics, deglycoproteomics and SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data of A375 melanoma cells with modified expression of EDEM2.
34712749	9	27	theme	second	1632:1637	arg1	step					1639:1642	a second step	1630:1642	a second step	1630:1642	In a second step we delineate the degradation kinetics of some of these proteins using a pulse SILAC strategy (pSILAC) thus complementing our initial findings with a fourth dataset.
34712749	7	28	from	sites	1427:1431	arg1	pathway					1464:1470	the secretory pathway	1450:1470	the secretory pathway	1450:1470	This allowed us to map the fraction of glycoproteins with non-matured N-glycans from A375 melanoma cells and find or validate N-glycosylation sites of proteins from the secretory pathway.
34712749	4	29	theme	SDF	823:825	arg1	experiments					872:882	SDF (Sucrose Density Fractionation)-nanoLC-MS/MS experiments	823:882	SDF (Sucrose Density Fractionation)-nanoLC-MS/MS experiments	823:882	The dataset was substantiated by SDF (Sucrose Density Fractionation)-nanoLC-MS/MS experiments, in an integrated workflow to validate EDEM2 identified partners and corroborate these with previous data.
34712749	2	30	theme	quantitative	424:435	arg1	proteomics					401:410	affinity proteomics	392:410	affinity proteomics	392:410	The datasets discussed in this article are related to experiments covering affinity proteomics, label-free quantitative proteomics, deglycoproteomics and SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data of A375 melanoma cells with modified expression of EDEM2.
34712749	2	30	theme	quantitative	424:435	arg1	proteomics					437:446	label-free quantitative proteomics	413:446	label-free quantitative proteomics	413:446	The datasets discussed in this article are related to experiments covering affinity proteomics, label-free quantitative proteomics, deglycoproteomics and SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data of A375 melanoma cells with modified expression of EDEM2.
34712749	2	31	dep	SILAC	471:475	arg1	Labeling					493:500	Stable Isotope Labeling	478:500	Stable Isotope Labeling by Amino Acids in Cell Culture	478:531	The datasets discussed in this article are related to experiments covering affinity proteomics, label-free quantitative proteomics, deglycoproteomics and SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data of A375 melanoma cells with modified expression of EDEM2.
34712749	2	32	with	data	545:548	arg1	expression					587:596	modified expression	578:596	modified expression of EDEM2	578:605	The datasets discussed in this article are related to experiments covering affinity proteomics, label-free quantitative proteomics, deglycoproteomics and SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data of A375 melanoma cells with modified expression of EDEM2.
34712749	5	33	theme	EDEM2	1029:1033	arg1	candidates					1045:1054	novel EDEM2 substrate candidates	1023:1054	novel EDEM2 substrate candidates using a two-step strategy	1023:1080	Our second aim was to delineate novel EDEM2 substrate candidates using a two-step strategy.
34712749	7	34	theme	N-glycosylation	1411:1425	arg1	proteins					1436:1443	proteins	1436:1443	proteins from the secretory pathway	1436:1470	This allowed us to map the fraction of glycoproteins with non-matured N-glycans from A375 melanoma cells and find or validate N-glycosylation sites of proteins from the secretory pathway.
34712749	7	34	theme	N-glycosylation	1411:1425	arg1	sites					1427:1431	N-glycosylation sites	1411:1431	N-glycosylation sites of proteins from the secretory pathway	1411:1470	This allowed us to map the fraction of glycoproteins with non-matured N-glycans from A375 melanoma cells and find or validate N-glycosylation sites of proteins from the secretory pathway.
34712749	2	35	theme	Isotope	485:491	arg1	Labeling					493:500	Stable Isotope Labeling	478:500	Stable Isotope Labeling by Amino Acids in Cell Culture	478:531	The datasets discussed in this article are related to experiments covering affinity proteomics, label-free quantitative proteomics, deglycoproteomics and SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data of A375 melanoma cells with modified expression of EDEM2.
34712749	11	36	theme	Molecular	1982:1990	arg1	[1					2016:2017	Molecular and Cellular Proteomics [1]	1982:2018	Molecular and Cellular Proteomics [1]	1982:2018	All the data described are associated with a research article published in Molecular and Cellular Proteomics [1].
34712749	5	37	theme	second	995:1000	arg1	aim					1002:1004	Our second aim	991:1004	Our second aim	991:1004	Our second aim was to delineate novel EDEM2 substrate candidates using a two-step strategy.
34712749	8	38	theme	same	1477:1480	arg1	dataset					1482:1488	The same dataset	1473:1488	The same dataset	1473:1488	The same dataset was also used to define glycoproteins altered by the down-regulation of endogenous EDEM2, which should contain its candidate-substrates.
34712749	0	39	theme	human	11:15	arg1	cells					32:36	human EDEM2 melanoma cells	11:36	human EDEM2 melanoma cells	11:36	Dataset of human EDEM2 melanoma cells proteomics, affinity proteomics and deglycoproteomics.
34712749	1	40	theme	Endoplasmic	100:110	arg1	reticulum					112:120	Endoplasmic reticulum	100:120	Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2	100:175	EDEM2 (Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2) is one of the key-proteins suggested to be involved in the selection and degradation of misfolded proteins from the endoplasmic reticulum.
34712749	7	41	from	pathway	1464:1470	arg1	proteins					1436:1443	proteins	1436:1443	proteins from the secretory pathway	1436:1470	This allowed us to map the fraction of glycoproteins with non-matured N-glycans from A375 melanoma cells and find or validate N-glycosylation sites of proteins from the secretory pathway.
34712749	7	41	from	pathway	1464:1470	arg1	sites					1427:1431	N-glycosylation sites	1411:1431	N-glycosylation sites of proteins from the secretory pathway	1411:1470	This allowed us to map the fraction of glycoproteins with non-matured N-glycans from A375 melanoma cells and find or validate N-glycosylation sites of proteins from the secretory pathway.
34712749	4	42	theme	-nanoLC-MS/MS	858:870	arg1	experiments					872:882	SDF (Sucrose Density Fractionation)-nanoLC-MS/MS experiments	823:882	SDF (Sucrose Density Fractionation)-nanoLC-MS/MS experiments	823:882	The dataset was substantiated by SDF (Sucrose Density Fractionation)-nanoLC-MS/MS experiments, in an integrated workflow to validate EDEM2 identified partners and corroborate these with previous data.
34712749	0	43	theme	melanoma	23:30	arg1	cells					32:36	human EDEM2 melanoma cells	11:36	human EDEM2 melanoma cells	11:36	Dataset of human EDEM2 melanoma cells proteomics, affinity proteomics and deglycoproteomics.
34712749	0	44	dep	proteomics	38:47	arg1	Dataset					0:6	Dataset	0:6	Dataset of human EDEM2 melanoma cells	0:36	Dataset of human EDEM2 melanoma cells proteomics, affinity proteomics and deglycoproteomics.
34712749	7	45	theme	melanoma	1375:1382	arg1	cells					1384:1388	A375 melanoma cells	1370:1388	A375 melanoma cells	1370:1388	This allowed us to map the fraction of glycoproteins with non-matured N-glycans from A375 melanoma cells and find or validate N-glycosylation sites of proteins from the secretory pathway.
34712749	3	46	theme	potential	665:673	arg1	partners					687:694	its potential interaction partners	661:694	its potential interaction partners	661:694	Our first aim was to affinity-enrich EDEM2 alongside its potential interaction partners and analyse the obtained samples by nanoLC-MS/MS to identify novel EDEM2 associated proteins.
34712749	7	47	from	cells	1384:1388	arg1	N-glycans					1355:1363	non-matured N-glycans	1343:1363	non-matured N-glycans from A375 melanoma cells	1343:1388	This allowed us to map the fraction of glycoproteins with non-matured N-glycans from A375 melanoma cells and find or validate N-glycosylation sites of proteins from the secretory pathway.
34712749	3	48	theme	obtained	712:719	arg1	samples					721:727	the obtained samples	708:727	the obtained samples by nanoLC-MS/MS to identify novel EDEM2 associated proteins	708:787	Our first aim was to affinity-enrich EDEM2 alongside its potential interaction partners and analyse the obtained samples by nanoLC-MS/MS to identify novel EDEM2 associated proteins.
34712749	4	49	theme	Sucrose	828:834	arg1	Fractionation					844:856	Sucrose Density Fractionation	828:856	Sucrose Density Fractionation	828:856	The dataset was substantiated by SDF (Sucrose Density Fractionation)-nanoLC-MS/MS experiments, in an integrated workflow to validate EDEM2 identified partners and corroborate these with previous data.
34712749	4	49	theme	Sucrose	828:834	arg1	SDF					823:825	SDF	823:825	SDF (Sucrose Density Fractionation)-nanoLC-MS/MS experiments	823:882	The dataset was substantiated by SDF (Sucrose Density Fractionation)-nanoLC-MS/MS experiments, in an integrated workflow to validate EDEM2 identified partners and corroborate these with previous data.
34712749	9	50	theme	some	1685:1688	arg1	kinetics					1673:1680	the degradation kinetics	1657:1680	the degradation kinetics of some of these proteins	1657:1706	In a second step we delineate the degradation kinetics of some of these proteins using a pulse SILAC strategy (pSILAC) thus complementing our initial findings with a fourth dataset.
34712749	7	51	theme	non-matured	1343:1353	arg1	N-glycans					1355:1363	non-matured N-glycans	1343:1363	non-matured N-glycans from A375 melanoma cells	1343:1388	This allowed us to map the fraction of glycoproteins with non-matured N-glycans from A375 melanoma cells and find or validate N-glycosylation sites of proteins from the secretory pathway.
34712749	6	52	dep	enriched	1191:1198	arg1	A					1189:1189	Concanavalin A	1176:1189	Concanavalin A	1176:1189	The first one refers to the deglycoproteomics dataset, which covers nanoLC-MS/MS analysis of Concanavalin A enriched glycopeptides released by endoglycosidase digestion from A375 melanoma cell lysates.
34712749	2	53	theme	affinity	392:399	arg1	data					545:548	SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data	471:548	SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data	471:548	The datasets discussed in this article are related to experiments covering affinity proteomics, label-free quantitative proteomics, deglycoproteomics and SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data of A375 melanoma cells with modified expression of EDEM2.
34712749	2	53	theme	affinity	392:399	arg1	proteomics					401:410	affinity proteomics	392:410	affinity proteomics	392:410	The datasets discussed in this article are related to experiments covering affinity proteomics, label-free quantitative proteomics, deglycoproteomics and SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data of A375 melanoma cells with modified expression of EDEM2.
34712749	2	53	theme	affinity	392:399	arg1	deglycoproteomics					449:465	deglycoproteomics	449:465	deglycoproteomics	449:465	The datasets discussed in this article are related to experiments covering affinity proteomics, label-free quantitative proteomics, deglycoproteomics and SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data of A375 melanoma cells with modified expression of EDEM2.
34712749	2	53	theme	affinity	392:399	arg1	proteomics					437:446	label-free quantitative proteomics	413:446	label-free quantitative proteomics	413:446	The datasets discussed in this article are related to experiments covering affinity proteomics, label-free quantitative proteomics, deglycoproteomics and SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data of A375 melanoma cells with modified expression of EDEM2.
34712749	9	54	theme	pulse	1716:1720	arg1	pSILAC					1738:1743	pSILAC	1738:1743	pSILAC	1738:1743	In a second step we delineate the degradation kinetics of some of these proteins using a pulse SILAC strategy (pSILAC) thus complementing our initial findings with a fourth dataset.
34712749	9	54	theme	pulse	1716:1720	arg1	strategy					1728:1735	a pulse SILAC strategy	1714:1735	a pulse SILAC strategy (pSILAC)	1714:1744	In a second step we delineate the degradation kinetics of some of these proteins using a pulse SILAC strategy (pSILAC) thus complementing our initial findings with a fourth dataset.
34712749	7	55	theme	glycoproteins	1324:1336	arg1	fraction					1312:1319	the fraction	1308:1319	the fraction of glycoproteins with non-matured N-glycans from A375 melanoma cells	1308:1388	This allowed us to map the fraction of glycoproteins with non-matured N-glycans from A375 melanoma cells and find or validate N-glycosylation sites of proteins from the secretory pathway.
34712749	11	56	theme	research	1952:1959	arg1	article					1961:1967	a research article	1950:1967	a research article published in Molecular and Cellular Proteomics [1]	1950:2018	All the data described are associated with a research article published in Molecular and Cellular Proteomics [1].
34712749	1	57	theme	proteins	276:283	arg1	degradation					251:261	degradation	251:261	degradation	251:261	EDEM2 (Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2) is one of the key-proteins suggested to be involved in the selection and degradation of misfolded proteins from the endoplasmic reticulum.
34712749	1	57	theme	proteins	276:283	arg1	selection					237:245	selection	237:245	selection	237:245	EDEM2 (Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2) is one of the key-proteins suggested to be involved in the selection and degradation of misfolded proteins from the endoplasmic reticulum.
34712749	6	58	theme	A375	1257:1260	arg1	lysates					1276:1282	A375 melanoma cell lysates	1257:1282	A375 melanoma cell lysates	1257:1282	The first one refers to the deglycoproteomics dataset, which covers nanoLC-MS/MS analysis of Concanavalin A enriched glycopeptides released by endoglycosidase digestion from A375 melanoma cell lysates.
34712749	3	59	theme	associated	769:778	arg1	proteins					780:787	novel EDEM2 associated proteins	757:787	novel EDEM2 associated proteins	757:787	Our first aim was to affinity-enrich EDEM2 alongside its potential interaction partners and analyse the obtained samples by nanoLC-MS/MS to identify novel EDEM2 associated proteins.
34712749	1	60	from	reticulum	306:314	arg1	degradation					251:261	degradation	251:261	degradation	251:261	EDEM2 (Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2) is one of the key-proteins suggested to be involved in the selection and degradation of misfolded proteins from the endoplasmic reticulum.
34712749	1	60	from	reticulum	306:314	arg1	proteins					276:283	misfolded proteins	266:283	misfolded proteins from the endoplasmic reticulum	266:314	EDEM2 (Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2) is one of the key-proteins suggested to be involved in the selection and degradation of misfolded proteins from the endoplasmic reticulum.
34712749	1	60	from	reticulum	306:314	arg1	selection					237:245	selection	237:245	selection	237:245	EDEM2 (Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2) is one of the key-proteins suggested to be involved in the selection and degradation of misfolded proteins from the endoplasmic reticulum.
34712749	10	61	theme	analysis	1829:1836	arg1	findings					1842:1849	Beside nanoLC-MS/MS analysis our findings	1809:1849	Beside nanoLC-MS/MS analysis our findings	1809:1849	Beside nanoLC-MS/MS analysis our findings were also validated by various biochemical experiments.
34712749	8	62	gly	glycoproteins	1514:1526	arg1	glycoproteins					1514:1526	glycoproteins	1514:1526	glycoproteins altered by the down-regulation of endogenous EDEM2	1514:1577	The same dataset was also used to define glycoproteins altered by the down-regulation of endogenous EDEM2, which should contain its candidate-substrates.
34712749	3	63	theme	novel	757:761	arg1	proteins					780:787	novel EDEM2 associated proteins	757:787	novel EDEM2 associated proteins	757:787	Our first aim was to affinity-enrich EDEM2 alongside its potential interaction partners and analyse the obtained samples by nanoLC-MS/MS to identify novel EDEM2 associated proteins.
34712749	1	64	theme	key-proteins	192:203	arg1	key-proteins					192:203	the key-proteins	188:203	the key-proteins suggested to be involved in the selection and degradation of misfolded proteins from the endoplasmic reticulum	188:314	EDEM2 (Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2) is one of the key-proteins suggested to be involved in the selection and degradation of misfolded proteins from the endoplasmic reticulum.
34712749	1	64	theme	key-proteins	192:203	arg1	one					181:183	one	181:183	one	181:183	EDEM2 (Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2) is one of the key-proteins suggested to be involved in the selection and degradation of misfolded proteins from the endoplasmic reticulum.
34712749	4	65	theme	integrated	891:900	arg1	workflow					902:909	an integrated workflow	888:909	an integrated workflow to validate EDEM2 identified partners and corroborate these with previous data	888:988	The dataset was substantiated by SDF (Sucrose Density Fractionation)-nanoLC-MS/MS experiments, in an integrated workflow to validate EDEM2 identified partners and corroborate these with previous data.
34712749	6	66	gly	glycopeptides	1200:1212	arg2	glycopeptides					1200:1212	Concanavalin A enriched glycopeptides	1176:1212	Concanavalin A enriched glycopeptides released by endoglycosidase digestion from A375 melanoma cell lysates	1176:1282	The first one refers to the deglycoproteomics dataset, which covers nanoLC-MS/MS analysis of Concanavalin A enriched glycopeptides released by endoglycosidase digestion from A375 melanoma cell lysates.
34712749	6	67	theme	glycopeptides	1200:1212	arg1	analysis					1164:1171	nanoLC-MS/MS analysis	1151:1171	nanoLC-MS/MS analysis of Concanavalin A enriched glycopeptides released by endoglycosidase digestion from A375 melanoma cell lysates	1151:1282	The first one refers to the deglycoproteomics dataset, which covers nanoLC-MS/MS analysis of Concanavalin A enriched glycopeptides released by endoglycosidase digestion from A375 melanoma cell lysates.
34712749	2	68	theme	melanoma	558:565	arg1	cells					567:571	A375 melanoma cells	553:571	A375 melanoma cells	553:571	The datasets discussed in this article are related to experiments covering affinity proteomics, label-free quantitative proteomics, deglycoproteomics and SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data of A375 melanoma cells with modified expression of EDEM2.
34712749	10	69	theme	biochemical	1882:1892	arg1	experiments					1894:1904	various biochemical experiments	1874:1904	various biochemical experiments	1874:1904	Beside nanoLC-MS/MS analysis our findings were also validated by various biochemical experiments.
34712749	1	70	theme	reticulum	112:120	arg1	EDEM2					93:97	EDEM2	93:97	EDEM2 (Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2)	93:176	EDEM2 (Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2) is one of the key-proteins suggested to be involved in the selection and degradation of misfolded proteins from the endoplasmic reticulum.
34712749	1	70	theme	reticulum	112:120	arg1	protein					167:173	Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2	100:175	Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2	100:175	EDEM2 (Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2) is one of the key-proteins suggested to be involved in the selection and degradation of misfolded proteins from the endoplasmic reticulum.
34712749	2	71	theme	EDEM2	601:605	arg1	expression					587:596	modified expression	578:596	modified expression of EDEM2	578:605	The datasets discussed in this article are related to experiments covering affinity proteomics, label-free quantitative proteomics, deglycoproteomics and SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data of A375 melanoma cells with modified expression of EDEM2.
34712749	7	72	theme	secretory	1454:1462	arg1	pathway					1464:1470	the secretory pathway	1450:1470	the secretory pathway	1450:1470	This allowed us to map the fraction of glycoproteins with non-matured N-glycans from A375 melanoma cells and find or validate N-glycosylation sites of proteins from the secretory pathway.
34712749	0	73	theme	cells	32:36	arg1	Dataset					0:6	Dataset	0:6	Dataset of human EDEM2 melanoma cells	0:36	Dataset of human EDEM2 melanoma cells proteomics, affinity proteomics and deglycoproteomics.
34712749	8	74	theme	EDEM2	1573:1577	arg1	down-regulation					1543:1557	the down-regulation	1539:1557	the down-regulation of endogenous EDEM2	1539:1577	The same dataset was also used to define glycoproteins altered by the down-regulation of endogenous EDEM2, which should contain its candidate-substrates.
34712749	6	75	theme	cell	1271:1274	arg1	lysates					1276:1282	A375 melanoma cell lysates	1257:1282	A375 melanoma cell lysates	1257:1282	The first one refers to the deglycoproteomics dataset, which covers nanoLC-MS/MS analysis of Concanavalin A enriched glycopeptides released by endoglycosidase digestion from A375 melanoma cell lysates.
34712749	6	76	attach	released	1214:1221	arg2	glycopeptides					1200:1212	Concanavalin A enriched glycopeptides	1176:1212	Concanavalin A enriched glycopeptides released by endoglycosidase digestion from A375 melanoma cell lysates	1176:1282	The first one refers to the deglycoproteomics dataset, which covers nanoLC-MS/MS analysis of Concanavalin A enriched glycopeptides released by endoglycosidase digestion from A375 melanoma cell lysates.
34712749	6	76	attach	released	1214:1221	arg1	lysates					1276:1282	A375 melanoma cell lysates	1257:1282	A375 melanoma cell lysates	1257:1282	The first one refers to the deglycoproteomics dataset, which covers nanoLC-MS/MS analysis of Concanavalin A enriched glycopeptides released by endoglycosidase digestion from A375 melanoma cell lysates.
34712749	4	77	theme	previous	976:983	arg1	data					985:988	previous data	976:988	previous data	976:988	The dataset was substantiated by SDF (Sucrose Density Fractionation)-nanoLC-MS/MS experiments, in an integrated workflow to validate EDEM2 identified partners and corroborate these with previous data.
34712749	7	78	gly	N-glycosylation	1411:1425	arg1	proteins					1436:1443	proteins	1436:1443	proteins from the secretory pathway	1436:1470	This allowed us to map the fraction of glycoproteins with non-matured N-glycans from A375 melanoma cells and find or validate N-glycosylation sites of proteins from the secretory pathway.
34712749	7	78	gly	N-glycosylation	1411:1425	arg2	sites					1427:1431	N-glycosylation sites	1411:1431	N-glycosylation sites of proteins from the secretory pathway	1411:1470	This allowed us to map the fraction of glycoproteins with non-matured N-glycans from A375 melanoma cells and find or validate N-glycosylation sites of proteins from the secretory pathway.
34712749	7	78	gly	N-glycosylation	1411:1425	arg2	pathway					1464:1470	the secretory pathway	1450:1470	the secretory pathway	1450:1470	This allowed us to map the fraction of glycoproteins with non-matured N-glycans from A375 melanoma cells and find or validate N-glycosylation sites of proteins from the secretory pathway.
34712749	7	78	gly	N-glycosylation	1411:1425	arg2	proteins					1436:1443	proteins	1436:1443	proteins from the secretory pathway	1436:1470	This allowed us to map the fraction of glycoproteins with non-matured N-glycans from A375 melanoma cells and find or validate N-glycosylation sites of proteins from the secretory pathway.
34712749	5	79	theme	novel	1023:1027	arg1	candidates					1045:1054	novel EDEM2 substrate candidates	1023:1054	novel EDEM2 substrate candidates using a two-step strategy	1023:1080	Our second aim was to delineate novel EDEM2 substrate candidates using a two-step strategy.
34712749	2	80	theme	proteomics	534:543	arg1	data					545:548	SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data	471:548	SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data	471:548	The datasets discussed in this article are related to experiments covering affinity proteomics, label-free quantitative proteomics, deglycoproteomics and SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data of A375 melanoma cells with modified expression of EDEM2.
34712749	2	80	theme	proteomics	534:543	arg1	proteomics					401:410	affinity proteomics	392:410	affinity proteomics	392:410	The datasets discussed in this article are related to experiments covering affinity proteomics, label-free quantitative proteomics, deglycoproteomics and SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data of A375 melanoma cells with modified expression of EDEM2.
34712749	2	81	from	Acids	511:515	arg1	Culture					525:531	Cell Culture	520:531	Cell Culture	520:531	The datasets discussed in this article are related to experiments covering affinity proteomics, label-free quantitative proteomics, deglycoproteomics and SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data of A375 melanoma cells with modified expression of EDEM2.
34712749	2	82	theme	label-free	413:422	arg1	proteomics					401:410	affinity proteomics	392:410	affinity proteomics	392:410	The datasets discussed in this article are related to experiments covering affinity proteomics, label-free quantitative proteomics, deglycoproteomics and SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data of A375 melanoma cells with modified expression of EDEM2.
34712749	2	82	theme	label-free	413:422	arg1	proteomics					437:446	label-free quantitative proteomics	413:446	label-free quantitative proteomics	413:446	The datasets discussed in this article are related to experiments covering affinity proteomics, label-free quantitative proteomics, deglycoproteomics and SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data of A375 melanoma cells with modified expression of EDEM2.
34712749	6	83	theme	deglycoproteomics	1111:1127	arg1	dataset					1129:1135	the deglycoproteomics dataset	1107:1135	the deglycoproteomics dataset	1107:1135	The first one refers to the deglycoproteomics dataset, which covers nanoLC-MS/MS analysis of Concanavalin A enriched glycopeptides released by endoglycosidase digestion from A375 melanoma cell lysates.
34712749	2	84	theme	Stable	478:483	arg1	Labeling					493:500	Stable Isotope Labeling	478:500	Stable Isotope Labeling by Amino Acids in Cell Culture	478:531	The datasets discussed in this article are related to experiments covering affinity proteomics, label-free quantitative proteomics, deglycoproteomics and SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data of A375 melanoma cells with modified expression of EDEM2.
34712749	0	85	theme	EDEM2	17:21	arg1	cells					32:36	human EDEM2 melanoma cells	11:36	human EDEM2 melanoma cells	11:36	Dataset of human EDEM2 melanoma cells proteomics, affinity proteomics and deglycoproteomics.
34712749	1	86	theme	endoplasmic	294:304	arg1	reticulum					306:314	the endoplasmic reticulum	290:314	the endoplasmic reticulum	290:314	EDEM2 (Endoplasmic reticulum Degradation-Enhancing alpha-Mannosidase-like protein 2) is one of the key-proteins suggested to be involved in the selection and degradation of misfolded proteins from the endoplasmic reticulum.
34712749	7	87	gly	glycoproteins	1324:1336	arg1	glycoproteins					1324:1336	glycoproteins	1324:1336	glycoproteins with non-matured N-glycans from A375 melanoma cells	1324:1388	This allowed us to map the fraction of glycoproteins with non-matured N-glycans from A375 melanoma cells and find or validate N-glycosylation sites of proteins from the secretory pathway.
34712749	9	88	theme	degradation	1661:1671	arg1	kinetics					1673:1680	the degradation kinetics	1657:1680	the degradation kinetics of some of these proteins	1657:1706	In a second step we delineate the degradation kinetics of some of these proteins using a pulse SILAC strategy (pSILAC) thus complementing our initial findings with a fourth dataset.
34712749	2	89	theme	SILAC	471:475	arg1	data					545:548	SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data	471:548	SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data	471:548	The datasets discussed in this article are related to experiments covering affinity proteomics, label-free quantitative proteomics, deglycoproteomics and SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data of A375 melanoma cells with modified expression of EDEM2.
34712749	2	89	theme	SILAC	471:475	arg1	proteomics					401:410	affinity proteomics	392:410	affinity proteomics	392:410	The datasets discussed in this article are related to experiments covering affinity proteomics, label-free quantitative proteomics, deglycoproteomics and SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data of A375 melanoma cells with modified expression of EDEM2.
34712749	3	90	theme	interaction	675:685	arg1	partners					687:694	its potential interaction partners	661:694	its potential interaction partners	661:694	Our first aim was to affinity-enrich EDEM2 alongside its potential interaction partners and analyse the obtained samples by nanoLC-MS/MS to identify novel EDEM2 associated proteins.
34712749	10	91	theme	Beside	1809:1814	arg1	findings					1842:1849	Beside nanoLC-MS/MS analysis our findings	1809:1849	Beside nanoLC-MS/MS analysis our findings	1809:1849	Beside nanoLC-MS/MS analysis our findings were also validated by various biochemical experiments.
34712749	4	92	dep	identified	929:938	arg1	EDEM2					923:927	EDEM2	923:927	EDEM2	923:927	The dataset was substantiated by SDF (Sucrose Density Fractionation)-nanoLC-MS/MS experiments, in an integrated workflow to validate EDEM2 identified partners and corroborate these with previous data.
34712749	5	93	theme	two-step	1064:1071	arg1	strategy					1073:1080	a two-step strategy	1062:1080	a two-step strategy	1062:1080	Our second aim was to delineate novel EDEM2 substrate candidates using a two-step strategy.
34712749	7	94	theme	A375	1370:1373	arg1	cells					1384:1388	A375 melanoma cells	1370:1388	A375 melanoma cells	1370:1388	This allowed us to map the fraction of glycoproteins with non-matured N-glycans from A375 melanoma cells and find or validate N-glycosylation sites of proteins from the secretory pathway.
34712749	2	95	theme	Amino	505:509	arg1	Acids					511:515	Amino Acids	505:515	Amino Acids in Cell Culture	505:531	The datasets discussed in this article are related to experiments covering affinity proteomics, label-free quantitative proteomics, deglycoproteomics and SILAC (Stable Isotope Labeling by Amino Acids in Cell Culture) proteomics data of A375 melanoma cells with modified expression of EDEM2.
34712749	6	96	theme	enriched	1191:1198	arg1	glycopeptides					1200:1212	Concanavalin A enriched glycopeptides	1176:1212	Concanavalin A enriched glycopeptides released by endoglycosidase digestion from A375 melanoma cell lysates	1176:1282	The first one refers to the deglycoproteomics dataset, which covers nanoLC-MS/MS analysis of Concanavalin A enriched glycopeptides released by endoglycosidase digestion from A375 melanoma cell lysates.
34712749	9	97	theme	initial	1769:1775	arg1	findings					1777:1784	our initial findings	1765:1784	our initial findings	1765:1784	In a second step we delineate the degradation kinetics of some of these proteins using a pulse SILAC strategy (pSILAC) thus complementing our initial findings with a fourth dataset.
34712749	5	98	theme	substrate	1035:1043	arg1	candidates					1045:1054	novel EDEM2 substrate candidates	1023:1054	novel EDEM2 substrate candidates using a two-step strategy	1023:1080	Our second aim was to delineate novel EDEM2 substrate candidates using a two-step strategy.
34712749	6	99	theme	Concanavalin	1176:1187	arg1	A					1189:1189	Concanavalin A	1176:1189	Concanavalin A	1176:1189	The first one refers to the deglycoproteomics dataset, which covers nanoLC-MS/MS analysis of Concanavalin A enriched glycopeptides released by endoglycosidase digestion from A375 melanoma cell lysates.
34712749	6	100	theme	nanoLC-MS/MS	1151:1162	arg1	analysis					1164:1171	nanoLC-MS/MS analysis	1151:1171	nanoLC-MS/MS analysis of Concanavalin A enriched glycopeptides released by endoglycosidase digestion from A375 melanoma cell lysates	1151:1282	The first one refers to the deglycoproteomics dataset, which covers nanoLC-MS/MS analysis of Concanavalin A enriched glycopeptides released by endoglycosidase digestion from A375 melanoma cell lysates.
34712749	11	101	theme	Proteomics	2005:2014	arg1	[1					2016:2017	Molecular and Cellular Proteomics [1]	1982:2018	Molecular and Cellular Proteomics [1]	1982:2018	All the data described are associated with a research article published in Molecular and Cellular Proteomics [1].
34712749	4	102	theme	Density	836:842	arg1	Fractionation					844:856	Sucrose Density Fractionation	828:856	Sucrose Density Fractionation	828:856	The dataset was substantiated by SDF (Sucrose Density Fractionation)-nanoLC-MS/MS experiments, in an integrated workflow to validate EDEM2 identified partners and corroborate these with previous data.
34712749	4	102	theme	Density	836:842	arg1	SDF					823:825	SDF	823:825	SDF (Sucrose Density Fractionation)-nanoLC-MS/MS experiments	823:882	The dataset was substantiated by SDF (Sucrose Density Fractionation)-nanoLC-MS/MS experiments, in an integrated workflow to validate EDEM2 identified partners and corroborate these with previous data.
32250240	3	0	theme	taxonomic	183:191	arg1	position					193:200	The taxonomic position	179:200	The taxonomic position of the strain	179:214	The taxonomic position of the strain was investigated by using a polyphasic approach.
32250240	4	1	theme	CFH	281:283	arg1	S0261T					285:290	strain CFH S0261T	274:290	strain CFH S0261T	274:290	Cells of strain CFH S0261T were Gram-reaction-positive, aerobic, non-motile.
32250240	9	2	theme	DDH	1012:1014	arg1	%					1023:1023	ANIb <90.61 % and DDH <53.40 %	994:1023	ANIb <90.61 % and DDH <53.40 %	994:1023	The ANI values and in silico DDH values between CFH S0261T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %).
32250240	9	2	theme	DDH	1012:1014	arg1	values					872:877	The ANI values	864:877	The ANI values	864:877	The ANI values and in silico DDH values between CFH S0261T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %).
32250240	9	2	theme	DDH	1012:1014	arg1	low					989:991	low	989:991	low	989:991	The ANI values and in silico DDH values between CFH S0261T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %).
32250240	7	3	theme	22139T	780:785	arg1	strains					647:653	the type strains	638:653	the type strains of Amycolatopsis niigatensis LC11T (98.95 %), Amycolatopsis echigonensis LC2T (98.81 %) and Amycolatopsis albidoflavus IMSNU 22139T (98.73 %)	638:795	The 16S rRNA gene sequence similarity indicated that strain CFH S0261T is most closely related to the type strains of Amycolatopsis niigatensis LC11T (98.95 %), Amycolatopsis echigonensis LC2T (98.81 %) and Amycolatopsis albidoflavus IMSNU 22139T (98.73 %).
32250240	10	4	theme	cell	1031:1034	arg1	acid					1120:1123	meso-diaminopimelic acid	1100:1123	meso-diaminopimelic acid	1100:1123	The cell wall diamino acid in the peptidoglycan of strain CFH S0261T was meso-diaminopimelic acid and the whole-cell hydrolysate comprised arabinose, galactose, glucose, rhamnose and ribose.
32250240	10	4	theme	cell	1031:1034	arg1	acid					1049:1052	The cell wall diamino acid	1027:1052	The cell wall diamino acid in the peptidoglycan of strain CFH S0261T	1027:1094	The cell wall diamino acid in the peptidoglycan of strain CFH S0261T was meso-diaminopimelic acid and the whole-cell hydrolysate comprised arabinose, galactose, glucose, rhamnose and ribose.
32250240	12	5	theme	cellular	1270:1277	arg1	C16 					1296:1299	C16 	1296:1299	C16 	1296:1299	The major cellular fatty acids were C16 : 0, iso-C15 : 0 and iso-C16 : 0.
32250240	12	5	theme	cellular	1270:1277	arg1	acids					1285:1289	The major cellular fatty acids	1260:1289	The major cellular fatty acids	1260:1289	The major cellular fatty acids were C16 : 0, iso-C15 : 0 and iso-C16 : 0.
32250240	8	6	theme	69.5 mol	853:860	arg1	%					861:861	69.5 mol%	853:861	69.5 mol%	853:861	The whole-genome of CFH S0261T showed a G+C content of 69.5 mol%.
32250240	10	7	from	acid	1049:1052	arg1	peptidoglycan					1061:1073	the peptidoglycan	1057:1073	the peptidoglycan of strain CFH S0261T	1057:1094	The cell wall diamino acid in the peptidoglycan of strain CFH S0261T was meso-diaminopimelic acid and the whole-cell hydrolysate comprised arabinose, galactose, glucose, rhamnose and ribose.
32250240	6	8	theme	gene	453:456	arg1	sequence					458:465	the 16S rRNA gene sequence	440:465	the 16S rRNA gene sequence	440:465	Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CFH S0261T was a member of the genus Amycolatopsis.
32250240	7	9	theme	albidoflavus	761:772	arg1	22139T					780:785	Amycolatopsis albidoflavus IMSNU 22139T	747:785	Amycolatopsis albidoflavus IMSNU 22139T (98.73 %)	747:795	The 16S rRNA gene sequence similarity indicated that strain CFH S0261T is most closely related to the type strains of Amycolatopsis niigatensis LC11T (98.95 %), Amycolatopsis echigonensis LC2T (98.81 %) and Amycolatopsis albidoflavus IMSNU 22139T (98.73 %).
32250240	7	9	theme	albidoflavus	761:772	arg1	%					794:794	98.73 %	788:794	98.73 %	788:794	The 16S rRNA gene sequence similarity indicated that strain CFH S0261T is most closely related to the type strains of Amycolatopsis niigatensis LC11T (98.95 %), Amycolatopsis echigonensis LC2T (98.81 %) and Amycolatopsis albidoflavus IMSNU 22139T (98.73 %).
32250240	14	10	theme	genotypic	1557:1565	arg1	data					1584:1587	phenotypic, genotypic and phylogenetic data	1545:1587	phenotypic, genotypic and phylogenetic data	1545:1587	On the basis of phenotypic, genotypic and phylogenetic data, strain CFH S0261T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis nivea sp.
32250240	3	11	theme	strain	209:214	arg1	position					193:200	The taxonomic position	179:200	The taxonomic position of the strain	179:214	The taxonomic position of the strain was investigated by using a polyphasic approach.
32250240	6	12	theme	16S	444:446	arg1	rRNA					448:451	16S rRNA	444:451	the 16S rRNA gene sequence	440:465	Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CFH S0261T was a member of the genus Amycolatopsis.
32250240	10	13	theme	wall	1036:1039	arg1	acid					1120:1123	meso-diaminopimelic acid	1100:1123	meso-diaminopimelic acid	1100:1123	The cell wall diamino acid in the peptidoglycan of strain CFH S0261T was meso-diaminopimelic acid and the whole-cell hydrolysate comprised arabinose, galactose, glucose, rhamnose and ribose.
32250240	10	13	theme	wall	1036:1039	arg1	acid					1049:1052	The cell wall diamino acid	1027:1052	The cell wall diamino acid in the peptidoglycan of strain CFH S0261T	1027:1094	The cell wall diamino acid in the peptidoglycan of strain CFH S0261T was meso-diaminopimelic acid and the whole-cell hydrolysate comprised arabinose, galactose, glucose, rhamnose and ribose.
32250240	14	14	theme	Amycolatopsis	1648:1660	arg1	species					1627:1633	a novel species	1619:1633	a novel species	1619:1633	On the basis of phenotypic, genotypic and phylogenetic data, strain CFH S0261T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis nivea sp.
32250240	6	15	theme	genus	519:523	arg1	Amycolatopsis					525:537	the genus Amycolatopsis	515:537	the genus Amycolatopsis	515:537	Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CFH S0261T was a member of the genus Amycolatopsis.
32250240	14	16	theme	phylogenetic	1571:1582	arg1	data					1584:1587	phenotypic, genotypic and phylogenetic data	1545:1587	phenotypic, genotypic and phylogenetic data	1545:1587	On the basis of phenotypic, genotypic and phylogenetic data, strain CFH S0261T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis nivea sp.
32250240	16	17	theme	type	1728:1731	arg1	S0261T					1747:1752	CFH S0261T	1743:1752	CFH S0261T (=KCTC 39515T =CCTCC AA 2014028T)	1743:1786	The type strain is CFH S0261T (=KCTC 39515T =CCTCC AA 2014028T).
32250240	16	17	theme	type	1728:1731	arg1	strain					1733:1738	The type strain	1724:1738	The type strain	1724:1738	The type strain is CFH S0261T (=KCTC 39515T =CCTCC AA 2014028T).
32250240	14	18	theme	genus	1642:1646	arg1	Amycolatopsis					1648:1660	the genus Amycolatopsis	1638:1660	the genus Amycolatopsis	1638:1660	On the basis of phenotypic, genotypic and phylogenetic data, strain CFH S0261T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis nivea sp.
32250240	9	19	theme	in	883:884	arg1	values					897:902	in silico DDH values	883:902	in silico DDH values	883:902	The ANI values and in silico DDH values between CFH S0261T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %).
32250240	7	20	theme	CFH	600:602	arg1	S0261T					604:609	strain CFH S0261T	593:609	strain CFH S0261T	593:609	The 16S rRNA gene sequence similarity indicated that strain CFH S0261T is most closely related to the type strains of Amycolatopsis niigatensis LC11T (98.95 %), Amycolatopsis echigonensis LC2T (98.81 %) and Amycolatopsis albidoflavus IMSNU 22139T (98.73 %).
32250240	13	21	theme	polar	1338:1342	arg1	profile					1350:1356	The polar lipid profile	1334:1356	The polar lipid profile	1334:1356	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and four unidentified glycolipids.
32250240	14	22	theme	nivea	1696:1700	arg1	sp					1702:1703	the name Amycolatopsis nivea sp	1673:1703	the name Amycolatopsis nivea sp	1673:1703	On the basis of phenotypic, genotypic and phylogenetic data, strain CFH S0261T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis nivea sp.
32250240	3	23	theme	polyphasic	244:253	arg1	approach					255:262	a polyphasic approach	242:262	a polyphasic approach	242:262	The taxonomic position of the strain was investigated by using a polyphasic approach.
32250240	9	24	theme	DDH	893:895	arg1	values					897:902	in silico DDH values	883:902	in silico DDH values	883:902	The ANI values and in silico DDH values between CFH S0261T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %).
32250240	9	25	theme	Amycolatopsis	958:970	arg1	S0261T					916:921	CFH S0261T	912:921	CFH S0261T	912:921	The ANI values and in silico DDH values between CFH S0261T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %).
32250240	9	25	theme	Amycolatopsis	958:970	arg1	species					937:943	the other species	927:943	the other species of the genus Amycolatopsis	927:970	The ANI values and in silico DDH values between CFH S0261T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %).
32250240	10	26	theme	diamino	1041:1047	arg1	acid					1120:1123	meso-diaminopimelic acid	1100:1123	meso-diaminopimelic acid	1100:1123	The cell wall diamino acid in the peptidoglycan of strain CFH S0261T was meso-diaminopimelic acid and the whole-cell hydrolysate comprised arabinose, galactose, glucose, rhamnose and ribose.
32250240	10	26	theme	diamino	1041:1047	arg1	acid					1049:1052	The cell wall diamino acid	1027:1052	The cell wall diamino acid in the peptidoglycan of strain CFH S0261T	1027:1094	The cell wall diamino acid in the peptidoglycan of strain CFH S0261T was meso-diaminopimelic acid and the whole-cell hydrolysate comprised arabinose, galactose, glucose, rhamnose and ribose.
32250240	14	27	dep	data	1584:1587	arg1	the					1532:1534	the	1532:1534	the	1532:1534	On the basis of phenotypic, genotypic and phylogenetic data, strain CFH S0261T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis nivea sp.
32250240	14	27	dep	data	1584:1587	arg1	basis					1536:1540	basis	1536:1540	basis	1536:1540	On the basis of phenotypic, genotypic and phylogenetic data, strain CFH S0261T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis nivea sp.
32250240	10	28	theme	strain	1078:1083	arg1	S0261T					1089:1094	strain CFH S0261T	1078:1094	strain CFH S0261T	1078:1094	The cell wall diamino acid in the peptidoglycan of strain CFH S0261T was meso-diaminopimelic acid and the whole-cell hydrolysate comprised arabinose, galactose, glucose, rhamnose and ribose.
32250240	7	29	theme	gene	553:556	arg1	similarity					567:576	The 16S rRNA gene sequence similarity	540:576	The 16S rRNA gene sequence similarity	540:576	The 16S rRNA gene sequence similarity indicated that strain CFH S0261T is most closely related to the type strains of Amycolatopsis niigatensis LC11T (98.95 %), Amycolatopsis echigonensis LC2T (98.81 %) and Amycolatopsis albidoflavus IMSNU 22139T (98.73 %).
32250240	7	30	theme	16S	544:546	arg1	similarity					567:576	The 16S rRNA gene sequence similarity	540:576	The 16S rRNA gene sequence similarity	540:576	The 16S rRNA gene sequence similarity indicated that strain CFH S0261T is most closely related to the type strains of Amycolatopsis niigatensis LC11T (98.95 %), Amycolatopsis echigonensis LC2T (98.81 %) and Amycolatopsis albidoflavus IMSNU 22139T (98.73 %).
32250240	1	31	theme	Yellow	46:51	arg1	sample					59:64	a Yellow River sample	44:64	a Yellow River sample	44:64	nov., isolated from a Yellow River sample.
32250240	9	32	dep	low	989:991	arg1	%					1023:1023	ANIb <90.61 % and DDH <53.40 %	994:1023	ANIb <90.61 % and DDH <53.40 %	994:1023	The ANI values and in silico DDH values between CFH S0261T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %).
32250240	9	32	dep	low	989:991	arg1	values					872:877	The ANI values	864:877	The ANI values	864:877	The ANI values and in silico DDH values between CFH S0261T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %).
32250240	9	32	dep	low	989:991	arg1	low					989:991	low	989:991	low	989:991	The ANI values and in silico DDH values between CFH S0261T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %).
32250240	14	33	theme	name	1677:1680	arg1	sp					1702:1703	the name Amycolatopsis nivea sp	1673:1703	the name Amycolatopsis nivea sp	1673:1703	On the basis of phenotypic, genotypic and phylogenetic data, strain CFH S0261T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis nivea sp.
32250240	5	34	dep	%	395:395	arg1	w/v					398:400	w/v	398:400	w/v	398:400	Growth occurs at 15-37 °C, pH 6.0-8.0 and with 0-9.0 % (w/v) NaCl.
32250240	10	35	theme	meso-diaminopimelic	1100:1118	arg1	acid					1120:1123	meso-diaminopimelic acid	1100:1123	meso-diaminopimelic acid	1100:1123	The cell wall diamino acid in the peptidoglycan of strain CFH S0261T was meso-diaminopimelic acid and the whole-cell hydrolysate comprised arabinose, galactose, glucose, rhamnose and ribose.
32250240	10	35	theme	meso-diaminopimelic	1100:1118	arg1	acid					1049:1052	The cell wall diamino acid	1027:1052	The cell wall diamino acid in the peptidoglycan of strain CFH S0261T	1027:1094	The cell wall diamino acid in the peptidoglycan of strain CFH S0261T was meso-diaminopimelic acid and the whole-cell hydrolysate comprised arabinose, galactose, glucose, rhamnose and ribose.
32250240	9	36	dep	in	883:884	arg1	silico					886:891	silico	886:891	silico	886:891	The ANI values and in silico DDH values between CFH S0261T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %).
32250240	11	37	theme	predominant	1222:1232	arg1	menaquinone					1234:1244	The predominant menaquinone	1218:1244	The predominant menaquinone	1218:1244	The predominant menaquinone was MK-9(H4).
32250240	11	37	theme	predominant	1222:1232	arg1	MK-9					1250:1253	MK-9	1250:1253	MK-9(H4)	1250:1257	The predominant menaquinone was MK-9(H4).
32250240	10	38	theme	S0261T	1089:1094	arg1	peptidoglycan					1061:1073	the peptidoglycan	1057:1073	the peptidoglycan of strain CFH S0261T	1057:1094	The cell wall diamino acid in the peptidoglycan of strain CFH S0261T was meso-diaminopimelic acid and the whole-cell hydrolysate comprised arabinose, galactose, glucose, rhamnose and ribose.
32250240	0	39	theme	Amycolatopsis	0:12	arg1	sp					20:21	Amycolatopsis nivea sp	0:21	Amycolatopsis nivea sp.	0:22	Amycolatopsis nivea sp.
32250240	7	40	theme	echigonensis	715:726	arg1	%					740:740	98.81 %	734:740	98.81 %	734:740	The 16S rRNA gene sequence similarity indicated that strain CFH S0261T is most closely related to the type strains of Amycolatopsis niigatensis LC11T (98.95 %), Amycolatopsis echigonensis LC2T (98.81 %) and Amycolatopsis albidoflavus IMSNU 22139T (98.73 %).
32250240	7	40	theme	echigonensis	715:726	arg1	LC2T					728:731	Amycolatopsis echigonensis LC2T	701:731	Amycolatopsis echigonensis LC2T (98.81 %)	701:741	The 16S rRNA gene sequence similarity indicated that strain CFH S0261T is most closely related to the type strains of Amycolatopsis niigatensis LC11T (98.95 %), Amycolatopsis echigonensis LC2T (98.81 %) and Amycolatopsis albidoflavus IMSNU 22139T (98.73 %).
32250240	10	41	theme	whole-cell	1133:1142	arg1	hydrolysate					1144:1154	the whole-cell hydrolysate	1129:1154	the whole-cell hydrolysate	1129:1154	The cell wall diamino acid in the peptidoglycan of strain CFH S0261T was meso-diaminopimelic acid and the whole-cell hydrolysate comprised arabinose, galactose, glucose, rhamnose and ribose.
32250240	2	42	theme	sediment	142:149	arg1	sample					151:156	a sediment sample	140:156	a sediment sample of the Yellow River	140:176	A novel actinobacterium, designated strain CFH S0261T, was isolated from a sediment sample of the Yellow River.
32250240	13	43	contain	contained	1358:1366	arg2	phosphatidylethanolamine					1414:1437	phosphatidylethanolamine	1414:1437	phosphatidylethanolamine	1414:1437	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and four unidentified glycolipids.
32250240	13	43	contain	contained	1358:1366	arg2	glycolipids					1516:1526	four unidentified glycolipids	1498:1526	four unidentified glycolipids	1498:1526	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and four unidentified glycolipids.
32250240	13	43	contain	contained	1358:1366	arg2	mannosides					1483:1492	phosphatidylinositol mannosides	1462:1492	phosphatidylinositol mannosides	1462:1492	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and four unidentified glycolipids.
32250240	13	43	contain	contained	1358:1366	arg2	diphosphatidylglycerol					1368:1389	diphosphatidylglycerol	1368:1389	diphosphatidylglycerol	1368:1389	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and four unidentified glycolipids.
32250240	13	43	contain	contained	1358:1366	arg2	phosphatidylinositol					1440:1459	phosphatidylinositol	1440:1459	phosphatidylinositol	1440:1459	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and four unidentified glycolipids.
32250240	13	43	contain	contained	1358:1366	arg2	phosphatidylglycerol					1392:1411	phosphatidylglycerol	1392:1411	phosphatidylglycerol	1392:1411	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and four unidentified glycolipids.
32250240	13	43	contain	contained	1358:1366	arg1	profile					1350:1356	The polar lipid profile	1334:1356	The polar lipid profile	1334:1356	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and four unidentified glycolipids.
32250240	8	44	theme	CFH	818:820	arg1	S0261T					822:827	CFH S0261T	818:827	CFH S0261T	818:827	The whole-genome of CFH S0261T showed a G+C content of 69.5 mol%.
32250240	6	45	theme	Phylogenetic	409:420	arg1	analysis					422:429	Phylogenetic analysis	409:429	Phylogenetic analysis based on the 16S rRNA gene sequence	409:465	Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CFH S0261T was a member of the genus Amycolatopsis.
32250240	2	46	attach	isolated	126:133	arg2	actinobacterium					75:89	A novel actinobacterium	67:89	A novel actinobacterium	67:89	A novel actinobacterium, designated strain CFH S0261T, was isolated from a sediment sample of the Yellow River.
32250240	2	46	attach	isolated	126:133	arg1	sample					151:156	a sediment sample	140:156	a sediment sample of the Yellow River	140:176	A novel actinobacterium, designated strain CFH S0261T, was isolated from a sediment sample of the Yellow River.
32250240	2	47	theme	Yellow	165:170	arg1	River					172:176	the Yellow River	161:176	the Yellow River	161:176	A novel actinobacterium, designated strain CFH S0261T, was isolated from a sediment sample of the Yellow River.
32250240	5	48	theme	%	395:395	arg1	NaCl					403:406	0-9.0 % (w/v) NaCl	389:406	0-9.0 % (w/v) NaCl	389:406	Growth occurs at 15-37 °C, pH 6.0-8.0 and with 0-9.0 % (w/v) NaCl.
32250240	7	49	theme	niigatensis	672:682	arg1	%					697:697	98.95 %	691:697	98.95 %	691:697	The 16S rRNA gene sequence similarity indicated that strain CFH S0261T is most closely related to the type strains of Amycolatopsis niigatensis LC11T (98.95 %), Amycolatopsis echigonensis LC2T (98.81 %) and Amycolatopsis albidoflavus IMSNU 22139T (98.73 %).
32250240	7	49	theme	niigatensis	672:682	arg1	LC11T					684:688	Amycolatopsis niigatensis LC11T	658:688	Amycolatopsis niigatensis LC11T (98.95 %)	658:698	The 16S rRNA gene sequence similarity indicated that strain CFH S0261T is most closely related to the type strains of Amycolatopsis niigatensis LC11T (98.95 %), Amycolatopsis echigonensis LC2T (98.81 %) and Amycolatopsis albidoflavus IMSNU 22139T (98.73 %).
32250240	16	50	theme	=KCTC	1755:1759	arg1	S0261T					1747:1752	CFH S0261T	1743:1752	CFH S0261T (=KCTC 39515T =CCTCC AA 2014028T)	1743:1786	The type strain is CFH S0261T (=KCTC 39515T =CCTCC AA 2014028T).
32250240	16	50	theme	=KCTC	1755:1759	arg1	2014028T					1778:1785	=KCTC 39515T =CCTCC AA 2014028T	1755:1785	=KCTC 39515T =CCTCC AA 2014028T	1755:1785	The type strain is CFH S0261T (=KCTC 39515T =CCTCC AA 2014028T).
32250240	8	51	theme	G+C	838:840	arg1	content					842:848	a G+C content	836:848	a G+C content of 69.5 mol%	836:861	The whole-genome of CFH S0261T showed a G+C content of 69.5 mol%.
32250240	6	52	theme	CFH	488:490	arg1	S0261T					492:497	strain CFH S0261T	481:497	strain CFH S0261T	481:497	Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CFH S0261T was a member of the genus Amycolatopsis.
32250240	6	52	theme	CFH	488:490	arg1	member					505:510	a member	503:510	a member of the genus Amycolatopsis	503:537	Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CFH S0261T was a member of the genus Amycolatopsis.
32250240	9	53	theme	<90.61 	999:1005	arg1	%					1023:1023	ANIb <90.61 % and DDH <53.40 %	994:1023	ANIb <90.61 % and DDH <53.40 %	994:1023	The ANI values and in silico DDH values between CFH S0261T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %).
32250240	9	53	theme	<90.61 	999:1005	arg1	values					872:877	The ANI values	864:877	The ANI values	864:877	The ANI values and in silico DDH values between CFH S0261T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %).
32250240	9	53	theme	<90.61 	999:1005	arg1	low					989:991	low	989:991	low	989:991	The ANI values and in silico DDH values between CFH S0261T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %).
32250240	14	54	theme	strain	1590:1595	arg1	S0261T					1601:1606	strain CFH S0261T	1590:1606	strain CFH S0261T	1590:1606	On the basis of phenotypic, genotypic and phylogenetic data, strain CFH S0261T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis nivea sp.
32250240	12	55	theme	major	1264:1268	arg1	C16 					1296:1299	C16 	1296:1299	C16 	1296:1299	The major cellular fatty acids were C16 : 0, iso-C15 : 0 and iso-C16 : 0.
32250240	12	55	theme	major	1264:1268	arg1	acids					1285:1289	The major cellular fatty acids	1260:1289	The major cellular fatty acids	1260:1289	The major cellular fatty acids were C16 : 0, iso-C15 : 0 and iso-C16 : 0.
32250240	16	56	theme	=CCTCC	1768:1773	arg1	S0261T					1747:1752	CFH S0261T	1743:1752	CFH S0261T (=KCTC 39515T =CCTCC AA 2014028T)	1743:1786	The type strain is CFH S0261T (=KCTC 39515T =CCTCC AA 2014028T).
32250240	16	56	theme	=CCTCC	1768:1773	arg1	2014028T					1778:1785	=KCTC 39515T =CCTCC AA 2014028T	1755:1785	=KCTC 39515T =CCTCC AA 2014028T	1755:1785	The type strain is CFH S0261T (=KCTC 39515T =CCTCC AA 2014028T).
32250240	4	57	theme	S0261T	285:290	arg1	Cells					265:269	Cells	265:269	Cells of strain CFH S0261T	265:290	Cells of strain CFH S0261T were Gram-reaction-positive, aerobic, non-motile.
32250240	2	58	theme	strain	103:108	arg1	S0261T					114:119	strain CFH S0261T	103:119	strain CFH S0261T	103:119	A novel actinobacterium, designated strain CFH S0261T, was isolated from a sediment sample of the Yellow River.
32250240	9	59	theme	 and	1007:1010	arg1	%					1023:1023	ANIb <90.61 % and DDH <53.40 %	994:1023	ANIb <90.61 % and DDH <53.40 %	994:1023	The ANI values and in silico DDH values between CFH S0261T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %).
32250240	9	59	theme	 and	1007:1010	arg1	values					872:877	The ANI values	864:877	The ANI values	864:877	The ANI values and in silico DDH values between CFH S0261T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %).
32250240	9	59	theme	 and	1007:1010	arg1	low					989:991	low	989:991	low	989:991	The ANI values and in silico DDH values between CFH S0261T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %).
32250240	7	60	theme	type	642:645	arg1	strains					647:653	the type strains	638:653	the type strains of Amycolatopsis niigatensis LC11T (98.95 %), Amycolatopsis echigonensis LC2T (98.81 %) and Amycolatopsis albidoflavus IMSNU 22139T (98.73 %)	638:795	The 16S rRNA gene sequence similarity indicated that strain CFH S0261T is most closely related to the type strains of Amycolatopsis niigatensis LC11T (98.95 %), Amycolatopsis echigonensis LC2T (98.81 %) and Amycolatopsis albidoflavus IMSNU 22139T (98.73 %).
32250240	12	61	theme	fatty	1279:1283	arg1	C16 					1296:1299	C16 	1296:1299	C16 	1296:1299	The major cellular fatty acids were C16 : 0, iso-C15 : 0 and iso-C16 : 0.
32250240	12	61	theme	fatty	1279:1283	arg1	acids					1285:1289	The major cellular fatty acids	1260:1289	The major cellular fatty acids	1260:1289	The major cellular fatty acids were C16 : 0, iso-C15 : 0 and iso-C16 : 0.
32250240	8	62	theme	%	861:861	arg1	content					842:848	a G+C content	836:848	a G+C content of 69.5 mol%	836:861	The whole-genome of CFH S0261T showed a G+C content of 69.5 mol%.
32250240	6	63	theme	rRNA	448:451	arg1	sequence					458:465	the 16S rRNA gene sequence	440:465	the 16S rRNA gene sequence	440:465	Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CFH S0261T was a member of the genus Amycolatopsis.
32250240	7	64	theme	IMSNU	774:778	arg1	22139T					780:785	Amycolatopsis albidoflavus IMSNU 22139T	747:785	Amycolatopsis albidoflavus IMSNU 22139T (98.73 %)	747:795	The 16S rRNA gene sequence similarity indicated that strain CFH S0261T is most closely related to the type strains of Amycolatopsis niigatensis LC11T (98.95 %), Amycolatopsis echigonensis LC2T (98.81 %) and Amycolatopsis albidoflavus IMSNU 22139T (98.73 %).
32250240	7	64	theme	IMSNU	774:778	arg1	%					794:794	98.73 %	788:794	98.73 %	788:794	The 16S rRNA gene sequence similarity indicated that strain CFH S0261T is most closely related to the type strains of Amycolatopsis niigatensis LC11T (98.95 %), Amycolatopsis echigonensis LC2T (98.81 %) and Amycolatopsis albidoflavus IMSNU 22139T (98.73 %).
32250240	6	65	theme	Amycolatopsis	525:537	arg1	S0261T					492:497	strain CFH S0261T	481:497	strain CFH S0261T	481:497	Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CFH S0261T was a member of the genus Amycolatopsis.
32250240	6	65	theme	Amycolatopsis	525:537	arg1	member					505:510	a member	503:510	a member of the genus Amycolatopsis	503:537	Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CFH S0261T was a member of the genus Amycolatopsis.
32250240	13	66	theme	lipid	1344:1348	arg1	profile					1350:1356	The polar lipid profile	1334:1356	The polar lipid profile	1334:1356	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and four unidentified glycolipids.
32250240	7	67	theme	Amycolatopsis	747:759	arg1	22139T					780:785	Amycolatopsis albidoflavus IMSNU 22139T	747:785	Amycolatopsis albidoflavus IMSNU 22139T (98.73 %)	747:795	The 16S rRNA gene sequence similarity indicated that strain CFH S0261T is most closely related to the type strains of Amycolatopsis niigatensis LC11T (98.95 %), Amycolatopsis echigonensis LC2T (98.81 %) and Amycolatopsis albidoflavus IMSNU 22139T (98.73 %).
32250240	7	67	theme	Amycolatopsis	747:759	arg1	%					794:794	98.73 %	788:794	98.73 %	788:794	The 16S rRNA gene sequence similarity indicated that strain CFH S0261T is most closely related to the type strains of Amycolatopsis niigatensis LC11T (98.95 %), Amycolatopsis echigonensis LC2T (98.81 %) and Amycolatopsis albidoflavus IMSNU 22139T (98.73 %).
32250240	9	68	theme	CFH	912:914	arg1	S0261T					916:921	CFH S0261T	912:921	CFH S0261T	912:921	The ANI values and in silico DDH values between CFH S0261T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %).
32250240	9	69	theme	ANI	868:870	arg1	%					1023:1023	ANIb <90.61 % and DDH <53.40 %	994:1023	ANIb <90.61 % and DDH <53.40 %	994:1023	The ANI values and in silico DDH values between CFH S0261T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %).
32250240	9	69	theme	ANI	868:870	arg1	values					872:877	The ANI values	864:877	The ANI values	864:877	The ANI values and in silico DDH values between CFH S0261T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %).
32250240	9	69	theme	ANI	868:870	arg1	low					989:991	low	989:991	low	989:991	The ANI values and in silico DDH values between CFH S0261T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %).
32250240	14	70	theme	phenotypic	1545:1554	arg1	data					1584:1587	phenotypic, genotypic and phylogenetic data	1545:1587	phenotypic, genotypic and phylogenetic data	1545:1587	On the basis of phenotypic, genotypic and phylogenetic data, strain CFH S0261T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis nivea sp.
32250240	2	71	theme	novel	69:73	arg1	actinobacterium					75:89	A novel actinobacterium	67:89	A novel actinobacterium	67:89	A novel actinobacterium, designated strain CFH S0261T, was isolated from a sediment sample of the Yellow River.
32250240	14	72	theme	Amycolatopsis	1682:1694	arg1	sp					1702:1703	the name Amycolatopsis nivea sp	1673:1703	the name Amycolatopsis nivea sp	1673:1703	On the basis of phenotypic, genotypic and phylogenetic data, strain CFH S0261T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis nivea sp.
32250240	12	73	dep	C16 	1296:1299	arg1	iso-C16 					1321:1328	iso-C16 	1321:1328	iso-C16 	1321:1328	The major cellular fatty acids were C16 : 0, iso-C15 : 0 and iso-C16 : 0.
32250240	12	73	dep	C16 	1296:1299	arg1	 0					1314:1315	 0	1314:1315	 0	1314:1315	The major cellular fatty acids were C16 : 0, iso-C15 : 0 and iso-C16 : 0.
32250240	12	73	dep	C16 	1296:1299	arg1	 0					1301:1302	 0	1301:1302	 0	1301:1302	The major cellular fatty acids were C16 : 0, iso-C15 : 0 and iso-C16 : 0.
32250240	12	73	dep	C16 	1296:1299	arg1	 0					1330:1331	 0	1330:1331	 0	1330:1331	The major cellular fatty acids were C16 : 0, iso-C15 : 0 and iso-C16 : 0.
32250240	7	74	theme	strain	593:598	arg1	S0261T					604:609	strain CFH S0261T	593:609	strain CFH S0261T	593:609	The 16S rRNA gene sequence similarity indicated that strain CFH S0261T is most closely related to the type strains of Amycolatopsis niigatensis LC11T (98.95 %), Amycolatopsis echigonensis LC2T (98.81 %) and Amycolatopsis albidoflavus IMSNU 22139T (98.73 %).
32250240	10	75	theme	CFH	1085:1087	arg1	S0261T					1089:1094	strain CFH S0261T	1078:1094	strain CFH S0261T	1078:1094	The cell wall diamino acid in the peptidoglycan of strain CFH S0261T was meso-diaminopimelic acid and the whole-cell hydrolysate comprised arabinose, galactose, glucose, rhamnose and ribose.
32250240	7	76	theme	sequence	558:565	arg1	similarity					567:576	The 16S rRNA gene sequence similarity	540:576	The 16S rRNA gene sequence similarity	540:576	The 16S rRNA gene sequence similarity indicated that strain CFH S0261T is most closely related to the type strains of Amycolatopsis niigatensis LC11T (98.95 %), Amycolatopsis echigonensis LC2T (98.81 %) and Amycolatopsis albidoflavus IMSNU 22139T (98.73 %).
32250240	4	77	theme	strain	274:279	arg1	S0261T					285:290	strain CFH S0261T	274:290	strain CFH S0261T	274:290	Cells of strain CFH S0261T were Gram-reaction-positive, aerobic, non-motile.
32250240	9	78	theme	other	931:935	arg1	species					937:943	the other species	927:943	the other species of the genus Amycolatopsis	927:970	The ANI values and in silico DDH values between CFH S0261T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %).
32250240	7	79	theme	rRNA	548:551	arg1	similarity					567:576	The 16S rRNA gene sequence similarity	540:576	The 16S rRNA gene sequence similarity	540:576	The 16S rRNA gene sequence similarity indicated that strain CFH S0261T is most closely related to the type strains of Amycolatopsis niigatensis LC11T (98.95 %), Amycolatopsis echigonensis LC2T (98.81 %) and Amycolatopsis albidoflavus IMSNU 22139T (98.73 %).
32250240	1	80	theme	River	53:57	arg1	sample					59:64	a Yellow River sample	44:64	a Yellow River sample	44:64	nov., isolated from a Yellow River sample.
32250240	7	81	theme	LC11T	684:688	arg1	strains					647:653	the type strains	638:653	the type strains of Amycolatopsis niigatensis LC11T (98.95 %), Amycolatopsis echigonensis LC2T (98.81 %) and Amycolatopsis albidoflavus IMSNU 22139T (98.73 %)	638:795	The 16S rRNA gene sequence similarity indicated that strain CFH S0261T is most closely related to the type strains of Amycolatopsis niigatensis LC11T (98.95 %), Amycolatopsis echigonensis LC2T (98.81 %) and Amycolatopsis albidoflavus IMSNU 22139T (98.73 %).
32250240	0	82	theme	nivea	14:18	arg1	sp					20:21	Amycolatopsis nivea sp	0:21	Amycolatopsis nivea sp.	0:22	Amycolatopsis nivea sp.
32250240	9	83	theme	genus	952:956	arg1	Amycolatopsis					958:970	the genus Amycolatopsis	948:970	the genus Amycolatopsis	948:970	The ANI values and in silico DDH values between CFH S0261T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %).
32250240	7	84	theme	LC2T	728:731	arg1	strains					647:653	the type strains	638:653	the type strains of Amycolatopsis niigatensis LC11T (98.95 %), Amycolatopsis echigonensis LC2T (98.81 %) and Amycolatopsis albidoflavus IMSNU 22139T (98.73 %)	638:795	The 16S rRNA gene sequence similarity indicated that strain CFH S0261T is most closely related to the type strains of Amycolatopsis niigatensis LC11T (98.95 %), Amycolatopsis echigonensis LC2T (98.81 %) and Amycolatopsis albidoflavus IMSNU 22139T (98.73 %).
32250240	4	85	dep	Gram-reaction-positive	297:318	arg1	non-motile					330:339	non-motile	330:339	non-motile	330:339	Cells of strain CFH S0261T were Gram-reaction-positive, aerobic, non-motile.
32250240	4	85	dep	Gram-reaction-positive	297:318	arg1	aerobic					321:327	aerobic	321:327	aerobic	321:327	Cells of strain CFH S0261T were Gram-reaction-positive, aerobic, non-motile.
32250240	7	86	theme	Amycolatopsis	701:713	arg1	%					740:740	98.81 %	734:740	98.81 %	734:740	The 16S rRNA gene sequence similarity indicated that strain CFH S0261T is most closely related to the type strains of Amycolatopsis niigatensis LC11T (98.95 %), Amycolatopsis echigonensis LC2T (98.81 %) and Amycolatopsis albidoflavus IMSNU 22139T (98.73 %).
32250240	7	86	theme	Amycolatopsis	701:713	arg1	LC2T					728:731	Amycolatopsis echigonensis LC2T	701:731	Amycolatopsis echigonensis LC2T (98.81 %)	701:741	The 16S rRNA gene sequence similarity indicated that strain CFH S0261T is most closely related to the type strains of Amycolatopsis niigatensis LC11T (98.95 %), Amycolatopsis echigonensis LC2T (98.81 %) and Amycolatopsis albidoflavus IMSNU 22139T (98.73 %).
32250240	16	87	theme	CFH	1743:1745	arg1	S0261T					1747:1752	CFH S0261T	1743:1752	CFH S0261T (=KCTC 39515T =CCTCC AA 2014028T)	1743:1786	The type strain is CFH S0261T (=KCTC 39515T =CCTCC AA 2014028T).
32250240	16	87	theme	CFH	1743:1745	arg1	2014028T					1778:1785	=KCTC 39515T =CCTCC AA 2014028T	1755:1785	=KCTC 39515T =CCTCC AA 2014028T	1755:1785	The type strain is CFH S0261T (=KCTC 39515T =CCTCC AA 2014028T).
32250240	16	87	theme	CFH	1743:1745	arg1	strain					1733:1738	The type strain	1724:1738	The type strain	1724:1738	The type strain is CFH S0261T (=KCTC 39515T =CCTCC AA 2014028T).
32250240	13	88	theme	unidentified	1503:1514	arg1	glycolipids					1516:1526	four unidentified glycolipids	1498:1526	four unidentified glycolipids	1498:1526	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and four unidentified glycolipids.
32250240	14	89	theme	novel	1621:1625	arg1	species					1627:1633	a novel species	1619:1633	a novel species	1619:1633	On the basis of phenotypic, genotypic and phylogenetic data, strain CFH S0261T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis nivea sp.
32250240	2	90	theme	River	172:176	arg1	sample					151:156	a sediment sample	140:156	a sediment sample of the Yellow River	140:176	A novel actinobacterium, designated strain CFH S0261T, was isolated from a sediment sample of the Yellow River.
32250240	5	91	theme	0-9.0 	389:394	arg1	%					395:395	%	395:395	%	395:395	Growth occurs at 15-37 °C, pH 6.0-8.0 and with 0-9.0 % (w/v) NaCl.
32250240	16	92	theme	39515T	1761:1766	arg1	S0261T					1747:1752	CFH S0261T	1743:1752	CFH S0261T (=KCTC 39515T =CCTCC AA 2014028T)	1743:1786	The type strain is CFH S0261T (=KCTC 39515T =CCTCC AA 2014028T).
32250240	16	92	theme	39515T	1761:1766	arg1	2014028T					1778:1785	=KCTC 39515T =CCTCC AA 2014028T	1755:1785	=KCTC 39515T =CCTCC AA 2014028T	1755:1785	The type strain is CFH S0261T (=KCTC 39515T =CCTCC AA 2014028T).
32250240	8	93	theme	S0261T	822:827	arg1	whole-genome					802:813	The whole-genome	798:813	The whole-genome of CFH S0261T	798:827	The whole-genome of CFH S0261T showed a G+C content of 69.5 mol%.
32250240	13	94	theme	phosphatidylinositol	1462:1481	arg1	mannosides					1483:1492	phosphatidylinositol mannosides	1462:1492	phosphatidylinositol mannosides	1462:1492	The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides and four unidentified glycolipids.
32250240	9	95	theme	ANIb	994:997	arg1	%					1023:1023	ANIb <90.61 % and DDH <53.40 %	994:1023	ANIb <90.61 % and DDH <53.40 %	994:1023	The ANI values and in silico DDH values between CFH S0261T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %).
32250240	9	95	theme	ANIb	994:997	arg1	values					872:877	The ANI values	864:877	The ANI values	864:877	The ANI values and in silico DDH values between CFH S0261T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %).
32250240	9	95	theme	ANIb	994:997	arg1	low					989:991	low	989:991	low	989:991	The ANI values and in silico DDH values between CFH S0261T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %).
32250240	14	96	theme	CFH	1597:1599	arg1	S0261T					1601:1606	strain CFH S0261T	1590:1606	strain CFH S0261T	1590:1606	On the basis of phenotypic, genotypic and phylogenetic data, strain CFH S0261T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis nivea sp.
32250240	16	97	theme	AA	1775:1776	arg1	S0261T					1747:1752	CFH S0261T	1743:1752	CFH S0261T (=KCTC 39515T =CCTCC AA 2014028T)	1743:1786	The type strain is CFH S0261T (=KCTC 39515T =CCTCC AA 2014028T).
32250240	16	97	theme	AA	1775:1776	arg1	2014028T					1778:1785	=KCTC 39515T =CCTCC AA 2014028T	1755:1785	=KCTC 39515T =CCTCC AA 2014028T	1755:1785	The type strain is CFH S0261T (=KCTC 39515T =CCTCC AA 2014028T).
32250240	6	98	theme	strain	481:486	arg1	S0261T					492:497	strain CFH S0261T	481:497	strain CFH S0261T	481:497	Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CFH S0261T was a member of the genus Amycolatopsis.
32250240	6	98	theme	strain	481:486	arg1	member					505:510	a member	503:510	a member of the genus Amycolatopsis	503:537	Phylogenetic analysis based on the 16S rRNA gene sequence revealed that strain CFH S0261T was a member of the genus Amycolatopsis.
32250240	2	99	theme	CFH	110:112	arg1	S0261T					114:119	strain CFH S0261T	103:119	strain CFH S0261T	103:119	A novel actinobacterium, designated strain CFH S0261T, was isolated from a sediment sample of the Yellow River.
32250240	9	100	theme	%	1006:1006	arg1	%					1023:1023	ANIb <90.61 % and DDH <53.40 %	994:1023	ANIb <90.61 % and DDH <53.40 %	994:1023	The ANI values and in silico DDH values between CFH S0261T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %).
32250240	9	100	theme	%	1006:1006	arg1	values					872:877	The ANI values	864:877	The ANI values	864:877	The ANI values and in silico DDH values between CFH S0261T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %).
32250240	9	100	theme	%	1006:1006	arg1	low					989:991	low	989:991	low	989:991	The ANI values and in silico DDH values between CFH S0261T and the other species of the genus Amycolatopsis were found to be low (ANIb <90.61 % and DDH <53.40 %).
32250240	7	101	theme	Amycolatopsis	658:670	arg1	%					697:697	98.95 %	691:697	98.95 %	691:697	The 16S rRNA gene sequence similarity indicated that strain CFH S0261T is most closely related to the type strains of Amycolatopsis niigatensis LC11T (98.95 %), Amycolatopsis echigonensis LC2T (98.81 %) and Amycolatopsis albidoflavus IMSNU 22139T (98.73 %).
32250240	7	101	theme	Amycolatopsis	658:670	arg1	LC11T					684:688	Amycolatopsis niigatensis LC11T	658:688	Amycolatopsis niigatensis LC11T (98.95 %)	658:698	The 16S rRNA gene sequence similarity indicated that strain CFH S0261T is most closely related to the type strains of Amycolatopsis niigatensis LC11T (98.95 %), Amycolatopsis echigonensis LC2T (98.81 %) and Amycolatopsis albidoflavus IMSNU 22139T (98.73 %).
32238231	2	0	theme	non-motile	123:132	arg1	strain					134:139	A Gram-stain-positive, facultatively anaerobic and non-motile strain	72:139	A Gram-stain-positive, facultatively anaerobic and non-motile strain	72:139	A Gram-stain-positive, facultatively anaerobic and non-motile strain, designated SYSUP0004T, was isolated from the tubers of Gastrodia elata Blume collected from Yunnan Province, PR China.
32238231	4	1	theme	NaCl	510:513	arg1	 w/v					524:527	NaCl up to 4 % w/v	510:527	NaCl up to 4 % w/v (optimum in the absence of NaCl)	510:560	SYSUP0004T grew at pH 6.0-9.0 (optimum, pH 8.0), temperature 4-30 °C (optimum, 28 °C) and could tolerate NaCl up to 4 % w/v (optimum in the absence of NaCl).
32238231	4	2	dep	optimum	475:481	arg1	28 °C					484:488	28 °C	484:488	28 °C	484:488	SYSUP0004T grew at pH 6.0-9.0 (optimum, pH 8.0), temperature 4-30 °C (optimum, 28 °C) and could tolerate NaCl up to 4 % w/v (optimum in the absence of NaCl).
32238231	4	3	theme	temperature	454:464	arg1	optimum					475:481	optimum	475:481	optimum	475:481	SYSUP0004T grew at pH 6.0-9.0 (optimum, pH 8.0), temperature 4-30 °C (optimum, 28 °C) and could tolerate NaCl up to 4 % w/v (optimum in the absence of NaCl).
32238231	4	3	theme	temperature	454:464	arg1	4-30 °C					466:472	temperature 4-30 °C	454:472	temperature 4-30 °C (optimum, 28 °C)	454:489	SYSUP0004T grew at pH 6.0-9.0 (optimum, pH 8.0), temperature 4-30 °C (optimum, 28 °C) and could tolerate NaCl up to 4 % w/v (optimum in the absence of NaCl).
32238231	4	3	theme	temperature	454:464	arg1	pH					424:425	pH 6.0-9.0	424:433	pH 6.0-9.0 (optimum, pH 8.0)	424:451	SYSUP0004T grew at pH 6.0-9.0 (optimum, pH 8.0), temperature 4-30 °C (optimum, 28 °C) and could tolerate NaCl up to 4 % w/v (optimum in the absence of NaCl).
32238231	8	4	dep	anteiso-C15:0	788:800	arg1	C16 					822:825	C16 	822:825	C16 	822:825	The major fatty acids were anteiso-C15:0, anteiso-C15 : 1 A, C16 : 0 and anteiso-C17 : 0.
32238231	8	4	dep	anteiso-C15:0	788:800	arg1	A					819:819	A	819:819	A	819:819	The major fatty acids were anteiso-C15:0, anteiso-C15 : 1 A, C16 : 0 and anteiso-C17 : 0.
32238231	9	5	theme	unidentified	911:922	arg1	phosphoglycolipid					924:940	unidentified phosphoglycolipid	911:940	unidentified phosphoglycolipid	911:940	The polar lipids of SYSUP0004T were diphosphatidylglycerol, unidentified phosphoglycolipid, phosphatidylinositol mannosides and unidentified glycolipid.
32238231	5	6	theme	interpeptide	612:623	arg1	acid					655:658	an interpeptide bridge l-ornithine-d-glutamic acid	609:658	an interpeptide bridge l-ornithine-d-glutamic acid	609:658	The cell-wall peptidoglycan type was A4β with an interpeptide bridge l-ornithine-d-glutamic acid.
32238231	2	7	theme	anaerobic	109:117	arg1	strain					134:139	A Gram-stain-positive, facultatively anaerobic and non-motile strain	72:139	A Gram-stain-positive, facultatively anaerobic and non-motile strain	72:139	A Gram-stain-positive, facultatively anaerobic and non-motile strain, designated SYSUP0004T, was isolated from the tubers of Gastrodia elata Blume collected from Yunnan Province, PR China.
32238231	11	8	theme	cut-off	1155:1161	arg1	level					1163:1167	the cut-off level	1151:1167	the cut-off level (95-96 %)	1151:1177	The average nucleotide identity values between SYSUP0004T and members of the genus Cellulomonas were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity.
32238231	11	8	theme	cut-off	1155:1161	arg1	%					1176:1176	95-96 %	1170:1176	95-96 %	1170:1176	The average nucleotide identity values between SYSUP0004T and members of the genus Cellulomonas were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity.
32238231	14	9	theme	type	1414:1417	arg1	strain					1419:1424	The type strain	1410:1424	The type strain	1410:1424	The type strain, SYSUP0004T (=KCTC 49025T=CGMCC 1.16405T).
32238231	1	10	theme	Gastrodia	49:57	arg1	elata					59:63	Gastrodia elata	49:63	Gastrodia elata Blume	49:69	nov., isolated from Gastrodia elata Blume.
32238231	10	11	theme	DNA	1016:1018	arg1	content					1024:1030	The genomic DNA G+C content	1004:1030	The genomic DNA G+C content	1004:1030	The genomic DNA G+C content was 76.5 %.
32238231	10	11	theme	DNA	1016:1018	arg1	%					1041:1041	76.5 %	1036:1041	76.5 %	1036:1041	The genomic DNA G+C content was 76.5 %.
32238231	5	12	theme	bridge	625:630	arg1	acid					655:658	an interpeptide bridge l-ornithine-d-glutamic acid	609:658	an interpeptide bridge l-ornithine-d-glutamic acid	609:658	The cell-wall peptidoglycan type was A4β with an interpeptide bridge l-ornithine-d-glutamic acid.
32238231	11	13	theme	identity	1067:1074	arg1	criterion					1202:1210	the ANI criterion	1194:1210	the ANI criterion for interspecies identity	1194:1236	The average nucleotide identity values between SYSUP0004T and members of the genus Cellulomonas were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity.
32238231	11	13	theme	identity	1067:1074	arg1	values					1076:1081	The average nucleotide identity values	1044:1081	The average nucleotide identity values between SYSUP0004T and members of the genus Cellulomonas	1044:1138	The average nucleotide identity values between SYSUP0004T and members of the genus Cellulomonas were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity.
32238231	11	14	theme	Cellulomonas	1127:1138	arg1	members					1106:1112	members	1106:1112	members of the genus Cellulomonas	1106:1138	The average nucleotide identity values between SYSUP0004T and members of the genus Cellulomonas were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity.
32238231	11	14	theme	Cellulomonas	1127:1138	arg1	SYSUP0004T					1091:1100	SYSUP0004T	1091:1100	SYSUP0004T	1091:1100	The average nucleotide identity values between SYSUP0004T and members of the genus Cellulomonas were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity.
32238231	1	15	theme	elata	59:63	arg1	Blume					65:69	Gastrodia elata Blume	49:69	Gastrodia elata Blume	49:69	nov., isolated from Gastrodia elata Blume.
32238231	5	16	theme	l-ornithine-d-glutamic	632:653	arg1	acid					655:658	an interpeptide bridge l-ornithine-d-glutamic acid	609:658	an interpeptide bridge l-ornithine-d-glutamic acid	609:658	The cell-wall peptidoglycan type was A4β with an interpeptide bridge l-ornithine-d-glutamic acid.
32238231	9	17	theme	phosphatidylinositol	943:962	arg1	mannosides					964:973	phosphatidylinositol mannosides	943:973	phosphatidylinositol mannosides	943:973	The polar lipids of SYSUP0004T were diphosphatidylglycerol, unidentified phosphoglycolipid, phosphatidylinositol mannosides and unidentified glycolipid.
32238231	4	18	theme	NaCl	556:559	arg1	absence					545:551	the absence	541:551	the absence of NaCl	541:559	SYSUP0004T grew at pH 6.0-9.0 (optimum, pH 8.0), temperature 4-30 °C (optimum, 28 °C) and could tolerate NaCl up to 4 % w/v (optimum in the absence of NaCl).
32238231	3	19	theme	low	336:338	arg1	similarity					340:349	low similarity	336:349	low similarity (97.7 %)	336:358	The 16S rRNA gene sequence result showed that the strain SYSUP0004T shared low similarity (97.7 %) with the type strain of Cellulomonas marina.
32238231	3	19	theme	low	336:338	arg1	%					357:357	97.7 %	352:357	97.7 %	352:357	The 16S rRNA gene sequence result showed that the strain SYSUP0004T shared low similarity (97.7 %) with the type strain of Cellulomonas marina.
32238231	11	20	theme	average	1048:1054	arg1	criterion					1202:1210	the ANI criterion	1194:1210	the ANI criterion for interspecies identity	1194:1236	The average nucleotide identity values between SYSUP0004T and members of the genus Cellulomonas were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity.
32238231	11	20	theme	average	1048:1054	arg1	values					1076:1081	The average nucleotide identity values	1044:1081	The average nucleotide identity values between SYSUP0004T and members of the genus Cellulomonas	1044:1138	The average nucleotide identity values between SYSUP0004T and members of the genus Cellulomonas were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity.
32238231	8	21	theme	major	765:769	arg1	acids					777:781	The major fatty acids	761:781	The major fatty acids	761:781	The major fatty acids were anteiso-C15:0, anteiso-C15 : 1 A, C16 : 0 and anteiso-C17 : 0.
32238231	8	21	theme	major	765:769	arg1	anteiso-C15:0					788:800	anteiso-C15:0	788:800	anteiso-C15:0	788:800	The major fatty acids were anteiso-C15:0, anteiso-C15 : 1 A, C16 : 0 and anteiso-C17 : 0.
32238231	0	22	theme	endophytica	13:23	arg1	sp					25:26	Cellulomonas endophytica sp	0:26	Cellulomonas endophytica sp.	0:27	Cellulomonas endophytica sp.
32238231	12	23	theme	novel	1303:1307	arg1	species					1309:1315	a novel species	1301:1315	a novel species	1301:1315	Thus, based on the above results strain SYSUP0004T represents a novel species of the genus Cellulomonas, for which the name Cellulomonas endophytica sp.
32238231	3	24	theme	Cellulomonas	384:395	arg1	marina					397:402	Cellulomonas marina	384:402	Cellulomonas marina	384:402	The 16S rRNA gene sequence result showed that the strain SYSUP0004T shared low similarity (97.7 %) with the type strain of Cellulomonas marina.
32238231	0	25	theme	Cellulomonas	0:11	arg1	sp					25:26	Cellulomonas endophytica sp	0:26	Cellulomonas endophytica sp.	0:27	Cellulomonas endophytica sp.
32238231	4	26	from	absence	545:551	arg1	optimum					530:536	optimum	530:536	optimum	530:536	SYSUP0004T grew at pH 6.0-9.0 (optimum, pH 8.0), temperature 4-30 °C (optimum, 28 °C) and could tolerate NaCl up to 4 % w/v (optimum in the absence of NaCl).
32238231	3	27	theme	marina	397:402	arg1	strain					374:379	the type strain	365:379	the type strain of Cellulomonas marina	365:402	The 16S rRNA gene sequence result showed that the strain SYSUP0004T shared low similarity (97.7 %) with the type strain of Cellulomonas marina.
32238231	10	28	theme	G+C	1020:1022	arg1	content					1024:1030	The genomic DNA G+C content	1004:1030	The genomic DNA G+C content	1004:1030	The genomic DNA G+C content was 76.5 %.
32238231	10	28	theme	G+C	1020:1022	arg1	%					1041:1041	76.5 %	1036:1041	76.5 %	1036:1041	The genomic DNA G+C content was 76.5 %.
32238231	3	29	theme	16S	265:267	arg1	rRNA					269:272	The 16S rRNA	261:272	The 16S rRNA gene sequence result	261:293	The 16S rRNA gene sequence result showed that the strain SYSUP0004T shared low similarity (97.7 %) with the type strain of Cellulomonas marina.
32238231	8	30	theme	fatty	771:775	arg1	acids					777:781	The major fatty acids	761:781	The major fatty acids	761:781	The major fatty acids were anteiso-C15:0, anteiso-C15 : 1 A, C16 : 0 and anteiso-C17 : 0.
32238231	8	30	theme	fatty	771:775	arg1	anteiso-C15:0					788:800	anteiso-C15:0	788:800	anteiso-C15:0	788:800	The major fatty acids were anteiso-C15:0, anteiso-C15 : 1 A, C16 : 0 and anteiso-C17 : 0.
32238231	11	31	theme	ANI	1198:1200	arg1	criterion					1202:1210	the ANI criterion	1194:1210	the ANI criterion for interspecies identity	1194:1236	The average nucleotide identity values between SYSUP0004T and members of the genus Cellulomonas were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity.
32238231	11	31	theme	ANI	1198:1200	arg1	values					1076:1081	The average nucleotide identity values	1044:1081	The average nucleotide identity values between SYSUP0004T and members of the genus Cellulomonas	1044:1138	The average nucleotide identity values between SYSUP0004T and members of the genus Cellulomonas were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity.
32238231	4	32	dep	optimum	436:442	arg1	pH					445:446	pH 8.0	445:450	pH 8.0	445:450	SYSUP0004T grew at pH 6.0-9.0 (optimum, pH 8.0), temperature 4-30 °C (optimum, 28 °C) and could tolerate NaCl up to 4 % w/v (optimum in the absence of NaCl).
32238231	3	33	theme	rRNA	269:272	arg1	result					288:293	The 16S rRNA gene sequence result	261:293	The 16S rRNA gene sequence result	261:293	The 16S rRNA gene sequence result showed that the strain SYSUP0004T shared low similarity (97.7 %) with the type strain of Cellulomonas marina.
32238231	12	34	theme	Cellulomonas	1363:1374	arg1	sp					1388:1389	the name Cellulomonas endophytica sp	1354:1389	the name Cellulomonas endophytica sp	1354:1389	Thus, based on the above results strain SYSUP0004T represents a novel species of the genus Cellulomonas, for which the name Cellulomonas endophytica sp.
32238231	4	35	theme	optimum	530:536	arg1	 w/v					524:527	NaCl up to 4 % w/v	510:527	NaCl up to 4 % w/v (optimum in the absence of NaCl)	510:560	SYSUP0004T grew at pH 6.0-9.0 (optimum, pH 8.0), temperature 4-30 °C (optimum, 28 °C) and could tolerate NaCl up to 4 % w/v (optimum in the absence of NaCl).
32238231	14	36	theme	=KCTC	1439:1443	arg1	1.16405T					1458:1465	=KCTC 49025T=CGMCC 1.16405T	1439:1465	=KCTC 49025T=CGMCC 1.16405T	1439:1465	The type strain, SYSUP0004T (=KCTC 49025T=CGMCC 1.16405T).
32238231	14	36	theme	=KCTC	1439:1443	arg1	SYSUP0004T					1427:1436	SYSUP0004T	1427:1436	SYSUP0004T (=KCTC 49025T=CGMCC 1.16405T)	1427:1466	The type strain, SYSUP0004T (=KCTC 49025T=CGMCC 1.16405T).
32238231	10	37	theme	genomic	1008:1014	arg1	content					1024:1030	The genomic DNA G+C content	1004:1030	The genomic DNA G+C content	1004:1030	The genomic DNA G+C content was 76.5 %.
32238231	10	37	theme	genomic	1008:1014	arg1	%					1041:1041	76.5 %	1036:1041	76.5 %	1036:1041	The genomic DNA G+C content was 76.5 %.
32238231	12	38	theme	name	1358:1361	arg1	sp					1388:1389	the name Cellulomonas endophytica sp	1354:1389	the name Cellulomonas endophytica sp	1354:1389	Thus, based on the above results strain SYSUP0004T represents a novel species of the genus Cellulomonas, for which the name Cellulomonas endophytica sp.
32238231	4	39	from	optimum	530:536	arg1	absence					545:551	the absence	541:551	the absence of NaCl	541:559	SYSUP0004T grew at pH 6.0-9.0 (optimum, pH 8.0), temperature 4-30 °C (optimum, 28 °C) and could tolerate NaCl up to 4 % w/v (optimum in the absence of NaCl).
32238231	12	40	theme	above	1258:1262	arg1	results					1264:1270	the above results	1254:1270	the above results	1254:1270	Thus, based on the above results strain SYSUP0004T represents a novel species of the genus Cellulomonas, for which the name Cellulomonas endophytica sp.
32238231	12	41	theme	Cellulomonas	1330:1341	arg1	species					1309:1315	a novel species	1301:1315	a novel species	1301:1315	Thus, based on the above results strain SYSUP0004T represents a novel species of the genus Cellulomonas, for which the name Cellulomonas endophytica sp.
32238231	14	42	dep	SYSUP0004T	1427:1436	arg1	strain					1419:1424	The type strain	1410:1424	The type strain	1410:1424	The type strain, SYSUP0004T (=KCTC 49025T=CGMCC 1.16405T).
32238231	2	43	attach	isolated	169:176	arg1	Blume					213:217	Blume	213:217	Blume	213:217	A Gram-stain-positive, facultatively anaerobic and non-motile strain, designated SYSUP0004T, was isolated from the tubers of Gastrodia elata Blume collected from Yunnan Province, PR China.
32238231	2	43	attach	isolated	169:176	arg2	strain					134:139	A Gram-stain-positive, facultatively anaerobic and non-motile strain	72:139	A Gram-stain-positive, facultatively anaerobic and non-motile strain	72:139	A Gram-stain-positive, facultatively anaerobic and non-motile strain, designated SYSUP0004T, was isolated from the tubers of Gastrodia elata Blume collected from Yunnan Province, PR China.
32238231	2	43	attach	isolated	169:176	arg1	tubers					187:192	the tubers	183:192	the tubers of Gastrodia elata Blume collected from Yunnan Province, PR China	183:258	A Gram-stain-positive, facultatively anaerobic and non-motile strain, designated SYSUP0004T, was isolated from the tubers of Gastrodia elata Blume collected from Yunnan Province, PR China.
32238231	11	44	theme	interspecies	1216:1227	arg1	identity					1229:1236	interspecies identity	1216:1236	interspecies identity	1216:1236	The average nucleotide identity values between SYSUP0004T and members of the genus Cellulomonas were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity.
32238231	12	45	theme	strain	1272:1277	arg1	SYSUP0004T					1279:1288	strain SYSUP0004T	1272:1288	strain SYSUP0004T	1272:1288	Thus, based on the above results strain SYSUP0004T represents a novel species of the genus Cellulomonas, for which the name Cellulomonas endophytica sp.
32238231	12	46	theme	endophytica	1376:1386	arg1	sp					1388:1389	the name Cellulomonas endophytica sp	1354:1389	the name Cellulomonas endophytica sp	1354:1389	Thus, based on the above results strain SYSUP0004T represents a novel species of the genus Cellulomonas, for which the name Cellulomonas endophytica sp.
32238231	5	47	theme	peptidoglycan	577:589	arg1	type					591:594	The cell-wall peptidoglycan type	563:594	The cell-wall peptidoglycan type	563:594	The cell-wall peptidoglycan type was A4β with an interpeptide bridge l-ornithine-d-glutamic acid.
32238231	3	48	theme	type	369:372	arg1	strain					374:379	the type strain	365:379	the type strain of Cellulomonas marina	365:402	The 16S rRNA gene sequence result showed that the strain SYSUP0004T shared low similarity (97.7 %) with the type strain of Cellulomonas marina.
32238231	2	49	theme	Gram-stain-positive	74:92	arg1	strain					134:139	A Gram-stain-positive, facultatively anaerobic and non-motile strain	72:139	A Gram-stain-positive, facultatively anaerobic and non-motile strain	72:139	A Gram-stain-positive, facultatively anaerobic and non-motile strain, designated SYSUP0004T, was isolated from the tubers of Gastrodia elata Blume collected from Yunnan Province, PR China.
32238231	5	50	theme	cell-wall	567:575	arg1	type					591:594	The cell-wall peptidoglycan type	563:594	The cell-wall peptidoglycan type	563:594	The cell-wall peptidoglycan type was A4β with an interpeptide bridge l-ornithine-d-glutamic acid.
32238231	2	51	theme	elata	207:211	arg1	Blume					213:217	Blume	213:217	Blume	213:217	A Gram-stain-positive, facultatively anaerobic and non-motile strain, designated SYSUP0004T, was isolated from the tubers of Gastrodia elata Blume collected from Yunnan Province, PR China.
32238231	2	51	theme	elata	207:211	arg1	tubers					187:192	the tubers	183:192	the tubers of Gastrodia elata Blume collected from Yunnan Province, PR China	183:258	A Gram-stain-positive, facultatively anaerobic and non-motile strain, designated SYSUP0004T, was isolated from the tubers of Gastrodia elata Blume collected from Yunnan Province, PR China.
32238231	4	52	dep	 w/v	524:527	arg1	%					523:523	%	523:523	%	523:523	SYSUP0004T grew at pH 6.0-9.0 (optimum, pH 8.0), temperature 4-30 °C (optimum, 28 °C) and could tolerate NaCl up to 4 % w/v (optimum in the absence of NaCl).
32238231	6	53	theme	Cell-wall	661:669	arg1	sugars					671:676	Cell-wall sugars	661:676	Cell-wall sugars	661:676	Cell-wall sugars were mannose, ribose, glucose, galactose and fucose.
32238231	11	54	theme	genus	1121:1125	arg1	Cellulomonas					1127:1138	the genus Cellulomonas	1117:1138	the genus Cellulomonas	1117:1138	The average nucleotide identity values between SYSUP0004T and members of the genus Cellulomonas were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity.
32238231	3	55	theme	gene	274:277	arg1	result					288:293	The 16S rRNA gene sequence result	261:293	The 16S rRNA gene sequence result	261:293	The 16S rRNA gene sequence result showed that the strain SYSUP0004T shared low similarity (97.7 %) with the type strain of Cellulomonas marina.
32238231	2	56	theme	Gastrodia	197:205	arg1	elata					207:211	Gastrodia elata	197:211	Gastrodia elata	197:211	A Gram-stain-positive, facultatively anaerobic and non-motile strain, designated SYSUP0004T, was isolated from the tubers of Gastrodia elata Blume collected from Yunnan Province, PR China.
32238231	9	57	theme	unidentified	979:990	arg1	glycolipid					992:1001	unidentified glycolipid	979:1001	unidentified glycolipid	979:1001	The polar lipids of SYSUP0004T were diphosphatidylglycerol, unidentified phosphoglycolipid, phosphatidylinositol mannosides and unidentified glycolipid.
32238231	12	58	theme	genus	1324:1328	arg1	Cellulomonas					1330:1341	the genus Cellulomonas	1320:1341	the genus Cellulomonas	1320:1341	Thus, based on the above results strain SYSUP0004T represents a novel species of the genus Cellulomonas, for which the name Cellulomonas endophytica sp.
32238231	3	59	theme	sequence	279:286	arg1	result					288:293	The 16S rRNA gene sequence result	261:293	The 16S rRNA gene sequence result	261:293	The 16S rRNA gene sequence result showed that the strain SYSUP0004T shared low similarity (97.7 %) with the type strain of Cellulomonas marina.
32238231	4	60	dep	%	523:523	arg1	4 					521:522	4 	521:522	4 	521:522	SYSUP0004T grew at pH 6.0-9.0 (optimum, pH 8.0), temperature 4-30 °C (optimum, 28 °C) and could tolerate NaCl up to 4 % w/v (optimum in the absence of NaCl).
32238231	11	61	theme	nucleotide	1056:1065	arg1	criterion					1202:1210	the ANI criterion	1194:1210	the ANI criterion for interspecies identity	1194:1236	The average nucleotide identity values between SYSUP0004T and members of the genus Cellulomonas were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity.
32238231	11	61	theme	nucleotide	1056:1065	arg1	values					1076:1081	The average nucleotide identity values	1044:1081	The average nucleotide identity values between SYSUP0004T and members of the genus Cellulomonas	1044:1138	The average nucleotide identity values between SYSUP0004T and members of the genus Cellulomonas were below the cut-off level (95-96 %) recommended as the ANI criterion for interspecies identity.
32238231	9	62	theme	polar	855:859	arg1	lipids					861:866	The polar lipids	851:866	The polar lipids of SYSUP0004T	851:880	The polar lipids of SYSUP0004T were diphosphatidylglycerol, unidentified phosphoglycolipid, phosphatidylinositol mannosides and unidentified glycolipid.
32238231	9	62	theme	polar	855:859	arg1	diphosphatidylglycerol					887:908	diphosphatidylglycerol	887:908	diphosphatidylglycerol	887:908	The polar lipids of SYSUP0004T were diphosphatidylglycerol, unidentified phosphoglycolipid, phosphatidylinositol mannosides and unidentified glycolipid.
32238231	9	63	theme	SYSUP0004T	871:880	arg1	lipids					861:866	The polar lipids	851:866	The polar lipids of SYSUP0004T	851:880	The polar lipids of SYSUP0004T were diphosphatidylglycerol, unidentified phosphoglycolipid, phosphatidylinositol mannosides and unidentified glycolipid.
32238231	9	63	theme	SYSUP0004T	871:880	arg1	diphosphatidylglycerol					887:908	diphosphatidylglycerol	887:908	diphosphatidylglycerol	887:908	The polar lipids of SYSUP0004T were diphosphatidylglycerol, unidentified phosphoglycolipid, phosphatidylinositol mannosides and unidentified glycolipid.
32238231	3	64	theme	strain	311:316	arg1	SYSUP0004T					318:327	the strain SYSUP0004T	307:327	the strain SYSUP0004T	307:327	The 16S rRNA gene sequence result showed that the strain SYSUP0004T shared low similarity (97.7 %) with the type strain of Cellulomonas marina.
32238231	4	65	dep	4 	521:522	arg1	to					518:519	to	518:519	to	518:519	SYSUP0004T grew at pH 6.0-9.0 (optimum, pH 8.0), temperature 4-30 °C (optimum, 28 °C) and could tolerate NaCl up to 4 % w/v (optimum in the absence of NaCl).
32238231	8	66	dep	A	819:819	arg1	anteiso-C17 					834:845	anteiso-C17 	834:845	anteiso-C17 	834:845	The major fatty acids were anteiso-C15:0, anteiso-C15 : 1 A, C16 : 0 and anteiso-C17 : 0.
32238231	8	66	dep	A	819:819	arg1	 0					827:828	 0	827:828	 0	827:828	The major fatty acids were anteiso-C15:0, anteiso-C15 : 1 A, C16 : 0 and anteiso-C17 : 0.
32238231	8	66	dep	A	819:819	arg1	 0					847:848	 0	847:848	 0	847:848	The major fatty acids were anteiso-C15:0, anteiso-C15 : 1 A, C16 : 0 and anteiso-C17 : 0.
32238231	14	67	theme	49025T=CGMCC	1445:1456	arg1	1.16405T					1458:1465	=KCTC 49025T=CGMCC 1.16405T	1439:1465	=KCTC 49025T=CGMCC 1.16405T	1439:1465	The type strain, SYSUP0004T (=KCTC 49025T=CGMCC 1.16405T).
32238231	14	67	theme	49025T=CGMCC	1445:1456	arg1	SYSUP0004T					1427:1436	SYSUP0004T	1427:1436	SYSUP0004T (=KCTC 49025T=CGMCC 1.16405T)	1427:1466	The type strain, SYSUP0004T (=KCTC 49025T=CGMCC 1.16405T).
32385312	0	0	theme	chemical	67:74	arg1	composition					76:86	cork chemical composition	62:86	cork chemical composition	62:86	Cork oak and climate change: Disentangling drought effects on cork chemical composition.
32385312	7	1	theme	chemical	1486:1493	arg1	composition					1495:1505	the chemical composition	1482:1505	the chemical composition of cork than the drought conditions occurring during cork growth	1482:1570	Therefore, our research suggests that the tree genetic information, or its expression, plays a much more important role on the chemical composition of cork than the drought conditions occurring during cork growth.
32385312	1	2	theme	frequent	145:152	arg1	region					133:138	the Mediterranean region	115:138	the Mediterranean region more frequent	115:152	Climate change induces in the Mediterranean region more frequent and extreme events, namely, heat waves and droughts, disturbing forest species and affecting their productivity and product quality.
32385312	5	3	theme	increased	1115:1123	arg1	extractives					1133:1143	drought increased ethanol extractives	1107:1143	drought increased ethanol extractives	1107:1143	The results show that, in general, drought does not affect the proportion of the main components of cork, the monomers of suberin or of polysaccharides, with few exceptions e.g. drought increased ethanol extractives and xylose in polysaccharides and decreased arabinose in polysaccharides.
32385312	3	4	dep	composition	601:611	arg1	polysaccharides					631:645	polysaccharides	631:645	polysaccharides	631:645	Since most cork properties arise from its chemical composition, this research studies the effect of drought on cork chemical composition (suberin, lignin, polysaccharides and extractives) and on polysaccharide and suberin monomeric composition.
32385312	3	4	dep	composition	601:611	arg1	lignin					623:628	lignin	623:628	lignin	623:628	Since most cork properties arise from its chemical composition, this research studies the effect of drought on cork chemical composition (suberin, lignin, polysaccharides and extractives) and on polysaccharide and suberin monomeric composition.
32385312	3	4	dep	composition	601:611	arg1	suberin					614:620	suberin	614:620	suberin	614:620	Since most cork properties arise from its chemical composition, this research studies the effect of drought on cork chemical composition (suberin, lignin, polysaccharides and extractives) and on polysaccharide and suberin monomeric composition.
32385312	3	4	dep	composition	601:611	arg1	extractives					651:661	extractives	651:661	extractives	651:661	Since most cork properties arise from its chemical composition, this research studies the effect of drought on cork chemical composition (suberin, lignin, polysaccharides and extractives) and on polysaccharide and suberin monomeric composition.
32385312	0	5	theme	cork	62:65	arg1	composition					76:86	cork chemical composition	62:86	cork chemical composition	62:86	Cork oak and climate change: Disentangling drought effects on cork chemical composition.
32385312	2	6	used	used	420:423	arg2	cork					385:388	cork	385:388	cork	385:388	The cork oak (Quercus suber) is present along the western Mediterranean basin and its outer bark (cork) is sustainably collected and used for several products, mainly wine bottle stoppers.
32385312	2	6	used	used	420:423	arg2	bark					379:382	its outer bark	369:382	its outer bark (cork)	369:389	The cork oak (Quercus suber) is present along the western Mediterranean basin and its outer bark (cork) is sustainably collected and used for several products, mainly wine bottle stoppers.
32385312	5	7	with	proportion	992:1001	arg1	exceptions					1091:1100	few exceptions	1087:1100	few exceptions e.g. drought increased ethanol extractives and xylose in polysaccharides and decreased arabinose in polysaccharides	1087:1216	The results show that, in general, drought does not affect the proportion of the main components of cork, the monomers of suberin or of polysaccharides, with few exceptions e.g. drought increased ethanol extractives and xylose in polysaccharides and decreased arabinose in polysaccharides.
32385312	7	8	theme	genetic	1406:1412	arg1	information					1414:1424	the tree genetic information	1397:1424	the tree genetic information	1397:1424	Therefore, our research suggests that the tree genetic information, or its expression, plays a much more important role on the chemical composition of cork than the drought conditions occurring during cork growth.
32385312	5	9	theme	ethanol	1125:1131	arg1	extractives					1133:1143	drought increased ethanol extractives	1107:1143	drought increased ethanol extractives	1107:1143	The results show that, in general, drought does not affect the proportion of the main components of cork, the monomers of suberin or of polysaccharides, with few exceptions e.g. drought increased ethanol extractives and xylose in polysaccharides and decreased arabinose in polysaccharides.
32385312	6	10	theme	drought	1299:1305	arg1	conditions					1307:1316	drought conditions	1299:1316	drought conditions	1299:1316	The variability associated to the tree is much more relevant than the effect of drought conditions and affects all the parameters analyzed.
32385312	5	11	theme	main	1010:1013	arg1	components					1015:1024	the main components	1006:1024	the main components of cork, the monomers of suberin or of polysaccharides,	1006:1080	The results show that, in general, drought does not affect the proportion of the main components of cork, the monomers of suberin or of polysaccharides, with few exceptions e.g. drought increased ethanol extractives and xylose in polysaccharides and decreased arabinose in polysaccharides.
32385312	3	12	theme	cork	487:490	arg1	properties					492:501	most cork properties	482:501	most cork properties	482:501	Since most cork properties arise from its chemical composition, this research studies the effect of drought on cork chemical composition (suberin, lignin, polysaccharides and extractives) and on polysaccharide and suberin monomeric composition.
32385312	5	13	theme	polysaccharides	1065:1079	arg1	cork					1029:1032	cork	1029:1032	cork	1029:1032	The results show that, in general, drought does not affect the proportion of the main components of cork, the monomers of suberin or of polysaccharides, with few exceptions e.g. drought increased ethanol extractives and xylose in polysaccharides and decreased arabinose in polysaccharides.
32385312	5	13	theme	polysaccharides	1065:1079	arg1	monomers					1039:1046	the monomers	1035:1046	the monomers of suberin or of polysaccharides	1035:1079	The results show that, in general, drought does not affect the proportion of the main components of cork, the monomers of suberin or of polysaccharides, with few exceptions e.g. drought increased ethanol extractives and xylose in polysaccharides and decreased arabinose in polysaccharides.
32385312	5	13	theme	polysaccharides	1065:1079	arg1	polysaccharides					1065:1079	polysaccharides	1065:1079	polysaccharides	1065:1079	The results show that, in general, drought does not affect the proportion of the main components of cork, the monomers of suberin or of polysaccharides, with few exceptions e.g. drought increased ethanol extractives and xylose in polysaccharides and decreased arabinose in polysaccharides.
32385312	5	13	theme	polysaccharides	1065:1079	arg1	suberin					1051:1057	suberin	1051:1057	suberin	1051:1057	The results show that, in general, drought does not affect the proportion of the main components of cork, the monomers of suberin or of polysaccharides, with few exceptions e.g. drought increased ethanol extractives and xylose in polysaccharides and decreased arabinose in polysaccharides.
32385312	4	14	theme	drought	841:847	arg1	events					849:854	another two drought events	829:854	another two drought events	829:854	Three sets of cork samples, from the same site, were examined: in one set the cork grew without drought; in another two drought events occurred during cork growth and in the third one drought event happened.
32385312	8	15	theme	wine	1731:1734	arg1	bottles					1736:1742	wine bottles	1731:1742	wine bottles	1731:1742	In practical terms, the potential increased occurrence of droughts arising from climatic changes will not compromise the performance of cork as a sealant for wine bottles.
32385312	5	16	from	extractives	1133:1143	arg1	arabinose					1189:1197	decreased arabinose	1179:1197	decreased arabinose in polysaccharides	1179:1216	The results show that, in general, drought does not affect the proportion of the main components of cork, the monomers of suberin or of polysaccharides, with few exceptions e.g. drought increased ethanol extractives and xylose in polysaccharides and decreased arabinose in polysaccharides.
32385312	5	16	from	extractives	1133:1143	arg1	polysaccharides					1159:1173	polysaccharides	1159:1173	polysaccharides	1159:1173	The results show that, in general, drought does not affect the proportion of the main components of cork, the monomers of suberin or of polysaccharides, with few exceptions e.g. drought increased ethanol extractives and xylose in polysaccharides and decreased arabinose in polysaccharides.
32385312	7	17	theme	cork	1560:1563	arg1	growth					1565:1570	cork growth	1560:1570	cork growth	1560:1570	Therefore, our research suggests that the tree genetic information, or its expression, plays a much more important role on the chemical composition of cork than the drought conditions occurring during cork growth.
32385312	2	18	theme	bottle	459:464	arg1	stoppers					466:473	wine bottle stoppers	454:473	wine bottle stoppers	454:473	The cork oak (Quercus suber) is present along the western Mediterranean basin and its outer bark (cork) is sustainably collected and used for several products, mainly wine bottle stoppers.
32385312	2	18	theme	bottle	459:464	arg1	products					437:444	several products	429:444	several products	429:444	The cork oak (Quercus suber) is present along the western Mediterranean basin and its outer bark (cork) is sustainably collected and used for several products, mainly wine bottle stoppers.
32385312	1	19	theme	extreme	158:164	arg1	events					166:171	extreme events	158:171	extreme events	158:171	Climate change induces in the Mediterranean region more frequent and extreme events, namely, heat waves and droughts, disturbing forest species and affecting their productivity and product quality.
32385312	2	20	theme	wine	454:457	arg1	stoppers					466:473	wine bottle stoppers	454:473	wine bottle stoppers	454:473	The cork oak (Quercus suber) is present along the western Mediterranean basin and its outer bark (cork) is sustainably collected and used for several products, mainly wine bottle stoppers.
32385312	2	20	theme	wine	454:457	arg1	products					437:444	several products	429:444	several products	429:444	The cork oak (Quercus suber) is present along the western Mediterranean basin and its outer bark (cork) is sustainably collected and used for several products, mainly wine bottle stoppers.
32385312	8	21	theme	cork	1709:1712	arg1	sealant					1719:1725	a sealant	1717:1725	a sealant for wine bottles	1717:1742	In practical terms, the potential increased occurrence of droughts arising from climatic changes will not compromise the performance of cork as a sealant for wine bottles.
32385312	8	21	theme	cork	1709:1712	arg1	performance					1694:1704	the performance	1690:1704	the performance of cork	1690:1712	In practical terms, the potential increased occurrence of droughts arising from climatic changes will not compromise the performance of cork as a sealant for wine bottles.
32385312	3	22	theme	most	482:485	arg1	properties					492:501	most cork properties	482:501	most cork properties	482:501	Since most cork properties arise from its chemical composition, this research studies the effect of drought on cork chemical composition (suberin, lignin, polysaccharides and extractives) and on polysaccharide and suberin monomeric composition.
32385312	3	23	from	effect	566:571	arg1	polysaccharide					671:684	polysaccharide	671:684	polysaccharide	671:684	Since most cork properties arise from its chemical composition, this research studies the effect of drought on cork chemical composition (suberin, lignin, polysaccharides and extractives) and on polysaccharide and suberin monomeric composition.
32385312	3	23	from	effect	566:571	arg1	composition					708:718	suberin monomeric composition	690:718	suberin monomeric composition	690:718	Since most cork properties arise from its chemical composition, this research studies the effect of drought on cork chemical composition (suberin, lignin, polysaccharides and extractives) and on polysaccharide and suberin monomeric composition.
32385312	3	23	from	effect	566:571	arg1	composition					601:611	cork chemical composition	587:611	cork chemical composition (suberin, lignin, polysaccharides and extractives)	587:662	Since most cork properties arise from its chemical composition, this research studies the effect of drought on cork chemical composition (suberin, lignin, polysaccharides and extractives) and on polysaccharide and suberin monomeric composition.
32385312	7	24	theme	important	1464:1472	arg1	role					1474:1477	a much more important role	1452:1477	a much more important role	1452:1477	Therefore, our research suggests that the tree genetic information, or its expression, plays a much more important role on the chemical composition of cork than the drought conditions occurring during cork growth.
32385312	2	25	theme	several	429:435	arg1	stoppers					466:473	wine bottle stoppers	454:473	wine bottle stoppers	454:473	The cork oak (Quercus suber) is present along the western Mediterranean basin and its outer bark (cork) is sustainably collected and used for several products, mainly wine bottle stoppers.
32385312	2	25	theme	several	429:435	arg1	products					437:444	several products	429:444	several products	429:444	The cork oak (Quercus suber) is present along the western Mediterranean basin and its outer bark (cork) is sustainably collected and used for several products, mainly wine bottle stoppers.
32385312	5	26	dep	extractives	1133:1143	arg1	e.g.					1102:1105	e.g.	1102:1105	e.g.	1102:1105	The results show that, in general, drought does not affect the proportion of the main components of cork, the monomers of suberin or of polysaccharides, with few exceptions e.g. drought increased ethanol extractives and xylose in polysaccharides and decreased arabinose in polysaccharides.
32385312	5	27	from	polysaccharides	1159:1173	arg1	polysaccharides					1202:1216	polysaccharides	1202:1216	polysaccharides	1202:1216	The results show that, in general, drought does not affect the proportion of the main components of cork, the monomers of suberin or of polysaccharides, with few exceptions e.g. drought increased ethanol extractives and xylose in polysaccharides and decreased arabinose in polysaccharides.
32385312	8	28	theme	practical	1576:1584	arg1	terms					1586:1590	practical terms	1576:1590	practical terms	1576:1590	In practical terms, the potential increased occurrence of droughts arising from climatic changes will not compromise the performance of cork as a sealant for wine bottles.
32385312	2	29	theme	Mediterranean	345:357	arg1	basin					359:363	the western Mediterranean basin	333:363	the western Mediterranean basin	333:363	The cork oak (Quercus suber) is present along the western Mediterranean basin and its outer bark (cork) is sustainably collected and used for several products, mainly wine bottle stoppers.
32385312	0	30	theme	Cork	0:3	arg1	oak					5:7	Cork oak	0:7	Cork oak	0:7	Cork oak and climate change: Disentangling drought effects on cork chemical composition.
32385312	3	31	theme	suberin	690:696	arg1	composition					708:718	suberin monomeric composition	690:718	suberin monomeric composition	690:718	Since most cork properties arise from its chemical composition, this research studies the effect of drought on cork chemical composition (suberin, lignin, polysaccharides and extractives) and on polysaccharide and suberin monomeric composition.
32385312	4	32	theme	drought	905:911	arg1	event					913:917	the third one drought event	891:917	the third one drought event	891:917	Three sets of cork samples, from the same site, were examined: in one set the cork grew without drought; in another two drought events occurred during cork growth and in the third one drought event happened.
32385312	2	33	theme	cork	291:294	arg1	suber					309:313	Quercus suber	301:313	Quercus suber	301:313	The cork oak (Quercus suber) is present along the western Mediterranean basin and its outer bark (cork) is sustainably collected and used for several products, mainly wine bottle stoppers.
32385312	2	33	theme	cork	291:294	arg1	present					319:325	present	319:325	present	319:325	The cork oak (Quercus suber) is present along the western Mediterranean basin and its outer bark (cork) is sustainably collected and used for several products, mainly wine bottle stoppers.
32385312	2	33	theme	cork	291:294	arg1	oak					296:298	The cork oak	287:298	The cork oak (Quercus suber)	287:314	The cork oak (Quercus suber) is present along the western Mediterranean basin and its outer bark (cork) is sustainably collected and used for several products, mainly wine bottle stoppers.
32385312	7	34	theme	drought	1524:1530	arg1	conditions					1532:1541	the drought conditions	1520:1541	the drought conditions occurring during cork growth	1520:1570	Therefore, our research suggests that the tree genetic information, or its expression, plays a much more important role on the chemical composition of cork than the drought conditions occurring during cork growth.
32385312	2	35	theme	western	337:343	arg1	basin					359:363	the western Mediterranean basin	333:363	the western Mediterranean basin	333:363	The cork oak (Quercus suber) is present along the western Mediterranean basin and its outer bark (cork) is sustainably collected and used for several products, mainly wine bottle stoppers.
32385312	0	36	theme	climate	13:19	arg1	change					21:26	climate change	13:26	climate change	13:26	Cork oak and climate change: Disentangling drought effects on cork chemical composition.
32385312	3	37	theme	monomeric	698:706	arg1	composition					708:718	suberin monomeric composition	690:718	suberin monomeric composition	690:718	Since most cork properties arise from its chemical composition, this research studies the effect of drought on cork chemical composition (suberin, lignin, polysaccharides and extractives) and on polysaccharide and suberin monomeric composition.
32385312	1	38	theme	Climate	89:95	arg1	change					97:102	Climate change	89:102	Climate change	89:102	Climate change induces in the Mediterranean region more frequent and extreme events, namely, heat waves and droughts, disturbing forest species and affecting their productivity and product quality.
32385312	5	39	theme	decreased	1179:1187	arg1	arabinose					1189:1197	decreased arabinose	1179:1197	decreased arabinose in polysaccharides	1179:1216	The results show that, in general, drought does not affect the proportion of the main components of cork, the monomers of suberin or of polysaccharides, with few exceptions e.g. drought increased ethanol extractives and xylose in polysaccharides and decreased arabinose in polysaccharides.
32385312	3	40	theme	chemical	518:525	arg1	composition					527:537	its chemical composition	514:537	its chemical composition	514:537	Since most cork properties arise from its chemical composition, this research studies the effect of drought on cork chemical composition (suberin, lignin, polysaccharides and extractives) and on polysaccharide and suberin monomeric composition.
32385312	1	41	theme	product	270:276	arg1	quality					278:284	product quality	270:284	product quality	270:284	Climate change induces in the Mediterranean region more frequent and extreme events, namely, heat waves and droughts, disturbing forest species and affecting their productivity and product quality.
32385312	3	42	theme	cork	587:590	arg1	composition					601:611	cork chemical composition	587:611	cork chemical composition (suberin, lignin, polysaccharides and extractives)	587:662	Since most cork properties arise from its chemical composition, this research studies the effect of drought on cork chemical composition (suberin, lignin, polysaccharides and extractives) and on polysaccharide and suberin monomeric composition.
32385312	5	43	from	xylose	1149:1154	arg1	arabinose					1189:1197	decreased arabinose	1179:1197	decreased arabinose in polysaccharides	1179:1216	The results show that, in general, drought does not affect the proportion of the main components of cork, the monomers of suberin or of polysaccharides, with few exceptions e.g. drought increased ethanol extractives and xylose in polysaccharides and decreased arabinose in polysaccharides.
32385312	5	43	from	xylose	1149:1154	arg1	polysaccharides					1159:1173	polysaccharides	1159:1173	polysaccharides	1159:1173	The results show that, in general, drought does not affect the proportion of the main components of cork, the monomers of suberin or of polysaccharides, with few exceptions e.g. drought increased ethanol extractives and xylose in polysaccharides and decreased arabinose in polysaccharides.
32385312	8	44	theme	increased	1607:1615	arg1	occurrence					1617:1626	the potential increased occurrence	1593:1626	the potential increased occurrence of droughts arising from climatic changes	1593:1668	In practical terms, the potential increased occurrence of droughts arising from climatic changes will not compromise the performance of cork as a sealant for wine bottles.
32385312	1	45	theme	heat	182:185	arg1	waves					187:191	heat waves	182:191	heat waves	182:191	Climate change induces in the Mediterranean region more frequent and extreme events, namely, heat waves and droughts, disturbing forest species and affecting their productivity and product quality.
32385312	4	46	theme	third	895:899	arg1	event					913:917	the third one drought event	891:917	the third one drought event	891:917	Three sets of cork samples, from the same site, were examined: in one set the cork grew without drought; in another two drought events occurred during cork growth and in the third one drought event happened.
32385312	5	47	theme	suberin	1051:1057	arg1	cork					1029:1032	cork	1029:1032	cork	1029:1032	The results show that, in general, drought does not affect the proportion of the main components of cork, the monomers of suberin or of polysaccharides, with few exceptions e.g. drought increased ethanol extractives and xylose in polysaccharides and decreased arabinose in polysaccharides.
32385312	5	47	theme	suberin	1051:1057	arg1	monomers					1039:1046	the monomers	1035:1046	the monomers of suberin or of polysaccharides	1035:1079	The results show that, in general, drought does not affect the proportion of the main components of cork, the monomers of suberin or of polysaccharides, with few exceptions e.g. drought increased ethanol extractives and xylose in polysaccharides and decreased arabinose in polysaccharides.
32385312	5	47	theme	suberin	1051:1057	arg1	polysaccharides					1065:1079	polysaccharides	1065:1079	polysaccharides	1065:1079	The results show that, in general, drought does not affect the proportion of the main components of cork, the monomers of suberin or of polysaccharides, with few exceptions e.g. drought increased ethanol extractives and xylose in polysaccharides and decreased arabinose in polysaccharides.
32385312	5	47	theme	suberin	1051:1057	arg1	suberin					1051:1057	suberin	1051:1057	suberin	1051:1057	The results show that, in general, drought does not affect the proportion of the main components of cork, the monomers of suberin or of polysaccharides, with few exceptions e.g. drought increased ethanol extractives and xylose in polysaccharides and decreased arabinose in polysaccharides.
32385312	7	48	theme	cork	1510:1513	arg1	composition					1495:1505	the chemical composition	1482:1505	the chemical composition of cork than the drought conditions occurring during cork growth	1482:1570	Therefore, our research suggests that the tree genetic information, or its expression, plays a much more important role on the chemical composition of cork than the drought conditions occurring during cork growth.
32385312	3	49	theme	chemical	592:599	arg1	composition					601:611	cork chemical composition	587:611	cork chemical composition (suberin, lignin, polysaccharides and extractives)	587:662	Since most cork properties arise from its chemical composition, this research studies the effect of drought on cork chemical composition (suberin, lignin, polysaccharides and extractives) and on polysaccharide and suberin monomeric composition.
32385312	5	50	from	arabinose	1189:1197	arg1	polysaccharides					1202:1216	polysaccharides	1202:1216	polysaccharides	1202:1216	The results show that, in general, drought does not affect the proportion of the main components of cork, the monomers of suberin or of polysaccharides, with few exceptions e.g. drought increased ethanol extractives and xylose in polysaccharides and decreased arabinose in polysaccharides.
32385312	8	51	theme	potential	1597:1605	arg1	occurrence					1617:1626	the potential increased occurrence	1593:1626	the potential increased occurrence of droughts arising from climatic changes	1593:1668	In practical terms, the potential increased occurrence of droughts arising from climatic changes will not compromise the performance of cork as a sealant for wine bottles.
32385312	2	52	theme	outer	373:377	arg1	cork					385:388	cork	385:388	cork	385:388	The cork oak (Quercus suber) is present along the western Mediterranean basin and its outer bark (cork) is sustainably collected and used for several products, mainly wine bottle stoppers.
32385312	2	52	theme	outer	373:377	arg1	bark					379:382	its outer bark	369:382	its outer bark (cork)	369:389	The cork oak (Quercus suber) is present along the western Mediterranean basin and its outer bark (cork) is sustainably collected and used for several products, mainly wine bottle stoppers.
32385312	5	53	theme	components	1015:1024	arg1	proportion					992:1001	the proportion	988:1001	the proportion of the main components of cork, the monomers of suberin or of polysaccharides, with few exceptions e.g. drought increased ethanol extractives and xylose in polysaccharides and decreased arabinose in polysaccharides	988:1216	The results show that, in general, drought does not affect the proportion of the main components of cork, the monomers of suberin or of polysaccharides, with few exceptions e.g. drought increased ethanol extractives and xylose in polysaccharides and decreased arabinose in polysaccharides.
32385312	3	54	theme	drought	576:582	arg1	effect					566:571	the effect	562:571	the effect of drought on cork chemical composition (suberin, lignin, polysaccharides and extractives) and on polysaccharide and suberin monomeric composition	562:718	Since most cork properties arise from its chemical composition, this research studies the effect of drought on cork chemical composition (suberin, lignin, polysaccharides and extractives) and on polysaccharide and suberin monomeric composition.
32385312	6	55	theme	conditions	1307:1316	arg1	effect					1289:1294	the effect	1285:1294	the effect of drought conditions	1285:1316	The variability associated to the tree is much more relevant than the effect of drought conditions and affects all the parameters analyzed.
32385312	4	56	from	site	763:766	arg1	samples					740:746	cork samples	735:746	cork samples	735:746	Three sets of cork samples, from the same site, were examined: in one set the cork grew without drought; in another two drought events occurred during cork growth and in the third one drought event happened.
32385312	4	56	from	site	763:766	arg1	sets					727:730	Three sets	721:730	Three sets	721:730	Three sets of cork samples, from the same site, were examined: in one set the cork grew without drought; in another two drought events occurred during cork growth and in the third one drought event happened.
32385312	0	57	theme	drought	43:49	arg1	effects					51:57	Disentangling drought effects	29:57	Cork oak and climate change: Disentangling drought effects on cork chemical composition.	0:87	Cork oak and climate change: Disentangling drought effects on cork chemical composition.
32385312	7	58	theme	more	1459:1462	arg1	role					1474:1477	a much more important role	1452:1477	a much more important role	1452:1477	Therefore, our research suggests that the tree genetic information, or its expression, plays a much more important role on the chemical composition of cork than the drought conditions occurring during cork growth.
32385312	0	59	from	effects	51:57	arg1	composition					76:86	cork chemical composition	62:86	cork chemical composition	62:86	Cork oak and climate change: Disentangling drought effects on cork chemical composition.
32385312	0	60	theme	Disentangling	29:41	arg1	effects					51:57	Disentangling drought effects	29:57	Cork oak and climate change: Disentangling drought effects on cork chemical composition.	0:87	Cork oak and climate change: Disentangling drought effects on cork chemical composition.
32385312	5	61	theme	cork	1029:1032	arg1	components					1015:1024	the main components	1006:1024	the main components of cork, the monomers of suberin or of polysaccharides,	1006:1080	The results show that, in general, drought does not affect the proportion of the main components of cork, the monomers of suberin or of polysaccharides, with few exceptions e.g. drought increased ethanol extractives and xylose in polysaccharides and decreased arabinose in polysaccharides.
32385312	4	62	theme	same	758:761	arg1	site					763:766	the same site	754:766	the same site	754:766	Three sets of cork samples, from the same site, were examined: in one set the cork grew without drought; in another two drought events occurred during cork growth and in the third one drought event happened.
32385312	7	63	theme	tree	1401:1404	arg1	information					1414:1424	the tree genetic information	1397:1424	the tree genetic information	1397:1424	Therefore, our research suggests that the tree genetic information, or its expression, plays a much more important role on the chemical composition of cork than the drought conditions occurring during cork growth.
32385312	4	64	theme	cork	735:738	arg1	samples					740:746	cork samples	735:746	cork samples	735:746	Three sets of cork samples, from the same site, were examined: in one set the cork grew without drought; in another two drought events occurred during cork growth and in the third one drought event happened.
32385312	8	65	theme	droughts	1631:1638	arg1	occurrence					1617:1626	the potential increased occurrence	1593:1626	the potential increased occurrence of droughts arising from climatic changes	1593:1668	In practical terms, the potential increased occurrence of droughts arising from climatic changes will not compromise the performance of cork as a sealant for wine bottles.
32385312	2	66	theme	Quercus	301:307	arg1	suber					309:313	Quercus suber	301:313	Quercus suber	301:313	The cork oak (Quercus suber) is present along the western Mediterranean basin and its outer bark (cork) is sustainably collected and used for several products, mainly wine bottle stoppers.
32385312	2	66	theme	Quercus	301:307	arg1	oak					296:298	The cork oak	287:298	The cork oak (Quercus suber)	287:314	The cork oak (Quercus suber) is present along the western Mediterranean basin and its outer bark (cork) is sustainably collected and used for several products, mainly wine bottle stoppers.
32385312	8	67	theme	climatic	1653:1660	arg1	changes					1662:1668	climatic changes	1653:1668	climatic changes	1653:1668	In practical terms, the potential increased occurrence of droughts arising from climatic changes will not compromise the performance of cork as a sealant for wine bottles.
32385312	1	68	theme	Mediterranean	119:131	arg1	region					133:138	the Mediterranean region	115:138	the Mediterranean region more frequent	115:152	Climate change induces in the Mediterranean region more frequent and extreme events, namely, heat waves and droughts, disturbing forest species and affecting their productivity and product quality.
32385312	5	69	theme	few	1087:1089	arg1	exceptions					1091:1100	few exceptions	1087:1100	few exceptions e.g. drought increased ethanol extractives and xylose in polysaccharides and decreased arabinose in polysaccharides	1087:1216	The results show that, in general, drought does not affect the proportion of the main components of cork, the monomers of suberin or of polysaccharides, with few exceptions e.g. drought increased ethanol extractives and xylose in polysaccharides and decreased arabinose in polysaccharides.
32385312	4	70	dep	drought	817:823	arg1	occurred					856:863	occurred	856:863	occurred during cork growth	856:882	Three sets of cork samples, from the same site, were examined: in one set the cork grew without drought; in another two drought events occurred during cork growth and in the third one drought event happened.
32385312	4	70	dep	drought	817:823	arg1	happened					919:926	happened	919:926	happened	919:926	Three sets of cork samples, from the same site, were examined: in one set the cork grew without drought; in another two drought events occurred during cork growth and in the third one drought event happened.
32385312	0	71	dep	oak	5:7	arg1	effects					51:57	Disentangling drought effects	29:57	Cork oak and climate change: Disentangling drought effects on cork chemical composition.	0:87	Cork oak and climate change: Disentangling drought effects on cork chemical composition.
32385312	4	72	theme	cork	872:875	arg1	growth					877:882	cork growth	872:882	cork growth	872:882	Three sets of cork samples, from the same site, were examined: in one set the cork grew without drought; in another two drought events occurred during cork growth and in the third one drought event happened.
32385312	4	73	theme	samples	740:746	arg1	samples					740:746	cork samples	735:746	cork samples	735:746	Three sets of cork samples, from the same site, were examined: in one set the cork grew without drought; in another two drought events occurred during cork growth and in the third one drought event happened.
32385312	4	73	theme	samples	740:746	arg1	sets					727:730	Three sets	721:730	Three sets	721:730	Three sets of cork samples, from the same site, were examined: in one set the cork grew without drought; in another two drought events occurred during cork growth and in the third one drought event happened.
32385312	5	74	theme	drought	1107:1113	arg1	extractives					1133:1143	drought increased ethanol extractives	1107:1143	drought increased ethanol extractives	1107:1143	The results show that, in general, drought does not affect the proportion of the main components of cork, the monomers of suberin or of polysaccharides, with few exceptions e.g. drought increased ethanol extractives and xylose in polysaccharides and decreased arabinose in polysaccharides.
32385312	1	75	theme	forest	218:223	arg1	species					225:231	forest species	218:231	forest species	218:231	Climate change induces in the Mediterranean region more frequent and extreme events, namely, heat waves and droughts, disturbing forest species and affecting their productivity and product quality.
31953507	7	0	theme	Carbohydrate	1306:1317	arg1	genes					1336:1340	Carbohydrate depolymerization genes	1306:1340	Carbohydrate depolymerization genes	1306:1340	Carbohydrate depolymerization genes were consistently upregulated in the rhizosphere, and both taxonomic and functional diversity were highest in the combined rhizosphere-detritusphere, suggesting coexistence of rhizosphere guilds is facilitated by niche differentiation.
31953507	6	1	theme	functional	1105:1114	arg1	patterns					1127:1134	patterns	1127:1134	patterns	1127:1134	Using hierarchical clustering of upregulated decomposition genes, we identified four distinct microbial guilds populated by taxa whose functional succession patterns suggest specialization for substrates provided by fresh growing roots, decaying root detritus, the combination of live and decaying root biomass, or aging root material.
31953507	4	2	theme	isotope	745:751	arg1	metagenomes					761:771	stable isotope probing metagenomes	738:771	stable isotope probing metagenomes	738:771	Transcripts were binned using a unique reference database generated from soil isolate genomes, single-cell amplified genomes, metagenomes, and stable isotope probing metagenomes.
31953507	7	3	theme	depolymerization	1319:1334	arg1	genes					1336:1340	Carbohydrate depolymerization genes	1306:1340	Carbohydrate depolymerization genes	1306:1340	Carbohydrate depolymerization genes were consistently upregulated in the rhizosphere, and both taxonomic and functional diversity were highest in the combined rhizosphere-detritusphere, suggesting coexistence of rhizosphere guilds is facilitated by niche differentiation.
31953507	8	4	theme	soil	1688:1691	arg1	cycling					1700:1706	soil carbon cycling	1688:1706	soil carbon cycling	1688:1706	Metatranscriptome-defined guilds provide a framework to model rhizosphere succession and its consequences for soil carbon cycling.
31953507	2	5	theme	carbohydrate	344:355	arg1	depolymerization					357:372	rhizosphere carbohydrate depolymerization	332:372	rhizosphere carbohydrate depolymerization	332:372	However, the ecological mechanisms that underpin rhizosphere carbohydrate depolymerization are poorly understood.
31953507	1	6	theme	soil	256:259	arg1	SOM					277:279	SOM	277:279	SOM	277:279	The rhizosphere is a hotspot for microbial carbon transformations, and is the entry point for root polysaccharides and polymeric carbohydrates that are important precursors to soil organic matter (SOM).
31953507	1	6	theme	soil	256:259	arg1	matter					269:274	soil organic matter	256:274	soil organic matter (SOM)	256:280	The rhizosphere is a hotspot for microbial carbon transformations, and is the entry point for root polysaccharides and polymeric carbohydrates that are important precursors to soil organic matter (SOM).
31953507	5	7	theme	soil	780:783	arg1	habitat					785:791	soil habitat	780:791	soil habitat	780:791	While soil habitat significantly affected both community composition and overall gene expression, the succession of microbial functions occurred at a faster time scale than compositional changes.
31953507	6	8	theme	hierarchical	976:987	arg1	clustering					989:998	hierarchical clustering	976:998	hierarchical clustering of upregulated decomposition genes	976:1033	Using hierarchical clustering of upregulated decomposition genes, we identified four distinct microbial guilds populated by taxa whose functional succession patterns suggest specialization for substrates provided by fresh growing roots, decaying root detritus, the combination of live and decaying root biomass, or aging root material.
31953507	3	9	theme	rhizosphere-detritusphere	559:583	arg1	habitats					585:592	combined rhizosphere-detritusphere habitats	550:592	combined rhizosphere-detritusphere habitats	550:592	Using Avena fatua, a common annual grass, we analyzed time-resolved metatranscriptomes to compare microbial functions in rhizosphere, detritusphere, and combined rhizosphere-detritusphere habitats.
31953507	1	10	theme	organic	261:267	arg1	SOM					277:279	SOM	277:279	SOM	277:279	The rhizosphere is a hotspot for microbial carbon transformations, and is the entry point for root polysaccharides and polymeric carbohydrates that are important precursors to soil organic matter (SOM).
31953507	1	10	theme	organic	261:267	arg1	matter					269:274	soil organic matter	256:274	soil organic matter (SOM)	256:280	The rhizosphere is a hotspot for microbial carbon transformations, and is the entry point for root polysaccharides and polymeric carbohydrates that are important precursors to soil organic matter (SOM).
31953507	6	11	theme	decaying	1259:1266	arg1	biomass					1273:1279	live and decaying root biomass	1250:1279	live and decaying root biomass	1250:1279	Using hierarchical clustering of upregulated decomposition genes, we identified four distinct microbial guilds populated by taxa whose functional succession patterns suggest specialization for substrates provided by fresh growing roots, decaying root detritus, the combination of live and decaying root biomass, or aging root material.
31953507	0	12	theme	Niche	0:4	arg1	differentiation					6:20	Niche differentiation	0:20	Niche differentiation	0:20	Niche differentiation is spatially and temporally regulated in the rhizosphere.
31953507	4	13	theme	unique	627:632	arg1	database					644:651	a unique reference database	625:651	a unique reference database generated from soil isolate genomes, single-cell amplified genomes, metagenomes, and stable isotope probing metagenomes	625:771	Transcripts were binned using a unique reference database generated from soil isolate genomes, single-cell amplified genomes, metagenomes, and stable isotope probing metagenomes.
31953507	8	14	theme	carbon	1693:1698	arg1	cycling					1700:1706	soil carbon cycling	1688:1706	soil carbon cycling	1688:1706	Metatranscriptome-defined guilds provide a framework to model rhizosphere succession and its consequences for soil carbon cycling.
31953507	6	15	theme	growing	1192:1198	arg1	roots					1200:1204	fresh growing roots	1186:1204	fresh growing roots	1186:1204	Using hierarchical clustering of upregulated decomposition genes, we identified four distinct microbial guilds populated by taxa whose functional succession patterns suggest specialization for substrates provided by fresh growing roots, decaying root detritus, the combination of live and decaying root biomass, or aging root material.
31953507	6	16	theme	biomass	1273:1279	arg1	material					1296:1303	aging root material	1285:1303	aging root material	1285:1303	Using hierarchical clustering of upregulated decomposition genes, we identified four distinct microbial guilds populated by taxa whose functional succession patterns suggest specialization for substrates provided by fresh growing roots, decaying root detritus, the combination of live and decaying root biomass, or aging root material.
31953507	6	16	theme	biomass	1273:1279	arg1	detritus					1221:1228	root detritus	1216:1228	root detritus	1216:1228	Using hierarchical clustering of upregulated decomposition genes, we identified four distinct microbial guilds populated by taxa whose functional succession patterns suggest specialization for substrates provided by fresh growing roots, decaying root detritus, the combination of live and decaying root biomass, or aging root material.
31953507	6	16	theme	biomass	1273:1279	arg1	combination					1235:1245	the combination	1231:1245	the combination of live and decaying root biomass	1231:1279	Using hierarchical clustering of upregulated decomposition genes, we identified four distinct microbial guilds populated by taxa whose functional succession patterns suggest specialization for substrates provided by fresh growing roots, decaying root detritus, the combination of live and decaying root biomass, or aging root material.
31953507	7	17	theme	combined	1456:1463	arg1	rhizosphere-detritusphere					1465:1489	the combined rhizosphere-detritusphere	1452:1489	the combined rhizosphere-detritusphere	1452:1489	Carbohydrate depolymerization genes were consistently upregulated in the rhizosphere, and both taxonomic and functional diversity were highest in the combined rhizosphere-detritusphere, suggesting coexistence of rhizosphere guilds is facilitated by niche differentiation.
31953507	1	18	theme	entry	158:162	arg1	point					164:168	the entry point	154:168	the entry point for root polysaccharides and polymeric carbohydrates that are important precursors to soil organic matter (SOM)	154:280	The rhizosphere is a hotspot for microbial carbon transformations, and is the entry point for root polysaccharides and polymeric carbohydrates that are important precursors to soil organic matter (SOM).
31953507	3	19	theme	combined	550:557	arg1	habitats					585:592	combined rhizosphere-detritusphere habitats	550:592	combined rhizosphere-detritusphere habitats	550:592	Using Avena fatua, a common annual grass, we analyzed time-resolved metatranscriptomes to compare microbial functions in rhizosphere, detritusphere, and combined rhizosphere-detritusphere habitats.
31953507	4	20	theme	amplified	702:710	arg1	genomes					712:718	single-cell amplified genomes	690:718	single-cell amplified genomes	690:718	Transcripts were binned using a unique reference database generated from soil isolate genomes, single-cell amplified genomes, metagenomes, and stable isotope probing metagenomes.
31953507	2	21	theme	ecological	296:305	arg1	mechanisms					307:316	the ecological mechanisms	292:316	the ecological mechanisms that underpin rhizosphere carbohydrate depolymerization	292:372	However, the ecological mechanisms that underpin rhizosphere carbohydrate depolymerization are poorly understood.
31953507	3	22	theme	annual	425:430	arg1	grass					432:436	a common annual grass	416:436	a common annual grass	416:436	Using Avena fatua, a common annual grass, we analyzed time-resolved metatranscriptomes to compare microbial functions in rhizosphere, detritusphere, and combined rhizosphere-detritusphere habitats.
31953507	3	22	theme	annual	425:430	arg1	fatua					409:413	Avena fatua	403:413	Avena fatua	403:413	Using Avena fatua, a common annual grass, we analyzed time-resolved metatranscriptomes to compare microbial functions in rhizosphere, detritusphere, and combined rhizosphere-detritusphere habitats.
31953507	3	23	theme	Avena	403:407	arg1	grass					432:436	a common annual grass	416:436	a common annual grass	416:436	Using Avena fatua, a common annual grass, we analyzed time-resolved metatranscriptomes to compare microbial functions in rhizosphere, detritusphere, and combined rhizosphere-detritusphere habitats.
31953507	3	23	theme	Avena	403:407	arg1	fatua					409:413	Avena fatua	403:413	Avena fatua	403:413	Using Avena fatua, a common annual grass, we analyzed time-resolved metatranscriptomes to compare microbial functions in rhizosphere, detritusphere, and combined rhizosphere-detritusphere habitats.
31953507	6	24	theme	microbial	1064:1072	arg1	guilds					1074:1079	four distinct microbial guilds	1050:1079	four distinct microbial guilds populated by taxa whose functional succession patterns suggest specialization for substrates provided by fresh growing roots, decaying root detritus, the combination of live and decaying root biomass, or aging root material	1050:1303	Using hierarchical clustering of upregulated decomposition genes, we identified four distinct microbial guilds populated by taxa whose functional succession patterns suggest specialization for substrates provided by fresh growing roots, decaying root detritus, the combination of live and decaying root biomass, or aging root material.
31953507	3	25	from	functions	505:513	arg1	habitats					585:592	combined rhizosphere-detritusphere habitats	550:592	combined rhizosphere-detritusphere habitats	550:592	Using Avena fatua, a common annual grass, we analyzed time-resolved metatranscriptomes to compare microbial functions in rhizosphere, detritusphere, and combined rhizosphere-detritusphere habitats.
31953507	3	25	from	functions	505:513	arg1	detritusphere					531:543	detritusphere	531:543	detritusphere	531:543	Using Avena fatua, a common annual grass, we analyzed time-resolved metatranscriptomes to compare microbial functions in rhizosphere, detritusphere, and combined rhizosphere-detritusphere habitats.
31953507	3	25	from	functions	505:513	arg1	rhizosphere					518:528	rhizosphere	518:528	rhizosphere	518:528	Using Avena fatua, a common annual grass, we analyzed time-resolved metatranscriptomes to compare microbial functions in rhizosphere, detritusphere, and combined rhizosphere-detritusphere habitats.
31953507	7	26	theme	niche	1555:1559	arg1	differentiation					1561:1575	niche differentiation	1555:1575	niche differentiation	1555:1575	Carbohydrate depolymerization genes were consistently upregulated in the rhizosphere, and both taxonomic and functional diversity were highest in the combined rhizosphere-detritusphere, suggesting coexistence of rhizosphere guilds is facilitated by niche differentiation.
31953507	1	27	theme	root	174:177	arg1	precursors					242:251	important precursors	232:251	important precursors to soil organic matter (SOM)	232:280	The rhizosphere is a hotspot for microbial carbon transformations, and is the entry point for root polysaccharides and polymeric carbohydrates that are important precursors to soil organic matter (SOM).
31953507	1	27	theme	root	174:177	arg1	polysaccharides					179:193	root polysaccharides	174:193	root polysaccharides	174:193	The rhizosphere is a hotspot for microbial carbon transformations, and is the entry point for root polysaccharides and polymeric carbohydrates that are important precursors to soil organic matter (SOM).
31953507	1	27	theme	root	174:177	arg1	carbohydrates					209:221	polymeric carbohydrates	199:221	polymeric carbohydrates	199:221	The rhizosphere is a hotspot for microbial carbon transformations, and is the entry point for root polysaccharides and polymeric carbohydrates that are important precursors to soil organic matter (SOM).
31953507	6	28	theme	distinct	1055:1062	arg1	guilds					1074:1079	four distinct microbial guilds	1050:1079	four distinct microbial guilds populated by taxa whose functional succession patterns suggest specialization for substrates provided by fresh growing roots, decaying root detritus, the combination of live and decaying root biomass, or aging root material	1050:1303	Using hierarchical clustering of upregulated decomposition genes, we identified four distinct microbial guilds populated by taxa whose functional succession patterns suggest specialization for substrates provided by fresh growing roots, decaying root detritus, the combination of live and decaying root biomass, or aging root material.
31953507	6	29	theme	live	1250:1253	arg1	biomass					1273:1279	live and decaying root biomass	1250:1279	live and decaying root biomass	1250:1279	Using hierarchical clustering of upregulated decomposition genes, we identified four distinct microbial guilds populated by taxa whose functional succession patterns suggest specialization for substrates provided by fresh growing roots, decaying root detritus, the combination of live and decaying root biomass, or aging root material.
31953507	6	30	theme	fresh	1186:1190	arg1	roots					1200:1204	fresh growing roots	1186:1204	fresh growing roots	1186:1204	Using hierarchical clustering of upregulated decomposition genes, we identified four distinct microbial guilds populated by taxa whose functional succession patterns suggest specialization for substrates provided by fresh growing roots, decaying root detritus, the combination of live and decaying root biomass, or aging root material.
31953507	5	31	theme	functions	900:908	arg1	succession					876:885	the succession	872:885	the succession of microbial functions	872:908	While soil habitat significantly affected both community composition and overall gene expression, the succession of microbial functions occurred at a faster time scale than compositional changes.
31953507	5	32	theme	microbial	890:898	arg1	functions					900:908	microbial functions	890:908	microbial functions	890:908	While soil habitat significantly affected both community composition and overall gene expression, the succession of microbial functions occurred at a faster time scale than compositional changes.
31953507	4	33	theme	stable	738:743	arg1	metagenomes					761:771	stable isotope probing metagenomes	738:771	stable isotope probing metagenomes	738:771	Transcripts were binned using a unique reference database generated from soil isolate genomes, single-cell amplified genomes, metagenomes, and stable isotope probing metagenomes.
31953507	4	34	theme	isolate	673:679	arg1	genomes					681:687	soil isolate genomes	668:687	soil isolate genomes	668:687	Transcripts were binned using a unique reference database generated from soil isolate genomes, single-cell amplified genomes, metagenomes, and stable isotope probing metagenomes.
31953507	5	35	theme	overall	847:853	arg1	expression					860:869	overall gene expression	847:869	overall gene expression	847:869	While soil habitat significantly affected both community composition and overall gene expression, the succession of microbial functions occurred at a faster time scale than compositional changes.
31953507	4	36	theme	probing	753:759	arg1	metagenomes					761:771	stable isotope probing metagenomes	738:771	stable isotope probing metagenomes	738:771	Transcripts were binned using a unique reference database generated from soil isolate genomes, single-cell amplified genomes, metagenomes, and stable isotope probing metagenomes.
31953507	4	37	theme	single-cell	690:700	arg1	genomes					712:718	single-cell amplified genomes	690:718	single-cell amplified genomes	690:718	Transcripts were binned using a unique reference database generated from soil isolate genomes, single-cell amplified genomes, metagenomes, and stable isotope probing metagenomes.
31953507	7	38	theme	rhizosphere	1518:1528	arg1	guilds					1530:1535	rhizosphere guilds	1518:1535	rhizosphere guilds	1518:1535	Carbohydrate depolymerization genes were consistently upregulated in the rhizosphere, and both taxonomic and functional diversity were highest in the combined rhizosphere-detritusphere, suggesting coexistence of rhizosphere guilds is facilitated by niche differentiation.
31953507	1	39	theme	polymeric	199:207	arg1	precursors					242:251	important precursors	232:251	important precursors to soil organic matter (SOM)	232:280	The rhizosphere is a hotspot for microbial carbon transformations, and is the entry point for root polysaccharides and polymeric carbohydrates that are important precursors to soil organic matter (SOM).
31953507	1	39	theme	polymeric	199:207	arg1	polysaccharides					179:193	root polysaccharides	174:193	root polysaccharides	174:193	The rhizosphere is a hotspot for microbial carbon transformations, and is the entry point for root polysaccharides and polymeric carbohydrates that are important precursors to soil organic matter (SOM).
31953507	1	39	theme	polymeric	199:207	arg1	carbohydrates					209:221	polymeric carbohydrates	199:221	polymeric carbohydrates	199:221	The rhizosphere is a hotspot for microbial carbon transformations, and is the entry point for root polysaccharides and polymeric carbohydrates that are important precursors to soil organic matter (SOM).
31953507	3	40	theme	common	418:423	arg1	grass					432:436	a common annual grass	416:436	a common annual grass	416:436	Using Avena fatua, a common annual grass, we analyzed time-resolved metatranscriptomes to compare microbial functions in rhizosphere, detritusphere, and combined rhizosphere-detritusphere habitats.
31953507	3	40	theme	common	418:423	arg1	fatua					409:413	Avena fatua	403:413	Avena fatua	403:413	Using Avena fatua, a common annual grass, we analyzed time-resolved metatranscriptomes to compare microbial functions in rhizosphere, detritusphere, and combined rhizosphere-detritusphere habitats.
31953507	5	41	theme	gene	855:858	arg1	expression					860:869	overall gene expression	847:869	overall gene expression	847:869	While soil habitat significantly affected both community composition and overall gene expression, the succession of microbial functions occurred at a faster time scale than compositional changes.
31953507	1	42	theme	important	232:240	arg1	precursors					242:251	important precursors	232:251	important precursors to soil organic matter (SOM)	232:280	The rhizosphere is a hotspot for microbial carbon transformations, and is the entry point for root polysaccharides and polymeric carbohydrates that are important precursors to soil organic matter (SOM).
31953507	1	42	theme	important	232:240	arg1	polysaccharides					179:193	root polysaccharides	174:193	root polysaccharides	174:193	The rhizosphere is a hotspot for microbial carbon transformations, and is the entry point for root polysaccharides and polymeric carbohydrates that are important precursors to soil organic matter (SOM).
31953507	1	42	theme	important	232:240	arg1	carbohydrates					209:221	polymeric carbohydrates	199:221	polymeric carbohydrates	199:221	The rhizosphere is a hotspot for microbial carbon transformations, and is the entry point for root polysaccharides and polymeric carbohydrates that are important precursors to soil organic matter (SOM).
31953507	3	43	theme	time-resolved	451:463	arg1	metatranscriptomes					465:482	time-resolved metatranscriptomes	451:482	time-resolved metatranscriptomes	451:482	Using Avena fatua, a common annual grass, we analyzed time-resolved metatranscriptomes to compare microbial functions in rhizosphere, detritusphere, and combined rhizosphere-detritusphere habitats.
31953507	5	44	theme	community	821:829	arg1	composition					831:841	community composition	821:841	community composition	821:841	While soil habitat significantly affected both community composition and overall gene expression, the succession of microbial functions occurred at a faster time scale than compositional changes.
31953507	6	45	theme	root	1216:1219	arg1	detritus					1221:1228	root detritus	1216:1228	root detritus	1216:1228	Using hierarchical clustering of upregulated decomposition genes, we identified four distinct microbial guilds populated by taxa whose functional succession patterns suggest specialization for substrates provided by fresh growing roots, decaying root detritus, the combination of live and decaying root biomass, or aging root material.
31953507	2	46	theme	rhizosphere	332:342	arg1	depolymerization					357:372	rhizosphere carbohydrate depolymerization	332:372	rhizosphere carbohydrate depolymerization	332:372	However, the ecological mechanisms that underpin rhizosphere carbohydrate depolymerization are poorly understood.
31953507	6	47	theme	genes	1029:1033	arg1	clustering					989:998	hierarchical clustering	976:998	hierarchical clustering of upregulated decomposition genes	976:1033	Using hierarchical clustering of upregulated decomposition genes, we identified four distinct microbial guilds populated by taxa whose functional succession patterns suggest specialization for substrates provided by fresh growing roots, decaying root detritus, the combination of live and decaying root biomass, or aging root material.
31953507	8	48	theme	rhizosphere	1640:1650	arg1	succession					1652:1661	model rhizosphere succession	1634:1661	model rhizosphere succession	1634:1661	Metatranscriptome-defined guilds provide a framework to model rhizosphere succession and its consequences for soil carbon cycling.
31953507	8	49	theme	Metatranscriptome-defined	1578:1602	arg1	guilds					1604:1609	Metatranscriptome-defined guilds	1578:1609	Metatranscriptome-defined guilds	1578:1609	Metatranscriptome-defined guilds provide a framework to model rhizosphere succession and its consequences for soil carbon cycling.
31953507	1	50	theme	microbial	113:121	arg1	transformations					130:144	microbial carbon transformations	113:144	microbial carbon transformations	113:144	The rhizosphere is a hotspot for microbial carbon transformations, and is the entry point for root polysaccharides and polymeric carbohydrates that are important precursors to soil organic matter (SOM).
31953507	6	51	theme	decomposition	1015:1027	arg1	genes					1029:1033	upregulated decomposition genes	1003:1033	upregulated decomposition genes	1003:1033	Using hierarchical clustering of upregulated decomposition genes, we identified four distinct microbial guilds populated by taxa whose functional succession patterns suggest specialization for substrates provided by fresh growing roots, decaying root detritus, the combination of live and decaying root biomass, or aging root material.
31953507	8	52	theme	model	1634:1638	arg1	succession					1652:1661	model rhizosphere succession	1634:1661	model rhizosphere succession	1634:1661	Metatranscriptome-defined guilds provide a framework to model rhizosphere succession and its consequences for soil carbon cycling.
31953507	7	53	theme	guilds	1530:1535	arg1	coexistence					1503:1513	coexistence	1503:1513	coexistence of rhizosphere guilds	1503:1535	Carbohydrate depolymerization genes were consistently upregulated in the rhizosphere, and both taxonomic and functional diversity were highest in the combined rhizosphere-detritusphere, suggesting coexistence of rhizosphere guilds is facilitated by niche differentiation.
31953507	1	54	theme	carbon	123:128	arg1	transformations					130:144	microbial carbon transformations	113:144	microbial carbon transformations	113:144	The rhizosphere is a hotspot for microbial carbon transformations, and is the entry point for root polysaccharides and polymeric carbohydrates that are important precursors to soil organic matter (SOM).
31953507	6	55	theme	root	1268:1271	arg1	biomass					1273:1279	live and decaying root biomass	1250:1279	live and decaying root biomass	1250:1279	Using hierarchical clustering of upregulated decomposition genes, we identified four distinct microbial guilds populated by taxa whose functional succession patterns suggest specialization for substrates provided by fresh growing roots, decaying root detritus, the combination of live and decaying root biomass, or aging root material.
31953507	5	56	theme	faster	924:929	arg1	scale					936:940	a faster time scale	922:940	a faster time scale than compositional changes	922:967	While soil habitat significantly affected both community composition and overall gene expression, the succession of microbial functions occurred at a faster time scale than compositional changes.
31953507	3	57	theme	microbial	495:503	arg1	functions					505:513	microbial functions	495:513	microbial functions in rhizosphere, detritusphere, and combined rhizosphere-detritusphere habitats	495:592	Using Avena fatua, a common annual grass, we analyzed time-resolved metatranscriptomes to compare microbial functions in rhizosphere, detritusphere, and combined rhizosphere-detritusphere habitats.
31953507	6	58	theme	succession	1116:1125	arg1	patterns					1127:1134	patterns	1127:1134	patterns	1127:1134	Using hierarchical clustering of upregulated decomposition genes, we identified four distinct microbial guilds populated by taxa whose functional succession patterns suggest specialization for substrates provided by fresh growing roots, decaying root detritus, the combination of live and decaying root biomass, or aging root material.
31953507	6	59	theme	root	1291:1294	arg1	material					1296:1303	aging root material	1285:1303	aging root material	1285:1303	Using hierarchical clustering of upregulated decomposition genes, we identified four distinct microbial guilds populated by taxa whose functional succession patterns suggest specialization for substrates provided by fresh growing roots, decaying root detritus, the combination of live and decaying root biomass, or aging root material.
31953507	7	60	theme	functional	1415:1424	arg1	diversity					1426:1434	functional diversity	1415:1434	functional diversity	1415:1434	Carbohydrate depolymerization genes were consistently upregulated in the rhizosphere, and both taxonomic and functional diversity were highest in the combined rhizosphere-detritusphere, suggesting coexistence of rhizosphere guilds is facilitated by niche differentiation.
31953507	5	61	theme	compositional	947:959	arg1	changes					961:967	compositional changes	947:967	compositional changes	947:967	While soil habitat significantly affected both community composition and overall gene expression, the succession of microbial functions occurred at a faster time scale than compositional changes.
31953507	5	62	theme	time	931:934	arg1	scale					936:940	a faster time scale	922:940	a faster time scale than compositional changes	922:967	While soil habitat significantly affected both community composition and overall gene expression, the succession of microbial functions occurred at a faster time scale than compositional changes.
31953507	4	63	theme	soil	668:671	arg1	genomes					681:687	soil isolate genomes	668:687	soil isolate genomes	668:687	Transcripts were binned using a unique reference database generated from soil isolate genomes, single-cell amplified genomes, metagenomes, and stable isotope probing metagenomes.
31953507	4	64	theme	reference	634:642	arg1	database					644:651	a unique reference database	625:651	a unique reference database generated from soil isolate genomes, single-cell amplified genomes, metagenomes, and stable isotope probing metagenomes	625:771	Transcripts were binned using a unique reference database generated from soil isolate genomes, single-cell amplified genomes, metagenomes, and stable isotope probing metagenomes.
31953507	6	65	theme	upregulated	1003:1013	arg1	genes					1029:1033	upregulated decomposition genes	1003:1033	upregulated decomposition genes	1003:1033	Using hierarchical clustering of upregulated decomposition genes, we identified four distinct microbial guilds populated by taxa whose functional succession patterns suggest specialization for substrates provided by fresh growing roots, decaying root detritus, the combination of live and decaying root biomass, or aging root material.
31953507	6	66	theme	aging	1285:1289	arg1	material					1296:1303	aging root material	1285:1303	aging root material	1285:1303	Using hierarchical clustering of upregulated decomposition genes, we identified four distinct microbial guilds populated by taxa whose functional succession patterns suggest specialization for substrates provided by fresh growing roots, decaying root detritus, the combination of live and decaying root biomass, or aging root material.
32829835	0	0	theme	nanomedicine	106:117	arg1	applications					119:130	nanobiotechnology and nanomedicine applications	84:130	nanobiotechnology and nanomedicine applications	84:130	Functionalization of developed bacterial cellulose with magnetite nanoparticles for nanobiotechnology and nanomedicine applications.
32829835	1	1	theme	magnetic	180:187	arg1	materials					189:197	novel magnetic materials	174:197	novel magnetic materials	174:197	This paper deals with the preparation of novel magnetic materials made from tetraaza macrocyclic Schiff base bacterial cellulose ligands with magnetite nanoparticles (Fe3O4NPs) through a multi-step procedure for antimicrobial and cytotoxic activities and chemotherapy in cancer treatment.
32829835	3	2	theme	co-precipitation	683:698	arg1	method					700:705	a co-precipitation method	681:705	a co-precipitation method	681:705	Then, the magnetite nanoparticles (Fe3O4NPs) was produced inside the complex [Fe(DABC-EDA-Bzl)Cl2] through a co-precipitation method.
32829835	1	3	from	activities	373:382	arg1	treatment					411:419	cancer treatment	404:419	cancer treatment	404:419	This paper deals with the preparation of novel magnetic materials made from tetraaza macrocyclic Schiff base bacterial cellulose ligands with magnetite nanoparticles (Fe3O4NPs) through a multi-step procedure for antimicrobial and cytotoxic activities and chemotherapy in cancer treatment.
32829835	1	4	theme	materials	189:197	arg1	preparation					159:169	the preparation	155:169	the preparation of novel magnetic materials made from tetraaza macrocyclic Schiff base bacterial cellulose ligands with magnetite nanoparticles (Fe3O4NPs) through a multi-step procedure for antimicrobial and cytotoxic activities and chemotherapy in cancer treatment	155:419	This paper deals with the preparation of novel magnetic materials made from tetraaza macrocyclic Schiff base bacterial cellulose ligands with magnetite nanoparticles (Fe3O4NPs) through a multi-step procedure for antimicrobial and cytotoxic activities and chemotherapy in cancer treatment.
32829835	1	5	theme	cancer	404:409	arg1	treatment					411:419	cancer treatment	404:419	cancer treatment	404:419	This paper deals with the preparation of novel magnetic materials made from tetraaza macrocyclic Schiff base bacterial cellulose ligands with magnetite nanoparticles (Fe3O4NPs) through a multi-step procedure for antimicrobial and cytotoxic activities and chemotherapy in cancer treatment.
32829835	1	6	from	antimicrobial	345:357	arg1	treatment					411:419	cancer treatment	404:419	cancer treatment	404:419	This paper deals with the preparation of novel magnetic materials made from tetraaza macrocyclic Schiff base bacterial cellulose ligands with magnetite nanoparticles (Fe3O4NPs) through a multi-step procedure for antimicrobial and cytotoxic activities and chemotherapy in cancer treatment.
32829835	5	7	theme	cytotoxic	970:978	arg1	activity					980:987	any cytotoxic activity	966:987	any cytotoxic activity towards peripheral blood mononucleocyte (PBMC) cells	966:1040	In particular, the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material have not any cytotoxic activity towards peripheral blood mononucleocyte (PBMC) cells.
32829835	5	8	theme	[Fe3O4NP-INS-	919:931	arg1	material					948:955	the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material	906:955	the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material	906:955	In particular, the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material have not any cytotoxic activity towards peripheral blood mononucleocyte (PBMC) cells.
32829835	6	9	from	delivery	1303:1310	arg1	nanomedicine					1315:1326	nanomedicine	1315:1326	nanomedicine	1315:1326	Anti-tumor studies demonstrated that the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material effectively inhibits the growth of the CT26 tumor model in BALB/c mice compared with other resulting materials throughout the experimental period and can be effective drug delivery in nanomedicine.
32829835	6	10	theme	drug	1298:1301	arg1	delivery					1303:1310	effective drug delivery	1288:1310	effective drug delivery	1288:1310	Anti-tumor studies demonstrated that the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material effectively inhibits the growth of the CT26 tumor model in BALB/c mice compared with other resulting materials throughout the experimental period and can be effective drug delivery in nanomedicine.
32829835	5	11	theme	peripheral	997:1006	arg1	cells					1036:1040	peripheral blood mononucleocyte (PBMC) cells	997:1040	peripheral blood mononucleocyte (PBMC) cells	997:1040	In particular, the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material have not any cytotoxic activity towards peripheral blood mononucleocyte (PBMC) cells.
32829835	2	12	theme	2,3-dialdehyde	433:446	arg1	DABC					469:472	DABC	469:472	DABC	469:472	First, the 2,3-dialdehyde bacterial cellulose (DABC) was chemically modified by ethylenediamine (EDA) and benzil (Bzl) in the presence of ferrous ions.
32829835	2	12	theme	2,3-dialdehyde	433:446	arg1	cellulose					458:466	the 2,3-dialdehyde bacterial cellulose	429:466	the 2,3-dialdehyde bacterial cellulose (DABC)	429:473	First, the 2,3-dialdehyde bacterial cellulose (DABC) was chemically modified by ethylenediamine (EDA) and benzil (Bzl) in the presence of ferrous ions.
32829835	6	13	theme	effective	1288:1296	arg1	delivery					1303:1310	effective drug delivery	1288:1310	effective drug delivery	1288:1310	Anti-tumor studies demonstrated that the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material effectively inhibits the growth of the CT26 tumor model in BALB/c mice compared with other resulting materials throughout the experimental period and can be effective drug delivery in nanomedicine.
32829835	5	14	theme	blood	1008:1012	arg1	cells					1036:1040	peripheral blood mononucleocyte (PBMC) cells	997:1040	peripheral blood mononucleocyte (PBMC) cells	997:1040	In particular, the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material have not any cytotoxic activity towards peripheral blood mononucleocyte (PBMC) cells.
32829835	2	15	theme	bacterial	448:456	arg1	DABC					469:472	DABC	469:472	DABC	469:472	First, the 2,3-dialdehyde bacterial cellulose (DABC) was chemically modified by ethylenediamine (EDA) and benzil (Bzl) in the presence of ferrous ions.
32829835	2	15	theme	bacterial	448:456	arg1	cellulose					458:466	the 2,3-dialdehyde bacterial cellulose	429:466	the 2,3-dialdehyde bacterial cellulose (DABC)	429:473	First, the 2,3-dialdehyde bacterial cellulose (DABC) was chemically modified by ethylenediamine (EDA) and benzil (Bzl) in the presence of ferrous ions.
32829835	5	16	theme	PBMC	1030:1033	arg1	cells					1036:1040	peripheral blood mononucleocyte (PBMC) cells	997:1040	peripheral blood mononucleocyte (PBMC) cells	997:1040	In particular, the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material have not any cytotoxic activity towards peripheral blood mononucleocyte (PBMC) cells.
32829835	3	17	theme	magnetite	584:592	arg1	Fe3O4NPs					609:616	Fe3O4NPs	609:616	Fe3O4NPs	609:616	Then, the magnetite nanoparticles (Fe3O4NPs) was produced inside the complex [Fe(DABC-EDA-Bzl)Cl2] through a co-precipitation method.
32829835	3	17	theme	magnetite	584:592	arg1	nanoparticles					594:606	the magnetite nanoparticles	580:606	the magnetite nanoparticles (Fe3O4NPs)	580:617	Then, the magnetite nanoparticles (Fe3O4NPs) was produced inside the complex [Fe(DABC-EDA-Bzl)Cl2] through a co-precipitation method.
32829835	1	18	theme	tetraaza	209:216	arg1	base					237:240	tetraaza macrocyclic Schiff base bacterial cellulose ligands	209:268	tetraaza macrocyclic Schiff base bacterial cellulose ligands with magnetite nanoparticles (Fe3O4NPs)	209:308	This paper deals with the preparation of novel magnetic materials made from tetraaza macrocyclic Schiff base bacterial cellulose ligands with magnetite nanoparticles (Fe3O4NPs) through a multi-step procedure for antimicrobial and cytotoxic activities and chemotherapy in cancer treatment.
32829835	4	19	theme	moderate	792:799	arg1	activities					829:838	moderate antimicrobial and cytotoxic activities	792:838	moderate antimicrobial and cytotoxic activities	792:838	In nanobiotechnology, the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material was showed moderate antimicrobial and cytotoxic activities against different species and cells, respectively.
32829835	6	20	theme	resulting	1222:1230	arg1	materials					1232:1240	other resulting materials	1216:1240	other resulting materials throughout the experimental period	1216:1275	Anti-tumor studies demonstrated that the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material effectively inhibits the growth of the CT26 tumor model in BALB/c mice compared with other resulting materials throughout the experimental period and can be effective drug delivery in nanomedicine.
32829835	4	21	theme	[Fe3O4NP-INS-	743:755	arg1	material					772:779	the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material	730:779	the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material	730:779	In nanobiotechnology, the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material was showed moderate antimicrobial and cytotoxic activities against different species and cells, respectively.
32829835	1	22	theme	macrocyclic	218:228	arg1	base					237:240	tetraaza macrocyclic Schiff base bacterial cellulose ligands	209:268	tetraaza macrocyclic Schiff base bacterial cellulose ligands with magnetite nanoparticles (Fe3O4NPs)	209:308	This paper deals with the preparation of novel magnetic materials made from tetraaza macrocyclic Schiff base bacterial cellulose ligands with magnetite nanoparticles (Fe3O4NPs) through a multi-step procedure for antimicrobial and cytotoxic activities and chemotherapy in cancer treatment.
32829835	0	23	theme	bacterial	31:39	arg1	cellulose					41:49	developed bacterial cellulose	21:49	developed bacterial cellulose	21:49	Functionalization of developed bacterial cellulose with magnetite nanoparticles for nanobiotechnology and nanomedicine applications.
32829835	1	24	theme	multi-step	320:329	arg1	procedure					331:339	a multi-step procedure	318:339	a multi-step procedure for antimicrobial and cytotoxic activities and chemotherapy in cancer treatment	318:419	This paper deals with the preparation of novel magnetic materials made from tetraaza macrocyclic Schiff base bacterial cellulose ligands with magnetite nanoparticles (Fe3O4NPs) through a multi-step procedure for antimicrobial and cytotoxic activities and chemotherapy in cancer treatment.
32829835	6	25	theme	tumor	1175:1179	arg1	model					1181:1185	the CT26 tumor model	1166:1185	the CT26 tumor model	1166:1185	Anti-tumor studies demonstrated that the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material effectively inhibits the growth of the CT26 tumor model in BALB/c mice compared with other resulting materials throughout the experimental period and can be effective drug delivery in nanomedicine.
32829835	1	26	theme	Schiff	230:235	arg1	base					237:240	tetraaza macrocyclic Schiff base bacterial cellulose ligands	209:268	tetraaza macrocyclic Schiff base bacterial cellulose ligands with magnetite nanoparticles (Fe3O4NPs)	209:308	This paper deals with the preparation of novel magnetic materials made from tetraaza macrocyclic Schiff base bacterial cellulose ligands with magnetite nanoparticles (Fe3O4NPs) through a multi-step procedure for antimicrobial and cytotoxic activities and chemotherapy in cancer treatment.
32829835	0	27	theme	developed	21:29	arg1	cellulose					41:49	developed bacterial cellulose	21:49	developed bacterial cellulose	21:49	Functionalization of developed bacterial cellulose with magnetite nanoparticles for nanobiotechnology and nanomedicine applications.
32829835	3	28	theme	DABC-EDA-Bzl	655:666	arg1	Cl2					668:670	the complex [Fe(DABC-EDA-Bzl)Cl2]	639:671	the complex [Fe(DABC-EDA-Bzl)Cl2]	639:671	Then, the magnetite nanoparticles (Fe3O4NPs) was produced inside the complex [Fe(DABC-EDA-Bzl)Cl2] through a co-precipitation method.
32829835	4	29	theme	DABC-EDA-Bzl	757:768	arg1	material					772:779	the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material	730:779	the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material	730:779	In nanobiotechnology, the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material was showed moderate antimicrobial and cytotoxic activities against different species and cells, respectively.
32829835	6	30	theme	model	1181:1185	arg1	growth					1156:1161	the growth	1152:1161	the growth of the CT26 tumor model in BALB/c mice	1152:1200	Anti-tumor studies demonstrated that the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material effectively inhibits the growth of the CT26 tumor model in BALB/c mice compared with other resulting materials throughout the experimental period and can be effective drug delivery in nanomedicine.
32829835	2	31	theme	ions	568:571	arg1	presence					548:555	the presence	544:555	the presence of ferrous ions	544:571	First, the 2,3-dialdehyde bacterial cellulose (DABC) was chemically modified by ethylenediamine (EDA) and benzil (Bzl) in the presence of ferrous ions.
32829835	5	32	contain	have	957:960	arg2	activity					980:987	any cytotoxic activity	966:987	any cytotoxic activity towards peripheral blood mononucleocyte (PBMC) cells	966:1040	In particular, the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material have not any cytotoxic activity towards peripheral blood mononucleocyte (PBMC) cells.
32829835	5	32	contain	have	957:960	arg1	material					948:955	the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material	906:955	the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material	906:955	In particular, the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material have not any cytotoxic activity towards peripheral blood mononucleocyte (PBMC) cells.
32829835	1	33	theme	bacterial	242:250	arg1	ligands					262:268	bacterial cellulose ligands	242:268	tetraaza macrocyclic Schiff base bacterial cellulose ligands with magnetite nanoparticles (Fe3O4NPs)	209:308	This paper deals with the preparation of novel magnetic materials made from tetraaza macrocyclic Schiff base bacterial cellulose ligands with magnetite nanoparticles (Fe3O4NPs) through a multi-step procedure for antimicrobial and cytotoxic activities and chemotherapy in cancer treatment.
32829835	0	34	theme	cellulose	41:49	arg1	Functionalization					0:16	Functionalization	0:16	Functionalization of developed bacterial cellulose with magnetite	0:64	Functionalization of developed bacterial cellulose with magnetite nanoparticles for nanobiotechnology and nanomedicine applications.
32829835	1	35	with	base	237:240	arg1	Fe3O4NPs					300:307	Fe3O4NPs	300:307	Fe3O4NPs	300:307	This paper deals with the preparation of novel magnetic materials made from tetraaza macrocyclic Schiff base bacterial cellulose ligands with magnetite nanoparticles (Fe3O4NPs) through a multi-step procedure for antimicrobial and cytotoxic activities and chemotherapy in cancer treatment.
32829835	1	35	with	base	237:240	arg1	nanoparticles					285:297	magnetite nanoparticles	275:297	magnetite nanoparticles (Fe3O4NPs)	275:308	This paper deals with the preparation of novel magnetic materials made from tetraaza macrocyclic Schiff base bacterial cellulose ligands with magnetite nanoparticles (Fe3O4NPs) through a multi-step procedure for antimicrobial and cytotoxic activities and chemotherapy in cancer treatment.
32829835	3	36	theme	[Fe	651:653	arg1	Cl2					668:670	the complex [Fe(DABC-EDA-Bzl)Cl2]	639:671	the complex [Fe(DABC-EDA-Bzl)Cl2]	639:671	Then, the magnetite nanoparticles (Fe3O4NPs) was produced inside the complex [Fe(DABC-EDA-Bzl)Cl2] through a co-precipitation method.
32829835	2	37	theme	ferrous	560:566	arg1	ions					568:571	ferrous ions	560:571	ferrous ions	560:571	First, the 2,3-dialdehyde bacterial cellulose (DABC) was chemically modified by ethylenediamine (EDA) and benzil (Bzl) in the presence of ferrous ions.
32829835	1	38	theme	cellulose	252:260	arg1	ligands					262:268	bacterial cellulose ligands	242:268	tetraaza macrocyclic Schiff base bacterial cellulose ligands with magnetite nanoparticles (Fe3O4NPs)	209:308	This paper deals with the preparation of novel magnetic materials made from tetraaza macrocyclic Schiff base bacterial cellulose ligands with magnetite nanoparticles (Fe3O4NPs) through a multi-step procedure for antimicrobial and cytotoxic activities and chemotherapy in cancer treatment.
32829835	6	39	from	growth	1156:1161	arg1	mice					1197:1200	BALB/c mice	1190:1200	BALB/c mice	1190:1200	Anti-tumor studies demonstrated that the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material effectively inhibits the growth of the CT26 tumor model in BALB/c mice compared with other resulting materials throughout the experimental period and can be effective drug delivery in nanomedicine.
32829835	2	40	mod	modified	490:497	arg3	Bzl					536:538	Bzl	536:538	Bzl	536:538	First, the 2,3-dialdehyde bacterial cellulose (DABC) was chemically modified by ethylenediamine (EDA) and benzil (Bzl) in the presence of ferrous ions.
32829835	2	40	mod	modified	490:497	arg1	cellulose					458:466	the 2,3-dialdehyde bacterial cellulose	429:466	the 2,3-dialdehyde bacterial cellulose (DABC)	429:473	First, the 2,3-dialdehyde bacterial cellulose (DABC) was chemically modified by ethylenediamine (EDA) and benzil (Bzl) in the presence of ferrous ions.
32829835	2	40	mod	modified	490:497	arg3	EDA					519:521	EDA	519:521	EDA	519:521	First, the 2,3-dialdehyde bacterial cellulose (DABC) was chemically modified by ethylenediamine (EDA) and benzil (Bzl) in the presence of ferrous ions.
32829835	2	40	mod	modified	490:497	arg3	benzil					528:533	benzil	528:533	benzil (Bzl)	528:539	First, the 2,3-dialdehyde bacterial cellulose (DABC) was chemically modified by ethylenediamine (EDA) and benzil (Bzl) in the presence of ferrous ions.
32829835	2	40	mod	modified	490:497	arg3	ethylenediamine					502:516	ethylenediamine	502:516	ethylenediamine (EDA)	502:522	First, the 2,3-dialdehyde bacterial cellulose (DABC) was chemically modified by ethylenediamine (EDA) and benzil (Bzl) in the presence of ferrous ions.
32829835	2	40	mod	modified	490:497	arg1	DABC					469:472	DABC	469:472	DABC	469:472	First, the 2,3-dialdehyde bacterial cellulose (DABC) was chemically modified by ethylenediamine (EDA) and benzil (Bzl) in the presence of ferrous ions.
32829835	6	41	theme	experimental	1257:1268	arg1	period					1270:1275	the experimental period	1253:1275	the experimental period	1253:1275	Anti-tumor studies demonstrated that the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material effectively inhibits the growth of the CT26 tumor model in BALB/c mice compared with other resulting materials throughout the experimental period and can be effective drug delivery in nanomedicine.
32829835	5	42	theme	DABC-EDA-Bzl	933:944	arg1	material					948:955	the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material	906:955	the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material	906:955	In particular, the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material have not any cytotoxic activity towards peripheral blood mononucleocyte (PBMC) cells.
32829835	1	43	from	chemotherapy	388:399	arg1	treatment					411:419	cancer treatment	404:419	cancer treatment	404:419	This paper deals with the preparation of novel magnetic materials made from tetraaza macrocyclic Schiff base bacterial cellulose ligands with magnetite nanoparticles (Fe3O4NPs) through a multi-step procedure for antimicrobial and cytotoxic activities and chemotherapy in cancer treatment.
32829835	1	44	theme	cytotoxic	363:371	arg1	activities					373:382	cytotoxic activities	363:382	cytotoxic activities	363:382	This paper deals with the preparation of novel magnetic materials made from tetraaza macrocyclic Schiff base bacterial cellulose ligands with magnetite nanoparticles (Fe3O4NPs) through a multi-step procedure for antimicrobial and cytotoxic activities and chemotherapy in cancer treatment.
32829835	6	45	theme	[Fe3O4NP-INS-	1093:1105	arg1	material					1122:1129	the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material	1080:1129	the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material	1080:1129	Anti-tumor studies demonstrated that the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material effectively inhibits the growth of the CT26 tumor model in BALB/c mice compared with other resulting materials throughout the experimental period and can be effective drug delivery in nanomedicine.
32829835	4	46	theme	different	848:856	arg1	species					858:864	different species	848:864	different species	848:864	In nanobiotechnology, the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material was showed moderate antimicrobial and cytotoxic activities against different species and cells, respectively.
32829835	6	47	theme	other	1216:1220	arg1	materials					1232:1240	other resulting materials	1216:1240	other resulting materials throughout the experimental period	1216:1275	Anti-tumor studies demonstrated that the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material effectively inhibits the growth of the CT26 tumor model in BALB/c mice compared with other resulting materials throughout the experimental period and can be effective drug delivery in nanomedicine.
32829835	5	48	theme	mononucleocyte	1014:1027	arg1	cells					1036:1040	peripheral blood mononucleocyte (PBMC) cells	997:1040	peripheral blood mononucleocyte (PBMC) cells	997:1040	In particular, the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material have not any cytotoxic activity towards peripheral blood mononucleocyte (PBMC) cells.
32829835	4	49	theme	antimicrobial	801:813	arg1	activities					829:838	moderate antimicrobial and cytotoxic activities	792:838	moderate antimicrobial and cytotoxic activities	792:838	In nanobiotechnology, the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material was showed moderate antimicrobial and cytotoxic activities against different species and cells, respectively.
32829835	0	50	with	Functionalization	0:16	arg1	magnetite					56:64	magnetite	56:64	magnetite	56:64	Functionalization of developed bacterial cellulose with magnetite nanoparticles for nanobiotechnology and nanomedicine applications.
32829835	4	51	theme	magnetic	734:741	arg1	material					772:779	the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material	730:779	the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material	730:779	In nanobiotechnology, the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material was showed moderate antimicrobial and cytotoxic activities against different species and cells, respectively.
32829835	6	52	theme	BALB/c	1190:1195	arg1	mice					1197:1200	BALB/c mice	1190:1200	BALB/c mice	1190:1200	Anti-tumor studies demonstrated that the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material effectively inhibits the growth of the CT26 tumor model in BALB/c mice compared with other resulting materials throughout the experimental period and can be effective drug delivery in nanomedicine.
32829835	3	53	theme	complex	643:649	arg1	Cl2					668:670	the complex [Fe(DABC-EDA-Bzl)Cl2]	639:671	the complex [Fe(DABC-EDA-Bzl)Cl2]	639:671	Then, the magnetite nanoparticles (Fe3O4NPs) was produced inside the complex [Fe(DABC-EDA-Bzl)Cl2] through a co-precipitation method.
32829835	5	54	theme	magnetic	910:917	arg1	material					948:955	the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material	906:955	the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material	906:955	In particular, the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material have not any cytotoxic activity towards peripheral blood mononucleocyte (PBMC) cells.
32829835	6	55	theme	Anti-tumor	1043:1052	arg1	studies					1054:1060	Anti-tumor studies	1043:1060	Anti-tumor studies	1043:1060	Anti-tumor studies demonstrated that the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material effectively inhibits the growth of the CT26 tumor model in BALB/c mice compared with other resulting materials throughout the experimental period and can be effective drug delivery in nanomedicine.
32829835	0	56	theme	nanobiotechnology	84:100	arg1	applications					119:130	nanobiotechnology and nanomedicine applications	84:130	nanobiotechnology and nanomedicine applications	84:130	Functionalization of developed bacterial cellulose with magnetite nanoparticles for nanobiotechnology and nanomedicine applications.
32829835	4	57	theme	cytotoxic	819:827	arg1	activities					829:838	moderate antimicrobial and cytotoxic activities	792:838	moderate antimicrobial and cytotoxic activities	792:838	In nanobiotechnology, the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material was showed moderate antimicrobial and cytotoxic activities against different species and cells, respectively.
32829835	6	58	theme	magnetic	1084:1091	arg1	material					1122:1129	the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material	1080:1129	the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material	1080:1129	Anti-tumor studies demonstrated that the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material effectively inhibits the growth of the CT26 tumor model in BALB/c mice compared with other resulting materials throughout the experimental period and can be effective drug delivery in nanomedicine.
32829835	6	59	theme	DABC-EDA-Bzl	1107:1118	arg1	material					1122:1129	the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material	1080:1129	the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material	1080:1129	Anti-tumor studies demonstrated that the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material effectively inhibits the growth of the CT26 tumor model in BALB/c mice compared with other resulting materials throughout the experimental period and can be effective drug delivery in nanomedicine.
32829835	1	60	theme	magnetite	275:283	arg1	Fe3O4NPs					300:307	Fe3O4NPs	300:307	Fe3O4NPs	300:307	This paper deals with the preparation of novel magnetic materials made from tetraaza macrocyclic Schiff base bacterial cellulose ligands with magnetite nanoparticles (Fe3O4NPs) through a multi-step procedure for antimicrobial and cytotoxic activities and chemotherapy in cancer treatment.
32829835	1	60	theme	magnetite	275:283	arg1	nanoparticles					285:297	magnetite nanoparticles	275:297	magnetite nanoparticles (Fe3O4NPs)	275:308	This paper deals with the preparation of novel magnetic materials made from tetraaza macrocyclic Schiff base bacterial cellulose ligands with magnetite nanoparticles (Fe3O4NPs) through a multi-step procedure for antimicrobial and cytotoxic activities and chemotherapy in cancer treatment.
32829835	6	61	theme	CT26	1170:1173	arg1	model					1181:1185	the CT26 tumor model	1166:1185	the CT26 tumor model	1166:1185	Anti-tumor studies demonstrated that the magnetic [Fe3O4NP-INS-(DABC-EDA-Bzl)] material effectively inhibits the growth of the CT26 tumor model in BALB/c mice compared with other resulting materials throughout the experimental period and can be effective drug delivery in nanomedicine.
32829835	1	62	dep	base	237:240	arg1	ligands					262:268	bacterial cellulose ligands	242:268	tetraaza macrocyclic Schiff base bacterial cellulose ligands with magnetite nanoparticles (Fe3O4NPs)	209:308	This paper deals with the preparation of novel magnetic materials made from tetraaza macrocyclic Schiff base bacterial cellulose ligands with magnetite nanoparticles (Fe3O4NPs) through a multi-step procedure for antimicrobial and cytotoxic activities and chemotherapy in cancer treatment.
32829835	1	63	theme	novel	174:178	arg1	materials					189:197	novel magnetic materials	174:197	novel magnetic materials	174:197	This paper deals with the preparation of novel magnetic materials made from tetraaza macrocyclic Schiff base bacterial cellulose ligands with magnetite nanoparticles (Fe3O4NPs) through a multi-step procedure for antimicrobial and cytotoxic activities and chemotherapy in cancer treatment.
33445606	2	0	theme	health	474:479	arg1	status					481:486	health status	474:486	health status in fathers and offspring	474:511	Our objective was to examine whether adding a methyl donor cocktail to paternal HF/S diet (HF/S+M) improves health status in fathers and offspring.
33445606	7	1	theme	fewer	898:902	arg1	kcal/day					904:911	16% fewer kcal/day	894:911	16% fewer kcal/day	894:911	HF/S+M fathers consumed 16% fewer kcal/day, which persisted in HF/S+M female offspring and was explained in part by changes in serum glucagon-like peptide-1 (GLP-1) and peptide tyrosine tyrosine (PYY) levels.
33445606	9	2	theme	Adult	1278:1282	arg1	offspring					1289:1297	Adult male offspring	1278:1297	Adult male offspring	1278:1297	In fathers, adipose tissue DNMT3a and hepatic miR-34a expression were reduced with HF/S+M. Adult male offspring showed upregulated miR-24, -33, -122a and -143 expression while females exhibited downregulated miR-33 expression.
33445606	5	3	theme	metabolic	660:668	arg1	markers					670:676	metabolic markers	660:676	metabolic markers	660:676	Body composition, metabolic markers, gut microbiota, DNA methyltransferase (DNMT) and microRNA expression were measured in fathers and offspring.
33445606	1	4	theme	male	340:343	arg1	health					358:363	male reproductive health	340:363	male reproductive health	340:363	Increased consumption of high fat/sucrose (HF/S) diets has contributed to rising rates of obesity and its co-morbidities globally, while also negatively impacting male reproductive health.
33445606	9	5	dep	HF/S+M.	1270:1276	arg1	showed					1299:1304	showed	1299:1304	showed upregulated miR-24, -33, -122a and -143 expression while females exhibited downregulated miR-33 expression	1299:1411	In fathers, adipose tissue DNMT3a and hepatic miR-34a expression were reduced with HF/S+M. Adult male offspring showed upregulated miR-24, -33, -122a and -143 expression while females exhibited downregulated miR-33 expression.
33445606	11	6	theme	gut	1600:1602	arg1	signatures					1614:1623	gut microbial signatures	1600:1623	gut microbial signatures	1600:1623	Supplementing a paternal HF/S diet with methyl-donors improved fertility, physiological outcomes, epigenetic and gut microbial signatures intergenerationally.
33445606	1	7	theme	rising	251:256	arg1	rates					258:262	rising rates	251:262	rising rates of obesity and its co-morbidities globally	251:305	Increased consumption of high fat/sucrose (HF/S) diets has contributed to rising rates of obesity and its co-morbidities globally, while also negatively impacting male reproductive health.
33445606	0	8	theme	Microbial	94:102	arg1	Signatures					104:113	Gut Microbial Signatures	90:113	Gut Microbial Signatures	90:113	Paternal Methyl Donor Supplementation in Rats Improves Fertility, Physiological Outcomes, Gut Microbial Signatures and Epigenetic Markers Altered by High Fat/High Sucrose Diet.
33445606	1	9	theme	reproductive	345:356	arg1	health					358:363	male reproductive health	340:363	male reproductive health	340:363	Increased consumption of high fat/sucrose (HF/S) diets has contributed to rising rates of obesity and its co-morbidities globally, while also negatively impacting male reproductive health.
33445606	7	10	theme	peptide-1	1017:1025	arg1	levels					1071:1076	serum glucagon-like peptide-1 (GLP-1) and peptide tyrosine tyrosine (PYY) levels	997:1076	serum glucagon-like peptide-1 (GLP-1) and peptide tyrosine tyrosine (PYY) levels	997:1076	HF/S+M fathers consumed 16% fewer kcal/day, which persisted in HF/S+M female offspring and was explained in part by changes in serum glucagon-like peptide-1 (GLP-1) and peptide tyrosine tyrosine (PYY) levels.
33445606	10	11	theme	microbial	1465:1473	arg1	signatures					1475:1484	gut microbial signatures	1461:1484	gut microbial signatures	1461:1484	Fathers and offspring presented differences in gut microbial signatures.
33445606	11	12	theme	HF/S	1512:1515	arg1	diet					1517:1520	a paternal HF/S diet	1501:1520	a paternal HF/S diet	1501:1520	Supplementing a paternal HF/S diet with methyl-donors improved fertility, physiological outcomes, epigenetic and gut microbial signatures intergenerationally.
33445606	0	13	theme	Gut	90:92	arg1	Signatures					104:113	Gut Microbial Signatures	90:113	Gut Microbial Signatures	90:113	Paternal Methyl Donor Supplementation in Rats Improves Fertility, Physiological Outcomes, Gut Microbial Signatures and Epigenetic Markers Altered by High Fat/High Sucrose Diet.
33445606	7	14	theme	HF/S+M	870:875	arg1	fathers					877:883	HF/S+M fathers	870:883	HF/S+M fathers	870:883	HF/S+M fathers consumed 16% fewer kcal/day, which persisted in HF/S+M female offspring and was explained in part by changes in serum glucagon-like peptide-1 (GLP-1) and peptide tyrosine tyrosine (PYY) levels.
33445606	10	15	from	differences	1446:1456	arg1	signatures					1475:1484	gut microbial signatures	1461:1484	gut microbial signatures	1461:1484	Fathers and offspring presented differences in gut microbial signatures.
33445606	6	16	theme	fat	835:837	arg1	mass					839:842	fat mass	835:842	fat mass	835:842	Compared to HF/S, paternal HF/S+M diet reduced fat mass in offspring (p < 0.005).
33445606	7	17	theme	serum	997:1001	arg1	GLP-1					1028:1032	GLP-1	1028:1032	GLP-1	1028:1032	HF/S+M fathers consumed 16% fewer kcal/day, which persisted in HF/S+M female offspring and was explained in part by changes in serum glucagon-like peptide-1 (GLP-1) and peptide tyrosine tyrosine (PYY) levels.
33445606	7	17	theme	serum	997:1001	arg1	peptide-1					1017:1025	serum glucagon-like peptide-1	997:1025	serum glucagon-like peptide-1 (GLP-1)	997:1033	HF/S+M fathers consumed 16% fewer kcal/day, which persisted in HF/S+M female offspring and was explained in part by changes in serum glucagon-like peptide-1 (GLP-1) and peptide tyrosine tyrosine (PYY) levels.
33445606	2	18	theme	methyl	412:417	arg1	cocktail					425:432	a methyl donor cocktail	410:432	a methyl donor cocktail	410:432	Our objective was to examine whether adding a methyl donor cocktail to paternal HF/S diet (HF/S+M) improves health status in fathers and offspring.
33445606	9	19	dep	tissue	1207:1212	arg1	DNMT3a					1214:1219	DNMT3a	1214:1219	DNMT3a	1214:1219	In fathers, adipose tissue DNMT3a and hepatic miR-34a expression were reduced with HF/S+M. Adult male offspring showed upregulated miR-24, -33, -122a and -143 expression while females exhibited downregulated miR-33 expression.
33445606	8	20	theme	33	1118:1119	arg1	%					1120:1120	%	1120:1120	%	1120:1120	Compared to HF/S, HF/S+M fathers had a 33% improvement in days until conception and 300% fewer stillbirths.
33445606	5	21	theme	DNA	695:697	arg1	DNMT					718:721	DNMT	718:721	DNMT	718:721	Body composition, metabolic markers, gut microbiota, DNA methyltransferase (DNMT) and microRNA expression were measured in fathers and offspring.
33445606	5	21	theme	DNA	695:697	arg1	methyltransferase					699:715	DNA methyltransferase	695:715	DNA methyltransferase (DNMT)	695:722	Body composition, metabolic markers, gut microbiota, DNA methyltransferase (DNMT) and microRNA expression were measured in fathers and offspring.
33445606	11	22	theme	physiological	1561:1573	arg1	outcomes					1575:1582	physiological outcomes	1561:1582	physiological outcomes	1561:1582	Supplementing a paternal HF/S diet with methyl-donors improved fertility, physiological outcomes, epigenetic and gut microbial signatures intergenerationally.
33445606	7	23	theme	glucagon-like	1003:1015	arg1	GLP-1					1028:1032	GLP-1	1028:1032	GLP-1	1028:1032	HF/S+M fathers consumed 16% fewer kcal/day, which persisted in HF/S+M female offspring and was explained in part by changes in serum glucagon-like peptide-1 (GLP-1) and peptide tyrosine tyrosine (PYY) levels.
33445606	7	23	theme	glucagon-like	1003:1015	arg1	peptide-1					1017:1025	serum glucagon-like peptide-1	997:1025	serum glucagon-like peptide-1 (GLP-1)	997:1033	HF/S+M fathers consumed 16% fewer kcal/day, which persisted in HF/S+M female offspring and was explained in part by changes in serum glucagon-like peptide-1 (GLP-1) and peptide tyrosine tyrosine (PYY) levels.
33445606	2	24	theme	paternal	437:444	arg1	HF/S+M					457:462	HF/S+M	457:462	HF/S+M	457:462	Our objective was to examine whether adding a methyl donor cocktail to paternal HF/S diet (HF/S+M) improves health status in fathers and offspring.
33445606	2	24	theme	paternal	437:444	arg1	diet					451:454	paternal HF/S diet	437:454	paternal HF/S diet (HF/S+M)	437:463	Our objective was to examine whether adding a methyl donor cocktail to paternal HF/S diet (HF/S+M) improves health status in fathers and offspring.
33445606	1	25	theme	Increased	177:185	arg1	consumption					187:197	Increased consumption	177:197	Increased consumption of high fat/sucrose (HF/S) diets	177:230	Increased consumption of high fat/sucrose (HF/S) diets has contributed to rising rates of obesity and its co-morbidities globally, while also negatively impacting male reproductive health.
33445606	0	26	theme	Methyl	9:14	arg1	Supplementation					22:36	Paternal Methyl Donor Supplementation	0:36	Paternal Methyl Donor Supplementation in Rats	0:44	Paternal Methyl Donor Supplementation in Rats Improves Fertility, Physiological Outcomes, Gut Microbial Signatures and Epigenetic Markers Altered by High Fat/High Sucrose Diet.
33445606	10	27	theme	gut	1461:1463	arg1	signatures					1475:1484	gut microbial signatures	1461:1484	gut microbial signatures	1461:1484	Fathers and offspring presented differences in gut microbial signatures.
33445606	7	28	from	changes	986:992	arg1	levels					1071:1076	serum glucagon-like peptide-1 (GLP-1) and peptide tyrosine tyrosine (PYY) levels	997:1076	serum glucagon-like peptide-1 (GLP-1) and peptide tyrosine tyrosine (PYY) levels	997:1076	HF/S+M fathers consumed 16% fewer kcal/day, which persisted in HF/S+M female offspring and was explained in part by changes in serum glucagon-like peptide-1 (GLP-1) and peptide tyrosine tyrosine (PYY) levels.
33445606	3	29	theme	male	538:541	arg1	rats					558:561	male Sprague Dawley rats	538:561	male Sprague Dawley rats	538:561	From 3-12 weeks of age, male Sprague Dawley rats consumed a HF/S or HF/S+M diet.
33445606	11	30	theme	microbial	1604:1612	arg1	signatures					1614:1623	gut microbial signatures	1600:1623	gut microbial signatures	1600:1623	Supplementing a paternal HF/S diet with methyl-donors improved fertility, physiological outcomes, epigenetic and gut microbial signatures intergenerationally.
33445606	0	31	theme	Paternal	0:7	arg1	Supplementation					22:36	Paternal Methyl Donor Supplementation	0:36	Paternal Methyl Donor Supplementation in Rats	0:44	Paternal Methyl Donor Supplementation in Rats Improves Fertility, Physiological Outcomes, Gut Microbial Signatures and Epigenetic Markers Altered by High Fat/High Sucrose Diet.
33445606	1	32	theme	obesity	267:273	arg1	rates					258:262	rising rates	251:262	rising rates of obesity and its co-morbidities globally	251:305	Increased consumption of high fat/sucrose (HF/S) diets has contributed to rising rates of obesity and its co-morbidities globally, while also negatively impacting male reproductive health.
33445606	8	33	theme	%	1120:1120	arg1	improvement					1122:1132	a 33% improvement	1116:1132	a 33% improvement in days	1116:1140	Compared to HF/S, HF/S+M fathers had a 33% improvement in days until conception and 300% fewer stillbirths.
33445606	9	34	theme	adipose	1199:1205	arg1	tissue					1207:1212	adipose tissue	1199:1212	adipose tissue	1199:1212	In fathers, adipose tissue DNMT3a and hepatic miR-34a expression were reduced with HF/S+M. Adult male offspring showed upregulated miR-24, -33, -122a and -143 expression while females exhibited downregulated miR-33 expression.
33445606	3	35	theme	age	533:535	arg1	weeks					524:528	3-12 weeks	519:528	3-12 weeks of age	519:535	From 3-12 weeks of age, male Sprague Dawley rats consumed a HF/S or HF/S+M diet.
33445606	1	36	theme	high	202:205	arg1	diets					226:230	high fat/sucrose (HF/S) diets	202:230	high fat/sucrose (HF/S) diets	202:230	Increased consumption of high fat/sucrose (HF/S) diets has contributed to rising rates of obesity and its co-morbidities globally, while also negatively impacting male reproductive health.
33445606	0	37	theme	Donor	16:20	arg1	Supplementation					22:36	Paternal Methyl Donor Supplementation	0:36	Paternal Methyl Donor Supplementation in Rats	0:44	Paternal Methyl Donor Supplementation in Rats Improves Fertility, Physiological Outcomes, Gut Microbial Signatures and Epigenetic Markers Altered by High Fat/High Sucrose Diet.
33445606	0	38	theme	Epigenetic	119:128	arg1	Markers					130:136	Epigenetic Markers	119:136	Epigenetic Markers	119:136	Paternal Methyl Donor Supplementation in Rats Improves Fertility, Physiological Outcomes, Gut Microbial Signatures and Epigenetic Markers Altered by High Fat/High Sucrose Diet.
33445606	6	39	theme	HF/S+M	815:820	arg1	diet					822:825	paternal HF/S+M diet	806:825	paternal HF/S+M diet	806:825	Compared to HF/S, paternal HF/S+M diet reduced fat mass in offspring (p < 0.005).
33445606	1	40	theme	fat/sucrose	207:217	arg1	diets					226:230	high fat/sucrose (HF/S) diets	202:230	high fat/sucrose (HF/S) diets	202:230	Increased consumption of high fat/sucrose (HF/S) diets has contributed to rising rates of obesity and its co-morbidities globally, while also negatively impacting male reproductive health.
33445606	7	41	theme	tyrosine	1047:1054	arg1	PYY					1066:1068	peptide tyrosine tyrosine (PYY)	1039:1069	peptide tyrosine tyrosine (PYY)	1039:1069	HF/S+M fathers consumed 16% fewer kcal/day, which persisted in HF/S+M female offspring and was explained in part by changes in serum glucagon-like peptide-1 (GLP-1) and peptide tyrosine tyrosine (PYY) levels.
33445606	1	42	theme	co-morbidities	283:296	arg1	rates					258:262	rising rates	251:262	rising rates of obesity and its co-morbidities globally	251:305	Increased consumption of high fat/sucrose (HF/S) diets has contributed to rising rates of obesity and its co-morbidities globally, while also negatively impacting male reproductive health.
33445606	6	43	theme	paternal	806:813	arg1	diet					822:825	paternal HF/S+M diet	806:825	paternal HF/S+M diet	806:825	Compared to HF/S, paternal HF/S+M diet reduced fat mass in offspring (p < 0.005).
33445606	5	44	theme	microRNA	728:735	arg1	expression					737:746	microRNA expression	728:746	microRNA expression	728:746	Body composition, metabolic markers, gut microbiota, DNA methyltransferase (DNMT) and microRNA expression were measured in fathers and offspring.
33445606	7	45	theme	tyrosine	1056:1063	arg1	PYY					1066:1068	peptide tyrosine tyrosine (PYY)	1039:1069	peptide tyrosine tyrosine (PYY)	1039:1069	HF/S+M fathers consumed 16% fewer kcal/day, which persisted in HF/S+M female offspring and was explained in part by changes in serum glucagon-like peptide-1 (GLP-1) and peptide tyrosine tyrosine (PYY) levels.
33445606	8	46	contain	had	1112:1114	arg2	improvement					1122:1132	a 33% improvement	1116:1132	a 33% improvement in days	1116:1140	Compared to HF/S, HF/S+M fathers had a 33% improvement in days until conception and 300% fewer stillbirths.
33445606	8	46	contain	had	1112:1114	arg1	fathers					1104:1110	HF/S+M fathers	1097:1110	HF/S+M fathers	1097:1110	Compared to HF/S, HF/S+M fathers had a 33% improvement in days until conception and 300% fewer stillbirths.
33445606	8	47	from	improvement	1122:1132	arg1	days					1137:1140	days	1137:1140	days	1137:1140	Compared to HF/S, HF/S+M fathers had a 33% improvement in days until conception and 300% fewer stillbirths.
33445606	1	48	theme	HF/S	220:223	arg1	diets					226:230	high fat/sucrose (HF/S) diets	202:230	high fat/sucrose (HF/S) diets	202:230	Increased consumption of high fat/sucrose (HF/S) diets has contributed to rising rates of obesity and its co-morbidities globally, while also negatively impacting male reproductive health.
33445606	0	49	theme	Fertility	55:63	arg1	Outcomes					80:87	Fertility, Physiological Outcomes	55:87	Outcomes	80:87	Paternal Methyl Donor Supplementation in Rats Improves Fertility, Physiological Outcomes, Gut Microbial Signatures and Epigenetic Markers Altered by High Fat/High Sucrose Diet.
33445606	5	50	theme	gut	679:681	arg1	microbiota					683:692	gut microbiota	679:692	gut microbiota	679:692	Body composition, metabolic markers, gut microbiota, DNA methyltransferase (DNMT) and microRNA expression were measured in fathers and offspring.
33445606	0	51	theme	Fat/High	154:161	arg1	Diet					171:174	High Fat/High Sucrose Diet	149:174	High Fat/High Sucrose Diet	149:174	Paternal Methyl Donor Supplementation in Rats Improves Fertility, Physiological Outcomes, Gut Microbial Signatures and Epigenetic Markers Altered by High Fat/High Sucrose Diet.
33445606	2	52	from	status	481:486	arg1	offspring					503:511	offspring	503:511	offspring	503:511	Our objective was to examine whether adding a methyl donor cocktail to paternal HF/S diet (HF/S+M) improves health status in fathers and offspring.
33445606	2	52	from	status	481:486	arg1	fathers					491:497	fathers	491:497	fathers	491:497	Our objective was to examine whether adding a methyl donor cocktail to paternal HF/S diet (HF/S+M) improves health status in fathers and offspring.
33445606	3	53	theme	Dawley	551:556	arg1	rats					558:561	male Sprague Dawley rats	538:561	male Sprague Dawley rats	538:561	From 3-12 weeks of age, male Sprague Dawley rats consumed a HF/S or HF/S+M diet.
33445606	7	54	theme	HF/S+M	933:938	arg1	offspring					947:955	HF/S+M female offspring	933:955	HF/S+M female offspring	933:955	HF/S+M fathers consumed 16% fewer kcal/day, which persisted in HF/S+M female offspring and was explained in part by changes in serum glucagon-like peptide-1 (GLP-1) and peptide tyrosine tyrosine (PYY) levels.
33445606	7	55	theme	peptide	1039:1045	arg1	PYY					1066:1068	peptide tyrosine tyrosine (PYY)	1039:1069	peptide tyrosine tyrosine (PYY)	1039:1069	HF/S+M fathers consumed 16% fewer kcal/day, which persisted in HF/S+M female offspring and was explained in part by changes in serum glucagon-like peptide-1 (GLP-1) and peptide tyrosine tyrosine (PYY) levels.
33445606	9	56	theme	miR-34a	1233:1239	arg1	expression					1241:1250	hepatic miR-34a expression	1225:1250	hepatic miR-34a expression	1225:1250	In fathers, adipose tissue DNMT3a and hepatic miR-34a expression were reduced with HF/S+M. Adult male offspring showed upregulated miR-24, -33, -122a and -143 expression while females exhibited downregulated miR-33 expression.
33445606	0	57	theme	High	149:152	arg1	Diet					171:174	High Fat/High Sucrose Diet	149:174	High Fat/High Sucrose Diet	149:174	Paternal Methyl Donor Supplementation in Rats Improves Fertility, Physiological Outcomes, Gut Microbial Signatures and Epigenetic Markers Altered by High Fat/High Sucrose Diet.
33445606	7	58	theme	female	940:945	arg1	offspring					947:955	HF/S+M female offspring	933:955	HF/S+M female offspring	933:955	HF/S+M fathers consumed 16% fewer kcal/day, which persisted in HF/S+M female offspring and was explained in part by changes in serum glucagon-like peptide-1 (GLP-1) and peptide tyrosine tyrosine (PYY) levels.
33445606	8	59	theme	HF/S+M	1097:1102	arg1	fathers					1104:1110	HF/S+M fathers	1097:1110	HF/S+M fathers	1097:1110	Compared to HF/S, HF/S+M fathers had a 33% improvement in days until conception and 300% fewer stillbirths.
33445606	6	60	theme	<	860:860	arg1	offspring					847:855	offspring	847:855	offspring (p < 0.005)	847:867	Compared to HF/S, paternal HF/S+M diet reduced fat mass in offspring (p < 0.005).
33445606	6	60	theme	<	860:860	arg1	p					858:858	p < 0.005	858:866	p < 0.005	858:866	Compared to HF/S, paternal HF/S+M diet reduced fat mass in offspring (p < 0.005).
33445606	5	61	theme	Body	642:645	arg1	composition					647:657	Body composition	642:657	Body composition	642:657	Body composition, metabolic markers, gut microbiota, DNA methyltransferase (DNMT) and microRNA expression were measured in fathers and offspring.
33445606	9	62	dep	showed	1299:1304	arg1	upregulated					1306:1316	upregulated	1306:1316	showed upregulated miR-24, -33, -122a and -143 expression while females exhibited downregulated miR-33 expression	1299:1411	In fathers, adipose tissue DNMT3a and hepatic miR-34a expression were reduced with HF/S+M. Adult male offspring showed upregulated miR-24, -33, -122a and -143 expression while females exhibited downregulated miR-33 expression.
33445606	3	63	theme	HF/S+M	582:587	arg1	diet					589:592	a HF/S or HF/S+M diet	572:592	diet	589:592	From 3-12 weeks of age, male Sprague Dawley rats consumed a HF/S or HF/S+M diet.
33445606	9	64	theme	miR-33	1395:1400	arg1	expression					1402:1411	downregulated miR-33 expression	1381:1411	downregulated miR-33 expression	1381:1411	In fathers, adipose tissue DNMT3a and hepatic miR-34a expression were reduced with HF/S+M. Adult male offspring showed upregulated miR-24, -33, -122a and -143 expression while females exhibited downregulated miR-33 expression.
33445606	8	65	theme	fewer	1168:1172	arg1	stillbirths					1174:1184	300% fewer stillbirths	1163:1184	300% fewer stillbirths	1163:1184	Compared to HF/S, HF/S+M fathers had a 33% improvement in days until conception and 300% fewer stillbirths.
33445606	0	66	theme	Sucrose	163:169	arg1	Diet					171:174	High Fat/High Sucrose Diet	149:174	High Fat/High Sucrose Diet	149:174	Paternal Methyl Donor Supplementation in Rats Improves Fertility, Physiological Outcomes, Gut Microbial Signatures and Epigenetic Markers Altered by High Fat/High Sucrose Diet.
33445606	9	67	theme	male	1284:1287	arg1	offspring					1289:1297	Adult male offspring	1278:1297	Adult male offspring	1278:1297	In fathers, adipose tissue DNMT3a and hepatic miR-34a expression were reduced with HF/S+M. Adult male offspring showed upregulated miR-24, -33, -122a and -143 expression while females exhibited downregulated miR-33 expression.
33445606	4	68	theme	age	637:639	arg1	weeks					628:632	16 weeks	625:632	16 weeks of age	625:639	Offspring were followed until 16 weeks of age.
33445606	1	69	theme	diets	226:230	arg1	consumption					187:197	Increased consumption	177:197	Increased consumption of high fat/sucrose (HF/S) diets	177:230	Increased consumption of high fat/sucrose (HF/S) diets has contributed to rising rates of obesity and its co-morbidities globally, while also negatively impacting male reproductive health.
33445606	0	70	theme	Physiological	66:78	arg1	Outcomes					80:87	Fertility, Physiological Outcomes	55:87	Outcomes	80:87	Paternal Methyl Donor Supplementation in Rats Improves Fertility, Physiological Outcomes, Gut Microbial Signatures and Epigenetic Markers Altered by High Fat/High Sucrose Diet.
33445606	3	71	theme	HF/S	574:577	arg1	diet					589:592	a HF/S or HF/S+M diet	572:592	diet	589:592	From 3-12 weeks of age, male Sprague Dawley rats consumed a HF/S or HF/S+M diet.
33445606	2	72	theme	donor	419:423	arg1	cocktail					425:432	a methyl donor cocktail	410:432	a methyl donor cocktail	410:432	Our objective was to examine whether adding a methyl donor cocktail to paternal HF/S diet (HF/S+M) improves health status in fathers and offspring.
33445606	9	73	theme	downregulated	1381:1393	arg1	expression					1402:1411	downregulated miR-33 expression	1381:1411	downregulated miR-33 expression	1381:1411	In fathers, adipose tissue DNMT3a and hepatic miR-34a expression were reduced with HF/S+M. Adult male offspring showed upregulated miR-24, -33, -122a and -143 expression while females exhibited downregulated miR-33 expression.
33445606	0	74	from	Supplementation	22:36	arg1	Rats					41:44	Rats	41:44	Rats	41:44	Paternal Methyl Donor Supplementation in Rats Improves Fertility, Physiological Outcomes, Gut Microbial Signatures and Epigenetic Markers Altered by High Fat/High Sucrose Diet.
33445606	7	75	theme	PYY	1066:1068	arg1	levels					1071:1076	serum glucagon-like peptide-1 (GLP-1) and peptide tyrosine tyrosine (PYY) levels	997:1076	serum glucagon-like peptide-1 (GLP-1) and peptide tyrosine tyrosine (PYY) levels	997:1076	HF/S+M fathers consumed 16% fewer kcal/day, which persisted in HF/S+M female offspring and was explained in part by changes in serum glucagon-like peptide-1 (GLP-1) and peptide tyrosine tyrosine (PYY) levels.
33445606	11	76	theme	paternal	1503:1510	arg1	diet					1517:1520	a paternal HF/S diet	1501:1520	a paternal HF/S diet	1501:1520	Supplementing a paternal HF/S diet with methyl-donors improved fertility, physiological outcomes, epigenetic and gut microbial signatures intergenerationally.
33445606	2	77	theme	HF/S	446:449	arg1	HF/S+M					457:462	HF/S+M	457:462	HF/S+M	457:462	Our objective was to examine whether adding a methyl donor cocktail to paternal HF/S diet (HF/S+M) improves health status in fathers and offspring.
33445606	2	77	theme	HF/S	446:449	arg1	diet					451:454	paternal HF/S diet	437:454	paternal HF/S diet (HF/S+M)	437:463	Our objective was to examine whether adding a methyl donor cocktail to paternal HF/S diet (HF/S+M) improves health status in fathers and offspring.
33445606	9	78	theme	hepatic	1225:1231	arg1	expression					1241:1250	hepatic miR-34a expression	1225:1250	hepatic miR-34a expression	1225:1250	In fathers, adipose tissue DNMT3a and hepatic miR-34a expression were reduced with HF/S+M. Adult male offspring showed upregulated miR-24, -33, -122a and -143 expression while females exhibited downregulated miR-33 expression.
31674893	8	0	theme	peptidoglycan	1098:1110	arg1	acids					1118:1122	The cell-wall peptidoglycan amino acids	1084:1122	The cell-wall peptidoglycan amino acids	1084:1122	The cell-wall peptidoglycan amino acids were meso-2,6-diaminopimelic acid, alanine and glutamic acid; the major cell-wall sugar was galactose.
31674893	8	0	theme	peptidoglycan	1098:1110	arg1	acid					1153:1156	meso-2,6-diaminopimelic acid	1129:1156	meso-2,6-diaminopimelic acid	1129:1156	The cell-wall peptidoglycan amino acids were meso-2,6-diaminopimelic acid, alanine and glutamic acid; the major cell-wall sugar was galactose.
31674893	6	1	theme	C18 	838:841	arg1	menaquinone					920:930	the predominant menaquinone	904:930	the predominant menaquinone	904:930	Cells contained C18 : 1 ω7c as the major cellular fatty acid and ubiquinone-10 as the predominant menaquinone.
31674893	6	1	theme	C18 	838:841	arg1	ω7c					846:848	C18 : 1 ω7c	838:848	C18 : 1 ω7c	838:848	Cells contained C18 : 1 ω7c as the major cellular fatty acid and ubiquinone-10 as the predominant menaquinone.
31674893	6	1	theme	C18 	838:841	arg1	acid					878:881	the major cellular fatty acid	853:881	the major cellular fatty acid	853:881	Cells contained C18 : 1 ω7c as the major cellular fatty acid and ubiquinone-10 as the predominant menaquinone.
31674893	7	2	theme	unidentified	1064:1075	arg1	lipid					1077:1081	an unidentified lipid	1061:1081	an unidentified lipid	1061:1081	The polar lipids comprised phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phospholipid, glycolipid and an unidentified lipid.
31674893	8	3	theme	amino	1112:1116	arg1	acids					1118:1122	The cell-wall peptidoglycan amino acids	1084:1122	The cell-wall peptidoglycan amino acids	1084:1122	The cell-wall peptidoglycan amino acids were meso-2,6-diaminopimelic acid, alanine and glutamic acid; the major cell-wall sugar was galactose.
31674893	8	3	theme	amino	1112:1116	arg1	acid					1153:1156	meso-2,6-diaminopimelic acid	1129:1156	meso-2,6-diaminopimelic acid	1129:1156	The cell-wall peptidoglycan amino acids were meso-2,6-diaminopimelic acid, alanine and glutamic acid; the major cell-wall sugar was galactose.
31674893	8	4	theme	cell-wall	1088:1096	arg1	acids					1118:1122	The cell-wall peptidoglycan amino acids	1084:1122	The cell-wall peptidoglycan amino acids	1084:1122	The cell-wall peptidoglycan amino acids were meso-2,6-diaminopimelic acid, alanine and glutamic acid; the major cell-wall sugar was galactose.
31674893	8	4	theme	cell-wall	1088:1096	arg1	acid					1153:1156	meso-2,6-diaminopimelic acid	1129:1156	meso-2,6-diaminopimelic acid	1129:1156	The cell-wall peptidoglycan amino acids were meso-2,6-diaminopimelic acid, alanine and glutamic acid; the major cell-wall sugar was galactose.
31674893	3	5	theme	97.6	620:623	arg1	%					634:634	97.6 and 97.8 %	620:634	%	634:634	The results of phylogenetic analyses of 16S rRNA gene sequences indicated that strains 2251T and 3058 represent a new species within the genus Paracoccus and are most similar to 'Paracoccus gahaiensis' CUG00006T (98.9 and 99.3 %), Paracoccus nototheniae I-41R45T (98.3 and 98.7 %) and Paracoccus hibiscisoli THG-T2.31T (97.6 and 97.8 %).
31674893	3	5	theme	97.6	620:623	arg1	THG-T2.31T					608:617	Paracoccus hibiscisoli THG-T2.31T	585:617	Paracoccus hibiscisoli THG-T2.31T (97.6 and 97.8 %)	585:635	The results of phylogenetic analyses of 16S rRNA gene sequences indicated that strains 2251T and 3058 represent a new species within the genus Paracoccus and are most similar to 'Paracoccus gahaiensis' CUG00006T (98.9 and 99.3 %), Paracoccus nototheniae I-41R45T (98.3 and 98.7 %) and Paracoccus hibiscisoli THG-T2.31T (97.6 and 97.8 %).
31674893	2	6	theme	non-motile	170:179	arg1	bacilli					210:216	aerobic, Gram-stain-negative, non-motile, coccoid or short rod-shaped bacilli	140:216	aerobic, Gram-stain-negative, non-motile, coccoid or short rod-shaped bacilli	140:216	Two strains, designated 2251T and 3058, that were aerobic, Gram-stain-negative, non-motile, coccoid or short rod-shaped bacilli, have recently been isolated from Tibetan antelopes on the Qinghai-Tibet Plateau.
31674893	10	7	theme	less	1335:1338	arg1	values					1328:1333	DNA-DNA relatedness values	1308:1333	DNA-DNA relatedness values less than 50 % with all available genomes of the genus Paracoccus in the ncbi database	1308:1420	Both strains (2251T and 3058) had DNA-DNA relatedness values less than 50 % with all available genomes of the genus Paracoccus in the ncbi database.
31674893	11	8	theme	Differential	1423:1434	arg1	inferences					1446:1455	Differential genotypic inferences	1423:1455	Differential genotypic inferences	1423:1455	Differential genotypic inferences, together with phenotypic and biochemical characteristics, demonstrated that strains 2251T and 3058 should be classified as a novel species of the genus Paracoccus, for which the name Paracoccus liaowanqingii sp.
31674893	8	9	theme	major	1190:1194	arg1	sugar					1206:1210	the major cell-wall sugar	1186:1210	the major cell-wall sugar	1186:1210	The cell-wall peptidoglycan amino acids were meso-2,6-diaminopimelic acid, alanine and glutamic acid; the major cell-wall sugar was galactose.
31674893	8	9	theme	major	1190:1194	arg1	galactose					1216:1224	galactose	1216:1224	galactose	1216:1224	The cell-wall peptidoglycan amino acids were meso-2,6-diaminopimelic acid, alanine and glutamic acid; the major cell-wall sugar was galactose.
31674893	9	10	theme	2251T	1253:1257	arg1	content					1235:1241	The G+C content	1227:1241	The G+C content of strain 2251T	1227:1257	The G+C content of strain 2251T was 66.5 mol%.
31674893	9	10	theme	2251T	1253:1257	arg1	%					1271:1271	66.5 mol%	1263:1271	66.5 mol%	1263:1271	The G+C content of strain 2251T was 66.5 mol%.
31674893	10	11	theme	available	1359:1367	arg1	genomes					1369:1375	all available genomes	1355:1375	all available genomes of the genus Paracoccus in the ncbi database	1355:1420	Both strains (2251T and 3058) had DNA-DNA relatedness values less than 50 % with all available genomes of the genus Paracoccus in the ncbi database.
31674893	1	12	theme	Tibetan	49:55	arg1	antelope					57:64	Tibetan antelope	49:64	Tibetan antelope (Pantholops hodgsonii)	49:87	nov., isolated from Tibetan antelope (Pantholops hodgsonii).
31674893	1	12	theme	Tibetan	49:55	arg1	hodgsonii					78:86	Pantholops hodgsonii	67:86	Pantholops hodgsonii	67:86	nov., isolated from Tibetan antelope (Pantholops hodgsonii).
31674893	11	13	theme	genotypic	1436:1444	arg1	inferences					1446:1455	Differential genotypic inferences	1423:1455	Differential genotypic inferences	1423:1455	Differential genotypic inferences, together with phenotypic and biochemical characteristics, demonstrated that strains 2251T and 3058 should be classified as a novel species of the genus Paracoccus, for which the name Paracoccus liaowanqingii sp.
31674893	13	14	theme	=CGMCC	1715:1720	arg1	106269T					1735:1741	=CGMCC 1.16490T=DSM 106269T	1715:1741	=CGMCC 1.16490T=DSM 106269T	1715:1741	The type strain is 2251T (=CGMCC 1.16490T=DSM 106269T).
31674893	13	14	theme	=CGMCC	1715:1720	arg1	2251T					1708:1712	2251T	1708:1712	2251T (=CGMCC 1.16490T=DSM 106269T)	1708:1742	The type strain is 2251T (=CGMCC 1.16490T=DSM 106269T).
31674893	3	15	theme	16S	340:342	arg1	sequences					354:362	16S rRNA gene sequences	340:362	16S rRNA gene sequences	340:362	The results of phylogenetic analyses of 16S rRNA gene sequences indicated that strains 2251T and 3058 represent a new species within the genus Paracoccus and are most similar to 'Paracoccus gahaiensis' CUG00006T (98.9 and 99.3 %), Paracoccus nototheniae I-41R45T (98.3 and 98.7 %) and Paracoccus hibiscisoli THG-T2.31T (97.6 and 97.8 %).
31674893	11	16	theme	genus	1604:1608	arg1	Paracoccus					1610:1619	the genus Paracoccus	1600:1619	the genus Paracoccus	1600:1619	Differential genotypic inferences, together with phenotypic and biochemical characteristics, demonstrated that strains 2251T and 3058 should be classified as a novel species of the genus Paracoccus, for which the name Paracoccus liaowanqingii sp.
31674893	10	17	theme	relatedness	1316:1326	arg1	values					1328:1333	DNA-DNA relatedness values	1308:1333	DNA-DNA relatedness values less than 50 % with all available genomes of the genus Paracoccus in the ncbi database	1308:1420	Both strains (2251T and 3058) had DNA-DNA relatedness values less than 50 % with all available genomes of the genus Paracoccus in the ncbi database.
31674893	2	18	theme	Tibetan	252:258	arg1	antelopes					260:268	Tibetan antelopes	252:268	Tibetan antelopes	252:268	Two strains, designated 2251T and 3058, that were aerobic, Gram-stain-negative, non-motile, coccoid or short rod-shaped bacilli, have recently been isolated from Tibetan antelopes on the Qinghai-Tibet Plateau.
31674893	3	19	theme	phylogenetic	315:326	arg1	analyses					328:335	phylogenetic analyses	315:335	phylogenetic analyses of 16S rRNA gene sequences	315:362	The results of phylogenetic analyses of 16S rRNA gene sequences indicated that strains 2251T and 3058 represent a new species within the genus Paracoccus and are most similar to 'Paracoccus gahaiensis' CUG00006T (98.9 and 99.3 %), Paracoccus nototheniae I-41R45T (98.3 and 98.7 %) and Paracoccus hibiscisoli THG-T2.31T (97.6 and 97.8 %).
31674893	9	20	theme	66.5 mol	1263:1270	arg1	content					1235:1241	The G+C content	1227:1241	The G+C content of strain 2251T	1227:1257	The G+C content of strain 2251T was 66.5 mol%.
31674893	9	20	theme	66.5 mol	1263:1270	arg1	%					1271:1271	66.5 mol%	1263:1271	66.5 mol%	1263:1271	The G+C content of strain 2251T was 66.5 mol%.
31674893	8	21	theme	meso-2,6-diaminopimelic	1129:1151	arg1	acids					1118:1122	The cell-wall peptidoglycan amino acids	1084:1122	The cell-wall peptidoglycan amino acids	1084:1122	The cell-wall peptidoglycan amino acids were meso-2,6-diaminopimelic acid, alanine and glutamic acid; the major cell-wall sugar was galactose.
31674893	8	21	theme	meso-2,6-diaminopimelic	1129:1151	arg1	acid					1153:1156	meso-2,6-diaminopimelic acid	1129:1156	meso-2,6-diaminopimelic acid	1129:1156	The cell-wall peptidoglycan amino acids were meso-2,6-diaminopimelic acid, alanine and glutamic acid; the major cell-wall sugar was galactose.
31674893	10	22	theme	DNA-DNA	1308:1314	arg1	values					1328:1333	DNA-DNA relatedness values	1308:1333	DNA-DNA relatedness values less than 50 % with all available genomes of the genus Paracoccus in the ncbi database	1308:1420	Both strains (2251T and 3058) had DNA-DNA relatedness values less than 50 % with all available genomes of the genus Paracoccus in the ncbi database.
31674893	3	23	theme	gahaiensis	490:499	arg1	CUG00006T					502:510	'Paracoccus gahaiensis' CUG00006T	478:510	'Paracoccus gahaiensis' CUG00006T (98.9 and 99.3 %)	478:528	The results of phylogenetic analyses of 16S rRNA gene sequences indicated that strains 2251T and 3058 represent a new species within the genus Paracoccus and are most similar to 'Paracoccus gahaiensis' CUG00006T (98.9 and 99.3 %), Paracoccus nototheniae I-41R45T (98.3 and 98.7 %) and Paracoccus hibiscisoli THG-T2.31T (97.6 and 97.8 %).
31674893	3	23	theme	gahaiensis	490:499	arg1	%					527:527	98.9 and 99.3 %	513:527	98.9 and 99.3 %	513:527	The results of phylogenetic analyses of 16S rRNA gene sequences indicated that strains 2251T and 3058 represent a new species within the genus Paracoccus and are most similar to 'Paracoccus gahaiensis' CUG00006T (98.9 and 99.3 %), Paracoccus nototheniae I-41R45T (98.3 and 98.7 %) and Paracoccus hibiscisoli THG-T2.31T (97.6 and 97.8 %).
31674893	8	24	theme	cell-wall	1196:1204	arg1	sugar					1206:1210	the major cell-wall sugar	1186:1210	the major cell-wall sugar	1186:1210	The cell-wall peptidoglycan amino acids were meso-2,6-diaminopimelic acid, alanine and glutamic acid; the major cell-wall sugar was galactose.
31674893	8	24	theme	cell-wall	1196:1204	arg1	galactose					1216:1224	galactose	1216:1224	galactose	1216:1224	The cell-wall peptidoglycan amino acids were meso-2,6-diaminopimelic acid, alanine and glutamic acid; the major cell-wall sugar was galactose.
31674893	3	25	theme	analyses	328:335	arg1	results					304:310	The results	300:310	The results of phylogenetic analyses of 16S rRNA gene sequences	300:362	The results of phylogenetic analyses of 16S rRNA gene sequences indicated that strains 2251T and 3058 represent a new species within the genus Paracoccus and are most similar to 'Paracoccus gahaiensis' CUG00006T (98.9 and 99.3 %), Paracoccus nototheniae I-41R45T (98.3 and 98.7 %) and Paracoccus hibiscisoli THG-T2.31T (97.6 and 97.8 %).
31674893	10	26	theme	Paracoccus	1390:1399	arg1	genomes					1369:1375	all available genomes	1355:1375	all available genomes of the genus Paracoccus in the ncbi database	1355:1420	Both strains (2251T and 3058) had DNA-DNA relatedness values less than 50 % with all available genomes of the genus Paracoccus in the ncbi database.
31674893	1	27	theme	Pantholops	67:76	arg1	antelope					57:64	Tibetan antelope	49:64	Tibetan antelope (Pantholops hodgsonii)	49:87	nov., isolated from Tibetan antelope (Pantholops hodgsonii).
31674893	1	27	theme	Pantholops	67:76	arg1	hodgsonii					78:86	Pantholops hodgsonii	67:86	Pantholops hodgsonii	67:86	nov., isolated from Tibetan antelope (Pantholops hodgsonii).
31674893	3	28	theme	new	414:416	arg1	species					418:424	a new species	412:424	a new species within the genus Paracoccus	412:452	The results of phylogenetic analyses of 16S rRNA gene sequences indicated that strains 2251T and 3058 represent a new species within the genus Paracoccus and are most similar to 'Paracoccus gahaiensis' CUG00006T (98.9 and 99.3 %), Paracoccus nototheniae I-41R45T (98.3 and 98.7 %) and Paracoccus hibiscisoli THG-T2.31T (97.6 and 97.8 %).
31674893	0	29	theme	liaowanqingii	11:23	arg1	sp					25:26	Paracoccus liaowanqingii sp	0:26	Paracoccus liaowanqingii sp.	0:27	Paracoccus liaowanqingii sp.
31674893	10	30	from	database	1413:1420	arg1	genomes					1369:1375	all available genomes	1355:1375	all available genomes of the genus Paracoccus in the ncbi database	1355:1420	Both strains (2251T and 3058) had DNA-DNA relatedness values less than 50 % with all available genomes of the genus Paracoccus in the ncbi database.
31674893	10	31	theme	genus	1384:1388	arg1	Paracoccus					1390:1399	the genus Paracoccus	1380:1399	the genus Paracoccus in the ncbi database	1380:1420	Both strains (2251T and 3058) had DNA-DNA relatedness values less than 50 % with all available genomes of the genus Paracoccus in the ncbi database.
31674893	4	32	theme	sequence-based	657:670	arg1	analysis					685:692	genomic sequence-based phylogenomic analysis	649:692	genomic sequence-based phylogenomic analysis	649:692	Results of genomic sequence-based phylogenomic analysis agreed with those from 16S rRNA gene sequence analysis.
31674893	11	33	theme	Paracoccus	1610:1619	arg1	species					1589:1595	a novel species	1581:1595	a novel species	1581:1595	Differential genotypic inferences, together with phenotypic and biochemical characteristics, demonstrated that strains 2251T and 3058 should be classified as a novel species of the genus Paracoccus, for which the name Paracoccus liaowanqingii sp.
31674893	11	33	theme	Paracoccus	1610:1619	arg1	strains					1534:1540	strains 2251T and 3058	1534:1555	strains	1534:1540	Differential genotypic inferences, together with phenotypic and biochemical characteristics, demonstrated that strains 2251T and 3058 should be classified as a novel species of the genus Paracoccus, for which the name Paracoccus liaowanqingii sp.
31674893	11	33	theme	Paracoccus	1610:1619	arg1	3058					1552:1555	3058	1552:1555	3058	1552:1555	Differential genotypic inferences, together with phenotypic and biochemical characteristics, demonstrated that strains 2251T and 3058 should be classified as a novel species of the genus Paracoccus, for which the name Paracoccus liaowanqingii sp.
31674893	0	34	theme	Paracoccus	0:9	arg1	sp					25:26	Paracoccus liaowanqingii sp	0:26	Paracoccus liaowanqingii sp.	0:27	Paracoccus liaowanqingii sp.
31674893	9	35	theme	G+C	1231:1233	arg1	content					1235:1241	The G+C content	1227:1241	The G+C content of strain 2251T	1227:1257	The G+C content of strain 2251T was 66.5 mol%.
31674893	9	35	theme	G+C	1231:1233	arg1	%					1271:1271	66.5 mol%	1263:1271	66.5 mol%	1263:1271	The G+C content of strain 2251T was 66.5 mol%.
31674893	11	36	theme	biochemical	1487:1497	arg1	characteristics					1499:1513	phenotypic and biochemical characteristics	1472:1513	phenotypic and biochemical characteristics	1472:1513	Differential genotypic inferences, together with phenotypic and biochemical characteristics, demonstrated that strains 2251T and 3058 should be classified as a novel species of the genus Paracoccus, for which the name Paracoccus liaowanqingii sp.
31674893	2	37	theme	Qinghai-Tibet	277:289	arg1	Plateau					291:297	the Qinghai-Tibet Plateau	273:297	the Qinghai-Tibet Plateau	273:297	Two strains, designated 2251T and 3058, that were aerobic, Gram-stain-negative, non-motile, coccoid or short rod-shaped bacilli, have recently been isolated from Tibetan antelopes on the Qinghai-Tibet Plateau.
31674893	10	38	dep	strains	1279:1285	arg1	3058					1298:1301	3058	1298:1301	3058	1298:1301	Both strains (2251T and 3058) had DNA-DNA relatedness values less than 50 % with all available genomes of the genus Paracoccus in the ncbi database.
31674893	10	38	dep	strains	1279:1285	arg1	2251T					1288:1292	2251T	1288:1292	2251T	1288:1292	Both strains (2251T and 3058) had DNA-DNA relatedness values less than 50 % with all available genomes of the genus Paracoccus in the ncbi database.
31674893	3	39	theme	rRNA	344:347	arg1	sequences					354:362	16S rRNA gene sequences	340:362	16S rRNA gene sequences	340:362	The results of phylogenetic analyses of 16S rRNA gene sequences indicated that strains 2251T and 3058 represent a new species within the genus Paracoccus and are most similar to 'Paracoccus gahaiensis' CUG00006T (98.9 and 99.3 %), Paracoccus nototheniae I-41R45T (98.3 and 98.7 %) and Paracoccus hibiscisoli THG-T2.31T (97.6 and 97.8 %).
31674893	4	40	theme	genomic	649:655	arg1	analysis					685:692	genomic sequence-based phylogenomic analysis	649:692	genomic sequence-based phylogenomic analysis	649:692	Results of genomic sequence-based phylogenomic analysis agreed with those from 16S rRNA gene sequence analysis.
31674893	11	41	theme	name	1636:1639	arg1	sp					1666:1667	the name Paracoccus liaowanqingii sp	1632:1667	the name Paracoccus liaowanqingii sp	1632:1667	Differential genotypic inferences, together with phenotypic and biochemical characteristics, demonstrated that strains 2251T and 3058 should be classified as a novel species of the genus Paracoccus, for which the name Paracoccus liaowanqingii sp.
31674893	4	42	theme	analysis	685:692	arg1	Results					638:644	Results	638:644	Results of genomic sequence-based phylogenomic analysis	638:692	Results of genomic sequence-based phylogenomic analysis agreed with those from 16S rRNA gene sequence analysis.
31674893	11	43	theme	phenotypic	1472:1481	arg1	characteristics					1499:1513	phenotypic and biochemical characteristics	1472:1513	phenotypic and biochemical characteristics	1472:1513	Differential genotypic inferences, together with phenotypic and biochemical characteristics, demonstrated that strains 2251T and 3058 should be classified as a novel species of the genus Paracoccus, for which the name Paracoccus liaowanqingii sp.
31674893	3	44	theme	gene	349:352	arg1	sequences					354:362	16S rRNA gene sequences	340:362	16S rRNA gene sequences	340:362	The results of phylogenetic analyses of 16S rRNA gene sequences indicated that strains 2251T and 3058 represent a new species within the genus Paracoccus and are most similar to 'Paracoccus gahaiensis' CUG00006T (98.9 and 99.3 %), Paracoccus nototheniae I-41R45T (98.3 and 98.7 %) and Paracoccus hibiscisoli THG-T2.31T (97.6 and 97.8 %).
31674893	6	45	theme	cellular	863:870	arg1	ω7c					846:848	C18 : 1 ω7c	838:848	C18 : 1 ω7c	838:848	Cells contained C18 : 1 ω7c as the major cellular fatty acid and ubiquinone-10 as the predominant menaquinone.
31674893	6	45	theme	cellular	863:870	arg1	acid					878:881	the major cellular fatty acid	853:881	the major cellular fatty acid	853:881	Cells contained C18 : 1 ω7c as the major cellular fatty acid and ubiquinone-10 as the predominant menaquinone.
31674893	2	46	theme	Gram-stain-negative	149:167	arg1	bacilli					210:216	aerobic, Gram-stain-negative, non-motile, coccoid or short rod-shaped bacilli	140:216	aerobic, Gram-stain-negative, non-motile, coccoid or short rod-shaped bacilli	140:216	Two strains, designated 2251T and 3058, that were aerobic, Gram-stain-negative, non-motile, coccoid or short rod-shaped bacilli, have recently been isolated from Tibetan antelopes on the Qinghai-Tibet Plateau.
31674893	4	47	theme	phylogenomic	672:683	arg1	analysis					685:692	genomic sequence-based phylogenomic analysis	649:692	genomic sequence-based phylogenomic analysis	649:692	Results of genomic sequence-based phylogenomic analysis agreed with those from 16S rRNA gene sequence analysis.
31674893	3	48	theme	genus	437:441	arg1	Paracoccus					443:452	the genus Paracoccus	433:452	the genus Paracoccus	433:452	The results of phylogenetic analyses of 16S rRNA gene sequences indicated that strains 2251T and 3058 represent a new species within the genus Paracoccus and are most similar to 'Paracoccus gahaiensis' CUG00006T (98.9 and 99.3 %), Paracoccus nototheniae I-41R45T (98.3 and 98.7 %) and Paracoccus hibiscisoli THG-T2.31T (97.6 and 97.8 %).
31674893	6	49	theme	major	857:861	arg1	ω7c					846:848	C18 : 1 ω7c	838:848	C18 : 1 ω7c	838:848	Cells contained C18 : 1 ω7c as the major cellular fatty acid and ubiquinone-10 as the predominant menaquinone.
31674893	6	49	theme	major	857:861	arg1	acid					878:881	the major cellular fatty acid	853:881	the major cellular fatty acid	853:881	Cells contained C18 : 1 ω7c as the major cellular fatty acid and ubiquinone-10 as the predominant menaquinone.
31674893	10	50	theme	ncbi	1408:1411	arg1	database					1413:1420	the ncbi database	1404:1420	the ncbi database	1404:1420	Both strains (2251T and 3058) had DNA-DNA relatedness values less than 50 % with all available genomes of the genus Paracoccus in the ncbi database.
31674893	8	51	theme	glutamic	1171:1178	arg1	acid					1180:1183	glutamic acid	1171:1183	glutamic acid	1171:1183	The cell-wall peptidoglycan amino acids were meso-2,6-diaminopimelic acid, alanine and glutamic acid; the major cell-wall sugar was galactose.
31674893	11	52	dep	Paracoccus	1641:1650	arg1	liaowanqingii					1652:1664	liaowanqingii	1652:1664	liaowanqingii	1652:1664	Differential genotypic inferences, together with phenotypic and biochemical characteristics, demonstrated that strains 2251T and 3058 should be classified as a novel species of the genus Paracoccus, for which the name Paracoccus liaowanqingii sp.
31674893	2	53	theme	aerobic	140:146	arg1	bacilli					210:216	aerobic, Gram-stain-negative, non-motile, coccoid or short rod-shaped bacilli	140:216	aerobic, Gram-stain-negative, non-motile, coccoid or short rod-shaped bacilli	140:216	Two strains, designated 2251T and 3058, that were aerobic, Gram-stain-negative, non-motile, coccoid or short rod-shaped bacilli, have recently been isolated from Tibetan antelopes on the Qinghai-Tibet Plateau.
31674893	2	54	theme	rod-shaped	199:208	arg1	bacilli					210:216	aerobic, Gram-stain-negative, non-motile, coccoid or short rod-shaped bacilli	140:216	aerobic, Gram-stain-negative, non-motile, coccoid or short rod-shaped bacilli	140:216	Two strains, designated 2251T and 3058, that were aerobic, Gram-stain-negative, non-motile, coccoid or short rod-shaped bacilli, have recently been isolated from Tibetan antelopes on the Qinghai-Tibet Plateau.
31674893	5	55	theme	Optimal	750:756	arg1	growth					758:763	Optimal growth	750:763	Optimal growth	750:763	Optimal growth was achieved at pH 7.0-7.5 and 28 °C with marine medium.
31674893	10	56	from	Paracoccus	1390:1399	arg1	database					1413:1420	the ncbi database	1404:1420	the ncbi database	1404:1420	Both strains (2251T and 3058) had DNA-DNA relatedness values less than 50 % with all available genomes of the genus Paracoccus in the ncbi database.
31674893	6	57	theme	fatty	872:876	arg1	ω7c					846:848	C18 : 1 ω7c	838:848	C18 : 1 ω7c	838:848	Cells contained C18 : 1 ω7c as the major cellular fatty acid and ubiquinone-10 as the predominant menaquinone.
31674893	6	57	theme	fatty	872:876	arg1	acid					878:881	the major cellular fatty acid	853:881	the major cellular fatty acid	853:881	Cells contained C18 : 1 ω7c as the major cellular fatty acid and ubiquinone-10 as the predominant menaquinone.
31674893	2	58	theme	short	193:197	arg1	bacilli					210:216	aerobic, Gram-stain-negative, non-motile, coccoid or short rod-shaped bacilli	140:216	aerobic, Gram-stain-negative, non-motile, coccoid or short rod-shaped bacilli	140:216	Two strains, designated 2251T and 3058, that were aerobic, Gram-stain-negative, non-motile, coccoid or short rod-shaped bacilli, have recently been isolated from Tibetan antelopes on the Qinghai-Tibet Plateau.
31674893	10	59	from	genomes	1369:1375	arg1	database					1413:1420	the ncbi database	1404:1420	the ncbi database	1404:1420	Both strains (2251T and 3058) had DNA-DNA relatedness values less than 50 % with all available genomes of the genus Paracoccus in the ncbi database.
31674893	9	60	theme	strain	1246:1251	arg1	2251T					1253:1257	strain 2251T	1246:1257	strain 2251T	1246:1257	The G+C content of strain 2251T was 66.5 mol%.
31674893	2	61	attach	isolated	238:245	arg2	strains					94:100	Two strains	90:100	Two strains	90:100	Two strains, designated 2251T and 3058, that were aerobic, Gram-stain-negative, non-motile, coccoid or short rod-shaped bacilli, have recently been isolated from Tibetan antelopes on the Qinghai-Tibet Plateau.
31674893	2	61	attach	isolated	238:245	arg1	antelopes					260:268	Tibetan antelopes	252:268	Tibetan antelopes	252:268	Two strains, designated 2251T and 3058, that were aerobic, Gram-stain-negative, non-motile, coccoid or short rod-shaped bacilli, have recently been isolated from Tibetan antelopes on the Qinghai-Tibet Plateau.
31674893	11	62	theme	novel	1583:1587	arg1	species					1589:1595	a novel species	1581:1595	a novel species	1581:1595	Differential genotypic inferences, together with phenotypic and biochemical characteristics, demonstrated that strains 2251T and 3058 should be classified as a novel species of the genus Paracoccus, for which the name Paracoccus liaowanqingii sp.
31674893	11	62	theme	novel	1583:1587	arg1	strains					1534:1540	strains 2251T and 3058	1534:1555	strains	1534:1540	Differential genotypic inferences, together with phenotypic and biochemical characteristics, demonstrated that strains 2251T and 3058 should be classified as a novel species of the genus Paracoccus, for which the name Paracoccus liaowanqingii sp.
31674893	11	62	theme	novel	1583:1587	arg1	3058					1552:1555	3058	1552:1555	3058	1552:1555	Differential genotypic inferences, together with phenotypic and biochemical characteristics, demonstrated that strains 2251T and 3058 should be classified as a novel species of the genus Paracoccus, for which the name Paracoccus liaowanqingii sp.
31674893	2	63	theme	coccoid	182:188	arg1	bacilli					210:216	aerobic, Gram-stain-negative, non-motile, coccoid or short rod-shaped bacilli	140:216	aerobic, Gram-stain-negative, non-motile, coccoid or short rod-shaped bacilli	140:216	Two strains, designated 2251T and 3058, that were aerobic, Gram-stain-negative, non-motile, coccoid or short rod-shaped bacilli, have recently been isolated from Tibetan antelopes on the Qinghai-Tibet Plateau.
31674893	13	64	theme	type	1693:1696	arg1	2251T					1708:1712	2251T	1708:1712	2251T (=CGMCC 1.16490T=DSM 106269T)	1708:1742	The type strain is 2251T (=CGMCC 1.16490T=DSM 106269T).
31674893	13	64	theme	type	1693:1696	arg1	strain					1698:1703	The type strain	1689:1703	The type strain	1689:1703	The type strain is 2251T (=CGMCC 1.16490T=DSM 106269T).
31674893	11	65	dep	together	1458:1465	arg1	with					1467:1470	with	1467:1470	with	1467:1470	Differential genotypic inferences, together with phenotypic and biochemical characteristics, demonstrated that strains 2251T and 3058 should be classified as a novel species of the genus Paracoccus, for which the name Paracoccus liaowanqingii sp.
31674893	10	66	contain	had	1304:1306	arg1	strains					1279:1285	Both strains	1274:1285	Both strains (2251T and 3058)	1274:1302	Both strains (2251T and 3058) had DNA-DNA relatedness values less than 50 % with all available genomes of the genus Paracoccus in the ncbi database.
31674893	10	66	contain	had	1304:1306	arg2	values					1328:1333	DNA-DNA relatedness values	1308:1333	DNA-DNA relatedness values less than 50 % with all available genomes of the genus Paracoccus in the ncbi database	1308:1420	Both strains (2251T and 3058) had DNA-DNA relatedness values less than 50 % with all available genomes of the genus Paracoccus in the ncbi database.
31674893	7	67	theme	polar	937:941	arg1	lipids					943:948	The polar lipids	933:948	The polar lipids	933:948	The polar lipids comprised phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phospholipid, glycolipid and an unidentified lipid.
31674893	4	68	theme	rRNA	721:724	arg1	analysis					740:747	16S rRNA gene sequence analysis	717:747	16S rRNA gene sequence analysis	717:747	Results of genomic sequence-based phylogenomic analysis agreed with those from 16S rRNA gene sequence analysis.
31674893	11	69	theme	Paracoccus	1641:1650	arg1	sp					1666:1667	the name Paracoccus liaowanqingii sp	1632:1667	the name Paracoccus liaowanqingii sp	1632:1667	Differential genotypic inferences, together with phenotypic and biochemical characteristics, demonstrated that strains 2251T and 3058 should be classified as a novel species of the genus Paracoccus, for which the name Paracoccus liaowanqingii sp.
31674893	3	70	theme	Paracoccus	585:594	arg1	%					634:634	97.6 and 97.8 %	620:634	%	634:634	The results of phylogenetic analyses of 16S rRNA gene sequences indicated that strains 2251T and 3058 represent a new species within the genus Paracoccus and are most similar to 'Paracoccus gahaiensis' CUG00006T (98.9 and 99.3 %), Paracoccus nototheniae I-41R45T (98.3 and 98.7 %) and Paracoccus hibiscisoli THG-T2.31T (97.6 and 97.8 %).
31674893	3	70	theme	Paracoccus	585:594	arg1	THG-T2.31T					608:617	Paracoccus hibiscisoli THG-T2.31T	585:617	Paracoccus hibiscisoli THG-T2.31T (97.6 and 97.8 %)	585:635	The results of phylogenetic analyses of 16S rRNA gene sequences indicated that strains 2251T and 3058 represent a new species within the genus Paracoccus and are most similar to 'Paracoccus gahaiensis' CUG00006T (98.9 and 99.3 %), Paracoccus nototheniae I-41R45T (98.3 and 98.7 %) and Paracoccus hibiscisoli THG-T2.31T (97.6 and 97.8 %).
31674893	4	71	theme	16S	717:719	arg1	analysis					740:747	16S rRNA gene sequence analysis	717:747	16S rRNA gene sequence analysis	717:747	Results of genomic sequence-based phylogenomic analysis agreed with those from 16S rRNA gene sequence analysis.
31674893	5	72	theme	marine	807:812	arg1	medium					814:819	marine medium	807:819	marine medium	807:819	Optimal growth was achieved at pH 7.0-7.5 and 28 °C with marine medium.
31674893	13	73	theme	1.16490T=DSM	1722:1733	arg1	106269T					1735:1741	=CGMCC 1.16490T=DSM 106269T	1715:1741	=CGMCC 1.16490T=DSM 106269T	1715:1741	The type strain is 2251T (=CGMCC 1.16490T=DSM 106269T).
31674893	13	73	theme	1.16490T=DSM	1722:1733	arg1	2251T					1708:1712	2251T	1708:1712	2251T (=CGMCC 1.16490T=DSM 106269T)	1708:1742	The type strain is 2251T (=CGMCC 1.16490T=DSM 106269T).
31674893	3	74	theme	hibiscisoli	596:606	arg1	%					634:634	97.6 and 97.8 %	620:634	%	634:634	The results of phylogenetic analyses of 16S rRNA gene sequences indicated that strains 2251T and 3058 represent a new species within the genus Paracoccus and are most similar to 'Paracoccus gahaiensis' CUG00006T (98.9 and 99.3 %), Paracoccus nototheniae I-41R45T (98.3 and 98.7 %) and Paracoccus hibiscisoli THG-T2.31T (97.6 and 97.8 %).
31674893	3	74	theme	hibiscisoli	596:606	arg1	THG-T2.31T					608:617	Paracoccus hibiscisoli THG-T2.31T	585:617	Paracoccus hibiscisoli THG-T2.31T (97.6 and 97.8 %)	585:635	The results of phylogenetic analyses of 16S rRNA gene sequences indicated that strains 2251T and 3058 represent a new species within the genus Paracoccus and are most similar to 'Paracoccus gahaiensis' CUG00006T (98.9 and 99.3 %), Paracoccus nototheniae I-41R45T (98.3 and 98.7 %) and Paracoccus hibiscisoli THG-T2.31T (97.6 and 97.8 %).
31674893	4	75	theme	sequence	731:738	arg1	analysis					740:747	16S rRNA gene sequence analysis	717:747	16S rRNA gene sequence analysis	717:747	Results of genomic sequence-based phylogenomic analysis agreed with those from 16S rRNA gene sequence analysis.
31674893	6	76	contain	contained	828:836	arg2	acid					878:881	the major cellular fatty acid	853:881	the major cellular fatty acid	853:881	Cells contained C18 : 1 ω7c as the major cellular fatty acid and ubiquinone-10 as the predominant menaquinone.
31674893	6	76	contain	contained	828:836	arg2	menaquinone					920:930	the predominant menaquinone	904:930	the predominant menaquinone	904:930	Cells contained C18 : 1 ω7c as the major cellular fatty acid and ubiquinone-10 as the predominant menaquinone.
31674893	6	76	contain	contained	828:836	arg2	ω7c					846:848	C18 : 1 ω7c	838:848	C18 : 1 ω7c	838:848	Cells contained C18 : 1 ω7c as the major cellular fatty acid and ubiquinone-10 as the predominant menaquinone.
31674893	6	76	contain	contained	828:836	arg1	Cells					822:826	Cells	822:826	Cells	822:826	Cells contained C18 : 1 ω7c as the major cellular fatty acid and ubiquinone-10 as the predominant menaquinone.
31674893	10	77	with	%	1348:1348	arg1	genomes					1369:1375	all available genomes	1355:1375	all available genomes of the genus Paracoccus in the ncbi database	1355:1420	Both strains (2251T and 3058) had DNA-DNA relatedness values less than 50 % with all available genomes of the genus Paracoccus in the ncbi database.
31674893	3	78	theme	97.8 	629:633	arg1	%					634:634	97.6 and 97.8 %	620:634	%	634:634	The results of phylogenetic analyses of 16S rRNA gene sequences indicated that strains 2251T and 3058 represent a new species within the genus Paracoccus and are most similar to 'Paracoccus gahaiensis' CUG00006T (98.9 and 99.3 %), Paracoccus nototheniae I-41R45T (98.3 and 98.7 %) and Paracoccus hibiscisoli THG-T2.31T (97.6 and 97.8 %).
31674893	3	78	theme	97.8 	629:633	arg1	THG-T2.31T					608:617	Paracoccus hibiscisoli THG-T2.31T	585:617	Paracoccus hibiscisoli THG-T2.31T (97.6 and 97.8 %)	585:635	The results of phylogenetic analyses of 16S rRNA gene sequences indicated that strains 2251T and 3058 represent a new species within the genus Paracoccus and are most similar to 'Paracoccus gahaiensis' CUG00006T (98.9 and 99.3 %), Paracoccus nototheniae I-41R45T (98.3 and 98.7 %) and Paracoccus hibiscisoli THG-T2.31T (97.6 and 97.8 %).
31674893	4	79	theme	gene	726:729	arg1	analysis					740:747	16S rRNA gene sequence analysis	717:747	16S rRNA gene sequence analysis	717:747	Results of genomic sequence-based phylogenomic analysis agreed with those from 16S rRNA gene sequence analysis.
31674893	3	80	theme	sequences	354:362	arg1	analyses					328:335	phylogenetic analyses	315:335	phylogenetic analyses of 16S rRNA gene sequences	315:362	The results of phylogenetic analyses of 16S rRNA gene sequences indicated that strains 2251T and 3058 represent a new species within the genus Paracoccus and are most similar to 'Paracoccus gahaiensis' CUG00006T (98.9 and 99.3 %), Paracoccus nototheniae I-41R45T (98.3 and 98.7 %) and Paracoccus hibiscisoli THG-T2.31T (97.6 and 97.8 %).
31674893	6	81	theme	predominant	908:918	arg1	menaquinone					920:930	the predominant menaquinone	904:930	the predominant menaquinone	904:930	Cells contained C18 : 1 ω7c as the major cellular fatty acid and ubiquinone-10 as the predominant menaquinone.
31674893	6	81	theme	predominant	908:918	arg1	ω7c					846:848	C18 : 1 ω7c	838:848	C18 : 1 ω7c	838:848	Cells contained C18 : 1 ω7c as the major cellular fatty acid and ubiquinone-10 as the predominant menaquinone.
34443442	7	0	dep	monounsaturated	1183:1197	arg1	g					1208:1208	4 g/100 g	1200:1208	4 g/100 g	1200:1208	The lipid profile showed that the flour contained monounsaturated (4 g/100 g) and polyunsaturated (46 g/100 g) fatty acids.
34443442	12	1	dep	it	2190:2191	arg1	ingredient					2215:2224	ingredient	2215:2224	ingredient	2215:2224	These results demonstrate that jackfruit seed flour has good nutritional value and antioxidant and prebiotic activity, as well as potential protective effects and functional properties, making it an attractive food or ingredient in developing innovative functional products.
34443442	12	1	dep	it	2190:2191	arg1	food					2207:2210	an attractive food	2193:2210	an attractive food	2193:2210	These results demonstrate that jackfruit seed flour has good nutritional value and antioxidant and prebiotic activity, as well as potential protective effects and functional properties, making it an attractive food or ingredient in developing innovative functional products.
34443442	11	2	contain	had	1849:1851	arg1	flour					1843:1847	this flour	1838:1847	this flour	1838:1847	Additionally, this flour had a protective and preventive effect which is associated with the potential prebiotic activity in Lactobacillus casei and Bifidobacterium longum.
34443442	11	2	contain	had	1849:1851	arg2	effect					1881:1886	a protective and preventive effect	1853:1886	a protective and preventive effect which is associated with the potential prebiotic activity in Lactobacillus casei and Bifidobacterium longum	1853:1994	Additionally, this flour had a protective and preventive effect which is associated with the potential prebiotic activity in Lactobacillus casei and Bifidobacterium longum.
34443442	9	3	theme	antioxidant	1495:1505	arg1	capacity					1507:1514	the antioxidant capacity	1491:1514	the antioxidant capacity obtained by ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH)	1491:1608	The total polyphenol content was 2.42 mg of gallic acid/g of the sample; furthermore, the antioxidant capacity obtained by ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 901.45 µmol Trolox/100 g and 1607.87 µmol Trolox/100 g, respectively.
34443442	9	3	theme	antioxidant	1495:1505	arg1	g					1637:1637	901.45 µmol Trolox/100 g	1614:1637	901.45 µmol Trolox/100 g	1614:1637	The total polyphenol content was 2.42 mg of gallic acid/g of the sample; furthermore, the antioxidant capacity obtained by ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 901.45 µmol Trolox/100 g and 1607.87 µmol Trolox/100 g, respectively.
34443442	3	4	from	temperatures	628:639	arg1	drying					608:613	jackfruit seed drying	593:613	jackfruit seed drying at different temperatures	593:639	The experimental values of the moisture ratio during jackfruit seed drying at different temperatures were obtained using Page's equation to establish the drying time for the required moisture between 5 and 7% in the flour.
34443442	10	5	theme	oil	1759:1761	arg1	capacity					1774:1781	oil absorption capacity	1759:1781	oil absorption capacity	1759:1781	The obtained flour exhibited good functional properties, such as water and oil absorption capacity, swelling power and emulsifier capacity.
34443442	11	6	theme	prebiotic	1927:1935	arg1	activity					1937:1944	the potential prebiotic activity	1913:1944	the potential prebiotic activity in Lactobacillus casei and Bifidobacterium longum	1913:1994	Additionally, this flour had a protective and preventive effect which is associated with the potential prebiotic activity in Lactobacillus casei and Bifidobacterium longum.
34443442	11	7	theme	preventive	1870:1879	arg1	effect					1881:1886	a protective and preventive effect	1853:1886	a protective and preventive effect which is associated with the potential prebiotic activity in Lactobacillus casei and Bifidobacterium longum	1853:1994	Additionally, this flour had a protective and preventive effect which is associated with the potential prebiotic activity in Lactobacillus casei and Bifidobacterium longum.
34443442	2	8	theme	antioxidant	518:528	arg1	capacity					530:537	antioxidant capacity	518:537	antioxidant capacity	518:537	The drying processes of jackfruit seeds were performed at 50, 60 and 70 °C in order to choose the optimal temperature for obtaining the flour based on drying time, polyphenol content and antioxidant capacity.
34443442	7	9	theme	fatty	1244:1248	arg1	acids					1250:1254	monounsaturated (4 g/100 g) and polyunsaturated (46 g/100 g) fatty acids	1183:1254	monounsaturated (4 g/100 g) and polyunsaturated (46 g/100 g) fatty acids	1183:1254	The lipid profile showed that the flour contained monounsaturated (4 g/100 g) and polyunsaturated (46 g/100 g) fatty acids.
34443442	10	10	theme	swelling	1784:1791	arg1	power					1793:1797	swelling power	1784:1797	swelling power	1784:1797	The obtained flour exhibited good functional properties, such as water and oil absorption capacity, swelling power and emulsifier capacity.
34443442	11	11	theme	protective	1855:1864	arg1	effect					1881:1886	a protective and preventive effect	1853:1886	a protective and preventive effect which is associated with the potential prebiotic activity in Lactobacillus casei and Bifidobacterium longum	1853:1994	Additionally, this flour had a protective and preventive effect which is associated with the potential prebiotic activity in Lactobacillus casei and Bifidobacterium longum.
34443442	9	12	theme	antioxidant	1544:1554	arg1	FRAP					1563:1566	FRAP	1563:1566	FRAP	1563:1566	The total polyphenol content was 2.42 mg of gallic acid/g of the sample; furthermore, the antioxidant capacity obtained by ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 901.45 µmol Trolox/100 g and 1607.87 µmol Trolox/100 g, respectively.
34443442	9	12	theme	antioxidant	1544:1554	arg1	power					1556:1560	ferric reducing antioxidant power	1528:1560	ferric reducing antioxidant power (FRAP)	1528:1567	The total polyphenol content was 2.42 mg of gallic acid/g of the sample; furthermore, the antioxidant capacity obtained by ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 901.45 µmol Trolox/100 g and 1607.87 µmol Trolox/100 g, respectively.
34443442	0	13	from	Potential	84:92	arg1	Cells					103:107	Human Cells	97:107	Human Cells of Jackfruit (Artocarpus heterophyllus)	97:147	Physicochemical Properties, Antioxidant Capacity, Prebiotic Activity and Anticancer Potential in Human Cells of Jackfruit (Artocarpus heterophyllus) Seed Flour.
34443442	11	14	from	activity	1937:1944	arg1	casei					1963:1967	Lactobacillus casei	1949:1967	Lactobacillus casei	1949:1967	Additionally, this flour had a protective and preventive effect which is associated with the potential prebiotic activity in Lactobacillus casei and Bifidobacterium longum.
34443442	11	14	from	activity	1937:1944	arg1	longum					1989:1994	Bifidobacterium longum	1973:1994	Bifidobacterium longum	1973:1994	Additionally, this flour had a protective and preventive effect which is associated with the potential prebiotic activity in Lactobacillus casei and Bifidobacterium longum.
34443442	3	15	theme	moisture	571:578	arg1	ratio					580:584	the moisture ratio	567:584	the moisture ratio during jackfruit seed drying at different temperatures	567:639	The experimental values of the moisture ratio during jackfruit seed drying at different temperatures were obtained using Page's equation to establish the drying time for the required moisture between 5 and 7% in the flour.
34443442	11	16	theme	potential	1917:1925	arg1	activity					1937:1944	the potential prebiotic activity	1913:1944	the potential prebiotic activity in Lactobacillus casei and Bifidobacterium longum	1913:1994	Additionally, this flour had a protective and preventive effect which is associated with the potential prebiotic activity in Lactobacillus casei and Bifidobacterium longum.
34443442	12	17	theme	attractive	2196:2205	arg1	food					2207:2210	an attractive food	2193:2210	an attractive food	2193:2210	These results demonstrate that jackfruit seed flour has good nutritional value and antioxidant and prebiotic activity, as well as potential protective effects and functional properties, making it an attractive food or ingredient in developing innovative functional products.
34443442	0	18	theme	Seed	149:152	arg1	Flour					154:158	Seed Flour	149:158	Seed Flour	149:158	Physicochemical Properties, Antioxidant Capacity, Prebiotic Activity and Anticancer Potential in Human Cells of Jackfruit (Artocarpus heterophyllus) Seed Flour.
34443442	12	19	theme	functional	2160:2169	arg1	properties					2171:2180	functional properties	2160:2180	functional properties	2160:2180	These results demonstrate that jackfruit seed flour has good nutritional value and antioxidant and prebiotic activity, as well as potential protective effects and functional properties, making it an attractive food or ingredient in developing innovative functional products.
34443442	7	20	theme	polyunsaturated	1215:1229	arg1	acids					1250:1254	monounsaturated (4 g/100 g) and polyunsaturated (46 g/100 g) fatty acids	1183:1254	monounsaturated (4 g/100 g) and polyunsaturated (46 g/100 g) fatty acids	1183:1254	The lipid profile showed that the flour contained monounsaturated (4 g/100 g) and polyunsaturated (46 g/100 g) fatty acids.
34443442	3	21	theme	seed	603:606	arg1	drying					608:613	jackfruit seed drying	593:613	jackfruit seed drying at different temperatures	593:639	The experimental values of the moisture ratio during jackfruit seed drying at different temperatures were obtained using Page's equation to establish the drying time for the required moisture between 5 and 7% in the flour.
34443442	5	22	theme	chemical	882:889	arg1	composition					891:901	The chemical composition	878:901	The chemical composition	878:901	The chemical composition, total dietary fiber, functional properties and antioxidant capacity were then examined in the flour.
34443442	8	23	theme	predominant	1309:1319	arg1	glucose					1266:1272	glucose	1266:1272	glucose	1266:1272	Sucrose, glucose, and fructose were found to be the predominant soluble sugars, and non-digestible oligosaccharides like 1-kestose were also found.
34443442	8	23	theme	predominant	1309:1319	arg1	sugars					1329:1334	the predominant soluble sugars	1305:1334	the predominant soluble sugars	1305:1334	Sucrose, glucose, and fructose were found to be the predominant soluble sugars, and non-digestible oligosaccharides like 1-kestose were also found.
34443442	8	23	theme	predominant	1309:1319	arg1	Sucrose					1257:1263	Sucrose	1257:1263	Sucrose	1257:1263	Sucrose, glucose, and fructose were found to be the predominant soluble sugars, and non-digestible oligosaccharides like 1-kestose were also found.
34443442	8	23	theme	predominant	1309:1319	arg1	fructose					1279:1286	fructose	1279:1286	fructose	1279:1286	Sucrose, glucose, and fructose were found to be the predominant soluble sugars, and non-digestible oligosaccharides like 1-kestose were also found.
34443442	6	24	contain	contains	1020:1027	arg2	g					1056:1056	73.87 g/100 g	1044:1056	73.87 g/100 g	1044:1056	The seed flour contains carbohydrates (73.87 g/100 g), dietary fiber (31 g/100 g), protein (14 g/100 g) and lipids (1 g/100 g).
34443442	6	24	contain	contains	1020:1027	arg2	carbohydrates					1029:1041	carbohydrates	1029:1041	carbohydrates (73.87 g/100 g)	1029:1057	The seed flour contains carbohydrates (73.87 g/100 g), dietary fiber (31 g/100 g), protein (14 g/100 g) and lipids (1 g/100 g).
34443442	6	24	contain	contains	1020:1027	arg2	g					1106:1106	14 g/100 g	1097:1106	14 g/100 g	1097:1106	The seed flour contains carbohydrates (73.87 g/100 g), dietary fiber (31 g/100 g), protein (14 g/100 g) and lipids (1 g/100 g).
34443442	6	24	contain	contains	1020:1027	arg2	g					1084:1084	31 g/100 g	1075:1084	31 g/100 g	1075:1084	The seed flour contains carbohydrates (73.87 g/100 g), dietary fiber (31 g/100 g), protein (14 g/100 g) and lipids (1 g/100 g).
34443442	6	24	contain	contains	1020:1027	arg2	protein					1088:1094	protein	1088:1094	protein (14 g/100 g)	1088:1107	The seed flour contains carbohydrates (73.87 g/100 g), dietary fiber (31 g/100 g), protein (14 g/100 g) and lipids (1 g/100 g).
34443442	6	24	contain	contains	1020:1027	arg2	fiber					1068:1072	dietary fiber	1060:1072	dietary fiber (31 g/100 g)	1060:1085	The seed flour contains carbohydrates (73.87 g/100 g), dietary fiber (31 g/100 g), protein (14 g/100 g) and lipids (1 g/100 g).
34443442	6	24	contain	contains	1020:1027	arg1	flour					1014:1018	The seed flour	1005:1018	The seed flour	1005:1018	The seed flour contains carbohydrates (73.87 g/100 g), dietary fiber (31 g/100 g), protein (14 g/100 g) and lipids (1 g/100 g).
34443442	6	24	contain	contains	1020:1027	arg2	g					1129:1129	1 g/100 g	1121:1129	1 g/100 g	1121:1129	The seed flour contains carbohydrates (73.87 g/100 g), dietary fiber (31 g/100 g), protein (14 g/100 g) and lipids (1 g/100 g).
34443442	6	24	contain	contains	1020:1027	arg2	lipids					1113:1118	lipids	1113:1118	lipids (1 g/100 g)	1113:1130	The seed flour contains carbohydrates (73.87 g/100 g), dietary fiber (31 g/100 g), protein (14 g/100 g) and lipids (1 g/100 g).
34443442	0	25	theme	Human	97:101	arg1	Cells					103:107	Human Cells	97:107	Human Cells of Jackfruit (Artocarpus heterophyllus)	97:147	Physicochemical Properties, Antioxidant Capacity, Prebiotic Activity and Anticancer Potential in Human Cells of Jackfruit (Artocarpus heterophyllus) Seed Flour.
34443442	2	26	theme	drying	482:487	arg1	time					489:492	drying time	482:492	drying time	482:492	The drying processes of jackfruit seeds were performed at 50, 60 and 70 °C in order to choose the optimal temperature for obtaining the flour based on drying time, polyphenol content and antioxidant capacity.
34443442	1	27	dep	flour	324:328	arg1	heterophyllus					304:316	Artocarpus heterophyllus	293:316	Artocarpus heterophyllus	293:316	The general aim of this study was to evaluate physicochemical properties, prebiotic activity and anticancer potential of jackfruit (Artocarpus heterophyllus) seed flour.
34443442	0	28	from	Capacity	40:47	arg1	Cells					103:107	Human Cells	97:107	Human Cells of Jackfruit (Artocarpus heterophyllus)	97:147	Physicochemical Properties, Antioxidant Capacity, Prebiotic Activity and Anticancer Potential in Human Cells of Jackfruit (Artocarpus heterophyllus) Seed Flour.
34443442	12	29	theme	nutritional	2058:2068	arg1	value					2070:2074	good nutritional value	2053:2074	good nutritional value	2053:2074	These results demonstrate that jackfruit seed flour has good nutritional value and antioxidant and prebiotic activity, as well as potential protective effects and functional properties, making it an attractive food or ingredient in developing innovative functional products.
34443442	0	30	theme	Physicochemical	0:14	arg1	Properties					16:25	Physicochemical Properties	0:25	Physicochemical Properties	0:25	Physicochemical Properties, Antioxidant Capacity, Prebiotic Activity and Anticancer Potential in Human Cells of Jackfruit (Artocarpus heterophyllus) Seed Flour.
34443442	9	31	theme	polyphenol	1415:1424	arg1	content					1426:1432	The total polyphenol content	1405:1432	The total polyphenol content	1405:1432	The total polyphenol content was 2.42 mg of gallic acid/g of the sample; furthermore, the antioxidant capacity obtained by ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 901.45 µmol Trolox/100 g and 1607.87 µmol Trolox/100 g, respectively.
34443442	9	31	theme	polyphenol	1415:1424	arg1	mg					1443:1444	2.42 mg	1438:1444	2.42 mg of gallic acid/g of the sample	1438:1475	The total polyphenol content was 2.42 mg of gallic acid/g of the sample; furthermore, the antioxidant capacity obtained by ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 901.45 µmol Trolox/100 g and 1607.87 µmol Trolox/100 g, respectively.
34443442	9	32	theme	1607.87	1643:1649	arg1	g					1667:1667	1607.87 µmol Trolox/100 g	1643:1667	1607.87 µmol Trolox/100 g	1643:1667	The total polyphenol content was 2.42 mg of gallic acid/g of the sample; furthermore, the antioxidant capacity obtained by ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 901.45 µmol Trolox/100 g and 1607.87 µmol Trolox/100 g, respectively.
34443442	5	33	theme	dietary	910:916	arg1	fiber					918:922	total dietary fiber	904:922	total dietary fiber	904:922	The chemical composition, total dietary fiber, functional properties and antioxidant capacity were then examined in the flour.
34443442	7	34	theme	monounsaturated	1183:1197	arg1	acids					1250:1254	monounsaturated (4 g/100 g) and polyunsaturated (46 g/100 g) fatty acids	1183:1254	monounsaturated (4 g/100 g) and polyunsaturated (46 g/100 g) fatty acids	1183:1254	The lipid profile showed that the flour contained monounsaturated (4 g/100 g) and polyunsaturated (46 g/100 g) fatty acids.
34443442	10	35	theme	obtained	1688:1695	arg1	flour					1697:1701	The obtained flour	1684:1701	The obtained flour	1684:1701	The obtained flour exhibited good functional properties, such as water and oil absorption capacity, swelling power and emulsifier capacity.
34443442	12	36	theme	seed	2038:2041	arg1	flour					2043:2047	jackfruit seed flour	2028:2047	jackfruit seed flour	2028:2047	These results demonstrate that jackfruit seed flour has good nutritional value and antioxidant and prebiotic activity, as well as potential protective effects and functional properties, making it an attractive food or ingredient in developing innovative functional products.
34443442	2	37	theme	optimal	429:435	arg1	temperature					437:447	the optimal temperature	425:447	the optimal temperature for obtaining the flour based on drying time, polyphenol content and antioxidant capacity	425:537	The drying processes of jackfruit seeds were performed at 50, 60 and 70 °C in order to choose the optimal temperature for obtaining the flour based on drying time, polyphenol content and antioxidant capacity.
34443442	0	38	theme	Prebiotic	50:58	arg1	Activity					60:67	Prebiotic Activity	50:67	Prebiotic Activity	50:67	Physicochemical Properties, Antioxidant Capacity, Prebiotic Activity and Anticancer Potential in Human Cells of Jackfruit (Artocarpus heterophyllus) Seed Flour.
34443442	1	39	theme	seed	319:322	arg1	flour					324:328	jackfruit (Artocarpus heterophyllus) seed flour	282:328	jackfruit (Artocarpus heterophyllus) seed flour	282:328	The general aim of this study was to evaluate physicochemical properties, prebiotic activity and anticancer potential of jackfruit (Artocarpus heterophyllus) seed flour.
34443442	9	40	theme	gallic	1449:1454	arg1	acid/g					1456:1461	gallic acid/g	1449:1461	gallic acid/g of the sample	1449:1475	The total polyphenol content was 2.42 mg of gallic acid/g of the sample; furthermore, the antioxidant capacity obtained by ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 901.45 µmol Trolox/100 g and 1607.87 µmol Trolox/100 g, respectively.
34443442	10	41	theme	functional	1718:1727	arg1	water					1749:1753	water	1749:1753	water	1749:1753	The obtained flour exhibited good functional properties, such as water and oil absorption capacity, swelling power and emulsifier capacity.
34443442	10	41	theme	functional	1718:1727	arg1	capacity					1774:1781	oil absorption capacity	1759:1781	oil absorption capacity	1759:1781	The obtained flour exhibited good functional properties, such as water and oil absorption capacity, swelling power and emulsifier capacity.
34443442	10	41	theme	functional	1718:1727	arg1	properties					1729:1738	good functional properties	1713:1738	good functional properties	1713:1738	The obtained flour exhibited good functional properties, such as water and oil absorption capacity, swelling power and emulsifier capacity.
34443442	1	42	theme	physicochemical	207:221	arg1	properties					223:232	physicochemical properties	207:232	physicochemical properties	207:232	The general aim of this study was to evaluate physicochemical properties, prebiotic activity and anticancer potential of jackfruit (Artocarpus heterophyllus) seed flour.
34443442	5	43	theme	antioxidant	951:961	arg1	capacity					963:970	antioxidant capacity	951:970	antioxidant capacity	951:970	The chemical composition, total dietary fiber, functional properties and antioxidant capacity were then examined in the flour.
34443442	12	44	theme	innovative	2240:2249	arg1	products					2262:2269	innovative functional products	2240:2269	innovative functional products	2240:2269	These results demonstrate that jackfruit seed flour has good nutritional value and antioxidant and prebiotic activity, as well as potential protective effects and functional properties, making it an attractive food or ingredient in developing innovative functional products.
34443442	9	45	theme	sample	1470:1475	arg1	acid/g					1456:1461	gallic acid/g	1449:1461	gallic acid/g of the sample	1449:1475	The total polyphenol content was 2.42 mg of gallic acid/g of the sample; furthermore, the antioxidant capacity obtained by ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 901.45 µmol Trolox/100 g and 1607.87 µmol Trolox/100 g, respectively.
34443442	2	46	theme	jackfruit	355:363	arg1	seeds					365:369	jackfruit seeds	355:369	jackfruit seeds	355:369	The drying processes of jackfruit seeds were performed at 50, 60 and 70 °C in order to choose the optimal temperature for obtaining the flour based on drying time, polyphenol content and antioxidant capacity.
34443442	12	47	theme	potential	2127:2135	arg1	effects					2148:2154	potential protective effects	2127:2154	potential protective effects	2127:2154	These results demonstrate that jackfruit seed flour has good nutritional value and antioxidant and prebiotic activity, as well as potential protective effects and functional properties, making it an attractive food or ingredient in developing innovative functional products.
34443442	1	48	theme	anticancer	258:267	arg1	potential					269:277	anticancer potential	258:277	anticancer potential	258:277	The general aim of this study was to evaluate physicochemical properties, prebiotic activity and anticancer potential of jackfruit (Artocarpus heterophyllus) seed flour.
34443442	4	49	theme	good	826:829	arg1	flour					831:835	good flour	826:835	good flour	826:835	The temperature of 60 °C was considered adequate for obtaining good flour and for performing its characterization.
34443442	10	50	theme	absorption	1763:1772	arg1	capacity					1774:1781	oil absorption capacity	1759:1781	oil absorption capacity	1759:1781	The obtained flour exhibited good functional properties, such as water and oil absorption capacity, swelling power and emulsifier capacity.
34443442	1	51	theme	general	165:171	arg1	aim					173:175	The general aim	161:175	The general aim of this study	161:189	The general aim of this study was to evaluate physicochemical properties, prebiotic activity and anticancer potential of jackfruit (Artocarpus heterophyllus) seed flour.
34443442	4	52	theme	°C	785:786	arg1	temperature					767:777	The temperature	763:777	The temperature of 60 °C	763:786	The temperature of 60 °C was considered adequate for obtaining good flour and for performing its characterization.
34443442	3	53	theme	drying	694:699	arg1	time					701:704	the drying time	690:704	the drying time for the required moisture between 5 and 7% in the flour	690:760	The experimental values of the moisture ratio during jackfruit seed drying at different temperatures were obtained using Page's equation to establish the drying time for the required moisture between 5 and 7% in the flour.
34443442	8	54	theme	non-digestible	1341:1354	arg1	oligosaccharides					1356:1371	non-digestible oligosaccharides	1341:1371	non-digestible oligosaccharides like 1-kestose	1341:1386	Sucrose, glucose, and fructose were found to be the predominant soluble sugars, and non-digestible oligosaccharides like 1-kestose were also found.
34443442	1	55	theme	study	185:189	arg1	aim					173:175	The general aim	161:175	The general aim of this study	161:189	The general aim of this study was to evaluate physicochemical properties, prebiotic activity and anticancer potential of jackfruit (Artocarpus heterophyllus) seed flour.
34443442	3	56	theme	experimental	544:555	arg1	values					557:562	The experimental values	540:562	The experimental values of the moisture ratio during jackfruit seed drying at different temperatures	540:639	The experimental values of the moisture ratio during jackfruit seed drying at different temperatures were obtained using Page's equation to establish the drying time for the required moisture between 5 and 7% in the flour.
34443442	9	57	theme	reducing	1535:1542	arg1	FRAP					1563:1566	FRAP	1563:1566	FRAP	1563:1566	The total polyphenol content was 2.42 mg of gallic acid/g of the sample; furthermore, the antioxidant capacity obtained by ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 901.45 µmol Trolox/100 g and 1607.87 µmol Trolox/100 g, respectively.
34443442	9	57	theme	reducing	1535:1542	arg1	power					1556:1560	ferric reducing antioxidant power	1528:1560	ferric reducing antioxidant power (FRAP)	1528:1567	The total polyphenol content was 2.42 mg of gallic acid/g of the sample; furthermore, the antioxidant capacity obtained by ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 901.45 µmol Trolox/100 g and 1607.87 µmol Trolox/100 g, respectively.
34443442	3	58	theme	required	714:721	arg1	moisture					723:730	the required moisture	710:730	the required moisture between 5 and 7% in the flour	710:760	The experimental values of the moisture ratio during jackfruit seed drying at different temperatures were obtained using Page's equation to establish the drying time for the required moisture between 5 and 7% in the flour.
34443442	7	59	dep	polyunsaturated	1215:1229	arg1	g					1241:1241	46 g/100 g	1232:1241	46 g/100 g	1232:1241	The lipid profile showed that the flour contained monounsaturated (4 g/100 g) and polyunsaturated (46 g/100 g) fatty acids.
34443442	2	60	theme	polyphenol	495:504	arg1	content					506:512	polyphenol content	495:512	polyphenol content	495:512	The drying processes of jackfruit seeds were performed at 50, 60 and 70 °C in order to choose the optimal temperature for obtaining the flour based on drying time, polyphenol content and antioxidant capacity.
34443442	7	61	contain	contained	1173:1181	arg1	flour					1167:1171	the flour	1163:1171	the flour	1163:1171	The lipid profile showed that the flour contained monounsaturated (4 g/100 g) and polyunsaturated (46 g/100 g) fatty acids.
34443442	7	61	contain	contained	1173:1181	arg2	acids					1250:1254	monounsaturated (4 g/100 g) and polyunsaturated (46 g/100 g) fatty acids	1183:1254	monounsaturated (4 g/100 g) and polyunsaturated (46 g/100 g) fatty acids	1183:1254	The lipid profile showed that the flour contained monounsaturated (4 g/100 g) and polyunsaturated (46 g/100 g) fatty acids.
34443442	9	62	theme	ferric	1528:1533	arg1	FRAP					1563:1566	FRAP	1563:1566	FRAP	1563:1566	The total polyphenol content was 2.42 mg of gallic acid/g of the sample; furthermore, the antioxidant capacity obtained by ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 901.45 µmol Trolox/100 g and 1607.87 µmol Trolox/100 g, respectively.
34443442	9	62	theme	ferric	1528:1533	arg1	power					1556:1560	ferric reducing antioxidant power	1528:1560	ferric reducing antioxidant power (FRAP)	1528:1567	The total polyphenol content was 2.42 mg of gallic acid/g of the sample; furthermore, the antioxidant capacity obtained by ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 901.45 µmol Trolox/100 g and 1607.87 µmol Trolox/100 g, respectively.
34443442	10	63	theme	emulsifier	1803:1812	arg1	capacity					1814:1821	emulsifier capacity	1803:1821	emulsifier capacity	1803:1821	The obtained flour exhibited good functional properties, such as water and oil absorption capacity, swelling power and emulsifier capacity.
34443442	3	64	theme	jackfruit	593:601	arg1	drying					608:613	jackfruit seed drying	593:613	jackfruit seed drying at different temperatures	593:639	The experimental values of the moisture ratio during jackfruit seed drying at different temperatures were obtained using Page's equation to establish the drying time for the required moisture between 5 and 7% in the flour.
34443442	0	65	dep	Flour	154:158	arg1	Potential					84:92	Anticancer Potential	73:92	Anticancer Potential	73:92	Physicochemical Properties, Antioxidant Capacity, Prebiotic Activity and Anticancer Potential in Human Cells of Jackfruit (Artocarpus heterophyllus) Seed Flour.
34443442	0	65	dep	Flour	154:158	arg1	Properties					16:25	Physicochemical Properties	0:25	Physicochemical Properties	0:25	Physicochemical Properties, Antioxidant Capacity, Prebiotic Activity and Anticancer Potential in Human Cells of Jackfruit (Artocarpus heterophyllus) Seed Flour.
34443442	0	65	dep	Flour	154:158	arg1	Capacity					40:47	Antioxidant Capacity	28:47	Antioxidant Capacity	28:47	Physicochemical Properties, Antioxidant Capacity, Prebiotic Activity and Anticancer Potential in Human Cells of Jackfruit (Artocarpus heterophyllus) Seed Flour.
34443442	0	65	dep	Flour	154:158	arg1	Activity					60:67	Prebiotic Activity	50:67	Prebiotic Activity	50:67	Physicochemical Properties, Antioxidant Capacity, Prebiotic Activity and Anticancer Potential in Human Cells of Jackfruit (Artocarpus heterophyllus) Seed Flour.
34443442	8	66	theme	soluble	1321:1327	arg1	glucose					1266:1272	glucose	1266:1272	glucose	1266:1272	Sucrose, glucose, and fructose were found to be the predominant soluble sugars, and non-digestible oligosaccharides like 1-kestose were also found.
34443442	8	66	theme	soluble	1321:1327	arg1	sugars					1329:1334	the predominant soluble sugars	1305:1334	the predominant soluble sugars	1305:1334	Sucrose, glucose, and fructose were found to be the predominant soluble sugars, and non-digestible oligosaccharides like 1-kestose were also found.
34443442	8	66	theme	soluble	1321:1327	arg1	Sucrose					1257:1263	Sucrose	1257:1263	Sucrose	1257:1263	Sucrose, glucose, and fructose were found to be the predominant soluble sugars, and non-digestible oligosaccharides like 1-kestose were also found.
34443442	8	66	theme	soluble	1321:1327	arg1	fructose					1279:1286	fructose	1279:1286	fructose	1279:1286	Sucrose, glucose, and fructose were found to be the predominant soluble sugars, and non-digestible oligosaccharides like 1-kestose were also found.
34443442	7	67	theme	lipid	1137:1141	arg1	profile					1143:1149	The lipid profile	1133:1149	The lipid profile	1133:1149	The lipid profile showed that the flour contained monounsaturated (4 g/100 g) and polyunsaturated (46 g/100 g) fatty acids.
34443442	0	68	theme	Anticancer	73:82	arg1	Potential					84:92	Anticancer Potential	73:92	Anticancer Potential	73:92	Physicochemical Properties, Antioxidant Capacity, Prebiotic Activity and Anticancer Potential in Human Cells of Jackfruit (Artocarpus heterophyllus) Seed Flour.
34443442	3	69	theme	ratio	580:584	arg1	values					557:562	The experimental values	540:562	The experimental values of the moisture ratio during jackfruit seed drying at different temperatures	540:639	The experimental values of the moisture ratio during jackfruit seed drying at different temperatures were obtained using Page's equation to establish the drying time for the required moisture between 5 and 7% in the flour.
34443442	12	70	theme	protective	2137:2146	arg1	effects					2148:2154	potential protective effects	2127:2154	potential protective effects	2127:2154	These results demonstrate that jackfruit seed flour has good nutritional value and antioxidant and prebiotic activity, as well as potential protective effects and functional properties, making it an attractive food or ingredient in developing innovative functional products.
34443442	0	71	from	Properties	16:25	arg1	Cells					103:107	Human Cells	97:107	Human Cells of Jackfruit (Artocarpus heterophyllus)	97:147	Physicochemical Properties, Antioxidant Capacity, Prebiotic Activity and Anticancer Potential in Human Cells of Jackfruit (Artocarpus heterophyllus) Seed Flour.
34443442	0	72	dep	Jackfruit	112:120	arg1	heterophyllus					134:146	Artocarpus heterophyllus	123:146	Artocarpus heterophyllus	123:146	Physicochemical Properties, Antioxidant Capacity, Prebiotic Activity and Anticancer Potential in Human Cells of Jackfruit (Artocarpus heterophyllus) Seed Flour.
34443442	9	73	theme	901.45	1614:1619	arg1	capacity					1507:1514	the antioxidant capacity	1491:1514	the antioxidant capacity obtained by ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH)	1491:1608	The total polyphenol content was 2.42 mg of gallic acid/g of the sample; furthermore, the antioxidant capacity obtained by ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 901.45 µmol Trolox/100 g and 1607.87 µmol Trolox/100 g, respectively.
34443442	9	73	theme	901.45	1614:1619	arg1	g					1637:1637	901.45 µmol Trolox/100 g	1614:1637	901.45 µmol Trolox/100 g	1614:1637	The total polyphenol content was 2.42 mg of gallic acid/g of the sample; furthermore, the antioxidant capacity obtained by ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 901.45 µmol Trolox/100 g and 1607.87 µmol Trolox/100 g, respectively.
34443442	0	74	from	Activity	60:67	arg1	Cells					103:107	Human Cells	97:107	Human Cells of Jackfruit (Artocarpus heterophyllus)	97:147	Physicochemical Properties, Antioxidant Capacity, Prebiotic Activity and Anticancer Potential in Human Cells of Jackfruit (Artocarpus heterophyllus) Seed Flour.
34443442	3	75	from	%	747:747	arg1	flour					756:760	the flour	752:760	the flour	752:760	The experimental values of the moisture ratio during jackfruit seed drying at different temperatures were obtained using Page's equation to establish the drying time for the required moisture between 5 and 7% in the flour.
34443442	12	76	theme	antioxidant	2080:2090	arg1	activity					2106:2113	antioxidant and prebiotic activity	2080:2113	antioxidant and prebiotic activity	2080:2113	These results demonstrate that jackfruit seed flour has good nutritional value and antioxidant and prebiotic activity, as well as potential protective effects and functional properties, making it an attractive food or ingredient in developing innovative functional products.
34443442	0	77	theme	Jackfruit	112:120	arg1	Cells					103:107	Human Cells	97:107	Human Cells of Jackfruit (Artocarpus heterophyllus)	97:147	Physicochemical Properties, Antioxidant Capacity, Prebiotic Activity and Anticancer Potential in Human Cells of Jackfruit (Artocarpus heterophyllus) Seed Flour.
34443442	5	78	theme	functional	925:934	arg1	properties					936:945	functional properties	925:945	functional properties	925:945	The chemical composition, total dietary fiber, functional properties and antioxidant capacity were then examined in the flour.
34443442	12	79	theme	prebiotic	2096:2104	arg1	activity					2106:2113	antioxidant and prebiotic activity	2080:2113	antioxidant and prebiotic activity	2080:2113	These results demonstrate that jackfruit seed flour has good nutritional value and antioxidant and prebiotic activity, as well as potential protective effects and functional properties, making it an attractive food or ingredient in developing innovative functional products.
34443442	9	80	theme	µmol	1621:1624	arg1	capacity					1507:1514	the antioxidant capacity	1491:1514	the antioxidant capacity obtained by ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH)	1491:1608	The total polyphenol content was 2.42 mg of gallic acid/g of the sample; furthermore, the antioxidant capacity obtained by ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 901.45 µmol Trolox/100 g and 1607.87 µmol Trolox/100 g, respectively.
34443442	9	80	theme	µmol	1621:1624	arg1	g					1637:1637	901.45 µmol Trolox/100 g	1614:1637	901.45 µmol Trolox/100 g	1614:1637	The total polyphenol content was 2.42 mg of gallic acid/g of the sample; furthermore, the antioxidant capacity obtained by ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 901.45 µmol Trolox/100 g and 1607.87 µmol Trolox/100 g, respectively.
34443442	4	81	dep	adequate	803:810	arg1	performing					845:854	performing	845:854	performing its characterization	845:875	The temperature of 60 °C was considered adequate for obtaining good flour and for performing its characterization.
34443442	4	81	dep	adequate	803:810	arg1	obtaining					816:824	obtaining	816:824	obtaining good flour	816:835	The temperature of 60 °C was considered adequate for obtaining good flour and for performing its characterization.
34443442	9	82	theme	total	1409:1413	arg1	content					1426:1432	The total polyphenol content	1405:1432	The total polyphenol content	1405:1432	The total polyphenol content was 2.42 mg of gallic acid/g of the sample; furthermore, the antioxidant capacity obtained by ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 901.45 µmol Trolox/100 g and 1607.87 µmol Trolox/100 g, respectively.
34443442	9	82	theme	total	1409:1413	arg1	mg					1443:1444	2.42 mg	1438:1444	2.42 mg of gallic acid/g of the sample	1438:1475	The total polyphenol content was 2.42 mg of gallic acid/g of the sample; furthermore, the antioxidant capacity obtained by ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 901.45 µmol Trolox/100 g and 1607.87 µmol Trolox/100 g, respectively.
34443442	12	83	theme	good	2053:2056	arg1	value					2070:2074	good nutritional value	2053:2074	good nutritional value	2053:2074	These results demonstrate that jackfruit seed flour has good nutritional value and antioxidant and prebiotic activity, as well as potential protective effects and functional properties, making it an attractive food or ingredient in developing innovative functional products.
34443442	0	84	theme	Antioxidant	28:38	arg1	Capacity					40:47	Antioxidant Capacity	28:47	Antioxidant Capacity	28:47	Physicochemical Properties, Antioxidant Capacity, Prebiotic Activity and Anticancer Potential in Human Cells of Jackfruit (Artocarpus heterophyllus) Seed Flour.
34443442	1	85	theme	jackfruit	282:290	arg1	flour					324:328	jackfruit (Artocarpus heterophyllus) seed flour	282:328	jackfruit (Artocarpus heterophyllus) seed flour	282:328	The general aim of this study was to evaluate physicochemical properties, prebiotic activity and anticancer potential of jackfruit (Artocarpus heterophyllus) seed flour.
34443442	5	86	theme	total	904:908	arg1	fiber					918:922	total dietary fiber	904:922	total dietary fiber	904:922	The chemical composition, total dietary fiber, functional properties and antioxidant capacity were then examined in the flour.
34443442	6	87	theme	seed	1009:1012	arg1	flour					1014:1018	The seed flour	1005:1018	The seed flour	1005:1018	The seed flour contains carbohydrates (73.87 g/100 g), dietary fiber (31 g/100 g), protein (14 g/100 g) and lipids (1 g/100 g).
34443442	9	88	theme	µmol	1651:1654	arg1	g					1667:1667	1607.87 µmol Trolox/100 g	1643:1667	1607.87 µmol Trolox/100 g	1643:1667	The total polyphenol content was 2.42 mg of gallic acid/g of the sample; furthermore, the antioxidant capacity obtained by ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 901.45 µmol Trolox/100 g and 1607.87 µmol Trolox/100 g, respectively.
34443442	3	89	theme	different	618:626	arg1	temperatures					628:639	different temperatures	618:639	different temperatures	618:639	The experimental values of the moisture ratio during jackfruit seed drying at different temperatures were obtained using Page's equation to establish the drying time for the required moisture between 5 and 7% in the flour.
34443442	12	90	contain	has	2049:2051	arg2	activity					2106:2113	antioxidant and prebiotic activity	2080:2113	antioxidant and prebiotic activity	2080:2113	These results demonstrate that jackfruit seed flour has good nutritional value and antioxidant and prebiotic activity, as well as potential protective effects and functional properties, making it an attractive food or ingredient in developing innovative functional products.
34443442	12	90	contain	has	2049:2051	arg2	effects					2148:2154	potential protective effects	2127:2154	potential protective effects	2127:2154	These results demonstrate that jackfruit seed flour has good nutritional value and antioxidant and prebiotic activity, as well as potential protective effects and functional properties, making it an attractive food or ingredient in developing innovative functional products.
34443442	12	90	contain	has	2049:2051	arg1	flour					2043:2047	jackfruit seed flour	2028:2047	jackfruit seed flour	2028:2047	These results demonstrate that jackfruit seed flour has good nutritional value and antioxidant and prebiotic activity, as well as potential protective effects and functional properties, making it an attractive food or ingredient in developing innovative functional products.
34443442	12	90	contain	has	2049:2051	arg2	value					2070:2074	good nutritional value	2053:2074	good nutritional value	2053:2074	These results demonstrate that jackfruit seed flour has good nutritional value and antioxidant and prebiotic activity, as well as potential protective effects and functional properties, making it an attractive food or ingredient in developing innovative functional products.
34443442	12	91	theme	jackfruit	2028:2036	arg1	flour					2043:2047	jackfruit seed flour	2028:2047	jackfruit seed flour	2028:2047	These results demonstrate that jackfruit seed flour has good nutritional value and antioxidant and prebiotic activity, as well as potential protective effects and functional properties, making it an attractive food or ingredient in developing innovative functional products.
34443442	2	92	theme	drying	335:340	arg1	processes					342:350	The drying processes	331:350	The drying processes of jackfruit seeds	331:369	The drying processes of jackfruit seeds were performed at 50, 60 and 70 °C in order to choose the optimal temperature for obtaining the flour based on drying time, polyphenol content and antioxidant capacity.
34443442	1	93	theme	flour	324:328	arg1	properties					223:232	physicochemical properties	207:232	physicochemical properties	207:232	The general aim of this study was to evaluate physicochemical properties, prebiotic activity and anticancer potential of jackfruit (Artocarpus heterophyllus) seed flour.
34443442	1	93	theme	flour	324:328	arg1	activity					245:252	prebiotic activity	235:252	prebiotic activity	235:252	The general aim of this study was to evaluate physicochemical properties, prebiotic activity and anticancer potential of jackfruit (Artocarpus heterophyllus) seed flour.
34443442	1	93	theme	flour	324:328	arg1	potential					269:277	anticancer potential	258:277	anticancer potential	258:277	The general aim of this study was to evaluate physicochemical properties, prebiotic activity and anticancer potential of jackfruit (Artocarpus heterophyllus) seed flour.
34443442	9	94	theme	acid/g	1456:1461	arg1	content					1426:1432	The total polyphenol content	1405:1432	The total polyphenol content	1405:1432	The total polyphenol content was 2.42 mg of gallic acid/g of the sample; furthermore, the antioxidant capacity obtained by ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 901.45 µmol Trolox/100 g and 1607.87 µmol Trolox/100 g, respectively.
34443442	9	94	theme	acid/g	1456:1461	arg1	mg					1443:1444	2.42 mg	1438:1444	2.42 mg of gallic acid/g of the sample	1438:1475	The total polyphenol content was 2.42 mg of gallic acid/g of the sample; furthermore, the antioxidant capacity obtained by ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 901.45 µmol Trolox/100 g and 1607.87 µmol Trolox/100 g, respectively.
34443442	6	95	theme	dietary	1060:1066	arg1	g					1084:1084	31 g/100 g	1075:1084	31 g/100 g	1075:1084	The seed flour contains carbohydrates (73.87 g/100 g), dietary fiber (31 g/100 g), protein (14 g/100 g) and lipids (1 g/100 g).
34443442	6	95	theme	dietary	1060:1066	arg1	fiber					1068:1072	dietary fiber	1060:1072	dietary fiber (31 g/100 g)	1060:1085	The seed flour contains carbohydrates (73.87 g/100 g), dietary fiber (31 g/100 g), protein (14 g/100 g) and lipids (1 g/100 g).
34443442	12	96	theme	functional	2251:2260	arg1	products					2262:2269	innovative functional products	2240:2269	innovative functional products	2240:2269	These results demonstrate that jackfruit seed flour has good nutritional value and antioxidant and prebiotic activity, as well as potential protective effects and functional properties, making it an attractive food or ingredient in developing innovative functional products.
34443442	10	97	theme	good	1713:1716	arg1	water					1749:1753	water	1749:1753	water	1749:1753	The obtained flour exhibited good functional properties, such as water and oil absorption capacity, swelling power and emulsifier capacity.
34443442	10	97	theme	good	1713:1716	arg1	capacity					1774:1781	oil absorption capacity	1759:1781	oil absorption capacity	1759:1781	The obtained flour exhibited good functional properties, such as water and oil absorption capacity, swelling power and emulsifier capacity.
34443442	10	97	theme	good	1713:1716	arg1	properties					1729:1738	good functional properties	1713:1738	good functional properties	1713:1738	The obtained flour exhibited good functional properties, such as water and oil absorption capacity, swelling power and emulsifier capacity.
34443442	2	98	theme	seeds	365:369	arg1	processes					342:350	The drying processes	331:350	The drying processes of jackfruit seeds	331:369	The drying processes of jackfruit seeds were performed at 50, 60 and 70 °C in order to choose the optimal temperature for obtaining the flour based on drying time, polyphenol content and antioxidant capacity.
34443442	1	99	theme	prebiotic	235:243	arg1	activity					245:252	prebiotic activity	235:252	prebiotic activity	235:252	The general aim of this study was to evaluate physicochemical properties, prebiotic activity and anticancer potential of jackfruit (Artocarpus heterophyllus) seed flour.
32011888	7	0	theme	surface	1450:1456	arg1	functionalization					1458:1474	its surface functionalization	1446:1474	its surface functionalization with the taurocholic acid ligand	1446:1507	The studies revealed that NC-T could effectively improve the intestinal uptake and permeability, owing to its surface functionalization with the taurocholic acid ligand.
32011888	10	1	theme	therapeutic	1865:1875	arg1	peptides					1877:1884	therapeutic peptides	1865:1884	therapeutic peptides	1865:1884	As a result, the orally targeted nanocomplex might be a promising candidate for improving the oral transport of therapeutic peptides.
32011888	3	2	theme	barriers	669:676	arg1	existence					647:655	the existence	643:655	the existence of multiple barriers in the gastrointestinal (GI) tract	643:711	Oral delivery is a popular modality of administration for patients because of its convenience to self-administration and high patient compliance, while orally administered sCT remains a great challenge currently due to the existence of multiple barriers in the gastrointestinal (GI) tract.
32011888	4	3	theme	permeation	853:862	arg1	route					864:868	the paracellular permeation route	836:868	the paracellular permeation route	836:868	Here, we introduced an orally targeted delivery system to increase the transport of sCT across the intestine through both the paracellular permeation route and the bile acid pathway.
32011888	8	4	theme	oral	1606:1609	arg1	bioavailability					1611:1625	a relative oral bioavailability	1595:1625	a relative oral bioavailability of 10.9%	1595:1634	In the rat model, orally administered NC-T showed an obvious hypocalcemia effect and a relative oral bioavailability of 10.9%.
32011888	6	5	theme	high	1221:1224	arg1	reproducibility					1241:1255	high batch-to-batch reproducibility	1221:1255	high batch-to-batch reproducibility	1221:1255	The optimized NC-T exhibited well-controlled properties with a uniform and sub-60 nm hydrodynamic diameter, high batch-to-batch reproducibility, good physical or chemical stability, as well as sustained drug release behaviors.
32011888	2	6	theme	therapeutic	272:282	arg1	usefulness					284:293	the therapeutic usefulness	268:293	the therapeutic usefulness of sCT	268:300	However, the therapeutic usefulness of sCT is hindered by the frequent injection required, owing to its short plasma half-life and therapeutic need for a high dose.
32011888	7	7	with	functionalization	1458:1474	arg1	ligand					1502:1507	the taurocholic acid ligand	1481:1507	the taurocholic acid ligand	1481:1507	The studies revealed that NC-T could effectively improve the intestinal uptake and permeability, owing to its surface functionalization with the taurocholic acid ligand.
32011888	1	8	theme	peptide	172:178	arg1	hormone					180:186	a potent calcium-regulating peptide hormone	144:186	a potent calcium-regulating peptide hormone	144:186	Salmon calcitonin (sCT) is a potent calcium-regulating peptide hormone and widely applied for the treatment of some bone diseases clinically.
32011888	1	8	theme	peptide	172:178	arg1	calcitonin					124:133	Salmon calcitonin	117:133	Salmon calcitonin (sCT)	117:139	Salmon calcitonin (sCT) is a potent calcium-regulating peptide hormone and widely applied for the treatment of some bone diseases clinically.
32011888	9	9	theme	in	1640:1641	arg1	assay					1648:1652	An in vivo assay	1637:1652	An in vivo assay	1637:1652	An in vivo assay also demonstrated that NC-T induced no observable side effect after long-term oral administration.
32011888	6	10	theme	nm	1195:1196	arg1	diameter					1211:1218	sub-60 nm hydrodynamic diameter	1188:1218	sub-60 nm hydrodynamic diameter	1188:1218	The optimized NC-T exhibited well-controlled properties with a uniform and sub-60 nm hydrodynamic diameter, high batch-to-batch reproducibility, good physical or chemical stability, as well as sustained drug release behaviors.
32011888	3	11	theme	high	545:548	arg1	compliance					558:567	high patient compliance	545:567	high patient compliance	545:567	Oral delivery is a popular modality of administration for patients because of its convenience to self-administration and high patient compliance, while orally administered sCT remains a great challenge currently due to the existence of multiple barriers in the gastrointestinal (GI) tract.
32011888	6	12	theme	sub-60	1188:1193	arg1	nm					1195:1196	nm	1195:1196	nm	1195:1196	The optimized NC-T exhibited well-controlled properties with a uniform and sub-60 nm hydrodynamic diameter, high batch-to-batch reproducibility, good physical or chemical stability, as well as sustained drug release behaviors.
32011888	3	13	theme	administered	583:594	arg1	sCT					596:598	orally administered sCT	576:598	orally administered sCT	576:598	Oral delivery is a popular modality of administration for patients because of its convenience to self-administration and high patient compliance, while orally administered sCT remains a great challenge currently due to the existence of multiple barriers in the gastrointestinal (GI) tract.
32011888	5	14	theme	ternary	994:1000	arg1	NC-T					1017:1020	NC-T	1017:1020	NC-T	1017:1020	In this system, sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T) were produced by a flash nanocomplexation (FNC) process in a kinetically controlled mode.
32011888	5	14	theme	ternary	994:1000	arg1	nanocomplexes					1002:1014	sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes	913:1014	sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T)	913:1021	In this system, sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T) were produced by a flash nanocomplexation (FNC) process in a kinetically controlled mode.
32011888	4	15	theme	bile	878:881	arg1	pathway					888:894	the bile acid pathway	874:894	the bile acid pathway	874:894	Here, we introduced an orally targeted delivery system to increase the transport of sCT across the intestine through both the paracellular permeation route and the bile acid pathway.
32011888	6	16	theme	good	1258:1261	arg1	stability					1284:1292	good physical or chemical stability	1258:1292	good physical or chemical stability	1258:1292	The optimized NC-T exhibited well-controlled properties with a uniform and sub-60 nm hydrodynamic diameter, high batch-to-batch reproducibility, good physical or chemical stability, as well as sustained drug release behaviors.
32011888	3	17	theme	great	610:614	arg1	challenge					616:624	a great challenge	608:624	a great challenge currently due to the existence of multiple barriers in the gastrointestinal (GI) tract	608:711	Oral delivery is a popular modality of administration for patients because of its convenience to self-administration and high patient compliance, while orally administered sCT remains a great challenge currently due to the existence of multiple barriers in the gastrointestinal (GI) tract.
32011888	9	18	theme	side	1704:1707	arg1	effect					1709:1714	no observable side effect	1690:1714	no observable side effect	1690:1714	An in vivo assay also demonstrated that NC-T induced no observable side effect after long-term oral administration.
32011888	6	19	theme	well-controlled	1142:1156	arg1	properties					1158:1167	well-controlled properties	1142:1167	well-controlled properties	1142:1167	The optimized NC-T exhibited well-controlled properties with a uniform and sub-60 nm hydrodynamic diameter, high batch-to-batch reproducibility, good physical or chemical stability, as well as sustained drug release behaviors.
32011888	6	20	dep	uniform	1176:1182	arg1	behaviors					1329:1337	behaviors	1329:1337	behaviors	1329:1337	The optimized NC-T exhibited well-controlled properties with a uniform and sub-60 nm hydrodynamic diameter, high batch-to-batch reproducibility, good physical or chemical stability, as well as sustained drug release behaviors.
32011888	5	21	theme	FNC	1066:1068	arg1	process					1071:1077	a flash nanocomplexation (FNC) process	1040:1077	a flash nanocomplexation (FNC) process in a kinetically controlled mode	1040:1110	In this system, sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T) were produced by a flash nanocomplexation (FNC) process in a kinetically controlled mode.
32011888	0	22	theme	Salmon	77:82	arg1	Calcitonin					84:93	Salmon Calcitonin	77:93	Salmon Calcitonin	77:93	Flash Fabrication of Orally Targeted Nanocomplexes for Improved Transport of Salmon Calcitonin across the Intestine.
32011888	1	23	theme	potent	146:151	arg1	hormone					180:186	a potent calcium-regulating peptide hormone	144:186	a potent calcium-regulating peptide hormone	144:186	Salmon calcitonin (sCT) is a potent calcium-regulating peptide hormone and widely applied for the treatment of some bone diseases clinically.
32011888	1	23	theme	potent	146:151	arg1	calcitonin					124:133	Salmon calcitonin	117:133	Salmon calcitonin (sCT)	117:139	Salmon calcitonin (sCT) is a potent calcium-regulating peptide hormone and widely applied for the treatment of some bone diseases clinically.
32011888	9	24	theme	oral	1732:1735	arg1	administration					1737:1750	long-term oral administration	1722:1750	long-term oral administration	1722:1750	An in vivo assay also demonstrated that NC-T induced no observable side effect after long-term oral administration.
32011888	6	25	theme	drug	1316:1319	arg1	release					1321:1327	sustained drug release	1306:1327	sustained drug release	1306:1327	The optimized NC-T exhibited well-controlled properties with a uniform and sub-60 nm hydrodynamic diameter, high batch-to-batch reproducibility, good physical or chemical stability, as well as sustained drug release behaviors.
32011888	6	26	theme	optimized	1117:1125	arg1	NC-T					1127:1130	The optimized NC-T	1113:1130	The optimized NC-T	1113:1130	The optimized NC-T exhibited well-controlled properties with a uniform and sub-60 nm hydrodynamic diameter, high batch-to-batch reproducibility, good physical or chemical stability, as well as sustained drug release behaviors.
32011888	5	27	theme	chitosan-taurocholic	930:949	arg1	DS					990:991	DS	990:991	DS	990:991	In this system, sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T) were produced by a flash nanocomplexation (FNC) process in a kinetically controlled mode.
32011888	5	27	theme	chitosan-taurocholic	930:949	arg1	sulfate					981:987	sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate	913:987	sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T)	913:1021	In this system, sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T) were produced by a flash nanocomplexation (FNC) process in a kinetically controlled mode.
32011888	4	28	theme	targeted	744:751	arg1	system					762:767	an orally targeted delivery system	734:767	an orally targeted delivery system to increase the transport of sCT across the intestine through both the paracellular permeation route and the bile acid pathway	734:894	Here, we introduced an orally targeted delivery system to increase the transport of sCT across the intestine through both the paracellular permeation route and the bile acid pathway.
32011888	5	29	theme	nanocomplexation	1048:1063	arg1	process					1071:1077	a flash nanocomplexation (FNC) process	1040:1077	a flash nanocomplexation (FNC) process in a kinetically controlled mode	1040:1110	In this system, sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T) were produced by a flash nanocomplexation (FNC) process in a kinetically controlled mode.
32011888	0	30	theme	Nanocomplexes	37:49	arg1	Fabrication					6:16	Flash Fabrication	0:16	Flash Fabrication of Orally Targeted Nanocomplexes for Improved Transport of Salmon Calcitonin across the Intestine.	0:115	Flash Fabrication of Orally Targeted Nanocomplexes for Improved Transport of Salmon Calcitonin across the Intestine.
32011888	5	31	theme	conjugate	956:964	arg1	DS					990:991	DS	990:991	DS	990:991	In this system, sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T) were produced by a flash nanocomplexation (FNC) process in a kinetically controlled mode.
32011888	5	31	theme	conjugate	956:964	arg1	sulfate					981:987	sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate	913:987	sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T)	913:1021	In this system, sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T) were produced by a flash nanocomplexation (FNC) process in a kinetically controlled mode.
32011888	2	32	theme	short	363:367	arg1	half-life					376:384	its short plasma half-life	359:384	its short plasma half-life	359:384	However, the therapeutic usefulness of sCT is hindered by the frequent injection required, owing to its short plasma half-life and therapeutic need for a high dose.
32011888	0	33	theme	Flash	0:4	arg1	Fabrication					6:16	Flash Fabrication	0:16	Flash Fabrication of Orally Targeted Nanocomplexes for Improved Transport of Salmon Calcitonin across the Intestine.	0:115	Flash Fabrication of Orally Targeted Nanocomplexes for Improved Transport of Salmon Calcitonin across the Intestine.
32011888	2	34	theme	high	413:416	arg1	dose					418:421	a high dose	411:421	a high dose	411:421	However, the therapeutic usefulness of sCT is hindered by the frequent injection required, owing to its short plasma half-life and therapeutic need for a high dose.
32011888	5	35	theme	sCT-based	913:921	arg1	DS					990:991	DS	990:991	DS	990:991	In this system, sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T) were produced by a flash nanocomplexation (FNC) process in a kinetically controlled mode.
32011888	5	35	theme	sCT-based	913:921	arg1	sulfate					981:987	sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate	913:987	sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T)	913:1021	In this system, sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T) were produced by a flash nanocomplexation (FNC) process in a kinetically controlled mode.
32011888	9	36	dep	in	1640:1641	arg1	vivo					1643:1646	vivo	1643:1646	vivo	1643:1646	An in vivo assay also demonstrated that NC-T induced no observable side effect after long-term oral administration.
32011888	10	37	theme	promising	1809:1817	arg1	candidate					1819:1827	a promising candidate	1807:1827	a promising candidate for improving the oral transport of therapeutic peptides	1807:1884	As a result, the orally targeted nanocomplex might be a promising candidate for improving the oral transport of therapeutic peptides.
32011888	10	37	theme	promising	1809:1817	arg1	nanocomplex					1786:1796	the orally targeted nanocomplex	1766:1796	the orally targeted nanocomplex	1766:1796	As a result, the orally targeted nanocomplex might be a promising candidate for improving the oral transport of therapeutic peptides.
32011888	3	38	theme	Oral	424:427	arg1	delivery					429:436	Oral delivery	424:436	Oral delivery	424:436	Oral delivery is a popular modality of administration for patients because of its convenience to self-administration and high patient compliance, while orally administered sCT remains a great challenge currently due to the existence of multiple barriers in the gastrointestinal (GI) tract.
32011888	3	38	theme	Oral	424:427	arg1	modality					451:458	a popular modality	441:458	a popular modality of administration for patients	441:489	Oral delivery is a popular modality of administration for patients because of its convenience to self-administration and high patient compliance, while orally administered sCT remains a great challenge currently due to the existence of multiple barriers in the gastrointestinal (GI) tract.
32011888	0	39	theme	Targeted	28:35	arg1	Nanocomplexes					37:49	Orally Targeted Nanocomplexes	21:49	Orally Targeted Nanocomplexes	21:49	Flash Fabrication of Orally Targeted Nanocomplexes for Improved Transport of Salmon Calcitonin across the Intestine.
32011888	7	40	theme	acid	1497:1500	arg1	ligand					1502:1507	the taurocholic acid ligand	1481:1507	the taurocholic acid ligand	1481:1507	The studies revealed that NC-T could effectively improve the intestinal uptake and permeability, owing to its surface functionalization with the taurocholic acid ligand.
32011888	4	41	theme	sCT	798:800	arg1	transport					785:793	the transport	781:793	the transport of sCT across the intestine	781:821	Here, we introduced an orally targeted delivery system to increase the transport of sCT across the intestine through both the paracellular permeation route and the bile acid pathway.
32011888	5	42	theme	controlled	1096:1105	arg1	mode					1107:1110	a kinetically controlled mode	1082:1110	a kinetically controlled mode	1082:1110	In this system, sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T) were produced by a flash nanocomplexation (FNC) process in a kinetically controlled mode.
32011888	3	43	theme	multiple	660:667	arg1	barriers					669:676	multiple barriers	660:676	multiple barriers	660:676	Oral delivery is a popular modality of administration for patients because of its convenience to self-administration and high patient compliance, while orally administered sCT remains a great challenge currently due to the existence of multiple barriers in the gastrointestinal (GI) tract.
32011888	3	44	theme	popular	443:449	arg1	delivery					429:436	Oral delivery	424:436	Oral delivery	424:436	Oral delivery is a popular modality of administration for patients because of its convenience to self-administration and high patient compliance, while orally administered sCT remains a great challenge currently due to the existence of multiple barriers in the gastrointestinal (GI) tract.
32011888	3	44	theme	popular	443:449	arg1	modality					451:458	a popular modality	441:458	a popular modality of administration for patients	441:489	Oral delivery is a popular modality of administration for patients because of its convenience to self-administration and high patient compliance, while orally administered sCT remains a great challenge currently due to the existence of multiple barriers in the gastrointestinal (GI) tract.
32011888	5	45	theme	GC-T	967:970	arg1	DS					990:991	DS	990:991	DS	990:991	In this system, sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T) were produced by a flash nanocomplexation (FNC) process in a kinetically controlled mode.
32011888	5	45	theme	GC-T	967:970	arg1	sulfate					981:987	sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate	913:987	sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T)	913:1021	In this system, sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T) were produced by a flash nanocomplexation (FNC) process in a kinetically controlled mode.
32011888	5	46	theme	/dextran	972:979	arg1	DS					990:991	DS	990:991	DS	990:991	In this system, sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T) were produced by a flash nanocomplexation (FNC) process in a kinetically controlled mode.
32011888	5	46	theme	/dextran	972:979	arg1	sulfate					981:987	sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate	913:987	sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T)	913:1021	In this system, sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T) were produced by a flash nanocomplexation (FNC) process in a kinetically controlled mode.
32011888	8	47	theme	hypocalcemia	1571:1582	arg1	effect					1584:1589	an obvious hypocalcemia effect	1560:1589	an obvious hypocalcemia effect	1560:1589	In the rat model, orally administered NC-T showed an obvious hypocalcemia effect and a relative oral bioavailability of 10.9%.
32011888	3	48	theme	gastrointestinal	685:700	arg1	tract					707:711	the gastrointestinal (GI) tract	681:711	the gastrointestinal (GI) tract	681:711	Oral delivery is a popular modality of administration for patients because of its convenience to self-administration and high patient compliance, while orally administered sCT remains a great challenge currently due to the existence of multiple barriers in the gastrointestinal (GI) tract.
32011888	1	49	theme	diseases	238:245	arg1	treatment					215:223	the treatment	211:223	the treatment of some bone diseases	211:245	Salmon calcitonin (sCT) is a potent calcium-regulating peptide hormone and widely applied for the treatment of some bone diseases clinically.
32011888	6	50	theme	batch-to-batch	1226:1239	arg1	reproducibility					1241:1255	high batch-to-batch reproducibility	1221:1255	high batch-to-batch reproducibility	1221:1255	The optimized NC-T exhibited well-controlled properties with a uniform and sub-60 nm hydrodynamic diameter, high batch-to-batch reproducibility, good physical or chemical stability, as well as sustained drug release behaviors.
32011888	4	51	theme	paracellular	840:851	arg1	route					864:868	the paracellular permeation route	836:868	the paracellular permeation route	836:868	Here, we introduced an orally targeted delivery system to increase the transport of sCT across the intestine through both the paracellular permeation route and the bile acid pathway.
32011888	7	52	theme	intestinal	1401:1410	arg1	uptake					1412:1417	the intestinal uptake	1397:1417	the intestinal uptake	1397:1417	The studies revealed that NC-T could effectively improve the intestinal uptake and permeability, owing to its surface functionalization with the taurocholic acid ligand.
32011888	8	53	theme	relative	1597:1604	arg1	bioavailability					1611:1625	a relative oral bioavailability	1595:1625	a relative oral bioavailability of 10.9%	1595:1634	In the rat model, orally administered NC-T showed an obvious hypocalcemia effect and a relative oral bioavailability of 10.9%.
32011888	1	54	theme	calcium-regulating	153:170	arg1	hormone					180:186	a potent calcium-regulating peptide hormone	144:186	a potent calcium-regulating peptide hormone	144:186	Salmon calcitonin (sCT) is a potent calcium-regulating peptide hormone and widely applied for the treatment of some bone diseases clinically.
32011888	1	54	theme	calcium-regulating	153:170	arg1	calcitonin					124:133	Salmon calcitonin	117:133	Salmon calcitonin (sCT)	117:139	Salmon calcitonin (sCT) is a potent calcium-regulating peptide hormone and widely applied for the treatment of some bone diseases clinically.
32011888	3	55	theme	GI	703:704	arg1	tract					707:711	the gastrointestinal (GI) tract	681:711	the gastrointestinal (GI) tract	681:711	Oral delivery is a popular modality of administration for patients because of its convenience to self-administration and high patient compliance, while orally administered sCT remains a great challenge currently due to the existence of multiple barriers in the gastrointestinal (GI) tract.
32011888	6	56	theme	hydrodynamic	1198:1209	arg1	diameter					1211:1218	sub-60 nm hydrodynamic diameter	1188:1218	sub-60 nm hydrodynamic diameter	1188:1218	The optimized NC-T exhibited well-controlled properties with a uniform and sub-60 nm hydrodynamic diameter, high batch-to-batch reproducibility, good physical or chemical stability, as well as sustained drug release behaviors.
32011888	3	57	theme	patient	550:556	arg1	compliance					558:567	high patient compliance	545:567	high patient compliance	545:567	Oral delivery is a popular modality of administration for patients because of its convenience to self-administration and high patient compliance, while orally administered sCT remains a great challenge currently due to the existence of multiple barriers in the gastrointestinal (GI) tract.
32011888	2	58	theme	sCT	298:300	arg1	usefulness					284:293	the therapeutic usefulness	268:293	the therapeutic usefulness of sCT	268:300	However, the therapeutic usefulness of sCT is hindered by the frequent injection required, owing to its short plasma half-life and therapeutic need for a high dose.
32011888	4	59	theme	acid	883:886	arg1	pathway					888:894	the bile acid pathway	874:894	the bile acid pathway	874:894	Here, we introduced an orally targeted delivery system to increase the transport of sCT across the intestine through both the paracellular permeation route and the bile acid pathway.
32011888	2	60	theme	frequent	321:328	arg1	injection					330:338	the frequent injection	317:338	the frequent injection required	317:347	However, the therapeutic usefulness of sCT is hindered by the frequent injection required, owing to its short plasma half-life and therapeutic need for a high dose.
32011888	6	61	theme	chemical	1275:1282	arg1	stability					1284:1292	good physical or chemical stability	1258:1292	good physical or chemical stability	1258:1292	The optimized NC-T exhibited well-controlled properties with a uniform and sub-60 nm hydrodynamic diameter, high batch-to-batch reproducibility, good physical or chemical stability, as well as sustained drug release behaviors.
32011888	1	62	theme	Salmon	117:122	arg1	hormone					180:186	a potent calcium-regulating peptide hormone	144:186	a potent calcium-regulating peptide hormone	144:186	Salmon calcitonin (sCT) is a potent calcium-regulating peptide hormone and widely applied for the treatment of some bone diseases clinically.
32011888	1	62	theme	Salmon	117:122	arg1	sCT					136:138	sCT	136:138	sCT	136:138	Salmon calcitonin (sCT) is a potent calcium-regulating peptide hormone and widely applied for the treatment of some bone diseases clinically.
32011888	1	62	theme	Salmon	117:122	arg1	calcitonin					124:133	Salmon calcitonin	117:133	Salmon calcitonin (sCT)	117:139	Salmon calcitonin (sCT) is a potent calcium-regulating peptide hormone and widely applied for the treatment of some bone diseases clinically.
32011888	6	63	theme	physical	1263:1270	arg1	stability					1284:1292	good physical or chemical stability	1258:1292	good physical or chemical stability	1258:1292	The optimized NC-T exhibited well-controlled properties with a uniform and sub-60 nm hydrodynamic diameter, high batch-to-batch reproducibility, good physical or chemical stability, as well as sustained drug release behaviors.
32011888	9	64	theme	observable	1693:1702	arg1	effect					1709:1714	no observable side effect	1690:1714	no observable side effect	1690:1714	An in vivo assay also demonstrated that NC-T induced no observable side effect after long-term oral administration.
32011888	10	65	theme	targeted	1777:1784	arg1	candidate					1819:1827	a promising candidate	1807:1827	a promising candidate for improving the oral transport of therapeutic peptides	1807:1884	As a result, the orally targeted nanocomplex might be a promising candidate for improving the oral transport of therapeutic peptides.
32011888	10	65	theme	targeted	1777:1784	arg1	nanocomplex					1786:1796	the orally targeted nanocomplex	1766:1796	the orally targeted nanocomplex	1766:1796	As a result, the orally targeted nanocomplex might be a promising candidate for improving the oral transport of therapeutic peptides.
32011888	0	66	theme	Calcitonin	84:93	arg1	Transport					64:72	Improved Transport	55:72	Improved Transport of Salmon Calcitonin across the Intestine	55:114	Flash Fabrication of Orally Targeted Nanocomplexes for Improved Transport of Salmon Calcitonin across the Intestine.
32011888	9	67	theme	long-term	1722:1730	arg1	administration					1737:1750	long-term oral administration	1722:1750	long-term oral administration	1722:1750	An in vivo assay also demonstrated that NC-T induced no observable side effect after long-term oral administration.
32011888	5	68	theme	glycol	923:928	arg1	DS					990:991	DS	990:991	DS	990:991	In this system, sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T) were produced by a flash nanocomplexation (FNC) process in a kinetically controlled mode.
32011888	5	68	theme	glycol	923:928	arg1	sulfate					981:987	sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate	913:987	sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T)	913:1021	In this system, sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T) were produced by a flash nanocomplexation (FNC) process in a kinetically controlled mode.
32011888	6	69	theme	sustained	1306:1314	arg1	release					1321:1327	sustained drug release	1306:1327	sustained drug release	1306:1327	The optimized NC-T exhibited well-controlled properties with a uniform and sub-60 nm hydrodynamic diameter, high batch-to-batch reproducibility, good physical or chemical stability, as well as sustained drug release behaviors.
32011888	5	70	theme	flash	1042:1046	arg1	process					1071:1077	a flash nanocomplexation (FNC) process	1040:1077	a flash nanocomplexation (FNC) process in a kinetically controlled mode	1040:1110	In this system, sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T) were produced by a flash nanocomplexation (FNC) process in a kinetically controlled mode.
32011888	5	71	theme	acid	951:954	arg1	DS					990:991	DS	990:991	DS	990:991	In this system, sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T) were produced by a flash nanocomplexation (FNC) process in a kinetically controlled mode.
32011888	5	71	theme	acid	951:954	arg1	sulfate					981:987	sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate	913:987	sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T)	913:1021	In this system, sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T) were produced by a flash nanocomplexation (FNC) process in a kinetically controlled mode.
32011888	2	72	theme	therapeutic	390:400	arg1	need					402:405	therapeutic need	390:405	therapeutic need for a high dose	390:421	However, the therapeutic usefulness of sCT is hindered by the frequent injection required, owing to its short plasma half-life and therapeutic need for a high dose.
32011888	2	73	theme	plasma	369:374	arg1	half-life					376:384	its short plasma half-life	359:384	its short plasma half-life	359:384	However, the therapeutic usefulness of sCT is hindered by the frequent injection required, owing to its short plasma half-life and therapeutic need for a high dose.
32011888	8	74	theme	rat	1517:1519	arg1	model					1521:1525	the rat model	1513:1525	the rat model	1513:1525	In the rat model, orally administered NC-T showed an obvious hypocalcemia effect and a relative oral bioavailability of 10.9%.
32011888	3	75	theme	due	636:638	arg1	challenge					616:624	a great challenge	608:624	a great challenge currently due to the existence of multiple barriers in the gastrointestinal (GI) tract	608:711	Oral delivery is a popular modality of administration for patients because of its convenience to self-administration and high patient compliance, while orally administered sCT remains a great challenge currently due to the existence of multiple barriers in the gastrointestinal (GI) tract.
32011888	5	76	from	process	1071:1077	arg1	mode					1107:1110	a kinetically controlled mode	1082:1110	a kinetically controlled mode	1082:1110	In this system, sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T) were produced by a flash nanocomplexation (FNC) process in a kinetically controlled mode.
32011888	5	77	theme	sulfate	981:987	arg1	NC-T					1017:1020	NC-T	1017:1020	NC-T	1017:1020	In this system, sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T) were produced by a flash nanocomplexation (FNC) process in a kinetically controlled mode.
32011888	5	77	theme	sulfate	981:987	arg1	nanocomplexes					1002:1014	sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes	913:1014	sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T)	913:1021	In this system, sCT-based glycol chitosan-taurocholic acid conjugate (GC-T)/dextran sulfate (DS) ternary nanocomplexes (NC-T) were produced by a flash nanocomplexation (FNC) process in a kinetically controlled mode.
32011888	8	78	theme	administered	1535:1546	arg1	NC-T					1548:1551	orally administered NC-T	1528:1551	orally administered NC-T	1528:1551	In the rat model, orally administered NC-T showed an obvious hypocalcemia effect and a relative oral bioavailability of 10.9%.
32011888	0	79	theme	Improved	55:62	arg1	Transport					64:72	Improved Transport	55:72	Improved Transport of Salmon Calcitonin across the Intestine	55:114	Flash Fabrication of Orally Targeted Nanocomplexes for Improved Transport of Salmon Calcitonin across the Intestine.
32011888	10	80	theme	oral	1847:1850	arg1	transport					1852:1860	the oral transport	1843:1860	the oral transport of therapeutic peptides	1843:1884	As a result, the orally targeted nanocomplex might be a promising candidate for improving the oral transport of therapeutic peptides.
32011888	3	81	theme	administration	463:476	arg1	delivery					429:436	Oral delivery	424:436	Oral delivery	424:436	Oral delivery is a popular modality of administration for patients because of its convenience to self-administration and high patient compliance, while orally administered sCT remains a great challenge currently due to the existence of multiple barriers in the gastrointestinal (GI) tract.
32011888	3	81	theme	administration	463:476	arg1	modality					451:458	a popular modality	441:458	a popular modality of administration for patients	441:489	Oral delivery is a popular modality of administration for patients because of its convenience to self-administration and high patient compliance, while orally administered sCT remains a great challenge currently due to the existence of multiple barriers in the gastrointestinal (GI) tract.
32011888	7	82	theme	taurocholic	1485:1495	arg1	acid					1497:1500	the taurocholic acid	1481:1500	the taurocholic acid ligand	1481:1507	The studies revealed that NC-T could effectively improve the intestinal uptake and permeability, owing to its surface functionalization with the taurocholic acid ligand.
32011888	8	83	theme	obvious	1563:1569	arg1	effect					1584:1589	an obvious hypocalcemia effect	1560:1589	an obvious hypocalcemia effect	1560:1589	In the rat model, orally administered NC-T showed an obvious hypocalcemia effect and a relative oral bioavailability of 10.9%.
32011888	4	84	theme	delivery	753:760	arg1	system					762:767	an orally targeted delivery system	734:767	an orally targeted delivery system to increase the transport of sCT across the intestine through both the paracellular permeation route and the bile acid pathway	734:894	Here, we introduced an orally targeted delivery system to increase the transport of sCT across the intestine through both the paracellular permeation route and the bile acid pathway.
32011888	8	85	theme	%	1634:1634	arg1	bioavailability					1611:1625	a relative oral bioavailability	1595:1625	a relative oral bioavailability of 10.9%	1595:1634	In the rat model, orally administered NC-T showed an obvious hypocalcemia effect and a relative oral bioavailability of 10.9%.
32011888	8	85	theme	%	1634:1634	arg1	effect					1584:1589	an obvious hypocalcemia effect	1560:1589	an obvious hypocalcemia effect	1560:1589	In the rat model, orally administered NC-T showed an obvious hypocalcemia effect and a relative oral bioavailability of 10.9%.
32011888	3	86	from	existence	647:655	arg1	tract					707:711	the gastrointestinal (GI) tract	681:711	the gastrointestinal (GI) tract	681:711	Oral delivery is a popular modality of administration for patients because of its convenience to self-administration and high patient compliance, while orally administered sCT remains a great challenge currently due to the existence of multiple barriers in the gastrointestinal (GI) tract.
32011888	10	87	theme	peptides	1877:1884	arg1	transport					1852:1860	the oral transport	1843:1860	the oral transport of therapeutic peptides	1843:1884	As a result, the orally targeted nanocomplex might be a promising candidate for improving the oral transport of therapeutic peptides.
32011888	1	88	theme	bone	233:236	arg1	diseases					238:245	some bone diseases	228:245	some bone diseases	228:245	Salmon calcitonin (sCT) is a potent calcium-regulating peptide hormone and widely applied for the treatment of some bone diseases clinically.
34441532	7	0	theme	II	686:687	arg1	polysaccharides					734:748	the dominant polysaccharides	721:748	the dominant polysaccharides	721:748	Rhamnogalacturonan II pectins and α-(1→4)-glucan were the dominant polysaccharides.
34441532	7	0	theme	II	686:687	arg1	pectins					689:695	Rhamnogalacturonan II pectins	667:695	Rhamnogalacturonan II pectins	667:695	Rhamnogalacturonan II pectins and α-(1→4)-glucan were the dominant polysaccharides.
34441532	7	0	theme	II	686:687	arg1	-glucan					708:714	α-(1→4)-glucan	701:714	α-(1→4)-glucan	701:714	Rhamnogalacturonan II pectins and α-(1→4)-glucan were the dominant polysaccharides.
34441532	10	1	theme	Inner	882:886	arg1	APS					897:899	Inner Mongolia APS	882:899	Inner Mongolia APS	882:899	Inner Mongolia APS had the largest percentage of 20-40 kDa polysaccharides.
34441532	0	2	from	Differences	10:20	arg1	membranaceus					69:80	Astragalus membranaceus	58:80	Astragalus membranaceus	58:80	Structure Differences of Water Soluble Polysaccharides in Astragalus membranaceus Induced by Origin and Their Bioactivity.
34441532	1	3	theme	multiple	173:180	arg1	bioactivities					182:194	multiple bioactivities	173:194	multiple bioactivities	173:194	Astragalus membranaceus is a functional food with multiple bioactivities.
34441532	3	4	theme	health	371:376	arg1	benefits					378:385	its health benefits	367:385	its health benefits	367:385	Polysaccharides (APS) are the leading bioactive macromolecules of A. membranaceus, which are highly related to its health benefits.
34441532	6	5	theme	monosaccharide	616:629	arg1	composition					631:641	identical monosaccharide composition	606:641	identical monosaccharide composition	606:641	The results showed APSs of four origins had identical monosaccharide composition and glycosidic linkage.
34441532	3	6	dep	A.	322:323	arg1	related					356:362	related	356:362	related	356:362	Polysaccharides (APS) are the leading bioactive macromolecules of A. membranaceus, which are highly related to its health benefits.
34441532	3	6	dep	A.	322:323	arg1	membranaceus					325:336	A. membranaceus, which are highly related to its health benefits	322:385	membranaceus	325:336	Polysaccharides (APS) are the leading bioactive macromolecules of A. membranaceus, which are highly related to its health benefits.
34441532	1	7	with	food	163:166	arg1	bioactivities					182:194	multiple bioactivities	173:194	multiple bioactivities	173:194	Astragalus membranaceus is a functional food with multiple bioactivities.
34441532	10	8	theme	largest	909:915	arg1	percentage					917:926	the largest percentage	905:926	the largest percentage of 20-40 kDa polysaccharides	905:955	Inner Mongolia APS had the largest percentage of 20-40 kDa polysaccharides.
34441532	7	9	theme	dominant	725:732	arg1	polysaccharides					734:748	the dominant polysaccharides	721:748	the dominant polysaccharides	721:748	Rhamnogalacturonan II pectins and α-(1→4)-glucan were the dominant polysaccharides.
34441532	7	9	theme	dominant	725:732	arg1	pectins					689:695	Rhamnogalacturonan II pectins	667:695	Rhamnogalacturonan II pectins	667:695	Rhamnogalacturonan II pectins and α-(1→4)-glucan were the dominant polysaccharides.
34441532	7	9	theme	dominant	725:732	arg1	-glucan					708:714	α-(1→4)-glucan	701:714	α-(1→4)-glucan	701:714	Rhamnogalacturonan II pectins and α-(1→4)-glucan were the dominant polysaccharides.
34441532	8	10	from	level	764:768	arg1	pectins					789:795	pectins	789:795	pectins	789:795	However, the level of methyl ester in pectins varied to a large extent.
34441532	4	11	theme	origin	411:416	arg1	effect					401:406	the effect	397:406	the effect of origin on the structural characteristics of APSs	397:458	However, the effect of origin on the structural characteristics of APSs remains unclear.
34441532	9	12	theme	weight	837:842	arg1	different					871:879	different	871:879	different	871:879	The molecular weight profiles of APSs were also different.
34441532	9	12	theme	weight	837:842	arg1	profiles					844:851	The molecular weight profiles	823:851	The molecular weight profiles of APSs	823:859	The molecular weight profiles of APSs were also different.
34441532	4	13	from	effect	401:406	arg1	characteristics					436:450	the structural characteristics	421:450	the structural characteristics of APSs	421:458	However, the effect of origin on the structural characteristics of APSs remains unclear.
34441532	6	14	theme	identical	606:614	arg1	composition					631:641	identical monosaccharide composition	606:641	identical monosaccharide composition	606:641	The results showed APSs of four origins had identical monosaccharide composition and glycosidic linkage.
34441532	10	15	theme	polysaccharides	941:955	arg1	percentage					917:926	the largest percentage	905:926	the largest percentage of 20-40 kDa polysaccharides	905:955	Inner Mongolia APS had the largest percentage of 20-40 kDa polysaccharides.
34441532	0	16	theme	Structure	0:8	arg1	Differences					10:20	Structure Differences	0:20	Structure Differences of Water Soluble Polysaccharides in Astragalus membranaceus	0:80	Structure Differences of Water Soluble Polysaccharides in Astragalus membranaceus Induced by Origin and Their Bioactivity.
34441532	7	17	theme	α-	701:702	arg1	polysaccharides					734:748	the dominant polysaccharides	721:748	the dominant polysaccharides	721:748	Rhamnogalacturonan II pectins and α-(1→4)-glucan were the dominant polysaccharides.
34441532	7	17	theme	α-	701:702	arg1	pectins					689:695	Rhamnogalacturonan II pectins	667:695	Rhamnogalacturonan II pectins	667:695	Rhamnogalacturonan II pectins and α-(1→4)-glucan were the dominant polysaccharides.
34441532	7	17	theme	α-	701:702	arg1	-glucan					708:714	α-(1→4)-glucan	701:714	α-(1→4)-glucan	701:714	Rhamnogalacturonan II pectins and α-(1→4)-glucan were the dominant polysaccharides.
34441532	9	18	theme	APSs	856:859	arg1	different					871:879	different	871:879	different	871:879	The molecular weight profiles of APSs were also different.
34441532	9	18	theme	APSs	856:859	arg1	profiles					844:851	The molecular weight profiles	823:851	The molecular weight profiles of APSs	823:859	The molecular weight profiles of APSs were also different.
34441532	0	19	theme	Water	25:29	arg1	Polysaccharides					39:53	Water Soluble Polysaccharides	25:53	Water Soluble Polysaccharides	25:53	Structure Differences of Water Soluble Polysaccharides in Astragalus membranaceus Induced by Origin and Their Bioactivity.
34441532	3	20	theme	bioactive	294:302	arg1	macromolecules					304:317	the leading bioactive macromolecules	282:317	the leading bioactive macromolecules of A. membranaceus, which are highly related to its health benefits	282:385	Polysaccharides (APS) are the leading bioactive macromolecules of A. membranaceus, which are highly related to its health benefits.
34441532	3	20	theme	bioactive	294:302	arg1	Polysaccharides					256:270	Polysaccharides	256:270	Polysaccharides (APS)	256:276	Polysaccharides (APS) are the leading bioactive macromolecules of A. membranaceus, which are highly related to its health benefits.
34441532	10	21	theme	kDa	937:939	arg1	polysaccharides					941:955	20-40 kDa polysaccharides	931:955	20-40 kDa polysaccharides	931:955	Inner Mongolia APS had the largest percentage of 20-40 kDa polysaccharides.
34441532	11	22	theme	important	1007:1015	arg1	parameters					1017:1026	two important parameters	1003:1026	two important parameters determining the difference of APSs from four origins	1003:1079	Molecular weight and methyl ester level were two important parameters determining the difference of APSs from four origins.
34441532	0	23	theme	Polysaccharides	39:53	arg1	Differences					10:20	Structure Differences	0:20	Structure Differences of Water Soluble Polysaccharides in Astragalus membranaceus	0:80	Structure Differences of Water Soluble Polysaccharides in Astragalus membranaceus Induced by Origin and Their Bioactivity.
34441532	11	24	theme	Molecular	958:966	arg1	weight					968:973	Molecular weight	958:973	Molecular weight	958:973	Molecular weight and methyl ester level were two important parameters determining the difference of APSs from four origins.
34441532	4	25	theme	APSs	455:458	arg1	characteristics					436:450	the structural characteristics	421:450	the structural characteristics of APSs	421:458	However, the effect of origin on the structural characteristics of APSs remains unclear.
34441532	12	26	theme	membranaceus	1155:1166	arg1	quality					1141:1147	the origin-related quality	1122:1147	the origin-related quality of A. membranaceus	1122:1166	These results were helpful to recognize the origin-related quality of A. membranaceus.
34441532	0	27	theme	Soluble	31:37	arg1	Polysaccharides					39:53	Water Soluble Polysaccharides	25:53	Water Soluble Polysaccharides	25:53	Structure Differences of Water Soluble Polysaccharides in Astragalus membranaceus Induced by Origin and Their Bioactivity.
34441532	9	28	theme	molecular	827:835	arg1	different					871:879	different	871:879	different	871:879	The molecular weight profiles of APSs were also different.
34441532	9	28	theme	molecular	827:835	arg1	profiles					844:851	The molecular weight profiles	823:851	The molecular weight profiles of APSs	823:859	The molecular weight profiles of APSs were also different.
34441532	3	29	theme	leading	286:292	arg1	macromolecules					304:317	the leading bioactive macromolecules	282:317	the leading bioactive macromolecules of A. membranaceus, which are highly related to its health benefits	282:385	Polysaccharides (APS) are the leading bioactive macromolecules of A. membranaceus, which are highly related to its health benefits.
34441532	3	29	theme	leading	286:292	arg1	Polysaccharides					256:270	Polysaccharides	256:270	Polysaccharides (APS)	256:276	Polysaccharides (APS) are the leading bioactive macromolecules of A. membranaceus, which are highly related to its health benefits.
34441532	7	30	theme	1→4	704:706	arg1	polysaccharides					734:748	the dominant polysaccharides	721:748	the dominant polysaccharides	721:748	Rhamnogalacturonan II pectins and α-(1→4)-glucan were the dominant polysaccharides.
34441532	7	30	theme	1→4	704:706	arg1	pectins					689:695	Rhamnogalacturonan II pectins	667:695	Rhamnogalacturonan II pectins	667:695	Rhamnogalacturonan II pectins and α-(1→4)-glucan were the dominant polysaccharides.
34441532	7	30	theme	1→4	704:706	arg1	-glucan					708:714	α-(1→4)-glucan	701:714	α-(1→4)-glucan	701:714	Rhamnogalacturonan II pectins and α-(1→4)-glucan were the dominant polysaccharides.
34441532	6	31	theme	origins	594:600	arg1	APSs					581:584	APSs	581:584	APSs of four origins	581:600	The results showed APSs of four origins had identical monosaccharide composition and glycosidic linkage.
34441532	0	32	theme	Astragalus	58:67	arg1	membranaceus					69:80	Astragalus membranaceus	58:80	Astragalus membranaceus	58:80	Structure Differences of Water Soluble Polysaccharides in Astragalus membranaceus Induced by Origin and Their Bioactivity.
34441532	1	33	theme	Astragalus	123:132	arg1	food					163:166	a functional food	150:166	a functional food with multiple bioactivities	150:194	Astragalus membranaceus is a functional food with multiple bioactivities.
34441532	1	33	theme	Astragalus	123:132	arg1	membranaceus					134:145	Astragalus membranaceus	123:145	Astragalus membranaceus	123:145	Astragalus membranaceus is a functional food with multiple bioactivities.
34441532	11	34	theme	methyl	979:984	arg1	level					992:996	methyl ester level	979:996	methyl ester level	979:996	Molecular weight and methyl ester level were two important parameters determining the difference of APSs from four origins.
34441532	6	35	dep	showed	574:579	arg1	had					602:604	had	602:604	showed APSs of four origins had identical monosaccharide composition and glycosidic linkage	574:664	The results showed APSs of four origins had identical monosaccharide composition and glycosidic linkage.
34441532	4	36	theme	structural	425:434	arg1	characteristics					436:450	the structural characteristics	421:450	the structural characteristics of APSs	421:458	However, the effect of origin on the structural characteristics of APSs remains unclear.
34441532	11	37	theme	ester	986:990	arg1	level					992:996	methyl ester level	979:996	methyl ester level	979:996	Molecular weight and methyl ester level were two important parameters determining the difference of APSs from four origins.
34441532	8	38	theme	large	809:813	arg1	extent					815:820	a large extent	807:820	a large extent	807:820	However, the level of methyl ester in pectins varied to a large extent.
34441532	8	39	theme	methyl	773:778	arg1	ester					780:784	methyl ester	773:784	methyl ester	773:784	However, the level of methyl ester in pectins varied to a large extent.
34441532	3	40	theme	A.	322:323	arg1	macromolecules					304:317	the leading bioactive macromolecules	282:317	the leading bioactive macromolecules of A. membranaceus, which are highly related to its health benefits	282:385	Polysaccharides (APS) are the leading bioactive macromolecules of A. membranaceus, which are highly related to its health benefits.
34441532	3	40	theme	A.	322:323	arg1	Polysaccharides					256:270	Polysaccharides	256:270	Polysaccharides (APS)	256:276	Polysaccharides (APS) are the leading bioactive macromolecules of A. membranaceus, which are highly related to its health benefits.
34441532	2	41	theme	health	224:229	arg1	benefits					231:238	differentiated health benefits	209:238	differentiated health benefits	209:238	It presents differentiated health benefits due to origins.
34441532	5	42	from	origins	517:523	arg1	polysaccharides					491:505	polysaccharides	491:505	polysaccharides from four origins	491:523	In this work, polysaccharides from four origins were isolated and identified by NMR.
34441532	11	43	theme	APSs	1058:1061	arg1	difference					1044:1053	the difference	1040:1053	the difference of APSs	1040:1061	Molecular weight and methyl ester level were two important parameters determining the difference of APSs from four origins.
34441532	10	44	contain	had	901:903	arg2	percentage					917:926	the largest percentage	905:926	the largest percentage of 20-40 kDa polysaccharides	905:955	Inner Mongolia APS had the largest percentage of 20-40 kDa polysaccharides.
34441532	10	44	contain	had	901:903	arg1	APS					897:899	Inner Mongolia APS	882:899	Inner Mongolia APS	882:899	Inner Mongolia APS had the largest percentage of 20-40 kDa polysaccharides.
34441532	2	45	theme	differentiated	209:222	arg1	benefits					231:238	differentiated health benefits	209:238	differentiated health benefits	209:238	It presents differentiated health benefits due to origins.
34441532	1	46	theme	functional	152:161	arg1	food					163:166	a functional food	150:166	a functional food with multiple bioactivities	150:194	Astragalus membranaceus is a functional food with multiple bioactivities.
34441532	1	46	theme	functional	152:161	arg1	membranaceus					134:145	Astragalus membranaceus	123:145	Astragalus membranaceus	123:145	Astragalus membranaceus is a functional food with multiple bioactivities.
34441532	12	47	theme	origin-related	1126:1139	arg1	quality					1141:1147	the origin-related quality	1122:1147	the origin-related quality of A. membranaceus	1122:1166	These results were helpful to recognize the origin-related quality of A. membranaceus.
34441532	7	48	theme	Rhamnogalacturonan	667:684	arg1	polysaccharides					734:748	the dominant polysaccharides	721:748	the dominant polysaccharides	721:748	Rhamnogalacturonan II pectins and α-(1→4)-glucan were the dominant polysaccharides.
34441532	7	48	theme	Rhamnogalacturonan	667:684	arg1	pectins					689:695	Rhamnogalacturonan II pectins	667:695	Rhamnogalacturonan II pectins	667:695	Rhamnogalacturonan II pectins and α-(1→4)-glucan were the dominant polysaccharides.
34441532	7	48	theme	Rhamnogalacturonan	667:684	arg1	-glucan					708:714	α-(1→4)-glucan	701:714	α-(1→4)-glucan	701:714	Rhamnogalacturonan II pectins and α-(1→4)-glucan were the dominant polysaccharides.
34441532	10	49	theme	Mongolia	888:895	arg1	APS					897:899	Inner Mongolia APS	882:899	Inner Mongolia APS	882:899	Inner Mongolia APS had the largest percentage of 20-40 kDa polysaccharides.
34441532	6	50	theme	glycosidic	647:656	arg1	linkage					658:664	glycosidic linkage	647:664	glycosidic linkage	647:664	The results showed APSs of four origins had identical monosaccharide composition and glycosidic linkage.
34441532	8	51	theme	ester	780:784	arg1	level					764:768	the level	760:768	the level of methyl ester in pectins	760:795	However, the level of methyl ester in pectins varied to a large extent.
33099185	4	0	from	qRT-PCR	519:525	arg1	samples					573:579	formalin-fixed and paraffin-embedded tumor samples	530:579	formalin-fixed and paraffin-embedded tumor samples (n = 105; 74% male patients and 26% female patients; median age = 72 years)	530:655	mRNA expression was analyzed using qRT-PCR in formalin-fixed and paraffin-embedded tumor samples (n = 105; 74% male patients and 26% female patients; median age = 72 years), correlated with histopathological variables, and evaluated by means of multivariable Cox regression analysis regarding to overall survival (OS), cancer-specific survival (CSS), and disease-free survival (DFS).
33099185	7	1	dep	B4GALT1	1432:1438	arg1	CSS					1440:1442	CSS p = 0.0134	1440:1453	B4GALT1:CSS p = 0.0134; DFS p = 0.0493	1432:1469	Low mRNA levels predicted poor survival according to the Kaplan-Meier analysis ((POFUT1:OS p = 0.0014; CSS p = 0.0007; DFS p = 0.0088); (EXT1:OS p = 0.0150; CSS p = 0.0130; DFS p = 0.0286); (B4GALT1:CSS p = 0.0134; DFS p = 0.0493)).
33099185	7	1	dep	B4GALT1	1432:1438	arg1	DFS					1456:1458	DFS p = 0.0493	1456:1469	B4GALT1:CSS p = 0.0134; DFS p = 0.0493	1432:1469	Low mRNA levels predicted poor survival according to the Kaplan-Meier analysis ((POFUT1:OS p = 0.0014; CSS p = 0.0007; DFS p = 0.0088); (EXT1:OS p = 0.0150; CSS p = 0.0130; DFS p = 0.0286); (B4GALT1:CSS p = 0.0134; DFS p = 0.0493)).
33099185	6	2	theme	cancer-specific	1165:1179	arg1	death					1181:1185	cancer-specific death	1165:1185	cancer-specific death according to the multivariable analysis (p = 0.0001)	1165:1238	Thus, the patients with low POFUT1 mRNA were at a 4.9-fold greater risk for cancer-specific death according to the multivariable analysis (p = 0.0001).
33099185	7	3	dep	EXT1	1378:1381	arg1	OS					1383:1384	OS p = 0.0150	1383:1395	OS p = 0.0150; CSS p = 0.0130; DFS p = 0.0286	1383:1427	Low mRNA levels predicted poor survival according to the Kaplan-Meier analysis ((POFUT1:OS p = 0.0014; CSS p = 0.0007; DFS p = 0.0088); (EXT1:OS p = 0.0150; CSS p = 0.0130; DFS p = 0.0286); (B4GALT1:CSS p = 0.0134; DFS p = 0.0493)).
33099185	9	4	from	OS	1735:1736	arg1	patients					1754:1761	MIBC patients	1749:1761	MIBC patients following radical cystectomy	1749:1790	Low levels of POFUT1 mRNA are an independent prognostic indicator for OS and CSS in MIBC patients following radical cystectomy.
33099185	2	5	theme	glycosyltransferases	270:289	arg1	changes					219:225	changes	219:225	changes in glycan structures	219:246	Progression is linked to changes in glycan structures and altered levels of glycosyltransferases.
33099185	2	5	theme	glycosyltransferases	270:289	arg1	levels					260:265	altered levels	252:265	altered levels of glycosyltransferases	252:289	Progression is linked to changes in glycan structures and altered levels of glycosyltransferases.
33099185	1	6	theme	rapid	175:179	arg1	progression					181:191	rapid progression	175:191	rapid progression	175:191	Muscle-invasive bladder cancer (MIBC) is characterized by high recurrence and rapid progression.
33099185	7	7	theme	CSS	1398:1400	arg1	p = 0.0130					1402:1411	CSS p = 0.0130	1398:1411	OS p = 0.0150; CSS p = 0.0130; DFS p = 0.0286	1383:1427	Low mRNA levels predicted poor survival according to the Kaplan-Meier analysis ((POFUT1:OS p = 0.0014; CSS p = 0.0007; DFS p = 0.0088); (EXT1:OS p = 0.0150; CSS p = 0.0130; DFS p = 0.0286); (B4GALT1:CSS p = 0.0134; DFS p = 0.0493)).
33099185	4	8	theme	disease-free	839:850	arg1	DFS					862:864	DFS	862:864	DFS	862:864	mRNA expression was analyzed using qRT-PCR in formalin-fixed and paraffin-embedded tumor samples (n = 105; 74% male patients and 26% female patients; median age = 72 years), correlated with histopathological variables, and evaluated by means of multivariable Cox regression analysis regarding to overall survival (OS), cancer-specific survival (CSS), and disease-free survival (DFS).
33099185	4	8	theme	disease-free	839:850	arg1	survival					852:859	disease-free survival	839:859	disease-free survival (DFS)	839:865	mRNA expression was analyzed using qRT-PCR in formalin-fixed and paraffin-embedded tumor samples (n = 105; 74% male patients and 26% female patients; median age = 72 years), correlated with histopathological variables, and evaluated by means of multivariable Cox regression analysis regarding to overall survival (OS), cancer-specific survival (CSS), and disease-free survival (DFS).
33099185	9	9	theme	radical	1773:1779	arg1	cystectomy					1781:1790	radical cystectomy	1773:1790	radical cystectomy	1773:1790	Low levels of POFUT1 mRNA are an independent prognostic indicator for OS and CSS in MIBC patients following radical cystectomy.
33099185	7	10	dep	DFS	1360:1362	arg1	EXT1					1378:1381	EXT1	1378:1381	EXT1	1378:1381	Low mRNA levels predicted poor survival according to the Kaplan-Meier analysis ((POFUT1:OS p = 0.0014; CSS p = 0.0007; DFS p = 0.0088); (EXT1:OS p = 0.0150; CSS p = 0.0130; DFS p = 0.0286); (B4GALT1:CSS p = 0.0134; DFS p = 0.0493)).
33099185	4	11	dep	%	593:593	arg1	patients					624:631	26% female patients	613:631	26% female patients	613:631	mRNA expression was analyzed using qRT-PCR in formalin-fixed and paraffin-embedded tumor samples (n = 105; 74% male patients and 26% female patients; median age = 72 years), correlated with histopathological variables, and evaluated by means of multivariable Cox regression analysis regarding to overall survival (OS), cancer-specific survival (CSS), and disease-free survival (DFS).
33099185	4	11	dep	%	593:593	arg1	patients					600:607	male patients	595:607	male patients	595:607	mRNA expression was analyzed using qRT-PCR in formalin-fixed and paraffin-embedded tumor samples (n = 105; 74% male patients and 26% female patients; median age = 72 years), correlated with histopathological variables, and evaluated by means of multivariable Cox regression analysis regarding to overall survival (OS), cancer-specific survival (CSS), and disease-free survival (DFS).
33099185	4	11	dep	%	593:593	arg1	age = 72 years					641:654	median age = 72 years	634:654	n = 105; 74% male patients and 26% female patients; median age = 72 years	582:654	mRNA expression was analyzed using qRT-PCR in formalin-fixed and paraffin-embedded tumor samples (n = 105; 74% male patients and 26% female patients; median age = 72 years), correlated with histopathological variables, and evaluated by means of multivariable Cox regression analysis regarding to overall survival (OS), cancer-specific survival (CSS), and disease-free survival (DFS).
33099185	2	12	theme	glycan	230:235	arg1	structures					237:246	glycan structures	230:246	glycan structures	230:246	Progression is linked to changes in glycan structures and altered levels of glycosyltransferases.
33099185	3	13	theme	expression	317:326	arg1	relationship					296:307	The relationship	292:307	The relationship of mRNA expression by glycosyltransferase genes B4GALT1, EXT1, MGAT5B, and POFUT1 to the probability of surviving MIBC after radical cystectomy	292:451	The relationship of mRNA expression by glycosyltransferase genes B4GALT1, EXT1, MGAT5B, and POFUT1 to the probability of surviving MIBC after radical cystectomy has not yet been investigated.
33099185	10	14	theme	glycosylation	1845:1857	arg1	importance					1823:1832	the importance	1819:1832	the importance of altered glycosylation for the progress of MIBC	1819:1882	This finding demonstrates the importance of altered glycosylation for the progress of MIBC.
33099185	5	15	theme	superior	943:950	arg1	marker					963:968	superior prognostic marker	943:968	superior prognostic marker	943:968	Multivariable Cox regression analysis identified POFUT1 mRNA expression as superior prognostic marker, compared with currently used histological tumor stage methods, for CSS by MIBC patients following radical cystectomy.
33099185	5	16	theme	MIBC	1045:1048	arg1	patients					1050:1057	MIBC patients	1045:1057	MIBC patients following radical cystectomy	1045:1086	Multivariable Cox regression analysis identified POFUT1 mRNA expression as superior prognostic marker, compared with currently used histological tumor stage methods, for CSS by MIBC patients following radical cystectomy.
33099185	9	17	from	CSS	1742:1744	arg1	patients					1754:1761	MIBC patients	1749:1761	MIBC patients following radical cystectomy	1749:1790	Low levels of POFUT1 mRNA are an independent prognostic indicator for OS and CSS in MIBC patients following radical cystectomy.
33099185	5	18	theme	mRNA	924:927	arg1	expression					929:938	POFUT1 mRNA expression	917:938	POFUT1 mRNA expression	917:938	Multivariable Cox regression analysis identified POFUT1 mRNA expression as superior prognostic marker, compared with currently used histological tumor stage methods, for CSS by MIBC patients following radical cystectomy.
33099185	5	19	theme	POFUT1	917:922	arg1	expression					929:938	POFUT1 mRNA expression	917:938	POFUT1 mRNA expression	917:938	Multivariable Cox regression analysis identified POFUT1 mRNA expression as superior prognostic marker, compared with currently used histological tumor stage methods, for CSS by MIBC patients following radical cystectomy.
33099185	5	20	theme	Multivariable	868:880	arg1	analysis					897:904	Multivariable Cox regression analysis	868:904	Multivariable Cox regression analysis	868:904	Multivariable Cox regression analysis identified POFUT1 mRNA expression as superior prognostic marker, compared with currently used histological tumor stage methods, for CSS by MIBC patients following radical cystectomy.
33099185	3	21	theme	glycosyltransferase	331:349	arg1	B4GALT1					357:363	B4GALT1	357:363	B4GALT1	357:363	The relationship of mRNA expression by glycosyltransferase genes B4GALT1, EXT1, MGAT5B, and POFUT1 to the probability of surviving MIBC after radical cystectomy has not yet been investigated.
33099185	3	21	theme	glycosyltransferase	331:349	arg1	MGAT5B					372:377	MGAT5B	372:377	MGAT5B	372:377	The relationship of mRNA expression by glycosyltransferase genes B4GALT1, EXT1, MGAT5B, and POFUT1 to the probability of surviving MIBC after radical cystectomy has not yet been investigated.
33099185	3	21	theme	glycosyltransferase	331:349	arg1	genes					351:355	glycosyltransferase genes B4GALT1, EXT1, MGAT5B, and POFUT1	331:389	glycosyltransferase genes B4GALT1, EXT1, MGAT5B, and POFUT1	331:389	The relationship of mRNA expression by glycosyltransferase genes B4GALT1, EXT1, MGAT5B, and POFUT1 to the probability of surviving MIBC after radical cystectomy has not yet been investigated.
33099185	3	21	theme	glycosyltransferase	331:349	arg1	EXT1					366:369	EXT1	366:369	EXT1	366:369	The relationship of mRNA expression by glycosyltransferase genes B4GALT1, EXT1, MGAT5B, and POFUT1 to the probability of surviving MIBC after radical cystectomy has not yet been investigated.
33099185	3	21	theme	glycosyltransferase	331:349	arg1	POFUT1					384:389	POFUT1	384:389	POFUT1	384:389	The relationship of mRNA expression by glycosyltransferase genes B4GALT1, EXT1, MGAT5B, and POFUT1 to the probability of surviving MIBC after radical cystectomy has not yet been investigated.
33099185	0	22	theme	bladder	81:87	arg1	cancer					89:94	muscle-invasive bladder cancer	65:94	muscle-invasive bladder cancer	65:94	POFUT1 mRNA expression as an independent prognostic parameter in muscle-invasive bladder cancer.
33099185	8	23	dep	pN-	1537:1539	arg1	n = 73					1542:1547	n = 73	1542:1547	pN-; n = 73	1537:1547	A subgroup analysis of patients without lymph node metastasis (pN-; n = 73) indicated that low expression of POFUT1 predicted reduced OS (p = 0.0073), CSS (p = 0.0058,) and DSS (p = 0.0079).
33099185	5	24	theme	regression	886:895	arg1	analysis					897:904	Multivariable Cox regression analysis	868:904	Multivariable Cox regression analysis	868:904	Multivariable Cox regression analysis identified POFUT1 mRNA expression as superior prognostic marker, compared with currently used histological tumor stage methods, for CSS by MIBC patients following radical cystectomy.
33099185	8	25	dep	CSS	1625:1627	arg1	p = 0.0058					1630:1639	p = 0.0058	1630:1639	p = 0.0058	1630:1639	A subgroup analysis of patients without lymph node metastasis (pN-; n = 73) indicated that low expression of POFUT1 predicted reduced OS (p = 0.0073), CSS (p = 0.0058,) and DSS (p = 0.0079).
33099185	8	26	dep	metastasis	1525:1534	arg1	pN-					1537:1539	pN-	1537:1539	pN-; n = 73	1537:1547	A subgroup analysis of patients without lymph node metastasis (pN-; n = 73) indicated that low expression of POFUT1 predicted reduced OS (p = 0.0073), CSS (p = 0.0058,) and DSS (p = 0.0079).
33099185	3	27	theme	surviving	413:421	arg1	MIBC					423:426	surviving MIBC	413:426	surviving MIBC	413:426	The relationship of mRNA expression by glycosyltransferase genes B4GALT1, EXT1, MGAT5B, and POFUT1 to the probability of surviving MIBC after radical cystectomy has not yet been investigated.
33099185	4	28	theme	Cox	743:745	arg1	analysis					758:765	multivariable Cox regression analysis	729:765	multivariable Cox regression analysis regarding to overall survival (OS), cancer-specific survival (CSS), and disease-free survival (DFS)	729:865	mRNA expression was analyzed using qRT-PCR in formalin-fixed and paraffin-embedded tumor samples (n = 105; 74% male patients and 26% female patients; median age = 72 years), correlated with histopathological variables, and evaluated by means of multivariable Cox regression analysis regarding to overall survival (OS), cancer-specific survival (CSS), and disease-free survival (DFS).
33099185	1	29	theme	Muscle-invasive	97:111	arg1	MIBC					129:132	MIBC	129:132	MIBC	129:132	Muscle-invasive bladder cancer (MIBC) is characterized by high recurrence and rapid progression.
33099185	1	29	theme	Muscle-invasive	97:111	arg1	cancer					121:126	Muscle-invasive bladder cancer	97:126	Muscle-invasive bladder cancer (MIBC)	97:133	Muscle-invasive bladder cancer (MIBC) is characterized by high recurrence and rapid progression.
33099185	5	30	theme	stage	1019:1023	arg1	methods					1025:1031	currently used histological tumor stage methods	985:1031	currently used histological tumor stage methods	985:1031	Multivariable Cox regression analysis identified POFUT1 mRNA expression as superior prognostic marker, compared with currently used histological tumor stage methods, for CSS by MIBC patients following radical cystectomy.
33099185	5	31	theme	prognostic	952:961	arg1	marker					963:968	superior prognostic marker	943:968	superior prognostic marker	943:968	Multivariable Cox regression analysis identified POFUT1 mRNA expression as superior prognostic marker, compared with currently used histological tumor stage methods, for CSS by MIBC patients following radical cystectomy.
33099185	7	32	theme	Kaplan-Meier	1298:1309	arg1	analysis					1311:1318	the Kaplan-Meier analysis	1294:1318	the Kaplan-Meier analysis ((POFUT1:OS p = 0.0014; CSS p = 0.0007; DFS p = 0.0088); (EXT1:OS p = 0.0150; CSS p = 0.0130; DFS p = 0.0286); (B4GALT1:CSS p = 0.0134; DFS p = 0.0493))	1294:1471	Low mRNA levels predicted poor survival according to the Kaplan-Meier analysis ((POFUT1:OS p = 0.0014; CSS p = 0.0007; DFS p = 0.0088); (EXT1:OS p = 0.0150; CSS p = 0.0130; DFS p = 0.0286); (B4GALT1:CSS p = 0.0134; DFS p = 0.0493)).
33099185	9	33	theme	POFUT1	1679:1684	arg1	mRNA					1686:1689	POFUT1 mRNA	1679:1689	POFUT1 mRNA	1679:1689	Low levels of POFUT1 mRNA are an independent prognostic indicator for OS and CSS in MIBC patients following radical cystectomy.
33099185	9	34	theme	mRNA	1686:1689	arg1	levels					1669:1674	Low levels	1665:1674	Low levels of POFUT1 mRNA	1665:1689	Low levels of POFUT1 mRNA are an independent prognostic indicator for OS and CSS in MIBC patients following radical cystectomy.
33099185	9	34	theme	mRNA	1686:1689	arg1	indicator					1721:1729	an independent prognostic indicator	1695:1729	an independent prognostic indicator for OS and CSS in MIBC patients following radical cystectomy	1695:1790	Low levels of POFUT1 mRNA are an independent prognostic indicator for OS and CSS in MIBC patients following radical cystectomy.
33099185	8	35	theme	subgroup	1476:1483	arg1	analysis					1485:1492	A subgroup analysis	1474:1492	A subgroup analysis of patients without lymph node metastasis (pN-; n = 73)	1474:1548	A subgroup analysis of patients without lymph node metastasis (pN-; n = 73) indicated that low expression of POFUT1 predicted reduced OS (p = 0.0073), CSS (p = 0.0058,) and DSS (p = 0.0079).
33099185	0	36	theme	prognostic	41:50	arg1	parameter					52:60	an independent prognostic parameter	26:60	an independent prognostic parameter in muscle-invasive bladder cancer	26:94	POFUT1 mRNA expression as an independent prognostic parameter in muscle-invasive bladder cancer.
33099185	7	37	dep	POFUT1	1322:1327	arg1	p = 0.0014					1332:1341	OS p = 0.0014	1329:1341	POFUT1:OS p = 0.0014; CSS p = 0.0007; DFS p = 0.0088); (EXT1:OS p = 0.0150; CSS p = 0.0130; DFS p = 0.0286)	1322:1428	Low mRNA levels predicted poor survival according to the Kaplan-Meier analysis ((POFUT1:OS p = 0.0014; CSS p = 0.0007; DFS p = 0.0088); (EXT1:OS p = 0.0150; CSS p = 0.0130; DFS p = 0.0286); (B4GALT1:CSS p = 0.0134; DFS p = 0.0493)).
33099185	7	37	dep	POFUT1	1322:1327	arg1	B4GALT1					1432:1438	B4GALT1	1432:1438	B4GALT1:CSS p = 0.0134; DFS p = 0.0493	1432:1469	Low mRNA levels predicted poor survival according to the Kaplan-Meier analysis ((POFUT1:OS p = 0.0014; CSS p = 0.0007; DFS p = 0.0088); (EXT1:OS p = 0.0150; CSS p = 0.0130; DFS p = 0.0286); (B4GALT1:CSS p = 0.0134; DFS p = 0.0493)).
33099185	7	37	dep	POFUT1	1322:1327	arg1	CSS					1344:1346	CSS p = 0.0007	1344:1357	POFUT1:OS p = 0.0014; CSS p = 0.0007; DFS p = 0.0088); (EXT1:OS p = 0.0150; CSS p = 0.0130; DFS p = 0.0286)	1322:1428	Low mRNA levels predicted poor survival according to the Kaplan-Meier analysis ((POFUT1:OS p = 0.0014; CSS p = 0.0007; DFS p = 0.0088); (EXT1:OS p = 0.0150; CSS p = 0.0130; DFS p = 0.0286); (B4GALT1:CSS p = 0.0134; DFS p = 0.0493)).
33099185	7	37	dep	POFUT1	1322:1327	arg1	DFS					1360:1362	DFS p = 0.0088);	1360:1375	POFUT1:OS p = 0.0014; CSS p = 0.0007; DFS p = 0.0088); (EXT1:OS p = 0.0150; CSS p = 0.0130; DFS p = 0.0286)	1322:1428	Low mRNA levels predicted poor survival according to the Kaplan-Meier analysis ((POFUT1:OS p = 0.0014; CSS p = 0.0007; DFS p = 0.0088); (EXT1:OS p = 0.0150; CSS p = 0.0130; DFS p = 0.0286); (B4GALT1:CSS p = 0.0134; DFS p = 0.0493)).
33099185	6	38	theme	POFUT1	1117:1122	arg1	mRNA					1124:1127	low POFUT1 mRNA	1113:1127	low POFUT1 mRNA	1113:1127	Thus, the patients with low POFUT1 mRNA were at a 4.9-fold greater risk for cancer-specific death according to the multivariable analysis (p = 0.0001).
33099185	9	39	theme	independent	1698:1708	arg1	indicator					1721:1729	an independent prognostic indicator	1695:1729	an independent prognostic indicator for OS and CSS in MIBC patients following radical cystectomy	1695:1790	Low levels of POFUT1 mRNA are an independent prognostic indicator for OS and CSS in MIBC patients following radical cystectomy.
33099185	9	39	theme	independent	1698:1708	arg1	levels					1669:1674	Low levels	1665:1674	Low levels of POFUT1 mRNA	1665:1689	Low levels of POFUT1 mRNA are an independent prognostic indicator for OS and CSS in MIBC patients following radical cystectomy.
33099185	4	40	theme	paraffin-embedded	549:565	arg1	samples					573:579	formalin-fixed and paraffin-embedded tumor samples	530:579	formalin-fixed and paraffin-embedded tumor samples (n = 105; 74% male patients and 26% female patients; median age = 72 years)	530:655	mRNA expression was analyzed using qRT-PCR in formalin-fixed and paraffin-embedded tumor samples (n = 105; 74% male patients and 26% female patients; median age = 72 years), correlated with histopathological variables, and evaluated by means of multivariable Cox regression analysis regarding to overall survival (OS), cancer-specific survival (CSS), and disease-free survival (DFS).
33099185	5	41	theme	used	995:998	arg1	methods					1025:1031	currently used histological tumor stage methods	985:1031	currently used histological tumor stage methods	985:1031	Multivariable Cox regression analysis identified POFUT1 mRNA expression as superior prognostic marker, compared with currently used histological tumor stage methods, for CSS by MIBC patients following radical cystectomy.
33099185	4	42	theme	histopathological	674:690	arg1	variables					692:700	histopathological variables	674:700	histopathological variables	674:700	mRNA expression was analyzed using qRT-PCR in formalin-fixed and paraffin-embedded tumor samples (n = 105; 74% male patients and 26% female patients; median age = 72 years), correlated with histopathological variables, and evaluated by means of multivariable Cox regression analysis regarding to overall survival (OS), cancer-specific survival (CSS), and disease-free survival (DFS).
33099185	5	43	theme	tumor	1013:1017	arg1	methods					1025:1031	currently used histological tumor stage methods	985:1031	currently used histological tumor stage methods	985:1031	Multivariable Cox regression analysis identified POFUT1 mRNA expression as superior prognostic marker, compared with currently used histological tumor stage methods, for CSS by MIBC patients following radical cystectomy.
33099185	7	44	theme	mRNA	1245:1248	arg1	levels					1250:1255	Low mRNA levels	1241:1255	Low mRNA levels	1241:1255	Low mRNA levels predicted poor survival according to the Kaplan-Meier analysis ((POFUT1:OS p = 0.0014; CSS p = 0.0007; DFS p = 0.0088); (EXT1:OS p = 0.0150; CSS p = 0.0130; DFS p = 0.0286); (B4GALT1:CSS p = 0.0134; DFS p = 0.0493)).
33099185	8	45	theme	node	1520:1523	arg1	metastasis					1525:1534	lymph node metastasis	1514:1534	lymph node metastasis (pN-; n = 73)	1514:1548	A subgroup analysis of patients without lymph node metastasis (pN-; n = 73) indicated that low expression of POFUT1 predicted reduced OS (p = 0.0073), CSS (p = 0.0058,) and DSS (p = 0.0079).
33099185	8	46	theme	POFUT1	1583:1588	arg1	expression					1569:1578	low expression	1565:1578	low expression of POFUT1	1565:1588	A subgroup analysis of patients without lymph node metastasis (pN-; n = 73) indicated that low expression of POFUT1 predicted reduced OS (p = 0.0073), CSS (p = 0.0058,) and DSS (p = 0.0079).
33099185	5	47	theme	Cox	882:884	arg1	analysis					897:904	Multivariable Cox regression analysis	868:904	Multivariable Cox regression analysis	868:904	Multivariable Cox regression analysis identified POFUT1 mRNA expression as superior prognostic marker, compared with currently used histological tumor stage methods, for CSS by MIBC patients following radical cystectomy.
33099185	9	48	theme	MIBC	1749:1752	arg1	patients					1754:1761	MIBC patients	1749:1761	MIBC patients following radical cystectomy	1749:1790	Low levels of POFUT1 mRNA are an independent prognostic indicator for OS and CSS in MIBC patients following radical cystectomy.
33099185	4	49	theme	male	595:598	arg1	patients					600:607	male patients	595:607	male patients	595:607	mRNA expression was analyzed using qRT-PCR in formalin-fixed and paraffin-embedded tumor samples (n = 105; 74% male patients and 26% female patients; median age = 72 years), correlated with histopathological variables, and evaluated by means of multivariable Cox regression analysis regarding to overall survival (OS), cancer-specific survival (CSS), and disease-free survival (DFS).
33099185	8	50	theme	reduced	1600:1606	arg1	p = 0.0073					1612:1621	p = 0.0073	1612:1621	p = 0.0073	1612:1621	A subgroup analysis of patients without lymph node metastasis (pN-; n = 73) indicated that low expression of POFUT1 predicted reduced OS (p = 0.0073), CSS (p = 0.0058,) and DSS (p = 0.0079).
33099185	8	50	theme	reduced	1600:1606	arg1	OS					1608:1609	reduced OS	1600:1609	reduced OS (p = 0.0073)	1600:1622	A subgroup analysis of patients without lymph node metastasis (pN-; n = 73) indicated that low expression of POFUT1 predicted reduced OS (p = 0.0073), CSS (p = 0.0058,) and DSS (p = 0.0079).
33099185	6	51	with	patients	1099:1106	arg1	mRNA					1124:1127	low POFUT1 mRNA	1113:1127	low POFUT1 mRNA	1113:1127	Thus, the patients with low POFUT1 mRNA were at a 4.9-fold greater risk for cancer-specific death according to the multivariable analysis (p = 0.0001).
33099185	6	52	theme	greater	1148:1154	arg1	risk					1156:1159	a 4.9-fold greater risk	1137:1159	a 4.9-fold greater risk for cancer-specific death according to the multivariable analysis (p = 0.0001)	1137:1238	Thus, the patients with low POFUT1 mRNA were at a 4.9-fold greater risk for cancer-specific death according to the multivariable analysis (p = 0.0001).
33099185	8	53	theme	patients	1497:1504	arg1	analysis					1485:1492	A subgroup analysis	1474:1492	A subgroup analysis of patients without lymph node metastasis (pN-; n = 73)	1474:1548	A subgroup analysis of patients without lymph node metastasis (pN-; n = 73) indicated that low expression of POFUT1 predicted reduced OS (p = 0.0073), CSS (p = 0.0058,) and DSS (p = 0.0079).
33099185	5	54	theme	radical	1069:1075	arg1	cystectomy					1077:1086	radical cystectomy	1069:1086	radical cystectomy	1069:1086	Multivariable Cox regression analysis identified POFUT1 mRNA expression as superior prognostic marker, compared with currently used histological tumor stage methods, for CSS by MIBC patients following radical cystectomy.
33099185	4	55	theme	median	634:639	arg1	age = 72 years					641:654	median age = 72 years	634:654	n = 105; 74% male patients and 26% female patients; median age = 72 years	582:654	mRNA expression was analyzed using qRT-PCR in formalin-fixed and paraffin-embedded tumor samples (n = 105; 74% male patients and 26% female patients; median age = 72 years), correlated with histopathological variables, and evaluated by means of multivariable Cox regression analysis regarding to overall survival (OS), cancer-specific survival (CSS), and disease-free survival (DFS).
33099185	4	56	dep	analysis	758:765	arg1	means					720:724	means	720:724	means	720:724	mRNA expression was analyzed using qRT-PCR in formalin-fixed and paraffin-embedded tumor samples (n = 105; 74% male patients and 26% female patients; median age = 72 years), correlated with histopathological variables, and evaluated by means of multivariable Cox regression analysis regarding to overall survival (OS), cancer-specific survival (CSS), and disease-free survival (DFS).
33099185	6	57	theme	multivariable	1204:1216	arg1	analysis					1218:1225	the multivariable analysis	1200:1225	the multivariable analysis (p = 0.0001)	1200:1238	Thus, the patients with low POFUT1 mRNA were at a 4.9-fold greater risk for cancer-specific death according to the multivariable analysis (p = 0.0001).
33099185	6	57	theme	multivariable	1204:1216	arg1	p = 0.0001					1228:1237	p = 0.0001	1228:1237	p = 0.0001	1228:1237	Thus, the patients with low POFUT1 mRNA were at a 4.9-fold greater risk for cancer-specific death according to the multivariable analysis (p = 0.0001).
33099185	10	58	theme	altered	1837:1843	arg1	glycosylation					1845:1857	altered glycosylation	1837:1857	altered glycosylation	1837:1857	This finding demonstrates the importance of altered glycosylation for the progress of MIBC.
33099185	7	59	dep	OS	1383:1384	arg1	p = 0.0130					1402:1411	CSS p = 0.0130	1398:1411	OS p = 0.0150; CSS p = 0.0130; DFS p = 0.0286	1383:1427	Low mRNA levels predicted poor survival according to the Kaplan-Meier analysis ((POFUT1:OS p = 0.0014; CSS p = 0.0007; DFS p = 0.0088); (EXT1:OS p = 0.0150; CSS p = 0.0130; DFS p = 0.0286); (B4GALT1:CSS p = 0.0134; DFS p = 0.0493)).
33099185	7	59	dep	OS	1383:1384	arg1	DFS					1414:1416	DFS p = 0.0286	1414:1427	OS p = 0.0150; CSS p = 0.0130; DFS p = 0.0286	1383:1427	Low mRNA levels predicted poor survival according to the Kaplan-Meier analysis ((POFUT1:OS p = 0.0014; CSS p = 0.0007; DFS p = 0.0088); (EXT1:OS p = 0.0150; CSS p = 0.0130; DFS p = 0.0286); (B4GALT1:CSS p = 0.0134; DFS p = 0.0493)).
33099185	3	60	theme	mRNA	312:315	arg1	expression					317:326	mRNA expression	312:326	mRNA expression by glycosyltransferase genes B4GALT1, EXT1, MGAT5B, and POFUT1	312:389	The relationship of mRNA expression by glycosyltransferase genes B4GALT1, EXT1, MGAT5B, and POFUT1 to the probability of surviving MIBC after radical cystectomy has not yet been investigated.
33099185	10	61	theme	MIBC	1879:1882	arg1	progress					1867:1874	the progress	1863:1874	the progress of MIBC	1863:1882	This finding demonstrates the importance of altered glycosylation for the progress of MIBC.
33099185	0	62	theme	muscle-invasive	65:79	arg1	cancer					89:94	muscle-invasive bladder cancer	65:94	muscle-invasive bladder cancer	65:94	POFUT1 mRNA expression as an independent prognostic parameter in muscle-invasive bladder cancer.
33099185	2	63	theme	altered	252:258	arg1	levels					260:265	altered levels	252:265	altered levels of glycosyltransferases	252:289	Progression is linked to changes in glycan structures and altered levels of glycosyltransferases.
33099185	7	64	theme	OS	1329:1330	arg1	p = 0.0014					1332:1341	OS p = 0.0014	1329:1341	POFUT1:OS p = 0.0014; CSS p = 0.0007; DFS p = 0.0088); (EXT1:OS p = 0.0150; CSS p = 0.0130; DFS p = 0.0286)	1322:1428	Low mRNA levels predicted poor survival according to the Kaplan-Meier analysis ((POFUT1:OS p = 0.0014; CSS p = 0.0007; DFS p = 0.0088); (EXT1:OS p = 0.0150; CSS p = 0.0130; DFS p = 0.0286); (B4GALT1:CSS p = 0.0134; DFS p = 0.0493)).
33099185	8	65	theme	lymph	1514:1518	arg1	node					1520:1523	lymph node	1514:1523	lymph node metastasis (pN-; n = 73)	1514:1548	A subgroup analysis of patients without lymph node metastasis (pN-; n = 73) indicated that low expression of POFUT1 predicted reduced OS (p = 0.0073), CSS (p = 0.0058,) and DSS (p = 0.0079).
33099185	1	66	theme	high	155:158	arg1	recurrence					160:169	high recurrence	155:169	high recurrence	155:169	Muscle-invasive bladder cancer (MIBC) is characterized by high recurrence and rapid progression.
33099185	0	67	from	parameter	52:60	arg1	cancer					89:94	muscle-invasive bladder cancer	65:94	muscle-invasive bladder cancer	65:94	POFUT1 mRNA expression as an independent prognostic parameter in muscle-invasive bladder cancer.
33099185	4	68	theme	mRNA	484:487	arg1	expression					489:498	mRNA expression	484:498	mRNA expression	484:498	mRNA expression was analyzed using qRT-PCR in formalin-fixed and paraffin-embedded tumor samples (n = 105; 74% male patients and 26% female patients; median age = 72 years), correlated with histopathological variables, and evaluated by means of multivariable Cox regression analysis regarding to overall survival (OS), cancer-specific survival (CSS), and disease-free survival (DFS).
33099185	2	69	from	changes	219:225	arg1	structures					237:246	glycan structures	230:246	glycan structures	230:246	Progression is linked to changes in glycan structures and altered levels of glycosyltransferases.
33099185	2	70	attach	linked	209:214	arg1	changes					219:225	changes	219:225	changes in glycan structures	219:246	Progression is linked to changes in glycan structures and altered levels of glycosyltransferases.
33099185	2	70	attach	linked	209:214	arg1	levels					260:265	altered levels	252:265	altered levels of glycosyltransferases	252:289	Progression is linked to changes in glycan structures and altered levels of glycosyltransferases.
33099185	2	70	attach	linked	209:214	arg2	Progression					194:204	Progression	194:204	Progression	194:204	Progression is linked to changes in glycan structures and altered levels of glycosyltransferases.
33099185	4	71	theme	overall	780:786	arg1	OS					798:799	OS	798:799	OS	798:799	mRNA expression was analyzed using qRT-PCR in formalin-fixed and paraffin-embedded tumor samples (n = 105; 74% male patients and 26% female patients; median age = 72 years), correlated with histopathological variables, and evaluated by means of multivariable Cox regression analysis regarding to overall survival (OS), cancer-specific survival (CSS), and disease-free survival (DFS).
33099185	4	71	theme	overall	780:786	arg1	survival					788:795	overall survival	780:795	overall survival (OS)	780:800	mRNA expression was analyzed using qRT-PCR in formalin-fixed and paraffin-embedded tumor samples (n = 105; 74% male patients and 26% female patients; median age = 72 years), correlated with histopathological variables, and evaluated by means of multivariable Cox regression analysis regarding to overall survival (OS), cancer-specific survival (CSS), and disease-free survival (DFS).
33099185	4	72	dep	samples	573:579	arg1	%					593:593	n = 105; 74%	582:593	n = 105; 74% male patients and 26% female patients; median age = 72 years	582:654	mRNA expression was analyzed using qRT-PCR in formalin-fixed and paraffin-embedded tumor samples (n = 105; 74% male patients and 26% female patients; median age = 72 years), correlated with histopathological variables, and evaluated by means of multivariable Cox regression analysis regarding to overall survival (OS), cancer-specific survival (CSS), and disease-free survival (DFS).
33099185	9	73	theme	Low	1665:1667	arg1	levels					1669:1674	Low levels	1665:1674	Low levels of POFUT1 mRNA	1665:1689	Low levels of POFUT1 mRNA are an independent prognostic indicator for OS and CSS in MIBC patients following radical cystectomy.
33099185	9	73	theme	Low	1665:1667	arg1	indicator					1721:1729	an independent prognostic indicator	1695:1729	an independent prognostic indicator for OS and CSS in MIBC patients following radical cystectomy	1695:1790	Low levels of POFUT1 mRNA are an independent prognostic indicator for OS and CSS in MIBC patients following radical cystectomy.
33099185	4	74	theme	multivariable	729:741	arg1	analysis					758:765	multivariable Cox regression analysis	729:765	multivariable Cox regression analysis regarding to overall survival (OS), cancer-specific survival (CSS), and disease-free survival (DFS)	729:865	mRNA expression was analyzed using qRT-PCR in formalin-fixed and paraffin-embedded tumor samples (n = 105; 74% male patients and 26% female patients; median age = 72 years), correlated with histopathological variables, and evaluated by means of multivariable Cox regression analysis regarding to overall survival (OS), cancer-specific survival (CSS), and disease-free survival (DFS).
33099185	3	75	theme	radical	434:440	arg1	cystectomy					442:451	radical cystectomy	434:451	radical cystectomy	434:451	The relationship of mRNA expression by glycosyltransferase genes B4GALT1, EXT1, MGAT5B, and POFUT1 to the probability of surviving MIBC after radical cystectomy has not yet been investigated.
33099185	1	76	theme	bladder	113:119	arg1	MIBC					129:132	MIBC	129:132	MIBC	129:132	Muscle-invasive bladder cancer (MIBC) is characterized by high recurrence and rapid progression.
33099185	1	76	theme	bladder	113:119	arg1	cancer					121:126	Muscle-invasive bladder cancer	97:126	Muscle-invasive bladder cancer (MIBC)	97:133	Muscle-invasive bladder cancer (MIBC) is characterized by high recurrence and rapid progression.
33099185	3	77	dep	genes	351:355	arg1	B4GALT1					357:363	B4GALT1	357:363	B4GALT1	357:363	The relationship of mRNA expression by glycosyltransferase genes B4GALT1, EXT1, MGAT5B, and POFUT1 to the probability of surviving MIBC after radical cystectomy has not yet been investigated.
33099185	3	77	dep	genes	351:355	arg1	MGAT5B					372:377	MGAT5B	372:377	MGAT5B	372:377	The relationship of mRNA expression by glycosyltransferase genes B4GALT1, EXT1, MGAT5B, and POFUT1 to the probability of surviving MIBC after radical cystectomy has not yet been investigated.
33099185	3	77	dep	genes	351:355	arg1	POFUT1					384:389	POFUT1	384:389	POFUT1	384:389	The relationship of mRNA expression by glycosyltransferase genes B4GALT1, EXT1, MGAT5B, and POFUT1 to the probability of surviving MIBC after radical cystectomy has not yet been investigated.
33099185	3	77	dep	genes	351:355	arg1	EXT1					366:369	EXT1	366:369	EXT1	366:369	The relationship of mRNA expression by glycosyltransferase genes B4GALT1, EXT1, MGAT5B, and POFUT1 to the probability of surviving MIBC after radical cystectomy has not yet been investigated.
33099185	3	77	dep	genes	351:355	arg1	genes					351:355	glycosyltransferase genes B4GALT1, EXT1, MGAT5B, and POFUT1	331:389	glycosyltransferase genes B4GALT1, EXT1, MGAT5B, and POFUT1	331:389	The relationship of mRNA expression by glycosyltransferase genes B4GALT1, EXT1, MGAT5B, and POFUT1 to the probability of surviving MIBC after radical cystectomy has not yet been investigated.
33099185	4	78	theme	regression	747:756	arg1	analysis					758:765	multivariable Cox regression analysis	729:765	multivariable Cox regression analysis regarding to overall survival (OS), cancer-specific survival (CSS), and disease-free survival (DFS)	729:865	mRNA expression was analyzed using qRT-PCR in formalin-fixed and paraffin-embedded tumor samples (n = 105; 74% male patients and 26% female patients; median age = 72 years), correlated with histopathological variables, and evaluated by means of multivariable Cox regression analysis regarding to overall survival (OS), cancer-specific survival (CSS), and disease-free survival (DFS).
33099185	0	79	theme	independent	29:39	arg1	parameter					52:60	an independent prognostic parameter	26:60	an independent prognostic parameter in muscle-invasive bladder cancer	26:94	POFUT1 mRNA expression as an independent prognostic parameter in muscle-invasive bladder cancer.
33099185	7	80	dep	analysis	1311:1318	arg1	POFUT1					1322:1327	POFUT1	1322:1327	POFUT1:OS p = 0.0014; CSS p = 0.0007; DFS p = 0.0088); (EXT1:OS p = 0.0150; CSS p = 0.0130; DFS p = 0.0286)	1322:1428	Low mRNA levels predicted poor survival according to the Kaplan-Meier analysis ((POFUT1:OS p = 0.0014; CSS p = 0.0007; DFS p = 0.0088); (EXT1:OS p = 0.0150; CSS p = 0.0130; DFS p = 0.0286); (B4GALT1:CSS p = 0.0134; DFS p = 0.0493)).
33099185	2	81	from	levels	260:265	arg1	structures					237:246	glycan structures	230:246	glycan structures	230:246	Progression is linked to changes in glycan structures and altered levels of glycosyltransferases.
33099185	3	82	theme	MIBC	423:426	arg1	probability					398:408	the probability	394:408	the probability of surviving MIBC after radical cystectomy	394:451	The relationship of mRNA expression by glycosyltransferase genes B4GALT1, EXT1, MGAT5B, and POFUT1 to the probability of surviving MIBC after radical cystectomy has not yet been investigated.
33099185	4	83	theme	cancer-specific	803:817	arg1	survival					819:826	cancer-specific survival	803:826	cancer-specific survival (CSS)	803:832	mRNA expression was analyzed using qRT-PCR in formalin-fixed and paraffin-embedded tumor samples (n = 105; 74% male patients and 26% female patients; median age = 72 years), correlated with histopathological variables, and evaluated by means of multivariable Cox regression analysis regarding to overall survival (OS), cancer-specific survival (CSS), and disease-free survival (DFS).
33099185	4	83	theme	cancer-specific	803:817	arg1	CSS					829:831	CSS	829:831	CSS	829:831	mRNA expression was analyzed using qRT-PCR in formalin-fixed and paraffin-embedded tumor samples (n = 105; 74% male patients and 26% female patients; median age = 72 years), correlated with histopathological variables, and evaluated by means of multivariable Cox regression analysis regarding to overall survival (OS), cancer-specific survival (CSS), and disease-free survival (DFS).
33099185	7	84	theme	poor	1267:1270	arg1	survival					1272:1279	poor survival	1267:1279	poor survival	1267:1279	Low mRNA levels predicted poor survival according to the Kaplan-Meier analysis ((POFUT1:OS p = 0.0014; CSS p = 0.0007; DFS p = 0.0088); (EXT1:OS p = 0.0150; CSS p = 0.0130; DFS p = 0.0286); (B4GALT1:CSS p = 0.0134; DFS p = 0.0493)).
33099185	4	85	theme	tumor	567:571	arg1	samples					573:579	formalin-fixed and paraffin-embedded tumor samples	530:579	formalin-fixed and paraffin-embedded tumor samples (n = 105; 74% male patients and 26% female patients; median age = 72 years)	530:655	mRNA expression was analyzed using qRT-PCR in formalin-fixed and paraffin-embedded tumor samples (n = 105; 74% male patients and 26% female patients; median age = 72 years), correlated with histopathological variables, and evaluated by means of multivariable Cox regression analysis regarding to overall survival (OS), cancer-specific survival (CSS), and disease-free survival (DFS).
33099185	6	86	theme	low	1113:1115	arg1	mRNA					1124:1127	low POFUT1 mRNA	1113:1127	low POFUT1 mRNA	1113:1127	Thus, the patients with low POFUT1 mRNA were at a 4.9-fold greater risk for cancer-specific death according to the multivariable analysis (p = 0.0001).
33099185	9	87	theme	prognostic	1710:1719	arg1	indicator					1721:1729	an independent prognostic indicator	1695:1729	an independent prognostic indicator for OS and CSS in MIBC patients following radical cystectomy	1695:1790	Low levels of POFUT1 mRNA are an independent prognostic indicator for OS and CSS in MIBC patients following radical cystectomy.
33099185	9	87	theme	prognostic	1710:1719	arg1	levels					1669:1674	Low levels	1665:1674	Low levels of POFUT1 mRNA	1665:1689	Low levels of POFUT1 mRNA are an independent prognostic indicator for OS and CSS in MIBC patients following radical cystectomy.
33099185	4	88	theme	26	613:614	arg1	%					615:615	%	615:615	%	615:615	mRNA expression was analyzed using qRT-PCR in formalin-fixed and paraffin-embedded tumor samples (n = 105; 74% male patients and 26% female patients; median age = 72 years), correlated with histopathological variables, and evaluated by means of multivariable Cox regression analysis regarding to overall survival (OS), cancer-specific survival (CSS), and disease-free survival (DFS).
33099185	8	89	theme	low	1565:1567	arg1	expression					1569:1578	low expression	1565:1578	low expression of POFUT1	1565:1588	A subgroup analysis of patients without lymph node metastasis (pN-; n = 73) indicated that low expression of POFUT1 predicted reduced OS (p = 0.0073), CSS (p = 0.0058,) and DSS (p = 0.0079).
33099185	4	90	theme	formalin-fixed	530:543	arg1	samples					573:579	formalin-fixed and paraffin-embedded tumor samples	530:579	formalin-fixed and paraffin-embedded tumor samples (n = 105; 74% male patients and 26% female patients; median age = 72 years)	530:655	mRNA expression was analyzed using qRT-PCR in formalin-fixed and paraffin-embedded tumor samples (n = 105; 74% male patients and 26% female patients; median age = 72 years), correlated with histopathological variables, and evaluated by means of multivariable Cox regression analysis regarding to overall survival (OS), cancer-specific survival (CSS), and disease-free survival (DFS).
33099185	5	91	theme	histological	1000:1011	arg1	methods					1025:1031	currently used histological tumor stage methods	985:1031	currently used histological tumor stage methods	985:1031	Multivariable Cox regression analysis identified POFUT1 mRNA expression as superior prognostic marker, compared with currently used histological tumor stage methods, for CSS by MIBC patients following radical cystectomy.
33099185	7	92	theme	Low	1241:1243	arg1	levels					1250:1255	Low mRNA levels	1241:1255	Low mRNA levels	1241:1255	Low mRNA levels predicted poor survival according to the Kaplan-Meier analysis ((POFUT1:OS p = 0.0014; CSS p = 0.0007; DFS p = 0.0088); (EXT1:OS p = 0.0150; CSS p = 0.0130; DFS p = 0.0286); (B4GALT1:CSS p = 0.0134; DFS p = 0.0493)).
33099185	4	93	theme	female	617:622	arg1	patients					624:631	26% female patients	613:631	26% female patients	613:631	mRNA expression was analyzed using qRT-PCR in formalin-fixed and paraffin-embedded tumor samples (n = 105; 74% male patients and 26% female patients; median age = 72 years), correlated with histopathological variables, and evaluated by means of multivariable Cox regression analysis regarding to overall survival (OS), cancer-specific survival (CSS), and disease-free survival (DFS).
33099185	4	94	theme	%	615:615	arg1	patients					624:631	26% female patients	613:631	26% female patients	613:631	mRNA expression was analyzed using qRT-PCR in formalin-fixed and paraffin-embedded tumor samples (n = 105; 74% male patients and 26% female patients; median age = 72 years), correlated with histopathological variables, and evaluated by means of multivariable Cox regression analysis regarding to overall survival (OS), cancer-specific survival (CSS), and disease-free survival (DFS).
32750471	3	0	theme	beef	931:934	arg1	loins					936:940	beef loins	931:940	beef loins	931:940	The treatments also slowed down the increasing level of total volatile basic nitrogen and pH during storage, while irradiation increased the levels of thiobarbituric acid reactive substances and protein carbonyls in beef loins.
32750471	0	1	theme	beef	176:179	arg1	loins					181:185	beef loins	176:185	beef loins	176:185	Effect of edible composite film based on chitosan and cumin essential oil-loaded nanoemulsion combined with low-dose gamma irradiation on microbiological safety and quality of beef loins during refrigerated storage.
32750471	1	2	theme	combined	258:265	arg1	effect					267:272	the combined effect	254:272	the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins	254:443	This research was conducted to assess the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins during 21 days of chilled storage.
32750471	2	3	theme	bacteria	569:576	arg1	growth					484:489	The growth	480:489	The growth of mesophilic and psychrophilic bacteria, Enterobacteriaceae, and lactic acid bacteria	480:576	The growth of mesophilic and psychrophilic bacteria, Enterobacteriaceae, and lactic acid bacteria were retarded in all treated groups (Ch, GI, Ch + CNE, Ch + GI, and Ch + CNE + GI groups) compared to control group during storage time.
32750471	2	4	theme	storage	701:707	arg1	time					709:712	storage time	701:712	storage time	701:712	The growth of mesophilic and psychrophilic bacteria, Enterobacteriaceae, and lactic acid bacteria were retarded in all treated groups (Ch, GI, Ch + CNE, Ch + GI, and Ch + CNE + GI groups) compared to control group during storage time.
32750471	1	5	theme	beef	434:437	arg1	loins					439:443	beef loins	434:443	beef loins	434:443	This research was conducted to assess the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins during 21 days of chilled storage.
32750471	5	6	theme	loins	1359:1363	arg1	life					1346:1349	the storage life	1334:1349	the storage life of beef loins	1334:1363	The combination of Ch + CNE + GI was the most effective treatment to control the population of microbial flora and inoculated pathogens, slow down some physicochemical changes, and enhance the storage life of beef loins.
32750471	4	7	theme	Listeria	1050:1057	arg1	monocytogenes					1059:1071	Listeria monocytogenes	1050:1071	Listeria monocytogenes	1050:1071	All treatments except Ch were effective to control the growth of inoculated pathogenic bacteria, including Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella typhimurium, in loin samples.
32750471	0	8	theme	gamma	117:121	arg1	irradiation					123:133	low-dose gamma irradiation	108:133	low-dose gamma irradiation	108:133	Effect of edible composite film based on chitosan and cumin essential oil-loaded nanoemulsion combined with low-dose gamma irradiation on microbiological safety and quality of beef loins during refrigerated storage.
32750471	6	9	theme	irradiation	1438:1448	arg1	combination					1383:1393	the combination	1379:1393	the combination of active chitosan film and low-dose gamma irradiation	1379:1448	As a result, the combination of active chitosan film and low-dose gamma irradiation can ensure microbiological safety and is suggested for long time preservation of beef during chilled storage.
32750471	2	10	theme	psychrophilic	509:521	arg1	bacteria					523:530	mesophilic and psychrophilic bacteria	494:530	mesophilic and psychrophilic bacteria	494:530	The growth of mesophilic and psychrophilic bacteria, Enterobacteriaceae, and lactic acid bacteria were retarded in all treated groups (Ch, GI, Ch + CNE, Ch + GI, and Ch + CNE + GI groups) compared to control group during storage time.
32750471	2	11	dep	retarded	583:590	arg1	Ch					615:616	Ch	615:616	Ch	615:616	The growth of mesophilic and psychrophilic bacteria, Enterobacteriaceae, and lactic acid bacteria were retarded in all treated groups (Ch, GI, Ch + CNE, Ch + GI, and Ch + CNE + GI groups) compared to control group during storage time.
32750471	2	12	theme	lactic	557:562	arg1	bacteria					569:576	lactic acid bacteria	557:576	lactic acid bacteria	557:576	The growth of mesophilic and psychrophilic bacteria, Enterobacteriaceae, and lactic acid bacteria were retarded in all treated groups (Ch, GI, Ch + CNE, Ch + GI, and Ch + CNE + GI groups) compared to control group during storage time.
32750471	4	13	theme	loin	1131:1134	arg1	samples					1136:1142	loin samples	1131:1142	loin samples	1131:1142	All treatments except Ch were effective to control the growth of inoculated pathogenic bacteria, including Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella typhimurium, in loin samples.
32750471	6	14	theme	time	1510:1513	arg1	preservation					1515:1526	long time preservation	1505:1526	long time preservation of beef during chilled storage	1505:1557	As a result, the combination of active chitosan film and low-dose gamma irradiation can ensure microbiological safety and is suggested for long time preservation of beef during chilled storage.
32750471	3	15	theme	basic	786:790	arg1	nitrogen					792:799	total volatile basic nitrogen	771:799	total volatile basic nitrogen	771:799	The treatments also slowed down the increasing level of total volatile basic nitrogen and pH during storage, while irradiation increased the levels of thiobarbituric acid reactive substances and protein carbonyls in beef loins.
32750471	5	16	theme	microbial	1240:1248	arg1	flora					1250:1254	microbial flora	1240:1254	microbial flora	1240:1254	The combination of Ch + CNE + GI was the most effective treatment to control the population of microbial flora and inoculated pathogens, slow down some physicochemical changes, and enhance the storage life of beef loins.
32750471	5	16	theme	microbial	1240:1248	arg1	changes					1313:1319	some physicochemical changes	1292:1319	slow down some physicochemical changes	1282:1319	The combination of Ch + CNE + GI was the most effective treatment to control the population of microbial flora and inoculated pathogens, slow down some physicochemical changes, and enhance the storage life of beef loins.
32750471	1	17	theme	oil	323:325	arg1	CNE					341:343	CNE	341:343	CNE	341:343	This research was conducted to assess the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins during 21 days of chilled storage.
32750471	1	17	theme	oil	323:325	arg1	nanoemulsion					327:338	cumin essential oil nanoemulsion	307:338	cumin essential oil nanoemulsion (CNE)	307:344	This research was conducted to assess the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins during 21 days of chilled storage.
32750471	4	18	dep	O157	1091:1094	arg1	coli					1086:1089	Escherichia coli O157:H7	1074:1097	Escherichia coli O157:H7	1074:1097	All treatments except Ch were effective to control the growth of inoculated pathogenic bacteria, including Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella typhimurium, in loin samples.
32750471	4	18	dep	O157	1091:1094	arg1	H7					1096:1097	H7	1096:1097	Escherichia coli O157:H7	1074:1097	All treatments except Ch were effective to control the growth of inoculated pathogenic bacteria, including Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella typhimurium, in loin samples.
32750471	2	19	dep	Ch	615:616	arg1	Ch + CNE + GI					646:658	Ch + CNE + GI	646:658	Ch + CNE + GI	646:658	The growth of mesophilic and psychrophilic bacteria, Enterobacteriaceae, and lactic acid bacteria were retarded in all treated groups (Ch, GI, Ch + CNE, Ch + GI, and Ch + CNE + GI groups) compared to control group during storage time.
32750471	2	19	dep	Ch	615:616	arg1	GI					619:620	GI	619:620	GI	619:620	The growth of mesophilic and psychrophilic bacteria, Enterobacteriaceae, and lactic acid bacteria were retarded in all treated groups (Ch, GI, Ch + CNE, Ch + GI, and Ch + CNE + GI groups) compared to control group during storage time.
32750471	2	19	dep	Ch	615:616	arg1	Ch + GI					633:639	Ch + GI	633:639	Ch + GI	633:639	The growth of mesophilic and psychrophilic bacteria, Enterobacteriaceae, and lactic acid bacteria were retarded in all treated groups (Ch, GI, Ch + CNE, Ch + GI, and Ch + CNE + GI groups) compared to control group during storage time.
32750471	3	20	theme	total	771:775	arg1	nitrogen					792:799	total volatile basic nitrogen	771:799	total volatile basic nitrogen	771:799	The treatments also slowed down the increasing level of total volatile basic nitrogen and pH during storage, while irradiation increased the levels of thiobarbituric acid reactive substances and protein carbonyls in beef loins.
32750471	0	21	theme	essential	60:68	arg1	nanoemulsion					81:92	cumin essential oil-loaded nanoemulsion	54:92	cumin essential oil-loaded nanoemulsion	54:92	Effect of edible composite film based on chitosan and cumin essential oil-loaded nanoemulsion combined with low-dose gamma irradiation on microbiological safety and quality of beef loins during refrigerated storage.
32750471	2	22	dep	GI	619:620	arg1	groups					660:665	groups	660:665	groups	660:665	The growth of mesophilic and psychrophilic bacteria, Enterobacteriaceae, and lactic acid bacteria were retarded in all treated groups (Ch, GI, Ch + CNE, Ch + GI, and Ch + CNE + GI groups) compared to control group during storage time.
32750471	2	23	theme	mesophilic	494:503	arg1	bacteria					523:530	mesophilic and psychrophilic bacteria	494:530	mesophilic and psychrophilic bacteria	494:530	The growth of mesophilic and psychrophilic bacteria, Enterobacteriaceae, and lactic acid bacteria were retarded in all treated groups (Ch, GI, Ch + CNE, Ch + GI, and Ch + CNE + GI groups) compared to control group during storage time.
32750471	5	24	theme	slow	1282:1285	arg1	flora					1250:1254	microbial flora	1240:1254	microbial flora	1240:1254	The combination of Ch + CNE + GI was the most effective treatment to control the population of microbial flora and inoculated pathogens, slow down some physicochemical changes, and enhance the storage life of beef loins.
32750471	5	24	theme	slow	1282:1285	arg1	changes					1313:1319	some physicochemical changes	1292:1319	slow down some physicochemical changes	1282:1319	The combination of Ch + CNE + GI was the most effective treatment to control the population of microbial flora and inoculated pathogens, slow down some physicochemical changes, and enhance the storage life of beef loins.
32750471	1	25	theme	low-dose	350:357	arg1	GI					378:379	GI	378:379	GI	378:379	This research was conducted to assess the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins during 21 days of chilled storage.
32750471	1	25	theme	low-dose	350:357	arg1	irradiation					365:375	low-dose gamma irradiation	350:375	low-dose gamma irradiation (GI)	350:380	This research was conducted to assess the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins during 21 days of chilled storage.
32750471	1	26	theme	chitosan	277:284	arg1	film					291:294	chitosan (Ch) film	277:294	chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy	277:391	This research was conducted to assess the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins during 21 days of chilled storage.
32750471	3	27	theme	acid	881:884	arg1	substances					895:904	thiobarbituric acid reactive substances	866:904	thiobarbituric acid reactive substances	866:904	The treatments also slowed down the increasing level of total volatile basic nitrogen and pH during storage, while irradiation increased the levels of thiobarbituric acid reactive substances and protein carbonyls in beef loins.
32750471	0	28	theme	edible	10:15	arg1	film					27:30	edible composite film	10:30	edible composite film	10:30	Effect of edible composite film based on chitosan and cumin essential oil-loaded nanoemulsion combined with low-dose gamma irradiation on microbiological safety and quality of beef loins during refrigerated storage.
32750471	4	29	from	effective	973:981	arg1	samples					1136:1142	loin samples	1131:1142	loin samples	1131:1142	All treatments except Ch were effective to control the growth of inoculated pathogenic bacteria, including Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella typhimurium, in loin samples.
32750471	5	30	theme	pathogens	1271:1279	arg1	population					1226:1235	the population	1222:1235	the population of microbial flora and inoculated pathogens, slow down some physicochemical changes,	1222:1320	The combination of Ch + CNE + GI was the most effective treatment to control the population of microbial flora and inoculated pathogens, slow down some physicochemical changes, and enhance the storage life of beef loins.
32750471	0	31	theme	film	27:30	arg1	Effect					0:5	Effect	0:5	Effect of edible composite film based on chitosan and cumin essential oil-loaded nanoemulsion	0:92	Effect of edible composite film based on chitosan and cumin essential oil-loaded nanoemulsion combined with low-dose gamma irradiation on microbiological safety and quality of beef loins during refrigerated storage.
32750471	1	32	theme	film	291:294	arg1	effect					267:272	the combined effect	254:272	the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins	254:443	This research was conducted to assess the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins during 21 days of chilled storage.
32750471	3	33	theme	protein	910:916	arg1	carbonyls					918:926	protein carbonyls	910:926	protein carbonyls	910:926	The treatments also slowed down the increasing level of total volatile basic nitrogen and pH during storage, while irradiation increased the levels of thiobarbituric acid reactive substances and protein carbonyls in beef loins.
32750471	3	34	from	carbonyls	918:926	arg1	loins					936:940	beef loins	931:940	beef loins	931:940	The treatments also slowed down the increasing level of total volatile basic nitrogen and pH during storage, while irradiation increased the levels of thiobarbituric acid reactive substances and protein carbonyls in beef loins.
32750471	1	35	theme	chilled	463:469	arg1	storage					471:477	chilled storage	463:477	chilled storage	463:477	This research was conducted to assess the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins during 21 days of chilled storage.
32750471	1	36	theme	cumin	307:311	arg1	CNE					341:343	CNE	341:343	CNE	341:343	This research was conducted to assess the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins during 21 days of chilled storage.
32750471	1	36	theme	cumin	307:311	arg1	nanoemulsion					327:338	cumin essential oil nanoemulsion	307:338	cumin essential oil nanoemulsion (CNE)	307:344	This research was conducted to assess the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins during 21 days of chilled storage.
32750471	3	37	theme	substances	895:904	arg1	levels					856:861	the levels	852:861	the levels of thiobarbituric acid reactive substances and protein carbonyls in beef loins	852:940	The treatments also slowed down the increasing level of total volatile basic nitrogen and pH during storage, while irradiation increased the levels of thiobarbituric acid reactive substances and protein carbonyls in beef loins.
32750471	4	38	theme	bacteria	1030:1037	arg1	growth					998:1003	the growth	994:1003	the growth of inoculated pathogenic bacteria, including Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella typhimurium	994:1125	All treatments except Ch were effective to control the growth of inoculated pathogenic bacteria, including Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella typhimurium, in loin samples.
32750471	5	39	theme	Ch + CNE + GI	1164:1176	arg1	combination					1149:1159	The combination	1145:1159	The combination of Ch + CNE + GI	1145:1176	The combination of Ch + CNE + GI was the most effective treatment to control the population of microbial flora and inoculated pathogens, slow down some physicochemical changes, and enhance the storage life of beef loins.
32750471	6	40	theme	chitosan	1405:1412	arg1	film					1414:1417	active chitosan film	1398:1417	active chitosan film	1398:1417	As a result, the combination of active chitosan film and low-dose gamma irradiation can ensure microbiological safety and is suggested for long time preservation of beef during chilled storage.
32750471	0	41	theme	loins	181:185	arg1	quality					165:171	quality	165:171	quality	165:171	Effect of edible composite film based on chitosan and cumin essential oil-loaded nanoemulsion combined with low-dose gamma irradiation on microbiological safety and quality of beef loins during refrigerated storage.
32750471	0	41	theme	loins	181:185	arg1	safety					154:159	microbiological safety	138:159	microbiological safety	138:159	Effect of edible composite film based on chitosan and cumin essential oil-loaded nanoemulsion combined with low-dose gamma irradiation on microbiological safety and quality of beef loins during refrigerated storage.
32750471	4	42	theme	inoculated	1008:1017	arg1	bacteria					1030:1037	inoculated pathogenic bacteria	1008:1037	inoculated pathogenic bacteria	1008:1037	All treatments except Ch were effective to control the growth of inoculated pathogenic bacteria, including Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella typhimurium, in loin samples.
32750471	4	42	theme	inoculated	1008:1017	arg1	typhimurium					1115:1125	Salmonella typhimurium	1104:1125	Salmonella typhimurium	1104:1125	All treatments except Ch were effective to control the growth of inoculated pathogenic bacteria, including Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella typhimurium, in loin samples.
32750471	4	42	theme	inoculated	1008:1017	arg1	monocytogenes					1059:1071	Listeria monocytogenes	1050:1071	Listeria monocytogenes	1050:1071	All treatments except Ch were effective to control the growth of inoculated pathogenic bacteria, including Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella typhimurium, in loin samples.
32750471	4	42	theme	inoculated	1008:1017	arg1	O157					1091:1094	O157	1091:1094	Escherichia coli O157:H7	1074:1097	All treatments except Ch were effective to control the growth of inoculated pathogenic bacteria, including Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella typhimurium, in loin samples.
32750471	1	43	theme	loins	439:443	arg1	quality					423:429	microbiological safety and quality	396:429	quality	423:429	This research was conducted to assess the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins during 21 days of chilled storage.
32750471	1	43	theme	loins	439:443	arg1	safety					412:417	microbiological safety and quality	396:429	safety	412:417	This research was conducted to assess the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins during 21 days of chilled storage.
32750471	0	44	theme	refrigerated	194:205	arg1	storage					207:213	refrigerated storage	194:213	refrigerated storage	194:213	Effect of edible composite film based on chitosan and cumin essential oil-loaded nanoemulsion combined with low-dose gamma irradiation on microbiological safety and quality of beef loins during refrigerated storage.
32750471	2	45	theme	acid	564:567	arg1	bacteria					569:576	lactic acid bacteria	557:576	lactic acid bacteria	557:576	The growth of mesophilic and psychrophilic bacteria, Enterobacteriaceae, and lactic acid bacteria were retarded in all treated groups (Ch, GI, Ch + CNE, Ch + GI, and Ch + CNE + GI groups) compared to control group during storage time.
32750471	2	46	theme	treated	599:605	arg1	groups					607:612	all treated groups	595:612	all treated groups	595:612	The growth of mesophilic and psychrophilic bacteria, Enterobacteriaceae, and lactic acid bacteria were retarded in all treated groups (Ch, GI, Ch + CNE, Ch + GI, and Ch + CNE + GI groups) compared to control group during storage time.
32750471	5	47	theme	beef	1354:1357	arg1	loins					1359:1363	beef loins	1354:1363	beef loins	1354:1363	The combination of Ch + CNE + GI was the most effective treatment to control the population of microbial flora and inoculated pathogens, slow down some physicochemical changes, and enhance the storage life of beef loins.
32750471	6	48	theme	microbiological	1461:1475	arg1	safety					1477:1482	microbiological safety	1461:1482	microbiological safety	1461:1482	As a result, the combination of active chitosan film and low-dose gamma irradiation can ensure microbiological safety and is suggested for long time preservation of beef during chilled storage.
32750471	6	48	theme	microbiological	1461:1475	arg1	result					1371:1376	a result	1369:1376	a result	1369:1376	As a result, the combination of active chitosan film and low-dose gamma irradiation can ensure microbiological safety and is suggested for long time preservation of beef during chilled storage.
32750471	1	49	from	effect	267:272	arg1	quality					423:429	microbiological safety and quality	396:429	quality	423:429	This research was conducted to assess the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins during 21 days of chilled storage.
32750471	1	49	from	effect	267:272	arg1	safety					412:417	microbiological safety and quality	396:429	safety	412:417	This research was conducted to assess the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins during 21 days of chilled storage.
32750471	1	50	contain	containing	296:305	arg2	irradiation					365:375	low-dose gamma irradiation	350:375	low-dose gamma irradiation (GI)	350:380	This research was conducted to assess the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins during 21 days of chilled storage.
32750471	1	50	contain	containing	296:305	arg2	CNE					341:343	CNE	341:343	CNE	341:343	This research was conducted to assess the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins during 21 days of chilled storage.
32750471	1	50	contain	containing	296:305	arg2	nanoemulsion					327:338	cumin essential oil nanoemulsion	307:338	cumin essential oil nanoemulsion (CNE)	307:344	This research was conducted to assess the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins during 21 days of chilled storage.
32750471	1	50	contain	containing	296:305	arg1	film					291:294	chitosan (Ch) film	277:294	chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy	277:391	This research was conducted to assess the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins during 21 days of chilled storage.
32750471	1	50	contain	containing	296:305	arg2	GI					378:379	GI	378:379	GI	378:379	This research was conducted to assess the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins during 21 days of chilled storage.
32750471	2	51	theme	bacteria	523:530	arg1	growth					484:489	The growth	480:489	The growth of mesophilic and psychrophilic bacteria, Enterobacteriaceae, and lactic acid bacteria	480:576	The growth of mesophilic and psychrophilic bacteria, Enterobacteriaceae, and lactic acid bacteria were retarded in all treated groups (Ch, GI, Ch + CNE, Ch + GI, and Ch + CNE + GI groups) compared to control group during storage time.
32750471	4	52	theme	Escherichia	1074:1084	arg1	O157					1091:1094	O157	1091:1094	Escherichia coli O157:H7	1074:1097	All treatments except Ch were effective to control the growth of inoculated pathogenic bacteria, including Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella typhimurium, in loin samples.
32750471	0	53	theme	microbiological	138:152	arg1	safety					154:159	microbiological safety	138:159	microbiological safety	138:159	Effect of edible composite film based on chitosan and cumin essential oil-loaded nanoemulsion combined with low-dose gamma irradiation on microbiological safety and quality of beef loins during refrigerated storage.
32750471	6	54	theme	gamma	1432:1436	arg1	irradiation					1438:1448	low-dose gamma irradiation	1423:1448	low-dose gamma irradiation	1423:1448	As a result, the combination of active chitosan film and low-dose gamma irradiation can ensure microbiological safety and is suggested for long time preservation of beef during chilled storage.
32750471	6	55	theme	beef	1531:1534	arg1	preservation					1515:1526	long time preservation	1505:1526	long time preservation of beef during chilled storage	1505:1557	As a result, the combination of active chitosan film and low-dose gamma irradiation can ensure microbiological safety and is suggested for long time preservation of beef during chilled storage.
32750471	3	56	theme	pH	805:806	arg1	level					762:766	the increasing level	747:766	the increasing level of total volatile basic nitrogen and pH	747:806	The treatments also slowed down the increasing level of total volatile basic nitrogen and pH during storage, while irradiation increased the levels of thiobarbituric acid reactive substances and protein carbonyls in beef loins.
32750471	2	57	theme	Enterobacteriaceae	533:550	arg1	growth					484:489	The growth	480:489	The growth of mesophilic and psychrophilic bacteria, Enterobacteriaceae, and lactic acid bacteria	480:576	The growth of mesophilic and psychrophilic bacteria, Enterobacteriaceae, and lactic acid bacteria were retarded in all treated groups (Ch, GI, Ch + CNE, Ch + GI, and Ch + CNE + GI groups) compared to control group during storage time.
32750471	2	58	theme	control	680:686	arg1	group					688:692	control group	680:692	control group	680:692	The growth of mesophilic and psychrophilic bacteria, Enterobacteriaceae, and lactic acid bacteria were retarded in all treated groups (Ch, GI, Ch + CNE, Ch + GI, and Ch + CNE + GI groups) compared to control group during storage time.
32750471	3	59	theme	increasing	751:760	arg1	level					762:766	the increasing level	747:766	the increasing level of total volatile basic nitrogen and pH	747:806	The treatments also slowed down the increasing level of total volatile basic nitrogen and pH during storage, while irradiation increased the levels of thiobarbituric acid reactive substances and protein carbonyls in beef loins.
32750471	0	60	theme	cumin	54:58	arg1	nanoemulsion					81:92	cumin essential oil-loaded nanoemulsion	54:92	cumin essential oil-loaded nanoemulsion	54:92	Effect of edible composite film based on chitosan and cumin essential oil-loaded nanoemulsion combined with low-dose gamma irradiation on microbiological safety and quality of beef loins during refrigerated storage.
32750471	1	61	theme	microbiological	396:410	arg1	safety					412:417	microbiological safety and quality	396:429	safety	412:417	This research was conducted to assess the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins during 21 days of chilled storage.
32750471	6	62	theme	long	1505:1508	arg1	preservation					1515:1526	long time preservation	1505:1526	long time preservation of beef during chilled storage	1505:1557	As a result, the combination of active chitosan film and low-dose gamma irradiation can ensure microbiological safety and is suggested for long time preservation of beef during chilled storage.
32750471	3	63	theme	nitrogen	792:799	arg1	level					762:766	the increasing level	747:766	the increasing level of total volatile basic nitrogen and pH	747:806	The treatments also slowed down the increasing level of total volatile basic nitrogen and pH during storage, while irradiation increased the levels of thiobarbituric acid reactive substances and protein carbonyls in beef loins.
32750471	3	64	from	levels	856:861	arg1	loins					936:940	beef loins	931:940	beef loins	931:940	The treatments also slowed down the increasing level of total volatile basic nitrogen and pH during storage, while irradiation increased the levels of thiobarbituric acid reactive substances and protein carbonyls in beef loins.
32750471	5	65	theme	effective	1191:1199	arg1	treatment					1201:1209	the most effective treatment	1182:1209	the most effective treatment	1182:1209	The combination of Ch + CNE + GI was the most effective treatment to control the population of microbial flora and inoculated pathogens, slow down some physicochemical changes, and enhance the storage life of beef loins.
32750471	0	66	theme	oil-loaded	70:79	arg1	nanoemulsion					81:92	cumin essential oil-loaded nanoemulsion	54:92	cumin essential oil-loaded nanoemulsion	54:92	Effect of edible composite film based on chitosan and cumin essential oil-loaded nanoemulsion combined with low-dose gamma irradiation on microbiological safety and quality of beef loins during refrigerated storage.
32750471	3	67	theme	volatile	777:784	arg1	nitrogen					792:799	total volatile basic nitrogen	771:799	total volatile basic nitrogen	771:799	The treatments also slowed down the increasing level of total volatile basic nitrogen and pH during storage, while irradiation increased the levels of thiobarbituric acid reactive substances and protein carbonyls in beef loins.
32750471	0	68	theme	low-dose	108:115	arg1	irradiation					123:133	low-dose gamma irradiation	108:133	low-dose gamma irradiation	108:133	Effect of edible composite film based on chitosan and cumin essential oil-loaded nanoemulsion combined with low-dose gamma irradiation on microbiological safety and quality of beef loins during refrigerated storage.
32750471	1	69	theme	gamma	359:363	arg1	GI					378:379	GI	378:379	GI	378:379	This research was conducted to assess the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins during 21 days of chilled storage.
32750471	1	69	theme	gamma	359:363	arg1	irradiation					365:375	low-dose gamma irradiation	350:375	low-dose gamma irradiation (GI)	350:380	This research was conducted to assess the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins during 21 days of chilled storage.
32750471	3	70	from	loins	936:940	arg1	levels					856:861	the levels	852:861	the levels of thiobarbituric acid reactive substances and protein carbonyls in beef loins	852:940	The treatments also slowed down the increasing level of total volatile basic nitrogen and pH during storage, while irradiation increased the levels of thiobarbituric acid reactive substances and protein carbonyls in beef loins.
32750471	5	71	theme	flora	1250:1254	arg1	population					1226:1235	the population	1222:1235	the population of microbial flora and inoculated pathogens, slow down some physicochemical changes,	1222:1320	The combination of Ch + CNE + GI was the most effective treatment to control the population of microbial flora and inoculated pathogens, slow down some physicochemical changes, and enhance the storage life of beef loins.
32750471	0	72	theme	composite	17:25	arg1	film					27:30	edible composite film	10:30	edible composite film	10:30	Effect of edible composite film based on chitosan and cumin essential oil-loaded nanoemulsion combined with low-dose gamma irradiation on microbiological safety and quality of beef loins during refrigerated storage.
32750471	6	73	theme	chilled	1543:1549	arg1	storage					1551:1557	chilled storage	1543:1557	chilled storage	1543:1557	As a result, the combination of active chitosan film and low-dose gamma irradiation can ensure microbiological safety and is suggested for long time preservation of beef during chilled storage.
32750471	3	74	theme	reactive	886:893	arg1	substances					895:904	thiobarbituric acid reactive substances	866:904	thiobarbituric acid reactive substances	866:904	The treatments also slowed down the increasing level of total volatile basic nitrogen and pH during storage, while irradiation increased the levels of thiobarbituric acid reactive substances and protein carbonyls in beef loins.
32750471	3	75	from	substances	895:904	arg1	loins					936:940	beef loins	931:940	beef loins	931:940	The treatments also slowed down the increasing level of total volatile basic nitrogen and pH during storage, while irradiation increased the levels of thiobarbituric acid reactive substances and protein carbonyls in beef loins.
32750471	5	76	theme	inoculated	1260:1269	arg1	pathogens					1271:1279	inoculated pathogens	1260:1279	inoculated pathogens	1260:1279	The combination of Ch + CNE + GI was the most effective treatment to control the population of microbial flora and inoculated pathogens, slow down some physicochemical changes, and enhance the storage life of beef loins.
32750471	4	77	from	samples	1136:1142	arg1	effective					973:981	effective	973:981	effective	973:981	All treatments except Ch were effective to control the growth of inoculated pathogenic bacteria, including Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella typhimurium, in loin samples.
32750471	3	78	theme	thiobarbituric	866:879	arg1	substances					895:904	thiobarbituric acid reactive substances	866:904	thiobarbituric acid reactive substances	866:904	The treatments also slowed down the increasing level of total volatile basic nitrogen and pH during storage, while irradiation increased the levels of thiobarbituric acid reactive substances and protein carbonyls in beef loins.
32750471	3	79	theme	carbonyls	918:926	arg1	levels					856:861	the levels	852:861	the levels of thiobarbituric acid reactive substances and protein carbonyls in beef loins	852:940	The treatments also slowed down the increasing level of total volatile basic nitrogen and pH during storage, while irradiation increased the levels of thiobarbituric acid reactive substances and protein carbonyls in beef loins.
32750471	1	80	theme	storage	471:477	arg1	21 days					452:458	21 days	452:458	21 days of chilled storage	452:477	This research was conducted to assess the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins during 21 days of chilled storage.
32750471	6	81	theme	low-dose	1423:1430	arg1	irradiation					1438:1448	low-dose gamma irradiation	1423:1448	low-dose gamma irradiation	1423:1448	As a result, the combination of active chitosan film and low-dose gamma irradiation can ensure microbiological safety and is suggested for long time preservation of beef during chilled storage.
32750471	1	82	theme	essential	313:321	arg1	CNE					341:343	CNE	341:343	CNE	341:343	This research was conducted to assess the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins during 21 days of chilled storage.
32750471	1	82	theme	essential	313:321	arg1	nanoemulsion					327:338	cumin essential oil nanoemulsion	307:338	cumin essential oil nanoemulsion (CNE)	307:344	This research was conducted to assess the combined effect of chitosan (Ch) film containing cumin essential oil nanoemulsion (CNE) and low-dose gamma irradiation (GI) at 2.5 kGy on microbiological safety and quality of beef loins during 21 days of chilled storage.
32750471	5	83	theme	storage	1338:1344	arg1	life					1346:1349	the storage life	1334:1349	the storage life of beef loins	1334:1363	The combination of Ch + CNE + GI was the most effective treatment to control the population of microbial flora and inoculated pathogens, slow down some physicochemical changes, and enhance the storage life of beef loins.
32750471	4	84	theme	pathogenic	1019:1028	arg1	bacteria					1030:1037	inoculated pathogenic bacteria	1008:1037	inoculated pathogenic bacteria	1008:1037	All treatments except Ch were effective to control the growth of inoculated pathogenic bacteria, including Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella typhimurium, in loin samples.
32750471	4	84	theme	pathogenic	1019:1028	arg1	typhimurium					1115:1125	Salmonella typhimurium	1104:1125	Salmonella typhimurium	1104:1125	All treatments except Ch were effective to control the growth of inoculated pathogenic bacteria, including Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella typhimurium, in loin samples.
32750471	4	84	theme	pathogenic	1019:1028	arg1	monocytogenes					1059:1071	Listeria monocytogenes	1050:1071	Listeria monocytogenes	1050:1071	All treatments except Ch were effective to control the growth of inoculated pathogenic bacteria, including Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella typhimurium, in loin samples.
32750471	4	84	theme	pathogenic	1019:1028	arg1	O157					1091:1094	O157	1091:1094	Escherichia coli O157:H7	1074:1097	All treatments except Ch were effective to control the growth of inoculated pathogenic bacteria, including Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella typhimurium, in loin samples.
32750471	5	85	theme	physicochemical	1297:1311	arg1	flora					1250:1254	microbial flora	1240:1254	microbial flora	1240:1254	The combination of Ch + CNE + GI was the most effective treatment to control the population of microbial flora and inoculated pathogens, slow down some physicochemical changes, and enhance the storage life of beef loins.
32750471	5	85	theme	physicochemical	1297:1311	arg1	changes					1313:1319	some physicochemical changes	1292:1319	slow down some physicochemical changes	1282:1319	The combination of Ch + CNE + GI was the most effective treatment to control the population of microbial flora and inoculated pathogens, slow down some physicochemical changes, and enhance the storage life of beef loins.
32750471	6	86	theme	film	1414:1417	arg1	combination					1383:1393	the combination	1379:1393	the combination of active chitosan film and low-dose gamma irradiation	1379:1448	As a result, the combination of active chitosan film and low-dose gamma irradiation can ensure microbiological safety and is suggested for long time preservation of beef during chilled storage.
32750471	6	87	theme	active	1398:1403	arg1	film					1414:1417	active chitosan film	1398:1417	active chitosan film	1398:1417	As a result, the combination of active chitosan film and low-dose gamma irradiation can ensure microbiological safety and is suggested for long time preservation of beef during chilled storage.
32127446	7	0	theme	other	1432:1436	arg1	species					1438:1444	other species	1432:1444	other species	1432:1444	While mucins in C. elegans lack sialic acid caps, which makes their monosaccharides readily available, they are capped in other species.
32127446	4	1	theme	derived	551:557	arg1	glycans					559:565	derived glycans	551:565	derived glycans	551:565	It has also been recently reported that individual mucins and derived glycans can attenuate the virulence of the human pathogen Pseudomonas aeruginosa Here, we show data indicating that mucins not only play a role in host defense but that they can also be subverted by P. aeruginosa to cause disease.
32127446	6	2	theme	organisms	1232:1240	arg1	range					1223:1227	a wide range	1216:1227	a wide range of organisms where they act as host factors required for bacterial pathogenesis	1216:1307	The monosaccharides identified in this study are found in a wide range of organisms where they act as host factors required for bacterial pathogenesis.
32127446	10	3	attach	derived	1972:1978	arg2	monosaccharides					1948:1962	host monosaccharides	1943:1962	host monosaccharides	1943:1962	In this study, we describe a requirement of host monosaccharides, likely derived from host mucins, for the ability of Pseudomonas aeruginosa to colonize the intestine and ultimately cause death in Caenorhabditis elegans We also demonstrate that monosaccharides alter the ability of bacteria to bind to both Caenorhabditis elegans intestinal cells and human lung alveolar epithelial cells, suggesting that there are conserved mechanisms underlying host-pathogen interactions in a range of organisms.
32127446	10	3	attach	derived	1972:1978	arg1	mucins					1990:1995	host mucins	1985:1995	host mucins	1985:1995	In this study, we describe a requirement of host monosaccharides, likely derived from host mucins, for the ability of Pseudomonas aeruginosa to colonize the intestine and ultimately cause death in Caenorhabditis elegans We also demonstrate that monosaccharides alter the ability of bacteria to bind to both Caenorhabditis elegans intestinal cells and human lung alveolar epithelial cells, suggesting that there are conserved mechanisms underlying host-pathogen interactions in a range of organisms.
32127446	8	4	theme	epithelial	1645:1654	arg1	tissues					1656:1662	mucosal epithelial tissues	1637:1662	mucosal epithelial tissues	1637:1662	Pathogens such as P. aeruginosa that lack sialidases may rely on enzymes from other bacteria to utilize mucin-derived monosaccharides.IMPORTANCE One of the first lines of defense present at mucosal epithelial tissues is mucus, which is a highly viscous material formed by mucin glycoproteins.
32127446	10	5	theme	human	2250:2254	arg1	cells					2281:2285	human lung alveolar epithelial cells	2250:2285	human lung alveolar epithelial cells	2250:2285	In this study, we describe a requirement of host monosaccharides, likely derived from host mucins, for the ability of Pseudomonas aeruginosa to colonize the intestine and ultimately cause death in Caenorhabditis elegans We also demonstrate that monosaccharides alter the ability of bacteria to bind to both Caenorhabditis elegans intestinal cells and human lung alveolar epithelial cells, suggesting that there are conserved mechanisms underlying host-pathogen interactions in a range of organisms.
32127446	10	6	theme	organisms	2387:2395	arg1	range					2378:2382	a range	2376:2382	a range of organisms	2376:2395	In this study, we describe a requirement of host monosaccharides, likely derived from host mucins, for the ability of Pseudomonas aeruginosa to colonize the intestine and ultimately cause death in Caenorhabditis elegans We also demonstrate that monosaccharides alter the ability of bacteria to bind to both Caenorhabditis elegans intestinal cells and human lung alveolar epithelial cells, suggesting that there are conserved mechanisms underlying host-pathogen interactions in a range of organisms.
32127446	10	7	theme	aeruginosa	2029:2038	arg1	ability					2006:2012	the ability	2002:2012	the ability of Pseudomonas aeruginosa to colonize the intestine and ultimately cause death in Caenorhabditis elegans We also demonstrate that monosaccharides alter the ability of bacteria to bind to both Caenorhabditis elegans intestinal cells and human lung alveolar epithelial cells, suggesting that there are conserved mechanisms underlying host-pathogen interactions in a range of organisms	2002:2395	In this study, we describe a requirement of host monosaccharides, likely derived from host mucins, for the ability of Pseudomonas aeruginosa to colonize the intestine and ultimately cause death in Caenorhabditis elegans We also demonstrate that monosaccharides alter the ability of bacteria to bind to both Caenorhabditis elegans intestinal cells and human lung alveolar epithelial cells, suggesting that there are conserved mechanisms underlying host-pathogen interactions in a range of organisms.
32127446	1	8	theme	lining	173:178	arg1	cells					191:195	lining epithelial cells	173:195	lining epithelial cells	173:195	One of the primary functions of the mucosal barrier, found lining epithelial cells, is to serve as a first-line of defense against microbial pathogens.
32127446	7	9	theme	sialic	1342:1347	arg1	caps					1354:1357	sialic acid caps	1342:1357	sialic acid caps	1342:1357	While mucins in C. elegans lack sialic acid caps, which makes their monosaccharides readily available, they are capped in other species.
32127446	9	10	theme	innate	1876:1881	arg1	they					1788:1791	they	1788:1791	they	1788:1791	Mucins serve various functions, but importantly they aid in the clearance of pathogens and debris from epithelial barriers and serve as innate immune factors.
32127446	9	10	theme	innate	1876:1881	arg1	factors					1890:1896	innate immune factors	1876:1896	innate immune factors	1876:1896	Mucins serve various functions, but importantly they aid in the clearance of pathogens and debris from epithelial barriers and serve as innate immune factors.
32127446	4	11	theme	human	602:606	arg1	pathogen					608:615	the human pathogen	598:615	the human pathogen Pseudomonas aeruginosa Here, we show data indicating that mucins not only play a role in host defense but that they can also be subverted by P. aeruginosa to cause disease	598:787	It has also been recently reported that individual mucins and derived glycans can attenuate the virulence of the human pathogen Pseudomonas aeruginosa Here, we show data indicating that mucins not only play a role in host defense but that they can also be subverted by P. aeruginosa to cause disease.
32127446	5	12	theme	lung	1034:1037	arg1	cells					1059:1063	human lung alveolar epithelial cells	1028:1063	human lung alveolar epithelial cells	1028:1063	We found that the mucin MUL-1 and mucin-derived monosaccharides N-acetyl-galactosamine and N-acetylglucosamine are required for P. aeruginosa killing of Caenorhabditis elegans We also found that the defective adhesion of P. aeruginosa to human lung alveolar epithelial cells, deficient in the mucin MUC1, can be reversed by the addition of individual monosaccharides.
32127446	2	13	theme	major	270:274	arg1	components					287:296	The major structural components	266:296	The major structural components of mucus	266:305	The major structural components of mucus are heavily glycosylated proteins called mucins.
32127446	2	13	theme	major	270:274	arg1	proteins					332:339	heavily glycosylated proteins	311:339	heavily glycosylated proteins called mucins	311:353	The major structural components of mucus are heavily glycosylated proteins called mucins.
32127446	10	14	theme	alveolar	2261:2268	arg1	cells					2281:2285	human lung alveolar epithelial cells	2250:2285	human lung alveolar epithelial cells	2250:2285	In this study, we describe a requirement of host monosaccharides, likely derived from host mucins, for the ability of Pseudomonas aeruginosa to colonize the intestine and ultimately cause death in Caenorhabditis elegans We also demonstrate that monosaccharides alter the ability of bacteria to bind to both Caenorhabditis elegans intestinal cells and human lung alveolar epithelial cells, suggesting that there are conserved mechanisms underlying host-pathogen interactions in a range of organisms.
32127446	8	15	link	mucin-derived	1551:1563	arg1	monosaccharides.IMPORTANCE					1565:1590	mucin-derived monosaccharides.IMPORTANCE	1551:1590	mucin-derived monosaccharides.IMPORTANCE	1551:1590	Pathogens such as P. aeruginosa that lack sialidases may rely on enzymes from other bacteria to utilize mucin-derived monosaccharides.IMPORTANCE One of the first lines of defense present at mucosal epithelial tissues is mucus, which is a highly viscous material formed by mucin glycoproteins.
32127446	8	16	from	bacteria	1531:1538	arg1	enzymes					1512:1518	enzymes	1512:1518	enzymes from other bacteria	1512:1538	Pathogens such as P. aeruginosa that lack sialidases may rely on enzymes from other bacteria to utilize mucin-derived monosaccharides.IMPORTANCE One of the first lines of defense present at mucosal epithelial tissues is mucus, which is a highly viscous material formed by mucin glycoproteins.
32127446	5	17	theme	epithelial	1048:1057	arg1	cells					1059:1063	human lung alveolar epithelial cells	1028:1063	human lung alveolar epithelial cells	1028:1063	We found that the mucin MUL-1 and mucin-derived monosaccharides N-acetyl-galactosamine and N-acetylglucosamine are required for P. aeruginosa killing of Caenorhabditis elegans We also found that the defective adhesion of P. aeruginosa to human lung alveolar epithelial cells, deficient in the mucin MUC1, can be reversed by the addition of individual monosaccharides.
32127446	10	18	theme	Caenorhabditis	2096:2109	arg1	elegans					2111:2117	Caenorhabditis elegans	2096:2117	Caenorhabditis elegans	2096:2117	In this study, we describe a requirement of host monosaccharides, likely derived from host mucins, for the ability of Pseudomonas aeruginosa to colonize the intestine and ultimately cause death in Caenorhabditis elegans We also demonstrate that monosaccharides alter the ability of bacteria to bind to both Caenorhabditis elegans intestinal cells and human lung alveolar epithelial cells, suggesting that there are conserved mechanisms underlying host-pathogen interactions in a range of organisms.
32127446	4	19	theme	host	706:709	arg1	defense					711:717	host defense	706:717	host defense	706:717	It has also been recently reported that individual mucins and derived glycans can attenuate the virulence of the human pathogen Pseudomonas aeruginosa Here, we show data indicating that mucins not only play a role in host defense but that they can also be subverted by P. aeruginosa to cause disease.
32127446	10	20	theme	monosaccharides	1948:1962	arg1	requirement					1928:1938	a requirement	1926:1938	a requirement of host monosaccharides, likely derived from host mucins, for the ability of Pseudomonas aeruginosa to colonize the intestine and ultimately cause death in Caenorhabditis elegans We also demonstrate that monosaccharides alter the ability of bacteria to bind to both Caenorhabditis elegans intestinal cells and human lung alveolar epithelial cells, suggesting that there are conserved mechanisms underlying host-pathogen interactions in a range of organisms	1926:2395	In this study, we describe a requirement of host monosaccharides, likely derived from host mucins, for the ability of Pseudomonas aeruginosa to colonize the intestine and ultimately cause death in Caenorhabditis elegans We also demonstrate that monosaccharides alter the ability of bacteria to bind to both Caenorhabditis elegans intestinal cells and human lung alveolar epithelial cells, suggesting that there are conserved mechanisms underlying host-pathogen interactions in a range of organisms.
32127446	1	21	theme	primary	125:131	arg1	functions					133:141	the primary functions	121:141	the primary functions of the mucosal barrier	121:164	One of the primary functions of the mucosal barrier, found lining epithelial cells, is to serve as a first-line of defense against microbial pathogens.
32127446	11	22	theme	pathogen	2516:2523	arg1	infection					2525:2533	pathogen infection	2516:2533	pathogen infection	2516:2533	By gaining a better understanding of pathogen-mucin interactions, we can develop better approaches to protect against pathogen infection.
32127446	11	23	theme	better	2411:2416	arg1	understanding					2418:2430	a better understanding	2409:2430	a better understanding of pathogen-mucin interactions	2409:2461	By gaining a better understanding of pathogen-mucin interactions, we can develop better approaches to protect against pathogen infection.
32127446	5	24	dep	P.	918:919	arg1	aeruginosa					921:930	aeruginosa	921:930	aeruginosa	921:930	We found that the mucin MUL-1 and mucin-derived monosaccharides N-acetyl-galactosamine and N-acetylglucosamine are required for P. aeruginosa killing of Caenorhabditis elegans We also found that the defective adhesion of P. aeruginosa to human lung alveolar epithelial cells, deficient in the mucin MUC1, can be reversed by the addition of individual monosaccharides.
32127446	5	25	theme	mucin	1083:1087	arg1	MUC1					1089:1092	the mucin MUC1	1079:1092	the mucin MUC1	1079:1092	We found that the mucin MUL-1 and mucin-derived monosaccharides N-acetyl-galactosamine and N-acetylglucosamine are required for P. aeruginosa killing of Caenorhabditis elegans We also found that the defective adhesion of P. aeruginosa to human lung alveolar epithelial cells, deficient in the mucin MUC1, can be reversed by the addition of individual monosaccharides.
32127446	11	26	theme	interactions	2450:2461	arg1	understanding					2418:2430	a better understanding	2409:2430	a better understanding of pathogen-mucin interactions	2409:2461	By gaining a better understanding of pathogen-mucin interactions, we can develop better approaches to protect against pathogen infection.
32127446	5	27	dep	required	905:912	arg1	found					974:978	found	974:978	found that the defective adhesion of P. aeruginosa to human lung alveolar epithelial cells, deficient in the mucin MUC1, can be reversed by the addition of individual monosaccharides	974:1155	We found that the mucin MUL-1 and mucin-derived monosaccharides N-acetyl-galactosamine and N-acetylglucosamine are required for P. aeruginosa killing of Caenorhabditis elegans We also found that the defective adhesion of P. aeruginosa to human lung alveolar epithelial cells, deficient in the mucin MUC1, can be reversed by the addition of individual monosaccharides.
32127446	1	28	theme	mucosal	150:156	arg1	barrier					158:164	the mucosal barrier	146:164	the mucosal barrier	146:164	One of the primary functions of the mucosal barrier, found lining epithelial cells, is to serve as a first-line of defense against microbial pathogens.
32127446	5	29	theme	elegans	958:964	arg1	killing					932:938	P. aeruginosa killing	918:938	P. aeruginosa killing of Caenorhabditis elegans	918:964	We found that the mucin MUL-1 and mucin-derived monosaccharides N-acetyl-galactosamine and N-acetylglucosamine are required for P. aeruginosa killing of Caenorhabditis elegans We also found that the defective adhesion of P. aeruginosa to human lung alveolar epithelial cells, deficient in the mucin MUC1, can be reversed by the addition of individual monosaccharides.
32127446	6	30	located	found	1207:1211	arg1	range					1223:1227	a wide range	1216:1227	a wide range of organisms where they act as host factors required for bacterial pathogenesis	1216:1307	The monosaccharides identified in this study are found in a wide range of organisms where they act as host factors required for bacterial pathogenesis.
32127446	6	30	located	found	1207:1211	arg2	monosaccharides					1162:1176	The monosaccharides	1158:1176	The monosaccharides identified in this study	1158:1201	The monosaccharides identified in this study are found in a wide range of organisms where they act as host factors required for bacterial pathogenesis.
32127446	3	31	theme	key	367:369	arg1	Mucins					356:361	Mucins	356:361	Mucins	356:361	Mucins are key components of the innate immune system as they aid in the clearance of pathogens and can decrease pathogen virulence.
32127446	3	31	theme	key	367:369	arg1	components					371:380	key components	367:380	key components of the innate immune system	367:408	Mucins are key components of the innate immune system as they aid in the clearance of pathogens and can decrease pathogen virulence.
32127446	3	32	theme	system	403:408	arg1	Mucins					356:361	Mucins	356:361	Mucins	356:361	Mucins are key components of the innate immune system as they aid in the clearance of pathogens and can decrease pathogen virulence.
32127446	3	32	theme	system	403:408	arg1	components					371:380	key components	367:380	key components of the innate immune system	367:408	Mucins are key components of the innate immune system as they aid in the clearance of pathogens and can decrease pathogen virulence.
32127446	0	33	theme	Host	0:3	arg1	Mucin					5:9	Host Mucin	0:9	Host Mucin	0:9	Host Mucin Is Exploited by Pseudomonas aeruginosa To Provide Monosaccharides Required for a Successful Infection.
32127446	1	34	theme	microbial	245:253	arg1	pathogens					255:263	microbial pathogens	245:263	microbial pathogens	245:263	One of the primary functions of the mucosal barrier, found lining epithelial cells, is to serve as a first-line of defense against microbial pathogens.
32127446	8	35	theme	lack	1484:1487	arg1	sialidases					1489:1498	lack sialidases	1484:1498	lack sialidases	1484:1498	Pathogens such as P. aeruginosa that lack sialidases may rely on enzymes from other bacteria to utilize mucin-derived monosaccharides.IMPORTANCE One of the first lines of defense present at mucosal epithelial tissues is mucus, which is a highly viscous material formed by mucin glycoproteins.
32127446	5	36	dep	MUL-1	814:818	arg1	N-acetylglucosamine					881:899	N-acetylglucosamine	881:899	N-acetylglucosamine	881:899	We found that the mucin MUL-1 and mucin-derived monosaccharides N-acetyl-galactosamine and N-acetylglucosamine are required for P. aeruginosa killing of Caenorhabditis elegans We also found that the defective adhesion of P. aeruginosa to human lung alveolar epithelial cells, deficient in the mucin MUC1, can be reversed by the addition of individual monosaccharides.
32127446	5	36	dep	MUL-1	814:818	arg1	N-acetyl-galactosamine					854:875	N-acetyl-galactosamine	854:875	N-acetyl-galactosamine	854:875	We found that the mucin MUL-1 and mucin-derived monosaccharides N-acetyl-galactosamine and N-acetylglucosamine are required for P. aeruginosa killing of Caenorhabditis elegans We also found that the defective adhesion of P. aeruginosa to human lung alveolar epithelial cells, deficient in the mucin MUC1, can be reversed by the addition of individual monosaccharides.
32127446	5	37	from	deficient	1066:1074	arg1	MUC1					1089:1092	the mucin MUC1	1079:1092	the mucin MUC1	1079:1092	We found that the mucin MUL-1 and mucin-derived monosaccharides N-acetyl-galactosamine and N-acetylglucosamine are required for P. aeruginosa killing of Caenorhabditis elegans We also found that the defective adhesion of P. aeruginosa to human lung alveolar epithelial cells, deficient in the mucin MUC1, can be reversed by the addition of individual monosaccharides.
32127446	5	38	theme	individual	1130:1139	arg1	monosaccharides					1141:1155	individual monosaccharides	1130:1155	individual monosaccharides	1130:1155	We found that the mucin MUL-1 and mucin-derived monosaccharides N-acetyl-galactosamine and N-acetylglucosamine are required for P. aeruginosa killing of Caenorhabditis elegans We also found that the defective adhesion of P. aeruginosa to human lung alveolar epithelial cells, deficient in the mucin MUC1, can be reversed by the addition of individual monosaccharides.
32127446	4	39	dep	mucins	675:680	arg1	only					686:689	only	686:689	only	686:689	It has also been recently reported that individual mucins and derived glycans can attenuate the virulence of the human pathogen Pseudomonas aeruginosa Here, we show data indicating that mucins not only play a role in host defense but that they can also be subverted by P. aeruginosa to cause disease.
32127446	3	40	theme	innate	389:394	arg1	system					403:408	the innate immune system	385:408	the innate immune system	385:408	Mucins are key components of the innate immune system as they aid in the clearance of pathogens and can decrease pathogen virulence.
32127446	4	41	theme	individual	529:538	arg1	mucins					540:545	individual mucins	529:545	individual mucins	529:545	It has also been recently reported that individual mucins and derived glycans can attenuate the virulence of the human pathogen Pseudomonas aeruginosa Here, we show data indicating that mucins not only play a role in host defense but that they can also be subverted by P. aeruginosa to cause disease.
32127446	4	42	dep	pathogen	608:615	arg1	show					649:652	show	649:652	show data indicating that mucins not only play a role in host defense but that they can also be subverted by P. aeruginosa to cause disease	649:787	It has also been recently reported that individual mucins and derived glycans can attenuate the virulence of the human pathogen Pseudomonas aeruginosa Here, we show data indicating that mucins not only play a role in host defense but that they can also be subverted by P. aeruginosa to cause disease.
32127446	4	42	dep	pathogen	608:615	arg1	aeruginosa					629:638	Pseudomonas aeruginosa	617:638	the human pathogen Pseudomonas aeruginosa Here, we show data indicating that mucins not only play a role in host defense but that they can also be subverted by P. aeruginosa to cause disease	598:787	It has also been recently reported that individual mucins and derived glycans can attenuate the virulence of the human pathogen Pseudomonas aeruginosa Here, we show data indicating that mucins not only play a role in host defense but that they can also be subverted by P. aeruginosa to cause disease.
32127446	8	43	dep	utilize	1543:1549	arg1	mucus					1667:1671	mucus	1667:1671	mucus	1667:1671	Pathogens such as P. aeruginosa that lack sialidases may rely on enzymes from other bacteria to utilize mucin-derived monosaccharides.IMPORTANCE One of the first lines of defense present at mucosal epithelial tissues is mucus, which is a highly viscous material formed by mucin glycoproteins.
32127446	8	43	dep	utilize	1543:1549	arg1	material					1700:1707	a highly viscous material	1683:1707	a highly viscous material formed by mucin glycoproteins	1683:1737	Pathogens such as P. aeruginosa that lack sialidases may rely on enzymes from other bacteria to utilize mucin-derived monosaccharides.IMPORTANCE One of the first lines of defense present at mucosal epithelial tissues is mucus, which is a highly viscous material formed by mucin glycoproteins.
32127446	2	44	theme	glycosylated	319:330	arg1	components					287:296	The major structural components	266:296	The major structural components of mucus	266:305	The major structural components of mucus are heavily glycosylated proteins called mucins.
32127446	2	44	theme	glycosylated	319:330	arg1	proteins					332:339	heavily glycosylated proteins	311:339	heavily glycosylated proteins called mucins	311:353	The major structural components of mucus are heavily glycosylated proteins called mucins.
32127446	5	45	from	MUC1	1089:1092	arg1	deficient					1066:1074	deficient	1066:1074	deficient	1066:1074	We found that the mucin MUL-1 and mucin-derived monosaccharides N-acetyl-galactosamine and N-acetylglucosamine are required for P. aeruginosa killing of Caenorhabditis elegans We also found that the defective adhesion of P. aeruginosa to human lung alveolar epithelial cells, deficient in the mucin MUC1, can be reversed by the addition of individual monosaccharides.
32127446	5	46	theme	defective	989:997	arg1	adhesion					999:1006	the defective adhesion	985:1006	the defective adhesion of P. aeruginosa to human lung alveolar epithelial cells, deficient in the mucin MUC1,	985:1093	We found that the mucin MUL-1 and mucin-derived monosaccharides N-acetyl-galactosamine and N-acetylglucosamine are required for P. aeruginosa killing of Caenorhabditis elegans We also found that the defective adhesion of P. aeruginosa to human lung alveolar epithelial cells, deficient in the mucin MUC1, can be reversed by the addition of individual monosaccharides.
32127446	3	47	theme	pathogens	442:450	arg1	clearance					429:437	the clearance	425:437	the clearance of pathogens	425:450	Mucins are key components of the innate immune system as they aid in the clearance of pathogens and can decrease pathogen virulence.
32127446	8	48	theme	first	1603:1607	arg1	lines					1609:1613	the first lines	1599:1613	the first lines of defense present at mucosal epithelial tissues	1599:1662	Pathogens such as P. aeruginosa that lack sialidases may rely on enzymes from other bacteria to utilize mucin-derived monosaccharides.IMPORTANCE One of the first lines of defense present at mucosal epithelial tissues is mucus, which is a highly viscous material formed by mucin glycoproteins.
32127446	4	49	link	derived	551:557	arg1	glycans					559:565	derived glycans	551:565	derived glycans	551:565	It has also been recently reported that individual mucins and derived glycans can attenuate the virulence of the human pathogen Pseudomonas aeruginosa Here, we show data indicating that mucins not only play a role in host defense but that they can also be subverted by P. aeruginosa to cause disease.
32127446	9	50	theme	pathogens	1817:1825	arg1	clearance					1804:1812	the clearance	1800:1812	the clearance of pathogens and debris from epithelial barriers	1800:1861	Mucins serve various functions, but importantly they aid in the clearance of pathogens and debris from epithelial barriers and serve as innate immune factors.
32127446	3	51	theme	pathogen	469:476	arg1	virulence					478:486	pathogen virulence	469:486	pathogen virulence	469:486	Mucins are key components of the innate immune system as they aid in the clearance of pathogens and can decrease pathogen virulence.
32127446	8	52	theme	present	1626:1632	arg1	defense					1618:1624	defense	1618:1624	defense present at mucosal epithelial tissues	1618:1662	Pathogens such as P. aeruginosa that lack sialidases may rely on enzymes from other bacteria to utilize mucin-derived monosaccharides.IMPORTANCE One of the first lines of defense present at mucosal epithelial tissues is mucus, which is a highly viscous material formed by mucin glycoproteins.
32127446	10	53	theme	Caenorhabditis	2206:2219	arg1	cells					2240:2244	Caenorhabditis elegans intestinal cells	2206:2244	Caenorhabditis elegans intestinal cells	2206:2244	In this study, we describe a requirement of host monosaccharides, likely derived from host mucins, for the ability of Pseudomonas aeruginosa to colonize the intestine and ultimately cause death in Caenorhabditis elegans We also demonstrate that monosaccharides alter the ability of bacteria to bind to both Caenorhabditis elegans intestinal cells and human lung alveolar epithelial cells, suggesting that there are conserved mechanisms underlying host-pathogen interactions in a range of organisms.
32127446	9	54	theme	debris	1831:1836	arg1	clearance					1804:1812	the clearance	1800:1812	the clearance of pathogens and debris from epithelial barriers	1800:1861	Mucins serve various functions, but importantly they aid in the clearance of pathogens and debris from epithelial barriers and serve as innate immune factors.
32127446	8	55	theme	mucosal	1637:1643	arg1	tissues					1656:1662	mucosal epithelial tissues	1637:1662	mucosal epithelial tissues	1637:1662	Pathogens such as P. aeruginosa that lack sialidases may rely on enzymes from other bacteria to utilize mucin-derived monosaccharides.IMPORTANCE One of the first lines of defense present at mucosal epithelial tissues is mucus, which is a highly viscous material formed by mucin glycoproteins.
32127446	1	56	theme	defense	229:235	arg1	One					114:116	One	114:116	One	114:116	One of the primary functions of the mucosal barrier, found lining epithelial cells, is to serve as a first-line of defense against microbial pathogens.
32127446	1	56	theme	defense	229:235	arg1	functions					133:141	the primary functions	121:141	the primary functions of the mucosal barrier	121:164	One of the primary functions of the mucosal barrier, found lining epithelial cells, is to serve as a first-line of defense against microbial pathogens.
32127446	1	56	theme	defense	229:235	arg1	first-line					215:224	a first-line	213:224	a first-line of defense against microbial pathogens	213:263	One of the primary functions of the mucosal barrier, found lining epithelial cells, is to serve as a first-line of defense against microbial pathogens.
32127446	9	57	theme	epithelial	1843:1852	arg1	barriers					1854:1861	epithelial barriers	1843:1861	epithelial barriers	1843:1861	Mucins serve various functions, but importantly they aid in the clearance of pathogens and debris from epithelial barriers and serve as innate immune factors.
32127446	6	58	theme	wide	1218:1221	arg1	range					1223:1227	a wide range	1216:1227	a wide range of organisms where they act as host factors required for bacterial pathogenesis	1216:1307	The monosaccharides identified in this study are found in a wide range of organisms where they act as host factors required for bacterial pathogenesis.
32127446	1	59	theme	barrier	158:164	arg1	functions					133:141	the primary functions	121:141	the primary functions of the mucosal barrier	121:164	One of the primary functions of the mucosal barrier, found lining epithelial cells, is to serve as a first-line of defense against microbial pathogens.
32127446	10	60	from	death	2087:2091	arg1	elegans					2111:2117	Caenorhabditis elegans	2096:2117	Caenorhabditis elegans	2096:2117	In this study, we describe a requirement of host monosaccharides, likely derived from host mucins, for the ability of Pseudomonas aeruginosa to colonize the intestine and ultimately cause death in Caenorhabditis elegans We also demonstrate that monosaccharides alter the ability of bacteria to bind to both Caenorhabditis elegans intestinal cells and human lung alveolar epithelial cells, suggesting that there are conserved mechanisms underlying host-pathogen interactions in a range of organisms.
32127446	10	61	theme	intestinal	2229:2238	arg1	cells					2240:2244	Caenorhabditis elegans intestinal cells	2206:2244	Caenorhabditis elegans intestinal cells	2206:2244	In this study, we describe a requirement of host monosaccharides, likely derived from host mucins, for the ability of Pseudomonas aeruginosa to colonize the intestine and ultimately cause death in Caenorhabditis elegans We also demonstrate that monosaccharides alter the ability of bacteria to bind to both Caenorhabditis elegans intestinal cells and human lung alveolar epithelial cells, suggesting that there are conserved mechanisms underlying host-pathogen interactions in a range of organisms.
32127446	6	62	theme	bacterial	1286:1294	arg1	pathogenesis					1296:1307	bacterial pathogenesis	1286:1307	bacterial pathogenesis	1286:1307	The monosaccharides identified in this study are found in a wide range of organisms where they act as host factors required for bacterial pathogenesis.
32127446	2	63	theme	structural	276:285	arg1	components					287:296	The major structural components	266:296	The major structural components of mucus	266:305	The major structural components of mucus are heavily glycosylated proteins called mucins.
32127446	2	63	theme	structural	276:285	arg1	proteins					332:339	heavily glycosylated proteins	311:339	heavily glycosylated proteins called mucins	311:353	The major structural components of mucus are heavily glycosylated proteins called mucins.
32127446	1	64	theme	epithelial	180:189	arg1	cells					191:195	lining epithelial cells	173:195	lining epithelial cells	173:195	One of the primary functions of the mucosal barrier, found lining epithelial cells, is to serve as a first-line of defense against microbial pathogens.
32127446	10	65	theme	epithelial	2270:2279	arg1	cells					2281:2285	human lung alveolar epithelial cells	2250:2285	human lung alveolar epithelial cells	2250:2285	In this study, we describe a requirement of host monosaccharides, likely derived from host mucins, for the ability of Pseudomonas aeruginosa to colonize the intestine and ultimately cause death in Caenorhabditis elegans We also demonstrate that monosaccharides alter the ability of bacteria to bind to both Caenorhabditis elegans intestinal cells and human lung alveolar epithelial cells, suggesting that there are conserved mechanisms underlying host-pathogen interactions in a range of organisms.
32127446	7	66	theme	acid	1349:1352	arg1	caps					1354:1357	sialic acid caps	1342:1357	sialic acid caps	1342:1357	While mucins in C. elegans lack sialic acid caps, which makes their monosaccharides readily available, they are capped in other species.
32127446	9	67	theme	immune	1883:1888	arg1	they					1788:1791	they	1788:1791	they	1788:1791	Mucins serve various functions, but importantly they aid in the clearance of pathogens and debris from epithelial barriers and serve as innate immune factors.
32127446	9	67	theme	immune	1883:1888	arg1	factors					1890:1896	innate immune factors	1876:1896	innate immune factors	1876:1896	Mucins serve various functions, but importantly they aid in the clearance of pathogens and debris from epithelial barriers and serve as innate immune factors.
32127446	5	68	theme	alveolar	1039:1046	arg1	cells					1059:1063	human lung alveolar epithelial cells	1028:1063	human lung alveolar epithelial cells	1028:1063	We found that the mucin MUL-1 and mucin-derived monosaccharides N-acetyl-galactosamine and N-acetylglucosamine are required for P. aeruginosa killing of Caenorhabditis elegans We also found that the defective adhesion of P. aeruginosa to human lung alveolar epithelial cells, deficient in the mucin MUC1, can be reversed by the addition of individual monosaccharides.
32127446	7	69	dep	lack	1337:1340	arg1	makes					1366:1370	makes	1366:1370	makes their monosaccharides readily available	1366:1410	While mucins in C. elegans lack sialic acid caps, which makes their monosaccharides readily available, they are capped in other species.
32127446	10	70	theme	lung	2256:2259	arg1	cells					2281:2285	human lung alveolar epithelial cells	2250:2285	human lung alveolar epithelial cells	2250:2285	In this study, we describe a requirement of host monosaccharides, likely derived from host mucins, for the ability of Pseudomonas aeruginosa to colonize the intestine and ultimately cause death in Caenorhabditis elegans We also demonstrate that monosaccharides alter the ability of bacteria to bind to both Caenorhabditis elegans intestinal cells and human lung alveolar epithelial cells, suggesting that there are conserved mechanisms underlying host-pathogen interactions in a range of organisms.
32127446	11	71	theme	better	2479:2484	arg1	approaches					2486:2495	better approaches	2479:2495	better approaches	2479:2495	By gaining a better understanding of pathogen-mucin interactions, we can develop better approaches to protect against pathogen infection.
32127446	4	72	theme	pathogen	608:615	arg1	virulence					585:593	the virulence	581:593	the virulence of the human pathogen Pseudomonas aeruginosa Here, we show data indicating that mucins not only play a role in host defense but that they can also be subverted by P. aeruginosa to cause disease	581:787	It has also been recently reported that individual mucins and derived glycans can attenuate the virulence of the human pathogen Pseudomonas aeruginosa Here, we show data indicating that mucins not only play a role in host defense but that they can also be subverted by P. aeruginosa to cause disease.
32127446	6	73	theme	host	1260:1263	arg1	they					1248:1251	they	1248:1251	they	1248:1251	The monosaccharides identified in this study are found in a wide range of organisms where they act as host factors required for bacterial pathogenesis.
32127446	6	73	theme	host	1260:1263	arg1	factors					1265:1271	host factors	1260:1271	host factors required for bacterial pathogenesis	1260:1307	The monosaccharides identified in this study are found in a wide range of organisms where they act as host factors required for bacterial pathogenesis.
32127446	8	74	theme	viscous	1692:1698	arg1	mucus					1667:1671	mucus	1667:1671	mucus	1667:1671	Pathogens such as P. aeruginosa that lack sialidases may rely on enzymes from other bacteria to utilize mucin-derived monosaccharides.IMPORTANCE One of the first lines of defense present at mucosal epithelial tissues is mucus, which is a highly viscous material formed by mucin glycoproteins.
32127446	8	74	theme	viscous	1692:1698	arg1	material					1700:1707	a highly viscous material	1683:1707	a highly viscous material formed by mucin glycoproteins	1683:1737	Pathogens such as P. aeruginosa that lack sialidases may rely on enzymes from other bacteria to utilize mucin-derived monosaccharides.IMPORTANCE One of the first lines of defense present at mucosal epithelial tissues is mucus, which is a highly viscous material formed by mucin glycoproteins.
32127446	10	75	dep	Caenorhabditis	2206:2219	arg1	elegans					2221:2227	elegans	2221:2227	elegans	2221:2227	In this study, we describe a requirement of host monosaccharides, likely derived from host mucins, for the ability of Pseudomonas aeruginosa to colonize the intestine and ultimately cause death in Caenorhabditis elegans We also demonstrate that monosaccharides alter the ability of bacteria to bind to both Caenorhabditis elegans intestinal cells and human lung alveolar epithelial cells, suggesting that there are conserved mechanisms underlying host-pathogen interactions in a range of organisms.
32127446	8	76	attach	present	1626:1632	arg1	tissues					1656:1662	mucosal epithelial tissues	1637:1662	mucosal epithelial tissues	1637:1662	Pathogens such as P. aeruginosa that lack sialidases may rely on enzymes from other bacteria to utilize mucin-derived monosaccharides.IMPORTANCE One of the first lines of defense present at mucosal epithelial tissues is mucus, which is a highly viscous material formed by mucin glycoproteins.
32127446	8	76	attach	present	1626:1632	arg2	defense					1618:1624	defense	1618:1624	defense present at mucosal epithelial tissues	1618:1662	Pathogens such as P. aeruginosa that lack sialidases may rely on enzymes from other bacteria to utilize mucin-derived monosaccharides.IMPORTANCE One of the first lines of defense present at mucosal epithelial tissues is mucus, which is a highly viscous material formed by mucin glycoproteins.
32127446	5	77	theme	aeruginosa	1014:1023	arg1	adhesion					999:1006	the defective adhesion	985:1006	the defective adhesion of P. aeruginosa to human lung alveolar epithelial cells, deficient in the mucin MUC1,	985:1093	We found that the mucin MUL-1 and mucin-derived monosaccharides N-acetyl-galactosamine and N-acetylglucosamine are required for P. aeruginosa killing of Caenorhabditis elegans We also found that the defective adhesion of P. aeruginosa to human lung alveolar epithelial cells, deficient in the mucin MUC1, can be reversed by the addition of individual monosaccharides.
32127446	5	78	theme	mucin	808:812	arg1	MUL-1					814:818	the mucin MUL-1 and mucin-derived monosaccharides	804:852	MUL-1	814:818	We found that the mucin MUL-1 and mucin-derived monosaccharides N-acetyl-galactosamine and N-acetylglucosamine are required for P. aeruginosa killing of Caenorhabditis elegans We also found that the defective adhesion of P. aeruginosa to human lung alveolar epithelial cells, deficient in the mucin MUC1, can be reversed by the addition of individual monosaccharides.
32127446	10	79	theme	host	1943:1946	arg1	monosaccharides					1948:1962	host monosaccharides	1943:1962	host monosaccharides	1943:1962	In this study, we describe a requirement of host monosaccharides, likely derived from host mucins, for the ability of Pseudomonas aeruginosa to colonize the intestine and ultimately cause death in Caenorhabditis elegans We also demonstrate that monosaccharides alter the ability of bacteria to bind to both Caenorhabditis elegans intestinal cells and human lung alveolar epithelial cells, suggesting that there are conserved mechanisms underlying host-pathogen interactions in a range of organisms.
32127446	5	80	theme	human	1028:1032	arg1	cells					1059:1063	human lung alveolar epithelial cells	1028:1063	human lung alveolar epithelial cells	1028:1063	We found that the mucin MUL-1 and mucin-derived monosaccharides N-acetyl-galactosamine and N-acetylglucosamine are required for P. aeruginosa killing of Caenorhabditis elegans We also found that the defective adhesion of P. aeruginosa to human lung alveolar epithelial cells, deficient in the mucin MUC1, can be reversed by the addition of individual monosaccharides.
32127446	8	81	theme	mucin	1719:1723	arg1	glycoproteins					1725:1737	mucin glycoproteins	1719:1737	mucin glycoproteins	1719:1737	Pathogens such as P. aeruginosa that lack sialidases may rely on enzymes from other bacteria to utilize mucin-derived monosaccharides.IMPORTANCE One of the first lines of defense present at mucosal epithelial tissues is mucus, which is a highly viscous material formed by mucin glycoproteins.
32127446	1	82	theme	functions	133:141	arg1	One					114:116	One	114:116	One	114:116	One of the primary functions of the mucosal barrier, found lining epithelial cells, is to serve as a first-line of defense against microbial pathogens.
32127446	1	82	theme	functions	133:141	arg1	first-line					215:224	a first-line	213:224	a first-line of defense against microbial pathogens	213:263	One of the primary functions of the mucosal barrier, found lining epithelial cells, is to serve as a first-line of defense against microbial pathogens.
32127446	1	82	theme	functions	133:141	arg1	functions					133:141	the primary functions	121:141	the primary functions of the mucosal barrier	121:164	One of the primary functions of the mucosal barrier, found lining epithelial cells, is to serve as a first-line of defense against microbial pathogens.
32127446	11	83	theme	pathogen-mucin	2435:2448	arg1	interactions					2450:2461	pathogen-mucin interactions	2435:2461	pathogen-mucin interactions	2435:2461	By gaining a better understanding of pathogen-mucin interactions, we can develop better approaches to protect against pathogen infection.
32127446	9	84	from	barriers	1854:1861	arg1	clearance					1804:1812	the clearance	1800:1812	the clearance of pathogens and debris from epithelial barriers	1800:1861	Mucins serve various functions, but importantly they aid in the clearance of pathogens and debris from epithelial barriers and serve as innate immune factors.
32127446	5	85	theme	mucin-derived	824:836	arg1	monosaccharides					838:852	the mucin MUL-1 and mucin-derived monosaccharides	804:852	monosaccharides	838:852	We found that the mucin MUL-1 and mucin-derived monosaccharides N-acetyl-galactosamine and N-acetylglucosamine are required for P. aeruginosa killing of Caenorhabditis elegans We also found that the defective adhesion of P. aeruginosa to human lung alveolar epithelial cells, deficient in the mucin MUC1, can be reversed by the addition of individual monosaccharides.
32127446	0	86	theme	Successful	92:101	arg1	Infection					103:111	a Successful Infection	90:111	a Successful Infection	90:111	Host Mucin Is Exploited by Pseudomonas aeruginosa To Provide Monosaccharides Required for a Successful Infection.
32127446	8	87	gly	glycoproteins	1725:1737	arg1	glycoproteins					1725:1737	mucin glycoproteins	1719:1737	mucin glycoproteins	1719:1737	Pathogens such as P. aeruginosa that lack sialidases may rely on enzymes from other bacteria to utilize mucin-derived monosaccharides.IMPORTANCE One of the first lines of defense present at mucosal epithelial tissues is mucus, which is a highly viscous material formed by mucin glycoproteins.
32127446	5	88	theme	deficient	1066:1074	arg1	cells					1059:1063	human lung alveolar epithelial cells	1028:1063	human lung alveolar epithelial cells	1028:1063	We found that the mucin MUL-1 and mucin-derived monosaccharides N-acetyl-galactosamine and N-acetylglucosamine are required for P. aeruginosa killing of Caenorhabditis elegans We also found that the defective adhesion of P. aeruginosa to human lung alveolar epithelial cells, deficient in the mucin MUC1, can be reversed by the addition of individual monosaccharides.
32127446	5	89	theme	Caenorhabditis	943:956	arg1	elegans					958:964	Caenorhabditis elegans	943:964	Caenorhabditis elegans	943:964	We found that the mucin MUL-1 and mucin-derived monosaccharides N-acetyl-galactosamine and N-acetylglucosamine are required for P. aeruginosa killing of Caenorhabditis elegans We also found that the defective adhesion of P. aeruginosa to human lung alveolar epithelial cells, deficient in the mucin MUC1, can be reversed by the addition of individual monosaccharides.
32127446	10	90	theme	host-pathogen	2346:2358	arg1	interactions					2360:2371	host-pathogen interactions	2346:2371	host-pathogen interactions	2346:2371	In this study, we describe a requirement of host monosaccharides, likely derived from host mucins, for the ability of Pseudomonas aeruginosa to colonize the intestine and ultimately cause death in Caenorhabditis elegans We also demonstrate that monosaccharides alter the ability of bacteria to bind to both Caenorhabditis elegans intestinal cells and human lung alveolar epithelial cells, suggesting that there are conserved mechanisms underlying host-pathogen interactions in a range of organisms.
32127446	7	91	from	mucins	1316:1321	arg1	elegans					1329:1335	C. elegans	1326:1335	C. elegans	1326:1335	While mucins in C. elegans lack sialic acid caps, which makes their monosaccharides readily available, they are capped in other species.
32127446	9	92	theme	various	1753:1759	arg1	functions					1761:1769	various functions	1753:1769	various functions	1753:1769	Mucins serve various functions, but importantly they aid in the clearance of pathogens and debris from epithelial barriers and serve as innate immune factors.
32127446	10	93	theme	bacteria	2181:2188	arg1	ability					2170:2176	the ability	2166:2176	the ability of bacteria to bind to both Caenorhabditis elegans intestinal cells and human lung alveolar epithelial cells	2166:2285	In this study, we describe a requirement of host monosaccharides, likely derived from host mucins, for the ability of Pseudomonas aeruginosa to colonize the intestine and ultimately cause death in Caenorhabditis elegans We also demonstrate that monosaccharides alter the ability of bacteria to bind to both Caenorhabditis elegans intestinal cells and human lung alveolar epithelial cells, suggesting that there are conserved mechanisms underlying host-pathogen interactions in a range of organisms.
32127446	5	94	link	mucin-derived	824:836	arg1	monosaccharides					838:852	the mucin MUL-1 and mucin-derived monosaccharides	804:852	monosaccharides	838:852	We found that the mucin MUL-1 and mucin-derived monosaccharides N-acetyl-galactosamine and N-acetylglucosamine are required for P. aeruginosa killing of Caenorhabditis elegans We also found that the defective adhesion of P. aeruginosa to human lung alveolar epithelial cells, deficient in the mucin MUC1, can be reversed by the addition of individual monosaccharides.
32127446	5	95	theme	P.	918:919	arg1	killing					932:938	P. aeruginosa killing	918:938	P. aeruginosa killing of Caenorhabditis elegans	918:964	We found that the mucin MUL-1 and mucin-derived monosaccharides N-acetyl-galactosamine and N-acetylglucosamine are required for P. aeruginosa killing of Caenorhabditis elegans We also found that the defective adhesion of P. aeruginosa to human lung alveolar epithelial cells, deficient in the mucin MUC1, can be reversed by the addition of individual monosaccharides.
32127446	10	96	dep	death	2087:2091	arg1	demonstrate					2127:2137	demonstrate	2127:2137	demonstrate that monosaccharides alter the ability of bacteria to bind to both Caenorhabditis elegans intestinal cells and human lung alveolar epithelial cells, suggesting that there are conserved mechanisms underlying host-pathogen interactions in a range of organisms	2127:2395	In this study, we describe a requirement of host monosaccharides, likely derived from host mucins, for the ability of Pseudomonas aeruginosa to colonize the intestine and ultimately cause death in Caenorhabditis elegans We also demonstrate that monosaccharides alter the ability of bacteria to bind to both Caenorhabditis elegans intestinal cells and human lung alveolar epithelial cells, suggesting that there are conserved mechanisms underlying host-pathogen interactions in a range of organisms.
32127446	5	97	theme	monosaccharides	1141:1155	arg1	addition					1118:1125	the addition	1114:1125	the addition of individual monosaccharides	1114:1155	We found that the mucin MUL-1 and mucin-derived monosaccharides N-acetyl-galactosamine and N-acetylglucosamine are required for P. aeruginosa killing of Caenorhabditis elegans We also found that the defective adhesion of P. aeruginosa to human lung alveolar epithelial cells, deficient in the mucin MUC1, can be reversed by the addition of individual monosaccharides.
32127446	3	98	theme	immune	396:401	arg1	system					403:408	the innate immune system	385:408	the innate immune system	385:408	Mucins are key components of the innate immune system as they aid in the clearance of pathogens and can decrease pathogen virulence.
32127446	7	99	dep	available	1402:1410	arg1	monosaccharides					1378:1392	their monosaccharides	1372:1392	their monosaccharides	1372:1392	While mucins in C. elegans lack sialic acid caps, which makes their monosaccharides readily available, they are capped in other species.
32127446	8	100	theme	other	1525:1529	arg1	bacteria					1531:1538	other bacteria	1525:1538	other bacteria	1525:1538	Pathogens such as P. aeruginosa that lack sialidases may rely on enzymes from other bacteria to utilize mucin-derived monosaccharides.IMPORTANCE One of the first lines of defense present at mucosal epithelial tissues is mucus, which is a highly viscous material formed by mucin glycoproteins.
32127446	2	101	gly	glycosylated	319:330	arg1	components					287:296	The major structural components	266:296	The major structural components of mucus	266:305	The major structural components of mucus are heavily glycosylated proteins called mucins.
32127446	2	101	gly	glycosylated	319:330	arg1	proteins					332:339	heavily glycosylated proteins	311:339	heavily glycosylated proteins called mucins	311:353	The major structural components of mucus are heavily glycosylated proteins called mucins.
32127446	8	102	from	tissues	1656:1662	arg1	present					1626:1632	present	1626:1632	present	1626:1632	Pathogens such as P. aeruginosa that lack sialidases may rely on enzymes from other bacteria to utilize mucin-derived monosaccharides.IMPORTANCE One of the first lines of defense present at mucosal epithelial tissues is mucus, which is a highly viscous material formed by mucin glycoproteins.
32127446	8	103	theme	lines	1609:1613	arg1	One					1592:1594	One	1592:1594	One	1592:1594	Pathogens such as P. aeruginosa that lack sialidases may rely on enzymes from other bacteria to utilize mucin-derived monosaccharides.IMPORTANCE One of the first lines of defense present at mucosal epithelial tissues is mucus, which is a highly viscous material formed by mucin glycoproteins.
32127446	8	103	theme	lines	1609:1613	arg1	lines					1609:1613	the first lines	1599:1613	the first lines of defense present at mucosal epithelial tissues	1599:1662	Pathogens such as P. aeruginosa that lack sialidases may rely on enzymes from other bacteria to utilize mucin-derived monosaccharides.IMPORTANCE One of the first lines of defense present at mucosal epithelial tissues is mucus, which is a highly viscous material formed by mucin glycoproteins.
32127446	2	104	theme	mucus	301:305	arg1	components					287:296	The major structural components	266:296	The major structural components of mucus	266:305	The major structural components of mucus are heavily glycosylated proteins called mucins.
32127446	2	104	theme	mucus	301:305	arg1	proteins					332:339	heavily glycosylated proteins	311:339	heavily glycosylated proteins called mucins	311:353	The major structural components of mucus are heavily glycosylated proteins called mucins.
32127446	8	105	theme	defense	1618:1624	arg1	lines					1609:1613	the first lines	1599:1613	the first lines of defense present at mucosal epithelial tissues	1599:1662	Pathogens such as P. aeruginosa that lack sialidases may rely on enzymes from other bacteria to utilize mucin-derived monosaccharides.IMPORTANCE One of the first lines of defense present at mucosal epithelial tissues is mucus, which is a highly viscous material formed by mucin glycoproteins.
32127446	10	106	theme	host	1985:1988	arg1	mucins					1990:1995	host mucins	1985:1995	host mucins	1985:1995	In this study, we describe a requirement of host monosaccharides, likely derived from host mucins, for the ability of Pseudomonas aeruginosa to colonize the intestine and ultimately cause death in Caenorhabditis elegans We also demonstrate that monosaccharides alter the ability of bacteria to bind to both Caenorhabditis elegans intestinal cells and human lung alveolar epithelial cells, suggesting that there are conserved mechanisms underlying host-pathogen interactions in a range of organisms.
32127446	8	107	theme	mucin-derived	1551:1563	arg1	monosaccharides.IMPORTANCE					1565:1590	mucin-derived monosaccharides.IMPORTANCE	1551:1590	mucin-derived monosaccharides.IMPORTANCE	1551:1590	Pathogens such as P. aeruginosa that lack sialidases may rely on enzymes from other bacteria to utilize mucin-derived monosaccharides.IMPORTANCE One of the first lines of defense present at mucosal epithelial tissues is mucus, which is a highly viscous material formed by mucin glycoproteins.
33161356	15	0	theme	calidiresistens	2833:2847	arg1	descriptions					2797:2808	the descriptions	2793:2808	the descriptions of S. alkaliphilus, S. calidiresistens and S. durbertensis	2793:2867	Data acquired in the present study have also been used to emend the descriptions of S. alkaliphilus, S. calidiresistens and S. durbertensis.
33161356	13	1	theme	well-supported	2337:2350	arg1	clade					2352:2356	a well-supported clade	2335:2356	a well-supported clade	2335:2356	Isolate IF17 and S. alkaliphilus DSM 42118T formed a well-supported clade in the phylogenomic tree, had almost identical digital G + C similarity values, produced long straight chains of smooth-surfaced spores and shared ANI and dDDH values (98.0 and 79.6%, respectively) consistent with their assignment to the same genomic species.
33161356	4	2	theme	spores	631:636	arg1	chains					621:626	straight chains	612:626	straight chains of spores with smooth surfaces	612:657	The isolates formed extensively branched substrate mycelia and aerial hyphae that differentiated in straight chains of spores with smooth surfaces.
33161356	10	3	theme	phenotypic	1855:1864	arg1	data					1866:1869	the genomic, phylogenetic and associated phenotypic data	1814:1869	the genomic, phylogenetic and associated phenotypic data	1814:1869	Consequently, on the basis of the genomic, phylogenetic and associated phenotypic data it is proposed that isolates OF1T, OF3 and OF8 be assigned to the genus Streptomyces as Streptomyces alkaliterrae sp.
33161356	7	4	theme	high	1134:1137	arg1	similarities					1148:1159	relatively high sequence similarities	1123:1159	relatively high sequence similarities	1123:1159	Isolates OF1T, OF3 and OF8 formed a distinct clade within the Streptomyces 16S rRNA gene tree sharing relatively high sequence similarities with the type strains of Streptomyces durbertensis (99.3%), Streptomyces palmae (98.1%) and Streptomyces xinghaiensis (98.3%), but can be distinguished from them using combinations of phenotypic properties.
33161356	13	5	theme	same	2596:2599	arg1	species					2609:2615	the same genomic species	2592:2615	the same genomic species	2592:2615	Isolate IF17 and S. alkaliphilus DSM 42118T formed a well-supported clade in the phylogenomic tree, had almost identical digital G + C similarity values, produced long straight chains of smooth-surfaced spores and shared ANI and dDDH values (98.0 and 79.6%, respectively) consistent with their assignment to the same genomic species.
33161356	13	6	theme	similarity	2419:2428	arg1	values					2430:2435	almost identical digital G + C similarity values	2388:2435	almost identical digital G + C similarity values	2388:2435	Isolate IF17 and S. alkaliphilus DSM 42118T formed a well-supported clade in the phylogenomic tree, had almost identical digital G + C similarity values, produced long straight chains of smooth-surfaced spores and shared ANI and dDDH values (98.0 and 79.6%, respectively) consistent with their assignment to the same genomic species.
33161356	7	7	theme	rRNA	1100:1103	arg1	tree					1110:1113	the Streptomyces 16S rRNA gene tree	1079:1113	the Streptomyces 16S rRNA gene tree	1079:1113	Isolates OF1T, OF3 and OF8 formed a distinct clade within the Streptomyces 16S rRNA gene tree sharing relatively high sequence similarities with the type strains of Streptomyces durbertensis (99.3%), Streptomyces palmae (98.1%) and Streptomyces xinghaiensis (98.3%), but can be distinguished from them using combinations of phenotypic properties.
33161356	9	8	theme	isolate	1638:1644	arg1	OF1T					1646:1649	isolate OF1T	1638:1649	isolate OF1T	1638:1649	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values calculated from the whole genome sequences of isolate OF1T and S. durbertensis DSM 104538T were low at 92.0% and 45.2%, respectively, indicating that they belong to different genomic species.
33161356	14	9	theme	authentic	2693:2701	arg1	IF19					2670:2673	IF19	2670:2673	IF19	2670:2673	In light of all of the data isolates IF11, IF17 and IF19 should be seen as authentic stains of S. alkalihilus.
33161356	14	9	theme	authentic	2693:2701	arg1	stains					2703:2708	authentic stains	2693:2708	authentic stains of S. alkalihilus	2693:2726	In light of all of the data isolates IF11, IF17 and IF19 should be seen as authentic stains of S. alkalihilus.
33161356	14	9	theme	authentic	2693:2701	arg1	IF17					2661:2664	IF17	2661:2664	IF17	2661:2664	In light of all of the data isolates IF11, IF17 and IF19 should be seen as authentic stains of S. alkalihilus.
33161356	4	10	theme	smooth	643:648	arg1	surfaces					650:657	smooth surfaces	643:657	smooth surfaces	643:657	The isolates formed extensively branched substrate mycelia and aerial hyphae that differentiated in straight chains of spores with smooth surfaces.
33161356	2	11	theme	alkaline	307:314	arg1	soil					316:319	an alkaline soil	304:319	an alkaline soil adjacent to a meteoric alkaline soda lake in India	304:370	A polyphasic study was undertaken to establish the taxonomic position of six representative streptomycetes isolated from an alkaline soil adjacent to a meteoric alkaline soda lake in India.
33161356	13	12	theme	S.	2301:2302	arg1	42118T					2321:2326	S. alkaliphilus DSM 42118T	2301:2326	S. alkaliphilus DSM 42118T	2301:2326	Isolate IF17 and S. alkaliphilus DSM 42118T formed a well-supported clade in the phylogenomic tree, had almost identical digital G + C similarity values, produced long straight chains of smooth-surfaced spores and shared ANI and dDDH values (98.0 and 79.6%, respectively) consistent with their assignment to the same genomic species.
33161356	2	13	from	lake	358:361	arg1	India					366:370	India	366:370	India	366:370	A polyphasic study was undertaken to establish the taxonomic position of six representative streptomycetes isolated from an alkaline soil adjacent to a meteoric alkaline soda lake in India.
33161356	5	14	contain	contained	665:673	arg1	They					660:663	They	660:663	They	660:663	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	5	14	contain	contained	665:673	arg2	acid					693:696	LL-diaminopimelic acid	675:696	LL-diaminopimelic acid in the wall peptidoglycan	675:722	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	7	15	theme	gene	1105:1108	arg1	tree					1110:1113	the Streptomyces 16S rRNA gene tree	1079:1113	the Streptomyces 16S rRNA gene tree	1079:1113	Isolates OF1T, OF3 and OF8 formed a distinct clade within the Streptomyces 16S rRNA gene tree sharing relatively high sequence similarities with the type strains of Streptomyces durbertensis (99.3%), Streptomyces palmae (98.1%) and Streptomyces xinghaiensis (98.3%), but can be distinguished from them using combinations of phenotypic properties.
33161356	12	16	from	clade	2238:2242	arg1	tree					2278:2281	the Streptomyces 16S rRNA gene tree	2247:2281	the Streptomyces 16S rRNA gene tree	2247:2281	Isolates IF11, IF17 and IF19, and S. alkaliphilus DSM 42118T were shown to belong to the same taxospecies and together with S. calidiresistens DSM 42108T comprised a well supported clade in the Streptomyces 16S rRNA gene tree.
33161356	11	17	theme	type	2044:2047	arg1	strain					2049:2054	the type strain	2040:2054	the type strain	2040:2054	nov. with strain OF1T (NCIMB 15195T =PCM 3001T) as the type strain.
33161356	11	18	theme	=PCM	2025:2028	arg1	3001T					2030:2034	NCIMB 15195T =PCM 3001T	2012:2034	NCIMB 15195T =PCM 3001T	2012:2034	nov. with strain OF1T (NCIMB 15195T =PCM 3001T) as the type strain.
33161356	11	18	theme	=PCM	2025:2028	arg1	OF1T					2006:2009	strain OF1T	1999:2009	strain OF1T (NCIMB 15195T =PCM 3001T)	1999:2035	nov. with strain OF1T (NCIMB 15195T =PCM 3001T) as the type strain.
33161356	7	19	theme	16S	1096:1098	arg1	tree					1110:1113	the Streptomyces 16S rRNA gene tree	1079:1113	the Streptomyces 16S rRNA gene tree	1079:1113	Isolates OF1T, OF3 and OF8 formed a distinct clade within the Streptomyces 16S rRNA gene tree sharing relatively high sequence similarities with the type strains of Streptomyces durbertensis (99.3%), Streptomyces palmae (98.1%) and Streptomyces xinghaiensis (98.3%), but can be distinguished from them using combinations of phenotypic properties.
33161356	6	20	dep	%	996:996	arg1	w/v					999:1001	w/v	999:1001	w/v	999:1001	The isolates grew well at 30 °C, pH 9 and in the presence of 3 to 5% (w/v) sodium chloride.
33161356	9	21	theme	104538T	1675:1681	arg1	sequences					1625:1633	the whole genome sequences	1608:1633	the whole genome sequences of isolate OF1T and S. durbertensis DSM 104538T	1608:1681	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values calculated from the whole genome sequences of isolate OF1T and S. durbertensis DSM 104538T were low at 92.0% and 45.2%, respectively, indicating that they belong to different genomic species.
33161356	14	22	theme	data	2641:2644	arg1	isolates					2646:2653	the data isolates IF11	2637:2658	the data isolates IF11	2637:2658	In light of all of the data isolates IF11, IF17 and IF19 should be seen as authentic stains of S. alkalihilus.
33161356	9	23	theme	DNA-DNA	1556:1562	arg1	dDDH					1579:1582	dDDH	1579:1582	dDDH	1579:1582	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values calculated from the whole genome sequences of isolate OF1T and S. durbertensis DSM 104538T were low at 92.0% and 45.2%, respectively, indicating that they belong to different genomic species.
33161356	9	23	theme	DNA-DNA	1556:1562	arg1	hybridization					1564:1576	digital DNA-DNA hybridization	1548:1576	digital DNA-DNA hybridization (dDDH)	1548:1583	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values calculated from the whole genome sequences of isolate OF1T and S. durbertensis DSM 104538T were low at 92.0% and 45.2%, respectively, indicating that they belong to different genomic species.
33161356	2	24	theme	adjacent	321:328	arg1	soil					316:319	an alkaline soil	304:319	an alkaline soil adjacent to a meteoric alkaline soda lake in India	304:370	A polyphasic study was undertaken to establish the taxonomic position of six representative streptomycetes isolated from an alkaline soil adjacent to a meteoric alkaline soda lake in India.
33161356	6	25	theme	5	995:995	arg1	%					996:996	%	996:996	%	996:996	The isolates grew well at 30 °C, pH 9 and in the presence of 3 to 5% (w/v) sodium chloride.
33161356	16	26	theme	gene	2970:2973	arg1	clusters					2975:2982	biosynthetic gene clusters	2957:2982	biosynthetic gene clusters	2957:2982	The genomes of isolates IF17, and OF1T, OF3 and OF8 contain relatively high numbers of biosynthetic gene clusters some of which were discontinously distributed indicating ones predicted to express for novel specialised metabolites.
33161356	7	27	theme	properties	1356:1365	arg1	combinations					1329:1340	combinations	1329:1340	combinations of phenotypic properties	1329:1365	Isolates OF1T, OF3 and OF8 formed a distinct clade within the Streptomyces 16S rRNA gene tree sharing relatively high sequence similarities with the type strains of Streptomyces durbertensis (99.3%), Streptomyces palmae (98.1%) and Streptomyces xinghaiensis (98.3%), but can be distinguished from them using combinations of phenotypic properties.
33161356	15	28	theme	alkaliphilus	2816:2827	arg1	descriptions					2797:2808	the descriptions	2793:2808	the descriptions of S. alkaliphilus, S. calidiresistens and S. durbertensis	2793:2867	Data acquired in the present study have also been used to emend the descriptions of S. alkaliphilus, S. calidiresistens and S. durbertensis.
33161356	5	29	theme	isoprene	791:798	arg1	units					800:804	nine isoprene units	786:804	nine isoprene units	786:804	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	9	30	theme	genomic	1767:1773	arg1	species					1775:1781	different genomic species	1757:1781	different genomic species	1757:1781	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values calculated from the whole genome sequences of isolate OF1T and S. durbertensis DSM 104538T were low at 92.0% and 45.2%, respectively, indicating that they belong to different genomic species.
33161356	6	31	theme	3	990:990	arg1	presence					978:985	the presence	974:985	the presence of 3 to 5% (w/v) sodium chloride	974:1018	The isolates grew well at 30 °C, pH 9 and in the presence of 3 to 5% (w/v) sodium chloride.
33161356	11	32	theme	15195T	2018:2023	arg1	3001T					2030:2034	NCIMB 15195T =PCM 3001T	2012:2034	NCIMB 15195T =PCM 3001T	2012:2034	nov. with strain OF1T (NCIMB 15195T =PCM 3001T) as the type strain.
33161356	11	32	theme	15195T	2018:2023	arg1	OF1T					2006:2009	strain OF1T	1999:2009	strain OF1T (NCIMB 15195T =PCM 3001T)	1999:2035	nov. with strain OF1T (NCIMB 15195T =PCM 3001T) as the type strain.
33161356	8	33	theme	isolates	1427:1434	arg1	sequences					1410:1418	draft genome sequences	1397:1418	draft genome sequences of the isolates and S. durbertensis DSM 104538T	1397:1466	A phylogenomic tree based on draft genome sequences of the isolates and S. durbertensis DSM 104538T confirmed the phylogenetic relationships.
33161356	12	34	theme	DSM	2200:2202	arg1	42108T					2204:2209	S. calidiresistens DSM 42108T	2181:2209	S. calidiresistens DSM 42108T	2181:2209	Isolates IF11, IF17 and IF19, and S. alkaliphilus DSM 42118T were shown to belong to the same taxospecies and together with S. calidiresistens DSM 42108T comprised a well supported clade in the Streptomyces 16S rRNA gene tree.
33161356	1	35	theme	alkaliphilus	109:120	arg1	descriptions					80:91	emended descriptions	72:91	emended descriptions of Streptomyces alkaliphilus, Streptomyces calidiresistens and Streptomyces durbertensis	72:180	nov., isolated from an alkaline soil, and emended descriptions of Streptomyces alkaliphilus, Streptomyces calidiresistens and Streptomyces durbertensis.
33161356	1	35	theme	alkaliphilus	109:120	arg1	nov.					30:33	nov.	30:33	nov.	30:33	nov., isolated from an alkaline soil, and emended descriptions of Streptomyces alkaliphilus, Streptomyces calidiresistens and Streptomyces durbertensis.
33161356	8	36	theme	S.	1440:1441	arg1	104538T					1460:1466	S. durbertensis DSM 104538T	1440:1466	S. durbertensis DSM 104538T	1440:1466	A phylogenomic tree based on draft genome sequences of the isolates and S. durbertensis DSM 104538T confirmed the phylogenetic relationships.
33161356	13	37	theme	G + C	2413:2417	arg1	values					2430:2435	almost identical digital G + C similarity values	2388:2435	almost identical digital G + C similarity values	2388:2435	Isolate IF17 and S. alkaliphilus DSM 42118T formed a well-supported clade in the phylogenomic tree, had almost identical digital G + C similarity values, produced long straight chains of smooth-surfaced spores and shared ANI and dDDH values (98.0 and 79.6%, respectively) consistent with their assignment to the same genomic species.
33161356	5	38	theme	octa-hydrogenated	750:766	arg1	acids					859:863	saturated, iso- and anteiso- fatty acids	824:863	saturated, iso- and anteiso- fatty acids	824:863	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	5	38	theme	octa-hydrogenated	750:766	arg1	lipid					922:926	the characteristic polar lipid	897:926	the characteristic polar lipid	897:926	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	5	38	theme	octa-hydrogenated	750:766	arg1	hexa-					741:745	hexa-	741:745	hexa-	741:745	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	5	38	theme	octa-hydrogenated	750:766	arg1	phosphatidylethanolamine					869:892	phosphatidylethanolamine	869:892	phosphatidylethanolamine	869:892	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	5	38	theme	octa-hydrogenated	750:766	arg1	amounts					813:819	major amounts	807:819	major amounts of saturated, iso- and anteiso- fatty acids	807:863	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	5	38	theme	octa-hydrogenated	750:766	arg1	menaquinones					768:779	octa-hydrogenated menaquinones	750:779	octa-hydrogenated menaquinones with nine isoprene units	750:804	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	2	39	theme	streptomycetes	275:288	arg1	position					244:251	the taxonomic position	230:251	the taxonomic position of six representative streptomycetes isolated from an alkaline soil adjacent to a meteoric alkaline soda lake in India	230:370	A polyphasic study was undertaken to establish the taxonomic position of six representative streptomycetes isolated from an alkaline soil adjacent to a meteoric alkaline soda lake in India.
33161356	16	40	theme	novel	3071:3075	arg1	metabolites					3089:3099	novel specialised metabolites	3071:3099	novel specialised metabolites	3071:3099	The genomes of isolates IF17, and OF1T, OF3 and OF8 contain relatively high numbers of biosynthetic gene clusters some of which were discontinously distributed indicating ones predicted to express for novel specialised metabolites.
33161356	2	41	attach	isolated	290:297	arg1	soil					316:319	an alkaline soil	304:319	an alkaline soil adjacent to a meteoric alkaline soda lake in India	304:370	A polyphasic study was undertaken to establish the taxonomic position of six representative streptomycetes isolated from an alkaline soil adjacent to a meteoric alkaline soda lake in India.
33161356	2	41	attach	isolated	290:297	arg2	streptomycetes					275:288	six representative streptomycetes	256:288	six representative streptomycetes isolated from an alkaline soil adjacent to a meteoric alkaline soda lake in India	256:370	A polyphasic study was undertaken to establish the taxonomic position of six representative streptomycetes isolated from an alkaline soil adjacent to a meteoric alkaline soda lake in India.
33161356	8	42	theme	DSM	1456:1458	arg1	104538T					1460:1466	S. durbertensis DSM 104538T	1440:1466	S. durbertensis DSM 104538T	1440:1466	A phylogenomic tree based on draft genome sequences of the isolates and S. durbertensis DSM 104538T confirmed the phylogenetic relationships.
33161356	14	43	theme	all	2630:2632	arg1	light					2621:2625	light	2621:2625	light of all of the data isolates IF11	2621:2658	In light of all of the data isolates IF11, IF17 and IF19 should be seen as authentic stains of S. alkalihilus.
33161356	13	44	dep	S.	2301:2302	arg1	alkaliphilus					2304:2315	alkaliphilus	2304:2315	alkaliphilus	2304:2315	Isolate IF17 and S. alkaliphilus DSM 42118T formed a well-supported clade in the phylogenomic tree, had almost identical digital G + C similarity values, produced long straight chains of smooth-surfaced spores and shared ANI and dDDH values (98.0 and 79.6%, respectively) consistent with their assignment to the same genomic species.
33161356	13	45	theme	98.0	2526:2529	arg1	%					2539:2539	98.0 and 79.6%	2526:2539	%	2539:2539	Isolate IF17 and S. alkaliphilus DSM 42118T formed a well-supported clade in the phylogenomic tree, had almost identical digital G + C similarity values, produced long straight chains of smooth-surfaced spores and shared ANI and dDDH values (98.0 and 79.6%, respectively) consistent with their assignment to the same genomic species.
33161356	10	46	theme	genus	1937:1941	arg1	Streptomyces					1943:1954	the genus Streptomyces	1933:1954	the genus Streptomyces	1933:1954	Consequently, on the basis of the genomic, phylogenetic and associated phenotypic data it is proposed that isolates OF1T, OF3 and OF8 be assigned to the genus Streptomyces as Streptomyces alkaliterrae sp.
33161356	7	47	theme	palmae	1234:1239	arg1	strains					1175:1181	the type strains	1166:1181	the type strains of Streptomyces durbertensis (99.3%), Streptomyces palmae (98.1%) and Streptomyces xinghaiensis (98.3%)	1166:1285	Isolates OF1T, OF3 and OF8 formed a distinct clade within the Streptomyces 16S rRNA gene tree sharing relatively high sequence similarities with the type strains of Streptomyces durbertensis (99.3%), Streptomyces palmae (98.1%) and Streptomyces xinghaiensis (98.3%), but can be distinguished from them using combinations of phenotypic properties.
33161356	9	48	theme	Average	1510:1516	arg1	ANI					1539:1541	ANI	1539:1541	ANI	1539:1541	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values calculated from the whole genome sequences of isolate OF1T and S. durbertensis DSM 104538T were low at 92.0% and 45.2%, respectively, indicating that they belong to different genomic species.
33161356	9	48	theme	Average	1510:1516	arg1	identity					1529:1536	Average nucleotide identity	1510:1536	Average nucleotide identity (ANI)	1510:1542	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values calculated from the whole genome sequences of isolate OF1T and S. durbertensis DSM 104538T were low at 92.0% and 45.2%, respectively, indicating that they belong to different genomic species.
33161356	9	49	from	%	1699:1699	arg1	low					1688:1690	low	1688:1690	low	1688:1690	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values calculated from the whole genome sequences of isolate OF1T and S. durbertensis DSM 104538T were low at 92.0% and 45.2%, respectively, indicating that they belong to different genomic species.
33161356	16	50	contain	contain	2922:2928	arg1	genomes					2874:2880	The genomes	2870:2880	The genomes of isolates IF17, and OF1T, OF3 and OF8	2870:2920	The genomes of isolates IF17, and OF1T, OF3 and OF8 contain relatively high numbers of biosynthetic gene clusters some of which were discontinously distributed indicating ones predicted to express for novel specialised metabolites.
33161356	16	50	contain	contain	2922:2928	arg2	numbers					2946:2952	relatively high numbers	2930:2952	relatively high numbers of biosynthetic gene clusters some of which were discontinously distributed indicating ones predicted to express for novel specialised metabolites	2930:3099	The genomes of isolates IF17, and OF1T, OF3 and OF8 contain relatively high numbers of biosynthetic gene clusters some of which were discontinously distributed indicating ones predicted to express for novel specialised metabolites.
33161356	5	51	theme	iso-	835:838	arg1	acids					859:863	saturated, iso- and anteiso- fatty acids	824:863	saturated, iso- and anteiso- fatty acids	824:863	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	1	52	theme	durbertensis	169:180	arg1	descriptions					80:91	emended descriptions	72:91	emended descriptions of Streptomyces alkaliphilus, Streptomyces calidiresistens and Streptomyces durbertensis	72:180	nov., isolated from an alkaline soil, and emended descriptions of Streptomyces alkaliphilus, Streptomyces calidiresistens and Streptomyces durbertensis.
33161356	1	52	theme	durbertensis	169:180	arg1	nov.					30:33	nov.	30:33	nov.	30:33	nov., isolated from an alkaline soil, and emended descriptions of Streptomyces alkaliphilus, Streptomyces calidiresistens and Streptomyces durbertensis.
33161356	3	53	theme	Chemotaxonomic	373:386	arg1	properties					416:425	Chemotaxonomic, cultural and morphological properties	373:425	Chemotaxonomic, cultural and morphological properties of the isolates	373:441	Chemotaxonomic, cultural and morphological properties of the isolates were consistent with their classification in the genus Streptomyces.
33161356	8	54	theme	phylogenetic	1482:1493	arg1	relationships					1495:1507	the phylogenetic relationships	1478:1507	the phylogenetic relationships	1478:1507	A phylogenomic tree based on draft genome sequences of the isolates and S. durbertensis DSM 104538T confirmed the phylogenetic relationships.
33161356	10	55	theme	alkaliterrae	1972:1983	arg1	sp					1985:1986	Streptomyces alkaliterrae sp	1959:1986	Streptomyces alkaliterrae sp	1959:1986	Consequently, on the basis of the genomic, phylogenetic and associated phenotypic data it is proposed that isolates OF1T, OF3 and OF8 be assigned to the genus Streptomyces as Streptomyces alkaliterrae sp.
33161356	10	55	theme	alkaliterrae	1972:1983	arg1	isolates					1891:1898	isolates OF1T, OF3 and OF8	1891:1916	isolates OF1T, OF3 and OF8	1891:1916	Consequently, on the basis of the genomic, phylogenetic and associated phenotypic data it is proposed that isolates OF1T, OF3 and OF8 be assigned to the genus Streptomyces as Streptomyces alkaliterrae sp.
33161356	1	56	theme	alkaline	53:60	arg1	soil					62:65	an alkaline soil	50:65	an alkaline soil	50:65	nov., isolated from an alkaline soil, and emended descriptions of Streptomyces alkaliphilus, Streptomyces calidiresistens and Streptomyces durbertensis.
33161356	13	57	contain	had	2384:2386	arg2	values					2430:2435	almost identical digital G + C similarity values	2388:2435	almost identical digital G + C similarity values	2388:2435	Isolate IF17 and S. alkaliphilus DSM 42118T formed a well-supported clade in the phylogenomic tree, had almost identical digital G + C similarity values, produced long straight chains of smooth-surfaced spores and shared ANI and dDDH values (98.0 and 79.6%, respectively) consistent with their assignment to the same genomic species.
33161356	13	57	contain	had	2384:2386	arg1	Isolate					2284:2290	Isolate IF17	2284:2295	Isolate IF17	2284:2295	Isolate IF17 and S. alkaliphilus DSM 42118T formed a well-supported clade in the phylogenomic tree, had almost identical digital G + C similarity values, produced long straight chains of smooth-surfaced spores and shared ANI and dDDH values (98.0 and 79.6%, respectively) consistent with their assignment to the same genomic species.
33161356	13	58	dep	values	2518:2523	arg1	%					2539:2539	98.0 and 79.6%	2526:2539	%	2539:2539	Isolate IF17 and S. alkaliphilus DSM 42118T formed a well-supported clade in the phylogenomic tree, had almost identical digital G + C similarity values, produced long straight chains of smooth-surfaced spores and shared ANI and dDDH values (98.0 and 79.6%, respectively) consistent with their assignment to the same genomic species.
33161356	3	59	theme	morphological	402:414	arg1	properties					416:425	Chemotaxonomic, cultural and morphological properties	373:425	Chemotaxonomic, cultural and morphological properties of the isolates	373:441	Chemotaxonomic, cultural and morphological properties of the isolates were consistent with their classification in the genus Streptomyces.
33161356	12	60	theme	DSM	2107:2109	arg1	42118T					2111:2116	S. alkaliphilus DSM 42118T	2091:2116	S. alkaliphilus DSM 42118T	2091:2116	Isolates IF11, IF17 and IF19, and S. alkaliphilus DSM 42118T were shown to belong to the same taxospecies and together with S. calidiresistens DSM 42108T comprised a well supported clade in the Streptomyces 16S rRNA gene tree.
33161356	12	60	theme	DSM	2107:2109	arg1	Isolates					2057:2064	Isolates IF11, IF17 and IF19, and S. alkaliphilus DSM 42118T	2057:2116	Isolates IF11, IF17 and IF19, and S. alkaliphilus DSM 42118T	2057:2116	Isolates IF11, IF17 and IF19, and S. alkaliphilus DSM 42118T were shown to belong to the same taxospecies and together with S. calidiresistens DSM 42108T comprised a well supported clade in the Streptomyces 16S rRNA gene tree.
33161356	0	61	theme	Streptomyces	0:11	arg1	sp					26:27	Streptomyces alkaliterrae sp	0:27	Streptomyces alkaliterrae sp.	0:28	Streptomyces alkaliterrae sp.
33161356	4	62	dep	formed	525:530	arg1	branched					544:551	branched	544:551	formed extensively branched substrate mycelia and aerial hyphae that differentiated in straight chains of spores with smooth surfaces	525:657	The isolates formed extensively branched substrate mycelia and aerial hyphae that differentiated in straight chains of spores with smooth surfaces.
33161356	14	63	theme	alkalihilus	2716:2726	arg1	IF19					2670:2673	IF19	2670:2673	IF19	2670:2673	In light of all of the data isolates IF11, IF17 and IF19 should be seen as authentic stains of S. alkalihilus.
33161356	14	63	theme	alkalihilus	2716:2726	arg1	stains					2703:2708	authentic stains	2693:2708	authentic stains of S. alkalihilus	2693:2726	In light of all of the data isolates IF11, IF17 and IF19 should be seen as authentic stains of S. alkalihilus.
33161356	14	63	theme	alkalihilus	2716:2726	arg1	IF17					2661:2664	IF17	2661:2664	IF17	2661:2664	In light of all of the data isolates IF11, IF17 and IF19 should be seen as authentic stains of S. alkalihilus.
33161356	1	64	theme	emended	72:78	arg1	descriptions					80:91	emended descriptions	72:91	emended descriptions of Streptomyces alkaliphilus, Streptomyces calidiresistens and Streptomyces durbertensis	72:180	nov., isolated from an alkaline soil, and emended descriptions of Streptomyces alkaliphilus, Streptomyces calidiresistens and Streptomyces durbertensis.
33161356	5	65	with	hexa-	741:745	arg1	units					800:804	nine isoprene units	786:804	nine isoprene units	786:804	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	3	66	theme	cultural	389:396	arg1	properties					416:425	Chemotaxonomic, cultural and morphological properties	373:425	Chemotaxonomic, cultural and morphological properties of the isolates	373:441	Chemotaxonomic, cultural and morphological properties of the isolates were consistent with their classification in the genus Streptomyces.
33161356	2	67	theme	representative	260:273	arg1	streptomycetes					275:288	six representative streptomycetes	256:288	six representative streptomycetes isolated from an alkaline soil adjacent to a meteoric alkaline soda lake in India	256:370	A polyphasic study was undertaken to establish the taxonomic position of six representative streptomycetes isolated from an alkaline soil adjacent to a meteoric alkaline soda lake in India.
33161356	12	68	theme	rRNA	2268:2271	arg1	tree					2278:2281	the Streptomyces 16S rRNA gene tree	2247:2281	the Streptomyces 16S rRNA gene tree	2247:2281	Isolates IF11, IF17 and IF19, and S. alkaliphilus DSM 42118T were shown to belong to the same taxospecies and together with S. calidiresistens DSM 42108T comprised a well supported clade in the Streptomyces 16S rRNA gene tree.
33161356	3	69	theme	genus	492:496	arg1	Streptomyces					498:509	the genus Streptomyces	488:509	the genus Streptomyces	488:509	Chemotaxonomic, cultural and morphological properties of the isolates were consistent with their classification in the genus Streptomyces.
33161356	3	70	theme	isolates	434:441	arg1	properties					416:425	Chemotaxonomic, cultural and morphological properties	373:425	Chemotaxonomic, cultural and morphological properties of the isolates	373:441	Chemotaxonomic, cultural and morphological properties of the isolates were consistent with their classification in the genus Streptomyces.
33161356	4	71	theme	straight	612:619	arg1	chains					621:626	straight chains	612:626	straight chains of spores with smooth surfaces	612:657	The isolates formed extensively branched substrate mycelia and aerial hyphae that differentiated in straight chains of spores with smooth surfaces.
33161356	2	72	theme	polyphasic	185:194	arg1	study					196:200	A polyphasic study	183:200	A polyphasic study	183:200	A polyphasic study was undertaken to establish the taxonomic position of six representative streptomycetes isolated from an alkaline soil adjacent to a meteoric alkaline soda lake in India.
33161356	8	73	theme	phylogenomic	1370:1381	arg1	tree					1383:1386	A phylogenomic tree	1368:1386	A phylogenomic tree based on draft genome sequences of the isolates and S. durbertensis DSM 104538T	1368:1466	A phylogenomic tree based on draft genome sequences of the isolates and S. durbertensis DSM 104538T confirmed the phylogenetic relationships.
33161356	16	74	dep	isolates	2885:2892	arg1	OF8					2918:2920	OF8	2918:2920	OF8	2918:2920	The genomes of isolates IF17, and OF1T, OF3 and OF8 contain relatively high numbers of biosynthetic gene clusters some of which were discontinously distributed indicating ones predicted to express for novel specialised metabolites.
33161356	16	74	dep	isolates	2885:2892	arg1	isolates					2885:2892	isolates IF17, and OF1T, OF3 and OF8	2885:2920	isolates IF17, and OF1T, OF3 and OF8	2885:2920	The genomes of isolates IF17, and OF1T, OF3 and OF8 contain relatively high numbers of biosynthetic gene clusters some of which were discontinously distributed indicating ones predicted to express for novel specialised metabolites.
33161356	16	74	dep	isolates	2885:2892	arg1	OF3					2910:2912	OF3	2910:2912	OF3	2910:2912	The genomes of isolates IF17, and OF1T, OF3 and OF8 contain relatively high numbers of biosynthetic gene clusters some of which were discontinously distributed indicating ones predicted to express for novel specialised metabolites.
33161356	16	74	dep	isolates	2885:2892	arg1	IF17					2894:2897	IF17	2894:2897	IF17	2894:2897	The genomes of isolates IF17, and OF1T, OF3 and OF8 contain relatively high numbers of biosynthetic gene clusters some of which were discontinously distributed indicating ones predicted to express for novel specialised metabolites.
33161356	16	74	dep	isolates	2885:2892	arg1	OF1T					2904:2907	OF1T	2904:2907	OF1T	2904:2907	The genomes of isolates IF17, and OF1T, OF3 and OF8 contain relatively high numbers of biosynthetic gene clusters some of which were discontinously distributed indicating ones predicted to express for novel specialised metabolites.
33161356	5	75	theme	anteiso-	844:851	arg1	acids					859:863	saturated, iso- and anteiso- fatty acids	824:863	saturated, iso- and anteiso- fatty acids	824:863	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	13	76	theme	straight	2452:2459	arg1	chains					2461:2466	long straight chains	2447:2466	long straight chains of smooth-surfaced spores	2447:2492	Isolate IF17 and S. alkaliphilus DSM 42118T formed a well-supported clade in the phylogenomic tree, had almost identical digital G + C similarity values, produced long straight chains of smooth-surfaced spores and shared ANI and dDDH values (98.0 and 79.6%, respectively) consistent with their assignment to the same genomic species.
33161356	5	77	theme	saturated	824:832	arg1	acids					859:863	saturated, iso- and anteiso- fatty acids	824:863	saturated, iso- and anteiso- fatty acids	824:863	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	12	78	theme	S.	2091:2092	arg1	42118T					2111:2116	S. alkaliphilus DSM 42118T	2091:2116	S. alkaliphilus DSM 42118T	2091:2116	Isolates IF11, IF17 and IF19, and S. alkaliphilus DSM 42118T were shown to belong to the same taxospecies and together with S. calidiresistens DSM 42108T comprised a well supported clade in the Streptomyces 16S rRNA gene tree.
33161356	12	78	theme	S.	2091:2092	arg1	Isolates					2057:2064	Isolates IF11, IF17 and IF19, and S. alkaliphilus DSM 42118T	2057:2116	Isolates IF11, IF17 and IF19, and S. alkaliphilus DSM 42118T	2057:2116	Isolates IF11, IF17 and IF19, and S. alkaliphilus DSM 42118T were shown to belong to the same taxospecies and together with S. calidiresistens DSM 42108T comprised a well supported clade in the Streptomyces 16S rRNA gene tree.
33161356	5	79	theme	acids	859:863	arg1	acids					859:863	saturated, iso- and anteiso- fatty acids	824:863	saturated, iso- and anteiso- fatty acids	824:863	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	5	79	theme	acids	859:863	arg1	lipid					922:926	the characteristic polar lipid	897:926	the characteristic polar lipid	897:926	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	5	79	theme	acids	859:863	arg1	hexa-					741:745	hexa-	741:745	hexa-	741:745	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	5	79	theme	acids	859:863	arg1	phosphatidylethanolamine					869:892	phosphatidylethanolamine	869:892	phosphatidylethanolamine	869:892	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	5	79	theme	acids	859:863	arg1	amounts					813:819	major amounts	807:819	major amounts of saturated, iso- and anteiso- fatty acids	807:863	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	5	79	theme	acids	859:863	arg1	menaquinones					768:779	octa-hydrogenated menaquinones	750:779	octa-hydrogenated menaquinones with nine isoprene units	750:804	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	13	80	theme	identical	2395:2403	arg1	values					2430:2435	almost identical digital G + C similarity values	2388:2435	almost identical digital G + C similarity values	2388:2435	Isolate IF17 and S. alkaliphilus DSM 42118T formed a well-supported clade in the phylogenomic tree, had almost identical digital G + C similarity values, produced long straight chains of smooth-surfaced spores and shared ANI and dDDH values (98.0 and 79.6%, respectively) consistent with their assignment to the same genomic species.
33161356	10	81	dep	data	1866:1869	arg1	the					1801:1803	the	1801:1803	the	1801:1803	Consequently, on the basis of the genomic, phylogenetic and associated phenotypic data it is proposed that isolates OF1T, OF3 and OF8 be assigned to the genus Streptomyces as Streptomyces alkaliterrae sp.
33161356	10	81	dep	data	1866:1869	arg1	basis					1805:1809	basis	1805:1809	basis	1805:1809	Consequently, on the basis of the genomic, phylogenetic and associated phenotypic data it is proposed that isolates OF1T, OF3 and OF8 be assigned to the genus Streptomyces as Streptomyces alkaliterrae sp.
33161356	10	82	theme	phylogenetic	1827:1838	arg1	data					1866:1869	the genomic, phylogenetic and associated phenotypic data	1814:1869	the genomic, phylogenetic and associated phenotypic data	1814:1869	Consequently, on the basis of the genomic, phylogenetic and associated phenotypic data it is proposed that isolates OF1T, OF3 and OF8 be assigned to the genus Streptomyces as Streptomyces alkaliterrae sp.
33161356	8	83	theme	draft	1397:1401	arg1	sequences					1410:1418	draft genome sequences	1397:1418	draft genome sequences of the isolates and S. durbertensis DSM 104538T	1397:1466	A phylogenomic tree based on draft genome sequences of the isolates and S. durbertensis DSM 104538T confirmed the phylogenetic relationships.
33161356	2	84	theme	soda	353:356	arg1	lake					358:361	a meteoric alkaline soda lake	333:361	a meteoric alkaline soda lake in India	333:370	A polyphasic study was undertaken to establish the taxonomic position of six representative streptomycetes isolated from an alkaline soil adjacent to a meteoric alkaline soda lake in India.
33161356	9	85	from	%	1709:1709	arg1	low					1688:1690	low	1688:1690	low	1688:1690	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values calculated from the whole genome sequences of isolate OF1T and S. durbertensis DSM 104538T were low at 92.0% and 45.2%, respectively, indicating that they belong to different genomic species.
33161356	12	86	theme	gene	2273:2276	arg1	tree					2278:2281	the Streptomyces 16S rRNA gene tree	2247:2281	the Streptomyces 16S rRNA gene tree	2247:2281	Isolates IF11, IF17 and IF19, and S. alkaliphilus DSM 42118T were shown to belong to the same taxospecies and together with S. calidiresistens DSM 42108T comprised a well supported clade in the Streptomyces 16S rRNA gene tree.
33161356	10	87	theme	genomic	1818:1824	arg1	data					1866:1869	the genomic, phylogenetic and associated phenotypic data	1814:1869	the genomic, phylogenetic and associated phenotypic data	1814:1869	Consequently, on the basis of the genomic, phylogenetic and associated phenotypic data it is proposed that isolates OF1T, OF3 and OF8 be assigned to the genus Streptomyces as Streptomyces alkaliterrae sp.
33161356	5	88	dep	characteristic	901:914	arg1	polar					916:920	polar	916:920	polar	916:920	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	5	89	theme	fatty	853:857	arg1	acids					859:863	saturated, iso- and anteiso- fatty acids	824:863	saturated, iso- and anteiso- fatty acids	824:863	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	9	90	theme	genome	1618:1623	arg1	sequences					1625:1633	the whole genome sequences	1608:1633	the whole genome sequences of isolate OF1T and S. durbertensis DSM 104538T	1608:1681	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values calculated from the whole genome sequences of isolate OF1T and S. durbertensis DSM 104538T were low at 92.0% and 45.2%, respectively, indicating that they belong to different genomic species.
33161356	15	91	theme	durbertensis	2856:2867	arg1	descriptions					2797:2808	the descriptions	2793:2808	the descriptions of S. alkaliphilus, S. calidiresistens and S. durbertensis	2793:2867	Data acquired in the present study have also been used to emend the descriptions of S. alkaliphilus, S. calidiresistens and S. durbertensis.
33161356	5	92	theme	wall	705:708	arg1	peptidoglycan					710:722	the wall peptidoglycan	701:722	the wall peptidoglycan	701:722	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	3	93	from	classification	470:483	arg1	Streptomyces					498:509	the genus Streptomyces	488:509	the genus Streptomyces	488:509	Chemotaxonomic, cultural and morphological properties of the isolates were consistent with their classification in the genus Streptomyces.
33161356	13	94	theme	digital	2405:2411	arg1	values					2430:2435	almost identical digital G + C similarity values	2388:2435	almost identical digital G + C similarity values	2388:2435	Isolate IF17 and S. alkaliphilus DSM 42118T formed a well-supported clade in the phylogenomic tree, had almost identical digital G + C similarity values, produced long straight chains of smooth-surfaced spores and shared ANI and dDDH values (98.0 and 79.6%, respectively) consistent with their assignment to the same genomic species.
33161356	10	95	theme	associated	1844:1853	arg1	data					1866:1869	the genomic, phylogenetic and associated phenotypic data	1814:1869	the genomic, phylogenetic and associated phenotypic data	1814:1869	Consequently, on the basis of the genomic, phylogenetic and associated phenotypic data it is proposed that isolates OF1T, OF3 and OF8 be assigned to the genus Streptomyces as Streptomyces alkaliterrae sp.
33161356	13	96	theme	genomic	2601:2607	arg1	species					2609:2615	the same genomic species	2592:2615	the same genomic species	2592:2615	Isolate IF17 and S. alkaliphilus DSM 42118T formed a well-supported clade in the phylogenomic tree, had almost identical digital G + C similarity values, produced long straight chains of smooth-surfaced spores and shared ANI and dDDH values (98.0 and 79.6%, respectively) consistent with their assignment to the same genomic species.
33161356	9	97	theme	OF1T	1646:1649	arg1	sequences					1625:1633	the whole genome sequences	1608:1633	the whole genome sequences of isolate OF1T and S. durbertensis DSM 104538T	1608:1681	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values calculated from the whole genome sequences of isolate OF1T and S. durbertensis DSM 104538T were low at 92.0% and 45.2%, respectively, indicating that they belong to different genomic species.
33161356	12	98	theme	16S	2264:2266	arg1	tree					2278:2281	the Streptomyces 16S rRNA gene tree	2247:2281	the Streptomyces 16S rRNA gene tree	2247:2281	Isolates IF11, IF17 and IF19, and S. alkaliphilus DSM 42118T were shown to belong to the same taxospecies and together with S. calidiresistens DSM 42108T comprised a well supported clade in the Streptomyces 16S rRNA gene tree.
33161356	5	99	theme	LL-diaminopimelic	675:691	arg1	acid					693:696	LL-diaminopimelic acid	675:696	LL-diaminopimelic acid in the wall peptidoglycan	675:722	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	16	100	theme	isolates	2885:2892	arg1	genomes					2874:2880	The genomes	2870:2880	The genomes of isolates IF17, and OF1T, OF3 and OF8	2870:2920	The genomes of isolates IF17, and OF1T, OF3 and OF8 contain relatively high numbers of biosynthetic gene clusters some of which were discontinously distributed indicating ones predicted to express for novel specialised metabolites.
33161356	9	101	dep	S.	1655:1656	arg1	durbertensis					1658:1669	durbertensis	1658:1669	durbertensis	1658:1669	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values calculated from the whole genome sequences of isolate OF1T and S. durbertensis DSM 104538T were low at 92.0% and 45.2%, respectively, indicating that they belong to different genomic species.
33161356	9	102	theme	S.	1655:1656	arg1	104538T					1675:1681	S. durbertensis DSM 104538T	1655:1681	S. durbertensis DSM 104538T	1655:1681	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values calculated from the whole genome sequences of isolate OF1T and S. durbertensis DSM 104538T were low at 92.0% and 45.2%, respectively, indicating that they belong to different genomic species.
33161356	13	103	theme	79.6	2535:2538	arg1	%					2539:2539	98.0 and 79.6%	2526:2539	%	2539:2539	Isolate IF17 and S. alkaliphilus DSM 42118T formed a well-supported clade in the phylogenomic tree, had almost identical digital G + C similarity values, produced long straight chains of smooth-surfaced spores and shared ANI and dDDH values (98.0 and 79.6%, respectively) consistent with their assignment to the same genomic species.
33161356	7	104	theme	Streptomyces	1083:1094	arg1	tree					1110:1113	the Streptomyces 16S rRNA gene tree	1079:1113	the Streptomyces 16S rRNA gene tree	1079:1113	Isolates OF1T, OF3 and OF8 formed a distinct clade within the Streptomyces 16S rRNA gene tree sharing relatively high sequence similarities with the type strains of Streptomyces durbertensis (99.3%), Streptomyces palmae (98.1%) and Streptomyces xinghaiensis (98.3%), but can be distinguished from them using combinations of phenotypic properties.
33161356	2	105	theme	meteoric	335:342	arg1	lake					358:361	a meteoric alkaline soda lake	333:361	a meteoric alkaline soda lake in India	333:370	A polyphasic study was undertaken to establish the taxonomic position of six representative streptomycetes isolated from an alkaline soil adjacent to a meteoric alkaline soda lake in India.
33161356	9	106	theme	DSM	1671:1673	arg1	104538T					1675:1681	S. durbertensis DSM 104538T	1655:1681	S. durbertensis DSM 104538T	1655:1681	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values calculated from the whole genome sequences of isolate OF1T and S. durbertensis DSM 104538T were low at 92.0% and 45.2%, respectively, indicating that they belong to different genomic species.
33161356	13	107	theme	phylogenomic	2365:2376	arg1	tree					2378:2381	the phylogenomic tree	2361:2381	the phylogenomic tree	2361:2381	Isolate IF17 and S. alkaliphilus DSM 42118T formed a well-supported clade in the phylogenomic tree, had almost identical digital G + C similarity values, produced long straight chains of smooth-surfaced spores and shared ANI and dDDH values (98.0 and 79.6%, respectively) consistent with their assignment to the same genomic species.
33161356	5	108	theme	characteristic	901:914	arg1	acids					859:863	saturated, iso- and anteiso- fatty acids	824:863	saturated, iso- and anteiso- fatty acids	824:863	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	5	108	theme	characteristic	901:914	arg1	lipid					922:926	the characteristic polar lipid	897:926	the characteristic polar lipid	897:926	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	5	108	theme	characteristic	901:914	arg1	hexa-					741:745	hexa-	741:745	hexa-	741:745	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	5	108	theme	characteristic	901:914	arg1	phosphatidylethanolamine					869:892	phosphatidylethanolamine	869:892	phosphatidylethanolamine	869:892	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	5	108	theme	characteristic	901:914	arg1	amounts					813:819	major amounts	807:819	major amounts of saturated, iso- and anteiso- fatty acids	807:863	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	5	108	theme	characteristic	901:914	arg1	menaquinones					768:779	octa-hydrogenated menaquinones	750:779	octa-hydrogenated menaquinones with nine isoprene units	750:804	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	6	109	theme	%	996:996	arg1	chloride					1011:1018	5% (w/v) sodium chloride	995:1018	5% (w/v) sodium chloride	995:1018	The isolates grew well at 30 °C, pH 9 and in the presence of 3 to 5% (w/v) sodium chloride.
33161356	12	110	theme	supported	2228:2236	arg1	clade					2238:2242	a well supported clade	2221:2242	a well supported clade in the Streptomyces 16S rRNA gene tree	2221:2281	Isolates IF11, IF17 and IF19, and S. alkaliphilus DSM 42118T were shown to belong to the same taxospecies and together with S. calidiresistens DSM 42108T comprised a well supported clade in the Streptomyces 16S rRNA gene tree.
33161356	5	111	with	phosphatidylethanolamine	869:892	arg1	units					800:804	nine isoprene units	786:804	nine isoprene units	786:804	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	9	112	theme	identity	1529:1536	arg1	values					1585:1590	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values	1510:1590	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values calculated from the whole genome sequences of isolate OF1T and S. durbertensis DSM 104538T	1510:1681	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values calculated from the whole genome sequences of isolate OF1T and S. durbertensis DSM 104538T were low at 92.0% and 45.2%, respectively, indicating that they belong to different genomic species.
33161356	7	113	theme	distinct	1057:1064	arg1	clade					1066:1070	a distinct clade	1055:1070	a distinct clade within the Streptomyces 16S rRNA gene tree sharing relatively high sequence similarities with the type strains of Streptomyces durbertensis (99.3%), Streptomyces palmae (98.1%) and Streptomyces xinghaiensis (98.3%)	1055:1285	Isolates OF1T, OF3 and OF8 formed a distinct clade within the Streptomyces 16S rRNA gene tree sharing relatively high sequence similarities with the type strains of Streptomyces durbertensis (99.3%), Streptomyces palmae (98.1%) and Streptomyces xinghaiensis (98.3%), but can be distinguished from them using combinations of phenotypic properties.
33161356	13	114	theme	spores	2487:2492	arg1	chains					2461:2466	long straight chains	2447:2466	long straight chains of smooth-surfaced spores	2447:2492	Isolate IF17 and S. alkaliphilus DSM 42118T formed a well-supported clade in the phylogenomic tree, had almost identical digital G + C similarity values, produced long straight chains of smooth-surfaced spores and shared ANI and dDDH values (98.0 and 79.6%, respectively) consistent with their assignment to the same genomic species.
33161356	12	115	theme	S.	2181:2182	arg1	42108T					2204:2209	S. calidiresistens DSM 42108T	2181:2209	S. calidiresistens DSM 42108T	2181:2209	Isolates IF11, IF17 and IF19, and S. alkaliphilus DSM 42118T were shown to belong to the same taxospecies and together with S. calidiresistens DSM 42108T comprised a well supported clade in the Streptomyces 16S rRNA gene tree.
33161356	5	116	with	amounts	813:819	arg1	units					800:804	nine isoprene units	786:804	nine isoprene units	786:804	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	3	117	with	consistent	448:457	arg1	classification					470:483	their classification	464:483	their classification in the genus Streptomyces	464:509	Chemotaxonomic, cultural and morphological properties of the isolates were consistent with their classification in the genus Streptomyces.
33161356	2	118	theme	taxonomic	234:242	arg1	position					244:251	the taxonomic position	230:251	the taxonomic position of six representative streptomycetes isolated from an alkaline soil adjacent to a meteoric alkaline soda lake in India	230:370	A polyphasic study was undertaken to establish the taxonomic position of six representative streptomycetes isolated from an alkaline soil adjacent to a meteoric alkaline soda lake in India.
33161356	4	119	theme	substrate	553:561	arg1	mycelia					563:569	substrate mycelia	553:569	substrate mycelia	553:569	The isolates formed extensively branched substrate mycelia and aerial hyphae that differentiated in straight chains of spores with smooth surfaces.
33161356	11	120	theme	strain	1999:2004	arg1	3001T					2030:2034	NCIMB 15195T =PCM 3001T	2012:2034	NCIMB 15195T =PCM 3001T	2012:2034	nov. with strain OF1T (NCIMB 15195T =PCM 3001T) as the type strain.
33161356	11	120	theme	strain	1999:2004	arg1	OF1T					2006:2009	strain OF1T	1999:2009	strain OF1T (NCIMB 15195T =PCM 3001T)	1999:2035	nov. with strain OF1T (NCIMB 15195T =PCM 3001T) as the type strain.
33161356	8	121	theme	durbertensis	1443:1454	arg1	104538T					1460:1466	S. durbertensis DSM 104538T	1440:1466	S. durbertensis DSM 104538T	1440:1466	A phylogenomic tree based on draft genome sequences of the isolates and S. durbertensis DSM 104538T confirmed the phylogenetic relationships.
33161356	7	122	theme	phenotypic	1345:1354	arg1	properties					1356:1365	phenotypic properties	1345:1365	phenotypic properties	1345:1365	Isolates OF1T, OF3 and OF8 formed a distinct clade within the Streptomyces 16S rRNA gene tree sharing relatively high sequence similarities with the type strains of Streptomyces durbertensis (99.3%), Streptomyces palmae (98.1%) and Streptomyces xinghaiensis (98.3%), but can be distinguished from them using combinations of phenotypic properties.
33161356	14	123	dep	isolates	2646:2653	arg1	IF11					2655:2658	IF11	2655:2658	IF11	2655:2658	In light of all of the data isolates IF11, IF17 and IF19 should be seen as authentic stains of S. alkalihilus.
33161356	11	124	theme	NCIMB	2012:2016	arg1	3001T					2030:2034	NCIMB 15195T =PCM 3001T	2012:2034	NCIMB 15195T =PCM 3001T	2012:2034	nov. with strain OF1T (NCIMB 15195T =PCM 3001T) as the type strain.
33161356	11	124	theme	NCIMB	2012:2016	arg1	OF1T					2006:2009	strain OF1T	1999:2009	strain OF1T (NCIMB 15195T =PCM 3001T)	1999:2035	nov. with strain OF1T (NCIMB 15195T =PCM 3001T) as the type strain.
33161356	8	125	theme	104538T	1460:1466	arg1	sequences					1410:1418	draft genome sequences	1397:1418	draft genome sequences of the isolates and S. durbertensis DSM 104538T	1397:1466	A phylogenomic tree based on draft genome sequences of the isolates and S. durbertensis DSM 104538T confirmed the phylogenetic relationships.
33161356	15	126	used	used	2779:2782	arg2	Data					2729:2732	Data	2729:2732	Data acquired in the present study	2729:2762	Data acquired in the present study have also been used to emend the descriptions of S. alkaliphilus, S. calidiresistens and S. durbertensis.
33161356	4	127	with	spores	631:636	arg1	surfaces					650:657	smooth surfaces	643:657	smooth surfaces	643:657	The isolates formed extensively branched substrate mycelia and aerial hyphae that differentiated in straight chains of spores with smooth surfaces.
33161356	12	128	dep	S.	2091:2092	arg1	alkaliphilus					2094:2105	alkaliphilus	2094:2105	alkaliphilus	2094:2105	Isolates IF11, IF17 and IF19, and S. alkaliphilus DSM 42118T were shown to belong to the same taxospecies and together with S. calidiresistens DSM 42108T comprised a well supported clade in the Streptomyces 16S rRNA gene tree.
33161356	1	129	theme	calidiresistens	136:150	arg1	descriptions					80:91	emended descriptions	72:91	emended descriptions of Streptomyces alkaliphilus, Streptomyces calidiresistens and Streptomyces durbertensis	72:180	nov., isolated from an alkaline soil, and emended descriptions of Streptomyces alkaliphilus, Streptomyces calidiresistens and Streptomyces durbertensis.
33161356	1	129	theme	calidiresistens	136:150	arg1	nov.					30:33	nov.	30:33	nov.	30:33	nov., isolated from an alkaline soil, and emended descriptions of Streptomyces alkaliphilus, Streptomyces calidiresistens and Streptomyces durbertensis.
33161356	13	130	theme	dDDH	2513:2516	arg1	values					2518:2523	ANI and dDDH values	2505:2523	values	2518:2523	Isolate IF17 and S. alkaliphilus DSM 42118T formed a well-supported clade in the phylogenomic tree, had almost identical digital G + C similarity values, produced long straight chains of smooth-surfaced spores and shared ANI and dDDH values (98.0 and 79.6%, respectively) consistent with their assignment to the same genomic species.
33161356	15	131	theme	present	2750:2756	arg1	study					2758:2762	the present study	2746:2762	the present study	2746:2762	Data acquired in the present study have also been used to emend the descriptions of S. alkaliphilus, S. calidiresistens and S. durbertensis.
33161356	16	132	theme	biosynthetic	2957:2968	arg1	clusters					2975:2982	biosynthetic gene clusters	2957:2982	biosynthetic gene clusters	2957:2982	The genomes of isolates IF17, and OF1T, OF3 and OF8 contain relatively high numbers of biosynthetic gene clusters some of which were discontinously distributed indicating ones predicted to express for novel specialised metabolites.
33161356	16	133	theme	specialised	3077:3087	arg1	metabolites					3089:3099	novel specialised metabolites	3071:3099	novel specialised metabolites	3071:3099	The genomes of isolates IF17, and OF1T, OF3 and OF8 contain relatively high numbers of biosynthetic gene clusters some of which were discontinously distributed indicating ones predicted to express for novel specialised metabolites.
33161356	9	134	theme	nucleotide	1518:1527	arg1	ANI					1539:1541	ANI	1539:1541	ANI	1539:1541	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values calculated from the whole genome sequences of isolate OF1T and S. durbertensis DSM 104538T were low at 92.0% and 45.2%, respectively, indicating that they belong to different genomic species.
33161356	9	134	theme	nucleotide	1518:1527	arg1	identity					1529:1536	Average nucleotide identity	1510:1536	Average nucleotide identity (ANI)	1510:1542	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values calculated from the whole genome sequences of isolate OF1T and S. durbertensis DSM 104538T were low at 92.0% and 45.2%, respectively, indicating that they belong to different genomic species.
33161356	12	135	theme	calidiresistens	2184:2198	arg1	42108T					2204:2209	S. calidiresistens DSM 42108T	2181:2209	S. calidiresistens DSM 42108T	2181:2209	Isolates IF11, IF17 and IF19, and S. alkaliphilus DSM 42118T were shown to belong to the same taxospecies and together with S. calidiresistens DSM 42108T comprised a well supported clade in the Streptomyces 16S rRNA gene tree.
33161356	12	136	theme	Streptomyces	2251:2262	arg1	tree					2278:2281	the Streptomyces 16S rRNA gene tree	2247:2281	the Streptomyces 16S rRNA gene tree	2247:2281	Isolates IF11, IF17 and IF19, and S. alkaliphilus DSM 42118T were shown to belong to the same taxospecies and together with S. calidiresistens DSM 42108T comprised a well supported clade in the Streptomyces 16S rRNA gene tree.
33161356	13	137	theme	ANI	2505:2507	arg1	values					2518:2523	ANI and dDDH values	2505:2523	values	2518:2523	Isolate IF17 and S. alkaliphilus DSM 42118T formed a well-supported clade in the phylogenomic tree, had almost identical digital G + C similarity values, produced long straight chains of smooth-surfaced spores and shared ANI and dDDH values (98.0 and 79.6%, respectively) consistent with their assignment to the same genomic species.
33161356	16	138	theme	clusters	2975:2982	arg1	numbers					2946:2952	relatively high numbers	2930:2952	relatively high numbers of biosynthetic gene clusters some of which were discontinously distributed indicating ones predicted to express for novel specialised metabolites	2930:3099	The genomes of isolates IF17, and OF1T, OF3 and OF8 contain relatively high numbers of biosynthetic gene clusters some of which were discontinously distributed indicating ones predicted to express for novel specialised metabolites.
33161356	13	139	theme	DSM	2317:2319	arg1	42118T					2321:2326	S. alkaliphilus DSM 42118T	2301:2326	S. alkaliphilus DSM 42118T	2301:2326	Isolate IF17 and S. alkaliphilus DSM 42118T formed a well-supported clade in the phylogenomic tree, had almost identical digital G + C similarity values, produced long straight chains of smooth-surfaced spores and shared ANI and dDDH values (98.0 and 79.6%, respectively) consistent with their assignment to the same genomic species.
33161356	6	140	theme	sodium	1004:1009	arg1	chloride					1011:1018	5% (w/v) sodium chloride	995:1018	5% (w/v) sodium chloride	995:1018	The isolates grew well at 30 °C, pH 9 and in the presence of 3 to 5% (w/v) sodium chloride.
33161356	12	141	theme	same	2146:2149	arg1	taxospecies					2151:2161	the same taxospecies	2142:2161	the same taxospecies	2142:2161	Isolates IF11, IF17 and IF19, and S. alkaliphilus DSM 42118T were shown to belong to the same taxospecies and together with S. calidiresistens DSM 42108T comprised a well supported clade in the Streptomyces 16S rRNA gene tree.
33161356	7	142	dep	Isolates	1021:1028	arg1	OF1T					1030:1033	OF1T	1030:1033	OF1T	1030:1033	Isolates OF1T, OF3 and OF8 formed a distinct clade within the Streptomyces 16S rRNA gene tree sharing relatively high sequence similarities with the type strains of Streptomyces durbertensis (99.3%), Streptomyces palmae (98.1%) and Streptomyces xinghaiensis (98.3%), but can be distinguished from them using combinations of phenotypic properties.
33161356	7	142	dep	Isolates	1021:1028	arg1	OF8					1044:1046	OF8	1044:1046	OF8	1044:1046	Isolates OF1T, OF3 and OF8 formed a distinct clade within the Streptomyces 16S rRNA gene tree sharing relatively high sequence similarities with the type strains of Streptomyces durbertensis (99.3%), Streptomyces palmae (98.1%) and Streptomyces xinghaiensis (98.3%), but can be distinguished from them using combinations of phenotypic properties.
33161356	7	142	dep	Isolates	1021:1028	arg1	Isolates					1021:1028	Isolates OF1T, OF3 and OF8	1021:1046	Isolates OF1T, OF3 and OF8	1021:1046	Isolates OF1T, OF3 and OF8 formed a distinct clade within the Streptomyces 16S rRNA gene tree sharing relatively high sequence similarities with the type strains of Streptomyces durbertensis (99.3%), Streptomyces palmae (98.1%) and Streptomyces xinghaiensis (98.3%), but can be distinguished from them using combinations of phenotypic properties.
33161356	7	142	dep	Isolates	1021:1028	arg1	OF3					1036:1038	OF3	1036:1038	OF3	1036:1038	Isolates OF1T, OF3 and OF8 formed a distinct clade within the Streptomyces 16S rRNA gene tree sharing relatively high sequence similarities with the type strains of Streptomyces durbertensis (99.3%), Streptomyces palmae (98.1%) and Streptomyces xinghaiensis (98.3%), but can be distinguished from them using combinations of phenotypic properties.
33161356	1	143	attach	isolated	36:43	arg1	soil					62:65	an alkaline soil	50:65	an alkaline soil	50:65	nov., isolated from an alkaline soil, and emended descriptions of Streptomyces alkaliphilus, Streptomyces calidiresistens and Streptomyces durbertensis.
33161356	1	143	attach	isolated	36:43	arg2	nov.					30:33	nov.	30:33	nov.	30:33	nov., isolated from an alkaline soil, and emended descriptions of Streptomyces alkaliphilus, Streptomyces calidiresistens and Streptomyces durbertensis.
33161356	5	144	theme	major	807:811	arg1	acids					859:863	saturated, iso- and anteiso- fatty acids	824:863	saturated, iso- and anteiso- fatty acids	824:863	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	5	144	theme	major	807:811	arg1	lipid					922:926	the characteristic polar lipid	897:926	the characteristic polar lipid	897:926	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	5	144	theme	major	807:811	arg1	hexa-					741:745	hexa-	741:745	hexa-	741:745	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	5	144	theme	major	807:811	arg1	phosphatidylethanolamine					869:892	phosphatidylethanolamine	869:892	phosphatidylethanolamine	869:892	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	5	144	theme	major	807:811	arg1	amounts					813:819	major amounts	807:819	major amounts of saturated, iso- and anteiso- fatty acids	807:863	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	5	144	theme	major	807:811	arg1	menaquinones					768:779	octa-hydrogenated menaquinones	750:779	octa-hydrogenated menaquinones with nine isoprene units	750:804	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	10	145	theme	Streptomyces	1959:1970	arg1	sp					1985:1986	Streptomyces alkaliterrae sp	1959:1986	Streptomyces alkaliterrae sp	1959:1986	Consequently, on the basis of the genomic, phylogenetic and associated phenotypic data it is proposed that isolates OF1T, OF3 and OF8 be assigned to the genus Streptomyces as Streptomyces alkaliterrae sp.
33161356	10	145	theme	Streptomyces	1959:1970	arg1	isolates					1891:1898	isolates OF1T, OF3 and OF8	1891:1916	isolates OF1T, OF3 and OF8	1891:1916	Consequently, on the basis of the genomic, phylogenetic and associated phenotypic data it is proposed that isolates OF1T, OF3 and OF8 be assigned to the genus Streptomyces as Streptomyces alkaliterrae sp.
33161356	7	146	theme	durbertensis	1199:1210	arg1	strains					1175:1181	the type strains	1166:1181	the type strains of Streptomyces durbertensis (99.3%), Streptomyces palmae (98.1%) and Streptomyces xinghaiensis (98.3%)	1166:1285	Isolates OF1T, OF3 and OF8 formed a distinct clade within the Streptomyces 16S rRNA gene tree sharing relatively high sequence similarities with the type strains of Streptomyces durbertensis (99.3%), Streptomyces palmae (98.1%) and Streptomyces xinghaiensis (98.3%), but can be distinguished from them using combinations of phenotypic properties.
33161356	0	147	theme	alkaliterrae	13:24	arg1	sp					26:27	Streptomyces alkaliterrae sp	0:27	Streptomyces alkaliterrae sp.	0:28	Streptomyces alkaliterrae sp.
33161356	9	148	theme	digital	1548:1554	arg1	dDDH					1579:1582	dDDH	1579:1582	dDDH	1579:1582	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values calculated from the whole genome sequences of isolate OF1T and S. durbertensis DSM 104538T were low at 92.0% and 45.2%, respectively, indicating that they belong to different genomic species.
33161356	9	148	theme	digital	1548:1554	arg1	hybridization					1564:1576	digital DNA-DNA hybridization	1548:1576	digital DNA-DNA hybridization (dDDH)	1548:1583	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values calculated from the whole genome sequences of isolate OF1T and S. durbertensis DSM 104538T were low at 92.0% and 45.2%, respectively, indicating that they belong to different genomic species.
33161356	9	149	theme	different	1757:1765	arg1	species					1775:1781	different genomic species	1757:1781	different genomic species	1757:1781	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values calculated from the whole genome sequences of isolate OF1T and S. durbertensis DSM 104538T were low at 92.0% and 45.2%, respectively, indicating that they belong to different genomic species.
33161356	7	150	theme	type	1170:1173	arg1	strains					1175:1181	the type strains	1166:1181	the type strains of Streptomyces durbertensis (99.3%), Streptomyces palmae (98.1%) and Streptomyces xinghaiensis (98.3%)	1166:1285	Isolates OF1T, OF3 and OF8 formed a distinct clade within the Streptomyces 16S rRNA gene tree sharing relatively high sequence similarities with the type strains of Streptomyces durbertensis (99.3%), Streptomyces palmae (98.1%) and Streptomyces xinghaiensis (98.3%), but can be distinguished from them using combinations of phenotypic properties.
33161356	9	151	theme	hybridization	1564:1576	arg1	values					1585:1590	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values	1510:1590	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values calculated from the whole genome sequences of isolate OF1T and S. durbertensis DSM 104538T	1510:1681	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values calculated from the whole genome sequences of isolate OF1T and S. durbertensis DSM 104538T were low at 92.0% and 45.2%, respectively, indicating that they belong to different genomic species.
33161356	11	152	with	nov.	1989:1992	arg1	3001T					2030:2034	NCIMB 15195T =PCM 3001T	2012:2034	NCIMB 15195T =PCM 3001T	2012:2034	nov. with strain OF1T (NCIMB 15195T =PCM 3001T) as the type strain.
33161356	11	152	with	nov.	1989:1992	arg1	OF1T					2006:2009	strain OF1T	1999:2009	strain OF1T (NCIMB 15195T =PCM 3001T)	1999:2035	nov. with strain OF1T (NCIMB 15195T =PCM 3001T) as the type strain.
33161356	10	153	dep	isolates	1891:1898	arg1	OF3					1906:1908	OF3	1906:1908	OF3	1906:1908	Consequently, on the basis of the genomic, phylogenetic and associated phenotypic data it is proposed that isolates OF1T, OF3 and OF8 be assigned to the genus Streptomyces as Streptomyces alkaliterrae sp.
33161356	10	153	dep	isolates	1891:1898	arg1	OF1T					1900:1903	OF1T	1900:1903	OF1T	1900:1903	Consequently, on the basis of the genomic, phylogenetic and associated phenotypic data it is proposed that isolates OF1T, OF3 and OF8 be assigned to the genus Streptomyces as Streptomyces alkaliterrae sp.
33161356	10	153	dep	isolates	1891:1898	arg1	OF8					1914:1916	OF8	1914:1916	OF8	1914:1916	Consequently, on the basis of the genomic, phylogenetic and associated phenotypic data it is proposed that isolates OF1T, OF3 and OF8 be assigned to the genus Streptomyces as Streptomyces alkaliterrae sp.
33161356	10	153	dep	isolates	1891:1898	arg1	isolates					1891:1898	isolates OF1T, OF3 and OF8	1891:1916	isolates OF1T, OF3 and OF8	1891:1916	Consequently, on the basis of the genomic, phylogenetic and associated phenotypic data it is proposed that isolates OF1T, OF3 and OF8 be assigned to the genus Streptomyces as Streptomyces alkaliterrae sp.
33161356	13	154	theme	long	2447:2450	arg1	chains					2461:2466	long straight chains	2447:2466	long straight chains of smooth-surfaced spores	2447:2492	Isolate IF17 and S. alkaliphilus DSM 42118T formed a well-supported clade in the phylogenomic tree, had almost identical digital G + C similarity values, produced long straight chains of smooth-surfaced spores and shared ANI and dDDH values (98.0 and 79.6%, respectively) consistent with their assignment to the same genomic species.
33161356	5	155	from	acid	693:696	arg1	peptidoglycan					710:722	the wall peptidoglycan	701:722	the wall peptidoglycan	701:722	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	5	156	with	menaquinones	768:779	arg1	units					800:804	nine isoprene units	786:804	nine isoprene units	786:804	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	4	157	theme	aerial	575:580	arg1	hyphae					582:587	aerial hyphae	575:587	aerial hyphae	575:587	The isolates formed extensively branched substrate mycelia and aerial hyphae that differentiated in straight chains of spores with smooth surfaces.
33161356	7	158	theme	sequence	1139:1146	arg1	similarities					1148:1159	relatively high sequence similarities	1123:1159	relatively high sequence similarities	1123:1159	Isolates OF1T, OF3 and OF8 formed a distinct clade within the Streptomyces 16S rRNA gene tree sharing relatively high sequence similarities with the type strains of Streptomyces durbertensis (99.3%), Streptomyces palmae (98.1%) and Streptomyces xinghaiensis (98.3%), but can be distinguished from them using combinations of phenotypic properties.
33161356	7	159	theme	xinghaiensis	1266:1277	arg1	strains					1175:1181	the type strains	1166:1181	the type strains of Streptomyces durbertensis (99.3%), Streptomyces palmae (98.1%) and Streptomyces xinghaiensis (98.3%)	1166:1285	Isolates OF1T, OF3 and OF8 formed a distinct clade within the Streptomyces 16S rRNA gene tree sharing relatively high sequence similarities with the type strains of Streptomyces durbertensis (99.3%), Streptomyces palmae (98.1%) and Streptomyces xinghaiensis (98.3%), but can be distinguished from them using combinations of phenotypic properties.
33161356	12	160	dep	Isolates	2057:2064	arg1	IF17					2072:2075	IF17	2072:2075	IF17	2072:2075	Isolates IF11, IF17 and IF19, and S. alkaliphilus DSM 42118T were shown to belong to the same taxospecies and together with S. calidiresistens DSM 42108T comprised a well supported clade in the Streptomyces 16S rRNA gene tree.
33161356	12	160	dep	Isolates	2057:2064	arg1	42118T					2111:2116	S. alkaliphilus DSM 42118T	2091:2116	S. alkaliphilus DSM 42118T	2091:2116	Isolates IF11, IF17 and IF19, and S. alkaliphilus DSM 42118T were shown to belong to the same taxospecies and together with S. calidiresistens DSM 42108T comprised a well supported clade in the Streptomyces 16S rRNA gene tree.
33161356	12	160	dep	Isolates	2057:2064	arg1	IF11					2066:2069	IF11	2066:2069	IF11	2066:2069	Isolates IF11, IF17 and IF19, and S. alkaliphilus DSM 42118T were shown to belong to the same taxospecies and together with S. calidiresistens DSM 42108T comprised a well supported clade in the Streptomyces 16S rRNA gene tree.
33161356	12	160	dep	Isolates	2057:2064	arg1	Isolates					2057:2064	Isolates IF11, IF17 and IF19, and S. alkaliphilus DSM 42118T	2057:2116	Isolates IF11, IF17 and IF19, and S. alkaliphilus DSM 42118T	2057:2116	Isolates IF11, IF17 and IF19, and S. alkaliphilus DSM 42118T were shown to belong to the same taxospecies and together with S. calidiresistens DSM 42108T comprised a well supported clade in the Streptomyces 16S rRNA gene tree.
33161356	5	161	dep	contained	665:673	arg1	produced					725:732	produced	725:732	produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid	725:926	They contained LL-diaminopimelic acid in the wall peptidoglycan, produced either hexa- or octa-hydrogenated menaquinones with nine isoprene units, major amounts of saturated, iso- and anteiso- fatty acids and phosphatidylethanolamine as the characteristic polar lipid.
33161356	13	162	with	consistent	2556:2565	arg1	assignment					2578:2587	their assignment	2572:2587	their assignment to the same genomic species	2572:2615	Isolate IF17 and S. alkaliphilus DSM 42118T formed a well-supported clade in the phylogenomic tree, had almost identical digital G + C similarity values, produced long straight chains of smooth-surfaced spores and shared ANI and dDDH values (98.0 and 79.6%, respectively) consistent with their assignment to the same genomic species.
33161356	2	163	theme	alkaline	344:351	arg1	lake					358:361	a meteoric alkaline soda lake	333:361	a meteoric alkaline soda lake in India	333:370	A polyphasic study was undertaken to establish the taxonomic position of six representative streptomycetes isolated from an alkaline soil adjacent to a meteoric alkaline soda lake in India.
33161356	16	164	theme	high	2941:2944	arg1	numbers					2946:2952	relatively high numbers	2930:2952	relatively high numbers of biosynthetic gene clusters some of which were discontinously distributed indicating ones predicted to express for novel specialised metabolites	2930:3099	The genomes of isolates IF17, and OF1T, OF3 and OF8 contain relatively high numbers of biosynthetic gene clusters some of which were discontinously distributed indicating ones predicted to express for novel specialised metabolites.
33161356	9	165	theme	whole	1612:1616	arg1	sequences					1625:1633	the whole genome sequences	1608:1633	the whole genome sequences of isolate OF1T and S. durbertensis DSM 104538T	1608:1681	Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values calculated from the whole genome sequences of isolate OF1T and S. durbertensis DSM 104538T were low at 92.0% and 45.2%, respectively, indicating that they belong to different genomic species.
33161356	8	166	theme	genome	1403:1408	arg1	sequences					1410:1418	draft genome sequences	1397:1418	draft genome sequences of the isolates and S. durbertensis DSM 104538T	1397:1466	A phylogenomic tree based on draft genome sequences of the isolates and S. durbertensis DSM 104538T confirmed the phylogenetic relationships.
33161356	13	167	theme	smooth-surfaced	2471:2485	arg1	spores					2487:2492	smooth-surfaced spores	2471:2492	smooth-surfaced spores	2471:2492	Isolate IF17 and S. alkaliphilus DSM 42118T formed a well-supported clade in the phylogenomic tree, had almost identical digital G + C similarity values, produced long straight chains of smooth-surfaced spores and shared ANI and dDDH values (98.0 and 79.6%, respectively) consistent with their assignment to the same genomic species.
33566377	0	0	theme	TNFα-dependent	83:96	arg1	inflammation					98:109	inflammation	98:109	inflammation	98:109	Deletion or inhibition of SphK1 mitigates fulminant hepatic failure by suppressing TNFα-dependent inflammation and apoptosis.
33566377	10	1	from	macrophages	1617:1627	arg1	secretion					1602:1610	TNFα secretion	1597:1610	TNFα secretion from macrophages	1597:1627	Our findings suggest that SphK1 regulates TNFα secretion from macrophages and inhibition or deletion of SphK1 mitigated ALF.
33566377	6	2	theme	decreased	1010:1018	arg1	expression					1020:1029	decreased expression	1010:1029	decreased expression of the pro-inflammatory cytokine TNFα	1010:1067	Moreover, repression of ALF in SphK1-/- mice correlated with decreased expression of the pro-inflammatory cytokine TNFα.
33566377	5	3	theme	wild-type	864:872	arg1	mice					887:890	wild-type and SphK2-/- mice	864:890	wild-type and SphK2-/- mice	864:890	Whereas GalN/LPS treatment-induced hepatic activation of NF-κB and JNK in wild-type and SphK2-/- mice, these signaling pathways were reduced in SphK1-/- mice.
33566377	1	4	theme	severe	159:164	arg1	dysfunction					172:182	severe liver dysfunction	159:182	severe liver dysfunction that can lead to multi-organ failure and death	159:229	Acute liver failure (ALF) causes severe liver dysfunction that can lead to multi-organ failure and death.
33566377	7	5	theme	liver-resident	1186:1199	arg1	cells					1201:1205	host liver-resident cells	1181:1205	host liver-resident cells	1181:1205	Adoptive transfer experiments indicated that SphK1 in bone marrow-derived infiltrating immune cells but not in host liver-resident cells, contribute to the development of ALF.
33566377	8	6	theme	anti-inflammatory	1421:1437	arg1	phenotype					1439:1447	the anti-inflammatory phenotype	1417:1447	the anti-inflammatory phenotype	1417:1447	Interestingly, LPS-induced TNFα production was drastically suppressed in SphK1-deleted macrophages, whereas IL-10 expression was markedly enhanced, suggesting a switch to the anti-inflammatory phenotype.
33566377	4	7	theme	aminotransferase	718:733	arg1	levels					735:740	serum alanine aminotransferase levels	704:740	serum alanine aminotransferase levels	704:740	Here we report that deletion of SphK1, but not SphK2, dramatically decreased GalN/LPS-induced liver damage, hepatic apoptosis, serum alanine aminotransferase levels, and mortality rate compared to wild-type mice.
33566377	3	8	theme	mouse	528:532	arg1	ALF					482:484	D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced ALF	426:484	D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced ALF	426:484	This study examines the role of SphK1 in D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced ALF, which is a well-established experimental mouse model that mimics the fulminant hepatitis.
33566377	3	8	theme	mouse	528:532	arg1	model					534:538	a well-established experimental mouse model	496:538	a well-established experimental mouse model that mimics the fulminant hepatitis	496:574	This study examines the role of SphK1 in D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced ALF, which is a well-established experimental mouse model that mimics the fulminant hepatitis.
33566377	5	9	theme	SphK1-/-	934:941	arg1	mice					943:946	SphK1-/- mice	934:946	SphK1-/- mice	934:946	Whereas GalN/LPS treatment-induced hepatic activation of NF-κB and JNK in wild-type and SphK2-/- mice, these signaling pathways were reduced in SphK1-/- mice.
33566377	4	10	theme	alanine	710:716	arg1	levels					735:740	serum alanine aminotransferase levels	704:740	serum alanine aminotransferase levels	704:740	Here we report that deletion of SphK1, but not SphK2, dramatically decreased GalN/LPS-induced liver damage, hepatic apoptosis, serum alanine aminotransferase levels, and mortality rate compared to wild-type mice.
33566377	10	11	theme	TNFα	1597:1600	arg1	secretion					1602:1610	TNFα secretion	1597:1610	TNFα secretion from macrophages	1597:1627	Our findings suggest that SphK1 regulates TNFα secretion from macrophages and inhibition or deletion of SphK1 mitigated ALF.
33566377	1	12	theme	Acute	126:130	arg1	failure					138:144	Acute liver failure	126:144	Acute liver failure (ALF)	126:150	Acute liver failure (ALF) causes severe liver dysfunction that can lead to multi-organ failure and death.
33566377	1	12	theme	Acute	126:130	arg1	ALF					147:149	ALF	147:149	ALF	147:149	Acute liver failure (ALF) causes severe liver dysfunction that can lead to multi-organ failure and death.
33566377	7	13	theme	Adoptive	1070:1077	arg1	experiments					1088:1098	Adoptive transfer experiments	1070:1098	Adoptive transfer experiments	1070:1098	Adoptive transfer experiments indicated that SphK1 in bone marrow-derived infiltrating immune cells but not in host liver-resident cells, contribute to the development of ALF.
33566377	6	14	theme	cytokine	1055:1062	arg1	TNFα					1064:1067	the pro-inflammatory cytokine TNFα	1034:1067	the pro-inflammatory cytokine TNFα	1034:1067	Moreover, repression of ALF in SphK1-/- mice correlated with decreased expression of the pro-inflammatory cytokine TNFα.
33566377	1	15	theme	liver	166:170	arg1	dysfunction					172:182	severe liver dysfunction	159:182	severe liver dysfunction that can lead to multi-organ failure and death	159:229	Acute liver failure (ALF) causes severe liver dysfunction that can lead to multi-organ failure and death.
33566377	7	16	theme	host	1181:1184	arg1	cells					1201:1205	host liver-resident cells	1181:1205	host liver-resident cells	1181:1205	Adoptive transfer experiments indicated that SphK1 in bone marrow-derived infiltrating immune cells but not in host liver-resident cells, contribute to the development of ALF.
33566377	8	17	theme	SphK1-deleted	1319:1331	arg1	macrophages					1333:1343	SphK1-deleted macrophages	1319:1343	SphK1-deleted macrophages	1319:1343	Interestingly, LPS-induced TNFα production was drastically suppressed in SphK1-deleted macrophages, whereas IL-10 expression was markedly enhanced, suggesting a switch to the anti-inflammatory phenotype.
33566377	7	18	theme	transfer	1079:1086	arg1	experiments					1088:1098	Adoptive transfer experiments	1070:1098	Adoptive transfer experiments	1070:1098	Adoptive transfer experiments indicated that SphK1 in bone marrow-derived infiltrating immune cells but not in host liver-resident cells, contribute to the development of ALF.
33566377	5	19	theme	signaling	899:907	arg1	pathways					909:916	these signaling pathways	893:916	these signaling pathways	893:916	Whereas GalN/LPS treatment-induced hepatic activation of NF-κB and JNK in wild-type and SphK2-/- mice, these signaling pathways were reduced in SphK1-/- mice.
33566377	6	20	theme	TNFα	1064:1067	arg1	expression					1020:1029	decreased expression	1010:1029	decreased expression of the pro-inflammatory cytokine TNFα	1010:1067	Moreover, repression of ALF in SphK1-/- mice correlated with decreased expression of the pro-inflammatory cytokine TNFα.
33566377	4	21	theme	SphK1	609:613	arg1	deletion					597:604	deletion	597:604	deletion of SphK1	597:613	Here we report that deletion of SphK1, but not SphK2, dramatically decreased GalN/LPS-induced liver damage, hepatic apoptosis, serum alanine aminotransferase levels, and mortality rate compared to wild-type mice.
33566377	11	22	theme	potent	1688:1693	arg1	agent					1749:1753	a therapeutic agent	1735:1753	a therapeutic agent for fulminant hepatitis	1735:1777	Thus, a potent inhibitor of SphK1 could potentially be a therapeutic agent for fulminant hepatitis.
33566377	11	22	theme	potent	1688:1693	arg1	inhibitor					1695:1703	a potent inhibitor	1686:1703	a potent inhibitor of SphK1	1686:1712	Thus, a potent inhibitor of SphK1 could potentially be a therapeutic agent for fulminant hepatitis.
33566377	2	23	theme	hepatocyte	308:317	arg1	injury					319:324	hepatocyte injury	308:324	hepatocyte injury	308:324	Previous studies suggest that sphingosine kinase 1 (SphK1) protects against hepatocyte injury, yet not much is still known about its involvement in ALF.
33566377	5	24	theme	NF-κB	847:851	arg1	activation					833:842	treatment-induced hepatic activation	807:842	treatment-induced hepatic activation of NF-κB and JNK in wild-type and SphK2-/- mice	807:890	Whereas GalN/LPS treatment-induced hepatic activation of NF-κB and JNK in wild-type and SphK2-/- mice, these signaling pathways were reduced in SphK1-/- mice.
33566377	6	25	from	repression	959:968	arg1	mice					989:992	SphK1-/- mice	980:992	SphK1-/- mice	980:992	Moreover, repression of ALF in SphK1-/- mice correlated with decreased expression of the pro-inflammatory cytokine TNFα.
33566377	4	26	theme	hepatic	685:691	arg1	apoptosis					693:701	hepatic apoptosis	685:701	hepatic apoptosis	685:701	Here we report that deletion of SphK1, but not SphK2, dramatically decreased GalN/LPS-induced liver damage, hepatic apoptosis, serum alanine aminotransferase levels, and mortality rate compared to wild-type mice.
33566377	5	27	theme	treatment-induced	807:823	arg1	activation					833:842	treatment-induced hepatic activation	807:842	treatment-induced hepatic activation of NF-κB and JNK in wild-type and SphK2-/- mice	807:890	Whereas GalN/LPS treatment-induced hepatic activation of NF-κB and JNK in wild-type and SphK2-/- mice, these signaling pathways were reduced in SphK1-/- mice.
33566377	7	28	theme	ALF	1241:1243	arg1	development					1226:1236	the development	1222:1236	the development of ALF	1222:1243	Adoptive transfer experiments indicated that SphK1 in bone marrow-derived infiltrating immune cells but not in host liver-resident cells, contribute to the development of ALF.
33566377	3	29	theme	well-established	498:513	arg1	ALF					482:484	D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced ALF	426:484	D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced ALF	426:484	This study examines the role of SphK1 in D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced ALF, which is a well-established experimental mouse model that mimics the fulminant hepatitis.
33566377	3	29	theme	well-established	498:513	arg1	model					534:538	a well-established experimental mouse model	496:538	a well-established experimental mouse model that mimics the fulminant hepatitis	496:574	This study examines the role of SphK1 in D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced ALF, which is a well-established experimental mouse model that mimics the fulminant hepatitis.
33566377	4	30	theme	wild-type	774:782	arg1	mice					784:787	wild-type mice	774:787	wild-type mice	774:787	Here we report that deletion of SphK1, but not SphK2, dramatically decreased GalN/LPS-induced liver damage, hepatic apoptosis, serum alanine aminotransferase levels, and mortality rate compared to wild-type mice.
33566377	9	31	theme	SphK1	1485:1489	arg1	inhibitor					1491:1499	a specific SphK1 inhibitor	1474:1499	a specific SphK1 inhibitor	1474:1499	Finally, treatment with a specific SphK1 inhibitor ameliorated inflammation and protected mice from ALF.
33566377	3	32	theme	experimental	515:526	arg1	ALF					482:484	D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced ALF	426:484	D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced ALF	426:484	This study examines the role of SphK1 in D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced ALF, which is a well-established experimental mouse model that mimics the fulminant hepatitis.
33566377	3	32	theme	experimental	515:526	arg1	model					534:538	a well-established experimental mouse model	496:538	a well-established experimental mouse model that mimics the fulminant hepatitis	496:574	This study examines the role of SphK1 in D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced ALF, which is a well-established experimental mouse model that mimics the fulminant hepatitis.
33566377	5	33	theme	JNK	857:859	arg1	activation					833:842	treatment-induced hepatic activation	807:842	treatment-induced hepatic activation of NF-κB and JNK in wild-type and SphK2-/- mice	807:890	Whereas GalN/LPS treatment-induced hepatic activation of NF-κB and JNK in wild-type and SphK2-/- mice, these signaling pathways were reduced in SphK1-/- mice.
33566377	7	34	theme	marrow-derived	1129:1142	arg1	cells					1164:1168	bone marrow-derived infiltrating immune cells	1124:1168	bone marrow-derived infiltrating immune cells	1124:1168	Adoptive transfer experiments indicated that SphK1 in bone marrow-derived infiltrating immune cells but not in host liver-resident cells, contribute to the development of ALF.
33566377	4	35	theme	GalN/LPS-induced	654:669	arg1	damage					677:682	GalN/LPS-induced liver damage	654:682	GalN/LPS-induced liver damage	654:682	Here we report that deletion of SphK1, but not SphK2, dramatically decreased GalN/LPS-induced liver damage, hepatic apoptosis, serum alanine aminotransferase levels, and mortality rate compared to wild-type mice.
33566377	1	36	theme	multi-organ	201:211	arg1	failure					213:219	failure	213:219	failure	213:219	Acute liver failure (ALF) causes severe liver dysfunction that can lead to multi-organ failure and death.
33566377	2	37	theme	Previous	232:239	arg1	studies					241:247	Previous studies	232:247	Previous studies	232:247	Previous studies suggest that sphingosine kinase 1 (SphK1) protects against hepatocyte injury, yet not much is still known about its involvement in ALF.
33566377	11	38	theme	therapeutic	1737:1747	arg1	inhibitor					1695:1703	a potent inhibitor	1686:1703	a potent inhibitor of SphK1	1686:1712	Thus, a potent inhibitor of SphK1 could potentially be a therapeutic agent for fulminant hepatitis.
33566377	11	38	theme	therapeutic	1737:1747	arg1	agent					1749:1753	a therapeutic agent	1735:1753	a therapeutic agent for fulminant hepatitis	1735:1777	Thus, a potent inhibitor of SphK1 could potentially be a therapeutic agent for fulminant hepatitis.
33566377	3	39	theme	SphK1	417:421	arg1	role					409:412	the role	405:412	the role of SphK1 in D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced ALF, which is a well-established experimental mouse model that mimics the fulminant hepatitis	405:574	This study examines the role of SphK1 in D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced ALF, which is a well-established experimental mouse model that mimics the fulminant hepatitis.
33566377	0	40	theme	SphK1	26:30	arg1	inhibition					12:21	inhibition	12:21	inhibition	12:21	Deletion or inhibition of SphK1 mitigates fulminant hepatic failure by suppressing TNFα-dependent inflammation and apoptosis.
33566377	0	40	theme	SphK1	26:30	arg1	Deletion					0:7	Deletion	0:7	Deletion	0:7	Deletion or inhibition of SphK1 mitigates fulminant hepatic failure by suppressing TNFα-dependent inflammation and apoptosis.
33566377	7	41	theme	infiltrating	1144:1155	arg1	cells					1164:1168	bone marrow-derived infiltrating immune cells	1124:1168	bone marrow-derived infiltrating immune cells	1124:1168	Adoptive transfer experiments indicated that SphK1 in bone marrow-derived infiltrating immune cells but not in host liver-resident cells, contribute to the development of ALF.
33566377	8	42	theme	TNFα	1273:1276	arg1	production					1278:1287	LPS-induced TNFα production	1261:1287	LPS-induced TNFα production	1261:1287	Interestingly, LPS-induced TNFα production was drastically suppressed in SphK1-deleted macrophages, whereas IL-10 expression was markedly enhanced, suggesting a switch to the anti-inflammatory phenotype.
33566377	3	43	theme	fulminant	556:564	arg1	hepatitis					566:574	the fulminant hepatitis	552:574	the fulminant hepatitis	552:574	This study examines the role of SphK1 in D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced ALF, which is a well-established experimental mouse model that mimics the fulminant hepatitis.
33566377	5	44	theme	hepatic	825:831	arg1	activation					833:842	treatment-induced hepatic activation	807:842	treatment-induced hepatic activation of NF-κB and JNK in wild-type and SphK2-/- mice	807:890	Whereas GalN/LPS treatment-induced hepatic activation of NF-κB and JNK in wild-type and SphK2-/- mice, these signaling pathways were reduced in SphK1-/- mice.
33566377	0	45	theme	hepatic	52:58	arg1	failure					60:66	fulminant hepatic failure	42:66	fulminant hepatic failure	42:66	Deletion or inhibition of SphK1 mitigates fulminant hepatic failure by suppressing TNFα-dependent inflammation and apoptosis.
33566377	7	46	from	SphK1	1115:1119	arg1	cells					1164:1168	bone marrow-derived infiltrating immune cells	1124:1168	bone marrow-derived infiltrating immune cells	1124:1168	Adoptive transfer experiments indicated that SphK1 in bone marrow-derived infiltrating immune cells but not in host liver-resident cells, contribute to the development of ALF.
33566377	7	46	from	SphK1	1115:1119	arg1	cells					1201:1205	host liver-resident cells	1181:1205	host liver-resident cells	1181:1205	Adoptive transfer experiments indicated that SphK1 in bone marrow-derived infiltrating immune cells but not in host liver-resident cells, contribute to the development of ALF.
33566377	7	47	link	marrow-derived	1129:1142	arg1	cells					1164:1168	bone marrow-derived infiltrating immune cells	1124:1168	bone marrow-derived infiltrating immune cells	1124:1168	Adoptive transfer experiments indicated that SphK1 in bone marrow-derived infiltrating immune cells but not in host liver-resident cells, contribute to the development of ALF.
33566377	4	48	theme	liver	671:675	arg1	damage					677:682	GalN/LPS-induced liver damage	654:682	GalN/LPS-induced liver damage	654:682	Here we report that deletion of SphK1, but not SphK2, dramatically decreased GalN/LPS-induced liver damage, hepatic apoptosis, serum alanine aminotransferase levels, and mortality rate compared to wild-type mice.
33566377	0	49	theme	fulminant	42:50	arg1	failure					60:66	fulminant hepatic failure	42:66	fulminant hepatic failure	42:66	Deletion or inhibition of SphK1 mitigates fulminant hepatic failure by suppressing TNFα-dependent inflammation and apoptosis.
33566377	1	50	theme	liver	132:136	arg1	failure					138:144	Acute liver failure	126:144	Acute liver failure (ALF)	126:150	Acute liver failure (ALF) causes severe liver dysfunction that can lead to multi-organ failure and death.
33566377	1	50	theme	liver	132:136	arg1	ALF					147:149	ALF	147:149	ALF	147:149	Acute liver failure (ALF) causes severe liver dysfunction that can lead to multi-organ failure and death.
33566377	7	51	theme	immune	1157:1162	arg1	cells					1164:1168	bone marrow-derived infiltrating immune cells	1124:1168	bone marrow-derived infiltrating immune cells	1124:1168	Adoptive transfer experiments indicated that SphK1 in bone marrow-derived infiltrating immune cells but not in host liver-resident cells, contribute to the development of ALF.
33566377	11	52	theme	SphK1	1708:1712	arg1	agent					1749:1753	a therapeutic agent	1735:1753	a therapeutic agent for fulminant hepatitis	1735:1777	Thus, a potent inhibitor of SphK1 could potentially be a therapeutic agent for fulminant hepatitis.
33566377	11	52	theme	SphK1	1708:1712	arg1	inhibitor					1695:1703	a potent inhibitor	1686:1703	a potent inhibitor of SphK1	1686:1712	Thus, a potent inhibitor of SphK1 could potentially be a therapeutic agent for fulminant hepatitis.
33566377	2	53	theme	sphingosine	262:272	arg1	kinase					274:279	sphingosine kinase 1	262:281	sphingosine kinase 1 (SphK1)	262:289	Previous studies suggest that sphingosine kinase 1 (SphK1) protects against hepatocyte injury, yet not much is still known about its involvement in ALF.
33566377	2	53	theme	sphingosine	262:272	arg1	SphK1					284:288	SphK1	284:288	SphK1	284:288	Previous studies suggest that sphingosine kinase 1 (SphK1) protects against hepatocyte injury, yet not much is still known about its involvement in ALF.
33566377	4	54	theme	mortality	747:755	arg1	rate					757:760	mortality rate	747:760	mortality rate	747:760	Here we report that deletion of SphK1, but not SphK2, dramatically decreased GalN/LPS-induced liver damage, hepatic apoptosis, serum alanine aminotransferase levels, and mortality rate compared to wild-type mice.
33566377	2	55	from	involvement	365:375	arg1	ALF					380:382	ALF	380:382	ALF	380:382	Previous studies suggest that sphingosine kinase 1 (SphK1) protects against hepatocyte injury, yet not much is still known about its involvement in ALF.
33566377	5	56	from	activation	833:842	arg1	mice					887:890	wild-type and SphK2-/- mice	864:890	wild-type and SphK2-/- mice	864:890	Whereas GalN/LPS treatment-induced hepatic activation of NF-κB and JNK in wild-type and SphK2-/- mice, these signaling pathways were reduced in SphK1-/- mice.
33566377	4	57	theme	serum	704:708	arg1	levels					735:740	serum alanine aminotransferase levels	704:740	serum alanine aminotransferase levels	704:740	Here we report that deletion of SphK1, but not SphK2, dramatically decreased GalN/LPS-induced liver damage, hepatic apoptosis, serum alanine aminotransferase levels, and mortality rate compared to wild-type mice.
33566377	9	58	with	treatment	1459:1467	arg1	inhibitor					1491:1499	a specific SphK1 inhibitor	1474:1499	a specific SphK1 inhibitor	1474:1499	Finally, treatment with a specific SphK1 inhibitor ameliorated inflammation and protected mice from ALF.
33566377	5	59	theme	SphK2-/-	878:885	arg1	mice					887:890	wild-type and SphK2-/- mice	864:890	wild-type and SphK2-/- mice	864:890	Whereas GalN/LPS treatment-induced hepatic activation of NF-κB and JNK in wild-type and SphK2-/- mice, these signaling pathways were reduced in SphK1-/- mice.
33566377	8	60	theme	IL-10	1354:1358	arg1	expression					1360:1369	IL-10 expression	1354:1369	IL-10 expression	1354:1369	Interestingly, LPS-induced TNFα production was drastically suppressed in SphK1-deleted macrophages, whereas IL-10 expression was markedly enhanced, suggesting a switch to the anti-inflammatory phenotype.
33566377	6	61	theme	SphK1-/-	980:987	arg1	mice					989:992	SphK1-/- mice	980:992	SphK1-/- mice	980:992	Moreover, repression of ALF in SphK1-/- mice correlated with decreased expression of the pro-inflammatory cytokine TNFα.
33566377	8	62	theme	LPS-induced	1261:1271	arg1	production					1278:1287	LPS-induced TNFα production	1261:1287	LPS-induced TNFα production	1261:1287	Interestingly, LPS-induced TNFα production was drastically suppressed in SphK1-deleted macrophages, whereas IL-10 expression was markedly enhanced, suggesting a switch to the anti-inflammatory phenotype.
33566377	9	63	theme	specific	1476:1483	arg1	inhibitor					1491:1499	a specific SphK1 inhibitor	1474:1499	a specific SphK1 inhibitor	1474:1499	Finally, treatment with a specific SphK1 inhibitor ameliorated inflammation and protected mice from ALF.
33566377	3	64	theme	-induced	473:480	arg1	model					534:538	a well-established experimental mouse model	496:538	a well-established experimental mouse model that mimics the fulminant hepatitis	496:574	This study examines the role of SphK1 in D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced ALF, which is a well-established experimental mouse model that mimics the fulminant hepatitis.
33566377	3	64	theme	-induced	473:480	arg1	ALF					482:484	D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced ALF	426:484	D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced ALF	426:484	This study examines the role of SphK1 in D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced ALF, which is a well-established experimental mouse model that mimics the fulminant hepatitis.
33566377	6	65	theme	pro-inflammatory	1038:1053	arg1	cytokine					1055:1062	the pro-inflammatory cytokine	1034:1062	the pro-inflammatory cytokine TNFα	1034:1067	Moreover, repression of ALF in SphK1-/- mice correlated with decreased expression of the pro-inflammatory cytokine TNFα.
33566377	11	66	theme	fulminant	1759:1767	arg1	hepatitis					1769:1777	fulminant hepatitis	1759:1777	fulminant hepatitis	1759:1777	Thus, a potent inhibitor of SphK1 could potentially be a therapeutic agent for fulminant hepatitis.
33566377	3	67	from	role	409:412	arg1	model					534:538	a well-established experimental mouse model	496:538	a well-established experimental mouse model that mimics the fulminant hepatitis	496:574	This study examines the role of SphK1 in D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced ALF, which is a well-established experimental mouse model that mimics the fulminant hepatitis.
33566377	3	67	from	role	409:412	arg1	ALF					482:484	D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced ALF	426:484	D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced ALF	426:484	This study examines the role of SphK1 in D-galactosamine (GalN)/lipopolysaccharide (LPS)-induced ALF, which is a well-established experimental mouse model that mimics the fulminant hepatitis.
33566377	10	68	theme	SphK1	1659:1663	arg1	inhibition					1633:1642	inhibition	1633:1642	inhibition	1633:1642	Our findings suggest that SphK1 regulates TNFα secretion from macrophages and inhibition or deletion of SphK1 mitigated ALF.
33566377	10	68	theme	SphK1	1659:1663	arg1	deletion					1647:1654	deletion	1647:1654	deletion of SphK1	1647:1663	Our findings suggest that SphK1 regulates TNFα secretion from macrophages and inhibition or deletion of SphK1 mitigated ALF.
33566377	6	69	theme	ALF	973:975	arg1	repression					959:968	repression	959:968	repression of ALF in SphK1-/- mice	959:992	Moreover, repression of ALF in SphK1-/- mice correlated with decreased expression of the pro-inflammatory cytokine TNFα.
34884912	2	0	theme	enzymatic	587:595	arg1	analyses					621:628	enzymatic, isotopic and proteomic analyses	587:628	enzymatic, isotopic and proteomic analyses	587:628	An intraperitoneal injection of extended-release recombinant bovine growth hormone (rbGH, Posilac®) was used in gilthead sea bream fingerlings and juveniles to analyse the metabolic response of liver and red and white muscles by enzymatic, isotopic and proteomic analyses.
34884912	2	1	used	used	462:465	arg2	injection					377:385	An intraperitoneal injection	358:385	An intraperitoneal injection of extended-release recombinant bovine growth hormone (rbGH, Posilac®)	358:456	An intraperitoneal injection of extended-release recombinant bovine growth hormone (rbGH, Posilac®) was used in gilthead sea bream fingerlings and juveniles to analyse the metabolic response of liver and red and white muscles by enzymatic, isotopic and proteomic analyses.
34884912	9	2	theme	antioxidant	1599:1609	arg1	pathways					1611:1618	antioxidant pathways	1599:1618	antioxidant pathways that overall resulted in enhanced somatic growth	1599:1667	There was a fuel switch from carbohydrates to lipids with compensatory changes in antioxidant pathways that overall resulted in enhanced somatic growth.
34884912	2	3	theme	muscles	576:582	arg1	response					540:547	the metabolic response	526:547	the metabolic response of liver and red and white muscles	526:582	An intraperitoneal injection of extended-release recombinant bovine growth hormone (rbGH, Posilac®) was used in gilthead sea bream fingerlings and juveniles to analyse the metabolic response of liver and red and white muscles by enzymatic, isotopic and proteomic analyses.
34884912	9	4	with	lipids	1563:1568	arg1	changes					1588:1594	compensatory changes	1575:1594	compensatory changes in antioxidant pathways that overall resulted in enhanced somatic growth	1575:1667	There was a fuel switch from carbohydrates to lipids with compensatory changes in antioxidant pathways that overall resulted in enhanced somatic growth.
34884912	2	5	theme	proteomic	611:619	arg1	analyses					621:628	enzymatic, isotopic and proteomic analyses	587:628	enzymatic, isotopic and proteomic analyses	587:628	An intraperitoneal injection of extended-release recombinant bovine growth hormone (rbGH, Posilac®) was used in gilthead sea bream fingerlings and juveniles to analyse the metabolic response of liver and red and white muscles by enzymatic, isotopic and proteomic analyses.
34884912	0	6	theme	Recombinant	0:10	arg1	Remodelling					52:62	Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling	0:62	Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling	0:62	Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling Enhances Growth of Gilthead Sea-Bream (Sparus aurata): Insights from Stable Isotopes Composition and Proteomics.
34884912	5	7	from	recycling	969:977	arg1	recycling					933:941	the recycling	929:941	the recycling of proteins	929:953	The isotopic analysis of muscles showed a decrease in the recycling of proteins and a greater recycling of lipids and glycogen in the rBGH groups, which favoured a protein sparing effect.
34884912	5	7	from	recycling	969:977	arg1	groups					1014:1019	the rBGH groups	1005:1019	the rBGH groups	1005:1019	The isotopic analysis of muscles showed a decrease in the recycling of proteins and a greater recycling of lipids and glycogen in the rBGH groups, which favoured a protein sparing effect.
34884912	2	8	theme	isotopic	598:605	arg1	analyses					621:628	enzymatic, isotopic and proteomic analyses	587:628	enzymatic, isotopic and proteomic analyses	587:628	An intraperitoneal injection of extended-release recombinant bovine growth hormone (rbGH, Posilac®) was used in gilthead sea bream fingerlings and juveniles to analyse the metabolic response of liver and red and white muscles by enzymatic, isotopic and proteomic analyses.
34884912	3	9	theme	higher	749:754	arg1	use					762:764	higher lipid use	749:764	higher lipid use	749:764	GH-induced lipolysis and glycogenolysis were reflected in liver composition, and metabolic and redox enzymes reported higher lipid use and lower protein oxidation.
34884912	1	10	theme	insulin-like	196:207	arg1	factors					216:222	insulin-like growth factors	196:222	insulin-like growth factors (GH/IGF axis)	196:236	Growth hormone and insulin-like growth factors (GH/IGF axis) regulate somatic growth in mammals and fish, although their action on metabolism is not fully understood in the latter.
34884912	1	10	theme	insulin-like	196:207	arg1	axis					232:235	GH/IGF axis	225:235	GH/IGF axis	225:235	Growth hormone and insulin-like growth factors (GH/IGF axis) regulate somatic growth in mammals and fish, although their action on metabolism is not fully understood in the latter.
34884912	6	11	theme	white	1111:1115	arg1	muscle					1117:1122	white muscle	1111:1122	white muscle	1111:1122	The protein synthesis capacity (RNA/protein) of white muscle increased, while cytochrome-c-oxidase (COX) protein expression decreased in rBGH group.
34884912	2	12	theme	metabolic	530:538	arg1	response					540:547	the metabolic response	526:547	the metabolic response of liver and red and white muscles	526:582	An intraperitoneal injection of extended-release recombinant bovine growth hormone (rbGH, Posilac®) was used in gilthead sea bream fingerlings and juveniles to analyse the metabolic response of liver and red and white muscles by enzymatic, isotopic and proteomic analyses.
34884912	3	13	theme	lower	770:774	arg1	oxidation					784:792	lower protein oxidation	770:792	lower protein oxidation	770:792	GH-induced lipolysis and glycogenolysis were reflected in liver composition, and metabolic and redox enzymes reported higher lipid use and lower protein oxidation.
34884912	0	14	theme	Stable	133:138	arg1	Isotopes					140:147	Stable Isotopes Composition and Proteomics	133:174	Stable Isotopes Composition and Proteomics	133:174	Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling Enhances Growth of Gilthead Sea-Bream (Sparus aurata): Insights from Stable Isotopes Composition and Proteomics.
34884912	0	14	theme	Stable	133:138	arg1	Proteomics					165:174	Proteomics	165:174	Proteomics	165:174	Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling Enhances Growth of Gilthead Sea-Bream (Sparus aurata): Insights from Stable Isotopes Composition and Proteomics.
34884912	0	14	theme	Stable	133:138	arg1	Composition					149:159	Composition	149:159	Composition	149:159	Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling Enhances Growth of Gilthead Sea-Bream (Sparus aurata): Insights from Stable Isotopes Composition and Proteomics.
34884912	5	15	theme	sparing	1047:1053	arg1	effect					1055:1060	a protein sparing effect	1037:1060	a protein sparing effect	1037:1060	The isotopic analysis of muscles showed a decrease in the recycling of proteins and a greater recycling of lipids and glycogen in the rBGH groups, which favoured a protein sparing effect.
34884912	1	16	theme	GH/IGF	225:230	arg1	factors					216:222	insulin-like growth factors	196:222	insulin-like growth factors (GH/IGF axis)	196:236	Growth hormone and insulin-like growth factors (GH/IGF axis) regulate somatic growth in mammals and fish, although their action on metabolism is not fully understood in the latter.
34884912	1	16	theme	GH/IGF	225:230	arg1	axis					232:235	GH/IGF axis	225:235	GH/IGF axis	225:235	Growth hormone and insulin-like growth factors (GH/IGF axis) regulate somatic growth in mammals and fish, although their action on metabolism is not fully understood in the latter.
34884912	7	17	theme	metabolic	1315:1323	arg1	processes					1325:1333	carbohydrate metabolic processes	1302:1333	carbohydrate metabolic processes	1302:1333	Proteomic analysis of white muscle revealed only downregulation of 8 proteins, related to carbohydrate metabolic processes.
34884912	5	18	theme	isotopic	879:886	arg1	analysis					888:895	The isotopic analysis	875:895	The isotopic analysis of muscles	875:906	The isotopic analysis of muscles showed a decrease in the recycling of proteins and a greater recycling of lipids and glycogen in the rBGH groups, which favoured a protein sparing effect.
34884912	2	19	theme	sea	479:481	arg1	bream					483:487	gilthead sea bream fingerlings and juveniles	470:513	bream	483:487	An intraperitoneal injection of extended-release recombinant bovine growth hormone (rbGH, Posilac®) was used in gilthead sea bream fingerlings and juveniles to analyse the metabolic response of liver and red and white muscles by enzymatic, isotopic and proteomic analyses.
34884912	0	20	theme	Sea-Bream	92:100	arg1	Growth					73:78	Growth	73:78	Growth of Gilthead Sea-Bream (Sparus aurata)	73:116	Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling Enhances Growth of Gilthead Sea-Bream (Sparus aurata): Insights from Stable Isotopes Composition and Proteomics.
34884912	0	21	theme	Sparus	103:108	arg1	Sea-Bream					92:100	Gilthead Sea-Bream	83:100	Gilthead Sea-Bream (Sparus aurata)	83:116	Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling Enhances Growth of Gilthead Sea-Bream (Sparus aurata): Insights from Stable Isotopes Composition and Proteomics.
34884912	0	21	theme	Sparus	103:108	arg1	aurata					110:115	Sparus aurata	103:115	Sparus aurata	103:115	Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling Enhances Growth of Gilthead Sea-Bream (Sparus aurata): Insights from Stable Isotopes Composition and Proteomics.
34884912	9	22	from	changes	1588:1594	arg1	pathways					1611:1618	antioxidant pathways	1599:1618	antioxidant pathways that overall resulted in enhanced somatic growth	1599:1667	There was a fuel switch from carbohydrates to lipids with compensatory changes in antioxidant pathways that overall resulted in enhanced somatic growth.
34884912	2	23	theme	extended-release	390:405	arg1	Posilac®					448:455	Posilac®	448:455	Posilac®	448:455	An intraperitoneal injection of extended-release recombinant bovine growth hormone (rbGH, Posilac®) was used in gilthead sea bream fingerlings and juveniles to analyse the metabolic response of liver and red and white muscles by enzymatic, isotopic and proteomic analyses.
34884912	2	23	theme	extended-release	390:405	arg1	hormone					433:439	extended-release recombinant bovine growth hormone	390:439	extended-release recombinant bovine growth hormone (rbGH, Posilac®)	390:456	An intraperitoneal injection of extended-release recombinant bovine growth hormone (rbGH, Posilac®) was used in gilthead sea bream fingerlings and juveniles to analyse the metabolic response of liver and red and white muscles by enzymatic, isotopic and proteomic analyses.
34884912	5	24	theme	greater	961:967	arg1	recycling					969:977	a greater recycling	959:977	a greater recycling of lipids and glycogen	959:1000	The isotopic analysis of muscles showed a decrease in the recycling of proteins and a greater recycling of lipids and glycogen in the rBGH groups, which favoured a protein sparing effect.
34884912	7	25	theme	carbohydrate	1302:1313	arg1	processes					1325:1333	carbohydrate metabolic processes	1302:1333	carbohydrate metabolic processes	1302:1333	Proteomic analysis of white muscle revealed only downregulation of 8 proteins, related to carbohydrate metabolic processes.
34884912	4	26	dep	increased	834:842	arg1	glycogen					844:851	glycogen	844:851	increased glycogen while reducing lipid	834:872	In white and red muscle reserves, rBGH increased glycogen while reducing lipid.
34884912	7	27	theme	related	1291:1297	arg1	proteins					1281:1288	8 proteins	1279:1288	8 proteins	1279:1288	Proteomic analysis of white muscle revealed only downregulation of 8 proteins, related to carbohydrate metabolic processes.
34884912	8	28	theme	sea	1506:1508	arg1	bream					1510:1514	the gilthead sea bream	1493:1514	the gilthead sea bream	1493:1514	The global results corroborated that GH acted by saving dietary proteins for muscle growth mainly by promoting the use of lipids as energy in the muscles of the gilthead sea bream.
34884912	2	29	theme	hormone	433:439	arg1	injection					377:385	An intraperitoneal injection	358:385	An intraperitoneal injection of extended-release recombinant bovine growth hormone (rbGH, Posilac®)	358:456	An intraperitoneal injection of extended-release recombinant bovine growth hormone (rbGH, Posilac®) was used in gilthead sea bream fingerlings and juveniles to analyse the metabolic response of liver and red and white muscles by enzymatic, isotopic and proteomic analyses.
34884912	0	30	theme	Growth	19:24	arg1	Remodelling					52:62	Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling	0:62	Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling	0:62	Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling Enhances Growth of Gilthead Sea-Bream (Sparus aurata): Insights from Stable Isotopes Composition and Proteomics.
34884912	7	31	theme	proteins	1281:1288	arg1	downregulation					1261:1274	only downregulation	1256:1274	only downregulation of 8 proteins, related to carbohydrate metabolic processes	1256:1333	Proteomic analysis of white muscle revealed only downregulation of 8 proteins, related to carbohydrate metabolic processes.
34884912	4	32	theme	red	808:810	arg1	reserves					819:826	white and red muscle reserves	798:826	white and red muscle reserves	798:826	In white and red muscle reserves, rBGH increased glycogen while reducing lipid.
34884912	2	33	theme	bovine	419:424	arg1	Posilac®					448:455	Posilac®	448:455	Posilac®	448:455	An intraperitoneal injection of extended-release recombinant bovine growth hormone (rbGH, Posilac®) was used in gilthead sea bream fingerlings and juveniles to analyse the metabolic response of liver and red and white muscles by enzymatic, isotopic and proteomic analyses.
34884912	2	33	theme	bovine	419:424	arg1	hormone					433:439	extended-release recombinant bovine growth hormone	390:439	extended-release recombinant bovine growth hormone (rbGH, Posilac®)	390:456	An intraperitoneal injection of extended-release recombinant bovine growth hormone (rbGH, Posilac®) was used in gilthead sea bream fingerlings and juveniles to analyse the metabolic response of liver and red and white muscles by enzymatic, isotopic and proteomic analyses.
34884912	0	34	theme	Metabolic	42:50	arg1	Remodelling					52:62	Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling	0:62	Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling	0:62	Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling Enhances Growth of Gilthead Sea-Bream (Sparus aurata): Insights from Stable Isotopes Composition and Proteomics.
34884912	0	35	dep	Enhances	64:71	arg1	Insights					119:126	Insights	119:126	Insights from Stable Isotopes Composition and Proteomics	119:174	Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling Enhances Growth of Gilthead Sea-Bream (Sparus aurata): Insights from Stable Isotopes Composition and Proteomics.
34884912	8	36	theme	saving	1385:1390	arg1	proteins					1400:1407	saving dietary proteins	1385:1407	saving dietary proteins for muscle growth	1385:1425	The global results corroborated that GH acted by saving dietary proteins for muscle growth mainly by promoting the use of lipids as energy in the muscles of the gilthead sea bream.
34884912	5	37	theme	rBGH	1009:1012	arg1	groups					1014:1019	the rBGH groups	1005:1019	the rBGH groups	1005:1019	The isotopic analysis of muscles showed a decrease in the recycling of proteins and a greater recycling of lipids and glycogen in the rBGH groups, which favoured a protein sparing effect.
34884912	7	38	theme	only	1256:1259	arg1	downregulation					1261:1274	only downregulation	1256:1274	only downregulation of 8 proteins, related to carbohydrate metabolic processes	1256:1333	Proteomic analysis of white muscle revealed only downregulation of 8 proteins, related to carbohydrate metabolic processes.
34884912	2	39	dep	bream	483:487	arg1	fingerlings					489:499	fingerlings	489:499	fingerlings	489:499	An intraperitoneal injection of extended-release recombinant bovine growth hormone (rbGH, Posilac®) was used in gilthead sea bream fingerlings and juveniles to analyse the metabolic response of liver and red and white muscles by enzymatic, isotopic and proteomic analyses.
34884912	9	40	theme	compensatory	1575:1586	arg1	changes					1588:1594	compensatory changes	1575:1594	compensatory changes in antioxidant pathways that overall resulted in enhanced somatic growth	1575:1667	There was a fuel switch from carbohydrates to lipids with compensatory changes in antioxidant pathways that overall resulted in enhanced somatic growth.
34884912	7	41	theme	muscle	1240:1245	arg1	analysis					1222:1229	Proteomic analysis	1212:1229	Proteomic analysis of white muscle	1212:1245	Proteomic analysis of white muscle revealed only downregulation of 8 proteins, related to carbohydrate metabolic processes.
34884912	8	42	theme	muscle	1413:1418	arg1	growth					1420:1425	muscle growth	1413:1425	muscle growth	1413:1425	The global results corroborated that GH acted by saving dietary proteins for muscle growth mainly by promoting the use of lipids as energy in the muscles of the gilthead sea bream.
34884912	6	43	theme	protein	1067:1073	arg1	RNA/protein					1095:1105	RNA/protein	1095:1105	RNA/protein	1095:1105	The protein synthesis capacity (RNA/protein) of white muscle increased, while cytochrome-c-oxidase (COX) protein expression decreased in rBGH group.
34884912	6	43	theme	protein	1067:1073	arg1	capacity					1085:1092	The protein synthesis capacity	1063:1092	The protein synthesis capacity (RNA/protein) of white muscle	1063:1122	The protein synthesis capacity (RNA/protein) of white muscle increased, while cytochrome-c-oxidase (COX) protein expression decreased in rBGH group.
34884912	9	44	theme	enhanced	1645:1652	arg1	growth					1662:1667	enhanced somatic growth	1645:1667	enhanced somatic growth	1645:1667	There was a fuel switch from carbohydrates to lipids with compensatory changes in antioxidant pathways that overall resulted in enhanced somatic growth.
34884912	7	45	theme	Proteomic	1212:1220	arg1	analysis					1222:1229	Proteomic analysis	1212:1229	Proteomic analysis of white muscle	1212:1245	Proteomic analysis of white muscle revealed only downregulation of 8 proteins, related to carbohydrate metabolic processes.
34884912	3	46	theme	liver	689:693	arg1	composition					695:705	liver composition	689:705	liver composition	689:705	GH-induced lipolysis and glycogenolysis were reflected in liver composition, and metabolic and redox enzymes reported higher lipid use and lower protein oxidation.
34884912	1	47	from	action	298:303	arg1	metabolism					308:317	metabolism	308:317	metabolism	308:317	Growth hormone and insulin-like growth factors (GH/IGF axis) regulate somatic growth in mammals and fish, although their action on metabolism is not fully understood in the latter.
34884912	2	48	theme	white	570:574	arg1	muscles					576:582	red and white muscles	562:582	red and white muscles	562:582	An intraperitoneal injection of extended-release recombinant bovine growth hormone (rbGH, Posilac®) was used in gilthead sea bream fingerlings and juveniles to analyse the metabolic response of liver and red and white muscles by enzymatic, isotopic and proteomic analyses.
34884912	6	49	theme	cytochrome-c-oxidase	1141:1160	arg1	expression					1176:1185	cytochrome-c-oxidase (COX) protein expression	1141:1185	cytochrome-c-oxidase (COX) protein expression	1141:1185	The protein synthesis capacity (RNA/protein) of white muscle increased, while cytochrome-c-oxidase (COX) protein expression decreased in rBGH group.
34884912	3	50	theme	redox	726:730	arg1	enzymes					732:738	metabolic and redox enzymes	712:738	metabolic and redox enzymes	712:738	GH-induced lipolysis and glycogenolysis were reflected in liver composition, and metabolic and redox enzymes reported higher lipid use and lower protein oxidation.
34884912	1	51	theme	Growth	177:182	arg1	hormone					184:190	Growth hormone	177:190	Growth hormone	177:190	Growth hormone and insulin-like growth factors (GH/IGF axis) regulate somatic growth in mammals and fish, although their action on metabolism is not fully understood in the latter.
34884912	3	52	theme	metabolic	712:720	arg1	enzymes					732:738	metabolic and redox enzymes	712:738	metabolic and redox enzymes	712:738	GH-induced lipolysis and glycogenolysis were reflected in liver composition, and metabolic and redox enzymes reported higher lipid use and lower protein oxidation.
34884912	9	53	theme	somatic	1654:1660	arg1	growth					1662:1667	enhanced somatic growth	1645:1667	enhanced somatic growth	1645:1667	There was a fuel switch from carbohydrates to lipids with compensatory changes in antioxidant pathways that overall resulted in enhanced somatic growth.
34884912	6	54	theme	muscle	1117:1122	arg1	RNA/protein					1095:1105	RNA/protein	1095:1105	RNA/protein	1095:1105	The protein synthesis capacity (RNA/protein) of white muscle increased, while cytochrome-c-oxidase (COX) protein expression decreased in rBGH group.
34884912	6	54	theme	muscle	1117:1122	arg1	capacity					1085:1092	The protein synthesis capacity	1063:1092	The protein synthesis capacity (RNA/protein) of white muscle	1063:1122	The protein synthesis capacity (RNA/protein) of white muscle increased, while cytochrome-c-oxidase (COX) protein expression decreased in rBGH group.
34884912	3	55	theme	lipid	756:760	arg1	use					762:764	higher lipid use	749:764	higher lipid use	749:764	GH-induced lipolysis and glycogenolysis were reflected in liver composition, and metabolic and redox enzymes reported higher lipid use and lower protein oxidation.
34884912	1	56	theme	growth	209:214	arg1	factors					216:222	insulin-like growth factors	196:222	insulin-like growth factors (GH/IGF axis)	196:236	Growth hormone and insulin-like growth factors (GH/IGF axis) regulate somatic growth in mammals and fish, although their action on metabolism is not fully understood in the latter.
34884912	1	56	theme	growth	209:214	arg1	axis					232:235	GH/IGF axis	225:235	GH/IGF axis	225:235	Growth hormone and insulin-like growth factors (GH/IGF axis) regulate somatic growth in mammals and fish, although their action on metabolism is not fully understood in the latter.
34884912	1	57	from	growth	255:260	arg1	fish					277:280	fish	277:280	fish	277:280	Growth hormone and insulin-like growth factors (GH/IGF axis) regulate somatic growth in mammals and fish, although their action on metabolism is not fully understood in the latter.
34884912	1	57	from	growth	255:260	arg1	mammals					265:271	mammals	265:271	mammals	265:271	Growth hormone and insulin-like growth factors (GH/IGF axis) regulate somatic growth in mammals and fish, although their action on metabolism is not fully understood in the latter.
34884912	5	58	theme	protein	1039:1045	arg1	effect					1055:1060	a protein sparing effect	1037:1060	a protein sparing effect	1037:1060	The isotopic analysis of muscles showed a decrease in the recycling of proteins and a greater recycling of lipids and glycogen in the rBGH groups, which favoured a protein sparing effect.
34884912	6	59	theme	rBGH	1200:1203	arg1	group					1205:1209	rBGH group	1200:1209	rBGH group	1200:1209	The protein synthesis capacity (RNA/protein) of white muscle increased, while cytochrome-c-oxidase (COX) protein expression decreased in rBGH group.
34884912	3	60	theme	protein	776:782	arg1	oxidation					784:792	lower protein oxidation	770:792	lower protein oxidation	770:792	GH-induced lipolysis and glycogenolysis were reflected in liver composition, and metabolic and redox enzymes reported higher lipid use and lower protein oxidation.
34884912	5	61	theme	muscles	900:906	arg1	analysis					888:895	The isotopic analysis	875:895	The isotopic analysis of muscles	875:906	The isotopic analysis of muscles showed a decrease in the recycling of proteins and a greater recycling of lipids and glycogen in the rBGH groups, which favoured a protein sparing effect.
34884912	2	62	theme	red	562:564	arg1	muscles					576:582	red and white muscles	562:582	red and white muscles	562:582	An intraperitoneal injection of extended-release recombinant bovine growth hormone (rbGH, Posilac®) was used in gilthead sea bream fingerlings and juveniles to analyse the metabolic response of liver and red and white muscles by enzymatic, isotopic and proteomic analyses.
34884912	2	63	theme	liver	552:556	arg1	response					540:547	the metabolic response	526:547	the metabolic response of liver and red and white muscles	526:582	An intraperitoneal injection of extended-release recombinant bovine growth hormone (rbGH, Posilac®) was used in gilthead sea bream fingerlings and juveniles to analyse the metabolic response of liver and red and white muscles by enzymatic, isotopic and proteomic analyses.
34884912	8	64	theme	lipids	1458:1463	arg1	use					1451:1453	the use	1447:1453	the use of lipids as energy	1447:1473	The global results corroborated that GH acted by saving dietary proteins for muscle growth mainly by promoting the use of lipids as energy in the muscles of the gilthead sea bream.
34884912	6	65	theme	protein	1168:1174	arg1	expression					1176:1185	cytochrome-c-oxidase (COX) protein expression	1141:1185	cytochrome-c-oxidase (COX) protein expression	1141:1185	The protein synthesis capacity (RNA/protein) of white muscle increased, while cytochrome-c-oxidase (COX) protein expression decreased in rBGH group.
34884912	0	66	theme	Gilthead	83:90	arg1	Sea-Bream					92:100	Gilthead Sea-Bream	83:100	Gilthead Sea-Bream (Sparus aurata)	83:116	Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling Enhances Growth of Gilthead Sea-Bream (Sparus aurata): Insights from Stable Isotopes Composition and Proteomics.
34884912	0	66	theme	Gilthead	83:90	arg1	aurata					110:115	Sparus aurata	103:115	Sparus aurata	103:115	Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling Enhances Growth of Gilthead Sea-Bream (Sparus aurata): Insights from Stable Isotopes Composition and Proteomics.
34884912	0	67	dep	Isotopes	140:147	arg1	Isotopes					140:147	Stable Isotopes Composition and Proteomics	133:174	Stable Isotopes Composition and Proteomics	133:174	Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling Enhances Growth of Gilthead Sea-Bream (Sparus aurata): Insights from Stable Isotopes Composition and Proteomics.
34884912	0	67	dep	Isotopes	140:147	arg1	Proteomics					165:174	Proteomics	165:174	Proteomics	165:174	Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling Enhances Growth of Gilthead Sea-Bream (Sparus aurata): Insights from Stable Isotopes Composition and Proteomics.
34884912	0	67	dep	Isotopes	140:147	arg1	Composition					149:159	Composition	149:159	Composition	149:159	Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling Enhances Growth of Gilthead Sea-Bream (Sparus aurata): Insights from Stable Isotopes Composition and Proteomics.
34884912	6	68	theme	COX	1163:1165	arg1	expression					1176:1185	cytochrome-c-oxidase (COX) protein expression	1141:1185	cytochrome-c-oxidase (COX) protein expression	1141:1185	The protein synthesis capacity (RNA/protein) of white muscle increased, while cytochrome-c-oxidase (COX) protein expression decreased in rBGH group.
34884912	2	69	theme	gilthead	470:477	arg1	bream					483:487	gilthead sea bream fingerlings and juveniles	470:513	bream	483:487	An intraperitoneal injection of extended-release recombinant bovine growth hormone (rbGH, Posilac®) was used in gilthead sea bream fingerlings and juveniles to analyse the metabolic response of liver and red and white muscles by enzymatic, isotopic and proteomic analyses.
34884912	5	70	theme	proteins	946:953	arg1	recycling					933:941	the recycling	929:941	the recycling of proteins	929:953	The isotopic analysis of muscles showed a decrease in the recycling of proteins and a greater recycling of lipids and glycogen in the rBGH groups, which favoured a protein sparing effect.
34884912	8	71	theme	gilthead	1497:1504	arg1	bream					1510:1514	the gilthead sea bream	1493:1514	the gilthead sea bream	1493:1514	The global results corroborated that GH acted by saving dietary proteins for muscle growth mainly by promoting the use of lipids as energy in the muscles of the gilthead sea bream.
34884912	0	72	theme	Bovine	12:17	arg1	Remodelling					52:62	Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling	0:62	Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling	0:62	Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling Enhances Growth of Gilthead Sea-Bream (Sparus aurata): Insights from Stable Isotopes Composition and Proteomics.
34884912	5	73	from	decrease	917:924	arg1	recycling					933:941	the recycling	929:941	the recycling of proteins	929:953	The isotopic analysis of muscles showed a decrease in the recycling of proteins and a greater recycling of lipids and glycogen in the rBGH groups, which favoured a protein sparing effect.
34884912	5	73	from	decrease	917:924	arg1	groups					1014:1019	the rBGH groups	1005:1019	the rBGH groups	1005:1019	The isotopic analysis of muscles showed a decrease in the recycling of proteins and a greater recycling of lipids and glycogen in the rBGH groups, which favoured a protein sparing effect.
34884912	8	74	theme	bream	1510:1514	arg1	muscles					1482:1488	the muscles	1478:1488	the muscles of the gilthead sea bream	1478:1514	The global results corroborated that GH acted by saving dietary proteins for muscle growth mainly by promoting the use of lipids as energy in the muscles of the gilthead sea bream.
34884912	8	75	theme	global	1340:1345	arg1	results					1347:1353	The global results	1336:1353	The global results	1336:1353	The global results corroborated that GH acted by saving dietary proteins for muscle growth mainly by promoting the use of lipids as energy in the muscles of the gilthead sea bream.
34884912	2	76	theme	intraperitoneal	361:375	arg1	injection					377:385	An intraperitoneal injection	358:385	An intraperitoneal injection of extended-release recombinant bovine growth hormone (rbGH, Posilac®)	358:456	An intraperitoneal injection of extended-release recombinant bovine growth hormone (rbGH, Posilac®) was used in gilthead sea bream fingerlings and juveniles to analyse the metabolic response of liver and red and white muscles by enzymatic, isotopic and proteomic analyses.
34884912	0	77	theme	Hormone-Induced	26:40	arg1	Remodelling					52:62	Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling	0:62	Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling	0:62	Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling Enhances Growth of Gilthead Sea-Bream (Sparus aurata): Insights from Stable Isotopes Composition and Proteomics.
34884912	4	78	theme	white	798:802	arg1	reserves					819:826	white and red muscle reserves	798:826	white and red muscle reserves	798:826	In white and red muscle reserves, rBGH increased glycogen while reducing lipid.
34884912	9	79	theme	fuel	1529:1532	arg1	switch					1534:1539	a fuel switch	1527:1539	a fuel switch from carbohydrates to lipids with compensatory changes in antioxidant pathways that overall resulted in enhanced somatic growth	1527:1667	There was a fuel switch from carbohydrates to lipids with compensatory changes in antioxidant pathways that overall resulted in enhanced somatic growth.
34884912	3	80	theme	GH-induced	631:640	arg1	lipolysis					642:650	lipolysis	642:650	lipolysis	642:650	GH-induced lipolysis and glycogenolysis were reflected in liver composition, and metabolic and redox enzymes reported higher lipid use and lower protein oxidation.
34884912	2	81	theme	growth	426:431	arg1	Posilac®					448:455	Posilac®	448:455	Posilac®	448:455	An intraperitoneal injection of extended-release recombinant bovine growth hormone (rbGH, Posilac®) was used in gilthead sea bream fingerlings and juveniles to analyse the metabolic response of liver and red and white muscles by enzymatic, isotopic and proteomic analyses.
34884912	2	81	theme	growth	426:431	arg1	hormone					433:439	extended-release recombinant bovine growth hormone	390:439	extended-release recombinant bovine growth hormone (rbGH, Posilac®)	390:456	An intraperitoneal injection of extended-release recombinant bovine growth hormone (rbGH, Posilac®) was used in gilthead sea bream fingerlings and juveniles to analyse the metabolic response of liver and red and white muscles by enzymatic, isotopic and proteomic analyses.
34884912	5	82	theme	glycogen	993:1000	arg1	recycling					969:977	a greater recycling	959:977	a greater recycling of lipids and glycogen	959:1000	The isotopic analysis of muscles showed a decrease in the recycling of proteins and a greater recycling of lipids and glycogen in the rBGH groups, which favoured a protein sparing effect.
34884912	5	82	theme	glycogen	993:1000	arg1	decrease					917:924	a decrease	915:924	a decrease in the recycling of proteins	915:953	The isotopic analysis of muscles showed a decrease in the recycling of proteins and a greater recycling of lipids and glycogen in the rBGH groups, which favoured a protein sparing effect.
34884912	9	83	from	carbohydrates	1546:1558	arg1	switch					1534:1539	a fuel switch	1527:1539	a fuel switch from carbohydrates to lipids with compensatory changes in antioxidant pathways that overall resulted in enhanced somatic growth	1527:1667	There was a fuel switch from carbohydrates to lipids with compensatory changes in antioxidant pathways that overall resulted in enhanced somatic growth.
34884912	2	84	theme	recombinant	407:417	arg1	Posilac®					448:455	Posilac®	448:455	Posilac®	448:455	An intraperitoneal injection of extended-release recombinant bovine growth hormone (rbGH, Posilac®) was used in gilthead sea bream fingerlings and juveniles to analyse the metabolic response of liver and red and white muscles by enzymatic, isotopic and proteomic analyses.
34884912	2	84	theme	recombinant	407:417	arg1	hormone					433:439	extended-release recombinant bovine growth hormone	390:439	extended-release recombinant bovine growth hormone (rbGH, Posilac®)	390:456	An intraperitoneal injection of extended-release recombinant bovine growth hormone (rbGH, Posilac®) was used in gilthead sea bream fingerlings and juveniles to analyse the metabolic response of liver and red and white muscles by enzymatic, isotopic and proteomic analyses.
34884912	4	85	theme	muscle	812:817	arg1	reserves					819:826	white and red muscle reserves	798:826	white and red muscle reserves	798:826	In white and red muscle reserves, rBGH increased glycogen while reducing lipid.
34884912	7	86	theme	white	1234:1238	arg1	muscle					1240:1245	white muscle	1234:1245	white muscle	1234:1245	Proteomic analysis of white muscle revealed only downregulation of 8 proteins, related to carbohydrate metabolic processes.
34884912	0	87	from	Isotopes	140:147	arg1	Insights					119:126	Insights	119:126	Insights from Stable Isotopes Composition and Proteomics	119:174	Recombinant Bovine Growth Hormone-Induced Metabolic Remodelling Enhances Growth of Gilthead Sea-Bream (Sparus aurata): Insights from Stable Isotopes Composition and Proteomics.
34884912	8	88	theme	dietary	1392:1398	arg1	proteins					1400:1407	saving dietary proteins	1385:1407	saving dietary proteins for muscle growth	1385:1425	The global results corroborated that GH acted by saving dietary proteins for muscle growth mainly by promoting the use of lipids as energy in the muscles of the gilthead sea bream.
34884912	5	89	theme	lipids	982:987	arg1	recycling					969:977	a greater recycling	959:977	a greater recycling of lipids and glycogen	959:1000	The isotopic analysis of muscles showed a decrease in the recycling of proteins and a greater recycling of lipids and glycogen in the rBGH groups, which favoured a protein sparing effect.
34884912	5	89	theme	lipids	982:987	arg1	decrease					917:924	a decrease	915:924	a decrease in the recycling of proteins	915:953	The isotopic analysis of muscles showed a decrease in the recycling of proteins and a greater recycling of lipids and glycogen in the rBGH groups, which favoured a protein sparing effect.
34884912	6	90	theme	synthesis	1075:1083	arg1	RNA/protein					1095:1105	RNA/protein	1095:1105	RNA/protein	1095:1105	The protein synthesis capacity (RNA/protein) of white muscle increased, while cytochrome-c-oxidase (COX) protein expression decreased in rBGH group.
34884912	6	90	theme	synthesis	1075:1083	arg1	capacity					1085:1092	The protein synthesis capacity	1063:1092	The protein synthesis capacity (RNA/protein) of white muscle	1063:1122	The protein synthesis capacity (RNA/protein) of white muscle increased, while cytochrome-c-oxidase (COX) protein expression decreased in rBGH group.
34884912	1	91	theme	somatic	247:253	arg1	growth					255:260	somatic growth	247:260	somatic growth in mammals and fish	247:280	Growth hormone and insulin-like growth factors (GH/IGF axis) regulate somatic growth in mammals and fish, although their action on metabolism is not fully understood in the latter.
32816657	10	0	theme	DNA	1343:1345	arg1	content					1320:1326	The G+C content	1312:1326	The G+C content of the genomic DNA	1312:1345	The G+C content of the genomic DNA was 35.7 mol%.
32816657	10	0	theme	DNA	1343:1345	arg1	%					1359:1359	35.7 mol%	1351:1359	35.7 mol%	1351:1359	The G+C content of the genomic DNA was 35.7 mol%.
32816657	2	1	theme	endospore-forming	113:129	arg1	bacterium					154:162	A Gram-stain-positive, endospore-forming, motile and rod-shaped bacterium	90:162	A Gram-stain-positive, endospore-forming, motile and rod-shaped bacterium	90:162	A Gram-stain-positive, endospore-forming, motile and rod-shaped bacterium, designated as strain DSL-17T, was isolated from a tidal sediment of the East China Sea and characterized phylogenetically and phenotypically.
32816657	10	2	theme	G+C	1316:1318	arg1	content					1320:1326	The G+C content	1312:1326	The G+C content of the genomic DNA	1312:1345	The G+C content of the genomic DNA was 35.7 mol%.
32816657	10	2	theme	G+C	1316:1318	arg1	%					1359:1359	35.7 mol%	1351:1359	35.7 mol%	1351:1359	The G+C content of the genomic DNA was 35.7 mol%.
32816657	6	3	theme	sole	954:957	arg1	MK-7					983:986	MK-7	983:986	MK-7	983:986	The sole respiratory quinone was MK-7.
32816657	6	3	theme	sole	954:957	arg1	quinone					971:977	The sole respiratory quinone	950:977	The sole respiratory quinone	950:977	The sole respiratory quinone was MK-7.
32816657	4	4	theme	strain	494:499	arg1	DSL-17T					501:507	strain DSL-17T	494:507	strain DSL-17T	494:507	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSL-17T was related to members of the genus Metabacillus and shared the highest similarity with Metabacillus litoralis SW-211T (98.6 %), followed by Metabacillus halosaccharovorans E33T (97.9 %), Metabacillus crassostreae JSM 100118T (97.7 %), Metabacillus niabensis 4T19T (97.7 %) and Metabacillus malikii NCCP-662T (97.5 %).
32816657	8	5	theme	polar	1073:1077	arg1	diphosphatidylglycerol					1091:1112	diphosphatidylglycerol	1091:1112	diphosphatidylglycerol (DPG)	1091:1118	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), three unidentified glycolipids and six unidentified lipids.
32816657	8	5	theme	polar	1073:1077	arg1	lipids					1079:1084	The polar lipids	1069:1084	The polar lipids	1069:1084	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), three unidentified glycolipids and six unidentified lipids.
32816657	4	6	theme	Phylogenetic	427:438	arg1	analysis					440:447	Phylogenetic analysis	427:447	Phylogenetic analysis based on 16S rRNA gene sequences	427:480	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSL-17T was related to members of the genus Metabacillus and shared the highest similarity with Metabacillus litoralis SW-211T (98.6 %), followed by Metabacillus halosaccharovorans E33T (97.9 %), Metabacillus crassostreae JSM 100118T (97.7 %), Metabacillus niabensis 4T19T (97.7 %) and Metabacillus malikii NCCP-662T (97.5 %).
32816657	4	7	theme	Metabacillus	745:756	arg1	4T19T					768:772	Metabacillus niabensis 4T19T	745:772	Metabacillus niabensis 4T19T (97.7 %)	745:781	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSL-17T was related to members of the genus Metabacillus and shared the highest similarity with Metabacillus litoralis SW-211T (98.6 %), followed by Metabacillus halosaccharovorans E33T (97.9 %), Metabacillus crassostreae JSM 100118T (97.7 %), Metabacillus niabensis 4T19T (97.7 %) and Metabacillus malikii NCCP-662T (97.5 %).
32816657	4	7	theme	Metabacillus	745:756	arg1	%					780:780	97.7 %	775:780	97.7 %	775:780	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSL-17T was related to members of the genus Metabacillus and shared the highest similarity with Metabacillus litoralis SW-211T (98.6 %), followed by Metabacillus halosaccharovorans E33T (97.9 %), Metabacillus crassostreae JSM 100118T (97.7 %), Metabacillus niabensis 4T19T (97.7 %) and Metabacillus malikii NCCP-662T (97.5 %).
32816657	7	8	contain	had	1004:1006	arg1	DSL-17T					996:1002	Strain DSL-17T	989:1002	Strain DSL-17T	989:1002	Strain DSL-17T had a cell-wall peptidoglycan based on meso-diaminopimelic acid.
32816657	7	8	contain	had	1004:1006	arg2	based					1034:1038	a cell-wall peptidoglycan based	1008:1038	a cell-wall peptidoglycan based	1008:1038	Strain DSL-17T had a cell-wall peptidoglycan based on meso-diaminopimelic acid.
32816657	4	9	theme	litoralis	610:618	arg1	SW-211T					620:626	Metabacillus litoralis SW-211T	597:626	Metabacillus litoralis SW-211T	597:626	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSL-17T was related to members of the genus Metabacillus and shared the highest similarity with Metabacillus litoralis SW-211T (98.6 %), followed by Metabacillus halosaccharovorans E33T (97.9 %), Metabacillus crassostreae JSM 100118T (97.7 %), Metabacillus niabensis 4T19T (97.7 %) and Metabacillus malikii NCCP-662T (97.5 %).
32816657	9	10	theme	fatty	1299:1303	arg1	iso-C15 					1223:1230	iso-C15 	1223:1230	iso-C15 	1223:1230	The strain had iso-C15 : 0, anteiso-C15 : 0, iso-C14 : 0, C16 : 0 and iso-C16 : 0 as major fatty acids.
32816657	9	10	theme	fatty	1299:1303	arg1	acids					1305:1309	major fatty acids	1293:1309	major fatty acids	1293:1309	The strain had iso-C15 : 0, anteiso-C15 : 0, iso-C14 : 0, C16 : 0 and iso-C16 : 0 as major fatty acids.
32816657	3	11	theme	optimum	342:348	arg1	16-47 °C					332:339	16-47 °C	332:339	16-47 °C (optimum 37 °C)	332:355	The strain could grow at 16-47 °C (optimum 37 °C), at pH 6.0-10.0 (optimum 6.0) and with 1-7% (w/v) NaCl (optimum 3 %).
32816657	3	11	theme	optimum	342:348	arg1	37 °C					350:354	optimum 37 °C	342:354	optimum 37 °C	342:354	The strain could grow at 16-47 °C (optimum 37 °C), at pH 6.0-10.0 (optimum 6.0) and with 1-7% (w/v) NaCl (optimum 3 %).
32816657	2	12	theme	Gram-stain-positive	92:110	arg1	bacterium					154:162	A Gram-stain-positive, endospore-forming, motile and rod-shaped bacterium	90:162	A Gram-stain-positive, endospore-forming, motile and rod-shaped bacterium	90:162	A Gram-stain-positive, endospore-forming, motile and rod-shaped bacterium, designated as strain DSL-17T, was isolated from a tidal sediment of the East China Sea and characterized phylogenetically and phenotypically.
32816657	5	13	theme	16S	828:830	arg1	similarities					851:862	16S rRNA gene sequence similarities	828:862	16S rRNA gene sequence similarities between strain DSL-17T	828:885	16S rRNA gene sequence similarities between strain DSL-17T and other members of the genus Metabacillus were below 96.6 %.
32816657	12	14	theme	=MCCC	1586:1590	arg1	DSL-17T					1577:1583	DSL-17T	1577:1583	DSL-17T (=MCCC 1K03777T=DSM 109843T)	1577:1612	nov. is proposed The type strain is DSL-17T (=MCCC 1K03777T=DSM 109843T).
32816657	12	14	theme	=MCCC	1586:1590	arg1	109843T					1605:1611	=MCCC 1K03777T=DSM 109843T	1586:1611	=MCCC 1K03777T=DSM 109843T	1586:1611	nov. is proposed The type strain is DSL-17T (=MCCC 1K03777T=DSM 109843T).
32816657	11	15	theme	genus	1469:1473	arg1	Metabacillus					1475:1486	the genus Metabacillus	1465:1486	the genus Metabacillus	1465:1486	The combined genotypic and phenotypic data indicated that strain DSL-17T represents a novel species of the genus Metabacillus, for which the name Metabacillus sediminilitoris sp.
32816657	5	16	theme	rRNA	832:835	arg1	similarities					851:862	16S rRNA gene sequence similarities	828:862	16S rRNA gene sequence similarities between strain DSL-17T	828:885	16S rRNA gene sequence similarities between strain DSL-17T and other members of the genus Metabacillus were below 96.6 %.
32816657	2	17	theme	rod-shaped	143:152	arg1	bacterium					154:162	A Gram-stain-positive, endospore-forming, motile and rod-shaped bacterium	90:162	A Gram-stain-positive, endospore-forming, motile and rod-shaped bacterium	90:162	A Gram-stain-positive, endospore-forming, motile and rod-shaped bacterium, designated as strain DSL-17T, was isolated from a tidal sediment of the East China Sea and characterized phylogenetically and phenotypically.
32816657	5	18	theme	gene	837:840	arg1	similarities					851:862	16S rRNA gene sequence similarities	828:862	16S rRNA gene sequence similarities between strain DSL-17T	828:885	16S rRNA gene sequence similarities between strain DSL-17T and other members of the genus Metabacillus were below 96.6 %.
32816657	4	19	dep	Metabacillus	697:708	arg1	crassostreae					710:721	crassostreae	710:721	crassostreae	710:721	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSL-17T was related to members of the genus Metabacillus and shared the highest similarity with Metabacillus litoralis SW-211T (98.6 %), followed by Metabacillus halosaccharovorans E33T (97.9 %), Metabacillus crassostreae JSM 100118T (97.7 %), Metabacillus niabensis 4T19T (97.7 %) and Metabacillus malikii NCCP-662T (97.5 %).
32816657	4	20	dep	Metabacillus	787:798	arg1	malikii					800:806	malikii	800:806	malikii	800:806	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSL-17T was related to members of the genus Metabacillus and shared the highest similarity with Metabacillus litoralis SW-211T (98.6 %), followed by Metabacillus halosaccharovorans E33T (97.9 %), Metabacillus crassostreae JSM 100118T (97.7 %), Metabacillus niabensis 4T19T (97.7 %) and Metabacillus malikii NCCP-662T (97.5 %).
32816657	8	21	theme	unidentified	1154:1165	arg1	glycolipids					1167:1177	three unidentified glycolipids	1148:1177	three unidentified glycolipids	1148:1177	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), three unidentified glycolipids and six unidentified lipids.
32816657	2	22	theme	Sea	248:250	arg1	sediment					221:228	a tidal sediment	213:228	a tidal sediment of the East China Sea	213:250	A Gram-stain-positive, endospore-forming, motile and rod-shaped bacterium, designated as strain DSL-17T, was isolated from a tidal sediment of the East China Sea and characterized phylogenetically and phenotypically.
32816657	2	23	attach	isolated	199:206	arg2	bacterium					154:162	A Gram-stain-positive, endospore-forming, motile and rod-shaped bacterium	90:162	A Gram-stain-positive, endospore-forming, motile and rod-shaped bacterium	90:162	A Gram-stain-positive, endospore-forming, motile and rod-shaped bacterium, designated as strain DSL-17T, was isolated from a tidal sediment of the East China Sea and characterized phylogenetically and phenotypically.
32816657	2	23	attach	isolated	199:206	arg1	sediment					221:228	a tidal sediment	213:228	a tidal sediment of the East China Sea	213:250	A Gram-stain-positive, endospore-forming, motile and rod-shaped bacterium, designated as strain DSL-17T, was isolated from a tidal sediment of the East China Sea and characterized phylogenetically and phenotypically.
32816657	0	24	theme	sediminilitoris	13:27	arg1	sp					29:30	Metabacillus sediminilitoris sp	0:30	Metabacillus sediminilitoris sp.	0:31	Metabacillus sediminilitoris sp.
32816657	2	25	theme	motile	132:137	arg1	bacterium					154:162	A Gram-stain-positive, endospore-forming, motile and rod-shaped bacterium	90:162	A Gram-stain-positive, endospore-forming, motile and rod-shaped bacterium	90:162	A Gram-stain-positive, endospore-forming, motile and rod-shaped bacterium, designated as strain DSL-17T, was isolated from a tidal sediment of the East China Sea and characterized phylogenetically and phenotypically.
32816657	4	26	dep	Metabacillus	650:661	arg1	halosaccharovorans					663:680	halosaccharovorans	663:680	halosaccharovorans	663:680	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSL-17T was related to members of the genus Metabacillus and shared the highest similarity with Metabacillus litoralis SW-211T (98.6 %), followed by Metabacillus halosaccharovorans E33T (97.9 %), Metabacillus crassostreae JSM 100118T (97.7 %), Metabacillus niabensis 4T19T (97.7 %) and Metabacillus malikii NCCP-662T (97.5 %).
32816657	4	27	theme	gene	467:470	arg1	sequences					472:480	16S rRNA gene sequences	458:480	16S rRNA gene sequences	458:480	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSL-17T was related to members of the genus Metabacillus and shared the highest similarity with Metabacillus litoralis SW-211T (98.6 %), followed by Metabacillus halosaccharovorans E33T (97.9 %), Metabacillus crassostreae JSM 100118T (97.7 %), Metabacillus niabensis 4T19T (97.7 %) and Metabacillus malikii NCCP-662T (97.5 %).
32816657	1	28	theme	tidal	74:78	arg1	sediment					80:87	a tidal sediment	72:87	a tidal sediment	72:87	nov., a marine bacterium isolated from a tidal sediment.
32816657	11	29	theme	Metabacillus	1475:1486	arg1	species					1454:1460	a novel species	1446:1460	a novel species	1446:1460	The combined genotypic and phenotypic data indicated that strain DSL-17T represents a novel species of the genus Metabacillus, for which the name Metabacillus sediminilitoris sp.
32816657	0	30	theme	Metabacillus	0:11	arg1	sp					29:30	Metabacillus sediminilitoris sp	0:30	Metabacillus sediminilitoris sp.	0:31	Metabacillus sediminilitoris sp.
32816657	1	31	attach	isolated	58:65	arg2	bacterium					48:56	a marine bacterium	39:56	a marine bacterium isolated from a tidal sediment	39:87	nov., a marine bacterium isolated from a tidal sediment.
32816657	1	31	attach	isolated	58:65	arg1	sediment					80:87	a tidal sediment	72:87	a tidal sediment	72:87	nov., a marine bacterium isolated from a tidal sediment.
32816657	11	32	theme	Metabacillus	1508:1519	arg1	sp					1537:1538	the name Metabacillus sediminilitoris sp	1499:1538	the name Metabacillus sediminilitoris sp	1499:1538	The combined genotypic and phenotypic data indicated that strain DSL-17T represents a novel species of the genus Metabacillus, for which the name Metabacillus sediminilitoris sp.
32816657	11	33	theme	combined	1366:1373	arg1	data					1400:1403	The combined genotypic and phenotypic data	1362:1403	The combined genotypic and phenotypic data	1362:1403	The combined genotypic and phenotypic data indicated that strain DSL-17T represents a novel species of the genus Metabacillus, for which the name Metabacillus sediminilitoris sp.
32816657	4	34	theme	highest	573:579	arg1	similarity					581:590	the highest similarity	569:590	the highest similarity	569:590	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSL-17T was related to members of the genus Metabacillus and shared the highest similarity with Metabacillus litoralis SW-211T (98.6 %), followed by Metabacillus halosaccharovorans E33T (97.9 %), Metabacillus crassostreae JSM 100118T (97.7 %), Metabacillus niabensis 4T19T (97.7 %) and Metabacillus malikii NCCP-662T (97.5 %).
32816657	7	35	theme	peptidoglycan	1020:1032	arg1	based					1034:1038	a cell-wall peptidoglycan based	1008:1038	a cell-wall peptidoglycan based	1008:1038	Strain DSL-17T had a cell-wall peptidoglycan based on meso-diaminopimelic acid.
32816657	4	36	theme	rRNA	462:465	arg1	sequences					472:480	16S rRNA gene sequences	458:480	16S rRNA gene sequences	458:480	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSL-17T was related to members of the genus Metabacillus and shared the highest similarity with Metabacillus litoralis SW-211T (98.6 %), followed by Metabacillus halosaccharovorans E33T (97.9 %), Metabacillus crassostreae JSM 100118T (97.7 %), Metabacillus niabensis 4T19T (97.7 %) and Metabacillus malikii NCCP-662T (97.5 %).
32816657	5	37	theme	strain	872:877	arg1	DSL-17T					879:885	strain DSL-17T	872:885	strain DSL-17T	872:885	16S rRNA gene sequence similarities between strain DSL-17T and other members of the genus Metabacillus were below 96.6 %.
32816657	4	38	theme	Metabacillus	650:661	arg1	E33T					682:685	Metabacillus halosaccharovorans E33T	650:685	Metabacillus halosaccharovorans E33T (97.9 %)	650:694	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSL-17T was related to members of the genus Metabacillus and shared the highest similarity with Metabacillus litoralis SW-211T (98.6 %), followed by Metabacillus halosaccharovorans E33T (97.9 %), Metabacillus crassostreae JSM 100118T (97.7 %), Metabacillus niabensis 4T19T (97.7 %) and Metabacillus malikii NCCP-662T (97.5 %).
32816657	4	38	theme	Metabacillus	650:661	arg1	%					693:693	97.9 %	688:693	97.9 %	688:693	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSL-17T was related to members of the genus Metabacillus and shared the highest similarity with Metabacillus litoralis SW-211T (98.6 %), followed by Metabacillus halosaccharovorans E33T (97.9 %), Metabacillus crassostreae JSM 100118T (97.7 %), Metabacillus niabensis 4T19T (97.7 %) and Metabacillus malikii NCCP-662T (97.5 %).
32816657	12	39	theme	type	1562:1565	arg1	DSL-17T					1577:1583	DSL-17T	1577:1583	DSL-17T (=MCCC 1K03777T=DSM 109843T)	1577:1612	nov. is proposed The type strain is DSL-17T (=MCCC 1K03777T=DSM 109843T).
32816657	12	39	theme	type	1562:1565	arg1	strain					1567:1572	The type strain	1558:1572	The type strain	1558:1572	nov. is proposed The type strain is DSL-17T (=MCCC 1K03777T=DSM 109843T).
32816657	3	40	theme	w/v	402:404	arg1	NaCl					407:410	1-7% (w/v) NaCl	396:410	1-7% (w/v) NaCl (optimum 3 %)	396:424	The strain could grow at 16-47 °C (optimum 37 °C), at pH 6.0-10.0 (optimum 6.0) and with 1-7% (w/v) NaCl (optimum 3 %).
32816657	3	40	theme	w/v	402:404	arg1	%					423:423	optimum 3 %	413:423	optimum 3 %	413:423	The strain could grow at 16-47 °C (optimum 37 °C), at pH 6.0-10.0 (optimum 6.0) and with 1-7% (w/v) NaCl (optimum 3 %).
32816657	4	41	theme	Metabacillus	787:798	arg1	NCCP-662T					808:816	Metabacillus malikii NCCP-662T	787:816	Metabacillus malikii NCCP-662T (97.5 %)	787:825	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSL-17T was related to members of the genus Metabacillus and shared the highest similarity with Metabacillus litoralis SW-211T (98.6 %), followed by Metabacillus halosaccharovorans E33T (97.9 %), Metabacillus crassostreae JSM 100118T (97.7 %), Metabacillus niabensis 4T19T (97.7 %) and Metabacillus malikii NCCP-662T (97.5 %).
32816657	4	41	theme	Metabacillus	787:798	arg1	%					824:824	97.5 %	819:824	97.5 %	819:824	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSL-17T was related to members of the genus Metabacillus and shared the highest similarity with Metabacillus litoralis SW-211T (98.6 %), followed by Metabacillus halosaccharovorans E33T (97.9 %), Metabacillus crassostreae JSM 100118T (97.7 %), Metabacillus niabensis 4T19T (97.7 %) and Metabacillus malikii NCCP-662T (97.5 %).
32816657	2	42	theme	strain	179:184	arg1	DSL-17T					186:192	strain DSL-17T	179:192	strain DSL-17T	179:192	A Gram-stain-positive, endospore-forming, motile and rod-shaped bacterium, designated as strain DSL-17T, was isolated from a tidal sediment of the East China Sea and characterized phylogenetically and phenotypically.
32816657	11	43	theme	name	1503:1506	arg1	sp					1537:1538	the name Metabacillus sediminilitoris sp	1499:1538	the name Metabacillus sediminilitoris sp	1499:1538	The combined genotypic and phenotypic data indicated that strain DSL-17T represents a novel species of the genus Metabacillus, for which the name Metabacillus sediminilitoris sp.
32816657	8	44	theme	unidentified	1187:1198	arg1	lipids					1200:1205	six unidentified lipids	1183:1205	six unidentified lipids	1183:1205	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), three unidentified glycolipids and six unidentified lipids.
32816657	3	45	theme	optimum	374:380	arg1	pH					361:362	pH 6.0-10.0	361:371	pH 6.0-10.0 (optimum 6.0)	361:385	The strain could grow at 16-47 °C (optimum 37 °C), at pH 6.0-10.0 (optimum 6.0) and with 1-7% (w/v) NaCl (optimum 3 %).
32816657	3	45	theme	optimum	374:380	arg1	6.0					382:384	optimum 6.0	374:384	optimum 6.0	374:384	The strain could grow at 16-47 °C (optimum 37 °C), at pH 6.0-10.0 (optimum 6.0) and with 1-7% (w/v) NaCl (optimum 3 %).
32816657	9	46	dep	iso-C15 	1223:1230	arg1	 0					1271:1272	 0	1271:1272	 0	1271:1272	The strain had iso-C15 : 0, anteiso-C15 : 0, iso-C14 : 0, C16 : 0 and iso-C16 : 0 as major fatty acids.
32816657	9	46	dep	iso-C15 	1223:1230	arg1	 0					1232:1233	 0	1232:1233	 0	1232:1233	The strain had iso-C15 : 0, anteiso-C15 : 0, iso-C14 : 0, C16 : 0 and iso-C16 : 0 as major fatty acids.
32816657	9	46	dep	iso-C15 	1223:1230	arg1	iso-C16 					1278:1285	iso-C16 	1278:1285	iso-C16 	1278:1285	The strain had iso-C15 : 0, anteiso-C15 : 0, iso-C14 : 0, C16 : 0 and iso-C16 : 0 as major fatty acids.
32816657	9	46	dep	iso-C15 	1223:1230	arg1	C16 					1266:1269	C16 	1266:1269	C16 	1266:1269	The strain had iso-C15 : 0, anteiso-C15 : 0, iso-C14 : 0, C16 : 0 and iso-C16 : 0 as major fatty acids.
32816657	9	46	dep	iso-C15 	1223:1230	arg1	 0					1287:1288	 0	1287:1288	 0	1287:1288	The strain had iso-C15 : 0, anteiso-C15 : 0, iso-C14 : 0, C16 : 0 and iso-C16 : 0 as major fatty acids.
32816657	9	46	dep	iso-C15 	1223:1230	arg1	 0					1249:1250	 0	1249:1250	 0	1249:1250	The strain had iso-C15 : 0, anteiso-C15 : 0, iso-C14 : 0, C16 : 0 and iso-C16 : 0 as major fatty acids.
32816657	7	47	theme	meso-diaminopimelic	1043:1061	arg1	acid					1063:1066	meso-diaminopimelic acid	1043:1066	meso-diaminopimelic acid	1043:1066	Strain DSL-17T had a cell-wall peptidoglycan based on meso-diaminopimelic acid.
32816657	4	48	theme	JSM	723:725	arg1	100118T					727:733	Metabacillus crassostreae JSM 100118T	697:733	Metabacillus crassostreae JSM 100118T (97.7 %)	697:742	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSL-17T was related to members of the genus Metabacillus and shared the highest similarity with Metabacillus litoralis SW-211T (98.6 %), followed by Metabacillus halosaccharovorans E33T (97.9 %), Metabacillus crassostreae JSM 100118T (97.7 %), Metabacillus niabensis 4T19T (97.7 %) and Metabacillus malikii NCCP-662T (97.5 %).
32816657	4	48	theme	JSM	723:725	arg1	%					741:741	97.7 %	736:741	97.7 %	736:741	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSL-17T was related to members of the genus Metabacillus and shared the highest similarity with Metabacillus litoralis SW-211T (98.6 %), followed by Metabacillus halosaccharovorans E33T (97.9 %), Metabacillus crassostreae JSM 100118T (97.7 %), Metabacillus niabensis 4T19T (97.7 %) and Metabacillus malikii NCCP-662T (97.5 %).
32816657	3	49	theme	%	399:399	arg1	NaCl					407:410	1-7% (w/v) NaCl	396:410	1-7% (w/v) NaCl (optimum 3 %)	396:424	The strain could grow at 16-47 °C (optimum 37 °C), at pH 6.0-10.0 (optimum 6.0) and with 1-7% (w/v) NaCl (optimum 3 %).
32816657	3	49	theme	%	399:399	arg1	%					423:423	optimum 3 %	413:423	optimum 3 %	413:423	The strain could grow at 16-47 °C (optimum 37 °C), at pH 6.0-10.0 (optimum 6.0) and with 1-7% (w/v) NaCl (optimum 3 %).
32816657	7	50	theme	cell-wall	1010:1018	arg1	based					1034:1038	a cell-wall peptidoglycan based	1008:1038	a cell-wall peptidoglycan based	1008:1038	Strain DSL-17T had a cell-wall peptidoglycan based on meso-diaminopimelic acid.
32816657	2	51	theme	tidal	215:219	arg1	sediment					221:228	a tidal sediment	213:228	a tidal sediment of the East China Sea	213:250	A Gram-stain-positive, endospore-forming, motile and rod-shaped bacterium, designated as strain DSL-17T, was isolated from a tidal sediment of the East China Sea and characterized phylogenetically and phenotypically.
32816657	11	52	theme	phenotypic	1389:1398	arg1	data					1400:1403	The combined genotypic and phenotypic data	1362:1403	The combined genotypic and phenotypic data	1362:1403	The combined genotypic and phenotypic data indicated that strain DSL-17T represents a novel species of the genus Metabacillus, for which the name Metabacillus sediminilitoris sp.
32816657	4	53	theme	Metabacillus	545:556	arg1	members					524:530	members	524:530	members of the genus Metabacillus	524:556	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSL-17T was related to members of the genus Metabacillus and shared the highest similarity with Metabacillus litoralis SW-211T (98.6 %), followed by Metabacillus halosaccharovorans E33T (97.9 %), Metabacillus crassostreae JSM 100118T (97.7 %), Metabacillus niabensis 4T19T (97.7 %) and Metabacillus malikii NCCP-662T (97.5 %).
32816657	7	54	theme	Strain	989:994	arg1	DSL-17T					996:1002	Strain DSL-17T	989:1002	Strain DSL-17T	989:1002	Strain DSL-17T had a cell-wall peptidoglycan based on meso-diaminopimelic acid.
32816657	9	55	contain	had	1219:1221	arg2	iso-C15 					1223:1230	iso-C15 	1223:1230	iso-C15 	1223:1230	The strain had iso-C15 : 0, anteiso-C15 : 0, iso-C14 : 0, C16 : 0 and iso-C16 : 0 as major fatty acids.
32816657	9	55	contain	had	1219:1221	arg1	strain					1212:1217	The strain	1208:1217	The strain	1208:1217	The strain had iso-C15 : 0, anteiso-C15 : 0, iso-C14 : 0, C16 : 0 and iso-C16 : 0 as major fatty acids.
32816657	9	55	contain	had	1219:1221	arg2	acids					1305:1309	major fatty acids	1293:1309	major fatty acids	1293:1309	The strain had iso-C15 : 0, anteiso-C15 : 0, iso-C14 : 0, C16 : 0 and iso-C16 : 0 as major fatty acids.
32816657	3	56	theme	optimum	413:419	arg1	NaCl					407:410	1-7% (w/v) NaCl	396:410	1-7% (w/v) NaCl (optimum 3 %)	396:424	The strain could grow at 16-47 °C (optimum 37 °C), at pH 6.0-10.0 (optimum 6.0) and with 1-7% (w/v) NaCl (optimum 3 %).
32816657	3	56	theme	optimum	413:419	arg1	%					423:423	optimum 3 %	413:423	optimum 3 %	413:423	The strain could grow at 16-47 °C (optimum 37 °C), at pH 6.0-10.0 (optimum 6.0) and with 1-7% (w/v) NaCl (optimum 3 %).
32816657	4	57	dep	Metabacillus	745:756	arg1	niabensis					758:766	niabensis	758:766	niabensis	758:766	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSL-17T was related to members of the genus Metabacillus and shared the highest similarity with Metabacillus litoralis SW-211T (98.6 %), followed by Metabacillus halosaccharovorans E33T (97.9 %), Metabacillus crassostreae JSM 100118T (97.7 %), Metabacillus niabensis 4T19T (97.7 %) and Metabacillus malikii NCCP-662T (97.5 %).
32816657	4	58	theme	16S	458:460	arg1	sequences					472:480	16S rRNA gene sequences	458:480	16S rRNA gene sequences	458:480	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSL-17T was related to members of the genus Metabacillus and shared the highest similarity with Metabacillus litoralis SW-211T (98.6 %), followed by Metabacillus halosaccharovorans E33T (97.9 %), Metabacillus crassostreae JSM 100118T (97.7 %), Metabacillus niabensis 4T19T (97.7 %) and Metabacillus malikii NCCP-662T (97.5 %).
32816657	11	59	theme	sediminilitoris	1521:1535	arg1	sp					1537:1538	the name Metabacillus sediminilitoris sp	1499:1538	the name Metabacillus sediminilitoris sp	1499:1538	The combined genotypic and phenotypic data indicated that strain DSL-17T represents a novel species of the genus Metabacillus, for which the name Metabacillus sediminilitoris sp.
32816657	11	60	theme	genotypic	1375:1383	arg1	data					1400:1403	The combined genotypic and phenotypic data	1362:1403	The combined genotypic and phenotypic data	1362:1403	The combined genotypic and phenotypic data indicated that strain DSL-17T represents a novel species of the genus Metabacillus, for which the name Metabacillus sediminilitoris sp.
32816657	3	61	theme	3 	421:422	arg1	NaCl					407:410	1-7% (w/v) NaCl	396:410	1-7% (w/v) NaCl (optimum 3 %)	396:424	The strain could grow at 16-47 °C (optimum 37 °C), at pH 6.0-10.0 (optimum 6.0) and with 1-7% (w/v) NaCl (optimum 3 %).
32816657	3	61	theme	3 	421:422	arg1	%					423:423	optimum 3 %	413:423	optimum 3 %	413:423	The strain could grow at 16-47 °C (optimum 37 °C), at pH 6.0-10.0 (optimum 6.0) and with 1-7% (w/v) NaCl (optimum 3 %).
32816657	4	62	dep	shared	562:567	arg1	%					634:634	98.6 %	629:634	98.6 %	629:634	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSL-17T was related to members of the genus Metabacillus and shared the highest similarity with Metabacillus litoralis SW-211T (98.6 %), followed by Metabacillus halosaccharovorans E33T (97.9 %), Metabacillus crassostreae JSM 100118T (97.7 %), Metabacillus niabensis 4T19T (97.7 %) and Metabacillus malikii NCCP-662T (97.5 %).
32816657	5	63	theme	genus	912:916	arg1	Metabacillus					918:929	the genus Metabacillus	908:929	the genus Metabacillus	908:929	16S rRNA gene sequence similarities between strain DSL-17T and other members of the genus Metabacillus were below 96.6 %.
32816657	2	64	theme	China	242:246	arg1	Sea					248:250	the East China Sea	233:250	the East China Sea	233:250	A Gram-stain-positive, endospore-forming, motile and rod-shaped bacterium, designated as strain DSL-17T, was isolated from a tidal sediment of the East China Sea and characterized phylogenetically and phenotypically.
32816657	5	65	theme	other	891:895	arg1	members					897:903	other members	891:903	other members of the genus Metabacillus	891:929	16S rRNA gene sequence similarities between strain DSL-17T and other members of the genus Metabacillus were below 96.6 %.
32816657	1	66	dep	bacterium	48:56	arg1	nov.					33:36	nov.	33:36	nov.	33:36	nov., a marine bacterium isolated from a tidal sediment.
32816657	5	67	theme	sequence	842:849	arg1	similarities					851:862	16S rRNA gene sequence similarities	828:862	16S rRNA gene sequence similarities between strain DSL-17T	828:885	16S rRNA gene sequence similarities between strain DSL-17T and other members of the genus Metabacillus were below 96.6 %.
32816657	5	68	theme	Metabacillus	918:929	arg1	similarities					851:862	16S rRNA gene sequence similarities	828:862	16S rRNA gene sequence similarities between strain DSL-17T	828:885	16S rRNA gene sequence similarities between strain DSL-17T and other members of the genus Metabacillus were below 96.6 %.
32816657	5	68	theme	Metabacillus	918:929	arg1	members					897:903	other members	891:903	other members of the genus Metabacillus	891:929	16S rRNA gene sequence similarities between strain DSL-17T and other members of the genus Metabacillus were below 96.6 %.
32816657	2	69	theme	East	237:240	arg1	Sea					248:250	the East China Sea	233:250	the East China Sea	233:250	A Gram-stain-positive, endospore-forming, motile and rod-shaped bacterium, designated as strain DSL-17T, was isolated from a tidal sediment of the East China Sea and characterized phylogenetically and phenotypically.
32816657	11	70	theme	strain	1420:1425	arg1	DSL-17T					1427:1433	strain DSL-17T	1420:1433	strain DSL-17T	1420:1433	The combined genotypic and phenotypic data indicated that strain DSL-17T represents a novel species of the genus Metabacillus, for which the name Metabacillus sediminilitoris sp.
32816657	10	71	theme	35.7 mol	1351:1358	arg1	content					1320:1326	The G+C content	1312:1326	The G+C content of the genomic DNA	1312:1345	The G+C content of the genomic DNA was 35.7 mol%.
32816657	10	71	theme	35.7 mol	1351:1358	arg1	%					1359:1359	35.7 mol%	1351:1359	35.7 mol%	1351:1359	The G+C content of the genomic DNA was 35.7 mol%.
32816657	9	72	theme	major	1293:1297	arg1	iso-C15 					1223:1230	iso-C15 	1223:1230	iso-C15 	1223:1230	The strain had iso-C15 : 0, anteiso-C15 : 0, iso-C14 : 0, C16 : 0 and iso-C16 : 0 as major fatty acids.
32816657	9	72	theme	major	1293:1297	arg1	acids					1305:1309	major fatty acids	1293:1309	major fatty acids	1293:1309	The strain had iso-C15 : 0, anteiso-C15 : 0, iso-C14 : 0, C16 : 0 and iso-C16 : 0 as major fatty acids.
32816657	4	73	theme	Metabacillus	697:708	arg1	100118T					727:733	Metabacillus crassostreae JSM 100118T	697:733	Metabacillus crassostreae JSM 100118T (97.7 %)	697:742	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSL-17T was related to members of the genus Metabacillus and shared the highest similarity with Metabacillus litoralis SW-211T (98.6 %), followed by Metabacillus halosaccharovorans E33T (97.9 %), Metabacillus crassostreae JSM 100118T (97.7 %), Metabacillus niabensis 4T19T (97.7 %) and Metabacillus malikii NCCP-662T (97.5 %).
32816657	4	73	theme	Metabacillus	697:708	arg1	%					741:741	97.7 %	736:741	97.7 %	736:741	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSL-17T was related to members of the genus Metabacillus and shared the highest similarity with Metabacillus litoralis SW-211T (98.6 %), followed by Metabacillus halosaccharovorans E33T (97.9 %), Metabacillus crassostreae JSM 100118T (97.7 %), Metabacillus niabensis 4T19T (97.7 %) and Metabacillus malikii NCCP-662T (97.5 %).
32816657	4	74	theme	genus	539:543	arg1	Metabacillus					545:556	the genus Metabacillus	535:556	the genus Metabacillus	535:556	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSL-17T was related to members of the genus Metabacillus and shared the highest similarity with Metabacillus litoralis SW-211T (98.6 %), followed by Metabacillus halosaccharovorans E33T (97.9 %), Metabacillus crassostreae JSM 100118T (97.7 %), Metabacillus niabensis 4T19T (97.7 %) and Metabacillus malikii NCCP-662T (97.5 %).
32816657	4	75	theme	Metabacillus	597:608	arg1	SW-211T					620:626	Metabacillus litoralis SW-211T	597:626	Metabacillus litoralis SW-211T	597:626	Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSL-17T was related to members of the genus Metabacillus and shared the highest similarity with Metabacillus litoralis SW-211T (98.6 %), followed by Metabacillus halosaccharovorans E33T (97.9 %), Metabacillus crassostreae JSM 100118T (97.7 %), Metabacillus niabensis 4T19T (97.7 %) and Metabacillus malikii NCCP-662T (97.5 %).
32816657	10	76	theme	genomic	1335:1341	arg1	DNA					1343:1345	the genomic DNA	1331:1345	the genomic DNA	1331:1345	The G+C content of the genomic DNA was 35.7 mol%.
32816657	11	77	theme	novel	1448:1452	arg1	species					1454:1460	a novel species	1446:1460	a novel species	1446:1460	The combined genotypic and phenotypic data indicated that strain DSL-17T represents a novel species of the genus Metabacillus, for which the name Metabacillus sediminilitoris sp.
32816657	1	78	theme	marine	41:46	arg1	bacterium					48:56	a marine bacterium	39:56	a marine bacterium isolated from a tidal sediment	39:87	nov., a marine bacterium isolated from a tidal sediment.
32816657	12	79	theme	1K03777T=DSM	1592:1603	arg1	DSL-17T					1577:1583	DSL-17T	1577:1583	DSL-17T (=MCCC 1K03777T=DSM 109843T)	1577:1612	nov. is proposed The type strain is DSL-17T (=MCCC 1K03777T=DSM 109843T).
32816657	12	79	theme	1K03777T=DSM	1592:1603	arg1	109843T					1605:1611	=MCCC 1K03777T=DSM 109843T	1586:1611	=MCCC 1K03777T=DSM 109843T	1586:1611	nov. is proposed The type strain is DSL-17T (=MCCC 1K03777T=DSM 109843T).
32816657	6	80	theme	respiratory	959:969	arg1	MK-7					983:986	MK-7	983:986	MK-7	983:986	The sole respiratory quinone was MK-7.
32816657	6	80	theme	respiratory	959:969	arg1	quinone					971:977	The sole respiratory quinone	950:977	The sole respiratory quinone	950:977	The sole respiratory quinone was MK-7.
32809927	10	0	theme	analysis	1401:1408	arg1	results					1410:1416	analysis results	1401:1416	analysis results of physiological, biochemical and chemotaxonomic data	1401:1470	Based on analysis results of physiological, biochemical and chemotaxonomic data, strain YIM 102482-1T represents a novel species of the genus Gulosibacter, for which the name Gulosibacter macacae sp.
32809927	12	1	theme	=DSM	1643:1646	arg1	2016023T					1665:1672	=DSM 102156T=CCTCC AB 2016023T	1643:1672	=DSM 102156T=CCTCC AB 2016023T	1643:1672	The type strain is YIM 102482-1T(=DSM 102156T=CCTCC AB 2016023T).
32809927	12	1	theme	=DSM	1643:1646	arg1	102482-1T					1633:1641	YIM 102482-1T	1629:1641	YIM 102482-1T(=DSM 102156T=CCTCC AB 2016023T)	1629:1673	The type strain is YIM 102482-1T(=DSM 102156T=CCTCC AB 2016023T).
32809927	5	2	theme	gene	782:785	arg1	sequences					787:795	16S rRNA gene sequences	773:795	16S rRNA gene sequences	773:795	Furthermore, phylogenetic trees based on 16S rRNA gene sequences and genomic sequences demonstrated that strain YIM 102482-1T formed a distinct branch with all type strains of the genus Gulosibacter.
32809927	2	3	attach	isolated	230:237	arg1	faeces					248:253	the faeces	244:253	the faeces of Macaca mulatta	244:271	A novel Gram-stain-positive, aerobic, non-spore-forming, irregular short rod-shaped actinobacterial strain, designated YIM 102482-1T, was isolated from the faeces of Macaca mulatta.
32809927	2	3	attach	isolated	230:237	arg2	strain					192:197	A novel Gram-stain-positive, aerobic, non-spore-forming, irregular short rod-shaped actinobacterial strain	92:197	A novel Gram-stain-positive, aerobic, non-spore-forming, irregular short rod-shaped actinobacterial strain	92:197	A novel Gram-stain-positive, aerobic, non-spore-forming, irregular short rod-shaped actinobacterial strain, designated YIM 102482-1T, was isolated from the faeces of Macaca mulatta.
32809927	5	4	theme	genomic	801:807	arg1	sequences					809:817	genomic sequences	801:817	genomic sequences	801:817	Furthermore, phylogenetic trees based on 16S rRNA gene sequences and genomic sequences demonstrated that strain YIM 102482-1T formed a distinct branch with all type strains of the genus Gulosibacter.
32809927	2	5	theme	Gram-stain-positive	100:118	arg1	strain					192:197	A novel Gram-stain-positive, aerobic, non-spore-forming, irregular short rod-shaped actinobacterial strain	92:197	A novel Gram-stain-positive, aerobic, non-spore-forming, irregular short rod-shaped actinobacterial strain	92:197	A novel Gram-stain-positive, aerobic, non-spore-forming, irregular short rod-shaped actinobacterial strain, designated YIM 102482-1T, was isolated from the faeces of Macaca mulatta.
32809927	10	6	theme	macacae	1580:1586	arg1	sp					1588:1589	the name Gulosibacter macacae sp	1558:1589	the name Gulosibacter macacae sp	1558:1589	Based on analysis results of physiological, biochemical and chemotaxonomic data, strain YIM 102482-1T represents a novel species of the genus Gulosibacter, for which the name Gulosibacter macacae sp.
32809927	7	7	theme	diagnostic	1170:1179	arg1	acids					1189:1193	the diagnostic diamino acids	1166:1193	the diagnostic diamino acids in the cell-wall peptidoglycan	1166:1224	The predominant menaquinone was MK-9, and 2,4-diaminobutyric acid and ornithine were the diagnostic diamino acids in the cell-wall peptidoglycan.
32809927	7	7	theme	diagnostic	1170:1179	arg1	ornithine					1151:1159	ornithine	1151:1159	ornithine	1151:1159	The predominant menaquinone was MK-9, and 2,4-diaminobutyric acid and ornithine were the diagnostic diamino acids in the cell-wall peptidoglycan.
32809927	7	7	theme	diagnostic	1170:1179	arg1	acid					1142:1145	2,4-diaminobutyric acid	1123:1145	2,4-diaminobutyric acid	1123:1145	The predominant menaquinone was MK-9, and 2,4-diaminobutyric acid and ornithine were the diagnostic diamino acids in the cell-wall peptidoglycan.
32809927	2	8	theme	novel	94:98	arg1	strain					192:197	A novel Gram-stain-positive, aerobic, non-spore-forming, irregular short rod-shaped actinobacterial strain	92:197	A novel Gram-stain-positive, aerobic, non-spore-forming, irregular short rod-shaped actinobacterial strain	92:197	A novel Gram-stain-positive, aerobic, non-spore-forming, irregular short rod-shaped actinobacterial strain, designated YIM 102482-1T, was isolated from the faeces of Macaca mulatta.
32809927	3	9	theme	1.0-3.0	356:362	arg1	%					363:363	%	363:363	%	363:363	Strain YIM 102482-1T grew optimally at 30-37 °C, at pH 8.0 and in the presence of 1.0-3.0% (w/v) NaCl.
32809927	6	10	theme	C16	1058:1060	arg1	anteiso-C15					1025:1035	anteiso-C15 : 0, iso-C16	1025:1048	anteiso-C15	1025:1035	The major whole-cell sugars and cellular fatty acids (>10.0 %) were ribose and rhamnose, and anteiso-C15 : 0, iso-C16 : 0 and C16 : 0, respectively.
32809927	10	11	theme	YIM	1480:1482	arg1	102482-1T					1484:1492	strain YIM 102482-1T	1473:1492	strain YIM 102482-1T	1473:1492	Based on analysis results of physiological, biochemical and chemotaxonomic data, strain YIM 102482-1T represents a novel species of the genus Gulosibacter, for which the name Gulosibacter macacae sp.
32809927	5	12	theme	strain	837:842	arg1	102482-1T					848:856	strain YIM 102482-1T	837:856	strain YIM 102482-1T	837:856	Furthermore, phylogenetic trees based on 16S rRNA gene sequences and genomic sequences demonstrated that strain YIM 102482-1T formed a distinct branch with all type strains of the genus Gulosibacter.
32809927	4	13	theme	gene	415:418	arg1	sequences					420:428	16S rRNA gene sequences	406:428	16S rRNA gene sequences	406:428	Similarly, analysis based on 16S rRNA gene sequences showed that strain YIM 102482-1T was a member of the genus Gulosibacter and most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T (96.0 %), respectively.
32809927	12	14	theme	YIM	1629:1631	arg1	strain					1619:1624	The type strain	1610:1624	The type strain	1610:1624	The type strain is YIM 102482-1T(=DSM 102156T=CCTCC AB 2016023T).
32809927	12	14	theme	YIM	1629:1631	arg1	102482-1T					1633:1641	YIM 102482-1T	1629:1641	YIM 102482-1T(=DSM 102156T=CCTCC AB 2016023T)	1629:1673	The type strain is YIM 102482-1T(=DSM 102156T=CCTCC AB 2016023T).
32809927	12	14	theme	YIM	1629:1631	arg1	2016023T					1665:1672	=DSM 102156T=CCTCC AB 2016023T	1643:1672	=DSM 102156T=CCTCC AB 2016023T	1643:1672	The type strain is YIM 102482-1T(=DSM 102156T=CCTCC AB 2016023T).
32809927	7	15	from	acids	1189:1193	arg1	peptidoglycan					1212:1224	the cell-wall peptidoglycan	1198:1224	the cell-wall peptidoglycan	1198:1224	The predominant menaquinone was MK-9, and 2,4-diaminobutyric acid and ornithine were the diagnostic diamino acids in the cell-wall peptidoglycan.
32809927	4	16	theme	related	519:525	arg1	13485T					701:706	most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T	506:706	most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T (96.0 %)	506:715	Similarly, analysis based on 16S rRNA gene sequences showed that strain YIM 102482-1T was a member of the genus Gulosibacter and most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T (96.0 %), respectively.
32809927	4	16	theme	related	519:525	arg1	%					714:714	96.0 %	709:714	96.0 %	709:714	Similarly, analysis based on 16S rRNA gene sequences showed that strain YIM 102482-1T was a member of the genus Gulosibacter and most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T (96.0 %), respectively.
32809927	10	17	theme	strain	1473:1478	arg1	102482-1T					1484:1492	strain YIM 102482-1T	1473:1492	strain YIM 102482-1T	1473:1492	Based on analysis results of physiological, biochemical and chemotaxonomic data, strain YIM 102482-1T represents a novel species of the genus Gulosibacter, for which the name Gulosibacter macacae sp.
32809927	4	18	theme	rRNA	410:413	arg1	sequences					420:428	16S rRNA gene sequences	406:428	16S rRNA gene sequences	406:428	Similarly, analysis based on 16S rRNA gene sequences showed that strain YIM 102482-1T was a member of the genus Gulosibacter and most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T (96.0 %), respectively.
32809927	3	19	dep	%	363:363	arg1	w/v					366:368	w/v	366:368	w/v	366:368	Strain YIM 102482-1T grew optimally at 30-37 °C, at pH 8.0 and in the presence of 1.0-3.0% (w/v) NaCl.
32809927	6	20	theme	fatty	973:977	arg1	acids					979:983	cellular fatty acids	964:983	cellular fatty acids (>10.0 %)	964:993	The major whole-cell sugars and cellular fatty acids (>10.0 %) were ribose and rhamnose, and anteiso-C15 : 0, iso-C16 : 0 and C16 : 0, respectively.
32809927	6	20	theme	fatty	973:977	arg1	%					992:992	>10.0 %	986:992	>10.0 %	986:992	The major whole-cell sugars and cellular fatty acids (>10.0 %) were ribose and rhamnose, and anteiso-C15 : 0, iso-C16 : 0 and C16 : 0, respectively.
32809927	5	21	theme	distinct	867:874	arg1	branch					876:881	a distinct branch	865:881	a distinct branch with all type strains of the genus Gulosibacter	865:929	Furthermore, phylogenetic trees based on 16S rRNA gene sequences and genomic sequences demonstrated that strain YIM 102482-1T formed a distinct branch with all type strains of the genus Gulosibacter.
32809927	5	22	theme	phylogenetic	745:756	arg1	trees					758:762	phylogenetic trees	745:762	phylogenetic trees based on 16S rRNA gene sequences and genomic sequences	745:817	Furthermore, phylogenetic trees based on 16S rRNA gene sequences and genomic sequences demonstrated that strain YIM 102482-1T formed a distinct branch with all type strains of the genus Gulosibacter.
32809927	9	23	theme	DNA	1344:1346	arg1	%					1389:1389	63.0 mol%	1381:1389	63.0 mol%	1381:1389	The DNA G+C content of YIM 102482-1T was 63.0 mol%.
32809927	9	23	theme	DNA	1344:1346	arg1	content					1352:1358	The DNA G+C content	1340:1358	The DNA G+C content of YIM 102482-1T	1340:1375	The DNA G+C content of YIM 102482-1T was 63.0 mol%.
32809927	10	24	theme	Gulosibacter	1534:1545	arg1	species					1513:1519	a novel species	1505:1519	a novel species	1505:1519	Based on analysis results of physiological, biochemical and chemotaxonomic data, strain YIM 102482-1T represents a novel species of the genus Gulosibacter, for which the name Gulosibacter macacae sp.
32809927	12	25	theme	102156T=CCTCC	1648:1660	arg1	2016023T					1665:1672	=DSM 102156T=CCTCC AB 2016023T	1643:1672	=DSM 102156T=CCTCC AB 2016023T	1643:1672	The type strain is YIM 102482-1T(=DSM 102156T=CCTCC AB 2016023T).
32809927	12	25	theme	102156T=CCTCC	1648:1660	arg1	102482-1T					1633:1641	YIM 102482-1T	1629:1641	YIM 102482-1T(=DSM 102156T=CCTCC AB 2016023T)	1629:1673	The type strain is YIM 102482-1T(=DSM 102156T=CCTCC AB 2016023T).
32809927	1	26	theme	Macaca	69:74	arg1	faeces					84:89	Macaca mulatta faeces	69:89	Macaca mulatta faeces	69:89	nov., a novel actinobacterium isolated from Macaca mulatta faeces.
32809927	6	27	theme	cellular	964:971	arg1	acids					979:983	cellular fatty acids	964:983	cellular fatty acids (>10.0 %)	964:993	The major whole-cell sugars and cellular fatty acids (>10.0 %) were ribose and rhamnose, and anteiso-C15 : 0, iso-C16 : 0 and C16 : 0, respectively.
32809927	6	27	theme	cellular	964:971	arg1	%					992:992	>10.0 %	986:992	>10.0 %	986:992	The major whole-cell sugars and cellular fatty acids (>10.0 %) were ribose and rhamnose, and anteiso-C15 : 0, iso-C16 : 0 and C16 : 0, respectively.
32809927	0	28	theme	macacae	13:19	arg1	sp					21:22	Gulosibacter macacae sp	0:22	Gulosibacter macacae sp.	0:23	Gulosibacter macacae sp.
32809927	10	29	theme	biochemical	1436:1446	arg1	data					1467:1470	physiological, biochemical and chemotaxonomic data	1421:1470	physiological, biochemical and chemotaxonomic data	1421:1470	Based on analysis results of physiological, biochemical and chemotaxonomic data, strain YIM 102482-1T represents a novel species of the genus Gulosibacter, for which the name Gulosibacter macacae sp.
32809927	7	30	from	MK-9	1113:1116	arg1	peptidoglycan					1212:1224	the cell-wall peptidoglycan	1198:1224	the cell-wall peptidoglycan	1198:1224	The predominant menaquinone was MK-9, and 2,4-diaminobutyric acid and ornithine were the diagnostic diamino acids in the cell-wall peptidoglycan.
32809927	1	31	theme	mulatta	76:82	arg1	faeces					84:89	Macaca mulatta faeces	69:89	Macaca mulatta faeces	69:89	nov., a novel actinobacterium isolated from Macaca mulatta faeces.
32809927	3	32	theme	NaCl	371:374	arg1	presence					344:351	the presence	340:351	the presence of 1.0-3.0% (w/v) NaCl	340:374	Strain YIM 102482-1T grew optimally at 30-37 °C, at pH 8.0 and in the presence of 1.0-3.0% (w/v) NaCl.
32809927	0	33	theme	Gulosibacter	0:11	arg1	sp					21:22	Gulosibacter macacae sp	0:22	Gulosibacter macacae sp.	0:23	Gulosibacter macacae sp.
32809927	4	34	theme	genus	483:487	arg1	Gulosibacter					489:500	the genus Gulosibacter	479:500	the genus Gulosibacter	479:500	Similarly, analysis based on 16S rRNA gene sequences showed that strain YIM 102482-1T was a member of the genus Gulosibacter and most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T (96.0 %), respectively.
32809927	4	35	theme	KCTC	645:648	arg1	13959T					650:655	KCTC 13959T	645:655	Gulosibacter chungangensis KCTC 13959T (96.4 %)	618:664	Similarly, analysis based on 16S rRNA gene sequences showed that strain YIM 102482-1T was a member of the genus Gulosibacter and most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T (96.0 %), respectively.
32809927	4	35	theme	KCTC	645:648	arg1	%					663:663	96.4 %	658:663	96.4 %	658:663	Similarly, analysis based on 16S rRNA gene sequences showed that strain YIM 102482-1T was a member of the genus Gulosibacter and most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T (96.0 %), respectively.
32809927	7	36	theme	cell-wall	1202:1210	arg1	peptidoglycan					1212:1224	the cell-wall peptidoglycan	1198:1224	the cell-wall peptidoglycan	1198:1224	The predominant menaquinone was MK-9, and 2,4-diaminobutyric acid and ornithine were the diagnostic diamino acids in the cell-wall peptidoglycan.
32809927	8	37	theme	unidentified	1315:1326	arg1	glycolipid					1328:1337	unidentified glycolipid	1315:1337	unidentified glycolipid	1315:1337	The dominant polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and unidentified glycolipid.
32809927	10	38	theme	data	1467:1470	arg1	results					1410:1416	analysis results	1401:1416	analysis results of physiological, biochemical and chemotaxonomic data	1401:1470	Based on analysis results of physiological, biochemical and chemotaxonomic data, strain YIM 102482-1T represents a novel species of the genus Gulosibacter, for which the name Gulosibacter macacae sp.
32809927	6	39	theme	0	1052:1052	arg1	anteiso-C15					1025:1035	anteiso-C15 : 0, iso-C16	1025:1048	anteiso-C15	1025:1035	The major whole-cell sugars and cellular fatty acids (>10.0 %) were ribose and rhamnose, and anteiso-C15 : 0, iso-C16 : 0 and C16 : 0, respectively.
32809927	3	40	theme	Strain	274:279	arg1	102482-1T					285:293	Strain YIM 102482-1T	274:293	Strain YIM 102482-1T	274:293	Strain YIM 102482-1T grew optimally at 30-37 °C, at pH 8.0 and in the presence of 1.0-3.0% (w/v) NaCl.
32809927	7	41	theme	predominant	1085:1095	arg1	MK-9					1113:1116	MK-9	1113:1116	MK-9	1113:1116	The predominant menaquinone was MK-9, and 2,4-diaminobutyric acid and ornithine were the diagnostic diamino acids in the cell-wall peptidoglycan.
32809927	7	41	theme	predominant	1085:1095	arg1	menaquinone					1097:1107	The predominant menaquinone	1081:1107	The predominant menaquinone	1081:1107	The predominant menaquinone was MK-9, and 2,4-diaminobutyric acid and ornithine were the diagnostic diamino acids in the cell-wall peptidoglycan.
32809927	5	42	theme	YIM	844:846	arg1	102482-1T					848:856	strain YIM 102482-1T	837:856	strain YIM 102482-1T	837:856	Furthermore, phylogenetic trees based on 16S rRNA gene sequences and genomic sequences demonstrated that strain YIM 102482-1T formed a distinct branch with all type strains of the genus Gulosibacter.
32809927	6	43	theme	whole-cell	942:951	arg1	ribose					1000:1005	ribose	1000:1005	ribose	1000:1005	The major whole-cell sugars and cellular fatty acids (>10.0 %) were ribose and rhamnose, and anteiso-C15 : 0, iso-C16 : 0 and C16 : 0, respectively.
32809927	6	43	theme	whole-cell	942:951	arg1	sugars					953:958	The major whole-cell sugars	932:958	The major whole-cell sugars	932:958	The major whole-cell sugars and cellular fatty acids (>10.0 %) were ribose and rhamnose, and anteiso-C15 : 0, iso-C16 : 0 and C16 : 0, respectively.
32809927	4	44	dep	Gulosibacter	618:629	arg1	13959T					650:655	KCTC 13959T	645:655	Gulosibacter chungangensis KCTC 13959T (96.4 %)	618:664	Similarly, analysis based on 16S rRNA gene sequences showed that strain YIM 102482-1T was a member of the genus Gulosibacter and most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T (96.0 %), respectively.
32809927	4	44	dep	Gulosibacter	618:629	arg1	%					663:663	96.4 %	658:663	96.4 %	658:663	Similarly, analysis based on 16S rRNA gene sequences showed that strain YIM 102482-1T was a member of the genus Gulosibacter and most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T (96.0 %), respectively.
32809927	4	44	dep	Gulosibacter	618:629	arg1	chungangensis					631:643	Gulosibacter chungangensis KCTC 13959T (96.4 %)	618:664	Gulosibacter chungangensis KCTC 13959T (96.4 %)	618:664	Similarly, analysis based on 16S rRNA gene sequences showed that strain YIM 102482-1T was a member of the genus Gulosibacter and most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T (96.0 %), respectively.
32809927	3	45	theme	YIM	281:283	arg1	102482-1T					285:293	Strain YIM 102482-1T	274:293	Strain YIM 102482-1T	274:293	Strain YIM 102482-1T grew optimally at 30-37 °C, at pH 8.0 and in the presence of 1.0-3.0% (w/v) NaCl.
32809927	6	46	theme	major	936:940	arg1	ribose					1000:1005	ribose	1000:1005	ribose	1000:1005	The major whole-cell sugars and cellular fatty acids (>10.0 %) were ribose and rhamnose, and anteiso-C15 : 0, iso-C16 : 0 and C16 : 0, respectively.
32809927	6	46	theme	major	936:940	arg1	sugars					953:958	The major whole-cell sugars	932:958	The major whole-cell sugars	932:958	The major whole-cell sugars and cellular fatty acids (>10.0 %) were ribose and rhamnose, and anteiso-C15 : 0, iso-C16 : 0 and C16 : 0, respectively.
32809927	4	47	theme	Gulosibacter	489:500	arg1	member					469:474	a member	467:474	a member of the genus Gulosibacter	467:500	Similarly, analysis based on 16S rRNA gene sequences showed that strain YIM 102482-1T was a member of the genus Gulosibacter and most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T (96.0 %), respectively.
32809927	4	47	theme	Gulosibacter	489:500	arg1	13485T					701:706	most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T	506:706	most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T (96.0 %)	506:715	Similarly, analysis based on 16S rRNA gene sequences showed that strain YIM 102482-1T was a member of the genus Gulosibacter and most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T (96.0 %), respectively.
32809927	4	47	theme	Gulosibacter	489:500	arg1	102482-1T					453:461	strain YIM 102482-1T	442:461	strain YIM 102482-1T	442:461	Similarly, analysis based on 16S rRNA gene sequences showed that strain YIM 102482-1T was a member of the genus Gulosibacter and most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T (96.0 %), respectively.
32809927	4	47	theme	Gulosibacter	489:500	arg1	%					714:714	96.0 %	709:714	96.0 %	709:714	Similarly, analysis based on 16S rRNA gene sequences showed that strain YIM 102482-1T was a member of the genus Gulosibacter and most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T (96.0 %), respectively.
32809927	9	48	theme	102482-1T	1367:1375	arg1	%					1389:1389	63.0 mol%	1381:1389	63.0 mol%	1381:1389	The DNA G+C content of YIM 102482-1T was 63.0 mol%.
32809927	9	48	theme	102482-1T	1367:1375	arg1	content					1352:1358	The DNA G+C content	1340:1358	The DNA G+C content of YIM 102482-1T	1340:1375	The DNA G+C content of YIM 102482-1T was 63.0 mol%.
32809927	2	49	theme	actinobacterial	176:190	arg1	strain					192:197	A novel Gram-stain-positive, aerobic, non-spore-forming, irregular short rod-shaped actinobacterial strain	92:197	A novel Gram-stain-positive, aerobic, non-spore-forming, irregular short rod-shaped actinobacterial strain	92:197	A novel Gram-stain-positive, aerobic, non-spore-forming, irregular short rod-shaped actinobacterial strain, designated YIM 102482-1T, was isolated from the faeces of Macaca mulatta.
32809927	10	50	theme	novel	1507:1511	arg1	species					1513:1519	a novel species	1505:1519	a novel species	1505:1519	Based on analysis results of physiological, biochemical and chemotaxonomic data, strain YIM 102482-1T represents a novel species of the genus Gulosibacter, for which the name Gulosibacter macacae sp.
32809927	7	51	theme	2,4-diaminobutyric	1123:1140	arg1	acids					1189:1193	the diagnostic diamino acids	1166:1193	the diagnostic diamino acids in the cell-wall peptidoglycan	1166:1224	The predominant menaquinone was MK-9, and 2,4-diaminobutyric acid and ornithine were the diagnostic diamino acids in the cell-wall peptidoglycan.
32809927	7	51	theme	2,4-diaminobutyric	1123:1140	arg1	ornithine					1151:1159	ornithine	1151:1159	ornithine	1151:1159	The predominant menaquinone was MK-9, and 2,4-diaminobutyric acid and ornithine were the diagnostic diamino acids in the cell-wall peptidoglycan.
32809927	7	51	theme	2,4-diaminobutyric	1123:1140	arg1	acid					1142:1145	2,4-diaminobutyric acid	1123:1145	2,4-diaminobutyric acid	1123:1145	The predominant menaquinone was MK-9, and 2,4-diaminobutyric acid and ornithine were the diagnostic diamino acids in the cell-wall peptidoglycan.
32809927	5	52	with	branch	876:881	arg1	strains					897:903	all type strains	888:903	all type strains of the genus Gulosibacter	888:929	Furthermore, phylogenetic trees based on 16S rRNA gene sequences and genomic sequences demonstrated that strain YIM 102482-1T formed a distinct branch with all type strains of the genus Gulosibacter.
32809927	2	53	dep	Gram-stain-positive	100:118	arg1	non-spore-forming					130:146	non-spore-forming	130:146	non-spore-forming	130:146	A novel Gram-stain-positive, aerobic, non-spore-forming, irregular short rod-shaped actinobacterial strain, designated YIM 102482-1T, was isolated from the faeces of Macaca mulatta.
32809927	2	53	dep	Gram-stain-positive	100:118	arg1	aerobic					121:127	aerobic	121:127	aerobic	121:127	A novel Gram-stain-positive, aerobic, non-spore-forming, irregular short rod-shaped actinobacterial strain, designated YIM 102482-1T, was isolated from the faeces of Macaca mulatta.
32809927	2	53	dep	Gram-stain-positive	100:118	arg1	irregular					149:157	irregular	149:157	irregular	149:157	A novel Gram-stain-positive, aerobic, non-spore-forming, irregular short rod-shaped actinobacterial strain, designated YIM 102482-1T, was isolated from the faeces of Macaca mulatta.
32809927	2	54	theme	rod-shaped	165:174	arg1	strain					192:197	A novel Gram-stain-positive, aerobic, non-spore-forming, irregular short rod-shaped actinobacterial strain	92:197	A novel Gram-stain-positive, aerobic, non-spore-forming, irregular short rod-shaped actinobacterial strain	92:197	A novel Gram-stain-positive, aerobic, non-spore-forming, irregular short rod-shaped actinobacterial strain, designated YIM 102482-1T, was isolated from the faeces of Macaca mulatta.
32809927	8	55	theme	polar	1240:1244	arg1	lipids					1246:1251	The dominant polar lipids	1227:1251	The dominant polar lipids	1227:1251	The dominant polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and unidentified glycolipid.
32809927	10	56	theme	physiological	1421:1433	arg1	data					1467:1470	physiological, biochemical and chemotaxonomic data	1421:1470	physiological, biochemical and chemotaxonomic data	1421:1470	Based on analysis results of physiological, biochemical and chemotaxonomic data, strain YIM 102482-1T represents a novel species of the genus Gulosibacter, for which the name Gulosibacter macacae sp.
32809927	3	57	theme	%	363:363	arg1	NaCl					371:374	1.0-3.0% (w/v) NaCl	356:374	1.0-3.0% (w/v) NaCl	356:374	Strain YIM 102482-1T grew optimally at 30-37 °C, at pH 8.0 and in the presence of 1.0-3.0% (w/v) NaCl.
32809927	2	58	theme	short	159:163	arg1	strain					192:197	A novel Gram-stain-positive, aerobic, non-spore-forming, irregular short rod-shaped actinobacterial strain	92:197	A novel Gram-stain-positive, aerobic, non-spore-forming, irregular short rod-shaped actinobacterial strain	92:197	A novel Gram-stain-positive, aerobic, non-spore-forming, irregular short rod-shaped actinobacterial strain, designated YIM 102482-1T, was isolated from the faeces of Macaca mulatta.
32809927	5	59	theme	genus	912:916	arg1	Gulosibacter					918:929	the genus Gulosibacter	908:929	the genus Gulosibacter	908:929	Furthermore, phylogenetic trees based on 16S rRNA gene sequences and genomic sequences demonstrated that strain YIM 102482-1T formed a distinct branch with all type strains of the genus Gulosibacter.
32809927	12	60	theme	AB	1662:1663	arg1	2016023T					1665:1672	=DSM 102156T=CCTCC AB 2016023T	1643:1672	=DSM 102156T=CCTCC AB 2016023T	1643:1672	The type strain is YIM 102482-1T(=DSM 102156T=CCTCC AB 2016023T).
32809927	12	60	theme	AB	1662:1663	arg1	102482-1T					1633:1641	YIM 102482-1T	1629:1641	YIM 102482-1T(=DSM 102156T=CCTCC AB 2016023T)	1629:1673	The type strain is YIM 102482-1T(=DSM 102156T=CCTCC AB 2016023T).
32809927	9	61	theme	YIM	1363:1365	arg1	102482-1T					1367:1375	YIM 102482-1T	1363:1375	YIM 102482-1T	1363:1375	The DNA G+C content of YIM 102482-1T was 63.0 mol%.
32809927	4	62	theme	YIM	449:451	arg1	member					469:474	a member	467:474	a member of the genus Gulosibacter	467:500	Similarly, analysis based on 16S rRNA gene sequences showed that strain YIM 102482-1T was a member of the genus Gulosibacter and most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T (96.0 %), respectively.
32809927	4	62	theme	YIM	449:451	arg1	102482-1T					453:461	strain YIM 102482-1T	442:461	strain YIM 102482-1T	442:461	Similarly, analysis based on 16S rRNA gene sequences showed that strain YIM 102482-1T was a member of the genus Gulosibacter and most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T (96.0 %), respectively.
32809927	5	63	theme	Gulosibacter	918:929	arg1	strains					897:903	all type strains	888:903	all type strains of the genus Gulosibacter	888:929	Furthermore, phylogenetic trees based on 16S rRNA gene sequences and genomic sequences demonstrated that strain YIM 102482-1T formed a distinct branch with all type strains of the genus Gulosibacter.
32809927	9	64	theme	G+C	1348:1350	arg1	%					1389:1389	63.0 mol%	1381:1389	63.0 mol%	1381:1389	The DNA G+C content of YIM 102482-1T was 63.0 mol%.
32809927	9	64	theme	G+C	1348:1350	arg1	content					1352:1358	The DNA G+C content	1340:1358	The DNA G+C content of YIM 102482-1T	1340:1375	The DNA G+C content of YIM 102482-1T was 63.0 mol%.
32809927	4	65	theme	16S	406:408	arg1	sequences					420:428	16S rRNA gene sequences	406:428	16S rRNA gene sequences	406:428	Similarly, analysis based on 16S rRNA gene sequences showed that strain YIM 102482-1T was a member of the genus Gulosibacter and most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T (96.0 %), respectively.
32809927	2	66	theme	YIM	211:213	arg1	102482-1T					215:223	YIM 102482-1T	211:223	YIM 102482-1T	211:223	A novel Gram-stain-positive, aerobic, non-spore-forming, irregular short rod-shaped actinobacterial strain, designated YIM 102482-1T, was isolated from the faeces of Macaca mulatta.
32809927	2	67	theme	Macaca	258:263	arg1	mulatta					265:271	Macaca mulatta	258:271	Macaca mulatta	258:271	A novel Gram-stain-positive, aerobic, non-spore-forming, irregular short rod-shaped actinobacterial strain, designated YIM 102482-1T, was isolated from the faeces of Macaca mulatta.
32809927	12	68	theme	type	1614:1617	arg1	strain					1619:1624	The type strain	1610:1624	The type strain	1610:1624	The type strain is YIM 102482-1T(=DSM 102156T=CCTCC AB 2016023T).
32809927	12	68	theme	type	1614:1617	arg1	102482-1T					1633:1641	YIM 102482-1T	1629:1641	YIM 102482-1T(=DSM 102156T=CCTCC AB 2016023T)	1629:1673	The type strain is YIM 102482-1T(=DSM 102156T=CCTCC AB 2016023T).
32809927	8	69	theme	dominant	1231:1238	arg1	lipids					1246:1251	The dominant polar lipids	1227:1251	The dominant polar lipids	1227:1251	The dominant polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and unidentified glycolipid.
32809927	2	70	theme	mulatta	265:271	arg1	faeces					248:253	the faeces	244:253	the faeces of Macaca mulatta	244:271	A novel Gram-stain-positive, aerobic, non-spore-forming, irregular short rod-shaped actinobacterial strain, designated YIM 102482-1T, was isolated from the faeces of Macaca mulatta.
32809927	4	71	theme	molinativorax	683:695	arg1	13485T					701:706	most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T	506:706	most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T (96.0 %)	506:715	Similarly, analysis based on 16S rRNA gene sequences showed that strain YIM 102482-1T was a member of the genus Gulosibacter and most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T (96.0 %), respectively.
32809927	4	71	theme	molinativorax	683:695	arg1	%					714:714	96.0 %	709:714	96.0 %	709:714	Similarly, analysis based on 16S rRNA gene sequences showed that strain YIM 102482-1T was a member of the genus Gulosibacter and most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T (96.0 %), respectively.
32809927	10	72	theme	genus	1528:1532	arg1	Gulosibacter					1534:1545	the genus Gulosibacter	1524:1545	the genus Gulosibacter	1524:1545	Based on analysis results of physiological, biochemical and chemotaxonomic data, strain YIM 102482-1T represents a novel species of the genus Gulosibacter, for which the name Gulosibacter macacae sp.
32809927	4	73	theme	DSM	697:699	arg1	13485T					701:706	most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T	506:706	most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T (96.0 %)	506:715	Similarly, analysis based on 16S rRNA gene sequences showed that strain YIM 102482-1T was a member of the genus Gulosibacter and most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T (96.0 %), respectively.
32809927	4	73	theme	DSM	697:699	arg1	%					714:714	96.0 %	709:714	96.0 %	709:714	Similarly, analysis based on 16S rRNA gene sequences showed that strain YIM 102482-1T was a member of the genus Gulosibacter and most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T (96.0 %), respectively.
32809927	10	74	theme	name	1562:1565	arg1	sp					1588:1589	the name Gulosibacter macacae sp	1558:1589	the name Gulosibacter macacae sp	1558:1589	Based on analysis results of physiological, biochemical and chemotaxonomic data, strain YIM 102482-1T represents a novel species of the genus Gulosibacter, for which the name Gulosibacter macacae sp.
32809927	5	75	theme	type	892:895	arg1	strains					897:903	all type strains	888:903	all type strains of the genus Gulosibacter	888:929	Furthermore, phylogenetic trees based on 16S rRNA gene sequences and genomic sequences demonstrated that strain YIM 102482-1T formed a distinct branch with all type strains of the genus Gulosibacter.
32809927	9	76	theme	mol	1386:1388	arg1	%					1389:1389	63.0 mol%	1381:1389	63.0 mol%	1381:1389	The DNA G+C content of YIM 102482-1T was 63.0 mol%.
32809927	9	76	theme	mol	1386:1388	arg1	content					1352:1358	The DNA G+C content	1340:1358	The DNA G+C content of YIM 102482-1T	1340:1375	The DNA G+C content of YIM 102482-1T was 63.0 mol%.
32809927	5	77	theme	16S	773:775	arg1	sequences					787:795	16S rRNA gene sequences	773:795	16S rRNA gene sequences	773:795	Furthermore, phylogenetic trees based on 16S rRNA gene sequences and genomic sequences demonstrated that strain YIM 102482-1T formed a distinct branch with all type strains of the genus Gulosibacter.
32809927	7	78	theme	diamino	1181:1187	arg1	acids					1189:1193	the diagnostic diamino acids	1166:1193	the diagnostic diamino acids in the cell-wall peptidoglycan	1166:1224	The predominant menaquinone was MK-9, and 2,4-diaminobutyric acid and ornithine were the diagnostic diamino acids in the cell-wall peptidoglycan.
32809927	7	78	theme	diamino	1181:1187	arg1	ornithine					1151:1159	ornithine	1151:1159	ornithine	1151:1159	The predominant menaquinone was MK-9, and 2,4-diaminobutyric acid and ornithine were the diagnostic diamino acids in the cell-wall peptidoglycan.
32809927	7	78	theme	diamino	1181:1187	arg1	acid					1142:1145	2,4-diaminobutyric acid	1123:1145	2,4-diaminobutyric acid	1123:1145	The predominant menaquinone was MK-9, and 2,4-diaminobutyric acid and ornithine were the diagnostic diamino acids in the cell-wall peptidoglycan.
32809927	4	79	theme	strain	442:447	arg1	member					469:474	a member	467:474	a member of the genus Gulosibacter	467:500	Similarly, analysis based on 16S rRNA gene sequences showed that strain YIM 102482-1T was a member of the genus Gulosibacter and most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T (96.0 %), respectively.
32809927	4	79	theme	strain	442:447	arg1	102482-1T					453:461	strain YIM 102482-1T	442:461	strain YIM 102482-1T	442:461	Similarly, analysis based on 16S rRNA gene sequences showed that strain YIM 102482-1T was a member of the genus Gulosibacter and most closely related to Gulosibacter feacalis NBRC 15706T (97.6 %), Gulosibacter bifidus NBRC 103089T (97.6 %), Gulosibacter chungangensis KCTC 13959T (96.4 %) and Gulosibacter molinativorax DSM 13485T (96.0 %), respectively.
32809927	1	80	theme	novel	33:37	arg1	actinobacterium					39:53	a novel actinobacterium	31:53	a novel actinobacterium	31:53	nov., a novel actinobacterium isolated from Macaca mulatta faeces.
32809927	1	80	theme	novel	33:37	arg1	nov.					25:28	nov.	25:28	nov.	25:28	nov., a novel actinobacterium isolated from Macaca mulatta faeces.
32809927	10	81	theme	chemotaxonomic	1452:1465	arg1	data					1467:1470	physiological, biochemical and chemotaxonomic data	1421:1470	physiological, biochemical and chemotaxonomic data	1421:1470	Based on analysis results of physiological, biochemical and chemotaxonomic data, strain YIM 102482-1T represents a novel species of the genus Gulosibacter, for which the name Gulosibacter macacae sp.
32809927	5	82	theme	rRNA	777:780	arg1	sequences					787:795	16S rRNA gene sequences	773:795	16S rRNA gene sequences	773:795	Furthermore, phylogenetic trees based on 16S rRNA gene sequences and genomic sequences demonstrated that strain YIM 102482-1T formed a distinct branch with all type strains of the genus Gulosibacter.
32809927	10	83	theme	Gulosibacter	1567:1578	arg1	sp					1588:1589	the name Gulosibacter macacae sp	1558:1589	the name Gulosibacter macacae sp	1558:1589	Based on analysis results of physiological, biochemical and chemotaxonomic data, strain YIM 102482-1T represents a novel species of the genus Gulosibacter, for which the name Gulosibacter macacae sp.
31730033	6	0	theme	cellular	524:531	arg1	iso-C16 					550:557	iso-C16 	550:557	iso-C16 	550:557	The predominant cellular fatty acids were iso-C16 : 0 and C16 : 0.
31730033	6	0	theme	cellular	524:531	arg1	acids					539:543	The predominant cellular fatty acids	508:543	The predominant cellular fatty acids	508:543	The predominant cellular fatty acids were iso-C16 : 0 and C16 : 0.
31730033	9	1	theme	polyphasic	960:969	arg1	identity					1024:1031	average nucleotide identity	1005:1031	average nucleotide identity	1005:1031	Based on the polyphasic approach, DNA-DNA relatedness and average nucleotide identity (ANI), the strain is considered to represent a novel species of the genus Microbispora, for which the name Microbispora catharanthi is proposed.
31730033	9	1	theme	polyphasic	960:969	arg1	approach					971:978	the polyphasic approach	956:978	the polyphasic approach	956:978	Based on the polyphasic approach, DNA-DNA relatedness and average nucleotide identity (ANI), the strain is considered to represent a novel species of the genus Microbispora, for which the name Microbispora catharanthi is proposed.
31730033	9	1	theme	polyphasic	960:969	arg1	relatedness					989:999	DNA-DNA relatedness	981:999	DNA-DNA relatedness	981:999	Based on the polyphasic approach, DNA-DNA relatedness and average nucleotide identity (ANI), the strain is considered to represent a novel species of the genus Microbispora, for which the name Microbispora catharanthi is proposed.
31730033	8	2	with	similarity	888:897	arg1	104650T					929:935	Microbispora tritici DSM 104650T	904:935	Microbispora tritici DSM 104650T (99.5 %)	904:944	Strain CR1-09T showed the highest 16S rRNA gene sequence similarity with Microbispora tritici DSM 104650T (99.5 %).
31730033	8	2	with	similarity	888:897	arg1	%					943:943	99.5 %	938:943	99.5 %	938:943	Strain CR1-09T showed the highest 16S rRNA gene sequence similarity with Microbispora tritici DSM 104650T (99.5 %).
31730033	7	3	theme	phosphatidylinositol	742:761	arg1	mannosides					763:772	phosphatidylinositol mannosides	742:772	phosphatidylinositol mannosides	742:772	The polar lipids profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxyl phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, and unidentified ninhydrin positive phosphoglycolipids.
31730033	6	4	theme	predominant	512:522	arg1	iso-C16 					550:557	iso-C16 	550:557	iso-C16 	550:557	The predominant cellular fatty acids were iso-C16 : 0 and C16 : 0.
31730033	6	4	theme	predominant	512:522	arg1	acids					539:543	The predominant cellular fatty acids	508:543	The predominant cellular fatty acids	508:543	The predominant cellular fatty acids were iso-C16 : 0 and C16 : 0.
31730033	8	5	theme	tritici	917:923	arg1	104650T					929:935	Microbispora tritici DSM 104650T	904:935	Microbispora tritici DSM 104650T (99.5 %)	904:944	Strain CR1-09T showed the highest 16S rRNA gene sequence similarity with Microbispora tritici DSM 104650T (99.5 %).
31730033	8	5	theme	tritici	917:923	arg1	%					943:943	99.5 %	938:943	99.5 %	938:943	Strain CR1-09T showed the highest 16S rRNA gene sequence similarity with Microbispora tritici DSM 104650T (99.5 %).
31730033	10	6	theme	30045T=TISTR	1218:1229	arg1	CR1-09T					1204:1210	strain CR1-09T	1197:1210	strain CR1-09T (=JCM 30045T=TISTR 2273T)	1197:1236	The type strain is strain CR1-09T (=JCM 30045T=TISTR 2273T).
31730033	10	6	theme	30045T=TISTR	1218:1229	arg1	2273T					1231:1235	=JCM 30045T=TISTR 2273T	1213:1235	=JCM 30045T=TISTR 2273T	1213:1235	The type strain is strain CR1-09T (=JCM 30045T=TISTR 2273T).
31730033	8	7	theme	DSM	925:927	arg1	104650T					929:935	Microbispora tritici DSM 104650T	904:935	Microbispora tritici DSM 104650T (99.5 %)	904:944	Strain CR1-09T showed the highest 16S rRNA gene sequence similarity with Microbispora tritici DSM 104650T (99.5 %).
31730033	8	7	theme	DSM	925:927	arg1	%					943:943	99.5 %	938:943	99.5 %	938:943	Strain CR1-09T showed the highest 16S rRNA gene sequence similarity with Microbispora tritici DSM 104650T (99.5 %).
31730033	4	8	from	acid	421:424	arg1	peptidoglycans					439:452	cell wall peptidoglycans	429:452	cell wall peptidoglycans	429:452	It contained meso-diaminopimelic acid in cell wall peptidoglycans.
31730033	8	9	theme	Microbispora	904:915	arg1	104650T					929:935	Microbispora tritici DSM 104650T	904:935	Microbispora tritici DSM 104650T (99.5 %)	904:944	Strain CR1-09T showed the highest 16S rRNA gene sequence similarity with Microbispora tritici DSM 104650T (99.5 %).
31730033	8	9	theme	Microbispora	904:915	arg1	%					943:943	99.5 %	938:943	99.5 %	938:943	Strain CR1-09T showed the highest 16S rRNA gene sequence similarity with Microbispora tritici DSM 104650T (99.5 %).
31730033	8	10	theme	highest	857:863	arg1	similarity					888:897	the highest 16S rRNA gene sequence similarity	853:897	the highest 16S rRNA gene sequence similarity with Microbispora tritici DSM 104650T (99.5 %)	853:944	Strain CR1-09T showed the highest 16S rRNA gene sequence similarity with Microbispora tritici DSM 104650T (99.5 %).
31730033	3	11	dep	ovular	282:287	arg1	to					289:290	to	289:290	to	289:290	The isolate formed a pair of ovular to circular, smooth-surfaced spores on short sporophores alternately branched from aerial mycelia.
31730033	3	11	dep	ovular	282:287	arg1	smooth-surfaced					302:316	smooth-surfaced	302:316	smooth-surfaced	302:316	The isolate formed a pair of ovular to circular, smooth-surfaced spores on short sporophores alternately branched from aerial mycelia.
31730033	3	11	dep	ovular	282:287	arg1	circular					292:299	circular	292:299	circular	292:299	The isolate formed a pair of ovular to circular, smooth-surfaced spores on short sporophores alternately branched from aerial mycelia.
31730033	7	12	theme	polar	579:583	arg1	profile					592:598	The polar lipids profile	575:598	The polar lipids profile	575:598	The polar lipids profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxyl phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, and unidentified ninhydrin positive phosphoglycolipids.
31730033	3	13	theme	short	328:332	arg1	sporophores					334:344	short sporophores	328:344	short sporophores alternately branched from aerial mycelia	328:385	The isolate formed a pair of ovular to circular, smooth-surfaced spores on short sporophores alternately branched from aerial mycelia.
31730033	7	14	theme	lipids	585:590	arg1	profile					592:598	The polar lipids profile	575:598	The polar lipids profile	575:598	The polar lipids profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxyl phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, and unidentified ninhydrin positive phosphoglycolipids.
31730033	4	15	theme	meso-diaminopimelic	401:419	arg1	acid					421:424	meso-diaminopimelic acid	401:424	meso-diaminopimelic acid in cell wall peptidoglycans	401:452	It contained meso-diaminopimelic acid in cell wall peptidoglycans.
31730033	8	16	theme	sequence	879:886	arg1	similarity					888:897	the highest 16S rRNA gene sequence similarity	853:897	the highest 16S rRNA gene sequence similarity with Microbispora tritici DSM 104650T (99.5 %)	853:944	Strain CR1-09T showed the highest 16S rRNA gene sequence similarity with Microbispora tritici DSM 104650T (99.5 %).
31730033	10	17	theme	strain	1197:1202	arg1	strain					1187:1192	The type strain	1178:1192	The type strain	1178:1192	The type strain is strain CR1-09T (=JCM 30045T=TISTR 2273T).
31730033	10	17	theme	strain	1197:1202	arg1	CR1-09T					1204:1210	strain CR1-09T	1197:1210	strain CR1-09T (=JCM 30045T=TISTR 2273T)	1197:1236	The type strain is strain CR1-09T (=JCM 30045T=TISTR 2273T).
31730033	10	17	theme	strain	1197:1202	arg1	2273T					1231:1235	=JCM 30045T=TISTR 2273T	1213:1235	=JCM 30045T=TISTR 2273T	1213:1235	The type strain is strain CR1-09T (=JCM 30045T=TISTR 2273T).
31730033	10	18	theme	=JCM	1213:1216	arg1	CR1-09T					1204:1210	strain CR1-09T	1197:1210	strain CR1-09T (=JCM 30045T=TISTR 2273T)	1197:1236	The type strain is strain CR1-09T (=JCM 30045T=TISTR 2273T).
31730033	10	18	theme	=JCM	1213:1216	arg1	2273T					1231:1235	=JCM 30045T=TISTR 2273T	1213:1235	=JCM 30045T=TISTR 2273T	1213:1235	The type strain is strain CR1-09T (=JCM 30045T=TISTR 2273T).
31730033	9	19	theme	nucleotide	1013:1022	arg1	identity					1024:1031	average nucleotide identity	1005:1031	average nucleotide identity	1005:1031	Based on the polyphasic approach, DNA-DNA relatedness and average nucleotide identity (ANI), the strain is considered to represent a novel species of the genus Microbispora, for which the name Microbispora catharanthi is proposed.
31730033	9	19	theme	nucleotide	1013:1022	arg1	approach					971:978	the polyphasic approach	956:978	the polyphasic approach	956:978	Based on the polyphasic approach, DNA-DNA relatedness and average nucleotide identity (ANI), the strain is considered to represent a novel species of the genus Microbispora, for which the name Microbispora catharanthi is proposed.
31730033	9	20	theme	genus	1101:1105	arg1	Microbispora					1107:1118	the genus Microbispora	1097:1118	the genus Microbispora	1097:1118	Based on the polyphasic approach, DNA-DNA relatedness and average nucleotide identity (ANI), the strain is considered to represent a novel species of the genus Microbispora, for which the name Microbispora catharanthi is proposed.
31730033	0	21	theme	catharanthi	13:23	arg1	sp					25:26	Microbispora catharanthi sp	0:26	Microbispora catharanthi sp.	0:27	Microbispora catharanthi sp.
31730033	9	22	theme	Microbispora	1107:1118	arg1	species					1086:1092	a novel species	1078:1092	a novel species	1078:1092	Based on the polyphasic approach, DNA-DNA relatedness and average nucleotide identity (ANI), the strain is considered to represent a novel species of the genus Microbispora, for which the name Microbispora catharanthi is proposed.
31730033	0	23	theme	Microbispora	0:11	arg1	sp					25:26	Microbispora catharanthi sp	0:26	Microbispora catharanthi sp.	0:27	Microbispora catharanthi sp.
31730033	6	24	dep	iso-C16 	550:557	arg1	C16 					566:569	C16 	566:569	C16 	566:569	The predominant cellular fatty acids were iso-C16 : 0 and C16 : 0.
31730033	6	24	dep	iso-C16 	550:557	arg1	 0					559:560	 0	559:560	 0	559:560	The predominant cellular fatty acids were iso-C16 : 0 and C16 : 0.
31730033	3	25	theme	aerial	372:377	arg1	mycelia					379:385	aerial mycelia	372:385	aerial mycelia	372:385	The isolate formed a pair of ovular to circular, smooth-surfaced spores on short sporophores alternately branched from aerial mycelia.
31730033	1	26	theme	Catharanthus	93:104	arg1	roseus					106:111	Catharanthus roseus	93:111	Catharanthus roseus	93:111	nov., a novel endophytic actinomycete isolated from the root of Catharanthus roseus.
31730033	1	27	attach	isolated	67:74	arg1	root					85:88	the root	81:88	the root of Catharanthus roseus	81:111	nov., a novel endophytic actinomycete isolated from the root of Catharanthus roseus.
31730033	1	27	attach	isolated	67:74	arg2	actinomycete					54:65	a novel endophytic actinomycete	35:65	a novel endophytic actinomycete isolated from the root of Catharanthus roseus	35:111	nov., a novel endophytic actinomycete isolated from the root of Catharanthus roseus.
31730033	6	28	dep	 0	559:560	arg1	 0					571:572	 0	571:572	 0	571:572	The predominant cellular fatty acids were iso-C16 : 0 and C16 : 0.
31730033	9	29	theme	novel	1080:1084	arg1	species					1086:1092	a novel species	1078:1092	a novel species	1078:1092	Based on the polyphasic approach, DNA-DNA relatedness and average nucleotide identity (ANI), the strain is considered to represent a novel species of the genus Microbispora, for which the name Microbispora catharanthi is proposed.
31730033	10	30	theme	type	1182:1185	arg1	strain					1187:1192	The type strain	1178:1192	The type strain	1178:1192	The type strain is strain CR1-09T (=JCM 30045T=TISTR 2273T).
31730033	10	30	theme	type	1182:1185	arg1	CR1-09T					1204:1210	strain CR1-09T	1197:1210	strain CR1-09T (=JCM 30045T=TISTR 2273T)	1197:1236	The type strain is strain CR1-09T (=JCM 30045T=TISTR 2273T).
31730033	9	31	theme	name	1135:1138	arg1	catharanthi					1153:1163	the name Microbispora catharanthi	1131:1163	the name Microbispora catharanthi	1131:1163	Based on the polyphasic approach, DNA-DNA relatedness and average nucleotide identity (ANI), the strain is considered to represent a novel species of the genus Microbispora, for which the name Microbispora catharanthi is proposed.
31730033	7	32	theme	hydroxyl	685:692	arg1	phosphatidylethanolamine					694:717	hydroxyl phosphatidylethanolamine	685:717	hydroxyl phosphatidylethanolamine	685:717	The polar lipids profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxyl phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, and unidentified ninhydrin positive phosphoglycolipids.
31730033	9	33	theme	Microbispora	1140:1151	arg1	catharanthi					1153:1163	the name Microbispora catharanthi	1131:1163	the name Microbispora catharanthi	1131:1163	Based on the polyphasic approach, DNA-DNA relatedness and average nucleotide identity (ANI), the strain is considered to represent a novel species of the genus Microbispora, for which the name Microbispora catharanthi is proposed.
31730033	1	34	theme	roseus	106:111	arg1	root					85:88	the root	81:88	the root of Catharanthus roseus	81:111	nov., a novel endophytic actinomycete isolated from the root of Catharanthus roseus.
31730033	4	35	contain	contained	391:399	arg2	acid					421:424	meso-diaminopimelic acid	401:424	meso-diaminopimelic acid in cell wall peptidoglycans	401:452	It contained meso-diaminopimelic acid in cell wall peptidoglycans.
31730033	4	35	contain	contained	391:399	arg1	It					388:389	It	388:389	It	388:389	It contained meso-diaminopimelic acid in cell wall peptidoglycans.
31730033	7	36	theme	positive	802:809	arg1	phosphoglycolipids					811:828	unidentified ninhydrin positive phosphoglycolipids	779:828	unidentified ninhydrin positive phosphoglycolipids	779:828	The polar lipids profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxyl phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, and unidentified ninhydrin positive phosphoglycolipids.
31730033	4	37	theme	cell	429:432	arg1	peptidoglycans					439:452	cell wall peptidoglycans	429:452	cell wall peptidoglycans	429:452	It contained meso-diaminopimelic acid in cell wall peptidoglycans.
31730033	2	38	attach	isolated	146:153	arg1	root					164:167	the root	160:167	the root of Catharanthus roseus	160:190	Strain CR1-09T, an actinomycete isolated from the root of Catharanthus roseus, was taxonomically studied based upon polyphasic approaches.
31730033	2	38	attach	isolated	146:153	arg2	actinomycete					133:144	an actinomycete	130:144	an actinomycete isolated from the root of Catharanthus roseus	130:190	Strain CR1-09T, an actinomycete isolated from the root of Catharanthus roseus, was taxonomically studied based upon polyphasic approaches.
31730033	2	38	attach	isolated	146:153	arg2	Strain					114:119	Strain CR1-09T	114:127	Strain CR1-09T	114:127	Strain CR1-09T, an actinomycete isolated from the root of Catharanthus roseus, was taxonomically studied based upon polyphasic approaches.
31730033	8	39	theme	rRNA	869:872	arg1	similarity					888:897	the highest 16S rRNA gene sequence similarity	853:897	the highest 16S rRNA gene sequence similarity with Microbispora tritici DSM 104650T (99.5 %)	853:944	Strain CR1-09T showed the highest 16S rRNA gene sequence similarity with Microbispora tritici DSM 104650T (99.5 %).
31730033	1	40	theme	novel	37:41	arg1	actinomycete					54:65	a novel endophytic actinomycete	35:65	a novel endophytic actinomycete isolated from the root of Catharanthus roseus	35:111	nov., a novel endophytic actinomycete isolated from the root of Catharanthus roseus.
31730033	8	41	theme	16S	865:867	arg1	similarity					888:897	the highest 16S rRNA gene sequence similarity	853:897	the highest 16S rRNA gene sequence similarity with Microbispora tritici DSM 104650T (99.5 %)	853:944	Strain CR1-09T showed the highest 16S rRNA gene sequence similarity with Microbispora tritici DSM 104650T (99.5 %).
31730033	7	42	theme	unidentified	779:790	arg1	phosphoglycolipids					811:828	unidentified ninhydrin positive phosphoglycolipids	779:828	unidentified ninhydrin positive phosphoglycolipids	779:828	The polar lipids profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxyl phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, and unidentified ninhydrin positive phosphoglycolipids.
31730033	3	43	theme	ovular	282:287	arg1	spores					318:323	ovular to circular, smooth-surfaced spores	282:323	ovular to circular, smooth-surfaced spores	282:323	The isolate formed a pair of ovular to circular, smooth-surfaced spores on short sporophores alternately branched from aerial mycelia.
31730033	2	44	theme	roseus	185:190	arg1	root					164:167	the root	160:167	the root of Catharanthus roseus	160:190	Strain CR1-09T, an actinomycete isolated from the root of Catharanthus roseus, was taxonomically studied based upon polyphasic approaches.
31730033	1	45	theme	endophytic	43:52	arg1	actinomycete					54:65	a novel endophytic actinomycete	35:65	a novel endophytic actinomycete isolated from the root of Catharanthus roseus	35:111	nov., a novel endophytic actinomycete isolated from the root of Catharanthus roseus.
31730033	1	46	dep	actinomycete	54:65	arg1	nov.					29:32	nov.	29:32	nov.	29:32	nov., a novel endophytic actinomycete isolated from the root of Catharanthus roseus.
31730033	5	47	theme	major	459:463	arg1	MK-9					483:486	MK-9	483:486	MK-9	483:486	The major menaquinones were MK-9 (H4) and MK-9 (H2).
31730033	5	47	theme	major	459:463	arg1	menaquinones					465:476	The major menaquinones	455:476	The major menaquinones	455:476	The major menaquinones were MK-9 (H4) and MK-9 (H2).
31730033	7	48	theme	ninhydrin	792:800	arg1	phosphoglycolipids					811:828	unidentified ninhydrin positive phosphoglycolipids	779:828	unidentified ninhydrin positive phosphoglycolipids	779:828	The polar lipids profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxyl phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, and unidentified ninhydrin positive phosphoglycolipids.
31730033	3	49	theme	spores	318:323	arg1	pair					274:277	a pair	272:277	a pair of ovular to circular, smooth-surfaced spores	272:323	The isolate formed a pair of ovular to circular, smooth-surfaced spores on short sporophores alternately branched from aerial mycelia.
31730033	4	50	theme	wall	434:437	arg1	peptidoglycans					439:452	cell wall peptidoglycans	429:452	cell wall peptidoglycans	429:452	It contained meso-diaminopimelic acid in cell wall peptidoglycans.
31730033	8	51	theme	gene	874:877	arg1	similarity					888:897	the highest 16S rRNA gene sequence similarity	853:897	the highest 16S rRNA gene sequence similarity with Microbispora tritici DSM 104650T (99.5 %)	853:944	Strain CR1-09T showed the highest 16S rRNA gene sequence similarity with Microbispora tritici DSM 104650T (99.5 %).
31730033	8	52	theme	Strain	831:836	arg1	CR1-09T					838:844	Strain CR1-09T	831:844	Strain CR1-09T	831:844	Strain CR1-09T showed the highest 16S rRNA gene sequence similarity with Microbispora tritici DSM 104650T (99.5 %).
31730033	6	53	theme	fatty	533:537	arg1	iso-C16 					550:557	iso-C16 	550:557	iso-C16 	550:557	The predominant cellular fatty acids were iso-C16 : 0 and C16 : 0.
31730033	6	53	theme	fatty	533:537	arg1	acids					539:543	The predominant cellular fatty acids	508:543	The predominant cellular fatty acids	508:543	The predominant cellular fatty acids were iso-C16 : 0 and C16 : 0.
31730033	2	54	theme	polyphasic	230:239	arg1	approaches					241:250	polyphasic approaches	230:250	polyphasic approaches	230:250	Strain CR1-09T, an actinomycete isolated from the root of Catharanthus roseus, was taxonomically studied based upon polyphasic approaches.
31730033	9	55	theme	average	1005:1011	arg1	identity					1024:1031	average nucleotide identity	1005:1031	average nucleotide identity	1005:1031	Based on the polyphasic approach, DNA-DNA relatedness and average nucleotide identity (ANI), the strain is considered to represent a novel species of the genus Microbispora, for which the name Microbispora catharanthi is proposed.
31730033	9	55	theme	average	1005:1011	arg1	approach					971:978	the polyphasic approach	956:978	the polyphasic approach	956:978	Based on the polyphasic approach, DNA-DNA relatedness and average nucleotide identity (ANI), the strain is considered to represent a novel species of the genus Microbispora, for which the name Microbispora catharanthi is proposed.
31730033	9	56	theme	DNA-DNA	981:987	arg1	approach					971:978	the polyphasic approach	956:978	the polyphasic approach	956:978	Based on the polyphasic approach, DNA-DNA relatedness and average nucleotide identity (ANI), the strain is considered to represent a novel species of the genus Microbispora, for which the name Microbispora catharanthi is proposed.
31730033	9	56	theme	DNA-DNA	981:987	arg1	relatedness					989:999	DNA-DNA relatedness	981:999	DNA-DNA relatedness	981:999	Based on the polyphasic approach, DNA-DNA relatedness and average nucleotide identity (ANI), the strain is considered to represent a novel species of the genus Microbispora, for which the name Microbispora catharanthi is proposed.
31730033	9	56	theme	DNA-DNA	981:987	arg1	ANI					1034:1036	ANI	1034:1036	ANI	1034:1036	Based on the polyphasic approach, DNA-DNA relatedness and average nucleotide identity (ANI), the strain is considered to represent a novel species of the genus Microbispora, for which the name Microbispora catharanthi is proposed.
34366479	0	0	theme	polysaccharides	82:96	arg1	characteristics					36:50	structural characteristics	25:50	structural characteristics	25:50	Effects of drying on the structural characteristics and antioxidant activities of polysaccharides from Stropharia rugosoannulata.
34366479	0	0	theme	polysaccharides	82:96	arg1	activities					68:77	antioxidant activities	56:77	antioxidant activities	56:77	Effects of drying on the structural characteristics and antioxidant activities of polysaccharides from Stropharia rugosoannulata.
34366479	4	1	dep	characteristics	699:713	arg1	the					684:686	the	684:686	the	684:686	We found that drying can affect the structural characteristics and antioxidant activities of SRPs.
34366479	5	2	theme	monosaccharide	758:771	arg1	compositions					773:784	Varied monosaccharide compositions	751:784	Varied monosaccharide compositions	751:784	Varied monosaccharide compositions were found in FSRP-1, FSRP-2, HSRP-1 and HSRP-2, and HAD-treated SRP had more glucose and less galactose.
34366479	3	3	from	rugosoannulata	478:491	arg1	Polysaccharides					444:458	Polysaccharides	444:458	Polysaccharides from the dried S. rugosoannulata (SRP)	444:497	Polysaccharides from the dried S. rugosoannulata (SRP) were purified using a DEAE-52 cellulose column to obtain two types of neutral SRPs (FSRP-1 and HSRP-1) and two types of acidic SRPs (FSRP-2 and HSRP-2).
34366479	1	4	theme	antioxidant	181:191	arg1	activities					193:202	antioxidant activities	181:202	antioxidant activities	181:202	We investigated the structural characteristics and antioxidant activities of two types of neutral polysaccharides and two types of acidic polysaccharides from Stropharia rugosoannulata under different drying methods.
34366479	9	5	theme	Hot-air	1353:1359	arg1	candidate					1430:1438	a suitable candidate	1419:1438	a suitable candidate for use in the preparation of antioxidant polysaccharides as functional foods	1419:1516	Hot-air dried S. rugosoannulata could therefore be recommended as a suitable candidate for use in the preparation of antioxidant polysaccharides as functional foods.
34366479	9	5	theme	Hot-air	1353:1359	arg1	rugosoannulata					1370:1383	Hot-air dried S. rugosoannulata	1353:1383	Hot-air dried S. rugosoannulata	1353:1383	Hot-air dried S. rugosoannulata could therefore be recommended as a suitable candidate for use in the preparation of antioxidant polysaccharides as functional foods.
34366479	6	6	from	structure	1011:1019	arg1	HSRP-2					1035:1040	HSRP-2	1035:1040	HSRP-2	1035:1040	The (1 → 6)-α-D-Galp linkage was the primary chain in FSRP-1 and HSRP-1, whereas the (1 → 3)-β-D-Glcp was the backbone structure in FSRP-2 and HSRP-2.
34366479	6	6	from	structure	1011:1019	arg1	FSRP-2					1024:1029	FSRP-2	1024:1029	FSRP-2	1024:1029	The (1 → 6)-α-D-Galp linkage was the primary chain in FSRP-1 and HSRP-1, whereas the (1 → 3)-β-D-Glcp was the backbone structure in FSRP-2 and HSRP-2.
34366479	9	7	theme	polysaccharides	1482:1496	arg1	preparation					1455:1465	the preparation	1451:1465	the preparation of antioxidant polysaccharides as functional foods	1451:1516	Hot-air dried S. rugosoannulata could therefore be recommended as a suitable candidate for use in the preparation of antioxidant polysaccharides as functional foods.
34366479	1	8	theme	Stropharia	289:298	arg1	rugosoannulata					300:313	Stropharia rugosoannulata	289:313	Stropharia rugosoannulata	289:313	We investigated the structural characteristics and antioxidant activities of two types of neutral polysaccharides and two types of acidic polysaccharides from Stropharia rugosoannulata under different drying methods.
34366479	0	9	theme	Stropharia	103:112	arg1	rugosoannulata					114:127	Stropharia rugosoannulata	103:127	Stropharia rugosoannulata	103:127	Effects of drying on the structural characteristics and antioxidant activities of polysaccharides from Stropharia rugosoannulata.
34366479	0	10	from	Effects	0:6	arg1	characteristics					36:50	structural characteristics	25:50	structural characteristics	25:50	Effects of drying on the structural characteristics and antioxidant activities of polysaccharides from Stropharia rugosoannulata.
34366479	0	10	from	Effects	0:6	arg1	activities					68:77	antioxidant activities	56:77	antioxidant activities	56:77	Effects of drying on the structural characteristics and antioxidant activities of polysaccharides from Stropharia rugosoannulata.
34366479	4	11	theme	antioxidant	719:729	arg1	activities					731:740	antioxidant activities	719:740	antioxidant activities	719:740	We found that drying can affect the structural characteristics and antioxidant activities of SRPs.
34366479	9	12	theme	S.	1367:1368	arg1	candidate					1430:1438	a suitable candidate	1419:1438	a suitable candidate for use in the preparation of antioxidant polysaccharides as functional foods	1419:1516	Hot-air dried S. rugosoannulata could therefore be recommended as a suitable candidate for use in the preparation of antioxidant polysaccharides as functional foods.
34366479	9	12	theme	S.	1367:1368	arg1	rugosoannulata					1370:1383	Hot-air dried S. rugosoannulata	1353:1383	Hot-air dried S. rugosoannulata	1353:1383	Hot-air dried S. rugosoannulata could therefore be recommended as a suitable candidate for use in the preparation of antioxidant polysaccharides as functional foods.
34366479	8	13	theme	antioxidant	1313:1323	arg1	activity					1325:1332	a stronger antioxidant activity	1302:1332	a stronger antioxidant activity	1302:1332	FSRP-1, FSRP-2, HSRP-1 and HSRP-2 had positive ferric ion reducing antioxidant power and scavenging activities on ABTS+ and hydroxyl radicals, whereas HSRP exhibited a stronger antioxidant activity than that of FSRP.
34366479	3	14	theme	neutral	569:575	arg1	HSRP-1					594:599	HSRP-1	594:599	HSRP-1	594:599	Polysaccharides from the dried S. rugosoannulata (SRP) were purified using a DEAE-52 cellulose column to obtain two types of neutral SRPs (FSRP-1 and HSRP-1) and two types of acidic SRPs (FSRP-2 and HSRP-2).
34366479	3	14	theme	neutral	569:575	arg1	FSRP-1					583:588	FSRP-1	583:588	FSRP-1	583:588	Polysaccharides from the dried S. rugosoannulata (SRP) were purified using a DEAE-52 cellulose column to obtain two types of neutral SRPs (FSRP-1 and HSRP-1) and two types of acidic SRPs (FSRP-2 and HSRP-2).
34366479	3	14	theme	neutral	569:575	arg1	SRPs					577:580	neutral SRPs	569:580	neutral SRPs (FSRP-1 and HSRP-1)	569:600	Polysaccharides from the dried S. rugosoannulata (SRP) were purified using a DEAE-52 cellulose column to obtain two types of neutral SRPs (FSRP-1 and HSRP-1) and two types of acidic SRPs (FSRP-2 and HSRP-2).
34366479	3	15	theme	cellulose	529:537	arg1	column					539:544	a DEAE-52 cellulose column	519:544	a DEAE-52 cellulose column	519:544	Polysaccharides from the dried S. rugosoannulata (SRP) were purified using a DEAE-52 cellulose column to obtain two types of neutral SRPs (FSRP-1 and HSRP-1) and two types of acidic SRPs (FSRP-2 and HSRP-2).
34366479	0	16	from	characteristics	36:50	arg1	rugosoannulata					114:127	Stropharia rugosoannulata	103:127	Stropharia rugosoannulata	103:127	Effects of drying on the structural characteristics and antioxidant activities of polysaccharides from Stropharia rugosoannulata.
34366479	8	17	theme	reducing	1194:1201	arg1	power					1215:1219	positive ferric ion reducing antioxidant power	1174:1219	positive ferric ion reducing antioxidant power	1174:1219	FSRP-1, FSRP-2, HSRP-1 and HSRP-2 had positive ferric ion reducing antioxidant power and scavenging activities on ABTS+ and hydroxyl radicals, whereas HSRP exhibited a stronger antioxidant activity than that of FSRP.
34366479	8	18	theme	ion	1190:1192	arg1	power					1215:1219	positive ferric ion reducing antioxidant power	1174:1219	positive ferric ion reducing antioxidant power	1174:1219	FSRP-1, FSRP-2, HSRP-1 and HSRP-2 had positive ferric ion reducing antioxidant power and scavenging activities on ABTS+ and hydroxyl radicals, whereas HSRP exhibited a stronger antioxidant activity than that of FSRP.
34366479	5	19	theme	HAD-treated	839:849	arg1	SRP					851:853	HAD-treated SRP	839:853	HAD-treated SRP	839:853	Varied monosaccharide compositions were found in FSRP-1, FSRP-2, HSRP-1 and HSRP-2, and HAD-treated SRP had more glucose and less galactose.
34366479	5	20	located	found	791:795	arg2	compositions					773:784	Varied monosaccharide compositions	751:784	Varied monosaccharide compositions	751:784	Varied monosaccharide compositions were found in FSRP-1, FSRP-2, HSRP-1 and HSRP-2, and HAD-treated SRP had more glucose and less galactose.
34366479	5	20	located	found	791:795	arg1	FSRP-2					808:813	FSRP-2	808:813	FSRP-2	808:813	Varied monosaccharide compositions were found in FSRP-1, FSRP-2, HSRP-1 and HSRP-2, and HAD-treated SRP had more glucose and less galactose.
34366479	5	20	located	found	791:795	arg1	FSRP-1					800:805	FSRP-1	800:805	FSRP-1	800:805	Varied monosaccharide compositions were found in FSRP-1, FSRP-2, HSRP-1 and HSRP-2, and HAD-treated SRP had more glucose and less galactose.
34366479	5	20	located	found	791:795	arg1	HSRP-2					827:832	HSRP-2	827:832	HSRP-2	827:832	Varied monosaccharide compositions were found in FSRP-1, FSRP-2, HSRP-1 and HSRP-2, and HAD-treated SRP had more glucose and less galactose.
34366479	5	20	located	found	791:795	arg1	HSRP-1					816:821	HSRP-1	816:821	HSRP-1	816:821	Varied monosaccharide compositions were found in FSRP-1, FSRP-2, HSRP-1 and HSRP-2, and HAD-treated SRP had more glucose and less galactose.
34366479	9	21	theme	antioxidant	1470:1480	arg1	polysaccharides					1482:1496	antioxidant polysaccharides	1470:1496	antioxidant polysaccharides as functional foods	1470:1516	Hot-air dried S. rugosoannulata could therefore be recommended as a suitable candidate for use in the preparation of antioxidant polysaccharides as functional foods.
34366479	8	22	contain	had	1170:1172	arg1	FSRP-2					1144:1149	FSRP-2	1144:1149	FSRP-2	1144:1149	FSRP-1, FSRP-2, HSRP-1 and HSRP-2 had positive ferric ion reducing antioxidant power and scavenging activities on ABTS+ and hydroxyl radicals, whereas HSRP exhibited a stronger antioxidant activity than that of FSRP.
34366479	8	22	contain	had	1170:1172	arg2	power					1215:1219	positive ferric ion reducing antioxidant power	1174:1219	positive ferric ion reducing antioxidant power	1174:1219	FSRP-1, FSRP-2, HSRP-1 and HSRP-2 had positive ferric ion reducing antioxidant power and scavenging activities on ABTS+ and hydroxyl radicals, whereas HSRP exhibited a stronger antioxidant activity than that of FSRP.
34366479	8	22	contain	had	1170:1172	arg1	HSRP-1					1152:1157	HSRP-1	1152:1157	HSRP-1	1152:1157	FSRP-1, FSRP-2, HSRP-1 and HSRP-2 had positive ferric ion reducing antioxidant power and scavenging activities on ABTS+ and hydroxyl radicals, whereas HSRP exhibited a stronger antioxidant activity than that of FSRP.
34366479	8	22	contain	had	1170:1172	arg1	HSRP-2					1163:1168	HSRP-2	1163:1168	HSRP-2	1163:1168	FSRP-1, FSRP-2, HSRP-1 and HSRP-2 had positive ferric ion reducing antioxidant power and scavenging activities on ABTS+ and hydroxyl radicals, whereas HSRP exhibited a stronger antioxidant activity than that of FSRP.
34366479	8	22	contain	had	1170:1172	arg1	FSRP-1					1136:1141	FSRP-1	1136:1141	FSRP-1	1136:1141	FSRP-1, FSRP-2, HSRP-1 and HSRP-2 had positive ferric ion reducing antioxidant power and scavenging activities on ABTS+ and hydroxyl radicals, whereas HSRP exhibited a stronger antioxidant activity than that of FSRP.
34366479	8	22	contain	had	1170:1172	arg2	activities					1236:1245	scavenging activities	1225:1245	scavenging activities	1225:1245	FSRP-1, FSRP-2, HSRP-1 and HSRP-2 had positive ferric ion reducing antioxidant power and scavenging activities on ABTS+ and hydroxyl radicals, whereas HSRP exhibited a stronger antioxidant activity than that of FSRP.
34366479	4	23	theme	SRPs	745:748	arg1	characteristics					699:713	structural characteristics	688:713	structural characteristics	688:713	We found that drying can affect the structural characteristics and antioxidant activities of SRPs.
34366479	4	23	theme	SRPs	745:748	arg1	activities					731:740	antioxidant activities	719:740	antioxidant activities	719:740	We found that drying can affect the structural characteristics and antioxidant activities of SRPs.
34366479	7	24	from	β-configuration	1100:1114	arg1	polysaccharides					1119:1133	polysaccharides	1119:1133	polysaccharides	1119:1133	Our results thus suggest that hot air drying changed the β-configuration in polysaccharides.
34366479	1	25	theme	types	211:215	arg1	characteristics					161:175	structural characteristics	150:175	structural characteristics	150:175	We investigated the structural characteristics and antioxidant activities of two types of neutral polysaccharides and two types of acidic polysaccharides from Stropharia rugosoannulata under different drying methods.
34366479	1	25	theme	types	211:215	arg1	activities					193:202	antioxidant activities	181:202	antioxidant activities	181:202	We investigated the structural characteristics and antioxidant activities of two types of neutral polysaccharides and two types of acidic polysaccharides from Stropharia rugosoannulata under different drying methods.
34366479	1	26	theme	different	321:329	arg1	methods					338:344	different drying methods	321:344	different drying methods	321:344	We investigated the structural characteristics and antioxidant activities of two types of neutral polysaccharides and two types of acidic polysaccharides from Stropharia rugosoannulata under different drying methods.
34366479	3	27	theme	acidic	619:624	arg1	HSRP-2					643:648	HSRP-2	643:648	HSRP-2	643:648	Polysaccharides from the dried S. rugosoannulata (SRP) were purified using a DEAE-52 cellulose column to obtain two types of neutral SRPs (FSRP-1 and HSRP-1) and two types of acidic SRPs (FSRP-2 and HSRP-2).
34366479	3	27	theme	acidic	619:624	arg1	FSRP-2					632:637	FSRP-2	632:637	FSRP-2	632:637	Polysaccharides from the dried S. rugosoannulata (SRP) were purified using a DEAE-52 cellulose column to obtain two types of neutral SRPs (FSRP-1 and HSRP-1) and two types of acidic SRPs (FSRP-2 and HSRP-2).
34366479	3	27	theme	acidic	619:624	arg1	SRPs					626:629	acidic SRPs	619:629	acidic SRPs (FSRP-2 and HSRP-2)	619:649	Polysaccharides from the dried S. rugosoannulata (SRP) were purified using a DEAE-52 cellulose column to obtain two types of neutral SRPs (FSRP-1 and HSRP-1) and two types of acidic SRPs (FSRP-2 and HSRP-2).
34366479	2	28	theme	hot-air	422:428	arg1	drying					430:435	hot-air drying	422:435	hot-air drying (HAD)	422:441	Fresh S. rugosoannulata were processed with freeze-vacuum drying (FVD) and hot-air drying (HAD).
34366479	1	29	theme	drying	331:336	arg1	methods					338:344	different drying methods	321:344	different drying methods	321:344	We investigated the structural characteristics and antioxidant activities of two types of neutral polysaccharides and two types of acidic polysaccharides from Stropharia rugosoannulata under different drying methods.
34366479	1	30	from	rugosoannulata	300:313	arg1	types					211:215	two types	207:215	two types of neutral polysaccharides	207:242	We investigated the structural characteristics and antioxidant activities of two types of neutral polysaccharides and two types of acidic polysaccharides from Stropharia rugosoannulata under different drying methods.
34366479	1	30	from	rugosoannulata	300:313	arg1	types					252:256	two types	248:256	two types of acidic polysaccharides from Stropharia rugosoannulata	248:313	We investigated the structural characteristics and antioxidant activities of two types of neutral polysaccharides and two types of acidic polysaccharides from Stropharia rugosoannulata under different drying methods.
34366479	1	30	from	rugosoannulata	300:313	arg1	polysaccharides					268:282	acidic polysaccharides	261:282	acidic polysaccharides from Stropharia rugosoannulata	261:313	We investigated the structural characteristics and antioxidant activities of two types of neutral polysaccharides and two types of acidic polysaccharides from Stropharia rugosoannulata under different drying methods.
34366479	9	31	from	use	1444:1446	arg1	preparation					1455:1465	the preparation	1451:1465	the preparation of antioxidant polysaccharides as functional foods	1451:1516	Hot-air dried S. rugosoannulata could therefore be recommended as a suitable candidate for use in the preparation of antioxidant polysaccharides as functional foods.
34366479	1	32	dep	characteristics	161:175	arg1	the					146:148	the	146:148	the	146:148	We investigated the structural characteristics and antioxidant activities of two types of neutral polysaccharides and two types of acidic polysaccharides from Stropharia rugosoannulata under different drying methods.
34366479	1	33	theme	neutral	220:226	arg1	polysaccharides					228:242	neutral polysaccharides	220:242	neutral polysaccharides	220:242	We investigated the structural characteristics and antioxidant activities of two types of neutral polysaccharides and two types of acidic polysaccharides from Stropharia rugosoannulata under different drying methods.
34366479	0	34	theme	drying	11:16	arg1	Effects					0:6	Effects	0:6	Effects of drying on the structural characteristics and antioxidant activities of polysaccharides from Stropharia rugosoannulata.	0:128	Effects of drying on the structural characteristics and antioxidant activities of polysaccharides from Stropharia rugosoannulata.
34366479	6	35	theme	primary	929:935	arg1	linkage					913:919	The (1 → 6)-α-D-Galp linkage	892:919	The (1 → 6)-α-D-Galp linkage	892:919	The (1 → 6)-α-D-Galp linkage was the primary chain in FSRP-1 and HSRP-1, whereas the (1 → 3)-β-D-Glcp was the backbone structure in FSRP-2 and HSRP-2.
34366479	6	35	theme	primary	929:935	arg1	chain					937:941	the primary chain	925:941	the primary chain	925:941	The (1 → 6)-α-D-Galp linkage was the primary chain in FSRP-1 and HSRP-1, whereas the (1 → 3)-β-D-Glcp was the backbone structure in FSRP-2 and HSRP-2.
34366479	1	36	theme	polysaccharides	228:242	arg1	types					211:215	two types	207:215	two types of neutral polysaccharides	207:242	We investigated the structural characteristics and antioxidant activities of two types of neutral polysaccharides and two types of acidic polysaccharides from Stropharia rugosoannulata under different drying methods.
34366479	1	36	theme	polysaccharides	228:242	arg1	types					252:256	two types	248:256	two types of acidic polysaccharides from Stropharia rugosoannulata	248:313	We investigated the structural characteristics and antioxidant activities of two types of neutral polysaccharides and two types of acidic polysaccharides from Stropharia rugosoannulata under different drying methods.
34366479	0	37	theme	structural	25:34	arg1	characteristics					36:50	structural characteristics	25:50	structural characteristics	25:50	Effects of drying on the structural characteristics and antioxidant activities of polysaccharides from Stropharia rugosoannulata.
34366479	3	38	theme	DEAE-52	521:527	arg1	column					539:544	a DEAE-52 cellulose column	519:544	a DEAE-52 cellulose column	519:544	Polysaccharides from the dried S. rugosoannulata (SRP) were purified using a DEAE-52 cellulose column to obtain two types of neutral SRPs (FSRP-1 and HSRP-1) and two types of acidic SRPs (FSRP-2 and HSRP-2).
34366479	8	39	theme	hydroxyl	1260:1267	arg1	radicals					1269:1276	hydroxyl radicals	1260:1276	hydroxyl radicals	1260:1276	FSRP-1, FSRP-2, HSRP-1 and HSRP-2 had positive ferric ion reducing antioxidant power and scavenging activities on ABTS+ and hydroxyl radicals, whereas HSRP exhibited a stronger antioxidant activity than that of FSRP.
34366479	6	40	theme	backbone	1002:1009	arg1	structure					1011:1019	the backbone structure	998:1019	the backbone structure in FSRP-2 and HSRP-2	998:1040	The (1 → 6)-α-D-Galp linkage was the primary chain in FSRP-1 and HSRP-1, whereas the (1 → 3)-β-D-Glcp was the backbone structure in FSRP-2 and HSRP-2.
34366479	6	40	theme	backbone	1002:1009	arg1	-β-D-Glcp					984:992	the (1 → 3)-β-D-Glcp	973:992	the (1 → 3)-β-D-Glcp	973:992	The (1 → 6)-α-D-Galp linkage was the primary chain in FSRP-1 and HSRP-1, whereas the (1 → 3)-β-D-Glcp was the backbone structure in FSRP-2 and HSRP-2.
34366479	9	41	theme	suitable	1421:1428	arg1	rugosoannulata					1370:1383	Hot-air dried S. rugosoannulata	1353:1383	Hot-air dried S. rugosoannulata	1353:1383	Hot-air dried S. rugosoannulata could therefore be recommended as a suitable candidate for use in the preparation of antioxidant polysaccharides as functional foods.
34366479	9	41	theme	suitable	1421:1428	arg1	candidate					1430:1438	a suitable candidate	1419:1438	a suitable candidate for use in the preparation of antioxidant polysaccharides as functional foods	1419:1516	Hot-air dried S. rugosoannulata could therefore be recommended as a suitable candidate for use in the preparation of antioxidant polysaccharides as functional foods.
34366479	8	42	theme	positive	1174:1181	arg1	power					1215:1219	positive ferric ion reducing antioxidant power	1174:1219	positive ferric ion reducing antioxidant power	1174:1219	FSRP-1, FSRP-2, HSRP-1 and HSRP-2 had positive ferric ion reducing antioxidant power and scavenging activities on ABTS+ and hydroxyl radicals, whereas HSRP exhibited a stronger antioxidant activity than that of FSRP.
34366479	6	43	from	chain	937:941	arg1	HSRP-1					957:962	HSRP-1	957:962	HSRP-1	957:962	The (1 → 6)-α-D-Galp linkage was the primary chain in FSRP-1 and HSRP-1, whereas the (1 → 3)-β-D-Glcp was the backbone structure in FSRP-2 and HSRP-2.
34366479	6	43	from	chain	937:941	arg1	FSRP-1					946:951	FSRP-1	946:951	FSRP-1	946:951	The (1 → 6)-α-D-Galp linkage was the primary chain in FSRP-1 and HSRP-1, whereas the (1 → 3)-β-D-Glcp was the backbone structure in FSRP-2 and HSRP-2.
34366479	0	44	from	activities	68:77	arg1	rugosoannulata					114:127	Stropharia rugosoannulata	103:127	Stropharia rugosoannulata	103:127	Effects of drying on the structural characteristics and antioxidant activities of polysaccharides from Stropharia rugosoannulata.
34366479	0	45	dep	characteristics	36:50	arg1	the					21:23	the	21:23	the	21:23	Effects of drying on the structural characteristics and antioxidant activities of polysaccharides from Stropharia rugosoannulata.
34366479	3	46	dep	SRPs	626:629	arg1	HSRP-2					643:648	HSRP-2	643:648	HSRP-2	643:648	Polysaccharides from the dried S. rugosoannulata (SRP) were purified using a DEAE-52 cellulose column to obtain two types of neutral SRPs (FSRP-1 and HSRP-1) and two types of acidic SRPs (FSRP-2 and HSRP-2).
34366479	3	46	dep	SRPs	626:629	arg1	FSRP-2					632:637	FSRP-2	632:637	FSRP-2	632:637	Polysaccharides from the dried S. rugosoannulata (SRP) were purified using a DEAE-52 cellulose column to obtain two types of neutral SRPs (FSRP-1 and HSRP-1) and two types of acidic SRPs (FSRP-2 and HSRP-2).
34366479	3	46	dep	SRPs	626:629	arg1	SRPs					626:629	acidic SRPs	619:629	acidic SRPs (FSRP-2 and HSRP-2)	619:649	Polysaccharides from the dried S. rugosoannulata (SRP) were purified using a DEAE-52 cellulose column to obtain two types of neutral SRPs (FSRP-1 and HSRP-1) and two types of acidic SRPs (FSRP-2 and HSRP-2).
34366479	7	47	theme	hot	1073:1075	arg1	drying					1081:1086	hot air drying	1073:1086	hot air drying	1073:1086	Our results thus suggest that hot air drying changed the β-configuration in polysaccharides.
34366479	5	48	theme	Varied	751:756	arg1	compositions					773:784	Varied monosaccharide compositions	751:784	Varied monosaccharide compositions	751:784	Varied monosaccharide compositions were found in FSRP-1, FSRP-2, HSRP-1 and HSRP-2, and HAD-treated SRP had more glucose and less galactose.
34366479	8	49	theme	ferric	1183:1188	arg1	power					1215:1219	positive ferric ion reducing antioxidant power	1174:1219	positive ferric ion reducing antioxidant power	1174:1219	FSRP-1, FSRP-2, HSRP-1 and HSRP-2 had positive ferric ion reducing antioxidant power and scavenging activities on ABTS+ and hydroxyl radicals, whereas HSRP exhibited a stronger antioxidant activity than that of FSRP.
34366479	8	50	theme	stronger	1304:1311	arg1	activity					1325:1332	a stronger antioxidant activity	1302:1332	a stronger antioxidant activity	1302:1332	FSRP-1, FSRP-2, HSRP-1 and HSRP-2 had positive ferric ion reducing antioxidant power and scavenging activities on ABTS+ and hydroxyl radicals, whereas HSRP exhibited a stronger antioxidant activity than that of FSRP.
34366479	4	51	theme	structural	688:697	arg1	characteristics					699:713	structural characteristics	688:713	structural characteristics	688:713	We found that drying can affect the structural characteristics and antioxidant activities of SRPs.
34366479	7	52	theme	air	1077:1079	arg1	drying					1081:1086	hot air drying	1073:1086	hot air drying	1073:1086	Our results thus suggest that hot air drying changed the β-configuration in polysaccharides.
34366479	6	53	theme	-α-D-Galp	903:911	arg1	linkage					913:919	The (1 → 6)-α-D-Galp linkage	892:919	The (1 → 6)-α-D-Galp linkage	892:919	The (1 → 6)-α-D-Galp linkage was the primary chain in FSRP-1 and HSRP-1, whereas the (1 → 3)-β-D-Glcp was the backbone structure in FSRP-2 and HSRP-2.
34366479	6	53	theme	-α-D-Galp	903:911	arg1	chain					937:941	the primary chain	925:941	the primary chain	925:941	The (1 → 6)-α-D-Galp linkage was the primary chain in FSRP-1 and HSRP-1, whereas the (1 → 3)-β-D-Glcp was the backbone structure in FSRP-2 and HSRP-2.
34366479	2	54	theme	freeze-vacuum	391:403	arg1	FVD					413:415	FVD	413:415	FVD	413:415	Fresh S. rugosoannulata were processed with freeze-vacuum drying (FVD) and hot-air drying (HAD).
34366479	2	54	theme	freeze-vacuum	391:403	arg1	drying					405:410	freeze-vacuum drying	391:410	freeze-vacuum drying (FVD)	391:416	Fresh S. rugosoannulata were processed with freeze-vacuum drying (FVD) and hot-air drying (HAD).
34366479	9	55	theme	functional	1501:1510	arg1	foods					1512:1516	functional foods	1501:1516	functional foods	1501:1516	Hot-air dried S. rugosoannulata could therefore be recommended as a suitable candidate for use in the preparation of antioxidant polysaccharides as functional foods.
34366479	1	56	from	types	252:256	arg1	rugosoannulata					300:313	Stropharia rugosoannulata	289:313	Stropharia rugosoannulata	289:313	We investigated the structural characteristics and antioxidant activities of two types of neutral polysaccharides and two types of acidic polysaccharides from Stropharia rugosoannulata under different drying methods.
34366479	0	57	from	rugosoannulata	114:127	arg1	polysaccharides					82:96	polysaccharides	82:96	polysaccharides from Stropharia rugosoannulata	82:127	Effects of drying on the structural characteristics and antioxidant activities of polysaccharides from Stropharia rugosoannulata.
34366479	0	57	from	rugosoannulata	114:127	arg1	characteristics					36:50	structural characteristics	25:50	structural characteristics	25:50	Effects of drying on the structural characteristics and antioxidant activities of polysaccharides from Stropharia rugosoannulata.
34366479	0	57	from	rugosoannulata	114:127	arg1	activities					68:77	antioxidant activities	56:77	antioxidant activities	56:77	Effects of drying on the structural characteristics and antioxidant activities of polysaccharides from Stropharia rugosoannulata.
34366479	2	58	dep	drying	430:435	arg1	HAD					438:440	HAD	438:440	HAD	438:440	Fresh S. rugosoannulata were processed with freeze-vacuum drying (FVD) and hot-air drying (HAD).
34366479	3	59	theme	SRPs	577:580	arg1	types					560:564	two types	556:564	two types of neutral SRPs (FSRP-1 and HSRP-1)	556:600	Polysaccharides from the dried S. rugosoannulata (SRP) were purified using a DEAE-52 cellulose column to obtain two types of neutral SRPs (FSRP-1 and HSRP-1) and two types of acidic SRPs (FSRP-2 and HSRP-2).
34366479	3	59	theme	SRPs	577:580	arg1	types					610:614	two types	606:614	two types of acidic SRPs (FSRP-2 and HSRP-2)	606:649	Polysaccharides from the dried S. rugosoannulata (SRP) were purified using a DEAE-52 cellulose column to obtain two types of neutral SRPs (FSRP-1 and HSRP-1) and two types of acidic SRPs (FSRP-2 and HSRP-2).
34366479	1	60	theme	types	252:256	arg1	characteristics					161:175	structural characteristics	150:175	structural characteristics	150:175	We investigated the structural characteristics and antioxidant activities of two types of neutral polysaccharides and two types of acidic polysaccharides from Stropharia rugosoannulata under different drying methods.
34366479	1	60	theme	types	252:256	arg1	activities					193:202	antioxidant activities	181:202	antioxidant activities	181:202	We investigated the structural characteristics and antioxidant activities of two types of neutral polysaccharides and two types of acidic polysaccharides from Stropharia rugosoannulata under different drying methods.
34366479	9	61	theme	dried	1361:1365	arg1	candidate					1430:1438	a suitable candidate	1419:1438	a suitable candidate for use in the preparation of antioxidant polysaccharides as functional foods	1419:1516	Hot-air dried S. rugosoannulata could therefore be recommended as a suitable candidate for use in the preparation of antioxidant polysaccharides as functional foods.
34366479	9	61	theme	dried	1361:1365	arg1	rugosoannulata					1370:1383	Hot-air dried S. rugosoannulata	1353:1383	Hot-air dried S. rugosoannulata	1353:1383	Hot-air dried S. rugosoannulata could therefore be recommended as a suitable candidate for use in the preparation of antioxidant polysaccharides as functional foods.
34366479	3	62	theme	SRPs	626:629	arg1	types					560:564	two types	556:564	two types of neutral SRPs (FSRP-1 and HSRP-1)	556:600	Polysaccharides from the dried S. rugosoannulata (SRP) were purified using a DEAE-52 cellulose column to obtain two types of neutral SRPs (FSRP-1 and HSRP-1) and two types of acidic SRPs (FSRP-2 and HSRP-2).
34366479	3	62	theme	SRPs	626:629	arg1	types					610:614	two types	606:614	two types of acidic SRPs (FSRP-2 and HSRP-2)	606:649	Polysaccharides from the dried S. rugosoannulata (SRP) were purified using a DEAE-52 cellulose column to obtain two types of neutral SRPs (FSRP-1 and HSRP-1) and two types of acidic SRPs (FSRP-2 and HSRP-2).
34366479	1	63	from	types	211:215	arg1	rugosoannulata					300:313	Stropharia rugosoannulata	289:313	Stropharia rugosoannulata	289:313	We investigated the structural characteristics and antioxidant activities of two types of neutral polysaccharides and two types of acidic polysaccharides from Stropharia rugosoannulata under different drying methods.
34366479	1	64	theme	structural	150:159	arg1	characteristics					161:175	structural characteristics	150:175	structural characteristics	150:175	We investigated the structural characteristics and antioxidant activities of two types of neutral polysaccharides and two types of acidic polysaccharides from Stropharia rugosoannulata under different drying methods.
34366479	8	65	theme	antioxidant	1203:1213	arg1	power					1215:1219	positive ferric ion reducing antioxidant power	1174:1219	positive ferric ion reducing antioxidant power	1174:1219	FSRP-1, FSRP-2, HSRP-1 and HSRP-2 had positive ferric ion reducing antioxidant power and scavenging activities on ABTS+ and hydroxyl radicals, whereas HSRP exhibited a stronger antioxidant activity than that of FSRP.
34366479	3	66	dep	SRPs	577:580	arg1	HSRP-1					594:599	HSRP-1	594:599	HSRP-1	594:599	Polysaccharides from the dried S. rugosoannulata (SRP) were purified using a DEAE-52 cellulose column to obtain two types of neutral SRPs (FSRP-1 and HSRP-1) and two types of acidic SRPs (FSRP-2 and HSRP-2).
34366479	3	66	dep	SRPs	577:580	arg1	FSRP-1					583:588	FSRP-1	583:588	FSRP-1	583:588	Polysaccharides from the dried S. rugosoannulata (SRP) were purified using a DEAE-52 cellulose column to obtain two types of neutral SRPs (FSRP-1 and HSRP-1) and two types of acidic SRPs (FSRP-2 and HSRP-2).
34366479	3	66	dep	SRPs	577:580	arg1	SRPs					577:580	neutral SRPs	569:580	neutral SRPs (FSRP-1 and HSRP-1)	569:600	Polysaccharides from the dried S. rugosoannulata (SRP) were purified using a DEAE-52 cellulose column to obtain two types of neutral SRPs (FSRP-1 and HSRP-1) and two types of acidic SRPs (FSRP-2 and HSRP-2).
34366479	1	67	theme	acidic	261:266	arg1	polysaccharides					268:282	acidic polysaccharides	261:282	acidic polysaccharides from Stropharia rugosoannulata	261:313	We investigated the structural characteristics and antioxidant activities of two types of neutral polysaccharides and two types of acidic polysaccharides from Stropharia rugosoannulata under different drying methods.
34366479	0	68	theme	antioxidant	56:66	arg1	activities					68:77	antioxidant activities	56:77	antioxidant activities	56:77	Effects of drying on the structural characteristics and antioxidant activities of polysaccharides from Stropharia rugosoannulata.
34366479	6	69	theme	1 → 6	897:901	arg1	linkage					913:919	The (1 → 6)-α-D-Galp linkage	892:919	The (1 → 6)-α-D-Galp linkage	892:919	The (1 → 6)-α-D-Galp linkage was the primary chain in FSRP-1 and HSRP-1, whereas the (1 → 3)-β-D-Glcp was the backbone structure in FSRP-2 and HSRP-2.
34366479	6	69	theme	1 → 6	897:901	arg1	chain					937:941	the primary chain	925:941	the primary chain	925:941	The (1 → 6)-α-D-Galp linkage was the primary chain in FSRP-1 and HSRP-1, whereas the (1 → 3)-β-D-Glcp was the backbone structure in FSRP-2 and HSRP-2.
34366479	6	70	theme	1 → 3	978:982	arg1	structure					1011:1019	the backbone structure	998:1019	the backbone structure in FSRP-2 and HSRP-2	998:1040	The (1 → 6)-α-D-Galp linkage was the primary chain in FSRP-1 and HSRP-1, whereas the (1 → 3)-β-D-Glcp was the backbone structure in FSRP-2 and HSRP-2.
34366479	6	70	theme	1 → 3	978:982	arg1	-β-D-Glcp					984:992	the (1 → 3)-β-D-Glcp	973:992	the (1 → 3)-β-D-Glcp	973:992	The (1 → 6)-α-D-Galp linkage was the primary chain in FSRP-1 and HSRP-1, whereas the (1 → 3)-β-D-Glcp was the backbone structure in FSRP-2 and HSRP-2.
34366479	8	71	theme	scavenging	1225:1234	arg1	activities					1236:1245	scavenging activities	1225:1245	scavenging activities	1225:1245	FSRP-1, FSRP-2, HSRP-1 and HSRP-2 had positive ferric ion reducing antioxidant power and scavenging activities on ABTS+ and hydroxyl radicals, whereas HSRP exhibited a stronger antioxidant activity than that of FSRP.
34366479	1	72	theme	polysaccharides	268:282	arg1	types					211:215	two types	207:215	two types of neutral polysaccharides	207:242	We investigated the structural characteristics and antioxidant activities of two types of neutral polysaccharides and two types of acidic polysaccharides from Stropharia rugosoannulata under different drying methods.
34366479	1	72	theme	polysaccharides	268:282	arg1	types					252:256	two types	248:256	two types of acidic polysaccharides from Stropharia rugosoannulata	248:313	We investigated the structural characteristics and antioxidant activities of two types of neutral polysaccharides and two types of acidic polysaccharides from Stropharia rugosoannulata under different drying methods.
32650568	16	0	theme	fecal	2171:2175	arg1	microbiome					2177:2186	the fecal microbiome	2167:2186	the fecal microbiome	2167:2186	Prebiotic supplementation with OMNi-LOGiC® FIBRE seemed to induce modifications of the fecal microbiome and VOC profile but did not improve gut permeability.
32650568	13	1	theme	higher	1761:1766	arg1	permeability					1772:1783	significantly higher gut permeability	1747:1783	significantly higher gut permeability	1747:1783	NB animals had significantly higher gut permeability than Aqua animals; POS did not ameliorate these changes.
32650568	2	2	theme	positive	328:335	arg1	effects					347:353	positive metabolic effects	328:353	positive metabolic effects	328:353	Supplementation with prebiotic non-digestible carbohydrates exerts positive metabolic effects in experimental oncologic diseases.
32650568	3	3	with	supplementation	453:467	arg1	FIBRE					486:490	FIBRE	486:490	FIBRE	486:490	The aim of this project was to assess the effect of prebiotic supplementation with OMNi-LOGiC® FIBRE on intestinal microbiome, bacterial metabolism, gut permeability, and inflammation in a murine model of neuroblastoma (NB)-associated TAC.
32650568	16	4	theme	profile	2196:2202	arg1	modifications					2150:2162	modifications	2150:2162	modifications of the fecal microbiome and VOC profile	2150:2202	Prebiotic supplementation with OMNi-LOGiC® FIBRE seemed to induce modifications of the fecal microbiome and VOC profile but did not improve gut permeability.
32650568	4	5	dep	NB-Aqua	816:822	arg1	n					841:841	n = 12	841:846	n = 12	841:846	For this study, 2,000,000 NB cells (MHH-NB11) were implanted into athymic mice followed by daily supplementation with water or 200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC® FIBRE (NB-Aqua, n = 12; NB-POS, n = 12).
32650568	4	5	dep	NB-Aqua	816:822	arg1	NB-POS					833:838	NB-POS	833:838	NB-POS	833:838	For this study, 2,000,000 NB cells (MHH-NB11) were implanted into athymic mice followed by daily supplementation with water or 200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC® FIBRE (NB-Aqua, n = 12; NB-POS, n = 12).
32650568	4	5	dep	NB-Aqua	816:822	arg1	n					825:825	n	825:825	n = 12	825:830	For this study, 2,000,000 NB cells (MHH-NB11) were implanted into athymic mice followed by daily supplementation with water or 200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC® FIBRE (NB-Aqua, n = 12; NB-POS, n = 12).
32650568	4	6	theme	=	843:843	arg1	n					841:841	n = 12	841:846	n = 12	841:846	For this study, 2,000,000 NB cells (MHH-NB11) were implanted into athymic mice followed by daily supplementation with water or 200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC® FIBRE (NB-Aqua, n = 12; NB-POS, n = 12).
32650568	4	7	theme	athymic	697:703	arg1	mice					705:708	athymic mice	697:708	athymic mice followed by daily supplementation with water or 200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC® FIBRE (NB-Aqua, n = 12; NB-POS, n = 12)	697:847	For this study, 2,000,000 NB cells (MHH-NB11) were implanted into athymic mice followed by daily supplementation with water or 200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC® FIBRE (NB-Aqua, n = 12; NB-POS, n = 12).
32650568	11	8	theme	taxonomic	1619:1627	arg1	alterations					1629:1639	taxonomic alterations	1619:1639	taxonomic alterations of the fecal microbiome	1619:1663	Both NB and POS supplementation led to taxonomic alterations of the fecal microbiome.
32650568	13	9	theme	gut	1768:1770	arg1	permeability					1772:1783	significantly higher gut permeability	1747:1783	significantly higher gut permeability	1747:1783	NB animals had significantly higher gut permeability than Aqua animals; POS did not ameliorate these changes.
32650568	2	10	theme	experimental	358:369	arg1	diseases					381:388	experimental oncologic diseases	358:388	experimental oncologic diseases	358:388	Supplementation with prebiotic non-digestible carbohydrates exerts positive metabolic effects in experimental oncologic diseases.
32650568	6	11	theme	tumor	923:927	arg1	growth					929:934	tumor growth	923:934	tumor growth (first visibility to euthanasia)	923:967	The median time of tumor growth (first visibility to euthanasia) was 37 days (IQR 12.5 days) in the NB-Aqua group and 37 days (IQR 36.5 days) in the NB-POS group (p = 0.791).
32650568	11	12	theme	fecal	1648:1652	arg1	microbiome					1654:1663	the fecal microbiome	1644:1663	the fecal microbiome	1644:1663	Both NB and POS supplementation led to taxonomic alterations of the fecal microbiome.
32650568	0	13	theme	Gut	123:125	arg1	Permeability					127:138	Gut Permeability	123:138	Gut Permeability	123:138	The Effects of Prebiotic Supplementation with OMNi-LOGiC® FIBRE on Fecal Microbiome, Fecal Volatile Organic Compounds, and Gut Permeability in Murine Neuroblastoma-Induced Tumor-Associated Cachexia.
32650568	2	14	with	Supplementation	261:275	arg1	carbohydrates					307:319	prebiotic non-digestible carbohydrates	282:319	prebiotic non-digestible carbohydrates	282:319	Supplementation with prebiotic non-digestible carbohydrates exerts positive metabolic effects in experimental oncologic diseases.
32650568	8	15	theme	culture	1318:1324	arg1	medium					1326:1331	culture medium	1318:1331	culture medium	1318:1331	Values were compared to sham animals following injection of culture medium and gavage of either water or OMNi-LOGiC® FIBRE (SH-Aqua, n = 10; SH-POS, n = 10).
32650568	7	16	theme	barrier	1227:1233	arg1	markers					1235:1241	gut barrier markers	1223:1241	gut barrier markers	1223:1241	At euthanasia, fecal microbiome and volatile organic compounds (VOCs), gut permeability (fluorescein isothiocyanate-dextran (FITC-dextran), and gut barrier markers were measured.
32650568	13	17	theme	NB	1732:1733	arg1	animals					1735:1741	NB animals	1732:1741	NB animals	1732:1741	NB animals had significantly higher gut permeability than Aqua animals; POS did not ameliorate these changes.
32650568	0	18	theme	Neuroblastoma-Induced	150:170	arg1	Cachexia					189:196	Murine Neuroblastoma-Induced Tumor-Associated Cachexia	143:196	Murine Neuroblastoma-Induced Tumor-Associated Cachexia	143:196	The Effects of Prebiotic Supplementation with OMNi-LOGiC® FIBRE on Fecal Microbiome, Fecal Volatile Organic Compounds, and Gut Permeability in Murine Neuroblastoma-Induced Tumor-Associated Cachexia.
32650568	2	19	theme	prebiotic	282:290	arg1	carbohydrates					307:319	prebiotic non-digestible carbohydrates	282:319	prebiotic non-digestible carbohydrates	282:319	Supplementation with prebiotic non-digestible carbohydrates exerts positive metabolic effects in experimental oncologic diseases.
32650568	7	20	theme	fecal	1094:1098	arg1	microbiome					1100:1109	fecal microbiome	1094:1109	fecal microbiome	1094:1109	At euthanasia, fecal microbiome and volatile organic compounds (VOCs), gut permeability (fluorescein isothiocyanate-dextran (FITC-dextran), and gut barrier markers were measured.
32650568	7	20	theme	fecal	1094:1098	arg1	VOCs					1143:1146	VOCs	1143:1146	VOCs	1143:1146	At euthanasia, fecal microbiome and volatile organic compounds (VOCs), gut permeability (fluorescein isothiocyanate-dextran (FITC-dextran), and gut barrier markers were measured.
32650568	8	21	theme	water	1354:1358	arg1	gavage					1337:1342	gavage	1337:1342	gavage of either water or OMNi-LOGiC® FIBRE (SH-Aqua, n = 10; SH-POS, n = 10)	1337:1413	Values were compared to sham animals following injection of culture medium and gavage of either water or OMNi-LOGiC® FIBRE (SH-Aqua, n = 10; SH-POS, n = 10).
32650568	8	21	theme	water	1354:1358	arg1	injection					1305:1313	injection	1305:1313	injection of culture medium	1305:1331	Values were compared to sham animals following injection of culture medium and gavage of either water or OMNi-LOGiC® FIBRE (SH-Aqua, n = 10; SH-POS, n = 10).
32650568	4	22	theme	daily	722:726	arg1	supplementation					728:742	daily supplementation	722:742	daily supplementation with water or 200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC® FIBRE (NB-Aqua, n = 12; NB-POS, n = 12)	722:847	For this study, 2,000,000 NB cells (MHH-NB11) were implanted into athymic mice followed by daily supplementation with water or 200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC® FIBRE (NB-Aqua, n = 12; NB-POS, n = 12).
32650568	5	23	theme	tumor	872:876	arg1	group					878:882	each tumor group	867:882	each tumor group	867:882	Three animals of each tumor group did not develop NB.
32650568	3	24	theme	project	407:413	arg1	aim					395:397	The aim	391:397	The aim of this project	391:413	The aim of this project was to assess the effect of prebiotic supplementation with OMNi-LOGiC® FIBRE on intestinal microbiome, bacterial metabolism, gut permeability, and inflammation in a murine model of neuroblastoma (NB)-associated TAC.
32650568	0	25	theme	Fecal	85:89	arg1	Compounds					108:116	Fecal Volatile Organic Compounds	85:116	Fecal Volatile Organic Compounds	85:116	The Effects of Prebiotic Supplementation with OMNi-LOGiC® FIBRE on Fecal Microbiome, Fecal Volatile Organic Compounds, and Gut Permeability in Murine Neuroblastoma-Induced Tumor-Associated Cachexia.
32650568	0	26	theme	Organic	100:106	arg1	Compounds					108:116	Fecal Volatile Organic Compounds	85:116	Fecal Volatile Organic Compounds	85:116	The Effects of Prebiotic Supplementation with OMNi-LOGiC® FIBRE on Fecal Microbiome, Fecal Volatile Organic Compounds, and Gut Permeability in Murine Neuroblastoma-Induced Tumor-Associated Cachexia.
32650568	15	27	from	changes	2035:2041	arg1	microbiome					2056:2065	the fecal microbiome	2046:2065	the fecal microbiome	2046:2065	In conclusion, our results suggest that NB-induced TAC causes increased gut permeability coupled with compositional changes in the fecal microbiome and VOC profile.
32650568	15	27	from	changes	2035:2041	arg1	profile					2075:2081	VOC profile	2071:2081	VOC profile	2071:2081	In conclusion, our results suggest that NB-induced TAC causes increased gut permeability coupled with compositional changes in the fecal microbiome and VOC profile.
32650568	4	28	theme	mg	762:763	arg1	OMNi-LOGiC®					797:807	200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC®	758:807	200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC®	758:807	For this study, 2,000,000 NB cells (MHH-NB11) were implanted into athymic mice followed by daily supplementation with water or 200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC® FIBRE (NB-Aqua, n = 12; NB-POS, n = 12).
32650568	4	29	dep	water	749:753	arg1	NB-Aqua					816:822	NB-Aqua	816:822	NB-Aqua	816:822	For this study, 2,000,000 NB cells (MHH-NB11) were implanted into athymic mice followed by daily supplementation with water or 200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC® FIBRE (NB-Aqua, n = 12; NB-POS, n = 12).
32650568	4	29	dep	water	749:753	arg1	FIBRE					809:813	FIBRE	809:813	FIBRE	809:813	For this study, 2,000,000 NB cells (MHH-NB11) were implanted into athymic mice followed by daily supplementation with water or 200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC® FIBRE (NB-Aqua, n = 12; NB-POS, n = 12).
32650568	8	30	theme	=	1393:1393	arg1	n					1391:1391	n = 10	1391:1396	n = 10	1391:1396	Values were compared to sham animals following injection of culture medium and gavage of either water or OMNi-LOGiC® FIBRE (SH-Aqua, n = 10; SH-POS, n = 10).
32650568	4	31	theme	oligosaccharide	775:789	arg1	OMNi-LOGiC®					797:807	200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC®	758:807	200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC®	758:807	For this study, 2,000,000 NB cells (MHH-NB11) were implanted into athymic mice followed by daily supplementation with water or 200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC® FIBRE (NB-Aqua, n = 12; NB-POS, n = 12).
32650568	3	32	theme	bacterial	518:526	arg1	metabolism					528:537	bacterial metabolism	518:537	bacterial metabolism	518:537	The aim of this project was to assess the effect of prebiotic supplementation with OMNi-LOGiC® FIBRE on intestinal microbiome, bacterial metabolism, gut permeability, and inflammation in a murine model of neuroblastoma (NB)-associated TAC.
32650568	3	33	theme	supplementation	453:467	arg1	effect					433:438	the effect	429:438	the effect of prebiotic supplementation with OMNi-LOGiC® FIBRE on intestinal microbiome, bacterial metabolism, gut permeability, and inflammation	429:573	The aim of this project was to assess the effect of prebiotic supplementation with OMNi-LOGiC® FIBRE on intestinal microbiome, bacterial metabolism, gut permeability, and inflammation in a murine model of neuroblastoma (NB)-associated TAC.
32650568	14	34	theme	tight	1872:1876	arg1	junctions					1878:1886	tight junctions	1872:1886	tight junctions	1872:1886	The pore and leak pathways of tight junctions did not differ between groups.
32650568	4	35	theme	=	827:827	arg1	n					825:825	n	825:825	n = 12	825:830	For this study, 2,000,000 NB cells (MHH-NB11) were implanted into athymic mice followed by daily supplementation with water or 200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC® FIBRE (NB-Aqua, n = 12; NB-POS, n = 12).
32650568	16	36	theme	Prebiotic	2084:2092	arg1	supplementation					2094:2108	Prebiotic supplementation	2084:2108	Prebiotic supplementation with OMNi-LOGiC® FIBRE	2084:2131	Prebiotic supplementation with OMNi-LOGiC® FIBRE seemed to induce modifications of the fecal microbiome and VOC profile but did not improve gut permeability.
32650568	10	37	theme	coordinate	1491:1500	arg1	PCoA					1512:1515	PCoA	1512:1515	PCoA	1512:1515	Principal coordinate analysis (PCoA) revealed clustering differences between Aqua and POS animals.
32650568	10	37	theme	coordinate	1491:1500	arg1	analysis					1502:1509	Principal coordinate analysis	1481:1509	Principal coordinate analysis (PCoA)	1481:1516	Principal coordinate analysis (PCoA) revealed clustering differences between Aqua and POS animals.
32650568	0	38	theme	Supplementation	25:39	arg1	Effects					4:10	The Effects	0:10	The Effects of Prebiotic Supplementation with OMNi-LOGiC® FIBRE on Fecal Microbiome, Fecal Volatile Organic Compounds, and Gut Permeability	0:138	The Effects of Prebiotic Supplementation with OMNi-LOGiC® FIBRE on Fecal Microbiome, Fecal Volatile Organic Compounds, and Gut Permeability in Murine Neuroblastoma-Induced Tumor-Associated Cachexia.
32650568	7	39	dep	isothiocyanate-dextran	1180:1201	arg1	FITC-dextran					1204:1215	FITC-dextran	1204:1215	FITC-dextran	1204:1215	At euthanasia, fecal microbiome and volatile organic compounds (VOCs), gut permeability (fluorescein isothiocyanate-dextran (FITC-dextran), and gut barrier markers were measured.
32650568	7	40	theme	gut	1150:1152	arg1	permeability					1154:1165	gut permeability	1150:1165	gut permeability (fluorescein isothiocyanate-dextran (FITC-dextran)	1150:1216	At euthanasia, fecal microbiome and volatile organic compounds (VOCs), gut permeability (fluorescein isothiocyanate-dextran (FITC-dextran), and gut barrier markers were measured.
32650568	7	40	theme	gut	1150:1152	arg1	isothiocyanate-dextran					1180:1201	fluorescein isothiocyanate-dextran	1168:1201	fluorescein isothiocyanate-dextran	1168:1201	At euthanasia, fecal microbiome and volatile organic compounds (VOCs), gut permeability (fluorescein isothiocyanate-dextran (FITC-dextran), and gut barrier markers were measured.
32650568	6	41	from	days	1025:1028	arg1	group					1060:1064	the NB-POS group	1049:1064	the NB-POS group (p = 0.791)	1049:1076	The median time of tumor growth (first visibility to euthanasia) was 37 days (IQR 12.5 days) in the NB-Aqua group and 37 days (IQR 36.5 days) in the NB-POS group (p = 0.791).
32650568	6	41	from	days	1025:1028	arg1	group					1012:1016	the NB-Aqua group	1000:1016	the NB-Aqua group	1000:1016	The median time of tumor growth (first visibility to euthanasia) was 37 days (IQR 12.5 days) in the NB-Aqua group and 37 days (IQR 36.5 days) in the NB-POS group (p = 0.791).
32650568	6	41	from	days	1025:1028	arg1	p					1067:1067	p = 0.791	1067:1075	p = 0.791	1067:1075	The median time of tumor growth (first visibility to euthanasia) was 37 days (IQR 12.5 days) in the NB-Aqua group and 37 days (IQR 36.5 days) in the NB-POS group (p = 0.791).
32650568	10	42	theme	clustering	1527:1536	arg1	differences					1538:1548	clustering differences	1527:1548	clustering differences between Aqua and POS animals	1527:1577	Principal coordinate analysis (PCoA) revealed clustering differences between Aqua and POS animals.
32650568	15	43	theme	fecal	2050:2054	arg1	microbiome					2056:2065	the fecal microbiome	2046:2065	the fecal microbiome	2046:2065	In conclusion, our results suggest that NB-induced TAC causes increased gut permeability coupled with compositional changes in the fecal microbiome and VOC profile.
32650568	8	44	theme	=	1409:1409	arg1	n					1407:1407	n = 10	1407:1412	n = 10	1407:1412	Values were compared to sham animals following injection of culture medium and gavage of either water or OMNi-LOGiC® FIBRE (SH-Aqua, n = 10; SH-POS, n = 10).
32650568	8	45	theme	sham	1282:1285	arg1	animals					1287:1293	sham animals	1282:1293	sham animals	1282:1293	Values were compared to sham animals following injection of culture medium and gavage of either water or OMNi-LOGiC® FIBRE (SH-Aqua, n = 10; SH-POS, n = 10).
32650568	16	46	theme	gut	2224:2226	arg1	permeability					2228:2239	gut permeability	2224:2239	gut permeability	2224:2239	Prebiotic supplementation with OMNi-LOGiC® FIBRE seemed to induce modifications of the fecal microbiome and VOC profile but did not improve gut permeability.
32650568	15	47	theme	increased	1981:1989	arg1	permeability					1995:2006	increased gut permeability	1981:2006	increased gut permeability coupled with compositional changes in the fecal microbiome and VOC profile	1981:2081	In conclusion, our results suggest that NB-induced TAC causes increased gut permeability coupled with compositional changes in the fecal microbiome and VOC profile.
32650568	8	48	dep	SH-Aqua	1382:1388	arg1	n					1407:1407	n = 10	1407:1412	n = 10	1407:1412	Values were compared to sham animals following injection of culture medium and gavage of either water or OMNi-LOGiC® FIBRE (SH-Aqua, n = 10; SH-POS, n = 10).
32650568	8	48	dep	SH-Aqua	1382:1388	arg1	SH-POS					1399:1404	SH-POS	1399:1404	SH-POS	1399:1404	Values were compared to sham animals following injection of culture medium and gavage of either water or OMNi-LOGiC® FIBRE (SH-Aqua, n = 10; SH-POS, n = 10).
32650568	8	48	dep	SH-Aqua	1382:1388	arg1	n					1391:1391	n = 10	1391:1396	n = 10	1391:1396	Values were compared to sham animals following injection of culture medium and gavage of either water or OMNi-LOGiC® FIBRE (SH-Aqua, n = 10; SH-POS, n = 10).
32650568	7	49	theme	organic	1124:1130	arg1	compounds					1132:1140	volatile organic compounds	1115:1140	volatile organic compounds	1115:1140	At euthanasia, fecal microbiome and volatile organic compounds (VOCs), gut permeability (fluorescein isothiocyanate-dextran (FITC-dextran), and gut barrier markers were measured.
32650568	4	50	theme	2,000,000	647:655	arg1	MHH-NB11					667:674	MHH-NB11	667:674	MHH-NB11	667:674	For this study, 2,000,000 NB cells (MHH-NB11) were implanted into athymic mice followed by daily supplementation with water or 200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC® FIBRE (NB-Aqua, n = 12; NB-POS, n = 12).
32650568	4	50	theme	2,000,000	647:655	arg1	cells					660:664	2,000,000 NB cells	647:664	2,000,000 NB cells (MHH-NB11)	647:675	For this study, 2,000,000 NB cells (MHH-NB11) were implanted into athymic mice followed by daily supplementation with water or 200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC® FIBRE (NB-Aqua, n = 12; NB-POS, n = 12).
32650568	0	51	with	Supplementation	25:39	arg1	FIBRE					58:62	FIBRE	58:62	FIBRE	58:62	The Effects of Prebiotic Supplementation with OMNi-LOGiC® FIBRE on Fecal Microbiome, Fecal Volatile Organic Compounds, and Gut Permeability in Murine Neuroblastoma-Induced Tumor-Associated Cachexia.
32650568	6	52	theme	NB-Aqua	1004:1010	arg1	group					1012:1016	the NB-Aqua group	1000:1016	the NB-Aqua group	1000:1016	The median time of tumor growth (first visibility to euthanasia) was 37 days (IQR 12.5 days) in the NB-Aqua group and 37 days (IQR 36.5 days) in the NB-POS group (p = 0.791).
32650568	4	53	with	supplementation	728:742	arg1	water					749:753	water	749:753	water	749:753	For this study, 2,000,000 NB cells (MHH-NB11) were implanted into athymic mice followed by daily supplementation with water or 200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC® FIBRE (NB-Aqua, n = 12; NB-POS, n = 12).
32650568	4	53	with	supplementation	728:742	arg1	OMNi-LOGiC®					797:807	200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC®	758:807	200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC®	758:807	For this study, 2,000,000 NB cells (MHH-NB11) were implanted into athymic mice followed by daily supplementation with water or 200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC® FIBRE (NB-Aqua, n = 12; NB-POS, n = 12).
32650568	13	54	theme	Aqua	1790:1793	arg1	animals					1795:1801	Aqua animals	1790:1801	Aqua animals	1790:1801	NB animals had significantly higher gut permeability than Aqua animals; POS did not ameliorate these changes.
32650568	2	55	theme	metabolic	337:345	arg1	effects					347:353	positive metabolic effects	328:353	positive metabolic effects	328:353	Supplementation with prebiotic non-digestible carbohydrates exerts positive metabolic effects in experimental oncologic diseases.
32650568	16	56	theme	microbiome	2177:2186	arg1	modifications					2150:2162	modifications	2150:2162	modifications of the fecal microbiome and VOC profile	2150:2202	Prebiotic supplementation with OMNi-LOGiC® FIBRE seemed to induce modifications of the fecal microbiome and VOC profile but did not improve gut permeability.
32650568	3	57	theme	murine	580:585	arg1	model					587:591	a murine model	578:591	a murine model of neuroblastoma (NB)-associated TAC	578:628	The aim of this project was to assess the effect of prebiotic supplementation with OMNi-LOGiC® FIBRE on intestinal microbiome, bacterial metabolism, gut permeability, and inflammation in a murine model of neuroblastoma (NB)-associated TAC.
32650568	15	58	theme	NB-induced	1959:1968	arg1	TAC					1970:1972	NB-induced TAC	1959:1972	NB-induced TAC	1959:1972	In conclusion, our results suggest that NB-induced TAC causes increased gut permeability coupled with compositional changes in the fecal microbiome and VOC profile.
32650568	6	59	theme	NB-POS	1053:1058	arg1	group					1060:1064	the NB-POS group	1049:1064	the NB-POS group (p = 0.791)	1049:1076	The median time of tumor growth (first visibility to euthanasia) was 37 days (IQR 12.5 days) in the NB-Aqua group and 37 days (IQR 36.5 days) in the NB-POS group (p = 0.791).
32650568	6	59	theme	NB-POS	1053:1058	arg1	p					1067:1067	p = 0.791	1067:1075	p = 0.791	1067:1075	The median time of tumor growth (first visibility to euthanasia) was 37 days (IQR 12.5 days) in the NB-Aqua group and 37 days (IQR 36.5 days) in the NB-POS group (p = 0.791).
32650568	6	60	from	days	976:979	arg1	group					1060:1064	the NB-POS group	1049:1064	the NB-POS group (p = 0.791)	1049:1076	The median time of tumor growth (first visibility to euthanasia) was 37 days (IQR 12.5 days) in the NB-Aqua group and 37 days (IQR 36.5 days) in the NB-POS group (p = 0.791).
32650568	6	60	from	days	976:979	arg1	group					1012:1016	the NB-Aqua group	1000:1016	the NB-Aqua group	1000:1016	The median time of tumor growth (first visibility to euthanasia) was 37 days (IQR 12.5 days) in the NB-Aqua group and 37 days (IQR 36.5 days) in the NB-POS group (p = 0.791).
32650568	6	60	from	days	976:979	arg1	p					1067:1067	p = 0.791	1067:1075	p = 0.791	1067:1075	The median time of tumor growth (first visibility to euthanasia) was 37 days (IQR 12.5 days) in the NB-Aqua group and 37 days (IQR 36.5 days) in the NB-POS group (p = 0.791).
32650568	2	61	theme	oncologic	371:379	arg1	diseases					381:388	experimental oncologic diseases	358:388	experimental oncologic diseases	358:388	Supplementation with prebiotic non-digestible carbohydrates exerts positive metabolic effects in experimental oncologic diseases.
32650568	1	62	theme	Malignant	199:207	arg1	diseases					209:216	Malignant diseases	199:216	Malignant diseases	199:216	Malignant diseases can cause tumor-associated cachexia (TAC).
32650568	8	63	theme	FIBRE	1375:1379	arg1	gavage					1337:1342	gavage	1337:1342	gavage of either water or OMNi-LOGiC® FIBRE (SH-Aqua, n = 10; SH-POS, n = 10)	1337:1413	Values were compared to sham animals following injection of culture medium and gavage of either water or OMNi-LOGiC® FIBRE (SH-Aqua, n = 10; SH-POS, n = 10).
32650568	8	63	theme	FIBRE	1375:1379	arg1	injection					1305:1313	injection	1305:1313	injection of culture medium	1305:1331	Values were compared to sham animals following injection of culture medium and gavage of either water or OMNi-LOGiC® FIBRE (SH-Aqua, n = 10; SH-POS, n = 10).
32650568	6	64	theme	growth	929:934	arg1	days					976:979	37 days	973:979	37 days (IQR 12.5 days) in the NB-Aqua group	973:1016	The median time of tumor growth (first visibility to euthanasia) was 37 days (IQR 12.5 days) in the NB-Aqua group and 37 days (IQR 36.5 days) in the NB-POS group (p = 0.791).
32650568	6	64	theme	growth	929:934	arg1	time					915:918	The median time	904:918	The median time of tumor growth (first visibility to euthanasia)	904:967	The median time of tumor growth (first visibility to euthanasia) was 37 days (IQR 12.5 days) in the NB-Aqua group and 37 days (IQR 36.5 days) in the NB-POS group (p = 0.791).
32650568	3	65	theme	-associated	614:624	arg1	TAC					626:628	neuroblastoma (NB)-associated TAC	596:628	neuroblastoma (NB)-associated TAC	596:628	The aim of this project was to assess the effect of prebiotic supplementation with OMNi-LOGiC® FIBRE on intestinal microbiome, bacterial metabolism, gut permeability, and inflammation in a murine model of neuroblastoma (NB)-associated TAC.
32650568	14	66	dep	pore	1846:1849	arg1	pathways					1860:1867	pathways	1860:1867	pathways	1860:1867	The pore and leak pathways of tight junctions did not differ between groups.
32650568	14	66	dep	pore	1846:1849	arg1	The					1842:1844	The	1842:1844	The	1842:1844	The pore and leak pathways of tight junctions did not differ between groups.
32650568	4	67	dep	=	827:827	arg1	12					829:830	12	829:830	12	829:830	For this study, 2,000,000 NB cells (MHH-NB11) were implanted into athymic mice followed by daily supplementation with water or 200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC® FIBRE (NB-Aqua, n = 12; NB-POS, n = 12).
32650568	6	68	theme	median	908:913	arg1	days					976:979	37 days	973:979	37 days (IQR 12.5 days) in the NB-Aqua group	973:1016	The median time of tumor growth (first visibility to euthanasia) was 37 days (IQR 12.5 days) in the NB-Aqua group and 37 days (IQR 36.5 days) in the NB-POS group (p = 0.791).
32650568	6	68	theme	median	908:913	arg1	time					915:918	The median time	904:918	The median time of tumor growth (first visibility to euthanasia)	904:967	The median time of tumor growth (first visibility to euthanasia) was 37 days (IQR 12.5 days) in the NB-Aqua group and 37 days (IQR 36.5 days) in the NB-POS group (p = 0.791).
32650568	8	69	theme	medium	1326:1331	arg1	gavage					1337:1342	gavage	1337:1342	gavage of either water or OMNi-LOGiC® FIBRE (SH-Aqua, n = 10; SH-POS, n = 10)	1337:1413	Values were compared to sham animals following injection of culture medium and gavage of either water or OMNi-LOGiC® FIBRE (SH-Aqua, n = 10; SH-POS, n = 10).
32650568	8	69	theme	medium	1326:1331	arg1	injection					1305:1313	injection	1305:1313	injection of culture medium	1305:1331	Values were compared to sham animals following injection of culture medium and gavage of either water or OMNi-LOGiC® FIBRE (SH-Aqua, n = 10; SH-POS, n = 10).
32650568	16	70	theme	VOC	2192:2194	arg1	profile					2196:2202	VOC profile	2192:2202	VOC profile	2192:2202	Prebiotic supplementation with OMNi-LOGiC® FIBRE seemed to induce modifications of the fecal microbiome and VOC profile but did not improve gut permeability.
32650568	11	71	theme	microbiome	1654:1663	arg1	alterations					1629:1639	taxonomic alterations	1619:1639	taxonomic alterations of the fecal microbiome	1619:1663	Both NB and POS supplementation led to taxonomic alterations of the fecal microbiome.
32650568	0	72	theme	Murine	143:148	arg1	Cachexia					189:196	Murine Neuroblastoma-Induced Tumor-Associated Cachexia	143:196	Murine Neuroblastoma-Induced Tumor-Associated Cachexia	143:196	The Effects of Prebiotic Supplementation with OMNi-LOGiC® FIBRE on Fecal Microbiome, Fecal Volatile Organic Compounds, and Gut Permeability in Murine Neuroblastoma-Induced Tumor-Associated Cachexia.
32650568	3	73	theme	TAC	626:628	arg1	model					587:591	a murine model	578:591	a murine model of neuroblastoma (NB)-associated TAC	578:628	The aim of this project was to assess the effect of prebiotic supplementation with OMNi-LOGiC® FIBRE on intestinal microbiome, bacterial metabolism, gut permeability, and inflammation in a murine model of neuroblastoma (NB)-associated TAC.
32650568	5	74	theme	group	878:882	arg1	animals					856:862	Three animals	850:862	Three animals of each tumor group	850:882	Three animals of each tumor group did not develop NB.
32650568	0	75	theme	Tumor-Associated	172:187	arg1	Cachexia					189:196	Murine Neuroblastoma-Induced Tumor-Associated Cachexia	143:196	Murine Neuroblastoma-Induced Tumor-Associated Cachexia	143:196	The Effects of Prebiotic Supplementation with OMNi-LOGiC® FIBRE on Fecal Microbiome, Fecal Volatile Organic Compounds, and Gut Permeability in Murine Neuroblastoma-Induced Tumor-Associated Cachexia.
32650568	2	76	theme	non-digestible	292:305	arg1	carbohydrates					307:319	prebiotic non-digestible carbohydrates	282:319	prebiotic non-digestible carbohydrates	282:319	Supplementation with prebiotic non-digestible carbohydrates exerts positive metabolic effects in experimental oncologic diseases.
32650568	15	77	theme	compositional	2021:2033	arg1	changes					2035:2041	compositional changes	2021:2041	compositional changes in the fecal microbiome and VOC profile	2021:2081	In conclusion, our results suggest that NB-induced TAC causes increased gut permeability coupled with compositional changes in the fecal microbiome and VOC profile.
32650568	6	78	dep	growth	929:934	arg1	visibility					943:952	first visibility	937:952	first visibility to euthanasia	937:966	The median time of tumor growth (first visibility to euthanasia) was 37 days (IQR 12.5 days) in the NB-Aqua group and 37 days (IQR 36.5 days) in the NB-POS group (p = 0.791).
32650568	7	79	theme	gut	1223:1225	arg1	markers					1235:1241	gut barrier markers	1223:1241	gut barrier markers	1223:1241	At euthanasia, fecal microbiome and volatile organic compounds (VOCs), gut permeability (fluorescein isothiocyanate-dextran (FITC-dextran), and gut barrier markers were measured.
32650568	0	80	theme	Fecal	67:71	arg1	Microbiome					73:82	Fecal Microbiome	67:82	Fecal Microbiome	67:82	The Effects of Prebiotic Supplementation with OMNi-LOGiC® FIBRE on Fecal Microbiome, Fecal Volatile Organic Compounds, and Gut Permeability in Murine Neuroblastoma-Induced Tumor-Associated Cachexia.
32650568	11	81	dep	supplementation	1596:1610	arg1	POS					1592:1594	POS supplementation	1592:1610	POS supplementation	1592:1610	Both NB and POS supplementation led to taxonomic alterations of the fecal microbiome.
32650568	4	82	dep	OMNi-LOGiC®	797:807	arg1	POS					792:794	200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC®	758:807	200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC®	758:807	For this study, 2,000,000 NB cells (MHH-NB11) were implanted into athymic mice followed by daily supplementation with water or 200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC® FIBRE (NB-Aqua, n = 12; NB-POS, n = 12).
32650568	13	83	contain	had	1743:1745	arg2	permeability					1772:1783	significantly higher gut permeability	1747:1783	significantly higher gut permeability	1747:1783	NB animals had significantly higher gut permeability than Aqua animals; POS did not ameliorate these changes.
32650568	13	83	contain	had	1743:1745	arg1	animals					1735:1741	NB animals	1732:1741	NB animals	1732:1741	NB animals had significantly higher gut permeability than Aqua animals; POS did not ameliorate these changes.
32650568	6	84	theme	=	1069:1069	arg1	group					1060:1064	the NB-POS group	1049:1064	the NB-POS group (p = 0.791)	1049:1076	The median time of tumor growth (first visibility to euthanasia) was 37 days (IQR 12.5 days) in the NB-Aqua group and 37 days (IQR 36.5 days) in the NB-POS group (p = 0.791).
32650568	6	84	theme	=	1069:1069	arg1	p					1067:1067	p = 0.791	1067:1075	p = 0.791	1067:1075	The median time of tumor growth (first visibility to euthanasia) was 37 days (IQR 12.5 days) in the NB-Aqua group and 37 days (IQR 36.5 days) in the NB-POS group (p = 0.791).
32650568	15	85	theme	gut	1991:1993	arg1	permeability					1995:2006	increased gut permeability	1981:2006	increased gut permeability coupled with compositional changes in the fecal microbiome and VOC profile	1981:2081	In conclusion, our results suggest that NB-induced TAC causes increased gut permeability coupled with compositional changes in the fecal microbiome and VOC profile.
32650568	0	86	from	Effects	4:10	arg1	Microbiome					73:82	Fecal Microbiome	67:82	Fecal Microbiome	67:82	The Effects of Prebiotic Supplementation with OMNi-LOGiC® FIBRE on Fecal Microbiome, Fecal Volatile Organic Compounds, and Gut Permeability in Murine Neuroblastoma-Induced Tumor-Associated Cachexia.
32650568	0	86	from	Effects	4:10	arg1	Permeability					127:138	Gut Permeability	123:138	Gut Permeability	123:138	The Effects of Prebiotic Supplementation with OMNi-LOGiC® FIBRE on Fecal Microbiome, Fecal Volatile Organic Compounds, and Gut Permeability in Murine Neuroblastoma-Induced Tumor-Associated Cachexia.
32650568	0	86	from	Effects	4:10	arg1	Compounds					108:116	Fecal Volatile Organic Compounds	85:116	Fecal Volatile Organic Compounds	85:116	The Effects of Prebiotic Supplementation with OMNi-LOGiC® FIBRE on Fecal Microbiome, Fecal Volatile Organic Compounds, and Gut Permeability in Murine Neuroblastoma-Induced Tumor-Associated Cachexia.
32650568	0	87	theme	Volatile	91:98	arg1	Compounds					108:116	Fecal Volatile Organic Compounds	85:116	Fecal Volatile Organic Compounds	85:116	The Effects of Prebiotic Supplementation with OMNi-LOGiC® FIBRE on Fecal Microbiome, Fecal Volatile Organic Compounds, and Gut Permeability in Murine Neuroblastoma-Induced Tumor-Associated Cachexia.
32650568	6	88	theme	first	937:941	arg1	visibility					943:952	first visibility	937:952	first visibility to euthanasia	937:966	The median time of tumor growth (first visibility to euthanasia) was 37 days (IQR 12.5 days) in the NB-Aqua group and 37 days (IQR 36.5 days) in the NB-POS group (p = 0.791).
32650568	16	89	with	supplementation	2094:2108	arg1	FIBRE					2127:2131	FIBRE	2127:2131	FIBRE	2127:2131	Prebiotic supplementation with OMNi-LOGiC® FIBRE seemed to induce modifications of the fecal microbiome and VOC profile but did not improve gut permeability.
32650568	10	90	dep	Aqua	1558:1561	arg1	animals					1571:1577	animals	1571:1577	animals	1571:1577	Principal coordinate analysis (PCoA) revealed clustering differences between Aqua and POS animals.
32650568	3	91	theme	intestinal	495:504	arg1	microbiome					506:515	intestinal microbiome	495:515	intestinal microbiome	495:515	The aim of this project was to assess the effect of prebiotic supplementation with OMNi-LOGiC® FIBRE on intestinal microbiome, bacterial metabolism, gut permeability, and inflammation in a murine model of neuroblastoma (NB)-associated TAC.
32650568	3	92	from	effect	433:438	arg1	permeability					544:555	gut permeability	540:555	gut permeability	540:555	The aim of this project was to assess the effect of prebiotic supplementation with OMNi-LOGiC® FIBRE on intestinal microbiome, bacterial metabolism, gut permeability, and inflammation in a murine model of neuroblastoma (NB)-associated TAC.
32650568	3	92	from	effect	433:438	arg1	inflammation					562:573	inflammation	562:573	inflammation	562:573	The aim of this project was to assess the effect of prebiotic supplementation with OMNi-LOGiC® FIBRE on intestinal microbiome, bacterial metabolism, gut permeability, and inflammation in a murine model of neuroblastoma (NB)-associated TAC.
32650568	3	92	from	effect	433:438	arg1	microbiome					506:515	intestinal microbiome	495:515	intestinal microbiome	495:515	The aim of this project was to assess the effect of prebiotic supplementation with OMNi-LOGiC® FIBRE on intestinal microbiome, bacterial metabolism, gut permeability, and inflammation in a murine model of neuroblastoma (NB)-associated TAC.
32650568	3	92	from	effect	433:438	arg1	metabolism					528:537	bacterial metabolism	518:537	bacterial metabolism	518:537	The aim of this project was to assess the effect of prebiotic supplementation with OMNi-LOGiC® FIBRE on intestinal microbiome, bacterial metabolism, gut permeability, and inflammation in a murine model of neuroblastoma (NB)-associated TAC.
32650568	7	93	theme	fluorescein	1168:1178	arg1	permeability					1154:1165	gut permeability	1150:1165	gut permeability (fluorescein isothiocyanate-dextran (FITC-dextran)	1150:1216	At euthanasia, fecal microbiome and volatile organic compounds (VOCs), gut permeability (fluorescein isothiocyanate-dextran (FITC-dextran), and gut barrier markers were measured.
32650568	7	93	theme	fluorescein	1168:1178	arg1	isothiocyanate-dextran					1180:1201	fluorescein isothiocyanate-dextran	1168:1201	fluorescein isothiocyanate-dextran	1168:1201	At euthanasia, fecal microbiome and volatile organic compounds (VOCs), gut permeability (fluorescein isothiocyanate-dextran (FITC-dextran), and gut barrier markers were measured.
32650568	4	94	theme	200	758:760	arg1	mg					762:763	mg	762:763	mg	762:763	For this study, 2,000,000 NB cells (MHH-NB11) were implanted into athymic mice followed by daily supplementation with water or 200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC® FIBRE (NB-Aqua, n = 12; NB-POS, n = 12).
32650568	0	95	theme	Prebiotic	15:23	arg1	Supplementation					25:39	Prebiotic Supplementation	15:39	Prebiotic Supplementation with OMNi-LOGiC® FIBRE	15:62	The Effects of Prebiotic Supplementation with OMNi-LOGiC® FIBRE on Fecal Microbiome, Fecal Volatile Organic Compounds, and Gut Permeability in Murine Neuroblastoma-Induced Tumor-Associated Cachexia.
32650568	14	96	theme	junctions	1878:1886	arg1	leak					1855:1858	leak	1855:1858	leak	1855:1858	The pore and leak pathways of tight junctions did not differ between groups.
32650568	14	96	theme	junctions	1878:1886	arg1	pore					1846:1849	pore	1846:1849	pore	1846:1849	The pore and leak pathways of tight junctions did not differ between groups.
32650568	4	97	theme	prebiotic	765:773	arg1	OMNi-LOGiC®					797:807	200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC®	758:807	200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC®	758:807	For this study, 2,000,000 NB cells (MHH-NB11) were implanted into athymic mice followed by daily supplementation with water or 200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC® FIBRE (NB-Aqua, n = 12; NB-POS, n = 12).
32650568	12	98	theme	significant	1688:1698	arg1	differences					1700:1710	significant differences	1688:1710	significant differences between the groups	1688:1729	Of 49 VOCs, 22 showed significant differences between the groups.
32650568	1	99	theme	tumor-associated	228:243	arg1	TAC					255:257	TAC	255:257	TAC	255:257	Malignant diseases can cause tumor-associated cachexia (TAC).
32650568	1	99	theme	tumor-associated	228:243	arg1	cachexia					245:252	tumor-associated cachexia	228:252	tumor-associated cachexia (TAC)	228:258	Malignant diseases can cause tumor-associated cachexia (TAC).
32650568	10	100	theme	Principal	1481:1489	arg1	PCoA					1512:1515	PCoA	1512:1515	PCoA	1512:1515	Principal coordinate analysis (PCoA) revealed clustering differences between Aqua and POS animals.
32650568	10	100	theme	Principal	1481:1489	arg1	analysis					1502:1509	Principal coordinate analysis	1481:1509	Principal coordinate analysis (PCoA)	1481:1516	Principal coordinate analysis (PCoA) revealed clustering differences between Aqua and POS animals.
32650568	15	101	theme	VOC	2071:2073	arg1	profile					2075:2081	VOC profile	2071:2081	VOC profile	2071:2081	In conclusion, our results suggest that NB-induced TAC causes increased gut permeability coupled with compositional changes in the fecal microbiome and VOC profile.
32650568	9	102	theme	Alpha	1416:1420	arg1	diversity					1422:1430	Alpha diversity	1416:1430	Alpha diversity	1416:1430	Alpha diversity did not differ significantly between the groups.
32650568	3	103	theme	prebiotic	443:451	arg1	supplementation					453:467	prebiotic supplementation	443:467	prebiotic supplementation with OMNi-LOGiC® FIBRE	443:490	The aim of this project was to assess the effect of prebiotic supplementation with OMNi-LOGiC® FIBRE on intestinal microbiome, bacterial metabolism, gut permeability, and inflammation in a murine model of neuroblastoma (NB)-associated TAC.
32650568	3	104	theme	gut	540:542	arg1	permeability					544:555	gut permeability	540:555	gut permeability	540:555	The aim of this project was to assess the effect of prebiotic supplementation with OMNi-LOGiC® FIBRE on intestinal microbiome, bacterial metabolism, gut permeability, and inflammation in a murine model of neuroblastoma (NB)-associated TAC.
32650568	0	105	dep	Effects	4:10	arg1	Cachexia					189:196	Murine Neuroblastoma-Induced Tumor-Associated Cachexia	143:196	Murine Neuroblastoma-Induced Tumor-Associated Cachexia	143:196	The Effects of Prebiotic Supplementation with OMNi-LOGiC® FIBRE on Fecal Microbiome, Fecal Volatile Organic Compounds, and Gut Permeability in Murine Neuroblastoma-Induced Tumor-Associated Cachexia.
32650568	7	106	theme	volatile	1115:1122	arg1	compounds					1132:1140	volatile organic compounds	1115:1140	volatile organic compounds	1115:1140	At euthanasia, fecal microbiome and volatile organic compounds (VOCs), gut permeability (fluorescein isothiocyanate-dextran (FITC-dextran), and gut barrier markers were measured.
32650568	4	107	theme	NB	657:658	arg1	MHH-NB11					667:674	MHH-NB11	667:674	MHH-NB11	667:674	For this study, 2,000,000 NB cells (MHH-NB11) were implanted into athymic mice followed by daily supplementation with water or 200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC® FIBRE (NB-Aqua, n = 12; NB-POS, n = 12).
32650568	4	107	theme	NB	657:658	arg1	cells					660:664	2,000,000 NB cells	647:664	2,000,000 NB cells (MHH-NB11)	647:675	For this study, 2,000,000 NB cells (MHH-NB11) were implanted into athymic mice followed by daily supplementation with water or 200 mg prebiotic oligosaccharide (POS) OMNi-LOGiC® FIBRE (NB-Aqua, n = 12; NB-POS, n = 12).
34908474	4	0	theme	50 μg/kg	794:801	arg1	LPS					789:791	LPS	789:791	LPS (50 μg/kg body weight)	789:814	On day 36, six pigs were selected from each group, and LPS and LPS+CAT groups were administered with LPS (50 μg/kg body weight).
34908474	4	0	theme	50 μg/kg	794:801	arg1	weight					808:813	50 μg/kg body weight	794:813	50 μg/kg body weight	794:813	On day 36, six pigs were selected from each group, and LPS and LPS+CAT groups were administered with LPS (50 μg/kg body weight).
34908474	3	1	theme	2,000 mg/kg	643:653	arg1	supplementation					659:673	2,000 mg/kg CAT supplementation	643:673	2,000 mg/kg CAT supplementation	643:673	The pigs in CON and LPS groups were fed a basal diet, whereas the pigs in LPS+CAT group fed the basal diet with 2,000 mg/kg CAT supplementation for 35 days.
34908474	1	2	theme	catalase	296:303	arg1	effects					275:281	the protective effects	260:281	the protective effects of exogenous catalase (CAT) from microorganisms against lipopolysaccharide (LPS)-induced intestinal injury	260:388	The present study aimed to explore the protective effects of exogenous catalase (CAT) from microorganisms against lipopolysaccharide (LPS)-induced intestinal injury and its molecular mechanism in weaned pigs.
34908474	1	2	theme	catalase	296:303	arg1	mechanism					408:416	its molecular mechanism	394:416	its molecular mechanism in weaned pigs	394:431	The present study aimed to explore the protective effects of exogenous catalase (CAT) from microorganisms against lipopolysaccharide (LPS)-induced intestinal injury and its molecular mechanism in weaned pigs.
34908474	0	3	from	Microbiota	198:207	arg1	Pigs					219:222	Weaned Pigs	212:222	Weaned Pigs	212:222	Supplementation with Exogenous Catalase from Penicillium notatum in the Diet Ameliorates Lipopolysaccharide-Induced Intestinal Oxidative Damage through Affecting Intestinal Antioxidant Capacity and Microbiota in Weaned Pigs.
34908474	9	4	theme	weaned	1638:1643	arg1	pigs					1645:1648	weaned pigs	1638:1648	weaned pigs	1638:1648	Our study suggested that dietary supplemented with 2,000 mg/kg catalase was conducive to improve intestinal development and protect against LPS-induced intestinal mucosa injury via enhancing intestinal antioxidant capacity and altering microbiota composition in weaned pigs.
34908474	3	5	theme	CON	543:545	arg1	groups					555:560	CON and LPS groups	543:560	groups	555:560	The pigs in CON and LPS groups were fed a basal diet, whereas the pigs in LPS+CAT group fed the basal diet with 2,000 mg/kg CAT supplementation for 35 days.
34908474	5	6	theme	CON	828:830	arg1	Meanwhile					817:825	Meanwhile	817:825	Meanwhile	817:825	Meanwhile, CON group was injected with an equivalent amount of sterile saline.
34908474	5	6	theme	CON	828:830	arg1	group					832:836	CON group	828:836	CON group	828:836	Meanwhile, CON group was injected with an equivalent amount of sterile saline.
34908474	13	7	theme	intestinal	2266:2275	arg1	capacity					2289:2296	intestinal antioxidant capacity	2266:2296	intestinal antioxidant capacity	2266:2296	Our current study suggested that dietary supplemented with 2,000 mg/kg CAT was conducive to improve intestinal development and protect against LPS-induced intestinal mucosa injury via enhancing intestinal antioxidant capacity and altering microbiota composition in weaned pigs.
34908474	3	8	with	diet	633:636	arg1	supplementation					659:673	2,000 mg/kg CAT supplementation	643:673	2,000 mg/kg CAT supplementation	643:673	The pigs in CON and LPS groups were fed a basal diet, whereas the pigs in LPS+CAT group fed the basal diet with 2,000 mg/kg CAT supplementation for 35 days.
34908474	9	9	theme	intestinal	1473:1482	arg1	development					1484:1494	intestinal development	1473:1494	intestinal development	1473:1494	Our study suggested that dietary supplemented with 2,000 mg/kg catalase was conducive to improve intestinal development and protect against LPS-induced intestinal mucosa injury via enhancing intestinal antioxidant capacity and altering microbiota composition in weaned pigs.
34908474	11	10	theme	weaned	1886:1891	arg1	pigs					1893:1896	weaned pigs	1886:1896	weaned pigs	1886:1896	Recent study also found that exogenous CAT supplementation could improve growth performance and antioxidant capacity of weaned pigs.
34908474	1	11	theme	present	229:235	arg1	study					237:241	The present study	225:241	The present study	225:241	The present study aimed to explore the protective effects of exogenous catalase (CAT) from microorganisms against lipopolysaccharide (LPS)-induced intestinal injury and its molecular mechanism in weaned pigs.
34908474	8	12	theme	gut	1252:1254	arg1	dysbiosis					1267:1275	LPS-induced gut microbiota dysbiosis	1240:1275	LPS-induced gut microbiota dysbiosis	1240:1275	Besides, LPS-induced gut microbiota dysbiosis was significantly shifted by CAT through decreasing mainly Streptococcus and Escherichia-Shigella.
34908474	10	13	theme	Exogenous	1662:1670	arg1	CAT					1672:1674	IMPORTANCE Exogenous CAT	1651:1674	IMPORTANCE Exogenous CAT derived from microorganisms	1651:1702	IMPORTANCE Exogenous CAT derived from microorganisms has been widely used in food, medicine, and other industries.
34908474	1	14	theme	weaned	421:426	arg1	pigs					428:431	weaned pigs	421:431	weaned pigs	421:431	The present study aimed to explore the protective effects of exogenous catalase (CAT) from microorganisms against lipopolysaccharide (LPS)-induced intestinal injury and its molecular mechanism in weaned pigs.
34908474	3	15	theme	LPS	551:553	arg1	groups					555:560	CON and LPS groups	543:560	groups	555:560	The pigs in CON and LPS groups were fed a basal diet, whereas the pigs in LPS+CAT group fed the basal diet with 2,000 mg/kg CAT supplementation for 35 days.
34908474	12	16	theme	intestinal	2038:2047	arg1	damage					2049:2054	the oxidative stress-induced intestinal damage	2009:2054	the oxidative stress-induced intestinal damage in weaned pigs	2009:2069	However, it is still unknown that whether dietary exogenous CAT supplementation can provide a defense against the oxidative stress-induced intestinal damage in weaned pigs.
34908474	0	17	theme	Antioxidant	173:183	arg1	Capacity					185:192	Intestinal Antioxidant Capacity	162:192	Intestinal Antioxidant Capacity	162:192	Supplementation with Exogenous Catalase from Penicillium notatum in the Diet Ameliorates Lipopolysaccharide-Induced Intestinal Oxidative Damage through Affecting Intestinal Antioxidant Capacity and Microbiota in Weaned Pigs.
34908474	9	18	theme	intestinal	1528:1537	arg1	injury					1546:1551	LPS-induced intestinal mucosa injury	1516:1551	LPS-induced intestinal mucosa injury	1516:1551	Our study suggested that dietary supplemented with 2,000 mg/kg catalase was conducive to improve intestinal development and protect against LPS-induced intestinal mucosa injury via enhancing intestinal antioxidant capacity and altering microbiota composition in weaned pigs.
34908474	13	19	theme	LPS-induced	2215:2225	arg1	injury					2245:2250	LPS-induced intestinal mucosa injury	2215:2250	LPS-induced intestinal mucosa injury	2215:2250	Our current study suggested that dietary supplemented with 2,000 mg/kg CAT was conducive to improve intestinal development and protect against LPS-induced intestinal mucosa injury via enhancing intestinal antioxidant capacity and altering microbiota composition in weaned pigs.
34908474	6	20	theme	intestinal	943:952	arg1	mucosa					954:959	intestinal mucosa morphology and barrier	943:982	intestinal mucosa morphology and barrier	943:982	Results showed that LPS administration damaged intestinal mucosa morphology and barrier.
34908474	11	21	theme	CAT	1805:1807	arg1	supplementation					1809:1823	exogenous CAT supplementation	1795:1823	exogenous CAT supplementation	1795:1823	Recent study also found that exogenous CAT supplementation could improve growth performance and antioxidant capacity of weaned pigs.
34908474	7	22	theme	enterocyte	1209:1218	arg1	apoptosis					1220:1228	enterocyte apoptosis	1209:1228	enterocyte apoptosis	1209:1228	However, CAT supplementation alleviated the deleterious effects caused by LPS challenge through enhancing intestinal antioxidant capacity which was benefited to decrease proinflammatory cytokines concentrations and suppress enterocyte apoptosis.
34908474	1	23	theme	-induced	363:370	arg1	injury					383:388	lipopolysaccharide (LPS)-induced intestinal injury	339:388	lipopolysaccharide (LPS)-induced intestinal injury	339:388	The present study aimed to explore the protective effects of exogenous catalase (CAT) from microorganisms against lipopolysaccharide (LPS)-induced intestinal injury and its molecular mechanism in weaned pigs.
34908474	9	24	theme	antioxidant	1578:1588	arg1	capacity					1590:1597	intestinal antioxidant capacity	1567:1597	intestinal antioxidant capacity	1567:1597	Our study suggested that dietary supplemented with 2,000 mg/kg catalase was conducive to improve intestinal development and protect against LPS-induced intestinal mucosa injury via enhancing intestinal antioxidant capacity and altering microbiota composition in weaned pigs.
34908474	9	25	from	composition	1623:1633	arg1	pigs					1645:1648	weaned pigs	1638:1648	weaned pigs	1638:1648	Our study suggested that dietary supplemented with 2,000 mg/kg catalase was conducive to improve intestinal development and protect against LPS-induced intestinal mucosa injury via enhancing intestinal antioxidant capacity and altering microbiota composition in weaned pigs.
34908474	3	26	from	pigs	535:538	arg1	groups					555:560	CON and LPS groups	543:560	groups	555:560	The pigs in CON and LPS groups were fed a basal diet, whereas the pigs in LPS+CAT group fed the basal diet with 2,000 mg/kg CAT supplementation for 35 days.
34908474	0	27	from	Supplementation	0:14	arg1	Diet					72:75	the Diet	68:75	the Diet	68:75	Supplementation with Exogenous Catalase from Penicillium notatum in the Diet Ameliorates Lipopolysaccharide-Induced Intestinal Oxidative Damage through Affecting Intestinal Antioxidant Capacity and Microbiota in Weaned Pigs.
34908474	11	28	theme	growth	1839:1844	arg1	performance					1846:1856	growth performance	1839:1856	growth performance	1839:1856	Recent study also found that exogenous CAT supplementation could improve growth performance and antioxidant capacity of weaned pigs.
34908474	0	29	theme	Intestinal	116:125	arg1	Damage					137:142	Lipopolysaccharide-Induced Intestinal Oxidative Damage	89:142	Lipopolysaccharide-Induced Intestinal Oxidative Damage	89:142	Supplementation with Exogenous Catalase from Penicillium notatum in the Diet Ameliorates Lipopolysaccharide-Induced Intestinal Oxidative Damage through Affecting Intestinal Antioxidant Capacity and Microbiota in Weaned Pigs.
34908474	5	30	theme	sterile	880:886	arg1	saline					888:893	sterile saline	880:893	sterile saline	880:893	Meanwhile, CON group was injected with an equivalent amount of sterile saline.
34908474	2	31	theme	LPS+CAT	515:521	arg1	groups					523:528	LPS+CAT groups	515:528	LPS+CAT groups	515:528	Fifty-four weaned pigs (21 days of age) were randomly allocated to CON, LPS, and LPS+CAT groups.
34908474	0	32	from	notatum	57:63	arg1	Supplementation					0:14	Supplementation	0:14	Supplementation with Exogenous Catalase from Penicillium notatum in the Diet	0:75	Supplementation with Exogenous Catalase from Penicillium notatum in the Diet Ameliorates Lipopolysaccharide-Induced Intestinal Oxidative Damage through Affecting Intestinal Antioxidant Capacity and Microbiota in Weaned Pigs.
34908474	2	33	theme	Fifty-four	434:443	arg1	pigs					452:455	Fifty-four weaned pigs	434:455	Fifty-four weaned pigs (21 days of age)	434:472	Fifty-four weaned pigs (21 days of age) were randomly allocated to CON, LPS, and LPS+CAT groups.
34908474	3	34	from	pigs	597:600	arg1	group					613:617	LPS+CAT group	605:617	LPS+CAT group	605:617	The pigs in CON and LPS groups were fed a basal diet, whereas the pigs in LPS+CAT group fed the basal diet with 2,000 mg/kg CAT supplementation for 35 days.
34908474	7	35	theme	deleterious	1029:1039	arg1	effects					1041:1047	the deleterious effects	1025:1047	the deleterious effects caused by LPS challenge	1025:1071	However, CAT supplementation alleviated the deleterious effects caused by LPS challenge through enhancing intestinal antioxidant capacity which was benefited to decrease proinflammatory cytokines concentrations and suppress enterocyte apoptosis.
34908474	10	36	theme	other	1748:1752	arg1	industries					1754:1763	other industries	1748:1763	other industries	1748:1763	IMPORTANCE Exogenous CAT derived from microorganisms has been widely used in food, medicine, and other industries.
34908474	0	37	theme	Exogenous	21:29	arg1	Catalase					31:38	Exogenous Catalase	21:38	Exogenous Catalase	21:38	Supplementation with Exogenous Catalase from Penicillium notatum in the Diet Ameliorates Lipopolysaccharide-Induced Intestinal Oxidative Damage through Affecting Intestinal Antioxidant Capacity and Microbiota in Weaned Pigs.
34908474	2	38	dep	pigs	452:455	arg1	days					461:464	21 days	458:464	21 days of age	458:471	Fifty-four weaned pigs (21 days of age) were randomly allocated to CON, LPS, and LPS+CAT groups.
34908474	7	39	theme	antioxidant	1102:1112	arg1	capacity					1114:1121	intestinal antioxidant capacity	1091:1121	intestinal antioxidant capacity which was benefited to decrease proinflammatory cytokines concentrations and suppress enterocyte apoptosis	1091:1228	However, CAT supplementation alleviated the deleterious effects caused by LPS challenge through enhancing intestinal antioxidant capacity which was benefited to decrease proinflammatory cytokines concentrations and suppress enterocyte apoptosis.
34908474	13	40	theme	weaned	2337:2342	arg1	pigs					2344:2347	weaned pigs	2337:2347	weaned pigs	2337:2347	Our current study suggested that dietary supplemented with 2,000 mg/kg CAT was conducive to improve intestinal development and protect against LPS-induced intestinal mucosa injury via enhancing intestinal antioxidant capacity and altering microbiota composition in weaned pigs.
34908474	12	41	theme	exogenous	1949:1957	arg1	supplementation					1963:1977	dietary exogenous CAT supplementation	1941:1977	dietary exogenous CAT supplementation	1941:1977	However, it is still unknown that whether dietary exogenous CAT supplementation can provide a defense against the oxidative stress-induced intestinal damage in weaned pigs.
34908474	13	42	from	composition	2322:2332	arg1	pigs					2344:2347	weaned pigs	2337:2347	weaned pigs	2337:2347	Our current study suggested that dietary supplemented with 2,000 mg/kg CAT was conducive to improve intestinal development and protect against LPS-induced intestinal mucosa injury via enhancing intestinal antioxidant capacity and altering microbiota composition in weaned pigs.
34908474	3	43	theme	LPS+CAT	605:611	arg1	group					613:617	LPS+CAT group	605:617	LPS+CAT group	605:617	The pigs in CON and LPS groups were fed a basal diet, whereas the pigs in LPS+CAT group fed the basal diet with 2,000 mg/kg CAT supplementation for 35 days.
34908474	11	44	theme	exogenous	1795:1803	arg1	supplementation					1809:1823	exogenous CAT supplementation	1795:1823	exogenous CAT supplementation	1795:1823	Recent study also found that exogenous CAT supplementation could improve growth performance and antioxidant capacity of weaned pigs.
34908474	0	45	with	Supplementation	0:14	arg1	Catalase					31:38	Exogenous Catalase	21:38	Exogenous Catalase	21:38	Supplementation with Exogenous Catalase from Penicillium notatum in the Diet Ameliorates Lipopolysaccharide-Induced Intestinal Oxidative Damage through Affecting Intestinal Antioxidant Capacity and Microbiota in Weaned Pigs.
34908474	3	46	theme	basal	627:631	arg1	diet					633:636	the basal diet	623:636	the basal diet with 2,000 mg/kg CAT supplementation for 35 days	623:685	The pigs in CON and LPS groups were fed a basal diet, whereas the pigs in LPS+CAT group fed the basal diet with 2,000 mg/kg CAT supplementation for 35 days.
34908474	7	47	theme	LPS	1059:1061	arg1	challenge					1063:1071	LPS challenge	1059:1071	LPS challenge	1059:1071	However, CAT supplementation alleviated the deleterious effects caused by LPS challenge through enhancing intestinal antioxidant capacity which was benefited to decrease proinflammatory cytokines concentrations and suppress enterocyte apoptosis.
34908474	1	48	theme	exogenous	286:294	arg1	catalase					296:303	exogenous catalase	286:303	exogenous catalase (CAT) from microorganisms against lipopolysaccharide (LPS)-induced intestinal injury	286:388	The present study aimed to explore the protective effects of exogenous catalase (CAT) from microorganisms against lipopolysaccharide (LPS)-induced intestinal injury and its molecular mechanism in weaned pigs.
34908474	1	48	theme	exogenous	286:294	arg1	CAT					306:308	CAT	306:308	CAT	306:308	The present study aimed to explore the protective effects of exogenous catalase (CAT) from microorganisms against lipopolysaccharide (LPS)-induced intestinal injury and its molecular mechanism in weaned pigs.
34908474	13	49	theme	intestinal	2172:2181	arg1	development					2183:2193	intestinal development	2172:2193	intestinal development	2172:2193	Our current study suggested that dietary supplemented with 2,000 mg/kg CAT was conducive to improve intestinal development and protect against LPS-induced intestinal mucosa injury via enhancing intestinal antioxidant capacity and altering microbiota composition in weaned pigs.
34908474	10	50	theme	IMPORTANCE	1651:1660	arg1	CAT					1672:1674	IMPORTANCE Exogenous CAT	1651:1674	IMPORTANCE Exogenous CAT derived from microorganisms	1651:1702	IMPORTANCE Exogenous CAT derived from microorganisms has been widely used in food, medicine, and other industries.
34908474	3	51	theme	CAT	655:657	arg1	supplementation					659:673	2,000 mg/kg CAT supplementation	643:673	2,000 mg/kg CAT supplementation	643:673	The pigs in CON and LPS groups were fed a basal diet, whereas the pigs in LPS+CAT group fed the basal diet with 2,000 mg/kg CAT supplementation for 35 days.
34908474	4	52	theme	body	803:806	arg1	LPS					789:791	LPS	789:791	LPS (50 μg/kg body weight)	789:814	On day 36, six pigs were selected from each group, and LPS and LPS+CAT groups were administered with LPS (50 μg/kg body weight).
34908474	4	52	theme	body	803:806	arg1	weight					808:813	50 μg/kg body weight	794:813	50 μg/kg body weight	794:813	On day 36, six pigs were selected from each group, and LPS and LPS+CAT groups were administered with LPS (50 μg/kg body weight).
34908474	1	53	from	microorganisms	316:329	arg1	effects					275:281	the protective effects	260:281	the protective effects of exogenous catalase (CAT) from microorganisms against lipopolysaccharide (LPS)-induced intestinal injury	260:388	The present study aimed to explore the protective effects of exogenous catalase (CAT) from microorganisms against lipopolysaccharide (LPS)-induced intestinal injury and its molecular mechanism in weaned pigs.
34908474	1	53	from	microorganisms	316:329	arg1	catalase					296:303	exogenous catalase	286:303	exogenous catalase (CAT) from microorganisms against lipopolysaccharide (LPS)-induced intestinal injury	286:388	The present study aimed to explore the protective effects of exogenous catalase (CAT) from microorganisms against lipopolysaccharide (LPS)-induced intestinal injury and its molecular mechanism in weaned pigs.
34908474	1	53	from	microorganisms	316:329	arg1	mechanism					408:416	its molecular mechanism	394:416	its molecular mechanism in weaned pigs	394:431	The present study aimed to explore the protective effects of exogenous catalase (CAT) from microorganisms against lipopolysaccharide (LPS)-induced intestinal injury and its molecular mechanism in weaned pigs.
34908474	1	53	from	microorganisms	316:329	arg1	CAT					306:308	CAT	306:308	CAT	306:308	The present study aimed to explore the protective effects of exogenous catalase (CAT) from microorganisms against lipopolysaccharide (LPS)-induced intestinal injury and its molecular mechanism in weaned pigs.
34908474	3	54	dep	fed	567:569	arg1	whereas					585:591	whereas	585:591	whereas	585:591	The pigs in CON and LPS groups were fed a basal diet, whereas the pigs in LPS+CAT group fed the basal diet with 2,000 mg/kg CAT supplementation for 35 days.
34908474	12	55	theme	weaned	2059:2064	arg1	pigs					2066:2069	weaned pigs	2059:2069	weaned pigs	2059:2069	However, it is still unknown that whether dietary exogenous CAT supplementation can provide a defense against the oxidative stress-induced intestinal damage in weaned pigs.
34908474	11	56	theme	pigs	1893:1896	arg1	performance					1846:1856	growth performance	1839:1856	growth performance	1839:1856	Recent study also found that exogenous CAT supplementation could improve growth performance and antioxidant capacity of weaned pigs.
34908474	11	56	theme	pigs	1893:1896	arg1	capacity					1874:1881	antioxidant capacity	1862:1881	antioxidant capacity	1862:1881	Recent study also found that exogenous CAT supplementation could improve growth performance and antioxidant capacity of weaned pigs.
34908474	0	57	theme	Intestinal	162:171	arg1	Capacity					185:192	Intestinal Antioxidant Capacity	162:192	Intestinal Antioxidant Capacity	162:192	Supplementation with Exogenous Catalase from Penicillium notatum in the Diet Ameliorates Lipopolysaccharide-Induced Intestinal Oxidative Damage through Affecting Intestinal Antioxidant Capacity and Microbiota in Weaned Pigs.
34908474	8	58	theme	LPS-induced	1240:1250	arg1	dysbiosis					1267:1275	LPS-induced gut microbiota dysbiosis	1240:1275	LPS-induced gut microbiota dysbiosis	1240:1275	Besides, LPS-induced gut microbiota dysbiosis was significantly shifted by CAT through decreasing mainly Streptococcus and Escherichia-Shigella.
34908474	3	59	theme	basal	573:577	arg1	diet					579:582	a basal diet	571:582	a basal diet	571:582	The pigs in CON and LPS groups were fed a basal diet, whereas the pigs in LPS+CAT group fed the basal diet with 2,000 mg/kg CAT supplementation for 35 days.
34908474	9	60	theme	LPS-induced	1516:1526	arg1	injury					1546:1551	LPS-induced intestinal mucosa injury	1516:1551	LPS-induced intestinal mucosa injury	1516:1551	Our study suggested that dietary supplemented with 2,000 mg/kg catalase was conducive to improve intestinal development and protect against LPS-induced intestinal mucosa injury via enhancing intestinal antioxidant capacity and altering microbiota composition in weaned pigs.
34908474	12	61	theme	stress-induced	2023:2036	arg1	damage					2049:2054	the oxidative stress-induced intestinal damage	2009:2054	the oxidative stress-induced intestinal damage in weaned pigs	2009:2069	However, it is still unknown that whether dietary exogenous CAT supplementation can provide a defense against the oxidative stress-induced intestinal damage in weaned pigs.
34908474	8	62	theme	microbiota	1256:1265	arg1	dysbiosis					1267:1275	LPS-induced gut microbiota dysbiosis	1240:1275	LPS-induced gut microbiota dysbiosis	1240:1275	Besides, LPS-induced gut microbiota dysbiosis was significantly shifted by CAT through decreasing mainly Streptococcus and Escherichia-Shigella.
34908474	7	63	theme	proinflammatory	1155:1169	arg1	cytokines					1171:1179	proinflammatory cytokines	1155:1179	proinflammatory cytokines concentrations	1155:1194	However, CAT supplementation alleviated the deleterious effects caused by LPS challenge through enhancing intestinal antioxidant capacity which was benefited to decrease proinflammatory cytokines concentrations and suppress enterocyte apoptosis.
34908474	1	64	from	effects	275:281	arg1	microorganisms					316:329	microorganisms	316:329	microorganisms against lipopolysaccharide (LPS)-induced intestinal injury	316:388	The present study aimed to explore the protective effects of exogenous catalase (CAT) from microorganisms against lipopolysaccharide (LPS)-induced intestinal injury and its molecular mechanism in weaned pigs.
34908474	1	64	from	effects	275:281	arg1	pigs					428:431	weaned pigs	421:431	weaned pigs	421:431	The present study aimed to explore the protective effects of exogenous catalase (CAT) from microorganisms against lipopolysaccharide (LPS)-induced intestinal injury and its molecular mechanism in weaned pigs.
34908474	9	65	theme	mucosa	1539:1544	arg1	injury					1546:1551	LPS-induced intestinal mucosa injury	1516:1551	LPS-induced intestinal mucosa injury	1516:1551	Our study suggested that dietary supplemented with 2,000 mg/kg catalase was conducive to improve intestinal development and protect against LPS-induced intestinal mucosa injury via enhancing intestinal antioxidant capacity and altering microbiota composition in weaned pigs.
34908474	13	66	theme	antioxidant	2277:2287	arg1	capacity					2289:2296	intestinal antioxidant capacity	2266:2296	intestinal antioxidant capacity	2266:2296	Our current study suggested that dietary supplemented with 2,000 mg/kg CAT was conducive to improve intestinal development and protect against LPS-induced intestinal mucosa injury via enhancing intestinal antioxidant capacity and altering microbiota composition in weaned pigs.
34908474	7	67	theme	CAT	994:996	arg1	supplementation					998:1012	CAT supplementation	994:1012	CAT supplementation	994:1012	However, CAT supplementation alleviated the deleterious effects caused by LPS challenge through enhancing intestinal antioxidant capacity which was benefited to decrease proinflammatory cytokines concentrations and suppress enterocyte apoptosis.
34908474	0	68	theme	Weaned	212:217	arg1	Pigs					219:222	Weaned Pigs	212:222	Weaned Pigs	212:222	Supplementation with Exogenous Catalase from Penicillium notatum in the Diet Ameliorates Lipopolysaccharide-Induced Intestinal Oxidative Damage through Affecting Intestinal Antioxidant Capacity and Microbiota in Weaned Pigs.
34908474	10	69	attach	derived	1676:1682	arg1	microorganisms					1689:1702	microorganisms	1689:1702	microorganisms	1689:1702	IMPORTANCE Exogenous CAT derived from microorganisms has been widely used in food, medicine, and other industries.
34908474	10	69	attach	derived	1676:1682	arg2	CAT					1672:1674	IMPORTANCE Exogenous CAT	1651:1674	IMPORTANCE Exogenous CAT derived from microorganisms	1651:1702	IMPORTANCE Exogenous CAT derived from microorganisms has been widely used in food, medicine, and other industries.
34908474	13	70	theme	2,000 mg/kg	2131:2141	arg1	CAT					2143:2145	2,000 mg/kg CAT	2131:2145	2,000 mg/kg CAT	2131:2145	Our current study suggested that dietary supplemented with 2,000 mg/kg CAT was conducive to improve intestinal development and protect against LPS-induced intestinal mucosa injury via enhancing intestinal antioxidant capacity and altering microbiota composition in weaned pigs.
34908474	14	71	theme	stress-induced	2467:2480	arg1	injury					2482:2487	various oxidative stress-induced injury	2449:2487	various oxidative stress-induced injury	2449:2487	Moreover, this study will also assist in developing of CAT produced by microorganisms to attenuate various oxidative stress-induced injury or diseases.
34908474	13	72	theme	intestinal	2227:2236	arg1	injury					2245:2250	LPS-induced intestinal mucosa injury	2215:2250	LPS-induced intestinal mucosa injury	2215:2250	Our current study suggested that dietary supplemented with 2,000 mg/kg CAT was conducive to improve intestinal development and protect against LPS-induced intestinal mucosa injury via enhancing intestinal antioxidant capacity and altering microbiota composition in weaned pigs.
34908474	9	73	theme	intestinal	1567:1576	arg1	capacity					1590:1597	intestinal antioxidant capacity	1567:1597	intestinal antioxidant capacity	1567:1597	Our study suggested that dietary supplemented with 2,000 mg/kg catalase was conducive to improve intestinal development and protect against LPS-induced intestinal mucosa injury via enhancing intestinal antioxidant capacity and altering microbiota composition in weaned pigs.
34908474	0	74	theme	Lipopolysaccharide-Induced	89:114	arg1	Damage					137:142	Lipopolysaccharide-Induced Intestinal Oxidative Damage	89:142	Lipopolysaccharide-Induced Intestinal Oxidative Damage	89:142	Supplementation with Exogenous Catalase from Penicillium notatum in the Diet Ameliorates Lipopolysaccharide-Induced Intestinal Oxidative Damage through Affecting Intestinal Antioxidant Capacity and Microbiota in Weaned Pigs.
34908474	1	75	theme	intestinal	372:381	arg1	injury					383:388	lipopolysaccharide (LPS)-induced intestinal injury	339:388	lipopolysaccharide (LPS)-induced intestinal injury	339:388	The present study aimed to explore the protective effects of exogenous catalase (CAT) from microorganisms against lipopolysaccharide (LPS)-induced intestinal injury and its molecular mechanism in weaned pigs.
34908474	6	76	theme	LPS	916:918	arg1	administration					920:933	LPS administration	916:933	LPS administration	916:933	Results showed that LPS administration damaged intestinal mucosa morphology and barrier.
34908474	11	77	theme	antioxidant	1862:1872	arg1	capacity					1874:1881	antioxidant capacity	1862:1881	antioxidant capacity	1862:1881	Recent study also found that exogenous CAT supplementation could improve growth performance and antioxidant capacity of weaned pigs.
34908474	0	78	theme	Oxidative	127:135	arg1	Damage					137:142	Lipopolysaccharide-Induced Intestinal Oxidative Damage	89:142	Lipopolysaccharide-Induced Intestinal Oxidative Damage	89:142	Supplementation with Exogenous Catalase from Penicillium notatum in the Diet Ameliorates Lipopolysaccharide-Induced Intestinal Oxidative Damage through Affecting Intestinal Antioxidant Capacity and Microbiota in Weaned Pigs.
34908474	12	79	theme	oxidative	2013:2021	arg1	damage					2049:2054	the oxidative stress-induced intestinal damage	2009:2054	the oxidative stress-induced intestinal damage in weaned pigs	2009:2069	However, it is still unknown that whether dietary exogenous CAT supplementation can provide a defense against the oxidative stress-induced intestinal damage in weaned pigs.
34908474	9	80	theme	microbiota	1612:1621	arg1	composition					1623:1633	microbiota composition	1612:1633	microbiota composition in weaned pigs	1612:1648	Our study suggested that dietary supplemented with 2,000 mg/kg catalase was conducive to improve intestinal development and protect against LPS-induced intestinal mucosa injury via enhancing intestinal antioxidant capacity and altering microbiota composition in weaned pigs.
34908474	1	81	theme	molecular	398:406	arg1	mechanism					408:416	its molecular mechanism	394:416	its molecular mechanism in weaned pigs	394:431	The present study aimed to explore the protective effects of exogenous catalase (CAT) from microorganisms against lipopolysaccharide (LPS)-induced intestinal injury and its molecular mechanism in weaned pigs.
34908474	5	82	theme	saline	888:893	arg1	amount					870:875	an equivalent amount	856:875	an equivalent amount of sterile saline	856:893	Meanwhile, CON group was injected with an equivalent amount of sterile saline.
34908474	5	82	theme	saline	888:893	arg1	saline					888:893	sterile saline	880:893	sterile saline	880:893	Meanwhile, CON group was injected with an equivalent amount of sterile saline.
34908474	6	83	dep	mucosa	954:959	arg1	barrier					976:982	barrier	976:982	barrier	976:982	Results showed that LPS administration damaged intestinal mucosa morphology and barrier.
34908474	6	83	dep	mucosa	954:959	arg1	morphology					961:970	morphology	961:970	morphology	961:970	Results showed that LPS administration damaged intestinal mucosa morphology and barrier.
34908474	13	84	theme	microbiota	2311:2320	arg1	composition					2322:2332	microbiota composition	2311:2332	microbiota composition in weaned pigs	2311:2347	Our current study suggested that dietary supplemented with 2,000 mg/kg CAT was conducive to improve intestinal development and protect against LPS-induced intestinal mucosa injury via enhancing intestinal antioxidant capacity and altering microbiota composition in weaned pigs.
34908474	1	85	from	mechanism	408:416	arg1	microorganisms					316:329	microorganisms	316:329	microorganisms against lipopolysaccharide (LPS)-induced intestinal injury	316:388	The present study aimed to explore the protective effects of exogenous catalase (CAT) from microorganisms against lipopolysaccharide (LPS)-induced intestinal injury and its molecular mechanism in weaned pigs.
34908474	1	85	from	mechanism	408:416	arg1	pigs					428:431	weaned pigs	421:431	weaned pigs	421:431	The present study aimed to explore the protective effects of exogenous catalase (CAT) from microorganisms against lipopolysaccharide (LPS)-induced intestinal injury and its molecular mechanism in weaned pigs.
34908474	10	86	used	used	1720:1723	arg2	CAT					1672:1674	IMPORTANCE Exogenous CAT	1651:1674	IMPORTANCE Exogenous CAT derived from microorganisms	1651:1702	IMPORTANCE Exogenous CAT derived from microorganisms has been widely used in food, medicine, and other industries.
34908474	2	87	theme	age	469:471	arg1	days					461:464	21 days	458:464	21 days of age	458:471	Fifty-four weaned pigs (21 days of age) were randomly allocated to CON, LPS, and LPS+CAT groups.
34908474	12	88	theme	dietary	1941:1947	arg1	supplementation					1963:1977	dietary exogenous CAT supplementation	1941:1977	dietary exogenous CAT supplementation	1941:1977	However, it is still unknown that whether dietary exogenous CAT supplementation can provide a defense against the oxidative stress-induced intestinal damage in weaned pigs.
34908474	5	89	theme	equivalent	859:868	arg1	amount					870:875	an equivalent amount	856:875	an equivalent amount of sterile saline	856:893	Meanwhile, CON group was injected with an equivalent amount of sterile saline.
34908474	5	89	theme	equivalent	859:868	arg1	saline					888:893	sterile saline	880:893	sterile saline	880:893	Meanwhile, CON group was injected with an equivalent amount of sterile saline.
34908474	11	90	theme	Recent	1766:1771	arg1	study					1773:1777	Recent study	1766:1777	Recent study	1766:1777	Recent study also found that exogenous CAT supplementation could improve growth performance and antioxidant capacity of weaned pigs.
34908474	7	91	theme	intestinal	1091:1100	arg1	capacity					1114:1121	intestinal antioxidant capacity	1091:1121	intestinal antioxidant capacity which was benefited to decrease proinflammatory cytokines concentrations and suppress enterocyte apoptosis	1091:1228	However, CAT supplementation alleviated the deleterious effects caused by LPS challenge through enhancing intestinal antioxidant capacity which was benefited to decrease proinflammatory cytokines concentrations and suppress enterocyte apoptosis.
34908474	12	92	theme	CAT	1959:1961	arg1	supplementation					1963:1977	dietary exogenous CAT supplementation	1941:1977	dietary exogenous CAT supplementation	1941:1977	However, it is still unknown that whether dietary exogenous CAT supplementation can provide a defense against the oxidative stress-induced intestinal damage in weaned pigs.
34908474	4	93	theme	LPS	743:745	arg1	groups					759:764	LPS and LPS+CAT groups	743:764	LPS and LPS+CAT groups	743:764	On day 36, six pigs were selected from each group, and LPS and LPS+CAT groups were administered with LPS (50 μg/kg body weight).
34908474	2	94	theme	weaned	445:450	arg1	pigs					452:455	Fifty-four weaned pigs	434:455	Fifty-four weaned pigs (21 days of age)	434:472	Fifty-four weaned pigs (21 days of age) were randomly allocated to CON, LPS, and LPS+CAT groups.
34908474	4	95	theme	LPS+CAT	751:757	arg1	groups					759:764	LPS and LPS+CAT groups	743:764	LPS and LPS+CAT groups	743:764	On day 36, six pigs were selected from each group, and LPS and LPS+CAT groups were administered with LPS (50 μg/kg body weight).
34908474	13	96	theme	mucosa	2238:2243	arg1	injury					2245:2250	LPS-induced intestinal mucosa injury	2215:2250	LPS-induced intestinal mucosa injury	2215:2250	Our current study suggested that dietary supplemented with 2,000 mg/kg CAT was conducive to improve intestinal development and protect against LPS-induced intestinal mucosa injury via enhancing intestinal antioxidant capacity and altering microbiota composition in weaned pigs.
34908474	12	97	from	damage	2049:2054	arg1	pigs					2066:2069	weaned pigs	2059:2069	weaned pigs	2059:2069	However, it is still unknown that whether dietary exogenous CAT supplementation can provide a defense against the oxidative stress-induced intestinal damage in weaned pigs.
34908474	0	98	from	Capacity	185:192	arg1	Pigs					219:222	Weaned Pigs	212:222	Weaned Pigs	212:222	Supplementation with Exogenous Catalase from Penicillium notatum in the Diet Ameliorates Lipopolysaccharide-Induced Intestinal Oxidative Damage through Affecting Intestinal Antioxidant Capacity and Microbiota in Weaned Pigs.
34908474	7	99	dep	cytokines	1171:1179	arg1	concentrations					1181:1194	concentrations	1181:1194	proinflammatory cytokines concentrations	1155:1194	However, CAT supplementation alleviated the deleterious effects caused by LPS challenge through enhancing intestinal antioxidant capacity which was benefited to decrease proinflammatory cytokines concentrations and suppress enterocyte apoptosis.
34908474	13	100	theme	current	2076:2082	arg1	study					2084:2088	Our current study	2072:2088	Our current study	2072:2088	Our current study suggested that dietary supplemented with 2,000 mg/kg CAT was conducive to improve intestinal development and protect against LPS-induced intestinal mucosa injury via enhancing intestinal antioxidant capacity and altering microbiota composition in weaned pigs.
34908474	1	101	theme	protective	264:273	arg1	effects					275:281	the protective effects	260:281	the protective effects of exogenous catalase (CAT) from microorganisms against lipopolysaccharide (LPS)-induced intestinal injury	260:388	The present study aimed to explore the protective effects of exogenous catalase (CAT) from microorganisms against lipopolysaccharide (LPS)-induced intestinal injury and its molecular mechanism in weaned pigs.
34908474	9	102	theme	2,000 mg/kg	1427:1437	arg1	catalase					1439:1446	2,000 mg/kg catalase	1427:1446	2,000 mg/kg catalase	1427:1446	Our study suggested that dietary supplemented with 2,000 mg/kg catalase was conducive to improve intestinal development and protect against LPS-induced intestinal mucosa injury via enhancing intestinal antioxidant capacity and altering microbiota composition in weaned pigs.
34199466	10	0	theme	short-chain	1600:1610	arg1	acids					1618:1622	short-chain fatty acids	1600:1622	short-chain fatty acids (SCFAs)	1600:1630	Furthermore, we observed that, at the operational taxonomic unit (OTU) level, Cyanobacteria were depleted, while short-chain fatty acids (SCFAs), such as isobutyric acid, acetic acid, and propionic acid, were increased in saffron-treated mice.
34199466	10	0	theme	short-chain	1600:1610	arg1	acid					1652:1655	isobutyric acid	1641:1655	isobutyric acid	1641:1655	Furthermore, we observed that, at the operational taxonomic unit (OTU) level, Cyanobacteria were depleted, while short-chain fatty acids (SCFAs), such as isobutyric acid, acetic acid, and propionic acid, were increased in saffron-treated mice.
34199466	10	0	theme	short-chain	1600:1610	arg1	acid					1685:1688	propionic acid	1675:1688	propionic acid	1675:1688	Furthermore, we observed that, at the operational taxonomic unit (OTU) level, Cyanobacteria were depleted, while short-chain fatty acids (SCFAs), such as isobutyric acid, acetic acid, and propionic acid, were increased in saffron-treated mice.
34199466	10	0	theme	short-chain	1600:1610	arg1	SCFAs					1625:1629	SCFAs	1625:1629	SCFAs	1625:1629	Furthermore, we observed that, at the operational taxonomic unit (OTU) level, Cyanobacteria were depleted, while short-chain fatty acids (SCFAs), such as isobutyric acid, acetic acid, and propionic acid, were increased in saffron-treated mice.
34199466	10	0	theme	short-chain	1600:1610	arg1	acid					1665:1668	acetic acid	1658:1668	acetic acid	1658:1668	Furthermore, we observed that, at the operational taxonomic unit (OTU) level, Cyanobacteria were depleted, while short-chain fatty acids (SCFAs), such as isobutyric acid, acetic acid, and propionic acid, were increased in saffron-treated mice.
34199466	6	1	theme	colonic	889:895	arg1	mucosa					897:902	the colonic mucosa	885:902	the colonic mucosa	885:902	Saffron pre-treatment improved the gross and histopathological characteristics of the colonic mucosa in murine experimental colitis.
34199466	8	2	theme	NF-κB	1247:1251	arg1	translocation					1230:1242	the nuclear translocation	1218:1242	the nuclear translocation of NF-κB	1218:1251	Saffron treatment significantly decreased secretion of serotonin and pro-inflammatory cytokines, such as TNF-α, IL-1β, and IL-6, in the colon tissues by suppressing the nuclear translocation of NF-κB.
34199466	9	3	theme	distinct	1291:1298	arg1	clusters					1300:1307	distinct clusters	1291:1307	distinct clusters in the saffron-treated and untreated mice	1291:1349	The gut microbiome analysis revealed distinct clusters in the saffron-treated and untreated mice in dextran sulfate sodium (DSS)-induced colitis by visualization of the Bray-Curtis diversity by principal coordinates analysis (PCoA).
34199466	1	4	theme	chronic	175:181	arg1	condition					196:204	a chronic inflammatory condition	173:204	a chronic inflammatory condition of the gastrointestinal tract with an incompletely understood pathogenesis	173:279	Inflammatory bowel disease (IBD) is a chronic inflammatory condition of the gastrointestinal tract with an incompletely understood pathogenesis.
34199466	1	4	theme	chronic	175:181	arg1	disease					156:162	Inflammatory bowel disease	137:162	Inflammatory bowel disease (IBD)	137:168	Inflammatory bowel disease (IBD) is a chronic inflammatory condition of the gastrointestinal tract with an incompletely understood pathogenesis.
34199466	6	5	theme	gross	838:842	arg1	characteristics					866:880	the gross and histopathological characteristics	834:880	the gross and histopathological characteristics of the colonic mucosa in murine experimental colitis	834:933	Saffron pre-treatment improved the gross and histopathological characteristics of the colonic mucosa in murine experimental colitis.
34199466	2	6	theme	colon	341:345	arg1	cancer					347:352	colon cancer	341:352	colon cancer	341:352	Long-standing colitis is associated with increased risk of colon cancer.
34199466	9	7	theme	gut	1258:1260	arg1	analysis					1273:1280	The gut microbiome analysis	1254:1280	The gut microbiome analysis	1254:1280	The gut microbiome analysis revealed distinct clusters in the saffron-treated and untreated mice in dextran sulfate sodium (DSS)-induced colitis by visualization of the Bray-Curtis diversity by principal coordinates analysis (PCoA).
34199466	11	8	theme	microbiota	1852:1861	arg1	composition					1863:1873	gut microbiota composition	1848:1873	gut microbiota composition	1848:1873	Our data suggest that pre-treatment with saffron inhibits DSS-induced pro-inflammatory cytokine secretion, modulates gut microbiota composition, prevents the depletion of SCFAs, and reduces the susceptibility to colitis.
34199466	5	9	theme	prophylactic	756:767	arg1	effect					769:774	a prophylactic effect	754:774	a prophylactic effect	754:774	In our study, we investigated whether saffron may have a prophylactic effect in a murine colitis model.
34199466	9	10	theme	saffron-treated	1316:1330	arg1	mice					1346:1349	the saffron-treated and untreated mice	1312:1349	the saffron-treated and untreated mice	1312:1349	The gut microbiome analysis revealed distinct clusters in the saffron-treated and untreated mice in dextran sulfate sodium (DSS)-induced colitis by visualization of the Bray-Curtis diversity by principal coordinates analysis (PCoA).
34199466	10	11	theme	propionic	1675:1683	arg1	acid					1685:1688	propionic acid	1675:1688	propionic acid	1675:1688	Furthermore, we observed that, at the operational taxonomic unit (OTU) level, Cyanobacteria were depleted, while short-chain fatty acids (SCFAs), such as isobutyric acid, acetic acid, and propionic acid, were increased in saffron-treated mice.
34199466	9	12	theme	untreated	1336:1344	arg1	mice					1346:1349	the saffron-treated and untreated mice	1312:1349	the saffron-treated and untreated mice	1312:1349	The gut microbiome analysis revealed distinct clusters in the saffron-treated and untreated mice in dextran sulfate sodium (DSS)-induced colitis by visualization of the Bray-Curtis diversity by principal coordinates analysis (PCoA).
34199466	1	13	theme	tract	230:234	arg1	condition					196:204	a chronic inflammatory condition	173:204	a chronic inflammatory condition of the gastrointestinal tract with an incompletely understood pathogenesis	173:279	Inflammatory bowel disease (IBD) is a chronic inflammatory condition of the gastrointestinal tract with an incompletely understood pathogenesis.
34199466	1	13	theme	tract	230:234	arg1	disease					156:162	Inflammatory bowel disease	137:162	Inflammatory bowel disease (IBD)	137:168	Inflammatory bowel disease (IBD) is a chronic inflammatory condition of the gastrointestinal tract with an incompletely understood pathogenesis.
34199466	10	14	theme	acetic	1658:1663	arg1	acid					1665:1668	acetic acid	1658:1668	acetic acid	1658:1668	Furthermore, we observed that, at the operational taxonomic unit (OTU) level, Cyanobacteria were depleted, while short-chain fatty acids (SCFAs), such as isobutyric acid, acetic acid, and propionic acid, were increased in saffron-treated mice.
34199466	1	15	theme	Inflammatory	137:148	arg1	IBD					165:167	IBD	165:167	IBD	165:167	Inflammatory bowel disease (IBD) is a chronic inflammatory condition of the gastrointestinal tract with an incompletely understood pathogenesis.
34199466	1	15	theme	Inflammatory	137:148	arg1	disease					156:162	Inflammatory bowel disease	137:162	Inflammatory bowel disease (IBD)	137:168	Inflammatory bowel disease (IBD) is a chronic inflammatory condition of the gastrointestinal tract with an incompletely understood pathogenesis.
34199466	1	15	theme	Inflammatory	137:148	arg1	condition					196:204	a chronic inflammatory condition	173:204	a chronic inflammatory condition of the gastrointestinal tract with an incompletely understood pathogenesis	173:279	Inflammatory bowel disease (IBD) is a chronic inflammatory condition of the gastrointestinal tract with an incompletely understood pathogenesis.
34199466	11	16	theme	pro-inflammatory	1801:1816	arg1	secretion					1827:1835	DSS-induced pro-inflammatory cytokine secretion	1789:1835	DSS-induced pro-inflammatory cytokine secretion	1789:1835	Our data suggest that pre-treatment with saffron inhibits DSS-induced pro-inflammatory cytokine secretion, modulates gut microbiota composition, prevents the depletion of SCFAs, and reduces the susceptibility to colitis.
34199466	2	17	theme	Long-standing	282:294	arg1	colitis					296:302	Long-standing colitis	282:302	Long-standing colitis	282:302	Long-standing colitis is associated with increased risk of colon cancer.
34199466	10	18	theme	operational	1525:1535	arg1	level					1558:1562	the operational taxonomic unit (OTU) level	1521:1562	the operational taxonomic unit (OTU) level	1521:1562	Furthermore, we observed that, at the operational taxonomic unit (OTU) level, Cyanobacteria were depleted, while short-chain fatty acids (SCFAs), such as isobutyric acid, acetic acid, and propionic acid, were increased in saffron-treated mice.
34199466	0	19	theme	Microbiome	84:93	arg1	Composition					95:105	Microbiome Composition	84:105	Microbiome Composition in Experimental Colitis Mice	84:134	Saffron Pre-Treatment Promotes Reduction in Tissue Inflammatory Profiles and Alters Microbiome Composition in Experimental Colitis Mice.
34199466	8	20	theme	cytokines	1139:1147	arg1	secretion					1095:1103	secretion	1095:1103	secretion of serotonin and pro-inflammatory cytokines, such as TNF-α, IL-1β, and IL-6, in the colon tissues	1095:1201	Saffron treatment significantly decreased secretion of serotonin and pro-inflammatory cytokines, such as TNF-α, IL-1β, and IL-6, in the colon tissues by suppressing the nuclear translocation of NF-κB.
34199466	3	21	theme	anti-inflammatory	391:407	arg1	drugs					430:434	various anti-inflammatory and immunomodulatory drugs	383:434	various anti-inflammatory and immunomodulatory drugs	383:434	Despite the availability of various anti-inflammatory and immunomodulatory drugs, many patients fail to respond to pharmacologic therapy and some experience drug-induced adverse events.
34199466	5	22	theme	murine	781:786	arg1	model					796:800	a murine colitis model	779:800	a murine colitis model	779:800	In our study, we investigated whether saffron may have a prophylactic effect in a murine colitis model.
34199466	7	23	theme	mice	1041:1044	arg1	group					1046:1050	the vehicle-treated mice group	1021:1050	the vehicle-treated mice group	1021:1050	Treatment with saffron showed a significant amelioration of colitis when compared to the vehicle-treated mice group.
34199466	10	24	theme	OTU	1553:1555	arg1	level					1558:1562	the operational taxonomic unit (OTU) level	1521:1562	the operational taxonomic unit (OTU) level	1521:1562	Furthermore, we observed that, at the operational taxonomic unit (OTU) level, Cyanobacteria were depleted, while short-chain fatty acids (SCFAs), such as isobutyric acid, acetic acid, and propionic acid, were increased in saffron-treated mice.
34199466	3	25	theme	adverse	525:531	arg1	events					533:538	drug-induced adverse events	512:538	drug-induced adverse events	512:538	Despite the availability of various anti-inflammatory and immunomodulatory drugs, many patients fail to respond to pharmacologic therapy and some experience drug-induced adverse events.
34199466	0	26	theme	Colitis	123:129	arg1	Mice					131:134	Experimental Colitis Mice	110:134	Experimental Colitis Mice	110:134	Saffron Pre-Treatment Promotes Reduction in Tissue Inflammatory Profiles and Alters Microbiome Composition in Experimental Colitis Mice.
34199466	6	27	from	characteristics	866:880	arg1	colitis					927:933	murine experimental colitis	907:933	murine experimental colitis	907:933	Saffron pre-treatment improved the gross and histopathological characteristics of the colonic mucosa in murine experimental colitis.
34199466	4	28	theme	Dietary	541:547	arg1	supplements					549:559	Dietary supplements	541:559	Dietary supplements	541:559	Dietary supplements, particularly saffron (Crocus sativus), have recently gained an appreciable attention in alleviating some symptoms of digestive diseases.
34199466	4	28	theme	Dietary	541:547	arg1	saffron					575:581	saffron	575:581	particularly saffron (Crocus sativus)	562:598	Dietary supplements, particularly saffron (Crocus sativus), have recently gained an appreciable attention in alleviating some symptoms of digestive diseases.
34199466	10	29	theme	unit	1547:1550	arg1	level					1558:1562	the operational taxonomic unit (OTU) level	1521:1562	the operational taxonomic unit (OTU) level	1521:1562	Furthermore, we observed that, at the operational taxonomic unit (OTU) level, Cyanobacteria were depleted, while short-chain fatty acids (SCFAs), such as isobutyric acid, acetic acid, and propionic acid, were increased in saffron-treated mice.
34199466	0	30	theme	Saffron	0:6	arg1	Pre-Treatment					8:20	Saffron Pre-Treatment	0:20	Saffron Pre-Treatment	0:20	Saffron Pre-Treatment Promotes Reduction in Tissue Inflammatory Profiles and Alters Microbiome Composition in Experimental Colitis Mice.
34199466	3	31	theme	immunomodulatory	413:428	arg1	drugs					430:434	various anti-inflammatory and immunomodulatory drugs	383:434	various anti-inflammatory and immunomodulatory drugs	383:434	Despite the availability of various anti-inflammatory and immunomodulatory drugs, many patients fail to respond to pharmacologic therapy and some experience drug-induced adverse events.
34199466	3	32	theme	drug-induced	512:523	arg1	events					533:538	drug-induced adverse events	512:538	drug-induced adverse events	512:538	Despite the availability of various anti-inflammatory and immunomodulatory drugs, many patients fail to respond to pharmacologic therapy and some experience drug-induced adverse events.
34199466	9	33	theme	diversity	1435:1443	arg1	visualization					1402:1414	visualization	1402:1414	visualization of the Bray-Curtis diversity by principal coordinates analysis (PCoA)	1402:1484	The gut microbiome analysis revealed distinct clusters in the saffron-treated and untreated mice in dextran sulfate sodium (DSS)-induced colitis by visualization of the Bray-Curtis diversity by principal coordinates analysis (PCoA).
34199466	9	34	theme	principal	1448:1456	arg1	PCoA					1480:1483	PCoA	1480:1483	PCoA	1480:1483	The gut microbiome analysis revealed distinct clusters in the saffron-treated and untreated mice in dextran sulfate sodium (DSS)-induced colitis by visualization of the Bray-Curtis diversity by principal coordinates analysis (PCoA).
34199466	9	34	theme	principal	1448:1456	arg1	analysis					1470:1477	principal coordinates analysis	1448:1477	principal coordinates analysis (PCoA)	1448:1484	The gut microbiome analysis revealed distinct clusters in the saffron-treated and untreated mice in dextran sulfate sodium (DSS)-induced colitis by visualization of the Bray-Curtis diversity by principal coordinates analysis (PCoA).
34199466	4	35	theme	Crocus	584:589	arg1	saffron					575:581	saffron	575:581	particularly saffron (Crocus sativus)	562:598	Dietary supplements, particularly saffron (Crocus sativus), have recently gained an appreciable attention in alleviating some symptoms of digestive diseases.
34199466	4	35	theme	Crocus	584:589	arg1	sativus					591:597	Crocus sativus	584:597	Crocus sativus	584:597	Dietary supplements, particularly saffron (Crocus sativus), have recently gained an appreciable attention in alleviating some symptoms of digestive diseases.
34199466	0	36	theme	Inflammatory	51:62	arg1	Profiles					64:71	Tissue Inflammatory Profiles	44:71	Tissue Inflammatory Profiles	44:71	Saffron Pre-Treatment Promotes Reduction in Tissue Inflammatory Profiles and Alters Microbiome Composition in Experimental Colitis Mice.
34199466	1	37	with	condition	196:204	arg1	pathogenesis					268:279	an incompletely understood pathogenesis	241:279	an incompletely understood pathogenesis	241:279	Inflammatory bowel disease (IBD) is a chronic inflammatory condition of the gastrointestinal tract with an incompletely understood pathogenesis.
34199466	6	38	theme	experimental	914:925	arg1	colitis					927:933	murine experimental colitis	907:933	murine experimental colitis	907:933	Saffron pre-treatment improved the gross and histopathological characteristics of the colonic mucosa in murine experimental colitis.
34199466	6	39	theme	histopathological	848:864	arg1	characteristics					866:880	the gross and histopathological characteristics	834:880	the gross and histopathological characteristics of the colonic mucosa in murine experimental colitis	834:933	Saffron pre-treatment improved the gross and histopathological characteristics of the colonic mucosa in murine experimental colitis.
34199466	7	40	with	Treatment	936:944	arg1	saffron					951:957	saffron	951:957	saffron	951:957	Treatment with saffron showed a significant amelioration of colitis when compared to the vehicle-treated mice group.
34199466	8	41	theme	Saffron	1053:1059	arg1	treatment					1061:1069	Saffron treatment	1053:1069	Saffron treatment	1053:1069	Saffron treatment significantly decreased secretion of serotonin and pro-inflammatory cytokines, such as TNF-α, IL-1β, and IL-6, in the colon tissues by suppressing the nuclear translocation of NF-κB.
34199466	10	42	theme	fatty	1612:1616	arg1	acids					1618:1622	short-chain fatty acids	1600:1622	short-chain fatty acids (SCFAs)	1600:1630	Furthermore, we observed that, at the operational taxonomic unit (OTU) level, Cyanobacteria were depleted, while short-chain fatty acids (SCFAs), such as isobutyric acid, acetic acid, and propionic acid, were increased in saffron-treated mice.
34199466	10	42	theme	fatty	1612:1616	arg1	acid					1652:1655	isobutyric acid	1641:1655	isobutyric acid	1641:1655	Furthermore, we observed that, at the operational taxonomic unit (OTU) level, Cyanobacteria were depleted, while short-chain fatty acids (SCFAs), such as isobutyric acid, acetic acid, and propionic acid, were increased in saffron-treated mice.
34199466	10	42	theme	fatty	1612:1616	arg1	acid					1685:1688	propionic acid	1675:1688	propionic acid	1675:1688	Furthermore, we observed that, at the operational taxonomic unit (OTU) level, Cyanobacteria were depleted, while short-chain fatty acids (SCFAs), such as isobutyric acid, acetic acid, and propionic acid, were increased in saffron-treated mice.
34199466	10	42	theme	fatty	1612:1616	arg1	SCFAs					1625:1629	SCFAs	1625:1629	SCFAs	1625:1629	Furthermore, we observed that, at the operational taxonomic unit (OTU) level, Cyanobacteria were depleted, while short-chain fatty acids (SCFAs), such as isobutyric acid, acetic acid, and propionic acid, were increased in saffron-treated mice.
34199466	10	42	theme	fatty	1612:1616	arg1	acid					1665:1668	acetic acid	1658:1668	acetic acid	1658:1668	Furthermore, we observed that, at the operational taxonomic unit (OTU) level, Cyanobacteria were depleted, while short-chain fatty acids (SCFAs), such as isobutyric acid, acetic acid, and propionic acid, were increased in saffron-treated mice.
34199466	7	43	theme	significant	968:978	arg1	amelioration					980:991	a significant amelioration	966:991	a significant amelioration of colitis	966:1002	Treatment with saffron showed a significant amelioration of colitis when compared to the vehicle-treated mice group.
34199466	8	44	theme	nuclear	1222:1228	arg1	translocation					1230:1242	the nuclear translocation	1218:1242	the nuclear translocation of NF-κB	1218:1251	Saffron treatment significantly decreased secretion of serotonin and pro-inflammatory cytokines, such as TNF-α, IL-1β, and IL-6, in the colon tissues by suppressing the nuclear translocation of NF-κB.
34199466	4	45	theme	appreciable	625:635	arg1	attention					637:645	an appreciable attention	622:645	an appreciable attention	622:645	Dietary supplements, particularly saffron (Crocus sativus), have recently gained an appreciable attention in alleviating some symptoms of digestive diseases.
34199466	9	46	theme	microbiome	1262:1271	arg1	analysis					1273:1280	The gut microbiome analysis	1254:1280	The gut microbiome analysis	1254:1280	The gut microbiome analysis revealed distinct clusters in the saffron-treated and untreated mice in dextran sulfate sodium (DSS)-induced colitis by visualization of the Bray-Curtis diversity by principal coordinates analysis (PCoA).
34199466	2	47	theme	increased	323:331	arg1	risk					333:336	increased risk	323:336	increased risk of colon cancer	323:352	Long-standing colitis is associated with increased risk of colon cancer.
34199466	11	48	with	pre-treatment	1753:1765	arg1	saffron					1772:1778	saffron	1772:1778	saffron	1772:1778	Our data suggest that pre-treatment with saffron inhibits DSS-induced pro-inflammatory cytokine secretion, modulates gut microbiota composition, prevents the depletion of SCFAs, and reduces the susceptibility to colitis.
34199466	8	49	theme	serotonin	1108:1116	arg1	secretion					1095:1103	secretion	1095:1103	secretion of serotonin and pro-inflammatory cytokines, such as TNF-α, IL-1β, and IL-6, in the colon tissues	1095:1201	Saffron treatment significantly decreased secretion of serotonin and pro-inflammatory cytokines, such as TNF-α, IL-1β, and IL-6, in the colon tissues by suppressing the nuclear translocation of NF-κB.
34199466	2	50	theme	cancer	347:352	arg1	risk					333:336	increased risk	323:336	increased risk of colon cancer	323:352	Long-standing colitis is associated with increased risk of colon cancer.
34199466	1	51	theme	inflammatory	183:194	arg1	condition					196:204	a chronic inflammatory condition	173:204	a chronic inflammatory condition of the gastrointestinal tract with an incompletely understood pathogenesis	173:279	Inflammatory bowel disease (IBD) is a chronic inflammatory condition of the gastrointestinal tract with an incompletely understood pathogenesis.
34199466	1	51	theme	inflammatory	183:194	arg1	disease					156:162	Inflammatory bowel disease	137:162	Inflammatory bowel disease (IBD)	137:168	Inflammatory bowel disease (IBD) is a chronic inflammatory condition of the gastrointestinal tract with an incompletely understood pathogenesis.
34199466	8	52	theme	pro-inflammatory	1122:1137	arg1	cytokines					1139:1147	pro-inflammatory cytokines	1122:1147	pro-inflammatory cytokines	1122:1147	Saffron treatment significantly decreased secretion of serotonin and pro-inflammatory cytokines, such as TNF-α, IL-1β, and IL-6, in the colon tissues by suppressing the nuclear translocation of NF-κB.
34199466	8	52	theme	pro-inflammatory	1122:1137	arg1	IL-1β					1165:1169	IL-1β	1165:1169	IL-1β	1165:1169	Saffron treatment significantly decreased secretion of serotonin and pro-inflammatory cytokines, such as TNF-α, IL-1β, and IL-6, in the colon tissues by suppressing the nuclear translocation of NF-κB.
34199466	8	52	theme	pro-inflammatory	1122:1137	arg1	IL-6					1176:1179	IL-6	1176:1179	IL-6	1176:1179	Saffron treatment significantly decreased secretion of serotonin and pro-inflammatory cytokines, such as TNF-α, IL-1β, and IL-6, in the colon tissues by suppressing the nuclear translocation of NF-κB.
34199466	8	52	theme	pro-inflammatory	1122:1137	arg1	TNF-α					1158:1162	TNF-α	1158:1162	TNF-α	1158:1162	Saffron treatment significantly decreased secretion of serotonin and pro-inflammatory cytokines, such as TNF-α, IL-1β, and IL-6, in the colon tissues by suppressing the nuclear translocation of NF-κB.
34199466	10	53	theme	isobutyric	1641:1650	arg1	acid					1652:1655	isobutyric acid	1641:1655	isobutyric acid	1641:1655	Furthermore, we observed that, at the operational taxonomic unit (OTU) level, Cyanobacteria were depleted, while short-chain fatty acids (SCFAs), such as isobutyric acid, acetic acid, and propionic acid, were increased in saffron-treated mice.
34199466	0	54	from	Composition	95:105	arg1	Mice					131:134	Experimental Colitis Mice	110:134	Experimental Colitis Mice	110:134	Saffron Pre-Treatment Promotes Reduction in Tissue Inflammatory Profiles and Alters Microbiome Composition in Experimental Colitis Mice.
34199466	4	55	theme	diseases	689:696	arg1	symptoms					667:674	some symptoms	662:674	some symptoms of digestive diseases	662:696	Dietary supplements, particularly saffron (Crocus sativus), have recently gained an appreciable attention in alleviating some symptoms of digestive diseases.
34199466	1	56	theme	gastrointestinal	213:228	arg1	tract					230:234	the gastrointestinal tract	209:234	the gastrointestinal tract	209:234	Inflammatory bowel disease (IBD) is a chronic inflammatory condition of the gastrointestinal tract with an incompletely understood pathogenesis.
34199466	11	57	theme	SCFAs	1902:1906	arg1	depletion					1889:1897	the depletion	1885:1897	the depletion of SCFAs	1885:1906	Our data suggest that pre-treatment with saffron inhibits DSS-induced pro-inflammatory cytokine secretion, modulates gut microbiota composition, prevents the depletion of SCFAs, and reduces the susceptibility to colitis.
34199466	1	58	theme	bowel	150:154	arg1	IBD					165:167	IBD	165:167	IBD	165:167	Inflammatory bowel disease (IBD) is a chronic inflammatory condition of the gastrointestinal tract with an incompletely understood pathogenesis.
34199466	1	58	theme	bowel	150:154	arg1	disease					156:162	Inflammatory bowel disease	137:162	Inflammatory bowel disease (IBD)	137:168	Inflammatory bowel disease (IBD) is a chronic inflammatory condition of the gastrointestinal tract with an incompletely understood pathogenesis.
34199466	1	58	theme	bowel	150:154	arg1	condition					196:204	a chronic inflammatory condition	173:204	a chronic inflammatory condition of the gastrointestinal tract with an incompletely understood pathogenesis	173:279	Inflammatory bowel disease (IBD) is a chronic inflammatory condition of the gastrointestinal tract with an incompletely understood pathogenesis.
34199466	9	59	from	clusters	1300:1307	arg1	mice					1346:1349	the saffron-treated and untreated mice	1312:1349	the saffron-treated and untreated mice	1312:1349	The gut microbiome analysis revealed distinct clusters in the saffron-treated and untreated mice in dextran sulfate sodium (DSS)-induced colitis by visualization of the Bray-Curtis diversity by principal coordinates analysis (PCoA).
34199466	11	60	theme	cytokine	1818:1825	arg1	secretion					1827:1835	DSS-induced pro-inflammatory cytokine secretion	1789:1835	DSS-induced pro-inflammatory cytokine secretion	1789:1835	Our data suggest that pre-treatment with saffron inhibits DSS-induced pro-inflammatory cytokine secretion, modulates gut microbiota composition, prevents the depletion of SCFAs, and reduces the susceptibility to colitis.
34199466	0	61	from	Reduction	31:39	arg1	Profiles					64:71	Tissue Inflammatory Profiles	44:71	Tissue Inflammatory Profiles	44:71	Saffron Pre-Treatment Promotes Reduction in Tissue Inflammatory Profiles and Alters Microbiome Composition in Experimental Colitis Mice.
34199466	11	62	theme	DSS-induced	1789:1799	arg1	secretion					1827:1835	DSS-induced pro-inflammatory cytokine secretion	1789:1835	DSS-induced pro-inflammatory cytokine secretion	1789:1835	Our data suggest that pre-treatment with saffron inhibits DSS-induced pro-inflammatory cytokine secretion, modulates gut microbiota composition, prevents the depletion of SCFAs, and reduces the susceptibility to colitis.
34199466	11	63	theme	gut	1848:1850	arg1	composition					1863:1873	gut microbiota composition	1848:1873	gut microbiota composition	1848:1873	Our data suggest that pre-treatment with saffron inhibits DSS-induced pro-inflammatory cytokine secretion, modulates gut microbiota composition, prevents the depletion of SCFAs, and reduces the susceptibility to colitis.
34199466	0	64	theme	Experimental	110:121	arg1	Mice					131:134	Experimental Colitis Mice	110:134	Experimental Colitis Mice	110:134	Saffron Pre-Treatment Promotes Reduction in Tissue Inflammatory Profiles and Alters Microbiome Composition in Experimental Colitis Mice.
34199466	9	65	theme	-induced	1382:1389	arg1	colitis					1391:1397	dextran sulfate sodium (DSS)-induced colitis	1354:1397	dextran sulfate sodium (DSS)-induced colitis	1354:1397	The gut microbiome analysis revealed distinct clusters in the saffron-treated and untreated mice in dextran sulfate sodium (DSS)-induced colitis by visualization of the Bray-Curtis diversity by principal coordinates analysis (PCoA).
34199466	5	66	theme	colitis	788:794	arg1	model					796:800	a murine colitis model	779:800	a murine colitis model	779:800	In our study, we investigated whether saffron may have a prophylactic effect in a murine colitis model.
34199466	3	67	theme	various	383:389	arg1	drugs					430:434	various anti-inflammatory and immunomodulatory drugs	383:434	various anti-inflammatory and immunomodulatory drugs	383:434	Despite the availability of various anti-inflammatory and immunomodulatory drugs, many patients fail to respond to pharmacologic therapy and some experience drug-induced adverse events.
34199466	7	68	theme	vehicle-treated	1025:1039	arg1	group					1046:1050	the vehicle-treated mice group	1021:1050	the vehicle-treated mice group	1021:1050	Treatment with saffron showed a significant amelioration of colitis when compared to the vehicle-treated mice group.
34199466	3	69	theme	many	437:440	arg1	patients					442:449	many patients	437:449	many patients	437:449	Despite the availability of various anti-inflammatory and immunomodulatory drugs, many patients fail to respond to pharmacologic therapy and some experience drug-induced adverse events.
34199466	10	70	theme	taxonomic	1537:1545	arg1	level					1558:1562	the operational taxonomic unit (OTU) level	1521:1562	the operational taxonomic unit (OTU) level	1521:1562	Furthermore, we observed that, at the operational taxonomic unit (OTU) level, Cyanobacteria were depleted, while short-chain fatty acids (SCFAs), such as isobutyric acid, acetic acid, and propionic acid, were increased in saffron-treated mice.
34199466	3	71	theme	pharmacologic	470:482	arg1	therapy					484:490	pharmacologic therapy	470:490	pharmacologic therapy	470:490	Despite the availability of various anti-inflammatory and immunomodulatory drugs, many patients fail to respond to pharmacologic therapy and some experience drug-induced adverse events.
34199466	9	72	theme	Bray-Curtis	1423:1433	arg1	diversity					1435:1443	the Bray-Curtis diversity	1419:1443	the Bray-Curtis diversity	1419:1443	The gut microbiome analysis revealed distinct clusters in the saffron-treated and untreated mice in dextran sulfate sodium (DSS)-induced colitis by visualization of the Bray-Curtis diversity by principal coordinates analysis (PCoA).
34199466	3	73	theme	drugs	430:434	arg1	availability					367:378	the availability	363:378	the availability of various anti-inflammatory and immunomodulatory drugs	363:434	Despite the availability of various anti-inflammatory and immunomodulatory drugs, many patients fail to respond to pharmacologic therapy and some experience drug-induced adverse events.
34199466	10	74	theme	saffron-treated	1709:1723	arg1	mice					1725:1728	saffron-treated mice	1709:1728	saffron-treated mice	1709:1728	Furthermore, we observed that, at the operational taxonomic unit (OTU) level, Cyanobacteria were depleted, while short-chain fatty acids (SCFAs), such as isobutyric acid, acetic acid, and propionic acid, were increased in saffron-treated mice.
34199466	0	75	theme	Tissue	44:49	arg1	Profiles					64:71	Tissue Inflammatory Profiles	44:71	Tissue Inflammatory Profiles	44:71	Saffron Pre-Treatment Promotes Reduction in Tissue Inflammatory Profiles and Alters Microbiome Composition in Experimental Colitis Mice.
34199466	5	76	contain	have	749:752	arg2	effect					769:774	a prophylactic effect	754:774	a prophylactic effect	754:774	In our study, we investigated whether saffron may have a prophylactic effect in a murine colitis model.
34199466	5	76	contain	have	749:752	arg1	saffron					737:743	saffron	737:743	saffron	737:743	In our study, we investigated whether saffron may have a prophylactic effect in a murine colitis model.
34199466	9	77	theme	coordinates	1458:1468	arg1	PCoA					1480:1483	PCoA	1480:1483	PCoA	1480:1483	The gut microbiome analysis revealed distinct clusters in the saffron-treated and untreated mice in dextran sulfate sodium (DSS)-induced colitis by visualization of the Bray-Curtis diversity by principal coordinates analysis (PCoA).
34199466	9	77	theme	coordinates	1458:1468	arg1	analysis					1470:1477	principal coordinates analysis	1448:1477	principal coordinates analysis (PCoA)	1448:1484	The gut microbiome analysis revealed distinct clusters in the saffron-treated and untreated mice in dextran sulfate sodium (DSS)-induced colitis by visualization of the Bray-Curtis diversity by principal coordinates analysis (PCoA).
34199466	8	78	theme	colon	1189:1193	arg1	tissues					1195:1201	the colon tissues	1185:1201	the colon tissues	1185:1201	Saffron treatment significantly decreased secretion of serotonin and pro-inflammatory cytokines, such as TNF-α, IL-1β, and IL-6, in the colon tissues by suppressing the nuclear translocation of NF-κB.
34199466	8	79	from	secretion	1095:1103	arg1	tissues					1195:1201	the colon tissues	1185:1201	the colon tissues	1185:1201	Saffron treatment significantly decreased secretion of serotonin and pro-inflammatory cytokines, such as TNF-α, IL-1β, and IL-6, in the colon tissues by suppressing the nuclear translocation of NF-κB.
34199466	6	80	theme	Saffron	803:809	arg1	pre-treatment					811:823	Saffron pre-treatment	803:823	Saffron pre-treatment	803:823	Saffron pre-treatment improved the gross and histopathological characteristics of the colonic mucosa in murine experimental colitis.
34199466	7	81	theme	colitis	996:1002	arg1	amelioration					980:991	a significant amelioration	966:991	a significant amelioration of colitis	966:1002	Treatment with saffron showed a significant amelioration of colitis when compared to the vehicle-treated mice group.
34199466	6	82	theme	murine	907:912	arg1	colitis					927:933	murine experimental colitis	907:933	murine experimental colitis	907:933	Saffron pre-treatment improved the gross and histopathological characteristics of the colonic mucosa in murine experimental colitis.
34199466	6	83	theme	mucosa	897:902	arg1	characteristics					866:880	the gross and histopathological characteristics	834:880	the gross and histopathological characteristics of the colonic mucosa in murine experimental colitis	834:933	Saffron pre-treatment improved the gross and histopathological characteristics of the colonic mucosa in murine experimental colitis.
34199466	4	84	theme	digestive	679:687	arg1	diseases					689:696	digestive diseases	679:696	digestive diseases	679:696	Dietary supplements, particularly saffron (Crocus sativus), have recently gained an appreciable attention in alleviating some symptoms of digestive diseases.
34199466	1	85	theme	understood	257:266	arg1	pathogenesis					268:279	an incompletely understood pathogenesis	241:279	an incompletely understood pathogenesis	241:279	Inflammatory bowel disease (IBD) is a chronic inflammatory condition of the gastrointestinal tract with an incompletely understood pathogenesis.
33529230	10	0	theme	monomeric	1801:1809	arg1	content					1816:1822	the monomeric form content	1797:1822	the monomeric form content	1797:1822	Purified proteins were analyzed by polyacrylamide gel electrophoresis in reducing and non-reducing conditions and gel filtration; for RBDv2 protein, the monomeric form content exceeded 90% for several series.
33529230	1	1	theme	viral	218:222	arg1	envelope					224:231	the coronaviruses' viral envelope	199:231	the coronaviruses' viral envelope	199:231	The spike (S) protein is one of the three proteins forming the coronaviruses' viral envelope.
33529230	7	2	theme	genetic	1395:1401	arg1	cassette					1403:1410	the genetic cassette	1391:1410	the genetic cassette	1391:1410	We transfected a CHO DG44 cell line, selected stably transfected cells, and performed a few rounds of methotrexate-driven amplification of the genetic cassette in the genome.
33529230	10	3	theme	gel	1698:1700	arg1	electrophoresis					1702:1716	polyacrylamide gel electrophoresis	1683:1716	polyacrylamide gel electrophoresis	1683:1716	Purified proteins were analyzed by polyacrylamide gel electrophoresis in reducing and non-reducing conditions and gel filtration; for RBDv2 protein, the monomeric form content exceeded 90% for several series.
33529230	7	4	theme	few	1340:1342	arg1	rounds					1344:1349	a few rounds	1338:1349	a few rounds of methotrexate-driven amplification of the genetic cassette in the genome	1338:1424	We transfected a CHO DG44 cell line, selected stably transfected cells, and performed a few rounds of methotrexate-driven amplification of the genetic cassette in the genome.
33529230	6	5	theme	viral	1197:1201	arg1	peptide					1210:1216	a native viral signal peptide	1188:1216	a native viral signal peptide	1188:1216	RBDv1 contained a native viral signal peptide, RBDv2 -human tPA signal peptide.
33529230	6	5	theme	viral	1197:1201	arg1	peptide					1243:1249	RBDv2 -human tPA signal peptide	1219:1249	RBDv2 -human tPA signal peptide	1219:1249	RBDv1 contained a native viral signal peptide, RBDv2 -human tPA signal peptide.
33529230	5	6	theme	c-myc	1142:1146	arg1	tags					1166:1169	C-terminal c-myc and polyhistidine tags	1131:1169	C-terminal c-myc and polyhistidine tags	1131:1169	Based on the previously developed p1.1 vector, containing the regulatory sequences of the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1) from Chinese hamster, we created two expression constructs encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine tags.
33529230	4	7	theme	antigenic	770:778	arg1	glycans					795:801	no potentially antigenic non-vertebrate glycans	755:801	no potentially antigenic non-vertebrate glycans	755:801	Correct exposure of antigenic determinants has a significant impact on the accuracy of such tests-the antigen has to be correctly folded, contain no potentially antigenic non-vertebrate glycans, and, preferably, should have a glycosylation pattern similar to the native S protein.
33529230	12	8	theme	subunit	2038:2044	arg1	studies					2054:2060	subunit vaccine studies	2038:2060	subunit vaccine studies	2038:2060	The antigen produced by the described technique is suitable for serological tests and subunit vaccine studies.
33529230	7	9	theme	methotrexate-driven	1354:1372	arg1	amplification					1374:1386	methotrexate-driven amplification	1354:1386	methotrexate-driven amplification of the genetic cassette in the genome	1354:1424	We transfected a CHO DG44 cell line, selected stably transfected cells, and performed a few rounds of methotrexate-driven amplification of the genetic cassette in the genome.
33529230	9	10	theme	simple	1615:1620	arg1	culture					1639:1645	the simple shake flask cell culture	1611:1645	the simple shake flask cell culture	1611:1645	We developed a simple purification scheme that consistently yielded up to 30 mg of RBD protein per liter of the simple shake flask cell culture.
33529230	2	11	theme	Acute	262:266	arg1	Coronavirus					289:299	the Severe Acute Respiratory Syndrome Coronavirus 2	251:301	the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)	251:314	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	2	11	theme	Acute	262:266	arg1	SARS-CoV-2					304:313	SARS-CoV-2	304:313	SARS-CoV-2	304:313	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	7	12	from	amplification	1374:1386	arg1	genome					1419:1424	the genome	1415:1424	the genome	1415:1424	We transfected a CHO DG44 cell line, selected stably transfected cells, and performed a few rounds of methotrexate-driven amplification of the genetic cassette in the genome.
33529230	9	13	theme	protein	1590:1596	arg1	mg					1580:1581	up to 30 mg	1571:1581	up to 30 mg of RBD protein per liter of the simple shake flask cell culture	1571:1645	We developed a simple purification scheme that consistently yielded up to 30 mg of RBD protein per liter of the simple shake flask cell culture.
33529230	10	14	theme	polyacrylamide	1683:1696	arg1	electrophoresis					1702:1716	polyacrylamide gel electrophoresis	1683:1716	polyacrylamide gel electrophoresis	1683:1716	Purified proteins were analyzed by polyacrylamide gel electrophoresis in reducing and non-reducing conditions and gel filtration; for RBDv2 protein, the monomeric form content exceeded 90% for several series.
33529230	7	15	theme	transfected	1305:1315	arg1	cells					1317:1321	stably transfected cells	1298:1321	stably transfected cells	1298:1321	We transfected a CHO DG44 cell line, selected stably transfected cells, and performed a few rounds of methotrexate-driven amplification of the genetic cassette in the genome.
33529230	5	16	theme	translation	991:1001	arg1	factor					1014:1019	Eukaryotic translation elongation factor 1	980:1021	the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1)	976:1041	Based on the previously developed p1.1 vector, containing the regulatory sequences of the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1) from Chinese hamster, we created two expression constructs encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine tags.
33529230	2	17	theme	significant	462:472	arg1	inducer					474:480	a significant inducer	460:480	a significant inducer of host immune response	460:504	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	2	17	theme	significant	462:472	arg1	domain					437:442	a unique receptor-binding domain	411:442	a unique receptor-binding domain (RBD)	411:448	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	0	18	theme	plasmid	124:130	arg1	vector					132:137	the EEF1A1-based plasmid vector	107:137	the EEF1A1-based plasmid vector	107:137	High-level expression of the monomeric SARS-CoV-2 S protein RBD 320-537 in stably transfected CHO cells by the EEF1A1-based plasmid vector.
33529230	5	19	theme	factor	1014:1019	arg1	EEF1A1					1035:1040	EEF1A1	1035:1040	EEF1A1	1035:1040	Based on the previously developed p1.1 vector, containing the regulatory sequences of the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1) from Chinese hamster, we created two expression constructs encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine tags.
33529230	5	19	theme	factor	1014:1019	arg1	gene					1029:1032	the Eukaryotic translation elongation factor 1 alpha gene	976:1032	the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1)	976:1041	Based on the previously developed p1.1 vector, containing the regulatory sequences of the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1) from Chinese hamster, we created two expression constructs encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine tags.
33529230	9	20	theme	shake	1622:1626	arg1	culture					1639:1645	the simple shake flask cell culture	1611:1645	the simple shake flask cell culture	1611:1645	We developed a simple purification scheme that consistently yielded up to 30 mg of RBD protein per liter of the simple shake flask cell culture.
33529230	12	21	theme	described	1980:1988	arg1	technique					1990:1998	the described technique	1976:1998	the described technique	1976:1998	The antigen produced by the described technique is suitable for serological tests and subunit vaccine studies.
33529230	6	22	theme	tPA	1232:1234	arg1	peptide					1210:1216	a native viral signal peptide	1188:1216	a native viral signal peptide	1188:1216	RBDv1 contained a native viral signal peptide, RBDv2 -human tPA signal peptide.
33529230	6	22	theme	tPA	1232:1234	arg1	peptide					1243:1249	RBDv2 -human tPA signal peptide	1219:1249	RBDv2 -human tPA signal peptide	1219:1249	RBDv1 contained a native viral signal peptide, RBDv2 -human tPA signal peptide.
33529230	3	23	theme	SARS-CoV-2	519:528	arg1	RBD					530:532	Recombinant SARS-CoV-2 RBD	507:532	Recombinant SARS-CoV-2 RBD	507:532	Recombinant SARS-CoV-2 RBD is widely used as a highly specific minimal antigen for serological tests.
33529230	3	23	theme	SARS-CoV-2	519:528	arg1	antigen					578:584	a highly specific minimal antigen	552:584	a highly specific minimal antigen for serological tests	552:606	Recombinant SARS-CoV-2 RBD is widely used as a highly specific minimal antigen for serological tests.
33529230	2	24	theme	immune	490:495	arg1	response					497:504	host immune response	485:504	host immune response	485:504	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	7	25	theme	DG44	1273:1276	arg1	line					1283:1286	a CHO DG44 cell line	1267:1286	a CHO DG44 cell line	1267:1286	We transfected a CHO DG44 cell line, selected stably transfected cells, and performed a few rounds of methotrexate-driven amplification of the genetic cassette in the genome.
33529230	4	26	theme	significant	658:668	arg1	impact					670:675	a significant impact	656:675	a significant impact	656:675	Correct exposure of antigenic determinants has a significant impact on the accuracy of such tests-the antigen has to be correctly folded, contain no potentially antigenic non-vertebrate glycans, and, preferably, should have a glycosylation pattern similar to the native S protein.
33529230	6	27	theme	RBDv2	1219:1223	arg1	peptide					1210:1216	a native viral signal peptide	1188:1216	a native viral signal peptide	1188:1216	RBDv1 contained a native viral signal peptide, RBDv2 -human tPA signal peptide.
33529230	6	27	theme	RBDv2	1219:1223	arg1	peptide					1243:1249	RBDv2 -human tPA signal peptide	1219:1249	RBDv2 -human tPA signal peptide	1219:1249	RBDv1 contained a native viral signal peptide, RBDv2 -human tPA signal peptide.
33529230	10	28	theme	non-reducing	1734:1745	arg1	conditions					1747:1756	non-reducing conditions	1734:1756	non-reducing conditions	1734:1756	Purified proteins were analyzed by polyacrylamide gel electrophoresis in reducing and non-reducing conditions and gel filtration; for RBDv2 protein, the monomeric form content exceeded 90% for several series.
33529230	9	29	theme	cell	1634:1637	arg1	culture					1639:1645	the simple shake flask cell culture	1611:1645	the simple shake flask cell culture	1611:1645	We developed a simple purification scheme that consistently yielded up to 30 mg of RBD protein per liter of the simple shake flask cell culture.
33529230	3	30	used	used	544:547	arg2	antigen					578:584	a highly specific minimal antigen	552:584	a highly specific minimal antigen for serological tests	552:606	Recombinant SARS-CoV-2 RBD is widely used as a highly specific minimal antigen for serological tests.
33529230	3	30	used	used	544:547	arg2	RBD					530:532	Recombinant SARS-CoV-2 RBD	507:532	Recombinant SARS-CoV-2 RBD	507:532	Recombinant SARS-CoV-2 RBD is widely used as a highly specific minimal antigen for serological tests.
33529230	0	31	theme	RBD	60:62	arg1	320-537					64:70	protein RBD 320-537	52:70	protein RBD 320-537 in stably transfected CHO cells	52:102	High-level expression of the monomeric SARS-CoV-2 S protein RBD 320-537 in stably transfected CHO cells by the EEF1A1-based plasmid vector.
33529230	11	32	theme	PNGase	1878:1883	arg1	F					1885:1885	PNGase F	1878:1885	PNGase F	1878:1885	Deglycosylation with PNGase F and mass spectrometry confirmed the presence of N-glycosylation.
33529230	9	33	theme	flask	1628:1632	arg1	culture					1639:1645	the simple shake flask cell culture	1611:1645	the simple shake flask cell culture	1611:1645	We developed a simple purification scheme that consistently yielded up to 30 mg of RBD protein per liter of the simple shake flask cell culture.
33529230	4	34	theme	antigenic	629:637	arg1	determinants					639:650	antigenic determinants	629:650	antigenic determinants	629:650	Correct exposure of antigenic determinants has a significant impact on the accuracy of such tests-the antigen has to be correctly folded, contain no potentially antigenic non-vertebrate glycans, and, preferably, should have a glycosylation pattern similar to the native S protein.
33529230	4	35	contain	contain	747:753	arg2	glycans					795:801	no potentially antigenic non-vertebrate glycans	755:801	no potentially antigenic non-vertebrate glycans	755:801	Correct exposure of antigenic determinants has a significant impact on the accuracy of such tests-the antigen has to be correctly folded, contain no potentially antigenic non-vertebrate glycans, and, preferably, should have a glycosylation pattern similar to the native S protein.
33529230	4	35	contain	contain	747:753	arg1	exposure					617:624	Correct exposure	609:624	Correct exposure of antigenic determinants	609:650	Correct exposure of antigenic determinants has a significant impact on the accuracy of such tests-the antigen has to be correctly folded, contain no potentially antigenic non-vertebrate glycans, and, preferably, should have a glycosylation pattern similar to the native S protein.
33529230	5	36	theme	Chinese	1048:1054	arg1	hamster					1056:1062	Chinese hamster	1048:1062	Chinese hamster	1048:1062	Based on the previously developed p1.1 vector, containing the regulatory sequences of the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1) from Chinese hamster, we created two expression constructs encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine tags.
33529230	4	37	theme	native	872:877	arg1	protein					881:887	the native S protein	868:887	the native S protein	868:887	Correct exposure of antigenic determinants has a significant impact on the accuracy of such tests-the antigen has to be correctly folded, contain no potentially antigenic non-vertebrate glycans, and, preferably, should have a glycosylation pattern similar to the native S protein.
33529230	4	38	theme	such	696:699	arg1	antigen					711:717	such tests-the antigen	696:717	such tests-the antigen has to be correctly folded	696:744	Correct exposure of antigenic determinants has a significant impact on the accuracy of such tests-the antigen has to be correctly folded, contain no potentially antigenic non-vertebrate glycans, and, preferably, should have a glycosylation pattern similar to the native S protein.
33529230	0	39	theme	transfected	82:92	arg1	cells					98:102	stably transfected CHO cells	75:102	stably transfected CHO cells	75:102	High-level expression of the monomeric SARS-CoV-2 S protein RBD 320-537 in stably transfected CHO cells by the EEF1A1-based plasmid vector.
33529230	5	40	theme	alpha	1023:1027	arg1	EEF1A1					1035:1040	EEF1A1	1035:1040	EEF1A1	1035:1040	Based on the previously developed p1.1 vector, containing the regulatory sequences of the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1) from Chinese hamster, we created two expression constructs encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine tags.
33529230	5	40	theme	alpha	1023:1027	arg1	gene					1029:1032	the Eukaryotic translation elongation factor 1 alpha gene	976:1032	the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1)	976:1041	Based on the previously developed p1.1 vector, containing the regulatory sequences of the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1) from Chinese hamster, we created two expression constructs encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine tags.
33529230	4	41	contain	have	828:831	arg2	pattern					849:855	a glycosylation pattern	833:855	a glycosylation pattern similar to the native S protein	833:887	Correct exposure of antigenic determinants has a significant impact on the accuracy of such tests-the antigen has to be correctly folded, contain no potentially antigenic non-vertebrate glycans, and, preferably, should have a glycosylation pattern similar to the native S protein.
33529230	4	41	contain	have	828:831	arg1	exposure					617:624	Correct exposure	609:624	Correct exposure of antigenic determinants	609:650	Correct exposure of antigenic determinants has a significant impact on the accuracy of such tests-the antigen has to be correctly folded, contain no potentially antigenic non-vertebrate glycans, and, preferably, should have a glycosylation pattern similar to the native S protein.
33529230	4	42	theme	antigen	711:717	arg1	accuracy					684:691	the accuracy	680:691	the accuracy of such tests-the antigen has to be correctly folded	680:744	Correct exposure of antigenic determinants has a significant impact on the accuracy of such tests-the antigen has to be correctly folded, contain no potentially antigenic non-vertebrate glycans, and, preferably, should have a glycosylation pattern similar to the native S protein.
33529230	11	43	with	Deglycosylation	1857:1871	arg1	F					1885:1885	PNGase F	1878:1885	PNGase F	1878:1885	Deglycosylation with PNGase F and mass spectrometry confirmed the presence of N-glycosylation.
33529230	11	43	with	Deglycosylation	1857:1871	arg1	spectrometry					1896:1907	mass spectrometry	1891:1907	mass spectrometry	1891:1907	Deglycosylation with PNGase F and mass spectrometry confirmed the presence of N-glycosylation.
33529230	2	44	contain	has	316:318	arg1	protein					240:246	The S protein	234:246	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)	234:314	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	2	44	contain	has	316:318	arg2	structure					330:338	a spatial structure	320:338	a spatial structure similar to the S proteins of other mammalian coronaviruses	320:397	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	1	45	dep	spike	144:148	arg1	S					151:151	S	151:151	S	151:151	The spike (S) protein is one of the three proteins forming the coronaviruses' viral envelope.
33529230	0	46	theme	High-level	0:9	arg1	expression					11:20	High-level expression	0:20	High-level expression of the monomeric SARS-CoV-2	0:48	High-level expression of the monomeric SARS-CoV-2 S protein RBD 320-537 in stably transfected CHO cells by the EEF1A1-based plasmid vector.
33529230	5	47	theme	expression	1080:1089	arg1	constructs					1091:1100	two expression constructs	1076:1100	two expression constructs encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine tags	1076:1169	Based on the previously developed p1.1 vector, containing the regulatory sequences of the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1) from Chinese hamster, we created two expression constructs encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine tags.
33529230	2	48	theme	mammalian	375:383	arg1	coronaviruses					385:397	other mammalian coronaviruses	369:397	other mammalian coronaviruses	369:397	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	11	49	theme	mass	1891:1894	arg1	spectrometry					1896:1907	mass spectrometry	1891:1907	mass spectrometry	1891:1907	Deglycosylation with PNGase F and mass spectrometry confirmed the presence of N-glycosylation.
33529230	2	50	theme	unique	413:418	arg1	inducer					474:480	a significant inducer	460:480	a significant inducer of host immune response	460:504	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	2	50	theme	unique	413:418	arg1	RBD					445:447	RBD	445:447	RBD	445:447	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	2	50	theme	unique	413:418	arg1	domain					437:442	a unique receptor-binding domain	411:442	a unique receptor-binding domain (RBD)	411:448	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	8	51	theme	clonal	1463:1468	arg1	line					1484:1487	a high-yield clonal producer cell line	1450:1487	a high-yield clonal producer cell line	1450:1487	For the RBDv2 variant, a high-yield clonal producer cell line was obtained.
33529230	9	52	theme	RBD	1586:1588	arg1	protein					1590:1596	RBD protein	1586:1596	RBD protein	1586:1596	We developed a simple purification scheme that consistently yielded up to 30 mg of RBD protein per liter of the simple shake flask cell culture.
33529230	2	53	theme	S	355:355	arg1	proteins					357:364	the S proteins	351:364	the S proteins of other mammalian coronaviruses	351:397	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	5	54	from	hamster	1056:1062	arg1	sequences					963:971	the regulatory sequences	948:971	the regulatory sequences of the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1) from Chinese hamster	948:1062	Based on the previously developed p1.1 vector, containing the regulatory sequences of the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1) from Chinese hamster, we created two expression constructs encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine tags.
33529230	0	55	theme	monomeric	29:37	arg1	SARS-CoV-2					39:48	the monomeric SARS-CoV-2	25:48	the monomeric SARS-CoV-2	25:48	High-level expression of the monomeric SARS-CoV-2 S protein RBD 320-537 in stably transfected CHO cells by the EEF1A1-based plasmid vector.
33529230	8	56	theme	cell	1479:1482	arg1	line					1484:1487	a high-yield clonal producer cell line	1450:1487	a high-yield clonal producer cell line	1450:1487	For the RBDv2 variant, a high-yield clonal producer cell line was obtained.
33529230	3	57	theme	specific	561:568	arg1	RBD					530:532	Recombinant SARS-CoV-2 RBD	507:532	Recombinant SARS-CoV-2 RBD	507:532	Recombinant SARS-CoV-2 RBD is widely used as a highly specific minimal antigen for serological tests.
33529230	3	57	theme	specific	561:568	arg1	antigen					578:584	a highly specific minimal antigen	552:584	a highly specific minimal antigen for serological tests	552:606	Recombinant SARS-CoV-2 RBD is widely used as a highly specific minimal antigen for serological tests.
33529230	5	58	theme	regulatory	952:961	arg1	sequences					963:971	the regulatory sequences	948:971	the regulatory sequences of the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1) from Chinese hamster	948:1062	Based on the previously developed p1.1 vector, containing the regulatory sequences of the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1) from Chinese hamster, we created two expression constructs encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine tags.
33529230	2	59	theme	Coronavirus	289:299	arg1	protein					240:246	The S protein	234:246	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)	234:314	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	1	60	theme	proteins	182:189	arg1	protein					154:160	The spike (S) protein	140:160	The spike (S) protein	140:160	The spike (S) protein is one of the three proteins forming the coronaviruses' viral envelope.
33529230	1	60	theme	proteins	182:189	arg1	proteins					182:189	the three proteins	172:189	the three proteins forming the coronaviruses' viral envelope	172:231	The spike (S) protein is one of the three proteins forming the coronaviruses' viral envelope.
33529230	1	60	theme	proteins	182:189	arg1	one					165:167	one	165:167	one	165:167	The spike (S) protein is one of the three proteins forming the coronaviruses' viral envelope.
33529230	5	61	theme	developed	914:922	arg1	vector					929:934	the previously developed p1.1 vector	899:934	the previously developed p1.1 vector	899:934	Based on the previously developed p1.1 vector, containing the regulatory sequences of the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1) from Chinese hamster, we created two expression constructs encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine tags.
33529230	5	62	contain	containing	937:946	arg2	sequences					963:971	the regulatory sequences	948:971	the regulatory sequences of the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1) from Chinese hamster	948:1062	Based on the previously developed p1.1 vector, containing the regulatory sequences of the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1) from Chinese hamster, we created two expression constructs encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine tags.
33529230	5	62	contain	containing	937:946	arg1	vector					929:934	the previously developed p1.1 vector	899:934	the previously developed p1.1 vector	899:934	Based on the previously developed p1.1 vector, containing the regulatory sequences of the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1) from Chinese hamster, we created two expression constructs encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine tags.
33529230	3	63	theme	serological	590:600	arg1	tests					602:606	serological tests	590:606	serological tests	590:606	Recombinant SARS-CoV-2 RBD is widely used as a highly specific minimal antigen for serological tests.
33529230	7	64	from	genome	1419:1424	arg1	rounds					1344:1349	a few rounds	1338:1349	a few rounds of methotrexate-driven amplification of the genetic cassette in the genome	1338:1424	We transfected a CHO DG44 cell line, selected stably transfected cells, and performed a few rounds of methotrexate-driven amplification of the genetic cassette in the genome.
33529230	7	65	theme	cassette	1403:1410	arg1	amplification					1374:1386	methotrexate-driven amplification	1354:1386	methotrexate-driven amplification of the genetic cassette in the genome	1354:1424	We transfected a CHO DG44 cell line, selected stably transfected cells, and performed a few rounds of methotrexate-driven amplification of the genetic cassette in the genome.
33529230	10	66	theme	form	1811:1814	arg1	content					1816:1822	the monomeric form content	1797:1822	the monomeric form content	1797:1822	Purified proteins were analyzed by polyacrylamide gel electrophoresis in reducing and non-reducing conditions and gel filtration; for RBDv2 protein, the monomeric form content exceeded 90% for several series.
33529230	6	67	theme	signal	1203:1208	arg1	peptide					1210:1216	a native viral signal peptide	1188:1216	a native viral signal peptide	1188:1216	RBDv1 contained a native viral signal peptide, RBDv2 -human tPA signal peptide.
33529230	6	67	theme	signal	1203:1208	arg1	peptide					1243:1249	RBDv2 -human tPA signal peptide	1219:1249	RBDv2 -human tPA signal peptide	1219:1249	RBDv1 contained a native viral signal peptide, RBDv2 -human tPA signal peptide.
33529230	10	68	theme	Purified	1648:1655	arg1	proteins					1657:1664	Purified proteins	1648:1664	Purified proteins	1648:1664	Purified proteins were analyzed by polyacrylamide gel electrophoresis in reducing and non-reducing conditions and gel filtration; for RBDv2 protein, the monomeric form content exceeded 90% for several series.
33529230	5	69	theme	C-terminal	1131:1140	arg1	tags					1166:1169	C-terminal c-myc and polyhistidine tags	1131:1169	C-terminal c-myc and polyhistidine tags	1131:1169	Based on the previously developed p1.1 vector, containing the regulatory sequences of the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1) from Chinese hamster, we created two expression constructs encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine tags.
33529230	11	70	theme	N-glycosylation	1935:1949	arg1	presence					1923:1930	the presence	1919:1930	the presence of N-glycosylation	1919:1949	Deglycosylation with PNGase F and mass spectrometry confirmed the presence of N-glycosylation.
33529230	7	71	theme	amplification	1374:1386	arg1	rounds					1344:1349	a few rounds	1338:1349	a few rounds of methotrexate-driven amplification of the genetic cassette in the genome	1338:1424	We transfected a CHO DG44 cell line, selected stably transfected cells, and performed a few rounds of methotrexate-driven amplification of the genetic cassette in the genome.
33529230	6	72	theme	native	1190:1195	arg1	peptide					1210:1216	a native viral signal peptide	1188:1216	a native viral signal peptide	1188:1216	RBDv1 contained a native viral signal peptide, RBDv2 -human tPA signal peptide.
33529230	6	72	theme	native	1190:1195	arg1	peptide					1243:1249	RBDv2 -human tPA signal peptide	1219:1249	RBDv2 -human tPA signal peptide	1219:1249	RBDv1 contained a native viral signal peptide, RBDv2 -human tPA signal peptide.
33529230	2	73	theme	S	238:238	arg1	protein					240:246	The S protein	234:246	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)	234:314	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	4	74	contain	has	652:654	arg2	impact					670:675	a significant impact	656:675	a significant impact	656:675	Correct exposure of antigenic determinants has a significant impact on the accuracy of such tests-the antigen has to be correctly folded, contain no potentially antigenic non-vertebrate glycans, and, preferably, should have a glycosylation pattern similar to the native S protein.
33529230	4	74	contain	has	652:654	arg1	exposure					617:624	Correct exposure	609:624	Correct exposure of antigenic determinants	609:650	Correct exposure of antigenic determinants has a significant impact on the accuracy of such tests-the antigen has to be correctly folded, contain no potentially antigenic non-vertebrate glycans, and, preferably, should have a glycosylation pattern similar to the native S protein.
33529230	2	75	theme	Respiratory	268:278	arg1	Coronavirus					289:299	the Severe Acute Respiratory Syndrome Coronavirus 2	251:301	the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)	251:314	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	2	75	theme	Respiratory	268:278	arg1	SARS-CoV-2					304:313	SARS-CoV-2	304:313	SARS-CoV-2	304:313	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	4	76	theme	non-vertebrate	780:793	arg1	glycans					795:801	no potentially antigenic non-vertebrate glycans	755:801	no potentially antigenic non-vertebrate glycans	755:801	Correct exposure of antigenic determinants has a significant impact on the accuracy of such tests-the antigen has to be correctly folded, contain no potentially antigenic non-vertebrate glycans, and, preferably, should have a glycosylation pattern similar to the native S protein.
33529230	12	77	theme	vaccine	2046:2052	arg1	studies					2054:2060	subunit vaccine studies	2038:2060	subunit vaccine studies	2038:2060	The antigen produced by the described technique is suitable for serological tests and subunit vaccine studies.
33529230	0	78	theme	EEF1A1-based	111:122	arg1	vector					132:137	the EEF1A1-based plasmid vector	107:137	the EEF1A1-based plasmid vector	107:137	High-level expression of the monomeric SARS-CoV-2 S protein RBD 320-537 in stably transfected CHO cells by the EEF1A1-based plasmid vector.
33529230	2	79	theme	Severe	255:260	arg1	Coronavirus					289:299	the Severe Acute Respiratory Syndrome Coronavirus 2	251:301	the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)	251:314	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	2	79	theme	Severe	255:260	arg1	SARS-CoV-2					304:313	SARS-CoV-2	304:313	SARS-CoV-2	304:313	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	4	80	theme	glycosylation	835:847	arg1	pattern					849:855	a glycosylation pattern	833:855	a glycosylation pattern similar to the native S protein	833:887	Correct exposure of antigenic determinants has a significant impact on the accuracy of such tests-the antigen has to be correctly folded, contain no potentially antigenic non-vertebrate glycans, and, preferably, should have a glycosylation pattern similar to the native S protein.
33529230	10	81	theme	several	1841:1847	arg1	series					1849:1854	several series	1841:1854	several series	1841:1854	Purified proteins were analyzed by polyacrylamide gel electrophoresis in reducing and non-reducing conditions and gel filtration; for RBDv2 protein, the monomeric form content exceeded 90% for several series.
33529230	5	82	theme	elongation	1003:1012	arg1	factor					1014:1019	Eukaryotic translation elongation factor 1	980:1021	the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1)	976:1041	Based on the previously developed p1.1 vector, containing the regulatory sequences of the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1) from Chinese hamster, we created two expression constructs encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine tags.
33529230	4	83	theme	similar	857:863	arg1	pattern					849:855	a glycosylation pattern	833:855	a glycosylation pattern similar to the native S protein	833:887	Correct exposure of antigenic determinants has a significant impact on the accuracy of such tests-the antigen has to be correctly folded, contain no potentially antigenic non-vertebrate glycans, and, preferably, should have a glycosylation pattern similar to the native S protein.
33529230	0	84	from	320-537	64:70	arg1	cells					98:102	stably transfected CHO cells	75:102	stably transfected CHO cells	75:102	High-level expression of the monomeric SARS-CoV-2 S protein RBD 320-537 in stably transfected CHO cells by the EEF1A1-based plasmid vector.
33529230	12	85	theme	serological	2016:2026	arg1	tests					2028:2032	serological tests	2016:2032	serological tests	2016:2032	The antigen produced by the described technique is suitable for serological tests and subunit vaccine studies.
33529230	6	86	theme	signal	1236:1241	arg1	peptide					1210:1216	a native viral signal peptide	1188:1216	a native viral signal peptide	1188:1216	RBDv1 contained a native viral signal peptide, RBDv2 -human tPA signal peptide.
33529230	6	86	theme	signal	1236:1241	arg1	peptide					1243:1249	RBDv2 -human tPA signal peptide	1219:1249	RBDv2 -human tPA signal peptide	1219:1249	RBDv1 contained a native viral signal peptide, RBDv2 -human tPA signal peptide.
33529230	3	87	theme	Recombinant	507:517	arg1	RBD					530:532	Recombinant SARS-CoV-2 RBD	507:532	Recombinant SARS-CoV-2 RBD	507:532	Recombinant SARS-CoV-2 RBD is widely used as a highly specific minimal antigen for serological tests.
33529230	3	87	theme	Recombinant	507:517	arg1	antigen					578:584	a highly specific minimal antigen	552:584	a highly specific minimal antigen for serological tests	552:606	Recombinant SARS-CoV-2 RBD is widely used as a highly specific minimal antigen for serological tests.
33529230	2	88	theme	response	497:504	arg1	inducer					474:480	a significant inducer	460:480	a significant inducer of host immune response	460:504	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	2	88	theme	response	497:504	arg1	domain					437:442	a unique receptor-binding domain	411:442	a unique receptor-binding domain (RBD)	411:448	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	4	89	theme	determinants	639:650	arg1	exposure					617:624	Correct exposure	609:624	Correct exposure of antigenic determinants	609:650	Correct exposure of antigenic determinants has a significant impact on the accuracy of such tests-the antigen has to be correctly folded, contain no potentially antigenic non-vertebrate glycans, and, preferably, should have a glycosylation pattern similar to the native S protein.
33529230	6	90	theme	-human	1225:1230	arg1	peptide					1210:1216	a native viral signal peptide	1188:1216	a native viral signal peptide	1188:1216	RBDv1 contained a native viral signal peptide, RBDv2 -human tPA signal peptide.
33529230	6	90	theme	-human	1225:1230	arg1	peptide					1243:1249	RBDv2 -human tPA signal peptide	1219:1249	RBDv2 -human tPA signal peptide	1219:1249	RBDv1 contained a native viral signal peptide, RBDv2 -human tPA signal peptide.
33529230	2	91	theme	host	485:488	arg1	response					497:504	host immune response	485:504	host immune response	485:504	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	7	92	theme	cell	1278:1281	arg1	line					1283:1286	a CHO DG44 cell line	1267:1286	a CHO DG44 cell line	1267:1286	We transfected a CHO DG44 cell line, selected stably transfected cells, and performed a few rounds of methotrexate-driven amplification of the genetic cassette in the genome.
33529230	5	93	theme	Eukaryotic	980:989	arg1	factor					1014:1019	Eukaryotic translation elongation factor 1	980:1021	the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1)	976:1041	Based on the previously developed p1.1 vector, containing the regulatory sequences of the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1) from Chinese hamster, we created two expression constructs encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine tags.
33529230	4	94	dep	antigen	711:717	arg1	has					719:721	has	719:721	has to be correctly folded	719:744	Correct exposure of antigenic determinants has a significant impact on the accuracy of such tests-the antigen has to be correctly folded, contain no potentially antigenic non-vertebrate glycans, and, preferably, should have a glycosylation pattern similar to the native S protein.
33529230	9	95	theme	culture	1639:1645	arg1	liter					1602:1606	liter	1602:1606	liter of the simple shake flask cell culture	1602:1645	We developed a simple purification scheme that consistently yielded up to 30 mg of RBD protein per liter of the simple shake flask cell culture.
33529230	7	96	theme	CHO	1269:1271	arg1	line					1283:1286	a CHO DG44 cell line	1267:1286	a CHO DG44 cell line	1267:1286	We transfected a CHO DG44 cell line, selected stably transfected cells, and performed a few rounds of methotrexate-driven amplification of the genetic cassette in the genome.
33529230	4	97	theme	Correct	609:615	arg1	exposure					617:624	Correct exposure	609:624	Correct exposure of antigenic determinants	609:650	Correct exposure of antigenic determinants has a significant impact on the accuracy of such tests-the antigen has to be correctly folded, contain no potentially antigenic non-vertebrate glycans, and, preferably, should have a glycosylation pattern similar to the native S protein.
33529230	1	98	theme	spike	144:148	arg1	protein					154:160	The spike (S) protein	140:160	The spike (S) protein	140:160	The spike (S) protein is one of the three proteins forming the coronaviruses' viral envelope.
33529230	1	98	theme	spike	144:148	arg1	proteins					182:189	the three proteins	172:189	the three proteins forming the coronaviruses' viral envelope	172:231	The spike (S) protein is one of the three proteins forming the coronaviruses' viral envelope.
33529230	1	98	theme	spike	144:148	arg1	one					165:167	one	165:167	one	165:167	The spike (S) protein is one of the three proteins forming the coronaviruses' viral envelope.
33529230	2	99	theme	receptor-binding	420:435	arg1	inducer					474:480	a significant inducer	460:480	a significant inducer of host immune response	460:504	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	2	99	theme	receptor-binding	420:435	arg1	RBD					445:447	RBD	445:447	RBD	445:447	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	2	99	theme	receptor-binding	420:435	arg1	domain					437:442	a unique receptor-binding domain	411:442	a unique receptor-binding domain (RBD)	411:448	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	0	100	theme	CHO	94:96	arg1	cells					98:102	stably transfected CHO cells	75:102	stably transfected CHO cells	75:102	High-level expression of the monomeric SARS-CoV-2 S protein RBD 320-537 in stably transfected CHO cells by the EEF1A1-based plasmid vector.
33529230	9	101	theme	purification	1525:1536	arg1	scheme					1538:1543	a simple purification scheme	1516:1543	a simple purification scheme that consistently yielded up to 30 mg of RBD protein per liter of the simple shake flask cell culture	1516:1645	We developed a simple purification scheme that consistently yielded up to 30 mg of RBD protein per liter of the simple shake flask cell culture.
33529230	7	102	from	rounds	1344:1349	arg1	genome					1419:1424	the genome	1415:1424	the genome	1415:1424	We transfected a CHO DG44 cell line, selected stably transfected cells, and performed a few rounds of methotrexate-driven amplification of the genetic cassette in the genome.
33529230	8	103	theme	RBDv2	1435:1439	arg1	variant					1441:1447	the RBDv2 variant	1431:1447	the RBDv2 variant	1431:1447	For the RBDv2 variant, a high-yield clonal producer cell line was obtained.
33529230	5	104	theme	gene	1029:1032	arg1	sequences					963:971	the regulatory sequences	948:971	the regulatory sequences of the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1) from Chinese hamster	948:1062	Based on the previously developed p1.1 vector, containing the regulatory sequences of the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1) from Chinese hamster, we created two expression constructs encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine tags.
33529230	4	105	theme	S	879:879	arg1	protein					881:887	the native S protein	868:887	the native S protein	868:887	Correct exposure of antigenic determinants has a significant impact on the accuracy of such tests-the antigen has to be correctly folded, contain no potentially antigenic non-vertebrate glycans, and, preferably, should have a glycosylation pattern similar to the native S protein.
33529230	4	106	theme	tests-the	701:709	arg1	antigen					711:717	such tests-the antigen	696:717	such tests-the antigen has to be correctly folded	696:744	Correct exposure of antigenic determinants has a significant impact on the accuracy of such tests-the antigen has to be correctly folded, contain no potentially antigenic non-vertebrate glycans, and, preferably, should have a glycosylation pattern similar to the native S protein.
33529230	2	107	theme	similar	340:346	arg1	structure					330:338	a spatial structure	320:338	a spatial structure similar to the S proteins of other mammalian coronaviruses	320:397	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	2	108	theme	coronaviruses	385:397	arg1	proteins					357:364	the S proteins	351:364	the S proteins of other mammalian coronaviruses	351:397	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	8	109	theme	high-yield	1452:1461	arg1	line					1484:1487	a high-yield clonal producer cell line	1450:1487	a high-yield clonal producer cell line	1450:1487	For the RBDv2 variant, a high-yield clonal producer cell line was obtained.
33529230	2	110	theme	spatial	322:328	arg1	structure					330:338	a spatial structure	320:338	a spatial structure similar to the S proteins of other mammalian coronaviruses	320:397	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	6	111	contain	contained	1178:1186	arg1	RBDv1					1172:1176	RBDv1	1172:1176	RBDv1	1172:1176	RBDv1 contained a native viral signal peptide, RBDv2 -human tPA signal peptide.
33529230	6	111	contain	contained	1178:1186	arg2	peptide					1210:1216	a native viral signal peptide	1188:1216	a native viral signal peptide	1188:1216	RBDv1 contained a native viral signal peptide, RBDv2 -human tPA signal peptide.
33529230	6	111	contain	contained	1178:1186	arg2	peptide					1243:1249	RBDv2 -human tPA signal peptide	1219:1249	RBDv2 -human tPA signal peptide	1219:1249	RBDv1 contained a native viral signal peptide, RBDv2 -human tPA signal peptide.
33529230	2	112	theme	other	369:373	arg1	coronaviruses					385:397	other mammalian coronaviruses	369:397	other mammalian coronaviruses	369:397	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	8	113	theme	producer	1470:1477	arg1	line					1484:1487	a high-yield clonal producer cell line	1450:1487	a high-yield clonal producer cell line	1450:1487	For the RBDv2 variant, a high-yield clonal producer cell line was obtained.
33529230	3	114	theme	minimal	570:576	arg1	RBD					530:532	Recombinant SARS-CoV-2 RBD	507:532	Recombinant SARS-CoV-2 RBD	507:532	Recombinant SARS-CoV-2 RBD is widely used as a highly specific minimal antigen for serological tests.
33529230	3	114	theme	minimal	570:576	arg1	antigen					578:584	a highly specific minimal antigen	552:584	a highly specific minimal antigen for serological tests	552:606	Recombinant SARS-CoV-2 RBD is widely used as a highly specific minimal antigen for serological tests.
33529230	0	115	theme	SARS-CoV-2	39:48	arg1	expression					11:20	High-level expression	0:20	High-level expression of the monomeric SARS-CoV-2	0:48	High-level expression of the monomeric SARS-CoV-2 S protein RBD 320-537 in stably transfected CHO cells by the EEF1A1-based plasmid vector.
33529230	5	116	theme	SARS-CoV-2	1111:1120	arg1	RBD					1122:1124	SARS-CoV-2 RBD	1111:1124	SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine tags	1111:1169	Based on the previously developed p1.1 vector, containing the regulatory sequences of the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1) from Chinese hamster, we created two expression constructs encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine tags.
33529230	10	117	theme	gel	1762:1764	arg1	filtration					1766:1775	gel filtration	1762:1775	gel filtration	1762:1775	Purified proteins were analyzed by polyacrylamide gel electrophoresis in reducing and non-reducing conditions and gel filtration; for RBDv2 protein, the monomeric form content exceeded 90% for several series.
33529230	0	118	theme	protein	52:58	arg1	320-537					64:70	protein RBD 320-537	52:70	protein RBD 320-537 in stably transfected CHO cells	52:102	High-level expression of the monomeric SARS-CoV-2 S protein RBD 320-537 in stably transfected CHO cells by the EEF1A1-based plasmid vector.
33529230	5	119	with	RBD	1122:1124	arg1	tags					1166:1169	C-terminal c-myc and polyhistidine tags	1131:1169	C-terminal c-myc and polyhistidine tags	1131:1169	Based on the previously developed p1.1 vector, containing the regulatory sequences of the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1) from Chinese hamster, we created two expression constructs encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine tags.
33529230	9	120	theme	simple	1518:1523	arg1	scheme					1538:1543	a simple purification scheme	1516:1543	a simple purification scheme that consistently yielded up to 30 mg of RBD protein per liter of the simple shake flask cell culture	1516:1645	We developed a simple purification scheme that consistently yielded up to 30 mg of RBD protein per liter of the simple shake flask cell culture.
33529230	10	121	theme	RBDv2	1782:1786	arg1	protein					1788:1794	RBDv2 protein	1782:1794	RBDv2 protein	1782:1794	Purified proteins were analyzed by polyacrylamide gel electrophoresis in reducing and non-reducing conditions and gel filtration; for RBDv2 protein, the monomeric form content exceeded 90% for several series.
33529230	2	122	theme	Syndrome	280:287	arg1	Coronavirus					289:299	the Severe Acute Respiratory Syndrome Coronavirus 2	251:301	the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)	251:314	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	2	122	theme	Syndrome	280:287	arg1	SARS-CoV-2					304:313	SARS-CoV-2	304:313	SARS-CoV-2	304:313	The S protein of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has a spatial structure similar to the S proteins of other mammalian coronaviruses, except for a unique receptor-binding domain (RBD), which is a significant inducer of host immune response.
33529230	5	123	theme	polyhistidine	1152:1164	arg1	tags					1166:1169	C-terminal c-myc and polyhistidine tags	1131:1169	C-terminal c-myc and polyhistidine tags	1131:1169	Based on the previously developed p1.1 vector, containing the regulatory sequences of the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1) from Chinese hamster, we created two expression constructs encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine tags.
33529230	5	124	theme	p1.1	924:927	arg1	vector					929:934	the previously developed p1.1 vector	899:934	the previously developed p1.1 vector	899:934	Based on the previously developed p1.1 vector, containing the regulatory sequences of the Eukaryotic translation elongation factor 1 alpha gene (EEF1A1) from Chinese hamster, we created two expression constructs encoding SARS-CoV-2 RBD with C-terminal c-myc and polyhistidine tags.
33529230	9	125	dep	30	1577:1578	arg1	to					1574:1575	to	1574:1575	to	1574:1575	We developed a simple purification scheme that consistently yielded up to 30 mg of RBD protein per liter of the simple shake flask cell culture.
32008579	6	0	theme	adipocyte	1252:1260	arg1	expression					1269:1278	fasting-induced adipocyte factor expression	1236:1278	fasting-induced adipocyte factor expression leading to dyslipidaemia	1236:1303	Reduced amounts of beneficial micro-organisms also inhibit fasting-induced adipocyte factor expression leading to dyslipidaemia.
32008579	9	1	theme	obesogenic	1613:1622	arg1	pathways					1636:1643	these obesogenic mechanistic pathways	1607:1643	these obesogenic mechanistic pathways that are transmittable from one generation to another	1607:1697	Interestingly, these obesogenic mechanistic pathways that are transmittable from one generation to another can be modulated through the administration of probiotics, prebiotics and synbiotics.
32008579	3	2	dep	eubiotic	534:541	arg1	health-promoting					544:559	health-promoting	544:559	health-promoting	544:559	We also discussed the eubiotic, health-promoting effects of probiotics and prebiotics as well as epigenetic modifications associated with gut microbial dysbiosis and risk of obesity.
32008579	3	3	theme	prebiotics	587:596	arg1	modifications					620:632	epigenetic modifications	609:632	the eubiotic, health-promoting effects of probiotics and prebiotics as well as epigenetic modifications associated with gut microbial dysbiosis and risk of obesity	530:692	We also discussed the eubiotic, health-promoting effects of probiotics and prebiotics as well as epigenetic modifications associated with gut microbial dysbiosis and risk of obesity.
32008579	3	3	theme	prebiotics	587:596	arg1	effects					561:567	the eubiotic, health-promoting effects	530:567	the eubiotic, health-promoting effects of probiotics and prebiotics as well as epigenetic modifications associated with gut microbial dysbiosis and risk of obesity	530:692	We also discussed the eubiotic, health-promoting effects of probiotics and prebiotics as well as epigenetic modifications associated with gut microbial dysbiosis and risk of obesity.
32008579	1	4	theme	metabolic	272:280	arg1	products					282:289	their metabolic products	266:289	their metabolic products	266:289	The global obesity epidemic has necessitated the search for better intervention strategies including the exploitation of the health benefits of some gut microbiota and their metabolic products.
32008579	3	5	theme	probiotics	572:581	arg1	modifications					620:632	epigenetic modifications	609:632	the eubiotic, health-promoting effects of probiotics and prebiotics as well as epigenetic modifications associated with gut microbial dysbiosis and risk of obesity	530:692	We also discussed the eubiotic, health-promoting effects of probiotics and prebiotics as well as epigenetic modifications associated with gut microbial dysbiosis and risk of obesity.
32008579	3	5	theme	probiotics	572:581	arg1	effects					561:567	the eubiotic, health-promoting effects	530:567	the eubiotic, health-promoting effects of probiotics and prebiotics as well as epigenetic modifications associated with gut microbial dysbiosis and risk of obesity	530:692	We also discussed the eubiotic, health-promoting effects of probiotics and prebiotics as well as epigenetic modifications associated with gut microbial dysbiosis and risk of obesity.
32008579	5	6	theme	gut	955:957	arg1	microbiota					959:968	Altered gut microbiota	947:968	Altered gut microbiota	947:968	Altered gut microbiota is associated with decreased expression of SCFA that maintain intestinal epithelial barrier integrity, reduce bacterial translocation and inflammation and increase expression of hunger-suppressing hormones.
32008579	10	7	from	application	1936:1946	arg1	humans					1951:1956	humans	1951:1956	humans	1951:1956	Though the influence of gut microbiota on the risk of obesity and several intervention strategies have been extensively demonstrated in animal models, application in humans still requires further robust investigation.
32008579	4	8	theme	gut	737:739	arg1	microbiota					741:750	the gut microbiota	733:750	the gut microbiota to one predominated by Firmicutes (Clostridium), Prevotella and Methanobrevibacter but deficient in beneficial genera/species such as Bacteroides, Bifidobacterium, Lactobacillus and Akkermansia	733:944	High-fat/carbohydrate diet programmes the gut microbiota to one predominated by Firmicutes (Clostridium), Prevotella and Methanobrevibacter but deficient in beneficial genera/species such as Bacteroides, Bifidobacterium, Lactobacillus and Akkermansia.
32008579	10	9	theme	microbiota	1813:1822	arg1	influence					1796:1804	the influence	1792:1804	the influence of gut microbiota on the risk of obesity and several intervention strategies	1792:1881	Though the influence of gut microbiota on the risk of obesity and several intervention strategies have been extensively demonstrated in animal models, application in humans still requires further robust investigation.
32008579	3	10	theme	epigenetic	609:618	arg1	modifications					620:632	epigenetic modifications	609:632	the eubiotic, health-promoting effects of probiotics and prebiotics as well as epigenetic modifications associated with gut microbial dysbiosis and risk of obesity	530:692	We also discussed the eubiotic, health-promoting effects of probiotics and prebiotics as well as epigenetic modifications associated with gut microbial dysbiosis and risk of obesity.
32008579	8	11	theme	diet	1447:1450	arg1	synergy					1427:1433	The synergy	1423:1433	The synergy of high-fat diet and dysbiotic gut microbiota	1423:1479	The synergy of high-fat diet and dysbiotic gut microbiota initiates a recipe that epigenetically programmes the host for increased adiposity and poor glycaemic control.
32008579	1	12	theme	epidemic	117:124	arg1	obesity					109:115	The global obesity epidemic	98:124	The global obesity epidemic	98:124	The global obesity epidemic has necessitated the search for better intervention strategies including the exploitation of the health benefits of some gut microbiota and their metabolic products.
32008579	4	13	theme	High-fat/carbohydrate	695:715	arg1	diet					717:720	High-fat/carbohydrate diet	695:720	High-fat/carbohydrate diet	695:720	High-fat/carbohydrate diet programmes the gut microbiota to one predominated by Firmicutes (Clostridium), Prevotella and Methanobrevibacter but deficient in beneficial genera/species such as Bacteroides, Bifidobacterium, Lactobacillus and Akkermansia.
32008579	3	14	theme	gut	650:652	arg1	dysbiosis					664:672	gut microbial dysbiosis	650:672	gut microbial dysbiosis	650:672	We also discussed the eubiotic, health-promoting effects of probiotics and prebiotics as well as epigenetic modifications associated with gut microbial dysbiosis and risk of obesity.
32008579	1	15	theme	health	223:228	arg1	benefits					230:237	the health benefits	219:237	the health benefits of some gut microbiota	219:260	The global obesity epidemic has necessitated the search for better intervention strategies including the exploitation of the health benefits of some gut microbiota and their metabolic products.
32008579	8	16	theme	microbiota	1470:1479	arg1	synergy					1427:1433	The synergy	1423:1433	The synergy of high-fat diet and dysbiotic gut microbiota	1423:1479	The synergy of high-fat diet and dysbiotic gut microbiota initiates a recipe that epigenetically programmes the host for increased adiposity and poor glycaemic control.
32008579	5	17	theme	SCFA	1013:1016	arg1	expression					999:1008	decreased expression	989:1008	decreased expression of SCFA that maintain intestinal epithelial barrier integrity, reduce bacterial translocation and inflammation and increase expression of hunger-suppressing hormones	989:1174	Altered gut microbiota is associated with decreased expression of SCFA that maintain intestinal epithelial barrier integrity, reduce bacterial translocation and inflammation and increase expression of hunger-suppressing hormones.
32008579	4	18	from	genera/species	863:876	arg1	deficient					839:847	deficient	839:847	deficient	839:847	High-fat/carbohydrate diet programmes the gut microbiota to one predominated by Firmicutes (Clostridium), Prevotella and Methanobrevibacter but deficient in beneficial genera/species such as Bacteroides, Bifidobacterium, Lactobacillus and Akkermansia.
32008579	1	19	theme	better	158:163	arg1	products					282:289	their metabolic products	266:289	their metabolic products	266:289	The global obesity epidemic has necessitated the search for better intervention strategies including the exploitation of the health benefits of some gut microbiota and their metabolic products.
32008579	1	19	theme	better	158:163	arg1	strategies					178:187	better intervention strategies	158:187	better intervention strategies including the exploitation of the health benefits of some gut microbiota and their metabolic products	158:289	The global obesity epidemic has necessitated the search for better intervention strategies including the exploitation of the health benefits of some gut microbiota and their metabolic products.
32008579	1	19	theme	better	158:163	arg1	exploitation					203:214	the exploitation	199:214	the exploitation of the health benefits of some gut microbiota	199:260	The global obesity epidemic has necessitated the search for better intervention strategies including the exploitation of the health benefits of some gut microbiota and their metabolic products.
32008579	0	20	theme	metabolism	86:95	arg1	homoeostasis					63:74	homoeostasis	63:74	homoeostasis of energy metabolism	63:95	Microbial dysbiosis-induced obesity: role of gut microbiota in homoeostasis of energy metabolism.
32008579	10	21	theme	robust	1981:1986	arg1	investigation					1988:2000	further robust investigation	1973:2000	further robust investigation	1973:2000	Though the influence of gut microbiota on the risk of obesity and several intervention strategies have been extensively demonstrated in animal models, application in humans still requires further robust investigation.
32008579	4	22	dep	one	755:757	arg1	predominated					759:770	predominated	759:770	predominated by Firmicutes (Clostridium), Prevotella and Methanobrevibacter	759:833	High-fat/carbohydrate diet programmes the gut microbiota to one predominated by Firmicutes (Clostridium), Prevotella and Methanobrevibacter but deficient in beneficial genera/species such as Bacteroides, Bifidobacterium, Lactobacillus and Akkermansia.
32008579	4	22	dep	one	755:757	arg1	deficient					839:847	deficient	839:847	deficient	839:847	High-fat/carbohydrate diet programmes the gut microbiota to one predominated by Firmicutes (Clostridium), Prevotella and Methanobrevibacter but deficient in beneficial genera/species such as Bacteroides, Bifidobacterium, Lactobacillus and Akkermansia.
32008579	8	23	theme	increased	1544:1552	arg1	adiposity					1554:1562	increased adiposity	1544:1562	increased adiposity	1544:1562	The synergy of high-fat diet and dysbiotic gut microbiota initiates a recipe that epigenetically programmes the host for increased adiposity and poor glycaemic control.
32008579	10	24	theme	strategies	1872:1881	arg1	risk					1831:1834	the risk	1827:1834	the risk of obesity and several intervention strategies	1827:1881	Though the influence of gut microbiota on the risk of obesity and several intervention strategies have been extensively demonstrated in animal models, application in humans still requires further robust investigation.
32008579	9	25	theme	synbiotics	1773:1782	arg1	administration					1728:1741	the administration	1724:1741	the administration of probiotics, prebiotics and synbiotics	1724:1782	Interestingly, these obesogenic mechanistic pathways that are transmittable from one generation to another can be modulated through the administration of probiotics, prebiotics and synbiotics.
32008579	2	26	theme	metabolic	476:484	arg1	inflammation					486:497	dysbiosis-induced metabolic inflammation	458:497	dysbiosis-induced metabolic inflammation	458:497	Therefore, we examined the gut microbial composition and mechanisms of interaction with the host in relation to homoeostatic energy metabolism and pathophysiology of dysbiosis-induced metabolic inflammation and obesity.
32008579	0	27	from	role	37:40	arg1	homoeostasis					63:74	homoeostasis	63:74	homoeostasis of energy metabolism	63:95	Microbial dysbiosis-induced obesity: role of gut microbiota in homoeostasis of energy metabolism.
32008579	2	28	dep	composition	333:343	arg1	the					315:317	the	315:317	the	315:317	Therefore, we examined the gut microbial composition and mechanisms of interaction with the host in relation to homoeostatic energy metabolism and pathophysiology of dysbiosis-induced metabolic inflammation and obesity.
32008579	10	29	theme	several	1851:1857	arg1	strategies					1872:1881	several intervention strategies	1851:1881	several intervention strategies	1851:1881	Though the influence of gut microbiota on the risk of obesity and several intervention strategies have been extensively demonstrated in animal models, application in humans still requires further robust investigation.
32008579	0	30	theme	Microbial	0:8	arg1	obesity					28:34	Microbial dysbiosis-induced obesity	0:34	Microbial dysbiosis-induced obesity	0:34	Microbial dysbiosis-induced obesity: role of gut microbiota in homoeostasis of energy metabolism.
32008579	5	31	theme	epithelial	1043:1052	arg1	integrity					1062:1070	intestinal epithelial barrier integrity	1032:1070	intestinal epithelial barrier integrity	1032:1070	Altered gut microbiota is associated with decreased expression of SCFA that maintain intestinal epithelial barrier integrity, reduce bacterial translocation and inflammation and increase expression of hunger-suppressing hormones.
32008579	6	32	theme	beneficial	1196:1205	arg1	micro-organisms					1207:1221	beneficial micro-organisms	1196:1221	beneficial micro-organisms	1196:1221	Reduced amounts of beneficial micro-organisms also inhibit fasting-induced adipocyte factor expression leading to dyslipidaemia.
32008579	8	33	theme	glycaemic	1573:1581	arg1	control					1583:1589	poor glycaemic control	1568:1589	poor glycaemic control	1568:1589	The synergy of high-fat diet and dysbiotic gut microbiota initiates a recipe that epigenetically programmes the host for increased adiposity and poor glycaemic control.
32008579	7	34	theme	metabolic	1340:1348	arg1	inflammation					1326:1337	A low-grade chronic inflammation	1306:1337	A low-grade chronic inflammation (metabolic endotoxaemia)	1306:1362	A low-grade chronic inflammation (metabolic endotoxaemia) ensues which culminates in obesity and its co-morbidities.
32008579	7	34	theme	metabolic	1340:1348	arg1	endotoxaemia					1350:1361	metabolic endotoxaemia	1340:1361	metabolic endotoxaemia	1340:1361	A low-grade chronic inflammation (metabolic endotoxaemia) ensues which culminates in obesity and its co-morbidities.
32008579	1	35	theme	global	102:107	arg1	obesity					109:115	The global obesity epidemic	98:124	The global obesity epidemic	98:124	The global obesity epidemic has necessitated the search for better intervention strategies including the exploitation of the health benefits of some gut microbiota and their metabolic products.
32008579	2	36	theme	energy	417:422	arg1	metabolism					424:433	homoeostatic energy metabolism	404:433	homoeostatic energy metabolism	404:433	Therefore, we examined the gut microbial composition and mechanisms of interaction with the host in relation to homoeostatic energy metabolism and pathophysiology of dysbiosis-induced metabolic inflammation and obesity.
32008579	5	37	theme	Altered	947:953	arg1	microbiota					959:968	Altered gut microbiota	947:968	Altered gut microbiota	947:968	Altered gut microbiota is associated with decreased expression of SCFA that maintain intestinal epithelial barrier integrity, reduce bacterial translocation and inflammation and increase expression of hunger-suppressing hormones.
32008579	3	38	theme	obesity	686:692	arg1	dysbiosis					664:672	gut microbial dysbiosis	650:672	gut microbial dysbiosis	650:672	We also discussed the eubiotic, health-promoting effects of probiotics and prebiotics as well as epigenetic modifications associated with gut microbial dysbiosis and risk of obesity.
32008579	3	38	theme	obesity	686:692	arg1	risk					678:681	risk	678:681	risk of obesity	678:692	We also discussed the eubiotic, health-promoting effects of probiotics and prebiotics as well as epigenetic modifications associated with gut microbial dysbiosis and risk of obesity.
32008579	0	39	theme	gut	45:47	arg1	microbiota					49:58	gut microbiota	45:58	gut microbiota	45:58	Microbial dysbiosis-induced obesity: role of gut microbiota in homoeostasis of energy metabolism.
32008579	2	40	with	interaction	363:373	arg1	host					384:387	the host	380:387	the host	380:387	Therefore, we examined the gut microbial composition and mechanisms of interaction with the host in relation to homoeostatic energy metabolism and pathophysiology of dysbiosis-induced metabolic inflammation and obesity.
32008579	5	41	theme	hormones	1167:1174	arg1	expression					1134:1143	expression	1134:1143	expression of hunger-suppressing hormones	1134:1174	Altered gut microbiota is associated with decreased expression of SCFA that maintain intestinal epithelial barrier integrity, reduce bacterial translocation and inflammation and increase expression of hunger-suppressing hormones.
32008579	10	42	theme	animal	1921:1926	arg1	models					1928:1933	animal models	1921:1933	animal models	1921:1933	Though the influence of gut microbiota on the risk of obesity and several intervention strategies have been extensively demonstrated in animal models, application in humans still requires further robust investigation.
32008579	1	43	theme	benefits	230:237	arg1	products					282:289	their metabolic products	266:289	their metabolic products	266:289	The global obesity epidemic has necessitated the search for better intervention strategies including the exploitation of the health benefits of some gut microbiota and their metabolic products.
32008579	1	43	theme	benefits	230:237	arg1	exploitation					203:214	the exploitation	199:214	the exploitation of the health benefits of some gut microbiota	199:260	The global obesity epidemic has necessitated the search for better intervention strategies including the exploitation of the health benefits of some gut microbiota and their metabolic products.
32008579	10	44	from	influence	1796:1804	arg1	risk					1831:1834	the risk	1827:1834	the risk of obesity and several intervention strategies	1827:1881	Though the influence of gut microbiota on the risk of obesity and several intervention strategies have been extensively demonstrated in animal models, application in humans still requires further robust investigation.
32008579	6	45	theme	factor	1262:1267	arg1	expression					1269:1278	fasting-induced adipocyte factor expression	1236:1278	fasting-induced adipocyte factor expression leading to dyslipidaemia	1236:1303	Reduced amounts of beneficial micro-organisms also inhibit fasting-induced adipocyte factor expression leading to dyslipidaemia.
32008579	2	46	theme	interaction	363:373	arg1	mechanisms					349:358	mechanisms	349:358	mechanisms	349:358	Therefore, we examined the gut microbial composition and mechanisms of interaction with the host in relation to homoeostatic energy metabolism and pathophysiology of dysbiosis-induced metabolic inflammation and obesity.
32008579	2	46	theme	interaction	363:373	arg1	composition					333:343	gut microbial composition	319:343	gut microbial composition	319:343	Therefore, we examined the gut microbial composition and mechanisms of interaction with the host in relation to homoeostatic energy metabolism and pathophysiology of dysbiosis-induced metabolic inflammation and obesity.
32008579	1	47	theme	microbiota	251:260	arg1	benefits					230:237	the health benefits	219:237	the health benefits of some gut microbiota	219:260	The global obesity epidemic has necessitated the search for better intervention strategies including the exploitation of the health benefits of some gut microbiota and their metabolic products.
32008579	2	48	from	composition	333:343	arg1	relation					392:399	relation	392:399	relation to homoeostatic energy metabolism and pathophysiology of dysbiosis-induced metabolic inflammation and obesity	392:509	Therefore, we examined the gut microbial composition and mechanisms of interaction with the host in relation to homoeostatic energy metabolism and pathophysiology of dysbiosis-induced metabolic inflammation and obesity.
32008579	6	49	theme	fasting-induced	1236:1250	arg1	expression					1269:1278	fasting-induced adipocyte factor expression	1236:1278	fasting-induced adipocyte factor expression leading to dyslipidaemia	1236:1303	Reduced amounts of beneficial micro-organisms also inhibit fasting-induced adipocyte factor expression leading to dyslipidaemia.
32008579	9	50	theme	mechanistic	1624:1634	arg1	pathways					1636:1643	these obesogenic mechanistic pathways	1607:1643	these obesogenic mechanistic pathways that are transmittable from one generation to another	1607:1697	Interestingly, these obesogenic mechanistic pathways that are transmittable from one generation to another can be modulated through the administration of probiotics, prebiotics and synbiotics.
32008579	2	51	from	mechanisms	349:358	arg1	relation					392:399	relation	392:399	relation to homoeostatic energy metabolism and pathophysiology of dysbiosis-induced metabolic inflammation and obesity	392:509	Therefore, we examined the gut microbial composition and mechanisms of interaction with the host in relation to homoeostatic energy metabolism and pathophysiology of dysbiosis-induced metabolic inflammation and obesity.
32008579	10	52	theme	further	1973:1979	arg1	investigation					1988:2000	further robust investigation	1973:2000	further robust investigation	1973:2000	Though the influence of gut microbiota on the risk of obesity and several intervention strategies have been extensively demonstrated in animal models, application in humans still requires further robust investigation.
32008579	1	53	theme	intervention	165:176	arg1	products					282:289	their metabolic products	266:289	their metabolic products	266:289	The global obesity epidemic has necessitated the search for better intervention strategies including the exploitation of the health benefits of some gut microbiota and their metabolic products.
32008579	1	53	theme	intervention	165:176	arg1	strategies					178:187	better intervention strategies	158:187	better intervention strategies including the exploitation of the health benefits of some gut microbiota and their metabolic products	158:289	The global obesity epidemic has necessitated the search for better intervention strategies including the exploitation of the health benefits of some gut microbiota and their metabolic products.
32008579	1	53	theme	intervention	165:176	arg1	exploitation					203:214	the exploitation	199:214	the exploitation of the health benefits of some gut microbiota	199:260	The global obesity epidemic has necessitated the search for better intervention strategies including the exploitation of the health benefits of some gut microbiota and their metabolic products.
32008579	7	54	theme	low-grade	1308:1316	arg1	inflammation					1326:1337	A low-grade chronic inflammation	1306:1337	A low-grade chronic inflammation (metabolic endotoxaemia)	1306:1362	A low-grade chronic inflammation (metabolic endotoxaemia) ensues which culminates in obesity and its co-morbidities.
32008579	7	54	theme	low-grade	1308:1316	arg1	endotoxaemia					1350:1361	metabolic endotoxaemia	1340:1361	metabolic endotoxaemia	1340:1361	A low-grade chronic inflammation (metabolic endotoxaemia) ensues which culminates in obesity and its co-morbidities.
32008579	2	55	theme	gut	319:321	arg1	composition					333:343	gut microbial composition	319:343	gut microbial composition	319:343	Therefore, we examined the gut microbial composition and mechanisms of interaction with the host in relation to homoeostatic energy metabolism and pathophysiology of dysbiosis-induced metabolic inflammation and obesity.
32008579	10	56	theme	gut	1809:1811	arg1	microbiota					1813:1822	gut microbiota	1809:1822	gut microbiota	1809:1822	Though the influence of gut microbiota on the risk of obesity and several intervention strategies have been extensively demonstrated in animal models, application in humans still requires further robust investigation.
32008579	0	57	dep	role	37:40	arg1	obesity					28:34	Microbial dysbiosis-induced obesity	0:34	Microbial dysbiosis-induced obesity	0:34	Microbial dysbiosis-induced obesity: role of gut microbiota in homoeostasis of energy metabolism.
32008579	9	58	theme	probiotics	1746:1755	arg1	administration					1728:1741	the administration	1724:1741	the administration of probiotics, prebiotics and synbiotics	1724:1782	Interestingly, these obesogenic mechanistic pathways that are transmittable from one generation to another can be modulated through the administration of probiotics, prebiotics and synbiotics.
32008579	8	59	theme	high-fat	1438:1445	arg1	diet					1447:1450	high-fat diet	1438:1450	high-fat diet	1438:1450	The synergy of high-fat diet and dysbiotic gut microbiota initiates a recipe that epigenetically programmes the host for increased adiposity and poor glycaemic control.
32008579	3	60	theme	eubiotic	534:541	arg1	effects					561:567	the eubiotic, health-promoting effects	530:567	the eubiotic, health-promoting effects of probiotics and prebiotics as well as epigenetic modifications associated with gut microbial dysbiosis and risk of obesity	530:692	We also discussed the eubiotic, health-promoting effects of probiotics and prebiotics as well as epigenetic modifications associated with gut microbial dysbiosis and risk of obesity.
32008579	8	61	theme	gut	1466:1468	arg1	microbiota					1470:1479	dysbiotic gut microbiota	1456:1479	dysbiotic gut microbiota	1456:1479	The synergy of high-fat diet and dysbiotic gut microbiota initiates a recipe that epigenetically programmes the host for increased adiposity and poor glycaemic control.
32008579	3	62	theme	microbial	654:662	arg1	dysbiosis					664:672	gut microbial dysbiosis	650:672	gut microbial dysbiosis	650:672	We also discussed the eubiotic, health-promoting effects of probiotics and prebiotics as well as epigenetic modifications associated with gut microbial dysbiosis and risk of obesity.
32008579	0	63	theme	energy	79:84	arg1	metabolism					86:95	energy metabolism	79:95	energy metabolism	79:95	Microbial dysbiosis-induced obesity: role of gut microbiota in homoeostasis of energy metabolism.
32008579	5	64	theme	decreased	989:997	arg1	expression					999:1008	decreased expression	989:1008	decreased expression of SCFA that maintain intestinal epithelial barrier integrity, reduce bacterial translocation and inflammation and increase expression of hunger-suppressing hormones	989:1174	Altered gut microbiota is associated with decreased expression of SCFA that maintain intestinal epithelial barrier integrity, reduce bacterial translocation and inflammation and increase expression of hunger-suppressing hormones.
32008579	4	65	from	deficient	839:847	arg1	Akkermansia					934:944	Akkermansia	934:944	Akkermansia	934:944	High-fat/carbohydrate diet programmes the gut microbiota to one predominated by Firmicutes (Clostridium), Prevotella and Methanobrevibacter but deficient in beneficial genera/species such as Bacteroides, Bifidobacterium, Lactobacillus and Akkermansia.
32008579	4	65	from	deficient	839:847	arg1	Lactobacillus					916:928	Lactobacillus	916:928	Lactobacillus	916:928	High-fat/carbohydrate diet programmes the gut microbiota to one predominated by Firmicutes (Clostridium), Prevotella and Methanobrevibacter but deficient in beneficial genera/species such as Bacteroides, Bifidobacterium, Lactobacillus and Akkermansia.
32008579	4	65	from	deficient	839:847	arg1	Bifidobacterium					899:913	Bifidobacterium	899:913	Bifidobacterium	899:913	High-fat/carbohydrate diet programmes the gut microbiota to one predominated by Firmicutes (Clostridium), Prevotella and Methanobrevibacter but deficient in beneficial genera/species such as Bacteroides, Bifidobacterium, Lactobacillus and Akkermansia.
32008579	4	65	from	deficient	839:847	arg1	genera/species					863:876	beneficial genera/species	852:876	beneficial genera/species such as Bacteroides, Bifidobacterium, Lactobacillus and Akkermansia	852:944	High-fat/carbohydrate diet programmes the gut microbiota to one predominated by Firmicutes (Clostridium), Prevotella and Methanobrevibacter but deficient in beneficial genera/species such as Bacteroides, Bifidobacterium, Lactobacillus and Akkermansia.
32008579	4	65	from	deficient	839:847	arg1	Bacteroides					886:896	Bacteroides	886:896	Bacteroides	886:896	High-fat/carbohydrate diet programmes the gut microbiota to one predominated by Firmicutes (Clostridium), Prevotella and Methanobrevibacter but deficient in beneficial genera/species such as Bacteroides, Bifidobacterium, Lactobacillus and Akkermansia.
32008579	9	66	theme	prebiotics	1758:1767	arg1	administration					1728:1741	the administration	1724:1741	the administration of probiotics, prebiotics and synbiotics	1724:1782	Interestingly, these obesogenic mechanistic pathways that are transmittable from one generation to another can be modulated through the administration of probiotics, prebiotics and synbiotics.
32008579	5	67	theme	bacterial	1080:1088	arg1	translocation					1090:1102	bacterial translocation	1080:1102	bacterial translocation	1080:1102	Altered gut microbiota is associated with decreased expression of SCFA that maintain intestinal epithelial barrier integrity, reduce bacterial translocation and inflammation and increase expression of hunger-suppressing hormones.
32008579	10	68	theme	obesity	1839:1845	arg1	risk					1831:1834	the risk	1827:1834	the risk of obesity and several intervention strategies	1827:1881	Though the influence of gut microbiota on the risk of obesity and several intervention strategies have been extensively demonstrated in animal models, application in humans still requires further robust investigation.
32008579	10	69	theme	intervention	1859:1870	arg1	strategies					1872:1881	several intervention strategies	1851:1881	several intervention strategies	1851:1881	Though the influence of gut microbiota on the risk of obesity and several intervention strategies have been extensively demonstrated in animal models, application in humans still requires further robust investigation.
32008579	0	70	theme	dysbiosis-induced	10:26	arg1	obesity					28:34	Microbial dysbiosis-induced obesity	0:34	Microbial dysbiosis-induced obesity	0:34	Microbial dysbiosis-induced obesity: role of gut microbiota in homoeostasis of energy metabolism.
32008579	5	71	theme	intestinal	1032:1041	arg1	integrity					1062:1070	intestinal epithelial barrier integrity	1032:1070	intestinal epithelial barrier integrity	1032:1070	Altered gut microbiota is associated with decreased expression of SCFA that maintain intestinal epithelial barrier integrity, reduce bacterial translocation and inflammation and increase expression of hunger-suppressing hormones.
32008579	6	72	theme	micro-organisms	1207:1221	arg1	amounts					1185:1191	Reduced amounts	1177:1191	Reduced amounts of beneficial micro-organisms	1177:1221	Reduced amounts of beneficial micro-organisms also inhibit fasting-induced adipocyte factor expression leading to dyslipidaemia.
32008579	6	72	theme	micro-organisms	1207:1221	arg1	micro-organisms					1207:1221	beneficial micro-organisms	1196:1221	beneficial micro-organisms	1196:1221	Reduced amounts of beneficial micro-organisms also inhibit fasting-induced adipocyte factor expression leading to dyslipidaemia.
32008579	2	73	theme	dysbiosis-induced	458:474	arg1	inflammation					486:497	dysbiosis-induced metabolic inflammation	458:497	dysbiosis-induced metabolic inflammation	458:497	Therefore, we examined the gut microbial composition and mechanisms of interaction with the host in relation to homoeostatic energy metabolism and pathophysiology of dysbiosis-induced metabolic inflammation and obesity.
32008579	5	74	theme	barrier	1054:1060	arg1	integrity					1062:1070	intestinal epithelial barrier integrity	1032:1070	intestinal epithelial barrier integrity	1032:1070	Altered gut microbiota is associated with decreased expression of SCFA that maintain intestinal epithelial barrier integrity, reduce bacterial translocation and inflammation and increase expression of hunger-suppressing hormones.
32008579	8	75	theme	poor	1568:1571	arg1	control					1583:1589	poor glycaemic control	1568:1589	poor glycaemic control	1568:1589	The synergy of high-fat diet and dysbiotic gut microbiota initiates a recipe that epigenetically programmes the host for increased adiposity and poor glycaemic control.
32008579	7	76	theme	chronic	1318:1324	arg1	inflammation					1326:1337	A low-grade chronic inflammation	1306:1337	A low-grade chronic inflammation (metabolic endotoxaemia)	1306:1362	A low-grade chronic inflammation (metabolic endotoxaemia) ensues which culminates in obesity and its co-morbidities.
32008579	7	76	theme	chronic	1318:1324	arg1	endotoxaemia					1350:1361	metabolic endotoxaemia	1340:1361	metabolic endotoxaemia	1340:1361	A low-grade chronic inflammation (metabolic endotoxaemia) ensues which culminates in obesity and its co-morbidities.
32008579	8	77	theme	dysbiotic	1456:1464	arg1	microbiota					1470:1479	dysbiotic gut microbiota	1456:1479	dysbiotic gut microbiota	1456:1479	The synergy of high-fat diet and dysbiotic gut microbiota initiates a recipe that epigenetically programmes the host for increased adiposity and poor glycaemic control.
32008579	6	78	theme	Reduced	1177:1183	arg1	amounts					1185:1191	Reduced amounts	1177:1191	Reduced amounts of beneficial micro-organisms	1177:1221	Reduced amounts of beneficial micro-organisms also inhibit fasting-induced adipocyte factor expression leading to dyslipidaemia.
32008579	6	78	theme	Reduced	1177:1183	arg1	micro-organisms					1207:1221	beneficial micro-organisms	1196:1221	beneficial micro-organisms	1196:1221	Reduced amounts of beneficial micro-organisms also inhibit fasting-induced adipocyte factor expression leading to dyslipidaemia.
32008579	2	79	theme	obesity	503:509	arg1	pathophysiology					439:453	pathophysiology	439:453	pathophysiology	439:453	Therefore, we examined the gut microbial composition and mechanisms of interaction with the host in relation to homoeostatic energy metabolism and pathophysiology of dysbiosis-induced metabolic inflammation and obesity.
32008579	2	79	theme	obesity	503:509	arg1	metabolism					424:433	homoeostatic energy metabolism	404:433	homoeostatic energy metabolism	404:433	Therefore, we examined the gut microbial composition and mechanisms of interaction with the host in relation to homoeostatic energy metabolism and pathophysiology of dysbiosis-induced metabolic inflammation and obesity.
32008579	2	80	theme	homoeostatic	404:415	arg1	metabolism					424:433	homoeostatic energy metabolism	404:433	homoeostatic energy metabolism	404:433	Therefore, we examined the gut microbial composition and mechanisms of interaction with the host in relation to homoeostatic energy metabolism and pathophysiology of dysbiosis-induced metabolic inflammation and obesity.
32008579	0	81	theme	microbiota	49:58	arg1	role					37:40	role	37:40	role of gut microbiota in homoeostasis of energy metabolism	37:95	Microbial dysbiosis-induced obesity: role of gut microbiota in homoeostasis of energy metabolism.
32008579	5	82	theme	hunger-suppressing	1148:1165	arg1	hormones					1167:1174	hunger-suppressing hormones	1148:1174	hunger-suppressing hormones	1148:1174	Altered gut microbiota is associated with decreased expression of SCFA that maintain intestinal epithelial barrier integrity, reduce bacterial translocation and inflammation and increase expression of hunger-suppressing hormones.
32008579	2	83	theme	inflammation	486:497	arg1	pathophysiology					439:453	pathophysiology	439:453	pathophysiology	439:453	Therefore, we examined the gut microbial composition and mechanisms of interaction with the host in relation to homoeostatic energy metabolism and pathophysiology of dysbiosis-induced metabolic inflammation and obesity.
32008579	2	83	theme	inflammation	486:497	arg1	metabolism					424:433	homoeostatic energy metabolism	404:433	homoeostatic energy metabolism	404:433	Therefore, we examined the gut microbial composition and mechanisms of interaction with the host in relation to homoeostatic energy metabolism and pathophysiology of dysbiosis-induced metabolic inflammation and obesity.
32008579	9	84	from	generation	1677:1686	arg1	transmittable					1654:1666	transmittable	1654:1666	transmittable	1654:1666	Interestingly, these obesogenic mechanistic pathways that are transmittable from one generation to another can be modulated through the administration of probiotics, prebiotics and synbiotics.
32008579	4	85	theme	beneficial	852:861	arg1	Akkermansia					934:944	Akkermansia	934:944	Akkermansia	934:944	High-fat/carbohydrate diet programmes the gut microbiota to one predominated by Firmicutes (Clostridium), Prevotella and Methanobrevibacter but deficient in beneficial genera/species such as Bacteroides, Bifidobacterium, Lactobacillus and Akkermansia.
32008579	4	85	theme	beneficial	852:861	arg1	Lactobacillus					916:928	Lactobacillus	916:928	Lactobacillus	916:928	High-fat/carbohydrate diet programmes the gut microbiota to one predominated by Firmicutes (Clostridium), Prevotella and Methanobrevibacter but deficient in beneficial genera/species such as Bacteroides, Bifidobacterium, Lactobacillus and Akkermansia.
32008579	4	85	theme	beneficial	852:861	arg1	Bifidobacterium					899:913	Bifidobacterium	899:913	Bifidobacterium	899:913	High-fat/carbohydrate diet programmes the gut microbiota to one predominated by Firmicutes (Clostridium), Prevotella and Methanobrevibacter but deficient in beneficial genera/species such as Bacteroides, Bifidobacterium, Lactobacillus and Akkermansia.
32008579	4	85	theme	beneficial	852:861	arg1	genera/species					863:876	beneficial genera/species	852:876	beneficial genera/species such as Bacteroides, Bifidobacterium, Lactobacillus and Akkermansia	852:944	High-fat/carbohydrate diet programmes the gut microbiota to one predominated by Firmicutes (Clostridium), Prevotella and Methanobrevibacter but deficient in beneficial genera/species such as Bacteroides, Bifidobacterium, Lactobacillus and Akkermansia.
32008579	4	85	theme	beneficial	852:861	arg1	Bacteroides					886:896	Bacteroides	886:896	Bacteroides	886:896	High-fat/carbohydrate diet programmes the gut microbiota to one predominated by Firmicutes (Clostridium), Prevotella and Methanobrevibacter but deficient in beneficial genera/species such as Bacteroides, Bifidobacterium, Lactobacillus and Akkermansia.
32008579	2	86	theme	microbial	323:331	arg1	composition					333:343	gut microbial composition	319:343	gut microbial composition	319:343	Therefore, we examined the gut microbial composition and mechanisms of interaction with the host in relation to homoeostatic energy metabolism and pathophysiology of dysbiosis-induced metabolic inflammation and obesity.
32008579	1	87	theme	gut	247:249	arg1	microbiota					251:260	some gut microbiota	242:260	some gut microbiota	242:260	The global obesity epidemic has necessitated the search for better intervention strategies including the exploitation of the health benefits of some gut microbiota and their metabolic products.
34794235	5	0	theme	RSE	747:749	arg1	administration					751:764	RSE administration	747:764	RSE administration	747:764	In addition, RSE administration decreased the relative abundance of the phylum Proteobacteria, which includes many pathogens, and increased the abundance of the genus Akkermansia.
34794235	7	1	from	acids	1128:1132	arg1	RSE					1137:1139	RSE	1137:1139	RSE with colitis-alleviating effects	1137:1172	For the first time, we identified glycosides of sinapic acid as part of hydroxycinnamic acids in RSE with colitis-alleviating effects.
34794235	2	2	from	production	371:380	arg1	macrophages					457:467	lipopolysaccharide-stimulated macrophages	427:467	lipopolysaccharide-stimulated macrophages	427:467	RSE administration was found to effectively inhibit the phosphorylation of IκB and, in turn, the production of pro-inflammatory enzymes and cytokines in lipopolysaccharide-stimulated macrophages.
34794235	0	3	theme	pathway	79:85	arg1	regulation					49:58	regulation	49:58	regulation of NF-kB signaling pathway and modifying gut microbiota	49:114	Radish sprout alleviates DSS-induced colitis via regulation of NF-kB signaling pathway and modifying gut microbiota.
34794235	8	4	theme	nitric	1235:1240	arg1	oxide					1242:1246	nitric oxide	1235:1246	nitric oxide generation in LPS-stimulated macrophages	1235:1287	Notably, 1,2-O-disinapoyl glucoside substantially decreased nitric oxide generation in LPS-stimulated macrophages.
34794235	5	5	theme	relative	780:787	arg1	abundance					789:797	the relative abundance	776:797	the relative abundance of the phylum Proteobacteria, which includes many pathogens	776:857	In addition, RSE administration decreased the relative abundance of the phylum Proteobacteria, which includes many pathogens, and increased the abundance of the genus Akkermansia.
34794235	0	6	theme	gut	101:103	arg1	microbiota					105:114	modifying gut microbiota	91:114	modifying gut microbiota	91:114	Radish sprout alleviates DSS-induced colitis via regulation of NF-kB signaling pathway and modifying gut microbiota.
34794235	7	7	from	RSE	1137:1139	arg1	part					1104:1107	part	1104:1107	part of hydroxycinnamic acids in RSE with colitis-alleviating effects	1104:1172	For the first time, we identified glycosides of sinapic acid as part of hydroxycinnamic acids in RSE with colitis-alleviating effects.
34794235	7	8	theme	acid	1096:1099	arg1	glycosides					1074:1083	glycosides	1074:1083	glycosides of sinapic acid	1074:1099	For the first time, we identified glycosides of sinapic acid as part of hydroxycinnamic acids in RSE with colitis-alleviating effects.
34794235	1	9	from	effects	152:158	arg1	responses					215:223	inflammatory responses	202:223	inflammatory responses in the macrophages	202:242	In this study, we investigated the effects of radish sprout ethanol extract (RSE) on inflammatory responses in the macrophages and a mouse model of colitis.
34794235	1	9	from	effects	152:158	arg1	model					256:260	a mouse model	248:260	a mouse model of colitis	248:271	In this study, we investigated the effects of radish sprout ethanol extract (RSE) on inflammatory responses in the macrophages and a mouse model of colitis.
34794235	3	10	theme	RSE	516:518	arg1	administration					520:533	RSE administration	516:533	RSE administration	516:533	In dextran sulfate sodium (DSS)-colitis mice, RSE administration prevented body weight and colon length reduction, while decreasing inflammation and mucosal necrosis.
34794235	6	11	theme	microbiota	986:995	arg1	composition					997:1007	the gut microbiota composition	978:1007	the gut microbiota composition close to that of healthy mice	978:1037	Beta diversity analyses showed that RSE administration restored the gut microbiota composition close to that of healthy mice.
34794235	0	12	theme	modifying	91:99	arg1	microbiota					105:114	modifying gut microbiota	91:114	modifying gut microbiota	91:114	Radish sprout alleviates DSS-induced colitis via regulation of NF-kB signaling pathway and modifying gut microbiota.
34794235	6	13	theme	gut	982:984	arg1	composition					997:1007	the gut microbiota composition	978:1007	the gut microbiota composition close to that of healthy mice	978:1037	Beta diversity analyses showed that RSE administration restored the gut microbiota composition close to that of healthy mice.
34794235	5	14	theme	genus	895:899	arg1	Akkermansia					901:911	the genus Akkermansia	891:911	the genus Akkermansia	891:911	In addition, RSE administration decreased the relative abundance of the phylum Proteobacteria, which includes many pathogens, and increased the abundance of the genus Akkermansia.
34794235	7	15	theme	colitis-alleviating	1146:1164	arg1	effects					1166:1172	colitis-alleviating effects	1146:1172	colitis-alleviating effects	1146:1172	For the first time, we identified glycosides of sinapic acid as part of hydroxycinnamic acids in RSE with colitis-alleviating effects.
34794235	4	16	theme	fecal	658:662	arg1	microbiota					664:673	the fecal microbiota	654:673	the fecal microbiota	654:673	The diversity of the fecal microbiota was significantly increased in the group treated with RSE.
34794235	0	17	theme	microbiota	105:114	arg1	regulation					49:58	regulation	49:58	regulation of NF-kB signaling pathway and modifying gut microbiota	49:114	Radish sprout alleviates DSS-induced colitis via regulation of NF-kB signaling pathway and modifying gut microbiota.
34794235	7	18	theme	sinapic	1088:1094	arg1	acid					1096:1099	sinapic acid	1088:1099	sinapic acid	1088:1099	For the first time, we identified glycosides of sinapic acid as part of hydroxycinnamic acids in RSE with colitis-alleviating effects.
34794235	7	19	with	RSE	1137:1139	arg1	effects					1166:1172	colitis-alleviating effects	1146:1172	colitis-alleviating effects	1146:1172	For the first time, we identified glycosides of sinapic acid as part of hydroxycinnamic acids in RSE with colitis-alleviating effects.
34794235	1	20	theme	radish	163:168	arg1	RSE					194:196	RSE	194:196	RSE	194:196	In this study, we investigated the effects of radish sprout ethanol extract (RSE) on inflammatory responses in the macrophages and a mouse model of colitis.
34794235	1	20	theme	radish	163:168	arg1	extract					185:191	radish sprout ethanol extract	163:191	radish sprout ethanol extract (RSE)	163:197	In this study, we investigated the effects of radish sprout ethanol extract (RSE) on inflammatory responses in the macrophages and a mouse model of colitis.
34794235	2	21	theme	cytokines	414:422	arg1	production					371:380	the production	367:380	the production of pro-inflammatory enzymes and cytokines in lipopolysaccharide-stimulated macrophages	367:467	RSE administration was found to effectively inhibit the phosphorylation of IκB and, in turn, the production of pro-inflammatory enzymes and cytokines in lipopolysaccharide-stimulated macrophages.
34794235	6	22	theme	RSE	950:952	arg1	administration					954:967	RSE administration	950:967	RSE administration	950:967	Beta diversity analyses showed that RSE administration restored the gut microbiota composition close to that of healthy mice.
34794235	1	23	theme	sprout	170:175	arg1	RSE					194:196	RSE	194:196	RSE	194:196	In this study, we investigated the effects of radish sprout ethanol extract (RSE) on inflammatory responses in the macrophages and a mouse model of colitis.
34794235	1	23	theme	sprout	170:175	arg1	extract					185:191	radish sprout ethanol extract	163:191	radish sprout ethanol extract (RSE)	163:197	In this study, we investigated the effects of radish sprout ethanol extract (RSE) on inflammatory responses in the macrophages and a mouse model of colitis.
34794235	0	24	theme	Radish	0:5	arg1	sprout					7:12	Radish sprout	0:12	Radish sprout	0:12	Radish sprout alleviates DSS-induced colitis via regulation of NF-kB signaling pathway and modifying gut microbiota.
34794235	1	25	theme	mouse	250:254	arg1	model					256:260	a mouse model	248:260	a mouse model of colitis	248:271	In this study, we investigated the effects of radish sprout ethanol extract (RSE) on inflammatory responses in the macrophages and a mouse model of colitis.
34794235	1	26	from	model	256:260	arg1	macrophages					232:242	the macrophages	228:242	the macrophages	228:242	In this study, we investigated the effects of radish sprout ethanol extract (RSE) on inflammatory responses in the macrophages and a mouse model of colitis.
34794235	1	27	theme	ethanol	177:183	arg1	RSE					194:196	RSE	194:196	RSE	194:196	In this study, we investigated the effects of radish sprout ethanol extract (RSE) on inflammatory responses in the macrophages and a mouse model of colitis.
34794235	1	27	theme	ethanol	177:183	arg1	extract					185:191	radish sprout ethanol extract	163:191	radish sprout ethanol extract (RSE)	163:197	In this study, we investigated the effects of radish sprout ethanol extract (RSE) on inflammatory responses in the macrophages and a mouse model of colitis.
34794235	0	28	theme	DSS-induced	25:35	arg1	colitis					37:43	DSS-induced colitis	25:43	DSS-induced colitis	25:43	Radish sprout alleviates DSS-induced colitis via regulation of NF-kB signaling pathway and modifying gut microbiota.
34794235	2	29	theme	RSE	274:276	arg1	administration					278:291	RSE administration	274:291	RSE administration	274:291	RSE administration was found to effectively inhibit the phosphorylation of IκB and, in turn, the production of pro-inflammatory enzymes and cytokines in lipopolysaccharide-stimulated macrophages.
34794235	1	30	theme	extract	185:191	arg1	effects					152:158	the effects	148:158	the effects of radish sprout ethanol extract (RSE) on inflammatory responses in the macrophages and a mouse model of colitis	148:271	In this study, we investigated the effects of radish sprout ethanol extract (RSE) on inflammatory responses in the macrophages and a mouse model of colitis.
34794235	2	31	theme	pro-inflammatory	385:400	arg1	enzymes					402:408	pro-inflammatory enzymes	385:408	pro-inflammatory enzymes	385:408	RSE administration was found to effectively inhibit the phosphorylation of IκB and, in turn, the production of pro-inflammatory enzymes and cytokines in lipopolysaccharide-stimulated macrophages.
34794235	3	32	theme	body	545:548	arg1	weight					550:555	body weight	545:555	body weight	545:555	In dextran sulfate sodium (DSS)-colitis mice, RSE administration prevented body weight and colon length reduction, while decreasing inflammation and mucosal necrosis.
34794235	1	33	theme	colitis	265:271	arg1	responses					215:223	inflammatory responses	202:223	inflammatory responses in the macrophages	202:242	In this study, we investigated the effects of radish sprout ethanol extract (RSE) on inflammatory responses in the macrophages and a mouse model of colitis.
34794235	1	33	theme	colitis	265:271	arg1	model					256:260	a mouse model	248:260	a mouse model of colitis	248:271	In this study, we investigated the effects of radish sprout ethanol extract (RSE) on inflammatory responses in the macrophages and a mouse model of colitis.
34794235	7	34	theme	first	1048:1052	arg1	time					1054:1057	the first time	1044:1057	the first time	1044:1057	For the first time, we identified glycosides of sinapic acid as part of hydroxycinnamic acids in RSE with colitis-alleviating effects.
34794235	8	35	theme	LPS-stimulated	1262:1275	arg1	macrophages					1277:1287	LPS-stimulated macrophages	1262:1287	LPS-stimulated macrophages	1262:1287	Notably, 1,2-O-disinapoyl glucoside substantially decreased nitric oxide generation in LPS-stimulated macrophages.
34794235	7	36	theme	hydroxycinnamic	1112:1126	arg1	acids					1128:1132	hydroxycinnamic acids	1112:1132	hydroxycinnamic acids in RSE with colitis-alleviating effects	1112:1172	For the first time, we identified glycosides of sinapic acid as part of hydroxycinnamic acids in RSE with colitis-alleviating effects.
34794235	3	37	theme	mucosal	619:625	arg1	necrosis					627:634	mucosal necrosis	619:634	mucosal necrosis	619:634	In dextran sulfate sodium (DSS)-colitis mice, RSE administration prevented body weight and colon length reduction, while decreasing inflammation and mucosal necrosis.
34794235	2	38	theme	enzymes	402:408	arg1	production					371:380	the production	367:380	the production of pro-inflammatory enzymes and cytokines in lipopolysaccharide-stimulated macrophages	367:467	RSE administration was found to effectively inhibit the phosphorylation of IκB and, in turn, the production of pro-inflammatory enzymes and cytokines in lipopolysaccharide-stimulated macrophages.
34794235	5	39	theme	phylum	806:811	arg1	Proteobacteria					813:826	the phylum Proteobacteria	802:826	the phylum Proteobacteria	802:826	In addition, RSE administration decreased the relative abundance of the phylum Proteobacteria, which includes many pathogens, and increased the abundance of the genus Akkermansia.
34794235	2	40	theme	lipopolysaccharide-stimulated	427:455	arg1	macrophages					457:467	lipopolysaccharide-stimulated macrophages	427:467	lipopolysaccharide-stimulated macrophages	427:467	RSE administration was found to effectively inhibit the phosphorylation of IκB and, in turn, the production of pro-inflammatory enzymes and cytokines in lipopolysaccharide-stimulated macrophages.
34794235	1	41	from	responses	215:223	arg1	macrophages					232:242	the macrophages	228:242	the macrophages	228:242	In this study, we investigated the effects of radish sprout ethanol extract (RSE) on inflammatory responses in the macrophages and a mouse model of colitis.
34794235	3	42	theme	colon	561:565	arg1	reduction					574:582	colon length reduction	561:582	colon length reduction	561:582	In dextran sulfate sodium (DSS)-colitis mice, RSE administration prevented body weight and colon length reduction, while decreasing inflammation and mucosal necrosis.
34794235	3	43	theme	sulfate	481:487	arg1	DSS					497:499	DSS	497:499	DSS	497:499	In dextran sulfate sodium (DSS)-colitis mice, RSE administration prevented body weight and colon length reduction, while decreasing inflammation and mucosal necrosis.
34794235	3	43	theme	sulfate	481:487	arg1	sodium					489:494	dextran sulfate sodium	473:494	dextran sulfate sodium (DSS)-colitis mice	473:513	In dextran sulfate sodium (DSS)-colitis mice, RSE administration prevented body weight and colon length reduction, while decreasing inflammation and mucosal necrosis.
34794235	7	44	theme	acids	1128:1132	arg1	part					1104:1107	part	1104:1107	part of hydroxycinnamic acids in RSE with colitis-alleviating effects	1104:1172	For the first time, we identified glycosides of sinapic acid as part of hydroxycinnamic acids in RSE with colitis-alleviating effects.
34794235	7	45	from	part	1104:1107	arg1	RSE					1137:1139	RSE	1137:1139	RSE with colitis-alleviating effects	1137:1172	For the first time, we identified glycosides of sinapic acid as part of hydroxycinnamic acids in RSE with colitis-alleviating effects.
34794235	3	46	theme	length	567:572	arg1	reduction					574:582	colon length reduction	561:582	colon length reduction	561:582	In dextran sulfate sodium (DSS)-colitis mice, RSE administration prevented body weight and colon length reduction, while decreasing inflammation and mucosal necrosis.
34794235	3	47	theme	sodium	489:494	arg1	mice					510:513	dextran sulfate sodium (DSS)-colitis mice	473:513	dextran sulfate sodium (DSS)-colitis mice	473:513	In dextran sulfate sodium (DSS)-colitis mice, RSE administration prevented body weight and colon length reduction, while decreasing inflammation and mucosal necrosis.
34794235	6	48	theme	diversity	919:927	arg1	analyses					929:936	Beta diversity analyses	914:936	Beta diversity analyses	914:936	Beta diversity analyses showed that RSE administration restored the gut microbiota composition close to that of healthy mice.
34794235	5	49	theme	Akkermansia	901:911	arg1	abundance					878:886	the abundance	874:886	the abundance of the genus Akkermansia	874:911	In addition, RSE administration decreased the relative abundance of the phylum Proteobacteria, which includes many pathogens, and increased the abundance of the genus Akkermansia.
34794235	4	50	theme	microbiota	664:673	arg1	diversity					641:649	The diversity	637:649	The diversity of the fecal microbiota	637:673	The diversity of the fecal microbiota was significantly increased in the group treated with RSE.
34794235	8	51	theme	1,2-O-disinapoyl	1184:1199	arg1	glucoside					1201:1209	1,2-O-disinapoyl glucoside	1184:1209	1,2-O-disinapoyl glucoside	1184:1209	Notably, 1,2-O-disinapoyl glucoside substantially decreased nitric oxide generation in LPS-stimulated macrophages.
34794235	6	52	theme	healthy	1026:1032	arg1	mice					1034:1037	healthy mice	1026:1037	healthy mice	1026:1037	Beta diversity analyses showed that RSE administration restored the gut microbiota composition close to that of healthy mice.
34794235	8	53	from	generation	1248:1257	arg1	macrophages					1277:1287	LPS-stimulated macrophages	1262:1287	LPS-stimulated macrophages	1262:1287	Notably, 1,2-O-disinapoyl glucoside substantially decreased nitric oxide generation in LPS-stimulated macrophages.
34794235	3	54	theme	dextran	473:479	arg1	DSS					497:499	DSS	497:499	DSS	497:499	In dextran sulfate sodium (DSS)-colitis mice, RSE administration prevented body weight and colon length reduction, while decreasing inflammation and mucosal necrosis.
34794235	3	54	theme	dextran	473:479	arg1	sodium					489:494	dextran sulfate sodium	473:494	dextran sulfate sodium (DSS)-colitis mice	473:513	In dextran sulfate sodium (DSS)-colitis mice, RSE administration prevented body weight and colon length reduction, while decreasing inflammation and mucosal necrosis.
34794235	8	55	theme	oxide	1242:1246	arg1	generation					1248:1257	nitric oxide generation	1235:1257	nitric oxide generation in LPS-stimulated macrophages	1235:1287	Notably, 1,2-O-disinapoyl glucoside substantially decreased nitric oxide generation in LPS-stimulated macrophages.
34794235	1	56	theme	inflammatory	202:213	arg1	responses					215:223	inflammatory responses	202:223	inflammatory responses in the macrophages	202:242	In this study, we investigated the effects of radish sprout ethanol extract (RSE) on inflammatory responses in the macrophages and a mouse model of colitis.
34794235	0	57	theme	signaling	69:77	arg1	pathway					79:85	NF-kB signaling pathway	63:85	NF-kB signaling pathway	63:85	Radish sprout alleviates DSS-induced colitis via regulation of NF-kB signaling pathway and modifying gut microbiota.
34794235	5	58	theme	many	844:847	arg1	pathogens					849:857	many pathogens	844:857	many pathogens	844:857	In addition, RSE administration decreased the relative abundance of the phylum Proteobacteria, which includes many pathogens, and increased the abundance of the genus Akkermansia.
34794235	2	59	theme	IκB	349:351	arg1	phosphorylation					330:344	the phosphorylation	326:344	the phosphorylation of IκB	326:351	RSE administration was found to effectively inhibit the phosphorylation of IκB and, in turn, the production of pro-inflammatory enzymes and cytokines in lipopolysaccharide-stimulated macrophages.
34794235	0	60	theme	NF-kB	63:67	arg1	pathway					79:85	NF-kB signaling pathway	63:85	NF-kB signaling pathway	63:85	Radish sprout alleviates DSS-induced colitis via regulation of NF-kB signaling pathway and modifying gut microbiota.
34794235	6	61	theme	Beta	914:917	arg1	analyses					929:936	Beta diversity analyses	914:936	Beta diversity analyses	914:936	Beta diversity analyses showed that RSE administration restored the gut microbiota composition close to that of healthy mice.
34794235	5	62	theme	Proteobacteria	813:826	arg1	abundance					789:797	the relative abundance	776:797	the relative abundance of the phylum Proteobacteria, which includes many pathogens	776:857	In addition, RSE administration decreased the relative abundance of the phylum Proteobacteria, which includes many pathogens, and increased the abundance of the genus Akkermansia.
34794235	3	63	theme	-colitis	501:508	arg1	mice					510:513	dextran sulfate sodium (DSS)-colitis mice	473:513	dextran sulfate sodium (DSS)-colitis mice	473:513	In dextran sulfate sodium (DSS)-colitis mice, RSE administration prevented body weight and colon length reduction, while decreasing inflammation and mucosal necrosis.
32289500	8	0	theme	short-chain	1212:1222	arg1	acids					1230:1234	fecal short-chain fatty acids	1206:1234	fecal short-chain fatty acids (SCFAs)	1206:1242	The microbiome composition was evaluated via 16S rRNA gene variable region V4 amplicon sequencing, whereas fecal short-chain fatty acids (SCFAs) were assessed by using targeted mass spectrometry.
32289500	8	0	theme	short-chain	1212:1222	arg1	SCFAs					1237:1241	SCFAs	1237:1241	SCFAs	1237:1241	The microbiome composition was evaluated via 16S rRNA gene variable region V4 amplicon sequencing, whereas fecal short-chain fatty acids (SCFAs) were assessed by using targeted mass spectrometry.
32289500	3	1	theme	transient	574:582	arg1	function					603:610	transient receptor potential function	574:610	transient receptor potential function	574:610	Because inflammatory bowel disease patients in remission exhibit dysbiosis and microbial manipulation alters TRPV1 function, our goal was to examine whether microbial perturbation modulated transient receptor potential function in a mouse model.
32289500	6	2	theme	Frey	967:970	arg1	tests					972:976	hot plate/automated Von Frey tests	943:976	hot plate/automated Von Frey tests	943:976	Visceral and somatic sensitivity were assessed by recording visceromotor responses to colorectal distention and using hot plate/automated Von Frey tests, respectively.
32289500	4	3	theme	sodium	662:667	arg1	DSS					678:680	DSS	678:680	DSS	678:680	METHODS Mice were given dextran sodium sulfate (DSS) to induce colitis and were allowed to recover.
32289500	4	3	theme	sodium	662:667	arg1	sulfate					669:675	dextran sodium sulfate	654:675	dextran sodium sulfate (DSS)	654:681	METHODS Mice were given dextran sodium sulfate (DSS) to induce colitis and were allowed to recover.
32289500	11	4	theme	DSS	1521:1523	arg1	stool					1525:1529	postinflammatory DSS stool	1504:1529	postinflammatory DSS stool	1504:1529	FMT of postinflammatory DSS stool into antibiotic-treated mice increased visceral hypersensitivity, whereas FMT of control stool reversed antibiotics' sensitizing effects.
32289500	11	5	theme	sensitizing	1648:1658	arg1	effects					1660:1666	antibiotics' sensitizing effects	1635:1666	antibiotics' sensitizing effects	1635:1666	FMT of postinflammatory DSS stool into antibiotic-treated mice increased visceral hypersensitivity, whereas FMT of control stool reversed antibiotics' sensitizing effects.
32289500	3	6	theme	mouse	617:621	arg1	model					623:627	a mouse model	615:627	a mouse model	615:627	Because inflammatory bowel disease patients in remission exhibit dysbiosis and microbial manipulation alters TRPV1 function, our goal was to examine whether microbial perturbation modulated transient receptor potential function in a mouse model.
32289500	6	7	theme	plate/automated	947:961	arg1	tests					972:976	hot plate/automated Von Frey tests	943:976	hot plate/automated Von Frey tests	943:976	Visceral and somatic sensitivity were assessed by recording visceromotor responses to colorectal distention and using hot plate/automated Von Frey tests, respectively.
32289500	13	8	theme	colonic	1948:1954	arg1	supernatants					1956:1967	colonic supernatants	1948:1967	colonic supernatants derived from postinflammatory mice	1948:2002	Capsaicin-evoked calcium responses were increased in naive dorsal root ganglion neurons incubated with both sodium butyrate/propionate alone and with colonic supernatants derived from postinflammatory mice.
32289500	2	9	theme	key	347:349	arg1	expression					333:342	rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression	268:342	rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression	268:342	These patients have increased rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression, a key transducer of inflammatory pain.
32289500	2	9	theme	key	347:349	arg1	transducer					351:360	a key transducer	345:360	a key transducer of inflammatory pain	345:381	These patients have increased rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression, a key transducer of inflammatory pain.
32289500	1	10	theme	abdominal	222:230	arg1	pain					232:235	chronic abdominal pain	214:235	chronic abdominal pain	214:235	BACKGROUND & AIMS Despite achieving endoscopic remission, more than 20% of inflammatory bowel disease patients experience chronic abdominal pain.
32289500	12	11	theme	acetate/butyrate	1749:1764	arg1	content					1766:1772	fecal acetate/butyrate content	1743:1772	fecal acetate/butyrate content	1743:1772	Postinflammatory mice exhibited both increased SCFA-producing species and fecal acetate/butyrate content compared with controls.
32289500	8	12	theme	targeted	1267:1274	arg1	spectrometry					1281:1292	targeted mass spectrometry	1267:1292	targeted mass spectrometry	1267:1292	The microbiome composition was evaluated via 16S rRNA gene variable region V4 amplicon sequencing, whereas fecal short-chain fatty acids (SCFAs) were assessed by using targeted mass spectrometry.
32289500	9	13	theme	Postinflammatory	1303:1318	arg1	mice					1324:1327	Postinflammatory DSS mice	1303:1327	Postinflammatory DSS mice	1303:1327	RESULTS Postinflammatory DSS mice developed visceral and somatic hyperalgesia.
32289500	2	14	theme	rectal	268:273	arg1	expression					333:342	rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression	268:342	rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression	268:342	These patients have increased rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression, a key transducer of inflammatory pain.
32289500	2	14	theme	rectal	268:273	arg1	transducer					351:360	a key transducer	345:360	a key transducer of inflammatory pain	345:381	These patients have increased rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression, a key transducer of inflammatory pain.
32289500	12	15	theme	SCFA-producing	1716:1729	arg1	species					1731:1737	increased SCFA-producing species	1706:1737	increased SCFA-producing species	1706:1737	Postinflammatory mice exhibited both increased SCFA-producing species and fecal acetate/butyrate content compared with controls.
32289500	1	16	dep	AIMS	105:108	arg1	experience					203:212	experience	203:212	experience chronic abdominal pain	203:235	BACKGROUND & AIMS Despite achieving endoscopic remission, more than 20% of inflammatory bowel disease patients experience chronic abdominal pain.
32289500	11	17	theme	control	1612:1618	arg1	stool					1620:1624	control stool	1612:1624	control stool	1612:1624	FMT of postinflammatory DSS stool into antibiotic-treated mice increased visceral hypersensitivity, whereas FMT of control stool reversed antibiotics' sensitizing effects.
32289500	9	18	theme	visceral	1339:1346	arg1	hyperalgesia					1360:1371	visceral and somatic hyperalgesia	1339:1371	visceral and somatic hyperalgesia	1339:1371	RESULTS Postinflammatory DSS mice developed visceral and somatic hyperalgesia.
32289500	2	19	theme	vanilloid-1	304:314	arg1	expression					333:342	rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression	268:342	rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression	268:342	These patients have increased rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression, a key transducer of inflammatory pain.
32289500	2	19	theme	vanilloid-1	304:314	arg1	transducer					351:360	a key transducer	345:360	a key transducer of inflammatory pain	345:381	These patients have increased rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression, a key transducer of inflammatory pain.
32289500	14	20	theme	postinflammatory	2056:2071	arg1	hypersensitivity					2082:2097	postinflammatory visceral hypersensitivity	2056:2097	postinflammatory visceral hypersensitivity	2056:2097	CONCLUSIONS The microbiome plays a central role in postinflammatory visceral hypersensitivity.
32289500	9	21	theme	somatic	1352:1358	arg1	hyperalgesia					1360:1371	visceral and somatic hyperalgesia	1339:1371	visceral and somatic hyperalgesia	1339:1371	RESULTS Postinflammatory DSS mice developed visceral and somatic hyperalgesia.
32289500	2	22	theme	receptor	285:292	arg1	expression					333:342	rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression	268:342	rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression	268:342	These patients have increased rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression, a key transducer of inflammatory pain.
32289500	2	22	theme	receptor	285:292	arg1	transducer					351:360	a key transducer	345:360	a key transducer of inflammatory pain	345:381	These patients have increased rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression, a key transducer of inflammatory pain.
32289500	7	23	theme	neurons	1041:1047	arg1	imaging					1001:1007	Calcium imaging	993:1007	Calcium imaging of isolated dorsal root ganglia neurons	993:1047	Calcium imaging of isolated dorsal root ganglia neurons was used as an in vitro correlate of nociception.
32289500	7	23	theme	neurons	1041:1047	arg1	correlate					1073:1081	an in vitro correlate	1061:1081	an in vitro correlate of nociception	1061:1096	Calcium imaging of isolated dorsal root ganglia neurons was used as an in vitro correlate of nociception.
32289500	1	24	theme	inflammatory	167:178	arg1	disease					186:192	inflammatory bowel disease	167:192	inflammatory bowel disease patients	167:201	BACKGROUND & AIMS Despite achieving endoscopic remission, more than 20% of inflammatory bowel disease patients experience chronic abdominal pain.
32289500	13	25	theme	postinflammatory	1982:1997	arg1	mice					1999:2002	postinflammatory mice	1982:2002	postinflammatory mice	1982:2002	Capsaicin-evoked calcium responses were increased in naive dorsal root ganglion neurons incubated with both sodium butyrate/propionate alone and with colonic supernatants derived from postinflammatory mice.
32289500	15	26	theme	visceral	2217:2224	arg1	pain					2226:2229	postinflammatory visceral pain	2200:2229	postinflammatory visceral pain	2200:2229	Microbial-derived SCFAs can sensitize nociceptive neurons and may contribute to the pathogenesis of postinflammatory visceral pain.
32289500	7	27	theme	root	1028:1031	arg1	neurons					1041:1047	isolated dorsal root ganglia neurons	1012:1047	isolated dorsal root ganglia neurons	1012:1047	Calcium imaging of isolated dorsal root ganglia neurons was used as an in vitro correlate of nociception.
32289500	1	28	theme	disease	186:192	arg1	patients					194:201	inflammatory bowel disease patients	167:201	inflammatory bowel disease patients	167:201	BACKGROUND & AIMS Despite achieving endoscopic remission, more than 20% of inflammatory bowel disease patients experience chronic abdominal pain.
32289500	3	29	theme	bowel	405:409	arg1	disease					411:417	inflammatory bowel disease	392:417	inflammatory bowel disease patients in remission	392:439	Because inflammatory bowel disease patients in remission exhibit dysbiosis and microbial manipulation alters TRPV1 function, our goal was to examine whether microbial perturbation modulated transient receptor potential function in a mouse model.
32289500	3	30	from	patients	419:426	arg1	remission					431:439	remission	431:439	remission	431:439	Because inflammatory bowel disease patients in remission exhibit dysbiosis and microbial manipulation alters TRPV1 function, our goal was to examine whether microbial perturbation modulated transient receptor potential function in a mouse model.
32289500	7	31	theme	isolated	1012:1019	arg1	neurons					1041:1047	isolated dorsal root ganglia neurons	1012:1047	isolated dorsal root ganglia neurons	1012:1047	Calcium imaging of isolated dorsal root ganglia neurons was used as an in vitro correlate of nociception.
32289500	1	32	theme	&	103:103	arg1	AIMS					105:108	BACKGROUND & AIMS	92:108	BACKGROUND & AIMS Despite achieving endoscopic remission, more than 20% of inflammatory bowel disease patients experience chronic abdominal pain.	92:236	BACKGROUND & AIMS Despite achieving endoscopic remission, more than 20% of inflammatory bowel disease patients experience chronic abdominal pain.
32289500	13	33	theme	naive	1851:1855	arg1	neurons					1878:1884	naive dorsal root ganglion neurons	1851:1884	naive dorsal root ganglion neurons incubated with both sodium butyrate/propionate alone and with colonic supernatants derived from postinflammatory mice	1851:2002	Capsaicin-evoked calcium responses were increased in naive dorsal root ganglion neurons incubated with both sodium butyrate/propionate alone and with colonic supernatants derived from postinflammatory mice.
32289500	5	34	theme	fecal	791:795	arg1	transplant					807:816	fecal microbial transplant	791:816	antibiotics as well as fecal microbial transplant (FMT)	768:822	The microbiome was perturbed by using antibiotics as well as fecal microbial transplant (FMT).
32289500	5	34	theme	fecal	791:795	arg1	FMT					819:821	FMT	819:821	FMT	819:821	The microbiome was perturbed by using antibiotics as well as fecal microbial transplant (FMT).
32289500	13	35	theme	root	1864:1867	arg1	neurons					1878:1884	naive dorsal root ganglion neurons	1851:1884	naive dorsal root ganglion neurons incubated with both sodium butyrate/propionate alone and with colonic supernatants derived from postinflammatory mice	1851:2002	Capsaicin-evoked calcium responses were increased in naive dorsal root ganglion neurons incubated with both sodium butyrate/propionate alone and with colonic supernatants derived from postinflammatory mice.
32289500	0	36	theme	Colitis-Induced	0:14	arg1	Perturbation					26:37	Colitis-Induced Microbial Perturbation	0:37	Colitis-Induced Microbial Perturbation	0:37	Colitis-Induced Microbial Perturbation Promotes Postinflammatory Visceral Hypersensitivity.
32289500	13	37	dep	butyrate/propionate	1913:1931	arg1	both					1901:1904	both	1901:1904	both	1901:1904	Capsaicin-evoked calcium responses were increased in naive dorsal root ganglion neurons incubated with both sodium butyrate/propionate alone and with colonic supernatants derived from postinflammatory mice.
32289500	1	38	theme	endoscopic	128:137	arg1	remission					139:147	endoscopic remission	128:147	endoscopic remission	128:147	BACKGROUND & AIMS Despite achieving endoscopic remission, more than 20% of inflammatory bowel disease patients experience chronic abdominal pain.
32289500	10	39	theme	independent	1468:1478	arg1	hyperalgesia					1455:1466	visceral, but not somatic, hyperalgesia	1428:1466	visceral, but not somatic, hyperalgesia independent of inflammation	1428:1494	Antibiotic administration during DSS recovery induced visceral, but not somatic, hyperalgesia independent of inflammation.
32289500	10	40	theme	DSS	1407:1409	arg1	recovery					1411:1418	DSS recovery	1407:1418	DSS recovery	1407:1418	Antibiotic administration during DSS recovery induced visceral, but not somatic, hyperalgesia independent of inflammation.
32289500	3	41	theme	microbial	463:471	arg1	manipulation					473:484	microbial manipulation	463:484	microbial manipulation	463:484	Because inflammatory bowel disease patients in remission exhibit dysbiosis and microbial manipulation alters TRPV1 function, our goal was to examine whether microbial perturbation modulated transient receptor potential function in a mouse model.
32289500	4	42	theme	METHODS	630:636	arg1	Mice					638:641	METHODS Mice	630:641	METHODS Mice	630:641	METHODS Mice were given dextran sodium sulfate (DSS) to induce colitis and were allowed to recover.
32289500	15	43	link	Microbial-derived	2100:2116	arg1	SCFAs					2118:2122	Microbial-derived SCFAs	2100:2122	Microbial-derived SCFAs	2100:2122	Microbial-derived SCFAs can sensitize nociceptive neurons and may contribute to the pathogenesis of postinflammatory visceral pain.
32289500	11	44	theme	visceral	1570:1577	arg1	hypersensitivity					1579:1594	visceral hypersensitivity	1570:1594	visceral hypersensitivity	1570:1594	FMT of postinflammatory DSS stool into antibiotic-treated mice increased visceral hypersensitivity, whereas FMT of control stool reversed antibiotics' sensitizing effects.
32289500	0	45	theme	Postinflammatory	48:63	arg1	Hypersensitivity					74:89	Postinflammatory Visceral Hypersensitivity	48:89	Postinflammatory Visceral Hypersensitivity	48:89	Colitis-Induced Microbial Perturbation Promotes Postinflammatory Visceral Hypersensitivity.
32289500	8	46	theme	gene	1153:1156	arg1	sequencing					1186:1195	rRNA gene variable region V4 amplicon sequencing	1148:1195	rRNA gene variable region V4 amplicon sequencing	1148:1195	The microbiome composition was evaluated via 16S rRNA gene variable region V4 amplicon sequencing, whereas fecal short-chain fatty acids (SCFAs) were assessed by using targeted mass spectrometry.
32289500	6	47	theme	colorectal	911:920	arg1	distention					922:931	colorectal distention	911:931	colorectal distention	911:931	Visceral and somatic sensitivity were assessed by recording visceromotor responses to colorectal distention and using hot plate/automated Von Frey tests, respectively.
32289500	3	48	theme	microbial	541:549	arg1	perturbation					551:562	microbial perturbation	541:562	microbial perturbation	541:562	Because inflammatory bowel disease patients in remission exhibit dysbiosis and microbial manipulation alters TRPV1 function, our goal was to examine whether microbial perturbation modulated transient receptor potential function in a mouse model.
32289500	8	49	theme	region	1167:1172	arg1	sequencing					1186:1195	rRNA gene variable region V4 amplicon sequencing	1148:1195	rRNA gene variable region V4 amplicon sequencing	1148:1195	The microbiome composition was evaluated via 16S rRNA gene variable region V4 amplicon sequencing, whereas fecal short-chain fatty acids (SCFAs) were assessed by using targeted mass spectrometry.
32289500	13	50	theme	Capsaicin-evoked	1798:1813	arg1	responses					1823:1831	Capsaicin-evoked calcium responses	1798:1831	Capsaicin-evoked calcium responses	1798:1831	Capsaicin-evoked calcium responses were increased in naive dorsal root ganglion neurons incubated with both sodium butyrate/propionate alone and with colonic supernatants derived from postinflammatory mice.
32289500	2	51	theme	inflammatory	365:376	arg1	pain					378:381	inflammatory pain	365:381	inflammatory pain	365:381	These patients have increased rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression, a key transducer of inflammatory pain.
32289500	8	52	theme	amplicon	1177:1184	arg1	sequencing					1186:1195	rRNA gene variable region V4 amplicon sequencing	1148:1195	rRNA gene variable region V4 amplicon sequencing	1148:1195	The microbiome composition was evaluated via 16S rRNA gene variable region V4 amplicon sequencing, whereas fecal short-chain fatty acids (SCFAs) were assessed by using targeted mass spectrometry.
32289500	3	53	theme	receptor	584:591	arg1	function					603:610	transient receptor potential function	574:610	transient receptor potential function	574:610	Because inflammatory bowel disease patients in remission exhibit dysbiosis and microbial manipulation alters TRPV1 function, our goal was to examine whether microbial perturbation modulated transient receptor potential function in a mouse model.
32289500	9	54	dep	RESULTS	1295:1301	arg1	developed					1329:1337	developed	1329:1337	developed visceral and somatic hyperalgesia	1329:1371	RESULTS Postinflammatory DSS mice developed visceral and somatic hyperalgesia.
32289500	8	55	theme	fecal	1206:1210	arg1	acids					1230:1234	fecal short-chain fatty acids	1206:1234	fecal short-chain fatty acids (SCFAs)	1206:1242	The microbiome composition was evaluated via 16S rRNA gene variable region V4 amplicon sequencing, whereas fecal short-chain fatty acids (SCFAs) were assessed by using targeted mass spectrometry.
32289500	8	55	theme	fecal	1206:1210	arg1	SCFAs					1237:1241	SCFAs	1237:1241	SCFAs	1237:1241	The microbiome composition was evaluated via 16S rRNA gene variable region V4 amplicon sequencing, whereas fecal short-chain fatty acids (SCFAs) were assessed by using targeted mass spectrometry.
32289500	11	56	theme	postinflammatory	1504:1519	arg1	stool					1525:1529	postinflammatory DSS stool	1504:1529	postinflammatory DSS stool	1504:1529	FMT of postinflammatory DSS stool into antibiotic-treated mice increased visceral hypersensitivity, whereas FMT of control stool reversed antibiotics' sensitizing effects.
32289500	10	57	theme	inflammation	1483:1494	arg1	independent					1468:1478	independent	1468:1478	independent	1468:1478	Antibiotic administration during DSS recovery induced visceral, but not somatic, hyperalgesia independent of inflammation.
32289500	8	58	theme	fatty	1224:1228	arg1	acids					1230:1234	fecal short-chain fatty acids	1206:1234	fecal short-chain fatty acids (SCFAs)	1206:1242	The microbiome composition was evaluated via 16S rRNA gene variable region V4 amplicon sequencing, whereas fecal short-chain fatty acids (SCFAs) were assessed by using targeted mass spectrometry.
32289500	8	58	theme	fatty	1224:1228	arg1	SCFAs					1237:1241	SCFAs	1237:1241	SCFAs	1237:1241	The microbiome composition was evaluated via 16S rRNA gene variable region V4 amplicon sequencing, whereas fecal short-chain fatty acids (SCFAs) were assessed by using targeted mass spectrometry.
32289500	6	59	theme	Von	963:965	arg1	tests					972:976	hot plate/automated Von Frey tests	943:976	hot plate/automated Von Frey tests	943:976	Visceral and somatic sensitivity were assessed by recording visceromotor responses to colorectal distention and using hot plate/automated Von Frey tests, respectively.
32289500	14	60	theme	central	2040:2046	arg1	role					2048:2051	a central role	2038:2051	a central role	2038:2051	CONCLUSIONS The microbiome plays a central role in postinflammatory visceral hypersensitivity.
32289500	4	61	theme	dextran	654:660	arg1	DSS					678:680	DSS	678:680	DSS	678:680	METHODS Mice were given dextran sodium sulfate (DSS) to induce colitis and were allowed to recover.
32289500	4	61	theme	dextran	654:660	arg1	sulfate					669:675	dextran sodium sulfate	654:675	dextran sodium sulfate (DSS)	654:681	METHODS Mice were given dextran sodium sulfate (DSS) to induce colitis and were allowed to recover.
32289500	6	62	theme	somatic	838:844	arg1	sensitivity					846:856	Visceral and somatic sensitivity	825:856	Visceral and somatic sensitivity	825:856	Visceral and somatic sensitivity were assessed by recording visceromotor responses to colorectal distention and using hot plate/automated Von Frey tests, respectively.
32289500	2	63	theme	TRPV1	326:330	arg1	expression					333:342	rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression	268:342	rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression	268:342	These patients have increased rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression, a key transducer of inflammatory pain.
32289500	2	63	theme	TRPV1	326:330	arg1	transducer					351:360	a key transducer	345:360	a key transducer of inflammatory pain	345:381	These patients have increased rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression, a key transducer of inflammatory pain.
32289500	1	64	theme	patients	194:201	arg1	patients					194:201	inflammatory bowel disease patients	167:201	inflammatory bowel disease patients	167:201	BACKGROUND & AIMS Despite achieving endoscopic remission, more than 20% of inflammatory bowel disease patients experience chronic abdominal pain.
32289500	1	64	theme	patients	194:201	arg1	%					162:162	more than 20%	150:162	more than 20% of inflammatory bowel disease patients	150:201	BACKGROUND & AIMS Despite achieving endoscopic remission, more than 20% of inflammatory bowel disease patients experience chronic abdominal pain.
32289500	6	65	theme	hot	943:945	arg1	tests					972:976	hot plate/automated Von Frey tests	943:976	hot plate/automated Von Frey tests	943:976	Visceral and somatic sensitivity were assessed by recording visceromotor responses to colorectal distention and using hot plate/automated Von Frey tests, respectively.
32289500	6	66	theme	Visceral	825:832	arg1	sensitivity					846:856	Visceral and somatic sensitivity	825:856	Visceral and somatic sensitivity	825:856	Visceral and somatic sensitivity were assessed by recording visceromotor responses to colorectal distention and using hot plate/automated Von Frey tests, respectively.
32289500	1	67	theme	chronic	214:220	arg1	pain					232:235	chronic abdominal pain	214:235	chronic abdominal pain	214:235	BACKGROUND & AIMS Despite achieving endoscopic remission, more than 20% of inflammatory bowel disease patients experience chronic abdominal pain.
32289500	15	68	theme	nociceptive	2138:2148	arg1	neurons					2150:2156	nociceptive neurons	2138:2156	nociceptive neurons	2138:2156	Microbial-derived SCFAs can sensitize nociceptive neurons and may contribute to the pathogenesis of postinflammatory visceral pain.
32289500	13	69	theme	sodium	1906:1911	arg1	butyrate/propionate					1913:1931	sodium butyrate/propionate	1906:1931	sodium butyrate/propionate	1906:1931	Capsaicin-evoked calcium responses were increased in naive dorsal root ganglion neurons incubated with both sodium butyrate/propionate alone and with colonic supernatants derived from postinflammatory mice.
32289500	2	70	theme	transient	275:283	arg1	expression					333:342	rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression	268:342	rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression	268:342	These patients have increased rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression, a key transducer of inflammatory pain.
32289500	2	70	theme	transient	275:283	arg1	transducer					351:360	a key transducer	345:360	a key transducer of inflammatory pain	345:381	These patients have increased rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression, a key transducer of inflammatory pain.
32289500	15	71	theme	Microbial-derived	2100:2116	arg1	SCFAs					2118:2122	Microbial-derived SCFAs	2100:2122	Microbial-derived SCFAs	2100:2122	Microbial-derived SCFAs can sensitize nociceptive neurons and may contribute to the pathogenesis of postinflammatory visceral pain.
32289500	14	72	dep	CONCLUSIONS	2005:2015	arg1	plays					2032:2036	plays	2032:2036	plays a central role in postinflammatory visceral hypersensitivity	2032:2097	CONCLUSIONS The microbiome plays a central role in postinflammatory visceral hypersensitivity.
32289500	9	73	theme	DSS	1320:1322	arg1	mice					1324:1327	Postinflammatory DSS mice	1303:1327	Postinflammatory DSS mice	1303:1327	RESULTS Postinflammatory DSS mice developed visceral and somatic hyperalgesia.
32289500	8	74	theme	mass	1276:1279	arg1	spectrometry					1281:1292	targeted mass spectrometry	1267:1292	targeted mass spectrometry	1267:1292	The microbiome composition was evaluated via 16S rRNA gene variable region V4 amplicon sequencing, whereas fecal short-chain fatty acids (SCFAs) were assessed by using targeted mass spectrometry.
32289500	12	75	theme	fecal	1743:1747	arg1	content					1766:1772	fecal acetate/butyrate content	1743:1772	fecal acetate/butyrate content	1743:1772	Postinflammatory mice exhibited both increased SCFA-producing species and fecal acetate/butyrate content compared with controls.
32289500	7	76	theme	nociception	1086:1096	arg1	imaging					1001:1007	Calcium imaging	993:1007	Calcium imaging of isolated dorsal root ganglia neurons	993:1047	Calcium imaging of isolated dorsal root ganglia neurons was used as an in vitro correlate of nociception.
32289500	7	76	theme	nociception	1086:1096	arg1	correlate					1073:1081	an in vitro correlate	1061:1081	an in vitro correlate of nociception	1061:1096	Calcium imaging of isolated dorsal root ganglia neurons was used as an in vitro correlate of nociception.
32289500	2	77	theme	receptor	316:323	arg1	expression					333:342	rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression	268:342	rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression	268:342	These patients have increased rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression, a key transducer of inflammatory pain.
32289500	2	77	theme	receptor	316:323	arg1	transducer					351:360	a key transducer	345:360	a key transducer of inflammatory pain	345:381	These patients have increased rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression, a key transducer of inflammatory pain.
32289500	7	78	theme	in vitro	1064:1071	arg1	imaging					1001:1007	Calcium imaging	993:1007	Calcium imaging of isolated dorsal root ganglia neurons	993:1047	Calcium imaging of isolated dorsal root ganglia neurons was used as an in vitro correlate of nociception.
32289500	7	78	theme	in vitro	1064:1071	arg1	correlate					1073:1081	an in vitro correlate	1061:1081	an in vitro correlate of nociception	1061:1096	Calcium imaging of isolated dorsal root ganglia neurons was used as an in vitro correlate of nociception.
32289500	14	79	theme	visceral	2073:2080	arg1	hypersensitivity					2082:2097	postinflammatory visceral hypersensitivity	2056:2097	postinflammatory visceral hypersensitivity	2056:2097	CONCLUSIONS The microbiome plays a central role in postinflammatory visceral hypersensitivity.
32289500	5	80	theme	microbial	797:805	arg1	transplant					807:816	fecal microbial transplant	791:816	antibiotics as well as fecal microbial transplant (FMT)	768:822	The microbiome was perturbed by using antibiotics as well as fecal microbial transplant (FMT).
32289500	5	80	theme	microbial	797:805	arg1	FMT					819:821	FMT	819:821	FMT	819:821	The microbiome was perturbed by using antibiotics as well as fecal microbial transplant (FMT).
32289500	2	81	theme	potential	294:302	arg1	expression					333:342	rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression	268:342	rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression	268:342	These patients have increased rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression, a key transducer of inflammatory pain.
32289500	2	81	theme	potential	294:302	arg1	transducer					351:360	a key transducer	345:360	a key transducer of inflammatory pain	345:381	These patients have increased rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression, a key transducer of inflammatory pain.
32289500	12	82	theme	increased	1706:1714	arg1	species					1731:1737	increased SCFA-producing species	1706:1737	increased SCFA-producing species	1706:1737	Postinflammatory mice exhibited both increased SCFA-producing species and fecal acetate/butyrate content compared with controls.
32289500	1	83	theme	bowel	180:184	arg1	disease					186:192	inflammatory bowel disease	167:192	inflammatory bowel disease patients	167:201	BACKGROUND & AIMS Despite achieving endoscopic remission, more than 20% of inflammatory bowel disease patients experience chronic abdominal pain.
32289500	15	84	theme	pain	2226:2229	arg1	pathogenesis					2184:2195	the pathogenesis	2180:2195	the pathogenesis of postinflammatory visceral pain	2180:2229	Microbial-derived SCFAs can sensitize nociceptive neurons and may contribute to the pathogenesis of postinflammatory visceral pain.
32289500	11	85	theme	stool	1620:1624	arg1	FMT					1605:1607	FMT	1605:1607	FMT of control stool	1605:1624	FMT of postinflammatory DSS stool into antibiotic-treated mice increased visceral hypersensitivity, whereas FMT of control stool reversed antibiotics' sensitizing effects.
32289500	8	86	theme	microbiome	1103:1112	arg1	composition					1114:1124	The microbiome composition	1099:1124	The microbiome composition	1099:1124	The microbiome composition was evaluated via 16S rRNA gene variable region V4 amplicon sequencing, whereas fecal short-chain fatty acids (SCFAs) were assessed by using targeted mass spectrometry.
32289500	7	87	theme	ganglia	1033:1039	arg1	neurons					1041:1047	isolated dorsal root ganglia neurons	1012:1047	isolated dorsal root ganglia neurons	1012:1047	Calcium imaging of isolated dorsal root ganglia neurons was used as an in vitro correlate of nociception.
32289500	1	88	theme	BACKGROUND	92:101	arg1	AIMS					105:108	BACKGROUND & AIMS	92:108	BACKGROUND & AIMS Despite achieving endoscopic remission, more than 20% of inflammatory bowel disease patients experience chronic abdominal pain.	92:236	BACKGROUND & AIMS Despite achieving endoscopic remission, more than 20% of inflammatory bowel disease patients experience chronic abdominal pain.
32289500	7	89	used	used	1053:1056	arg2	correlate					1073:1081	an in vitro correlate	1061:1081	an in vitro correlate of nociception	1061:1096	Calcium imaging of isolated dorsal root ganglia neurons was used as an in vitro correlate of nociception.
32289500	7	89	used	used	1053:1056	arg2	imaging					1001:1007	Calcium imaging	993:1007	Calcium imaging of isolated dorsal root ganglia neurons	993:1047	Calcium imaging of isolated dorsal root ganglia neurons was used as an in vitro correlate of nociception.
32289500	12	90	theme	Postinflammatory	1669:1684	arg1	mice					1686:1689	Postinflammatory mice	1669:1689	Postinflammatory mice	1669:1689	Postinflammatory mice exhibited both increased SCFA-producing species and fecal acetate/butyrate content compared with controls.
32289500	3	91	theme	disease	411:417	arg1	patients					419:426	inflammatory bowel disease patients	392:426	inflammatory bowel disease patients in remission	392:439	Because inflammatory bowel disease patients in remission exhibit dysbiosis and microbial manipulation alters TRPV1 function, our goal was to examine whether microbial perturbation modulated transient receptor potential function in a mouse model.
32289500	15	92	theme	postinflammatory	2200:2215	arg1	pain					2226:2229	postinflammatory visceral pain	2200:2229	postinflammatory visceral pain	2200:2229	Microbial-derived SCFAs can sensitize nociceptive neurons and may contribute to the pathogenesis of postinflammatory visceral pain.
32289500	10	93	theme	Antibiotic	1374:1383	arg1	administration					1385:1398	Antibiotic administration	1374:1398	Antibiotic administration during DSS recovery	1374:1418	Antibiotic administration during DSS recovery induced visceral, but not somatic, hyperalgesia independent of inflammation.
32289500	11	94	theme	antibiotic-treated	1536:1553	arg1	mice					1555:1558	antibiotic-treated mice	1536:1558	antibiotic-treated mice	1536:1558	FMT of postinflammatory DSS stool into antibiotic-treated mice increased visceral hypersensitivity, whereas FMT of control stool reversed antibiotics' sensitizing effects.
32289500	7	95	theme	dorsal	1021:1026	arg1	neurons					1041:1047	isolated dorsal root ganglia neurons	1012:1047	isolated dorsal root ganglia neurons	1012:1047	Calcium imaging of isolated dorsal root ganglia neurons was used as an in vitro correlate of nociception.
32289500	3	96	theme	inflammatory	392:403	arg1	disease					411:417	inflammatory bowel disease	392:417	inflammatory bowel disease patients in remission	392:439	Because inflammatory bowel disease patients in remission exhibit dysbiosis and microbial manipulation alters TRPV1 function, our goal was to examine whether microbial perturbation modulated transient receptor potential function in a mouse model.
32289500	13	97	theme	dorsal	1857:1862	arg1	neurons					1878:1884	naive dorsal root ganglion neurons	1851:1884	naive dorsal root ganglion neurons incubated with both sodium butyrate/propionate alone and with colonic supernatants derived from postinflammatory mice	1851:2002	Capsaicin-evoked calcium responses were increased in naive dorsal root ganglion neurons incubated with both sodium butyrate/propionate alone and with colonic supernatants derived from postinflammatory mice.
32289500	11	98	theme	stool	1525:1529	arg1	FMT					1497:1499	FMT	1497:1499	FMT of postinflammatory DSS stool into antibiotic-treated mice	1497:1558	FMT of postinflammatory DSS stool into antibiotic-treated mice increased visceral hypersensitivity, whereas FMT of control stool reversed antibiotics' sensitizing effects.
32289500	0	99	theme	Microbial	16:24	arg1	Perturbation					26:37	Colitis-Induced Microbial Perturbation	0:37	Colitis-Induced Microbial Perturbation	0:37	Colitis-Induced Microbial Perturbation Promotes Postinflammatory Visceral Hypersensitivity.
32289500	13	100	theme	ganglion	1869:1876	arg1	neurons					1878:1884	naive dorsal root ganglion neurons	1851:1884	naive dorsal root ganglion neurons incubated with both sodium butyrate/propionate alone and with colonic supernatants derived from postinflammatory mice	1851:2002	Capsaicin-evoked calcium responses were increased in naive dorsal root ganglion neurons incubated with both sodium butyrate/propionate alone and with colonic supernatants derived from postinflammatory mice.
32289500	3	101	theme	TRPV1	493:497	arg1	function					499:506	TRPV1 function	493:506	TRPV1 function	493:506	Because inflammatory bowel disease patients in remission exhibit dysbiosis and microbial manipulation alters TRPV1 function, our goal was to examine whether microbial perturbation modulated transient receptor potential function in a mouse model.
32289500	8	102	theme	rRNA	1148:1151	arg1	sequencing					1186:1195	rRNA gene variable region V4 amplicon sequencing	1148:1195	rRNA gene variable region V4 amplicon sequencing	1148:1195	The microbiome composition was evaluated via 16S rRNA gene variable region V4 amplicon sequencing, whereas fecal short-chain fatty acids (SCFAs) were assessed by using targeted mass spectrometry.
32289500	7	103	theme	Calcium	993:999	arg1	imaging					1001:1007	Calcium imaging	993:1007	Calcium imaging of isolated dorsal root ganglia neurons	993:1047	Calcium imaging of isolated dorsal root ganglia neurons was used as an in vitro correlate of nociception.
32289500	7	103	theme	Calcium	993:999	arg1	correlate					1073:1081	an in vitro correlate	1061:1081	an in vitro correlate of nociception	1061:1096	Calcium imaging of isolated dorsal root ganglia neurons was used as an in vitro correlate of nociception.
32289500	0	104	theme	Visceral	65:72	arg1	Hypersensitivity					74:89	Postinflammatory Visceral Hypersensitivity	48:89	Postinflammatory Visceral Hypersensitivity	48:89	Colitis-Induced Microbial Perturbation Promotes Postinflammatory Visceral Hypersensitivity.
32289500	8	105	theme	variable	1158:1165	arg1	sequencing					1186:1195	rRNA gene variable region V4 amplicon sequencing	1148:1195	rRNA gene variable region V4 amplicon sequencing	1148:1195	The microbiome composition was evaluated via 16S rRNA gene variable region V4 amplicon sequencing, whereas fecal short-chain fatty acids (SCFAs) were assessed by using targeted mass spectrometry.
32289500	8	106	theme	V4	1174:1175	arg1	sequencing					1186:1195	rRNA gene variable region V4 amplicon sequencing	1148:1195	rRNA gene variable region V4 amplicon sequencing	1148:1195	The microbiome composition was evaluated via 16S rRNA gene variable region V4 amplicon sequencing, whereas fecal short-chain fatty acids (SCFAs) were assessed by using targeted mass spectrometry.
32289500	13	107	attach	derived	1969:1975	arg1	mice					1999:2002	postinflammatory mice	1982:2002	postinflammatory mice	1982:2002	Capsaicin-evoked calcium responses were increased in naive dorsal root ganglion neurons incubated with both sodium butyrate/propionate alone and with colonic supernatants derived from postinflammatory mice.
32289500	13	107	attach	derived	1969:1975	arg2	supernatants					1956:1967	colonic supernatants	1948:1967	colonic supernatants derived from postinflammatory mice	1948:2002	Capsaicin-evoked calcium responses were increased in naive dorsal root ganglion neurons incubated with both sodium butyrate/propionate alone and with colonic supernatants derived from postinflammatory mice.
32289500	10	108	theme	visceral	1428:1435	arg1	hyperalgesia					1455:1466	visceral, but not somatic, hyperalgesia	1428:1466	visceral, but not somatic, hyperalgesia independent of inflammation	1428:1494	Antibiotic administration during DSS recovery induced visceral, but not somatic, hyperalgesia independent of inflammation.
32289500	2	109	theme	pain	378:381	arg1	expression					333:342	rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression	268:342	rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression	268:342	These patients have increased rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression, a key transducer of inflammatory pain.
32289500	2	109	theme	pain	378:381	arg1	transducer					351:360	a key transducer	345:360	a key transducer of inflammatory pain	345:381	These patients have increased rectal transient receptor potential vanilloid-1 receptor (TRPV1) expression, a key transducer of inflammatory pain.
32289500	6	110	theme	visceromotor	885:896	arg1	responses					898:906	visceromotor responses	885:906	visceromotor responses to colorectal distention	885:931	Visceral and somatic sensitivity were assessed by recording visceromotor responses to colorectal distention and using hot plate/automated Von Frey tests, respectively.
32289500	13	111	theme	calcium	1815:1821	arg1	responses					1823:1831	Capsaicin-evoked calcium responses	1798:1831	Capsaicin-evoked calcium responses	1798:1831	Capsaicin-evoked calcium responses were increased in naive dorsal root ganglion neurons incubated with both sodium butyrate/propionate alone and with colonic supernatants derived from postinflammatory mice.
32289500	8	112	dep	evaluated	1130:1138	arg1	whereas					1198:1204	whereas	1198:1204	whereas	1198:1204	The microbiome composition was evaluated via 16S rRNA gene variable region V4 amplicon sequencing, whereas fecal short-chain fatty acids (SCFAs) were assessed by using targeted mass spectrometry.
32289500	10	113	theme	somatic	1446:1452	arg1	hyperalgesia					1455:1466	visceral, but not somatic, hyperalgesia	1428:1466	visceral, but not somatic, hyperalgesia independent of inflammation	1428:1494	Antibiotic administration during DSS recovery induced visceral, but not somatic, hyperalgesia independent of inflammation.
32289500	3	114	theme	potential	593:601	arg1	function					603:610	transient receptor potential function	574:610	transient receptor potential function	574:610	Because inflammatory bowel disease patients in remission exhibit dysbiosis and microbial manipulation alters TRPV1 function, our goal was to examine whether microbial perturbation modulated transient receptor potential function in a mouse model.
34360993	8	0	theme	canonical	1168:1176	arg1	sites					1199:1203	the canonical four N-glycosylation sites	1164:1203	the canonical four N-glycosylation sites of human CP	1164:1215	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	8	0	theme	canonical	1168:1176	arg1	CP					1214:1215	human CP	1208:1215	human CP	1208:1215	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	8	1	theme	predominant	1062:1072	arg1	glycoforms					1074:1083	predominant glycoforms	1062:1083	predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11	1062:1112	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	8	1	theme	predominant	1062:1072	arg1	HexNAc2Hex11					1101:1112	HexNAc2Hex11	1101:1112	HexNAc2Hex11	1101:1112	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	8	1	theme	predominant	1062:1072	arg1	HexNAc2Hex8					1085:1095	HexNAc2Hex8	1085:1095	HexNAc2Hex8	1085:1095	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	7	2	theme	two-step	998:1005	arg1	procedure					1020:1028	an improved two-step purification procedure	986:1028	an improved two-step purification procedure	986:1028	Highly pure biologically active protein has been obtained by an improved two-step purification procedure.
34360993	3	3	theme	structural	466:475	arg1	studies					492:498	structural and functional studies	466:498	structural and functional studies	466:498	Many aspects of the (patho)physiology of CP are still unclear and would benefit from the availability of recombinant protein for structural and functional studies.
34360993	4	4	theme	enzyme	552:557	arg1	therapy					571:577	enzyme replacement therapy	552:577	enzyme replacement therapy for the treatment of aceruloplasminemia	552:617	Furthermore, recombinant CP could be evaluated for enzyme replacement therapy for the treatment of aceruloplasminemia.
34360993	9	5	from	advancement	1297:1307	arg1	field					1316:1320	the field	1312:1320	the field of CP biology	1312:1334	The availability of high-quality recombinant human CP represents a significant advancement in the field of CP biology.
34360993	9	6	theme	CP	1325:1326	arg1	biology					1328:1334	CP biology	1325:1334	CP biology	1325:1334	The availability of high-quality recombinant human CP represents a significant advancement in the field of CP biology.
34360993	1	7	from	homeostasis	142:152	arg1	vertebrates					157:167	vertebrates	157:167	vertebrates together with the iron exporter ferroportin	157:211	The ferroxidase ceruloplasmin (CP) plays a crucial role in iron homeostasis in vertebrates together with the iron exporter ferroportin.
34360993	10	8	theme	further	1385:1391	arg1	glycoengineering					1401:1416	further careful glycoengineering	1385:1416	further careful glycoengineering of the SM5 strain	1385:1434	However, productivity needs to be increased and further careful glycoengineering of the SM5 strain is mandatory in order to evaluate the possible therapeutic use of the recombinant protein for enzyme replacement therapy of aceruloplasminemia patients.
34360993	5	9	theme	Pichia	734:739	arg1	pastoris					741:748	glycoengineered Pichia pastoris SuperMan5	718:758	glycoengineered Pichia pastoris SuperMan5	718:758	We report the production and preliminary characterization of high-quality recombinant human CP in glycoengineered Pichia pastoris SuperMan5.
34360993	6	10	theme	CP	921:922	arg1	isoform					904:910	the secreted isoform	891:910	the secreted isoform of human CP	891:922	A modified yeast strain lacking the endogenous ferroxidase has been generated and employed as host for heterologous expression of the secreted isoform of human CP.
34360993	10	11	theme	recombinant	1506:1516	arg1	protein					1518:1524	the recombinant protein	1502:1524	the recombinant protein	1502:1524	However, productivity needs to be increased and further careful glycoengineering of the SM5 strain is mandatory in order to evaluate the possible therapeutic use of the recombinant protein for enzyme replacement therapy of aceruloplasminemia patients.
34360993	10	12	theme	SM5	1425:1427	arg1	strain					1429:1434	the SM5 strain	1421:1434	the SM5 strain	1421:1434	However, productivity needs to be increased and further careful glycoengineering of the SM5 strain is mandatory in order to evaluate the possible therapeutic use of the recombinant protein for enzyme replacement therapy of aceruloplasminemia patients.
34360993	2	13	theme	rare	274:277	arg1	disease					297:303	a rare neurodegenerative disease	272:303	a rare neurodegenerative disease for which no cure is available	272:334	Mutations in the CP gene give rise to aceruloplasminemia, a rare neurodegenerative disease for which no cure is available.
34360993	2	13	theme	rare	274:277	arg1	aceruloplasminemia					252:269	aceruloplasminemia	252:269	aceruloplasminemia	252:269	Mutations in the CP gene give rise to aceruloplasminemia, a rare neurodegenerative disease for which no cure is available.
34360993	8	14	dep	glycoforms	1074:1083	arg1	glycoforms					1074:1083	predominant glycoforms	1062:1083	predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11	1062:1112	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	8	14	dep	glycoforms	1074:1083	arg1	HexNAc2Hex11					1101:1112	HexNAc2Hex11	1101:1112	HexNAc2Hex11	1101:1112	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	8	14	dep	glycoforms	1074:1083	arg1	HexNAc2Hex8					1085:1095	HexNAc2Hex8	1085:1095	HexNAc2Hex8	1085:1095	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	6	15	theme	human	915:919	arg1	CP					921:922	human CP	915:922	human CP	915:922	A modified yeast strain lacking the endogenous ferroxidase has been generated and employed as host for heterologous expression of the secreted isoform of human CP.
34360993	5	16	theme	preliminary	649:659	arg1	characterization					661:676	preliminary characterization	649:676	preliminary characterization	649:676	We report the production and preliminary characterization of high-quality recombinant human CP in glycoengineered Pichia pastoris SuperMan5.
34360993	9	17	theme	recombinant	1251:1261	arg1	CP					1269:1270	high-quality recombinant human CP	1238:1270	high-quality recombinant human CP	1238:1270	The availability of high-quality recombinant human CP represents a significant advancement in the field of CP biology.
34360993	4	18	theme	replacement	559:569	arg1	therapy					571:577	enzyme replacement therapy	552:577	enzyme replacement therapy for the treatment of aceruloplasminemia	552:617	Furthermore, recombinant CP could be evaluated for enzyme replacement therapy for the treatment of aceruloplasminemia.
34360993	4	19	theme	aceruloplasminemia	600:617	arg1	treatment					587:595	the treatment	583:595	the treatment of aceruloplasminemia	583:617	Furthermore, recombinant CP could be evaluated for enzyme replacement therapy for the treatment of aceruloplasminemia.
34360993	2	20	from	Mutations	214:222	arg1	gene					234:237	the CP gene	227:237	the CP gene	227:237	Mutations in the CP gene give rise to aceruloplasminemia, a rare neurodegenerative disease for which no cure is available.
34360993	3	21	theme	Many	337:340	arg1	aspects					342:348	Many aspects	337:348	Many aspects of the (patho)physiology of CP	337:379	Many aspects of the (patho)physiology of CP are still unclear and would benefit from the availability of recombinant protein for structural and functional studies.
34360993	6	22	theme	isoform	904:910	arg1	expression					877:886	heterologous expression	864:886	heterologous expression of the secreted isoform of human CP	864:922	A modified yeast strain lacking the endogenous ferroxidase has been generated and employed as host for heterologous expression of the secreted isoform of human CP.
34360993	8	23	theme	Glycan	1031:1036	arg1	analysis					1038:1045	Glycan analysis	1031:1045	Glycan analysis	1031:1045	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	1	24	theme	ferroxidase	82:92	arg1	CP					109:110	CP	109:110	CP	109:110	The ferroxidase ceruloplasmin (CP) plays a crucial role in iron homeostasis in vertebrates together with the iron exporter ferroportin.
34360993	1	24	theme	ferroxidase	82:92	arg1	ceruloplasmin					94:106	The ferroxidase ceruloplasmin	78:106	The ferroxidase ceruloplasmin (CP)	78:111	The ferroxidase ceruloplasmin (CP) plays a crucial role in iron homeostasis in vertebrates together with the iron exporter ferroportin.
34360993	3	25	theme	patho	358:362	arg1	physiology					364:373	the (patho)physiology	353:373	the (patho)physiology of CP	353:379	Many aspects of the (patho)physiology of CP are still unclear and would benefit from the availability of recombinant protein for structural and functional studies.
34360993	6	26	theme	secreted	895:902	arg1	isoform					904:910	the secreted isoform	891:910	the secreted isoform of human CP	891:922	A modified yeast strain lacking the endogenous ferroxidase has been generated and employed as host for heterologous expression of the secreted isoform of human CP.
34360993	10	27	theme	possible	1474:1481	arg1	use					1495:1497	the possible therapeutic use	1470:1497	the possible therapeutic use of the recombinant protein for enzyme replacement therapy of aceruloplasminemia patients	1470:1586	However, productivity needs to be increased and further careful glycoengineering of the SM5 strain is mandatory in order to evaluate the possible therapeutic use of the recombinant protein for enzyme replacement therapy of aceruloplasminemia patients.
34360993	6	28	theme	endogenous	797:806	arg1	ferroxidase					808:818	the endogenous ferroxidase	793:818	the endogenous ferroxidase	793:818	A modified yeast strain lacking the endogenous ferroxidase has been generated and employed as host for heterologous expression of the secreted isoform of human CP.
34360993	8	29	theme	human	1208:1212	arg1	CP					1214:1215	human CP	1208:1215	human CP	1208:1215	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	10	30	theme	therapeutic	1483:1493	arg1	use					1495:1497	the possible therapeutic use	1470:1497	the possible therapeutic use of the recombinant protein for enzyme replacement therapy of aceruloplasminemia patients	1470:1586	However, productivity needs to be increased and further careful glycoengineering of the SM5 strain is mandatory in order to evaluate the possible therapeutic use of the recombinant protein for enzyme replacement therapy of aceruloplasminemia patients.
34360993	9	31	theme	high-quality	1238:1249	arg1	CP					1269:1270	high-quality recombinant human CP	1238:1270	high-quality recombinant human CP	1238:1270	The availability of high-quality recombinant human CP represents a significant advancement in the field of CP biology.
34360993	0	32	theme	Human	26:30	arg1	Ceruloplasmin					32:44	Recombinant Human Ceruloplasmin	14:44	Recombinant Human Ceruloplasmin	14:44	Production of Recombinant Human Ceruloplasmin: Improvements and Perspectives.
34360993	8	33	theme	sites	1199:1203	arg1	three					1155:1159	three	1155:1159	three	1155:1159	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	8	33	theme	sites	1199:1203	arg1	sites					1199:1203	the canonical four N-glycosylation sites	1164:1203	the canonical four N-glycosylation sites of human CP	1164:1215	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	8	33	theme	sites	1199:1203	arg1	Asn119					1127:1132	Asn119	1127:1132	Asn119	1127:1132	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	8	33	theme	sites	1199:1203	arg1	CP					1214:1215	human CP	1208:1215	human CP	1208:1215	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	5	34	theme	human	706:710	arg1	CP					712:713	high-quality recombinant human CP	681:713	high-quality recombinant human CP	681:713	We report the production and preliminary characterization of high-quality recombinant human CP in glycoengineered Pichia pastoris SuperMan5.
34360993	7	35	dep	pure	932:935	arg1	active					950:955	active	950:955	active	950:955	Highly pure biologically active protein has been obtained by an improved two-step purification procedure.
34360993	10	36	theme	strain	1429:1434	arg1	glycoengineering					1401:1416	further careful glycoengineering	1385:1416	further careful glycoengineering of the SM5 strain	1385:1434	However, productivity needs to be increased and further careful glycoengineering of the SM5 strain is mandatory in order to evaluate the possible therapeutic use of the recombinant protein for enzyme replacement therapy of aceruloplasminemia patients.
34360993	1	37	theme	iron	187:190	arg1	ferroportin					201:211	the iron exporter ferroportin	183:211	the iron exporter ferroportin	183:211	The ferroxidase ceruloplasmin (CP) plays a crucial role in iron homeostasis in vertebrates together with the iron exporter ferroportin.
34360993	0	38	theme	Recombinant	14:24	arg1	Ceruloplasmin					32:44	Recombinant Human Ceruloplasmin	14:44	Recombinant Human Ceruloplasmin	14:44	Production of Recombinant Human Ceruloplasmin: Improvements and Perspectives.
34360993	5	39	theme	CP	712:713	arg1	production					634:643	production	634:643	production	634:643	We report the production and preliminary characterization of high-quality recombinant human CP in glycoengineered Pichia pastoris SuperMan5.
34360993	5	39	theme	CP	712:713	arg1	characterization					661:676	preliminary characterization	649:676	preliminary characterization	649:676	We report the production and preliminary characterization of high-quality recombinant human CP in glycoengineered Pichia pastoris SuperMan5.
34360993	9	40	theme	human	1263:1267	arg1	CP					1269:1270	high-quality recombinant human CP	1238:1270	high-quality recombinant human CP	1238:1270	The availability of high-quality recombinant human CP represents a significant advancement in the field of CP biology.
34360993	2	41	theme	neurodegenerative	279:295	arg1	disease					297:303	a rare neurodegenerative disease	272:303	a rare neurodegenerative disease for which no cure is available	272:334	Mutations in the CP gene give rise to aceruloplasminemia, a rare neurodegenerative disease for which no cure is available.
34360993	2	41	theme	neurodegenerative	279:295	arg1	aceruloplasminemia					252:269	aceruloplasminemia	252:269	aceruloplasminemia	252:269	Mutations in the CP gene give rise to aceruloplasminemia, a rare neurodegenerative disease for which no cure is available.
34360993	1	42	theme	exporter	192:199	arg1	ferroportin					201:211	the iron exporter ferroportin	183:211	the iron exporter ferroportin	183:211	The ferroxidase ceruloplasmin (CP) plays a crucial role in iron homeostasis in vertebrates together with the iron exporter ferroportin.
34360993	3	43	theme	CP	378:379	arg1	physiology					364:373	the (patho)physiology	353:373	the (patho)physiology of CP	353:379	Many aspects of the (patho)physiology of CP are still unclear and would benefit from the availability of recombinant protein for structural and functional studies.
34360993	10	44	theme	protein	1518:1524	arg1	use					1495:1497	the possible therapeutic use	1470:1497	the possible therapeutic use of the recombinant protein for enzyme replacement therapy of aceruloplasminemia patients	1470:1586	However, productivity needs to be increased and further careful glycoengineering of the SM5 strain is mandatory in order to evaluate the possible therapeutic use of the recombinant protein for enzyme replacement therapy of aceruloplasminemia patients.
34360993	9	45	theme	CP	1269:1270	arg1	availability					1222:1233	The availability	1218:1233	The availability of high-quality recombinant human CP	1218:1270	The availability of high-quality recombinant human CP represents a significant advancement in the field of CP biology.
34360993	2	46	theme	CP	231:232	arg1	gene					234:237	the CP gene	227:237	the CP gene	227:237	Mutations in the CP gene give rise to aceruloplasminemia, a rare neurodegenerative disease for which no cure is available.
34360993	0	47	theme	Ceruloplasmin	32:44	arg1	Production					0:9	Production	0:9	Production of Recombinant Human Ceruloplasmin	0:44	Production of Recombinant Human Ceruloplasmin: Improvements and Perspectives.
34360993	5	48	theme	glycoengineered	718:732	arg1	pastoris					741:748	glycoengineered Pichia pastoris SuperMan5	718:758	glycoengineered Pichia pastoris SuperMan5	718:758	We report the production and preliminary characterization of high-quality recombinant human CP in glycoengineered Pichia pastoris SuperMan5.
34360993	10	49	theme	patients	1579:1586	arg1	therapy					1549:1555	enzyme replacement therapy	1530:1555	enzyme replacement therapy of aceruloplasminemia patients	1530:1586	However, productivity needs to be increased and further careful glycoengineering of the SM5 strain is mandatory in order to evaluate the possible therapeutic use of the recombinant protein for enzyme replacement therapy of aceruloplasminemia patients.
34360993	0	50	dep	Improvements	47:58	arg1	Production					0:9	Production	0:9	Production of Recombinant Human Ceruloplasmin	0:44	Production of Recombinant Human Ceruloplasmin: Improvements and Perspectives.
34360993	8	51	located	found	1118:1122	arg2	HexNAc2Hex11					1101:1112	HexNAc2Hex11	1101:1112	HexNAc2Hex11	1101:1112	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	8	51	located	found	1118:1122	arg1	Asn743					1147:1152	Asn743	1147:1152	Asn743	1147:1152	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	8	51	located	found	1118:1122	arg1	sites					1199:1203	the canonical four N-glycosylation sites	1164:1203	the canonical four N-glycosylation sites of human CP	1164:1215	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	8	51	located	found	1118:1122	arg1	Asn119					1127:1132	Asn119	1127:1132	Asn119	1127:1132	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	8	51	located	found	1118:1122	arg1	three					1155:1159	three	1155:1159	three	1155:1159	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	8	51	located	found	1118:1122	arg1	Asn378					1135:1140	Asn378	1135:1140	Asn378	1135:1140	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	8	51	located	found	1118:1122	arg2	HexNAc2Hex8					1085:1095	HexNAc2Hex8	1085:1095	HexNAc2Hex8	1085:1095	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	8	51	located	found	1118:1122	arg2	glycoforms					1074:1083	predominant glycoforms	1062:1083	predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11	1062:1112	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	3	52	theme	physiology	364:373	arg1	aspects					342:348	Many aspects	337:348	Many aspects of the (patho)physiology of CP	337:379	Many aspects of the (patho)physiology of CP are still unclear and would benefit from the availability of recombinant protein for structural and functional studies.
34360993	8	53	theme	N-glycosylation	1183:1197	arg1	sites					1199:1203	the canonical four N-glycosylation sites	1164:1203	the canonical four N-glycosylation sites of human CP	1164:1215	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	8	53	theme	N-glycosylation	1183:1197	arg1	CP					1214:1215	human CP	1208:1215	human CP	1208:1215	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	10	54	theme	aceruloplasminemia	1560:1577	arg1	patients					1579:1586	aceruloplasminemia patients	1560:1586	aceruloplasminemia patients	1560:1586	However, productivity needs to be increased and further careful glycoengineering of the SM5 strain is mandatory in order to evaluate the possible therapeutic use of the recombinant protein for enzyme replacement therapy of aceruloplasminemia patients.
34360993	6	55	theme	heterologous	864:875	arg1	expression					877:886	heterologous expression	864:886	heterologous expression of the secreted isoform of human CP	864:922	A modified yeast strain lacking the endogenous ferroxidase has been generated and employed as host for heterologous expression of the secreted isoform of human CP.
34360993	6	56	theme	yeast	772:776	arg1	host					855:858	host	855:858	host	855:858	A modified yeast strain lacking the endogenous ferroxidase has been generated and employed as host for heterologous expression of the secreted isoform of human CP.
34360993	6	56	theme	yeast	772:776	arg1	strain					778:783	A modified yeast strain	761:783	A modified yeast strain lacking the endogenous ferroxidase	761:818	A modified yeast strain lacking the endogenous ferroxidase has been generated and employed as host for heterologous expression of the secreted isoform of human CP.
34360993	3	57	theme	protein	454:460	arg1	availability					426:437	the availability	422:437	the availability of recombinant protein for structural and functional studies	422:498	Many aspects of the (patho)physiology of CP are still unclear and would benefit from the availability of recombinant protein for structural and functional studies.
34360993	9	58	theme	biology	1328:1334	arg1	field					1316:1320	the field	1312:1320	the field of CP biology	1312:1334	The availability of high-quality recombinant human CP represents a significant advancement in the field of CP biology.
34360993	4	59	theme	recombinant	514:524	arg1	CP					526:527	recombinant CP	514:527	recombinant CP	514:527	Furthermore, recombinant CP could be evaluated for enzyme replacement therapy for the treatment of aceruloplasminemia.
34360993	10	60	theme	enzyme	1530:1535	arg1	therapy					1549:1555	enzyme replacement therapy	1530:1555	enzyme replacement therapy of aceruloplasminemia patients	1530:1586	However, productivity needs to be increased and further careful glycoengineering of the SM5 strain is mandatory in order to evaluate the possible therapeutic use of the recombinant protein for enzyme replacement therapy of aceruloplasminemia patients.
34360993	9	61	theme	significant	1285:1295	arg1	advancement					1297:1307	a significant advancement	1283:1307	a significant advancement in the field of CP biology	1283:1334	The availability of high-quality recombinant human CP represents a significant advancement in the field of CP biology.
34360993	6	62	theme	modified	763:770	arg1	host					855:858	host	855:858	host	855:858	A modified yeast strain lacking the endogenous ferroxidase has been generated and employed as host for heterologous expression of the secreted isoform of human CP.
34360993	6	62	theme	modified	763:770	arg1	strain					778:783	A modified yeast strain	761:783	A modified yeast strain lacking the endogenous ferroxidase	761:818	A modified yeast strain lacking the endogenous ferroxidase has been generated and employed as host for heterologous expression of the secreted isoform of human CP.
34360993	5	63	from	production	634:643	arg1	pastoris					741:748	glycoengineered Pichia pastoris SuperMan5	718:758	glycoengineered Pichia pastoris SuperMan5	718:758	We report the production and preliminary characterization of high-quality recombinant human CP in glycoengineered Pichia pastoris SuperMan5.
34360993	5	64	theme	high-quality	681:692	arg1	CP					712:713	high-quality recombinant human CP	681:713	high-quality recombinant human CP	681:713	We report the production and preliminary characterization of high-quality recombinant human CP in glycoengineered Pichia pastoris SuperMan5.
34360993	1	65	theme	crucial	121:127	arg1	role					129:132	a crucial role	119:132	a crucial role	119:132	The ferroxidase ceruloplasmin (CP) plays a crucial role in iron homeostasis in vertebrates together with the iron exporter ferroportin.
34360993	7	66	theme	pure	932:935	arg1	protein					957:963	Highly pure biologically active protein	925:963	Highly pure biologically active protein	925:963	Highly pure biologically active protein has been obtained by an improved two-step purification procedure.
34360993	5	67	theme	recombinant	694:704	arg1	CP					712:713	high-quality recombinant human CP	681:713	high-quality recombinant human CP	681:713	We report the production and preliminary characterization of high-quality recombinant human CP in glycoengineered Pichia pastoris SuperMan5.
34360993	7	68	theme	purification	1007:1018	arg1	procedure					1020:1028	an improved two-step purification procedure	986:1028	an improved two-step purification procedure	986:1028	Highly pure biologically active protein has been obtained by an improved two-step purification procedure.
34360993	3	69	theme	recombinant	442:452	arg1	protein					454:460	recombinant protein	442:460	recombinant protein	442:460	Many aspects of the (patho)physiology of CP are still unclear and would benefit from the availability of recombinant protein for structural and functional studies.
34360993	8	70	theme	CP	1214:1215	arg1	sites					1199:1203	the canonical four N-glycosylation sites	1164:1203	the canonical four N-glycosylation sites of human CP	1164:1215	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	8	70	theme	CP	1214:1215	arg1	CP					1214:1215	human CP	1208:1215	human CP	1208:1215	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	10	71	theme	careful	1393:1399	arg1	glycoengineering					1401:1416	further careful glycoengineering	1385:1416	further careful glycoengineering of the SM5 strain	1385:1434	However, productivity needs to be increased and further careful glycoengineering of the SM5 strain is mandatory in order to evaluate the possible therapeutic use of the recombinant protein for enzyme replacement therapy of aceruloplasminemia patients.
34360993	8	72	gly	N-glycosylation	1183:1197	arg2	sites					1199:1203	the canonical four N-glycosylation sites	1164:1203	the canonical four N-glycosylation sites of human CP	1164:1215	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	8	72	gly	N-glycosylation	1183:1197	arg1	CP					1214:1215	human CP	1208:1215	human CP	1208:1215	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	8	72	gly	N-glycosylation	1183:1197	arg2	CP					1214:1215	human CP	1208:1215	human CP	1208:1215	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	8	72	gly	N-glycosylation	1183:1197	arg2	four					1178:1181	four	1178:1181	four	1178:1181	Glycan analysis indicates that predominant glycoforms HexNAc2Hex8 and HexNAc2Hex11 are found at Asn119, Asn378, and Asn743, three of the canonical four N-glycosylation sites of human CP.
34360993	3	73	theme	functional	481:490	arg1	studies					492:498	structural and functional studies	466:498	structural and functional studies	466:498	Many aspects of the (patho)physiology of CP are still unclear and would benefit from the availability of recombinant protein for structural and functional studies.
34360993	5	74	from	characterization	661:676	arg1	pastoris					741:748	glycoengineered Pichia pastoris SuperMan5	718:758	glycoengineered Pichia pastoris SuperMan5	718:758	We report the production and preliminary characterization of high-quality recombinant human CP in glycoengineered Pichia pastoris SuperMan5.
34360993	5	75	dep	production	634:643	arg1	the					630:632	the	630:632	the	630:632	We report the production and preliminary characterization of high-quality recombinant human CP in glycoengineered Pichia pastoris SuperMan5.
34360993	7	76	theme	improved	989:996	arg1	procedure					1020:1028	an improved two-step purification procedure	986:1028	an improved two-step purification procedure	986:1028	Highly pure biologically active protein has been obtained by an improved two-step purification procedure.
34360993	1	77	theme	iron	137:140	arg1	homeostasis					142:152	iron homeostasis	137:152	iron homeostasis in vertebrates together with the iron exporter ferroportin	137:211	The ferroxidase ceruloplasmin (CP) plays a crucial role in iron homeostasis in vertebrates together with the iron exporter ferroportin.
34360993	10	78	theme	replacement	1537:1547	arg1	therapy					1549:1555	enzyme replacement therapy	1530:1555	enzyme replacement therapy of aceruloplasminemia patients	1530:1586	However, productivity needs to be increased and further careful glycoengineering of the SM5 strain is mandatory in order to evaluate the possible therapeutic use of the recombinant protein for enzyme replacement therapy of aceruloplasminemia patients.
32302275	13	0	dep	A.	1429:1430	arg1	monumenti					1432:1440	monumenti	1432:1440	monumenti	1432:1440	The digital DNA-DNA hybridization (GGDC) and average nucleotide identity (ANI) values between strain F435T and A. monumenti MON 2.2T were 24.6 and 81.8 %, respectively.
32302275	9	1	theme	major	1039:1043	arg1	acid					1051:1054	The major fatty acid	1035:1054	The major fatty acid	1035:1054	The major fatty acid was determined to be anteiso-C15 : 0.
32302275	9	1	theme	major	1039:1043	arg1	 0					1090:1091	 0	1090:1091	 0	1090:1091	The major fatty acid was determined to be anteiso-C15 : 0.
32302275	14	2	theme	strain	1579:1584	arg1	F435T					1586:1590	strain F435T	1579:1590	strain F435T	1579:1590	Based on the results of phenotypic, chemotaxonomic, phylogenetic and phylogenomic analyses, strain F435T represents a novel specie of the genus Auraticoccus, for which the name Auraticoccus cholistanensis sp.
32302275	12	3	theme	G+C	1277:1279	arg1	%					1315:1315	73.5 mol%	1307:1315	73.5 mol%	1307:1315	The genomic G+C content was calculated as 73.5 mol%.
32302275	12	3	theme	G+C	1277:1279	arg1	content					1281:1287	The genomic G+C content	1265:1287	The genomic G+C content	1265:1287	The genomic G+C content was calculated as 73.5 mol%.
32302275	6	4	dep	Desertihabitans	798:812	arg1	aurantiacus					814:824	aurantiacus	814:824	aurantiacus	814:824	Results of blast analysis based on 16S rRNA gene sequences showed that Auraticoccus monumenti MON 2.2T was its closest relative with 97.4 % similarity followed by Desertihabitans aurantiacus CPCC 204711T (95.2 %).
32302275	6	5	theme	gene	679:682	arg1	sequences					684:692	16S rRNA gene sequences	670:692	16S rRNA gene sequences	670:692	Results of blast analysis based on 16S rRNA gene sequences showed that Auraticoccus monumenti MON 2.2T was its closest relative with 97.4 % similarity followed by Desertihabitans aurantiacus CPCC 204711T (95.2 %).
32302275	14	6	theme	analyses	1569:1576	arg1	results					1500:1506	the results	1496:1506	the results of phenotypic, chemotaxonomic, phylogenetic and phylogenomic analyses	1496:1576	Based on the results of phenotypic, chemotaxonomic, phylogenetic and phylogenomic analyses, strain F435T represents a novel specie of the genus Auraticoccus, for which the name Auraticoccus cholistanensis sp.
32302275	6	7	theme	97.4 	768:772	arg1	%					773:773	%	773:773	%	773:773	Results of blast analysis based on 16S rRNA gene sequences showed that Auraticoccus monumenti MON 2.2T was its closest relative with 97.4 % similarity followed by Desertihabitans aurantiacus CPCC 204711T (95.2 %).
32302275	6	8	theme	16S	670:672	arg1	sequences					684:692	16S rRNA gene sequences	670:692	16S rRNA gene sequences	670:692	Results of blast analysis based on 16S rRNA gene sequences showed that Auraticoccus monumenti MON 2.2T was its closest relative with 97.4 % similarity followed by Desertihabitans aurantiacus CPCC 204711T (95.2 %).
32302275	12	9	theme	73.5 mol	1307:1314	arg1	%					1315:1315	73.5 mol%	1307:1315	73.5 mol%	1307:1315	The genomic G+C content was calculated as 73.5 mol%.
32302275	12	9	theme	73.5 mol	1307:1314	arg1	content					1281:1287	The genomic G+C content	1265:1287	The genomic G+C content	1265:1287	The genomic G+C content was calculated as 73.5 mol%.
32302275	11	10	theme	predominant	1227:1237	arg1	MK-9					1255:1258	MK-9	1255:1258	MK-9(H4)	1255:1262	The predominant menaquinone was MK-9(H4).
32302275	11	10	theme	predominant	1227:1237	arg1	menaquinone					1239:1249	The predominant menaquinone	1223:1249	The predominant menaquinone	1223:1249	The predominant menaquinone was MK-9(H4).
32302275	7	11	theme	Auraticoccus	943:954	arg1	member					923:928	the only member	914:928	the only member of the genus Auraticoccus	914:954	In phylogenetic trees, strain F435T formed a robust cluster with the only member of the genus Auraticoccus.
32302275	16	12	theme	type	1718:1721	arg1	F435T					1733:1737	F435T	1733:1737	F435T (=JCM 33648T=CGMCC 1.17443T)	1733:1766	The type strain is F435T (=JCM 33648T=CGMCC 1.17443T).
32302275	16	12	theme	type	1718:1721	arg1	strain					1723:1728	The type strain	1714:1728	The type strain	1714:1728	The type strain is F435T (=JCM 33648T=CGMCC 1.17443T).
32302275	5	13	theme	NaCl	614:617	arg1	presence					591:598	the presence	587:598	the presence of 0‒8% (w/v) NaCl (optimum, 0 %)	587:632	The novel strain grew at 15‒37 °C (optimum, 25‒30 °C), pH 7‒11 (optimum, pH 7-8) and in the presence of 0‒8% (w/v) NaCl (optimum, 0 %).
32302275	14	14	theme	phylogenomic	1556:1567	arg1	analyses					1569:1576	phenotypic, chemotaxonomic, phylogenetic and phylogenomic analyses	1511:1576	phenotypic, chemotaxonomic, phylogenetic and phylogenomic analyses	1511:1576	Based on the results of phenotypic, chemotaxonomic, phylogenetic and phylogenomic analyses, strain F435T represents a novel specie of the genus Auraticoccus, for which the name Auraticoccus cholistanensis sp.
32302275	14	15	theme	phylogenetic	1539:1550	arg1	analyses					1569:1576	phenotypic, chemotaxonomic, phylogenetic and phylogenomic analyses	1511:1576	phenotypic, chemotaxonomic, phylogenetic and phylogenomic analyses	1511:1576	Based on the results of phenotypic, chemotaxonomic, phylogenetic and phylogenomic analyses, strain F435T represents a novel specie of the genus Auraticoccus, for which the name Auraticoccus cholistanensis sp.
32302275	13	16	theme	strain	1412:1417	arg1	F435T					1419:1423	strain F435T	1412:1423	strain F435T	1412:1423	The digital DNA-DNA hybridization (GGDC) and average nucleotide identity (ANI) values between strain F435T and A. monumenti MON 2.2T were 24.6 and 81.8 %, respectively.
32302275	5	17	theme	%	606:606	arg1	NaCl					614:617	0‒8% (w/v) NaCl	603:617	0‒8% (w/v) NaCl (optimum, 0 %)	603:632	The novel strain grew at 15‒37 °C (optimum, 25‒30 °C), pH 7‒11 (optimum, pH 7-8) and in the presence of 0‒8% (w/v) NaCl (optimum, 0 %).
32302275	5	17	theme	%	606:606	arg1	optimum					620:626	optimum	620:626	optimum	620:626	The novel strain grew at 15‒37 °C (optimum, 25‒30 °C), pH 7‒11 (optimum, pH 7-8) and in the presence of 0‒8% (w/v) NaCl (optimum, 0 %).
32302275	14	18	theme	chemotaxonomic	1523:1536	arg1	analyses					1569:1576	phenotypic, chemotaxonomic, phylogenetic and phylogenomic analyses	1511:1576	phenotypic, chemotaxonomic, phylogenetic and phylogenomic analyses	1511:1576	Based on the results of phenotypic, chemotaxonomic, phylogenetic and phylogenomic analyses, strain F435T represents a novel specie of the genus Auraticoccus, for which the name Auraticoccus cholistanensis sp.
32302275	7	19	theme	strain	872:877	arg1	F435T					879:883	strain F435T	872:883	strain F435T	872:883	In phylogenetic trees, strain F435T formed a robust cluster with the only member of the genus Auraticoccus.
32302275	1	20	theme	emended	102:108	arg1	description					110:120	emended description	102:120	emended description of the genus Auraticoccus	102:146	nov., an actinomycete isolated from soil of the Cholistan Desert, and emended description of the genus Auraticoccus.
32302275	5	21	theme	w/v	609:611	arg1	NaCl					614:617	0‒8% (w/v) NaCl	603:617	0‒8% (w/v) NaCl (optimum, 0 %)	603:632	The novel strain grew at 15‒37 °C (optimum, 25‒30 °C), pH 7‒11 (optimum, pH 7-8) and in the presence of 0‒8% (w/v) NaCl (optimum, 0 %).
32302275	5	21	theme	w/v	609:611	arg1	optimum					620:626	optimum	620:626	optimum	620:626	The novel strain grew at 15‒37 °C (optimum, 25‒30 °C), pH 7‒11 (optimum, pH 7-8) and in the presence of 0‒8% (w/v) NaCl (optimum, 0 %).
32302275	14	22	theme	phenotypic	1511:1520	arg1	analyses					1569:1576	phenotypic, chemotaxonomic, phylogenetic and phylogenomic analyses	1511:1576	phenotypic, chemotaxonomic, phylogenetic and phylogenomic analyses	1511:1576	Based on the results of phenotypic, chemotaxonomic, phylogenetic and phylogenomic analyses, strain F435T represents a novel specie of the genus Auraticoccus, for which the name Auraticoccus cholistanensis sp.
32302275	5	23	theme	novel	503:507	arg1	strain					509:514	The novel strain	499:514	The novel strain	499:514	The novel strain grew at 15‒37 °C (optimum, 25‒30 °C), pH 7‒11 (optimum, pH 7-8) and in the presence of 0‒8% (w/v) NaCl (optimum, 0 %).
32302275	2	24	attach	isolated	256:263	arg1	sample					275:280	soil sample	270:280	soil sample collected from the Cholistan Desert, Pakistan	270:326	A Gram-stain-positive, aerobic, non-motile and non-spore-forming actinobacterium, designated as F435T, was isolated from soil sample collected from the Cholistan Desert, Pakistan.
32302275	2	24	attach	isolated	256:263	arg2	actinobacterium					214:228	A Gram-stain-positive, aerobic, non-motile and non-spore-forming actinobacterium	149:228	A Gram-stain-positive, aerobic, non-motile and non-spore-forming actinobacterium	149:228	A Gram-stain-positive, aerobic, non-motile and non-spore-forming actinobacterium, designated as F435T, was isolated from soil sample collected from the Cholistan Desert, Pakistan.
32302275	14	25	theme	cholistanensis	1677:1690	arg1	sp					1692:1693	the name Auraticoccus cholistanensis sp	1655:1693	the name Auraticoccus cholistanensis sp	1655:1693	Based on the results of phenotypic, chemotaxonomic, phylogenetic and phylogenomic analyses, strain F435T represents a novel specie of the genus Auraticoccus, for which the name Auraticoccus cholistanensis sp.
32302275	14	26	theme	name	1659:1662	arg1	sp					1692:1693	the name Auraticoccus cholistanensis sp	1655:1693	the name Auraticoccus cholistanensis sp	1655:1693	Based on the results of phenotypic, chemotaxonomic, phylogenetic and phylogenomic analyses, strain F435T represents a novel specie of the genus Auraticoccus, for which the name Auraticoccus cholistanensis sp.
32302275	8	27	from	present	982:988	arg1	wall					1002:1005	the cell wall	993:1005	the cell wall	993:1005	The peptidoglycan isomer present in the cell wall was ll-diaminopimelic acid.
32302275	10	28	theme	polar	1109:1113	arg1	lipids					1115:1120	Characteristic polar lipids	1094:1120	Characteristic polar lipids of the strain	1094:1134	Characteristic polar lipids of the strain were diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipids and glycolipids.
32302275	3	29	theme	taxonomic	333:341	arg1	position					343:350	The taxonomic position	329:350	The taxonomic position of the strain	329:364	The taxonomic position of the strain was established by using a polyphasic taxonomic approach.
32302275	4	30	theme	or	474:475	arg1	arrangement					477:487	single or arrangement	467:487	single or arrangement of pairs	467:496	The cells were coccoid-shaped and found in single or arrangement of pairs.
32302275	10	31	theme	strain	1129:1134	arg1	lipids					1115:1120	Characteristic polar lipids	1094:1120	Characteristic polar lipids of the strain	1094:1134	Characteristic polar lipids of the strain were diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipids and glycolipids.
32302275	13	32	theme	nucleotide	1371:1380	arg1	identity					1382:1389	average nucleotide identity	1363:1389	average nucleotide identity	1363:1389	The digital DNA-DNA hybridization (GGDC) and average nucleotide identity (ANI) values between strain F435T and A. monumenti MON 2.2T were 24.6 and 81.8 %, respectively.
32302275	0	33	theme	Auraticoccus	0:11	arg1	sp					28:29	Auraticoccus cholistanensis sp	0:29	Auraticoccus cholistanensis sp.	0:30	Auraticoccus cholistanensis sp.
32302275	1	34	theme	Cholistan	80:88	arg1	Desert					90:95	the Cholistan Desert	76:95	the Cholistan Desert	76:95	nov., an actinomycete isolated from soil of the Cholistan Desert, and emended description of the genus Auraticoccus.
32302275	3	35	theme	strain	359:364	arg1	position					343:350	The taxonomic position	329:350	The taxonomic position of the strain	329:364	The taxonomic position of the strain was established by using a polyphasic taxonomic approach.
32302275	13	36	theme	hybridization	1338:1350	arg1	%					1470:1470	24.6 and 81.8 %	1456:1470	24.6 and 81.8 %	1456:1470	The digital DNA-DNA hybridization (GGDC) and average nucleotide identity (ANI) values between strain F435T and A. monumenti MON 2.2T were 24.6 and 81.8 %, respectively.
32302275	13	36	theme	hybridization	1338:1350	arg1	values					1397:1402	The digital DNA-DNA hybridization (GGDC) and average nucleotide identity (ANI) values	1318:1402	The digital DNA-DNA hybridization (GGDC) and average nucleotide identity (ANI) values between strain F435T and A. monumenti MON 2.2T	1318:1449	The digital DNA-DNA hybridization (GGDC) and average nucleotide identity (ANI) values between strain F435T and A. monumenti MON 2.2T were 24.6 and 81.8 %, respectively.
32302275	6	37	theme	closest	746:752	arg1	relative					754:761	its closest relative	742:761	its closest relative with 97.4 % similarity followed by Desertihabitans aurantiacus CPCC 204711T (95.2 %)	742:846	Results of blast analysis based on 16S rRNA gene sequences showed that Auraticoccus monumenti MON 2.2T was its closest relative with 97.4 % similarity followed by Desertihabitans aurantiacus CPCC 204711T (95.2 %).
32302275	3	38	theme	polyphasic	393:402	arg1	approach					414:421	a polyphasic taxonomic approach	391:421	a polyphasic taxonomic approach	391:421	The taxonomic position of the strain was established by using a polyphasic taxonomic approach.
32302275	14	39	theme	Auraticoccus	1631:1642	arg1	specie					1611:1616	a novel specie	1603:1616	a novel specie	1603:1616	Based on the results of phenotypic, chemotaxonomic, phylogenetic and phylogenomic analyses, strain F435T represents a novel specie of the genus Auraticoccus, for which the name Auraticoccus cholistanensis sp.
32302275	16	40	theme	33648T=CGMCC	1745:1756	arg1	F435T					1733:1737	F435T	1733:1737	F435T (=JCM 33648T=CGMCC 1.17443T)	1733:1766	The type strain is F435T (=JCM 33648T=CGMCC 1.17443T).
32302275	16	40	theme	33648T=CGMCC	1745:1756	arg1	1.17443T					1758:1765	=JCM 33648T=CGMCC 1.17443T	1740:1765	=JCM 33648T=CGMCC 1.17443T	1740:1765	The type strain is F435T (=JCM 33648T=CGMCC 1.17443T).
32302275	14	41	theme	Auraticoccus	1664:1675	arg1	sp					1692:1693	the name Auraticoccus cholistanensis sp	1655:1693	the name Auraticoccus cholistanensis sp	1655:1693	Based on the results of phenotypic, chemotaxonomic, phylogenetic and phylogenomic analyses, strain F435T represents a novel specie of the genus Auraticoccus, for which the name Auraticoccus cholistanensis sp.
32302275	8	42	theme	peptidoglycan	961:973	arg1	acid					1029:1032	ll-diaminopimelic acid	1011:1032	ll-diaminopimelic acid	1011:1032	The peptidoglycan isomer present in the cell wall was ll-diaminopimelic acid.
32302275	8	42	theme	peptidoglycan	961:973	arg1	present					982:988	The peptidoglycan isomer present	957:988	The peptidoglycan isomer present in the cell wall	957:1005	The peptidoglycan isomer present in the cell wall was ll-diaminopimelic acid.
32302275	6	43	theme	MON	729:731	arg1	2.2T					733:736	Auraticoccus monumenti MON 2.2T	706:736	Auraticoccus monumenti MON 2.2T	706:736	Results of blast analysis based on 16S rRNA gene sequences showed that Auraticoccus monumenti MON 2.2T was its closest relative with 97.4 % similarity followed by Desertihabitans aurantiacus CPCC 204711T (95.2 %).
32302275	5	44	dep	optimum	563:569	arg1	pH					572:573	pH 7-8	572:577	pH 7-8	572:577	The novel strain grew at 15‒37 °C (optimum, 25‒30 °C), pH 7‒11 (optimum, pH 7-8) and in the presence of 0‒8% (w/v) NaCl (optimum, 0 %).
32302275	13	45	theme	digital	1322:1328	arg1	GGDC					1353:1356	GGDC	1353:1356	GGDC	1353:1356	The digital DNA-DNA hybridization (GGDC) and average nucleotide identity (ANI) values between strain F435T and A. monumenti MON 2.2T were 24.6 and 81.8 %, respectively.
32302275	13	45	theme	digital	1322:1328	arg1	hybridization					1338:1350	digital DNA-DNA hybridization	1322:1350	digital DNA-DNA hybridization (GGDC)	1322:1357	The digital DNA-DNA hybridization (GGDC) and average nucleotide identity (ANI) values between strain F435T and A. monumenti MON 2.2T were 24.6 and 81.8 %, respectively.
32302275	13	45	theme	digital	1322:1328	arg1	ANI					1392:1394	ANI	1392:1394	ANI	1392:1394	The digital DNA-DNA hybridization (GGDC) and average nucleotide identity (ANI) values between strain F435T and A. monumenti MON 2.2T were 24.6 and 81.8 %, respectively.
32302275	6	46	theme	Auraticoccus	706:717	arg1	2.2T					733:736	Auraticoccus monumenti MON 2.2T	706:736	Auraticoccus monumenti MON 2.2T	706:736	Results of blast analysis based on 16S rRNA gene sequences showed that Auraticoccus monumenti MON 2.2T was its closest relative with 97.4 % similarity followed by Desertihabitans aurantiacus CPCC 204711T (95.2 %).
32302275	1	47	theme	genus	129:133	arg1	Auraticoccus					135:146	the genus Auraticoccus	125:146	the genus Auraticoccus	125:146	nov., an actinomycete isolated from soil of the Cholistan Desert, and emended description of the genus Auraticoccus.
32302275	17	48	theme	Auraticoccus	1798:1809	arg1	description					1773:1783	The description	1769:1783	The description of the genus Auraticoccus	1769:1809	The description of the genus Auraticoccus has also been emended.
32302275	6	49	theme	Desertihabitans	798:812	arg1	204711T					831:837	Desertihabitans aurantiacus CPCC 204711T	798:837	Desertihabitans aurantiacus CPCC 204711T (95.2 %)	798:846	Results of blast analysis based on 16S rRNA gene sequences showed that Auraticoccus monumenti MON 2.2T was its closest relative with 97.4 % similarity followed by Desertihabitans aurantiacus CPCC 204711T (95.2 %).
32302275	6	49	theme	Desertihabitans	798:812	arg1	%					845:845	95.2 %	840:845	95.2 %	840:845	Results of blast analysis based on 16S rRNA gene sequences showed that Auraticoccus monumenti MON 2.2T was its closest relative with 97.4 % similarity followed by Desertihabitans aurantiacus CPCC 204711T (95.2 %).
32302275	6	50	with	relative	754:761	arg1	similarity					775:784	97.4 % similarity	768:784	97.4 % similarity followed by Desertihabitans aurantiacus CPCC 204711T (95.2 %)	768:846	Results of blast analysis based on 16S rRNA gene sequences showed that Auraticoccus monumenti MON 2.2T was its closest relative with 97.4 % similarity followed by Desertihabitans aurantiacus CPCC 204711T (95.2 %).
32302275	9	51	theme	fatty	1045:1049	arg1	acid					1051:1054	The major fatty acid	1035:1054	The major fatty acid	1035:1054	The major fatty acid was determined to be anteiso-C15 : 0.
32302275	9	51	theme	fatty	1045:1049	arg1	 0					1090:1091	 0	1090:1091	 0	1090:1091	The major fatty acid was determined to be anteiso-C15 : 0.
32302275	8	52	theme	ll-diaminopimelic	1011:1027	arg1	acid					1029:1032	ll-diaminopimelic acid	1011:1032	ll-diaminopimelic acid	1011:1032	The peptidoglycan isomer present in the cell wall was ll-diaminopimelic acid.
32302275	8	52	theme	ll-diaminopimelic	1011:1027	arg1	present					982:988	The peptidoglycan isomer present	957:988	The peptidoglycan isomer present in the cell wall	957:1005	The peptidoglycan isomer present in the cell wall was ll-diaminopimelic acid.
32302275	6	53	theme	%	773:773	arg1	similarity					775:784	97.4 % similarity	768:784	97.4 % similarity followed by Desertihabitans aurantiacus CPCC 204711T (95.2 %)	768:846	Results of blast analysis based on 16S rRNA gene sequences showed that Auraticoccus monumenti MON 2.2T was its closest relative with 97.4 % similarity followed by Desertihabitans aurantiacus CPCC 204711T (95.2 %).
32302275	12	54	theme	genomic	1269:1275	arg1	%					1315:1315	73.5 mol%	1307:1315	73.5 mol%	1307:1315	The genomic G+C content was calculated as 73.5 mol%.
32302275	12	54	theme	genomic	1269:1275	arg1	content					1281:1287	The genomic G+C content	1265:1287	The genomic G+C content	1265:1287	The genomic G+C content was calculated as 73.5 mol%.
32302275	6	55	theme	rRNA	674:677	arg1	sequences					684:692	16S rRNA gene sequences	670:692	16S rRNA gene sequences	670:692	Results of blast analysis based on 16S rRNA gene sequences showed that Auraticoccus monumenti MON 2.2T was its closest relative with 97.4 % similarity followed by Desertihabitans aurantiacus CPCC 204711T (95.2 %).
32302275	2	56	theme	soil	270:273	arg1	sample					275:280	soil sample	270:280	soil sample collected from the Cholistan Desert, Pakistan	270:326	A Gram-stain-positive, aerobic, non-motile and non-spore-forming actinobacterium, designated as F435T, was isolated from soil sample collected from the Cholistan Desert, Pakistan.
32302275	5	57	dep	15‒37 °C	524:531	arg1	25‒30 °C					543:550	25‒30 °C	543:550	25‒30 °C	543:550	The novel strain grew at 15‒37 °C (optimum, 25‒30 °C), pH 7‒11 (optimum, pH 7-8) and in the presence of 0‒8% (w/v) NaCl (optimum, 0 %).
32302275	5	57	dep	15‒37 °C	524:531	arg1	optimum					534:540	optimum	534:540	optimum	534:540	The novel strain grew at 15‒37 °C (optimum, 25‒30 °C), pH 7‒11 (optimum, pH 7-8) and in the presence of 0‒8% (w/v) NaCl (optimum, 0 %).
32302275	7	58	theme	genus	937:941	arg1	Auraticoccus					943:954	the genus Auraticoccus	933:954	the genus Auraticoccus	933:954	In phylogenetic trees, strain F435T formed a robust cluster with the only member of the genus Auraticoccus.
32302275	13	59	theme	A.	1429:1430	arg1	2.2T					1446:1449	A. monumenti MON 2.2T	1429:1449	A. monumenti MON 2.2T	1429:1449	The digital DNA-DNA hybridization (GGDC) and average nucleotide identity (ANI) values between strain F435T and A. monumenti MON 2.2T were 24.6 and 81.8 %, respectively.
32302275	1	60	attach	isolated	54:61	arg2	actinomycete					41:52	an actinomycete	38:52	an actinomycete isolated from soil of the Cholistan Desert	38:95	nov., an actinomycete isolated from soil of the Cholistan Desert, and emended description of the genus Auraticoccus.
32302275	1	60	attach	isolated	54:61	arg1	soil					68:71	soil	68:71	soil of the Cholistan Desert	68:95	nov., an actinomycete isolated from soil of the Cholistan Desert, and emended description of the genus Auraticoccus.
32302275	6	61	theme	analysis	652:659	arg1	Results					635:641	Results	635:641	Results of blast analysis based on 16S rRNA gene sequences	635:692	Results of blast analysis based on 16S rRNA gene sequences showed that Auraticoccus monumenti MON 2.2T was its closest relative with 97.4 % similarity followed by Desertihabitans aurantiacus CPCC 204711T (95.2 %).
32302275	13	62	theme	MON	1442:1444	arg1	2.2T					1446:1449	A. monumenti MON 2.2T	1429:1449	A. monumenti MON 2.2T	1429:1449	The digital DNA-DNA hybridization (GGDC) and average nucleotide identity (ANI) values between strain F435T and A. monumenti MON 2.2T were 24.6 and 81.8 %, respectively.
32302275	7	63	theme	only	918:921	arg1	member					923:928	the only member	914:928	the only member of the genus Auraticoccus	914:954	In phylogenetic trees, strain F435T formed a robust cluster with the only member of the genus Auraticoccus.
32302275	16	64	theme	=JCM	1740:1743	arg1	F435T					1733:1737	F435T	1733:1737	F435T (=JCM 33648T=CGMCC 1.17443T)	1733:1766	The type strain is F435T (=JCM 33648T=CGMCC 1.17443T).
32302275	16	64	theme	=JCM	1740:1743	arg1	1.17443T					1758:1765	=JCM 33648T=CGMCC 1.17443T	1740:1765	=JCM 33648T=CGMCC 1.17443T	1740:1765	The type strain is F435T (=JCM 33648T=CGMCC 1.17443T).
32302275	6	65	theme	CPCC	826:829	arg1	204711T					831:837	Desertihabitans aurantiacus CPCC 204711T	798:837	Desertihabitans aurantiacus CPCC 204711T (95.2 %)	798:846	Results of blast analysis based on 16S rRNA gene sequences showed that Auraticoccus monumenti MON 2.2T was its closest relative with 97.4 % similarity followed by Desertihabitans aurantiacus CPCC 204711T (95.2 %).
32302275	6	65	theme	CPCC	826:829	arg1	%					845:845	95.2 %	840:845	95.2 %	840:845	Results of blast analysis based on 16S rRNA gene sequences showed that Auraticoccus monumenti MON 2.2T was its closest relative with 97.4 % similarity followed by Desertihabitans aurantiacus CPCC 204711T (95.2 %).
32302275	10	66	theme	Characteristic	1094:1107	arg1	lipids					1115:1120	Characteristic polar lipids	1094:1120	Characteristic polar lipids of the strain	1094:1134	Characteristic polar lipids of the strain were diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipids and glycolipids.
32302275	7	67	theme	robust	894:899	arg1	cluster					901:907	a robust cluster	892:907	a robust cluster	892:907	In phylogenetic trees, strain F435T formed a robust cluster with the only member of the genus Auraticoccus.
32302275	5	68	dep	optimum	620:626	arg1	%					631:631	0 %	629:631	0 %	629:631	The novel strain grew at 15‒37 °C (optimum, 25‒30 °C), pH 7‒11 (optimum, pH 7-8) and in the presence of 0‒8% (w/v) NaCl (optimum, 0 %).
32302275	4	69	theme	pairs	492:496	arg1	arrangement					477:487	single or arrangement	467:487	single or arrangement of pairs	467:496	The cells were coccoid-shaped and found in single or arrangement of pairs.
32302275	7	70	theme	phylogenetic	852:863	arg1	trees					865:869	phylogenetic trees	852:869	phylogenetic trees	852:869	In phylogenetic trees, strain F435T formed a robust cluster with the only member of the genus Auraticoccus.
32302275	1	71	theme	Auraticoccus	135:146	arg1	description					110:120	emended description	102:120	emended description of the genus Auraticoccus	102:146	nov., an actinomycete isolated from soil of the Cholistan Desert, and emended description of the genus Auraticoccus.
32302275	1	71	theme	Auraticoccus	135:146	arg1	nov.					32:35	nov.	32:35	nov.	32:35	nov., an actinomycete isolated from soil of the Cholistan Desert, and emended description of the genus Auraticoccus.
32302275	1	71	theme	Auraticoccus	135:146	arg1	actinomycete					41:52	an actinomycete	38:52	an actinomycete isolated from soil of the Cholistan Desert	38:95	nov., an actinomycete isolated from soil of the Cholistan Desert, and emended description of the genus Auraticoccus.
32302275	4	72	theme	single	467:472	arg1	arrangement					477:487	single or arrangement	467:487	single or arrangement of pairs	467:496	The cells were coccoid-shaped and found in single or arrangement of pairs.
32302275	13	73	theme	average	1363:1369	arg1	identity					1382:1389	average nucleotide identity	1363:1389	average nucleotide identity	1363:1389	The digital DNA-DNA hybridization (GGDC) and average nucleotide identity (ANI) values between strain F435T and A. monumenti MON 2.2T were 24.6 and 81.8 %, respectively.
32302275	0	74	theme	cholistanensis	13:26	arg1	sp					28:29	Auraticoccus cholistanensis sp	0:29	Auraticoccus cholistanensis sp.	0:30	Auraticoccus cholistanensis sp.
32302275	17	75	theme	genus	1792:1796	arg1	Auraticoccus					1798:1809	the genus Auraticoccus	1788:1809	the genus Auraticoccus	1788:1809	The description of the genus Auraticoccus has also been emended.
32302275	6	76	dep	Auraticoccus	706:717	arg1	monumenti					719:727	monumenti	719:727	monumenti	719:727	Results of blast analysis based on 16S rRNA gene sequences showed that Auraticoccus monumenti MON 2.2T was its closest relative with 97.4 % similarity followed by Desertihabitans aurantiacus CPCC 204711T (95.2 %).
32302275	13	77	theme	identity	1382:1389	arg1	%					1470:1470	24.6 and 81.8 %	1456:1470	24.6 and 81.8 %	1456:1470	The digital DNA-DNA hybridization (GGDC) and average nucleotide identity (ANI) values between strain F435T and A. monumenti MON 2.2T were 24.6 and 81.8 %, respectively.
32302275	13	77	theme	identity	1382:1389	arg1	values					1397:1402	The digital DNA-DNA hybridization (GGDC) and average nucleotide identity (ANI) values	1318:1402	The digital DNA-DNA hybridization (GGDC) and average nucleotide identity (ANI) values between strain F435T and A. monumenti MON 2.2T	1318:1449	The digital DNA-DNA hybridization (GGDC) and average nucleotide identity (ANI) values between strain F435T and A. monumenti MON 2.2T were 24.6 and 81.8 %, respectively.
32302275	1	78	theme	Desert	90:95	arg1	soil					68:71	soil	68:71	soil of the Cholistan Desert	68:95	nov., an actinomycete isolated from soil of the Cholistan Desert, and emended description of the genus Auraticoccus.
32302275	2	79	theme	aerobic	172:178	arg1	actinobacterium					214:228	A Gram-stain-positive, aerobic, non-motile and non-spore-forming actinobacterium	149:228	A Gram-stain-positive, aerobic, non-motile and non-spore-forming actinobacterium	149:228	A Gram-stain-positive, aerobic, non-motile and non-spore-forming actinobacterium, designated as F435T, was isolated from soil sample collected from the Cholistan Desert, Pakistan.
32302275	3	80	theme	taxonomic	404:412	arg1	approach					414:421	a polyphasic taxonomic approach	391:421	a polyphasic taxonomic approach	391:421	The taxonomic position of the strain was established by using a polyphasic taxonomic approach.
32302275	2	81	theme	Gram-stain-positive	151:169	arg1	actinobacterium					214:228	A Gram-stain-positive, aerobic, non-motile and non-spore-forming actinobacterium	149:228	A Gram-stain-positive, aerobic, non-motile and non-spore-forming actinobacterium	149:228	A Gram-stain-positive, aerobic, non-motile and non-spore-forming actinobacterium, designated as F435T, was isolated from soil sample collected from the Cholistan Desert, Pakistan.
32302275	6	82	theme	blast	646:650	arg1	analysis					652:659	blast analysis	646:659	blast analysis based on 16S rRNA gene sequences	646:692	Results of blast analysis based on 16S rRNA gene sequences showed that Auraticoccus monumenti MON 2.2T was its closest relative with 97.4 % similarity followed by Desertihabitans aurantiacus CPCC 204711T (95.2 %).
32302275	14	83	theme	genus	1625:1629	arg1	Auraticoccus					1631:1642	the genus Auraticoccus	1621:1642	the genus Auraticoccus	1621:1642	Based on the results of phenotypic, chemotaxonomic, phylogenetic and phylogenomic analyses, strain F435T represents a novel specie of the genus Auraticoccus, for which the name Auraticoccus cholistanensis sp.
32302275	8	84	theme	isomer	975:980	arg1	acid					1029:1032	ll-diaminopimelic acid	1011:1032	ll-diaminopimelic acid	1011:1032	The peptidoglycan isomer present in the cell wall was ll-diaminopimelic acid.
32302275	8	84	theme	isomer	975:980	arg1	present					982:988	The peptidoglycan isomer present	957:988	The peptidoglycan isomer present in the cell wall	957:1005	The peptidoglycan isomer present in the cell wall was ll-diaminopimelic acid.
32302275	2	85	theme	non-spore-forming	196:212	arg1	actinobacterium					214:228	A Gram-stain-positive, aerobic, non-motile and non-spore-forming actinobacterium	149:228	A Gram-stain-positive, aerobic, non-motile and non-spore-forming actinobacterium	149:228	A Gram-stain-positive, aerobic, non-motile and non-spore-forming actinobacterium, designated as F435T, was isolated from soil sample collected from the Cholistan Desert, Pakistan.
32302275	2	86	theme	non-motile	181:190	arg1	actinobacterium					214:228	A Gram-stain-positive, aerobic, non-motile and non-spore-forming actinobacterium	149:228	A Gram-stain-positive, aerobic, non-motile and non-spore-forming actinobacterium	149:228	A Gram-stain-positive, aerobic, non-motile and non-spore-forming actinobacterium, designated as F435T, was isolated from soil sample collected from the Cholistan Desert, Pakistan.
32302275	13	87	theme	DNA-DNA	1330:1336	arg1	GGDC					1353:1356	GGDC	1353:1356	GGDC	1353:1356	The digital DNA-DNA hybridization (GGDC) and average nucleotide identity (ANI) values between strain F435T and A. monumenti MON 2.2T were 24.6 and 81.8 %, respectively.
32302275	13	87	theme	DNA-DNA	1330:1336	arg1	hybridization					1338:1350	digital DNA-DNA hybridization	1322:1350	digital DNA-DNA hybridization (GGDC)	1322:1357	The digital DNA-DNA hybridization (GGDC) and average nucleotide identity (ANI) values between strain F435T and A. monumenti MON 2.2T were 24.6 and 81.8 %, respectively.
32302275	13	87	theme	DNA-DNA	1330:1336	arg1	ANI					1392:1394	ANI	1392:1394	ANI	1392:1394	The digital DNA-DNA hybridization (GGDC) and average nucleotide identity (ANI) values between strain F435T and A. monumenti MON 2.2T were 24.6 and 81.8 %, respectively.
32302275	14	88	theme	novel	1605:1609	arg1	specie					1611:1616	a novel specie	1603:1616	a novel specie	1603:1616	Based on the results of phenotypic, chemotaxonomic, phylogenetic and phylogenomic analyses, strain F435T represents a novel specie of the genus Auraticoccus, for which the name Auraticoccus cholistanensis sp.
32302275	8	89	theme	cell	997:1000	arg1	wall					1002:1005	the cell wall	993:1005	the cell wall	993:1005	The peptidoglycan isomer present in the cell wall was ll-diaminopimelic acid.
34421901	1	0	theme	detrimental	258:268	arg1	effects					290:296	detrimental immune/inflammatory effects	258:296	detrimental immune/inflammatory effects	258:296	Engineered nanoparticles used for medical purposes must meet stringent safety criteria, which include immunosafety, i.e., the inability to activate possibly detrimental immune/inflammatory effects.
34421901	8	1	dep	diameter	1633:1640	arg1	particles					1663:1671	spherical iron oxide particles	1642:1671	17 vs. 22 nm diameter spherical iron oxide particles	1620:1671	By comparing nanomaterials of different shapes (spherical vs. rod-shaped gold particles) and different size (17 vs. 22 nm diameter spherical iron oxide particles), it was evident that innate memory could be differentially induced and modulated depending on size, shape and chemical composition.
34421901	8	1	dep	diameter	1633:1640	arg1	nm					1630:1631	nm	1630:1631	nm	1630:1631	By comparing nanomaterials of different shapes (spherical vs. rod-shaped gold particles) and different size (17 vs. 22 nm diameter spherical iron oxide particles), it was evident that innate memory could be differentially induced and modulated depending on size, shape and chemical composition.
34421901	8	2	theme	different	1541:1549	arg1	shapes					1551:1556	different shapes	1541:1556	different shapes	1541:1556	By comparing nanomaterials of different shapes (spherical vs. rod-shaped gold particles) and different size (17 vs. 22 nm diameter spherical iron oxide particles), it was evident that innate memory could be differentially induced and modulated depending on size, shape and chemical composition.
34421901	9	3	theme	same	2000:2003	arg1	particles					2005:2013	the same particles	1996:2013	the same particles	1996:2013	However, the main finding was that the innate memory effect of the particles was strongly donor-dependent, with monocytes from each donor showing a distinct memory profile upon priming with the same particles, thereby making impossible to draw general conclusions on the particle effects.
34421901	2	4	theme	medical	304:310	arg1	nanomaterials					312:324	Even medical nanomaterials	299:324	Even medical nanomaterials devoid of direct immunotoxic or inflammatory effects	299:377	Even medical nanomaterials devoid of direct immunotoxic or inflammatory effects may have an impact on human health if able to modify innate memory, which is the ability to "prime" future immune responses towards a different, possibly more detrimental reactivity.
34421901	8	5	theme	different	1604:1612	arg1	size					1614:1617	different size	1604:1617	different size (17 vs. 22 nm diameter spherical iron oxide particles)	1604:1672	By comparing nanomaterials of different shapes (spherical vs. rod-shaped gold particles) and different size (17 vs. 22 nm diameter spherical iron oxide particles), it was evident that innate memory could be differentially induced and modulated depending on size, shape and chemical composition.
34421901	6	6	theme	LPS	1195:1197	arg1	challenge					1199:1207	the LPS challenge	1191:1207	the LPS challenge	1191:1207	The memory response to the LPS challenge was measured as changes in the production of inflammatory (TNFα, IL-6) and anti-inflammatory cytokines (IL-10, IL-1Ra), as compared to unprimed monocytes.
34421901	0	7	theme	Personalised	0:11	arg1	Profiling					13:21	Personalised Profiling	0:21	Personalised Profiling of Innate Immune Memory	0:45	Personalised Profiling of Innate Immune Memory Induced by Nano-Imaging Particles in Human Monocytes.
34421901	10	8	theme	nanoparticles	2143:2155	arg1	effect					2125:2130	the effect	2121:2130	the effect of imaging nanoparticles on the innate memory of patients	2121:2188	Thus, in order to predict the effect of imaging nanoparticles on the innate memory of patients, a personalised profiling would be required, able to take in consideration the peculiarities of the individual innate immune reactivity.
34421901	2	9	theme	direct	336:341	arg1	effects					371:377	direct immunotoxic or inflammatory effects	336:377	direct immunotoxic or inflammatory effects	336:377	Even medical nanomaterials devoid of direct immunotoxic or inflammatory effects may have an impact on human health if able to modify innate memory, which is the ability to "prime" future immune responses towards a different, possibly more detrimental reactivity.
34421901	7	10	contain	have	1418:1421	arg1	types					1391:1395	both types	1386:1395	both types of nanoparticles	1386:1412	The results show that both types of nanoparticles can have an effect in the induction of memory, with changes observed in the cytokine production.
34421901	7	10	contain	have	1418:1421	arg2	effect					1426:1431	an effect	1423:1431	an effect	1423:1431	The results show that both types of nanoparticles can have an effect in the induction of memory, with changes observed in the cytokine production.
34421901	10	11	theme	patients	2181:2188	arg1	memory					2171:2176	the innate memory	2160:2176	the innate memory of patients	2160:2188	Thus, in order to predict the effect of imaging nanoparticles on the innate memory of patients, a personalised profiling would be required, able to take in consideration the peculiarities of the individual innate immune reactivity.
34421901	2	12	from	impact	391:396	arg1	health					407:412	human health	401:412	human health	401:412	Even medical nanomaterials devoid of direct immunotoxic or inflammatory effects may have an impact on human health if able to modify innate memory, which is the ability to "prime" future immune responses towards a different, possibly more detrimental reactivity.
34421901	9	13	with	donor-dependent	1896:1910	arg1	monocytes					1918:1926	monocytes	1918:1926	monocytes from each donor showing a distinct memory profile upon priming with the same particles	1918:2013	However, the main finding was that the innate memory effect of the particles was strongly donor-dependent, with monocytes from each donor showing a distinct memory profile upon priming with the same particles, thereby making impossible to draw general conclusions on the particle effects.
34421901	6	14	theme	TNFα	1268:1271	arg1	production					1240:1249	the production	1236:1249	the production of inflammatory (TNFα, IL-6) and anti-inflammatory cytokines (IL-10, IL-1Ra)	1236:1326	The memory response to the LPS challenge was measured as changes in the production of inflammatory (TNFα, IL-6) and anti-inflammatory cytokines (IL-10, IL-1Ra), as compared to unprimed monocytes.
34421901	5	15	theme	priming	1026:1032	arg1	LPS					1021:1023	the bacterial agent LPS	1001:1023	the bacterial agent LPS (priming phase/primary response)	1001:1056	Monocytes were exposed in culture to nanoparticles alone or together with the bacterial agent LPS (priming phase/primary response), then rested for six days (extinction phase), and eventually challenged with LPS (memory/secondary response).
34421901	5	15	theme	priming	1026:1032	arg1	response					1048:1055	priming phase/primary response	1026:1055	priming phase/primary response	1026:1055	Monocytes were exposed in culture to nanoparticles alone or together with the bacterial agent LPS (priming phase/primary response), then rested for six days (extinction phase), and eventually challenged with LPS (memory/secondary response).
34421901	2	16	theme	devoid	326:331	arg1	nanomaterials					312:324	Even medical nanomaterials	299:324	Even medical nanomaterials devoid of direct immunotoxic or inflammatory effects	299:377	Even medical nanomaterials devoid of direct immunotoxic or inflammatory effects may have an impact on human health if able to modify innate memory, which is the ability to "prime" future immune responses towards a different, possibly more detrimental reactivity.
34421901	9	17	theme	main	1819:1822	arg1	finding					1824:1830	the main finding	1815:1830	the main finding	1815:1830	However, the main finding was that the innate memory effect of the particles was strongly donor-dependent, with monocytes from each donor showing a distinct memory profile upon priming with the same particles, thereby making impossible to draw general conclusions on the particle effects.
34421901	9	18	theme	general	2050:2056	arg1	conclusions					2058:2068	general conclusions	2050:2068	general conclusions	2050:2068	However, the main finding was that the innate memory effect of the particles was strongly donor-dependent, with monocytes from each donor showing a distinct memory profile upon priming with the same particles, thereby making impossible to draw general conclusions on the particle effects.
34421901	5	19	theme	memory/secondary	1140:1155	arg1	response					1157:1164	memory/secondary response	1140:1164	memory/secondary response	1140:1164	Monocytes were exposed in culture to nanoparticles alone or together with the bacterial agent LPS (priming phase/primary response), then rested for six days (extinction phase), and eventually challenged with LPS (memory/secondary response).
34421901	5	19	theme	memory/secondary	1140:1155	arg1	LPS					1135:1137	LPS	1135:1137	LPS (memory/secondary response)	1135:1165	Monocytes were exposed in culture to nanoparticles alone or together with the bacterial agent LPS (priming phase/primary response), then rested for six days (extinction phase), and eventually challenged with LPS (memory/secondary response).
34421901	8	20	theme	spherical	1642:1650	arg1	particles					1663:1671	spherical iron oxide particles	1642:1671	17 vs. 22 nm diameter spherical iron oxide particles	1620:1671	By comparing nanomaterials of different shapes (spherical vs. rod-shaped gold particles) and different size (17 vs. 22 nm diameter spherical iron oxide particles), it was evident that innate memory could be differentially induced and modulated depending on size, shape and chemical composition.
34421901	1	21	dep	inability	227:235	arg1	i.e.					217:220	i.e.	217:220	i.e.	217:220	Engineered nanoparticles used for medical purposes must meet stringent safety criteria, which include immunosafety, i.e., the inability to activate possibly detrimental immune/inflammatory effects.
34421901	5	22	theme	bacterial	1005:1013	arg1	LPS					1021:1023	the bacterial agent LPS	1001:1023	the bacterial agent LPS (priming phase/primary response)	1001:1056	Monocytes were exposed in culture to nanoparticles alone or together with the bacterial agent LPS (priming phase/primary response), then rested for six days (extinction phase), and eventually challenged with LPS (memory/secondary response).
34421901	5	22	theme	bacterial	1005:1013	arg1	response					1048:1055	priming phase/primary response	1026:1055	priming phase/primary response	1026:1055	Monocytes were exposed in culture to nanoparticles alone or together with the bacterial agent LPS (priming phase/primary response), then rested for six days (extinction phase), and eventually challenged with LPS (memory/secondary response).
34421901	8	23	theme	oxide	1657:1661	arg1	particles					1663:1671	spherical iron oxide particles	1642:1671	17 vs. 22 nm diameter spherical iron oxide particles	1620:1671	By comparing nanomaterials of different shapes (spherical vs. rod-shaped gold particles) and different size (17 vs. 22 nm diameter spherical iron oxide particles), it was evident that innate memory could be differentially induced and modulated depending on size, shape and chemical composition.
34421901	4	24	theme	in	878:879	arg1	model					887:891	an in vitro model	875:891	an in vitro model based on human primary monocytes	875:924	In this study, we have examined the ability of two nanomaterials commonly used for diagnostic imaging purposes, gold and iron oxide nanoparticles, to induce or modulate innate memory, using an in vitro model based on human primary monocytes.
34421901	9	25	theme	particle	2077:2084	arg1	effects					2086:2092	the particle effects	2073:2092	the particle effects	2073:2092	However, the main finding was that the innate memory effect of the particles was strongly donor-dependent, with monocytes from each donor showing a distinct memory profile upon priming with the same particles, thereby making impossible to draw general conclusions on the particle effects.
34421901	9	26	theme	memory	1852:1857	arg1	effect					1859:1864	the innate memory effect	1841:1864	the innate memory effect of the particles	1841:1881	However, the main finding was that the innate memory effect of the particles was strongly donor-dependent, with monocytes from each donor showing a distinct memory profile upon priming with the same particles, thereby making impossible to draw general conclusions on the particle effects.
34421901	9	26	theme	memory	1852:1857	arg1	donor-dependent					1896:1910	donor-dependent	1896:1910	donor-dependent	1896:1910	However, the main finding was that the innate memory effect of the particles was strongly donor-dependent, with monocytes from each donor showing a distinct memory profile upon priming with the same particles, thereby making impossible to draw general conclusions on the particle effects.
34421901	0	27	theme	Nano-Imaging	58:69	arg1	Particles					71:79	Nano-Imaging Particles	58:79	Nano-Imaging Particles in Human Monocytes	58:98	Personalised Profiling of Innate Immune Memory Induced by Nano-Imaging Particles in Human Monocytes.
34421901	3	28	theme	innate	618:623	arg1	responses					632:640	anomalous innate memory responses	608:640	anomalous innate memory responses	608:640	Although innate memory is usually protective, anomalous innate memory responses may be at the basis of immune pathologies.
34421901	2	29	theme	future	479:484	arg1	responses					493:501	future immune responses	479:501	future immune responses	479:501	Even medical nanomaterials devoid of direct immunotoxic or inflammatory effects may have an impact on human health if able to modify innate memory, which is the ability to "prime" future immune responses towards a different, possibly more detrimental reactivity.
34421901	6	30	theme	cytokines	1302:1310	arg1	production					1240:1249	the production	1236:1249	the production of inflammatory (TNFα, IL-6) and anti-inflammatory cytokines (IL-10, IL-1Ra)	1236:1326	The memory response to the LPS challenge was measured as changes in the production of inflammatory (TNFα, IL-6) and anti-inflammatory cytokines (IL-10, IL-1Ra), as compared to unprimed monocytes.
34421901	1	31	theme	safety	172:177	arg1	criteria					179:186	stringent safety criteria	162:186	stringent safety criteria	162:186	Engineered nanoparticles used for medical purposes must meet stringent safety criteria, which include immunosafety, i.e., the inability to activate possibly detrimental immune/inflammatory effects.
34421901	4	32	theme	primary	908:914	arg1	monocytes					916:924	human primary monocytes	902:924	human primary monocytes	902:924	In this study, we have examined the ability of two nanomaterials commonly used for diagnostic imaging purposes, gold and iron oxide nanoparticles, to induce or modulate innate memory, using an in vitro model based on human primary monocytes.
34421901	9	33	theme	particles	1873:1881	arg1	effect					1859:1864	the innate memory effect	1841:1864	the innate memory effect of the particles	1841:1881	However, the main finding was that the innate memory effect of the particles was strongly donor-dependent, with monocytes from each donor showing a distinct memory profile upon priming with the same particles, thereby making impossible to draw general conclusions on the particle effects.
34421901	9	33	theme	particles	1873:1881	arg1	donor-dependent					1896:1910	donor-dependent	1896:1910	donor-dependent	1896:1910	However, the main finding was that the innate memory effect of the particles was strongly donor-dependent, with monocytes from each donor showing a distinct memory profile upon priming with the same particles, thereby making impossible to draw general conclusions on the particle effects.
34421901	2	34	contain	have	383:386	arg1	nanomaterials					312:324	Even medical nanomaterials	299:324	Even medical nanomaterials devoid of direct immunotoxic or inflammatory effects	299:377	Even medical nanomaterials devoid of direct immunotoxic or inflammatory effects may have an impact on human health if able to modify innate memory, which is the ability to "prime" future immune responses towards a different, possibly more detrimental reactivity.
34421901	2	34	contain	have	383:386	arg2	impact					391:396	an impact	388:396	an impact on human health	388:412	Even medical nanomaterials devoid of direct immunotoxic or inflammatory effects may have an impact on human health if able to modify innate memory, which is the ability to "prime" future immune responses towards a different, possibly more detrimental reactivity.
34421901	8	35	theme	innate	1695:1700	arg1	memory					1702:1707	innate memory	1695:1707	innate memory	1695:1707	By comparing nanomaterials of different shapes (spherical vs. rod-shaped gold particles) and different size (17 vs. 22 nm diameter spherical iron oxide particles), it was evident that innate memory could be differentially induced and modulated depending on size, shape and chemical composition.
34421901	7	36	theme	memory	1453:1458	arg1	induction					1440:1448	the induction	1436:1448	the induction of memory	1436:1458	The results show that both types of nanoparticles can have an effect in the induction of memory, with changes observed in the cytokine production.
34421901	8	37	dep	size	1614:1617	arg1	diameter					1633:1640	17 vs. 22 nm diameter	1620:1640	17 vs. 22 nm diameter spherical iron oxide particles	1620:1671	By comparing nanomaterials of different shapes (spherical vs. rod-shaped gold particles) and different size (17 vs. 22 nm diameter spherical iron oxide particles), it was evident that innate memory could be differentially induced and modulated depending on size, shape and chemical composition.
34421901	6	38	theme	unprimed	1344:1351	arg1	monocytes					1353:1361	unprimed monocytes	1344:1361	unprimed monocytes	1344:1361	The memory response to the LPS challenge was measured as changes in the production of inflammatory (TNFα, IL-6) and anti-inflammatory cytokines (IL-10, IL-1Ra), as compared to unprimed monocytes.
34421901	6	39	dep	IL-10	1313:1317	arg1	IL-1Ra					1320:1325	IL-1Ra	1320:1325	IL-1Ra	1320:1325	The memory response to the LPS challenge was measured as changes in the production of inflammatory (TNFα, IL-6) and anti-inflammatory cytokines (IL-10, IL-1Ra), as compared to unprimed monocytes.
34421901	10	40	theme	immune	2308:2313	arg1	reactivity					2315:2324	the individual innate immune reactivity	2286:2324	the individual innate immune reactivity	2286:2324	Thus, in order to predict the effect of imaging nanoparticles on the innate memory of patients, a personalised profiling would be required, able to take in consideration the peculiarities of the individual innate immune reactivity.
34421901	10	41	from	effect	2125:2130	arg1	memory					2171:2176	the innate memory	2160:2176	the innate memory of patients	2160:2188	Thus, in order to predict the effect of imaging nanoparticles on the innate memory of patients, a personalised profiling would be required, able to take in consideration the peculiarities of the individual innate immune reactivity.
34421901	0	42	theme	Immune	33:38	arg1	Memory					40:45	Innate Immune Memory	26:45	Innate Immune Memory	26:45	Personalised Profiling of Innate Immune Memory Induced by Nano-Imaging Particles in Human Monocytes.
34421901	2	43	dep	different	513:521	arg1	detrimental					538:548	detrimental	538:548	detrimental	538:548	Even medical nanomaterials devoid of direct immunotoxic or inflammatory effects may have an impact on human health if able to modify innate memory, which is the ability to "prime" future immune responses towards a different, possibly more detrimental reactivity.
34421901	10	44	theme	individual	2290:2299	arg1	reactivity					2315:2324	the individual innate immune reactivity	2286:2324	the individual innate immune reactivity	2286:2324	Thus, in order to predict the effect of imaging nanoparticles on the innate memory of patients, a personalised profiling would be required, able to take in consideration the peculiarities of the individual innate immune reactivity.
34421901	4	45	theme	oxide	811:815	arg1	nanoparticles					817:829	iron oxide nanoparticles	806:829	iron oxide nanoparticles	806:829	In this study, we have examined the ability of two nanomaterials commonly used for diagnostic imaging purposes, gold and iron oxide nanoparticles, to induce or modulate innate memory, using an in vitro model based on human primary monocytes.
34421901	4	45	theme	oxide	811:815	arg1	nanomaterials					736:748	two nanomaterials	732:748	two nanomaterials commonly used for diagnostic imaging purposes	732:794	In this study, we have examined the ability of two nanomaterials commonly used for diagnostic imaging purposes, gold and iron oxide nanoparticles, to induce or modulate innate memory, using an in vitro model based on human primary monocytes.
34421901	2	46	dep	"	477:477	arg1	prime					472:476	prime	472:476	prime	472:476	Even medical nanomaterials devoid of direct immunotoxic or inflammatory effects may have an impact on human health if able to modify innate memory, which is the ability to "prime" future immune responses towards a different, possibly more detrimental reactivity.
34421901	3	47	theme	pathologies	672:682	arg1	basis					656:660	the basis	652:660	the basis of immune pathologies	652:682	Although innate memory is usually protective, anomalous innate memory responses may be at the basis of immune pathologies.
34421901	4	48	theme	imaging	779:785	arg1	purposes					787:794	diagnostic imaging purposes	768:794	diagnostic imaging purposes	768:794	In this study, we have examined the ability of two nanomaterials commonly used for diagnostic imaging purposes, gold and iron oxide nanoparticles, to induce or modulate innate memory, using an in vitro model based on human primary monocytes.
34421901	2	49	theme	inflammatory	358:369	arg1	effects					371:377	direct immunotoxic or inflammatory effects	336:377	direct immunotoxic or inflammatory effects	336:377	Even medical nanomaterials devoid of direct immunotoxic or inflammatory effects may have an impact on human health if able to modify innate memory, which is the ability to "prime" future immune responses towards a different, possibly more detrimental reactivity.
34421901	8	50	theme	shapes	1551:1556	arg1	size					1614:1617	different size	1604:1617	different size (17 vs. 22 nm diameter spherical iron oxide particles)	1604:1672	By comparing nanomaterials of different shapes (spherical vs. rod-shaped gold particles) and different size (17 vs. 22 nm diameter spherical iron oxide particles), it was evident that innate memory could be differentially induced and modulated depending on size, shape and chemical composition.
34421901	8	50	theme	shapes	1551:1556	arg1	nanomaterials					1524:1536	nanomaterials	1524:1536	nanomaterials of different shapes (spherical vs. rod-shaped gold particles)	1524:1598	By comparing nanomaterials of different shapes (spherical vs. rod-shaped gold particles) and different size (17 vs. 22 nm diameter spherical iron oxide particles), it was evident that innate memory could be differentially induced and modulated depending on size, shape and chemical composition.
34421901	8	50	theme	shapes	1551:1556	arg1	particles					1589:1597	spherical vs. rod-shaped gold particles	1559:1597	spherical vs. rod-shaped gold particles	1559:1597	By comparing nanomaterials of different shapes (spherical vs. rod-shaped gold particles) and different size (17 vs. 22 nm diameter spherical iron oxide particles), it was evident that innate memory could be differentially induced and modulated depending on size, shape and chemical composition.
34421901	7	51	theme	nanoparticles	1400:1412	arg1	types					1391:1395	both types	1386:1395	both types of nanoparticles	1386:1412	The results show that both types of nanoparticles can have an effect in the induction of memory, with changes observed in the cytokine production.
34421901	8	52	theme	spherical	1559:1567	arg1	nanomaterials					1524:1536	nanomaterials	1524:1536	nanomaterials of different shapes (spherical vs. rod-shaped gold particles)	1524:1598	By comparing nanomaterials of different shapes (spherical vs. rod-shaped gold particles) and different size (17 vs. 22 nm diameter spherical iron oxide particles), it was evident that innate memory could be differentially induced and modulated depending on size, shape and chemical composition.
34421901	8	52	theme	spherical	1559:1567	arg1	particles					1589:1597	spherical vs. rod-shaped gold particles	1559:1597	spherical vs. rod-shaped gold particles	1559:1597	By comparing nanomaterials of different shapes (spherical vs. rod-shaped gold particles) and different size (17 vs. 22 nm diameter spherical iron oxide particles), it was evident that innate memory could be differentially induced and modulated depending on size, shape and chemical composition.
34421901	6	53	from	changes	1225:1231	arg1	production					1240:1249	the production	1236:1249	the production of inflammatory (TNFα, IL-6) and anti-inflammatory cytokines (IL-10, IL-1Ra)	1236:1326	The memory response to the LPS challenge was measured as changes in the production of inflammatory (TNFα, IL-6) and anti-inflammatory cytokines (IL-10, IL-1Ra), as compared to unprimed monocytes.
34421901	8	54	theme	rod-shaped	1573:1582	arg1	nanomaterials					1524:1536	nanomaterials	1524:1536	nanomaterials of different shapes (spherical vs. rod-shaped gold particles)	1524:1598	By comparing nanomaterials of different shapes (spherical vs. rod-shaped gold particles) and different size (17 vs. 22 nm diameter spherical iron oxide particles), it was evident that innate memory could be differentially induced and modulated depending on size, shape and chemical composition.
34421901	8	54	theme	rod-shaped	1573:1582	arg1	particles					1589:1597	spherical vs. rod-shaped gold particles	1559:1597	spherical vs. rod-shaped gold particles	1559:1597	By comparing nanomaterials of different shapes (spherical vs. rod-shaped gold particles) and different size (17 vs. 22 nm diameter spherical iron oxide particles), it was evident that innate memory could be differentially induced and modulated depending on size, shape and chemical composition.
34421901	1	55	theme	immune/inflammatory	270:288	arg1	effects					290:296	detrimental immune/inflammatory effects	258:296	detrimental immune/inflammatory effects	258:296	Engineered nanoparticles used for medical purposes must meet stringent safety criteria, which include immunosafety, i.e., the inability to activate possibly detrimental immune/inflammatory effects.
34421901	3	56	theme	innate	571:576	arg1	memory					578:583	innate memory	571:583	innate memory	571:583	Although innate memory is usually protective, anomalous innate memory responses may be at the basis of immune pathologies.
34421901	4	57	theme	innate	854:859	arg1	memory					861:866	innate memory	854:866	innate memory	854:866	In this study, we have examined the ability of two nanomaterials commonly used for diagnostic imaging purposes, gold and iron oxide nanoparticles, to induce or modulate innate memory, using an in vitro model based on human primary monocytes.
34421901	2	58	theme	Even	299:302	arg1	nanomaterials					312:324	Even medical nanomaterials	299:324	Even medical nanomaterials devoid of direct immunotoxic or inflammatory effects	299:377	Even medical nanomaterials devoid of direct immunotoxic or inflammatory effects may have an impact on human health if able to modify innate memory, which is the ability to "prime" future immune responses towards a different, possibly more detrimental reactivity.
34421901	2	59	theme	immunotoxic	343:353	arg1	effects					371:377	direct immunotoxic or inflammatory effects	336:377	direct immunotoxic or inflammatory effects	336:377	Even medical nanomaterials devoid of direct immunotoxic or inflammatory effects may have an impact on human health if able to modify innate memory, which is the ability to "prime" future immune responses towards a different, possibly more detrimental reactivity.
34421901	10	60	theme	imaging	2135:2141	arg1	nanoparticles					2143:2155	imaging nanoparticles	2135:2155	imaging nanoparticles	2135:2155	Thus, in order to predict the effect of imaging nanoparticles on the innate memory of patients, a personalised profiling would be required, able to take in consideration the peculiarities of the individual innate immune reactivity.
34421901	10	61	theme	reactivity	2315:2324	arg1	peculiarities					2269:2281	the peculiarities	2265:2281	the peculiarities of the individual innate immune reactivity	2265:2324	Thus, in order to predict the effect of imaging nanoparticles on the innate memory of patients, a personalised profiling would be required, able to take in consideration the peculiarities of the individual innate immune reactivity.
34421901	8	62	theme	chemical	1784:1791	arg1	composition					1793:1803	chemical composition	1784:1803	chemical composition	1784:1803	By comparing nanomaterials of different shapes (spherical vs. rod-shaped gold particles) and different size (17 vs. 22 nm diameter spherical iron oxide particles), it was evident that innate memory could be differentially induced and modulated depending on size, shape and chemical composition.
34421901	5	63	theme	phase/primary	1034:1046	arg1	LPS					1021:1023	the bacterial agent LPS	1001:1023	the bacterial agent LPS (priming phase/primary response)	1001:1056	Monocytes were exposed in culture to nanoparticles alone or together with the bacterial agent LPS (priming phase/primary response), then rested for six days (extinction phase), and eventually challenged with LPS (memory/secondary response).
34421901	5	63	theme	phase/primary	1034:1046	arg1	response					1048:1055	priming phase/primary response	1026:1055	priming phase/primary response	1026:1055	Monocytes were exposed in culture to nanoparticles alone or together with the bacterial agent LPS (priming phase/primary response), then rested for six days (extinction phase), and eventually challenged with LPS (memory/secondary response).
34421901	10	64	theme	innate	2164:2169	arg1	memory					2171:2176	the innate memory	2160:2176	the innate memory of patients	2160:2188	Thus, in order to predict the effect of imaging nanoparticles on the innate memory of patients, a personalised profiling would be required, able to take in consideration the peculiarities of the individual innate immune reactivity.
34421901	0	65	from	Particles	71:79	arg1	Monocytes					90:98	Human Monocytes	84:98	Human Monocytes	84:98	Personalised Profiling of Innate Immune Memory Induced by Nano-Imaging Particles in Human Monocytes.
34421901	6	66	theme	memory	1172:1177	arg1	changes					1225:1231	changes	1225:1231	changes in the production of inflammatory (TNFα, IL-6) and anti-inflammatory cytokines (IL-10, IL-1Ra)	1225:1326	The memory response to the LPS challenge was measured as changes in the production of inflammatory (TNFα, IL-6) and anti-inflammatory cytokines (IL-10, IL-1Ra), as compared to unprimed monocytes.
34421901	6	66	theme	memory	1172:1177	arg1	response					1179:1186	The memory response	1168:1186	The memory response to the LPS challenge	1168:1207	The memory response to the LPS challenge was measured as changes in the production of inflammatory (TNFα, IL-6) and anti-inflammatory cytokines (IL-10, IL-1Ra), as compared to unprimed monocytes.
34421901	9	67	from	donor	1938:1942	arg1	monocytes					1918:1926	monocytes	1918:1926	monocytes from each donor showing a distinct memory profile upon priming with the same particles	1918:2013	However, the main finding was that the innate memory effect of the particles was strongly donor-dependent, with monocytes from each donor showing a distinct memory profile upon priming with the same particles, thereby making impossible to draw general conclusions on the particle effects.
34421901	6	68	dep	TNFα	1268:1271	arg1	IL-6					1274:1277	IL-6	1274:1277	IL-6	1274:1277	The memory response to the LPS challenge was measured as changes in the production of inflammatory (TNFα, IL-6) and anti-inflammatory cytokines (IL-10, IL-1Ra), as compared to unprimed monocytes.
34421901	7	69	theme	cytokine	1490:1497	arg1	production					1499:1508	the cytokine production	1486:1508	the cytokine production	1486:1508	The results show that both types of nanoparticles can have an effect in the induction of memory, with changes observed in the cytokine production.
34421901	10	70	theme	personalised	2193:2204	arg1	profiling					2206:2214	a personalised profiling	2191:2214	a personalised profiling	2191:2214	Thus, in order to predict the effect of imaging nanoparticles on the innate memory of patients, a personalised profiling would be required, able to take in consideration the peculiarities of the individual innate immune reactivity.
34421901	9	71	theme	distinct	1954:1961	arg1	profile					1970:1976	a distinct memory profile	1952:1976	a distinct memory profile	1952:1976	However, the main finding was that the innate memory effect of the particles was strongly donor-dependent, with monocytes from each donor showing a distinct memory profile upon priming with the same particles, thereby making impossible to draw general conclusions on the particle effects.
34421901	5	72	theme	agent	1015:1019	arg1	LPS					1021:1023	the bacterial agent LPS	1001:1023	the bacterial agent LPS (priming phase/primary response)	1001:1056	Monocytes were exposed in culture to nanoparticles alone or together with the bacterial agent LPS (priming phase/primary response), then rested for six days (extinction phase), and eventually challenged with LPS (memory/secondary response).
34421901	5	72	theme	agent	1015:1019	arg1	response					1048:1055	priming phase/primary response	1026:1055	priming phase/primary response	1026:1055	Monocytes were exposed in culture to nanoparticles alone or together with the bacterial agent LPS (priming phase/primary response), then rested for six days (extinction phase), and eventually challenged with LPS (memory/secondary response).
34421901	3	73	theme	memory	625:630	arg1	responses					632:640	anomalous innate memory responses	608:640	anomalous innate memory responses	608:640	Although innate memory is usually protective, anomalous innate memory responses may be at the basis of immune pathologies.
34421901	8	74	theme	iron	1652:1655	arg1	particles					1663:1671	spherical iron oxide particles	1642:1671	17 vs. 22 nm diameter spherical iron oxide particles	1620:1671	By comparing nanomaterials of different shapes (spherical vs. rod-shaped gold particles) and different size (17 vs. 22 nm diameter spherical iron oxide particles), it was evident that innate memory could be differentially induced and modulated depending on size, shape and chemical composition.
34421901	1	75	theme	medical	135:141	arg1	purposes					143:150	medical purposes	135:150	medical purposes	135:150	Engineered nanoparticles used for medical purposes must meet stringent safety criteria, which include immunosafety, i.e., the inability to activate possibly detrimental immune/inflammatory effects.
34421901	9	76	theme	innate	1845:1850	arg1	effect					1859:1864	the innate memory effect	1841:1864	the innate memory effect of the particles	1841:1881	However, the main finding was that the innate memory effect of the particles was strongly donor-dependent, with monocytes from each donor showing a distinct memory profile upon priming with the same particles, thereby making impossible to draw general conclusions on the particle effects.
34421901	9	76	theme	innate	1845:1850	arg1	donor-dependent					1896:1910	donor-dependent	1896:1910	donor-dependent	1896:1910	However, the main finding was that the innate memory effect of the particles was strongly donor-dependent, with monocytes from each donor showing a distinct memory profile upon priming with the same particles, thereby making impossible to draw general conclusions on the particle effects.
34421901	4	77	dep	in	878:879	arg1	vitro					881:885	vitro	881:885	vitro	881:885	In this study, we have examined the ability of two nanomaterials commonly used for diagnostic imaging purposes, gold and iron oxide nanoparticles, to induce or modulate innate memory, using an in vitro model based on human primary monocytes.
34421901	3	78	theme	anomalous	608:616	arg1	responses					632:640	anomalous innate memory responses	608:640	anomalous innate memory responses	608:640	Although innate memory is usually protective, anomalous innate memory responses may be at the basis of immune pathologies.
34421901	2	79	theme	immune	486:491	arg1	responses					493:501	future immune responses	479:501	future immune responses	479:501	Even medical nanomaterials devoid of direct immunotoxic or inflammatory effects may have an impact on human health if able to modify innate memory, which is the ability to "prime" future immune responses towards a different, possibly more detrimental reactivity.
34421901	4	80	theme	human	902:906	arg1	monocytes					916:924	human primary monocytes	902:924	human primary monocytes	902:924	In this study, we have examined the ability of two nanomaterials commonly used for diagnostic imaging purposes, gold and iron oxide nanoparticles, to induce or modulate innate memory, using an in vitro model based on human primary monocytes.
34421901	0	81	theme	Human	84:88	arg1	Monocytes					90:98	Human Monocytes	84:98	Human Monocytes	84:98	Personalised Profiling of Innate Immune Memory Induced by Nano-Imaging Particles in Human Monocytes.
34421901	1	82	theme	stringent	162:170	arg1	criteria					179:186	stringent safety criteria	162:186	stringent safety criteria	162:186	Engineered nanoparticles used for medical purposes must meet stringent safety criteria, which include immunosafety, i.e., the inability to activate possibly detrimental immune/inflammatory effects.
34421901	8	83	dep	nm	1630:1631	arg1	22					1627:1628	22	1627:1628	22	1627:1628	By comparing nanomaterials of different shapes (spherical vs. rod-shaped gold particles) and different size (17 vs. 22 nm diameter spherical iron oxide particles), it was evident that innate memory could be differentially induced and modulated depending on size, shape and chemical composition.
34421901	8	83	dep	nm	1630:1631	arg1	17					1620:1621	17	1620:1621	17	1620:1621	By comparing nanomaterials of different shapes (spherical vs. rod-shaped gold particles) and different size (17 vs. 22 nm diameter spherical iron oxide particles), it was evident that innate memory could be differentially induced and modulated depending on size, shape and chemical composition.
34421901	6	84	theme	anti-inflammatory	1284:1300	arg1	IL-10					1313:1317	IL-10	1313:1317	IL-10	1313:1317	The memory response to the LPS challenge was measured as changes in the production of inflammatory (TNFα, IL-6) and anti-inflammatory cytokines (IL-10, IL-1Ra), as compared to unprimed monocytes.
34421901	6	84	theme	anti-inflammatory	1284:1300	arg1	cytokines					1302:1310	anti-inflammatory cytokines	1284:1310	anti-inflammatory cytokines (IL-10, IL-1Ra)	1284:1326	The memory response to the LPS challenge was measured as changes in the production of inflammatory (TNFα, IL-6) and anti-inflammatory cytokines (IL-10, IL-1Ra), as compared to unprimed monocytes.
34421901	5	85	theme	extinction	1085:1094	arg1	days					1079:1082	six days	1075:1082	six days (extinction phase)	1075:1101	Monocytes were exposed in culture to nanoparticles alone or together with the bacterial agent LPS (priming phase/primary response), then rested for six days (extinction phase), and eventually challenged with LPS (memory/secondary response).
34421901	5	85	theme	extinction	1085:1094	arg1	phase					1096:1100	extinction phase	1085:1100	extinction phase	1085:1100	Monocytes were exposed in culture to nanoparticles alone or together with the bacterial agent LPS (priming phase/primary response), then rested for six days (extinction phase), and eventually challenged with LPS (memory/secondary response).
34421901	2	86	theme	different	513:521	arg1	reactivity					550:559	a different, possibly more detrimental reactivity	511:559	a different, possibly more detrimental reactivity	511:559	Even medical nanomaterials devoid of direct immunotoxic or inflammatory effects may have an impact on human health if able to modify innate memory, which is the ability to "prime" future immune responses towards a different, possibly more detrimental reactivity.
34421901	4	87	theme	nanomaterials	736:748	arg1	ability					721:727	the ability	717:727	the ability of two nanomaterials commonly used for diagnostic imaging purposes, gold and iron oxide nanoparticles, to induce or modulate innate memory	717:866	In this study, we have examined the ability of two nanomaterials commonly used for diagnostic imaging purposes, gold and iron oxide nanoparticles, to induce or modulate innate memory, using an in vitro model based on human primary monocytes.
34421901	2	88	theme	human	401:405	arg1	health					407:412	human health	401:412	human health	401:412	Even medical nanomaterials devoid of direct immunotoxic or inflammatory effects may have an impact on human health if able to modify innate memory, which is the ability to "prime" future immune responses towards a different, possibly more detrimental reactivity.
34421901	0	89	theme	Innate	26:31	arg1	Memory					40:45	Innate Immune Memory	26:45	Innate Immune Memory	26:45	Personalised Profiling of Innate Immune Memory Induced by Nano-Imaging Particles in Human Monocytes.
34421901	1	90	theme	Engineered	101:110	arg1	nanoparticles					112:124	Engineered nanoparticles	101:124	Engineered nanoparticles used for medical purposes	101:150	Engineered nanoparticles used for medical purposes must meet stringent safety criteria, which include immunosafety, i.e., the inability to activate possibly detrimental immune/inflammatory effects.
34421901	2	91	theme	innate	432:437	arg1	ability					460:466	the ability to "prime" future immune responses towards a different, possibly more detrimental reactivity	456:559	the ability to "prime" future immune responses towards a different, possibly more detrimental reactivity	456:559	Even medical nanomaterials devoid of direct immunotoxic or inflammatory effects may have an impact on human health if able to modify innate memory, which is the ability to "prime" future immune responses towards a different, possibly more detrimental reactivity.
34421901	2	91	theme	innate	432:437	arg1	memory					439:444	innate memory	432:444	innate memory	432:444	Even medical nanomaterials devoid of direct immunotoxic or inflammatory effects may have an impact on human health if able to modify innate memory, which is the ability to "prime" future immune responses towards a different, possibly more detrimental reactivity.
34421901	0	92	theme	Memory	40:45	arg1	Profiling					13:21	Personalised Profiling	0:21	Personalised Profiling of Innate Immune Memory	0:45	Personalised Profiling of Innate Immune Memory Induced by Nano-Imaging Particles in Human Monocytes.
34421901	10	93	theme	innate	2301:2306	arg1	reactivity					2315:2324	the individual innate immune reactivity	2286:2324	the individual innate immune reactivity	2286:2324	Thus, in order to predict the effect of imaging nanoparticles on the innate memory of patients, a personalised profiling would be required, able to take in consideration the peculiarities of the individual innate immune reactivity.
34421901	3	94	theme	immune	665:670	arg1	pathologies					672:682	immune pathologies	665:682	immune pathologies	665:682	Although innate memory is usually protective, anomalous innate memory responses may be at the basis of immune pathologies.
34421901	4	95	theme	iron	806:809	arg1	nanoparticles					817:829	iron oxide nanoparticles	806:829	iron oxide nanoparticles	806:829	In this study, we have examined the ability of two nanomaterials commonly used for diagnostic imaging purposes, gold and iron oxide nanoparticles, to induce or modulate innate memory, using an in vitro model based on human primary monocytes.
34421901	4	95	theme	iron	806:809	arg1	nanomaterials					736:748	two nanomaterials	732:748	two nanomaterials commonly used for diagnostic imaging purposes	732:794	In this study, we have examined the ability of two nanomaterials commonly used for diagnostic imaging purposes, gold and iron oxide nanoparticles, to induce or modulate innate memory, using an in vitro model based on human primary monocytes.
34421901	2	96	theme	effects	371:377	arg1	devoid					326:331	devoid	326:331	devoid	326:331	Even medical nanomaterials devoid of direct immunotoxic or inflammatory effects may have an impact on human health if able to modify innate memory, which is the ability to "prime" future immune responses towards a different, possibly more detrimental reactivity.
34421901	9	97	theme	memory	1963:1968	arg1	profile					1970:1976	a distinct memory profile	1952:1976	a distinct memory profile	1952:1976	However, the main finding was that the innate memory effect of the particles was strongly donor-dependent, with monocytes from each donor showing a distinct memory profile upon priming with the same particles, thereby making impossible to draw general conclusions on the particle effects.
34421901	4	98	theme	diagnostic	768:777	arg1	purposes					787:794	diagnostic imaging purposes	768:794	diagnostic imaging purposes	768:794	In this study, we have examined the ability of two nanomaterials commonly used for diagnostic imaging purposes, gold and iron oxide nanoparticles, to induce or modulate innate memory, using an in vitro model based on human primary monocytes.
34421901	7	99	located	observed	1474:1481	arg1	production					1499:1508	the cytokine production	1486:1508	the cytokine production	1486:1508	The results show that both types of nanoparticles can have an effect in the induction of memory, with changes observed in the cytokine production.
34421901	7	99	located	observed	1474:1481	arg2	changes					1466:1472	changes	1466:1472	changes observed in the cytokine production	1466:1508	The results show that both types of nanoparticles can have an effect in the induction of memory, with changes observed in the cytokine production.
34421901	8	100	theme	gold	1584:1587	arg1	nanomaterials					1524:1536	nanomaterials	1524:1536	nanomaterials of different shapes (spherical vs. rod-shaped gold particles)	1524:1598	By comparing nanomaterials of different shapes (spherical vs. rod-shaped gold particles) and different size (17 vs. 22 nm diameter spherical iron oxide particles), it was evident that innate memory could be differentially induced and modulated depending on size, shape and chemical composition.
34421901	8	100	theme	gold	1584:1587	arg1	particles					1589:1597	spherical vs. rod-shaped gold particles	1559:1597	spherical vs. rod-shaped gold particles	1559:1597	By comparing nanomaterials of different shapes (spherical vs. rod-shaped gold particles) and different size (17 vs. 22 nm diameter spherical iron oxide particles), it was evident that innate memory could be differentially induced and modulated depending on size, shape and chemical composition.
31751198	11	0	theme	type	1470:1473	arg1	strain					1475:1480	the type strain	1466:1480	the type strain	1466:1480	nov. is proposed; the type strain is JSM 102062T (=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T).
31751198	11	0	theme	type	1470:1473	arg1	102062T					1489:1495	JSM 102062T	1485:1495	JSM 102062T (=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T)	1485:1556	nov. is proposed; the type strain is JSM 102062T (=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T).
31751198	8	1	theme	gene	882:885	arg1	sequences					887:895	16S rRNA gene sequences	873:895	16S rRNA gene sequences	873:895	Phylogeny based on 16S rRNA gene sequences indicated that strain JSM 102062T belonged to the genus Sediminibacillus, sharing high 16S rRNA gene sequence similarities to Sediminibacillus halophilus EN8dT (99.4 %) and Sediminibacillus albus NHBX5T (98.3 %).
31751198	2	2	from	Canyon	286:291	arg1	China					306:310	Hunan, PR China	296:310	China	306:310	A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium, strain JSM 102062T, was isolated from a non-saline farm soil sample collected from Dehang Canyon in Hunan, PR China.
31751198	2	3	attach	isolated	220:227	arg2	102062T					207:213	strain JSM 102062T	196:213	strain JSM 102062T	196:213	A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium, strain JSM 102062T, was isolated from a non-saline farm soil sample collected from Dehang Canyon in Hunan, PR China.
31751198	2	3	attach	isolated	220:227	arg1	sample					257:262	a non-saline farm soil sample	234:262	a non-saline farm soil sample collected from Dehang Canyon in Hunan, PR China	234:310	A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium, strain JSM 102062T, was isolated from a non-saline farm soil sample collected from Dehang Canyon in Hunan, PR China.
31751198	2	3	attach	isolated	220:227	arg2	bacterium					185:193	A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium	91:193	A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium	91:193	A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium, strain JSM 102062T, was isolated from a non-saline farm soil sample collected from Dehang Canyon in Hunan, PR China.
31751198	11	4	theme	=CCTCC AB	1498:1506	arg1	33541T					1550:1555	=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T	1498:1555	=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T	1498:1555	nov. is proposed; the type strain is JSM 102062T (=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T).
31751198	11	4	theme	=CCTCC AB	1498:1506	arg1	102062T					1489:1495	JSM 102062T	1485:1495	JSM 102062T (=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T)	1485:1556	nov. is proposed; the type strain is JSM 102062T (=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T).
31751198	8	5	theme	genus	947:951	arg1	Sediminibacillus					953:968	the genus Sediminibacillus	943:968	the genus Sediminibacillus	943:968	Phylogeny based on 16S rRNA gene sequences indicated that strain JSM 102062T belonged to the genus Sediminibacillus, sharing high 16S rRNA gene sequence similarities to Sediminibacillus halophilus EN8dT (99.4 %) and Sediminibacillus albus NHBX5T (98.3 %).
31751198	3	6	theme	optimum	419:425	arg1	20-50 °C					409:416	20-50 °C	409:416	20-50 °C (optimum 30-35 °C)	409:435	Growth occurred with 0.5-20 % (w/v) NaCl (optimum 4-7 %) at pH 5.5-11.0 (optimum pH 8.0) and at 20-50 °C (optimum 30-35 °C).
31751198	3	6	theme	optimum	419:425	arg1	30-35 °C					427:434	optimum 30-35 °C	419:434	optimum 30-35 °C	419:434	Growth occurred with 0.5-20 % (w/v) NaCl (optimum 4-7 %) at pH 5.5-11.0 (optimum pH 8.0) and at 20-50 °C (optimum 30-35 °C).
31751198	3	7	theme	0.5-20 	334:340	arg1	NaCl					349:352	0.5-20 % (w/v) NaCl	334:352	0.5-20 % (w/v) NaCl (optimum 4-7 %)	334:368	Growth occurred with 0.5-20 % (w/v) NaCl (optimum 4-7 %) at pH 5.5-11.0 (optimum pH 8.0) and at 20-50 °C (optimum 30-35 °C).
31751198	3	7	theme	0.5-20 	334:340	arg1	%					367:367	optimum 4-7 %	355:367	optimum 4-7 %	355:367	Growth occurred with 0.5-20 % (w/v) NaCl (optimum 4-7 %) at pH 5.5-11.0 (optimum pH 8.0) and at 20-50 °C (optimum 30-35 °C).
31751198	2	8	theme	Gram-stain-positive	93:111	arg1	bacterium					185:193	A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium	91:193	A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium	91:193	A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium, strain JSM 102062T, was isolated from a non-saline farm soil sample collected from Dehang Canyon in Hunan, PR China.
31751198	2	8	theme	Gram-stain-positive	93:111	arg1	102062T					207:213	strain JSM 102062T	196:213	strain JSM 102062T	196:213	A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium, strain JSM 102062T, was isolated from a non-saline farm soil sample collected from Dehang Canyon in Hunan, PR China.
31751198	5	9	theme	major	590:594	arg1	anteiso-C15 					622:633	anteiso-C15 	622:633	anteiso-C15 	622:633	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
31751198	5	9	theme	major	590:594	arg1	acids					611:615	The major cellular fatty acids	586:615	The major cellular fatty acids	586:615	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
31751198	9	10	theme	different	1182:1190	arg1	taxon					1192:1196	a different taxon	1180:1196	a different taxon separated from the recognized Sediminibacillus species	1180:1251	The whole genomic analysis showed that strain JSM 102062T constituted a different taxon separated from the recognized Sediminibacillus species.
31751198	2	11	theme	Dehang	279:284	arg1	Canyon					286:291	Dehang Canyon	279:291	Dehang Canyon in Hunan, PR China	279:310	A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium, strain JSM 102062T, was isolated from a non-saline farm soil sample collected from Dehang Canyon in Hunan, PR China.
31751198	10	12	theme	genotypic	1288:1296	arg1	studies					1298:1304	phenotypic and genotypic studies	1273:1304	phenotypic and genotypic studies	1273:1304	Combined data from phenotypic and genotypic studies demonstrated that strain JSM 102062T represents a noval species of the genus Sediminibacillus, for which the name Sediminibacillus terrae sp.
31751198	9	13	theme	recognized	1217:1226	arg1	species					1245:1251	the recognized Sediminibacillus species	1213:1251	the recognized Sediminibacillus species	1213:1251	The whole genomic analysis showed that strain JSM 102062T constituted a different taxon separated from the recognized Sediminibacillus species.
31751198	3	14	theme	%	341:341	arg1	NaCl					349:352	0.5-20 % (w/v) NaCl	334:352	0.5-20 % (w/v) NaCl (optimum 4-7 %)	334:368	Growth occurred with 0.5-20 % (w/v) NaCl (optimum 4-7 %) at pH 5.5-11.0 (optimum pH 8.0) and at 20-50 °C (optimum 30-35 °C).
31751198	3	14	theme	%	341:341	arg1	%					367:367	optimum 4-7 %	355:367	optimum 4-7 %	355:367	Growth occurred with 0.5-20 % (w/v) NaCl (optimum 4-7 %) at pH 5.5-11.0 (optimum pH 8.0) and at 20-50 °C (optimum 30-35 °C).
31751198	9	15	theme	Sediminibacillus	1228:1243	arg1	species					1245:1251	the recognized Sediminibacillus species	1213:1251	the recognized Sediminibacillus species	1213:1251	The whole genomic analysis showed that strain JSM 102062T constituted a different taxon separated from the recognized Sediminibacillus species.
31751198	5	16	theme	cellular	596:603	arg1	anteiso-C15 					622:633	anteiso-C15 	622:633	anteiso-C15 	622:633	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
31751198	5	16	theme	cellular	596:603	arg1	acids					611:615	The major cellular fatty acids	586:615	The major cellular fatty acids	586:615	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
31751198	8	17	theme	JSM	919:921	arg1	102062T					923:929	strain JSM 102062T	912:929	strain JSM 102062T	912:929	Phylogeny based on 16S rRNA gene sequences indicated that strain JSM 102062T belonged to the genus Sediminibacillus, sharing high 16S rRNA gene sequence similarities to Sediminibacillus halophilus EN8dT (99.4 %) and Sediminibacillus albus NHBX5T (98.3 %).
31751198	8	18	theme	16S	984:986	arg1	similarities					1007:1018	high 16S rRNA gene sequence similarities	979:1018	high 16S rRNA gene sequence similarities to Sediminibacillus halophilus EN8dT (99.4 %) and Sediminibacillus albus NHBX5T (98.3 %)	979:1107	Phylogeny based on 16S rRNA gene sequences indicated that strain JSM 102062T belonged to the genus Sediminibacillus, sharing high 16S rRNA gene sequence similarities to Sediminibacillus halophilus EN8dT (99.4 %) and Sediminibacillus albus NHBX5T (98.3 %).
31751198	7	19	theme	G+C	827:829	arg1	content					831:837	The DNA G+C content	819:837	The DNA G+C content	819:837	The DNA G+C content was 44.1 mol%.
31751198	7	19	theme	G+C	827:829	arg1	%					851:851	44.1 mol%	843:851	44.1 mol%	843:851	The DNA G+C content was 44.1 mol%.
31751198	10	20	theme	Sediminibacillus	1383:1398	arg1	species					1362:1368	a noval species	1354:1368	a noval species	1354:1368	Combined data from phenotypic and genotypic studies demonstrated that strain JSM 102062T represents a noval species of the genus Sediminibacillus, for which the name Sediminibacillus terrae sp.
31751198	8	21	theme	high	979:982	arg1	similarities					1007:1018	high 16S rRNA gene sequence similarities	979:1018	high 16S rRNA gene sequence similarities to Sediminibacillus halophilus EN8dT (99.4 %) and Sediminibacillus albus NHBX5T (98.3 %)	979:1107	Phylogeny based on 16S rRNA gene sequences indicated that strain JSM 102062T belonged to the genus Sediminibacillus, sharing high 16S rRNA gene sequence similarities to Sediminibacillus halophilus EN8dT (99.4 %) and Sediminibacillus albus NHBX5T (98.3 %).
31751198	9	22	theme	whole	1114:1118	arg1	analysis					1128:1135	The whole genomic analysis	1110:1135	The whole genomic analysis	1110:1135	The whole genomic analysis showed that strain JSM 102062T constituted a different taxon separated from the recognized Sediminibacillus species.
31751198	1	23	theme	non-saline	69:78	arg1	soil					85:88	non-saline farm soil	69:88	non-saline farm soil	69:88	nov., a moderate halophile isolated from non-saline farm soil.
31751198	0	24	theme	terrae	17:22	arg1	sp					24:25	Sediminibacillus terrae sp	0:25	Sediminibacillus terrae sp.	0:26	Sediminibacillus terrae sp.
31751198	8	25	theme	gene	993:996	arg1	similarities					1007:1018	high 16S rRNA gene sequence similarities	979:1018	high 16S rRNA gene sequence similarities to Sediminibacillus halophilus EN8dT (99.4 %) and Sediminibacillus albus NHBX5T (98.3 %)	979:1107	Phylogeny based on 16S rRNA gene sequences indicated that strain JSM 102062T belonged to the genus Sediminibacillus, sharing high 16S rRNA gene sequence similarities to Sediminibacillus halophilus EN8dT (99.4 %) and Sediminibacillus albus NHBX5T (98.3 %).
31751198	9	26	theme	genomic	1120:1126	arg1	analysis					1128:1135	The whole genomic analysis	1110:1135	The whole genomic analysis	1110:1135	The whole genomic analysis showed that strain JSM 102062T constituted a different taxon separated from the recognized Sediminibacillus species.
31751198	1	27	theme	farm	80:83	arg1	soil					85:88	non-saline farm soil	69:88	non-saline farm soil	69:88	nov., a moderate halophile isolated from non-saline farm soil.
31751198	2	28	dep	China	306:310	arg1	PR					303:304	Hunan, PR China	296:310	PR	303:304	A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium, strain JSM 102062T, was isolated from a non-saline farm soil sample collected from Dehang Canyon in Hunan, PR China.
31751198	0	29	theme	Sediminibacillus	0:15	arg1	sp					24:25	Sediminibacillus terrae sp	0:25	Sediminibacillus terrae sp.	0:26	Sediminibacillus terrae sp.
31751198	10	30	from	studies	1298:1304	arg1	data					1263:1266	Combined data	1254:1266	Combined data from phenotypic and genotypic studies	1254:1304	Combined data from phenotypic and genotypic studies demonstrated that strain JSM 102062T represents a noval species of the genus Sediminibacillus, for which the name Sediminibacillus terrae sp.
31751198	8	31	theme	rRNA	988:991	arg1	similarities					1007:1018	high 16S rRNA gene sequence similarities	979:1018	high 16S rRNA gene sequence similarities to Sediminibacillus halophilus EN8dT (99.4 %) and Sediminibacillus albus NHBX5T (98.3 %)	979:1107	Phylogeny based on 16S rRNA gene sequences indicated that strain JSM 102062T belonged to the genus Sediminibacillus, sharing high 16S rRNA gene sequence similarities to Sediminibacillus halophilus EN8dT (99.4 %) and Sediminibacillus albus NHBX5T (98.3 %).
31751198	6	32	theme	lipid	682:686	arg1	pattern					688:694	The polar lipid pattern	672:694	The polar lipid pattern	672:694	The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, five unidentified phospholipids and an unidentified glycolipid.
31751198	2	33	theme	Hunan	296:300	arg1	China					306:310	Hunan, PR China	296:310	China	306:310	A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium, strain JSM 102062T, was isolated from a non-saline farm soil sample collected from Dehang Canyon in Hunan, PR China.
31751198	10	34	theme	Combined	1254:1261	arg1	data					1263:1266	Combined data	1254:1266	Combined data from phenotypic and genotypic studies	1254:1304	Combined data from phenotypic and genotypic studies demonstrated that strain JSM 102062T represents a noval species of the genus Sediminibacillus, for which the name Sediminibacillus terrae sp.
31751198	9	35	theme	strain	1149:1154	arg1	102062T					1160:1166	strain JSM 102062T	1149:1166	strain JSM 102062T	1149:1166	The whole genomic analysis showed that strain JSM 102062T constituted a different taxon separated from the recognized Sediminibacillus species.
31751198	8	36	dep	halophilus	1040:1049	arg1	NHBX5T					1093:1098	NHBX5T	1093:1098	NHBX5T	1093:1098	Phylogeny based on 16S rRNA gene sequences indicated that strain JSM 102062T belonged to the genus Sediminibacillus, sharing high 16S rRNA gene sequence similarities to Sediminibacillus halophilus EN8dT (99.4 %) and Sediminibacillus albus NHBX5T (98.3 %).
31751198	6	37	theme	polar	676:680	arg1	pattern					688:694	The polar lipid pattern	672:694	The polar lipid pattern	672:694	The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, five unidentified phospholipids and an unidentified glycolipid.
31751198	11	38	theme	JSM	1485:1487	arg1	strain					1475:1480	the type strain	1466:1480	the type strain	1466:1480	nov. is proposed; the type strain is JSM 102062T (=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T).
31751198	11	38	theme	JSM	1485:1487	arg1	102062T					1489:1495	JSM 102062T	1485:1495	JSM 102062T (=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T)	1485:1556	nov. is proposed; the type strain is JSM 102062T (=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T).
31751198	11	38	theme	JSM	1485:1487	arg1	33541T					1550:1555	=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T	1498:1555	=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T	1498:1555	nov. is proposed; the type strain is JSM 102062T (=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T).
31751198	4	39	theme	respiratory	554:564	arg1	menaquinone-7					520:532	menaquinone-7	520:532	menaquinone-7 (MK-7)	520:539	Contained cell-wall peptidoglycan based on meso-diaminopimelic acid and possessed menaquinone-7 (MK-7) as the major respiratory isoprenoid quinone.
31751198	4	39	theme	respiratory	554:564	arg1	quinone					577:583	the major respiratory isoprenoid quinone	544:583	the major respiratory isoprenoid quinone	544:583	Contained cell-wall peptidoglycan based on meso-diaminopimelic acid and possessed menaquinone-7 (MK-7) as the major respiratory isoprenoid quinone.
31751198	3	40	theme	w/v	344:346	arg1	NaCl					349:352	0.5-20 % (w/v) NaCl	334:352	0.5-20 % (w/v) NaCl (optimum 4-7 %)	334:368	Growth occurred with 0.5-20 % (w/v) NaCl (optimum 4-7 %) at pH 5.5-11.0 (optimum pH 8.0) and at 20-50 °C (optimum 30-35 °C).
31751198	3	40	theme	w/v	344:346	arg1	%					367:367	optimum 4-7 %	355:367	optimum 4-7 %	355:367	Growth occurred with 0.5-20 % (w/v) NaCl (optimum 4-7 %) at pH 5.5-11.0 (optimum pH 8.0) and at 20-50 °C (optimum 30-35 °C).
31751198	10	41	dep	Sediminibacillus	1420:1435	arg1	terrae					1437:1442	terrae	1437:1442	terrae	1437:1442	Combined data from phenotypic and genotypic studies demonstrated that strain JSM 102062T represents a noval species of the genus Sediminibacillus, for which the name Sediminibacillus terrae sp.
31751198	8	42	theme	sequence	998:1005	arg1	similarities					1007:1018	high 16S rRNA gene sequence similarities	979:1018	high 16S rRNA gene sequence similarities to Sediminibacillus halophilus EN8dT (99.4 %) and Sediminibacillus albus NHBX5T (98.3 %)	979:1107	Phylogeny based on 16S rRNA gene sequences indicated that strain JSM 102062T belonged to the genus Sediminibacillus, sharing high 16S rRNA gene sequence similarities to Sediminibacillus halophilus EN8dT (99.4 %) and Sediminibacillus albus NHBX5T (98.3 %).
31751198	4	43	theme	major	548:552	arg1	menaquinone-7					520:532	menaquinone-7	520:532	menaquinone-7 (MK-7)	520:539	Contained cell-wall peptidoglycan based on meso-diaminopimelic acid and possessed menaquinone-7 (MK-7) as the major respiratory isoprenoid quinone.
31751198	4	43	theme	major	548:552	arg1	quinone					577:583	the major respiratory isoprenoid quinone	544:583	the major respiratory isoprenoid quinone	544:583	Contained cell-wall peptidoglycan based on meso-diaminopimelic acid and possessed menaquinone-7 (MK-7) as the major respiratory isoprenoid quinone.
31751198	3	44	theme	4-7 	363:366	arg1	NaCl					349:352	0.5-20 % (w/v) NaCl	334:352	0.5-20 % (w/v) NaCl (optimum 4-7 %)	334:368	Growth occurred with 0.5-20 % (w/v) NaCl (optimum 4-7 %) at pH 5.5-11.0 (optimum pH 8.0) and at 20-50 °C (optimum 30-35 °C).
31751198	3	44	theme	4-7 	363:366	arg1	%					367:367	optimum 4-7 %	355:367	optimum 4-7 %	355:367	Growth occurred with 0.5-20 % (w/v) NaCl (optimum 4-7 %) at pH 5.5-11.0 (optimum pH 8.0) and at 20-50 °C (optimum 30-35 °C).
31751198	4	45	theme	meso-diaminopimelic	481:499	arg1	acid					501:504	meso-diaminopimelic acid	481:504	meso-diaminopimelic acid	481:504	Contained cell-wall peptidoglycan based on meso-diaminopimelic acid and possessed menaquinone-7 (MK-7) as the major respiratory isoprenoid quinone.
31751198	6	46	theme	unidentified	794:805	arg1	glycolipid					807:816	an unidentified glycolipid	791:816	an unidentified glycolipid	791:816	The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, five unidentified phospholipids and an unidentified glycolipid.
31751198	10	47	theme	Sediminibacillus	1420:1435	arg1	sp					1444:1445	the name Sediminibacillus terrae sp	1411:1445	the name Sediminibacillus terrae sp	1411:1445	Combined data from phenotypic and genotypic studies demonstrated that strain JSM 102062T represents a noval species of the genus Sediminibacillus, for which the name Sediminibacillus terrae sp.
31751198	9	48	theme	JSM	1156:1158	arg1	102062T					1160:1166	strain JSM 102062T	1149:1166	strain JSM 102062T	1149:1166	The whole genomic analysis showed that strain JSM 102062T constituted a different taxon separated from the recognized Sediminibacillus species.
31751198	11	49	theme	CGMCC	1519:1523	arg1	33541T					1550:1555	=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T	1498:1555	=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T	1498:1555	nov. is proposed; the type strain is JSM 102062T (=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T).
31751198	11	49	theme	CGMCC	1519:1523	arg1	102062T					1489:1495	JSM 102062T	1485:1495	JSM 102062T (=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T)	1485:1556	nov. is proposed; the type strain is JSM 102062T (=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T).
31751198	3	50	theme	optimum	386:392	arg1	pH					373:374	pH 5.5-11.0	373:383	pH 5.5-11.0 (optimum pH 8.0)	373:400	Growth occurred with 0.5-20 % (w/v) NaCl (optimum 4-7 %) at pH 5.5-11.0 (optimum pH 8.0) and at 20-50 °C (optimum 30-35 °C).
31751198	3	50	theme	optimum	386:392	arg1	pH					394:395	optimum pH 8.0	386:399	optimum pH 8.0	386:399	Growth occurred with 0.5-20 % (w/v) NaCl (optimum 4-7 %) at pH 5.5-11.0 (optimum pH 8.0) and at 20-50 °C (optimum 30-35 °C).
31751198	2	51	dep	Gram-stain-positive	93:111	arg1	rod-shaped					174:183	rod-shaped	174:183	rod-shaped	174:183	A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium, strain JSM 102062T, was isolated from a non-saline farm soil sample collected from Dehang Canyon in Hunan, PR China.
31751198	2	51	dep	Gram-stain-positive	93:111	arg1	endospore-forming					155:171	endospore-forming	155:171	endospore-forming	155:171	A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium, strain JSM 102062T, was isolated from a non-saline farm soil sample collected from Dehang Canyon in Hunan, PR China.
31751198	2	51	dep	Gram-stain-positive	93:111	arg1	halophilic					125:134	halophilic	125:134	halophilic	125:134	A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium, strain JSM 102062T, was isolated from a non-saline farm soil sample collected from Dehang Canyon in Hunan, PR China.
31751198	2	51	dep	Gram-stain-positive	93:111	arg1	aerobic					146:152	aerobic	146:152	aerobic	146:152	A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium, strain JSM 102062T, was isolated from a non-saline farm soil sample collected from Dehang Canyon in Hunan, PR China.
31751198	5	52	theme	fatty	605:609	arg1	anteiso-C15 					622:633	anteiso-C15 	622:633	anteiso-C15 	622:633	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
31751198	5	52	theme	fatty	605:609	arg1	acids					611:615	The major cellular fatty acids	586:615	The major cellular fatty acids	586:615	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
31751198	11	53	theme	1.12957T=DSM	1525:1536	arg1	33541T					1550:1555	=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T	1498:1555	=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T	1498:1555	nov. is proposed; the type strain is JSM 102062T (=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T).
31751198	11	53	theme	1.12957T=DSM	1525:1536	arg1	102062T					1489:1495	JSM 102062T	1485:1495	JSM 102062T (=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T)	1485:1556	nov. is proposed; the type strain is JSM 102062T (=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T).
31751198	10	54	theme	phenotypic	1273:1282	arg1	studies					1298:1304	phenotypic and genotypic studies	1273:1304	phenotypic and genotypic studies	1273:1304	Combined data from phenotypic and genotypic studies demonstrated that strain JSM 102062T represents a noval species of the genus Sediminibacillus, for which the name Sediminibacillus terrae sp.
31751198	3	55	theme	optimum	355:361	arg1	NaCl					349:352	0.5-20 % (w/v) NaCl	334:352	0.5-20 % (w/v) NaCl (optimum 4-7 %)	334:368	Growth occurred with 0.5-20 % (w/v) NaCl (optimum 4-7 %) at pH 5.5-11.0 (optimum pH 8.0) and at 20-50 °C (optimum 30-35 °C).
31751198	3	55	theme	optimum	355:361	arg1	%					367:367	optimum 4-7 %	355:367	optimum 4-7 %	355:367	Growth occurred with 0.5-20 % (w/v) NaCl (optimum 4-7 %) at pH 5.5-11.0 (optimum pH 8.0) and at 20-50 °C (optimum 30-35 °C).
31751198	6	56	theme	unidentified	760:771	arg1	phospholipids					773:785	five unidentified phospholipids	755:785	five unidentified phospholipids	755:785	The polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, five unidentified phospholipids and an unidentified glycolipid.
31751198	11	57	theme	2014166T	1508:1515	arg1	33541T					1550:1555	=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T	1498:1555	=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T	1498:1555	nov. is proposed; the type strain is JSM 102062T (=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T).
31751198	11	57	theme	2014166T	1508:1515	arg1	102062T					1489:1495	JSM 102062T	1485:1495	JSM 102062T (=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T)	1485:1556	nov. is proposed; the type strain is JSM 102062T (=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T).
31751198	10	58	theme	genus	1377:1381	arg1	Sediminibacillus					1383:1398	the genus Sediminibacillus	1373:1398	the genus Sediminibacillus	1373:1398	Combined data from phenotypic and genotypic studies demonstrated that strain JSM 102062T represents a noval species of the genus Sediminibacillus, for which the name Sediminibacillus terrae sp.
31751198	7	59	theme	44.1 mol	843:850	arg1	content					831:837	The DNA G+C content	819:837	The DNA G+C content	819:837	The DNA G+C content was 44.1 mol%.
31751198	7	59	theme	44.1 mol	843:850	arg1	%					851:851	44.1 mol%	843:851	44.1 mol%	843:851	The DNA G+C content was 44.1 mol%.
31751198	4	60	theme	cell-wall	448:456	arg1	peptidoglycan					458:470	cell-wall peptidoglycan	448:470	cell-wall peptidoglycan	448:470	Contained cell-wall peptidoglycan based on meso-diaminopimelic acid and possessed menaquinone-7 (MK-7) as the major respiratory isoprenoid quinone.
31751198	2	61	theme	JSM	203:205	arg1	bacterium					185:193	A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium	91:193	A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium	91:193	A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium, strain JSM 102062T, was isolated from a non-saline farm soil sample collected from Dehang Canyon in Hunan, PR China.
31751198	2	61	theme	JSM	203:205	arg1	102062T					207:213	strain JSM 102062T	196:213	strain JSM 102062T	196:213	A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium, strain JSM 102062T, was isolated from a non-saline farm soil sample collected from Dehang Canyon in Hunan, PR China.
31751198	2	62	theme	soil	252:255	arg1	sample					257:262	a non-saline farm soil sample	234:262	a non-saline farm soil sample collected from Dehang Canyon in Hunan, PR China	234:310	A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium, strain JSM 102062T, was isolated from a non-saline farm soil sample collected from Dehang Canyon in Hunan, PR China.
31751198	11	63	theme	=	1517:1517	arg1	33541T					1550:1555	=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T	1498:1555	=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T	1498:1555	nov. is proposed; the type strain is JSM 102062T (=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T).
31751198	11	63	theme	=	1517:1517	arg1	102062T					1489:1495	JSM 102062T	1485:1495	JSM 102062T (=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T)	1485:1556	nov. is proposed; the type strain is JSM 102062T (=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T).
31751198	8	64	theme	strain	912:917	arg1	102062T					923:929	strain JSM 102062T	912:929	strain JSM 102062T	912:929	Phylogeny based on 16S rRNA gene sequences indicated that strain JSM 102062T belonged to the genus Sediminibacillus, sharing high 16S rRNA gene sequence similarities to Sediminibacillus halophilus EN8dT (99.4 %) and Sediminibacillus albus NHBX5T (98.3 %).
31751198	2	65	theme	strain	196:201	arg1	bacterium					185:193	A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium	91:193	A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium	91:193	A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium, strain JSM 102062T, was isolated from a non-saline farm soil sample collected from Dehang Canyon in Hunan, PR China.
31751198	2	65	theme	strain	196:201	arg1	102062T					207:213	strain JSM 102062T	196:213	strain JSM 102062T	196:213	A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium, strain JSM 102062T, was isolated from a non-saline farm soil sample collected from Dehang Canyon in Hunan, PR China.
31751198	2	66	theme	farm	247:250	arg1	sample					257:262	a non-saline farm soil sample	234:262	a non-saline farm soil sample collected from Dehang Canyon in Hunan, PR China	234:310	A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium, strain JSM 102062T, was isolated from a non-saline farm soil sample collected from Dehang Canyon in Hunan, PR China.
31751198	8	67	theme	rRNA	877:880	arg1	sequences					887:895	16S rRNA gene sequences	873:895	16S rRNA gene sequences	873:895	Phylogeny based on 16S rRNA gene sequences indicated that strain JSM 102062T belonged to the genus Sediminibacillus, sharing high 16S rRNA gene sequence similarities to Sediminibacillus halophilus EN8dT (99.4 %) and Sediminibacillus albus NHBX5T (98.3 %).
31751198	10	68	theme	noval	1356:1360	arg1	species					1362:1368	a noval species	1354:1368	a noval species	1354:1368	Combined data from phenotypic and genotypic studies demonstrated that strain JSM 102062T represents a noval species of the genus Sediminibacillus, for which the name Sediminibacillus terrae sp.
31751198	4	69	theme	isoprenoid	566:575	arg1	menaquinone-7					520:532	menaquinone-7	520:532	menaquinone-7 (MK-7)	520:539	Contained cell-wall peptidoglycan based on meso-diaminopimelic acid and possessed menaquinone-7 (MK-7) as the major respiratory isoprenoid quinone.
31751198	4	69	theme	isoprenoid	566:575	arg1	quinone					577:583	the major respiratory isoprenoid quinone	544:583	the major respiratory isoprenoid quinone	544:583	Contained cell-wall peptidoglycan based on meso-diaminopimelic acid and possessed menaquinone-7 (MK-7) as the major respiratory isoprenoid quinone.
31751198	2	70	theme	non-saline	236:245	arg1	sample					257:262	a non-saline farm soil sample	234:262	a non-saline farm soil sample collected from Dehang Canyon in Hunan, PR China	234:310	A Gram-stain-positive, moderately halophilic, strictly aerobic, endospore-forming, rod-shaped bacterium, strain JSM 102062T, was isolated from a non-saline farm soil sample collected from Dehang Canyon in Hunan, PR China.
31751198	8	71	theme	16S	873:875	arg1	sequences					887:895	16S rRNA gene sequences	873:895	16S rRNA gene sequences	873:895	Phylogeny based on 16S rRNA gene sequences indicated that strain JSM 102062T belonged to the genus Sediminibacillus, sharing high 16S rRNA gene sequence similarities to Sediminibacillus halophilus EN8dT (99.4 %) and Sediminibacillus albus NHBX5T (98.3 %).
31751198	10	72	theme	JSM	1331:1333	arg1	102062T					1335:1341	strain JSM 102062T	1324:1341	strain JSM 102062T	1324:1341	Combined data from phenotypic and genotypic studies demonstrated that strain JSM 102062T represents a noval species of the genus Sediminibacillus, for which the name Sediminibacillus terrae sp.
31751198	10	73	theme	strain	1324:1329	arg1	102062T					1335:1341	strain JSM 102062T	1324:1341	strain JSM 102062T	1324:1341	Combined data from phenotypic and genotypic studies demonstrated that strain JSM 102062T represents a noval species of the genus Sediminibacillus, for which the name Sediminibacillus terrae sp.
31751198	10	74	theme	name	1415:1418	arg1	sp					1444:1445	the name Sediminibacillus terrae sp	1411:1445	the name Sediminibacillus terrae sp	1411:1445	Combined data from phenotypic and genotypic studies demonstrated that strain JSM 102062T represents a noval species of the genus Sediminibacillus, for which the name Sediminibacillus terrae sp.
31751198	1	75	theme	moderate	36:43	arg1	halophile					45:53	a moderate halophile	34:53	a moderate halophile	34:53	nov., a moderate halophile isolated from non-saline farm soil.
31751198	1	75	theme	moderate	36:43	arg1	nov.					28:31	nov.	28:31	nov.	28:31	nov., a moderate halophile isolated from non-saline farm soil.
31751198	11	76	theme	28949T=KCTC	1538:1548	arg1	33541T					1550:1555	=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T	1498:1555	=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T	1498:1555	nov. is proposed; the type strain is JSM 102062T (=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T).
31751198	11	76	theme	28949T=KCTC	1538:1548	arg1	102062T					1489:1495	JSM 102062T	1485:1495	JSM 102062T (=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T)	1485:1556	nov. is proposed; the type strain is JSM 102062T (=CCTCC AB 2014166T = CGMCC 1.12957T=DSM 28949T=KCTC 33541T).
31751198	7	77	theme	DNA	823:825	arg1	content					831:837	The DNA G+C content	819:837	The DNA G+C content	819:837	The DNA G+C content was 44.1 mol%.
31751198	7	77	theme	DNA	823:825	arg1	%					851:851	44.1 mol%	843:851	44.1 mol%	843:851	The DNA G+C content was 44.1 mol%.
31751198	5	78	dep	anteiso-C15 	622:633	arg1	 0					668:669	 0	668:669	 0	668:669	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
31751198	5	78	dep	anteiso-C15 	622:633	arg1	iso-C16 					659:666	iso-C16 	659:666	iso-C16 	659:666	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
31751198	5	78	dep	anteiso-C15 	622:633	arg1	 0					652:653	 0	652:653	 0	652:653	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
31751198	5	78	dep	anteiso-C15 	622:633	arg1	 0					635:636	 0	635:636	 0	635:636	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0.
34196604	6	0	theme	chemotaxonomic	869:882	arg1	data					884:887	morphological and chemotaxonomic data	851:887	morphological and chemotaxonomic data	851:887	A combination of morphological and chemotaxonomic data supported the assignment to the genus Streptomyces.
34196604	13	1	theme	type	1701:1704	arg1	CH5-8T					1716:1721	CH5-8T	1716:1721	CH5-8T (=TBRC 9950T=NBRC 113997T)	1716:1748	The type strain is CH5-8T (=TBRC 9950T=NBRC 113997T).
34196604	13	1	theme	type	1701:1704	arg1	strain					1706:1711	The type strain	1697:1711	The type strain	1697:1711	The type strain is CH5-8T (=TBRC 9950T=NBRC 113997T).
34196604	4	2	from	acid	486:489	arg1	H6					529:530	H6	529:530	H6	529:530	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	4	2	from	acid	486:489	arg1	H4					543:544	H4	543:544	H4	543:544	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	4	2	from	acid	486:489	arg1	H8					519:520	H8	519:520	H8	519:520	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	4	2	from	acid	486:489	arg1	MK-9					538:541	MK-9	538:541	MK-9(H4)	538:545	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	4	2	from	acid	486:489	arg1	peptidoglycan					499:511	cell peptidoglycan	494:511	cell peptidoglycan	494:511	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	4	2	from	acid	486:489	arg1	MK-9					524:527	MK-9	524:527	MK-9(H6)	524:531	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	4	2	from	acid	486:489	arg1	MK-9					514:517	MK-9	514:517	MK-9(H8)	514:521	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	8	3	theme	16S	1115:1117	arg1	rRNA					1119:1122	the highest 16S rRNA gene sequence values	1103:1143	the highest 16S rRNA gene sequence values	1103:1143	The novel strain CH5-8T showed the highest 16S rRNA gene sequence values to Streptomyces echinatus NBRC 12763T (98.9 %), followed by Streptomyces actinomycinicus RCU-197T (98.9 %).
34196604	6	4	theme	morphological	851:863	arg1	data					884:887	morphological and chemotaxonomic data	851:887	morphological and chemotaxonomic data	851:887	A combination of morphological and chemotaxonomic data supported the assignment to the genus Streptomyces.
34196604	10	5	theme	digital	1485:1491	arg1	content					1501:1507	The digital DNA G+C content	1481:1507	The digital DNA G+C content of genomic DNA	1481:1522	The digital DNA G+C content of genomic DNA was 72.1 mol%.
34196604	10	5	theme	digital	1485:1491	arg1	%					1536:1536	72.1 mol%	1528:1536	72.1 mol%	1528:1536	The digital DNA G+C content of genomic DNA was 72.1 mol%.
34196604	2	6	theme	spore	133:137	arg1	chains					123:128	spiral chains	116:128	spiral chains of spore arising from the aerial mycelium	116:170	An actinobacterium, strain CH5-8T, which formed spiral chains of spore arising from the aerial mycelium, was isolated from rhizosphere soil of Musa spp.
34196604	8	7	theme	gene	1124:1127	arg1	values					1138:1143	gene sequence values	1124:1143	the highest 16S rRNA gene sequence values	1103:1143	The novel strain CH5-8T showed the highest 16S rRNA gene sequence values to Streptomyces echinatus NBRC 12763T (98.9 %), followed by Streptomyces actinomycinicus RCU-197T (98.9 %).
34196604	4	8	theme	chemotaxonomic	367:380	arg1	anteiso-C15 					573:584	anteiso-C15 	573:584	anteiso-C15 	573:584	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	4	8	theme	chemotaxonomic	367:380	arg1	menaquinones					556:567	major menaquinones	550:567	major menaquinones	550:567	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	4	8	theme	chemotaxonomic	367:380	arg1	properties					382:391	The typical chemotaxonomic properties	355:391	The typical chemotaxonomic properties of members of the genus Streptomyces	355:428	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	9	9	theme	S.	1384:1385	arg1	3313T					1402:1406	S. echinatus CECT 3313T	1384:1406	S. echinatus CECT 3313T	1384:1406	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6 % and ≤47.4 %, respectively.
34196604	9	9	theme	S.	1384:1385	arg1	relatives					1373:1381	its closest relatives	1361:1381	its closest relatives	1361:1381	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6 % and ≤47.4 %, respectively.
34196604	8	10	theme	Streptomyces	1148:1159	arg1	12763T					1176:1181	Streptomyces echinatus NBRC 12763T	1148:1181	Streptomyces echinatus NBRC 12763T (98.9 %)	1148:1190	The novel strain CH5-8T showed the highest 16S rRNA gene sequence values to Streptomyces echinatus NBRC 12763T (98.9 %), followed by Streptomyces actinomycinicus RCU-197T (98.9 %).
34196604	8	10	theme	Streptomyces	1148:1159	arg1	%					1189:1189	98.9 %	1184:1189	98.9 %	1184:1189	The novel strain CH5-8T showed the highest 16S rRNA gene sequence values to Streptomyces echinatus NBRC 12763T (98.9 %), followed by Streptomyces actinomycinicus RCU-197T (98.9 %).
34196604	3	11	theme	vivid	244:248	arg1	mycelium					276:283	vivid greenish yellow substrate mycelium	244:283	vivid greenish yellow substrate mycelium	244:283	The organism exhibited vivid greenish yellow substrate mycelium and easily produced the medium grey aerial spore mass on ISP2 medium.
34196604	10	12	theme	G+C	1497:1499	arg1	content					1501:1507	The digital DNA G+C content	1481:1507	The digital DNA G+C content of genomic DNA	1481:1522	The digital DNA G+C content of genomic DNA was 72.1 mol%.
34196604	10	12	theme	G+C	1497:1499	arg1	%					1536:1536	72.1 mol%	1528:1536	72.1 mol%	1528:1536	The digital DNA G+C content of genomic DNA was 72.1 mol%.
34196604	5	13	theme	phosphatidylinositol	775:794	arg1	mannoside					796:804	phosphatidylinositol mannoside	775:804	phosphatidylinositol mannoside	775:804	Diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside were detected in the cells.
34196604	8	14	theme	NBRC	1171:1174	arg1	12763T					1176:1181	Streptomyces echinatus NBRC 12763T	1148:1181	Streptomyces echinatus NBRC 12763T (98.9 %)	1148:1190	The novel strain CH5-8T showed the highest 16S rRNA gene sequence values to Streptomyces echinatus NBRC 12763T (98.9 %), followed by Streptomyces actinomycinicus RCU-197T (98.9 %).
34196604	8	14	theme	NBRC	1171:1174	arg1	%					1189:1189	98.9 %	1184:1189	98.9 %	1184:1189	The novel strain CH5-8T showed the highest 16S rRNA gene sequence values to Streptomyces echinatus NBRC 12763T (98.9 %), followed by Streptomyces actinomycinicus RCU-197T (98.9 %).
34196604	4	15	theme	genus	411:415	arg1	Streptomyces					417:428	the genus Streptomyces	407:428	the genus Streptomyces	407:428	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	4	16	theme	major	550:554	arg1	menaquinones					556:567	major menaquinones	550:567	major menaquinones	550:567	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	4	16	theme	major	550:554	arg1	properties					382:391	The typical chemotaxonomic properties	355:391	The typical chemotaxonomic properties of members of the genus Streptomyces	355:428	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	10	17	theme	72.1 mol	1528:1535	arg1	content					1501:1507	The digital DNA G+C content	1481:1507	The digital DNA G+C content of genomic DNA	1481:1522	The digital DNA G+C content of genomic DNA was 72.1 mol%.
34196604	10	17	theme	72.1 mol	1528:1535	arg1	%					1536:1536	72.1 mol%	1528:1536	72.1 mol%	1528:1536	The digital DNA G+C content of genomic DNA was 72.1 mol%.
34196604	7	18	theme	16S	978:980	arg1	rRNA					982:985	16S rRNA	978:985	the 16S rRNA gene sequence	974:999	The analysis result obtained for the 16S rRNA gene sequence confirmed the taxonomic affiliation at the genus level of this strain.
34196604	10	19	theme	DNA	1520:1522	arg1	content					1501:1507	The digital DNA G+C content	1481:1507	The digital DNA G+C content of genomic DNA	1481:1522	The digital DNA G+C content of genomic DNA was 72.1 mol%.
34196604	10	19	theme	DNA	1520:1522	arg1	%					1536:1536	72.1 mol%	1528:1536	72.1 mol%	1528:1536	The digital DNA G+C content of genomic DNA was 72.1 mol%.
34196604	3	20	theme	yellow	259:264	arg1	mycelium					276:283	vivid greenish yellow substrate mycelium	244:283	vivid greenish yellow substrate mycelium	244:283	The organism exhibited vivid greenish yellow substrate mycelium and easily produced the medium grey aerial spore mass on ISP2 medium.
34196604	2	21	theme	Musa	211:214	arg1	spp					216:218	Musa spp	211:218	Musa spp	211:218	An actinobacterium, strain CH5-8T, which formed spiral chains of spore arising from the aerial mycelium, was isolated from rhizosphere soil of Musa spp.
34196604	11	22	theme	phenotypic	1561:1570	arg1	data					1586:1589	these phenotypic and genotypic data	1555:1589	these phenotypic and genotypic data	1555:1589	On the basis of these phenotypic and genotypic data, strain CH5-8T represents a novel species, for which the name Streptomyces musisoli sp.
34196604	11	23	theme	Streptomyces	1653:1664	arg1	sp					1675:1676	the name Streptomyces musisoli sp	1644:1676	the name Streptomyces musisoli sp	1644:1676	On the basis of these phenotypic and genotypic data, strain CH5-8T represents a novel species, for which the name Streptomyces musisoli sp.
34196604	9	24	theme	 and	1453:1456	arg1	%					1464:1464	≤91.6 % and ≤47.4 %	1446:1464	≤91.6 % and ≤47.4 %	1446:1464	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6 % and ≤47.4 %, respectively.
34196604	9	24	theme	 and	1453:1456	arg1	identity					1276:1283	The average nucleotide identity	1253:1283	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T,	1253:1439	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6 % and ≤47.4 %, respectively.
34196604	3	25	theme	spore	328:332	arg1	mass					334:337	the medium grey aerial spore mass	305:337	the medium grey aerial spore mass	305:337	The organism exhibited vivid greenish yellow substrate mycelium and easily produced the medium grey aerial spore mass on ISP2 medium.
34196604	8	26	dep	Streptomyces	1205:1216	arg1	actinomycinicus					1218:1232	actinomycinicus	1218:1232	actinomycinicus	1218:1232	The novel strain CH5-8T showed the highest 16S rRNA gene sequence values to Streptomyces echinatus NBRC 12763T (98.9 %), followed by Streptomyces actinomycinicus RCU-197T (98.9 %).
34196604	3	27	theme	grey	316:319	arg1	mass					334:337	the medium grey aerial spore mass	305:337	the medium grey aerial spore mass	305:337	The organism exhibited vivid greenish yellow substrate mycelium and easily produced the medium grey aerial spore mass on ISP2 medium.
34196604	9	28	dep	S.	1412:1413	arg1	actinomycinicus					1415:1429	actinomycinicus	1415:1429	actinomycinicus	1415:1429	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6 % and ≤47.4 %, respectively.
34196604	13	29	theme	9950T=NBRC	1730:1739	arg1	CH5-8T					1716:1721	CH5-8T	1716:1721	CH5-8T (=TBRC 9950T=NBRC 113997T)	1716:1748	The type strain is CH5-8T (=TBRC 9950T=NBRC 113997T).
34196604	13	29	theme	9950T=NBRC	1730:1739	arg1	113997T					1741:1747	=TBRC 9950T=NBRC 113997T	1724:1747	=TBRC 9950T=NBRC 113997T	1724:1747	The type strain is CH5-8T (=TBRC 9950T=NBRC 113997T).
34196604	2	30	attach	isolated	177:184	arg1	soil					203:206	rhizosphere soil	191:206	rhizosphere soil of Musa spp	191:218	An actinobacterium, strain CH5-8T, which formed spiral chains of spore arising from the aerial mycelium, was isolated from rhizosphere soil of Musa spp.
34196604	2	30	attach	isolated	177:184	arg2	strain					88:93	strain CH5-8T	88:100	strain CH5-8T	88:100	An actinobacterium, strain CH5-8T, which formed spiral chains of spore arising from the aerial mycelium, was isolated from rhizosphere soil of Musa spp.
34196604	2	30	attach	isolated	177:184	arg2	actinobacterium					71:85	An actinobacterium	68:85	An actinobacterium	68:85	An actinobacterium, strain CH5-8T, which formed spiral chains of spore arising from the aerial mycelium, was isolated from rhizosphere soil of Musa spp.
34196604	0	31	theme	Streptomyces	0:11	arg1	sp					22:23	Streptomyces musisoli sp	0:23	Streptomyces musisoli sp.	0:24	Streptomyces musisoli sp.
34196604	9	32	theme	nucleotide	1265:1274	arg1	identity					1276:1283	The average nucleotide identity	1253:1283	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T,	1253:1439	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6 % and ≤47.4 %, respectively.
34196604	9	32	theme	nucleotide	1265:1274	arg1	%					1464:1464	≤91.6 % and ≤47.4 %	1446:1464	≤91.6 % and ≤47.4 %	1446:1464	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6 % and ≤47.4 %, respectively.
34196604	4	33	theme	major	626:630	arg1	acids					638:642	major fatty acids	626:642	major fatty acids	626:642	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	7	34	dep	analysis	945:952	arg1	result					954:959	result	954:959	result obtained for the 16S rRNA gene sequence	954:999	The analysis result obtained for the 16S rRNA gene sequence confirmed the taxonomic affiliation at the genus level of this strain.
34196604	11	35	theme	strain	1592:1597	arg1	CH5-8T					1599:1604	strain CH5-8T	1592:1604	strain CH5-8T	1592:1604	On the basis of these phenotypic and genotypic data, strain CH5-8T represents a novel species, for which the name Streptomyces musisoli sp.
34196604	4	36	theme	cell	494:497	arg1	peptidoglycan					499:511	cell peptidoglycan	494:511	cell peptidoglycan	494:511	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	8	37	dep	Streptomyces	1148:1159	arg1	echinatus					1161:1169	echinatus	1161:1169	echinatus	1161:1169	The novel strain CH5-8T showed the highest 16S rRNA gene sequence values to Streptomyces echinatus NBRC 12763T (98.9 %), followed by Streptomyces actinomycinicus RCU-197T (98.9 %).
34196604	11	38	theme	novel	1619:1623	arg1	species					1625:1631	a novel species	1617:1631	a novel species	1617:1631	On the basis of these phenotypic and genotypic data, strain CH5-8T represents a novel species, for which the name Streptomyces musisoli sp.
34196604	9	39	theme	digital	1305:1311	arg1	hybridization					1321:1333	digital DNA-DNA hybridization	1305:1333	digital DNA-DNA hybridization	1305:1333	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6 % and ≤47.4 %, respectively.
34196604	9	40	theme	hybridization	1321:1333	arg1	values					1335:1340	blast (ANIb) and digital DNA-DNA hybridization values	1288:1340	blast (ANIb) and digital DNA-DNA hybridization values	1288:1340	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6 % and ≤47.4 %, respectively.
34196604	8	41	theme	strain	1082:1087	arg1	CH5-8T					1089:1094	The novel strain CH5-8T	1072:1094	The novel strain CH5-8T	1072:1094	The novel strain CH5-8T showed the highest 16S rRNA gene sequence values to Streptomyces echinatus NBRC 12763T (98.9 %), followed by Streptomyces actinomycinicus RCU-197T (98.9 %).
34196604	6	42	theme	data	884:887	arg1	combination					836:846	A combination	834:846	A combination of morphological and chemotaxonomic data	834:887	A combination of morphological and chemotaxonomic data supported the assignment to the genus Streptomyces.
34196604	4	43	dep	observed	435:442	arg1	 0					586:587	 0	586:587	 0	586:587	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	4	43	dep	observed	435:442	arg1	 0					620:621	 0	620:621	 0 as major fatty acids	620:642	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	4	43	dep	observed	435:442	arg1	 0					599:600	 0	599:600	 0	599:600	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	4	43	dep	observed	435:442	arg1	anteiso-C17 					607:618	anteiso-C17 	607:618	anteiso-C17 	607:618	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	4	44	theme	typical	359:365	arg1	anteiso-C15 					573:584	anteiso-C15 	573:584	anteiso-C15 	573:584	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	4	44	theme	typical	359:365	arg1	menaquinones					556:567	major menaquinones	550:567	major menaquinones	550:567	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	4	44	theme	typical	359:365	arg1	properties					382:391	The typical chemotaxonomic properties	355:391	The typical chemotaxonomic properties of members of the genus Streptomyces	355:428	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	10	45	theme	DNA	1493:1495	arg1	content					1501:1507	The digital DNA G+C content	1481:1507	The digital DNA G+C content of genomic DNA	1481:1522	The digital DNA G+C content of genomic DNA was 72.1 mol%.
34196604	10	45	theme	DNA	1493:1495	arg1	%					1536:1536	72.1 mol%	1528:1536	72.1 mol%	1528:1536	The digital DNA G+C content of genomic DNA was 72.1 mol%.
34196604	7	46	theme	strain	1064:1069	arg1	level					1050:1054	the genus level	1040:1054	the genus level of this strain	1040:1069	The analysis result obtained for the 16S rRNA gene sequence confirmed the taxonomic affiliation at the genus level of this strain.
34196604	8	47	theme	highest	1107:1113	arg1	rRNA					1119:1122	the highest 16S rRNA gene sequence values	1103:1143	the highest 16S rRNA gene sequence values	1103:1143	The novel strain CH5-8T showed the highest 16S rRNA gene sequence values to Streptomyces echinatus NBRC 12763T (98.9 %), followed by Streptomyces actinomycinicus RCU-197T (98.9 %).
34196604	9	48	theme	closest	1365:1371	arg1	RCU-197T					1431:1438	S. actinomycinicus RCU-197T	1412:1438	S. actinomycinicus RCU-197T	1412:1438	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6 % and ≤47.4 %, respectively.
34196604	9	48	theme	closest	1365:1371	arg1	3313T					1402:1406	S. echinatus CECT 3313T	1384:1406	S. echinatus CECT 3313T	1384:1406	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6 % and ≤47.4 %, respectively.
34196604	9	48	theme	closest	1365:1371	arg1	relatives					1373:1381	its closest relatives	1361:1381	its closest relatives	1361:1381	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6 % and ≤47.4 %, respectively.
34196604	10	49	theme	genomic	1512:1518	arg1	DNA					1520:1522	genomic DNA	1512:1522	genomic DNA	1512:1522	The digital DNA G+C content of genomic DNA was 72.1 mol%.
34196604	2	50	theme	spiral	116:121	arg1	chains					123:128	spiral chains	116:128	spiral chains of spore arising from the aerial mycelium	116:170	An actinobacterium, strain CH5-8T, which formed spiral chains of spore arising from the aerial mycelium, was isolated from rhizosphere soil of Musa spp.
34196604	7	51	theme	genus	1044:1048	arg1	level					1050:1054	the genus level	1040:1054	the genus level of this strain	1040:1069	The analysis result obtained for the 16S rRNA gene sequence confirmed the taxonomic affiliation at the genus level of this strain.
34196604	8	52	theme	sequence	1129:1136	arg1	values					1138:1143	gene sequence values	1124:1143	the highest 16S rRNA gene sequence values	1103:1143	The novel strain CH5-8T showed the highest 16S rRNA gene sequence values to Streptomyces echinatus NBRC 12763T (98.9 %), followed by Streptomyces actinomycinicus RCU-197T (98.9 %).
34196604	1	53	attach	isolated	48:55	arg2	actinomycete					35:46	an actinomycete	32:46	an actinomycete isolated from soil	32:65	nov., an actinomycete isolated from soil.
34196604	1	53	attach	isolated	48:55	arg1	soil					62:65	soil	62:65	soil	62:65	nov., an actinomycete isolated from soil.
34196604	9	54	theme	echinatus	1387:1395	arg1	3313T					1402:1406	S. echinatus CECT 3313T	1384:1406	S. echinatus CECT 3313T	1384:1406	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6 % and ≤47.4 %, respectively.
34196604	9	54	theme	echinatus	1387:1395	arg1	relatives					1373:1381	its closest relatives	1361:1381	its closest relatives	1361:1381	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6 % and ≤47.4 %, respectively.
34196604	6	55	theme	genus	921:925	arg1	Streptomyces					927:938	the genus Streptomyces	917:938	the genus Streptomyces	917:938	A combination of morphological and chemotaxonomic data supported the assignment to the genus Streptomyces.
34196604	3	56	theme	greenish	250:257	arg1	mycelium					276:283	vivid greenish yellow substrate mycelium	244:283	vivid greenish yellow substrate mycelium	244:283	The organism exhibited vivid greenish yellow substrate mycelium and easily produced the medium grey aerial spore mass on ISP2 medium.
34196604	4	57	theme	Streptomyces	417:428	arg1	members					396:402	members	396:402	members of the genus Streptomyces	396:428	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	1	58	dep	actinomycete	35:46	arg1	nov.					26:29	nov.	26:29	nov.	26:29	nov., an actinomycete isolated from soil.
34196604	7	59	theme	taxonomic	1015:1023	arg1	affiliation					1025:1035	the taxonomic affiliation	1011:1035	the taxonomic affiliation	1011:1035	The analysis result obtained for the 16S rRNA gene sequence confirmed the taxonomic affiliation at the genus level of this strain.
34196604	5	60	located	detected	811:818	arg2	phosphatidylinositol					750:769	phosphatidylinositol	750:769	phosphatidylinositol	750:769	Diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside were detected in the cells.
34196604	5	60	located	detected	811:818	arg2	phosphatidylethanolamine					669:692	phosphatidylethanolamine	669:692	phosphatidylethanolamine	669:692	Diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside were detected in the cells.
34196604	5	60	located	detected	811:818	arg2	hydroxyphosphatidylethanolamine					695:725	hydroxyphosphatidylethanolamine	695:725	hydroxyphosphatidylethanolamine	695:725	Diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside were detected in the cells.
34196604	5	60	located	detected	811:818	arg2	Diphosphatidylglycerol					645:666	Diphosphatidylglycerol	645:666	Diphosphatidylglycerol	645:666	Diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside were detected in the cells.
34196604	5	60	located	detected	811:818	arg2	phosphatidylglycerol					728:747	phosphatidylglycerol	728:747	phosphatidylglycerol	728:747	Diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside were detected in the cells.
34196604	5	60	located	detected	811:818	arg2	mannoside					796:804	phosphatidylinositol mannoside	775:804	phosphatidylinositol mannoside	775:804	Diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside were detected in the cells.
34196604	5	60	located	detected	811:818	arg1	cells					827:831	the cells	823:831	the cells	823:831	Diphosphatidylglycerol, phosphatidylethanolamine, hydroxyphosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside were detected in the cells.
34196604	8	61	theme	Streptomyces	1205:1216	arg1	RCU-197T					1234:1241	Streptomyces actinomycinicus RCU-197T	1205:1241	Streptomyces actinomycinicus RCU-197T (98.9 %)	1205:1250	The novel strain CH5-8T showed the highest 16S rRNA gene sequence values to Streptomyces echinatus NBRC 12763T (98.9 %), followed by Streptomyces actinomycinicus RCU-197T (98.9 %).
34196604	8	61	theme	Streptomyces	1205:1216	arg1	%					1249:1249	98.9 %	1244:1249	98.9 %	1244:1249	The novel strain CH5-8T showed the highest 16S rRNA gene sequence values to Streptomyces echinatus NBRC 12763T (98.9 %), followed by Streptomyces actinomycinicus RCU-197T (98.9 %).
34196604	2	62	theme	rhizosphere	191:201	arg1	soil					203:206	rhizosphere soil	191:206	rhizosphere soil of Musa spp	191:218	An actinobacterium, strain CH5-8T, which formed spiral chains of spore arising from the aerial mycelium, was isolated from rhizosphere soil of Musa spp.
34196604	7	63	theme	rRNA	982:985	arg1	sequence					992:999	the 16S rRNA gene sequence	974:999	the 16S rRNA gene sequence	974:999	The analysis result obtained for the 16S rRNA gene sequence confirmed the taxonomic affiliation at the genus level of this strain.
34196604	9	64	theme	S.	1412:1413	arg1	RCU-197T					1431:1438	S. actinomycinicus RCU-197T	1412:1438	S. actinomycinicus RCU-197T	1412:1438	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6 % and ≤47.4 %, respectively.
34196604	9	64	theme	S.	1412:1413	arg1	relatives					1373:1381	its closest relatives	1361:1381	its closest relatives	1361:1381	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6 % and ≤47.4 %, respectively.
34196604	4	65	theme	members	396:402	arg1	anteiso-C15 					573:584	anteiso-C15 	573:584	anteiso-C15 	573:584	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	4	65	theme	members	396:402	arg1	menaquinones					556:567	major menaquinones	550:567	major menaquinones	550:567	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	4	65	theme	members	396:402	arg1	properties					382:391	The typical chemotaxonomic properties	355:391	The typical chemotaxonomic properties of members of the genus Streptomyces	355:428	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	7	66	theme	gene	987:990	arg1	sequence					992:999	the 16S rRNA gene sequence	974:999	the 16S rRNA gene sequence	974:999	The analysis result obtained for the 16S rRNA gene sequence confirmed the taxonomic affiliation at the genus level of this strain.
34196604	3	67	theme	substrate	266:274	arg1	mycelium					276:283	vivid greenish yellow substrate mycelium	244:283	vivid greenish yellow substrate mycelium	244:283	The organism exhibited vivid greenish yellow substrate mycelium and easily produced the medium grey aerial spore mass on ISP2 medium.
34196604	2	68	theme	spp	216:218	arg1	soil					203:206	rhizosphere soil	191:206	rhizosphere soil of Musa spp	191:218	An actinobacterium, strain CH5-8T, which formed spiral chains of spore arising from the aerial mycelium, was isolated from rhizosphere soil of Musa spp.
34196604	3	69	theme	medium	309:314	arg1	mass					334:337	the medium grey aerial spore mass	305:337	the medium grey aerial spore mass	305:337	The organism exhibited vivid greenish yellow substrate mycelium and easily produced the medium grey aerial spore mass on ISP2 medium.
34196604	4	70	theme	ll-diaminopimelic	468:484	arg1	acid					486:489	ll-diaminopimelic acid	468:489	ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4)	468:545	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	9	71	theme	%	1452:1452	arg1	%					1464:1464	≤91.6 % and ≤47.4 %	1446:1464	≤91.6 % and ≤47.4 %	1446:1464	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6 % and ≤47.4 %, respectively.
34196604	9	71	theme	%	1452:1452	arg1	identity					1276:1283	The average nucleotide identity	1253:1283	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T,	1253:1439	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6 % and ≤47.4 %, respectively.
34196604	8	72	dep	rRNA	1119:1122	arg1	values					1138:1143	gene sequence values	1124:1143	the highest 16S rRNA gene sequence values	1103:1143	The novel strain CH5-8T showed the highest 16S rRNA gene sequence values to Streptomyces echinatus NBRC 12763T (98.9 %), followed by Streptomyces actinomycinicus RCU-197T (98.9 %).
34196604	11	73	theme	musisoli	1666:1673	arg1	sp					1675:1676	the name Streptomyces musisoli sp	1644:1676	the name Streptomyces musisoli sp	1644:1676	On the basis of these phenotypic and genotypic data, strain CH5-8T represents a novel species, for which the name Streptomyces musisoli sp.
34196604	9	74	theme	≤47.4 	1458:1463	arg1	%					1464:1464	≤91.6 % and ≤47.4 %	1446:1464	≤91.6 % and ≤47.4 %	1446:1464	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6 % and ≤47.4 %, respectively.
34196604	9	74	theme	≤47.4 	1458:1463	arg1	identity					1276:1283	The average nucleotide identity	1253:1283	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T,	1253:1439	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6 % and ≤47.4 %, respectively.
34196604	4	75	theme	strain	448:453	arg1	CH5-8T					455:460	strain CH5-8T	448:460	strain CH5-8T	448:460	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	4	76	theme	fatty	632:636	arg1	acids					638:642	major fatty acids	626:642	major fatty acids	626:642	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	13	77	theme	=TBRC	1724:1728	arg1	CH5-8T					1716:1721	CH5-8T	1716:1721	CH5-8T (=TBRC 9950T=NBRC 113997T)	1716:1748	The type strain is CH5-8T (=TBRC 9950T=NBRC 113997T).
34196604	13	77	theme	=TBRC	1724:1728	arg1	113997T					1741:1747	=TBRC 9950T=NBRC 113997T	1724:1747	=TBRC 9950T=NBRC 113997T	1724:1747	The type strain is CH5-8T (=TBRC 9950T=NBRC 113997T).
34196604	11	78	theme	name	1648:1651	arg1	sp					1675:1676	the name Streptomyces musisoli sp	1644:1676	the name Streptomyces musisoli sp	1644:1676	On the basis of these phenotypic and genotypic data, strain CH5-8T represents a novel species, for which the name Streptomyces musisoli sp.
34196604	9	79	theme	CECT	1397:1400	arg1	3313T					1402:1406	S. echinatus CECT 3313T	1384:1406	S. echinatus CECT 3313T	1384:1406	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6 % and ≤47.4 %, respectively.
34196604	9	79	theme	CECT	1397:1400	arg1	relatives					1373:1381	its closest relatives	1361:1381	its closest relatives	1361:1381	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6 % and ≤47.4 %, respectively.
34196604	3	80	theme	aerial	321:326	arg1	mass					334:337	the medium grey aerial spore mass	305:337	the medium grey aerial spore mass	305:337	The organism exhibited vivid greenish yellow substrate mycelium and easily produced the medium grey aerial spore mass on ISP2 medium.
34196604	11	81	dep	data	1586:1589	arg1	the					1542:1544	the	1542:1544	the	1542:1544	On the basis of these phenotypic and genotypic data, strain CH5-8T represents a novel species, for which the name Streptomyces musisoli sp.
34196604	11	81	dep	data	1586:1589	arg1	basis					1546:1550	basis	1546:1550	basis	1546:1550	On the basis of these phenotypic and genotypic data, strain CH5-8T represents a novel species, for which the name Streptomyces musisoli sp.
34196604	0	82	theme	musisoli	13:20	arg1	sp					22:23	Streptomyces musisoli sp	0:23	Streptomyces musisoli sp.	0:24	Streptomyces musisoli sp.
34196604	9	83	theme	average	1257:1263	arg1	identity					1276:1283	The average nucleotide identity	1253:1283	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T,	1253:1439	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6 % and ≤47.4 %, respectively.
34196604	9	83	theme	average	1257:1263	arg1	%					1464:1464	≤91.6 % and ≤47.4 %	1446:1464	≤91.6 % and ≤47.4 %	1446:1464	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6 % and ≤47.4 %, respectively.
34196604	3	84	theme	ISP2	342:345	arg1	medium					347:352	ISP2 medium	342:352	ISP2 medium	342:352	The organism exhibited vivid greenish yellow substrate mycelium and easily produced the medium grey aerial spore mass on ISP2 medium.
34196604	2	85	theme	aerial	156:161	arg1	mycelium					163:170	the aerial mycelium	152:170	the aerial mycelium	152:170	An actinobacterium, strain CH5-8T, which formed spiral chains of spore arising from the aerial mycelium, was isolated from rhizosphere soil of Musa spp.
34196604	7	86	dep	result	954:959	arg1	obtained					961:968	obtained	961:968	result obtained for the 16S rRNA gene sequence	954:999	The analysis result obtained for the 16S rRNA gene sequence confirmed the taxonomic affiliation at the genus level of this strain.
34196604	9	87	theme	blast	1288:1292	arg1	values					1335:1340	blast (ANIb) and digital DNA-DNA hybridization values	1288:1340	blast (ANIb) and digital DNA-DNA hybridization values	1288:1340	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6 % and ≤47.4 %, respectively.
34196604	11	88	theme	genotypic	1576:1584	arg1	data					1586:1589	these phenotypic and genotypic data	1555:1589	these phenotypic and genotypic data	1555:1589	On the basis of these phenotypic and genotypic data, strain CH5-8T represents a novel species, for which the name Streptomyces musisoli sp.
34196604	4	89	dep	acid	486:489	arg1	e.g.					463:466	e.g.	463:466	e.g.	463:466	The typical chemotaxonomic properties of members of the genus Streptomyces were observed for strain CH5-8T, e.g. ll-diaminopimelic acid in cell peptidoglycan, MK-9(H8), MK-9(H6), and MK-9(H4) as major menaquinones and anteiso-C15 : 0, iso-C16 : 0, and anteiso-C17 : 0 as major fatty acids.
34196604	8	90	theme	novel	1076:1080	arg1	CH5-8T					1089:1094	The novel strain CH5-8T	1072:1094	The novel strain CH5-8T	1072:1094	The novel strain CH5-8T showed the highest 16S rRNA gene sequence values to Streptomyces echinatus NBRC 12763T (98.9 %), followed by Streptomyces actinomycinicus RCU-197T (98.9 %).
34196604	9	91	theme	DNA-DNA	1313:1319	arg1	hybridization					1321:1333	digital DNA-DNA hybridization	1305:1333	digital DNA-DNA hybridization	1305:1333	The average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization values between CH5-8T and its closest relatives, S. echinatus CECT 3313T and S. actinomycinicus RCU-197T, were ≤91.6 % and ≤47.4 %, respectively.
34139131	3	0	theme	WEAN7	692:696	arg1	cohort					698:703	WEAN7 cohort	692:703	WEAN7 cohort	692:703	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	3	1	theme	Rats	560:563	arg1	function					409:416	contractile function	397:416	contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort)	397:825	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	8	2	theme	rat	1548:1550	arg1	model					1552:1556	a rat model	1546:1556	a rat model of diet-induced obesity	1546:1580	Novelty: We assessed contractile function of permeabilized skeletal muscle fibres in a rat model of diet-induced obesity.
34139131	8	3	theme	skeletal	1520:1527	arg1	fibres					1536:1541	permeabilized skeletal muscle fibres	1506:1541	permeabilized skeletal muscle fibres	1506:1541	Novelty: We assessed contractile function of permeabilized skeletal muscle fibres in a rat model of diet-induced obesity.
34139131	5	4	from	fibres	1071:1076	arg1	cohort					1093:1098	the ADULT24 cohort	1081:1098	the ADULT24 cohort	1081:1098	Relative to CHOW diet groups, the HFHS diet was associated with impaired force production in (a) MHC I fibres in the ADULT24 cohort; and (b) MHC IIa fibres in the ADULT12 and ADULT24 cohorts combined.
34139131	5	4	from	fibres	1071:1076	arg1	cohorts					1151:1157	the ADULT12 and ADULT24 cohorts	1127:1157	cohorts	1151:1157	Relative to CHOW diet groups, the HFHS diet was associated with impaired force production in (a) MHC I fibres in the ADULT24 cohort; and (b) MHC IIa fibres in the ADULT12 and ADULT24 cohorts combined.
34139131	2	5	theme	muscle	285:290	arg1	function					292:299	muscle function	285:299	muscle function observed with obesity	285:321	It is not clear if contractile protein dysfunction contributes to the impairment of muscle function observed with obesity.
34139131	5	6	dep	fibres	1117:1122	arg1	b					1106:1106	b	1106:1106	b	1106:1106	Relative to CHOW diet groups, the HFHS diet was associated with impaired force production in (a) MHC I fibres in the ADULT24 cohort; and (b) MHC IIa fibres in the ADULT12 and ADULT24 cohorts combined.
34139131	3	7	theme	14-week	709:715	arg1	duration					717:724	14-week duration	709:724	14-week duration	709:724	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	3	8	theme	high-fat	583:590	arg1	beginning					644:652	a standard (CHOW) diet beginning	621:652	a standard (CHOW) diet beginning	621:652	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	3	8	theme	high-fat	583:590	arg1	diet					613:616	a high-fat, high sucrose (HFHS) diet	581:616	diet	613:616	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	3	8	theme	high-fat	583:590	arg1	weanlings					664:672	either weanlings	657:672	either weanlings	657:672	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	8	9	theme	obesity	1574:1580	arg1	model					1552:1556	a rat model	1546:1556	a rat model of diet-induced obesity	1546:1580	Novelty: We assessed contractile function of permeabilized skeletal muscle fibres in a rat model of diet-induced obesity.
34139131	2	10	theme	contractile	220:230	arg1	dysfunction					240:250	contractile protein dysfunction	220:250	contractile protein dysfunction	220:250	It is not clear if contractile protein dysfunction contributes to the impairment of muscle function observed with obesity.
34139131	9	11	dep	diet	1610:1613	arg1	high-sucrose					1597:1608	The high-fat, high-sucrose diet	1583:1613	high-sucrose	1597:1608	The high-fat, high-sucrose diet was associated with impaired force output of fibres expressing MHC I or MHC IIa in some cohorts of rats.
34139131	5	12	theme	CHOW	980:983	arg1	diet					985:988	CHOW diet	980:988	CHOW diet groups	980:995	Relative to CHOW diet groups, the HFHS diet was associated with impaired force production in (a) MHC I fibres in the ADULT24 cohort; and (b) MHC IIa fibres in the ADULT12 and ADULT24 cohorts combined.
34139131	3	13	dep	adults	751:756	arg1	duration					767:774	12-week duration	759:774	12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort	759:824	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	6	14	theme	velocity	1283:1290	arg1	Ca2+-sensitivity					1225:1240	the Ca2+-sensitivity	1221:1240	the Ca2+-sensitivity of force production, unloaded shortening velocity, or ratio of active force to active stiffness in any cohort	1221:1350	However, the HFHS diet did not significantly affect the Ca2+-sensitivity of force production, unloaded shortening velocity, or ratio of active force to active stiffness in any cohort.
34139131	4	15	theme	HFHS-fed	828:835	arg1	rats					837:840	HFHS-fed rats	828:840	HFHS-fed rats	828:840	HFHS-fed rats had higher (P < 0.05) whole-body adiposity (derived from dual-energy X-ray absorptiometry) than CHOW-fed rats in all cohorts.
34139131	3	16	theme	high	593:596	arg1	beginning					644:652	a standard (CHOW) diet beginning	621:652	a standard (CHOW) diet beginning	621:652	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	3	16	theme	high	593:596	arg1	diet					613:616	a high-fat, high sucrose (HFHS) diet	581:616	diet	613:616	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	3	16	theme	high	593:596	arg1	weanlings					664:672	either weanlings	657:672	either weanlings	657:672	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	3	17	theme	24-week	793:799	arg1	cohort					785:790	ADULT12 cohort	777:790	ADULT12 cohort	777:790	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	3	17	theme	24-week	793:799	arg1	duration					801:808	24-week duration	793:808	24-week duration	793:808	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	7	18	theme	adult	1449:1453	arg1	rats					1455:1458	adult rats	1449:1458	adult rats	1449:1458	We conclude that diet-induced obesity can impair force output of permeabilized muscle fibres of adult rats.
34139131	3	19	theme	adults	751:756	arg1	function					409:416	contractile function	397:416	contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort)	397:825	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	1	20	theme	health	142:147	arg1	concern					149:155	a worldwide health concern	130:155	a worldwide health concern associated with impaired physical function	130:198	Obesity is a worldwide health concern associated with impaired physical function.
34139131	1	20	theme	health	142:147	arg1	Obesity					119:125	Obesity	119:125	Obesity	119:125	Obesity is a worldwide health concern associated with impaired physical function.
34139131	7	21	theme	fibres	1439:1444	arg1	output					1408:1413	force output	1402:1413	force output of permeabilized muscle fibres of adult rats	1402:1458	We conclude that diet-induced obesity can impair force output of permeabilized muscle fibres of adult rats.
34139131	3	22	theme	study	344:348	arg1	purpose					328:334	The purpose	324:334	The purpose of this study	324:348	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	8	23	theme	fibres	1536:1541	arg1	function					1494:1501	contractile function	1482:1501	contractile function of permeabilized skeletal muscle fibres	1482:1541	Novelty: We assessed contractile function of permeabilized skeletal muscle fibres in a rat model of diet-induced obesity.
34139131	6	24	theme	shortening	1272:1281	arg1	velocity					1283:1290	unloaded shortening velocity	1263:1290	unloaded shortening velocity	1263:1290	However, the HFHS diet did not significantly affect the Ca2+-sensitivity of force production, unloaded shortening velocity, or ratio of active force to active stiffness in any cohort.
34139131	7	25	theme	permeabilized	1418:1430	arg1	fibres					1439:1444	permeabilized muscle fibres	1418:1444	permeabilized muscle fibres of adult rats	1418:1458	We conclude that diet-induced obesity can impair force output of permeabilized muscle fibres of adult rats.
34139131	4	26	theme	dual-energy	899:909	arg1	absorptiometry					917:930	dual-energy X-ray absorptiometry	899:930	dual-energy X-ray absorptiometry	899:930	HFHS-fed rats had higher (P < 0.05) whole-body adiposity (derived from dual-energy X-ray absorptiometry) than CHOW-fed rats in all cohorts.
34139131	5	27	dep	fibres	1071:1076	arg1	a					1062:1062	a	1062:1062	a	1062:1062	Relative to CHOW diet groups, the HFHS diet was associated with impaired force production in (a) MHC I fibres in the ADULT24 cohort; and (b) MHC IIa fibres in the ADULT12 and ADULT24 cohorts combined.
34139131	5	28	theme	MHC	1109:1111	arg1	fibres					1117:1122	MHC IIa fibres	1109:1122	(b) MHC IIa fibres in the ADULT12 and ADULT24 cohorts combined	1105:1166	Relative to CHOW diet groups, the HFHS diet was associated with impaired force production in (a) MHC I fibres in the ADULT24 cohort; and (b) MHC IIa fibres in the ADULT12 and ADULT24 cohorts combined.
34139131	5	29	theme	impaired	1032:1039	arg1	production					1047:1056	impaired force production	1032:1056	impaired force production in (a) MHC I fibres in the ADULT24 cohort; and (b) MHC IIa fibres in the ADULT12 and ADULT24 cohorts combined	1032:1166	Relative to CHOW diet groups, the HFHS diet was associated with impaired force production in (a) MHC I fibres in the ADULT24 cohort; and (b) MHC IIa fibres in the ADULT12 and ADULT24 cohorts combined.
34139131	9	30	theme	MHC	1678:1680	arg1	I					1682:1682	MHC I	1678:1682	MHC I	1678:1682	The high-fat, high-sucrose diet was associated with impaired force output of fibres expressing MHC I or MHC IIa in some cohorts of rats.
34139131	1	31	theme	impaired	173:180	arg1	function					191:198	impaired physical function	173:198	impaired physical function	173:198	Obesity is a worldwide health concern associated with impaired physical function.
34139131	3	32	theme	12-week	759:765	arg1	duration					767:774	12-week duration	759:774	12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort	759:824	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	0	33	theme	diet	101:104	arg1	consumption					106:116	diet consumption	101:116	diet consumption	101:116	Contractility of permeabilized rat vastus intermedius muscle fibres following high-fat, high-sucrose diet consumption.
34139131	4	34	dep	higher	846:851	arg1	<					856:856	P < 0.05	854:861	P < 0.05	854:861	HFHS-fed rats had higher (P < 0.05) whole-body adiposity (derived from dual-energy X-ray absorptiometry) than CHOW-fed rats in all cohorts.
34139131	6	35	theme	unloaded	1263:1270	arg1	velocity					1283:1290	unloaded shortening velocity	1263:1290	unloaded shortening velocity	1263:1290	However, the HFHS diet did not significantly affect the Ca2+-sensitivity of force production, unloaded shortening velocity, or ratio of active force to active stiffness in any cohort.
34139131	3	36	theme	contractile	397:407	arg1	function					409:416	contractile function	397:416	contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort)	397:825	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	9	37	theme	high-fat	1587:1594	arg1	diet					1610:1613	The high-fat, high-sucrose diet	1583:1613	diet	1610:1613	The high-fat, high-sucrose diet was associated with impaired force output of fibres expressing MHC I or MHC IIa in some cohorts of rats.
34139131	3	38	theme	intermedius	453:463	arg1	fibres					465:470	chemically permeabilized vastus intermedius fibres	421:470	chemically permeabilized vastus intermedius fibres	421:470	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	0	39	theme	permeabilized	17:29	arg1	fibres					61:66	permeabilized rat vastus intermedius muscle fibres	17:66	permeabilized rat vastus intermedius muscle fibres	17:66	Contractility of permeabilized rat vastus intermedius muscle fibres following high-fat, high-sucrose diet consumption.
34139131	5	40	theme	I	1069:1069	arg1	fibres					1071:1076	MHC I fibres	1065:1076	(a) MHC I fibres in the ADULT24 cohort	1061:1098	Relative to CHOW diet groups, the HFHS diet was associated with impaired force production in (a) MHC I fibres in the ADULT24 cohort; and (b) MHC IIa fibres in the ADULT12 and ADULT24 cohorts combined.
34139131	3	41	theme	slow	548:551	arg1	Rats					560:563	slow MHC I. Rats	548:563	slow MHC I. Rats	548:563	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	7	42	theme	diet-induced	1370:1381	arg1	obesity					1383:1389	diet-induced obesity	1370:1389	diet-induced obesity	1370:1389	We conclude that diet-induced obesity can impair force output of permeabilized muscle fibres of adult rats.
34139131	3	43	theme	permeabilized	432:444	arg1	fibres					465:470	chemically permeabilized vastus intermedius fibres	421:470	chemically permeabilized vastus intermedius fibres	421:470	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	3	44	theme	CHOW	633:636	arg1	diet					639:642	a standard (CHOW) diet	621:642	a standard (CHOW) diet beginning	621:652	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	3	45	theme	Sprague-Dawley	480:493	arg1	rats					495:498	male Sprague-Dawley rats	475:498	male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa	475:543	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	3	46	dep	duration	682:689	arg1	duration					717:724	14-week duration	709:724	14-week duration	709:724	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	3	46	dep	duration	682:689	arg1	cohort					734:739	WEAN14 cohort	727:739	7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort	675:739	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	3	46	dep	duration	682:689	arg1	cohort					698:703	WEAN7 cohort	692:703	WEAN7 cohort	692:703	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	4	47	theme	X-ray	911:915	arg1	absorptiometry					917:930	dual-energy X-ray absorptiometry	899:930	dual-energy X-ray absorptiometry	899:930	HFHS-fed rats had higher (P < 0.05) whole-body adiposity (derived from dual-energy X-ray absorptiometry) than CHOW-fed rats in all cohorts.
34139131	3	48	theme	standard	623:630	arg1	diet					639:642	a standard (CHOW) diet	621:642	a standard (CHOW) diet beginning	621:652	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	3	49	theme	chain	529:533	arg1	IIa					541:543	fast myosin heavy chain (MHC) IIa	511:543	fast myosin heavy chain (MHC) IIa	511:543	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	0	50	theme	muscle	54:59	arg1	fibres					61:66	permeabilized rat vastus intermedius muscle fibres	17:66	permeabilized rat vastus intermedius muscle fibres	17:66	Contractility of permeabilized rat vastus intermedius muscle fibres following high-fat, high-sucrose diet consumption.
34139131	6	51	from	stiffness	1328:1336	arg1	cohort					1345:1350	any cohort	1341:1350	any cohort	1341:1350	However, the HFHS diet did not significantly affect the Ca2+-sensitivity of force production, unloaded shortening velocity, or ratio of active force to active stiffness in any cohort.
34139131	9	52	theme	MHC	1687:1689	arg1	IIa					1691:1693	MHC IIa	1687:1693	MHC IIa	1687:1693	The high-fat, high-sucrose diet was associated with impaired force output of fibres expressing MHC I or MHC IIa in some cohorts of rats.
34139131	3	53	theme	myosin	516:521	arg1	IIa					541:543	fast myosin heavy chain (MHC) IIa	511:543	fast myosin heavy chain (MHC) IIa	511:543	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	8	54	theme	contractile	1482:1492	arg1	function					1494:1501	contractile function	1482:1501	contractile function of permeabilized skeletal muscle fibres	1482:1541	Novelty: We assessed contractile function of permeabilized skeletal muscle fibres in a rat model of diet-induced obesity.
34139131	3	55	theme	MHC	553:555	arg1	Rats					560:563	slow MHC I. Rats	548:563	slow MHC I. Rats	548:563	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	3	56	dep	rats	495:498	arg1	duration					682:689	7-week duration	675:689	7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort	675:739	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	3	57	theme	diet	639:642	arg1	diet					613:616	a high-fat, high sucrose (HFHS) diet	581:616	diet	613:616	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	3	57	theme	diet	639:642	arg1	beginning					644:652	a standard (CHOW) diet beginning	621:652	a standard (CHOW) diet beginning	621:652	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	3	57	theme	diet	639:642	arg1	weanlings					664:672	either weanlings	657:672	either weanlings	657:672	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	6	58	theme	production	1251:1260	arg1	Ca2+-sensitivity					1225:1240	the Ca2+-sensitivity	1221:1240	the Ca2+-sensitivity of force production, unloaded shortening velocity, or ratio of active force to active stiffness in any cohort	1221:1350	However, the HFHS diet did not significantly affect the Ca2+-sensitivity of force production, unloaded shortening velocity, or ratio of active force to active stiffness in any cohort.
34139131	3	59	theme	7-week	675:680	arg1	duration					682:689	7-week duration	675:689	7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort	675:739	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	3	60	theme	MHC	536:538	arg1	IIa					541:543	fast myosin heavy chain (MHC) IIa	511:543	fast myosin heavy chain (MHC) IIa	511:543	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	6	61	theme	active	1305:1310	arg1	force					1312:1316	active force	1305:1316	active force	1305:1316	However, the HFHS diet did not significantly affect the Ca2+-sensitivity of force production, unloaded shortening velocity, or ratio of active force to active stiffness in any cohort.
34139131	4	62	from	adiposity	875:883	arg1	cohorts					959:965	all cohorts	955:965	all cohorts	955:965	HFHS-fed rats had higher (P < 0.05) whole-body adiposity (derived from dual-energy X-ray absorptiometry) than CHOW-fed rats in all cohorts.
34139131	9	63	theme	rats	1714:1717	arg1	cohorts					1703:1709	some cohorts	1698:1709	some cohorts of rats	1698:1717	The high-fat, high-sucrose diet was associated with impaired force output of fibres expressing MHC I or MHC IIa in some cohorts of rats.
34139131	10	64	theme	Other	1720:1724	arg1	measures					1726:1733	Other measures	1720:1733	Other measures of contractile function	1720:1757	Other measures of contractile function were not significantly affected by diet.
34139131	5	65	from	production	1047:1056	arg1	fibres					1117:1122	MHC IIa fibres	1109:1122	(b) MHC IIa fibres in the ADULT12 and ADULT24 cohorts combined	1105:1166	Relative to CHOW diet groups, the HFHS diet was associated with impaired force production in (a) MHC I fibres in the ADULT24 cohort; and (b) MHC IIa fibres in the ADULT12 and ADULT24 cohorts combined.
34139131	5	65	from	production	1047:1056	arg1	fibres					1071:1076	MHC I fibres	1065:1076	(a) MHC I fibres in the ADULT24 cohort	1061:1098	Relative to CHOW diet groups, the HFHS diet was associated with impaired force production in (a) MHC I fibres in the ADULT24 cohort; and (b) MHC IIa fibres in the ADULT12 and ADULT24 cohorts combined.
34139131	3	66	theme	I.	557:558	arg1	Rats					560:563	slow MHC I. Rats	548:563	slow MHC I. Rats	548:563	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	6	67	theme	ratio	1296:1300	arg1	Ca2+-sensitivity					1225:1240	the Ca2+-sensitivity	1221:1240	the Ca2+-sensitivity of force production, unloaded shortening velocity, or ratio of active force to active stiffness in any cohort	1221:1350	However, the HFHS diet did not significantly affect the Ca2+-sensitivity of force production, unloaded shortening velocity, or ratio of active force to active stiffness in any cohort.
34139131	5	68	theme	ADULT24	1085:1091	arg1	cohort					1093:1098	the ADULT24 cohort	1081:1098	the ADULT24 cohort	1081:1098	Relative to CHOW diet groups, the HFHS diet was associated with impaired force production in (a) MHC I fibres in the ADULT24 cohort; and (b) MHC IIa fibres in the ADULT12 and ADULT24 cohorts combined.
34139131	5	69	from	fibres	1117:1122	arg1	cohort					1093:1098	the ADULT24 cohort	1081:1098	the ADULT24 cohort	1081:1098	Relative to CHOW diet groups, the HFHS diet was associated with impaired force production in (a) MHC I fibres in the ADULT24 cohort; and (b) MHC IIa fibres in the ADULT12 and ADULT24 cohorts combined.
34139131	5	69	from	fibres	1117:1122	arg1	cohorts					1151:1157	the ADULT12 and ADULT24 cohorts	1127:1157	cohorts	1151:1157	Relative to CHOW diet groups, the HFHS diet was associated with impaired force production in (a) MHC I fibres in the ADULT24 cohort; and (b) MHC IIa fibres in the ADULT12 and ADULT24 cohorts combined.
34139131	2	70	theme	function	292:299	arg1	impairment					271:280	the impairment	267:280	the impairment of muscle function observed with obesity	267:321	It is not clear if contractile protein dysfunction contributes to the impairment of muscle function observed with obesity.
34139131	1	71	theme	physical	182:189	arg1	function					191:198	impaired physical function	173:198	impaired physical function	173:198	Obesity is a worldwide health concern associated with impaired physical function.
34139131	6	72	theme	force	1245:1249	arg1	production					1251:1260	force production	1245:1260	force production	1245:1260	However, the HFHS diet did not significantly affect the Ca2+-sensitivity of force production, unloaded shortening velocity, or ratio of active force to active stiffness in any cohort.
34139131	10	73	theme	function	1750:1757	arg1	measures					1726:1733	Other measures	1720:1733	Other measures of contractile function	1720:1757	Other measures of contractile function were not significantly affected by diet.
34139131	6	74	theme	HFHS	1182:1185	arg1	diet					1187:1190	the HFHS diet	1178:1190	the HFHS diet	1178:1190	However, the HFHS diet did not significantly affect the Ca2+-sensitivity of force production, unloaded shortening velocity, or ratio of active force to active stiffness in any cohort.
34139131	8	75	theme	diet-induced	1561:1572	arg1	obesity					1574:1580	diet-induced obesity	1561:1580	diet-induced obesity	1561:1580	Novelty: We assessed contractile function of permeabilized skeletal muscle fibres in a rat model of diet-induced obesity.
34139131	4	76	theme	higher	846:851	arg1	adiposity					875:883	higher (P < 0.05) whole-body adiposity	846:883	higher (P < 0.05) whole-body adiposity (derived from dual-energy X-ray absorptiometry) than CHOW-fed rats in all cohorts	846:965	HFHS-fed rats had higher (P < 0.05) whole-body adiposity (derived from dual-energy X-ray absorptiometry) than CHOW-fed rats in all cohorts.
34139131	5	77	theme	diet	985:988	arg1	groups					990:995	CHOW diet groups	980:995	CHOW diet groups	980:995	Relative to CHOW diet groups, the HFHS diet was associated with impaired force production in (a) MHC I fibres in the ADULT24 cohort; and (b) MHC IIa fibres in the ADULT12 and ADULT24 cohorts combined.
34139131	3	78	theme	sucrose	598:604	arg1	beginning					644:652	a standard (CHOW) diet beginning	621:652	a standard (CHOW) diet beginning	621:652	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	3	78	theme	sucrose	598:604	arg1	diet					613:616	a high-fat, high sucrose (HFHS) diet	581:616	diet	613:616	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	3	78	theme	sucrose	598:604	arg1	weanlings					664:672	either weanlings	657:672	either weanlings	657:672	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	7	79	theme	rats	1455:1458	arg1	fibres					1439:1444	permeabilized muscle fibres	1418:1444	permeabilized muscle fibres of adult rats	1418:1458	We conclude that diet-induced obesity can impair force output of permeabilized muscle fibres of adult rats.
34139131	4	80	theme	P	854:854	arg1	<					856:856	P < 0.05	854:861	P < 0.05	854:861	HFHS-fed rats had higher (P < 0.05) whole-body adiposity (derived from dual-energy X-ray absorptiometry) than CHOW-fed rats in all cohorts.
34139131	2	81	theme	protein	232:238	arg1	dysfunction					240:250	contractile protein dysfunction	220:250	contractile protein dysfunction	220:250	It is not clear if contractile protein dysfunction contributes to the impairment of muscle function observed with obesity.
34139131	3	82	theme	WEAN14	727:732	arg1	cohort					734:739	WEAN14 cohort	727:739	7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort	675:739	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	3	83	dep	duration	767:774	arg1	cohort					785:790	ADULT12 cohort	777:790	ADULT12 cohort	777:790	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	3	83	dep	duration	767:774	arg1	duration					801:808	24-week duration	793:808	24-week duration	793:808	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	3	83	dep	duration	767:774	arg1	cohort					819:824	ADULT24 cohort	811:824	12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort	759:824	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	1	84	theme	worldwide	132:140	arg1	concern					149:155	a worldwide health concern	130:155	a worldwide health concern associated with impaired physical function	130:198	Obesity is a worldwide health concern associated with impaired physical function.
34139131	1	84	theme	worldwide	132:140	arg1	Obesity					119:125	Obesity	119:125	Obesity	119:125	Obesity is a worldwide health concern associated with impaired physical function.
34139131	7	85	theme	muscle	1432:1437	arg1	fibres					1439:1444	permeabilized muscle fibres	1418:1444	permeabilized muscle fibres of adult rats	1418:1458	We conclude that diet-induced obesity can impair force output of permeabilized muscle fibres of adult rats.
34139131	5	86	theme	ADULT12	1131:1137	arg1	cohorts					1151:1157	the ADULT12 and ADULT24 cohorts	1127:1157	cohorts	1151:1157	Relative to CHOW diet groups, the HFHS diet was associated with impaired force production in (a) MHC I fibres in the ADULT24 cohort; and (b) MHC IIa fibres in the ADULT12 and ADULT24 cohorts combined.
34139131	3	87	theme	HFHS	607:610	arg1	beginning					644:652	a standard (CHOW) diet beginning	621:652	a standard (CHOW) diet beginning	621:652	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	3	87	theme	HFHS	607:610	arg1	diet					613:616	a high-fat, high sucrose (HFHS) diet	581:616	diet	613:616	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	3	87	theme	HFHS	607:610	arg1	weanlings					664:672	either weanlings	657:672	either weanlings	657:672	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	8	88	theme	permeabilized	1506:1518	arg1	fibres					1536:1541	permeabilized skeletal muscle fibres	1506:1541	permeabilized skeletal muscle fibres	1506:1541	Novelty: We assessed contractile function of permeabilized skeletal muscle fibres in a rat model of diet-induced obesity.
34139131	3	89	theme	young	745:749	arg1	adults					751:756	young adults	745:756	young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort)	745:825	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	7	90	theme	force	1402:1406	arg1	output					1408:1413	force output	1402:1413	force output of permeabilized muscle fibres of adult rats	1402:1458	We conclude that diet-induced obesity can impair force output of permeabilized muscle fibres of adult rats.
34139131	5	91	theme	IIa	1113:1115	arg1	fibres					1117:1122	MHC IIa fibres	1109:1122	(b) MHC IIa fibres in the ADULT12 and ADULT24 cohorts combined	1105:1166	Relative to CHOW diet groups, the HFHS diet was associated with impaired force production in (a) MHC I fibres in the ADULT24 cohort; and (b) MHC IIa fibres in the ADULT12 and ADULT24 cohorts combined.
34139131	5	92	theme	force	1041:1045	arg1	production					1047:1056	impaired force production	1032:1056	impaired force production in (a) MHC I fibres in the ADULT24 cohort; and (b) MHC IIa fibres in the ADULT12 and ADULT24 cohorts combined	1032:1166	Relative to CHOW diet groups, the HFHS diet was associated with impaired force production in (a) MHC I fibres in the ADULT24 cohort; and (b) MHC IIa fibres in the ADULT12 and ADULT24 cohorts combined.
34139131	3	93	theme	diet-induced	368:379	arg1	obesity					381:387	diet-induced obesity	368:387	diet-induced obesity	368:387	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	5	94	theme	HFHS	1002:1005	arg1	diet					1007:1010	the HFHS diet	998:1010	the HFHS diet	998:1010	Relative to CHOW diet groups, the HFHS diet was associated with impaired force production in (a) MHC I fibres in the ADULT24 cohort; and (b) MHC IIa fibres in the ADULT12 and ADULT24 cohorts combined.
34139131	4	95	contain	had	842:844	arg1	rats					837:840	HFHS-fed rats	828:840	HFHS-fed rats	828:840	HFHS-fed rats had higher (P < 0.05) whole-body adiposity (derived from dual-energy X-ray absorptiometry) than CHOW-fed rats in all cohorts.
34139131	4	95	contain	had	842:844	arg2	adiposity					875:883	higher (P < 0.05) whole-body adiposity	846:883	higher (P < 0.05) whole-body adiposity (derived from dual-energy X-ray absorptiometry) than CHOW-fed rats in all cohorts	846:965	HFHS-fed rats had higher (P < 0.05) whole-body adiposity (derived from dual-energy X-ray absorptiometry) than CHOW-fed rats in all cohorts.
34139131	4	96	theme	whole-body	864:873	arg1	adiposity					875:883	higher (P < 0.05) whole-body adiposity	846:883	higher (P < 0.05) whole-body adiposity (derived from dual-energy X-ray absorptiometry) than CHOW-fed rats in all cohorts	846:965	HFHS-fed rats had higher (P < 0.05) whole-body adiposity (derived from dual-energy X-ray absorptiometry) than CHOW-fed rats in all cohorts.
34139131	3	97	theme	male	475:478	arg1	rats					495:498	male Sprague-Dawley rats	475:498	male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa	475:543	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	4	98	theme	CHOW-fed	938:945	arg1	rats					947:950	CHOW-fed rats	938:950	CHOW-fed rats	938:950	HFHS-fed rats had higher (P < 0.05) whole-body adiposity (derived from dual-energy X-ray absorptiometry) than CHOW-fed rats in all cohorts.
34139131	5	99	theme	ADULT24	1143:1149	arg1	cohorts					1151:1157	the ADULT12 and ADULT24 cohorts	1127:1157	cohorts	1151:1157	Relative to CHOW diet groups, the HFHS diet was associated with impaired force production in (a) MHC I fibres in the ADULT24 cohort; and (b) MHC IIa fibres in the ADULT12 and ADULT24 cohorts combined.
34139131	3	100	theme	fibres	465:470	arg1	function					409:416	contractile function	397:416	contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort)	397:825	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	0	101	theme	rat	31:33	arg1	fibres					61:66	permeabilized rat vastus intermedius muscle fibres	17:66	permeabilized rat vastus intermedius muscle fibres	17:66	Contractility of permeabilized rat vastus intermedius muscle fibres following high-fat, high-sucrose diet consumption.
34139131	5	102	theme	MHC	1065:1067	arg1	fibres					1071:1076	MHC I fibres	1065:1076	(a) MHC I fibres in the ADULT24 cohort	1061:1098	Relative to CHOW diet groups, the HFHS diet was associated with impaired force production in (a) MHC I fibres in the ADULT24 cohort; and (b) MHC IIa fibres in the ADULT12 and ADULT24 cohorts combined.
34139131	0	103	dep	high-fat	78:85	arg1	consumption					106:116	diet consumption	101:116	diet consumption	101:116	Contractility of permeabilized rat vastus intermedius muscle fibres following high-fat, high-sucrose diet consumption.
34139131	9	104	theme	fibres	1660:1665	arg1	output					1650:1655	impaired force output	1635:1655	impaired force output of fibres expressing MHC I or MHC IIa in some cohorts of rats	1635:1717	The high-fat, high-sucrose diet was associated with impaired force output of fibres expressing MHC I or MHC IIa in some cohorts of rats.
34139131	3	105	theme	fast	511:514	arg1	IIa					541:543	fast myosin heavy chain (MHC) IIa	511:543	fast myosin heavy chain (MHC) IIa	511:543	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	0	106	theme	intermedius	42:52	arg1	fibres					61:66	permeabilized rat vastus intermedius muscle fibres	17:66	permeabilized rat vastus intermedius muscle fibres	17:66	Contractility of permeabilized rat vastus intermedius muscle fibres following high-fat, high-sucrose diet consumption.
34139131	3	107	theme	vastus	446:451	arg1	fibres					465:470	chemically permeabilized vastus intermedius fibres	421:470	chemically permeabilized vastus intermedius fibres	421:470	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	3	108	theme	rats	495:498	arg1	function					409:416	contractile function	397:416	contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort)	397:825	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	0	109	theme	fibres	61:66	arg1	Contractility					0:12	Contractility	0:12	Contractility of permeabilized rat vastus intermedius muscle fibres	0:66	Contractility of permeabilized rat vastus intermedius muscle fibres following high-fat, high-sucrose diet consumption.
34139131	4	110	attach	derived	886:892	arg2	adiposity					875:883	higher (P < 0.05) whole-body adiposity	846:883	higher (P < 0.05) whole-body adiposity (derived from dual-energy X-ray absorptiometry) than CHOW-fed rats in all cohorts	846:965	HFHS-fed rats had higher (P < 0.05) whole-body adiposity (derived from dual-energy X-ray absorptiometry) than CHOW-fed rats in all cohorts.
34139131	4	110	attach	derived	886:892	arg1	absorptiometry					917:930	dual-energy X-ray absorptiometry	899:930	dual-energy X-ray absorptiometry	899:930	HFHS-fed rats had higher (P < 0.05) whole-body adiposity (derived from dual-energy X-ray absorptiometry) than CHOW-fed rats in all cohorts.
34139131	9	111	theme	impaired	1635:1642	arg1	output					1650:1655	impaired force output	1635:1655	impaired force output of fibres expressing MHC I or MHC IIa in some cohorts of rats	1635:1717	The high-fat, high-sucrose diet was associated with impaired force output of fibres expressing MHC I or MHC IIa in some cohorts of rats.
34139131	3	112	theme	heavy	523:527	arg1	IIa					541:543	fast myosin heavy chain (MHC) IIa	511:543	fast myosin heavy chain (MHC) IIa	511:543	The purpose of this study was to examine if diet-induced obesity affects contractile function of chemically permeabilized vastus intermedius fibres of male Sprague-Dawley rats expressing fast myosin heavy chain (MHC) IIa or slow MHC I. Rats consumed either a high-fat, high sucrose (HFHS) diet or a standard (CHOW) diet beginning as either weanlings (7-week duration: WEAN7 cohort, or 14-week duration: WEAN14 cohort) or young adults (12-week duration: ADULT12 cohort, 24-week duration: ADULT24 cohort).
34139131	6	113	theme	active	1321:1326	arg1	stiffness					1328:1336	active stiffness	1321:1336	active stiffness in any cohort	1321:1350	However, the HFHS diet did not significantly affect the Ca2+-sensitivity of force production, unloaded shortening velocity, or ratio of active force to active stiffness in any cohort.
34139131	8	114	theme	muscle	1529:1534	arg1	fibres					1536:1541	permeabilized skeletal muscle fibres	1506:1541	permeabilized skeletal muscle fibres	1506:1541	Novelty: We assessed contractile function of permeabilized skeletal muscle fibres in a rat model of diet-induced obesity.
34139131	10	115	theme	contractile	1738:1748	arg1	function					1750:1757	contractile function	1738:1757	contractile function	1738:1757	Other measures of contractile function were not significantly affected by diet.
34139131	9	116	theme	force	1644:1648	arg1	output					1650:1655	impaired force output	1635:1655	impaired force output of fibres expressing MHC I or MHC IIa in some cohorts of rats	1635:1717	The high-fat, high-sucrose diet was associated with impaired force output of fibres expressing MHC I or MHC IIa in some cohorts of rats.
34139131	6	117	theme	force	1312:1316	arg1	velocity					1283:1290	unloaded shortening velocity	1263:1290	unloaded shortening velocity	1263:1290	However, the HFHS diet did not significantly affect the Ca2+-sensitivity of force production, unloaded shortening velocity, or ratio of active force to active stiffness in any cohort.
34139131	6	117	theme	force	1312:1316	arg1	ratio					1296:1300	ratio	1296:1300	ratio of active force to active stiffness in any cohort	1296:1350	However, the HFHS diet did not significantly affect the Ca2+-sensitivity of force production, unloaded shortening velocity, or ratio of active force to active stiffness in any cohort.
34139131	6	117	theme	force	1312:1316	arg1	production					1251:1260	force production	1245:1260	force production	1245:1260	However, the HFHS diet did not significantly affect the Ca2+-sensitivity of force production, unloaded shortening velocity, or ratio of active force to active stiffness in any cohort.
34691703	4	0	theme	photosynthesis	1179:1192	arg1	system					1194:1199	a successful semi-solid artificial photosynthesis system	1144:1199	a successful semi-solid artificial photosynthesis system for hydrogen evolution	1144:1222	Subsequently, using sugar cubes as templates, we demonstrate that 3D fabricated porous structures can become colonized by engineered bacteria and can be functionalized with highly photoreactive minerals, thereby enabling co-localization of the photocatalytic units with a bacteria-based hydrogenase reaction for a successful semi-solid artificial photosynthesis system for hydrogen evolution.
34691703	5	1	theme	biofabrication	1330:1343	arg1	techniques					1345:1354	biofabrication techniques	1330:1354	biofabrication techniques	1330:1354	Our study thus highlights the power of coupling genetically engineered proteins and polysaccharides with biofabrication techniques to generate hierarchically organized mineralized porous structures inspired by nature.
34691703	4	2	theme	sugar	852:856	arg1	templates					867:875	templates	867:875	templates	867:875	Subsequently, using sugar cubes as templates, we demonstrate that 3D fabricated porous structures can become colonized by engineered bacteria and can be functionalized with highly photoreactive minerals, thereby enabling co-localization of the photocatalytic units with a bacteria-based hydrogenase reaction for a successful semi-solid artificial photosynthesis system for hydrogen evolution.
34691703	4	2	theme	sugar	852:856	arg1	cubes					858:862	sugar cubes	852:862	sugar cubes	852:862	Subsequently, using sugar cubes as templates, we demonstrate that 3D fabricated porous structures can become colonized by engineered bacteria and can be functionalized with highly photoreactive minerals, thereby enabling co-localization of the photocatalytic units with a bacteria-based hydrogenase reaction for a successful semi-solid artificial photosynthesis system for hydrogen evolution.
34691703	4	3	theme	units	1091:1095	arg1	co-localization					1053:1067	co-localization	1053:1067	co-localization of the photocatalytic units with a bacteria-based hydrogenase reaction for a successful semi-solid artificial photosynthesis system for hydrogen evolution	1053:1222	Subsequently, using sugar cubes as templates, we demonstrate that 3D fabricated porous structures can become colonized by engineered bacteria and can be functionalized with highly photoreactive minerals, thereby enabling co-localization of the photocatalytic units with a bacteria-based hydrogenase reaction for a successful semi-solid artificial photosynthesis system for hydrogen evolution.
34691703	2	4	theme	ordered	446:452	arg1	composites					454:463	hierarchically ordered composites	431:463	hierarchically ordered composites	431:463	Recapitulating the remarkable architectures and functions of diatom frustules in artificial materials is a major challenge that has important technological implications for hierarchically ordered composites.
34691703	3	5	theme	amyloid	638:644	arg1	proteins					653:660	genetically engineered amyloid fusion proteins	615:660	genetically engineered amyloid fusion proteins	615:660	Here, we report the construction of highly ordered, mineralized composites based on fabrication of complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3.
34691703	2	6	theme	major	365:369	arg1	challenge					371:379	a major challenge	363:379	a major challenge that has important technological implications for hierarchically ordered composites	363:463	Recapitulating the remarkable architectures and functions of diatom frustules in artificial materials is a major challenge that has important technological implications for hierarchically ordered composites.
34691703	5	7	theme	porous	1405:1410	arg1	structures					1412:1421	hierarchically organized mineralized porous structures	1368:1421	hierarchically organized mineralized porous structures inspired by nature	1368:1440	Our study thus highlights the power of coupling genetically engineered proteins and polysaccharides with biofabrication techniques to generate hierarchically organized mineralized porous structures inspired by nature.
34691703	4	8	theme	hydrogenase	1119:1129	arg1	reaction					1131:1138	a bacteria-based hydrogenase reaction	1102:1138	a bacteria-based hydrogenase reaction for a successful semi-solid artificial photosynthesis system for hydrogen evolution	1102:1222	Subsequently, using sugar cubes as templates, we demonstrate that 3D fabricated porous structures can become colonized by engineered bacteria and can be functionalized with highly photoreactive minerals, thereby enabling co-localization of the photocatalytic units with a bacteria-based hydrogenase reaction for a successful semi-solid artificial photosynthesis system for hydrogen evolution.
34691703	3	9	theme	natural	670:676	arg1	chitin-and					693:702	the natural polysaccharide chitin-and	666:702	the natural polysaccharide chitin-and	666:702	Here, we report the construction of highly ordered, mineralized composites based on fabrication of complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3.
34691703	1	10	theme	Marine	98:103	arg1	diatoms					105:111	Marine diatoms	98:111	Marine diatoms	98:111	Marine diatoms construct their hierarchically ordered, three-dimensional (3D) external structures called frustules through precise biomineralization processes.
34691703	3	11	theme	porous	589:594	arg1	structures-made					596:610	complex self-supporting porous structures-made	565:610	complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3	565:829	Here, we report the construction of highly ordered, mineralized composites based on fabrication of complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3.
34691703	5	12	theme	polysaccharides	1309:1323	arg1	power					1255:1259	the power	1251:1259	the power of coupling genetically engineered proteins and polysaccharides with biofabrication techniques to generate hierarchically organized mineralized porous structures inspired by nature	1251:1440	Our study thus highlights the power of coupling genetically engineered proteins and polysaccharides with biofabrication techniques to generate hierarchically organized mineralized porous structures inspired by nature.
34691703	2	13	dep	architectures	288:300	arg1	the					273:275	the	273:275	the	273:275	Recapitulating the remarkable architectures and functions of diatom frustules in artificial materials is a major challenge that has important technological implications for hierarchically ordered composites.
34691703	1	14	dep	three-dimensional	153:169	arg1	3D					172:173	3D	172:173	3D	172:173	Marine diatoms construct their hierarchically ordered, three-dimensional (3D) external structures called frustules through precise biomineralization processes.
34691703	2	15	from	architectures	288:300	arg1	materials					350:358	artificial materials	339:358	artificial materials	339:358	Recapitulating the remarkable architectures and functions of diatom frustules in artificial materials is a major challenge that has important technological implications for hierarchically ordered composites.
34691703	3	16	theme	composites	530:539	arg1	construction					486:497	the construction	482:497	the construction of highly ordered, mineralized composites based on fabrication of complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3	482:829	Here, we report the construction of highly ordered, mineralized composites based on fabrication of complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3.
34691703	4	17	theme	hydrogen	1205:1212	arg1	evolution					1214:1222	hydrogen evolution	1205:1222	hydrogen evolution	1205:1222	Subsequently, using sugar cubes as templates, we demonstrate that 3D fabricated porous structures can become colonized by engineered bacteria and can be functionalized with highly photoreactive minerals, thereby enabling co-localization of the photocatalytic units with a bacteria-based hydrogenase reaction for a successful semi-solid artificial photosynthesis system for hydrogen evolution.
34691703	1	18	theme	precise	221:227	arg1	processes					247:255	precise biomineralization processes	221:255	precise biomineralization processes	221:255	Marine diatoms construct their hierarchically ordered, three-dimensional (3D) external structures called frustules through precise biomineralization processes.
34691703	4	19	theme	artificial	1168:1177	arg1	system					1194:1199	a successful semi-solid artificial photosynthesis system	1144:1199	a successful semi-solid artificial photosynthesis system for hydrogen evolution	1144:1222	Subsequently, using sugar cubes as templates, we demonstrate that 3D fabricated porous structures can become colonized by engineered bacteria and can be functionalized with highly photoreactive minerals, thereby enabling co-localization of the photocatalytic units with a bacteria-based hydrogenase reaction for a successful semi-solid artificial photosynthesis system for hydrogen evolution.
34691703	4	20	theme	photoreactive	1012:1024	arg1	minerals					1026:1033	highly photoreactive minerals	1005:1033	highly photoreactive minerals	1005:1033	Subsequently, using sugar cubes as templates, we demonstrate that 3D fabricated porous structures can become colonized by engineered bacteria and can be functionalized with highly photoreactive minerals, thereby enabling co-localization of the photocatalytic units with a bacteria-based hydrogenase reaction for a successful semi-solid artificial photosynthesis system for hydrogen evolution.
34691703	2	21	theme	important	390:398	arg1	implications					414:425	important technological implications	390:425	important technological implications for hierarchically ordered composites	390:463	Recapitulating the remarkable architectures and functions of diatom frustules in artificial materials is a major challenge that has important technological implications for hierarchically ordered composites.
34691703	2	22	from	functions	306:314	arg1	materials					350:358	artificial materials	339:358	artificial materials	339:358	Recapitulating the remarkable architectures and functions of diatom frustules in artificial materials is a major challenge that has important technological implications for hierarchically ordered composites.
34691703	1	23	theme	biomineralization	229:245	arg1	processes					247:255	precise biomineralization processes	221:255	precise biomineralization processes	221:255	Marine diatoms construct their hierarchically ordered, three-dimensional (3D) external structures called frustules through precise biomineralization processes.
34691703	3	24	dep	ordered	509:515	arg1	mineralized					518:528	mineralized	518:528	mineralized	518:528	Here, we report the construction of highly ordered, mineralized composites based on fabrication of complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3.
34691703	3	25	theme	in	715:716	arg1	mineralization					751:764	in situ multiscale protein-mediated mineralization	715:764	in situ multiscale protein-mediated mineralization	715:764	Here, we report the construction of highly ordered, mineralized composites based on fabrication of complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3.
34691703	3	26	theme	complex	565:571	arg1	structures-made					596:610	complex self-supporting porous structures-made	565:610	complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3	565:829	Here, we report the construction of highly ordered, mineralized composites based on fabrication of complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3.
34691703	4	27	theme	engineered	954:963	arg1	bacteria					965:972	engineered bacteria	954:972	engineered bacteria	954:972	Subsequently, using sugar cubes as templates, we demonstrate that 3D fabricated porous structures can become colonized by engineered bacteria and can be functionalized with highly photoreactive minerals, thereby enabling co-localization of the photocatalytic units with a bacteria-based hydrogenase reaction for a successful semi-solid artificial photosynthesis system for hydrogen evolution.
34691703	1	28	theme	ordered	144:150	arg1	structures					185:194	their hierarchically ordered, three-dimensional (3D) external structures	123:194	their hierarchically ordered, three-dimensional (3D) external structures called frustules through precise biomineralization processes	123:255	Marine diatoms construct their hierarchically ordered, three-dimensional (3D) external structures called frustules through precise biomineralization processes.
34691703	5	29	with	proteins	1296:1303	arg1	techniques					1345:1354	biofabrication techniques	1330:1354	biofabrication techniques	1330:1354	Our study thus highlights the power of coupling genetically engineered proteins and polysaccharides with biofabrication techniques to generate hierarchically organized mineralized porous structures inspired by nature.
34691703	3	30	theme	proteins	653:660	arg1	structures-made					596:610	complex self-supporting porous structures-made	565:610	complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3	565:829	Here, we report the construction of highly ordered, mineralized composites based on fabrication of complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3.
34691703	5	31	theme	coupling	1264:1271	arg1	proteins					1296:1303	coupling genetically engineered proteins	1264:1303	coupling genetically engineered proteins	1264:1303	Our study thus highlights the power of coupling genetically engineered proteins and polysaccharides with biofabrication techniques to generate hierarchically organized mineralized porous structures inspired by nature.
34691703	3	32	theme	chitin-and	693:702	arg1	structures-made					596:610	complex self-supporting porous structures-made	565:610	complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3	565:829	Here, we report the construction of highly ordered, mineralized composites based on fabrication of complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3.
34691703	3	33	dep	in	715:716	arg1	situ					718:721	situ	718:721	situ	718:721	Here, we report the construction of highly ordered, mineralized composites based on fabrication of complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3.
34691703	1	34	theme	external	176:183	arg1	structures					185:194	their hierarchically ordered, three-dimensional (3D) external structures	123:194	their hierarchically ordered, three-dimensional (3D) external structures called frustules through precise biomineralization processes	123:255	Marine diatoms construct their hierarchically ordered, three-dimensional (3D) external structures called frustules through precise biomineralization processes.
34691703	0	35	theme	protein-polysaccharide	55:76	arg1	structures					86:95	patterned protein-polysaccharide complex structures	45:95	patterned protein-polysaccharide complex structures	45:95	Diatom-inspired multiscale mineralization of patterned protein-polysaccharide complex structures.
34691703	4	36	theme	bacteria-based	1104:1117	arg1	reaction					1131:1138	a bacteria-based hydrogenase reaction	1102:1138	a bacteria-based hydrogenase reaction for a successful semi-solid artificial photosynthesis system for hydrogen evolution	1102:1222	Subsequently, using sugar cubes as templates, we demonstrate that 3D fabricated porous structures can become colonized by engineered bacteria and can be functionalized with highly photoreactive minerals, thereby enabling co-localization of the photocatalytic units with a bacteria-based hydrogenase reaction for a successful semi-solid artificial photosynthesis system for hydrogen evolution.
34691703	3	37	theme	protein-mediated	734:749	arg1	mineralization					751:764	in situ multiscale protein-mediated mineralization	715:764	in situ multiscale protein-mediated mineralization	715:764	Here, we report the construction of highly ordered, mineralized composites based on fabrication of complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3.
34691703	4	38	with	co-localization	1053:1067	arg1	reaction					1131:1138	a bacteria-based hydrogenase reaction	1102:1138	a bacteria-based hydrogenase reaction for a successful semi-solid artificial photosynthesis system for hydrogen evolution	1102:1222	Subsequently, using sugar cubes as templates, we demonstrate that 3D fabricated porous structures can become colonized by engineered bacteria and can be functionalized with highly photoreactive minerals, thereby enabling co-localization of the photocatalytic units with a bacteria-based hydrogenase reaction for a successful semi-solid artificial photosynthesis system for hydrogen evolution.
34691703	0	39	theme	patterned	45:53	arg1	structures					86:95	patterned protein-polysaccharide complex structures	45:95	patterned protein-polysaccharide complex structures	45:95	Diatom-inspired multiscale mineralization of patterned protein-polysaccharide complex structures.
34691703	5	40	theme	organized	1383:1391	arg1	structures					1412:1421	hierarchically organized mineralized porous structures	1368:1421	hierarchically organized mineralized porous structures inspired by nature	1368:1440	Our study thus highlights the power of coupling genetically engineered proteins and polysaccharides with biofabrication techniques to generate hierarchically organized mineralized porous structures inspired by nature.
34691703	3	41	theme	ordered	509:515	arg1	composites					530:539	highly ordered, mineralized composites	502:539	highly ordered, mineralized composites based on fabrication of complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3	502:829	Here, we report the construction of highly ordered, mineralized composites based on fabrication of complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3.
34691703	4	42	theme	fabricated	901:910	arg1	structures					919:928	3D fabricated porous structures	898:928	3D fabricated porous structures	898:928	Subsequently, using sugar cubes as templates, we demonstrate that 3D fabricated porous structures can become colonized by engineered bacteria and can be functionalized with highly photoreactive minerals, thereby enabling co-localization of the photocatalytic units with a bacteria-based hydrogenase reaction for a successful semi-solid artificial photosynthesis system for hydrogen evolution.
34691703	4	43	theme	3D	898:899	arg1	structures					919:928	3D fabricated porous structures	898:928	3D fabricated porous structures	898:928	Subsequently, using sugar cubes as templates, we demonstrate that 3D fabricated porous structures can become colonized by engineered bacteria and can be functionalized with highly photoreactive minerals, thereby enabling co-localization of the photocatalytic units with a bacteria-based hydrogenase reaction for a successful semi-solid artificial photosynthesis system for hydrogen evolution.
34691703	0	44	theme	structures	86:95	arg1	mineralization					27:40	mineralization	27:40	mineralization of patterned protein-polysaccharide complex structures	27:95	Diatom-inspired multiscale mineralization of patterned protein-polysaccharide complex structures.
34691703	5	45	theme	mineralized	1393:1403	arg1	structures					1412:1421	hierarchically organized mineralized porous structures	1368:1421	hierarchically organized mineralized porous structures inspired by nature	1368:1440	Our study thus highlights the power of coupling genetically engineered proteins and polysaccharides with biofabrication techniques to generate hierarchically organized mineralized porous structures inspired by nature.
34691703	5	46	theme	proteins	1296:1303	arg1	power					1255:1259	the power	1251:1259	the power of coupling genetically engineered proteins and polysaccharides with biofabrication techniques to generate hierarchically organized mineralized porous structures inspired by nature	1251:1440	Our study thus highlights the power of coupling genetically engineered proteins and polysaccharides with biofabrication techniques to generate hierarchically organized mineralized porous structures inspired by nature.
34691703	3	47	theme	structures-made	596:610	arg1	fabrication					550:560	fabrication	550:560	fabrication of complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3	550:829	Here, we report the construction of highly ordered, mineralized composites based on fabrication of complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3.
34691703	0	48	theme	complex	78:84	arg1	structures					86:95	patterned protein-polysaccharide complex structures	45:95	patterned protein-polysaccharide complex structures	45:95	Diatom-inspired multiscale mineralization of patterned protein-polysaccharide complex structures.
34691703	2	49	contain	has	386:388	arg2	implications					414:425	important technological implications	390:425	important technological implications for hierarchically ordered composites	390:463	Recapitulating the remarkable architectures and functions of diatom frustules in artificial materials is a major challenge that has important technological implications for hierarchically ordered composites.
34691703	2	49	contain	has	386:388	arg1	challenge					371:379	a major challenge	363:379	a major challenge that has important technological implications for hierarchically ordered composites	363:463	Recapitulating the remarkable architectures and functions of diatom frustules in artificial materials is a major challenge that has important technological implications for hierarchically ordered composites.
34691703	2	50	theme	remarkable	277:286	arg1	architectures					288:300	remarkable architectures	277:300	remarkable architectures	277:300	Recapitulating the remarkable architectures and functions of diatom frustules in artificial materials is a major challenge that has important technological implications for hierarchically ordered composites.
34691703	3	51	theme	multiscale	723:732	arg1	mineralization					751:764	in situ multiscale protein-mediated mineralization	715:764	in situ multiscale protein-mediated mineralization	715:764	Here, we report the construction of highly ordered, mineralized composites based on fabrication of complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3.
34691703	3	52	theme	engineered	627:636	arg1	proteins					653:660	genetically engineered amyloid fusion proteins	615:660	genetically engineered amyloid fusion proteins	615:660	Here, we report the construction of highly ordered, mineralized composites based on fabrication of complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3.
34691703	5	53	theme	engineered	1285:1294	arg1	proteins					1296:1303	coupling genetically engineered proteins	1264:1303	coupling genetically engineered proteins	1264:1303	Our study thus highlights the power of coupling genetically engineered proteins and polysaccharides with biofabrication techniques to generate hierarchically organized mineralized porous structures inspired by nature.
34691703	3	54	theme	diverse	771:777	arg1	SiO2					810:813	SiO2	810:813	SiO2	810:813	Here, we report the construction of highly ordered, mineralized composites based on fabrication of complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3.
34691703	3	54	theme	diverse	771:777	arg1	Ga2O3					825:829	Ga2O3	825:829	Ga2O3	825:829	Here, we report the construction of highly ordered, mineralized composites based on fabrication of complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3.
34691703	3	54	theme	diverse	771:777	arg1	TiO2					816:819	TiO2	816:819	TiO2	816:819	Here, we report the construction of highly ordered, mineralized composites based on fabrication of complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3.
34691703	3	54	theme	diverse	771:777	arg1	materials					789:797	diverse inorganic materials	771:797	diverse inorganic materials	771:797	Here, we report the construction of highly ordered, mineralized composites based on fabrication of complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3.
34691703	4	55	theme	photocatalytic	1076:1089	arg1	units					1091:1095	the photocatalytic units	1072:1095	the photocatalytic units	1072:1095	Subsequently, using sugar cubes as templates, we demonstrate that 3D fabricated porous structures can become colonized by engineered bacteria and can be functionalized with highly photoreactive minerals, thereby enabling co-localization of the photocatalytic units with a bacteria-based hydrogenase reaction for a successful semi-solid artificial photosynthesis system for hydrogen evolution.
34691703	3	56	theme	inorganic	779:787	arg1	SiO2					810:813	SiO2	810:813	SiO2	810:813	Here, we report the construction of highly ordered, mineralized composites based on fabrication of complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3.
34691703	3	56	theme	inorganic	779:787	arg1	Ga2O3					825:829	Ga2O3	825:829	Ga2O3	825:829	Here, we report the construction of highly ordered, mineralized composites based on fabrication of complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3.
34691703	3	56	theme	inorganic	779:787	arg1	TiO2					816:819	TiO2	816:819	TiO2	816:819	Here, we report the construction of highly ordered, mineralized composites based on fabrication of complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3.
34691703	3	56	theme	inorganic	779:787	arg1	materials					789:797	diverse inorganic materials	771:797	diverse inorganic materials	771:797	Here, we report the construction of highly ordered, mineralized composites based on fabrication of complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3.
34691703	2	57	theme	artificial	339:348	arg1	materials					350:358	artificial materials	339:358	artificial materials	339:358	Recapitulating the remarkable architectures and functions of diatom frustules in artificial materials is a major challenge that has important technological implications for hierarchically ordered composites.
34691703	3	58	theme	self-supporting	573:587	arg1	structures-made					596:610	complex self-supporting porous structures-made	565:610	complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3	565:829	Here, we report the construction of highly ordered, mineralized composites based on fabrication of complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3.
34691703	3	59	theme	polysaccharide	678:691	arg1	chitin-and					693:702	the natural polysaccharide chitin-and	666:702	the natural polysaccharide chitin-and	666:702	Here, we report the construction of highly ordered, mineralized composites based on fabrication of complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3.
34691703	4	60	theme	porous	912:917	arg1	structures					919:928	3D fabricated porous structures	898:928	3D fabricated porous structures	898:928	Subsequently, using sugar cubes as templates, we demonstrate that 3D fabricated porous structures can become colonized by engineered bacteria and can be functionalized with highly photoreactive minerals, thereby enabling co-localization of the photocatalytic units with a bacteria-based hydrogenase reaction for a successful semi-solid artificial photosynthesis system for hydrogen evolution.
34691703	2	61	theme	technological	400:412	arg1	implications					414:425	important technological implications	390:425	important technological implications for hierarchically ordered composites	390:463	Recapitulating the remarkable architectures and functions of diatom frustules in artificial materials is a major challenge that has important technological implications for hierarchically ordered composites.
34691703	4	62	theme	semi-solid	1157:1166	arg1	system					1194:1199	a successful semi-solid artificial photosynthesis system	1144:1199	a successful semi-solid artificial photosynthesis system for hydrogen evolution	1144:1222	Subsequently, using sugar cubes as templates, we demonstrate that 3D fabricated porous structures can become colonized by engineered bacteria and can be functionalized with highly photoreactive minerals, thereby enabling co-localization of the photocatalytic units with a bacteria-based hydrogenase reaction for a successful semi-solid artificial photosynthesis system for hydrogen evolution.
34691703	2	63	theme	frustules	326:334	arg1	functions					306:314	functions	306:314	functions	306:314	Recapitulating the remarkable architectures and functions of diatom frustules in artificial materials is a major challenge that has important technological implications for hierarchically ordered composites.
34691703	2	63	theme	frustules	326:334	arg1	architectures					288:300	remarkable architectures	277:300	remarkable architectures	277:300	Recapitulating the remarkable architectures and functions of diatom frustules in artificial materials is a major challenge that has important technological implications for hierarchically ordered composites.
34691703	1	64	dep	ordered	144:150	arg1	three-dimensional					153:169	three-dimensional	153:169	three-dimensional	153:169	Marine diatoms construct their hierarchically ordered, three-dimensional (3D) external structures called frustules through precise biomineralization processes.
34691703	5	65	with	polysaccharides	1309:1323	arg1	techniques					1345:1354	biofabrication techniques	1330:1354	biofabrication techniques	1330:1354	Our study thus highlights the power of coupling genetically engineered proteins and polysaccharides with biofabrication techniques to generate hierarchically organized mineralized porous structures inspired by nature.
34691703	4	66	theme	successful	1146:1155	arg1	system					1194:1199	a successful semi-solid artificial photosynthesis system	1144:1199	a successful semi-solid artificial photosynthesis system for hydrogen evolution	1144:1222	Subsequently, using sugar cubes as templates, we demonstrate that 3D fabricated porous structures can become colonized by engineered bacteria and can be functionalized with highly photoreactive minerals, thereby enabling co-localization of the photocatalytic units with a bacteria-based hydrogenase reaction for a successful semi-solid artificial photosynthesis system for hydrogen evolution.
34691703	2	67	theme	diatom	319:324	arg1	frustules					326:334	diatom frustules	319:334	diatom frustules	319:334	Recapitulating the remarkable architectures and functions of diatom frustules in artificial materials is a major challenge that has important technological implications for hierarchically ordered composites.
34691703	3	68	theme	fusion	646:651	arg1	proteins					653:660	genetically engineered amyloid fusion proteins	615:660	genetically engineered amyloid fusion proteins	615:660	Here, we report the construction of highly ordered, mineralized composites based on fabrication of complex self-supporting porous structures-made of genetically engineered amyloid fusion proteins and the natural polysaccharide chitin-and performing in situ multiscale protein-mediated mineralization with diverse inorganic materials, including SiO2, TiO2 and Ga2O3.
32268529	2	0	from	choices	422:428	arg1	conditions					457:466	varying conditions	449:466	varying conditions	449:466	It is of great importance to understand the hygroscopic behavior of WAW in different deterioration states for the development of optimal drying processes and choices of safe storage in varying conditions.
32268529	2	1	theme	deterioration	349:361	arg1	states					363:368	different deterioration states	339:368	different deterioration states for the development of optimal drying processes and choices of safe storage in varying conditions	339:466	It is of great importance to understand the hygroscopic behavior of WAW in different deterioration states for the development of optimal drying processes and choices of safe storage in varying conditions.
32268529	4	2	theme	WAW	639:641	arg1	state					630:634	The deterioration state	612:634	The deterioration state of WAW	612:641	The deterioration state of WAW was evaluated by the maximum water content (MWC) method and by the cell morphological structure.
32268529	7	3	theme	walls	1083:1087	arg1	analysis					1022:1029	Compositional analysis	1008:1029	Compositional analysis	1008:1029	Compositional analysis and hydroxyl accessibility measurements of WAW cell walls were shown to correlate with the hygroscopicity of WAW in different deterioration states.
32268529	7	3	theme	walls	1083:1087	arg1	measurements					1058:1069	hydroxyl accessibility measurements	1035:1069	hydroxyl accessibility measurements	1035:1069	Compositional analysis and hydroxyl accessibility measurements of WAW cell walls were shown to correlate with the hygroscopicity of WAW in different deterioration states.
32268529	9	4	dep	pretreatments	1655:1667	arg1	i.e.					1649:1652	i.e.	1649:1652	i.e.	1649:1652	This work helps in deciding on which consolidation measures are advised for shipwreck restauration, i.e., pretreatments with specific consolidates during wood drying, particularly for wooden artifacts displayed in museums.
32268529	1	5	theme	natural	175:181	arg1	polymers					197:204	natural biodegradable polymers	175:204	natural biodegradable polymers	175:204	Waterlogged archaeological wood (WAW) artifacts, made of natural biodegradable polymers, are important parts of many precious cultural heritages.
32268529	6	6	theme	hygroscopic	829:839	arg1	behavior					841:848	The hygroscopic behavior	825:848	The hygroscopic behavior of moderately and less decayed WAW	825:883	The hygroscopic behavior of moderately and less decayed WAW was then comparatively investigated using Dynamic Vapor Sorption (DVS) measurements alongside two sorption fitting models.
32268529	7	7	theme	WAW	1074:1076	arg1	walls					1083:1087	WAW cell walls	1074:1087	WAW cell walls	1074:1087	Compositional analysis and hydroxyl accessibility measurements of WAW cell walls were shown to correlate with the hygroscopicity of WAW in different deterioration states.
32268529	2	8	theme	hygroscopic	308:318	arg1	behavior					320:327	the hygroscopic behavior	304:327	the hygroscopic behavior of WAW in different deterioration states for the development of optimal drying processes and choices of safe storage in varying conditions	304:466	It is of great importance to understand the hygroscopic behavior of WAW in different deterioration states for the development of optimal drying processes and choices of safe storage in varying conditions.
32268529	6	9	theme	Vapor	935:939	arg1	measurements					956:967	Dynamic Vapor Sorption (DVS) measurements	927:967	Dynamic Vapor Sorption (DVS) measurements alongside two sorption fitting models	927:1005	The hygroscopic behavior of moderately and less decayed WAW was then comparatively investigated using Dynamic Vapor Sorption (DVS) measurements alongside two sorption fitting models.
32268529	9	10	theme	wood	1703:1706	arg1	drying					1708:1713	wood drying	1703:1713	wood drying	1703:1713	This work helps in deciding on which consolidation measures are advised for shipwreck restauration, i.e., pretreatments with specific consolidates during wood drying, particularly for wooden artifacts displayed in museums.
32268529	8	11	theme	groups	1435:1440	arg1	hydrolysis					1412:1421	the slow hydrolysis	1403:1421	the slow hydrolysis of O-acetyl groups of xylan	1403:1449	It was concluded that moderately decayed WAW possessed higher hygroscopicity and hysteresis than less decayed WAW because of the lower relative content of polysaccharides and the higher relative content of lignin, including the slow hydrolysis of O-acetyl groups of xylan and the partial breakage of β-O-4 interlinks, accompanied by an increased hydroxyl accessibility.
32268529	8	11	theme	groups	1435:1440	arg1	breakage					1467:1474	the partial breakage	1455:1474	the partial breakage of β-O-4 interlinks	1455:1494	It was concluded that moderately decayed WAW possessed higher hygroscopicity and hysteresis than less decayed WAW because of the lower relative content of polysaccharides and the higher relative content of lignin, including the slow hydrolysis of O-acetyl groups of xylan and the partial breakage of β-O-4 interlinks, accompanied by an increased hydroxyl accessibility.
32268529	7	12	theme	hydroxyl	1035:1042	arg1	measurements					1058:1069	hydroxyl accessibility measurements	1035:1069	hydroxyl accessibility measurements	1035:1069	Compositional analysis and hydroxyl accessibility measurements of WAW cell walls were shown to correlate with the hygroscopicity of WAW in different deterioration states.
32268529	7	13	theme	WAW	1140:1142	arg1	hygroscopicity					1122:1135	the hygroscopicity	1118:1135	the hygroscopicity of WAW in different deterioration states	1118:1176	Compositional analysis and hydroxyl accessibility measurements of WAW cell walls were shown to correlate with the hygroscopicity of WAW in different deterioration states.
32268529	0	14	from	Shipwreck	72:80	arg1	Hygroscopicity					0:13	Hygroscopicity	0:13	Hygroscopicity of Waterlogged Archaeological Wood from Xiaobaijiao No.1 Shipwreck	0:80	Hygroscopicity of Waterlogged Archaeological Wood from Xiaobaijiao No.1 Shipwreck Related to Its Deterioration State.
32268529	1	15	theme	wood	145:148	arg1	artifacts					156:164	Waterlogged archaeological wood (WAW) artifacts	118:164	Waterlogged archaeological wood (WAW) artifacts	118:164	Waterlogged archaeological wood (WAW) artifacts, made of natural biodegradable polymers, are important parts of many precious cultural heritages.
32268529	1	15	theme	wood	145:148	arg1	parts					221:225	important parts	211:225	important parts of many precious cultural heritages	211:261	Waterlogged archaeological wood (WAW) artifacts, made of natural biodegradable polymers, are important parts of many precious cultural heritages.
32268529	1	16	theme	WAW	151:153	arg1	artifacts					156:164	Waterlogged archaeological wood (WAW) artifacts	118:164	Waterlogged archaeological wood (WAW) artifacts	118:164	Waterlogged archaeological wood (WAW) artifacts, made of natural biodegradable polymers, are important parts of many precious cultural heritages.
32268529	1	16	theme	WAW	151:153	arg1	parts					221:225	important parts	211:225	important parts of many precious cultural heritages	211:261	Waterlogged archaeological wood (WAW) artifacts, made of natural biodegradable polymers, are important parts of many precious cultural heritages.
32268529	6	17	theme	sorption	983:990	arg1	models					1000:1005	two sorption fitting models	979:1005	two sorption fitting models	979:1005	The hygroscopic behavior of moderately and less decayed WAW was then comparatively investigated using Dynamic Vapor Sorption (DVS) measurements alongside two sorption fitting models.
32268529	8	18	theme	lower	1308:1312	arg1	content					1323:1329	the lower relative content	1304:1329	the lower relative content of polysaccharides and the higher relative content of lignin, including the slow hydrolysis of O-acetyl groups of xylan and the partial breakage of β-O-4 interlinks	1304:1494	It was concluded that moderately decayed WAW possessed higher hygroscopicity and hysteresis than less decayed WAW because of the lower relative content of polysaccharides and the higher relative content of lignin, including the slow hydrolysis of O-acetyl groups of xylan and the partial breakage of β-O-4 interlinks, accompanied by an increased hydroxyl accessibility.
32268529	8	19	theme	β-O-4	1479:1483	arg1	interlinks					1485:1494	β-O-4 interlinks	1479:1494	β-O-4 interlinks	1479:1494	It was concluded that moderately decayed WAW possessed higher hygroscopicity and hysteresis than less decayed WAW because of the lower relative content of polysaccharides and the higher relative content of lignin, including the slow hydrolysis of O-acetyl groups of xylan and the partial breakage of β-O-4 interlinks, accompanied by an increased hydroxyl accessibility.
32268529	4	20	theme	water	672:676	arg1	MWC					687:689	MWC	687:689	MWC	687:689	The deterioration state of WAW was evaluated by the maximum water content (MWC) method and by the cell morphological structure.
32268529	4	20	theme	water	672:676	arg1	content					678:684	maximum water content	664:684	the maximum water content (MWC) method	660:697	The deterioration state of WAW was evaluated by the maximum water content (MWC) method and by the cell morphological structure.
32268529	2	21	from	behavior	320:327	arg1	states					363:368	different deterioration states	339:368	different deterioration states for the development of optimal drying processes and choices of safe storage in varying conditions	339:466	It is of great importance to understand the hygroscopic behavior of WAW in different deterioration states for the development of optimal drying processes and choices of safe storage in varying conditions.
32268529	4	22	theme	morphological	715:727	arg1	structure					729:737	the cell morphological structure	706:737	the cell morphological structure	706:737	The deterioration state of WAW was evaluated by the maximum water content (MWC) method and by the cell morphological structure.
32268529	0	23	theme	Deterioration	97:109	arg1	State					111:115	Its Deterioration State	93:115	Its Deterioration State	93:115	Hygroscopicity of Waterlogged Archaeological Wood from Xiaobaijiao No.1 Shipwreck Related to Its Deterioration State.
32268529	2	24	from	conditions	457:466	arg1	choices					422:428	choices	422:428	choices of safe storage in varying conditions	422:466	It is of great importance to understand the hygroscopic behavior of WAW in different deterioration states for the development of optimal drying processes and choices of safe storage in varying conditions.
32268529	2	24	from	conditions	457:466	arg1	processes					408:416	optimal drying processes	393:416	optimal drying processes	393:416	It is of great importance to understand the hygroscopic behavior of WAW in different deterioration states for the development of optimal drying processes and choices of safe storage in varying conditions.
32268529	2	25	theme	drying	401:406	arg1	processes					408:416	optimal drying processes	393:416	optimal drying processes	393:416	It is of great importance to understand the hygroscopic behavior of WAW in different deterioration states for the development of optimal drying processes and choices of safe storage in varying conditions.
32268529	2	26	theme	varying	449:455	arg1	conditions					457:466	varying conditions	449:466	varying conditions	449:466	It is of great importance to understand the hygroscopic behavior of WAW in different deterioration states for the development of optimal drying processes and choices of safe storage in varying conditions.
32268529	8	27	theme	increased	1515:1523	arg1	accessibility					1534:1546	an increased hydroxyl accessibility	1512:1546	an increased hydroxyl accessibility	1512:1546	It was concluded that moderately decayed WAW possessed higher hygroscopicity and hysteresis than less decayed WAW because of the lower relative content of polysaccharides and the higher relative content of lignin, including the slow hydrolysis of O-acetyl groups of xylan and the partial breakage of β-O-4 interlinks, accompanied by an increased hydroxyl accessibility.
32268529	8	28	theme	relative	1365:1372	arg1	content					1374:1380	the higher relative content	1354:1380	the higher relative content	1354:1380	It was concluded that moderately decayed WAW possessed higher hygroscopicity and hysteresis than less decayed WAW because of the lower relative content of polysaccharides and the higher relative content of lignin, including the slow hydrolysis of O-acetyl groups of xylan and the partial breakage of β-O-4 interlinks, accompanied by an increased hydroxyl accessibility.
32268529	7	29	theme	deterioration	1157:1169	arg1	states					1171:1176	different deterioration states	1147:1176	different deterioration states	1147:1176	Compositional analysis and hydroxyl accessibility measurements of WAW cell walls were shown to correlate with the hygroscopicity of WAW in different deterioration states.
32268529	2	30	theme	storage	438:444	arg1	choices					422:428	choices	422:428	choices of safe storage in varying conditions	422:466	It is of great importance to understand the hygroscopic behavior of WAW in different deterioration states for the development of optimal drying processes and choices of safe storage in varying conditions.
32268529	2	30	theme	storage	438:444	arg1	processes					408:416	optimal drying processes	393:416	optimal drying processes	393:416	It is of great importance to understand the hygroscopic behavior of WAW in different deterioration states for the development of optimal drying processes and choices of safe storage in varying conditions.
32268529	0	31	theme	Waterlogged	18:28	arg1	Wood					45:48	Waterlogged Archaeological Wood	18:48	Waterlogged Archaeological Wood	18:48	Hygroscopicity of Waterlogged Archaeological Wood from Xiaobaijiao No.1 Shipwreck Related to Its Deterioration State.
32268529	3	32	theme	Giam	524:527	arg1	samples					557:563	two Hopea (Giam) and two Tectona (Teak) WAW samples	513:563	two Hopea (Giam) and two Tectona (Teak) WAW samples collected from the Xiaobaijiao No.1 shipwreck	513:609	This was investigated in a case-study using two Hopea (Giam) and two Tectona (Teak) WAW samples collected from the Xiaobaijiao No.1 shipwreck.
32268529	1	33	theme	Waterlogged	118:128	arg1	artifacts					156:164	Waterlogged archaeological wood (WAW) artifacts	118:164	Waterlogged archaeological wood (WAW) artifacts	118:164	Waterlogged archaeological wood (WAW) artifacts, made of natural biodegradable polymers, are important parts of many precious cultural heritages.
32268529	1	33	theme	Waterlogged	118:128	arg1	parts					221:225	important parts	211:225	important parts of many precious cultural heritages	211:261	Waterlogged archaeological wood (WAW) artifacts, made of natural biodegradable polymers, are important parts of many precious cultural heritages.
32268529	0	34	theme	Wood	45:48	arg1	Hygroscopicity					0:13	Hygroscopicity	0:13	Hygroscopicity of Waterlogged Archaeological Wood from Xiaobaijiao No.1 Shipwreck	0:80	Hygroscopicity of Waterlogged Archaeological Wood from Xiaobaijiao No.1 Shipwreck Related to Its Deterioration State.
32268529	9	35	theme	consolidation	1586:1598	arg1	measures					1600:1607	consolidation measures	1586:1607	consolidation measures	1586:1607	This work helps in deciding on which consolidation measures are advised for shipwreck restauration, i.e., pretreatments with specific consolidates during wood drying, particularly for wooden artifacts displayed in museums.
32268529	2	36	theme	different	339:347	arg1	states					363:368	different deterioration states	339:368	different deterioration states for the development of optimal drying processes and choices of safe storage in varying conditions	339:466	It is of great importance to understand the hygroscopic behavior of WAW in different deterioration states for the development of optimal drying processes and choices of safe storage in varying conditions.
32268529	9	37	dep	specific	1674:1681	arg1	consolidates					1683:1694	consolidates	1683:1694	consolidates	1683:1694	This work helps in deciding on which consolidation measures are advised for shipwreck restauration, i.e., pretreatments with specific consolidates during wood drying, particularly for wooden artifacts displayed in museums.
32268529	2	38	from	storage	438:444	arg1	conditions					457:466	varying conditions	449:466	varying conditions	449:466	It is of great importance to understand the hygroscopic behavior of WAW in different deterioration states for the development of optimal drying processes and choices of safe storage in varying conditions.
32268529	2	39	theme	WAW	332:334	arg1	behavior					320:327	the hygroscopic behavior	304:327	the hygroscopic behavior of WAW in different deterioration states for the development of optimal drying processes and choices of safe storage in varying conditions	304:466	It is of great importance to understand the hygroscopic behavior of WAW in different deterioration states for the development of optimal drying processes and choices of safe storage in varying conditions.
32268529	8	40	theme	O-acetyl	1426:1433	arg1	xylan					1445:1449	xylan	1445:1449	xylan	1445:1449	It was concluded that moderately decayed WAW possessed higher hygroscopicity and hysteresis than less decayed WAW because of the lower relative content of polysaccharides and the higher relative content of lignin, including the slow hydrolysis of O-acetyl groups of xylan and the partial breakage of β-O-4 interlinks, accompanied by an increased hydroxyl accessibility.
32268529	8	40	theme	O-acetyl	1426:1433	arg1	groups					1435:1440	O-acetyl groups	1426:1440	O-acetyl groups of xylan	1426:1449	It was concluded that moderately decayed WAW possessed higher hygroscopicity and hysteresis than less decayed WAW because of the lower relative content of polysaccharides and the higher relative content of lignin, including the slow hydrolysis of O-acetyl groups of xylan and the partial breakage of β-O-4 interlinks, accompanied by an increased hydroxyl accessibility.
32268529	1	41	theme	precious	235:242	arg1	heritages					253:261	many precious cultural heritages	230:261	many precious cultural heritages	230:261	Waterlogged archaeological wood (WAW) artifacts, made of natural biodegradable polymers, are important parts of many precious cultural heritages.
32268529	1	42	theme	heritages	253:261	arg1	artifacts					156:164	Waterlogged archaeological wood (WAW) artifacts	118:164	Waterlogged archaeological wood (WAW) artifacts	118:164	Waterlogged archaeological wood (WAW) artifacts, made of natural biodegradable polymers, are important parts of many precious cultural heritages.
32268529	1	42	theme	heritages	253:261	arg1	parts					221:225	important parts	211:225	important parts of many precious cultural heritages	211:261	Waterlogged archaeological wood (WAW) artifacts, made of natural biodegradable polymers, are important parts of many precious cultural heritages.
32268529	6	43	theme	less	868:871	arg1	behavior					841:848	The hygroscopic behavior	825:848	The hygroscopic behavior of moderately and less decayed WAW	825:883	The hygroscopic behavior of moderately and less decayed WAW was then comparatively investigated using Dynamic Vapor Sorption (DVS) measurements alongside two sorption fitting models.
32268529	6	44	theme	moderately	853:862	arg1	behavior					841:848	The hygroscopic behavior	825:848	The hygroscopic behavior of moderately and less decayed WAW	825:883	The hygroscopic behavior of moderately and less decayed WAW was then comparatively investigated using Dynamic Vapor Sorption (DVS) measurements alongside two sorption fitting models.
32268529	7	45	theme	cell	1078:1081	arg1	walls					1083:1087	WAW cell walls	1074:1087	WAW cell walls	1074:1087	Compositional analysis and hydroxyl accessibility measurements of WAW cell walls were shown to correlate with the hygroscopicity of WAW in different deterioration states.
32268529	2	46	theme	great	273:277	arg1	importance					279:288	great importance	273:288	great importance	273:288	It is of great importance to understand the hygroscopic behavior of WAW in different deterioration states for the development of optimal drying processes and choices of safe storage in varying conditions.
32268529	1	47	theme	biodegradable	183:195	arg1	polymers					197:204	natural biodegradable polymers	175:204	natural biodegradable polymers	175:204	Waterlogged archaeological wood (WAW) artifacts, made of natural biodegradable polymers, are important parts of many precious cultural heritages.
32268529	6	48	theme	Sorption	941:948	arg1	measurements					956:967	Dynamic Vapor Sorption (DVS) measurements	927:967	Dynamic Vapor Sorption (DVS) measurements alongside two sorption fitting models	927:1005	The hygroscopic behavior of moderately and less decayed WAW was then comparatively investigated using Dynamic Vapor Sorption (DVS) measurements alongside two sorption fitting models.
32268529	8	49	theme	higher	1234:1239	arg1	hygroscopicity					1241:1254	higher hygroscopicity	1234:1254	higher hygroscopicity	1234:1254	It was concluded that moderately decayed WAW possessed higher hygroscopicity and hysteresis than less decayed WAW because of the lower relative content of polysaccharides and the higher relative content of lignin, including the slow hydrolysis of O-acetyl groups of xylan and the partial breakage of β-O-4 interlinks, accompanied by an increased hydroxyl accessibility.
32268529	7	50	theme	accessibility	1044:1056	arg1	measurements					1058:1069	hydroxyl accessibility measurements	1035:1069	hydroxyl accessibility measurements	1035:1069	Compositional analysis and hydroxyl accessibility measurements of WAW cell walls were shown to correlate with the hygroscopicity of WAW in different deterioration states.
32268529	3	51	theme	Tectona	538:544	arg1	samples					557:563	two Hopea (Giam) and two Tectona (Teak) WAW samples	513:563	two Hopea (Giam) and two Tectona (Teak) WAW samples collected from the Xiaobaijiao No.1 shipwreck	513:609	This was investigated in a case-study using two Hopea (Giam) and two Tectona (Teak) WAW samples collected from the Xiaobaijiao No.1 shipwreck.
32268529	6	52	theme	Dynamic	927:933	arg1	measurements					956:967	Dynamic Vapor Sorption (DVS) measurements	927:967	Dynamic Vapor Sorption (DVS) measurements alongside two sorption fitting models	927:1005	The hygroscopic behavior of moderately and less decayed WAW was then comparatively investigated using Dynamic Vapor Sorption (DVS) measurements alongside two sorption fitting models.
32268529	4	53	theme	maximum	664:670	arg1	MWC					687:689	MWC	687:689	MWC	687:689	The deterioration state of WAW was evaluated by the maximum water content (MWC) method and by the cell morphological structure.
32268529	4	53	theme	maximum	664:670	arg1	content					678:684	maximum water content	664:684	the maximum water content (MWC) method	660:697	The deterioration state of WAW was evaluated by the maximum water content (MWC) method and by the cell morphological structure.
32268529	1	54	theme	important	211:219	arg1	artifacts					156:164	Waterlogged archaeological wood (WAW) artifacts	118:164	Waterlogged archaeological wood (WAW) artifacts	118:164	Waterlogged archaeological wood (WAW) artifacts, made of natural biodegradable polymers, are important parts of many precious cultural heritages.
32268529	1	54	theme	important	211:219	arg1	parts					221:225	important parts	211:225	important parts of many precious cultural heritages	211:261	Waterlogged archaeological wood (WAW) artifacts, made of natural biodegradable polymers, are important parts of many precious cultural heritages.
32268529	4	55	theme	deterioration	616:628	arg1	state					630:634	The deterioration state	612:634	The deterioration state of WAW	612:641	The deterioration state of WAW was evaluated by the maximum water content (MWC) method and by the cell morphological structure.
32268529	7	56	theme	Compositional	1008:1020	arg1	analysis					1022:1029	Compositional analysis	1008:1029	Compositional analysis	1008:1029	Compositional analysis and hydroxyl accessibility measurements of WAW cell walls were shown to correlate with the hygroscopicity of WAW in different deterioration states.
32268529	3	57	theme	WAW	553:555	arg1	samples					557:563	two Hopea (Giam) and two Tectona (Teak) WAW samples	513:563	two Hopea (Giam) and two Tectona (Teak) WAW samples collected from the Xiaobaijiao No.1 shipwreck	513:609	This was investigated in a case-study using two Hopea (Giam) and two Tectona (Teak) WAW samples collected from the Xiaobaijiao No.1 shipwreck.
32268529	1	58	theme	archaeological	130:143	arg1	artifacts					156:164	Waterlogged archaeological wood (WAW) artifacts	118:164	Waterlogged archaeological wood (WAW) artifacts	118:164	Waterlogged archaeological wood (WAW) artifacts, made of natural biodegradable polymers, are important parts of many precious cultural heritages.
32268529	1	58	theme	archaeological	130:143	arg1	parts					221:225	important parts	211:225	important parts of many precious cultural heritages	211:261	Waterlogged archaeological wood (WAW) artifacts, made of natural biodegradable polymers, are important parts of many precious cultural heritages.
32268529	9	59	theme	wooden	1733:1738	arg1	artifacts					1740:1748	wooden artifacts	1733:1748	wooden artifacts displayed in museums	1733:1769	This work helps in deciding on which consolidation measures are advised for shipwreck restauration, i.e., pretreatments with specific consolidates during wood drying, particularly for wooden artifacts displayed in museums.
32268529	8	60	theme	partial	1459:1465	arg1	breakage					1467:1474	the partial breakage	1455:1474	the partial breakage of β-O-4 interlinks	1455:1494	It was concluded that moderately decayed WAW possessed higher hygroscopicity and hysteresis than less decayed WAW because of the lower relative content of polysaccharides and the higher relative content of lignin, including the slow hydrolysis of O-acetyl groups of xylan and the partial breakage of β-O-4 interlinks, accompanied by an increased hydroxyl accessibility.
32268529	4	61	theme	content	678:684	arg1	method					692:697	the maximum water content (MWC) method	660:697	the maximum water content (MWC) method	660:697	The deterioration state of WAW was evaluated by the maximum water content (MWC) method and by the cell morphological structure.
32268529	6	62	theme	fitting	992:998	arg1	models					1000:1005	two sorption fitting models	979:1005	two sorption fitting models	979:1005	The hygroscopic behavior of moderately and less decayed WAW was then comparatively investigated using Dynamic Vapor Sorption (DVS) measurements alongside two sorption fitting models.
32268529	8	63	theme	polysaccharides	1334:1348	arg1	content					1323:1329	the lower relative content	1304:1329	the lower relative content of polysaccharides and the higher relative content of lignin, including the slow hydrolysis of O-acetyl groups of xylan and the partial breakage of β-O-4 interlinks	1304:1494	It was concluded that moderately decayed WAW possessed higher hygroscopicity and hysteresis than less decayed WAW because of the lower relative content of polysaccharides and the higher relative content of lignin, including the slow hydrolysis of O-acetyl groups of xylan and the partial breakage of β-O-4 interlinks, accompanied by an increased hydroxyl accessibility.
32268529	8	64	theme	interlinks	1485:1494	arg1	hydrolysis					1412:1421	the slow hydrolysis	1403:1421	the slow hydrolysis of O-acetyl groups of xylan	1403:1449	It was concluded that moderately decayed WAW possessed higher hygroscopicity and hysteresis than less decayed WAW because of the lower relative content of polysaccharides and the higher relative content of lignin, including the slow hydrolysis of O-acetyl groups of xylan and the partial breakage of β-O-4 interlinks, accompanied by an increased hydroxyl accessibility.
32268529	8	64	theme	interlinks	1485:1494	arg1	breakage					1467:1474	the partial breakage	1455:1474	the partial breakage of β-O-4 interlinks	1455:1494	It was concluded that moderately decayed WAW possessed higher hygroscopicity and hysteresis than less decayed WAW because of the lower relative content of polysaccharides and the higher relative content of lignin, including the slow hydrolysis of O-acetyl groups of xylan and the partial breakage of β-O-4 interlinks, accompanied by an increased hydroxyl accessibility.
32268529	8	65	theme	xylan	1445:1449	arg1	xylan					1445:1449	xylan	1445:1449	xylan	1445:1449	It was concluded that moderately decayed WAW possessed higher hygroscopicity and hysteresis than less decayed WAW because of the lower relative content of polysaccharides and the higher relative content of lignin, including the slow hydrolysis of O-acetyl groups of xylan and the partial breakage of β-O-4 interlinks, accompanied by an increased hydroxyl accessibility.
32268529	8	65	theme	xylan	1445:1449	arg1	groups					1435:1440	O-acetyl groups	1426:1440	O-acetyl groups of xylan	1426:1449	It was concluded that moderately decayed WAW possessed higher hygroscopicity and hysteresis than less decayed WAW because of the lower relative content of polysaccharides and the higher relative content of lignin, including the slow hydrolysis of O-acetyl groups of xylan and the partial breakage of β-O-4 interlinks, accompanied by an increased hydroxyl accessibility.
32268529	7	66	from	hygroscopicity	1122:1135	arg1	states					1171:1176	different deterioration states	1147:1176	different deterioration states	1147:1176	Compositional analysis and hydroxyl accessibility measurements of WAW cell walls were shown to correlate with the hygroscopicity of WAW in different deterioration states.
32268529	8	67	theme	relative	1314:1321	arg1	content					1323:1329	the lower relative content	1304:1329	the lower relative content of polysaccharides and the higher relative content of lignin, including the slow hydrolysis of O-acetyl groups of xylan and the partial breakage of β-O-4 interlinks	1304:1494	It was concluded that moderately decayed WAW possessed higher hygroscopicity and hysteresis than less decayed WAW because of the lower relative content of polysaccharides and the higher relative content of lignin, including the slow hydrolysis of O-acetyl groups of xylan and the partial breakage of β-O-4 interlinks, accompanied by an increased hydroxyl accessibility.
32268529	6	68	theme	DVS	951:953	arg1	measurements					956:967	Dynamic Vapor Sorption (DVS) measurements	927:967	Dynamic Vapor Sorption (DVS) measurements alongside two sorption fitting models	927:1005	The hygroscopic behavior of moderately and less decayed WAW was then comparatively investigated using Dynamic Vapor Sorption (DVS) measurements alongside two sorption fitting models.
32268529	4	69	theme	cell	710:713	arg1	structure					729:737	the cell morphological structure	706:737	the cell morphological structure	706:737	The deterioration state of WAW was evaluated by the maximum water content (MWC) method and by the cell morphological structure.
32268529	8	70	theme	hydroxyl	1525:1532	arg1	accessibility					1534:1546	an increased hydroxyl accessibility	1512:1546	an increased hydroxyl accessibility	1512:1546	It was concluded that moderately decayed WAW possessed higher hygroscopicity and hysteresis than less decayed WAW because of the lower relative content of polysaccharides and the higher relative content of lignin, including the slow hydrolysis of O-acetyl groups of xylan and the partial breakage of β-O-4 interlinks, accompanied by an increased hydroxyl accessibility.
32268529	2	71	theme	optimal	393:399	arg1	processes					408:416	optimal drying processes	393:416	optimal drying processes	393:416	It is of great importance to understand the hygroscopic behavior of WAW in different deterioration states for the development of optimal drying processes and choices of safe storage in varying conditions.
32268529	7	72	theme	different	1147:1155	arg1	states					1171:1176	different deterioration states	1147:1176	different deterioration states	1147:1176	Compositional analysis and hydroxyl accessibility measurements of WAW cell walls were shown to correlate with the hygroscopicity of WAW in different deterioration states.
32268529	8	73	theme	higher	1358:1363	arg1	content					1374:1380	the higher relative content	1354:1380	the higher relative content	1354:1380	It was concluded that moderately decayed WAW possessed higher hygroscopicity and hysteresis than less decayed WAW because of the lower relative content of polysaccharides and the higher relative content of lignin, including the slow hydrolysis of O-acetyl groups of xylan and the partial breakage of β-O-4 interlinks, accompanied by an increased hydroxyl accessibility.
32268529	0	74	theme	Archaeological	30:43	arg1	Wood					45:48	Waterlogged Archaeological Wood	18:48	Waterlogged Archaeological Wood	18:48	Hygroscopicity of Waterlogged Archaeological Wood from Xiaobaijiao No.1 Shipwreck Related to Its Deterioration State.
32268529	2	75	theme	safe	433:436	arg1	storage					438:444	safe storage	433:444	safe storage in varying conditions	433:466	It is of great importance to understand the hygroscopic behavior of WAW in different deterioration states for the development of optimal drying processes and choices of safe storage in varying conditions.
32268529	8	76	theme	content	1374:1380	arg1	content					1323:1329	the lower relative content	1304:1329	the lower relative content of polysaccharides and the higher relative content of lignin, including the slow hydrolysis of O-acetyl groups of xylan and the partial breakage of β-O-4 interlinks	1304:1494	It was concluded that moderately decayed WAW possessed higher hygroscopicity and hysteresis than less decayed WAW because of the lower relative content of polysaccharides and the higher relative content of lignin, including the slow hydrolysis of O-acetyl groups of xylan and the partial breakage of β-O-4 interlinks, accompanied by an increased hydroxyl accessibility.
32268529	2	77	theme	choices	422:428	arg1	development					378:388	the development	374:388	the development of optimal drying processes and choices of safe storage in varying conditions	374:466	It is of great importance to understand the hygroscopic behavior of WAW in different deterioration states for the development of optimal drying processes and choices of safe storage in varying conditions.
32268529	8	78	theme	lignin	1385:1390	arg1	hydrolysis					1412:1421	the slow hydrolysis	1403:1421	the slow hydrolysis of O-acetyl groups of xylan	1403:1449	It was concluded that moderately decayed WAW possessed higher hygroscopicity and hysteresis than less decayed WAW because of the lower relative content of polysaccharides and the higher relative content of lignin, including the slow hydrolysis of O-acetyl groups of xylan and the partial breakage of β-O-4 interlinks, accompanied by an increased hydroxyl accessibility.
32268529	8	78	theme	lignin	1385:1390	arg1	content					1374:1380	the higher relative content	1354:1380	the higher relative content	1354:1380	It was concluded that moderately decayed WAW possessed higher hygroscopicity and hysteresis than less decayed WAW because of the lower relative content of polysaccharides and the higher relative content of lignin, including the slow hydrolysis of O-acetyl groups of xylan and the partial breakage of β-O-4 interlinks, accompanied by an increased hydroxyl accessibility.
32268529	8	78	theme	lignin	1385:1390	arg1	polysaccharides					1334:1348	polysaccharides	1334:1348	polysaccharides	1334:1348	It was concluded that moderately decayed WAW possessed higher hygroscopicity and hysteresis than less decayed WAW because of the lower relative content of polysaccharides and the higher relative content of lignin, including the slow hydrolysis of O-acetyl groups of xylan and the partial breakage of β-O-4 interlinks, accompanied by an increased hydroxyl accessibility.
32268529	8	78	theme	lignin	1385:1390	arg1	breakage					1467:1474	the partial breakage	1455:1474	the partial breakage of β-O-4 interlinks	1455:1494	It was concluded that moderately decayed WAW possessed higher hygroscopicity and hysteresis than less decayed WAW because of the lower relative content of polysaccharides and the higher relative content of lignin, including the slow hydrolysis of O-acetyl groups of xylan and the partial breakage of β-O-4 interlinks, accompanied by an increased hydroxyl accessibility.
32268529	2	79	theme	processes	408:416	arg1	development					378:388	the development	374:388	the development of optimal drying processes and choices of safe storage in varying conditions	374:466	It is of great importance to understand the hygroscopic behavior of WAW in different deterioration states for the development of optimal drying processes and choices of safe storage in varying conditions.
32268529	8	80	theme	slow	1407:1410	arg1	hydrolysis					1412:1421	the slow hydrolysis	1403:1421	the slow hydrolysis of O-acetyl groups of xylan	1403:1449	It was concluded that moderately decayed WAW possessed higher hygroscopicity and hysteresis than less decayed WAW because of the lower relative content of polysaccharides and the higher relative content of lignin, including the slow hydrolysis of O-acetyl groups of xylan and the partial breakage of β-O-4 interlinks, accompanied by an increased hydroxyl accessibility.
32268529	1	81	theme	many	230:233	arg1	heritages					253:261	many precious cultural heritages	230:261	many precious cultural heritages	230:261	Waterlogged archaeological wood (WAW) artifacts, made of natural biodegradable polymers, are important parts of many precious cultural heritages.
32268529	9	82	theme	shipwreck	1625:1633	arg1	restauration					1635:1646	shipwreck restauration	1625:1646	shipwreck restauration	1625:1646	This work helps in deciding on which consolidation measures are advised for shipwreck restauration, i.e., pretreatments with specific consolidates during wood drying, particularly for wooden artifacts displayed in museums.
32268529	2	83	from	processes	408:416	arg1	conditions					457:466	varying conditions	449:466	varying conditions	449:466	It is of great importance to understand the hygroscopic behavior of WAW in different deterioration states for the development of optimal drying processes and choices of safe storage in varying conditions.
32268529	9	84	with	pretreatments	1655:1667	arg1	specific					1674:1681	specific	1674:1681	specific	1674:1681	This work helps in deciding on which consolidation measures are advised for shipwreck restauration, i.e., pretreatments with specific consolidates during wood drying, particularly for wooden artifacts displayed in museums.
32268529	1	85	theme	cultural	244:251	arg1	heritages					253:261	many precious cultural heritages	230:261	many precious cultural heritages	230:261	Waterlogged archaeological wood (WAW) artifacts, made of natural biodegradable polymers, are important parts of many precious cultural heritages.
34634365	3	0	theme	inflammatory	661:672	arg1	rats					674:677	lipopolysaccharide (LPS)-induced inflammatory rats	628:677	lipopolysaccharide (LPS)-induced inflammatory rats	628:677	AIMS OF THE STUDY To reveal the effect of YN on gut microbiota dysbiosis, and explore the potential role of the gut microecosystem and CD4+ T cell immune homeostasis in YN-treated respiratory and intestinal diseases in lipopolysaccharide (LPS)-induced inflammatory rats.
34634365	7	1	theme	cytokine	1094:1101	arg1	profiles					1103:1110	the serum and colon cytokine profiles	1074:1110	the serum and colon cytokine profiles	1074:1110	A Luminex cytokine microarray and enzyme-linked immunosorbent assay (ELISA) were conducted to evaluate the serum and colon cytokine profiles.
34634365	8	2	theme	immune	1132:1137	arg1	blood					1270:1274	whole blood	1264:1274	whole blood	1264:1274	The frequencies of immune cells, including Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs), bronchoalveolar lavage fluid (BALF) and whole blood were phenotyped using flow cytometry.
34634365	8	2	theme	immune	1132:1137	arg1	cells					1180:1184	Th1, Th2, Th17 and Treg cells	1156:1184	Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs)	1156:1221	The frequencies of immune cells, including Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs), bronchoalveolar lavage fluid (BALF) and whole blood were phenotyped using flow cytometry.
34634365	8	2	theme	immune	1132:1137	arg1	cells					1139:1143	immune cells	1132:1143	immune cells	1132:1143	The frequencies of immune cells, including Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs), bronchoalveolar lavage fluid (BALF) and whole blood were phenotyped using flow cytometry.
34634365	8	2	theme	immune	1132:1137	arg1	fluid					1247:1251	bronchoalveolar lavage fluid	1224:1251	bronchoalveolar lavage fluid (BALF)	1224:1258	The frequencies of immune cells, including Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs), bronchoalveolar lavage fluid (BALF) and whole blood were phenotyped using flow cytometry.
34634365	6	3	theme	acid	876:879	arg1	production					888:897	short-chain fatty acid (SCFA) production	858:897	short-chain fatty acid (SCFA) production	858:897	Changes in gut microbiota and short-chain fatty acid (SCFA) production were analysed using 16S rRNA gene sequencing and targeted metabolomics.
34634365	10	4	theme	IL-1α	1704:1708	arg1	levels					1694:1699	the levels	1690:1699	the levels of IL-1α, IL-5, IL-7, IL-12 (p70), GM-CSF and VEGF	1690:1750	Notably, YN was found to improve the immunosuppressed state induced by LPS in rats, which not only upregulated the levels of the proinflammatory cytokine IL-17A and the immunosuppressive cytokines IL-4 and IL-10 in colon tissue but also increased the levels of IL-1α, IL-5, IL-7, IL-12 (p70), GM-CSF and VEGF in serum.
34634365	3	5	from	diseases	616:623	arg1	rats					674:677	lipopolysaccharide (LPS)-induced inflammatory rats	628:677	lipopolysaccharide (LPS)-induced inflammatory rats	628:677	AIMS OF THE STUDY To reveal the effect of YN on gut microbiota dysbiosis, and explore the potential role of the gut microecosystem and CD4+ T cell immune homeostasis in YN-treated respiratory and intestinal diseases in lipopolysaccharide (LPS)-induced inflammatory rats.
34634365	16	6	theme	Treg/Th17 cells	2789:2803	arg1	balance					2766:2772	the balance	2762:2772	the balance of Th1/Th2 and Treg/Th17 cells	2762:2803	CONCLUSIONS These results demonstrated that YN could ameliorate LPS-induced inflammation in rats by modifying gut microbiota, increasing microbiota-derived SCFA production and regulating the balance of Th1/Th2 and Treg/Th17 cells.
34634365	11	7	theme	rats	1841:1844	arg1	Th17 cells					1777:1786	Th17 cells	1777:1786	Th17 cells	1777:1786	The numbers of Th17 cells and Treg cells in the MLNs, blood, and BALF of model rats were regulated by YN, with the restoration of the Th17/Treg balance.
34634365	11	7	theme	rats	1841:1844	arg1	BALF					1827:1830	BALF	1827:1830	BALF	1827:1830	The numbers of Th17 cells and Treg cells in the MLNs, blood, and BALF of model rats were regulated by YN, with the restoration of the Th17/Treg balance.
34634365	11	7	theme	rats	1841:1844	arg1	cells					1797:1801	Treg cells	1792:1801	Treg cells	1792:1801	The numbers of Th17 cells and Treg cells in the MLNs, blood, and BALF of model rats were regulated by YN, with the restoration of the Th17/Treg balance.
34634365	11	7	theme	rats	1841:1844	arg1	blood					1816:1820	blood	1816:1820	blood	1816:1820	The numbers of Th17 cells and Treg cells in the MLNs, blood, and BALF of model rats were regulated by YN, with the restoration of the Th17/Treg balance.
34634365	9	8	theme	colon	1354:1358	arg1	inflammation					1360:1371	less colon inflammation	1349:1371	less colon inflammation	1349:1371	RESULTS The YN-treated rats showed less colon inflammation, as evidenced by the reduction in mortality rate and histology score.
34634365	6	9	theme	short-chain	858:868	arg1	SCFA					882:885	SCFA	882:885	SCFA	882:885	Changes in gut microbiota and short-chain fatty acid (SCFA) production were analysed using 16S rRNA gene sequencing and targeted metabolomics.
34634365	6	9	theme	short-chain	858:868	arg1	acid					876:879	short-chain fatty acid	858:879	short-chain fatty acid (SCFA) production	858:897	Changes in gut microbiota and short-chain fatty acid (SCFA) production were analysed using 16S rRNA gene sequencing and targeted metabolomics.
34634365	11	10	theme	cells	1797:1801	arg1	numbers					1766:1772	The numbers	1762:1772	The numbers of Th17 cells and Treg cells in the MLNs, blood, and BALF of model rats	1762:1844	The numbers of Th17 cells and Treg cells in the MLNs, blood, and BALF of model rats were regulated by YN, with the restoration of the Th17/Treg balance.
34634365	11	11	from	blood	1816:1820	arg1	MLNs					1810:1813	the MLNs	1806:1813	the MLNs	1806:1813	The numbers of Th17 cells and Treg cells in the MLNs, blood, and BALF of model rats were regulated by YN, with the restoration of the Th17/Treg balance.
34634365	15	12	theme	dominant	2442:2449	arg1	genera					2461:2466	the dominant bacterial genera	2438:2466	the dominant bacterial genera regulated by YN administration	2438:2497	Most of the dominant bacterial genera regulated by YN administration were correlated with the concentrations of SCFA and inflammatory cytokines.
34634365	16	13	dep	CONCLUSIONS	2575:2585	arg1	demonstrated					2601:2612	demonstrated	2601:2612	demonstrated that YN could ameliorate LPS-induced inflammation in rats by modifying gut microbiota, increasing microbiota-derived SCFA production and regulating the balance of Th1/Th2 and Treg/Th17 cells	2601:2803	CONCLUSIONS These results demonstrated that YN could ameliorate LPS-induced inflammation in rats by modifying gut microbiota, increasing microbiota-derived SCFA production and regulating the balance of Th1/Th2 and Treg/Th17 cells.
34634365	8	14	theme	Th1	1156:1158	arg1	cells					1180:1184	Th1, Th2, Th17 and Treg cells	1156:1184	Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs)	1156:1221	The frequencies of immune cells, including Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs), bronchoalveolar lavage fluid (BALF) and whole blood were phenotyped using flow cytometry.
34634365	8	15	theme	mesenteric	1193:1202	arg1	nodes					1210:1214	the mesenteric lymph nodes	1189:1214	the mesenteric lymph nodes (MLNs)	1189:1221	The frequencies of immune cells, including Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs), bronchoalveolar lavage fluid (BALF) and whole blood were phenotyped using flow cytometry.
34634365	8	15	theme	mesenteric	1193:1202	arg1	MLNs					1217:1220	MLNs	1217:1220	MLNs	1217:1220	The frequencies of immune cells, including Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs), bronchoalveolar lavage fluid (BALF) and whole blood were phenotyped using flow cytometry.
34634365	3	16	theme	CD4+	544:547	arg1	cell					551:554	CD4+ T cell	544:554	CD4+ T cell	544:554	AIMS OF THE STUDY To reveal the effect of YN on gut microbiota dysbiosis, and explore the potential role of the gut microecosystem and CD4+ T cell immune homeostasis in YN-treated respiratory and intestinal diseases in lipopolysaccharide (LPS)-induced inflammatory rats.
34634365	16	17	from	inflammation	2651:2662	arg1	rats					2667:2670	rats	2667:2670	rats	2667:2670	CONCLUSIONS These results demonstrated that YN could ameliorate LPS-induced inflammation in rats by modifying gut microbiota, increasing microbiota-derived SCFA production and regulating the balance of Th1/Th2 and Treg/Th17 cells.
34634365	1	18	theme	commercial	191:200	arg1	medicine					217:224	a commercial Chinese patent medicine	189:224	a commercial Chinese patent medicine	189:224	ETHNOPHARMACOLOGICAL RELEVANCE As a commercial Chinese patent medicine, Yanning Syrup (YN) is used to treat acute upper respiratory tract infections and acute enteritis effectively in clinical practice.
34634365	1	18	theme	commercial	191:200	arg1	Syrup					235:239	Syrup	235:239	Syrup	235:239	ETHNOPHARMACOLOGICAL RELEVANCE As a commercial Chinese patent medicine, Yanning Syrup (YN) is used to treat acute upper respiratory tract infections and acute enteritis effectively in clinical practice.
34634365	8	19	theme	Th2	1161:1163	arg1	cells					1180:1184	Th1, Th2, Th17 and Treg cells	1156:1184	Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs)	1156:1221	The frequencies of immune cells, including Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs), bronchoalveolar lavage fluid (BALF) and whole blood were phenotyped using flow cytometry.
34634365	6	20	theme	gene	928:931	arg1	sequencing					933:942	16S rRNA gene sequencing	919:942	16S rRNA gene sequencing	919:942	Changes in gut microbiota and short-chain fatty acid (SCFA) production were analysed using 16S rRNA gene sequencing and targeted metabolomics.
34634365	16	21	theme	microbiota-derived	2712:2729	arg1	production					2736:2745	microbiota-derived SCFA production	2712:2745	microbiota-derived SCFA production	2712:2745	CONCLUSIONS These results demonstrated that YN could ameliorate LPS-induced inflammation in rats by modifying gut microbiota, increasing microbiota-derived SCFA production and regulating the balance of Th1/Th2 and Treg/Th17 cells.
34634365	3	22	theme	microecosystem	525:538	arg1	homeostasis					563:573	the gut microecosystem and CD4+ T cell immune homeostasis	517:573	the gut microecosystem and CD4+ T cell immune homeostasis	517:573	AIMS OF THE STUDY To reveal the effect of YN on gut microbiota dysbiosis, and explore the potential role of the gut microecosystem and CD4+ T cell immune homeostasis in YN-treated respiratory and intestinal diseases in lipopolysaccharide (LPS)-induced inflammatory rats.
34634365	5	23	theme	&	814:814	arg1	staining					818:825	H & E staining	812:825	H & E staining	812:825	Histological changes were observed by H & E staining.
34634365	8	24	theme	Th17	1166:1169	arg1	cells					1180:1184	Th1, Th2, Th17 and Treg cells	1156:1184	Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs)	1156:1221	The frequencies of immune cells, including Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs), bronchoalveolar lavage fluid (BALF) and whole blood were phenotyped using flow cytometry.
34634365	6	25	theme	16S	919:921	arg1	sequencing					933:942	16S rRNA gene sequencing	919:942	16S rRNA gene sequencing	919:942	Changes in gut microbiota and short-chain fatty acid (SCFA) production were analysed using 16S rRNA gene sequencing and targeted metabolomics.
34634365	11	26	from	MLNs	1810:1813	arg1	numbers					1766:1772	The numbers	1762:1772	The numbers of Th17 cells and Treg cells in the MLNs, blood, and BALF of model rats	1762:1844	The numbers of Th17 cells and Treg cells in the MLNs, blood, and BALF of model rats were regulated by YN, with the restoration of the Th17/Treg balance.
34634365	0	27	theme	CD4+	134:137	arg1	cell					141:144	the CD4+ T cell	130:144	the CD4+ T cell balance	130:152	Yanning Syrup ameliorates the lipopolysaccharide-induced inflammation: Adjusting the gut microbiota, short-chain fatty acids, and the CD4+ T cell balance.
34634365	3	28	theme	homeostasis	563:573	arg1	role					509:512	the potential role	495:512	the potential role of the gut microecosystem and CD4+ T cell immune homeostasis in YN-treated respiratory and intestinal diseases in lipopolysaccharide (LPS)-induced inflammatory rats	495:677	AIMS OF THE STUDY To reveal the effect of YN on gut microbiota dysbiosis, and explore the potential role of the gut microecosystem and CD4+ T cell immune homeostasis in YN-treated respiratory and intestinal diseases in lipopolysaccharide (LPS)-induced inflammatory rats.
34634365	7	29	theme	cytokine	981:988	arg1	microarray					990:999	A Luminex cytokine microarray	971:999	A Luminex cytokine microarray	971:999	A Luminex cytokine microarray and enzyme-linked immunosorbent assay (ELISA) were conducted to evaluate the serum and colon cytokine profiles.
34634365	13	30	theme	flora	2130:2134	arg1	composition					2136:2146	flora composition	2130:2146	flora composition	2130:2146	Sequencing of 16S rRNA gene indicated that YN-treated rats exhibited greater gut microbial diversity and flora composition, specifically inhibiting some harmful bacteria such as Enterobacter and Blautia and increasing Firmicutes and Actinobacteria.
34634365	8	31	theme	Treg	1175:1178	arg1	cells					1180:1184	Th1, Th2, Th17 and Treg cells	1156:1184	Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs)	1156:1221	The frequencies of immune cells, including Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs), bronchoalveolar lavage fluid (BALF) and whole blood were phenotyped using flow cytometry.
34634365	11	32	theme	Th17/Treg	1896:1904	arg1	balance					1906:1912	the Th17/Treg balance	1892:1912	the Th17/Treg balance	1892:1912	The numbers of Th17 cells and Treg cells in the MLNs, blood, and BALF of model rats were regulated by YN, with the restoration of the Th17/Treg balance.
34634365	9	33	dep	RESULTS	1314:1320	arg1	rats					1337:1340	The YN-treated rats	1322:1340	RESULTS The YN-treated rats	1314:1340	RESULTS The YN-treated rats showed less colon inflammation, as evidenced by the reduction in mortality rate and histology score.
34634365	11	34	from	Th17 cells	1777:1786	arg1	MLNs					1810:1813	the MLNs	1806:1813	the MLNs	1806:1813	The numbers of Th17 cells and Treg cells in the MLNs, blood, and BALF of model rats were regulated by YN, with the restoration of the Th17/Treg balance.
34634365	0	35	theme	cell	141:144	arg1	balance					146:152	the CD4+ T cell balance	130:152	the CD4+ T cell balance	130:152	Yanning Syrup ameliorates the lipopolysaccharide-induced inflammation: Adjusting the gut microbiota, short-chain fatty acids, and the CD4+ T cell balance.
34634365	3	36	theme	cell	551:554	arg1	homeostasis					563:573	the gut microecosystem and CD4+ T cell immune homeostasis	517:573	the gut microecosystem and CD4+ T cell immune homeostasis	517:573	AIMS OF THE STUDY To reveal the effect of YN on gut microbiota dysbiosis, and explore the potential role of the gut microecosystem and CD4+ T cell immune homeostasis in YN-treated respiratory and intestinal diseases in lipopolysaccharide (LPS)-induced inflammatory rats.
34634365	10	37	theme	IL-12	1723:1727	arg1	levels					1694:1699	the levels	1690:1699	the levels of IL-1α, IL-5, IL-7, IL-12 (p70), GM-CSF and VEGF	1690:1750	Notably, YN was found to improve the immunosuppressed state induced by LPS in rats, which not only upregulated the levels of the proinflammatory cytokine IL-17A and the immunosuppressive cytokines IL-4 and IL-10 in colon tissue but also increased the levels of IL-1α, IL-5, IL-7, IL-12 (p70), GM-CSF and VEGF in serum.
34634365	8	38	theme	lavage	1240:1245	arg1	cells					1139:1143	immune cells	1132:1143	immune cells	1132:1143	The frequencies of immune cells, including Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs), bronchoalveolar lavage fluid (BALF) and whole blood were phenotyped using flow cytometry.
34634365	8	38	theme	lavage	1240:1245	arg1	cells					1180:1184	Th1, Th2, Th17 and Treg cells	1156:1184	Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs)	1156:1221	The frequencies of immune cells, including Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs), bronchoalveolar lavage fluid (BALF) and whole blood were phenotyped using flow cytometry.
34634365	8	38	theme	lavage	1240:1245	arg1	BALF					1254:1257	BALF	1254:1257	BALF	1254:1257	The frequencies of immune cells, including Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs), bronchoalveolar lavage fluid (BALF) and whole blood were phenotyped using flow cytometry.
34634365	8	38	theme	lavage	1240:1245	arg1	fluid					1247:1251	bronchoalveolar lavage fluid	1224:1251	bronchoalveolar lavage fluid (BALF)	1224:1258	The frequencies of immune cells, including Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs), bronchoalveolar lavage fluid (BALF) and whole blood were phenotyped using flow cytometry.
34634365	7	39	theme	Luminex	973:979	arg1	microarray					990:999	A Luminex cytokine microarray	971:999	A Luminex cytokine microarray	971:999	A Luminex cytokine microarray and enzyme-linked immunosorbent assay (ELISA) were conducted to evaluate the serum and colon cytokine profiles.
34634365	9	40	theme	histology	1426:1434	arg1	score					1436:1440	histology score	1426:1440	histology score	1426:1440	RESULTS The YN-treated rats showed less colon inflammation, as evidenced by the reduction in mortality rate and histology score.
34634365	14	41	theme	YN-treated	2413:2422	arg1	rats					2424:2427	YN-treated rats	2413:2427	YN-treated rats	2413:2427	Targeted metabolomics analysis demonstrated an increase of SCFA (acetic acid, butyric acid, valeric acid, and hexanoic acid) production in YN-treated rats.
34634365	16	42	theme	Th1/Th2	2777:2783	arg1	balance					2766:2772	the balance	2762:2772	the balance of Th1/Th2 and Treg/Th17 cells	2762:2803	CONCLUSIONS These results demonstrated that YN could ameliorate LPS-induced inflammation in rats by modifying gut microbiota, increasing microbiota-derived SCFA production and regulating the balance of Th1/Th2 and Treg/Th17 cells.
34634365	3	43	theme	YN-treated	578:587	arg1	diseases					616:623	YN-treated respiratory and intestinal diseases	578:623	YN-treated respiratory and intestinal diseases in lipopolysaccharide (LPS)-induced inflammatory rats	578:677	AIMS OF THE STUDY To reveal the effect of YN on gut microbiota dysbiosis, and explore the potential role of the gut microecosystem and CD4+ T cell immune homeostasis in YN-treated respiratory and intestinal diseases in lipopolysaccharide (LPS)-induced inflammatory rats.
34634365	10	44	theme	immunosuppressed	1480:1495	arg1	state					1497:1501	the immunosuppressed state	1476:1501	the immunosuppressed state induced by LPS in rats, which not only upregulated the levels of the proinflammatory cytokine IL-17A and the immunosuppressive cytokines IL-4 and IL-10 in colon tissue but also increased the levels of IL-1α, IL-5, IL-7, IL-12 (p70), GM-CSF and VEGF in serum	1476:1759	Notably, YN was found to improve the immunosuppressed state induced by LPS in rats, which not only upregulated the levels of the proinflammatory cytokine IL-17A and the immunosuppressive cytokines IL-4 and IL-10 in colon tissue but also increased the levels of IL-1α, IL-5, IL-7, IL-12 (p70), GM-CSF and VEGF in serum.
34634365	13	45	theme	16S	2039:2041	arg1	rRNA					2043:2046	16S rRNA	2039:2046	16S rRNA gene	2039:2051	Sequencing of 16S rRNA gene indicated that YN-treated rats exhibited greater gut microbial diversity and flora composition, specifically inhibiting some harmful bacteria such as Enterobacter and Blautia and increasing Firmicutes and Actinobacteria.
34634365	7	46	theme	enzyme-linked	1005:1017	arg1	ELISA					1040:1044	ELISA	1040:1044	ELISA	1040:1044	A Luminex cytokine microarray and enzyme-linked immunosorbent assay (ELISA) were conducted to evaluate the serum and colon cytokine profiles.
34634365	7	46	theme	enzyme-linked	1005:1017	arg1	assay					1033:1037	enzyme-linked immunosorbent assay	1005:1037	enzyme-linked immunosorbent assay (ELISA)	1005:1045	A Luminex cytokine microarray and enzyme-linked immunosorbent assay (ELISA) were conducted to evaluate the serum and colon cytokine profiles.
34634365	4	47	theme	METHODS	680:686	arg1	Inflammation					688:699	METHODS Inflammation	680:699	METHODS Inflammation in rat models	680:713	METHODS Inflammation in rat models was induced by intraperitoneal injection of LPS (8 mg/kg).
34634365	3	48	from	role	509:512	arg1	diseases					616:623	YN-treated respiratory and intestinal diseases	578:623	YN-treated respiratory and intestinal diseases in lipopolysaccharide (LPS)-induced inflammatory rats	578:677	AIMS OF THE STUDY To reveal the effect of YN on gut microbiota dysbiosis, and explore the potential role of the gut microecosystem and CD4+ T cell immune homeostasis in YN-treated respiratory and intestinal diseases in lipopolysaccharide (LPS)-induced inflammatory rats.
34634365	1	49	theme	ETHNOPHARMACOLOGICAL	155:174	arg1	RELEVANCE					176:184	ETHNOPHARMACOLOGICAL RELEVANCE	155:184	ETHNOPHARMACOLOGICAL RELEVANCE As a commercial Chinese patent medicine, Yanning Syrup (YN)	155:244	ETHNOPHARMACOLOGICAL RELEVANCE As a commercial Chinese patent medicine, Yanning Syrup (YN) is used to treat acute upper respiratory tract infections and acute enteritis effectively in clinical practice.
34634365	8	50	theme	whole	1264:1268	arg1	blood					1270:1274	whole blood	1264:1274	whole blood	1264:1274	The frequencies of immune cells, including Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs), bronchoalveolar lavage fluid (BALF) and whole blood were phenotyped using flow cytometry.
34634365	8	50	theme	whole	1264:1268	arg1	cells					1180:1184	Th1, Th2, Th17 and Treg cells	1156:1184	Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs)	1156:1221	The frequencies of immune cells, including Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs), bronchoalveolar lavage fluid (BALF) and whole blood were phenotyped using flow cytometry.
34634365	8	50	theme	whole	1264:1268	arg1	cells					1139:1143	immune cells	1132:1143	immune cells	1132:1143	The frequencies of immune cells, including Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs), bronchoalveolar lavage fluid (BALF) and whole blood were phenotyped using flow cytometry.
34634365	0	51	theme	short-chain	101:111	arg1	acids					119:123	short-chain fatty acids	101:123	short-chain fatty acids	101:123	Yanning Syrup ameliorates the lipopolysaccharide-induced inflammation: Adjusting the gut microbiota, short-chain fatty acids, and the CD4+ T cell balance.
34634365	12	52	from	balance	1941:1947	arg1	blood					1967:1971	whole blood	1961:1971	whole blood	1961:1971	Additionally, the Th1/Th2 balance in MLNs and whole blood of model rats was restored after YN administration.
34634365	12	52	from	balance	1941:1947	arg1	MLNs					1952:1955	MLNs	1952:1955	MLNs	1952:1955	Additionally, the Th1/Th2 balance in MLNs and whole blood of model rats was restored after YN administration.
34634365	8	53	theme	bronchoalveolar	1224:1238	arg1	cells					1139:1143	immune cells	1132:1143	immune cells	1132:1143	The frequencies of immune cells, including Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs), bronchoalveolar lavage fluid (BALF) and whole blood were phenotyped using flow cytometry.
34634365	8	53	theme	bronchoalveolar	1224:1238	arg1	cells					1180:1184	Th1, Th2, Th17 and Treg cells	1156:1184	Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs)	1156:1221	The frequencies of immune cells, including Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs), bronchoalveolar lavage fluid (BALF) and whole blood were phenotyped using flow cytometry.
34634365	8	53	theme	bronchoalveolar	1224:1238	arg1	BALF					1254:1257	BALF	1254:1257	BALF	1254:1257	The frequencies of immune cells, including Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs), bronchoalveolar lavage fluid (BALF) and whole blood were phenotyped using flow cytometry.
34634365	8	53	theme	bronchoalveolar	1224:1238	arg1	fluid					1247:1251	bronchoalveolar lavage fluid	1224:1251	bronchoalveolar lavage fluid (BALF)	1224:1258	The frequencies of immune cells, including Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs), bronchoalveolar lavage fluid (BALF) and whole blood were phenotyped using flow cytometry.
34634365	10	54	theme	proinflammatory	1572:1586	arg1	cytokine					1588:1595	the proinflammatory cytokine	1568:1595	the proinflammatory cytokine IL-17A	1568:1602	Notably, YN was found to improve the immunosuppressed state induced by LPS in rats, which not only upregulated the levels of the proinflammatory cytokine IL-17A and the immunosuppressive cytokines IL-4 and IL-10 in colon tissue but also increased the levels of IL-1α, IL-5, IL-7, IL-12 (p70), GM-CSF and VEGF in serum.
34634365	15	55	theme	YN	2481:2482	arg1	administration					2484:2497	YN administration	2481:2497	YN administration	2481:2497	Most of the dominant bacterial genera regulated by YN administration were correlated with the concentrations of SCFA and inflammatory cytokines.
34634365	13	56	theme	rRNA	2043:2046	arg1	gene					2048:2051	16S rRNA gene	2039:2051	16S rRNA gene	2039:2051	Sequencing of 16S rRNA gene indicated that YN-treated rats exhibited greater gut microbial diversity and flora composition, specifically inhibiting some harmful bacteria such as Enterobacter and Blautia and increasing Firmicutes and Actinobacteria.
34634365	14	57	from	increase	2321:2328	arg1	rats					2424:2427	YN-treated rats	2413:2427	YN-treated rats	2413:2427	Targeted metabolomics analysis demonstrated an increase of SCFA (acetic acid, butyric acid, valeric acid, and hexanoic acid) production in YN-treated rats.
34634365	12	58	theme	YN	2006:2007	arg1	administration					2009:2022	YN administration	2006:2022	YN administration	2006:2022	Additionally, the Th1/Th2 balance in MLNs and whole blood of model rats was restored after YN administration.
34634365	2	59	theme	underlying	371:380	arg1	mechanism					382:390	the underlying mechanism	367:390	the underlying mechanism	367:390	However, the underlying mechanism remains unclear.
34634365	0	60	theme	Yanning	0:6	arg1	Syrup					8:12	Yanning Syrup	0:12	Yanning Syrup	0:12	Yanning Syrup ameliorates the lipopolysaccharide-induced inflammation: Adjusting the gut microbiota, short-chain fatty acids, and the CD4+ T cell balance.
34634365	4	61	theme	intraperitoneal	730:744	arg1	injection					746:754	intraperitoneal injection	730:754	intraperitoneal injection of LPS (8 mg/kg)	730:771	METHODS Inflammation in rat models was induced by intraperitoneal injection of LPS (8 mg/kg).
34634365	10	62	theme	cytokine	1588:1595	arg1	IL-17A					1597:1602	the proinflammatory cytokine IL-17A	1568:1602	the proinflammatory cytokine IL-17A	1568:1602	Notably, YN was found to improve the immunosuppressed state induced by LPS in rats, which not only upregulated the levels of the proinflammatory cytokine IL-17A and the immunosuppressive cytokines IL-4 and IL-10 in colon tissue but also increased the levels of IL-1α, IL-5, IL-7, IL-12 (p70), GM-CSF and VEGF in serum.
34634365	1	63	theme	upper	269:273	arg1	infections					293:302	acute upper respiratory tract infections	263:302	acute upper respiratory tract infections	263:302	ETHNOPHARMACOLOGICAL RELEVANCE As a commercial Chinese patent medicine, Yanning Syrup (YN) is used to treat acute upper respiratory tract infections and acute enteritis effectively in clinical practice.
34634365	10	64	theme	colon	1658:1662	arg1	tissue					1664:1669	colon tissue	1658:1669	colon tissue	1658:1669	Notably, YN was found to improve the immunosuppressed state induced by LPS in rats, which not only upregulated the levels of the proinflammatory cytokine IL-17A and the immunosuppressive cytokines IL-4 and IL-10 in colon tissue but also increased the levels of IL-1α, IL-5, IL-7, IL-12 (p70), GM-CSF and VEGF in serum.
34634365	6	65	theme	gut	839:841	arg1	microbiota					843:852	gut microbiota	839:852	gut microbiota	839:852	Changes in gut microbiota and short-chain fatty acid (SCFA) production were analysed using 16S rRNA gene sequencing and targeted metabolomics.
34634365	12	66	theme	rats	1982:1985	arg1	blood					1967:1971	whole blood	1961:1971	whole blood	1961:1971	Additionally, the Th1/Th2 balance in MLNs and whole blood of model rats was restored after YN administration.
34634365	12	66	theme	rats	1982:1985	arg1	MLNs					1952:1955	MLNs	1952:1955	MLNs	1952:1955	Additionally, the Th1/Th2 balance in MLNs and whole blood of model rats was restored after YN administration.
34634365	0	67	theme	lipopolysaccharide-induced	30:55	arg1	inflammation					57:68	the lipopolysaccharide-induced inflammation	26:68	the lipopolysaccharide-induced inflammation	26:68	Yanning Syrup ameliorates the lipopolysaccharide-induced inflammation: Adjusting the gut microbiota, short-chain fatty acids, and the CD4+ T cell balance.
34634365	1	68	theme	tract	287:291	arg1	infections					293:302	acute upper respiratory tract infections	263:302	acute upper respiratory tract infections	263:302	ETHNOPHARMACOLOGICAL RELEVANCE As a commercial Chinese patent medicine, Yanning Syrup (YN) is used to treat acute upper respiratory tract infections and acute enteritis effectively in clinical practice.
34634365	15	69	theme	inflammatory	2551:2562	arg1	cytokines					2564:2572	SCFA and inflammatory cytokines	2542:2572	SCFA and inflammatory cytokines	2542:2572	Most of the dominant bacterial genera regulated by YN administration were correlated with the concentrations of SCFA and inflammatory cytokines.
34634365	14	70	theme	valeric	2366:2372	arg1	acid					2374:2377	valeric acid	2366:2377	valeric acid	2366:2377	Targeted metabolomics analysis demonstrated an increase of SCFA (acetic acid, butyric acid, valeric acid, and hexanoic acid) production in YN-treated rats.
34634365	9	71	from	reduction	1394:1402	arg1	rate					1417:1420	mortality rate	1407:1420	mortality rate	1407:1420	RESULTS The YN-treated rats showed less colon inflammation, as evidenced by the reduction in mortality rate and histology score.
34634365	9	71	from	reduction	1394:1402	arg1	score					1436:1440	histology score	1426:1440	histology score	1426:1440	RESULTS The YN-treated rats showed less colon inflammation, as evidenced by the reduction in mortality rate and histology score.
34634365	15	72	theme	SCFA	2542:2545	arg1	cytokines					2564:2572	SCFA and inflammatory cytokines	2542:2572	SCFA and inflammatory cytokines	2542:2572	Most of the dominant bacterial genera regulated by YN administration were correlated with the concentrations of SCFA and inflammatory cytokines.
34634365	3	73	from	effect	441:446	arg1	dysbiosis					472:480	gut microbiota dysbiosis	457:480	gut microbiota dysbiosis	457:480	AIMS OF THE STUDY To reveal the effect of YN on gut microbiota dysbiosis, and explore the potential role of the gut microecosystem and CD4+ T cell immune homeostasis in YN-treated respiratory and intestinal diseases in lipopolysaccharide (LPS)-induced inflammatory rats.
34634365	3	74	theme	microbiota	461:470	arg1	dysbiosis					472:480	gut microbiota dysbiosis	457:480	gut microbiota dysbiosis	457:480	AIMS OF THE STUDY To reveal the effect of YN on gut microbiota dysbiosis, and explore the potential role of the gut microecosystem and CD4+ T cell immune homeostasis in YN-treated respiratory and intestinal diseases in lipopolysaccharide (LPS)-induced inflammatory rats.
34634365	5	75	theme	Histological	774:785	arg1	changes					787:793	Histological changes	774:793	Histological changes	774:793	Histological changes were observed by H & E staining.
34634365	11	76	theme	blood	1816:1820	arg1	numbers					1766:1772	The numbers	1762:1772	The numbers of Th17 cells and Treg cells in the MLNs, blood, and BALF of model rats	1762:1844	The numbers of Th17 cells and Treg cells in the MLNs, blood, and BALF of model rats were regulated by YN, with the restoration of the Th17/Treg balance.
34634365	3	77	theme	-induced	652:659	arg1	rats					674:677	lipopolysaccharide (LPS)-induced inflammatory rats	628:677	lipopolysaccharide (LPS)-induced inflammatory rats	628:677	AIMS OF THE STUDY To reveal the effect of YN on gut microbiota dysbiosis, and explore the potential role of the gut microecosystem and CD4+ T cell immune homeostasis in YN-treated respiratory and intestinal diseases in lipopolysaccharide (LPS)-induced inflammatory rats.
34634365	12	78	theme	whole	1961:1965	arg1	blood					1967:1971	whole blood	1961:1971	whole blood	1961:1971	Additionally, the Th1/Th2 balance in MLNs and whole blood of model rats was restored after YN administration.
34634365	13	79	theme	harmful	2178:2184	arg1	bacteria					2186:2193	some harmful bacteria	2173:2193	some harmful bacteria such as Enterobacter and Blautia	2173:2226	Sequencing of 16S rRNA gene indicated that YN-treated rats exhibited greater gut microbial diversity and flora composition, specifically inhibiting some harmful bacteria such as Enterobacter and Blautia and increasing Firmicutes and Actinobacteria.
34634365	13	79	theme	harmful	2178:2184	arg1	Enterobacter					2203:2214	Enterobacter	2203:2214	Enterobacter	2203:2214	Sequencing of 16S rRNA gene indicated that YN-treated rats exhibited greater gut microbial diversity and flora composition, specifically inhibiting some harmful bacteria such as Enterobacter and Blautia and increasing Firmicutes and Actinobacteria.
34634365	13	79	theme	harmful	2178:2184	arg1	Blautia					2220:2226	Blautia	2220:2226	Blautia	2220:2226	Sequencing of 16S rRNA gene indicated that YN-treated rats exhibited greater gut microbial diversity and flora composition, specifically inhibiting some harmful bacteria such as Enterobacter and Blautia and increasing Firmicutes and Actinobacteria.
34634365	10	80	theme	GM-CSF	1736:1741	arg1	levels					1694:1699	the levels	1690:1699	the levels of IL-1α, IL-5, IL-7, IL-12 (p70), GM-CSF and VEGF	1690:1750	Notably, YN was found to improve the immunosuppressed state induced by LPS in rats, which not only upregulated the levels of the proinflammatory cytokine IL-17A and the immunosuppressive cytokines IL-4 and IL-10 in colon tissue but also increased the levels of IL-1α, IL-5, IL-7, IL-12 (p70), GM-CSF and VEGF in serum.
34634365	4	81	from	Inflammation	688:699	arg1	models					708:713	rat models	704:713	rat models	704:713	METHODS Inflammation in rat models was induced by intraperitoneal injection of LPS (8 mg/kg).
34634365	14	82	theme	acetic	2339:2344	arg1	acid					2346:2349	acetic acid	2339:2349	acetic acid	2339:2349	Targeted metabolomics analysis demonstrated an increase of SCFA (acetic acid, butyric acid, valeric acid, and hexanoic acid) production in YN-treated rats.
34634365	14	83	theme	production	2399:2408	arg1	increase					2321:2328	an increase	2318:2328	an increase of SCFA (acetic acid, butyric acid, valeric acid, and hexanoic acid) production in YN-treated rats	2318:2427	Targeted metabolomics analysis demonstrated an increase of SCFA (acetic acid, butyric acid, valeric acid, and hexanoic acid) production in YN-treated rats.
34634365	11	84	from	BALF	1827:1830	arg1	MLNs					1810:1813	the MLNs	1806:1813	the MLNs	1806:1813	The numbers of Th17 cells and Treg cells in the MLNs, blood, and BALF of model rats were regulated by YN, with the restoration of the Th17/Treg balance.
34634365	7	85	theme	colon	1088:1092	arg1	profiles					1103:1110	the serum and colon cytokine profiles	1074:1110	the serum and colon cytokine profiles	1074:1110	A Luminex cytokine microarray and enzyme-linked immunosorbent assay (ELISA) were conducted to evaluate the serum and colon cytokine profiles.
34634365	14	86	theme	SCFA	2333:2336	arg1	production					2399:2408	SCFA (acetic acid, butyric acid, valeric acid, and hexanoic acid) production	2333:2408	SCFA (acetic acid, butyric acid, valeric acid, and hexanoic acid) production	2333:2408	Targeted metabolomics analysis demonstrated an increase of SCFA (acetic acid, butyric acid, valeric acid, and hexanoic acid) production in YN-treated rats.
34634365	7	87	theme	serum	1078:1082	arg1	profiles					1103:1110	the serum and colon cytokine profiles	1074:1110	the serum and colon cytokine profiles	1074:1110	A Luminex cytokine microarray and enzyme-linked immunosorbent assay (ELISA) were conducted to evaluate the serum and colon cytokine profiles.
34634365	11	88	theme	model	1835:1839	arg1	rats					1841:1844	model rats	1835:1844	model rats	1835:1844	The numbers of Th17 cells and Treg cells in the MLNs, blood, and BALF of model rats were regulated by YN, with the restoration of the Th17/Treg balance.
34634365	11	89	from	numbers	1766:1772	arg1	MLNs					1810:1813	the MLNs	1806:1813	the MLNs	1806:1813	The numbers of Th17 cells and Treg cells in the MLNs, blood, and BALF of model rats were regulated by YN, with the restoration of the Th17/Treg balance.
34634365	6	90	theme	fatty	870:874	arg1	SCFA					882:885	SCFA	882:885	SCFA	882:885	Changes in gut microbiota and short-chain fatty acid (SCFA) production were analysed using 16S rRNA gene sequencing and targeted metabolomics.
34634365	6	90	theme	fatty	870:874	arg1	acid					876:879	short-chain fatty acid	858:879	short-chain fatty acid (SCFA) production	858:897	Changes in gut microbiota and short-chain fatty acid (SCFA) production were analysed using 16S rRNA gene sequencing and targeted metabolomics.
34634365	14	91	theme	Targeted	2274:2281	arg1	analysis					2296:2303	Targeted metabolomics analysis	2274:2303	Targeted metabolomics analysis	2274:2303	Targeted metabolomics analysis demonstrated an increase of SCFA (acetic acid, butyric acid, valeric acid, and hexanoic acid) production in YN-treated rats.
34634365	16	92	link	microbiota-derived	2712:2729	arg1	production					2736:2745	microbiota-derived SCFA production	2712:2745	microbiota-derived SCFA production	2712:2745	CONCLUSIONS These results demonstrated that YN could ameliorate LPS-induced inflammation in rats by modifying gut microbiota, increasing microbiota-derived SCFA production and regulating the balance of Th1/Th2 and Treg/Th17 cells.
34634365	10	93	theme	immunosuppressive	1612:1628	arg1	IL-4					1640:1643	IL-4	1640:1643	IL-4	1640:1643	Notably, YN was found to improve the immunosuppressed state induced by LPS in rats, which not only upregulated the levels of the proinflammatory cytokine IL-17A and the immunosuppressive cytokines IL-4 and IL-10 in colon tissue but also increased the levels of IL-1α, IL-5, IL-7, IL-12 (p70), GM-CSF and VEGF in serum.
34634365	10	93	theme	immunosuppressive	1612:1628	arg1	cytokines					1630:1638	the immunosuppressive cytokines	1608:1638	the immunosuppressive cytokines IL-4 and IL-10	1608:1653	Notably, YN was found to improve the immunosuppressed state induced by LPS in rats, which not only upregulated the levels of the proinflammatory cytokine IL-17A and the immunosuppressive cytokines IL-4 and IL-10 in colon tissue but also increased the levels of IL-1α, IL-5, IL-7, IL-12 (p70), GM-CSF and VEGF in serum.
34634365	10	93	theme	immunosuppressive	1612:1628	arg1	IL-10					1649:1653	IL-10	1649:1653	IL-10	1649:1653	Notably, YN was found to improve the immunosuppressed state induced by LPS in rats, which not only upregulated the levels of the proinflammatory cytokine IL-17A and the immunosuppressive cytokines IL-4 and IL-10 in colon tissue but also increased the levels of IL-1α, IL-5, IL-7, IL-12 (p70), GM-CSF and VEGF in serum.
34634365	3	94	theme	potential	499:507	arg1	role					509:512	the potential role	495:512	the potential role of the gut microecosystem and CD4+ T cell immune homeostasis in YN-treated respiratory and intestinal diseases in lipopolysaccharide (LPS)-induced inflammatory rats	495:677	AIMS OF THE STUDY To reveal the effect of YN on gut microbiota dysbiosis, and explore the potential role of the gut microecosystem and CD4+ T cell immune homeostasis in YN-treated respiratory and intestinal diseases in lipopolysaccharide (LPS)-induced inflammatory rats.
34634365	8	95	theme	cells	1139:1143	arg1	frequencies					1117:1127	The frequencies	1113:1127	The frequencies of immune cells, including Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs), bronchoalveolar lavage fluid (BALF) and whole blood	1113:1274	The frequencies of immune cells, including Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs), bronchoalveolar lavage fluid (BALF) and whole blood were phenotyped using flow cytometry.
34634365	12	96	theme	Th1/Th2	1933:1939	arg1	balance					1941:1947	the Th1/Th2 balance	1929:1947	the Th1/Th2 balance in MLNs and whole blood of model rats	1929:1985	Additionally, the Th1/Th2 balance in MLNs and whole blood of model rats was restored after YN administration.
34634365	6	97	from	Changes	828:834	arg1	microbiota					843:852	gut microbiota	839:852	gut microbiota	839:852	Changes in gut microbiota and short-chain fatty acid (SCFA) production were analysed using 16S rRNA gene sequencing and targeted metabolomics.
34634365	6	97	from	Changes	828:834	arg1	production					888:897	short-chain fatty acid (SCFA) production	858:897	short-chain fatty acid (SCFA) production	858:897	Changes in gut microbiota and short-chain fatty acid (SCFA) production were analysed using 16S rRNA gene sequencing and targeted metabolomics.
34634365	15	98	theme	bacterial	2451:2459	arg1	genera					2461:2466	the dominant bacterial genera	2438:2466	the dominant bacterial genera regulated by YN administration	2438:2497	Most of the dominant bacterial genera regulated by YN administration were correlated with the concentrations of SCFA and inflammatory cytokines.
34634365	16	99	theme	gut	2685:2687	arg1	microbiota					2689:2698	gut microbiota	2685:2698	gut microbiota	2685:2698	CONCLUSIONS These results demonstrated that YN could ameliorate LPS-induced inflammation in rats by modifying gut microbiota, increasing microbiota-derived SCFA production and regulating the balance of Th1/Th2 and Treg/Th17 cells.
34634365	11	100	from	cells	1797:1801	arg1	MLNs					1810:1813	the MLNs	1806:1813	the MLNs	1806:1813	The numbers of Th17 cells and Treg cells in the MLNs, blood, and BALF of model rats were regulated by YN, with the restoration of the Th17/Treg balance.
34634365	16	101	theme	SCFA	2731:2734	arg1	production					2736:2745	microbiota-derived SCFA production	2712:2745	microbiota-derived SCFA production	2712:2745	CONCLUSIONS These results demonstrated that YN could ameliorate LPS-induced inflammation in rats by modifying gut microbiota, increasing microbiota-derived SCFA production and regulating the balance of Th1/Th2 and Treg/Th17 cells.
34634365	3	102	theme	gut	521:523	arg1	microecosystem					525:538	gut microecosystem	521:538	gut microecosystem	521:538	AIMS OF THE STUDY To reveal the effect of YN on gut microbiota dysbiosis, and explore the potential role of the gut microecosystem and CD4+ T cell immune homeostasis in YN-treated respiratory and intestinal diseases in lipopolysaccharide (LPS)-induced inflammatory rats.
34634365	1	103	theme	Chinese	202:208	arg1	medicine					217:224	a commercial Chinese patent medicine	189:224	a commercial Chinese patent medicine	189:224	ETHNOPHARMACOLOGICAL RELEVANCE As a commercial Chinese patent medicine, Yanning Syrup (YN) is used to treat acute upper respiratory tract infections and acute enteritis effectively in clinical practice.
34634365	1	103	theme	Chinese	202:208	arg1	Syrup					235:239	Syrup	235:239	Syrup	235:239	ETHNOPHARMACOLOGICAL RELEVANCE As a commercial Chinese patent medicine, Yanning Syrup (YN) is used to treat acute upper respiratory tract infections and acute enteritis effectively in clinical practice.
34634365	6	104	theme	rRNA	923:926	arg1	sequencing					933:942	16S rRNA gene sequencing	919:942	16S rRNA gene sequencing	919:942	Changes in gut microbiota and short-chain fatty acid (SCFA) production were analysed using 16S rRNA gene sequencing and targeted metabolomics.
34634365	13	105	theme	YN-treated	2068:2077	arg1	rats					2079:2082	YN-treated rats	2068:2082	YN-treated rats	2068:2082	Sequencing of 16S rRNA gene indicated that YN-treated rats exhibited greater gut microbial diversity and flora composition, specifically inhibiting some harmful bacteria such as Enterobacter and Blautia and increasing Firmicutes and Actinobacteria.
34634365	8	106	theme	lymph	1204:1208	arg1	nodes					1210:1214	the mesenteric lymph nodes	1189:1214	the mesenteric lymph nodes (MLNs)	1189:1221	The frequencies of immune cells, including Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs), bronchoalveolar lavage fluid (BALF) and whole blood were phenotyped using flow cytometry.
34634365	8	106	theme	lymph	1204:1208	arg1	MLNs					1217:1220	MLNs	1217:1220	MLNs	1217:1220	The frequencies of immune cells, including Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs), bronchoalveolar lavage fluid (BALF) and whole blood were phenotyped using flow cytometry.
34634365	0	107	theme	T	139:139	arg1	cell					141:144	the CD4+ T cell	130:144	the CD4+ T cell balance	130:152	Yanning Syrup ameliorates the lipopolysaccharide-induced inflammation: Adjusting the gut microbiota, short-chain fatty acids, and the CD4+ T cell balance.
34634365	3	108	theme	immune	556:561	arg1	homeostasis					563:573	the gut microecosystem and CD4+ T cell immune homeostasis	517:573	the gut microecosystem and CD4+ T cell immune homeostasis	517:573	AIMS OF THE STUDY To reveal the effect of YN on gut microbiota dysbiosis, and explore the potential role of the gut microecosystem and CD4+ T cell immune homeostasis in YN-treated respiratory and intestinal diseases in lipopolysaccharide (LPS)-induced inflammatory rats.
34634365	5	109	theme	E	816:816	arg1	staining					818:825	H & E staining	812:825	H & E staining	812:825	Histological changes were observed by H & E staining.
34634365	11	110	theme	BALF	1827:1830	arg1	numbers					1766:1772	The numbers	1762:1772	The numbers of Th17 cells and Treg cells in the MLNs, blood, and BALF of model rats	1762:1844	The numbers of Th17 cells and Treg cells in the MLNs, blood, and BALF of model rats were regulated by YN, with the restoration of the Th17/Treg balance.
34634365	7	111	theme	immunosorbent	1019:1031	arg1	ELISA					1040:1044	ELISA	1040:1044	ELISA	1040:1044	A Luminex cytokine microarray and enzyme-linked immunosorbent assay (ELISA) were conducted to evaluate the serum and colon cytokine profiles.
34634365	7	111	theme	immunosorbent	1019:1031	arg1	assay					1033:1037	enzyme-linked immunosorbent assay	1005:1037	enzyme-linked immunosorbent assay (ELISA)	1005:1045	A Luminex cytokine microarray and enzyme-linked immunosorbent assay (ELISA) were conducted to evaluate the serum and colon cytokine profiles.
34634365	3	112	theme	T	549:549	arg1	cell					551:554	CD4+ T cell	544:554	CD4+ T cell	544:554	AIMS OF THE STUDY To reveal the effect of YN on gut microbiota dysbiosis, and explore the potential role of the gut microecosystem and CD4+ T cell immune homeostasis in YN-treated respiratory and intestinal diseases in lipopolysaccharide (LPS)-induced inflammatory rats.
34634365	9	113	theme	mortality	1407:1415	arg1	rate					1417:1420	mortality rate	1407:1420	mortality rate	1407:1420	RESULTS The YN-treated rats showed less colon inflammation, as evidenced by the reduction in mortality rate and histology score.
34634365	11	114	theme	balance	1906:1912	arg1	restoration					1877:1887	the restoration	1873:1887	the restoration of the Th17/Treg balance	1873:1912	The numbers of Th17 cells and Treg cells in the MLNs, blood, and BALF of model rats were regulated by YN, with the restoration of the Th17/Treg balance.
34634365	10	115	theme	VEGF	1747:1750	arg1	levels					1694:1699	the levels	1690:1699	the levels of IL-1α, IL-5, IL-7, IL-12 (p70), GM-CSF and VEGF	1690:1750	Notably, YN was found to improve the immunosuppressed state induced by LPS in rats, which not only upregulated the levels of the proinflammatory cytokine IL-17A and the immunosuppressive cytokines IL-4 and IL-10 in colon tissue but also increased the levels of IL-1α, IL-5, IL-7, IL-12 (p70), GM-CSF and VEGF in serum.
34634365	3	116	theme	respiratory	589:599	arg1	diseases					616:623	YN-treated respiratory and intestinal diseases	578:623	YN-treated respiratory and intestinal diseases in lipopolysaccharide (LPS)-induced inflammatory rats	578:677	AIMS OF THE STUDY To reveal the effect of YN on gut microbiota dysbiosis, and explore the potential role of the gut microecosystem and CD4+ T cell immune homeostasis in YN-treated respiratory and intestinal diseases in lipopolysaccharide (LPS)-induced inflammatory rats.
34634365	8	117	from	cells	1180:1184	arg1	nodes					1210:1214	the mesenteric lymph nodes	1189:1214	the mesenteric lymph nodes (MLNs)	1189:1221	The frequencies of immune cells, including Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs), bronchoalveolar lavage fluid (BALF) and whole blood were phenotyped using flow cytometry.
34634365	8	117	from	cells	1180:1184	arg1	MLNs					1217:1220	MLNs	1217:1220	MLNs	1217:1220	The frequencies of immune cells, including Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs), bronchoalveolar lavage fluid (BALF) and whole blood were phenotyped using flow cytometry.
34634365	13	118	theme	greater	2094:2100	arg1	diversity					2116:2124	greater gut microbial diversity	2094:2124	greater gut microbial diversity	2094:2124	Sequencing of 16S rRNA gene indicated that YN-treated rats exhibited greater gut microbial diversity and flora composition, specifically inhibiting some harmful bacteria such as Enterobacter and Blautia and increasing Firmicutes and Actinobacteria.
34634365	4	119	theme	rat	704:706	arg1	models					708:713	rat models	704:713	rat models	704:713	METHODS Inflammation in rat models was induced by intraperitoneal injection of LPS (8 mg/kg).
34634365	0	120	theme	gut	85:87	arg1	microbiota					89:98	the gut microbiota	81:98	the gut microbiota	81:98	Yanning Syrup ameliorates the lipopolysaccharide-induced inflammation: Adjusting the gut microbiota, short-chain fatty acids, and the CD4+ T cell balance.
34634365	10	121	theme	IL-7	1717:1720	arg1	levels					1694:1699	the levels	1690:1699	the levels of IL-1α, IL-5, IL-7, IL-12 (p70), GM-CSF and VEGF	1690:1750	Notably, YN was found to improve the immunosuppressed state induced by LPS in rats, which not only upregulated the levels of the proinflammatory cytokine IL-17A and the immunosuppressive cytokines IL-4 and IL-10 in colon tissue but also increased the levels of IL-1α, IL-5, IL-7, IL-12 (p70), GM-CSF and VEGF in serum.
34634365	5	122	theme	H	812:812	arg1	staining					818:825	H & E staining	812:825	H & E staining	812:825	Histological changes were observed by H & E staining.
34634365	13	123	theme	gene	2048:2051	arg1	Sequencing					2025:2034	Sequencing	2025:2034	Sequencing of 16S rRNA gene	2025:2051	Sequencing of 16S rRNA gene indicated that YN-treated rats exhibited greater gut microbial diversity and flora composition, specifically inhibiting some harmful bacteria such as Enterobacter and Blautia and increasing Firmicutes and Actinobacteria.
34634365	14	124	theme	hexanoic	2384:2391	arg1	acid					2393:2396	hexanoic acid	2384:2396	hexanoic acid	2384:2396	Targeted metabolomics analysis demonstrated an increase of SCFA (acetic acid, butyric acid, valeric acid, and hexanoic acid) production in YN-treated rats.
34634365	11	125	theme	Treg	1792:1795	arg1	cells					1797:1801	Treg cells	1792:1801	Treg cells	1792:1801	The numbers of Th17 cells and Treg cells in the MLNs, blood, and BALF of model rats were regulated by YN, with the restoration of the Th17/Treg balance.
34634365	16	126	theme	LPS-induced	2639:2649	arg1	inflammation					2651:2662	LPS-induced inflammation	2639:2662	LPS-induced inflammation in rats	2639:2670	CONCLUSIONS These results demonstrated that YN could ameliorate LPS-induced inflammation in rats by modifying gut microbiota, increasing microbiota-derived SCFA production and regulating the balance of Th1/Th2 and Treg/Th17 cells.
34634365	0	127	theme	fatty	113:117	arg1	acids					119:123	short-chain fatty acids	101:123	short-chain fatty acids	101:123	Yanning Syrup ameliorates the lipopolysaccharide-induced inflammation: Adjusting the gut microbiota, short-chain fatty acids, and the CD4+ T cell balance.
34634365	1	128	theme	clinical	339:346	arg1	practice					348:355	clinical practice	339:355	clinical practice	339:355	ETHNOPHARMACOLOGICAL RELEVANCE As a commercial Chinese patent medicine, Yanning Syrup (YN) is used to treat acute upper respiratory tract infections and acute enteritis effectively in clinical practice.
34634365	13	129	theme	microbial	2106:2114	arg1	diversity					2116:2124	greater gut microbial diversity	2094:2124	greater gut microbial diversity	2094:2124	Sequencing of 16S rRNA gene indicated that YN-treated rats exhibited greater gut microbial diversity and flora composition, specifically inhibiting some harmful bacteria such as Enterobacter and Blautia and increasing Firmicutes and Actinobacteria.
34634365	6	130	theme	targeted	948:955	arg1	metabolomics					957:968	targeted metabolomics	948:968	targeted metabolomics	948:968	Changes in gut microbiota and short-chain fatty acid (SCFA) production were analysed using 16S rRNA gene sequencing and targeted metabolomics.
34634365	14	131	dep	SCFA	2333:2336	arg1	acid					2393:2396	hexanoic acid	2384:2396	hexanoic acid	2384:2396	Targeted metabolomics analysis demonstrated an increase of SCFA (acetic acid, butyric acid, valeric acid, and hexanoic acid) production in YN-treated rats.
34634365	14	131	dep	SCFA	2333:2336	arg1	acid					2346:2349	acetic acid	2339:2349	acetic acid	2339:2349	Targeted metabolomics analysis demonstrated an increase of SCFA (acetic acid, butyric acid, valeric acid, and hexanoic acid) production in YN-treated rats.
34634365	14	131	dep	SCFA	2333:2336	arg1	acid					2360:2363	butyric acid	2352:2363	butyric acid	2352:2363	Targeted metabolomics analysis demonstrated an increase of SCFA (acetic acid, butyric acid, valeric acid, and hexanoic acid) production in YN-treated rats.
34634365	14	131	dep	SCFA	2333:2336	arg1	acid					2374:2377	valeric acid	2366:2377	valeric acid	2366:2377	Targeted metabolomics analysis demonstrated an increase of SCFA (acetic acid, butyric acid, valeric acid, and hexanoic acid) production in YN-treated rats.
34634365	10	132	dep	cytokines	1630:1638	arg1	IL-4					1640:1643	IL-4	1640:1643	IL-4	1640:1643	Notably, YN was found to improve the immunosuppressed state induced by LPS in rats, which not only upregulated the levels of the proinflammatory cytokine IL-17A and the immunosuppressive cytokines IL-4 and IL-10 in colon tissue but also increased the levels of IL-1α, IL-5, IL-7, IL-12 (p70), GM-CSF and VEGF in serum.
34634365	10	132	dep	cytokines	1630:1638	arg1	cytokines					1630:1638	the immunosuppressive cytokines	1608:1638	the immunosuppressive cytokines IL-4 and IL-10	1608:1653	Notably, YN was found to improve the immunosuppressed state induced by LPS in rats, which not only upregulated the levels of the proinflammatory cytokine IL-17A and the immunosuppressive cytokines IL-4 and IL-10 in colon tissue but also increased the levels of IL-1α, IL-5, IL-7, IL-12 (p70), GM-CSF and VEGF in serum.
34634365	10	132	dep	cytokines	1630:1638	arg1	IL-10					1649:1653	IL-10	1649:1653	IL-10	1649:1653	Notably, YN was found to improve the immunosuppressed state induced by LPS in rats, which not only upregulated the levels of the proinflammatory cytokine IL-17A and the immunosuppressive cytokines IL-4 and IL-10 in colon tissue but also increased the levels of IL-1α, IL-5, IL-7, IL-12 (p70), GM-CSF and VEGF in serum.
34634365	10	133	theme	IL-17A	1597:1602	arg1	IL-4					1640:1643	IL-4	1640:1643	IL-4	1640:1643	Notably, YN was found to improve the immunosuppressed state induced by LPS in rats, which not only upregulated the levels of the proinflammatory cytokine IL-17A and the immunosuppressive cytokines IL-4 and IL-10 in colon tissue but also increased the levels of IL-1α, IL-5, IL-7, IL-12 (p70), GM-CSF and VEGF in serum.
34634365	10	133	theme	IL-17A	1597:1602	arg1	levels					1558:1563	the levels	1554:1563	the levels of the proinflammatory cytokine IL-17A	1554:1602	Notably, YN was found to improve the immunosuppressed state induced by LPS in rats, which not only upregulated the levels of the proinflammatory cytokine IL-17A and the immunosuppressive cytokines IL-4 and IL-10 in colon tissue but also increased the levels of IL-1α, IL-5, IL-7, IL-12 (p70), GM-CSF and VEGF in serum.
34634365	10	133	theme	IL-17A	1597:1602	arg1	cytokines					1630:1638	the immunosuppressive cytokines	1608:1638	the immunosuppressive cytokines IL-4 and IL-10	1608:1653	Notably, YN was found to improve the immunosuppressed state induced by LPS in rats, which not only upregulated the levels of the proinflammatory cytokine IL-17A and the immunosuppressive cytokines IL-4 and IL-10 in colon tissue but also increased the levels of IL-1α, IL-5, IL-7, IL-12 (p70), GM-CSF and VEGF in serum.
34634365	10	133	theme	IL-17A	1597:1602	arg1	IL-10					1649:1653	IL-10	1649:1653	IL-10	1649:1653	Notably, YN was found to improve the immunosuppressed state induced by LPS in rats, which not only upregulated the levels of the proinflammatory cytokine IL-17A and the immunosuppressive cytokines IL-4 and IL-10 in colon tissue but also increased the levels of IL-1α, IL-5, IL-7, IL-12 (p70), GM-CSF and VEGF in serum.
34634365	1	134	theme	patent	210:215	arg1	medicine					217:224	a commercial Chinese patent medicine	189:224	a commercial Chinese patent medicine	189:224	ETHNOPHARMACOLOGICAL RELEVANCE As a commercial Chinese patent medicine, Yanning Syrup (YN) is used to treat acute upper respiratory tract infections and acute enteritis effectively in clinical practice.
34634365	1	134	theme	patent	210:215	arg1	Syrup					235:239	Syrup	235:239	Syrup	235:239	ETHNOPHARMACOLOGICAL RELEVANCE As a commercial Chinese patent medicine, Yanning Syrup (YN) is used to treat acute upper respiratory tract infections and acute enteritis effectively in clinical practice.
34634365	4	135	theme	LPS	759:761	arg1	injection					746:754	intraperitoneal injection	730:754	intraperitoneal injection of LPS (8 mg/kg)	730:771	METHODS Inflammation in rat models was induced by intraperitoneal injection of LPS (8 mg/kg).
34634365	3	136	theme	STUDY	421:425	arg1	AIMS					409:412	AIMS	409:412	AIMS OF THE STUDY	409:425	AIMS OF THE STUDY To reveal the effect of YN on gut microbiota dysbiosis, and explore the potential role of the gut microecosystem and CD4+ T cell immune homeostasis in YN-treated respiratory and intestinal diseases in lipopolysaccharide (LPS)-induced inflammatory rats.
34634365	15	137	theme	cytokines	2564:2572	arg1	concentrations					2524:2537	the concentrations	2520:2537	the concentrations of SCFA and inflammatory cytokines	2520:2572	Most of the dominant bacterial genera regulated by YN administration were correlated with the concentrations of SCFA and inflammatory cytokines.
34634365	1	138	theme	acute	263:267	arg1	infections					293:302	acute upper respiratory tract infections	263:302	acute upper respiratory tract infections	263:302	ETHNOPHARMACOLOGICAL RELEVANCE As a commercial Chinese patent medicine, Yanning Syrup (YN) is used to treat acute upper respiratory tract infections and acute enteritis effectively in clinical practice.
34634365	10	139	theme	IL-5	1711:1714	arg1	levels					1694:1699	the levels	1690:1699	the levels of IL-1α, IL-5, IL-7, IL-12 (p70), GM-CSF and VEGF	1690:1750	Notably, YN was found to improve the immunosuppressed state induced by LPS in rats, which not only upregulated the levels of the proinflammatory cytokine IL-17A and the immunosuppressive cytokines IL-4 and IL-10 in colon tissue but also increased the levels of IL-1α, IL-5, IL-7, IL-12 (p70), GM-CSF and VEGF in serum.
34634365	8	140	theme	flow	1298:1301	arg1	cytometry					1303:1311	flow cytometry	1298:1311	flow cytometry	1298:1311	The frequencies of immune cells, including Th1, Th2, Th17 and Treg cells in the mesenteric lymph nodes (MLNs), bronchoalveolar lavage fluid (BALF) and whole blood were phenotyped using flow cytometry.
34634365	3	141	theme	YN	451:452	arg1	effect					441:446	the effect	437:446	the effect of YN on gut microbiota dysbiosis	437:480	AIMS OF THE STUDY To reveal the effect of YN on gut microbiota dysbiosis, and explore the potential role of the gut microecosystem and CD4+ T cell immune homeostasis in YN-treated respiratory and intestinal diseases in lipopolysaccharide (LPS)-induced inflammatory rats.
34634365	1	142	theme	respiratory	275:285	arg1	infections					293:302	acute upper respiratory tract infections	263:302	acute upper respiratory tract infections	263:302	ETHNOPHARMACOLOGICAL RELEVANCE As a commercial Chinese patent medicine, Yanning Syrup (YN) is used to treat acute upper respiratory tract infections and acute enteritis effectively in clinical practice.
34634365	1	143	used	used	249:252	arg2	RELEVANCE					176:184	ETHNOPHARMACOLOGICAL RELEVANCE	155:184	ETHNOPHARMACOLOGICAL RELEVANCE As a commercial Chinese patent medicine, Yanning Syrup (YN)	155:244	ETHNOPHARMACOLOGICAL RELEVANCE As a commercial Chinese patent medicine, Yanning Syrup (YN) is used to treat acute upper respiratory tract infections and acute enteritis effectively in clinical practice.
34634365	13	144	theme	gut	2102:2104	arg1	diversity					2116:2124	greater gut microbial diversity	2094:2124	greater gut microbial diversity	2094:2124	Sequencing of 16S rRNA gene indicated that YN-treated rats exhibited greater gut microbial diversity and flora composition, specifically inhibiting some harmful bacteria such as Enterobacter and Blautia and increasing Firmicutes and Actinobacteria.
34634365	3	145	theme	intestinal	605:614	arg1	diseases					616:623	YN-treated respiratory and intestinal diseases	578:623	YN-treated respiratory and intestinal diseases in lipopolysaccharide (LPS)-induced inflammatory rats	578:677	AIMS OF THE STUDY To reveal the effect of YN on gut microbiota dysbiosis, and explore the potential role of the gut microecosystem and CD4+ T cell immune homeostasis in YN-treated respiratory and intestinal diseases in lipopolysaccharide (LPS)-induced inflammatory rats.
34634365	12	146	theme	model	1976:1980	arg1	rats					1982:1985	model rats	1976:1985	model rats	1976:1985	Additionally, the Th1/Th2 balance in MLNs and whole blood of model rats was restored after YN administration.
34634365	14	147	theme	metabolomics	2283:2294	arg1	analysis					2296:2303	Targeted metabolomics analysis	2274:2303	Targeted metabolomics analysis	2274:2303	Targeted metabolomics analysis demonstrated an increase of SCFA (acetic acid, butyric acid, valeric acid, and hexanoic acid) production in YN-treated rats.
34634365	11	148	theme	Th17 cells	1777:1786	arg1	numbers					1766:1772	The numbers	1762:1772	The numbers of Th17 cells and Treg cells in the MLNs, blood, and BALF of model rats	1762:1844	The numbers of Th17 cells and Treg cells in the MLNs, blood, and BALF of model rats were regulated by YN, with the restoration of the Th17/Treg balance.
34634365	7	149	link	enzyme-linked	1005:1017	arg1	ELISA					1040:1044	ELISA	1040:1044	ELISA	1040:1044	A Luminex cytokine microarray and enzyme-linked immunosorbent assay (ELISA) were conducted to evaluate the serum and colon cytokine profiles.
34634365	7	149	link	enzyme-linked	1005:1017	arg1	assay					1033:1037	enzyme-linked immunosorbent assay	1005:1037	enzyme-linked immunosorbent assay (ELISA)	1005:1045	A Luminex cytokine microarray and enzyme-linked immunosorbent assay (ELISA) were conducted to evaluate the serum and colon cytokine profiles.
34634365	1	150	theme	acute	308:312	arg1	enteritis					314:322	acute enteritis	308:322	acute enteritis	308:322	ETHNOPHARMACOLOGICAL RELEVANCE As a commercial Chinese patent medicine, Yanning Syrup (YN) is used to treat acute upper respiratory tract infections and acute enteritis effectively in clinical practice.
34634365	14	151	theme	butyric	2352:2358	arg1	acid					2360:2363	butyric acid	2352:2363	butyric acid	2352:2363	Targeted metabolomics analysis demonstrated an increase of SCFA (acetic acid, butyric acid, valeric acid, and hexanoic acid) production in YN-treated rats.
34634365	13	152	dep	exhibited	2084:2092	arg1	increasing					2232:2241	increasing	2232:2241	increasing Firmicutes and Actinobacteria	2232:2271	Sequencing of 16S rRNA gene indicated that YN-treated rats exhibited greater gut microbial diversity and flora composition, specifically inhibiting some harmful bacteria such as Enterobacter and Blautia and increasing Firmicutes and Actinobacteria.
34634365	13	152	dep	exhibited	2084:2092	arg1	inhibiting					2162:2171	inhibiting	2162:2171	inhibiting some harmful bacteria such as Enterobacter and Blautia	2162:2226	Sequencing of 16S rRNA gene indicated that YN-treated rats exhibited greater gut microbial diversity and flora composition, specifically inhibiting some harmful bacteria such as Enterobacter and Blautia and increasing Firmicutes and Actinobacteria.
34634365	10	153	dep	only	1537:1540	arg1	not					1533:1535	not	1533:1535	not	1533:1535	Notably, YN was found to improve the immunosuppressed state induced by LPS in rats, which not only upregulated the levels of the proinflammatory cytokine IL-17A and the immunosuppressive cytokines IL-4 and IL-10 in colon tissue but also increased the levels of IL-1α, IL-5, IL-7, IL-12 (p70), GM-CSF and VEGF in serum.
34634365	3	154	theme	gut	457:459	arg1	dysbiosis					472:480	gut microbiota dysbiosis	457:480	gut microbiota dysbiosis	457:480	AIMS OF THE STUDY To reveal the effect of YN on gut microbiota dysbiosis, and explore the potential role of the gut microecosystem and CD4+ T cell immune homeostasis in YN-treated respiratory and intestinal diseases in lipopolysaccharide (LPS)-induced inflammatory rats.
34634365	9	155	theme	YN-treated	1326:1335	arg1	rats					1337:1340	The YN-treated rats	1322:1340	RESULTS The YN-treated rats	1314:1340	RESULTS The YN-treated rats showed less colon inflammation, as evidenced by the reduction in mortality rate and histology score.
33229580	4	0	theme	rapid	837:841	arg1	transformation					857:870	rapid morphological transformation	837:870	rapid morphological transformation from spiral-shaped bacteria to round coccoid cells	837:921	Here, these questions were investigated for a type I toxin-antitoxin system (AapA1-IsoA1) expressed from the chromosome of the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells.
33229580	3	1	theme	biological	515:524	arg1	activity					526:533	the toxins' biological activity	503:533	the toxins' biological activity	503:533	While the regulatory mechanisms of these systems are mostly well defined, the toxins' biological activity and expression conditions are less understood.
33229580	4	2	theme	growth	807:812	arg1	arrest					814:819	growth arrest	807:819	growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells	807:921	Here, these questions were investigated for a type I toxin-antitoxin system (AapA1-IsoA1) expressed from the chromosome of the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells.
33229580	4	3	theme	I	633:633	arg1	AapA1-IsoA1					659:669	AapA1-IsoA1	659:669	AapA1-IsoA1	659:669	Here, these questions were investigated for a type I toxin-antitoxin system (AapA1-IsoA1) expressed from the chromosome of the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells.
33229580	4	3	theme	I	633:633	arg1	system					651:656	a type I toxin-antitoxin system	626:656	a type I toxin-antitoxin system (AapA1-IsoA1) expressed from the chromosome of the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells	626:921	Here, these questions were investigated for a type I toxin-antitoxin system (AapA1-IsoA1) expressed from the chromosome of the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells.
33229580	10	4	theme	Oxidative	1586:1594	arg1	stress					1596:1601	Oxidative stress	1586:1601	Oxidative stress	1586:1601	Oxidative stress induces coccoid formation and is associated with repression of the antitoxin promoter and enhanced processing of its transcript, leading to an imbalance in favor of AapA1 toxin expression.
33229580	10	5	theme	AapA1	1768:1772	arg1	expression					1780:1789	AapA1 toxin expression	1768:1789	AapA1 toxin expression	1768:1789	Oxidative stress induces coccoid formation and is associated with repression of the antitoxin promoter and enhanced processing of its transcript, leading to an imbalance in favor of AapA1 toxin expression.
33229580	1	6	theme	bacterial	175:183	arg1	chromosomes					185:195	many bacterial chromosomes	170:195	many bacterial chromosomes	170:195	Toxin-antitoxin systems are found in many bacterial chromosomes and plasmids with roles ranging from plasmid stabilization to biofilm formation and persistence.
33229580	10	7	from	imbalance	1746:1754	arg1	favor					1759:1763	favor	1759:1763	favor	1759:1763	Oxidative stress induces coccoid formation and is associated with repression of the antitoxin promoter and enhanced processing of its transcript, leading to an imbalance in favor of AapA1 toxin expression.
33229580	11	8	theme	viable	1827:1832	arg1	coccoids					1834:1841	viable coccoids	1827:1841	viable coccoids	1827:1841	Our data support the hypothesis of viable coccoids with characteristics of dormant bacteria that might be important in H. pylori infections refractory to treatment.
33229580	4	9	theme	coccoid	909:915	arg1	cells					917:921	round coccoid cells	903:921	round coccoid cells	903:921	Here, these questions were investigated for a type I toxin-antitoxin system (AapA1-IsoA1) expressed from the chromosome of the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells.
33229580	6	10	theme	pylori	1114:1119	arg1	membrane					1127:1134	H. pylori inner membrane	1111:1134	H. pylori inner membrane	1111:1134	The AapA1 toxin, first molecular effector of coccoids to be identified, targets H. pylori inner membrane without disrupting it, as visualized by cryoelectron microscopy.
33229580	4	11	dep	H.	790:791	arg1	pylori					793:798	H. pylori	790:798	H. pylori	790:798	Here, these questions were investigated for a type I toxin-antitoxin system (AapA1-IsoA1) expressed from the chromosome of the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells.
33229580	11	12	theme	refractory	1932:1941	arg1	infections					1921:1930	H. pylori infections	1911:1930	H. pylori infections refractory to treatment	1911:1954	Our data support the hypothesis of viable coccoids with characteristics of dormant bacteria that might be important in H. pylori infections refractory to treatment.
33229580	4	13	theme	spiral-shaped	877:889	arg1	bacteria					891:898	spiral-shaped bacteria	877:898	spiral-shaped bacteria	877:898	Here, these questions were investigated for a type I toxin-antitoxin system (AapA1-IsoA1) expressed from the chromosome of the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells.
33229580	6	14	theme	cryoelectron	1176:1187	arg1	microscopy					1189:1198	cryoelectron microscopy	1176:1198	cryoelectron microscopy	1176:1198	The AapA1 toxin, first molecular effector of coccoids to be identified, targets H. pylori inner membrane without disrupting it, as visualized by cryoelectron microscopy.
33229580	11	15	dep	H.	1911:1912	arg1	pylori					1914:1919	H. pylori	1911:1919	H. pylori infections refractory to treatment	1911:1954	Our data support the hypothesis of viable coccoids with characteristics of dormant bacteria that might be important in H. pylori infections refractory to treatment.
33229580	1	16	theme	Toxin-antitoxin	133:147	arg1	systems					149:155	Toxin-antitoxin systems	133:155	Toxin-antitoxin systems	133:155	Toxin-antitoxin systems are found in many bacterial chromosomes and plasmids with roles ranging from plasmid stabilization to biofilm formation and persistence.
33229580	10	17	theme	transcript	1720:1729	arg1	repression					1652:1661	repression	1652:1661	repression of the antitoxin promoter	1652:1687	Oxidative stress induces coccoid formation and is associated with repression of the antitoxin promoter and enhanced processing of its transcript, leading to an imbalance in favor of AapA1 toxin expression.
33229580	10	17	theme	transcript	1720:1729	arg1	processing					1702:1711	enhanced processing	1693:1711	enhanced processing of its transcript	1693:1729	Oxidative stress induces coccoid formation and is associated with repression of the antitoxin promoter and enhanced processing of its transcript, leading to an imbalance in favor of AapA1 toxin expression.
33229580	7	18	theme	peptidoglycan	1205:1217	arg1	composition					1219:1229	The peptidoglycan composition	1201:1229	The peptidoglycan composition of coccoids	1201:1241	The peptidoglycan composition of coccoids is modified with respect to spiral bacteria.
33229580	8	19	theme	membrane	1308:1315	arg1	potential					1317:1325	membrane potential	1308:1325	membrane potential	1308:1325	No major changes in membrane potential or adenosine 5'-triphosphate (ATP) concentration result from AapA1 expression, suggesting coccoid viability.
33229580	4	20	dep	pylori	737:742	arg1	show					747:750	show	747:750	show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells	747:921	Here, these questions were investigated for a type I toxin-antitoxin system (AapA1-IsoA1) expressed from the chromosome of the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells.
33229580	2	21	from	RNA	424:426	arg1	systems					399:405	type I systems	392:405	type I systems	392:405	In these systems, the expression/activity of the toxin is counteracted by an antitoxin, which, in type I systems, is an antisense RNA.
33229580	9	22	theme	elongation/division	1552:1570	arg1	interference					1572:1583	cell elongation/division interference	1547:1583	cell elongation/division interference	1547:1583	Single-cell live microscopy tracking the shape conversion suggests a possible association of this process with cell elongation/division interference.
33229580	8	23	theme	5'-triphosphate	1340:1354	arg1	concentration					1362:1374	adenosine 5'-triphosphate (ATP) concentration	1330:1374	adenosine 5'-triphosphate (ATP) concentration	1330:1374	No major changes in membrane potential or adenosine 5'-triphosphate (ATP) concentration result from AapA1 expression, suggesting coccoid viability.
33229580	10	24	theme	enhanced	1693:1700	arg1	processing					1702:1711	enhanced processing	1693:1711	enhanced processing of its transcript	1693:1729	Oxidative stress induces coccoid formation and is associated with repression of the antitoxin promoter and enhanced processing of its transcript, leading to an imbalance in favor of AapA1 toxin expression.
33229580	0	25	theme	spiral	107:112	arg1	shape					114:118	spiral shape	107:118	spiral shape	107:118	A peptide of a type I toxin-antitoxin system induces Helicobacter pylori morphological transformation from spiral shape to coccoids.
33229580	11	26	with	hypothesis	1813:1822	arg1	characteristics					1848:1862	characteristics	1848:1862	characteristics of dormant bacteria that might be important in H. pylori infections refractory to treatment	1848:1954	Our data support the hypothesis of viable coccoids with characteristics of dormant bacteria that might be important in H. pylori infections refractory to treatment.
33229580	5	27	theme	in	969:970	arg1	growth					978:983	in vitro growth	969:983	in vitro growth	969:983	Coccoids are observed in patients and during in vitro growth as a response to different stress conditions.
33229580	6	28	theme	first	1048:1052	arg1	effector					1064:1071	first molecular effector	1048:1071	first molecular effector of coccoids to be identified	1048:1100	The AapA1 toxin, first molecular effector of coccoids to be identified, targets H. pylori inner membrane without disrupting it, as visualized by cryoelectron microscopy.
33229580	6	28	theme	first	1048:1052	arg1	toxin					1041:1045	The AapA1 toxin	1031:1045	The AapA1 toxin	1031:1045	The AapA1 toxin, first molecular effector of coccoids to be identified, targets H. pylori inner membrane without disrupting it, as visualized by cryoelectron microscopy.
33229580	4	29	theme	human	709:713	arg1	pylori					737:742	the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells	705:921	the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells	705:921	Here, these questions were investigated for a type I toxin-antitoxin system (AapA1-IsoA1) expressed from the chromosome of the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells.
33229580	10	30	theme	promoter	1680:1687	arg1	repression					1652:1661	repression	1652:1661	repression of the antitoxin promoter	1652:1687	Oxidative stress induces coccoid formation and is associated with repression of the antitoxin promoter and enhanced processing of its transcript, leading to an imbalance in favor of AapA1 toxin expression.
33229580	10	30	theme	promoter	1680:1687	arg1	processing					1702:1711	enhanced processing	1693:1711	enhanced processing of its transcript	1693:1729	Oxidative stress induces coccoid formation and is associated with repression of the antitoxin promoter and enhanced processing of its transcript, leading to an imbalance in favor of AapA1 toxin expression.
33229580	8	31	from	changes	1297:1303	arg1	potential					1317:1325	membrane potential	1308:1325	membrane potential	1308:1325	No major changes in membrane potential or adenosine 5'-triphosphate (ATP) concentration result from AapA1 expression, suggesting coccoid viability.
33229580	8	31	from	changes	1297:1303	arg1	concentration					1362:1374	adenosine 5'-triphosphate (ATP) concentration	1330:1374	adenosine 5'-triphosphate (ATP) concentration	1330:1374	No major changes in membrane potential or adenosine 5'-triphosphate (ATP) concentration result from AapA1 expression, suggesting coccoid viability.
33229580	2	32	theme	antisense	414:422	arg1	RNA					424:426	an antisense RNA	411:426	an antisense RNA	411:426	In these systems, the expression/activity of the toxin is counteracted by an antitoxin, which, in type I systems, is an antisense RNA.
33229580	2	32	theme	antisense	414:422	arg1	antitoxin					371:379	an antitoxin	368:379	an antitoxin	368:379	In these systems, the expression/activity of the toxin is counteracted by an antitoxin, which, in type I systems, is an antisense RNA.
33229580	4	33	theme	Helicobacter	724:735	arg1	pylori					737:742	the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells	705:921	the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells	705:921	Here, these questions were investigated for a type I toxin-antitoxin system (AapA1-IsoA1) expressed from the chromosome of the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells.
33229580	3	34	theme	regulatory	439:448	arg1	mechanisms					450:459	the regulatory mechanisms	435:459	the regulatory mechanisms of these systems	435:476	While the regulatory mechanisms of these systems are mostly well defined, the toxins' biological activity and expression conditions are less understood.
33229580	0	35	theme	type	15:18	arg1	system					38:43	a type I toxin-antitoxin system	13:43	a type I toxin-antitoxin system	13:43	A peptide of a type I toxin-antitoxin system induces Helicobacter pylori morphological transformation from spiral shape to coccoids.
33229580	3	36	theme	systems	470:476	arg1	mechanisms					450:459	the regulatory mechanisms	435:459	the regulatory mechanisms of these systems	435:476	While the regulatory mechanisms of these systems are mostly well defined, the toxins' biological activity and expression conditions are less understood.
33229580	0	37	theme	toxin-antitoxin	22:36	arg1	system					38:43	a type I toxin-antitoxin system	13:43	a type I toxin-antitoxin system	13:43	A peptide of a type I toxin-antitoxin system induces Helicobacter pylori morphological transformation from spiral shape to coccoids.
33229580	5	38	theme	stress	1012:1017	arg1	conditions					1019:1028	different stress conditions	1002:1028	different stress conditions	1002:1028	Coccoids are observed in patients and during in vitro growth as a response to different stress conditions.
33229580	9	39	dep	microscopy	1453:1462	arg1	tracking					1464:1471	tracking	1464:1471	tracking	1464:1471	Single-cell live microscopy tracking the shape conversion suggests a possible association of this process with cell elongation/division interference.
33229580	2	40	theme	toxin	343:347	arg1	expression/activity					316:334	the expression/activity	312:334	the expression/activity of the toxin	312:347	In these systems, the expression/activity of the toxin is counteracted by an antitoxin, which, in type I systems, is an antisense RNA.
33229580	4	41	theme	AapA1	775:779	arg1	toxin					781:785	the AapA1 toxin	771:785	the AapA1 toxin	771:785	Here, these questions were investigated for a type I toxin-antitoxin system (AapA1-IsoA1) expressed from the chromosome of the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells.
33229580	11	42	theme	H.	1911:1912	arg1	infections					1921:1930	H. pylori infections	1911:1930	H. pylori infections refractory to treatment	1911:1954	Our data support the hypothesis of viable coccoids with characteristics of dormant bacteria that might be important in H. pylori infections refractory to treatment.
33229580	9	43	theme	Single-cell	1436:1446	arg1	microscopy					1453:1462	Single-cell live microscopy	1436:1462	Single-cell live microscopy tracking the shape conversion	1436:1492	Single-cell live microscopy tracking the shape conversion suggests a possible association of this process with cell elongation/division interference.
33229580	6	44	theme	coccoids	1076:1083	arg1	effector					1064:1071	first molecular effector	1048:1071	first molecular effector of coccoids to be identified	1048:1100	The AapA1 toxin, first molecular effector of coccoids to be identified, targets H. pylori inner membrane without disrupting it, as visualized by cryoelectron microscopy.
33229580	6	44	theme	coccoids	1076:1083	arg1	toxin					1041:1045	The AapA1 toxin	1031:1045	The AapA1 toxin	1031:1045	The AapA1 toxin, first molecular effector of coccoids to be identified, targets H. pylori inner membrane without disrupting it, as visualized by cryoelectron microscopy.
33229580	1	45	theme	biofilm	259:265	arg1	formation					267:275	biofilm formation	259:275	biofilm formation	259:275	Toxin-antitoxin systems are found in many bacterial chromosomes and plasmids with roles ranging from plasmid stabilization to biofilm formation and persistence.
33229580	10	46	theme	coccoid	1611:1617	arg1	formation					1619:1627	coccoid formation	1611:1627	coccoid formation	1611:1627	Oxidative stress induces coccoid formation and is associated with repression of the antitoxin promoter and enhanced processing of its transcript, leading to an imbalance in favor of AapA1 toxin expression.
33229580	4	47	theme	morphological	843:855	arg1	transformation					857:870	rapid morphological transformation	837:870	rapid morphological transformation from spiral-shaped bacteria to round coccoid cells	837:921	Here, these questions were investigated for a type I toxin-antitoxin system (AapA1-IsoA1) expressed from the chromosome of the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells.
33229580	10	48	theme	toxin	1774:1778	arg1	expression					1780:1789	AapA1 toxin expression	1768:1789	AapA1 toxin expression	1768:1789	Oxidative stress induces coccoid formation and is associated with repression of the antitoxin promoter and enhanced processing of its transcript, leading to an imbalance in favor of AapA1 toxin expression.
33229580	1	49	theme	many	170:173	arg1	chromosomes					185:195	many bacterial chromosomes	170:195	many bacterial chromosomes	170:195	Toxin-antitoxin systems are found in many bacterial chromosomes and plasmids with roles ranging from plasmid stabilization to biofilm formation and persistence.
33229580	4	50	theme	type	628:631	arg1	AapA1-IsoA1					659:669	AapA1-IsoA1	659:669	AapA1-IsoA1	659:669	Here, these questions were investigated for a type I toxin-antitoxin system (AapA1-IsoA1) expressed from the chromosome of the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells.
33229580	4	50	theme	type	628:631	arg1	system					651:656	a type I toxin-antitoxin system	626:656	a type I toxin-antitoxin system (AapA1-IsoA1) expressed from the chromosome of the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells	626:921	Here, these questions were investigated for a type I toxin-antitoxin system (AapA1-IsoA1) expressed from the chromosome of the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells.
33229580	9	51	theme	live	1448:1451	arg1	microscopy					1453:1462	Single-cell live microscopy	1436:1462	Single-cell live microscopy tracking the shape conversion	1436:1492	Single-cell live microscopy tracking the shape conversion suggests a possible association of this process with cell elongation/division interference.
33229580	6	52	theme	inner	1121:1125	arg1	membrane					1127:1134	H. pylori inner membrane	1111:1134	H. pylori inner membrane	1111:1134	The AapA1 toxin, first molecular effector of coccoids to be identified, targets H. pylori inner membrane without disrupting it, as visualized by cryoelectron microscopy.
33229580	4	53	theme	toxin-antitoxin	635:649	arg1	AapA1-IsoA1					659:669	AapA1-IsoA1	659:669	AapA1-IsoA1	659:669	Here, these questions were investigated for a type I toxin-antitoxin system (AapA1-IsoA1) expressed from the chromosome of the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells.
33229580	4	53	theme	toxin-antitoxin	635:649	arg1	system					651:656	a type I toxin-antitoxin system	626:656	a type I toxin-antitoxin system (AapA1-IsoA1) expressed from the chromosome of the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells	626:921	Here, these questions were investigated for a type I toxin-antitoxin system (AapA1-IsoA1) expressed from the chromosome of the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells.
33229580	11	54	theme	coccoids	1834:1841	arg1	hypothesis					1813:1822	the hypothesis	1809:1822	the hypothesis of viable coccoids with characteristics of dormant bacteria that might be important in H. pylori infections refractory to treatment	1809:1954	Our data support the hypothesis of viable coccoids with characteristics of dormant bacteria that might be important in H. pylori infections refractory to treatment.
33229580	4	55	theme	round	903:907	arg1	cells					917:921	round coccoid cells	903:921	round coccoid cells	903:921	Here, these questions were investigated for a type I toxin-antitoxin system (AapA1-IsoA1) expressed from the chromosome of the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells.
33229580	6	56	theme	H.	1111:1112	arg1	membrane					1127:1134	H. pylori inner membrane	1111:1134	H. pylori inner membrane	1111:1134	The AapA1 toxin, first molecular effector of coccoids to be identified, targets H. pylori inner membrane without disrupting it, as visualized by cryoelectron microscopy.
33229580	9	57	theme	shape	1477:1481	arg1	conversion					1483:1492	the shape conversion	1473:1492	the shape conversion	1473:1492	Single-cell live microscopy tracking the shape conversion suggests a possible association of this process with cell elongation/division interference.
33229580	11	58	theme	dormant	1867:1873	arg1	bacteria					1875:1882	dormant bacteria	1867:1882	dormant bacteria that might be important in H. pylori infections refractory to treatment	1867:1954	Our data support the hypothesis of viable coccoids with characteristics of dormant bacteria that might be important in H. pylori infections refractory to treatment.
33229580	0	59	from	shape	114:118	arg1	transformation					87:100	Helicobacter pylori morphological transformation	53:100	Helicobacter pylori morphological transformation from spiral shape to coccoids	53:130	A peptide of a type I toxin-antitoxin system induces Helicobacter pylori morphological transformation from spiral shape to coccoids.
33229580	5	60	dep	in	969:970	arg1	vitro					972:976	vitro	972:976	vitro	972:976	Coccoids are observed in patients and during in vitro growth as a response to different stress conditions.
33229580	4	61	from	bacteria	891:898	arg1	transformation					857:870	rapid morphological transformation	837:870	rapid morphological transformation from spiral-shaped bacteria to round coccoid cells	837:921	Here, these questions were investigated for a type I toxin-antitoxin system (AapA1-IsoA1) expressed from the chromosome of the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells.
33229580	9	62	theme	possible	1505:1512	arg1	association					1514:1524	a possible association	1503:1524	a possible association of this process with cell elongation/division interference	1503:1583	Single-cell live microscopy tracking the shape conversion suggests a possible association of this process with cell elongation/division interference.
33229580	11	63	from	infections	1921:1930	arg1	important					1898:1906	important	1898:1906	important	1898:1906	Our data support the hypothesis of viable coccoids with characteristics of dormant bacteria that might be important in H. pylori infections refractory to treatment.
33229580	11	64	theme	bacteria	1875:1882	arg1	characteristics					1848:1862	characteristics	1848:1862	characteristics of dormant bacteria that might be important in H. pylori infections refractory to treatment	1848:1954	Our data support the hypothesis of viable coccoids with characteristics of dormant bacteria that might be important in H. pylori infections refractory to treatment.
33229580	0	65	theme	Helicobacter	53:64	arg1	transformation					87:100	Helicobacter pylori morphological transformation	53:100	Helicobacter pylori morphological transformation from spiral shape to coccoids	53:130	A peptide of a type I toxin-antitoxin system induces Helicobacter pylori morphological transformation from spiral shape to coccoids.
33229580	3	66	dep	activity	526:533	arg1	conditions					550:559	conditions	550:559	conditions	550:559	While the regulatory mechanisms of these systems are mostly well defined, the toxins' biological activity and expression conditions are less understood.
33229580	8	67	theme	major	1291:1295	arg1	changes					1297:1303	No major changes	1288:1303	No major changes in membrane potential or adenosine 5'-triphosphate (ATP) concentration	1288:1374	No major changes in membrane potential or adenosine 5'-triphosphate (ATP) concentration result from AapA1 expression, suggesting coccoid viability.
33229580	0	68	theme	morphological	73:85	arg1	transformation					87:100	Helicobacter pylori morphological transformation	53:100	Helicobacter pylori morphological transformation from spiral shape to coccoids	53:130	A peptide of a type I toxin-antitoxin system induces Helicobacter pylori morphological transformation from spiral shape to coccoids.
33229580	4	69	from	expression	757:766	arg1	H.					790:791	H.	790:791	H.	790:791	Here, these questions were investigated for a type I toxin-antitoxin system (AapA1-IsoA1) expressed from the chromosome of the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells.
33229580	9	70	theme	process	1534:1540	arg1	association					1514:1524	a possible association	1503:1524	a possible association of this process with cell elongation/division interference	1503:1583	Single-cell live microscopy tracking the shape conversion suggests a possible association of this process with cell elongation/division interference.
33229580	11	71	from	important	1898:1906	arg1	infections					1921:1930	H. pylori infections	1911:1930	H. pylori infections refractory to treatment	1911:1954	Our data support the hypothesis of viable coccoids with characteristics of dormant bacteria that might be important in H. pylori infections refractory to treatment.
33229580	9	72	theme	cell	1547:1550	arg1	interference					1572:1583	cell elongation/division interference	1547:1583	cell elongation/division interference	1547:1583	Single-cell live microscopy tracking the shape conversion suggests a possible association of this process with cell elongation/division interference.
33229580	4	73	theme	pylori	737:742	arg1	chromosome					691:700	the chromosome	687:700	the chromosome of the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells	687:921	Here, these questions were investigated for a type I toxin-antitoxin system (AapA1-IsoA1) expressed from the chromosome of the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells.
33229580	6	74	theme	molecular	1054:1062	arg1	effector					1064:1071	first molecular effector	1048:1071	first molecular effector of coccoids to be identified	1048:1100	The AapA1 toxin, first molecular effector of coccoids to be identified, targets H. pylori inner membrane without disrupting it, as visualized by cryoelectron microscopy.
33229580	6	74	theme	molecular	1054:1062	arg1	toxin					1041:1045	The AapA1 toxin	1031:1045	The AapA1 toxin	1031:1045	The AapA1 toxin, first molecular effector of coccoids to be identified, targets H. pylori inner membrane without disrupting it, as visualized by cryoelectron microscopy.
33229580	9	75	with	association	1514:1524	arg1	interference					1572:1583	cell elongation/division interference	1547:1583	cell elongation/division interference	1547:1583	Single-cell live microscopy tracking the shape conversion suggests a possible association of this process with cell elongation/division interference.
33229580	8	76	theme	adenosine	1330:1338	arg1	ATP					1357:1359	ATP	1357:1359	ATP	1357:1359	No major changes in membrane potential or adenosine 5'-triphosphate (ATP) concentration result from AapA1 expression, suggesting coccoid viability.
33229580	8	76	theme	adenosine	1330:1338	arg1	5'-triphosphate					1340:1354	adenosine 5'-triphosphate	1330:1354	adenosine 5'-triphosphate (ATP) concentration	1330:1374	No major changes in membrane potential or adenosine 5'-triphosphate (ATP) concentration result from AapA1 expression, suggesting coccoid viability.
33229580	1	77	located	found	161:165	arg1	chromosomes					185:195	many bacterial chromosomes	170:195	many bacterial chromosomes	170:195	Toxin-antitoxin systems are found in many bacterial chromosomes and plasmids with roles ranging from plasmid stabilization to biofilm formation and persistence.
33229580	1	77	located	found	161:165	arg2	systems					149:155	Toxin-antitoxin systems	133:155	Toxin-antitoxin systems	133:155	Toxin-antitoxin systems are found in many bacterial chromosomes and plasmids with roles ranging from plasmid stabilization to biofilm formation and persistence.
33229580	1	77	located	found	161:165	arg1	plasmids					201:208	plasmids	201:208	plasmids with roles ranging from plasmid stabilization to biofilm formation and persistence	201:291	Toxin-antitoxin systems are found in many bacterial chromosomes and plasmids with roles ranging from plasmid stabilization to biofilm formation and persistence.
33229580	10	78	theme	antitoxin	1670:1678	arg1	promoter					1680:1687	the antitoxin promoter	1666:1687	the antitoxin promoter	1666:1687	Oxidative stress induces coccoid formation and is associated with repression of the antitoxin promoter and enhanced processing of its transcript, leading to an imbalance in favor of AapA1 toxin expression.
33229580	0	79	dep	Helicobacter	53:64	arg1	pylori					66:71	pylori	66:71	pylori	66:71	A peptide of a type I toxin-antitoxin system induces Helicobacter pylori morphological transformation from spiral shape to coccoids.
33229580	6	80	theme	AapA1	1035:1039	arg1	effector					1064:1071	first molecular effector	1048:1071	first molecular effector of coccoids to be identified	1048:1100	The AapA1 toxin, first molecular effector of coccoids to be identified, targets H. pylori inner membrane without disrupting it, as visualized by cryoelectron microscopy.
33229580	6	80	theme	AapA1	1035:1039	arg1	toxin					1041:1045	The AapA1 toxin	1031:1045	The AapA1 toxin	1031:1045	The AapA1 toxin, first molecular effector of coccoids to be identified, targets H. pylori inner membrane without disrupting it, as visualized by cryoelectron microscopy.
33229580	4	81	theme	pathogen	715:722	arg1	pylori					737:742	the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells	705:921	the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells	705:921	Here, these questions were investigated for a type I toxin-antitoxin system (AapA1-IsoA1) expressed from the chromosome of the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells.
33229580	0	82	theme	I	20:20	arg1	system					38:43	a type I toxin-antitoxin system	13:43	a type I toxin-antitoxin system	13:43	A peptide of a type I toxin-antitoxin system induces Helicobacter pylori morphological transformation from spiral shape to coccoids.
33229580	1	83	with	plasmids	201:208	arg1	roles					215:219	roles	215:219	roles ranging from plasmid stabilization to biofilm formation and persistence	215:291	Toxin-antitoxin systems are found in many bacterial chromosomes and plasmids with roles ranging from plasmid stabilization to biofilm formation and persistence.
33229580	7	84	theme	spiral	1271:1276	arg1	bacteria					1278:1285	spiral bacteria	1271:1285	spiral bacteria	1271:1285	The peptidoglycan composition of coccoids is modified with respect to spiral bacteria.
33229580	2	85	theme	type	392:395	arg1	systems					399:405	type I systems	392:405	type I systems	392:405	In these systems, the expression/activity of the toxin is counteracted by an antitoxin, which, in type I systems, is an antisense RNA.
33229580	4	86	theme	toxin	781:785	arg1	expression					757:766	expression	757:766	expression of the AapA1 toxin in H. pylori	757:798	Here, these questions were investigated for a type I toxin-antitoxin system (AapA1-IsoA1) expressed from the chromosome of the human pathogen Helicobacter pylori We show that expression of the AapA1 toxin in H. pylori causes growth arrest associated with rapid morphological transformation from spiral-shaped bacteria to round coccoid cells.
33229580	2	87	theme	I	397:397	arg1	systems					399:405	type I systems	392:405	type I systems	392:405	In these systems, the expression/activity of the toxin is counteracted by an antitoxin, which, in type I systems, is an antisense RNA.
33229580	0	88	theme	system	38:43	arg1	peptide					2:8	A peptide	0:8	A peptide of a type I toxin-antitoxin system	0:43	A peptide of a type I toxin-antitoxin system induces Helicobacter pylori morphological transformation from spiral shape to coccoids.
33229580	1	89	with	chromosomes	185:195	arg1	roles					215:219	roles	215:219	roles ranging from plasmid stabilization to biofilm formation and persistence	215:291	Toxin-antitoxin systems are found in many bacterial chromosomes and plasmids with roles ranging from plasmid stabilization to biofilm formation and persistence.
33229580	5	90	theme	different	1002:1010	arg1	conditions					1019:1028	different stress conditions	1002:1028	different stress conditions	1002:1028	Coccoids are observed in patients and during in vitro growth as a response to different stress conditions.
33229580	8	91	theme	AapA1	1388:1392	arg1	expression					1394:1403	AapA1 expression	1388:1403	AapA1 expression	1388:1403	No major changes in membrane potential or adenosine 5'-triphosphate (ATP) concentration result from AapA1 expression, suggesting coccoid viability.
33229580	5	92	located	observed	937:944	arg2	Coccoids					924:931	Coccoids	924:931	Coccoids	924:931	Coccoids are observed in patients and during in vitro growth as a response to different stress conditions.
33229580	5	92	located	observed	937:944	arg2	response					990:997	a response	988:997	a response to different stress conditions	988:1028	Coccoids are observed in patients and during in vitro growth as a response to different stress conditions.
33229580	5	92	located	observed	937:944	arg1	patients					949:956	patients	949:956	patients	949:956	Coccoids are observed in patients and during in vitro growth as a response to different stress conditions.
33229580	1	93	theme	plasmid	234:240	arg1	stabilization					242:254	plasmid stabilization	234:254	plasmid stabilization	234:254	Toxin-antitoxin systems are found in many bacterial chromosomes and plasmids with roles ranging from plasmid stabilization to biofilm formation and persistence.
33229580	7	94	theme	coccoids	1234:1241	arg1	composition					1219:1229	The peptidoglycan composition	1201:1229	The peptidoglycan composition of coccoids	1201:1241	The peptidoglycan composition of coccoids is modified with respect to spiral bacteria.
33229580	8	95	theme	coccoid	1417:1423	arg1	viability					1425:1433	coccoid viability	1417:1433	coccoid viability	1417:1433	No major changes in membrane potential or adenosine 5'-triphosphate (ATP) concentration result from AapA1 expression, suggesting coccoid viability.
33909548	3	0	dep	optimum	405:411	arg1	37 °C					414:418	37 °C	414:418	37 °C	414:418	Strain CMS6Z-2T grew at 10-37 °C (optimum, 37 °C), pH 6.0-9.0 (optimum, pH 7.0-8.0) and in the presence of 0-10.0 % (w/v) NaCl (optimum, 0-1.0 %).
33909548	11	1	theme	phenotypic	1597:1606	arg1	characteristics					1627:1641	phylogenetic, phenotypic and chemotaxonomic characteristics	1583:1641	phylogenetic, phenotypic and chemotaxonomic characteristics	1583:1641	On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain CMS6Z-2T represents a novel species of the genus Phycicoccus, for which the name Phycicoccus flavus sp.
33909548	3	2	theme	w/v	488:490	arg1	NaCl					493:496	0-10.0 % (w/v) NaCl	478:496	0-10.0 % (w/v) NaCl (optimum, 0-1.0 %)	478:515	Strain CMS6Z-2T grew at 10-37 °C (optimum, 37 °C), pH 6.0-9.0 (optimum, pH 7.0-8.0) and in the presence of 0-10.0 % (w/v) NaCl (optimum, 0-1.0 %).
33909548	3	2	theme	w/v	488:490	arg1	optimum					499:505	optimum	499:505	optimum	499:505	Strain CMS6Z-2T grew at 10-37 °C (optimum, 37 °C), pH 6.0-9.0 (optimum, pH 7.0-8.0) and in the presence of 0-10.0 % (w/v) NaCl (optimum, 0-1.0 %).
33909548	11	3	theme	phylogenetic	1583:1594	arg1	characteristics					1627:1641	phylogenetic, phenotypic and chemotaxonomic characteristics	1583:1641	phylogenetic, phenotypic and chemotaxonomic characteristics	1583:1641	On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain CMS6Z-2T represents a novel species of the genus Phycicoccus, for which the name Phycicoccus flavus sp.
33909548	9	4	theme	Phylogenetic	1097:1108	arg1	analyses					1110:1117	Phylogenetic analyses	1097:1117	Phylogenetic analyses based on 16S rRNA gene sequence and phylogenomic analysis based on core proteomes alignment	1097:1209	Phylogenetic analyses based on 16S rRNA gene sequence and phylogenomic analysis based on core proteomes alignment revealed that strain CMS6Z-2T belonged to the genus Phycicoccus and formed a robust cluster with Phycicoccus endophyticus IP6SC6T within the genus Phycicoccus.
33909548	3	5	theme	%	485:485	arg1	NaCl					493:496	0-10.0 % (w/v) NaCl	478:496	0-10.0 % (w/v) NaCl (optimum, 0-1.0 %)	478:515	Strain CMS6Z-2T grew at 10-37 °C (optimum, 37 °C), pH 6.0-9.0 (optimum, pH 7.0-8.0) and in the presence of 0-10.0 % (w/v) NaCl (optimum, 0-1.0 %).
33909548	3	5	theme	%	485:485	arg1	optimum					499:505	optimum	499:505	optimum	499:505	Strain CMS6Z-2T grew at 10-37 °C (optimum, 37 °C), pH 6.0-9.0 (optimum, pH 7.0-8.0) and in the presence of 0-10.0 % (w/v) NaCl (optimum, 0-1.0 %).
33909548	8	6	theme	%	1083:1083	arg1	similarity					1085:1094	98.3 % similarity	1078:1094	98.3 % similarity	1078:1094	Comparative analysis of 16S rRNA genes showed that strain CMS6Z-2T should be assigned to the genus Phycicoccus and its closest relative was Phycicoccus endophyticus IP6SC6T with 98.3 % similarity.
33909548	11	7	theme	Phycicoccus	1732:1742	arg1	sp					1751:1752	the name Phycicoccus flavus sp	1723:1752	the name Phycicoccus flavus sp	1723:1752	On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain CMS6Z-2T represents a novel species of the genus Phycicoccus, for which the name Phycicoccus flavus sp.
33909548	8	8	theme	Comparative	900:910	arg1	analysis					912:919	Comparative analysis	900:919	Comparative analysis of 16S rRNA genes	900:937	Comparative analysis of 16S rRNA genes showed that strain CMS6Z-2T should be assigned to the genus Phycicoccus and its closest relative was Phycicoccus endophyticus IP6SC6T with 98.3 % similarity.
33909548	9	9	theme	16S	1128:1130	arg1	rRNA					1132:1135	16S rRNA	1128:1135	16S rRNA gene sequence	1128:1149	Phylogenetic analyses based on 16S rRNA gene sequence and phylogenomic analysis based on core proteomes alignment revealed that strain CMS6Z-2T belonged to the genus Phycicoccus and formed a robust cluster with Phycicoccus endophyticus IP6SC6T within the genus Phycicoccus.
33909548	3	10	theme	NaCl	493:496	arg1	presence					466:473	the presence	462:473	the presence of 0-10.0 % (w/v) NaCl (optimum, 0-1.0 %)	462:515	Strain CMS6Z-2T grew at 10-37 °C (optimum, 37 °C), pH 6.0-9.0 (optimum, pH 7.0-8.0) and in the presence of 0-10.0 % (w/v) NaCl (optimum, 0-1.0 %).
33909548	9	11	dep	Phycicoccus	1308:1318	arg1	endophyticus					1320:1331	endophyticus	1320:1331	endophyticus	1320:1331	Phylogenetic analyses based on 16S rRNA gene sequence and phylogenomic analysis based on core proteomes alignment revealed that strain CMS6Z-2T belonged to the genus Phycicoccus and formed a robust cluster with Phycicoccus endophyticus IP6SC6T within the genus Phycicoccus.
33909548	9	12	theme	gene	1137:1140	arg1	sequence					1142:1149	16S rRNA gene sequence	1128:1149	16S rRNA gene sequence	1128:1149	Phylogenetic analyses based on 16S rRNA gene sequence and phylogenomic analysis based on core proteomes alignment revealed that strain CMS6Z-2T belonged to the genus Phycicoccus and formed a robust cluster with Phycicoccus endophyticus IP6SC6T within the genus Phycicoccus.
33909548	6	13	theme	unknown	827:833	arg1	phospholipids					835:847	two unknown phospholipids	823:847	two unknown phospholipids	823:847	The polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and two unknown phospholipids.
33909548	9	14	theme	analysis	1168:1175	arg1	alignment					1201:1209	16S rRNA gene sequence and phylogenomic analysis based on core proteomes alignment	1128:1209	16S rRNA gene sequence and phylogenomic analysis based on core proteomes alignment	1128:1209	Phylogenetic analyses based on 16S rRNA gene sequence and phylogenomic analysis based on core proteomes alignment revealed that strain CMS6Z-2T belonged to the genus Phycicoccus and formed a robust cluster with Phycicoccus endophyticus IP6SC6T within the genus Phycicoccus.
33909548	4	15	contain	possessed	534:542	arg2	acid					584:587	the diamino acid	572:587	the diamino acid of the peptidoglycan and MK-8 (H4) as the predominant menaquinone	572:653	Strain CMS6Z-2T possessed meso-diaminopimelic acid as the diamino acid of the peptidoglycan and MK-8 (H4) as the predominant menaquinone.
33909548	4	15	contain	possessed	534:542	arg1	CMS6Z-2T					525:532	Strain CMS6Z-2T	518:532	Strain CMS6Z-2T	518:532	Strain CMS6Z-2T possessed meso-diaminopimelic acid as the diamino acid of the peptidoglycan and MK-8 (H4) as the predominant menaquinone.
33909548	4	15	contain	possessed	534:542	arg2	acid					564:567	meso-diaminopimelic acid	544:567	meso-diaminopimelic acid	544:567	Strain CMS6Z-2T possessed meso-diaminopimelic acid as the diamino acid of the peptidoglycan and MK-8 (H4) as the predominant menaquinone.
33909548	2	16	dep	candel	289:294	arg1	Region					353:358	Autonomous Region	342:358	Autonomous Region	342:358	A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium, designated strain CMS6Z-2T, was isolated from a surface-sterilized branch of Kandelia candel collected from the Maowei Sea, Guangxi Zhuang Autonomous Region, PR China.
33909548	2	16	dep	candel	289:294	arg1	China					364:368	PR China	361:368	PR China	361:368	A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium, designated strain CMS6Z-2T, was isolated from a surface-sterilized branch of Kandelia candel collected from the Maowei Sea, Guangxi Zhuang Autonomous Region, PR China.
33909548	5	17	theme	fatty	666:670	arg1	iso-C15 					683:690	iso-C15 	683:690	iso-C15 	683:690	The major fatty acids were iso-C15 : 0, C16 : 0 and C18 : 1 ω9c.
33909548	5	17	theme	fatty	666:670	arg1	acids					672:676	The major fatty acids	656:676	The major fatty acids	656:676	The major fatty acids were iso-C15 : 0, C16 : 0 and C18 : 1 ω9c.
33909548	7	18	theme	genomic	873:879	arg1	DNA					881:883	the genomic DNA	869:883	the genomic DNA	869:883	The G+C content of the genomic DNA was 74.1 mol%.
33909548	11	19	dep	Phycicoccus	1732:1742	arg1	flavus					1744:1749	flavus	1744:1749	flavus	1744:1749	On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain CMS6Z-2T represents a novel species of the genus Phycicoccus, for which the name Phycicoccus flavus sp.
33909548	3	20	dep	optimum	499:505	arg1	%					514:514	0-1.0 %	508:514	0-1.0 %	508:514	Strain CMS6Z-2T grew at 10-37 °C (optimum, 37 °C), pH 6.0-9.0 (optimum, pH 7.0-8.0) and in the presence of 0-10.0 % (w/v) NaCl (optimum, 0-1.0 %).
33909548	11	21	theme	Phycicoccus	1700:1710	arg1	species					1679:1685	a novel species	1671:1685	a novel species	1671:1685	On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain CMS6Z-2T represents a novel species of the genus Phycicoccus, for which the name Phycicoccus flavus sp.
33909548	8	22	theme	rRNA	928:931	arg1	genes					933:937	16S rRNA genes	924:937	16S rRNA genes	924:937	Comparative analysis of 16S rRNA genes showed that strain CMS6Z-2T should be assigned to the genus Phycicoccus and its closest relative was Phycicoccus endophyticus IP6SC6T with 98.3 % similarity.
33909548	7	23	theme	G+C	854:856	arg1	content					858:864	The G+C content	850:864	The G+C content of the genomic DNA	850:883	The G+C content of the genomic DNA was 74.1 mol%.
33909548	7	23	theme	G+C	854:856	arg1	%					897:897	74.1 mol%	889:897	74.1 mol%	889:897	The G+C content of the genomic DNA was 74.1 mol%.
33909548	9	24	theme	strain	1225:1230	arg1	CMS6Z-2T					1232:1239	strain CMS6Z-2T	1225:1239	strain CMS6Z-2T	1225:1239	Phylogenetic analyses based on 16S rRNA gene sequence and phylogenomic analysis based on core proteomes alignment revealed that strain CMS6Z-2T belonged to the genus Phycicoccus and formed a robust cluster with Phycicoccus endophyticus IP6SC6T within the genus Phycicoccus.
33909548	4	25	dep	peptidoglycan	596:608	arg1	the					592:594	the	592:594	the	592:594	Strain CMS6Z-2T possessed meso-diaminopimelic acid as the diamino acid of the peptidoglycan and MK-8 (H4) as the predominant menaquinone.
33909548	10	26	theme	nucleotide	1383:1392	arg1	identity					1394:1401	The average nucleotide identity	1371:1401	The average nucleotide identity value	1371:1407	The average nucleotide identity value and estimated digital DNA-DNA hybridization value between strain CMS6Z-2T and the type strain of Phycicoccus endophyticus were 81.5 and 23.9 %, respectively.
33909548	8	27	with	IP6SC6T	1065:1071	arg1	similarity					1085:1094	98.3 % similarity	1078:1094	98.3 % similarity	1078:1094	Comparative analysis of 16S rRNA genes showed that strain CMS6Z-2T should be assigned to the genus Phycicoccus and its closest relative was Phycicoccus endophyticus IP6SC6T with 98.3 % similarity.
33909548	0	28	theme	Phycicoccus	0:10	arg1	sp					19:20	Phycicoccus flavus sp	0:20	Phycicoccus flavus sp.	0:21	Phycicoccus flavus sp.
33909548	8	29	theme	strain	951:956	arg1	CMS6Z-2T					958:965	strain CMS6Z-2T	951:965	strain CMS6Z-2T	951:965	Comparative analysis of 16S rRNA genes showed that strain CMS6Z-2T should be assigned to the genus Phycicoccus and its closest relative was Phycicoccus endophyticus IP6SC6T with 98.3 % similarity.
33909548	11	30	theme	strain	1644:1649	arg1	CMS6Z-2T					1651:1658	strain CMS6Z-2T	1644:1658	strain CMS6Z-2T	1644:1658	On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain CMS6Z-2T represents a novel species of the genus Phycicoccus, for which the name Phycicoccus flavus sp.
33909548	10	31	theme	DNA-DNA	1431:1437	arg1	hybridization					1439:1451	digital DNA-DNA hybridization	1423:1451	estimated digital DNA-DNA hybridization value	1413:1457	The average nucleotide identity value and estimated digital DNA-DNA hybridization value between strain CMS6Z-2T and the type strain of Phycicoccus endophyticus were 81.5 and 23.9 %, respectively.
33909548	1	32	theme	candel	97:102	arg1	branch					78:83	branch	78:83	branch of Kandelia candel	78:102	nov., a novel endophytic actinobacterium isolated from branch of Kandelia candel.
33909548	10	33	theme	estimated	1413:1421	arg1	value					1453:1457	estimated digital DNA-DNA hybridization value	1413:1457	estimated digital DNA-DNA hybridization value	1413:1457	The average nucleotide identity value and estimated digital DNA-DNA hybridization value between strain CMS6Z-2T and the type strain of Phycicoccus endophyticus were 81.5 and 23.9 %, respectively.
33909548	2	34	theme	rod-shaped	175:184	arg1	actinobacterium					186:200	A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium	105:200	A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium	105:200	A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium, designated strain CMS6Z-2T, was isolated from a surface-sterilized branch of Kandelia candel collected from the Maowei Sea, Guangxi Zhuang Autonomous Region, PR China.
33909548	13	35	theme	type	1777:1780	arg1	CMS6Z-2T					1792:1799	CMS6Z-2T	1792:1799	CMS6Z-2T (=KCTC 49240T=CGMCC4.7549T)	1792:1827	The type strain is CMS6Z-2T (=KCTC 49240T=CGMCC4.7549T).
33909548	13	35	theme	type	1777:1780	arg1	strain					1782:1787	The type strain	1773:1787	The type strain	1773:1787	The type strain is CMS6Z-2T (=KCTC 49240T=CGMCC4.7549T).
33909548	2	36	theme	non-endospore-forming	149:169	arg1	actinobacterium					186:200	A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium	105:200	A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium	105:200	A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium, designated strain CMS6Z-2T, was isolated from a surface-sterilized branch of Kandelia candel collected from the Maowei Sea, Guangxi Zhuang Autonomous Region, PR China.
33909548	2	37	theme	strain	214:219	arg1	CMS6Z-2T					221:228	strain CMS6Z-2T	214:228	strain CMS6Z-2T	214:228	A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium, designated strain CMS6Z-2T, was isolated from a surface-sterilized branch of Kandelia candel collected from the Maowei Sea, Guangxi Zhuang Autonomous Region, PR China.
33909548	3	38	theme	pH	443:444	arg1	7.0-8.0					446:452	pH 7.0-8.0	443:452	pH 7.0-8.0	443:452	Strain CMS6Z-2T grew at 10-37 °C (optimum, 37 °C), pH 6.0-9.0 (optimum, pH 7.0-8.0) and in the presence of 0-10.0 % (w/v) NaCl (optimum, 0-1.0 %).
33909548	9	39	theme	robust	1288:1293	arg1	cluster					1295:1301	a robust cluster	1286:1301	a robust cluster	1286:1301	Phylogenetic analyses based on 16S rRNA gene sequence and phylogenomic analysis based on core proteomes alignment revealed that strain CMS6Z-2T belonged to the genus Phycicoccus and formed a robust cluster with Phycicoccus endophyticus IP6SC6T within the genus Phycicoccus.
33909548	10	40	theme	type	1491:1494	arg1	strain					1496:1501	the type strain	1487:1501	the type strain of Phycicoccus endophyticus	1487:1529	The average nucleotide identity value and estimated digital DNA-DNA hybridization value between strain CMS6Z-2T and the type strain of Phycicoccus endophyticus were 81.5 and 23.9 %, respectively.
33909548	1	41	theme	novel	31:35	arg1	actinobacterium					48:62	a novel endophytic actinobacterium	29:62	a novel endophytic actinobacterium isolated from branch of Kandelia candel	29:102	nov., a novel endophytic actinobacterium isolated from branch of Kandelia candel.
33909548	2	42	theme	Gram-stain-positive	107:125	arg1	actinobacterium					186:200	A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium	105:200	A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium	105:200	A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium, designated strain CMS6Z-2T, was isolated from a surface-sterilized branch of Kandelia candel collected from the Maowei Sea, Guangxi Zhuang Autonomous Region, PR China.
33909548	2	43	theme	PR	361:362	arg1	Region					353:358	Autonomous Region	342:358	Autonomous Region	342:358	A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium, designated strain CMS6Z-2T, was isolated from a surface-sterilized branch of Kandelia candel collected from the Maowei Sea, Guangxi Zhuang Autonomous Region, PR China.
33909548	2	43	theme	PR	361:362	arg1	China					364:368	PR China	361:368	PR China	361:368	A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium, designated strain CMS6Z-2T, was isolated from a surface-sterilized branch of Kandelia candel collected from the Maowei Sea, Guangxi Zhuang Autonomous Region, PR China.
33909548	10	44	theme	endophyticus	1518:1529	arg1	strain					1496:1501	the type strain	1487:1501	the type strain of Phycicoccus endophyticus	1487:1529	The average nucleotide identity value and estimated digital DNA-DNA hybridization value between strain CMS6Z-2T and the type strain of Phycicoccus endophyticus were 81.5 and 23.9 %, respectively.
33909548	10	44	theme	endophyticus	1518:1529	arg1	strain					1467:1472	strain CMS6Z-2T	1467:1481	strain CMS6Z-2T	1467:1481	The average nucleotide identity value and estimated digital DNA-DNA hybridization value between strain CMS6Z-2T and the type strain of Phycicoccus endophyticus were 81.5 and 23.9 %, respectively.
33909548	8	45	theme	Phycicoccus	1040:1050	arg1	IP6SC6T					1065:1071	Phycicoccus endophyticus IP6SC6T	1040:1071	Phycicoccus endophyticus IP6SC6T	1040:1071	Comparative analysis of 16S rRNA genes showed that strain CMS6Z-2T should be assigned to the genus Phycicoccus and its closest relative was Phycicoccus endophyticus IP6SC6T with 98.3 % similarity.
33909548	2	46	theme	non-motile	137:146	arg1	actinobacterium					186:200	A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium	105:200	A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium	105:200	A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium, designated strain CMS6Z-2T, was isolated from a surface-sterilized branch of Kandelia candel collected from the Maowei Sea, Guangxi Zhuang Autonomous Region, PR China.
33909548	2	47	theme	candel	289:294	arg1	branch					270:275	a surface-sterilized branch	249:275	a surface-sterilized branch of Kandelia candel collected from the Maowei Sea, Guangxi Zhuang Autonomous Region, PR China	249:368	A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium, designated strain CMS6Z-2T, was isolated from a surface-sterilized branch of Kandelia candel collected from the Maowei Sea, Guangxi Zhuang Autonomous Region, PR China.
33909548	9	48	theme	genus	1352:1356	arg1	Phycicoccus					1358:1368	the genus Phycicoccus	1348:1368	the genus Phycicoccus	1348:1368	Phylogenetic analyses based on 16S rRNA gene sequence and phylogenomic analysis based on core proteomes alignment revealed that strain CMS6Z-2T belonged to the genus Phycicoccus and formed a robust cluster with Phycicoccus endophyticus IP6SC6T within the genus Phycicoccus.
33909548	4	49	theme	diamino	576:582	arg1	acid					584:587	the diamino acid	572:587	the diamino acid of the peptidoglycan and MK-8 (H4) as the predominant menaquinone	572:653	Strain CMS6Z-2T possessed meso-diaminopimelic acid as the diamino acid of the peptidoglycan and MK-8 (H4) as the predominant menaquinone.
33909548	4	49	theme	diamino	576:582	arg1	acid					564:567	meso-diaminopimelic acid	544:567	meso-diaminopimelic acid	544:567	Strain CMS6Z-2T possessed meso-diaminopimelic acid as the diamino acid of the peptidoglycan and MK-8 (H4) as the predominant menaquinone.
33909548	2	50	theme	aerobic	128:134	arg1	actinobacterium					186:200	A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium	105:200	A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium	105:200	A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium, designated strain CMS6Z-2T, was isolated from a surface-sterilized branch of Kandelia candel collected from the Maowei Sea, Guangxi Zhuang Autonomous Region, PR China.
33909548	8	51	theme	98.3 	1078:1082	arg1	%					1083:1083	%	1083:1083	%	1083:1083	Comparative analysis of 16S rRNA genes showed that strain CMS6Z-2T should be assigned to the genus Phycicoccus and its closest relative was Phycicoccus endophyticus IP6SC6T with 98.3 % similarity.
33909548	2	52	theme	Maowei	315:320	arg1	Sea					322:324	the Maowei Sea	311:324	the Maowei Sea	311:324	A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium, designated strain CMS6Z-2T, was isolated from a surface-sterilized branch of Kandelia candel collected from the Maowei Sea, Guangxi Zhuang Autonomous Region, PR China.
33909548	4	53	theme	meso-diaminopimelic	544:562	arg1	acid					584:587	the diamino acid	572:587	the diamino acid of the peptidoglycan and MK-8 (H4) as the predominant menaquinone	572:653	Strain CMS6Z-2T possessed meso-diaminopimelic acid as the diamino acid of the peptidoglycan and MK-8 (H4) as the predominant menaquinone.
33909548	4	53	theme	meso-diaminopimelic	544:562	arg1	acid					564:567	meso-diaminopimelic acid	544:567	meso-diaminopimelic acid	544:567	Strain CMS6Z-2T possessed meso-diaminopimelic acid as the diamino acid of the peptidoglycan and MK-8 (H4) as the predominant menaquinone.
33909548	1	54	attach	isolated	64:71	arg1	branch					78:83	branch	78:83	branch of Kandelia candel	78:102	nov., a novel endophytic actinobacterium isolated from branch of Kandelia candel.
33909548	1	54	attach	isolated	64:71	arg2	actinobacterium					48:62	a novel endophytic actinobacterium	29:62	a novel endophytic actinobacterium isolated from branch of Kandelia candel	29:102	nov., a novel endophytic actinobacterium isolated from branch of Kandelia candel.
33909548	9	55	theme	rRNA	1132:1135	arg1	sequence					1142:1149	16S rRNA gene sequence	1128:1149	16S rRNA gene sequence	1128:1149	Phylogenetic analyses based on 16S rRNA gene sequence and phylogenomic analysis based on core proteomes alignment revealed that strain CMS6Z-2T belonged to the genus Phycicoccus and formed a robust cluster with Phycicoccus endophyticus IP6SC6T within the genus Phycicoccus.
33909548	5	56	theme	 1	713:714	arg1	ω9c					716:718	 1 ω9c	713:718	 1 ω9c	713:718	The major fatty acids were iso-C15 : 0, C16 : 0 and C18 : 1 ω9c.
33909548	9	57	theme	sequence	1142:1149	arg1	alignment					1201:1209	16S rRNA gene sequence and phylogenomic analysis based on core proteomes alignment	1128:1209	16S rRNA gene sequence and phylogenomic analysis based on core proteomes alignment	1128:1209	Phylogenetic analyses based on 16S rRNA gene sequence and phylogenomic analysis based on core proteomes alignment revealed that strain CMS6Z-2T belonged to the genus Phycicoccus and formed a robust cluster with Phycicoccus endophyticus IP6SC6T within the genus Phycicoccus.
33909548	11	58	theme	novel	1673:1677	arg1	species					1679:1685	a novel species	1671:1685	a novel species	1671:1685	On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain CMS6Z-2T represents a novel species of the genus Phycicoccus, for which the name Phycicoccus flavus sp.
33909548	9	59	theme	phylogenomic	1155:1166	arg1	analysis					1168:1175	phylogenomic analysis	1155:1175	phylogenomic analysis based on core proteomes	1155:1199	Phylogenetic analyses based on 16S rRNA gene sequence and phylogenomic analysis based on core proteomes alignment revealed that strain CMS6Z-2T belonged to the genus Phycicoccus and formed a robust cluster with Phycicoccus endophyticus IP6SC6T within the genus Phycicoccus.
33909548	1	60	dep	actinobacterium	48:62	arg1	nov.					23:26	nov.	23:26	nov.	23:26	nov., a novel endophytic actinobacterium isolated from branch of Kandelia candel.
33909548	11	61	theme	genus	1694:1698	arg1	Phycicoccus					1700:1710	the genus Phycicoccus	1690:1710	the genus Phycicoccus	1690:1710	On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain CMS6Z-2T represents a novel species of the genus Phycicoccus, for which the name Phycicoccus flavus sp.
33909548	5	62	theme	major	660:664	arg1	iso-C15 					683:690	iso-C15 	683:690	iso-C15 	683:690	The major fatty acids were iso-C15 : 0, C16 : 0 and C18 : 1 ω9c.
33909548	5	62	theme	major	660:664	arg1	acids					672:676	The major fatty acids	656:676	The major fatty acids	656:676	The major fatty acids were iso-C15 : 0, C16 : 0 and C18 : 1 ω9c.
33909548	2	63	theme	surface-sterilized	251:268	arg1	branch					270:275	a surface-sterilized branch	249:275	a surface-sterilized branch of Kandelia candel collected from the Maowei Sea, Guangxi Zhuang Autonomous Region, PR China	249:368	A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium, designated strain CMS6Z-2T, was isolated from a surface-sterilized branch of Kandelia candel collected from the Maowei Sea, Guangxi Zhuang Autonomous Region, PR China.
33909548	7	64	theme	74.1 mol	889:896	arg1	content					858:864	The G+C content	850:864	The G+C content of the genomic DNA	850:883	The G+C content of the genomic DNA was 74.1 mol%.
33909548	7	64	theme	74.1 mol	889:896	arg1	%					897:897	74.1 mol%	889:897	74.1 mol%	889:897	The G+C content of the genomic DNA was 74.1 mol%.
33909548	4	65	theme	peptidoglycan	596:608	arg1	acid					584:587	the diamino acid	572:587	the diamino acid of the peptidoglycan and MK-8 (H4) as the predominant menaquinone	572:653	Strain CMS6Z-2T possessed meso-diaminopimelic acid as the diamino acid of the peptidoglycan and MK-8 (H4) as the predominant menaquinone.
33909548	4	65	theme	peptidoglycan	596:608	arg1	acid					564:567	meso-diaminopimelic acid	544:567	meso-diaminopimelic acid	544:567	Strain CMS6Z-2T possessed meso-diaminopimelic acid as the diamino acid of the peptidoglycan and MK-8 (H4) as the predominant menaquinone.
33909548	13	66	theme	=KCTC	1802:1806	arg1	CMS6Z-2T					1792:1799	CMS6Z-2T	1792:1799	CMS6Z-2T (=KCTC 49240T=CGMCC4.7549T)	1792:1827	The type strain is CMS6Z-2T (=KCTC 49240T=CGMCC4.7549T).
33909548	13	66	theme	=KCTC	1802:1806	arg1	49240T=CGMCC4.7549T					1808:1826	=KCTC 49240T=CGMCC4.7549T	1802:1826	=KCTC 49240T=CGMCC4.7549T	1802:1826	The type strain is CMS6Z-2T (=KCTC 49240T=CGMCC4.7549T).
33909548	9	67	theme	core	1186:1189	arg1	proteomes					1191:1199	core proteomes	1186:1199	core proteomes	1186:1199	Phylogenetic analyses based on 16S rRNA gene sequence and phylogenomic analysis based on core proteomes alignment revealed that strain CMS6Z-2T belonged to the genus Phycicoccus and formed a robust cluster with Phycicoccus endophyticus IP6SC6T within the genus Phycicoccus.
33909548	8	68	theme	16S	924:926	arg1	genes					933:937	16S rRNA genes	924:937	16S rRNA genes	924:937	Comparative analysis of 16S rRNA genes showed that strain CMS6Z-2T should be assigned to the genus Phycicoccus and its closest relative was Phycicoccus endophyticus IP6SC6T with 98.3 % similarity.
33909548	7	69	theme	DNA	881:883	arg1	content					858:864	The G+C content	850:864	The G+C content of the genomic DNA	850:883	The G+C content of the genomic DNA was 74.1 mol%.
33909548	7	69	theme	DNA	881:883	arg1	%					897:897	74.1 mol%	889:897	74.1 mol%	889:897	The G+C content of the genomic DNA was 74.1 mol%.
33909548	2	70	attach	isolated	235:242	arg1	branch					270:275	a surface-sterilized branch	249:275	a surface-sterilized branch of Kandelia candel collected from the Maowei Sea, Guangxi Zhuang Autonomous Region, PR China	249:368	A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium, designated strain CMS6Z-2T, was isolated from a surface-sterilized branch of Kandelia candel collected from the Maowei Sea, Guangxi Zhuang Autonomous Region, PR China.
33909548	2	70	attach	isolated	235:242	arg2	actinobacterium					186:200	A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium	105:200	A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium	105:200	A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium, designated strain CMS6Z-2T, was isolated from a surface-sterilized branch of Kandelia candel collected from the Maowei Sea, Guangxi Zhuang Autonomous Region, PR China.
33909548	4	71	theme	MK-8	614:617	arg1	acid					584:587	the diamino acid	572:587	the diamino acid of the peptidoglycan and MK-8 (H4) as the predominant menaquinone	572:653	Strain CMS6Z-2T possessed meso-diaminopimelic acid as the diamino acid of the peptidoglycan and MK-8 (H4) as the predominant menaquinone.
33909548	4	71	theme	MK-8	614:617	arg1	acid					564:567	meso-diaminopimelic acid	544:567	meso-diaminopimelic acid	544:567	Strain CMS6Z-2T possessed meso-diaminopimelic acid as the diamino acid of the peptidoglycan and MK-8 (H4) as the predominant menaquinone.
33909548	8	72	theme	genes	933:937	arg1	analysis					912:919	Comparative analysis	900:919	Comparative analysis of 16S rRNA genes	900:937	Comparative analysis of 16S rRNA genes showed that strain CMS6Z-2T should be assigned to the genus Phycicoccus and its closest relative was Phycicoccus endophyticus IP6SC6T with 98.3 % similarity.
33909548	1	73	theme	endophytic	37:46	arg1	actinobacterium					48:62	a novel endophytic actinobacterium	29:62	a novel endophytic actinobacterium isolated from branch of Kandelia candel	29:102	nov., a novel endophytic actinobacterium isolated from branch of Kandelia candel.
33909548	8	74	dep	Phycicoccus	1040:1050	arg1	endophyticus					1052:1063	endophyticus	1052:1063	endophyticus	1052:1063	Comparative analysis of 16S rRNA genes showed that strain CMS6Z-2T should be assigned to the genus Phycicoccus and its closest relative was Phycicoccus endophyticus IP6SC6T with 98.3 % similarity.
33909548	10	75	theme	identity	1394:1401	arg1	value					1403:1407	The average nucleotide identity value	1371:1407	The average nucleotide identity value	1371:1407	The average nucleotide identity value and estimated digital DNA-DNA hybridization value between strain CMS6Z-2T and the type strain of Phycicoccus endophyticus were 81.5 and 23.9 %, respectively.
33909548	0	76	theme	flavus	12:17	arg1	sp					19:20	Phycicoccus flavus sp	0:20	Phycicoccus flavus sp.	0:21	Phycicoccus flavus sp.
33909548	11	77	theme	name	1727:1730	arg1	sp					1751:1752	the name Phycicoccus flavus sp	1723:1752	the name Phycicoccus flavus sp	1723:1752	On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain CMS6Z-2T represents a novel species of the genus Phycicoccus, for which the name Phycicoccus flavus sp.
33909548	10	78	theme	average	1375:1381	arg1	identity					1394:1401	The average nucleotide identity	1371:1401	The average nucleotide identity value	1371:1407	The average nucleotide identity value and estimated digital DNA-DNA hybridization value between strain CMS6Z-2T and the type strain of Phycicoccus endophyticus were 81.5 and 23.9 %, respectively.
33909548	4	79	theme	predominant	631:641	arg1	menaquinone					643:653	the predominant menaquinone	627:653	the predominant menaquinone	627:653	Strain CMS6Z-2T possessed meso-diaminopimelic acid as the diamino acid of the peptidoglycan and MK-8 (H4) as the predominant menaquinone.
33909548	1	80	theme	Kandelia	88:95	arg1	candel					97:102	Kandelia candel	88:102	Kandelia candel	88:102	nov., a novel endophytic actinobacterium isolated from branch of Kandelia candel.
33909548	10	81	theme	digital	1423:1429	arg1	hybridization					1439:1451	digital DNA-DNA hybridization	1423:1451	estimated digital DNA-DNA hybridization value	1413:1457	The average nucleotide identity value and estimated digital DNA-DNA hybridization value between strain CMS6Z-2T and the type strain of Phycicoccus endophyticus were 81.5 and 23.9 %, respectively.
33909548	9	82	theme	genus	1257:1261	arg1	Phycicoccus					1263:1273	the genus Phycicoccus	1253:1273	the genus Phycicoccus	1253:1273	Phylogenetic analyses based on 16S rRNA gene sequence and phylogenomic analysis based on core proteomes alignment revealed that strain CMS6Z-2T belonged to the genus Phycicoccus and formed a robust cluster with Phycicoccus endophyticus IP6SC6T within the genus Phycicoccus.
33909548	6	83	theme	polar	725:729	arg1	lipids					731:736	The polar lipids	721:736	The polar lipids	721:736	The polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and two unknown phospholipids.
33909548	11	84	theme	chemotaxonomic	1612:1625	arg1	characteristics					1627:1641	phylogenetic, phenotypic and chemotaxonomic characteristics	1583:1641	phylogenetic, phenotypic and chemotaxonomic characteristics	1583:1641	On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain CMS6Z-2T represents a novel species of the genus Phycicoccus, for which the name Phycicoccus flavus sp.
33909548	8	85	theme	genus	993:997	arg1	Phycicoccus					999:1009	the genus Phycicoccus	989:1009	the genus Phycicoccus	989:1009	Comparative analysis of 16S rRNA genes showed that strain CMS6Z-2T should be assigned to the genus Phycicoccus and its closest relative was Phycicoccus endophyticus IP6SC6T with 98.3 % similarity.
33909548	2	86	theme	Autonomous	342:351	arg1	Region					353:358	Autonomous Region	342:358	Autonomous Region	342:358	A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium, designated strain CMS6Z-2T, was isolated from a surface-sterilized branch of Kandelia candel collected from the Maowei Sea, Guangxi Zhuang Autonomous Region, PR China.
33909548	2	86	theme	Autonomous	342:351	arg1	China					364:368	PR China	361:368	PR China	361:368	A Gram-stain-positive, aerobic, non-motile, non-endospore-forming and rod-shaped actinobacterium, designated strain CMS6Z-2T, was isolated from a surface-sterilized branch of Kandelia candel collected from the Maowei Sea, Guangxi Zhuang Autonomous Region, PR China.
33909548	4	87	theme	Strain	518:523	arg1	CMS6Z-2T					525:532	Strain CMS6Z-2T	518:532	Strain CMS6Z-2T	518:532	Strain CMS6Z-2T possessed meso-diaminopimelic acid as the diamino acid of the peptidoglycan and MK-8 (H4) as the predominant menaquinone.
33909548	10	88	theme	hybridization	1439:1451	arg1	value					1453:1457	estimated digital DNA-DNA hybridization value	1413:1457	estimated digital DNA-DNA hybridization value	1413:1457	The average nucleotide identity value and estimated digital DNA-DNA hybridization value between strain CMS6Z-2T and the type strain of Phycicoccus endophyticus were 81.5 and 23.9 %, respectively.
33909548	5	89	dep	ω9c	716:718	arg1	C18 					708:711	C18 	708:711	C18 	708:711	The major fatty acids were iso-C15 : 0, C16 : 0 and C18 : 1 ω9c.
33909548	5	89	dep	ω9c	716:718	arg1	 0					701:702	 0	701:702	 0	701:702	The major fatty acids were iso-C15 : 0, C16 : 0 and C18 : 1 ω9c.
33909548	5	89	dep	ω9c	716:718	arg1	C16 					696:699	C16 	696:699	C16 	696:699	The major fatty acids were iso-C15 : 0, C16 : 0 and C18 : 1 ω9c.
33909548	11	90	dep	characteristics	1627:1641	arg1	basis					1574:1578	basis	1574:1578	basis	1574:1578	On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain CMS6Z-2T represents a novel species of the genus Phycicoccus, for which the name Phycicoccus flavus sp.
33909548	11	90	dep	characteristics	1627:1641	arg1	the					1570:1572	the	1570:1572	the	1570:1572	On the basis of phylogenetic, phenotypic and chemotaxonomic characteristics, strain CMS6Z-2T represents a novel species of the genus Phycicoccus, for which the name Phycicoccus flavus sp.
33909548	8	91	theme	closest	1019:1025	arg1	relative					1027:1034	its closest relative	1015:1034	its closest relative	1015:1034	Comparative analysis of 16S rRNA genes showed that strain CMS6Z-2T should be assigned to the genus Phycicoccus and its closest relative was Phycicoccus endophyticus IP6SC6T with 98.3 % similarity.
33909548	3	92	dep	optimum	434:440	arg1	7.0-8.0					446:452	pH 7.0-8.0	443:452	pH 7.0-8.0	443:452	Strain CMS6Z-2T grew at 10-37 °C (optimum, 37 °C), pH 6.0-9.0 (optimum, pH 7.0-8.0) and in the presence of 0-10.0 % (w/v) NaCl (optimum, 0-1.0 %).
33909548	9	93	theme	Phycicoccus	1308:1318	arg1	IP6SC6T					1333:1339	Phycicoccus endophyticus IP6SC6T	1308:1339	Phycicoccus endophyticus IP6SC6T within the genus Phycicoccus	1308:1368	Phylogenetic analyses based on 16S rRNA gene sequence and phylogenomic analysis based on core proteomes alignment revealed that strain CMS6Z-2T belonged to the genus Phycicoccus and formed a robust cluster with Phycicoccus endophyticus IP6SC6T within the genus Phycicoccus.
33105388	7	0	theme	Ex	1274:1275	arg1	mice					1277:1280	the 5011 Ex mice	1265:1280	the 5011 Ex mice	1265:1280	Both HFD Ex and 5011 Ex mice had reduced endogenous glucose during the glucose tolerance test, whereas only the 5011 Sed and the 5011 Ex mice had improved glucose disposal after the 4-wk experimental period when compared with HFD Sed and HFD Ex mice.
33105388	4	1	theme	isotope	681:687	arg1	test					707:710	A stable isotope glucose tolerance test	672:710	A stable isotope glucose tolerance test	672:710	A stable isotope glucose tolerance test was performed before and after the 4-wk wheel running period to determine changes in endogenous glucose production and glucose disposal.
33105388	8	2	theme	Ex	1396:1397	arg1	mice					1399:1402	5011 Ex mice	1391:1402	5011 Ex mice	1391:1402	5011 Ex mice had increased Pgc1-α and Tfam expression in skeletal muscle when compared with HFD Ex mice, whereas Pdk4 expression was reduced in the liver of HFD Ex and 5011 Ex mice.
33105388	1	3	from	glucose	276:282	arg1	mice					330:333	diet-induced obese male mice	306:333	diet-induced obese male mice	306:333	PURPOSE The purpose of this study was to determine the effect of an ethanolic extract of Artemisia dracunculus L. (5011) combined with exercise on in vivo glucose and fat metabolism in diet-induced obese male mice.
33105388	2	4	dep	HFD	455:457	arg1	Ex					459:460	HFD Ex	455:460	HFD Ex	455:460	METHODS After 8 wk of high-fat diet (HFD) feeding, 52 mice were randomly allocated to a voluntary wheel running group (HFD Ex), a 5011 + HFD sedentary group (5011 Sed), a 5011 + HFD Ex (5011 Ex), or an HFD sedentary group (HFD Sed) for 4 wk.
33105388	2	5	theme	sedentary	477:485	arg1	group					487:491	a 5011 + HFD sedentary group	464:491	group	487:491	METHODS After 8 wk of high-fat diet (HFD) feeding, 52 mice were randomly allocated to a voluntary wheel running group (HFD Ex), a 5011 + HFD sedentary group (5011 Sed), a 5011 + HFD Ex (5011 Ex), or an HFD sedentary group (HFD Sed) for 4 wk.
33105388	9	6	theme	ethanolic	1622:1630	arg1	extract					1632:1638	an ethanolic extract	1619:1638	an ethanolic extract of A. dracunculus L., with a history of medicinal use	1619:1692	CONCLUSIONS Our study demonstrates that 5011, an ethanolic extract of A. dracunculus L., with a history of medicinal use, enhances the metabolic benefits of exercise to improve in vivo fat and glucose metabolism.
33105388	9	6	theme	ethanolic	1622:1630	arg1	5011					1613:1616	5011	1613:1616	5011	1613:1616	CONCLUSIONS Our study demonstrates that 5011, an ethanolic extract of A. dracunculus L., with a history of medicinal use, enhances the metabolic benefits of exercise to improve in vivo fat and glucose metabolism.
33105388	6	7	theme	fat	1070:1072	arg1	oxidation					1074:1082	increased fat oxidation	1060:1082	increased fat oxidation	1060:1082	RESULTS When compared with HFD Ex mice, 5011 Ex mice had increased fat oxidation during speed- and distance-matched wheel running bouts.
33105388	7	8	theme	tolerance	1219:1227	arg1	test					1229:1232	the glucose tolerance test	1207:1232	the glucose tolerance test	1207:1232	Both HFD Ex and 5011 Ex mice had reduced endogenous glucose during the glucose tolerance test, whereas only the 5011 Sed and the 5011 Ex mice had improved glucose disposal after the 4-wk experimental period when compared with HFD Sed and HFD Ex mice.
33105388	7	9	contain	had	1169:1171	arg2	glucose					1192:1198	reduced endogenous glucose	1173:1198	reduced endogenous glucose	1173:1198	Both HFD Ex and 5011 Ex mice had reduced endogenous glucose during the glucose tolerance test, whereas only the 5011 Sed and the 5011 Ex mice had improved glucose disposal after the 4-wk experimental period when compared with HFD Sed and HFD Ex mice.
33105388	7	9	contain	had	1169:1171	arg1	mice					1164:1167	Both HFD Ex and 5011 Ex mice	1140:1167	Both HFD Ex and 5011 Ex mice	1140:1167	Both HFD Ex and 5011 Ex mice had reduced endogenous glucose during the glucose tolerance test, whereas only the 5011 Sed and the 5011 Ex mice had improved glucose disposal after the 4-wk experimental period when compared with HFD Sed and HFD Ex mice.
33105388	8	10	theme	HFD	1483:1485	arg1	mice					1490:1493	HFD Ex mice	1483:1493	HFD Ex mice	1483:1493	5011 Ex mice had increased Pgc1-α and Tfam expression in skeletal muscle when compared with HFD Ex mice, whereas Pdk4 expression was reduced in the liver of HFD Ex and 5011 Ex mice.
33105388	7	11	dep	had	1169:1171	arg1	whereas					1235:1241	whereas	1235:1241	whereas	1235:1241	Both HFD Ex and 5011 Ex mice had reduced endogenous glucose during the glucose tolerance test, whereas only the 5011 Sed and the 5011 Ex mice had improved glucose disposal after the 4-wk experimental period when compared with HFD Sed and HFD Ex mice.
33105388	2	12	theme	HFD	559:561	arg1	group					552:556	an HFD sedentary group	535:556	an HFD sedentary group (HFD Sed)	535:566	METHODS After 8 wk of high-fat diet (HFD) feeding, 52 mice were randomly allocated to a voluntary wheel running group (HFD Ex), a 5011 + HFD sedentary group (5011 Sed), a 5011 + HFD Ex (5011 Ex), or an HFD sedentary group (HFD Sed) for 4 wk.
33105388	2	12	theme	HFD	559:561	arg1	Sed					563:565	HFD Sed	559:565	HFD Sed	559:565	METHODS After 8 wk of high-fat diet (HFD) feeding, 52 mice were randomly allocated to a voluntary wheel running group (HFD Ex), a 5011 + HFD sedentary group (5011 Sed), a 5011 + HFD Ex (5011 Ex), or an HFD sedentary group (HFD Sed) for 4 wk.
33105388	6	13	theme	Ex	1048:1049	arg1	mice					1051:1054	5011 Ex mice	1043:1054	5011 Ex mice	1043:1054	RESULTS When compared with HFD Ex mice, 5011 Ex mice had increased fat oxidation during speed- and distance-matched wheel running bouts.
33105388	9	14	theme	metabolic	1708:1716	arg1	benefits					1718:1725	the metabolic benefits	1704:1725	the metabolic benefits of exercise	1704:1737	CONCLUSIONS Our study demonstrates that 5011, an ethanolic extract of A. dracunculus L., with a history of medicinal use, enhances the metabolic benefits of exercise to improve in vivo fat and glucose metabolism.
33105388	5	15	theme	genes	882:886	arg1	analysis					870:877	an analysis	867:877	an analysis of genes and proteins associated with the early response to exercise and exercise adaptations in skeletal muscle and liver	867:1000	We also performed an analysis of genes and proteins associated with the early response to exercise and exercise adaptations in skeletal muscle and liver.
33105388	4	16	theme	4-wk	747:750	arg1	running					758:764	the 4-wk wheel running	743:764	the 4-wk wheel running period	743:771	A stable isotope glucose tolerance test was performed before and after the 4-wk wheel running period to determine changes in endogenous glucose production and glucose disposal.
33105388	0	17	from	Benefits	72:79	arg1	Mice					115:118	Diet-induced Obese Mice	96:118	Diet-induced Obese Mice	96:118	An Ethanolic Extract of Artemisia dracunculus L. Enhances the Metabolic Benefits of Exercise in Diet-induced Obese Mice.
33105388	7	18	theme	reduced	1173:1179	arg1	glucose					1192:1198	reduced endogenous glucose	1173:1198	reduced endogenous glucose	1173:1198	Both HFD Ex and 5011 Ex mice had reduced endogenous glucose during the glucose tolerance test, whereas only the 5011 Sed and the 5011 Ex mice had improved glucose disposal after the 4-wk experimental period when compared with HFD Sed and HFD Ex mice.
33105388	6	19	dep	RESULTS	1003:1009	arg1	had					1056:1058	had	1056:1058	had increased fat oxidation during speed- and distance-matched wheel running bouts	1056:1137	RESULTS When compared with HFD Ex mice, 5011 Ex mice had increased fat oxidation during speed- and distance-matched wheel running bouts.
33105388	5	20	theme	proteins	892:899	arg1	analysis					870:877	an analysis	867:877	an analysis of genes and proteins associated with the early response to exercise and exercise adaptations in skeletal muscle and liver	867:1000	We also performed an analysis of genes and proteins associated with the early response to exercise and exercise adaptations in skeletal muscle and liver.
33105388	9	21	dep	A.	1643:1644	arg1	L.					1658:1659	A. dracunculus L.	1643:1659	A. dracunculus L.	1643:1659	CONCLUSIONS Our study demonstrates that 5011, an ethanolic extract of A. dracunculus L., with a history of medicinal use, enhances the metabolic benefits of exercise to improve in vivo fat and glucose metabolism.
33105388	1	22	theme	ethanolic	189:197	arg1	extract					199:205	an ethanolic extract	186:205	an ethanolic extract of Artemisia dracunculus L	186:232	PURPOSE The purpose of this study was to determine the effect of an ethanolic extract of Artemisia dracunculus L. (5011) combined with exercise on in vivo glucose and fat metabolism in diet-induced obese male mice.
33105388	6	23	theme	wheel	1119:1123	arg1	running					1125:1131	wheel running	1119:1131	speed- and distance-matched wheel running bouts	1091:1137	RESULTS When compared with HFD Ex mice, 5011 Ex mice had increased fat oxidation during speed- and distance-matched wheel running bouts.
33105388	4	24	theme	tolerance	697:705	arg1	test					707:710	A stable isotope glucose tolerance test	672:710	A stable isotope glucose tolerance test	672:710	A stable isotope glucose tolerance test was performed before and after the 4-wk wheel running period to determine changes in endogenous glucose production and glucose disposal.
33105388	7	25	theme	Ex	1382:1383	arg1	mice					1385:1388	HFD Sed and HFD Ex mice	1366:1388	mice	1385:1388	Both HFD Ex and 5011 Ex mice had reduced endogenous glucose during the glucose tolerance test, whereas only the 5011 Sed and the 5011 Ex mice had improved glucose disposal after the 4-wk experimental period when compared with HFD Sed and HFD Ex mice.
33105388	6	26	theme	HFD	1030:1032	arg1	mice					1037:1040	HFD Ex mice	1030:1040	HFD Ex mice	1030:1040	RESULTS When compared with HFD Ex mice, 5011 Ex mice had increased fat oxidation during speed- and distance-matched wheel running bouts.
33105388	9	27	theme	use	1690:1692	arg1	history					1669:1675	a history	1667:1675	a history of medicinal use	1667:1692	CONCLUSIONS Our study demonstrates that 5011, an ethanolic extract of A. dracunculus L., with a history of medicinal use, enhances the metabolic benefits of exercise to improve in vivo fat and glucose metabolism.
33105388	3	28	theme	indirect	650:657	arg1	calorimetry					659:669	indirect calorimetry	650:669	indirect calorimetry	650:669	Real-time energy expenditure and substrate utilization were measured by indirect calorimetry.
33105388	9	29	dep	in	1750:1751	arg1	vivo					1753:1756	vivo	1753:1756	vivo	1753:1756	CONCLUSIONS Our study demonstrates that 5011, an ethanolic extract of A. dracunculus L., with a history of medicinal use, enhances the metabolic benefits of exercise to improve in vivo fat and glucose metabolism.
33105388	7	30	theme	Ex	1149:1150	arg1	mice					1164:1167	Both HFD Ex and 5011 Ex mice	1140:1167	Both HFD Ex and 5011 Ex mice	1140:1167	Both HFD Ex and 5011 Ex mice had reduced endogenous glucose during the glucose tolerance test, whereas only the 5011 Sed and the 5011 Ex mice had improved glucose disposal after the 4-wk experimental period when compared with HFD Sed and HFD Ex mice.
33105388	3	31	theme	energy	588:593	arg1	expenditure					595:605	Real-time energy expenditure	578:605	Real-time energy expenditure	578:605	Real-time energy expenditure and substrate utilization were measured by indirect calorimetry.
33105388	8	32	dep	had	1404:1406	arg1	whereas					1496:1502	whereas	1496:1502	whereas	1496:1502	5011 Ex mice had increased Pgc1-α and Tfam expression in skeletal muscle when compared with HFD Ex mice, whereas Pdk4 expression was reduced in the liver of HFD Ex and 5011 Ex mice.
33105388	7	33	theme	HFD	1366:1368	arg1	mice					1385:1388	HFD Sed and HFD Ex mice	1366:1388	mice	1385:1388	Both HFD Ex and 5011 Ex mice had reduced endogenous glucose during the glucose tolerance test, whereas only the 5011 Sed and the 5011 Ex mice had improved glucose disposal after the 4-wk experimental period when compared with HFD Sed and HFD Ex mice.
33105388	4	34	theme	endogenous	797:806	arg1	production					816:825	endogenous glucose production	797:825	endogenous glucose production	797:825	A stable isotope glucose tolerance test was performed before and after the 4-wk wheel running period to determine changes in endogenous glucose production and glucose disposal.
33105388	2	35	theme	HFD	538:540	arg1	group					552:556	an HFD sedentary group	535:556	an HFD sedentary group (HFD Sed)	535:566	METHODS After 8 wk of high-fat diet (HFD) feeding, 52 mice were randomly allocated to a voluntary wheel running group (HFD Ex), a 5011 + HFD sedentary group (5011 Sed), a 5011 + HFD Ex (5011 Ex), or an HFD sedentary group (HFD Sed) for 4 wk.
33105388	2	35	theme	HFD	538:540	arg1	Sed					563:565	HFD Sed	559:565	HFD Sed	559:565	METHODS After 8 wk of high-fat diet (HFD) feeding, 52 mice were randomly allocated to a voluntary wheel running group (HFD Ex), a 5011 + HFD sedentary group (5011 Sed), a 5011 + HFD Ex (5011 Ex), or an HFD sedentary group (HFD Sed) for 4 wk.
33105388	8	36	theme	Ex	1552:1553	arg1	mice					1567:1570	HFD Ex and 5011 Ex mice	1548:1570	HFD Ex and 5011 Ex mice	1548:1570	5011 Ex mice had increased Pgc1-α and Tfam expression in skeletal muscle when compared with HFD Ex mice, whereas Pdk4 expression was reduced in the liver of HFD Ex and 5011 Ex mice.
33105388	1	37	theme	study	149:153	arg1	purpose					133:139	The purpose	129:139	The purpose of this study	129:153	PURPOSE The purpose of this study was to determine the effect of an ethanolic extract of Artemisia dracunculus L. (5011) combined with exercise on in vivo glucose and fat metabolism in diet-induced obese male mice.
33105388	4	38	theme	running	758:764	arg1	period					766:771	the 4-wk wheel running period	743:771	the 4-wk wheel running period	743:771	A stable isotope glucose tolerance test was performed before and after the 4-wk wheel running period to determine changes in endogenous glucose production and glucose disposal.
33105388	8	39	contain	had	1404:1406	arg1	mice					1399:1402	5011 Ex mice	1391:1402	5011 Ex mice	1391:1402	5011 Ex mice had increased Pgc1-α and Tfam expression in skeletal muscle when compared with HFD Ex mice, whereas Pdk4 expression was reduced in the liver of HFD Ex and 5011 Ex mice.
33105388	8	39	contain	had	1404:1406	arg2	expression					1434:1443	increased Pgc1-α and Tfam expression	1408:1443	increased Pgc1-α and Tfam expression in skeletal muscle	1408:1462	5011 Ex mice had increased Pgc1-α and Tfam expression in skeletal muscle when compared with HFD Ex mice, whereas Pdk4 expression was reduced in the liver of HFD Ex and 5011 Ex mice.
33105388	1	40	dep	PURPOSE	121:127	arg1	was					155:157	was	155:157	was to determine the effect of an ethanolic extract of Artemisia dracunculus L	155:232	PURPOSE The purpose of this study was to determine the effect of an ethanolic extract of Artemisia dracunculus L. (5011) combined with exercise on in vivo glucose and fat metabolism in diet-induced obese male mice.
33105388	0	41	theme	Exercise	84:91	arg1	Benefits					72:79	the Metabolic Benefits	58:79	the Metabolic Benefits of Exercise in Diet-induced Obese Mice	58:118	An Ethanolic Extract of Artemisia dracunculus L. Enhances the Metabolic Benefits of Exercise in Diet-induced Obese Mice.
33105388	1	42	theme	diet-induced	306:317	arg1	mice					330:333	diet-induced obese male mice	306:333	diet-induced obese male mice	306:333	PURPOSE The purpose of this study was to determine the effect of an ethanolic extract of Artemisia dracunculus L. (5011) combined with exercise on in vivo glucose and fat metabolism in diet-induced obese male mice.
33105388	0	43	theme	Diet-induced	96:107	arg1	Mice					115:118	Diet-induced Obese Mice	96:118	Diet-induced Obese Mice	96:118	An Ethanolic Extract of Artemisia dracunculus L. Enhances the Metabolic Benefits of Exercise in Diet-induced Obese Mice.
33105388	1	44	theme	male	325:328	arg1	mice					330:333	diet-induced obese male mice	306:333	diet-induced obese male mice	306:333	PURPOSE The purpose of this study was to determine the effect of an ethanolic extract of Artemisia dracunculus L. (5011) combined with exercise on in vivo glucose and fat metabolism in diet-induced obese male mice.
33105388	8	45	theme	mice	1567:1570	arg1	liver					1539:1543	the liver	1535:1543	the liver of HFD Ex and 5011 Ex mice	1535:1570	5011 Ex mice had increased Pgc1-α and Tfam expression in skeletal muscle when compared with HFD Ex mice, whereas Pdk4 expression was reduced in the liver of HFD Ex and 5011 Ex mice.
33105388	7	46	theme	HFD	1145:1147	arg1	mice					1164:1167	Both HFD Ex and 5011 Ex mice	1140:1167	Both HFD Ex and 5011 Ex mice	1140:1167	Both HFD Ex and 5011 Ex mice had reduced endogenous glucose during the glucose tolerance test, whereas only the 5011 Sed and the 5011 Ex mice had improved glucose disposal after the 4-wk experimental period when compared with HFD Sed and HFD Ex mice.
33105388	2	47	theme	feeding	378:384	arg1	wk					352:353	8 wk	350:353	8 wk of high-fat diet (HFD) feeding	350:384	METHODS After 8 wk of high-fat diet (HFD) feeding, 52 mice were randomly allocated to a voluntary wheel running group (HFD Ex), a 5011 + HFD sedentary group (5011 Sed), a 5011 + HFD Ex (5011 Ex), or an HFD sedentary group (HFD Sed) for 4 wk.
33105388	1	48	dep	in	268:269	arg1	vivo					271:274	vivo	271:274	vivo	271:274	PURPOSE The purpose of this study was to determine the effect of an ethanolic extract of Artemisia dracunculus L. (5011) combined with exercise on in vivo glucose and fat metabolism in diet-induced obese male mice.
33105388	9	49	theme	in	1750:1751	arg1	fat					1758:1760	in vivo fat	1750:1760	in vivo fat	1750:1760	CONCLUSIONS Our study demonstrates that 5011, an ethanolic extract of A. dracunculus L., with a history of medicinal use, enhances the metabolic benefits of exercise to improve in vivo fat and glucose metabolism.
33105388	5	50	from	response	927:934	arg1	liver					996:1000	liver	996:1000	liver	996:1000	We also performed an analysis of genes and proteins associated with the early response to exercise and exercise adaptations in skeletal muscle and liver.
33105388	5	50	from	response	927:934	arg1	muscle					985:990	skeletal muscle	976:990	skeletal muscle	976:990	We also performed an analysis of genes and proteins associated with the early response to exercise and exercise adaptations in skeletal muscle and liver.
33105388	2	51	theme	diet	367:370	arg1	feeding					378:384	high-fat diet (HFD) feeding	358:384	high-fat diet (HFD) feeding	358:384	METHODS After 8 wk of high-fat diet (HFD) feeding, 52 mice were randomly allocated to a voluntary wheel running group (HFD Ex), a 5011 + HFD sedentary group (5011 Sed), a 5011 + HFD Ex (5011 Ex), or an HFD sedentary group (HFD Sed) for 4 wk.
33105388	6	52	contain	had	1056:1058	arg2	oxidation					1074:1082	increased fat oxidation	1060:1082	increased fat oxidation	1060:1082	RESULTS When compared with HFD Ex mice, 5011 Ex mice had increased fat oxidation during speed- and distance-matched wheel running bouts.
33105388	6	52	contain	had	1056:1058	arg1	mice					1051:1054	5011 Ex mice	1043:1054	5011 Ex mice	1043:1054	RESULTS When compared with HFD Ex mice, 5011 Ex mice had increased fat oxidation during speed- and distance-matched wheel running bouts.
33105388	5	53	dep	exercise	939:946	arg1	adaptations					961:971	adaptations	961:971	adaptations	961:971	We also performed an analysis of genes and proteins associated with the early response to exercise and exercise adaptations in skeletal muscle and liver.
33105388	9	54	theme	glucose	1766:1772	arg1	metabolism					1774:1783	glucose metabolism	1766:1783	glucose metabolism	1766:1783	CONCLUSIONS Our study demonstrates that 5011, an ethanolic extract of A. dracunculus L., with a history of medicinal use, enhances the metabolic benefits of exercise to improve in vivo fat and glucose metabolism.
33105388	0	55	theme	Artemisia	24:32	arg1	Extract					13:19	An Ethanolic Extract	0:19	An Ethanolic Extract of Artemisia dracunculus L.	0:47	An Ethanolic Extract of Artemisia dracunculus L. Enhances the Metabolic Benefits of Exercise in Diet-induced Obese Mice.
33105388	4	56	theme	glucose	831:837	arg1	disposal					839:846	glucose disposal	831:846	glucose disposal	831:846	A stable isotope glucose tolerance test was performed before and after the 4-wk wheel running period to determine changes in endogenous glucose production and glucose disposal.
33105388	5	57	theme	skeletal	976:983	arg1	muscle					985:990	skeletal muscle	976:990	skeletal muscle	976:990	We also performed an analysis of genes and proteins associated with the early response to exercise and exercise adaptations in skeletal muscle and liver.
33105388	7	58	theme	improved	1286:1293	arg1	disposal					1303:1310	improved glucose disposal	1286:1310	improved glucose disposal	1286:1310	Both HFD Ex and 5011 Ex mice had reduced endogenous glucose during the glucose tolerance test, whereas only the 5011 Sed and the 5011 Ex mice had improved glucose disposal after the 4-wk experimental period when compared with HFD Sed and HFD Ex mice.
33105388	0	59	dep	Artemisia	24:32	arg1	L.					46:47	Artemisia dracunculus L.	24:47	Artemisia dracunculus L.	24:47	An Ethanolic Extract of Artemisia dracunculus L. Enhances the Metabolic Benefits of Exercise in Diet-induced Obese Mice.
33105388	2	60	dep	METHODS	336:342	arg1	allocated					409:417	allocated	409:417	were randomly allocated to a voluntary wheel running group (HFD Ex), a 5011 + HFD sedentary group (5011 Sed), a 5011 + HFD Ex (5011 Ex), or an HFD sedentary group (HFD Sed) for 4 wk	395:575	METHODS After 8 wk of high-fat diet (HFD) feeding, 52 mice were randomly allocated to a voluntary wheel running group (HFD Ex), a 5011 + HFD sedentary group (5011 Sed), a 5011 + HFD Ex (5011 Ex), or an HFD sedentary group (HFD Sed) for 4 wk.
33105388	8	61	theme	increased	1408:1416	arg1	expression					1434:1443	increased Pgc1-α and Tfam expression	1408:1443	increased Pgc1-α and Tfam expression in skeletal muscle	1408:1462	5011 Ex mice had increased Pgc1-α and Tfam expression in skeletal muscle when compared with HFD Ex mice, whereas Pdk4 expression was reduced in the liver of HFD Ex and 5011 Ex mice.
33105388	1	62	theme	extract	199:205	arg1	effect					176:181	the effect	172:181	the effect of an ethanolic extract of Artemisia dracunculus L	172:232	PURPOSE The purpose of this study was to determine the effect of an ethanolic extract of Artemisia dracunculus L. (5011) combined with exercise on in vivo glucose and fat metabolism in diet-induced obese male mice.
33105388	9	63	dep	CONCLUSIONS	1573:1583	arg1	demonstrates					1595:1606	demonstrates	1595:1606	demonstrates that 5011, an ethanolic extract of A. dracunculus L., with a history of medicinal use, enhances the metabolic benefits of exercise to improve in vivo fat and glucose metabolism	1595:1783	CONCLUSIONS Our study demonstrates that 5011, an ethanolic extract of A. dracunculus L., with a history of medicinal use, enhances the metabolic benefits of exercise to improve in vivo fat and glucose metabolism.
33105388	4	64	from	changes	786:792	arg1	production					816:825	endogenous glucose production	797:825	endogenous glucose production	797:825	A stable isotope glucose tolerance test was performed before and after the 4-wk wheel running period to determine changes in endogenous glucose production and glucose disposal.
33105388	4	64	from	changes	786:792	arg1	disposal					839:846	glucose disposal	831:846	glucose disposal	831:846	A stable isotope glucose tolerance test was performed before and after the 4-wk wheel running period to determine changes in endogenous glucose production and glucose disposal.
33105388	6	65	theme	speed-	1091:1096	arg1	bouts					1133:1137	speed- and distance-matched wheel running bouts	1091:1137	speed- and distance-matched wheel running bouts	1091:1137	RESULTS When compared with HFD Ex mice, 5011 Ex mice had increased fat oxidation during speed- and distance-matched wheel running bouts.
33105388	1	66	theme	L	232:232	arg1	extract					199:205	an ethanolic extract	186:205	an ethanolic extract of Artemisia dracunculus L	186:232	PURPOSE The purpose of this study was to determine the effect of an ethanolic extract of Artemisia dracunculus L. (5011) combined with exercise on in vivo glucose and fat metabolism in diet-induced obese male mice.
33105388	4	67	theme	stable	674:679	arg1	test					707:710	A stable isotope glucose tolerance test	672:710	A stable isotope glucose tolerance test	672:710	A stable isotope glucose tolerance test was performed before and after the 4-wk wheel running period to determine changes in endogenous glucose production and glucose disposal.
33105388	8	68	theme	5011	1391:1394	arg1	mice					1399:1402	5011 Ex mice	1391:1402	5011 Ex mice	1391:1402	5011 Ex mice had increased Pgc1-α and Tfam expression in skeletal muscle when compared with HFD Ex mice, whereas Pdk4 expression was reduced in the liver of HFD Ex and 5011 Ex mice.
33105388	8	69	theme	skeletal	1448:1455	arg1	muscle					1457:1462	skeletal muscle	1448:1462	skeletal muscle	1448:1462	5011 Ex mice had increased Pgc1-α and Tfam expression in skeletal muscle when compared with HFD Ex mice, whereas Pdk4 expression was reduced in the liver of HFD Ex and 5011 Ex mice.
33105388	7	70	theme	5011	1252:1255	arg1	Sed					1257:1259	5011 Sed	1252:1259	5011 Sed	1252:1259	Both HFD Ex and 5011 Ex mice had reduced endogenous glucose during the glucose tolerance test, whereas only the 5011 Sed and the 5011 Ex mice had improved glucose disposal after the 4-wk experimental period when compared with HFD Sed and HFD Ex mice.
33105388	2	71	theme	HFD	473:475	arg1	group					487:491	a 5011 + HFD sedentary group	464:491	group	487:491	METHODS After 8 wk of high-fat diet (HFD) feeding, 52 mice were randomly allocated to a voluntary wheel running group (HFD Ex), a 5011 + HFD sedentary group (5011 Sed), a 5011 + HFD Ex (5011 Ex), or an HFD sedentary group (HFD Sed) for 4 wk.
33105388	6	72	theme	increased	1060:1068	arg1	oxidation					1074:1082	increased fat oxidation	1060:1082	increased fat oxidation	1060:1082	RESULTS When compared with HFD Ex mice, 5011 Ex mice had increased fat oxidation during speed- and distance-matched wheel running bouts.
33105388	7	73	contain	had	1282:1284	arg1	mice					1277:1280	the 5011 Ex mice	1265:1280	the 5011 Ex mice	1265:1280	Both HFD Ex and 5011 Ex mice had reduced endogenous glucose during the glucose tolerance test, whereas only the 5011 Sed and the 5011 Ex mice had improved glucose disposal after the 4-wk experimental period when compared with HFD Sed and HFD Ex mice.
33105388	7	73	contain	had	1282:1284	arg1	Sed					1257:1259	5011 Sed	1252:1259	5011 Sed	1252:1259	Both HFD Ex and 5011 Ex mice had reduced endogenous glucose during the glucose tolerance test, whereas only the 5011 Sed and the 5011 Ex mice had improved glucose disposal after the 4-wk experimental period when compared with HFD Sed and HFD Ex mice.
33105388	7	73	contain	had	1282:1284	arg2	disposal					1303:1310	improved glucose disposal	1286:1310	improved glucose disposal	1286:1310	Both HFD Ex and 5011 Ex mice had reduced endogenous glucose during the glucose tolerance test, whereas only the 5011 Sed and the 5011 Ex mice had improved glucose disposal after the 4-wk experimental period when compared with HFD Sed and HFD Ex mice.
33105388	9	74	theme	A.	1643:1644	arg1	extract					1632:1638	an ethanolic extract	1619:1638	an ethanolic extract of A. dracunculus L., with a history of medicinal use	1619:1692	CONCLUSIONS Our study demonstrates that 5011, an ethanolic extract of A. dracunculus L., with a history of medicinal use, enhances the metabolic benefits of exercise to improve in vivo fat and glucose metabolism.
33105388	9	74	theme	A.	1643:1644	arg1	5011					1613:1616	5011	1613:1616	5011	1613:1616	CONCLUSIONS Our study demonstrates that 5011, an ethanolic extract of A. dracunculus L., with a history of medicinal use, enhances the metabolic benefits of exercise to improve in vivo fat and glucose metabolism.
33105388	7	75	theme	glucose	1211:1217	arg1	test					1229:1232	the glucose tolerance test	1207:1232	the glucose tolerance test	1207:1232	Both HFD Ex and 5011 Ex mice had reduced endogenous glucose during the glucose tolerance test, whereas only the 5011 Sed and the 5011 Ex mice had improved glucose disposal after the 4-wk experimental period when compared with HFD Sed and HFD Ex mice.
33105388	8	76	theme	Ex	1487:1488	arg1	mice					1490:1493	HFD Ex mice	1483:1493	HFD Ex mice	1483:1493	5011 Ex mice had increased Pgc1-α and Tfam expression in skeletal muscle when compared with HFD Ex mice, whereas Pdk4 expression was reduced in the liver of HFD Ex and 5011 Ex mice.
33105388	7	77	theme	endogenous	1181:1190	arg1	glucose					1192:1198	reduced endogenous glucose	1173:1198	reduced endogenous glucose	1173:1198	Both HFD Ex and 5011 Ex mice had reduced endogenous glucose during the glucose tolerance test, whereas only the 5011 Sed and the 5011 Ex mice had improved glucose disposal after the 4-wk experimental period when compared with HFD Sed and HFD Ex mice.
33105388	3	78	theme	Real-time	578:586	arg1	expenditure					595:605	Real-time energy expenditure	578:605	Real-time energy expenditure	578:605	Real-time energy expenditure and substrate utilization were measured by indirect calorimetry.
33105388	6	79	theme	running	1125:1131	arg1	bouts					1133:1137	speed- and distance-matched wheel running bouts	1091:1137	speed- and distance-matched wheel running bouts	1091:1137	RESULTS When compared with HFD Ex mice, 5011 Ex mice had increased fat oxidation during speed- and distance-matched wheel running bouts.
33105388	8	80	theme	Pdk4	1504:1507	arg1	expression					1509:1518	Pdk4 expression	1504:1518	Pdk4 expression	1504:1518	5011 Ex mice had increased Pgc1-α and Tfam expression in skeletal muscle when compared with HFD Ex mice, whereas Pdk4 expression was reduced in the liver of HFD Ex and 5011 Ex mice.
33105388	6	81	theme	Ex	1034:1035	arg1	mice					1037:1040	HFD Ex mice	1030:1040	HFD Ex mice	1030:1040	RESULTS When compared with HFD Ex mice, 5011 Ex mice had increased fat oxidation during speed- and distance-matched wheel running bouts.
33105388	9	82	theme	medicinal	1680:1688	arg1	use					1690:1692	medicinal use	1680:1692	medicinal use	1680:1692	CONCLUSIONS Our study demonstrates that 5011, an ethanolic extract of A. dracunculus L., with a history of medicinal use, enhances the metabolic benefits of exercise to improve in vivo fat and glucose metabolism.
33105388	6	83	theme	distance-matched	1102:1117	arg1	bouts					1133:1137	speed- and distance-matched wheel running bouts	1091:1137	speed- and distance-matched wheel running bouts	1091:1137	RESULTS When compared with HFD Ex mice, 5011 Ex mice had increased fat oxidation during speed- and distance-matched wheel running bouts.
33105388	7	84	theme	Sed	1370:1372	arg1	mice					1385:1388	HFD Sed and HFD Ex mice	1366:1388	mice	1385:1388	Both HFD Ex and 5011 Ex mice had reduced endogenous glucose during the glucose tolerance test, whereas only the 5011 Sed and the 5011 Ex mice had improved glucose disposal after the 4-wk experimental period when compared with HFD Sed and HFD Ex mice.
33105388	7	85	theme	Ex	1161:1162	arg1	mice					1164:1167	Both HFD Ex and 5011 Ex mice	1140:1167	Both HFD Ex and 5011 Ex mice	1140:1167	Both HFD Ex and 5011 Ex mice had reduced endogenous glucose during the glucose tolerance test, whereas only the 5011 Sed and the 5011 Ex mice had improved glucose disposal after the 4-wk experimental period when compared with HFD Sed and HFD Ex mice.
33105388	4	86	theme	glucose	689:695	arg1	test					707:710	A stable isotope glucose tolerance test	672:710	A stable isotope glucose tolerance test	672:710	A stable isotope glucose tolerance test was performed before and after the 4-wk wheel running period to determine changes in endogenous glucose production and glucose disposal.
33105388	3	87	theme	substrate	611:619	arg1	utilization					621:631	substrate utilization	611:631	substrate utilization	611:631	Real-time energy expenditure and substrate utilization were measured by indirect calorimetry.
33105388	0	88	theme	Metabolic	62:70	arg1	Benefits					72:79	the Metabolic Benefits	58:79	the Metabolic Benefits of Exercise in Diet-induced Obese Mice	58:118	An Ethanolic Extract of Artemisia dracunculus L. Enhances the Metabolic Benefits of Exercise in Diet-induced Obese Mice.
33105388	2	89	theme	sedentary	542:550	arg1	group					552:556	an HFD sedentary group	535:556	an HFD sedentary group (HFD Sed)	535:566	METHODS After 8 wk of high-fat diet (HFD) feeding, 52 mice were randomly allocated to a voluntary wheel running group (HFD Ex), a 5011 + HFD sedentary group (5011 Sed), a 5011 + HFD Ex (5011 Ex), or an HFD sedentary group (HFD Sed) for 4 wk.
33105388	2	89	theme	sedentary	542:550	arg1	Sed					563:565	HFD Sed	559:565	HFD Sed	559:565	METHODS After 8 wk of high-fat diet (HFD) feeding, 52 mice were randomly allocated to a voluntary wheel running group (HFD Ex), a 5011 + HFD sedentary group (5011 Sed), a 5011 + HFD Ex (5011 Ex), or an HFD sedentary group (HFD Sed) for 4 wk.
33105388	1	90	theme	fat	288:290	arg1	metabolism					292:301	fat metabolism	288:301	fat metabolism	288:301	PURPOSE The purpose of this study was to determine the effect of an ethanolic extract of Artemisia dracunculus L. (5011) combined with exercise on in vivo glucose and fat metabolism in diet-induced obese male mice.
33105388	8	91	theme	HFD	1548:1550	arg1	mice					1567:1570	HFD Ex and 5011 Ex mice	1548:1570	HFD Ex and 5011 Ex mice	1548:1570	5011 Ex mice had increased Pgc1-α and Tfam expression in skeletal muscle when compared with HFD Ex mice, whereas Pdk4 expression was reduced in the liver of HFD Ex and 5011 Ex mice.
33105388	7	92	theme	experimental	1327:1338	arg1	period					1340:1345	the 4-wk experimental period	1318:1345	the 4-wk experimental period	1318:1345	Both HFD Ex and 5011 Ex mice had reduced endogenous glucose during the glucose tolerance test, whereas only the 5011 Sed and the 5011 Ex mice had improved glucose disposal after the 4-wk experimental period when compared with HFD Sed and HFD Ex mice.
33105388	4	93	theme	wheel	752:756	arg1	running					758:764	the 4-wk wheel running	743:764	the 4-wk wheel running period	743:771	A stable isotope glucose tolerance test was performed before and after the 4-wk wheel running period to determine changes in endogenous glucose production and glucose disposal.
33105388	9	94	theme	exercise	1730:1737	arg1	benefits					1718:1725	the metabolic benefits	1704:1725	the metabolic benefits of exercise	1704:1737	CONCLUSIONS Our study demonstrates that 5011, an ethanolic extract of A. dracunculus L., with a history of medicinal use, enhances the metabolic benefits of exercise to improve in vivo fat and glucose metabolism.
33105388	0	95	theme	Obese	109:113	arg1	Mice					115:118	Diet-induced Obese Mice	96:118	Diet-induced Obese Mice	96:118	An Ethanolic Extract of Artemisia dracunculus L. Enhances the Metabolic Benefits of Exercise in Diet-induced Obese Mice.
33105388	1	96	theme	obese	319:323	arg1	mice					330:333	diet-induced obese male mice	306:333	diet-induced obese male mice	306:333	PURPOSE The purpose of this study was to determine the effect of an ethanolic extract of Artemisia dracunculus L. (5011) combined with exercise on in vivo glucose and fat metabolism in diet-induced obese male mice.
33105388	2	97	theme	voluntary	424:432	arg1	wheel					434:438	a voluntary wheel	422:438	a voluntary wheel running group (HFD Ex), a 5011 + HFD sedentary group (5011 Sed), a 5011 + HFD Ex (5011 Ex), or an HFD sedentary group (HFD Sed)	422:566	METHODS After 8 wk of high-fat diet (HFD) feeding, 52 mice were randomly allocated to a voluntary wheel running group (HFD Ex), a 5011 + HFD sedentary group (5011 Sed), a 5011 + HFD Ex (5011 Ex), or an HFD sedentary group (HFD Sed) for 4 wk.
33105388	0	98	theme	Ethanolic	3:11	arg1	Extract					13:19	An Ethanolic Extract	0:19	An Ethanolic Extract of Artemisia dracunculus L.	0:47	An Ethanolic Extract of Artemisia dracunculus L. Enhances the Metabolic Benefits of Exercise in Diet-induced Obese Mice.
33105388	8	99	theme	Ex	1564:1565	arg1	mice					1567:1570	HFD Ex and 5011 Ex mice	1548:1570	HFD Ex and 5011 Ex mice	1548:1570	5011 Ex mice had increased Pgc1-α and Tfam expression in skeletal muscle when compared with HFD Ex mice, whereas Pdk4 expression was reduced in the liver of HFD Ex and 5011 Ex mice.
33105388	8	100	from	expression	1434:1443	arg1	muscle					1457:1462	skeletal muscle	1448:1462	skeletal muscle	1448:1462	5011 Ex mice had increased Pgc1-α and Tfam expression in skeletal muscle when compared with HFD Ex mice, whereas Pdk4 expression was reduced in the liver of HFD Ex and 5011 Ex mice.
33105388	2	101	theme	high-fat	358:365	arg1	HFD					373:375	HFD	373:375	HFD	373:375	METHODS After 8 wk of high-fat diet (HFD) feeding, 52 mice were randomly allocated to a voluntary wheel running group (HFD Ex), a 5011 + HFD sedentary group (5011 Sed), a 5011 + HFD Ex (5011 Ex), or an HFD sedentary group (HFD Sed) for 4 wk.
33105388	2	101	theme	high-fat	358:365	arg1	diet					367:370	high-fat diet	358:370	high-fat diet (HFD) feeding	358:384	METHODS After 8 wk of high-fat diet (HFD) feeding, 52 mice were randomly allocated to a voluntary wheel running group (HFD Ex), a 5011 + HFD sedentary group (5011 Sed), a 5011 + HFD Ex (5011 Ex), or an HFD sedentary group (HFD Sed) for 4 wk.
33105388	1	102	from	metabolism	292:301	arg1	mice					330:333	diet-induced obese male mice	306:333	diet-induced obese male mice	306:333	PURPOSE The purpose of this study was to determine the effect of an ethanolic extract of Artemisia dracunculus L. (5011) combined with exercise on in vivo glucose and fat metabolism in diet-induced obese male mice.
33105388	7	103	theme	4-wk	1322:1325	arg1	period					1340:1345	the 4-wk experimental period	1318:1345	the 4-wk experimental period	1318:1345	Both HFD Ex and 5011 Ex mice had reduced endogenous glucose during the glucose tolerance test, whereas only the 5011 Sed and the 5011 Ex mice had improved glucose disposal after the 4-wk experimental period when compared with HFD Sed and HFD Ex mice.
33105388	4	104	theme	glucose	808:814	arg1	production					816:825	endogenous glucose production	797:825	endogenous glucose production	797:825	A stable isotope glucose tolerance test was performed before and after the 4-wk wheel running period to determine changes in endogenous glucose production and glucose disposal.
33105388	1	105	theme	Artemisia	210:218	arg1	L					232:232	Artemisia dracunculus L	210:232	Artemisia dracunculus L	210:232	PURPOSE The purpose of this study was to determine the effect of an ethanolic extract of Artemisia dracunculus L. (5011) combined with exercise on in vivo glucose and fat metabolism in diet-induced obese male mice.
33105388	1	106	theme	in	268:269	arg1	glucose					276:282	in vivo glucose	268:282	in vivo glucose	268:282	PURPOSE The purpose of this study was to determine the effect of an ethanolic extract of Artemisia dracunculus L. (5011) combined with exercise on in vivo glucose and fat metabolism in diet-induced obese male mice.
33105388	5	107	theme	early	921:925	arg1	response					927:934	the early response	917:934	the early response to exercise and exercise adaptations in skeletal muscle and liver	917:1000	We also performed an analysis of genes and proteins associated with the early response to exercise and exercise adaptations in skeletal muscle and liver.
33105388	8	108	theme	Pgc1-α	1418:1423	arg1	expression					1434:1443	increased Pgc1-α and Tfam expression	1408:1443	increased Pgc1-α and Tfam expression in skeletal muscle	1408:1462	5011 Ex mice had increased Pgc1-α and Tfam expression in skeletal muscle when compared with HFD Ex mice, whereas Pdk4 expression was reduced in the liver of HFD Ex and 5011 Ex mice.
33105388	7	109	dep	Sed	1257:1259	arg1	the					1248:1250	the	1248:1250	the	1248:1250	Both HFD Ex and 5011 Ex mice had reduced endogenous glucose during the glucose tolerance test, whereas only the 5011 Sed and the 5011 Ex mice had improved glucose disposal after the 4-wk experimental period when compared with HFD Sed and HFD Ex mice.
33105388	7	110	theme	glucose	1295:1301	arg1	disposal					1303:1310	improved glucose disposal	1286:1310	improved glucose disposal	1286:1310	Both HFD Ex and 5011 Ex mice had reduced endogenous glucose during the glucose tolerance test, whereas only the 5011 Sed and the 5011 Ex mice had improved glucose disposal after the 4-wk experimental period when compared with HFD Sed and HFD Ex mice.
33105388	8	111	theme	Tfam	1429:1432	arg1	expression					1434:1443	increased Pgc1-α and Tfam expression	1408:1443	increased Pgc1-α and Tfam expression in skeletal muscle	1408:1462	5011 Ex mice had increased Pgc1-α and Tfam expression in skeletal muscle when compared with HFD Ex mice, whereas Pdk4 expression was reduced in the liver of HFD Ex and 5011 Ex mice.
33105388	1	112	theme	dracunculus	220:230	arg1	L					232:232	Artemisia dracunculus L	210:232	Artemisia dracunculus L	210:232	PURPOSE The purpose of this study was to determine the effect of an ethanolic extract of Artemisia dracunculus L. (5011) combined with exercise on in vivo glucose and fat metabolism in diet-induced obese male mice.
31697228	7	0	theme	strain	827:832	arg1	Seoho-28T					834:842	the strain Seoho-28T	823:842	the strain Seoho-28T	823:842	The genomic DNA G+C content of the strain Seoho-28T was 74.2 mol%.
31697228	8	1	with	comparisons	867:877	arg1	families					904:911	families	904:911	families in the order Solirubrobacterales	904:944	Genomic comparisons of strain Seoho-28T with families in the order Solirubrobacterales were made using the Genome-to-Genome Distance Calculator, average nucleotide identity and average amino acid identity analyses (values indicated ≤14.9, ≤73.5 and ≤57.8 %, respectively).
31697228	8	2	theme	Distance	983:990	arg1	Calculator					992:1001	the Genome-to-Genome Distance Calculator	962:1001	the Genome-to-Genome Distance Calculator	962:1001	Genomic comparisons of strain Seoho-28T with families in the order Solirubrobacterales were made using the Genome-to-Genome Distance Calculator, average nucleotide identity and average amino acid identity analyses (values indicated ≤14.9, ≤73.5 and ≤57.8 %, respectively).
31697228	8	2	theme	Distance	983:990	arg1	analyses					1064:1071	average nucleotide identity and average amino acid identity analyses	1004:1071	average nucleotide identity and average amino acid identity analyses (values indicated ≤14.9, ≤73.5 and ≤57.8 %, respectively)	1004:1129	Genomic comparisons of strain Seoho-28T with families in the order Solirubrobacterales were made using the Genome-to-Genome Distance Calculator, average nucleotide identity and average amino acid identity analyses (values indicated ≤14.9, ≤73.5 and ≤57.8 %, respectively).
31697228	9	3	theme	 1	1177:1178	arg1	ω9c					1180:1182	 1 ω9c	1177:1182	C16 : 0-iso, C18 : 1 ω9c	1159:1182	Strain Seoho-28T contained C16 : 0-iso, C18 : 1 ω9c and C16 : 0 as major fatty acids and MK-7 (H4) as the major quinone.
31697228	7	4	theme	G+C	808:810	arg1	%					856:856	74.2 mol%	848:856	74.2 mol%	848:856	The genomic DNA G+C content of the strain Seoho-28T was 74.2 mol%.
31697228	7	4	theme	G+C	808:810	arg1	content					812:818	The genomic DNA G+C content	792:818	The genomic DNA G+C content of the strain Seoho-28T	792:842	The genomic DNA G+C content of the strain Seoho-28T was 74.2 mol%.
31697228	7	5	theme	genomic	796:802	arg1	%					856:856	74.2 mol%	848:856	74.2 mol%	848:856	The genomic DNA G+C content of the strain Seoho-28T was 74.2 mol%.
31697228	7	5	theme	genomic	796:802	arg1	content					812:818	The genomic DNA G+C content	792:818	The genomic DNA G+C content of the strain Seoho-28T	792:842	The genomic DNA G+C content of the strain Seoho-28T was 74.2 mol%.
31697228	1	6	theme	Paraconexibacteraceae	173:193	arg1	fam					195:197	Paraconexibacteraceae fam	173:197	Paraconexibacteraceae fam	173:197	nov., a novel actinobacterium isolated from a eutrophic lake during the end of cyanobacterial harmful algal blooms, and proposal of Paraconexibacteraceae fam.
31697228	5	7	theme	%	554:554	arg1	NaCl					556:559	0 % NaCl	552:559	0 % NaCl	552:559	The strain grew optimally with 0 % NaCl and at 25-30 °C on Reasoner's 2A medium.
31697228	6	8	theme	16S	637:639	arg1	sequences					651:659	16S rRNA gene sequences	637:659	16S rRNA gene sequences	637:659	The phylogenetic analysis based on 16S rRNA gene sequences positioned the novel strain among the order Solirubrobacterales, but sequence similarities to known species were less than 94.7 %.
31697228	9	9	theme	fatty	1205:1209	arg1	C16 					1159:1162	C16 	1159:1162	C16 	1159:1162	Strain Seoho-28T contained C16 : 0-iso, C18 : 1 ω9c and C16 : 0 as major fatty acids and MK-7 (H4) as the major quinone.
31697228	9	9	theme	fatty	1205:1209	arg1	C16 					1188:1191	C16 	1188:1191	C16 	1188:1191	Strain Seoho-28T contained C16 : 0-iso, C18 : 1 ω9c and C16 : 0 as major fatty acids and MK-7 (H4) as the major quinone.
31697228	9	9	theme	fatty	1205:1209	arg1	acids					1211:1215	major fatty acids	1199:1215	major fatty acids	1199:1215	Strain Seoho-28T contained C16 : 0-iso, C18 : 1 ω9c and C16 : 0 as major fatty acids and MK-7 (H4) as the major quinone.
31697228	9	10	dep	C16 	1159:1162	arg1	C18 					1172:1175	C18 	1172:1175	C18 	1172:1175	Strain Seoho-28T contained C16 : 0-iso, C18 : 1 ω9c and C16 : 0 as major fatty acids and MK-7 (H4) as the major quinone.
31697228	9	10	dep	C16 	1159:1162	arg1	ω9c					1180:1182	 1 ω9c	1177:1182	C16 : 0-iso, C18 : 1 ω9c	1159:1182	Strain Seoho-28T contained C16 : 0-iso, C18 : 1 ω9c and C16 : 0 as major fatty acids and MK-7 (H4) as the major quinone.
31697228	12	11	theme	chemotaxonomic	1505:1518	arg1	results					1535:1541	the genotypic, chemotaxonomic and phenotypic results	1490:1541	the genotypic, chemotaxonomic and phenotypic results	1490:1541	Based on the genotypic, chemotaxonomic and phenotypic results, strain Seoho-28T represents a novel genus and species, Paraconexibacter algicola gen. nov., sp.
31697228	3	12	theme	eutrophic	304:312	arg1	lake					314:317	a shallow eutrophic lake	294:317	a shallow eutrophic lake	294:317	A novel bacterium, strain Seoho-28T, was isolated from a shallow eutrophic lake during the end of cyanobacterial harmful algal blooms and was characterized taxonomically and phylogenetically.
31697228	1	13	theme	cyanobacterial	120:133	arg1	blooms					149:154	cyanobacterial harmful algal blooms	120:154	cyanobacterial harmful algal blooms	120:154	nov., a novel actinobacterium isolated from a eutrophic lake during the end of cyanobacterial harmful algal blooms, and proposal of Paraconexibacteraceae fam.
31697228	12	14	theme	phenotypic	1524:1533	arg1	results					1535:1541	the genotypic, chemotaxonomic and phenotypic results	1490:1541	the genotypic, chemotaxonomic and phenotypic results	1490:1541	Based on the genotypic, chemotaxonomic and phenotypic results, strain Seoho-28T represents a novel genus and species, Paraconexibacter algicola gen. nov., sp.
31697228	6	15	theme	phylogenetic	606:617	arg1	analysis					619:626	The phylogenetic analysis	602:626	The phylogenetic analysis based on 16S rRNA gene sequences	602:659	The phylogenetic analysis based on 16S rRNA gene sequences positioned the novel strain among the order Solirubrobacterales, but sequence similarities to known species were less than 94.7 %.
31697228	8	16	theme	nucleotide	1012:1021	arg1	identity					1023:1030	average nucleotide identity	1004:1030	average nucleotide identity	1004:1030	Genomic comparisons of strain Seoho-28T with families in the order Solirubrobacterales were made using the Genome-to-Genome Distance Calculator, average nucleotide identity and average amino acid identity analyses (values indicated ≤14.9, ≤73.5 and ≤57.8 %, respectively).
31697228	1	17	theme	algal	143:147	arg1	blooms					149:154	cyanobacterial harmful algal blooms	120:154	cyanobacterial harmful algal blooms	120:154	nov., a novel actinobacterium isolated from a eutrophic lake during the end of cyanobacterial harmful algal blooms, and proposal of Paraconexibacteraceae fam.
31697228	5	18	theme	2A	591:592	arg1	medium					594:599	Reasoner's 2A medium	580:599	Reasoner's 2A medium	580:599	The strain grew optimally with 0 % NaCl and at 25-30 °C on Reasoner's 2A medium.
31697228	12	19	theme	algicola	1616:1623	arg1	nov.					1630:1633	Paraconexibacter algicola gen. nov.	1599:1633	Paraconexibacter algicola gen. nov.	1599:1633	Based on the genotypic, chemotaxonomic and phenotypic results, strain Seoho-28T represents a novel genus and species, Paraconexibacter algicola gen. nov., sp.
31697228	12	19	theme	algicola	1616:1623	arg1	genus					1580:1584	a novel genus	1572:1584	a novel genus	1572:1584	Based on the genotypic, chemotaxonomic and phenotypic results, strain Seoho-28T represents a novel genus and species, Paraconexibacter algicola gen. nov., sp.
31697228	12	19	theme	algicola	1616:1623	arg1	sp					1636:1637	sp	1636:1637	sp	1636:1637	Based on the genotypic, chemotaxonomic and phenotypic results, strain Seoho-28T represents a novel genus and species, Paraconexibacter algicola gen. nov., sp.
31697228	12	20	theme	genotypic	1494:1502	arg1	results					1535:1541	the genotypic, chemotaxonomic and phenotypic results	1490:1541	the genotypic, chemotaxonomic and phenotypic results	1490:1541	Based on the genotypic, chemotaxonomic and phenotypic results, strain Seoho-28T represents a novel genus and species, Paraconexibacter algicola gen. nov., sp.
31697228	11	21	theme	ll-diaminopimelic	1391:1407	arg1	acids					1443:1447	the diagnostic diamino acids	1420:1447	the diagnostic diamino acids in the cell-wall peptidoglycan	1420:1478	Meso- and ll-diaminopimelic acids were the diagnostic diamino acids in the cell-wall peptidoglycan.
31697228	11	21	theme	ll-diaminopimelic	1391:1407	arg1	acids					1409:1413	ll-diaminopimelic acids	1391:1413	ll-diaminopimelic acids	1391:1413	Meso- and ll-diaminopimelic acids were the diagnostic diamino acids in the cell-wall peptidoglycan.
31697228	11	21	theme	ll-diaminopimelic	1391:1407	arg1	Meso-					1381:1385	Meso-	1381:1385	Meso-	1381:1385	Meso- and ll-diaminopimelic acids were the diagnostic diamino acids in the cell-wall peptidoglycan.
31697228	8	22	theme	amino	1044:1048	arg1	identity					1055:1062	average amino acid identity	1036:1062	average amino acid identity	1036:1062	Genomic comparisons of strain Seoho-28T with families in the order Solirubrobacterales were made using the Genome-to-Genome Distance Calculator, average nucleotide identity and average amino acid identity analyses (values indicated ≤14.9, ≤73.5 and ≤57.8 %, respectively).
31697228	8	23	from	families	904:911	arg1	Solirubrobacterales					926:944	the order Solirubrobacterales	916:944	the order Solirubrobacterales	916:944	Genomic comparisons of strain Seoho-28T with families in the order Solirubrobacterales were made using the Genome-to-Genome Distance Calculator, average nucleotide identity and average amino acid identity analyses (values indicated ≤14.9, ≤73.5 and ≤57.8 %, respectively).
31697228	3	24	theme	algal	360:364	arg1	blooms					366:371	cyanobacterial harmful algal blooms	337:371	cyanobacterial harmful algal blooms	337:371	A novel bacterium, strain Seoho-28T, was isolated from a shallow eutrophic lake during the end of cyanobacterial harmful algal blooms and was characterized taxonomically and phylogenetically.
31697228	6	25	theme	novel	676:680	arg1	strain					682:687	the novel strain	672:687	the novel strain among the order Solirubrobacterales	672:723	The phylogenetic analysis based on 16S rRNA gene sequences positioned the novel strain among the order Solirubrobacterales, but sequence similarities to known species were less than 94.7 %.
31697228	0	26	theme	Paraconexibacter	0:15	arg1	nov.					31:34	Paraconexibacter algicola gen. nov.	0:34	Paraconexibacter algicola gen. nov.	0:34	Paraconexibacter algicola gen. nov., sp.
31697228	8	27	theme	identity	1055:1062	arg1	Calculator					992:1001	the Genome-to-Genome Distance Calculator	962:1001	the Genome-to-Genome Distance Calculator	962:1001	Genomic comparisons of strain Seoho-28T with families in the order Solirubrobacterales were made using the Genome-to-Genome Distance Calculator, average nucleotide identity and average amino acid identity analyses (values indicated ≤14.9, ≤73.5 and ≤57.8 %, respectively).
31697228	8	27	theme	identity	1055:1062	arg1	analyses					1064:1071	average nucleotide identity and average amino acid identity analyses	1004:1071	average nucleotide identity and average amino acid identity analyses (values indicated ≤14.9, ≤73.5 and ≤57.8 %, respectively)	1004:1129	Genomic comparisons of strain Seoho-28T with families in the order Solirubrobacterales were made using the Genome-to-Genome Distance Calculator, average nucleotide identity and average amino acid identity analyses (values indicated ≤14.9, ≤73.5 and ≤57.8 %, respectively).
31697228	11	28	from	acids	1443:1447	arg1	peptidoglycan					1466:1478	the cell-wall peptidoglycan	1452:1478	the cell-wall peptidoglycan	1452:1478	Meso- and ll-diaminopimelic acids were the diagnostic diamino acids in the cell-wall peptidoglycan.
31697228	3	29	theme	cyanobacterial	337:350	arg1	blooms					366:371	cyanobacterial harmful algal blooms	337:371	cyanobacterial harmful algal blooms	337:371	A novel bacterium, strain Seoho-28T, was isolated from a shallow eutrophic lake during the end of cyanobacterial harmful algal blooms and was characterized taxonomically and phylogenetically.
31697228	3	30	attach	isolated	280:287	arg2	bacterium					247:255	A novel bacterium	239:255	A novel bacterium	239:255	A novel bacterium, strain Seoho-28T, was isolated from a shallow eutrophic lake during the end of cyanobacterial harmful algal blooms and was characterized taxonomically and phylogenetically.
31697228	3	30	attach	isolated	280:287	arg1	lake					314:317	a shallow eutrophic lake	294:317	a shallow eutrophic lake	294:317	A novel bacterium, strain Seoho-28T, was isolated from a shallow eutrophic lake during the end of cyanobacterial harmful algal blooms and was characterized taxonomically and phylogenetically.
31697228	3	30	attach	isolated	280:287	arg2	strain					258:263	strain Seoho-28T	258:273	strain Seoho-28T	258:273	A novel bacterium, strain Seoho-28T, was isolated from a shallow eutrophic lake during the end of cyanobacterial harmful algal blooms and was characterized taxonomically and phylogenetically.
31697228	11	31	theme	diagnostic	1424:1433	arg1	acids					1443:1447	the diagnostic diamino acids	1420:1447	the diagnostic diamino acids in the cell-wall peptidoglycan	1420:1478	Meso- and ll-diaminopimelic acids were the diagnostic diamino acids in the cell-wall peptidoglycan.
31697228	11	31	theme	diagnostic	1424:1433	arg1	acids					1409:1413	ll-diaminopimelic acids	1391:1413	ll-diaminopimelic acids	1391:1413	Meso- and ll-diaminopimelic acids were the diagnostic diamino acids in the cell-wall peptidoglycan.
31697228	11	31	theme	diagnostic	1424:1433	arg1	Meso-					1381:1385	Meso-	1381:1385	Meso-	1381:1385	Meso- and ll-diaminopimelic acids were the diagnostic diamino acids in the cell-wall peptidoglycan.
31697228	0	32	theme	gen.	26:29	arg1	nov.					31:34	Paraconexibacter algicola gen. nov.	0:34	Paraconexibacter algicola gen. nov.	0:34	Paraconexibacter algicola gen. nov., sp.
31697228	1	33	theme	eutrophic	87:95	arg1	lake					97:100	a eutrophic lake	85:100	a eutrophic lake during the end of cyanobacterial harmful algal blooms	85:154	nov., a novel actinobacterium isolated from a eutrophic lake during the end of cyanobacterial harmful algal blooms, and proposal of Paraconexibacteraceae fam.
31697228	8	34	theme	strain	882:887	arg1	Seoho-28T					889:897	strain Seoho-28T	882:897	strain Seoho-28T	882:897	Genomic comparisons of strain Seoho-28T with families in the order Solirubrobacterales were made using the Genome-to-Genome Distance Calculator, average nucleotide identity and average amino acid identity analyses (values indicated ≤14.9, ≤73.5 and ≤57.8 %, respectively).
31697228	6	35	theme	gene	646:649	arg1	sequences					651:659	16S rRNA gene sequences	637:659	16S rRNA gene sequences	637:659	The phylogenetic analysis based on 16S rRNA gene sequences positioned the novel strain among the order Solirubrobacterales, but sequence similarities to known species were less than 94.7 %.
31697228	10	36	contain	contained	1270:1278	arg2	phosphatidylinositol					1304:1323	phosphatidylinositol	1304:1323	phosphatidylinositol	1304:1323	Strain Seoho-28T contained diphosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid as major polar lipids.
31697228	10	36	contain	contained	1270:1278	arg2	lipids					1373:1378	major polar lipids	1361:1378	major polar lipids	1361:1378	Strain Seoho-28T contained diphosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid as major polar lipids.
31697228	10	36	contain	contained	1270:1278	arg2	diphosphatidylglycerol					1280:1301	diphosphatidylglycerol	1280:1301	diphosphatidylglycerol	1280:1301	Strain Seoho-28T contained diphosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid as major polar lipids.
31697228	10	36	contain	contained	1270:1278	arg1	Seoho-28T					1260:1268	Strain Seoho-28T	1253:1268	Strain Seoho-28T	1253:1268	Strain Seoho-28T contained diphosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid as major polar lipids.
31697228	10	36	contain	contained	1270:1278	arg2	phospholipid					1345:1356	an unidentified phospholipid	1329:1356	an unidentified phospholipid	1329:1356	Strain Seoho-28T contained diphosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid as major polar lipids.
31697228	9	37	contain	contained	1149:1157	arg2	acids					1211:1215	major fatty acids	1199:1215	major fatty acids	1199:1215	Strain Seoho-28T contained C16 : 0-iso, C18 : 1 ω9c and C16 : 0 as major fatty acids and MK-7 (H4) as the major quinone.
31697228	9	37	contain	contained	1149:1157	arg2	C16 					1159:1162	C16 	1159:1162	C16 	1159:1162	Strain Seoho-28T contained C16 : 0-iso, C18 : 1 ω9c and C16 : 0 as major fatty acids and MK-7 (H4) as the major quinone.
31697228	9	37	contain	contained	1149:1157	arg1	Seoho-28T					1139:1147	Strain Seoho-28T	1132:1147	Strain Seoho-28T	1132:1147	Strain Seoho-28T contained C16 : 0-iso, C18 : 1 ω9c and C16 : 0 as major fatty acids and MK-7 (H4) as the major quinone.
31697228	9	37	contain	contained	1149:1157	arg2	C16 					1188:1191	C16 	1188:1191	C16 	1188:1191	Strain Seoho-28T contained C16 : 0-iso, C18 : 1 ω9c and C16 : 0 as major fatty acids and MK-7 (H4) as the major quinone.
31697228	9	37	contain	contained	1149:1157	arg2	quinone					1244:1250	the major quinone	1234:1250	the major quinone	1234:1250	Strain Seoho-28T contained C16 : 0-iso, C18 : 1 ω9c and C16 : 0 as major fatty acids and MK-7 (H4) as the major quinone.
31697228	10	38	theme	Strain	1253:1258	arg1	Seoho-28T					1260:1268	Strain Seoho-28T	1253:1268	Strain Seoho-28T	1253:1268	Strain Seoho-28T contained diphosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid as major polar lipids.
31697228	6	39	theme	sequence	730:737	arg1	similarities					739:750	sequence similarities	730:750	sequence similarities to known species	730:767	The phylogenetic analysis based on 16S rRNA gene sequences positioned the novel strain among the order Solirubrobacterales, but sequence similarities to known species were less than 94.7 %.
31697228	8	40	theme	order	920:924	arg1	Solirubrobacterales					926:944	the order Solirubrobacterales	916:944	the order Solirubrobacterales	916:944	Genomic comparisons of strain Seoho-28T with families in the order Solirubrobacterales were made using the Genome-to-Genome Distance Calculator, average nucleotide identity and average amino acid identity analyses (values indicated ≤14.9, ≤73.5 and ≤57.8 %, respectively).
31697228	14	41	theme	39791T=JCM	1798:1807	arg1	31881T					1809:1814	=KCTC 39791T=JCM 31881T	1792:1814	=KCTC 39791T=JCM 31881T	1792:1814	The type strain is Seoho-28T (=KCTC 39791T=JCM 31881T).
31697228	14	41	theme	39791T=JCM	1798:1807	arg1	Seoho-28T					1781:1789	Seoho-28T	1781:1789	Seoho-28T (=KCTC 39791T=JCM 31881T)	1781:1815	The type strain is Seoho-28T (=KCTC 39791T=JCM 31881T).
31697228	8	42	dep	analyses	1064:1071	arg1	values					1074:1079	values	1074:1079	values indicated ≤14.9, ≤73.5 and ≤57.8 %, respectively	1074:1128	Genomic comparisons of strain Seoho-28T with families in the order Solirubrobacterales were made using the Genome-to-Genome Distance Calculator, average nucleotide identity and average amino acid identity analyses (values indicated ≤14.9, ≤73.5 and ≤57.8 %, respectively).
31697228	9	43	theme	Strain	1132:1137	arg1	Seoho-28T					1139:1147	Strain Seoho-28T	1132:1147	Strain Seoho-28T	1132:1147	Strain Seoho-28T contained C16 : 0-iso, C18 : 1 ω9c and C16 : 0 as major fatty acids and MK-7 (H4) as the major quinone.
31697228	6	44	theme	order	699:703	arg1	Solirubrobacterales					705:723	the order Solirubrobacterales	695:723	the order Solirubrobacterales	695:723	The phylogenetic analysis based on 16S rRNA gene sequences positioned the novel strain among the order Solirubrobacterales, but sequence similarities to known species were less than 94.7 %.
31697228	13	45	theme	class	1739:1743	arg1	Thermoleophilia					1745:1759	the class Thermoleophilia	1735:1759	the class Thermoleophilia	1735:1759	nov., which belongs to a new family Paraconexibacteraceae in the order Solirubrobacterales and the class Thermoleophilia.
31697228	10	46	theme	unidentified	1332:1343	arg1	diphosphatidylglycerol					1280:1301	diphosphatidylglycerol	1280:1301	diphosphatidylglycerol	1280:1301	Strain Seoho-28T contained diphosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid as major polar lipids.
31697228	10	46	theme	unidentified	1332:1343	arg1	phospholipid					1345:1356	an unidentified phospholipid	1329:1356	an unidentified phospholipid	1329:1356	Strain Seoho-28T contained diphosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid as major polar lipids.
31697228	10	46	theme	unidentified	1332:1343	arg1	phosphatidylinositol					1304:1323	phosphatidylinositol	1304:1323	phosphatidylinositol	1304:1323	Strain Seoho-28T contained diphosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid as major polar lipids.
31697228	10	46	theme	unidentified	1332:1343	arg1	lipids					1373:1378	major polar lipids	1361:1378	major polar lipids	1361:1378	Strain Seoho-28T contained diphosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid as major polar lipids.
31697228	7	47	theme	74.2 mol	848:855	arg1	%					856:856	74.2 mol%	848:856	74.2 mol%	848:856	The genomic DNA G+C content of the strain Seoho-28T was 74.2 mol%.
31697228	7	47	theme	74.2 mol	848:855	arg1	content					812:818	The genomic DNA G+C content	792:818	The genomic DNA G+C content of the strain Seoho-28T	792:842	The genomic DNA G+C content of the strain Seoho-28T was 74.2 mol%.
31697228	8	48	theme	Genome-to-Genome	966:981	arg1	Calculator					992:1001	the Genome-to-Genome Distance Calculator	962:1001	the Genome-to-Genome Distance Calculator	962:1001	Genomic comparisons of strain Seoho-28T with families in the order Solirubrobacterales were made using the Genome-to-Genome Distance Calculator, average nucleotide identity and average amino acid identity analyses (values indicated ≤14.9, ≤73.5 and ≤57.8 %, respectively).
31697228	8	48	theme	Genome-to-Genome	966:981	arg1	analyses					1064:1071	average nucleotide identity and average amino acid identity analyses	1004:1071	average nucleotide identity and average amino acid identity analyses (values indicated ≤14.9, ≤73.5 and ≤57.8 %, respectively)	1004:1129	Genomic comparisons of strain Seoho-28T with families in the order Solirubrobacterales were made using the Genome-to-Genome Distance Calculator, average nucleotide identity and average amino acid identity analyses (values indicated ≤14.9, ≤73.5 and ≤57.8 %, respectively).
31697228	14	49	theme	=KCTC	1792:1796	arg1	31881T					1809:1814	=KCTC 39791T=JCM 31881T	1792:1814	=KCTC 39791T=JCM 31881T	1792:1814	The type strain is Seoho-28T (=KCTC 39791T=JCM 31881T).
31697228	14	49	theme	=KCTC	1792:1796	arg1	Seoho-28T					1781:1789	Seoho-28T	1781:1789	Seoho-28T (=KCTC 39791T=JCM 31881T)	1781:1815	The type strain is Seoho-28T (=KCTC 39791T=JCM 31881T).
31697228	7	50	theme	Seoho-28T	834:842	arg1	%					856:856	74.2 mol%	848:856	74.2 mol%	848:856	The genomic DNA G+C content of the strain Seoho-28T was 74.2 mol%.
31697228	7	50	theme	Seoho-28T	834:842	arg1	content					812:818	The genomic DNA G+C content	792:818	The genomic DNA G+C content of the strain Seoho-28T	792:842	The genomic DNA G+C content of the strain Seoho-28T was 74.2 mol%.
31697228	1	51	theme	blooms	149:154	arg1	end					113:115	the end	109:115	the end of cyanobacterial harmful algal blooms	109:154	nov., a novel actinobacterium isolated from a eutrophic lake during the end of cyanobacterial harmful algal blooms, and proposal of Paraconexibacteraceae fam.
31697228	3	52	theme	novel	241:245	arg1	strain					258:263	strain Seoho-28T	258:273	strain Seoho-28T	258:273	A novel bacterium, strain Seoho-28T, was isolated from a shallow eutrophic lake during the end of cyanobacterial harmful algal blooms and was characterized taxonomically and phylogenetically.
31697228	3	52	theme	novel	241:245	arg1	bacterium					247:255	A novel bacterium	239:255	A novel bacterium	239:255	A novel bacterium, strain Seoho-28T, was isolated from a shallow eutrophic lake during the end of cyanobacterial harmful algal blooms and was characterized taxonomically and phylogenetically.
31697228	13	53	theme	order	1705:1709	arg1	Solirubrobacterales					1711:1729	the order Solirubrobacterales	1701:1729	the order Solirubrobacterales	1701:1729	nov., which belongs to a new family Paraconexibacteraceae in the order Solirubrobacterales and the class Thermoleophilia.
31697228	10	54	theme	polar	1367:1371	arg1	diphosphatidylglycerol					1280:1301	diphosphatidylglycerol	1280:1301	diphosphatidylglycerol	1280:1301	Strain Seoho-28T contained diphosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid as major polar lipids.
31697228	10	54	theme	polar	1367:1371	arg1	phosphatidylinositol					1304:1323	phosphatidylinositol	1304:1323	phosphatidylinositol	1304:1323	Strain Seoho-28T contained diphosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid as major polar lipids.
31697228	10	54	theme	polar	1367:1371	arg1	phospholipid					1345:1356	an unidentified phospholipid	1329:1356	an unidentified phospholipid	1329:1356	Strain Seoho-28T contained diphosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid as major polar lipids.
31697228	10	54	theme	polar	1367:1371	arg1	lipids					1373:1378	major polar lipids	1361:1378	major polar lipids	1361:1378	Strain Seoho-28T contained diphosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid as major polar lipids.
31697228	12	55	theme	novel	1574:1578	arg1	nov.					1630:1633	Paraconexibacter algicola gen. nov.	1599:1633	Paraconexibacter algicola gen. nov.	1599:1633	Based on the genotypic, chemotaxonomic and phenotypic results, strain Seoho-28T represents a novel genus and species, Paraconexibacter algicola gen. nov., sp.
31697228	12	55	theme	novel	1574:1578	arg1	genus					1580:1584	a novel genus	1572:1584	a novel genus	1572:1584	Based on the genotypic, chemotaxonomic and phenotypic results, strain Seoho-28T represents a novel genus and species, Paraconexibacter algicola gen. nov., sp.
31697228	8	56	theme	average	1004:1010	arg1	identity					1023:1030	average nucleotide identity	1004:1030	average nucleotide identity	1004:1030	Genomic comparisons of strain Seoho-28T with families in the order Solirubrobacterales were made using the Genome-to-Genome Distance Calculator, average nucleotide identity and average amino acid identity analyses (values indicated ≤14.9, ≤73.5 and ≤57.8 %, respectively).
31697228	10	57	theme	major	1361:1365	arg1	diphosphatidylglycerol					1280:1301	diphosphatidylglycerol	1280:1301	diphosphatidylglycerol	1280:1301	Strain Seoho-28T contained diphosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid as major polar lipids.
31697228	10	57	theme	major	1361:1365	arg1	phosphatidylinositol					1304:1323	phosphatidylinositol	1304:1323	phosphatidylinositol	1304:1323	Strain Seoho-28T contained diphosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid as major polar lipids.
31697228	10	57	theme	major	1361:1365	arg1	phospholipid					1345:1356	an unidentified phospholipid	1329:1356	an unidentified phospholipid	1329:1356	Strain Seoho-28T contained diphosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid as major polar lipids.
31697228	10	57	theme	major	1361:1365	arg1	lipids					1373:1378	major polar lipids	1361:1378	major polar lipids	1361:1378	Strain Seoho-28T contained diphosphatidylglycerol, phosphatidylinositol and an unidentified phospholipid as major polar lipids.
31697228	2	58	dep	nov.	200:203	arg1	Solirubrobacterales					218:236	the order Solirubrobacterales	208:236	the order Solirubrobacterales	208:236	nov. in the order Solirubrobacterales.
31697228	13	59	theme	family	1669:1674	arg1	Paraconexibacteraceae					1676:1696	a new family Paraconexibacteraceae	1663:1696	a new family Paraconexibacteraceae in the order Solirubrobacterales and the class Thermoleophilia	1663:1759	nov., which belongs to a new family Paraconexibacteraceae in the order Solirubrobacterales and the class Thermoleophilia.
31697228	2	60	theme	order	212:216	arg1	Solirubrobacterales					218:236	the order Solirubrobacterales	208:236	the order Solirubrobacterales	208:236	nov. in the order Solirubrobacterales.
31697228	7	61	theme	DNA	804:806	arg1	%					856:856	74.2 mol%	848:856	74.2 mol%	848:856	The genomic DNA G+C content of the strain Seoho-28T was 74.2 mol%.
31697228	7	61	theme	DNA	804:806	arg1	content					812:818	The genomic DNA G+C content	792:818	The genomic DNA G+C content of the strain Seoho-28T	792:842	The genomic DNA G+C content of the strain Seoho-28T was 74.2 mol%.
31697228	1	62	theme	fam	195:197	arg1	proposal					161:168	proposal	161:168	proposal of Paraconexibacteraceae fam	161:197	nov., a novel actinobacterium isolated from a eutrophic lake during the end of cyanobacterial harmful algal blooms, and proposal of Paraconexibacteraceae fam.
31697228	1	62	theme	fam	195:197	arg1	actinobacterium					55:69	a novel actinobacterium	47:69	a novel actinobacterium isolated from a eutrophic lake during the end of cyanobacterial harmful algal blooms	47:154	nov., a novel actinobacterium isolated from a eutrophic lake during the end of cyanobacterial harmful algal blooms, and proposal of Paraconexibacteraceae fam.
31697228	1	62	theme	fam	195:197	arg1	nov.					41:44	nov.	41:44	nov.	41:44	nov., a novel actinobacterium isolated from a eutrophic lake during the end of cyanobacterial harmful algal blooms, and proposal of Paraconexibacteraceae fam.
31697228	12	63	theme	strain	1544:1549	arg1	Seoho-28T					1551:1559	strain Seoho-28T	1544:1559	strain Seoho-28T	1544:1559	Based on the genotypic, chemotaxonomic and phenotypic results, strain Seoho-28T represents a novel genus and species, Paraconexibacter algicola gen. nov., sp.
31697228	4	64	theme	Gram-stain-negative	454:472	arg1	bacterium					510:518	a Gram-stain-negative, aerobic, rod-shaped and non-motile bacterium	452:518	a Gram-stain-negative, aerobic, rod-shaped and non-motile bacterium	452:518	Strain Seoho-28T was a Gram-stain-negative, aerobic, rod-shaped and non-motile bacterium.
31697228	4	64	theme	Gram-stain-negative	454:472	arg1	Seoho-28T					438:446	Strain Seoho-28T	431:446	Strain Seoho-28T	431:446	Strain Seoho-28T was a Gram-stain-negative, aerobic, rod-shaped and non-motile bacterium.
31697228	1	65	attach	isolated	71:78	arg1	lake					97:100	a eutrophic lake	85:100	a eutrophic lake during the end of cyanobacterial harmful algal blooms	85:154	nov., a novel actinobacterium isolated from a eutrophic lake during the end of cyanobacterial harmful algal blooms, and proposal of Paraconexibacteraceae fam.
31697228	1	65	attach	isolated	71:78	arg2	actinobacterium					55:69	a novel actinobacterium	47:69	a novel actinobacterium isolated from a eutrophic lake during the end of cyanobacterial harmful algal blooms	47:154	nov., a novel actinobacterium isolated from a eutrophic lake during the end of cyanobacterial harmful algal blooms, and proposal of Paraconexibacteraceae fam.
31697228	5	66	theme	0 	552:553	arg1	%					554:554	%	554:554	%	554:554	The strain grew optimally with 0 % NaCl and at 25-30 °C on Reasoner's 2A medium.
31697228	6	67	theme	rRNA	641:644	arg1	sequences					651:659	16S rRNA gene sequences	637:659	16S rRNA gene sequences	637:659	The phylogenetic analysis based on 16S rRNA gene sequences positioned the novel strain among the order Solirubrobacterales, but sequence similarities to known species were less than 94.7 %.
31697228	9	68	theme	major	1199:1203	arg1	C16 					1159:1162	C16 	1159:1162	C16 	1159:1162	Strain Seoho-28T contained C16 : 0-iso, C18 : 1 ω9c and C16 : 0 as major fatty acids and MK-7 (H4) as the major quinone.
31697228	9	68	theme	major	1199:1203	arg1	C16 					1188:1191	C16 	1188:1191	C16 	1188:1191	Strain Seoho-28T contained C16 : 0-iso, C18 : 1 ω9c and C16 : 0 as major fatty acids and MK-7 (H4) as the major quinone.
31697228	9	68	theme	major	1199:1203	arg1	acids					1211:1215	major fatty acids	1199:1215	major fatty acids	1199:1215	Strain Seoho-28T contained C16 : 0-iso, C18 : 1 ω9c and C16 : 0 as major fatty acids and MK-7 (H4) as the major quinone.
31697228	4	69	theme	Strain	431:436	arg1	bacterium					510:518	a Gram-stain-negative, aerobic, rod-shaped and non-motile bacterium	452:518	a Gram-stain-negative, aerobic, rod-shaped and non-motile bacterium	452:518	Strain Seoho-28T was a Gram-stain-negative, aerobic, rod-shaped and non-motile bacterium.
31697228	4	69	theme	Strain	431:436	arg1	Seoho-28T					438:446	Strain Seoho-28T	431:446	Strain Seoho-28T	431:446	Strain Seoho-28T was a Gram-stain-negative, aerobic, rod-shaped and non-motile bacterium.
31697228	11	70	theme	diamino	1435:1441	arg1	acids					1443:1447	the diagnostic diamino acids	1420:1447	the diagnostic diamino acids in the cell-wall peptidoglycan	1420:1478	Meso- and ll-diaminopimelic acids were the diagnostic diamino acids in the cell-wall peptidoglycan.
31697228	11	70	theme	diamino	1435:1441	arg1	acids					1409:1413	ll-diaminopimelic acids	1391:1413	ll-diaminopimelic acids	1391:1413	Meso- and ll-diaminopimelic acids were the diagnostic diamino acids in the cell-wall peptidoglycan.
31697228	11	70	theme	diamino	1435:1441	arg1	Meso-					1381:1385	Meso-	1381:1385	Meso-	1381:1385	Meso- and ll-diaminopimelic acids were the diagnostic diamino acids in the cell-wall peptidoglycan.
31697228	4	71	theme	non-motile	499:508	arg1	bacterium					510:518	a Gram-stain-negative, aerobic, rod-shaped and non-motile bacterium	452:518	a Gram-stain-negative, aerobic, rod-shaped and non-motile bacterium	452:518	Strain Seoho-28T was a Gram-stain-negative, aerobic, rod-shaped and non-motile bacterium.
31697228	4	71	theme	non-motile	499:508	arg1	Seoho-28T					438:446	Strain Seoho-28T	431:446	Strain Seoho-28T	431:446	Strain Seoho-28T was a Gram-stain-negative, aerobic, rod-shaped and non-motile bacterium.
31697228	8	72	theme	identity	1023:1030	arg1	Calculator					992:1001	the Genome-to-Genome Distance Calculator	962:1001	the Genome-to-Genome Distance Calculator	962:1001	Genomic comparisons of strain Seoho-28T with families in the order Solirubrobacterales were made using the Genome-to-Genome Distance Calculator, average nucleotide identity and average amino acid identity analyses (values indicated ≤14.9, ≤73.5 and ≤57.8 %, respectively).
31697228	8	72	theme	identity	1023:1030	arg1	analyses					1064:1071	average nucleotide identity and average amino acid identity analyses	1004:1071	average nucleotide identity and average amino acid identity analyses (values indicated ≤14.9, ≤73.5 and ≤57.8 %, respectively)	1004:1129	Genomic comparisons of strain Seoho-28T with families in the order Solirubrobacterales were made using the Genome-to-Genome Distance Calculator, average nucleotide identity and average amino acid identity analyses (values indicated ≤14.9, ≤73.5 and ≤57.8 %, respectively).
31697228	1	73	theme	harmful	135:141	arg1	blooms					149:154	cyanobacterial harmful algal blooms	120:154	cyanobacterial harmful algal blooms	120:154	nov., a novel actinobacterium isolated from a eutrophic lake during the end of cyanobacterial harmful algal blooms, and proposal of Paraconexibacteraceae fam.
31697228	3	74	theme	shallow	296:302	arg1	lake					314:317	a shallow eutrophic lake	294:317	a shallow eutrophic lake	294:317	A novel bacterium, strain Seoho-28T, was isolated from a shallow eutrophic lake during the end of cyanobacterial harmful algal blooms and was characterized taxonomically and phylogenetically.
31697228	11	75	theme	cell-wall	1456:1464	arg1	peptidoglycan					1466:1478	the cell-wall peptidoglycan	1452:1478	the cell-wall peptidoglycan	1452:1478	Meso- and ll-diaminopimelic acids were the diagnostic diamino acids in the cell-wall peptidoglycan.
31697228	13	76	from	Paraconexibacteraceae	1676:1696	arg1	Solirubrobacterales					1711:1729	the order Solirubrobacterales	1701:1729	the order Solirubrobacterales	1701:1729	nov., which belongs to a new family Paraconexibacteraceae in the order Solirubrobacterales and the class Thermoleophilia.
31697228	13	76	from	Paraconexibacteraceae	1676:1696	arg1	Thermoleophilia					1745:1759	the class Thermoleophilia	1735:1759	the class Thermoleophilia	1735:1759	nov., which belongs to a new family Paraconexibacteraceae in the order Solirubrobacterales and the class Thermoleophilia.
31697228	8	77	theme	average	1036:1042	arg1	identity					1055:1062	average amino acid identity	1036:1062	average amino acid identity	1036:1062	Genomic comparisons of strain Seoho-28T with families in the order Solirubrobacterales were made using the Genome-to-Genome Distance Calculator, average nucleotide identity and average amino acid identity analyses (values indicated ≤14.9, ≤73.5 and ≤57.8 %, respectively).
31697228	4	78	theme	aerobic	475:481	arg1	bacterium					510:518	a Gram-stain-negative, aerobic, rod-shaped and non-motile bacterium	452:518	a Gram-stain-negative, aerobic, rod-shaped and non-motile bacterium	452:518	Strain Seoho-28T was a Gram-stain-negative, aerobic, rod-shaped and non-motile bacterium.
31697228	4	78	theme	aerobic	475:481	arg1	Seoho-28T					438:446	Strain Seoho-28T	431:446	Strain Seoho-28T	431:446	Strain Seoho-28T was a Gram-stain-negative, aerobic, rod-shaped and non-motile bacterium.
31697228	13	79	theme	new	1665:1667	arg1	Paraconexibacteraceae					1676:1696	a new family Paraconexibacteraceae	1663:1696	a new family Paraconexibacteraceae in the order Solirubrobacterales and the class Thermoleophilia	1663:1759	nov., which belongs to a new family Paraconexibacteraceae in the order Solirubrobacterales and the class Thermoleophilia.
31697228	8	80	theme	acid	1050:1053	arg1	identity					1055:1062	average amino acid identity	1036:1062	average amino acid identity	1036:1062	Genomic comparisons of strain Seoho-28T with families in the order Solirubrobacterales were made using the Genome-to-Genome Distance Calculator, average nucleotide identity and average amino acid identity analyses (values indicated ≤14.9, ≤73.5 and ≤57.8 %, respectively).
31697228	1	81	theme	novel	49:53	arg1	actinobacterium					55:69	a novel actinobacterium	47:69	a novel actinobacterium isolated from a eutrophic lake during the end of cyanobacterial harmful algal blooms	47:154	nov., a novel actinobacterium isolated from a eutrophic lake during the end of cyanobacterial harmful algal blooms, and proposal of Paraconexibacteraceae fam.
31697228	12	82	theme	gen.	1625:1628	arg1	nov.					1630:1633	Paraconexibacter algicola gen. nov.	1599:1633	Paraconexibacter algicola gen. nov.	1599:1633	Based on the genotypic, chemotaxonomic and phenotypic results, strain Seoho-28T represents a novel genus and species, Paraconexibacter algicola gen. nov., sp.
31697228	12	82	theme	gen.	1625:1628	arg1	genus					1580:1584	a novel genus	1572:1584	a novel genus	1572:1584	Based on the genotypic, chemotaxonomic and phenotypic results, strain Seoho-28T represents a novel genus and species, Paraconexibacter algicola gen. nov., sp.
31697228	12	82	theme	gen.	1625:1628	arg1	sp					1636:1637	sp	1636:1637	sp	1636:1637	Based on the genotypic, chemotaxonomic and phenotypic results, strain Seoho-28T represents a novel genus and species, Paraconexibacter algicola gen. nov., sp.
31697228	4	83	theme	rod-shaped	484:493	arg1	bacterium					510:518	a Gram-stain-negative, aerobic, rod-shaped and non-motile bacterium	452:518	a Gram-stain-negative, aerobic, rod-shaped and non-motile bacterium	452:518	Strain Seoho-28T was a Gram-stain-negative, aerobic, rod-shaped and non-motile bacterium.
31697228	4	83	theme	rod-shaped	484:493	arg1	Seoho-28T					438:446	Strain Seoho-28T	431:446	Strain Seoho-28T	431:446	Strain Seoho-28T was a Gram-stain-negative, aerobic, rod-shaped and non-motile bacterium.
31697228	0	84	theme	algicola	17:24	arg1	nov.					31:34	Paraconexibacter algicola gen. nov.	0:34	Paraconexibacter algicola gen. nov.	0:34	Paraconexibacter algicola gen. nov., sp.
31697228	9	85	theme	major	1238:1242	arg1	quinone					1244:1250	the major quinone	1234:1250	the major quinone	1234:1250	Strain Seoho-28T contained C16 : 0-iso, C18 : 1 ω9c and C16 : 0 as major fatty acids and MK-7 (H4) as the major quinone.
31697228	9	85	theme	major	1238:1242	arg1	C16 					1159:1162	C16 	1159:1162	C16 	1159:1162	Strain Seoho-28T contained C16 : 0-iso, C18 : 1 ω9c and C16 : 0 as major fatty acids and MK-7 (H4) as the major quinone.
31697228	9	85	theme	major	1238:1242	arg1	C16 					1188:1191	C16 	1188:1191	C16 	1188:1191	Strain Seoho-28T contained C16 : 0-iso, C18 : 1 ω9c and C16 : 0 as major fatty acids and MK-7 (H4) as the major quinone.
31697228	3	86	theme	blooms	366:371	arg1	end					330:332	the end	326:332	the end of cyanobacterial harmful algal blooms	326:371	A novel bacterium, strain Seoho-28T, was isolated from a shallow eutrophic lake during the end of cyanobacterial harmful algal blooms and was characterized taxonomically and phylogenetically.
31697228	8	87	theme	Genomic	859:865	arg1	comparisons					867:877	Genomic comparisons	859:877	Genomic comparisons of strain Seoho-28T with families in the order Solirubrobacterales	859:944	Genomic comparisons of strain Seoho-28T with families in the order Solirubrobacterales were made using the Genome-to-Genome Distance Calculator, average nucleotide identity and average amino acid identity analyses (values indicated ≤14.9, ≤73.5 and ≤57.8 %, respectively).
31697228	3	88	theme	harmful	352:358	arg1	blooms					366:371	cyanobacterial harmful algal blooms	337:371	cyanobacterial harmful algal blooms	337:371	A novel bacterium, strain Seoho-28T, was isolated from a shallow eutrophic lake during the end of cyanobacterial harmful algal blooms and was characterized taxonomically and phylogenetically.
31697228	14	89	theme	type	1766:1769	arg1	strain					1771:1776	The type strain	1762:1776	The type strain	1762:1776	The type strain is Seoho-28T (=KCTC 39791T=JCM 31881T).
31697228	14	89	theme	type	1766:1769	arg1	Seoho-28T					1781:1789	Seoho-28T	1781:1789	Seoho-28T (=KCTC 39791T=JCM 31881T)	1781:1815	The type strain is Seoho-28T (=KCTC 39791T=JCM 31881T).
31697228	8	90	theme	≤14.9	1091:1095	arg1	%					1114:1114	≤14.9, ≤73.5 and ≤57.8 %	1091:1114	%	1114:1114	Genomic comparisons of strain Seoho-28T with families in the order Solirubrobacterales were made using the Genome-to-Genome Distance Calculator, average nucleotide identity and average amino acid identity analyses (values indicated ≤14.9, ≤73.5 and ≤57.8 %, respectively).
31697228	12	91	theme	Paraconexibacter	1599:1614	arg1	nov.					1630:1633	Paraconexibacter algicola gen. nov.	1599:1633	Paraconexibacter algicola gen. nov.	1599:1633	Based on the genotypic, chemotaxonomic and phenotypic results, strain Seoho-28T represents a novel genus and species, Paraconexibacter algicola gen. nov., sp.
31697228	12	91	theme	Paraconexibacter	1599:1614	arg1	genus					1580:1584	a novel genus	1572:1584	a novel genus	1572:1584	Based on the genotypic, chemotaxonomic and phenotypic results, strain Seoho-28T represents a novel genus and species, Paraconexibacter algicola gen. nov., sp.
31697228	12	91	theme	Paraconexibacter	1599:1614	arg1	sp					1636:1637	sp	1636:1637	sp	1636:1637	Based on the genotypic, chemotaxonomic and phenotypic results, strain Seoho-28T represents a novel genus and species, Paraconexibacter algicola gen. nov., sp.
31697228	6	92	theme	known	755:759	arg1	species					761:767	known species	755:767	known species	755:767	The phylogenetic analysis based on 16S rRNA gene sequences positioned the novel strain among the order Solirubrobacterales, but sequence similarities to known species were less than 94.7 %.
31697228	0	93	dep	sp	37:38	arg1	nov.					31:34	Paraconexibacter algicola gen. nov.	0:34	Paraconexibacter algicola gen. nov.	0:34	Paraconexibacter algicola gen. nov., sp.
31697228	8	94	theme	Seoho-28T	889:897	arg1	comparisons					867:877	Genomic comparisons	859:877	Genomic comparisons of strain Seoho-28T with families in the order Solirubrobacterales	859:944	Genomic comparisons of strain Seoho-28T with families in the order Solirubrobacterales were made using the Genome-to-Genome Distance Calculator, average nucleotide identity and average amino acid identity analyses (values indicated ≤14.9, ≤73.5 and ≤57.8 %, respectively).
31697228	8	95	theme	≤57.8 	1108:1113	arg1	%					1114:1114	≤14.9, ≤73.5 and ≤57.8 %	1091:1114	%	1114:1114	Genomic comparisons of strain Seoho-28T with families in the order Solirubrobacterales were made using the Genome-to-Genome Distance Calculator, average nucleotide identity and average amino acid identity analyses (values indicated ≤14.9, ≤73.5 and ≤57.8 %, respectively).
33736461	6	0	theme	nHA/CG	1160:1165	arg1	scaffolds					1167:1175	the nHA/CG scaffolds	1156:1175	the nHA/CG scaffolds	1156:1175	The hPDLSCs isolated from periodontal ligaments of discarded teeth (postorthodontic purposes) were seeded onto the nHA/CG scaffolds.
33736461	3	1	theme	previous	563:570	arg1	study					572:576	our previous study	559:576	our previous study	559:576	In our previous study, the efficiency of scaffolds with stem cell complexes in osteogenesis was confirmed in vivo in immunocompromised mice.
33736461	5	2	from	defects	1024:1030	arg1	minipigs					1035:1042	minipigs	1035:1042	minipigs	1035:1042	This study evaluated the bone regeneration potential of the nHA/CG scaffolds with transplanted human periodontal ligament stem cells (hPDLSCs) in critical-sized jaw bone defects in minipigs.
33736461	9	3	theme	control	1474:1480	arg1	group					1482:1486	a negative control group	1463:1486	a negative control group	1463:1486	The defects were randomly assigned to one of three groups [scaffolds with seeded hPDLSCs (hPDLSCs/nHA/CG), only scaffold (nHA/CG), and a negative control group, ie, no cells and scaffolds implanted into defects] to investigate jaw bone regeneration.
33736461	1	4	used	used	195:198	arg2	cells					179:183	Dental-tissue-derived stem cells	152:183	Dental-tissue-derived stem cells	152:183	Dental-tissue-derived stem cells have been used for tissue engineering owing to their ease of isolation and efficacy in in vitro and in vivo proliferation and differentiation.
33736461	3	5	theme	scaffolds	597:605	arg1	efficiency					583:592	the efficiency	579:592	the efficiency of scaffolds with stem cell complexes in osteogenesis	579:646	In our previous study, the efficiency of scaffolds with stem cell complexes in osteogenesis was confirmed in vivo in immunocompromised mice.
33736461	1	6	theme	in	285:286	arg1	proliferation					293:305	proliferation	293:305	proliferation	293:305	Dental-tissue-derived stem cells have been used for tissue engineering owing to their ease of isolation and efficacy in in vitro and in vivo proliferation and differentiation.
33736461	12	7	theme	bone	2058:2061	arg1	marrow					2063:2068	the bone marrow	2054:2068	the bone marrow formed in the hPDLSCs/nHA/CG group	2054:2103	Immunohistochemistry staining showed that runt-related transcription factor 2 (Runx2) was highly expressed in the bone marrow formed in the hPDLSCs/nHA/CG group.
33736461	1	8	from	differentiation	311:325	arg1	ease					238:241	their ease	232:241	their ease of isolation and efficacy in in vitro and in vivo proliferation and differentiation	232:325	Dental-tissue-derived stem cells have been used for tissue engineering owing to their ease of isolation and efficacy in in vitro and in vivo proliferation and differentiation.
33736461	1	9	theme	tissue	204:209	arg1	engineering					211:221	tissue engineering	204:221	tissue engineering owing to their ease of isolation and efficacy in in vitro and in vivo proliferation and differentiation	204:325	Dental-tissue-derived stem cells have been used for tissue engineering owing to their ease of isolation and efficacy in in vitro and in vivo proliferation and differentiation.
33736461	8	10	theme	jaw	1282:1284	arg1	defects					1291:1297	Forty-eight critical-sized jaw bone defects	1255:1297	Forty-eight critical-sized jaw bone defects	1255:1297	Forty-eight critical-sized jaw bone defects were created in 12 minipigs.
33736461	4	11	theme	bone	721:724	arg1	efficiency					739:748	the bone regeneration efficiency	717:748	the bone regeneration efficiency of stem cell-seeded nHA/CG scaffolds using large animal jaw bone defect models	717:827	However, studies on the bone regeneration efficiency of stem cell-seeded nHA/CG scaffolds using large animal jaw bone defect models have not been conducted.
33736461	11	12	theme	large	1835:1839	arg1	bones					1841:1845	large bones	1835:1845	large bones with normal architectures and vascularization	1835:1891	The results showed that the hPDLSCs adhered well to the nHA/CG scaffold in vitro, and the cell-nHA/CG composites significantly increased new bone formation and generated large bones with normal architectures and vascularization in vivo compared to the nHA/CG and control groups.
33736461	1	13	from	proliferation	293:305	arg1	ease					238:241	their ease	232:241	their ease of isolation and efficacy in in vitro and in vivo proliferation and differentiation	232:325	Dental-tissue-derived stem cells have been used for tissue engineering owing to their ease of isolation and efficacy in in vitro and in vivo proliferation and differentiation.
33736461	5	14	theme	transplanted	936:947	arg1	hPDLSCs					988:994	hPDLSCs	988:994	hPDLSCs	988:994	This study evaluated the bone regeneration potential of the nHA/CG scaffolds with transplanted human periodontal ligament stem cells (hPDLSCs) in critical-sized jaw bone defects in minipigs.
33736461	5	14	theme	transplanted	936:947	arg1	cells					981:985	transplanted human periodontal ligament stem cells	936:985	transplanted human periodontal ligament stem cells (hPDLSCs)	936:995	This study evaluated the bone regeneration potential of the nHA/CG scaffolds with transplanted human periodontal ligament stem cells (hPDLSCs) in critical-sized jaw bone defects in minipigs.
33736461	3	15	theme	stem	612:615	arg1	complexes					622:630	stem cell complexes	612:630	stem cell complexes in osteogenesis	612:646	In our previous study, the efficiency of scaffolds with stem cell complexes in osteogenesis was confirmed in vivo in immunocompromised mice.
33736461	13	16	theme	nHA/CG	2182:2187	arg1	scaffolds					2189:2197	the nHA/CG scaffolds	2178:2197	the nHA/CG scaffolds transplanted with hPDLSCs to regenerate the bone in large jaw bone defects	2178:2272	This study provides strong evidence for future clinical applications of the nHA/CG scaffolds transplanted with hPDLSCs to regenerate the bone in large jaw bone defects.
33736461	7	17	theme	previous	1237:1244	arg1	studies					1246:1252	our previous studies	1233:1252	our previous studies	1233:1252	The scaffold was successfully synthesized according to our previous studies.
33736461	1	18	from	ease	238:241	arg1	differentiation					311:325	differentiation	311:325	differentiation	311:325	Dental-tissue-derived stem cells have been used for tissue engineering owing to their ease of isolation and efficacy in in vitro and in vivo proliferation and differentiation.
33736461	1	18	from	ease	238:241	arg1	proliferation					293:305	proliferation	293:305	proliferation	293:305	Dental-tissue-derived stem cells have been used for tissue engineering owing to their ease of isolation and efficacy in in vitro and in vivo proliferation and differentiation.
33736461	4	19	theme	nHA/CG	770:775	arg1	scaffolds					777:785	stem cell-seeded nHA/CG scaffolds	753:785	stem cell-seeded nHA/CG scaffolds using large animal jaw bone defect models	753:827	However, studies on the bone regeneration efficiency of stem cell-seeded nHA/CG scaffolds using large animal jaw bone defect models have not been conducted.
33736461	1	20	dep	in	285:286	arg1	vivo					288:291	vivo	288:291	vivo	288:291	Dental-tissue-derived stem cells have been used for tissue engineering owing to their ease of isolation and efficacy in in vitro and in vivo proliferation and differentiation.
33736461	13	21	theme	clinical	2153:2160	arg1	applications					2162:2173	future clinical applications	2146:2173	future clinical applications of the nHA/CG scaffolds transplanted with hPDLSCs to regenerate the bone in large jaw bone defects	2146:2272	This study provides strong evidence for future clinical applications of the nHA/CG scaffolds transplanted with hPDLSCs to regenerate the bone in large jaw bone defects.
33736461	3	22	theme	immunocompromised	673:689	arg1	mice					691:694	immunocompromised mice	673:694	immunocompromised mice	673:694	In our previous study, the efficiency of scaffolds with stem cell complexes in osteogenesis was confirmed in vivo in immunocompromised mice.
33736461	1	23	theme	stem	174:177	arg1	cells					179:183	Dental-tissue-derived stem cells	152:183	Dental-tissue-derived stem cells	152:183	Dental-tissue-derived stem cells have been used for tissue engineering owing to their ease of isolation and efficacy in in vitro and in vivo proliferation and differentiation.
33736461	3	24	from	complexes	622:630	arg1	osteogenesis					635:646	osteogenesis	635:646	osteogenesis	635:646	In our previous study, the efficiency of scaffolds with stem cell complexes in osteogenesis was confirmed in vivo in immunocompromised mice.
33736461	0	25	theme	3D	92:93	arg1	Scaffolds					102:110	Nanohydroxyapatite/Chitosan/Gelatin 3D Porous Scaffolds	56:110	Nanohydroxyapatite/Chitosan/Gelatin 3D Porous Scaffolds	56:110	Human Periodontal Ligament Stem Cells Transplanted with Nanohydroxyapatite/Chitosan/Gelatin 3D Porous Scaffolds Promote Jaw Bone Regeneration in Swine.
33736461	5	26	theme	bone	879:882	arg1	potential					897:905	the bone regeneration potential	875:905	the bone regeneration potential of the nHA/CG scaffolds with transplanted human periodontal ligament stem cells (hPDLSCs) in critical-sized jaw bone defects in minipigs	875:1042	This study evaluated the bone regeneration potential of the nHA/CG scaffolds with transplanted human periodontal ligament stem cells (hPDLSCs) in critical-sized jaw bone defects in minipigs.
33736461	9	27	theme	bone	1559:1562	arg1	regeneration					1564:1575	jaw bone regeneration	1555:1575	jaw bone regeneration	1555:1575	The defects were randomly assigned to one of three groups [scaffolds with seeded hPDLSCs (hPDLSCs/nHA/CG), only scaffold (nHA/CG), and a negative control group, ie, no cells and scaffolds implanted into defects] to investigate jaw bone regeneration.
33736461	6	28	theme	periodontal	1071:1081	arg1	ligaments					1083:1091	periodontal ligaments	1071:1091	periodontal ligaments of discarded teeth (postorthodontic purposes)	1071:1137	The hPDLSCs isolated from periodontal ligaments of discarded teeth (postorthodontic purposes) were seeded onto the nHA/CG scaffolds.
33736461	4	29	theme	stem	753:756	arg1	scaffolds					777:785	stem cell-seeded nHA/CG scaffolds	753:785	stem cell-seeded nHA/CG scaffolds using large animal jaw bone defect models	753:827	However, studies on the bone regeneration efficiency of stem cell-seeded nHA/CG scaffolds using large animal jaw bone defect models have not been conducted.
33736461	11	30	theme	bone	1806:1809	arg1	formation					1811:1819	new bone formation	1802:1819	new bone formation	1802:1819	The results showed that the hPDLSCs adhered well to the nHA/CG scaffold in vitro, and the cell-nHA/CG composites significantly increased new bone formation and generated large bones with normal architectures and vascularization in vivo compared to the nHA/CG and control groups.
33736461	13	31	theme	strong	2126:2131	arg1	evidence					2133:2140	strong evidence	2126:2140	strong evidence for future clinical applications of the nHA/CG scaffolds transplanted with hPDLSCs to regenerate the bone in large jaw bone defects	2126:2272	This study provides strong evidence for future clinical applications of the nHA/CG scaffolds transplanted with hPDLSCs to regenerate the bone in large jaw bone defects.
33736461	5	32	theme	stem	976:979	arg1	hPDLSCs					988:994	hPDLSCs	988:994	hPDLSCs	988:994	This study evaluated the bone regeneration potential of the nHA/CG scaffolds with transplanted human periodontal ligament stem cells (hPDLSCs) in critical-sized jaw bone defects in minipigs.
33736461	5	32	theme	stem	976:979	arg1	cells					981:985	transplanted human periodontal ligament stem cells	936:985	transplanted human periodontal ligament stem cells (hPDLSCs)	936:995	This study evaluated the bone regeneration potential of the nHA/CG scaffolds with transplanted human periodontal ligament stem cells (hPDLSCs) in critical-sized jaw bone defects in minipigs.
33736461	0	33	theme	Jaw	120:122	arg1	Regeneration					129:140	Jaw Bone Regeneration	120:140	Jaw Bone Regeneration	120:140	Human Periodontal Ligament Stem Cells Transplanted with Nanohydroxyapatite/Chitosan/Gelatin 3D Porous Scaffolds Promote Jaw Bone Regeneration in Swine.
33736461	9	34	theme	[scaffolds	1386:1395	arg1	groups					1379:1384	three groups	1373:1384	three groups [scaffolds with seeded hPDLSCs (hPDLSCs/nHA/CG), only scaffold (nHA/CG), and a negative control group, ie, no cells and scaffolds implanted into defects]	1373:1538	The defects were randomly assigned to one of three groups [scaffolds with seeded hPDLSCs (hPDLSCs/nHA/CG), only scaffold (nHA/CG), and a negative control group, ie, no cells and scaffolds implanted into defects] to investigate jaw bone regeneration.
33736461	9	35	theme	seeded	1402:1407	arg1	hPDLSCs/nHA/CG					1418:1431	hPDLSCs/nHA/CG	1418:1431	hPDLSCs/nHA/CG	1418:1431	The defects were randomly assigned to one of three groups [scaffolds with seeded hPDLSCs (hPDLSCs/nHA/CG), only scaffold (nHA/CG), and a negative control group, ie, no cells and scaffolds implanted into defects] to investigate jaw bone regeneration.
33736461	9	35	theme	seeded	1402:1407	arg1	hPDLSCs					1409:1415	seeded hPDLSCs	1402:1415	seeded hPDLSCs (hPDLSCs/nHA/CG)	1402:1432	The defects were randomly assigned to one of three groups [scaffolds with seeded hPDLSCs (hPDLSCs/nHA/CG), only scaffold (nHA/CG), and a negative control group, ie, no cells and scaffolds implanted into defects] to investigate jaw bone regeneration.
33736461	2	36	theme	structural	502:511	arg1	similarity					528:537	their structural and functional similarity	496:537	their structural and functional similarity to natural bone	496:553	Nanohydroxyapatite/chitosan/gelatin (nHA/CG) three-dimensional porous scaffolds are promising for bone tissue engineering, especially jaw bone regeneration, because of their structural and functional similarity to natural bone.
33736461	4	37	theme	animal	799:804	arg1	models					822:827	large animal jaw bone defect models	793:827	large animal jaw bone defect models	793:827	However, studies on the bone regeneration efficiency of stem cell-seeded nHA/CG scaffolds using large animal jaw bone defect models have not been conducted.
33736461	11	38	theme	control	1928:1934	arg1	groups					1936:1941	the nHA/CG and control groups	1913:1941	groups	1936:1941	The results showed that the hPDLSCs adhered well to the nHA/CG scaffold in vitro, and the cell-nHA/CG composites significantly increased new bone formation and generated large bones with normal architectures and vascularization in vivo compared to the nHA/CG and control groups.
33736461	0	39	theme	Human	0:4	arg1	Cells					32:36	Human Periodontal Ligament Stem Cells	0:36	Human Periodontal Ligament Stem Cells Transplanted with Nanohydroxyapatite/Chitosan/Gelatin 3D Porous Scaffolds	0:110	Human Periodontal Ligament Stem Cells Transplanted with Nanohydroxyapatite/Chitosan/Gelatin 3D Porous Scaffolds Promote Jaw Bone Regeneration in Swine.
33736461	5	40	theme	critical-sized	1000:1013	arg1	defects					1024:1030	critical-sized jaw bone defects	1000:1030	critical-sized jaw bone defects in minipigs	1000:1042	This study evaluated the bone regeneration potential of the nHA/CG scaffolds with transplanted human periodontal ligament stem cells (hPDLSCs) in critical-sized jaw bone defects in minipigs.
33736461	4	41	theme	bone	810:813	arg1	models					822:827	large animal jaw bone defect models	793:827	large animal jaw bone defect models	793:827	However, studies on the bone regeneration efficiency of stem cell-seeded nHA/CG scaffolds using large animal jaw bone defect models have not been conducted.
33736461	0	42	theme	Ligament	18:25	arg1	Cells					32:36	Human Periodontal Ligament Stem Cells	0:36	Human Periodontal Ligament Stem Cells Transplanted with Nanohydroxyapatite/Chitosan/Gelatin 3D Porous Scaffolds	0:110	Human Periodontal Ligament Stem Cells Transplanted with Nanohydroxyapatite/Chitosan/Gelatin 3D Porous Scaffolds Promote Jaw Bone Regeneration in Swine.
33736461	5	43	theme	bone	1019:1022	arg1	defects					1024:1030	critical-sized jaw bone defects	1000:1030	critical-sized jaw bone defects in minipigs	1000:1042	This study evaluated the bone regeneration potential of the nHA/CG scaffolds with transplanted human periodontal ligament stem cells (hPDLSCs) in critical-sized jaw bone defects in minipigs.
33736461	6	44	theme	postorthodontic	1113:1127	arg1	teeth					1106:1110	discarded teeth	1096:1110	discarded teeth (postorthodontic purposes)	1096:1137	The hPDLSCs isolated from periodontal ligaments of discarded teeth (postorthodontic purposes) were seeded onto the nHA/CG scaffolds.
33736461	6	44	theme	postorthodontic	1113:1127	arg1	purposes					1129:1136	postorthodontic purposes	1113:1136	postorthodontic purposes	1113:1136	The hPDLSCs isolated from periodontal ligaments of discarded teeth (postorthodontic purposes) were seeded onto the nHA/CG scaffolds.
33736461	2	45	theme	bone	466:469	arg1	regeneration					471:482	jaw bone regeneration	462:482	especially jaw bone regeneration	451:482	Nanohydroxyapatite/chitosan/gelatin (nHA/CG) three-dimensional porous scaffolds are promising for bone tissue engineering, especially jaw bone regeneration, because of their structural and functional similarity to natural bone.
33736461	2	45	theme	bone	466:469	arg1	engineering					438:448	bone tissue engineering	426:448	bone tissue engineering	426:448	Nanohydroxyapatite/chitosan/gelatin (nHA/CG) three-dimensional porous scaffolds are promising for bone tissue engineering, especially jaw bone regeneration, because of their structural and functional similarity to natural bone.
33736461	9	46	theme	only	1435:1438	arg1	scaffold					1440:1447	only scaffold	1435:1447	only scaffold (nHA/CG)	1435:1456	The defects were randomly assigned to one of three groups [scaffolds with seeded hPDLSCs (hPDLSCs/nHA/CG), only scaffold (nHA/CG), and a negative control group, ie, no cells and scaffolds implanted into defects] to investigate jaw bone regeneration.
33736461	9	46	theme	only	1435:1438	arg1	nHA/CG					1450:1455	nHA/CG	1450:1455	nHA/CG	1450:1455	The defects were randomly assigned to one of three groups [scaffolds with seeded hPDLSCs (hPDLSCs/nHA/CG), only scaffold (nHA/CG), and a negative control group, ie, no cells and scaffolds implanted into defects] to investigate jaw bone regeneration.
33736461	6	47	theme	teeth	1106:1110	arg1	ligaments					1083:1091	periodontal ligaments	1071:1091	periodontal ligaments of discarded teeth (postorthodontic purposes)	1071:1137	The hPDLSCs isolated from periodontal ligaments of discarded teeth (postorthodontic purposes) were seeded onto the nHA/CG scaffolds.
33736461	2	48	theme	functional	517:526	arg1	similarity					528:537	their structural and functional similarity	496:537	their structural and functional similarity to natural bone	496:553	Nanohydroxyapatite/chitosan/gelatin (nHA/CG) three-dimensional porous scaffolds are promising for bone tissue engineering, especially jaw bone regeneration, because of their structural and functional similarity to natural bone.
33736461	13	49	theme	large	2251:2255	arg1	defects					2266:2272	large jaw bone defects	2251:2272	large jaw bone defects	2251:2272	This study provides strong evidence for future clinical applications of the nHA/CG scaffolds transplanted with hPDLSCs to regenerate the bone in large jaw bone defects.
33736461	1	50	link	Dental-tissue-derived	152:172	arg1	cells					179:183	Dental-tissue-derived stem cells	152:183	Dental-tissue-derived stem cells	152:183	Dental-tissue-derived stem cells have been used for tissue engineering owing to their ease of isolation and efficacy in in vitro and in vivo proliferation and differentiation.
33736461	3	51	with	scaffolds	597:605	arg1	complexes					622:630	stem cell complexes	612:630	stem cell complexes in osteogenesis	612:646	In our previous study, the efficiency of scaffolds with stem cell complexes in osteogenesis was confirmed in vivo in immunocompromised mice.
33736461	13	52	theme	bone	2261:2264	arg1	defects					2266:2272	large jaw bone defects	2251:2272	large jaw bone defects	2251:2272	This study provides strong evidence for future clinical applications of the nHA/CG scaffolds transplanted with hPDLSCs to regenerate the bone in large jaw bone defects.
33736461	2	53	theme	three-dimensional	373:389	arg1	scaffolds					398:406	Nanohydroxyapatite/chitosan/gelatin (nHA/CG) three-dimensional porous scaffolds	328:406	Nanohydroxyapatite/chitosan/gelatin (nHA/CG) three-dimensional porous scaffolds	328:406	Nanohydroxyapatite/chitosan/gelatin (nHA/CG) three-dimensional porous scaffolds are promising for bone tissue engineering, especially jaw bone regeneration, because of their structural and functional similarity to natural bone.
33736461	1	54	theme	isolation	246:254	arg1	ease					238:241	their ease	232:241	their ease of isolation and efficacy in in vitro and in vivo proliferation and differentiation	232:325	Dental-tissue-derived stem cells have been used for tissue engineering owing to their ease of isolation and efficacy in in vitro and in vivo proliferation and differentiation.
33736461	5	55	theme	periodontal	955:965	arg1	hPDLSCs					988:994	hPDLSCs	988:994	hPDLSCs	988:994	This study evaluated the bone regeneration potential of the nHA/CG scaffolds with transplanted human periodontal ligament stem cells (hPDLSCs) in critical-sized jaw bone defects in minipigs.
33736461	5	55	theme	periodontal	955:965	arg1	cells					981:985	transplanted human periodontal ligament stem cells	936:985	transplanted human periodontal ligament stem cells (hPDLSCs)	936:995	This study evaluated the bone regeneration potential of the nHA/CG scaffolds with transplanted human periodontal ligament stem cells (hPDLSCs) in critical-sized jaw bone defects in minipigs.
33736461	2	56	theme	bone	426:429	arg1	regeneration					471:482	jaw bone regeneration	462:482	especially jaw bone regeneration	451:482	Nanohydroxyapatite/chitosan/gelatin (nHA/CG) three-dimensional porous scaffolds are promising for bone tissue engineering, especially jaw bone regeneration, because of their structural and functional similarity to natural bone.
33736461	2	56	theme	bone	426:429	arg1	engineering					438:448	bone tissue engineering	426:448	bone tissue engineering	426:448	Nanohydroxyapatite/chitosan/gelatin (nHA/CG) three-dimensional porous scaffolds are promising for bone tissue engineering, especially jaw bone regeneration, because of their structural and functional similarity to natural bone.
33736461	5	57	theme	scaffolds	921:929	arg1	potential					897:905	the bone regeneration potential	875:905	the bone regeneration potential of the nHA/CG scaffolds with transplanted human periodontal ligament stem cells (hPDLSCs) in critical-sized jaw bone defects in minipigs	875:1042	This study evaluated the bone regeneration potential of the nHA/CG scaffolds with transplanted human periodontal ligament stem cells (hPDLSCs) in critical-sized jaw bone defects in minipigs.
33736461	1	58	theme	efficacy	260:267	arg1	ease					238:241	their ease	232:241	their ease of isolation and efficacy in in vitro and in vivo proliferation and differentiation	232:325	Dental-tissue-derived stem cells have been used for tissue engineering owing to their ease of isolation and efficacy in in vitro and in vivo proliferation and differentiation.
33736461	10	59	theme	bone	1582:1585	arg1	capacities					1600:1609	The bone regeneration capacities	1578:1609	The bone regeneration capacities of the three groups	1578:1629	The bone regeneration capacities of the three groups were assessed for up to 12 weeks.
33736461	10	60	dep	12	1655:1656	arg1	to					1652:1653	to	1652:1653	to	1652:1653	The bone regeneration capacities of the three groups were assessed for up to 12 weeks.
33736461	13	61	from	bone	2243:2246	arg1	defects					2266:2272	large jaw bone defects	2251:2272	large jaw bone defects	2251:2272	This study provides strong evidence for future clinical applications of the nHA/CG scaffolds transplanted with hPDLSCs to regenerate the bone in large jaw bone defects.
33736461	1	62	theme	in	272:273	arg1	proliferation					293:305	proliferation	293:305	proliferation	293:305	Dental-tissue-derived stem cells have been used for tissue engineering owing to their ease of isolation and efficacy in in vitro and in vivo proliferation and differentiation.
33736461	10	63	theme	groups	1624:1629	arg1	capacities					1600:1609	The bone regeneration capacities	1578:1609	The bone regeneration capacities of the three groups	1578:1629	The bone regeneration capacities of the three groups were assessed for up to 12 weeks.
33736461	6	64	attach	isolated	1057:1064	arg1	ligaments					1083:1091	periodontal ligaments	1071:1091	periodontal ligaments of discarded teeth (postorthodontic purposes)	1071:1137	The hPDLSCs isolated from periodontal ligaments of discarded teeth (postorthodontic purposes) were seeded onto the nHA/CG scaffolds.
33736461	6	64	attach	isolated	1057:1064	arg2	hPDLSCs					1049:1055	The hPDLSCs	1045:1055	The hPDLSCs isolated from periodontal ligaments of discarded teeth (postorthodontic purposes)	1045:1137	The hPDLSCs isolated from periodontal ligaments of discarded teeth (postorthodontic purposes) were seeded onto the nHA/CG scaffolds.
33736461	5	65	theme	nHA/CG	914:919	arg1	scaffolds					921:929	the nHA/CG scaffolds	910:929	the nHA/CG scaffolds with transplanted human periodontal ligament stem cells (hPDLSCs)	910:995	This study evaluated the bone regeneration potential of the nHA/CG scaffolds with transplanted human periodontal ligament stem cells (hPDLSCs) in critical-sized jaw bone defects in minipigs.
33736461	3	66	theme	cell	617:620	arg1	complexes					622:630	stem cell complexes	612:630	stem cell complexes in osteogenesis	612:646	In our previous study, the efficiency of scaffolds with stem cell complexes in osteogenesis was confirmed in vivo in immunocompromised mice.
33736461	4	67	from	studies	706:712	arg1	efficiency					739:748	the bone regeneration efficiency	717:748	the bone regeneration efficiency of stem cell-seeded nHA/CG scaffolds using large animal jaw bone defect models	717:827	However, studies on the bone regeneration efficiency of stem cell-seeded nHA/CG scaffolds using large animal jaw bone defect models have not been conducted.
33736461	9	68	theme	no	1493:1494	arg1	cells					1496:1500	no cells	1493:1500	no cells	1493:1500	The defects were randomly assigned to one of three groups [scaffolds with seeded hPDLSCs (hPDLSCs/nHA/CG), only scaffold (nHA/CG), and a negative control group, ie, no cells and scaffolds implanted into defects] to investigate jaw bone regeneration.
33736461	8	69	theme	critical-sized	1267:1280	arg1	defects					1291:1297	Forty-eight critical-sized jaw bone defects	1255:1297	Forty-eight critical-sized jaw bone defects	1255:1297	Forty-eight critical-sized jaw bone defects were created in 12 minipigs.
33736461	5	70	theme	regeneration	884:895	arg1	potential					897:905	the bone regeneration potential	875:905	the bone regeneration potential of the nHA/CG scaffolds with transplanted human periodontal ligament stem cells (hPDLSCs) in critical-sized jaw bone defects in minipigs	875:1042	This study evaluated the bone regeneration potential of the nHA/CG scaffolds with transplanted human periodontal ligament stem cells (hPDLSCs) in critical-sized jaw bone defects in minipigs.
33736461	8	71	theme	bone	1286:1289	arg1	defects					1291:1297	Forty-eight critical-sized jaw bone defects	1255:1297	Forty-eight critical-sized jaw bone defects	1255:1297	Forty-eight critical-sized jaw bone defects were created in 12 minipigs.
33736461	12	72	theme	transcription	1999:2011	arg1	factor					2013:2018	runt-related transcription factor 2	1986:2020	runt-related transcription factor 2 (Runx2)	1986:2028	Immunohistochemistry staining showed that runt-related transcription factor 2 (Runx2) was highly expressed in the bone marrow formed in the hPDLSCs/nHA/CG group.
33736461	12	72	theme	transcription	1999:2011	arg1	Runx2					2023:2027	Runx2	2023:2027	Runx2	2023:2027	Immunohistochemistry staining showed that runt-related transcription factor 2 (Runx2) was highly expressed in the bone marrow formed in the hPDLSCs/nHA/CG group.
33736461	1	73	from	efficacy	260:267	arg1	differentiation					311:325	differentiation	311:325	differentiation	311:325	Dental-tissue-derived stem cells have been used for tissue engineering owing to their ease of isolation and efficacy in in vitro and in vivo proliferation and differentiation.
33736461	1	73	from	efficacy	260:267	arg1	proliferation					293:305	proliferation	293:305	proliferation	293:305	Dental-tissue-derived stem cells have been used for tissue engineering owing to their ease of isolation and efficacy in in vitro and in vivo proliferation and differentiation.
33736461	13	74	theme	scaffolds	2189:2197	arg1	applications					2162:2173	future clinical applications	2146:2173	future clinical applications of the nHA/CG scaffolds transplanted with hPDLSCs to regenerate the bone in large jaw bone defects	2146:2272	This study provides strong evidence for future clinical applications of the nHA/CG scaffolds transplanted with hPDLSCs to regenerate the bone in large jaw bone defects.
33736461	4	75	theme	cell-seeded	758:768	arg1	scaffolds					777:785	stem cell-seeded nHA/CG scaffolds	753:785	stem cell-seeded nHA/CG scaffolds using large animal jaw bone defect models	753:827	However, studies on the bone regeneration efficiency of stem cell-seeded nHA/CG scaffolds using large animal jaw bone defect models have not been conducted.
33736461	11	76	theme	normal	1852:1857	arg1	architectures					1859:1871	normal architectures	1852:1871	normal architectures	1852:1871	The results showed that the hPDLSCs adhered well to the nHA/CG scaffold in vitro, and the cell-nHA/CG composites significantly increased new bone formation and generated large bones with normal architectures and vascularization in vivo compared to the nHA/CG and control groups.
33736461	1	77	from	isolation	246:254	arg1	differentiation					311:325	differentiation	311:325	differentiation	311:325	Dental-tissue-derived stem cells have been used for tissue engineering owing to their ease of isolation and efficacy in in vitro and in vivo proliferation and differentiation.
33736461	1	77	from	isolation	246:254	arg1	proliferation					293:305	proliferation	293:305	proliferation	293:305	Dental-tissue-derived stem cells have been used for tissue engineering owing to their ease of isolation and efficacy in in vitro and in vivo proliferation and differentiation.
33736461	13	78	theme	future	2146:2151	arg1	applications					2162:2173	future clinical applications	2146:2173	future clinical applications of the nHA/CG scaffolds transplanted with hPDLSCs to regenerate the bone in large jaw bone defects	2146:2272	This study provides strong evidence for future clinical applications of the nHA/CG scaffolds transplanted with hPDLSCs to regenerate the bone in large jaw bone defects.
33736461	5	79	from	potential	897:905	arg1	defects					1024:1030	critical-sized jaw bone defects	1000:1030	critical-sized jaw bone defects in minipigs	1000:1042	This study evaluated the bone regeneration potential of the nHA/CG scaffolds with transplanted human periodontal ligament stem cells (hPDLSCs) in critical-sized jaw bone defects in minipigs.
33736461	1	80	theme	Dental-tissue-derived	152:172	arg1	cells					179:183	Dental-tissue-derived stem cells	152:183	Dental-tissue-derived stem cells	152:183	Dental-tissue-derived stem cells have been used for tissue engineering owing to their ease of isolation and efficacy in in vitro and in vivo proliferation and differentiation.
33736461	6	81	theme	discarded	1096:1104	arg1	teeth					1106:1110	discarded teeth	1096:1110	discarded teeth (postorthodontic purposes)	1096:1137	The hPDLSCs isolated from periodontal ligaments of discarded teeth (postorthodontic purposes) were seeded onto the nHA/CG scaffolds.
33736461	6	81	theme	discarded	1096:1104	arg1	purposes					1129:1136	postorthodontic purposes	1113:1136	postorthodontic purposes	1113:1136	The hPDLSCs isolated from periodontal ligaments of discarded teeth (postorthodontic purposes) were seeded onto the nHA/CG scaffolds.
33736461	1	82	dep	in	272:273	arg1	vitro					275:279	vitro	275:279	vitro	275:279	Dental-tissue-derived stem cells have been used for tissue engineering owing to their ease of isolation and efficacy in in vitro and in vivo proliferation and differentiation.
33736461	4	83	theme	scaffolds	777:785	arg1	efficiency					739:748	the bone regeneration efficiency	717:748	the bone regeneration efficiency of stem cell-seeded nHA/CG scaffolds using large animal jaw bone defect models	717:827	However, studies on the bone regeneration efficiency of stem cell-seeded nHA/CG scaffolds using large animal jaw bone defect models have not been conducted.
33736461	9	84	with	[scaffolds	1386:1395	arg1	ie					1489:1490	ie	1489:1490	ie	1489:1490	The defects were randomly assigned to one of three groups [scaffolds with seeded hPDLSCs (hPDLSCs/nHA/CG), only scaffold (nHA/CG), and a negative control group, ie, no cells and scaffolds implanted into defects] to investigate jaw bone regeneration.
33736461	9	84	with	[scaffolds	1386:1395	arg1	hPDLSCs					1409:1415	seeded hPDLSCs	1402:1415	seeded hPDLSCs (hPDLSCs/nHA/CG)	1402:1432	The defects were randomly assigned to one of three groups [scaffolds with seeded hPDLSCs (hPDLSCs/nHA/CG), only scaffold (nHA/CG), and a negative control group, ie, no cells and scaffolds implanted into defects] to investigate jaw bone regeneration.
33736461	9	84	with	[scaffolds	1386:1395	arg1	scaffold					1440:1447	only scaffold	1435:1447	only scaffold (nHA/CG)	1435:1456	The defects were randomly assigned to one of three groups [scaffolds with seeded hPDLSCs (hPDLSCs/nHA/CG), only scaffold (nHA/CG), and a negative control group, ie, no cells and scaffolds implanted into defects] to investigate jaw bone regeneration.
33736461	9	84	with	[scaffolds	1386:1395	arg1	cells					1496:1500	no cells	1493:1500	no cells	1493:1500	The defects were randomly assigned to one of three groups [scaffolds with seeded hPDLSCs (hPDLSCs/nHA/CG), only scaffold (nHA/CG), and a negative control group, ie, no cells and scaffolds implanted into defects] to investigate jaw bone regeneration.
33736461	9	84	with	[scaffolds	1386:1395	arg1	nHA/CG					1450:1455	nHA/CG	1450:1455	nHA/CG	1450:1455	The defects were randomly assigned to one of three groups [scaffolds with seeded hPDLSCs (hPDLSCs/nHA/CG), only scaffold (nHA/CG), and a negative control group, ie, no cells and scaffolds implanted into defects] to investigate jaw bone regeneration.
33736461	9	84	with	[scaffolds	1386:1395	arg1	group					1482:1486	a negative control group	1463:1486	a negative control group	1463:1486	The defects were randomly assigned to one of three groups [scaffolds with seeded hPDLSCs (hPDLSCs/nHA/CG), only scaffold (nHA/CG), and a negative control group, ie, no cells and scaffolds implanted into defects] to investigate jaw bone regeneration.
33736461	9	84	with	[scaffolds	1386:1395	arg1	hPDLSCs/nHA/CG					1418:1431	hPDLSCs/nHA/CG	1418:1431	hPDLSCs/nHA/CG	1418:1431	The defects were randomly assigned to one of three groups [scaffolds with seeded hPDLSCs (hPDLSCs/nHA/CG), only scaffold (nHA/CG), and a negative control group, ie, no cells and scaffolds implanted into defects] to investigate jaw bone regeneration.
33736461	9	84	with	[scaffolds	1386:1395	arg1	scaffolds					1506:1514	scaffolds	1506:1514	scaffolds implanted into defects	1506:1537	The defects were randomly assigned to one of three groups [scaffolds with seeded hPDLSCs (hPDLSCs/nHA/CG), only scaffold (nHA/CG), and a negative control group, ie, no cells and scaffolds implanted into defects] to investigate jaw bone regeneration.
33736461	0	85	theme	Porous	95:100	arg1	Scaffolds					102:110	Nanohydroxyapatite/Chitosan/Gelatin 3D Porous Scaffolds	56:110	Nanohydroxyapatite/Chitosan/Gelatin 3D Porous Scaffolds	56:110	Human Periodontal Ligament Stem Cells Transplanted with Nanohydroxyapatite/Chitosan/Gelatin 3D Porous Scaffolds Promote Jaw Bone Regeneration in Swine.
33736461	9	86	theme	jaw	1555:1557	arg1	regeneration					1564:1575	jaw bone regeneration	1555:1575	jaw bone regeneration	1555:1575	The defects were randomly assigned to one of three groups [scaffolds with seeded hPDLSCs (hPDLSCs/nHA/CG), only scaffold (nHA/CG), and a negative control group, ie, no cells and scaffolds implanted into defects] to investigate jaw bone regeneration.
33736461	5	87	theme	human	949:953	arg1	hPDLSCs					988:994	hPDLSCs	988:994	hPDLSCs	988:994	This study evaluated the bone regeneration potential of the nHA/CG scaffolds with transplanted human periodontal ligament stem cells (hPDLSCs) in critical-sized jaw bone defects in minipigs.
33736461	5	87	theme	human	949:953	arg1	cells					981:985	transplanted human periodontal ligament stem cells	936:985	transplanted human periodontal ligament stem cells (hPDLSCs)	936:995	This study evaluated the bone regeneration potential of the nHA/CG scaffolds with transplanted human periodontal ligament stem cells (hPDLSCs) in critical-sized jaw bone defects in minipigs.
33736461	4	88	theme	regeneration	726:737	arg1	efficiency					739:748	the bone regeneration efficiency	717:748	the bone regeneration efficiency of stem cell-seeded nHA/CG scaffolds using large animal jaw bone defect models	717:827	However, studies on the bone regeneration efficiency of stem cell-seeded nHA/CG scaffolds using large animal jaw bone defect models have not been conducted.
33736461	11	89	theme	cell-nHA/CG	1755:1765	arg1	composites					1767:1776	the cell-nHA/CG composites	1751:1776	the cell-nHA/CG composites	1751:1776	The results showed that the hPDLSCs adhered well to the nHA/CG scaffold in vitro, and the cell-nHA/CG composites significantly increased new bone formation and generated large bones with normal architectures and vascularization in vivo compared to the nHA/CG and control groups.
33736461	5	90	theme	ligament	967:974	arg1	hPDLSCs					988:994	hPDLSCs	988:994	hPDLSCs	988:994	This study evaluated the bone regeneration potential of the nHA/CG scaffolds with transplanted human periodontal ligament stem cells (hPDLSCs) in critical-sized jaw bone defects in minipigs.
33736461	5	90	theme	ligament	967:974	arg1	cells					981:985	transplanted human periodontal ligament stem cells	936:985	transplanted human periodontal ligament stem cells (hPDLSCs)	936:995	This study evaluated the bone regeneration potential of the nHA/CG scaffolds with transplanted human periodontal ligament stem cells (hPDLSCs) in critical-sized jaw bone defects in minipigs.
33736461	2	91	theme	natural	542:548	arg1	bone					550:553	natural bone	542:553	natural bone	542:553	Nanohydroxyapatite/chitosan/gelatin (nHA/CG) three-dimensional porous scaffolds are promising for bone tissue engineering, especially jaw bone regeneration, because of their structural and functional similarity to natural bone.
33736461	0	92	theme	Bone	124:127	arg1	Regeneration					129:140	Jaw Bone Regeneration	120:140	Jaw Bone Regeneration	120:140	Human Periodontal Ligament Stem Cells Transplanted with Nanohydroxyapatite/Chitosan/Gelatin 3D Porous Scaffolds Promote Jaw Bone Regeneration in Swine.
33736461	9	93	theme	groups	1379:1384	arg1	one					1366:1368	one	1366:1368	one	1366:1368	The defects were randomly assigned to one of three groups [scaffolds with seeded hPDLSCs (hPDLSCs/nHA/CG), only scaffold (nHA/CG), and a negative control group, ie, no cells and scaffolds implanted into defects] to investigate jaw bone regeneration.
33736461	9	93	theme	groups	1379:1384	arg1	groups					1379:1384	three groups	1373:1384	three groups [scaffolds with seeded hPDLSCs (hPDLSCs/nHA/CG), only scaffold (nHA/CG), and a negative control group, ie, no cells and scaffolds implanted into defects]	1373:1538	The defects were randomly assigned to one of three groups [scaffolds with seeded hPDLSCs (hPDLSCs/nHA/CG), only scaffold (nHA/CG), and a negative control group, ie, no cells and scaffolds implanted into defects] to investigate jaw bone regeneration.
33736461	12	94	theme	runt-related	1986:1997	arg1	factor					2013:2018	runt-related transcription factor 2	1986:2020	runt-related transcription factor 2 (Runx2)	1986:2028	Immunohistochemistry staining showed that runt-related transcription factor 2 (Runx2) was highly expressed in the bone marrow formed in the hPDLSCs/nHA/CG group.
33736461	12	94	theme	runt-related	1986:1997	arg1	Runx2					2023:2027	Runx2	2023:2027	Runx2	2023:2027	Immunohistochemistry staining showed that runt-related transcription factor 2 (Runx2) was highly expressed in the bone marrow formed in the hPDLSCs/nHA/CG group.
33736461	4	95	theme	defect	815:820	arg1	models					822:827	large animal jaw bone defect models	793:827	large animal jaw bone defect models	793:827	However, studies on the bone regeneration efficiency of stem cell-seeded nHA/CG scaffolds using large animal jaw bone defect models have not been conducted.
33736461	11	96	theme	new	1802:1804	arg1	formation					1811:1819	new bone formation	1802:1819	new bone formation	1802:1819	The results showed that the hPDLSCs adhered well to the nHA/CG scaffold in vitro, and the cell-nHA/CG composites significantly increased new bone formation and generated large bones with normal architectures and vascularization in vivo compared to the nHA/CG and control groups.
33736461	0	97	theme	Periodontal	6:16	arg1	Cells					32:36	Human Periodontal Ligament Stem Cells	0:36	Human Periodontal Ligament Stem Cells Transplanted with Nanohydroxyapatite/Chitosan/Gelatin 3D Porous Scaffolds	0:110	Human Periodontal Ligament Stem Cells Transplanted with Nanohydroxyapatite/Chitosan/Gelatin 3D Porous Scaffolds Promote Jaw Bone Regeneration in Swine.
33736461	12	98	theme	Immunohistochemistry	1944:1963	arg1	staining					1965:1972	Immunohistochemistry staining	1944:1972	Immunohistochemistry staining	1944:1972	Immunohistochemistry staining showed that runt-related transcription factor 2 (Runx2) was highly expressed in the bone marrow formed in the hPDLSCs/nHA/CG group.
33736461	4	99	theme	large	793:797	arg1	models					822:827	large animal jaw bone defect models	793:827	large animal jaw bone defect models	793:827	However, studies on the bone regeneration efficiency of stem cell-seeded nHA/CG scaffolds using large animal jaw bone defect models have not been conducted.
33736461	2	100	theme	tissue	431:436	arg1	regeneration					471:482	jaw bone regeneration	462:482	especially jaw bone regeneration	451:482	Nanohydroxyapatite/chitosan/gelatin (nHA/CG) three-dimensional porous scaffolds are promising for bone tissue engineering, especially jaw bone regeneration, because of their structural and functional similarity to natural bone.
33736461	2	100	theme	tissue	431:436	arg1	engineering					438:448	bone tissue engineering	426:448	bone tissue engineering	426:448	Nanohydroxyapatite/chitosan/gelatin (nHA/CG) three-dimensional porous scaffolds are promising for bone tissue engineering, especially jaw bone regeneration, because of their structural and functional similarity to natural bone.
33736461	0	101	theme	Stem	27:30	arg1	Cells					32:36	Human Periodontal Ligament Stem Cells	0:36	Human Periodontal Ligament Stem Cells Transplanted with Nanohydroxyapatite/Chitosan/Gelatin 3D Porous Scaffolds	0:110	Human Periodontal Ligament Stem Cells Transplanted with Nanohydroxyapatite/Chitosan/Gelatin 3D Porous Scaffolds Promote Jaw Bone Regeneration in Swine.
33736461	11	102	with	bones	1841:1845	arg1	vascularization					1877:1891	vascularization	1877:1891	vascularization	1877:1891	The results showed that the hPDLSCs adhered well to the nHA/CG scaffold in vitro, and the cell-nHA/CG composites significantly increased new bone formation and generated large bones with normal architectures and vascularization in vivo compared to the nHA/CG and control groups.
33736461	11	102	with	bones	1841:1845	arg1	architectures					1859:1871	normal architectures	1852:1871	normal architectures	1852:1871	The results showed that the hPDLSCs adhered well to the nHA/CG scaffold in vitro, and the cell-nHA/CG composites significantly increased new bone formation and generated large bones with normal architectures and vascularization in vivo compared to the nHA/CG and control groups.
33736461	5	103	theme	jaw	1015:1017	arg1	defects					1024:1030	critical-sized jaw bone defects	1000:1030	critical-sized jaw bone defects in minipigs	1000:1042	This study evaluated the bone regeneration potential of the nHA/CG scaffolds with transplanted human periodontal ligament stem cells (hPDLSCs) in critical-sized jaw bone defects in minipigs.
33736461	11	104	theme	nHA/CG	1721:1726	arg1	scaffold					1728:1735	the nHA/CG scaffold	1717:1735	the nHA/CG scaffold	1717:1735	The results showed that the hPDLSCs adhered well to the nHA/CG scaffold in vitro, and the cell-nHA/CG composites significantly increased new bone formation and generated large bones with normal architectures and vascularization in vivo compared to the nHA/CG and control groups.
33736461	4	105	theme	jaw	806:808	arg1	models					822:827	large animal jaw bone defect models	793:827	large animal jaw bone defect models	793:827	However, studies on the bone regeneration efficiency of stem cell-seeded nHA/CG scaffolds using large animal jaw bone defect models have not been conducted.
33736461	11	106	theme	nHA/CG	1917:1922	arg1	groups					1936:1941	the nHA/CG and control groups	1913:1941	groups	1936:1941	The results showed that the hPDLSCs adhered well to the nHA/CG scaffold in vitro, and the cell-nHA/CG composites significantly increased new bone formation and generated large bones with normal architectures and vascularization in vivo compared to the nHA/CG and control groups.
33736461	12	107	theme	hPDLSCs/nHA/CG	2084:2097	arg1	group					2099:2103	the hPDLSCs/nHA/CG group	2080:2103	the hPDLSCs/nHA/CG group	2080:2103	Immunohistochemistry staining showed that runt-related transcription factor 2 (Runx2) was highly expressed in the bone marrow formed in the hPDLSCs/nHA/CG group.
33736461	9	108	theme	negative	1465:1472	arg1	group					1482:1486	a negative control group	1463:1486	a negative control group	1463:1486	The defects were randomly assigned to one of three groups [scaffolds with seeded hPDLSCs (hPDLSCs/nHA/CG), only scaffold (nHA/CG), and a negative control group, ie, no cells and scaffolds implanted into defects] to investigate jaw bone regeneration.
33736461	2	109	theme	jaw	462:464	arg1	regeneration					471:482	jaw bone regeneration	462:482	especially jaw bone regeneration	451:482	Nanohydroxyapatite/chitosan/gelatin (nHA/CG) three-dimensional porous scaffolds are promising for bone tissue engineering, especially jaw bone regeneration, because of their structural and functional similarity to natural bone.
33736461	2	109	theme	jaw	462:464	arg1	engineering					438:448	bone tissue engineering	426:448	bone tissue engineering	426:448	Nanohydroxyapatite/chitosan/gelatin (nHA/CG) three-dimensional porous scaffolds are promising for bone tissue engineering, especially jaw bone regeneration, because of their structural and functional similarity to natural bone.
33736461	0	110	theme	Nanohydroxyapatite/Chitosan/Gelatin	56:90	arg1	Scaffolds					102:110	Nanohydroxyapatite/Chitosan/Gelatin 3D Porous Scaffolds	56:110	Nanohydroxyapatite/Chitosan/Gelatin 3D Porous Scaffolds	56:110	Human Periodontal Ligament Stem Cells Transplanted with Nanohydroxyapatite/Chitosan/Gelatin 3D Porous Scaffolds Promote Jaw Bone Regeneration in Swine.
33736461	13	111	theme	jaw	2257:2259	arg1	defects					2266:2272	large jaw bone defects	2251:2272	large jaw bone defects	2251:2272	This study provides strong evidence for future clinical applications of the nHA/CG scaffolds transplanted with hPDLSCs to regenerate the bone in large jaw bone defects.
33736461	2	112	theme	porous	391:396	arg1	scaffolds					398:406	Nanohydroxyapatite/chitosan/gelatin (nHA/CG) three-dimensional porous scaffolds	328:406	Nanohydroxyapatite/chitosan/gelatin (nHA/CG) three-dimensional porous scaffolds	328:406	Nanohydroxyapatite/chitosan/gelatin (nHA/CG) three-dimensional porous scaffolds are promising for bone tissue engineering, especially jaw bone regeneration, because of their structural and functional similarity to natural bone.
33736461	5	113	with	scaffolds	921:929	arg1	hPDLSCs					988:994	hPDLSCs	988:994	hPDLSCs	988:994	This study evaluated the bone regeneration potential of the nHA/CG scaffolds with transplanted human periodontal ligament stem cells (hPDLSCs) in critical-sized jaw bone defects in minipigs.
33736461	5	113	with	scaffolds	921:929	arg1	cells					981:985	transplanted human periodontal ligament stem cells	936:985	transplanted human periodontal ligament stem cells (hPDLSCs)	936:995	This study evaluated the bone regeneration potential of the nHA/CG scaffolds with transplanted human periodontal ligament stem cells (hPDLSCs) in critical-sized jaw bone defects in minipigs.
33736461	10	114	theme	regeneration	1587:1598	arg1	capacities					1600:1609	The bone regeneration capacities	1578:1609	The bone regeneration capacities of the three groups	1578:1629	The bone regeneration capacities of the three groups were assessed for up to 12 weeks.
32941125	16	0	theme	49411T=CGMCC	2058:2069	arg1	52915T					2044:2049	the type strain SCSIO 52915T	2022:2049	the type strain SCSIO 52915T (=KCTC 49411T=CGMCC 1.13852T)	2022:2079	nov., with the type strain SCSIO 52915T (=KCTC 49411T=CGMCC 1.13852T), are proposed.
32941125	16	0	theme	49411T=CGMCC	2058:2069	arg1	1.13852T					2071:2078	=KCTC 49411T=CGMCC 1.13852T	2052:2078	=KCTC 49411T=CGMCC 1.13852T	2052:2078	nov., with the type strain SCSIO 52915T (=KCTC 49411T=CGMCC 1.13852T), are proposed.
32941125	16	1	theme	=KCTC	2052:2056	arg1	52915T					2044:2049	the type strain SCSIO 52915T	2022:2049	the type strain SCSIO 52915T (=KCTC 49411T=CGMCC 1.13852T)	2022:2079	nov., with the type strain SCSIO 52915T (=KCTC 49411T=CGMCC 1.13852T), are proposed.
32941125	16	1	theme	=KCTC	2052:2056	arg1	1.13852T					2071:2078	=KCTC 49411T=CGMCC 1.13852T	2052:2078	=KCTC 49411T=CGMCC 1.13852T	2052:2078	nov., with the type strain SCSIO 52915T (=KCTC 49411T=CGMCC 1.13852T), are proposed.
32941125	10	2	from	%	1076:1076	arg1	52915T					1087:1092	SCSIO 52915T	1081:1092	SCSIO 52915T	1081:1092	Genome sequencing revealed two large genomes of 4.58 Mbp with G+C content of 67.0 mol% in SCSIO 52909T and of 4.42 Mbp with G+C content of 69.1 % in SCSIO 52915T.
32941125	9	3	dep	components	899:908	arg1	12-methyl					921:929	 0 12-methyl	918:929	iso-branched components of C16 : 0 12-methyl	886:929	Their major fatty acids differed, but both contained iso-branched components of C16 : 0 12-methyl.
32941125	13	4	theme	known	1709:1713	arg1	species					1715:1721	the other known species	1699:1721	the other known species in the genus Rubrobacter	1699:1746	In the phylogenetic analysis, strains SCSIO 52909T and SCSIO 52915T were separately clustered together and formed a well-separated phylogenetic branch distinct from the other known species in the genus Rubrobacter.
32941125	1	5	theme	Rubrobacter	34:44	arg1	marinus					46:52	nov. and Rubrobacter marinus	25:52	nov. and Rubrobacter marinus	25:52	nov. and Rubrobacter marinus sp.
32941125	15	6	with	sp	2007:2008	arg1	52909T					1945:1950	the type strain SCSIO 52909T	1923:1950	the type strain SCSIO 52909T (=KCTC 49412T=CGMCC 1.13853T)	1923:1980	nov., with the type strain SCSIO 52909T (=KCTC 49412T=CGMCC 1.13853T), and Rubrobacter marinus sp.
32941125	15	6	with	sp	2007:2008	arg1	1.13853T					1972:1979	=KCTC 49412T=CGMCC 1.13853T	1953:1979	=KCTC 49412T=CGMCC 1.13853T	1953:1979	nov., with the type strain SCSIO 52909T (=KCTC 49412T=CGMCC 1.13853T), and Rubrobacter marinus sp.
32941125	5	7	theme	nitrate	478:484	arg1	reduction					486:494	nitrate reduction	478:494	nitrate reduction	478:494	These strains were aerobic and tested positive for catalase activity, oxidase activity and nitrate reduction.
32941125	12	8	theme	rRNA	1314:1317	arg1	Results					1290:1296	Results	1290:1296	Results of gANI and 16S rRNA gene analyses	1290:1331	Results of gANI and 16S rRNA gene analyses suggested that the two strains represent two new species, with 74.9, 95.0 % pairwise similarity between each other, and less than 74.3 and 93.5 % to other recognized Rubrobacter species, respectively.
32941125	6	9	theme	14 days	557:563	arg1	cultivation					565:575	14 days cultivation	557:575	14 days cultivation	557:575	Optimal growth occurred at 28 °C, pH 7 and 3% salinity over 14 days cultivation.
32941125	5	10	theme	catalase	438:445	arg1	activity					447:454	catalase activity	438:454	catalase activity	438:454	These strains were aerobic and tested positive for catalase activity, oxidase activity and nitrate reduction.
32941125	11	11	theme	thioredoxin	1267:1277	arg1	reductase					1279:1287	thioredoxin reductase	1267:1287	thioredoxin reductase	1267:1287	The two novel strains encoded genes for metabolism that are absent in most other Rubrobacter species, and possessed many more gene copy numbers of alkaline phosphatase and thioredoxin reductase.
32941125	10	12	theme	%	1017:1017	arg1	content					998:1004	G+C content	994:1004	G+C content of 67.0 mol% in SCSIO 52909T and of 4.42 Mbp	994:1049	Genome sequencing revealed two large genomes of 4.58 Mbp with G+C content of 67.0 mol% in SCSIO 52909T and of 4.42 Mbp with G+C content of 69.1 % in SCSIO 52915T.
32941125	3	13	theme	sediment	235:242	arg1	sample					244:249	a deep-sea sediment sample	224:249	a deep-sea sediment sample collected at about 3448 m water depth of the South China Sea	224:310	Two novel Gram-stain-positive bacteria, designated as SCSIO 52909T and SCSIO 52915T, were isolated from a deep-sea sediment sample collected at about 3448 m water depth of the South China Sea.
32941125	6	14	theme	3	540:540	arg1	%					541:541	%	541:541	%	541:541	Optimal growth occurred at 28 °C, pH 7 and 3% salinity over 14 days cultivation.
32941125	7	15	theme	only	641:644	arg1	menaquinone					646:656	the only menaquinone	637:656	the only menaquinone	637:656	Its peptidoglycan structure was type A3α (l-Lys-l-Ala) and the only menaquinone was MK-8.
32941125	7	15	theme	only	641:644	arg1	MK-8					662:665	MK-8	662:665	MK-8	662:665	Its peptidoglycan structure was type A3α (l-Lys-l-Ala) and the only menaquinone was MK-8.
32941125	13	16	theme	phylogenetic	1665:1676	arg1	branch					1678:1683	a well-separated phylogenetic branch	1648:1683	a well-separated phylogenetic branch distinct from the other known species in the genus Rubrobacter	1648:1746	In the phylogenetic analysis, strains SCSIO 52909T and SCSIO 52915T were separately clustered together and formed a well-separated phylogenetic branch distinct from the other known species in the genus Rubrobacter.
32941125	3	17	dep	depth	283:287	arg1	m					275:275	m	275:275	m	275:275	Two novel Gram-stain-positive bacteria, designated as SCSIO 52909T and SCSIO 52915T, were isolated from a deep-sea sediment sample collected at about 3448 m water depth of the South China Sea.
32941125	8	18	theme	unidentified	806:817	arg1	phospholipid					819:830	an unidentified phospholipid	803:830	an unidentified phospholipid	803:830	Both strains possessed diphosphatidylglycerol, phosphatidylglycerol, an unidentified phosphoglycolipid, an unidentified glycolipid and an unidentified phospholipid.
32941125	12	19	theme	recognized	1488:1497	arg1	species					1511:1517	other recognized Rubrobacter species	1482:1517	other recognized Rubrobacter species	1482:1517	Results of gANI and 16S rRNA gene analyses suggested that the two strains represent two new species, with 74.9, 95.0 % pairwise similarity between each other, and less than 74.3 and 93.5 % to other recognized Rubrobacter species, respectively.
32941125	1	20	dep	sp	54:55	arg1	marinus					46:52	nov. and Rubrobacter marinus	25:52	nov. and Rubrobacter marinus	25:52	nov. and Rubrobacter marinus sp.
32941125	8	21	theme	unidentified	740:751	arg1	phosphoglycolipid					753:769	an unidentified phosphoglycolipid	737:769	an unidentified phosphoglycolipid	737:769	Both strains possessed diphosphatidylglycerol, phosphatidylglycerol, an unidentified phosphoglycolipid, an unidentified glycolipid and an unidentified phospholipid.
32941125	10	22	theme	SCSIO	1022:1026	arg1	52909T					1028:1033	SCSIO 52909T	1022:1033	SCSIO 52909T	1022:1033	Genome sequencing revealed two large genomes of 4.58 Mbp with G+C content of 67.0 mol% in SCSIO 52909T and of 4.42 Mbp with G+C content of 69.1 % in SCSIO 52915T.
32941125	3	23	theme	novel	124:128	arg1	bacteria					150:157	Two novel Gram-stain-positive bacteria	120:157	Two novel Gram-stain-positive bacteria	120:157	Two novel Gram-stain-positive bacteria, designated as SCSIO 52909T and SCSIO 52915T, were isolated from a deep-sea sediment sample collected at about 3448 m water depth of the South China Sea.
32941125	11	24	from	absent	1155:1160	arg1	species					1188:1194	most other Rubrobacter species	1165:1194	most other Rubrobacter species	1165:1194	The two novel strains encoded genes for metabolism that are absent in most other Rubrobacter species, and possessed many more gene copy numbers of alkaline phosphatase and thioredoxin reductase.
32941125	10	25	from	content	998:1004	arg1	52909T					1028:1033	SCSIO 52909T	1022:1033	SCSIO 52909T	1022:1033	Genome sequencing revealed two large genomes of 4.58 Mbp with G+C content of 67.0 mol% in SCSIO 52909T and of 4.42 Mbp with G+C content of 69.1 % in SCSIO 52915T.
32941125	11	26	theme	gene	1221:1224	arg1	numbers					1231:1237	many more gene copy numbers	1211:1237	many more gene copy numbers of alkaline phosphatase and thioredoxin reductase	1211:1287	The two novel strains encoded genes for metabolism that are absent in most other Rubrobacter species, and possessed many more gene copy numbers of alkaline phosphatase and thioredoxin reductase.
32941125	8	27	theme	unidentified	775:786	arg1	glycolipid					788:797	an unidentified glycolipid	772:797	an unidentified glycolipid	772:797	Both strains possessed diphosphatidylglycerol, phosphatidylglycerol, an unidentified phosphoglycolipid, an unidentified glycolipid and an unidentified phospholipid.
32941125	15	28	theme	type	1927:1930	arg1	52909T					1945:1950	the type strain SCSIO 52909T	1923:1950	the type strain SCSIO 52909T (=KCTC 49412T=CGMCC 1.13853T)	1923:1980	nov., with the type strain SCSIO 52909T (=KCTC 49412T=CGMCC 1.13853T), and Rubrobacter marinus sp.
32941125	15	28	theme	type	1927:1930	arg1	1.13853T					1972:1979	=KCTC 49412T=CGMCC 1.13853T	1953:1979	=KCTC 49412T=CGMCC 1.13853T	1953:1979	nov., with the type strain SCSIO 52909T (=KCTC 49412T=CGMCC 1.13853T), and Rubrobacter marinus sp.
32941125	9	29	contain	contained	876:884	arg1	both					871:874	both	871:874	both	871:874	Their major fatty acids differed, but both contained iso-branched components of C16 : 0 12-methyl.
32941125	9	29	contain	contained	876:884	arg2	components					899:908	iso-branched components	886:908	iso-branched components of C16 : 0 12-methyl	886:929	Their major fatty acids differed, but both contained iso-branched components of C16 : 0 12-methyl.
32941125	11	30	theme	many	1211:1214	arg1	numbers					1231:1237	many more gene copy numbers	1211:1237	many more gene copy numbers of alkaline phosphatase and thioredoxin reductase	1211:1287	The two novel strains encoded genes for metabolism that are absent in most other Rubrobacter species, and possessed many more gene copy numbers of alkaline phosphatase and thioredoxin reductase.
32941125	7	31	theme	peptidoglycan	582:594	arg1	structure					596:604	Its peptidoglycan structure	578:604	Its peptidoglycan structure	578:604	Its peptidoglycan structure was type A3α (l-Lys-l-Ala) and the only menaquinone was MK-8.
32941125	15	32	theme	=KCTC	1953:1957	arg1	52909T					1945:1950	the type strain SCSIO 52909T	1923:1950	the type strain SCSIO 52909T (=KCTC 49412T=CGMCC 1.13853T)	1923:1980	nov., with the type strain SCSIO 52909T (=KCTC 49412T=CGMCC 1.13853T), and Rubrobacter marinus sp.
32941125	15	32	theme	=KCTC	1953:1957	arg1	1.13853T					1972:1979	=KCTC 49412T=CGMCC 1.13853T	1953:1979	=KCTC 49412T=CGMCC 1.13853T	1953:1979	nov., with the type strain SCSIO 52909T (=KCTC 49412T=CGMCC 1.13853T), and Rubrobacter marinus sp.
32941125	3	33	theme	China	302:306	arg1	Sea					308:310	the South China Sea	292:310	the South China Sea	292:310	Two novel Gram-stain-positive bacteria, designated as SCSIO 52909T and SCSIO 52915T, were isolated from a deep-sea sediment sample collected at about 3448 m water depth of the South China Sea.
32941125	11	34	theme	alkaline	1242:1249	arg1	phosphatase					1251:1261	alkaline phosphatase	1242:1261	alkaline phosphatase	1242:1261	The two novel strains encoded genes for metabolism that are absent in most other Rubrobacter species, and possessed many more gene copy numbers of alkaline phosphatase and thioredoxin reductase.
32941125	10	35	from	52909T	1028:1033	arg1	content					998:1004	G+C content	994:1004	G+C content of 67.0 mol% in SCSIO 52909T and of 4.42 Mbp	994:1049	Genome sequencing revealed two large genomes of 4.58 Mbp with G+C content of 67.0 mol% in SCSIO 52909T and of 4.42 Mbp with G+C content of 69.1 % in SCSIO 52915T.
32941125	6	36	theme	Optimal	497:503	arg1	growth					505:510	Optimal growth	497:510	Optimal growth	497:510	Optimal growth occurred at 28 °C, pH 7 and 3% salinity over 14 days cultivation.
32941125	11	37	dep	many	1211:1214	arg1	more					1216:1219	more	1216:1219	more	1216:1219	The two novel strains encoded genes for metabolism that are absent in most other Rubrobacter species, and possessed many more gene copy numbers of alkaline phosphatase and thioredoxin reductase.
32941125	9	38	theme	fatty	845:849	arg1	acids					851:855	Their major fatty acids	833:855	Their major fatty acids	833:855	Their major fatty acids differed, but both contained iso-branched components of C16 : 0 12-methyl.
32941125	0	39	theme	Rubrobacter	0:10	arg1	sp					21:22	Rubrobacter tropicus sp	0:22	Rubrobacter tropicus sp.	0:23	Rubrobacter tropicus sp.
32941125	15	40	theme	strain	1932:1937	arg1	52909T					1945:1950	the type strain SCSIO 52909T	1923:1950	the type strain SCSIO 52909T (=KCTC 49412T=CGMCC 1.13853T)	1923:1980	nov., with the type strain SCSIO 52909T (=KCTC 49412T=CGMCC 1.13853T), and Rubrobacter marinus sp.
32941125	15	40	theme	strain	1932:1937	arg1	1.13853T					1972:1979	=KCTC 49412T=CGMCC 1.13853T	1953:1979	=KCTC 49412T=CGMCC 1.13853T	1953:1979	nov., with the type strain SCSIO 52909T (=KCTC 49412T=CGMCC 1.13853T), and Rubrobacter marinus sp.
32941125	11	41	theme	other	1170:1174	arg1	species					1188:1194	most other Rubrobacter species	1165:1194	most other Rubrobacter species	1165:1194	The two novel strains encoded genes for metabolism that are absent in most other Rubrobacter species, and possessed many more gene copy numbers of alkaline phosphatase and thioredoxin reductase.
32941125	14	42	from	species	1833:1839	arg1	Rubrobacter					1854:1864	the genus Rubrobacter	1844:1864	the genus Rubrobacter	1844:1864	Based on the data presented here, these two strains should be recognized as two new species in the genus Rubrobacter, for which the names Rubrobacter tropicus sp.
32941125	12	43	theme	95.0 	1402:1406	arg1	%					1407:1407	%	1407:1407	%	1407:1407	Results of gANI and 16S rRNA gene analyses suggested that the two strains represent two new species, with 74.9, 95.0 % pairwise similarity between each other, and less than 74.3 and 93.5 % to other recognized Rubrobacter species, respectively.
32941125	15	44	theme	Rubrobacter	1987:1997	arg1	sp					2007:2008	Rubrobacter marinus sp	1987:2008	Rubrobacter marinus sp	1987:2008	nov., with the type strain SCSIO 52909T (=KCTC 49412T=CGMCC 1.13853T), and Rubrobacter marinus sp.
32941125	4	45	theme	Phenotypic	313:322	arg1	characteristics					352:366	Phenotypic, chemotaxonomic and genomic characteristics	313:366	Phenotypic, chemotaxonomic and genomic characteristics	313:366	Phenotypic, chemotaxonomic and genomic characteristics were investigated.
32941125	13	46	theme	phylogenetic	1541:1552	arg1	analysis					1554:1561	the phylogenetic analysis	1537:1561	the phylogenetic analysis	1537:1561	In the phylogenetic analysis, strains SCSIO 52909T and SCSIO 52915T were separately clustered together and formed a well-separated phylogenetic branch distinct from the other known species in the genus Rubrobacter.
32941125	11	47	from	species	1188:1194	arg1	absent					1155:1160	absent	1155:1160	absent	1155:1160	The two novel strains encoded genes for metabolism that are absent in most other Rubrobacter species, and possessed many more gene copy numbers of alkaline phosphatase and thioredoxin reductase.
32941125	12	48	theme	pairwise	1409:1416	arg1	similarity					1418:1427	95.0 % pairwise similarity	1402:1427	95.0 % pairwise similarity between each other	1402:1446	Results of gANI and 16S rRNA gene analyses suggested that the two strains represent two new species, with 74.9, 95.0 % pairwise similarity between each other, and less than 74.3 and 93.5 % to other recognized Rubrobacter species, respectively.
32941125	11	49	theme	novel	1103:1107	arg1	genes					1125:1129	genes	1125:1129	genes for metabolism that are absent in most other Rubrobacter species	1125:1194	The two novel strains encoded genes for metabolism that are absent in most other Rubrobacter species, and possessed many more gene copy numbers of alkaline phosphatase and thioredoxin reductase.
32941125	11	49	theme	novel	1103:1107	arg1	strains					1109:1115	The two novel strains	1095:1115	The two novel strains	1095:1115	The two novel strains encoded genes for metabolism that are absent in most other Rubrobacter species, and possessed many more gene copy numbers of alkaline phosphatase and thioredoxin reductase.
32941125	9	50	theme	C16 	913:916	arg1	components					899:908	iso-branched components	886:908	iso-branched components of C16 : 0 12-methyl	886:929	Their major fatty acids differed, but both contained iso-branched components of C16 : 0 12-methyl.
32941125	3	51	attach	isolated	210:217	arg1	sample					244:249	a deep-sea sediment sample	224:249	a deep-sea sediment sample collected at about 3448 m water depth of the South China Sea	224:310	Two novel Gram-stain-positive bacteria, designated as SCSIO 52909T and SCSIO 52915T, were isolated from a deep-sea sediment sample collected at about 3448 m water depth of the South China Sea.
32941125	3	51	attach	isolated	210:217	arg2	bacteria					150:157	Two novel Gram-stain-positive bacteria	120:157	Two novel Gram-stain-positive bacteria	120:157	Two novel Gram-stain-positive bacteria, designated as SCSIO 52909T and SCSIO 52915T, were isolated from a deep-sea sediment sample collected at about 3448 m water depth of the South China Sea.
32941125	9	52	theme	 0	918:919	arg1	12-methyl					921:929	 0 12-methyl	918:929	iso-branched components of C16 : 0 12-methyl	886:929	Their major fatty acids differed, but both contained iso-branched components of C16 : 0 12-methyl.
32941125	2	53	theme	South	103:107	arg1	Sea					115:117	the South China Sea	99:117	the South China Sea	99:117	nov., isolated from deep-sea sediment of the South China Sea.
32941125	13	54	dep	strains	1564:1570	arg1	52915T					1595:1600	52915T	1595:1600	52915T	1595:1600	In the phylogenetic analysis, strains SCSIO 52909T and SCSIO 52915T were separately clustered together and formed a well-separated phylogenetic branch distinct from the other known species in the genus Rubrobacter.
32941125	13	54	dep	strains	1564:1570	arg1	strains					1564:1570	strains	1564:1570	strains SCSIO 52909T and SCSIO 52915T	1564:1600	In the phylogenetic analysis, strains SCSIO 52909T and SCSIO 52915T were separately clustered together and formed a well-separated phylogenetic branch distinct from the other known species in the genus Rubrobacter.
32941125	13	54	dep	strains	1564:1570	arg1	52909T					1578:1583	52909T	1578:1583	52909T	1578:1583	In the phylogenetic analysis, strains SCSIO 52909T and SCSIO 52915T were separately clustered together and formed a well-separated phylogenetic branch distinct from the other known species in the genus Rubrobacter.
32941125	16	55	theme	SCSIO	2038:2042	arg1	52915T					2044:2049	the type strain SCSIO 52915T	2022:2049	the type strain SCSIO 52915T (=KCTC 49411T=CGMCC 1.13852T)	2022:2079	nov., with the type strain SCSIO 52915T (=KCTC 49411T=CGMCC 1.13852T), are proposed.
32941125	16	55	theme	SCSIO	2038:2042	arg1	1.13852T					2071:2078	=KCTC 49411T=CGMCC 1.13852T	2052:2078	=KCTC 49411T=CGMCC 1.13852T	2052:2078	nov., with the type strain SCSIO 52915T (=KCTC 49411T=CGMCC 1.13852T), are proposed.
32941125	13	56	theme	other	1703:1707	arg1	species					1715:1721	the other known species	1699:1721	the other known species in the genus Rubrobacter	1699:1746	In the phylogenetic analysis, strains SCSIO 52909T and SCSIO 52915T were separately clustered together and formed a well-separated phylogenetic branch distinct from the other known species in the genus Rubrobacter.
32941125	10	57	from	52915T	1087:1092	arg1	content					1060:1066	G+C content	1056:1066	G+C content of 69.1 % in SCSIO 52915T	1056:1092	Genome sequencing revealed two large genomes of 4.58 Mbp with G+C content of 67.0 mol% in SCSIO 52909T and of 4.42 Mbp with G+C content of 69.1 % in SCSIO 52915T.
32941125	3	58	dep	m	275:275	arg1	3448					270:273	3448	270:273	3448	270:273	Two novel Gram-stain-positive bacteria, designated as SCSIO 52909T and SCSIO 52915T, were isolated from a deep-sea sediment sample collected at about 3448 m water depth of the South China Sea.
32941125	14	59	theme	new	1829:1831	arg1	species					1833:1839	two new species	1825:1839	two new species	1825:1839	Based on the data presented here, these two strains should be recognized as two new species in the genus Rubrobacter, for which the names Rubrobacter tropicus sp.
32941125	14	59	theme	new	1829:1831	arg1	strains					1793:1799	these two strains	1783:1799	these two strains	1783:1799	Based on the data presented here, these two strains should be recognized as two new species in the genus Rubrobacter, for which the names Rubrobacter tropicus sp.
32941125	12	60	theme	new	1378:1380	arg1	species					1382:1388	two new species	1374:1388	two new species	1374:1388	Results of gANI and 16S rRNA gene analyses suggested that the two strains represent two new species, with 74.9, 95.0 % pairwise similarity between each other, and less than 74.3 and 93.5 % to other recognized Rubrobacter species, respectively.
32941125	3	61	theme	deep-sea	226:233	arg1	sample					244:249	a deep-sea sediment sample	224:249	a deep-sea sediment sample collected at about 3448 m water depth of the South China Sea	224:310	Two novel Gram-stain-positive bacteria, designated as SCSIO 52909T and SCSIO 52915T, were isolated from a deep-sea sediment sample collected at about 3448 m water depth of the South China Sea.
32941125	4	62	theme	chemotaxonomic	325:338	arg1	characteristics					352:366	Phenotypic, chemotaxonomic and genomic characteristics	313:366	Phenotypic, chemotaxonomic and genomic characteristics	313:366	Phenotypic, chemotaxonomic and genomic characteristics were investigated.
32941125	13	63	theme	distinct	1685:1692	arg1	branch					1678:1683	a well-separated phylogenetic branch	1648:1683	a well-separated phylogenetic branch distinct from the other known species in the genus Rubrobacter	1648:1746	In the phylogenetic analysis, strains SCSIO 52909T and SCSIO 52915T were separately clustered together and formed a well-separated phylogenetic branch distinct from the other known species in the genus Rubrobacter.
32941125	11	64	theme	reductase	1279:1287	arg1	numbers					1231:1237	many more gene copy numbers	1211:1237	many more gene copy numbers of alkaline phosphatase and thioredoxin reductase	1211:1287	The two novel strains encoded genes for metabolism that are absent in most other Rubrobacter species, and possessed many more gene copy numbers of alkaline phosphatase and thioredoxin reductase.
32941125	2	65	theme	Sea	115:117	arg1	sediment					87:94	deep-sea sediment	78:94	deep-sea sediment of the South China Sea	78:117	nov., isolated from deep-sea sediment of the South China Sea.
32941125	15	66	theme	49412T=CGMCC	1959:1970	arg1	52909T					1945:1950	the type strain SCSIO 52909T	1923:1950	the type strain SCSIO 52909T (=KCTC 49412T=CGMCC 1.13853T)	1923:1980	nov., with the type strain SCSIO 52909T (=KCTC 49412T=CGMCC 1.13853T), and Rubrobacter marinus sp.
32941125	15	66	theme	49412T=CGMCC	1959:1970	arg1	1.13853T					1972:1979	=KCTC 49412T=CGMCC 1.13853T	1953:1979	=KCTC 49412T=CGMCC 1.13853T	1953:1979	nov., with the type strain SCSIO 52909T (=KCTC 49412T=CGMCC 1.13853T), and Rubrobacter marinus sp.
32941125	4	67	theme	genomic	344:350	arg1	characteristics					352:366	Phenotypic, chemotaxonomic and genomic characteristics	313:366	Phenotypic, chemotaxonomic and genomic characteristics	313:366	Phenotypic, chemotaxonomic and genomic characteristics were investigated.
32941125	12	68	theme	16S	1310:1312	arg1	rRNA					1314:1317	16S rRNA	1310:1317	16S rRNA	1310:1317	Results of gANI and 16S rRNA gene analyses suggested that the two strains represent two new species, with 74.9, 95.0 % pairwise similarity between each other, and less than 74.3 and 93.5 % to other recognized Rubrobacter species, respectively.
32941125	16	69	with	nov.	2011:2014	arg1	52915T					2044:2049	the type strain SCSIO 52915T	2022:2049	the type strain SCSIO 52915T (=KCTC 49411T=CGMCC 1.13852T)	2022:2079	nov., with the type strain SCSIO 52915T (=KCTC 49411T=CGMCC 1.13852T), are proposed.
32941125	16	69	with	nov.	2011:2014	arg1	1.13852T					2071:2078	=KCTC 49411T=CGMCC 1.13852T	2052:2078	=KCTC 49411T=CGMCC 1.13852T	2052:2078	nov., with the type strain SCSIO 52915T (=KCTC 49411T=CGMCC 1.13852T), are proposed.
32941125	12	70	theme	gene	1319:1322	arg1	analyses					1324:1331	gene analyses	1319:1331	gene analyses	1319:1331	Results of gANI and 16S rRNA gene analyses suggested that the two strains represent two new species, with 74.9, 95.0 % pairwise similarity between each other, and less than 74.3 and 93.5 % to other recognized Rubrobacter species, respectively.
32941125	6	71	theme	%	541:541	arg1	salinity					543:550	3% salinity	540:550	3% salinity	540:550	Optimal growth occurred at 28 °C, pH 7 and 3% salinity over 14 days cultivation.
32941125	10	72	theme	67.0 mol	1009:1016	arg1	%					1017:1017	67.0 mol%	1009:1017	67.0 mol% in SCSIO 52909T	1009:1033	Genome sequencing revealed two large genomes of 4.58 Mbp with G+C content of 67.0 mol% in SCSIO 52909T and of 4.42 Mbp with G+C content of 69.1 % in SCSIO 52915T.
32941125	5	73	theme	oxidase	457:463	arg1	activity					465:472	oxidase activity	457:472	oxidase activity	457:472	These strains were aerobic and tested positive for catalase activity, oxidase activity and nitrate reduction.
32941125	15	74	with	nov.	1912:1915	arg1	52909T					1945:1950	the type strain SCSIO 52909T	1923:1950	the type strain SCSIO 52909T (=KCTC 49412T=CGMCC 1.13853T)	1923:1980	nov., with the type strain SCSIO 52909T (=KCTC 49412T=CGMCC 1.13853T), and Rubrobacter marinus sp.
32941125	15	74	with	nov.	1912:1915	arg1	1.13853T					1972:1979	=KCTC 49412T=CGMCC 1.13853T	1953:1979	=KCTC 49412T=CGMCC 1.13853T	1953:1979	nov., with the type strain SCSIO 52909T (=KCTC 49412T=CGMCC 1.13853T), and Rubrobacter marinus sp.
32941125	12	75	theme	other	1482:1486	arg1	species					1511:1517	other recognized Rubrobacter species	1482:1517	other recognized Rubrobacter species	1482:1517	Results of gANI and 16S rRNA gene analyses suggested that the two strains represent two new species, with 74.9, 95.0 % pairwise similarity between each other, and less than 74.3 and 93.5 % to other recognized Rubrobacter species, respectively.
32941125	13	76	theme	well-separated	1650:1663	arg1	branch					1678:1683	a well-separated phylogenetic branch	1648:1683	a well-separated phylogenetic branch distinct from the other known species in the genus Rubrobacter	1648:1746	In the phylogenetic analysis, strains SCSIO 52909T and SCSIO 52915T were separately clustered together and formed a well-separated phylogenetic branch distinct from the other known species in the genus Rubrobacter.
32941125	2	77	theme	deep-sea	78:85	arg1	sediment					87:94	deep-sea sediment	78:94	deep-sea sediment of the South China Sea	78:117	nov., isolated from deep-sea sediment of the South China Sea.
32941125	12	78	theme	Rubrobacter	1499:1509	arg1	species					1511:1517	other recognized Rubrobacter species	1482:1517	other recognized Rubrobacter species	1482:1517	Results of gANI and 16S rRNA gene analyses suggested that the two strains represent two new species, with 74.9, 95.0 % pairwise similarity between each other, and less than 74.3 and 93.5 % to other recognized Rubrobacter species, respectively.
32941125	3	79	theme	water	277:281	arg1	depth					283:287	about 3448 m water depth	264:287	about 3448 m water depth of the South China Sea	264:310	Two novel Gram-stain-positive bacteria, designated as SCSIO 52909T and SCSIO 52915T, were isolated from a deep-sea sediment sample collected at about 3448 m water depth of the South China Sea.
32941125	12	80	theme	gANI	1301:1304	arg1	Results					1290:1296	Results	1290:1296	Results of gANI and 16S rRNA gene analyses	1290:1331	Results of gANI and 16S rRNA gene analyses suggested that the two strains represent two new species, with 74.9, 95.0 % pairwise similarity between each other, and less than 74.3 and 93.5 % to other recognized Rubrobacter species, respectively.
32941125	1	81	theme	nov.	25:28	arg1	marinus					46:52	nov. and Rubrobacter marinus	25:52	nov. and Rubrobacter marinus	25:52	nov. and Rubrobacter marinus sp.
32941125	14	82	theme	genus	1848:1852	arg1	Rubrobacter					1854:1864	the genus Rubrobacter	1844:1864	the genus Rubrobacter	1844:1864	Based on the data presented here, these two strains should be recognized as two new species in the genus Rubrobacter, for which the names Rubrobacter tropicus sp.
32941125	3	83	theme	South	296:300	arg1	Sea					308:310	the South China Sea	292:310	the South China Sea	292:310	Two novel Gram-stain-positive bacteria, designated as SCSIO 52909T and SCSIO 52915T, were isolated from a deep-sea sediment sample collected at about 3448 m water depth of the South China Sea.
32941125	10	84	from	%	1017:1017	arg1	52909T					1028:1033	SCSIO 52909T	1022:1033	SCSIO 52909T	1022:1033	Genome sequencing revealed two large genomes of 4.58 Mbp with G+C content of 67.0 mol% in SCSIO 52909T and of 4.42 Mbp with G+C content of 69.1 % in SCSIO 52915T.
32941125	10	85	theme	Mbp	1047:1049	arg1	content					998:1004	G+C content	994:1004	G+C content of 67.0 mol% in SCSIO 52909T and of 4.42 Mbp	994:1049	Genome sequencing revealed two large genomes of 4.58 Mbp with G+C content of 67.0 mol% in SCSIO 52909T and of 4.42 Mbp with G+C content of 69.1 % in SCSIO 52915T.
32941125	11	86	theme	copy	1226:1229	arg1	numbers					1231:1237	many more gene copy numbers	1211:1237	many more gene copy numbers of alkaline phosphatase and thioredoxin reductase	1211:1287	The two novel strains encoded genes for metabolism that are absent in most other Rubrobacter species, and possessed many more gene copy numbers of alkaline phosphatase and thioredoxin reductase.
32941125	13	87	from	species	1715:1721	arg1	distinct					1685:1692	distinct	1685:1692	distinct	1685:1692	In the phylogenetic analysis, strains SCSIO 52909T and SCSIO 52915T were separately clustered together and formed a well-separated phylogenetic branch distinct from the other known species in the genus Rubrobacter.
32941125	13	87	from	species	1715:1721	arg1	Rubrobacter					1736:1746	the genus Rubrobacter	1726:1746	the genus Rubrobacter	1726:1746	In the phylogenetic analysis, strains SCSIO 52909T and SCSIO 52915T were separately clustered together and formed a well-separated phylogenetic branch distinct from the other known species in the genus Rubrobacter.
32941125	7	88	theme	type	610:613	arg1	l-Lys-l-Ala					620:630	l-Lys-l-Ala	620:630	l-Lys-l-Ala	620:630	Its peptidoglycan structure was type A3α (l-Lys-l-Ala) and the only menaquinone was MK-8.
32941125	7	88	theme	type	610:613	arg1	A3α					615:617	type A3α	610:617	type A3α (l-Lys-l-Ala)	610:631	Its peptidoglycan structure was type A3α (l-Lys-l-Ala) and the only menaquinone was MK-8.
32941125	3	89	theme	Gram-stain-positive	130:148	arg1	bacteria					150:157	Two novel Gram-stain-positive bacteria	120:157	Two novel Gram-stain-positive bacteria	120:157	Two novel Gram-stain-positive bacteria, designated as SCSIO 52909T and SCSIO 52915T, were isolated from a deep-sea sediment sample collected at about 3448 m water depth of the South China Sea.
32941125	15	90	theme	SCSIO	1939:1943	arg1	52909T					1945:1950	the type strain SCSIO 52909T	1923:1950	the type strain SCSIO 52909T (=KCTC 49412T=CGMCC 1.13853T)	1923:1980	nov., with the type strain SCSIO 52909T (=KCTC 49412T=CGMCC 1.13853T), and Rubrobacter marinus sp.
32941125	15	90	theme	SCSIO	1939:1943	arg1	1.13853T					1972:1979	=KCTC 49412T=CGMCC 1.13853T	1953:1979	=KCTC 49412T=CGMCC 1.13853T	1953:1979	nov., with the type strain SCSIO 52909T (=KCTC 49412T=CGMCC 1.13853T), and Rubrobacter marinus sp.
32941125	16	91	theme	strain	2031:2036	arg1	52915T					2044:2049	the type strain SCSIO 52915T	2022:2049	the type strain SCSIO 52915T (=KCTC 49411T=CGMCC 1.13852T)	2022:2079	nov., with the type strain SCSIO 52915T (=KCTC 49411T=CGMCC 1.13852T), are proposed.
32941125	16	91	theme	strain	2031:2036	arg1	1.13852T					2071:2078	=KCTC 49411T=CGMCC 1.13852T	2052:2078	=KCTC 49411T=CGMCC 1.13852T	2052:2078	nov., with the type strain SCSIO 52915T (=KCTC 49411T=CGMCC 1.13852T), are proposed.
32941125	3	92	theme	Sea	308:310	arg1	depth					283:287	about 3448 m water depth	264:287	about 3448 m water depth of the South China Sea	264:310	Two novel Gram-stain-positive bacteria, designated as SCSIO 52909T and SCSIO 52915T, were isolated from a deep-sea sediment sample collected at about 3448 m water depth of the South China Sea.
32941125	11	93	theme	phosphatase	1251:1261	arg1	numbers					1231:1237	many more gene copy numbers	1211:1237	many more gene copy numbers of alkaline phosphatase and thioredoxin reductase	1211:1287	The two novel strains encoded genes for metabolism that are absent in most other Rubrobacter species, and possessed many more gene copy numbers of alkaline phosphatase and thioredoxin reductase.
32941125	0	94	theme	tropicus	12:19	arg1	sp					21:22	Rubrobacter tropicus sp	0:22	Rubrobacter tropicus sp.	0:23	Rubrobacter tropicus sp.
32941125	10	95	theme	G+C	1056:1058	arg1	content					1060:1066	G+C content	1056:1066	G+C content of 69.1 % in SCSIO 52915T	1056:1092	Genome sequencing revealed two large genomes of 4.58 Mbp with G+C content of 67.0 mol% in SCSIO 52909T and of 4.42 Mbp with G+C content of 69.1 % in SCSIO 52915T.
32941125	14	96	theme	tropicus	1899:1906	arg1	sp					1908:1909	tropicus sp	1899:1909	tropicus sp	1899:1909	Based on the data presented here, these two strains should be recognized as two new species in the genus Rubrobacter, for which the names Rubrobacter tropicus sp.
32941125	10	97	theme	SCSIO	1081:1085	arg1	52915T					1087:1092	SCSIO 52915T	1081:1092	SCSIO 52915T	1081:1092	Genome sequencing revealed two large genomes of 4.58 Mbp with G+C content of 67.0 mol% in SCSIO 52909T and of 4.42 Mbp with G+C content of 69.1 % in SCSIO 52915T.
32941125	10	98	theme	Genome	932:937	arg1	sequencing					939:948	Genome sequencing	932:948	Genome sequencing	932:948	Genome sequencing revealed two large genomes of 4.58 Mbp with G+C content of 67.0 mol% in SCSIO 52909T and of 4.42 Mbp with G+C content of 69.1 % in SCSIO 52915T.
32941125	11	99	theme	Rubrobacter	1176:1186	arg1	species					1188:1194	most other Rubrobacter species	1165:1194	most other Rubrobacter species	1165:1194	The two novel strains encoded genes for metabolism that are absent in most other Rubrobacter species, and possessed many more gene copy numbers of alkaline phosphatase and thioredoxin reductase.
32941125	12	100	dep	gANI	1301:1304	arg1	analyses					1324:1331	gene analyses	1319:1331	gene analyses	1319:1331	Results of gANI and 16S rRNA gene analyses suggested that the two strains represent two new species, with 74.9, 95.0 % pairwise similarity between each other, and less than 74.3 and 93.5 % to other recognized Rubrobacter species, respectively.
32941125	10	101	from	content	1060:1066	arg1	52915T					1087:1092	SCSIO 52915T	1081:1092	SCSIO 52915T	1081:1092	Genome sequencing revealed two large genomes of 4.58 Mbp with G+C content of 67.0 mol% in SCSIO 52909T and of 4.42 Mbp with G+C content of 69.1 % in SCSIO 52915T.
32941125	10	102	theme	%	1076:1076	arg1	content					1060:1066	G+C content	1056:1066	G+C content of 69.1 % in SCSIO 52915T	1056:1092	Genome sequencing revealed two large genomes of 4.58 Mbp with G+C content of 67.0 mol% in SCSIO 52909T and of 4.42 Mbp with G+C content of 69.1 % in SCSIO 52915T.
32941125	15	103	theme	marinus	1999:2005	arg1	sp					2007:2008	Rubrobacter marinus sp	1987:2008	Rubrobacter marinus sp	1987:2008	nov., with the type strain SCSIO 52909T (=KCTC 49412T=CGMCC 1.13853T), and Rubrobacter marinus sp.
32941125	11	104	theme	most	1165:1168	arg1	species					1188:1194	most other Rubrobacter species	1165:1194	most other Rubrobacter species	1165:1194	The two novel strains encoded genes for metabolism that are absent in most other Rubrobacter species, and possessed many more gene copy numbers of alkaline phosphatase and thioredoxin reductase.
32941125	16	105	theme	type	2026:2029	arg1	52915T					2044:2049	the type strain SCSIO 52915T	2022:2049	the type strain SCSIO 52915T (=KCTC 49411T=CGMCC 1.13852T)	2022:2079	nov., with the type strain SCSIO 52915T (=KCTC 49411T=CGMCC 1.13852T), are proposed.
32941125	16	105	theme	type	2026:2029	arg1	1.13852T					2071:2078	=KCTC 49411T=CGMCC 1.13852T	2052:2078	=KCTC 49411T=CGMCC 1.13852T	2052:2078	nov., with the type strain SCSIO 52915T (=KCTC 49411T=CGMCC 1.13852T), are proposed.
32941125	10	106	theme	large	963:967	arg1	genomes					969:975	two large genomes	959:975	two large genomes of 4.58 Mbp	959:987	Genome sequencing revealed two large genomes of 4.58 Mbp with G+C content of 67.0 mol% in SCSIO 52909T and of 4.42 Mbp with G+C content of 69.1 % in SCSIO 52915T.
32941125	9	107	theme	iso-branched	886:897	arg1	components					899:908	iso-branched components	886:908	iso-branched components of C16 : 0 12-methyl	886:929	Their major fatty acids differed, but both contained iso-branched components of C16 : 0 12-methyl.
32941125	9	108	theme	major	839:843	arg1	acids					851:855	Their major fatty acids	833:855	Their major fatty acids	833:855	Their major fatty acids differed, but both contained iso-branched components of C16 : 0 12-methyl.
32941125	10	109	theme	G+C	994:996	arg1	content					998:1004	G+C content	994:1004	G+C content of 67.0 mol% in SCSIO 52909T and of 4.42 Mbp	994:1049	Genome sequencing revealed two large genomes of 4.58 Mbp with G+C content of 67.0 mol% in SCSIO 52909T and of 4.42 Mbp with G+C content of 69.1 % in SCSIO 52915T.
32941125	11	110	contain	possessed	1201:1209	arg1	genes					1125:1129	genes	1125:1129	genes for metabolism that are absent in most other Rubrobacter species	1125:1194	The two novel strains encoded genes for metabolism that are absent in most other Rubrobacter species, and possessed many more gene copy numbers of alkaline phosphatase and thioredoxin reductase.
32941125	11	110	contain	possessed	1201:1209	arg1	strains					1109:1115	The two novel strains	1095:1115	The two novel strains	1095:1115	The two novel strains encoded genes for metabolism that are absent in most other Rubrobacter species, and possessed many more gene copy numbers of alkaline phosphatase and thioredoxin reductase.
32941125	11	110	contain	possessed	1201:1209	arg2	numbers					1231:1237	many more gene copy numbers	1211:1237	many more gene copy numbers of alkaline phosphatase and thioredoxin reductase	1211:1287	The two novel strains encoded genes for metabolism that are absent in most other Rubrobacter species, and possessed many more gene copy numbers of alkaline phosphatase and thioredoxin reductase.
32941125	12	111	theme	%	1407:1407	arg1	similarity					1418:1427	95.0 % pairwise similarity	1402:1427	95.0 % pairwise similarity between each other	1402:1446	Results of gANI and 16S rRNA gene analyses suggested that the two strains represent two new species, with 74.9, 95.0 % pairwise similarity between each other, and less than 74.3 and 93.5 % to other recognized Rubrobacter species, respectively.
32941125	10	112	theme	Mbp	985:987	arg1	genomes					969:975	two large genomes	959:975	two large genomes of 4.58 Mbp	959:987	Genome sequencing revealed two large genomes of 4.58 Mbp with G+C content of 67.0 mol% in SCSIO 52909T and of 4.42 Mbp with G+C content of 69.1 % in SCSIO 52915T.
32941125	8	113	contain	possessed	681:689	arg2	glycolipid					788:797	an unidentified glycolipid	772:797	an unidentified glycolipid	772:797	Both strains possessed diphosphatidylglycerol, phosphatidylglycerol, an unidentified phosphoglycolipid, an unidentified glycolipid and an unidentified phospholipid.
32941125	8	113	contain	possessed	681:689	arg2	diphosphatidylglycerol					691:712	diphosphatidylglycerol	691:712	diphosphatidylglycerol	691:712	Both strains possessed diphosphatidylglycerol, phosphatidylglycerol, an unidentified phosphoglycolipid, an unidentified glycolipid and an unidentified phospholipid.
32941125	8	113	contain	possessed	681:689	arg2	phosphoglycolipid					753:769	an unidentified phosphoglycolipid	737:769	an unidentified phosphoglycolipid	737:769	Both strains possessed diphosphatidylglycerol, phosphatidylglycerol, an unidentified phosphoglycolipid, an unidentified glycolipid and an unidentified phospholipid.
32941125	8	113	contain	possessed	681:689	arg1	strains					673:679	Both strains	668:679	Both strains	668:679	Both strains possessed diphosphatidylglycerol, phosphatidylglycerol, an unidentified phosphoglycolipid, an unidentified glycolipid and an unidentified phospholipid.
32941125	8	113	contain	possessed	681:689	arg2	phosphatidylglycerol					715:734	phosphatidylglycerol	715:734	phosphatidylglycerol	715:734	Both strains possessed diphosphatidylglycerol, phosphatidylglycerol, an unidentified phosphoglycolipid, an unidentified glycolipid and an unidentified phospholipid.
32941125	8	113	contain	possessed	681:689	arg2	phospholipid					819:830	an unidentified phospholipid	803:830	an unidentified phospholipid	803:830	Both strains possessed diphosphatidylglycerol, phosphatidylglycerol, an unidentified phosphoglycolipid, an unidentified glycolipid and an unidentified phospholipid.
32941125	13	114	theme	genus	1730:1734	arg1	Rubrobacter					1736:1746	the genus Rubrobacter	1726:1746	the genus Rubrobacter	1726:1746	In the phylogenetic analysis, strains SCSIO 52909T and SCSIO 52915T were separately clustered together and formed a well-separated phylogenetic branch distinct from the other known species in the genus Rubrobacter.
32941125	2	115	theme	China	109:113	arg1	Sea					115:117	the South China Sea	99:117	the South China Sea	99:117	nov., isolated from deep-sea sediment of the South China Sea.
32502215	7	0	theme	Most	1103:1106	arg1	parameters					1108:1117	Most parameters	1103:1117	Most parameters	1103:1117	Most parameters changed with age or differed with the piglet's birth weight.
32502215	12	1	used	used	1862:1865	arg2	qPCR					1783:1786	real-time qPCR	1773:1786	real-time qPCR	1773:1786	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	12	1	used	used	1862:1865	arg2	density					1704:1710	The density	1700:1710	The density of intra-epithelial lymphocytes	1700:1742	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	12	1	used	used	1862:1865	arg2	profiles					1763:1770	the expression profiles	1748:1770	the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ)	1748:1855	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	15	2	theme	action	2180:2185	arg1	modes					2171:2175	the modes	2167:2175	the modes of action for these effects	2167:2203	However, the modes of action for these effects are not yet fully elucidated and its potential to improve other performance parameters needs further investigation.
32502215	1	3	theme	litter	274:279	arg1	resilience					281:290	lower litter resilience	268:290	lower litter resilience	268:290	Farmers face difficulties in redeeming their investment in larger litter sizes since this comes with larger litter heterogenicity, lower litter resilience and risk of higher mortality.
32502215	6	4	theme	immune	1063:1068	arg1	system					1070:1075	mucosal immune system	1055:1075	mucosal immune system	1055:1075	Additional parameters reflecting gut health (intestinal integrity and morphology, mucosal immune system) were analysed at d 36.5.
32502215	5	5	theme	digesta	878:884	arg1	profile					857:863	profile	857:863	profile of feces and digesta	857:884	Performance parameters, gut microbiome and short-chain fatty acids profile of feces and digesta were assessed at birth (d 0), d 7, weaning (d 21.5) and 2 weeks post-weaning (d 36.5).
32502215	4	6	theme	age	785:787	arg1	days					777:780	7 or 21 days	769:780	7 or 21 days of age	769:787	Therefore, this field trial studied the effect of dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation to low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth until 7 or 21 days of age.
32502215	1	7	theme	larger	196:201	arg1	sizes					210:214	larger litter sizes	196:214	larger litter sizes since this comes with larger litter heterogenicity, lower litter resilience and risk of higher mortality	196:319	Farmers face difficulties in redeeming their investment in larger litter sizes since this comes with larger litter heterogenicity, lower litter resilience and risk of higher mortality.
32502215	14	8	theme	body	1989:1992	arg1	weight					1994:1999	The improved body weight	1976:1999	The improved body weight	1976:1999	The improved body weight and the reduced post-weaning mortality seen in piglets supplemented with scFOS support the view that scFOS positively impact piglet's health and resilience.
32502215	6	9	theme	intestinal	1018:1027	arg1	integrity					1029:1037	intestinal integrity	1018:1037	intestinal integrity	1018:1037	Additional parameters reflecting gut health (intestinal integrity and morphology, mucosal immune system) were analysed at d 36.5.
32502215	6	9	theme	intestinal	1018:1027	arg1	health					1010:1015	gut health	1006:1015	gut health (intestinal integrity and morphology, mucosal immune system)	1006:1076	Additional parameters reflecting gut health (intestinal integrity and morphology, mucosal immune system) were analysed at d 36.5.
32502215	11	10	theme	crypt	1644:1648	arg1	lenght					1636:1641	villus lenght	1629:1641	villus lenght	1629:1641	Also, intestinal architecture (villus lenght, crypt depth) was not affected by scFOS supplementation.
32502215	11	10	theme	crypt	1644:1648	arg1	depth					1650:1654	crypt depth	1644:1654	crypt depth	1644:1654	Also, intestinal architecture (villus lenght, crypt depth) was not affected by scFOS supplementation.
32502215	12	11	theme	system-related	1800:1813	arg1	TNFα					1842:1845	TNFα	1842:1845	TNFα	1842:1845	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	12	11	theme	system-related	1800:1813	arg1	IL-10					1822:1826	IL-10	1822:1826	IL-10	1822:1826	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	12	11	theme	system-related	1800:1813	arg1	IL-1ß					1829:1833	IL-1ß	1829:1833	IL-1ß	1829:1833	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	12	11	theme	system-related	1800:1813	arg1	IFNγ					1851:1854	IFNγ	1851:1854	IFNγ	1851:1854	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	12	11	theme	system-related	1800:1813	arg1	genes					1815:1819	immune system-related genes	1793:1819	immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ)	1793:1855	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	12	11	theme	system-related	1800:1813	arg1	IL-6					1836:1839	IL-6	1836:1839	IL-6	1836:1839	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	9	12	theme	clear	1313:1317	arg1	difference					1319:1328	No clear difference	1310:1328	No clear difference in the IgG level, the microbiota composition and fermentative activity between the treatment groups	1310:1428	No clear difference in the IgG level, the microbiota composition and fermentative activity between the treatment groups was observed.
32502215	15	13	theme	performance	2269:2279	arg1	parameters					2281:2290	other performance parameters	2263:2290	other performance parameters	2263:2290	However, the modes of action for these effects are not yet fully elucidated and its potential to improve other performance parameters needs further investigation.
32502215	4	14	theme	high	704:707	arg1	piglets					744:750	low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets	632:750	low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth	632:761	Therefore, this field trial studied the effect of dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation to low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth until 7 or 21 days of age.
32502215	11	15	theme	intestinal	1604:1613	arg1	architecture					1615:1626	intestinal architecture	1604:1626	intestinal architecture (villus lenght, crypt depth)	1604:1655	Also, intestinal architecture (villus lenght, crypt depth) was not affected by scFOS supplementation.
32502215	9	16	theme	IgG	1337:1339	arg1	level					1341:1345	the IgG level	1333:1345	the IgG level	1333:1345	No clear difference in the IgG level, the microbiota composition and fermentative activity between the treatment groups was observed.
32502215	5	17	theme	short-chain	833:843	arg1	acids					851:855	short-chain fatty acids	833:855	short-chain fatty acids	833:855	Performance parameters, gut microbiome and short-chain fatty acids profile of feces and digesta were assessed at birth (d 0), d 7, weaning (d 21.5) and 2 weeks post-weaning (d 36.5).
32502215	14	18	theme	reduced	2009:2015	arg1	mortality					2030:2038	the reduced post-weaning mortality	2005:2038	the reduced post-weaning mortality seen in piglets supplemented with scFOS	2005:2078	The improved body weight and the reduced post-weaning mortality seen in piglets supplemented with scFOS support the view that scFOS positively impact piglet's health and resilience.
32502215	4	19	theme	field	509:513	arg1	trial					515:519	this field trial	504:519	this field trial	504:519	Therefore, this field trial studied the effect of dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation to low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth until 7 or 21 days of age.
32502215	4	20	theme	intermediate	676:687	arg1	NBW					667:669	NBW	667:669	NBW	667:669	Therefore, this field trial studied the effect of dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation to low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth until 7 or 21 days of age.
32502215	4	20	theme	intermediate	676:687	arg1	quartiles					689:697	two intermediate quartiles	672:697	two intermediate quartiles	672:697	Therefore, this field trial studied the effect of dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation to low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth until 7 or 21 days of age.
32502215	13	21	theme	IFNγ	1900:1903	arg1	expression					1905:1914	IFNγ expression	1900:1914	IFNγ expression	1900:1914	Only IFNγ expression, was upregulated in piglets that received scFOS for 7 days.
32502215	12	22	theme	real-time	1773:1781	arg1	profiles					1763:1770	the expression profiles	1748:1770	the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ)	1748:1855	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	12	22	theme	real-time	1773:1781	arg1	qPCR					1783:1786	real-time qPCR	1773:1786	real-time qPCR	1773:1786	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	9	23	theme	microbiota	1352:1361	arg1	composition					1363:1373	the microbiota composition	1348:1373	the microbiota composition	1348:1373	No clear difference in the IgG level, the microbiota composition and fermentative activity between the treatment groups was observed.
32502215	0	24	theme	performance	109:119	arg1	health					129:134	pre- and post-weaning performance and gut health	87:134	pre- and post-weaning performance and gut health	87:134	Short-chain fructo-oligosaccharides supplementation to suckling piglets: Assessment of pre- and post-weaning performance and gut health.
32502215	4	25	theme	weight	737:742	arg1	piglets					744:750	low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets	632:750	low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth	632:761	Therefore, this field trial studied the effect of dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation to low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth until 7 or 21 days of age.
32502215	10	26	theme	intestinal	1505:1514	arg1	permeability					1516:1527	intestinal permeability	1505:1527	intestinal permeability	1505:1527	Additionnally, intestinal integrity, determined by measuring intestinal permeability and regenerative capacity, was similar between the treatment groups.
32502215	0	27	theme	gut	125:127	arg1	health					129:134	pre- and post-weaning performance and gut health	87:134	pre- and post-weaning performance and gut health	87:134	Short-chain fructo-oligosaccharides supplementation to suckling piglets: Assessment of pre- and post-weaning performance and gut health.
32502215	0	28	theme	Short-chain	0:10	arg1	supplementation					36:50	Short-chain fructo-oligosaccharides supplementation	0:50	Short-chain fructo-oligosaccharides supplementation to suckling piglets: Assessment of pre- and post-weaning performance and gut health.	0:135	Short-chain fructo-oligosaccharides supplementation to suckling piglets: Assessment of pre- and post-weaning performance and gut health.
32502215	4	29	theme	fructo-oligosaccharides	563:585	arg1	supplementation					613:627	dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation	543:627	dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation	543:627	Therefore, this field trial studied the effect of dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation to low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth until 7 or 21 days of age.
32502215	4	30	theme	upper	715:719	arg1	HBW					710:712	HBW	710:712	HBW	710:712	Therefore, this field trial studied the effect of dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation to low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth until 7 or 21 days of age.
32502215	4	30	theme	upper	715:719	arg1	quartile					721:728	upper quartile	715:728	upper quartile	715:728	Therefore, this field trial studied the effect of dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation to low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth until 7 or 21 days of age.
32502215	15	31	theme	other	2263:2267	arg1	parameters					2281:2290	other performance parameters	2263:2290	other performance parameters	2263:2290	However, the modes of action for these effects are not yet fully elucidated and its potential to improve other performance parameters needs further investigation.
32502215	1	32	theme	higher	304:309	arg1	mortality					311:319	higher mortality	304:319	higher mortality	304:319	Farmers face difficulties in redeeming their investment in larger litter sizes since this comes with larger litter heterogenicity, lower litter resilience and risk of higher mortality.
32502215	7	33	theme	birth	1166:1170	arg1	weight					1172:1177	the piglet's birth weight	1153:1177	the piglet's birth weight	1153:1177	Most parameters changed with age or differed with the piglet's birth weight.
32502215	8	34	theme	suckling	1238:1245	arg1	piglets					1247:1253	NBW suckling piglets	1234:1253	NBW suckling piglets	1234:1253	Drenching with scFOS increased body weight by 1 kg in NBW suckling piglets and reduced the post-weaning mortality rate by a 100%.
32502215	0	35	theme	suckling	55:62	arg1	piglets					64:70	suckling piglets	55:70	suckling piglets	55:70	Short-chain fructo-oligosaccharides supplementation to suckling piglets: Assessment of pre- and post-weaning performance and gut health.
32502215	12	36	theme	lymphocytes	1732:1742	arg1	profiles					1763:1770	the expression profiles	1748:1770	the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ)	1748:1855	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	12	36	theme	lymphocytes	1732:1742	arg1	qPCR					1783:1786	real-time qPCR	1773:1786	real-time qPCR	1773:1786	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	12	36	theme	lymphocytes	1732:1742	arg1	density					1704:1710	The density	1700:1710	The density of intra-epithelial lymphocytes	1700:1742	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	5	37	dep	parameters	802:811	arg1	profile					857:863	profile	857:863	profile of feces and digesta	857:884	Performance parameters, gut microbiome and short-chain fatty acids profile of feces and digesta were assessed at birth (d 0), d 7, weaning (d 21.5) and 2 weeks post-weaning (d 36.5).
32502215	6	38	theme	gut	1006:1008	arg1	integrity					1029:1037	intestinal integrity	1018:1037	intestinal integrity	1018:1037	Additional parameters reflecting gut health (intestinal integrity and morphology, mucosal immune system) were analysed at d 36.5.
32502215	6	38	theme	gut	1006:1008	arg1	morphology					1043:1052	morphology	1043:1052	morphology	1043:1052	Additional parameters reflecting gut health (intestinal integrity and morphology, mucosal immune system) were analysed at d 36.5.
32502215	6	38	theme	gut	1006:1008	arg1	health					1010:1015	gut health	1006:1015	gut health (intestinal integrity and morphology, mucosal immune system)	1006:1076	Additional parameters reflecting gut health (intestinal integrity and morphology, mucosal immune system) were analysed at d 36.5.
32502215	4	39	theme	dietary	543:549	arg1	supplementation					613:627	dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation	543:627	dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation	543:627	Therefore, this field trial studied the effect of dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation to low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth until 7 or 21 days of age.
32502215	8	40	with	Drenching	1180:1188	arg1	scFOS					1195:1199	scFOS	1195:1199	scFOS	1195:1199	Drenching with scFOS increased body weight by 1 kg in NBW suckling piglets and reduced the post-weaning mortality rate by a 100%.
32502215	1	41	theme	larger	238:243	arg1	heterogenicity					252:265	larger litter heterogenicity	238:265	larger litter heterogenicity	238:265	Farmers face difficulties in redeeming their investment in larger litter sizes since this comes with larger litter heterogenicity, lower litter resilience and risk of higher mortality.
32502215	4	42	dep	normal	659:664	arg1	NBW					667:669	NBW	667:669	NBW	667:669	Therefore, this field trial studied the effect of dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation to low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth until 7 or 21 days of age.
32502215	4	42	dep	normal	659:664	arg1	quartiles					689:697	two intermediate quartiles	672:697	two intermediate quartiles	672:697	Therefore, this field trial studied the effect of dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation to low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth until 7 or 21 days of age.
32502215	4	43	theme	supplementation	613:627	arg1	effect					533:538	the effect	529:538	the effect of dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation to low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth	529:761	Therefore, this field trial studied the effect of dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation to low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth until 7 or 21 days of age.
32502215	8	44	theme	mortality	1284:1292	arg1	rate					1294:1297	the post-weaning mortality rate	1267:1297	the post-weaning mortality rate	1267:1297	Drenching with scFOS increased body weight by 1 kg in NBW suckling piglets and reduced the post-weaning mortality rate by a 100%.
32502215	4	45	theme	low	632:634	arg1	piglets					744:750	low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets	632:750	low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth	632:761	Therefore, this field trial studied the effect of dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation to low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth until 7 or 21 days of age.
32502215	10	46	theme	treatment	1580:1588	arg1	groups					1590:1595	the treatment groups	1576:1595	the treatment groups	1576:1595	Additionnally, intestinal integrity, determined by measuring intestinal permeability and regenerative capacity, was similar between the treatment groups.
32502215	8	47	from	kg	1228:1229	arg1	piglets					1247:1253	NBW suckling piglets	1234:1253	NBW suckling piglets	1234:1253	Drenching with scFOS increased body weight by 1 kg in NBW suckling piglets and reduced the post-weaning mortality rate by a 100%.
32502215	1	48	theme	lower	268:272	arg1	resilience					281:290	lower litter resilience	268:290	lower litter resilience	268:290	Farmers face difficulties in redeeming their investment in larger litter sizes since this comes with larger litter heterogenicity, lower litter resilience and risk of higher mortality.
32502215	14	49	theme	post-weaning	2017:2028	arg1	mortality					2030:2038	the reduced post-weaning mortality	2005:2038	the reduced post-weaning mortality seen in piglets supplemented with scFOS	2005:2078	The improved body weight and the reduced post-weaning mortality seen in piglets supplemented with scFOS support the view that scFOS positively impact piglet's health and resilience.
32502215	6	50	theme	mucosal	1055:1061	arg1	system					1070:1075	mucosal immune system	1055:1075	mucosal immune system	1055:1075	Additional parameters reflecting gut health (intestinal integrity and morphology, mucosal immune system) were analysed at d 36.5.
32502215	1	51	theme	litter	203:208	arg1	sizes					210:214	larger litter sizes	196:214	larger litter sizes since this comes with larger litter heterogenicity, lower litter resilience and risk of higher mortality	196:319	Farmers face difficulties in redeeming their investment in larger litter sizes since this comes with larger litter heterogenicity, lower litter resilience and risk of higher mortality.
32502215	5	52	theme	gut	814:816	arg1	microbiome					818:827	gut microbiome	814:827	gut microbiome	814:827	Performance parameters, gut microbiome and short-chain fatty acids profile of feces and digesta were assessed at birth (d 0), d 7, weaning (d 21.5) and 2 weeks post-weaning (d 36.5).
32502215	9	53	from	difference	1319:1328	arg1	composition					1363:1373	the microbiota composition	1348:1373	the microbiota composition	1348:1373	No clear difference in the IgG level, the microbiota composition and fermentative activity between the treatment groups was observed.
32502215	9	53	from	difference	1319:1328	arg1	activity					1392:1399	fermentative activity	1379:1399	fermentative activity	1379:1399	No clear difference in the IgG level, the microbiota composition and fermentative activity between the treatment groups was observed.
32502215	9	53	from	difference	1319:1328	arg1	level					1341:1345	the IgG level	1333:1345	the IgG level	1333:1345	No clear difference in the IgG level, the microbiota composition and fermentative activity between the treatment groups was observed.
32502215	4	54	theme	normal	659:664	arg1	piglets					744:750	low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets	632:750	low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth	632:761	Therefore, this field trial studied the effect of dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation to low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth until 7 or 21 days of age.
32502215	14	55	theme	improved	1980:1987	arg1	weight					1994:1999	The improved body weight	1976:1999	The improved body weight	1976:1999	The improved body weight and the reduced post-weaning mortality seen in piglets supplemented with scFOS support the view that scFOS positively impact piglet's health and resilience.
32502215	2	56	theme	Dietary	322:328	arg1	oligosaccharides					330:345	Dietary oligosaccharides	322:345	Dietary oligosaccharides	322:345	Dietary oligosaccharides, given to the sow, proved beneficial for the offspring's performance.
32502215	5	57	theme	post-weaning	950:961	arg1	weeks					944:948	2 weeks post-weaning	942:961	2 weeks post-weaning (d 36.5)	942:970	Performance parameters, gut microbiome and short-chain fatty acids profile of feces and digesta were assessed at birth (d 0), d 7, weaning (d 21.5) and 2 weeks post-weaning (d 36.5).
32502215	5	57	theme	post-weaning	950:961	arg1	d					964:964	d 36.5	964:969	d 36.5	964:969	Performance parameters, gut microbiome and short-chain fatty acids profile of feces and digesta were assessed at birth (d 0), d 7, weaning (d 21.5) and 2 weeks post-weaning (d 36.5).
32502215	6	58	dep	integrity	1029:1037	arg1	system					1070:1075	mucosal immune system	1055:1075	mucosal immune system	1055:1075	Additional parameters reflecting gut health (intestinal integrity and morphology, mucosal immune system) were analysed at d 36.5.
32502215	12	59	theme	immune	1793:1798	arg1	TNFα					1842:1845	TNFα	1842:1845	TNFα	1842:1845	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	12	59	theme	immune	1793:1798	arg1	IL-10					1822:1826	IL-10	1822:1826	IL-10	1822:1826	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	12	59	theme	immune	1793:1798	arg1	IL-1ß					1829:1833	IL-1ß	1829:1833	IL-1ß	1829:1833	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	12	59	theme	immune	1793:1798	arg1	IFNγ					1851:1854	IFNγ	1851:1854	IFNγ	1851:1854	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	12	59	theme	immune	1793:1798	arg1	genes					1815:1819	immune system-related genes	1793:1819	immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ)	1793:1855	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	12	59	theme	immune	1793:1798	arg1	IL-6					1836:1839	IL-6	1836:1839	IL-6	1836:1839	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	4	60	theme	lower	642:646	arg1	LBW					637:639	LBW	637:639	LBW	637:639	Therefore, this field trial studied the effect of dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation to low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth until 7 or 21 days of age.
32502215	4	60	theme	lower	642:646	arg1	quartile					648:655	lower quartile	642:655	lower quartile	642:655	Therefore, this field trial studied the effect of dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation to low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth until 7 or 21 days of age.
32502215	10	61	theme	intestinal	1459:1468	arg1	integrity					1470:1478	intestinal integrity	1459:1478	intestinal integrity	1459:1478	Additionnally, intestinal integrity, determined by measuring intestinal permeability and regenerative capacity, was similar between the treatment groups.
32502215	11	62	theme	scFOS	1677:1681	arg1	supplementation					1683:1697	scFOS supplementation	1677:1697	scFOS supplementation	1677:1697	Also, intestinal architecture (villus lenght, crypt depth) was not affected by scFOS supplementation.
32502215	11	63	dep	architecture	1615:1626	arg1	lenght					1636:1641	villus lenght	1629:1641	villus lenght	1629:1641	Also, intestinal architecture (villus lenght, crypt depth) was not affected by scFOS supplementation.
32502215	11	63	dep	architecture	1615:1626	arg1	depth					1650:1654	crypt depth	1644:1654	crypt depth	1644:1654	Also, intestinal architecture (villus lenght, crypt depth) was not affected by scFOS supplementation.
32502215	15	64	theme	further	2298:2304	arg1	investigation					2306:2318	further investigation	2298:2318	further investigation	2298:2318	However, the modes of action for these effects are not yet fully elucidated and its potential to improve other performance parameters needs further investigation.
32502215	4	65	dep	low	632:634	arg1	LBW					637:639	LBW	637:639	LBW	637:639	Therefore, this field trial studied the effect of dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation to low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth until 7 or 21 days of age.
32502215	4	65	dep	low	632:634	arg1	quartile					648:655	lower quartile	642:655	lower quartile	642:655	Therefore, this field trial studied the effect of dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation to low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth until 7 or 21 days of age.
32502215	5	66	theme	feces	868:872	arg1	profile					857:863	profile	857:863	profile of feces and digesta	857:884	Performance parameters, gut microbiome and short-chain fatty acids profile of feces and digesta were assessed at birth (d 0), d 7, weaning (d 21.5) and 2 weeks post-weaning (d 36.5).
32502215	0	67	theme	pre-	87:90	arg1	health					129:134	pre- and post-weaning performance and gut health	87:134	pre- and post-weaning performance and gut health	87:134	Short-chain fructo-oligosaccharides supplementation to suckling piglets: Assessment of pre- and post-weaning performance and gut health.
32502215	4	68	from	birth	757:761	arg1	piglets					744:750	low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets	632:750	low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth	632:761	Therefore, this field trial studied the effect of dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation to low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth until 7 or 21 days of age.
32502215	0	69	theme	post-weaning	96:107	arg1	health					129:134	pre- and post-weaning performance and gut health	87:134	pre- and post-weaning performance and gut health	87:134	Short-chain fructo-oligosaccharides supplementation to suckling piglets: Assessment of pre- and post-weaning performance and gut health.
32502215	5	70	theme	fatty	845:849	arg1	acids					851:855	short-chain fatty acids	833:855	short-chain fatty acids	833:855	Performance parameters, gut microbiome and short-chain fatty acids profile of feces and digesta were assessed at birth (d 0), d 7, weaning (d 21.5) and 2 weeks post-weaning (d 36.5).
32502215	12	71	dep	genes	1815:1819	arg1	TNFα					1842:1845	TNFα	1842:1845	TNFα	1842:1845	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	12	71	dep	genes	1815:1819	arg1	IL-10					1822:1826	IL-10	1822:1826	IL-10	1822:1826	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	12	71	dep	genes	1815:1819	arg1	IL-1ß					1829:1833	IL-1ß	1829:1833	IL-1ß	1829:1833	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	12	71	dep	genes	1815:1819	arg1	IFNγ					1851:1854	IFNγ	1851:1854	IFNγ	1851:1854	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	12	71	dep	genes	1815:1819	arg1	genes					1815:1819	immune system-related genes	1793:1819	immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ)	1793:1855	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	12	71	dep	genes	1815:1819	arg1	IL-6					1836:1839	IL-6	1836:1839	IL-6	1836:1839	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	12	72	theme	mucosal	1877:1883	arg1	immunity					1885:1892	mucosal immunity	1877:1892	mucosal immunity	1877:1892	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	0	73	dep	supplementation	36:50	arg1	Assessment					73:82	Assessment	73:82	Short-chain fructo-oligosaccharides supplementation to suckling piglets: Assessment of pre- and post-weaning performance and gut health.	0:135	Short-chain fructo-oligosaccharides supplementation to suckling piglets: Assessment of pre- and post-weaning performance and gut health.
32502215	1	74	from	investment	182:191	arg1	sizes					210:214	larger litter sizes	196:214	larger litter sizes since this comes with larger litter heterogenicity, lower litter resilience and risk of higher mortality	196:319	Farmers face difficulties in redeeming their investment in larger litter sizes since this comes with larger litter heterogenicity, lower litter resilience and risk of higher mortality.
32502215	11	75	theme	villus	1629:1634	arg1	lenght					1636:1641	villus lenght	1629:1641	villus lenght	1629:1641	Also, intestinal architecture (villus lenght, crypt depth) was not affected by scFOS supplementation.
32502215	11	75	theme	villus	1629:1634	arg1	depth					1650:1654	crypt depth	1644:1654	crypt depth	1644:1654	Also, intestinal architecture (villus lenght, crypt depth) was not affected by scFOS supplementation.
32502215	0	76	theme	health	129:134	arg1	Assessment					73:82	Assessment	73:82	Short-chain fructo-oligosaccharides supplementation to suckling piglets: Assessment of pre- and post-weaning performance and gut health.	0:135	Short-chain fructo-oligosaccharides supplementation to suckling piglets: Assessment of pre- and post-weaning performance and gut health.
32502215	4	77	dep	scFOS	588:592	arg1	drenched					603:610	drenched	603:610	drenched	603:610	Therefore, this field trial studied the effect of dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation to low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth until 7 or 21 days of age.
32502215	4	77	dep	scFOS	588:592	arg1	1g/day					595:600	1g/day	595:600	scFOS; 1g/day; drenched	588:610	Therefore, this field trial studied the effect of dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation to low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth until 7 or 21 days of age.
32502215	12	78	theme	expression	1752:1761	arg1	profiles					1763:1770	the expression profiles	1748:1770	the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ)	1748:1855	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	12	78	theme	expression	1752:1761	arg1	qPCR					1783:1786	real-time qPCR	1773:1786	real-time qPCR	1773:1786	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	9	79	theme	fermentative	1379:1390	arg1	activity					1392:1399	fermentative activity	1379:1399	fermentative activity	1379:1399	No clear difference in the IgG level, the microbiota composition and fermentative activity between the treatment groups was observed.
32502215	4	80	theme	birth	731:735	arg1	piglets					744:750	low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets	632:750	low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth	632:761	Therefore, this field trial studied the effect of dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation to low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth until 7 or 21 days of age.
32502215	3	81	theme	suckling	457:464	arg1	piglet					466:471	the suckling piglet	453:471	the suckling piglet	453:471	However, giving oligosaccharides to the suckling piglet is poorly explored.
32502215	0	82	theme	fructo-oligosaccharides	12:34	arg1	supplementation					36:50	Short-chain fructo-oligosaccharides supplementation	0:50	Short-chain fructo-oligosaccharides supplementation to suckling piglets: Assessment of pre- and post-weaning performance and gut health.	0:135	Short-chain fructo-oligosaccharides supplementation to suckling piglets: Assessment of pre- and post-weaning performance and gut health.
32502215	4	83	dep	fructo-oligosaccharides	563:585	arg1	scFOS					588:592	scFOS	588:592	scFOS; 1g/day; drenched	588:610	Therefore, this field trial studied the effect of dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation to low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth until 7 or 21 days of age.
32502215	4	84	dep	high	704:707	arg1	HBW					710:712	HBW	710:712	HBW	710:712	Therefore, this field trial studied the effect of dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation to low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth until 7 or 21 days of age.
32502215	4	84	dep	high	704:707	arg1	quartile					721:728	upper quartile	715:728	upper quartile	715:728	Therefore, this field trial studied the effect of dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation to low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth until 7 or 21 days of age.
32502215	12	85	theme	intra-epithelial	1715:1730	arg1	lymphocytes					1732:1742	intra-epithelial lymphocytes	1715:1742	intra-epithelial lymphocytes	1715:1742	The density of intra-epithelial lymphocytes and the expression profiles (real-time qPCR) for immune system-related genes (IL-10, IL-1ß, IL-6, TNFα and IFNγ) were used to assess mucosal immunity.
32502215	9	86	theme	treatment	1413:1421	arg1	groups					1423:1428	the treatment groups	1409:1428	the treatment groups	1409:1428	No clear difference in the IgG level, the microbiota composition and fermentative activity between the treatment groups was observed.
32502215	10	87	theme	regenerative	1533:1544	arg1	capacity					1546:1553	regenerative capacity	1533:1553	regenerative capacity	1533:1553	Additionnally, intestinal integrity, determined by measuring intestinal permeability and regenerative capacity, was similar between the treatment groups.
32502215	1	88	theme	mortality	311:319	arg1	heterogenicity					252:265	larger litter heterogenicity	238:265	larger litter heterogenicity	238:265	Farmers face difficulties in redeeming their investment in larger litter sizes since this comes with larger litter heterogenicity, lower litter resilience and risk of higher mortality.
32502215	1	88	theme	mortality	311:319	arg1	risk					296:299	risk	296:299	risk of higher mortality	296:319	Farmers face difficulties in redeeming their investment in larger litter sizes since this comes with larger litter heterogenicity, lower litter resilience and risk of higher mortality.
32502215	1	88	theme	mortality	311:319	arg1	resilience					281:290	lower litter resilience	268:290	lower litter resilience	268:290	Farmers face difficulties in redeeming their investment in larger litter sizes since this comes with larger litter heterogenicity, lower litter resilience and risk of higher mortality.
32502215	4	89	theme	short-chain	551:561	arg1	fructo-oligosaccharides					563:585	short-chain fructo-oligosaccharides	551:585	dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation	543:627	Therefore, this field trial studied the effect of dietary short-chain fructo-oligosaccharides (scFOS; 1g/day; drenched) supplementation to low (LBW, lower quartile), normal (NBW, two intermediate quartiles) and high (HBW, upper quartile) birth weight piglets from birth until 7 or 21 days of age.
32502215	8	90	theme	body	1211:1214	arg1	weight					1216:1221	body weight	1211:1221	body weight	1211:1221	Drenching with scFOS increased body weight by 1 kg in NBW suckling piglets and reduced the post-weaning mortality rate by a 100%.
32502215	1	91	theme	litter	245:250	arg1	heterogenicity					252:265	larger litter heterogenicity	238:265	larger litter heterogenicity	238:265	Farmers face difficulties in redeeming their investment in larger litter sizes since this comes with larger litter heterogenicity, lower litter resilience and risk of higher mortality.
32502215	5	92	theme	Performance	790:800	arg1	parameters					802:811	Performance parameters	790:811	Performance parameters	790:811	Performance parameters, gut microbiome and short-chain fatty acids profile of feces and digesta were assessed at birth (d 0), d 7, weaning (d 21.5) and 2 weeks post-weaning (d 36.5).
32502215	6	93	theme	Additional	973:982	arg1	parameters					984:993	Additional parameters	973:993	Additional parameters reflecting gut health (intestinal integrity and morphology, mucosal immune system)	973:1076	Additional parameters reflecting gut health (intestinal integrity and morphology, mucosal immune system) were analysed at d 36.5.
32502215	8	94	theme	post-weaning	1271:1282	arg1	rate					1294:1297	the post-weaning mortality rate	1267:1297	the post-weaning mortality rate	1267:1297	Drenching with scFOS increased body weight by 1 kg in NBW suckling piglets and reduced the post-weaning mortality rate by a 100%.
32195646	10	0	theme	DSM	1227:1229	arg1	5731T					1231:1235	V. fluvialis DSM 5731T	1214:1235	V. fluvialis DSM 5731T	1214:1235	Digital DNA-DNA hybridization of strain CF-49T with V. fluvialis DSM 5731T, V. elongatus CCUG 51432Tand V. lutrae CCUG 39187T resulted in relatedness values of 21.4, 23.3 and 24.6 %, respectively.
32195646	10	0	theme	DSM	1227:1229	arg1	39187T					1281:1286	39187T	1281:1286	39187T	1281:1286	Digital DNA-DNA hybridization of strain CF-49T with V. fluvialis DSM 5731T, V. elongatus CCUG 51432Tand V. lutrae CCUG 39187T resulted in relatedness values of 21.4, 23.3 and 24.6 %, respectively.
32195646	2	1	theme	Gram-stain-positive	108:126	arg1	bacterium					155:163	A Gram-stain-positive, coccus-shaped, non-motile bacterium	106:163	A Gram-stain-positive, coccus-shaped, non-motile bacterium	106:163	A Gram-stain-positive, coccus-shaped, non-motile bacterium, designated CF-49T, was isolated from the cloacal content of a snow finch, which was incidentally captured in a plateau pika burrow on the Qinghai-Tibet Plateau, PR China.
32195646	4	2	theme	NaCl	723:726	arg1	presence					699:706	the presence	695:706	the presence of 0.5 % (w/v) NaCl	695:726	Strains CF-49T and CF-210 grew optimally at 37 °C and pH 7.0 and in the presence of 0.5 % (w/v) NaCl.
32195646	10	3	theme	strain	1195:1200	arg1	CF-49T					1202:1207	strain CF-49T	1195:1207	strain CF-49T	1195:1207	Digital DNA-DNA hybridization of strain CF-49T with V. fluvialis DSM 5731T, V. elongatus CCUG 51432Tand V. lutrae CCUG 39187T resulted in relatedness values of 21.4, 23.3 and 24.6 %, respectively.
32195646	3	4	theme	96.5 	459:463	arg1	51432T					451:456	Vagococcus elongatus CCUG 51432T	425:456	Vagococcus elongatus CCUG 51432T (96.5 % similarity)	425:476	Analysis of the 16S rRNA gene sequence showed that strain CF-49T was closely related to Vagococcus elongatus CCUG 51432T (96.5 % similarity), Vagococcus fluvialis NCFB 2497T (96.0 %) and Vagococcus lutrae CCUG 39187T (95.9 %), whereas the similarity to another isolate (CF-210) was 99.9 %.
32195646	3	4	theme	96.5 	459:463	arg1	similarity					466:475	96.5 % similarity	459:475	96.5 % similarity	459:475	Analysis of the 16S rRNA gene sequence showed that strain CF-49T was closely related to Vagococcus elongatus CCUG 51432T (96.5 % similarity), Vagococcus fluvialis NCFB 2497T (96.0 %) and Vagococcus lutrae CCUG 39187T (95.9 %), whereas the similarity to another isolate (CF-210) was 99.9 %.
32195646	10	5	theme	fluvialis	1217:1225	arg1	5731T					1231:1235	V. fluvialis DSM 5731T	1214:1235	V. fluvialis DSM 5731T	1214:1235	Digital DNA-DNA hybridization of strain CF-49T with V. fluvialis DSM 5731T, V. elongatus CCUG 51432Tand V. lutrae CCUG 39187T resulted in relatedness values of 21.4, 23.3 and 24.6 %, respectively.
32195646	10	5	theme	fluvialis	1217:1225	arg1	39187T					1281:1286	39187T	1281:1286	39187T	1281:1286	Digital DNA-DNA hybridization of strain CF-49T with V. fluvialis DSM 5731T, V. elongatus CCUG 51432Tand V. lutrae CCUG 39187T resulted in relatedness values of 21.4, 23.3 and 24.6 %, respectively.
32195646	9	6	theme	DNA	1143:1145	arg1	%					1159:1159	35.9 mol%	1151:1159	35.9 mol%	1151:1159	The G+C content of the genomic DNA was 35.9 mol%.
32195646	9	6	theme	DNA	1143:1145	arg1	content					1120:1126	The G+C content	1112:1126	The G+C content of the genomic DNA	1112:1145	The G+C content of the genomic DNA was 35.9 mol%.
32195646	10	7	theme	%	1342:1342	arg1	values					1312:1317	relatedness values	1300:1317	relatedness values	1300:1317	Digital DNA-DNA hybridization of strain CF-49T with V. fluvialis DSM 5731T, V. elongatus CCUG 51432Tand V. lutrae CCUG 39187T resulted in relatedness values of 21.4, 23.3 and 24.6 %, respectively.
32195646	3	8	theme	%	464:464	arg1	51432T					451:456	Vagococcus elongatus CCUG 51432T	425:456	Vagococcus elongatus CCUG 51432T (96.5 % similarity)	425:476	Analysis of the 16S rRNA gene sequence showed that strain CF-49T was closely related to Vagococcus elongatus CCUG 51432T (96.5 % similarity), Vagococcus fluvialis NCFB 2497T (96.0 %) and Vagococcus lutrae CCUG 39187T (95.9 %), whereas the similarity to another isolate (CF-210) was 99.9 %.
32195646	3	8	theme	%	464:464	arg1	similarity					466:475	96.5 % similarity	459:475	96.5 % similarity	459:475	Analysis of the 16S rRNA gene sequence showed that strain CF-49T was closely related to Vagococcus elongatus CCUG 51432T (96.5 % similarity), Vagococcus fluvialis NCFB 2497T (96.0 %) and Vagococcus lutrae CCUG 39187T (95.9 %), whereas the similarity to another isolate (CF-210) was 99.9 %.
32195646	2	9	from	burrow	290:295	arg1	China					330:334	the Qinghai-Tibet Plateau, PR China	300:334	China	330:334	A Gram-stain-positive, coccus-shaped, non-motile bacterium, designated CF-49T, was isolated from the cloacal content of a snow finch, which was incidentally captured in a plateau pika burrow on the Qinghai-Tibet Plateau, PR China.
32195646	12	10	theme	1.6436T=GDMCC	1559:1571	arg1	CF-49T					1544:1549	CF-49T	1544:1549	CF-49T (=CGMCC 1.6436T=GDMCC 1.1588T=JCM 33477T)	1544:1591	The type strain is CF-49T (=CGMCC 1.6436T=GDMCC 1.1588T=JCM 33477T).
32195646	12	10	theme	1.6436T=GDMCC	1559:1571	arg1	33477T					1585:1590	=CGMCC 1.6436T=GDMCC 1.1588T=JCM 33477T	1552:1590	=CGMCC 1.6436T=GDMCC 1.1588T=JCM 33477T	1552:1590	The type strain is CF-49T (=CGMCC 1.6436T=GDMCC 1.1588T=JCM 33477T).
32195646	3	11	theme	16S	353:355	arg1	rRNA					357:360	the 16S rRNA	349:360	the 16S rRNA gene sequence	349:374	Analysis of the 16S rRNA gene sequence showed that strain CF-49T was closely related to Vagococcus elongatus CCUG 51432T (96.5 % similarity), Vagococcus fluvialis NCFB 2497T (96.0 %) and Vagococcus lutrae CCUG 39187T (95.9 %), whereas the similarity to another isolate (CF-210) was 99.9 %.
32195646	3	12	theme	fluvialis	490:498	arg1	2497T					505:509	Vagococcus fluvialis NCFB 2497T	479:509	Vagococcus fluvialis NCFB 2497T (96.0 %)	479:518	Analysis of the 16S rRNA gene sequence showed that strain CF-49T was closely related to Vagococcus elongatus CCUG 51432T (96.5 % similarity), Vagococcus fluvialis NCFB 2497T (96.0 %) and Vagococcus lutrae CCUG 39187T (95.9 %), whereas the similarity to another isolate (CF-210) was 99.9 %.
32195646	3	12	theme	fluvialis	490:498	arg1	%					517:517	96.0 %	512:517	96.0 %	512:517	Analysis of the 16S rRNA gene sequence showed that strain CF-49T was closely related to Vagococcus elongatus CCUG 51432T (96.5 % similarity), Vagococcus fluvialis NCFB 2497T (96.0 %) and Vagococcus lutrae CCUG 39187T (95.9 %), whereas the similarity to another isolate (CF-210) was 99.9 %.
32195646	8	13	theme	menaquinone	1085:1095	arg1	quinone					1073:1079	The predominant respiratory quinone	1045:1079	The predominant respiratory quinone	1045:1079	The predominant respiratory quinone was menaquinone MK-7 (68.8 %).
32195646	8	13	theme	menaquinone	1085:1095	arg1	MK-7					1097:1100	menaquinone MK-7	1085:1100	menaquinone MK-7	1085:1100	The predominant respiratory quinone was menaquinone MK-7 (68.8 %).
32195646	9	14	theme	G+C	1116:1118	arg1	%					1159:1159	35.9 mol%	1151:1159	35.9 mol%	1151:1159	The G+C content of the genomic DNA was 35.9 mol%.
32195646	9	14	theme	G+C	1116:1118	arg1	content					1120:1126	The G+C content	1112:1126	The G+C content of the genomic DNA	1112:1145	The G+C content of the genomic DNA was 35.9 mol%.
32195646	8	15	theme	predominant	1049:1059	arg1	quinone					1073:1079	The predominant respiratory quinone	1045:1079	The predominant respiratory quinone	1045:1079	The predominant respiratory quinone was menaquinone MK-7 (68.8 %).
32195646	8	15	theme	predominant	1049:1059	arg1	MK-7					1097:1100	menaquinone MK-7	1085:1100	menaquinone MK-7	1085:1100	The predominant respiratory quinone was menaquinone MK-7 (68.8 %).
32195646	3	16	theme	NCFB	500:503	arg1	2497T					505:509	Vagococcus fluvialis NCFB 2497T	479:509	Vagococcus fluvialis NCFB 2497T (96.0 %)	479:518	Analysis of the 16S rRNA gene sequence showed that strain CF-49T was closely related to Vagococcus elongatus CCUG 51432T (96.5 % similarity), Vagococcus fluvialis NCFB 2497T (96.0 %) and Vagococcus lutrae CCUG 39187T (95.9 %), whereas the similarity to another isolate (CF-210) was 99.9 %.
32195646	3	16	theme	NCFB	500:503	arg1	%					517:517	96.0 %	512:517	96.0 %	512:517	Analysis of the 16S rRNA gene sequence showed that strain CF-49T was closely related to Vagococcus elongatus CCUG 51432T (96.5 % similarity), Vagococcus fluvialis NCFB 2497T (96.0 %) and Vagococcus lutrae CCUG 39187T (95.9 %), whereas the similarity to another isolate (CF-210) was 99.9 %.
32195646	1	17	attach	isolated	34:41	arg2	nov.					28:31	nov.	28:31	nov.	28:31	nov., isolated from snow finch (Montifringilla taczanowskii) cloacal content.
32195646	1	17	attach	isolated	34:41	arg1	finch					53:57	snow finch	48:57	snow finch	48:57	nov., isolated from snow finch (Montifringilla taczanowskii) cloacal content.
32195646	11	18	theme	name	1495:1498	arg1	xieshaowenii					1511:1522	the name Vagococcus xieshaowenii	1491:1522	the name Vagococcus xieshaowenii	1491:1522	Based on results from polyphasic analyses, our two isolates are proposed to represent a novel species in the genus Vagococcus, with the name Vagococcus xieshaowenii.
32195646	4	19	theme	%	715:715	arg1	NaCl					723:726	0.5 % (w/v) NaCl	711:726	0.5 % (w/v) NaCl	711:726	Strains CF-49T and CF-210 grew optimally at 37 °C and pH 7.0 and in the presence of 0.5 % (w/v) NaCl.
32195646	9	20	theme	35.9 mol	1151:1158	arg1	%					1159:1159	35.9 mol%	1151:1159	35.9 mol%	1151:1159	The G+C content of the genomic DNA was 35.9 mol%.
32195646	9	20	theme	35.9 mol	1151:1158	arg1	content					1120:1126	The G+C content	1112:1126	The G+C content of the genomic DNA	1112:1145	The G+C content of the genomic DNA was 35.9 mol%.
32195646	8	21	theme	respiratory	1061:1071	arg1	quinone					1073:1079	The predominant respiratory quinone	1045:1079	The predominant respiratory quinone	1045:1079	The predominant respiratory quinone was menaquinone MK-7 (68.8 %).
32195646	8	21	theme	respiratory	1061:1071	arg1	MK-7					1097:1100	menaquinone MK-7	1085:1100	menaquinone MK-7	1085:1100	The predominant respiratory quinone was menaquinone MK-7 (68.8 %).
32195646	7	22	dep	C16 	962:965	arg1	 0					967:968	 0	967:968	 0	967:968	The major cellular fatty acids (>10 %) were C16 : 0 (35.6 %), C14 : 0 (17.3 %), C18 : 1 ω9c (16.2 %) and C16 : 1 ω9c (10.6 %).
32195646	7	22	dep	C16 	962:965	arg1	%					976:976	35.6 %	971:976	35.6 %	971:976	The major cellular fatty acids (>10 %) were C16 : 0 (35.6 %), C14 : 0 (17.3 %), C18 : 1 ω9c (16.2 %) and C16 : 1 ω9c (10.6 %).
32195646	7	22	dep	C16 	962:965	arg1	 1 ω9c					1028:1033	 1 ω9c	1028:1033	 1 ω9c	1028:1033	The major cellular fatty acids (>10 %) were C16 : 0 (35.6 %), C14 : 0 (17.3 %), C18 : 1 ω9c (16.2 %) and C16 : 1 ω9c (10.6 %).
32195646	7	22	dep	C16 	962:965	arg1	%					1041:1041	10.6 %	1036:1041	10.6 %	1036:1041	The major cellular fatty acids (>10 %) were C16 : 0 (35.6 %), C14 : 0 (17.3 %), C18 : 1 ω9c (16.2 %) and C16 : 1 ω9c (10.6 %).
32195646	7	23	theme	fatty	937:941	arg1	acids					943:947	The major cellular fatty acids	918:947	The major cellular fatty acids (>10 %)	918:955	The major cellular fatty acids (>10 %) were C16 : 0 (35.6 %), C14 : 0 (17.3 %), C18 : 1 ω9c (16.2 %) and C16 : 1 ω9c (10.6 %).
32195646	7	23	theme	fatty	937:941	arg1	%					954:954	>10 %	950:954	>10 %	950:954	The major cellular fatty acids (>10 %) were C16 : 0 (35.6 %), C14 : 0 (17.3 %), C18 : 1 ω9c (16.2 %) and C16 : 1 ω9c (10.6 %).
32195646	7	23	theme	fatty	937:941	arg1	C16 					962:965	C16 	962:965	C16 	962:965	The major cellular fatty acids (>10 %) were C16 : 0 (35.6 %), C14 : 0 (17.3 %), C18 : 1 ω9c (16.2 %) and C16 : 1 ω9c (10.6 %).
32195646	12	24	theme	type	1529:1532	arg1	CF-49T					1544:1549	CF-49T	1544:1549	CF-49T (=CGMCC 1.6436T=GDMCC 1.1588T=JCM 33477T)	1544:1591	The type strain is CF-49T (=CGMCC 1.6436T=GDMCC 1.1588T=JCM 33477T).
32195646	12	24	theme	type	1529:1532	arg1	strain					1534:1539	The type strain	1525:1539	The type strain	1525:1539	The type strain is CF-49T (=CGMCC 1.6436T=GDMCC 1.1588T=JCM 33477T).
32195646	4	25	theme	0.5 	711:714	arg1	%					715:715	%	715:715	%	715:715	Strains CF-49T and CF-210 grew optimally at 37 °C and pH 7.0 and in the presence of 0.5 % (w/v) NaCl.
32195646	10	26	theme	Digital	1162:1168	arg1	hybridization					1178:1190	Digital DNA-DNA hybridization	1162:1190	Digital DNA-DNA hybridization of strain CF-49T with V. fluvialis DSM 5731T, V. elongatus CCUG 51432Tand V. lutrae CCUG 39187T	1162:1286	Digital DNA-DNA hybridization of strain CF-49T with V. fluvialis DSM 5731T, V. elongatus CCUG 51432Tand V. lutrae CCUG 39187T resulted in relatedness values of 21.4, 23.3 and 24.6 %, respectively.
32195646	3	27	theme	Vagococcus	479:488	arg1	2497T					505:509	Vagococcus fluvialis NCFB 2497T	479:509	Vagococcus fluvialis NCFB 2497T (96.0 %)	479:518	Analysis of the 16S rRNA gene sequence showed that strain CF-49T was closely related to Vagococcus elongatus CCUG 51432T (96.5 % similarity), Vagococcus fluvialis NCFB 2497T (96.0 %) and Vagococcus lutrae CCUG 39187T (95.9 %), whereas the similarity to another isolate (CF-210) was 99.9 %.
32195646	3	27	theme	Vagococcus	479:488	arg1	%					517:517	96.0 %	512:517	96.0 %	512:517	Analysis of the 16S rRNA gene sequence showed that strain CF-49T was closely related to Vagococcus elongatus CCUG 51432T (96.5 % similarity), Vagococcus fluvialis NCFB 2497T (96.0 %) and Vagococcus lutrae CCUG 39187T (95.9 %), whereas the similarity to another isolate (CF-210) was 99.9 %.
32195646	0	28	theme	xieshaowenii	11:22	arg1	sp					24:25	Vagococcus xieshaowenii sp	0:25	Vagococcus xieshaowenii sp.	0:26	Vagococcus xieshaowenii sp.
32195646	11	29	theme	novel	1447:1451	arg1	species					1453:1459	a novel species	1445:1459	a novel species	1445:1459	Based on results from polyphasic analyses, our two isolates are proposed to represent a novel species in the genus Vagococcus, with the name Vagococcus xieshaowenii.
32195646	7	30	theme	major	922:926	arg1	acids					943:947	The major cellular fatty acids	918:947	The major cellular fatty acids (>10 %)	918:955	The major cellular fatty acids (>10 %) were C16 : 0 (35.6 %), C14 : 0 (17.3 %), C18 : 1 ω9c (16.2 %) and C16 : 1 ω9c (10.6 %).
32195646	7	30	theme	major	922:926	arg1	%					954:954	>10 %	950:954	>10 %	950:954	The major cellular fatty acids (>10 %) were C16 : 0 (35.6 %), C14 : 0 (17.3 %), C18 : 1 ω9c (16.2 %) and C16 : 1 ω9c (10.6 %).
32195646	7	30	theme	major	922:926	arg1	C16 					962:965	C16 	962:965	C16 	962:965	The major cellular fatty acids (>10 %) were C16 : 0 (35.6 %), C14 : 0 (17.3 %), C18 : 1 ω9c (16.2 %) and C16 : 1 ω9c (10.6 %).
32195646	3	31	dep	Vagococcus	524:533	arg1	lutrae					535:540	lutrae	535:540	lutrae	535:540	Analysis of the 16S rRNA gene sequence showed that strain CF-49T was closely related to Vagococcus elongatus CCUG 51432T (96.5 % similarity), Vagococcus fluvialis NCFB 2497T (96.0 %) and Vagococcus lutrae CCUG 39187T (95.9 %), whereas the similarity to another isolate (CF-210) was 99.9 %.
32195646	0	32	theme	Vagococcus	0:9	arg1	sp					24:25	Vagococcus xieshaowenii sp	0:25	Vagococcus xieshaowenii sp.	0:26	Vagococcus xieshaowenii sp.
32195646	7	33	theme	cellular	928:935	arg1	acids					943:947	The major cellular fatty acids	918:947	The major cellular fatty acids (>10 %)	918:955	The major cellular fatty acids (>10 %) were C16 : 0 (35.6 %), C14 : 0 (17.3 %), C18 : 1 ω9c (16.2 %) and C16 : 1 ω9c (10.6 %).
32195646	7	33	theme	cellular	928:935	arg1	%					954:954	>10 %	950:954	>10 %	950:954	The major cellular fatty acids (>10 %) were C16 : 0 (35.6 %), C14 : 0 (17.3 %), C18 : 1 ω9c (16.2 %) and C16 : 1 ω9c (10.6 %).
32195646	7	33	theme	cellular	928:935	arg1	C16 					962:965	C16 	962:965	C16 	962:965	The major cellular fatty acids (>10 %) were C16 : 0 (35.6 %), C14 : 0 (17.3 %), C18 : 1 ω9c (16.2 %) and C16 : 1 ω9c (10.6 %).
32195646	2	34	theme	pika	285:288	arg1	burrow					290:295	a plateau pika burrow	275:295	a plateau pika burrow on the Qinghai-Tibet Plateau, PR China	275:334	A Gram-stain-positive, coccus-shaped, non-motile bacterium, designated CF-49T, was isolated from the cloacal content of a snow finch, which was incidentally captured in a plateau pika burrow on the Qinghai-Tibet Plateau, PR China.
32195646	3	35	theme	strain	388:393	arg1	CF-49T					395:400	strain CF-49T	388:400	strain CF-49T	388:400	Analysis of the 16S rRNA gene sequence showed that strain CF-49T was closely related to Vagococcus elongatus CCUG 51432T (96.5 % similarity), Vagococcus fluvialis NCFB 2497T (96.0 %) and Vagococcus lutrae CCUG 39187T (95.9 %), whereas the similarity to another isolate (CF-210) was 99.9 %.
32195646	4	36	dep	%	715:715	arg1	w/v					718:720	w/v	718:720	w/v	718:720	Strains CF-49T and CF-210 grew optimally at 37 °C and pH 7.0 and in the presence of 0.5 % (w/v) NaCl.
32195646	3	37	theme	CCUG	542:545	arg1	39187T					547:552	Vagococcus lutrae CCUG 39187T	524:552	Vagococcus lutrae CCUG 39187T (95.9 %)	524:561	Analysis of the 16S rRNA gene sequence showed that strain CF-49T was closely related to Vagococcus elongatus CCUG 51432T (96.5 % similarity), Vagococcus fluvialis NCFB 2497T (96.0 %) and Vagococcus lutrae CCUG 39187T (95.9 %), whereas the similarity to another isolate (CF-210) was 99.9 %.
32195646	3	37	theme	CCUG	542:545	arg1	%					560:560	95.9 %	555:560	95.9 %	555:560	Analysis of the 16S rRNA gene sequence showed that strain CF-49T was closely related to Vagococcus elongatus CCUG 51432T (96.5 % similarity), Vagococcus fluvialis NCFB 2497T (96.0 %) and Vagococcus lutrae CCUG 39187T (95.9 %), whereas the similarity to another isolate (CF-210) was 99.9 %.
32195646	2	38	theme	plateau	277:283	arg1	burrow					290:295	a plateau pika burrow	275:295	a plateau pika burrow on the Qinghai-Tibet Plateau, PR China	275:334	A Gram-stain-positive, coccus-shaped, non-motile bacterium, designated CF-49T, was isolated from the cloacal content of a snow finch, which was incidentally captured in a plateau pika burrow on the Qinghai-Tibet Plateau, PR China.
32195646	7	39	dep	C18 	998:1001	arg1	 1 ω9c					1003:1008	 1 ω9c	1003:1008	 1 ω9c	1003:1008	The major cellular fatty acids (>10 %) were C16 : 0 (35.6 %), C14 : 0 (17.3 %), C18 : 1 ω9c (16.2 %) and C16 : 1 ω9c (10.6 %).
32195646	7	39	dep	C18 	998:1001	arg1	%					1016:1016	16.2 %	1011:1016	16.2 %	1011:1016	The major cellular fatty acids (>10 %) were C16 : 0 (35.6 %), C14 : 0 (17.3 %), C18 : 1 ω9c (16.2 %) and C16 : 1 ω9c (10.6 %).
32195646	4	40	dep	Strains	627:633	arg1	Strains					627:633	Strains CF-49T and CF-210	627:651	Strains CF-49T and CF-210	627:651	Strains CF-49T and CF-210 grew optimally at 37 °C and pH 7.0 and in the presence of 0.5 % (w/v) NaCl.
32195646	4	40	dep	Strains	627:633	arg1	CF-210					646:651	CF-210	646:651	CF-210	646:651	Strains CF-49T and CF-210 grew optimally at 37 °C and pH 7.0 and in the presence of 0.5 % (w/v) NaCl.
32195646	4	40	dep	Strains	627:633	arg1	CF-49T					635:640	CF-49T	635:640	CF-49T	635:640	Strains CF-49T and CF-210 grew optimally at 37 °C and pH 7.0 and in the presence of 0.5 % (w/v) NaCl.
32195646	11	41	from	analyses	1392:1399	arg1	results					1368:1374	results	1368:1374	results from polyphasic analyses	1368:1399	Based on results from polyphasic analyses, our two isolates are proposed to represent a novel species in the genus Vagococcus, with the name Vagococcus xieshaowenii.
32195646	3	42	theme	Vagococcus	425:434	arg1	51432T					451:456	Vagococcus elongatus CCUG 51432T	425:456	Vagococcus elongatus CCUG 51432T (96.5 % similarity)	425:476	Analysis of the 16S rRNA gene sequence showed that strain CF-49T was closely related to Vagococcus elongatus CCUG 51432T (96.5 % similarity), Vagococcus fluvialis NCFB 2497T (96.0 %) and Vagococcus lutrae CCUG 39187T (95.9 %), whereas the similarity to another isolate (CF-210) was 99.9 %.
32195646	3	42	theme	Vagococcus	425:434	arg1	similarity					466:475	96.5 % similarity	459:475	96.5 % similarity	459:475	Analysis of the 16S rRNA gene sequence showed that strain CF-49T was closely related to Vagococcus elongatus CCUG 51432T (96.5 % similarity), Vagococcus fluvialis NCFB 2497T (96.0 %) and Vagococcus lutrae CCUG 39187T (95.9 %), whereas the similarity to another isolate (CF-210) was 99.9 %.
32195646	3	43	theme	sequence	367:374	arg1	Analysis					337:344	Analysis	337:344	Analysis of the 16S rRNA gene sequence	337:374	Analysis of the 16S rRNA gene sequence showed that strain CF-49T was closely related to Vagococcus elongatus CCUG 51432T (96.5 % similarity), Vagococcus fluvialis NCFB 2497T (96.0 %) and Vagococcus lutrae CCUG 39187T (95.9 %), whereas the similarity to another isolate (CF-210) was 99.9 %.
32195646	11	44	theme	Vagococcus	1500:1509	arg1	xieshaowenii					1511:1522	the name Vagococcus xieshaowenii	1491:1522	the name Vagococcus xieshaowenii	1491:1522	Based on results from polyphasic analyses, our two isolates are proposed to represent a novel species in the genus Vagococcus, with the name Vagococcus xieshaowenii.
32195646	10	45	theme	DNA-DNA	1170:1176	arg1	hybridization					1178:1190	Digital DNA-DNA hybridization	1162:1190	Digital DNA-DNA hybridization of strain CF-49T with V. fluvialis DSM 5731T, V. elongatus CCUG 51432Tand V. lutrae CCUG 39187T	1162:1286	Digital DNA-DNA hybridization of strain CF-49T with V. fluvialis DSM 5731T, V. elongatus CCUG 51432Tand V. lutrae CCUG 39187T resulted in relatedness values of 21.4, 23.3 and 24.6 %, respectively.
32195646	3	46	theme	Vagococcus	524:533	arg1	39187T					547:552	Vagococcus lutrae CCUG 39187T	524:552	Vagococcus lutrae CCUG 39187T (95.9 %)	524:561	Analysis of the 16S rRNA gene sequence showed that strain CF-49T was closely related to Vagococcus elongatus CCUG 51432T (96.5 % similarity), Vagococcus fluvialis NCFB 2497T (96.0 %) and Vagococcus lutrae CCUG 39187T (95.9 %), whereas the similarity to another isolate (CF-210) was 99.9 %.
32195646	3	46	theme	Vagococcus	524:533	arg1	%					560:560	95.9 %	555:560	95.9 %	555:560	Analysis of the 16S rRNA gene sequence showed that strain CF-49T was closely related to Vagococcus elongatus CCUG 51432T (96.5 % similarity), Vagococcus fluvialis NCFB 2497T (96.0 %) and Vagococcus lutrae CCUG 39187T (95.9 %), whereas the similarity to another isolate (CF-210) was 99.9 %.
32195646	2	47	attach	isolated	189:196	arg2	bacterium					155:163	A Gram-stain-positive, coccus-shaped, non-motile bacterium	106:163	A Gram-stain-positive, coccus-shaped, non-motile bacterium	106:163	A Gram-stain-positive, coccus-shaped, non-motile bacterium, designated CF-49T, was isolated from the cloacal content of a snow finch, which was incidentally captured in a plateau pika burrow on the Qinghai-Tibet Plateau, PR China.
32195646	2	47	attach	isolated	189:196	arg1	content					215:221	the cloacal content	203:221	the cloacal content of a snow finch, which was incidentally captured in a plateau pika burrow on the Qinghai-Tibet Plateau, PR China	203:334	A Gram-stain-positive, coccus-shaped, non-motile bacterium, designated CF-49T, was isolated from the cloacal content of a snow finch, which was incidentally captured in a plateau pika burrow on the Qinghai-Tibet Plateau, PR China.
32195646	8	48	dep	MK-7	1097:1100	arg1	%					1108:1108	68.8 %	1103:1108	68.8 %	1103:1108	The predominant respiratory quinone was menaquinone MK-7 (68.8 %).
32195646	10	49	theme	relatedness	1300:1310	arg1	values					1312:1317	relatedness values	1300:1317	relatedness values	1300:1317	Digital DNA-DNA hybridization of strain CF-49T with V. fluvialis DSM 5731T, V. elongatus CCUG 51432Tand V. lutrae CCUG 39187T resulted in relatedness values of 21.4, 23.3 and 24.6 %, respectively.
32195646	11	50	theme	genus	1468:1472	arg1	Vagococcus					1474:1483	the genus Vagococcus	1464:1483	the genus Vagococcus	1464:1483	Based on results from polyphasic analyses, our two isolates are proposed to represent a novel species in the genus Vagococcus, with the name Vagococcus xieshaowenii.
32195646	3	51	theme	rRNA	357:360	arg1	sequence					367:374	the 16S rRNA gene sequence	349:374	the 16S rRNA gene sequence	349:374	Analysis of the 16S rRNA gene sequence showed that strain CF-49T was closely related to Vagococcus elongatus CCUG 51432T (96.5 % similarity), Vagococcus fluvialis NCFB 2497T (96.0 %) and Vagococcus lutrae CCUG 39187T (95.9 %), whereas the similarity to another isolate (CF-210) was 99.9 %.
32195646	10	52	theme	V.	1214:1215	arg1	5731T					1231:1235	V. fluvialis DSM 5731T	1214:1235	V. fluvialis DSM 5731T	1214:1235	Digital DNA-DNA hybridization of strain CF-49T with V. fluvialis DSM 5731T, V. elongatus CCUG 51432Tand V. lutrae CCUG 39187T resulted in relatedness values of 21.4, 23.3 and 24.6 %, respectively.
32195646	10	52	theme	V.	1214:1215	arg1	39187T					1281:1286	39187T	1281:1286	39187T	1281:1286	Digital DNA-DNA hybridization of strain CF-49T with V. fluvialis DSM 5731T, V. elongatus CCUG 51432Tand V. lutrae CCUG 39187T resulted in relatedness values of 21.4, 23.3 and 24.6 %, respectively.
32195646	2	53	theme	Plateau	318:324	arg1	China					330:334	the Qinghai-Tibet Plateau, PR China	300:334	China	330:334	A Gram-stain-positive, coccus-shaped, non-motile bacterium, designated CF-49T, was isolated from the cloacal content of a snow finch, which was incidentally captured in a plateau pika burrow on the Qinghai-Tibet Plateau, PR China.
32195646	3	54	theme	gene	362:365	arg1	sequence					367:374	the 16S rRNA gene sequence	349:374	the 16S rRNA gene sequence	349:374	Analysis of the 16S rRNA gene sequence showed that strain CF-49T was closely related to Vagococcus elongatus CCUG 51432T (96.5 % similarity), Vagococcus fluvialis NCFB 2497T (96.0 %) and Vagococcus lutrae CCUG 39187T (95.9 %), whereas the similarity to another isolate (CF-210) was 99.9 %.
32195646	7	55	dep	C14 	980:983	arg1	 0					985:986	 0	985:986	 0	985:986	The major cellular fatty acids (>10 %) were C16 : 0 (35.6 %), C14 : 0 (17.3 %), C18 : 1 ω9c (16.2 %) and C16 : 1 ω9c (10.6 %).
32195646	7	55	dep	C14 	980:983	arg1	%					994:994	17.3 %	989:994	17.3 %	989:994	The major cellular fatty acids (>10 %) were C16 : 0 (35.6 %), C14 : 0 (17.3 %), C18 : 1 ω9c (16.2 %) and C16 : 1 ω9c (10.6 %).
32195646	11	56	theme	polyphasic	1381:1390	arg1	analyses					1392:1399	polyphasic analyses	1381:1399	polyphasic analyses	1381:1399	Based on results from polyphasic analyses, our two isolates are proposed to represent a novel species in the genus Vagococcus, with the name Vagococcus xieshaowenii.
32195646	2	57	theme	cloacal	207:213	arg1	content					215:221	the cloacal content	203:221	the cloacal content of a snow finch, which was incidentally captured in a plateau pika burrow on the Qinghai-Tibet Plateau, PR China	203:334	A Gram-stain-positive, coccus-shaped, non-motile bacterium, designated CF-49T, was isolated from the cloacal content of a snow finch, which was incidentally captured in a plateau pika burrow on the Qinghai-Tibet Plateau, PR China.
32195646	2	58	theme	Qinghai-Tibet	304:316	arg1	China					330:334	the Qinghai-Tibet Plateau, PR China	300:334	China	330:334	A Gram-stain-positive, coccus-shaped, non-motile bacterium, designated CF-49T, was isolated from the cloacal content of a snow finch, which was incidentally captured in a plateau pika burrow on the Qinghai-Tibet Plateau, PR China.
32195646	2	59	dep	Gram-stain-positive	108:126	arg1	non-motile					144:153	non-motile	144:153	non-motile	144:153	A Gram-stain-positive, coccus-shaped, non-motile bacterium, designated CF-49T, was isolated from the cloacal content of a snow finch, which was incidentally captured in a plateau pika burrow on the Qinghai-Tibet Plateau, PR China.
32195646	2	59	dep	Gram-stain-positive	108:126	arg1	coccus-shaped					129:141	coccus-shaped	129:141	coccus-shaped	129:141	A Gram-stain-positive, coccus-shaped, non-motile bacterium, designated CF-49T, was isolated from the cloacal content of a snow finch, which was incidentally captured in a plateau pika burrow on the Qinghai-Tibet Plateau, PR China.
32195646	10	60	theme	CF-49T	1202:1207	arg1	hybridization					1178:1190	Digital DNA-DNA hybridization	1162:1190	Digital DNA-DNA hybridization of strain CF-49T with V. fluvialis DSM 5731T, V. elongatus CCUG 51432Tand V. lutrae CCUG 39187T	1162:1286	Digital DNA-DNA hybridization of strain CF-49T with V. fluvialis DSM 5731T, V. elongatus CCUG 51432Tand V. lutrae CCUG 39187T resulted in relatedness values of 21.4, 23.3 and 24.6 %, respectively.
32195646	3	61	dep	Vagococcus	425:434	arg1	elongatus					436:444	elongatus	436:444	elongatus	436:444	Analysis of the 16S rRNA gene sequence showed that strain CF-49T was closely related to Vagococcus elongatus CCUG 51432T (96.5 % similarity), Vagococcus fluvialis NCFB 2497T (96.0 %) and Vagococcus lutrae CCUG 39187T (95.9 %), whereas the similarity to another isolate (CF-210) was 99.9 %.
32195646	6	62	theme	peptidoglycan	876:888	arg1	A4α					899:901	A4α	899:901	A4α (l-Lys-d-Asp)	899:915	The cell-wall peptidoglycan type was A4α (l-Lys-d-Asp).
32195646	6	62	theme	peptidoglycan	876:888	arg1	type					890:893	The cell-wall peptidoglycan type	862:893	The cell-wall peptidoglycan type	862:893	The cell-wall peptidoglycan type was A4α (l-Lys-d-Asp).
32195646	2	63	dep	China	330:334	arg1	PR					327:328	the Qinghai-Tibet Plateau, PR China	300:334	PR	327:328	A Gram-stain-positive, coccus-shaped, non-motile bacterium, designated CF-49T, was isolated from the cloacal content of a snow finch, which was incidentally captured in a plateau pika burrow on the Qinghai-Tibet Plateau, PR China.
32195646	12	64	theme	1.1588T=JCM	1573:1583	arg1	CF-49T					1544:1549	CF-49T	1544:1549	CF-49T (=CGMCC 1.6436T=GDMCC 1.1588T=JCM 33477T)	1544:1591	The type strain is CF-49T (=CGMCC 1.6436T=GDMCC 1.1588T=JCM 33477T).
32195646	12	64	theme	1.1588T=JCM	1573:1583	arg1	33477T					1585:1590	=CGMCC 1.6436T=GDMCC 1.1588T=JCM 33477T	1552:1590	=CGMCC 1.6436T=GDMCC 1.1588T=JCM 33477T	1552:1590	The type strain is CF-49T (=CGMCC 1.6436T=GDMCC 1.1588T=JCM 33477T).
32195646	1	65	theme	snow	48:51	arg1	finch					53:57	snow finch	48:57	snow finch	48:57	nov., isolated from snow finch (Montifringilla taczanowskii) cloacal content.
32195646	12	66	theme	=CGMCC	1552:1557	arg1	CF-49T					1544:1549	CF-49T	1544:1549	CF-49T (=CGMCC 1.6436T=GDMCC 1.1588T=JCM 33477T)	1544:1591	The type strain is CF-49T (=CGMCC 1.6436T=GDMCC 1.1588T=JCM 33477T).
32195646	12	66	theme	=CGMCC	1552:1557	arg1	33477T					1585:1590	=CGMCC 1.6436T=GDMCC 1.1588T=JCM 33477T	1552:1590	=CGMCC 1.6436T=GDMCC 1.1588T=JCM 33477T	1552:1590	The type strain is CF-49T (=CGMCC 1.6436T=GDMCC 1.1588T=JCM 33477T).
32195646	3	67	theme	CCUG	446:449	arg1	51432T					451:456	Vagococcus elongatus CCUG 51432T	425:456	Vagococcus elongatus CCUG 51432T (96.5 % similarity)	425:476	Analysis of the 16S rRNA gene sequence showed that strain CF-49T was closely related to Vagococcus elongatus CCUG 51432T (96.5 % similarity), Vagococcus fluvialis NCFB 2497T (96.0 %) and Vagococcus lutrae CCUG 39187T (95.9 %), whereas the similarity to another isolate (CF-210) was 99.9 %.
32195646	3	67	theme	CCUG	446:449	arg1	similarity					466:475	96.5 % similarity	459:475	96.5 % similarity	459:475	Analysis of the 16S rRNA gene sequence showed that strain CF-49T was closely related to Vagococcus elongatus CCUG 51432T (96.5 % similarity), Vagococcus fluvialis NCFB 2497T (96.0 %) and Vagococcus lutrae CCUG 39187T (95.9 %), whereas the similarity to another isolate (CF-210) was 99.9 %.
32195646	6	68	theme	cell-wall	866:874	arg1	A4α					899:901	A4α	899:901	A4α (l-Lys-d-Asp)	899:915	The cell-wall peptidoglycan type was A4α (l-Lys-d-Asp).
32195646	6	68	theme	cell-wall	866:874	arg1	type					890:893	The cell-wall peptidoglycan type	862:893	The cell-wall peptidoglycan type	862:893	The cell-wall peptidoglycan type was A4α (l-Lys-d-Asp).
32195646	2	69	theme	finch	233:237	arg1	content					215:221	the cloacal content	203:221	the cloacal content of a snow finch, which was incidentally captured in a plateau pika burrow on the Qinghai-Tibet Plateau, PR China	203:334	A Gram-stain-positive, coccus-shaped, non-motile bacterium, designated CF-49T, was isolated from the cloacal content of a snow finch, which was incidentally captured in a plateau pika burrow on the Qinghai-Tibet Plateau, PR China.
32195646	10	70	with	hybridization	1178:1190	arg1	5731T					1231:1235	V. fluvialis DSM 5731T	1214:1235	V. fluvialis DSM 5731T	1214:1235	Digital DNA-DNA hybridization of strain CF-49T with V. fluvialis DSM 5731T, V. elongatus CCUG 51432Tand V. lutrae CCUG 39187T resulted in relatedness values of 21.4, 23.3 and 24.6 %, respectively.
32195646	10	70	with	hybridization	1178:1190	arg1	39187T					1281:1286	39187T	1281:1286	39187T	1281:1286	Digital DNA-DNA hybridization of strain CF-49T with V. fluvialis DSM 5731T, V. elongatus CCUG 51432Tand V. lutrae CCUG 39187T resulted in relatedness values of 21.4, 23.3 and 24.6 %, respectively.
32195646	9	71	theme	genomic	1135:1141	arg1	DNA					1143:1145	the genomic DNA	1131:1145	the genomic DNA	1131:1145	The G+C content of the genomic DNA was 35.9 mol%.
32195646	2	72	theme	snow	228:231	arg1	finch					233:237	a snow finch	226:237	a snow finch	226:237	A Gram-stain-positive, coccus-shaped, non-motile bacterium, designated CF-49T, was isolated from the cloacal content of a snow finch, which was incidentally captured in a plateau pika burrow on the Qinghai-Tibet Plateau, PR China.
32195646	1	73	dep	content	97:103	arg1	Montifringilla					60:73	Montifringilla	60:73	Montifringilla	60:73	nov., isolated from snow finch (Montifringilla taczanowskii) cloacal content.
32195646	1	73	dep	content	97:103	arg1	cloacal					89:95	cloacal	89:95	cloacal	89:95	nov., isolated from snow finch (Montifringilla taczanowskii) cloacal content.
33103831	1	0	theme	exchange	228:235	arg1	technique					268:276	the chemical exchange saturation transfer (CEST) MRI technique	215:276	the chemical exchange saturation transfer (CEST) MRI technique	215:276	Glucosamine (GlcN) was recently proposed as an agent with an excellent safety profile to detect cancer with the chemical exchange saturation transfer (CEST) MRI technique.
33103831	7	1	theme	sources	1419:1425	arg1	one					1408:1410	one	1408:1410	one	1408:1410	The observation of a significant amount of lactate among the metabolic products hints at acidification as one of the sources of the enhanced CEST effect of GlcN.
33103831	7	1	theme	sources	1419:1425	arg1	sources					1419:1425	the sources	1415:1425	the sources of the enhanced CEST effect of GlcN	1415:1461	The observation of a significant amount of lactate among the metabolic products hints at acidification as one of the sources of the enhanced CEST effect of GlcN.
33103831	6	2	theme	glycosylated	1220:1231	arg1	proteins					1233:1240	the O-linked and N-linked glycosylated proteins	1194:1240	the O-linked and N-linked glycosylated proteins that may be associated with the increase of the NOE signal	1194:1299	The CEST contribution can be attributed to several phosphorylated products of GlcN, including uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc), which is a substrate for the O-linked and N-linked glycosylated proteins that may be associated with the increase of the NOE signal.
33103831	1	3	theme	transfer	248:255	arg1	technique					268:276	the chemical exchange saturation transfer (CEST) MRI technique	215:276	the chemical exchange saturation transfer (CEST) MRI technique	215:276	Glucosamine (GlcN) was recently proposed as an agent with an excellent safety profile to detect cancer with the chemical exchange saturation transfer (CEST) MRI technique.
33103831	7	4	theme	metabolic	1363:1371	arg1	products					1373:1380	the metabolic products	1359:1380	the metabolic products	1359:1380	The observation of a significant amount of lactate among the metabolic products hints at acidification as one of the sources of the enhanced CEST effect of GlcN.
33103831	3	5	theme	Z	496:496	arg1	spectra					498:504	the full Z spectra	487:504	the full Z spectra recorded following GlcN administration to mice bearing implanted 4T1 breast tumors	487:587	Hence, imaging of the GlcN signal was established for the full Z spectra recorded following GlcN administration to mice bearing implanted 4T1 breast tumors.
33103831	5	6	theme	observed	809:816	arg1	effects					823:829	the observed CEST effects	805:829	the observed CEST effects	805:829	The sources of the observed CEST effects were investigated by identifying the GlcN metabolic products as observed by 13 C NMR spectroscopy studies of extracts from the same tumor model following treatment with [UL-13 C] -GlcN·HCl.
33103831	5	7	from	model	969:973	arg1	extracts					940:947	extracts	940:947	extracts from the same tumor model	940:973	The sources of the observed CEST effects were investigated by identifying the GlcN metabolic products as observed by 13 C NMR spectroscopy studies of extracts from the same tumor model following treatment with [UL-13 C] -GlcN·HCl.
33103831	5	7	from	model	969:973	arg1	studies					929:935	13 C NMR spectroscopy studies	907:935	13 C NMR spectroscopy studies of extracts from the same tumor model	907:973	The sources of the observed CEST effects were investigated by identifying the GlcN metabolic products as observed by 13 C NMR spectroscopy studies of extracts from the same tumor model following treatment with [UL-13 C] -GlcN·HCl.
33103831	5	8	theme	effects	823:829	arg1	sources					794:800	The sources	790:800	The sources of the observed CEST effects	790:829	The sources of the observed CEST effects were investigated by identifying the GlcN metabolic products as observed by 13 C NMR spectroscopy studies of extracts from the same tumor model following treatment with [UL-13 C] -GlcN·HCl.
33103831	3	9	theme	GlcN	525:528	arg1	administration					530:543	GlcN administration	525:543	GlcN administration to mice bearing implanted 4T1 breast tumors	525:587	Hence, imaging of the GlcN signal was established for the full Z spectra recorded following GlcN administration to mice bearing implanted 4T1 breast tumors.
33103831	1	10	theme	safety	178:183	arg1	profile					185:191	an excellent safety profile	165:191	an excellent safety profile to detect cancer with the chemical exchange saturation transfer (CEST) MRI technique	165:276	Glucosamine (GlcN) was recently proposed as an agent with an excellent safety profile to detect cancer with the chemical exchange saturation transfer (CEST) MRI technique.
33103831	7	11	theme	significant	1323:1333	arg1	lactate					1345:1351	lactate	1345:1351	lactate	1345:1351	The observation of a significant amount of lactate among the metabolic products hints at acidification as one of the sources of the enhanced CEST effect of GlcN.
33103831	7	11	theme	significant	1323:1333	arg1	amount					1335:1340	a significant amount	1321:1340	a significant amount of lactate	1321:1351	The observation of a significant amount of lactate among the metabolic products hints at acidification as one of the sources of the enhanced CEST effect of GlcN.
33103831	3	12	theme	4T1	571:573	arg1	tumors					582:587	implanted 4T1 breast tumors	561:587	implanted 4T1 breast tumors	561:587	Hence, imaging of the GlcN signal was established for the full Z spectra recorded following GlcN administration to mice bearing implanted 4T1 breast tumors.
33103831	5	13	theme	GlcN	868:871	arg1	products					883:890	the GlcN metabolic products	864:890	the GlcN metabolic products as observed by 13 C NMR spectroscopy studies of extracts from the same tumor model	864:973	The sources of the observed CEST effects were investigated by identifying the GlcN metabolic products as observed by 13 C NMR spectroscopy studies of extracts from the same tumor model following treatment with [UL-13 C] -GlcN·HCl.
33103831	4	14	located	observed	620:627	arg1	ppm					657:659	around 1.5, 3.6 and -3.4 ppm	632:659	around 1.5, 3.6 and -3.4 ppm	632:659	Significant CEST effects were observed at around 1.5, 3.6 and -3.4 ppm, corresponding to the hydroxyl, amine/amide exchangeable protons and for the Nuclear Overhauser Enhancement (NOE), respectively.
33103831	4	14	located	observed	620:627	arg2	effects					607:613	Significant CEST effects	590:613	Significant CEST effects	590:613	Significant CEST effects were observed at around 1.5, 3.6 and -3.4 ppm, corresponding to the hydroxyl, amine/amide exchangeable protons and for the Nuclear Overhauser Enhancement (NOE), respectively.
33103831	0	15	theme	saturation	61:70	arg1	MRI					81:83	glucosamine chemical exchange saturation transfer MRI	31:83	glucosamine chemical exchange saturation transfer MRI	31:83	Molecular imaging of cancer by glucosamine chemical exchange saturation transfer MRI: A preclinical study.
33103831	5	16	theme	NMR	912:914	arg1	studies					929:935	13 C NMR spectroscopy studies	907:935	13 C NMR spectroscopy studies of extracts from the same tumor model	907:973	The sources of the observed CEST effects were investigated by identifying the GlcN metabolic products as observed by 13 C NMR spectroscopy studies of extracts from the same tumor model following treatment with [UL-13 C] -GlcN·HCl.
33103831	6	17	theme	signal	1294:1299	arg1	increase					1274:1281	the increase	1270:1281	the increase of the NOE signal	1270:1299	The CEST contribution can be attributed to several phosphorylated products of GlcN, including uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc), which is a substrate for the O-linked and N-linked glycosylated proteins that may be associated with the increase of the NOE signal.
33103831	2	18	theme	irradiation	420:430	arg1	regions					409:415	the different frequency regions	385:415	the different frequency regions of irradiation	385:430	Translation of the GlcN CEST method to the clinical application requires evaluation of its sensitivity to the different frequency regions of irradiation.
33103831	0	19	theme	Molecular	0:8	arg1	imaging					10:16	Molecular imaging	0:16	Molecular imaging of cancer by glucosamine chemical exchange saturation transfer MRI	0:83	Molecular imaging of cancer by glucosamine chemical exchange saturation transfer MRI: A preclinical study.
33103831	4	20	theme	exchangeable	705:716	arg1	protons					718:724	the hydroxyl, amine/amide exchangeable protons	679:724	protons	718:724	Significant CEST effects were observed at around 1.5, 3.6 and -3.4 ppm, corresponding to the hydroxyl, amine/amide exchangeable protons and for the Nuclear Overhauser Enhancement (NOE), respectively.
33103831	2	21	theme	different	389:397	arg1	regions					409:415	the different frequency regions	385:415	the different frequency regions of irradiation	385:430	Translation of the GlcN CEST method to the clinical application requires evaluation of its sensitivity to the different frequency regions of irradiation.
33103831	6	22	theme	uridine	1115:1121	arg1	substrate					1180:1188	a substrate	1178:1188	a substrate for the O-linked and N-linked glycosylated proteins that may be associated with the increase of the NOE signal	1178:1299	The CEST contribution can be attributed to several phosphorylated products of GlcN, including uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc), which is a substrate for the O-linked and N-linked glycosylated proteins that may be associated with the increase of the NOE signal.
33103831	6	22	theme	uridine	1115:1121	arg1	UDP-GlcNAc					1156:1165	UDP-GlcNAc	1156:1165	UDP-GlcNAc	1156:1165	The CEST contribution can be attributed to several phosphorylated products of GlcN, including uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc), which is a substrate for the O-linked and N-linked glycosylated proteins that may be associated with the increase of the NOE signal.
33103831	6	22	theme	uridine	1115:1121	arg1	diphosphate-N-acetylglucosamine					1123:1153	uridine diphosphate-N-acetylglucosamine	1115:1153	uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc)	1115:1166	The CEST contribution can be attributed to several phosphorylated products of GlcN, including uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc), which is a substrate for the O-linked and N-linked glycosylated proteins that may be associated with the increase of the NOE signal.
33103831	7	23	theme	GlcN	1458:1461	arg1	effect					1448:1453	the enhanced CEST effect	1430:1453	the enhanced CEST effect of GlcN	1430:1461	The observation of a significant amount of lactate among the metabolic products hints at acidification as one of the sources of the enhanced CEST effect of GlcN.
33103831	5	24	theme	tumor	963:967	arg1	model					969:973	the same tumor model	954:973	the same tumor model	954:973	The sources of the observed CEST effects were investigated by identifying the GlcN metabolic products as observed by 13 C NMR spectroscopy studies of extracts from the same tumor model following treatment with [UL-13 C] -GlcN·HCl.
33103831	8	25	theme	new	1496:1498	arg1	approach					1500:1507	a new approach	1494:1507	a new approach for clinical molecular imaging that enables the detection of metabolically active tumors and may play a role in other diseases	1494:1634	The proposed method may offer a new approach for clinical molecular imaging that enables the detection of metabolically active tumors and may play a role in other diseases.
33103831	3	26	theme	GlcN	455:458	arg1	signal					460:465	the GlcN signal	451:465	the GlcN signal	451:465	Hence, imaging of the GlcN signal was established for the full Z spectra recorded following GlcN administration to mice bearing implanted 4T1 breast tumors.
33103831	0	27	theme	chemical	43:50	arg1	MRI					81:83	glucosamine chemical exchange saturation transfer MRI	31:83	glucosamine chemical exchange saturation transfer MRI	31:83	Molecular imaging of cancer by glucosamine chemical exchange saturation transfer MRI: A preclinical study.
33103831	7	28	theme	effect	1448:1453	arg1	sources					1419:1425	the sources	1415:1425	the sources of the enhanced CEST effect of GlcN	1415:1461	The observation of a significant amount of lactate among the metabolic products hints at acidification as one of the sources of the enhanced CEST effect of GlcN.
33103831	6	29	theme	several	1064:1070	arg1	products					1087:1094	several phosphorylated products	1064:1094	several phosphorylated products	1064:1094	The CEST contribution can be attributed to several phosphorylated products of GlcN, including uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc), which is a substrate for the O-linked and N-linked glycosylated proteins that may be associated with the increase of the NOE signal.
33103831	6	29	theme	several	1064:1070	arg1	diphosphate-N-acetylglucosamine					1123:1153	uridine diphosphate-N-acetylglucosamine	1115:1153	uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc)	1115:1166	The CEST contribution can be attributed to several phosphorylated products of GlcN, including uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc), which is a substrate for the O-linked and N-linked glycosylated proteins that may be associated with the increase of the NOE signal.
33103831	4	30	theme	Overhauser	746:755	arg1	Enhancement					757:767	the Nuclear Overhauser Enhancement	734:767	the Nuclear Overhauser Enhancement (NOE)	734:773	Significant CEST effects were observed at around 1.5, 3.6 and -3.4 ppm, corresponding to the hydroxyl, amine/amide exchangeable protons and for the Nuclear Overhauser Enhancement (NOE), respectively.
33103831	4	30	theme	Overhauser	746:755	arg1	NOE					770:772	NOE	770:772	NOE	770:772	Significant CEST effects were observed at around 1.5, 3.6 and -3.4 ppm, corresponding to the hydroxyl, amine/amide exchangeable protons and for the Nuclear Overhauser Enhancement (NOE), respectively.
33103831	2	31	theme	GlcN	298:301	arg1	method					308:313	the GlcN CEST method	294:313	the GlcN CEST method	294:313	Translation of the GlcN CEST method to the clinical application requires evaluation of its sensitivity to the different frequency regions of irradiation.
33103831	8	32	theme	active	1584:1589	arg1	tumors					1591:1596	metabolically active tumors	1570:1596	metabolically active tumors	1570:1596	The proposed method may offer a new approach for clinical molecular imaging that enables the detection of metabolically active tumors and may play a role in other diseases.
33103831	1	33	with	agent	154:158	arg1	profile					185:191	an excellent safety profile	165:191	an excellent safety profile to detect cancer with the chemical exchange saturation transfer (CEST) MRI technique	165:276	Glucosamine (GlcN) was recently proposed as an agent with an excellent safety profile to detect cancer with the chemical exchange saturation transfer (CEST) MRI technique.
33103831	7	34	theme	enhanced	1434:1441	arg1	effect					1448:1453	the enhanced CEST effect	1430:1453	the enhanced CEST effect of GlcN	1430:1461	The observation of a significant amount of lactate among the metabolic products hints at acidification as one of the sources of the enhanced CEST effect of GlcN.
33103831	5	35	theme	extracts	940:947	arg1	studies					929:935	13 C NMR spectroscopy studies	907:935	13 C NMR spectroscopy studies of extracts from the same tumor model	907:973	The sources of the observed CEST effects were investigated by identifying the GlcN metabolic products as observed by 13 C NMR spectroscopy studies of extracts from the same tumor model following treatment with [UL-13 C] -GlcN·HCl.
33103831	8	36	theme	molecular	1522:1530	arg1	imaging					1532:1538	clinical molecular imaging	1513:1538	clinical molecular imaging that enables the detection of metabolically active tumors and may play a role in other diseases	1513:1634	The proposed method may offer a new approach for clinical molecular imaging that enables the detection of metabolically active tumors and may play a role in other diseases.
33103831	5	37	from	studies	929:935	arg1	model					969:973	the same tumor model	954:973	the same tumor model	954:973	The sources of the observed CEST effects were investigated by identifying the GlcN metabolic products as observed by 13 C NMR spectroscopy studies of extracts from the same tumor model following treatment with [UL-13 C] -GlcN·HCl.
33103831	1	38	theme	chemical	219:226	arg1	technique					268:276	the chemical exchange saturation transfer (CEST) MRI technique	215:276	the chemical exchange saturation transfer (CEST) MRI technique	215:276	Glucosamine (GlcN) was recently proposed as an agent with an excellent safety profile to detect cancer with the chemical exchange saturation transfer (CEST) MRI technique.
33103831	6	39	theme	CEST	1025:1028	arg1	contribution					1030:1041	The CEST contribution	1021:1041	The CEST contribution	1021:1041	The CEST contribution can be attributed to several phosphorylated products of GlcN, including uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc), which is a substrate for the O-linked and N-linked glycosylated proteins that may be associated with the increase of the NOE signal.
33103831	1	40	theme	saturation	237:246	arg1	technique					268:276	the chemical exchange saturation transfer (CEST) MRI technique	215:276	the chemical exchange saturation transfer (CEST) MRI technique	215:276	Glucosamine (GlcN) was recently proposed as an agent with an excellent safety profile to detect cancer with the chemical exchange saturation transfer (CEST) MRI technique.
33103831	2	41	theme	method	308:313	arg1	Translation					279:289	Translation	279:289	Translation of the GlcN CEST method to the clinical application	279:341	Translation of the GlcN CEST method to the clinical application requires evaluation of its sensitivity to the different frequency regions of irradiation.
33103831	6	42	theme	N-linked	1211:1218	arg1	proteins					1233:1240	the O-linked and N-linked glycosylated proteins	1194:1240	the O-linked and N-linked glycosylated proteins that may be associated with the increase of the NOE signal	1194:1299	The CEST contribution can be attributed to several phosphorylated products of GlcN, including uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc), which is a substrate for the O-linked and N-linked glycosylated proteins that may be associated with the increase of the NOE signal.
33103831	3	43	theme	full	491:494	arg1	spectra					498:504	the full Z spectra	487:504	the full Z spectra recorded following GlcN administration to mice bearing implanted 4T1 breast tumors	487:587	Hence, imaging of the GlcN signal was established for the full Z spectra recorded following GlcN administration to mice bearing implanted 4T1 breast tumors.
33103831	8	44	theme	other	1621:1625	arg1	diseases					1627:1634	other diseases	1621:1634	other diseases	1621:1634	The proposed method may offer a new approach for clinical molecular imaging that enables the detection of metabolically active tumors and may play a role in other diseases.
33103831	6	45	theme	O-linked	1198:1205	arg1	proteins					1233:1240	the O-linked and N-linked glycosylated proteins	1194:1240	the O-linked and N-linked glycosylated proteins that may be associated with the increase of the NOE signal	1194:1299	The CEST contribution can be attributed to several phosphorylated products of GlcN, including uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc), which is a substrate for the O-linked and N-linked glycosylated proteins that may be associated with the increase of the NOE signal.
33103831	5	46	theme	CEST	818:821	arg1	effects					823:829	the observed CEST effects	805:829	the observed CEST effects	805:829	The sources of the observed CEST effects were investigated by identifying the GlcN metabolic products as observed by 13 C NMR spectroscopy studies of extracts from the same tumor model following treatment with [UL-13 C] -GlcN·HCl.
33103831	6	47	theme	GlcN	1099:1102	arg1	products					1087:1094	several phosphorylated products	1064:1094	several phosphorylated products	1064:1094	The CEST contribution can be attributed to several phosphorylated products of GlcN, including uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc), which is a substrate for the O-linked and N-linked glycosylated proteins that may be associated with the increase of the NOE signal.
33103831	6	47	theme	GlcN	1099:1102	arg1	diphosphate-N-acetylglucosamine					1123:1153	uridine diphosphate-N-acetylglucosamine	1115:1153	uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc)	1115:1166	The CEST contribution can be attributed to several phosphorylated products of GlcN, including uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc), which is a substrate for the O-linked and N-linked glycosylated proteins that may be associated with the increase of the NOE signal.
33103831	6	48	link	O-linked	1198:1205	arg1	proteins					1233:1240	the O-linked and N-linked glycosylated proteins	1194:1240	the O-linked and N-linked glycosylated proteins that may be associated with the increase of the NOE signal	1194:1299	The CEST contribution can be attributed to several phosphorylated products of GlcN, including uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc), which is a substrate for the O-linked and N-linked glycosylated proteins that may be associated with the increase of the NOE signal.
33103831	6	49	theme	NOE	1290:1292	arg1	signal					1294:1299	the NOE signal	1286:1299	the NOE signal	1286:1299	The CEST contribution can be attributed to several phosphorylated products of GlcN, including uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc), which is a substrate for the O-linked and N-linked glycosylated proteins that may be associated with the increase of the NOE signal.
33103831	1	50	theme	excellent	168:176	arg1	profile					185:191	an excellent safety profile	165:191	an excellent safety profile to detect cancer with the chemical exchange saturation transfer (CEST) MRI technique	165:276	Glucosamine (GlcN) was recently proposed as an agent with an excellent safety profile to detect cancer with the chemical exchange saturation transfer (CEST) MRI technique.
33103831	7	51	theme	lactate	1345:1351	arg1	lactate					1345:1351	lactate	1345:1351	lactate	1345:1351	The observation of a significant amount of lactate among the metabolic products hints at acidification as one of the sources of the enhanced CEST effect of GlcN.
33103831	7	51	theme	lactate	1345:1351	arg1	amount					1335:1340	a significant amount	1321:1340	a significant amount of lactate	1321:1351	The observation of a significant amount of lactate among the metabolic products hints at acidification as one of the sources of the enhanced CEST effect of GlcN.
33103831	6	52	link	N-linked	1211:1218	arg1	proteins					1233:1240	the O-linked and N-linked glycosylated proteins	1194:1240	the O-linked and N-linked glycosylated proteins that may be associated with the increase of the NOE signal	1194:1299	The CEST contribution can be attributed to several phosphorylated products of GlcN, including uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc), which is a substrate for the O-linked and N-linked glycosylated proteins that may be associated with the increase of the NOE signal.
33103831	4	53	theme	CEST	602:605	arg1	effects					607:613	Significant CEST effects	590:613	Significant CEST effects	590:613	Significant CEST effects were observed at around 1.5, 3.6 and -3.4 ppm, corresponding to the hydroxyl, amine/amide exchangeable protons and for the Nuclear Overhauser Enhancement (NOE), respectively.
33103831	3	54	theme	signal	460:465	arg1	imaging					440:446	imaging	440:446	imaging of the GlcN signal	440:465	Hence, imaging of the GlcN signal was established for the full Z spectra recorded following GlcN administration to mice bearing implanted 4T1 breast tumors.
33103831	3	55	theme	implanted	561:569	arg1	tumors					582:587	implanted 4T1 breast tumors	561:587	implanted 4T1 breast tumors	561:587	Hence, imaging of the GlcN signal was established for the full Z spectra recorded following GlcN administration to mice bearing implanted 4T1 breast tumors.
33103831	7	56	theme	amount	1335:1340	arg1	observation					1306:1316	The observation	1302:1316	The observation of a significant amount of lactate among the metabolic products	1302:1380	The observation of a significant amount of lactate among the metabolic products hints at acidification as one of the sources of the enhanced CEST effect of GlcN.
33103831	5	57	theme	metabolic	873:881	arg1	products					883:890	the GlcN metabolic products	864:890	the GlcN metabolic products as observed by 13 C NMR spectroscopy studies of extracts from the same tumor model	864:973	The sources of the observed CEST effects were investigated by identifying the GlcN metabolic products as observed by 13 C NMR spectroscopy studies of extracts from the same tumor model following treatment with [UL-13 C] -GlcN·HCl.
33103831	0	58	theme	transfer	72:79	arg1	MRI					81:83	glucosamine chemical exchange saturation transfer MRI	31:83	glucosamine chemical exchange saturation transfer MRI	31:83	Molecular imaging of cancer by glucosamine chemical exchange saturation transfer MRI: A preclinical study.
33103831	5	59	theme	C	1007:1007	arg1	-GlcN·HCl					1010:1018	[UL-13 C] -GlcN·HCl	1000:1018	[UL-13 C] -GlcN·HCl	1000:1018	The sources of the observed CEST effects were investigated by identifying the GlcN metabolic products as observed by 13 C NMR spectroscopy studies of extracts from the same tumor model following treatment with [UL-13 C] -GlcN·HCl.
33103831	3	60	theme	breast	575:580	arg1	tumors					582:587	implanted 4T1 breast tumors	561:587	implanted 4T1 breast tumors	561:587	Hence, imaging of the GlcN signal was established for the full Z spectra recorded following GlcN administration to mice bearing implanted 4T1 breast tumors.
33103831	6	61	gly	glycosylated	1220:1231	arg1	proteins					1233:1240	the O-linked and N-linked glycosylated proteins	1194:1240	the O-linked and N-linked glycosylated proteins that may be associated with the increase of the NOE signal	1194:1299	The CEST contribution can be attributed to several phosphorylated products of GlcN, including uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc), which is a substrate for the O-linked and N-linked glycosylated proteins that may be associated with the increase of the NOE signal.
33103831	0	62	theme	preclinical	88:98	arg1	study					100:104	A preclinical study	86:104	A preclinical study	86:104	Molecular imaging of cancer by glucosamine chemical exchange saturation transfer MRI: A preclinical study.
33103831	5	63	theme	C	910:910	arg1	studies					929:935	13 C NMR spectroscopy studies	907:935	13 C NMR spectroscopy studies of extracts from the same tumor model	907:973	The sources of the observed CEST effects were investigated by identifying the GlcN metabolic products as observed by 13 C NMR spectroscopy studies of extracts from the same tumor model following treatment with [UL-13 C] -GlcN·HCl.
33103831	0	64	dep	study	100:104	arg1	imaging					10:16	Molecular imaging	0:16	Molecular imaging of cancer by glucosamine chemical exchange saturation transfer MRI	0:83	Molecular imaging of cancer by glucosamine chemical exchange saturation transfer MRI: A preclinical study.
33103831	5	65	dep	products	883:890	arg1	observed					895:902	observed	895:902	observed by 13 C NMR spectroscopy studies of extracts from the same tumor model	895:973	The sources of the observed CEST effects were investigated by identifying the GlcN metabolic products as observed by 13 C NMR spectroscopy studies of extracts from the same tumor model following treatment with [UL-13 C] -GlcN·HCl.
33103831	5	66	theme	spectroscopy	916:927	arg1	studies					929:935	13 C NMR spectroscopy studies	907:935	13 C NMR spectroscopy studies of extracts from the same tumor model	907:973	The sources of the observed CEST effects were investigated by identifying the GlcN metabolic products as observed by 13 C NMR spectroscopy studies of extracts from the same tumor model following treatment with [UL-13 C] -GlcN·HCl.
33103831	8	67	theme	proposed	1468:1475	arg1	method					1477:1482	The proposed method	1464:1482	The proposed method	1464:1482	The proposed method may offer a new approach for clinical molecular imaging that enables the detection of metabolically active tumors and may play a role in other diseases.
33103831	5	68	with	treatment	985:993	arg1	-GlcN·HCl					1010:1018	[UL-13 C] -GlcN·HCl	1000:1018	[UL-13 C] -GlcN·HCl	1000:1018	The sources of the observed CEST effects were investigated by identifying the GlcN metabolic products as observed by 13 C NMR spectroscopy studies of extracts from the same tumor model following treatment with [UL-13 C] -GlcN·HCl.
33103831	4	69	theme	hydroxyl	683:690	arg1	protons					718:724	the hydroxyl, amine/amide exchangeable protons	679:724	protons	718:724	Significant CEST effects were observed at around 1.5, 3.6 and -3.4 ppm, corresponding to the hydroxyl, amine/amide exchangeable protons and for the Nuclear Overhauser Enhancement (NOE), respectively.
33103831	0	70	theme	cancer	21:26	arg1	imaging					10:16	Molecular imaging	0:16	Molecular imaging of cancer by glucosamine chemical exchange saturation transfer MRI	0:83	Molecular imaging of cancer by glucosamine chemical exchange saturation transfer MRI: A preclinical study.
33103831	1	71	theme	CEST	258:261	arg1	technique					268:276	the chemical exchange saturation transfer (CEST) MRI technique	215:276	the chemical exchange saturation transfer (CEST) MRI technique	215:276	Glucosamine (GlcN) was recently proposed as an agent with an excellent safety profile to detect cancer with the chemical exchange saturation transfer (CEST) MRI technique.
33103831	2	72	theme	frequency	399:407	arg1	regions					409:415	the different frequency regions	385:415	the different frequency regions of irradiation	385:430	Translation of the GlcN CEST method to the clinical application requires evaluation of its sensitivity to the different frequency regions of irradiation.
33103831	4	73	theme	amine/amide	693:703	arg1	protons					718:724	the hydroxyl, amine/amide exchangeable protons	679:724	protons	718:724	Significant CEST effects were observed at around 1.5, 3.6 and -3.4 ppm, corresponding to the hydroxyl, amine/amide exchangeable protons and for the Nuclear Overhauser Enhancement (NOE), respectively.
33103831	0	74	theme	glucosamine	31:41	arg1	MRI					81:83	glucosamine chemical exchange saturation transfer MRI	31:83	glucosamine chemical exchange saturation transfer MRI	31:83	Molecular imaging of cancer by glucosamine chemical exchange saturation transfer MRI: A preclinical study.
33103831	1	75	theme	MRI	264:266	arg1	technique					268:276	the chemical exchange saturation transfer (CEST) MRI technique	215:276	the chemical exchange saturation transfer (CEST) MRI technique	215:276	Glucosamine (GlcN) was recently proposed as an agent with an excellent safety profile to detect cancer with the chemical exchange saturation transfer (CEST) MRI technique.
33103831	5	76	theme	same	958:961	arg1	model					969:973	the same tumor model	954:973	the same tumor model	954:973	The sources of the observed CEST effects were investigated by identifying the GlcN metabolic products as observed by 13 C NMR spectroscopy studies of extracts from the same tumor model following treatment with [UL-13 C] -GlcN·HCl.
33103831	2	77	theme	sensitivity	370:380	arg1	evaluation					352:361	evaluation	352:361	evaluation of its sensitivity to the different frequency regions of irradiation	352:430	Translation of the GlcN CEST method to the clinical application requires evaluation of its sensitivity to the different frequency regions of irradiation.
33103831	0	78	theme	exchange	52:59	arg1	MRI					81:83	glucosamine chemical exchange saturation transfer MRI	31:83	glucosamine chemical exchange saturation transfer MRI	31:83	Molecular imaging of cancer by glucosamine chemical exchange saturation transfer MRI: A preclinical study.
33103831	6	79	theme	phosphorylated	1072:1085	arg1	products					1087:1094	several phosphorylated products	1064:1094	several phosphorylated products	1064:1094	The CEST contribution can be attributed to several phosphorylated products of GlcN, including uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc), which is a substrate for the O-linked and N-linked glycosylated proteins that may be associated with the increase of the NOE signal.
33103831	6	79	theme	phosphorylated	1072:1085	arg1	diphosphate-N-acetylglucosamine					1123:1153	uridine diphosphate-N-acetylglucosamine	1115:1153	uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc)	1115:1166	The CEST contribution can be attributed to several phosphorylated products of GlcN, including uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc), which is a substrate for the O-linked and N-linked glycosylated proteins that may be associated with the increase of the NOE signal.
33103831	8	80	theme	clinical	1513:1520	arg1	imaging					1532:1538	clinical molecular imaging	1513:1538	clinical molecular imaging that enables the detection of metabolically active tumors and may play a role in other diseases	1513:1634	The proposed method may offer a new approach for clinical molecular imaging that enables the detection of metabolically active tumors and may play a role in other diseases.
33103831	2	81	theme	CEST	303:306	arg1	method					308:313	the GlcN CEST method	294:313	the GlcN CEST method	294:313	Translation of the GlcN CEST method to the clinical application requires evaluation of its sensitivity to the different frequency regions of irradiation.
33103831	8	82	theme	tumors	1591:1596	arg1	detection					1557:1565	the detection	1553:1565	the detection of metabolically active tumors	1553:1596	The proposed method may offer a new approach for clinical molecular imaging that enables the detection of metabolically active tumors and may play a role in other diseases.
33103831	4	83	theme	Nuclear	738:744	arg1	Enhancement					757:767	the Nuclear Overhauser Enhancement	734:767	the Nuclear Overhauser Enhancement (NOE)	734:773	Significant CEST effects were observed at around 1.5, 3.6 and -3.4 ppm, corresponding to the hydroxyl, amine/amide exchangeable protons and for the Nuclear Overhauser Enhancement (NOE), respectively.
33103831	4	83	theme	Nuclear	738:744	arg1	NOE					770:772	NOE	770:772	NOE	770:772	Significant CEST effects were observed at around 1.5, 3.6 and -3.4 ppm, corresponding to the hydroxyl, amine/amide exchangeable protons and for the Nuclear Overhauser Enhancement (NOE), respectively.
33103831	7	84	theme	CEST	1443:1446	arg1	effect					1448:1453	the enhanced CEST effect	1430:1453	the enhanced CEST effect of GlcN	1430:1461	The observation of a significant amount of lactate among the metabolic products hints at acidification as one of the sources of the enhanced CEST effect of GlcN.
33103831	4	85	theme	Significant	590:600	arg1	effects					607:613	Significant CEST effects	590:613	Significant CEST effects	590:613	Significant CEST effects were observed at around 1.5, 3.6 and -3.4 ppm, corresponding to the hydroxyl, amine/amide exchangeable protons and for the Nuclear Overhauser Enhancement (NOE), respectively.
33103831	2	86	theme	clinical	322:329	arg1	application					331:341	the clinical application	318:341	the clinical application	318:341	Translation of the GlcN CEST method to the clinical application requires evaluation of its sensitivity to the different frequency regions of irradiation.
34013301	0	0	theme	Spike	98:102	arg1	protein					104:110	the SARS-CoV-2 Spike protein	83:110	the SARS-CoV-2 Spike protein	83:110	Plasmodium infection induces cross-reactive antibodies to carbohydrate epitopes on the SARS-CoV-2 Spike protein.
34013301	3	1	theme	protein-based	534:546	arg1	assays					548:553	Spike protein-based assays	528:553	Spike protein-based assays	528:553	Sero-surveillance is critical for monitoring and projecting disease burden and risk during the pandemic; however, routine use of Spike protein-based assays may overestimate SARS-CoV-2 exposure and population-level immunity in malaria-endemic countries.
34013301	0	2	theme	SARS-CoV-2	87:96	arg1	protein					104:110	the SARS-CoV-2 Spike protein	83:110	the SARS-CoV-2 Spike protein	83:110	Plasmodium infection induces cross-reactive antibodies to carbohydrate epitopes on the SARS-CoV-2 Spike protein.
34013301	1	3	theme	antibodies	179:188	arg1	levels					169:174	high levels	164:174	high levels of antibodies that cross-react with the SARS-CoV-2 Spike protein	164:239	Individuals with acute malaria infection generated high levels of antibodies that cross-react with the SARS-CoV-2 Spike protein.
34013301	0	4	from	epitopes	71:78	arg1	protein					104:110	the SARS-CoV-2 Spike protein	83:110	the SARS-CoV-2 Spike protein	83:110	Plasmodium infection induces cross-reactive antibodies to carbohydrate epitopes on the SARS-CoV-2 Spike protein.
34013301	3	5	theme	population-level	596:611	arg1	immunity					613:620	population-level immunity	596:620	population-level immunity	596:620	Sero-surveillance is critical for monitoring and projecting disease burden and risk during the pandemic; however, routine use of Spike protein-based assays may overestimate SARS-CoV-2 exposure and population-level immunity in malaria-endemic countries.
34013301	2	6	theme	in	378:379	arg1	SARS-CoV-2					387:396	in vitro SARS-CoV-2	378:396	in vitro SARS-CoV-2	378:396	Cross-reactive antibodies specifically recognized the sialic acid moiety on N-linked glycans of the Spike protein and do not neutralize in vitro SARS-CoV-2.
34013301	2	7	theme	N-linked	318:325	arg1	glycans					327:333	N-linked glycans	318:333	N-linked glycans of the Spike protein	318:354	Cross-reactive antibodies specifically recognized the sialic acid moiety on N-linked glycans of the Spike protein and do not neutralize in vitro SARS-CoV-2.
34013301	3	8	theme	projecting	448:457	arg1	burden					467:472	projecting disease burden	448:472	projecting disease burden	448:472	Sero-surveillance is critical for monitoring and projecting disease burden and risk during the pandemic; however, routine use of Spike protein-based assays may overestimate SARS-CoV-2 exposure and population-level immunity in malaria-endemic countries.
34013301	1	9	with	Individuals	113:123	arg1	infection					144:152	acute malaria infection	130:152	acute malaria infection	130:152	Individuals with acute malaria infection generated high levels of antibodies that cross-react with the SARS-CoV-2 Spike protein.
34013301	3	10	theme	malaria-endemic	625:639	arg1	countries					641:649	malaria-endemic countries	625:649	malaria-endemic countries	625:649	Sero-surveillance is critical for monitoring and projecting disease burden and risk during the pandemic; however, routine use of Spike protein-based assays may overestimate SARS-CoV-2 exposure and population-level immunity in malaria-endemic countries.
34013301	0	11	theme	Plasmodium	0:9	arg1	infection					11:19	Plasmodium infection	0:19	Plasmodium infection	0:19	Plasmodium infection induces cross-reactive antibodies to carbohydrate epitopes on the SARS-CoV-2 Spike protein.
34013301	2	12	link	N-linked	318:325	arg1	glycans					327:333	N-linked glycans	318:333	N-linked glycans of the Spike protein	318:354	Cross-reactive antibodies specifically recognized the sialic acid moiety on N-linked glycans of the Spike protein and do not neutralize in vitro SARS-CoV-2.
34013301	2	13	theme	protein	348:354	arg1	glycans					327:333	N-linked glycans	318:333	N-linked glycans of the Spike protein	318:354	Cross-reactive antibodies specifically recognized the sialic acid moiety on N-linked glycans of the Spike protein and do not neutralize in vitro SARS-CoV-2.
34013301	0	14	theme	cross-reactive	29:42	arg1	antibodies					44:53	cross-reactive antibodies	29:53	cross-reactive antibodies to carbohydrate epitopes on the SARS-CoV-2 Spike protein	29:110	Plasmodium infection induces cross-reactive antibodies to carbohydrate epitopes on the SARS-CoV-2 Spike protein.
34013301	2	15	dep	in	378:379	arg1	vitro					381:385	vitro	381:385	vitro	381:385	Cross-reactive antibodies specifically recognized the sialic acid moiety on N-linked glycans of the Spike protein and do not neutralize in vitro SARS-CoV-2.
34013301	1	16	theme	SARS-CoV-2	216:225	arg1	protein					233:239	the SARS-CoV-2 Spike protein	212:239	the SARS-CoV-2 Spike protein	212:239	Individuals with acute malaria infection generated high levels of antibodies that cross-react with the SARS-CoV-2 Spike protein.
34013301	3	17	theme	SARS-CoV-2	572:581	arg1	exposure					583:590	SARS-CoV-2 exposure	572:590	SARS-CoV-2 exposure	572:590	Sero-surveillance is critical for monitoring and projecting disease burden and risk during the pandemic; however, routine use of Spike protein-based assays may overestimate SARS-CoV-2 exposure and population-level immunity in malaria-endemic countries.
34013301	3	18	theme	assays	548:553	arg1	use					521:523	routine use	513:523	routine use of Spike protein-based assays	513:553	Sero-surveillance is critical for monitoring and projecting disease burden and risk during the pandemic; however, routine use of Spike protein-based assays may overestimate SARS-CoV-2 exposure and population-level immunity in malaria-endemic countries.
34013301	1	19	theme	Spike	227:231	arg1	protein					233:239	the SARS-CoV-2 Spike protein	212:239	the SARS-CoV-2 Spike protein	212:239	Individuals with acute malaria infection generated high levels of antibodies that cross-react with the SARS-CoV-2 Spike protein.
34013301	3	20	theme	disease	459:465	arg1	burden					467:472	projecting disease burden	448:472	projecting disease burden	448:472	Sero-surveillance is critical for monitoring and projecting disease burden and risk during the pandemic; however, routine use of Spike protein-based assays may overestimate SARS-CoV-2 exposure and population-level immunity in malaria-endemic countries.
34013301	3	21	theme	Spike	528:532	arg1	assays					548:553	Spike protein-based assays	528:553	Spike protein-based assays	528:553	Sero-surveillance is critical for monitoring and projecting disease burden and risk during the pandemic; however, routine use of Spike protein-based assays may overestimate SARS-CoV-2 exposure and population-level immunity in malaria-endemic countries.
34013301	1	22	theme	acute	130:134	arg1	infection					144:152	acute malaria infection	130:152	acute malaria infection	130:152	Individuals with acute malaria infection generated high levels of antibodies that cross-react with the SARS-CoV-2 Spike protein.
34013301	0	23	theme	carbohydrate	58:69	arg1	epitopes					71:78	carbohydrate epitopes	58:78	carbohydrate epitopes on the SARS-CoV-2 Spike protein	58:110	Plasmodium infection induces cross-reactive antibodies to carbohydrate epitopes on the SARS-CoV-2 Spike protein.
34013301	2	24	theme	Cross-reactive	242:255	arg1	antibodies					257:266	Cross-reactive antibodies	242:266	Cross-reactive antibodies	242:266	Cross-reactive antibodies specifically recognized the sialic acid moiety on N-linked glycans of the Spike protein and do not neutralize in vitro SARS-CoV-2.
34013301	1	25	theme	malaria	136:142	arg1	infection					144:152	acute malaria infection	130:152	acute malaria infection	130:152	Individuals with acute malaria infection generated high levels of antibodies that cross-react with the SARS-CoV-2 Spike protein.
34013301	3	26	theme	routine	513:519	arg1	use					521:523	routine use	513:523	routine use of Spike protein-based assays	513:553	Sero-surveillance is critical for monitoring and projecting disease burden and risk during the pandemic; however, routine use of Spike protein-based assays may overestimate SARS-CoV-2 exposure and population-level immunity in malaria-endemic countries.
34013301	2	27	theme	Spike	342:346	arg1	protein					348:354	the Spike protein	338:354	the Spike protein	338:354	Cross-reactive antibodies specifically recognized the sialic acid moiety on N-linked glycans of the Spike protein and do not neutralize in vitro SARS-CoV-2.
34013301	2	28	theme	acid	303:306	arg1	moiety					308:313	the sialic acid moiety	292:313	the sialic acid moiety	292:313	Cross-reactive antibodies specifically recognized the sialic acid moiety on N-linked glycans of the Spike protein and do not neutralize in vitro SARS-CoV-2.
34013301	2	29	theme	sialic	296:301	arg1	moiety					308:313	the sialic acid moiety	292:313	the sialic acid moiety	292:313	Cross-reactive antibodies specifically recognized the sialic acid moiety on N-linked glycans of the Spike protein and do not neutralize in vitro SARS-CoV-2.
34013301	1	30	theme	high	164:167	arg1	levels					169:174	high levels	164:174	high levels of antibodies that cross-react with the SARS-CoV-2 Spike protein	164:239	Individuals with acute malaria infection generated high levels of antibodies that cross-react with the SARS-CoV-2 Spike protein.
34942961	0	0	theme	Complex	63:69	arg1	Pectin-Polyphenols					71:88	Complex Pectin-Polyphenols	63:88	Complex Pectin-Polyphenols	63:88	Effect of the Olive Oil Extraction Process on the Formation of Complex Pectin-Polyphenols and Their Antioxidant and Antiproliferative Activities.
34942961	5	1	from	content	987:993	arg1	extracts					1001:1008	AB extracts	998:1008	AB extracts	998:1008	The polyphenols associated mainly with the acidic pectin substance, with a higher content in AB extracts, seem to be responsible for these activities, and appear to maintain their activities in part after complexation.
34942961	6	2	theme	pectic	1217:1222	arg1	polysaccharides					1224:1238	pectic polysaccharides	1217:1238	pectic polysaccharides	1217:1238	However, even in olive fruit extracts with smaller amounts of phenols in their compositions, pectic polysaccharides may also be involved in antioxidant and antiproliferative activities.
34942961	6	3	from	amounts	1175:1181	arg1	compositions					1203:1214	their compositions	1197:1214	their compositions	1197:1214	However, even in olive fruit extracts with smaller amounts of phenols in their compositions, pectic polysaccharides may also be involved in antioxidant and antiproliferative activities.
34942961	6	4	from	phenols	1186:1192	arg1	compositions					1203:1214	their compositions	1197:1214	their compositions	1197:1214	However, even in olive fruit extracts with smaller amounts of phenols in their compositions, pectic polysaccharides may also be involved in antioxidant and antiproliferative activities.
34942961	2	5	theme	fruits	560:565	arg1	fruits					560:565	olive fruits	554:565	olive fruits	554:565	For this, pectin was extracted from fresh olive fruits and compared to the pectin isolated from the paste resulting from the extraction of the olive oil after milling with malaxation at 30 °C/30 min and subsequent centrifugation of the olive paste from the same lot of olive fruits in a system called ABENCOR (AB).
34942961	2	5	theme	fruits	560:565	arg1	lot					547:549	the same lot	538:549	the same lot of olive fruits in a system called ABENCOR (AB)	538:597	For this, pectin was extracted from fresh olive fruits and compared to the pectin isolated from the paste resulting from the extraction of the olive oil after milling with malaxation at 30 °C/30 min and subsequent centrifugation of the olive paste from the same lot of olive fruits in a system called ABENCOR (AB).
34942961	1	6	theme	olive	255:259	arg1	process					276:282	the olive oil extraction process	251:282	the olive oil extraction process	251:282	The aim of this research was to investigate the interaction of phenols and pectic polysaccharides during the olive oil extraction process.
34942961	0	7	from	Effect	0:5	arg1	Activities					134:143	Their Antioxidant and Antiproliferative Activities	94:143	Their Antioxidant and Antiproliferative Activities	94:143	Effect of the Olive Oil Extraction Process on the Formation of Complex Pectin-Polyphenols and Their Antioxidant and Antiproliferative Activities.
34942961	0	7	from	Effect	0:5	arg1	Formation					50:58	the Formation	46:58	the Formation of Complex Pectin-Polyphenols	46:88	Effect of the Olive Oil Extraction Process on the Formation of Complex Pectin-Polyphenols and Their Antioxidant and Antiproliferative Activities.
34942961	2	8	theme	olive	554:558	arg1	fruits					560:565	olive fruits	554:565	olive fruits	554:565	For this, pectin was extracted from fresh olive fruits and compared to the pectin isolated from the paste resulting from the extraction of the olive oil after milling with malaxation at 30 °C/30 min and subsequent centrifugation of the olive paste from the same lot of olive fruits in a system called ABENCOR (AB).
34942961	5	9	theme	acidic	948:953	arg1	substance					962:970	the acidic pectin substance	944:970	the acidic pectin substance	944:970	The polyphenols associated mainly with the acidic pectin substance, with a higher content in AB extracts, seem to be responsible for these activities, and appear to maintain their activities in part after complexation.
34942961	1	10	theme	oil	261:263	arg1	process					276:282	the olive oil extraction process	251:282	the olive oil extraction process	251:282	The aim of this research was to investigate the interaction of phenols and pectic polysaccharides during the olive oil extraction process.
34942961	0	11	theme	Pectin-Polyphenols	71:88	arg1	Activities					134:143	Their Antioxidant and Antiproliferative Activities	94:143	Their Antioxidant and Antiproliferative Activities	94:143	Effect of the Olive Oil Extraction Process on the Formation of Complex Pectin-Polyphenols and Their Antioxidant and Antiproliferative Activities.
34942961	0	11	theme	Pectin-Polyphenols	71:88	arg1	Formation					50:58	the Formation	46:58	the Formation of Complex Pectin-Polyphenols	46:88	Effect of the Olive Oil Extraction Process on the Formation of Complex Pectin-Polyphenols and Their Antioxidant and Antiproliferative Activities.
34942961	3	12	theme	olive	670:674	arg1	process					691:697	the olive oil extraction process	666:697	the olive oil extraction process	666:697	The results indicate that these interactions were enhanced during the olive oil extraction process.
34942961	1	13	theme	extraction	265:274	arg1	process					276:282	the olive oil extraction process	251:282	the olive oil extraction process	251:282	The aim of this research was to investigate the interaction of phenols and pectic polysaccharides during the olive oil extraction process.
34942961	0	14	theme	Antioxidant	100:110	arg1	Activities					134:143	Their Antioxidant and Antiproliferative Activities	94:143	Their Antioxidant and Antiproliferative Activities	94:143	Effect of the Olive Oil Extraction Process on the Formation of Complex Pectin-Polyphenols and Their Antioxidant and Antiproliferative Activities.
34942961	3	15	theme	oil	676:678	arg1	process					691:697	the olive oil extraction process	666:697	the olive oil extraction process	666:697	The results indicate that these interactions were enhanced during the olive oil extraction process.
34942961	6	16	theme	phenols	1186:1192	arg1	phenols					1186:1192	phenols	1186:1192	phenols in their compositions	1186:1214	However, even in olive fruit extracts with smaller amounts of phenols in their compositions, pectic polysaccharides may also be involved in antioxidant and antiproliferative activities.
34942961	6	16	theme	phenols	1186:1192	arg1	amounts					1175:1181	smaller amounts	1167:1181	smaller amounts of phenols in their compositions	1167:1214	However, even in olive fruit extracts with smaller amounts of phenols in their compositions, pectic polysaccharides may also be involved in antioxidant and antiproliferative activities.
34942961	4	17	theme	resulting	717:725	arg1	extracts					730:737	the resulting AB extracts	713:737	the resulting AB extracts	713:737	In addition, the resulting AB extracts exhibited high antioxidant activity (ORAC) and strong antiproliferative activity in vitro against colon carcinoma Caco-2 cell lines compared to olive fruit extracts.
34942961	1	18	theme	research	162:169	arg1	aim					150:152	The aim	146:152	The aim of this research	146:169	The aim of this research was to investigate the interaction of phenols and pectic polysaccharides during the olive oil extraction process.
34942961	2	19	theme	oil	434:436	arg1	extraction					410:419	the extraction	406:419	the extraction of the olive oil after milling with malaxation at 30 °C/30 min and subsequent centrifugation of the olive paste from the same lot of olive fruits in a system called ABENCOR (AB)	406:597	For this, pectin was extracted from fresh olive fruits and compared to the pectin isolated from the paste resulting from the extraction of the olive oil after milling with malaxation at 30 °C/30 min and subsequent centrifugation of the olive paste from the same lot of olive fruits in a system called ABENCOR (AB).
34942961	6	20	theme	antiproliferative	1280:1296	arg1	activities					1298:1307	antioxidant and antiproliferative activities	1264:1307	antioxidant and antiproliferative activities	1264:1307	However, even in olive fruit extracts with smaller amounts of phenols in their compositions, pectic polysaccharides may also be involved in antioxidant and antiproliferative activities.
34942961	2	21	theme	olive	428:432	arg1	oil					434:436	the olive oil	424:436	the olive oil after milling with malaxation at 30 °C/30 min and subsequent centrifugation of the olive paste from the same lot of olive fruits in a system called ABENCOR (AB)	424:597	For this, pectin was extracted from fresh olive fruits and compared to the pectin isolated from the paste resulting from the extraction of the olive oil after milling with malaxation at 30 °C/30 min and subsequent centrifugation of the olive paste from the same lot of olive fruits in a system called ABENCOR (AB).
34942961	4	22	theme	antiproliferative	793:809	arg1	activity					811:818	strong antiproliferative activity	786:818	strong antiproliferative activity	786:818	In addition, the resulting AB extracts exhibited high antioxidant activity (ORAC) and strong antiproliferative activity in vitro against colon carcinoma Caco-2 cell lines compared to olive fruit extracts.
34942961	2	23	theme	same	542:545	arg1	fruits					560:565	olive fruits	554:565	olive fruits	554:565	For this, pectin was extracted from fresh olive fruits and compared to the pectin isolated from the paste resulting from the extraction of the olive oil after milling with malaxation at 30 °C/30 min and subsequent centrifugation of the olive paste from the same lot of olive fruits in a system called ABENCOR (AB).
34942961	2	23	theme	same	542:545	arg1	lot					547:549	the same lot	538:549	the same lot of olive fruits in a system called ABENCOR (AB)	538:597	For this, pectin was extracted from fresh olive fruits and compared to the pectin isolated from the paste resulting from the extraction of the olive oil after milling with malaxation at 30 °C/30 min and subsequent centrifugation of the olive paste from the same lot of olive fruits in a system called ABENCOR (AB).
34942961	6	24	theme	antioxidant	1264:1274	arg1	activities					1298:1307	antioxidant and antiproliferative activities	1264:1307	antioxidant and antiproliferative activities	1264:1307	However, even in olive fruit extracts with smaller amounts of phenols in their compositions, pectic polysaccharides may also be involved in antioxidant and antiproliferative activities.
34942961	4	25	theme	strong	786:791	arg1	activity					811:818	strong antiproliferative activity	786:818	strong antiproliferative activity	786:818	In addition, the resulting AB extracts exhibited high antioxidant activity (ORAC) and strong antiproliferative activity in vitro against colon carcinoma Caco-2 cell lines compared to olive fruit extracts.
34942961	0	26	theme	Olive	14:18	arg1	Process					35:41	the Olive Oil Extraction Process	10:41	the Olive Oil Extraction Process	10:41	Effect of the Olive Oil Extraction Process on the Formation of Complex Pectin-Polyphenols and Their Antioxidant and Antiproliferative Activities.
34942961	5	27	theme	pectin	955:960	arg1	substance					962:970	the acidic pectin substance	944:970	the acidic pectin substance	944:970	The polyphenols associated mainly with the acidic pectin substance, with a higher content in AB extracts, seem to be responsible for these activities, and appear to maintain their activities in part after complexation.
34942961	0	28	theme	Antiproliferative	116:132	arg1	Activities					134:143	Their Antioxidant and Antiproliferative Activities	94:143	Their Antioxidant and Antiproliferative Activities	94:143	Effect of the Olive Oil Extraction Process on the Formation of Complex Pectin-Polyphenols and Their Antioxidant and Antiproliferative Activities.
34942961	6	29	theme	olive	1141:1145	arg1	extracts					1153:1160	olive fruit extracts	1141:1160	olive fruit extracts with smaller amounts of phenols in their compositions	1141:1214	However, even in olive fruit extracts with smaller amounts of phenols in their compositions, pectic polysaccharides may also be involved in antioxidant and antiproliferative activities.
34942961	3	30	theme	extraction	680:689	arg1	process					691:697	the olive oil extraction process	666:697	the olive oil extraction process	666:697	The results indicate that these interactions were enhanced during the olive oil extraction process.
34942961	2	31	theme	olive	521:525	arg1	paste					527:531	the olive paste	517:531	the olive paste from the same lot of olive fruits in a system called ABENCOR (AB)	517:597	For this, pectin was extracted from fresh olive fruits and compared to the pectin isolated from the paste resulting from the extraction of the olive oil after milling with malaxation at 30 °C/30 min and subsequent centrifugation of the olive paste from the same lot of olive fruits in a system called ABENCOR (AB).
34942961	0	32	theme	Extraction	24:33	arg1	Process					35:41	the Olive Oil Extraction Process	10:41	the Olive Oil Extraction Process	10:41	Effect of the Olive Oil Extraction Process on the Formation of Complex Pectin-Polyphenols and Their Antioxidant and Antiproliferative Activities.
34942961	4	33	theme	olive	883:887	arg1	extracts					895:902	olive fruit extracts	883:902	olive fruit extracts	883:902	In addition, the resulting AB extracts exhibited high antioxidant activity (ORAC) and strong antiproliferative activity in vitro against colon carcinoma Caco-2 cell lines compared to olive fruit extracts.
34942961	6	34	with	extracts	1153:1160	arg1	phenols					1186:1192	phenols	1186:1192	phenols in their compositions	1186:1214	However, even in olive fruit extracts with smaller amounts of phenols in their compositions, pectic polysaccharides may also be involved in antioxidant and antiproliferative activities.
34942961	6	34	with	extracts	1153:1160	arg1	amounts					1175:1181	smaller amounts	1167:1181	smaller amounts of phenols in their compositions	1167:1214	However, even in olive fruit extracts with smaller amounts of phenols in their compositions, pectic polysaccharides may also be involved in antioxidant and antiproliferative activities.
34942961	0	35	theme	Oil	20:22	arg1	Process					35:41	the Olive Oil Extraction Process	10:41	the Olive Oil Extraction Process	10:41	Effect of the Olive Oil Extraction Process on the Formation of Complex Pectin-Polyphenols and Their Antioxidant and Antiproliferative Activities.
34942961	4	36	theme	antioxidant	754:764	arg1	activity					766:773	high antioxidant activity	749:773	high antioxidant activity (ORAC)	749:780	In addition, the resulting AB extracts exhibited high antioxidant activity (ORAC) and strong antiproliferative activity in vitro against colon carcinoma Caco-2 cell lines compared to olive fruit extracts.
34942961	4	36	theme	antioxidant	754:764	arg1	ORAC					776:779	ORAC	776:779	ORAC	776:779	In addition, the resulting AB extracts exhibited high antioxidant activity (ORAC) and strong antiproliferative activity in vitro against colon carcinoma Caco-2 cell lines compared to olive fruit extracts.
34942961	1	37	theme	phenols	209:215	arg1	interaction					194:204	the interaction	190:204	the interaction of phenols and pectic polysaccharides	190:242	The aim of this research was to investigate the interaction of phenols and pectic polysaccharides during the olive oil extraction process.
34942961	5	38	with	polyphenols	909:919	arg1	content					987:993	a higher content	978:993	a higher content in AB extracts	978:1008	The polyphenols associated mainly with the acidic pectin substance, with a higher content in AB extracts, seem to be responsible for these activities, and appear to maintain their activities in part after complexation.
34942961	4	39	theme	carcinoma	843:851	arg1	lines					865:869	colon carcinoma Caco-2 cell lines	837:869	colon carcinoma Caco-2 cell lines	837:869	In addition, the resulting AB extracts exhibited high antioxidant activity (ORAC) and strong antiproliferative activity in vitro against colon carcinoma Caco-2 cell lines compared to olive fruit extracts.
34942961	6	40	theme	fruit	1147:1151	arg1	extracts					1153:1160	olive fruit extracts	1141:1160	olive fruit extracts with smaller amounts of phenols in their compositions	1141:1214	However, even in olive fruit extracts with smaller amounts of phenols in their compositions, pectic polysaccharides may also be involved in antioxidant and antiproliferative activities.
34942961	0	41	theme	Process	35:41	arg1	Effect					0:5	Effect	0:5	Effect of the Olive Oil Extraction Process on the Formation of Complex Pectin-Polyphenols and Their Antioxidant and Antiproliferative Activities.	0:144	Effect of the Olive Oil Extraction Process on the Formation of Complex Pectin-Polyphenols and Their Antioxidant and Antiproliferative Activities.
34942961	2	42	theme	paste	527:531	arg1	min					480:482	30 °C/30 min	471:482	30 °C/30 min	471:482	For this, pectin was extracted from fresh olive fruits and compared to the pectin isolated from the paste resulting from the extraction of the olive oil after milling with malaxation at 30 °C/30 min and subsequent centrifugation of the olive paste from the same lot of olive fruits in a system called ABENCOR (AB).
34942961	2	42	theme	paste	527:531	arg1	centrifugation					499:512	subsequent centrifugation	488:512	subsequent centrifugation	488:512	For this, pectin was extracted from fresh olive fruits and compared to the pectin isolated from the paste resulting from the extraction of the olive oil after milling with malaxation at 30 °C/30 min and subsequent centrifugation of the olive paste from the same lot of olive fruits in a system called ABENCOR (AB).
34942961	5	43	theme	higher	980:985	arg1	content					987:993	a higher content	978:993	a higher content in AB extracts	978:1008	The polyphenols associated mainly with the acidic pectin substance, with a higher content in AB extracts, seem to be responsible for these activities, and appear to maintain their activities in part after complexation.
34942961	2	44	theme	olive	327:331	arg1	fruits					333:338	fresh olive fruits	321:338	fresh olive fruits	321:338	For this, pectin was extracted from fresh olive fruits and compared to the pectin isolated from the paste resulting from the extraction of the olive oil after milling with malaxation at 30 °C/30 min and subsequent centrifugation of the olive paste from the same lot of olive fruits in a system called ABENCOR (AB).
34942961	4	45	theme	cell	860:863	arg1	lines					865:869	colon carcinoma Caco-2 cell lines	837:869	colon carcinoma Caco-2 cell lines	837:869	In addition, the resulting AB extracts exhibited high antioxidant activity (ORAC) and strong antiproliferative activity in vitro against colon carcinoma Caco-2 cell lines compared to olive fruit extracts.
34942961	2	46	from	min	480:482	arg1	fruits					560:565	olive fruits	554:565	olive fruits	554:565	For this, pectin was extracted from fresh olive fruits and compared to the pectin isolated from the paste resulting from the extraction of the olive oil after milling with malaxation at 30 °C/30 min and subsequent centrifugation of the olive paste from the same lot of olive fruits in a system called ABENCOR (AB).
34942961	2	46	from	min	480:482	arg1	lot					547:549	the same lot	538:549	the same lot of olive fruits in a system called ABENCOR (AB)	538:597	For this, pectin was extracted from fresh olive fruits and compared to the pectin isolated from the paste resulting from the extraction of the olive oil after milling with malaxation at 30 °C/30 min and subsequent centrifugation of the olive paste from the same lot of olive fruits in a system called ABENCOR (AB).
34942961	2	47	theme	fresh	321:325	arg1	fruits					333:338	fresh olive fruits	321:338	fresh olive fruits	321:338	For this, pectin was extracted from fresh olive fruits and compared to the pectin isolated from the paste resulting from the extraction of the olive oil after milling with malaxation at 30 °C/30 min and subsequent centrifugation of the olive paste from the same lot of olive fruits in a system called ABENCOR (AB).
34942961	4	48	theme	Caco-2	853:858	arg1	lines					865:869	colon carcinoma Caco-2 cell lines	837:869	colon carcinoma Caco-2 cell lines	837:869	In addition, the resulting AB extracts exhibited high antioxidant activity (ORAC) and strong antiproliferative activity in vitro against colon carcinoma Caco-2 cell lines compared to olive fruit extracts.
34942961	2	49	from	centrifugation	499:512	arg1	fruits					560:565	olive fruits	554:565	olive fruits	554:565	For this, pectin was extracted from fresh olive fruits and compared to the pectin isolated from the paste resulting from the extraction of the olive oil after milling with malaxation at 30 °C/30 min and subsequent centrifugation of the olive paste from the same lot of olive fruits in a system called ABENCOR (AB).
34942961	2	49	from	centrifugation	499:512	arg1	lot					547:549	the same lot	538:549	the same lot of olive fruits in a system called ABENCOR (AB)	538:597	For this, pectin was extracted from fresh olive fruits and compared to the pectin isolated from the paste resulting from the extraction of the olive oil after milling with malaxation at 30 °C/30 min and subsequent centrifugation of the olive paste from the same lot of olive fruits in a system called ABENCOR (AB).
34942961	4	50	theme	AB	727:728	arg1	extracts					730:737	the resulting AB extracts	713:737	the resulting AB extracts	713:737	In addition, the resulting AB extracts exhibited high antioxidant activity (ORAC) and strong antiproliferative activity in vitro against colon carcinoma Caco-2 cell lines compared to olive fruit extracts.
34942961	4	51	theme	fruit	889:893	arg1	extracts					895:902	olive fruit extracts	883:902	olive fruit extracts	883:902	In addition, the resulting AB extracts exhibited high antioxidant activity (ORAC) and strong antiproliferative activity in vitro against colon carcinoma Caco-2 cell lines compared to olive fruit extracts.
34942961	5	52	theme	AB	998:999	arg1	extracts					1001:1008	AB extracts	998:1008	AB extracts	998:1008	The polyphenols associated mainly with the acidic pectin substance, with a higher content in AB extracts, seem to be responsible for these activities, and appear to maintain their activities in part after complexation.
34942961	1	53	theme	pectic	221:226	arg1	polysaccharides					228:242	pectic polysaccharides	221:242	pectic polysaccharides	221:242	The aim of this research was to investigate the interaction of phenols and pectic polysaccharides during the olive oil extraction process.
34942961	2	54	theme	°C/30	474:478	arg1	min					480:482	30 °C/30 min	471:482	30 °C/30 min	471:482	For this, pectin was extracted from fresh olive fruits and compared to the pectin isolated from the paste resulting from the extraction of the olive oil after milling with malaxation at 30 °C/30 min and subsequent centrifugation of the olive paste from the same lot of olive fruits in a system called ABENCOR (AB).
34942961	2	55	attach	isolated	367:374	arg2	pectin					360:365	the pectin	356:365	the pectin isolated from the paste resulting from the extraction of the olive oil after milling with malaxation at 30 °C/30 min and subsequent centrifugation of the olive paste from the same lot of olive fruits in a system called ABENCOR (AB)	356:597	For this, pectin was extracted from fresh olive fruits and compared to the pectin isolated from the paste resulting from the extraction of the olive oil after milling with malaxation at 30 °C/30 min and subsequent centrifugation of the olive paste from the same lot of olive fruits in a system called ABENCOR (AB).
34942961	2	55	attach	isolated	367:374	arg1	paste					385:389	the paste	381:389	the paste resulting from the extraction of the olive oil after milling with malaxation at 30 °C/30 min and subsequent centrifugation of the olive paste from the same lot of olive fruits in a system called ABENCOR (AB)	381:597	For this, pectin was extracted from fresh olive fruits and compared to the pectin isolated from the paste resulting from the extraction of the olive oil after milling with malaxation at 30 °C/30 min and subsequent centrifugation of the olive paste from the same lot of olive fruits in a system called ABENCOR (AB).
34942961	4	56	theme	high	749:752	arg1	activity					766:773	high antioxidant activity	749:773	high antioxidant activity (ORAC)	749:780	In addition, the resulting AB extracts exhibited high antioxidant activity (ORAC) and strong antiproliferative activity in vitro against colon carcinoma Caco-2 cell lines compared to olive fruit extracts.
34942961	4	56	theme	high	749:752	arg1	ORAC					776:779	ORAC	776:779	ORAC	776:779	In addition, the resulting AB extracts exhibited high antioxidant activity (ORAC) and strong antiproliferative activity in vitro against colon carcinoma Caco-2 cell lines compared to olive fruit extracts.
34942961	4	57	theme	colon	837:841	arg1	lines					865:869	colon carcinoma Caco-2 cell lines	837:869	colon carcinoma Caco-2 cell lines	837:869	In addition, the resulting AB extracts exhibited high antioxidant activity (ORAC) and strong antiproliferative activity in vitro against colon carcinoma Caco-2 cell lines compared to olive fruit extracts.
34942961	6	58	from	compositions	1203:1214	arg1	phenols					1186:1192	phenols	1186:1192	phenols in their compositions	1186:1214	However, even in olive fruit extracts with smaller amounts of phenols in their compositions, pectic polysaccharides may also be involved in antioxidant and antiproliferative activities.
34942961	6	58	from	compositions	1203:1214	arg1	amounts					1175:1181	smaller amounts	1167:1181	smaller amounts of phenols in their compositions	1167:1214	However, even in olive fruit extracts with smaller amounts of phenols in their compositions, pectic polysaccharides may also be involved in antioxidant and antiproliferative activities.
34942961	1	59	theme	polysaccharides	228:242	arg1	interaction					194:204	the interaction	190:204	the interaction of phenols and pectic polysaccharides	190:242	The aim of this research was to investigate the interaction of phenols and pectic polysaccharides during the olive oil extraction process.
34942961	2	60	from	lot	547:549	arg1	system					572:577	a system	570:577	a system called ABENCOR (AB)	570:597	For this, pectin was extracted from fresh olive fruits and compared to the pectin isolated from the paste resulting from the extraction of the olive oil after milling with malaxation at 30 °C/30 min and subsequent centrifugation of the olive paste from the same lot of olive fruits in a system called ABENCOR (AB).
34942961	2	60	from	lot	547:549	arg1	paste					527:531	the olive paste	517:531	the olive paste from the same lot of olive fruits in a system called ABENCOR (AB)	517:597	For this, pectin was extracted from fresh olive fruits and compared to the pectin isolated from the paste resulting from the extraction of the olive oil after milling with malaxation at 30 °C/30 min and subsequent centrifugation of the olive paste from the same lot of olive fruits in a system called ABENCOR (AB).
34942961	2	60	from	lot	547:549	arg1	min					480:482	30 °C/30 min	471:482	30 °C/30 min	471:482	For this, pectin was extracted from fresh olive fruits and compared to the pectin isolated from the paste resulting from the extraction of the olive oil after milling with malaxation at 30 °C/30 min and subsequent centrifugation of the olive paste from the same lot of olive fruits in a system called ABENCOR (AB).
34942961	2	60	from	lot	547:549	arg1	centrifugation					499:512	subsequent centrifugation	488:512	subsequent centrifugation	488:512	For this, pectin was extracted from fresh olive fruits and compared to the pectin isolated from the paste resulting from the extraction of the olive oil after milling with malaxation at 30 °C/30 min and subsequent centrifugation of the olive paste from the same lot of olive fruits in a system called ABENCOR (AB).
34942961	6	61	theme	smaller	1167:1173	arg1	phenols					1186:1192	phenols	1186:1192	phenols in their compositions	1186:1214	However, even in olive fruit extracts with smaller amounts of phenols in their compositions, pectic polysaccharides may also be involved in antioxidant and antiproliferative activities.
34942961	6	61	theme	smaller	1167:1173	arg1	amounts					1175:1181	smaller amounts	1167:1181	smaller amounts of phenols in their compositions	1167:1214	However, even in olive fruit extracts with smaller amounts of phenols in their compositions, pectic polysaccharides may also be involved in antioxidant and antiproliferative activities.
34942961	2	62	theme	subsequent	488:497	arg1	centrifugation					499:512	subsequent centrifugation	488:512	subsequent centrifugation	488:512	For this, pectin was extracted from fresh olive fruits and compared to the pectin isolated from the paste resulting from the extraction of the olive oil after milling with malaxation at 30 °C/30 min and subsequent centrifugation of the olive paste from the same lot of olive fruits in a system called ABENCOR (AB).
33091493	0	0	dep	leaves	90:95	arg1	collected					97:105	collected	97:105	leaves collected in central-western Brazil	90:131	Variation in essential oil components and anti-inflammatory activity of Allophylus edulis leaves collected in central-western Brazil.
33091493	2	1	theme	disorders	288:296	arg1	treatment					264:272	popular treatment	256:272	popular treatment of intestinal disorders	256:296	is used for popular treatment of intestinal disorders and as an anti-inflammatory throat treatment.
33091493	12	2	theme	A.	2001:2002	arg1	edulis					2004:2009	A. edulis	2001:2009	A. edulis	2001:2009	This finding corroborates the use of A. edulis as a medicinal plant and indicates its potential in the therapy of inflammatory conditions.
33091493	9	3	theme	caryophyllene	1578:1590	arg1	oxide					1592:1596	caryophyllene oxide	1578:1596	caryophyllene oxide (29.5%)	1578:1604	RESULTS Both EOAE-D and EOAE-B showed sesquiterpenes as a major constituent, namely, caryophyllene oxide (29.5%) and α-zingiberene (45.0%), respectively.
33091493	9	3	theme	caryophyllene	1578:1590	arg1	%					1603:1603	29.5%	1599:1603	29.5%	1599:1603	RESULTS Both EOAE-D and EOAE-B showed sesquiterpenes as a major constituent, namely, caryophyllene oxide (29.5%) and α-zingiberene (45.0%), respectively.
33091493	4	4	theme	chemical	637:644	arg1	profile					646:652	the chemical profile	633:652	the chemical profile of the essential oil of A. edulis plants collected in Dourados (EOAE-D) and Bonito (EOAE-B), two cities in Mato Grosso do Sul State, Brazil	633:792	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of A. edulis plants collected in Dourados (EOAE-D) and Bonito (EOAE-B), two cities in Mato Grosso do Sul State, Brazil.
33091493	2	5	theme	anti-inflammatory	308:324	arg1	treatment					333:341	an anti-inflammatory throat treatment	305:341	an anti-inflammatory throat treatment	305:341	is used for popular treatment of intestinal disorders and as an anti-inflammatory throat treatment.
33091493	1	6	theme	chal-chal	206:214	arg1	"					215:215	"chal-chal"	205:215	"chal-chal"	205:215	ETHNOPHARMACOLOGICAL RELEVANCE An infusion obtained from the leaves of "chal-chal" (Allophylus edulis Radlk.)
33091493	7	7	theme	chromatographic	1268:1282	arg1	methods					1284:1290	chromatographic methods	1268:1290	chromatographic methods	1268:1290	The composition of the essential oil (EOAE-D and EOAE-B) was determined by GC/MS, and major compounds (caryophyllene oxide and α-zingiberene) were isolated and identified by chromatographic methods and NMR spectroscopy.
33091493	11	8	from	cities	1859:1864	arg1	samples					1838:1844	samples	1838:1844	samples from the two cities	1838:1864	CONCLUSIONS The results indicate that samples from the two cities differed in chemical composition but not in their anti-inflammatory and antihyperalgesic effects.
33091493	12	9	theme	medicinal	2016:2024	arg1	plant					2026:2030	a medicinal plant	2014:2030	a medicinal plant	2014:2030	This finding corroborates the use of A. edulis as a medicinal plant and indicates its potential in the therapy of inflammatory conditions.
33091493	5	10	dep	in	970:971	arg1	vivo					973:976	vivo	973:976	vivo	973:976	Additionally, we evaluated the anti-inflammatory effects of the essential oil, as well as that of the major compounds (caryophyllene oxide and α-zingiberene), in experimental in vivo models of inflammation in mice.
33091493	4	11	theme	edulis	681:686	arg1	plants					688:693	A. edulis plants	678:693	A. edulis plants collected in Dourados (EOAE-D) and Bonito (EOAE-B), two cities in Mato Grosso do Sul State, Brazil	678:792	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of A. edulis plants collected in Dourados (EOAE-D) and Bonito (EOAE-B), two cities in Mato Grosso do Sul State, Brazil.
33091493	10	12	theme	caryophyllene	1663:1675	arg1	oxide					1677:1681	caryophyllene oxide	1663:1681	caryophyllene oxide	1663:1681	In tests, EOAE, caryophyllene oxide and α-zingiberene-induced antiedematogenic and antihyperalgesic effects were found in the different utilized models.
33091493	1	13	theme	ETHNOPHARMACOLOGICAL	134:153	arg1	RELEVANCE					155:163	ETHNOPHARMACOLOGICAL RELEVANCE	134:163	ETHNOPHARMACOLOGICAL RELEVANCE An infusion obtained from the leaves of "chal-chal" (Allophylus edulis Radlk.)	134:242	ETHNOPHARMACOLOGICAL RELEVANCE An infusion obtained from the leaves of "chal-chal" (Allophylus edulis Radlk.)
33091493	8	14	theme	classical	1367:1375	arg1	models					1377:1382	two classical models	1363:1382	two classical models of inflammatory models	1363:1405	Anti-inflammatory capacities were assessed using two classical models of inflammatory models, carrageenan- and CFA-induced paw inflammation (mechanical and thermal hyperalgesia).
33091493	8	14	theme	classical	1367:1375	arg1	inflammation					1441:1452	paw inflammation	1437:1452	carrageenan- and CFA-induced paw inflammation (mechanical and thermal hyperalgesia)	1408:1490	Anti-inflammatory capacities were assessed using two classical models of inflammatory models, carrageenan- and CFA-induced paw inflammation (mechanical and thermal hyperalgesia).
33091493	5	15	theme	experimental	957:968	arg1	models					978:983	experimental in vivo models	957:983	experimental in vivo models of inflammation in mice	957:1007	Additionally, we evaluated the anti-inflammatory effects of the essential oil, as well as that of the major compounds (caryophyllene oxide and α-zingiberene), in experimental in vivo models of inflammation in mice.
33091493	10	16	theme	antihyperalgesic	1730:1745	arg1	effects					1747:1753	α-zingiberene-induced antiedematogenic and antihyperalgesic effects	1687:1753	α-zingiberene-induced antiedematogenic and antihyperalgesic effects	1687:1753	In tests, EOAE, caryophyllene oxide and α-zingiberene-induced antiedematogenic and antihyperalgesic effects were found in the different utilized models.
33091493	5	17	theme	essential	859:867	arg1	oil					869:871	the essential oil	855:871	the essential oil	855:871	Additionally, we evaluated the anti-inflammatory effects of the essential oil, as well as that of the major compounds (caryophyllene oxide and α-zingiberene), in experimental in vivo models of inflammation in mice.
33091493	9	18	dep	RESULTS	1493:1499	arg1	EOAE-B					1517:1522	EOAE-B	1517:1522	EOAE-B	1517:1522	RESULTS Both EOAE-D and EOAE-B showed sesquiterpenes as a major constituent, namely, caryophyllene oxide (29.5%) and α-zingiberene (45.0%), respectively.
33091493	9	18	dep	RESULTS	1493:1499	arg1	EOAE-D					1506:1511	EOAE-D	1506:1511	EOAE-D	1506:1511	RESULTS Both EOAE-D and EOAE-B showed sesquiterpenes as a major constituent, namely, caryophyllene oxide (29.5%) and α-zingiberene (45.0%), respectively.
33091493	9	18	dep	RESULTS	1493:1499	arg1	RESULTS					1493:1499	RESULTS Both EOAE-D and EOAE-B	1493:1522	RESULTS Both EOAE-D and EOAE-B	1493:1522	RESULTS Both EOAE-D and EOAE-B showed sesquiterpenes as a major constituent, namely, caryophyllene oxide (29.5%) and α-zingiberene (45.0%), respectively.
33091493	7	19	theme	oil	1127:1129	arg1	composition					1098:1108	The composition	1094:1108	The composition of the essential oil (EOAE-D and EOAE-B)	1094:1149	The composition of the essential oil (EOAE-D and EOAE-B) was determined by GC/MS, and major compounds (caryophyllene oxide and α-zingiberene) were isolated and identified by chromatographic methods and NMR spectroscopy.
33091493	0	20	theme	essential	13:21	arg1	components					27:36	essential oil components	13:36	essential oil components	13:36	Variation in essential oil components and anti-inflammatory activity of Allophylus edulis leaves collected in central-western Brazil.
33091493	8	21	theme	models	1400:1405	arg1	models					1377:1382	two classical models	1363:1382	two classical models of inflammatory models	1363:1405	Anti-inflammatory capacities were assessed using two classical models of inflammatory models, carrageenan- and CFA-induced paw inflammation (mechanical and thermal hyperalgesia).
33091493	8	21	theme	models	1400:1405	arg1	inflammation					1441:1452	paw inflammation	1437:1452	carrageenan- and CFA-induced paw inflammation (mechanical and thermal hyperalgesia)	1408:1490	Anti-inflammatory capacities were assessed using two classical models of inflammatory models, carrageenan- and CFA-induced paw inflammation (mechanical and thermal hyperalgesia).
33091493	0	22	theme	edulis	83:88	arg1	components					27:36	essential oil components	13:36	essential oil components	13:36	Variation in essential oil components and anti-inflammatory activity of Allophylus edulis leaves collected in central-western Brazil.
33091493	0	22	theme	edulis	83:88	arg1	activity					60:67	anti-inflammatory activity	42:67	anti-inflammatory activity of Allophylus edulis	42:88	Variation in essential oil components and anti-inflammatory activity of Allophylus edulis leaves collected in central-western Brazil.
33091493	5	23	theme	anti-inflammatory	826:842	arg1	effects					844:850	the anti-inflammatory effects	822:850	the anti-inflammatory effects of the essential oil, as well as that of the major compounds (caryophyllene oxide and α-zingiberene), in experimental in vivo models of inflammation in mice	822:1007	Additionally, we evaluated the anti-inflammatory effects of the essential oil, as well as that of the major compounds (caryophyllene oxide and α-zingiberene), in experimental in vivo models of inflammation in mice.
33091493	3	24	theme	medicinal	377:385	arg1	use					392:394	the anti-inflammatory medicinal folk use	355:394	the anti-inflammatory medicinal folk use	355:394	Because of the anti-inflammatory medicinal folk use, a previous work reported scientific research confirming the anti-inflammatory activity of A. edulis essential oil collected in Dourados, MS, Brazil, in March 2015.
33091493	8	25	theme	carrageenan-	1408:1419	arg1	models					1377:1382	two classical models	1363:1382	two classical models of inflammatory models	1363:1405	Anti-inflammatory capacities were assessed using two classical models of inflammatory models, carrageenan- and CFA-induced paw inflammation (mechanical and thermal hyperalgesia).
33091493	8	25	theme	carrageenan-	1408:1419	arg1	hyperalgesia					1478:1489	mechanical and thermal hyperalgesia	1455:1489	mechanical and thermal hyperalgesia	1455:1489	Anti-inflammatory capacities were assessed using two classical models of inflammatory models, carrageenan- and CFA-induced paw inflammation (mechanical and thermal hyperalgesia).
33091493	8	25	theme	carrageenan-	1408:1419	arg1	inflammation					1441:1452	paw inflammation	1437:1452	carrageenan- and CFA-induced paw inflammation (mechanical and thermal hyperalgesia)	1408:1490	Anti-inflammatory capacities were assessed using two classical models of inflammatory models, carrageenan- and CFA-induced paw inflammation (mechanical and thermal hyperalgesia).
33091493	1	26	dep	RELEVANCE	155:163	arg1	Radlk					236:240	Radlk	236:240	Radlk	236:240	ETHNOPHARMACOLOGICAL RELEVANCE An infusion obtained from the leaves of "chal-chal" (Allophylus edulis Radlk.)
33091493	1	26	dep	RELEVANCE	155:163	arg1	infusion					168:175	An infusion	165:175	ETHNOPHARMACOLOGICAL RELEVANCE An infusion obtained from the leaves of "chal-chal" (Allophylus edulis Radlk.)	134:242	ETHNOPHARMACOLOGICAL RELEVANCE An infusion obtained from the leaves of "chal-chal" (Allophylus edulis Radlk.)
33091493	5	27	from	inflammation	988:999	arg1	mice					1004:1007	mice	1004:1007	mice	1004:1007	Additionally, we evaluated the anti-inflammatory effects of the essential oil, as well as that of the major compounds (caryophyllene oxide and α-zingiberene), in experimental in vivo models of inflammation in mice.
33091493	5	28	theme	that	885:888	arg1	effects					844:850	the anti-inflammatory effects	822:850	the anti-inflammatory effects of the essential oil, as well as that of the major compounds (caryophyllene oxide and α-zingiberene), in experimental in vivo models of inflammation in mice	822:1007	Additionally, we evaluated the anti-inflammatory effects of the essential oil, as well as that of the major compounds (caryophyllene oxide and α-zingiberene), in experimental in vivo models of inflammation in mice.
33091493	12	29	theme	edulis	2004:2009	arg1	use					1994:1996	the use	1990:1996	the use of A. edulis as a medicinal plant	1990:2030	This finding corroborates the use of A. edulis as a medicinal plant and indicates its potential in the therapy of inflammatory conditions.
33091493	8	30	theme	CFA-induced	1425:1435	arg1	models					1377:1382	two classical models	1363:1382	two classical models of inflammatory models	1363:1405	Anti-inflammatory capacities were assessed using two classical models of inflammatory models, carrageenan- and CFA-induced paw inflammation (mechanical and thermal hyperalgesia).
33091493	8	30	theme	CFA-induced	1425:1435	arg1	hyperalgesia					1478:1489	mechanical and thermal hyperalgesia	1455:1489	mechanical and thermal hyperalgesia	1455:1489	Anti-inflammatory capacities were assessed using two classical models of inflammatory models, carrageenan- and CFA-induced paw inflammation (mechanical and thermal hyperalgesia).
33091493	8	30	theme	CFA-induced	1425:1435	arg1	inflammation					1441:1452	paw inflammation	1437:1452	carrageenan- and CFA-induced paw inflammation (mechanical and thermal hyperalgesia)	1408:1490	Anti-inflammatory capacities were assessed using two classical models of inflammatory models, carrageenan- and CFA-induced paw inflammation (mechanical and thermal hyperalgesia).
33091493	3	31	theme	anti-inflammatory	457:473	arg1	activity					475:482	the anti-inflammatory activity	453:482	the anti-inflammatory activity of A. edulis essential oil collected in Dourados, MS, Brazil, in March 2015	453:558	Because of the anti-inflammatory medicinal folk use, a previous work reported scientific research confirming the anti-inflammatory activity of A. edulis essential oil collected in Dourados, MS, Brazil, in March 2015.
33091493	0	32	theme	central-western	110:124	arg1	Brazil					126:131	central-western Brazil	110:131	central-western Brazil	110:131	Variation in essential oil components and anti-inflammatory activity of Allophylus edulis leaves collected in central-western Brazil.
33091493	0	33	theme	Allophylus	72:81	arg1	edulis					83:88	Allophylus edulis	72:88	Allophylus edulis	72:88	Variation in essential oil components and anti-inflammatory activity of Allophylus edulis leaves collected in central-western Brazil.
33091493	5	34	from	effects	844:850	arg1	models					978:983	experimental in vivo models	957:983	experimental in vivo models of inflammation in mice	957:1007	Additionally, we evaluated the anti-inflammatory effects of the essential oil, as well as that of the major compounds (caryophyllene oxide and α-zingiberene), in experimental in vivo models of inflammation in mice.
33091493	8	35	theme	mechanical	1455:1464	arg1	hyperalgesia					1478:1489	mechanical and thermal hyperalgesia	1455:1489	mechanical and thermal hyperalgesia	1455:1489	Anti-inflammatory capacities were assessed using two classical models of inflammatory models, carrageenan- and CFA-induced paw inflammation (mechanical and thermal hyperalgesia).
33091493	8	35	theme	mechanical	1455:1464	arg1	inflammation					1441:1452	paw inflammation	1437:1452	carrageenan- and CFA-induced paw inflammation (mechanical and thermal hyperalgesia)	1408:1490	Anti-inflammatory capacities were assessed using two classical models of inflammatory models, carrageenan- and CFA-induced paw inflammation (mechanical and thermal hyperalgesia).
33091493	3	36	theme	edulis	490:495	arg1	activity					475:482	the anti-inflammatory activity	453:482	the anti-inflammatory activity of A. edulis essential oil collected in Dourados, MS, Brazil, in March 2015	453:558	Because of the anti-inflammatory medicinal folk use, a previous work reported scientific research confirming the anti-inflammatory activity of A. edulis essential oil collected in Dourados, MS, Brazil, in March 2015.
33091493	5	37	from	mice	1004:1007	arg1	models					978:983	experimental in vivo models	957:983	experimental in vivo models of inflammation in mice	957:1007	Additionally, we evaluated the anti-inflammatory effects of the essential oil, as well as that of the major compounds (caryophyllene oxide and α-zingiberene), in experimental in vivo models of inflammation in mice.
33091493	7	38	theme	major	1180:1184	arg1	caryophyllene					1197:1209	caryophyllene oxide and α-zingiberene	1197:1233	caryophyllene oxide and α-zingiberene	1197:1233	The composition of the essential oil (EOAE-D and EOAE-B) was determined by GC/MS, and major compounds (caryophyllene oxide and α-zingiberene) were isolated and identified by chromatographic methods and NMR spectroscopy.
33091493	7	38	theme	major	1180:1184	arg1	compounds					1186:1194	major compounds	1180:1194	major compounds (caryophyllene oxide and α-zingiberene)	1180:1234	The composition of the essential oil (EOAE-D and EOAE-B) was determined by GC/MS, and major compounds (caryophyllene oxide and α-zingiberene) were isolated and identified by chromatographic methods and NMR spectroscopy.
33091493	3	39	theme	scientific	422:431	arg1	research					433:440	scientific research	422:440	scientific research confirming the anti-inflammatory activity of A. edulis essential oil collected in Dourados, MS, Brazil, in March 2015	422:558	Because of the anti-inflammatory medicinal folk use, a previous work reported scientific research confirming the anti-inflammatory activity of A. edulis essential oil collected in Dourados, MS, Brazil, in March 2015.
33091493	8	40	theme	thermal	1470:1476	arg1	hyperalgesia					1478:1489	mechanical and thermal hyperalgesia	1455:1489	mechanical and thermal hyperalgesia	1455:1489	Anti-inflammatory capacities were assessed using two classical models of inflammatory models, carrageenan- and CFA-induced paw inflammation (mechanical and thermal hyperalgesia).
33091493	8	40	theme	thermal	1470:1476	arg1	inflammation					1441:1452	paw inflammation	1437:1452	carrageenan- and CFA-induced paw inflammation (mechanical and thermal hyperalgesia)	1408:1490	Anti-inflammatory capacities were assessed using two classical models of inflammatory models, carrageenan- and CFA-induced paw inflammation (mechanical and thermal hyperalgesia).
33091493	6	41	dep	MATERIALS	1010:1018	arg1	Leaves					1032:1037	Leaves	1032:1037	Leaves	1032:1037	MATERIALS AND METHODS Leaves were collected from plants at both sites in July 2018.
33091493	0	42	theme	anti-inflammatory	42:58	arg1	activity					60:67	anti-inflammatory activity	42:67	anti-inflammatory activity of Allophylus edulis	42:88	Variation in essential oil components and anti-inflammatory activity of Allophylus edulis leaves collected in central-western Brazil.
33091493	10	43	theme	antiedematogenic	1709:1724	arg1	effects					1747:1753	α-zingiberene-induced antiedematogenic and antihyperalgesic effects	1687:1753	α-zingiberene-induced antiedematogenic and antihyperalgesic effects	1687:1753	In tests, EOAE, caryophyllene oxide and α-zingiberene-induced antiedematogenic and antihyperalgesic effects were found in the different utilized models.
33091493	10	44	theme	different	1773:1781	arg1	models					1792:1797	the different utilized models	1769:1797	the different utilized models	1769:1797	In tests, EOAE, caryophyllene oxide and α-zingiberene-induced antiedematogenic and antihyperalgesic effects were found in the different utilized models.
33091493	12	45	theme	inflammatory	2078:2089	arg1	conditions					2091:2100	inflammatory conditions	2078:2100	inflammatory conditions	2078:2100	This finding corroborates the use of A. edulis as a medicinal plant and indicates its potential in the therapy of inflammatory conditions.
33091493	9	46	theme	major	1551:1555	arg1	constituent					1557:1567	a major constituent	1549:1567	a major constituent	1549:1567	RESULTS Both EOAE-D and EOAE-B showed sesquiterpenes as a major constituent, namely, caryophyllene oxide (29.5%) and α-zingiberene (45.0%), respectively.
33091493	9	46	theme	major	1551:1555	arg1	sesquiterpenes					1531:1544	sesquiterpenes	1531:1544	sesquiterpenes	1531:1544	RESULTS Both EOAE-D and EOAE-B showed sesquiterpenes as a major constituent, namely, caryophyllene oxide (29.5%) and α-zingiberene (45.0%), respectively.
33091493	4	47	theme	STUDY	572:576	arg1	AIM					561:563	AIM	561:563	AIM OF THE STUDY	561:576	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of A. edulis plants collected in Dourados (EOAE-D) and Bonito (EOAE-B), two cities in Mato Grosso do Sul State, Brazil.
33091493	3	48	theme	A.	487:488	arg1	edulis					490:495	A. edulis essential oil	487:509	A. edulis essential oil collected in Dourados, MS, Brazil, in March 2015	487:558	Because of the anti-inflammatory medicinal folk use, a previous work reported scientific research confirming the anti-inflammatory activity of A. edulis essential oil collected in Dourados, MS, Brazil, in March 2015.
33091493	11	49	theme	anti-inflammatory	1916:1932	arg1	effects					1955:1961	their anti-inflammatory and antihyperalgesic effects	1910:1961	their anti-inflammatory and antihyperalgesic effects	1910:1961	CONCLUSIONS The results indicate that samples from the two cities differed in chemical composition but not in their anti-inflammatory and antihyperalgesic effects.
33091493	10	50	located	found	1760:1764	arg2	effects					1747:1753	α-zingiberene-induced antiedematogenic and antihyperalgesic effects	1687:1753	α-zingiberene-induced antiedematogenic and antihyperalgesic effects	1687:1753	In tests, EOAE, caryophyllene oxide and α-zingiberene-induced antiedematogenic and antihyperalgesic effects were found in the different utilized models.
33091493	10	50	located	found	1760:1764	arg2	EOAE					1657:1660	EOAE	1657:1660	EOAE	1657:1660	In tests, EOAE, caryophyllene oxide and α-zingiberene-induced antiedematogenic and antihyperalgesic effects were found in the different utilized models.
33091493	10	50	located	found	1760:1764	arg1	models					1792:1797	the different utilized models	1769:1797	the different utilized models	1769:1797	In tests, EOAE, caryophyllene oxide and α-zingiberene-induced antiedematogenic and antihyperalgesic effects were found in the different utilized models.
33091493	10	50	located	found	1760:1764	arg2	oxide					1677:1681	caryophyllene oxide	1663:1681	caryophyllene oxide	1663:1681	In tests, EOAE, caryophyllene oxide and α-zingiberene-induced antiedematogenic and antihyperalgesic effects were found in the different utilized models.
33091493	10	50	located	found	1760:1764	arg1	tests					1650:1654	tests	1650:1654	tests	1650:1654	In tests, EOAE, caryophyllene oxide and α-zingiberene-induced antiedematogenic and antihyperalgesic effects were found in the different utilized models.
33091493	2	51	theme	intestinal	277:286	arg1	disorders					288:296	intestinal disorders	277:296	intestinal disorders	277:296	is used for popular treatment of intestinal disorders and as an anti-inflammatory throat treatment.
33091493	2	52	theme	throat	326:331	arg1	treatment					333:341	an anti-inflammatory throat treatment	305:341	an anti-inflammatory throat treatment	305:341	is used for popular treatment of intestinal disorders and as an anti-inflammatory throat treatment.
33091493	7	53	theme	NMR	1296:1298	arg1	spectroscopy					1300:1311	NMR spectroscopy	1296:1311	NMR spectroscopy	1296:1311	The composition of the essential oil (EOAE-D and EOAE-B) was determined by GC/MS, and major compounds (caryophyllene oxide and α-zingiberene) were isolated and identified by chromatographic methods and NMR spectroscopy.
33091493	11	54	theme	antihyperalgesic	1938:1953	arg1	effects					1955:1961	their anti-inflammatory and antihyperalgesic effects	1910:1961	their anti-inflammatory and antihyperalgesic effects	1910:1961	CONCLUSIONS The results indicate that samples from the two cities differed in chemical composition but not in their anti-inflammatory and antihyperalgesic effects.
33091493	4	55	from	variation	620:628	arg1	profile					646:652	the chemical profile	633:652	the chemical profile of the essential oil of A. edulis plants collected in Dourados (EOAE-D) and Bonito (EOAE-B), two cities in Mato Grosso do Sul State, Brazil	633:792	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of A. edulis plants collected in Dourados (EOAE-D) and Bonito (EOAE-B), two cities in Mato Grosso do Sul State, Brazil.
33091493	4	56	dep	Grosso	766:771	arg1	do					773:774	do	773:774	do Sul State, Brazil	773:792	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of A. edulis plants collected in Dourados (EOAE-D) and Bonito (EOAE-B), two cities in Mato Grosso do Sul State, Brazil.
33091493	10	57	theme	α-zingiberene-induced	1687:1707	arg1	effects					1747:1753	α-zingiberene-induced antiedematogenic and antihyperalgesic effects	1687:1753	α-zingiberene-induced antiedematogenic and antihyperalgesic effects	1687:1753	In tests, EOAE, caryophyllene oxide and α-zingiberene-induced antiedematogenic and antihyperalgesic effects were found in the different utilized models.
33091493	5	58	dep	compounds	903:911	arg1	α-zingiberene					938:950	α-zingiberene	938:950	α-zingiberene	938:950	Additionally, we evaluated the anti-inflammatory effects of the essential oil, as well as that of the major compounds (caryophyllene oxide and α-zingiberene), in experimental in vivo models of inflammation in mice.
33091493	5	58	dep	compounds	903:911	arg1	compounds					903:911	the major compounds	893:911	the major compounds (caryophyllene oxide and α-zingiberene)	893:951	Additionally, we evaluated the anti-inflammatory effects of the essential oil, as well as that of the major compounds (caryophyllene oxide and α-zingiberene), in experimental in vivo models of inflammation in mice.
33091493	5	58	dep	compounds	903:911	arg1	oxide					928:932	caryophyllene oxide	914:932	caryophyllene oxide	914:932	Additionally, we evaluated the anti-inflammatory effects of the essential oil, as well as that of the major compounds (caryophyllene oxide and α-zingiberene), in experimental in vivo models of inflammation in mice.
33091493	5	59	theme	inflammation	988:999	arg1	models					978:983	experimental in vivo models	957:983	experimental in vivo models of inflammation in mice	957:1007	Additionally, we evaluated the anti-inflammatory effects of the essential oil, as well as that of the major compounds (caryophyllene oxide and α-zingiberene), in experimental in vivo models of inflammation in mice.
33091493	0	60	from	Variation	0:8	arg1	components					27:36	essential oil components	13:36	essential oil components	13:36	Variation in essential oil components and anti-inflammatory activity of Allophylus edulis leaves collected in central-western Brazil.
33091493	0	60	from	Variation	0:8	arg1	activity					60:67	anti-inflammatory activity	42:67	anti-inflammatory activity of Allophylus edulis	42:88	Variation in essential oil components and anti-inflammatory activity of Allophylus edulis leaves collected in central-western Brazil.
33091493	4	61	theme	A.	678:679	arg1	plants					688:693	A. edulis plants	678:693	A. edulis plants collected in Dourados (EOAE-D) and Bonito (EOAE-B), two cities in Mato Grosso do Sul State, Brazil	678:792	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of A. edulis plants collected in Dourados (EOAE-D) and Bonito (EOAE-B), two cities in Mato Grosso do Sul State, Brazil.
33091493	4	62	dep	was	600:602	arg1	AIM					561:563	AIM	561:563	AIM OF THE STUDY	561:576	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of A. edulis plants collected in Dourados (EOAE-D) and Bonito (EOAE-B), two cities in Mato Grosso do Sul State, Brazil.
33091493	4	63	from	cities	751:756	arg1	Grosso					766:771	Grosso	766:771	Grosso	766:771	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of A. edulis plants collected in Dourados (EOAE-D) and Bonito (EOAE-B), two cities in Mato Grosso do Sul State, Brazil.
33091493	8	64	theme	inflammatory	1387:1398	arg1	models					1400:1405	inflammatory models	1387:1405	inflammatory models	1387:1405	Anti-inflammatory capacities were assessed using two classical models of inflammatory models, carrageenan- and CFA-induced paw inflammation (mechanical and thermal hyperalgesia).
33091493	4	65	theme	plants	688:693	arg1	oil					671:673	the essential oil	657:673	the essential oil of A. edulis plants collected in Dourados (EOAE-D) and Bonito (EOAE-B), two cities in Mato Grosso do Sul State, Brazil	657:792	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of A. edulis plants collected in Dourados (EOAE-D) and Bonito (EOAE-B), two cities in Mato Grosso do Sul State, Brazil.
33091493	7	66	theme	essential	1117:1125	arg1	oil					1127:1129	the essential oil	1113:1129	the essential oil (EOAE-D and EOAE-B)	1113:1149	The composition of the essential oil (EOAE-D and EOAE-B) was determined by GC/MS, and major compounds (caryophyllene oxide and α-zingiberene) were isolated and identified by chromatographic methods and NMR spectroscopy.
33091493	5	67	theme	in	970:971	arg1	models					978:983	experimental in vivo models	957:983	experimental in vivo models of inflammation in mice	957:1007	Additionally, we evaluated the anti-inflammatory effects of the essential oil, as well as that of the major compounds (caryophyllene oxide and α-zingiberene), in experimental in vivo models of inflammation in mice.
33091493	11	68	theme	chemical	1878:1885	arg1	composition					1887:1897	chemical composition	1878:1897	chemical composition	1878:1897	CONCLUSIONS The results indicate that samples from the two cities differed in chemical composition but not in their anti-inflammatory and antihyperalgesic effects.
33091493	2	69	theme	popular	256:262	arg1	treatment					264:272	popular treatment	256:272	popular treatment of intestinal disorders	256:296	is used for popular treatment of intestinal disorders and as an anti-inflammatory throat treatment.
33091493	4	70	theme	oil	671:673	arg1	profile					646:652	the chemical profile	633:652	the chemical profile of the essential oil of A. edulis plants collected in Dourados (EOAE-D) and Bonito (EOAE-B), two cities in Mato Grosso do Sul State, Brazil	633:792	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of A. edulis plants collected in Dourados (EOAE-D) and Bonito (EOAE-B), two cities in Mato Grosso do Sul State, Brazil.
33091493	12	71	from	potential	2050:2058	arg1	therapy					2067:2073	the therapy	2063:2073	the therapy of inflammatory conditions	2063:2100	This finding corroborates the use of A. edulis as a medicinal plant and indicates its potential in the therapy of inflammatory conditions.
33091493	3	72	theme	folk	387:390	arg1	use					392:394	the anti-inflammatory medicinal folk use	355:394	the anti-inflammatory medicinal folk use	355:394	Because of the anti-inflammatory medicinal folk use, a previous work reported scientific research confirming the anti-inflammatory activity of A. edulis essential oil collected in Dourados, MS, Brazil, in March 2015.
33091493	8	73	theme	paw	1437:1439	arg1	models					1377:1382	two classical models	1363:1382	two classical models of inflammatory models	1363:1405	Anti-inflammatory capacities were assessed using two classical models of inflammatory models, carrageenan- and CFA-induced paw inflammation (mechanical and thermal hyperalgesia).
33091493	8	73	theme	paw	1437:1439	arg1	hyperalgesia					1478:1489	mechanical and thermal hyperalgesia	1455:1489	mechanical and thermal hyperalgesia	1455:1489	Anti-inflammatory capacities were assessed using two classical models of inflammatory models, carrageenan- and CFA-induced paw inflammation (mechanical and thermal hyperalgesia).
33091493	8	73	theme	paw	1437:1439	arg1	inflammation					1441:1452	paw inflammation	1437:1452	carrageenan- and CFA-induced paw inflammation (mechanical and thermal hyperalgesia)	1408:1490	Anti-inflammatory capacities were assessed using two classical models of inflammatory models, carrageenan- and CFA-induced paw inflammation (mechanical and thermal hyperalgesia).
33091493	6	74	from	sites	1074:1078	arg1	July					1083:1086	July	1083:1086	July	1083:1086	MATERIALS AND METHODS Leaves were collected from plants at both sites in July 2018.
33091493	3	75	theme	anti-inflammatory	359:375	arg1	use					392:394	the anti-inflammatory medicinal folk use	355:394	the anti-inflammatory medicinal folk use	355:394	Because of the anti-inflammatory medicinal folk use, a previous work reported scientific research confirming the anti-inflammatory activity of A. edulis essential oil collected in Dourados, MS, Brazil, in March 2015.
33091493	5	76	theme	oil	869:871	arg1	effects					844:850	the anti-inflammatory effects	822:850	the anti-inflammatory effects of the essential oil, as well as that of the major compounds (caryophyllene oxide and α-zingiberene), in experimental in vivo models of inflammation in mice	822:1007	Additionally, we evaluated the anti-inflammatory effects of the essential oil, as well as that of the major compounds (caryophyllene oxide and α-zingiberene), in experimental in vivo models of inflammation in mice.
33091493	3	77	theme	previous	399:406	arg1	work					408:411	a previous work	397:411	a previous work	397:411	Because of the anti-inflammatory medicinal folk use, a previous work reported scientific research confirming the anti-inflammatory activity of A. edulis essential oil collected in Dourados, MS, Brazil, in March 2015.
33091493	1	78	dep	the	191:193	arg1	leaves					195:200	leaves	195:200	leaves	195:200	ETHNOPHARMACOLOGICAL RELEVANCE An infusion obtained from the leaves of "chal-chal" (Allophylus edulis Radlk.)
33091493	0	79	theme	oil	23:25	arg1	components					27:36	essential oil components	13:36	essential oil components	13:36	Variation in essential oil components and anti-inflammatory activity of Allophylus edulis leaves collected in central-western Brazil.
33091493	1	80	dep	obtained	177:184	arg1	Radlk					236:240	Radlk	236:240	Radlk	236:240	ETHNOPHARMACOLOGICAL RELEVANCE An infusion obtained from the leaves of "chal-chal" (Allophylus edulis Radlk.)
33091493	1	80	dep	obtained	177:184	arg1	infusion					168:175	An infusion	165:175	ETHNOPHARMACOLOGICAL RELEVANCE An infusion obtained from the leaves of "chal-chal" (Allophylus edulis Radlk.)	134:242	ETHNOPHARMACOLOGICAL RELEVANCE An infusion obtained from the leaves of "chal-chal" (Allophylus edulis Radlk.)
33091493	3	81	theme	essential	497:505	arg1	oil					507:509	essential oil	497:509	A. edulis essential oil collected in Dourados, MS, Brazil, in March 2015	487:558	Because of the anti-inflammatory medicinal folk use, a previous work reported scientific research confirming the anti-inflammatory activity of A. edulis essential oil collected in Dourados, MS, Brazil, in March 2015.
33091493	11	82	dep	CONCLUSIONS	1800:1810	arg1	indicate					1824:1831	indicate	1824:1831	indicate that samples from the two cities differed in chemical composition but not in their anti-inflammatory and antihyperalgesic effects	1824:1961	CONCLUSIONS The results indicate that samples from the two cities differed in chemical composition but not in their anti-inflammatory and antihyperalgesic effects.
33091493	4	83	theme	essential	661:669	arg1	oil					671:673	the essential oil	657:673	the essential oil of A. edulis plants collected in Dourados (EOAE-D) and Bonito (EOAE-B), two cities in Mato Grosso do Sul State, Brazil	657:792	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of A. edulis plants collected in Dourados (EOAE-D) and Bonito (EOAE-B), two cities in Mato Grosso do Sul State, Brazil.
33091493	7	84	dep	oil	1127:1129	arg1	EOAE-B					1143:1148	EOAE-B	1143:1148	EOAE-B	1143:1148	The composition of the essential oil (EOAE-D and EOAE-B) was determined by GC/MS, and major compounds (caryophyllene oxide and α-zingiberene) were isolated and identified by chromatographic methods and NMR spectroscopy.
33091493	7	84	dep	oil	1127:1129	arg1	EOAE-D					1132:1137	EOAE-D	1132:1137	EOAE-D	1132:1137	The composition of the essential oil (EOAE-D and EOAE-B) was determined by GC/MS, and major compounds (caryophyllene oxide and α-zingiberene) were isolated and identified by chromatographic methods and NMR spectroscopy.
33091493	12	85	theme	conditions	2091:2100	arg1	therapy					2067:2073	the therapy	2063:2073	the therapy of inflammatory conditions	2063:2100	This finding corroborates the use of A. edulis as a medicinal plant and indicates its potential in the therapy of inflammatory conditions.
33091493	5	86	from	models	978:983	arg1	mice					1004:1007	mice	1004:1007	mice	1004:1007	Additionally, we evaluated the anti-inflammatory effects of the essential oil, as well as that of the major compounds (caryophyllene oxide and α-zingiberene), in experimental in vivo models of inflammation in mice.
33091493	7	87	dep	caryophyllene	1197:1209	arg1	α-zingiberene					1221:1233	α-zingiberene	1221:1233	α-zingiberene	1221:1233	The composition of the essential oil (EOAE-D and EOAE-B) was determined by GC/MS, and major compounds (caryophyllene oxide and α-zingiberene) were isolated and identified by chromatographic methods and NMR spectroscopy.
33091493	7	87	dep	caryophyllene	1197:1209	arg1	oxide					1211:1215	oxide	1211:1215	oxide	1211:1215	The composition of the essential oil (EOAE-D and EOAE-B) was determined by GC/MS, and major compounds (caryophyllene oxide and α-zingiberene) were isolated and identified by chromatographic methods and NMR spectroscopy.
33091493	5	88	theme	caryophyllene	914:926	arg1	compounds					903:911	the major compounds	893:911	the major compounds (caryophyllene oxide and α-zingiberene)	893:951	Additionally, we evaluated the anti-inflammatory effects of the essential oil, as well as that of the major compounds (caryophyllene oxide and α-zingiberene), in experimental in vivo models of inflammation in mice.
33091493	5	88	theme	caryophyllene	914:926	arg1	oxide					928:932	caryophyllene oxide	914:932	caryophyllene oxide	914:932	Additionally, we evaluated the anti-inflammatory effects of the essential oil, as well as that of the major compounds (caryophyllene oxide and α-zingiberene), in experimental in vivo models of inflammation in mice.
33091493	5	89	theme	major	897:901	arg1	α-zingiberene					938:950	α-zingiberene	938:950	α-zingiberene	938:950	Additionally, we evaluated the anti-inflammatory effects of the essential oil, as well as that of the major compounds (caryophyllene oxide and α-zingiberene), in experimental in vivo models of inflammation in mice.
33091493	5	89	theme	major	897:901	arg1	compounds					903:911	the major compounds	893:911	the major compounds (caryophyllene oxide and α-zingiberene)	893:951	Additionally, we evaluated the anti-inflammatory effects of the essential oil, as well as that of the major compounds (caryophyllene oxide and α-zingiberene), in experimental in vivo models of inflammation in mice.
33091493	5	89	theme	major	897:901	arg1	oxide					928:932	caryophyllene oxide	914:932	caryophyllene oxide	914:932	Additionally, we evaluated the anti-inflammatory effects of the essential oil, as well as that of the major compounds (caryophyllene oxide and α-zingiberene), in experimental in vivo models of inflammation in mice.
33091493	4	90	theme	study	594:598	arg1	aim					582:584	The aim	578:584	The aim of this study	578:598	AIM OF THE STUDY The aim of this study was to evaluate the variation in the chemical profile of the essential oil of A. edulis plants collected in Dourados (EOAE-D) and Bonito (EOAE-B), two cities in Mato Grosso do Sul State, Brazil.
33091493	10	91	theme	utilized	1783:1790	arg1	models					1792:1797	the different utilized models	1769:1797	the different utilized models	1769:1797	In tests, EOAE, caryophyllene oxide and α-zingiberene-induced antiedematogenic and antihyperalgesic effects were found in the different utilized models.
33091493	3	92	dep	edulis	490:495	arg1	oil					507:509	essential oil	497:509	A. edulis essential oil collected in Dourados, MS, Brazil, in March 2015	487:558	Because of the anti-inflammatory medicinal folk use, a previous work reported scientific research confirming the anti-inflammatory activity of A. edulis essential oil collected in Dourados, MS, Brazil, in March 2015.
33091493	8	93	theme	Anti-inflammatory	1314:1330	arg1	capacities					1332:1341	Anti-inflammatory capacities	1314:1341	Anti-inflammatory capacities	1314:1341	Anti-inflammatory capacities were assessed using two classical models of inflammatory models, carrageenan- and CFA-induced paw inflammation (mechanical and thermal hyperalgesia).
34873206	7	0	used	used	1299:1302	arg2	SF					1235:1236	SF	1235:1236	SF	1235:1236	The study concludes that SF is a potential biotherapeutic for breast cancer and could be used initially as an add-on therapy to other standard of care to provide improved efficacy without other adverse effects.
34873206	7	0	used	used	1299:1302	arg2	biotherapeutic					1253:1266	a potential biotherapeutic	1241:1266	a potential biotherapeutic for breast cancer	1241:1284	The study concludes that SF is a potential biotherapeutic for breast cancer and could be used initially as an add-on therapy to other standard of care to provide improved efficacy without other adverse effects.
34873206	7	0	used	used	1299:1302	arg2	therapy					1327:1333	an add-on therapy	1317:1333	an add-on therapy	1317:1333	The study concludes that SF is a potential biotherapeutic for breast cancer and could be used initially as an add-on therapy to other standard of care to provide improved efficacy without other adverse effects.
34873206	6	1	from	doses	1126:1130	arg1	injections					1089:1098	daily intra tumor injections	1071:1098	daily intra tumor injections at 50 mg/ml and 100 mg/ml doses	1071:1130	In vivo, the tumor showed no growth after daily intra tumor injections at 50 mg/ml and 100 mg/ml doses whereas substantial tumor growth occurred after saline intra tumor injection.
34873206	1	2	theme	mesenchymal	234:244	arg1	cell					251:254	derived mesenchymal stem cell	226:254	human adipose derived mesenchymal stem cell secretome formulation of varying concentration	212:301	In the present study, a protocol was developed for processing of human adipose derived mesenchymal stem cell secretome formulation of varying concentration.
34873206	2	3	theme	breast	520:525	arg1	cancer					527:532	triple negative breast cancer	504:532	triple negative breast cancer	504:532	Its molecular composition was evaluated, and its effectiveness in vitro using breast cancer cell lines, and in vivo in a nude mice breast cancer model was studied to determine its role in suppressing triple negative breast cancer in a dose dependent manner.
34873206	7	4	theme	adverse	1404:1410	arg1	effects					1412:1418	other adverse effects	1398:1418	other adverse effects	1398:1418	The study concludes that SF is a potential biotherapeutic for breast cancer and could be used initially as an add-on therapy to other standard of care to provide improved efficacy without other adverse effects.
34873206	5	5	theme	cancer	1015:1020	arg1	cells					1022:1026	CD44+/CD24-, MDR1+ and PDL-1+ cancer cells	985:1026	CD44+/CD24-, MDR1+ and PDL-1+ cancer cells	985:1026	70 mg/ml of SF showed 90 ± 10% apoptosis and significant decrease in CD44+/CD24-, MDR1+ and PDL-1+ cancer cells.
34873206	1	6	theme	present	154:160	arg1	study					162:166	the present study	150:166	the present study	150:166	In the present study, a protocol was developed for processing of human adipose derived mesenchymal stem cell secretome formulation of varying concentration.
34873206	1	7	theme	stem	246:249	arg1	cell					251:254	derived mesenchymal stem cell	226:254	human adipose derived mesenchymal stem cell secretome formulation of varying concentration	212:301	In the present study, a protocol was developed for processing of human adipose derived mesenchymal stem cell secretome formulation of varying concentration.
34873206	4	8	theme	normal	902:907	arg1	cells					909:913	normal cells	902:913	normal cells	902:913	The results showed significant cell kill when exposed to the secretome above 20 mg/ml at which concentration there was no toxicity to normal cells.
34873206	6	9	theme	substantial	1140:1150	arg1	growth					1158:1163	substantial tumor growth	1140:1163	substantial tumor growth	1140:1163	In vivo, the tumor showed no growth after daily intra tumor injections at 50 mg/ml and 100 mg/ml doses whereas substantial tumor growth occurred after saline intra tumor injection.
34873206	6	10	theme	100 mg/ml	1116:1124	arg1	doses					1126:1130	50 mg/ml and 100 mg/ml doses	1103:1130	50 mg/ml and 100 mg/ml doses	1103:1130	In vivo, the tumor showed no growth after daily intra tumor injections at 50 mg/ml and 100 mg/ml doses whereas substantial tumor growth occurred after saline intra tumor injection.
34873206	1	11	theme	cell	251:254	arg1	formulation					266:276	human adipose derived mesenchymal stem cell secretome formulation	212:276	human adipose derived mesenchymal stem cell secretome formulation of varying concentration	212:301	In the present study, a protocol was developed for processing of human adipose derived mesenchymal stem cell secretome formulation of varying concentration.
34873206	3	12	theme	secretome	674:682	arg1	effectiveness					653:665	the effectiveness	649:665	the effectiveness of the secretome both in monotherapy and in combination therapy along with paclitaxel	649:751	Because the secretome could have value as an add-on therapy along with a current drug, the effectiveness of the secretome both in monotherapy and in combination therapy along with paclitaxel was evaluated.
34873206	0	13	link	derived	8:14	arg1	cell					33:36	Adipose derived mesenchymal stem cell	0:36	Adipose derived mesenchymal stem cell	0:36	Adipose derived mesenchymal stem cell secretome formulation as a biotherapeutic to inhibit growth of drug resistant triple negative breast cancer.
34873206	1	14	theme	secretome	256:264	arg1	formulation					266:276	human adipose derived mesenchymal stem cell secretome formulation	212:276	human adipose derived mesenchymal stem cell secretome formulation of varying concentration	212:301	In the present study, a protocol was developed for processing of human adipose derived mesenchymal stem cell secretome formulation of varying concentration.
34873206	0	15	theme	resistant	106:114	arg1	cancer					139:144	drug resistant triple negative breast cancer	101:144	drug resistant triple negative breast cancer	101:144	Adipose derived mesenchymal stem cell secretome formulation as a biotherapeutic to inhibit growth of drug resistant triple negative breast cancer.
34873206	7	16	theme	other	1398:1402	arg1	effects					1412:1418	other adverse effects	1398:1418	other adverse effects	1398:1418	The study concludes that SF is a potential biotherapeutic for breast cancer and could be used initially as an add-on therapy to other standard of care to provide improved efficacy without other adverse effects.
34873206	7	17	theme	potential	1243:1251	arg1	SF					1235:1236	SF	1235:1236	SF	1235:1236	The study concludes that SF is a potential biotherapeutic for breast cancer and could be used initially as an add-on therapy to other standard of care to provide improved efficacy without other adverse effects.
34873206	7	17	theme	potential	1243:1251	arg1	biotherapeutic					1253:1266	a potential biotherapeutic	1241:1266	a potential biotherapeutic for breast cancer	1241:1284	The study concludes that SF is a potential biotherapeutic for breast cancer and could be used initially as an add-on therapy to other standard of care to provide improved efficacy without other adverse effects.
34873206	5	18	theme	SF	928:929	arg1	70 mg/ml					916:923	70 mg/ml	916:923	70 mg/ml of SF	916:929	70 mg/ml of SF showed 90 ± 10% apoptosis and significant decrease in CD44+/CD24-, MDR1+ and PDL-1+ cancer cells.
34873206	2	19	theme	cell	396:399	arg1	lines					401:405	breast cancer cell lines	382:405	breast cancer cell lines	382:405	Its molecular composition was evaluated, and its effectiveness in vitro using breast cancer cell lines, and in vivo in a nude mice breast cancer model was studied to determine its role in suppressing triple negative breast cancer in a dose dependent manner.
34873206	3	20	from	effectiveness	653:665	arg1	therapy					723:729	combination therapy	711:729	combination therapy along with paclitaxel	711:751	Because the secretome could have value as an add-on therapy along with a current drug, the effectiveness of the secretome both in monotherapy and in combination therapy along with paclitaxel was evaluated.
34873206	3	20	from	effectiveness	653:665	arg1	paclitaxel					742:751	paclitaxel	742:751	combination therapy along with paclitaxel	711:751	Because the secretome could have value as an add-on therapy along with a current drug, the effectiveness of the secretome both in monotherapy and in combination therapy along with paclitaxel was evaluated.
34873206	3	20	from	effectiveness	653:665	arg1	monotherapy					692:702	monotherapy	692:702	monotherapy	692:702	Because the secretome could have value as an add-on therapy along with a current drug, the effectiveness of the secretome both in monotherapy and in combination therapy along with paclitaxel was evaluated.
34873206	3	21	dep	along	731:735	arg1	with					737:740	with	737:740	with	737:740	Because the secretome could have value as an add-on therapy along with a current drug, the effectiveness of the secretome both in monotherapy and in combination therapy along with paclitaxel was evaluated.
34873206	7	22	theme	care	1356:1359	arg1	standard					1344:1351	standard	1344:1351	standard	1344:1351	The study concludes that SF is a potential biotherapeutic for breast cancer and could be used initially as an add-on therapy to other standard of care to provide improved efficacy without other adverse effects.
34873206	2	23	theme	cancer	389:394	arg1	lines					401:405	breast cancer cell lines	382:405	breast cancer cell lines	382:405	Its molecular composition was evaluated, and its effectiveness in vitro using breast cancer cell lines, and in vivo in a nude mice breast cancer model was studied to determine its role in suppressing triple negative breast cancer in a dose dependent manner.
34873206	0	24	theme	derived	8:14	arg1	cell					33:36	Adipose derived mesenchymal stem cell	0:36	Adipose derived mesenchymal stem cell	0:36	Adipose derived mesenchymal stem cell secretome formulation as a biotherapeutic to inhibit growth of drug resistant triple negative breast cancer.
34873206	6	25	theme	tumor	1193:1197	arg1	injection					1199:1207	saline intra tumor injection	1180:1207	saline intra tumor injection	1180:1207	In vivo, the tumor showed no growth after daily intra tumor injections at 50 mg/ml and 100 mg/ml doses whereas substantial tumor growth occurred after saline intra tumor injection.
34873206	1	26	link	derived	226:232	arg1	cell					251:254	derived mesenchymal stem cell	226:254	human adipose derived mesenchymal stem cell secretome formulation of varying concentration	212:301	In the present study, a protocol was developed for processing of human adipose derived mesenchymal stem cell secretome formulation of varying concentration.
34873206	2	27	theme	negative	511:518	arg1	cancer					527:532	triple negative breast cancer	504:532	triple negative breast cancer	504:532	Its molecular composition was evaluated, and its effectiveness in vitro using breast cancer cell lines, and in vivo in a nude mice breast cancer model was studied to determine its role in suppressing triple negative breast cancer in a dose dependent manner.
34873206	2	28	theme	breast	382:387	arg1	lines					401:405	breast cancer cell lines	382:405	breast cancer cell lines	382:405	Its molecular composition was evaluated, and its effectiveness in vitro using breast cancer cell lines, and in vivo in a nude mice breast cancer model was studied to determine its role in suppressing triple negative breast cancer in a dose dependent manner.
34873206	0	29	theme	Adipose	0:6	arg1	cell					33:36	Adipose derived mesenchymal stem cell	0:36	Adipose derived mesenchymal stem cell	0:36	Adipose derived mesenchymal stem cell secretome formulation as a biotherapeutic to inhibit growth of drug resistant triple negative breast cancer.
34873206	1	30	theme	formulation	266:276	arg1	processing					198:207	processing	198:207	processing of human adipose derived mesenchymal stem cell secretome formulation of varying concentration	198:301	In the present study, a protocol was developed for processing of human adipose derived mesenchymal stem cell secretome formulation of varying concentration.
34873206	6	31	theme	intra	1187:1191	arg1	injection					1199:1207	saline intra tumor injection	1180:1207	saline intra tumor injection	1180:1207	In vivo, the tumor showed no growth after daily intra tumor injections at 50 mg/ml and 100 mg/ml doses whereas substantial tumor growth occurred after saline intra tumor injection.
34873206	2	32	theme	molecular	308:316	arg1	composition					318:328	Its molecular composition	304:328	Its molecular composition	304:328	Its molecular composition was evaluated, and its effectiveness in vitro using breast cancer cell lines, and in vivo in a nude mice breast cancer model was studied to determine its role in suppressing triple negative breast cancer in a dose dependent manner.
34873206	0	33	theme	stem	28:31	arg1	cell					33:36	Adipose derived mesenchymal stem cell	0:36	Adipose derived mesenchymal stem cell	0:36	Adipose derived mesenchymal stem cell secretome formulation as a biotherapeutic to inhibit growth of drug resistant triple negative breast cancer.
34873206	0	34	theme	negative	123:130	arg1	cancer					139:144	drug resistant triple negative breast cancer	101:144	drug resistant triple negative breast cancer	101:144	Adipose derived mesenchymal stem cell secretome formulation as a biotherapeutic to inhibit growth of drug resistant triple negative breast cancer.
34873206	6	35	theme	intra	1077:1081	arg1	injections					1089:1098	daily intra tumor injections	1071:1098	daily intra tumor injections at 50 mg/ml and 100 mg/ml doses	1071:1130	In vivo, the tumor showed no growth after daily intra tumor injections at 50 mg/ml and 100 mg/ml doses whereas substantial tumor growth occurred after saline intra tumor injection.
34873206	6	36	theme	saline	1180:1185	arg1	injection					1199:1207	saline intra tumor injection	1180:1207	saline intra tumor injection	1180:1207	In vivo, the tumor showed no growth after daily intra tumor injections at 50 mg/ml and 100 mg/ml doses whereas substantial tumor growth occurred after saline intra tumor injection.
34873206	2	37	theme	in	412:413	arg1	model					449:453	in vivo in a nude mice breast cancer model	412:453	in vivo in a nude mice breast cancer model	412:453	Its molecular composition was evaluated, and its effectiveness in vitro using breast cancer cell lines, and in vivo in a nude mice breast cancer model was studied to determine its role in suppressing triple negative breast cancer in a dose dependent manner.
34873206	0	38	theme	mesenchymal	16:26	arg1	cell					33:36	Adipose derived mesenchymal stem cell	0:36	Adipose derived mesenchymal stem cell	0:36	Adipose derived mesenchymal stem cell secretome formulation as a biotherapeutic to inhibit growth of drug resistant triple negative breast cancer.
34873206	0	39	theme	triple	116:121	arg1	cancer					139:144	drug resistant triple negative breast cancer	101:144	drug resistant triple negative breast cancer	101:144	Adipose derived mesenchymal stem cell secretome formulation as a biotherapeutic to inhibit growth of drug resistant triple negative breast cancer.
34873206	3	40	theme	add-on	607:612	arg1	therapy					614:620	an add-on therapy	604:620	an add-on therapy along with a current drug	604:646	Because the secretome could have value as an add-on therapy along with a current drug, the effectiveness of the secretome both in monotherapy and in combination therapy along with paclitaxel was evaluated.
34873206	3	40	theme	add-on	607:612	arg1	value					595:599	value	595:599	value	595:599	Because the secretome could have value as an add-on therapy along with a current drug, the effectiveness of the secretome both in monotherapy and in combination therapy along with paclitaxel was evaluated.
34873206	6	41	theme	daily	1071:1075	arg1	injections					1089:1098	daily intra tumor injections	1071:1098	daily intra tumor injections at 50 mg/ml and 100 mg/ml doses	1071:1130	In vivo, the tumor showed no growth after daily intra tumor injections at 50 mg/ml and 100 mg/ml doses whereas substantial tumor growth occurred after saline intra tumor injection.
34873206	6	42	theme	tumor	1083:1087	arg1	injections					1089:1098	daily intra tumor injections	1071:1098	daily intra tumor injections at 50 mg/ml and 100 mg/ml doses	1071:1130	In vivo, the tumor showed no growth after daily intra tumor injections at 50 mg/ml and 100 mg/ml doses whereas substantial tumor growth occurred after saline intra tumor injection.
34873206	0	43	theme	cancer	139:144	arg1	growth					91:96	growth	91:96	growth of drug resistant triple negative breast cancer	91:144	Adipose derived mesenchymal stem cell secretome formulation as a biotherapeutic to inhibit growth of drug resistant triple negative breast cancer.
34873206	5	44	theme	significant	961:971	arg1	decrease					973:980	significant decrease	961:980	significant decrease	961:980	70 mg/ml of SF showed 90 ± 10% apoptosis and significant decrease in CD44+/CD24-, MDR1+ and PDL-1+ cancer cells.
34873206	2	45	theme	cancer	442:447	arg1	model					449:453	in vivo in a nude mice breast cancer model	412:453	in vivo in a nude mice breast cancer model	412:453	Its molecular composition was evaluated, and its effectiveness in vitro using breast cancer cell lines, and in vivo in a nude mice breast cancer model was studied to determine its role in suppressing triple negative breast cancer in a dose dependent manner.
34873206	0	46	theme	breast	132:137	arg1	cancer					139:144	drug resistant triple negative breast cancer	101:144	drug resistant triple negative breast cancer	101:144	Adipose derived mesenchymal stem cell secretome formulation as a biotherapeutic to inhibit growth of drug resistant triple negative breast cancer.
34873206	7	47	theme	breast	1272:1277	arg1	cancer					1279:1284	breast cancer	1272:1284	breast cancer	1272:1284	The study concludes that SF is a potential biotherapeutic for breast cancer and could be used initially as an add-on therapy to other standard of care to provide improved efficacy without other adverse effects.
34873206	7	48	theme	add-on	1320:1325	arg1	therapy					1327:1333	an add-on therapy	1317:1333	an add-on therapy	1317:1333	The study concludes that SF is a potential biotherapeutic for breast cancer and could be used initially as an add-on therapy to other standard of care to provide improved efficacy without other adverse effects.
34873206	7	48	theme	add-on	1320:1325	arg1	SF					1235:1236	SF	1235:1236	SF	1235:1236	The study concludes that SF is a potential biotherapeutic for breast cancer and could be used initially as an add-on therapy to other standard of care to provide improved efficacy without other adverse effects.
34873206	2	49	theme	breast	435:440	arg1	model					449:453	in vivo in a nude mice breast cancer model	412:453	in vivo in a nude mice breast cancer model	412:453	Its molecular composition was evaluated, and its effectiveness in vitro using breast cancer cell lines, and in vivo in a nude mice breast cancer model was studied to determine its role in suppressing triple negative breast cancer in a dose dependent manner.
34873206	2	50	theme	dependent	544:552	arg1	manner					554:559	a dose dependent manner	537:559	a dose dependent manner	537:559	Its molecular composition was evaluated, and its effectiveness in vitro using breast cancer cell lines, and in vivo in a nude mice breast cancer model was studied to determine its role in suppressing triple negative breast cancer in a dose dependent manner.
34873206	5	51	theme	MDR1+	998:1002	arg1	cells					1022:1026	CD44+/CD24-, MDR1+ and PDL-1+ cancer cells	985:1026	CD44+/CD24-, MDR1+ and PDL-1+ cancer cells	985:1026	70 mg/ml of SF showed 90 ± 10% apoptosis and significant decrease in CD44+/CD24-, MDR1+ and PDL-1+ cancer cells.
34873206	2	52	dep	in	412:413	arg1	vivo					415:418	vivo	415:418	vivo	415:418	Its molecular composition was evaluated, and its effectiveness in vitro using breast cancer cell lines, and in vivo in a nude mice breast cancer model was studied to determine its role in suppressing triple negative breast cancer in a dose dependent manner.
34873206	7	53	theme	improved	1372:1379	arg1	efficacy					1381:1388	improved efficacy	1372:1388	improved efficacy	1372:1388	The study concludes that SF is a potential biotherapeutic for breast cancer and could be used initially as an add-on therapy to other standard of care to provide improved efficacy without other adverse effects.
34873206	2	54	theme	triple	504:509	arg1	cancer					527:532	triple negative breast cancer	504:532	triple negative breast cancer	504:532	Its molecular composition was evaluated, and its effectiveness in vitro using breast cancer cell lines, and in vivo in a nude mice breast cancer model was studied to determine its role in suppressing triple negative breast cancer in a dose dependent manner.
34873206	3	55	theme	combination	711:721	arg1	therapy					723:729	combination therapy	711:729	combination therapy along with paclitaxel	711:751	Because the secretome could have value as an add-on therapy along with a current drug, the effectiveness of the secretome both in monotherapy and in combination therapy along with paclitaxel was evaluated.
34873206	5	56	theme	CD44+/CD24-	985:995	arg1	cells					1022:1026	CD44+/CD24-, MDR1+ and PDL-1+ cancer cells	985:1026	CD44+/CD24-, MDR1+ and PDL-1+ cancer cells	985:1026	70 mg/ml of SF showed 90 ± 10% apoptosis and significant decrease in CD44+/CD24-, MDR1+ and PDL-1+ cancer cells.
34873206	3	57	theme	current	635:641	arg1	drug					643:646	a current drug	633:646	a current drug	633:646	Because the secretome could have value as an add-on therapy along with a current drug, the effectiveness of the secretome both in monotherapy and in combination therapy along with paclitaxel was evaluated.
34873206	5	58	theme	90 ± 10	938:944	arg1	%					945:945	%	945:945	%	945:945	70 mg/ml of SF showed 90 ± 10% apoptosis and significant decrease in CD44+/CD24-, MDR1+ and PDL-1+ cancer cells.
34873206	6	59	theme	tumor	1152:1156	arg1	growth					1158:1163	substantial tumor growth	1140:1163	substantial tumor growth	1140:1163	In vivo, the tumor showed no growth after daily intra tumor injections at 50 mg/ml and 100 mg/ml doses whereas substantial tumor growth occurred after saline intra tumor injection.
34873206	5	60	theme	%	945:945	arg1	apoptosis					947:955	90 ± 10% apoptosis	938:955	90 ± 10% apoptosis	938:955	70 mg/ml of SF showed 90 ± 10% apoptosis and significant decrease in CD44+/CD24-, MDR1+ and PDL-1+ cancer cells.
34873206	1	61	theme	human	212:216	arg1	formulation					266:276	human adipose derived mesenchymal stem cell secretome formulation	212:276	human adipose derived mesenchymal stem cell secretome formulation of varying concentration	212:301	In the present study, a protocol was developed for processing of human adipose derived mesenchymal stem cell secretome formulation of varying concentration.
34873206	4	62	dep	concentration	863:875	arg1	there					877:881	there	877:881	there	877:881	The results showed significant cell kill when exposed to the secretome above 20 mg/ml at which concentration there was no toxicity to normal cells.
34873206	5	63	theme	PDL-1+	1008:1013	arg1	cells					1022:1026	CD44+/CD24-, MDR1+ and PDL-1+ cancer cells	985:1026	CD44+/CD24-, MDR1+ and PDL-1+ cancer cells	985:1026	70 mg/ml of SF showed 90 ± 10% apoptosis and significant decrease in CD44+/CD24-, MDR1+ and PDL-1+ cancer cells.
34873206	4	64	dep	significant	787:797	arg1	kill					804:807	kill	804:807	kill when exposed to the secretome above 20 mg/ml at which concentration there was no toxicity to normal cells	804:913	The results showed significant cell kill when exposed to the secretome above 20 mg/ml at which concentration there was no toxicity to normal cells.
34873206	6	65	theme	50 mg/ml	1103:1110	arg1	doses					1126:1130	50 mg/ml and 100 mg/ml doses	1103:1130	50 mg/ml and 100 mg/ml doses	1103:1130	In vivo, the tumor showed no growth after daily intra tumor injections at 50 mg/ml and 100 mg/ml doses whereas substantial tumor growth occurred after saline intra tumor injection.
34873206	1	66	theme	adipose	218:224	arg1	formulation					266:276	human adipose derived mesenchymal stem cell secretome formulation	212:276	human adipose derived mesenchymal stem cell secretome formulation of varying concentration	212:301	In the present study, a protocol was developed for processing of human adipose derived mesenchymal stem cell secretome formulation of varying concentration.
34873206	3	67	contain	have	590:593	arg2	value					595:599	value	595:599	value	595:599	Because the secretome could have value as an add-on therapy along with a current drug, the effectiveness of the secretome both in monotherapy and in combination therapy along with paclitaxel was evaluated.
34873206	3	67	contain	have	590:593	arg2	therapy					614:620	an add-on therapy	604:620	an add-on therapy along with a current drug	604:646	Because the secretome could have value as an add-on therapy along with a current drug, the effectiveness of the secretome both in monotherapy and in combination therapy along with paclitaxel was evaluated.
34873206	3	67	contain	have	590:593	arg1	secretome					574:582	the secretome	570:582	the secretome	570:582	Because the secretome could have value as an add-on therapy along with a current drug, the effectiveness of the secretome both in monotherapy and in combination therapy along with paclitaxel was evaluated.
34873206	2	68	theme	nude	425:428	arg1	mice					430:433	a nude mice	423:433	a nude mice	423:433	Its molecular composition was evaluated, and its effectiveness in vitro using breast cancer cell lines, and in vivo in a nude mice breast cancer model was studied to determine its role in suppressing triple negative breast cancer in a dose dependent manner.
34873206	1	69	theme	derived	226:232	arg1	cell					251:254	derived mesenchymal stem cell	226:254	human adipose derived mesenchymal stem cell secretome formulation of varying concentration	212:301	In the present study, a protocol was developed for processing of human adipose derived mesenchymal stem cell secretome formulation of varying concentration.
33427608	6	0	theme	fatty	1005:1009	arg1	acids					1011:1015	major cellular fatty acids	990:1015	major cellular fatty acids	990:1015	Cells contained C18 :1 ω9c, C18 : 0 and C16 : 0 as the major cellular fatty acids and MK-9 (H2) as the predominant respiratory quinone.
33427608	6	0	theme	fatty	1005:1009	arg1	C16 					975:978	C16 	975:978	C16 	975:978	Cells contained C18 :1 ω9c, C18 : 0 and C16 : 0 as the major cellular fatty acids and MK-9 (H2) as the predominant respiratory quinone.
33427608	6	0	theme	fatty	1005:1009	arg1	C18 					963:966	C18 	963:966	C18 	963:966	Cells contained C18 :1 ω9c, C18 : 0 and C16 : 0 as the major cellular fatty acids and MK-9 (H2) as the predominant respiratory quinone.
33427608	6	0	theme	fatty	1005:1009	arg1	ω9c					958:960	ω9c	958:960	ω9c	958:960	Cells contained C18 :1 ω9c, C18 : 0 and C16 : 0 as the major cellular fatty acids and MK-9 (H2) as the predominant respiratory quinone.
33427608	10	1	theme	digital	1488:1494	arg1	hybridization					1504:1516	digital DNA-DNA hybridization	1488:1516	digital DNA-DNA hybridization (dDDH)	1488:1523	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strains 23H37-10T and 4HC-13 were 94.4 and 99.6 %, respectively.
33427608	10	1	theme	digital	1488:1494	arg1	dDDH					1519:1522	dDDH	1519:1522	dDDH	1519:1522	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strains 23H37-10T and 4HC-13 were 94.4 and 99.6 %, respectively.
33427608	3	2	theme	0.5-1.5 	445:452	arg1	%					453:453	%	453:453	%	453:453	Optimal growth was observed at 35-37 °C, pH 7.0-8.0 and with 0.5-1.5 % (w/v) NaCl.
33427608	5	3	dep	%	726:726	arg1	97.2 					721:725	97.2 	721:725	97.2 	721:725	Phylogenetic and phylogenomic analyses indicated that strains 23H37-10T and 4HC-13 formed an independent cluster within the genus Corynebacterium and showed 98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to Corynebacterium urogenitale LMM 1652T, Corynebacterium urealyticum DSM 7109T, Corynebacterium falsenii DSM 44353T and Corynebacterium jeikeium NCTC 11913T, respectively.
33427608	5	3	dep	%	726:726	arg1	97.4					712:715	97.4	712:715	97.4	712:715	Phylogenetic and phylogenomic analyses indicated that strains 23H37-10T and 4HC-13 formed an independent cluster within the genus Corynebacterium and showed 98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to Corynebacterium urogenitale LMM 1652T, Corynebacterium urealyticum DSM 7109T, Corynebacterium falsenii DSM 44353T and Corynebacterium jeikeium NCTC 11913T, respectively.
33427608	7	4	theme	phosphatidyl	1166:1177	arg1	inositol					1179:1186	phosphatidyl inositol	1166:1186	phosphatidyl inositol mannosides	1166:1197	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidyl inositol mannosides, two unidentified phospholipids, four unidentified glycolipids and one unidentified lipid.
33427608	12	5	dep	Corynebacterium	2012:2026	arg1	anserum					2028:2034	anserum	2028:2034	anserum	2028:2034	The differential genotypic inferences, together with phenotypic and biochemical characteristics, suggested that strains 23H37-10T and 4HC-13 represent a novel species within the genus Corynebacterium, for which the name Corynebacterium anserum sp.
33427608	5	6	theme	Phylogenetic	543:554	arg1	analyses					573:580	Phylogenetic and phylogenomic analyses	543:580	Phylogenetic and phylogenomic analyses	543:580	Phylogenetic and phylogenomic analyses indicated that strains 23H37-10T and 4HC-13 formed an independent cluster within the genus Corynebacterium and showed 98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to Corynebacterium urogenitale LMM 1652T, Corynebacterium urealyticum DSM 7109T, Corynebacterium falsenii DSM 44353T and Corynebacterium jeikeium NCTC 11913T, respectively.
33427608	5	7	theme	16S	728:730	arg1	similarity					751:760	98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity	700:760	98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to Corynebacterium urogenitale LMM 1652T, Corynebacterium urealyticum DSM 7109T, Corynebacterium falsenii DSM 44353T and Corynebacterium jeikeium NCTC 11913T, respectively	700:932	Phylogenetic and phylogenomic analyses indicated that strains 23H37-10T and 4HC-13 formed an independent cluster within the genus Corynebacterium and showed 98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to Corynebacterium urogenitale LMM 1652T, Corynebacterium urealyticum DSM 7109T, Corynebacterium falsenii DSM 44353T and Corynebacterium jeikeium NCTC 11913T, respectively.
33427608	5	8	theme	LMM	793:795	arg1	1652T					797:801	Corynebacterium urogenitale LMM 1652T	765:801	Corynebacterium urogenitale LMM 1652T	765:801	Phylogenetic and phylogenomic analyses indicated that strains 23H37-10T and 4HC-13 formed an independent cluster within the genus Corynebacterium and showed 98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to Corynebacterium urogenitale LMM 1652T, Corynebacterium urealyticum DSM 7109T, Corynebacterium falsenii DSM 44353T and Corynebacterium jeikeium NCTC 11913T, respectively.
33427608	6	9	theme	respiratory	1050:1060	arg1	quinone					1062:1068	the predominant respiratory quinone	1034:1068	the predominant respiratory quinone	1034:1068	Cells contained C18 :1 ω9c, C18 : 0 and C16 : 0 as the major cellular fatty acids and MK-9 (H2) as the predominant respiratory quinone.
33427608	11	10	theme	%	1719:1719	arg1	values					1689:1694	dDDH and ANI values	1676:1694	dDDH and ANI values of less than 70 and 96 %	1676:1719	Strains 23H37-10T and 4HC-13 had dDDH and ANI values of less than 70 and 96 % with all available genomes of the genus Corynebacterium, respectively.
33427608	5	11	theme	phylogenomic	560:571	arg1	analyses					573:580	Phylogenetic and phylogenomic analyses	543:580	Phylogenetic and phylogenomic analyses	543:580	Phylogenetic and phylogenomic analyses indicated that strains 23H37-10T and 4HC-13 formed an independent cluster within the genus Corynebacterium and showed 98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to Corynebacterium urogenitale LMM 1652T, Corynebacterium urealyticum DSM 7109T, Corynebacterium falsenii DSM 44353T and Corynebacterium jeikeium NCTC 11913T, respectively.
33427608	9	12	theme	G+C	1425:1427	arg1	%					1481:1481	55.2 mol%	1473:1481	55.2 mol%	1473:1481	The genome G+C content of strains 23H37-10T and 4HC-13 was 55.2 mol%.
33427608	9	12	theme	G+C	1425:1427	arg1	content					1429:1435	The genome G+C content	1414:1435	The genome G+C content of strains 23H37-10T and 4HC-13	1414:1467	The genome G+C content of strains 23H37-10T and 4HC-13 was 55.2 mol%.
33427608	10	13	theme	nucleotide	1537:1546	arg1	ANI					1558:1560	ANI	1558:1560	ANI	1558:1560	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strains 23H37-10T and 4HC-13 were 94.4 and 99.6 %, respectively.
33427608	10	13	theme	nucleotide	1537:1546	arg1	identity					1548:1555	average nucleotide identity	1529:1555	average nucleotide identity (ANI)	1529:1561	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strains 23H37-10T and 4HC-13 were 94.4 and 99.6 %, respectively.
33427608	5	14	theme	independent	636:646	arg1	cluster					648:654	an independent cluster	633:654	an independent cluster	633:654	Phylogenetic and phylogenomic analyses indicated that strains 23H37-10T and 4HC-13 formed an independent cluster within the genus Corynebacterium and showed 98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to Corynebacterium urogenitale LMM 1652T, Corynebacterium urealyticum DSM 7109T, Corynebacterium falsenii DSM 44353T and Corynebacterium jeikeium NCTC 11913T, respectively.
33427608	7	15	theme	inositol	1179:1186	arg1	mannosides					1188:1197	phosphatidyl inositol mannosides	1166:1197	phosphatidyl inositol mannosides	1166:1197	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidyl inositol mannosides, two unidentified phospholipids, four unidentified glycolipids and one unidentified lipid.
33427608	7	15	theme	inositol	1179:1186	arg1	phospholipids					1217:1229	two unidentified phospholipids	1200:1229	two unidentified phospholipids	1200:1229	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidyl inositol mannosides, two unidentified phospholipids, four unidentified glycolipids and one unidentified lipid.
33427608	5	16	theme	Corynebacterium	765:779	arg1	1652T					797:801	Corynebacterium urogenitale LMM 1652T	765:801	Corynebacterium urogenitale LMM 1652T	765:801	Phylogenetic and phylogenomic analyses indicated that strains 23H37-10T and 4HC-13 formed an independent cluster within the genus Corynebacterium and showed 98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to Corynebacterium urogenitale LMM 1652T, Corynebacterium urealyticum DSM 7109T, Corynebacterium falsenii DSM 44353T and Corynebacterium jeikeium NCTC 11913T, respectively.
33427608	12	17	theme	Corynebacterium	2012:2026	arg1	sp					2036:2037	the name Corynebacterium anserum sp	2003:2037	the name Corynebacterium anserum sp	2003:2037	The differential genotypic inferences, together with phenotypic and biochemical characteristics, suggested that strains 23H37-10T and 4HC-13 represent a novel species within the genus Corynebacterium, for which the name Corynebacterium anserum sp.
33427608	5	18	theme	Corynebacterium	843:857	arg1	44353T					872:877	Corynebacterium falsenii DSM 44353T	843:877	Corynebacterium falsenii DSM 44353T	843:877	Phylogenetic and phylogenomic analyses indicated that strains 23H37-10T and 4HC-13 formed an independent cluster within the genus Corynebacterium and showed 98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to Corynebacterium urogenitale LMM 1652T, Corynebacterium urealyticum DSM 7109T, Corynebacterium falsenii DSM 44353T and Corynebacterium jeikeium NCTC 11913T, respectively.
33427608	5	19	theme	Corynebacterium	883:897	arg1	11913T					913:918	Corynebacterium jeikeium NCTC 11913T	883:918	Corynebacterium jeikeium NCTC 11913T	883:918	Phylogenetic and phylogenomic analyses indicated that strains 23H37-10T and 4HC-13 formed an independent cluster within the genus Corynebacterium and showed 98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to Corynebacterium urogenitale LMM 1652T, Corynebacterium urealyticum DSM 7109T, Corynebacterium falsenii DSM 44353T and Corynebacterium jeikeium NCTC 11913T, respectively.
33427608	11	20	theme	ANI	1685:1687	arg1	values					1689:1694	dDDH and ANI values	1676:1694	dDDH and ANI values of less than 70 and 96 %	1676:1719	Strains 23H37-10T and 4HC-13 had dDDH and ANI values of less than 70 and 96 % with all available genomes of the genus Corynebacterium, respectively.
33427608	5	21	theme	DSM	868:870	arg1	44353T					872:877	Corynebacterium falsenii DSM 44353T	843:877	Corynebacterium falsenii DSM 44353T	843:877	Phylogenetic and phylogenomic analyses indicated that strains 23H37-10T and 4HC-13 formed an independent cluster within the genus Corynebacterium and showed 98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to Corynebacterium urogenitale LMM 1652T, Corynebacterium urealyticum DSM 7109T, Corynebacterium falsenii DSM 44353T and Corynebacterium jeikeium NCTC 11913T, respectively.
33427608	11	22	contain	had	1672:1674	arg1	4HC-13					1665:1670	4HC-13	1665:1670	4HC-13	1665:1670	Strains 23H37-10T and 4HC-13 had dDDH and ANI values of less than 70 and 96 % with all available genomes of the genus Corynebacterium, respectively.
33427608	11	22	contain	had	1672:1674	arg1	Strains					1643:1649	Strains 23H37-10T and 4HC-13	1643:1670	Strains	1643:1649	Strains 23H37-10T and 4HC-13 had dDDH and ANI values of less than 70 and 96 % with all available genomes of the genus Corynebacterium, respectively.
33427608	11	22	contain	had	1672:1674	arg2	values					1689:1694	dDDH and ANI values	1676:1694	dDDH and ANI values of less than 70 and 96 %	1676:1719	Strains 23H37-10T and 4HC-13 had dDDH and ANI values of less than 70 and 96 % with all available genomes of the genus Corynebacterium, respectively.
33427608	5	23	dep	Corynebacterium	804:818	arg1	urealyticum					820:830	urealyticum	820:830	urealyticum	820:830	Phylogenetic and phylogenomic analyses indicated that strains 23H37-10T and 4HC-13 formed an independent cluster within the genus Corynebacterium and showed 98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to Corynebacterium urogenitale LMM 1652T, Corynebacterium urealyticum DSM 7109T, Corynebacterium falsenii DSM 44353T and Corynebacterium jeikeium NCTC 11913T, respectively.
33427608	5	24	dep	Corynebacterium	883:897	arg1	jeikeium					899:906	jeikeium	899:906	jeikeium	899:906	Phylogenetic and phylogenomic analyses indicated that strains 23H37-10T and 4HC-13 formed an independent cluster within the genus Corynebacterium and showed 98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to Corynebacterium urogenitale LMM 1652T, Corynebacterium urealyticum DSM 7109T, Corynebacterium falsenii DSM 44353T and Corynebacterium jeikeium NCTC 11913T, respectively.
33427608	11	25	theme	dDDH	1676:1679	arg1	values					1689:1694	dDDH and ANI values	1676:1694	dDDH and ANI values of less than 70 and 96 %	1676:1719	Strains 23H37-10T and 4HC-13 had dDDH and ANI values of less than 70 and 96 % with all available genomes of the genus Corynebacterium, respectively.
33427608	2	26	theme	bacterial	220:228	arg1	strains					230:236	coccoid-shaped bacterial strains	205:236	coccoid-shaped bacterial strains	205:236	Two Gram-stain-positive, facultatively aerobic, non-motile and rod- to coccoid-shaped bacterial strains, 23H37-10T and 4HC-13, were isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, Jiangxi Province, PR China.
33427608	2	26	theme	bacterial	220:228	arg1	4HC-13					253:258	4HC-13	253:258	4HC-13	253:258	Two Gram-stain-positive, facultatively aerobic, non-motile and rod- to coccoid-shaped bacterial strains, 23H37-10T and 4HC-13, were isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, Jiangxi Province, PR China.
33427608	2	26	theme	bacterial	220:228	arg1	23H37-10T					239:247	23H37-10T	239:247	23H37-10T	239:247	Two Gram-stain-positive, facultatively aerobic, non-motile and rod- to coccoid-shaped bacterial strains, 23H37-10T and 4HC-13, were isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, Jiangxi Province, PR China.
33427608	2	27	from	China	377:381	arg1	geese					316:320	greater white-fronted geese	294:320	greater white-fronted geese (Anser albifrons) at Poyang Lake, Jiangxi Province, PR China	294:381	Two Gram-stain-positive, facultatively aerobic, non-motile and rod- to coccoid-shaped bacterial strains, 23H37-10T and 4HC-13, were isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, Jiangxi Province, PR China.
33427608	2	27	from	China	377:381	arg1	albifrons					329:337	albifrons	329:337	albifrons	329:337	Two Gram-stain-positive, facultatively aerobic, non-motile and rod- to coccoid-shaped bacterial strains, 23H37-10T and 4HC-13, were isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, Jiangxi Province, PR China.
33427608	2	27	from	China	377:381	arg1	faeces					284:289	the faeces	280:289	the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, Jiangxi Province, PR China	280:381	Two Gram-stain-positive, facultatively aerobic, non-motile and rod- to coccoid-shaped bacterial strains, 23H37-10T and 4HC-13, were isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, Jiangxi Province, PR China.
33427608	2	28	from	faeces	284:289	arg1	China					377:381	Poyang Lake, Jiangxi Province, PR China	343:381	Poyang Lake, Jiangxi Province, PR China	343:381	Two Gram-stain-positive, facultatively aerobic, non-motile and rod- to coccoid-shaped bacterial strains, 23H37-10T and 4HC-13, were isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, Jiangxi Province, PR China.
33427608	8	29	contain	contained	1307:1315	arg2	hydrolysates					1400:1411	the major whole-cell hydrolysates	1379:1411	the major whole-cell hydrolysates	1379:1411	Strain 23H37-10T contained mycolic acids, with meso-diaminopimelic acid and arabinose as the major whole-cell hydrolysates.
33427608	8	29	contain	contained	1307:1315	arg1	23H37-10T					1297:1305	Strain 23H37-10T	1290:1305	Strain 23H37-10T	1290:1305	Strain 23H37-10T contained mycolic acids, with meso-diaminopimelic acid and arabinose as the major whole-cell hydrolysates.
33427608	8	29	contain	contained	1307:1315	arg2	acids					1325:1329	mycolic acids	1317:1329	mycolic acids	1317:1329	Strain 23H37-10T contained mycolic acids, with meso-diaminopimelic acid and arabinose as the major whole-cell hydrolysates.
33427608	5	30	theme	genus	667:671	arg1	Corynebacterium					673:687	the genus Corynebacterium	663:687	the genus Corynebacterium	663:687	Phylogenetic and phylogenomic analyses indicated that strains 23H37-10T and 4HC-13 formed an independent cluster within the genus Corynebacterium and showed 98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to Corynebacterium urogenitale LMM 1652T, Corynebacterium urealyticum DSM 7109T, Corynebacterium falsenii DSM 44353T and Corynebacterium jeikeium NCTC 11913T, respectively.
33427608	3	31	located	observed	403:410	arg1	7.0-8.0					428:434	pH 7.0-8.0	425:434	pH 7.0-8.0	425:434	Optimal growth was observed at 35-37 °C, pH 7.0-8.0 and with 0.5-1.5 % (w/v) NaCl.
33427608	3	31	located	observed	403:410	arg2	growth					392:397	Optimal growth	384:397	Optimal growth	384:397	Optimal growth was observed at 35-37 °C, pH 7.0-8.0 and with 0.5-1.5 % (w/v) NaCl.
33427608	3	31	located	observed	403:410	arg1	35-37 °C					415:422	35-37 °C	415:422	35-37 °C	415:422	Optimal growth was observed at 35-37 °C, pH 7.0-8.0 and with 0.5-1.5 % (w/v) NaCl.
33427608	8	32	theme	mycolic	1317:1323	arg1	acids					1325:1329	mycolic acids	1317:1329	mycolic acids	1317:1329	Strain 23H37-10T contained mycolic acids, with meso-diaminopimelic acid and arabinose as the major whole-cell hydrolysates.
33427608	8	32	theme	mycolic	1317:1323	arg1	hydrolysates					1400:1411	the major whole-cell hydrolysates	1379:1411	the major whole-cell hydrolysates	1379:1411	Strain 23H37-10T contained mycolic acids, with meso-diaminopimelic acid and arabinose as the major whole-cell hydrolysates.
33427608	3	33	theme	Optimal	384:390	arg1	growth					392:397	Optimal growth	384:397	Optimal growth	384:397	Optimal growth was observed at 35-37 °C, pH 7.0-8.0 and with 0.5-1.5 % (w/v) NaCl.
33427608	4	34	theme	strains	498:504	arg1	sequences					485:493	The 16S rRNA gene sequences	467:493	The 16S rRNA gene sequences of strains 23H37-10T and 4HC-13	467:525	The 16S rRNA gene sequences of strains 23H37-10T and 4HC-13 were identical.
33427608	4	34	theme	strains	498:504	arg1	identical					532:540	identical	532:540	identical	532:540	The 16S rRNA gene sequences of strains 23H37-10T and 4HC-13 were identical.
33427608	12	35	theme	genotypic	1809:1817	arg1	inferences					1819:1828	The differential genotypic inferences	1792:1828	The differential genotypic inferences	1792:1828	The differential genotypic inferences, together with phenotypic and biochemical characteristics, suggested that strains 23H37-10T and 4HC-13 represent a novel species within the genus Corynebacterium, for which the name Corynebacterium anserum sp.
33427608	1	36	theme	greater	62:68	arg1	geese					84:88	greater white-fronted geese	62:88	greater white-fronted geese (Anser albifrons)	62:106	nov., isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, PR China.
33427608	1	36	theme	greater	62:68	arg1	albifrons					97:105	albifrons	97:105	albifrons	97:105	nov., isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, PR China.
33427608	0	37	theme	Corynebacterium	0:14	arg1	sp					24:25	Corynebacterium anserum sp	0:25	Corynebacterium anserum sp.	0:26	Corynebacterium anserum sp.
33427608	1	38	theme	geese	84:88	arg1	faeces					52:57	the faeces	48:57	the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake	48:121	nov., isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, PR China.
33427608	7	39	theme	unidentified	1270:1281	arg1	lipid					1283:1287	one unidentified lipid	1266:1287	one unidentified lipid	1266:1287	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidyl inositol mannosides, two unidentified phospholipids, four unidentified glycolipids and one unidentified lipid.
33427608	8	40	theme	meso-diaminopimelic	1337:1355	arg1	acid					1357:1360	meso-diaminopimelic acid	1337:1360	meso-diaminopimelic acid	1337:1360	Strain 23H37-10T contained mycolic acids, with meso-diaminopimelic acid and arabinose as the major whole-cell hydrolysates.
33427608	5	41	theme	gene	737:740	arg1	similarity					751:760	98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity	700:760	98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to Corynebacterium urogenitale LMM 1652T, Corynebacterium urealyticum DSM 7109T, Corynebacterium falsenii DSM 44353T and Corynebacterium jeikeium NCTC 11913T, respectively	700:932	Phylogenetic and phylogenomic analyses indicated that strains 23H37-10T and 4HC-13 formed an independent cluster within the genus Corynebacterium and showed 98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to Corynebacterium urogenitale LMM 1652T, Corynebacterium urealyticum DSM 7109T, Corynebacterium falsenii DSM 44353T and Corynebacterium jeikeium NCTC 11913T, respectively.
33427608	9	42	theme	mol	1478:1480	arg1	%					1481:1481	55.2 mol%	1473:1481	55.2 mol%	1473:1481	The genome G+C content of strains 23H37-10T and 4HC-13 was 55.2 mol%.
33427608	9	42	theme	mol	1478:1480	arg1	content					1429:1435	The genome G+C content	1414:1435	The genome G+C content of strains 23H37-10T and 4HC-13	1414:1467	The genome G+C content of strains 23H37-10T and 4HC-13 was 55.2 mol%.
33427608	4	43	theme	rRNA	475:478	arg1	sequences					485:493	The 16S rRNA gene sequences	467:493	The 16S rRNA gene sequences of strains 23H37-10T and 4HC-13	467:525	The 16S rRNA gene sequences of strains 23H37-10T and 4HC-13 were identical.
33427608	4	43	theme	rRNA	475:478	arg1	identical					532:540	identical	532:540	identical	532:540	The 16S rRNA gene sequences of strains 23H37-10T and 4HC-13 were identical.
33427608	7	44	theme	polar	1075:1079	arg1	lipids					1081:1086	The polar lipids	1071:1086	The polar lipids	1071:1086	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidyl inositol mannosides, two unidentified phospholipids, four unidentified glycolipids and one unidentified lipid.
33427608	2	45	dep	Province	364:371	arg1	PR					374:375	PR	374:375	PR	374:375	Two Gram-stain-positive, facultatively aerobic, non-motile and rod- to coccoid-shaped bacterial strains, 23H37-10T and 4HC-13, were isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, Jiangxi Province, PR China.
33427608	2	46	theme	Province	364:371	arg1	China					377:381	Poyang Lake, Jiangxi Province, PR China	343:381	Poyang Lake, Jiangxi Province, PR China	343:381	Two Gram-stain-positive, facultatively aerobic, non-motile and rod- to coccoid-shaped bacterial strains, 23H37-10T and 4HC-13, were isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, Jiangxi Province, PR China.
33427608	7	47	theme	unidentified	1237:1248	arg1	glycolipids					1250:1260	four unidentified glycolipids	1232:1260	four unidentified glycolipids	1232:1260	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidyl inositol mannosides, two unidentified phospholipids, four unidentified glycolipids and one unidentified lipid.
33427608	2	48	theme	geese	316:320	arg1	faeces					284:289	the faeces	280:289	the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, Jiangxi Province, PR China	280:381	Two Gram-stain-positive, facultatively aerobic, non-motile and rod- to coccoid-shaped bacterial strains, 23H37-10T and 4HC-13, were isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, Jiangxi Province, PR China.
33427608	9	49	dep	strains	1440:1446	arg1	strains					1440:1446	strains 23H37-10T and 4HC-13	1440:1467	strains 23H37-10T and 4HC-13	1440:1467	The genome G+C content of strains 23H37-10T and 4HC-13 was 55.2 mol%.
33427608	9	49	dep	strains	1440:1446	arg1	4HC-13					1462:1467	4HC-13	1462:1467	4HC-13	1462:1467	The genome G+C content of strains 23H37-10T and 4HC-13 was 55.2 mol%.
33427608	9	49	dep	strains	1440:1446	arg1	23H37-10T					1448:1456	23H37-10T	1448:1456	23H37-10T	1448:1456	The genome G+C content of strains 23H37-10T and 4HC-13 was 55.2 mol%.
33427608	2	50	theme	greater	294:300	arg1	geese					316:320	greater white-fronted geese	294:320	greater white-fronted geese (Anser albifrons) at Poyang Lake, Jiangxi Province, PR China	294:381	Two Gram-stain-positive, facultatively aerobic, non-motile and rod- to coccoid-shaped bacterial strains, 23H37-10T and 4HC-13, were isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, Jiangxi Province, PR China.
33427608	2	50	theme	greater	294:300	arg1	albifrons					329:337	albifrons	329:337	albifrons	329:337	Two Gram-stain-positive, facultatively aerobic, non-motile and rod- to coccoid-shaped bacterial strains, 23H37-10T and 4HC-13, were isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, Jiangxi Province, PR China.
33427608	7	51	theme	unidentified	1204:1215	arg1	mannosides					1188:1197	phosphatidyl inositol mannosides	1166:1197	phosphatidyl inositol mannosides	1166:1197	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidyl inositol mannosides, two unidentified phospholipids, four unidentified glycolipids and one unidentified lipid.
33427608	7	51	theme	unidentified	1204:1215	arg1	phospholipids					1217:1229	two unidentified phospholipids	1200:1229	two unidentified phospholipids	1200:1229	The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidyl inositol mannosides, two unidentified phospholipids, four unidentified glycolipids and one unidentified lipid.
33427608	6	52	contain	contained	941:949	arg2	acids					1011:1015	major cellular fatty acids	990:1015	major cellular fatty acids	990:1015	Cells contained C18 :1 ω9c, C18 : 0 and C16 : 0 as the major cellular fatty acids and MK-9 (H2) as the predominant respiratory quinone.
33427608	6	52	contain	contained	941:949	arg2	C18 					963:966	C18 	963:966	C18 	963:966	Cells contained C18 :1 ω9c, C18 : 0 and C16 : 0 as the major cellular fatty acids and MK-9 (H2) as the predominant respiratory quinone.
33427608	6	52	contain	contained	941:949	arg2	C16 					975:978	C16 	975:978	C16 	975:978	Cells contained C18 :1 ω9c, C18 : 0 and C16 : 0 as the major cellular fatty acids and MK-9 (H2) as the predominant respiratory quinone.
33427608	6	52	contain	contained	941:949	arg1	Cells					935:939	Cells	935:939	Cells	935:939	Cells contained C18 :1 ω9c, C18 : 0 and C16 : 0 as the major cellular fatty acids and MK-9 (H2) as the predominant respiratory quinone.
33427608	6	52	contain	contained	941:949	arg2	ω9c					958:960	ω9c	958:960	ω9c	958:960	Cells contained C18 :1 ω9c, C18 : 0 and C16 : 0 as the major cellular fatty acids and MK-9 (H2) as the predominant respiratory quinone.
33427608	3	53	theme	%	453:453	arg1	NaCl					461:464	0.5-1.5 % (w/v) NaCl	445:464	0.5-1.5 % (w/v) NaCl	445:464	Optimal growth was observed at 35-37 °C, pH 7.0-8.0 and with 0.5-1.5 % (w/v) NaCl.
33427608	10	54	theme	DNA-DNA	1496:1502	arg1	hybridization					1504:1516	digital DNA-DNA hybridization	1488:1516	digital DNA-DNA hybridization (dDDH)	1488:1523	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strains 23H37-10T and 4HC-13 were 94.4 and 99.6 %, respectively.
33427608	10	54	theme	DNA-DNA	1496:1502	arg1	dDDH					1519:1522	dDDH	1519:1522	dDDH	1519:1522	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strains 23H37-10T and 4HC-13 were 94.4 and 99.6 %, respectively.
33427608	6	55	theme	cellular	996:1003	arg1	acids					1011:1015	major cellular fatty acids	990:1015	major cellular fatty acids	990:1015	Cells contained C18 :1 ω9c, C18 : 0 and C16 : 0 as the major cellular fatty acids and MK-9 (H2) as the predominant respiratory quinone.
33427608	6	55	theme	cellular	996:1003	arg1	C16 					975:978	C16 	975:978	C16 	975:978	Cells contained C18 :1 ω9c, C18 : 0 and C16 : 0 as the major cellular fatty acids and MK-9 (H2) as the predominant respiratory quinone.
33427608	6	55	theme	cellular	996:1003	arg1	C18 					963:966	C18 	963:966	C18 	963:966	Cells contained C18 :1 ω9c, C18 : 0 and C16 : 0 as the major cellular fatty acids and MK-9 (H2) as the predominant respiratory quinone.
33427608	6	55	theme	cellular	996:1003	arg1	ω9c					958:960	ω9c	958:960	ω9c	958:960	Cells contained C18 :1 ω9c, C18 : 0 and C16 : 0 as the major cellular fatty acids and MK-9 (H2) as the predominant respiratory quinone.
33427608	10	56	theme	average	1529:1535	arg1	ANI					1558:1560	ANI	1558:1560	ANI	1558:1560	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strains 23H37-10T and 4HC-13 were 94.4 and 99.6 %, respectively.
33427608	10	56	theme	average	1529:1535	arg1	identity					1548:1555	average nucleotide identity	1529:1555	average nucleotide identity (ANI)	1529:1561	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strains 23H37-10T and 4HC-13 were 94.4 and 99.6 %, respectively.
33427608	5	57	theme	DSM	832:834	arg1	7109T					836:840	Corynebacterium urealyticum DSM 7109T	804:840	Corynebacterium urealyticum DSM 7109T	804:840	Phylogenetic and phylogenomic analyses indicated that strains 23H37-10T and 4HC-13 formed an independent cluster within the genus Corynebacterium and showed 98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to Corynebacterium urogenitale LMM 1652T, Corynebacterium urealyticum DSM 7109T, Corynebacterium falsenii DSM 44353T and Corynebacterium jeikeium NCTC 11913T, respectively.
33427608	2	58	dep	Gram-stain-positive	138:156	arg1	aerobic					173:179	aerobic	173:179	aerobic	173:179	Two Gram-stain-positive, facultatively aerobic, non-motile and rod- to coccoid-shaped bacterial strains, 23H37-10T and 4HC-13, were isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, Jiangxi Province, PR China.
33427608	11	59	theme	Corynebacterium	1761:1775	arg1	genomes					1740:1746	all available genomes	1726:1746	all available genomes of the genus Corynebacterium	1726:1775	Strains 23H37-10T and 4HC-13 had dDDH and ANI values of less than 70 and 96 % with all available genomes of the genus Corynebacterium, respectively.
33427608	5	60	theme	urogenitale	781:791	arg1	1652T					797:801	Corynebacterium urogenitale LMM 1652T	765:801	Corynebacterium urogenitale LMM 1652T	765:801	Phylogenetic and phylogenomic analyses indicated that strains 23H37-10T and 4HC-13 formed an independent cluster within the genus Corynebacterium and showed 98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to Corynebacterium urogenitale LMM 1652T, Corynebacterium urealyticum DSM 7109T, Corynebacterium falsenii DSM 44353T and Corynebacterium jeikeium NCTC 11913T, respectively.
33427608	12	61	theme	novel	1945:1949	arg1	species					1951:1957	a novel species	1943:1957	a novel species	1943:1957	The differential genotypic inferences, together with phenotypic and biochemical characteristics, suggested that strains 23H37-10T and 4HC-13 represent a novel species within the genus Corynebacterium, for which the name Corynebacterium anserum sp.
33427608	12	62	dep	together	1831:1838	arg1	with					1840:1843	with	1840:1843	with	1840:1843	The differential genotypic inferences, together with phenotypic and biochemical characteristics, suggested that strains 23H37-10T and 4HC-13 represent a novel species within the genus Corynebacterium, for which the name Corynebacterium anserum sp.
33427608	9	63	theme	genome	1418:1423	arg1	%					1481:1481	55.2 mol%	1473:1481	55.2 mol%	1473:1481	The genome G+C content of strains 23H37-10T and 4HC-13 was 55.2 mol%.
33427608	9	63	theme	genome	1418:1423	arg1	content					1429:1435	The genome G+C content	1414:1435	The genome G+C content of strains 23H37-10T and 4HC-13	1414:1467	The genome G+C content of strains 23H37-10T and 4HC-13 was 55.2 mol%.
33427608	6	64	theme	predominant	1038:1048	arg1	quinone					1062:1068	the predominant respiratory quinone	1034:1068	the predominant respiratory quinone	1034:1068	Cells contained C18 :1 ω9c, C18 : 0 and C16 : 0 as the major cellular fatty acids and MK-9 (H2) as the predominant respiratory quinone.
33427608	10	65	theme	identity	1548:1555	arg1	values					1563:1568	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values	1484:1568	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strains 23H37-10T and 4HC-13	1484:1605	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strains 23H37-10T and 4HC-13 were 94.4 and 99.6 %, respectively.
33427608	10	65	theme	identity	1548:1555	arg1	%					1626:1626	94.4 and 99.6 %	1612:1626	%	1626:1626	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strains 23H37-10T and 4HC-13 were 94.4 and 99.6 %, respectively.
33427608	5	66	theme	sequence	742:749	arg1	similarity					751:760	98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity	700:760	98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to Corynebacterium urogenitale LMM 1652T, Corynebacterium urealyticum DSM 7109T, Corynebacterium falsenii DSM 44353T and Corynebacterium jeikeium NCTC 11913T, respectively	700:932	Phylogenetic and phylogenomic analyses indicated that strains 23H37-10T and 4HC-13 formed an independent cluster within the genus Corynebacterium and showed 98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to Corynebacterium urogenitale LMM 1652T, Corynebacterium urealyticum DSM 7109T, Corynebacterium falsenii DSM 44353T and Corynebacterium jeikeium NCTC 11913T, respectively.
33427608	2	67	attach	isolated	266:273	arg2	non-motile					182:191	non-motile	182:191	non-motile	182:191	Two Gram-stain-positive, facultatively aerobic, non-motile and rod- to coccoid-shaped bacterial strains, 23H37-10T and 4HC-13, were isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, Jiangxi Province, PR China.
33427608	2	67	attach	isolated	266:273	arg1	faeces					284:289	the faeces	280:289	the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, Jiangxi Province, PR China	280:381	Two Gram-stain-positive, facultatively aerobic, non-motile and rod- to coccoid-shaped bacterial strains, 23H37-10T and 4HC-13, were isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, Jiangxi Province, PR China.
33427608	2	67	attach	isolated	266:273	arg2	rod-					197:200	rod-	197:200	rod-	197:200	Two Gram-stain-positive, facultatively aerobic, non-motile and rod- to coccoid-shaped bacterial strains, 23H37-10T and 4HC-13, were isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, Jiangxi Province, PR China.
33427608	2	67	attach	isolated	266:273	arg2	Gram-stain-positive					138:156	Gram-stain-positive	138:156	Gram-stain-positive	138:156	Two Gram-stain-positive, facultatively aerobic, non-motile and rod- to coccoid-shaped bacterial strains, 23H37-10T and 4HC-13, were isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, Jiangxi Province, PR China.
33427608	14	68	theme	=GDMCC	2088:2093	arg1	21672T					2108:2113	=GDMCC 1.1737T=KACC 21672T	2088:2113	=GDMCC 1.1737T=KACC 21672T	2088:2113	The type strain is 23H37-10T (=GDMCC 1.1737T=KACC 21672T).
33427608	14	68	theme	=GDMCC	2088:2093	arg1	23H37-10T					2077:2085	23H37-10T	2077:2085	23H37-10T (=GDMCC 1.1737T=KACC 21672T)	2077:2114	The type strain is 23H37-10T (=GDMCC 1.1737T=KACC 21672T).
33427608	4	69	dep	strains	498:504	arg1	strains					498:504	strains 23H37-10T and 4HC-13	498:525	strains 23H37-10T and 4HC-13	498:525	The 16S rRNA gene sequences of strains 23H37-10T and 4HC-13 were identical.
33427608	4	69	dep	strains	498:504	arg1	4HC-13					520:525	4HC-13	520:525	4HC-13	520:525	The 16S rRNA gene sequences of strains 23H37-10T and 4HC-13 were identical.
33427608	4	69	dep	strains	498:504	arg1	23H37-10T					506:514	23H37-10T	506:514	23H37-10T	506:514	The 16S rRNA gene sequences of strains 23H37-10T and 4HC-13 were identical.
33427608	2	70	theme	coccoid-shaped	205:218	arg1	strains					230:236	coccoid-shaped bacterial strains	205:236	coccoid-shaped bacterial strains	205:236	Two Gram-stain-positive, facultatively aerobic, non-motile and rod- to coccoid-shaped bacterial strains, 23H37-10T and 4HC-13, were isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, Jiangxi Province, PR China.
33427608	2	70	theme	coccoid-shaped	205:218	arg1	4HC-13					253:258	4HC-13	253:258	4HC-13	253:258	Two Gram-stain-positive, facultatively aerobic, non-motile and rod- to coccoid-shaped bacterial strains, 23H37-10T and 4HC-13, were isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, Jiangxi Province, PR China.
33427608	2	70	theme	coccoid-shaped	205:218	arg1	23H37-10T					239:247	23H37-10T	239:247	23H37-10T	239:247	Two Gram-stain-positive, facultatively aerobic, non-motile and rod- to coccoid-shaped bacterial strains, 23H37-10T and 4HC-13, were isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, Jiangxi Province, PR China.
33427608	9	71	theme	strains	1440:1446	arg1	%					1481:1481	55.2 mol%	1473:1481	55.2 mol%	1473:1481	The genome G+C content of strains 23H37-10T and 4HC-13 was 55.2 mol%.
33427608	9	71	theme	strains	1440:1446	arg1	content					1429:1435	The genome G+C content	1414:1435	The genome G+C content of strains 23H37-10T and 4HC-13	1414:1467	The genome G+C content of strains 23H37-10T and 4HC-13 was 55.2 mol%.
33427608	12	72	theme	name	2007:2010	arg1	sp					2036:2037	the name Corynebacterium anserum sp	2003:2037	the name Corynebacterium anserum sp	2003:2037	The differential genotypic inferences, together with phenotypic and biochemical characteristics, suggested that strains 23H37-10T and 4HC-13 represent a novel species within the genus Corynebacterium, for which the name Corynebacterium anserum sp.
33427608	11	73	theme	available	1730:1738	arg1	genomes					1740:1746	all available genomes	1726:1746	all available genomes of the genus Corynebacterium	1726:1775	Strains 23H37-10T and 4HC-13 had dDDH and ANI values of less than 70 and 96 % with all available genomes of the genus Corynebacterium, respectively.
33427608	8	74	theme	major	1383:1387	arg1	acids					1325:1329	mycolic acids	1317:1329	mycolic acids	1317:1329	Strain 23H37-10T contained mycolic acids, with meso-diaminopimelic acid and arabinose as the major whole-cell hydrolysates.
33427608	8	74	theme	major	1383:1387	arg1	hydrolysates					1400:1411	the major whole-cell hydrolysates	1379:1411	the major whole-cell hydrolysates	1379:1411	Strain 23H37-10T contained mycolic acids, with meso-diaminopimelic acid and arabinose as the major whole-cell hydrolysates.
33427608	5	75	theme	Corynebacterium	804:818	arg1	7109T					836:840	Corynebacterium urealyticum DSM 7109T	804:840	Corynebacterium urealyticum DSM 7109T	804:840	Phylogenetic and phylogenomic analyses indicated that strains 23H37-10T and 4HC-13 formed an independent cluster within the genus Corynebacterium and showed 98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to Corynebacterium urogenitale LMM 1652T, Corynebacterium urealyticum DSM 7109T, Corynebacterium falsenii DSM 44353T and Corynebacterium jeikeium NCTC 11913T, respectively.
33427608	14	76	theme	1.1737T=KACC	2095:2106	arg1	21672T					2108:2113	=GDMCC 1.1737T=KACC 21672T	2088:2113	=GDMCC 1.1737T=KACC 21672T	2088:2113	The type strain is 23H37-10T (=GDMCC 1.1737T=KACC 21672T).
33427608	14	76	theme	1.1737T=KACC	2095:2106	arg1	23H37-10T					2077:2085	23H37-10T	2077:2085	23H37-10T (=GDMCC 1.1737T=KACC 21672T)	2077:2114	The type strain is 23H37-10T (=GDMCC 1.1737T=KACC 21672T).
33427608	12	77	theme	phenotypic	1845:1854	arg1	characteristics					1872:1886	phenotypic and biochemical characteristics	1845:1886	phenotypic and biochemical characteristics	1845:1886	The differential genotypic inferences, together with phenotypic and biochemical characteristics, suggested that strains 23H37-10T and 4HC-13 represent a novel species within the genus Corynebacterium, for which the name Corynebacterium anserum sp.
33427608	1	78	theme	PR	124:125	arg1	China					127:131	PR China	124:131	PR China	124:131	nov., isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, PR China.
33427608	14	79	theme	type	2062:2065	arg1	23H37-10T					2077:2085	23H37-10T	2077:2085	23H37-10T (=GDMCC 1.1737T=KACC 21672T)	2077:2114	The type strain is 23H37-10T (=GDMCC 1.1737T=KACC 21672T).
33427608	14	79	theme	type	2062:2065	arg1	strain					2067:2072	The type strain	2058:2072	The type strain	2058:2072	The type strain is 23H37-10T (=GDMCC 1.1737T=KACC 21672T).
33427608	12	80	theme	biochemical	1860:1870	arg1	characteristics					1872:1886	phenotypic and biochemical characteristics	1845:1886	phenotypic and biochemical characteristics	1845:1886	The differential genotypic inferences, together with phenotypic and biochemical characteristics, suggested that strains 23H37-10T and 4HC-13 represent a novel species within the genus Corynebacterium, for which the name Corynebacterium anserum sp.
33427608	1	81	from	Lake	118:121	arg1	faeces					52:57	the faeces	48:57	the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake	48:121	nov., isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, PR China.
33427608	5	82	theme	NCTC	908:911	arg1	11913T					913:918	Corynebacterium jeikeium NCTC 11913T	883:918	Corynebacterium jeikeium NCTC 11913T	883:918	Phylogenetic and phylogenomic analyses indicated that strains 23H37-10T and 4HC-13 formed an independent cluster within the genus Corynebacterium and showed 98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to Corynebacterium urogenitale LMM 1652T, Corynebacterium urealyticum DSM 7109T, Corynebacterium falsenii DSM 44353T and Corynebacterium jeikeium NCTC 11913T, respectively.
33427608	8	83	theme	Strain	1290:1295	arg1	23H37-10T					1297:1305	Strain 23H37-10T	1290:1305	Strain 23H37-10T	1290:1305	Strain 23H37-10T contained mycolic acids, with meso-diaminopimelic acid and arabinose as the major whole-cell hydrolysates.
33427608	1	84	attach	isolated	34:41	arg2	nov.					28:31	nov.	28:31	nov.	28:31	nov., isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, PR China.
33427608	1	84	attach	isolated	34:41	arg1	faeces					52:57	the faeces	48:57	the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake	48:121	nov., isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, PR China.
33427608	6	85	theme	major	990:994	arg1	acids					1011:1015	major cellular fatty acids	990:1015	major cellular fatty acids	990:1015	Cells contained C18 :1 ω9c, C18 : 0 and C16 : 0 as the major cellular fatty acids and MK-9 (H2) as the predominant respiratory quinone.
33427608	6	85	theme	major	990:994	arg1	C16 					975:978	C16 	975:978	C16 	975:978	Cells contained C18 :1 ω9c, C18 : 0 and C16 : 0 as the major cellular fatty acids and MK-9 (H2) as the predominant respiratory quinone.
33427608	6	85	theme	major	990:994	arg1	C18 					963:966	C18 	963:966	C18 	963:966	Cells contained C18 :1 ω9c, C18 : 0 and C16 : 0 as the major cellular fatty acids and MK-9 (H2) as the predominant respiratory quinone.
33427608	6	85	theme	major	990:994	arg1	ω9c					958:960	ω9c	958:960	ω9c	958:960	Cells contained C18 :1 ω9c, C18 : 0 and C16 : 0 as the major cellular fatty acids and MK-9 (H2) as the predominant respiratory quinone.
33427608	8	86	with	acids	1325:1329	arg1	arabinose					1366:1374	arabinose	1366:1374	arabinose	1366:1374	Strain 23H37-10T contained mycolic acids, with meso-diaminopimelic acid and arabinose as the major whole-cell hydrolysates.
33427608	8	86	with	acids	1325:1329	arg1	acid					1357:1360	meso-diaminopimelic acid	1337:1360	meso-diaminopimelic acid	1337:1360	Strain 23H37-10T contained mycolic acids, with meso-diaminopimelic acid and arabinose as the major whole-cell hydrolysates.
33427608	5	87	dep	Corynebacterium	843:857	arg1	falsenii					859:866	falsenii	859:866	falsenii	859:866	Phylogenetic and phylogenomic analyses indicated that strains 23H37-10T and 4HC-13 formed an independent cluster within the genus Corynebacterium and showed 98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to Corynebacterium urogenitale LMM 1652T, Corynebacterium urealyticum DSM 7109T, Corynebacterium falsenii DSM 44353T and Corynebacterium jeikeium NCTC 11913T, respectively.
33427608	0	88	theme	anserum	16:22	arg1	sp					24:25	Corynebacterium anserum sp	0:25	Corynebacterium anserum sp.	0:26	Corynebacterium anserum sp.
33427608	1	89	theme	white-fronted	70:82	arg1	geese					84:88	greater white-fronted geese	62:88	greater white-fronted geese (Anser albifrons)	62:106	nov., isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, PR China.
33427608	1	89	theme	white-fronted	70:82	arg1	albifrons					97:105	albifrons	97:105	albifrons	97:105	nov., isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, PR China.
33427608	4	90	theme	16S	471:473	arg1	sequences					485:493	The 16S rRNA gene sequences	467:493	The 16S rRNA gene sequences of strains 23H37-10T and 4HC-13	467:525	The 16S rRNA gene sequences of strains 23H37-10T and 4HC-13 were identical.
33427608	4	90	theme	16S	471:473	arg1	identical					532:540	identical	532:540	identical	532:540	The 16S rRNA gene sequences of strains 23H37-10T and 4HC-13 were identical.
33427608	12	91	dep	strains	1904:1910	arg1	23H37-10T					1912:1920	23H37-10T	1912:1920	23H37-10T	1912:1920	The differential genotypic inferences, together with phenotypic and biochemical characteristics, suggested that strains 23H37-10T and 4HC-13 represent a novel species within the genus Corynebacterium, for which the name Corynebacterium anserum sp.
33427608	12	91	dep	strains	1904:1910	arg1	strains					1904:1910	strains	1904:1910	strains 23H37-10T and 4HC-13	1904:1931	The differential genotypic inferences, together with phenotypic and biochemical characteristics, suggested that strains 23H37-10T and 4HC-13 represent a novel species within the genus Corynebacterium, for which the name Corynebacterium anserum sp.
33427608	12	91	dep	strains	1904:1910	arg1	4HC-13					1926:1931	4HC-13	1926:1931	4HC-13	1926:1931	The differential genotypic inferences, together with phenotypic and biochemical characteristics, suggested that strains 23H37-10T and 4HC-13 represent a novel species within the genus Corynebacterium, for which the name Corynebacterium anserum sp.
33427608	1	92	dep	China	127:131	arg1	nov.					28:31	nov.	28:31	nov.	28:31	nov., isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, PR China.
33427608	5	93	theme	rRNA	732:735	arg1	similarity					751:760	98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity	700:760	98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to Corynebacterium urogenitale LMM 1652T, Corynebacterium urealyticum DSM 7109T, Corynebacterium falsenii DSM 44353T and Corynebacterium jeikeium NCTC 11913T, respectively	700:932	Phylogenetic and phylogenomic analyses indicated that strains 23H37-10T and 4HC-13 formed an independent cluster within the genus Corynebacterium and showed 98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to Corynebacterium urogenitale LMM 1652T, Corynebacterium urealyticum DSM 7109T, Corynebacterium falsenii DSM 44353T and Corynebacterium jeikeium NCTC 11913T, respectively.
33427608	3	94	dep	%	453:453	arg1	w/v					456:458	w/v	456:458	w/v	456:458	Optimal growth was observed at 35-37 °C, pH 7.0-8.0 and with 0.5-1.5 % (w/v) NaCl.
33427608	4	95	theme	gene	480:483	arg1	sequences					485:493	The 16S rRNA gene sequences	467:493	The 16S rRNA gene sequences of strains 23H37-10T and 4HC-13	467:525	The 16S rRNA gene sequences of strains 23H37-10T and 4HC-13 were identical.
33427608	4	95	theme	gene	480:483	arg1	identical					532:540	identical	532:540	identical	532:540	The 16S rRNA gene sequences of strains 23H37-10T and 4HC-13 were identical.
33427608	10	96	theme	99.6 	1621:1625	arg1	values					1563:1568	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values	1484:1568	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strains 23H37-10T and 4HC-13	1484:1605	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strains 23H37-10T and 4HC-13 were 94.4 and 99.6 %, respectively.
33427608	10	96	theme	99.6 	1621:1625	arg1	%					1626:1626	94.4 and 99.6 %	1612:1626	%	1626:1626	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strains 23H37-10T and 4HC-13 were 94.4 and 99.6 %, respectively.
33427608	12	97	theme	differential	1796:1807	arg1	inferences					1819:1828	The differential genotypic inferences	1792:1828	The differential genotypic inferences	1792:1828	The differential genotypic inferences, together with phenotypic and biochemical characteristics, suggested that strains 23H37-10T and 4HC-13 represent a novel species within the genus Corynebacterium, for which the name Corynebacterium anserum sp.
33427608	10	98	theme	94.4	1612:1615	arg1	values					1563:1568	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values	1484:1568	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strains 23H37-10T and 4HC-13	1484:1605	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strains 23H37-10T and 4HC-13 were 94.4 and 99.6 %, respectively.
33427608	10	98	theme	94.4	1612:1615	arg1	%					1626:1626	94.4 and 99.6 %	1612:1626	%	1626:1626	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strains 23H37-10T and 4HC-13 were 94.4 and 99.6 %, respectively.
33427608	6	99	dep	acids	1011:1015	arg1	the					986:988	the	986:988	the	986:988	Cells contained C18 :1 ω9c, C18 : 0 and C16 : 0 as the major cellular fatty acids and MK-9 (H2) as the predominant respiratory quinone.
33427608	2	100	theme	white-fronted	302:314	arg1	geese					316:320	greater white-fronted geese	294:320	greater white-fronted geese (Anser albifrons) at Poyang Lake, Jiangxi Province, PR China	294:381	Two Gram-stain-positive, facultatively aerobic, non-motile and rod- to coccoid-shaped bacterial strains, 23H37-10T and 4HC-13, were isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, Jiangxi Province, PR China.
33427608	2	100	theme	white-fronted	302:314	arg1	albifrons					329:337	albifrons	329:337	albifrons	329:337	Two Gram-stain-positive, facultatively aerobic, non-motile and rod- to coccoid-shaped bacterial strains, 23H37-10T and 4HC-13, were isolated from the faeces of greater white-fronted geese (Anser albifrons) at Poyang Lake, Jiangxi Province, PR China.
33427608	5	101	dep	similarity	751:760	arg1	%					726:726	%	726:726	%	726:726	Phylogenetic and phylogenomic analyses indicated that strains 23H37-10T and 4HC-13 formed an independent cluster within the genus Corynebacterium and showed 98.8, 97.4, 97.4 and 97.2 % 16S rRNA gene sequence similarity to Corynebacterium urogenitale LMM 1652T, Corynebacterium urealyticum DSM 7109T, Corynebacterium falsenii DSM 44353T and Corynebacterium jeikeium NCTC 11913T, respectively.
33427608	3	102	theme	pH	425:426	arg1	7.0-8.0					428:434	pH 7.0-8.0	425:434	pH 7.0-8.0	425:434	Optimal growth was observed at 35-37 °C, pH 7.0-8.0 and with 0.5-1.5 % (w/v) NaCl.
33427608	3	102	theme	pH	425:426	arg1	35-37 °C					415:422	35-37 °C	415:422	35-37 °C	415:422	Optimal growth was observed at 35-37 °C, pH 7.0-8.0 and with 0.5-1.5 % (w/v) NaCl.
33427608	10	103	theme	hybridization	1504:1516	arg1	values					1563:1568	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values	1484:1568	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strains 23H37-10T and 4HC-13	1484:1605	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strains 23H37-10T and 4HC-13 were 94.4 and 99.6 %, respectively.
33427608	10	103	theme	hybridization	1504:1516	arg1	%					1626:1626	94.4 and 99.6 %	1612:1626	%	1626:1626	The digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strains 23H37-10T and 4HC-13 were 94.4 and 99.6 %, respectively.
33427608	8	104	theme	whole-cell	1389:1398	arg1	acids					1325:1329	mycolic acids	1317:1329	mycolic acids	1317:1329	Strain 23H37-10T contained mycolic acids, with meso-diaminopimelic acid and arabinose as the major whole-cell hydrolysates.
33427608	8	104	theme	whole-cell	1389:1398	arg1	hydrolysates					1400:1411	the major whole-cell hydrolysates	1379:1411	the major whole-cell hydrolysates	1379:1411	Strain 23H37-10T contained mycolic acids, with meso-diaminopimelic acid and arabinose as the major whole-cell hydrolysates.
33942129	6	0	from	%	1012:1012	arg1	analysis					847:854	logistic regression analysis	827:854	logistic regression analysis	827:854	In logistic regression analysis, testing BOS group as dependent variable with Gal-1 and 3 as independent variables, area under the receiver operating characteristics (AUROC) curve was 98.9% (NPV 90% and PPV 88.9%, p = 0.0003).
33942129	3	1	theme	lung	528:531	arg1	patients					544:551	Nineteen lung transplant patients	519:551	Nineteen lung transplant patients	519:551	MATERIALS AND METHODS Nineteen lung transplant patients [median age (IQR), 55 (45-62) years; 53% males] were enrolled in the study.
33942129	6	2	theme	BOS	865:867	arg1	group					869:873	BOS group	865:873	BOS group	865:873	In logistic regression analysis, testing BOS group as dependent variable with Gal-1 and 3 as independent variables, area under the receiver operating characteristics (AUROC) curve was 98.9% (NPV 90% and PPV 88.9%, p = 0.0003).
33942129	6	2	theme	BOS	865:867	arg1	variables					929:937	independent variables	917:937	independent variables	917:937	In logistic regression analysis, testing BOS group as dependent variable with Gal-1 and 3 as independent variables, area under the receiver operating characteristics (AUROC) curve was 98.9% (NPV 90% and PPV 88.9%, p = 0.0003).
33942129	8	3	theme	stable	1199:1204	arg1	patients					1206:1213	stable patients	1199:1213	stable patients	1199:1213	In stable patients were observed an inverse correlation of Gal-3 with DLCO% and KCO%, and between Gal-9 and KCO%.
33942129	9	4	with	patients	1394:1401	arg1	meaning					1434:1440	diagnostic and prognostic meaning	1408:1440	diagnostic and prognostic meaning	1408:1440	CONCLUSION Galectins-1, 3 and 9 are possible clinical biomarkers in lung transplant patients with diagnostic and prognostic meaning.
33942129	6	5	theme	regression	836:845	arg1	analysis					847:854	logistic regression analysis	827:854	logistic regression analysis	827:854	In logistic regression analysis, testing BOS group as dependent variable with Gal-1 and 3 as independent variables, area under the receiver operating characteristics (AUROC) curve was 98.9% (NPV 90% and PPV 88.9%, p = 0.0003).
33942129	4	6	theme	blood	640:644	arg1	concentrations					646:659	Peripheral blood concentrations	629:659	Peripheral blood concentrations of galectins-1, 3 and 9	629:683	Peripheral blood concentrations of galectins-1, 3 and 9 were determined with commercial ELISA kits.
33942129	8	7	with	correlation	1240:1250	arg1	%					1270:1270	DLCO%	1266:1270	DLCO%	1266:1270	In stable patients were observed an inverse correlation of Gal-3 with DLCO% and KCO%, and between Gal-9 and KCO%.
33942129	7	8	with	%	1158:1158	arg1	group					1071:1075	the stable LTX group	1056:1075	the stable LTX group as dependent variable and Gal-1, 3 and 9 as independent variables	1056:1141	With the stable LTX group as dependent variable and Gal-1, 3 and 9 as independent variables, AUROC was 92.6% (NPV 100% and PPV 90%, p = 0.0023).
33942129	6	9	theme	independent	917:927	arg1	group					869:873	BOS group	865:873	BOS group	865:873	In logistic regression analysis, testing BOS group as dependent variable with Gal-1 and 3 as independent variables, area under the receiver operating characteristics (AUROC) curve was 98.9% (NPV 90% and PPV 88.9%, p = 0.0003).
33942129	6	9	theme	independent	917:927	arg1	variables					929:937	independent variables	917:937	independent variables	917:937	In logistic regression analysis, testing BOS group as dependent variable with Gal-1 and 3 as independent variables, area under the receiver operating characteristics (AUROC) curve was 98.9% (NPV 90% and PPV 88.9%, p = 0.0003).
33942129	2	10	theme	preliminary	300:310	arg1	study					312:316	This preliminary study	295:316	This preliminary study	295:316	This preliminary study investigated serum galectins as clinical biomarkers in lung transplant patients with chronic lung allograft dysfunction (CLAD), phenotype bronchiolitis obliterans syndrome (BOS).
33942129	3	11	dep	%	592:592	arg1	males					594:598	males	594:598	53% males	590:598	MATERIALS AND METHODS Nineteen lung transplant patients [median age (IQR), 55 (45-62) years; 53% males] were enrolled in the study.
33942129	7	12	theme	stable	1060:1065	arg1	group					1071:1075	the stable LTX group	1056:1075	the stable LTX group as dependent variable and Gal-1, 3 and 9 as independent variables	1056:1141	With the stable LTX group as dependent variable and Gal-1, 3 and 9 as independent variables, AUROC was 92.6% (NPV 100% and PPV 90%, p = 0.0023).
33942129	8	13	theme	KCO	1276:1278	arg1	%					1279:1279	KCO%	1276:1279	KCO%	1276:1279	In stable patients were observed an inverse correlation of Gal-3 with DLCO% and KCO%, and between Gal-9 and KCO%.
33942129	3	14	dep	age	561:563	arg1	%					592:592	53% males	590:598	53% males	590:598	MATERIALS AND METHODS Nineteen lung transplant patients [median age (IQR), 55 (45-62) years; 53% males] were enrolled in the study.
33942129	9	15	dep	CONCLUSION	1310:1319	arg1	Galectins-1					1321:1331	Galectins-1	1321:1331	Galectins-1	1321:1331	CONCLUSION Galectins-1, 3 and 9 are possible clinical biomarkers in lung transplant patients with diagnostic and prognostic meaning.
33942129	9	15	dep	CONCLUSION	1310:1319	arg1	9					1340:1340	9	1340:1340	9	1340:1340	CONCLUSION Galectins-1, 3 and 9 are possible clinical biomarkers in lung transplant patients with diagnostic and prognostic meaning.
33942129	9	15	dep	CONCLUSION	1310:1319	arg1	3					1334:1334	3	1334:1334	3	1334:1334	CONCLUSION Galectins-1, 3 and 9 are possible clinical biomarkers in lung transplant patients with diagnostic and prognostic meaning.
33942129	6	16	theme	characteristics	974:988	arg1	curve					998:1002	the receiver operating characteristics (AUROC) curve	951:1002	the receiver operating characteristics (AUROC) curve	951:1002	In logistic regression analysis, testing BOS group as dependent variable with Gal-1 and 3 as independent variables, area under the receiver operating characteristics (AUROC) curve was 98.9% (NPV 90% and PPV 88.9%, p = 0.0003).
33942129	5	17	theme	stable	790:795	arg1	p = 0.0394					811:820	p = 0.0394	811:820	p = 0.0394	811:820	RESULTS Galectin-1 concentrations were higher in BOS than in stable LTX patients (p = 0.0394).
33942129	5	17	theme	stable	790:795	arg1	patients					801:808	stable LTX patients	790:808	stable LTX patients (p = 0.0394)	790:821	RESULTS Galectin-1 concentrations were higher in BOS than in stable LTX patients (p = 0.0394).
33942129	6	18	theme	receiver	955:962	arg1	curve					998:1002	the receiver operating characteristics (AUROC) curve	951:1002	the receiver operating characteristics (AUROC) curve	951:1002	In logistic regression analysis, testing BOS group as dependent variable with Gal-1 and 3 as independent variables, area under the receiver operating characteristics (AUROC) curve was 98.9% (NPV 90% and PPV 88.9%, p = 0.0003).
33942129	5	19	theme	Galectin-1	737:746	arg1	concentrations					748:761	RESULTS Galectin-1 concentrations	729:761	RESULTS Galectin-1 concentrations	729:761	RESULTS Galectin-1 concentrations were higher in BOS than in stable LTX patients (p = 0.0394).
33942129	11	20	theme	therapeutic	1563:1573	arg1	targets					1575:1581	new therapeutic targets	1559:1581	new therapeutic targets	1559:1581	The hypothesis that they could be new therapeutic targets in BOS patients is intriguing and also worth exploring.
33942129	1	21	from	present	148:154	arg1	glycoproteins					181:193	N-linked and O-linked glycoproteins	159:193	N-linked and O-linked glycoproteins	159:193	BACKGROUND Galectins are proteins that bind β-galactosides such as N-acetyllactosamine present in N-linked and O-linked glycoproteins and that seem to be implicated in inflammatory and immune responses as well as fibrotic mechanisms.
33942129	3	22	dep	MATERIALS	497:505	arg1	years					583:587	55 (45-62) years	572:587	55 (45-62) years	572:587	MATERIALS AND METHODS Nineteen lung transplant patients [median age (IQR), 55 (45-62) years; 53% males] were enrolled in the study.
33942129	3	22	dep	MATERIALS	497:505	arg1	IQR					566:568	IQR	566:568	IQR	566:568	MATERIALS AND METHODS Nineteen lung transplant patients [median age (IQR), 55 (45-62) years; 53% males] were enrolled in the study.
33942129	3	22	dep	MATERIALS	497:505	arg1	age					561:563	[median age	553:563	[median age (IQR)	553:569	MATERIALS AND METHODS Nineteen lung transplant patients [median age (IQR), 55 (45-62) years; 53% males] were enrolled in the study.
33942129	3	22	dep	MATERIALS	497:505	arg1	patients					544:551	Nineteen lung transplant patients	519:551	Nineteen lung transplant patients	519:551	MATERIALS AND METHODS Nineteen lung transplant patients [median age (IQR), 55 (45-62) years; 53% males] were enrolled in the study.
33942129	2	23	theme	allograft	416:424	arg1	CLAD					439:442	CLAD	439:442	CLAD	439:442	This preliminary study investigated serum galectins as clinical biomarkers in lung transplant patients with chronic lung allograft dysfunction (CLAD), phenotype bronchiolitis obliterans syndrome (BOS).
33942129	2	23	theme	allograft	416:424	arg1	syndrome					481:488	phenotype bronchiolitis obliterans syndrome	446:488	phenotype bronchiolitis obliterans syndrome (BOS)	446:494	This preliminary study investigated serum galectins as clinical biomarkers in lung transplant patients with chronic lung allograft dysfunction (CLAD), phenotype bronchiolitis obliterans syndrome (BOS).
33942129	2	23	theme	allograft	416:424	arg1	dysfunction					426:436	chronic lung allograft dysfunction	403:436	chronic lung allograft dysfunction (CLAD)	403:443	This preliminary study investigated serum galectins as clinical biomarkers in lung transplant patients with chronic lung allograft dysfunction (CLAD), phenotype bronchiolitis obliterans syndrome (BOS).
33942129	11	24	dep	exploring	1628:1636	arg1	worth					1622:1626	worth	1622:1626	worth	1622:1626	The hypothesis that they could be new therapeutic targets in BOS patients is intriguing and also worth exploring.
33942129	7	25	dep	%	1158:1158	arg1	%					1168:1168	NPV 100%	1161:1168	NPV 100%	1161:1168	With the stable LTX group as dependent variable and Gal-1, 3 and 9 as independent variables, AUROC was 92.6% (NPV 100% and PPV 90%, p = 0.0023).
33942129	7	25	dep	%	1158:1158	arg1	%					1180:1180	PPV 90%	1174:1180	PPV 90%	1174:1180	With the stable LTX group as dependent variable and Gal-1, 3 and 9 as independent variables, AUROC was 92.6% (NPV 100% and PPV 90%, p = 0.0023).
33942129	2	26	theme	obliterans	470:479	arg1	BOS					491:493	BOS	491:493	BOS	491:493	This preliminary study investigated serum galectins as clinical biomarkers in lung transplant patients with chronic lung allograft dysfunction (CLAD), phenotype bronchiolitis obliterans syndrome (BOS).
33942129	2	26	theme	obliterans	470:479	arg1	dysfunction					426:436	chronic lung allograft dysfunction	403:436	chronic lung allograft dysfunction (CLAD)	403:443	This preliminary study investigated serum galectins as clinical biomarkers in lung transplant patients with chronic lung allograft dysfunction (CLAD), phenotype bronchiolitis obliterans syndrome (BOS).
33942129	2	26	theme	obliterans	470:479	arg1	syndrome					481:488	phenotype bronchiolitis obliterans syndrome	446:488	phenotype bronchiolitis obliterans syndrome (BOS)	446:494	This preliminary study investigated serum galectins as clinical biomarkers in lung transplant patients with chronic lung allograft dysfunction (CLAD), phenotype bronchiolitis obliterans syndrome (BOS).
33942129	1	27	link	O-linked	172:179	arg1	glycoproteins					181:193	N-linked and O-linked glycoproteins	159:193	N-linked and O-linked glycoproteins	159:193	BACKGROUND Galectins are proteins that bind β-galactosides such as N-acetyllactosamine present in N-linked and O-linked glycoproteins and that seem to be implicated in inflammatory and immune responses as well as fibrotic mechanisms.
33942129	6	28	dep	%	1012:1012	arg1	%					1035:1035	PPV 88.9%	1027:1035	PPV 88.9%	1027:1035	In logistic regression analysis, testing BOS group as dependent variable with Gal-1 and 3 as independent variables, area under the receiver operating characteristics (AUROC) curve was 98.9% (NPV 90% and PPV 88.9%, p = 0.0003).
33942129	6	28	dep	%	1012:1012	arg1	%					1021:1021	NPV 90%	1015:1021	NPV 90%	1015:1021	In logistic regression analysis, testing BOS group as dependent variable with Gal-1 and 3 as independent variables, area under the receiver operating characteristics (AUROC) curve was 98.9% (NPV 90% and PPV 88.9%, p = 0.0003).
33942129	2	29	theme	chronic	403:409	arg1	CLAD					439:442	CLAD	439:442	CLAD	439:442	This preliminary study investigated serum galectins as clinical biomarkers in lung transplant patients with chronic lung allograft dysfunction (CLAD), phenotype bronchiolitis obliterans syndrome (BOS).
33942129	2	29	theme	chronic	403:409	arg1	syndrome					481:488	phenotype bronchiolitis obliterans syndrome	446:488	phenotype bronchiolitis obliterans syndrome (BOS)	446:494	This preliminary study investigated serum galectins as clinical biomarkers in lung transplant patients with chronic lung allograft dysfunction (CLAD), phenotype bronchiolitis obliterans syndrome (BOS).
33942129	2	29	theme	chronic	403:409	arg1	dysfunction					426:436	chronic lung allograft dysfunction	403:436	chronic lung allograft dysfunction (CLAD)	403:443	This preliminary study investigated serum galectins as clinical biomarkers in lung transplant patients with chronic lung allograft dysfunction (CLAD), phenotype bronchiolitis obliterans syndrome (BOS).
33942129	1	30	theme	fibrotic	274:281	arg1	mechanisms					283:292	fibrotic mechanisms	274:292	inflammatory and immune responses as well as fibrotic mechanisms	229:292	BACKGROUND Galectins are proteins that bind β-galactosides such as N-acetyllactosamine present in N-linked and O-linked glycoproteins and that seem to be implicated in inflammatory and immune responses as well as fibrotic mechanisms.
33942129	7	31	theme	PPV	1174:1176	arg1	%					1180:1180	PPV 90%	1174:1180	PPV 90%	1174:1180	With the stable LTX group as dependent variable and Gal-1, 3 and 9 as independent variables, AUROC was 92.6% (NPV 100% and PPV 90%, p = 0.0023).
33942129	2	32	theme	phenotype	446:454	arg1	obliterans					470:479	phenotype bronchiolitis obliterans	446:479	phenotype bronchiolitis obliterans syndrome (BOS)	446:494	This preliminary study investigated serum galectins as clinical biomarkers in lung transplant patients with chronic lung allograft dysfunction (CLAD), phenotype bronchiolitis obliterans syndrome (BOS).
33942129	1	33	link	N-linked	159:166	arg1	glycoproteins					181:193	N-linked and O-linked glycoproteins	159:193	N-linked and O-linked glycoproteins	159:193	BACKGROUND Galectins are proteins that bind β-galactosides such as N-acetyllactosamine present in N-linked and O-linked glycoproteins and that seem to be implicated in inflammatory and immune responses as well as fibrotic mechanisms.
33942129	4	34	theme	ELISA	717:721	arg1	kits					723:726	commercial ELISA kits	706:726	commercial ELISA kits	706:726	Peripheral blood concentrations of galectins-1, 3 and 9 were determined with commercial ELISA kits.
33942129	7	35	theme	LTX	1067:1069	arg1	group					1071:1075	the stable LTX group	1056:1075	the stable LTX group as dependent variable and Gal-1, 3 and 9 as independent variables	1056:1141	With the stable LTX group as dependent variable and Gal-1, 3 and 9 as independent variables, AUROC was 92.6% (NPV 100% and PPV 90%, p = 0.0023).
33942129	6	36	theme	logistic	827:834	arg1	analysis					847:854	logistic regression analysis	827:854	logistic regression analysis	827:854	In logistic regression analysis, testing BOS group as dependent variable with Gal-1 and 3 as independent variables, area under the receiver operating characteristics (AUROC) curve was 98.9% (NPV 90% and PPV 88.9%, p = 0.0003).
33942129	9	37	theme	lung	1378:1381	arg1	patients					1394:1401	lung transplant patients	1378:1401	lung transplant patients with diagnostic and prognostic meaning	1378:1440	CONCLUSION Galectins-1, 3 and 9 are possible clinical biomarkers in lung transplant patients with diagnostic and prognostic meaning.
33942129	6	38	dep	%	1021:1021	arg1	p = 0.0003					1038:1047	p = 0.0003	1038:1047	p = 0.0003	1038:1047	In logistic regression analysis, testing BOS group as dependent variable with Gal-1 and 3 as independent variables, area under the receiver operating characteristics (AUROC) curve was 98.9% (NPV 90% and PPV 88.9%, p = 0.0003).
33942129	6	39	theme	AUROC	991:995	arg1	curve					998:1002	the receiver operating characteristics (AUROC) curve	951:1002	the receiver operating characteristics (AUROC) curve	951:1002	In logistic regression analysis, testing BOS group as dependent variable with Gal-1 and 3 as independent variables, area under the receiver operating characteristics (AUROC) curve was 98.9% (NPV 90% and PPV 88.9%, p = 0.0003).
33942129	10	40	theme	BOS	1520:1522	arg1	mechanisms					1506:1515	the pathological mechanisms	1489:1515	the pathological mechanisms of BOS	1489:1522	These molecules may be directly implicated in the pathological mechanisms of BOS.
33942129	0	41	theme	Lung	33:36	arg1	Dysfunction					48:58	Chronic Lung Allograft Dysfunction	25:58	Chronic Lung Allograft Dysfunction	25:58	The Role of Galectins in Chronic Lung Allograft Dysfunction.
33942129	9	42	theme	diagnostic	1408:1417	arg1	meaning					1434:1440	diagnostic and prognostic meaning	1408:1440	diagnostic and prognostic meaning	1408:1440	CONCLUSION Galectins-1, 3 and 9 are possible clinical biomarkers in lung transplant patients with diagnostic and prognostic meaning.
33942129	8	43	theme	Gal-3	1255:1259	arg1	correlation					1240:1250	an inverse correlation	1229:1250	an inverse correlation of Gal-3 with DLCO% and KCO%, and between Gal-9 and KCO%	1229:1307	In stable patients were observed an inverse correlation of Gal-3 with DLCO% and KCO%, and between Gal-9 and KCO%.
33942129	2	44	with	patients	389:396	arg1	CLAD					439:442	CLAD	439:442	CLAD	439:442	This preliminary study investigated serum galectins as clinical biomarkers in lung transplant patients with chronic lung allograft dysfunction (CLAD), phenotype bronchiolitis obliterans syndrome (BOS).
33942129	2	44	with	patients	389:396	arg1	syndrome					481:488	phenotype bronchiolitis obliterans syndrome	446:488	phenotype bronchiolitis obliterans syndrome (BOS)	446:494	This preliminary study investigated serum galectins as clinical biomarkers in lung transplant patients with chronic lung allograft dysfunction (CLAD), phenotype bronchiolitis obliterans syndrome (BOS).
33942129	2	44	with	patients	389:396	arg1	dysfunction					426:436	chronic lung allograft dysfunction	403:436	chronic lung allograft dysfunction (CLAD)	403:443	This preliminary study investigated serum galectins as clinical biomarkers in lung transplant patients with chronic lung allograft dysfunction (CLAD), phenotype bronchiolitis obliterans syndrome (BOS).
33942129	9	45	theme	prognostic	1423:1432	arg1	meaning					1434:1440	diagnostic and prognostic meaning	1408:1440	diagnostic and prognostic meaning	1408:1440	CONCLUSION Galectins-1, 3 and 9 are possible clinical biomarkers in lung transplant patients with diagnostic and prognostic meaning.
33942129	7	46	theme	NPV	1161:1163	arg1	%					1168:1168	NPV 100%	1161:1168	NPV 100%	1161:1168	With the stable LTX group as dependent variable and Gal-1, 3 and 9 as independent variables, AUROC was 92.6% (NPV 100% and PPV 90%, p = 0.0023).
33942129	8	47	theme	DLCO	1266:1269	arg1	%					1270:1270	DLCO%	1266:1270	DLCO%	1266:1270	In stable patients were observed an inverse correlation of Gal-3 with DLCO% and KCO%, and between Gal-9 and KCO%.
33942129	3	48	theme	transplant	533:542	arg1	patients					544:551	Nineteen lung transplant patients	519:551	Nineteen lung transplant patients	519:551	MATERIALS AND METHODS Nineteen lung transplant patients [median age (IQR), 55 (45-62) years; 53% males] were enrolled in the study.
33942129	6	49	theme	dependent	878:886	arg1	variable					888:895	dependent variable	878:895	dependent variable with Gal-1 and 3	878:912	In logistic regression analysis, testing BOS group as dependent variable with Gal-1 and 3 as independent variables, area under the receiver operating characteristics (AUROC) curve was 98.9% (NPV 90% and PPV 88.9%, p = 0.0003).
33942129	7	50	dep	%	1168:1168	arg1	p = 0.0023					1183:1192	p = 0.0023	1183:1192	p = 0.0023	1183:1192	With the stable LTX group as dependent variable and Gal-1, 3 and 9 as independent variables, AUROC was 92.6% (NPV 100% and PPV 90%, p = 0.0023).
33942129	1	51	gly	glycoproteins	181:193	arg1	glycoproteins					181:193	N-linked and O-linked glycoproteins	159:193	N-linked and O-linked glycoproteins	159:193	BACKGROUND Galectins are proteins that bind β-galactosides such as N-acetyllactosamine present in N-linked and O-linked glycoproteins and that seem to be implicated in inflammatory and immune responses as well as fibrotic mechanisms.
33942129	2	52	theme	lung	373:376	arg1	patients					389:396	lung transplant patients	373:396	lung transplant patients with chronic lung allograft dysfunction (CLAD), phenotype bronchiolitis obliterans syndrome (BOS)	373:494	This preliminary study investigated serum galectins as clinical biomarkers in lung transplant patients with chronic lung allograft dysfunction (CLAD), phenotype bronchiolitis obliterans syndrome (BOS).
33942129	7	53	theme	independent	1121:1131	arg1	variables					1133:1141	independent variables	1121:1141	independent variables	1121:1141	With the stable LTX group as dependent variable and Gal-1, 3 and 9 as independent variables, AUROC was 92.6% (NPV 100% and PPV 90%, p = 0.0023).
33942129	2	54	from	biomarkers	359:368	arg1	patients					389:396	lung transplant patients	373:396	lung transplant patients with chronic lung allograft dysfunction (CLAD), phenotype bronchiolitis obliterans syndrome (BOS)	373:494	This preliminary study investigated serum galectins as clinical biomarkers in lung transplant patients with chronic lung allograft dysfunction (CLAD), phenotype bronchiolitis obliterans syndrome (BOS).
33942129	1	55	attach	present	148:154	arg2	N-acetyllactosamine					128:146	N-acetyllactosamine	128:146	N-acetyllactosamine present in N-linked and O-linked glycoproteins	128:193	BACKGROUND Galectins are proteins that bind β-galactosides such as N-acetyllactosamine present in N-linked and O-linked glycoproteins and that seem to be implicated in inflammatory and immune responses as well as fibrotic mechanisms.
33942129	1	55	attach	present	148:154	arg1	glycoproteins					181:193	N-linked and O-linked glycoproteins	159:193	N-linked and O-linked glycoproteins	159:193	BACKGROUND Galectins are proteins that bind β-galactosides such as N-acetyllactosamine present in N-linked and O-linked glycoproteins and that seem to be implicated in inflammatory and immune responses as well as fibrotic mechanisms.
33942129	3	56	theme	[median	553:559	arg1	years					583:587	55 (45-62) years	572:587	55 (45-62) years	572:587	MATERIALS AND METHODS Nineteen lung transplant patients [median age (IQR), 55 (45-62) years; 53% males] were enrolled in the study.
33942129	3	56	theme	[median	553:559	arg1	IQR					566:568	IQR	566:568	IQR	566:568	MATERIALS AND METHODS Nineteen lung transplant patients [median age (IQR), 55 (45-62) years; 53% males] were enrolled in the study.
33942129	3	56	theme	[median	553:559	arg1	age					561:563	[median age	553:563	[median age (IQR)	553:569	MATERIALS AND METHODS Nineteen lung transplant patients [median age (IQR), 55 (45-62) years; 53% males] were enrolled in the study.
33942129	1	57	theme	O-linked	172:179	arg1	glycoproteins					181:193	N-linked and O-linked glycoproteins	159:193	N-linked and O-linked glycoproteins	159:193	BACKGROUND Galectins are proteins that bind β-galactosides such as N-acetyllactosamine present in N-linked and O-linked glycoproteins and that seem to be implicated in inflammatory and immune responses as well as fibrotic mechanisms.
33942129	2	58	theme	serum	331:335	arg1	galectins					337:345	serum galectins	331:345	serum galectins as clinical biomarkers in lung transplant patients with chronic lung allograft dysfunction (CLAD), phenotype bronchiolitis obliterans syndrome (BOS)	331:494	This preliminary study investigated serum galectins as clinical biomarkers in lung transplant patients with chronic lung allograft dysfunction (CLAD), phenotype bronchiolitis obliterans syndrome (BOS).
33942129	6	59	with	variable	888:895	arg1	Gal-1					902:906	Gal-1	902:906	Gal-1	902:906	In logistic regression analysis, testing BOS group as dependent variable with Gal-1 and 3 as independent variables, area under the receiver operating characteristics (AUROC) curve was 98.9% (NPV 90% and PPV 88.9%, p = 0.0003).
33942129	6	59	with	variable	888:895	arg1	3					912:912	3	912:912	3	912:912	In logistic regression analysis, testing BOS group as dependent variable with Gal-1 and 3 as independent variables, area under the receiver operating characteristics (AUROC) curve was 98.9% (NPV 90% and PPV 88.9%, p = 0.0003).
33942129	4	60	theme	Peripheral	629:638	arg1	concentrations					646:659	Peripheral blood concentrations	629:659	Peripheral blood concentrations of galectins-1, 3 and 9	629:683	Peripheral blood concentrations of galectins-1, 3 and 9 were determined with commercial ELISA kits.
33942129	5	61	theme	LTX	797:799	arg1	p = 0.0394					811:820	p = 0.0394	811:820	p = 0.0394	811:820	RESULTS Galectin-1 concentrations were higher in BOS than in stable LTX patients (p = 0.0394).
33942129	5	61	theme	LTX	797:799	arg1	patients					801:808	stable LTX patients	790:808	stable LTX patients (p = 0.0394)	790:821	RESULTS Galectin-1 concentrations were higher in BOS than in stable LTX patients (p = 0.0394).
33942129	11	62	from	targets	1575:1581	arg1	patients					1590:1597	BOS patients	1586:1597	BOS patients	1586:1597	The hypothesis that they could be new therapeutic targets in BOS patients is intriguing and also worth exploring.
33942129	4	63	theme	3	677:677	arg1	concentrations					646:659	Peripheral blood concentrations	629:659	Peripheral blood concentrations of galectins-1, 3 and 9	629:683	Peripheral blood concentrations of galectins-1, 3 and 9 were determined with commercial ELISA kits.
33942129	4	64	theme	9	683:683	arg1	concentrations					646:659	Peripheral blood concentrations	629:659	Peripheral blood concentrations of galectins-1, 3 and 9	629:683	Peripheral blood concentrations of galectins-1, 3 and 9 were determined with commercial ELISA kits.
33942129	11	65	theme	new	1559:1561	arg1	targets					1575:1581	new therapeutic targets	1559:1581	new therapeutic targets	1559:1581	The hypothesis that they could be new therapeutic targets in BOS patients is intriguing and also worth exploring.
33942129	1	66	theme	present	148:154	arg1	N-acetyllactosamine					128:146	N-acetyllactosamine	128:146	N-acetyllactosamine present in N-linked and O-linked glycoproteins	128:193	BACKGROUND Galectins are proteins that bind β-galactosides such as N-acetyllactosamine present in N-linked and O-linked glycoproteins and that seem to be implicated in inflammatory and immune responses as well as fibrotic mechanisms.
33942129	9	67	from	biomarkers	1364:1373	arg1	patients					1394:1401	lung transplant patients	1378:1401	lung transplant patients with diagnostic and prognostic meaning	1378:1440	CONCLUSION Galectins-1, 3 and 9 are possible clinical biomarkers in lung transplant patients with diagnostic and prognostic meaning.
33942129	6	68	theme	operating	964:972	arg1	curve					998:1002	the receiver operating characteristics (AUROC) curve	951:1002	the receiver operating characteristics (AUROC) curve	951:1002	In logistic regression analysis, testing BOS group as dependent variable with Gal-1 and 3 as independent variables, area under the receiver operating characteristics (AUROC) curve was 98.9% (NPV 90% and PPV 88.9%, p = 0.0003).
33942129	8	69	theme	KCO	1304:1306	arg1	%					1307:1307	KCO%	1304:1307	KCO%	1304:1307	In stable patients were observed an inverse correlation of Gal-3 with DLCO% and KCO%, and between Gal-9 and KCO%.
33942129	5	70	theme	RESULTS	729:735	arg1	concentrations					748:761	RESULTS Galectin-1 concentrations	729:761	RESULTS Galectin-1 concentrations	729:761	RESULTS Galectin-1 concentrations were higher in BOS than in stable LTX patients (p = 0.0394).
33942129	1	71	from	glycoproteins	181:193	arg1	present					148:154	present	148:154	present	148:154	BACKGROUND Galectins are proteins that bind β-galactosides such as N-acetyllactosamine present in N-linked and O-linked glycoproteins and that seem to be implicated in inflammatory and immune responses as well as fibrotic mechanisms.
33942129	1	72	theme	N-linked	159:166	arg1	glycoproteins					181:193	N-linked and O-linked glycoproteins	159:193	N-linked and O-linked glycoproteins	159:193	BACKGROUND Galectins are proteins that bind β-galactosides such as N-acetyllactosamine present in N-linked and O-linked glycoproteins and that seem to be implicated in inflammatory and immune responses as well as fibrotic mechanisms.
33942129	0	73	from	Role	4:7	arg1	Dysfunction					48:58	Chronic Lung Allograft Dysfunction	25:58	Chronic Lung Allograft Dysfunction	25:58	The Role of Galectins in Chronic Lung Allograft Dysfunction.
33942129	4	74	theme	galectins-1	664:674	arg1	concentrations					646:659	Peripheral blood concentrations	629:659	Peripheral blood concentrations of galectins-1, 3 and 9	629:683	Peripheral blood concentrations of galectins-1, 3 and 9 were determined with commercial ELISA kits.
33942129	11	75	theme	BOS	1586:1588	arg1	patients					1590:1597	BOS patients	1586:1597	BOS patients	1586:1597	The hypothesis that they could be new therapeutic targets in BOS patients is intriguing and also worth exploring.
33942129	7	76	theme	dependent	1080:1088	arg1	3					1110:1110	3	1110:1110	3	1110:1110	With the stable LTX group as dependent variable and Gal-1, 3 and 9 as independent variables, AUROC was 92.6% (NPV 100% and PPV 90%, p = 0.0023).
33942129	7	76	theme	dependent	1080:1088	arg1	variable					1090:1097	dependent variable	1080:1097	dependent variable	1080:1097	With the stable LTX group as dependent variable and Gal-1, 3 and 9 as independent variables, AUROC was 92.6% (NPV 100% and PPV 90%, p = 0.0023).
33942129	7	76	theme	dependent	1080:1088	arg1	9					1116:1116	9	1116:1116	9	1116:1116	With the stable LTX group as dependent variable and Gal-1, 3 and 9 as independent variables, AUROC was 92.6% (NPV 100% and PPV 90%, p = 0.0023).
33942129	1	77	theme	BACKGROUND	61:70	arg1	proteins					86:93	proteins	86:93	proteins that bind β-galactosides such as N-acetyllactosamine present in N-linked and O-linked glycoproteins and that seem to be implicated in inflammatory and immune responses as well as fibrotic mechanisms	86:292	BACKGROUND Galectins are proteins that bind β-galactosides such as N-acetyllactosamine present in N-linked and O-linked glycoproteins and that seem to be implicated in inflammatory and immune responses as well as fibrotic mechanisms.
33942129	1	77	theme	BACKGROUND	61:70	arg1	Galectins					72:80	BACKGROUND Galectins	61:80	BACKGROUND Galectins	61:80	BACKGROUND Galectins are proteins that bind β-galactosides such as N-acetyllactosamine present in N-linked and O-linked glycoproteins and that seem to be implicated in inflammatory and immune responses as well as fibrotic mechanisms.
33942129	6	78	theme	NPV	1015:1017	arg1	%					1021:1021	NPV 90%	1015:1021	NPV 90%	1015:1021	In logistic regression analysis, testing BOS group as dependent variable with Gal-1 and 3 as independent variables, area under the receiver operating characteristics (AUROC) curve was 98.9% (NPV 90% and PPV 88.9%, p = 0.0003).
33942129	2	79	theme	lung	411:414	arg1	CLAD					439:442	CLAD	439:442	CLAD	439:442	This preliminary study investigated serum galectins as clinical biomarkers in lung transplant patients with chronic lung allograft dysfunction (CLAD), phenotype bronchiolitis obliterans syndrome (BOS).
33942129	2	79	theme	lung	411:414	arg1	syndrome					481:488	phenotype bronchiolitis obliterans syndrome	446:488	phenotype bronchiolitis obliterans syndrome (BOS)	446:494	This preliminary study investigated serum galectins as clinical biomarkers in lung transplant patients with chronic lung allograft dysfunction (CLAD), phenotype bronchiolitis obliterans syndrome (BOS).
33942129	2	79	theme	lung	411:414	arg1	dysfunction					426:436	chronic lung allograft dysfunction	403:436	chronic lung allograft dysfunction (CLAD)	403:443	This preliminary study investigated serum galectins as clinical biomarkers in lung transplant patients with chronic lung allograft dysfunction (CLAD), phenotype bronchiolitis obliterans syndrome (BOS).
33942129	9	80	theme	clinical	1355:1362	arg1	biomarkers					1364:1373	possible clinical biomarkers	1346:1373	possible clinical biomarkers in lung transplant patients with diagnostic and prognostic meaning	1346:1440	CONCLUSION Galectins-1, 3 and 9 are possible clinical biomarkers in lung transplant patients with diagnostic and prognostic meaning.
33942129	9	80	theme	clinical	1355:1362	arg1	CONCLUSION					1310:1319	CONCLUSION Galectins-1, 3 and 9	1310:1340	CONCLUSION Galectins-1, 3 and 9	1310:1340	CONCLUSION Galectins-1, 3 and 9 are possible clinical biomarkers in lung transplant patients with diagnostic and prognostic meaning.
33942129	2	81	theme	bronchiolitis	456:468	arg1	obliterans					470:479	phenotype bronchiolitis obliterans	446:479	phenotype bronchiolitis obliterans syndrome (BOS)	446:494	This preliminary study investigated serum galectins as clinical biomarkers in lung transplant patients with chronic lung allograft dysfunction (CLAD), phenotype bronchiolitis obliterans syndrome (BOS).
33942129	0	82	theme	Galectins	12:20	arg1	Role					4:7	The Role	0:7	The Role of Galectins in Chronic Lung Allograft Dysfunction	0:58	The Role of Galectins in Chronic Lung Allograft Dysfunction.
33942129	4	83	theme	commercial	706:715	arg1	kits					723:726	commercial ELISA kits	706:726	commercial ELISA kits	706:726	Peripheral blood concentrations of galectins-1, 3 and 9 were determined with commercial ELISA kits.
33942129	0	84	theme	Chronic	25:31	arg1	Dysfunction					48:58	Chronic Lung Allograft Dysfunction	25:58	Chronic Lung Allograft Dysfunction	25:58	The Role of Galectins in Chronic Lung Allograft Dysfunction.
33942129	9	85	theme	transplant	1383:1392	arg1	patients					1394:1401	lung transplant patients	1378:1401	lung transplant patients with diagnostic and prognostic meaning	1378:1440	CONCLUSION Galectins-1, 3 and 9 are possible clinical biomarkers in lung transplant patients with diagnostic and prognostic meaning.
33942129	0	86	theme	Allograft	38:46	arg1	Dysfunction					48:58	Chronic Lung Allograft Dysfunction	25:58	Chronic Lung Allograft Dysfunction	25:58	The Role of Galectins in Chronic Lung Allograft Dysfunction.
33942129	8	87	theme	inverse	1232:1238	arg1	correlation					1240:1250	an inverse correlation	1229:1250	an inverse correlation of Gal-3 with DLCO% and KCO%, and between Gal-9 and KCO%	1229:1307	In stable patients were observed an inverse correlation of Gal-3 with DLCO% and KCO%, and between Gal-9 and KCO%.
33942129	2	88	theme	clinical	350:357	arg1	biomarkers					359:368	clinical biomarkers	350:368	clinical biomarkers in lung transplant patients with chronic lung allograft dysfunction (CLAD), phenotype bronchiolitis obliterans syndrome (BOS)	350:494	This preliminary study investigated serum galectins as clinical biomarkers in lung transplant patients with chronic lung allograft dysfunction (CLAD), phenotype bronchiolitis obliterans syndrome (BOS).
33942129	9	89	theme	possible	1346:1353	arg1	biomarkers					1364:1373	possible clinical biomarkers	1346:1373	possible clinical biomarkers in lung transplant patients with diagnostic and prognostic meaning	1346:1440	CONCLUSION Galectins-1, 3 and 9 are possible clinical biomarkers in lung transplant patients with diagnostic and prognostic meaning.
33942129	9	89	theme	possible	1346:1353	arg1	CONCLUSION					1310:1319	CONCLUSION Galectins-1, 3 and 9	1310:1340	CONCLUSION Galectins-1, 3 and 9	1310:1340	CONCLUSION Galectins-1, 3 and 9 are possible clinical biomarkers in lung transplant patients with diagnostic and prognostic meaning.
33942129	10	90	theme	pathological	1493:1504	arg1	mechanisms					1506:1515	the pathological mechanisms	1489:1515	the pathological mechanisms of BOS	1489:1522	These molecules may be directly implicated in the pathological mechanisms of BOS.
33942129	6	91	theme	PPV	1027:1029	arg1	%					1035:1035	PPV 88.9%	1027:1035	PPV 88.9%	1027:1035	In logistic regression analysis, testing BOS group as dependent variable with Gal-1 and 3 as independent variables, area under the receiver operating characteristics (AUROC) curve was 98.9% (NPV 90% and PPV 88.9%, p = 0.0003).
33942129	2	92	theme	transplant	378:387	arg1	patients					389:396	lung transplant patients	373:396	lung transplant patients with chronic lung allograft dysfunction (CLAD), phenotype bronchiolitis obliterans syndrome (BOS)	373:494	This preliminary study investigated serum galectins as clinical biomarkers in lung transplant patients with chronic lung allograft dysfunction (CLAD), phenotype bronchiolitis obliterans syndrome (BOS).
33942129	1	93	theme	immune	246:251	arg1	responses					253:261	immune responses	246:261	immune responses	246:261	BACKGROUND Galectins are proteins that bind β-galactosides such as N-acetyllactosamine present in N-linked and O-linked glycoproteins and that seem to be implicated in inflammatory and immune responses as well as fibrotic mechanisms.
33807002	11	0	theme	laboratory	1824:1833	arg1	markers					1835:1841	possible CDG-ALG13 laboratory markers	1805:1841	possible CDG-ALG13 laboratory markers that would have clinical significance	1805:1879	Our intention was to turn attention toward possible CDG-ALG13 laboratory markers that would have clinical significance.
33807002	1	1	dep	oligosaccharides	282:297	arg1	pathways					374:381	pathways	374:381	pathways	374:381	BACKGROUND ALG13-CDG belongs to the congenital disorders of glycosylation (CDG), which is an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity.
33807002	11	2	theme	CDG-ALG13	1814:1822	arg1	markers					1835:1841	possible CDG-ALG13 laboratory markers	1805:1841	possible CDG-ALG13 laboratory markers that would have clinical significance	1805:1879	Our intention was to turn attention toward possible CDG-ALG13 laboratory markers that would have clinical significance.
33807002	1	3	theme	disorders	239:247	arg1	disorders					150:158	the congenital disorders	135:158	the congenital disorders	135:158	BACKGROUND ALG13-CDG belongs to the congenital disorders of glycosylation (CDG), which is an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity.
33807002	1	3	theme	disorders	239:247	arg1	group					206:210	an expanding group	193:210	an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity	193:413	BACKGROUND ALG13-CDG belongs to the congenital disorders of glycosylation (CDG), which is an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity.
33807002	1	3	theme	disorders	239:247	arg1	disorders					239:247	multisystemic metabolic disorders	215:247	multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity	215:413	BACKGROUND ALG13-CDG belongs to the congenital disorders of glycosylation (CDG), which is an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity.
33807002	4	4	with	patients	704:711	arg1	encephalopathy					728:741	epileptic encephalopathy	718:741	epileptic encephalopathy due to the recurrent c.320A>G variant in ALG13	718:788	This manuscript describes the first metabolomic analysis using NMR in three patients with epileptic encephalopathy due to the recurrent c.320A>G variant in ALG13, characterized to date only in about 60 individuals (mostly female).
33807002	1	5	theme	GPI	346:348	arg1	anchors					351:357	glycophosphatidylinositol (GPI) anchors	319:357	glycophosphatidylinositol (GPI) anchors	319:357	BACKGROUND ALG13-CDG belongs to the congenital disorders of glycosylation (CDG), which is an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity.
33807002	1	6	with	substrates	307:316	arg1	heterogeneity					401:413	high genetic heterogeneity	388:413	high genetic heterogeneity	388:413	BACKGROUND ALG13-CDG belongs to the congenital disorders of glycosylation (CDG), which is an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity.
33807002	1	7	theme	glycosylation	163:175	arg1	disorders					150:158	the congenital disorders	135:158	the congenital disorders	135:158	BACKGROUND ALG13-CDG belongs to the congenital disorders of glycosylation (CDG), which is an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity.
33807002	1	7	theme	glycosylation	163:175	arg1	group					206:210	an expanding group	193:210	an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity	193:413	BACKGROUND ALG13-CDG belongs to the congenital disorders of glycosylation (CDG), which is an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity.
33807002	1	7	theme	glycosylation	163:175	arg1	disorders					239:247	multisystemic metabolic disorders	215:247	multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity	215:413	BACKGROUND ALG13-CDG belongs to the congenital disorders of glycosylation (CDG), which is an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity.
33807002	1	8	link	N-linked	263:270	arg1	oligosaccharides					282:297	the N-linked, O-linked oligosaccharides	259:297	the N-linked, O-linked oligosaccharides	259:297	BACKGROUND ALG13-CDG belongs to the congenital disorders of glycosylation (CDG), which is an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity.
33807002	8	9	theme	metabolic	1454:1462	arg1	profile					1464:1470	the metabolic profile	1450:1470	the metabolic profile of the blood serum samples acquired from the studied patients	1450:1532	METHODS Nuclear magnetic resonance (NMR) spectroscopy in conjunction with multivariate and univariate modelling were used to analyze the metabolic profile of the blood serum samples acquired from the studied patients.
33807002	10	10	theme	further	1745:1751	arg1	studies					1753:1759	further studies	1745:1759	further studies	1745:1759	CONCLUSIONS Since presented data are the first to be collected so far, they need be verified in further studies.
33807002	4	11	theme	recurrent	754:762	arg1	variant					773:779	the recurrent c.320A>G variant	750:779	the recurrent c.320A>G variant in ALG13	750:788	This manuscript describes the first metabolomic analysis using NMR in three patients with epileptic encephalopathy due to the recurrent c.320A>G variant in ALG13, characterized to date only in about 60 individuals (mostly female).
33807002	5	12	theme	pathogenesis	925:936	arg1	understanding					904:916	the understanding	900:916	the understanding of the pathogenesis of the disease associated with this variant in the rare genetic condition	900:1010	This is an important preliminary step in the understanding of the pathogenesis of the disease associated with this variant in the rare genetic condition.
33807002	2	13	theme	many	505:508	arg1	questions					510:518	many questions	505:518	many questions	505:518	Thus, as far as clinical presentation, laboratory findings, and treatment are concerned, many questions are to be answered.
33807002	9	14	theme	RESULTS	1535:1541	arg1	biomarkers					1590:1599	potential biomarkers	1580:1599	potential biomarkers: betaine, N-acetyl-glycoprotein, and carnitine	1580:1646	RESULTS Three metabolites were identified as potential biomarkers: betaine, N-acetyl-glycoprotein, and carnitine.
33807002	9	14	theme	RESULTS	1535:1541	arg1	metabolites					1549:1559	RESULTS Three metabolites	1535:1559	RESULTS Three metabolites	1535:1559	RESULTS Three metabolites were identified as potential biomarkers: betaine, N-acetyl-glycoprotein, and carnitine.
33807002	6	15	theme	protein	1128:1134	arg1	function					1106:1113	the only known function	1091:1113	the only known function of the ALG13 protein	1091:1134	The disease is assumed to be a disorder of N-glycosylation given that this is the only known function of the ALG13 protein.
33807002	6	15	theme	protein	1128:1134	arg1	this					1083:1086	this	1083:1086	this	1083:1086	The disease is assumed to be a disorder of N-glycosylation given that this is the only known function of the ALG13 protein.
33807002	5	16	from	variant	974:980	arg1	condition					1002:1010	the rare genetic condition	985:1010	the rare genetic condition	985:1010	This is an important preliminary step in the understanding of the pathogenesis of the disease associated with this variant in the rare genetic condition.
33807002	7	17	from	abnormal	1285:1292	arg1	patients					1307:1314	the ALG13 patients	1297:1314	the ALG13 patients	1297:1314	Despite this, protein electrophoresis, which is abnormal in most conditions due to abnormalities in N-glycosylation, has been normal or only mildly abnormal in the ALG13 patients.
33807002	11	18	contain	have	1854:1857	arg2	significance					1868:1879	clinical significance	1859:1879	clinical significance	1859:1879	Our intention was to turn attention toward possible CDG-ALG13 laboratory markers that would have clinical significance.
33807002	11	18	contain	have	1854:1857	arg1	markers					1835:1841	possible CDG-ALG13 laboratory markers	1805:1841	possible CDG-ALG13 laboratory markers that would have clinical significance	1805:1879	Our intention was to turn attention toward possible CDG-ALG13 laboratory markers that would have clinical significance.
33807002	7	19	from	conditions	1202:1211	arg1	abnormal					1185:1192	abnormal	1185:1192	abnormal	1185:1192	Despite this, protein electrophoresis, which is abnormal in most conditions due to abnormalities in N-glycosylation, has been normal or only mildly abnormal in the ALG13 patients.
33807002	5	20	theme	disease	945:951	arg1	pathogenesis					925:936	the pathogenesis	921:936	the pathogenesis of the disease associated with this variant in the rare genetic condition	921:1010	This is an important preliminary step in the understanding of the pathogenesis of the disease associated with this variant in the rare genetic condition.
33807002	11	21	theme	clinical	1859:1866	arg1	significance					1868:1879	clinical significance	1859:1879	clinical significance	1859:1879	Our intention was to turn attention toward possible CDG-ALG13 laboratory markers that would have clinical significance.
33807002	5	22	theme	important	870:878	arg1	step					892:895	an important preliminary step	867:895	an important preliminary step in the understanding of the pathogenesis of the disease associated with this variant in the rare genetic condition	867:1010	This is an important preliminary step in the understanding of the pathogenesis of the disease associated with this variant in the rare genetic condition.
33807002	5	22	theme	important	870:878	arg1	This					859:862	This	859:862	This	859:862	This is an important preliminary step in the understanding of the pathogenesis of the disease associated with this variant in the rare genetic condition.
33807002	4	23	theme	c.320A>G	764:771	arg1	variant					773:779	the recurrent c.320A>G variant	750:779	the recurrent c.320A>G variant in ALG13	750:788	This manuscript describes the first metabolomic analysis using NMR in three patients with epileptic encephalopathy due to the recurrent c.320A>G variant in ALG13, characterized to date only in about 60 individuals (mostly female).
33807002	8	24	dep	METHODS	1317:1323	arg1	spectroscopy					1358:1369	Nuclear magnetic resonance (NMR) spectroscopy	1325:1369	METHODS Nuclear magnetic resonance (NMR) spectroscopy in conjunction with multivariate and univariate modelling	1317:1427	METHODS Nuclear magnetic resonance (NMR) spectroscopy in conjunction with multivariate and univariate modelling were used to analyze the metabolic profile of the blood serum samples acquired from the studied patients.
33807002	0	25	theme	First	4:8	arg1	Analysis					21:28	The First Metabolome Analysis	0:28	The First Metabolome Analysis in Children with Epilepsy and ALG13-CDG	0:68	The First Metabolome Analysis in Children with Epilepsy and ALG13-CDG Resulting from c.320A>G Variant.
33807002	8	26	theme	blood	1479:1483	arg1	samples					1491:1497	the blood serum samples	1475:1497	the blood serum samples acquired from the studied patients	1475:1532	METHODS Nuclear magnetic resonance (NMR) spectroscopy in conjunction with multivariate and univariate modelling were used to analyze the metabolic profile of the blood serum samples acquired from the studied patients.
33807002	1	27	with	oligosaccharides	282:297	arg1	heterogeneity					401:413	high genetic heterogeneity	388:413	high genetic heterogeneity	388:413	BACKGROUND ALG13-CDG belongs to the congenital disorders of glycosylation (CDG), which is an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity.
33807002	4	28	theme	first	658:662	arg1	analysis					676:683	the first metabolomic analysis	654:683	the first metabolomic analysis using NMR in three patients with epileptic encephalopathy due to the recurrent c.320A>G variant in ALG13, characterized to date	654:811	This manuscript describes the first metabolomic analysis using NMR in three patients with epileptic encephalopathy due to the recurrent c.320A>G variant in ALG13, characterized to date only in about 60 individuals (mostly female).
33807002	1	29	dep	N-linked	263:270	arg1	O-linked					273:280	O-linked	273:280	O-linked	273:280	BACKGROUND ALG13-CDG belongs to the congenital disorders of glycosylation (CDG), which is an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity.
33807002	1	30	theme	N-linked	263:270	arg1	oligosaccharides					282:297	the N-linked, O-linked oligosaccharides	259:297	the N-linked, O-linked oligosaccharides	259:297	BACKGROUND ALG13-CDG belongs to the congenital disorders of glycosylation (CDG), which is an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity.
33807002	7	31	from	abnormalities	1220:1232	arg1	N-glycosylation					1237:1251	N-glycosylation	1237:1251	N-glycosylation	1237:1251	Despite this, protein electrophoresis, which is abnormal in most conditions due to abnormalities in N-glycosylation, has been normal or only mildly abnormal in the ALG13 patients.
33807002	4	32	theme	epileptic	718:726	arg1	encephalopathy					728:741	epileptic encephalopathy	718:741	epileptic encephalopathy due to the recurrent c.320A>G variant in ALG13	718:788	This manuscript describes the first metabolomic analysis using NMR in three patients with epileptic encephalopathy due to the recurrent c.320A>G variant in ALG13, characterized to date only in about 60 individuals (mostly female).
33807002	7	33	from	patients	1307:1314	arg1	abnormal					1285:1292	abnormal	1285:1292	abnormal	1285:1292	Despite this, protein electrophoresis, which is abnormal in most conditions due to abnormalities in N-glycosylation, has been normal or only mildly abnormal in the ALG13 patients.
33807002	8	34	theme	samples	1491:1497	arg1	profile					1464:1470	the metabolic profile	1450:1470	the metabolic profile of the blood serum samples acquired from the studied patients	1450:1532	METHODS Nuclear magnetic resonance (NMR) spectroscopy in conjunction with multivariate and univariate modelling were used to analyze the metabolic profile of the blood serum samples acquired from the studied patients.
33807002	5	35	from	step	892:895	arg1	understanding					904:916	the understanding	900:916	the understanding of the pathogenesis of the disease associated with this variant in the rare genetic condition	900:1010	This is an important preliminary step in the understanding of the pathogenesis of the disease associated with this variant in the rare genetic condition.
33807002	6	36	theme	known	1100:1104	arg1	function					1106:1113	the only known function	1091:1113	the only known function of the ALG13 protein	1091:1134	The disease is assumed to be a disorder of N-glycosylation given that this is the only known function of the ALG13 protein.
33807002	6	36	theme	known	1100:1104	arg1	this					1083:1086	this	1083:1086	this	1083:1086	The disease is assumed to be a disorder of N-glycosylation given that this is the only known function of the ALG13 protein.
33807002	4	37	from	variant	773:779	arg1	ALG13					784:788	ALG13	784:788	ALG13	784:788	This manuscript describes the first metabolomic analysis using NMR in three patients with epileptic encephalopathy due to the recurrent c.320A>G variant in ALG13, characterized to date only in about 60 individuals (mostly female).
33807002	7	38	from	abnormal	1185:1192	arg1	conditions					1202:1211	most conditions	1197:1211	most conditions	1197:1211	Despite this, protein electrophoresis, which is abnormal in most conditions due to abnormalities in N-glycosylation, has been normal or only mildly abnormal in the ALG13 patients.
33807002	0	39	theme	Metabolome	10:19	arg1	Analysis					21:28	The First Metabolome Analysis	0:28	The First Metabolome Analysis in Children with Epilepsy and ALG13-CDG	0:68	The First Metabolome Analysis in Children with Epilepsy and ALG13-CDG Resulting from c.320A>G Variant.
33807002	6	40	theme	ALG13	1122:1126	arg1	protein					1128:1134	the ALG13 protein	1118:1134	the ALG13 protein	1118:1134	The disease is assumed to be a disorder of N-glycosylation given that this is the only known function of the ALG13 protein.
33807002	8	41	theme	multivariate	1391:1402	arg1	modelling					1419:1427	multivariate and univariate modelling	1391:1427	multivariate and univariate modelling	1391:1427	METHODS Nuclear magnetic resonance (NMR) spectroscopy in conjunction with multivariate and univariate modelling were used to analyze the metabolic profile of the blood serum samples acquired from the studied patients.
33807002	1	42	theme	BACKGROUND	103:112	arg1	ALG13-CDG					114:122	BACKGROUND ALG13-CDG	103:122	BACKGROUND ALG13-CDG	103:122	BACKGROUND ALG13-CDG belongs to the congenital disorders of glycosylation (CDG), which is an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity.
33807002	8	43	from	spectroscopy	1358:1369	arg1	conjunction					1374:1384	conjunction	1374:1384	conjunction with multivariate and univariate modelling	1374:1427	METHODS Nuclear magnetic resonance (NMR) spectroscopy in conjunction with multivariate and univariate modelling were used to analyze the metabolic profile of the blood serum samples acquired from the studied patients.
33807002	7	44	theme	most	1197:1200	arg1	conditions					1202:1211	most conditions	1197:1211	most conditions	1197:1211	Despite this, protein electrophoresis, which is abnormal in most conditions due to abnormalities in N-glycosylation, has been normal or only mildly abnormal in the ALG13 patients.
33807002	11	45	theme	possible	1805:1812	arg1	markers					1835:1841	possible CDG-ALG13 laboratory markers	1805:1841	possible CDG-ALG13 laboratory markers that would have clinical significance	1805:1879	Our intention was to turn attention toward possible CDG-ALG13 laboratory markers that would have clinical significance.
33807002	8	46	theme	resonance	1342:1350	arg1	spectroscopy					1358:1369	Nuclear magnetic resonance (NMR) spectroscopy	1325:1369	METHODS Nuclear magnetic resonance (NMR) spectroscopy in conjunction with multivariate and univariate modelling	1317:1427	METHODS Nuclear magnetic resonance (NMR) spectroscopy in conjunction with multivariate and univariate modelling were used to analyze the metabolic profile of the blood serum samples acquired from the studied patients.
33807002	9	47	gly	N-acetyl-glycoprotein	1611:1631	arg1	N-acetyl-glycoprotein					1611:1631	N-acetyl-glycoprotein	1611:1631	N-acetyl-glycoprotein	1611:1631	RESULTS Three metabolites were identified as potential biomarkers: betaine, N-acetyl-glycoprotein, and carnitine.
33807002	9	47	gly	N-acetyl-glycoprotein	1611:1631	arg1	biomarkers					1590:1599	potential biomarkers	1580:1599	potential biomarkers: betaine, N-acetyl-glycoprotein, and carnitine	1580:1646	RESULTS Three metabolites were identified as potential biomarkers: betaine, N-acetyl-glycoprotein, and carnitine.
33807002	8	48	with	conjunction	1374:1384	arg1	modelling					1419:1427	multivariate and univariate modelling	1391:1427	multivariate and univariate modelling	1391:1427	METHODS Nuclear magnetic resonance (NMR) spectroscopy in conjunction with multivariate and univariate modelling were used to analyze the metabolic profile of the blood serum samples acquired from the studied patients.
33807002	2	49	theme	laboratory	455:464	arg1	findings					466:473	laboratory findings	455:473	laboratory findings	455:473	Thus, as far as clinical presentation, laboratory findings, and treatment are concerned, many questions are to be answered.
33807002	8	50	used	used	1434:1437	arg2	METHODS					1317:1323	METHODS	1317:1323	METHODS Nuclear magnetic resonance (NMR) spectroscopy in conjunction with multivariate and univariate modelling	1317:1427	METHODS Nuclear magnetic resonance (NMR) spectroscopy in conjunction with multivariate and univariate modelling were used to analyze the metabolic profile of the blood serum samples acquired from the studied patients.
33807002	8	51	theme	studied	1517:1523	arg1	patients					1525:1532	the studied patients	1513:1532	the studied patients	1513:1532	METHODS Nuclear magnetic resonance (NMR) spectroscopy in conjunction with multivariate and univariate modelling were used to analyze the metabolic profile of the blood serum samples acquired from the studied patients.
33807002	1	52	theme	high	388:391	arg1	heterogeneity					401:413	high genetic heterogeneity	388:413	high genetic heterogeneity	388:413	BACKGROUND ALG13-CDG belongs to the congenital disorders of glycosylation (CDG), which is an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity.
33807002	5	53	theme	rare	989:992	arg1	condition					1002:1010	the rare genetic condition	985:1010	the rare genetic condition	985:1010	This is an important preliminary step in the understanding of the pathogenesis of the disease associated with this variant in the rare genetic condition.
33807002	1	54	theme	expanding	196:204	arg1	disorders					150:158	the congenital disorders	135:158	the congenital disorders	135:158	BACKGROUND ALG13-CDG belongs to the congenital disorders of glycosylation (CDG), which is an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity.
33807002	1	54	theme	expanding	196:204	arg1	group					206:210	an expanding group	193:210	an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity	193:413	BACKGROUND ALG13-CDG belongs to the congenital disorders of glycosylation (CDG), which is an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity.
33807002	1	54	theme	expanding	196:204	arg1	disorders					239:247	multisystemic metabolic disorders	215:247	multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity	215:413	BACKGROUND ALG13-CDG belongs to the congenital disorders of glycosylation (CDG), which is an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity.
33807002	1	55	theme	shared	300:305	arg1	substrates					307:316	shared substrates	300:316	shared substrates	300:316	BACKGROUND ALG13-CDG belongs to the congenital disorders of glycosylation (CDG), which is an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity.
33807002	1	56	theme	genetic	393:399	arg1	heterogeneity					401:413	high genetic heterogeneity	388:413	high genetic heterogeneity	388:413	BACKGROUND ALG13-CDG belongs to the congenital disorders of glycosylation (CDG), which is an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity.
33807002	5	57	theme	genetic	994:1000	arg1	condition					1002:1010	the rare genetic condition	985:1010	the rare genetic condition	985:1010	This is an important preliminary step in the understanding of the pathogenesis of the disease associated with this variant in the rare genetic condition.
33807002	8	58	theme	serum	1485:1489	arg1	samples					1491:1497	the blood serum samples	1475:1497	the blood serum samples acquired from the studied patients	1475:1532	METHODS Nuclear magnetic resonance (NMR) spectroscopy in conjunction with multivariate and univariate modelling were used to analyze the metabolic profile of the blood serum samples acquired from the studied patients.
33807002	5	59	theme	preliminary	880:890	arg1	step					892:895	an important preliminary step	867:895	an important preliminary step in the understanding of the pathogenesis of the disease associated with this variant in the rare genetic condition	867:1010	This is an important preliminary step in the understanding of the pathogenesis of the disease associated with this variant in the rare genetic condition.
33807002	5	59	theme	preliminary	880:890	arg1	This					859:862	This	859:862	This	859:862	This is an important preliminary step in the understanding of the pathogenesis of the disease associated with this variant in the rare genetic condition.
33807002	1	60	theme	glycophosphatidylinositol	319:343	arg1	anchors					351:357	glycophosphatidylinositol (GPI) anchors	319:357	glycophosphatidylinositol (GPI) anchors	319:357	BACKGROUND ALG13-CDG belongs to the congenital disorders of glycosylation (CDG), which is an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity.
33807002	3	61	theme	heterogeneity	613:625	arg1	individuals					546:556	Three individuals	540:556	Three individuals presented here	540:571	Three individuals presented here may serve as a good example of clinical heterogeneity.
33807002	3	61	theme	heterogeneity	613:625	arg1	example					593:599	a good example	586:599	a good example of clinical heterogeneity	586:625	Three individuals presented here may serve as a good example of clinical heterogeneity.
33807002	7	62	theme	protein	1151:1157	arg1	electrophoresis					1159:1173	protein electrophoresis	1151:1173	protein electrophoresis	1151:1173	Despite this, protein electrophoresis, which is abnormal in most conditions due to abnormalities in N-glycosylation, has been normal or only mildly abnormal in the ALG13 patients.
33807002	3	63	theme	clinical	604:611	arg1	heterogeneity					613:625	clinical heterogeneity	604:625	clinical heterogeneity	604:625	Three individuals presented here may serve as a good example of clinical heterogeneity.
33807002	8	64	theme	magnetic	1333:1340	arg1	NMR					1353:1355	NMR	1353:1355	NMR	1353:1355	METHODS Nuclear magnetic resonance (NMR) spectroscopy in conjunction with multivariate and univariate modelling were used to analyze the metabolic profile of the blood serum samples acquired from the studied patients.
33807002	8	64	theme	magnetic	1333:1340	arg1	resonance					1342:1350	Nuclear magnetic resonance	1325:1350	METHODS Nuclear magnetic resonance (NMR) spectroscopy in conjunction with multivariate and univariate modelling	1317:1427	METHODS Nuclear magnetic resonance (NMR) spectroscopy in conjunction with multivariate and univariate modelling were used to analyze the metabolic profile of the blood serum samples acquired from the studied patients.
33807002	10	65	theme	presented	1667:1675	arg1	data					1677:1680	presented data	1667:1680	presented data	1667:1680	CONCLUSIONS Since presented data are the first to be collected so far, they need be verified in further studies.
33807002	1	66	with	dolichols	364:372	arg1	heterogeneity					401:413	high genetic heterogeneity	388:413	high genetic heterogeneity	388:413	BACKGROUND ALG13-CDG belongs to the congenital disorders of glycosylation (CDG), which is an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity.
33807002	9	67	theme	potential	1580:1588	arg1	N-acetyl-glycoprotein					1611:1631	N-acetyl-glycoprotein	1611:1631	N-acetyl-glycoprotein	1611:1631	RESULTS Three metabolites were identified as potential biomarkers: betaine, N-acetyl-glycoprotein, and carnitine.
33807002	9	67	theme	potential	1580:1588	arg1	carnitine					1638:1646	carnitine	1638:1646	carnitine	1638:1646	RESULTS Three metabolites were identified as potential biomarkers: betaine, N-acetyl-glycoprotein, and carnitine.
33807002	9	67	theme	potential	1580:1588	arg1	betaine					1602:1608	betaine	1602:1608	betaine	1602:1608	RESULTS Three metabolites were identified as potential biomarkers: betaine, N-acetyl-glycoprotein, and carnitine.
33807002	9	67	theme	potential	1580:1588	arg1	biomarkers					1590:1599	potential biomarkers	1580:1599	potential biomarkers: betaine, N-acetyl-glycoprotein, and carnitine	1580:1646	RESULTS Three metabolites were identified as potential biomarkers: betaine, N-acetyl-glycoprotein, and carnitine.
33807002	9	67	theme	potential	1580:1588	arg1	metabolites					1549:1559	RESULTS Three metabolites	1535:1559	RESULTS Three metabolites	1535:1559	RESULTS Three metabolites were identified as potential biomarkers: betaine, N-acetyl-glycoprotein, and carnitine.
33807002	4	68	theme	metabolomic	664:674	arg1	analysis					676:683	the first metabolomic analysis	654:683	the first metabolomic analysis using NMR in three patients with epileptic encephalopathy due to the recurrent c.320A>G variant in ALG13, characterized to date	654:811	This manuscript describes the first metabolomic analysis using NMR in three patients with epileptic encephalopathy due to the recurrent c.320A>G variant in ALG13, characterized to date only in about 60 individuals (mostly female).
33807002	1	69	with	anchors	351:357	arg1	heterogeneity					401:413	high genetic heterogeneity	388:413	high genetic heterogeneity	388:413	BACKGROUND ALG13-CDG belongs to the congenital disorders of glycosylation (CDG), which is an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity.
33807002	11	70	theme	turn	1783:1786	arg1	attention					1788:1796	turn attention	1783:1796	turn attention toward possible CDG-ALG13 laboratory markers that would have clinical significance	1783:1879	Our intention was to turn attention toward possible CDG-ALG13 laboratory markers that would have clinical significance.
33807002	8	71	theme	univariate	1408:1417	arg1	modelling					1419:1427	multivariate and univariate modelling	1391:1427	multivariate and univariate modelling	1391:1427	METHODS Nuclear magnetic resonance (NMR) spectroscopy in conjunction with multivariate and univariate modelling were used to analyze the metabolic profile of the blood serum samples acquired from the studied patients.
33807002	6	72	theme	N-glycosylation	1056:1070	arg1	disease					1017:1023	The disease	1013:1023	The disease	1013:1023	The disease is assumed to be a disorder of N-glycosylation given that this is the only known function of the ALG13 protein.
33807002	6	72	theme	N-glycosylation	1056:1070	arg1	disorder					1044:1051	a disorder	1042:1051	a disorder of N-glycosylation given that this is the only known function of the ALG13 protein	1042:1134	The disease is assumed to be a disorder of N-glycosylation given that this is the only known function of the ALG13 protein.
33807002	9	73	dep	biomarkers	1590:1599	arg1	N-acetyl-glycoprotein					1611:1631	N-acetyl-glycoprotein	1611:1631	N-acetyl-glycoprotein	1611:1631	RESULTS Three metabolites were identified as potential biomarkers: betaine, N-acetyl-glycoprotein, and carnitine.
33807002	9	73	dep	biomarkers	1590:1599	arg1	carnitine					1638:1646	carnitine	1638:1646	carnitine	1638:1646	RESULTS Three metabolites were identified as potential biomarkers: betaine, N-acetyl-glycoprotein, and carnitine.
33807002	9	73	dep	biomarkers	1590:1599	arg1	betaine					1602:1608	betaine	1602:1608	betaine	1602:1608	RESULTS Three metabolites were identified as potential biomarkers: betaine, N-acetyl-glycoprotein, and carnitine.
33807002	9	73	dep	biomarkers	1590:1599	arg1	biomarkers					1590:1599	potential biomarkers	1580:1599	potential biomarkers: betaine, N-acetyl-glycoprotein, and carnitine	1580:1646	RESULTS Three metabolites were identified as potential biomarkers: betaine, N-acetyl-glycoprotein, and carnitine.
33807002	1	74	theme	congenital	139:148	arg1	disorders					150:158	the congenital disorders	135:158	the congenital disorders	135:158	BACKGROUND ALG13-CDG belongs to the congenital disorders of glycosylation (CDG), which is an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity.
33807002	1	74	theme	congenital	139:148	arg1	group					206:210	an expanding group	193:210	an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity	193:413	BACKGROUND ALG13-CDG belongs to the congenital disorders of glycosylation (CDG), which is an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity.
33807002	1	74	theme	congenital	139:148	arg1	disorders					239:247	multisystemic metabolic disorders	215:247	multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity	215:413	BACKGROUND ALG13-CDG belongs to the congenital disorders of glycosylation (CDG), which is an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity.
33807002	8	75	theme	Nuclear	1325:1331	arg1	NMR					1353:1355	NMR	1353:1355	NMR	1353:1355	METHODS Nuclear magnetic resonance (NMR) spectroscopy in conjunction with multivariate and univariate modelling were used to analyze the metabolic profile of the blood serum samples acquired from the studied patients.
33807002	8	75	theme	Nuclear	1325:1331	arg1	resonance					1342:1350	Nuclear magnetic resonance	1325:1350	METHODS Nuclear magnetic resonance (NMR) spectroscopy in conjunction with multivariate and univariate modelling	1317:1427	METHODS Nuclear magnetic resonance (NMR) spectroscopy in conjunction with multivariate and univariate modelling were used to analyze the metabolic profile of the blood serum samples acquired from the studied patients.
33807002	1	76	theme	multisystemic	215:227	arg1	disorders					239:247	multisystemic metabolic disorders	215:247	multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity	215:413	BACKGROUND ALG13-CDG belongs to the congenital disorders of glycosylation (CDG), which is an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity.
33807002	4	77	dep	individuals	830:840	arg1	female					850:855	female	850:855	female	850:855	This manuscript describes the first metabolomic analysis using NMR in three patients with epileptic encephalopathy due to the recurrent c.320A>G variant in ALG13, characterized to date only in about 60 individuals (mostly female).
33807002	0	78	from	Analysis	21:28	arg1	Children					33:40	Children	33:40	Children with Epilepsy and ALG13-CDG	33:68	The First Metabolome Analysis in Children with Epilepsy and ALG13-CDG Resulting from c.320A>G Variant.
33807002	3	79	theme	good	588:591	arg1	individuals					546:556	Three individuals	540:556	Three individuals presented here	540:571	Three individuals presented here may serve as a good example of clinical heterogeneity.
33807002	3	79	theme	good	588:591	arg1	example					593:599	a good example	586:599	a good example of clinical heterogeneity	586:625	Three individuals presented here may serve as a good example of clinical heterogeneity.
33807002	0	80	with	Children	33:40	arg1	ALG13-CDG					60:68	ALG13-CDG	60:68	ALG13-CDG	60:68	The First Metabolome Analysis in Children with Epilepsy and ALG13-CDG Resulting from c.320A>G Variant.
33807002	0	80	with	Children	33:40	arg1	Epilepsy					47:54	Epilepsy	47:54	Epilepsy	47:54	The First Metabolome Analysis in Children with Epilepsy and ALG13-CDG Resulting from c.320A>G Variant.
33807002	2	81	theme	clinical	432:439	arg1	presentation					441:452	clinical presentation	432:452	clinical presentation	432:452	Thus, as far as clinical presentation, laboratory findings, and treatment are concerned, many questions are to be answered.
33807002	4	82	from	NMR	691:693	arg1	patients					704:711	three patients	698:711	three patients with epileptic encephalopathy due to the recurrent c.320A>G variant in ALG13	698:788	This manuscript describes the first metabolomic analysis using NMR in three patients with epileptic encephalopathy due to the recurrent c.320A>G variant in ALG13, characterized to date only in about 60 individuals (mostly female).
33807002	1	83	theme	metabolic	229:237	arg1	disorders					239:247	multisystemic metabolic disorders	215:247	multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity	215:413	BACKGROUND ALG13-CDG belongs to the congenital disorders of glycosylation (CDG), which is an expanding group of multisystemic metabolic disorders caused by the N-linked, O-linked oligosaccharides, shared substrates, glycophosphatidylinositol (GPI) anchors, and dolichols pathways with high genetic heterogeneity.
32416735	5	0	theme	%	584:584	arg1	NaCl					592:595	1 % (w/v) NaCl	582:595	1 % (w/v) NaCl	582:595	The cells grew optimally at 28 °C, pH 7.5 and with 1 % (w/v) NaCl on BHI agar supplemented with 5 % sheep blood.
32416735	6	1	theme	nearest	717:723	arg1	relative					738:745	their nearest phylogenetic relative	711:745	their nearest phylogenetic relative	711:745	Phylogenetic analysis of the 16S rRNA gene sequences revealed that their nearest phylogenetic relative was Nocardioides solisilvae Ka25T (97.9 % similarity).
32416735	2	2	theme	bacterial	148:156	arg1	dk3136T					167:173	dk3136T	167:173	dk3136T	167:173	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136T and dk3543, were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) in the Qinghai-Tibet Plateau of PR China.
32416735	2	2	theme	bacterial	148:156	arg1	strains					158:164	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains	96:164	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains	96:164	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136T and dk3543, were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) in the Qinghai-Tibet Plateau of PR China.
32416735	2	2	theme	bacterial	148:156	arg1	dk3543					179:184	dk3543	179:184	dk3543	179:184	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136T and dk3543, were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) in the Qinghai-Tibet Plateau of PR China.
32416735	10	3	theme	diamino	1314:1320	arg1	acid					1322:1325	the diagnostic diamino acid	1299:1325	the diagnostic diamino acid	1299:1325	ll-2,6-diaminopimelic acid was the diagnostic diamino acid and MK-8(H4) was the predominant respiratory quinone.
32416735	10	3	theme	diamino	1314:1320	arg1	acid					1290:1293	ll-2,6-diaminopimelic acid	1268:1293	ll-2,6-diaminopimelic acid	1268:1293	ll-2,6-diaminopimelic acid was the diagnostic diamino acid and MK-8(H4) was the predominant respiratory quinone.
32416735	8	4	theme	70.3 mol	1104:1111	arg1	%					1112:1112	70.3 mol%	1104:1112	70.3 mol%	1104:1112	The DNA G+C contents of strains dk3136T and dk3543 were 70.3 mol% and 70.4 mol%, respectively.
32416735	8	4	theme	70.3 mol	1104:1111	arg1	contents					1060:1067	The DNA G+C contents	1048:1067	The DNA G+C contents of strains dk3136T and dk3543	1048:1097	The DNA G+C contents of strains dk3136T and dk3543 were 70.3 mol% and 70.4 mol%, respectively.
32416735	6	5	theme	sequences	687:695	arg1	analysis					657:664	Phylogenetic analysis	644:664	Phylogenetic analysis of the 16S rRNA gene sequences	644:695	Phylogenetic analysis of the 16S rRNA gene sequences revealed that their nearest phylogenetic relative was Nocardioides solisilvae Ka25T (97.9 % similarity).
32416735	6	6	theme	%	787:787	arg1	Ka25T					775:779	Ka25T	775:779	Ka25T	775:779	Phylogenetic analysis of the 16S rRNA gene sequences revealed that their nearest phylogenetic relative was Nocardioides solisilvae Ka25T (97.9 % similarity).
32416735	6	6	theme	%	787:787	arg1	similarity					789:798	97.9 % similarity	782:798	97.9 % similarity	782:798	Phylogenetic analysis of the 16S rRNA gene sequences revealed that their nearest phylogenetic relative was Nocardioides solisilvae Ka25T (97.9 % similarity).
32416735	7	7	theme	nearest	1015:1021	arg1	neighbours					1036:1045	their nearest phylogenetic neighbours	1009:1045	their nearest phylogenetic neighbours	1009:1045	The results of 16S rRNA gene sequence and phylogenetic/phylogenomic analyses illustrated that N. solisilvae Ka25T, Nocardioides gilvus XZ17T, Nocardioides houyundeii 78T and Nocardioides daphniae D287T were their nearest phylogenetic neighbours.
32416735	6	8	theme	rRNA	677:680	arg1	sequences					687:695	the 16S rRNA gene sequences	669:695	the 16S rRNA gene sequences	669:695	Phylogenetic analysis of the 16S rRNA gene sequences revealed that their nearest phylogenetic relative was Nocardioides solisilvae Ka25T (97.9 % similarity).
32416735	4	9	with	agar	504:507	arg1	blood					524:528	5 % sheep blood	514:528	5 % sheep blood	514:528	Colonies were yellowish, circular without any observable aerial mycelium after culturing at 28 ℃ for 3 days on brain-heart infusion (BHI) agar with 5 % sheep blood.
32416735	12	10	contain	had	1474:1476	arg2	ω8c					1515:1517	 1 ω8c	1512:1517	 1 ω8c as the major fatty acids	1512:1542	The two strains had C18 : 1 ω9c, iso-C16 : 0 and C17 : 1 ω8c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars.
32416735	12	10	contain	had	1474:1476	arg2	rhamnose					1549:1556	rhamnose	1549:1556	rhamnose	1549:1556	The two strains had C18 : 1 ω9c, iso-C16 : 0 and C17 : 1 ω8c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars.
32416735	12	10	contain	had	1474:1476	arg2	C18 					1478:1481	C18 	1478:1481	C18 	1478:1481	The two strains had C18 : 1 ω9c, iso-C16 : 0 and C17 : 1 ω8c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars.
32416735	12	10	contain	had	1474:1476	arg2	iso-C16 					1491:1498	iso-C16 	1491:1498	iso-C16 	1491:1498	The two strains had C18 : 1 ω9c, iso-C16 : 0 and C17 : 1 ω8c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars.
32416735	12	10	contain	had	1474:1476	arg2	sugars					1595:1600	the main whole-cell sugars	1575:1600	the main whole-cell sugars	1575:1600	The two strains had C18 : 1 ω9c, iso-C16 : 0 and C17 : 1 ω8c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars.
32416735	12	10	contain	had	1474:1476	arg1	strains					1466:1472	The two strains	1458:1472	The two strains	1458:1472	The two strains had C18 : 1 ω9c, iso-C16 : 0 and C17 : 1 ω8c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars.
32416735	12	10	contain	had	1474:1476	arg2	 0					1500:1501	 0	1500:1501	 0	1500:1501	The two strains had C18 : 1 ω9c, iso-C16 : 0 and C17 : 1 ω8c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars.
32416735	12	10	contain	had	1474:1476	arg2	ω9c					1486:1488	 1 ω9c	1483:1488	 1 ω9c	1483:1488	The two strains had C18 : 1 ω9c, iso-C16 : 0 and C17 : 1 ω8c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars.
32416735	2	11	theme	Qinghai-Tibet	267:279	arg1	Plateau					281:287	the Qinghai-Tibet Plateau	263:287	the Qinghai-Tibet Plateau of PR China	263:299	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136T and dk3543, were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) in the Qinghai-Tibet Plateau of PR China.
32416735	12	12	theme	 1	1512:1513	arg1	 0					1500:1501	 0	1500:1501	 0	1500:1501	The two strains had C18 : 1 ω9c, iso-C16 : 0 and C17 : 1 ω8c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars.
32416735	12	12	theme	 1	1512:1513	arg1	sugars					1595:1600	the main whole-cell sugars	1575:1600	the main whole-cell sugars	1575:1600	The two strains had C18 : 1 ω9c, iso-C16 : 0 and C17 : 1 ω8c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars.
32416735	12	12	theme	 1	1512:1513	arg1	ω9c					1486:1488	 1 ω9c	1483:1488	 1 ω9c	1483:1488	The two strains had C18 : 1 ω9c, iso-C16 : 0 and C17 : 1 ω8c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars.
32416735	12	12	theme	 1	1512:1513	arg1	ω8c					1515:1517	 1 ω8c	1512:1517	 1 ω8c as the major fatty acids	1512:1542	The two strains had C18 : 1 ω9c, iso-C16 : 0 and C17 : 1 ω8c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars.
32416735	12	12	theme	 1	1512:1513	arg1	C18 					1478:1481	C18 	1478:1481	C18 	1478:1481	The two strains had C18 : 1 ω9c, iso-C16 : 0 and C17 : 1 ω8c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars.
32416735	12	12	theme	 1	1512:1513	arg1	rhamnose					1549:1556	rhamnose	1549:1556	rhamnose	1549:1556	The two strains had C18 : 1 ω9c, iso-C16 : 0 and C17 : 1 ω8c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars.
32416735	5	13	theme	5 	627:628	arg1	%					629:629	%	629:629	%	629:629	The cells grew optimally at 28 °C, pH 7.5 and with 1 % (w/v) NaCl on BHI agar supplemented with 5 % sheep blood.
32416735	9	14	theme	lower	1165:1169	arg1	identity					1215:1222	lower than threshold (95-96 %) average nucleotide identity	1165:1222	lower than threshold (95-96 %) average nucleotide identity to known species of the genus Nocardioides	1165:1265	Their genomes exhibit lower than threshold (95-96 %) average nucleotide identity to known species of the genus Nocardioides.
32416735	9	15	theme	threshold	1176:1184	arg1	identity					1215:1222	lower than threshold (95-96 %) average nucleotide identity	1165:1222	lower than threshold (95-96 %) average nucleotide identity to known species of the genus Nocardioides	1165:1265	Their genomes exhibit lower than threshold (95-96 %) average nucleotide identity to known species of the genus Nocardioides.
32416735	12	16	theme	whole-cell	1584:1593	arg1	 0					1500:1501	 0	1500:1501	 0	1500:1501	The two strains had C18 : 1 ω9c, iso-C16 : 0 and C17 : 1 ω8c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars.
32416735	12	16	theme	whole-cell	1584:1593	arg1	sugars					1595:1600	the main whole-cell sugars	1575:1600	the main whole-cell sugars	1575:1600	The two strains had C18 : 1 ω9c, iso-C16 : 0 and C17 : 1 ω8c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars.
32416735	12	16	theme	whole-cell	1584:1593	arg1	ω9c					1486:1488	 1 ω9c	1483:1488	 1 ω9c	1483:1488	The two strains had C18 : 1 ω9c, iso-C16 : 0 and C17 : 1 ω8c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars.
32416735	12	16	theme	whole-cell	1584:1593	arg1	ω8c					1515:1517	 1 ω8c	1512:1517	 1 ω8c as the major fatty acids	1512:1542	The two strains had C18 : 1 ω9c, iso-C16 : 0 and C17 : 1 ω8c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars.
32416735	12	16	theme	whole-cell	1584:1593	arg1	C18 					1478:1481	C18 	1478:1481	C18 	1478:1481	The two strains had C18 : 1 ω9c, iso-C16 : 0 and C17 : 1 ω8c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars.
32416735	12	16	theme	whole-cell	1584:1593	arg1	rhamnose					1549:1556	rhamnose	1549:1556	rhamnose	1549:1556	The two strains had C18 : 1 ω9c, iso-C16 : 0 and C17 : 1 ω8c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars.
32416735	5	17	theme	BHI	600:602	arg1	agar					604:607	BHI agar	600:607	BHI agar supplemented with 5 % sheep blood	600:641	The cells grew optimally at 28 °C, pH 7.5 and with 1 % (w/v) NaCl on BHI agar supplemented with 5 % sheep blood.
32416735	9	18	theme	95-96 	1187:1192	arg1	identity					1215:1222	lower than threshold (95-96 %) average nucleotide identity	1165:1222	lower than threshold (95-96 %) average nucleotide identity to known species of the genus Nocardioides	1165:1265	Their genomes exhibit lower than threshold (95-96 %) average nucleotide identity to known species of the genus Nocardioides.
32416735	4	19	theme	observable	412:421	arg1	mycelium					430:437	any observable aerial mycelium	408:437	any observable aerial mycelium	408:437	Colonies were yellowish, circular without any observable aerial mycelium after culturing at 28 ℃ for 3 days on brain-heart infusion (BHI) agar with 5 % sheep blood.
32416735	2	20	theme	Tibetan	220:226	arg1	gazelle					228:234	Tibetan gazelle	220:234	Tibetan gazelle	220:234	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136T and dk3543, were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) in the Qinghai-Tibet Plateau of PR China.
32416735	10	21	theme	respiratory	1360:1370	arg1	MK-8					1331:1334	MK-8	1331:1334	MK-8(H4)	1331:1338	ll-2,6-diaminopimelic acid was the diagnostic diamino acid and MK-8(H4) was the predominant respiratory quinone.
32416735	10	21	theme	respiratory	1360:1370	arg1	quinone					1372:1378	the predominant respiratory quinone	1344:1378	the predominant respiratory quinone	1344:1378	ll-2,6-diaminopimelic acid was the diagnostic diamino acid and MK-8(H4) was the predominant respiratory quinone.
32416735	9	22	theme	nucleotide	1204:1213	arg1	identity					1215:1222	lower than threshold (95-96 %) average nucleotide identity	1165:1222	lower than threshold (95-96 %) average nucleotide identity to known species of the genus Nocardioides	1165:1265	Their genomes exhibit lower than threshold (95-96 %) average nucleotide identity to known species of the genus Nocardioides.
32416735	4	23	theme	infusion	489:496	arg1	agar					504:507	brain-heart infusion (BHI) agar	477:507	brain-heart infusion (BHI) agar with 5 % sheep blood	477:528	Colonies were yellowish, circular without any observable aerial mycelium after culturing at 28 ℃ for 3 days on brain-heart infusion (BHI) agar with 5 % sheep blood.
32416735	13	24	theme	genus	1759:1763	arg1	Nocardioides					1765:1776	genus Nocardioides	1759:1776	genus Nocardioides	1759:1776	On the basis of the results of our genotypic, phenotypic and biochemical analyses, we conclude that strains dk3136T and dk3543 represent a novel species in genus Nocardioides, for which the name Nocardioides jishulii sp.
32416735	5	25	theme	sheep	631:635	arg1	blood					637:641	5 % sheep blood	627:641	5 % sheep blood	627:641	The cells grew optimally at 28 °C, pH 7.5 and with 1 % (w/v) NaCl on BHI agar supplemented with 5 % sheep blood.
32416735	13	26	dep	results	1623:1629	arg1	basis					1610:1614	basis	1610:1614	basis	1610:1614	On the basis of the results of our genotypic, phenotypic and biochemical analyses, we conclude that strains dk3136T and dk3543 represent a novel species in genus Nocardioides, for which the name Nocardioides jishulii sp.
32416735	13	26	dep	results	1623:1629	arg1	the					1606:1608	the	1606:1608	the	1606:1608	On the basis of the results of our genotypic, phenotypic and biochemical analyses, we conclude that strains dk3136T and dk3543 represent a novel species in genus Nocardioides, for which the name Nocardioides jishulii sp.
32416735	7	27	theme	phylogenetic/phylogenomic	844:868	arg1	analyses					870:877	phylogenetic/phylogenomic analyses	844:877	phylogenetic/phylogenomic analyses	844:877	The results of 16S rRNA gene sequence and phylogenetic/phylogenomic analyses illustrated that N. solisilvae Ka25T, Nocardioides gilvus XZ17T, Nocardioides houyundeii 78T and Nocardioides daphniae D287T were their nearest phylogenetic neighbours.
32416735	7	28	theme	sequence	831:838	arg1	results					806:812	The results	802:812	The results of 16S rRNA gene sequence and phylogenetic/phylogenomic analyses	802:877	The results of 16S rRNA gene sequence and phylogenetic/phylogenomic analyses illustrated that N. solisilvae Ka25T, Nocardioides gilvus XZ17T, Nocardioides houyundeii 78T and Nocardioides daphniae D287T were their nearest phylogenetic neighbours.
32416735	1	29	theme	gazelle	63:69	arg1	faeces					45:50	faeces	45:50	faeces of Tibetan gazelle (Procapra picticaudata)	45:93	nov.,isolated from faeces of Tibetan gazelle (Procapra picticaudata).
32416735	2	30	attach	isolated	192:199	arg1	faeces					210:215	the faeces	206:215	the faeces of Tibetan gazelle	206:234	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136T and dk3543, were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) in the Qinghai-Tibet Plateau of PR China.
32416735	2	30	attach	isolated	192:199	arg2	strains					158:164	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains	96:164	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains	96:164	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136T and dk3543, were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) in the Qinghai-Tibet Plateau of PR China.
32416735	2	30	attach	isolated	192:199	arg2	dk3543					179:184	dk3543	179:184	dk3543	179:184	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136T and dk3543, were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) in the Qinghai-Tibet Plateau of PR China.
32416735	2	30	attach	isolated	192:199	arg2	dk3136T					167:173	dk3136T	167:173	dk3136T	167:173	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136T and dk3543, were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) in the Qinghai-Tibet Plateau of PR China.
32416735	7	31	theme	rRNA	821:824	arg1	sequence					831:838	16S rRNA gene sequence	817:838	16S rRNA gene sequence	817:838	The results of 16S rRNA gene sequence and phylogenetic/phylogenomic analyses illustrated that N. solisilvae Ka25T, Nocardioides gilvus XZ17T, Nocardioides houyundeii 78T and Nocardioides daphniae D287T were their nearest phylogenetic neighbours.
32416735	7	32	theme	phylogenetic	1023:1034	arg1	neighbours					1036:1045	their nearest phylogenetic neighbours	1009:1045	their nearest phylogenetic neighbours	1009:1045	The results of 16S rRNA gene sequence and phylogenetic/phylogenomic analyses illustrated that N. solisilvae Ka25T, Nocardioides gilvus XZ17T, Nocardioides houyundeii 78T and Nocardioides daphniae D287T were their nearest phylogenetic neighbours.
32416735	4	33	theme	5 	514:515	arg1	%					516:516	%	516:516	%	516:516	Colonies were yellowish, circular without any observable aerial mycelium after culturing at 28 ℃ for 3 days on brain-heart infusion (BHI) agar with 5 % sheep blood.
32416735	11	34	theme	major	1385:1389	arg1	diphosphatidylglycerol					1409:1430	diphosphatidylglycerol	1409:1430	diphosphatidylglycerol	1409:1430	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol.
32416735	11	34	theme	major	1385:1389	arg1	lipids					1397:1402	The major polar lipids	1381:1402	The major polar lipids	1381:1402	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol.
32416735	9	35	theme	Nocardioides	1254:1265	arg1	species					1233:1239	known species	1227:1239	known species of the genus Nocardioides	1227:1265	Their genomes exhibit lower than threshold (95-96 %) average nucleotide identity to known species of the genus Nocardioides.
32416735	6	36	theme	97.9 	782:786	arg1	%					787:787	%	787:787	%	787:787	Phylogenetic analysis of the 16S rRNA gene sequences revealed that their nearest phylogenetic relative was Nocardioides solisilvae Ka25T (97.9 % similarity).
32416735	15	37	theme	4.7570T=JCM	1877:1887	arg1	49314T					1901:1906	=CGMCC 4.7570T=JCM 33496T=KCTC 49314T	1870:1906	=CGMCC 4.7570T=JCM 33496T=KCTC 49314T	1870:1906	The type strain is dk3136T (=CGMCC 4.7570T=JCM 33496T=KCTC 49314T).
32416735	15	37	theme	4.7570T=JCM	1877:1887	arg1	dk3136T					1861:1867	dk3136T	1861:1867	dk3136T (=CGMCC 4.7570T=JCM 33496T=KCTC 49314T)	1861:1907	The type strain is dk3136T (=CGMCC 4.7570T=JCM 33496T=KCTC 49314T).
32416735	12	38	theme	fatty	1532:1536	arg1	acids					1538:1542	the major fatty acids	1522:1542	the major fatty acids	1522:1542	The two strains had C18 : 1 ω9c, iso-C16 : 0 and C17 : 1 ω8c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars.
32416735	4	39	theme	sheep	518:522	arg1	blood					524:528	5 % sheep blood	514:528	5 % sheep blood	514:528	Colonies were yellowish, circular without any observable aerial mycelium after culturing at 28 ℃ for 3 days on brain-heart infusion (BHI) agar with 5 % sheep blood.
32416735	4	40	theme	BHI	499:501	arg1	agar					504:507	brain-heart infusion (BHI) agar	477:507	brain-heart infusion (BHI) agar with 5 % sheep blood	477:528	Colonies were yellowish, circular without any observable aerial mycelium after culturing at 28 ℃ for 3 days on brain-heart infusion (BHI) agar with 5 % sheep blood.
32416735	6	41	theme	Phylogenetic	644:655	arg1	analysis					657:664	Phylogenetic analysis	644:664	Phylogenetic analysis of the 16S rRNA gene sequences	644:695	Phylogenetic analysis of the 16S rRNA gene sequences revealed that their nearest phylogenetic relative was Nocardioides solisilvae Ka25T (97.9 % similarity).
32416735	7	42	theme	16S	817:819	arg1	rRNA					821:824	16S rRNA	817:824	16S rRNA gene sequence	817:838	The results of 16S rRNA gene sequence and phylogenetic/phylogenomic analyses illustrated that N. solisilvae Ka25T, Nocardioides gilvus XZ17T, Nocardioides houyundeii 78T and Nocardioides daphniae D287T were their nearest phylogenetic neighbours.
32416735	8	43	theme	DNA	1052:1054	arg1	contents					1060:1067	The DNA G+C contents	1048:1067	The DNA G+C contents of strains dk3136T and dk3543	1048:1097	The DNA G+C contents of strains dk3136T and dk3543 were 70.3 mol% and 70.4 mol%, respectively.
32416735	8	43	theme	DNA	1052:1054	arg1	%					1112:1112	70.3 mol%	1104:1112	70.3 mol%	1104:1112	The DNA G+C contents of strains dk3136T and dk3543 were 70.3 mol% and 70.4 mol%, respectively.
32416735	0	44	dep	sp	22:23	arg1	Nocardioides					0:11	Nocardioides	0:11	Nocardioides	0:11	Nocardioides jishulii sp.
32416735	13	45	dep	strains	1703:1709	arg1	strains					1703:1709	strains	1703:1709	strains dk3136T and dk3543	1703:1728	On the basis of the results of our genotypic, phenotypic and biochemical analyses, we conclude that strains dk3136T and dk3543 represent a novel species in genus Nocardioides, for which the name Nocardioides jishulii sp.
32416735	13	45	dep	strains	1703:1709	arg1	dk3543					1723:1728	dk3543	1723:1728	dk3543	1723:1728	On the basis of the results of our genotypic, phenotypic and biochemical analyses, we conclude that strains dk3136T and dk3543 represent a novel species in genus Nocardioides, for which the name Nocardioides jishulii sp.
32416735	13	45	dep	strains	1703:1709	arg1	dk3136T					1711:1717	dk3136T	1711:1717	dk3136T	1711:1717	On the basis of the results of our genotypic, phenotypic and biochemical analyses, we conclude that strains dk3136T and dk3543 represent a novel species in genus Nocardioides, for which the name Nocardioides jishulii sp.
32416735	13	46	theme	biochemical	1664:1674	arg1	analyses					1676:1683	our genotypic, phenotypic and biochemical analyses	1634:1683	our genotypic, phenotypic and biochemical analyses	1634:1683	On the basis of the results of our genotypic, phenotypic and biochemical analyses, we conclude that strains dk3136T and dk3543 represent a novel species in genus Nocardioides, for which the name Nocardioides jishulii sp.
32416735	2	47	theme	novel	100:104	arg1	dk3136T					167:173	dk3136T	167:173	dk3136T	167:173	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136T and dk3543, were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) in the Qinghai-Tibet Plateau of PR China.
32416735	2	47	theme	novel	100:104	arg1	strains					158:164	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains	96:164	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains	96:164	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136T and dk3543, were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) in the Qinghai-Tibet Plateau of PR China.
32416735	2	47	theme	novel	100:104	arg1	dk3543					179:184	dk3543	179:184	dk3543	179:184	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136T and dk3543, were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) in the Qinghai-Tibet Plateau of PR China.
32416735	5	48	theme	1 	582:583	arg1	%					584:584	%	584:584	%	584:584	The cells grew optimally at 28 °C, pH 7.5 and with 1 % (w/v) NaCl on BHI agar supplemented with 5 % sheep blood.
32416735	10	49	theme	ll-2,6-diaminopimelic	1268:1288	arg1	acid					1322:1325	the diagnostic diamino acid	1299:1325	the diagnostic diamino acid	1299:1325	ll-2,6-diaminopimelic acid was the diagnostic diamino acid and MK-8(H4) was the predominant respiratory quinone.
32416735	10	49	theme	ll-2,6-diaminopimelic	1268:1288	arg1	acid					1290:1293	ll-2,6-diaminopimelic acid	1268:1293	ll-2,6-diaminopimelic acid	1268:1293	ll-2,6-diaminopimelic acid was the diagnostic diamino acid and MK-8(H4) was the predominant respiratory quinone.
32416735	8	50	theme	strains	1072:1078	arg1	contents					1060:1067	The DNA G+C contents	1048:1067	The DNA G+C contents of strains dk3136T and dk3543	1048:1097	The DNA G+C contents of strains dk3136T and dk3543 were 70.3 mol% and 70.4 mol%, respectively.
32416735	8	50	theme	strains	1072:1078	arg1	%					1112:1112	70.3 mol%	1104:1112	70.3 mol%	1104:1112	The DNA G+C contents of strains dk3136T and dk3543 were 70.3 mol% and 70.4 mol%, respectively.
32416735	13	51	theme	genotypic	1638:1646	arg1	analyses					1676:1683	our genotypic, phenotypic and biochemical analyses	1634:1683	our genotypic, phenotypic and biochemical analyses	1634:1683	On the basis of the results of our genotypic, phenotypic and biochemical analyses, we conclude that strains dk3136T and dk3543 represent a novel species in genus Nocardioides, for which the name Nocardioides jishulii sp.
32416735	13	52	theme	name	1793:1796	arg1	sp					1820:1821	the name Nocardioides jishulii sp	1789:1821	the name Nocardioides jishulii sp	1789:1821	On the basis of the results of our genotypic, phenotypic and biochemical analyses, we conclude that strains dk3136T and dk3543 represent a novel species in genus Nocardioides, for which the name Nocardioides jishulii sp.
32416735	4	53	theme	circular	391:398	arg1	yellowish					380:388	yellowish	380:388	yellowish	380:388	Colonies were yellowish, circular without any observable aerial mycelium after culturing at 28 ℃ for 3 days on brain-heart infusion (BHI) agar with 5 % sheep blood.
32416735	13	54	theme	phenotypic	1649:1658	arg1	analyses					1676:1683	our genotypic, phenotypic and biochemical analyses	1634:1683	our genotypic, phenotypic and biochemical analyses	1634:1683	On the basis of the results of our genotypic, phenotypic and biochemical analyses, we conclude that strains dk3136T and dk3543 represent a novel species in genus Nocardioides, for which the name Nocardioides jishulii sp.
32416735	2	55	theme	rod-shaped	137:146	arg1	dk3136T					167:173	dk3136T	167:173	dk3136T	167:173	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136T and dk3543, were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) in the Qinghai-Tibet Plateau of PR China.
32416735	2	55	theme	rod-shaped	137:146	arg1	strains					158:164	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains	96:164	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains	96:164	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136T and dk3543, were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) in the Qinghai-Tibet Plateau of PR China.
32416735	2	55	theme	rod-shaped	137:146	arg1	dk3543					179:184	dk3543	179:184	dk3543	179:184	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136T and dk3543, were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) in the Qinghai-Tibet Plateau of PR China.
32416735	4	56	from	3 days	467:472	arg1	agar					504:507	brain-heart infusion (BHI) agar	477:507	brain-heart infusion (BHI) agar with 5 % sheep blood	477:528	Colonies were yellowish, circular without any observable aerial mycelium after culturing at 28 ℃ for 3 days on brain-heart infusion (BHI) agar with 5 % sheep blood.
32416735	10	57	theme	diagnostic	1303:1312	arg1	acid					1322:1325	the diagnostic diamino acid	1299:1325	the diagnostic diamino acid	1299:1325	ll-2,6-diaminopimelic acid was the diagnostic diamino acid and MK-8(H4) was the predominant respiratory quinone.
32416735	10	57	theme	diagnostic	1303:1312	arg1	acid					1290:1293	ll-2,6-diaminopimelic acid	1268:1293	ll-2,6-diaminopimelic acid	1268:1293	ll-2,6-diaminopimelic acid was the diagnostic diamino acid and MK-8(H4) was the predominant respiratory quinone.
32416735	6	58	theme	gene	682:685	arg1	sequences					687:695	the 16S rRNA gene sequences	669:695	the 16S rRNA gene sequences	669:695	Phylogenetic analysis of the 16S rRNA gene sequences revealed that their nearest phylogenetic relative was Nocardioides solisilvae Ka25T (97.9 % similarity).
32416735	2	59	theme	PR	292:293	arg1	China					295:299	PR China	292:299	PR China	292:299	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136T and dk3543, were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) in the Qinghai-Tibet Plateau of PR China.
32416735	13	60	theme	Nocardioides	1798:1809	arg1	sp					1820:1821	the name Nocardioides jishulii sp	1789:1821	the name Nocardioides jishulii sp	1789:1821	On the basis of the results of our genotypic, phenotypic and biochemical analyses, we conclude that strains dk3136T and dk3543 represent a novel species in genus Nocardioides, for which the name Nocardioides jishulii sp.
32416735	13	61	theme	jishulii	1811:1818	arg1	sp					1820:1821	the name Nocardioides jishulii sp	1789:1821	the name Nocardioides jishulii sp	1789:1821	On the basis of the results of our genotypic, phenotypic and biochemical analyses, we conclude that strains dk3136T and dk3543 represent a novel species in genus Nocardioides, for which the name Nocardioides jishulii sp.
32416735	8	62	theme	70.4 mol	1118:1125	arg1	%					1126:1126	70.4 mol%	1118:1126	70.4 mol%	1118:1126	The DNA G+C contents of strains dk3136T and dk3543 were 70.3 mol% and 70.4 mol%, respectively.
32416735	9	63	theme	than	1171:1174	arg1	identity					1215:1222	lower than threshold (95-96 %) average nucleotide identity	1165:1222	lower than threshold (95-96 %) average nucleotide identity to known species of the genus Nocardioides	1165:1265	Their genomes exhibit lower than threshold (95-96 %) average nucleotide identity to known species of the genus Nocardioides.
32416735	6	64	theme	16S	673:675	arg1	sequences					687:695	the 16S rRNA gene sequences	669:695	the 16S rRNA gene sequences	669:695	Phylogenetic analysis of the 16S rRNA gene sequences revealed that their nearest phylogenetic relative was Nocardioides solisilvae Ka25T (97.9 % similarity).
32416735	12	65	theme	main	1579:1582	arg1	 0					1500:1501	 0	1500:1501	 0	1500:1501	The two strains had C18 : 1 ω9c, iso-C16 : 0 and C17 : 1 ω8c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars.
32416735	12	65	theme	main	1579:1582	arg1	sugars					1595:1600	the main whole-cell sugars	1575:1600	the main whole-cell sugars	1575:1600	The two strains had C18 : 1 ω9c, iso-C16 : 0 and C17 : 1 ω8c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars.
32416735	12	65	theme	main	1579:1582	arg1	ω9c					1486:1488	 1 ω9c	1483:1488	 1 ω9c	1483:1488	The two strains had C18 : 1 ω9c, iso-C16 : 0 and C17 : 1 ω8c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars.
32416735	12	65	theme	main	1579:1582	arg1	ω8c					1515:1517	 1 ω8c	1512:1517	 1 ω8c as the major fatty acids	1512:1542	The two strains had C18 : 1 ω9c, iso-C16 : 0 and C17 : 1 ω8c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars.
32416735	12	65	theme	main	1579:1582	arg1	C18 					1478:1481	C18 	1478:1481	C18 	1478:1481	The two strains had C18 : 1 ω9c, iso-C16 : 0 and C17 : 1 ω8c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars.
32416735	12	65	theme	main	1579:1582	arg1	rhamnose					1549:1556	rhamnose	1549:1556	rhamnose	1549:1556	The two strains had C18 : 1 ω9c, iso-C16 : 0 and C17 : 1 ω8c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars.
32416735	4	66	theme	aerial	423:428	arg1	mycelium					430:437	any observable aerial mycelium	408:437	any observable aerial mycelium	408:437	Colonies were yellowish, circular without any observable aerial mycelium after culturing at 28 ℃ for 3 days on brain-heart infusion (BHI) agar with 5 % sheep blood.
32416735	11	67	theme	polar	1391:1395	arg1	diphosphatidylglycerol					1409:1430	diphosphatidylglycerol	1409:1430	diphosphatidylglycerol	1409:1430	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol.
32416735	11	67	theme	polar	1391:1395	arg1	lipids					1397:1402	The major polar lipids	1381:1402	The major polar lipids	1381:1402	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol.
32416735	9	68	theme	%	1193:1193	arg1	identity					1215:1222	lower than threshold (95-96 %) average nucleotide identity	1165:1222	lower than threshold (95-96 %) average nucleotide identity to known species of the genus Nocardioides	1165:1265	Their genomes exhibit lower than threshold (95-96 %) average nucleotide identity to known species of the genus Nocardioides.
32416735	2	69	theme	gazelle	228:234	arg1	faeces					210:215	the faeces	206:215	the faeces of Tibetan gazelle	206:234	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136T and dk3543, were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) in the Qinghai-Tibet Plateau of PR China.
32416735	5	70	from	28 °C	559:563	arg1	agar					604:607	BHI agar	600:607	BHI agar supplemented with 5 % sheep blood	600:641	The cells grew optimally at 28 °C, pH 7.5 and with 1 % (w/v) NaCl on BHI agar supplemented with 5 % sheep blood.
32416735	4	71	theme	brain-heart	477:487	arg1	agar					504:507	brain-heart infusion (BHI) agar	477:507	brain-heart infusion (BHI) agar with 5 % sheep blood	477:528	Colonies were yellowish, circular without any observable aerial mycelium after culturing at 28 ℃ for 3 days on brain-heart infusion (BHI) agar with 5 % sheep blood.
32416735	13	72	theme	novel	1742:1746	arg1	species					1748:1754	a novel species	1740:1754	a novel species	1740:1754	On the basis of the results of our genotypic, phenotypic and biochemical analyses, we conclude that strains dk3136T and dk3543 represent a novel species in genus Nocardioides, for which the name Nocardioides jishulii sp.
32416735	9	73	theme	average	1196:1202	arg1	identity					1215:1222	lower than threshold (95-96 %) average nucleotide identity	1165:1222	lower than threshold (95-96 %) average nucleotide identity to known species of the genus Nocardioides	1165:1265	Their genomes exhibit lower than threshold (95-96 %) average nucleotide identity to known species of the genus Nocardioides.
32416735	10	74	theme	predominant	1348:1358	arg1	MK-8					1331:1334	MK-8	1331:1334	MK-8(H4)	1331:1338	ll-2,6-diaminopimelic acid was the diagnostic diamino acid and MK-8(H4) was the predominant respiratory quinone.
32416735	10	74	theme	predominant	1348:1358	arg1	quinone					1372:1378	the predominant respiratory quinone	1344:1378	the predominant respiratory quinone	1344:1378	ll-2,6-diaminopimelic acid was the diagnostic diamino acid and MK-8(H4) was the predominant respiratory quinone.
32416735	5	75	theme	%	629:629	arg1	blood					637:641	5 % sheep blood	627:641	5 % sheep blood	627:641	The cells grew optimally at 28 °C, pH 7.5 and with 1 % (w/v) NaCl on BHI agar supplemented with 5 % sheep blood.
32416735	9	76	theme	known	1227:1231	arg1	species					1233:1239	known species	1227:1239	known species of the genus Nocardioides	1227:1265	Their genomes exhibit lower than threshold (95-96 %) average nucleotide identity to known species of the genus Nocardioides.
32416735	7	77	theme	analyses	870:877	arg1	results					806:812	The results	802:812	The results of 16S rRNA gene sequence and phylogenetic/phylogenomic analyses	802:877	The results of 16S rRNA gene sequence and phylogenetic/phylogenomic analyses illustrated that N. solisilvae Ka25T, Nocardioides gilvus XZ17T, Nocardioides houyundeii 78T and Nocardioides daphniae D287T were their nearest phylogenetic neighbours.
32416735	1	78	theme	Tibetan	55:61	arg1	gazelle					63:69	Tibetan gazelle	55:69	Tibetan gazelle (Procapra picticaudata)	55:93	nov.,isolated from faeces of Tibetan gazelle (Procapra picticaudata).
32416735	1	78	theme	Tibetan	55:61	arg1	picticaudata					81:92	picticaudata	81:92	picticaudata	81:92	nov.,isolated from faeces of Tibetan gazelle (Procapra picticaudata).
32416735	8	79	dep	strains	1072:1078	arg1	strains					1072:1078	strains dk3136T and dk3543	1072:1097	strains dk3136T and dk3543	1072:1097	The DNA G+C contents of strains dk3136T and dk3543 were 70.3 mol% and 70.4 mol%, respectively.
32416735	8	79	dep	strains	1072:1078	arg1	dk3543					1092:1097	dk3543	1092:1097	dk3543	1092:1097	The DNA G+C contents of strains dk3136T and dk3543 were 70.3 mol% and 70.4 mol%, respectively.
32416735	8	79	dep	strains	1072:1078	arg1	dk3136T					1080:1086	dk3136T	1080:1086	dk3136T	1080:1086	The DNA G+C contents of strains dk3136T and dk3543 were 70.3 mol% and 70.4 mol%, respectively.
32416735	0	80	theme	jishulii	13:20	arg1	sp					22:23	jishulii sp	13:23	jishulii sp	13:23	Nocardioides jishulii sp.
32416735	15	81	theme	33496T=KCTC	1889:1899	arg1	49314T					1901:1906	=CGMCC 4.7570T=JCM 33496T=KCTC 49314T	1870:1906	=CGMCC 4.7570T=JCM 33496T=KCTC 49314T	1870:1906	The type strain is dk3136T (=CGMCC 4.7570T=JCM 33496T=KCTC 49314T).
32416735	15	81	theme	33496T=KCTC	1889:1899	arg1	dk3136T					1861:1867	dk3136T	1861:1867	dk3136T (=CGMCC 4.7570T=JCM 33496T=KCTC 49314T)	1861:1907	The type strain is dk3136T (=CGMCC 4.7570T=JCM 33496T=KCTC 49314T).
32416735	4	82	theme	%	516:516	arg1	blood					524:528	5 % sheep blood	514:528	5 % sheep blood	514:528	Colonies were yellowish, circular without any observable aerial mycelium after culturing at 28 ℃ for 3 days on brain-heart infusion (BHI) agar with 5 % sheep blood.
32416735	9	83	theme	genus	1248:1252	arg1	Nocardioides					1254:1265	the genus Nocardioides	1244:1265	the genus Nocardioides	1244:1265	Their genomes exhibit lower than threshold (95-96 %) average nucleotide identity to known species of the genus Nocardioides.
32416735	7	84	theme	gene	826:829	arg1	sequence					831:838	16S rRNA gene sequence	817:838	16S rRNA gene sequence	817:838	The results of 16S rRNA gene sequence and phylogenetic/phylogenomic analyses illustrated that N. solisilvae Ka25T, Nocardioides gilvus XZ17T, Nocardioides houyundeii 78T and Nocardioides daphniae D287T were their nearest phylogenetic neighbours.
32416735	15	85	theme	=CGMCC	1870:1875	arg1	49314T					1901:1906	=CGMCC 4.7570T=JCM 33496T=KCTC 49314T	1870:1906	=CGMCC 4.7570T=JCM 33496T=KCTC 49314T	1870:1906	The type strain is dk3136T (=CGMCC 4.7570T=JCM 33496T=KCTC 49314T).
32416735	15	85	theme	=CGMCC	1870:1875	arg1	dk3136T					1861:1867	dk3136T	1861:1867	dk3136T (=CGMCC 4.7570T=JCM 33496T=KCTC 49314T)	1861:1907	The type strain is dk3136T (=CGMCC 4.7570T=JCM 33496T=KCTC 49314T).
32416735	5	86	dep	%	584:584	arg1	w/v					587:589	w/v	587:589	w/v	587:589	The cells grew optimally at 28 °C, pH 7.5 and with 1 % (w/v) NaCl on BHI agar supplemented with 5 % sheep blood.
32416735	12	87	theme	major	1526:1530	arg1	acids					1538:1542	the major fatty acids	1522:1542	the major fatty acids	1522:1542	The two strains had C18 : 1 ω9c, iso-C16 : 0 and C17 : 1 ω8c as the major fatty acids, and rhamnose and galactose as the main whole-cell sugars.
32416735	2	88	dep	Gram-stain-positive	106:124	arg1	irregular					127:135	irregular	127:135	irregular	127:135	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136T and dk3543, were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) in the Qinghai-Tibet Plateau of PR China.
32416735	15	89	theme	type	1846:1849	arg1	dk3136T					1861:1867	dk3136T	1861:1867	dk3136T (=CGMCC 4.7570T=JCM 33496T=KCTC 49314T)	1861:1907	The type strain is dk3136T (=CGMCC 4.7570T=JCM 33496T=KCTC 49314T).
32416735	15	89	theme	type	1846:1849	arg1	strain					1851:1856	The type strain	1842:1856	The type strain	1842:1856	The type strain is dk3136T (=CGMCC 4.7570T=JCM 33496T=KCTC 49314T).
32416735	8	90	theme	G+C	1056:1058	arg1	contents					1060:1067	The DNA G+C contents	1048:1067	The DNA G+C contents of strains dk3136T and dk3543	1048:1097	The DNA G+C contents of strains dk3136T and dk3543 were 70.3 mol% and 70.4 mol%, respectively.
32416735	8	90	theme	G+C	1056:1058	arg1	%					1112:1112	70.3 mol%	1104:1112	70.3 mol%	1104:1112	The DNA G+C contents of strains dk3136T and dk3543 were 70.3 mol% and 70.4 mol%, respectively.
32416735	13	91	from	species	1748:1754	arg1	Nocardioides					1765:1776	genus Nocardioides	1759:1776	genus Nocardioides	1759:1776	On the basis of the results of our genotypic, phenotypic and biochemical analyses, we conclude that strains dk3136T and dk3543 represent a novel species in genus Nocardioides, for which the name Nocardioides jishulii sp.
32416735	2	92	theme	China	295:299	arg1	Plateau					281:287	the Qinghai-Tibet Plateau	263:287	the Qinghai-Tibet Plateau of PR China	263:299	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136T and dk3543, were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) in the Qinghai-Tibet Plateau of PR China.
32416735	6	93	theme	phylogenetic	725:736	arg1	relative					738:745	their nearest phylogenetic relative	711:745	their nearest phylogenetic relative	711:745	Phylogenetic analysis of the 16S rRNA gene sequences revealed that their nearest phylogenetic relative was Nocardioides solisilvae Ka25T (97.9 % similarity).
32416735	2	94	dep	isolated	192:199	arg1	picticaudata					246:257	picticaudata	246:257	picticaudata	246:257	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136T and dk3543, were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) in the Qinghai-Tibet Plateau of PR China.
32416735	13	95	theme	analyses	1676:1683	arg1	results					1623:1629	the results	1619:1629	the results of our genotypic, phenotypic and biochemical analyses	1619:1683	On the basis of the results of our genotypic, phenotypic and biochemical analyses, we conclude that strains dk3136T and dk3543 represent a novel species in genus Nocardioides, for which the name Nocardioides jishulii sp.
32416735	2	96	theme	Gram-stain-positive	106:124	arg1	dk3136T					167:173	dk3136T	167:173	dk3136T	167:173	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136T and dk3543, were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) in the Qinghai-Tibet Plateau of PR China.
32416735	2	96	theme	Gram-stain-positive	106:124	arg1	strains					158:164	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains	96:164	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains	96:164	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136T and dk3543, were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) in the Qinghai-Tibet Plateau of PR China.
32416735	2	96	theme	Gram-stain-positive	106:124	arg1	dk3543					179:184	dk3543	179:184	dk3543	179:184	Two novel Gram-stain-positive, irregular rod-shaped bacterial strains, dk3136T and dk3543, were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) in the Qinghai-Tibet Plateau of PR China.
32375949	14	0	theme	fatty	1374:1378	arg1	acids					1380:1384	Major fatty acids	1368:1384	Major fatty acids	1368:1384	Major fatty acids were iso-C16 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
32375949	18	1	theme	type	1947:1950	arg1	strain					1952:1957	The type strain	1943:1957	The type strain	1943:1957	The type strain is EGI 650086T (=KCTC 49044T=CGMCC 4.7188T).
32375949	18	1	theme	type	1947:1950	arg1	650086T					1966:1972	EGI 650086T	1962:1972	EGI 650086T (=KCTC 49044T=CGMCC 4.7188T)	1962:2001	The type strain is EGI 650086T (=KCTC 49044T=CGMCC 4.7188T).
32375949	2	2	theme	strain	156:161	arg1	650086T					167:173	strain EGI 650086T	156:173	strain EGI 650086T	156:173	A novel endophytic actinobacterium, designated strain EGI 650086T, was isolated from the roots of Anabasis elatior (C.A.Mey.)
32375949	7	3	theme	sequence	674:681	arg1	similarities					683:694	the highest sequence similarities	662:694	the highest sequence similarities	662:694	Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes revealed that strain EGI 650086T formed a monophyletic clade within the genus Amycolatopsis and shared the highest sequence similarities with Amycolatopsis nigrescens JCM 14717T (97.1 %) and Amycolatopsis sacchari DSM 44468T (97.0 %).
32375949	18	4	theme	=KCTC	1975:1979	arg1	4.7188T					1994:2000	=KCTC 49044T=CGMCC 4.7188T	1975:2000	=KCTC 49044T=CGMCC 4.7188T	1975:2000	The type strain is EGI 650086T (=KCTC 49044T=CGMCC 4.7188T).
32375949	18	4	theme	=KCTC	1975:1979	arg1	650086T					1966:1972	EGI 650086T	1962:1972	EGI 650086T (=KCTC 49044T=CGMCC 4.7188T)	1962:2001	The type strain is EGI 650086T (=KCTC 49044T=CGMCC 4.7188T).
32375949	2	5	attach	isolated	180:187	arg2	actinobacterium					128:142	A novel endophytic actinobacterium	109:142	A novel endophytic actinobacterium	109:142	A novel endophytic actinobacterium, designated strain EGI 650086T, was isolated from the roots of Anabasis elatior (C.A.Mey.)
32375949	2	5	attach	isolated	180:187	arg1	roots					198:202	the roots	194:202	the roots of Anabasis elatior	194:222	A novel endophytic actinobacterium, designated strain EGI 650086T, was isolated from the roots of Anabasis elatior (C.A.Mey.)
32375949	14	6	dep	iso-C17 	1425:1432	arg1	anteiso-C17 					1446:1457	anteiso-C17 	1446:1457	anteiso-C17 	1446:1457	Major fatty acids were iso-C16 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
32375949	14	6	dep	iso-C17 	1425:1432	arg1	B					1462:1462	 1 B	1459:1462	iso-C17 : 1 I and/or anteiso-C17 : 1 B	1425:1462	Major fatty acids were iso-C16 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
32375949	14	6	dep	iso-C17 	1425:1432	arg1	I					1437:1437	 1 I	1434:1437	 1 I	1434:1437	Major fatty acids were iso-C16 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
32375949	16	7	theme	EGI	1812:1814	arg1	650086T					1816:1822	strain EGI 650086T	1805:1822	strain EGI 650086T	1805:1822	Polyphasic taxonomic characteristics indicated that strain EGI 650086T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis anabasis sp.
32375949	9	8	theme	average	905:911	arg1	identity					924:931	average nucleotide identity	905:931	average nucleotide identity	905:931	The average nucleotide identity and DNA-DNA hybridization values between strain EGI 650086T and the reference strains were 78.1-79.8 % and 22.1-23.0 %, respectively.
32375949	13	9	theme	predominant	1313:1323	arg1	menaquinones					1325:1336	The predominant menaquinones	1309:1336	The predominant menaquinones	1309:1336	The predominant menaquinones were MK-9 (H4) and MK-9 (H2).
32375949	13	9	theme	predominant	1313:1323	arg1	MK-9					1343:1346	MK-9	1343:1346	MK-9	1343:1346	The predominant menaquinones were MK-9 (H4) and MK-9 (H2).
32375949	5	10	theme	strain	316:321	arg1	position					300:307	The taxonomic position	286:307	The taxonomic position of the strain	286:321	The taxonomic position of the strain was investigated using a polyphasic taxonomic approach.
32375949	9	11	theme	identity	924:931	arg1	%					1050:1050	78.1-79.8 % and 22.1-23.0 %	1024:1050	78.1-79.8 % and 22.1-23.0 %	1024:1050	The average nucleotide identity and DNA-DNA hybridization values between strain EGI 650086T and the reference strains were 78.1-79.8 % and 22.1-23.0 %, respectively.
32375949	9	11	theme	identity	924:931	arg1	values					959:964	The average nucleotide identity and DNA-DNA hybridization values	901:964	The average nucleotide identity and DNA-DNA hybridization values between strain EGI 650086T and the reference strains	901:1017	The average nucleotide identity and DNA-DNA hybridization values between strain EGI 650086T and the reference strains were 78.1-79.8 % and 22.1-23.0 %, respectively.
32375949	16	12	theme	novel	1837:1841	arg1	species					1843:1849	a novel species	1835:1849	a novel species	1835:1849	Polyphasic taxonomic characteristics indicated that strain EGI 650086T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis anabasis sp.
32375949	9	13	theme	DNA-DNA	937:943	arg1	hybridization					945:957	DNA-DNA hybridization	937:957	DNA-DNA hybridization	937:957	The average nucleotide identity and DNA-DNA hybridization values between strain EGI 650086T and the reference strains were 78.1-79.8 % and 22.1-23.0 %, respectively.
32375949	7	14	theme	marker	546:551	arg1	genes					553:557	22 protein marker genes	535:557	22 protein marker genes	535:557	Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes revealed that strain EGI 650086T formed a monophyletic clade within the genus Amycolatopsis and shared the highest sequence similarities with Amycolatopsis nigrescens JCM 14717T (97.1 %) and Amycolatopsis sacchari DSM 44468T (97.0 %).
32375949	2	15	theme	elatior	216:222	arg1	roots					198:202	the roots	194:202	the roots of Anabasis elatior	194:222	A novel endophytic actinobacterium, designated strain EGI 650086T, was isolated from the roots of Anabasis elatior (C.A.Mey.)
32375949	5	16	theme	taxonomic	290:298	arg1	position					300:307	The taxonomic position	286:307	The taxonomic position of the strain	286:321	The taxonomic position of the strain was investigated using a polyphasic taxonomic approach.
32375949	8	17	with	similarities	803:814	arg1	strains					826:832	type strains	821:832	type strains of other species of the genus Amycolatopsis	821:876	Sequence similarities with type strains of other species of the genus Amycolatopsis were less than 97.0 %.
32375949	14	18	theme	 1	1459:1460	arg1	B					1462:1462	 1 B	1459:1462	iso-C17 : 1 I and/or anteiso-C17 : 1 B	1425:1462	Major fatty acids were iso-C16 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
32375949	9	19	theme	strain	974:979	arg1	650086T					985:991	strain EGI 650086T	974:991	strain EGI 650086T	974:991	The average nucleotide identity and DNA-DNA hybridization values between strain EGI 650086T and the reference strains were 78.1-79.8 % and 22.1-23.0 %, respectively.
32375949	16	20	theme	Polyphasic	1753:1762	arg1	characteristics					1774:1788	Polyphasic taxonomic characteristics	1753:1788	Polyphasic taxonomic characteristics	1753:1788	Polyphasic taxonomic characteristics indicated that strain EGI 650086T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis anabasis sp.
32375949	10	21	theme	70.1 mol	1139:1146	arg1	%					1147:1147	70.1 mol%	1139:1147	70.1 mol%	1139:1147	The genome of strain EGI 650086T was 10.9 Mb, with a DNA G+C content of 70.1 mol%.
32375949	7	22	theme	highest	666:672	arg1	similarities					683:694	the highest sequence similarities	662:694	the highest sequence similarities	662:694	Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes revealed that strain EGI 650086T formed a monophyletic clade within the genus Amycolatopsis and shared the highest sequence similarities with Amycolatopsis nigrescens JCM 14717T (97.1 %) and Amycolatopsis sacchari DSM 44468T (97.0 %).
32375949	15	23	theme	phosphatidylinositol	1649:1668	arg1	mannosides					1670:1679	phosphatidylinositol mannosides	1649:1679	phosphatidylinositol mannosides	1649:1679	The polar lipid profile of strain EGI 650086T included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, two unknown phospholipids, an unknown glycolipid and an unknown lipid.
32375949	7	24	theme	strain	573:578	arg1	650086T					584:590	strain EGI 650086T	573:590	strain EGI 650086T	573:590	Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes revealed that strain EGI 650086T formed a monophyletic clade within the genus Amycolatopsis and shared the highest sequence similarities with Amycolatopsis nigrescens JCM 14717T (97.1 %) and Amycolatopsis sacchari DSM 44468T (97.0 %).
32375949	7	25	theme	genes	553:557	arg1	concatenation					518:530	concatenation	518:530	concatenation of 22 protein marker genes	518:557	Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes revealed that strain EGI 650086T formed a monophyletic clade within the genus Amycolatopsis and shared the highest sequence similarities with Amycolatopsis nigrescens JCM 14717T (97.1 %) and Amycolatopsis sacchari DSM 44468T (97.0 %).
32375949	7	25	theme	genes	553:557	arg1	sequence					505:512	16S rRNA gene sequence	491:512	16S rRNA gene sequence	491:512	Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes revealed that strain EGI 650086T formed a monophyletic clade within the genus Amycolatopsis and shared the highest sequence similarities with Amycolatopsis nigrescens JCM 14717T (97.1 %) and Amycolatopsis sacchari DSM 44468T (97.0 %).
32375949	8	26	theme	type	821:824	arg1	strains					826:832	type strains	821:832	type strains of other species of the genus Amycolatopsis	821:876	Sequence similarities with type strains of other species of the genus Amycolatopsis were less than 97.0 %.
32375949	5	27	theme	taxonomic	359:367	arg1	approach					369:376	a polyphasic taxonomic approach	346:376	a polyphasic taxonomic approach	346:376	The taxonomic position of the strain was investigated using a polyphasic taxonomic approach.
32375949	9	28	theme	78.1-79.8 	1024:1033	arg1	%					1050:1050	78.1-79.8 % and 22.1-23.0 %	1024:1050	78.1-79.8 % and 22.1-23.0 %	1024:1050	The average nucleotide identity and DNA-DNA hybridization values between strain EGI 650086T and the reference strains were 78.1-79.8 % and 22.1-23.0 %, respectively.
32375949	9	28	theme	78.1-79.8 	1024:1033	arg1	values					959:964	The average nucleotide identity and DNA-DNA hybridization values	901:964	The average nucleotide identity and DNA-DNA hybridization values between strain EGI 650086T and the reference strains	901:1017	The average nucleotide identity and DNA-DNA hybridization values between strain EGI 650086T and the reference strains were 78.1-79.8 % and 22.1-23.0 %, respectively.
32375949	10	29	theme	DNA	1120:1122	arg1	content					1128:1134	a DNA G+C content	1118:1134	a DNA G+C content of 70.1 mol%	1118:1147	The genome of strain EGI 650086T was 10.9 Mb, with a DNA G+C content of 70.1 mol%.
32375949	7	30	theme	protein	538:544	arg1	genes					553:557	22 protein marker genes	535:557	22 protein marker genes	535:557	Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes revealed that strain EGI 650086T formed a monophyletic clade within the genus Amycolatopsis and shared the highest sequence similarities with Amycolatopsis nigrescens JCM 14717T (97.1 %) and Amycolatopsis sacchari DSM 44468T (97.0 %).
32375949	9	31	theme	 and	1035:1038	arg1	%					1050:1050	78.1-79.8 % and 22.1-23.0 %	1024:1050	78.1-79.8 % and 22.1-23.0 %	1024:1050	The average nucleotide identity and DNA-DNA hybridization values between strain EGI 650086T and the reference strains were 78.1-79.8 % and 22.1-23.0 %, respectively.
32375949	9	31	theme	 and	1035:1038	arg1	values					959:964	The average nucleotide identity and DNA-DNA hybridization values	901:964	The average nucleotide identity and DNA-DNA hybridization values between strain EGI 650086T and the reference strains	901:1017	The average nucleotide identity and DNA-DNA hybridization values between strain EGI 650086T and the reference strains were 78.1-79.8 % and 22.1-23.0 %, respectively.
32375949	0	32	theme	Amycolatopsis	0:12	arg1	sp					23:24	Amycolatopsis anabasis sp	0:24	Amycolatopsis anabasis sp.	0:25	Amycolatopsis anabasis sp.
32375949	7	33	theme	sacchari	764:771	arg1	44468T					777:782	Amycolatopsis sacchari DSM 44468T	750:782	Amycolatopsis sacchari DSM 44468T (97.0 %)	750:791	Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes revealed that strain EGI 650086T formed a monophyletic clade within the genus Amycolatopsis and shared the highest sequence similarities with Amycolatopsis nigrescens JCM 14717T (97.1 %) and Amycolatopsis sacchari DSM 44468T (97.0 %).
32375949	7	33	theme	sacchari	764:771	arg1	%					790:790	97.0 %	785:790	97.0 %	785:790	Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes revealed that strain EGI 650086T formed a monophyletic clade within the genus Amycolatopsis and shared the highest sequence similarities with Amycolatopsis nigrescens JCM 14717T (97.1 %) and Amycolatopsis sacchari DSM 44468T (97.0 %).
32375949	8	34	theme	species	843:849	arg1	strains					826:832	type strains	821:832	type strains of other species of the genus Amycolatopsis	821:876	Sequence similarities with type strains of other species of the genus Amycolatopsis were less than 97.0 %.
32375949	1	35	theme	novel	35:39	arg1	actinobacterium					52:66	a novel endophytic actinobacterium	33:66	a novel endophytic actinobacterium isolated from roots of Anabasis elatior	33:106	nov., a novel endophytic actinobacterium isolated from roots of Anabasis elatior.
32375949	15	36	theme	unknown	1712:1718	arg1	glycolipid					1720:1729	an unknown glycolipid	1709:1729	an unknown glycolipid	1709:1729	The polar lipid profile of strain EGI 650086T included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, two unknown phospholipids, an unknown glycolipid and an unknown lipid.
32375949	1	37	theme	elatior	100:106	arg1	roots					82:86	roots	82:86	roots of Anabasis elatior	82:106	nov., a novel endophytic actinobacterium isolated from roots of Anabasis elatior.
32375949	7	38	theme	gene	500:503	arg1	sequence					505:512	16S rRNA gene sequence	491:512	16S rRNA gene sequence	491:512	Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes revealed that strain EGI 650086T formed a monophyletic clade within the genus Amycolatopsis and shared the highest sequence similarities with Amycolatopsis nigrescens JCM 14717T (97.1 %) and Amycolatopsis sacchari DSM 44468T (97.0 %).
32375949	8	39	theme	Amycolatopsis	864:876	arg1	species					843:849	other species	837:849	other species of the genus Amycolatopsis	837:876	Sequence similarities with type strains of other species of the genus Amycolatopsis were less than 97.0 %.
32375949	6	40	theme	%	446:446	arg1	w/v					454:456	w/v	454:456	w/v	454:456	Growth occurred at 15-40 °C, pH 6.0-8.0 and in the presence of 0-6 % NaCl (w/v).
32375949	6	40	theme	%	446:446	arg1	 NaCl					447:451	0-6 % NaCl	442:451	0-6 % NaCl (w/v)	442:457	Growth occurred at 15-40 °C, pH 6.0-8.0 and in the presence of 0-6 % NaCl (w/v).
32375949	2	41	theme	EGI	163:165	arg1	650086T					167:173	strain EGI 650086T	156:173	strain EGI 650086T	156:173	A novel endophytic actinobacterium, designated strain EGI 650086T, was isolated from the roots of Anabasis elatior (C.A.Mey.)
32375949	15	42	theme	650086T	1504:1510	arg1	profile					1482:1488	The polar lipid profile	1466:1488	The polar lipid profile of strain EGI 650086T	1466:1510	The polar lipid profile of strain EGI 650086T included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, two unknown phospholipids, an unknown glycolipid and an unknown lipid.
32375949	10	43	theme	EGI	1088:1090	arg1	650086T					1092:1098	strain EGI 650086T	1081:1098	strain EGI 650086T	1081:1098	The genome of strain EGI 650086T was 10.9 Mb, with a DNA G+C content of 70.1 mol%.
32375949	7	44	theme	16S	491:493	arg1	sequence					505:512	16S rRNA gene sequence	491:512	16S rRNA gene sequence	491:512	Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes revealed that strain EGI 650086T formed a monophyletic clade within the genus Amycolatopsis and shared the highest sequence similarities with Amycolatopsis nigrescens JCM 14717T (97.1 %) and Amycolatopsis sacchari DSM 44468T (97.0 %).
32375949	9	45	theme	EGI	981:983	arg1	650086T					985:991	strain EGI 650086T	974:991	strain EGI 650086T	974:991	The average nucleotide identity and DNA-DNA hybridization values between strain EGI 650086T and the reference strains were 78.1-79.8 % and 22.1-23.0 %, respectively.
32375949	11	46	theme	diagnostic	1154:1163	arg1	acid					1173:1176	The diagnostic diamino acid	1150:1176	The diagnostic diamino acid in the peptidoglycan	1150:1197	The diagnostic diamino acid in the peptidoglycan was meso-diaminopimelic acid.
32375949	11	46	theme	diagnostic	1154:1163	arg1	acid					1223:1226	meso-diaminopimelic acid	1203:1226	meso-diaminopimelic acid	1203:1226	The diagnostic diamino acid in the peptidoglycan was meso-diaminopimelic acid.
32375949	15	47	theme	strain	1493:1498	arg1	650086T					1504:1510	strain EGI 650086T	1493:1510	strain EGI 650086T	1493:1510	The polar lipid profile of strain EGI 650086T included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, two unknown phospholipids, an unknown glycolipid and an unknown lipid.
32375949	7	48	theme	JCM	726:728	arg1	%					743:743	97.1 %	738:743	97.1 %	738:743	Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes revealed that strain EGI 650086T formed a monophyletic clade within the genus Amycolatopsis and shared the highest sequence similarities with Amycolatopsis nigrescens JCM 14717T (97.1 %) and Amycolatopsis sacchari DSM 44468T (97.0 %).
32375949	7	48	theme	JCM	726:728	arg1	14717T					730:735	Amycolatopsis nigrescens JCM 14717T	701:735	Amycolatopsis nigrescens JCM 14717T (97.1 %)	701:744	Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes revealed that strain EGI 650086T formed a monophyletic clade within the genus Amycolatopsis and shared the highest sequence similarities with Amycolatopsis nigrescens JCM 14717T (97.1 %) and Amycolatopsis sacchari DSM 44468T (97.0 %).
32375949	2	49	dep	isolated	180:187	arg1	C.A.Mey					225:231	C.A.Mey	225:231	C.A.Mey	225:231	A novel endophytic actinobacterium, designated strain EGI 650086T, was isolated from the roots of Anabasis elatior (C.A.Mey.)
32375949	11	50	from	acid	1173:1176	arg1	peptidoglycan					1185:1197	the peptidoglycan	1181:1197	the peptidoglycan	1181:1197	The diagnostic diamino acid in the peptidoglycan was meso-diaminopimelic acid.
32375949	18	51	theme	EGI	1962:1964	arg1	4.7188T					1994:2000	=KCTC 49044T=CGMCC 4.7188T	1975:2000	=KCTC 49044T=CGMCC 4.7188T	1975:2000	The type strain is EGI 650086T (=KCTC 49044T=CGMCC 4.7188T).
32375949	18	51	theme	EGI	1962:1964	arg1	strain					1952:1957	The type strain	1943:1957	The type strain	1943:1957	The type strain is EGI 650086T (=KCTC 49044T=CGMCC 4.7188T).
32375949	18	51	theme	EGI	1962:1964	arg1	650086T					1966:1972	EGI 650086T	1962:1972	EGI 650086T (=KCTC 49044T=CGMCC 4.7188T)	1962:2001	The type strain is EGI 650086T (=KCTC 49044T=CGMCC 4.7188T).
32375949	7	52	theme	Phylogenetic	460:471	arg1	analysis					473:480	Phylogenetic analysis	460:480	Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes	460:557	Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes revealed that strain EGI 650086T formed a monophyletic clade within the genus Amycolatopsis and shared the highest sequence similarities with Amycolatopsis nigrescens JCM 14717T (97.1 %) and Amycolatopsis sacchari DSM 44468T (97.0 %).
32375949	2	53	theme	novel	111:115	arg1	actinobacterium					128:142	A novel endophytic actinobacterium	109:142	A novel endophytic actinobacterium	109:142	A novel endophytic actinobacterium, designated strain EGI 650086T, was isolated from the roots of Anabasis elatior (C.A.Mey.)
32375949	15	54	theme	polar	1470:1474	arg1	profile					1482:1488	The polar lipid profile	1466:1488	The polar lipid profile of strain EGI 650086T	1466:1510	The polar lipid profile of strain EGI 650086T included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, two unknown phospholipids, an unknown glycolipid and an unknown lipid.
32375949	14	55	theme	Major	1368:1372	arg1	acids					1380:1384	Major fatty acids	1368:1384	Major fatty acids	1368:1384	Major fatty acids were iso-C16 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
32375949	10	56	theme	G+C	1124:1126	arg1	content					1128:1134	a DNA G+C content	1118:1134	a DNA G+C content of 70.1 mol%	1118:1147	The genome of strain EGI 650086T was 10.9 Mb, with a DNA G+C content of 70.1 mol%.
32375949	18	57	theme	49044T=CGMCC	1981:1992	arg1	4.7188T					1994:2000	=KCTC 49044T=CGMCC 4.7188T	1975:2000	=KCTC 49044T=CGMCC 4.7188T	1975:2000	The type strain is EGI 650086T (=KCTC 49044T=CGMCC 4.7188T).
32375949	18	57	theme	49044T=CGMCC	1981:1992	arg1	650086T					1966:1972	EGI 650086T	1962:1972	EGI 650086T (=KCTC 49044T=CGMCC 4.7188T)	1962:2001	The type strain is EGI 650086T (=KCTC 49044T=CGMCC 4.7188T).
32375949	14	58	dep	feature	1414:1420	arg1	iso-C17 					1425:1432	iso-C17 	1425:1432	iso-C17 : 1 I and/or anteiso-C17 : 1 B	1425:1462	Major fatty acids were iso-C16 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
32375949	15	59	theme	unknown	1686:1692	arg1	phospholipids					1694:1706	two unknown phospholipids	1682:1706	two unknown phospholipids	1682:1706	The polar lipid profile of strain EGI 650086T included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, two unknown phospholipids, an unknown glycolipid and an unknown lipid.
32375949	12	60	contain	contained	1257:1265	arg2	glucose					1289:1295	glucose	1289:1295	glucose	1289:1295	The major whole-cell sugars contained arabinose, galactose, glucose and ribose.
32375949	12	60	contain	contained	1257:1265	arg2	galactose					1278:1286	galactose	1278:1286	galactose	1278:1286	The major whole-cell sugars contained arabinose, galactose, glucose and ribose.
32375949	12	60	contain	contained	1257:1265	arg2	arabinose					1267:1275	arabinose	1267:1275	arabinose	1267:1275	The major whole-cell sugars contained arabinose, galactose, glucose and ribose.
32375949	12	60	contain	contained	1257:1265	arg1	sugars					1250:1255	The major whole-cell sugars	1229:1255	The major whole-cell sugars	1229:1255	The major whole-cell sugars contained arabinose, galactose, glucose and ribose.
32375949	12	60	contain	contained	1257:1265	arg2	ribose					1301:1306	ribose	1301:1306	ribose	1301:1306	The major whole-cell sugars contained arabinose, galactose, glucose and ribose.
32375949	9	61	theme	nucleotide	913:922	arg1	identity					924:931	average nucleotide identity	905:931	average nucleotide identity	905:931	The average nucleotide identity and DNA-DNA hybridization values between strain EGI 650086T and the reference strains were 78.1-79.8 % and 22.1-23.0 %, respectively.
32375949	16	62	theme	Amycolatopsis	1864:1876	arg1	species					1843:1849	a novel species	1835:1849	a novel species	1835:1849	Polyphasic taxonomic characteristics indicated that strain EGI 650086T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis anabasis sp.
32375949	1	63	attach	isolated	68:75	arg1	roots					82:86	roots	82:86	roots of Anabasis elatior	82:106	nov., a novel endophytic actinobacterium isolated from roots of Anabasis elatior.
32375949	1	63	attach	isolated	68:75	arg2	actinobacterium					52:66	a novel endophytic actinobacterium	33:66	a novel endophytic actinobacterium isolated from roots of Anabasis elatior	33:106	nov., a novel endophytic actinobacterium isolated from roots of Anabasis elatior.
32375949	9	64	theme	hybridization	945:957	arg1	%					1050:1050	78.1-79.8 % and 22.1-23.0 %	1024:1050	78.1-79.8 % and 22.1-23.0 %	1024:1050	The average nucleotide identity and DNA-DNA hybridization values between strain EGI 650086T and the reference strains were 78.1-79.8 % and 22.1-23.0 %, respectively.
32375949	9	64	theme	hybridization	945:957	arg1	values					959:964	The average nucleotide identity and DNA-DNA hybridization values	901:964	The average nucleotide identity and DNA-DNA hybridization values between strain EGI 650086T and the reference strains	901:1017	The average nucleotide identity and DNA-DNA hybridization values between strain EGI 650086T and the reference strains were 78.1-79.8 % and 22.1-23.0 %, respectively.
32375949	2	65	theme	Anabasis	207:214	arg1	elatior					216:222	Anabasis elatior	207:222	Anabasis elatior	207:222	A novel endophytic actinobacterium, designated strain EGI 650086T, was isolated from the roots of Anabasis elatior (C.A.Mey.)
32375949	7	66	theme	genus	631:635	arg1	Amycolatopsis					637:649	the genus Amycolatopsis	627:649	the genus Amycolatopsis	627:649	Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes revealed that strain EGI 650086T formed a monophyletic clade within the genus Amycolatopsis and shared the highest sequence similarities with Amycolatopsis nigrescens JCM 14717T (97.1 %) and Amycolatopsis sacchari DSM 44468T (97.0 %).
32375949	16	67	theme	taxonomic	1764:1772	arg1	characteristics					1774:1788	Polyphasic taxonomic characteristics	1753:1788	Polyphasic taxonomic characteristics	1753:1788	Polyphasic taxonomic characteristics indicated that strain EGI 650086T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis anabasis sp.
32375949	12	68	theme	whole-cell	1239:1248	arg1	sugars					1250:1255	The major whole-cell sugars	1229:1255	The major whole-cell sugars	1229:1255	The major whole-cell sugars contained arabinose, galactose, glucose and ribose.
32375949	1	69	dep	actinobacterium	52:66	arg1	nov.					27:30	nov.	27:30	nov.	27:30	nov., a novel endophytic actinobacterium isolated from roots of Anabasis elatior.
32375949	10	70	theme	%	1147:1147	arg1	content					1128:1134	a DNA G+C content	1118:1134	a DNA G+C content of 70.1 mol%	1118:1147	The genome of strain EGI 650086T was 10.9 Mb, with a DNA G+C content of 70.1 mol%.
32375949	16	71	theme	name	1893:1896	arg1	sp					1921:1922	the name Amycolatopsis anabasis sp	1889:1922	the name Amycolatopsis anabasis sp	1889:1922	Polyphasic taxonomic characteristics indicated that strain EGI 650086T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis anabasis sp.
32375949	14	72	dep	iso-C16 	1391:1398	arg1	feature					1414:1420	summed feature 4	1407:1422	summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B)	1407:1463	Major fatty acids were iso-C16 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
32375949	14	72	dep	iso-C16 	1391:1398	arg1	 0					1400:1401	 0	1400:1401	 0	1400:1401	Major fatty acids were iso-C16 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
32375949	7	73	theme	monophyletic	601:612	arg1	clade					614:618	a monophyletic clade	599:618	a monophyletic clade	599:618	Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes revealed that strain EGI 650086T formed a monophyletic clade within the genus Amycolatopsis and shared the highest sequence similarities with Amycolatopsis nigrescens JCM 14717T (97.1 %) and Amycolatopsis sacchari DSM 44468T (97.0 %).
32375949	16	74	theme	strain	1805:1810	arg1	650086T					1816:1822	strain EGI 650086T	1805:1822	strain EGI 650086T	1805:1822	Polyphasic taxonomic characteristics indicated that strain EGI 650086T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis anabasis sp.
32375949	11	75	theme	meso-diaminopimelic	1203:1221	arg1	acid					1173:1176	The diagnostic diamino acid	1150:1176	The diagnostic diamino acid in the peptidoglycan	1150:1197	The diagnostic diamino acid in the peptidoglycan was meso-diaminopimelic acid.
32375949	11	75	theme	meso-diaminopimelic	1203:1221	arg1	acid					1223:1226	meso-diaminopimelic acid	1203:1226	meso-diaminopimelic acid	1203:1226	The diagnostic diamino acid in the peptidoglycan was meso-diaminopimelic acid.
32375949	14	76	theme	 1	1434:1435	arg1	I					1437:1437	 1 I	1434:1437	 1 I	1434:1437	Major fatty acids were iso-C16 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
32375949	9	77	theme	reference	1001:1009	arg1	strains					1011:1017	the reference strains	997:1017	the reference strains	997:1017	The average nucleotide identity and DNA-DNA hybridization values between strain EGI 650086T and the reference strains were 78.1-79.8 % and 22.1-23.0 %, respectively.
32375949	1	78	theme	endophytic	41:50	arg1	actinobacterium					52:66	a novel endophytic actinobacterium	33:66	a novel endophytic actinobacterium isolated from roots of Anabasis elatior	33:106	nov., a novel endophytic actinobacterium isolated from roots of Anabasis elatior.
32375949	8	79	theme	Sequence	794:801	arg1	similarities					803:814	Sequence similarities	794:814	Sequence similarities with type strains of other species of the genus Amycolatopsis	794:876	Sequence similarities with type strains of other species of the genus Amycolatopsis were less than 97.0 %.
32375949	5	80	theme	polyphasic	348:357	arg1	approach					369:376	a polyphasic taxonomic approach	346:376	a polyphasic taxonomic approach	346:376	The taxonomic position of the strain was investigated using a polyphasic taxonomic approach.
32375949	16	81	theme	anabasis	1912:1919	arg1	sp					1921:1922	the name Amycolatopsis anabasis sp	1889:1922	the name Amycolatopsis anabasis sp	1889:1922	Polyphasic taxonomic characteristics indicated that strain EGI 650086T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis anabasis sp.
32375949	10	82	with	10.9 Mb	1104:1110	arg1	content					1128:1134	a DNA G+C content	1118:1134	a DNA G+C content of 70.1 mol%	1118:1147	The genome of strain EGI 650086T was 10.9 Mb, with a DNA G+C content of 70.1 mol%.
32375949	7	83	theme	EGI	580:582	arg1	650086T					584:590	strain EGI 650086T	573:590	strain EGI 650086T	573:590	Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes revealed that strain EGI 650086T formed a monophyletic clade within the genus Amycolatopsis and shared the highest sequence similarities with Amycolatopsis nigrescens JCM 14717T (97.1 %) and Amycolatopsis sacchari DSM 44468T (97.0 %).
32375949	9	84	theme	%	1034:1034	arg1	%					1050:1050	78.1-79.8 % and 22.1-23.0 %	1024:1050	78.1-79.8 % and 22.1-23.0 %	1024:1050	The average nucleotide identity and DNA-DNA hybridization values between strain EGI 650086T and the reference strains were 78.1-79.8 % and 22.1-23.0 %, respectively.
32375949	9	84	theme	%	1034:1034	arg1	values					959:964	The average nucleotide identity and DNA-DNA hybridization values	901:964	The average nucleotide identity and DNA-DNA hybridization values between strain EGI 650086T and the reference strains	901:1017	The average nucleotide identity and DNA-DNA hybridization values between strain EGI 650086T and the reference strains were 78.1-79.8 % and 22.1-23.0 %, respectively.
32375949	0	85	theme	anabasis	14:21	arg1	sp					23:24	Amycolatopsis anabasis sp	0:24	Amycolatopsis anabasis sp.	0:25	Amycolatopsis anabasis sp.
32375949	12	86	theme	major	1233:1237	arg1	sugars					1250:1255	The major whole-cell sugars	1229:1255	The major whole-cell sugars	1229:1255	The major whole-cell sugars contained arabinose, galactose, glucose and ribose.
32375949	14	87	theme	summed	1407:1412	arg1	feature					1414:1420	summed feature 4	1407:1422	summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B)	1407:1463	Major fatty acids were iso-C16 : 0 and summed feature 4 (iso-C17 : 1 I and/or anteiso-C17 : 1 B).
32375949	7	88	theme	DSM	773:775	arg1	44468T					777:782	Amycolatopsis sacchari DSM 44468T	750:782	Amycolatopsis sacchari DSM 44468T (97.0 %)	750:791	Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes revealed that strain EGI 650086T formed a monophyletic clade within the genus Amycolatopsis and shared the highest sequence similarities with Amycolatopsis nigrescens JCM 14717T (97.1 %) and Amycolatopsis sacchari DSM 44468T (97.0 %).
32375949	7	88	theme	DSM	773:775	arg1	%					790:790	97.0 %	785:790	97.0 %	785:790	Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes revealed that strain EGI 650086T formed a monophyletic clade within the genus Amycolatopsis and shared the highest sequence similarities with Amycolatopsis nigrescens JCM 14717T (97.1 %) and Amycolatopsis sacchari DSM 44468T (97.0 %).
32375949	1	89	theme	Anabasis	91:98	arg1	elatior					100:106	Anabasis elatior	91:106	Anabasis elatior	91:106	nov., a novel endophytic actinobacterium isolated from roots of Anabasis elatior.
32375949	8	90	theme	other	837:841	arg1	species					843:849	other species	837:849	other species of the genus Amycolatopsis	837:876	Sequence similarities with type strains of other species of the genus Amycolatopsis were less than 97.0 %.
32375949	7	91	theme	Amycolatopsis	750:762	arg1	44468T					777:782	Amycolatopsis sacchari DSM 44468T	750:782	Amycolatopsis sacchari DSM 44468T (97.0 %)	750:791	Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes revealed that strain EGI 650086T formed a monophyletic clade within the genus Amycolatopsis and shared the highest sequence similarities with Amycolatopsis nigrescens JCM 14717T (97.1 %) and Amycolatopsis sacchari DSM 44468T (97.0 %).
32375949	7	91	theme	Amycolatopsis	750:762	arg1	%					790:790	97.0 %	785:790	97.0 %	785:790	Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes revealed that strain EGI 650086T formed a monophyletic clade within the genus Amycolatopsis and shared the highest sequence similarities with Amycolatopsis nigrescens JCM 14717T (97.1 %) and Amycolatopsis sacchari DSM 44468T (97.0 %).
32375949	7	92	theme	rRNA	495:498	arg1	sequence					505:512	16S rRNA gene sequence	491:512	16S rRNA gene sequence	491:512	Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes revealed that strain EGI 650086T formed a monophyletic clade within the genus Amycolatopsis and shared the highest sequence similarities with Amycolatopsis nigrescens JCM 14717T (97.1 %) and Amycolatopsis sacchari DSM 44468T (97.0 %).
32375949	6	93	theme	 NaCl	447:451	arg1	presence					430:437	the presence	426:437	the presence of 0-6 % NaCl (w/v)	426:457	Growth occurred at 15-40 °C, pH 6.0-8.0 and in the presence of 0-6 % NaCl (w/v).
32375949	7	94	theme	Amycolatopsis	701:713	arg1	%					743:743	97.1 %	738:743	97.1 %	738:743	Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes revealed that strain EGI 650086T formed a monophyletic clade within the genus Amycolatopsis and shared the highest sequence similarities with Amycolatopsis nigrescens JCM 14717T (97.1 %) and Amycolatopsis sacchari DSM 44468T (97.0 %).
32375949	7	94	theme	Amycolatopsis	701:713	arg1	14717T					730:735	Amycolatopsis nigrescens JCM 14717T	701:735	Amycolatopsis nigrescens JCM 14717T (97.1 %)	701:744	Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes revealed that strain EGI 650086T formed a monophyletic clade within the genus Amycolatopsis and shared the highest sequence similarities with Amycolatopsis nigrescens JCM 14717T (97.1 %) and Amycolatopsis sacchari DSM 44468T (97.0 %).
32375949	15	95	theme	EGI	1500:1502	arg1	650086T					1504:1510	strain EGI 650086T	1493:1510	strain EGI 650086T	1493:1510	The polar lipid profile of strain EGI 650086T included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, two unknown phospholipids, an unknown glycolipid and an unknown lipid.
32375949	10	96	theme	650086T	1092:1098	arg1	genome					1071:1076	The genome	1067:1076	The genome of strain EGI 650086T	1067:1098	The genome of strain EGI 650086T was 10.9 Mb, with a DNA G+C content of 70.1 mol%.
32375949	10	96	theme	650086T	1092:1098	arg1	10.9 Mb					1104:1110	10.9 Mb	1104:1110	10.9 Mb	1104:1110	The genome of strain EGI 650086T was 10.9 Mb, with a DNA G+C content of 70.1 mol%.
32375949	8	97	theme	genus	858:862	arg1	Amycolatopsis					864:876	the genus Amycolatopsis	854:876	the genus Amycolatopsis	854:876	Sequence similarities with type strains of other species of the genus Amycolatopsis were less than 97.0 %.
32375949	16	98	theme	genus	1858:1862	arg1	Amycolatopsis					1864:1876	the genus Amycolatopsis	1854:1876	the genus Amycolatopsis	1854:1876	Polyphasic taxonomic characteristics indicated that strain EGI 650086T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis anabasis sp.
32375949	6	99	theme	0-6 	442:445	arg1	w/v					454:456	w/v	454:456	w/v	454:456	Growth occurred at 15-40 °C, pH 6.0-8.0 and in the presence of 0-6 % NaCl (w/v).
32375949	6	99	theme	0-6 	442:445	arg1	 NaCl					447:451	0-6 % NaCl	442:451	0-6 % NaCl (w/v)	442:457	Growth occurred at 15-40 °C, pH 6.0-8.0 and in the presence of 0-6 % NaCl (w/v).
32375949	10	100	theme	strain	1081:1086	arg1	650086T					1092:1098	strain EGI 650086T	1081:1098	strain EGI 650086T	1081:1098	The genome of strain EGI 650086T was 10.9 Mb, with a DNA G+C content of 70.1 mol%.
32375949	11	101	theme	diamino	1165:1171	arg1	acid					1173:1176	The diagnostic diamino acid	1150:1176	The diagnostic diamino acid in the peptidoglycan	1150:1197	The diagnostic diamino acid in the peptidoglycan was meso-diaminopimelic acid.
32375949	11	101	theme	diamino	1165:1171	arg1	acid					1223:1226	meso-diaminopimelic acid	1203:1226	meso-diaminopimelic acid	1203:1226	The diagnostic diamino acid in the peptidoglycan was meso-diaminopimelic acid.
32375949	15	102	theme	lipid	1476:1480	arg1	profile					1482:1488	The polar lipid profile	1466:1488	The polar lipid profile of strain EGI 650086T	1466:1510	The polar lipid profile of strain EGI 650086T included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, two unknown phospholipids, an unknown glycolipid and an unknown lipid.
32375949	16	103	theme	Amycolatopsis	1898:1910	arg1	sp					1921:1922	the name Amycolatopsis anabasis sp	1889:1922	the name Amycolatopsis anabasis sp	1889:1922	Polyphasic taxonomic characteristics indicated that strain EGI 650086T represents a novel species of the genus Amycolatopsis, for which the name Amycolatopsis anabasis sp.
32375949	15	104	theme	unknown	1738:1744	arg1	lipid					1746:1750	an unknown lipid	1735:1750	an unknown lipid	1735:1750	The polar lipid profile of strain EGI 650086T included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannosides, two unknown phospholipids, an unknown glycolipid and an unknown lipid.
32375949	2	105	theme	endophytic	117:126	arg1	actinobacterium					128:142	A novel endophytic actinobacterium	109:142	A novel endophytic actinobacterium	109:142	A novel endophytic actinobacterium, designated strain EGI 650086T, was isolated from the roots of Anabasis elatior (C.A.Mey.)
32375949	7	106	theme	nigrescens	715:724	arg1	%					743:743	97.1 %	738:743	97.1 %	738:743	Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes revealed that strain EGI 650086T formed a monophyletic clade within the genus Amycolatopsis and shared the highest sequence similarities with Amycolatopsis nigrescens JCM 14717T (97.1 %) and Amycolatopsis sacchari DSM 44468T (97.0 %).
32375949	7	106	theme	nigrescens	715:724	arg1	14717T					730:735	Amycolatopsis nigrescens JCM 14717T	701:735	Amycolatopsis nigrescens JCM 14717T (97.1 %)	701:744	Phylogenetic analysis based on 16S rRNA gene sequence and concatenation of 22 protein marker genes revealed that strain EGI 650086T formed a monophyletic clade within the genus Amycolatopsis and shared the highest sequence similarities with Amycolatopsis nigrescens JCM 14717T (97.1 %) and Amycolatopsis sacchari DSM 44468T (97.0 %).
32071270	9	0	theme	periplasmic	1881:1891	arg1	systems					1959:1965	low-molecular-weight dissolved organic carbon (DOC) through ATP-binding cassette (ABC), tripartite ATP-independent periplasmic (TRAP), and tripartite tricarboxylate transporter (TTT) transport systems	1766:1965	low-molecular-weight dissolved organic carbon (DOC) through ATP-binding cassette (ABC), tripartite ATP-independent periplasmic (TRAP), and tripartite tricarboxylate transporter (TTT) transport systems	1766:1965	population mainly utilized low-molecular-weight dissolved organic carbon (DOC) through ATP-binding cassette (ABC), tripartite ATP-independent periplasmic (TRAP), and tripartite tricarboxylate transporter (TTT) transport systems.
32071270	6	1	theme	flavobacterial	1254:1267	arg1	populations					1269:1279	the flavobacterial populations	1250:1279	the flavobacterial populations	1250:1279	Two of the flavobacterial populations, Muricauda and Winogradskyella, and an SM1A02 population, displayed preferences for initial degradation of complex compounds and biopolymers, as evinced by high abundances of TonB-dependent transporters (TBDTs), glycoside hydrolase, and peptidase proteins.
32071270	11	2	dep	in	2382:2383	arg1	situ					2385:2388	situ	2385:2388	situ	2385:2388	In addition to nutrient exchange, removal of reactive oxygen species and vitamin trafficking might also contribute to the maintenance of the Synechococcus-heterotroph coculture system and the interactions shaping the system.IMPORTANCE The high complexity of in situ ecosystems renders it difficult to study marine microbial photoautotroph-heterotroph interactions.
32071270	6	3	theme	TonB-dependent	1456:1469	arg1	TBDTs					1485:1489	TBDTs	1485:1489	TBDTs	1485:1489	Two of the flavobacterial populations, Muricauda and Winogradskyella, and an SM1A02 population, displayed preferences for initial degradation of complex compounds and biopolymers, as evinced by high abundances of TonB-dependent transporters (TBDTs), glycoside hydrolase, and peptidase proteins.
32071270	6	3	theme	TonB-dependent	1456:1469	arg1	transporters					1471:1482	TonB-dependent transporters	1456:1482	TonB-dependent transporters (TBDTs)	1456:1490	Two of the flavobacterial populations, Muricauda and Winogradskyella, and an SM1A02 population, displayed preferences for initial degradation of complex compounds and biopolymers, as evinced by high abundances of TonB-dependent transporters (TBDTs), glycoside hydrolase, and peptidase proteins.
32071270	2	4	theme	ocean	419:423	arg1	ecotype					439:445	an open ocean Synechococcus ecotype	411:445	an open ocean Synechococcus ecotype	411:445	Here, bacterial community composition, lifestyle preference, and genomic- and proteomic-level metabolic characteristics were investigated for an open ocean Synechococcus ecotype and its associated heterotrophs over 91 days of cocultivation.
32071270	14	5	theme	populations	2898:2908	arg1	potentials					2839:2848	potentials	2839:2848	potentials	2839:2848	In the present study, combined metagenomic and metaproteomic data supplied the metabolic potentials and activities of uncultured dominant bacterial populations in the coculture system.
32071270	14	5	theme	populations	2898:2908	arg1	activities					2854:2863	activities	2854:2863	activities	2854:2863	In the present study, combined metagenomic and metaproteomic data supplied the metabolic potentials and activities of uncultured dominant bacterial populations in the coculture system.
32071270	10	6	theme	organic	2079:2085	arg1	matter					2087:2092	Synechococcus-derived organic matter	2057:2092	Synechococcus-derived organic matter	2057:2092	The heterotrophic bacterial populations exhibited complementary mechanisms for degrading Synechococcus-derived organic matter and driving nutrient cycling.
32071270	14	7	theme	combined	2772:2779	arg1	data					2811:2814	combined metagenomic and metaproteomic data	2772:2814	combined metagenomic and metaproteomic data	2772:2814	In the present study, combined metagenomic and metaproteomic data supplied the metabolic potentials and activities of uncultured dominant bacterial populations in the coculture system.
32071270	2	8	theme	genomic-	334:341	arg1	characteristics					373:387	genomic- and proteomic-level metabolic characteristics	334:387	genomic- and proteomic-level metabolic characteristics	334:387	Here, bacterial community composition, lifestyle preference, and genomic- and proteomic-level metabolic characteristics were investigated for an open ocean Synechococcus ecotype and its associated heterotrophs over 91 days of cocultivation.
32071270	14	9	theme	dominant	2879:2886	arg1	populations					2898:2908	uncultured dominant bacterial populations	2868:2908	uncultured dominant bacterial populations	2868:2908	In the present study, combined metagenomic and metaproteomic data supplied the metabolic potentials and activities of uncultured dominant bacterial populations in the coculture system.
32071270	4	10	theme	dominant	984:991	arg1	bacteria					1007:1014	the five dominant heterotrophic bacteria	975:1014	the five dominant heterotrophic bacteria	975:1014	Six high-quality genomes, including Synechococcus and the five dominant heterotrophic bacteria, were reconstructed.
32071270	11	11	theme	reactive	2169:2176	arg1	species					2185:2191	reactive oxygen species	2169:2191	reactive oxygen species	2169:2191	In addition to nutrient exchange, removal of reactive oxygen species and vitamin trafficking might also contribute to the maintenance of the Synechococcus-heterotroph coculture system and the interactions shaping the system.IMPORTANCE The high complexity of in situ ecosystems renders it difficult to study marine microbial photoautotroph-heterotroph interactions.
32071270	10	12	theme	heterotrophic	1972:1984	arg1	populations					1996:2006	The heterotrophic bacterial populations	1968:2006	The heterotrophic bacterial populations	1968:2006	The heterotrophic bacterial populations exhibited complementary mechanisms for degrading Synechococcus-derived organic matter and driving nutrient cycling.
32071270	13	13	theme	photoautotrophic	2722:2737	arg1	organisms					2739:2747	single photoautotrophic organisms	2715:2747	single photoautotrophic organisms	2715:2747	However, in situ interactions comprise far more diverse heterotrophic bacterial associations with single photoautotrophic organisms.
32071270	9	14	theme	tripartite	1905:1914	arg1	TTT					1944:1946	TTT	1944:1946	TTT	1944:1946	population mainly utilized low-molecular-weight dissolved organic carbon (DOC) through ATP-binding cassette (ABC), tripartite ATP-independent periplasmic (TRAP), and tripartite tricarboxylate transporter (TTT) transport systems.
32071270	9	14	theme	tripartite	1905:1914	arg1	transporter					1931:1941	tripartite tricarboxylate transporter	1905:1941	tripartite tricarboxylate transporter (TTT)	1905:1947	population mainly utilized low-molecular-weight dissolved organic carbon (DOC) through ATP-binding cassette (ABC), tripartite ATP-independent periplasmic (TRAP), and tripartite tricarboxylate transporter (TTT) transport systems.
32071270	9	15	theme	transporter	1931:1941	arg1	systems					1959:1965	low-molecular-weight dissolved organic carbon (DOC) through ATP-binding cassette (ABC), tripartite ATP-independent periplasmic (TRAP), and tripartite tricarboxylate transporter (TTT) transport systems	1766:1965	low-molecular-weight dissolved organic carbon (DOC) through ATP-binding cassette (ABC), tripartite ATP-independent periplasmic (TRAP), and tripartite tricarboxylate transporter (TTT) transport systems	1766:1965	population mainly utilized low-molecular-weight dissolved organic carbon (DOC) through ATP-binding cassette (ABC), tripartite ATP-independent periplasmic (TRAP), and tripartite tricarboxylate transporter (TTT) transport systems.
32071270	11	16	theme	microbial	2438:2446	arg1	interactions					2475:2486	marine microbial photoautotroph-heterotroph interactions	2431:2486	marine microbial photoautotroph-heterotroph interactions	2431:2486	In addition to nutrient exchange, removal of reactive oxygen species and vitamin trafficking might also contribute to the maintenance of the Synechococcus-heterotroph coculture system and the interactions shaping the system.IMPORTANCE The high complexity of in situ ecosystems renders it difficult to study marine microbial photoautotroph-heterotroph interactions.
32071270	2	17	theme	proteomic-level	347:361	arg1	characteristics					373:387	genomic- and proteomic-level metabolic characteristics	334:387	genomic- and proteomic-level metabolic characteristics	334:387	Here, bacterial community composition, lifestyle preference, and genomic- and proteomic-level metabolic characteristics were investigated for an open ocean Synechococcus ecotype and its associated heterotrophs over 91 days of cocultivation.
32071270	1	18	theme	ocean	249:253	arg1	environments					255:266	upper ocean environments	243:266	upper ocean environments	243:266	Microbial photoautotroph-heterotroph interactions underlie marine food webs and shape ecosystem diversity and structure in upper ocean environments.
32071270	11	19	theme	high	2363:2366	arg1	complexity					2368:2377	The high complexity	2359:2377	The high complexity of in situ ecosystems	2359:2399	In addition to nutrient exchange, removal of reactive oxygen species and vitamin trafficking might also contribute to the maintenance of the Synechococcus-heterotroph coculture system and the interactions shaping the system.IMPORTANCE The high complexity of in situ ecosystems renders it difficult to study marine microbial photoautotroph-heterotroph interactions.
32071270	7	20	theme	present	1570:1576	arg1	loci					1565:1568	Polysaccharide utilization loci	1538:1568	Polysaccharide utilization loci present in the flavobacterial genomes	1538:1606	Polysaccharide utilization loci present in the flavobacterial genomes influence their lifestyle preferences and close associations with phytoplankton.
32071270	11	21	theme	oxygen	2178:2183	arg1	species					2185:2191	reactive oxygen species	2169:2191	reactive oxygen species	2169:2191	In addition to nutrient exchange, removal of reactive oxygen species and vitamin trafficking might also contribute to the maintenance of the Synechococcus-heterotroph coculture system and the interactions shaping the system.IMPORTANCE The high complexity of in situ ecosystems renders it difficult to study marine microbial photoautotroph-heterotroph interactions.
32071270	6	22	theme	glycoside	1493:1501	arg1	hydrolase					1503:1511	glycoside hydrolase	1493:1511	glycoside hydrolase	1493:1511	Two of the flavobacterial populations, Muricauda and Winogradskyella, and an SM1A02 population, displayed preferences for initial degradation of complex compounds and biopolymers, as evinced by high abundances of TonB-dependent transporters (TBDTs), glycoside hydrolase, and peptidase proteins.
32071270	2	23	theme	bacterial	275:283	arg1	composition					295:305	bacterial community composition	275:305	bacterial community composition	275:305	Here, bacterial community composition, lifestyle preference, and genomic- and proteomic-level metabolic characteristics were investigated for an open ocean Synechococcus ecotype and its associated heterotrophs over 91 days of cocultivation.
32071270	9	24	theme	transport	1949:1957	arg1	systems					1959:1965	low-molecular-weight dissolved organic carbon (DOC) through ATP-binding cassette (ABC), tripartite ATP-independent periplasmic (TRAP), and tripartite tricarboxylate transporter (TTT) transport systems	1766:1965	low-molecular-weight dissolved organic carbon (DOC) through ATP-binding cassette (ABC), tripartite ATP-independent periplasmic (TRAP), and tripartite tricarboxylate transporter (TTT) transport systems	1766:1965	population mainly utilized low-molecular-weight dissolved organic carbon (DOC) through ATP-binding cassette (ABC), tripartite ATP-independent periplasmic (TRAP), and tripartite tricarboxylate transporter (TTT) transport systems.
32071270	0	25	from	Interactions	79:90	arg1	Culture					111:117	a Synechococcus Culture	95:117	a Synechococcus Culture	95:117	Metagenomic and Metaproteomic Insights into Photoautotrophic and Heterotrophic Interactions in a Synechococcus Culture.
32071270	7	26	theme	utilization	1553:1563	arg1	loci					1565:1568	Polysaccharide utilization loci	1538:1568	Polysaccharide utilization loci present in the flavobacterial genomes	1538:1606	Polysaccharide utilization loci present in the flavobacterial genomes influence their lifestyle preferences and close associations with phytoplankton.
32071270	3	27	theme	Synechococcus	892:904	arg1	phases					913:918	Synechococcus growth phases	892:918	Synechococcus growth phases	892:918	The associated heterotrophic bacterial assembly mostly constituted five classes, including Flavobacteria, Bacteroidetes, Phycisphaerae, Gammaproteobacteria, and Alphaproteobacteria The seven most abundant taxa/genera comprised >90% of the total heterotrophic bacterial community, and five of these displayed distinct lifestyle preferences (free-living or attached) and responses to Synechococcus growth phases.
32071270	5	28	theme	organic	1202:1208	arg1	biosynthesis					1217:1228	organic matter biosynthesis	1202:1228	organic matter biosynthesis	1202:1228	The only primary producer of the coculture system, Synechococcus, displayed metabolic processes primarily involved in inorganic nutrient uptake, photosynthesis, and organic matter biosynthesis and release.
32071270	10	29	theme	Synechococcus-derived	2057:2077	arg1	matter					2087:2092	Synechococcus-derived organic matter	2057:2092	Synechococcus-derived organic matter	2057:2092	The heterotrophic bacterial populations exhibited complementary mechanisms for degrading Synechococcus-derived organic matter and driving nutrient cycling.
32071270	3	30	dep	Gammaproteobacteria	646:664	arg1	Phycisphaerae					631:643	Phycisphaerae	631:643	Phycisphaerae	631:643	The associated heterotrophic bacterial assembly mostly constituted five classes, including Flavobacteria, Bacteroidetes, Phycisphaerae, Gammaproteobacteria, and Alphaproteobacteria The seven most abundant taxa/genera comprised >90% of the total heterotrophic bacterial community, and five of these displayed distinct lifestyle preferences (free-living or attached) and responses to Synechococcus growth phases.
32071270	9	31	used	utilized	1757:1764	arg2	population					1739:1748	population	1739:1748	population	1739:1748	population mainly utilized low-molecular-weight dissolved organic carbon (DOC) through ATP-binding cassette (ABC), tripartite ATP-independent periplasmic (TRAP), and tripartite tricarboxylate transporter (TTT) transport systems.
32071270	6	32	theme	complex	1388:1394	arg1	compounds					1396:1404	complex compounds	1388:1404	complex compounds	1388:1404	Two of the flavobacterial populations, Muricauda and Winogradskyella, and an SM1A02 population, displayed preferences for initial degradation of complex compounds and biopolymers, as evinced by high abundances of TonB-dependent transporters (TBDTs), glycoside hydrolase, and peptidase proteins.
32071270	3	33	theme	total	749:753	arg1	community					779:787	the total heterotrophic bacterial community	745:787	the total heterotrophic bacterial community	745:787	The associated heterotrophic bacterial assembly mostly constituted five classes, including Flavobacteria, Bacteroidetes, Phycisphaerae, Gammaproteobacteria, and Alphaproteobacteria The seven most abundant taxa/genera comprised >90% of the total heterotrophic bacterial community, and five of these displayed distinct lifestyle preferences (free-living or attached) and responses to Synechococcus growth phases.
32071270	1	34	from	structure	230:238	arg1	environments					255:266	upper ocean environments	243:266	upper ocean environments	243:266	Microbial photoautotroph-heterotroph interactions underlie marine food webs and shape ecosystem diversity and structure in upper ocean environments.
32071270	5	35	theme	system	1080:1085	arg1	producer					1054:1061	The only primary producer	1037:1061	The only primary producer of the coculture system	1037:1085	The only primary producer of the coculture system, Synechococcus, displayed metabolic processes primarily involved in inorganic nutrient uptake, photosynthesis, and organic matter biosynthesis and release.
32071270	5	35	theme	system	1080:1085	arg1	Synechococcus					1088:1100	Synechococcus	1088:1100	Synechococcus	1088:1100	The only primary producer of the coculture system, Synechococcus, displayed metabolic processes primarily involved in inorganic nutrient uptake, photosynthesis, and organic matter biosynthesis and release.
32071270	7	36	from	genomes	1600:1606	arg1	present					1570:1576	present	1570:1576	present	1570:1576	Polysaccharide utilization loci present in the flavobacterial genomes influence their lifestyle preferences and close associations with phytoplankton.
32071270	5	37	theme	coculture	1070:1078	arg1	system					1080:1085	the coculture system	1066:1085	the coculture system	1066:1085	The only primary producer of the coculture system, Synechococcus, displayed metabolic processes primarily involved in inorganic nutrient uptake, photosynthesis, and organic matter biosynthesis and release.
32071270	14	38	theme	uncultured	2868:2877	arg1	populations					2898:2908	uncultured dominant bacterial populations	2868:2908	uncultured dominant bacterial populations	2868:2908	In the present study, combined metagenomic and metaproteomic data supplied the metabolic potentials and activities of uncultured dominant bacterial populations in the coculture system.
32071270	1	39	from	diversity	216:224	arg1	environments					255:266	upper ocean environments	243:266	upper ocean environments	243:266	Microbial photoautotroph-heterotroph interactions underlie marine food webs and shape ecosystem diversity and structure in upper ocean environments.
32071270	5	40	theme	only	1041:1044	arg1	producer					1054:1061	The only primary producer	1037:1061	The only primary producer of the coculture system	1037:1085	The only primary producer of the coculture system, Synechococcus, displayed metabolic processes primarily involved in inorganic nutrient uptake, photosynthesis, and organic matter biosynthesis and release.
32071270	5	40	theme	only	1041:1044	arg1	Synechococcus					1088:1100	Synechococcus	1088:1100	Synechococcus	1088:1100	The only primary producer of the coculture system, Synechococcus, displayed metabolic processes primarily involved in inorganic nutrient uptake, photosynthesis, and organic matter biosynthesis and release.
32071270	14	41	from	activities	2854:2863	arg1	system					2927:2932	the coculture system	2913:2932	the coculture system	2913:2932	In the present study, combined metagenomic and metaproteomic data supplied the metabolic potentials and activities of uncultured dominant bacterial populations in the coculture system.
32071270	15	42	theme	study	2955:2959	arg1	results					2939:2945	The results	2935:2945	The results of this study	2935:2959	The results of this study shed light on the nature of interactions between photoautotrophs and heterotrophs, improving our understanding of the complexity of in situ environments.
32071270	1	43	theme	ecosystem	206:214	arg1	diversity					216:224	ecosystem diversity	206:224	ecosystem diversity	206:224	Microbial photoautotroph-heterotroph interactions underlie marine food webs and shape ecosystem diversity and structure in upper ocean environments.
32071270	8	44	theme	Oricola	1727:1733	arg1	sp					1735:1736	the alphaproteobacterium Oricola sp	1702:1736	the alphaproteobacterium Oricola sp	1702:1736	In contrast, the alphaproteobacterium Oricola sp.
32071270	9	45	theme	dissolved	1787:1795	arg1	DOC					1813:1815	DOC	1813:1815	DOC	1813:1815	population mainly utilized low-molecular-weight dissolved organic carbon (DOC) through ATP-binding cassette (ABC), tripartite ATP-independent periplasmic (TRAP), and tripartite tricarboxylate transporter (TTT) transport systems.
32071270	9	45	theme	dissolved	1787:1795	arg1	carbon					1805:1810	dissolved organic carbon	1787:1810	dissolved organic carbon (DOC) through ATP-binding cassette (ABC)	1787:1851	population mainly utilized low-molecular-weight dissolved organic carbon (DOC) through ATP-binding cassette (ABC), tripartite ATP-independent periplasmic (TRAP), and tripartite tricarboxylate transporter (TTT) transport systems.
32071270	1	46	theme	food	186:189	arg1	webs					191:194	marine food webs	179:194	marine food webs	179:194	Microbial photoautotroph-heterotroph interactions underlie marine food webs and shape ecosystem diversity and structure in upper ocean environments.
32071270	3	47	theme	heterotrophic	525:537	arg1	assembly					549:556	The associated heterotrophic bacterial assembly	510:556	The associated heterotrophic bacterial assembly	510:556	The associated heterotrophic bacterial assembly mostly constituted five classes, including Flavobacteria, Bacteroidetes, Phycisphaerae, Gammaproteobacteria, and Alphaproteobacteria The seven most abundant taxa/genera comprised >90% of the total heterotrophic bacterial community, and five of these displayed distinct lifestyle preferences (free-living or attached) and responses to Synechococcus growth phases.
32071270	6	48	theme	peptidase	1518:1526	arg1	proteins					1528:1535	peptidase proteins	1518:1535	peptidase proteins	1518:1535	Two of the flavobacterial populations, Muricauda and Winogradskyella, and an SM1A02 population, displayed preferences for initial degradation of complex compounds and biopolymers, as evinced by high abundances of TonB-dependent transporters (TBDTs), glycoside hydrolase, and peptidase proteins.
32071270	3	49	dep	Flavobacteria	601:613	arg1	taxa/genera					715:725	The seven most abundant taxa/genera	691:725	The seven most abundant taxa/genera	691:725	The associated heterotrophic bacterial assembly mostly constituted five classes, including Flavobacteria, Bacteroidetes, Phycisphaerae, Gammaproteobacteria, and Alphaproteobacteria The seven most abundant taxa/genera comprised >90% of the total heterotrophic bacterial community, and five of these displayed distinct lifestyle preferences (free-living or attached) and responses to Synechococcus growth phases.
32071270	12	50	theme	strains	2574:2580	arg1	systems					2510:2516	Two-member coculture systems	2489:2516	Two-member coculture systems of picocyanobacteria and single heterotrophic bacterial strains	2489:2580	Two-member coculture systems of picocyanobacteria and single heterotrophic bacterial strains have been thoroughly investigated.
32071270	2	51	theme	associated	455:464	arg1	heterotrophs					466:477	its associated heterotrophs	451:477	its associated heterotrophs	451:477	Here, bacterial community composition, lifestyle preference, and genomic- and proteomic-level metabolic characteristics were investigated for an open ocean Synechococcus ecotype and its associated heterotrophs over 91 days of cocultivation.
32071270	0	52	theme	Metagenomic	0:10	arg1	Insights					30:37	Metagenomic and Metaproteomic Insights	0:37	Metagenomic and Metaproteomic Insights into Photoautotrophic and Heterotrophic Interactions in a Synechococcus Culture	0:117	Metagenomic and Metaproteomic Insights into Photoautotrophic and Heterotrophic Interactions in a Synechococcus Culture.
32071270	0	53	theme	Metaproteomic	16:28	arg1	Insights					30:37	Metagenomic and Metaproteomic Insights	0:37	Metagenomic and Metaproteomic Insights into Photoautotrophic and Heterotrophic Interactions in a Synechococcus Culture	0:117	Metagenomic and Metaproteomic Insights into Photoautotrophic and Heterotrophic Interactions in a Synechococcus Culture.
32071270	11	54	theme	system	2301:2306	arg1	maintenance					2246:2256	the maintenance	2242:2256	the maintenance of the Synechococcus-heterotroph coculture system	2242:2306	In addition to nutrient exchange, removal of reactive oxygen species and vitamin trafficking might also contribute to the maintenance of the Synechococcus-heterotroph coculture system and the interactions shaping the system.IMPORTANCE The high complexity of in situ ecosystems renders it difficult to study marine microbial photoautotroph-heterotroph interactions.
32071270	11	54	theme	system	2301:2306	arg1	interactions					2316:2327	the interactions shaping the system.IMPORTANCE The high complexity of in situ ecosystems renders it difficult to study marine microbial photoautotroph-heterotroph interactions	2312:2486	the interactions shaping the system.IMPORTANCE The high complexity of in situ ecosystems renders it difficult to study marine microbial photoautotroph-heterotroph interactions	2312:2486	In addition to nutrient exchange, removal of reactive oxygen species and vitamin trafficking might also contribute to the maintenance of the Synechococcus-heterotroph coculture system and the interactions shaping the system.IMPORTANCE The high complexity of in situ ecosystems renders it difficult to study marine microbial photoautotroph-heterotroph interactions.
32071270	1	55	theme	photoautotroph-heterotroph	130:155	arg1	interactions					157:168	Microbial photoautotroph-heterotroph interactions	120:168	Microbial photoautotroph-heterotroph interactions	120:168	Microbial photoautotroph-heterotroph interactions underlie marine food webs and shape ecosystem diversity and structure in upper ocean environments.
32071270	7	56	from	present	1570:1576	arg1	genomes					1600:1606	the flavobacterial genomes	1581:1606	the flavobacterial genomes	1581:1606	Polysaccharide utilization loci present in the flavobacterial genomes influence their lifestyle preferences and close associations with phytoplankton.
32071270	11	57	theme	vitamin	2197:2203	arg1	trafficking					2205:2215	vitamin trafficking	2197:2215	vitamin trafficking	2197:2215	In addition to nutrient exchange, removal of reactive oxygen species and vitamin trafficking might also contribute to the maintenance of the Synechococcus-heterotroph coculture system and the interactions shaping the system.IMPORTANCE The high complexity of in situ ecosystems renders it difficult to study marine microbial photoautotroph-heterotroph interactions.
32071270	12	58	theme	coculture	2500:2508	arg1	systems					2510:2516	Two-member coculture systems	2489:2516	Two-member coculture systems of picocyanobacteria and single heterotrophic bacterial strains	2489:2580	Two-member coculture systems of picocyanobacteria and single heterotrophic bacterial strains have been thoroughly investigated.
32071270	3	59	theme	bacterial	769:777	arg1	community					779:787	the total heterotrophic bacterial community	745:787	the total heterotrophic bacterial community	745:787	The associated heterotrophic bacterial assembly mostly constituted five classes, including Flavobacteria, Bacteroidetes, Phycisphaerae, Gammaproteobacteria, and Alphaproteobacteria The seven most abundant taxa/genera comprised >90% of the total heterotrophic bacterial community, and five of these displayed distinct lifestyle preferences (free-living or attached) and responses to Synechococcus growth phases.
32071270	12	60	theme	heterotrophic	2550:2562	arg1	strains					2574:2580	picocyanobacteria and single heterotrophic bacterial strains	2521:2580	picocyanobacteria and single heterotrophic bacterial strains	2521:2580	Two-member coculture systems of picocyanobacteria and single heterotrophic bacterial strains have been thoroughly investigated.
32071270	11	61	theme	photoautotroph-heterotroph	2448:2473	arg1	interactions					2475:2486	marine microbial photoautotroph-heterotroph interactions	2431:2486	marine microbial photoautotroph-heterotroph interactions	2431:2486	In addition to nutrient exchange, removal of reactive oxygen species and vitamin trafficking might also contribute to the maintenance of the Synechococcus-heterotroph coculture system and the interactions shaping the system.IMPORTANCE The high complexity of in situ ecosystems renders it difficult to study marine microbial photoautotroph-heterotroph interactions.
32071270	2	62	theme	open	414:417	arg1	ecotype					439:445	an open ocean Synechococcus ecotype	411:445	an open ocean Synechococcus ecotype	411:445	Here, bacterial community composition, lifestyle preference, and genomic- and proteomic-level metabolic characteristics were investigated for an open ocean Synechococcus ecotype and its associated heterotrophs over 91 days of cocultivation.
32071270	15	63	dep	in	3093:3094	arg1	situ					3096:3099	situ	3096:3099	situ	3096:3099	The results of this study shed light on the nature of interactions between photoautotrophs and heterotrophs, improving our understanding of the complexity of in situ environments.
32071270	3	64	theme	these	802:806	arg1	five					794:797	five	794:797	five	794:797	The associated heterotrophic bacterial assembly mostly constituted five classes, including Flavobacteria, Bacteroidetes, Phycisphaerae, Gammaproteobacteria, and Alphaproteobacteria The seven most abundant taxa/genera comprised >90% of the total heterotrophic bacterial community, and five of these displayed distinct lifestyle preferences (free-living or attached) and responses to Synechococcus growth phases.
32071270	3	64	theme	these	802:806	arg1	these					802:806	these	802:806	these	802:806	The associated heterotrophic bacterial assembly mostly constituted five classes, including Flavobacteria, Bacteroidetes, Phycisphaerae, Gammaproteobacteria, and Alphaproteobacteria The seven most abundant taxa/genera comprised >90% of the total heterotrophic bacterial community, and five of these displayed distinct lifestyle preferences (free-living or attached) and responses to Synechococcus growth phases.
32071270	4	65	theme	high-quality	925:936	arg1	genomes					938:944	Six high-quality genomes	921:944	Six high-quality genomes	921:944	Six high-quality genomes, including Synechococcus and the five dominant heterotrophic bacteria, were reconstructed.
32071270	4	65	theme	high-quality	925:936	arg1	Synechococcus					957:969	Synechococcus	957:969	Synechococcus	957:969	Six high-quality genomes, including Synechococcus and the five dominant heterotrophic bacteria, were reconstructed.
32071270	4	65	theme	high-quality	925:936	arg1	bacteria					1007:1014	the five dominant heterotrophic bacteria	975:1014	the five dominant heterotrophic bacteria	975:1014	Six high-quality genomes, including Synechococcus and the five dominant heterotrophic bacteria, were reconstructed.
32071270	14	66	theme	metabolic	2829:2837	arg1	potentials					2839:2848	potentials	2839:2848	potentials	2839:2848	In the present study, combined metagenomic and metaproteomic data supplied the metabolic potentials and activities of uncultured dominant bacterial populations in the coculture system.
32071270	7	67	with	associations	1656:1667	arg1	phytoplankton					1674:1686	phytoplankton	1674:1686	phytoplankton	1674:1686	Polysaccharide utilization loci present in the flavobacterial genomes influence their lifestyle preferences and close associations with phytoplankton.
32071270	5	68	theme	inorganic	1155:1163	arg1	uptake					1174:1179	inorganic nutrient uptake	1155:1179	inorganic nutrient uptake	1155:1179	The only primary producer of the coculture system, Synechococcus, displayed metabolic processes primarily involved in inorganic nutrient uptake, photosynthesis, and organic matter biosynthesis and release.
32071270	13	69	theme	in	2626:2627	arg1	interactions					2634:2645	in situ interactions	2626:2645	in situ interactions	2626:2645	However, in situ interactions comprise far more diverse heterotrophic bacterial associations with single photoautotrophic organisms.
32071270	15	70	theme	interactions	2989:3000	arg1	nature					2979:2984	the nature	2975:2984	the nature of interactions between photoautotrophs and heterotrophs	2975:3041	The results of this study shed light on the nature of interactions between photoautotrophs and heterotrophs, improving our understanding of the complexity of in situ environments.
32071270	7	71	with	preferences	1634:1644	arg1	phytoplankton					1674:1686	phytoplankton	1674:1686	phytoplankton	1674:1686	Polysaccharide utilization loci present in the flavobacterial genomes influence their lifestyle preferences and close associations with phytoplankton.
32071270	14	72	theme	coculture	2917:2925	arg1	system					2927:2932	the coculture system	2913:2932	the coculture system	2913:2932	In the present study, combined metagenomic and metaproteomic data supplied the metabolic potentials and activities of uncultured dominant bacterial populations in the coculture system.
32071270	6	73	theme	populations	1269:1279	arg1	populations					1269:1279	the flavobacterial populations	1250:1279	the flavobacterial populations	1250:1279	Two of the flavobacterial populations, Muricauda and Winogradskyella, and an SM1A02 population, displayed preferences for initial degradation of complex compounds and biopolymers, as evinced by high abundances of TonB-dependent transporters (TBDTs), glycoside hydrolase, and peptidase proteins.
32071270	6	73	theme	populations	1269:1279	arg1	Two					1243:1245	Two	1243:1245	Two	1243:1245	Two of the flavobacterial populations, Muricauda and Winogradskyella, and an SM1A02 population, displayed preferences for initial degradation of complex compounds and biopolymers, as evinced by high abundances of TonB-dependent transporters (TBDTs), glycoside hydrolase, and peptidase proteins.
32071270	15	74	theme	environments	3101:3112	arg1	complexity					3079:3088	the complexity	3075:3088	the complexity of in situ environments	3075:3112	The results of this study shed light on the nature of interactions between photoautotrophs and heterotrophs, improving our understanding of the complexity of in situ environments.
32071270	6	75	theme	transporters	1471:1482	arg1	abundances					1442:1451	high abundances	1437:1451	high abundances of TonB-dependent transporters (TBDTs), glycoside hydrolase, and peptidase proteins	1437:1535	Two of the flavobacterial populations, Muricauda and Winogradskyella, and an SM1A02 population, displayed preferences for initial degradation of complex compounds and biopolymers, as evinced by high abundances of TonB-dependent transporters (TBDTs), glycoside hydrolase, and peptidase proteins.
32071270	14	76	theme	metaproteomic	2797:2809	arg1	data					2811:2814	combined metagenomic and metaproteomic data	2772:2814	combined metagenomic and metaproteomic data	2772:2814	In the present study, combined metagenomic and metaproteomic data supplied the metabolic potentials and activities of uncultured dominant bacterial populations in the coculture system.
32071270	2	77	theme	Synechococcus	425:437	arg1	ecotype					439:445	an open ocean Synechococcus ecotype	411:445	an open ocean Synechococcus ecotype	411:445	Here, bacterial community composition, lifestyle preference, and genomic- and proteomic-level metabolic characteristics were investigated for an open ocean Synechococcus ecotype and its associated heterotrophs over 91 days of cocultivation.
32071270	8	78	dep	sp	1735:1736	arg1	contrast					1692:1699	contrast	1692:1699	contrast	1692:1699	In contrast, the alphaproteobacterium Oricola sp.
32071270	11	79	theme	marine	2431:2436	arg1	interactions					2475:2486	marine microbial photoautotroph-heterotroph interactions	2431:2486	marine microbial photoautotroph-heterotroph interactions	2431:2486	In addition to nutrient exchange, removal of reactive oxygen species and vitamin trafficking might also contribute to the maintenance of the Synechococcus-heterotroph coculture system and the interactions shaping the system.IMPORTANCE The high complexity of in situ ecosystems renders it difficult to study marine microbial photoautotroph-heterotroph interactions.
32071270	3	80	theme	distinct	818:825	arg1	preferences					837:847	distinct lifestyle preferences	818:847	distinct lifestyle preferences (free-living or attached)	818:873	The associated heterotrophic bacterial assembly mostly constituted five classes, including Flavobacteria, Bacteroidetes, Phycisphaerae, Gammaproteobacteria, and Alphaproteobacteria The seven most abundant taxa/genera comprised >90% of the total heterotrophic bacterial community, and five of these displayed distinct lifestyle preferences (free-living or attached) and responses to Synechococcus growth phases.
32071270	14	81	theme	metagenomic	2781:2791	arg1	data					2811:2814	combined metagenomic and metaproteomic data	2772:2814	combined metagenomic and metaproteomic data	2772:2814	In the present study, combined metagenomic and metaproteomic data supplied the metabolic potentials and activities of uncultured dominant bacterial populations in the coculture system.
32071270	14	82	theme	bacterial	2888:2896	arg1	populations					2898:2908	uncultured dominant bacterial populations	2868:2908	uncultured dominant bacterial populations	2868:2908	In the present study, combined metagenomic and metaproteomic data supplied the metabolic potentials and activities of uncultured dominant bacterial populations in the coculture system.
32071270	11	83	dep	system.IMPORTANCE	2341:2357	arg1	renders					2401:2407	renders	2401:2407	renders it difficult to study marine microbial photoautotroph-heterotroph interactions	2401:2486	In addition to nutrient exchange, removal of reactive oxygen species and vitamin trafficking might also contribute to the maintenance of the Synechococcus-heterotroph coculture system and the interactions shaping the system.IMPORTANCE The high complexity of in situ ecosystems renders it difficult to study marine microbial photoautotroph-heterotroph interactions.
32071270	6	84	theme	high	1437:1440	arg1	abundances					1442:1451	high abundances	1437:1451	high abundances of TonB-dependent transporters (TBDTs), glycoside hydrolase, and peptidase proteins	1437:1535	Two of the flavobacterial populations, Muricauda and Winogradskyella, and an SM1A02 population, displayed preferences for initial degradation of complex compounds and biopolymers, as evinced by high abundances of TonB-dependent transporters (TBDTs), glycoside hydrolase, and peptidase proteins.
32071270	10	85	theme	bacterial	1986:1994	arg1	populations					1996:2006	The heterotrophic bacterial populations	1968:2006	The heterotrophic bacterial populations	1968:2006	The heterotrophic bacterial populations exhibited complementary mechanisms for degrading Synechococcus-derived organic matter and driving nutrient cycling.
32071270	13	86	theme	single	2715:2720	arg1	organisms					2739:2747	single photoautotrophic organisms	2715:2747	single photoautotrophic organisms	2715:2747	However, in situ interactions comprise far more diverse heterotrophic bacterial associations with single photoautotrophic organisms.
32071270	7	87	theme	close	1650:1654	arg1	associations					1656:1667	close associations	1650:1667	close associations with phytoplankton	1650:1686	Polysaccharide utilization loci present in the flavobacterial genomes influence their lifestyle preferences and close associations with phytoplankton.
32071270	7	88	theme	lifestyle	1624:1632	arg1	preferences					1634:1644	their lifestyle preferences	1618:1644	their lifestyle preferences	1618:1644	Polysaccharide utilization loci present in the flavobacterial genomes influence their lifestyle preferences and close associations with phytoplankton.
32071270	14	89	theme	present	2757:2763	arg1	study					2765:2769	the present study	2753:2769	the present study	2753:2769	In the present study, combined metagenomic and metaproteomic data supplied the metabolic potentials and activities of uncultured dominant bacterial populations in the coculture system.
32071270	4	90	theme	heterotrophic	993:1005	arg1	bacteria					1007:1014	the five dominant heterotrophic bacteria	975:1014	the five dominant heterotrophic bacteria	975:1014	Six high-quality genomes, including Synechococcus and the five dominant heterotrophic bacteria, were reconstructed.
32071270	6	91	theme	SM1A02	1320:1325	arg1	population					1327:1336	an SM1A02 population	1317:1336	an SM1A02 population	1317:1336	Two of the flavobacterial populations, Muricauda and Winogradskyella, and an SM1A02 population, displayed preferences for initial degradation of complex compounds and biopolymers, as evinced by high abundances of TonB-dependent transporters (TBDTs), glycoside hydrolase, and peptidase proteins.
32071270	2	92	theme	metabolic	363:371	arg1	characteristics					373:387	genomic- and proteomic-level metabolic characteristics	334:387	genomic- and proteomic-level metabolic characteristics	334:387	Here, bacterial community composition, lifestyle preference, and genomic- and proteomic-level metabolic characteristics were investigated for an open ocean Synechococcus ecotype and its associated heterotrophs over 91 days of cocultivation.
32071270	1	93	theme	upper	243:247	arg1	environments					255:266	upper ocean environments	243:266	upper ocean environments	243:266	Microbial photoautotroph-heterotroph interactions underlie marine food webs and shape ecosystem diversity and structure in upper ocean environments.
32071270	11	94	theme	in	2382:2383	arg1	ecosystems					2390:2399	in situ ecosystems	2382:2399	in situ ecosystems	2382:2399	In addition to nutrient exchange, removal of reactive oxygen species and vitamin trafficking might also contribute to the maintenance of the Synechococcus-heterotroph coculture system and the interactions shaping the system.IMPORTANCE The high complexity of in situ ecosystems renders it difficult to study marine microbial photoautotroph-heterotroph interactions.
32071270	7	95	theme	flavobacterial	1585:1598	arg1	genomes					1600:1606	the flavobacterial genomes	1581:1606	the flavobacterial genomes	1581:1606	Polysaccharide utilization loci present in the flavobacterial genomes influence their lifestyle preferences and close associations with phytoplankton.
32071270	6	96	theme	hydrolase	1503:1511	arg1	abundances					1442:1451	high abundances	1437:1451	high abundances of TonB-dependent transporters (TBDTs), glycoside hydrolase, and peptidase proteins	1437:1535	Two of the flavobacterial populations, Muricauda and Winogradskyella, and an SM1A02 population, displayed preferences for initial degradation of complex compounds and biopolymers, as evinced by high abundances of TonB-dependent transporters (TBDTs), glycoside hydrolase, and peptidase proteins.
32071270	9	97	theme	tricarboxylate	1916:1929	arg1	TTT					1944:1946	TTT	1944:1946	TTT	1944:1946	population mainly utilized low-molecular-weight dissolved organic carbon (DOC) through ATP-binding cassette (ABC), tripartite ATP-independent periplasmic (TRAP), and tripartite tricarboxylate transporter (TTT) transport systems.
32071270	9	97	theme	tricarboxylate	1916:1929	arg1	transporter					1931:1941	tripartite tricarboxylate transporter	1905:1941	tripartite tricarboxylate transporter (TTT)	1905:1947	population mainly utilized low-molecular-weight dissolved organic carbon (DOC) through ATP-binding cassette (ABC), tripartite ATP-independent periplasmic (TRAP), and tripartite tricarboxylate transporter (TTT) transport systems.
32071270	2	98	theme	community	285:293	arg1	composition					295:305	bacterial community composition	275:305	bacterial community composition	275:305	Here, bacterial community composition, lifestyle preference, and genomic- and proteomic-level metabolic characteristics were investigated for an open ocean Synechococcus ecotype and its associated heterotrophs over 91 days of cocultivation.
32071270	6	99	theme	biopolymers	1410:1420	arg1	degradation					1373:1383	initial degradation	1365:1383	initial degradation of complex compounds and biopolymers	1365:1420	Two of the flavobacterial populations, Muricauda and Winogradskyella, and an SM1A02 population, displayed preferences for initial degradation of complex compounds and biopolymers, as evinced by high abundances of TonB-dependent transporters (TBDTs), glycoside hydrolase, and peptidase proteins.
32071270	3	100	theme	abundant	706:713	arg1	taxa/genera					715:725	The seven most abundant taxa/genera	691:725	The seven most abundant taxa/genera	691:725	The associated heterotrophic bacterial assembly mostly constituted five classes, including Flavobacteria, Bacteroidetes, Phycisphaerae, Gammaproteobacteria, and Alphaproteobacteria The seven most abundant taxa/genera comprised >90% of the total heterotrophic bacterial community, and five of these displayed distinct lifestyle preferences (free-living or attached) and responses to Synechococcus growth phases.
32071270	11	101	dep	interactions	2316:2327	arg1	shaping					2329:2335	shaping the system.IMPORTANCE The high complexity of in situ ecosystems renders it difficult to study marine microbial photoautotroph-heterotroph interactions	2329:2486	the interactions shaping the system.IMPORTANCE The high complexity of in situ ecosystems renders it difficult to study marine microbial photoautotroph-heterotroph interactions	2312:2486	In addition to nutrient exchange, removal of reactive oxygen species and vitamin trafficking might also contribute to the maintenance of the Synechococcus-heterotroph coculture system and the interactions shaping the system.IMPORTANCE The high complexity of in situ ecosystems renders it difficult to study marine microbial photoautotroph-heterotroph interactions.
32071270	6	102	theme	compounds	1396:1404	arg1	degradation					1373:1383	initial degradation	1365:1383	initial degradation of complex compounds and biopolymers	1365:1420	Two of the flavobacterial populations, Muricauda and Winogradskyella, and an SM1A02 population, displayed preferences for initial degradation of complex compounds and biopolymers, as evinced by high abundances of TonB-dependent transporters (TBDTs), glycoside hydrolase, and peptidase proteins.
32071270	3	103	theme	growth	906:911	arg1	phases					913:918	Synechococcus growth phases	892:918	Synechococcus growth phases	892:918	The associated heterotrophic bacterial assembly mostly constituted five classes, including Flavobacteria, Bacteroidetes, Phycisphaerae, Gammaproteobacteria, and Alphaproteobacteria The seven most abundant taxa/genera comprised >90% of the total heterotrophic bacterial community, and five of these displayed distinct lifestyle preferences (free-living or attached) and responses to Synechococcus growth phases.
32071270	5	104	theme	matter	1210:1215	arg1	biosynthesis					1217:1228	organic matter biosynthesis	1202:1228	organic matter biosynthesis	1202:1228	The only primary producer of the coculture system, Synechococcus, displayed metabolic processes primarily involved in inorganic nutrient uptake, photosynthesis, and organic matter biosynthesis and release.
32071270	11	105	theme	Synechococcus-heterotroph	2265:2289	arg1	system					2301:2306	the Synechococcus-heterotroph coculture system	2261:2306	the Synechococcus-heterotroph coculture system	2261:2306	In addition to nutrient exchange, removal of reactive oxygen species and vitamin trafficking might also contribute to the maintenance of the Synechococcus-heterotroph coculture system and the interactions shaping the system.IMPORTANCE The high complexity of in situ ecosystems renders it difficult to study marine microbial photoautotroph-heterotroph interactions.
32071270	1	106	theme	marine	179:184	arg1	webs					191:194	marine food webs	179:194	marine food webs	179:194	Microbial photoautotroph-heterotroph interactions underlie marine food webs and shape ecosystem diversity and structure in upper ocean environments.
32071270	15	107	theme	complexity	3079:3088	arg1	understanding					3058:3070	our understanding	3054:3070	our understanding of the complexity of in situ environments	3054:3112	The results of this study shed light on the nature of interactions between photoautotrophs and heterotrophs, improving our understanding of the complexity of in situ environments.
32071270	11	108	theme	ecosystems	2390:2399	arg1	complexity					2368:2377	The high complexity	2359:2377	The high complexity of in situ ecosystems	2359:2399	In addition to nutrient exchange, removal of reactive oxygen species and vitamin trafficking might also contribute to the maintenance of the Synechococcus-heterotroph coculture system and the interactions shaping the system.IMPORTANCE The high complexity of in situ ecosystems renders it difficult to study marine microbial photoautotroph-heterotroph interactions.
32071270	7	109	theme	Polysaccharide	1538:1551	arg1	loci					1565:1568	Polysaccharide utilization loci	1538:1568	Polysaccharide utilization loci present in the flavobacterial genomes	1538:1606	Polysaccharide utilization loci present in the flavobacterial genomes influence their lifestyle preferences and close associations with phytoplankton.
32071270	14	110	dep	potentials	2839:2848	arg1	the					2825:2827	the	2825:2827	the	2825:2827	In the present study, combined metagenomic and metaproteomic data supplied the metabolic potentials and activities of uncultured dominant bacterial populations in the coculture system.
32071270	10	111	link	Synechococcus-derived	2057:2077	arg1	matter					2087:2092	Synechococcus-derived organic matter	2057:2092	Synechococcus-derived organic matter	2057:2092	The heterotrophic bacterial populations exhibited complementary mechanisms for degrading Synechococcus-derived organic matter and driving nutrient cycling.
32071270	10	112	theme	nutrient	2106:2113	arg1	cycling					2115:2121	nutrient cycling	2106:2121	nutrient cycling	2106:2121	The heterotrophic bacterial populations exhibited complementary mechanisms for degrading Synechococcus-derived organic matter and driving nutrient cycling.
32071270	11	113	dep	shaping	2329:2335	arg1	system.IMPORTANCE					2341:2357	the system.IMPORTANCE	2337:2357	the interactions shaping the system.IMPORTANCE The high complexity of in situ ecosystems renders it difficult to study marine microbial photoautotroph-heterotroph interactions	2312:2486	In addition to nutrient exchange, removal of reactive oxygen species and vitamin trafficking might also contribute to the maintenance of the Synechococcus-heterotroph coculture system and the interactions shaping the system.IMPORTANCE The high complexity of in situ ecosystems renders it difficult to study marine microbial photoautotroph-heterotroph interactions.
32071270	8	114	theme	alphaproteobacterium	1706:1725	arg1	sp					1735:1736	the alphaproteobacterium Oricola sp	1702:1736	the alphaproteobacterium Oricola sp	1702:1736	In contrast, the alphaproteobacterium Oricola sp.
32071270	6	115	theme	initial	1365:1371	arg1	degradation					1373:1383	initial degradation	1365:1383	initial degradation of complex compounds and biopolymers	1365:1420	Two of the flavobacterial populations, Muricauda and Winogradskyella, and an SM1A02 population, displayed preferences for initial degradation of complex compounds and biopolymers, as evinced by high abundances of TonB-dependent transporters (TBDTs), glycoside hydrolase, and peptidase proteins.
32071270	2	116	theme	lifestyle	308:316	arg1	preference					318:327	lifestyle preference	308:327	lifestyle preference	308:327	Here, bacterial community composition, lifestyle preference, and genomic- and proteomic-level metabolic characteristics were investigated for an open ocean Synechococcus ecotype and its associated heterotrophs over 91 days of cocultivation.
32071270	0	117	theme	Synechococcus	97:109	arg1	Culture					111:117	a Synechococcus Culture	95:117	a Synechococcus Culture	95:117	Metagenomic and Metaproteomic Insights into Photoautotrophic and Heterotrophic Interactions in a Synechococcus Culture.
32071270	5	118	theme	primary	1046:1052	arg1	producer					1054:1061	The only primary producer	1037:1061	The only primary producer of the coculture system	1037:1085	The only primary producer of the coculture system, Synechococcus, displayed metabolic processes primarily involved in inorganic nutrient uptake, photosynthesis, and organic matter biosynthesis and release.
32071270	5	118	theme	primary	1046:1052	arg1	Synechococcus					1088:1100	Synechococcus	1088:1100	Synechococcus	1088:1100	The only primary producer of the coculture system, Synechococcus, displayed metabolic processes primarily involved in inorganic nutrient uptake, photosynthesis, and organic matter biosynthesis and release.
32071270	7	119	attach	present	1570:1576	arg1	genomes					1600:1606	the flavobacterial genomes	1581:1606	the flavobacterial genomes	1581:1606	Polysaccharide utilization loci present in the flavobacterial genomes influence their lifestyle preferences and close associations with phytoplankton.
32071270	7	119	attach	present	1570:1576	arg2	loci					1565:1568	Polysaccharide utilization loci	1538:1568	Polysaccharide utilization loci present in the flavobacterial genomes	1538:1606	Polysaccharide utilization loci present in the flavobacterial genomes influence their lifestyle preferences and close associations with phytoplankton.
32071270	15	120	theme	in	3093:3094	arg1	environments					3101:3112	in situ environments	3093:3112	in situ environments	3093:3112	The results of this study shed light on the nature of interactions between photoautotrophs and heterotrophs, improving our understanding of the complexity of in situ environments.
32071270	5	121	theme	metabolic	1113:1121	arg1	processes					1123:1131	metabolic processes	1113:1131	metabolic processes primarily involved in inorganic nutrient uptake, photosynthesis, and organic matter biosynthesis and release	1113:1240	The only primary producer of the coculture system, Synechococcus, displayed metabolic processes primarily involved in inorganic nutrient uptake, photosynthesis, and organic matter biosynthesis and release.
32071270	10	122	theme	complementary	2018:2030	arg1	mechanisms					2032:2041	complementary mechanisms	2018:2041	complementary mechanisms for degrading Synechococcus-derived organic matter and driving nutrient cycling	2018:2121	The heterotrophic bacterial populations exhibited complementary mechanisms for degrading Synechococcus-derived organic matter and driving nutrient cycling.
32071270	13	123	dep	diverse	2665:2671	arg1	heterotrophic					2673:2685	heterotrophic	2673:2685	heterotrophic	2673:2685	However, in situ interactions comprise far more diverse heterotrophic bacterial associations with single photoautotrophic organisms.
32071270	6	124	theme	proteins	1528:1535	arg1	abundances					1442:1451	high abundances	1437:1451	high abundances of TonB-dependent transporters (TBDTs), glycoside hydrolase, and peptidase proteins	1437:1535	Two of the flavobacterial populations, Muricauda and Winogradskyella, and an SM1A02 population, displayed preferences for initial degradation of complex compounds and biopolymers, as evinced by high abundances of TonB-dependent transporters (TBDTs), glycoside hydrolase, and peptidase proteins.
32071270	13	125	theme	bacterial	2687:2695	arg1	associations					2697:2708	far more diverse heterotrophic bacterial associations	2656:2708	far more diverse heterotrophic bacterial associations with single photoautotrophic organisms	2656:2747	However, in situ interactions comprise far more diverse heterotrophic bacterial associations with single photoautotrophic organisms.
32071270	9	126	theme	organic	1797:1803	arg1	DOC					1813:1815	DOC	1813:1815	DOC	1813:1815	population mainly utilized low-molecular-weight dissolved organic carbon (DOC) through ATP-binding cassette (ABC), tripartite ATP-independent periplasmic (TRAP), and tripartite tricarboxylate transporter (TTT) transport systems.
32071270	9	126	theme	organic	1797:1803	arg1	carbon					1805:1810	dissolved organic carbon	1787:1810	dissolved organic carbon (DOC) through ATP-binding cassette (ABC)	1787:1851	population mainly utilized low-molecular-weight dissolved organic carbon (DOC) through ATP-binding cassette (ABC), tripartite ATP-independent periplasmic (TRAP), and tripartite tricarboxylate transporter (TTT) transport systems.
32071270	3	127	theme	bacterial	539:547	arg1	assembly					549:556	The associated heterotrophic bacterial assembly	510:556	The associated heterotrophic bacterial assembly	510:556	The associated heterotrophic bacterial assembly mostly constituted five classes, including Flavobacteria, Bacteroidetes, Phycisphaerae, Gammaproteobacteria, and Alphaproteobacteria The seven most abundant taxa/genera comprised >90% of the total heterotrophic bacterial community, and five of these displayed distinct lifestyle preferences (free-living or attached) and responses to Synechococcus growth phases.
32071270	11	128	dep	exchange	2148:2155	arg1	addition					2127:2134	addition	2127:2134	addition	2127:2134	In addition to nutrient exchange, removal of reactive oxygen species and vitamin trafficking might also contribute to the maintenance of the Synechococcus-heterotroph coculture system and the interactions shaping the system.IMPORTANCE The high complexity of in situ ecosystems renders it difficult to study marine microbial photoautotroph-heterotroph interactions.
32071270	12	129	theme	bacterial	2564:2572	arg1	strains					2574:2580	picocyanobacteria and single heterotrophic bacterial strains	2521:2580	picocyanobacteria and single heterotrophic bacterial strains	2521:2580	Two-member coculture systems of picocyanobacteria and single heterotrophic bacterial strains have been thoroughly investigated.
32071270	3	130	theme	associated	514:523	arg1	assembly					549:556	The associated heterotrophic bacterial assembly	510:556	The associated heterotrophic bacterial assembly	510:556	The associated heterotrophic bacterial assembly mostly constituted five classes, including Flavobacteria, Bacteroidetes, Phycisphaerae, Gammaproteobacteria, and Alphaproteobacteria The seven most abundant taxa/genera comprised >90% of the total heterotrophic bacterial community, and five of these displayed distinct lifestyle preferences (free-living or attached) and responses to Synechococcus growth phases.
32071270	3	131	dep	constituted	565:575	arg1	comprised					727:735	comprised	727:735	constituted five classes, including Flavobacteria, Bacteroidetes, Phycisphaerae, Gammaproteobacteria, and Alphaproteobacteria The seven most abundant taxa/genera comprised >90% of the total heterotrophic bacterial community	565:787	The associated heterotrophic bacterial assembly mostly constituted five classes, including Flavobacteria, Bacteroidetes, Phycisphaerae, Gammaproteobacteria, and Alphaproteobacteria The seven most abundant taxa/genera comprised >90% of the total heterotrophic bacterial community, and five of these displayed distinct lifestyle preferences (free-living or attached) and responses to Synechococcus growth phases.
32071270	13	132	with	associations	2697:2708	arg1	organisms					2739:2747	single photoautotrophic organisms	2715:2747	single photoautotrophic organisms	2715:2747	However, in situ interactions comprise far more diverse heterotrophic bacterial associations with single photoautotrophic organisms.
32071270	1	133	theme	Microbial	120:128	arg1	interactions					157:168	Microbial photoautotroph-heterotroph interactions	120:168	Microbial photoautotroph-heterotroph interactions	120:168	Microbial photoautotroph-heterotroph interactions underlie marine food webs and shape ecosystem diversity and structure in upper ocean environments.
32071270	14	134	from	potentials	2839:2848	arg1	system					2927:2932	the coculture system	2913:2932	the coculture system	2913:2932	In the present study, combined metagenomic and metaproteomic data supplied the metabolic potentials and activities of uncultured dominant bacterial populations in the coculture system.
32071270	3	135	theme	community	779:787	arg1	community					779:787	the total heterotrophic bacterial community	745:787	the total heterotrophic bacterial community	745:787	The associated heterotrophic bacterial assembly mostly constituted five classes, including Flavobacteria, Bacteroidetes, Phycisphaerae, Gammaproteobacteria, and Alphaproteobacteria The seven most abundant taxa/genera comprised >90% of the total heterotrophic bacterial community, and five of these displayed distinct lifestyle preferences (free-living or attached) and responses to Synechococcus growth phases.
32071270	3	135	theme	community	779:787	arg1	%					740:740	>90%	737:740	>90% of the total heterotrophic bacterial community	737:787	The associated heterotrophic bacterial assembly mostly constituted five classes, including Flavobacteria, Bacteroidetes, Phycisphaerae, Gammaproteobacteria, and Alphaproteobacteria The seven most abundant taxa/genera comprised >90% of the total heterotrophic bacterial community, and five of these displayed distinct lifestyle preferences (free-living or attached) and responses to Synechococcus growth phases.
32071270	13	136	dep	in	2626:2627	arg1	situ					2629:2632	situ	2629:2632	situ	2629:2632	However, in situ interactions comprise far more diverse heterotrophic bacterial associations with single photoautotrophic organisms.
32071270	2	137	theme	cocultivation	495:507	arg1	91 days					484:490	91 days	484:490	91 days of cocultivation	484:507	Here, bacterial community composition, lifestyle preference, and genomic- and proteomic-level metabolic characteristics were investigated for an open ocean Synechococcus ecotype and its associated heterotrophs over 91 days of cocultivation.
32071270	0	138	theme	Photoautotrophic	44:59	arg1	Interactions					79:90	Photoautotrophic and Heterotrophic Interactions	44:90	Photoautotrophic and Heterotrophic Interactions in a Synechococcus Culture	44:117	Metagenomic and Metaproteomic Insights into Photoautotrophic and Heterotrophic Interactions in a Synechococcus Culture.
32071270	3	139	dep	preferences	837:847	arg1	free-living					850:860	free-living	850:860	free-living	850:860	The associated heterotrophic bacterial assembly mostly constituted five classes, including Flavobacteria, Bacteroidetes, Phycisphaerae, Gammaproteobacteria, and Alphaproteobacteria The seven most abundant taxa/genera comprised >90% of the total heterotrophic bacterial community, and five of these displayed distinct lifestyle preferences (free-living or attached) and responses to Synechococcus growth phases.
32071270	3	139	dep	preferences	837:847	arg1	attached					865:872	attached	865:872	attached	865:872	The associated heterotrophic bacterial assembly mostly constituted five classes, including Flavobacteria, Bacteroidetes, Phycisphaerae, Gammaproteobacteria, and Alphaproteobacteria The seven most abundant taxa/genera comprised >90% of the total heterotrophic bacterial community, and five of these displayed distinct lifestyle preferences (free-living or attached) and responses to Synechococcus growth phases.
32071270	9	140	theme	ATP-binding	1826:1836	arg1	ABC					1848:1850	ABC	1848:1850	ABC	1848:1850	population mainly utilized low-molecular-weight dissolved organic carbon (DOC) through ATP-binding cassette (ABC), tripartite ATP-independent periplasmic (TRAP), and tripartite tricarboxylate transporter (TTT) transport systems.
32071270	9	140	theme	ATP-binding	1826:1836	arg1	cassette					1838:1845	ATP-binding cassette	1826:1845	ATP-binding cassette (ABC)	1826:1851	population mainly utilized low-molecular-weight dissolved organic carbon (DOC) through ATP-binding cassette (ABC), tripartite ATP-independent periplasmic (TRAP), and tripartite tricarboxylate transporter (TTT) transport systems.
32071270	13	141	theme	diverse	2665:2671	arg1	associations					2697:2708	far more diverse heterotrophic bacterial associations	2656:2708	far more diverse heterotrophic bacterial associations with single photoautotrophic organisms	2656:2747	However, in situ interactions comprise far more diverse heterotrophic bacterial associations with single photoautotrophic organisms.
32071270	11	142	theme	species	2185:2191	arg1	removal					2158:2164	removal	2158:2164	removal of reactive oxygen species and vitamin trafficking	2158:2215	In addition to nutrient exchange, removal of reactive oxygen species and vitamin trafficking might also contribute to the maintenance of the Synechococcus-heterotroph coculture system and the interactions shaping the system.IMPORTANCE The high complexity of in situ ecosystems renders it difficult to study marine microbial photoautotroph-heterotroph interactions.
32071270	3	143	theme	heterotrophic	755:767	arg1	community					779:787	the total heterotrophic bacterial community	745:787	the total heterotrophic bacterial community	745:787	The associated heterotrophic bacterial assembly mostly constituted five classes, including Flavobacteria, Bacteroidetes, Phycisphaerae, Gammaproteobacteria, and Alphaproteobacteria The seven most abundant taxa/genera comprised >90% of the total heterotrophic bacterial community, and five of these displayed distinct lifestyle preferences (free-living or attached) and responses to Synechococcus growth phases.
32071270	12	144	theme	picocyanobacteria	2521:2537	arg1	strains					2574:2580	picocyanobacteria and single heterotrophic bacterial strains	2521:2580	picocyanobacteria and single heterotrophic bacterial strains	2521:2580	Two-member coculture systems of picocyanobacteria and single heterotrophic bacterial strains have been thoroughly investigated.
32071270	11	145	theme	trafficking	2205:2215	arg1	removal					2158:2164	removal	2158:2164	removal of reactive oxygen species and vitamin trafficking	2158:2215	In addition to nutrient exchange, removal of reactive oxygen species and vitamin trafficking might also contribute to the maintenance of the Synechococcus-heterotroph coculture system and the interactions shaping the system.IMPORTANCE The high complexity of in situ ecosystems renders it difficult to study marine microbial photoautotroph-heterotroph interactions.
32071270	0	146	theme	Heterotrophic	65:77	arg1	Interactions					79:90	Photoautotrophic and Heterotrophic Interactions	44:90	Photoautotrophic and Heterotrophic Interactions in a Synechococcus Culture	44:117	Metagenomic and Metaproteomic Insights into Photoautotrophic and Heterotrophic Interactions in a Synechococcus Culture.
32071270	9	147	theme	low-molecular-weight	1766:1785	arg1	systems					1959:1965	low-molecular-weight dissolved organic carbon (DOC) through ATP-binding cassette (ABC), tripartite ATP-independent periplasmic (TRAP), and tripartite tricarboxylate transporter (TTT) transport systems	1766:1965	low-molecular-weight dissolved organic carbon (DOC) through ATP-binding cassette (ABC), tripartite ATP-independent periplasmic (TRAP), and tripartite tricarboxylate transporter (TTT) transport systems	1766:1965	population mainly utilized low-molecular-weight dissolved organic carbon (DOC) through ATP-binding cassette (ABC), tripartite ATP-independent periplasmic (TRAP), and tripartite tricarboxylate transporter (TTT) transport systems.
32071270	12	148	theme	single	2543:2548	arg1	strains					2574:2580	picocyanobacteria and single heterotrophic bacterial strains	2521:2580	picocyanobacteria and single heterotrophic bacterial strains	2521:2580	Two-member coculture systems of picocyanobacteria and single heterotrophic bacterial strains have been thoroughly investigated.
32071270	11	149	theme	nutrient	2139:2146	arg1	exchange					2148:2155	nutrient exchange	2139:2155	nutrient exchange	2139:2155	In addition to nutrient exchange, removal of reactive oxygen species and vitamin trafficking might also contribute to the maintenance of the Synechococcus-heterotroph coculture system and the interactions shaping the system.IMPORTANCE The high complexity of in situ ecosystems renders it difficult to study marine microbial photoautotroph-heterotroph interactions.
32071270	9	150	theme	carbon	1805:1810	arg1	systems					1959:1965	low-molecular-weight dissolved organic carbon (DOC) through ATP-binding cassette (ABC), tripartite ATP-independent periplasmic (TRAP), and tripartite tricarboxylate transporter (TTT) transport systems	1766:1965	low-molecular-weight dissolved organic carbon (DOC) through ATP-binding cassette (ABC), tripartite ATP-independent periplasmic (TRAP), and tripartite tricarboxylate transporter (TTT) transport systems	1766:1965	population mainly utilized low-molecular-weight dissolved organic carbon (DOC) through ATP-binding cassette (ABC), tripartite ATP-independent periplasmic (TRAP), and tripartite tricarboxylate transporter (TTT) transport systems.
32071270	12	151	theme	Two-member	2489:2498	arg1	systems					2510:2516	Two-member coculture systems	2489:2516	Two-member coculture systems of picocyanobacteria and single heterotrophic bacterial strains	2489:2580	Two-member coculture systems of picocyanobacteria and single heterotrophic bacterial strains have been thoroughly investigated.
32071270	11	152	theme	coculture	2291:2299	arg1	system					2301:2306	the Synechococcus-heterotroph coculture system	2261:2306	the Synechococcus-heterotroph coculture system	2261:2306	In addition to nutrient exchange, removal of reactive oxygen species and vitamin trafficking might also contribute to the maintenance of the Synechococcus-heterotroph coculture system and the interactions shaping the system.IMPORTANCE The high complexity of in situ ecosystems renders it difficult to study marine microbial photoautotroph-heterotroph interactions.
32071270	5	153	theme	nutrient	1165:1172	arg1	uptake					1174:1179	inorganic nutrient uptake	1155:1179	inorganic nutrient uptake	1155:1179	The only primary producer of the coculture system, Synechococcus, displayed metabolic processes primarily involved in inorganic nutrient uptake, photosynthesis, and organic matter biosynthesis and release.
32071270	3	154	theme	lifestyle	827:835	arg1	preferences					837:847	distinct lifestyle preferences	818:847	distinct lifestyle preferences (free-living or attached)	818:873	The associated heterotrophic bacterial assembly mostly constituted five classes, including Flavobacteria, Bacteroidetes, Phycisphaerae, Gammaproteobacteria, and Alphaproteobacteria The seven most abundant taxa/genera comprised >90% of the total heterotrophic bacterial community, and five of these displayed distinct lifestyle preferences (free-living or attached) and responses to Synechococcus growth phases.
33891704	0	0	theme	hemocyanins	70:80	arg1	recognition					55:65	recognition	55:65	recognition of hemocyanins	55:80	C-type lectin receptors MR and DC-SIGN are involved in recognition of hemocyanins, shaping their immunostimulatory effects on human dendritic cells.
33891704	8	1	theme	clathrin-mediated	1211:1227	arg1	endocytosis					1229:1239	clathrin-mediated endocytosis	1211:1239	clathrin-mediated endocytosis	1211:1239	Moreover, hemocyanins colocalized with MR and DC-SIGN, and were partly internalized through clathrin-mediated endocytosis.
33891704	9	2	theme	hemocyanin-mediated	1246:1264	arg1	response					1291:1298	The hemocyanin-mediated proinflammatory cytokine response	1242:1298	The hemocyanin-mediated proinflammatory cytokine response	1242:1298	The hemocyanin-mediated proinflammatory cytokine response was impaired when using deglycosylated FLH and KLH compared to CCH.
33891704	9	2	theme	hemocyanin-mediated	1246:1264	arg1	impaired					1304:1311	impaired	1304:1311	impaired	1304:1311	The hemocyanin-mediated proinflammatory cytokine response was impaired when using deglycosylated FLH and KLH compared to CCH.
33891704	10	3	theme	human	1411:1415	arg1	MR					1417:1418	human MR	1411:1418	human MR	1411:1418	We further showed that hemocyanins bind to human MR and DC-SIGN in a carbohydrate-dependent manner with affinity constants in the physiological concentration range.
33891704	11	4	theme	valuable	1580:1587	arg1	hemocyanins					1589:1599	these three clinically valuable hemocyanins	1557:1599	these three clinically valuable hemocyanins	1557:1599	Overall, we showed that these three clinically valuable hemocyanins interact with human mannose-sensitive CLRs, initiating an immune response and promoting a Th1 cell-driving potential.
33891704	4	5	from	concholepas	586:596	arg1	glycans					561:567	hemocyanin glycans	550:567	hemocyanin glycans	550:567	We have previously shown that hemocyanin glycans from Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), participate in their immune recognition and immunogenicity in mice, interacting with murine C-type lectin receptors (CLRs).
33891704	9	6	theme	proinflammatory	1266:1280	arg1	response					1291:1298	The hemocyanin-mediated proinflammatory cytokine response	1242:1298	The hemocyanin-mediated proinflammatory cytokine response	1242:1298	The hemocyanin-mediated proinflammatory cytokine response was impaired when using deglycosylated FLH and KLH compared to CCH.
33891704	9	6	theme	proinflammatory	1266:1280	arg1	impaired					1304:1311	impaired	1304:1311	impaired	1304:1311	The hemocyanin-mediated proinflammatory cytokine response was impaired when using deglycosylated FLH and KLH compared to CCH.
33891704	2	7	theme	mannose-rich	379:390	arg1	structures					392:401	abundant and complex mannose-rich structures	358:401	abundant and complex mannose-rich structures	358:401	They are multiligand glycosylated molecules with abundant and complex mannose-rich structures.
33891704	11	8	theme	mannose-sensitive	1621:1637	arg1	CLRs					1639:1642	human mannose-sensitive CLRs	1615:1642	human mannose-sensitive CLRs	1615:1642	Overall, we showed that these three clinically valuable hemocyanins interact with human mannose-sensitive CLRs, initiating an immune response and promoting a Th1 cell-driving potential.
33891704	10	9	from	constants	1481:1489	arg1	range					1526:1530	the physiological concentration range	1494:1530	the physiological concentration range	1494:1530	We further showed that hemocyanins bind to human MR and DC-SIGN in a carbohydrate-dependent manner with affinity constants in the physiological concentration range.
33891704	4	10	from	crenulata	651:659	arg1	glycans					561:567	hemocyanin glycans	550:567	hemocyanin glycans	550:567	We have previously shown that hemocyanin glycans from Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), participate in their immune recognition and immunogenicity in mice, interacting with murine C-type lectin receptors (CLRs).
33891704	9	11	theme	cytokine	1282:1289	arg1	response					1291:1298	The hemocyanin-mediated proinflammatory cytokine response	1242:1298	The hemocyanin-mediated proinflammatory cytokine response	1242:1298	The hemocyanin-mediated proinflammatory cytokine response was impaired when using deglycosylated FLH and KLH compared to CCH.
33891704	9	11	theme	cytokine	1282:1289	arg1	impaired					1304:1311	impaired	1304:1311	impaired	1304:1311	The hemocyanin-mediated proinflammatory cytokine response was impaired when using deglycosylated FLH and KLH compared to CCH.
33891704	10	12	theme	carbohydrate-dependent	1437:1458	arg1	manner					1460:1465	a carbohydrate-dependent manner	1435:1465	a carbohydrate-dependent manner with affinity constants in the physiological concentration range	1435:1530	We further showed that hemocyanins bind to human MR and DC-SIGN in a carbohydrate-dependent manner with affinity constants in the physiological concentration range.
33891704	2	13	with	molecules	343:351	arg1	structures					392:401	abundant and complex mannose-rich structures	358:401	abundant and complex mannose-rich structures	358:401	They are multiligand glycosylated molecules with abundant and complex mannose-rich structures.
33891704	3	14	from	processes	495:503	arg1	APCs					514:517	human APCs	508:517	human APCs	508:517	It remains unclear whether these structures influence hemocyanin-induced immunostimulatory processes in human APCs.
33891704	5	15	theme	DC-specific	949:959	arg1	DC-SIGN					940:946	DC-SIGN	940:946	DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin)	940:988	Here, we studied the interactions of these hemocyanins with two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin).
33891704	5	15	theme	DC-specific	949:959	arg1	nonintegrin					977:987	DC-specific ICAM-3-grabbing nonintegrin	949:987	DC-specific ICAM-3-grabbing nonintegrin	949:987	Here, we studied the interactions of these hemocyanins with two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin).
33891704	0	16	theme	immunostimulatory	97:113	arg1	effects					115:121	their immunostimulatory effects	91:121	their immunostimulatory effects	91:121	C-type lectin receptors MR and DC-SIGN are involved in recognition of hemocyanins, shaping their immunostimulatory effects on human dendritic cells.
33891704	4	17	theme	C-type	760:765	arg1	CLRs					785:788	CLRs	785:788	CLRs	785:788	We have previously shown that hemocyanin glycans from Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), participate in their immune recognition and immunogenicity in mice, interacting with murine C-type lectin receptors (CLRs).
33891704	4	17	theme	C-type	760:765	arg1	receptors					774:782	murine C-type lectin receptors	753:782	murine C-type lectin receptors (CLRs)	753:789	We have previously shown that hemocyanin glycans from Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), participate in their immune recognition and immunogenicity in mice, interacting with murine C-type lectin receptors (CLRs).
33891704	5	18	theme	hemocyanins	835:845	arg1	interactions					813:824	the interactions	809:824	the interactions of these hemocyanins with two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin)	809:988	Here, we studied the interactions of these hemocyanins with two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin).
33891704	4	19	from	immunogenicity	712:725	arg1	mice					730:733	mice	730:733	mice	730:733	We have previously shown that hemocyanin glycans from Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), participate in their immune recognition and immunogenicity in mice, interacting with murine C-type lectin receptors (CLRs).
33891704	3	20	theme	human	508:512	arg1	APCs					514:517	human APCs	508:517	human APCs	508:517	It remains unclear whether these structures influence hemocyanin-induced immunostimulatory processes in human APCs.
33891704	10	21	with	manner	1460:1465	arg1	constants					1481:1489	affinity constants	1472:1489	affinity constants in the physiological concentration range	1472:1530	We further showed that hemocyanins bind to human MR and DC-SIGN in a carbohydrate-dependent manner with affinity constants in the physiological concentration range.
33891704	11	22	theme	Th1	1691:1693	arg1	potential					1708:1716	a Th1 cell-driving potential	1689:1716	a Th1 cell-driving potential	1689:1716	Overall, we showed that these three clinically valuable hemocyanins interact with human mannose-sensitive CLRs, initiating an immune response and promoting a Th1 cell-driving potential.
33891704	4	23	dep	mice	730:733	arg1	interacting					736:746	interacting	736:746	interacting with murine C-type lectin receptors (CLRs)	736:789	We have previously shown that hemocyanin glycans from Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), participate in their immune recognition and immunogenicity in mice, interacting with murine C-type lectin receptors (CLRs).
33891704	0	24	theme	lectin	7:12	arg1	receptors					14:22	C-type lectin receptors	0:22	C-type lectin receptors MR and DC-SIGN	0:37	C-type lectin receptors MR and DC-SIGN are involved in recognition of hemocyanins, shaping their immunostimulatory effects on human dendritic cells.
33891704	0	24	theme	lectin	7:12	arg1	DC-SIGN					31:37	DC-SIGN	31:37	DC-SIGN	31:37	C-type lectin receptors MR and DC-SIGN are involved in recognition of hemocyanins, shaping their immunostimulatory effects on human dendritic cells.
33891704	0	24	theme	lectin	7:12	arg1	MR					24:25	MR	24:25	MR	24:25	C-type lectin receptors MR and DC-SIGN are involved in recognition of hemocyanins, shaping their immunostimulatory effects on human dendritic cells.
33891704	4	25	theme	hemocyanin	550:559	arg1	glycans					561:567	hemocyanin glycans	550:567	hemocyanin glycans	550:567	We have previously shown that hemocyanin glycans from Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), participate in their immune recognition and immunogenicity in mice, interacting with murine C-type lectin receptors (CLRs).
33891704	11	26	theme	cell-driving	1695:1706	arg1	potential					1708:1716	a Th1 cell-driving potential	1689:1716	a Th1 cell-driving potential	1689:1716	Overall, we showed that these three clinically valuable hemocyanins interact with human mannose-sensitive CLRs, initiating an immune response and promoting a Th1 cell-driving potential.
33891704	5	27	theme	ICAM-3-grabbing	961:975	arg1	DC-SIGN					940:946	DC-SIGN	940:946	DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin)	940:988	Here, we studied the interactions of these hemocyanins with two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin).
33891704	5	27	theme	ICAM-3-grabbing	961:975	arg1	nonintegrin					977:987	DC-specific ICAM-3-grabbing nonintegrin	949:987	DC-specific ICAM-3-grabbing nonintegrin	949:987	Here, we studied the interactions of these hemocyanins with two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin).
33891704	4	28	from	recognition	696:706	arg1	mice					730:733	mice	730:733	mice	730:733	We have previously shown that hemocyanin glycans from Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), participate in their immune recognition and immunogenicity in mice, interacting with murine C-type lectin receptors (CLRs).
33891704	0	29	theme	C-type	0:5	arg1	receptors					14:22	C-type lectin receptors	0:22	C-type lectin receptors MR and DC-SIGN	0:37	C-type lectin receptors MR and DC-SIGN are involved in recognition of hemocyanins, shaping their immunostimulatory effects on human dendritic cells.
33891704	0	29	theme	C-type	0:5	arg1	DC-SIGN					31:37	DC-SIGN	31:37	DC-SIGN	31:37	C-type lectin receptors MR and DC-SIGN are involved in recognition of hemocyanins, shaping their immunostimulatory effects on human dendritic cells.
33891704	0	29	theme	C-type	0:5	arg1	MR					24:25	MR	24:25	MR	24:25	C-type lectin receptors MR and DC-SIGN are involved in recognition of hemocyanins, shaping their immunostimulatory effects on human dendritic cells.
33891704	1	30	theme	beneficial	289:298	arg1	effects					300:306	beneficial effects	289:306	beneficial effects	289:306	Hemocyanins are used as immunomodulators in clinical applications because they induce a strong Th1-biased cell-mediated immunity, which has beneficial effects.
33891704	5	31	theme	human	903:907	arg1	DCs					909:911	monocyte-derived human DCs	886:911	monocyte-derived human DCs	886:911	Here, we studied the interactions of these hemocyanins with two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin).
33891704	2	32	theme	glycosylated	330:341	arg1	They					309:312	They	309:312	They	309:312	They are multiligand glycosylated molecules with abundant and complex mannose-rich structures.
33891704	2	32	theme	glycosylated	330:341	arg1	molecules					343:351	multiligand glycosylated molecules	318:351	multiligand glycosylated molecules with abundant and complex mannose-rich structures	318:401	They are multiligand glycosylated molecules with abundant and complex mannose-rich structures.
33891704	5	33	link	monocyte-derived	886:901	arg1	DCs					909:911	monocyte-derived human DCs	886:911	monocyte-derived human DCs	886:911	Here, we studied the interactions of these hemocyanins with two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin).
33891704	9	34	theme	deglycosylated	1324:1337	arg1	FLH					1339:1341	deglycosylated FLH	1324:1341	deglycosylated FLH	1324:1341	The hemocyanin-mediated proinflammatory cytokine response was impaired when using deglycosylated FLH and KLH compared to CCH.
33891704	2	35	theme	multiligand	318:328	arg1	They					309:312	They	309:312	They	309:312	They are multiligand glycosylated molecules with abundant and complex mannose-rich structures.
33891704	2	35	theme	multiligand	318:328	arg1	molecules					343:351	multiligand glycosylated molecules	318:351	multiligand glycosylated molecules with abundant and complex mannose-rich structures	318:401	They are multiligand glycosylated molecules with abundant and complex mannose-rich structures.
33891704	10	36	theme	physiological	1498:1510	arg1	range					1526:1530	the physiological concentration range	1494:1530	the physiological concentration range	1494:1530	We further showed that hemocyanins bind to human MR and DC-SIGN in a carbohydrate-dependent manner with affinity constants in the physiological concentration range.
33891704	1	37	theme	clinical	193:200	arg1	applications					202:213	clinical applications	193:213	clinical applications because they induce a strong Th1-biased cell-mediated immunity, which has beneficial effects	193:306	Hemocyanins are used as immunomodulators in clinical applications because they induce a strong Th1-biased cell-mediated immunity, which has beneficial effects.
33891704	4	38	from	latimarginata	616:628	arg1	glycans					561:567	hemocyanin glycans	550:567	hemocyanin glycans	550:567	We have previously shown that hemocyanin glycans from Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), participate in their immune recognition and immunogenicity in mice, interacting with murine C-type lectin receptors (CLRs).
33891704	3	39	theme	hemocyanin-induced	458:475	arg1	processes					495:503	hemocyanin-induced immunostimulatory processes	458:503	hemocyanin-induced immunostimulatory processes in human APCs	458:517	It remains unclear whether these structures influence hemocyanin-induced immunostimulatory processes in human APCs.
33891704	6	40	theme	Diverse	991:997	arg1	analyses					999:1006	Diverse analyses	991:1006	Diverse analyses	991:1006	Diverse analyses showed that hemocyanins are internalized by a mannose-sensitive mechanism.
33891704	0	41	theme	dendritic	132:140	arg1	cells					142:146	human dendritic cells	126:146	human dendritic cells	126:146	C-type lectin receptors MR and DC-SIGN are involved in recognition of hemocyanins, shaping their immunostimulatory effects on human dendritic cells.
33891704	4	42	theme	immune	689:694	arg1	recognition					696:706	immune recognition	689:706	immune recognition	689:706	We have previously shown that hemocyanin glycans from Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), participate in their immune recognition and immunogenicity in mice, interacting with murine C-type lectin receptors (CLRs).
33891704	3	43	theme	immunostimulatory	477:493	arg1	processes					495:503	hemocyanin-induced immunostimulatory processes	458:503	hemocyanin-induced immunostimulatory processes in human APCs	458:517	It remains unclear whether these structures influence hemocyanin-induced immunostimulatory processes in human APCs.
33891704	0	44	theme	human	126:130	arg1	cells					142:146	human dendritic cells	126:146	human dendritic cells	126:146	C-type lectin receptors MR and DC-SIGN are involved in recognition of hemocyanins, shaping their immunostimulatory effects on human dendritic cells.
33891704	6	45	theme	mannose-sensitive	1054:1070	arg1	mechanism					1072:1080	a mannose-sensitive mechanism	1052:1080	a mannose-sensitive mechanism	1052:1080	Diverse analyses showed that hemocyanins are internalized by a mannose-sensitive mechanism.
33891704	5	46	theme	monocyte-derived	886:901	arg1	DCs					909:911	monocyte-derived human DCs	886:911	monocyte-derived human DCs	886:911	Here, we studied the interactions of these hemocyanins with two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin).
33891704	9	47	gly	deglycosylated	1324:1337	arg1	FLH					1339:1341	deglycosylated FLH	1324:1341	deglycosylated FLH	1324:1341	The hemocyanin-mediated proinflammatory cytokine response was impaired when using deglycosylated FLH and KLH compared to CCH.
33891704	5	48	theme	major	856:860	arg1	DC-SIGN					940:946	DC-SIGN	940:946	DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin)	940:988	Here, we studied the interactions of these hemocyanins with two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin).
33891704	5	48	theme	major	856:860	arg1	CLRs					878:881	two major mannose-binding CLRs	852:881	two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin)	852:988	Here, we studied the interactions of these hemocyanins with two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin).
33891704	5	48	theme	major	856:860	arg1	MR					914:915	MR	914:915	MR (mannose receptor)	914:934	Here, we studied the interactions of these hemocyanins with two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin).
33891704	10	49	theme	affinity	1472:1479	arg1	constants					1481:1489	affinity constants	1472:1489	affinity constants in the physiological concentration range	1472:1530	We further showed that hemocyanins bind to human MR and DC-SIGN in a carbohydrate-dependent manner with affinity constants in the physiological concentration range.
33891704	2	50	theme	complex	371:377	arg1	structures					392:401	abundant and complex mannose-rich structures	358:401	abundant and complex mannose-rich structures	358:401	They are multiligand glycosylated molecules with abundant and complex mannose-rich structures.
33891704	4	51	theme	Fissurella	605:614	arg1	FLH					631:633	FLH	631:633	FLH	631:633	We have previously shown that hemocyanin glycans from Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), participate in their immune recognition and immunogenicity in mice, interacting with murine C-type lectin receptors (CLRs).
33891704	4	51	theme	Fissurella	605:614	arg1	latimarginata					616:628	Fissurella latimarginata	605:628	Fissurella latimarginata (FLH)	605:634	We have previously shown that hemocyanin glycans from Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), participate in their immune recognition and immunogenicity in mice, interacting with murine C-type lectin receptors (CLRs).
33891704	5	52	dep	CLRs	878:881	arg1	receptor					926:933	mannose receptor	918:933	mannose receptor	918:933	Here, we studied the interactions of these hemocyanins with two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin).
33891704	5	52	dep	CLRs	878:881	arg1	DC-SIGN					940:946	DC-SIGN	940:946	DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin)	940:988	Here, we studied the interactions of these hemocyanins with two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin).
33891704	5	52	dep	CLRs	878:881	arg1	CLRs					878:881	two major mannose-binding CLRs	852:881	two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin)	852:988	Here, we studied the interactions of these hemocyanins with two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin).
33891704	5	52	dep	CLRs	878:881	arg1	nonintegrin					977:987	DC-specific ICAM-3-grabbing nonintegrin	949:987	DC-specific ICAM-3-grabbing nonintegrin	949:987	Here, we studied the interactions of these hemocyanins with two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin).
33891704	5	52	dep	CLRs	878:881	arg1	MR					914:915	MR	914:915	MR (mannose receptor)	914:934	Here, we studied the interactions of these hemocyanins with two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin).
33891704	11	53	theme	immune	1659:1664	arg1	response					1666:1673	an immune response	1656:1673	an immune response	1656:1673	Overall, we showed that these three clinically valuable hemocyanins interact with human mannose-sensitive CLRs, initiating an immune response and promoting a Th1 cell-driving potential.
33891704	4	54	theme	murine	753:758	arg1	CLRs					785:788	CLRs	785:788	CLRs	785:788	We have previously shown that hemocyanin glycans from Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), participate in their immune recognition and immunogenicity in mice, interacting with murine C-type lectin receptors (CLRs).
33891704	4	54	theme	murine	753:758	arg1	receptors					774:782	murine C-type lectin receptors	753:782	murine C-type lectin receptors (CLRs)	753:789	We have previously shown that hemocyanin glycans from Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), participate in their immune recognition and immunogenicity in mice, interacting with murine C-type lectin receptors (CLRs).
33891704	1	55	used	used	165:168	arg2	Hemocyanins					149:159	Hemocyanins	149:159	Hemocyanins	149:159	Hemocyanins are used as immunomodulators in clinical applications because they induce a strong Th1-biased cell-mediated immunity, which has beneficial effects.
33891704	1	55	used	used	165:168	arg2	immunomodulators					173:188	immunomodulators	173:188	immunomodulators in clinical applications because they induce a strong Th1-biased cell-mediated immunity, which has beneficial effects	173:306	Hemocyanins are used as immunomodulators in clinical applications because they induce a strong Th1-biased cell-mediated immunity, which has beneficial effects.
33891704	1	56	contain	has	285:287	arg2	effects					300:306	beneficial effects	289:306	beneficial effects	289:306	Hemocyanins are used as immunomodulators in clinical applications because they induce a strong Th1-biased cell-mediated immunity, which has beneficial effects.
33891704	1	56	contain	has	285:287	arg1	cell-mediated immunity					255:276	a strong Th1-biased cell-mediated immunity	235:276	a strong Th1-biased cell-mediated immunity	235:276	Hemocyanins are used as immunomodulators in clinical applications because they induce a strong Th1-biased cell-mediated immunity, which has beneficial effects.
33891704	5	57	from	CLRs	878:881	arg1	DCs					909:911	monocyte-derived human DCs	886:911	monocyte-derived human DCs	886:911	Here, we studied the interactions of these hemocyanins with two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin).
33891704	2	58	theme	abundant	358:365	arg1	structures					392:401	abundant and complex mannose-rich structures	358:401	abundant and complex mannose-rich structures	358:401	They are multiligand glycosylated molecules with abundant and complex mannose-rich structures.
33891704	11	59	theme	human	1615:1619	arg1	CLRs					1639:1642	human mannose-sensitive CLRs	1615:1642	human mannose-sensitive CLRs	1615:1642	Overall, we showed that these three clinically valuable hemocyanins interact with human mannose-sensitive CLRs, initiating an immune response and promoting a Th1 cell-driving potential.
33891704	5	60	with	interactions	813:824	arg1	DC-SIGN					940:946	DC-SIGN	940:946	DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin)	940:988	Here, we studied the interactions of these hemocyanins with two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin).
33891704	5	60	with	interactions	813:824	arg1	CLRs					878:881	two major mannose-binding CLRs	852:881	two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin)	852:988	Here, we studied the interactions of these hemocyanins with two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin).
33891704	5	60	with	interactions	813:824	arg1	MR					914:915	MR	914:915	MR (mannose receptor)	914:934	Here, we studied the interactions of these hemocyanins with two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin).
33891704	0	61	from	shaping	83:89	arg1	cells					142:146	human dendritic cells	126:146	human dendritic cells	126:146	C-type lectin receptors MR and DC-SIGN are involved in recognition of hemocyanins, shaping their immunostimulatory effects on human dendritic cells.
33891704	0	62	dep	receptors	14:22	arg1	receptors					14:22	C-type lectin receptors	0:22	C-type lectin receptors MR and DC-SIGN	0:37	C-type lectin receptors MR and DC-SIGN are involved in recognition of hemocyanins, shaping their immunostimulatory effects on human dendritic cells.
33891704	0	62	dep	receptors	14:22	arg1	DC-SIGN					31:37	DC-SIGN	31:37	DC-SIGN	31:37	C-type lectin receptors MR and DC-SIGN are involved in recognition of hemocyanins, shaping their immunostimulatory effects on human dendritic cells.
33891704	0	62	dep	receptors	14:22	arg1	MR					24:25	MR	24:25	MR	24:25	C-type lectin receptors MR and DC-SIGN are involved in recognition of hemocyanins, shaping their immunostimulatory effects on human dendritic cells.
33891704	1	63	from	immunomodulators	173:188	arg1	applications					202:213	clinical applications	193:213	clinical applications because they induce a strong Th1-biased cell-mediated immunity, which has beneficial effects	193:306	Hemocyanins are used as immunomodulators in clinical applications because they induce a strong Th1-biased cell-mediated immunity, which has beneficial effects.
33891704	4	64	theme	lectin	767:772	arg1	CLRs					785:788	CLRs	785:788	CLRs	785:788	We have previously shown that hemocyanin glycans from Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), participate in their immune recognition and immunogenicity in mice, interacting with murine C-type lectin receptors (CLRs).
33891704	4	64	theme	lectin	767:772	arg1	receptors					774:782	murine C-type lectin receptors	753:782	murine C-type lectin receptors (CLRs)	753:789	We have previously shown that hemocyanin glycans from Concholepas concholepas (CCH), Fissurella latimarginata (FLH), and Megathura crenulata (KLH), participate in their immune recognition and immunogenicity in mice, interacting with murine C-type lectin receptors (CLRs).
33891704	2	65	gly	glycosylated	330:341	arg1	They					309:312	They	309:312	They	309:312	They are multiligand glycosylated molecules with abundant and complex mannose-rich structures.
33891704	2	65	gly	glycosylated	330:341	arg1	molecules					343:351	multiligand glycosylated molecules	318:351	multiligand glycosylated molecules with abundant and complex mannose-rich structures	318:401	They are multiligand glycosylated molecules with abundant and complex mannose-rich structures.
33891704	5	66	theme	mannose-binding	862:876	arg1	DC-SIGN					940:946	DC-SIGN	940:946	DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin)	940:988	Here, we studied the interactions of these hemocyanins with two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin).
33891704	5	66	theme	mannose-binding	862:876	arg1	CLRs					878:881	two major mannose-binding CLRs	852:881	two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin)	852:988	Here, we studied the interactions of these hemocyanins with two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin).
33891704	5	66	theme	mannose-binding	862:876	arg1	MR					914:915	MR	914:915	MR (mannose receptor)	914:934	Here, we studied the interactions of these hemocyanins with two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin).
33891704	1	67	theme	strong	237:242	arg1	cell-mediated immunity					255:276	a strong Th1-biased cell-mediated immunity	235:276	a strong Th1-biased cell-mediated immunity	235:276	Hemocyanins are used as immunomodulators in clinical applications because they induce a strong Th1-biased cell-mediated immunity, which has beneficial effects.
33891704	10	68	theme	concentration	1512:1524	arg1	range					1526:1530	the physiological concentration range	1494:1530	the physiological concentration range	1494:1530	We further showed that hemocyanins bind to human MR and DC-SIGN in a carbohydrate-dependent manner with affinity constants in the physiological concentration range.
33891704	1	69	dep	applications	202:213	arg1	induce					228:233	induce	228:233	induce a strong Th1-biased cell-mediated immunity, which has beneficial effects	228:306	Hemocyanins are used as immunomodulators in clinical applications because they induce a strong Th1-biased cell-mediated immunity, which has beneficial effects.
33891704	5	70	theme	mannose	918:924	arg1	receptor					926:933	mannose receptor	918:933	mannose receptor	918:933	Here, we studied the interactions of these hemocyanins with two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin).
33891704	5	70	theme	mannose	918:924	arg1	MR					914:915	MR	914:915	MR (mannose receptor)	914:934	Here, we studied the interactions of these hemocyanins with two major mannose-binding CLRs on monocyte-derived human DCs: MR (mannose receptor) and DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin).
33891704	1	71	theme	Th1-biased	244:253	arg1	cell-mediated immunity					255:276	a strong Th1-biased cell-mediated immunity	235:276	a strong Th1-biased cell-mediated immunity	235:276	Hemocyanins are used as immunomodulators in clinical applications because they induce a strong Th1-biased cell-mediated immunity, which has beneficial effects.
33627655	5	0	theme	single	767:772	arg1	cell					774:777	single cell	767:777	single cell	767:777	Analysing the myeloid composition in PDAC, using single cell and bulk transcriptomics data, we identified monocyte-derived macrophages as contributors to the poor clinical outcome.
33627655	1	1	theme	tumour	171:176	arg1	progression					178:188	tumour progression	171:188	tumour progression	171:188	Changes in glycosylation during tumour progression are a key hallmark of cancer.
33627655	4	2	theme	α2,3	583:586	arg1	sialyltransferases					588:605	the α2,3 sialyltransferases ST3GAL1 and ST3GAL4	579:625	the α2,3 sialyltransferases ST3GAL1 and ST3GAL4	579:625	We identified the expression of the α2,3 sialyltransferases ST3GAL1 and ST3GAL4 as main contributor to the synthesis of ligands for Siglec-7 and Siglec-9 in tumour cells.
33627655	4	2	theme	α2,3	583:586	arg1	ST3GAL4					619:625	ST3GAL4	619:625	ST3GAL4	619:625	We identified the expression of the α2,3 sialyltransferases ST3GAL1 and ST3GAL4 as main contributor to the synthesis of ligands for Siglec-7 and Siglec-9 in tumour cells.
33627655	4	2	theme	α2,3	583:586	arg1	ST3GAL1					607:613	ST3GAL1	607:613	ST3GAL1	607:613	We identified the expression of the α2,3 sialyltransferases ST3GAL1 and ST3GAL4 as main contributor to the synthesis of ligands for Siglec-7 and Siglec-9 in tumour cells.
33627655	5	3	theme	cell	774:777	arg1	data					804:807	single cell and bulk transcriptomics data	767:807	single cell and bulk transcriptomics data	767:807	Analysing the myeloid composition in PDAC, using single cell and bulk transcriptomics data, we identified monocyte-derived macrophages as contributors to the poor clinical outcome.
33627655	0	4	from	acids	7:11	arg1	cells					34:38	pancreatic cancer cells	16:38	pancreatic cancer cells	16:38	Sialic acids in pancreatic cancer cells drive tumour-associated macrophage differentiation via the Siglec receptors Siglec-7 and Siglec-9.
33627655	7	5	theme	different	1185:1193	arg1	cells					1203:1207	different myeloid cells	1185:1207	different myeloid cells	1185:1207	Moreover, triggering of Siglec-9 in macrophages reduce inflammatory programmes, while increasing PD-L1 and IL-10 expression, illustrating that sialic acids modulate different myeloid cells.
33627655	3	6	from	Siglec-7	507:514	arg1	cells					540:544	myeloid cells	532:544	myeloid cells	532:544	We here show that Pancreatic Ductal Adenocarcinoma (PDAC) tumour cells present an increased sialylation that can be recognized by Siglec-7 and Siglec-9 on myeloid cells.
33627655	2	7	theme	Siglec	359:364	arg1	receptors					366:374	Siglec receptors	359:374	Siglec receptors	359:374	One of the glycan moieties generally overexpressed in cancer are sialic acids, which can induce immunomodulatory properties via binding to Siglec receptors.
33627655	4	8	from	Siglec-9	692:699	arg1	cells					711:715	tumour cells	704:715	tumour cells	704:715	We identified the expression of the α2,3 sialyltransferases ST3GAL1 and ST3GAL4 as main contributor to the synthesis of ligands for Siglec-7 and Siglec-9 in tumour cells.
33627655	5	9	theme	myeloid	732:738	arg1	composition					740:750	the myeloid composition	728:750	the myeloid composition in PDAC, using single cell and bulk transcriptomics data	728:807	Analysing the myeloid composition in PDAC, using single cell and bulk transcriptomics data, we identified monocyte-derived macrophages as contributors to the poor clinical outcome.
33627655	7	10	theme	sialic	1163:1168	arg1	acids					1170:1174	sialic acids	1163:1174	sialic acids	1163:1174	Moreover, triggering of Siglec-9 in macrophages reduce inflammatory programmes, while increasing PD-L1 and IL-10 expression, illustrating that sialic acids modulate different myeloid cells.
33627655	8	11	gly	sialylated	1251:1260	arg1	glycans					1262:1268	sialylated glycans	1251:1268	sialylated glycans	1251:1268	This work highlights a critical role for sialylated glycans in controlling immune suppression and provides new potential targets for cancer immunotherapy in PDAC.
33627655	0	12	theme	Siglec	99:104	arg1	receptors					106:114	the Siglec receptors Siglec-7 and Siglec-9	95:136	the Siglec receptors Siglec-7 and Siglec-9	95:136	Sialic acids in pancreatic cancer cells drive tumour-associated macrophage differentiation via the Siglec receptors Siglec-7 and Siglec-9.
33627655	0	12	theme	Siglec	99:104	arg1	Siglec-9					129:136	Siglec-9	129:136	Siglec-9	129:136	Sialic acids in pancreatic cancer cells drive tumour-associated macrophage differentiation via the Siglec receptors Siglec-7 and Siglec-9.
33627655	0	12	theme	Siglec	99:104	arg1	Siglec-7					116:123	Siglec-7	116:123	Siglec-7	116:123	Sialic acids in pancreatic cancer cells drive tumour-associated macrophage differentiation via the Siglec receptors Siglec-7 and Siglec-9.
33627655	3	13	from	Siglec-9	520:527	arg1	cells					540:544	myeloid cells	532:544	myeloid cells	532:544	We here show that Pancreatic Ductal Adenocarcinoma (PDAC) tumour cells present an increased sialylation that can be recognized by Siglec-7 and Siglec-9 on myeloid cells.
33627655	4	14	theme	tumour	704:709	arg1	cells					711:715	tumour cells	704:715	tumour cells	704:715	We identified the expression of the α2,3 sialyltransferases ST3GAL1 and ST3GAL4 as main contributor to the synthesis of ligands for Siglec-7 and Siglec-9 in tumour cells.
33627655	4	15	from	Siglec-7	679:686	arg1	cells					711:715	tumour cells	704:715	tumour cells	704:715	We identified the expression of the α2,3 sialyltransferases ST3GAL1 and ST3GAL4 as main contributor to the synthesis of ligands for Siglec-7 and Siglec-9 in tumour cells.
33627655	4	16	theme	sialyltransferases	588:605	arg1	expression					565:574	the expression	561:574	the expression of the α2,3 sialyltransferases ST3GAL1 and ST3GAL4	561:625	We identified the expression of the α2,3 sialyltransferases ST3GAL1 and ST3GAL4 as main contributor to the synthesis of ligands for Siglec-7 and Siglec-9 in tumour cells.
33627655	8	17	theme	critical	1233:1240	arg1	role					1242:1245	a critical role	1231:1245	a critical role for sialylated glycans in controlling immune suppression	1231:1302	This work highlights a critical role for sialylated glycans in controlling immune suppression and provides new potential targets for cancer immunotherapy in PDAC.
33627655	6	18	theme	sialic	914:919	arg1	acids					921:925	Tumour-derived sialic acids	899:925	Tumour-derived sialic acids	899:925	Tumour-derived sialic acids dictate monocyte to macrophage differentiation via signalling through Siglec-7 and Siglec-9.
33627655	0	19	dep	receptors	106:114	arg1	receptors					106:114	the Siglec receptors Siglec-7 and Siglec-9	95:136	the Siglec receptors Siglec-7 and Siglec-9	95:136	Sialic acids in pancreatic cancer cells drive tumour-associated macrophage differentiation via the Siglec receptors Siglec-7 and Siglec-9.
33627655	0	19	dep	receptors	106:114	arg1	Siglec-9					129:136	Siglec-9	129:136	Siglec-9	129:136	Sialic acids in pancreatic cancer cells drive tumour-associated macrophage differentiation via the Siglec receptors Siglec-7 and Siglec-9.
33627655	0	19	dep	receptors	106:114	arg1	Siglec-7					116:123	Siglec-7	116:123	Siglec-7	116:123	Sialic acids in pancreatic cancer cells drive tumour-associated macrophage differentiation via the Siglec receptors Siglec-7 and Siglec-9.
33627655	5	20	theme	clinical	881:888	arg1	outcome					890:896	the poor clinical outcome	872:896	the poor clinical outcome	872:896	Analysing the myeloid composition in PDAC, using single cell and bulk transcriptomics data, we identified monocyte-derived macrophages as contributors to the poor clinical outcome.
33627655	4	21	theme	ligands	667:673	arg1	synthesis					654:662	the synthesis	650:662	the synthesis of ligands for Siglec-7 and Siglec-9 in tumour cells	650:715	We identified the expression of the α2,3 sialyltransferases ST3GAL1 and ST3GAL4 as main contributor to the synthesis of ligands for Siglec-7 and Siglec-9 in tumour cells.
33627655	8	22	theme	immune	1285:1290	arg1	suppression					1292:1302	immune suppression	1285:1302	immune suppression	1285:1302	This work highlights a critical role for sialylated glycans in controlling immune suppression and provides new potential targets for cancer immunotherapy in PDAC.
33627655	6	23	theme	Tumour-derived	899:912	arg1	acids					921:925	Tumour-derived sialic acids	899:925	Tumour-derived sialic acids	899:925	Tumour-derived sialic acids dictate monocyte to macrophage differentiation via signalling through Siglec-7 and Siglec-9.
33627655	6	24	dep	monocyte	935:942	arg1	to					944:945	to	944:945	to	944:945	Tumour-derived sialic acids dictate monocyte to macrophage differentiation via signalling through Siglec-7 and Siglec-9.
33627655	6	24	dep	monocyte	935:942	arg1	macrophage					947:956	macrophage	947:956	monocyte to macrophage differentiation	935:972	Tumour-derived sialic acids dictate monocyte to macrophage differentiation via signalling through Siglec-7 and Siglec-9.
33627655	1	25	from	Changes	139:145	arg1	glycosylation					150:162	glycosylation	150:162	glycosylation	150:162	Changes in glycosylation during tumour progression are a key hallmark of cancer.
33627655	3	26	theme	Pancreatic	395:404	arg1	Adenocarcinoma					413:426	Pancreatic Ductal Adenocarcinoma	395:426	Pancreatic Ductal Adenocarcinoma (PDAC) tumour cells	395:446	We here show that Pancreatic Ductal Adenocarcinoma (PDAC) tumour cells present an increased sialylation that can be recognized by Siglec-7 and Siglec-9 on myeloid cells.
33627655	3	26	theme	Pancreatic	395:404	arg1	PDAC					429:432	PDAC	429:432	PDAC	429:432	We here show that Pancreatic Ductal Adenocarcinoma (PDAC) tumour cells present an increased sialylation that can be recognized by Siglec-7 and Siglec-9 on myeloid cells.
33627655	5	27	theme	poor	876:879	arg1	outcome					890:896	the poor clinical outcome	872:896	the poor clinical outcome	872:896	Analysing the myeloid composition in PDAC, using single cell and bulk transcriptomics data, we identified monocyte-derived macrophages as contributors to the poor clinical outcome.
33627655	2	28	theme	immunomodulatory	316:331	arg1	properties					333:342	immunomodulatory properties	316:342	immunomodulatory properties	316:342	One of the glycan moieties generally overexpressed in cancer are sialic acids, which can induce immunomodulatory properties via binding to Siglec receptors.
33627655	3	29	theme	myeloid	532:538	arg1	cells					540:544	myeloid cells	532:544	myeloid cells	532:544	We here show that Pancreatic Ductal Adenocarcinoma (PDAC) tumour cells present an increased sialylation that can be recognized by Siglec-7 and Siglec-9 on myeloid cells.
33627655	1	30	theme	key	196:198	arg1	Changes					139:145	Changes	139:145	Changes in glycosylation during tumour progression	139:188	Changes in glycosylation during tumour progression are a key hallmark of cancer.
33627655	1	30	theme	key	196:198	arg1	hallmark					200:207	a key hallmark	194:207	a key hallmark of cancer	194:217	Changes in glycosylation during tumour progression are a key hallmark of cancer.
33627655	0	31	theme	Sialic	0:5	arg1	acids					7:11	Sialic acids	0:11	Sialic acids in pancreatic cancer cells	0:38	Sialic acids in pancreatic cancer cells drive tumour-associated macrophage differentiation via the Siglec receptors Siglec-7 and Siglec-9.
33627655	3	32	theme	tumour	435:440	arg1	cells					442:446	Pancreatic Ductal Adenocarcinoma (PDAC) tumour cells	395:446	Pancreatic Ductal Adenocarcinoma (PDAC) tumour cells	395:446	We here show that Pancreatic Ductal Adenocarcinoma (PDAC) tumour cells present an increased sialylation that can be recognized by Siglec-7 and Siglec-9 on myeloid cells.
33627655	0	33	theme	pancreatic	16:25	arg1	cells					34:38	pancreatic cancer cells	16:38	pancreatic cancer cells	16:38	Sialic acids in pancreatic cancer cells drive tumour-associated macrophage differentiation via the Siglec receptors Siglec-7 and Siglec-9.
33627655	5	34	theme	transcriptomics	788:802	arg1	data					804:807	single cell and bulk transcriptomics data	767:807	single cell and bulk transcriptomics data	767:807	Analysing the myeloid composition in PDAC, using single cell and bulk transcriptomics data, we identified monocyte-derived macrophages as contributors to the poor clinical outcome.
33627655	6	35	link	Tumour-derived	899:912	arg1	acids					921:925	Tumour-derived sialic acids	899:925	Tumour-derived sialic acids	899:925	Tumour-derived sialic acids dictate monocyte to macrophage differentiation via signalling through Siglec-7 and Siglec-9.
33627655	5	36	theme	monocyte-derived	824:839	arg1	contributors					856:867	contributors	856:867	contributors to the poor clinical outcome	856:896	Analysing the myeloid composition in PDAC, using single cell and bulk transcriptomics data, we identified monocyte-derived macrophages as contributors to the poor clinical outcome.
33627655	5	36	theme	monocyte-derived	824:839	arg1	macrophages					841:851	monocyte-derived macrophages	824:851	monocyte-derived macrophages	824:851	Analysing the myeloid composition in PDAC, using single cell and bulk transcriptomics data, we identified monocyte-derived macrophages as contributors to the poor clinical outcome.
33627655	2	37	theme	sialic	285:290	arg1	acids					292:296	sialic acids	285:296	sialic acids	285:296	One of the glycan moieties generally overexpressed in cancer are sialic acids, which can induce immunomodulatory properties via binding to Siglec receptors.
33627655	2	37	theme	sialic	285:290	arg1	moieties					238:245	the glycan moieties	227:245	the glycan moieties generally overexpressed in cancer	227:279	One of the glycan moieties generally overexpressed in cancer are sialic acids, which can induce immunomodulatory properties via binding to Siglec receptors.
33627655	2	37	theme	sialic	285:290	arg1	One					220:222	One	220:222	One	220:222	One of the glycan moieties generally overexpressed in cancer are sialic acids, which can induce immunomodulatory properties via binding to Siglec receptors.
33627655	5	38	from	composition	740:750	arg1	PDAC					755:758	PDAC	755:758	PDAC	755:758	Analysing the myeloid composition in PDAC, using single cell and bulk transcriptomics data, we identified monocyte-derived macrophages as contributors to the poor clinical outcome.
33627655	1	39	theme	cancer	212:217	arg1	Changes					139:145	Changes	139:145	Changes in glycosylation during tumour progression	139:188	Changes in glycosylation during tumour progression are a key hallmark of cancer.
33627655	1	39	theme	cancer	212:217	arg1	hallmark					200:207	a key hallmark	194:207	a key hallmark of cancer	194:217	Changes in glycosylation during tumour progression are a key hallmark of cancer.
33627655	7	40	dep	PD-L1	1117:1121	arg1	expression					1133:1142	expression	1133:1142	expression	1133:1142	Moreover, triggering of Siglec-9 in macrophages reduce inflammatory programmes, while increasing PD-L1 and IL-10 expression, illustrating that sialic acids modulate different myeloid cells.
33627655	4	41	dep	sialyltransferases	588:605	arg1	sialyltransferases					588:605	the α2,3 sialyltransferases ST3GAL1 and ST3GAL4	579:625	the α2,3 sialyltransferases ST3GAL1 and ST3GAL4	579:625	We identified the expression of the α2,3 sialyltransferases ST3GAL1 and ST3GAL4 as main contributor to the synthesis of ligands for Siglec-7 and Siglec-9 in tumour cells.
33627655	4	41	dep	sialyltransferases	588:605	arg1	ST3GAL4					619:625	ST3GAL4	619:625	ST3GAL4	619:625	We identified the expression of the α2,3 sialyltransferases ST3GAL1 and ST3GAL4 as main contributor to the synthesis of ligands for Siglec-7 and Siglec-9 in tumour cells.
33627655	4	41	dep	sialyltransferases	588:605	arg1	ST3GAL1					607:613	ST3GAL1	607:613	ST3GAL1	607:613	We identified the expression of the α2,3 sialyltransferases ST3GAL1 and ST3GAL4 as main contributor to the synthesis of ligands for Siglec-7 and Siglec-9 in tumour cells.
33627655	2	42	theme	moieties	238:245	arg1	acids					292:296	sialic acids	285:296	sialic acids	285:296	One of the glycan moieties generally overexpressed in cancer are sialic acids, which can induce immunomodulatory properties via binding to Siglec receptors.
33627655	2	42	theme	moieties	238:245	arg1	moieties					238:245	the glycan moieties	227:245	the glycan moieties generally overexpressed in cancer	227:279	One of the glycan moieties generally overexpressed in cancer are sialic acids, which can induce immunomodulatory properties via binding to Siglec receptors.
33627655	2	42	theme	moieties	238:245	arg1	One					220:222	One	220:222	One	220:222	One of the glycan moieties generally overexpressed in cancer are sialic acids, which can induce immunomodulatory properties via binding to Siglec receptors.
33627655	8	43	theme	new	1317:1319	arg1	targets					1331:1337	new potential targets	1317:1337	new potential targets for cancer immunotherapy	1317:1362	This work highlights a critical role for sialylated glycans in controlling immune suppression and provides new potential targets for cancer immunotherapy in PDAC.
33627655	7	44	theme	myeloid	1195:1201	arg1	cells					1203:1207	different myeloid cells	1185:1207	different myeloid cells	1185:1207	Moreover, triggering of Siglec-9 in macrophages reduce inflammatory programmes, while increasing PD-L1 and IL-10 expression, illustrating that sialic acids modulate different myeloid cells.
33627655	0	45	theme	cancer	27:32	arg1	cells					34:38	pancreatic cancer cells	16:38	pancreatic cancer cells	16:38	Sialic acids in pancreatic cancer cells drive tumour-associated macrophage differentiation via the Siglec receptors Siglec-7 and Siglec-9.
33627655	5	46	theme	bulk	783:786	arg1	transcriptomics					788:802	bulk transcriptomics	783:802	bulk transcriptomics	783:802	Analysing the myeloid composition in PDAC, using single cell and bulk transcriptomics data, we identified monocyte-derived macrophages as contributors to the poor clinical outcome.
33627655	2	47	theme	glycan	231:236	arg1	moieties					238:245	the glycan moieties	227:245	the glycan moieties generally overexpressed in cancer	227:279	One of the glycan moieties generally overexpressed in cancer are sialic acids, which can induce immunomodulatory properties via binding to Siglec receptors.
33627655	3	48	theme	increased	459:467	arg1	sialylation					469:479	an increased sialylation	456:479	an increased sialylation that can be recognized by Siglec-7 and Siglec-9 on myeloid cells	456:544	We here show that Pancreatic Ductal Adenocarcinoma (PDAC) tumour cells present an increased sialylation that can be recognized by Siglec-7 and Siglec-9 on myeloid cells.
33627655	3	49	theme	Ductal	406:411	arg1	Adenocarcinoma					413:426	Pancreatic Ductal Adenocarcinoma	395:426	Pancreatic Ductal Adenocarcinoma (PDAC) tumour cells	395:446	We here show that Pancreatic Ductal Adenocarcinoma (PDAC) tumour cells present an increased sialylation that can be recognized by Siglec-7 and Siglec-9 on myeloid cells.
33627655	3	49	theme	Ductal	406:411	arg1	PDAC					429:432	PDAC	429:432	PDAC	429:432	We here show that Pancreatic Ductal Adenocarcinoma (PDAC) tumour cells present an increased sialylation that can be recognized by Siglec-7 and Siglec-9 on myeloid cells.
33627655	0	50	theme	macrophage	64:73	arg1	differentiation					75:89	tumour-associated macrophage differentiation	46:89	tumour-associated macrophage differentiation	46:89	Sialic acids in pancreatic cancer cells drive tumour-associated macrophage differentiation via the Siglec receptors Siglec-7 and Siglec-9.
33627655	3	51	theme	Adenocarcinoma	413:426	arg1	cells					442:446	Pancreatic Ductal Adenocarcinoma (PDAC) tumour cells	395:446	Pancreatic Ductal Adenocarcinoma (PDAC) tumour cells	395:446	We here show that Pancreatic Ductal Adenocarcinoma (PDAC) tumour cells present an increased sialylation that can be recognized by Siglec-7 and Siglec-9 on myeloid cells.
33627655	6	52	theme	monocyte	935:942	arg1	differentiation					958:972	monocyte to macrophage differentiation	935:972	monocyte to macrophage differentiation	935:972	Tumour-derived sialic acids dictate monocyte to macrophage differentiation via signalling through Siglec-7 and Siglec-9.
33627655	7	53	theme	Siglec-9	1044:1051	arg1	triggering					1030:1039	triggering	1030:1039	triggering of Siglec-9 in macrophages	1030:1066	Moreover, triggering of Siglec-9 in macrophages reduce inflammatory programmes, while increasing PD-L1 and IL-10 expression, illustrating that sialic acids modulate different myeloid cells.
33627655	7	54	theme	inflammatory	1075:1086	arg1	programmes					1088:1097	inflammatory programmes	1075:1097	inflammatory programmes	1075:1097	Moreover, triggering of Siglec-9 in macrophages reduce inflammatory programmes, while increasing PD-L1 and IL-10 expression, illustrating that sialic acids modulate different myeloid cells.
33627655	8	55	theme	potential	1321:1329	arg1	targets					1331:1337	new potential targets	1317:1337	new potential targets for cancer immunotherapy	1317:1362	This work highlights a critical role for sialylated glycans in controlling immune suppression and provides new potential targets for cancer immunotherapy in PDAC.
33627655	5	56	link	monocyte-derived	824:839	arg1	contributors					856:867	contributors	856:867	contributors to the poor clinical outcome	856:896	Analysing the myeloid composition in PDAC, using single cell and bulk transcriptomics data, we identified monocyte-derived macrophages as contributors to the poor clinical outcome.
33627655	5	56	link	monocyte-derived	824:839	arg1	macrophages					841:851	monocyte-derived macrophages	824:851	monocyte-derived macrophages	824:851	Analysing the myeloid composition in PDAC, using single cell and bulk transcriptomics data, we identified monocyte-derived macrophages as contributors to the poor clinical outcome.
33627655	8	57	theme	cancer	1343:1348	arg1	immunotherapy					1350:1362	cancer immunotherapy	1343:1362	cancer immunotherapy	1343:1362	This work highlights a critical role for sialylated glycans in controlling immune suppression and provides new potential targets for cancer immunotherapy in PDAC.
33627655	7	58	from	triggering	1030:1039	arg1	macrophages					1056:1066	macrophages	1056:1066	macrophages	1056:1066	Moreover, triggering of Siglec-9 in macrophages reduce inflammatory programmes, while increasing PD-L1 and IL-10 expression, illustrating that sialic acids modulate different myeloid cells.
33627655	4	59	theme	main	630:633	arg1	contributor					635:645	main contributor	630:645	main contributor to the synthesis of ligands for Siglec-7 and Siglec-9 in tumour cells	630:715	We identified the expression of the α2,3 sialyltransferases ST3GAL1 and ST3GAL4 as main contributor to the synthesis of ligands for Siglec-7 and Siglec-9 in tumour cells.
33627655	0	60	theme	tumour-associated	46:62	arg1	differentiation					75:89	tumour-associated macrophage differentiation	46:89	tumour-associated macrophage differentiation	46:89	Sialic acids in pancreatic cancer cells drive tumour-associated macrophage differentiation via the Siglec receptors Siglec-7 and Siglec-9.
33627655	8	61	theme	sialylated	1251:1260	arg1	glycans					1262:1268	sialylated glycans	1251:1268	sialylated glycans	1251:1268	This work highlights a critical role for sialylated glycans in controlling immune suppression and provides new potential targets for cancer immunotherapy in PDAC.
32880001	5	0	theme	regulatory	699:708	arg1	elements					710:717	the promoter cis-acting regulatory elements	675:717	the promoter cis-acting regulatory elements	675:717	Analysis of the promoter cis-acting regulatory elements and expression responses to different treatments revealed that the Eucalyptus globulus PHI-1 (EgPHI-1) is transcriptionally regulated by auxin, cytokinin, wounding and drought.
32880001	6	1	theme	transgenic	922:931	arg1	tobacco					933:939	transgenic tobacco	922:939	transgenic tobacco	922:939	EgPHI-1 overexpression in transgenic tobacco changed the partitioning of biomass, favoring its allocation to shoots in detriment of roots.
32880001	8	2	from	lignification	1335:1347	arg1	types					1374:1378	both cell types	1364:1378	both cell types	1364:1378	UV microspectrophotometry of individual cell wall layers of fibers and vessels has shown that the transgenic plants exhibit differences in the lignification of S2 layer in both cell types.
32880001	8	3	from	differences	1316:1326	arg1	lignification					1335:1347	the lignification	1331:1347	the lignification of S2 layer in both cell types	1331:1378	UV microspectrophotometry of individual cell wall layers of fibers and vessels has shown that the transgenic plants exhibit differences in the lignification of S2 layer in both cell types.
32880001	7	4	theme	longer	1076:1081	arg1	fibers					1089:1094	longer xylem fibers	1076:1094	longer xylem fibers	1076:1094	The stem of the transgenic plants showed longer xylem fibers and reduced cellulose content, while the leaf xylem had enhanced secondary cell wall thickness.
32880001	8	5	theme	fibers	1252:1257	arg1	layers					1242:1247	individual cell wall layers	1221:1247	individual cell wall layers of fibers and vessels	1221:1269	UV microspectrophotometry of individual cell wall layers of fibers and vessels has shown that the transgenic plants exhibit differences in the lignification of S2 layer in both cell types.
32880001	5	6	theme	expression	723:732	arg1	responses					734:742	expression responses	723:742	expression responses to different treatments	723:766	Analysis of the promoter cis-acting regulatory elements and expression responses to different treatments revealed that the Eucalyptus globulus PHI-1 (EgPHI-1) is transcriptionally regulated by auxin, cytokinin, wounding and drought.
32880001	7	7	theme	reduced	1100:1106	arg1	content					1118:1124	reduced cellulose content	1100:1124	reduced cellulose content	1100:1124	The stem of the transgenic plants showed longer xylem fibers and reduced cellulose content, while the leaf xylem had enhanced secondary cell wall thickness.
32880001	3	8	theme	phenotypes	538:547	arg1	species					515:521	Eucalyptus species	504:521	Eucalyptus species of contrasting phenotypes for wood quality and growth traits	504:582	Here, we report the functional characterization of a PHOSPHATE-INDUCED PROTEIN 1 homologue showing differential expression in xylem cells from Eucalyptus species of contrasting phenotypes for wood quality and growth traits.
32880001	7	9	theme	transgenic	1051:1060	arg1	plants					1062:1067	the transgenic plants	1047:1067	the transgenic plants	1047:1067	The stem of the transgenic plants showed longer xylem fibers and reduced cellulose content, while the leaf xylem had enhanced secondary cell wall thickness.
32880001	0	10	theme	shoot	77:81	arg1	growth					83:88	shoot growth	77:88	shoot growth	77:88	EgPHI-1, a PHOSPHATE-INDUCED-1 gene from Eucalyptus globulus, is involved in shoot growth, xylem fiber length and secondary cell wall properties.
32880001	0	11	from	globulus	52:59	arg1	EgPHI-1					0:6	EgPHI-1	0:6	EgPHI-1	0:6	EgPHI-1, a PHOSPHATE-INDUCED-1 gene from Eucalyptus globulus, is involved in shoot growth, xylem fiber length and secondary cell wall properties.
32880001	0	11	from	globulus	52:59	arg1	gene					31:34	a PHOSPHATE-INDUCED-1 gene	9:34	a PHOSPHATE-INDUCED-1 gene from Eucalyptus globulus	9:59	EgPHI-1, a PHOSPHATE-INDUCED-1 gene from Eucalyptus globulus, is involved in shoot growth, xylem fiber length and secondary cell wall properties.
32880001	9	12	dep	it	1555:1556	arg1	target					1572:1577	an attractive target	1558:1577	it an attractive target for biotechnological applications in forestry and biofuel crops	1555:1641	Taken together, the results suggest that EgPHI-1 mediates the elongation of secondary xylem fibers, secondary cell wall thickening and composition, and lignification, making it an attractive target for biotechnological applications in forestry and biofuel crops.
32880001	8	13	theme	S2	1352:1353	arg1	layer					1355:1359	S2 layer	1352:1359	S2 layer	1352:1359	UV microspectrophotometry of individual cell wall layers of fibers and vessels has shown that the transgenic plants exhibit differences in the lignification of S2 layer in both cell types.
32880001	9	14	theme	fibers	1473:1478	arg1	thickening					1501:1510	secondary cell wall thickening	1481:1510	secondary cell wall thickening	1481:1510	Taken together, the results suggest that EgPHI-1 mediates the elongation of secondary xylem fibers, secondary cell wall thickening and composition, and lignification, making it an attractive target for biotechnological applications in forestry and biofuel crops.
32880001	9	14	theme	fibers	1473:1478	arg1	elongation					1443:1452	the elongation	1439:1452	the elongation of secondary xylem fibers	1439:1478	Taken together, the results suggest that EgPHI-1 mediates the elongation of secondary xylem fibers, secondary cell wall thickening and composition, and lignification, making it an attractive target for biotechnological applications in forestry and biofuel crops.
32880001	9	14	theme	fibers	1473:1478	arg1	composition					1516:1526	composition	1516:1526	composition	1516:1526	Taken together, the results suggest that EgPHI-1 mediates the elongation of secondary xylem fibers, secondary cell wall thickening and composition, and lignification, making it an attractive target for biotechnological applications in forestry and biofuel crops.
32880001	9	14	theme	fibers	1473:1478	arg1	lignification					1533:1545	lignification	1533:1545	lignification	1533:1545	Taken together, the results suggest that EgPHI-1 mediates the elongation of secondary xylem fibers, secondary cell wall thickening and composition, and lignification, making it an attractive target for biotechnological applications in forestry and biofuel crops.
32880001	0	15	theme	xylem	91:95	arg1	length					103:108	xylem fiber length	91:108	xylem fiber length	91:108	EgPHI-1, a PHOSPHATE-INDUCED-1 gene from Eucalyptus globulus, is involved in shoot growth, xylem fiber length and secondary cell wall properties.
32880001	6	16	theme	EgPHI-1	896:902	arg1	overexpression					904:917	EgPHI-1 overexpression	896:917	EgPHI-1 overexpression in transgenic tobacco	896:939	EgPHI-1 overexpression in transgenic tobacco changed the partitioning of biomass, favoring its allocation to shoots in detriment of roots.
32880001	6	17	from	shoots	1005:1010	arg1	detriment					1015:1023	detriment	1015:1023	detriment of roots	1015:1032	EgPHI-1 overexpression in transgenic tobacco changed the partitioning of biomass, favoring its allocation to shoots in detriment of roots.
32880001	3	18	from	species	515:521	arg1	expression					473:482	differential expression	460:482	differential expression in xylem cells from Eucalyptus species of contrasting phenotypes for wood quality and growth traits	460:582	Here, we report the functional characterization of a PHOSPHATE-INDUCED PROTEIN 1 homologue showing differential expression in xylem cells from Eucalyptus species of contrasting phenotypes for wood quality and growth traits.
32880001	3	18	from	species	515:521	arg1	cells					493:497	xylem cells	487:497	xylem cells from Eucalyptus species of contrasting phenotypes for wood quality and growth traits	487:582	Here, we report the functional characterization of a PHOSPHATE-INDUCED PROTEIN 1 homologue showing differential expression in xylem cells from Eucalyptus species of contrasting phenotypes for wood quality and growth traits.
32880001	2	19	theme	biomass	252:258	arg1	partitioning					260:271	biomass partitioning	252:271	biomass partitioning	252:271	Its overexpression in tobacco resulted in changes in biomass partitioning, xylem fiber length, secondary cell wall thickening and composition, and lignification.
32880001	4	20	theme	PHI-1/EXO/EXL	641:653	arg1	family					655:660	the PHI-1/EXO/EXL family	637:660	the PHI-1/EXO/EXL family	637:660	Our results indicated that this gene is a member of the PHI-1/EXO/EXL family.
32880001	5	21	theme	different	747:755	arg1	treatments					757:766	different treatments	747:766	different treatments	747:766	Analysis of the promoter cis-acting regulatory elements and expression responses to different treatments revealed that the Eucalyptus globulus PHI-1 (EgPHI-1) is transcriptionally regulated by auxin, cytokinin, wounding and drought.
32880001	7	22	theme	cellulose	1108:1116	arg1	content					1118:1124	reduced cellulose content	1100:1124	reduced cellulose content	1100:1124	The stem of the transgenic plants showed longer xylem fibers and reduced cellulose content, while the leaf xylem had enhanced secondary cell wall thickness.
32880001	7	23	theme	xylem	1083:1087	arg1	fibers					1089:1094	longer xylem fibers	1076:1094	longer xylem fibers	1076:1094	The stem of the transgenic plants showed longer xylem fibers and reduced cellulose content, while the leaf xylem had enhanced secondary cell wall thickness.
32880001	3	24	theme	contrasting	526:536	arg1	phenotypes					538:547	contrasting phenotypes	526:547	contrasting phenotypes for wood quality and growth traits	526:582	Here, we report the functional characterization of a PHOSPHATE-INDUCED PROTEIN 1 homologue showing differential expression in xylem cells from Eucalyptus species of contrasting phenotypes for wood quality and growth traits.
32880001	9	25	theme	biotechnological	1583:1598	arg1	applications					1600:1611	biotechnological applications	1583:1611	biotechnological applications in forestry and biofuel crops	1583:1641	Taken together, the results suggest that EgPHI-1 mediates the elongation of secondary xylem fibers, secondary cell wall thickening and composition, and lignification, making it an attractive target for biotechnological applications in forestry and biofuel crops.
32880001	3	26	theme	PHOSPHATE-INDUCED	414:430	arg1	homologue					442:450	a PHOSPHATE-INDUCED PROTEIN 1 homologue	412:450	a PHOSPHATE-INDUCED PROTEIN 1 homologue	412:450	Here, we report the functional characterization of a PHOSPHATE-INDUCED PROTEIN 1 homologue showing differential expression in xylem cells from Eucalyptus species of contrasting phenotypes for wood quality and growth traits.
32880001	7	27	theme	wall	1176:1179	arg1	thickness					1181:1189	enhanced secondary cell wall thickness	1152:1189	enhanced secondary cell wall thickness	1152:1189	The stem of the transgenic plants showed longer xylem fibers and reduced cellulose content, while the leaf xylem had enhanced secondary cell wall thickness.
32880001	5	28	dep	Eucalyptus	786:795	arg1	globulus					797:804	globulus	797:804	globulus	797:804	Analysis of the promoter cis-acting regulatory elements and expression responses to different treatments revealed that the Eucalyptus globulus PHI-1 (EgPHI-1) is transcriptionally regulated by auxin, cytokinin, wounding and drought.
32880001	3	29	from	expression	473:482	arg1	cells					493:497	xylem cells	487:497	xylem cells from Eucalyptus species of contrasting phenotypes for wood quality and growth traits	487:582	Here, we report the functional characterization of a PHOSPHATE-INDUCED PROTEIN 1 homologue showing differential expression in xylem cells from Eucalyptus species of contrasting phenotypes for wood quality and growth traits.
32880001	3	29	from	expression	473:482	arg1	species					515:521	Eucalyptus species	504:521	Eucalyptus species of contrasting phenotypes for wood quality and growth traits	504:582	Here, we report the functional characterization of a PHOSPHATE-INDUCED PROTEIN 1 homologue showing differential expression in xylem cells from Eucalyptus species of contrasting phenotypes for wood quality and growth traits.
32880001	9	30	theme	attractive	1561:1570	arg1	target					1572:1577	an attractive target	1558:1577	it an attractive target for biotechnological applications in forestry and biofuel crops	1555:1641	Taken together, the results suggest that EgPHI-1 mediates the elongation of secondary xylem fibers, secondary cell wall thickening and composition, and lignification, making it an attractive target for biotechnological applications in forestry and biofuel crops.
32880001	2	31	from	overexpression	203:216	arg1	tobacco					221:227	tobacco	221:227	tobacco	221:227	Its overexpression in tobacco resulted in changes in biomass partitioning, xylem fiber length, secondary cell wall thickening and composition, and lignification.
32880001	8	32	theme	vessels	1263:1269	arg1	layers					1242:1247	individual cell wall layers	1221:1247	individual cell wall layers of fibers and vessels	1221:1269	UV microspectrophotometry of individual cell wall layers of fibers and vessels has shown that the transgenic plants exhibit differences in the lignification of S2 layer in both cell types.
32880001	5	33	theme	elements	710:717	arg1	Analysis					663:670	Analysis	663:670	Analysis of the promoter cis-acting regulatory elements	663:717	Analysis of the promoter cis-acting regulatory elements and expression responses to different treatments revealed that the Eucalyptus globulus PHI-1 (EgPHI-1) is transcriptionally regulated by auxin, cytokinin, wounding and drought.
32880001	5	33	theme	elements	710:717	arg1	responses					734:742	expression responses	723:742	expression responses to different treatments	723:766	Analysis of the promoter cis-acting regulatory elements and expression responses to different treatments revealed that the Eucalyptus globulus PHI-1 (EgPHI-1) is transcriptionally regulated by auxin, cytokinin, wounding and drought.
32880001	8	34	theme	cell	1232:1235	arg1	layers					1242:1247	individual cell wall layers	1221:1247	individual cell wall layers of fibers and vessels	1221:1269	UV microspectrophotometry of individual cell wall layers of fibers and vessels has shown that the transgenic plants exhibit differences in the lignification of S2 layer in both cell types.
32880001	3	35	theme	growth	570:575	arg1	traits					577:582	growth traits	570:582	growth traits	570:582	Here, we report the functional characterization of a PHOSPHATE-INDUCED PROTEIN 1 homologue showing differential expression in xylem cells from Eucalyptus species of contrasting phenotypes for wood quality and growth traits.
32880001	4	36	theme	family	655:660	arg1	member					627:632	a member	625:632	a member of the PHI-1/EXO/EXL family	625:660	Our results indicated that this gene is a member of the PHI-1/EXO/EXL family.
32880001	4	36	theme	family	655:660	arg1	gene					617:620	this gene	612:620	this gene	612:620	Our results indicated that this gene is a member of the PHI-1/EXO/EXL family.
32880001	0	37	theme	PHOSPHATE-INDUCED-1	11:29	arg1	EgPHI-1					0:6	EgPHI-1	0:6	EgPHI-1	0:6	EgPHI-1, a PHOSPHATE-INDUCED-1 gene from Eucalyptus globulus, is involved in shoot growth, xylem fiber length and secondary cell wall properties.
32880001	0	37	theme	PHOSPHATE-INDUCED-1	11:29	arg1	gene					31:34	a PHOSPHATE-INDUCED-1 gene	9:34	a PHOSPHATE-INDUCED-1 gene from Eucalyptus globulus	9:59	EgPHI-1, a PHOSPHATE-INDUCED-1 gene from Eucalyptus globulus, is involved in shoot growth, xylem fiber length and secondary cell wall properties.
32880001	5	38	theme	cis-acting	688:697	arg1	elements					710:717	the promoter cis-acting regulatory elements	675:717	the promoter cis-acting regulatory elements	675:717	Analysis of the promoter cis-acting regulatory elements and expression responses to different treatments revealed that the Eucalyptus globulus PHI-1 (EgPHI-1) is transcriptionally regulated by auxin, cytokinin, wounding and drought.
32880001	7	39	contain	had	1148:1150	arg2	thickness					1181:1189	enhanced secondary cell wall thickness	1152:1189	enhanced secondary cell wall thickness	1152:1189	The stem of the transgenic plants showed longer xylem fibers and reduced cellulose content, while the leaf xylem had enhanced secondary cell wall thickness.
32880001	7	39	contain	had	1148:1150	arg1	xylem					1142:1146	the leaf xylem	1133:1146	the leaf xylem	1133:1146	The stem of the transgenic plants showed longer xylem fibers and reduced cellulose content, while the leaf xylem had enhanced secondary cell wall thickness.
32880001	2	40	theme	fiber	280:284	arg1	length					286:291	xylem fiber length	274:291	xylem fiber length	274:291	Its overexpression in tobacco resulted in changes in biomass partitioning, xylem fiber length, secondary cell wall thickening and composition, and lignification.
32880001	3	41	theme	homologue	442:450	arg1	characterization					392:407	the functional characterization	377:407	the functional characterization of a PHOSPHATE-INDUCED PROTEIN 1 homologue	377:450	Here, we report the functional characterization of a PHOSPHATE-INDUCED PROTEIN 1 homologue showing differential expression in xylem cells from Eucalyptus species of contrasting phenotypes for wood quality and growth traits.
32880001	7	42	theme	cell	1171:1174	arg1	thickness					1181:1189	enhanced secondary cell wall thickness	1152:1189	enhanced secondary cell wall thickness	1152:1189	The stem of the transgenic plants showed longer xylem fibers and reduced cellulose content, while the leaf xylem had enhanced secondary cell wall thickness.
32880001	7	43	theme	plants	1062:1067	arg1	stem					1039:1042	The stem	1035:1042	The stem of the transgenic plants	1035:1067	The stem of the transgenic plants showed longer xylem fibers and reduced cellulose content, while the leaf xylem had enhanced secondary cell wall thickness.
32880001	0	44	theme	fiber	97:101	arg1	length					103:108	xylem fiber length	91:108	xylem fiber length	91:108	EgPHI-1, a PHOSPHATE-INDUCED-1 gene from Eucalyptus globulus, is involved in shoot growth, xylem fiber length and secondary cell wall properties.
32880001	2	45	theme	xylem	274:278	arg1	length					286:291	xylem fiber length	274:291	xylem fiber length	274:291	Its overexpression in tobacco resulted in changes in biomass partitioning, xylem fiber length, secondary cell wall thickening and composition, and lignification.
32880001	2	46	from	changes	241:247	arg1	partitioning					260:271	biomass partitioning	252:271	biomass partitioning	252:271	Its overexpression in tobacco resulted in changes in biomass partitioning, xylem fiber length, secondary cell wall thickening and composition, and lignification.
32880001	2	46	from	changes	241:247	arg1	lignification					346:358	lignification	346:358	lignification	346:358	Its overexpression in tobacco resulted in changes in biomass partitioning, xylem fiber length, secondary cell wall thickening and composition, and lignification.
32880001	2	46	from	changes	241:247	arg1	thickening					314:323	secondary cell wall thickening	294:323	secondary cell wall thickening	294:323	Its overexpression in tobacco resulted in changes in biomass partitioning, xylem fiber length, secondary cell wall thickening and composition, and lignification.
32880001	2	46	from	changes	241:247	arg1	length					286:291	xylem fiber length	274:291	xylem fiber length	274:291	Its overexpression in tobacco resulted in changes in biomass partitioning, xylem fiber length, secondary cell wall thickening and composition, and lignification.
32880001	1	47	theme	PHI-1/EXO/EXL	169:181	arg1	family					191:196	PHI-1/EXO/EXL protein family	169:196	PHI-1/EXO/EXL protein family	169:196	EgPHI-1 is a member of PHI-1/EXO/EXL protein family.
32880001	5	48	theme	Eucalyptus	786:795	arg1	PHI-1					806:810	the Eucalyptus globulus PHI-1	782:810	the Eucalyptus globulus PHI-1 (EgPHI-1)	782:820	Analysis of the promoter cis-acting regulatory elements and expression responses to different treatments revealed that the Eucalyptus globulus PHI-1 (EgPHI-1) is transcriptionally regulated by auxin, cytokinin, wounding and drought.
32880001	5	48	theme	Eucalyptus	786:795	arg1	EgPHI-1					813:819	EgPHI-1	813:819	EgPHI-1	813:819	Analysis of the promoter cis-acting regulatory elements and expression responses to different treatments revealed that the Eucalyptus globulus PHI-1 (EgPHI-1) is transcriptionally regulated by auxin, cytokinin, wounding and drought.
32880001	3	49	theme	xylem	487:491	arg1	cells					493:497	xylem cells	487:497	xylem cells from Eucalyptus species of contrasting phenotypes for wood quality and growth traits	487:582	Here, we report the functional characterization of a PHOSPHATE-INDUCED PROTEIN 1 homologue showing differential expression in xylem cells from Eucalyptus species of contrasting phenotypes for wood quality and growth traits.
32880001	0	50	theme	secondary	114:122	arg1	properties					134:143	secondary cell wall properties	114:143	secondary cell wall properties	114:143	EgPHI-1, a PHOSPHATE-INDUCED-1 gene from Eucalyptus globulus, is involved in shoot growth, xylem fiber length and secondary cell wall properties.
32880001	3	51	theme	PROTEIN	432:438	arg1	homologue					442:450	a PHOSPHATE-INDUCED PROTEIN 1 homologue	412:450	a PHOSPHATE-INDUCED PROTEIN 1 homologue	412:450	Here, we report the functional characterization of a PHOSPHATE-INDUCED PROTEIN 1 homologue showing differential expression in xylem cells from Eucalyptus species of contrasting phenotypes for wood quality and growth traits.
32880001	9	52	theme	secondary	1457:1465	arg1	fibers					1473:1478	secondary xylem fibers	1457:1478	secondary xylem fibers	1457:1478	Taken together, the results suggest that EgPHI-1 mediates the elongation of secondary xylem fibers, secondary cell wall thickening and composition, and lignification, making it an attractive target for biotechnological applications in forestry and biofuel crops.
32880001	1	53	theme	protein	183:189	arg1	family					191:196	PHI-1/EXO/EXL protein family	169:196	PHI-1/EXO/EXL protein family	169:196	EgPHI-1 is a member of PHI-1/EXO/EXL protein family.
32880001	3	54	theme	functional	381:390	arg1	characterization					392:407	the functional characterization	377:407	the functional characterization of a PHOSPHATE-INDUCED PROTEIN 1 homologue	377:450	Here, we report the functional characterization of a PHOSPHATE-INDUCED PROTEIN 1 homologue showing differential expression in xylem cells from Eucalyptus species of contrasting phenotypes for wood quality and growth traits.
32880001	6	55	theme	biomass	969:975	arg1	partitioning					953:964	the partitioning	949:964	the partitioning of biomass	949:975	EgPHI-1 overexpression in transgenic tobacco changed the partitioning of biomass, favoring its allocation to shoots in detriment of roots.
32880001	7	56	theme	enhanced	1152:1159	arg1	thickness					1181:1189	enhanced secondary cell wall thickness	1152:1189	enhanced secondary cell wall thickness	1152:1189	The stem of the transgenic plants showed longer xylem fibers and reduced cellulose content, while the leaf xylem had enhanced secondary cell wall thickness.
32880001	7	57	theme	secondary	1161:1169	arg1	thickness					1181:1189	enhanced secondary cell wall thickness	1152:1189	enhanced secondary cell wall thickness	1152:1189	The stem of the transgenic plants showed longer xylem fibers and reduced cellulose content, while the leaf xylem had enhanced secondary cell wall thickness.
32880001	1	58	theme	family	191:196	arg1	EgPHI-1					146:152	EgPHI-1	146:152	EgPHI-1	146:152	EgPHI-1 is a member of PHI-1/EXO/EXL protein family.
32880001	1	58	theme	family	191:196	arg1	member					159:164	a member	157:164	a member of PHI-1/EXO/EXL protein family	157:196	EgPHI-1 is a member of PHI-1/EXO/EXL protein family.
32880001	7	59	theme	leaf	1137:1140	arg1	xylem					1142:1146	the leaf xylem	1133:1146	the leaf xylem	1133:1146	The stem of the transgenic plants showed longer xylem fibers and reduced cellulose content, while the leaf xylem had enhanced secondary cell wall thickness.
32880001	0	60	theme	wall	129:132	arg1	properties					134:143	secondary cell wall properties	114:143	secondary cell wall properties	114:143	EgPHI-1, a PHOSPHATE-INDUCED-1 gene from Eucalyptus globulus, is involved in shoot growth, xylem fiber length and secondary cell wall properties.
32880001	9	61	theme	wall	1496:1499	arg1	thickening					1501:1510	secondary cell wall thickening	1481:1510	secondary cell wall thickening	1481:1510	Taken together, the results suggest that EgPHI-1 mediates the elongation of secondary xylem fibers, secondary cell wall thickening and composition, and lignification, making it an attractive target for biotechnological applications in forestry and biofuel crops.
32880001	9	62	theme	forestry	1616:1623	arg1	crops					1637:1641	forestry and biofuel crops	1616:1641	forestry and biofuel crops	1616:1641	Taken together, the results suggest that EgPHI-1 mediates the elongation of secondary xylem fibers, secondary cell wall thickening and composition, and lignification, making it an attractive target for biotechnological applications in forestry and biofuel crops.
32880001	8	63	theme	cell	1369:1372	arg1	types					1374:1378	both cell types	1364:1378	both cell types	1364:1378	UV microspectrophotometry of individual cell wall layers of fibers and vessels has shown that the transgenic plants exhibit differences in the lignification of S2 layer in both cell types.
32880001	9	64	theme	cell	1491:1494	arg1	thickening					1501:1510	secondary cell wall thickening	1481:1510	secondary cell wall thickening	1481:1510	Taken together, the results suggest that EgPHI-1 mediates the elongation of secondary xylem fibers, secondary cell wall thickening and composition, and lignification, making it an attractive target for biotechnological applications in forestry and biofuel crops.
32880001	8	65	theme	wall	1237:1240	arg1	layers					1242:1247	individual cell wall layers	1221:1247	individual cell wall layers of fibers and vessels	1221:1269	UV microspectrophotometry of individual cell wall layers of fibers and vessels has shown that the transgenic plants exhibit differences in the lignification of S2 layer in both cell types.
32880001	0	66	theme	cell	124:127	arg1	properties					134:143	secondary cell wall properties	114:143	secondary cell wall properties	114:143	EgPHI-1, a PHOSPHATE-INDUCED-1 gene from Eucalyptus globulus, is involved in shoot growth, xylem fiber length and secondary cell wall properties.
32880001	8	67	theme	individual	1221:1230	arg1	layers					1242:1247	individual cell wall layers	1221:1247	individual cell wall layers of fibers and vessels	1221:1269	UV microspectrophotometry of individual cell wall layers of fibers and vessels has shown that the transgenic plants exhibit differences in the lignification of S2 layer in both cell types.
32880001	2	68	theme	wall	309:312	arg1	thickening					314:323	secondary cell wall thickening	294:323	secondary cell wall thickening	294:323	Its overexpression in tobacco resulted in changes in biomass partitioning, xylem fiber length, secondary cell wall thickening and composition, and lignification.
32880001	9	69	theme	biofuel	1629:1635	arg1	crops					1637:1641	forestry and biofuel crops	1616:1641	forestry and biofuel crops	1616:1641	Taken together, the results suggest that EgPHI-1 mediates the elongation of secondary xylem fibers, secondary cell wall thickening and composition, and lignification, making it an attractive target for biotechnological applications in forestry and biofuel crops.
32880001	6	70	from	overexpression	904:917	arg1	tobacco					933:939	transgenic tobacco	922:939	transgenic tobacco	922:939	EgPHI-1 overexpression in transgenic tobacco changed the partitioning of biomass, favoring its allocation to shoots in detriment of roots.
32880001	2	71	theme	cell	304:307	arg1	thickening					314:323	secondary cell wall thickening	294:323	secondary cell wall thickening	294:323	Its overexpression in tobacco resulted in changes in biomass partitioning, xylem fiber length, secondary cell wall thickening and composition, and lignification.
32880001	3	72	theme	wood	553:556	arg1	quality					558:564	wood quality	553:564	wood quality	553:564	Here, we report the functional characterization of a PHOSPHATE-INDUCED PROTEIN 1 homologue showing differential expression in xylem cells from Eucalyptus species of contrasting phenotypes for wood quality and growth traits.
32880001	5	73	theme	promoter	679:686	arg1	elements					710:717	the promoter cis-acting regulatory elements	675:717	the promoter cis-acting regulatory elements	675:717	Analysis of the promoter cis-acting regulatory elements and expression responses to different treatments revealed that the Eucalyptus globulus PHI-1 (EgPHI-1) is transcriptionally regulated by auxin, cytokinin, wounding and drought.
32880001	8	74	theme	layer	1355:1359	arg1	lignification					1335:1347	the lignification	1331:1347	the lignification of S2 layer in both cell types	1331:1378	UV microspectrophotometry of individual cell wall layers of fibers and vessels has shown that the transgenic plants exhibit differences in the lignification of S2 layer in both cell types.
32880001	2	75	theme	secondary	294:302	arg1	thickening					314:323	secondary cell wall thickening	294:323	secondary cell wall thickening	294:323	Its overexpression in tobacco resulted in changes in biomass partitioning, xylem fiber length, secondary cell wall thickening and composition, and lignification.
32880001	3	76	theme	differential	460:471	arg1	expression					473:482	differential expression	460:482	differential expression in xylem cells from Eucalyptus species of contrasting phenotypes for wood quality and growth traits	460:582	Here, we report the functional characterization of a PHOSPHATE-INDUCED PROTEIN 1 homologue showing differential expression in xylem cells from Eucalyptus species of contrasting phenotypes for wood quality and growth traits.
32880001	9	77	theme	secondary	1481:1489	arg1	thickening					1501:1510	secondary cell wall thickening	1481:1510	secondary cell wall thickening	1481:1510	Taken together, the results suggest that EgPHI-1 mediates the elongation of secondary xylem fibers, secondary cell wall thickening and composition, and lignification, making it an attractive target for biotechnological applications in forestry and biofuel crops.
32880001	9	78	from	applications	1600:1611	arg1	crops					1637:1641	forestry and biofuel crops	1616:1641	forestry and biofuel crops	1616:1641	Taken together, the results suggest that EgPHI-1 mediates the elongation of secondary xylem fibers, secondary cell wall thickening and composition, and lignification, making it an attractive target for biotechnological applications in forestry and biofuel crops.
32880001	9	79	theme	xylem	1467:1471	arg1	fibers					1473:1478	secondary xylem fibers	1457:1478	secondary xylem fibers	1457:1478	Taken together, the results suggest that EgPHI-1 mediates the elongation of secondary xylem fibers, secondary cell wall thickening and composition, and lignification, making it an attractive target for biotechnological applications in forestry and biofuel crops.
32880001	3	80	theme	Eucalyptus	504:513	arg1	species					515:521	Eucalyptus species	504:521	Eucalyptus species of contrasting phenotypes for wood quality and growth traits	504:582	Here, we report the functional characterization of a PHOSPHATE-INDUCED PROTEIN 1 homologue showing differential expression in xylem cells from Eucalyptus species of contrasting phenotypes for wood quality and growth traits.
32880001	6	81	theme	roots	1028:1032	arg1	detriment					1015:1023	detriment	1015:1023	detriment of roots	1015:1032	EgPHI-1 overexpression in transgenic tobacco changed the partitioning of biomass, favoring its allocation to shoots in detriment of roots.
32880001	8	82	theme	layers	1242:1247	arg1	microspectrophotometry					1195:1216	UV microspectrophotometry	1192:1216	UV microspectrophotometry of individual cell wall layers of fibers and vessels	1192:1269	UV microspectrophotometry of individual cell wall layers of fibers and vessels has shown that the transgenic plants exhibit differences in the lignification of S2 layer in both cell types.
32880001	8	83	theme	UV	1192:1193	arg1	microspectrophotometry					1195:1216	UV microspectrophotometry	1192:1216	UV microspectrophotometry of individual cell wall layers of fibers and vessels	1192:1269	UV microspectrophotometry of individual cell wall layers of fibers and vessels has shown that the transgenic plants exhibit differences in the lignification of S2 layer in both cell types.
32880001	8	84	theme	transgenic	1290:1299	arg1	plants					1301:1306	the transgenic plants	1286:1306	the transgenic plants	1286:1306	UV microspectrophotometry of individual cell wall layers of fibers and vessels has shown that the transgenic plants exhibit differences in the lignification of S2 layer in both cell types.
33744788	0	0	theme	oryzae	78:83	arg1	pathogenicity					49:61	the pathogenicity	45:61	the pathogenicity of Pyricularia oryzae	45:83	The chitin deacetylase PoCda7 is involved in the pathogenicity of Pyricularia oryzae.
33744788	5	1	theme	PoCda7	733:738	arg1	functions					720:728	the functions	716:728	the functions of PoCda7, a chitin deacetylase in Pyricularia oryzae	716:782	The present study revealed the functions of PoCda7, a chitin deacetylase in Pyricularia oryzae.
33744788	3	2	with	co-evolution	484:495	arg1	nature					502:507	nature	502:507	nature	502:507	Fungi developed infection-related changes in cell wall composition in co-evolution with nature to solve this problem.
33744788	11	3	from	effector	1731:1738	arg1	oryzae					1746:1751	P. oryzae	1743:1751	P. oryzae	1743:1751	Subcellular localization experiments showed that PoCda7 was localized in the cell wall, and its fluorescence transferred to the EIHM and BIC when the rice blast fungus infected the rice leaf sheath, which was referred to as a candidate apoplastic effector in P. oryzae.
33744788	8	4	theme	fungal	1260:1265	arg1	invasion					1246:1253	decreased appressorium-mediated invasion	1214:1253	decreased appressorium-mediated invasion	1214:1253	As a result of the decreased appressorium turgor pressure and decreased appressorium-mediated invasion, the fungal virulence of ΔPocda7 was significantly reduced in host plants.
33744788	8	4	theme	fungal	1260:1265	arg1	result					1157:1162	a result	1155:1162	a result of the decreased appressorium turgor pressure	1155:1208	As a result of the decreased appressorium turgor pressure and decreased appressorium-mediated invasion, the fungal virulence of ΔPocda7 was significantly reduced in host plants.
33744788	8	4	theme	fungal	1260:1265	arg1	virulence					1267:1275	the fungal virulence	1256:1275	the fungal virulence of ΔPocda7	1256:1286	As a result of the decreased appressorium turgor pressure and decreased appressorium-mediated invasion, the fungal virulence of ΔPocda7 was significantly reduced in host plants.
33744788	0	5	theme	Pyricularia	66:76	arg1	oryzae					78:83	Pyricularia oryzae	66:83	Pyricularia oryzae	66:83	The chitin deacetylase PoCda7 is involved in the pathogenicity of Pyricularia oryzae.
33744788	6	6	theme	fungal	878:883	arg1	development					897:907	fungal morphogenic development	878:907	fungal morphogenic development	878:907	Phenotype analysis revealed that the knockout mutant of ΔPocda7 had no significant effect on fungal morphogenic development, including conidiation, germination, appressorial formation and cell wall of conidium and hyphae but was sensitive to reactive oxygen species.
33744788	6	7	theme	Phenotype	785:793	arg1	analysis					795:802	Phenotype analysis	785:802	Phenotype analysis	785:802	Phenotype analysis revealed that the knockout mutant of ΔPocda7 had no significant effect on fungal morphogenic development, including conidiation, germination, appressorial formation and cell wall of conidium and hyphae but was sensitive to reactive oxygen species.
33744788	1	8	theme	cellular	146:153	arg1	integrity					155:163	cellular integrity	146:163	cellular integrity	146:163	The fungal cell wall plays an essential role in maintaining cellular integrity and facing complex and changing environmental conditions.
33744788	5	9	theme	present	693:699	arg1	study					701:705	The present study	689:705	The present study	689:705	The present study revealed the functions of PoCda7, a chitin deacetylase in Pyricularia oryzae.
33744788	5	10	dep	Pyricularia	765:775	arg1	oryzae					777:782	Pyricularia oryzae	765:782	Pyricularia oryzae	765:782	The present study revealed the functions of PoCda7, a chitin deacetylase in Pyricularia oryzae.
33744788	6	11	theme	cell	973:976	arg1	wall					978:981	cell wall	973:981	cell wall of conidium and hyphae	973:1004	Phenotype analysis revealed that the knockout mutant of ΔPocda7 had no significant effect on fungal morphogenic development, including conidiation, germination, appressorial formation and cell wall of conidium and hyphae but was sensitive to reactive oxygen species.
33744788	10	12	theme	PoCDA7	1430:1435	arg1	expression					1416:1425	the expression	1412:1425	the expression of PoCDA7	1412:1435	Additionally, the expression of PoCDA7 was repressed in the early stage of infection.
33744788	11	13	dep	infected	1652:1659	arg1	referred					1693:1700	referred	1693:1700	was referred to as a candidate apoplastic effector in P. oryzae	1689:1751	Subcellular localization experiments showed that PoCda7 was localized in the cell wall, and its fluorescence transferred to the EIHM and BIC when the rice blast fungus infected the rice leaf sheath, which was referred to as a candidate apoplastic effector in P. oryzae.
33744788	3	14	theme	wall	464:467	arg1	composition					469:479	cell wall composition	459:479	cell wall composition	459:479	Fungi developed infection-related changes in cell wall composition in co-evolution with nature to solve this problem.
33744788	11	15	theme	blast	1639:1643	arg1	fungus					1645:1650	the rice blast fungus	1630:1650	the rice blast fungus	1630:1650	Subcellular localization experiments showed that PoCda7 was localized in the cell wall, and its fluorescence transferred to the EIHM and BIC when the rice blast fungus infected the rice leaf sheath, which was referred to as a candidate apoplastic effector in P. oryzae.
33744788	6	16	theme	significant	856:866	arg1	effect					868:873	no significant effect	853:873	no significant effect	853:873	Phenotype analysis revealed that the knockout mutant of ΔPocda7 had no significant effect on fungal morphogenic development, including conidiation, germination, appressorial formation and cell wall of conidium and hyphae but was sensitive to reactive oxygen species.
33744788	2	17	theme	fungal	380:385	arg1	wall					392:395	the fungal cell wall	376:395	the fungal cell wall	376:395	Whether a fungus successfully invades a host depends on whether it evades the plant's innate immune system, which recognizes the conserved components of the fungal cell wall, such as chitin.
33744788	8	18	theme	pressure	1201:1208	arg1	invasion					1246:1253	decreased appressorium-mediated invasion	1214:1253	decreased appressorium-mediated invasion	1214:1253	As a result of the decreased appressorium turgor pressure and decreased appressorium-mediated invasion, the fungal virulence of ΔPocda7 was significantly reduced in host plants.
33744788	8	18	theme	pressure	1201:1208	arg1	result					1157:1162	a result	1155:1162	a result of the decreased appressorium turgor pressure	1155:1208	As a result of the decreased appressorium turgor pressure and decreased appressorium-mediated invasion, the fungal virulence of ΔPocda7 was significantly reduced in host plants.
33744788	8	18	theme	pressure	1201:1208	arg1	virulence					1267:1275	the fungal virulence	1256:1275	the fungal virulence of ΔPocda7	1256:1286	As a result of the decreased appressorium turgor pressure and decreased appressorium-mediated invasion, the fungal virulence of ΔPocda7 was significantly reduced in host plants.
33744788	11	19	theme	candidate	1710:1718	arg1	effector					1731:1738	a candidate apoplastic effector	1708:1738	a candidate apoplastic effector in P. oryzae	1708:1751	Subcellular localization experiments showed that PoCda7 was localized in the cell wall, and its fluorescence transferred to the EIHM and BIC when the rice blast fungus infected the rice leaf sheath, which was referred to as a candidate apoplastic effector in P. oryzae.
33744788	11	19	theme	candidate	1710:1718	arg1	which					1683:1687	which	1683:1687	which	1683:1687	Subcellular localization experiments showed that PoCda7 was localized in the cell wall, and its fluorescence transferred to the EIHM and BIC when the rice blast fungus infected the rice leaf sheath, which was referred to as a candidate apoplastic effector in P. oryzae.
33744788	10	20	theme	infection	1473:1481	arg1	stage					1464:1468	the early stage	1454:1468	the early stage of infection	1454:1481	Additionally, the expression of PoCDA7 was repressed in the early stage of infection.
33744788	2	21	theme	innate	309:314	arg1	system					323:328	the plant's innate immune system	297:328	the plant's innate immune system	297:328	Whether a fungus successfully invades a host depends on whether it evades the plant's innate immune system, which recognizes the conserved components of the fungal cell wall, such as chitin.
33744788	8	22	theme	decreased	1214:1222	arg1	invasion					1246:1253	decreased appressorium-mediated invasion	1214:1253	decreased appressorium-mediated invasion	1214:1253	As a result of the decreased appressorium turgor pressure and decreased appressorium-mediated invasion, the fungal virulence of ΔPocda7 was significantly reduced in host plants.
33744788	8	22	theme	decreased	1214:1222	arg1	virulence					1267:1275	the fungal virulence	1256:1275	the fungal virulence of ΔPocda7	1256:1286	As a result of the decreased appressorium turgor pressure and decreased appressorium-mediated invasion, the fungal virulence of ΔPocda7 was significantly reduced in host plants.
33744788	0	23	theme	chitin	4:9	arg1	PoCda7					23:28	The chitin deacetylase PoCda7	0:28	The chitin deacetylase PoCda7	0:28	The chitin deacetylase PoCda7 is involved in the pathogenicity of Pyricularia oryzae.
33744788	9	24	theme	cell	1351:1354	arg1	death					1356:1360	the cell death	1347:1360	the cell death of leaves in Nicotiana benthamiana	1347:1395	PoCda7 inhibited the cell death of leaves in Nicotiana benthamiana.
33744788	10	25	theme	early	1458:1462	arg1	stage					1464:1468	the early stage	1454:1468	the early stage of infection	1454:1481	Additionally, the expression of PoCDA7 was repressed in the early stage of infection.
33744788	11	26	theme	rice	1665:1668	arg1	sheath					1675:1680	the rice leaf sheath	1661:1680	the rice leaf sheath	1661:1680	Subcellular localization experiments showed that PoCda7 was localized in the cell wall, and its fluorescence transferred to the EIHM and BIC when the rice blast fungus infected the rice leaf sheath, which was referred to as a candidate apoplastic effector in P. oryzae.
33744788	1	27	theme	facing	169:174	arg1	complex					176:182	facing complex	169:182	facing complex	169:182	The fungal cell wall plays an essential role in maintaining cellular integrity and facing complex and changing environmental conditions.
33744788	8	28	theme	appressorium-mediated	1224:1244	arg1	invasion					1246:1253	decreased appressorium-mediated invasion	1214:1253	decreased appressorium-mediated invasion	1214:1253	As a result of the decreased appressorium turgor pressure and decreased appressorium-mediated invasion, the fungal virulence of ΔPocda7 was significantly reduced in host plants.
33744788	8	28	theme	appressorium-mediated	1224:1244	arg1	virulence					1267:1275	the fungal virulence	1256:1275	the fungal virulence of ΔPocda7	1256:1286	As a result of the decreased appressorium turgor pressure and decreased appressorium-mediated invasion, the fungal virulence of ΔPocda7 was significantly reduced in host plants.
33744788	11	29	theme	cell	1561:1564	arg1	wall					1566:1569	the cell wall	1557:1569	the cell wall	1557:1569	Subcellular localization experiments showed that PoCda7 was localized in the cell wall, and its fluorescence transferred to the EIHM and BIC when the rice blast fungus infected the rice leaf sheath, which was referred to as a candidate apoplastic effector in P. oryzae.
33744788	6	30	theme	oxygen	1036:1041	arg1	species					1043:1049	reactive oxygen species	1027:1049	reactive oxygen species	1027:1049	Phenotype analysis revealed that the knockout mutant of ΔPocda7 had no significant effect on fungal morphogenic development, including conidiation, germination, appressorial formation and cell wall of conidium and hyphae but was sensitive to reactive oxygen species.
33744788	8	31	theme	decreased	1171:1179	arg1	pressure					1201:1208	the decreased appressorium turgor pressure	1167:1208	the decreased appressorium turgor pressure	1167:1208	As a result of the decreased appressorium turgor pressure and decreased appressorium-mediated invasion, the fungal virulence of ΔPocda7 was significantly reduced in host plants.
33744788	6	32	theme	appressorial	946:957	arg1	formation					959:967	appressorial formation	946:967	appressorial formation	946:967	Phenotype analysis revealed that the knockout mutant of ΔPocda7 had no significant effect on fungal morphogenic development, including conidiation, germination, appressorial formation and cell wall of conidium and hyphae but was sensitive to reactive oxygen species.
33744788	1	33	theme	fungal	90:95	arg1	wall					102:105	The fungal cell wall	86:105	The fungal cell wall	86:105	The fungal cell wall plays an essential role in maintaining cellular integrity and facing complex and changing environmental conditions.
33744788	11	34	dep	EIHM	1612:1615	arg1	the					1608:1610	the	1608:1610	the	1608:1610	Subcellular localization experiments showed that PoCda7 was localized in the cell wall, and its fluorescence transferred to the EIHM and BIC when the rice blast fungus infected the rice leaf sheath, which was referred to as a candidate apoplastic effector in P. oryzae.
33744788	6	35	contain	had	849:851	arg1	mutant					831:836	the knockout mutant	818:836	the knockout mutant of ΔPocda7	818:847	Phenotype analysis revealed that the knockout mutant of ΔPocda7 had no significant effect on fungal morphogenic development, including conidiation, germination, appressorial formation and cell wall of conidium and hyphae but was sensitive to reactive oxygen species.
33744788	6	35	contain	had	849:851	arg2	effect					868:873	no significant effect	853:873	no significant effect	853:873	Phenotype analysis revealed that the knockout mutant of ΔPocda7 had no significant effect on fungal morphogenic development, including conidiation, germination, appressorial formation and cell wall of conidium and hyphae but was sensitive to reactive oxygen species.
33744788	6	35	contain	had	849:851	arg1	sensitive					1014:1022	sensitive	1014:1022	sensitive	1014:1022	Phenotype analysis revealed that the knockout mutant of ΔPocda7 had no significant effect on fungal morphogenic development, including conidiation, germination, appressorial formation and cell wall of conidium and hyphae but was sensitive to reactive oxygen species.
33744788	0	36	theme	deacetylase	11:21	arg1	PoCda7					23:28	The chitin deacetylase PoCda7	0:28	The chitin deacetylase PoCda7	0:28	The chitin deacetylase PoCda7 is involved in the pathogenicity of Pyricularia oryzae.
33744788	4	37	theme	changes	543:549	arg1	deacetylation					558:570	the deacetylation	554:570	the deacetylation of chitin by chitin deacetylase (CDA)	554:608	One of the changes is the deacetylation of chitin by chitin deacetylase (CDA) to produce a polysaccharide that influences the infection of pathogenic fungi.
33744788	4	37	theme	changes	543:549	arg1	One					532:534	One	532:534	One	532:534	One of the changes is the deacetylation of chitin by chitin deacetylase (CDA) to produce a polysaccharide that influences the infection of pathogenic fungi.
33744788	4	37	theme	changes	543:549	arg1	changes					543:549	the changes	539:549	the changes	539:549	One of the changes is the deacetylation of chitin by chitin deacetylase (CDA) to produce a polysaccharide that influences the infection of pathogenic fungi.
33744788	6	38	theme	ΔPocda7	841:847	arg1	mutant					831:836	the knockout mutant	818:836	the knockout mutant of ΔPocda7	818:847	Phenotype analysis revealed that the knockout mutant of ΔPocda7 had no significant effect on fungal morphogenic development, including conidiation, germination, appressorial formation and cell wall of conidium and hyphae but was sensitive to reactive oxygen species.
33744788	6	38	theme	ΔPocda7	841:847	arg1	sensitive					1014:1022	sensitive	1014:1022	sensitive	1014:1022	Phenotype analysis revealed that the knockout mutant of ΔPocda7 had no significant effect on fungal morphogenic development, including conidiation, germination, appressorial formation and cell wall of conidium and hyphae but was sensitive to reactive oxygen species.
33744788	1	39	theme	cell	97:100	arg1	wall					102:105	The fungal cell wall	86:105	The fungal cell wall	86:105	The fungal cell wall plays an essential role in maintaining cellular integrity and facing complex and changing environmental conditions.
33744788	6	40	theme	morphogenic	885:895	arg1	development					897:907	fungal morphogenic development	878:907	fungal morphogenic development	878:907	Phenotype analysis revealed that the knockout mutant of ΔPocda7 had no significant effect on fungal morphogenic development, including conidiation, germination, appressorial formation and cell wall of conidium and hyphae but was sensitive to reactive oxygen species.
33744788	8	41	theme	host	1317:1320	arg1	plants					1322:1327	host plants	1317:1327	host plants	1317:1327	As a result of the decreased appressorium turgor pressure and decreased appressorium-mediated invasion, the fungal virulence of ΔPocda7 was significantly reduced in host plants.
33744788	8	42	theme	turgor	1194:1199	arg1	pressure					1201:1208	the decreased appressorium turgor pressure	1167:1208	the decreased appressorium turgor pressure	1167:1208	As a result of the decreased appressorium turgor pressure and decreased appressorium-mediated invasion, the fungal virulence of ΔPocda7 was significantly reduced in host plants.
33744788	8	43	theme	ΔPocda7	1280:1286	arg1	invasion					1246:1253	decreased appressorium-mediated invasion	1214:1253	decreased appressorium-mediated invasion	1214:1253	As a result of the decreased appressorium turgor pressure and decreased appressorium-mediated invasion, the fungal virulence of ΔPocda7 was significantly reduced in host plants.
33744788	8	43	theme	ΔPocda7	1280:1286	arg1	result					1157:1162	a result	1155:1162	a result of the decreased appressorium turgor pressure	1155:1208	As a result of the decreased appressorium turgor pressure and decreased appressorium-mediated invasion, the fungal virulence of ΔPocda7 was significantly reduced in host plants.
33744788	8	43	theme	ΔPocda7	1280:1286	arg1	virulence					1267:1275	the fungal virulence	1256:1275	the fungal virulence of ΔPocda7	1256:1286	As a result of the decreased appressorium turgor pressure and decreased appressorium-mediated invasion, the fungal virulence of ΔPocda7 was significantly reduced in host plants.
33744788	1	44	theme	environmental	197:209	arg1	conditions					211:220	environmental conditions	197:220	environmental conditions	197:220	The fungal cell wall plays an essential role in maintaining cellular integrity and facing complex and changing environmental conditions.
33744788	2	45	theme	wall	392:395	arg1	components					362:371	the conserved components	348:371	the conserved components	348:371	Whether a fungus successfully invades a host depends on whether it evades the plant's innate immune system, which recognizes the conserved components of the fungal cell wall, such as chitin.
33744788	2	45	theme	wall	392:395	arg1	chitin					406:411	chitin	406:411	chitin	406:411	Whether a fungus successfully invades a host depends on whether it evades the plant's innate immune system, which recognizes the conserved components of the fungal cell wall, such as chitin.
33744788	5	46	theme	chitin	743:748	arg1	deacetylase					750:760	a chitin deacetylase	741:760	a chitin deacetylase in Pyricularia oryzae	741:782	The present study revealed the functions of PoCda7, a chitin deacetylase in Pyricularia oryzae.
33744788	5	46	theme	chitin	743:748	arg1	PoCda7					733:738	PoCda7	733:738	PoCda7	733:738	The present study revealed the functions of PoCda7, a chitin deacetylase in Pyricularia oryzae.
33744788	7	47	theme	host	1138:1141	arg1	surface					1143:1149	the host surface	1134:1149	the host surface	1134:1149	Glycerols are necessary to generate sufficient turgor in appressoria for invading the host surface.
33744788	11	48	theme	leaf	1670:1673	arg1	sheath					1675:1680	the rice leaf sheath	1661:1680	the rice leaf sheath	1661:1680	Subcellular localization experiments showed that PoCda7 was localized in the cell wall, and its fluorescence transferred to the EIHM and BIC when the rice blast fungus infected the rice leaf sheath, which was referred to as a candidate apoplastic effector in P. oryzae.
33744788	3	49	theme	infection-related	430:446	arg1	changes					448:454	infection-related changes	430:454	infection-related changes in cell wall composition in co-evolution with nature	430:507	Fungi developed infection-related changes in cell wall composition in co-evolution with nature to solve this problem.
33744788	7	50	from	turgor	1099:1104	arg1	appressoria					1109:1119	appressoria	1109:1119	appressoria	1109:1119	Glycerols are necessary to generate sufficient turgor in appressoria for invading the host surface.
33744788	8	51	theme	appressorium	1181:1192	arg1	pressure					1201:1208	the decreased appressorium turgor pressure	1167:1208	the decreased appressorium turgor pressure	1167:1208	As a result of the decreased appressorium turgor pressure and decreased appressorium-mediated invasion, the fungal virulence of ΔPocda7 was significantly reduced in host plants.
33744788	6	52	theme	knockout	822:829	arg1	mutant					831:836	the knockout mutant	818:836	the knockout mutant of ΔPocda7	818:847	Phenotype analysis revealed that the knockout mutant of ΔPocda7 had no significant effect on fungal morphogenic development, including conidiation, germination, appressorial formation and cell wall of conidium and hyphae but was sensitive to reactive oxygen species.
33744788	6	52	theme	knockout	822:829	arg1	sensitive					1014:1022	sensitive	1014:1022	sensitive	1014:1022	Phenotype analysis revealed that the knockout mutant of ΔPocda7 had no significant effect on fungal morphogenic development, including conidiation, germination, appressorial formation and cell wall of conidium and hyphae but was sensitive to reactive oxygen species.
33744788	4	53	theme	fungi	682:686	arg1	infection					658:666	the infection	654:666	the infection of pathogenic fungi	654:686	One of the changes is the deacetylation of chitin by chitin deacetylase (CDA) to produce a polysaccharide that influences the infection of pathogenic fungi.
33744788	4	54	theme	chitin	575:580	arg1	deacetylation					558:570	the deacetylation	554:570	the deacetylation of chitin by chitin deacetylase (CDA)	554:608	One of the changes is the deacetylation of chitin by chitin deacetylase (CDA) to produce a polysaccharide that influences the infection of pathogenic fungi.
33744788	4	54	theme	chitin	575:580	arg1	One					532:534	One	532:534	One	532:534	One of the changes is the deacetylation of chitin by chitin deacetylase (CDA) to produce a polysaccharide that influences the infection of pathogenic fungi.
33744788	4	54	theme	chitin	575:580	arg1	changes					543:549	the changes	539:549	the changes	539:549	One of the changes is the deacetylation of chitin by chitin deacetylase (CDA) to produce a polysaccharide that influences the infection of pathogenic fungi.
33744788	9	55	theme	leaves	1365:1370	arg1	death					1356:1360	the cell death	1347:1360	the cell death of leaves in Nicotiana benthamiana	1347:1395	PoCda7 inhibited the cell death of leaves in Nicotiana benthamiana.
33744788	11	56	theme	rice	1634:1637	arg1	fungus					1645:1650	the rice blast fungus	1630:1650	the rice blast fungus	1630:1650	Subcellular localization experiments showed that PoCda7 was localized in the cell wall, and its fluorescence transferred to the EIHM and BIC when the rice blast fungus infected the rice leaf sheath, which was referred to as a candidate apoplastic effector in P. oryzae.
33744788	3	57	from	changes	448:454	arg1	composition					469:479	cell wall composition	459:479	cell wall composition	459:479	Fungi developed infection-related changes in cell wall composition in co-evolution with nature to solve this problem.
33744788	3	57	from	changes	448:454	arg1	co-evolution					484:495	co-evolution	484:495	co-evolution with nature	484:507	Fungi developed infection-related changes in cell wall composition in co-evolution with nature to solve this problem.
33744788	4	58	theme	pathogenic	671:680	arg1	fungi					682:686	pathogenic fungi	671:686	pathogenic fungi	671:686	One of the changes is the deacetylation of chitin by chitin deacetylase (CDA) to produce a polysaccharide that influences the infection of pathogenic fungi.
33744788	11	59	theme	Subcellular	1484:1494	arg1	experiments					1509:1519	Subcellular localization experiments	1484:1519	Subcellular localization experiments	1484:1519	Subcellular localization experiments showed that PoCda7 was localized in the cell wall, and its fluorescence transferred to the EIHM and BIC when the rice blast fungus infected the rice leaf sheath, which was referred to as a candidate apoplastic effector in P. oryzae.
33744788	6	60	theme	hyphae	999:1004	arg1	conidiation					920:930	conidiation	920:930	conidiation	920:930	Phenotype analysis revealed that the knockout mutant of ΔPocda7 had no significant effect on fungal morphogenic development, including conidiation, germination, appressorial formation and cell wall of conidium and hyphae but was sensitive to reactive oxygen species.
33744788	6	60	theme	hyphae	999:1004	arg1	wall					978:981	cell wall	973:981	cell wall of conidium and hyphae	973:1004	Phenotype analysis revealed that the knockout mutant of ΔPocda7 had no significant effect on fungal morphogenic development, including conidiation, germination, appressorial formation and cell wall of conidium and hyphae but was sensitive to reactive oxygen species.
33744788	6	60	theme	hyphae	999:1004	arg1	germination					933:943	germination	933:943	germination	933:943	Phenotype analysis revealed that the knockout mutant of ΔPocda7 had no significant effect on fungal morphogenic development, including conidiation, germination, appressorial formation and cell wall of conidium and hyphae but was sensitive to reactive oxygen species.
33744788	6	60	theme	hyphae	999:1004	arg1	formation					959:967	appressorial formation	946:967	appressorial formation	946:967	Phenotype analysis revealed that the knockout mutant of ΔPocda7 had no significant effect on fungal morphogenic development, including conidiation, germination, appressorial formation and cell wall of conidium and hyphae but was sensitive to reactive oxygen species.
33744788	2	61	theme	immune	316:321	arg1	system					323:328	the plant's innate immune system	297:328	the plant's innate immune system	297:328	Whether a fungus successfully invades a host depends on whether it evades the plant's innate immune system, which recognizes the conserved components of the fungal cell wall, such as chitin.
33744788	11	62	theme	localization	1496:1507	arg1	experiments					1509:1519	Subcellular localization experiments	1484:1519	Subcellular localization experiments	1484:1519	Subcellular localization experiments showed that PoCda7 was localized in the cell wall, and its fluorescence transferred to the EIHM and BIC when the rice blast fungus infected the rice leaf sheath, which was referred to as a candidate apoplastic effector in P. oryzae.
33744788	9	63	theme	Nicotiana	1375:1383	arg1	benthamiana					1385:1395	Nicotiana benthamiana	1375:1395	Nicotiana benthamiana	1375:1395	PoCda7 inhibited the cell death of leaves in Nicotiana benthamiana.
33744788	11	64	located	localized	1544:1552	arg2	PoCda7					1533:1538	PoCda7	1533:1538	PoCda7	1533:1538	Subcellular localization experiments showed that PoCda7 was localized in the cell wall, and its fluorescence transferred to the EIHM and BIC when the rice blast fungus infected the rice leaf sheath, which was referred to as a candidate apoplastic effector in P. oryzae.
33744788	11	64	located	localized	1544:1552	arg1	wall					1566:1569	the cell wall	1557:1569	the cell wall	1557:1569	Subcellular localization experiments showed that PoCda7 was localized in the cell wall, and its fluorescence transferred to the EIHM and BIC when the rice blast fungus infected the rice leaf sheath, which was referred to as a candidate apoplastic effector in P. oryzae.
33744788	7	65	theme	sufficient	1088:1097	arg1	turgor					1099:1104	sufficient turgor	1088:1104	sufficient turgor in appressoria for invading the host surface	1088:1149	Glycerols are necessary to generate sufficient turgor in appressoria for invading the host surface.
33744788	1	66	theme	essential	116:124	arg1	role					126:129	an essential role	113:129	an essential role	113:129	The fungal cell wall plays an essential role in maintaining cellular integrity and facing complex and changing environmental conditions.
33744788	3	67	theme	cell	459:462	arg1	composition					469:479	cell wall composition	459:479	cell wall composition	459:479	Fungi developed infection-related changes in cell wall composition in co-evolution with nature to solve this problem.
33744788	9	68	from	death	1356:1360	arg1	benthamiana					1385:1395	Nicotiana benthamiana	1375:1395	Nicotiana benthamiana	1375:1395	PoCda7 inhibited the cell death of leaves in Nicotiana benthamiana.
33744788	2	69	theme	cell	387:390	arg1	wall					392:395	the fungal cell wall	376:395	the fungal cell wall	376:395	Whether a fungus successfully invades a host depends on whether it evades the plant's innate immune system, which recognizes the conserved components of the fungal cell wall, such as chitin.
33744788	6	70	theme	reactive	1027:1034	arg1	species					1043:1049	reactive oxygen species	1027:1049	reactive oxygen species	1027:1049	Phenotype analysis revealed that the knockout mutant of ΔPocda7 had no significant effect on fungal morphogenic development, including conidiation, germination, appressorial formation and cell wall of conidium and hyphae but was sensitive to reactive oxygen species.
33744788	2	71	theme	conserved	352:360	arg1	components					362:371	the conserved components	348:371	the conserved components	348:371	Whether a fungus successfully invades a host depends on whether it evades the plant's innate immune system, which recognizes the conserved components of the fungal cell wall, such as chitin.
33744788	2	71	theme	conserved	352:360	arg1	chitin					406:411	chitin	406:411	chitin	406:411	Whether a fungus successfully invades a host depends on whether it evades the plant's innate immune system, which recognizes the conserved components of the fungal cell wall, such as chitin.
33744788	5	72	from	deacetylase	750:760	arg1	Pyricularia					765:775	Pyricularia	765:775	Pyricularia	765:775	The present study revealed the functions of PoCda7, a chitin deacetylase in Pyricularia oryzae.
33744788	6	73	theme	conidium	986:993	arg1	conidiation					920:930	conidiation	920:930	conidiation	920:930	Phenotype analysis revealed that the knockout mutant of ΔPocda7 had no significant effect on fungal morphogenic development, including conidiation, germination, appressorial formation and cell wall of conidium and hyphae but was sensitive to reactive oxygen species.
33744788	6	73	theme	conidium	986:993	arg1	wall					978:981	cell wall	973:981	cell wall of conidium and hyphae	973:1004	Phenotype analysis revealed that the knockout mutant of ΔPocda7 had no significant effect on fungal morphogenic development, including conidiation, germination, appressorial formation and cell wall of conidium and hyphae but was sensitive to reactive oxygen species.
33744788	6	73	theme	conidium	986:993	arg1	germination					933:943	germination	933:943	germination	933:943	Phenotype analysis revealed that the knockout mutant of ΔPocda7 had no significant effect on fungal morphogenic development, including conidiation, germination, appressorial formation and cell wall of conidium and hyphae but was sensitive to reactive oxygen species.
33744788	6	73	theme	conidium	986:993	arg1	formation					959:967	appressorial formation	946:967	appressorial formation	946:967	Phenotype analysis revealed that the knockout mutant of ΔPocda7 had no significant effect on fungal morphogenic development, including conidiation, germination, appressorial formation and cell wall of conidium and hyphae but was sensitive to reactive oxygen species.
33744788	11	74	theme	apoplastic	1720:1729	arg1	effector					1731:1738	a candidate apoplastic effector	1708:1738	a candidate apoplastic effector in P. oryzae	1708:1751	Subcellular localization experiments showed that PoCda7 was localized in the cell wall, and its fluorescence transferred to the EIHM and BIC when the rice blast fungus infected the rice leaf sheath, which was referred to as a candidate apoplastic effector in P. oryzae.
33744788	11	74	theme	apoplastic	1720:1729	arg1	which					1683:1687	which	1683:1687	which	1683:1687	Subcellular localization experiments showed that PoCda7 was localized in the cell wall, and its fluorescence transferred to the EIHM and BIC when the rice blast fungus infected the rice leaf sheath, which was referred to as a candidate apoplastic effector in P. oryzae.
33744788	4	75	theme	chitin	585:590	arg1	CDA					605:607	CDA	605:607	CDA	605:607	One of the changes is the deacetylation of chitin by chitin deacetylase (CDA) to produce a polysaccharide that influences the infection of pathogenic fungi.
33744788	4	75	theme	chitin	585:590	arg1	deacetylase					592:602	chitin deacetylase	585:602	chitin deacetylase (CDA)	585:608	One of the changes is the deacetylation of chitin by chitin deacetylase (CDA) to produce a polysaccharide that influences the infection of pathogenic fungi.
32496183	6	0	from	acid	885:888	arg1	peptidoglycan					907:919	the cell-wall peptidoglycan	893:919	the cell-wall peptidoglycan	893:919	The organism was found to contain meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
32496183	2	1	theme	Gram-reaction-positive	98:119	arg1	bacterium					140:148	A Gram-reaction-positive, endospore-forming bacterium	96:148	A Gram-reaction-positive, endospore-forming bacterium	96:148	A Gram-reaction-positive, endospore-forming bacterium, designated strain P1T, was isolated from water samples collected from Pasinler Hot Spring and characterized using a polyphasic approach to clarify its taxonomic position.
32496183	9	2	dep	identified	1090:1099	arg1	 0					1113:1114	 0	1113:1114	 0	1113:1114	The major cellular fatty acids were identified as iso-C15 : 0, iso-C17 : 0 and anteiso-C17 : 0.
32496183	3	3	theme	Strain	322:327	arg1	P1T					329:331	Strain P1T	322:331	Strain P1T	322:331	Strain P1T was found to have chemotaxonomic and morphological characteristics consistent with its classification in the genus Bacillus.
32496183	3	4	from	classification	420:433	arg1	Bacillus					448:455	genus Bacillus	442:455	the genus Bacillus	438:455	Strain P1T was found to have chemotaxonomic and morphological characteristics consistent with its classification in the genus Bacillus.
32496183	7	5	theme	major	926:930	arg1	diphosphatidylglycerol					950:971	diphosphatidylglycerol	950:971	diphosphatidylglycerol	950:971	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol.
32496183	7	5	theme	major	926:930	arg1	lipids					938:943	The major polar lipids	922:943	The major polar lipids	922:943	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol.
32496183	4	6	theme	phylogenetic	673:684	arg1	trees					686:690	the phylogenetic trees	669:690	the phylogenetic trees based on 16S rRNA gene sequences	669:723	The strain shared the highest 16S rRNA gene sequence identity values with Bacillus thermolactis R-6488T (97.6 %) and Bacillus kokeshiiformis MO-04T (97.2 %) and formed a distinct clade with both type strains in the phylogenetic trees based on 16S rRNA gene sequences.
32496183	10	7	theme	strain	1216:1221	arg1	P1T					1223:1225	strain P1T	1216:1225	strain P1T	1216:1225	Based upon the consensus of phenotypic and phylogenetic analyses, strain P1T represents a novel species of the genus Bacillus, for which the name Bacillus pasinlerensis sp.
32496183	4	8	theme	Bacillus	575:582	arg1	MO-04T					599:604	Bacillus kokeshiiformis MO-04T	575:604	Bacillus kokeshiiformis MO-04T (97.2 %)	575:613	The strain shared the highest 16S rRNA gene sequence identity values with Bacillus thermolactis R-6488T (97.6 %) and Bacillus kokeshiiformis MO-04T (97.2 %) and formed a distinct clade with both type strains in the phylogenetic trees based on 16S rRNA gene sequences.
32496183	4	8	theme	Bacillus	575:582	arg1	%					612:612	97.2 %	607:612	97.2 %	607:612	The strain shared the highest 16S rRNA gene sequence identity values with Bacillus thermolactis R-6488T (97.6 %) and Bacillus kokeshiiformis MO-04T (97.2 %) and formed a distinct clade with both type strains in the phylogenetic trees based on 16S rRNA gene sequences.
32496183	4	9	dep	Bacillus	532:539	arg1	thermolactis					541:552	thermolactis	541:552	thermolactis	541:552	The strain shared the highest 16S rRNA gene sequence identity values with Bacillus thermolactis R-6488T (97.6 %) and Bacillus kokeshiiformis MO-04T (97.2 %) and formed a distinct clade with both type strains in the phylogenetic trees based on 16S rRNA gene sequences.
32496183	7	10	theme	polar	932:936	arg1	diphosphatidylglycerol					950:971	diphosphatidylglycerol	950:971	diphosphatidylglycerol	950:971	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol.
32496183	7	10	theme	polar	932:936	arg1	lipids					938:943	The major polar lipids	922:943	The major polar lipids	922:943	The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol.
32496183	12	11	theme	type	1345:1348	arg1	P1T					1360:1362	P1T	1360:1362	P1T (=DSM 107529T=CECT 9885T=NCCB 100674T)	1360:1401	The type strain is P1T (=DSM 107529T=CECT 9885T=NCCB 100674T).
32496183	12	11	theme	type	1345:1348	arg1	strain					1350:1355	The type strain	1341:1355	The type strain	1341:1355	The type strain is P1T (=DSM 107529T=CECT 9885T=NCCB 100674T).
32496183	10	12	theme	Bacillus	1296:1303	arg1	sp					1319:1320	name Bacillus pasinlerensis sp	1291:1320	the name Bacillus pasinlerensis sp	1287:1320	Based upon the consensus of phenotypic and phylogenetic analyses, strain P1T represents a novel species of the genus Bacillus, for which the name Bacillus pasinlerensis sp.
32496183	3	13	theme	chemotaxonomic	351:364	arg1	characteristics					384:398	chemotaxonomic and morphological characteristics	351:398	chemotaxonomic and morphological characteristics consistent with its classification in the genus Bacillus	351:455	Strain P1T was found to have chemotaxonomic and morphological characteristics consistent with its classification in the genus Bacillus.
32496183	4	14	theme	16S	488:490	arg1	rRNA					492:495	the highest 16S rRNA	476:495	the highest 16S rRNA gene sequence identity values	476:525	The strain shared the highest 16S rRNA gene sequence identity values with Bacillus thermolactis R-6488T (97.6 %) and Bacillus kokeshiiformis MO-04T (97.2 %) and formed a distinct clade with both type strains in the phylogenetic trees based on 16S rRNA gene sequences.
32496183	9	15	theme	major	1058:1062	arg1	acids					1079:1083	The major cellular fatty acids	1054:1083	The major cellular fatty acids	1054:1083	The major cellular fatty acids were identified as iso-C15 : 0, iso-C17 : 0 and anteiso-C17 : 0.
32496183	9	15	theme	major	1058:1062	arg1	iso-C15 					1104:1111	iso-C15 	1104:1111	iso-C15 	1104:1111	The major cellular fatty acids were identified as iso-C15 : 0, iso-C17 : 0 and anteiso-C17 : 0.
32496183	4	16	theme	highest	480:486	arg1	rRNA					492:495	the highest 16S rRNA	476:495	the highest 16S rRNA gene sequence identity values	476:525	The strain shared the highest 16S rRNA gene sequence identity values with Bacillus thermolactis R-6488T (97.6 %) and Bacillus kokeshiiformis MO-04T (97.2 %) and formed a distinct clade with both type strains in the phylogenetic trees based on 16S rRNA gene sequences.
32496183	2	17	theme	polyphasic	267:276	arg1	approach					278:285	a polyphasic approach	265:285	a polyphasic approach to clarify its taxonomic position	265:319	A Gram-reaction-positive, endospore-forming bacterium, designated strain P1T, was isolated from water samples collected from Pasinler Hot Spring and characterized using a polyphasic approach to clarify its taxonomic position.
32496183	9	18	theme	cellular	1064:1071	arg1	acids					1079:1083	The major cellular fatty acids	1054:1083	The major cellular fatty acids	1054:1083	The major cellular fatty acids were identified as iso-C15 : 0, iso-C17 : 0 and anteiso-C17 : 0.
32496183	9	18	theme	cellular	1064:1071	arg1	iso-C15 					1104:1111	iso-C15 	1104:1111	iso-C15 	1104:1111	The major cellular fatty acids were identified as iso-C15 : 0, iso-C17 : 0 and anteiso-C17 : 0.
32496183	5	19	theme	Strain	726:731	arg1	P1T					733:735	Strain P1T	726:735	Strain P1T	726:735	Strain P1T could grow optimally at 55 °C and in the presence of 2 % NaCl.
32496183	4	20	theme	gene	497:500	arg1	identity					511:518	the highest 16S rRNA gene sequence identity	476:518	the highest 16S rRNA gene sequence identity values	476:525	The strain shared the highest 16S rRNA gene sequence identity values with Bacillus thermolactis R-6488T (97.6 %) and Bacillus kokeshiiformis MO-04T (97.2 %) and formed a distinct clade with both type strains in the phylogenetic trees based on 16S rRNA gene sequences.
32496183	3	21	theme	consistent	400:409	arg1	characteristics					384:398	chemotaxonomic and morphological characteristics	351:398	chemotaxonomic and morphological characteristics consistent with its classification in the genus Bacillus	351:455	Strain P1T was found to have chemotaxonomic and morphological characteristics consistent with its classification in the genus Bacillus.
32496183	9	22	theme	fatty	1073:1077	arg1	acids					1079:1083	The major cellular fatty acids	1054:1083	The major cellular fatty acids	1054:1083	The major cellular fatty acids were identified as iso-C15 : 0, iso-C17 : 0 and anteiso-C17 : 0.
32496183	9	22	theme	fatty	1073:1077	arg1	iso-C15 					1104:1111	iso-C15 	1104:1111	iso-C15 	1104:1111	The major cellular fatty acids were identified as iso-C15 : 0, iso-C17 : 0 and anteiso-C17 : 0.
32496183	0	23	theme	pasinlerensis	9:21	arg1	sp					23:24	Bacillus pasinlerensis sp	0:24	Bacillus pasinlerensis sp.	0:25	Bacillus pasinlerensis sp.
32496183	4	24	theme	rRNA	492:495	arg1	identity					511:518	the highest 16S rRNA gene sequence identity	476:518	the highest 16S rRNA gene sequence identity values	476:525	The strain shared the highest 16S rRNA gene sequence identity values with Bacillus thermolactis R-6488T (97.6 %) and Bacillus kokeshiiformis MO-04T (97.2 %) and formed a distinct clade with both type strains in the phylogenetic trees based on 16S rRNA gene sequences.
32496183	10	25	theme	phylogenetic	1193:1204	arg1	analyses					1206:1213	phenotypic and phylogenetic analyses	1178:1213	phenotypic and phylogenetic analyses	1178:1213	Based upon the consensus of phenotypic and phylogenetic analyses, strain P1T represents a novel species of the genus Bacillus, for which the name Bacillus pasinlerensis sp.
32496183	1	26	theme	hot	74:76	arg1	spring					78:83	a hot spring	72:83	a hot spring in Turkey	72:93	nov., a thermophilic bacterium isolated from a hot spring in Turkey.
32496183	9	27	dep	 0	1113:1114	arg1	 0					1126:1127	 0	1126:1127	 0	1126:1127	The major cellular fatty acids were identified as iso-C15 : 0, iso-C17 : 0 and anteiso-C17 : 0.
32496183	9	27	dep	 0	1113:1114	arg1	 0					1146:1147	 0	1146:1147	 0	1146:1147	The major cellular fatty acids were identified as iso-C15 : 0, iso-C17 : 0 and anteiso-C17 : 0.
32496183	9	27	dep	 0	1113:1114	arg1	anteiso-C17 					1133:1144	anteiso-C17 	1133:1144	anteiso-C17 	1133:1144	The major cellular fatty acids were identified as iso-C15 : 0, iso-C17 : 0 and anteiso-C17 : 0.
32496183	2	28	attach	isolated	178:185	arg2	bacterium					140:148	A Gram-reaction-positive, endospore-forming bacterium	96:148	A Gram-reaction-positive, endospore-forming bacterium	96:148	A Gram-reaction-positive, endospore-forming bacterium, designated strain P1T, was isolated from water samples collected from Pasinler Hot Spring and characterized using a polyphasic approach to clarify its taxonomic position.
32496183	2	28	attach	isolated	178:185	arg1	samples					198:204	water samples	192:204	water samples collected from Pasinler Hot Spring	192:239	A Gram-reaction-positive, endospore-forming bacterium, designated strain P1T, was isolated from water samples collected from Pasinler Hot Spring and characterized using a polyphasic approach to clarify its taxonomic position.
32496183	0	29	theme	Bacillus	0:7	arg1	sp					23:24	Bacillus pasinlerensis sp	0:24	Bacillus pasinlerensis sp.	0:25	Bacillus pasinlerensis sp.
32496183	1	30	attach	isolated	58:65	arg2	bacterium					48:56	a thermophilic bacterium	33:56	a thermophilic bacterium isolated from a hot spring in Turkey	33:93	nov., a thermophilic bacterium isolated from a hot spring in Turkey.
32496183	1	30	attach	isolated	58:65	arg1	spring					78:83	a hot spring	72:83	a hot spring in Turkey	72:93	nov., a thermophilic bacterium isolated from a hot spring in Turkey.
32496183	8	31	theme	predominant	1003:1013	arg1	MK-7					1048:1051	MK-7	1048:1051	MK-7	1048:1051	The predominant menaquinone was determined to be MK-7.
32496183	8	31	theme	predominant	1003:1013	arg1	menaquinone					1015:1025	The predominant menaquinone	999:1025	The predominant menaquinone	999:1025	The predominant menaquinone was determined to be MK-7.
32496183	2	32	theme	taxonomic	302:310	arg1	position					312:319	its taxonomic position	298:319	its taxonomic position	298:319	A Gram-reaction-positive, endospore-forming bacterium, designated strain P1T, was isolated from water samples collected from Pasinler Hot Spring and characterized using a polyphasic approach to clarify its taxonomic position.
32496183	4	33	theme	type	653:656	arg1	strains					658:664	both type strains	648:664	both type strains	648:664	The strain shared the highest 16S rRNA gene sequence identity values with Bacillus thermolactis R-6488T (97.6 %) and Bacillus kokeshiiformis MO-04T (97.2 %) and formed a distinct clade with both type strains in the phylogenetic trees based on 16S rRNA gene sequences.
32496183	4	34	theme	sequence	502:509	arg1	identity					511:518	the highest 16S rRNA gene sequence identity	476:518	the highest 16S rRNA gene sequence identity values	476:525	The strain shared the highest 16S rRNA gene sequence identity values with Bacillus thermolactis R-6488T (97.6 %) and Bacillus kokeshiiformis MO-04T (97.2 %) and formed a distinct clade with both type strains in the phylogenetic trees based on 16S rRNA gene sequences.
32496183	4	35	theme	gene	710:713	arg1	sequences					715:723	16S rRNA gene sequences	701:723	16S rRNA gene sequences	701:723	The strain shared the highest 16S rRNA gene sequence identity values with Bacillus thermolactis R-6488T (97.6 %) and Bacillus kokeshiiformis MO-04T (97.2 %) and formed a distinct clade with both type strains in the phylogenetic trees based on 16S rRNA gene sequences.
32496183	10	36	theme	Bacillus	1267:1274	arg1	species					1246:1252	a novel species	1238:1252	a novel species	1238:1252	Based upon the consensus of phenotypic and phylogenetic analyses, strain P1T represents a novel species of the genus Bacillus, for which the name Bacillus pasinlerensis sp.
32496183	5	37	theme	%	792:792	arg1	NaCl					794:797	2 % NaCl	790:797	2 % NaCl	790:797	Strain P1T could grow optimally at 55 °C and in the presence of 2 % NaCl.
32496183	3	38	theme	morphological	370:382	arg1	characteristics					384:398	chemotaxonomic and morphological characteristics	351:398	chemotaxonomic and morphological characteristics consistent with its classification in the genus Bacillus	351:455	Strain P1T was found to have chemotaxonomic and morphological characteristics consistent with its classification in the genus Bacillus.
32496183	3	39	with	consistent	400:409	arg1	classification					420:433	its classification	416:433	its classification in the genus Bacillus	416:455	Strain P1T was found to have chemotaxonomic and morphological characteristics consistent with its classification in the genus Bacillus.
32496183	6	40	theme	diamino	877:883	arg1	acid					854:857	meso-diaminopimelic acid	834:857	meso-diaminopimelic acid	834:857	The organism was found to contain meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
32496183	6	40	theme	diamino	877:883	arg1	acid					885:888	the diagnostic diamino acid	862:888	the diagnostic diamino acid in the cell-wall peptidoglycan	862:919	The organism was found to contain meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
32496183	5	41	theme	NaCl	794:797	arg1	presence					778:785	the presence	774:785	the presence of 2 % NaCl	774:797	Strain P1T could grow optimally at 55 °C and in the presence of 2 % NaCl.
32496183	12	42	theme	107529T=CECT	1370:1381	arg1	100674T					1394:1400	=DSM 107529T=CECT 9885T=NCCB 100674T	1365:1400	=DSM 107529T=CECT 9885T=NCCB 100674T	1365:1400	The type strain is P1T (=DSM 107529T=CECT 9885T=NCCB 100674T).
32496183	12	42	theme	107529T=CECT	1370:1381	arg1	P1T					1360:1362	P1T	1360:1362	P1T (=DSM 107529T=CECT 9885T=NCCB 100674T)	1360:1401	The type strain is P1T (=DSM 107529T=CECT 9885T=NCCB 100674T).
32496183	2	43	theme	Hot	230:232	arg1	Spring					234:239	Pasinler Hot Spring	221:239	Pasinler Hot Spring	221:239	A Gram-reaction-positive, endospore-forming bacterium, designated strain P1T, was isolated from water samples collected from Pasinler Hot Spring and characterized using a polyphasic approach to clarify its taxonomic position.
32496183	10	44	theme	phenotypic	1178:1187	arg1	analyses					1206:1213	phenotypic and phylogenetic analyses	1178:1213	phenotypic and phylogenetic analyses	1178:1213	Based upon the consensus of phenotypic and phylogenetic analyses, strain P1T represents a novel species of the genus Bacillus, for which the name Bacillus pasinlerensis sp.
32496183	10	45	theme	analyses	1206:1213	arg1	consensus					1165:1173	the consensus	1161:1173	the consensus of phenotypic and phylogenetic analyses	1161:1213	Based upon the consensus of phenotypic and phylogenetic analyses, strain P1T represents a novel species of the genus Bacillus, for which the name Bacillus pasinlerensis sp.
32496183	2	46	theme	Pasinler	221:228	arg1	Spring					234:239	Pasinler Hot Spring	221:239	Pasinler Hot Spring	221:239	A Gram-reaction-positive, endospore-forming bacterium, designated strain P1T, was isolated from water samples collected from Pasinler Hot Spring and characterized using a polyphasic approach to clarify its taxonomic position.
32496183	10	47	theme	name	1291:1294	arg1	sp					1319:1320	name Bacillus pasinlerensis sp	1291:1320	the name Bacillus pasinlerensis sp	1287:1320	Based upon the consensus of phenotypic and phylogenetic analyses, strain P1T represents a novel species of the genus Bacillus, for which the name Bacillus pasinlerensis sp.
32496183	4	48	theme	distinct	628:635	arg1	clade					637:641	a distinct clade	626:641	a distinct clade with both type strains	626:664	The strain shared the highest 16S rRNA gene sequence identity values with Bacillus thermolactis R-6488T (97.6 %) and Bacillus kokeshiiformis MO-04T (97.2 %) and formed a distinct clade with both type strains in the phylogenetic trees based on 16S rRNA gene sequences.
32496183	10	49	theme	novel	1240:1244	arg1	species					1246:1252	a novel species	1238:1252	a novel species	1238:1252	Based upon the consensus of phenotypic and phylogenetic analyses, strain P1T represents a novel species of the genus Bacillus, for which the name Bacillus pasinlerensis sp.
32496183	4	50	theme	Bacillus	532:539	arg1	R-6488T					554:560	Bacillus thermolactis R-6488T	532:560	Bacillus thermolactis R-6488T (97.6 %)	532:569	The strain shared the highest 16S rRNA gene sequence identity values with Bacillus thermolactis R-6488T (97.6 %) and Bacillus kokeshiiformis MO-04T (97.2 %) and formed a distinct clade with both type strains in the phylogenetic trees based on 16S rRNA gene sequences.
32496183	4	50	theme	Bacillus	532:539	arg1	%					568:568	97.6 %	563:568	97.6 %	563:568	The strain shared the highest 16S rRNA gene sequence identity values with Bacillus thermolactis R-6488T (97.6 %) and Bacillus kokeshiiformis MO-04T (97.2 %) and formed a distinct clade with both type strains in the phylogenetic trees based on 16S rRNA gene sequences.
32496183	2	51	theme	strain	162:167	arg1	P1T					169:171	strain P1T	162:171	strain P1T	162:171	A Gram-reaction-positive, endospore-forming bacterium, designated strain P1T, was isolated from water samples collected from Pasinler Hot Spring and characterized using a polyphasic approach to clarify its taxonomic position.
32496183	6	52	theme	meso-diaminopimelic	834:852	arg1	acid					854:857	meso-diaminopimelic acid	834:857	meso-diaminopimelic acid	834:857	The organism was found to contain meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
32496183	6	52	theme	meso-diaminopimelic	834:852	arg1	acid					885:888	the diagnostic diamino acid	862:888	the diagnostic diamino acid in the cell-wall peptidoglycan	862:919	The organism was found to contain meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
32496183	4	53	with	clade	637:641	arg1	strains					658:664	both type strains	648:664	both type strains	648:664	The strain shared the highest 16S rRNA gene sequence identity values with Bacillus thermolactis R-6488T (97.6 %) and Bacillus kokeshiiformis MO-04T (97.2 %) and formed a distinct clade with both type strains in the phylogenetic trees based on 16S rRNA gene sequences.
32496183	4	54	dep	Bacillus	575:582	arg1	kokeshiiformis					584:597	kokeshiiformis	584:597	kokeshiiformis	584:597	The strain shared the highest 16S rRNA gene sequence identity values with Bacillus thermolactis R-6488T (97.6 %) and Bacillus kokeshiiformis MO-04T (97.2 %) and formed a distinct clade with both type strains in the phylogenetic trees based on 16S rRNA gene sequences.
32496183	2	55	dep	Gram-reaction-positive	98:119	arg1	endospore-forming					122:138	endospore-forming	122:138	endospore-forming	122:138	A Gram-reaction-positive, endospore-forming bacterium, designated strain P1T, was isolated from water samples collected from Pasinler Hot Spring and characterized using a polyphasic approach to clarify its taxonomic position.
32496183	12	56	theme	=DSM	1365:1368	arg1	100674T					1394:1400	=DSM 107529T=CECT 9885T=NCCB 100674T	1365:1400	=DSM 107529T=CECT 9885T=NCCB 100674T	1365:1400	The type strain is P1T (=DSM 107529T=CECT 9885T=NCCB 100674T).
32496183	12	56	theme	=DSM	1365:1368	arg1	P1T					1360:1362	P1T	1360:1362	P1T (=DSM 107529T=CECT 9885T=NCCB 100674T)	1360:1401	The type strain is P1T (=DSM 107529T=CECT 9885T=NCCB 100674T).
32496183	2	57	theme	water	192:196	arg1	samples					198:204	water samples	192:204	water samples collected from Pasinler Hot Spring	192:239	A Gram-reaction-positive, endospore-forming bacterium, designated strain P1T, was isolated from water samples collected from Pasinler Hot Spring and characterized using a polyphasic approach to clarify its taxonomic position.
32496183	3	58	contain	have	346:349	arg1	P1T					329:331	Strain P1T	322:331	Strain P1T	322:331	Strain P1T was found to have chemotaxonomic and morphological characteristics consistent with its classification in the genus Bacillus.
32496183	3	58	contain	have	346:349	arg2	characteristics					384:398	chemotaxonomic and morphological characteristics	351:398	chemotaxonomic and morphological characteristics consistent with its classification in the genus Bacillus	351:455	Strain P1T was found to have chemotaxonomic and morphological characteristics consistent with its classification in the genus Bacillus.
32496183	1	59	dep	bacterium	48:56	arg1	nov.					27:30	nov.	27:30	nov.	27:30	nov., a thermophilic bacterium isolated from a hot spring in Turkey.
32496183	6	60	theme	diagnostic	866:875	arg1	acid					854:857	meso-diaminopimelic acid	834:857	meso-diaminopimelic acid	834:857	The organism was found to contain meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
32496183	6	60	theme	diagnostic	866:875	arg1	acid					885:888	the diagnostic diamino acid	862:888	the diagnostic diamino acid in the cell-wall peptidoglycan	862:919	The organism was found to contain meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
32496183	5	61	theme	2 	790:791	arg1	%					792:792	%	792:792	%	792:792	Strain P1T could grow optimally at 55 °C and in the presence of 2 % NaCl.
32496183	4	62	theme	16S	701:703	arg1	sequences					715:723	16S rRNA gene sequences	701:723	16S rRNA gene sequences	701:723	The strain shared the highest 16S rRNA gene sequence identity values with Bacillus thermolactis R-6488T (97.6 %) and Bacillus kokeshiiformis MO-04T (97.2 %) and formed a distinct clade with both type strains in the phylogenetic trees based on 16S rRNA gene sequences.
32496183	4	63	theme	rRNA	705:708	arg1	sequences					715:723	16S rRNA gene sequences	701:723	16S rRNA gene sequences	701:723	The strain shared the highest 16S rRNA gene sequence identity values with Bacillus thermolactis R-6488T (97.6 %) and Bacillus kokeshiiformis MO-04T (97.2 %) and formed a distinct clade with both type strains in the phylogenetic trees based on 16S rRNA gene sequences.
32496183	6	64	contain	contain	826:832	arg2	acid					885:888	the diagnostic diamino acid	862:888	the diagnostic diamino acid in the cell-wall peptidoglycan	862:919	The organism was found to contain meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
32496183	6	64	contain	contain	826:832	arg1	organism					804:811	The organism	800:811	The organism	800:811	The organism was found to contain meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
32496183	6	64	contain	contain	826:832	arg2	acid					854:857	meso-diaminopimelic acid	834:857	meso-diaminopimelic acid	834:857	The organism was found to contain meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
32496183	1	65	from	spring	78:83	arg1	Turkey					88:93	Turkey	88:93	Turkey	88:93	nov., a thermophilic bacterium isolated from a hot spring in Turkey.
32496183	4	66	theme	identity	511:518	arg1	values					520:525	the highest 16S rRNA gene sequence identity values	476:525	the highest 16S rRNA gene sequence identity values	476:525	The strain shared the highest 16S rRNA gene sequence identity values with Bacillus thermolactis R-6488T (97.6 %) and Bacillus kokeshiiformis MO-04T (97.2 %) and formed a distinct clade with both type strains in the phylogenetic trees based on 16S rRNA gene sequences.
32496183	1	67	theme	thermophilic	35:46	arg1	bacterium					48:56	a thermophilic bacterium	33:56	a thermophilic bacterium isolated from a hot spring in Turkey	33:93	nov., a thermophilic bacterium isolated from a hot spring in Turkey.
32496183	10	68	theme	pasinlerensis	1305:1317	arg1	sp					1319:1320	name Bacillus pasinlerensis sp	1291:1320	the name Bacillus pasinlerensis sp	1287:1320	Based upon the consensus of phenotypic and phylogenetic analyses, strain P1T represents a novel species of the genus Bacillus, for which the name Bacillus pasinlerensis sp.
32496183	12	69	theme	9885T=NCCB	1383:1392	arg1	100674T					1394:1400	=DSM 107529T=CECT 9885T=NCCB 100674T	1365:1400	=DSM 107529T=CECT 9885T=NCCB 100674T	1365:1400	The type strain is P1T (=DSM 107529T=CECT 9885T=NCCB 100674T).
32496183	12	69	theme	9885T=NCCB	1383:1392	arg1	P1T					1360:1362	P1T	1360:1362	P1T (=DSM 107529T=CECT 9885T=NCCB 100674T)	1360:1401	The type strain is P1T (=DSM 107529T=CECT 9885T=NCCB 100674T).
32496183	6	70	theme	cell-wall	897:905	arg1	peptidoglycan					907:919	the cell-wall peptidoglycan	893:919	the cell-wall peptidoglycan	893:919	The organism was found to contain meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan.
32877604	0	0	theme	putida	87:92	arg1	Strains					64:70	Genome-Reduced Strains	49:70	Genome-Reduced Strains of Pseudomonas putida	49:92	Surface Display of Designer Protein Scaffolds on Genome-Reduced Strains of Pseudomonas putida.
32877604	4	1	theme	cohesin	691:697	arg1	domains					707:713	cohesin binding domains	691:713	cohesin binding domains from divergent cellulolytic bacterial species	691:759	In this work, we report an essential step in this endeavor-a display of designer enzyme-anchoring protein "scaffoldins", encompassing cohesin binding domains from divergent cellulolytic bacterial species on the P. putida surface.
32877604	5	2	from	differences	863:873	arg1	composition					882:892	the composition	878:892	the composition of the outer membrane	878:914	Two P. putida chassis strains, EM42 and EM371, with streamlined genomes and differences in the composition of the outer membrane were employed in this study.
32877604	2	3	theme	such	358:361	arg1	substrates					371:380	such complex substrates	358:380	such complex substrates	358:380	However, P. putida on its own cannot make much use of such complex substrates, mainly because it lacks an efficient extracellular depolymerizing apparatus.
32877604	1	4	theme	lignocellulosic	266:280	arg1	residues					282:289	lignocellulosic residues	266:289	lignocellulosic residues	266:289	The bacterium Pseudomonas putida KT2440 is gaining considerable interest as a microbial platform for biotechnological valorization of polymeric organic materials, such as lignocellulosic residues or plastics.
32877604	0	5	theme	Pseudomonas	75:85	arg1	putida					87:92	Pseudomonas putida	75:92	Pseudomonas putida	75:92	Surface Display of Designer Protein Scaffolds on Genome-Reduced Strains of Pseudomonas putida.
32877604	3	6	theme	cellulosome	521:531	arg1	strategy					533:540	a recombinant cellulosome strategy	507:540	a recombinant cellulosome strategy for this host	507:554	We seek to address this limitation by adopting a recombinant cellulosome strategy for this host.
32877604	4	7	from	step	594:597	arg1	display					618:624	this endeavor-a display	602:624	this endeavor-a display of designer enzyme-anchoring protein "scaffoldins"	602:675	In this work, we report an essential step in this endeavor-a display of designer enzyme-anchoring protein "scaffoldins", encompassing cohesin binding domains from divergent cellulolytic bacterial species on the P. putida surface.
32877604	7	8	theme	bacteria	1348:1355	arg1	envelope					1322:1329	the envelope	1318:1329	the envelope of Gram-negative bacteria	1318:1355	Our results not only highlight the value of cell surface engineering for presentation of recombinant proteins on the envelope of Gram-negative bacteria but also pave the way toward designer cellulosome strategies tailored for P. putida.
32877604	6	9	theme	proteins	1195:1202	arg1	attachment					1142:1151	extracellular attachment	1128:1151	extracellular attachment of chimeric β-glucosidase and fluorescent proteins	1128:1202	Scaffoldin variants were optimally delivered to their surface with one of four tested autotransporter systems (Ag43 from Escherichia coli), and the efficient display was confirmed by extracellular attachment of chimeric β-glucosidase and fluorescent proteins.
32877604	4	10	theme	scaffoldins	664:674	arg1	"					675:675	designer enzyme-anchoring protein "scaffoldins"	629:675	designer enzyme-anchoring protein "scaffoldins"	629:675	In this work, we report an essential step in this endeavor-a display of designer enzyme-anchoring protein "scaffoldins", encompassing cohesin binding domains from divergent cellulolytic bacterial species on the P. putida surface.
32877604	7	11	from	presentation	1278:1289	arg1	envelope					1322:1329	the envelope	1318:1329	the envelope of Gram-negative bacteria	1318:1355	Our results not only highlight the value of cell surface engineering for presentation of recombinant proteins on the envelope of Gram-negative bacteria but also pave the way toward designer cellulosome strategies tailored for P. putida.
32877604	5	12	with	strains	809:815	arg1	genomes					851:857	streamlined genomes	839:857	streamlined genomes	839:857	Two P. putida chassis strains, EM42 and EM371, with streamlined genomes and differences in the composition of the outer membrane were employed in this study.
32877604	7	13	theme	cell	1249:1252	arg1	engineering					1262:1272	cell surface engineering	1249:1272	cell surface engineering	1249:1272	Our results not only highlight the value of cell surface engineering for presentation of recombinant proteins on the envelope of Gram-negative bacteria but also pave the way toward designer cellulosome strategies tailored for P. putida.
32877604	3	14	theme	recombinant	509:519	arg1	strategy					533:540	a recombinant cellulosome strategy	507:540	a recombinant cellulosome strategy for this host	507:554	We seek to address this limitation by adopting a recombinant cellulosome strategy for this host.
32877604	4	15	theme	essential	584:592	arg1	step					594:597	an essential step	581:597	an essential step in this endeavor-a display of designer enzyme-anchoring protein "scaffoldins"	581:675	In this work, we report an essential step in this endeavor-a display of designer enzyme-anchoring protein "scaffoldins", encompassing cohesin binding domains from divergent cellulolytic bacterial species on the P. putida surface.
32877604	4	16	theme	binding	699:705	arg1	domains					707:713	cohesin binding domains	691:713	cohesin binding domains from divergent cellulolytic bacterial species	691:759	In this work, we report an essential step in this endeavor-a display of designer enzyme-anchoring protein "scaffoldins", encompassing cohesin binding domains from divergent cellulolytic bacterial species on the P. putida surface.
32877604	5	17	theme	membrane	907:914	arg1	composition					882:892	the composition	878:892	the composition of the outer membrane	878:914	Two P. putida chassis strains, EM42 and EM371, with streamlined genomes and differences in the composition of the outer membrane were employed in this study.
32877604	6	18	from	coli	1078:1081	arg1	Ag43					1056:1059	Ag43	1056:1059	Ag43 from Escherichia coli	1056:1081	Scaffoldin variants were optimally delivered to their surface with one of four tested autotransporter systems (Ag43 from Escherichia coli), and the efficient display was confirmed by extracellular attachment of chimeric β-glucosidase and fluorescent proteins.
32877604	6	19	theme	tested	1024:1029	arg1	systems					1047:1053	four tested autotransporter systems	1019:1053	four tested autotransporter systems (Ag43 from Escherichia coli)	1019:1082	Scaffoldin variants were optimally delivered to their surface with one of four tested autotransporter systems (Ag43 from Escherichia coli), and the efficient display was confirmed by extracellular attachment of chimeric β-glucosidase and fluorescent proteins.
32877604	1	20	theme	microbial	173:181	arg1	interest					159:166	considerable interest	146:166	considerable interest	146:166	The bacterium Pseudomonas putida KT2440 is gaining considerable interest as a microbial platform for biotechnological valorization of polymeric organic materials, such as lignocellulosic residues or plastics.
32877604	1	20	theme	microbial	173:181	arg1	platform					183:190	a microbial platform	171:190	a microbial platform for biotechnological valorization of polymeric organic materials, such as lignocellulosic residues or plastics	171:301	The bacterium Pseudomonas putida KT2440 is gaining considerable interest as a microbial platform for biotechnological valorization of polymeric organic materials, such as lignocellulosic residues or plastics.
32877604	5	21	theme	outer	901:905	arg1	membrane					907:914	the outer membrane	897:914	the outer membrane	897:914	Two P. putida chassis strains, EM42 and EM371, with streamlined genomes and differences in the composition of the outer membrane were employed in this study.
32877604	6	22	theme	β-glucosidase	1165:1177	arg1	proteins					1195:1202	chimeric β-glucosidase and fluorescent proteins	1156:1202	chimeric β-glucosidase and fluorescent proteins	1156:1202	Scaffoldin variants were optimally delivered to their surface with one of four tested autotransporter systems (Ag43 from Escherichia coli), and the efficient display was confirmed by extracellular attachment of chimeric β-glucosidase and fluorescent proteins.
32877604	7	23	theme	proteins	1306:1313	arg1	presentation					1278:1289	presentation	1278:1289	presentation of recombinant proteins on the envelope of Gram-negative bacteria	1278:1355	Our results not only highlight the value of cell surface engineering for presentation of recombinant proteins on the envelope of Gram-negative bacteria but also pave the way toward designer cellulosome strategies tailored for P. putida.
32877604	7	24	theme	surface	1254:1260	arg1	engineering					1262:1272	cell surface engineering	1249:1272	cell surface engineering	1249:1272	Our results not only highlight the value of cell surface engineering for presentation of recombinant proteins on the envelope of Gram-negative bacteria but also pave the way toward designer cellulosome strategies tailored for P. putida.
32877604	6	25	theme	chimeric	1156:1163	arg1	proteins					1195:1202	chimeric β-glucosidase and fluorescent proteins	1156:1202	chimeric β-glucosidase and fluorescent proteins	1156:1202	Scaffoldin variants were optimally delivered to their surface with one of four tested autotransporter systems (Ag43 from Escherichia coli), and the efficient display was confirmed by extracellular attachment of chimeric β-glucosidase and fluorescent proteins.
32877604	0	26	theme	Surface	0:6	arg1	Display					8:14	Surface Display	0:14	Surface Display of Designer Protein	0:34	Surface Display of Designer Protein Scaffolds on Genome-Reduced Strains of Pseudomonas putida.
32877604	6	27	theme	systems	1047:1053	arg1	systems					1047:1053	four tested autotransporter systems	1019:1053	four tested autotransporter systems (Ag43 from Escherichia coli)	1019:1082	Scaffoldin variants were optimally delivered to their surface with one of four tested autotransporter systems (Ag43 from Escherichia coli), and the efficient display was confirmed by extracellular attachment of chimeric β-glucosidase and fluorescent proteins.
32877604	6	27	theme	systems	1047:1053	arg1	one					1012:1014	one	1012:1014	one	1012:1014	Scaffoldin variants were optimally delivered to their surface with one of four tested autotransporter systems (Ag43 from Escherichia coli), and the efficient display was confirmed by extracellular attachment of chimeric β-glucosidase and fluorescent proteins.
32877604	1	28	theme	biotechnological	196:211	arg1	valorization					213:224	biotechnological valorization	196:224	biotechnological valorization of polymeric organic materials, such as lignocellulosic residues or plastics	196:301	The bacterium Pseudomonas putida KT2440 is gaining considerable interest as a microbial platform for biotechnological valorization of polymeric organic materials, such as lignocellulosic residues or plastics.
32877604	0	29	theme	Designer	19:26	arg1	Protein					28:34	Designer Protein	19:34	Designer Protein	19:34	Surface Display of Designer Protein Scaffolds on Genome-Reduced Strains of Pseudomonas putida.
32877604	5	30	theme	streamlined	839:849	arg1	genomes					851:857	streamlined genomes	839:857	streamlined genomes	839:857	Two P. putida chassis strains, EM42 and EM371, with streamlined genomes and differences in the composition of the outer membrane were employed in this study.
32877604	4	31	from	species	753:759	arg1	domains					707:713	cohesin binding domains	691:713	cohesin binding domains from divergent cellulolytic bacterial species	691:759	In this work, we report an essential step in this endeavor-a display of designer enzyme-anchoring protein "scaffoldins", encompassing cohesin binding domains from divergent cellulolytic bacterial species on the P. putida surface.
32877604	1	32	theme	bacterium	99:107	arg1	putida					121:126	bacterium Pseudomonas putida KT2440	99:133	The bacterium Pseudomonas putida KT2440	95:133	The bacterium Pseudomonas putida KT2440 is gaining considerable interest as a microbial platform for biotechnological valorization of polymeric organic materials, such as lignocellulosic residues or plastics.
32877604	2	33	theme	extracellular	420:432	arg1	apparatus					449:457	an efficient extracellular depolymerizing apparatus	407:457	an efficient extracellular depolymerizing apparatus	407:457	However, P. putida on its own cannot make much use of such complex substrates, mainly because it lacks an efficient extracellular depolymerizing apparatus.
32877604	2	34	theme	depolymerizing	434:447	arg1	apparatus					449:457	an efficient extracellular depolymerizing apparatus	407:457	an efficient extracellular depolymerizing apparatus	407:457	However, P. putida on its own cannot make much use of such complex substrates, mainly because it lacks an efficient extracellular depolymerizing apparatus.
32877604	1	35	theme	Pseudomonas	109:119	arg1	putida					121:126	bacterium Pseudomonas putida KT2440	99:133	The bacterium Pseudomonas putida KT2440	95:133	The bacterium Pseudomonas putida KT2440 is gaining considerable interest as a microbial platform for biotechnological valorization of polymeric organic materials, such as lignocellulosic residues or plastics.
32877604	2	36	theme	efficient	410:418	arg1	apparatus					449:457	an efficient extracellular depolymerizing apparatus	407:457	an efficient extracellular depolymerizing apparatus	407:457	However, P. putida on its own cannot make much use of such complex substrates, mainly because it lacks an efficient extracellular depolymerizing apparatus.
32877604	5	37	theme	P.	791:792	arg1	EM42					818:821	EM42	818:821	EM42	818:821	Two P. putida chassis strains, EM42 and EM371, with streamlined genomes and differences in the composition of the outer membrane were employed in this study.
32877604	5	37	theme	P.	791:792	arg1	strains					809:815	Two P. putida chassis strains	787:815	Two P. putida chassis strains	787:815	Two P. putida chassis strains, EM42 and EM371, with streamlined genomes and differences in the composition of the outer membrane were employed in this study.
32877604	5	37	theme	P.	791:792	arg1	EM371					827:831	EM371	827:831	EM371	827:831	Two P. putida chassis strains, EM42 and EM371, with streamlined genomes and differences in the composition of the outer membrane were employed in this study.
32877604	7	38	theme	recombinant	1294:1304	arg1	proteins					1306:1313	recombinant proteins	1294:1313	recombinant proteins	1294:1313	Our results not only highlight the value of cell surface engineering for presentation of recombinant proteins on the envelope of Gram-negative bacteria but also pave the way toward designer cellulosome strategies tailored for P. putida.
32877604	4	39	theme	protein	655:661	arg1	"					675:675	designer enzyme-anchoring protein "scaffoldins"	629:675	designer enzyme-anchoring protein "scaffoldins"	629:675	In this work, we report an essential step in this endeavor-a display of designer enzyme-anchoring protein "scaffoldins", encompassing cohesin binding domains from divergent cellulolytic bacterial species on the P. putida surface.
32877604	7	40	theme	cellulosome	1395:1405	arg1	strategies					1407:1416	designer cellulosome strategies	1386:1416	designer cellulosome strategies tailored for P. putida	1386:1439	Our results not only highlight the value of cell surface engineering for presentation of recombinant proteins on the envelope of Gram-negative bacteria but also pave the way toward designer cellulosome strategies tailored for P. putida.
32877604	4	41	theme	"	675:675	arg1	display					618:624	this endeavor-a display	602:624	this endeavor-a display of designer enzyme-anchoring protein "scaffoldins"	602:675	In this work, we report an essential step in this endeavor-a display of designer enzyme-anchoring protein "scaffoldins", encompassing cohesin binding domains from divergent cellulolytic bacterial species on the P. putida surface.
32877604	1	42	theme	polymeric	229:237	arg1	residues					282:289	lignocellulosic residues	266:289	lignocellulosic residues	266:289	The bacterium Pseudomonas putida KT2440 is gaining considerable interest as a microbial platform for biotechnological valorization of polymeric organic materials, such as lignocellulosic residues or plastics.
32877604	1	42	theme	polymeric	229:237	arg1	materials					247:255	polymeric organic materials	229:255	polymeric organic materials	229:255	The bacterium Pseudomonas putida KT2440 is gaining considerable interest as a microbial platform for biotechnological valorization of polymeric organic materials, such as lignocellulosic residues or plastics.
32877604	1	42	theme	polymeric	229:237	arg1	plastics					294:301	plastics	294:301	plastics	294:301	The bacterium Pseudomonas putida KT2440 is gaining considerable interest as a microbial platform for biotechnological valorization of polymeric organic materials, such as lignocellulosic residues or plastics.
32877604	0	43	theme	Protein	28:34	arg1	Display					8:14	Surface Display	0:14	Surface Display of Designer Protein	0:34	Surface Display of Designer Protein Scaffolds on Genome-Reduced Strains of Pseudomonas putida.
32877604	6	44	theme	Scaffoldin	945:954	arg1	variants					956:963	Scaffoldin variants	945:963	Scaffoldin variants	945:963	Scaffoldin variants were optimally delivered to their surface with one of four tested autotransporter systems (Ag43 from Escherichia coli), and the efficient display was confirmed by extracellular attachment of chimeric β-glucosidase and fluorescent proteins.
32877604	1	45	theme	organic	239:245	arg1	residues					282:289	lignocellulosic residues	266:289	lignocellulosic residues	266:289	The bacterium Pseudomonas putida KT2440 is gaining considerable interest as a microbial platform for biotechnological valorization of polymeric organic materials, such as lignocellulosic residues or plastics.
32877604	1	45	theme	organic	239:245	arg1	materials					247:255	polymeric organic materials	229:255	polymeric organic materials	229:255	The bacterium Pseudomonas putida KT2440 is gaining considerable interest as a microbial platform for biotechnological valorization of polymeric organic materials, such as lignocellulosic residues or plastics.
32877604	1	45	theme	organic	239:245	arg1	plastics					294:301	plastics	294:301	plastics	294:301	The bacterium Pseudomonas putida KT2440 is gaining considerable interest as a microbial platform for biotechnological valorization of polymeric organic materials, such as lignocellulosic residues or plastics.
32877604	0	46	theme	Genome-Reduced	49:62	arg1	Strains					64:70	Genome-Reduced Strains	49:70	Genome-Reduced Strains of Pseudomonas putida	49:92	Surface Display of Designer Protein Scaffolds on Genome-Reduced Strains of Pseudomonas putida.
32877604	6	47	theme	autotransporter	1031:1045	arg1	systems					1047:1053	four tested autotransporter systems	1019:1053	four tested autotransporter systems (Ag43 from Escherichia coli)	1019:1082	Scaffoldin variants were optimally delivered to their surface with one of four tested autotransporter systems (Ag43 from Escherichia coli), and the efficient display was confirmed by extracellular attachment of chimeric β-glucosidase and fluorescent proteins.
32877604	6	48	theme	efficient	1093:1101	arg1	display					1103:1109	the efficient display	1089:1109	the efficient display	1089:1109	Scaffoldin variants were optimally delivered to their surface with one of four tested autotransporter systems (Ag43 from Escherichia coli), and the efficient display was confirmed by extracellular attachment of chimeric β-glucosidase and fluorescent proteins.
32877604	4	49	theme	divergent	720:728	arg1	species					753:759	divergent cellulolytic bacterial species	720:759	divergent cellulolytic bacterial species	720:759	In this work, we report an essential step in this endeavor-a display of designer enzyme-anchoring protein "scaffoldins", encompassing cohesin binding domains from divergent cellulolytic bacterial species on the P. putida surface.
32877604	6	50	theme	fluorescent	1183:1193	arg1	proteins					1195:1202	chimeric β-glucosidase and fluorescent proteins	1156:1202	chimeric β-glucosidase and fluorescent proteins	1156:1202	Scaffoldin variants were optimally delivered to their surface with one of four tested autotransporter systems (Ag43 from Escherichia coli), and the efficient display was confirmed by extracellular attachment of chimeric β-glucosidase and fluorescent proteins.
32877604	7	51	theme	Gram-negative	1334:1346	arg1	bacteria					1348:1355	Gram-negative bacteria	1334:1355	Gram-negative bacteria	1334:1355	Our results not only highlight the value of cell surface engineering for presentation of recombinant proteins on the envelope of Gram-negative bacteria but also pave the way toward designer cellulosome strategies tailored for P. putida.
32877604	7	52	theme	designer	1386:1393	arg1	strategies					1407:1416	designer cellulosome strategies	1386:1416	designer cellulosome strategies tailored for P. putida	1386:1439	Our results not only highlight the value of cell surface engineering for presentation of recombinant proteins on the envelope of Gram-negative bacteria but also pave the way toward designer cellulosome strategies tailored for P. putida.
32877604	5	53	from	strains	809:815	arg1	composition					882:892	the composition	878:892	the composition of the outer membrane	878:914	Two P. putida chassis strains, EM42 and EM371, with streamlined genomes and differences in the composition of the outer membrane were employed in this study.
32877604	2	54	theme	much	346:349	arg1	use					351:353	much use	346:353	much use of such complex substrates	346:380	However, P. putida on its own cannot make much use of such complex substrates, mainly because it lacks an efficient extracellular depolymerizing apparatus.
32877604	7	55	theme	engineering	1262:1272	arg1	value					1240:1244	the value	1236:1244	the value of cell surface engineering for presentation of recombinant proteins on the envelope of Gram-negative bacteria	1236:1355	Our results not only highlight the value of cell surface engineering for presentation of recombinant proteins on the envelope of Gram-negative bacteria but also pave the way toward designer cellulosome strategies tailored for P. putida.
32877604	1	56	theme	considerable	146:157	arg1	interest					159:166	considerable interest	146:166	considerable interest	146:166	The bacterium Pseudomonas putida KT2440 is gaining considerable interest as a microbial platform for biotechnological valorization of polymeric organic materials, such as lignocellulosic residues or plastics.
32877604	1	56	theme	considerable	146:157	arg1	platform					183:190	a microbial platform	171:190	a microbial platform for biotechnological valorization of polymeric organic materials, such as lignocellulosic residues or plastics	171:301	The bacterium Pseudomonas putida KT2440 is gaining considerable interest as a microbial platform for biotechnological valorization of polymeric organic materials, such as lignocellulosic residues or plastics.
32877604	4	57	theme	bacterial	743:751	arg1	species					753:759	divergent cellulolytic bacterial species	720:759	divergent cellulolytic bacterial species	720:759	In this work, we report an essential step in this endeavor-a display of designer enzyme-anchoring protein "scaffoldins", encompassing cohesin binding domains from divergent cellulolytic bacterial species on the P. putida surface.
32877604	4	58	theme	P.	768:769	arg1	surface					778:784	the P. putida surface	764:784	the P. putida surface	764:784	In this work, we report an essential step in this endeavor-a display of designer enzyme-anchoring protein "scaffoldins", encompassing cohesin binding domains from divergent cellulolytic bacterial species on the P. putida surface.
32877604	1	59	theme	materials	247:255	arg1	valorization					213:224	biotechnological valorization	196:224	biotechnological valorization of polymeric organic materials, such as lignocellulosic residues or plastics	196:301	The bacterium Pseudomonas putida KT2440 is gaining considerable interest as a microbial platform for biotechnological valorization of polymeric organic materials, such as lignocellulosic residues or plastics.
32877604	5	60	with	differences	863:873	arg1	genomes					851:857	streamlined genomes	839:857	streamlined genomes	839:857	Two P. putida chassis strains, EM42 and EM371, with streamlined genomes and differences in the composition of the outer membrane were employed in this study.
32877604	6	61	dep	systems	1047:1053	arg1	Ag43					1056:1059	Ag43	1056:1059	Ag43 from Escherichia coli	1056:1081	Scaffoldin variants were optimally delivered to their surface with one of four tested autotransporter systems (Ag43 from Escherichia coli), and the efficient display was confirmed by extracellular attachment of chimeric β-glucosidase and fluorescent proteins.
32877604	4	62	theme	cellulolytic	730:741	arg1	species					753:759	divergent cellulolytic bacterial species	720:759	divergent cellulolytic bacterial species	720:759	In this work, we report an essential step in this endeavor-a display of designer enzyme-anchoring protein "scaffoldins", encompassing cohesin binding domains from divergent cellulolytic bacterial species on the P. putida surface.
32877604	4	63	theme	enzyme-anchoring	638:653	arg1	"					675:675	designer enzyme-anchoring protein "scaffoldins"	629:675	designer enzyme-anchoring protein "scaffoldins"	629:675	In this work, we report an essential step in this endeavor-a display of designer enzyme-anchoring protein "scaffoldins", encompassing cohesin binding domains from divergent cellulolytic bacterial species on the P. putida surface.
32877604	4	64	theme	designer	629:636	arg1	"					675:675	designer enzyme-anchoring protein "scaffoldins"	629:675	designer enzyme-anchoring protein "scaffoldins"	629:675	In this work, we report an essential step in this endeavor-a display of designer enzyme-anchoring protein "scaffoldins", encompassing cohesin binding domains from divergent cellulolytic bacterial species on the P. putida surface.
32877604	2	65	theme	substrates	371:380	arg1	use					351:353	much use	346:353	much use of such complex substrates	346:380	However, P. putida on its own cannot make much use of such complex substrates, mainly because it lacks an efficient extracellular depolymerizing apparatus.
32877604	5	66	theme	chassis	801:807	arg1	EM42					818:821	EM42	818:821	EM42	818:821	Two P. putida chassis strains, EM42 and EM371, with streamlined genomes and differences in the composition of the outer membrane were employed in this study.
32877604	5	66	theme	chassis	801:807	arg1	strains					809:815	Two P. putida chassis strains	787:815	Two P. putida chassis strains	787:815	Two P. putida chassis strains, EM42 and EM371, with streamlined genomes and differences in the composition of the outer membrane were employed in this study.
32877604	5	66	theme	chassis	801:807	arg1	EM371					827:831	EM371	827:831	EM371	827:831	Two P. putida chassis strains, EM42 and EM371, with streamlined genomes and differences in the composition of the outer membrane were employed in this study.
32877604	6	67	theme	extracellular	1128:1140	arg1	attachment					1142:1151	extracellular attachment	1128:1151	extracellular attachment of chimeric β-glucosidase and fluorescent proteins	1128:1202	Scaffoldin variants were optimally delivered to their surface with one of four tested autotransporter systems (Ag43 from Escherichia coli), and the efficient display was confirmed by extracellular attachment of chimeric β-glucosidase and fluorescent proteins.
32877604	6	68	with	surface	999:1005	arg1	systems					1047:1053	four tested autotransporter systems	1019:1053	four tested autotransporter systems (Ag43 from Escherichia coli)	1019:1082	Scaffoldin variants were optimally delivered to their surface with one of four tested autotransporter systems (Ag43 from Escherichia coli), and the efficient display was confirmed by extracellular attachment of chimeric β-glucosidase and fluorescent proteins.
32877604	6	68	with	surface	999:1005	arg1	one					1012:1014	one	1012:1014	one	1012:1014	Scaffoldin variants were optimally delivered to their surface with one of four tested autotransporter systems (Ag43 from Escherichia coli), and the efficient display was confirmed by extracellular attachment of chimeric β-glucosidase and fluorescent proteins.
32877604	2	69	theme	complex	363:369	arg1	substrates					371:380	such complex substrates	358:380	such complex substrates	358:380	However, P. putida on its own cannot make much use of such complex substrates, mainly because it lacks an efficient extracellular depolymerizing apparatus.
32877604	4	70	theme	endeavor-a	607:616	arg1	display					618:624	this endeavor-a display	602:624	this endeavor-a display of designer enzyme-anchoring protein "scaffoldins"	602:675	In this work, we report an essential step in this endeavor-a display of designer enzyme-anchoring protein "scaffoldins", encompassing cohesin binding domains from divergent cellulolytic bacterial species on the P. putida surface.
32808115	3	0	theme	precise	317:323	arg1	function					325:332	The precise function	313:332	The precise function of F. plautii	313:346	The precise function of F. plautii remains unclear.
32808115	12	1	from	inhibition	1397:1406	arg1	environments					1444:1455	inflammatory environments	1431:1455	inflammatory environments	1431:1455	F. plautii may be involved in inhibition of TNF-α expression in inflammatory environments.
32808115	8	2	theme	epididymal	914:923	arg1	tissue					933:938	the epididymal adipose tissue	910:938	the epididymal adipose tissue of HFD-fed obese mice (HFD + F. plautii)	910:979	The oral administration of F. plautii attenuated the increase in TNF-α transcription otherwise seen in the epididymal adipose tissue of HFD-fed obese mice (HFD + F. plautii).
32808115	12	3	theme	F.	1367:1368	arg1	plautii					1370:1376	F. plautii	1367:1376	F. plautii	1367:1376	F. plautii may be involved in inhibition of TNF-α expression in inflammatory environments.
32808115	0	4	theme	adipose	89:95	arg1	tissue					97:102	obese adipose tissue	83:102	obese adipose tissue	83:102	Oral administration of Flavonifractor plautii attenuates inflammatory responses in obese adipose tissue.
32808115	8	5	theme	oral	811:814	arg1	administration					816:829	The oral administration	807:829	The oral administration of F. plautii	807:843	The oral administration of F. plautii attenuated the increase in TNF-α transcription otherwise seen in the epididymal adipose tissue of HFD-fed obese mice (HFD + F. plautii).
32808115	7	6	theme	final	706:710	arg1	weeks					716:720	the final two weeks	702:720	the final two weeks on the HFD	702:731	During the final two weeks on the HFD, the animals were administered with F. plautii by once-daily oral gavage.
32808115	11	7	theme	adipose	1351:1357	arg1	tissue					1359:1364	adipose tissue	1351:1364	adipose tissue	1351:1364	Obesity is closely associated with the development of inflammation in adipose tissue.
32808115	8	8	theme	mice	957:960	arg1	tissue					933:938	the epididymal adipose tissue	910:938	the epididymal adipose tissue of HFD-fed obese mice (HFD + F. plautii)	910:979	The oral administration of F. plautii attenuated the increase in TNF-α transcription otherwise seen in the epididymal adipose tissue of HFD-fed obese mice (HFD + F. plautii).
32808115	8	9	theme	obese	951:955	arg1	HFD + F.					963:970	HFD + F.	963:970	HFD + F.	963:970	The oral administration of F. plautii attenuated the increase in TNF-α transcription otherwise seen in the epididymal adipose tissue of HFD-fed obese mice (HFD + F. plautii).
32808115	8	9	theme	obese	951:955	arg1	mice					957:960	HFD-fed obese mice	943:960	HFD-fed obese mice (HFD + F. plautii)	943:979	The oral administration of F. plautii attenuated the increase in TNF-α transcription otherwise seen in the epididymal adipose tissue of HFD-fed obese mice (HFD + F. plautii).
32808115	9	10	theme	population	1015:1024	arg1	composition					986:996	The composition	982:996	The composition of the microbial population (at the genus level) in the cecal contents of the HFD + F. plautii mice	982:1096	The composition of the microbial population (at the genus level) in the cecal contents of the HFD + F. plautii mice was altered considerably.
32808115	2	11	from	present	214:220	arg1	feces					231:235	human feces	225:235	human feces	225:235	Flavonifractor plautii, a bacterium present in human feces, has been reported to participate in the metabolism of catechin in the gut.
32808115	2	12	theme	Flavonifractor	178:191	arg1	plautii					193:199	Flavonifractor plautii	178:199	Flavonifractor plautii	178:199	Flavonifractor plautii, a bacterium present in human feces, has been reported to participate in the metabolism of catechin in the gut.
32808115	2	12	theme	Flavonifractor	178:191	arg1	bacterium					204:212	a bacterium	202:212	a bacterium present in human feces	202:235	Flavonifractor plautii, a bacterium present in human feces, has been reported to participate in the metabolism of catechin in the gut.
32808115	8	13	theme	TNF-α	872:876	arg1	transcription					878:890	TNF-α transcription	872:890	TNF-α transcription	872:890	The oral administration of F. plautii attenuated the increase in TNF-α transcription otherwise seen in the epididymal adipose tissue of HFD-fed obese mice (HFD + F. plautii).
32808115	4	14	theme	plautii	413:419	arg1	function					398:405	the immunoregulatory function	377:405	the immunoregulatory function of F. plautii	377:419	We assessed the immunoregulatory function of F. plautii both in vitro and in vivo.
32808115	2	15	theme	catechin	292:299	arg1	metabolism					278:287	the metabolism	274:287	the metabolism of catechin in the gut	274:310	Flavonifractor plautii, a bacterium present in human feces, has been reported to participate in the metabolism of catechin in the gut.
32808115	9	16	theme	genus	1034:1038	arg1	level					1040:1044	the genus level	1030:1044	the genus level	1030:1044	The composition of the microbial population (at the genus level) in the cecal contents of the HFD + F. plautii mice was altered considerably.
32808115	3	17	theme	plautii	340:346	arg1	function					325:332	The precise function	313:332	The precise function of F. plautii	313:346	The precise function of F. plautii remains unclear.
32808115	4	18	theme	F.	410:411	arg1	plautii					413:419	F. plautii	410:419	F. plautii	410:419	We assessed the immunoregulatory function of F. plautii both in vitro and in vivo.
32808115	13	19	theme	adipose	1559:1565	arg1	tissue					1567:1572	adipose tissue	1559:1572	adipose tissue	1559:1572	Our results demonstrated that F. plautii may be useful for alleviating the inflammatory responses of adipose tissue.
32808115	8	20	dep	HFD + F.	963:970	arg1	plautii					972:978	HFD + F. plautii	963:978	HFD + F. plautii	963:978	The oral administration of F. plautii attenuated the increase in TNF-α transcription otherwise seen in the epididymal adipose tissue of HFD-fed obese mice (HFD + F. plautii).
32808115	5	21	theme	lipopolysaccharide-stimulated	556:584	arg1	cells					596:600	lipopolysaccharide-stimulated RAW 264.7 cells	556:600	lipopolysaccharide-stimulated RAW 264.7 cells	556:600	In vitro, we showed that both viable and heat-killed F. plautii attenuated TNF-α transcript accumulation in lipopolysaccharide-stimulated RAW 264.7 cells.
32808115	13	22	theme	tissue	1567:1572	arg1	responses					1546:1554	the inflammatory responses	1529:1554	the inflammatory responses of adipose tissue	1529:1572	Our results demonstrated that F. plautii may be useful for alleviating the inflammatory responses of adipose tissue.
32808115	5	23	theme	viable	478:483	arg1	plautii					504:510	both viable and heat-killed F. plautii	473:510	both viable and heat-killed F. plautii	473:510	In vitro, we showed that both viable and heat-killed F. plautii attenuated TNF-α transcript accumulation in lipopolysaccharide-stimulated RAW 264.7 cells.
32808115	2	24	from	feces	231:235	arg1	present					214:220	present	214:220	present	214:220	Flavonifractor plautii, a bacterium present in human feces, has been reported to participate in the metabolism of catechin in the gut.
32808115	6	25	theme	in	611:612	arg1	experiment					619:628	the in vivo experiment	607:628	the in vivo experiment	607:628	For the in vivo experiment, male C57BL/6 were placed on a high-fat diet (HFD) for 11 weeks.
32808115	5	26	theme	RAW	586:588	arg1	cells					596:600	lipopolysaccharide-stimulated RAW 264.7 cells	556:600	lipopolysaccharide-stimulated RAW 264.7 cells	556:600	In vitro, we showed that both viable and heat-killed F. plautii attenuated TNF-α transcript accumulation in lipopolysaccharide-stimulated RAW 264.7 cells.
32808115	8	27	theme	F.	834:835	arg1	plautii					837:843	F. plautii	834:843	F. plautii	834:843	The oral administration of F. plautii attenuated the increase in TNF-α transcription otherwise seen in the epididymal adipose tissue of HFD-fed obese mice (HFD + F. plautii).
32808115	2	28	attach	present	214:220	arg2	plautii					193:199	Flavonifractor plautii	178:199	Flavonifractor plautii	178:199	Flavonifractor plautii, a bacterium present in human feces, has been reported to participate in the metabolism of catechin in the gut.
32808115	2	28	attach	present	214:220	arg2	bacterium					204:212	a bacterium	202:212	a bacterium present in human feces	202:235	Flavonifractor plautii, a bacterium present in human feces, has been reported to participate in the metabolism of catechin in the gut.
32808115	2	28	attach	present	214:220	arg1	feces					231:235	human feces	225:235	human feces	225:235	Flavonifractor plautii, a bacterium present in human feces, has been reported to participate in the metabolism of catechin in the gut.
32808115	0	29	theme	Oral	0:3	arg1	administration					5:18	Oral administration	0:18	Oral administration of Flavonifractor plautii	0:44	Oral administration of Flavonifractor plautii attenuates inflammatory responses in obese adipose tissue.
32808115	11	30	theme	inflammation	1335:1346	arg1	development					1320:1330	the development	1316:1330	the development of inflammation in adipose tissue	1316:1364	Obesity is closely associated with the development of inflammation in adipose tissue.
32808115	12	31	theme	expression	1417:1426	arg1	inhibition					1397:1406	inhibition	1397:1406	inhibition of TNF-α expression in inflammatory environments	1397:1455	F. plautii may be involved in inhibition of TNF-α expression in inflammatory environments.
32808115	4	32	theme	immunoregulatory	381:396	arg1	function					398:405	the immunoregulatory function	377:405	the immunoregulatory function of F. plautii	377:419	We assessed the immunoregulatory function of F. plautii both in vitro and in vivo.
32808115	1	33	theme	Adipose	105:111	arg1	inflammation					120:131	Adipose tissue inflammation	105:131	Adipose tissue inflammation	105:131	Adipose tissue inflammation enhances the symptoms of metabolic syndrome.
32808115	7	34	theme	once-daily	783:792	arg1	gavage					799:804	once-daily oral gavage	783:804	once-daily oral gavage	783:804	During the final two weeks on the HFD, the animals were administered with F. plautii by once-daily oral gavage.
32808115	12	35	theme	inflammatory	1431:1442	arg1	environments					1444:1455	inflammatory environments	1431:1455	inflammatory environments	1431:1455	F. plautii may be involved in inhibition of TNF-α expression in inflammatory environments.
32808115	0	36	theme	Flavonifractor	23:36	arg1	plautii					38:44	Flavonifractor plautii	23:44	Flavonifractor plautii	23:44	Oral administration of Flavonifractor plautii attenuates inflammatory responses in obese adipose tissue.
32808115	7	37	theme	F.	769:770	arg1	plautii					772:778	F. plautii	769:778	F. plautii	769:778	During the final two weeks on the HFD, the animals were administered with F. plautii by once-daily oral gavage.
32808115	8	38	theme	HFD-fed	943:949	arg1	HFD + F.					963:970	HFD + F.	963:970	HFD + F.	963:970	The oral administration of F. plautii attenuated the increase in TNF-α transcription otherwise seen in the epididymal adipose tissue of HFD-fed obese mice (HFD + F. plautii).
32808115	8	38	theme	HFD-fed	943:949	arg1	mice					957:960	HFD-fed obese mice	943:960	HFD-fed obese mice (HFD + F. plautii)	943:979	The oral administration of F. plautii attenuated the increase in TNF-α transcription otherwise seen in the epididymal adipose tissue of HFD-fed obese mice (HFD + F. plautii).
32808115	1	39	theme	tissue	113:118	arg1	inflammation					120:131	Adipose tissue inflammation	105:131	Adipose tissue inflammation	105:131	Adipose tissue inflammation enhances the symptoms of metabolic syndrome.
32808115	7	40	theme	oral	794:797	arg1	gavage					799:804	once-daily oral gavage	783:804	once-daily oral gavage	783:804	During the final two weeks on the HFD, the animals were administered with F. plautii by once-daily oral gavage.
32808115	9	41	theme	plautii	1085:1091	arg1	mice					1093:1096	the HFD + F. plautii mice	1072:1096	the HFD + F. plautii mice	1072:1096	The composition of the microbial population (at the genus level) in the cecal contents of the HFD + F. plautii mice was altered considerably.
32808115	13	42	theme	inflammatory	1533:1544	arg1	responses					1546:1554	the inflammatory responses	1529:1554	the inflammatory responses of adipose tissue	1529:1572	Our results demonstrated that F. plautii may be useful for alleviating the inflammatory responses of adipose tissue.
32808115	6	43	theme	high-fat	661:668	arg1	diet					670:673	a high-fat diet	659:673	a high-fat diet (HFD) for 11 weeks	659:692	For the in vivo experiment, male C57BL/6 were placed on a high-fat diet (HFD) for 11 weeks.
32808115	6	43	theme	high-fat	661:668	arg1	HFD					676:678	HFD	676:678	HFD	676:678	For the in vivo experiment, male C57BL/6 were placed on a high-fat diet (HFD) for 11 weeks.
32808115	9	44	theme	microbial	1005:1013	arg1	population					1015:1024	the microbial population	1001:1024	the microbial population (at the genus level)	1001:1045	The composition of the microbial population (at the genus level) in the cecal contents of the HFD + F. plautii mice was altered considerably.
32808115	0	45	theme	plautii	38:44	arg1	administration					5:18	Oral administration	0:18	Oral administration of Flavonifractor plautii	0:44	Oral administration of Flavonifractor plautii attenuates inflammatory responses in obese adipose tissue.
32808115	9	46	from	composition	986:996	arg1	contents					1060:1067	the cecal contents	1050:1067	the cecal contents of the HFD + F. plautii mice	1050:1096	The composition of the microbial population (at the genus level) in the cecal contents of the HFD + F. plautii mice was altered considerably.
32808115	9	46	from	composition	986:996	arg1	level					1040:1044	the genus level	1030:1044	the genus level	1030:1044	The composition of the microbial population (at the genus level) in the cecal contents of the HFD + F. plautii mice was altered considerably.
32808115	9	47	theme	cecal	1054:1058	arg1	contents					1060:1067	the cecal contents	1050:1067	the cecal contents of the HFD + F. plautii mice	1050:1096	The composition of the microbial population (at the genus level) in the cecal contents of the HFD + F. plautii mice was altered considerably.
32808115	12	48	from	environments	1444:1455	arg1	inhibition					1397:1406	inhibition	1397:1406	inhibition of TNF-α expression in inflammatory environments	1397:1455	F. plautii may be involved in inhibition of TNF-α expression in inflammatory environments.
32808115	5	49	theme	TNF-α	523:527	arg1	accumulation					540:551	TNF-α transcript accumulation	523:551	TNF-α transcript accumulation	523:551	In vitro, we showed that both viable and heat-killed F. plautii attenuated TNF-α transcript accumulation in lipopolysaccharide-stimulated RAW 264.7 cells.
32808115	12	50	from	expression	1417:1426	arg1	environments					1444:1455	inflammatory environments	1431:1455	inflammatory environments	1431:1455	F. plautii may be involved in inhibition of TNF-α expression in inflammatory environments.
32808115	9	51	from	level	1040:1044	arg1	composition					986:996	The composition	982:996	The composition of the microbial population (at the genus level) in the cecal contents of the HFD + F. plautii mice	982:1096	The composition of the microbial population (at the genus level) in the cecal contents of the HFD + F. plautii mice was altered considerably.
32808115	9	51	from	level	1040:1044	arg1	population					1015:1024	the microbial population	1001:1024	the microbial population (at the genus level)	1001:1045	The composition of the microbial population (at the genus level) in the cecal contents of the HFD + F. plautii mice was altered considerably.
32808115	5	52	theme	transcript	529:538	arg1	accumulation					540:551	TNF-α transcript accumulation	523:551	TNF-α transcript accumulation	523:551	In vitro, we showed that both viable and heat-killed F. plautii attenuated TNF-α transcript accumulation in lipopolysaccharide-stimulated RAW 264.7 cells.
32808115	0	53	theme	inflammatory	57:68	arg1	responses					70:78	inflammatory responses	57:78	inflammatory responses	57:78	Oral administration of Flavonifractor plautii attenuates inflammatory responses in obese adipose tissue.
32808115	9	54	theme	HFD + F.	1076:1083	arg1	mice					1093:1096	the HFD + F. plautii mice	1072:1096	the HFD + F. plautii mice	1072:1096	The composition of the microbial population (at the genus level) in the cecal contents of the HFD + F. plautii mice was altered considerably.
32808115	7	55	from	weeks	716:720	arg1	HFD					729:731	the HFD	725:731	the HFD	725:731	During the final two weeks on the HFD, the animals were administered with F. plautii by once-daily oral gavage.
32808115	12	56	theme	TNF-α	1411:1415	arg1	expression					1417:1426	TNF-α expression	1411:1426	TNF-α expression in inflammatory environments	1411:1455	F. plautii may be involved in inhibition of TNF-α expression in inflammatory environments.
32808115	10	57	theme	plautii	1266:1272	arg1	group					1274:1278	the HFD + F. plautii group	1253:1278	the HFD + F. plautii group	1253:1278	In particular, the level of Sphingobium was decreased significantly, and that of Lachnospiraceae was increased significantly, in the HFD + F. plautii group.
32808115	9	58	theme	mice	1093:1096	arg1	contents					1060:1067	the cecal contents	1050:1067	the cecal contents of the HFD + F. plautii mice	1050:1096	The composition of the microbial population (at the genus level) in the cecal contents of the HFD + F. plautii mice was altered considerably.
32808115	6	59	dep	in	611:612	arg1	vivo					614:617	vivo	614:617	vivo	614:617	For the in vivo experiment, male C57BL/6 were placed on a high-fat diet (HFD) for 11 weeks.
32808115	2	60	theme	human	225:229	arg1	feces					231:235	human feces	225:235	human feces	225:235	Flavonifractor plautii, a bacterium present in human feces, has been reported to participate in the metabolism of catechin in the gut.
32808115	10	61	theme	HFD + F.	1257:1264	arg1	group					1274:1278	the HFD + F. plautii group	1253:1278	the HFD + F. plautii group	1253:1278	In particular, the level of Sphingobium was decreased significantly, and that of Lachnospiraceae was increased significantly, in the HFD + F. plautii group.
32808115	2	62	from	metabolism	278:287	arg1	gut					308:310	the gut	304:310	the gut	304:310	Flavonifractor plautii, a bacterium present in human feces, has been reported to participate in the metabolism of catechin in the gut.
32808115	11	63	from	development	1320:1330	arg1	tissue					1359:1364	adipose tissue	1351:1364	adipose tissue	1351:1364	Obesity is closely associated with the development of inflammation in adipose tissue.
32808115	8	64	from	increase	860:867	arg1	transcription					878:890	TNF-α transcription	872:890	TNF-α transcription	872:890	The oral administration of F. plautii attenuated the increase in TNF-α transcription otherwise seen in the epididymal adipose tissue of HFD-fed obese mice (HFD + F. plautii).
32808115	5	65	theme	heat-killed	489:499	arg1	plautii					504:510	both viable and heat-killed F. plautii	473:510	both viable and heat-killed F. plautii	473:510	In vitro, we showed that both viable and heat-killed F. plautii attenuated TNF-α transcript accumulation in lipopolysaccharide-stimulated RAW 264.7 cells.
32808115	10	66	theme	Sphingobium	1152:1162	arg1	level					1143:1147	the level	1139:1147	the level of Sphingobium	1139:1162	In particular, the level of Sphingobium was decreased significantly, and that of Lachnospiraceae was increased significantly, in the HFD + F. plautii group.
32808115	8	67	theme	plautii	837:843	arg1	administration					816:829	The oral administration	807:829	The oral administration of F. plautii	807:843	The oral administration of F. plautii attenuated the increase in TNF-α transcription otherwise seen in the epididymal adipose tissue of HFD-fed obese mice (HFD + F. plautii).
32808115	0	68	theme	obese	83:87	arg1	tissue					97:102	obese adipose tissue	83:102	obese adipose tissue	83:102	Oral administration of Flavonifractor plautii attenuates inflammatory responses in obese adipose tissue.
32808115	2	69	theme	present	214:220	arg1	plautii					193:199	Flavonifractor plautii	178:199	Flavonifractor plautii	178:199	Flavonifractor plautii, a bacterium present in human feces, has been reported to participate in the metabolism of catechin in the gut.
32808115	2	69	theme	present	214:220	arg1	bacterium					204:212	a bacterium	202:212	a bacterium present in human feces	202:235	Flavonifractor plautii, a bacterium present in human feces, has been reported to participate in the metabolism of catechin in the gut.
32808115	5	70	theme	F.	501:502	arg1	plautii					504:510	both viable and heat-killed F. plautii	473:510	both viable and heat-killed F. plautii	473:510	In vitro, we showed that both viable and heat-killed F. plautii attenuated TNF-α transcript accumulation in lipopolysaccharide-stimulated RAW 264.7 cells.
32808115	1	71	theme	metabolic	158:166	arg1	syndrome					168:175	metabolic syndrome	158:175	metabolic syndrome	158:175	Adipose tissue inflammation enhances the symptoms of metabolic syndrome.
32808115	3	72	theme	F.	337:338	arg1	plautii					340:346	F. plautii	337:346	F. plautii	337:346	The precise function of F. plautii remains unclear.
32808115	13	73	theme	F.	1488:1489	arg1	plautii					1491:1497	F. plautii	1488:1497	F. plautii	1488:1497	Our results demonstrated that F. plautii may be useful for alleviating the inflammatory responses of adipose tissue.
32808115	8	74	theme	adipose	925:931	arg1	tissue					933:938	the epididymal adipose tissue	910:938	the epididymal adipose tissue of HFD-fed obese mice (HFD + F. plautii)	910:979	The oral administration of F. plautii attenuated the increase in TNF-α transcription otherwise seen in the epididymal adipose tissue of HFD-fed obese mice (HFD + F. plautii).
32808115	1	75	theme	syndrome	168:175	arg1	symptoms					146:153	the symptoms	142:153	the symptoms of metabolic syndrome	142:175	Adipose tissue inflammation enhances the symptoms of metabolic syndrome.
32808115	6	76	theme	male	631:634	arg1	C57BL/6					636:642	male C57BL/6	631:642	male C57BL/6	631:642	For the in vivo experiment, male C57BL/6 were placed on a high-fat diet (HFD) for 11 weeks.
34015001	6	0	theme	sugar	1240:1244	arg1	addition					1246:1253	an N-linked but not O-linked sugar addition	1211:1253	an N-linked but not O-linked sugar addition	1211:1253	Additional analysis suggests that mutations of the potential WAK EGF O-fucosylation site also abate the WAK2cTAP phenotype, yet only evidence for an N-linked but not O-linked sugar addition can be found.
34015001	2	1	theme	putative	349:356	arg1	ESMD1					391:395	ESMD1	391:395	ESMD1	391:395	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	2	1	theme	putative	349:356	arg1	ESMERALDA1					379:388	a putative O-fucosyltransferase ESMERALDA1	347:388	a putative O-fucosyltransferase ESMERALDA1 (ESMD1)	347:396	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	8	2	theme	transcriptional	1610:1624	arg1	response					1626:1633	the transcriptional response	1606:1633	the induction of ROS but not the transcriptional response induced by the elicitors Flagellin, Chitin and oligogalacturonides (OGs)	1577:1706	The WAK locus deletion does however affect the induction of ROS but not the transcriptional response induced by the elicitors Flagellin, Chitin and oligogalacturonides (OGs).
34015001	7	3	theme	adhesion	1360:1367	arg1	deficiency					1369:1378	an adhesion deficiency	1357:1378	an adhesion deficiency	1357:1378	Moreover, a WAK locus deletion allele has no effect on the ability of esmd1 to suppress an adhesion deficiency, indicating WAKs and their modification are not a required component of the potential ESMD1 signaling mechanism involved in the control of cell adhesion.
34015001	3	4	theme	putative	685:692	arg1	ESMD1					715:719	the putative O-fucosyltransferase ESMD1	681:719	the putative O-fucosyltransferase ESMD1	681:719	Genetic interactions between WAKs and ESMD1 were examined using a dominant hyperactive allele of WAK2, WAK2cTAP, and a mutant of the putative O-fucosyltransferase ESMD1.
34015001	4	5	theme	oxygen	822:827	arg1	species					829:835	reactive oxygen species	813:835	reactive oxygen species (ROS) production	813:852	WAK2cTAP expression results in a dwarf phenotype and activation of the stress response and reactive oxygen species (ROS) production, while esmd1 is a suppressor of a pectin deficiency induced loss of adhesion.
34015001	4	5	theme	oxygen	822:827	arg1	ROS					838:840	ROS	838:840	ROS	838:840	WAK2cTAP expression results in a dwarf phenotype and activation of the stress response and reactive oxygen species (ROS) production, while esmd1 is a suppressor of a pectin deficiency induced loss of adhesion.
34015001	6	6	theme	O-fucosylation	1134:1147	arg1	site					1149:1152	the potential WAK EGF O-fucosylation site	1112:1152	the potential WAK EGF O-fucosylation site	1112:1152	Additional analysis suggests that mutations of the potential WAK EGF O-fucosylation site also abate the WAK2cTAP phenotype, yet only evidence for an N-linked but not O-linked sugar addition can be found.
34015001	6	7	theme	N-linked	1214:1221	arg1	addition					1246:1253	an N-linked but not O-linked sugar addition	1211:1253	an N-linked but not O-linked sugar addition	1211:1253	Additional analysis suggests that mutations of the potential WAK EGF O-fucosylation site also abate the WAK2cTAP phenotype, yet only evidence for an N-linked but not O-linked sugar addition can be found.
34015001	7	8	theme	esmd1	1339:1343	arg1	ability					1328:1334	the ability	1324:1334	the ability of esmd1 to suppress an adhesion deficiency	1324:1378	Moreover, a WAK locus deletion allele has no effect on the ability of esmd1 to suppress an adhesion deficiency, indicating WAKs and their modification are not a required component of the potential ESMD1 signaling mechanism involved in the control of cell adhesion.
34015001	2	9	theme	pectin-related	302:315	arg1	cascade					327:333	a pectin-related signaling cascade	300:333	a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1)	300:396	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	3	10	theme	ESMD1	715:719	arg1	WAK2					649:652	WAK2	649:652	WAK2	649:652	Genetic interactions between WAKs and ESMD1 were examined using a dominant hyperactive allele of WAK2, WAK2cTAP, and a mutant of the putative O-fucosyltransferase ESMD1.
34015001	3	10	theme	ESMD1	715:719	arg1	mutant					671:676	a mutant	669:676	a mutant of the putative O-fucosyltransferase ESMD1	669:719	Genetic interactions between WAKs and ESMD1 were examined using a dominant hyperactive allele of WAK2, WAK2cTAP, and a mutant of the putative O-fucosyltransferase ESMD1.
34015001	3	10	theme	ESMD1	715:719	arg1	WAK2cTAP					655:662	WAK2cTAP	655:662	WAK2cTAP	655:662	Genetic interactions between WAKs and ESMD1 were examined using a dominant hyperactive allele of WAK2, WAK2cTAP, and a mutant of the putative O-fucosyltransferase ESMD1.
34015001	4	11	theme	pectin	888:893	arg1	deficiency					895:904	a pectin deficiency	886:904	a pectin deficiency induced loss of adhesion	886:929	WAK2cTAP expression results in a dwarf phenotype and activation of the stress response and reactive oxygen species (ROS) production, while esmd1 is a suppressor of a pectin deficiency induced loss of adhesion.
34015001	6	12	link	N-linked	1214:1221	arg1	addition					1246:1253	an N-linked but not O-linked sugar addition	1211:1253	an N-linked but not O-linked sugar addition	1211:1253	Additional analysis suggests that mutations of the potential WAK EGF O-fucosylation site also abate the WAK2cTAP phenotype, yet only evidence for an N-linked but not O-linked sugar addition can be found.
34015001	6	13	gly	O-fucosylation	1134:1147	arg2	site					1149:1152	the potential WAK EGF O-fucosylation site	1112:1152	the potential WAK EGF O-fucosylation site	1112:1152	Additional analysis suggests that mutations of the potential WAK EGF O-fucosylation site also abate the WAK2cTAP phenotype, yet only evidence for an N-linked but not O-linked sugar addition can be found.
34015001	5	14	theme	stress	990:995	arg1	phenotype					1006:1014	the WAK2cTAP dwarf and stress response phenotype	967:1014	phenotype	1006:1014	Here we find that esmd1 suppresses the WAK2cTAP dwarf and stress response phenotype, including ROS accumulation and gene expression.
34015001	2	15	theme	Kinases	483:489	arg1	substrate					522:530	a primary candidate substrate	502:530	a primary candidate substrate for ESMD1 activity	502:549	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	2	15	theme	Kinases	483:489	arg1	Kinases					483:489	the pectin binding Wall associated Kinases	448:489	the pectin binding Wall associated Kinases (WAKs)	448:496	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	2	15	theme	Kinases	483:489	arg1	domains					437:443	the Epidermal Growth Factor (EGF) domains	403:443	the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs)	403:496	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	7	16	theme	adhesion	1524:1531	arg1	control					1508:1514	the control	1504:1514	the control of cell adhesion	1504:1531	Moreover, a WAK locus deletion allele has no effect on the ability of esmd1 to suppress an adhesion deficiency, indicating WAKs and their modification are not a required component of the potential ESMD1 signaling mechanism involved in the control of cell adhesion.
34015001	7	17	theme	locus	1285:1289	arg1	allele					1300:1305	a WAK locus deletion allele	1279:1305	a WAK locus deletion allele	1279:1305	Moreover, a WAK locus deletion allele has no effect on the ability of esmd1 to suppress an adhesion deficiency, indicating WAKs and their modification are not a required component of the potential ESMD1 signaling mechanism involved in the control of cell adhesion.
34015001	2	18	theme	Cell	241:244	arg1	adhesion					246:253	Cell adhesion	241:253	Cell adhesion in Arabidopsis	241:268	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	2	19	theme	associated	472:481	arg1	Kinases					483:489	the pectin binding Wall associated Kinases	448:489	the pectin binding Wall associated Kinases (WAKs)	448:496	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	2	19	theme	associated	472:481	arg1	WAKs					492:495	WAKs	492:495	WAKs	492:495	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	0	20	from	Effects	0:6	arg1	elicitor					74:81	elicitor	74:81	elicitor	74:81	Effects of Arabidopsis wall associated kinase mutations on ESMERALDA1 and elicitor induced ROS.
34015001	0	20	from	Effects	0:6	arg1	ESMERALDA1					59:68	ESMERALDA1	59:68	ESMERALDA1	59:68	Effects of Arabidopsis wall associated kinase mutations on ESMERALDA1 and elicitor induced ROS.
34015001	5	21	theme	gene	1048:1051	arg1	expression					1053:1062	gene expression	1048:1062	gene expression	1048:1062	Here we find that esmd1 suppresses the WAK2cTAP dwarf and stress response phenotype, including ROS accumulation and gene expression.
34015001	8	22	dep	elicitors	1650:1658	arg1	Flagellin					1660:1668	Flagellin	1660:1668	Flagellin	1660:1668	The WAK locus deletion does however affect the induction of ROS but not the transcriptional response induced by the elicitors Flagellin, Chitin and oligogalacturonides (OGs).
34015001	8	22	dep	elicitors	1650:1658	arg1	OGs					1703:1705	OGs	1703:1705	OGs	1703:1705	The WAK locus deletion does however affect the induction of ROS but not the transcriptional response induced by the elicitors Flagellin, Chitin and oligogalacturonides (OGs).
34015001	8	22	dep	elicitors	1650:1658	arg1	oligogalacturonides					1682:1700	oligogalacturonides	1682:1700	oligogalacturonides (OGs)	1682:1706	The WAK locus deletion does however affect the induction of ROS but not the transcriptional response induced by the elicitors Flagellin, Chitin and oligogalacturonides (OGs).
34015001	8	22	dep	elicitors	1650:1658	arg1	elicitors					1650:1658	the elicitors Flagellin, Chitin and oligogalacturonides (OGs)	1646:1706	the elicitors Flagellin, Chitin and oligogalacturonides (OGs)	1646:1706	The WAK locus deletion does however affect the induction of ROS but not the transcriptional response induced by the elicitors Flagellin, Chitin and oligogalacturonides (OGs).
34015001	8	22	dep	elicitors	1650:1658	arg1	Chitin					1671:1676	Chitin	1671:1676	Chitin	1671:1676	The WAK locus deletion does however affect the induction of ROS but not the transcriptional response induced by the elicitors Flagellin, Chitin and oligogalacturonides (OGs).
34015001	4	23	theme	dwarf	755:759	arg1	phenotype					761:769	a dwarf phenotype	753:769	a dwarf phenotype	753:769	WAK2cTAP expression results in a dwarf phenotype and activation of the stress response and reactive oxygen species (ROS) production, while esmd1 is a suppressor of a pectin deficiency induced loss of adhesion.
34015001	7	24	theme	WAK	1281:1283	arg1	allele					1300:1305	a WAK locus deletion allele	1279:1305	a WAK locus deletion allele	1279:1305	Moreover, a WAK locus deletion allele has no effect on the ability of esmd1 to suppress an adhesion deficiency, indicating WAKs and their modification are not a required component of the potential ESMD1 signaling mechanism involved in the control of cell adhesion.
34015001	2	25	from	adhesion	246:253	arg1	Arabidopsis					258:268	Arabidopsis	258:268	Arabidopsis	258:268	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	2	26	theme	Factor	424:429	arg1	substrate					522:530	a primary candidate substrate	502:530	a primary candidate substrate for ESMD1 activity	502:549	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	2	26	theme	Factor	424:429	arg1	Kinases					483:489	the pectin binding Wall associated Kinases	448:489	the pectin binding Wall associated Kinases (WAKs)	448:496	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	2	26	theme	Factor	424:429	arg1	domains					437:443	the Epidermal Growth Factor (EGF) domains	403:443	the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs)	403:496	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	0	27	theme	Arabidopsis	11:21	arg1	mutations					46:54	Arabidopsis wall associated kinase mutations	11:54	Arabidopsis wall associated kinase mutations	11:54	Effects of Arabidopsis wall associated kinase mutations on ESMERALDA1 and elicitor induced ROS.
34015001	7	28	theme	ESMD1	1466:1470	arg1	mechanism					1482:1490	the potential ESMD1 signaling mechanism	1452:1490	the potential ESMD1 signaling mechanism involved in the control of cell adhesion	1452:1531	Moreover, a WAK locus deletion allele has no effect on the ability of esmd1 to suppress an adhesion deficiency, indicating WAKs and their modification are not a required component of the potential ESMD1 signaling mechanism involved in the control of cell adhesion.
34015001	1	29	theme	cell	107:110	arg1	adhesion					112:119	Angiosperm cell adhesion	96:119	Angiosperm cell adhesion	96:119	Angiosperm cell adhesion is dependent on interactions between pectin polysaccharides which make up a significant portion of the plant cell wall.
34015001	2	30	theme	binding	459:465	arg1	Wall					467:470	the pectin binding Wall	448:470	the pectin binding Wall associated Kinases (WAKs)	448:496	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	4	31	theme	stress	793:798	arg1	response					800:807	the stress response	789:807	the stress response	789:807	WAK2cTAP expression results in a dwarf phenotype and activation of the stress response and reactive oxygen species (ROS) production, while esmd1 is a suppressor of a pectin deficiency induced loss of adhesion.
34015001	2	32	theme	Epidermal	407:415	arg1	EGF					432:434	EGF	432:434	EGF	432:434	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	2	32	theme	Epidermal	407:415	arg1	Factor					424:429	the Epidermal Growth Factor	403:429	the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs)	403:496	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	0	33	theme	associated	28:37	arg1	mutations					46:54	Arabidopsis wall associated kinase mutations	11:54	Arabidopsis wall associated kinase mutations	11:54	Effects of Arabidopsis wall associated kinase mutations on ESMERALDA1 and elicitor induced ROS.
34015001	5	34	theme	WAK2cTAP	971:978	arg1	dwarf					980:984	the WAK2cTAP dwarf and stress response phenotype	967:1014	dwarf	980:984	Here we find that esmd1 suppresses the WAK2cTAP dwarf and stress response phenotype, including ROS accumulation and gene expression.
34015001	3	35	theme	dominant	618:625	arg1	allele					639:644	a dominant hyperactive allele	616:644	a dominant hyperactive allele of WAK2, WAK2cTAP, and a mutant of the putative O-fucosyltransferase ESMD1	616:719	Genetic interactions between WAKs and ESMD1 were examined using a dominant hyperactive allele of WAK2, WAK2cTAP, and a mutant of the putative O-fucosyltransferase ESMD1.
34015001	0	36	theme	mutations	46:54	arg1	Effects					0:6	Effects	0:6	Effects of Arabidopsis wall associated kinase mutations on ESMERALDA1 and elicitor	0:81	Effects of Arabidopsis wall associated kinase mutations on ESMERALDA1 and elicitor induced ROS.
34015001	3	37	theme	WAK2cTAP	655:662	arg1	allele					639:644	a dominant hyperactive allele	616:644	a dominant hyperactive allele of WAK2, WAK2cTAP, and a mutant of the putative O-fucosyltransferase ESMD1	616:719	Genetic interactions between WAKs and ESMD1 were examined using a dominant hyperactive allele of WAK2, WAK2cTAP, and a mutant of the putative O-fucosyltransferase ESMD1.
34015001	1	38	theme	plant	224:228	arg1	wall					235:238	the plant cell wall	220:238	the plant cell wall	220:238	Angiosperm cell adhesion is dependent on interactions between pectin polysaccharides which make up a significant portion of the plant cell wall.
34015001	3	39	theme	WAK2	649:652	arg1	allele					639:644	a dominant hyperactive allele	616:644	a dominant hyperactive allele of WAK2, WAK2cTAP, and a mutant of the putative O-fucosyltransferase ESMD1	616:719	Genetic interactions between WAKs and ESMD1 were examined using a dominant hyperactive allele of WAK2, WAK2cTAP, and a mutant of the putative O-fucosyltransferase ESMD1.
34015001	7	40	contain	has	1307:1309	arg1	allele					1300:1305	a WAK locus deletion allele	1279:1305	a WAK locus deletion allele	1279:1305	Moreover, a WAK locus deletion allele has no effect on the ability of esmd1 to suppress an adhesion deficiency, indicating WAKs and their modification are not a required component of the potential ESMD1 signaling mechanism involved in the control of cell adhesion.
34015001	7	40	contain	has	1307:1309	arg2	effect					1314:1319	no effect	1311:1319	no effect	1311:1319	Moreover, a WAK locus deletion allele has no effect on the ability of esmd1 to suppress an adhesion deficiency, indicating WAKs and their modification are not a required component of the potential ESMD1 signaling mechanism involved in the control of cell adhesion.
34015001	8	41	theme	WAK	1538:1540	arg1	deletion					1548:1555	The WAK locus deletion	1534:1555	The WAK locus deletion	1534:1555	The WAK locus deletion does however affect the induction of ROS but not the transcriptional response induced by the elicitors Flagellin, Chitin and oligogalacturonides (OGs).
34015001	3	42	theme	mutant	671:676	arg1	allele					639:644	a dominant hyperactive allele	616:644	a dominant hyperactive allele of WAK2, WAK2cTAP, and a mutant of the putative O-fucosyltransferase ESMD1	616:719	Genetic interactions between WAKs and ESMD1 were examined using a dominant hyperactive allele of WAK2, WAK2cTAP, and a mutant of the putative O-fucosyltransferase ESMD1.
34015001	2	43	theme	O-fucosyltransferase	358:377	arg1	ESMD1					391:395	ESMD1	391:395	ESMD1	391:395	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	2	43	theme	O-fucosyltransferase	358:377	arg1	ESMERALDA1					379:388	a putative O-fucosyltransferase ESMERALDA1	347:388	a putative O-fucosyltransferase ESMERALDA1 (ESMD1)	347:396	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	6	44	theme	O-linked	1231:1238	arg1	addition					1246:1253	an N-linked but not O-linked sugar addition	1211:1253	an N-linked but not O-linked sugar addition	1211:1253	Additional analysis suggests that mutations of the potential WAK EGF O-fucosylation site also abate the WAK2cTAP phenotype, yet only evidence for an N-linked but not O-linked sugar addition can be found.
34015001	6	45	theme	WAK	1126:1128	arg1	site					1149:1152	the potential WAK EGF O-fucosylation site	1112:1152	the potential WAK EGF O-fucosylation site	1112:1152	Additional analysis suggests that mutations of the potential WAK EGF O-fucosylation site also abate the WAK2cTAP phenotype, yet only evidence for an N-linked but not O-linked sugar addition can be found.
34015001	6	46	theme	site	1149:1152	arg1	mutations					1099:1107	mutations	1099:1107	mutations of the potential WAK EGF O-fucosylation site	1099:1152	Additional analysis suggests that mutations of the potential WAK EGF O-fucosylation site also abate the WAK2cTAP phenotype, yet only evidence for an N-linked but not O-linked sugar addition can be found.
34015001	6	47	link	O-linked	1231:1238	arg1	addition					1246:1253	an N-linked but not O-linked sugar addition	1211:1253	an N-linked but not O-linked sugar addition	1211:1253	Additional analysis suggests that mutations of the potential WAK EGF O-fucosylation site also abate the WAK2cTAP phenotype, yet only evidence for an N-linked but not O-linked sugar addition can be found.
34015001	3	48	theme	O-fucosyltransferase	694:713	arg1	ESMD1					715:719	the putative O-fucosyltransferase ESMD1	681:719	the putative O-fucosyltransferase ESMD1	681:719	Genetic interactions between WAKs and ESMD1 were examined using a dominant hyperactive allele of WAK2, WAK2cTAP, and a mutant of the putative O-fucosyltransferase ESMD1.
34015001	4	49	theme	reactive	813:820	arg1	species					829:835	reactive oxygen species	813:835	reactive oxygen species (ROS) production	813:852	WAK2cTAP expression results in a dwarf phenotype and activation of the stress response and reactive oxygen species (ROS) production, while esmd1 is a suppressor of a pectin deficiency induced loss of adhesion.
34015001	4	49	theme	reactive	813:820	arg1	ROS					838:840	ROS	838:840	ROS	838:840	WAK2cTAP expression results in a dwarf phenotype and activation of the stress response and reactive oxygen species (ROS) production, while esmd1 is a suppressor of a pectin deficiency induced loss of adhesion.
34015001	6	50	theme	EGF	1130:1132	arg1	site					1149:1152	the potential WAK EGF O-fucosylation site	1112:1152	the potential WAK EGF O-fucosylation site	1112:1152	Additional analysis suggests that mutations of the potential WAK EGF O-fucosylation site also abate the WAK2cTAP phenotype, yet only evidence for an N-linked but not O-linked sugar addition can be found.
34015001	2	51	theme	signaling	317:325	arg1	cascade					327:333	a pectin-related signaling cascade	300:333	a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1)	300:396	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	4	52	theme	species	829:835	arg1	production					843:852	reactive oxygen species (ROS) production	813:852	reactive oxygen species (ROS) production	813:852	WAK2cTAP expression results in a dwarf phenotype and activation of the stress response and reactive oxygen species (ROS) production, while esmd1 is a suppressor of a pectin deficiency induced loss of adhesion.
34015001	6	53	theme	potential	1116:1124	arg1	site					1149:1152	the potential WAK EGF O-fucosylation site	1112:1152	the potential WAK EGF O-fucosylation site	1112:1152	Additional analysis suggests that mutations of the potential WAK EGF O-fucosylation site also abate the WAK2cTAP phenotype, yet only evidence for an N-linked but not O-linked sugar addition can be found.
34015001	1	54	theme	significant	197:207	arg1	portion					209:215	a significant portion	195:215	a significant portion of the plant cell wall	195:238	Angiosperm cell adhesion is dependent on interactions between pectin polysaccharides which make up a significant portion of the plant cell wall.
34015001	3	55	theme	Genetic	552:558	arg1	interactions					560:571	Genetic interactions	552:571	Genetic interactions between WAKs and ESMD1	552:594	Genetic interactions between WAKs and ESMD1 were examined using a dominant hyperactive allele of WAK2, WAK2cTAP, and a mutant of the putative O-fucosyltransferase ESMD1.
34015001	5	56	theme	ROS	1027:1029	arg1	accumulation					1031:1042	ROS accumulation	1027:1042	ROS accumulation	1027:1042	Here we find that esmd1 suppresses the WAK2cTAP dwarf and stress response phenotype, including ROS accumulation and gene expression.
34015001	2	57	theme	primary	504:510	arg1	substrate					522:530	a primary candidate substrate	502:530	a primary candidate substrate for ESMD1 activity	502:549	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	2	57	theme	primary	504:510	arg1	Kinases					483:489	the pectin binding Wall associated Kinases	448:489	the pectin binding Wall associated Kinases (WAKs)	448:496	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	2	57	theme	primary	504:510	arg1	domains					437:443	the Epidermal Growth Factor (EGF) domains	403:443	the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs)	403:496	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	6	58	theme	only	1193:1196	arg1	evidence					1198:1205	only evidence	1193:1205	only evidence for an N-linked but not O-linked sugar addition	1193:1253	Additional analysis suggests that mutations of the potential WAK EGF O-fucosylation site also abate the WAK2cTAP phenotype, yet only evidence for an N-linked but not O-linked sugar addition can be found.
34015001	5	59	theme	response	997:1004	arg1	phenotype					1006:1014	the WAK2cTAP dwarf and stress response phenotype	967:1014	phenotype	1006:1014	Here we find that esmd1 suppresses the WAK2cTAP dwarf and stress response phenotype, including ROS accumulation and gene expression.
34015001	7	60	theme	cell	1519:1522	arg1	adhesion					1524:1531	cell adhesion	1519:1531	cell adhesion	1519:1531	Moreover, a WAK locus deletion allele has no effect on the ability of esmd1 to suppress an adhesion deficiency, indicating WAKs and their modification are not a required component of the potential ESMD1 signaling mechanism involved in the control of cell adhesion.
34015001	2	61	theme	ESMD1	536:540	arg1	activity					542:549	ESMD1 activity	536:549	ESMD1 activity	536:549	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	6	62	theme	WAK2cTAP	1169:1176	arg1	phenotype					1178:1186	the WAK2cTAP phenotype	1165:1186	the WAK2cTAP phenotype	1165:1186	Additional analysis suggests that mutations of the potential WAK EGF O-fucosylation site also abate the WAK2cTAP phenotype, yet only evidence for an N-linked but not O-linked sugar addition can be found.
34015001	4	63	theme	adhesion	922:929	arg1	loss					914:917	loss	914:917	loss of adhesion	914:929	WAK2cTAP expression results in a dwarf phenotype and activation of the stress response and reactive oxygen species (ROS) production, while esmd1 is a suppressor of a pectin deficiency induced loss of adhesion.
34015001	7	64	theme	deletion	1291:1298	arg1	allele					1300:1305	a WAK locus deletion allele	1279:1305	a WAK locus deletion allele	1279:1305	Moreover, a WAK locus deletion allele has no effect on the ability of esmd1 to suppress an adhesion deficiency, indicating WAKs and their modification are not a required component of the potential ESMD1 signaling mechanism involved in the control of cell adhesion.
34015001	1	65	theme	pectin	158:163	arg1	polysaccharides					165:179	pectin polysaccharides	158:179	pectin polysaccharides which make up a significant portion of the plant cell wall	158:238	Angiosperm cell adhesion is dependent on interactions between pectin polysaccharides which make up a significant portion of the plant cell wall.
34015001	2	66	theme	Wall	467:470	arg1	Kinases					483:489	the pectin binding Wall associated Kinases	448:489	the pectin binding Wall associated Kinases (WAKs)	448:496	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	2	66	theme	Wall	467:470	arg1	WAKs					492:495	WAKs	492:495	WAKs	492:495	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	7	67	theme	mechanism	1482:1490	arg1	component					1439:1447	a required component	1428:1447	a required component of the potential ESMD1 signaling mechanism involved in the control of cell adhesion	1428:1531	Moreover, a WAK locus deletion allele has no effect on the ability of esmd1 to suppress an adhesion deficiency, indicating WAKs and their modification are not a required component of the potential ESMD1 signaling mechanism involved in the control of cell adhesion.
34015001	7	67	theme	mechanism	1482:1490	arg1	WAKs					1392:1395	WAKs	1392:1395	WAKs	1392:1395	Moreover, a WAK locus deletion allele has no effect on the ability of esmd1 to suppress an adhesion deficiency, indicating WAKs and their modification are not a required component of the potential ESMD1 signaling mechanism involved in the control of cell adhesion.
34015001	7	67	theme	mechanism	1482:1490	arg1	modification					1407:1418	their modification	1401:1418	their modification	1401:1418	Moreover, a WAK locus deletion allele has no effect on the ability of esmd1 to suppress an adhesion deficiency, indicating WAKs and their modification are not a required component of the potential ESMD1 signaling mechanism involved in the control of cell adhesion.
34015001	4	68	theme	deficiency	895:904	arg1	esmd1					861:865	esmd1	861:865	esmd1	861:865	WAK2cTAP expression results in a dwarf phenotype and activation of the stress response and reactive oxygen species (ROS) production, while esmd1 is a suppressor of a pectin deficiency induced loss of adhesion.
34015001	4	68	theme	deficiency	895:904	arg1	suppressor					872:881	a suppressor	870:881	a suppressor of a pectin deficiency induced loss of adhesion	870:929	WAK2cTAP expression results in a dwarf phenotype and activation of the stress response and reactive oxygen species (ROS) production, while esmd1 is a suppressor of a pectin deficiency induced loss of adhesion.
34015001	2	69	theme	candidate	512:520	arg1	substrate					522:530	a primary candidate substrate	502:530	a primary candidate substrate for ESMD1 activity	502:549	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	2	69	theme	candidate	512:520	arg1	Kinases					483:489	the pectin binding Wall associated Kinases	448:489	the pectin binding Wall associated Kinases (WAKs)	448:496	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	2	69	theme	candidate	512:520	arg1	domains					437:443	the Epidermal Growth Factor (EGF) domains	403:443	the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs)	403:496	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	0	70	theme	wall	23:26	arg1	mutations					46:54	Arabidopsis wall associated kinase mutations	11:54	Arabidopsis wall associated kinase mutations	11:54	Effects of Arabidopsis wall associated kinase mutations on ESMERALDA1 and elicitor induced ROS.
34015001	1	71	theme	Angiosperm	96:105	arg1	adhesion					112:119	Angiosperm cell adhesion	96:119	Angiosperm cell adhesion	96:119	Angiosperm cell adhesion is dependent on interactions between pectin polysaccharides which make up a significant portion of the plant cell wall.
34015001	3	72	theme	hyperactive	627:637	arg1	allele					639:644	a dominant hyperactive allele	616:644	a dominant hyperactive allele of WAK2, WAK2cTAP, and a mutant of the putative O-fucosyltransferase ESMD1	616:719	Genetic interactions between WAKs and ESMD1 were examined using a dominant hyperactive allele of WAK2, WAK2cTAP, and a mutant of the putative O-fucosyltransferase ESMD1.
34015001	4	73	theme	WAK2cTAP	722:729	arg1	expression					731:740	WAK2cTAP expression	722:740	WAK2cTAP expression	722:740	WAK2cTAP expression results in a dwarf phenotype and activation of the stress response and reactive oxygen species (ROS) production, while esmd1 is a suppressor of a pectin deficiency induced loss of adhesion.
34015001	2	74	theme	Growth	417:422	arg1	EGF					432:434	EGF	432:434	EGF	432:434	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	2	74	theme	Growth	417:422	arg1	Factor					424:429	the Epidermal Growth Factor	403:429	the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs)	403:496	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	0	75	theme	kinase	39:44	arg1	mutations					46:54	Arabidopsis wall associated kinase mutations	11:54	Arabidopsis wall associated kinase mutations	11:54	Effects of Arabidopsis wall associated kinase mutations on ESMERALDA1 and elicitor induced ROS.
34015001	7	76	theme	signaling	1472:1480	arg1	mechanism					1482:1490	the potential ESMD1 signaling mechanism	1452:1490	the potential ESMD1 signaling mechanism involved in the control of cell adhesion	1452:1531	Moreover, a WAK locus deletion allele has no effect on the ability of esmd1 to suppress an adhesion deficiency, indicating WAKs and their modification are not a required component of the potential ESMD1 signaling mechanism involved in the control of cell adhesion.
34015001	2	77	theme	pectin	452:457	arg1	Wall					467:470	the pectin binding Wall	448:470	the pectin binding Wall associated Kinases (WAKs)	448:496	Cell adhesion in Arabidopsis may also be regulated through a pectin-related signaling cascade mediated by a putative O-fucosyltransferase ESMERALDA1 (ESMD1), and the Epidermal Growth Factor (EGF) domains of the pectin binding Wall associated Kinases (WAKs) are a primary candidate substrate for ESMD1 activity.
34015001	1	78	theme	wall	235:238	arg1	portion					209:215	a significant portion	195:215	a significant portion of the plant cell wall	195:238	Angiosperm cell adhesion is dependent on interactions between pectin polysaccharides which make up a significant portion of the plant cell wall.
34015001	7	79	theme	potential	1456:1464	arg1	mechanism					1482:1490	the potential ESMD1 signaling mechanism	1452:1490	the potential ESMD1 signaling mechanism involved in the control of cell adhesion	1452:1531	Moreover, a WAK locus deletion allele has no effect on the ability of esmd1 to suppress an adhesion deficiency, indicating WAKs and their modification are not a required component of the potential ESMD1 signaling mechanism involved in the control of cell adhesion.
34015001	4	80	theme	response	800:807	arg1	activation					775:784	activation	775:784	activation of the stress response	775:807	WAK2cTAP expression results in a dwarf phenotype and activation of the stress response and reactive oxygen species (ROS) production, while esmd1 is a suppressor of a pectin deficiency induced loss of adhesion.
34015001	4	80	theme	response	800:807	arg1	phenotype					761:769	a dwarf phenotype	753:769	a dwarf phenotype	753:769	WAK2cTAP expression results in a dwarf phenotype and activation of the stress response and reactive oxygen species (ROS) production, while esmd1 is a suppressor of a pectin deficiency induced loss of adhesion.
34015001	4	80	theme	response	800:807	arg1	production					843:852	reactive oxygen species (ROS) production	813:852	reactive oxygen species (ROS) production	813:852	WAK2cTAP expression results in a dwarf phenotype and activation of the stress response and reactive oxygen species (ROS) production, while esmd1 is a suppressor of a pectin deficiency induced loss of adhesion.
34015001	8	81	theme	ROS	1594:1596	arg1	induction					1581:1589	the induction	1577:1589	the induction of ROS but not the transcriptional response induced by the elicitors Flagellin, Chitin and oligogalacturonides (OGs)	1577:1706	The WAK locus deletion does however affect the induction of ROS but not the transcriptional response induced by the elicitors Flagellin, Chitin and oligogalacturonides (OGs).
34015001	1	82	theme	cell	230:233	arg1	wall					235:238	the plant cell wall	220:238	the plant cell wall	220:238	Angiosperm cell adhesion is dependent on interactions between pectin polysaccharides which make up a significant portion of the plant cell wall.
34015001	7	83	theme	required	1430:1437	arg1	component					1439:1447	a required component	1428:1447	a required component of the potential ESMD1 signaling mechanism involved in the control of cell adhesion	1428:1531	Moreover, a WAK locus deletion allele has no effect on the ability of esmd1 to suppress an adhesion deficiency, indicating WAKs and their modification are not a required component of the potential ESMD1 signaling mechanism involved in the control of cell adhesion.
34015001	7	83	theme	required	1430:1437	arg1	WAKs					1392:1395	WAKs	1392:1395	WAKs	1392:1395	Moreover, a WAK locus deletion allele has no effect on the ability of esmd1 to suppress an adhesion deficiency, indicating WAKs and their modification are not a required component of the potential ESMD1 signaling mechanism involved in the control of cell adhesion.
34015001	7	83	theme	required	1430:1437	arg1	modification					1407:1418	their modification	1401:1418	their modification	1401:1418	Moreover, a WAK locus deletion allele has no effect on the ability of esmd1 to suppress an adhesion deficiency, indicating WAKs and their modification are not a required component of the potential ESMD1 signaling mechanism involved in the control of cell adhesion.
34015001	6	84	theme	Additional	1065:1074	arg1	analysis					1076:1083	Additional analysis	1065:1083	Additional analysis	1065:1083	Additional analysis suggests that mutations of the potential WAK EGF O-fucosylation site also abate the WAK2cTAP phenotype, yet only evidence for an N-linked but not O-linked sugar addition can be found.
34015001	8	85	theme	locus	1542:1546	arg1	deletion					1548:1555	The WAK locus deletion	1534:1555	The WAK locus deletion	1534:1555	The WAK locus deletion does however affect the induction of ROS but not the transcriptional response induced by the elicitors Flagellin, Chitin and oligogalacturonides (OGs).
33420994	5	0	theme	cytokines	808:816	arg1	concentrations					777:790	the concentrations	773:790	the concentrations of 23 different cytokines, chemokines, and growth factors in up to 76 samples	773:868	Final assay readout provides the concentrations of 23 different cytokines, chemokines, and growth factors in up to 76 samples.
33420994	1	1	theme	levels	154:159	arg1	analysis					127:134	The comprehensive analysis	109:134	The comprehensive analysis of serum cytokine levels	109:159	The comprehensive analysis of serum cytokine levels can be challenging due to low sample volumes and time consuming when using single-target methods like enzyme-linked immunosorbent assay (ELISA).
33420994	1	2	theme	single-target	236:248	arg1	methods					250:256	single-target methods	236:256	single-target methods	236:256	The comprehensive analysis of serum cytokine levels can be challenging due to low sample volumes and time consuming when using single-target methods like enzyme-linked immunosorbent assay (ELISA).
33420994	0	3	theme	Chemokines	77:86	arg1	Detection					53:61	the Detection	49:61	the Detection of Cytokines, Chemokines, and Growth Factors	49:106	Multiplex Fluorescent Bead-Based Immunoassay for the Detection of Cytokines, Chemokines, and Growth Factors.
33420994	4	4	theme	sandwich-like	717:729	arg1	composition					731:741	a sandwich-like composition	715:741	a sandwich-like composition	715:741	This assay is based on antibody-coupled fluorescent magnetic beads combined with biotinylated secondary detection antibody followed by fluorescent-tagged streptavidin in a sandwich-like composition.
33420994	0	5	theme	Growth	93:98	arg1	Factors					100:106	Growth Factors	93:106	Growth Factors	93:106	Multiplex Fluorescent Bead-Based Immunoassay for the Detection of Cytokines, Chemokines, and Growth Factors.
33420994	1	6	link	enzyme-linked	263:275	arg1	ELISA					298:302	ELISA	298:302	ELISA	298:302	The comprehensive analysis of serum cytokine levels can be challenging due to low sample volumes and time consuming when using single-target methods like enzyme-linked immunosorbent assay (ELISA).
33420994	1	6	link	enzyme-linked	263:275	arg1	assay					291:295	enzyme-linked immunosorbent assay	263:295	enzyme-linked immunosorbent assay (ELISA)	263:303	The comprehensive analysis of serum cytokine levels can be challenging due to low sample volumes and time consuming when using single-target methods like enzyme-linked immunosorbent assay (ELISA).
33420994	3	7	theme	96-well	529:535	arg1	format					537:542	a 96-well format	527:542	a 96-well format	527:542	Here we describe the use of a multiplex cytokine, chemokine, and growth factor assay for mouse cytokines in a 96-well format.
33420994	4	8	theme	fluorescent	585:595	arg1	beads					606:610	antibody-coupled fluorescent magnetic beads	568:610	antibody-coupled fluorescent magnetic beads combined with biotinylated secondary detection antibody followed by fluorescent-tagged streptavidin in a sandwich-like composition	568:741	This assay is based on antibody-coupled fluorescent magnetic beads combined with biotinylated secondary detection antibody followed by fluorescent-tagged streptavidin in a sandwich-like composition.
33420994	4	9	theme	biotinylated	626:637	arg1	antibody					659:666	biotinylated secondary detection antibody	626:666	biotinylated secondary detection antibody followed by fluorescent-tagged streptavidin in a sandwich-like composition	626:741	This assay is based on antibody-coupled fluorescent magnetic beads combined with biotinylated secondary detection antibody followed by fluorescent-tagged streptavidin in a sandwich-like composition.
33420994	3	10	theme	assay	498:502	arg1	use					440:442	the use	436:442	the use of a multiplex cytokine, chemokine, and growth factor assay for mouse cytokines	436:522	Here we describe the use of a multiplex cytokine, chemokine, and growth factor assay for mouse cytokines in a 96-well format.
33420994	1	11	theme	enzyme-linked	263:275	arg1	ELISA					298:302	ELISA	298:302	ELISA	298:302	The comprehensive analysis of serum cytokine levels can be challenging due to low sample volumes and time consuming when using single-target methods like enzyme-linked immunosorbent assay (ELISA).
33420994	1	11	theme	enzyme-linked	263:275	arg1	assay					291:295	enzyme-linked immunosorbent assay	263:295	enzyme-linked immunosorbent assay (ELISA)	263:303	The comprehensive analysis of serum cytokine levels can be challenging due to low sample volumes and time consuming when using single-target methods like enzyme-linked immunosorbent assay (ELISA).
33420994	3	12	theme	growth	484:489	arg1	assay					498:502	a multiplex cytokine, chemokine, and growth factor assay	447:502	a multiplex cytokine, chemokine, and growth factor assay	447:502	Here we describe the use of a multiplex cytokine, chemokine, and growth factor assay for mouse cytokines in a 96-well format.
33420994	3	13	theme	chemokine	469:477	arg1	assay					498:502	a multiplex cytokine, chemokine, and growth factor assay	447:502	a multiplex cytokine, chemokine, and growth factor assay	447:502	Here we describe the use of a multiplex cytokine, chemokine, and growth factor assay for mouse cytokines in a 96-well format.
33420994	0	14	theme	Factors	100:106	arg1	Detection					53:61	the Detection	49:61	the Detection of Cytokines, Chemokines, and Growth Factors	49:106	Multiplex Fluorescent Bead-Based Immunoassay for the Detection of Cytokines, Chemokines, and Growth Factors.
33420994	5	15	theme	factors	842:848	arg1	concentrations					777:790	the concentrations	773:790	the concentrations of 23 different cytokines, chemokines, and growth factors in up to 76 samples	773:868	Final assay readout provides the concentrations of 23 different cytokines, chemokines, and growth factors in up to 76 samples.
33420994	2	16	theme	sample	403:408	arg1	volumes					410:416	minimal sample volumes	395:416	minimal sample volumes	395:416	Bead-based detection systems allow the simultaneous detection of multiple analytes using minimal sample volumes.
33420994	0	17	theme	Fluorescent	10:20	arg1	Immunoassay					33:43	Multiplex Fluorescent Bead-Based Immunoassay	0:43	Multiplex Fluorescent Bead-Based Immunoassay for the Detection of Cytokines, Chemokines, and Growth Factors.	0:107	Multiplex Fluorescent Bead-Based Immunoassay for the Detection of Cytokines, Chemokines, and Growth Factors.
33420994	2	18	theme	detection	317:325	arg1	systems					327:333	Bead-based detection systems	306:333	Bead-based detection systems	306:333	Bead-based detection systems allow the simultaneous detection of multiple analytes using minimal sample volumes.
33420994	3	19	theme	mouse	508:512	arg1	cytokines					514:522	mouse cytokines	508:522	mouse cytokines	508:522	Here we describe the use of a multiplex cytokine, chemokine, and growth factor assay for mouse cytokines in a 96-well format.
33420994	2	20	theme	minimal	395:401	arg1	volumes					410:416	minimal sample volumes	395:416	minimal sample volumes	395:416	Bead-based detection systems allow the simultaneous detection of multiple analytes using minimal sample volumes.
33420994	0	21	theme	Multiplex	0:8	arg1	Immunoassay					33:43	Multiplex Fluorescent Bead-Based Immunoassay	0:43	Multiplex Fluorescent Bead-Based Immunoassay for the Detection of Cytokines, Chemokines, and Growth Factors.	0:107	Multiplex Fluorescent Bead-Based Immunoassay for the Detection of Cytokines, Chemokines, and Growth Factors.
33420994	1	22	theme	immunosorbent	277:289	arg1	ELISA					298:302	ELISA	298:302	ELISA	298:302	The comprehensive analysis of serum cytokine levels can be challenging due to low sample volumes and time consuming when using single-target methods like enzyme-linked immunosorbent assay (ELISA).
33420994	1	22	theme	immunosorbent	277:289	arg1	assay					291:295	enzyme-linked immunosorbent assay	263:295	enzyme-linked immunosorbent assay (ELISA)	263:303	The comprehensive analysis of serum cytokine levels can be challenging due to low sample volumes and time consuming when using single-target methods like enzyme-linked immunosorbent assay (ELISA).
33420994	2	23	theme	Bead-based	306:315	arg1	systems					327:333	Bead-based detection systems	306:333	Bead-based detection systems	306:333	Bead-based detection systems allow the simultaneous detection of multiple analytes using minimal sample volumes.
33420994	4	24	theme	fluorescent-tagged	680:697	arg1	streptavidin					699:710	fluorescent-tagged streptavidin	680:710	fluorescent-tagged streptavidin	680:710	This assay is based on antibody-coupled fluorescent magnetic beads combined with biotinylated secondary detection antibody followed by fluorescent-tagged streptavidin in a sandwich-like composition.
33420994	1	25	theme	low	187:189	arg1	volumes					198:204	low sample volumes	187:204	low sample volumes	187:204	The comprehensive analysis of serum cytokine levels can be challenging due to low sample volumes and time consuming when using single-target methods like enzyme-linked immunosorbent assay (ELISA).
33420994	0	26	theme	Bead-Based	22:31	arg1	Immunoassay					33:43	Multiplex Fluorescent Bead-Based Immunoassay	0:43	Multiplex Fluorescent Bead-Based Immunoassay for the Detection of Cytokines, Chemokines, and Growth Factors.	0:107	Multiplex Fluorescent Bead-Based Immunoassay for the Detection of Cytokines, Chemokines, and Growth Factors.
33420994	5	27	theme	Final	744:748	arg1	readout					756:762	Final assay readout	744:762	Final assay readout	744:762	Final assay readout provides the concentrations of 23 different cytokines, chemokines, and growth factors in up to 76 samples.
33420994	5	28	from	concentrations	777:790	arg1	samples					862:868	up to 76 samples	853:868	up to 76 samples	853:868	Final assay readout provides the concentrations of 23 different cytokines, chemokines, and growth factors in up to 76 samples.
33420994	1	29	theme	sample	191:196	arg1	volumes					198:204	low sample volumes	187:204	low sample volumes	187:204	The comprehensive analysis of serum cytokine levels can be challenging due to low sample volumes and time consuming when using single-target methods like enzyme-linked immunosorbent assay (ELISA).
33420994	5	30	theme	chemokines	819:828	arg1	concentrations					777:790	the concentrations	773:790	the concentrations of 23 different cytokines, chemokines, and growth factors in up to 76 samples	773:868	Final assay readout provides the concentrations of 23 different cytokines, chemokines, and growth factors in up to 76 samples.
33420994	3	31	theme	factor	491:496	arg1	assay					498:502	a multiplex cytokine, chemokine, and growth factor assay	447:502	a multiplex cytokine, chemokine, and growth factor assay	447:502	Here we describe the use of a multiplex cytokine, chemokine, and growth factor assay for mouse cytokines in a 96-well format.
33420994	5	32	theme	assay	750:754	arg1	readout					756:762	Final assay readout	744:762	Final assay readout	744:762	Final assay readout provides the concentrations of 23 different cytokines, chemokines, and growth factors in up to 76 samples.
33420994	3	33	theme	multiplex	449:457	arg1	cytokine					459:466	multiplex cytokine	449:466	multiplex cytokine	449:466	Here we describe the use of a multiplex cytokine, chemokine, and growth factor assay for mouse cytokines in a 96-well format.
33420994	3	34	theme	cytokine	459:466	arg1	assay					498:502	a multiplex cytokine, chemokine, and growth factor assay	447:502	a multiplex cytokine, chemokine, and growth factor assay	447:502	Here we describe the use of a multiplex cytokine, chemokine, and growth factor assay for mouse cytokines in a 96-well format.
33420994	5	35	theme	different	798:806	arg1	cytokines					808:816	23 different cytokines	795:816	23 different cytokines	795:816	Final assay readout provides the concentrations of 23 different cytokines, chemokines, and growth factors in up to 76 samples.
33420994	2	36	theme	simultaneous	345:356	arg1	detection					358:366	the simultaneous detection	341:366	the simultaneous detection of multiple analytes using minimal sample volumes	341:416	Bead-based detection systems allow the simultaneous detection of multiple analytes using minimal sample volumes.
33420994	5	37	theme	growth	835:840	arg1	factors					842:848	growth factors	835:848	growth factors	835:848	Final assay readout provides the concentrations of 23 different cytokines, chemokines, and growth factors in up to 76 samples.
33420994	1	38	theme	comprehensive	113:125	arg1	analysis					127:134	The comprehensive analysis	109:134	The comprehensive analysis of serum cytokine levels	109:159	The comprehensive analysis of serum cytokine levels can be challenging due to low sample volumes and time consuming when using single-target methods like enzyme-linked immunosorbent assay (ELISA).
33420994	1	39	theme	serum	139:143	arg1	levels					154:159	serum cytokine levels	139:159	serum cytokine levels	139:159	The comprehensive analysis of serum cytokine levels can be challenging due to low sample volumes and time consuming when using single-target methods like enzyme-linked immunosorbent assay (ELISA).
33420994	1	40	theme	cytokine	145:152	arg1	levels					154:159	serum cytokine levels	139:159	serum cytokine levels	139:159	The comprehensive analysis of serum cytokine levels can be challenging due to low sample volumes and time consuming when using single-target methods like enzyme-linked immunosorbent assay (ELISA).
33420994	4	41	theme	magnetic	597:604	arg1	beads					606:610	antibody-coupled fluorescent magnetic beads	568:610	antibody-coupled fluorescent magnetic beads combined with biotinylated secondary detection antibody followed by fluorescent-tagged streptavidin in a sandwich-like composition	568:741	This assay is based on antibody-coupled fluorescent magnetic beads combined with biotinylated secondary detection antibody followed by fluorescent-tagged streptavidin in a sandwich-like composition.
33420994	4	42	theme	detection	649:657	arg1	antibody					659:666	biotinylated secondary detection antibody	626:666	biotinylated secondary detection antibody followed by fluorescent-tagged streptavidin in a sandwich-like composition	626:741	This assay is based on antibody-coupled fluorescent magnetic beads combined with biotinylated secondary detection antibody followed by fluorescent-tagged streptavidin in a sandwich-like composition.
33420994	5	43	dep	76	859:860	arg1	to					856:857	to	856:857	to	856:857	Final assay readout provides the concentrations of 23 different cytokines, chemokines, and growth factors in up to 76 samples.
33420994	4	44	theme	secondary	639:647	arg1	antibody					659:666	biotinylated secondary detection antibody	626:666	biotinylated secondary detection antibody followed by fluorescent-tagged streptavidin in a sandwich-like composition	626:741	This assay is based on antibody-coupled fluorescent magnetic beads combined with biotinylated secondary detection antibody followed by fluorescent-tagged streptavidin in a sandwich-like composition.
33420994	2	45	theme	analytes	380:387	arg1	detection					358:366	the simultaneous detection	341:366	the simultaneous detection of multiple analytes using minimal sample volumes	341:416	Bead-based detection systems allow the simultaneous detection of multiple analytes using minimal sample volumes.
33420994	4	46	theme	antibody-coupled	568:583	arg1	beads					606:610	antibody-coupled fluorescent magnetic beads	568:610	antibody-coupled fluorescent magnetic beads combined with biotinylated secondary detection antibody followed by fluorescent-tagged streptavidin in a sandwich-like composition	568:741	This assay is based on antibody-coupled fluorescent magnetic beads combined with biotinylated secondary detection antibody followed by fluorescent-tagged streptavidin in a sandwich-like composition.
33420994	0	47	theme	Cytokines	66:74	arg1	Detection					53:61	the Detection	49:61	the Detection of Cytokines, Chemokines, and Growth Factors	49:106	Multiplex Fluorescent Bead-Based Immunoassay for the Detection of Cytokines, Chemokines, and Growth Factors.
33420994	2	48	theme	multiple	371:378	arg1	analytes					380:387	multiple analytes	371:387	multiple analytes using minimal sample volumes	371:416	Bead-based detection systems allow the simultaneous detection of multiple analytes using minimal sample volumes.
33400640	11	0	theme	strain	1546:1551	arg1	CC-CFT486T					1553:1562	strain CC-CFT486T	1546:1562	strain CC-CFT486T	1546:1562	Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence and ANI analyses, strain CC-CFT486T is proposed to represent a novel Aeromicrobium species, for which the name Aeromicrobium terrae sp.
33400640	10	1	theme	quinone	1361:1367	arg1	menaquinone					1373:1383	the predominant quinone was menaquinone 9	1345:1385	the predominant quinone was menaquinone 9 (MK-9)	1345:1392	The DNA G+C content was 70.6 mol% and the predominant quinone was menaquinone 9 (MK-9).
33400640	10	1	theme	quinone	1361:1367	arg1	MK-9					1388:1391	MK-9	1388:1391	MK-9	1388:1391	The DNA G+C content was 70.6 mol% and the predominant quinone was menaquinone 9 (MK-9).
33400640	11	2	theme	gene	1514:1517	arg1	sequence					1519:1526	comparative 16S rRNA gene sequence	1493:1526	comparative 16S rRNA gene sequence	1493:1526	Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence and ANI analyses, strain CC-CFT486T is proposed to represent a novel Aeromicrobium species, for which the name Aeromicrobium terrae sp.
33400640	11	3	theme	distinct	1408:1415	arg1	traits					1461:1466	its distinct phylogenetic, phenotypic and chemotaxonomic traits	1404:1466	its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence and ANI analyses	1404:1543	Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence and ANI analyses, strain CC-CFT486T is proposed to represent a novel Aeromicrobium species, for which the name Aeromicrobium terrae sp.
33400640	3	4	theme	polar	291:295	arg1	flagella					297:304	polar flagella	291:304	polar flagella	291:304	Cells of strain CC-CFT486T were short rods, motile with polar flagella, catalase-positive and oxidase-positive.
33400640	11	5	theme	ANI	1532:1534	arg1	analyses					1536:1543	ANI analyses	1532:1543	ANI analyses	1532:1543	Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence and ANI analyses, strain CC-CFT486T is proposed to represent a novel Aeromicrobium species, for which the name Aeromicrobium terrae sp.
33400640	6	6	theme	identity	762:769	arg1	values					777:782	Average nucleotide identity (ANI) values	743:782	Average nucleotide identity (ANI) values	743:782	Average nucleotide identity (ANI) values were 70.6-77.8 % (n=11) compared within the type strains of the genus Aeromicrobium.
33400640	5	7	theme	%	562:562	arg1	identity					573:580	97.0 % sequence identity	557:580	97.0 % sequence identity	557:580	Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-CFT486T associated with Aeromicrobium panacisoli (97.0 % sequence identity), Aeromicrobium lacus (97.0 %), Aeromicrobium erythreum (96.8 %) and Aeromicrobium alkaliterrae (96.8 %), and lower sequence similarity values to other species.
33400640	5	7	theme	%	562:562	arg1	panacisoli					545:554	Aeromicrobium panacisoli	531:554	Aeromicrobium panacisoli (97.0 % sequence identity)	531:581	Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-CFT486T associated with Aeromicrobium panacisoli (97.0 % sequence identity), Aeromicrobium lacus (97.0 %), Aeromicrobium erythreum (96.8 %) and Aeromicrobium alkaliterrae (96.8 %), and lower sequence similarity values to other species.
33400640	9	8	contain	contained	1225:1233	arg2	acid					1253:1256	ll-diaminopimelic acid	1235:1256	ll-diaminopimelic acid (ll-DAP)	1235:1265	The cell wall peptidoglycan of strains CC-CFT486T contained ll-diaminopimelic acid (ll-DAP) and the major polyamine was spermidine.
33400640	9	8	contain	contained	1225:1233	arg2	spermidine					1295:1304	the major polyamine was spermidine	1271:1304	the major polyamine was spermidine	1271:1304	The cell wall peptidoglycan of strains CC-CFT486T contained ll-diaminopimelic acid (ll-DAP) and the major polyamine was spermidine.
33400640	9	8	contain	contained	1225:1233	arg1	peptidoglycan					1189:1201	The cell wall peptidoglycan	1175:1201	The cell wall peptidoglycan of strains	1175:1212	The cell wall peptidoglycan of strains CC-CFT486T contained ll-diaminopimelic acid (ll-DAP) and the major polyamine was spermidine.
33400640	9	8	contain	contained	1225:1233	arg2	ll-DAP					1259:1264	ll-DAP	1259:1264	ll-DAP	1259:1264	The cell wall peptidoglycan of strains CC-CFT486T contained ll-diaminopimelic acid (ll-DAP) and the major polyamine was spermidine.
33400640	10	9	theme	predominant	1349:1359	arg1	menaquinone					1373:1383	the predominant quinone was menaquinone 9	1345:1385	the predominant quinone was menaquinone 9 (MK-9)	1345:1392	The DNA G+C content was 70.6 mol% and the predominant quinone was menaquinone 9 (MK-9).
33400640	10	9	theme	predominant	1349:1359	arg1	MK-9					1388:1391	MK-9	1388:1391	MK-9	1388:1391	The DNA G+C content was 70.6 mol% and the predominant quinone was menaquinone 9 (MK-9).
33400640	3	10	theme	oxidase-positive	329:344	arg1	rods					273:276	short rods	267:276	short rods	267:276	Cells of strain CC-CFT486T were short rods, motile with polar flagella, catalase-positive and oxidase-positive.
33400640	9	11	theme	strains	1206:1212	arg1	peptidoglycan					1189:1201	The cell wall peptidoglycan	1175:1201	The cell wall peptidoglycan of strains	1175:1212	The cell wall peptidoglycan of strains CC-CFT486T contained ll-diaminopimelic acid (ll-DAP) and the major polyamine was spermidine.
33400640	7	12	theme	predominant	950:960	arg1	acids					968:972	the predominant fatty acids	946:972	the predominant fatty acids	946:972	Strain CC-CFT486T contained C16 : 0, C17 : 0, C17 : 1 ω8c and C18 : 1 ω9c as the predominant fatty acids.
33400640	7	12	theme	predominant	950:960	arg1	C17 					915:918	C17 	915:918	C17 	915:918	Strain CC-CFT486T contained C16 : 0, C17 : 0, C17 : 1 ω8c and C18 : 1 ω9c as the predominant fatty acids.
33400640	7	12	theme	predominant	950:960	arg1	ω9c					939:941	ω9c	939:941	ω9c	939:941	Strain CC-CFT486T contained C16 : 0, C17 : 0, C17 : 1 ω8c and C18 : 1 ω9c as the predominant fatty acids.
33400640	7	12	theme	predominant	950:960	arg1	C17 					906:909	C17 	906:909	C17 	906:909	Strain CC-CFT486T contained C16 : 0, C17 : 0, C17 : 1 ω8c and C18 : 1 ω9c as the predominant fatty acids.
33400640	7	12	theme	predominant	950:960	arg1	ω8c					923:925	ω8c	923:925	ω8c	923:925	Strain CC-CFT486T contained C16 : 0, C17 : 0, C17 : 1 ω8c and C18 : 1 ω9c as the predominant fatty acids.
33400640	7	12	theme	predominant	950:960	arg1	C16 					897:900	C16 	897:900	C16 	897:900	Strain CC-CFT486T contained C16 : 0, C17 : 0, C17 : 1 ω8c and C18 : 1 ω9c as the predominant fatty acids.
33400640	5	13	theme	sequence	564:571	arg1	identity					573:580	97.0 % sequence identity	557:580	97.0 % sequence identity	557:580	Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-CFT486T associated with Aeromicrobium panacisoli (97.0 % sequence identity), Aeromicrobium lacus (97.0 %), Aeromicrobium erythreum (96.8 %) and Aeromicrobium alkaliterrae (96.8 %), and lower sequence similarity values to other species.
33400640	5	13	theme	sequence	564:571	arg1	panacisoli					545:554	Aeromicrobium panacisoli	531:554	Aeromicrobium panacisoli (97.0 % sequence identity)	531:581	Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-CFT486T associated with Aeromicrobium panacisoli (97.0 % sequence identity), Aeromicrobium lacus (97.0 %), Aeromicrobium erythreum (96.8 %) and Aeromicrobium alkaliterrae (96.8 %), and lower sequence similarity values to other species.
33400640	12	14	theme	strain	1675:1680	arg1	33499T					1709:1714	type strain CC-CFT486T=BCRC 81217T=JCM 33499T	1670:1714	type strain CC-CFT486T=BCRC 81217T=JCM 33499T	1670:1714	nov. (type strain CC-CFT486T=BCRC 81217T=JCM 33499T).
33400640	11	15	theme	novel	1591:1595	arg1	species					1611:1617	a novel Aeromicrobium species	1589:1617	a novel Aeromicrobium species	1589:1617	Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence and ANI analyses, strain CC-CFT486T is proposed to represent a novel Aeromicrobium species, for which the name Aeromicrobium terrae sp.
33400640	11	16	theme	sequence	1519:1526	arg1	results					1482:1488	results	1482:1488	results of comparative 16S rRNA gene sequence and ANI analyses	1482:1543	Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence and ANI analyses, strain CC-CFT486T is proposed to represent a novel Aeromicrobium species, for which the name Aeromicrobium terrae sp.
33400640	7	17	theme	fatty	962:966	arg1	acids					968:972	the predominant fatty acids	946:972	the predominant fatty acids	946:972	Strain CC-CFT486T contained C16 : 0, C17 : 0, C17 : 1 ω8c and C18 : 1 ω9c as the predominant fatty acids.
33400640	7	17	theme	fatty	962:966	arg1	C17 					915:918	C17 	915:918	C17 	915:918	Strain CC-CFT486T contained C16 : 0, C17 : 0, C17 : 1 ω8c and C18 : 1 ω9c as the predominant fatty acids.
33400640	7	17	theme	fatty	962:966	arg1	ω9c					939:941	ω9c	939:941	ω9c	939:941	Strain CC-CFT486T contained C16 : 0, C17 : 0, C17 : 1 ω8c and C18 : 1 ω9c as the predominant fatty acids.
33400640	7	17	theme	fatty	962:966	arg1	C17 					906:909	C17 	906:909	C17 	906:909	Strain CC-CFT486T contained C16 : 0, C17 : 0, C17 : 1 ω8c and C18 : 1 ω9c as the predominant fatty acids.
33400640	7	17	theme	fatty	962:966	arg1	ω8c					923:925	ω8c	923:925	ω8c	923:925	Strain CC-CFT486T contained C16 : 0, C17 : 0, C17 : 1 ω8c and C18 : 1 ω9c as the predominant fatty acids.
33400640	7	17	theme	fatty	962:966	arg1	C16 					897:900	C16 	897:900	C16 	897:900	Strain CC-CFT486T contained C16 : 0, C17 : 0, C17 : 1 ω8c and C18 : 1 ω9c as the predominant fatty acids.
33400640	11	18	theme	phylogenetic	1417:1428	arg1	traits					1461:1466	its distinct phylogenetic, phenotypic and chemotaxonomic traits	1404:1466	its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence and ANI analyses	1404:1543	Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence and ANI analyses, strain CC-CFT486T is proposed to represent a novel Aeromicrobium species, for which the name Aeromicrobium terrae sp.
33400640	1	19	theme	maize	47:51	arg1	field					53:57	a maize field	45:57	a maize field	45:57	nov., isolated from a maize field.
33400640	3	20	theme	catalase-positive	307:323	arg1	rods					273:276	short rods	267:276	short rods	267:276	Cells of strain CC-CFT486T were short rods, motile with polar flagella, catalase-positive and oxidase-positive.
33400640	12	21	dep	nov	1664:1666	arg1	33499T					1709:1714	type strain CC-CFT486T=BCRC 81217T=JCM 33499T	1670:1714	type strain CC-CFT486T=BCRC 81217T=JCM 33499T	1670:1714	nov. (type strain CC-CFT486T=BCRC 81217T=JCM 33499T).
33400640	8	22	theme	unknown	1152:1158	arg1	phospholipids					1160:1172	three unknown phospholipids	1146:1172	three unknown phospholipids	1146:1172	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, two unidentified aminophospholipids and three unknown phospholipids.
33400640	5	23	theme	similarity	707:716	arg1	values					718:723	lower sequence similarity values	692:723	lower sequence similarity values	692:723	Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-CFT486T associated with Aeromicrobium panacisoli (97.0 % sequence identity), Aeromicrobium lacus (97.0 %), Aeromicrobium erythreum (96.8 %) and Aeromicrobium alkaliterrae (96.8 %), and lower sequence similarity values to other species.
33400640	12	24	theme	81217T=JCM	1698:1707	arg1	33499T					1709:1714	type strain CC-CFT486T=BCRC 81217T=JCM 33499T	1670:1714	type strain CC-CFT486T=BCRC 81217T=JCM 33499T	1670:1714	nov. (type strain CC-CFT486T=BCRC 81217T=JCM 33499T).
33400640	2	25	theme	Gram-stain-positive	119:137	arg1	bacterium					139:147	a Gram-stain-positive bacterium	117:147	a Gram-stain-positive bacterium	117:147	A polyphasic taxonomic approach was used to characterize a Gram-stain-positive bacterium, designated strain CC-CFT486T, isolated from soil sampled in a maize field in Taiwan.
33400640	9	26	theme	cell	1179:1182	arg1	peptidoglycan					1189:1201	The cell wall peptidoglycan	1175:1201	The cell wall peptidoglycan of strains	1175:1212	The cell wall peptidoglycan of strains CC-CFT486T contained ll-diaminopimelic acid (ll-DAP) and the major polyamine was spermidine.
33400640	5	27	theme	Aeromicrobium	531:543	arg1	lacus					598:602	Aeromicrobium lacus	584:602	Aeromicrobium lacus (97.0 %)	584:611	Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-CFT486T associated with Aeromicrobium panacisoli (97.0 % sequence identity), Aeromicrobium lacus (97.0 %), Aeromicrobium erythreum (96.8 %) and Aeromicrobium alkaliterrae (96.8 %), and lower sequence similarity values to other species.
33400640	5	27	theme	Aeromicrobium	531:543	arg1	alkaliterrae					665:676	Aeromicrobium alkaliterrae	651:676	Aeromicrobium alkaliterrae (96.8 %)	651:685	Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-CFT486T associated with Aeromicrobium panacisoli (97.0 % sequence identity), Aeromicrobium lacus (97.0 %), Aeromicrobium erythreum (96.8 %) and Aeromicrobium alkaliterrae (96.8 %), and lower sequence similarity values to other species.
33400640	5	27	theme	Aeromicrobium	531:543	arg1	identity					573:580	97.0 % sequence identity	557:580	97.0 % sequence identity	557:580	Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-CFT486T associated with Aeromicrobium panacisoli (97.0 % sequence identity), Aeromicrobium lacus (97.0 %), Aeromicrobium erythreum (96.8 %) and Aeromicrobium alkaliterrae (96.8 %), and lower sequence similarity values to other species.
33400640	5	27	theme	Aeromicrobium	531:543	arg1	panacisoli					545:554	Aeromicrobium panacisoli	531:554	Aeromicrobium panacisoli (97.0 % sequence identity)	531:581	Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-CFT486T associated with Aeromicrobium panacisoli (97.0 % sequence identity), Aeromicrobium lacus (97.0 %), Aeromicrobium erythreum (96.8 %) and Aeromicrobium alkaliterrae (96.8 %), and lower sequence similarity values to other species.
33400640	5	27	theme	Aeromicrobium	531:543	arg1	erythreum					628:636	Aeromicrobium erythreum	614:636	Aeromicrobium erythreum (96.8 %)	614:645	Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-CFT486T associated with Aeromicrobium panacisoli (97.0 % sequence identity), Aeromicrobium lacus (97.0 %), Aeromicrobium erythreum (96.8 %) and Aeromicrobium alkaliterrae (96.8 %), and lower sequence similarity values to other species.
33400640	2	28	from	field	218:222	arg1	Taiwan					227:232	Taiwan	227:232	Taiwan	227:232	A polyphasic taxonomic approach was used to characterize a Gram-stain-positive bacterium, designated strain CC-CFT486T, isolated from soil sampled in a maize field in Taiwan.
33400640	9	29	theme	polyamine	1281:1289	arg1	spermidine					1295:1304	the major polyamine was spermidine	1271:1304	the major polyamine was spermidine	1271:1304	The cell wall peptidoglycan of strains CC-CFT486T contained ll-diaminopimelic acid (ll-DAP) and the major polyamine was spermidine.
33400640	0	30	theme	terrae	14:19	arg1	sp					21:22	Aeromicrobium terrae sp	0:22	Aeromicrobium terrae sp.	0:23	Aeromicrobium terrae sp.
33400640	5	31	theme	lower	692:696	arg1	values					718:723	lower sequence similarity values	692:723	lower sequence similarity values	692:723	Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-CFT486T associated with Aeromicrobium panacisoli (97.0 % sequence identity), Aeromicrobium lacus (97.0 %), Aeromicrobium erythreum (96.8 %) and Aeromicrobium alkaliterrae (96.8 %), and lower sequence similarity values to other species.
33400640	10	32	theme	was	1369:1371	arg1	menaquinone					1373:1383	the predominant quinone was menaquinone 9	1345:1385	the predominant quinone was menaquinone 9 (MK-9)	1345:1392	The DNA G+C content was 70.6 mol% and the predominant quinone was menaquinone 9 (MK-9).
33400640	10	32	theme	was	1369:1371	arg1	MK-9					1388:1391	MK-9	1388:1391	MK-9	1388:1391	The DNA G+C content was 70.6 mol% and the predominant quinone was menaquinone 9 (MK-9).
33400640	4	33	theme	Optimal	347:353	arg1	growth					355:360	Optimal growth	347:360	Optimal growth	347:360	Optimal growth occurred at 30 °С, pH 8 and 1 % NaCl.
33400640	5	34	theme	distinct	457:464	arg1	position					476:483	a distinct taxonomic position	455:483	a distinct taxonomic position attained by strain CC-CFT486T associated with Aeromicrobium panacisoli (97.0 % sequence identity), Aeromicrobium lacus (97.0 %), Aeromicrobium erythreum (96.8 %) and Aeromicrobium alkaliterrae (96.8 %), and lower sequence similarity values to other species	455:740	Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-CFT486T associated with Aeromicrobium panacisoli (97.0 % sequence identity), Aeromicrobium lacus (97.0 %), Aeromicrobium erythreum (96.8 %) and Aeromicrobium alkaliterrae (96.8 %), and lower sequence similarity values to other species.
33400640	2	35	attach	isolated	180:187	arg2	bacterium					139:147	a Gram-stain-positive bacterium	117:147	a Gram-stain-positive bacterium	117:147	A polyphasic taxonomic approach was used to characterize a Gram-stain-positive bacterium, designated strain CC-CFT486T, isolated from soil sampled in a maize field in Taiwan.
33400640	2	35	attach	isolated	180:187	arg1	soil					194:197	soil	194:197	soil sampled in a maize field in Taiwan	194:232	A polyphasic taxonomic approach was used to characterize a Gram-stain-positive bacterium, designated strain CC-CFT486T, isolated from soil sampled in a maize field in Taiwan.
33400640	0	36	theme	Aeromicrobium	0:12	arg1	sp					21:22	Aeromicrobium terrae sp	0:22	Aeromicrobium terrae sp.	0:23	Aeromicrobium terrae sp.
33400640	11	37	theme	phenotypic	1431:1440	arg1	traits					1461:1466	its distinct phylogenetic, phenotypic and chemotaxonomic traits	1404:1466	its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence and ANI analyses	1404:1543	Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence and ANI analyses, strain CC-CFT486T is proposed to represent a novel Aeromicrobium species, for which the name Aeromicrobium terrae sp.
33400640	6	38	theme	nucleotide	751:760	arg1	ANI					772:774	ANI	772:774	ANI	772:774	Average nucleotide identity (ANI) values were 70.6-77.8 % (n=11) compared within the type strains of the genus Aeromicrobium.
33400640	6	38	theme	nucleotide	751:760	arg1	identity					762:769	Average nucleotide identity	743:769	Average nucleotide identity (ANI) values	743:782	Average nucleotide identity (ANI) values were 70.6-77.8 % (n=11) compared within the type strains of the genus Aeromicrobium.
33400640	11	39	theme	chemotaxonomic	1446:1459	arg1	traits					1461:1466	its distinct phylogenetic, phenotypic and chemotaxonomic traits	1404:1466	its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence and ANI analyses	1404:1543	Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence and ANI analyses, strain CC-CFT486T is proposed to represent a novel Aeromicrobium species, for which the name Aeromicrobium terrae sp.
33400640	3	40	with	motile	279:284	arg1	flagella					297:304	polar flagella	291:304	polar flagella	291:304	Cells of strain CC-CFT486T were short rods, motile with polar flagella, catalase-positive and oxidase-positive.
33400640	3	41	theme	CC-CFT486T	251:260	arg1	Cells					235:239	Cells	235:239	Cells of strain CC-CFT486T	235:260	Cells of strain CC-CFT486T were short rods, motile with polar flagella, catalase-positive and oxidase-positive.
33400640	10	42	theme	G+C	1315:1317	arg1	content					1319:1325	The DNA G+C content	1307:1325	The DNA G+C content	1307:1325	The DNA G+C content was 70.6 mol% and the predominant quinone was menaquinone 9 (MK-9).
33400640	10	42	theme	G+C	1315:1317	arg1	%					1339:1339	70.6 mol%	1331:1339	70.6 mol%	1331:1339	The DNA G+C content was 70.6 mol% and the predominant quinone was menaquinone 9 (MK-9).
33400640	6	43	theme	Average	743:749	arg1	ANI					772:774	ANI	772:774	ANI	772:774	Average nucleotide identity (ANI) values were 70.6-77.8 % (n=11) compared within the type strains of the genus Aeromicrobium.
33400640	6	43	theme	Average	743:749	arg1	identity					762:769	Average nucleotide identity	743:769	Average nucleotide identity (ANI) values	743:782	Average nucleotide identity (ANI) values were 70.6-77.8 % (n=11) compared within the type strains of the genus Aeromicrobium.
33400640	10	44	theme	DNA	1311:1313	arg1	content					1319:1325	The DNA G+C content	1307:1325	The DNA G+C content	1307:1325	The DNA G+C content was 70.6 mol% and the predominant quinone was menaquinone 9 (MK-9).
33400640	10	44	theme	DNA	1311:1313	arg1	%					1339:1339	70.6 mol%	1331:1339	70.6 mol%	1331:1339	The DNA G+C content was 70.6 mol% and the predominant quinone was menaquinone 9 (MK-9).
33400640	5	45	theme	16S	431:433	arg1	genes					440:444	16S rRNA genes	431:444	16S rRNA genes	431:444	Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-CFT486T associated with Aeromicrobium panacisoli (97.0 % sequence identity), Aeromicrobium lacus (97.0 %), Aeromicrobium erythreum (96.8 %) and Aeromicrobium alkaliterrae (96.8 %), and lower sequence similarity values to other species.
33400640	9	46	theme	wall	1184:1187	arg1	peptidoglycan					1189:1201	The cell wall peptidoglycan	1175:1201	The cell wall peptidoglycan of strains	1175:1212	The cell wall peptidoglycan of strains CC-CFT486T contained ll-diaminopimelic acid (ll-DAP) and the major polyamine was spermidine.
33400640	5	47	theme	sequence	698:705	arg1	values					718:723	lower sequence similarity values	692:723	lower sequence similarity values	692:723	Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-CFT486T associated with Aeromicrobium panacisoli (97.0 % sequence identity), Aeromicrobium lacus (97.0 %), Aeromicrobium erythreum (96.8 %) and Aeromicrobium alkaliterrae (96.8 %), and lower sequence similarity values to other species.
33400640	6	48	theme	Aeromicrobium	854:866	arg1	strains					833:839	the type strains	824:839	the type strains of the genus Aeromicrobium	824:866	Average nucleotide identity (ANI) values were 70.6-77.8 % (n=11) compared within the type strains of the genus Aeromicrobium.
33400640	11	49	theme	Aeromicrobium	1597:1609	arg1	species					1611:1617	a novel Aeromicrobium species	1589:1617	a novel Aeromicrobium species	1589:1617	Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence and ANI analyses, strain CC-CFT486T is proposed to represent a novel Aeromicrobium species, for which the name Aeromicrobium terrae sp.
33400640	5	50	theme	rRNA	435:438	arg1	genes					440:444	16S rRNA genes	431:444	16S rRNA genes	431:444	Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-CFT486T associated with Aeromicrobium panacisoli (97.0 % sequence identity), Aeromicrobium lacus (97.0 %), Aeromicrobium erythreum (96.8 %) and Aeromicrobium alkaliterrae (96.8 %), and lower sequence similarity values to other species.
33400640	2	51	used	used	96:99	arg2	approach					83:90	A polyphasic taxonomic approach	60:90	A polyphasic taxonomic approach	60:90	A polyphasic taxonomic approach was used to characterize a Gram-stain-positive bacterium, designated strain CC-CFT486T, isolated from soil sampled in a maize field in Taiwan.
33400640	6	52	theme	genus	848:852	arg1	Aeromicrobium					854:866	the genus Aeromicrobium	844:866	the genus Aeromicrobium	844:866	Average nucleotide identity (ANI) values were 70.6-77.8 % (n=11) compared within the type strains of the genus Aeromicrobium.
33400640	3	53	theme	strain	244:249	arg1	CC-CFT486T					251:260	strain CC-CFT486T	244:260	strain CC-CFT486T	244:260	Cells of strain CC-CFT486T were short rods, motile with polar flagella, catalase-positive and oxidase-positive.
33400640	2	54	theme	strain	161:166	arg1	CC-CFT486T					168:177	strain CC-CFT486T	161:177	strain CC-CFT486T	161:177	A polyphasic taxonomic approach was used to characterize a Gram-stain-positive bacterium, designated strain CC-CFT486T, isolated from soil sampled in a maize field in Taiwan.
33400640	2	55	theme	maize	212:216	arg1	field					218:222	a maize field	210:222	a maize field in Taiwan	210:232	A polyphasic taxonomic approach was used to characterize a Gram-stain-positive bacterium, designated strain CC-CFT486T, isolated from soil sampled in a maize field in Taiwan.
33400640	5	56	theme	strain	497:502	arg1	CC-CFT486T					504:513	strain CC-CFT486T	497:513	strain CC-CFT486T associated with Aeromicrobium panacisoli (97.0 % sequence identity), Aeromicrobium lacus (97.0 %), Aeromicrobium erythreum (96.8 %) and Aeromicrobium alkaliterrae (96.8 %), and lower sequence similarity values to other species	497:740	Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-CFT486T associated with Aeromicrobium panacisoli (97.0 % sequence identity), Aeromicrobium lacus (97.0 %), Aeromicrobium erythreum (96.8 %) and Aeromicrobium alkaliterrae (96.8 %), and lower sequence similarity values to other species.
33400640	11	57	theme	16S	1505:1507	arg1	rRNA					1509:1512	comparative 16S rRNA	1493:1512	comparative 16S rRNA gene sequence	1493:1526	Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence and ANI analyses, strain CC-CFT486T is proposed to represent a novel Aeromicrobium species, for which the name Aeromicrobium terrae sp.
33400640	8	58	theme	unidentified	1110:1121	arg1	aminophospholipids					1123:1140	two unidentified aminophospholipids	1106:1140	two unidentified aminophospholipids	1106:1140	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, two unidentified aminophospholipids and three unknown phospholipids.
33400640	2	59	theme	taxonomic	73:81	arg1	approach					83:90	A polyphasic taxonomic approach	60:90	A polyphasic taxonomic approach	60:90	A polyphasic taxonomic approach was used to characterize a Gram-stain-positive bacterium, designated strain CC-CFT486T, isolated from soil sampled in a maize field in Taiwan.
33400640	9	60	theme	ll-diaminopimelic	1235:1251	arg1	ll-DAP					1259:1264	ll-DAP	1259:1264	ll-DAP	1259:1264	The cell wall peptidoglycan of strains CC-CFT486T contained ll-diaminopimelic acid (ll-DAP) and the major polyamine was spermidine.
33400640	9	60	theme	ll-diaminopimelic	1235:1251	arg1	acid					1253:1256	ll-diaminopimelic acid	1235:1256	ll-diaminopimelic acid (ll-DAP)	1235:1265	The cell wall peptidoglycan of strains CC-CFT486T contained ll-diaminopimelic acid (ll-DAP) and the major polyamine was spermidine.
33400640	11	61	theme	analyses	1536:1543	arg1	results					1482:1488	results	1482:1488	results of comparative 16S rRNA gene sequence and ANI analyses	1482:1543	Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence and ANI analyses, strain CC-CFT486T is proposed to represent a novel Aeromicrobium species, for which the name Aeromicrobium terrae sp.
33400640	2	62	theme	polyphasic	62:71	arg1	approach					83:90	A polyphasic taxonomic approach	60:90	A polyphasic taxonomic approach	60:90	A polyphasic taxonomic approach was used to characterize a Gram-stain-positive bacterium, designated strain CC-CFT486T, isolated from soil sampled in a maize field in Taiwan.
33400640	3	63	theme	motile	279:284	arg1	rods					273:276	short rods	267:276	short rods	267:276	Cells of strain CC-CFT486T were short rods, motile with polar flagella, catalase-positive and oxidase-positive.
33400640	8	64	theme	lipid	985:989	arg1	profile					991:997	The polar lipid profile	975:997	The polar lipid profile	975:997	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, two unidentified aminophospholipids and three unknown phospholipids.
33400640	9	65	theme	was	1291:1293	arg1	spermidine					1295:1304	the major polyamine was spermidine	1271:1304	the major polyamine was spermidine	1271:1304	The cell wall peptidoglycan of strains CC-CFT486T contained ll-diaminopimelic acid (ll-DAP) and the major polyamine was spermidine.
33400640	3	66	theme	short	267:271	arg1	rods					273:276	short rods	267:276	short rods	267:276	Cells of strain CC-CFT486T were short rods, motile with polar flagella, catalase-positive and oxidase-positive.
33400640	10	67	theme	70.6 mol	1331:1338	arg1	content					1319:1325	The DNA G+C content	1307:1325	The DNA G+C content	1307:1325	The DNA G+C content was 70.6 mol% and the predominant quinone was menaquinone 9 (MK-9).
33400640	10	67	theme	70.6 mol	1331:1338	arg1	%					1339:1339	70.6 mol%	1331:1339	70.6 mol%	1331:1339	The DNA G+C content was 70.6 mol% and the predominant quinone was menaquinone 9 (MK-9).
33400640	12	68	theme	CC-CFT486T=BCRC	1682:1696	arg1	33499T					1709:1714	type strain CC-CFT486T=BCRC 81217T=JCM 33499T	1670:1714	type strain CC-CFT486T=BCRC 81217T=JCM 33499T	1670:1714	nov. (type strain CC-CFT486T=BCRC 81217T=JCM 33499T).
33400640	11	69	theme	Aeromicrobium	1639:1651	arg1	sp					1660:1661	the name Aeromicrobium terrae sp	1630:1661	the name Aeromicrobium terrae sp	1630:1661	Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence and ANI analyses, strain CC-CFT486T is proposed to represent a novel Aeromicrobium species, for which the name Aeromicrobium terrae sp.
33400640	4	70	theme	%	392:392	arg1	 NaCl					393:397	1 % NaCl	390:397	1 % NaCl	390:397	Optimal growth occurred at 30 °С, pH 8 and 1 % NaCl.
33400640	11	71	theme	comparative	1493:1503	arg1	rRNA					1509:1512	comparative 16S rRNA	1493:1512	comparative 16S rRNA gene sequence	1493:1526	Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence and ANI analyses, strain CC-CFT486T is proposed to represent a novel Aeromicrobium species, for which the name Aeromicrobium terrae sp.
33400640	5	72	theme	taxonomic	466:474	arg1	position					476:483	a distinct taxonomic position	455:483	a distinct taxonomic position attained by strain CC-CFT486T associated with Aeromicrobium panacisoli (97.0 % sequence identity), Aeromicrobium lacus (97.0 %), Aeromicrobium erythreum (96.8 %) and Aeromicrobium alkaliterrae (96.8 %), and lower sequence similarity values to other species	455:740	Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-CFT486T associated with Aeromicrobium panacisoli (97.0 % sequence identity), Aeromicrobium lacus (97.0 %), Aeromicrobium erythreum (96.8 %) and Aeromicrobium alkaliterrae (96.8 %), and lower sequence similarity values to other species.
33400640	5	73	theme	Phylogenetic	400:411	arg1	analyses					413:420	Phylogenetic analyses	400:420	Phylogenetic analyses based on 16S rRNA genes	400:444	Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-CFT486T associated with Aeromicrobium panacisoli (97.0 % sequence identity), Aeromicrobium lacus (97.0 %), Aeromicrobium erythreum (96.8 %) and Aeromicrobium alkaliterrae (96.8 %), and lower sequence similarity values to other species.
33400640	11	74	theme	terrae	1653:1658	arg1	sp					1660:1661	the name Aeromicrobium terrae sp	1630:1661	the name Aeromicrobium terrae sp	1630:1661	Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence and ANI analyses, strain CC-CFT486T is proposed to represent a novel Aeromicrobium species, for which the name Aeromicrobium terrae sp.
33400640	8	75	theme	polar	979:983	arg1	profile					991:997	The polar lipid profile	975:997	The polar lipid profile	975:997	The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, two unidentified aminophospholipids and three unknown phospholipids.
33400640	4	76	theme	1 	390:391	arg1	%					392:392	%	392:392	%	392:392	Optimal growth occurred at 30 °С, pH 8 and 1 % NaCl.
33400640	7	77	theme	Strain	869:874	arg1	CC-CFT486T					876:885	Strain CC-CFT486T	869:885	Strain CC-CFT486T	869:885	Strain CC-CFT486T contained C16 : 0, C17 : 0, C17 : 1 ω8c and C18 : 1 ω9c as the predominant fatty acids.
33400640	12	78	theme	type	1670:1673	arg1	33499T					1709:1714	type strain CC-CFT486T=BCRC 81217T=JCM 33499T	1670:1714	type strain CC-CFT486T=BCRC 81217T=JCM 33499T	1670:1714	nov. (type strain CC-CFT486T=BCRC 81217T=JCM 33499T).
33400640	7	79	contain	contained	887:895	arg2	ω8c					923:925	ω8c	923:925	ω8c	923:925	Strain CC-CFT486T contained C16 : 0, C17 : 0, C17 : 1 ω8c and C18 : 1 ω9c as the predominant fatty acids.
33400640	7	79	contain	contained	887:895	arg2	C16 					897:900	C16 	897:900	C16 	897:900	Strain CC-CFT486T contained C16 : 0, C17 : 0, C17 : 1 ω8c and C18 : 1 ω9c as the predominant fatty acids.
33400640	7	79	contain	contained	887:895	arg1	CC-CFT486T					876:885	Strain CC-CFT486T	869:885	Strain CC-CFT486T	869:885	Strain CC-CFT486T contained C16 : 0, C17 : 0, C17 : 1 ω8c and C18 : 1 ω9c as the predominant fatty acids.
33400640	7	79	contain	contained	887:895	arg2	acids					968:972	the predominant fatty acids	946:972	the predominant fatty acids	946:972	Strain CC-CFT486T contained C16 : 0, C17 : 0, C17 : 1 ω8c and C18 : 1 ω9c as the predominant fatty acids.
33400640	7	79	contain	contained	887:895	arg2	C17 					915:918	C17 	915:918	C17 	915:918	Strain CC-CFT486T contained C16 : 0, C17 : 0, C17 : 1 ω8c and C18 : 1 ω9c as the predominant fatty acids.
33400640	7	79	contain	contained	887:895	arg2	C17 					906:909	C17 	906:909	C17 	906:909	Strain CC-CFT486T contained C16 : 0, C17 : 0, C17 : 1 ω8c and C18 : 1 ω9c as the predominant fatty acids.
33400640	7	79	contain	contained	887:895	arg2	ω9c					939:941	ω9c	939:941	ω9c	939:941	Strain CC-CFT486T contained C16 : 0, C17 : 0, C17 : 1 ω8c and C18 : 1 ω9c as the predominant fatty acids.
33400640	6	80	theme	type	828:831	arg1	strains					833:839	the type strains	824:839	the type strains of the genus Aeromicrobium	824:866	Average nucleotide identity (ANI) values were 70.6-77.8 % (n=11) compared within the type strains of the genus Aeromicrobium.
33400640	11	81	theme	name	1634:1637	arg1	sp					1660:1661	the name Aeromicrobium terrae sp	1630:1661	the name Aeromicrobium terrae sp	1630:1661	Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence and ANI analyses, strain CC-CFT486T is proposed to represent a novel Aeromicrobium species, for which the name Aeromicrobium terrae sp.
33400640	11	82	theme	rRNA	1509:1512	arg1	sequence					1519:1526	comparative 16S rRNA gene sequence	1493:1526	comparative 16S rRNA gene sequence	1493:1526	Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence and ANI analyses, strain CC-CFT486T is proposed to represent a novel Aeromicrobium species, for which the name Aeromicrobium terrae sp.
33400640	5	83	theme	other	728:732	arg1	species					734:740	other species	728:740	other species	728:740	Phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by strain CC-CFT486T associated with Aeromicrobium panacisoli (97.0 % sequence identity), Aeromicrobium lacus (97.0 %), Aeromicrobium erythreum (96.8 %) and Aeromicrobium alkaliterrae (96.8 %), and lower sequence similarity values to other species.
33400640	9	84	theme	major	1275:1279	arg1	spermidine					1295:1304	the major polyamine was spermidine	1271:1304	the major polyamine was spermidine	1271:1304	The cell wall peptidoglycan of strains CC-CFT486T contained ll-diaminopimelic acid (ll-DAP) and the major polyamine was spermidine.
33141838	4	0	theme	fecal	749:753	arg1	IgA					773:775	IgA	773:775	IgA	773:775	Fecal samples collected after 3 and 6 weeks' feeding were assayed for stool firmness score, short-chain fatty acids, ammonia, and changes to the global metabolome and microbiome; fecal immunoglobulin A (IgA) was analyzed at week 6.
33141838	4	0	theme	fecal	749:753	arg1	A					770:770	fecal immunoglobulin A	749:770	fecal immunoglobulin A (IgA)	749:776	Fecal samples collected after 3 and 6 weeks' feeding were assayed for stool firmness score, short-chain fatty acids, ammonia, and changes to the global metabolome and microbiome; fecal immunoglobulin A (IgA) was analyzed at week 6.
33141838	9	1	theme	precursors	1700:1709	arg1	members					1651:1657	reduced members	1643:1657	reduced members of NADH-coupled redox congeners and NADH precursors	1643:1709	Consumption of HRS food increased concentrations of the ketone body 3-hydroxybutyrate in feces and elevated concentrations of reduced members of NADH-coupled redox congeners and NADH precursors.
33141838	8	2	theme	HRS	1420:1422	arg1	food					1424:1427	HRS food	1420:1427	HRS food relative to those fed the LS food	1420:1461	RS consumption altered 47% of the fecal metabolome; RS-derived sugars and metabolites associated with greater gut health, including indoles and polyamines, increased in the cats consuming HRS food relative to those fed the LS food, while endocannabinoid N-acylethanolamines decreased.
33141838	10	3	theme	microbiome	1719:1728	arg1	genus-level					1730:1740	the microbiome genus-level	1715:1740	the microbiome genus-level	1715:1740	At the microbiome genus-level, 21% of operational taxonomic units were significantly different between food types; many involved taxa with known saccharolytic or proteolytic proclivities.
33141838	2	4	theme	RS	359:360	arg1	intake					362:367	RS intake	359:367	RS intake	359:367	The degree to which cats manifest beneficial changes in response to RS intake was examined.
33141838	1	5	theme	gut	255:257	arg1	metabolism					279:288	gut microbiome-mediated metabolism	255:288	gut microbiome-mediated metabolism	255:288	Dietary digestion-resistant starch (RS) provides health benefits to the host via gut microbiome-mediated metabolism.
33141838	8	6	theme	relative	1429:1436	arg1	food					1424:1427	HRS food	1420:1427	HRS food relative to those fed the LS food	1420:1461	RS consumption altered 47% of the fecal metabolome; RS-derived sugars and metabolites associated with greater gut health, including indoles and polyamines, increased in the cats consuming HRS food relative to those fed the LS food, while endocannabinoid N-acylethanolamines decreased.
33141838	10	7	theme	operational	1750:1760	arg1	units					1772:1776	operational taxonomic units	1750:1776	operational taxonomic units	1750:1776	At the microbiome genus-level, 21% of operational taxonomic units were significantly different between food types; many involved taxa with known saccharolytic or proteolytic proclivities.
33141838	6	8	theme	fecal	1083:1087	arg1	ammonia					1089:1095	fecal ammonia	1083:1095	fecal ammonia	1083:1095	In cats consuming HRS food, concentrations of fecal butyrate and the straight chain:branched chain fatty acid ratio were significantly greater in feces at both weeks 3 and 6, while fecal ammonia was reduced at week 6 relative to feces from LRS-fed cats.
33141838	9	9	from	concentrations	1625:1638	arg1	feces					1606:1610	feces	1606:1610	feces	1606:1610	Consumption of HRS food increased concentrations of the ketone body 3-hydroxybutyrate in feces and elevated concentrations of reduced members of NADH-coupled redox congeners and NADH precursors.
33141838	7	10	theme	HRS	1222:1224	arg1	food					1226:1229	HRS food	1222:1229	HRS food	1222:1229	Fecal IgA concentrations were significantly higher at week 6 with HRS food.
33141838	4	11	theme	Fecal	570:574	arg1	samples					576:582	Fecal samples	570:582	Fecal samples collected after 3 and 6 weeks' feeding	570:621	Fecal samples collected after 3 and 6 weeks' feeding were assayed for stool firmness score, short-chain fatty acids, ammonia, and changes to the global metabolome and microbiome; fecal immunoglobulin A (IgA) was analyzed at week 6.
33141838	3	12	theme	=	483:483	arg1	n					481:481	n = 19	481:486	n = 19	481:486	Healthy cats (N = 36) were fed identically formulated foods processed under high (n = 17) or low (n = 19) shear extrusion conditions (low and high RS levels [LRS and HRS], respectively).
33141838	1	13	theme	digestion-resistant	182:200	arg1	starch					202:207	Dietary digestion-resistant starch	174:207	Dietary digestion-resistant starch (RS)	174:212	Dietary digestion-resistant starch (RS) provides health benefits to the host via gut microbiome-mediated metabolism.
33141838	1	13	theme	digestion-resistant	182:200	arg1	RS					210:211	RS	210:211	RS	210:211	Dietary digestion-resistant starch (RS) provides health benefits to the host via gut microbiome-mediated metabolism.
33141838	6	14	theme	chain	995:999	arg1	acid					1007:1010	branched chain fatty acid	986:1010	the straight chain:branched chain fatty acid ratio	967:1016	In cats consuming HRS food, concentrations of fecal butyrate and the straight chain:branched chain fatty acid ratio were significantly greater in feces at both weeks 3 and 6, while fecal ammonia was reduced at week 6 relative to feces from LRS-fed cats.
33141838	10	15	theme	units	1772:1776	arg1	%					1745:1745	21%	1743:1745	21% of operational taxonomic units	1743:1776	At the microbiome genus-level, 21% of operational taxonomic units were significantly different between food types; many involved taxa with known saccharolytic or proteolytic proclivities.
33141838	10	15	theme	units	1772:1776	arg1	units					1772:1776	operational taxonomic units	1750:1776	operational taxonomic units	1750:1776	At the microbiome genus-level, 21% of operational taxonomic units were significantly different between food types; many involved taxa with known saccharolytic or proteolytic proclivities.
33141838	3	16	theme	extrusion	495:503	arg1	conditions					505:514	high (n = 17) or low (n = 19) shear extrusion conditions	459:514	high (n = 17) or low (n = 19) shear extrusion conditions (low and high RS levels [LRS and HRS], respectively)	459:567	Healthy cats (N = 36) were fed identically formulated foods processed under high (n = 17) or low (n = 19) shear extrusion conditions (low and high RS levels [LRS and HRS], respectively).
33141838	0	17	theme	immunoglobulin	145:158	arg1	A					160:160	immunoglobulin A	145:160	immunoglobulin A in the cat	145:171	Dietary resistant starch preserved through mild extrusion of grain alters fecal microbiome metabolism of dietary macronutrients while increasing immunoglobulin A in the cat.
33141838	3	18	theme	RS	530:531	arg1	[LRS					540:543	low and high RS levels [LRS	517:543	low and high RS levels [LRS	517:543	Healthy cats (N = 36) were fed identically formulated foods processed under high (n = 17) or low (n = 19) shear extrusion conditions (low and high RS levels [LRS and HRS], respectively).
33141838	1	19	theme	health	223:228	arg1	benefits					230:237	health benefits	223:237	health benefits	223:237	Dietary digestion-resistant starch (RS) provides health benefits to the host via gut microbiome-mediated metabolism.
33141838	11	20	theme	taxa	1911:1914	arg1	richness					1916:1923	Microbiome taxa richness	1900:1923	Microbiome taxa richness	1900:1923	Microbiome taxa richness and Shannon and Simpson alpha diversity were significantly higher in the HRS group at both weeks.
33141838	9	21	theme	HRS	1532:1534	arg1	food					1536:1539	HRS food	1532:1539	HRS food	1532:1539	Consumption of HRS food increased concentrations of the ketone body 3-hydroxybutyrate in feces and elevated concentrations of reduced members of NADH-coupled redox congeners and NADH precursors.
33141838	7	22	theme	Fecal	1156:1160	arg1	concentrations					1166:1179	Fecal IgA concentrations	1156:1179	Fecal IgA concentrations	1156:1179	Fecal IgA concentrations were significantly higher at week 6 with HRS food.
33141838	12	23	theme	feline	2044:2049	arg1	consumption					2051:2061	feline consumption	2044:2061	feline consumption of grain-derived RS	2044:2081	These data show that feline consumption of grain-derived RS produces potentially beneficial shifts in microbiota-mediated metabolism and increases IgA production.
33141838	12	24	link	grain-derived	2066:2078	arg1	RS					2080:2081	grain-derived RS	2066:2081	grain-derived RS	2066:2081	These data show that feline consumption of grain-derived RS produces potentially beneficial shifts in microbiota-mediated metabolism and increases IgA production.
33141838	8	25	theme	fecal	1266:1270	arg1	metabolome					1272:1281	the fecal metabolome	1262:1281	the fecal metabolome	1262:1281	RS consumption altered 47% of the fecal metabolome; RS-derived sugars and metabolites associated with greater gut health, including indoles and polyamines, increased in the cats consuming HRS food relative to those fed the LS food, while endocannabinoid N-acylethanolamines decreased.
33141838	10	26	theme	many	1827:1830	arg1	taxa					1841:1844	many involved taxa	1827:1844	food types; many involved taxa with known saccharolytic or proteolytic proclivities	1815:1897	At the microbiome genus-level, 21% of operational taxonomic units were significantly different between food types; many involved taxa with known saccharolytic or proteolytic proclivities.
33141838	0	27	theme	grain	61:65	arg1	extrusion					48:56	mild extrusion	43:56	mild extrusion of grain	43:65	Dietary resistant starch preserved through mild extrusion of grain alters fecal microbiome metabolism of dietary macronutrients while increasing immunoglobulin A in the cat.
33141838	11	28	theme	HRS	1998:2000	arg1	group					2002:2006	the HRS group	1994:2006	the HRS group	1994:2006	Microbiome taxa richness and Shannon and Simpson alpha diversity were significantly higher in the HRS group at both weeks.
33141838	0	29	theme	fecal	74:78	arg1	metabolism					91:100	fecal microbiome metabolism	74:100	fecal microbiome metabolism of dietary macronutrients	74:126	Dietary resistant starch preserved through mild extrusion of grain alters fecal microbiome metabolism of dietary macronutrients while increasing immunoglobulin A in the cat.
33141838	11	30	theme	Simpson	1941:1947	arg1	diversity					1955:1963	Shannon and Simpson alpha diversity	1929:1963	Shannon and Simpson alpha diversity	1929:1963	Microbiome taxa richness and Shannon and Simpson alpha diversity were significantly higher in the HRS group at both weeks.
33141838	9	31	theme	ketone	1573:1578	arg1	3-hydroxybutyrate					1585:1601	the ketone body 3-hydroxybutyrate	1569:1601	the ketone body 3-hydroxybutyrate in feces	1569:1610	Consumption of HRS food increased concentrations of the ketone body 3-hydroxybutyrate in feces and elevated concentrations of reduced members of NADH-coupled redox congeners and NADH precursors.
33141838	2	32	dep	intake	362:367	arg1	response					347:354	response	347:354	response	347:354	The degree to which cats manifest beneficial changes in response to RS intake was examined.
33141838	10	33	theme	known	1851:1855	arg1	proclivities					1886:1897	known saccharolytic or proteolytic proclivities	1851:1897	known saccharolytic or proteolytic proclivities	1851:1897	At the microbiome genus-level, 21% of operational taxonomic units were significantly different between food types; many involved taxa with known saccharolytic or proteolytic proclivities.
33141838	9	34	theme	3-hydroxybutyrate	1585:1601	arg1	concentrations					1625:1638	elevated concentrations	1616:1638	elevated concentrations of reduced members of NADH-coupled redox congeners and NADH precursors	1616:1709	Consumption of HRS food increased concentrations of the ketone body 3-hydroxybutyrate in feces and elevated concentrations of reduced members of NADH-coupled redox congeners and NADH precursors.
33141838	9	34	theme	3-hydroxybutyrate	1585:1601	arg1	concentrations					1551:1564	concentrations	1551:1564	concentrations of the ketone body 3-hydroxybutyrate in feces	1551:1610	Consumption of HRS food increased concentrations of the ketone body 3-hydroxybutyrate in feces and elevated concentrations of reduced members of NADH-coupled redox congeners and NADH precursors.
33141838	4	35	theme	fatty	674:678	arg1	acids					680:684	short-chain fatty acids	662:684	short-chain fatty acids	662:684	Fecal samples collected after 3 and 6 weeks' feeding were assayed for stool firmness score, short-chain fatty acids, ammonia, and changes to the global metabolome and microbiome; fecal immunoglobulin A (IgA) was analyzed at week 6.
33141838	11	36	theme	alpha	1949:1953	arg1	diversity					1955:1963	Shannon and Simpson alpha diversity	1929:1963	Shannon and Simpson alpha diversity	1929:1963	Microbiome taxa richness and Shannon and Simpson alpha diversity were significantly higher in the HRS group at both weeks.
33141838	0	37	theme	dietary	105:111	arg1	macronutrients					113:126	dietary macronutrients	105:126	dietary macronutrients	105:126	Dietary resistant starch preserved through mild extrusion of grain alters fecal microbiome metabolism of dietary macronutrients while increasing immunoglobulin A in the cat.
33141838	6	38	theme	HRS	920:922	arg1	food					924:927	HRS food	920:927	HRS food	920:927	In cats consuming HRS food, concentrations of fecal butyrate and the straight chain:branched chain fatty acid ratio were significantly greater in feces at both weeks 3 and 6, while fecal ammonia was reduced at week 6 relative to feces from LRS-fed cats.
33141838	12	39	theme	beneficial	2104:2113	arg1	shifts					2115:2120	potentially beneficial shifts	2092:2120	potentially beneficial shifts in microbiota-mediated metabolism	2092:2154	These data show that feline consumption of grain-derived RS produces potentially beneficial shifts in microbiota-mediated metabolism and increases IgA production.
33141838	0	40	theme	Dietary	0:6	arg1	starch					18:23	Dietary resistant starch	0:23	Dietary resistant starch preserved through mild extrusion of grain	0:65	Dietary resistant starch preserved through mild extrusion of grain alters fecal microbiome metabolism of dietary macronutrients while increasing immunoglobulin A in the cat.
33141838	5	41	theme	Few	802:804	arg1	differences					806:816	Few differences	802:816	Few differences	802:816	Few differences were seen in proximate analyses of the foods; stool firmness scores did not differ.
33141838	9	42	theme	elevated	1616:1623	arg1	concentrations					1625:1638	elevated concentrations	1616:1638	elevated concentrations of reduced members of NADH-coupled redox congeners and NADH precursors	1616:1709	Consumption of HRS food increased concentrations of the ketone body 3-hydroxybutyrate in feces and elevated concentrations of reduced members of NADH-coupled redox congeners and NADH precursors.
33141838	8	43	theme	gut	1342:1344	arg1	health					1346:1351	greater gut health	1334:1351	greater gut health	1334:1351	RS consumption altered 47% of the fecal metabolome; RS-derived sugars and metabolites associated with greater gut health, including indoles and polyamines, increased in the cats consuming HRS food relative to those fed the LS food, while endocannabinoid N-acylethanolamines decreased.
33141838	4	44	theme	firmness	646:653	arg1	score					655:659	stool firmness score	640:659	stool firmness score	640:659	Fecal samples collected after 3 and 6 weeks' feeding were assayed for stool firmness score, short-chain fatty acids, ammonia, and changes to the global metabolome and microbiome; fecal immunoglobulin A (IgA) was analyzed at week 6.
33141838	10	45	with	taxa	1841:1844	arg1	proclivities					1886:1897	known saccharolytic or proteolytic proclivities	1851:1897	known saccharolytic or proteolytic proclivities	1851:1897	At the microbiome genus-level, 21% of operational taxonomic units were significantly different between food types; many involved taxa with known saccharolytic or proteolytic proclivities.
33141838	9	46	from	3-hydroxybutyrate	1585:1601	arg1	feces					1606:1610	feces	1606:1610	feces	1606:1610	Consumption of HRS food increased concentrations of the ketone body 3-hydroxybutyrate in feces and elevated concentrations of reduced members of NADH-coupled redox congeners and NADH precursors.
33141838	5	47	theme	firmness	870:877	arg1	scores					879:884	stool firmness scores	864:884	stool firmness scores	864:884	Few differences were seen in proximate analyses of the foods; stool firmness scores did not differ.
33141838	9	48	theme	members	1651:1657	arg1	concentrations					1625:1638	elevated concentrations	1616:1638	elevated concentrations of reduced members of NADH-coupled redox congeners and NADH precursors	1616:1709	Consumption of HRS food increased concentrations of the ketone body 3-hydroxybutyrate in feces and elevated concentrations of reduced members of NADH-coupled redox congeners and NADH precursors.
33141838	9	48	theme	members	1651:1657	arg1	concentrations					1551:1564	concentrations	1551:1564	concentrations of the ketone body 3-hydroxybutyrate in feces	1551:1610	Consumption of HRS food increased concentrations of the ketone body 3-hydroxybutyrate in feces and elevated concentrations of reduced members of NADH-coupled redox congeners and NADH precursors.
33141838	3	49	theme	=	467:467	arg1	n					465:465	n = 17	465:470	n = 17	465:470	Healthy cats (N = 36) were fed identically formulated foods processed under high (n = 17) or low (n = 19) shear extrusion conditions (low and high RS levels [LRS and HRS], respectively).
33141838	12	50	theme	microbiota-mediated	2125:2143	arg1	metabolism					2145:2154	microbiota-mediated metabolism	2125:2154	microbiota-mediated metabolism	2125:2154	These data show that feline consumption of grain-derived RS produces potentially beneficial shifts in microbiota-mediated metabolism and increases IgA production.
33141838	9	51	theme	NADH-coupled	1662:1673	arg1	congeners					1681:1689	NADH-coupled redox congeners	1662:1689	NADH-coupled redox congeners	1662:1689	Consumption of HRS food increased concentrations of the ketone body 3-hydroxybutyrate in feces and elevated concentrations of reduced members of NADH-coupled redox congeners and NADH precursors.
33141838	6	52	theme	butyrate	954:961	arg1	concentrations					930:943	concentrations	930:943	concentrations of fecal butyrate and the straight chain:branched chain fatty acid ratio	930:1016	In cats consuming HRS food, concentrations of fecal butyrate and the straight chain:branched chain fatty acid ratio were significantly greater in feces at both weeks 3 and 6, while fecal ammonia was reduced at week 6 relative to feces from LRS-fed cats.
33141838	9	53	theme	congeners	1681:1689	arg1	members					1651:1657	reduced members	1643:1657	reduced members of NADH-coupled redox congeners and NADH precursors	1643:1709	Consumption of HRS food increased concentrations of the ketone body 3-hydroxybutyrate in feces and elevated concentrations of reduced members of NADH-coupled redox congeners and NADH precursors.
33141838	8	54	theme	RS	1232:1233	arg1	consumption					1235:1245	RS consumption	1232:1245	RS consumption	1232:1245	RS consumption altered 47% of the fecal metabolome; RS-derived sugars and metabolites associated with greater gut health, including indoles and polyamines, increased in the cats consuming HRS food relative to those fed the LS food, while endocannabinoid N-acylethanolamines decreased.
33141838	3	55	theme	low	476:478	arg1	conditions					505:514	high (n = 17) or low (n = 19) shear extrusion conditions	459:514	high (n = 17) or low (n = 19) shear extrusion conditions (low and high RS levels [LRS and HRS], respectively)	459:567	Healthy cats (N = 36) were fed identically formulated foods processed under high (n = 17) or low (n = 19) shear extrusion conditions (low and high RS levels [LRS and HRS], respectively).
33141838	3	56	dep	high	459:462	arg1	n					465:465	n = 17	465:470	n = 17	465:470	Healthy cats (N = 36) were fed identically formulated foods processed under high (n = 17) or low (n = 19) shear extrusion conditions (low and high RS levels [LRS and HRS], respectively).
33141838	9	57	theme	NADH	1695:1698	arg1	precursors					1700:1709	NADH precursors	1695:1709	NADH precursors	1695:1709	Consumption of HRS food increased concentrations of the ketone body 3-hydroxybutyrate in feces and elevated concentrations of reduced members of NADH-coupled redox congeners and NADH precursors.
33141838	8	58	theme	LS	1455:1456	arg1	food					1458:1461	the LS food	1451:1461	the LS food	1451:1461	RS consumption altered 47% of the fecal metabolome; RS-derived sugars and metabolites associated with greater gut health, including indoles and polyamines, increased in the cats consuming HRS food relative to those fed the LS food, while endocannabinoid N-acylethanolamines decreased.
33141838	1	59	theme	microbiome-mediated	259:277	arg1	metabolism					279:288	gut microbiome-mediated metabolism	255:288	gut microbiome-mediated metabolism	255:288	Dietary digestion-resistant starch (RS) provides health benefits to the host via gut microbiome-mediated metabolism.
33141838	4	60	theme	immunoglobulin	755:768	arg1	IgA					773:775	IgA	773:775	IgA	773:775	Fecal samples collected after 3 and 6 weeks' feeding were assayed for stool firmness score, short-chain fatty acids, ammonia, and changes to the global metabolome and microbiome; fecal immunoglobulin A (IgA) was analyzed at week 6.
33141838	4	60	theme	immunoglobulin	755:768	arg1	A					770:770	fecal immunoglobulin A	749:770	fecal immunoglobulin A (IgA)	749:776	Fecal samples collected after 3 and 6 weeks' feeding were assayed for stool firmness score, short-chain fatty acids, ammonia, and changes to the global metabolome and microbiome; fecal immunoglobulin A (IgA) was analyzed at week 6.
33141838	6	61	theme	ratio	1012:1016	arg1	concentrations					930:943	concentrations	930:943	concentrations of fecal butyrate and the straight chain:branched chain fatty acid ratio	930:1016	In cats consuming HRS food, concentrations of fecal butyrate and the straight chain:branched chain fatty acid ratio were significantly greater in feces at both weeks 3 and 6, while fecal ammonia was reduced at week 6 relative to feces from LRS-fed cats.
33141838	8	62	theme	endocannabinoid	1470:1484	arg1	N-acylethanolamines					1486:1504	endocannabinoid N-acylethanolamines	1470:1504	endocannabinoid N-acylethanolamines	1470:1504	RS consumption altered 47% of the fecal metabolome; RS-derived sugars and metabolites associated with greater gut health, including indoles and polyamines, increased in the cats consuming HRS food relative to those fed the LS food, while endocannabinoid N-acylethanolamines decreased.
33141838	1	63	theme	Dietary	174:180	arg1	starch					202:207	Dietary digestion-resistant starch	174:207	Dietary digestion-resistant starch (RS)	174:212	Dietary digestion-resistant starch (RS) provides health benefits to the host via gut microbiome-mediated metabolism.
33141838	1	63	theme	Dietary	174:180	arg1	RS					210:211	RS	210:211	RS	210:211	Dietary digestion-resistant starch (RS) provides health benefits to the host via gut microbiome-mediated metabolism.
33141838	9	64	from	feces	1606:1610	arg1	concentrations					1625:1638	elevated concentrations	1616:1638	elevated concentrations of reduced members of NADH-coupled redox congeners and NADH precursors	1616:1709	Consumption of HRS food increased concentrations of the ketone body 3-hydroxybutyrate in feces and elevated concentrations of reduced members of NADH-coupled redox congeners and NADH precursors.
33141838	9	64	from	feces	1606:1610	arg1	concentrations					1551:1564	concentrations	1551:1564	concentrations of the ketone body 3-hydroxybutyrate in feces	1551:1610	Consumption of HRS food increased concentrations of the ketone body 3-hydroxybutyrate in feces and elevated concentrations of reduced members of NADH-coupled redox congeners and NADH precursors.
33141838	4	65	theme	global	715:720	arg1	metabolome					722:731	the global metabolome	711:731	the global metabolome	711:731	Fecal samples collected after 3 and 6 weeks' feeding were assayed for stool firmness score, short-chain fatty acids, ammonia, and changes to the global metabolome and microbiome; fecal immunoglobulin A (IgA) was analyzed at week 6.
33141838	6	66	theme	fatty	1001:1005	arg1	acid					1007:1010	branched chain fatty acid	986:1010	the straight chain:branched chain fatty acid ratio	967:1016	In cats consuming HRS food, concentrations of fecal butyrate and the straight chain:branched chain fatty acid ratio were significantly greater in feces at both weeks 3 and 6, while fecal ammonia was reduced at week 6 relative to feces from LRS-fed cats.
33141838	6	67	dep	chain	980:984	arg1	acid					1007:1010	branched chain fatty acid	986:1010	the straight chain:branched chain fatty acid ratio	967:1016	In cats consuming HRS food, concentrations of fecal butyrate and the straight chain:branched chain fatty acid ratio were significantly greater in feces at both weeks 3 and 6, while fecal ammonia was reduced at week 6 relative to feces from LRS-fed cats.
33141838	2	68	theme	beneficial	325:334	arg1	changes					336:342	beneficial changes	325:342	beneficial changes	325:342	The degree to which cats manifest beneficial changes in response to RS intake was examined.
33141838	6	69	theme	branched	986:993	arg1	acid					1007:1010	branched chain fatty acid	986:1010	the straight chain:branched chain fatty acid ratio	967:1016	In cats consuming HRS food, concentrations of fecal butyrate and the straight chain:branched chain fatty acid ratio were significantly greater in feces at both weeks 3 and 6, while fecal ammonia was reduced at week 6 relative to feces from LRS-fed cats.
33141838	6	70	theme	LRS-fed	1142:1148	arg1	cats					1150:1153	LRS-fed cats	1142:1153	LRS-fed cats	1142:1153	In cats consuming HRS food, concentrations of fecal butyrate and the straight chain:branched chain fatty acid ratio were significantly greater in feces at both weeks 3 and 6, while fecal ammonia was reduced at week 6 relative to feces from LRS-fed cats.
33141838	3	71	theme	shear	489:493	arg1	conditions					505:514	high (n = 17) or low (n = 19) shear extrusion conditions	459:514	high (n = 17) or low (n = 19) shear extrusion conditions (low and high RS levels [LRS and HRS], respectively)	459:567	Healthy cats (N = 36) were fed identically formulated foods processed under high (n = 17) or low (n = 19) shear extrusion conditions (low and high RS levels [LRS and HRS], respectively).
33141838	10	72	from	genus-level	1730:1740	arg1	different					1797:1805	different	1797:1805	different	1797:1805	At the microbiome genus-level, 21% of operational taxonomic units were significantly different between food types; many involved taxa with known saccharolytic or proteolytic proclivities.
33141838	11	73	theme	Microbiome	1900:1909	arg1	richness					1916:1923	Microbiome taxa richness	1900:1923	Microbiome taxa richness	1900:1923	Microbiome taxa richness and Shannon and Simpson alpha diversity were significantly higher in the HRS group at both weeks.
33141838	6	74	theme	chain	980:984	arg1	ratio					1012:1016	the straight chain:branched chain fatty acid ratio	967:1016	the straight chain:branched chain fatty acid ratio	967:1016	In cats consuming HRS food, concentrations of fecal butyrate and the straight chain:branched chain fatty acid ratio were significantly greater in feces at both weeks 3 and 6, while fecal ammonia was reduced at week 6 relative to feces from LRS-fed cats.
33141838	10	75	theme	taxonomic	1762:1770	arg1	units					1772:1776	operational taxonomic units	1750:1776	operational taxonomic units	1750:1776	At the microbiome genus-level, 21% of operational taxonomic units were significantly different between food types; many involved taxa with known saccharolytic or proteolytic proclivities.
33141838	3	76	theme	high	525:528	arg1	[LRS					540:543	low and high RS levels [LRS	517:543	low and high RS levels [LRS	517:543	Healthy cats (N = 36) were fed identically formulated foods processed under high (n = 17) or low (n = 19) shear extrusion conditions (low and high RS levels [LRS and HRS], respectively).
33141838	10	77	theme	food	1815:1818	arg1	types					1820:1824	food types	1815:1824	food types; many involved taxa with known saccharolytic or proteolytic proclivities	1815:1897	At the microbiome genus-level, 21% of operational taxonomic units were significantly different between food types; many involved taxa with known saccharolytic or proteolytic proclivities.
33141838	3	78	theme	low	517:519	arg1	[LRS					540:543	low and high RS levels [LRS	517:543	low and high RS levels [LRS	517:543	Healthy cats (N = 36) were fed identically formulated foods processed under high (n = 17) or low (n = 19) shear extrusion conditions (low and high RS levels [LRS and HRS], respectively).
33141838	11	79	theme	Shannon	1929:1935	arg1	diversity					1955:1963	Shannon and Simpson alpha diversity	1929:1963	Shannon and Simpson alpha diversity	1929:1963	Microbiome taxa richness and Shannon and Simpson alpha diversity were significantly higher in the HRS group at both weeks.
33141838	12	80	theme	IgA	2170:2172	arg1	production					2174:2183	IgA production	2170:2183	IgA production	2170:2183	These data show that feline consumption of grain-derived RS produces potentially beneficial shifts in microbiota-mediated metabolism and increases IgA production.
33141838	9	81	theme	food	1536:1539	arg1	Consumption					1517:1527	Consumption	1517:1527	Consumption of HRS food	1517:1539	Consumption of HRS food increased concentrations of the ketone body 3-hydroxybutyrate in feces and elevated concentrations of reduced members of NADH-coupled redox congeners and NADH precursors.
33141838	8	82	theme	metabolome	1272:1281	arg1	%					1257:1257	47%	1255:1257	47% of the fecal metabolome	1255:1281	RS consumption altered 47% of the fecal metabolome; RS-derived sugars and metabolites associated with greater gut health, including indoles and polyamines, increased in the cats consuming HRS food relative to those fed the LS food, while endocannabinoid N-acylethanolamines decreased.
33141838	8	82	theme	metabolome	1272:1281	arg1	metabolome					1272:1281	the fecal metabolome	1262:1281	the fecal metabolome	1262:1281	RS consumption altered 47% of the fecal metabolome; RS-derived sugars and metabolites associated with greater gut health, including indoles and polyamines, increased in the cats consuming HRS food relative to those fed the LS food, while endocannabinoid N-acylethanolamines decreased.
33141838	7	83	theme	IgA	1162:1164	arg1	concentrations					1166:1179	Fecal IgA concentrations	1156:1179	Fecal IgA concentrations	1156:1179	Fecal IgA concentrations were significantly higher at week 6 with HRS food.
33141838	10	84	theme	involved	1832:1839	arg1	taxa					1841:1844	many involved taxa	1827:1844	food types; many involved taxa with known saccharolytic or proteolytic proclivities	1815:1897	At the microbiome genus-level, 21% of operational taxonomic units were significantly different between food types; many involved taxa with known saccharolytic or proteolytic proclivities.
33141838	3	85	theme	levels	533:538	arg1	[LRS					540:543	low and high RS levels [LRS	517:543	low and high RS levels [LRS	517:543	Healthy cats (N = 36) were fed identically formulated foods processed under high (n = 17) or low (n = 19) shear extrusion conditions (low and high RS levels [LRS and HRS], respectively).
33141838	8	86	theme	RS-derived	1284:1293	arg1	sugars					1295:1300	RS-derived sugars	1284:1300	RS-derived sugars	1284:1300	RS consumption altered 47% of the fecal metabolome; RS-derived sugars and metabolites associated with greater gut health, including indoles and polyamines, increased in the cats consuming HRS food relative to those fed the LS food, while endocannabinoid N-acylethanolamines decreased.
33141838	0	87	theme	microbiome	80:89	arg1	metabolism					91:100	fecal microbiome metabolism	74:100	fecal microbiome metabolism of dietary macronutrients	74:126	Dietary resistant starch preserved through mild extrusion of grain alters fecal microbiome metabolism of dietary macronutrients while increasing immunoglobulin A in the cat.
33141838	3	88	dep	conditions	505:514	arg1	[LRS					540:543	low and high RS levels [LRS	517:543	low and high RS levels [LRS	517:543	Healthy cats (N = 36) were fed identically formulated foods processed under high (n = 17) or low (n = 19) shear extrusion conditions (low and high RS levels [LRS and HRS], respectively).
33141838	3	88	dep	conditions	505:514	arg1	HRS					549:551	HRS	549:551	HRS	549:551	Healthy cats (N = 36) were fed identically formulated foods processed under high (n = 17) or low (n = 19) shear extrusion conditions (low and high RS levels [LRS and HRS], respectively).
33141838	10	89	dep	types	1820:1824	arg1	taxa					1841:1844	many involved taxa	1827:1844	food types; many involved taxa with known saccharolytic or proteolytic proclivities	1815:1897	At the microbiome genus-level, 21% of operational taxonomic units were significantly different between food types; many involved taxa with known saccharolytic or proteolytic proclivities.
33141838	6	90	dep	weeks	1062:1066	arg1	6					1074:1074	6	1074:1074	6	1074:1074	In cats consuming HRS food, concentrations of fecal butyrate and the straight chain:branched chain fatty acid ratio were significantly greater in feces at both weeks 3 and 6, while fecal ammonia was reduced at week 6 relative to feces from LRS-fed cats.
33141838	6	90	dep	weeks	1062:1066	arg1	3					1068:1068	3	1068:1068	3	1068:1068	In cats consuming HRS food, concentrations of fecal butyrate and the straight chain:branched chain fatty acid ratio were significantly greater in feces at both weeks 3 and 6, while fecal ammonia was reduced at week 6 relative to feces from LRS-fed cats.
33141838	10	91	theme	saccharolytic	1857:1869	arg1	proclivities					1886:1897	known saccharolytic or proteolytic proclivities	1851:1897	known saccharolytic or proteolytic proclivities	1851:1897	At the microbiome genus-level, 21% of operational taxonomic units were significantly different between food types; many involved taxa with known saccharolytic or proteolytic proclivities.
33141838	12	92	from	shifts	2115:2120	arg1	metabolism					2145:2154	microbiota-mediated metabolism	2125:2154	microbiota-mediated metabolism	2125:2154	These data show that feline consumption of grain-derived RS produces potentially beneficial shifts in microbiota-mediated metabolism and increases IgA production.
33141838	9	93	theme	body	1580:1583	arg1	3-hydroxybutyrate					1585:1601	the ketone body 3-hydroxybutyrate	1569:1601	the ketone body 3-hydroxybutyrate in feces	1569:1610	Consumption of HRS food increased concentrations of the ketone body 3-hydroxybutyrate in feces and elevated concentrations of reduced members of NADH-coupled redox congeners and NADH precursors.
33141838	0	94	theme	macronutrients	113:126	arg1	metabolism					91:100	fecal microbiome metabolism	74:100	fecal microbiome metabolism of dietary macronutrients	74:126	Dietary resistant starch preserved through mild extrusion of grain alters fecal microbiome metabolism of dietary macronutrients while increasing immunoglobulin A in the cat.
33141838	6	95	from	cats	1150:1153	arg1	feces					1131:1135	feces	1131:1135	feces from LRS-fed cats	1131:1153	In cats consuming HRS food, concentrations of fecal butyrate and the straight chain:branched chain fatty acid ratio were significantly greater in feces at both weeks 3 and 6, while fecal ammonia was reduced at week 6 relative to feces from LRS-fed cats.
33141838	5	96	theme	proximate	831:839	arg1	analyses					841:848	proximate analyses	831:848	proximate analyses of the foods	831:861	Few differences were seen in proximate analyses of the foods; stool firmness scores did not differ.
33141838	8	97	theme	greater	1334:1340	arg1	health					1346:1351	greater gut health	1334:1351	greater gut health	1334:1351	RS consumption altered 47% of the fecal metabolome; RS-derived sugars and metabolites associated with greater gut health, including indoles and polyamines, increased in the cats consuming HRS food relative to those fed the LS food, while endocannabinoid N-acylethanolamines decreased.
33141838	4	98	theme	short-chain	662:672	arg1	acids					680:684	short-chain fatty acids	662:684	short-chain fatty acids	662:684	Fecal samples collected after 3 and 6 weeks' feeding were assayed for stool firmness score, short-chain fatty acids, ammonia, and changes to the global metabolome and microbiome; fecal immunoglobulin A (IgA) was analyzed at week 6.
33141838	3	99	theme	formulated	426:435	arg1	foods					437:441	identically formulated foods	414:441	identically formulated foods processed under high (n = 17) or low (n = 19) shear extrusion conditions (low and high RS levels [LRS and HRS], respectively)	414:567	Healthy cats (N = 36) were fed identically formulated foods processed under high (n = 17) or low (n = 19) shear extrusion conditions (low and high RS levels [LRS and HRS], respectively).
33141838	0	100	theme	resistant	8:16	arg1	starch					18:23	Dietary resistant starch	0:23	Dietary resistant starch preserved through mild extrusion of grain	0:65	Dietary resistant starch preserved through mild extrusion of grain alters fecal microbiome metabolism of dietary macronutrients while increasing immunoglobulin A in the cat.
33141838	3	101	dep	low	476:478	arg1	n					481:481	n = 19	481:486	n = 19	481:486	Healthy cats (N = 36) were fed identically formulated foods processed under high (n = 17) or low (n = 19) shear extrusion conditions (low and high RS levels [LRS and HRS], respectively).
33141838	3	102	theme	Healthy	383:389	arg1	cats					391:394	Healthy cats	383:394	Healthy cats (N = 36)	383:403	Healthy cats (N = 36) were fed identically formulated foods processed under high (n = 17) or low (n = 19) shear extrusion conditions (low and high RS levels [LRS and HRS], respectively).
33141838	3	102	theme	Healthy	383:389	arg1	N					397:397	N = 36	397:402	N = 36	397:402	Healthy cats (N = 36) were fed identically formulated foods processed under high (n = 17) or low (n = 19) shear extrusion conditions (low and high RS levels [LRS and HRS], respectively).
33141838	12	103	theme	RS	2080:2081	arg1	consumption					2051:2061	feline consumption	2044:2061	feline consumption of grain-derived RS	2044:2081	These data show that feline consumption of grain-derived RS produces potentially beneficial shifts in microbiota-mediated metabolism and increases IgA production.
33141838	4	104	theme	stool	640:644	arg1	score					655:659	stool firmness score	640:659	stool firmness score	640:659	Fecal samples collected after 3 and 6 weeks' feeding were assayed for stool firmness score, short-chain fatty acids, ammonia, and changes to the global metabolome and microbiome; fecal immunoglobulin A (IgA) was analyzed at week 6.
33141838	3	105	theme	high	459:462	arg1	conditions					505:514	high (n = 17) or low (n = 19) shear extrusion conditions	459:514	high (n = 17) or low (n = 19) shear extrusion conditions (low and high RS levels [LRS and HRS], respectively)	459:567	Healthy cats (N = 36) were fed identically formulated foods processed under high (n = 17) or low (n = 19) shear extrusion conditions (low and high RS levels [LRS and HRS], respectively).
33141838	0	106	theme	mild	43:46	arg1	extrusion					48:56	mild extrusion	43:56	mild extrusion of grain	43:65	Dietary resistant starch preserved through mild extrusion of grain alters fecal microbiome metabolism of dietary macronutrients while increasing immunoglobulin A in the cat.
33141838	5	107	theme	stool	864:868	arg1	scores					879:884	stool firmness scores	864:884	stool firmness scores	864:884	Few differences were seen in proximate analyses of the foods; stool firmness scores did not differ.
33141838	9	108	theme	reduced	1643:1649	arg1	members					1651:1657	reduced members	1643:1657	reduced members of NADH-coupled redox congeners and NADH precursors	1643:1709	Consumption of HRS food increased concentrations of the ketone body 3-hydroxybutyrate in feces and elevated concentrations of reduced members of NADH-coupled redox congeners and NADH precursors.
33141838	6	109	theme	straight	971:978	arg1	chain					980:984	the straight chain	967:984	the straight chain:branched chain fatty acid ratio	967:1016	In cats consuming HRS food, concentrations of fecal butyrate and the straight chain:branched chain fatty acid ratio were significantly greater in feces at both weeks 3 and 6, while fecal ammonia was reduced at week 6 relative to feces from LRS-fed cats.
33141838	3	110	theme	=	399:399	arg1	cats					391:394	Healthy cats	383:394	Healthy cats (N = 36)	383:403	Healthy cats (N = 36) were fed identically formulated foods processed under high (n = 17) or low (n = 19) shear extrusion conditions (low and high RS levels [LRS and HRS], respectively).
33141838	3	110	theme	=	399:399	arg1	N					397:397	N = 36	397:402	N = 36	397:402	Healthy cats (N = 36) were fed identically formulated foods processed under high (n = 17) or low (n = 19) shear extrusion conditions (low and high RS levels [LRS and HRS], respectively).
33141838	8	111	link	RS-derived	1284:1293	arg1	sugars					1295:1300	RS-derived sugars	1284:1300	RS-derived sugars	1284:1300	RS consumption altered 47% of the fecal metabolome; RS-derived sugars and metabolites associated with greater gut health, including indoles and polyamines, increased in the cats consuming HRS food relative to those fed the LS food, while endocannabinoid N-acylethanolamines decreased.
33141838	9	112	theme	redox	1675:1679	arg1	congeners					1681:1689	NADH-coupled redox congeners	1662:1689	NADH-coupled redox congeners	1662:1689	Consumption of HRS food increased concentrations of the ketone body 3-hydroxybutyrate in feces and elevated concentrations of reduced members of NADH-coupled redox congeners and NADH precursors.
33141838	0	113	from	A	160:160	arg1	cat					169:171	the cat	165:171	the cat	165:171	Dietary resistant starch preserved through mild extrusion of grain alters fecal microbiome metabolism of dietary macronutrients while increasing immunoglobulin A in the cat.
33141838	12	114	theme	grain-derived	2066:2078	arg1	RS					2080:2081	grain-derived RS	2066:2081	grain-derived RS	2066:2081	These data show that feline consumption of grain-derived RS produces potentially beneficial shifts in microbiota-mediated metabolism and increases IgA production.
33141838	9	115	from	concentrations	1551:1564	arg1	feces					1606:1610	feces	1606:1610	feces	1606:1610	Consumption of HRS food increased concentrations of the ketone body 3-hydroxybutyrate in feces and elevated concentrations of reduced members of NADH-coupled redox congeners and NADH precursors.
33141838	5	116	theme	foods	857:861	arg1	analyses					841:848	proximate analyses	831:848	proximate analyses of the foods	831:861	Few differences were seen in proximate analyses of the foods; stool firmness scores did not differ.
33141838	6	117	theme	fecal	948:952	arg1	butyrate					954:961	fecal butyrate	948:961	fecal butyrate	948:961	In cats consuming HRS food, concentrations of fecal butyrate and the straight chain:branched chain fatty acid ratio were significantly greater in feces at both weeks 3 and 6, while fecal ammonia was reduced at week 6 relative to feces from LRS-fed cats.
33141838	10	118	theme	proteolytic	1874:1884	arg1	proclivities					1886:1897	known saccharolytic or proteolytic proclivities	1851:1897	known saccharolytic or proteolytic proclivities	1851:1897	At the microbiome genus-level, 21% of operational taxonomic units were significantly different between food types; many involved taxa with known saccharolytic or proteolytic proclivities.
32931405	13	0	theme	type	1551:1554	arg1	strain					1556:1561	The type strain	1547:1561	The type strain	1547:1561	The type strain is CFH S0501T (=DSM 29940T=BCRC 80809T).
32931405	13	0	theme	type	1551:1554	arg1	S0501T					1570:1575	CFH S0501T	1566:1575	CFH S0501T (=DSM 29940T=BCRC 80809T)	1566:1601	The type strain is CFH S0501T (=DSM 29940T=BCRC 80809T).
32931405	10	1	theme	%	1306:1306	arg1	%					1323:1323	% and DDH <25.00 %	1306:1323	% and DDH <25.00 %	1306:1323	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	2	2	from	River	281:285	arg1	Province					296:303	Province	296:303	Province	296:303	Strain CFH S0501T, a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella, was isolated from a sediment sample collected from the Yellow River in Henan Province, PR China.
32931405	4	3	theme	Brevibacillus	527:539	arg1	8445T					558:562	Brevibacillus centrosporus DSM 8445T	527:562	Brevibacillus centrosporus DSM 8445T	527:562	Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain belonged to the genus Brevibacillus and was closely related to Brevibacillus centrosporus DSM 8445T and Brevibacillus ginsengisoli Gsoil 3088T (with 96.8 and 96.7 % sequence similarity, respectively).
32931405	11	4	theme	strain	1422:1427	arg1	S0501T					1433:1438	strain CFH S0501T	1422:1438	strain CFH S0501T	1422:1438	Based on physiological properties, chemotaxonomic characteristics and low ANI and DDH results, strain CFH S0501T is considered to represent a novel species, for which the name Brevibacillus migulae sp.
32931405	10	5	theme	CFH	1197:1199	arg1	S0501T					1201:1206	CFH S0501T	1197:1206	CFH S0501T	1197:1206	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	2	6	attach	isolated	223:230	arg2	S0501T					89:94	Strain CFH S0501T	78:94	Strain CFH S0501T	78:94	Strain CFH S0501T, a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella, was isolated from a sediment sample collected from the Yellow River in Henan Province, PR China.
32931405	2	6	attach	isolated	223:230	arg2	micro-organism					176:189	a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism	97:189	a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella	97:216	Strain CFH S0501T, a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella, was isolated from a sediment sample collected from the Yellow River in Henan Province, PR China.
32931405	2	6	attach	isolated	223:230	arg1	sample					248:253	a sediment sample	237:253	a sediment sample collected from the Yellow River in Henan Province, PR China	237:313	Strain CFH S0501T, a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella, was isolated from a sediment sample collected from the Yellow River in Henan Province, PR China.
32931405	9	7	with	Mbp	1066:1068	arg1	content					1081:1087	a G+C content	1075:1087	a G+C content of 49.7 mol%	1075:1100	The genome size was 5.26 Mbp with a G+C content of 49.7 mol%.
32931405	2	8	theme	Yellow	274:279	arg1	China					309:313	PR China	306:313	PR China	306:313	Strain CFH S0501T, a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella, was isolated from a sediment sample collected from the Yellow River in Henan Province, PR China.
32931405	2	8	theme	Yellow	274:279	arg1	River					281:285	the Yellow River	270:285	the Yellow River in Henan Province	270:303	Strain CFH S0501T, a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella, was isolated from a sediment sample collected from the Yellow River in Henan Province, PR China.
32931405	4	9	theme	DSM	554:556	arg1	8445T					558:562	Brevibacillus centrosporus DSM 8445T	527:562	Brevibacillus centrosporus DSM 8445T	527:562	Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain belonged to the genus Brevibacillus and was closely related to Brevibacillus centrosporus DSM 8445T and Brevibacillus ginsengisoli Gsoil 3088T (with 96.8 and 96.7 % sequence similarity, respectively).
32931405	11	10	theme	Brevibacillus	1503:1515	arg1	sp					1525:1526	the name Brevibacillus migulae sp	1494:1526	the name Brevibacillus migulae sp	1494:1526	Based on physiological properties, chemotaxonomic characteristics and low ANI and DDH results, strain CFH S0501T is considered to represent a novel species, for which the name Brevibacillus migulae sp.
32931405	4	11	theme	gene	423:426	arg1	sequences					428:436	the 16S rRNA gene sequences	410:436	the 16S rRNA gene sequences	410:436	Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain belonged to the genus Brevibacillus and was closely related to Brevibacillus centrosporus DSM 8445T and Brevibacillus ginsengisoli Gsoil 3088T (with 96.8 and 96.7 % sequence similarity, respectively).
32931405	10	12	dep	low	1274:1276	arg1	ANIb					1294:1297	ANIb	1294:1297	ANIb	1294:1297	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	10	12	dep	low	1274:1276	arg1	%					1291:1291	ANIm <86.11 %	1279:1291	ANIm <86.11 %	1279:1291	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	10	12	dep	low	1274:1276	arg1	species					1222:1228	the other species	1212:1228	the other species of the genus Brevibacillus	1212:1255	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	10	12	dep	low	1274:1276	arg1	low					1274:1276	low	1274:1276	low	1274:1276	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	10	12	dep	low	1274:1276	arg1	values					1182:1187	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values	1103:1187	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T	1103:1206	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	1	13	theme	Yellow	48:53	arg1	sediment					61:68	a Yellow River sediment	46:68	a Yellow River sediment sample	46:75	nov., isolated from a Yellow River sediment sample.
32931405	6	14	dep	anteiso-C15 	735:746	arg1	iso-C15 					755:762	iso-C15 	755:762	iso-C15 	755:762	Major cellular fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
32931405	6	14	dep	anteiso-C15 	735:746	arg1	 0					748:749	 0	748:749	 0	748:749	Major cellular fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
32931405	6	14	dep	anteiso-C15 	735:746	arg1	 0					764:765	 0	764:765	 0	764:765	Major cellular fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
32931405	4	15	theme	centrosporus	541:552	arg1	8445T					558:562	Brevibacillus centrosporus DSM 8445T	527:562	Brevibacillus centrosporus DSM 8445T	527:562	Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain belonged to the genus Brevibacillus and was closely related to Brevibacillus centrosporus DSM 8445T and Brevibacillus ginsengisoli Gsoil 3088T (with 96.8 and 96.7 % sequence similarity, respectively).
32931405	3	16	theme	Optimum	316:322	arg1	growth					324:329	Optimum growth	316:329	Optimum growth	316:329	Optimum growth was observed at 28 °C, pH 7.0 and without NaCl.
32931405	2	17	theme	aerobic	126:132	arg1	micro-organism					176:189	a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism	97:189	a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella	97:216	Strain CFH S0501T, a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella, was isolated from a sediment sample collected from the Yellow River in Henan Province, PR China.
32931405	2	17	theme	aerobic	126:132	arg1	S0501T					89:94	Strain CFH S0501T	78:94	Strain CFH S0501T	78:94	Strain CFH S0501T, a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella, was isolated from a sediment sample collected from the Yellow River in Henan Province, PR China.
32931405	13	18	theme	29940T=BCRC	1583:1593	arg1	S0501T					1570:1575	CFH S0501T	1566:1575	CFH S0501T (=DSM 29940T=BCRC 80809T)	1566:1601	The type strain is CFH S0501T (=DSM 29940T=BCRC 80809T).
32931405	13	18	theme	29940T=BCRC	1583:1593	arg1	80809T					1595:1600	=DSM 29940T=BCRC 80809T	1578:1600	=DSM 29940T=BCRC 80809T	1578:1600	The type strain is CFH S0501T (=DSM 29940T=BCRC 80809T).
32931405	10	19	theme	genus	1237:1241	arg1	%					1291:1291	ANIm <86.11 %	1279:1291	ANIm <86.11 %	1279:1291	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	10	19	theme	genus	1237:1241	arg1	species					1222:1228	the other species	1212:1228	the other species of the genus Brevibacillus	1212:1255	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	10	19	theme	genus	1237:1241	arg1	low					1274:1276	low	1274:1276	low	1274:1276	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	10	19	theme	genus	1237:1241	arg1	values					1182:1187	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values	1103:1187	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T	1103:1206	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	1	20	theme	River	55:59	arg1	sediment					61:68	a Yellow River sediment	46:68	a Yellow River sediment sample	46:75	nov., isolated from a Yellow River sediment sample.
32931405	10	21	theme	 and	1307:1310	arg1	%					1323:1323	% and DDH <25.00 %	1306:1323	% and DDH <25.00 %	1306:1323	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	11	22	theme	physiological	1336:1348	arg1	properties					1350:1359	physiological properties	1336:1359	physiological properties	1336:1359	Based on physiological properties, chemotaxonomic characteristics and low ANI and DDH results, strain CFH S0501T is considered to represent a novel species, for which the name Brevibacillus migulae sp.
32931405	11	23	theme	name	1498:1501	arg1	sp					1525:1526	the name Brevibacillus migulae sp	1494:1526	the name Brevibacillus migulae sp	1494:1526	Based on physiological properties, chemotaxonomic characteristics and low ANI and DDH results, strain CFH S0501T is considered to represent a novel species, for which the name Brevibacillus migulae sp.
32931405	7	24	theme	unidentified	940:951	arg1	lipid					959:963	an unidentified polar lipid	937:963	an unidentified polar lipid	937:963	Polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, two unidentified phospholipids and an unidentified polar lipid.
32931405	1	25	theme	sediment	61:68	arg1	sample					70:75	a Yellow River sediment sample	46:75	a Yellow River sediment sample	46:75	nov., isolated from a Yellow River sediment sample.
32931405	11	26	theme	chemotaxonomic	1362:1375	arg1	characteristics					1377:1391	chemotaxonomic characteristics	1362:1391	chemotaxonomic characteristics	1362:1391	Based on physiological properties, chemotaxonomic characteristics and low ANI and DDH results, strain CFH S0501T is considered to represent a novel species, for which the name Brevibacillus migulae sp.
32931405	11	27	theme	low	1397:1399	arg1	ANI					1401:1403	low ANI and DDH results	1397:1419	ANI	1401:1403	Based on physiological properties, chemotaxonomic characteristics and low ANI and DDH results, strain CFH S0501T is considered to represent a novel species, for which the name Brevibacillus migulae sp.
32931405	0	28	theme	migulae	14:20	arg1	sp					22:23	Brevibacillus migulae sp	0:23	Brevibacillus migulae sp.	0:24	Brevibacillus migulae sp.
32931405	2	29	theme	Gram-stain-positive	105:123	arg1	micro-organism					176:189	a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism	97:189	a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella	97:216	Strain CFH S0501T, a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella, was isolated from a sediment sample collected from the Yellow River in Henan Province, PR China.
32931405	2	29	theme	Gram-stain-positive	105:123	arg1	S0501T					89:94	Strain CFH S0501T	78:94	Strain CFH S0501T	78:94	Strain CFH S0501T, a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella, was isolated from a sediment sample collected from the Yellow River in Henan Province, PR China.
32931405	7	30	theme	polar	953:957	arg1	lipid					959:963	an unidentified polar lipid	937:963	an unidentified polar lipid	937:963	Polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, two unidentified phospholipids and an unidentified polar lipid.
32931405	4	31	theme	rRNA	418:421	arg1	sequences					428:436	the 16S rRNA gene sequences	410:436	the 16S rRNA gene sequences	410:436	Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain belonged to the genus Brevibacillus and was closely related to Brevibacillus centrosporus DSM 8445T and Brevibacillus ginsengisoli Gsoil 3088T (with 96.8 and 96.7 % sequence similarity, respectively).
32931405	0	32	theme	Brevibacillus	0:12	arg1	sp					22:23	Brevibacillus migulae sp	0:23	Brevibacillus migulae sp.	0:24	Brevibacillus migulae sp.
32931405	4	33	theme	Phylogenetic	379:390	arg1	analysis					392:399	Phylogenetic analysis	379:399	Phylogenetic analysis based on the 16S rRNA gene sequences	379:436	Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain belonged to the genus Brevibacillus and was closely related to Brevibacillus centrosporus DSM 8445T and Brevibacillus ginsengisoli Gsoil 3088T (with 96.8 and 96.7 % sequence similarity, respectively).
32931405	10	34	theme	Brevibacillus	1243:1255	arg1	genus					1237:1241	the genus Brevibacillus	1233:1255	the genus Brevibacillus	1233:1255	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	9	35	theme	%	1100:1100	arg1	content					1081:1087	a G+C content	1075:1087	a G+C content of 49.7 mol%	1075:1100	The genome size was 5.26 Mbp with a G+C content of 49.7 mol%.
32931405	11	36	theme	CFH	1429:1431	arg1	S0501T					1433:1438	strain CFH S0501T	1422:1438	strain CFH S0501T	1422:1438	Based on physiological properties, chemotaxonomic characteristics and low ANI and DDH results, strain CFH S0501T is considered to represent a novel species, for which the name Brevibacillus migulae sp.
32931405	10	37	theme	in	1145:1146	arg1	DDH					1177:1179	DDH	1177:1179	DDH	1177:1179	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	10	37	theme	in	1145:1146	arg1	DNA-DNAhybridization					1155:1174	in silico DNA-DNAhybridization	1145:1174	in silico DNA-DNAhybridization (DDH)	1145:1180	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	11	38	theme	novel	1469:1473	arg1	species					1475:1481	a novel species	1467:1481	a novel species	1467:1481	Based on physiological properties, chemotaxonomic characteristics and low ANI and DDH results, strain CFH S0501T is considered to represent a novel species, for which the name Brevibacillus migulae sp.
32931405	10	39	theme	ANIm	1279:1282	arg1	ANIb					1294:1297	ANIb	1294:1297	ANIb	1294:1297	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	10	39	theme	ANIm	1279:1282	arg1	%					1291:1291	ANIm <86.11 %	1279:1291	ANIm <86.11 %	1279:1291	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	10	39	theme	ANIm	1279:1282	arg1	species					1222:1228	the other species	1212:1228	the other species of the genus Brevibacillus	1212:1255	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	10	39	theme	ANIm	1279:1282	arg1	low					1274:1276	low	1274:1276	low	1274:1276	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	10	39	theme	ANIm	1279:1282	arg1	values					1182:1187	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values	1103:1187	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T	1103:1206	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	10	40	theme	identity	1126:1133	arg1	%					1291:1291	ANIm <86.11 %	1279:1291	ANIm <86.11 %	1279:1291	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	10	40	theme	identity	1126:1133	arg1	low					1274:1276	low	1274:1276	low	1274:1276	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	10	40	theme	identity	1126:1133	arg1	values					1182:1187	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values	1103:1187	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T	1103:1206	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	4	41	dep	similarity	638:647	arg1	%					627:627	%	627:627	%	627:627	Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain belonged to the genus Brevibacillus and was closely related to Brevibacillus centrosporus DSM 8445T and Brevibacillus ginsengisoli Gsoil 3088T (with 96.8 and 96.7 % sequence similarity, respectively).
32931405	10	42	theme	average	1107:1113	arg1	ANI					1136:1138	ANI	1136:1138	ANI	1136:1138	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	10	42	theme	average	1107:1113	arg1	identity					1126:1133	average nucleotide identity	1107:1133	average nucleotide identity (ANI)	1107:1139	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	4	43	theme	Gsoil	595:599	arg1	3088T					601:605	Brevibacillus ginsengisoli Gsoil 3088T	568:605	Brevibacillus ginsengisoli Gsoil 3088T	568:605	Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain belonged to the genus Brevibacillus and was closely related to Brevibacillus centrosporus DSM 8445T and Brevibacillus ginsengisoli Gsoil 3088T (with 96.8 and 96.7 % sequence similarity, respectively).
32931405	13	44	theme	CFH	1566:1568	arg1	strain					1556:1561	The type strain	1547:1561	The type strain	1547:1561	The type strain is CFH S0501T (=DSM 29940T=BCRC 80809T).
32931405	13	44	theme	CFH	1566:1568	arg1	S0501T					1570:1575	CFH S0501T	1566:1575	CFH S0501T (=DSM 29940T=BCRC 80809T)	1566:1601	The type strain is CFH S0501T (=DSM 29940T=BCRC 80809T).
32931405	13	44	theme	CFH	1566:1568	arg1	80809T					1595:1600	=DSM 29940T=BCRC 80809T	1578:1600	=DSM 29940T=BCRC 80809T	1578:1600	The type strain is CFH S0501T (=DSM 29940T=BCRC 80809T).
32931405	10	45	theme	nucleotide	1115:1124	arg1	ANI					1136:1138	ANI	1136:1138	ANI	1136:1138	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	10	45	theme	nucleotide	1115:1124	arg1	identity					1126:1133	average nucleotide identity	1107:1133	average nucleotide identity (ANI)	1107:1139	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	2	46	theme	endospore-forming	147:163	arg1	micro-organism					176:189	a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism	97:189	a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella	97:216	Strain CFH S0501T, a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella, was isolated from a sediment sample collected from the Yellow River in Henan Province, PR China.
32931405	2	46	theme	endospore-forming	147:163	arg1	S0501T					89:94	Strain CFH S0501T	78:94	Strain CFH S0501T	78:94	Strain CFH S0501T, a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella, was isolated from a sediment sample collected from the Yellow River in Henan Province, PR China.
32931405	7	47	theme	Polar	768:772	arg1	lipids					774:779	Polar lipids	768:779	Polar lipids	768:779	Polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, two unidentified phospholipids and an unidentified polar lipid.
32931405	9	48	theme	49.7 mol	1092:1099	arg1	%					1100:1100	49.7 mol%	1092:1100	49.7 mol%	1092:1100	The genome size was 5.26 Mbp with a G+C content of 49.7 mol%.
32931405	2	49	theme	CFH	85:87	arg1	micro-organism					176:189	a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism	97:189	a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella	97:216	Strain CFH S0501T, a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella, was isolated from a sediment sample collected from the Yellow River in Henan Province, PR China.
32931405	2	49	theme	CFH	85:87	arg1	S0501T					89:94	Strain CFH S0501T	78:94	Strain CFH S0501T	78:94	Strain CFH S0501T, a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella, was isolated from a sediment sample collected from the Yellow River in Henan Province, PR China.
32931405	5	50	theme	predominant	669:679	arg1	MK-7					697:700	MK-7	697:700	MK-7	697:700	The predominant menaquinone was MK-7.
32931405	5	50	theme	predominant	669:679	arg1	menaquinone					681:691	The predominant menaquinone	665:691	The predominant menaquinone	665:691	The predominant menaquinone was MK-7.
32931405	4	51	theme	16S	414:416	arg1	sequences					428:436	the 16S rRNA gene sequences	410:436	the 16S rRNA gene sequences	410:436	Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain belonged to the genus Brevibacillus and was closely related to Brevibacillus centrosporus DSM 8445T and Brevibacillus ginsengisoli Gsoil 3088T (with 96.8 and 96.7 % sequence similarity, respectively).
32931405	8	52	contain	contain	1007:1013	arg1	peptidoglycan					980:992	The cell-wall peptidoglycan	966:992	The cell-wall peptidoglycan	966:992	The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid.
32931405	8	52	contain	contain	1007:1013	arg2	acid					1035:1038	meso-diaminopimelic acid	1015:1038	meso-diaminopimelic acid	1015:1038	The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid.
32931405	2	53	theme	Strain	78:83	arg1	micro-organism					176:189	a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism	97:189	a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella	97:216	Strain CFH S0501T, a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella, was isolated from a sediment sample collected from the Yellow River in Henan Province, PR China.
32931405	2	53	theme	Strain	78:83	arg1	S0501T					89:94	Strain CFH S0501T	78:94	Strain CFH S0501T	78:94	Strain CFH S0501T, a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella, was isolated from a sediment sample collected from the Yellow River in Henan Province, PR China.
32931405	11	54	theme	DDH	1409:1411	arg1	results					1413:1419	low ANI and DDH results	1397:1419	results	1413:1419	Based on physiological properties, chemotaxonomic characteristics and low ANI and DDH results, strain CFH S0501T is considered to represent a novel species, for which the name Brevibacillus migulae sp.
32931405	2	55	theme	rod-shaped	135:144	arg1	micro-organism					176:189	a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism	97:189	a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella	97:216	Strain CFH S0501T, a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella, was isolated from a sediment sample collected from the Yellow River in Henan Province, PR China.
32931405	2	55	theme	rod-shaped	135:144	arg1	S0501T					89:94	Strain CFH S0501T	78:94	Strain CFH S0501T	78:94	Strain CFH S0501T, a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella, was isolated from a sediment sample collected from the Yellow River in Henan Province, PR China.
32931405	6	56	theme	fatty	718:722	arg1	acids					724:728	Major cellular fatty acids	703:728	Major cellular fatty acids	703:728	Major cellular fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
32931405	13	57	theme	=DSM	1578:1581	arg1	S0501T					1570:1575	CFH S0501T	1566:1575	CFH S0501T (=DSM 29940T=BCRC 80809T)	1566:1601	The type strain is CFH S0501T (=DSM 29940T=BCRC 80809T).
32931405	13	57	theme	=DSM	1578:1581	arg1	80809T					1595:1600	=DSM 29940T=BCRC 80809T	1578:1600	=DSM 29940T=BCRC 80809T	1578:1600	The type strain is CFH S0501T (=DSM 29940T=BCRC 80809T).
32931405	10	58	dep	in	1145:1146	arg1	silico					1148:1153	silico	1148:1153	silico	1148:1153	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	4	59	theme	Brevibacillus	486:498	arg1	genus					480:484	the genus Brevibacillus	476:498	the genus Brevibacillus	476:498	Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain belonged to the genus Brevibacillus and was closely related to Brevibacillus centrosporus DSM 8445T and Brevibacillus ginsengisoli Gsoil 3088T (with 96.8 and 96.7 % sequence similarity, respectively).
32931405	2	60	theme	novel	99:103	arg1	micro-organism					176:189	a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism	97:189	a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella	97:216	Strain CFH S0501T, a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella, was isolated from a sediment sample collected from the Yellow River in Henan Province, PR China.
32931405	2	60	theme	novel	99:103	arg1	S0501T					89:94	Strain CFH S0501T	78:94	Strain CFH S0501T	78:94	Strain CFH S0501T, a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella, was isolated from a sediment sample collected from the Yellow River in Henan Province, PR China.
32931405	2	61	theme	peritrichous	196:207	arg1	flagella					209:216	peritrichous flagella	196:216	peritrichous flagella	196:216	Strain CFH S0501T, a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella, was isolated from a sediment sample collected from the Yellow River in Henan Province, PR China.
32931405	4	62	with	8445T	558:562	arg1	similarity					638:647	96.8 and 96.7 % sequence similarity	613:647	96.8 and 96.7 % sequence similarity	613:647	Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain belonged to the genus Brevibacillus and was closely related to Brevibacillus centrosporus DSM 8445T and Brevibacillus ginsengisoli Gsoil 3088T (with 96.8 and 96.7 % sequence similarity, respectively).
32931405	6	63	theme	cellular	709:716	arg1	acids					724:728	Major cellular fatty acids	703:728	Major cellular fatty acids	703:728	Major cellular fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
32931405	7	64	theme	unidentified	906:917	arg1	phospholipids					919:931	two unidentified phospholipids	902:931	two unidentified phospholipids	902:931	Polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, two unidentified phospholipids and an unidentified polar lipid.
32931405	8	65	theme	meso-diaminopimelic	1015:1033	arg1	acid					1035:1038	meso-diaminopimelic acid	1015:1038	meso-diaminopimelic acid	1015:1038	The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid.
32931405	4	66	dep	%	627:627	arg1	96.7 					622:626	96.7 	622:626	96.7 	622:626	Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain belonged to the genus Brevibacillus and was closely related to Brevibacillus centrosporus DSM 8445T and Brevibacillus ginsengisoli Gsoil 3088T (with 96.8 and 96.7 % sequence similarity, respectively).
32931405	4	66	dep	%	627:627	arg1	96.8					613:616	96.8	613:616	96.8	613:616	Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain belonged to the genus Brevibacillus and was closely related to Brevibacillus centrosporus DSM 8445T and Brevibacillus ginsengisoli Gsoil 3088T (with 96.8 and 96.7 % sequence similarity, respectively).
32931405	6	67	theme	Major	703:707	arg1	acids					724:728	Major cellular fatty acids	703:728	Major cellular fatty acids	703:728	Major cellular fatty acids were anteiso-C15 : 0 and iso-C15 : 0.
32931405	2	68	theme	PR	306:307	arg1	China					309:313	PR China	306:313	PR China	306:313	Strain CFH S0501T, a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella, was isolated from a sediment sample collected from the Yellow River in Henan Province, PR China.
32931405	2	68	theme	PR	306:307	arg1	River					281:285	the Yellow River	270:285	the Yellow River in Henan Province	270:303	Strain CFH S0501T, a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella, was isolated from a sediment sample collected from the Yellow River in Henan Province, PR China.
32931405	9	69	theme	genome	1045:1050	arg1	size					1052:1055	The genome size	1041:1055	The genome size	1041:1055	The genome size was 5.26 Mbp with a G+C content of 49.7 mol%.
32931405	9	69	theme	genome	1045:1050	arg1	Mbp					1066:1068	5.26 Mbp	1061:1068	5.26 Mbp	1061:1068	The genome size was 5.26 Mbp with a G+C content of 49.7 mol%.
32931405	11	70	theme	migulae	1517:1523	arg1	sp					1525:1526	the name Brevibacillus migulae sp	1494:1526	the name Brevibacillus migulae sp	1494:1526	Based on physiological properties, chemotaxonomic characteristics and low ANI and DDH results, strain CFH S0501T is considered to represent a novel species, for which the name Brevibacillus migulae sp.
32931405	4	71	with	3088T	601:605	arg1	similarity					638:647	96.8 and 96.7 % sequence similarity	613:647	96.8 and 96.7 % sequence similarity	613:647	Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain belonged to the genus Brevibacillus and was closely related to Brevibacillus centrosporus DSM 8445T and Brevibacillus ginsengisoli Gsoil 3088T (with 96.8 and 96.7 % sequence similarity, respectively).
32931405	2	72	theme	sediment	239:246	arg1	sample					248:253	a sediment sample	237:253	a sediment sample collected from the Yellow River in Henan Province, PR China	237:313	Strain CFH S0501T, a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella, was isolated from a sediment sample collected from the Yellow River in Henan Province, PR China.
32931405	10	73	dep	%	1291:1291	arg1	%					1323:1323	% and DDH <25.00 %	1306:1323	% and DDH <25.00 %	1306:1323	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	2	74	theme	motile	169:174	arg1	micro-organism					176:189	a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism	97:189	a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella	97:216	Strain CFH S0501T, a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella, was isolated from a sediment sample collected from the Yellow River in Henan Province, PR China.
32931405	2	74	theme	motile	169:174	arg1	S0501T					89:94	Strain CFH S0501T	78:94	Strain CFH S0501T	78:94	Strain CFH S0501T, a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella, was isolated from a sediment sample collected from the Yellow River in Henan Province, PR China.
32931405	10	75	theme	DDH	1312:1314	arg1	%					1323:1323	% and DDH <25.00 %	1306:1323	% and DDH <25.00 %	1306:1323	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	2	76	with	micro-organism	176:189	arg1	flagella					209:216	peritrichous flagella	196:216	peritrichous flagella	196:216	Strain CFH S0501T, a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella, was isolated from a sediment sample collected from the Yellow River in Henan Province, PR China.
32931405	10	77	theme	other	1216:1220	arg1	%					1291:1291	ANIm <86.11 %	1279:1291	ANIm <86.11 %	1279:1291	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	10	77	theme	other	1216:1220	arg1	species					1222:1228	the other species	1212:1228	the other species of the genus Brevibacillus	1212:1255	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	10	77	theme	other	1216:1220	arg1	low					1274:1276	low	1274:1276	low	1274:1276	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	4	78	theme	ginsengisoli	582:593	arg1	3088T					601:605	Brevibacillus ginsengisoli Gsoil 3088T	568:605	Brevibacillus ginsengisoli Gsoil 3088T	568:605	Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain belonged to the genus Brevibacillus and was closely related to Brevibacillus centrosporus DSM 8445T and Brevibacillus ginsengisoli Gsoil 3088T (with 96.8 and 96.7 % sequence similarity, respectively).
32931405	3	79	located	observed	335:342	arg1	°C					350:351	28 °C	347:351	28 °C	347:351	Optimum growth was observed at 28 °C, pH 7.0 and without NaCl.
32931405	3	79	located	observed	335:342	arg2	growth					324:329	Optimum growth	316:329	Optimum growth	316:329	Optimum growth was observed at 28 °C, pH 7.0 and without NaCl.
32931405	3	79	located	observed	335:342	arg1	pH					354:355	pH 7.0	354:359	pH 7.0	354:359	Optimum growth was observed at 28 °C, pH 7.0 and without NaCl.
32931405	10	80	theme	DNA-DNAhybridization	1155:1174	arg1	%					1291:1291	ANIm <86.11 %	1279:1291	ANIm <86.11 %	1279:1291	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	10	80	theme	DNA-DNAhybridization	1155:1174	arg1	low					1274:1276	low	1274:1276	low	1274:1276	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	10	80	theme	DNA-DNAhybridization	1155:1174	arg1	values					1182:1187	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values	1103:1187	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T	1103:1206	The average nucleotide identity (ANI) and in silico DNA-DNAhybridization (DDH) values between CFH S0501T and the other species of the genus Brevibacillus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %).
32931405	8	81	theme	cell-wall	970:978	arg1	peptidoglycan					980:992	The cell-wall peptidoglycan	966:992	The cell-wall peptidoglycan	966:992	The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid.
32931405	4	82	theme	 sequence	628:636	arg1	similarity					638:647	96.8 and 96.7 % sequence similarity	613:647	96.8 and 96.7 % sequence similarity	613:647	Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain belonged to the genus Brevibacillus and was closely related to Brevibacillus centrosporus DSM 8445T and Brevibacillus ginsengisoli Gsoil 3088T (with 96.8 and 96.7 % sequence similarity, respectively).
32931405	9	83	theme	G+C	1077:1079	arg1	content					1081:1087	a G+C content	1075:1087	a G+C content of 49.7 mol%	1075:1100	The genome size was 5.26 Mbp with a G+C content of 49.7 mol%.
32931405	4	84	theme	Brevibacillus	568:580	arg1	3088T					601:605	Brevibacillus ginsengisoli Gsoil 3088T	568:605	Brevibacillus ginsengisoli Gsoil 3088T	568:605	Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain belonged to the genus Brevibacillus and was closely related to Brevibacillus centrosporus DSM 8445T and Brevibacillus ginsengisoli Gsoil 3088T (with 96.8 and 96.7 % sequence similarity, respectively).
32459165	11	0	theme	31163T=DSM	1639:1648	arg1	LMM-1652T					1623:1631	the name Corynebacterium urogenitale LMM-1652T	1586:1631	the name Corynebacterium urogenitale LMM-1652T (=LMG 31163T=DSM 108747T)	1586:1657	We propose a novel species of the genus Corynebacterium with the name Corynebacterium urogenitale LMM-1652T (=LMG 31163T=DSM 108747T).
32459165	11	0	theme	31163T=DSM	1639:1648	arg1	108747T					1650:1656	=LMG 31163T=DSM 108747T	1634:1656	=LMG 31163T=DSM 108747T	1634:1656	We propose a novel species of the genus Corynebacterium with the name Corynebacterium urogenitale LMM-1652T (=LMG 31163T=DSM 108747T).
32459165	5	1	theme	%	694:694	arg1	range					675:679	the range	671:679	the range of 23.2-24.8 %	671:694	Subsequent whole genome analysis showed that the genome-to-genome distance of strain LMM-1652T to its closest relatives was in the range of 23.2-24.8 %, while the average nucleotide identity values ranged from 73.7 to 74.3%, thus confirming that this isolate represents a novel species.
32459165	9	2	theme	peptidoglycan	1430:1442	arg1	acid					1418:1421	the diagnostic diamino acid	1395:1421	the diagnostic diamino acid of the peptidoglycan	1395:1442	The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
32459165	9	2	theme	peptidoglycan	1430:1442	arg1	acid					1387:1390	meso-diaminopimelic acid	1367:1390	meso-diaminopimelic acid	1367:1390	The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
32459165	11	3	theme	Corynebacterium	1595:1609	arg1	LMM-1652T					1623:1631	the name Corynebacterium urogenitale LMM-1652T	1586:1631	the name Corynebacterium urogenitale LMM-1652T (=LMG 31163T=DSM 108747T)	1586:1657	We propose a novel species of the genus Corynebacterium with the name Corynebacterium urogenitale LMM-1652T (=LMG 31163T=DSM 108747T).
32459165	11	3	theme	Corynebacterium	1595:1609	arg1	108747T					1650:1656	=LMG 31163T=DSM 108747T	1634:1656	=LMG 31163T=DSM 108747T	1634:1656	We propose a novel species of the genus Corynebacterium with the name Corynebacterium urogenitale LMM-1652T (=LMG 31163T=DSM 108747T).
32459165	7	4	theme	unidentified	1108:1119	arg1	lipid					1121:1125	one unidentified lipid	1104:1125	one unidentified lipid lacking any functional group	1104:1154	The polar lipids profile of the strain consisted mainly of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol-mannoside, as well as one unidentified lipid lacking any functional group.
32459165	5	5	theme	genome-to-genome	593:608	arg1	distance					610:617	the genome-to-genome distance	589:617	the genome-to-genome distance of strain LMM-1652T to its closest relatives	589:662	Subsequent whole genome analysis showed that the genome-to-genome distance of strain LMM-1652T to its closest relatives was in the range of 23.2-24.8 %, while the average nucleotide identity values ranged from 73.7 to 74.3%, thus confirming that this isolate represents a novel species.
32459165	10	6	theme	acid	1455:1458	arg1	profile					1460:1466	The fatty acid profile	1445:1466	The fatty acid profile	1445:1466	The fatty acid profile was mainly composed of C18 : 1 ω9c, C18 : 0 and C16 : 0.
32459165	5	7	theme	nucleotide	715:724	arg1	values					735:740	the average nucleotide identity values	703:740	the average nucleotide identity values	703:740	Subsequent whole genome analysis showed that the genome-to-genome distance of strain LMM-1652T to its closest relatives was in the range of 23.2-24.8 %, while the average nucleotide identity values ranged from 73.7 to 74.3%, thus confirming that this isolate represents a novel species.
32459165	2	8	theme	bacterial	101:109	arg1	isolate					111:117	A Gram-stain-positive bacterial isolate	79:117	A Gram-stain-positive bacterial isolate	79:117	A Gram-stain-positive bacterial isolate, designated LMM-1652T, was isolated from an intrauterine cytobrush sample originating from a postpartum Holstein Friesian dairy cow.
32459165	4	9	theme	closest	396:402	arg1	relatives					404:412	its closest relatives	392:412	its closest relatives	392:412	16S rRNA gene sequence similarity analyses revealed that its closest relatives were Corynebacterium falsenii (97.05 % similarity), Corynebacterium jeikeium (96.83 %) and Corynebacterium urealyticum (96.82 %).
32459165	1	10	theme	genital	55:61	arg1	tract					63:67	the genital tract	51:67	the genital tract of a cow	51:76	nov. isolated from the genital tract of a cow.
32459165	11	11	theme	name	1590:1593	arg1	LMM-1652T					1623:1631	the name Corynebacterium urogenitale LMM-1652T	1586:1631	the name Corynebacterium urogenitale LMM-1652T (=LMG 31163T=DSM 108747T)	1586:1657	We propose a novel species of the genus Corynebacterium with the name Corynebacterium urogenitale LMM-1652T (=LMG 31163T=DSM 108747T).
32459165	11	11	theme	name	1590:1593	arg1	108747T					1650:1656	=LMG 31163T=DSM 108747T	1634:1656	=LMG 31163T=DSM 108747T	1634:1656	We propose a novel species of the genus Corynebacterium with the name Corynebacterium urogenitale LMM-1652T (=LMG 31163T=DSM 108747T).
32459165	5	12	theme	identity	726:733	arg1	values					735:740	the average nucleotide identity values	703:740	the average nucleotide identity values	703:740	Subsequent whole genome analysis showed that the genome-to-genome distance of strain LMM-1652T to its closest relatives was in the range of 23.2-24.8 %, while the average nucleotide identity values ranged from 73.7 to 74.3%, thus confirming that this isolate represents a novel species.
32459165	5	13	theme	Subsequent	544:553	arg1	analysis					568:575	Subsequent whole genome analysis	544:575	Subsequent whole genome analysis	544:575	Subsequent whole genome analysis showed that the genome-to-genome distance of strain LMM-1652T to its closest relatives was in the range of 23.2-24.8 %, while the average nucleotide identity values ranged from 73.7 to 74.3%, thus confirming that this isolate represents a novel species.
32459165	11	14	theme	urogenitale	1611:1621	arg1	LMM-1652T					1623:1631	the name Corynebacterium urogenitale LMM-1652T	1586:1631	the name Corynebacterium urogenitale LMM-1652T (=LMG 31163T=DSM 108747T)	1586:1657	We propose a novel species of the genus Corynebacterium with the name Corynebacterium urogenitale LMM-1652T (=LMG 31163T=DSM 108747T).
32459165	11	14	theme	urogenitale	1611:1621	arg1	108747T					1650:1656	=LMG 31163T=DSM 108747T	1634:1656	=LMG 31163T=DSM 108747T	1634:1656	We propose a novel species of the genus Corynebacterium with the name Corynebacterium urogenitale LMM-1652T (=LMG 31163T=DSM 108747T).
32459165	8	15	theme	unidentified	1279:1290	arg1	lipids					1292:1297	four unidentified lipids	1274:1297	four unidentified lipids	1274:1297	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	6	16	theme	quinone	871:877	arg1	system					879:884	a quinone system	869:884	a quinone system mainly consisting of MK-9(H2) and MK-10(H2)	869:928	Strain LMM-1652T was characterized by a quinone system mainly consisting of MK-9(H2) and MK-10(H2).
32459165	5	17	theme	LMM-1652T	629:637	arg1	distance					610:617	the genome-to-genome distance	589:617	the genome-to-genome distance of strain LMM-1652T to its closest relatives	589:662	Subsequent whole genome analysis showed that the genome-to-genome distance of strain LMM-1652T to its closest relatives was in the range of 23.2-24.8 %, while the average nucleotide identity values ranged from 73.7 to 74.3%, thus confirming that this isolate represents a novel species.
32459165	5	18	theme	strain	622:627	arg1	LMM-1652T					629:637	strain LMM-1652T	622:637	strain LMM-1652T	622:637	Subsequent whole genome analysis showed that the genome-to-genome distance of strain LMM-1652T to its closest relatives was in the range of 23.2-24.8 %, while the average nucleotide identity values ranged from 73.7 to 74.3%, thus confirming that this isolate represents a novel species.
32459165	8	19	theme	β-gentiobiosyl	1240:1253	arg1	diacylglycerol					1255:1268	β-gentiobiosyl diacylglycerol	1240:1268	β-gentiobiosyl diacylglycerol	1240:1268	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	8	20	theme	Smaller	1157:1163	arg1	diacylglycerol					1255:1268	β-gentiobiosyl diacylglycerol	1240:1268	β-gentiobiosyl diacylglycerol	1240:1268	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	8	20	theme	Smaller	1157:1163	arg1	phospholipids					1194:1206	four unidentified phospholipids	1176:1206	four unidentified phospholipids	1176:1206	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	8	20	theme	Smaller	1157:1163	arg1	lipids					1292:1297	four unidentified lipids	1274:1297	four unidentified lipids	1274:1297	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	8	20	theme	Smaller	1157:1163	arg1	glycolipids					1227:1237	four unidentified glycolipids	1209:1237	four unidentified glycolipids	1209:1237	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	8	20	theme	Smaller	1157:1163	arg1	amounts					1165:1171	Smaller amounts	1157:1171	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group	1157:1324	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	10	21	theme	fatty	1449:1453	arg1	profile					1460:1466	The fatty acid profile	1445:1466	The fatty acid profile	1445:1466	The fatty acid profile was mainly composed of C18 : 1 ω9c, C18 : 0 and C16 : 0.
32459165	5	22	dep	%	766:766	arg1	to					759:760	to	759:760	to	759:760	Subsequent whole genome analysis showed that the genome-to-genome distance of strain LMM-1652T to its closest relatives was in the range of 23.2-24.8 %, while the average nucleotide identity values ranged from 73.7 to 74.3%, thus confirming that this isolate represents a novel species.
32459165	1	23	theme	cow	74:76	arg1	tract					63:67	the genital tract	51:67	the genital tract of a cow	51:76	nov. isolated from the genital tract of a cow.
32459165	11	24	theme	genus	1559:1563	arg1	Corynebacterium					1565:1579	the genus Corynebacterium	1555:1579	the genus Corynebacterium	1555:1579	We propose a novel species of the genus Corynebacterium with the name Corynebacterium urogenitale LMM-1652T (=LMG 31163T=DSM 108747T).
32459165	9	25	theme	cell	1347:1350	arg1	wall					1352:1355	The cell wall	1343:1355	The cell wall	1343:1355	The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
32459165	5	26	theme	closest	646:652	arg1	relatives					654:662	its closest relatives	642:662	its closest relatives	642:662	Subsequent whole genome analysis showed that the genome-to-genome distance of strain LMM-1652T to its closest relatives was in the range of 23.2-24.8 %, while the average nucleotide identity values ranged from 73.7 to 74.3%, thus confirming that this isolate represents a novel species.
32459165	8	27	theme	diacylglycerol	1255:1268	arg1	diacylglycerol					1255:1268	β-gentiobiosyl diacylglycerol	1240:1268	β-gentiobiosyl diacylglycerol	1240:1268	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	8	27	theme	diacylglycerol	1255:1268	arg1	phospholipids					1194:1206	four unidentified phospholipids	1176:1206	four unidentified phospholipids	1176:1206	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	8	27	theme	diacylglycerol	1255:1268	arg1	lipids					1292:1297	four unidentified lipids	1274:1297	four unidentified lipids	1274:1297	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	8	27	theme	diacylglycerol	1255:1268	arg1	glycolipids					1227:1237	four unidentified glycolipids	1209:1237	four unidentified glycolipids	1209:1237	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	8	27	theme	diacylglycerol	1255:1268	arg1	amounts					1165:1171	Smaller amounts	1157:1171	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group	1157:1324	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	8	28	theme	phospholipids	1194:1206	arg1	diacylglycerol					1255:1268	β-gentiobiosyl diacylglycerol	1240:1268	β-gentiobiosyl diacylglycerol	1240:1268	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	8	28	theme	phospholipids	1194:1206	arg1	phospholipids					1194:1206	four unidentified phospholipids	1176:1206	four unidentified phospholipids	1176:1206	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	8	28	theme	phospholipids	1194:1206	arg1	lipids					1292:1297	four unidentified lipids	1274:1297	four unidentified lipids	1274:1297	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	8	28	theme	phospholipids	1194:1206	arg1	glycolipids					1227:1237	four unidentified glycolipids	1209:1237	four unidentified glycolipids	1209:1237	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	8	28	theme	phospholipids	1194:1206	arg1	amounts					1165:1171	Smaller amounts	1157:1171	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group	1157:1324	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	11	29	theme	novel	1538:1542	arg1	species					1544:1550	a novel species	1536:1550	a novel species of the genus Corynebacterium with the name Corynebacterium urogenitale LMM-1652T (=LMG 31163T=DSM 108747T)	1536:1657	We propose a novel species of the genus Corynebacterium with the name Corynebacterium urogenitale LMM-1652T (=LMG 31163T=DSM 108747T).
32459165	3	30	contain	had	263:265	arg2	rod					269:271	a rod	267:271	a rod	267:271	The strain had a rod to coccoid-shape, was catalase-positive and oxidase-negative.
32459165	3	30	contain	had	263:265	arg1	strain					256:261	The strain	252:261	The strain	252:261	The strain had a rod to coccoid-shape, was catalase-positive and oxidase-negative.
32459165	7	31	theme	strain	963:968	arg1	profile					948:954	The polar lipids profile	931:954	The polar lipids profile of the strain	931:968	The polar lipids profile of the strain consisted mainly of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol-mannoside, as well as one unidentified lipid lacking any functional group.
32459165	8	32	theme	unidentified	1181:1192	arg1	phospholipids					1194:1206	four unidentified phospholipids	1176:1206	four unidentified phospholipids	1176:1206	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	11	33	with	species	1544:1550	arg1	LMM-1652T					1623:1631	the name Corynebacterium urogenitale LMM-1652T	1586:1631	the name Corynebacterium urogenitale LMM-1652T (=LMG 31163T=DSM 108747T)	1586:1657	We propose a novel species of the genus Corynebacterium with the name Corynebacterium urogenitale LMM-1652T (=LMG 31163T=DSM 108747T).
32459165	11	33	with	species	1544:1550	arg1	108747T					1650:1656	=LMG 31163T=DSM 108747T	1634:1656	=LMG 31163T=DSM 108747T	1634:1656	We propose a novel species of the genus Corynebacterium with the name Corynebacterium urogenitale LMM-1652T (=LMG 31163T=DSM 108747T).
32459165	5	34	theme	whole	555:559	arg1	analysis					568:575	Subsequent whole genome analysis	544:575	Subsequent whole genome analysis	544:575	Subsequent whole genome analysis showed that the genome-to-genome distance of strain LMM-1652T to its closest relatives was in the range of 23.2-24.8 %, while the average nucleotide identity values ranged from 73.7 to 74.3%, thus confirming that this isolate represents a novel species.
32459165	2	35	theme	cytobrush	176:184	arg1	sample					186:191	an intrauterine cytobrush sample	160:191	an intrauterine cytobrush sample originating from a postpartum Holstein Friesian dairy cow	160:249	A Gram-stain-positive bacterial isolate, designated LMM-1652T, was isolated from an intrauterine cytobrush sample originating from a postpartum Holstein Friesian dairy cow.
32459165	5	36	theme	novel	816:820	arg1	species					822:828	a novel species	814:828	a novel species	814:828	Subsequent whole genome analysis showed that the genome-to-genome distance of strain LMM-1652T to its closest relatives was in the range of 23.2-24.8 %, while the average nucleotide identity values ranged from 73.7 to 74.3%, thus confirming that this isolate represents a novel species.
32459165	9	37	theme	meso-diaminopimelic	1367:1385	arg1	acid					1418:1421	the diagnostic diamino acid	1395:1421	the diagnostic diamino acid of the peptidoglycan	1395:1442	The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
32459165	9	37	theme	meso-diaminopimelic	1367:1385	arg1	acid					1387:1390	meso-diaminopimelic acid	1367:1390	meso-diaminopimelic acid	1367:1390	The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
32459165	2	38	theme	intrauterine	163:174	arg1	sample					186:191	an intrauterine cytobrush sample	160:191	an intrauterine cytobrush sample originating from a postpartum Holstein Friesian dairy cow	160:249	A Gram-stain-positive bacterial isolate, designated LMM-1652T, was isolated from an intrauterine cytobrush sample originating from a postpartum Holstein Friesian dairy cow.
32459165	2	39	theme	dairy	241:245	arg1	cow					247:249	a postpartum Holstein Friesian dairy cow	210:249	a postpartum Holstein Friesian dairy cow	210:249	A Gram-stain-positive bacterial isolate, designated LMM-1652T, was isolated from an intrauterine cytobrush sample originating from a postpartum Holstein Friesian dairy cow.
32459165	7	40	theme	functional	1139:1148	arg1	group					1150:1154	any functional group	1135:1154	any functional group	1135:1154	The polar lipids profile of the strain consisted mainly of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol-mannoside, as well as one unidentified lipid lacking any functional group.
32459165	2	41	theme	Gram-stain-positive	81:99	arg1	isolate					111:117	A Gram-stain-positive bacterial isolate	79:117	A Gram-stain-positive bacterial isolate	79:117	A Gram-stain-positive bacterial isolate, designated LMM-1652T, was isolated from an intrauterine cytobrush sample originating from a postpartum Holstein Friesian dairy cow.
32459165	5	42	theme	average	707:713	arg1	values					735:740	the average nucleotide identity values	703:740	the average nucleotide identity values	703:740	Subsequent whole genome analysis showed that the genome-to-genome distance of strain LMM-1652T to its closest relatives was in the range of 23.2-24.8 %, while the average nucleotide identity values ranged from 73.7 to 74.3%, thus confirming that this isolate represents a novel species.
32459165	2	43	theme	Friesian	232:239	arg1	cow					247:249	a postpartum Holstein Friesian dairy cow	210:249	a postpartum Holstein Friesian dairy cow	210:249	A Gram-stain-positive bacterial isolate, designated LMM-1652T, was isolated from an intrauterine cytobrush sample originating from a postpartum Holstein Friesian dairy cow.
32459165	4	44	theme	gene	344:347	arg1	similarity					358:367	16S rRNA gene sequence similarity	335:367	16S rRNA gene sequence similarity analyses	335:376	16S rRNA gene sequence similarity analyses revealed that its closest relatives were Corynebacterium falsenii (97.05 % similarity), Corynebacterium jeikeium (96.83 %) and Corynebacterium urealyticum (96.82 %).
32459165	8	45	theme	lipids	1292:1297	arg1	diacylglycerol					1255:1268	β-gentiobiosyl diacylglycerol	1240:1268	β-gentiobiosyl diacylglycerol	1240:1268	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	8	45	theme	lipids	1292:1297	arg1	phospholipids					1194:1206	four unidentified phospholipids	1176:1206	four unidentified phospholipids	1176:1206	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	8	45	theme	lipids	1292:1297	arg1	lipids					1292:1297	four unidentified lipids	1274:1297	four unidentified lipids	1274:1297	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	8	45	theme	lipids	1292:1297	arg1	glycolipids					1227:1237	four unidentified glycolipids	1209:1237	four unidentified glycolipids	1209:1237	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	8	45	theme	lipids	1292:1297	arg1	amounts					1165:1171	Smaller amounts	1157:1171	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group	1157:1324	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	8	46	theme	glycolipids	1227:1237	arg1	diacylglycerol					1255:1268	β-gentiobiosyl diacylglycerol	1240:1268	β-gentiobiosyl diacylglycerol	1240:1268	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	8	46	theme	glycolipids	1227:1237	arg1	phospholipids					1194:1206	four unidentified phospholipids	1176:1206	four unidentified phospholipids	1176:1206	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	8	46	theme	glycolipids	1227:1237	arg1	lipids					1292:1297	four unidentified lipids	1274:1297	four unidentified lipids	1274:1297	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	8	46	theme	glycolipids	1227:1237	arg1	glycolipids					1227:1237	four unidentified glycolipids	1209:1237	four unidentified glycolipids	1209:1237	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	8	46	theme	glycolipids	1227:1237	arg1	amounts					1165:1171	Smaller amounts	1157:1171	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group	1157:1324	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	6	47	theme	Strain	831:836	arg1	LMM-1652T					838:846	Strain LMM-1652T	831:846	Strain LMM-1652T	831:846	Strain LMM-1652T was characterized by a quinone system mainly consisting of MK-9(H2) and MK-10(H2).
32459165	2	48	theme	Holstein	223:230	arg1	cow					247:249	a postpartum Holstein Friesian dairy cow	210:249	a postpartum Holstein Friesian dairy cow	210:249	A Gram-stain-positive bacterial isolate, designated LMM-1652T, was isolated from an intrauterine cytobrush sample originating from a postpartum Holstein Friesian dairy cow.
32459165	4	49	theme	rRNA	339:342	arg1	similarity					358:367	16S rRNA gene sequence similarity	335:367	16S rRNA gene sequence similarity analyses	335:376	16S rRNA gene sequence similarity analyses revealed that its closest relatives were Corynebacterium falsenii (97.05 % similarity), Corynebacterium jeikeium (96.83 %) and Corynebacterium urealyticum (96.82 %).
32459165	8	50	theme	functional	1309:1318	arg1	group					1320:1324	a functional group	1307:1324	a functional group	1307:1324	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	8	51	theme	unidentified	1214:1225	arg1	glycolipids					1227:1237	four unidentified glycolipids	1209:1237	four unidentified glycolipids	1209:1237	Smaller amounts of four unidentified phospholipids, four unidentified glycolipids, β-gentiobiosyl diacylglycerol and four unidentified lipids lacking a functional group were also found.
32459165	11	52	theme	=LMG	1634:1637	arg1	LMM-1652T					1623:1631	the name Corynebacterium urogenitale LMM-1652T	1586:1631	the name Corynebacterium urogenitale LMM-1652T (=LMG 31163T=DSM 108747T)	1586:1657	We propose a novel species of the genus Corynebacterium with the name Corynebacterium urogenitale LMM-1652T (=LMG 31163T=DSM 108747T).
32459165	11	52	theme	=LMG	1634:1637	arg1	108747T					1650:1656	=LMG 31163T=DSM 108747T	1634:1656	=LMG 31163T=DSM 108747T	1634:1656	We propose a novel species of the genus Corynebacterium with the name Corynebacterium urogenitale LMM-1652T (=LMG 31163T=DSM 108747T).
32459165	2	53	theme	postpartum	212:221	arg1	cow					247:249	a postpartum Holstein Friesian dairy cow	210:249	a postpartum Holstein Friesian dairy cow	210:249	A Gram-stain-positive bacterial isolate, designated LMM-1652T, was isolated from an intrauterine cytobrush sample originating from a postpartum Holstein Friesian dairy cow.
32459165	4	54	theme	similarity	358:367	arg1	analyses					369:376	16S rRNA gene sequence similarity analyses	335:376	16S rRNA gene sequence similarity analyses	335:376	16S rRNA gene sequence similarity analyses revealed that its closest relatives were Corynebacterium falsenii (97.05 % similarity), Corynebacterium jeikeium (96.83 %) and Corynebacterium urealyticum (96.82 %).
32459165	2	55	attach	isolated	146:153	arg1	sample					186:191	an intrauterine cytobrush sample	160:191	an intrauterine cytobrush sample originating from a postpartum Holstein Friesian dairy cow	160:249	A Gram-stain-positive bacterial isolate, designated LMM-1652T, was isolated from an intrauterine cytobrush sample originating from a postpartum Holstein Friesian dairy cow.
32459165	2	55	attach	isolated	146:153	arg2	isolate					111:117	A Gram-stain-positive bacterial isolate	79:117	A Gram-stain-positive bacterial isolate	79:117	A Gram-stain-positive bacterial isolate, designated LMM-1652T, was isolated from an intrauterine cytobrush sample originating from a postpartum Holstein Friesian dairy cow.
32459165	9	56	theme	diagnostic	1399:1408	arg1	acid					1418:1421	the diagnostic diamino acid	1395:1421	the diagnostic diamino acid of the peptidoglycan	1395:1442	The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
32459165	9	56	theme	diagnostic	1399:1408	arg1	acid					1387:1390	meso-diaminopimelic acid	1367:1390	meso-diaminopimelic acid	1367:1390	The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
32459165	4	57	theme	sequence	349:356	arg1	similarity					358:367	16S rRNA gene sequence similarity	335:367	16S rRNA gene sequence similarity analyses	335:376	16S rRNA gene sequence similarity analyses revealed that its closest relatives were Corynebacterium falsenii (97.05 % similarity), Corynebacterium jeikeium (96.83 %) and Corynebacterium urealyticum (96.82 %).
32459165	9	58	theme	diamino	1410:1416	arg1	acid					1418:1421	the diagnostic diamino acid	1395:1421	the diagnostic diamino acid of the peptidoglycan	1395:1442	The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
32459165	9	58	theme	diamino	1410:1416	arg1	acid					1387:1390	meso-diaminopimelic acid	1367:1390	meso-diaminopimelic acid	1367:1390	The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
32459165	5	59	theme	genome	561:566	arg1	analysis					568:575	Subsequent whole genome analysis	544:575	Subsequent whole genome analysis	544:575	Subsequent whole genome analysis showed that the genome-to-genome distance of strain LMM-1652T to its closest relatives was in the range of 23.2-24.8 %, while the average nucleotide identity values ranged from 73.7 to 74.3%, thus confirming that this isolate represents a novel species.
32459165	4	60	theme	%	451:451	arg1	falsenii					435:442	Corynebacterium falsenii	419:442	Corynebacterium falsenii (97.05 % similarity)	419:463	16S rRNA gene sequence similarity analyses revealed that its closest relatives were Corynebacterium falsenii (97.05 % similarity), Corynebacterium jeikeium (96.83 %) and Corynebacterium urealyticum (96.82 %).
32459165	4	60	theme	%	451:451	arg1	similarity					453:462	97.05 % similarity	445:462	97.05 % similarity	445:462	16S rRNA gene sequence similarity analyses revealed that its closest relatives were Corynebacterium falsenii (97.05 % similarity), Corynebacterium jeikeium (96.83 %) and Corynebacterium urealyticum (96.82 %).
32459165	11	61	theme	Corynebacterium	1565:1579	arg1	species					1544:1550	a novel species	1536:1550	a novel species of the genus Corynebacterium with the name Corynebacterium urogenitale LMM-1652T (=LMG 31163T=DSM 108747T)	1536:1657	We propose a novel species of the genus Corynebacterium with the name Corynebacterium urogenitale LMM-1652T (=LMG 31163T=DSM 108747T).
32459165	7	62	theme	polar	935:939	arg1	profile					948:954	The polar lipids profile	931:954	The polar lipids profile of the strain	931:968	The polar lipids profile of the strain consisted mainly of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol-mannoside, as well as one unidentified lipid lacking any functional group.
32459165	3	63	dep	had	263:265	arg1	oxidase-negative					317:332	oxidase-negative	317:332	oxidase-negative	317:332	The strain had a rod to coccoid-shape, was catalase-positive and oxidase-negative.
32459165	3	63	dep	had	263:265	arg1	catalase-positive					295:311	catalase-positive	295:311	catalase-positive	295:311	The strain had a rod to coccoid-shape, was catalase-positive and oxidase-negative.
32459165	4	64	theme	97.05 	445:450	arg1	falsenii					435:442	Corynebacterium falsenii	419:442	Corynebacterium falsenii (97.05 % similarity)	419:463	16S rRNA gene sequence similarity analyses revealed that its closest relatives were Corynebacterium falsenii (97.05 % similarity), Corynebacterium jeikeium (96.83 %) and Corynebacterium urealyticum (96.82 %).
32459165	4	64	theme	97.05 	445:450	arg1	similarity					453:462	97.05 % similarity	445:462	97.05 % similarity	445:462	16S rRNA gene sequence similarity analyses revealed that its closest relatives were Corynebacterium falsenii (97.05 % similarity), Corynebacterium jeikeium (96.83 %) and Corynebacterium urealyticum (96.82 %).
32459165	7	65	theme	lipids	941:946	arg1	profile					948:954	The polar lipids profile	931:954	The polar lipids profile of the strain	931:968	The polar lipids profile of the strain consisted mainly of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol-mannoside, as well as one unidentified lipid lacking any functional group.
32459165	9	66	contain	contained	1357:1365	arg1	wall					1352:1355	The cell wall	1343:1355	The cell wall	1343:1355	The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
32459165	9	66	contain	contained	1357:1365	arg2	acid					1418:1421	the diagnostic diamino acid	1395:1421	the diagnostic diamino acid of the peptidoglycan	1395:1442	The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
32459165	9	66	contain	contained	1357:1365	arg2	acid					1387:1390	meso-diaminopimelic acid	1367:1390	meso-diaminopimelic acid	1367:1390	The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid of the peptidoglycan.
32459165	10	67	dep	C18 	1491:1494	arg1	C18 					1504:1507	C18 	1504:1507	C18 	1504:1507	The fatty acid profile was mainly composed of C18 : 1 ω9c, C18 : 0 and C16 : 0.
32459165	10	67	dep	C18 	1491:1494	arg1	 0					1521:1522	 0	1521:1522	 0	1521:1522	The fatty acid profile was mainly composed of C18 : 1 ω9c, C18 : 0 and C16 : 0.
32459165	10	67	dep	C18 	1491:1494	arg1	C16 					1516:1519	C16 	1516:1519	C16 	1516:1519	The fatty acid profile was mainly composed of C18 : 1 ω9c, C18 : 0 and C16 : 0.
32459165	10	67	dep	C18 	1491:1494	arg1	 0					1509:1510	 0	1509:1510	 0	1509:1510	The fatty acid profile was mainly composed of C18 : 1 ω9c, C18 : 0 and C16 : 0.
32459165	4	68	theme	16S	335:337	arg1	similarity					358:367	16S rRNA gene sequence similarity	335:367	16S rRNA gene sequence similarity analyses	335:376	16S rRNA gene sequence similarity analyses revealed that its closest relatives were Corynebacterium falsenii (97.05 % similarity), Corynebacterium jeikeium (96.83 %) and Corynebacterium urealyticum (96.82 %).
33238221	9	0	theme	excessive	1722:1730	arg1	loss					1732:1735	excessive loss	1722:1735	excessive loss of goblet cells	1722:1751	This endoplasmic reticulum stress response was linked to the necroptotic death of IECs, leading to excessive loss of goblet cells, formation of a thin mucus barrier, increased intestinal permeability, and infiltration of the epithelium by microbes.
33238221	7	1	from	colitis	1411:1417	arg1	mice					1449:1452	adult mice	1443:1452	adult mice	1443:1452	RESULTS Inducible loss of CTα in the intestinal epithelium reduced colonic PC concentrations and resulted in rapid and spontaneous colitis with 100% penetrance in adult mice.
33238221	7	2	theme	CTα	1306:1308	arg1	loss					1298:1301	Inducible loss	1288:1301	Inducible loss of CTα in the intestinal epithelium	1288:1337	RESULTS Inducible loss of CTα in the intestinal epithelium reduced colonic PC concentrations and resulted in rapid and spontaneous colitis with 100% penetrance in adult mice.
33238221	10	3	theme	crucial	1984:1990	arg1	role					1992:1995	a crucial role	1982:1995	a crucial role for IEC phospholipid equilibrium in colonic homeostasis	1982:2051	CONCLUSIONS Maintaining the PC content of IEC membranes protects against colitis development in mice, showing a crucial role for IEC phospholipid equilibrium in colonic homeostasis.
33238221	3	4	theme	Colonic	689:695	arg1	tissue					697:702	METHODS Colonic tissue	681:702	METHODS Colonic tissue of CTαIKO mice and control mice	681:734	METHODS Colonic tissue of CTαIKO mice and control mice was analyzed by histology, immunofluorescence, electron microscopy, quantitative polymerase chain reaction, Western blot, and thin-layer chromatography.
33238221	4	5	theme	colitis	905:911	arg1	scores					913:918	Histopathologic colitis scores	889:918	Histopathologic colitis scores	889:918	Histopathologic colitis scores were assigned by a pathologist blinded to the experimental groupings.
33238221	3	6	theme	control	723:729	arg1	mice					731:734	control mice	723:734	control mice	723:734	METHODS Colonic tissue of CTαIKO mice and control mice was analyzed by histology, immunofluorescence, electron microscopy, quantitative polymerase chain reaction, Western blot, and thin-layer chromatography.
33238221	1	7	theme	gastrointestinal	271:286	arg1	mucus					288:292	gastrointestinal mucus	271:292	gastrointestinal mucus	271:292	BACKGROUND & AIMS Patients with ulcerative colitis have low concentrations of the major membrane lipid phosphatidylcholine (PC) in gastrointestinal mucus, suggesting that defects in colonic PC metabolism might be involved in the development of colitis.
33238221	9	8	theme	cells	1747:1751	arg1	infiltration					1828:1839	infiltration	1828:1839	infiltration of the epithelium by microbes	1828:1869	This endoplasmic reticulum stress response was linked to the necroptotic death of IECs, leading to excessive loss of goblet cells, formation of a thin mucus barrier, increased intestinal permeability, and infiltration of the epithelium by microbes.
33238221	9	8	theme	cells	1747:1751	arg1	formation					1754:1762	formation	1754:1762	formation of a thin mucus barrier	1754:1786	This endoplasmic reticulum stress response was linked to the necroptotic death of IECs, leading to excessive loss of goblet cells, formation of a thin mucus barrier, increased intestinal permeability, and infiltration of the epithelium by microbes.
33238221	9	8	theme	cells	1747:1751	arg1	loss					1732:1735	excessive loss	1722:1735	excessive loss of goblet cells	1722:1751	This endoplasmic reticulum stress response was linked to the necroptotic death of IECs, leading to excessive loss of goblet cells, formation of a thin mucus barrier, increased intestinal permeability, and infiltration of the epithelium by microbes.
33238221	9	8	theme	cells	1747:1751	arg1	permeability					1810:1821	increased intestinal permeability	1789:1821	increased intestinal permeability	1789:1821	This endoplasmic reticulum stress response was linked to the necroptotic death of IECs, leading to excessive loss of goblet cells, formation of a thin mucus barrier, increased intestinal permeability, and infiltration of the epithelium by microbes.
33238221	9	9	attach	linked	1670:1675	arg1	death					1696:1700	the necroptotic death	1680:1700	the necroptotic death of IECs	1680:1708	This endoplasmic reticulum stress response was linked to the necroptotic death of IECs, leading to excessive loss of goblet cells, formation of a thin mucus barrier, increased intestinal permeability, and infiltration of the epithelium by microbes.
33238221	9	9	attach	linked	1670:1675	arg2	response					1657:1664	This endoplasmic reticulum stress response	1623:1664	This endoplasmic reticulum stress response	1623:1664	This endoplasmic reticulum stress response was linked to the necroptotic death of IECs, leading to excessive loss of goblet cells, formation of a thin mucus barrier, increased intestinal permeability, and infiltration of the epithelium by microbes.
33238221	3	10	theme	mice	714:717	arg1	tissue					697:702	METHODS Colonic tissue	681:702	METHODS Colonic tissue of CTαIKO mice and control mice	681:734	METHODS Colonic tissue of CTαIKO mice and control mice was analyzed by histology, immunofluorescence, electron microscopy, quantitative polymerase chain reaction, Western blot, and thin-layer chromatography.
33238221	8	11	from	development	1463:1473	arg1	mice					1485:1488	CTαIKO mice	1478:1488	CTαIKO mice	1478:1488	Colitis development in CTαIKO mice was traced to a severe and unresolving endoplasmic reticulum stress response in IECs with altered membrane phospholipid composition.
33238221	2	12	theme	cytidine	606:613	arg1	triphosphate					615:626	cytidine triphosphate	606:626	the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice)	542:678	To determine the precise role that PC plays in colonic barrier function, we examined mice with intestinal epithelial cell (IEC)-specific deletion of the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice).
33238221	6	13	theme	dietary	1218:1224	arg1	supplementation					1229:1243	dietary PC supplementation	1218:1243	dietary PC supplementation	1218:1243	Subsets of CTαIKO mice and control mice were treated with dietary PC supplementation, antibiotics, or 4-phenylbutyrate.
33238221	0	14	from	Colitis	123:129	arg1	Mice					134:137	Mice	134:137	Mice	134:137	Intestinal Phospholipid Disequilibrium Initiates an ER Stress Response That Drives Goblet Cell Necroptosis and Spontaneous Colitis in Mice.
33238221	1	15	theme	low	196:198	arg1	concentrations					200:213	low concentrations	196:213	low concentrations of the major membrane lipid phosphatidylcholine (PC)	196:266	BACKGROUND & AIMS Patients with ulcerative colitis have low concentrations of the major membrane lipid phosphatidylcholine (PC) in gastrointestinal mucus, suggesting that defects in colonic PC metabolism might be involved in the development of colitis.
33238221	10	16	from	role	1992:1995	arg1	homeostasis					2041:2051	colonic homeostasis	2033:2051	colonic homeostasis	2033:2051	CONCLUSIONS Maintaining the PC content of IEC membranes protects against colitis development in mice, showing a crucial role for IEC phospholipid equilibrium in colonic homeostasis.
33238221	2	17	theme	rate-limiting	546:558	arg1	enzyme					560:565	the rate-limiting enzyme	542:565	the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice)	542:678	To determine the precise role that PC plays in colonic barrier function, we examined mice with intestinal epithelial cell (IEC)-specific deletion of the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice).
33238221	10	18	theme	colonic	2033:2039	arg1	homeostasis					2041:2051	colonic homeostasis	2033:2051	colonic homeostasis	2033:2051	CONCLUSIONS Maintaining the PC content of IEC membranes protects against colitis development in mice, showing a crucial role for IEC phospholipid equilibrium in colonic homeostasis.
33238221	9	19	theme	mucus	1774:1778	arg1	barrier					1780:1786	a thin mucus barrier	1767:1786	a thin mucus barrier	1767:1786	This endoplasmic reticulum stress response was linked to the necroptotic death of IECs, leading to excessive loss of goblet cells, formation of a thin mucus barrier, increased intestinal permeability, and infiltration of the epithelium by microbes.
33238221	5	20	theme	RNA	1145:1147	arg1	amplicons					1149:1157	ribosomal RNA amplicons	1135:1157	ribosomal RNA amplicons	1135:1157	Intestinal permeability was assessed by fluorescein isothiocyanate-dextran gavage and fecal microbial composition was analyzed by sequencing 16s ribosomal RNA amplicons.
33238221	1	21	theme	major	222:226	arg1	phosphatidylcholine					243:261	the major membrane lipid phosphatidylcholine	218:261	the major membrane lipid phosphatidylcholine (PC)	218:266	BACKGROUND & AIMS Patients with ulcerative colitis have low concentrations of the major membrane lipid phosphatidylcholine (PC) in gastrointestinal mucus, suggesting that defects in colonic PC metabolism might be involved in the development of colitis.
33238221	1	21	theme	major	222:226	arg1	PC					264:265	PC	264:265	PC	264:265	BACKGROUND & AIMS Patients with ulcerative colitis have low concentrations of the major membrane lipid phosphatidylcholine (PC) in gastrointestinal mucus, suggesting that defects in colonic PC metabolism might be involved in the development of colitis.
33238221	2	22	theme	major	574:578	arg1	pathway					580:586	the major pathway	570:586	the major pathway	570:586	To determine the precise role that PC plays in colonic barrier function, we examined mice with intestinal epithelial cell (IEC)-specific deletion of the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice).
33238221	7	23	theme	colonic	1347:1353	arg1	concentrations					1358:1371	colonic PC concentrations	1347:1371	colonic PC concentrations	1347:1371	RESULTS Inducible loss of CTα in the intestinal epithelium reduced colonic PC concentrations and resulted in rapid and spontaneous colitis with 100% penetrance in adult mice.
33238221	9	24	theme	intestinal	1799:1808	arg1	permeability					1810:1821	increased intestinal permeability	1789:1821	increased intestinal permeability	1789:1821	This endoplasmic reticulum stress response was linked to the necroptotic death of IECs, leading to excessive loss of goblet cells, formation of a thin mucus barrier, increased intestinal permeability, and infiltration of the epithelium by microbes.
33238221	8	25	theme	endoplasmic	1529:1539	arg1	reticulum					1541:1549	a severe and unresolving endoplasmic reticulum	1504:1549	a severe and unresolving endoplasmic reticulum stress response in IECs with altered membrane phospholipid composition	1504:1620	Colitis development in CTαIKO mice was traced to a severe and unresolving endoplasmic reticulum stress response in IECs with altered membrane phospholipid composition.
33238221	7	26	theme	%	1427:1427	arg1	penetrance					1429:1438	100% penetrance	1424:1438	100% penetrance	1424:1438	RESULTS Inducible loss of CTα in the intestinal epithelium reduced colonic PC concentrations and resulted in rapid and spontaneous colitis with 100% penetrance in adult mice.
33238221	3	27	theme	quantitative	804:815	arg1	reaction					834:841	quantitative polymerase chain reaction	804:841	quantitative polymerase chain reaction	804:841	METHODS Colonic tissue of CTαIKO mice and control mice was analyzed by histology, immunofluorescence, electron microscopy, quantitative polymerase chain reaction, Western blot, and thin-layer chromatography.
33238221	7	28	with	colitis	1411:1417	arg1	penetrance					1429:1438	100% penetrance	1424:1438	100% penetrance	1424:1438	RESULTS Inducible loss of CTα in the intestinal epithelium reduced colonic PC concentrations and resulted in rapid and spontaneous colitis with 100% penetrance in adult mice.
33238221	1	29	theme	BACKGROUND	140:149	arg1	AIMS					153:156	BACKGROUND & AIMS	140:156	BACKGROUND & AIMS Patients with ulcerative colitis have low concentrations of the major membrane lipid phosphatidylcholine (PC) in gastrointestinal mucus, suggesting that defects in colonic PC metabolism might be involved in the development of colitis.	140:391	BACKGROUND & AIMS Patients with ulcerative colitis have low concentrations of the major membrane lipid phosphatidylcholine (PC) in gastrointestinal mucus, suggesting that defects in colonic PC metabolism might be involved in the development of colitis.
33238221	8	30	theme	stress	1551:1556	arg1	response					1558:1565	a severe and unresolving endoplasmic reticulum stress response	1504:1565	a severe and unresolving endoplasmic reticulum stress response in IECs with altered membrane phospholipid composition	1504:1620	Colitis development in CTαIKO mice was traced to a severe and unresolving endoplasmic reticulum stress response in IECs with altered membrane phospholipid composition.
33238221	10	31	theme	PC	1900:1901	arg1	content					1903:1909	the PC content	1896:1909	the PC content of IEC membranes	1896:1926	CONCLUSIONS Maintaining the PC content of IEC membranes protects against colitis development in mice, showing a crucial role for IEC phospholipid equilibrium in colonic homeostasis.
33238221	2	32	with	mice	478:481	arg1	deletion					530:537	intestinal epithelial cell (IEC)-specific deletion	488:537	intestinal epithelial cell (IEC)-specific deletion of the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice)	488:678	To determine the precise role that PC plays in colonic barrier function, we examined mice with intestinal epithelial cell (IEC)-specific deletion of the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice).
33238221	2	33	theme	intestinal	488:497	arg1	IEC					516:518	IEC	516:518	IEC	516:518	To determine the precise role that PC plays in colonic barrier function, we examined mice with intestinal epithelial cell (IEC)-specific deletion of the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice).
33238221	2	33	theme	intestinal	488:497	arg1	cell					510:513	intestinal epithelial cell	488:513	intestinal epithelial cell (IEC)	488:519	To determine the precise role that PC plays in colonic barrier function, we examined mice with intestinal epithelial cell (IEC)-specific deletion of the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice).
33238221	0	34	theme	Goblet	83:88	arg1	Necroptosis					95:105	Goblet Cell Necroptosis	83:105	Goblet Cell Necroptosis	83:105	Intestinal Phospholipid Disequilibrium Initiates an ER Stress Response That Drives Goblet Cell Necroptosis and Spontaneous Colitis in Mice.
33238221	9	35	theme	goblet	1740:1745	arg1	cells					1747:1751	goblet cells	1740:1751	goblet cells	1740:1751	This endoplasmic reticulum stress response was linked to the necroptotic death of IECs, leading to excessive loss of goblet cells, formation of a thin mucus barrier, increased intestinal permeability, and infiltration of the epithelium by microbes.
33238221	2	36	theme	-specific	520:528	arg1	deletion					530:537	intestinal epithelial cell (IEC)-specific deletion	488:537	intestinal epithelial cell (IEC)-specific deletion of the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice)	488:678	To determine the precise role that PC plays in colonic barrier function, we examined mice with intestinal epithelial cell (IEC)-specific deletion of the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice).
33238221	3	37	theme	chain	828:832	arg1	reaction					834:841	quantitative polymerase chain reaction	804:841	quantitative polymerase chain reaction	804:841	METHODS Colonic tissue of CTαIKO mice and control mice was analyzed by histology, immunofluorescence, electron microscopy, quantitative polymerase chain reaction, Western blot, and thin-layer chromatography.
33238221	9	38	theme	endoplasmic	1628:1638	arg1	reticulum					1640:1648	endoplasmic reticulum	1628:1648	This endoplasmic reticulum stress response	1623:1664	This endoplasmic reticulum stress response was linked to the necroptotic death of IECs, leading to excessive loss of goblet cells, formation of a thin mucus barrier, increased intestinal permeability, and infiltration of the epithelium by microbes.
33238221	8	39	theme	membrane	1588:1595	arg1	composition					1610:1620	altered membrane phospholipid composition	1580:1620	altered membrane phospholipid composition	1580:1620	Colitis development in CTαIKO mice was traced to a severe and unresolving endoplasmic reticulum stress response in IECs with altered membrane phospholipid composition.
33238221	2	40	theme	phosphocholine	628:641	arg1	cytidylyltransferase-α					643:664	phosphocholine cytidylyltransferase-α	628:664	the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice)	542:678	To determine the precise role that PC plays in colonic barrier function, we examined mice with intestinal epithelial cell (IEC)-specific deletion of the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice).
33238221	2	40	theme	phosphocholine	628:641	arg1	mice					674:677	CTαIKO mice	667:677	CTαIKO mice	667:677	To determine the precise role that PC plays in colonic barrier function, we examined mice with intestinal epithelial cell (IEC)-specific deletion of the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice).
33238221	0	41	theme	Spontaneous	111:121	arg1	Colitis					123:129	Spontaneous Colitis	111:129	Spontaneous Colitis	111:129	Intestinal Phospholipid Disequilibrium Initiates an ER Stress Response That Drives Goblet Cell Necroptosis and Spontaneous Colitis in Mice.
33238221	9	42	theme	stress	1650:1655	arg1	response					1657:1664	This endoplasmic reticulum stress response	1623:1664	This endoplasmic reticulum stress response	1623:1664	This endoplasmic reticulum stress response was linked to the necroptotic death of IECs, leading to excessive loss of goblet cells, formation of a thin mucus barrier, increased intestinal permeability, and infiltration of the epithelium by microbes.
33238221	10	43	theme	phospholipid	2005:2016	arg1	equilibrium					2018:2028	IEC phospholipid equilibrium	2001:2028	IEC phospholipid equilibrium	2001:2028	CONCLUSIONS Maintaining the PC content of IEC membranes protects against colitis development in mice, showing a crucial role for IEC phospholipid equilibrium in colonic homeostasis.
33238221	8	44	with	IECs	1570:1573	arg1	composition					1610:1620	altered membrane phospholipid composition	1580:1620	altered membrane phospholipid composition	1580:1620	Colitis development in CTαIKO mice was traced to a severe and unresolving endoplasmic reticulum stress response in IECs with altered membrane phospholipid composition.
33238221	0	45	theme	Intestinal	0:9	arg1	Disequilibrium					24:37	Intestinal Phospholipid Disequilibrium	0:37	Intestinal Phospholipid Disequilibrium	0:37	Intestinal Phospholipid Disequilibrium Initiates an ER Stress Response That Drives Goblet Cell Necroptosis and Spontaneous Colitis in Mice.
33238221	0	46	from	Necroptosis	95:105	arg1	Mice					134:137	Mice	134:137	Mice	134:137	Intestinal Phospholipid Disequilibrium Initiates an ER Stress Response That Drives Goblet Cell Necroptosis and Spontaneous Colitis in Mice.
33238221	2	47	from	pathway	580:586	arg1	deletion					530:537	intestinal epithelial cell (IEC)-specific deletion	488:537	intestinal epithelial cell (IEC)-specific deletion of the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice)	488:678	To determine the precise role that PC plays in colonic barrier function, we examined mice with intestinal epithelial cell (IEC)-specific deletion of the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice).
33238221	7	48	dep	RESULTS	1280:1286	arg1	resulted					1377:1384	resulted	1377:1384	resulted in rapid and spontaneous colitis with 100% penetrance in adult mice	1377:1452	RESULTS Inducible loss of CTα in the intestinal epithelium reduced colonic PC concentrations and resulted in rapid and spontaneous colitis with 100% penetrance in adult mice.
33238221	7	48	dep	RESULTS	1280:1286	arg1	reduced					1339:1345	reduced	1339:1345	reduced colonic PC concentrations	1339:1371	RESULTS Inducible loss of CTα in the intestinal epithelium reduced colonic PC concentrations and resulted in rapid and spontaneous colitis with 100% penetrance in adult mice.
33238221	1	49	contain	have	191:194	arg1	Patients					158:165	Patients	158:165	Patients with ulcerative colitis	158:189	BACKGROUND & AIMS Patients with ulcerative colitis have low concentrations of the major membrane lipid phosphatidylcholine (PC) in gastrointestinal mucus, suggesting that defects in colonic PC metabolism might be involved in the development of colitis.
33238221	1	49	contain	have	191:194	arg2	concentrations					200:213	low concentrations	196:213	low concentrations of the major membrane lipid phosphatidylcholine (PC)	196:266	BACKGROUND & AIMS Patients with ulcerative colitis have low concentrations of the major membrane lipid phosphatidylcholine (PC) in gastrointestinal mucus, suggesting that defects in colonic PC metabolism might be involved in the development of colitis.
33238221	5	50	theme	fecal	1076:1080	arg1	composition					1092:1102	fecal microbial composition	1076:1102	fecal microbial composition	1076:1102	Intestinal permeability was assessed by fluorescein isothiocyanate-dextran gavage and fecal microbial composition was analyzed by sequencing 16s ribosomal RNA amplicons.
33238221	10	51	theme	membranes	1918:1926	arg1	content					1903:1909	the PC content	1896:1909	the PC content of IEC membranes	1896:1926	CONCLUSIONS Maintaining the PC content of IEC membranes protects against colitis development in mice, showing a crucial role for IEC phospholipid equilibrium in colonic homeostasis.
33238221	8	52	theme	CTαIKO	1478:1483	arg1	mice					1485:1488	CTαIKO mice	1478:1488	CTαIKO mice	1478:1488	Colitis development in CTαIKO mice was traced to a severe and unresolving endoplasmic reticulum stress response in IECs with altered membrane phospholipid composition.
33238221	10	53	theme	IEC	2001:2003	arg1	equilibrium					2018:2028	IEC phospholipid equilibrium	2001:2028	IEC phospholipid equilibrium	2001:2028	CONCLUSIONS Maintaining the PC content of IEC membranes protects against colitis development in mice, showing a crucial role for IEC phospholipid equilibrium in colonic homeostasis.
33238221	0	54	theme	Stress	55:60	arg1	Response					62:69	an ER Stress Response	49:69	an ER Stress Response That Drives Goblet Cell Necroptosis and Spontaneous Colitis in Mice	49:137	Intestinal Phospholipid Disequilibrium Initiates an ER Stress Response That Drives Goblet Cell Necroptosis and Spontaneous Colitis in Mice.
33238221	1	55	theme	PC	330:331	arg1	metabolism					333:342	PC metabolism	330:342	PC metabolism	330:342	BACKGROUND & AIMS Patients with ulcerative colitis have low concentrations of the major membrane lipid phosphatidylcholine (PC) in gastrointestinal mucus, suggesting that defects in colonic PC metabolism might be involved in the development of colitis.
33238221	7	56	theme	PC	1355:1356	arg1	concentrations					1358:1371	colonic PC concentrations	1347:1371	colonic PC concentrations	1347:1371	RESULTS Inducible loss of CTα in the intestinal epithelium reduced colonic PC concentrations and resulted in rapid and spontaneous colitis with 100% penetrance in adult mice.
33238221	11	57	theme	accession	2058:2066	arg1	number					2068:2073	SRA accession number	2054:2073	SRA accession number	2054:2073	SRA accession number: PRJNA562603.
33238221	3	58	theme	METHODS	681:687	arg1	tissue					697:702	METHODS Colonic tissue	681:702	METHODS Colonic tissue of CTαIKO mice and control mice	681:734	METHODS Colonic tissue of CTαIKO mice and control mice was analyzed by histology, immunofluorescence, electron microscopy, quantitative polymerase chain reaction, Western blot, and thin-layer chromatography.
33238221	9	59	theme	necroptotic	1684:1694	arg1	death					1696:1700	the necroptotic death	1680:1700	the necroptotic death of IECs	1680:1708	This endoplasmic reticulum stress response was linked to the necroptotic death of IECs, leading to excessive loss of goblet cells, formation of a thin mucus barrier, increased intestinal permeability, and infiltration of the epithelium by microbes.
33238221	1	60	theme	membrane	228:235	arg1	phosphatidylcholine					243:261	the major membrane lipid phosphatidylcholine	218:261	the major membrane lipid phosphatidylcholine (PC)	218:266	BACKGROUND & AIMS Patients with ulcerative colitis have low concentrations of the major membrane lipid phosphatidylcholine (PC) in gastrointestinal mucus, suggesting that defects in colonic PC metabolism might be involved in the development of colitis.
33238221	1	60	theme	membrane	228:235	arg1	PC					264:265	PC	264:265	PC	264:265	BACKGROUND & AIMS Patients with ulcerative colitis have low concentrations of the major membrane lipid phosphatidylcholine (PC) in gastrointestinal mucus, suggesting that defects in colonic PC metabolism might be involved in the development of colitis.
33238221	5	61	theme	fluorescein	1030:1040	arg1	isothiocyanate-dextran					1042:1063	fluorescein isothiocyanate-dextran	1030:1063	fluorescein isothiocyanate-dextran gavage	1030:1070	Intestinal permeability was assessed by fluorescein isothiocyanate-dextran gavage and fecal microbial composition was analyzed by sequencing 16s ribosomal RNA amplicons.
33238221	6	62	theme	mice	1178:1181	arg1	Subsets					1160:1166	Subsets	1160:1166	Subsets of CTαIKO mice and control mice	1160:1198	Subsets of CTαIKO mice and control mice were treated with dietary PC supplementation, antibiotics, or 4-phenylbutyrate.
33238221	2	63	from	deletion	530:537	arg1	pathway					580:586	the major pathway	570:586	the major pathway	570:586	To determine the precise role that PC plays in colonic barrier function, we examined mice with intestinal epithelial cell (IEC)-specific deletion of the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice).
33238221	1	64	theme	phosphatidylcholine	243:261	arg1	concentrations					200:213	low concentrations	196:213	low concentrations of the major membrane lipid phosphatidylcholine (PC)	196:266	BACKGROUND & AIMS Patients with ulcerative colitis have low concentrations of the major membrane lipid phosphatidylcholine (PC) in gastrointestinal mucus, suggesting that defects in colonic PC metabolism might be involved in the development of colitis.
33238221	2	65	dep	enzyme	560:565	arg1	triphosphate					615:626	cytidine triphosphate	606:626	the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice)	542:678	To determine the precise role that PC plays in colonic barrier function, we examined mice with intestinal epithelial cell (IEC)-specific deletion of the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice).
33238221	2	65	dep	enzyme	560:565	arg1	cytidylyltransferase-α					643:664	phosphocholine cytidylyltransferase-α	628:664	the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice)	542:678	To determine the precise role that PC plays in colonic barrier function, we examined mice with intestinal epithelial cell (IEC)-specific deletion of the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice).
33238221	2	65	dep	enzyme	560:565	arg1	mice					674:677	CTαIKO mice	667:677	CTαIKO mice	667:677	To determine the precise role that PC plays in colonic barrier function, we examined mice with intestinal epithelial cell (IEC)-specific deletion of the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice).
33238221	7	66	theme	intestinal	1317:1326	arg1	epithelium					1328:1337	the intestinal epithelium	1313:1337	the intestinal epithelium	1313:1337	RESULTS Inducible loss of CTα in the intestinal epithelium reduced colonic PC concentrations and resulted in rapid and spontaneous colitis with 100% penetrance in adult mice.
33238221	3	67	theme	CTαIKO	707:712	arg1	mice					714:717	CTαIKO mice	707:717	CTαIKO mice	707:717	METHODS Colonic tissue of CTαIKO mice and control mice was analyzed by histology, immunofluorescence, electron microscopy, quantitative polymerase chain reaction, Western blot, and thin-layer chromatography.
33238221	2	68	theme	PC	592:593	arg1	synthesis					595:603	PC synthesis	592:603	PC synthesis	592:603	To determine the precise role that PC plays in colonic barrier function, we examined mice with intestinal epithelial cell (IEC)-specific deletion of the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice).
33238221	2	69	from	enzyme	560:565	arg1	pathway					580:586	the major pathway	570:586	the major pathway	570:586	To determine the precise role that PC plays in colonic barrier function, we examined mice with intestinal epithelial cell (IEC)-specific deletion of the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice).
33238221	10	70	from	development	1953:1963	arg1	mice					1968:1971	mice	1968:1971	mice	1968:1971	CONCLUSIONS Maintaining the PC content of IEC membranes protects against colitis development in mice, showing a crucial role for IEC phospholipid equilibrium in colonic homeostasis.
33238221	4	71	theme	Histopathologic	889:903	arg1	scores					913:918	Histopathologic colitis scores	889:918	Histopathologic colitis scores	889:918	Histopathologic colitis scores were assigned by a pathologist blinded to the experimental groupings.
33238221	3	72	theme	mice	731:734	arg1	tissue					697:702	METHODS Colonic tissue	681:702	METHODS Colonic tissue of CTαIKO mice and control mice	681:734	METHODS Colonic tissue of CTαIKO mice and control mice was analyzed by histology, immunofluorescence, electron microscopy, quantitative polymerase chain reaction, Western blot, and thin-layer chromatography.
33238221	1	73	theme	ulcerative	172:181	arg1	colitis					183:189	ulcerative colitis	172:189	ulcerative colitis	172:189	BACKGROUND & AIMS Patients with ulcerative colitis have low concentrations of the major membrane lipid phosphatidylcholine (PC) in gastrointestinal mucus, suggesting that defects in colonic PC metabolism might be involved in the development of colitis.
33238221	6	74	theme	PC	1226:1227	arg1	supplementation					1229:1243	dietary PC supplementation	1218:1243	dietary PC supplementation	1218:1243	Subsets of CTαIKO mice and control mice were treated with dietary PC supplementation, antibiotics, or 4-phenylbutyrate.
33238221	7	75	theme	Inducible	1288:1296	arg1	loss					1298:1301	Inducible loss	1288:1301	Inducible loss of CTα in the intestinal epithelium	1288:1337	RESULTS Inducible loss of CTα in the intestinal epithelium reduced colonic PC concentrations and resulted in rapid and spontaneous colitis with 100% penetrance in adult mice.
33238221	9	76	theme	thin	1769:1772	arg1	barrier					1780:1786	a thin mucus barrier	1767:1786	a thin mucus barrier	1767:1786	This endoplasmic reticulum stress response was linked to the necroptotic death of IECs, leading to excessive loss of goblet cells, formation of a thin mucus barrier, increased intestinal permeability, and infiltration of the epithelium by microbes.
33238221	8	77	theme	severe	1506:1511	arg1	reticulum					1541:1549	a severe and unresolving endoplasmic reticulum	1504:1549	a severe and unresolving endoplasmic reticulum stress response in IECs with altered membrane phospholipid composition	1504:1620	Colitis development in CTαIKO mice was traced to a severe and unresolving endoplasmic reticulum stress response in IECs with altered membrane phospholipid composition.
33238221	6	78	theme	control	1187:1193	arg1	mice					1195:1198	control mice	1187:1198	control mice	1187:1198	Subsets of CTαIKO mice and control mice were treated with dietary PC supplementation, antibiotics, or 4-phenylbutyrate.
33238221	5	79	theme	ribosomal	1135:1143	arg1	amplicons					1149:1157	ribosomal RNA amplicons	1135:1157	ribosomal RNA amplicons	1135:1157	Intestinal permeability was assessed by fluorescein isothiocyanate-dextran gavage and fecal microbial composition was analyzed by sequencing 16s ribosomal RNA amplicons.
33238221	9	80	theme	barrier	1780:1786	arg1	infiltration					1828:1839	infiltration	1828:1839	infiltration of the epithelium by microbes	1828:1869	This endoplasmic reticulum stress response was linked to the necroptotic death of IECs, leading to excessive loss of goblet cells, formation of a thin mucus barrier, increased intestinal permeability, and infiltration of the epithelium by microbes.
33238221	9	80	theme	barrier	1780:1786	arg1	formation					1754:1762	formation	1754:1762	formation of a thin mucus barrier	1754:1786	This endoplasmic reticulum stress response was linked to the necroptotic death of IECs, leading to excessive loss of goblet cells, formation of a thin mucus barrier, increased intestinal permeability, and infiltration of the epithelium by microbes.
33238221	9	80	theme	barrier	1780:1786	arg1	loss					1732:1735	excessive loss	1722:1735	excessive loss of goblet cells	1722:1751	This endoplasmic reticulum stress response was linked to the necroptotic death of IECs, leading to excessive loss of goblet cells, formation of a thin mucus barrier, increased intestinal permeability, and infiltration of the epithelium by microbes.
33238221	9	80	theme	barrier	1780:1786	arg1	permeability					1810:1821	increased intestinal permeability	1789:1821	increased intestinal permeability	1789:1821	This endoplasmic reticulum stress response was linked to the necroptotic death of IECs, leading to excessive loss of goblet cells, formation of a thin mucus barrier, increased intestinal permeability, and infiltration of the epithelium by microbes.
33238221	8	81	theme	unresolving	1517:1527	arg1	reticulum					1541:1549	a severe and unresolving endoplasmic reticulum	1504:1549	a severe and unresolving endoplasmic reticulum stress response in IECs with altered membrane phospholipid composition	1504:1620	Colitis development in CTαIKO mice was traced to a severe and unresolving endoplasmic reticulum stress response in IECs with altered membrane phospholipid composition.
33238221	4	82	theme	experimental	966:977	arg1	groupings					979:987	the experimental groupings	962:987	the experimental groupings	962:987	Histopathologic colitis scores were assigned by a pathologist blinded to the experimental groupings.
33238221	11	83	dep	PRJNA562603	2076:2086	arg1	number					2068:2073	SRA accession number	2054:2073	SRA accession number	2054:2073	SRA accession number: PRJNA562603.
33238221	9	84	theme	increased	1789:1797	arg1	permeability					1810:1821	increased intestinal permeability	1789:1821	increased intestinal permeability	1789:1821	This endoplasmic reticulum stress response was linked to the necroptotic death of IECs, leading to excessive loss of goblet cells, formation of a thin mucus barrier, increased intestinal permeability, and infiltration of the epithelium by microbes.
33238221	8	85	theme	reticulum	1541:1549	arg1	response					1558:1565	a severe and unresolving endoplasmic reticulum stress response	1504:1565	a severe and unresolving endoplasmic reticulum stress response in IECs with altered membrane phospholipid composition	1504:1620	Colitis development in CTαIKO mice was traced to a severe and unresolving endoplasmic reticulum stress response in IECs with altered membrane phospholipid composition.
33238221	7	86	theme	adult	1443:1447	arg1	mice					1449:1452	adult mice	1443:1452	adult mice	1443:1452	RESULTS Inducible loss of CTα in the intestinal epithelium reduced colonic PC concentrations and resulted in rapid and spontaneous colitis with 100% penetrance in adult mice.
33238221	10	87	theme	IEC	1914:1916	arg1	membranes					1918:1926	IEC membranes	1914:1926	IEC membranes	1914:1926	CONCLUSIONS Maintaining the PC content of IEC membranes protects against colitis development in mice, showing a crucial role for IEC phospholipid equilibrium in colonic homeostasis.
33238221	3	88	theme	thin-layer	862:871	arg1	chromatography					873:886	thin-layer chromatography	862:886	thin-layer chromatography	862:886	METHODS Colonic tissue of CTαIKO mice and control mice was analyzed by histology, immunofluorescence, electron microscopy, quantitative polymerase chain reaction, Western blot, and thin-layer chromatography.
33238221	2	89	theme	enzyme	560:565	arg1	deletion					530:537	intestinal epithelial cell (IEC)-specific deletion	488:537	intestinal epithelial cell (IEC)-specific deletion of the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice)	488:678	To determine the precise role that PC plays in colonic barrier function, we examined mice with intestinal epithelial cell (IEC)-specific deletion of the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice).
33238221	7	90	from	loss	1298:1301	arg1	epithelium					1328:1337	the intestinal epithelium	1313:1337	the intestinal epithelium	1313:1337	RESULTS Inducible loss of CTα in the intestinal epithelium reduced colonic PC concentrations and resulted in rapid and spontaneous colitis with 100% penetrance in adult mice.
33238221	3	91	theme	polymerase	817:826	arg1	reaction					834:841	quantitative polymerase chain reaction	804:841	quantitative polymerase chain reaction	804:841	METHODS Colonic tissue of CTαIKO mice and control mice was analyzed by histology, immunofluorescence, electron microscopy, quantitative polymerase chain reaction, Western blot, and thin-layer chromatography.
33238221	11	92	theme	SRA	2054:2056	arg1	number					2068:2073	SRA accession number	2054:2073	SRA accession number	2054:2073	SRA accession number: PRJNA562603.
33238221	1	93	theme	&	151:151	arg1	AIMS					153:156	BACKGROUND & AIMS	140:156	BACKGROUND & AIMS Patients with ulcerative colitis have low concentrations of the major membrane lipid phosphatidylcholine (PC) in gastrointestinal mucus, suggesting that defects in colonic PC metabolism might be involved in the development of colitis.	140:391	BACKGROUND & AIMS Patients with ulcerative colitis have low concentrations of the major membrane lipid phosphatidylcholine (PC) in gastrointestinal mucus, suggesting that defects in colonic PC metabolism might be involved in the development of colitis.
33238221	2	94	theme	epithelial	499:508	arg1	IEC					516:518	IEC	516:518	IEC	516:518	To determine the precise role that PC plays in colonic barrier function, we examined mice with intestinal epithelial cell (IEC)-specific deletion of the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice).
33238221	2	94	theme	epithelial	499:508	arg1	cell					510:513	intestinal epithelial cell	488:513	intestinal epithelial cell (IEC)	488:519	To determine the precise role that PC plays in colonic barrier function, we examined mice with intestinal epithelial cell (IEC)-specific deletion of the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice).
33238221	7	95	theme	100	1424:1426	arg1	%					1427:1427	%	1427:1427	%	1427:1427	RESULTS Inducible loss of CTα in the intestinal epithelium reduced colonic PC concentrations and resulted in rapid and spontaneous colitis with 100% penetrance in adult mice.
33238221	0	96	theme	Cell	90:93	arg1	Necroptosis					95:105	Goblet Cell Necroptosis	83:105	Goblet Cell Necroptosis	83:105	Intestinal Phospholipid Disequilibrium Initiates an ER Stress Response That Drives Goblet Cell Necroptosis and Spontaneous Colitis in Mice.
33238221	1	97	with	Patients	158:165	arg1	colitis					183:189	ulcerative colitis	172:189	ulcerative colitis	172:189	BACKGROUND & AIMS Patients with ulcerative colitis have low concentrations of the major membrane lipid phosphatidylcholine (PC) in gastrointestinal mucus, suggesting that defects in colonic PC metabolism might be involved in the development of colitis.
33238221	8	98	theme	altered	1580:1586	arg1	composition					1610:1620	altered membrane phospholipid composition	1580:1620	altered membrane phospholipid composition	1580:1620	Colitis development in CTαIKO mice was traced to a severe and unresolving endoplasmic reticulum stress response in IECs with altered membrane phospholipid composition.
33238221	2	99	theme	CTαIKO	667:672	arg1	cytidylyltransferase-α					643:664	phosphocholine cytidylyltransferase-α	628:664	the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice)	542:678	To determine the precise role that PC plays in colonic barrier function, we examined mice with intestinal epithelial cell (IEC)-specific deletion of the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice).
33238221	2	99	theme	CTαIKO	667:672	arg1	mice					674:677	CTαIKO mice	667:677	CTαIKO mice	667:677	To determine the precise role that PC plays in colonic barrier function, we examined mice with intestinal epithelial cell (IEC)-specific deletion of the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice).
33238221	3	100	theme	Western	844:850	arg1	blot					852:855	Western blot	844:855	Western blot	844:855	METHODS Colonic tissue of CTαIKO mice and control mice was analyzed by histology, immunofluorescence, electron microscopy, quantitative polymerase chain reaction, Western blot, and thin-layer chromatography.
33238221	8	101	from	response	1558:1565	arg1	IECs					1570:1573	IECs	1570:1573	IECs with altered membrane phospholipid composition	1570:1620	Colitis development in CTαIKO mice was traced to a severe and unresolving endoplasmic reticulum stress response in IECs with altered membrane phospholipid composition.
33238221	5	102	theme	Intestinal	990:999	arg1	permeability					1001:1012	Intestinal permeability	990:1012	Intestinal permeability	990:1012	Intestinal permeability was assessed by fluorescein isothiocyanate-dextran gavage and fecal microbial composition was analyzed by sequencing 16s ribosomal RNA amplicons.
33238221	9	103	theme	epithelium	1848:1857	arg1	infiltration					1828:1839	infiltration	1828:1839	infiltration of the epithelium by microbes	1828:1869	This endoplasmic reticulum stress response was linked to the necroptotic death of IECs, leading to excessive loss of goblet cells, formation of a thin mucus barrier, increased intestinal permeability, and infiltration of the epithelium by microbes.
33238221	9	103	theme	epithelium	1848:1857	arg1	formation					1754:1762	formation	1754:1762	formation of a thin mucus barrier	1754:1786	This endoplasmic reticulum stress response was linked to the necroptotic death of IECs, leading to excessive loss of goblet cells, formation of a thin mucus barrier, increased intestinal permeability, and infiltration of the epithelium by microbes.
33238221	9	103	theme	epithelium	1848:1857	arg1	loss					1732:1735	excessive loss	1722:1735	excessive loss of goblet cells	1722:1751	This endoplasmic reticulum stress response was linked to the necroptotic death of IECs, leading to excessive loss of goblet cells, formation of a thin mucus barrier, increased intestinal permeability, and infiltration of the epithelium by microbes.
33238221	9	103	theme	epithelium	1848:1857	arg1	permeability					1810:1821	increased intestinal permeability	1789:1821	increased intestinal permeability	1789:1821	This endoplasmic reticulum stress response was linked to the necroptotic death of IECs, leading to excessive loss of goblet cells, formation of a thin mucus barrier, increased intestinal permeability, and infiltration of the epithelium by microbes.
33238221	8	104	theme	phospholipid	1597:1608	arg1	composition					1610:1620	altered membrane phospholipid composition	1580:1620	altered membrane phospholipid composition	1580:1620	Colitis development in CTαIKO mice was traced to a severe and unresolving endoplasmic reticulum stress response in IECs with altered membrane phospholipid composition.
33238221	9	105	theme	reticulum	1640:1648	arg1	response					1657:1664	This endoplasmic reticulum stress response	1623:1664	This endoplasmic reticulum stress response	1623:1664	This endoplasmic reticulum stress response was linked to the necroptotic death of IECs, leading to excessive loss of goblet cells, formation of a thin mucus barrier, increased intestinal permeability, and infiltration of the epithelium by microbes.
33238221	7	106	theme	spontaneous	1399:1409	arg1	colitis					1411:1417	rapid and spontaneous colitis	1389:1417	rapid and spontaneous colitis with 100% penetrance in adult mice	1389:1452	RESULTS Inducible loss of CTα in the intestinal epithelium reduced colonic PC concentrations and resulted in rapid and spontaneous colitis with 100% penetrance in adult mice.
33238221	0	107	theme	Phospholipid	11:22	arg1	Disequilibrium					24:37	Intestinal Phospholipid Disequilibrium	0:37	Intestinal Phospholipid Disequilibrium	0:37	Intestinal Phospholipid Disequilibrium Initiates an ER Stress Response That Drives Goblet Cell Necroptosis and Spontaneous Colitis in Mice.
33238221	1	108	theme	colitis	384:390	arg1	development					369:379	the development	365:379	the development of colitis	365:390	BACKGROUND & AIMS Patients with ulcerative colitis have low concentrations of the major membrane lipid phosphatidylcholine (PC) in gastrointestinal mucus, suggesting that defects in colonic PC metabolism might be involved in the development of colitis.
33238221	7	109	theme	rapid	1389:1393	arg1	colitis					1411:1417	rapid and spontaneous colitis	1389:1417	rapid and spontaneous colitis with 100% penetrance in adult mice	1389:1452	RESULTS Inducible loss of CTα in the intestinal epithelium reduced colonic PC concentrations and resulted in rapid and spontaneous colitis with 100% penetrance in adult mice.
33238221	2	110	theme	colonic barrier	440:454	arg1	function					456:463	colonic barrier function	440:463	colonic barrier function	440:463	To determine the precise role that PC plays in colonic barrier function, we examined mice with intestinal epithelial cell (IEC)-specific deletion of the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice).
33238221	10	111	theme	colitis	1945:1951	arg1	development					1953:1963	colitis development	1945:1963	colitis development in mice	1945:1971	CONCLUSIONS Maintaining the PC content of IEC membranes protects against colitis development in mice, showing a crucial role for IEC phospholipid equilibrium in colonic homeostasis.
33238221	1	112	dep	AIMS	153:156	arg1	have					191:194	have	191:194	have	191:194	BACKGROUND & AIMS Patients with ulcerative colitis have low concentrations of the major membrane lipid phosphatidylcholine (PC) in gastrointestinal mucus, suggesting that defects in colonic PC metabolism might be involved in the development of colitis.
33238221	0	113	theme	ER	52:53	arg1	Response					62:69	an ER Stress Response	49:69	an ER Stress Response That Drives Goblet Cell Necroptosis and Spontaneous Colitis in Mice	49:137	Intestinal Phospholipid Disequilibrium Initiates an ER Stress Response That Drives Goblet Cell Necroptosis and Spontaneous Colitis in Mice.
33238221	8	114	theme	Colitis	1455:1461	arg1	development					1463:1473	Colitis development	1455:1473	Colitis development in CTαIKO mice	1455:1488	Colitis development in CTαIKO mice was traced to a severe and unresolving endoplasmic reticulum stress response in IECs with altered membrane phospholipid composition.
33238221	2	115	theme	precise	410:416	arg1	role					418:421	the precise role that PC plays in colonic barrier function	406:463	the precise role that PC plays in colonic barrier function	406:463	To determine the precise role that PC plays in colonic barrier function, we examined mice with intestinal epithelial cell (IEC)-specific deletion of the rate-limiting enzyme in the major pathway for PC synthesis: cytidine triphosphate:phosphocholine cytidylyltransferase-α (CTαIKO mice).
33238221	5	116	theme	microbial	1082:1090	arg1	composition					1092:1102	fecal microbial composition	1076:1102	fecal microbial composition	1076:1102	Intestinal permeability was assessed by fluorescein isothiocyanate-dextran gavage and fecal microbial composition was analyzed by sequencing 16s ribosomal RNA amplicons.
33238221	6	117	theme	mice	1195:1198	arg1	Subsets					1160:1166	Subsets	1160:1166	Subsets of CTαIKO mice and control mice	1160:1198	Subsets of CTαIKO mice and control mice were treated with dietary PC supplementation, antibiotics, or 4-phenylbutyrate.
33238221	9	118	theme	IECs	1705:1708	arg1	death					1696:1700	the necroptotic death	1680:1700	the necroptotic death of IECs	1680:1708	This endoplasmic reticulum stress response was linked to the necroptotic death of IECs, leading to excessive loss of goblet cells, formation of a thin mucus barrier, increased intestinal permeability, and infiltration of the epithelium by microbes.
33238221	1	119	theme	lipid	237:241	arg1	phosphatidylcholine					243:261	the major membrane lipid phosphatidylcholine	218:261	the major membrane lipid phosphatidylcholine (PC)	218:266	BACKGROUND & AIMS Patients with ulcerative colitis have low concentrations of the major membrane lipid phosphatidylcholine (PC) in gastrointestinal mucus, suggesting that defects in colonic PC metabolism might be involved in the development of colitis.
33238221	1	119	theme	lipid	237:241	arg1	PC					264:265	PC	264:265	PC	264:265	BACKGROUND & AIMS Patients with ulcerative colitis have low concentrations of the major membrane lipid phosphatidylcholine (PC) in gastrointestinal mucus, suggesting that defects in colonic PC metabolism might be involved in the development of colitis.
33238221	5	120	theme	isothiocyanate-dextran	1042:1063	arg1	gavage					1065:1070	fluorescein isothiocyanate-dextran gavage	1030:1070	fluorescein isothiocyanate-dextran gavage	1030:1070	Intestinal permeability was assessed by fluorescein isothiocyanate-dextran gavage and fecal microbial composition was analyzed by sequencing 16s ribosomal RNA amplicons.
33238221	6	121	theme	CTαIKO	1171:1176	arg1	mice					1178:1181	CTαIKO mice	1171:1181	CTαIKO mice	1171:1181	Subsets of CTαIKO mice and control mice were treated with dietary PC supplementation, antibiotics, or 4-phenylbutyrate.
33238221	3	122	theme	electron	783:790	arg1	microscopy					792:801	electron microscopy	783:801	electron microscopy	783:801	METHODS Colonic tissue of CTαIKO mice and control mice was analyzed by histology, immunofluorescence, electron microscopy, quantitative polymerase chain reaction, Western blot, and thin-layer chromatography.
33274927	4	0	theme	interpenetrating	615:630	arg1	technique					632:640	an original sequentially interpenetrating technique	590:640	an original sequentially interpenetrating technique based on the combination of a fast "click chemistry" and a slow enzymatic mediated cross-linking	590:737	To enhance recovery and widen applicability, the hypoxia-induced system was incorporated with a conductive network by an original sequentially interpenetrating technique based on the combination of a fast "click chemistry" and a slow enzymatic mediated cross-linking.
33274927	4	1	theme	"	693:693	arg1	combination					655:665	the combination	651:665	the combination of a fast "click chemistry" and a slow enzymatic mediated cross-linking	651:737	To enhance recovery and widen applicability, the hypoxia-induced system was incorporated with a conductive network by an original sequentially interpenetrating technique based on the combination of a fast "click chemistry" and a slow enzymatic mediated cross-linking.
33274927	6	2	theme	stem	1326:1329	arg1	cells					1331:1335	the encapsulated adipose-derived stem cells	1293:1335	the encapsulated adipose-derived stem cells	1293:1335	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	6	3	theme	hypoxia-inducing	1188:1203	arg1	capability					1205:1214	sustainable hypoxia-inducing capability	1176:1214	sustainable hypoxia-inducing capability	1176:1214	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	5	4	with	gelatin	977:983	arg1	rate					1040:1043	a slow cross-linking rate	1019:1043	a slow cross-linking rate	1019:1043	Hyperbranched poly(β-amino ester)-tetraaniline (PBAE-TA) was cross-linked with thiolated hyaluronic acid (HA-SH) via a thiol-ene click reaction, contributing to the rapid formation of the first conductive network, where vanillin-grafted gelatin (Geln-Van) and laccase (Lac) with a slow cross-linking rate were employed in casting a hypoxic microenvironment.
33274927	6	5	theme	encapsulated	1297:1308	arg1	cells					1331:1335	the encapsulated adipose-derived stem cells	1293:1335	the encapsulated adipose-derived stem cells	1293:1335	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	7	6	theme	clinical	1855:1862	arg1	symptoms					1864:1871	other complex clinical symptoms	1841:1871	other complex clinical symptoms	1841:1871	This work provides a promising strategy to broaden the applicability of diverse hydrogels with a long time-consuming gelation process and to integrate different networks with various biological functions for the therapies of diabetic wounds and other complex clinical symptoms.
33274927	4	7	theme	fast	672:675	arg1	"					693:693	a fast "click chemistry"	670:693	a fast "click chemistry"	670:693	To enhance recovery and widen applicability, the hypoxia-induced system was incorporated with a conductive network by an original sequentially interpenetrating technique based on the combination of a fast "click chemistry" and a slow enzymatic mediated cross-linking.
33274927	5	8	theme	Hyperbranched	740:752	arg1	PBAE-TA					788:794	PBAE-TA	788:794	PBAE-TA	788:794	Hyperbranched poly(β-amino ester)-tetraaniline (PBAE-TA) was cross-linked with thiolated hyaluronic acid (HA-SH) via a thiol-ene click reaction, contributing to the rapid formation of the first conductive network, where vanillin-grafted gelatin (Geln-Van) and laccase (Lac) with a slow cross-linking rate were employed in casting a hypoxic microenvironment.
33274927	5	8	theme	Hyperbranched	740:752	arg1	-tetraaniline					773:785	Hyperbranched poly(β-amino ester)-tetraaniline	740:785	Hyperbranched poly(β-amino ester)-tetraaniline (PBAE-TA)	740:795	Hyperbranched poly(β-amino ester)-tetraaniline (PBAE-TA) was cross-linked with thiolated hyaluronic acid (HA-SH) via a thiol-ene click reaction, contributing to the rapid formation of the first conductive network, where vanillin-grafted gelatin (Geln-Van) and laccase (Lac) with a slow cross-linking rate were employed in casting a hypoxic microenvironment.
33274927	6	9	theme	skin	1580:1583	arg1	functions					1585:1593	skin functions	1580:1593	skin functions	1580:1593	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	1	10	theme	tissue	216:221	arg1	regeneration					223:234	tissue regeneration	216:234	tissue regeneration	216:234	Injectable hydrogels with the capability to cast a hypoxic microenvironment is of great potentialities to develop novel therapies for tissue regeneration.
33274927	7	11	theme	gelation	1713:1720	arg1	process					1722:1728	a long time-consuming gelation process	1691:1728	a long time-consuming gelation process	1691:1728	This work provides a promising strategy to broaden the applicability of diverse hydrogels with a long time-consuming gelation process and to integrate different networks with various biological functions for the therapies of diabetic wounds and other complex clinical symptoms.
33274927	6	12	contain	possessed	1135:1143	arg2	conductivity					1159:1170	suitable conductivity	1150:1170	suitable conductivity	1150:1170	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	6	12	contain	possessed	1135:1143	arg2	capability					1205:1214	sustainable hypoxia-inducing capability	1176:1214	sustainable hypoxia-inducing capability	1176:1214	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	6	12	contain	possessed	1135:1143	arg1	hydrogels					1125:1133	The as-prepared injectable hydrogels	1098:1133	The as-prepared injectable hydrogels	1098:1133	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	4	13	theme	click	678:682	arg1	"					693:693	a fast "click chemistry"	670:693	a fast "click chemistry"	670:693	To enhance recovery and widen applicability, the hypoxia-induced system was incorporated with a conductive network by an original sequentially interpenetrating technique based on the combination of a fast "click chemistry" and a slow enzymatic mediated cross-linking.
33274927	5	14	theme	hypoxic	1072:1078	arg1	microenvironment					1080:1095	a hypoxic microenvironment	1070:1095	a hypoxic microenvironment	1070:1095	Hyperbranched poly(β-amino ester)-tetraaniline (PBAE-TA) was cross-linked with thiolated hyaluronic acid (HA-SH) via a thiol-ene click reaction, contributing to the rapid formation of the first conductive network, where vanillin-grafted gelatin (Geln-Van) and laccase (Lac) with a slow cross-linking rate were employed in casting a hypoxic microenvironment.
33274927	5	15	theme	click	869:873	arg1	reaction					875:882	a thiol-ene click reaction	857:882	a thiol-ene click reaction	857:882	Hyperbranched poly(β-amino ester)-tetraaniline (PBAE-TA) was cross-linked with thiolated hyaluronic acid (HA-SH) via a thiol-ene click reaction, contributing to the rapid formation of the first conductive network, where vanillin-grafted gelatin (Geln-Van) and laccase (Lac) with a slow cross-linking rate were employed in casting a hypoxic microenvironment.
33274927	7	16	theme	long	1693:1696	arg1	process					1722:1728	a long time-consuming gelation process	1691:1728	a long time-consuming gelation process	1691:1728	This work provides a promising strategy to broaden the applicability of diverse hydrogels with a long time-consuming gelation process and to integrate different networks with various biological functions for the therapies of diabetic wounds and other complex clinical symptoms.
33274927	7	17	with	networks	1757:1764	arg1	functions					1790:1798	various biological functions	1771:1798	various biological functions for the therapies of diabetic wounds and other complex clinical symptoms	1771:1871	This work provides a promising strategy to broaden the applicability of diverse hydrogels with a long time-consuming gelation process and to integrate different networks with various biological functions for the therapies of diabetic wounds and other complex clinical symptoms.
33274927	5	18	theme	first	928:932	arg1	network					945:951	the first conductive network	924:951	the first conductive network	924:951	Hyperbranched poly(β-amino ester)-tetraaniline (PBAE-TA) was cross-linked with thiolated hyaluronic acid (HA-SH) via a thiol-ene click reaction, contributing to the rapid formation of the first conductive network, where vanillin-grafted gelatin (Geln-Van) and laccase (Lac) with a slow cross-linking rate were employed in casting a hypoxic microenvironment.
33274927	6	19	theme	connexin	1266:1273	arg1	expressions					1278:1288	connexin 43 expressions	1266:1288	connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions	1266:1593	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	5	20	theme	network	945:951	arg1	formation					911:919	the rapid formation	901:919	the rapid formation	901:919	Hyperbranched poly(β-amino ester)-tetraaniline (PBAE-TA) was cross-linked with thiolated hyaluronic acid (HA-SH) via a thiol-ene click reaction, contributing to the rapid formation of the first conductive network, where vanillin-grafted gelatin (Geln-Van) and laccase (Lac) with a slow cross-linking rate were employed in casting a hypoxic microenvironment.
33274927	7	21	theme	diabetic	1821:1828	arg1	wounds					1830:1835	diabetic wounds	1821:1835	diabetic wounds	1821:1835	This work provides a promising strategy to broaden the applicability of diverse hydrogels with a long time-consuming gelation process and to integrate different networks with various biological functions for the therapies of diabetic wounds and other complex clinical symptoms.
33274927	7	22	theme	diverse	1668:1674	arg1	hydrogels					1676:1684	diverse hydrogels	1668:1684	diverse hydrogels with a long time-consuming gelation process	1668:1728	This work provides a promising strategy to broaden the applicability of diverse hydrogels with a long time-consuming gelation process and to integrate different networks with various biological functions for the therapies of diabetic wounds and other complex clinical symptoms.
33274927	4	23	theme	enzymatic	706:714	arg1	cross-linking					725:737	a slow enzymatic mediated cross-linking	699:737	a slow enzymatic mediated cross-linking	699:737	To enhance recovery and widen applicability, the hypoxia-induced system was incorporated with a conductive network by an original sequentially interpenetrating technique based on the combination of a fast "click chemistry" and a slow enzymatic mediated cross-linking.
33274927	5	24	theme	rapid	905:909	arg1	formation					911:919	the rapid formation	901:919	the rapid formation	901:919	Hyperbranched poly(β-amino ester)-tetraaniline (PBAE-TA) was cross-linked with thiolated hyaluronic acid (HA-SH) via a thiol-ene click reaction, contributing to the rapid formation of the first conductive network, where vanillin-grafted gelatin (Geln-Van) and laccase (Lac) with a slow cross-linking rate were employed in casting a hypoxic microenvironment.
33274927	3	25	theme	diabetic	324:331	arg1	model					366:370	a representative injury model	342:370	a representative injury model to explore the actual therapeutic results of tissue injury by injectable hypoxia-induced hydrogels	342:469	Herein, we chose diabetic wound as a representative injury model to explore the actual therapeutic results of tissue injury by injectable hypoxia-induced hydrogels.
33274927	3	25	theme	diabetic	324:331	arg1	wound					333:337	diabetic wound	324:337	diabetic wound	324:337	Herein, we chose diabetic wound as a representative injury model to explore the actual therapeutic results of tissue injury by injectable hypoxia-induced hydrogels.
33274927	4	26	theme	cross-linking	725:737	arg1	combination					655:665	the combination	651:665	the combination of a fast "click chemistry" and a slow enzymatic mediated cross-linking	651:737	To enhance recovery and widen applicability, the hypoxia-induced system was incorporated with a conductive network by an original sequentially interpenetrating technique based on the combination of a fast "click chemistry" and a slow enzymatic mediated cross-linking.
33274927	6	27	theme	hair	1454:1457	arg1	follicles					1459:1467	hair follicles	1454:1467	hair follicles	1454:1467	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	7	28	with	hydrogels	1676:1684	arg1	process					1722:1728	a long time-consuming gelation process	1691:1728	a long time-consuming gelation process	1691:1728	This work provides a promising strategy to broaden the applicability of diverse hydrogels with a long time-consuming gelation process and to integrate different networks with various biological functions for the therapies of diabetic wounds and other complex clinical symptoms.
33274927	6	29	theme	vessels	1445:1451	arg1	reconstruction					1421:1434	the reconstruction	1417:1434	the reconstruction of blood vessels, hair follicles, and dermal collagen matrix	1417:1495	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	7	30	theme	biological	1779:1788	arg1	functions					1790:1798	various biological functions	1771:1798	various biological functions for the therapies of diabetic wounds and other complex clinical symptoms	1771:1871	This work provides a promising strategy to broaden the applicability of diverse hydrogels with a long time-consuming gelation process and to integrate different networks with various biological functions for the therapies of diabetic wounds and other complex clinical symptoms.
33274927	3	31	theme	representative	344:357	arg1	model					366:370	a representative injury model	342:370	a representative injury model to explore the actual therapeutic results of tissue injury by injectable hypoxia-induced hydrogels	342:469	Herein, we chose diabetic wound as a representative injury model to explore the actual therapeutic results of tissue injury by injectable hypoxia-induced hydrogels.
33274927	3	31	theme	representative	344:357	arg1	wound					333:337	diabetic wound	324:337	diabetic wound	324:337	Herein, we chose diabetic wound as a representative injury model to explore the actual therapeutic results of tissue injury by injectable hypoxia-induced hydrogels.
33274927	3	32	theme	hypoxia-induced	445:459	arg1	hydrogels					461:469	injectable hypoxia-induced hydrogels	434:469	injectable hypoxia-induced hydrogels	434:469	Herein, we chose diabetic wound as a representative injury model to explore the actual therapeutic results of tissue injury by injectable hypoxia-induced hydrogels.
33274927	3	33	theme	actual	387:392	arg1	results					406:412	the actual therapeutic results	383:412	the actual therapeutic results of tissue injury	383:429	Herein, we chose diabetic wound as a representative injury model to explore the actual therapeutic results of tissue injury by injectable hypoxia-induced hydrogels.
33274927	0	34	theme	Injectable	0:9	arg1	Hydrogel					38:45	Injectable Hypoxia-Induced Conductive Hydrogel	0:45	Injectable Hypoxia-Induced Conductive Hydrogel	0:45	Injectable Hypoxia-Induced Conductive Hydrogel to Promote Diabetic Wound Healing.
33274927	7	35	theme	different	1747:1755	arg1	networks					1757:1764	different networks	1747:1764	different networks with various biological functions for the therapies of diabetic wounds and other complex clinical symptoms	1747:1871	This work provides a promising strategy to broaden the applicability of diverse hydrogels with a long time-consuming gelation process and to integrate different networks with various biological functions for the therapies of diabetic wounds and other complex clinical symptoms.
33274927	5	36	theme	thiolated	819:827	arg1	HA-SH					846:850	HA-SH	846:850	HA-SH	846:850	Hyperbranched poly(β-amino ester)-tetraaniline (PBAE-TA) was cross-linked with thiolated hyaluronic acid (HA-SH) via a thiol-ene click reaction, contributing to the rapid formation of the first conductive network, where vanillin-grafted gelatin (Geln-Van) and laccase (Lac) with a slow cross-linking rate were employed in casting a hypoxic microenvironment.
33274927	5	36	theme	thiolated	819:827	arg1	acid					840:843	thiolated hyaluronic acid	819:843	thiolated hyaluronic acid (HA-SH) via a thiol-ene click reaction	819:882	Hyperbranched poly(β-amino ester)-tetraaniline (PBAE-TA) was cross-linked with thiolated hyaluronic acid (HA-SH) via a thiol-ene click reaction, contributing to the rapid formation of the first conductive network, where vanillin-grafted gelatin (Geln-Van) and laccase (Lac) with a slow cross-linking rate were employed in casting a hypoxic microenvironment.
33274927	0	37	theme	Conductive	27:36	arg1	Hydrogel					38:45	Injectable Hypoxia-Induced Conductive Hydrogel	0:45	Injectable Hypoxia-Induced Conductive Hydrogel	0:45	Injectable Hypoxia-Induced Conductive Hydrogel to Promote Diabetic Wound Healing.
33274927	3	38	theme	tissue	417:422	arg1	injury					424:429	tissue injury	417:429	tissue injury	417:429	Herein, we chose diabetic wound as a representative injury model to explore the actual therapeutic results of tissue injury by injectable hypoxia-induced hydrogels.
33274927	6	39	theme	matrix	1490:1495	arg1	reconstruction					1421:1434	the reconstruction	1417:1434	the reconstruction of blood vessels, hair follicles, and dermal collagen matrix	1417:1495	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	6	40	theme	dermal	1474:1479	arg1	matrix					1490:1495	dermal collagen matrix	1474:1495	dermal collagen matrix	1474:1495	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	5	41	theme	cross-linking	1026:1038	arg1	rate					1040:1043	a slow cross-linking rate	1019:1043	a slow cross-linking rate	1019:1043	Hyperbranched poly(β-amino ester)-tetraaniline (PBAE-TA) was cross-linked with thiolated hyaluronic acid (HA-SH) via a thiol-ene click reaction, contributing to the rapid formation of the first conductive network, where vanillin-grafted gelatin (Geln-Van) and laccase (Lac) with a slow cross-linking rate were employed in casting a hypoxic microenvironment.
33274927	6	42	theme	skin	1549:1552	arg1	wounds					1554:1559	diabetic rat skin wounds	1536:1559	diabetic rat skin wounds	1536:1559	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	0	43	theme	Wound	67:71	arg1	Healing					73:79	Diabetic Wound Healing	58:79	Diabetic Wound Healing	58:79	Injectable Hypoxia-Induced Conductive Hydrogel to Promote Diabetic Wound Healing.
33274927	6	44	theme	as-prepared	1102:1112	arg1	hydrogels					1125:1133	The as-prepared injectable hydrogels	1098:1133	The as-prepared injectable hydrogels	1098:1133	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	5	45	with	laccase	1000:1006	arg1	rate					1040:1043	a slow cross-linking rate	1019:1043	a slow cross-linking rate	1019:1043	Hyperbranched poly(β-amino ester)-tetraaniline (PBAE-TA) was cross-linked with thiolated hyaluronic acid (HA-SH) via a thiol-ene click reaction, contributing to the rapid formation of the first conductive network, where vanillin-grafted gelatin (Geln-Van) and laccase (Lac) with a slow cross-linking rate were employed in casting a hypoxic microenvironment.
33274927	6	46	theme	vascular	1353:1360	arg1	regeneration					1362:1373	vascular regeneration	1353:1373	vascular regeneration	1353:1373	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	6	47	theme	diabetic	1536:1543	arg1	wounds					1554:1559	diabetic rat skin wounds	1536:1559	diabetic rat skin wounds	1536:1559	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	6	48	theme	cells	1331:1335	arg1	expressions					1278:1288	connexin 43 expressions	1266:1288	connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions	1266:1593	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	6	48	theme	cells	1331:1335	arg1	factor-1α					1252:1260	the hypoxia-inducible factor-1α	1230:1260	the hypoxia-inducible factor-1α	1230:1260	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	2	49	theme	conceptual	289:298	arg1	phase					300:304	the conceptual phase	285:304	the conceptual phase	285:304	However, the relative research still remains at the conceptual phase.
33274927	5	50	theme	hyaluronic	829:838	arg1	HA-SH					846:850	HA-SH	846:850	HA-SH	846:850	Hyperbranched poly(β-amino ester)-tetraaniline (PBAE-TA) was cross-linked with thiolated hyaluronic acid (HA-SH) via a thiol-ene click reaction, contributing to the rapid formation of the first conductive network, where vanillin-grafted gelatin (Geln-Van) and laccase (Lac) with a slow cross-linking rate were employed in casting a hypoxic microenvironment.
33274927	5	50	theme	hyaluronic	829:838	arg1	acid					840:843	thiolated hyaluronic acid	819:843	thiolated hyaluronic acid (HA-SH) via a thiol-ene click reaction	819:882	Hyperbranched poly(β-amino ester)-tetraaniline (PBAE-TA) was cross-linked with thiolated hyaluronic acid (HA-SH) via a thiol-ene click reaction, contributing to the rapid formation of the first conductive network, where vanillin-grafted gelatin (Geln-Van) and laccase (Lac) with a slow cross-linking rate were employed in casting a hypoxic microenvironment.
33274927	7	51	theme	symptoms	1864:1871	arg1	therapies					1808:1816	the therapies	1804:1816	the therapies of diabetic wounds and other complex clinical symptoms	1804:1871	This work provides a promising strategy to broaden the applicability of diverse hydrogels with a long time-consuming gelation process and to integrate different networks with various biological functions for the therapies of diabetic wounds and other complex clinical symptoms.
33274927	4	52	theme	chemistry	684:692	arg1	"					693:693	a fast "click chemistry"	670:693	a fast "click chemistry"	670:693	To enhance recovery and widen applicability, the hypoxia-induced system was incorporated with a conductive network by an original sequentially interpenetrating technique based on the combination of a fast "click chemistry" and a slow enzymatic mediated cross-linking.
33274927	6	53	theme	adipose-derived	1310:1324	arg1	cells					1331:1335	the encapsulated adipose-derived stem cells	1293:1335	the encapsulated adipose-derived stem cells	1293:1335	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	1	54	theme	novel	196:200	arg1	therapies					202:210	novel therapies	196:210	novel therapies for tissue regeneration	196:234	Injectable hydrogels with the capability to cast a hypoxic microenvironment is of great potentialities to develop novel therapies for tissue regeneration.
33274927	7	55	theme	complex	1847:1853	arg1	symptoms					1864:1871	other complex clinical symptoms	1841:1871	other complex clinical symptoms	1841:1871	This work provides a promising strategy to broaden the applicability of diverse hydrogels with a long time-consuming gelation process and to integrate different networks with various biological functions for the therapies of diabetic wounds and other complex clinical symptoms.
33274927	6	56	theme	sustainable	1176:1186	arg1	capability					1205:1214	sustainable hypoxia-inducing capability	1176:1214	sustainable hypoxia-inducing capability	1176:1214	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	6	57	link	adipose-derived	1310:1324	arg1	cells					1331:1335	the encapsulated adipose-derived stem cells	1293:1335	the encapsulated adipose-derived stem cells	1293:1335	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	6	58	theme	functions	1585:1593	arg1	recovery					1524:1531	the recovery	1520:1531	the recovery of diabetic rat skin wounds	1520:1559	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	6	58	theme	functions	1585:1593	arg1	restoration					1565:1575	restoration	1565:1575	restoration of skin functions	1565:1593	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	7	59	theme	time-consuming	1698:1711	arg1	process					1722:1728	a long time-consuming gelation process	1691:1728	a long time-consuming gelation process	1691:1728	This work provides a promising strategy to broaden the applicability of diverse hydrogels with a long time-consuming gelation process and to integrate different networks with various biological functions for the therapies of diabetic wounds and other complex clinical symptoms.
33274927	5	60	theme	poly	754:757	arg1	PBAE-TA					788:794	PBAE-TA	788:794	PBAE-TA	788:794	Hyperbranched poly(β-amino ester)-tetraaniline (PBAE-TA) was cross-linked with thiolated hyaluronic acid (HA-SH) via a thiol-ene click reaction, contributing to the rapid formation of the first conductive network, where vanillin-grafted gelatin (Geln-Van) and laccase (Lac) with a slow cross-linking rate were employed in casting a hypoxic microenvironment.
33274927	5	60	theme	poly	754:757	arg1	-tetraaniline					773:785	Hyperbranched poly(β-amino ester)-tetraaniline	740:785	Hyperbranched poly(β-amino ester)-tetraaniline (PBAE-TA)	740:795	Hyperbranched poly(β-amino ester)-tetraaniline (PBAE-TA) was cross-linked with thiolated hyaluronic acid (HA-SH) via a thiol-ene click reaction, contributing to the rapid formation of the first conductive network, where vanillin-grafted gelatin (Geln-Van) and laccase (Lac) with a slow cross-linking rate were employed in casting a hypoxic microenvironment.
33274927	2	61	theme	relative	250:257	arg1	research					259:266	the relative research	246:266	the relative research	246:266	However, the relative research still remains at the conceptual phase.
33274927	5	62	theme	thiol-ene	859:867	arg1	reaction					875:882	a thiol-ene click reaction	857:882	a thiol-ene click reaction	857:882	Hyperbranched poly(β-amino ester)-tetraaniline (PBAE-TA) was cross-linked with thiolated hyaluronic acid (HA-SH) via a thiol-ene click reaction, contributing to the rapid formation of the first conductive network, where vanillin-grafted gelatin (Geln-Van) and laccase (Lac) with a slow cross-linking rate were employed in casting a hypoxic microenvironment.
33274927	1	63	with	hydrogels	93:101	arg1	capability					112:121	the capability to cast a hypoxic microenvironment	108:156	the capability to cast a hypoxic microenvironment	108:156	Injectable hydrogels with the capability to cast a hypoxic microenvironment is of great potentialities to develop novel therapies for tissue regeneration.
33274927	7	64	theme	hydrogels	1676:1684	arg1	applicability					1651:1663	the applicability	1647:1663	the applicability of diverse hydrogels with a long time-consuming gelation process	1647:1728	This work provides a promising strategy to broaden the applicability of diverse hydrogels with a long time-consuming gelation process and to integrate different networks with various biological functions for the therapies of diabetic wounds and other complex clinical symptoms.
33274927	7	65	dep	strategy	1627:1634	arg1	broaden					1639:1645	broaden	1639:1645	to broaden the applicability of diverse hydrogels with a long time-consuming gelation process	1636:1728	This work provides a promising strategy to broaden the applicability of diverse hydrogels with a long time-consuming gelation process and to integrate different networks with various biological functions for the therapies of diabetic wounds and other complex clinical symptoms.
33274927	7	65	dep	strategy	1627:1634	arg1	integrate					1737:1745	integrate	1737:1745	to integrate different networks with various biological functions for the therapies of diabetic wounds and other complex clinical symptoms	1734:1871	This work provides a promising strategy to broaden the applicability of diverse hydrogels with a long time-consuming gelation process and to integrate different networks with various biological functions for the therapies of diabetic wounds and other complex clinical symptoms.
33274927	1	66	theme	hypoxic	133:139	arg1	microenvironment					141:156	a hypoxic microenvironment	131:156	a hypoxic microenvironment	131:156	Injectable hydrogels with the capability to cast a hypoxic microenvironment is of great potentialities to develop novel therapies for tissue regeneration.
33274927	7	67	theme	other	1841:1845	arg1	symptoms					1864:1871	other complex clinical symptoms	1841:1871	other complex clinical symptoms	1841:1871	This work provides a promising strategy to broaden the applicability of diverse hydrogels with a long time-consuming gelation process and to integrate different networks with various biological functions for the therapies of diabetic wounds and other complex clinical symptoms.
33274927	7	68	theme	wounds	1830:1835	arg1	therapies					1808:1816	the therapies	1804:1816	the therapies of diabetic wounds and other complex clinical symptoms	1804:1871	This work provides a promising strategy to broaden the applicability of diverse hydrogels with a long time-consuming gelation process and to integrate different networks with various biological functions for the therapies of diabetic wounds and other complex clinical symptoms.
33274927	4	69	theme	hypoxia-induced	521:535	arg1	system					537:542	the hypoxia-induced system	517:542	the hypoxia-induced system	517:542	To enhance recovery and widen applicability, the hypoxia-induced system was incorporated with a conductive network by an original sequentially interpenetrating technique based on the combination of a fast "click chemistry" and a slow enzymatic mediated cross-linking.
33274927	6	70	theme	hypoxia-inducible	1234:1250	arg1	factor-1α					1252:1260	the hypoxia-inducible factor-1α	1230:1260	the hypoxia-inducible factor-1α	1230:1260	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	4	71	theme	slow	701:704	arg1	cross-linking					725:737	a slow enzymatic mediated cross-linking	699:737	a slow enzymatic mediated cross-linking	699:737	To enhance recovery and widen applicability, the hypoxia-induced system was incorporated with a conductive network by an original sequentially interpenetrating technique based on the combination of a fast "click chemistry" and a slow enzymatic mediated cross-linking.
33274927	6	72	theme	follicles	1459:1467	arg1	reconstruction					1421:1434	the reconstruction	1417:1434	the reconstruction of blood vessels, hair follicles, and dermal collagen matrix	1417:1495	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	1	73	theme	great	164:168	arg1	potentialities					170:183	great potentialities	164:183	great potentialities	164:183	Injectable hydrogels with the capability to cast a hypoxic microenvironment is of great potentialities to develop novel therapies for tissue regeneration.
33274927	4	74	theme	mediated	716:723	arg1	cross-linking					725:737	a slow enzymatic mediated cross-linking	699:737	a slow enzymatic mediated cross-linking	699:737	To enhance recovery and widen applicability, the hypoxia-induced system was incorporated with a conductive network by an original sequentially interpenetrating technique based on the combination of a fast "click chemistry" and a slow enzymatic mediated cross-linking.
33274927	5	75	theme	β-amino	759:765	arg1	poly					754:757	poly	754:757	Hyperbranched poly(β-amino ester)-tetraaniline (PBAE-TA)	740:795	Hyperbranched poly(β-amino ester)-tetraaniline (PBAE-TA) was cross-linked with thiolated hyaluronic acid (HA-SH) via a thiol-ene click reaction, contributing to the rapid formation of the first conductive network, where vanillin-grafted gelatin (Geln-Van) and laccase (Lac) with a slow cross-linking rate were employed in casting a hypoxic microenvironment.
33274927	5	75	theme	β-amino	759:765	arg1	ester					767:771	β-amino ester	759:771	β-amino ester	759:771	Hyperbranched poly(β-amino ester)-tetraaniline (PBAE-TA) was cross-linked with thiolated hyaluronic acid (HA-SH) via a thiol-ene click reaction, contributing to the rapid formation of the first conductive network, where vanillin-grafted gelatin (Geln-Van) and laccase (Lac) with a slow cross-linking rate were employed in casting a hypoxic microenvironment.
33274927	7	76	theme	promising	1617:1625	arg1	strategy					1627:1634	a promising strategy	1615:1634	a promising strategy to broaden the applicability of diverse hydrogels with a long time-consuming gelation process and to integrate different networks with various biological functions for the therapies of diabetic wounds and other complex clinical symptoms	1615:1871	This work provides a promising strategy to broaden the applicability of diverse hydrogels with a long time-consuming gelation process and to integrate different networks with various biological functions for the therapies of diabetic wounds and other complex clinical symptoms.
33274927	6	77	theme	blood	1439:1443	arg1	vessels					1445:1451	blood vessels	1439:1451	blood vessels	1439:1451	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	1	78	theme	Injectable	82:91	arg1	hydrogels					93:101	Injectable hydrogels	82:101	Injectable hydrogels with the capability to cast a hypoxic microenvironment	82:156	Injectable hydrogels with the capability to cast a hypoxic microenvironment is of great potentialities to develop novel therapies for tissue regeneration.
33274927	5	79	theme	conductive	934:943	arg1	network					945:951	the first conductive network	924:951	the first conductive network	924:951	Hyperbranched poly(β-amino ester)-tetraaniline (PBAE-TA) was cross-linked with thiolated hyaluronic acid (HA-SH) via a thiol-ene click reaction, contributing to the rapid formation of the first conductive network, where vanillin-grafted gelatin (Geln-Van) and laccase (Lac) with a slow cross-linking rate were employed in casting a hypoxic microenvironment.
33274927	3	80	theme	injury	359:364	arg1	model					366:370	a representative injury model	342:370	a representative injury model to explore the actual therapeutic results of tissue injury by injectable hypoxia-induced hydrogels	342:469	Herein, we chose diabetic wound as a representative injury model to explore the actual therapeutic results of tissue injury by injectable hypoxia-induced hydrogels.
33274927	3	80	theme	injury	359:364	arg1	wound					333:337	diabetic wound	324:337	diabetic wound	324:337	Herein, we chose diabetic wound as a representative injury model to explore the actual therapeutic results of tissue injury by injectable hypoxia-induced hydrogels.
33274927	0	81	theme	Hypoxia-Induced	11:25	arg1	Hydrogel					38:45	Injectable Hypoxia-Induced Conductive Hydrogel	0:45	Injectable Hypoxia-Induced Conductive Hydrogel	0:45	Injectable Hypoxia-Induced Conductive Hydrogel to Promote Diabetic Wound Healing.
33274927	7	82	theme	various	1771:1777	arg1	functions					1790:1798	various biological functions	1771:1798	various biological functions for the therapies of diabetic wounds and other complex clinical symptoms	1771:1871	This work provides a promising strategy to broaden the applicability of diverse hydrogels with a long time-consuming gelation process and to integrate different networks with various biological functions for the therapies of diabetic wounds and other complex clinical symptoms.
33274927	3	83	theme	therapeutic	394:404	arg1	results					406:412	the actual therapeutic results	383:412	the actual therapeutic results of tissue injury	383:429	Herein, we chose diabetic wound as a representative injury model to explore the actual therapeutic results of tissue injury by injectable hypoxia-induced hydrogels.
33274927	3	84	theme	injectable	434:443	arg1	hydrogels					461:469	injectable hypoxia-induced hydrogels	434:469	injectable hypoxia-induced hydrogels	434:469	Herein, we chose diabetic wound as a representative injury model to explore the actual therapeutic results of tissue injury by injectable hypoxia-induced hydrogels.
33274927	5	85	theme	vanillin-grafted	960:975	arg1	Geln-Van					986:993	Geln-Van	986:993	Geln-Van	986:993	Hyperbranched poly(β-amino ester)-tetraaniline (PBAE-TA) was cross-linked with thiolated hyaluronic acid (HA-SH) via a thiol-ene click reaction, contributing to the rapid formation of the first conductive network, where vanillin-grafted gelatin (Geln-Van) and laccase (Lac) with a slow cross-linking rate were employed in casting a hypoxic microenvironment.
33274927	5	85	theme	vanillin-grafted	960:975	arg1	gelatin					977:983	vanillin-grafted gelatin	960:983	vanillin-grafted gelatin (Geln-Van)	960:994	Hyperbranched poly(β-amino ester)-tetraaniline (PBAE-TA) was cross-linked with thiolated hyaluronic acid (HA-SH) via a thiol-ene click reaction, contributing to the rapid formation of the first conductive network, where vanillin-grafted gelatin (Geln-Van) and laccase (Lac) with a slow cross-linking rate were employed in casting a hypoxic microenvironment.
33274927	4	86	theme	original	593:600	arg1	technique					632:640	an original sequentially interpenetrating technique	590:640	an original sequentially interpenetrating technique based on the combination of a fast "click chemistry" and a slow enzymatic mediated cross-linking	590:737	To enhance recovery and widen applicability, the hypoxia-induced system was incorporated with a conductive network by an original sequentially interpenetrating technique based on the combination of a fast "click chemistry" and a slow enzymatic mediated cross-linking.
33274927	0	87	theme	Diabetic	58:65	arg1	Healing					73:79	Diabetic Wound Healing	58:79	Diabetic Wound Healing	58:79	Injectable Hypoxia-Induced Conductive Hydrogel to Promote Diabetic Wound Healing.
33274927	3	88	theme	injury	424:429	arg1	results					406:412	the actual therapeutic results	383:412	the actual therapeutic results of tissue injury	383:429	Herein, we chose diabetic wound as a representative injury model to explore the actual therapeutic results of tissue injury by injectable hypoxia-induced hydrogels.
33274927	6	89	theme	collagen	1481:1488	arg1	matrix					1490:1495	dermal collagen matrix	1474:1495	dermal collagen matrix	1474:1495	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	5	90	theme	slow	1021:1024	arg1	rate					1040:1043	a slow cross-linking rate	1019:1043	a slow cross-linking rate	1019:1043	Hyperbranched poly(β-amino ester)-tetraaniline (PBAE-TA) was cross-linked with thiolated hyaluronic acid (HA-SH) via a thiol-ene click reaction, contributing to the rapid formation of the first conductive network, where vanillin-grafted gelatin (Geln-Van) and laccase (Lac) with a slow cross-linking rate were employed in casting a hypoxic microenvironment.
33274927	6	91	theme	suitable	1150:1157	arg1	conductivity					1159:1170	suitable conductivity	1150:1170	suitable conductivity	1150:1170	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	4	92	theme	conductive	568:577	arg1	network					579:585	a conductive network	566:585	a conductive network	566:585	To enhance recovery and widen applicability, the hypoxia-induced system was incorporated with a conductive network by an original sequentially interpenetrating technique based on the combination of a fast "click chemistry" and a slow enzymatic mediated cross-linking.
33274927	6	93	theme	wounds	1554:1559	arg1	recovery					1524:1531	the recovery	1520:1531	the recovery of diabetic rat skin wounds	1520:1559	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	6	93	theme	wounds	1554:1559	arg1	restoration					1565:1575	restoration	1565:1575	restoration of skin functions	1565:1593	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	6	94	theme	injectable	1114:1123	arg1	hydrogels					1125:1133	The as-prepared injectable hydrogels	1098:1133	The as-prepared injectable hydrogels	1098:1133	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
33274927	6	95	theme	rat	1545:1547	arg1	wounds					1554:1559	diabetic rat skin wounds	1536:1559	diabetic rat skin wounds	1536:1559	The as-prepared injectable hydrogels possessed both suitable conductivity and sustainable hypoxia-inducing capability to upregulate the hypoxia-inducible factor-1α and connexin 43 expressions of the encapsulated adipose-derived stem cells, which enhanced vascular regeneration and immunoregulation and further promoted the reconstruction of blood vessels, hair follicles, and dermal collagen matrix, eventually leading to the recovery of diabetic rat skin wounds and restoration of skin functions.
31955203	8	0	dep	RESULTS	1006:1012	arg1	correlated					1055:1064	correlated	1055:1064	correlated with serum and colonic cathelicidin	1055:1100	RESULTS In UC patients, serum 25(OH)D positively correlated with serum and colonic cathelicidin.
31955203	10	1	theme	cells	1302:1306	arg1	treatment					1283:1291	The 1,25(OH)2D treatment	1268:1291	The 1,25(OH)2D treatment of colon cells	1268:1306	The 1,25(OH)2D treatment of colon cells induced cathelicidin and IL-10, repressed TNF-α, and suppressed Escherichia coli growth.
31955203	3	2	theme	human	504:508	arg1	cathelicidin					510:521	intrarectal human cathelicidin	492:521	intrarectal human cathelicidin	492:521	Our goal was to evaluate the link between cathelicidin and vitamin D-associated clinical outcomes in UC patients, explore vitamin D induction of cathelicidin in human colon cells, and evaluate the effects of intrarectal human cathelicidin on a murine model of colitis.
31955203	6	3	theme	culture	849:855	arg1	supernatants					857:868	cell culture supernatants	844:868	cell culture supernatants	844:868	Antimicrobial activity against Escherichia coli from cell culture supernatants was measured.
31955203	4	4	theme	UC	616:617	arg1	patients					619:626	UC patients	616:626	UC patients	616:626	METHODS Serum and colonic cathelicidin levels were measured in UC patients and correlated with clinical and histologic outcomes.
31955203	10	5	dep	Escherichia	1372:1382	arg1	coli					1384:1387	coli	1384:1387	coli	1384:1387	The 1,25(OH)2D treatment of colon cells induced cathelicidin and IL-10, repressed TNF-α, and suppressed Escherichia coli growth.
31955203	1	6	theme	ulcerative	159:168	arg1	colitis					170:176	ulcerative colitis	159:176	ulcerative colitis (UC) patients	159:190	BACKGROUND Vitamin D plays a protective role in ulcerative colitis (UC) patients through unclear mechanisms.
31955203	1	6	theme	ulcerative	159:168	arg1	UC					179:180	UC	179:180	UC	179:180	BACKGROUND Vitamin D plays a protective role in ulcerative colitis (UC) patients through unclear mechanisms.
31955203	13	7	theme	murine	1704:1709	arg1	colitis					1711:1717	murine colitis	1704:1717	murine colitis	1704:1717	CONCLUSIONS Cathelicidin plays a protective role in 25(OH)D-associated UC histologic outcomes and murine colitis.
31955203	15	8	theme	vitamin	1881:1887	arg1	pathway					1904:1910	the vitamin D-cathelicidin pathway	1877:1910	the vitamin D-cathelicidin pathway	1877:1910	Our study provides insights into the vitamin D-cathelicidin pathway as a potential therapeutic target.
31955203	9	9	theme	histologic	1166:1175	arg1	inflammation					1177:1188	histologic inflammation	1166:1188	histologic inflammation	1166:1188	Higher serum cathelicidin is associated with decreased risk of histologic inflammation and clinical relapse but not independent of 25(OH)D or baseline inflammation.
31955203	3	10	theme	murine	528:533	arg1	model					535:539	a murine model	526:539	a murine model of colitis	526:550	Our goal was to evaluate the link between cathelicidin and vitamin D-associated clinical outcomes in UC patients, explore vitamin D induction of cathelicidin in human colon cells, and evaluate the effects of intrarectal human cathelicidin on a murine model of colitis.
31955203	13	11	theme	UC	1677:1678	arg1	outcomes					1691:1698	25(OH)D-associated UC histologic outcomes	1658:1698	25(OH)D-associated UC histologic outcomes	1658:1698	CONCLUSIONS Cathelicidin plays a protective role in 25(OH)D-associated UC histologic outcomes and murine colitis.
31955203	5	12	theme	Human	682:686	arg1	cells					694:698	Human colon cells	682:698	Human colon cells	682:698	Human colon cells were treated with 1,25(OH)2D and production of cathelicidin and cytokines were quantified.
31955203	9	13	theme	relapse	1203:1209	arg1	risk					1158:1161	decreased risk	1148:1161	decreased risk of histologic inflammation and clinical relapse	1148:1209	Higher serum cathelicidin is associated with decreased risk of histologic inflammation and clinical relapse but not independent of 25(OH)D or baseline inflammation.
31955203	15	14	dep	potential	1917:1925	arg1	therapeutic					1927:1937	therapeutic	1927:1937	therapeutic	1927:1937	Our study provides insights into the vitamin D-cathelicidin pathway as a potential therapeutic target.
31955203	12	15	theme	colitis	1529:1535	arg1	severity					1513:1520	the severity	1509:1520	the severity of DSS colitis	1509:1535	Intrarectal cathelicidin reduced the severity of DSS colitis but did not mitigate the impact of colitis on microbial composition.
31955203	1	16	theme	BACKGROUND	111:120	arg1	D					130:130	BACKGROUND Vitamin D	111:130	BACKGROUND Vitamin D	111:130	BACKGROUND Vitamin D plays a protective role in ulcerative colitis (UC) patients through unclear mechanisms.
31955203	2	17	theme	antimicrobial	239:251	arg1	Cathelicidin					220:231	Cathelicidin	220:231	Cathelicidin	220:231	Cathelicidin is an antimicrobial peptide induced by 1,25(OH)D2.
31955203	2	17	theme	antimicrobial	239:251	arg1	peptide					253:259	an antimicrobial peptide	236:259	an antimicrobial peptide induced by 1,25(OH)D2	236:281	Cathelicidin is an antimicrobial peptide induced by 1,25(OH)D2.
31955203	7	18	theme	DSS	952:954	arg1	colitis					956:962	DSS colitis	952:962	DSS colitis	952:962	Mice were treated with intrarectal cathelicidin, and its effects on DSS colitis and intestinal microbiota were evaluated.
31955203	14	19	theme	antimicrobial	1804:1816	arg1	activity					1818:1825	antimicrobial activity	1804:1825	antimicrobial activity against E. coli	1804:1841	Cathelicidin is induced by vitamin D in human colonic epithelial cells and promotes antimicrobial activity against E. coli.
31955203	11	20	theme	antimicrobial	1402:1414	arg1	effect					1416:1421	This antimicrobial effect	1397:1421	This antimicrobial effect	1397:1421	This antimicrobial effect was attenuated with siRNA-cathelicidin transfection.
31955203	13	21	theme	25	1658:1659	arg1	outcomes					1691:1698	25(OH)D-associated UC histologic outcomes	1658:1698	25(OH)D-associated UC histologic outcomes	1658:1698	CONCLUSIONS Cathelicidin plays a protective role in 25(OH)D-associated UC histologic outcomes and murine colitis.
31955203	14	22	theme	colonic	1766:1772	arg1	cells					1785:1789	human colonic epithelial cells	1760:1789	human colonic epithelial cells	1760:1789	Cathelicidin is induced by vitamin D in human colonic epithelial cells and promotes antimicrobial activity against E. coli.
31955203	0	23	theme	Human	79:83	arg1	Cells					104:108	Human Colonic Epithelial Cells	79:108	Human Colonic Epithelial Cells	79:108	Cathelicidin Mediates a Protective Role of Vitamin D in Ulcerative Colitis and Human Colonic Epithelial Cells.
31955203	3	24	from	cathelicidin	326:337	arg1	patients					388:395	UC patients	385:395	UC patients	385:395	Our goal was to evaluate the link between cathelicidin and vitamin D-associated clinical outcomes in UC patients, explore vitamin D induction of cathelicidin in human colon cells, and evaluate the effects of intrarectal human cathelicidin on a murine model of colitis.
31955203	2	25	theme	1,25	272:275	arg1	D2					280:281	1,25(OH)D2	272:281	1,25(OH)D2	272:281	Cathelicidin is an antimicrobial peptide induced by 1,25(OH)D2.
31955203	15	26	theme	D-cathelicidin	1889:1902	arg1	pathway					1904:1910	the vitamin D-cathelicidin pathway	1877:1910	the vitamin D-cathelicidin pathway	1877:1910	Our study provides insights into the vitamin D-cathelicidin pathway as a potential therapeutic target.
31955203	0	27	theme	Epithelial	93:102	arg1	Cells					104:108	Human Colonic Epithelial Cells	79:108	Human Colonic Epithelial Cells	79:108	Cathelicidin Mediates a Protective Role of Vitamin D in Ulcerative Colitis and Human Colonic Epithelial Cells.
31955203	9	28	theme	inflammation	1254:1265	arg1	independent					1219:1229	independent	1219:1229	independent	1219:1229	Higher serum cathelicidin is associated with decreased risk of histologic inflammation and clinical relapse but not independent of 25(OH)D or baseline inflammation.
31955203	8	29	theme	UC	1017:1018	arg1	patients					1020:1027	UC patients	1017:1027	UC patients	1017:1027	RESULTS In UC patients, serum 25(OH)D positively correlated with serum and colonic cathelicidin.
31955203	3	30	theme	vitamin	343:349	arg1	outcomes					373:380	vitamin D-associated clinical outcomes	343:380	vitamin D-associated clinical outcomes in UC patients	343:395	Our goal was to evaluate the link between cathelicidin and vitamin D-associated clinical outcomes in UC patients, explore vitamin D induction of cathelicidin in human colon cells, and evaluate the effects of intrarectal human cathelicidin on a murine model of colitis.
31955203	0	31	from	Role	35:38	arg1	Colitis					67:73	Ulcerative Colitis	56:73	Ulcerative Colitis	56:73	Cathelicidin Mediates a Protective Role of Vitamin D in Ulcerative Colitis and Human Colonic Epithelial Cells.
31955203	0	31	from	Role	35:38	arg1	Cells					104:108	Human Colonic Epithelial Cells	79:108	Human Colonic Epithelial Cells	79:108	Cathelicidin Mediates a Protective Role of Vitamin D in Ulcerative Colitis and Human Colonic Epithelial Cells.
31955203	5	32	theme	cathelicidin	747:758	arg1	production					733:742	production	733:742	production of cathelicidin and cytokines	733:772	Human colon cells were treated with 1,25(OH)2D and production of cathelicidin and cytokines were quantified.
31955203	14	33	theme	human	1760:1764	arg1	cells					1785:1789	human colonic epithelial cells	1760:1789	human colonic epithelial cells	1760:1789	Cathelicidin is induced by vitamin D in human colonic epithelial cells and promotes antimicrobial activity against E. coli.
31955203	4	34	dep	METHODS	553:559	arg1	Serum					561:565	Serum	561:565	Serum	561:565	METHODS Serum and colonic cathelicidin levels were measured in UC patients and correlated with clinical and histologic outcomes.
31955203	4	34	dep	METHODS	553:559	arg1	levels					592:597	colonic cathelicidin levels	571:597	colonic cathelicidin levels	571:597	METHODS Serum and colonic cathelicidin levels were measured in UC patients and correlated with clinical and histologic outcomes.
31955203	9	35	theme	decreased	1148:1156	arg1	risk					1158:1161	decreased risk	1148:1161	decreased risk of histologic inflammation and clinical relapse	1148:1209	Higher serum cathelicidin is associated with decreased risk of histologic inflammation and clinical relapse but not independent of 25(OH)D or baseline inflammation.
31955203	5	36	theme	cytokines	764:772	arg1	production					733:742	production	733:742	production of cathelicidin and cytokines	733:772	Human colon cells were treated with 1,25(OH)2D and production of cathelicidin and cytokines were quantified.
31955203	12	37	from	impact	1562:1567	arg1	composition					1593:1603	microbial composition	1583:1603	microbial composition	1583:1603	Intrarectal cathelicidin reduced the severity of DSS colitis but did not mitigate the impact of colitis on microbial composition.
31955203	3	38	theme	D	414:414	arg1	induction					416:424	vitamin D induction	406:424	vitamin D induction of cathelicidin in human colon cells	406:461	Our goal was to evaluate the link between cathelicidin and vitamin D-associated clinical outcomes in UC patients, explore vitamin D induction of cathelicidin in human colon cells, and evaluate the effects of intrarectal human cathelicidin on a murine model of colitis.
31955203	7	39	from	effects	941:947	arg1	colitis					956:962	DSS colitis	952:962	DSS colitis	952:962	Mice were treated with intrarectal cathelicidin, and its effects on DSS colitis and intestinal microbiota were evaluated.
31955203	7	39	from	effects	941:947	arg1	microbiota					979:988	intestinal microbiota	968:988	intestinal microbiota	968:988	Mice were treated with intrarectal cathelicidin, and its effects on DSS colitis and intestinal microbiota were evaluated.
31955203	8	40	theme	OH	1039:1040	arg1	D					1042:1042	serum 25(OH)D	1030:1042	serum 25(OH)D	1030:1042	RESULTS In UC patients, serum 25(OH)D positively correlated with serum and colonic cathelicidin.
31955203	3	41	theme	clinical	364:371	arg1	outcomes					373:380	vitamin D-associated clinical outcomes	343:380	vitamin D-associated clinical outcomes in UC patients	343:395	Our goal was to evaluate the link between cathelicidin and vitamin D-associated clinical outcomes in UC patients, explore vitamin D induction of cathelicidin in human colon cells, and evaluate the effects of intrarectal human cathelicidin on a murine model of colitis.
31955203	3	42	from	induction	416:424	arg1	cells					457:461	human colon cells	445:461	human colon cells	445:461	Our goal was to evaluate the link between cathelicidin and vitamin D-associated clinical outcomes in UC patients, explore vitamin D induction of cathelicidin in human colon cells, and evaluate the effects of intrarectal human cathelicidin on a murine model of colitis.
31955203	3	43	theme	colon	451:455	arg1	cells					457:461	human colon cells	445:461	human colon cells	445:461	Our goal was to evaluate the link between cathelicidin and vitamin D-associated clinical outcomes in UC patients, explore vitamin D induction of cathelicidin in human colon cells, and evaluate the effects of intrarectal human cathelicidin on a murine model of colitis.
31955203	0	44	theme	Vitamin	43:49	arg1	D					51:51	Vitamin D	43:51	Vitamin D	43:51	Cathelicidin Mediates a Protective Role of Vitamin D in Ulcerative Colitis and Human Colonic Epithelial Cells.
31955203	6	45	theme	Antimicrobial	791:803	arg1	activity					805:812	Antimicrobial activity	791:812	Antimicrobial activity against Escherichia coli from cell culture supernatants	791:868	Antimicrobial activity against Escherichia coli from cell culture supernatants was measured.
31955203	1	46	theme	unclear	200:206	arg1	mechanisms					208:217	unclear mechanisms	200:217	unclear mechanisms	200:217	BACKGROUND Vitamin D plays a protective role in ulcerative colitis (UC) patients through unclear mechanisms.
31955203	9	47	theme	Higher	1103:1108	arg1	cathelicidin					1116:1127	Higher serum cathelicidin	1103:1127	Higher serum cathelicidin	1103:1127	Higher serum cathelicidin is associated with decreased risk of histologic inflammation and clinical relapse but not independent of 25(OH)D or baseline inflammation.
31955203	8	48	theme	colonic	1081:1087	arg1	cathelicidin					1089:1100	colonic cathelicidin	1081:1100	colonic cathelicidin	1081:1100	RESULTS In UC patients, serum 25(OH)D positively correlated with serum and colonic cathelicidin.
31955203	15	49	theme	potential	1917:1925	arg1	target					1939:1944	a potential therapeutic target	1915:1944	a potential therapeutic target	1915:1944	Our study provides insights into the vitamin D-cathelicidin pathway as a potential therapeutic target.
31955203	15	49	theme	potential	1917:1925	arg1	insights					1863:1870	insights	1863:1870	insights into the vitamin D-cathelicidin pathway	1863:1910	Our study provides insights into the vitamin D-cathelicidin pathway as a potential therapeutic target.
31955203	3	50	theme	intrarectal	492:502	arg1	cathelicidin					510:521	intrarectal human cathelicidin	492:521	intrarectal human cathelicidin	492:521	Our goal was to evaluate the link between cathelicidin and vitamin D-associated clinical outcomes in UC patients, explore vitamin D induction of cathelicidin in human colon cells, and evaluate the effects of intrarectal human cathelicidin on a murine model of colitis.
31955203	14	51	theme	epithelial	1774:1783	arg1	cells					1785:1789	human colonic epithelial cells	1760:1789	human colonic epithelial cells	1760:1789	Cathelicidin is induced by vitamin D in human colonic epithelial cells and promotes antimicrobial activity against E. coli.
31955203	6	52	from	supernatants	857:868	arg1	activity					805:812	Antimicrobial activity	791:812	Antimicrobial activity against Escherichia coli from cell culture supernatants	791:868	Antimicrobial activity against Escherichia coli from cell culture supernatants was measured.
31955203	10	53	theme	colon	1296:1300	arg1	cells					1302:1306	colon cells	1296:1306	colon cells	1296:1306	The 1,25(OH)2D treatment of colon cells induced cathelicidin and IL-10, repressed TNF-α, and suppressed Escherichia coli growth.
31955203	6	54	theme	cell	844:847	arg1	supernatants					857:868	cell culture supernatants	844:868	cell culture supernatants	844:868	Antimicrobial activity against Escherichia coli from cell culture supernatants was measured.
31955203	1	55	theme	colitis	170:176	arg1	patients					183:190	ulcerative colitis (UC) patients	159:190	ulcerative colitis (UC) patients	159:190	BACKGROUND Vitamin D plays a protective role in ulcerative colitis (UC) patients through unclear mechanisms.
31955203	9	56	theme	inflammation	1177:1188	arg1	risk					1158:1161	decreased risk	1148:1161	decreased risk of histologic inflammation and clinical relapse	1148:1209	Higher serum cathelicidin is associated with decreased risk of histologic inflammation and clinical relapse but not independent of 25(OH)D or baseline inflammation.
31955203	3	57	theme	cathelicidin	510:521	arg1	effects					481:487	the effects	477:487	the effects of intrarectal human cathelicidin on a murine model of colitis	477:550	Our goal was to evaluate the link between cathelicidin and vitamin D-associated clinical outcomes in UC patients, explore vitamin D induction of cathelicidin in human colon cells, and evaluate the effects of intrarectal human cathelicidin on a murine model of colitis.
31955203	4	58	theme	cathelicidin	579:590	arg1	levels					592:597	colonic cathelicidin levels	571:597	colonic cathelicidin levels	571:597	METHODS Serum and colonic cathelicidin levels were measured in UC patients and correlated with clinical and histologic outcomes.
31955203	13	59	theme	D-associated	1664:1675	arg1	outcomes					1691:1698	25(OH)D-associated UC histologic outcomes	1658:1698	25(OH)D-associated UC histologic outcomes	1658:1698	CONCLUSIONS Cathelicidin plays a protective role in 25(OH)D-associated UC histologic outcomes and murine colitis.
31955203	9	60	theme	clinical	1194:1201	arg1	relapse					1203:1209	clinical relapse	1194:1209	clinical relapse	1194:1209	Higher serum cathelicidin is associated with decreased risk of histologic inflammation and clinical relapse but not independent of 25(OH)D or baseline inflammation.
31955203	3	61	theme	colitis	544:550	arg1	model					535:539	a murine model	526:539	a murine model of colitis	526:550	Our goal was to evaluate the link between cathelicidin and vitamin D-associated clinical outcomes in UC patients, explore vitamin D induction of cathelicidin in human colon cells, and evaluate the effects of intrarectal human cathelicidin on a murine model of colitis.
31955203	13	62	theme	histologic	1680:1689	arg1	outcomes					1691:1698	25(OH)D-associated UC histologic outcomes	1658:1698	25(OH)D-associated UC histologic outcomes	1658:1698	CONCLUSIONS Cathelicidin plays a protective role in 25(OH)D-associated UC histologic outcomes and murine colitis.
31955203	5	63	theme	colon	688:692	arg1	cells					694:698	Human colon cells	682:698	Human colon cells	682:698	Human colon cells were treated with 1,25(OH)2D and production of cathelicidin and cytokines were quantified.
31955203	4	64	theme	histologic	661:670	arg1	outcomes					672:679	clinical and histologic outcomes	648:679	clinical and histologic outcomes	648:679	METHODS Serum and colonic cathelicidin levels were measured in UC patients and correlated with clinical and histologic outcomes.
31955203	7	65	theme	intestinal	968:977	arg1	microbiota					979:988	intestinal microbiota	968:988	intestinal microbiota	968:988	Mice were treated with intrarectal cathelicidin, and its effects on DSS colitis and intestinal microbiota were evaluated.
31955203	1	66	theme	Vitamin	122:128	arg1	D					130:130	BACKGROUND Vitamin D	111:130	BACKGROUND Vitamin D	111:130	BACKGROUND Vitamin D plays a protective role in ulcerative colitis (UC) patients through unclear mechanisms.
31955203	0	67	theme	Ulcerative	56:65	arg1	Colitis					67:73	Ulcerative Colitis	56:73	Ulcerative Colitis	56:73	Cathelicidin Mediates a Protective Role of Vitamin D in Ulcerative Colitis and Human Colonic Epithelial Cells.
31955203	9	68	theme	25	1234:1235	arg1	D					1240:1240	25(OH)D	1234:1240	25(OH)D	1234:1240	Higher serum cathelicidin is associated with decreased risk of histologic inflammation and clinical relapse but not independent of 25(OH)D or baseline inflammation.
31955203	12	69	theme	DSS	1525:1527	arg1	colitis					1529:1535	DSS colitis	1525:1535	DSS colitis	1525:1535	Intrarectal cathelicidin reduced the severity of DSS colitis but did not mitigate the impact of colitis on microbial composition.
31955203	11	70	theme	siRNA-cathelicidin	1443:1460	arg1	transfection					1462:1473	siRNA-cathelicidin transfection	1443:1473	siRNA-cathelicidin transfection	1443:1473	This antimicrobial effect was attenuated with siRNA-cathelicidin transfection.
31955203	3	71	from	outcomes	373:380	arg1	patients					388:395	UC patients	385:395	UC patients	385:395	Our goal was to evaluate the link between cathelicidin and vitamin D-associated clinical outcomes in UC patients, explore vitamin D induction of cathelicidin in human colon cells, and evaluate the effects of intrarectal human cathelicidin on a murine model of colitis.
31955203	1	72	theme	protective	140:149	arg1	role					151:154	a protective role	138:154	a protective role	138:154	BACKGROUND Vitamin D plays a protective role in ulcerative colitis (UC) patients through unclear mechanisms.
31955203	3	73	from	effects	481:487	arg1	model					535:539	a murine model	526:539	a murine model of colitis	526:550	Our goal was to evaluate the link between cathelicidin and vitamin D-associated clinical outcomes in UC patients, explore vitamin D induction of cathelicidin in human colon cells, and evaluate the effects of intrarectal human cathelicidin on a murine model of colitis.
31955203	12	74	theme	Intrarectal	1476:1486	arg1	cathelicidin					1488:1499	Intrarectal cathelicidin	1476:1499	Intrarectal cathelicidin	1476:1499	Intrarectal cathelicidin reduced the severity of DSS colitis but did not mitigate the impact of colitis on microbial composition.
31955203	4	75	theme	clinical	648:655	arg1	outcomes					672:679	clinical and histologic outcomes	648:679	clinical and histologic outcomes	648:679	METHODS Serum and colonic cathelicidin levels were measured in UC patients and correlated with clinical and histologic outcomes.
31955203	0	76	theme	Colonic	85:91	arg1	Cells					104:108	Human Colonic Epithelial Cells	79:108	Human Colonic Epithelial Cells	79:108	Cathelicidin Mediates a Protective Role of Vitamin D in Ulcerative Colitis and Human Colonic Epithelial Cells.
31955203	9	77	theme	D	1240:1240	arg1	independent					1219:1229	independent	1219:1229	independent	1219:1229	Higher serum cathelicidin is associated with decreased risk of histologic inflammation and clinical relapse but not independent of 25(OH)D or baseline inflammation.
31955203	9	78	theme	baseline	1245:1252	arg1	inflammation					1254:1265	baseline inflammation	1245:1265	baseline inflammation	1245:1265	Higher serum cathelicidin is associated with decreased risk of histologic inflammation and clinical relapse but not independent of 25(OH)D or baseline inflammation.
31955203	13	79	theme	protective	1639:1648	arg1	role					1650:1653	a protective role	1637:1653	a protective role	1637:1653	CONCLUSIONS Cathelicidin plays a protective role in 25(OH)D-associated UC histologic outcomes and murine colitis.
31955203	3	80	theme	vitamin	406:412	arg1	D					414:414	vitamin D	406:414	vitamin D induction of cathelicidin in human colon cells	406:461	Our goal was to evaluate the link between cathelicidin and vitamin D-associated clinical outcomes in UC patients, explore vitamin D induction of cathelicidin in human colon cells, and evaluate the effects of intrarectal human cathelicidin on a murine model of colitis.
31955203	8	81	theme	serum	1030:1034	arg1	D					1042:1042	serum 25(OH)D	1030:1042	serum 25(OH)D	1030:1042	RESULTS In UC patients, serum 25(OH)D positively correlated with serum and colonic cathelicidin.
31955203	7	82	theme	intrarectal	907:917	arg1	cathelicidin					919:930	intrarectal cathelicidin	907:930	intrarectal cathelicidin	907:930	Mice were treated with intrarectal cathelicidin, and its effects on DSS colitis and intestinal microbiota were evaluated.
31955203	3	83	theme	D-associated	351:362	arg1	outcomes					373:380	vitamin D-associated clinical outcomes	343:380	vitamin D-associated clinical outcomes in UC patients	343:395	Our goal was to evaluate the link between cathelicidin and vitamin D-associated clinical outcomes in UC patients, explore vitamin D induction of cathelicidin in human colon cells, and evaluate the effects of intrarectal human cathelicidin on a murine model of colitis.
31955203	10	84	theme	Escherichia	1372:1382	arg1	growth					1389:1394	Escherichia coli growth	1372:1394	Escherichia coli growth	1372:1394	The 1,25(OH)2D treatment of colon cells induced cathelicidin and IL-10, repressed TNF-α, and suppressed Escherichia coli growth.
31955203	0	85	theme	Protective	24:33	arg1	Role					35:38	a Protective Role	22:38	a Protective Role of Vitamin D in Ulcerative Colitis and Human Colonic Epithelial Cells	22:108	Cathelicidin Mediates a Protective Role of Vitamin D in Ulcerative Colitis and Human Colonic Epithelial Cells.
31955203	12	86	theme	colitis	1572:1578	arg1	impact					1562:1567	the impact	1558:1567	the impact of colitis on microbial composition	1558:1603	Intrarectal cathelicidin reduced the severity of DSS colitis but did not mitigate the impact of colitis on microbial composition.
31955203	13	87	theme	CONCLUSIONS	1606:1616	arg1	Cathelicidin					1618:1629	CONCLUSIONS Cathelicidin	1606:1629	CONCLUSIONS Cathelicidin	1606:1629	CONCLUSIONS Cathelicidin plays a protective role in 25(OH)D-associated UC histologic outcomes and murine colitis.
31955203	3	88	theme	cathelicidin	429:440	arg1	induction					416:424	vitamin D induction	406:424	vitamin D induction of cathelicidin in human colon cells	406:461	Our goal was to evaluate the link between cathelicidin and vitamin D-associated clinical outcomes in UC patients, explore vitamin D induction of cathelicidin in human colon cells, and evaluate the effects of intrarectal human cathelicidin on a murine model of colitis.
31955203	14	89	theme	vitamin	1747:1753	arg1	D					1755:1755	vitamin D	1747:1755	vitamin D	1747:1755	Cathelicidin is induced by vitamin D in human colonic epithelial cells and promotes antimicrobial activity against E. coli.
31955203	12	90	theme	microbial	1583:1591	arg1	composition					1593:1603	microbial composition	1583:1603	microbial composition	1583:1603	Intrarectal cathelicidin reduced the severity of DSS colitis but did not mitigate the impact of colitis on microbial composition.
31955203	3	91	theme	UC	385:386	arg1	patients					388:395	UC patients	385:395	UC patients	385:395	Our goal was to evaluate the link between cathelicidin and vitamin D-associated clinical outcomes in UC patients, explore vitamin D induction of cathelicidin in human colon cells, and evaluate the effects of intrarectal human cathelicidin on a murine model of colitis.
31955203	0	92	theme	D	51:51	arg1	Role					35:38	a Protective Role	22:38	a Protective Role of Vitamin D in Ulcerative Colitis and Human Colonic Epithelial Cells	22:108	Cathelicidin Mediates a Protective Role of Vitamin D in Ulcerative Colitis and Human Colonic Epithelial Cells.
31955203	9	93	theme	serum	1110:1114	arg1	cathelicidin					1116:1127	Higher serum cathelicidin	1103:1127	Higher serum cathelicidin	1103:1127	Higher serum cathelicidin is associated with decreased risk of histologic inflammation and clinical relapse but not independent of 25(OH)D or baseline inflammation.
31955203	3	94	theme	human	445:449	arg1	cells					457:461	human colon cells	445:461	human colon cells	445:461	Our goal was to evaluate the link between cathelicidin and vitamin D-associated clinical outcomes in UC patients, explore vitamin D induction of cathelicidin in human colon cells, and evaluate the effects of intrarectal human cathelicidin on a murine model of colitis.
31955203	4	95	theme	colonic	571:577	arg1	levels					592:597	colonic cathelicidin levels	571:597	colonic cathelicidin levels	571:597	METHODS Serum and colonic cathelicidin levels were measured in UC patients and correlated with clinical and histologic outcomes.
31955203	10	96	theme	2D	1280:1281	arg1	treatment					1283:1291	The 1,25(OH)2D treatment	1268:1291	The 1,25(OH)2D treatment of colon cells	1268:1306	The 1,25(OH)2D treatment of colon cells induced cathelicidin and IL-10, repressed TNF-α, and suppressed Escherichia coli growth.
31951191	10	0	theme	cellular	1304:1311	arg1	iso-C15					1272:1278	iso-C15 : 0	1272:1282	iso-C15 : 0	1272:1282	Results of fatty acid methyl ester analysis revealed that iso-C15 : 0 was the predominant cellular fatty acid.
31951191	10	0	theme	cellular	1304:1311	arg1	acid					1319:1322	the predominant cellular fatty acid	1288:1322	the predominant cellular fatty acid	1288:1322	Results of fatty acid methyl ester analysis revealed that iso-C15 : 0 was the predominant cellular fatty acid.
31951191	6	1	theme	16S	514:516	arg1	similarity					537:546	16S rRNA gene sequence similarity	514:546	16S rRNA gene sequence similarity	514:546	Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that this strain was most closely related to Bacillus lehensis MLB2T (99.4 %), Bacillus oshimensis K11T (98.8 %) and Bacillus patagoniensis PAT 05T (96.6 %).
31951191	2	2	theme	novel	107:111	arg1	strain					123:128	A novel bacterial strain	105:128	A novel bacterial strain	105:128	A novel bacterial strain, designated AK13T (=KACC 21401T=DSM 109981T), was isolated from the rhizosphere of Miscanthus sacchariflorus.
31951191	5	3	theme	DNA	422:424	arg1	%					480:480	40 mol%	474:480	40 mol%	474:480	The genomic DNA G+C content of strain AK13T was estimated to be 40 mol%.
31951191	5	3	theme	DNA	422:424	arg1	content					430:436	The genomic DNA G+C content	410:436	The genomic DNA G+C content of strain AK13T	410:452	The genomic DNA G+C content of strain AK13T was estimated to be 40 mol%.
31951191	9	4	theme	isoprenoid	1185:1194	arg1	MK-7					1208:1211	MK-7	1208:1211	MK-7	1208:1211	The cell wall of strain AK13T contained meso-diaminopimelic acid, and the major isoprenoid quinone was MK-7.
31951191	9	4	theme	isoprenoid	1185:1194	arg1	quinone					1196:1202	the major isoprenoid quinone	1175:1202	the major isoprenoid quinone	1175:1202	The cell wall of strain AK13T contained meso-diaminopimelic acid, and the major isoprenoid quinone was MK-7.
31951191	4	5	theme	smooth	386:391	arg1	surfaces					400:407	smooth convex surfaces	386:407	smooth convex surfaces	386:407	It formed yellow circular colonies with smooth convex surfaces.
31951191	5	6	theme	40	474:475	arg1	mol					477:479	mol	477:479	mol	477:479	The genomic DNA G+C content of strain AK13T was estimated to be 40 mol%.
31951191	3	7	theme	Strain	240:245	arg1	bacterium					335:343	an aerobic, Gram-stain-positive, endospore-forming and rod-shaped bacterium	269:343	an aerobic, Gram-stain-positive, endospore-forming and rod-shaped bacterium	269:343	Strain AK13T was found to be an aerobic, Gram-stain-positive, endospore-forming and rod-shaped bacterium.
31951191	3	7	theme	Strain	240:245	arg1	AK13T					247:251	Strain AK13T	240:251	Strain AK13T	240:251	Strain AK13T was found to be an aerobic, Gram-stain-positive, endospore-forming and rod-shaped bacterium.
31951191	5	8	theme	genomic	414:420	arg1	%					480:480	40 mol%	474:480	40 mol%	474:480	The genomic DNA G+C content of strain AK13T was estimated to be 40 mol%.
31951191	5	8	theme	genomic	414:420	arg1	content					430:436	The genomic DNA G+C content	410:436	The genomic DNA G+C content of strain AK13T	410:452	The genomic DNA G+C content of strain AK13T was estimated to be 40 mol%.
31951191	6	9	theme	Phylogenetic	483:494	arg1	analysis					496:503	Phylogenetic analysis	483:503	Phylogenetic analysis based on 16S rRNA gene sequence similarity	483:546	Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that this strain was most closely related to Bacillus lehensis MLB2T (99.4 %), Bacillus oshimensis K11T (98.8 %) and Bacillus patagoniensis PAT 05T (96.6 %).
31951191	11	10	theme	thin-layer	1341:1350	arg1	analysis					1367:1374	Two-dimensional thin-layer chromatography analysis	1325:1374	Two-dimensional thin-layer chromatography analysis	1325:1374	Two-dimensional thin-layer chromatography analysis indicated that the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and glycolipid.
31951191	10	11	theme	predominant	1292:1302	arg1	iso-C15					1272:1278	iso-C15 : 0	1272:1282	iso-C15 : 0	1272:1282	Results of fatty acid methyl ester analysis revealed that iso-C15 : 0 was the predominant cellular fatty acid.
31951191	10	11	theme	predominant	1292:1302	arg1	acid					1319:1322	the predominant cellular fatty acid	1288:1322	the predominant cellular fatty acid	1288:1322	Results of fatty acid methyl ester analysis revealed that iso-C15 : 0 was the predominant cellular fatty acid.
31951191	4	12	theme	yellow	356:361	arg1	colonies					372:379	yellow circular colonies	356:379	yellow circular colonies	356:379	It formed yellow circular colonies with smooth convex surfaces.
31951191	8	13	dep	optimum	998:1004	arg1	°C					1013:1014	28-35 °C	1007:1014	28-35 °C	1007:1014	Cells grew at 5-40 °C (optimum, 28-35 °C), pH 6.5-13 (optimum, pH 8-9) and in the presence of 0-13.0 % (w/v) NaCl (optimum, 1 %).
31951191	7	14	theme	18.8	953:956	arg1	%					958:958	42.7, 42.6 and 18.8 %	938:958	%	958:958	The average nucleotide identity values between strain AK13T and B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T were 90.93, 91.05 and 71.87 %, respectively, with the digital DNA-DNA hybridization values of 42.7, 42.6 and 18.8 %, respectively.
31951191	7	15	theme	hybridization	914:926	arg1	values					928:933	the digital DNA-DNA hybridization values	894:933	the digital DNA-DNA hybridization values of 42.7, 42.6 and 18.8 %, respectively	894:972	The average nucleotide identity values between strain AK13T and B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T were 90.93, 91.05 and 71.87 %, respectively, with the digital DNA-DNA hybridization values of 42.7, 42.6 and 18.8 %, respectively.
31951191	7	16	theme	digital	898:904	arg1	values					928:933	the digital DNA-DNA hybridization values	894:933	the digital DNA-DNA hybridization values of 42.7, 42.6 and 18.8 %, respectively	894:972	The average nucleotide identity values between strain AK13T and B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T were 90.93, 91.05 and 71.87 %, respectively, with the digital DNA-DNA hybridization values of 42.7, 42.6 and 18.8 %, respectively.
31951191	6	17	dep	Bacillus	600:607	arg1	lehensis					609:616	lehensis	609:616	lehensis	609:616	Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that this strain was most closely related to Bacillus lehensis MLB2T (99.4 %), Bacillus oshimensis K11T (98.8 %) and Bacillus patagoniensis PAT 05T (96.6 %).
31951191	7	18	theme	42.7	938:941	arg1	%					958:958	42.7, 42.6 and 18.8 %	938:958	%	958:958	The average nucleotide identity values between strain AK13T and B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T were 90.93, 91.05 and 71.87 %, respectively, with the digital DNA-DNA hybridization values of 42.7, 42.6 and 18.8 %, respectively.
31951191	12	19	theme	strain	1566:1571	arg1	AK13T					1573:1577	strain AK13T	1566:1577	strain AK13T	1566:1577	The genotypic and phenotypic characteristics suggested that strain AK13T represented a novel species of the genus Bacillus, and thus the name Bacillus miscanthi sp.
31951191	2	20	theme	sacchariflorus	224:237	arg1	rhizosphere					198:208	the rhizosphere	194:208	the rhizosphere of Miscanthus sacchariflorus	194:237	A novel bacterial strain, designated AK13T (=KACC 21401T=DSM 109981T), was isolated from the rhizosphere of Miscanthus sacchariflorus.
31951191	12	21	theme	genotypic	1510:1518	arg1	characteristics					1535:1549	The genotypic and phenotypic characteristics	1506:1549	The genotypic and phenotypic characteristics	1506:1549	The genotypic and phenotypic characteristics suggested that strain AK13T represented a novel species of the genus Bacillus, and thus the name Bacillus miscanthi sp.
31951191	6	22	theme	Bacillus	600:607	arg1	MLB2T					618:622	Bacillus lehensis MLB2T	600:622	Bacillus lehensis MLB2T (99.4 %)	600:631	Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that this strain was most closely related to Bacillus lehensis MLB2T (99.4 %), Bacillus oshimensis K11T (98.8 %) and Bacillus patagoniensis PAT 05T (96.6 %).
31951191	6	22	theme	Bacillus	600:607	arg1	%					630:630	99.4 %	625:630	99.4 %	625:630	Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that this strain was most closely related to Bacillus lehensis MLB2T (99.4 %), Bacillus oshimensis K11T (98.8 %) and Bacillus patagoniensis PAT 05T (96.6 %).
31951191	12	23	theme	phenotypic	1524:1533	arg1	characteristics					1535:1549	The genotypic and phenotypic characteristics	1506:1549	The genotypic and phenotypic characteristics	1506:1549	The genotypic and phenotypic characteristics suggested that strain AK13T represented a novel species of the genus Bacillus, and thus the name Bacillus miscanthi sp.
31951191	10	24	theme	ester	1243:1247	arg1	analysis					1249:1256	fatty acid methyl ester analysis	1225:1256	fatty acid methyl ester analysis	1225:1256	Results of fatty acid methyl ester analysis revealed that iso-C15 : 0 was the predominant cellular fatty acid.
31951191	6	25	dep	Bacillus	634:641	arg1	oshimensis					643:652	oshimensis	643:652	oshimensis	643:652	Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that this strain was most closely related to Bacillus lehensis MLB2T (99.4 %), Bacillus oshimensis K11T (98.8 %) and Bacillus patagoniensis PAT 05T (96.6 %).
31951191	10	26	theme	acid	1231:1234	arg1	ester					1243:1247	fatty acid methyl ester	1225:1247	fatty acid methyl ester analysis	1225:1256	Results of fatty acid methyl ester analysis revealed that iso-C15 : 0 was the predominant cellular fatty acid.
31951191	0	27	theme	Bacillus	0:7	arg1	sp					19:20	Bacillus miscanthi sp	0:20	Bacillus miscanthi sp.	0:21	Bacillus miscanthi sp.
31951191	7	28	theme	identity	736:743	arg1	%					872:872	90.93, 91.05 and 71.87 %	849:872	%	872:872	The average nucleotide identity values between strain AK13T and B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T were 90.93, 91.05 and 71.87 %, respectively, with the digital DNA-DNA hybridization values of 42.7, 42.6 and 18.8 %, respectively.
31951191	7	28	theme	identity	736:743	arg1	values					745:750	The average nucleotide identity values	713:750	The average nucleotide identity values between strain AK13T and B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T	713:842	The average nucleotide identity values between strain AK13T and B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T were 90.93, 91.05 and 71.87 %, respectively, with the digital DNA-DNA hybridization values of 42.7, 42.6 and 18.8 %, respectively.
31951191	1	29	theme	Miscanthus	78:87	arg1	sacchariflorus					89:102	Miscanthus sacchariflorus	78:102	Miscanthus sacchariflorus	78:102	nov., a alkaliphilic bacterium from the rhizosphere of Miscanthus sacchariflorus.
31951191	6	30	theme	Bacillus	634:641	arg1	K11T					654:657	Bacillus oshimensis K11T	634:657	Bacillus oshimensis K11T (98.8 %)	634:666	Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that this strain was most closely related to Bacillus lehensis MLB2T (99.4 %), Bacillus oshimensis K11T (98.8 %) and Bacillus patagoniensis PAT 05T (96.6 %).
31951191	6	30	theme	Bacillus	634:641	arg1	%					665:665	98.8 %	660:665	98.8 %	660:665	Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that this strain was most closely related to Bacillus lehensis MLB2T (99.4 %), Bacillus oshimensis K11T (98.8 %) and Bacillus patagoniensis PAT 05T (96.6 %).
31951191	7	31	theme	average	717:723	arg1	%					872:872	90.93, 91.05 and 71.87 %	849:872	%	872:872	The average nucleotide identity values between strain AK13T and B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T were 90.93, 91.05 and 71.87 %, respectively, with the digital DNA-DNA hybridization values of 42.7, 42.6 and 18.8 %, respectively.
31951191	7	31	theme	average	717:723	arg1	values					745:750	The average nucleotide identity values	713:750	The average nucleotide identity values between strain AK13T and B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T	713:842	The average nucleotide identity values between strain AK13T and B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T were 90.93, 91.05 and 71.87 %, respectively, with the digital DNA-DNA hybridization values of 42.7, 42.6 and 18.8 %, respectively.
31951191	9	32	theme	strain	1122:1127	arg1	AK13T					1129:1133	strain AK13T	1122:1133	strain AK13T	1122:1133	The cell wall of strain AK13T contained meso-diaminopimelic acid, and the major isoprenoid quinone was MK-7.
31951191	2	33	theme	=KACC	149:153	arg1	109981T					166:172	=KACC 21401T=DSM 109981T	149:172	=KACC 21401T=DSM 109981T	149:172	A novel bacterial strain, designated AK13T (=KACC 21401T=DSM 109981T), was isolated from the rhizosphere of Miscanthus sacchariflorus.
31951191	2	33	theme	=KACC	149:153	arg1	AK13T					142:146	AK13T	142:146	AK13T (=KACC 21401T=DSM 109981T)	142:173	A novel bacterial strain, designated AK13T (=KACC 21401T=DSM 109981T), was isolated from the rhizosphere of Miscanthus sacchariflorus.
31951191	12	34	theme	miscanthi	1657:1665	arg1	sp					1667:1668	Bacillus miscanthi sp	1648:1668	the name Bacillus miscanthi sp	1639:1668	The genotypic and phenotypic characteristics suggested that strain AK13T represented a novel species of the genus Bacillus, and thus the name Bacillus miscanthi sp.
31951191	8	35	dep	optimum	1029:1035	arg1	pH					1038:1039	pH 8-9	1038:1043	pH 8-9	1038:1043	Cells grew at 5-40 °C (optimum, 28-35 °C), pH 6.5-13 (optimum, pH 8-9) and in the presence of 0-13.0 % (w/v) NaCl (optimum, 1 %).
31951191	6	36	theme	PAT	695:697	arg1	05T					699:701	Bacillus patagoniensis PAT 05T	672:701	Bacillus patagoniensis PAT 05T (96.6 %)	672:710	Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that this strain was most closely related to Bacillus lehensis MLB2T (99.4 %), Bacillus oshimensis K11T (98.8 %) and Bacillus patagoniensis PAT 05T (96.6 %).
31951191	6	36	theme	PAT	695:697	arg1	%					709:709	96.6 %	704:709	96.6 %	704:709	Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that this strain was most closely related to Bacillus lehensis MLB2T (99.4 %), Bacillus oshimensis K11T (98.8 %) and Bacillus patagoniensis PAT 05T (96.6 %).
31951191	8	37	dep	optimum	1090:1096	arg1	%					1101:1101	1 %	1099:1101	1 %	1099:1101	Cells grew at 5-40 °C (optimum, 28-35 °C), pH 6.5-13 (optimum, pH 8-9) and in the presence of 0-13.0 % (w/v) NaCl (optimum, 1 %).
31951191	6	38	theme	sequence	528:535	arg1	similarity					537:546	16S rRNA gene sequence similarity	514:546	16S rRNA gene sequence similarity	514:546	Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that this strain was most closely related to Bacillus lehensis MLB2T (99.4 %), Bacillus oshimensis K11T (98.8 %) and Bacillus patagoniensis PAT 05T (96.6 %).
31951191	9	39	contain	contained	1135:1143	arg1	wall					1114:1117	The cell wall	1105:1117	The cell wall of strain AK13T	1105:1133	The cell wall of strain AK13T contained meso-diaminopimelic acid, and the major isoprenoid quinone was MK-7.
31951191	9	39	contain	contained	1135:1143	arg2	acid					1165:1168	meso-diaminopimelic acid	1145:1168	meso-diaminopimelic acid	1145:1168	The cell wall of strain AK13T contained meso-diaminopimelic acid, and the major isoprenoid quinone was MK-7.
31951191	7	40	theme	patagoniensis	822:834	arg1	05T					840:842	B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T	777:842	B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T	777:842	The average nucleotide identity values between strain AK13T and B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T were 90.93, 91.05 and 71.87 %, respectively, with the digital DNA-DNA hybridization values of 42.7, 42.6 and 18.8 %, respectively.
31951191	1	41	theme	alkaliphilic	31:42	arg1	bacterium					44:52	a alkaliphilic bacterium	29:52	a alkaliphilic bacterium from the rhizosphere of Miscanthus sacchariflorus	29:102	nov., a alkaliphilic bacterium from the rhizosphere of Miscanthus sacchariflorus.
31951191	1	41	theme	alkaliphilic	31:42	arg1	nov.					23:26	nov.	23:26	nov.	23:26	nov., a alkaliphilic bacterium from the rhizosphere of Miscanthus sacchariflorus.
31951191	6	42	theme	rRNA	518:521	arg1	similarity					537:546	16S rRNA gene sequence similarity	514:546	16S rRNA gene sequence similarity	514:546	Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that this strain was most closely related to Bacillus lehensis MLB2T (99.4 %), Bacillus oshimensis K11T (98.8 %) and Bacillus patagoniensis PAT 05T (96.6 %).
31951191	2	43	theme	bacterial	113:121	arg1	strain					123:128	A novel bacterial strain	105:128	A novel bacterial strain	105:128	A novel bacterial strain, designated AK13T (=KACC 21401T=DSM 109981T), was isolated from the rhizosphere of Miscanthus sacchariflorus.
31951191	11	44	theme	polar	1401:1405	arg1	diphosphatidylglycerol					1419:1440	diphosphatidylglycerol	1419:1440	diphosphatidylglycerol	1419:1440	Two-dimensional thin-layer chromatography analysis indicated that the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and glycolipid.
31951191	11	44	theme	polar	1401:1405	arg1	lipids					1407:1412	the major polar lipids	1391:1412	the major polar lipids	1391:1412	Two-dimensional thin-layer chromatography analysis indicated that the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and glycolipid.
31951191	1	45	from	rhizosphere	63:73	arg1	bacterium					44:52	a alkaliphilic bacterium	29:52	a alkaliphilic bacterium from the rhizosphere of Miscanthus sacchariflorus	29:102	nov., a alkaliphilic bacterium from the rhizosphere of Miscanthus sacchariflorus.
31951191	1	45	from	rhizosphere	63:73	arg1	nov.					23:26	nov.	23:26	nov.	23:26	nov., a alkaliphilic bacterium from the rhizosphere of Miscanthus sacchariflorus.
31951191	9	46	theme	major	1179:1183	arg1	MK-7					1208:1211	MK-7	1208:1211	MK-7	1208:1211	The cell wall of strain AK13T contained meso-diaminopimelic acid, and the major isoprenoid quinone was MK-7.
31951191	9	46	theme	major	1179:1183	arg1	quinone					1196:1202	the major isoprenoid quinone	1175:1202	the major isoprenoid quinone	1175:1202	The cell wall of strain AK13T contained meso-diaminopimelic acid, and the major isoprenoid quinone was MK-7.
31951191	4	47	theme	convex	393:398	arg1	surfaces					400:407	smooth convex surfaces	386:407	smooth convex surfaces	386:407	It formed yellow circular colonies with smooth convex surfaces.
31951191	5	48	theme	G+C	426:428	arg1	%					480:480	40 mol%	474:480	40 mol%	474:480	The genomic DNA G+C content of strain AK13T was estimated to be 40 mol%.
31951191	5	48	theme	G+C	426:428	arg1	content					430:436	The genomic DNA G+C content	410:436	The genomic DNA G+C content of strain AK13T	410:452	The genomic DNA G+C content of strain AK13T was estimated to be 40 mol%.
31951191	12	49	theme	genus	1614:1618	arg1	species					1599:1605	a novel species	1591:1605	a novel species of the genus Bacillus	1591:1627	The genotypic and phenotypic characteristics suggested that strain AK13T represented a novel species of the genus Bacillus, and thus the name Bacillus miscanthi sp.
31951191	5	50	theme	mol	477:479	arg1	%					480:480	40 mol%	474:480	40 mol%	474:480	The genomic DNA G+C content of strain AK13T was estimated to be 40 mol%.
31951191	5	50	theme	mol	477:479	arg1	content					430:436	The genomic DNA G+C content	410:436	The genomic DNA G+C content of strain AK13T	410:452	The genomic DNA G+C content of strain AK13T was estimated to be 40 mol%.
31951191	4	51	theme	circular	363:370	arg1	colonies					372:379	yellow circular colonies	356:379	yellow circular colonies	356:379	It formed yellow circular colonies with smooth convex surfaces.
31951191	11	52	theme	chromatography	1352:1365	arg1	analysis					1367:1374	Two-dimensional thin-layer chromatography analysis	1325:1374	Two-dimensional thin-layer chromatography analysis	1325:1374	Two-dimensional thin-layer chromatography analysis indicated that the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and glycolipid.
31951191	12	53	theme	novel	1593:1597	arg1	species					1599:1605	a novel species	1591:1605	a novel species of the genus Bacillus	1591:1627	The genotypic and phenotypic characteristics suggested that strain AK13T represented a novel species of the genus Bacillus, and thus the name Bacillus miscanthi sp.
31951191	7	54	theme	%	958:958	arg1	values					928:933	the digital DNA-DNA hybridization values	894:933	the digital DNA-DNA hybridization values of 42.7, 42.6 and 18.8 %, respectively	894:972	The average nucleotide identity values between strain AK13T and B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T were 90.93, 91.05 and 71.87 %, respectively, with the digital DNA-DNA hybridization values of 42.7, 42.6 and 18.8 %, respectively.
31951191	11	55	theme	Two-dimensional	1325:1339	arg1	analysis					1367:1374	Two-dimensional thin-layer chromatography analysis	1325:1374	Two-dimensional thin-layer chromatography analysis	1325:1374	Two-dimensional thin-layer chromatography analysis indicated that the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and glycolipid.
31951191	8	56	theme	%	1076:1076	arg1	optimum					1090:1096	optimum	1090:1096	optimum	1090:1096	Cells grew at 5-40 °C (optimum, 28-35 °C), pH 6.5-13 (optimum, pH 8-9) and in the presence of 0-13.0 % (w/v) NaCl (optimum, 1 %).
31951191	8	56	theme	%	1076:1076	arg1	NaCl					1084:1087	0-13.0 % (w/v) NaCl	1069:1087	0-13.0 % (w/v) NaCl (optimum, 1 %)	1069:1102	Cells grew at 5-40 °C (optimum, 28-35 °C), pH 6.5-13 (optimum, pH 8-9) and in the presence of 0-13.0 % (w/v) NaCl (optimum, 1 %).
31951191	2	57	theme	Miscanthus	213:222	arg1	sacchariflorus					224:237	Miscanthus sacchariflorus	213:237	Miscanthus sacchariflorus	213:237	A novel bacterial strain, designated AK13T (=KACC 21401T=DSM 109981T), was isolated from the rhizosphere of Miscanthus sacchariflorus.
31951191	7	58	theme	strain	760:765	arg1	AK13T					767:771	strain AK13T	760:771	strain AK13T	760:771	The average nucleotide identity values between strain AK13T and B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T were 90.93, 91.05 and 71.87 %, respectively, with the digital DNA-DNA hybridization values of 42.7, 42.6 and 18.8 %, respectively.
31951191	8	59	theme	w/v	1079:1081	arg1	optimum					1090:1096	optimum	1090:1096	optimum	1090:1096	Cells grew at 5-40 °C (optimum, 28-35 °C), pH 6.5-13 (optimum, pH 8-9) and in the presence of 0-13.0 % (w/v) NaCl (optimum, 1 %).
31951191	8	59	theme	w/v	1079:1081	arg1	NaCl					1084:1087	0-13.0 % (w/v) NaCl	1069:1087	0-13.0 % (w/v) NaCl (optimum, 1 %)	1069:1102	Cells grew at 5-40 °C (optimum, 28-35 °C), pH 6.5-13 (optimum, pH 8-9) and in the presence of 0-13.0 % (w/v) NaCl (optimum, 1 %).
31951191	7	60	with	%	872:872	arg1	values					928:933	the digital DNA-DNA hybridization values	894:933	the digital DNA-DNA hybridization values of 42.7, 42.6 and 18.8 %, respectively	894:972	The average nucleotide identity values between strain AK13T and B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T were 90.93, 91.05 and 71.87 %, respectively, with the digital DNA-DNA hybridization values of 42.7, 42.6 and 18.8 %, respectively.
31951191	3	61	theme	Gram-stain-positive	281:299	arg1	bacterium					335:343	an aerobic, Gram-stain-positive, endospore-forming and rod-shaped bacterium	269:343	an aerobic, Gram-stain-positive, endospore-forming and rod-shaped bacterium	269:343	Strain AK13T was found to be an aerobic, Gram-stain-positive, endospore-forming and rod-shaped bacterium.
31951191	3	61	theme	Gram-stain-positive	281:299	arg1	AK13T					247:251	Strain AK13T	240:251	Strain AK13T	240:251	Strain AK13T was found to be an aerobic, Gram-stain-positive, endospore-forming and rod-shaped bacterium.
31951191	8	62	theme	NaCl	1084:1087	arg1	presence					1057:1064	the presence	1053:1064	the presence of 0-13.0 % (w/v) NaCl (optimum, 1 %)	1053:1102	Cells grew at 5-40 °C (optimum, 28-35 °C), pH 6.5-13 (optimum, pH 8-9) and in the presence of 0-13.0 % (w/v) NaCl (optimum, 1 %).
31951191	7	63	theme	DNA-DNA	906:912	arg1	values					928:933	the digital DNA-DNA hybridization values	894:933	the digital DNA-DNA hybridization values of 42.7, 42.6 and 18.8 %, respectively	894:972	The average nucleotide identity values between strain AK13T and B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T were 90.93, 91.05 and 71.87 %, respectively, with the digital DNA-DNA hybridization values of 42.7, 42.6 and 18.8 %, respectively.
31951191	3	64	theme	aerobic	272:278	arg1	bacterium					335:343	an aerobic, Gram-stain-positive, endospore-forming and rod-shaped bacterium	269:343	an aerobic, Gram-stain-positive, endospore-forming and rod-shaped bacterium	269:343	Strain AK13T was found to be an aerobic, Gram-stain-positive, endospore-forming and rod-shaped bacterium.
31951191	3	64	theme	aerobic	272:278	arg1	AK13T					247:251	Strain AK13T	240:251	Strain AK13T	240:251	Strain AK13T was found to be an aerobic, Gram-stain-positive, endospore-forming and rod-shaped bacterium.
31951191	3	65	theme	rod-shaped	324:333	arg1	bacterium					335:343	an aerobic, Gram-stain-positive, endospore-forming and rod-shaped bacterium	269:343	an aerobic, Gram-stain-positive, endospore-forming and rod-shaped bacterium	269:343	Strain AK13T was found to be an aerobic, Gram-stain-positive, endospore-forming and rod-shaped bacterium.
31951191	3	65	theme	rod-shaped	324:333	arg1	AK13T					247:251	Strain AK13T	240:251	Strain AK13T	240:251	Strain AK13T was found to be an aerobic, Gram-stain-positive, endospore-forming and rod-shaped bacterium.
31951191	12	66	dep	genus	1614:1618	arg1	Bacillus					1620:1627	the genus Bacillus	1610:1627	the genus Bacillus	1610:1627	The genotypic and phenotypic characteristics suggested that strain AK13T represented a novel species of the genus Bacillus, and thus the name Bacillus miscanthi sp.
31951191	3	67	theme	endospore-forming	302:318	arg1	bacterium					335:343	an aerobic, Gram-stain-positive, endospore-forming and rod-shaped bacterium	269:343	an aerobic, Gram-stain-positive, endospore-forming and rod-shaped bacterium	269:343	Strain AK13T was found to be an aerobic, Gram-stain-positive, endospore-forming and rod-shaped bacterium.
31951191	3	67	theme	endospore-forming	302:318	arg1	AK13T					247:251	Strain AK13T	240:251	Strain AK13T	240:251	Strain AK13T was found to be an aerobic, Gram-stain-positive, endospore-forming and rod-shaped bacterium.
31951191	7	68	theme	71.87	866:870	arg1	%					872:872	90.93, 91.05 and 71.87 %	849:872	%	872:872	The average nucleotide identity values between strain AK13T and B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T were 90.93, 91.05 and 71.87 %, respectively, with the digital DNA-DNA hybridization values of 42.7, 42.6 and 18.8 %, respectively.
31951191	7	68	theme	71.87	866:870	arg1	values					745:750	The average nucleotide identity values	713:750	The average nucleotide identity values between strain AK13T and B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T	713:842	The average nucleotide identity values between strain AK13T and B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T were 90.93, 91.05 and 71.87 %, respectively, with the digital DNA-DNA hybridization values of 42.7, 42.6 and 18.8 %, respectively.
31951191	7	69	theme	B.	819:820	arg1	05T					840:842	B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T	777:842	B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T	777:842	The average nucleotide identity values between strain AK13T and B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T were 90.93, 91.05 and 71.87 %, respectively, with the digital DNA-DNA hybridization values of 42.7, 42.6 and 18.8 %, respectively.
31951191	2	70	attach	isolated	180:187	arg2	strain					123:128	A novel bacterial strain	105:128	A novel bacterial strain	105:128	A novel bacterial strain, designated AK13T (=KACC 21401T=DSM 109981T), was isolated from the rhizosphere of Miscanthus sacchariflorus.
31951191	2	70	attach	isolated	180:187	arg1	rhizosphere					198:208	the rhizosphere	194:208	the rhizosphere of Miscanthus sacchariflorus	194:237	A novel bacterial strain, designated AK13T (=KACC 21401T=DSM 109981T), was isolated from the rhizosphere of Miscanthus sacchariflorus.
31951191	10	71	theme	methyl	1236:1241	arg1	ester					1243:1247	fatty acid methyl ester	1225:1247	fatty acid methyl ester analysis	1225:1256	Results of fatty acid methyl ester analysis revealed that iso-C15 : 0 was the predominant cellular fatty acid.
31951191	0	72	theme	miscanthi	9:17	arg1	sp					19:20	Bacillus miscanthi sp	0:20	Bacillus miscanthi sp.	0:21	Bacillus miscanthi sp.
31951191	10	73	theme	fatty	1225:1229	arg1	ester					1243:1247	fatty acid methyl ester	1225:1247	fatty acid methyl ester analysis	1225:1256	Results of fatty acid methyl ester analysis revealed that iso-C15 : 0 was the predominant cellular fatty acid.
31951191	9	74	theme	cell	1109:1112	arg1	wall					1114:1117	The cell wall	1105:1117	The cell wall of strain AK13T	1105:1133	The cell wall of strain AK13T contained meso-diaminopimelic acid, and the major isoprenoid quinone was MK-7.
31951191	7	75	theme	90.93	849:853	arg1	%					872:872	90.93, 91.05 and 71.87 %	849:872	%	872:872	The average nucleotide identity values between strain AK13T and B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T were 90.93, 91.05 and 71.87 %, respectively, with the digital DNA-DNA hybridization values of 42.7, 42.6 and 18.8 %, respectively.
31951191	7	75	theme	90.93	849:853	arg1	values					745:750	The average nucleotide identity values	713:750	The average nucleotide identity values between strain AK13T and B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T	713:842	The average nucleotide identity values between strain AK13T and B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T were 90.93, 91.05 and 71.87 %, respectively, with the digital DNA-DNA hybridization values of 42.7, 42.6 and 18.8 %, respectively.
31951191	1	76	theme	sacchariflorus	89:102	arg1	rhizosphere					63:73	the rhizosphere	59:73	the rhizosphere of Miscanthus sacchariflorus	59:102	nov., a alkaliphilic bacterium from the rhizosphere of Miscanthus sacchariflorus.
31951191	6	77	theme	Bacillus	672:679	arg1	05T					699:701	Bacillus patagoniensis PAT 05T	672:701	Bacillus patagoniensis PAT 05T (96.6 %)	672:710	Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that this strain was most closely related to Bacillus lehensis MLB2T (99.4 %), Bacillus oshimensis K11T (98.8 %) and Bacillus patagoniensis PAT 05T (96.6 %).
31951191	6	77	theme	Bacillus	672:679	arg1	%					709:709	96.6 %	704:709	96.6 %	704:709	Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that this strain was most closely related to Bacillus lehensis MLB2T (99.4 %), Bacillus oshimensis K11T (98.8 %) and Bacillus patagoniensis PAT 05T (96.6 %).
31951191	12	78	dep	name	1643:1646	arg1	sp					1667:1668	Bacillus miscanthi sp	1648:1668	the name Bacillus miscanthi sp	1639:1668	The genotypic and phenotypic characteristics suggested that strain AK13T represented a novel species of the genus Bacillus, and thus the name Bacillus miscanthi sp.
31951191	7	79	theme	nucleotide	725:734	arg1	%					872:872	90.93, 91.05 and 71.87 %	849:872	%	872:872	The average nucleotide identity values between strain AK13T and B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T were 90.93, 91.05 and 71.87 %, respectively, with the digital DNA-DNA hybridization values of 42.7, 42.6 and 18.8 %, respectively.
31951191	7	79	theme	nucleotide	725:734	arg1	values					745:750	The average nucleotide identity values	713:750	The average nucleotide identity values between strain AK13T and B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T	713:842	The average nucleotide identity values between strain AK13T and B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T were 90.93, 91.05 and 71.87 %, respectively, with the digital DNA-DNA hybridization values of 42.7, 42.6 and 18.8 %, respectively.
31951191	5	80	theme	AK13T	448:452	arg1	%					480:480	40 mol%	474:480	40 mol%	474:480	The genomic DNA G+C content of strain AK13T was estimated to be 40 mol%.
31951191	5	80	theme	AK13T	448:452	arg1	content					430:436	The genomic DNA G+C content	410:436	The genomic DNA G+C content of strain AK13T	410:452	The genomic DNA G+C content of strain AK13T was estimated to be 40 mol%.
31951191	10	81	theme	analysis	1249:1256	arg1	Results					1214:1220	Results	1214:1220	Results of fatty acid methyl ester analysis	1214:1256	Results of fatty acid methyl ester analysis revealed that iso-C15 : 0 was the predominant cellular fatty acid.
31951191	2	82	theme	21401T=DSM	155:164	arg1	109981T					166:172	=KACC 21401T=DSM 109981T	149:172	=KACC 21401T=DSM 109981T	149:172	A novel bacterial strain, designated AK13T (=KACC 21401T=DSM 109981T), was isolated from the rhizosphere of Miscanthus sacchariflorus.
31951191	2	82	theme	21401T=DSM	155:164	arg1	AK13T					142:146	AK13T	142:146	AK13T (=KACC 21401T=DSM 109981T)	142:173	A novel bacterial strain, designated AK13T (=KACC 21401T=DSM 109981T), was isolated from the rhizosphere of Miscanthus sacchariflorus.
31951191	9	83	theme	AK13T	1129:1133	arg1	wall					1114:1117	The cell wall	1105:1117	The cell wall of strain AK13T	1105:1133	The cell wall of strain AK13T contained meso-diaminopimelic acid, and the major isoprenoid quinone was MK-7.
31951191	8	84	theme	5-40	989:992	arg1	pH					1018:1019	pH 6.5-13	1018:1026	pH 6.5-13 (optimum, pH 8-9)	1018:1044	Cells grew at 5-40 °C (optimum, 28-35 °C), pH 6.5-13 (optimum, pH 8-9) and in the presence of 0-13.0 % (w/v) NaCl (optimum, 1 %).
31951191	8	84	theme	5-40	989:992	arg1	optimum					998:1004	optimum	998:1004	optimum	998:1004	Cells grew at 5-40 °C (optimum, 28-35 °C), pH 6.5-13 (optimum, pH 8-9) and in the presence of 0-13.0 % (w/v) NaCl (optimum, 1 %).
31951191	8	84	theme	5-40	989:992	arg1	°C					994:995	5-40 °C	989:995	5-40 °C (optimum, 28-35 °C)	989:1015	Cells grew at 5-40 °C (optimum, 28-35 °C), pH 6.5-13 (optimum, pH 8-9) and in the presence of 0-13.0 % (w/v) NaCl (optimum, 1 %).
31951191	10	85	theme	fatty	1313:1317	arg1	iso-C15					1272:1278	iso-C15 : 0	1272:1282	iso-C15 : 0	1272:1282	Results of fatty acid methyl ester analysis revealed that iso-C15 : 0 was the predominant cellular fatty acid.
31951191	10	85	theme	fatty	1313:1317	arg1	acid					1319:1322	the predominant cellular fatty acid	1288:1322	the predominant cellular fatty acid	1288:1322	Results of fatty acid methyl ester analysis revealed that iso-C15 : 0 was the predominant cellular fatty acid.
31951191	7	86	theme	PAT	836:838	arg1	05T					840:842	B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T	777:842	B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T	777:842	The average nucleotide identity values between strain AK13T and B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T were 90.93, 91.05 and 71.87 %, respectively, with the digital DNA-DNA hybridization values of 42.7, 42.6 and 18.8 %, respectively.
31951191	9	87	theme	meso-diaminopimelic	1145:1163	arg1	acid					1165:1168	meso-diaminopimelic acid	1145:1168	meso-diaminopimelic acid	1145:1168	The cell wall of strain AK13T contained meso-diaminopimelic acid, and the major isoprenoid quinone was MK-7.
31951191	12	88	theme	Bacillus	1648:1655	arg1	sp					1667:1668	Bacillus miscanthi sp	1648:1668	the name Bacillus miscanthi sp	1639:1668	The genotypic and phenotypic characteristics suggested that strain AK13T represented a novel species of the genus Bacillus, and thus the name Bacillus miscanthi sp.
31951191	5	89	theme	strain	441:446	arg1	AK13T					448:452	strain AK13T	441:452	strain AK13T	441:452	The genomic DNA G+C content of strain AK13T was estimated to be 40 mol%.
31951191	6	90	dep	Bacillus	672:679	arg1	patagoniensis					681:693	patagoniensis	681:693	patagoniensis	681:693	Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that this strain was most closely related to Bacillus lehensis MLB2T (99.4 %), Bacillus oshimensis K11T (98.8 %) and Bacillus patagoniensis PAT 05T (96.6 %).
31951191	6	91	theme	gene	523:526	arg1	similarity					537:546	16S rRNA gene sequence similarity	514:546	16S rRNA gene sequence similarity	514:546	Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that this strain was most closely related to Bacillus lehensis MLB2T (99.4 %), Bacillus oshimensis K11T (98.8 %) and Bacillus patagoniensis PAT 05T (96.6 %).
31951191	11	92	theme	major	1395:1399	arg1	diphosphatidylglycerol					1419:1440	diphosphatidylglycerol	1419:1440	diphosphatidylglycerol	1419:1440	Two-dimensional thin-layer chromatography analysis indicated that the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and glycolipid.
31951191	11	92	theme	major	1395:1399	arg1	lipids					1407:1412	the major polar lipids	1391:1412	the major polar lipids	1391:1412	Two-dimensional thin-layer chromatography analysis indicated that the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and glycolipid.
31951191	7	93	theme	K11T	810:813	arg1	05T					840:842	B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T	777:842	B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T	777:842	The average nucleotide identity values between strain AK13T and B. lehensis MLB2T, B. oshimensis K11T and B. patagoniensis PAT 05T were 90.93, 91.05 and 71.87 %, respectively, with the digital DNA-DNA hybridization values of 42.7, 42.6 and 18.8 %, respectively.
31613202	11	0	theme	Streptomyces	1383:1394	arg1	sp					1405:1406	the name Streptomyces cavernae sp	1374:1406	the name Streptomyces cavernae sp	1374:1406	On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10008T represents a novel species of the genus Streptomyces, for which the name Streptomyces cavernae sp.
31613202	2	1	theme	novel	102:106	arg1	strain					124:129	A novel actinobacterial strain	100:129	A novel actinobacterial strain	100:129	A novel actinobacterial strain, designated SYSU K10008T, was isolated from a soil sample collected from a karst cave in Xingyi County, Guizhou Province, south-western PR China.
31613202	11	2	dep	data	1284:1287	arg1	basis					1236:1240	basis	1236:1240	basis	1236:1240	On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10008T represents a novel species of the genus Streptomyces, for which the name Streptomyces cavernae sp.
31613202	11	2	dep	data	1284:1287	arg1	the					1232:1234	the	1232:1234	the	1232:1234	On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10008T represents a novel species of the genus Streptomyces, for which the name Streptomyces cavernae sp.
31613202	3	3	theme	taxonomic	281:289	arg1	position					291:298	The taxonomic position	277:298	The taxonomic position of the strain	277:312	The taxonomic position of the strain was investigated by using a polyphasic approach.
31613202	11	4	theme	cavernae	1396:1403	arg1	sp					1405:1406	the name Streptomyces cavernae sp	1374:1406	the name Streptomyces cavernae sp	1374:1406	On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10008T represents a novel species of the genus Streptomyces, for which the name Streptomyces cavernae sp.
31613202	11	5	theme	SYSU	1297:1300	arg1	K10008T					1302:1308	strain SYSU K10008T	1290:1308	strain SYSU K10008T	1290:1308	On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10008T represents a novel species of the genus Streptomyces, for which the name Streptomyces cavernae sp.
31613202	8	6	theme	fatty	894:898	arg1	acids					900:904	the major fatty acids	884:904	the major fatty acids (>10 %)	884:912	The major isoprenoid quinone was MK-9(H6), while the major fatty acids (>10 %) were C16 : 0, iso-C16 : 0, anteiso-C15 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
31613202	8	6	theme	fatty	894:898	arg1	%					911:911	>10 %	907:911	>10 %	907:911	The major isoprenoid quinone was MK-9(H6), while the major fatty acids (>10 %) were C16 : 0, iso-C16 : 0, anteiso-C15 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
31613202	8	6	theme	fatty	894:898	arg1	C16 					919:922	C16 	919:922	C16 : 0, iso-C16 : 0, anteiso-C15 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c)	919:1002	The major isoprenoid quinone was MK-9(H6), while the major fatty acids (>10 %) were C16 : 0, iso-C16 : 0, anteiso-C15 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
31613202	8	7	theme	summed	961:966	arg1	feature					968:974	summed feature 3	961:976	summed feature 3	961:976	The major isoprenoid quinone was MK-9(H6), while the major fatty acids (>10 %) were C16 : 0, iso-C16 : 0, anteiso-C15 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
31613202	13	8	theme	type	1431:1434	arg1	strain					1436:1441	The type strain	1427:1441	The type strain	1427:1441	The type strain is SYSU K10008T (=KCTC 39850T=DSM 104115T).
31613202	13	8	theme	type	1431:1434	arg1	K10008T					1451:1457	K10008T	1451:1457	K10008T	1451:1457	The type strain is SYSU K10008T (=KCTC 39850T=DSM 104115T).
31613202	6	9	theme	diamino	745:751	arg1	acid					753:756	the diagnostic diamino acid	730:756	the diagnostic diamino acid in cell-wall peptidoglycan	730:783	In addition, ll-diaminopimelic acid was the diagnostic diamino acid in cell-wall peptidoglycan.
31613202	6	9	theme	diamino	745:751	arg1	acid					721:724	ll-diaminopimelic acid	703:724	ll-diaminopimelic acid	703:724	In addition, ll-diaminopimelic acid was the diagnostic diamino acid in cell-wall peptidoglycan.
31613202	8	10	dep	C16 	919:922	arg1	ω6c					999:1001	C16 : 1 ω7c/C16 : 1 ω6c	979:1001	C16 : 1 ω7c/C16 : 1 ω6c	979:1001	The major isoprenoid quinone was MK-9(H6), while the major fatty acids (>10 %) were C16 : 0, iso-C16 : 0, anteiso-C15 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
31613202	8	10	dep	C16 	919:922	arg1	 0					924:925	 0	924:925	 0	924:925	The major isoprenoid quinone was MK-9(H6), while the major fatty acids (>10 %) were C16 : 0, iso-C16 : 0, anteiso-C15 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
31613202	8	10	dep	C16 	919:922	arg1	feature					968:974	summed feature 3	961:976	summed feature 3	961:976	The major isoprenoid quinone was MK-9(H6), while the major fatty acids (>10 %) were C16 : 0, iso-C16 : 0, anteiso-C15 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
31613202	8	10	dep	C16 	919:922	arg1	 0					954:955	 0	954:955	 0	954:955	The major isoprenoid quinone was MK-9(H6), while the major fatty acids (>10 %) were C16 : 0, iso-C16 : 0, anteiso-C15 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
31613202	11	11	theme	strain	1290:1295	arg1	K10008T					1302:1308	strain SYSU K10008T	1290:1308	strain SYSU K10008T	1290:1308	On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10008T represents a novel species of the genus Streptomyces, for which the name Streptomyces cavernae sp.
31613202	5	12	theme	NCBI	675:678	arg1	database					680:687	the NCBI database	671:687	the NCBI database	671:687	On the basis of 16S rRNA gene sequence similarities and the results of phylogenetic analysis, strain SYSU K10008T was most closely related to Streptomyces cyaneus CGMCC 4.1671T, and shared the highest sequence identity of 98.3 % based on the NCBI database.
31613202	10	13	theme	DNA	1175:1177	arg1	content					1183:1189	The genomic DNA G+C content	1163:1189	The genomic DNA G+C content of strain SYSU K10008T	1163:1212	The genomic DNA G+C content of strain SYSU K10008T was 70.5 mol%.
31613202	10	13	theme	DNA	1175:1177	arg1	%					1226:1226	70.5 mol%	1218:1226	70.5 mol%	1218:1226	The genomic DNA G+C content of strain SYSU K10008T was 70.5 mol%.
31613202	6	14	theme	diagnostic	734:743	arg1	acid					753:756	the diagnostic diamino acid	730:756	the diagnostic diamino acid in cell-wall peptidoglycan	730:783	In addition, ll-diaminopimelic acid was the diagnostic diamino acid in cell-wall peptidoglycan.
31613202	6	14	theme	diagnostic	734:743	arg1	acid					721:724	ll-diaminopimelic acid	703:724	ll-diaminopimelic acid	703:724	In addition, ll-diaminopimelic acid was the diagnostic diamino acid in cell-wall peptidoglycan.
31613202	5	15	theme	16S	449:451	arg1	rRNA					453:456	16S rRNA	449:456	16S rRNA gene sequence similarities	449:483	On the basis of 16S rRNA gene sequence similarities and the results of phylogenetic analysis, strain SYSU K10008T was most closely related to Streptomyces cyaneus CGMCC 4.1671T, and shared the highest sequence identity of 98.3 % based on the NCBI database.
31613202	3	16	theme	strain	307:312	arg1	position					291:298	The taxonomic position	277:298	The taxonomic position of the strain	277:312	The taxonomic position of the strain was investigated by using a polyphasic approach.
31613202	5	17	theme	Streptomyces	575:586	arg1	4.1671T					602:608	Streptomyces cyaneus CGMCC 4.1671T	575:608	Streptomyces cyaneus CGMCC 4.1671T	575:608	On the basis of 16S rRNA gene sequence similarities and the results of phylogenetic analysis, strain SYSU K10008T was most closely related to Streptomyces cyaneus CGMCC 4.1671T, and shared the highest sequence identity of 98.3 % based on the NCBI database.
31613202	0	18	theme	Streptomyces	0:11	arg1	sp					22:23	Streptomyces cavernae sp	0:23	Streptomyces cavernae sp.	0:24	Streptomyces cavernae sp.
31613202	5	19	theme	rRNA	453:456	arg1	similarities					472:483	16S rRNA gene sequence similarities	449:483	16S rRNA gene sequence similarities	449:483	On the basis of 16S rRNA gene sequence similarities and the results of phylogenetic analysis, strain SYSU K10008T was most closely related to Streptomyces cyaneus CGMCC 4.1671T, and shared the highest sequence identity of 98.3 % based on the NCBI database.
31613202	8	20	dep	 0	924:925	arg1	 0					937:938	 0	937:938	 0	937:938	The major isoprenoid quinone was MK-9(H6), while the major fatty acids (>10 %) were C16 : 0, iso-C16 : 0, anteiso-C15 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
31613202	0	21	theme	cavernae	13:20	arg1	sp					22:23	Streptomyces cavernae sp	0:23	Streptomyces cavernae sp.	0:24	Streptomyces cavernae sp.
31613202	8	22	theme	ω7c/C16	987:993	arg1	ω6c					999:1001	C16 : 1 ω7c/C16 : 1 ω6c	979:1001	C16 : 1 ω7c/C16 : 1 ω6c	979:1001	The major isoprenoid quinone was MK-9(H6), while the major fatty acids (>10 %) were C16 : 0, iso-C16 : 0, anteiso-C15 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
31613202	8	22	theme	ω7c/C16	987:993	arg1	 0					954:955	 0	954:955	 0	954:955	The major isoprenoid quinone was MK-9(H6), while the major fatty acids (>10 %) were C16 : 0, iso-C16 : 0, anteiso-C15 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
31613202	1	23	theme	karst	72:76	arg1	sample					92:97	a karst cave sediment sample	70:97	a karst cave sediment sample	70:97	nov., a novel actinobacterium isolated from a karst cave sediment sample.
31613202	5	24	theme	gene	458:461	arg1	similarities					472:483	16S rRNA gene sequence similarities	449:483	16S rRNA gene sequence similarities	449:483	On the basis of 16S rRNA gene sequence similarities and the results of phylogenetic analysis, strain SYSU K10008T was most closely related to Streptomyces cyaneus CGMCC 4.1671T, and shared the highest sequence identity of 98.3 % based on the NCBI database.
31613202	3	25	theme	polyphasic	342:351	arg1	approach					353:360	a polyphasic approach	340:360	a polyphasic approach	340:360	The taxonomic position of the strain was investigated by using a polyphasic approach.
31613202	9	26	theme	polar	1009:1013	arg1	lipids					1015:1020	The polar lipids	1005:1020	The polar lipids	1005:1020	The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and one unidentified lipid.
31613202	5	27	theme	CGMCC	596:600	arg1	4.1671T					602:608	Streptomyces cyaneus CGMCC 4.1671T	575:608	Streptomyces cyaneus CGMCC 4.1671T	575:608	On the basis of 16S rRNA gene sequence similarities and the results of phylogenetic analysis, strain SYSU K10008T was most closely related to Streptomyces cyaneus CGMCC 4.1671T, and shared the highest sequence identity of 98.3 % based on the NCBI database.
31613202	1	28	theme	cave	78:81	arg1	sample					92:97	a karst cave sediment sample	70:97	a karst cave sediment sample	70:97	nov., a novel actinobacterium isolated from a karst cave sediment sample.
31613202	8	29	theme	isoprenoid	845:854	arg1	MK-9					868:871	MK-9	868:871	MK-9	868:871	The major isoprenoid quinone was MK-9(H6), while the major fatty acids (>10 %) were C16 : 0, iso-C16 : 0, anteiso-C15 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
31613202	8	29	theme	isoprenoid	845:854	arg1	quinone					856:862	The major isoprenoid quinone	835:862	The major isoprenoid quinone	835:862	The major isoprenoid quinone was MK-9(H6), while the major fatty acids (>10 %) were C16 : 0, iso-C16 : 0, anteiso-C15 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
31613202	6	30	from	acid	753:756	arg1	addition					693:700	addition	693:700	addition	693:700	In addition, ll-diaminopimelic acid was the diagnostic diamino acid in cell-wall peptidoglycan.
31613202	6	30	from	acid	753:756	arg1	peptidoglycan					771:783	cell-wall peptidoglycan	761:783	cell-wall peptidoglycan	761:783	In addition, ll-diaminopimelic acid was the diagnostic diamino acid in cell-wall peptidoglycan.
31613202	6	31	theme	ll-diaminopimelic	703:719	arg1	acid					753:756	the diagnostic diamino acid	730:756	the diagnostic diamino acid in cell-wall peptidoglycan	730:783	In addition, ll-diaminopimelic acid was the diagnostic diamino acid in cell-wall peptidoglycan.
31613202	6	31	theme	ll-diaminopimelic	703:719	arg1	acid					721:724	ll-diaminopimelic acid	703:724	ll-diaminopimelic acid	703:724	In addition, ll-diaminopimelic acid was the diagnostic diamino acid in cell-wall peptidoglycan.
31613202	1	32	theme	sediment	83:90	arg1	sample					92:97	a karst cave sediment sample	70:97	a karst cave sediment sample	70:97	nov., a novel actinobacterium isolated from a karst cave sediment sample.
31613202	2	33	from	cave	212:215	arg1	Province					243:250	Province	243:250	Province	243:250	A novel actinobacterial strain, designated SYSU K10008T, was isolated from a soil sample collected from a karst cave in Xingyi County, Guizhou Province, south-western PR China.
31613202	2	33	from	cave	212:215	arg1	China					270:274	China	270:274	China	270:274	A novel actinobacterial strain, designated SYSU K10008T, was isolated from a soil sample collected from a karst cave in Xingyi County, Guizhou Province, south-western PR China.
31613202	2	33	from	cave	212:215	arg1	County					227:232	County	227:232	County	227:232	A novel actinobacterial strain, designated SYSU K10008T, was isolated from a soil sample collected from a karst cave in Xingyi County, Guizhou Province, south-western PR China.
31613202	10	34	theme	K10008T	1206:1212	arg1	content					1183:1189	The genomic DNA G+C content	1163:1189	The genomic DNA G+C content of strain SYSU K10008T	1163:1212	The genomic DNA G+C content of strain SYSU K10008T was 70.5 mol%.
31613202	10	34	theme	K10008T	1206:1212	arg1	%					1226:1226	70.5 mol%	1218:1226	70.5 mol%	1218:1226	The genomic DNA G+C content of strain SYSU K10008T was 70.5 mol%.
31613202	13	35	theme	39850T=DSM	1466:1475	arg1	104115T					1477:1483	=KCTC 39850T=DSM 104115T	1460:1483	=KCTC 39850T=DSM 104115T	1460:1483	The type strain is SYSU K10008T (=KCTC 39850T=DSM 104115T).
31613202	13	35	theme	39850T=DSM	1466:1475	arg1	K10008T					1451:1457	K10008T	1451:1457	K10008T	1451:1457	The type strain is SYSU K10008T (=KCTC 39850T=DSM 104115T).
31613202	10	36	theme	G+C	1179:1181	arg1	content					1183:1189	The genomic DNA G+C content	1163:1189	The genomic DNA G+C content of strain SYSU K10008T	1163:1212	The genomic DNA G+C content of strain SYSU K10008T was 70.5 mol%.
31613202	10	36	theme	G+C	1179:1181	arg1	%					1226:1226	70.5 mol%	1218:1226	70.5 mol%	1218:1226	The genomic DNA G+C content of strain SYSU K10008T was 70.5 mol%.
31613202	2	37	attach	isolated	161:168	arg2	strain					124:129	A novel actinobacterial strain	100:129	A novel actinobacterial strain	100:129	A novel actinobacterial strain, designated SYSU K10008T, was isolated from a soil sample collected from a karst cave in Xingyi County, Guizhou Province, south-western PR China.
31613202	2	37	attach	isolated	161:168	arg1	sample					182:187	a soil sample	175:187	a soil sample collected from a karst cave in Xingyi County, Guizhou Province, south-western PR China	175:274	A novel actinobacterial strain, designated SYSU K10008T, was isolated from a soil sample collected from a karst cave in Xingyi County, Guizhou Province, south-western PR China.
31613202	10	38	theme	genomic	1167:1173	arg1	content					1183:1189	The genomic DNA G+C content	1163:1189	The genomic DNA G+C content of strain SYSU K10008T	1163:1212	The genomic DNA G+C content of strain SYSU K10008T was 70.5 mol%.
31613202	10	38	theme	genomic	1167:1173	arg1	%					1226:1226	70.5 mol%	1218:1226	70.5 mol%	1218:1226	The genomic DNA G+C content of strain SYSU K10008T was 70.5 mol%.
31613202	5	39	theme	%	660:660	arg1	identity					643:650	the highest sequence identity	622:650	the highest sequence identity of 98.3 %	622:660	On the basis of 16S rRNA gene sequence similarities and the results of phylogenetic analysis, strain SYSU K10008T was most closely related to Streptomyces cyaneus CGMCC 4.1671T, and shared the highest sequence identity of 98.3 % based on the NCBI database.
31613202	2	40	theme	karst	206:210	arg1	cave					212:215	a karst cave	204:215	a karst cave in Xingyi County, Guizhou Province, south-western PR China	204:274	A novel actinobacterial strain, designated SYSU K10008T, was isolated from a soil sample collected from a karst cave in Xingyi County, Guizhou Province, south-western PR China.
31613202	5	41	dep	similarities	472:483	arg1	the					436:438	the	436:438	the	436:438	On the basis of 16S rRNA gene sequence similarities and the results of phylogenetic analysis, strain SYSU K10008T was most closely related to Streptomyces cyaneus CGMCC 4.1671T, and shared the highest sequence identity of 98.3 % based on the NCBI database.
31613202	5	41	dep	similarities	472:483	arg1	basis					440:444	basis	440:444	basis	440:444	On the basis of 16S rRNA gene sequence similarities and the results of phylogenetic analysis, strain SYSU K10008T was most closely related to Streptomyces cyaneus CGMCC 4.1671T, and shared the highest sequence identity of 98.3 % based on the NCBI database.
31613202	9	42	theme	phosphatidylinositol	1104:1123	arg1	mannoside					1125:1133	phosphatidylinositol mannoside	1104:1133	phosphatidylinositol mannoside	1104:1133	The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and one unidentified lipid.
31613202	5	43	theme	results	493:499	arg1	related					564:570	related	564:570	related	564:570	On the basis of 16S rRNA gene sequence similarities and the results of phylogenetic analysis, strain SYSU K10008T was most closely related to Streptomyces cyaneus CGMCC 4.1671T, and shared the highest sequence identity of 98.3 % based on the NCBI database.
31613202	11	44	theme	phylogenetic	1271:1282	arg1	data					1284:1287	phenotypic, genotypic and phylogenetic data	1245:1287	phenotypic, genotypic and phylogenetic data	1245:1287	On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10008T represents a novel species of the genus Streptomyces, for which the name Streptomyces cavernae sp.
31613202	13	45	theme	=KCTC	1460:1464	arg1	104115T					1477:1483	=KCTC 39850T=DSM 104115T	1460:1483	=KCTC 39850T=DSM 104115T	1460:1483	The type strain is SYSU K10008T (=KCTC 39850T=DSM 104115T).
31613202	13	45	theme	=KCTC	1460:1464	arg1	K10008T					1451:1457	K10008T	1451:1457	K10008T	1451:1457	The type strain is SYSU K10008T (=KCTC 39850T=DSM 104115T).
31613202	8	46	theme	C16 	979:982	arg1	ω6c					999:1001	C16 : 1 ω7c/C16 : 1 ω6c	979:1001	C16 : 1 ω7c/C16 : 1 ω6c	979:1001	The major isoprenoid quinone was MK-9(H6), while the major fatty acids (>10 %) were C16 : 0, iso-C16 : 0, anteiso-C15 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
31613202	8	46	theme	C16 	979:982	arg1	 0					954:955	 0	954:955	 0	954:955	The major isoprenoid quinone was MK-9(H6), while the major fatty acids (>10 %) were C16 : 0, iso-C16 : 0, anteiso-C15 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
31613202	5	47	dep	Streptomyces	575:586	arg1	cyaneus					588:594	cyaneus	588:594	cyaneus	588:594	On the basis of 16S rRNA gene sequence similarities and the results of phylogenetic analysis, strain SYSU K10008T was most closely related to Streptomyces cyaneus CGMCC 4.1671T, and shared the highest sequence identity of 98.3 % based on the NCBI database.
31613202	5	48	theme	highest	626:632	arg1	identity					643:650	the highest sequence identity	622:650	the highest sequence identity of 98.3 %	622:660	On the basis of 16S rRNA gene sequence similarities and the results of phylogenetic analysis, strain SYSU K10008T was most closely related to Streptomyces cyaneus CGMCC 4.1671T, and shared the highest sequence identity of 98.3 % based on the NCBI database.
31613202	4	49	theme	strain	376:381	arg1	Cells					363:367	Cells	363:367	Cells of the strain	363:381	Cells of the strain were aerobic, Gram-stain-positive and non-motile.
31613202	5	50	theme	phylogenetic	504:515	arg1	analysis					517:524	phylogenetic analysis	504:524	phylogenetic analysis	504:524	On the basis of 16S rRNA gene sequence similarities and the results of phylogenetic analysis, strain SYSU K10008T was most closely related to Streptomyces cyaneus CGMCC 4.1671T, and shared the highest sequence identity of 98.3 % based on the NCBI database.
31613202	11	51	theme	genus	1344:1348	arg1	Streptomyces					1350:1361	the genus Streptomyces	1340:1361	the genus Streptomyces	1340:1361	On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10008T represents a novel species of the genus Streptomyces, for which the name Streptomyces cavernae sp.
31613202	5	52	theme	sequence	634:641	arg1	identity					643:650	the highest sequence identity	622:650	the highest sequence identity of 98.3 %	622:660	On the basis of 16S rRNA gene sequence similarities and the results of phylogenetic analysis, strain SYSU K10008T was most closely related to Streptomyces cyaneus CGMCC 4.1671T, and shared the highest sequence identity of 98.3 % based on the NCBI database.
31613202	2	53	theme	soil	177:180	arg1	sample					182:187	a soil sample	175:187	a soil sample collected from a karst cave in Xingyi County, Guizhou Province, south-western PR China	175:274	A novel actinobacterial strain, designated SYSU K10008T, was isolated from a soil sample collected from a karst cave in Xingyi County, Guizhou Province, south-western PR China.
31613202	11	54	theme	name	1378:1381	arg1	sp					1405:1406	the name Streptomyces cavernae sp	1374:1406	the name Streptomyces cavernae sp	1374:1406	On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10008T represents a novel species of the genus Streptomyces, for which the name Streptomyces cavernae sp.
31613202	5	55	theme	analysis	517:524	arg1	similarities					472:483	16S rRNA gene sequence similarities	449:483	16S rRNA gene sequence similarities	449:483	On the basis of 16S rRNA gene sequence similarities and the results of phylogenetic analysis, strain SYSU K10008T was most closely related to Streptomyces cyaneus CGMCC 4.1671T, and shared the highest sequence identity of 98.3 % based on the NCBI database.
31613202	5	55	theme	analysis	517:524	arg1	results					493:499	the results	489:499	the results of phylogenetic analysis	489:524	On the basis of 16S rRNA gene sequence similarities and the results of phylogenetic analysis, strain SYSU K10008T was most closely related to Streptomyces cyaneus CGMCC 4.1671T, and shared the highest sequence identity of 98.3 % based on the NCBI database.
31613202	10	56	theme	strain	1194:1199	arg1	K10008T					1206:1212	strain SYSU K10008T	1194:1212	strain SYSU K10008T	1194:1212	The genomic DNA G+C content of strain SYSU K10008T was 70.5 mol%.
31613202	11	57	theme	Streptomyces	1350:1361	arg1	species					1329:1335	a novel species	1321:1335	a novel species	1321:1335	On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10008T represents a novel species of the genus Streptomyces, for which the name Streptomyces cavernae sp.
31613202	9	58	contain	contained	1022:1030	arg1	lipids					1015:1020	The polar lipids	1005:1020	The polar lipids	1005:1020	The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and one unidentified lipid.
31613202	9	58	contain	contained	1022:1030	arg2	phosphatidylethanolamine					1056:1079	phosphatidylethanolamine	1056:1079	phosphatidylethanolamine	1056:1079	The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and one unidentified lipid.
31613202	9	58	contain	contained	1022:1030	arg2	lipid					1156:1160	one unidentified lipid	1139:1160	one unidentified lipid	1139:1160	The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and one unidentified lipid.
31613202	9	58	contain	contained	1022:1030	arg2	phosphatidylinositol					1082:1101	phosphatidylinositol	1082:1101	phosphatidylinositol	1082:1101	The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and one unidentified lipid.
31613202	9	58	contain	contained	1022:1030	arg2	mannoside					1125:1133	phosphatidylinositol mannoside	1104:1133	phosphatidylinositol mannoside	1104:1133	The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and one unidentified lipid.
31613202	9	58	contain	contained	1022:1030	arg2	diphosphatidylglycerol					1032:1053	diphosphatidylglycerol	1032:1053	diphosphatidylglycerol	1032:1053	The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and one unidentified lipid.
31613202	10	59	theme	SYSU	1201:1204	arg1	K10008T					1206:1212	strain SYSU K10008T	1194:1212	strain SYSU K10008T	1194:1212	The genomic DNA G+C content of strain SYSU K10008T was 70.5 mol%.
31613202	9	60	theme	unidentified	1143:1154	arg1	lipid					1156:1160	one unidentified lipid	1139:1160	one unidentified lipid	1139:1160	The polar lipids contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and one unidentified lipid.
31613202	10	61	theme	70.5 mol	1218:1225	arg1	content					1183:1189	The genomic DNA G+C content	1163:1189	The genomic DNA G+C content of strain SYSU K10008T	1163:1212	The genomic DNA G+C content of strain SYSU K10008T was 70.5 mol%.
31613202	10	61	theme	70.5 mol	1218:1225	arg1	%					1226:1226	70.5 mol%	1218:1226	70.5 mol%	1218:1226	The genomic DNA G+C content of strain SYSU K10008T was 70.5 mol%.
31613202	5	62	theme	sequence	463:470	arg1	similarities					472:483	16S rRNA gene sequence similarities	449:483	16S rRNA gene sequence similarities	449:483	On the basis of 16S rRNA gene sequence similarities and the results of phylogenetic analysis, strain SYSU K10008T was most closely related to Streptomyces cyaneus CGMCC 4.1671T, and shared the highest sequence identity of 98.3 % based on the NCBI database.
31613202	8	63	theme	major	839:843	arg1	MK-9					868:871	MK-9	868:871	MK-9	868:871	The major isoprenoid quinone was MK-9(H6), while the major fatty acids (>10 %) were C16 : 0, iso-C16 : 0, anteiso-C15 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
31613202	8	63	theme	major	839:843	arg1	quinone					856:862	The major isoprenoid quinone	835:862	The major isoprenoid quinone	835:862	The major isoprenoid quinone was MK-9(H6), while the major fatty acids (>10 %) were C16 : 0, iso-C16 : 0, anteiso-C15 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
31613202	7	64	theme	whole-cell	790:799	arg1	sugars					801:806	The whole-cell sugars	786:806	The whole-cell sugars	786:806	The whole-cell sugars were glucose and rhamnose.
31613202	7	64	theme	whole-cell	790:799	arg1	glucose					813:819	glucose	813:819	glucose	813:819	The whole-cell sugars were glucose and rhamnose.
31613202	11	65	theme	phenotypic	1245:1254	arg1	data					1284:1287	phenotypic, genotypic and phylogenetic data	1245:1287	phenotypic, genotypic and phylogenetic data	1245:1287	On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10008T represents a novel species of the genus Streptomyces, for which the name Streptomyces cavernae sp.
31613202	6	66	theme	cell-wall	761:769	arg1	peptidoglycan					771:783	cell-wall peptidoglycan	761:783	cell-wall peptidoglycan	761:783	In addition, ll-diaminopimelic acid was the diagnostic diamino acid in cell-wall peptidoglycan.
31613202	5	67	theme	similarities	472:483	arg1	related					564:570	related	564:570	related	564:570	On the basis of 16S rRNA gene sequence similarities and the results of phylogenetic analysis, strain SYSU K10008T was most closely related to Streptomyces cyaneus CGMCC 4.1671T, and shared the highest sequence identity of 98.3 % based on the NCBI database.
31613202	11	68	theme	novel	1323:1327	arg1	species					1329:1335	a novel species	1321:1335	a novel species	1321:1335	On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10008T represents a novel species of the genus Streptomyces, for which the name Streptomyces cavernae sp.
31613202	2	69	theme	actinobacterial	108:122	arg1	strain					124:129	A novel actinobacterial strain	100:129	A novel actinobacterial strain	100:129	A novel actinobacterial strain, designated SYSU K10008T, was isolated from a soil sample collected from a karst cave in Xingyi County, Guizhou Province, south-western PR China.
31613202	1	70	theme	novel	34:38	arg1	actinobacterium					40:54	a novel actinobacterium	32:54	a novel actinobacterium	32:54	nov., a novel actinobacterium isolated from a karst cave sediment sample.
31613202	1	70	theme	novel	34:38	arg1	nov.					26:29	nov.	26:29	nov.	26:29	nov., a novel actinobacterium isolated from a karst cave sediment sample.
31613202	8	71	theme	major	888:892	arg1	acids					900:904	the major fatty acids	884:904	the major fatty acids (>10 %)	884:912	The major isoprenoid quinone was MK-9(H6), while the major fatty acids (>10 %) were C16 : 0, iso-C16 : 0, anteiso-C15 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
31613202	8	71	theme	major	888:892	arg1	%					911:911	>10 %	907:911	>10 %	907:911	The major isoprenoid quinone was MK-9(H6), while the major fatty acids (>10 %) were C16 : 0, iso-C16 : 0, anteiso-C15 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
31613202	8	71	theme	major	888:892	arg1	C16 					919:922	C16 	919:922	C16 : 0, iso-C16 : 0, anteiso-C15 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c)	919:1002	The major isoprenoid quinone was MK-9(H6), while the major fatty acids (>10 %) were C16 : 0, iso-C16 : 0, anteiso-C15 : 0 and summed feature 3 (C16 : 1 ω7c/C16 : 1 ω6c).
31613202	11	72	theme	genotypic	1257:1265	arg1	data					1284:1287	phenotypic, genotypic and phylogenetic data	1245:1287	phenotypic, genotypic and phylogenetic data	1245:1287	On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10008T represents a novel species of the genus Streptomyces, for which the name Streptomyces cavernae sp.
32388252	11	0	theme	catch-up	1736:1743	arg1	growth					1745:1750	catch-up growth	1736:1750	catch-up growth	1736:1750	Thus, gut dysbiosis and chronic endotoxemia observed in U rats, even before catch-up growth, might anticipate a pro-inflammatory milieu promoting metabolic diseases when fed hyperlipidic diets.
32388252	5	1	from	ratio	885:889	arg1	pups					992:995	U pups	990:995	U pups	990:995	Analyses of gut microbial composition before catch-up growth revealed reduced F/B ratio and significant expansion of the mucolytic genera Akkermansia (P<.05) and Desulfovibrio (P<.05) in U pups.
32388252	1	2	theme	metabolic	250:258	arg1	syndrome					260:267	metabolic syndrome	250:267	metabolic syndrome	250:267	Nutritional restriction early in life followed by catch-up growth has been associated with increased risk of metabolic syndrome in adulthood.
32388252	9	3	theme	increased	1458:1466	arg1	levels					1480:1485	increased circulating levels	1458:1485	increased circulating levels of bacterial lipopolysaccharides in both U and UHF adult rats	1458:1547	This event was correlated with increased circulating levels of bacterial lipopolysaccharides in both U and UHF adult rats.
32388252	7	4	theme	U	1334:1334	arg1	rats					1342:1345	U adult rats	1334:1345	U adult rats	1334:1345	Food-restriction increased intestinal permeability causing disorganization on the tight-junction proteins of colonic epithelium, Zonula Occludens-1 (ZO-1) and occludin, and reducing the mucus thickness layer in U adult rats.
32388252	4	5	theme	UHF	777:779	arg1	rats					781:784	UHF rats	777:784	UHF rats compared to CHF	777:800	HF-feeding induced glucose intolerance (P<.05), insulin resistance (P<.001), and white adipose tissue inflammation (P<.001) in UHF rats compared to CHF.
32388252	6	6	theme	U	1075:1075	arg1	rats					1077:1080	U rats	1075:1080	U rats	1075:1080	Although relative abundance of Akkermansia remained elevated to adulthood in U rats, HFD normalized its levels to C and CHF.
32388252	2	7	theme	gut	312:314	arg1	colonization					316:327	altered gut colonization	304:327	altered gut colonization	304:327	To elucidate whether altered gut colonization underlies the mechanisms responsible of this predisposition gut microbiome was studied before or afterwards catch-up growth.
32388252	7	8	theme	mucus	1309:1313	arg1	layer					1325:1329	the mucus thickness layer	1305:1329	the mucus thickness layer in U adult rats	1305:1345	Food-restriction increased intestinal permeability causing disorganization on the tight-junction proteins of colonic epithelium, Zonula Occludens-1 (ZO-1) and occludin, and reducing the mucus thickness layer in U adult rats.
32388252	0	9	theme	catch-up	124:131	arg1	growth					133:138	nutrient-induced catch-up growth	107:138	nutrient-induced catch-up growth	107:138	Gut DYSBIOSIS and altered barrier function precedes the appearance of metabolic syndrome in a rat model of nutrient-induced catch-up growth.
32388252	5	10	theme	U	990:990	arg1	pups					992:995	U pups	990:995	U pups	990:995	Analyses of gut microbial composition before catch-up growth revealed reduced F/B ratio and significant expansion of the mucolytic genera Akkermansia (P<.05) and Desulfovibrio (P<.05) in U pups.
32388252	0	11	from	appearance	56:65	arg1	model					98:102	a rat model	92:102	a rat model of nutrient-induced catch-up growth	92:138	Gut DYSBIOSIS and altered barrier function precedes the appearance of metabolic syndrome in a rat model of nutrient-induced catch-up growth.
32388252	11	12	theme	chronic	1684:1690	arg1	endotoxemia					1692:1702	chronic endotoxemia	1684:1702	chronic endotoxemia	1684:1702	Thus, gut dysbiosis and chronic endotoxemia observed in U rats, even before catch-up growth, might anticipate a pro-inflammatory milieu promoting metabolic diseases when fed hyperlipidic diets.
32388252	1	13	from	risk	242:245	arg1	adulthood					272:280	adulthood	272:280	adulthood	272:280	Nutritional restriction early in life followed by catch-up growth has been associated with increased risk of metabolic syndrome in adulthood.
32388252	5	14	theme	composition	829:839	arg1	Analyses					803:810	Analyses	803:810	Analyses of gut microbial composition before catch-up growth	803:862	Analyses of gut microbial composition before catch-up growth revealed reduced F/B ratio and significant expansion of the mucolytic genera Akkermansia (P<.05) and Desulfovibrio (P<.05) in U pups.
32388252	9	15	from	levels	1480:1485	arg1	rats					1544:1547	both U and UHF adult rats	1523:1547	both U and UHF adult rats	1523:1547	This event was correlated with increased circulating levels of bacterial lipopolysaccharides in both U and UHF adult rats.
32388252	5	16	theme	catch-up	848:855	arg1	growth					857:862	catch-up growth	848:862	catch-up growth	848:862	Analyses of gut microbial composition before catch-up growth revealed reduced F/B ratio and significant expansion of the mucolytic genera Akkermansia (P<.05) and Desulfovibrio (P<.05) in U pups.
32388252	7	17	from	layer	1325:1329	arg1	rats					1342:1345	U adult rats	1334:1345	U adult rats	1334:1345	Food-restriction increased intestinal permeability causing disorganization on the tight-junction proteins of colonic epithelium, Zonula Occludens-1 (ZO-1) and occludin, and reducing the mucus thickness layer in U adult rats.
32388252	1	18	theme	Nutritional	141:151	arg1	restriction					153:163	Nutritional restriction	141:163	Nutritional restriction early in life followed by catch-up growth	141:205	Nutritional restriction early in life followed by catch-up growth has been associated with increased risk of metabolic syndrome in adulthood.
32388252	0	19	theme	metabolic	70:78	arg1	syndrome					80:87	metabolic syndrome	70:87	metabolic syndrome	70:87	Gut DYSBIOSIS and altered barrier function precedes the appearance of metabolic syndrome in a rat model of nutrient-induced catch-up growth.
32388252	1	20	from	restriction	153:163	arg1	life					174:177	life	174:177	life	174:177	Nutritional restriction early in life followed by catch-up growth has been associated with increased risk of metabolic syndrome in adulthood.
32388252	5	21	theme	gut	815:817	arg1	composition					829:839	gut microbial composition	815:839	gut microbial composition before catch-up growth	815:862	Analyses of gut microbial composition before catch-up growth revealed reduced F/B ratio and significant expansion of the mucolytic genera Akkermansia (P<.05) and Desulfovibrio (P<.05) in U pups.
32388252	8	22	theme	U	1402:1402	arg1	rats					1404:1407	U rats	1402:1407	U rats	1402:1407	The levels of ZO-1 and occludin were not recovered in U rats after HF-feeding.
32388252	0	23	theme	rat	94:96	arg1	model					98:102	a rat model	92:102	a rat model of nutrient-induced catch-up growth	92:138	Gut DYSBIOSIS and altered barrier function precedes the appearance of metabolic syndrome in a rat model of nutrient-induced catch-up growth.
32388252	0	24	theme	Gut	0:2	arg1	DYSBIOSIS					4:12	Gut DYSBIOSIS	0:12	Gut DYSBIOSIS	0:12	Gut DYSBIOSIS and altered barrier function precedes the appearance of metabolic syndrome in a rat model of nutrient-induced catch-up growth.
32388252	9	25	theme	circulating	1468:1478	arg1	levels					1480:1485	increased circulating levels	1458:1485	increased circulating levels of bacterial lipopolysaccharides in both U and UHF adult rats	1458:1547	This event was correlated with increased circulating levels of bacterial lipopolysaccharides in both U and UHF adult rats.
32388252	7	26	theme	Zonula	1252:1257	arg1	ZO-1					1272:1275	ZO-1	1272:1275	ZO-1	1272:1275	Food-restriction increased intestinal permeability causing disorganization on the tight-junction proteins of colonic epithelium, Zonula Occludens-1 (ZO-1) and occludin, and reducing the mucus thickness layer in U adult rats.
32388252	7	26	theme	Zonula	1252:1257	arg1	Occludens-1					1259:1269	Zonula Occludens-1	1252:1269	Zonula Occludens-1 (ZO-1)	1252:1276	Food-restriction increased intestinal permeability causing disorganization on the tight-junction proteins of colonic epithelium, Zonula Occludens-1 (ZO-1) and occludin, and reducing the mucus thickness layer in U adult rats.
32388252	7	26	theme	Zonula	1252:1257	arg1	proteins					1220:1227	the tight-junction proteins	1201:1227	the tight-junction proteins of colonic epithelium	1201:1249	Food-restriction increased intestinal permeability causing disorganization on the tight-junction proteins of colonic epithelium, Zonula Occludens-1 (ZO-1) and occludin, and reducing the mucus thickness layer in U adult rats.
32388252	5	27	theme	Akkermansia	941:951	arg1	ratio					885:889	reduced F/B ratio	873:889	reduced F/B ratio	873:889	Analyses of gut microbial composition before catch-up growth revealed reduced F/B ratio and significant expansion of the mucolytic genera Akkermansia (P<.05) and Desulfovibrio (P<.05) in U pups.
32388252	5	27	theme	Akkermansia	941:951	arg1	expansion					907:915	significant expansion	895:915	significant expansion	895:915	Analyses of gut microbial composition before catch-up growth revealed reduced F/B ratio and significant expansion of the mucolytic genera Akkermansia (P<.05) and Desulfovibrio (P<.05) in U pups.
32388252	11	28	theme	metabolic	1806:1814	arg1	diseases					1816:1823	metabolic diseases	1806:1823	metabolic diseases	1806:1823	Thus, gut dysbiosis and chronic endotoxemia observed in U rats, even before catch-up growth, might anticipate a pro-inflammatory milieu promoting metabolic diseases when fed hyperlipidic diets.
32388252	5	29	theme	F/B	881:883	arg1	ratio					885:889	reduced F/B ratio	873:889	reduced F/B ratio	873:889	Analyses of gut microbial composition before catch-up growth revealed reduced F/B ratio and significant expansion of the mucolytic genera Akkermansia (P<.05) and Desulfovibrio (P<.05) in U pups.
32388252	7	30	theme	epithelium	1240:1249	arg1	proteins					1220:1227	the tight-junction proteins	1201:1227	the tight-junction proteins of colonic epithelium	1201:1249	Food-restriction increased intestinal permeability causing disorganization on the tight-junction proteins of colonic epithelium, Zonula Occludens-1 (ZO-1) and occludin, and reducing the mucus thickness layer in U adult rats.
32388252	7	30	theme	epithelium	1240:1249	arg1	occludin					1282:1289	occludin	1282:1289	occludin	1282:1289	Food-restriction increased intestinal permeability causing disorganization on the tight-junction proteins of colonic epithelium, Zonula Occludens-1 (ZO-1) and occludin, and reducing the mucus thickness layer in U adult rats.
32388252	7	30	theme	epithelium	1240:1249	arg1	Occludens-1					1259:1269	Zonula Occludens-1	1252:1269	Zonula Occludens-1 (ZO-1)	1252:1276	Food-restriction increased intestinal permeability causing disorganization on the tight-junction proteins of colonic epithelium, Zonula Occludens-1 (ZO-1) and occludin, and reducing the mucus thickness layer in U adult rats.
32388252	10	31	theme	serum	1561:1565	arg1	lipopolysaccharides					1567:1585	serum lipopolysaccharides	1561:1585	serum lipopolysaccharides	1561:1585	Even more, serum lipopolysaccharides were already elevated in U rats compared to C group (P<.001) at weaning.
32388252	0	32	theme	barrier	26:32	arg1	function					34:41	altered barrier function	18:41	altered barrier function	18:41	Gut DYSBIOSIS and altered barrier function precedes the appearance of metabolic syndrome in a rat model of nutrient-induced catch-up growth.
32388252	9	33	theme	lipopolysaccharides	1500:1518	arg1	levels					1480:1485	increased circulating levels	1458:1485	increased circulating levels of bacterial lipopolysaccharides in both U and UHF adult rats	1458:1547	This event was correlated with increased circulating levels of bacterial lipopolysaccharides in both U and UHF adult rats.
32388252	10	34	theme	U	1612:1612	arg1	rats					1614:1617	U rats	1612:1617	U rats compared to C group (P<.001) at weaning	1612:1657	Even more, serum lipopolysaccharides were already elevated in U rats compared to C group (P<.001) at weaning.
32388252	7	35	theme	tight-junction	1205:1218	arg1	proteins					1220:1227	the tight-junction proteins	1201:1227	the tight-junction proteins of colonic epithelium	1201:1249	Food-restriction increased intestinal permeability causing disorganization on the tight-junction proteins of colonic epithelium, Zonula Occludens-1 (ZO-1) and occludin, and reducing the mucus thickness layer in U adult rats.
32388252	7	35	theme	tight-junction	1205:1218	arg1	occludin					1282:1289	occludin	1282:1289	occludin	1282:1289	Food-restriction increased intestinal permeability causing disorganization on the tight-junction proteins of colonic epithelium, Zonula Occludens-1 (ZO-1) and occludin, and reducing the mucus thickness layer in U adult rats.
32388252	7	35	theme	tight-junction	1205:1218	arg1	Occludens-1					1259:1269	Zonula Occludens-1	1252:1269	Zonula Occludens-1 (ZO-1)	1252:1276	Food-restriction increased intestinal permeability causing disorganization on the tight-junction proteins of colonic epithelium, Zonula Occludens-1 (ZO-1) and occludin, and reducing the mucus thickness layer in U adult rats.
32388252	11	36	theme	U	1716:1716	arg1	rats					1718:1721	U rats	1716:1721	U rats	1716:1721	Thus, gut dysbiosis and chronic endotoxemia observed in U rats, even before catch-up growth, might anticipate a pro-inflammatory milieu promoting metabolic diseases when fed hyperlipidic diets.
32388252	4	37	theme	white	731:735	arg1	tissue					745:750	white adipose tissue	731:750	white adipose tissue	731:750	HF-feeding induced glucose intolerance (P<.05), insulin resistance (P<.001), and white adipose tissue inflammation (P<.001) in UHF rats compared to CHF.
32388252	10	38	theme	C	1631:1631	arg1	P<.001					1640:1645	P<.001	1640:1645	P<.001	1640:1645	Even more, serum lipopolysaccharides were already elevated in U rats compared to C group (P<.001) at weaning.
32388252	10	38	theme	C	1631:1631	arg1	group					1633:1637	C group	1631:1637	C group (P<.001)	1631:1646	Even more, serum lipopolysaccharides were already elevated in U rats compared to C group (P<.001) at weaning.
32388252	1	39	theme	increased	232:240	arg1	risk					242:245	increased risk	232:245	increased risk of metabolic syndrome in adulthood	232:280	Nutritional restriction early in life followed by catch-up growth has been associated with increased risk of metabolic syndrome in adulthood.
32388252	9	40	theme	adult	1538:1542	arg1	rats					1544:1547	both U and UHF adult rats	1523:1547	both U and UHF adult rats	1523:1547	This event was correlated with increased circulating levels of bacterial lipopolysaccharides in both U and UHF adult rats.
32388252	6	41	theme	relative	1007:1014	arg1	abundance					1016:1024	relative abundance	1007:1024	relative abundance of Akkermansia	1007:1039	Although relative abundance of Akkermansia remained elevated to adulthood in U rats, HFD normalized its levels to C and CHF.
32388252	5	42	from	expansion	907:915	arg1	pups					992:995	U pups	990:995	U pups	990:995	Analyses of gut microbial composition before catch-up growth revealed reduced F/B ratio and significant expansion of the mucolytic genera Akkermansia (P<.05) and Desulfovibrio (P<.05) in U pups.
32388252	7	43	theme	intestinal	1150:1159	arg1	permeability					1161:1172	intestinal permeability	1150:1172	intestinal permeability	1150:1172	Food-restriction increased intestinal permeability causing disorganization on the tight-junction proteins of colonic epithelium, Zonula Occludens-1 (ZO-1) and occludin, and reducing the mucus thickness layer in U adult rats.
32388252	11	44	located	observed	1704:1711	arg1	rats					1718:1721	U rats	1716:1721	U rats	1716:1721	Thus, gut dysbiosis and chronic endotoxemia observed in U rats, even before catch-up growth, might anticipate a pro-inflammatory milieu promoting metabolic diseases when fed hyperlipidic diets.
32388252	11	44	located	observed	1704:1711	arg2	dysbiosis					1670:1678	gut dysbiosis	1666:1678	gut dysbiosis	1666:1678	Thus, gut dysbiosis and chronic endotoxemia observed in U rats, even before catch-up growth, might anticipate a pro-inflammatory milieu promoting metabolic diseases when fed hyperlipidic diets.
32388252	1	45	theme	syndrome	260:267	arg1	risk					242:245	increased risk	232:245	increased risk of metabolic syndrome in adulthood	232:280	Nutritional restriction early in life followed by catch-up growth has been associated with increased risk of metabolic syndrome in adulthood.
32388252	2	46	theme	responsible	354:364	arg1	mechanisms					343:352	the mechanisms	339:352	the mechanisms responsible of this predisposition gut microbiome	339:402	To elucidate whether altered gut colonization underlies the mechanisms responsible of this predisposition gut microbiome was studied before or afterwards catch-up growth.
32388252	7	47	from	disorganization	1182:1196	arg1	proteins					1220:1227	the tight-junction proteins	1201:1227	the tight-junction proteins of colonic epithelium	1201:1249	Food-restriction increased intestinal permeability causing disorganization on the tight-junction proteins of colonic epithelium, Zonula Occludens-1 (ZO-1) and occludin, and reducing the mucus thickness layer in U adult rats.
32388252	7	47	from	disorganization	1182:1196	arg1	occludin					1282:1289	occludin	1282:1289	occludin	1282:1289	Food-restriction increased intestinal permeability causing disorganization on the tight-junction proteins of colonic epithelium, Zonula Occludens-1 (ZO-1) and occludin, and reducing the mucus thickness layer in U adult rats.
32388252	7	47	from	disorganization	1182:1196	arg1	Occludens-1					1259:1269	Zonula Occludens-1	1252:1269	Zonula Occludens-1 (ZO-1)	1252:1276	Food-restriction increased intestinal permeability causing disorganization on the tight-junction proteins of colonic epithelium, Zonula Occludens-1 (ZO-1) and occludin, and reducing the mucus thickness layer in U adult rats.
32388252	7	48	theme	adult	1336:1340	arg1	rats					1342:1345	U adult rats	1334:1345	U adult rats	1334:1345	Food-restriction increased intestinal permeability causing disorganization on the tight-junction proteins of colonic epithelium, Zonula Occludens-1 (ZO-1) and occludin, and reducing the mucus thickness layer in U adult rats.
32388252	0	49	theme	nutrient-induced	107:122	arg1	growth					133:138	nutrient-induced catch-up growth	107:138	nutrient-induced catch-up growth	107:138	Gut DYSBIOSIS and altered barrier function precedes the appearance of metabolic syndrome in a rat model of nutrient-induced catch-up growth.
32388252	2	50	theme	altered	304:310	arg1	colonization					316:327	altered gut colonization	304:327	altered gut colonization	304:327	To elucidate whether altered gut colonization underlies the mechanisms responsible of this predisposition gut microbiome was studied before or afterwards catch-up growth.
32388252	1	51	theme	catch-up	191:198	arg1	growth					200:205	catch-up growth	191:205	catch-up growth	191:205	Nutritional restriction early in life followed by catch-up growth has been associated with increased risk of metabolic syndrome in adulthood.
32388252	3	52	theme	high-fat	561:568	arg1	HFD					576:578	HFD	576:578	HFD	576:578	Offspring of dams fed ad libitum (C) or undernourished during pregnancy and suckling (U), were weaned onto high-fat diet (HFD) for 22 weeks (CHF and UHF, respectively) or continued on their diet.
32388252	3	52	theme	high-fat	561:568	arg1	diet					570:573	high-fat diet	561:573	high-fat diet (HFD) for 22 weeks (CHF and UHF, respectively)	561:620	Offspring of dams fed ad libitum (C) or undernourished during pregnancy and suckling (U), were weaned onto high-fat diet (HFD) for 22 weeks (CHF and UHF, respectively) or continued on their diet.
32388252	2	53	theme	gut	389:391	arg1	microbiome					393:402	this predisposition gut microbiome	369:402	this predisposition gut microbiome	369:402	To elucidate whether altered gut colonization underlies the mechanisms responsible of this predisposition gut microbiome was studied before or afterwards catch-up growth.
32388252	7	54	theme	thickness	1315:1323	arg1	layer					1325:1329	the mucus thickness layer	1305:1329	the mucus thickness layer in U adult rats	1305:1345	Food-restriction increased intestinal permeability causing disorganization on the tight-junction proteins of colonic epithelium, Zonula Occludens-1 (ZO-1) and occludin, and reducing the mucus thickness layer in U adult rats.
32388252	11	55	theme	gut	1666:1668	arg1	dysbiosis					1670:1678	gut dysbiosis	1666:1678	gut dysbiosis	1666:1678	Thus, gut dysbiosis and chronic endotoxemia observed in U rats, even before catch-up growth, might anticipate a pro-inflammatory milieu promoting metabolic diseases when fed hyperlipidic diets.
32388252	0	56	theme	growth	133:138	arg1	model					98:102	a rat model	92:102	a rat model of nutrient-induced catch-up growth	92:138	Gut DYSBIOSIS and altered barrier function precedes the appearance of metabolic syndrome in a rat model of nutrient-induced catch-up growth.
32388252	11	57	theme	hyperlipidic	1834:1845	arg1	diets					1847:1851	hyperlipidic diets	1834:1851	hyperlipidic diets	1834:1851	Thus, gut dysbiosis and chronic endotoxemia observed in U rats, even before catch-up growth, might anticipate a pro-inflammatory milieu promoting metabolic diseases when fed hyperlipidic diets.
32388252	3	58	dep	weeks	588:592	arg1	UHF					603:605	UHF	603:605	UHF	603:605	Offspring of dams fed ad libitum (C) or undernourished during pregnancy and suckling (U), were weaned onto high-fat diet (HFD) for 22 weeks (CHF and UHF, respectively) or continued on their diet.
32388252	3	58	dep	weeks	588:592	arg1	CHF					595:597	CHF	595:597	CHF	595:597	Offspring of dams fed ad libitum (C) or undernourished during pregnancy and suckling (U), were weaned onto high-fat diet (HFD) for 22 weeks (CHF and UHF, respectively) or continued on their diet.
32388252	3	58	dep	weeks	588:592	arg1	weeks					588:592	22 weeks	585:592	22 weeks (CHF and UHF, respectively)	585:620	Offspring of dams fed ad libitum (C) or undernourished during pregnancy and suckling (U), were weaned onto high-fat diet (HFD) for 22 weeks (CHF and UHF, respectively) or continued on their diet.
32388252	8	59	theme	ZO-1	1362:1365	arg1	levels					1352:1357	The levels	1348:1357	The levels of ZO-1 and occludin	1348:1378	The levels of ZO-1 and occludin were not recovered in U rats after HF-feeding.
32388252	5	60	theme	microbial	819:827	arg1	composition					829:839	gut microbial composition	815:839	gut microbial composition before catch-up growth	815:862	Analyses of gut microbial composition before catch-up growth revealed reduced F/B ratio and significant expansion of the mucolytic genera Akkermansia (P<.05) and Desulfovibrio (P<.05) in U pups.
32388252	9	61	from	rats	1544:1547	arg1	levels					1480:1485	increased circulating levels	1458:1485	increased circulating levels of bacterial lipopolysaccharides in both U and UHF adult rats	1458:1547	This event was correlated with increased circulating levels of bacterial lipopolysaccharides in both U and UHF adult rats.
32388252	8	62	theme	occludin	1371:1378	arg1	levels					1352:1357	The levels	1348:1357	The levels of ZO-1 and occludin	1348:1378	The levels of ZO-1 and occludin were not recovered in U rats after HF-feeding.
32388252	5	63	theme	Desulfovibrio	965:977	arg1	ratio					885:889	reduced F/B ratio	873:889	reduced F/B ratio	873:889	Analyses of gut microbial composition before catch-up growth revealed reduced F/B ratio and significant expansion of the mucolytic genera Akkermansia (P<.05) and Desulfovibrio (P<.05) in U pups.
32388252	5	63	theme	Desulfovibrio	965:977	arg1	expansion					907:915	significant expansion	895:915	significant expansion	895:915	Analyses of gut microbial composition before catch-up growth revealed reduced F/B ratio and significant expansion of the mucolytic genera Akkermansia (P<.05) and Desulfovibrio (P<.05) in U pups.
32388252	4	64	theme	glucose	669:675	arg1	P<.05					690:694	P<.05	690:694	P<.05	690:694	HF-feeding induced glucose intolerance (P<.05), insulin resistance (P<.001), and white adipose tissue inflammation (P<.001) in UHF rats compared to CHF.
32388252	4	64	theme	glucose	669:675	arg1	intolerance					677:687	glucose intolerance	669:687	glucose intolerance (P<.05)	669:695	HF-feeding induced glucose intolerance (P<.05), insulin resistance (P<.001), and white adipose tissue inflammation (P<.001) in UHF rats compared to CHF.
32388252	7	65	dep	increased	1140:1148	arg1	causing					1174:1180	causing	1174:1180	causing disorganization on the tight-junction proteins of colonic epithelium, Zonula Occludens-1 (ZO-1) and occludin,	1174:1290	Food-restriction increased intestinal permeability causing disorganization on the tight-junction proteins of colonic epithelium, Zonula Occludens-1 (ZO-1) and occludin, and reducing the mucus thickness layer in U adult rats.
32388252	7	65	dep	increased	1140:1148	arg1	reducing					1296:1303	reducing	1296:1303	reducing the mucus thickness layer in U adult rats	1296:1345	Food-restriction increased intestinal permeability causing disorganization on the tight-junction proteins of colonic epithelium, Zonula Occludens-1 (ZO-1) and occludin, and reducing the mucus thickness layer in U adult rats.
32388252	0	66	theme	syndrome	80:87	arg1	appearance					56:65	the appearance	52:65	the appearance of metabolic syndrome in a rat model of nutrient-induced catch-up growth	52:138	Gut DYSBIOSIS and altered barrier function precedes the appearance of metabolic syndrome in a rat model of nutrient-induced catch-up growth.
32388252	4	67	theme	insulin	698:704	arg1	resistance					706:715	insulin resistance	698:715	insulin resistance (P<.001)	698:724	HF-feeding induced glucose intolerance (P<.05), insulin resistance (P<.001), and white adipose tissue inflammation (P<.001) in UHF rats compared to CHF.
32388252	4	67	theme	insulin	698:704	arg1	P<.001					718:723	P<.001	718:723	P<.001	718:723	HF-feeding induced glucose intolerance (P<.05), insulin resistance (P<.001), and white adipose tissue inflammation (P<.001) in UHF rats compared to CHF.
32388252	5	68	theme	significant	895:905	arg1	expansion					907:915	significant expansion	895:915	significant expansion	895:915	Analyses of gut microbial composition before catch-up growth revealed reduced F/B ratio and significant expansion of the mucolytic genera Akkermansia (P<.05) and Desulfovibrio (P<.05) in U pups.
32388252	3	69	theme	dams	467:470	arg1	Offspring					454:462	Offspring	454:462	Offspring	454:462	Offspring of dams fed ad libitum (C) or undernourished during pregnancy and suckling (U), were weaned onto high-fat diet (HFD) for 22 weeks (CHF and UHF, respectively) or continued on their diet.
32388252	4	70	dep	intolerance	677:687	arg1	P<.001					766:771	P<.001	766:771	P<.001	766:771	HF-feeding induced glucose intolerance (P<.05), insulin resistance (P<.001), and white adipose tissue inflammation (P<.001) in UHF rats compared to CHF.
32388252	4	70	dep	intolerance	677:687	arg1	inflammation					752:763	inflammation	752:763	inflammation (P<.001)	752:772	HF-feeding induced glucose intolerance (P<.05), insulin resistance (P<.001), and white adipose tissue inflammation (P<.001) in UHF rats compared to CHF.
32388252	2	71	theme	microbiome	393:402	arg1	responsible					354:364	responsible	354:364	responsible	354:364	To elucidate whether altered gut colonization underlies the mechanisms responsible of this predisposition gut microbiome was studied before or afterwards catch-up growth.
32388252	0	72	theme	altered	18:24	arg1	function					34:41	altered barrier function	18:41	altered barrier function	18:41	Gut DYSBIOSIS and altered barrier function precedes the appearance of metabolic syndrome in a rat model of nutrient-induced catch-up growth.
32388252	9	73	theme	UHF	1534:1536	arg1	rats					1544:1547	both U and UHF adult rats	1523:1547	both U and UHF adult rats	1523:1547	This event was correlated with increased circulating levels of bacterial lipopolysaccharides in both U and UHF adult rats.
32388252	5	74	theme	reduced	873:879	arg1	ratio					885:889	reduced F/B ratio	873:889	reduced F/B ratio	873:889	Analyses of gut microbial composition before catch-up growth revealed reduced F/B ratio and significant expansion of the mucolytic genera Akkermansia (P<.05) and Desulfovibrio (P<.05) in U pups.
32388252	7	75	theme	colonic	1232:1238	arg1	epithelium					1240:1249	colonic epithelium	1232:1249	colonic epithelium	1232:1249	Food-restriction increased intestinal permeability causing disorganization on the tight-junction proteins of colonic epithelium, Zonula Occludens-1 (ZO-1) and occludin, and reducing the mucus thickness layer in U adult rats.
32388252	2	76	theme	catch-up	437:444	arg1	growth					446:451	catch-up growth	437:451	catch-up growth	437:451	To elucidate whether altered gut colonization underlies the mechanisms responsible of this predisposition gut microbiome was studied before or afterwards catch-up growth.
32388252	9	77	theme	bacterial	1490:1498	arg1	lipopolysaccharides					1500:1518	bacterial lipopolysaccharides	1490:1518	bacterial lipopolysaccharides in both U and UHF adult rats	1490:1547	This event was correlated with increased circulating levels of bacterial lipopolysaccharides in both U and UHF adult rats.
32388252	9	78	from	lipopolysaccharides	1500:1518	arg1	rats					1544:1547	both U and UHF adult rats	1523:1547	both U and UHF adult rats	1523:1547	This event was correlated with increased circulating levels of bacterial lipopolysaccharides in both U and UHF adult rats.
32388252	4	79	theme	adipose	737:743	arg1	tissue					745:750	white adipose tissue	731:750	white adipose tissue	731:750	HF-feeding induced glucose intolerance (P<.05), insulin resistance (P<.001), and white adipose tissue inflammation (P<.001) in UHF rats compared to CHF.
32388252	3	80	theme	ad	476:477	arg1	C					488:488	C	488:488	C	488:488	Offspring of dams fed ad libitum (C) or undernourished during pregnancy and suckling (U), were weaned onto high-fat diet (HFD) for 22 weeks (CHF and UHF, respectively) or continued on their diet.
32388252	3	80	theme	ad	476:477	arg1	libitum					479:485	ad libitum	476:485	ad libitum (C)	476:489	Offspring of dams fed ad libitum (C) or undernourished during pregnancy and suckling (U), were weaned onto high-fat diet (HFD) for 22 weeks (CHF and UHF, respectively) or continued on their diet.
32388252	5	81	theme	genera	934:939	arg1	P<.05					954:958	P<.05	954:958	P<.05	954:958	Analyses of gut microbial composition before catch-up growth revealed reduced F/B ratio and significant expansion of the mucolytic genera Akkermansia (P<.05) and Desulfovibrio (P<.05) in U pups.
32388252	5	81	theme	genera	934:939	arg1	Akkermansia					941:951	the mucolytic genera Akkermansia	920:951	the mucolytic genera Akkermansia (P<.05)	920:959	Analyses of gut microbial composition before catch-up growth revealed reduced F/B ratio and significant expansion of the mucolytic genera Akkermansia (P<.05) and Desulfovibrio (P<.05) in U pups.
32388252	9	82	theme	U	1528:1528	arg1	rats					1544:1547	both U and UHF adult rats	1523:1547	both U and UHF adult rats	1523:1547	This event was correlated with increased circulating levels of bacterial lipopolysaccharides in both U and UHF adult rats.
32388252	6	83	theme	Akkermansia	1029:1039	arg1	abundance					1016:1024	relative abundance	1007:1024	relative abundance of Akkermansia	1007:1039	Although relative abundance of Akkermansia remained elevated to adulthood in U rats, HFD normalized its levels to C and CHF.
32388252	5	84	theme	mucolytic	924:932	arg1	P<.05					954:958	P<.05	954:958	P<.05	954:958	Analyses of gut microbial composition before catch-up growth revealed reduced F/B ratio and significant expansion of the mucolytic genera Akkermansia (P<.05) and Desulfovibrio (P<.05) in U pups.
32388252	5	84	theme	mucolytic	924:932	arg1	Akkermansia					941:951	the mucolytic genera Akkermansia	920:951	the mucolytic genera Akkermansia (P<.05)	920:959	Analyses of gut microbial composition before catch-up growth revealed reduced F/B ratio and significant expansion of the mucolytic genera Akkermansia (P<.05) and Desulfovibrio (P<.05) in U pups.
32388252	11	85	theme	pro-inflammatory	1772:1787	arg1	milieu					1789:1794	a pro-inflammatory milieu	1770:1794	a pro-inflammatory milieu promoting metabolic diseases	1770:1823	Thus, gut dysbiosis and chronic endotoxemia observed in U rats, even before catch-up growth, might anticipate a pro-inflammatory milieu promoting metabolic diseases when fed hyperlipidic diets.
32388252	2	86	theme	predisposition	374:387	arg1	microbiome					393:402	this predisposition gut microbiome	369:402	this predisposition gut microbiome	369:402	To elucidate whether altered gut colonization underlies the mechanisms responsible of this predisposition gut microbiome was studied before or afterwards catch-up growth.
32306134	2	0	theme	Protaetia	100:108	arg1	seulensis					122:130	Protaetia brevitarsis seulensis	100:130	Protaetia brevitarsis seulensis	100:130	nov., isolated from gut of larvae of Protaetia brevitarsis seulensis.
32306134	6	1	theme	sequence	682:689	arg1	analysis					691:698	the 16S rRNA gene sequence analysis	664:698	the 16S rRNA gene sequence analysis	664:698	Based on the 16S rRNA gene sequence analysis, strain FW10M-9T was classified into the genus Xylanimonas revealing 96.9-98.5% sequence similarities with the Xylanimonas species.
32306134	7	2	dep	species	883:889	arg1	107786T					918:924	107786T	918:924	107786T	918:924	The ANI values of strain FW10M-9T with the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T and Xylanimonas cellulosilytica DSM 15894T was 81.5%, 81.2%, 81.0% and 84.1%, respectively, and the dDDH values estimated by GGDC was 24.3%, 24.3%, 29.3% and 28.1%, respectively.
32306134	7	2	dep	species	883:889	arg1	allomyrinae					939:949	allomyrinae	939:949	allomyrinae	939:949	The ANI values of strain FW10M-9T with the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T and Xylanimonas cellulosilytica DSM 15894T was 81.5%, 81.2%, 81.0% and 84.1%, respectively, and the dDDH values estimated by GGDC was 24.3%, 24.3%, 29.3% and 28.1%, respectively.
32306134	7	2	dep	species	883:889	arg1	Isoptericola					961:972	Isoptericola	961:972	Isoptericola	961:972	The ANI values of strain FW10M-9T with the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T and Xylanimonas cellulosilytica DSM 15894T was 81.5%, 81.2%, 81.0% and 84.1%, respectively, and the dDDH values estimated by GGDC was 24.3%, 24.3%, 29.3% and 28.1%, respectively.
32306134	7	3	theme	variabilis	974:983	arg1	11754T					989:994	the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T	871:994	the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T	871:994	The ANI values of strain FW10M-9T with the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T and Xylanimonas cellulosilytica DSM 15894T was 81.5%, 81.2%, 81.0% and 84.1%, respectively, and the dDDH values estimated by GGDC was 24.3%, 24.3%, 29.3% and 28.1%, respectively.
32306134	8	4	theme	name	1329:1332	arg1	sp					1357:1358	the name Lactococcus protaetiae sp	1325:1358	the name Lactococcus protaetiae sp	1325:1358	On the basis of the phenotypic and genotypic data, it is proposed that strain 2DFWM-2T represents a novel species of the genus Lactococcus, for which the name Lactococcus protaetiae sp.
32306134	10	5	theme	protaetiae	1548:1557	arg1	sp					1559:1560	the name Xylanimonas protaetiae sp	1527:1560	the name Xylanimonas protaetiae sp	1527:1560	And, strain FW10M-9T represents a novel species of the genus Xylanimonas, for which the name Xylanimonas protaetiae sp.
32306134	2	6	theme	larvae	90:95	arg1	gut					83:85	gut	83:85	gut of larvae of Protaetia brevitarsis seulensis	83:130	nov., isolated from gut of larvae of Protaetia brevitarsis seulensis.
32306134	5	7	theme	Lactococcus	584:594	arg1	1JSPR-7T					608:615	Lactococcus allomyrinae 1JSPR-7T	584:615	Lactococcus allomyrinae 1JSPR-7T	584:615	ANI and dDDH values between 2DFWM-2T and Lactococcus allomyrinae 1JSPR-7T were 93.30% and 53.20%, respectively.
32306134	7	8	theme	strain	850:855	arg1	FW10M-9T					857:864	strain FW10M-9T	850:864	strain FW10M-9T with the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T and Xylanimonas cellulosilytica DSM 15894T	850:1037	The ANI values of strain FW10M-9T with the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T and Xylanimonas cellulosilytica DSM 15894T was 81.5%, 81.2%, 81.0% and 84.1%, respectively, and the dDDH values estimated by GGDC was 24.3%, 24.3%, 29.3% and 28.1%, respectively.
32306134	7	9	theme	JCM	985:987	arg1	11754T					989:994	the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T	871:994	the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T	871:994	The ANI values of strain FW10M-9T with the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T and Xylanimonas cellulosilytica DSM 15894T was 81.5%, 81.2%, 81.0% and 84.1%, respectively, and the dDDH values estimated by GGDC was 24.3%, 24.3%, 29.3% and 28.1%, respectively.
32306134	4	10	theme	strain	384:389	arg1	2DFWM-2T					391:398	strain 2DFWM-2T	384:398	strain 2DFWM-2T	384:398	16S rRNA and rpoB gene sequences showed that strain 2DFWM-2T formed a separate branch with Lactococcus allomyrinae 1JSPR-7T in the genus Lactococcus, adjacent to a group of Lactococcus lactis subspecies.
32306134	6	11	theme	gene	677:680	arg1	analysis					691:698	the 16S rRNA gene sequence analysis	664:698	the 16S rRNA gene sequence analysis	664:698	Based on the 16S rRNA gene sequence analysis, strain FW10M-9T was classified into the genus Xylanimonas revealing 96.9-98.5% sequence similarities with the Xylanimonas species.
32306134	3	12	attach	isolated	195:202	arg1	gut					209:211	gut	209:211	gut of larva of Protaetia brevitarsis seulensis grown at the National Institute of Agricultural Sciences, Wanju-gun, South Korea	209:336	Two bacterial strains, designated 2DFWM-2T and FW10M-9T, were isolated from gut of larva of Protaetia brevitarsis seulensis grown at the National Institute of Agricultural Sciences, Wanju-gun, South Korea.
32306134	3	12	attach	isolated	195:202	arg2	strains					147:153	Two bacterial strains	133:153	Two bacterial strains	133:153	Two bacterial strains, designated 2DFWM-2T and FW10M-9T, were isolated from gut of larva of Protaetia brevitarsis seulensis grown at the National Institute of Agricultural Sciences, Wanju-gun, South Korea.
32306134	7	13	theme	ANI	836:838	arg1	%					1047:1047	81.5%	1043:1047	81.5%	1043:1047	The ANI values of strain FW10M-9T with the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T and Xylanimonas cellulosilytica DSM 15894T was 81.5%, 81.2%, 81.0% and 84.1%, respectively, and the dDDH values estimated by GGDC was 24.3%, 24.3%, 29.3% and 28.1%, respectively.
32306134	7	13	theme	ANI	836:838	arg1	values					840:845	The ANI values	832:845	The ANI values of strain FW10M-9T with the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T and Xylanimonas cellulosilytica DSM 15894T	832:1037	The ANI values of strain FW10M-9T with the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T and Xylanimonas cellulosilytica DSM 15894T was 81.5%, 81.2%, 81.0% and 84.1%, respectively, and the dDDH values estimated by GGDC was 24.3%, 24.3%, 29.3% and 28.1%, respectively.
32306134	3	14	theme	Protaetia	225:233	arg1	seulensis					247:255	Protaetia brevitarsis seulensis	225:255	Protaetia brevitarsis seulensis grown at the National Institute of Agricultural Sciences, Wanju-gun, South Korea	225:336	Two bacterial strains, designated 2DFWM-2T and FW10M-9T, were isolated from gut of larva of Protaetia brevitarsis seulensis grown at the National Institute of Agricultural Sciences, Wanju-gun, South Korea.
32306134	5	15	theme	allomyrinae	596:606	arg1	1JSPR-7T					608:615	Lactococcus allomyrinae 1JSPR-7T	584:615	Lactococcus allomyrinae 1JSPR-7T	584:615	ANI and dDDH values between 2DFWM-2T and Lactococcus allomyrinae 1JSPR-7T were 93.30% and 53.20%, respectively.
32306134	9	16	theme	= KACC	1412:1417	arg1	2DFWM-2T					1402:1409	2DFWM-2T	1402:1409	2DFWM-2T (= KACC 19320T = NBRC 113069T)	1402:1440	nov. is proposed, and the type strain is 2DFWM-2T (= KACC 19320T = NBRC 113069T).
32306134	9	16	theme	= KACC	1412:1417	arg1	113069T					1433:1439	= KACC 19320T = NBRC 113069T	1412:1439	= KACC 19320T = NBRC 113069T	1412:1439	nov. is proposed, and the type strain is 2DFWM-2T (= KACC 19320T = NBRC 113069T).
32306134	6	17	theme	rRNA	672:675	arg1	analysis					691:698	the 16S rRNA gene sequence analysis	664:698	the 16S rRNA gene sequence analysis	664:698	Based on the 16S rRNA gene sequence analysis, strain FW10M-9T was classified into the genus Xylanimonas revealing 96.9-98.5% sequence similarities with the Xylanimonas species.
32306134	3	18	theme	Korea	332:336	arg1	Institute					279:287	the National Institute	266:287	the National Institute of Agricultural Sciences, Wanju-gun, South Korea	266:336	Two bacterial strains, designated 2DFWM-2T and FW10M-9T, were isolated from gut of larva of Protaetia brevitarsis seulensis grown at the National Institute of Agricultural Sciences, Wanju-gun, South Korea.
32306134	4	19	theme	genus	470:474	arg1	Lactococcus					476:486	the genus Lactococcus	466:486	the genus Lactococcus	466:486	16S rRNA and rpoB gene sequences showed that strain 2DFWM-2T formed a separate branch with Lactococcus allomyrinae 1JSPR-7T in the genus Lactococcus, adjacent to a group of Lactococcus lactis subspecies.
32306134	3	20	theme	brevitarsis	235:245	arg1	seulensis					247:255	Protaetia brevitarsis seulensis	225:255	Protaetia brevitarsis seulensis grown at the National Institute of Agricultural Sciences, Wanju-gun, South Korea	225:336	Two bacterial strains, designated 2DFWM-2T and FW10M-9T, were isolated from gut of larva of Protaetia brevitarsis seulensis grown at the National Institute of Agricultural Sciences, Wanju-gun, South Korea.
32306134	9	21	theme	19320T = NBRC	1419:1431	arg1	2DFWM-2T					1402:1409	2DFWM-2T	1402:1409	2DFWM-2T (= KACC 19320T = NBRC 113069T)	1402:1440	nov. is proposed, and the type strain is 2DFWM-2T (= KACC 19320T = NBRC 113069T).
32306134	9	21	theme	19320T = NBRC	1419:1431	arg1	113069T					1433:1439	= KACC 19320T = NBRC 113069T	1412:1439	= KACC 19320T = NBRC 113069T	1412:1439	nov. is proposed, and the type strain is 2DFWM-2T (= KACC 19320T = NBRC 113069T).
32306134	6	22	theme	16S	668:670	arg1	analysis					691:698	the 16S rRNA gene sequence analysis	664:698	the 16S rRNA gene sequence analysis	664:698	Based on the 16S rRNA gene sequence analysis, strain FW10M-9T was classified into the genus Xylanimonas revealing 96.9-98.5% sequence similarities with the Xylanimonas species.
32306134	10	23	theme	Xylanimonas	1504:1514	arg1	species					1483:1489	a novel species	1475:1489	a novel species	1475:1489	And, strain FW10M-9T represents a novel species of the genus Xylanimonas, for which the name Xylanimonas protaetiae sp.
32306134	0	24	theme	protaetiae	12:21	arg1	sp					23:24	Lactococcus protaetiae sp	0:24	Lactococcus protaetiae sp.	0:25	Lactococcus protaetiae sp.
32306134	2	25	theme	seulensis	122:130	arg1	larvae					90:95	larvae	90:95	larvae of Protaetia brevitarsis seulensis	90:130	nov., isolated from gut of larvae of Protaetia brevitarsis seulensis.
32306134	3	26	theme	larva	216:220	arg1	gut					209:211	gut	209:211	gut of larva of Protaetia brevitarsis seulensis grown at the National Institute of Agricultural Sciences, Wanju-gun, South Korea	209:336	Two bacterial strains, designated 2DFWM-2T and FW10M-9T, were isolated from gut of larva of Protaetia brevitarsis seulensis grown at the National Institute of Agricultural Sciences, Wanju-gun, South Korea.
32306134	5	27	theme	dDDH	551:554	arg1	values					556:561	ANI and dDDH values	543:561	ANI and dDDH values between 2DFWM-2T and Lactococcus allomyrinae 1JSPR-7T	543:615	ANI and dDDH values between 2DFWM-2T and Lactococcus allomyrinae 1JSPR-7T were 93.30% and 53.20%, respectively.
32306134	10	28	theme	strain	1448:1453	arg1	FW10M-9T					1455:1462	strain FW10M-9T	1448:1462	strain FW10M-9T	1448:1462	And, strain FW10M-9T represents a novel species of the genus Xylanimonas, for which the name Xylanimonas protaetiae sp.
32306134	10	29	theme	genus	1498:1502	arg1	Xylanimonas					1504:1514	the genus Xylanimonas	1494:1514	the genus Xylanimonas	1494:1514	And, strain FW10M-9T represents a novel species of the genus Xylanimonas, for which the name Xylanimonas protaetiae sp.
32306134	0	30	theme	Lactococcus	0:10	arg1	sp					23:24	Lactococcus protaetiae sp	0:24	Lactococcus protaetiae sp.	0:25	Lactococcus protaetiae sp.
32306134	4	31	theme	gene	357:360	arg1	sequences					362:370	16S rRNA and rpoB gene sequences	339:370	sequences	362:370	16S rRNA and rpoB gene sequences showed that strain 2DFWM-2T formed a separate branch with Lactococcus allomyrinae 1JSPR-7T in the genus Lactococcus, adjacent to a group of Lactococcus lactis subspecies.
32306134	8	32	theme	strain	1246:1251	arg1	2DFWM-2T					1253:1260	strain 2DFWM-2T	1246:1260	strain 2DFWM-2T	1246:1260	On the basis of the phenotypic and genotypic data, it is proposed that strain 2DFWM-2T represents a novel species of the genus Lactococcus, for which the name Lactococcus protaetiae sp.
32306134	4	33	with	branch	418:423	arg1	1JSPR-7T					454:461	Lactococcus allomyrinae 1JSPR-7T	430:461	Lactococcus allomyrinae 1JSPR-7T	430:461	16S rRNA and rpoB gene sequences showed that strain 2DFWM-2T formed a separate branch with Lactococcus allomyrinae 1JSPR-7T in the genus Lactococcus, adjacent to a group of Lactococcus lactis subspecies.
32306134	6	34	with	similarities	789:800	arg1	species					823:829	the Xylanimonas species	807:829	the Xylanimonas species	807:829	Based on the 16S rRNA gene sequence analysis, strain FW10M-9T was classified into the genus Xylanimonas revealing 96.9-98.5% sequence similarities with the Xylanimonas species.
32306134	4	35	theme	lactis	524:529	arg1	group					503:507	a group	501:507	a group of Lactococcus lactis	501:529	16S rRNA and rpoB gene sequences showed that strain 2DFWM-2T formed a separate branch with Lactococcus allomyrinae 1JSPR-7T in the genus Lactococcus, adjacent to a group of Lactococcus lactis subspecies.
32306134	4	35	theme	lactis	524:529	arg1	lactis					524:529	Lactococcus lactis	512:529	Lactococcus lactis	512:529	16S rRNA and rpoB gene sequences showed that strain 2DFWM-2T formed a separate branch with Lactococcus allomyrinae 1JSPR-7T in the genus Lactococcus, adjacent to a group of Lactococcus lactis subspecies.
32306134	4	36	theme	rpoB	352:355	arg1	sequences					362:370	16S rRNA and rpoB gene sequences	339:370	sequences	362:370	16S rRNA and rpoB gene sequences showed that strain 2DFWM-2T formed a separate branch with Lactococcus allomyrinae 1JSPR-7T in the genus Lactococcus, adjacent to a group of Lactococcus lactis subspecies.
32306134	6	37	theme	sequence	780:787	arg1	similarities					789:800	96.9-98.5% sequence similarities	769:800	96.9-98.5% sequence similarities with the Xylanimonas species	769:829	Based on the 16S rRNA gene sequence analysis, strain FW10M-9T was classified into the genus Xylanimonas revealing 96.9-98.5% sequence similarities with the Xylanimonas species.
32306134	10	38	theme	name	1531:1534	arg1	sp					1559:1560	the name Xylanimonas protaetiae sp	1527:1560	the name Xylanimonas protaetiae sp	1527:1560	And, strain FW10M-9T represents a novel species of the genus Xylanimonas, for which the name Xylanimonas protaetiae sp.
32306134	6	39	theme	genus	741:745	arg1	Xylanimonas					747:757	the genus Xylanimonas	737:757	the genus Xylanimonas	737:757	Based on the 16S rRNA gene sequence analysis, strain FW10M-9T was classified into the genus Xylanimonas revealing 96.9-98.5% sequence similarities with the Xylanimonas species.
32306134	11	40	theme	19331T = NBRC	1621:1633	arg1	FW10M-9T					1604:1611	FW10M-9T	1604:1611	FW10M-9T (= KACC 19331T = NBRC 113053T)	1604:1642	nov. is proposed, and the type strain is FW10M-9T (= KACC 19331T = NBRC 113053T).
32306134	11	40	theme	19331T = NBRC	1621:1633	arg1	113053T					1635:1641	= KACC 19331T = NBRC 113053T	1614:1641	= KACC 19331T = NBRC 113053T	1614:1641	nov. is proposed, and the type strain is FW10M-9T (= KACC 19331T = NBRC 113053T).
32306134	7	41	theme	dDDH	1096:1099	arg1	values					1101:1106	the dDDH values	1092:1106	the dDDH values estimated by GGDC	1092:1124	The ANI values of strain FW10M-9T with the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T and Xylanimonas cellulosilytica DSM 15894T was 81.5%, 81.2%, 81.0% and 84.1%, respectively, and the dDDH values estimated by GGDC was 24.3%, 24.3%, 29.3% and 28.1%, respectively.
32306134	7	41	theme	dDDH	1096:1099	arg1	%					1134:1134	24.3%	1130:1134	24.3%	1130:1134	The ANI values of strain FW10M-9T with the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T and Xylanimonas cellulosilytica DSM 15894T was 81.5%, 81.2%, 81.0% and 84.1%, respectively, and the dDDH values estimated by GGDC was 24.3%, 24.3%, 29.3% and 28.1%, respectively.
32306134	7	42	dep	Xylanimonas	1000:1010	arg1	cellulosilytica					1012:1026	cellulosilytica	1012:1026	cellulosilytica	1012:1026	The ANI values of strain FW10M-9T with the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T and Xylanimonas cellulosilytica DSM 15894T was 81.5%, 81.2%, 81.0% and 84.1%, respectively, and the dDDH values estimated by GGDC was 24.3%, 24.3%, 29.3% and 28.1%, respectively.
32306134	3	43	theme	seulensis	247:255	arg1	larva					216:220	larva	216:220	larva of Protaetia brevitarsis seulensis grown at the National Institute of Agricultural Sciences, Wanju-gun, South Korea	216:336	Two bacterial strains, designated 2DFWM-2T and FW10M-9T, were isolated from gut of larva of Protaetia brevitarsis seulensis grown at the National Institute of Agricultural Sciences, Wanju-gun, South Korea.
32306134	11	44	theme	= KACC	1614:1619	arg1	FW10M-9T					1604:1611	FW10M-9T	1604:1611	FW10M-9T (= KACC 19331T = NBRC 113053T)	1604:1642	nov. is proposed, and the type strain is FW10M-9T (= KACC 19331T = NBRC 113053T).
32306134	11	44	theme	= KACC	1614:1619	arg1	113053T					1635:1641	= KACC 19331T = NBRC 113053T	1614:1641	= KACC 19331T = NBRC 113053T	1614:1641	nov. is proposed, and the type strain is FW10M-9T (= KACC 19331T = NBRC 113053T).
32306134	7	45	theme	81.2	1050:1053	arg1	%					1054:1054	81.2%	1050:1054	81.2%	1050:1054	The ANI values of strain FW10M-9T with the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T and Xylanimonas cellulosilytica DSM 15894T was 81.5%, 81.2%, 81.0% and 84.1%, respectively, and the dDDH values estimated by GGDC was 24.3%, 24.3%, 29.3% and 28.1%, respectively.
32306134	4	46	theme	allomyrinae	442:452	arg1	1JSPR-7T					454:461	Lactococcus allomyrinae 1JSPR-7T	430:461	Lactococcus allomyrinae 1JSPR-7T	430:461	16S rRNA and rpoB gene sequences showed that strain 2DFWM-2T formed a separate branch with Lactococcus allomyrinae 1JSPR-7T in the genus Lactococcus, adjacent to a group of Lactococcus lactis subspecies.
32306134	8	47	theme	novel	1275:1279	arg1	species					1281:1287	a novel species	1273:1287	a novel species	1273:1287	On the basis of the phenotypic and genotypic data, it is proposed that strain 2DFWM-2T represents a novel species of the genus Lactococcus, for which the name Lactococcus protaetiae sp.
32306134	10	48	theme	novel	1477:1481	arg1	species					1483:1489	a novel species	1475:1489	a novel species	1475:1489	And, strain FW10M-9T represents a novel species of the genus Xylanimonas, for which the name Xylanimonas protaetiae sp.
32306134	6	49	theme	%	778:778	arg1	similarities					789:800	96.9-98.5% sequence similarities	769:800	96.9-98.5% sequence similarities with the Xylanimonas species	769:829	Based on the 16S rRNA gene sequence analysis, strain FW10M-9T was classified into the genus Xylanimonas revealing 96.9-98.5% sequence similarities with the Xylanimonas species.
32306134	4	50	theme	Lactococcus	430:440	arg1	1JSPR-7T					454:461	Lactococcus allomyrinae 1JSPR-7T	430:461	Lactococcus allomyrinae 1JSPR-7T	430:461	16S rRNA and rpoB gene sequences showed that strain 2DFWM-2T formed a separate branch with Lactococcus allomyrinae 1JSPR-7T in the genus Lactococcus, adjacent to a group of Lactococcus lactis subspecies.
32306134	7	51	theme	DSM	1028:1030	arg1	15894T					1032:1037	Xylanimonas cellulosilytica DSM 15894T	1000:1037	Xylanimonas cellulosilytica DSM 15894T	1000:1037	The ANI values of strain FW10M-9T with the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T and Xylanimonas cellulosilytica DSM 15894T was 81.5%, 81.2%, 81.0% and 84.1%, respectively, and the dDDH values estimated by GGDC was 24.3%, 24.3%, 29.3% and 28.1%, respectively.
32306134	8	52	dep	data	1220:1223	arg1	basis					1182:1186	basis	1182:1186	basis	1182:1186	On the basis of the phenotypic and genotypic data, it is proposed that strain 2DFWM-2T represents a novel species of the genus Lactococcus, for which the name Lactococcus protaetiae sp.
32306134	8	52	dep	data	1220:1223	arg1	the					1178:1180	the	1178:1180	the	1178:1180	On the basis of the phenotypic and genotypic data, it is proposed that strain 2DFWM-2T represents a novel species of the genus Lactococcus, for which the name Lactococcus protaetiae sp.
32306134	4	53	theme	adjacent	489:496	arg1	subspecies					531:540	adjacent to a group of Lactococcus lactis subspecies	489:540	adjacent to a group of Lactococcus lactis subspecies	489:540	16S rRNA and rpoB gene sequences showed that strain 2DFWM-2T formed a separate branch with Lactococcus allomyrinae 1JSPR-7T in the genus Lactococcus, adjacent to a group of Lactococcus lactis subspecies.
32306134	4	54	theme	Lactococcus	512:522	arg1	lactis					524:529	Lactococcus lactis	512:529	Lactococcus lactis	512:529	16S rRNA and rpoB gene sequences showed that strain 2DFWM-2T formed a separate branch with Lactococcus allomyrinae 1JSPR-7T in the genus Lactococcus, adjacent to a group of Lactococcus lactis subspecies.
32306134	7	55	with	FW10M-9T	857:864	arg1	15894T					1032:1037	Xylanimonas cellulosilytica DSM 15894T	1000:1037	Xylanimonas cellulosilytica DSM 15894T	1000:1037	The ANI values of strain FW10M-9T with the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T and Xylanimonas cellulosilytica DSM 15894T was 81.5%, 81.2%, 81.0% and 84.1%, respectively, and the dDDH values estimated by GGDC was 24.3%, 24.3%, 29.3% and 28.1%, respectively.
32306134	7	55	with	FW10M-9T	857:864	arg1	11754T					989:994	the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T	871:994	the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T	871:994	The ANI values of strain FW10M-9T with the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T and Xylanimonas cellulosilytica DSM 15894T was 81.5%, 81.2%, 81.0% and 84.1%, respectively, and the dDDH values estimated by GGDC was 24.3%, 24.3%, 29.3% and 28.1%, respectively.
32306134	8	56	theme	protaetiae	1346:1355	arg1	sp					1357:1358	the name Lactococcus protaetiae sp	1325:1358	the name Lactococcus protaetiae sp	1325:1358	On the basis of the phenotypic and genotypic data, it is proposed that strain 2DFWM-2T represents a novel species of the genus Lactococcus, for which the name Lactococcus protaetiae sp.
32306134	4	57	theme	16S	339:341	arg1	rRNA					343:346	16S rRNA and rpoB gene sequences	339:370	rRNA	343:346	16S rRNA and rpoB gene sequences showed that strain 2DFWM-2T formed a separate branch with Lactococcus allomyrinae 1JSPR-7T in the genus Lactococcus, adjacent to a group of Lactococcus lactis subspecies.
32306134	8	58	theme	Lactococcus	1334:1344	arg1	sp					1357:1358	the name Lactococcus protaetiae sp	1325:1358	the name Lactococcus protaetiae sp	1325:1358	On the basis of the phenotypic and genotypic data, it is proposed that strain 2DFWM-2T represents a novel species of the genus Lactococcus, for which the name Lactococcus protaetiae sp.
32306134	7	59	theme	species	883:889	arg1	11754T					989:994	the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T	871:994	the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T	871:994	The ANI values of strain FW10M-9T with the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T and Xylanimonas cellulosilytica DSM 15894T was 81.5%, 81.2%, 81.0% and 84.1%, respectively, and the dDDH values estimated by GGDC was 24.3%, 24.3%, 29.3% and 28.1%, respectively.
32306134	3	60	theme	National	270:277	arg1	Institute					279:287	the National Institute	266:287	the National Institute of Agricultural Sciences, Wanju-gun, South Korea	266:336	Two bacterial strains, designated 2DFWM-2T and FW10M-9T, were isolated from gut of larva of Protaetia brevitarsis seulensis grown at the National Institute of Agricultural Sciences, Wanju-gun, South Korea.
32306134	6	61	theme	Xylanimonas	811:821	arg1	species					823:829	the Xylanimonas species	807:829	the Xylanimonas species	807:829	Based on the 16S rRNA gene sequence analysis, strain FW10M-9T was classified into the genus Xylanimonas revealing 96.9-98.5% sequence similarities with the Xylanimonas species.
32306134	8	62	theme	Lactococcus	1302:1312	arg1	species					1281:1287	a novel species	1273:1287	a novel species	1273:1287	On the basis of the phenotypic and genotypic data, it is proposed that strain 2DFWM-2T represents a novel species of the genus Lactococcus, for which the name Lactococcus protaetiae sp.
32306134	5	63	theme	ANI	543:545	arg1	values					556:561	ANI and dDDH values	543:561	ANI and dDDH values between 2DFWM-2T and Lactococcus allomyrinae 1JSPR-7T	543:615	ANI and dDDH values between 2DFWM-2T and Lactococcus allomyrinae 1JSPR-7T were 93.30% and 53.20%, respectively.
32306134	1	64	dep	sp	59:60	arg1	Xylanimonas					36:46	Xylanimonas	36:46	Xylanimonas	36:46	nov. and Xylanimonas protaetiae sp.
32306134	1	64	dep	sp	59:60	arg1	nov.					27:30	nov.	27:30	nov.	27:30	nov. and Xylanimonas protaetiae sp.
32306134	11	65	theme	type	1589:1592	arg1	FW10M-9T					1604:1611	FW10M-9T	1604:1611	FW10M-9T (= KACC 19331T = NBRC 113053T)	1604:1642	nov. is proposed, and the type strain is FW10M-9T (= KACC 19331T = NBRC 113053T).
32306134	11	65	theme	type	1589:1592	arg1	strain					1594:1599	the type strain	1585:1599	the type strain	1585:1599	nov. is proposed, and the type strain is FW10M-9T (= KACC 19331T = NBRC 113053T).
32306134	7	66	theme	Xylanimonas	1000:1010	arg1	15894T					1032:1037	Xylanimonas cellulosilytica DSM 15894T	1000:1037	Xylanimonas cellulosilytica DSM 15894T	1000:1037	The ANI values of strain FW10M-9T with the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T and Xylanimonas cellulosilytica DSM 15894T was 81.5%, 81.2%, 81.0% and 84.1%, respectively, and the dDDH values estimated by GGDC was 24.3%, 24.3%, 29.3% and 28.1%, respectively.
32306134	4	67	theme	separate	409:416	arg1	branch					418:423	a separate branch	407:423	a separate branch with Lactococcus allomyrinae 1JSPR-7T	407:461	16S rRNA and rpoB gene sequences showed that strain 2DFWM-2T formed a separate branch with Lactococcus allomyrinae 1JSPR-7T in the genus Lactococcus, adjacent to a group of Lactococcus lactis subspecies.
32306134	8	68	theme	genus	1296:1300	arg1	Lactococcus					1302:1312	the genus Lactococcus	1292:1312	the genus Lactococcus	1292:1312	On the basis of the phenotypic and genotypic data, it is proposed that strain 2DFWM-2T represents a novel species of the genus Lactococcus, for which the name Lactococcus protaetiae sp.
32306134	6	69	theme	strain	701:706	arg1	FW10M-9T					708:715	strain FW10M-9T	701:715	strain FW10M-9T	701:715	Based on the 16S rRNA gene sequence analysis, strain FW10M-9T was classified into the genus Xylanimonas revealing 96.9-98.5% sequence similarities with the Xylanimonas species.
32306134	10	70	theme	Xylanimonas	1536:1546	arg1	sp					1559:1560	the name Xylanimonas protaetiae sp	1527:1560	the name Xylanimonas protaetiae sp	1527:1560	And, strain FW10M-9T represents a novel species of the genus Xylanimonas, for which the name Xylanimonas protaetiae sp.
32306134	6	71	theme	96.9-98.5	769:777	arg1	%					778:778	%	778:778	%	778:778	Based on the 16S rRNA gene sequence analysis, strain FW10M-9T was classified into the genus Xylanimonas revealing 96.9-98.5% sequence similarities with the Xylanimonas species.
32306134	8	72	theme	phenotypic	1195:1204	arg1	data					1220:1223	the phenotypic and genotypic data	1191:1223	the phenotypic and genotypic data	1191:1223	On the basis of the phenotypic and genotypic data, it is proposed that strain 2DFWM-2T represents a novel species of the genus Lactococcus, for which the name Lactococcus protaetiae sp.
32306134	3	73	theme	bacterial	137:145	arg1	strains					147:153	Two bacterial strains	133:153	Two bacterial strains	133:153	Two bacterial strains, designated 2DFWM-2T and FW10M-9T, were isolated from gut of larva of Protaetia brevitarsis seulensis grown at the National Institute of Agricultural Sciences, Wanju-gun, South Korea.
32306134	8	74	theme	genotypic	1210:1218	arg1	data					1220:1223	the phenotypic and genotypic data	1191:1223	the phenotypic and genotypic data	1191:1223	On the basis of the phenotypic and genotypic data, it is proposed that strain 2DFWM-2T represents a novel species of the genus Lactococcus, for which the name Lactococcus protaetiae sp.
32306134	2	75	theme	brevitarsis	110:120	arg1	seulensis					122:130	Protaetia brevitarsis seulensis	100:130	Protaetia brevitarsis seulensis	100:130	nov., isolated from gut of larvae of Protaetia brevitarsis seulensis.
32306134	1	76	theme	protaetiae	48:57	arg1	sp					59:60	protaetiae sp	48:60	protaetiae sp	48:60	nov. and Xylanimonas protaetiae sp.
32306134	9	77	theme	type	1387:1390	arg1	2DFWM-2T					1402:1409	2DFWM-2T	1402:1409	2DFWM-2T (= KACC 19320T = NBRC 113069T)	1402:1440	nov. is proposed, and the type strain is 2DFWM-2T (= KACC 19320T = NBRC 113069T).
32306134	9	77	theme	type	1387:1390	arg1	strain					1392:1397	the type strain	1383:1397	the type strain	1383:1397	nov. is proposed, and the type strain is 2DFWM-2T (= KACC 19320T = NBRC 113069T).
32306134	7	78	theme	FW10M-9T	857:864	arg1	%					1047:1047	81.5%	1043:1047	81.5%	1043:1047	The ANI values of strain FW10M-9T with the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T and Xylanimonas cellulosilytica DSM 15894T was 81.5%, 81.2%, 81.0% and 84.1%, respectively, and the dDDH values estimated by GGDC was 24.3%, 24.3%, 29.3% and 28.1%, respectively.
32306134	7	78	theme	FW10M-9T	857:864	arg1	values					840:845	The ANI values	832:845	The ANI values of strain FW10M-9T with the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T and Xylanimonas cellulosilytica DSM 15894T	832:1037	The ANI values of strain FW10M-9T with the closely species Xylanimonas pachnodae NBRC 107786T, Xylanimonas allomyrinae 2JSPR-7T, Isoptericola variabilis JCM 11754T and Xylanimonas cellulosilytica DSM 15894T was 81.5%, 81.2%, 81.0% and 84.1%, respectively, and the dDDH values estimated by GGDC was 24.3%, 24.3%, 29.3% and 28.1%, respectively.
32100673	2	0	theme	irregular	95:103	arg1	bacterium					116:124	A Gram-variable, aerobic, motile and irregular rod-shaped bacterium	58:124	A Gram-variable, aerobic, motile and irregular rod-shaped bacterium	58:124	A Gram-variable, aerobic, motile and irregular rod-shaped bacterium, designated HB172195T, was isolated from a mangrove sediment sample collected from Bamen Bay mangrove forest, China.
32100673	5	1	theme	respiratory	526:536	arg1	quinone					538:544	the sole respiratory quinone	517:544	the sole respiratory quinone	517:544	Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-7 and the cell-wall peptidoglycan was meso-diaminopimelic acid.
32100673	5	1	theme	respiratory	526:536	arg1	MK-7					550:553	MK-7	550:553	MK-7	550:553	Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-7 and the cell-wall peptidoglycan was meso-diaminopimelic acid.
32100673	8	2	theme	G+C	873:875	arg1	%					897:897	40.9 mol%	889:897	40.9 mol%	889:897	The genomic DNA G+C content was 40.9 mol%.
32100673	8	2	theme	G+C	873:875	arg1	content					877:883	The genomic DNA G+C content	857:883	The genomic DNA G+C content	857:883	The genomic DNA G+C content was 40.9 mol%.
32100673	12	3	theme	type	1295:1298	arg1	strain					1300:1305	The type strain	1291:1305	The type strain	1291:1305	The type strain is HB172195T (=CGMCC 1.16730T=JCM 33411T).
32100673	12	3	theme	type	1295:1298	arg1	HB172195T					1310:1318	HB172195T	1310:1318	HB172195T (=CGMCC 1.16730T=JCM 33411T)	1310:1347	The type strain is HB172195T (=CGMCC 1.16730T=JCM 33411T).
32100673	9	4	theme	Bacillus	1081:1088	arg1	076093T					1108:1114	Bacillus haemicentroti JSM 076093T	1081:1114	Bacillus haemicentroti JSM 076093T (95.5 %)	1081:1123	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was closely related to Bacillus hwajinpoensis SW-72T (96.3%), Bacillus algicola KMM 3737T (96.2 %) and Bacillus haemicentroti JSM 076093T (95.5 %).
32100673	9	4	theme	Bacillus	1081:1088	arg1	%					1122:1122	95.5 %	1117:1122	95.5 %	1117:1122	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was closely related to Bacillus hwajinpoensis SW-72T (96.3%), Bacillus algicola KMM 3737T (96.2 %) and Bacillus haemicentroti JSM 076093T (95.5 %).
32100673	4	5	theme	w/v	450:452	arg1	optimum					461:467	optimum	461:467	optimum	461:467	Strain HB172195T was found to grow at 15-50 °C (optimum, 25-40 °C), pH 5.0-9.0 (optimum, pH 7.0) and in 1.0-11.0 % (w/v) NaCl (optimum, 3-6 %).
32100673	4	5	theme	w/v	450:452	arg1	NaCl					455:458	1.0-11.0 % (w/v) NaCl	438:458	1.0-11.0 % (w/v) NaCl (optimum, 3-6 %)	438:475	Strain HB172195T was found to grow at 15-50 °C (optimum, 25-40 °C), pH 5.0-9.0 (optimum, pH 7.0) and in 1.0-11.0 % (w/v) NaCl (optimum, 3-6 %).
32100673	10	6	theme	Bacillus	1254:1261	arg1	sp					1269:1270	the name Bacillus caeni sp	1245:1270	the name Bacillus caeni sp	1245:1270	Based on polyphasic taxonomic characterization, strain HB172195T is considered to represent a novel species, for which the name Bacillus caeni sp.
32100673	2	7	theme	motile	84:89	arg1	bacterium					116:124	A Gram-variable, aerobic, motile and irregular rod-shaped bacterium	58:124	A Gram-variable, aerobic, motile and irregular rod-shaped bacterium	58:124	A Gram-variable, aerobic, motile and irregular rod-shaped bacterium, designated HB172195T, was isolated from a mangrove sediment sample collected from Bamen Bay mangrove forest, China.
32100673	10	8	theme	name	1249:1252	arg1	sp					1269:1270	the name Bacillus caeni sp	1245:1270	the name Bacillus caeni sp	1245:1270	Based on polyphasic taxonomic characterization, strain HB172195T is considered to represent a novel species, for which the name Bacillus caeni sp.
32100673	1	9	theme	mangrove	39:46	arg1	sediment					48:55	mangrove sediment	39:55	mangrove sediment	39:55	nov., isolated from mangrove sediment.
32100673	9	10	theme	KMM	1058:1060	arg1	3737T					1062:1066	Bacillus algicola KMM 3737T	1040:1066	Bacillus algicola KMM 3737T (96.2 %)	1040:1075	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was closely related to Bacillus hwajinpoensis SW-72T (96.3%), Bacillus algicola KMM 3737T (96.2 %) and Bacillus haemicentroti JSM 076093T (95.5 %).
32100673	9	10	theme	KMM	1058:1060	arg1	%					1074:1074	96.2 %	1069:1074	96.2 %	1069:1074	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was closely related to Bacillus hwajinpoensis SW-72T (96.3%), Bacillus algicola KMM 3737T (96.2 %) and Bacillus haemicentroti JSM 076093T (95.5 %).
32100673	8	11	theme	DNA	869:871	arg1	%					897:897	40.9 mol%	889:897	40.9 mol%	889:897	The genomic DNA G+C content was 40.9 mol%.
32100673	8	11	theme	DNA	869:871	arg1	content					877:883	The genomic DNA G+C content	857:883	The genomic DNA G+C content	857:883	The genomic DNA G+C content was 40.9 mol%.
32100673	5	12	theme	Chemotaxonomic	478:491	arg1	analysis					493:500	Chemotaxonomic analysis	478:500	Chemotaxonomic analysis	478:500	Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-7 and the cell-wall peptidoglycan was meso-diaminopimelic acid.
32100673	8	13	theme	mol	894:896	arg1	%					897:897	40.9 mol%	889:897	40.9 mol%	889:897	The genomic DNA G+C content was 40.9 mol%.
32100673	8	13	theme	mol	894:896	arg1	content					877:883	The genomic DNA G+C content	857:883	The genomic DNA G+C content	857:883	The genomic DNA G+C content was 40.9 mol%.
32100673	10	14	theme	taxonomic	1146:1154	arg1	characterization					1156:1171	polyphasic taxonomic characterization	1135:1171	polyphasic taxonomic characterization	1135:1171	Based on polyphasic taxonomic characterization, strain HB172195T is considered to represent a novel species, for which the name Bacillus caeni sp.
32100673	7	15	theme	major	720:724	arg1	lipids					732:737	The major polar lipids	716:737	The major polar lipids	716:737	The major polar lipids consisted of phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol and an unidentified phospholipid.
32100673	0	16	theme	caeni	9:13	arg1	sp					15:16	Bacillus caeni sp	0:16	Bacillus caeni sp.	0:17	Bacillus caeni sp.
32100673	10	17	theme	novel	1220:1224	arg1	species					1226:1232	a novel species	1218:1232	a novel species	1218:1232	Based on polyphasic taxonomic characterization, strain HB172195T is considered to represent a novel species, for which the name Bacillus caeni sp.
32100673	7	18	theme	polar	726:730	arg1	lipids					732:737	The major polar lipids	716:737	The major polar lipids	716:737	The major polar lipids consisted of phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol and an unidentified phospholipid.
32100673	0	19	theme	Bacillus	0:7	arg1	sp					15:16	Bacillus caeni sp	0:16	Bacillus caeni sp.	0:17	Bacillus caeni sp.
32100673	5	20	theme	sole	521:524	arg1	quinone					538:544	the sole respiratory quinone	517:544	the sole respiratory quinone	517:544	Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-7 and the cell-wall peptidoglycan was meso-diaminopimelic acid.
32100673	5	20	theme	sole	521:524	arg1	MK-7					550:553	MK-7	550:553	MK-7	550:553	Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-7 and the cell-wall peptidoglycan was meso-diaminopimelic acid.
32100673	7	21	theme	unidentified	830:841	arg1	phospholipid					843:854	an unidentified phospholipid	827:854	an unidentified phospholipid	827:854	The major polar lipids consisted of phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol and an unidentified phospholipid.
32100673	9	22	theme	Bacillus	1040:1047	arg1	3737T					1062:1066	Bacillus algicola KMM 3737T	1040:1066	Bacillus algicola KMM 3737T (96.2 %)	1040:1075	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was closely related to Bacillus hwajinpoensis SW-72T (96.3%), Bacillus algicola KMM 3737T (96.2 %) and Bacillus haemicentroti JSM 076093T (95.5 %).
32100673	9	22	theme	Bacillus	1040:1047	arg1	%					1074:1074	96.2 %	1069:1074	96.2 %	1069:1074	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was closely related to Bacillus hwajinpoensis SW-72T (96.3%), Bacillus algicola KMM 3737T (96.2 %) and Bacillus haemicentroti JSM 076093T (95.5 %).
32100673	4	23	dep	optimum	414:420	arg1	pH					423:424	pH 7.0	423:428	pH 7.0	423:428	Strain HB172195T was found to grow at 15-50 °C (optimum, 25-40 °C), pH 5.0-9.0 (optimum, pH 7.0) and in 1.0-11.0 % (w/v) NaCl (optimum, 3-6 %).
32100673	10	24	theme	caeni	1263:1267	arg1	sp					1269:1270	the name Bacillus caeni sp	1245:1270	the name Bacillus caeni sp	1245:1270	Based on polyphasic taxonomic characterization, strain HB172195T is considered to represent a novel species, for which the name Bacillus caeni sp.
32100673	6	25	dep	0	690:690	arg1	0					673:673	0	673:673	0	673:673	The predominant cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1ω7c alcohol.
32100673	2	26	attach	isolated	153:160	arg2	bacterium					116:124	A Gram-variable, aerobic, motile and irregular rod-shaped bacterium	58:124	A Gram-variable, aerobic, motile and irregular rod-shaped bacterium	58:124	A Gram-variable, aerobic, motile and irregular rod-shaped bacterium, designated HB172195T, was isolated from a mangrove sediment sample collected from Bamen Bay mangrove forest, China.
32100673	2	26	attach	isolated	153:160	arg1	sample					187:192	a mangrove sediment sample	167:192	a mangrove sediment sample collected from Bamen Bay mangrove forest, China	167:240	A Gram-variable, aerobic, motile and irregular rod-shaped bacterium, designated HB172195T, was isolated from a mangrove sediment sample collected from Bamen Bay mangrove forest, China.
32100673	8	27	theme	genomic	861:867	arg1	%					897:897	40.9 mol%	889:897	40.9 mol%	889:897	The genomic DNA G+C content was 40.9 mol%.
32100673	8	27	theme	genomic	861:867	arg1	content					877:883	The genomic DNA G+C content	857:883	The genomic DNA G+C content	857:883	The genomic DNA G+C content was 40.9 mol%.
32100673	5	28	theme	meso-diaminopimelic	591:609	arg1	peptidoglycan					573:585	the cell-wall peptidoglycan	559:585	the cell-wall peptidoglycan	559:585	Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-7 and the cell-wall peptidoglycan was meso-diaminopimelic acid.
32100673	5	28	theme	meso-diaminopimelic	591:609	arg1	acid					611:614	meso-diaminopimelic acid	591:614	meso-diaminopimelic acid	591:614	Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-7 and the cell-wall peptidoglycan was meso-diaminopimelic acid.
32100673	9	29	theme	JSM	1104:1106	arg1	076093T					1108:1114	Bacillus haemicentroti JSM 076093T	1081:1114	Bacillus haemicentroti JSM 076093T (95.5 %)	1081:1123	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was closely related to Bacillus hwajinpoensis SW-72T (96.3%), Bacillus algicola KMM 3737T (96.2 %) and Bacillus haemicentroti JSM 076093T (95.5 %).
32100673	9	29	theme	JSM	1104:1106	arg1	%					1122:1122	95.5 %	1117:1122	95.5 %	1117:1122	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was closely related to Bacillus hwajinpoensis SW-72T (96.3%), Bacillus algicola KMM 3737T (96.2 %) and Bacillus haemicentroti JSM 076093T (95.5 %).
32100673	10	30	theme	strain	1174:1179	arg1	HB172195T					1181:1189	strain HB172195T	1174:1189	strain HB172195T	1174:1189	Based on polyphasic taxonomic characterization, strain HB172195T is considered to represent a novel species, for which the name Bacillus caeni sp.
32100673	3	31	dep	catalase	302:309	arg1	reduction					323:331	reduction	323:331	reduction	323:331	Cells of the strain were oxidase-negative but positive for catalase and nitrate reduction.
32100673	9	32	dep	Bacillus	1040:1047	arg1	algicola					1049:1056	algicola	1049:1056	algicola	1049:1056	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was closely related to Bacillus hwajinpoensis SW-72T (96.3%), Bacillus algicola KMM 3737T (96.2 %) and Bacillus haemicentroti JSM 076093T (95.5 %).
32100673	9	33	theme	Phylogenetic	900:911	arg1	analysis					913:920	Phylogenetic analysis	900:920	Phylogenetic analysis based on 16S rRNA gene sequences	900:953	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was closely related to Bacillus hwajinpoensis SW-72T (96.3%), Bacillus algicola KMM 3737T (96.2 %) and Bacillus haemicentroti JSM 076093T (95.5 %).
32100673	4	34	theme	1.0-11.0 	438:446	arg1	optimum					461:467	optimum	461:467	optimum	461:467	Strain HB172195T was found to grow at 15-50 °C (optimum, 25-40 °C), pH 5.0-9.0 (optimum, pH 7.0) and in 1.0-11.0 % (w/v) NaCl (optimum, 3-6 %).
32100673	4	34	theme	1.0-11.0 	438:446	arg1	NaCl					455:458	1.0-11.0 % (w/v) NaCl	438:458	1.0-11.0 % (w/v) NaCl (optimum, 3-6 %)	438:475	Strain HB172195T was found to grow at 15-50 °C (optimum, 25-40 °C), pH 5.0-9.0 (optimum, pH 7.0) and in 1.0-11.0 % (w/v) NaCl (optimum, 3-6 %).
32100673	4	35	theme	%	447:447	arg1	optimum					461:467	optimum	461:467	optimum	461:467	Strain HB172195T was found to grow at 15-50 °C (optimum, 25-40 °C), pH 5.0-9.0 (optimum, pH 7.0) and in 1.0-11.0 % (w/v) NaCl (optimum, 3-6 %).
32100673	4	35	theme	%	447:447	arg1	NaCl					455:458	1.0-11.0 % (w/v) NaCl	438:458	1.0-11.0 % (w/v) NaCl (optimum, 3-6 %)	438:475	Strain HB172195T was found to grow at 15-50 °C (optimum, 25-40 °C), pH 5.0-9.0 (optimum, pH 7.0) and in 1.0-11.0 % (w/v) NaCl (optimum, 3-6 %).
32100673	2	36	theme	aerobic	75:81	arg1	bacterium					116:124	A Gram-variable, aerobic, motile and irregular rod-shaped bacterium	58:124	A Gram-variable, aerobic, motile and irregular rod-shaped bacterium	58:124	A Gram-variable, aerobic, motile and irregular rod-shaped bacterium, designated HB172195T, was isolated from a mangrove sediment sample collected from Bamen Bay mangrove forest, China.
32100673	4	37	dep	optimum	461:467	arg1	%					474:474	3-6 %	470:474	3-6 %	470:474	Strain HB172195T was found to grow at 15-50 °C (optimum, 25-40 °C), pH 5.0-9.0 (optimum, pH 7.0) and in 1.0-11.0 % (w/v) NaCl (optimum, 3-6 %).
32100673	5	38	theme	cell-wall	563:571	arg1	peptidoglycan					573:585	the cell-wall peptidoglycan	559:585	the cell-wall peptidoglycan	559:585	Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-7 and the cell-wall peptidoglycan was meso-diaminopimelic acid.
32100673	5	38	theme	cell-wall	563:571	arg1	acid					611:614	meso-diaminopimelic acid	591:614	meso-diaminopimelic acid	591:614	Chemotaxonomic analysis indicated that the sole respiratory quinone was MK-7 and the cell-wall peptidoglycan was meso-diaminopimelic acid.
32100673	9	39	dep	Bacillus	1001:1008	arg1	hwajinpoensis					1010:1022	hwajinpoensis	1010:1022	hwajinpoensis	1010:1022	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was closely related to Bacillus hwajinpoensis SW-72T (96.3%), Bacillus algicola KMM 3737T (96.2 %) and Bacillus haemicentroti JSM 076093T (95.5 %).
32100673	12	40	theme	=CGMCC	1321:1326	arg1	33411T					1341:1346	=CGMCC 1.16730T=JCM 33411T	1321:1346	=CGMCC 1.16730T=JCM 33411T	1321:1346	The type strain is HB172195T (=CGMCC 1.16730T=JCM 33411T).
32100673	12	40	theme	=CGMCC	1321:1326	arg1	HB172195T					1310:1318	HB172195T	1310:1318	HB172195T (=CGMCC 1.16730T=JCM 33411T)	1310:1347	The type strain is HB172195T (=CGMCC 1.16730T=JCM 33411T).
32100673	4	41	theme	Strain	334:339	arg1	HB172195T					341:349	Strain HB172195T	334:349	Strain HB172195T	334:349	Strain HB172195T was found to grow at 15-50 °C (optimum, 25-40 °C), pH 5.0-9.0 (optimum, pH 7.0) and in 1.0-11.0 % (w/v) NaCl (optimum, 3-6 %).
32100673	2	42	theme	Gram-variable	60:72	arg1	bacterium					116:124	A Gram-variable, aerobic, motile and irregular rod-shaped bacterium	58:124	A Gram-variable, aerobic, motile and irregular rod-shaped bacterium	58:124	A Gram-variable, aerobic, motile and irregular rod-shaped bacterium, designated HB172195T, was isolated from a mangrove sediment sample collected from Bamen Bay mangrove forest, China.
32100673	3	43	theme	strain	256:261	arg1	Cells					243:247	Cells	243:247	Cells of the strain	243:261	Cells of the strain were oxidase-negative but positive for catalase and nitrate reduction.
32100673	2	44	theme	sediment	178:185	arg1	sample					187:192	a mangrove sediment sample	167:192	a mangrove sediment sample collected from Bamen Bay mangrove forest, China	167:240	A Gram-variable, aerobic, motile and irregular rod-shaped bacterium, designated HB172195T, was isolated from a mangrove sediment sample collected from Bamen Bay mangrove forest, China.
32100673	9	45	theme	16S	931:933	arg1	sequences					945:953	16S rRNA gene sequences	931:953	16S rRNA gene sequences	931:953	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was closely related to Bacillus hwajinpoensis SW-72T (96.3%), Bacillus algicola KMM 3737T (96.2 %) and Bacillus haemicentroti JSM 076093T (95.5 %).
32100673	2	46	theme	mangrove	169:176	arg1	sample					187:192	a mangrove sediment sample	167:192	a mangrove sediment sample collected from Bamen Bay mangrove forest, China	167:240	A Gram-variable, aerobic, motile and irregular rod-shaped bacterium, designated HB172195T, was isolated from a mangrove sediment sample collected from Bamen Bay mangrove forest, China.
32100673	6	47	theme	fatty	642:646	arg1	alcohol					707:713	anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1ω7c alcohol	659:713	anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1ω7c alcohol	659:713	The predominant cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1ω7c alcohol.
32100673	6	47	theme	fatty	642:646	arg1	acids					648:652	The predominant cellular fatty acids	617:652	The predominant cellular fatty acids	617:652	The predominant cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1ω7c alcohol.
32100673	9	48	theme	Bacillus	1001:1008	arg1	%					1036:1036	96.3%	1032:1036	96.3%	1032:1036	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was closely related to Bacillus hwajinpoensis SW-72T (96.3%), Bacillus algicola KMM 3737T (96.2 %) and Bacillus haemicentroti JSM 076093T (95.5 %).
32100673	9	48	theme	Bacillus	1001:1008	arg1	SW-72T					1024:1029	Bacillus hwajinpoensis SW-72T	1001:1029	Bacillus hwajinpoensis SW-72T (96.3%)	1001:1037	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was closely related to Bacillus hwajinpoensis SW-72T (96.3%), Bacillus algicola KMM 3737T (96.2 %) and Bacillus haemicentroti JSM 076093T (95.5 %).
32100673	9	49	theme	rRNA	935:938	arg1	sequences					945:953	16S rRNA gene sequences	931:953	16S rRNA gene sequences	931:953	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was closely related to Bacillus hwajinpoensis SW-72T (96.3%), Bacillus algicola KMM 3737T (96.2 %) and Bacillus haemicentroti JSM 076093T (95.5 %).
32100673	10	50	theme	polyphasic	1135:1144	arg1	characterization					1156:1171	polyphasic taxonomic characterization	1135:1171	polyphasic taxonomic characterization	1135:1171	Based on polyphasic taxonomic characterization, strain HB172195T is considered to represent a novel species, for which the name Bacillus caeni sp.
32100673	12	51	theme	1.16730T=JCM	1328:1339	arg1	33411T					1341:1346	=CGMCC 1.16730T=JCM 33411T	1321:1346	=CGMCC 1.16730T=JCM 33411T	1321:1346	The type strain is HB172195T (=CGMCC 1.16730T=JCM 33411T).
32100673	12	51	theme	1.16730T=JCM	1328:1339	arg1	HB172195T					1310:1318	HB172195T	1310:1318	HB172195T (=CGMCC 1.16730T=JCM 33411T)	1310:1347	The type strain is HB172195T (=CGMCC 1.16730T=JCM 33411T).
32100673	6	52	theme	cellular	633:640	arg1	alcohol					707:713	anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1ω7c alcohol	659:713	anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1ω7c alcohol	659:713	The predominant cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1ω7c alcohol.
32100673	6	52	theme	cellular	633:640	arg1	acids					648:652	The predominant cellular fatty acids	617:652	The predominant cellular fatty acids	617:652	The predominant cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1ω7c alcohol.
32100673	9	53	theme	gene	940:943	arg1	sequences					945:953	16S rRNA gene sequences	931:953	16S rRNA gene sequences	931:953	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was closely related to Bacillus hwajinpoensis SW-72T (96.3%), Bacillus algicola KMM 3737T (96.2 %) and Bacillus haemicentroti JSM 076093T (95.5 %).
32100673	4	54	dep	optimum	382:388	arg1	°C					397:398	25-40 °C	391:398	25-40 °C	391:398	Strain HB172195T was found to grow at 15-50 °C (optimum, 25-40 °C), pH 5.0-9.0 (optimum, pH 7.0) and in 1.0-11.0 % (w/v) NaCl (optimum, 3-6 %).
32100673	2	55	theme	rod-shaped	105:114	arg1	bacterium					116:124	A Gram-variable, aerobic, motile and irregular rod-shaped bacterium	58:124	A Gram-variable, aerobic, motile and irregular rod-shaped bacterium	58:124	A Gram-variable, aerobic, motile and irregular rod-shaped bacterium, designated HB172195T, was isolated from a mangrove sediment sample collected from Bamen Bay mangrove forest, China.
32100673	6	56	theme	predominant	621:631	arg1	alcohol					707:713	anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1ω7c alcohol	659:713	anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1ω7c alcohol	659:713	The predominant cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1ω7c alcohol.
32100673	6	56	theme	predominant	621:631	arg1	acids					648:652	The predominant cellular fatty acids	617:652	The predominant cellular fatty acids	617:652	The predominant cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 1ω7c alcohol.
32100673	9	57	dep	Bacillus	1081:1088	arg1	haemicentroti					1090:1102	haemicentroti	1090:1102	haemicentroti	1090:1102	Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain was closely related to Bacillus hwajinpoensis SW-72T (96.3%), Bacillus algicola KMM 3737T (96.2 %) and Bacillus haemicentroti JSM 076093T (95.5 %).
31710581	11	0	theme	polar	1314:1318	arg1	diphosphatidylglycerol					1332:1353	diphosphatidylglycerol	1332:1353	diphosphatidylglycerol	1332:1353	The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and unidentified phospholipids.
31710581	11	0	theme	polar	1314:1318	arg1	lipids					1320:1325	The predominant polar lipids	1298:1325	The predominant polar lipids	1298:1325	The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and unidentified phospholipids.
31710581	13	1	theme	analysis	1574:1581	arg1	results					1550:1556	the results	1546:1556	the results of phylogenetic analysis and phenotypic and chemotaxonomic characteristics	1546:1631	On the basis of the results of phylogenetic analysis and phenotypic and chemotaxonomic characteristics, strain N5BH11T represents a novel species of the genus Nakamurella, for which the name Nakamurella flava sp.
31710581	13	2	theme	novel	1662:1666	arg1	species					1668:1674	a novel species	1660:1674	a novel species	1660:1674	On the basis of the results of phylogenetic analysis and phenotypic and chemotaxonomic characteristics, strain N5BH11T represents a novel species of the genus Nakamurella, for which the name Nakamurella flava sp.
31710581	6	3	theme	gene	704:707	arg1	similarity					718:727	the highest 16S rRNA gene sequence similarity	683:727	the highest 16S rRNA gene sequence similarity to Nakamurella flavida DS-52T (98.1 %)	683:766	Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain N5BH11T belonged to the genus Nakamurella and had the highest 16S rRNA gene sequence similarity to Nakamurella flavida DS-52T (98.1 %).
31710581	10	4	theme	cell-wall	1192:1200	arg1	peptidoglycan					1202:1214	The cell-wall peptidoglycan	1188:1214	The cell-wall peptidoglycan	1188:1214	The cell-wall peptidoglycan contained meso-diaminopimelic acid, and MK-8(H4) was the predominant menaquinone.
31710581	9	5	theme	type	1081:1084	arg1	strains					1086:1092	the type strains	1077:1092	the type strains of N. panacisegetis, N. multipartita and N. lactea	1077:1143	The estimated DDH values between strain N5BH11T and the type strains of N. panacisegetis, N. multipartita and N. lactea were 20.3%, 21.4 and 20.2 %, respectively.
31710581	9	5	theme	type	1081:1084	arg1	%					1154:1154	20.3%	1150:1154	20.3%	1150:1154	The estimated DDH values between strain N5BH11T and the type strains of N. panacisegetis, N. multipartita and N. lactea were 20.3%, 21.4 and 20.2 %, respectively.
31710581	13	6	theme	chemotaxonomic	1602:1615	arg1	characteristics					1617:1631	phenotypic and chemotaxonomic characteristics	1587:1631	phenotypic and chemotaxonomic characteristics	1587:1631	On the basis of the results of phylogenetic analysis and phenotypic and chemotaxonomic characteristics, strain N5BH11T represents a novel species of the genus Nakamurella, for which the name Nakamurella flava sp.
31710581	2	7	theme	Gram-stain-positive	103:121	arg1	actinobacterium					166:180	A Gram-stain-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium	101:180	A Gram-stain-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium	101:180	A Gram-stain-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain N5BH11T, was isolated from a surface-sterilized sample of Mentha haplocalyx Briq.
31710581	9	8	theme	DDH	1039:1041	arg1	values					1043:1048	The estimated DDH values	1025:1048	The estimated DDH values between strain N5BH11T	1025:1071	The estimated DDH values between strain N5BH11T and the type strains of N. panacisegetis, N. multipartita and N. lactea were 20.3%, 21.4 and 20.2 %, respectively.
31710581	9	8	theme	DDH	1039:1041	arg1	%					1154:1154	20.3%	1150:1154	20.3%	1150:1154	The estimated DDH values between strain N5BH11T and the type strains of N. panacisegetis, N. multipartita and N. lactea were 20.3%, 21.4 and 20.2 %, respectively.
31710581	13	9	theme	flava	1733:1737	arg1	sp					1739:1740	the name Nakamurella flava sp	1712:1740	the name Nakamurella flava sp	1712:1740	On the basis of the results of phylogenetic analysis and phenotypic and chemotaxonomic characteristics, strain N5BH11T represents a novel species of the genus Nakamurella, for which the name Nakamurella flava sp.
31710581	6	10	theme	16S	588:590	arg1	rRNA					592:595	16S rRNA	588:595	16S rRNA gene sequence	588:609	Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain N5BH11T belonged to the genus Nakamurella and had the highest 16S rRNA gene sequence similarity to Nakamurella flavida DS-52T (98.1 %).
31710581	8	11	theme	multipartita	947:958	arg1	strains					897:903	the type strains	888:903	the type strains of Nakamurella panacisegetis, Nakamurella multipartita and Nakamurella lactea	888:981	The average nucleotide identity values between strain N5BH11T and the type strains of Nakamurella panacisegetis, Nakamurella multipartita and Nakamurella lactea were 74.0, 76.5 and 73.6 %, respectively.
31710581	8	11	theme	multipartita	947:958	arg1	%					1008:1008	74.0, 76.5 and 73.6 %	988:1008	74.0, 76.5 and 73.6 %	988:1008	The average nucleotide identity values between strain N5BH11T and the type strains of Nakamurella panacisegetis, Nakamurella multipartita and Nakamurella lactea were 74.0, 76.5 and 73.6 %, respectively.
31710581	8	11	theme	multipartita	947:958	arg1	values					854:859	The average nucleotide identity values	822:859	The average nucleotide identity values between strain N5BH11T	822:882	The average nucleotide identity values between strain N5BH11T and the type strains of Nakamurella panacisegetis, Nakamurella multipartita and Nakamurella lactea were 74.0, 76.5 and 73.6 %, respectively.
31710581	1	12	theme	endophytic	36:45	arg1	actinobacterium					47:61	a novel endophytic actinobacterium	28:61	a novel endophytic actinobacterium	28:61	nov., a novel endophytic actinobacterium isolated from Mentha haplocalyx Briq.
31710581	1	12	theme	endophytic	36:45	arg1	nov.					22:25	nov.	22:25	nov.	22:25	nov., a novel endophytic actinobacterium isolated from Mentha haplocalyx Briq.
31710581	9	13	theme	estimated	1029:1037	arg1	values					1043:1048	The estimated DDH values	1025:1048	The estimated DDH values between strain N5BH11T	1025:1071	The estimated DDH values between strain N5BH11T and the type strains of N. panacisegetis, N. multipartita and N. lactea were 20.3%, 21.4 and 20.2 %, respectively.
31710581	9	13	theme	estimated	1029:1037	arg1	%					1154:1154	20.3%	1150:1154	20.3%	1150:1154	The estimated DDH values between strain N5BH11T and the type strains of N. panacisegetis, N. multipartita and N. lactea were 20.3%, 21.4 and 20.2 %, respectively.
31710581	13	14	theme	Nakamurella	1689:1699	arg1	species					1668:1674	a novel species	1660:1674	a novel species	1660:1674	On the basis of the results of phylogenetic analysis and phenotypic and chemotaxonomic characteristics, strain N5BH11T represents a novel species of the genus Nakamurella, for which the name Nakamurella flava sp.
31710581	8	15	theme	lactea	976:981	arg1	strains					897:903	the type strains	888:903	the type strains of Nakamurella panacisegetis, Nakamurella multipartita and Nakamurella lactea	888:981	The average nucleotide identity values between strain N5BH11T and the type strains of Nakamurella panacisegetis, Nakamurella multipartita and Nakamurella lactea were 74.0, 76.5 and 73.6 %, respectively.
31710581	8	15	theme	lactea	976:981	arg1	%					1008:1008	74.0, 76.5 and 73.6 %	988:1008	74.0, 76.5 and 73.6 %	988:1008	The average nucleotide identity values between strain N5BH11T and the type strains of Nakamurella panacisegetis, Nakamurella multipartita and Nakamurella lactea were 74.0, 76.5 and 73.6 %, respectively.
31710581	8	15	theme	lactea	976:981	arg1	values					854:859	The average nucleotide identity values	822:859	The average nucleotide identity values between strain N5BH11T	822:882	The average nucleotide identity values between strain N5BH11T and the type strains of Nakamurella panacisegetis, Nakamurella multipartita and Nakamurella lactea were 74.0, 76.5 and 73.6 %, respectively.
31710581	12	16	theme	major	1453:1457	arg1	iso-C15 					1476:1483	iso-C15 	1476:1483	iso-C15 	1476:1483	The major fatty acids were iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and C16 : 1ω7c.
31710581	12	16	theme	major	1453:1457	arg1	acids					1465:1469	The major fatty acids	1449:1469	The major fatty acids	1449:1469	The major fatty acids were iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and C16 : 1ω7c.
31710581	6	17	theme	rRNA	699:702	arg1	similarity					718:727	the highest 16S rRNA gene sequence similarity	683:727	the highest 16S rRNA gene sequence similarity to Nakamurella flavida DS-52T (98.1 %)	683:766	Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain N5BH11T belonged to the genus Nakamurella and had the highest 16S rRNA gene sequence similarity to Nakamurella flavida DS-52T (98.1 %).
31710581	9	18	theme	strain	1058:1063	arg1	N5BH11T					1065:1071	strain N5BH11T	1058:1071	strain N5BH11T	1058:1071	The estimated DDH values between strain N5BH11T and the type strains of N. panacisegetis, N. multipartita and N. lactea were 20.3%, 21.4 and 20.2 %, respectively.
31710581	5	19	located	observed	527:534	arg1	media					543:547	the media	539:547	the media tested	539:554	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
31710581	5	19	located	observed	527:534	arg2	pigments					513:520	no diffusible pigments	499:520	no diffusible pigments	499:520	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
31710581	8	20	theme	nucleotide	834:843	arg1	%					1008:1008	74.0, 76.5 and 73.6 %	988:1008	74.0, 76.5 and 73.6 %	988:1008	The average nucleotide identity values between strain N5BH11T and the type strains of Nakamurella panacisegetis, Nakamurella multipartita and Nakamurella lactea were 74.0, 76.5 and 73.6 %, respectively.
31710581	8	20	theme	nucleotide	834:843	arg1	values					854:859	The average nucleotide identity values	822:859	The average nucleotide identity values between strain N5BH11T	822:882	The average nucleotide identity values between strain N5BH11T and the type strains of Nakamurella panacisegetis, Nakamurella multipartita and Nakamurella lactea were 74.0, 76.5 and 73.6 %, respectively.
31710581	13	21	theme	strain	1634:1639	arg1	N5BH11T					1641:1647	strain N5BH11T	1634:1647	strain N5BH11T	1634:1647	On the basis of the results of phylogenetic analysis and phenotypic and chemotaxonomic characteristics, strain N5BH11T represents a novel species of the genus Nakamurella, for which the name Nakamurella flava sp.
31710581	6	22	theme	16S	695:697	arg1	similarity					718:727	the highest 16S rRNA gene sequence similarity	683:727	the highest 16S rRNA gene sequence similarity to Nakamurella flavida DS-52T (98.1 %)	683:766	Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain N5BH11T belonged to the genus Nakamurella and had the highest 16S rRNA gene sequence similarity to Nakamurella flavida DS-52T (98.1 %).
31710581	13	23	theme	phenotypic	1587:1596	arg1	characteristics					1617:1631	phenotypic and chemotaxonomic characteristics	1587:1631	phenotypic and chemotaxonomic characteristics	1587:1631	On the basis of the results of phylogenetic analysis and phenotypic and chemotaxonomic characteristics, strain N5BH11T represents a novel species of the genus Nakamurella, for which the name Nakamurella flava sp.
31710581	3	24	dep	China	310:314	arg1	PR					307:308	Guizhou, PR China	298:314	PR	307:308	collected from Guizhou, PR China and tested by a polyphasic approach to determine its taxonomic position.
31710581	6	25	theme	rRNA	592:595	arg1	sequence					602:609	16S rRNA gene sequence	588:609	16S rRNA gene sequence	588:609	Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain N5BH11T belonged to the genus Nakamurella and had the highest 16S rRNA gene sequence similarity to Nakamurella flavida DS-52T (98.1 %).
31710581	6	26	theme	highest	687:693	arg1	similarity					718:727	the highest 16S rRNA gene sequence similarity	683:727	the highest 16S rRNA gene sequence similarity to Nakamurella flavida DS-52T (98.1 %)	683:766	Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain N5BH11T belonged to the genus Nakamurella and had the highest 16S rRNA gene sequence similarity to Nakamurella flavida DS-52T (98.1 %).
31710581	2	27	attach	isolated	214:221	arg2	actinobacterium					166:180	A Gram-stain-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium	101:180	A Gram-stain-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium	101:180	A Gram-stain-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain N5BH11T, was isolated from a surface-sterilized sample of Mentha haplocalyx Briq.
31710581	2	27	attach	isolated	214:221	arg1	sample					249:254	a surface-sterilized sample	228:254	a surface-sterilized sample of Mentha haplocalyx Briq	228:280	A Gram-stain-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain N5BH11T, was isolated from a surface-sterilized sample of Mentha haplocalyx Briq.
31710581	0	28	theme	flava	12:16	arg1	sp					18:19	Nakamurella flava sp	0:19	Nakamurella flava sp.	0:20	Nakamurella flava sp.
31710581	15	29	theme	=KCTC	1789:1793	arg1	N5BH11T					1780:1786	N5BH11T	1780:1786	N5BH11T (=KCTC 49196T=CGMCC 4.7524T)	1780:1815	The type strain is N5BH11T (=KCTC 49196T=CGMCC 4.7524T).
31710581	15	29	theme	=KCTC	1789:1793	arg1	4.7524T					1808:1814	=KCTC 49196T=CGMCC 4.7524T	1789:1814	=KCTC 49196T=CGMCC 4.7524T	1789:1814	The type strain is N5BH11T (=KCTC 49196T=CGMCC 4.7524T).
31710581	1	30	theme	Mentha	77:82	arg1	Briq					95:98	Mentha haplocalyx Briq	77:98	Mentha haplocalyx Briq	77:98	nov., a novel endophytic actinobacterium isolated from Mentha haplocalyx Briq.
31710581	8	31	theme	identity	845:852	arg1	%					1008:1008	74.0, 76.5 and 73.6 %	988:1008	74.0, 76.5 and 73.6 %	988:1008	The average nucleotide identity values between strain N5BH11T and the type strains of Nakamurella panacisegetis, Nakamurella multipartita and Nakamurella lactea were 74.0, 76.5 and 73.6 %, respectively.
31710581	8	31	theme	identity	845:852	arg1	values					854:859	The average nucleotide identity values	822:859	The average nucleotide identity values between strain N5BH11T	822:882	The average nucleotide identity values between strain N5BH11T and the type strains of Nakamurella panacisegetis, Nakamurella multipartita and Nakamurella lactea were 74.0, 76.5 and 73.6 %, respectively.
31710581	0	32	theme	Nakamurella	0:10	arg1	sp					18:19	Nakamurella flava sp	0:19	Nakamurella flava sp.	0:20	Nakamurella flava sp.
31710581	7	33	theme	G+C	777:779	arg1	%					819:819	71.6 mol%	811:819	71.6 mol%	811:819	The DNA G+C content of strain N5BH11T was 71.6 mol%.
31710581	7	33	theme	G+C	777:779	arg1	content					781:787	The DNA G+C content	769:787	The DNA G+C content of strain N5BH11T	769:805	The DNA G+C content of strain N5BH11T was 71.6 mol%.
31710581	6	34	contain	had	679:681	arg1	N5BH11T					633:639	strain N5BH11T	626:639	strain N5BH11T	626:639	Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain N5BH11T belonged to the genus Nakamurella and had the highest 16S rRNA gene sequence similarity to Nakamurella flavida DS-52T (98.1 %).
31710581	6	34	contain	had	679:681	arg2	similarity					718:727	the highest 16S rRNA gene sequence similarity	683:727	the highest 16S rRNA gene sequence similarity to Nakamurella flavida DS-52T (98.1 %)	683:766	Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain N5BH11T belonged to the genus Nakamurella and had the highest 16S rRNA gene sequence similarity to Nakamurella flavida DS-52T (98.1 %).
31710581	4	35	theme	Strain	389:394	arg1	N5BH11T					396:402	Strain N5BH11T	389:402	Strain N5BH11T	389:402	Strain N5BH11T grew optimally at 30 °C, pH 6.0-7.0.
31710581	1	36	theme	haplocalyx	84:93	arg1	Briq					95:98	Mentha haplocalyx Briq	77:98	Mentha haplocalyx Briq	77:98	nov., a novel endophytic actinobacterium isolated from Mentha haplocalyx Briq.
31710581	5	37	theme	aerial	463:468	arg1	mycelia					470:476	aerial mycelia	463:476	aerial mycelia	463:476	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
31710581	3	38	theme	taxonomic	369:377	arg1	position					379:386	its taxonomic position	365:386	its taxonomic position	365:386	collected from Guizhou, PR China and tested by a polyphasic approach to determine its taxonomic position.
31710581	9	39	theme	lactea	1138:1143	arg1	strains					1086:1092	the type strains	1077:1092	the type strains of N. panacisegetis, N. multipartita and N. lactea	1077:1143	The estimated DDH values between strain N5BH11T and the type strains of N. panacisegetis, N. multipartita and N. lactea were 20.3%, 21.4 and 20.2 %, respectively.
31710581	9	39	theme	lactea	1138:1143	arg1	values					1043:1048	The estimated DDH values	1025:1048	The estimated DDH values between strain N5BH11T	1025:1071	The estimated DDH values between strain N5BH11T and the type strains of N. panacisegetis, N. multipartita and N. lactea were 20.3%, 21.4 and 20.2 %, respectively.
31710581	9	39	theme	lactea	1138:1143	arg1	%					1154:1154	20.3%	1150:1154	20.3%	1150:1154	The estimated DDH values between strain N5BH11T and the type strains of N. panacisegetis, N. multipartita and N. lactea were 20.3%, 21.4 and 20.2 %, respectively.
31710581	15	40	theme	type	1765:1768	arg1	N5BH11T					1780:1786	N5BH11T	1780:1786	N5BH11T (=KCTC 49196T=CGMCC 4.7524T)	1780:1815	The type strain is N5BH11T (=KCTC 49196T=CGMCC 4.7524T).
31710581	15	40	theme	type	1765:1768	arg1	strain					1770:1775	The type strain	1761:1775	The type strain	1761:1775	The type strain is N5BH11T (=KCTC 49196T=CGMCC 4.7524T).
31710581	13	41	theme	Nakamurella	1721:1731	arg1	sp					1739:1740	the name Nakamurella flava sp	1712:1740	the name Nakamurella flava sp	1712:1740	On the basis of the results of phylogenetic analysis and phenotypic and chemotaxonomic characteristics, strain N5BH11T represents a novel species of the genus Nakamurella, for which the name Nakamurella flava sp.
31710581	3	42	theme	Guizhou	298:304	arg1	China					310:314	Guizhou, PR China	298:314	China	310:314	collected from Guizhou, PR China and tested by a polyphasic approach to determine its taxonomic position.
31710581	2	43	theme	strain	194:199	arg1	N5BH11T					201:207	strain N5BH11T	194:207	strain N5BH11T	194:207	A Gram-stain-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain N5BH11T, was isolated from a surface-sterilized sample of Mentha haplocalyx Briq.
31710581	7	44	theme	strain	792:797	arg1	N5BH11T					799:805	strain N5BH11T	792:805	strain N5BH11T	792:805	The DNA G+C content of strain N5BH11T was 71.6 mol%.
31710581	7	45	theme	N5BH11T	799:805	arg1	%					819:819	71.6 mol%	811:819	71.6 mol%	811:819	The DNA G+C content of strain N5BH11T was 71.6 mol%.
31710581	7	45	theme	N5BH11T	799:805	arg1	content					781:787	The DNA G+C content	769:787	The DNA G+C content of strain N5BH11T	769:805	The DNA G+C content of strain N5BH11T was 71.6 mol%.
31710581	13	46	theme	genus	1683:1687	arg1	Nakamurella					1689:1699	the genus Nakamurella	1679:1699	the genus Nakamurella	1679:1699	On the basis of the results of phylogenetic analysis and phenotypic and chemotaxonomic characteristics, strain N5BH11T represents a novel species of the genus Nakamurella, for which the name Nakamurella flava sp.
31710581	7	47	theme	DNA	773:775	arg1	%					819:819	71.6 mol%	811:819	71.6 mol%	811:819	The DNA G+C content of strain N5BH11T was 71.6 mol%.
31710581	7	47	theme	DNA	773:775	arg1	content					781:787	The DNA G+C content	769:787	The DNA G+C content of strain N5BH11T	769:805	The DNA G+C content of strain N5BH11T was 71.6 mol%.
31710581	13	48	theme	characteristics	1617:1631	arg1	results					1550:1556	the results	1546:1556	the results of phylogenetic analysis and phenotypic and chemotaxonomic characteristics	1546:1631	On the basis of the results of phylogenetic analysis and phenotypic and chemotaxonomic characteristics, strain N5BH11T represents a novel species of the genus Nakamurella, for which the name Nakamurella flava sp.
31710581	6	49	theme	genus	657:661	arg1	Nakamurella					663:673	the genus Nakamurella	653:673	the genus Nakamurella	653:673	Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain N5BH11T belonged to the genus Nakamurella and had the highest 16S rRNA gene sequence similarity to Nakamurella flavida DS-52T (98.1 %).
31710581	11	50	theme	predominant	1302:1312	arg1	diphosphatidylglycerol					1332:1353	diphosphatidylglycerol	1332:1353	diphosphatidylglycerol	1332:1353	The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and unidentified phospholipids.
31710581	11	50	theme	predominant	1302:1312	arg1	lipids					1320:1325	The predominant polar lipids	1298:1325	The predominant polar lipids	1298:1325	The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and unidentified phospholipids.
31710581	2	51	dep	Gram-stain-positive	103:121	arg1	coccus-shaped					133:145	coccus-shaped	133:145	coccus-shaped	133:145	A Gram-stain-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain N5BH11T, was isolated from a surface-sterilized sample of Mentha haplocalyx Briq.
31710581	2	51	dep	Gram-stain-positive	103:121	arg1	aerobic					124:130	aerobic	124:130	aerobic	124:130	A Gram-stain-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain N5BH11T, was isolated from a surface-sterilized sample of Mentha haplocalyx Briq.
31710581	2	51	dep	Gram-stain-positive	103:121	arg1	non-spore-forming					148:164	non-spore-forming	148:164	non-spore-forming	148:164	A Gram-stain-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain N5BH11T, was isolated from a surface-sterilized sample of Mentha haplocalyx Briq.
31710581	8	52	theme	average	826:832	arg1	%					1008:1008	74.0, 76.5 and 73.6 %	988:1008	74.0, 76.5 and 73.6 %	988:1008	The average nucleotide identity values between strain N5BH11T and the type strains of Nakamurella panacisegetis, Nakamurella multipartita and Nakamurella lactea were 74.0, 76.5 and 73.6 %, respectively.
31710581	8	52	theme	average	826:832	arg1	values					854:859	The average nucleotide identity values	822:859	The average nucleotide identity values between strain N5BH11T	822:882	The average nucleotide identity values between strain N5BH11T and the type strains of Nakamurella panacisegetis, Nakamurella multipartita and Nakamurella lactea were 74.0, 76.5 and 73.6 %, respectively.
31710581	2	53	theme	surface-sterilized	230:247	arg1	sample					249:254	a surface-sterilized sample	228:254	a surface-sterilized sample of Mentha haplocalyx Briq	228:280	A Gram-stain-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain N5BH11T, was isolated from a surface-sterilized sample of Mentha haplocalyx Briq.
31710581	8	54	theme	type	892:895	arg1	strains					897:903	the type strains	888:903	the type strains of Nakamurella panacisegetis, Nakamurella multipartita and Nakamurella lactea	888:981	The average nucleotide identity values between strain N5BH11T and the type strains of Nakamurella panacisegetis, Nakamurella multipartita and Nakamurella lactea were 74.0, 76.5 and 73.6 %, respectively.
31710581	8	54	theme	type	892:895	arg1	%					1008:1008	74.0, 76.5 and 73.6 %	988:1008	74.0, 76.5 and 73.6 %	988:1008	The average nucleotide identity values between strain N5BH11T and the type strains of Nakamurella panacisegetis, Nakamurella multipartita and Nakamurella lactea were 74.0, 76.5 and 73.6 %, respectively.
31710581	10	55	theme	meso-diaminopimelic	1226:1244	arg1	acid					1246:1249	meso-diaminopimelic acid	1226:1249	meso-diaminopimelic acid	1226:1249	The cell-wall peptidoglycan contained meso-diaminopimelic acid, and MK-8(H4) was the predominant menaquinone.
31710581	6	56	theme	Phylogenetic	557:568	arg1	analysis					570:577	Phylogenetic analysis	557:577	Phylogenetic analysis based on 16S rRNA gene sequence	557:609	Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain N5BH11T belonged to the genus Nakamurella and had the highest 16S rRNA gene sequence similarity to Nakamurella flavida DS-52T (98.1 %).
31710581	13	57	theme	phylogenetic	1561:1572	arg1	analysis					1574:1581	phylogenetic analysis	1561:1581	phylogenetic analysis	1561:1581	On the basis of the results of phylogenetic analysis and phenotypic and chemotaxonomic characteristics, strain N5BH11T represents a novel species of the genus Nakamurella, for which the name Nakamurella flava sp.
31710581	13	58	dep	results	1550:1556	arg1	the					1533:1535	the	1533:1535	the	1533:1535	On the basis of the results of phylogenetic analysis and phenotypic and chemotaxonomic characteristics, strain N5BH11T represents a novel species of the genus Nakamurella, for which the name Nakamurella flava sp.
31710581	13	58	dep	results	1550:1556	arg1	basis					1537:1541	basis	1537:1541	basis	1537:1541	On the basis of the results of phylogenetic analysis and phenotypic and chemotaxonomic characteristics, strain N5BH11T represents a novel species of the genus Nakamurella, for which the name Nakamurella flava sp.
31710581	12	59	theme	fatty	1459:1463	arg1	iso-C15 					1476:1483	iso-C15 	1476:1483	iso-C15 	1476:1483	The major fatty acids were iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and C16 : 1ω7c.
31710581	12	59	theme	fatty	1459:1463	arg1	acids					1465:1469	The major fatty acids	1449:1469	The major fatty acids	1449:1469	The major fatty acids were iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and C16 : 1ω7c.
31710581	11	60	theme	unidentified	1421:1432	arg1	phospholipids					1434:1446	unidentified phospholipids	1421:1446	unidentified phospholipids	1421:1446	The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine and unidentified phospholipids.
31710581	8	61	theme	strain	869:874	arg1	N5BH11T					876:882	strain N5BH11T	869:882	strain N5BH11T	869:882	The average nucleotide identity values between strain N5BH11T and the type strains of Nakamurella panacisegetis, Nakamurella multipartita and Nakamurella lactea were 74.0, 76.5 and 73.6 %, respectively.
31710581	6	62	theme	flavida	744:750	arg1	DS-52T					752:757	Nakamurella flavida DS-52T	732:757	Nakamurella flavida DS-52T (98.1 %)	732:766	Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain N5BH11T belonged to the genus Nakamurella and had the highest 16S rRNA gene sequence similarity to Nakamurella flavida DS-52T (98.1 %).
31710581	6	62	theme	flavida	744:750	arg1	%					765:765	98.1 %	760:765	98.1 %	760:765	Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain N5BH11T belonged to the genus Nakamurella and had the highest 16S rRNA gene sequence similarity to Nakamurella flavida DS-52T (98.1 %).
31710581	9	63	theme	multipartita	1118:1129	arg1	strains					1086:1092	the type strains	1077:1092	the type strains of N. panacisegetis, N. multipartita and N. lactea	1077:1143	The estimated DDH values between strain N5BH11T and the type strains of N. panacisegetis, N. multipartita and N. lactea were 20.3%, 21.4 and 20.2 %, respectively.
31710581	9	63	theme	multipartita	1118:1129	arg1	values					1043:1048	The estimated DDH values	1025:1048	The estimated DDH values between strain N5BH11T	1025:1071	The estimated DDH values between strain N5BH11T and the type strains of N. panacisegetis, N. multipartita and N. lactea were 20.3%, 21.4 and 20.2 %, respectively.
31710581	9	63	theme	multipartita	1118:1129	arg1	%					1154:1154	20.3%	1150:1154	20.3%	1150:1154	The estimated DDH values between strain N5BH11T and the type strains of N. panacisegetis, N. multipartita and N. lactea were 20.3%, 21.4 and 20.2 %, respectively.
31710581	2	64	theme	Briq	277:280	arg1	sample					249:254	a surface-sterilized sample	228:254	a surface-sterilized sample of Mentha haplocalyx Briq	228:280	A Gram-stain-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain N5BH11T, was isolated from a surface-sterilized sample of Mentha haplocalyx Briq.
31710581	6	65	theme	Nakamurella	732:742	arg1	DS-52T					752:757	Nakamurella flavida DS-52T	732:757	Nakamurella flavida DS-52T (98.1 %)	732:766	Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain N5BH11T belonged to the genus Nakamurella and had the highest 16S rRNA gene sequence similarity to Nakamurella flavida DS-52T (98.1 %).
31710581	6	65	theme	Nakamurella	732:742	arg1	%					765:765	98.1 %	760:765	98.1 %	760:765	Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain N5BH11T belonged to the genus Nakamurella and had the highest 16S rRNA gene sequence similarity to Nakamurella flavida DS-52T (98.1 %).
31710581	5	66	theme	Substrate	441:449	arg1	mycelia					451:457	Substrate mycelia	441:457	Substrate mycelia	441:457	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
31710581	12	67	dep	 0	1485:1486	arg1	C16 					1518:1521	C16 	1518:1521	C16 	1518:1521	The major fatty acids were iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and C16 : 1ω7c.
31710581	12	67	dep	 0	1485:1486	arg1	 0					1511:1512	 0	1511:1512	 0	1511:1512	The major fatty acids were iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and C16 : 1ω7c.
31710581	12	67	dep	 0	1485:1486	arg1	C16 					1506:1509	C16 	1506:1509	C16 	1506:1509	The major fatty acids were iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and C16 : 1ω7c.
31710581	12	67	dep	 0	1485:1486	arg1	 1ω7c					1523:1527	 1ω7c	1523:1527	 1ω7c	1523:1527	The major fatty acids were iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and C16 : 1ω7c.
31710581	9	68	theme	panacisegetis	1100:1112	arg1	strains					1086:1092	the type strains	1077:1092	the type strains of N. panacisegetis, N. multipartita and N. lactea	1077:1143	The estimated DDH values between strain N5BH11T and the type strains of N. panacisegetis, N. multipartita and N. lactea were 20.3%, 21.4 and 20.2 %, respectively.
31710581	9	68	theme	panacisegetis	1100:1112	arg1	values					1043:1048	The estimated DDH values	1025:1048	The estimated DDH values between strain N5BH11T	1025:1071	The estimated DDH values between strain N5BH11T and the type strains of N. panacisegetis, N. multipartita and N. lactea were 20.3%, 21.4 and 20.2 %, respectively.
31710581	9	68	theme	panacisegetis	1100:1112	arg1	%					1154:1154	20.3%	1150:1154	20.3%	1150:1154	The estimated DDH values between strain N5BH11T and the type strains of N. panacisegetis, N. multipartita and N. lactea were 20.3%, 21.4 and 20.2 %, respectively.
31710581	2	69	theme	haplocalyx	266:275	arg1	Briq					277:280	Mentha haplocalyx Briq	259:280	Mentha haplocalyx Briq	259:280	A Gram-stain-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain N5BH11T, was isolated from a surface-sterilized sample of Mentha haplocalyx Briq.
31710581	5	70	theme	diffusible	502:511	arg1	pigments					513:520	no diffusible pigments	499:520	no diffusible pigments	499:520	Substrate mycelia and aerial mycelia were not formed, and no diffusible pigments were observed on the media tested.
31710581	10	71	theme	predominant	1273:1283	arg1	MK-8					1256:1259	MK-8	1256:1259	MK-8(H4)	1256:1263	The cell-wall peptidoglycan contained meso-diaminopimelic acid, and MK-8(H4) was the predominant menaquinone.
31710581	10	71	theme	predominant	1273:1283	arg1	menaquinone					1285:1295	the predominant menaquinone	1269:1295	the predominant menaquinone	1269:1295	The cell-wall peptidoglycan contained meso-diaminopimelic acid, and MK-8(H4) was the predominant menaquinone.
31710581	7	72	theme	71.6 mol	811:818	arg1	%					819:819	71.6 mol%	811:819	71.6 mol%	811:819	The DNA G+C content of strain N5BH11T was 71.6 mol%.
31710581	7	72	theme	71.6 mol	811:818	arg1	content					781:787	The DNA G+C content	769:787	The DNA G+C content of strain N5BH11T	769:805	The DNA G+C content of strain N5BH11T was 71.6 mol%.
31710581	15	73	theme	49196T=CGMCC	1795:1806	arg1	N5BH11T					1780:1786	N5BH11T	1780:1786	N5BH11T (=KCTC 49196T=CGMCC 4.7524T)	1780:1815	The type strain is N5BH11T (=KCTC 49196T=CGMCC 4.7524T).
31710581	15	73	theme	49196T=CGMCC	1795:1806	arg1	4.7524T					1808:1814	=KCTC 49196T=CGMCC 4.7524T	1789:1814	=KCTC 49196T=CGMCC 4.7524T	1789:1814	The type strain is N5BH11T (=KCTC 49196T=CGMCC 4.7524T).
31710581	2	74	theme	Mentha	259:264	arg1	Briq					277:280	Mentha haplocalyx Briq	259:280	Mentha haplocalyx Briq	259:280	A Gram-stain-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain N5BH11T, was isolated from a surface-sterilized sample of Mentha haplocalyx Briq.
31710581	12	75	dep	iso-C15 	1476:1483	arg1	 0					1485:1486	 0	1485:1486	 0	1485:1486	The major fatty acids were iso-C15 : 0, anteiso-C15 : 0, C16 : 0 and C16 : 1ω7c.
31710581	3	76	theme	polyphasic	332:341	arg1	approach					343:350	a polyphasic approach	330:350	a polyphasic approach to determine its taxonomic position	330:386	collected from Guizhou, PR China and tested by a polyphasic approach to determine its taxonomic position.
31710581	10	77	contain	contained	1216:1224	arg2	acid					1246:1249	meso-diaminopimelic acid	1226:1249	meso-diaminopimelic acid	1226:1249	The cell-wall peptidoglycan contained meso-diaminopimelic acid, and MK-8(H4) was the predominant menaquinone.
31710581	10	77	contain	contained	1216:1224	arg1	peptidoglycan					1202:1214	The cell-wall peptidoglycan	1188:1214	The cell-wall peptidoglycan	1188:1214	The cell-wall peptidoglycan contained meso-diaminopimelic acid, and MK-8(H4) was the predominant menaquinone.
31710581	1	78	theme	novel	30:34	arg1	actinobacterium					47:61	a novel endophytic actinobacterium	28:61	a novel endophytic actinobacterium	28:61	nov., a novel endophytic actinobacterium isolated from Mentha haplocalyx Briq.
31710581	1	78	theme	novel	30:34	arg1	nov.					22:25	nov.	22:25	nov.	22:25	nov., a novel endophytic actinobacterium isolated from Mentha haplocalyx Briq.
31710581	6	79	theme	sequence	709:716	arg1	similarity					718:727	the highest 16S rRNA gene sequence similarity	683:727	the highest 16S rRNA gene sequence similarity to Nakamurella flavida DS-52T (98.1 %)	683:766	Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain N5BH11T belonged to the genus Nakamurella and had the highest 16S rRNA gene sequence similarity to Nakamurella flavida DS-52T (98.1 %).
31710581	6	80	theme	gene	597:600	arg1	sequence					602:609	16S rRNA gene sequence	588:609	16S rRNA gene sequence	588:609	Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain N5BH11T belonged to the genus Nakamurella and had the highest 16S rRNA gene sequence similarity to Nakamurella flavida DS-52T (98.1 %).
31710581	6	81	theme	strain	626:631	arg1	N5BH11T					633:639	strain N5BH11T	626:639	strain N5BH11T	626:639	Phylogenetic analysis based on 16S rRNA gene sequence suggested that strain N5BH11T belonged to the genus Nakamurella and had the highest 16S rRNA gene sequence similarity to Nakamurella flavida DS-52T (98.1 %).
31710581	13	82	theme	name	1716:1719	arg1	sp					1739:1740	the name Nakamurella flava sp	1712:1740	the name Nakamurella flava sp	1712:1740	On the basis of the results of phylogenetic analysis and phenotypic and chemotaxonomic characteristics, strain N5BH11T represents a novel species of the genus Nakamurella, for which the name Nakamurella flava sp.
32630764	6	0	theme	synthesis	981:989	arg1	reactions					991:999	known synthesis reactions	975:999	known synthesis reactions in the glycosylation pathway	975:1028	Based on this finding, here, we quantify the quality of a given normalization method according to how well a GGM inferred from the respective normalized data reconstructs known synthesis reactions in the glycosylation pathway.
32630764	5	1	theme	glycan	752:757	arg1	pathways					769:776	the glycan synthesis pathways	748:776	the glycan synthesis pathways in a data-driven fashion	748:801	It has been shown previously that Gaussian Graphical Models (GGMs) inferred from glycomics data are able to identify enzymatic steps in the glycan synthesis pathways in a data-driven fashion.
32630764	6	2	theme	normalization	868:880	arg1	method					882:887	a given normalization method	860:887	a given normalization method	860:887	Based on this finding, here, we quantify the quality of a given normalization method according to how well a GGM inferred from the respective normalized data reconstructs known synthesis reactions in the glycosylation pathway.
32630764	5	3	theme	Gaussian	646:653	arg1	GGMs					673:676	GGMs	673:676	GGMs	673:676	It has been shown previously that Gaussian Graphical Models (GGMs) inferred from glycomics data are able to identify enzymatic steps in the glycan synthesis pathways in a data-driven fashion.
32630764	5	3	theme	Gaussian	646:653	arg1	Models					665:670	Gaussian Graphical Models	646:670	Gaussian Graphical Models (GGMs) inferred from glycomics data	646:706	It has been shown previously that Gaussian Graphical Models (GGMs) inferred from glycomics data are able to identify enzymatic steps in the glycan synthesis pathways in a data-driven fashion.
32630764	8	4	theme	Liquid	1333:1338	arg1	Chromatography					1340:1353	Ultra High Performance Liquid Chromatography	1310:1353	Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD)	1310:1393	We analyzed 23 different normalization combinations applied to six large-scale glycomics cohorts across three experimental platforms: Liquid Chromatography - ElectroSpray Ionization - Mass Spectrometry (LC-ESI-MS), Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD), and Matrix Assisted Laser Desorption Ionization - Furier Transform Ion Cyclotron Resonance - Mass Spectrometry (MALDI-FTICR-MS).
32630764	4	5	theme	glycomics	568:576	arg1	data					578:581	glycomics data	568:581	glycomics data with an innovative approach	568:609	In this paper, we assess the quality of different normalization strategies for glycomics data with an innovative approach.
32630764	0	6	theme	Inference	98:106	arg1	Performance					75:85	Performance	75:85	Performance of Network Inference	75:106	Systematic Evaluation of Normalization Methods for Glycomics Data Based on Performance of Network Inference.
32630764	5	7	from	pathways	769:776	arg1	fashion					795:801	a data-driven fashion	781:801	a data-driven fashion	781:801	It has been shown previously that Gaussian Graphical Models (GGMs) inferred from glycomics data are able to identify enzymatic steps in the glycan synthesis pathways in a data-driven fashion.
32630764	5	8	theme	Graphical	655:663	arg1	GGMs					673:676	GGMs	673:676	GGMs	673:676	It has been shown previously that Gaussian Graphical Models (GGMs) inferred from glycomics data are able to identify enzymatic steps in the glycan synthesis pathways in a data-driven fashion.
32630764	5	8	theme	Graphical	655:663	arg1	Models					665:670	Gaussian Graphical Models	646:670	Gaussian Graphical Models (GGMs) inferred from glycomics data	646:706	It has been shown previously that Gaussian Graphical Models (GGMs) inferred from glycomics data are able to identify enzymatic steps in the glycan synthesis pathways in a data-driven fashion.
32630764	8	9	theme	Fluorescence	1360:1371	arg1	Detection					1373:1381	Fluorescence Detection	1360:1381	Fluorescence Detection (UHPLC-FLD)	1360:1393	We analyzed 23 different normalization combinations applied to six large-scale glycomics cohorts across three experimental platforms: Liquid Chromatography - ElectroSpray Ionization - Mass Spectrometry (LC-ESI-MS), Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD), and Matrix Assisted Laser Desorption Ionization - Furier Transform Ion Cyclotron Resonance - Mass Spectrometry (MALDI-FTICR-MS).
32630764	8	9	theme	Fluorescence	1360:1371	arg1	UHPLC-FLD					1384:1392	UHPLC-FLD	1384:1392	UHPLC-FLD	1384:1392	We analyzed 23 different normalization combinations applied to six large-scale glycomics cohorts across three experimental platforms: Liquid Chromatography - ElectroSpray Ionization - Mass Spectrometry (LC-ESI-MS), Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD), and Matrix Assisted Laser Desorption Ionization - Furier Transform Ion Cyclotron Resonance - Mass Spectrometry (MALDI-FTICR-MS).
32630764	9	10	theme	glycan	1572:1577	arg1	data					1579:1582	normalizing glycan data	1560:1582	normalizing glycan data using the 'Probabilistic Quotient' method followed by log-transformation, irrespective of the measurement platform	1560:1697	Based on our results, we recommend normalizing glycan data using the 'Probabilistic Quotient' method followed by log-transformation, irrespective of the measurement platform.
32630764	6	11	theme	normalized	946:955	arg1	data					957:960	the respective normalized data	931:960	the respective normalized data	931:960	Based on this finding, here, we quantify the quality of a given normalization method according to how well a GGM inferred from the respective normalized data reconstructs known synthesis reactions in the glycosylation pathway.
32630764	0	12	theme	Network	90:96	arg1	Inference					98:106	Network Inference	90:106	Network Inference	90:106	Systematic Evaluation of Normalization Methods for Glycomics Data Based on Performance of Network Inference.
32630764	9	13	theme	Probabilistic	1595:1607	arg1	method					1619:1624	the 'Probabilistic Quotient' method	1590:1624	the 'Probabilistic Quotient' method followed by log-transformation, irrespective of the measurement platform	1590:1697	Based on our results, we recommend normalizing glycan data using the 'Probabilistic Quotient' method followed by log-transformation, irrespective of the measurement platform.
32630764	6	14	from	reactions	991:999	arg1	pathway					1022:1028	the glycosylation pathway	1004:1028	the glycosylation pathway	1004:1028	Based on this finding, here, we quantify the quality of a given normalization method according to how well a GGM inferred from the respective normalized data reconstructs known synthesis reactions in the glycosylation pathway.
32630764	2	15	theme	signal	300:305	arg1	normalization					339:351	normalization	339:351	normalization	339:351	The removal of this non-biological signal from the data is referred to as normalization.
32630764	2	15	theme	signal	300:305	arg1	removal					269:275	The removal	265:275	The removal of this non-biological signal from the data	265:319	The removal of this non-biological signal from the data is referred to as normalization.
32630764	6	16	theme	respective	935:944	arg1	data					957:960	the respective normalized data	931:960	the respective normalized data	931:960	Based on this finding, here, we quantify the quality of a given normalization method according to how well a GGM inferred from the respective normalized data reconstructs known synthesis reactions in the glycosylation pathway.
32630764	8	17	theme	large-scale	1162:1172	arg1	cohorts					1184:1190	six large-scale glycomics cohorts	1158:1190	six large-scale glycomics cohorts	1158:1190	We analyzed 23 different normalization combinations applied to six large-scale glycomics cohorts across three experimental platforms: Liquid Chromatography - ElectroSpray Ionization - Mass Spectrometry (LC-ESI-MS), Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD), and Matrix Assisted Laser Desorption Ionization - Furier Transform Ion Cyclotron Resonance - Mass Spectrometry (MALDI-FTICR-MS).
32630764	2	18	theme	non-biological	285:298	arg1	signal					300:305	this non-biological signal	280:305	this non-biological signal from the data	280:319	The removal of this non-biological signal from the data is referred to as normalization.
32630764	3	19	theme	glycomics	443:451	arg1	data					453:456	glycomics data	443:456	glycomics data	443:456	Contrary to other omics data types, a systematic evaluation of normalization options for glycomics data has not been published so far.
32630764	5	20	theme	enzymatic	729:737	arg1	steps					739:743	enzymatic steps	729:743	enzymatic steps in the glycan synthesis pathways in a data-driven fashion	729:801	It has been shown previously that Gaussian Graphical Models (GGMs) inferred from glycomics data are able to identify enzymatic steps in the glycan synthesis pathways in a data-driven fashion.
32630764	8	21	theme	glycomics	1174:1182	arg1	cohorts					1184:1190	six large-scale glycomics cohorts	1158:1190	six large-scale glycomics cohorts	1158:1190	We analyzed 23 different normalization combinations applied to six large-scale glycomics cohorts across three experimental platforms: Liquid Chromatography - ElectroSpray Ionization - Mass Spectrometry (LC-ESI-MS), Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD), and Matrix Assisted Laser Desorption Ionization - Furier Transform Ion Cyclotron Resonance - Mass Spectrometry (MALDI-FTICR-MS).
32630764	5	22	theme	data-driven	783:793	arg1	fashion					795:801	a data-driven fashion	781:801	a data-driven fashion	781:801	It has been shown previously that Gaussian Graphical Models (GGMs) inferred from glycomics data are able to identify enzymatic steps in the glycan synthesis pathways in a data-driven fashion.
32630764	8	23	theme	normalization	1120:1132	arg1	combinations					1134:1145	23 different normalization combinations	1107:1145	23 different normalization combinations applied to six large-scale glycomics cohorts across three experimental platforms	1107:1226	We analyzed 23 different normalization combinations applied to six large-scale glycomics cohorts across three experimental platforms: Liquid Chromatography - ElectroSpray Ionization - Mass Spectrometry (LC-ESI-MS), Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD), and Matrix Assisted Laser Desorption Ionization - Furier Transform Ion Cyclotron Resonance - Mass Spectrometry (MALDI-FTICR-MS).
32630764	8	24	theme	ElectroSpray	1253:1264	arg1	LC-ESI-MS					1298:1306	LC-ESI-MS	1298:1306	LC-ESI-MS	1298:1306	We analyzed 23 different normalization combinations applied to six large-scale glycomics cohorts across three experimental platforms: Liquid Chromatography - ElectroSpray Ionization - Mass Spectrometry (LC-ESI-MS), Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD), and Matrix Assisted Laser Desorption Ionization - Furier Transform Ion Cyclotron Resonance - Mass Spectrometry (MALDI-FTICR-MS).
32630764	8	24	theme	ElectroSpray	1253:1264	arg1	Ionization					1266:1275	ElectroSpray Ionization	1253:1275	ElectroSpray Ionization	1253:1275	We analyzed 23 different normalization combinations applied to six large-scale glycomics cohorts across three experimental platforms: Liquid Chromatography - ElectroSpray Ionization - Mass Spectrometry (LC-ESI-MS), Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD), and Matrix Assisted Laser Desorption Ionization - Furier Transform Ion Cyclotron Resonance - Mass Spectrometry (MALDI-FTICR-MS).
32630764	3	25	theme	options	431:437	arg1	evaluation					403:412	a systematic evaluation	390:412	a systematic evaluation of normalization options for glycomics data	390:456	Contrary to other omics data types, a systematic evaluation of normalization options for glycomics data has not been published so far.
32630764	0	26	theme	Systematic	0:9	arg1	Evaluation					11:20	Systematic Evaluation	0:20	Systematic Evaluation of Normalization Methods for Glycomics Data	0:64	Systematic Evaluation of Normalization Methods for Glycomics Data Based on Performance of Network Inference.
32630764	4	27	theme	normalization	539:551	arg1	strategies					553:562	different normalization strategies	529:562	different normalization strategies for glycomics data with an innovative approach	529:609	In this paper, we assess the quality of different normalization strategies for glycomics data with an innovative approach.
32630764	7	28	theme	biological	1063:1072	arg1	measure					1074:1080	a biological measure	1061:1080	a biological measure of goodness	1061:1092	The method therefore exploits a biological measure of goodness.
32630764	1	29	theme	technical	193:201	arg1	variation					203:211	technical variation	193:211	technical variation due to fluctuations in the experimental conditions	193:262	Glycomics measurements, like all other high-throughput technologies, are subject to technical variation due to fluctuations in the experimental conditions.
32630764	3	30	theme	data	378:381	arg1	types					383:387	other omics data types	366:387	other omics data types	366:387	Contrary to other omics data types, a systematic evaluation of normalization options for glycomics data has not been published so far.
32630764	4	31	theme	different	529:537	arg1	strategies					553:562	different normalization strategies	529:562	different normalization strategies for glycomics data with an innovative approach	529:609	In this paper, we assess the quality of different normalization strategies for glycomics data with an innovative approach.
32630764	9	32	theme	Quotient	1609:1616	arg1	method					1619:1624	the 'Probabilistic Quotient' method	1590:1624	the 'Probabilistic Quotient' method followed by log-transformation, irrespective of the measurement platform	1590:1697	Based on our results, we recommend normalizing glycan data using the 'Probabilistic Quotient' method followed by log-transformation, irrespective of the measurement platform.
32630764	6	33	theme	given	862:866	arg1	method					882:887	a given normalization method	860:887	a given normalization method	860:887	Based on this finding, here, we quantify the quality of a given normalization method according to how well a GGM inferred from the respective normalized data reconstructs known synthesis reactions in the glycosylation pathway.
32630764	9	34	theme	measurement	1678:1688	arg1	platform					1690:1697	the measurement platform	1674:1697	the measurement platform	1674:1697	Based on our results, we recommend normalizing glycan data using the 'Probabilistic Quotient' method followed by log-transformation, irrespective of the measurement platform.
32630764	5	35	theme	glycomics	693:701	arg1	data					703:706	glycomics data	693:706	glycomics data	693:706	It has been shown previously that Gaussian Graphical Models (GGMs) inferred from glycomics data are able to identify enzymatic steps in the glycan synthesis pathways in a data-driven fashion.
32630764	8	36	theme	Cyclotron	1467:1475	arg1	Resonance					1477:1485	Ion Cyclotron Resonance	1463:1485	Ion Cyclotron Resonance	1463:1485	We analyzed 23 different normalization combinations applied to six large-scale glycomics cohorts across three experimental platforms: Liquid Chromatography - ElectroSpray Ionization - Mass Spectrometry (LC-ESI-MS), Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD), and Matrix Assisted Laser Desorption Ionization - Furier Transform Ion Cyclotron Resonance - Mass Spectrometry (MALDI-FTICR-MS).
32630764	1	37	theme	Glycomics	109:117	arg1	measurements					119:130	Glycomics measurements	109:130	Glycomics measurements	109:130	Glycomics measurements, like all other high-throughput technologies, are subject to technical variation due to fluctuations in the experimental conditions.
32630764	6	38	theme	method	882:887	arg1	quality					849:855	the quality	845:855	the quality of a given normalization method	845:887	Based on this finding, here, we quantify the quality of a given normalization method according to how well a GGM inferred from the respective normalized data reconstructs known synthesis reactions in the glycosylation pathway.
32630764	1	39	theme	due	213:215	arg1	variation					203:211	technical variation	193:211	technical variation due to fluctuations in the experimental conditions	193:262	Glycomics measurements, like all other high-throughput technologies, are subject to technical variation due to fluctuations in the experimental conditions.
32630764	5	40	theme	synthesis	759:767	arg1	pathways					769:776	the glycan synthesis pathways	748:776	the glycan synthesis pathways in a data-driven fashion	748:801	It has been shown previously that Gaussian Graphical Models (GGMs) inferred from glycomics data are able to identify enzymatic steps in the glycan synthesis pathways in a data-driven fashion.
32630764	4	41	theme	strategies	553:562	arg1	quality					518:524	the quality	514:524	the quality of different normalization strategies for glycomics data with an innovative approach	514:609	In this paper, we assess the quality of different normalization strategies for glycomics data with an innovative approach.
32630764	8	42	theme	Ion	1463:1465	arg1	Resonance					1477:1485	Ion Cyclotron Resonance	1463:1485	Ion Cyclotron Resonance	1463:1485	We analyzed 23 different normalization combinations applied to six large-scale glycomics cohorts across three experimental platforms: Liquid Chromatography - ElectroSpray Ionization - Mass Spectrometry (LC-ESI-MS), Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD), and Matrix Assisted Laser Desorption Ionization - Furier Transform Ion Cyclotron Resonance - Mass Spectrometry (MALDI-FTICR-MS).
32630764	8	43	theme	Assisted	1407:1414	arg1	Ionization					1433:1442	Matrix Assisted Laser Desorption Ionization	1400:1442	Matrix Assisted Laser Desorption Ionization	1400:1442	We analyzed 23 different normalization combinations applied to six large-scale glycomics cohorts across three experimental platforms: Liquid Chromatography - ElectroSpray Ionization - Mass Spectrometry (LC-ESI-MS), Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD), and Matrix Assisted Laser Desorption Ionization - Furier Transform Ion Cyclotron Resonance - Mass Spectrometry (MALDI-FTICR-MS).
32630764	2	44	from	removal	269:275	arg1	data					316:319	the data	312:319	the data	312:319	The removal of this non-biological signal from the data is referred to as normalization.
32630764	6	45	theme	glycosylation	1008:1020	arg1	pathway					1022:1028	the glycosylation pathway	1004:1028	the glycosylation pathway	1004:1028	Based on this finding, here, we quantify the quality of a given normalization method according to how well a GGM inferred from the respective normalized data reconstructs known synthesis reactions in the glycosylation pathway.
32630764	0	46	theme	Methods	39:45	arg1	Evaluation					11:20	Systematic Evaluation	0:20	Systematic Evaluation of Normalization Methods for Glycomics Data	0:64	Systematic Evaluation of Normalization Methods for Glycomics Data Based on Performance of Network Inference.
32630764	3	47	theme	normalization	417:429	arg1	options					431:437	normalization options	417:437	normalization options for glycomics data	417:456	Contrary to other omics data types, a systematic evaluation of normalization options for glycomics data has not been published so far.
32630764	0	48	theme	Normalization	25:37	arg1	Methods					39:45	Normalization Methods	25:45	Normalization Methods	25:45	Systematic Evaluation of Normalization Methods for Glycomics Data Based on Performance of Network Inference.
32630764	3	49	theme	systematic	392:401	arg1	evaluation					403:412	a systematic evaluation	390:412	a systematic evaluation of normalization options for glycomics data	390:456	Contrary to other omics data types, a systematic evaluation of normalization options for glycomics data has not been published so far.
32630764	3	50	theme	other	366:370	arg1	types					383:387	other omics data types	366:387	other omics data types	366:387	Contrary to other omics data types, a systematic evaluation of normalization options for glycomics data has not been published so far.
32630764	8	51	theme	experimental	1205:1216	arg1	platforms					1218:1226	three experimental platforms	1199:1226	three experimental platforms	1199:1226	We analyzed 23 different normalization combinations applied to six large-scale glycomics cohorts across three experimental platforms: Liquid Chromatography - ElectroSpray Ionization - Mass Spectrometry (LC-ESI-MS), Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD), and Matrix Assisted Laser Desorption Ionization - Furier Transform Ion Cyclotron Resonance - Mass Spectrometry (MALDI-FTICR-MS).
32630764	10	52	with	associations	1833:1844	arg1	age					1851:1853	age	1851:1853	age	1851:1853	This recommendation is further supported by an additional analysis, where we ranked normalization methods based on their statistical associations with age, a factor known to associate with glycomics measurements.
32630764	10	52	with	associations	1833:1844	arg1	factor					1858:1863	a factor	1856:1863	a factor known to associate with glycomics measurements	1856:1910	This recommendation is further supported by an additional analysis, where we ranked normalization methods based on their statistical associations with age, a factor known to associate with glycomics measurements.
32630764	8	53	theme	Desorption	1422:1431	arg1	Ionization					1433:1442	Matrix Assisted Laser Desorption Ionization	1400:1442	Matrix Assisted Laser Desorption Ionization	1400:1442	We analyzed 23 different normalization combinations applied to six large-scale glycomics cohorts across three experimental platforms: Liquid Chromatography - ElectroSpray Ionization - Mass Spectrometry (LC-ESI-MS), Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD), and Matrix Assisted Laser Desorption Ionization - Furier Transform Ion Cyclotron Resonance - Mass Spectrometry (MALDI-FTICR-MS).
32630764	1	54	from	fluctuations	220:231	arg1	conditions					253:262	the experimental conditions	236:262	the experimental conditions	236:262	Glycomics measurements, like all other high-throughput technologies, are subject to technical variation due to fluctuations in the experimental conditions.
32630764	10	55	theme	glycomics	1889:1897	arg1	measurements					1899:1910	glycomics measurements	1889:1910	glycomics measurements	1889:1910	This recommendation is further supported by an additional analysis, where we ranked normalization methods based on their statistical associations with age, a factor known to associate with glycomics measurements.
32630764	0	56	theme	Glycomics	51:59	arg1	Data					61:64	Glycomics Data	51:64	Glycomics Data	51:64	Systematic Evaluation of Normalization Methods for Glycomics Data Based on Performance of Network Inference.
32630764	8	57	with	Chromatography	1340:1353	arg1	Detection					1373:1381	Fluorescence Detection	1360:1381	Fluorescence Detection (UHPLC-FLD)	1360:1393	We analyzed 23 different normalization combinations applied to six large-scale glycomics cohorts across three experimental platforms: Liquid Chromatography - ElectroSpray Ionization - Mass Spectrometry (LC-ESI-MS), Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD), and Matrix Assisted Laser Desorption Ionization - Furier Transform Ion Cyclotron Resonance - Mass Spectrometry (MALDI-FTICR-MS).
32630764	8	57	with	Chromatography	1340:1353	arg1	UHPLC-FLD					1384:1392	UHPLC-FLD	1384:1392	UHPLC-FLD	1384:1392	We analyzed 23 different normalization combinations applied to six large-scale glycomics cohorts across three experimental platforms: Liquid Chromatography - ElectroSpray Ionization - Mass Spectrometry (LC-ESI-MS), Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD), and Matrix Assisted Laser Desorption Ionization - Furier Transform Ion Cyclotron Resonance - Mass Spectrometry (MALDI-FTICR-MS).
32630764	8	58	theme	Laser	1416:1420	arg1	Ionization					1433:1442	Matrix Assisted Laser Desorption Ionization	1400:1442	Matrix Assisted Laser Desorption Ionization	1400:1442	We analyzed 23 different normalization combinations applied to six large-scale glycomics cohorts across three experimental platforms: Liquid Chromatography - ElectroSpray Ionization - Mass Spectrometry (LC-ESI-MS), Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD), and Matrix Assisted Laser Desorption Ionization - Furier Transform Ion Cyclotron Resonance - Mass Spectrometry (MALDI-FTICR-MS).
32630764	9	59	theme	platform	1690:1697	arg1	irrespective					1658:1669	irrespective	1658:1669	irrespective	1658:1669	Based on our results, we recommend normalizing glycan data using the 'Probabilistic Quotient' method followed by log-transformation, irrespective of the measurement platform.
32630764	7	60	theme	goodness	1085:1092	arg1	measure					1074:1080	a biological measure	1061:1080	a biological measure of goodness	1061:1092	The method therefore exploits a biological measure of goodness.
32630764	9	61	theme	normalizing	1560:1570	arg1	data					1579:1582	normalizing glycan data	1560:1582	normalizing glycan data using the 'Probabilistic Quotient' method followed by log-transformation, irrespective of the measurement platform	1560:1697	Based on our results, we recommend normalizing glycan data using the 'Probabilistic Quotient' method followed by log-transformation, irrespective of the measurement platform.
32630764	9	62	theme	irrespective	1658:1669	arg1	log-transformation					1638:1655	log-transformation	1638:1655	log-transformation	1638:1655	Based on our results, we recommend normalizing glycan data using the 'Probabilistic Quotient' method followed by log-transformation, irrespective of the measurement platform.
32630764	8	63	theme	Mass	1489:1492	arg1	Spectrometry					1494:1505	Mass Spectrometry	1489:1505	Mass Spectrometry (MALDI-FTICR-MS)	1489:1522	We analyzed 23 different normalization combinations applied to six large-scale glycomics cohorts across three experimental platforms: Liquid Chromatography - ElectroSpray Ionization - Mass Spectrometry (LC-ESI-MS), Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD), and Matrix Assisted Laser Desorption Ionization - Furier Transform Ion Cyclotron Resonance - Mass Spectrometry (MALDI-FTICR-MS).
32630764	8	63	theme	Mass	1489:1492	arg1	MALDI-FTICR-MS					1508:1521	MALDI-FTICR-MS	1508:1521	MALDI-FTICR-MS	1508:1521	We analyzed 23 different normalization combinations applied to six large-scale glycomics cohorts across three experimental platforms: Liquid Chromatography - ElectroSpray Ionization - Mass Spectrometry (LC-ESI-MS), Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD), and Matrix Assisted Laser Desorption Ionization - Furier Transform Ion Cyclotron Resonance - Mass Spectrometry (MALDI-FTICR-MS).
32630764	10	64	theme	normalization	1784:1796	arg1	methods					1798:1804	normalization methods	1784:1804	normalization methods based on their statistical associations with age, a factor known to associate with glycomics measurements	1784:1910	This recommendation is further supported by an additional analysis, where we ranked normalization methods based on their statistical associations with age, a factor known to associate with glycomics measurements.
32630764	8	65	theme	Matrix	1400:1405	arg1	Ionization					1433:1442	Matrix Assisted Laser Desorption Ionization	1400:1442	Matrix Assisted Laser Desorption Ionization	1400:1442	We analyzed 23 different normalization combinations applied to six large-scale glycomics cohorts across three experimental platforms: Liquid Chromatography - ElectroSpray Ionization - Mass Spectrometry (LC-ESI-MS), Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD), and Matrix Assisted Laser Desorption Ionization - Furier Transform Ion Cyclotron Resonance - Mass Spectrometry (MALDI-FTICR-MS).
32630764	8	66	theme	Ultra	1310:1314	arg1	Chromatography					1340:1353	Ultra High Performance Liquid Chromatography	1310:1353	Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD)	1310:1393	We analyzed 23 different normalization combinations applied to six large-scale glycomics cohorts across three experimental platforms: Liquid Chromatography - ElectroSpray Ionization - Mass Spectrometry (LC-ESI-MS), Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD), and Matrix Assisted Laser Desorption Ionization - Furier Transform Ion Cyclotron Resonance - Mass Spectrometry (MALDI-FTICR-MS).
32630764	5	67	from	steps	739:743	arg1	pathways					769:776	the glycan synthesis pathways	748:776	the glycan synthesis pathways in a data-driven fashion	748:801	It has been shown previously that Gaussian Graphical Models (GGMs) inferred from glycomics data are able to identify enzymatic steps in the glycan synthesis pathways in a data-driven fashion.
32630764	8	68	theme	Performance	1321:1331	arg1	Chromatography					1340:1353	Ultra High Performance Liquid Chromatography	1310:1353	Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD)	1310:1393	We analyzed 23 different normalization combinations applied to six large-scale glycomics cohorts across three experimental platforms: Liquid Chromatography - ElectroSpray Ionization - Mass Spectrometry (LC-ESI-MS), Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD), and Matrix Assisted Laser Desorption Ionization - Furier Transform Ion Cyclotron Resonance - Mass Spectrometry (MALDI-FTICR-MS).
32630764	8	69	theme	High	1316:1319	arg1	Chromatography					1340:1353	Ultra High Performance Liquid Chromatography	1310:1353	Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD)	1310:1393	We analyzed 23 different normalization combinations applied to six large-scale glycomics cohorts across three experimental platforms: Liquid Chromatography - ElectroSpray Ionization - Mass Spectrometry (LC-ESI-MS), Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD), and Matrix Assisted Laser Desorption Ionization - Furier Transform Ion Cyclotron Resonance - Mass Spectrometry (MALDI-FTICR-MS).
32630764	10	70	theme	statistical	1821:1831	arg1	associations					1833:1844	their statistical associations	1815:1844	their statistical associations with age, a factor known to associate with glycomics measurements	1815:1910	This recommendation is further supported by an additional analysis, where we ranked normalization methods based on their statistical associations with age, a factor known to associate with glycomics measurements.
32630764	4	71	theme	innovative	591:600	arg1	approach					602:609	an innovative approach	588:609	an innovative approach	588:609	In this paper, we assess the quality of different normalization strategies for glycomics data with an innovative approach.
32630764	8	72	theme	different	1110:1118	arg1	combinations					1134:1145	23 different normalization combinations	1107:1145	23 different normalization combinations applied to six large-scale glycomics cohorts across three experimental platforms	1107:1226	We analyzed 23 different normalization combinations applied to six large-scale glycomics cohorts across three experimental platforms: Liquid Chromatography - ElectroSpray Ionization - Mass Spectrometry (LC-ESI-MS), Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD), and Matrix Assisted Laser Desorption Ionization - Furier Transform Ion Cyclotron Resonance - Mass Spectrometry (MALDI-FTICR-MS).
32630764	4	73	with	data	578:581	arg1	approach					602:609	an innovative approach	588:609	an innovative approach	588:609	In this paper, we assess the quality of different normalization strategies for glycomics data with an innovative approach.
32630764	8	74	theme	Mass	1279:1282	arg1	Spectrometry					1284:1295	Mass Spectrometry	1279:1295	Mass Spectrometry	1279:1295	We analyzed 23 different normalization combinations applied to six large-scale glycomics cohorts across three experimental platforms: Liquid Chromatography - ElectroSpray Ionization - Mass Spectrometry (LC-ESI-MS), Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD), and Matrix Assisted Laser Desorption Ionization - Furier Transform Ion Cyclotron Resonance - Mass Spectrometry (MALDI-FTICR-MS).
32630764	1	75	theme	other	142:146	arg1	technologies					164:175	all other high-throughput technologies	138:175	all other high-throughput technologies	138:175	Glycomics measurements, like all other high-throughput technologies, are subject to technical variation due to fluctuations in the experimental conditions.
32630764	1	76	theme	experimental	240:251	arg1	conditions					253:262	the experimental conditions	236:262	the experimental conditions	236:262	Glycomics measurements, like all other high-throughput technologies, are subject to technical variation due to fluctuations in the experimental conditions.
32630764	8	77	theme	Liquid	1229:1234	arg1	Chromatography					1236:1249	Liquid Chromatography	1229:1249	Liquid Chromatography	1229:1249	We analyzed 23 different normalization combinations applied to six large-scale glycomics cohorts across three experimental platforms: Liquid Chromatography - ElectroSpray Ionization - Mass Spectrometry (LC-ESI-MS), Ultra High Performance Liquid Chromatography with Fluorescence Detection (UHPLC-FLD), and Matrix Assisted Laser Desorption Ionization - Furier Transform Ion Cyclotron Resonance - Mass Spectrometry (MALDI-FTICR-MS).
32630764	10	78	theme	additional	1747:1756	arg1	analysis					1758:1765	an additional analysis	1744:1765	an additional analysis	1744:1765	This recommendation is further supported by an additional analysis, where we ranked normalization methods based on their statistical associations with age, a factor known to associate with glycomics measurements.
32630764	6	79	theme	known	975:979	arg1	reactions					991:999	known synthesis reactions	975:999	known synthesis reactions in the glycosylation pathway	975:1028	Based on this finding, here, we quantify the quality of a given normalization method according to how well a GGM inferred from the respective normalized data reconstructs known synthesis reactions in the glycosylation pathway.
32630764	1	80	theme	high-throughput	148:162	arg1	technologies					164:175	all other high-throughput technologies	138:175	all other high-throughput technologies	138:175	Glycomics measurements, like all other high-throughput technologies, are subject to technical variation due to fluctuations in the experimental conditions.
32630764	2	81	from	data	316:319	arg1	signal					300:305	this non-biological signal	280:305	this non-biological signal from the data	280:319	The removal of this non-biological signal from the data is referred to as normalization.
32630764	2	81	from	data	316:319	arg1	normalization					339:351	normalization	339:351	normalization	339:351	The removal of this non-biological signal from the data is referred to as normalization.
32630764	2	81	from	data	316:319	arg1	removal					269:275	The removal	265:275	The removal of this non-biological signal from the data	265:319	The removal of this non-biological signal from the data is referred to as normalization.
32630764	3	82	theme	omics	372:376	arg1	types					383:387	other omics data types	366:387	other omics data types	366:387	Contrary to other omics data types, a systematic evaluation of normalization options for glycomics data has not been published so far.
34542110	7	0	theme	higher	805:810	arg1	loss					824:827	higher body weight loss	805:827	higher body weight loss	805:827	Ketogenic diet substantially worsened colitis, in terms of higher body weight loss, DAI scores and histological scores as well as colon length shortening.
34542110	11	1	theme	glycocholic	1654:1664	arg1	metabolites					1550:1560	metabolites	1550:1560	metabolites in the bile secretion such as ouabain	1550:1598	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	11	1	theme	glycocholic	1654:1664	arg1	ouabain					1592:1598	ouabain	1592:1598	ouabain	1592:1598	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	11	1	theme	glycocholic	1654:1664	arg1	acid					1666:1669	glycocholic acid	1654:1669	glycocholic acid	1654:1669	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	10	2	theme	pathogenic	1326:1335	arg1	taxa					1337:1340	pathogenic taxa	1326:1340	pathogenic taxa such as Proteobacteria, Enterobacteriaceae, Helicobacter and Escherichia-Shigella	1326:1422	Pretreatment with ketogenic diet alters the bacterial abundance, increasing pathogenic taxa such as Proteobacteria, Enterobacteriaceae, Helicobacter and Escherichia-Shigella and decreasing potential beneficial taxa such as Erysipelotrichaceae.
34542110	10	2	theme	pathogenic	1326:1335	arg1	Proteobacteria					1350:1363	Proteobacteria	1350:1363	Proteobacteria	1350:1363	Pretreatment with ketogenic diet alters the bacterial abundance, increasing pathogenic taxa such as Proteobacteria, Enterobacteriaceae, Helicobacter and Escherichia-Shigella and decreasing potential beneficial taxa such as Erysipelotrichaceae.
34542110	10	2	theme	pathogenic	1326:1335	arg1	Escherichia-Shigella					1403:1422	Escherichia-Shigella	1403:1422	Escherichia-Shigella	1403:1422	Pretreatment with ketogenic diet alters the bacterial abundance, increasing pathogenic taxa such as Proteobacteria, Enterobacteriaceae, Helicobacter and Escherichia-Shigella and decreasing potential beneficial taxa such as Erysipelotrichaceae.
34542110	10	2	theme	pathogenic	1326:1335	arg1	Helicobacter					1386:1397	Helicobacter	1386:1397	Helicobacter	1386:1397	Pretreatment with ketogenic diet alters the bacterial abundance, increasing pathogenic taxa such as Proteobacteria, Enterobacteriaceae, Helicobacter and Escherichia-Shigella and decreasing potential beneficial taxa such as Erysipelotrichaceae.
34542110	10	2	theme	pathogenic	1326:1335	arg1	Enterobacteriaceae					1366:1383	Enterobacteriaceae	1366:1383	Enterobacteriaceae	1366:1383	Pretreatment with ketogenic diet alters the bacterial abundance, increasing pathogenic taxa such as Proteobacteria, Enterobacteriaceae, Helicobacter and Escherichia-Shigella and decreasing potential beneficial taxa such as Erysipelotrichaceae.
34542110	9	3	theme	intestinal	1098:1107	arg1	permeability					1109:1120	Increased intestinal permeability	1088:1120	Increased intestinal permeability	1088:1120	Increased intestinal permeability and decreased expressions of intestinal epithelial barrier associated genes were observed due to ketogenic diet administration.
34542110	1	4	theme	inflammatory	174:185	arg1	disease					143:149	Inflammatory bowel disease	124:149	Inflammatory bowel disease (IBD)	124:155	Inflammatory bowel disease (IBD) is an idiopathic inflammatory disease with a high incidence.
34542110	1	4	theme	inflammatory	174:185	arg1	disease					187:193	an idiopathic inflammatory disease	160:193	an idiopathic inflammatory disease with a high incidence	160:215	Inflammatory bowel disease (IBD) is an idiopathic inflammatory disease with a high incidence.
34542110	12	5	theme	intestinal	1985:1994	arg1	barrier					1996:2002	the intestinal barrier	1981:2002	the intestinal barrier	1981:2002	These results suggest that ketogenic diet aggravates DSS-induced colitis in mice by increasing intestinal and systemic inflammation, and disrupting the intestinal barrier, which results from modulated gut microbiota and metabolism.
34542110	8	6	theme	inflammatory	927:938	arg1	IL-10					1004:1008	IL-10	1004:1008	IL-10	1004:1008	Levels of serum and colon inflammatory cytokines and chemokines (IL-1α, IL-6, TNF-α, IL-17, GM-CSF and IL-10) were significantly up-regulated in mice treated with ketogenic diet and DSS.
34542110	8	6	theme	inflammatory	927:938	arg1	IL-1α					966:970	IL-1α	966:970	IL-1α	966:970	Levels of serum and colon inflammatory cytokines and chemokines (IL-1α, IL-6, TNF-α, IL-17, GM-CSF and IL-10) were significantly up-regulated in mice treated with ketogenic diet and DSS.
34542110	8	6	theme	inflammatory	927:938	arg1	IL-17					986:990	IL-17	986:990	IL-17	986:990	Levels of serum and colon inflammatory cytokines and chemokines (IL-1α, IL-6, TNF-α, IL-17, GM-CSF and IL-10) were significantly up-regulated in mice treated with ketogenic diet and DSS.
34542110	8	6	theme	inflammatory	927:938	arg1	GM-CSF					993:998	GM-CSF	993:998	GM-CSF	993:998	Levels of serum and colon inflammatory cytokines and chemokines (IL-1α, IL-6, TNF-α, IL-17, GM-CSF and IL-10) were significantly up-regulated in mice treated with ketogenic diet and DSS.
34542110	8	6	theme	inflammatory	927:938	arg1	cytokines					940:948	colon inflammatory cytokines	921:948	colon inflammatory cytokines	921:948	Levels of serum and colon inflammatory cytokines and chemokines (IL-1α, IL-6, TNF-α, IL-17, GM-CSF and IL-10) were significantly up-regulated in mice treated with ketogenic diet and DSS.
34542110	8	6	theme	inflammatory	927:938	arg1	IL-6					973:976	IL-6	973:976	IL-6	973:976	Levels of serum and colon inflammatory cytokines and chemokines (IL-1α, IL-6, TNF-α, IL-17, GM-CSF and IL-10) were significantly up-regulated in mice treated with ketogenic diet and DSS.
34542110	8	6	theme	inflammatory	927:938	arg1	TNF-α					979:983	TNF-α	979:983	TNF-α	979:983	Levels of serum and colon inflammatory cytokines and chemokines (IL-1α, IL-6, TNF-α, IL-17, GM-CSF and IL-10) were significantly up-regulated in mice treated with ketogenic diet and DSS.
34542110	11	7	theme	arachidic	1783:1791	arg1	acid					1793:1796	arachidic acid	1783:1796	arachidic acid	1783:1796	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	5	8	theme	last	636:639	arg1	week					641:644	the last week	632:644	the last week	632:644	C57BL/6 mice were given a ketogenic diet or a control diet for a month and IBD was induced by 2% DSS in drinking water in the last week.
34542110	10	9	dep	alters	1283:1288	arg1	decreasing					1428:1437	decreasing	1428:1437	decreasing potential beneficial taxa such as Erysipelotrichaceae	1428:1491	Pretreatment with ketogenic diet alters the bacterial abundance, increasing pathogenic taxa such as Proteobacteria, Enterobacteriaceae, Helicobacter and Escherichia-Shigella and decreasing potential beneficial taxa such as Erysipelotrichaceae.
34542110	10	9	dep	alters	1283:1288	arg1	increasing					1315:1324	increasing	1315:1324	increasing pathogenic taxa such as Proteobacteria, Enterobacteriaceae, Helicobacter and Escherichia-Shigella	1315:1422	Pretreatment with ketogenic diet alters the bacterial abundance, increasing pathogenic taxa such as Proteobacteria, Enterobacteriaceae, Helicobacter and Escherichia-Shigella and decreasing potential beneficial taxa such as Erysipelotrichaceae.
34542110	12	10	theme	systemic	1943:1950	arg1	inflammation					1952:1963	intestinal and systemic inflammation	1928:1963	inflammation	1952:1963	These results suggest that ketogenic diet aggravates DSS-induced colitis in mice by increasing intestinal and systemic inflammation, and disrupting the intestinal barrier, which results from modulated gut microbiota and metabolism.
34542110	9	11	theme	intestinal	1151:1160	arg1	barrier					1173:1179	intestinal epithelial barrier	1151:1179	intestinal epithelial barrier associated genes	1151:1196	Increased intestinal permeability and decreased expressions of intestinal epithelial barrier associated genes were observed due to ketogenic diet administration.
34542110	2	12	theme	Multiple	218:225	arg1	factors					227:233	Multiple factors	218:233	Multiple factors including dietary composition	218:263	Multiple factors including dietary composition contribute to its occurrence.
34542110	2	12	theme	Multiple	218:225	arg1	composition					253:263	dietary composition	245:263	dietary composition	245:263	Multiple factors including dietary composition contribute to its occurrence.
34542110	1	13	theme	high	202:205	arg1	incidence					207:215	a high incidence	200:215	a high incidence	200:215	Inflammatory bowel disease (IBD) is an idiopathic inflammatory disease with a high incidence.
34542110	3	14	theme	ketogenic	305:313	arg1	diet					315:318	ketogenic diet	305:318	ketogenic diet which consists of a high proportion of fat and low carbohydrates	305:383	Recently, ketogenic diet which consists of a high proportion of fat and low carbohydrates has gained great popularity.
34542110	9	15	theme	barrier	1173:1179	arg1	genes					1192:1196	intestinal epithelial barrier associated genes	1151:1196	intestinal epithelial barrier associated genes	1151:1196	Increased intestinal permeability and decreased expressions of intestinal epithelial barrier associated genes were observed due to ketogenic diet administration.
34542110	11	16	from	metabolites	1550:1560	arg1	secretion					1574:1582	the bile secretion	1565:1582	the bile secretion	1565:1582	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	5	17	theme	control	556:562	arg1	diet					564:567	a control diet	554:567	a control diet	554:567	C57BL/6 mice were given a ketogenic diet or a control diet for a month and IBD was induced by 2% DSS in drinking water in the last week.
34542110	11	18	theme	fatty	1747:1751	arg1	acid					1827:1830	docosanoic acid	1816:1830	docosanoic acid	1816:1830	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	11	18	theme	fatty	1747:1751	arg1	acids					1753:1757	unsaturated fatty acids	1735:1757	unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid	1735:1830	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	11	18	theme	fatty	1747:1751	arg1	acid					1777:1780	stearic acid	1769:1780	stearic acid	1769:1780	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	11	18	theme	fatty	1747:1751	arg1	acid					1793:1796	arachidic acid	1783:1796	arachidic acid	1783:1796	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	11	18	theme	fatty	1747:1751	arg1	acid					1806:1809	erucic acid	1799:1809	erucic acid	1799:1809	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	9	19	theme	genes	1192:1196	arg1	permeability					1109:1120	Increased intestinal permeability	1088:1120	Increased intestinal permeability	1088:1120	Increased intestinal permeability and decreased expressions of intestinal epithelial barrier associated genes were observed due to ketogenic diet administration.
34542110	9	19	theme	genes	1192:1196	arg1	expressions					1136:1146	decreased expressions	1126:1146	decreased expressions of intestinal epithelial barrier associated genes	1126:1196	Increased intestinal permeability and decreased expressions of intestinal epithelial barrier associated genes were observed due to ketogenic diet administration.
34542110	1	20	theme	bowel	137:141	arg1	IBD					152:154	IBD	152:154	IBD	152:154	Inflammatory bowel disease (IBD) is an idiopathic inflammatory disease with a high incidence.
34542110	1	20	theme	bowel	137:141	arg1	disease					187:193	an idiopathic inflammatory disease	160:193	an idiopathic inflammatory disease with a high incidence	160:215	Inflammatory bowel disease (IBD) is an idiopathic inflammatory disease with a high incidence.
34542110	1	20	theme	bowel	137:141	arg1	disease					143:149	Inflammatory bowel disease	124:149	Inflammatory bowel disease (IBD)	124:155	Inflammatory bowel disease (IBD) is an idiopathic inflammatory disease with a high incidence.
34542110	5	21	theme	C57BL/6	510:516	arg1	mice					518:521	C57BL/6 mice	510:521	C57BL/6 mice	510:521	C57BL/6 mice were given a ketogenic diet or a control diet for a month and IBD was induced by 2% DSS in drinking water in the last week.
34542110	0	22	theme	gut	73:75	arg1	microbiota					77:86	gut microbiota	73:86	gut microbiota	73:86	Ketogenic diet aggravates colitis, impairs intestinal barrier and alters gut microbiota and metabolism in DSS-induced mice.
34542110	7	23	theme	length	882:887	arg1	shortening					889:898	colon length shortening	876:898	colon length shortening	876:898	Ketogenic diet substantially worsened colitis, in terms of higher body weight loss, DAI scores and histological scores as well as colon length shortening.
34542110	12	24	from	colitis	1898:1904	arg1	mice					1909:1912	mice	1909:1912	mice	1909:1912	These results suggest that ketogenic diet aggravates DSS-induced colitis in mice by increasing intestinal and systemic inflammation, and disrupting the intestinal barrier, which results from modulated gut microbiota and metabolism.
34542110	11	25	theme	Ketogenic	1494:1502	arg1	diet					1504:1507	Ketogenic diet	1494:1507	Ketogenic diet	1494:1507	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	9	26	theme	diet	1229:1232	arg1	administration					1234:1247	ketogenic diet administration	1219:1247	ketogenic diet administration	1219:1247	Increased intestinal permeability and decreased expressions of intestinal epithelial barrier associated genes were observed due to ketogenic diet administration.
34542110	3	27	theme	high	340:343	arg1	proportion					345:354	a high proportion	338:354	a high proportion of fat and low carbohydrates	338:383	Recently, ketogenic diet which consists of a high proportion of fat and low carbohydrates has gained great popularity.
34542110	10	28	theme	beneficial	1449:1458	arg1	taxa					1460:1463	potential beneficial taxa	1439:1463	potential beneficial taxa such as Erysipelotrichaceae	1439:1491	Pretreatment with ketogenic diet alters the bacterial abundance, increasing pathogenic taxa such as Proteobacteria, Enterobacteriaceae, Helicobacter and Escherichia-Shigella and decreasing potential beneficial taxa such as Erysipelotrichaceae.
34542110	10	28	theme	beneficial	1449:1458	arg1	Erysipelotrichaceae					1473:1491	Erysipelotrichaceae	1473:1491	Erysipelotrichaceae	1473:1491	Pretreatment with ketogenic diet alters the bacterial abundance, increasing pathogenic taxa such as Proteobacteria, Enterobacteriaceae, Helicobacter and Escherichia-Shigella and decreasing potential beneficial taxa such as Erysipelotrichaceae.
34542110	3	29	theme	carbohydrates	371:383	arg1	proportion					345:354	a high proportion	338:354	a high proportion of fat and low carbohydrates	338:383	Recently, ketogenic diet which consists of a high proportion of fat and low carbohydrates has gained great popularity.
34542110	4	30	theme	diet	468:471	arg1	effect					448:453	the effect	444:453	the effect of ketogenic diet on IBD and its potential mechanisms	444:507	Our study is aimed to explore the effect of ketogenic diet on IBD and its potential mechanisms.
34542110	0	31	theme	Ketogenic	0:8	arg1	diet					10:13	Ketogenic diet	0:13	Ketogenic diet	0:13	Ketogenic diet aggravates colitis, impairs intestinal barrier and alters gut microbiota and metabolism in DSS-induced mice.
34542110	7	32	theme	histological	845:856	arg1	scores					858:863	histological scores	845:863	histological scores	845:863	Ketogenic diet substantially worsened colitis, in terms of higher body weight loss, DAI scores and histological scores as well as colon length shortening.
34542110	9	33	theme	Increased	1088:1096	arg1	permeability					1109:1120	Increased intestinal permeability	1088:1120	Increased intestinal permeability	1088:1120	Increased intestinal permeability and decreased expressions of intestinal epithelial barrier associated genes were observed due to ketogenic diet administration.
34542110	12	34	theme	ketogenic	1860:1868	arg1	diet					1870:1873	ketogenic diet	1860:1873	ketogenic diet	1860:1873	These results suggest that ketogenic diet aggravates DSS-induced colitis in mice by increasing intestinal and systemic inflammation, and disrupting the intestinal barrier, which results from modulated gut microbiota and metabolism.
34542110	0	35	theme	intestinal	43:52	arg1	barrier					54:60	intestinal barrier	43:60	intestinal barrier	43:60	Ketogenic diet aggravates colitis, impairs intestinal barrier and alters gut microbiota and metabolism in DSS-induced mice.
34542110	11	36	dep	modified	1514:1521	arg1	decreasing					1676:1685	decreasing	1676:1685	decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid	1676:1830	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	11	36	dep	modified	1514:1521	arg1	increasing					1539:1548	increasing	1539:1548	increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid	1539:1669	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	7	37	theme	DAI	830:832	arg1	scores					834:839	DAI scores	830:839	DAI scores	830:839	Ketogenic diet substantially worsened colitis, in terms of higher body weight loss, DAI scores and histological scores as well as colon length shortening.
34542110	11	38	theme	cholic	1638:1643	arg1	metabolites					1550:1560	metabolites	1550:1560	metabolites in the bile secretion such as ouabain	1550:1598	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	11	38	theme	cholic	1638:1643	arg1	ouabain					1592:1598	ouabain	1592:1598	ouabain	1592:1598	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	11	38	theme	cholic	1638:1643	arg1	acid					1645:1648	cholic acid	1638:1648	cholic acid	1638:1648	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	6	39	theme	Gut	647:649	arg1	histology					651:659	Gut histology	647:659	Gut histology	647:659	Gut histology, inflammatory cytokines and chemokines, gut microbiota and metabolism were assessed.
34542110	7	40	theme	loss	824:827	arg1	terms					796:800	terms	796:800	terms of higher body weight loss, DAI scores and histological scores as well as colon length shortening	796:898	Ketogenic diet substantially worsened colitis, in terms of higher body weight loss, DAI scores and histological scores as well as colon length shortening.
34542110	12	41	theme	gut	2034:2036	arg1	microbiota					2038:2047	modulated gut microbiota	2024:2047	modulated gut microbiota	2024:2047	These results suggest that ketogenic diet aggravates DSS-induced colitis in mice by increasing intestinal and systemic inflammation, and disrupting the intestinal barrier, which results from modulated gut microbiota and metabolism.
34542110	5	42	theme	%	605:605	arg1	DSS					607:609	2% DSS	604:609	2% DSS in drinking water in the last week	604:644	C57BL/6 mice were given a ketogenic diet or a control diet for a month and IBD was induced by 2% DSS in drinking water in the last week.
34542110	7	43	theme	body	812:815	arg1	loss					824:827	higher body weight loss	805:827	higher body weight loss	805:827	Ketogenic diet substantially worsened colitis, in terms of higher body weight loss, DAI scores and histological scores as well as colon length shortening.
34542110	8	44	theme	serum	911:915	arg1	Levels					901:906	Levels	901:906	Levels of serum and colon inflammatory cytokines and chemokines (IL-1α, IL-6, TNF-α, IL-17, GM-CSF and IL-10)	901:1009	Levels of serum and colon inflammatory cytokines and chemokines (IL-1α, IL-6, TNF-α, IL-17, GM-CSF and IL-10) were significantly up-regulated in mice treated with ketogenic diet and DSS.
34542110	11	45	theme	gut	1523:1525	arg1	metabolism					1527:1536	gut metabolism	1523:1536	gut metabolism	1523:1536	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	8	46	theme	ketogenic	1064:1072	arg1	diet					1074:1077	ketogenic diet	1064:1077	ketogenic diet	1064:1077	Levels of serum and colon inflammatory cytokines and chemokines (IL-1α, IL-6, TNF-α, IL-17, GM-CSF and IL-10) were significantly up-regulated in mice treated with ketogenic diet and DSS.
34542110	8	47	theme	colon	921:925	arg1	IL-10					1004:1008	IL-10	1004:1008	IL-10	1004:1008	Levels of serum and colon inflammatory cytokines and chemokines (IL-1α, IL-6, TNF-α, IL-17, GM-CSF and IL-10) were significantly up-regulated in mice treated with ketogenic diet and DSS.
34542110	8	47	theme	colon	921:925	arg1	IL-1α					966:970	IL-1α	966:970	IL-1α	966:970	Levels of serum and colon inflammatory cytokines and chemokines (IL-1α, IL-6, TNF-α, IL-17, GM-CSF and IL-10) were significantly up-regulated in mice treated with ketogenic diet and DSS.
34542110	8	47	theme	colon	921:925	arg1	IL-17					986:990	IL-17	986:990	IL-17	986:990	Levels of serum and colon inflammatory cytokines and chemokines (IL-1α, IL-6, TNF-α, IL-17, GM-CSF and IL-10) were significantly up-regulated in mice treated with ketogenic diet and DSS.
34542110	8	47	theme	colon	921:925	arg1	GM-CSF					993:998	GM-CSF	993:998	GM-CSF	993:998	Levels of serum and colon inflammatory cytokines and chemokines (IL-1α, IL-6, TNF-α, IL-17, GM-CSF and IL-10) were significantly up-regulated in mice treated with ketogenic diet and DSS.
34542110	8	47	theme	colon	921:925	arg1	cytokines					940:948	colon inflammatory cytokines	921:948	colon inflammatory cytokines	921:948	Levels of serum and colon inflammatory cytokines and chemokines (IL-1α, IL-6, TNF-α, IL-17, GM-CSF and IL-10) were significantly up-regulated in mice treated with ketogenic diet and DSS.
34542110	8	47	theme	colon	921:925	arg1	IL-6					973:976	IL-6	973:976	IL-6	973:976	Levels of serum and colon inflammatory cytokines and chemokines (IL-1α, IL-6, TNF-α, IL-17, GM-CSF and IL-10) were significantly up-regulated in mice treated with ketogenic diet and DSS.
34542110	8	47	theme	colon	921:925	arg1	TNF-α					979:983	TNF-α	979:983	TNF-α	979:983	Levels of serum and colon inflammatory cytokines and chemokines (IL-1α, IL-6, TNF-α, IL-17, GM-CSF and IL-10) were significantly up-regulated in mice treated with ketogenic diet and DSS.
34542110	1	48	theme	idiopathic	163:172	arg1	disease					143:149	Inflammatory bowel disease	124:149	Inflammatory bowel disease (IBD)	124:155	Inflammatory bowel disease (IBD) is an idiopathic inflammatory disease with a high incidence.
34542110	1	48	theme	idiopathic	163:172	arg1	disease					187:193	an idiopathic inflammatory disease	160:193	an idiopathic inflammatory disease with a high incidence	160:215	Inflammatory bowel disease (IBD) is an idiopathic inflammatory disease with a high incidence.
34542110	8	49	theme	cytokines	940:948	arg1	Levels					901:906	Levels	901:906	Levels of serum and colon inflammatory cytokines and chemokines (IL-1α, IL-6, TNF-α, IL-17, GM-CSF and IL-10)	901:1009	Levels of serum and colon inflammatory cytokines and chemokines (IL-1α, IL-6, TNF-α, IL-17, GM-CSF and IL-10) were significantly up-regulated in mice treated with ketogenic diet and DSS.
34542110	11	50	theme	erucic	1799:1804	arg1	acid					1806:1809	erucic acid	1799:1809	erucic acid	1799:1809	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	4	51	from	effect	448:453	arg1	mechanisms					498:507	its potential mechanisms	484:507	its potential mechanisms	484:507	Our study is aimed to explore the effect of ketogenic diet on IBD and its potential mechanisms.
34542110	4	51	from	effect	448:453	arg1	IBD					476:478	IBD	476:478	IBD	476:478	Our study is aimed to explore the effect of ketogenic diet on IBD and its potential mechanisms.
34542110	9	52	theme	decreased	1126:1134	arg1	expressions					1136:1146	decreased expressions	1126:1146	decreased expressions of intestinal epithelial barrier associated genes	1126:1196	Increased intestinal permeability and decreased expressions of intestinal epithelial barrier associated genes were observed due to ketogenic diet administration.
34542110	11	53	theme	bile	1569:1572	arg1	secretion					1574:1582	the bile secretion	1565:1582	the bile secretion	1565:1582	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	0	54	from	microbiota	77:86	arg1	mice					118:121	DSS-induced mice	106:121	DSS-induced mice	106:121	Ketogenic diet aggravates colitis, impairs intestinal barrier and alters gut microbiota and metabolism in DSS-induced mice.
34542110	11	55	theme	docosanoic	1816:1825	arg1	acid					1827:1830	docosanoic acid	1816:1830	docosanoic acid	1816:1830	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	9	56	theme	epithelial	1162:1171	arg1	barrier					1173:1179	intestinal epithelial barrier	1151:1179	intestinal epithelial barrier associated genes	1151:1196	Increased intestinal permeability and decreased expressions of intestinal epithelial barrier associated genes were observed due to ketogenic diet administration.
34542110	6	57	theme	gut	701:703	arg1	microbiota					705:714	gut microbiota	701:714	gut microbiota	701:714	Gut histology, inflammatory cytokines and chemokines, gut microbiota and metabolism were assessed.
34542110	9	58	theme	associated	1181:1190	arg1	genes					1192:1196	intestinal epithelial barrier associated genes	1151:1196	intestinal epithelial barrier associated genes	1151:1196	Increased intestinal permeability and decreased expressions of intestinal epithelial barrier associated genes were observed due to ketogenic diet administration.
34542110	5	59	theme	ketogenic	536:544	arg1	diet					546:549	a ketogenic diet	534:549	a ketogenic diet	534:549	C57BL/6 mice were given a ketogenic diet or a control diet for a month and IBD was induced by 2% DSS in drinking water in the last week.
34542110	1	60	theme	Inflammatory	124:135	arg1	IBD					152:154	IBD	152:154	IBD	152:154	Inflammatory bowel disease (IBD) is an idiopathic inflammatory disease with a high incidence.
34542110	1	60	theme	Inflammatory	124:135	arg1	disease					187:193	an idiopathic inflammatory disease	160:193	an idiopathic inflammatory disease with a high incidence	160:215	Inflammatory bowel disease (IBD) is an idiopathic inflammatory disease with a high incidence.
34542110	1	60	theme	Inflammatory	124:135	arg1	disease					143:149	Inflammatory bowel disease	124:149	Inflammatory bowel disease (IBD)	124:155	Inflammatory bowel disease (IBD) is an idiopathic inflammatory disease with a high incidence.
34542110	0	61	from	metabolism	92:101	arg1	mice					118:121	DSS-induced mice	106:121	DSS-induced mice	106:121	Ketogenic diet aggravates colitis, impairs intestinal barrier and alters gut microbiota and metabolism in DSS-induced mice.
34542110	11	62	theme	acids	1753:1757	arg1	biosynthesis					1719:1730	the biosynthesis	1715:1730	the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid	1715:1830	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	2	63	theme	dietary	245:251	arg1	composition					253:263	dietary composition	245:263	dietary composition	245:263	Multiple factors including dietary composition contribute to its occurrence.
34542110	11	64	theme	unsaturated	1735:1745	arg1	acid					1827:1830	docosanoic acid	1816:1830	docosanoic acid	1816:1830	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	11	64	theme	unsaturated	1735:1745	arg1	acids					1753:1757	unsaturated fatty acids	1735:1757	unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid	1735:1830	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	11	64	theme	unsaturated	1735:1745	arg1	acid					1777:1780	stearic acid	1769:1780	stearic acid	1769:1780	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	11	64	theme	unsaturated	1735:1745	arg1	acid					1793:1796	arachidic acid	1783:1796	arachidic acid	1783:1796	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	11	64	theme	unsaturated	1735:1745	arg1	acid					1806:1809	erucic acid	1799:1809	erucic acid	1799:1809	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	4	65	theme	potential	488:496	arg1	mechanisms					498:507	its potential mechanisms	484:507	its potential mechanisms	484:507	Our study is aimed to explore the effect of ketogenic diet on IBD and its potential mechanisms.
34542110	9	66	theme	ketogenic	1219:1227	arg1	administration					1234:1247	ketogenic diet administration	1219:1247	ketogenic diet administration	1219:1247	Increased intestinal permeability and decreased expressions of intestinal epithelial barrier associated genes were observed due to ketogenic diet administration.
34542110	12	67	theme	intestinal	1928:1937	arg1	inflammation					1952:1963	intestinal and systemic inflammation	1928:1963	inflammation	1952:1963	These results suggest that ketogenic diet aggravates DSS-induced colitis in mice by increasing intestinal and systemic inflammation, and disrupting the intestinal barrier, which results from modulated gut microbiota and metabolism.
34542110	7	68	theme	shortening	889:898	arg1	terms					796:800	terms	796:800	terms of higher body weight loss, DAI scores and histological scores as well as colon length shortening	796:898	Ketogenic diet substantially worsened colitis, in terms of higher body weight loss, DAI scores and histological scores as well as colon length shortening.
34542110	3	69	theme	fat	359:361	arg1	carbohydrates					371:383	fat and low carbohydrates	359:383	fat and low carbohydrates	359:383	Recently, ketogenic diet which consists of a high proportion of fat and low carbohydrates has gained great popularity.
34542110	4	70	theme	ketogenic	458:466	arg1	diet					468:471	ketogenic diet	458:471	ketogenic diet	458:471	Our study is aimed to explore the effect of ketogenic diet on IBD and its potential mechanisms.
34542110	8	71	dep	cytokines	940:948	arg1	IL-10					1004:1008	IL-10	1004:1008	IL-10	1004:1008	Levels of serum and colon inflammatory cytokines and chemokines (IL-1α, IL-6, TNF-α, IL-17, GM-CSF and IL-10) were significantly up-regulated in mice treated with ketogenic diet and DSS.
34542110	8	71	dep	cytokines	940:948	arg1	IL-1α					966:970	IL-1α	966:970	IL-1α	966:970	Levels of serum and colon inflammatory cytokines and chemokines (IL-1α, IL-6, TNF-α, IL-17, GM-CSF and IL-10) were significantly up-regulated in mice treated with ketogenic diet and DSS.
34542110	8	71	dep	cytokines	940:948	arg1	IL-17					986:990	IL-17	986:990	IL-17	986:990	Levels of serum and colon inflammatory cytokines and chemokines (IL-1α, IL-6, TNF-α, IL-17, GM-CSF and IL-10) were significantly up-regulated in mice treated with ketogenic diet and DSS.
34542110	8	71	dep	cytokines	940:948	arg1	GM-CSF					993:998	GM-CSF	993:998	GM-CSF	993:998	Levels of serum and colon inflammatory cytokines and chemokines (IL-1α, IL-6, TNF-α, IL-17, GM-CSF and IL-10) were significantly up-regulated in mice treated with ketogenic diet and DSS.
34542110	8	71	dep	cytokines	940:948	arg1	cytokines					940:948	colon inflammatory cytokines	921:948	colon inflammatory cytokines	921:948	Levels of serum and colon inflammatory cytokines and chemokines (IL-1α, IL-6, TNF-α, IL-17, GM-CSF and IL-10) were significantly up-regulated in mice treated with ketogenic diet and DSS.
34542110	8	71	dep	cytokines	940:948	arg1	IL-6					973:976	IL-6	973:976	IL-6	973:976	Levels of serum and colon inflammatory cytokines and chemokines (IL-1α, IL-6, TNF-α, IL-17, GM-CSF and IL-10) were significantly up-regulated in mice treated with ketogenic diet and DSS.
34542110	8	71	dep	cytokines	940:948	arg1	TNF-α					979:983	TNF-α	979:983	TNF-α	979:983	Levels of serum and colon inflammatory cytokines and chemokines (IL-1α, IL-6, TNF-α, IL-17, GM-CSF and IL-10) were significantly up-regulated in mice treated with ketogenic diet and DSS.
34542110	11	72	theme	stearic	1769:1775	arg1	acid					1777:1780	stearic acid	1769:1780	stearic acid	1769:1780	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	0	73	theme	DSS-induced	106:116	arg1	mice					118:121	DSS-induced mice	106:121	DSS-induced mice	106:121	Ketogenic diet aggravates colitis, impairs intestinal barrier and alters gut microbiota and metabolism in DSS-induced mice.
34542110	12	74	theme	DSS-induced	1886:1896	arg1	colitis					1898:1904	DSS-induced colitis	1886:1904	DSS-induced colitis in mice	1886:1912	These results suggest that ketogenic diet aggravates DSS-induced colitis in mice by increasing intestinal and systemic inflammation, and disrupting the intestinal barrier, which results from modulated gut microbiota and metabolism.
34542110	7	75	theme	colon	876:880	arg1	shortening					889:898	colon length shortening	876:898	colon length shortening	876:898	Ketogenic diet substantially worsened colitis, in terms of higher body weight loss, DAI scores and histological scores as well as colon length shortening.
34542110	10	76	theme	potential	1439:1447	arg1	taxa					1460:1463	potential beneficial taxa	1439:1463	potential beneficial taxa such as Erysipelotrichaceae	1439:1491	Pretreatment with ketogenic diet alters the bacterial abundance, increasing pathogenic taxa such as Proteobacteria, Enterobacteriaceae, Helicobacter and Escherichia-Shigella and decreasing potential beneficial taxa such as Erysipelotrichaceae.
34542110	10	76	theme	potential	1439:1447	arg1	Erysipelotrichaceae					1473:1491	Erysipelotrichaceae	1473:1491	Erysipelotrichaceae	1473:1491	Pretreatment with ketogenic diet alters the bacterial abundance, increasing pathogenic taxa such as Proteobacteria, Enterobacteriaceae, Helicobacter and Escherichia-Shigella and decreasing potential beneficial taxa such as Erysipelotrichaceae.
34542110	10	77	with	Pretreatment	1250:1261	arg1	diet					1278:1281	ketogenic diet	1268:1281	ketogenic diet	1268:1281	Pretreatment with ketogenic diet alters the bacterial abundance, increasing pathogenic taxa such as Proteobacteria, Enterobacteriaceae, Helicobacter and Escherichia-Shigella and decreasing potential beneficial taxa such as Erysipelotrichaceae.
34542110	7	78	theme	Ketogenic	746:754	arg1	diet					756:759	Ketogenic diet	746:759	Ketogenic diet	746:759	Ketogenic diet substantially worsened colitis, in terms of higher body weight loss, DAI scores and histological scores as well as colon length shortening.
34542110	7	79	theme	scores	858:863	arg1	terms					796:800	terms	796:800	terms of higher body weight loss, DAI scores and histological scores as well as colon length shortening	796:898	Ketogenic diet substantially worsened colitis, in terms of higher body weight loss, DAI scores and histological scores as well as colon length shortening.
34542110	3	80	theme	low	367:369	arg1	carbohydrates					371:383	fat and low carbohydrates	359:383	fat and low carbohydrates	359:383	Recently, ketogenic diet which consists of a high proportion of fat and low carbohydrates has gained great popularity.
34542110	1	81	with	disease	187:193	arg1	incidence					207:215	a high incidence	200:215	a high incidence	200:215	Inflammatory bowel disease (IBD) is an idiopathic inflammatory disease with a high incidence.
34542110	3	82	theme	great	396:400	arg1	popularity					402:411	great popularity	396:411	great popularity	396:411	Recently, ketogenic diet which consists of a high proportion of fat and low carbohydrates has gained great popularity.
34542110	6	83	theme	inflammatory	662:673	arg1	cytokines					675:683	cytokines	675:683	cytokines	675:683	Gut histology, inflammatory cytokines and chemokines, gut microbiota and metabolism were assessed.
34542110	10	84	theme	ketogenic	1268:1276	arg1	diet					1278:1281	ketogenic diet	1268:1281	ketogenic diet	1268:1281	Pretreatment with ketogenic diet alters the bacterial abundance, increasing pathogenic taxa such as Proteobacteria, Enterobacteriaceae, Helicobacter and Escherichia-Shigella and decreasing potential beneficial taxa such as Erysipelotrichaceae.
34542110	7	85	theme	scores	834:839	arg1	terms					796:800	terms	796:800	terms of higher body weight loss, DAI scores and histological scores as well as colon length shortening	796:898	Ketogenic diet substantially worsened colitis, in terms of higher body weight loss, DAI scores and histological scores as well as colon length shortening.
34542110	11	86	theme	taurochenodeoxycholic	1601:1621	arg1	metabolites					1550:1560	metabolites	1550:1560	metabolites in the bile secretion such as ouabain	1550:1598	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	11	86	theme	taurochenodeoxycholic	1601:1621	arg1	ouabain					1592:1598	ouabain	1592:1598	ouabain	1592:1598	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	11	86	theme	taurochenodeoxycholic	1601:1621	arg1	acid					1623:1626	taurochenodeoxycholic acid	1601:1626	taurochenodeoxycholic acid	1601:1626	Ketogenic diet also modified gut metabolism, increasing metabolites in the bile secretion such as ouabain, taurochenodeoxycholic acid, quinine, cholic acid and glycocholic acid, and decreasing metabolites associated with the biosynthesis of unsaturated fatty acids including stearic acid, arachidic acid, erucic acid, and docosanoic acid.
34542110	12	87	theme	modulated	2024:2032	arg1	microbiota					2038:2047	modulated gut microbiota	2024:2047	modulated gut microbiota	2024:2047	These results suggest that ketogenic diet aggravates DSS-induced colitis in mice by increasing intestinal and systemic inflammation, and disrupting the intestinal barrier, which results from modulated gut microbiota and metabolism.
34542110	10	88	theme	bacterial	1294:1302	arg1	abundance					1304:1312	the bacterial abundance	1290:1312	the bacterial abundance	1290:1312	Pretreatment with ketogenic diet alters the bacterial abundance, increasing pathogenic taxa such as Proteobacteria, Enterobacteriaceae, Helicobacter and Escherichia-Shigella and decreasing potential beneficial taxa such as Erysipelotrichaceae.
34542110	7	89	theme	weight	817:822	arg1	loss					824:827	higher body weight loss	805:827	higher body weight loss	805:827	Ketogenic diet substantially worsened colitis, in terms of higher body weight loss, DAI scores and histological scores as well as colon length shortening.
33289624	9	0	theme	sugar	1185:1189	arg1	components					1191:1200	sugar components	1185:1200	sugar components of the cell wall peptidoglycan	1185:1231	The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan.
33289624	9	0	theme	sugar	1185:1189	arg1	ʟ-alanine					1097:1105	ʟ-alanine	1097:1105	ʟ-alanine	1097:1105	The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan.
33289624	9	0	theme	sugar	1185:1189	arg1	ʟ-lysine					1112:1119	ʟ-lysine	1112:1119	ʟ-lysine	1112:1119	The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan.
33289624	9	0	theme	sugar	1185:1189	arg1	acid					1091:1094	ʟʟ-diaminopimelic acid	1073:1094	ʟʟ-diaminopimelic acid	1073:1094	The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan.
33289624	10	1	theme	novel	1356:1360	arg1	species					1362:1368	a novel species	1354:1368	a novel species within the genus Tessaracoccus	1354:1399	The results of phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses suggested that strain HDW20T represents a novel species within the genus Tessaracoccus.
33289624	9	2	theme	peptidoglycan	1219:1231	arg1	components					1191:1200	sugar components	1185:1200	sugar components of the cell wall peptidoglycan	1185:1231	The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan.
33289624	9	2	theme	peptidoglycan	1219:1231	arg1	ʟ-alanine					1097:1105	ʟ-alanine	1097:1105	ʟ-alanine	1097:1105	The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan.
33289624	9	2	theme	peptidoglycan	1219:1231	arg1	ʟ-lysine					1112:1119	ʟ-lysine	1112:1119	ʟ-lysine	1112:1119	The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan.
33289624	9	2	theme	peptidoglycan	1219:1231	arg1	acid					1091:1094	ʟʟ-diaminopimelic acid	1073:1094	ʟʟ-diaminopimelic acid	1073:1094	The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan.
33289624	11	3	theme	coleopterorum	1436:1448	arg1	sp					1450:1451	the name Tessaracoccus coleopterorum sp	1413:1451	the name Tessaracoccus coleopterorum sp	1413:1451	We propose the name Tessaracoccus coleopterorum sp.
33289624	4	4	theme	%	809:809	arg1	value					795:799	a highest average nucleotide identity (ANI) value	751:799	a highest average nucleotide identity (ANI) value of 80.6 %	751:809	Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus Tessaracoccus in the phylum Actinobacteria and was closely related to T. flavescens SST-39T, T. defluvii JCM 17540T, and T. aquimaris NSG39T, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %.
33289624	4	4	theme	%	809:809	arg1	%					809:809	80.6 %	804:809	80.6 %	804:809	Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus Tessaracoccus in the phylum Actinobacteria and was closely related to T. flavescens SST-39T, T. defluvii JCM 17540T, and T. aquimaris NSG39T, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %.
33289624	4	4	theme	%	809:809	arg1	%					745:745	98.5 %	740:745	98.5 %	740:745	Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus Tessaracoccus in the phylum Actinobacteria and was closely related to T. flavescens SST-39T, T. defluvii JCM 17540T, and T. aquimaris NSG39T, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %.
33289624	10	5	theme	genotypic	1295:1303	arg1	analyses					1305:1312	phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses	1249:1312	phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses	1249:1312	The results of phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses suggested that strain HDW20T represents a novel species within the genus Tessaracoccus.
33289624	5	6	dep	C18 	848:851	arg1	 0					877:878	 0	877:878	C18 : 1 ω9c and anteiso-C15 : 0	848:878	The major cellular fatty acids were C18 : 1 ω9c and anteiso-C15 : 0.
33289624	5	6	dep	C18 	848:851	arg1	anteiso-C15 					864:875	anteiso-C15 	864:875	anteiso-C15 	864:875	The major cellular fatty acids were C18 : 1 ω9c and anteiso-C15 : 0.
33289624	5	6	dep	C18 	848:851	arg1	ω9c					856:858	 1 ω9c	853:858	 1 ω9c	853:858	The major cellular fatty acids were C18 : 1 ω9c and anteiso-C15 : 0.
33289624	4	7	theme	phylum	565:570	arg1	Actinobacteria					572:585	the phylum Actinobacteria	561:585	the phylum Actinobacteria	561:585	Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus Tessaracoccus in the phylum Actinobacteria and was closely related to T. flavescens SST-39T, T. defluvii JCM 17540T, and T. aquimaris NSG39T, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %.
33289624	2	8	used	used	153:156	arg2	approach					140:147	A polyphasic taxonomic approach	117:147	A polyphasic taxonomic approach	117:147	A polyphasic taxonomic approach was used to characterize a novel bacterium, designated as strain HDW20T, isolated from the intestine of the dark diving beetle Hydrophilus acuminatus.
33289624	10	9	theme	phenotypic	1263:1272	arg1	analyses					1305:1312	phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses	1249:1312	phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses	1249:1312	The results of phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses suggested that strain HDW20T represents a novel species within the genus Tessaracoccus.
33289624	2	10	theme	diving	262:267	arg1	beetle					269:274	the dark diving beetle	253:274	the dark diving beetle	253:274	A polyphasic taxonomic approach was used to characterize a novel bacterium, designated as strain HDW20T, isolated from the intestine of the dark diving beetle Hydrophilus acuminatus.
33289624	4	11	theme	gene	712:715	arg1	similarity					726:735	the highest 16S rRNA gene sequence similarity	691:735	the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %	691:809	Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus Tessaracoccus in the phylum Actinobacteria and was closely related to T. flavescens SST-39T, T. defluvii JCM 17540T, and T. aquimaris NSG39T, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %.
33289624	4	12	theme	rRNA	707:710	arg1	similarity					726:735	the highest 16S rRNA gene sequence similarity	691:735	the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %	691:809	Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus Tessaracoccus in the phylum Actinobacteria and was closely related to T. flavescens SST-39T, T. defluvii JCM 17540T, and T. aquimaris NSG39T, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %.
33289624	13	13	theme	=KACC	1486:1490	arg1	33674T					1515:1520	=KACC 21348T=KCTC 49324T=JCM 33674T	1486:1520	=KACC 21348T=KCTC 49324T=JCM 33674T	1486:1520	The type strain is HDW20T (=KACC 21348T=KCTC 49324T=JCM 33674T).
33289624	13	13	theme	=KACC	1486:1490	arg1	HDW20T					1478:1483	HDW20T	1478:1483	HDW20T (=KACC 21348T=KCTC 49324T=JCM 33674T)	1478:1521	The type strain is HDW20T (=KACC 21348T=KCTC 49324T=JCM 33674T).
33289624	2	14	theme	dark	257:260	arg1	beetle					269:274	the dark diving beetle	253:274	the dark diving beetle	253:274	A polyphasic taxonomic approach was used to characterize a novel bacterium, designated as strain HDW20T, isolated from the intestine of the dark diving beetle Hydrophilus acuminatus.
33289624	1	15	attach	isolated	38:45	arg2	nov.					32:35	nov.	32:35	nov.	32:35	nov., isolated from the intestine of the dark diving beetle, Hydrophilus acuminatus.
33289624	1	15	attach	isolated	38:45	arg1	intestine					56:64	the intestine	52:64	the intestine of the dark diving beetle	52:90	nov., isolated from the intestine of the dark diving beetle, Hydrophilus acuminatus.
33289624	4	16	theme	gene	462:465	arg1	sequences					467:475	16S rRNA gene sequences	453:475	16S rRNA gene sequences	453:475	Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus Tessaracoccus in the phylum Actinobacteria and was closely related to T. flavescens SST-39T, T. defluvii JCM 17540T, and T. aquimaris NSG39T, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %.
33289624	4	17	theme	value	795:799	arg1	similarity					726:735	the highest 16S rRNA gene sequence similarity	691:735	the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %	691:809	Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus Tessaracoccus in the phylum Actinobacteria and was closely related to T. flavescens SST-39T, T. defluvii JCM 17540T, and T. aquimaris NSG39T, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %.
33289624	10	18	theme	chemotaxonomic	1275:1288	arg1	analyses					1305:1312	phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses	1249:1312	phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses	1249:1312	The results of phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses suggested that strain HDW20T represents a novel species within the genus Tessaracoccus.
33289624	1	19	dep	Hydrophilus	93:103	arg1	acuminatus					105:114	Hydrophilus acuminatus	93:114	Hydrophilus acuminatus	93:114	nov., isolated from the intestine of the dark diving beetle, Hydrophilus acuminatus.
33289624	1	19	dep	Hydrophilus	93:103	arg1	nov.					32:35	nov.	32:35	nov.	32:35	nov., isolated from the intestine of the dark diving beetle, Hydrophilus acuminatus.
33289624	9	20	theme	wall	1214:1217	arg1	peptidoglycan					1219:1231	the cell wall peptidoglycan	1205:1231	the cell wall peptidoglycan	1205:1231	The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan.
33289624	4	21	theme	JCM	649:651	arg1	17540T					653:658	JCM 17540T	649:658	T. defluvii JCM 17540T	637:658	Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus Tessaracoccus in the phylum Actinobacteria and was closely related to T. flavescens SST-39T, T. defluvii JCM 17540T, and T. aquimaris NSG39T, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %.
33289624	2	22	theme	taxonomic	130:138	arg1	approach					140:147	A polyphasic taxonomic approach	117:147	A polyphasic taxonomic approach	117:147	A polyphasic taxonomic approach was used to characterize a novel bacterium, designated as strain HDW20T, isolated from the intestine of the dark diving beetle Hydrophilus acuminatus.
33289624	6	23	theme	main	885:888	arg1	MK-9					914:917	MK-9	914:917	MK-9 (H4)	914:922	The main respiratory quinone was MK-9 (H4).
33289624	6	23	theme	main	885:888	arg1	quinone					902:908	The main respiratory quinone	881:908	The main respiratory quinone	881:908	The main respiratory quinone was MK-9 (H4).
33289624	10	24	theme	strain	1329:1334	arg1	HDW20T					1336:1341	strain HDW20T	1329:1341	strain HDW20T	1329:1341	The results of phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses suggested that strain HDW20T represents a novel species within the genus Tessaracoccus.
33289624	10	25	theme	phylogenetic	1249:1260	arg1	analyses					1305:1312	phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses	1249:1312	phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses	1249:1312	The results of phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses suggested that strain HDW20T represents a novel species within the genus Tessaracoccus.
33289624	13	26	theme	21348T=KCTC	1492:1502	arg1	33674T					1515:1520	=KACC 21348T=KCTC 49324T=JCM 33674T	1486:1520	=KACC 21348T=KCTC 49324T=JCM 33674T	1486:1520	The type strain is HDW20T (=KACC 21348T=KCTC 49324T=JCM 33674T).
33289624	13	26	theme	21348T=KCTC	1492:1502	arg1	HDW20T					1478:1483	HDW20T	1478:1483	HDW20T (=KACC 21348T=KCTC 49324T=JCM 33674T)	1478:1521	The type strain is HDW20T (=KACC 21348T=KCTC 49324T=JCM 33674T).
33289624	0	27	theme	coleopterorum	14:26	arg1	sp					28:29	Tessaracoccus coleopterorum sp	0:29	Tessaracoccus coleopterorum sp.	0:30	Tessaracoccus coleopterorum sp.
33289624	2	28	theme	polyphasic	119:128	arg1	approach					140:147	A polyphasic taxonomic approach	117:147	A polyphasic taxonomic approach	117:147	A polyphasic taxonomic approach was used to characterize a novel bacterium, designated as strain HDW20T, isolated from the intestine of the dark diving beetle Hydrophilus acuminatus.
33289624	9	29	theme	amino	1124:1128	arg1	ʟ-alanine					1097:1105	ʟ-alanine	1097:1105	ʟ-alanine	1097:1105	The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan.
33289624	9	29	theme	amino	1124:1128	arg1	ʟ-lysine					1112:1119	ʟ-lysine	1112:1119	ʟ-lysine	1112:1119	The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan.
33289624	9	29	theme	amino	1124:1128	arg1	acid					1091:1094	ʟʟ-diaminopimelic acid	1073:1094	ʟʟ-diaminopimelic acid	1073:1094	The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan.
33289624	9	29	theme	amino	1124:1128	arg1	components					1135:1144	amino acid components	1124:1144	amino acid components	1124:1144	The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan.
33289624	1	30	theme	dark	73:76	arg1	beetle					85:90	the dark diving beetle	69:90	the dark diving beetle	69:90	nov., isolated from the intestine of the dark diving beetle, Hydrophilus acuminatus.
33289624	9	31	theme	cell	1209:1212	arg1	peptidoglycan					1219:1231	the cell wall peptidoglycan	1205:1231	the cell wall peptidoglycan	1205:1231	The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan.
33289624	0	32	theme	Tessaracoccus	0:12	arg1	sp					28:29	Tessaracoccus coleopterorum sp	0:29	Tessaracoccus coleopterorum sp.	0:30	Tessaracoccus coleopterorum sp.
33289624	6	33	theme	respiratory	890:900	arg1	MK-9					914:917	MK-9	914:917	MK-9 (H4)	914:922	The main respiratory quinone was MK-9 (H4).
33289624	6	33	theme	respiratory	890:900	arg1	quinone					902:908	The main respiratory quinone	881:908	The main respiratory quinone	881:908	The main respiratory quinone was MK-9 (H4).
33289624	4	34	theme	Phylogenetic	422:433	arg1	analysis					435:442	Phylogenetic analysis	422:442	Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences	422:496	Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus Tessaracoccus in the phylum Actinobacteria and was closely related to T. flavescens SST-39T, T. defluvii JCM 17540T, and T. aquimaris NSG39T, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %.
33289624	9	35	theme	acid	1130:1133	arg1	ʟ-alanine					1097:1105	ʟ-alanine	1097:1105	ʟ-alanine	1097:1105	The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan.
33289624	9	35	theme	acid	1130:1133	arg1	ʟ-lysine					1112:1119	ʟ-lysine	1112:1119	ʟ-lysine	1112:1119	The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan.
33289624	9	35	theme	acid	1130:1133	arg1	acid					1091:1094	ʟʟ-diaminopimelic acid	1073:1094	ʟʟ-diaminopimelic acid	1073:1094	The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan.
33289624	9	35	theme	acid	1130:1133	arg1	components					1135:1144	amino acid components	1124:1144	amino acid components	1124:1144	The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan.
33289624	1	36	theme	diving	78:83	arg1	beetle					85:90	the dark diving beetle	69:90	the dark diving beetle	69:90	nov., isolated from the intestine of the dark diving beetle, Hydrophilus acuminatus.
33289624	5	37	theme	major	816:820	arg1	acids					837:841	The major cellular fatty acids	812:841	The major cellular fatty acids	812:841	The major cellular fatty acids were C18 : 1 ω9c and anteiso-C15 : 0.
33289624	5	37	theme	major	816:820	arg1	C18 					848:851	C18 	848:851	C18 : 1 ω9c and anteiso-C15 : 0	848:878	The major cellular fatty acids were C18 : 1 ω9c and anteiso-C15 : 0.
33289624	4	38	from	Tessaracoccus	544:556	arg1	Actinobacteria					572:585	the phylum Actinobacteria	561:585	the phylum Actinobacteria	561:585	Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus Tessaracoccus in the phylum Actinobacteria and was closely related to T. flavescens SST-39T, T. defluvii JCM 17540T, and T. aquimaris NSG39T, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %.
33289624	4	39	theme	genus	538:542	arg1	Tessaracoccus					544:556	the genus Tessaracoccus	534:556	the genus Tessaracoccus in the phylum Actinobacteria	534:585	Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus Tessaracoccus in the phylum Actinobacteria and was closely related to T. flavescens SST-39T, T. defluvii JCM 17540T, and T. aquimaris NSG39T, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %.
33289624	10	40	theme	analyses	1305:1312	arg1	results					1238:1244	The results	1234:1244	The results of phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses	1234:1312	The results of phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses suggested that strain HDW20T represents a novel species within the genus Tessaracoccus.
33289624	4	41	theme	sequence	717:724	arg1	similarity					726:735	the highest 16S rRNA gene sequence similarity	691:735	the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %	691:809	Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus Tessaracoccus in the phylum Actinobacteria and was closely related to T. flavescens SST-39T, T. defluvii JCM 17540T, and T. aquimaris NSG39T, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %.
33289624	4	42	dep	T.	637:638	arg1	17540T					653:658	JCM 17540T	649:658	T. defluvii JCM 17540T	637:658	Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus Tessaracoccus in the phylum Actinobacteria and was closely related to T. flavescens SST-39T, T. defluvii JCM 17540T, and T. aquimaris NSG39T, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %.
33289624	4	42	dep	T.	637:638	arg1	defluvii					640:647	T. defluvii JCM 17540T	637:658	T. defluvii JCM 17540T	637:658	Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus Tessaracoccus in the phylum Actinobacteria and was closely related to T. flavescens SST-39T, T. defluvii JCM 17540T, and T. aquimaris NSG39T, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %.
33289624	4	43	theme	16S	703:705	arg1	similarity					726:735	the highest 16S rRNA gene sequence similarity	691:735	the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %	691:809	Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus Tessaracoccus in the phylum Actinobacteria and was closely related to T. flavescens SST-39T, T. defluvii JCM 17540T, and T. aquimaris NSG39T, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %.
33289624	4	44	theme	%	745:745	arg1	similarity					726:735	the highest 16S rRNA gene sequence similarity	691:735	the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %	691:809	Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus Tessaracoccus in the phylum Actinobacteria and was closely related to T. flavescens SST-39T, T. defluvii JCM 17540T, and T. aquimaris NSG39T, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %.
33289624	2	45	theme	beetle	269:274	arg1	intestine					240:248	the intestine	236:248	the intestine of the dark diving beetle	236:274	A polyphasic taxonomic approach was used to characterize a novel bacterium, designated as strain HDW20T, isolated from the intestine of the dark diving beetle Hydrophilus acuminatus.
33289624	3	46	theme	pale	400:403	arg1	colonies					412:419	pale orange colonies	400:419	pale orange colonies	400:419	The isolate was Gram-stain-positive, facultatively anaerobic, non-motile, coccus-shaped, and formed pale orange colonies.
33289624	8	47	theme	DNA	1024:1026	arg1	content					1032:1038	The genomic DNA G+C content	1012:1038	The genomic DNA G+C content	1012:1038	The genomic DNA G+C content was 69.0 %.
33289624	8	47	theme	DNA	1024:1026	arg1	%					1049:1049	69.0 %	1044:1049	69.0 %	1044:1049	The genomic DNA G+C content was 69.0 %.
33289624	4	48	theme	identity	780:787	arg1	value					795:799	a highest average nucleotide identity (ANI) value	751:799	a highest average nucleotide identity (ANI) value of 80.6 %	751:809	Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus Tessaracoccus in the phylum Actinobacteria and was closely related to T. flavescens SST-39T, T. defluvii JCM 17540T, and T. aquimaris NSG39T, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %.
33289624	3	49	theme	orange	405:410	arg1	colonies					412:419	pale orange colonies	400:419	pale orange colonies	400:419	The isolate was Gram-stain-positive, facultatively anaerobic, non-motile, coccus-shaped, and formed pale orange colonies.
33289624	8	50	theme	genomic	1016:1022	arg1	content					1032:1038	The genomic DNA G+C content	1012:1038	The genomic DNA G+C content	1012:1038	The genomic DNA G+C content was 69.0 %.
33289624	8	50	theme	genomic	1016:1022	arg1	%					1049:1049	69.0 %	1044:1049	69.0 %	1044:1049	The genomic DNA G+C content was 69.0 %.
33289624	4	51	theme	rRNA	457:460	arg1	sequences					467:475	16S rRNA gene sequences	453:475	16S rRNA gene sequences	453:475	Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus Tessaracoccus in the phylum Actinobacteria and was closely related to T. flavescens SST-39T, T. defluvii JCM 17540T, and T. aquimaris NSG39T, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %.
33289624	4	52	theme	nucleotide	769:778	arg1	identity					780:787	average nucleotide identity	761:787	a highest average nucleotide identity (ANI) value of 80.6 %	751:809	Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus Tessaracoccus in the phylum Actinobacteria and was closely related to T. flavescens SST-39T, T. defluvii JCM 17540T, and T. aquimaris NSG39T, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %.
33289624	4	52	theme	nucleotide	769:778	arg1	ANI					790:792	ANI	790:792	ANI	790:792	Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus Tessaracoccus in the phylum Actinobacteria and was closely related to T. flavescens SST-39T, T. defluvii JCM 17540T, and T. aquimaris NSG39T, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %.
33289624	7	53	theme	lipid	941:945	arg1	phosphatidylglycerol					963:982	phosphatidylglycerol	963:982	phosphatidylglycerol	963:982	The major polar lipid components were phosphatidylglycerol and diphosphatidylglycerol.
33289624	7	53	theme	lipid	941:945	arg1	components					947:956	The major polar lipid components	925:956	The major polar lipid components	925:956	The major polar lipid components were phosphatidylglycerol and diphosphatidylglycerol.
33289624	4	54	theme	16S	453:455	arg1	sequences					467:475	16S rRNA gene sequences	453:475	16S rRNA gene sequences	453:475	Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus Tessaracoccus in the phylum Actinobacteria and was closely related to T. flavescens SST-39T, T. defluvii JCM 17540T, and T. aquimaris NSG39T, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %.
33289624	2	55	theme	strain	207:212	arg1	HDW20T					214:219	strain HDW20T	207:219	strain HDW20T	207:219	A polyphasic taxonomic approach was used to characterize a novel bacterium, designated as strain HDW20T, isolated from the intestine of the dark diving beetle Hydrophilus acuminatus.
33289624	4	56	theme	genome	481:486	arg1	sequences					488:496	genome sequences	481:496	genome sequences	481:496	Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus Tessaracoccus in the phylum Actinobacteria and was closely related to T. flavescens SST-39T, T. defluvii JCM 17540T, and T. aquimaris NSG39T, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %.
33289624	11	57	theme	name	1417:1420	arg1	sp					1450:1451	the name Tessaracoccus coleopterorum sp	1413:1451	the name Tessaracoccus coleopterorum sp	1413:1451	We propose the name Tessaracoccus coleopterorum sp.
33289624	8	58	theme	G+C	1028:1030	arg1	content					1032:1038	The genomic DNA G+C content	1012:1038	The genomic DNA G+C content	1012:1038	The genomic DNA G+C content was 69.0 %.
33289624	8	58	theme	G+C	1028:1030	arg1	%					1049:1049	69.0 %	1044:1049	69.0 %	1044:1049	The genomic DNA G+C content was 69.0 %.
33289624	9	59	contain	contains	1064:1071	arg2	components					1135:1144	amino acid components	1124:1144	amino acid components	1124:1144	The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan.
33289624	9	59	contain	contains	1064:1071	arg1	isolate					1056:1062	The isolate	1052:1062	The isolate	1052:1062	The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan.
33289624	9	59	contain	contains	1064:1071	arg2	components					1191:1200	sugar components	1185:1200	sugar components of the cell wall peptidoglycan	1185:1231	The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan.
33289624	9	59	contain	contains	1064:1071	arg2	ʟ-alanine					1097:1105	ʟ-alanine	1097:1105	ʟ-alanine	1097:1105	The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan.
33289624	9	59	contain	contains	1064:1071	arg2	ʟ-lysine					1112:1119	ʟ-lysine	1112:1119	ʟ-lysine	1112:1119	The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan.
33289624	9	59	contain	contains	1064:1071	arg2	acid					1091:1094	ʟʟ-diaminopimelic acid	1073:1094	ʟʟ-diaminopimelic acid	1073:1094	The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan.
33289624	7	60	theme	major	929:933	arg1	phosphatidylglycerol					963:982	phosphatidylglycerol	963:982	phosphatidylglycerol	963:982	The major polar lipid components were phosphatidylglycerol and diphosphatidylglycerol.
33289624	7	60	theme	major	929:933	arg1	components					947:956	The major polar lipid components	925:956	The major polar lipid components	925:956	The major polar lipid components were phosphatidylglycerol and diphosphatidylglycerol.
33289624	13	61	theme	type	1463:1466	arg1	HDW20T					1478:1483	HDW20T	1478:1483	HDW20T (=KACC 21348T=KCTC 49324T=JCM 33674T)	1478:1521	The type strain is HDW20T (=KACC 21348T=KCTC 49324T=JCM 33674T).
33289624	13	61	theme	type	1463:1466	arg1	strain					1468:1473	The type strain	1459:1473	The type strain	1459:1473	The type strain is HDW20T (=KACC 21348T=KCTC 49324T=JCM 33674T).
33289624	4	62	theme	highest	695:701	arg1	similarity					726:735	the highest 16S rRNA gene sequence similarity	691:735	the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %	691:809	Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus Tessaracoccus in the phylum Actinobacteria and was closely related to T. flavescens SST-39T, T. defluvii JCM 17540T, and T. aquimaris NSG39T, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %.
33289624	1	63	theme	beetle	85:90	arg1	intestine					56:64	the intestine	52:64	the intestine of the dark diving beetle	52:90	nov., isolated from the intestine of the dark diving beetle, Hydrophilus acuminatus.
33289624	7	64	theme	polar	935:939	arg1	phosphatidylglycerol					963:982	phosphatidylglycerol	963:982	phosphatidylglycerol	963:982	The major polar lipid components were phosphatidylglycerol and diphosphatidylglycerol.
33289624	7	64	theme	polar	935:939	arg1	components					947:956	The major polar lipid components	925:956	The major polar lipid components	925:956	The major polar lipid components were phosphatidylglycerol and diphosphatidylglycerol.
33289624	13	65	theme	49324T=JCM	1504:1513	arg1	33674T					1515:1520	=KACC 21348T=KCTC 49324T=JCM 33674T	1486:1520	=KACC 21348T=KCTC 49324T=JCM 33674T	1486:1520	The type strain is HDW20T (=KACC 21348T=KCTC 49324T=JCM 33674T).
33289624	13	65	theme	49324T=JCM	1504:1513	arg1	HDW20T					1478:1483	HDW20T	1478:1483	HDW20T (=KACC 21348T=KCTC 49324T=JCM 33674T)	1478:1521	The type strain is HDW20T (=KACC 21348T=KCTC 49324T=JCM 33674T).
33289624	10	66	theme	genus	1381:1385	arg1	Tessaracoccus					1387:1399	the genus Tessaracoccus	1377:1399	the genus Tessaracoccus	1377:1399	The results of phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses suggested that strain HDW20T represents a novel species within the genus Tessaracoccus.
33289624	5	67	theme	 1	853:854	arg1	ω9c					856:858	 1 ω9c	853:858	 1 ω9c	853:858	The major cellular fatty acids were C18 : 1 ω9c and anteiso-C15 : 0.
33289624	2	68	theme	novel	176:180	arg1	bacterium					182:190	a novel bacterium	174:190	a novel bacterium	174:190	A polyphasic taxonomic approach was used to characterize a novel bacterium, designated as strain HDW20T, isolated from the intestine of the dark diving beetle Hydrophilus acuminatus.
33289624	4	69	theme	average	761:767	arg1	identity					780:787	average nucleotide identity	761:787	a highest average nucleotide identity (ANI) value of 80.6 %	751:809	Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus Tessaracoccus in the phylum Actinobacteria and was closely related to T. flavescens SST-39T, T. defluvii JCM 17540T, and T. aquimaris NSG39T, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %.
33289624	4	69	theme	average	761:767	arg1	ANI					790:792	ANI	790:792	ANI	790:792	Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus Tessaracoccus in the phylum Actinobacteria and was closely related to T. flavescens SST-39T, T. defluvii JCM 17540T, and T. aquimaris NSG39T, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %.
33289624	9	70	theme	ʟʟ-diaminopimelic	1073:1089	arg1	components					1191:1200	sugar components	1185:1200	sugar components of the cell wall peptidoglycan	1185:1231	The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan.
33289624	9	70	theme	ʟʟ-diaminopimelic	1073:1089	arg1	ʟ-alanine					1097:1105	ʟ-alanine	1097:1105	ʟ-alanine	1097:1105	The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan.
33289624	9	70	theme	ʟʟ-diaminopimelic	1073:1089	arg1	ʟ-lysine					1112:1119	ʟ-lysine	1112:1119	ʟ-lysine	1112:1119	The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan.
33289624	9	70	theme	ʟʟ-diaminopimelic	1073:1089	arg1	acid					1091:1094	ʟʟ-diaminopimelic acid	1073:1094	ʟʟ-diaminopimelic acid	1073:1094	The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan.
33289624	9	70	theme	ʟʟ-diaminopimelic	1073:1089	arg1	components					1135:1144	amino acid components	1124:1144	amino acid components	1124:1144	The isolate contains ʟʟ-diaminopimelic acid, ʟ-alanine, and ʟ-lysine as amino acid components, and ribose, glucose, and galactose as sugar components of the cell wall peptidoglycan.
33289624	5	71	theme	cellular	822:829	arg1	acids					837:841	The major cellular fatty acids	812:841	The major cellular fatty acids	812:841	The major cellular fatty acids were C18 : 1 ω9c and anteiso-C15 : 0.
33289624	5	71	theme	cellular	822:829	arg1	C18 					848:851	C18 	848:851	C18 : 1 ω9c and anteiso-C15 : 0	848:878	The major cellular fatty acids were C18 : 1 ω9c and anteiso-C15 : 0.
33289624	2	72	attach	isolated	222:229	arg2	bacterium					182:190	a novel bacterium	174:190	a novel bacterium	174:190	A polyphasic taxonomic approach was used to characterize a novel bacterium, designated as strain HDW20T, isolated from the intestine of the dark diving beetle Hydrophilus acuminatus.
33289624	2	72	attach	isolated	222:229	arg1	intestine					240:248	the intestine	236:248	the intestine of the dark diving beetle	236:274	A polyphasic taxonomic approach was used to characterize a novel bacterium, designated as strain HDW20T, isolated from the intestine of the dark diving beetle Hydrophilus acuminatus.
33289624	4	73	theme	highest	753:759	arg1	value					795:799	a highest average nucleotide identity (ANI) value	751:799	a highest average nucleotide identity (ANI) value of 80.6 %	751:809	Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences showed that the isolate belonged to the genus Tessaracoccus in the phylum Actinobacteria and was closely related to T. flavescens SST-39T, T. defluvii JCM 17540T, and T. aquimaris NSG39T, with the highest 16S rRNA gene sequence similarity of 98.5 % and a highest average nucleotide identity (ANI) value of 80.6 %.
33289624	5	74	theme	fatty	831:835	arg1	acids					837:841	The major cellular fatty acids	812:841	The major cellular fatty acids	812:841	The major cellular fatty acids were C18 : 1 ω9c and anteiso-C15 : 0.
33289624	5	74	theme	fatty	831:835	arg1	C18 					848:851	C18 	848:851	C18 : 1 ω9c and anteiso-C15 : 0	848:878	The major cellular fatty acids were C18 : 1 ω9c and anteiso-C15 : 0.
33289624	11	75	theme	Tessaracoccus	1422:1434	arg1	sp					1450:1451	the name Tessaracoccus coleopterorum sp	1413:1451	the name Tessaracoccus coleopterorum sp	1413:1451	We propose the name Tessaracoccus coleopterorum sp.
32467865	7	0	theme	caloric	1288:1294	arg1	efficiency					1296:1305	lower food and caloric efficiency	1273:1305	efficiency	1296:1305	RESULTS Despite the tendency of higher food and caloric intake than the HFD (P = 0.04) group, the HFD + D group mice did not exhibit higher body weight, indicating lower food and caloric efficiency (P < 0.001).
32467865	4	1	theme	60	827:828	arg1	%					829:829	%	829:829	%	829:829	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	8	2	theme	fecal	1428:1432	arg1	P < 0.001					1451:1459	P < 0.001	1451:1459	P < 0.001	1451:1459	Sea vegetable supplementation reduced plasma monocyte chemotactic protein (MCP-1) (P < 0.001) and increased fecal lipid excretion (P < 0.001).
32467865	8	2	theme	fecal	1428:1432	arg1	excretion					1440:1448	increased fecal lipid excretion	1418:1448	increased fecal lipid excretion (P < 0.001)	1418:1460	Sea vegetable supplementation reduced plasma monocyte chemotactic protein (MCP-1) (P < 0.001) and increased fecal lipid excretion (P < 0.001).
32467865	4	3	dep	wt	888:889	arg1	wt					891:892	wt	891:892	wt	891:892	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	3	4	theme	Palmaria	542:549	arg1	mollis					551:556	Palmaria mollis	542:556	Palmaria mollis	542:556	OBJECTIVE We tested the hypothesis that dietary supplementation with either Pacific dulse (Palmaria mollis, red algae) or wakame (Undaria pinnatifida, brown algae) could remediate metabolic complications in high-fat diet-induced obesity.
32467865	3	4	theme	Palmaria	542:549	arg1	algae					563:567	red algae	559:567	red algae	559:567	OBJECTIVE We tested the hypothesis that dietary supplementation with either Pacific dulse (Palmaria mollis, red algae) or wakame (Undaria pinnatifida, brown algae) could remediate metabolic complications in high-fat diet-induced obesity.
32467865	4	5	theme	 kcal	830:834	arg1	fat					836:838	60% kcal fat	827:838	60% kcal fat	827:838	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	4	5	theme	 kcal	830:834	arg1	diet					815:818	high-fat diet	806:818	high-fat diet (HFD)	806:824	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	10	6	theme	metabolic	1889:1897	arg1	complications					1899:1911	obesity-associated metabolic complications	1870:1911	obesity-associated metabolic complications in C57BL/6J male mice	1870:1933	CONCLUSION Sea vegetable supplementation showed protective effects against obesity-associated metabolic complications in C57BL/6J male mice by increasing lipid excretion, reducing systemic inflammatory marker, and mitigating gut microbiome alteration.
32467865	4	7	dep	%	885:885	arg1	wakame					895:900	wakame (HFD + W)	895:910	HFD + 5% (wt:wt) wakame (HFD + W)	878:910	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	9	8	theme	Gut	1463:1465	arg1	analysis					1478:1485	Gut microbiome analysis	1463:1485	Gut microbiome analysis	1463:1485	Gut microbiome analysis showed that the HFD + D group had higher alpha-diversity than the HFD or LFD group, whereas beta-diversity analyses indicated that sea vegetable-supplemented HFD-fed mice (HFD + D and HFD + W groups) developed microbiome compositions more similar to those of the LFD-fed mice than those of the HFD-fed mice.
32467865	6	9	theme	fecal	1011:1015	arg1	lipids					1017:1022	fecal lipids	1011:1022	fecal lipids	1011:1022	Glucose tolerance, hepatic lipids, fecal lipids, and plasma markers were evaluated, and the gut microbiome composition was assessed.
32467865	11	10	theme	metabolic	2104:2112	arg1	issues					2114:2119	metabolic issues	2104:2119	metabolic issues related to lipid absorption, inflammation, and gut microbial balance	2104:2188	While the obese phenotype development was not prevented, metabolic issues related to lipid absorption, inflammation, and gut microbial balance were improved, showing therapeutic promise and warranting eventual mechanistic elucidations.
32467865	1	11	theme	nutrients	181:189	arg1	vegetables					150:159	BACKGROUND Sea vegetables	135:159	BACKGROUND Sea vegetables	135:159	BACKGROUND Sea vegetables are rich sources of nutrients as well as bioactive components that are linked to metabolic health improvement.
32467865	1	11	theme	nutrients	181:189	arg1	sources					170:176	rich sources	165:176	rich sources of nutrients as well as bioactive components that are linked to metabolic health improvement	165:269	BACKGROUND Sea vegetables are rich sources of nutrients as well as bioactive components that are linked to metabolic health improvement.
32467865	7	12	theme	group	1215:1219	arg1	mice					1221:1224	the HFD + D group mice	1203:1224	the HFD + D group mice	1203:1224	RESULTS Despite the tendency of higher food and caloric intake than the HFD (P = 0.04) group, the HFD + D group mice did not exhibit higher body weight, indicating lower food and caloric efficiency (P < 0.001).
32467865	10	13	theme	male	1925:1928	arg1	mice					1930:1933	C57BL/6J male mice	1916:1933	C57BL/6J male mice	1916:1933	CONCLUSION Sea vegetable supplementation showed protective effects against obesity-associated metabolic complications in C57BL/6J male mice by increasing lipid excretion, reducing systemic inflammatory marker, and mitigating gut microbiome alteration.
32467865	4	14	theme	caged	710:714	arg1	n = 8					731:735	n = 8	731:735	n = 8	731:735	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	4	14	theme	caged	710:714	arg1	mice					725:728	Individually caged C57BL/6J mice	697:728	METHODS Individually caged C57BL/6J mice (n = 8)	689:736	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	6	15	theme	gut	1068:1070	arg1	composition					1083:1093	the gut microbiome composition	1064:1093	the gut microbiome composition	1064:1093	Glucose tolerance, hepatic lipids, fecal lipids, and plasma markers were evaluated, and the gut microbiome composition was assessed.
32467865	4	16	with	%	885:885	arg1	diet					780:783	a low-fat diet	770:783	a low-fat diet (LFD)	770:789	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	4	16	with	%	885:885	arg1	LFD					786:788	LFD	786:788	LFD	786:788	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	9	17	theme	similar	1726:1732	arg1	compositions					1708:1719	microbiome compositions	1697:1719	microbiome compositions more similar to those of the LFD-fed mice	1697:1761	Gut microbiome analysis showed that the HFD + D group had higher alpha-diversity than the HFD or LFD group, whereas beta-diversity analyses indicated that sea vegetable-supplemented HFD-fed mice (HFD + D and HFD + W groups) developed microbiome compositions more similar to those of the LFD-fed mice than those of the HFD-fed mice.
32467865	10	18	theme	inflammatory	1984:1995	arg1	marker					1997:2002	systemic inflammatory marker	1975:2002	systemic inflammatory marker	1975:2002	CONCLUSION Sea vegetable supplementation showed protective effects against obesity-associated metabolic complications in C57BL/6J male mice by increasing lipid excretion, reducing systemic inflammatory marker, and mitigating gut microbiome alteration.
32467865	9	19	contain	had	1517:1519	arg1	group					1511:1515	the HFD + D group	1499:1515	the HFD + D group	1499:1515	Gut microbiome analysis showed that the HFD + D group had higher alpha-diversity than the HFD or LFD group, whereas beta-diversity analyses indicated that sea vegetable-supplemented HFD-fed mice (HFD + D and HFD + W groups) developed microbiome compositions more similar to those of the LFD-fed mice than those of the HFD-fed mice.
32467865	9	19	contain	had	1517:1519	arg2	alpha-diversity					1528:1542	higher alpha-diversity	1521:1542	higher alpha-diversity	1521:1542	Gut microbiome analysis showed that the HFD + D group had higher alpha-diversity than the HFD or LFD group, whereas beta-diversity analyses indicated that sea vegetable-supplemented HFD-fed mice (HFD + D and HFD + W groups) developed microbiome compositions more similar to those of the LFD-fed mice than those of the HFD-fed mice.
32467865	1	20	theme	bioactive	202:210	arg1	components					212:221	bioactive components	202:221	nutrients as well as bioactive components	181:221	BACKGROUND Sea vegetables are rich sources of nutrients as well as bioactive components that are linked to metabolic health improvement.
32467865	9	21	theme	HFD + D	1503:1509	arg1	group					1511:1515	the HFD + D group	1499:1515	the HFD + D group	1499:1515	Gut microbiome analysis showed that the HFD + D group had higher alpha-diversity than the HFD or LFD group, whereas beta-diversity analyses indicated that sea vegetable-supplemented HFD-fed mice (HFD + D and HFD + W groups) developed microbiome compositions more similar to those of the LFD-fed mice than those of the HFD-fed mice.
32467865	4	22	dep	diet	780:783	arg1	either					763:768	either	763:768	either	763:768	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	4	22	dep	diet	780:783	arg1	fat					801:803	10% kcal fat	792:803	10% kcal fat	792:803	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	10	23	theme	lipid	1949:1953	arg1	excretion					1955:1963	lipid excretion	1949:1963	lipid excretion	1949:1963	CONCLUSION Sea vegetable supplementation showed protective effects against obesity-associated metabolic complications in C57BL/6J male mice by increasing lipid excretion, reducing systemic inflammatory marker, and mitigating gut microbiome alteration.
32467865	3	24	with	supplementation	499:513	arg1	dulse					535:539	Pacific dulse	527:539	Pacific dulse (Palmaria mollis, red algae)	527:568	OBJECTIVE We tested the hypothesis that dietary supplementation with either Pacific dulse (Palmaria mollis, red algae) or wakame (Undaria pinnatifida, brown algae) could remediate metabolic complications in high-fat diet-induced obesity.
32467865	3	24	with	supplementation	499:513	arg1	wakame					573:578	wakame	573:578	wakame (Undaria pinnatifida, brown algae)	573:613	OBJECTIVE We tested the hypothesis that dietary supplementation with either Pacific dulse (Palmaria mollis, red algae) or wakame (Undaria pinnatifida, brown algae) could remediate metabolic complications in high-fat diet-induced obesity.
32467865	5	25	theme	Food	925:928	arg1	intake					930:935	Food intake	925:935	Food intake	925:935	Food intake and weight gain were monitored weekly.
32467865	7	26	theme	P = 0.04	1186:1193	arg1	group					1196:1200	the HFD (P = 0.04) group	1177:1200	the HFD (P = 0.04) group	1177:1200	RESULTS Despite the tendency of higher food and caloric intake than the HFD (P = 0.04) group, the HFD + D group mice did not exhibit higher body weight, indicating lower food and caloric efficiency (P < 0.001).
32467865	3	27	theme	brown	602:606	arg1	algae					608:612	brown algae	602:612	brown algae	602:612	OBJECTIVE We tested the hypothesis that dietary supplementation with either Pacific dulse (Palmaria mollis, red algae) or wakame (Undaria pinnatifida, brown algae) could remediate metabolic complications in high-fat diet-induced obesity.
32467865	3	27	theme	brown	602:606	arg1	pinnatifida					589:599	pinnatifida	589:599	pinnatifida	589:599	OBJECTIVE We tested the hypothesis that dietary supplementation with either Pacific dulse (Palmaria mollis, red algae) or wakame (Undaria pinnatifida, brown algae) could remediate metabolic complications in high-fat diet-induced obesity.
32467865	2	28	theme	Algal	272:276	arg1	polysaccharides					278:292	Algal polysaccharides	272:292	Algal polysaccharides	272:292	Algal polysaccharides improve satiety and modulate gut microbiota while proteins, peptides, and phenolic fractions exert anti-inflammatory, antioxidant, and antidiabetic effects.
32467865	7	29	theme	HFD	1181:1183	arg1	group					1196:1200	the HFD (P = 0.04) group	1177:1200	the HFD (P = 0.04) group	1177:1200	RESULTS Despite the tendency of higher food and caloric intake than the HFD (P = 0.04) group, the HFD + D group mice did not exhibit higher body weight, indicating lower food and caloric efficiency (P < 0.001).
32467865	10	30	theme	systemic	1975:1982	arg1	marker					1997:2002	systemic inflammatory marker	1975:2002	systemic inflammatory marker	1975:2002	CONCLUSION Sea vegetable supplementation showed protective effects against obesity-associated metabolic complications in C57BL/6J male mice by increasing lipid excretion, reducing systemic inflammatory marker, and mitigating gut microbiome alteration.
32467865	4	31	theme	ad	747:748	arg1	libitum					750:756	ad libitum	747:756	ad libitum with either a low-fat diet (LFD), 10% kcal fat	747:803	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	10	32	theme	CONCLUSION	1795:1804	arg1	supplementation					1820:1834	CONCLUSION Sea vegetable supplementation	1795:1834	CONCLUSION Sea vegetable supplementation	1795:1834	CONCLUSION Sea vegetable supplementation showed protective effects against obesity-associated metabolic complications in C57BL/6J male mice by increasing lipid excretion, reducing systemic inflammatory marker, and mitigating gut microbiome alteration.
32467865	11	33	theme	mechanistic	2257:2267	arg1	elucidations					2269:2280	eventual mechanistic elucidations	2248:2280	eventual mechanistic elucidations	2248:2280	While the obese phenotype development was not prevented, metabolic issues related to lipid absorption, inflammation, and gut microbial balance were improved, showing therapeutic promise and warranting eventual mechanistic elucidations.
32467865	1	34	theme	Sea	146:148	arg1	vegetables					150:159	BACKGROUND Sea vegetables	135:159	BACKGROUND Sea vegetables	135:159	BACKGROUND Sea vegetables are rich sources of nutrients as well as bioactive components that are linked to metabolic health improvement.
32467865	1	34	theme	Sea	146:148	arg1	sources					170:176	rich sources	165:176	rich sources of nutrients as well as bioactive components that are linked to metabolic health improvement	165:269	BACKGROUND Sea vegetables are rich sources of nutrients as well as bioactive components that are linked to metabolic health improvement.
32467865	11	35	theme	gut	2168:2170	arg1	balance					2182:2188	gut microbial balance	2168:2188	gut microbial balance	2168:2188	While the obese phenotype development was not prevented, metabolic issues related to lipid absorption, inflammation, and gut microbial balance were improved, showing therapeutic promise and warranting eventual mechanistic elucidations.
32467865	6	36	theme	plasma	1029:1034	arg1	markers					1036:1042	plasma markers	1029:1042	plasma markers	1029:1042	Glucose tolerance, hepatic lipids, fecal lipids, and plasma markers were evaluated, and the gut microbiome composition was assessed.
32467865	10	37	theme	gut	2020:2022	arg1	alteration					2035:2044	gut microbiome alteration	2020:2044	gut microbiome alteration	2020:2044	CONCLUSION Sea vegetable supplementation showed protective effects against obesity-associated metabolic complications in C57BL/6J male mice by increasing lipid excretion, reducing systemic inflammatory marker, and mitigating gut microbiome alteration.
32467865	0	38	theme	Metabolic	83:91	arg1	Benefits					93:100	Metabolic Benefits	83:100	Metabolic Benefits	83:100	Supplementation with Sea Vegetables Palmaria mollis and Undaria pinnatifida Exerts Metabolic Benefits in Diet-Induced Obesity in Mice.
32467865	10	39	theme	vegetable	1810:1818	arg1	supplementation					1820:1834	CONCLUSION Sea vegetable supplementation	1795:1834	CONCLUSION Sea vegetable supplementation	1795:1834	CONCLUSION Sea vegetable supplementation showed protective effects against obesity-associated metabolic complications in C57BL/6J male mice by increasing lipid excretion, reducing systemic inflammatory marker, and mitigating gut microbiome alteration.
32467865	11	40	theme	obese	2057:2061	arg1	phenotype					2063:2071	the obese phenotype	2053:2071	the obese phenotype development	2053:2083	While the obese phenotype development was not prevented, metabolic issues related to lipid absorption, inflammation, and gut microbial balance were improved, showing therapeutic promise and warranting eventual mechanistic elucidations.
32467865	3	41	dep	dulse	535:539	arg1	mollis					551:556	Palmaria mollis	542:556	Palmaria mollis	542:556	OBJECTIVE We tested the hypothesis that dietary supplementation with either Pacific dulse (Palmaria mollis, red algae) or wakame (Undaria pinnatifida, brown algae) could remediate metabolic complications in high-fat diet-induced obesity.
32467865	3	41	dep	dulse	535:539	arg1	algae					563:567	red algae	559:567	red algae	559:567	OBJECTIVE We tested the hypothesis that dietary supplementation with either Pacific dulse (Palmaria mollis, red algae) or wakame (Undaria pinnatifida, brown algae) could remediate metabolic complications in high-fat diet-induced obesity.
32467865	7	42	theme	intake	1165:1170	arg1	tendency					1129:1136	the tendency	1125:1136	the tendency of higher food and caloric intake than the HFD (P = 0.04) group	1125:1200	RESULTS Despite the tendency of higher food and caloric intake than the HFD (P = 0.04) group, the HFD + D group mice did not exhibit higher body weight, indicating lower food and caloric efficiency (P < 0.001).
32467865	8	43	theme	vegetable	1324:1332	arg1	supplementation					1334:1348	Sea vegetable supplementation	1320:1348	Sea vegetable supplementation	1320:1348	Sea vegetable supplementation reduced plasma monocyte chemotactic protein (MCP-1) (P < 0.001) and increased fecal lipid excretion (P < 0.001).
32467865	10	44	from	complications	1899:1911	arg1	mice					1930:1933	C57BL/6J male mice	1916:1933	C57BL/6J male mice	1916:1933	CONCLUSION Sea vegetable supplementation showed protective effects against obesity-associated metabolic complications in C57BL/6J male mice by increasing lipid excretion, reducing systemic inflammatory marker, and mitigating gut microbiome alteration.
32467865	4	45	with	%	848:848	arg1	diet					780:783	a low-fat diet	770:783	a low-fat diet (LFD)	770:789	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	4	45	with	%	848:848	arg1	LFD					786:788	LFD	786:788	LFD	786:788	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	3	46	theme	dietary	491:497	arg1	supplementation					499:513	dietary supplementation	491:513	dietary supplementation with either Pacific dulse (Palmaria mollis, red algae) or wakame (Undaria pinnatifida, brown algae)	491:613	OBJECTIVE We tested the hypothesis that dietary supplementation with either Pacific dulse (Palmaria mollis, red algae) or wakame (Undaria pinnatifida, brown algae) could remediate metabolic complications in high-fat diet-induced obesity.
32467865	0	47	theme	Sea	21:23	arg1	mollis					45:50	Palmaria mollis	36:50	Palmaria mollis	36:50	Supplementation with Sea Vegetables Palmaria mollis and Undaria pinnatifida Exerts Metabolic Benefits in Diet-Induced Obesity in Mice.
32467865	0	47	theme	Sea	21:23	arg1	pinnatifida					64:74	Undaria pinnatifida	56:74	Undaria pinnatifida	56:74	Supplementation with Sea Vegetables Palmaria mollis and Undaria pinnatifida Exerts Metabolic Benefits in Diet-Induced Obesity in Mice.
32467865	0	47	theme	Sea	21:23	arg1	Vegetables					25:34	Sea Vegetables Palmaria mollis and Undaria pinnatifida	21:74	Sea Vegetables Palmaria mollis and Undaria pinnatifida	21:74	Supplementation with Sea Vegetables Palmaria mollis and Undaria pinnatifida Exerts Metabolic Benefits in Diet-Induced Obesity in Mice.
32467865	11	48	theme	eventual	2248:2255	arg1	elucidations					2269:2280	eventual mechanistic elucidations	2248:2280	eventual mechanistic elucidations	2248:2280	While the obese phenotype development was not prevented, metabolic issues related to lipid absorption, inflammation, and gut microbial balance were improved, showing therapeutic promise and warranting eventual mechanistic elucidations.
32467865	7	49	theme	higher	1141:1146	arg1	food					1148:1151	higher food	1141:1151	higher food	1141:1151	RESULTS Despite the tendency of higher food and caloric intake than the HFD (P = 0.04) group, the HFD + D group mice did not exhibit higher body weight, indicating lower food and caloric efficiency (P < 0.001).
32467865	9	50	theme	vegetable-supplemented	1622:1643	arg1	mice					1653:1656	sea vegetable-supplemented HFD-fed mice	1618:1656	sea vegetable-supplemented HFD-fed mice (HFD + D and HFD + W groups)	1618:1685	Gut microbiome analysis showed that the HFD + D group had higher alpha-diversity than the HFD or LFD group, whereas beta-diversity analyses indicated that sea vegetable-supplemented HFD-fed mice (HFD + D and HFD + W groups) developed microbiome compositions more similar to those of the LFD-fed mice than those of the HFD-fed mice.
32467865	8	51	theme	monocyte	1365:1372	arg1	MCP-1					1395:1399	MCP-1	1395:1399	MCP-1	1395:1399	Sea vegetable supplementation reduced plasma monocyte chemotactic protein (MCP-1) (P < 0.001) and increased fecal lipid excretion (P < 0.001).
32467865	8	51	theme	monocyte	1365:1372	arg1	P < 0.001					1403:1411	P < 0.001	1403:1411	P < 0.001	1403:1411	Sea vegetable supplementation reduced plasma monocyte chemotactic protein (MCP-1) (P < 0.001) and increased fecal lipid excretion (P < 0.001).
32467865	8	51	theme	monocyte	1365:1372	arg1	protein					1386:1392	plasma monocyte chemotactic protein	1358:1392	plasma monocyte chemotactic protein (MCP-1) (P < 0.001)	1358:1412	Sea vegetable supplementation reduced plasma monocyte chemotactic protein (MCP-1) (P < 0.001) and increased fecal lipid excretion (P < 0.001).
32467865	2	52	theme	antidiabetic	429:440	arg1	effects					442:448	anti-inflammatory, antioxidant, and antidiabetic effects	393:448	anti-inflammatory, antioxidant, and antidiabetic effects	393:448	Algal polysaccharides improve satiety and modulate gut microbiota while proteins, peptides, and phenolic fractions exert anti-inflammatory, antioxidant, and antidiabetic effects.
32467865	0	53	theme	Palmaria	36:43	arg1	mollis					45:50	Palmaria mollis	36:50	Palmaria mollis	36:50	Supplementation with Sea Vegetables Palmaria mollis and Undaria pinnatifida Exerts Metabolic Benefits in Diet-Induced Obesity in Mice.
32467865	0	53	theme	Palmaria	36:43	arg1	Vegetables					25:34	Sea Vegetables Palmaria mollis and Undaria pinnatifida	21:74	Sea Vegetables Palmaria mollis and Undaria pinnatifida	21:74	Supplementation with Sea Vegetables Palmaria mollis and Undaria pinnatifida Exerts Metabolic Benefits in Diet-Induced Obesity in Mice.
32467865	9	54	dep	showed	1487:1492	arg1	whereas					1571:1577	whereas	1571:1577	whereas	1571:1577	Gut microbiome analysis showed that the HFD + D group had higher alpha-diversity than the HFD or LFD group, whereas beta-diversity analyses indicated that sea vegetable-supplemented HFD-fed mice (HFD + D and HFD + W groups) developed microbiome compositions more similar to those of the LFD-fed mice than those of the HFD-fed mice.
32467865	3	55	theme	high-fat	658:665	arg1	obesity					680:686	high-fat diet-induced obesity	658:686	high-fat diet-induced obesity	658:686	OBJECTIVE We tested the hypothesis that dietary supplementation with either Pacific dulse (Palmaria mollis, red algae) or wakame (Undaria pinnatifida, brown algae) could remediate metabolic complications in high-fat diet-induced obesity.
32467865	4	56	theme	high-fat	806:813	arg1	HFD					821:823	HFD	821:823	HFD	821:823	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	4	56	theme	high-fat	806:813	arg1	diet					815:818	high-fat diet	806:818	high-fat diet (HFD)	806:824	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	4	56	theme	high-fat	806:813	arg1	fat					836:838	60% kcal fat	827:838	60% kcal fat	827:838	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	3	57	theme	Pacific	527:533	arg1	dulse					535:539	Pacific dulse	527:539	Pacific dulse (Palmaria mollis, red algae)	527:568	OBJECTIVE We tested the hypothesis that dietary supplementation with either Pacific dulse (Palmaria mollis, red algae) or wakame (Undaria pinnatifida, brown algae) could remediate metabolic complications in high-fat diet-induced obesity.
32467865	10	58	theme	obesity-associated	1870:1887	arg1	complications					1899:1911	obesity-associated metabolic complications	1870:1911	obesity-associated metabolic complications in C57BL/6J male mice	1870:1933	CONCLUSION Sea vegetable supplementation showed protective effects against obesity-associated metabolic complications in C57BL/6J male mice by increasing lipid excretion, reducing systemic inflammatory marker, and mitigating gut microbiome alteration.
32467865	0	59	with	Supplementation	0:14	arg1	mollis					45:50	Palmaria mollis	36:50	Palmaria mollis	36:50	Supplementation with Sea Vegetables Palmaria mollis and Undaria pinnatifida Exerts Metabolic Benefits in Diet-Induced Obesity in Mice.
32467865	0	59	with	Supplementation	0:14	arg1	pinnatifida					64:74	Undaria pinnatifida	56:74	Undaria pinnatifida	56:74	Supplementation with Sea Vegetables Palmaria mollis and Undaria pinnatifida Exerts Metabolic Benefits in Diet-Induced Obesity in Mice.
32467865	0	59	with	Supplementation	0:14	arg1	Vegetables					25:34	Sea Vegetables Palmaria mollis and Undaria pinnatifida	21:74	Sea Vegetables Palmaria mollis and Undaria pinnatifida	21:74	Supplementation with Sea Vegetables Palmaria mollis and Undaria pinnatifida Exerts Metabolic Benefits in Diet-Induced Obesity in Mice.
32467865	0	60	from	Obesity	118:124	arg1	Mice					129:132	Mice	129:132	Mice	129:132	Supplementation with Sea Vegetables Palmaria mollis and Undaria pinnatifida Exerts Metabolic Benefits in Diet-Induced Obesity in Mice.
32467865	4	61	theme	%	794:794	arg1	fat					801:803	10% kcal fat	792:803	10% kcal fat	792:803	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	10	62	theme	C57BL/6J	1916:1923	arg1	mice					1930:1933	C57BL/6J male mice	1916:1933	C57BL/6J male mice	1916:1933	CONCLUSION Sea vegetable supplementation showed protective effects against obesity-associated metabolic complications in C57BL/6J male mice by increasing lipid excretion, reducing systemic inflammatory marker, and mitigating gut microbiome alteration.
32467865	2	63	theme	phenolic	368:375	arg1	fractions					377:385	phenolic fractions	368:385	phenolic fractions	368:385	Algal polysaccharides improve satiety and modulate gut microbiota while proteins, peptides, and phenolic fractions exert anti-inflammatory, antioxidant, and antidiabetic effects.
32467865	1	64	theme	health	252:257	arg1	improvement					259:269	metabolic health improvement	242:269	metabolic health improvement	242:269	BACKGROUND Sea vegetables are rich sources of nutrients as well as bioactive components that are linked to metabolic health improvement.
32467865	9	65	theme	W	1677:1677	arg1	groups					1679:1684	W groups	1677:1684	W groups	1677:1684	Gut microbiome analysis showed that the HFD + D group had higher alpha-diversity than the HFD or LFD group, whereas beta-diversity analyses indicated that sea vegetable-supplemented HFD-fed mice (HFD + D and HFD + W groups) developed microbiome compositions more similar to those of the LFD-fed mice than those of the HFD-fed mice.
32467865	8	66	theme	increased	1418:1426	arg1	P < 0.001					1451:1459	P < 0.001	1451:1459	P < 0.001	1451:1459	Sea vegetable supplementation reduced plasma monocyte chemotactic protein (MCP-1) (P < 0.001) and increased fecal lipid excretion (P < 0.001).
32467865	8	66	theme	increased	1418:1426	arg1	excretion					1440:1448	increased fecal lipid excretion	1418:1448	increased fecal lipid excretion (P < 0.001)	1418:1460	Sea vegetable supplementation reduced plasma monocyte chemotactic protein (MCP-1) (P < 0.001) and increased fecal lipid excretion (P < 0.001).
32467865	4	67	theme	%	829:829	arg1	fat					836:838	60% kcal fat	827:838	60% kcal fat	827:838	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	4	67	theme	%	829:829	arg1	diet					815:818	high-fat diet	806:818	high-fat diet (HFD)	806:824	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	7	68	theme	higher	1242:1247	arg1	weight					1254:1259	higher body weight	1242:1259	higher body weight	1242:1259	RESULTS Despite the tendency of higher food and caloric intake than the HFD (P = 0.04) group, the HFD + D group mice did not exhibit higher body weight, indicating lower food and caloric efficiency (P < 0.001).
32467865	8	69	theme	lipid	1434:1438	arg1	P < 0.001					1451:1459	P < 0.001	1451:1459	P < 0.001	1451:1459	Sea vegetable supplementation reduced plasma monocyte chemotactic protein (MCP-1) (P < 0.001) and increased fecal lipid excretion (P < 0.001).
32467865	8	69	theme	lipid	1434:1438	arg1	excretion					1440:1448	increased fecal lipid excretion	1418:1448	increased fecal lipid excretion (P < 0.001)	1418:1460	Sea vegetable supplementation reduced plasma monocyte chemotactic protein (MCP-1) (P < 0.001) and increased fecal lipid excretion (P < 0.001).
32467865	9	70	dep	mice	1653:1656	arg1	D					1665:1665	D	1665:1665	D	1665:1665	Gut microbiome analysis showed that the HFD + D group had higher alpha-diversity than the HFD or LFD group, whereas beta-diversity analyses indicated that sea vegetable-supplemented HFD-fed mice (HFD + D and HFD + W groups) developed microbiome compositions more similar to those of the LFD-fed mice than those of the HFD-fed mice.
32467865	9	70	dep	mice	1653:1656	arg1	HFD					1659:1661	HFD	1659:1661	HFD	1659:1661	Gut microbiome analysis showed that the HFD + D group had higher alpha-diversity than the HFD or LFD group, whereas beta-diversity analyses indicated that sea vegetable-supplemented HFD-fed mice (HFD + D and HFD + W groups) developed microbiome compositions more similar to those of the LFD-fed mice than those of the HFD-fed mice.
32467865	9	70	dep	mice	1653:1656	arg1	HFD					1671:1673	HFD	1671:1673	HFD	1671:1673	Gut microbiome analysis showed that the HFD + D group had higher alpha-diversity than the HFD or LFD group, whereas beta-diversity analyses indicated that sea vegetable-supplemented HFD-fed mice (HFD + D and HFD + W groups) developed microbiome compositions more similar to those of the LFD-fed mice than those of the HFD-fed mice.
32467865	3	71	theme	red	559:561	arg1	mollis					551:556	Palmaria mollis	542:556	Palmaria mollis	542:556	OBJECTIVE We tested the hypothesis that dietary supplementation with either Pacific dulse (Palmaria mollis, red algae) or wakame (Undaria pinnatifida, brown algae) could remediate metabolic complications in high-fat diet-induced obesity.
32467865	3	71	theme	red	559:561	arg1	algae					563:567	red algae	559:567	red algae	559:567	OBJECTIVE We tested the hypothesis that dietary supplementation with either Pacific dulse (Palmaria mollis, red algae) or wakame (Undaria pinnatifida, brown algae) could remediate metabolic complications in high-fat diet-induced obesity.
32467865	7	72	theme	body	1249:1252	arg1	weight					1254:1259	higher body weight	1242:1259	higher body weight	1242:1259	RESULTS Despite the tendency of higher food and caloric intake than the HFD (P = 0.04) group, the HFD + D group mice did not exhibit higher body weight, indicating lower food and caloric efficiency (P < 0.001).
32467865	9	73	theme	microbiome	1467:1476	arg1	analysis					1478:1485	Gut microbiome analysis	1463:1485	Gut microbiome analysis	1463:1485	Gut microbiome analysis showed that the HFD + D group had higher alpha-diversity than the HFD or LFD group, whereas beta-diversity analyses indicated that sea vegetable-supplemented HFD-fed mice (HFD + D and HFD + W groups) developed microbiome compositions more similar to those of the LFD-fed mice than those of the HFD-fed mice.
32467865	9	74	theme	microbiome	1697:1706	arg1	compositions					1708:1719	microbiome compositions	1697:1719	microbiome compositions more similar to those of the LFD-fed mice	1697:1761	Gut microbiome analysis showed that the HFD + D group had higher alpha-diversity than the HFD or LFD group, whereas beta-diversity analyses indicated that sea vegetable-supplemented HFD-fed mice (HFD + D and HFD + W groups) developed microbiome compositions more similar to those of the LFD-fed mice than those of the HFD-fed mice.
32467865	6	75	theme	microbiome	1072:1081	arg1	composition					1083:1093	the gut microbiome composition	1064:1093	the gut microbiome composition	1064:1093	Glucose tolerance, hepatic lipids, fecal lipids, and plasma markers were evaluated, and the gut microbiome composition was assessed.
32467865	6	76	theme	hepatic	995:1001	arg1	lipids					1003:1008	hepatic lipids	995:1008	hepatic lipids	995:1008	Glucose tolerance, hepatic lipids, fecal lipids, and plasma markers were evaluated, and the gut microbiome composition was assessed.
32467865	2	77	theme	gut	323:325	arg1	microbiota					327:336	gut microbiota	323:336	gut microbiota	323:336	Algal polysaccharides improve satiety and modulate gut microbiota while proteins, peptides, and phenolic fractions exert anti-inflammatory, antioxidant, and antidiabetic effects.
32467865	7	78	theme	HFD + D	1207:1213	arg1	mice					1221:1224	the HFD + D group mice	1203:1224	the HFD + D group mice	1203:1224	RESULTS Despite the tendency of higher food and caloric intake than the HFD (P = 0.04) group, the HFD + D group mice did not exhibit higher body weight, indicating lower food and caloric efficiency (P < 0.001).
32467865	1	79	attach	linked	232:237	arg2	sources					170:176	rich sources	165:176	rich sources of nutrients as well as bioactive components that are linked to metabolic health improvement	165:269	BACKGROUND Sea vegetables are rich sources of nutrients as well as bioactive components that are linked to metabolic health improvement.
32467865	1	79	attach	linked	232:237	arg1	improvement					259:269	metabolic health improvement	242:269	metabolic health improvement	242:269	BACKGROUND Sea vegetables are rich sources of nutrients as well as bioactive components that are linked to metabolic health improvement.
32467865	1	79	attach	linked	232:237	arg2	vegetables					150:159	BACKGROUND Sea vegetables	135:159	BACKGROUND Sea vegetables	135:159	BACKGROUND Sea vegetables are rich sources of nutrients as well as bioactive components that are linked to metabolic health improvement.
32467865	1	80	theme	components	212:221	arg1	vegetables					150:159	BACKGROUND Sea vegetables	135:159	BACKGROUND Sea vegetables	135:159	BACKGROUND Sea vegetables are rich sources of nutrients as well as bioactive components that are linked to metabolic health improvement.
32467865	1	80	theme	components	212:221	arg1	sources					170:176	rich sources	165:176	rich sources of nutrients as well as bioactive components that are linked to metabolic health improvement	165:269	BACKGROUND Sea vegetables are rich sources of nutrients as well as bioactive components that are linked to metabolic health improvement.
32467865	9	81	theme	higher	1521:1526	arg1	alpha-diversity					1528:1542	higher alpha-diversity	1521:1542	higher alpha-diversity	1521:1542	Gut microbiome analysis showed that the HFD + D group had higher alpha-diversity than the HFD or LFD group, whereas beta-diversity analyses indicated that sea vegetable-supplemented HFD-fed mice (HFD + D and HFD + W groups) developed microbiome compositions more similar to those of the LFD-fed mice than those of the HFD-fed mice.
32467865	9	82	theme	LFD-fed	1750:1756	arg1	mice					1758:1761	the LFD-fed mice	1746:1761	the LFD-fed mice	1746:1761	Gut microbiome analysis showed that the HFD + D group had higher alpha-diversity than the HFD or LFD group, whereas beta-diversity analyses indicated that sea vegetable-supplemented HFD-fed mice (HFD + D and HFD + W groups) developed microbiome compositions more similar to those of the LFD-fed mice than those of the HFD-fed mice.
32467865	4	83	with	libitum	750:756	arg1	diet					780:783	a low-fat diet	770:783	a low-fat diet (LFD)	770:789	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	4	83	with	libitum	750:756	arg1	LFD					786:788	LFD	786:788	LFD	786:788	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	3	84	theme	metabolic	631:639	arg1	complications					641:653	metabolic complications	631:653	metabolic complications	631:653	OBJECTIVE We tested the hypothesis that dietary supplementation with either Pacific dulse (Palmaria mollis, red algae) or wakame (Undaria pinnatifida, brown algae) could remediate metabolic complications in high-fat diet-induced obesity.
32467865	1	85	theme	BACKGROUND	135:144	arg1	vegetables					150:159	BACKGROUND Sea vegetables	135:159	BACKGROUND Sea vegetables	135:159	BACKGROUND Sea vegetables are rich sources of nutrients as well as bioactive components that are linked to metabolic health improvement.
32467865	1	85	theme	BACKGROUND	135:144	arg1	sources					170:176	rich sources	165:176	rich sources of nutrients as well as bioactive components that are linked to metabolic health improvement	165:269	BACKGROUND Sea vegetables are rich sources of nutrients as well as bioactive components that are linked to metabolic health improvement.
32467865	10	86	theme	microbiome	2024:2033	arg1	alteration					2035:2044	gut microbiome alteration	2020:2044	gut microbiome alteration	2020:2044	CONCLUSION Sea vegetable supplementation showed protective effects against obesity-associated metabolic complications in C57BL/6J male mice by increasing lipid excretion, reducing systemic inflammatory marker, and mitigating gut microbiome alteration.
32467865	11	87	theme	lipid	2132:2136	arg1	absorption					2138:2147	lipid absorption	2132:2147	lipid absorption	2132:2147	While the obese phenotype development was not prevented, metabolic issues related to lipid absorption, inflammation, and gut microbial balance were improved, showing therapeutic promise and warranting eventual mechanistic elucidations.
32467865	4	88	dep	%	848:848	arg1	dulse					858:862	dulse (HFD + D)	858:872	HFD + 5% (wt:wt) dulse (HFD + D)	841:872	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	3	89	dep	wakame	573:578	arg1	algae					608:612	brown algae	602:612	brown algae	602:612	OBJECTIVE We tested the hypothesis that dietary supplementation with either Pacific dulse (Palmaria mollis, red algae) or wakame (Undaria pinnatifida, brown algae) could remediate metabolic complications in high-fat diet-induced obesity.
32467865	3	89	dep	wakame	573:578	arg1	pinnatifida					589:599	pinnatifida	589:599	pinnatifida	589:599	OBJECTIVE We tested the hypothesis that dietary supplementation with either Pacific dulse (Palmaria mollis, red algae) or wakame (Undaria pinnatifida, brown algae) could remediate metabolic complications in high-fat diet-induced obesity.
32467865	9	90	theme	HFD	1553:1555	arg1	group					1564:1568	the HFD or LFD group	1549:1568	group	1564:1568	Gut microbiome analysis showed that the HFD + D group had higher alpha-diversity than the HFD or LFD group, whereas beta-diversity analyses indicated that sea vegetable-supplemented HFD-fed mice (HFD + D and HFD + W groups) developed microbiome compositions more similar to those of the LFD-fed mice than those of the HFD-fed mice.
32467865	4	91	theme	C57BL/6J	716:723	arg1	n = 8					731:735	n = 8	731:735	n = 8	731:735	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	4	91	theme	C57BL/6J	716:723	arg1	mice					725:728	Individually caged C57BL/6J mice	697:728	METHODS Individually caged C57BL/6J mice (n = 8)	689:736	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	1	92	theme	rich	165:168	arg1	vegetables					150:159	BACKGROUND Sea vegetables	135:159	BACKGROUND Sea vegetables	135:159	BACKGROUND Sea vegetables are rich sources of nutrients as well as bioactive components that are linked to metabolic health improvement.
32467865	1	92	theme	rich	165:168	arg1	sources					170:176	rich sources	165:176	rich sources of nutrients as well as bioactive components that are linked to metabolic health improvement	165:269	BACKGROUND Sea vegetables are rich sources of nutrients as well as bioactive components that are linked to metabolic health improvement.
32467865	9	93	theme	LFD	1560:1562	arg1	group					1564:1568	the HFD or LFD group	1549:1568	group	1564:1568	Gut microbiome analysis showed that the HFD + D group had higher alpha-diversity than the HFD or LFD group, whereas beta-diversity analyses indicated that sea vegetable-supplemented HFD-fed mice (HFD + D and HFD + W groups) developed microbiome compositions more similar to those of the LFD-fed mice than those of the HFD-fed mice.
32467865	9	94	theme	HFD-fed	1781:1787	arg1	mice					1789:1792	the HFD-fed mice	1777:1792	the HFD-fed mice	1777:1792	Gut microbiome analysis showed that the HFD + D group had higher alpha-diversity than the HFD or LFD group, whereas beta-diversity analyses indicated that sea vegetable-supplemented HFD-fed mice (HFD + D and HFD + W groups) developed microbiome compositions more similar to those of the LFD-fed mice than those of the HFD-fed mice.
32467865	0	95	theme	Diet-Induced	105:116	arg1	Obesity					118:124	Diet-Induced Obesity	105:124	Diet-Induced Obesity in Mice	105:132	Supplementation with Sea Vegetables Palmaria mollis and Undaria pinnatifida Exerts Metabolic Benefits in Diet-Induced Obesity in Mice.
32467865	4	96	with	diet	815:818	arg1	diet					780:783	a low-fat diet	770:783	a low-fat diet (LFD)	770:789	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	4	96	with	diet	815:818	arg1	LFD					786:788	LFD	786:788	LFD	786:788	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	8	97	theme	Sea	1320:1322	arg1	supplementation					1334:1348	Sea vegetable supplementation	1320:1348	Sea vegetable supplementation	1320:1348	Sea vegetable supplementation reduced plasma monocyte chemotactic protein (MCP-1) (P < 0.001) and increased fecal lipid excretion (P < 0.001).
32467865	4	98	theme	low-fat	772:778	arg1	diet					780:783	a low-fat diet	770:783	a low-fat diet (LFD)	770:789	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	4	98	theme	low-fat	772:778	arg1	LFD					786:788	LFD	786:788	LFD	786:788	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	10	99	theme	Sea	1806:1808	arg1	supplementation					1820:1834	CONCLUSION Sea vegetable supplementation	1795:1834	CONCLUSION Sea vegetable supplementation	1795:1834	CONCLUSION Sea vegetable supplementation showed protective effects against obesity-associated metabolic complications in C57BL/6J male mice by increasing lipid excretion, reducing systemic inflammatory marker, and mitigating gut microbiome alteration.
32467865	4	100	dep	wt	851:852	arg1	wt					854:855	wt	854:855	wt	854:855	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	5	101	theme	weight	941:946	arg1	gain					948:951	weight gain	941:951	weight gain	941:951	Food intake and weight gain were monitored weekly.
32467865	7	102	dep	RESULTS	1109:1115	arg1	exhibit					1234:1240	exhibit	1234:1240	exhibit higher body weight	1234:1259	RESULTS Despite the tendency of higher food and caloric intake than the HFD (P = 0.04) group, the HFD + D group mice did not exhibit higher body weight, indicating lower food and caloric efficiency (P < 0.001).
32467865	7	103	theme	caloric	1157:1163	arg1	intake					1165:1170	caloric intake	1157:1170	caloric intake	1157:1170	RESULTS Despite the tendency of higher food and caloric intake than the HFD (P = 0.04) group, the HFD + D group mice did not exhibit higher body weight, indicating lower food and caloric efficiency (P < 0.001).
32467865	9	104	theme	beta-diversity	1579:1592	arg1	analyses					1594:1601	beta-diversity analyses	1579:1601	beta-diversity analyses	1579:1601	Gut microbiome analysis showed that the HFD + D group had higher alpha-diversity than the HFD or LFD group, whereas beta-diversity analyses indicated that sea vegetable-supplemented HFD-fed mice (HFD + D and HFD + W groups) developed microbiome compositions more similar to those of the LFD-fed mice than those of the HFD-fed mice.
32467865	2	105	theme	antioxidant	412:422	arg1	effects					442:448	anti-inflammatory, antioxidant, and antidiabetic effects	393:448	anti-inflammatory, antioxidant, and antidiabetic effects	393:448	Algal polysaccharides improve satiety and modulate gut microbiota while proteins, peptides, and phenolic fractions exert anti-inflammatory, antioxidant, and antidiabetic effects.
32467865	7	106	theme	food	1148:1151	arg1	tendency					1129:1136	the tendency	1125:1136	the tendency of higher food and caloric intake than the HFD (P = 0.04) group	1125:1200	RESULTS Despite the tendency of higher food and caloric intake than the HFD (P = 0.04) group, the HFD + D group mice did not exhibit higher body weight, indicating lower food and caloric efficiency (P < 0.001).
32467865	8	107	theme	plasma	1358:1363	arg1	MCP-1					1395:1399	MCP-1	1395:1399	MCP-1	1395:1399	Sea vegetable supplementation reduced plasma monocyte chemotactic protein (MCP-1) (P < 0.001) and increased fecal lipid excretion (P < 0.001).
32467865	8	107	theme	plasma	1358:1363	arg1	P < 0.001					1403:1411	P < 0.001	1403:1411	P < 0.001	1403:1411	Sea vegetable supplementation reduced plasma monocyte chemotactic protein (MCP-1) (P < 0.001) and increased fecal lipid excretion (P < 0.001).
32467865	8	107	theme	plasma	1358:1363	arg1	protein					1386:1392	plasma monocyte chemotactic protein	1358:1392	plasma monocyte chemotactic protein (MCP-1) (P < 0.001)	1358:1412	Sea vegetable supplementation reduced plasma monocyte chemotactic protein (MCP-1) (P < 0.001) and increased fecal lipid excretion (P < 0.001).
32467865	11	108	theme	therapeutic	2213:2223	arg1	promise					2225:2231	therapeutic promise	2213:2231	therapeutic promise	2213:2231	While the obese phenotype development was not prevented, metabolic issues related to lipid absorption, inflammation, and gut microbial balance were improved, showing therapeutic promise and warranting eventual mechanistic elucidations.
32467865	2	109	theme	anti-inflammatory	393:409	arg1	effects					442:448	anti-inflammatory, antioxidant, and antidiabetic effects	393:448	anti-inflammatory, antioxidant, and antidiabetic effects	393:448	Algal polysaccharides improve satiety and modulate gut microbiota while proteins, peptides, and phenolic fractions exert anti-inflammatory, antioxidant, and antidiabetic effects.
32467865	7	110	dep	food	1279:1282	arg1	lower					1273:1277	lower	1273:1277	lower	1273:1277	RESULTS Despite the tendency of higher food and caloric intake than the HFD (P = 0.04) group, the HFD + D group mice did not exhibit higher body weight, indicating lower food and caloric efficiency (P < 0.001).
32467865	9	111	theme	sea	1618:1620	arg1	mice					1653:1656	sea vegetable-supplemented HFD-fed mice	1618:1656	sea vegetable-supplemented HFD-fed mice (HFD + D and HFD + W groups)	1618:1685	Gut microbiome analysis showed that the HFD + D group had higher alpha-diversity than the HFD or LFD group, whereas beta-diversity analyses indicated that sea vegetable-supplemented HFD-fed mice (HFD + D and HFD + W groups) developed microbiome compositions more similar to those of the LFD-fed mice than those of the HFD-fed mice.
32467865	8	112	theme	chemotactic	1374:1384	arg1	MCP-1					1395:1399	MCP-1	1395:1399	MCP-1	1395:1399	Sea vegetable supplementation reduced plasma monocyte chemotactic protein (MCP-1) (P < 0.001) and increased fecal lipid excretion (P < 0.001).
32467865	8	112	theme	chemotactic	1374:1384	arg1	P < 0.001					1403:1411	P < 0.001	1403:1411	P < 0.001	1403:1411	Sea vegetable supplementation reduced plasma monocyte chemotactic protein (MCP-1) (P < 0.001) and increased fecal lipid excretion (P < 0.001).
32467865	8	112	theme	chemotactic	1374:1384	arg1	protein					1386:1392	plasma monocyte chemotactic protein	1358:1392	plasma monocyte chemotactic protein (MCP-1) (P < 0.001)	1358:1412	Sea vegetable supplementation reduced plasma monocyte chemotactic protein (MCP-1) (P < 0.001) and increased fecal lipid excretion (P < 0.001).
32467865	3	113	theme	diet-induced	667:678	arg1	obesity					680:686	high-fat diet-induced obesity	658:686	high-fat diet-induced obesity	658:686	OBJECTIVE We tested the hypothesis that dietary supplementation with either Pacific dulse (Palmaria mollis, red algae) or wakame (Undaria pinnatifida, brown algae) could remediate metabolic complications in high-fat diet-induced obesity.
32467865	11	114	theme	related	2121:2127	arg1	issues					2114:2119	metabolic issues	2104:2119	metabolic issues related to lipid absorption, inflammation, and gut microbial balance	2104:2188	While the obese phenotype development was not prevented, metabolic issues related to lipid absorption, inflammation, and gut microbial balance were improved, showing therapeutic promise and warranting eventual mechanistic elucidations.
32467865	3	115	dep	OBJECTIVE	451:459	arg1	tested					464:469	tested	464:469	tested the hypothesis that dietary supplementation with either Pacific dulse (Palmaria mollis, red algae) or wakame (Undaria pinnatifida, brown algae) could remediate metabolic complications in high-fat diet-induced obesity	464:686	OBJECTIVE We tested the hypothesis that dietary supplementation with either Pacific dulse (Palmaria mollis, red algae) or wakame (Undaria pinnatifida, brown algae) could remediate metabolic complications in high-fat diet-induced obesity.
32467865	9	116	theme	HFD-fed	1645:1651	arg1	mice					1653:1656	sea vegetable-supplemented HFD-fed mice	1618:1656	sea vegetable-supplemented HFD-fed mice (HFD + D and HFD + W groups)	1618:1685	Gut microbiome analysis showed that the HFD + D group had higher alpha-diversity than the HFD or LFD group, whereas beta-diversity analyses indicated that sea vegetable-supplemented HFD-fed mice (HFD + D and HFD + W groups) developed microbiome compositions more similar to those of the LFD-fed mice than those of the HFD-fed mice.
32467865	4	117	theme	 kcal	795:799	arg1	fat					801:803	10% kcal fat	792:803	10% kcal fat	792:803	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	11	118	theme	phenotype	2063:2071	arg1	development					2073:2083	the obese phenotype development	2053:2083	the obese phenotype development	2053:2083	While the obese phenotype development was not prevented, metabolic issues related to lipid absorption, inflammation, and gut microbial balance were improved, showing therapeutic promise and warranting eventual mechanistic elucidations.
32467865	0	119	theme	Undaria	56:62	arg1	pinnatifida					64:74	Undaria pinnatifida	56:74	Undaria pinnatifida	56:74	Supplementation with Sea Vegetables Palmaria mollis and Undaria pinnatifida Exerts Metabolic Benefits in Diet-Induced Obesity in Mice.
32467865	0	119	theme	Undaria	56:62	arg1	Vegetables					25:34	Sea Vegetables Palmaria mollis and Undaria pinnatifida	21:74	Sea Vegetables Palmaria mollis and Undaria pinnatifida	21:74	Supplementation with Sea Vegetables Palmaria mollis and Undaria pinnatifida Exerts Metabolic Benefits in Diet-Induced Obesity in Mice.
32467865	6	120	theme	Glucose	976:982	arg1	tolerance					984:992	Glucose tolerance	976:992	Glucose tolerance	976:992	Glucose tolerance, hepatic lipids, fecal lipids, and plasma markers were evaluated, and the gut microbiome composition was assessed.
32467865	0	121	dep	Vegetables	25:34	arg1	mollis					45:50	Palmaria mollis	36:50	Palmaria mollis	36:50	Supplementation with Sea Vegetables Palmaria mollis and Undaria pinnatifida Exerts Metabolic Benefits in Diet-Induced Obesity in Mice.
32467865	0	121	dep	Vegetables	25:34	arg1	pinnatifida					64:74	Undaria pinnatifida	56:74	Undaria pinnatifida	56:74	Supplementation with Sea Vegetables Palmaria mollis and Undaria pinnatifida Exerts Metabolic Benefits in Diet-Induced Obesity in Mice.
32467865	0	121	dep	Vegetables	25:34	arg1	Vegetables					25:34	Sea Vegetables Palmaria mollis and Undaria pinnatifida	21:74	Sea Vegetables Palmaria mollis and Undaria pinnatifida	21:74	Supplementation with Sea Vegetables Palmaria mollis and Undaria pinnatifida Exerts Metabolic Benefits in Diet-Induced Obesity in Mice.
32467865	4	122	dep	METHODS	689:695	arg1	n = 8					731:735	n = 8	731:735	n = 8	731:735	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	4	122	dep	METHODS	689:695	arg1	mice					725:728	Individually caged C57BL/6J mice	697:728	METHODS Individually caged C57BL/6J mice (n = 8)	689:736	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	11	123	theme	microbial	2172:2180	arg1	balance					2182:2188	gut microbial balance	2168:2188	gut microbial balance	2168:2188	While the obese phenotype development was not prevented, metabolic issues related to lipid absorption, inflammation, and gut microbial balance were improved, showing therapeutic promise and warranting eventual mechanistic elucidations.
32467865	10	124	theme	protective	1843:1852	arg1	effects					1854:1860	protective effects	1843:1860	protective effects	1843:1860	CONCLUSION Sea vegetable supplementation showed protective effects against obesity-associated metabolic complications in C57BL/6J male mice by increasing lipid excretion, reducing systemic inflammatory marker, and mitigating gut microbiome alteration.
32467865	4	125	theme	10	792:793	arg1	%					794:794	%	794:794	%	794:794	METHODS Individually caged C57BL/6J mice (n = 8) were fed ad libitum with either a low-fat diet (LFD), 10% kcal fat; high-fat diet (HFD), 60% kcal fat; HFD + 5% (wt:wt) dulse (HFD + D); or HFD + 5% (wt:wt) wakame (HFD + W) for 8 weeks.
32467865	1	126	theme	metabolic	242:250	arg1	improvement					259:269	metabolic health improvement	242:269	metabolic health improvement	242:269	BACKGROUND Sea vegetables are rich sources of nutrients as well as bioactive components that are linked to metabolic health improvement.
33459818	2	0	dep	forming	189:195	arg1	non-spore					179:187	non-spore	179:187	non-spore	179:187	A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium, designated strain NCCP-1664T, was isolated from Cholistan desert, Pakistan.
33459818	2	1	theme	Gram-staining	155:167	arg1	bacterium					227:235	A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium	153:235	A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium	153:235	A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium, designated strain NCCP-1664T, was isolated from Cholistan desert, Pakistan.
33459818	8	2	dep	physiological	1717:1729	arg1	biochemical					1732:1742	biochemical	1732:1742	biochemical	1732:1742	The phylogenomic analyses along with chemotaxonomic data, physiological, biochemical characteristics allowed to describe it as representative of a novel genus, for which the name Zafaria cholistanensis gen. nov. sp.
33459818	7	3	theme	L-Lys-L	1254:1260	arg1	-Ala					1262:1265	A3α L-Lys-L -Ala	1250:1265	A3α L-Lys-L -Ala	1250:1265	Chemotaxonomic data of strain NCCP-1664T showed the peptidoglycan type as A3α L-Lys-L -Ala; menaquinones as MK-9(H2) (67%), MK-8(H2) (32%) and MK-7(H2) (1%), major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids.
33459818	4	4	theme	gene	593:596	arg1	sequence					598:605	16S rRNA gene sequence	584:605	16S rRNA gene sequence	584:605	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
33459818	7	5	theme	polar	1424:1428	arg1	lipids					1430:1435	polar lipids	1424:1435	polar lipids	1424:1435	Chemotaxonomic data of strain NCCP-1664T showed the peptidoglycan type as A3α L-Lys-L -Ala; menaquinones as MK-9(H2) (67%), MK-8(H2) (32%) and MK-7(H2) (1%), major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids.
33459818	3	6	theme	%	532:532	arg1	NaCl					534:537	0-16% NaCl	528:537	0-16% NaCl (optimum 2%)	528:550	Cells of strain NCCP-1664T were strictly aerobic, catalase positive and oxidase negative with a rod to coccus growth cycle and can grow at pH 6.0-9.0 (optimum pH 7-8) at 28-45 °C (optimum 37 °C) and could tolerate 0-16% NaCl (optimum 2%).
33459818	3	6	theme	%	532:532	arg1	%					549:549	optimum 2%	540:549	optimum 2%	540:549	Cells of strain NCCP-1664T were strictly aerobic, catalase positive and oxidase negative with a rod to coccus growth cycle and can grow at pH 6.0-9.0 (optimum pH 7-8) at 28-45 °C (optimum 37 °C) and could tolerate 0-16% NaCl (optimum 2%).
33459818	7	7	theme	NCCP-1664T	1206:1215	arg1	data					1191:1194	Chemotaxonomic data	1176:1194	Chemotaxonomic data of strain NCCP-1664T	1176:1215	Chemotaxonomic data of strain NCCP-1664T showed the peptidoglycan type as A3α L-Lys-L -Ala; menaquinones as MK-9(H2) (67%), MK-8(H2) (32%) and MK-7(H2) (1%), major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids.
33459818	7	8	theme	peptidoglycan	1228:1240	arg1	type					1242:1245	the peptidoglycan type	1224:1245	the peptidoglycan type	1224:1245	Chemotaxonomic data of strain NCCP-1664T showed the peptidoglycan type as A3α L-Lys-L -Ala; menaquinones as MK-9(H2) (67%), MK-8(H2) (32%) and MK-7(H2) (1%), major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids.
33459818	9	9	theme	strain	1906:1911	arg1	NCCP-1664T					1913:1922	the type strain NCCP-1664T	1897:1922	the type strain NCCP-1664T (= DSM 29936T = KCTC 39549T)	1897:1951	nov. is proposed with the type strain NCCP-1664T (= DSM 29936T = KCTC 39549T).
33459818	9	9	theme	strain	1906:1911	arg1	39549T					1945:1950	= DSM 29936T = KCTC 39549T	1925:1950	= DSM 29936T = KCTC 39549T	1925:1950	nov. is proposed with the type strain NCCP-1664T (= DSM 29936T = KCTC 39549T).
33459818	6	10	theme	70.0 mol	1165:1172	arg1	%					1173:1173	70.0 mol%	1165:1173	70.0 mol%	1165:1173	DNA G + C content of strain NCCP-1664T was 70.0 mol%.
33459818	5	11	theme	mentioned	949:957	arg1	strains					964:970	above mentioned type strains	943:970	above mentioned type strains	943:970	DNA-DNA relatedness values of strain NCCP-1664T with above mentioned type strains were found to be less than 54%, whereas digital DDH and average nucleotide identity (ANI) values with A. oryzae were 20.9 and of 74.3%, respectively.
33459818	7	12	theme	fatty	1340:1344	arg1	acids					1346:1350	major fatty acids	1334:1350	major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids	1334:1656	Chemotaxonomic data of strain NCCP-1664T showed the peptidoglycan type as A3α L-Lys-L -Ala; menaquinones as MK-9(H2) (67%), MK-8(H2) (32%) and MK-7(H2) (1%), major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids.
33459818	7	12	theme	fatty	1340:1344	arg1	MK-9					1284:1287	MK-9	1284:1287	MK-9(H2) (67%)	1284:1297	Chemotaxonomic data of strain NCCP-1664T showed the peptidoglycan type as A3α L-Lys-L -Ala; menaquinones as MK-9(H2) (67%), MK-8(H2) (32%) and MK-7(H2) (1%), major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids.
33459818	4	13	theme	16S	584:586	arg1	sequence					598:605	16S rRNA gene sequence	584:605	16S rRNA gene sequence	584:605	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
33459818	6	14	theme	NCCP-1664T	1150:1159	arg1	content					1132:1138	DNA G + C content	1122:1138	DNA G + C content of strain NCCP-1664T	1122:1159	DNA G + C content of strain NCCP-1664T was 70.0 mol%.
33459818	7	15	theme	strain	1199:1204	arg1	NCCP-1664T					1206:1215	strain NCCP-1664T	1199:1215	strain NCCP-1664T	1199:1215	Chemotaxonomic data of strain NCCP-1664T showed the peptidoglycan type as A3α L-Lys-L -Ala; menaquinones as MK-9(H2) (67%), MK-8(H2) (32%) and MK-7(H2) (1%), major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids.
33459818	9	16	theme	29936T = KCTC	1931:1943	arg1	NCCP-1664T					1913:1922	the type strain NCCP-1664T	1897:1922	the type strain NCCP-1664T (= DSM 29936T = KCTC 39549T)	1897:1951	nov. is proposed with the type strain NCCP-1664T (= DSM 29936T = KCTC 39549T).
33459818	9	16	theme	29936T = KCTC	1931:1943	arg1	39549T					1945:1950	= DSM 29936T = KCTC 39549T	1925:1950	= DSM 29936T = KCTC 39549T	1925:1950	nov. is proposed with the type strain NCCP-1664T (= DSM 29936T = KCTC 39549T).
33459818	8	17	theme	phylogenomic	1663:1674	arg1	analyses					1676:1683	The phylogenomic analyses	1659:1683	The phylogenomic analyses along with chemotaxonomic data, physiological, biochemical characteristics	1659:1758	The phylogenomic analyses along with chemotaxonomic data, physiological, biochemical characteristics allowed to describe it as representative of a novel genus, for which the name Zafaria cholistanensis gen. nov. sp.
33459818	5	18	theme	strain	920:925	arg1	NCCP-1664T					927:936	strain NCCP-1664T	920:936	strain NCCP-1664T	920:936	DNA-DNA relatedness values of strain NCCP-1664T with above mentioned type strains were found to be less than 54%, whereas digital DDH and average nucleotide identity (ANI) values with A. oryzae were 20.9 and of 74.3%, respectively.
33459818	1	19	theme	desert	128:133	arg1	soil					135:138	Cholistan desert soil	118:138	Cholistan desert soil of Pakistan	118:150	nov., a moderately thermotolerant and halotolerant actinobacterium isolated from Cholistan desert soil of Pakistan.
33459818	6	20	theme	G + C	1126:1130	arg1	content					1132:1138	DNA G + C content	1122:1138	DNA G + C content of strain NCCP-1664T	1122:1159	DNA G + C content of strain NCCP-1664T was 70.0 mol%.
33459818	2	21	attach	isolated	272:279	arg2	bacterium					227:235	A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium	153:235	A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium	153:235	A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium, designated strain NCCP-1664T, was isolated from Cholistan desert, Pakistan.
33459818	2	21	attach	isolated	272:279	arg1	Pakistan					304:311	Pakistan	304:311	Pakistan	304:311	A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium, designated strain NCCP-1664T, was isolated from Cholistan desert, Pakistan.
33459818	7	22	dep	-C15:0	1363:1368	arg1	profile					1437:1443	profile	1437:1443	profile	1437:1443	Chemotaxonomic data of strain NCCP-1664T showed the peptidoglycan type as A3α L-Lys-L -Ala; menaquinones as MK-9(H2) (67%), MK-8(H2) (32%) and MK-7(H2) (1%), major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids.
33459818	4	23	theme	strain	621:626	arg1	NCCP-1664T					628:637	strain NCCP-1664T	621:637	strain NCCP-1664T	621:637	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
33459818	8	24	theme	chemotaxonomic	1696:1709	arg1	characteristics					1744:1758	physiological, biochemical characteristics	1717:1758	physiological, biochemical characteristics	1717:1758	The phylogenomic analyses along with chemotaxonomic data, physiological, biochemical characteristics allowed to describe it as representative of a novel genus, for which the name Zafaria cholistanensis gen. nov. sp.
33459818	8	24	theme	chemotaxonomic	1696:1709	arg1	data					1711:1714	chemotaxonomic data	1696:1714	chemotaxonomic data	1696:1714	The phylogenomic analyses along with chemotaxonomic data, physiological, biochemical characteristics allowed to describe it as representative of a novel genus, for which the name Zafaria cholistanensis gen. nov. sp.
33459818	3	25	theme	strain	323:328	arg1	NCCP-1664T					330:339	strain NCCP-1664T	323:339	strain NCCP-1664T	323:339	Cells of strain NCCP-1664T were strictly aerobic, catalase positive and oxidase negative with a rod to coccus growth cycle and can grow at pH 6.0-9.0 (optimum pH 7-8) at 28-45 °C (optimum 37 °C) and could tolerate 0-16% NaCl (optimum 2%).
33459818	4	26	theme	genus	710:714	arg1	Arthrobacter					716:727	the genus Arthrobacter	706:727	the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa	706:887	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
33459818	8	27	theme	genus	1812:1816	arg1	representative					1786:1799	representative	1786:1799	representative of a novel genus, for which the name Zafaria cholistanensis gen. nov. sp	1786:1872	The phylogenomic analyses along with chemotaxonomic data, physiological, biochemical characteristics allowed to describe it as representative of a novel genus, for which the name Zafaria cholistanensis gen. nov. sp.
33459818	3	28	theme	growth	424:429	arg1	cycle					431:435	coccus growth cycle	417:435	coccus growth cycle	417:435	Cells of strain NCCP-1664T were strictly aerobic, catalase positive and oxidase negative with a rod to coccus growth cycle and can grow at pH 6.0-9.0 (optimum pH 7-8) at 28-45 °C (optimum 37 °C) and could tolerate 0-16% NaCl (optimum 2%).
33459818	0	29	theme	Zafaria	0:6	arg1	nov.					28:31	Zafaria cholistanensis gen. nov.	0:31	Zafaria cholistanensis gen. nov.	0:31	Zafaria cholistanensis gen. nov. sp.
33459818	5	30	theme	average	1028:1034	arg1	identity					1047:1054	average nucleotide identity	1028:1054	average nucleotide identity	1028:1054	DNA-DNA relatedness values of strain NCCP-1664T with above mentioned type strains were found to be less than 54%, whereas digital DDH and average nucleotide identity (ANI) values with A. oryzae were 20.9 and of 74.3%, respectively.
33459818	0	31	theme	gen.	23:26	arg1	nov.					28:31	Zafaria cholistanensis gen. nov.	0:31	Zafaria cholistanensis gen. nov.	0:31	Zafaria cholistanensis gen. nov. sp.
33459818	5	32	theme	identity	1047:1054	arg1	values					1062:1067	digital DDH and average nucleotide identity (ANI) values	1012:1067	digital DDH and average nucleotide identity (ANI) values with A. oryzae	1012:1082	DNA-DNA relatedness values of strain NCCP-1664T with above mentioned type strains were found to be less than 54%, whereas digital DDH and average nucleotide identity (ANI) values with A. oryzae were 20.9 and of 74.3%, respectively.
33459818	7	33	theme	anteiso	1355:1361	arg1	%					1417:1417	6.9%	1414:1417	6.9%	1414:1417	Chemotaxonomic data of strain NCCP-1664T showed the peptidoglycan type as A3α L-Lys-L -Ala; menaquinones as MK-9(H2) (67%), MK-8(H2) (32%) and MK-7(H2) (1%), major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids.
33459818	7	33	theme	anteiso	1355:1361	arg1	-C15:0					1363:1368	anteiso -C15:0	1355:1368	anteiso -C15:0 (51.2%)	1355:1376	Chemotaxonomic data of strain NCCP-1664T showed the peptidoglycan type as A3α L-Lys-L -Ala; menaquinones as MK-9(H2) (67%), MK-8(H2) (32%) and MK-7(H2) (1%), major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids.
33459818	7	33	theme	anteiso	1355:1361	arg1	%					1375:1375	51.2%	1371:1375	51.2%	1371:1375	Chemotaxonomic data of strain NCCP-1664T showed the peptidoglycan type as A3α L-Lys-L -Ala; menaquinones as MK-9(H2) (67%), MK-8(H2) (32%) and MK-7(H2) (1%), major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids.
33459818	4	34	theme	related	876:882	arg1	taxa					884:887	other related taxa	870:887	other related taxa	870:887	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
33459818	4	35	theme	other	870:874	arg1	taxa					884:887	other related taxa	870:887	other related taxa	870:887	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
33459818	5	36	theme	relatedness	898:908	arg1	values					910:915	DNA-DNA relatedness values	890:915	DNA-DNA relatedness values of strain NCCP-1664T with above mentioned type strains	890:970	DNA-DNA relatedness values of strain NCCP-1664T with above mentioned type strains were found to be less than 54%, whereas digital DDH and average nucleotide identity (ANI) values with A. oryzae were 20.9 and of 74.3%, respectively.
33459818	8	37	theme	Zafaria	1838:1844	arg1	nov.					1866:1869	name Zafaria cholistanensis gen. nov.	1833:1869	the name Zafaria cholistanensis gen. nov. sp	1829:1872	The phylogenomic analyses along with chemotaxonomic data, physiological, biochemical characteristics allowed to describe it as representative of a novel genus, for which the name Zafaria cholistanensis gen. nov. sp.
33459818	2	38	theme	positive	169:176	arg1	bacterium					227:235	A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium	153:235	A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium	153:235	A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium, designated strain NCCP-1664T, was isolated from Cholistan desert, Pakistan.
33459818	5	39	theme	DDH	1020:1022	arg1	values					1062:1067	digital DDH and average nucleotide identity (ANI) values	1012:1067	digital DDH and average nucleotide identity (ANI) values with A. oryzae	1012:1082	DNA-DNA relatedness values of strain NCCP-1664T with above mentioned type strains were found to be less than 54%, whereas digital DDH and average nucleotide identity (ANI) values with A. oryzae were 20.9 and of 74.3%, respectively.
33459818	7	40	theme	monogalactosyldiacylglycerol	1572:1599	arg1	phosphatidylglycerol					1483:1502	phosphatidylglycerol	1483:1502	phosphatidylglycerol	1483:1502	Chemotaxonomic data of strain NCCP-1664T showed the peptidoglycan type as A3α L-Lys-L -Ala; menaquinones as MK-9(H2) (67%), MK-8(H2) (32%) and MK-7(H2) (1%), major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids.
33459818	7	40	theme	monogalactosyldiacylglycerol	1572:1599	arg1	amounts					1561:1567	small amounts	1555:1567	small amounts of monogalactosyldiacylglycerol	1555:1599	Chemotaxonomic data of strain NCCP-1664T showed the peptidoglycan type as A3α L-Lys-L -Ala; menaquinones as MK-9(H2) (67%), MK-8(H2) (32%) and MK-7(H2) (1%), major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids.
33459818	7	40	theme	monogalactosyldiacylglycerol	1572:1599	arg1	monogalactosyldiacylglycerol					1572:1599	monogalactosyldiacylglycerol	1572:1599	monogalactosyldiacylglycerol	1572:1599	Chemotaxonomic data of strain NCCP-1664T showed the peptidoglycan type as A3α L-Lys-L -Ala; menaquinones as MK-9(H2) (67%), MK-8(H2) (32%) and MK-7(H2) (1%), major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids.
33459818	7	40	theme	monogalactosyldiacylglycerol	1572:1599	arg1	lipids					1651:1656	three unidentified lipids	1632:1656	three unidentified lipids	1632:1656	Chemotaxonomic data of strain NCCP-1664T showed the peptidoglycan type as A3α L-Lys-L -Ala; menaquinones as MK-9(H2) (67%), MK-8(H2) (32%) and MK-7(H2) (1%), major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids.
33459818	7	40	theme	monogalactosyldiacylglycerol	1572:1599	arg1	phosphatidylinositol					1505:1524	phosphatidylinositol	1505:1524	phosphatidylinositol	1505:1524	Chemotaxonomic data of strain NCCP-1664T showed the peptidoglycan type as A3α L-Lys-L -Ala; menaquinones as MK-9(H2) (67%), MK-8(H2) (32%) and MK-7(H2) (1%), major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids.
33459818	7	40	theme	monogalactosyldiacylglycerol	1572:1599	arg1	digalactosyldiacylglycerol					1527:1552	digalactosyldiacylglycerol	1527:1552	digalactosyldiacylglycerol	1527:1552	Chemotaxonomic data of strain NCCP-1664T showed the peptidoglycan type as A3α L-Lys-L -Ala; menaquinones as MK-9(H2) (67%), MK-8(H2) (32%) and MK-7(H2) (1%), major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids.
33459818	7	40	theme	monogalactosyldiacylglycerol	1572:1599	arg1	trimannosyldiacylglycerol					1602:1626	trimannosyldiacylglycerol	1602:1626	trimannosyldiacylglycerol	1602:1626	Chemotaxonomic data of strain NCCP-1664T showed the peptidoglycan type as A3α L-Lys-L -Ala; menaquinones as MK-9(H2) (67%), MK-8(H2) (32%) and MK-7(H2) (1%), major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids.
33459818	7	40	theme	monogalactosyldiacylglycerol	1572:1599	arg1	diphosphatidylglycerol					1459:1480	diphosphatidylglycerol	1459:1480	diphosphatidylglycerol	1459:1480	Chemotaxonomic data of strain NCCP-1664T showed the peptidoglycan type as A3α L-Lys-L -Ala; menaquinones as MK-9(H2) (67%), MK-8(H2) (32%) and MK-7(H2) (1%), major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids.
33459818	0	41	dep	sp	33:34	arg1	nov.					28:31	Zafaria cholistanensis gen. nov.	0:31	Zafaria cholistanensis gen. nov.	0:31	Zafaria cholistanensis gen. nov. sp.
33459818	8	42	theme	gen.	1861:1864	arg1	nov.					1866:1869	name Zafaria cholistanensis gen. nov.	1833:1869	the name Zafaria cholistanensis gen. nov. sp	1829:1872	The phylogenomic analyses along with chemotaxonomic data, physiological, biochemical characteristics allowed to describe it as representative of a novel genus, for which the name Zafaria cholistanensis gen. nov. sp.
33459818	4	43	theme	sequence	744:751	arg1	similarities					753:764	highest sequence similarities	736:764	highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%)	736:845	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
33459818	8	44	theme	novel	1806:1810	arg1	genus					1812:1816	a novel genus	1804:1816	a novel genus	1804:1816	The phylogenomic analyses along with chemotaxonomic data, physiological, biochemical characteristics allowed to describe it as representative of a novel genus, for which the name Zafaria cholistanensis gen. nov. sp.
33459818	4	45	contain	having	729:734	arg1	Arthrobacter					716:727	the genus Arthrobacter	706:727	the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa	706:887	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
33459818	4	45	contain	having	729:734	arg2	%					863:863	less than 97%	851:863	less than 97% with other related taxa	851:887	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
33459818	4	45	contain	having	729:734	arg2	similarities					753:764	highest sequence similarities	736:764	highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%)	736:845	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
33459818	6	46	theme	DNA	1122:1124	arg1	content					1132:1138	DNA G + C content	1122:1138	DNA G + C content of strain NCCP-1664T	1122:1159	DNA G + C content of strain NCCP-1664T was 70.0 mol%.
33459818	5	47	with	values	910:915	arg1	strains					964:970	above mentioned type strains	943:970	above mentioned type strains	943:970	DNA-DNA relatedness values of strain NCCP-1664T with above mentioned type strains were found to be less than 54%, whereas digital DDH and average nucleotide identity (ANI) values with A. oryzae were 20.9 and of 74.3%, respectively.
33459818	3	48	theme	optimum	494:500	arg1	28-45 °C					484:491	28-45 °C	484:491	28-45 °C (optimum 37 °C)	484:507	Cells of strain NCCP-1664T were strictly aerobic, catalase positive and oxidase negative with a rod to coccus growth cycle and can grow at pH 6.0-9.0 (optimum pH 7-8) at 28-45 °C (optimum 37 °C) and could tolerate 0-16% NaCl (optimum 2%).
33459818	3	48	theme	optimum	494:500	arg1	37 °C					502:506	optimum 37 °C	494:506	optimum 37 °C	494:506	Cells of strain NCCP-1664T were strictly aerobic, catalase positive and oxidase negative with a rod to coccus growth cycle and can grow at pH 6.0-9.0 (optimum pH 7-8) at 28-45 °C (optimum 37 °C) and could tolerate 0-16% NaCl (optimum 2%).
33459818	4	49	theme	rRNA	588:591	arg1	sequence					598:605	16S rRNA gene sequence	584:605	16S rRNA gene sequence	584:605	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
33459818	3	50	theme	0-16	528:531	arg1	%					532:532	%	532:532	%	532:532	Cells of strain NCCP-1664T were strictly aerobic, catalase positive and oxidase negative with a rod to coccus growth cycle and can grow at pH 6.0-9.0 (optimum pH 7-8) at 28-45 °C (optimum 37 °C) and could tolerate 0-16% NaCl (optimum 2%).
33459818	7	51	dep	showed	1217:1222	arg1	menaquinones					1268:1279	menaquinones	1268:1279	menaquinones as MK-9(H2) (67%), MK-8(H2) (32%) and MK-7(H2) (1%), major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids	1268:1656	Chemotaxonomic data of strain NCCP-1664T showed the peptidoglycan type as A3α L-Lys-L -Ala; menaquinones as MK-9(H2) (67%), MK-8(H2) (32%) and MK-7(H2) (1%), major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids.
33459818	7	52	theme	A3α	1250:1252	arg1	-Ala					1262:1265	A3α L-Lys-L -Ala	1250:1265	A3α L-Lys-L -Ala	1250:1265	Chemotaxonomic data of strain NCCP-1664T showed the peptidoglycan type as A3α L-Lys-L -Ala; menaquinones as MK-9(H2) (67%), MK-8(H2) (32%) and MK-7(H2) (1%), major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids.
33459818	9	53	theme	type	1901:1904	arg1	NCCP-1664T					1913:1922	the type strain NCCP-1664T	1897:1922	the type strain NCCP-1664T (= DSM 29936T = KCTC 39549T)	1897:1951	nov. is proposed with the type strain NCCP-1664T (= DSM 29936T = KCTC 39549T).
33459818	9	53	theme	type	1901:1904	arg1	39549T					1945:1950	= DSM 29936T = KCTC 39549T	1925:1950	= DSM 29936T = KCTC 39549T	1925:1950	nov. is proposed with the type strain NCCP-1664T (= DSM 29936T = KCTC 39549T).
33459818	7	54	theme	major	1334:1338	arg1	acids					1346:1350	major fatty acids	1334:1350	major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids	1334:1656	Chemotaxonomic data of strain NCCP-1664T showed the peptidoglycan type as A3α L-Lys-L -Ala; menaquinones as MK-9(H2) (67%), MK-8(H2) (32%) and MK-7(H2) (1%), major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids.
33459818	7	54	theme	major	1334:1338	arg1	MK-9					1284:1287	MK-9	1284:1287	MK-9(H2) (67%)	1284:1297	Chemotaxonomic data of strain NCCP-1664T showed the peptidoglycan type as A3α L-Lys-L -Ala; menaquinones as MK-9(H2) (67%), MK-8(H2) (32%) and MK-7(H2) (1%), major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids.
33459818	5	55	theme	type	959:962	arg1	strains					964:970	above mentioned type strains	943:970	above mentioned type strains	943:970	DNA-DNA relatedness values of strain NCCP-1664T with above mentioned type strains were found to be less than 54%, whereas digital DDH and average nucleotide identity (ANI) values with A. oryzae were 20.9 and of 74.3%, respectively.
33459818	2	56	theme	strain	249:254	arg1	NCCP-1664T					256:265	strain NCCP-1664T	249:265	strain NCCP-1664T	249:265	A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium, designated strain NCCP-1664T, was isolated from Cholistan desert, Pakistan.
33459818	5	57	dep	A.	1074:1075	arg1	oryzae					1077:1082	A. oryzae	1074:1082	A. oryzae	1074:1082	DNA-DNA relatedness values of strain NCCP-1664T with above mentioned type strains were found to be less than 54%, whereas digital DDH and average nucleotide identity (ANI) values with A. oryzae were 20.9 and of 74.3%, respectively.
33459818	9	58	theme	= DSM	1925:1929	arg1	NCCP-1664T					1913:1922	the type strain NCCP-1664T	1897:1922	the type strain NCCP-1664T (= DSM 29936T = KCTC 39549T)	1897:1951	nov. is proposed with the type strain NCCP-1664T (= DSM 29936T = KCTC 39549T).
33459818	9	58	theme	= DSM	1925:1929	arg1	39549T					1945:1950	= DSM 29936T = KCTC 39549T	1925:1950	= DSM 29936T = KCTC 39549T	1925:1950	nov. is proposed with the type strain NCCP-1664T (= DSM 29936T = KCTC 39549T).
33459818	6	59	theme	strain	1143:1148	arg1	NCCP-1664T					1150:1159	strain NCCP-1664T	1143:1159	strain NCCP-1664T	1143:1159	DNA G + C content of strain NCCP-1664T was 70.0 mol%.
33459818	1	60	theme	Cholistan	118:126	arg1	soil					135:138	Cholistan desert soil	118:138	Cholistan desert soil of Pakistan	118:150	nov., a moderately thermotolerant and halotolerant actinobacterium isolated from Cholistan desert soil of Pakistan.
33459818	3	61	with	positive	373:380	arg1	rod					410:412	a rod	408:412	a rod to coccus growth cycle	408:435	Cells of strain NCCP-1664T were strictly aerobic, catalase positive and oxidase negative with a rod to coccus growth cycle and can grow at pH 6.0-9.0 (optimum pH 7-8) at 28-45 °C (optimum 37 °C) and could tolerate 0-16% NaCl (optimum 2%).
33459818	1	62	attach	isolated	104:111	arg1	soil					135:138	Cholistan desert soil	118:138	Cholistan desert soil of Pakistan	118:150	nov., a moderately thermotolerant and halotolerant actinobacterium isolated from Cholistan desert soil of Pakistan.
33459818	1	62	attach	isolated	104:111	arg2	actinobacterium					88:102	a moderately thermotolerant and halotolerant actinobacterium	43:102	a moderately thermotolerant and halotolerant actinobacterium isolated from Cholistan desert soil of Pakistan	43:150	nov., a moderately thermotolerant and halotolerant actinobacterium isolated from Cholistan desert soil of Pakistan.
33459818	5	63	theme	NCCP-1664T	927:936	arg1	values					910:915	DNA-DNA relatedness values	890:915	DNA-DNA relatedness values of strain NCCP-1664T with above mentioned type strains	890:970	DNA-DNA relatedness values of strain NCCP-1664T with above mentioned type strains were found to be less than 54%, whereas digital DDH and average nucleotide identity (ANI) values with A. oryzae were 20.9 and of 74.3%, respectively.
33459818	4	64	theme	family	654:659	arg1	Micrococcaceae					661:674	the family Micrococcaceae	650:674	the family Micrococcaceae	650:674	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
33459818	5	65	theme	above	943:947	arg1	strains					964:970	above mentioned type strains	943:970	above mentioned type strains	943:970	DNA-DNA relatedness values of strain NCCP-1664T with above mentioned type strains were found to be less than 54%, whereas digital DDH and average nucleotide identity (ANI) values with A. oryzae were 20.9 and of 74.3%, respectively.
33459818	1	66	theme	Pakistan	143:150	arg1	soil					135:138	Cholistan desert soil	118:138	Cholistan desert soil of Pakistan	118:150	nov., a moderately thermotolerant and halotolerant actinobacterium isolated from Cholistan desert soil of Pakistan.
33459818	7	67	theme	Chemotaxonomic	1176:1189	arg1	data					1191:1194	Chemotaxonomic data	1176:1194	Chemotaxonomic data of strain NCCP-1664T	1176:1215	Chemotaxonomic data of strain NCCP-1664T showed the peptidoglycan type as A3α L-Lys-L -Ala; menaquinones as MK-9(H2) (67%), MK-8(H2) (32%) and MK-7(H2) (1%), major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids.
33459818	4	68	with	similarities	753:764	arg1	taxa					884:887	other related taxa	870:887	other related taxa	870:887	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
33459818	4	68	with	similarities	753:764	arg1	oryzae					832:837	A. oryzae	829:837	A. oryzae (97.1%)	829:845	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
33459818	4	68	with	similarities	753:764	arg1	%					799:799	98.9%	795:799	98.9%	795:799	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
33459818	4	68	with	similarities	753:764	arg1	%					822:822	97.7%	818:822	97.7%	818:822	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
33459818	4	68	with	similarities	753:764	arg1	%					844:844	97.1%	840:844	97.1%	840:844	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
33459818	4	68	with	similarities	753:764	arg1	ginkgonis					784:792	Arthrobacter ginkgonis	771:792	Arthrobacter ginkgonis (98.9%)	771:800	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
33459818	4	68	with	similarities	753:764	arg1	A.					803:804	A.	803:804	A.	803:804	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
33459818	8	69	theme	physiological	1717:1729	arg1	characteristics					1744:1758	physiological, biochemical characteristics	1717:1758	physiological, biochemical characteristics	1717:1758	The phylogenomic analyses along with chemotaxonomic data, physiological, biochemical characteristics allowed to describe it as representative of a novel genus, for which the name Zafaria cholistanensis gen. nov. sp.
33459818	8	69	theme	physiological	1717:1729	arg1	data					1711:1714	chemotaxonomic data	1696:1714	chemotaxonomic data	1696:1714	The phylogenomic analyses along with chemotaxonomic data, physiological, biochemical characteristics allowed to describe it as representative of a novel genus, for which the name Zafaria cholistanensis gen. nov. sp.
33459818	3	70	theme	NCCP-1664T	330:339	arg1	Cells					314:318	Cells	314:318	Cells of strain NCCP-1664T	314:339	Cells of strain NCCP-1664T were strictly aerobic, catalase positive and oxidase negative with a rod to coccus growth cycle and can grow at pH 6.0-9.0 (optimum pH 7-8) at 28-45 °C (optimum 37 °C) and could tolerate 0-16% NaCl (optimum 2%).
33459818	1	71	dep	actinobacterium	88:102	arg1	nov.					37:40	nov.	37:40	nov.	37:40	nov., a moderately thermotolerant and halotolerant actinobacterium isolated from Cholistan desert soil of Pakistan.
33459818	1	72	theme	thermotolerant	56:69	arg1	actinobacterium					88:102	a moderately thermotolerant and halotolerant actinobacterium	43:102	a moderately thermotolerant and halotolerant actinobacterium isolated from Cholistan desert soil of Pakistan	43:150	nov., a moderately thermotolerant and halotolerant actinobacterium isolated from Cholistan desert soil of Pakistan.
33459818	0	73	theme	cholistanensis	8:21	arg1	nov.					28:31	Zafaria cholistanensis gen. nov.	0:31	Zafaria cholistanensis gen. nov.	0:31	Zafaria cholistanensis gen. nov. sp.
33459818	7	74	theme	unidentified	1638:1649	arg1	lipids					1651:1656	three unidentified lipids	1632:1656	three unidentified lipids	1632:1656	Chemotaxonomic data of strain NCCP-1664T showed the peptidoglycan type as A3α L-Lys-L -Ala; menaquinones as MK-9(H2) (67%), MK-8(H2) (32%) and MK-7(H2) (1%), major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids.
33459818	1	75	theme	halotolerant	75:86	arg1	actinobacterium					88:102	a moderately thermotolerant and halotolerant actinobacterium	43:102	a moderately thermotolerant and halotolerant actinobacterium isolated from Cholistan desert soil of Pakistan	43:150	nov., a moderately thermotolerant and halotolerant actinobacterium isolated from Cholistan desert soil of Pakistan.
33459818	5	76	theme	nucleotide	1036:1045	arg1	identity					1047:1054	average nucleotide identity	1028:1054	average nucleotide identity	1028:1054	DNA-DNA relatedness values of strain NCCP-1664T with above mentioned type strains were found to be less than 54%, whereas digital DDH and average nucleotide identity (ANI) values with A. oryzae were 20.9 and of 74.3%, respectively.
33459818	3	77	theme	optimum	540:546	arg1	NaCl					534:537	0-16% NaCl	528:537	0-16% NaCl (optimum 2%)	528:550	Cells of strain NCCP-1664T were strictly aerobic, catalase positive and oxidase negative with a rod to coccus growth cycle and can grow at pH 6.0-9.0 (optimum pH 7-8) at 28-45 °C (optimum 37 °C) and could tolerate 0-16% NaCl (optimum 2%).
33459818	3	77	theme	optimum	540:546	arg1	%					549:549	optimum 2%	540:549	optimum 2%	540:549	Cells of strain NCCP-1664T were strictly aerobic, catalase positive and oxidase negative with a rod to coccus growth cycle and can grow at pH 6.0-9.0 (optimum pH 7-8) at 28-45 °C (optimum 37 °C) and could tolerate 0-16% NaCl (optimum 2%).
33459818	8	78	theme	name	1833:1836	arg1	nov.					1866:1869	name Zafaria cholistanensis gen. nov.	1833:1869	the name Zafaria cholistanensis gen. nov. sp	1829:1872	The phylogenomic analyses along with chemotaxonomic data, physiological, biochemical characteristics allowed to describe it as representative of a novel genus, for which the name Zafaria cholistanensis gen. nov. sp.
33459818	2	79	theme	forming	189:195	arg1	bacterium					227:235	A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium	153:235	A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium	153:235	A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium, designated strain NCCP-1664T, was isolated from Cholistan desert, Pakistan.
33459818	5	80	theme	DNA-DNA	890:896	arg1	values					910:915	DNA-DNA relatedness values	890:915	DNA-DNA relatedness values of strain NCCP-1664T with above mentioned type strains	890:970	DNA-DNA relatedness values of strain NCCP-1664T with above mentioned type strains were found to be less than 54%, whereas digital DDH and average nucleotide identity (ANI) values with A. oryzae were 20.9 and of 74.3%, respectively.
33459818	8	81	theme	cholistanensis	1846:1859	arg1	nov.					1866:1869	name Zafaria cholistanensis gen. nov.	1833:1869	the name Zafaria cholistanensis gen. nov. sp	1829:1872	The phylogenomic analyses along with chemotaxonomic data, physiological, biochemical characteristics allowed to describe it as representative of a novel genus, for which the name Zafaria cholistanensis gen. nov. sp.
33459818	3	82	theme	coccus	417:422	arg1	cycle					431:435	coccus growth cycle	417:435	coccus growth cycle	417:435	Cells of strain NCCP-1664T were strictly aerobic, catalase positive and oxidase negative with a rod to coccus growth cycle and can grow at pH 6.0-9.0 (optimum pH 7-8) at 28-45 °C (optimum 37 °C) and could tolerate 0-16% NaCl (optimum 2%).
33459818	5	83	with	values	1062:1067	arg1	A.					1074:1075	A.	1074:1075	A.	1074:1075	DNA-DNA relatedness values of strain NCCP-1664T with above mentioned type strains were found to be less than 54%, whereas digital DDH and average nucleotide identity (ANI) values with A. oryzae were 20.9 and of 74.3%, respectively.
33459818	4	84	dep	A.	803:804	arg1	halodurans					806:815	A. halodurans (97.7%)	803:823	A. halodurans (97.7%)	803:823	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
33459818	5	85	theme	digital	1012:1018	arg1	DDH					1020:1022	digital DDH	1012:1022	digital DDH	1012:1022	DNA-DNA relatedness values of strain NCCP-1664T with above mentioned type strains were found to be less than 54%, whereas digital DDH and average nucleotide identity (ANI) values with A. oryzae were 20.9 and of 74.3%, respectively.
33459818	5	85	theme	digital	1012:1018	arg1	ANI					1057:1059	ANI	1057:1059	ANI	1057:1059	DNA-DNA relatedness values of strain NCCP-1664T with above mentioned type strains were found to be less than 54%, whereas digital DDH and average nucleotide identity (ANI) values with A. oryzae were 20.9 and of 74.3%, respectively.
33459818	4	86	with	%	863:863	arg1	taxa					884:887	other related taxa	870:887	other related taxa	870:887	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
33459818	4	86	with	%	863:863	arg1	oryzae					832:837	A. oryzae	829:837	A. oryzae (97.1%)	829:845	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
33459818	4	86	with	%	863:863	arg1	%					799:799	98.9%	795:799	98.9%	795:799	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
33459818	4	86	with	%	863:863	arg1	%					822:822	97.7%	818:822	97.7%	818:822	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
33459818	4	86	with	%	863:863	arg1	%					844:844	97.1%	840:844	97.1%	840:844	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
33459818	4	86	with	%	863:863	arg1	ginkgonis					784:792	Arthrobacter ginkgonis	771:792	Arthrobacter ginkgonis (98.9%)	771:800	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
33459818	4	86	with	%	863:863	arg1	A.					803:804	A.	803:804	A.	803:804	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
33459818	8	87	theme	nov.	1866:1869	arg1	sp					1871:1872	the name Zafaria cholistanensis gen. nov. sp	1829:1872	the name Zafaria cholistanensis gen. nov. sp	1829:1872	The phylogenomic analyses along with chemotaxonomic data, physiological, biochemical characteristics allowed to describe it as representative of a novel genus, for which the name Zafaria cholistanensis gen. nov. sp.
33459818	5	88	dep	found	977:981	arg1	whereas					1004:1010	whereas	1004:1010	whereas	1004:1010	DNA-DNA relatedness values of strain NCCP-1664T with above mentioned type strains were found to be less than 54%, whereas digital DDH and average nucleotide identity (ANI) values with A. oryzae were 20.9 and of 74.3%, respectively.
33459818	2	89	theme	non-motile	216:225	arg1	bacterium					227:235	A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium	153:235	A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium	153:235	A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium, designated strain NCCP-1664T, was isolated from Cholistan desert, Pakistan.
33459818	7	90	theme	small	1555:1559	arg1	monogalactosyldiacylglycerol					1572:1599	monogalactosyldiacylglycerol	1572:1599	monogalactosyldiacylglycerol	1572:1599	Chemotaxonomic data of strain NCCP-1664T showed the peptidoglycan type as A3α L-Lys-L -Ala; menaquinones as MK-9(H2) (67%), MK-8(H2) (32%) and MK-7(H2) (1%), major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids.
33459818	7	90	theme	small	1555:1559	arg1	amounts					1561:1567	small amounts	1555:1567	small amounts of monogalactosyldiacylglycerol	1555:1599	Chemotaxonomic data of strain NCCP-1664T showed the peptidoglycan type as A3α L-Lys-L -Ala; menaquinones as MK-9(H2) (67%), MK-8(H2) (32%) and MK-7(H2) (1%), major fatty acids as anteiso -C15:0 (51.2%), anteiso-C17:0 (9.6%) and C18:1ω9c (6.9%) and polar lipids profile comprising of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, digalactosyldiacylglycerol, small amounts of monogalactosyldiacylglycerol, trimannosyldiacylglycerol and three unidentified lipids.
33459818	2	91	theme	non-pigmented	198:210	arg1	bacterium					227:235	A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium	153:235	A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium	153:235	A Gram-staining positive, non-spore forming, non-pigmented and non-motile bacterium, designated strain NCCP-1664T, was isolated from Cholistan desert, Pakistan.
33459818	4	92	theme	Arthrobacter	716:727	arg1	members					695:701	members	695:701	members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa	695:887	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
33459818	3	93	theme	optimum	465:471	arg1	pH					473:474	optimum pH 7-8	465:478	optimum pH 7-8	465:478	Cells of strain NCCP-1664T were strictly aerobic, catalase positive and oxidase negative with a rod to coccus growth cycle and can grow at pH 6.0-9.0 (optimum pH 7-8) at 28-45 °C (optimum 37 °C) and could tolerate 0-16% NaCl (optimum 2%).
33459818	3	93	theme	optimum	465:471	arg1	pH					453:454	pH 6.0-9.0	453:462	pH 6.0-9.0 (optimum pH 7-8)	453:479	Cells of strain NCCP-1664T were strictly aerobic, catalase positive and oxidase negative with a rod to coccus growth cycle and can grow at pH 6.0-9.0 (optimum pH 7-8) at 28-45 °C (optimum 37 °C) and could tolerate 0-16% NaCl (optimum 2%).
33459818	4	94	theme	Phylogenetic	553:564	arg1	analyses					566:573	Phylogenetic analyses	553:573	Phylogenetic analyses based on 16S rRNA gene sequence	553:605	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
33459818	4	95	theme	highest	736:742	arg1	similarities					753:764	highest sequence similarities	736:764	highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%)	736:845	Phylogenetic analyses based on 16S rRNA gene sequence revealed that strain NCCP-1664T belongs to the family Micrococcaceae and was related to members of the genus Arthrobacter having highest sequence similarities with Arthrobacter ginkgonis (98.9%), A. halodurans (97.7%) and A. oryzae (97.1%) and less than 97% with other related taxa.
34872351	11	0	theme	hyperexpression	1749:1763	arg1	impact					1732:1737	the impact	1728:1737	the impact of OXA-23 hyperexpression on peptidoglycan integrity	1728:1790	Our results highlight the impact of OXA-23 hyperexpression on peptidoglycan integrity and reveal new genetic vulnerabilities, which may represent novel targets for antimicrobial agents specific to MDR A. baumannii and other OXA β-lactamase-overexpressing Enterobacteriaceae, while having no impact on the normal flora.
34872351	1	1	theme	Acinetobacter	283:295	arg1	baumannii					297:305	the multidrug-resistant (MDR) bacterium Acinetobacter baumannii	243:305	the multidrug-resistant (MDR) bacterium Acinetobacter baumannii	243:305	β-Lactamase expression is the major mechanism of resistance to penicillins, cephalosporins, and carbapenems in the multidrug-resistant (MDR) bacterium Acinetobacter baumannii.
34872351	3	2	contain	have	602:605	arg2	function					618:625	a cellular function	607:625	a cellular function	607:625	Moreover, the OXA-51 β-lactamase is universally present in the A. baumannii chromosome, suggesting it may have a cellular function beyond antibiotic resistance.
34872351	3	2	contain	have	602:605	arg1	it					595:596	it	595:596	it	595:596	Moreover, the OXA-51 β-lactamase is universally present in the A. baumannii chromosome, suggesting it may have a cellular function beyond antibiotic resistance.
34872351	11	3	theme	new	1803:1805	arg1	vulnerabilities					1815:1829	new genetic vulnerabilities	1803:1829	new genetic vulnerabilities	1803:1829	Our results highlight the impact of OXA-23 hyperexpression on peptidoglycan integrity and reveal new genetic vulnerabilities, which may represent novel targets for antimicrobial agents specific to MDR A. baumannii and other OXA β-lactamase-overexpressing Enterobacteriaceae, while having no impact on the normal flora.
34872351	13	4	theme	β-lactam	2135:2142	arg1	option					2188:2193	a primary treatment option	2168:2193	a primary treatment option for A. baumannii infections	2168:2221	The β-lactam class of antibiotics is a primary treatment option for A. baumannii infections, and expression of β-lactamases is the most frequent mechanism of resistance in this bacterium.
34872351	13	4	theme	β-lactam	2135:2142	arg1	class					2144:2148	The β-lactam class	2131:2148	The β-lactam class of antibiotics	2131:2163	The β-lactam class of antibiotics is a primary treatment option for A. baumannii infections, and expression of β-lactamases is the most frequent mechanism of resistance in this bacterium.
34872351	17	5	theme	β-lactamase-expressing	2967:2988	arg1	strains					2990:2996	β-lactamase-expressing strains	2967:2996	β-lactamase-expressing strains	2967:2996	This work paves the way for the identification of new antimicrobial targets, where inhibitors would selectively kill β-lactamase-expressing strains.
34872351	14	6	theme	A.	2366:2367	arg1	strains					2379:2385	multidrug-resistant A. baumannii strains	2346:2385	multidrug-resistant A. baumannii strains	2346:2385	New approaches to treating multidrug-resistant A. baumannii strains are needed.
34872351	5	7	theme	significant	898:908	arg1	changes					921:927	significant collateral changes	898:927	significant collateral changes	898:927	Using peptidoglycan composition analysis, we show that overexpressing the OXA-23 β-lactamase in A. baumannii drives significant collateral changes with alterations consistent with increased amidase activity.
34872351	17	8	theme	targets	2918:2924	arg1	identification					2882:2895	the identification	2878:2895	the identification of new antimicrobial targets, where inhibitors would selectively kill β-lactamase-expressing strains	2878:2996	This work paves the way for the identification of new antimicrobial targets, where inhibitors would selectively kill β-lactamase-expressing strains.
34872351	11	9	theme	β-lactamase-overexpressing	1934:1959	arg1	Enterobacteriaceae					1961:1978	other OXA β-lactamase-overexpressing Enterobacteriaceae	1924:1978	other OXA β-lactamase-overexpressing Enterobacteriaceae	1924:1978	Our results highlight the impact of OXA-23 hyperexpression on peptidoglycan integrity and reveal new genetic vulnerabilities, which may represent novel targets for antimicrobial agents specific to MDR A. baumannii and other OXA β-lactamase-overexpressing Enterobacteriaceae, while having no impact on the normal flora.
34872351	9	10	theme	peptidoglycan	1548:1560	arg1	MurA					1579:1582	the essential peptidoglycan synthesis enzyme MurA	1534:1582	the essential peptidoglycan synthesis enzyme MurA	1534:1582	In addition, we demonstrated a synergistic lethal relationship between OXA-23 overexpression and a CRISPR interference (CRISPRi) knockdown of the essential peptidoglycan synthesis enzyme MurA.
34872351	16	11	theme	synthesis	2839:2847	arg1	inhibition					2811:2820	genetic and chemical inhibition	2790:2820	genetic and chemical inhibition of peptidoglycan synthesis	2790:2847	We have identified genes that become selectively essential in OXA-23-expressing strains and confirmed the relationship between altered peptidoglycan and OXA-23 expression by demonstrating that OXA-23 overexpression sensitizes cells to genetic and chemical inhibition of peptidoglycan synthesis.
34872351	11	12	theme	other	1924:1928	arg1	Enterobacteriaceae					1961:1978	other OXA β-lactamase-overexpressing Enterobacteriaceae	1924:1978	other OXA β-lactamase-overexpressing Enterobacteriaceae	1924:1978	Our results highlight the impact of OXA-23 hyperexpression on peptidoglycan integrity and reveal new genetic vulnerabilities, which may represent novel targets for antimicrobial agents specific to MDR A. baumannii and other OXA β-lactamase-overexpressing Enterobacteriaceae, while having no impact on the normal flora.
34872351	7	13	theme	insertions	1132:1141	arg1	screen					1104:1109	a small screen	1096:1109	a small screen of random transposon insertions	1096:1141	As proof of principle, a small screen of random transposon insertions revealed three genes, where mutations resulted in a greater than 19-fold loss of viability when OXA-23 was overexpressed.
34872351	2	14	theme	high-level	324:333	arg1	expression					335:344	stable high-level expression	317:344	stable high-level expression of at least one β-lactamase	317:372	In fact, stable high-level expression of at least one β-lactamase has been rapidly increasing and reported to occur in up to 98.5% of modern A. baumannii isolates recovered in the clinic.
34872351	3	15	located	present	544:550	arg2	β-lactamase					517:527	the OXA-51 β-lactamase	506:527	the OXA-51 β-lactamase	506:527	Moreover, the OXA-51 β-lactamase is universally present in the A. baumannii chromosome, suggesting it may have a cellular function beyond antibiotic resistance.
34872351	3	15	located	present	544:550	arg1	chromosome					572:581	the A. baumannii chromosome	555:581	the A. baumannii chromosome	555:581	Moreover, the OXA-51 β-lactamase is universally present in the A. baumannii chromosome, suggesting it may have a cellular function beyond antibiotic resistance.
34872351	3	15	located	present	544:550	arg2	present					544:550	present	544:550	present	544:550	Moreover, the OXA-51 β-lactamase is universally present in the A. baumannii chromosome, suggesting it may have a cellular function beyond antibiotic resistance.
34872351	9	16	theme	enzyme	1572:1577	arg1	MurA					1579:1582	the essential peptidoglycan synthesis enzyme MurA	1534:1582	the essential peptidoglycan synthesis enzyme MurA	1534:1582	In addition, we demonstrated a synergistic lethal relationship between OXA-23 overexpression and a CRISPR interference (CRISPRi) knockdown of the essential peptidoglycan synthesis enzyme MurA.
34872351	8	17	theme	identified	1269:1278	arg1	genes					1280:1284	The identified genes	1265:1284	The identified genes	1265:1284	The identified genes remained conditionally essential even when a catalytically inactive OXA-23 β-lactamase was overexpressed.
34872351	8	18	theme	OXA-23	1354:1359	arg1	β-lactamase					1361:1371	a catalytically inactive OXA-23 β-lactamase	1329:1371	a catalytically inactive OXA-23 β-lactamase	1329:1371	The identified genes remained conditionally essential even when a catalytically inactive OXA-23 β-lactamase was overexpressed.
34872351	7	19	theme	random	1114:1119	arg1	insertions					1132:1141	random transposon insertions	1114:1141	random transposon insertions	1114:1141	As proof of principle, a small screen of random transposon insertions revealed three genes, where mutations resulted in a greater than 19-fold loss of viability when OXA-23 was overexpressed.
34872351	13	20	theme	resistance	2289:2298	arg1	expression					2228:2237	expression	2228:2237	expression of β-lactamases	2228:2253	The β-lactam class of antibiotics is a primary treatment option for A. baumannii infections, and expression of β-lactamases is the most frequent mechanism of resistance in this bacterium.
34872351	13	20	theme	resistance	2289:2298	arg1	option					2188:2193	a primary treatment option	2168:2193	a primary treatment option for A. baumannii infections	2168:2221	The β-lactam class of antibiotics is a primary treatment option for A. baumannii infections, and expression of β-lactamases is the most frequent mechanism of resistance in this bacterium.
34872351	13	20	theme	resistance	2289:2298	arg1	mechanism					2276:2284	the most frequent mechanism	2258:2284	the most frequent mechanism of resistance in this bacterium	2258:2316	The β-lactam class of antibiotics is a primary treatment option for A. baumannii infections, and expression of β-lactamases is the most frequent mechanism of resistance in this bacterium.
34872351	13	20	theme	resistance	2289:2298	arg1	class					2144:2148	The β-lactam class	2131:2148	The β-lactam class of antibiotics	2131:2163	The β-lactam class of antibiotics is a primary treatment option for A. baumannii infections, and expression of β-lactamases is the most frequent mechanism of resistance in this bacterium.
34872351	16	21	from	essential	2604:2612	arg1	strains					2635:2641	OXA-23-expressing strains	2617:2641	OXA-23-expressing strains	2617:2641	We have identified genes that become selectively essential in OXA-23-expressing strains and confirmed the relationship between altered peptidoglycan and OXA-23 expression by demonstrating that OXA-23 overexpression sensitizes cells to genetic and chemical inhibition of peptidoglycan synthesis.
34872351	17	22	theme	antimicrobial	2904:2916	arg1	targets					2918:2924	new antimicrobial targets	2900:2924	new antimicrobial targets	2900:2924	This work paves the way for the identification of new antimicrobial targets, where inhibitors would selectively kill β-lactamase-expressing strains.
34872351	4	23	theme	A.	733:734	arg1	physiology					746:755	A. baumannii physiology	733:755	A. baumannii physiology	733:755	However, the consequences associated with OXA β-lactamase overexpression on A. baumannii physiology are not well understood.
34872351	11	24	dep	MDR	1903:1905	arg1	baumannii					1910:1918	MDR A. baumannii	1903:1918	MDR A. baumannii	1903:1918	Our results highlight the impact of OXA-23 hyperexpression on peptidoglycan integrity and reveal new genetic vulnerabilities, which may represent novel targets for antimicrobial agents specific to MDR A. baumannii and other OXA β-lactamase-overexpressing Enterobacteriaceae, while having no impact on the normal flora.
34872351	5	25	theme	amidase	972:978	arg1	activity					980:987	increased amidase activity	962:987	increased amidase activity	962:987	Using peptidoglycan composition analysis, we show that overexpressing the OXA-23 β-lactamase in A. baumannii drives significant collateral changes with alterations consistent with increased amidase activity.
34872351	11	26	theme	specific	1891:1898	arg1	agents					1884:1889	antimicrobial agents	1870:1889	antimicrobial agents specific to MDR A. baumannii and other OXA β-lactamase-overexpressing Enterobacteriaceae	1870:1978	Our results highlight the impact of OXA-23 hyperexpression on peptidoglycan integrity and reveal new genetic vulnerabilities, which may represent novel targets for antimicrobial agents specific to MDR A. baumannii and other OXA β-lactamase-overexpressing Enterobacteriaceae, while having no impact on the normal flora.
34872351	15	27	theme	peptidoglycan	2519:2531	arg1	structure					2533:2541	peptidoglycan structure	2519:2541	peptidoglycan structure	2519:2541	In this study, we demonstrate that overexpressing the OXA-23 β-lactamase leads to significant collateral changes, where peptidoglycan structure is altered.
34872351	1	28	theme	major	162:166	arg1	expression					144:153	β-Lactamase expression	132:153	β-Lactamase expression	132:153	β-Lactamase expression is the major mechanism of resistance to penicillins, cephalosporins, and carbapenems in the multidrug-resistant (MDR) bacterium Acinetobacter baumannii.
34872351	1	28	theme	major	162:166	arg1	mechanism					168:176	the major mechanism	158:176	the major mechanism of resistance to penicillins, cephalosporins, and carbapenems in the multidrug-resistant (MDR) bacterium Acinetobacter baumannii	158:305	β-Lactamase expression is the major mechanism of resistance to penicillins, cephalosporins, and carbapenems in the multidrug-resistant (MDR) bacterium Acinetobacter baumannii.
34872351	4	29	theme	OXA	699:701	arg1	overexpression					715:728	OXA β-lactamase overexpression	699:728	OXA β-lactamase overexpression on A. baumannii physiology	699:755	However, the consequences associated with OXA β-lactamase overexpression on A. baumannii physiology are not well understood.
34872351	11	30	theme	antimicrobial	1870:1882	arg1	agents					1884:1889	antimicrobial agents	1870:1889	antimicrobial agents specific to MDR A. baumannii and other OXA β-lactamase-overexpressing Enterobacteriaceae	1870:1978	Our results highlight the impact of OXA-23 hyperexpression on peptidoglycan integrity and reveal new genetic vulnerabilities, which may represent novel targets for antimicrobial agents specific to MDR A. baumannii and other OXA β-lactamase-overexpressing Enterobacteriaceae, while having no impact on the normal flora.
34872351	3	31	theme	cellular	609:616	arg1	function					618:625	a cellular function	607:625	a cellular function	607:625	Moreover, the OXA-51 β-lactamase is universally present in the A. baumannii chromosome, suggesting it may have a cellular function beyond antibiotic resistance.
34872351	16	32	theme	genetic	2790:2796	arg1	inhibition					2811:2820	genetic and chemical inhibition	2790:2820	genetic and chemical inhibition of peptidoglycan synthesis	2790:2847	We have identified genes that become selectively essential in OXA-23-expressing strains and confirmed the relationship between altered peptidoglycan and OXA-23 expression by demonstrating that OXA-23 overexpression sensitizes cells to genetic and chemical inhibition of peptidoglycan synthesis.
34872351	0	33	from	Overexpression	19:32	arg1	baumannii					51:59	Acinetobacter baumannii	37:59	Acinetobacter baumannii	37:59	OXA-23 β-Lactamase Overexpression in Acinetobacter baumannii Drives Physiological Changes Resulting in New Genetic Vulnerabilities.
34872351	5	34	with	consistent	946:955	arg1	activity					980:987	increased amidase activity	962:987	increased amidase activity	962:987	Using peptidoglycan composition analysis, we show that overexpressing the OXA-23 β-lactamase in A. baumannii drives significant collateral changes with alterations consistent with increased amidase activity.
34872351	3	35	from	present	544:550	arg1	chromosome					572:581	the A. baumannii chromosome	555:581	the A. baumannii chromosome	555:581	Moreover, the OXA-51 β-lactamase is universally present in the A. baumannii chromosome, suggesting it may have a cellular function beyond antibiotic resistance.
34872351	5	36	theme	composition	802:812	arg1	analysis					814:821	peptidoglycan composition analysis	788:821	peptidoglycan composition analysis	788:821	Using peptidoglycan composition analysis, we show that overexpressing the OXA-23 β-lactamase in A. baumannii drives significant collateral changes with alterations consistent with increased amidase activity.
34872351	12	37	theme	serious	2073:2079	arg1	pathogen					2081:2088	a serious pathogen	2071:2088	a serious pathogen in both hospital and community settings	2071:2128	IMPORTANCE Acinetobacter baumannii has become a serious pathogen in both hospital and community settings.
34872351	1	38	from	resistance	181:190	arg1	baumannii					297:305	the multidrug-resistant (MDR) bacterium Acinetobacter baumannii	243:305	the multidrug-resistant (MDR) bacterium Acinetobacter baumannii	243:305	β-Lactamase expression is the major mechanism of resistance to penicillins, cephalosporins, and carbapenems in the multidrug-resistant (MDR) bacterium Acinetobacter baumannii.
34872351	16	39	theme	chemical	2802:2809	arg1	inhibition					2811:2820	genetic and chemical inhibition	2790:2820	genetic and chemical inhibition of peptidoglycan synthesis	2790:2847	We have identified genes that become selectively essential in OXA-23-expressing strains and confirmed the relationship between altered peptidoglycan and OXA-23 expression by demonstrating that OXA-23 overexpression sensitizes cells to genetic and chemical inhibition of peptidoglycan synthesis.
34872351	0	40	theme	Genetic	107:113	arg1	Vulnerabilities					115:129	New Genetic Vulnerabilities	103:129	New Genetic Vulnerabilities	103:129	OXA-23 β-Lactamase Overexpression in Acinetobacter baumannii Drives Physiological Changes Resulting in New Genetic Vulnerabilities.
34872351	13	41	theme	A.	2199:2200	arg1	infections					2212:2221	A. baumannii infections	2199:2221	A. baumannii infections	2199:2221	The β-lactam class of antibiotics is a primary treatment option for A. baumannii infections, and expression of β-lactamases is the most frequent mechanism of resistance in this bacterium.
34872351	16	42	from	strains	2635:2641	arg1	essential					2604:2612	essential	2604:2612	essential	2604:2612	We have identified genes that become selectively essential in OXA-23-expressing strains and confirmed the relationship between altered peptidoglycan and OXA-23 expression by demonstrating that OXA-23 overexpression sensitizes cells to genetic and chemical inhibition of peptidoglycan synthesis.
34872351	1	43	from	mechanism	168:176	arg1	baumannii					297:305	the multidrug-resistant (MDR) bacterium Acinetobacter baumannii	243:305	the multidrug-resistant (MDR) bacterium Acinetobacter baumannii	243:305	β-Lactamase expression is the major mechanism of resistance to penicillins, cephalosporins, and carbapenems in the multidrug-resistant (MDR) bacterium Acinetobacter baumannii.
34872351	6	44	theme	new	1043:1045	arg1	vulnerabilities					1056:1070	new cellular vulnerabilities	1043:1070	new cellular vulnerabilities	1043:1070	Consequently, we predicted that these changes create new cellular vulnerabilities.
34872351	14	45	theme	baumannii	2369:2377	arg1	strains					2379:2385	multidrug-resistant A. baumannii strains	2346:2385	multidrug-resistant A. baumannii strains	2346:2385	New approaches to treating multidrug-resistant A. baumannii strains are needed.
34872351	0	46	theme	OXA-23	0:5	arg1	Overexpression					19:32	OXA-23 β-Lactamase Overexpression	0:32	OXA-23 β-Lactamase Overexpression in Acinetobacter baumannii	0:59	OXA-23 β-Lactamase Overexpression in Acinetobacter baumannii Drives Physiological Changes Resulting in New Genetic Vulnerabilities.
34872351	2	47	dep	A.	449:450	arg1	baumannii					452:460	baumannii	452:460	baumannii	452:460	In fact, stable high-level expression of at least one β-lactamase has been rapidly increasing and reported to occur in up to 98.5% of modern A. baumannii isolates recovered in the clinic.
34872351	2	48	theme	modern	442:447	arg1	isolates					462:469	modern A. baumannii isolates	442:469	modern A. baumannii isolates recovered in the clinic	442:493	In fact, stable high-level expression of at least one β-lactamase has been rapidly increasing and reported to occur in up to 98.5% of modern A. baumannii isolates recovered in the clinic.
34872351	5	49	theme	OXA-23	856:861	arg1	β-lactamase					863:873	the OXA-23 β-lactamase	852:873	the OXA-23 β-lactamase	852:873	Using peptidoglycan composition analysis, we show that overexpressing the OXA-23 β-lactamase in A. baumannii drives significant collateral changes with alterations consistent with increased amidase activity.
34872351	9	50	theme	synergistic	1423:1433	arg1	relationship					1442:1453	a synergistic lethal relationship	1421:1453	a synergistic lethal relationship between OXA-23 overexpression and a CRISPR interference (CRISPRi) knockdown of the essential peptidoglycan synthesis enzyme MurA	1421:1582	In addition, we demonstrated a synergistic lethal relationship between OXA-23 overexpression and a CRISPR interference (CRISPRi) knockdown of the essential peptidoglycan synthesis enzyme MurA.
34872351	1	51	theme	β-Lactamase	132:142	arg1	expression					144:153	β-Lactamase expression	132:153	β-Lactamase expression	132:153	β-Lactamase expression is the major mechanism of resistance to penicillins, cephalosporins, and carbapenems in the multidrug-resistant (MDR) bacterium Acinetobacter baumannii.
34872351	1	51	theme	β-Lactamase	132:142	arg1	mechanism					168:176	the major mechanism	158:176	the major mechanism of resistance to penicillins, cephalosporins, and carbapenems in the multidrug-resistant (MDR) bacterium Acinetobacter baumannii	158:305	β-Lactamase expression is the major mechanism of resistance to penicillins, cephalosporins, and carbapenems in the multidrug-resistant (MDR) bacterium Acinetobacter baumannii.
34872351	3	52	attach	present	544:550	arg2	β-lactamase					517:527	the OXA-51 β-lactamase	506:527	the OXA-51 β-lactamase	506:527	Moreover, the OXA-51 β-lactamase is universally present in the A. baumannii chromosome, suggesting it may have a cellular function beyond antibiotic resistance.
34872351	3	52	attach	present	544:550	arg1	chromosome					572:581	the A. baumannii chromosome	555:581	the A. baumannii chromosome	555:581	Moreover, the OXA-51 β-lactamase is universally present in the A. baumannii chromosome, suggesting it may have a cellular function beyond antibiotic resistance.
34872351	3	52	attach	present	544:550	arg2	present					544:550	present	544:550	present	544:550	Moreover, the OXA-51 β-lactamase is universally present in the A. baumannii chromosome, suggesting it may have a cellular function beyond antibiotic resistance.
34872351	0	53	theme	Acinetobacter	37:49	arg1	baumannii					51:59	Acinetobacter baumannii	37:59	Acinetobacter baumannii	37:59	OXA-23 β-Lactamase Overexpression in Acinetobacter baumannii Drives Physiological Changes Resulting in New Genetic Vulnerabilities.
34872351	7	54	theme	19-fold	1208:1214	arg1	loss					1216:1219	a greater than 19-fold loss	1193:1219	a greater than 19-fold loss of viability	1193:1232	As proof of principle, a small screen of random transposon insertions revealed three genes, where mutations resulted in a greater than 19-fold loss of viability when OXA-23 was overexpressed.
34872351	13	55	theme	treatment	2178:2186	arg1	option					2188:2193	a primary treatment option	2168:2193	a primary treatment option for A. baumannii infections	2168:2221	The β-lactam class of antibiotics is a primary treatment option for A. baumannii infections, and expression of β-lactamases is the most frequent mechanism of resistance in this bacterium.
34872351	13	55	theme	treatment	2178:2186	arg1	class					2144:2148	The β-lactam class	2131:2148	The β-lactam class of antibiotics	2131:2163	The β-lactam class of antibiotics is a primary treatment option for A. baumannii infections, and expression of β-lactamases is the most frequent mechanism of resistance in this bacterium.
34872351	12	56	theme	IMPORTANCE	2025:2034	arg1	baumannii					2050:2058	IMPORTANCE Acinetobacter baumannii	2025:2058	IMPORTANCE Acinetobacter baumannii	2025:2058	IMPORTANCE Acinetobacter baumannii has become a serious pathogen in both hospital and community settings.
34872351	9	57	theme	OXA-23	1463:1468	arg1	overexpression					1470:1483	OXA-23 overexpression	1463:1483	OXA-23 overexpression	1463:1483	In addition, we demonstrated a synergistic lethal relationship between OXA-23 overexpression and a CRISPR interference (CRISPRi) knockdown of the essential peptidoglycan synthesis enzyme MurA.
34872351	16	58	theme	OXA-23	2748:2753	arg1	overexpression					2755:2768	OXA-23 overexpression	2748:2768	OXA-23 overexpression	2748:2768	We have identified genes that become selectively essential in OXA-23-expressing strains and confirmed the relationship between altered peptidoglycan and OXA-23 expression by demonstrating that OXA-23 overexpression sensitizes cells to genetic and chemical inhibition of peptidoglycan synthesis.
34872351	1	59	theme	multidrug-resistant	247:265	arg1	baumannii					297:305	the multidrug-resistant (MDR) bacterium Acinetobacter baumannii	243:305	the multidrug-resistant (MDR) bacterium Acinetobacter baumannii	243:305	β-Lactamase expression is the major mechanism of resistance to penicillins, cephalosporins, and carbapenems in the multidrug-resistant (MDR) bacterium Acinetobacter baumannii.
34872351	10	60	theme	synthesis	1665:1673	arg1	d-cycloserine					1676:1688	d-cycloserine	1676:1688	d-cycloserine	1676:1688	Last, OXA-23 overexpression sensitized cells to two inhibitors of peptidoglycan synthesis, d-cycloserine and fosfomycin.
34872351	10	60	theme	synthesis	1665:1673	arg1	inhibitors					1637:1646	two inhibitors	1633:1646	two inhibitors of peptidoglycan synthesis	1633:1673	Last, OXA-23 overexpression sensitized cells to two inhibitors of peptidoglycan synthesis, d-cycloserine and fosfomycin.
34872351	10	60	theme	synthesis	1665:1673	arg1	fosfomycin					1694:1703	fosfomycin	1694:1703	fosfomycin	1694:1703	Last, OXA-23 overexpression sensitized cells to two inhibitors of peptidoglycan synthesis, d-cycloserine and fosfomycin.
34872351	11	61	theme	novel	1852:1856	arg1	targets					1858:1864	novel targets	1852:1864	novel targets for antimicrobial agents specific to MDR A. baumannii and other OXA β-lactamase-overexpressing Enterobacteriaceae	1852:1978	Our results highlight the impact of OXA-23 hyperexpression on peptidoglycan integrity and reveal new genetic vulnerabilities, which may represent novel targets for antimicrobial agents specific to MDR A. baumannii and other OXA β-lactamase-overexpressing Enterobacteriaceae, while having no impact on the normal flora.
34872351	13	62	theme	antibiotics	2153:2163	arg1	option					2188:2193	a primary treatment option	2168:2193	a primary treatment option for A. baumannii infections	2168:2221	The β-lactam class of antibiotics is a primary treatment option for A. baumannii infections, and expression of β-lactamases is the most frequent mechanism of resistance in this bacterium.
34872351	13	62	theme	antibiotics	2153:2163	arg1	class					2144:2148	The β-lactam class	2131:2148	The β-lactam class of antibiotics	2131:2163	The β-lactam class of antibiotics is a primary treatment option for A. baumannii infections, and expression of β-lactamases is the most frequent mechanism of resistance in this bacterium.
34872351	11	63	from	impact	1732:1737	arg1	integrity					1782:1790	peptidoglycan integrity	1768:1790	peptidoglycan integrity	1768:1790	Our results highlight the impact of OXA-23 hyperexpression on peptidoglycan integrity and reveal new genetic vulnerabilities, which may represent novel targets for antimicrobial agents specific to MDR A. baumannii and other OXA β-lactamase-overexpressing Enterobacteriaceae, while having no impact on the normal flora.
34872351	9	64	theme	CRISPR	1491:1496	arg1	CRISPRi					1512:1518	CRISPRi	1512:1518	CRISPRi	1512:1518	In addition, we demonstrated a synergistic lethal relationship between OXA-23 overexpression and a CRISPR interference (CRISPRi) knockdown of the essential peptidoglycan synthesis enzyme MurA.
34872351	9	64	theme	CRISPR	1491:1496	arg1	interference					1498:1509	CRISPR interference	1491:1509	a CRISPR interference (CRISPRi) knockdown of the essential peptidoglycan synthesis enzyme MurA	1489:1582	In addition, we demonstrated a synergistic lethal relationship between OXA-23 overexpression and a CRISPR interference (CRISPRi) knockdown of the essential peptidoglycan synthesis enzyme MurA.
34872351	5	65	theme	peptidoglycan	788:800	arg1	analysis					814:821	peptidoglycan composition analysis	788:821	peptidoglycan composition analysis	788:821	Using peptidoglycan composition analysis, we show that overexpressing the OXA-23 β-lactamase in A. baumannii drives significant collateral changes with alterations consistent with increased amidase activity.
34872351	16	66	theme	OXA-23-expressing	2617:2633	arg1	strains					2635:2641	OXA-23-expressing strains	2617:2641	OXA-23-expressing strains	2617:2641	We have identified genes that become selectively essential in OXA-23-expressing strains and confirmed the relationship between altered peptidoglycan and OXA-23 expression by demonstrating that OXA-23 overexpression sensitizes cells to genetic and chemical inhibition of peptidoglycan synthesis.
34872351	1	67	theme	bacterium	273:281	arg1	baumannii					297:305	the multidrug-resistant (MDR) bacterium Acinetobacter baumannii	243:305	the multidrug-resistant (MDR) bacterium Acinetobacter baumannii	243:305	β-Lactamase expression is the major mechanism of resistance to penicillins, cephalosporins, and carbapenems in the multidrug-resistant (MDR) bacterium Acinetobacter baumannii.
34872351	13	68	from	resistance	2289:2298	arg1	bacterium					2308:2316	this bacterium	2303:2316	this bacterium	2303:2316	The β-lactam class of antibiotics is a primary treatment option for A. baumannii infections, and expression of β-lactamases is the most frequent mechanism of resistance in this bacterium.
34872351	2	69	theme	stable	317:322	arg1	expression					335:344	stable high-level expression	317:344	stable high-level expression of at least one β-lactamase	317:372	In fact, stable high-level expression of at least one β-lactamase has been rapidly increasing and reported to occur in up to 98.5% of modern A. baumannii isolates recovered in the clinic.
34872351	16	70	theme	altered	2682:2688	arg1	peptidoglycan					2690:2702	altered peptidoglycan and OXA-23 expression	2682:2724	peptidoglycan	2690:2702	We have identified genes that become selectively essential in OXA-23-expressing strains and confirmed the relationship between altered peptidoglycan and OXA-23 expression by demonstrating that OXA-23 overexpression sensitizes cells to genetic and chemical inhibition of peptidoglycan synthesis.
34872351	5	71	theme	consistent	946:955	arg1	alterations					934:944	alterations	934:944	alterations consistent with increased amidase activity	934:987	Using peptidoglycan composition analysis, we show that overexpressing the OXA-23 β-lactamase in A. baumannii drives significant collateral changes with alterations consistent with increased amidase activity.
34872351	13	72	from	mechanism	2276:2284	arg1	bacterium					2308:2316	this bacterium	2303:2316	this bacterium	2303:2316	The β-lactam class of antibiotics is a primary treatment option for A. baumannii infections, and expression of β-lactamases is the most frequent mechanism of resistance in this bacterium.
34872351	11	73	theme	genetic	1807:1813	arg1	vulnerabilities					1815:1829	new genetic vulnerabilities	1803:1829	new genetic vulnerabilities	1803:1829	Our results highlight the impact of OXA-23 hyperexpression on peptidoglycan integrity and reveal new genetic vulnerabilities, which may represent novel targets for antimicrobial agents specific to MDR A. baumannii and other OXA β-lactamase-overexpressing Enterobacteriaceae, while having no impact on the normal flora.
34872351	5	74	theme	collateral	910:919	arg1	changes					921:927	significant collateral changes	898:927	significant collateral changes	898:927	Using peptidoglycan composition analysis, we show that overexpressing the OXA-23 β-lactamase in A. baumannii drives significant collateral changes with alterations consistent with increased amidase activity.
34872351	14	75	theme	multidrug-resistant	2346:2364	arg1	strains					2379:2385	multidrug-resistant A. baumannii strains	2346:2385	multidrug-resistant A. baumannii strains	2346:2385	New approaches to treating multidrug-resistant A. baumannii strains are needed.
34872351	9	76	theme	essential	1538:1546	arg1	MurA					1579:1582	the essential peptidoglycan synthesis enzyme MurA	1534:1582	the essential peptidoglycan synthesis enzyme MurA	1534:1582	In addition, we demonstrated a synergistic lethal relationship between OXA-23 overexpression and a CRISPR interference (CRISPRi) knockdown of the essential peptidoglycan synthesis enzyme MurA.
34872351	1	77	from	baumannii	297:305	arg1	expression					144:153	β-Lactamase expression	132:153	β-Lactamase expression	132:153	β-Lactamase expression is the major mechanism of resistance to penicillins, cephalosporins, and carbapenems in the multidrug-resistant (MDR) bacterium Acinetobacter baumannii.
34872351	1	77	from	baumannii	297:305	arg1	mechanism					168:176	the major mechanism	158:176	the major mechanism of resistance to penicillins, cephalosporins, and carbapenems in the multidrug-resistant (MDR) bacterium Acinetobacter baumannii	158:305	β-Lactamase expression is the major mechanism of resistance to penicillins, cephalosporins, and carbapenems in the multidrug-resistant (MDR) bacterium Acinetobacter baumannii.
34872351	3	78	theme	A.	559:560	arg1	chromosome					572:581	the A. baumannii chromosome	555:581	the A. baumannii chromosome	555:581	Moreover, the OXA-51 β-lactamase is universally present in the A. baumannii chromosome, suggesting it may have a cellular function beyond antibiotic resistance.
34872351	9	79	theme	synthesis	1562:1570	arg1	MurA					1579:1582	the essential peptidoglycan synthesis enzyme MurA	1534:1582	the essential peptidoglycan synthesis enzyme MurA	1534:1582	In addition, we demonstrated a synergistic lethal relationship between OXA-23 overexpression and a CRISPR interference (CRISPRi) knockdown of the essential peptidoglycan synthesis enzyme MurA.
34872351	14	80	theme	New	2319:2321	arg1	approaches					2323:2332	New approaches	2319:2332	New approaches to treating multidrug-resistant A. baumannii strains	2319:2385	New approaches to treating multidrug-resistant A. baumannii strains are needed.
34872351	11	81	theme	OXA	1930:1932	arg1	Enterobacteriaceae					1961:1978	other OXA β-lactamase-overexpressing Enterobacteriaceae	1924:1978	other OXA β-lactamase-overexpressing Enterobacteriaceae	1924:1978	Our results highlight the impact of OXA-23 hyperexpression on peptidoglycan integrity and reveal new genetic vulnerabilities, which may represent novel targets for antimicrobial agents specific to MDR A. baumannii and other OXA β-lactamase-overexpressing Enterobacteriaceae, while having no impact on the normal flora.
34872351	9	82	theme	MurA	1579:1582	arg1	knockdown					1521:1529	a CRISPR interference (CRISPRi) knockdown	1489:1529	a CRISPR interference (CRISPRi) knockdown of the essential peptidoglycan synthesis enzyme MurA	1489:1582	In addition, we demonstrated a synergistic lethal relationship between OXA-23 overexpression and a CRISPR interference (CRISPRi) knockdown of the essential peptidoglycan synthesis enzyme MurA.
34872351	9	82	theme	MurA	1579:1582	arg1	overexpression					1470:1483	OXA-23 overexpression	1463:1483	OXA-23 overexpression	1463:1483	In addition, we demonstrated a synergistic lethal relationship between OXA-23 overexpression and a CRISPR interference (CRISPRi) knockdown of the essential peptidoglycan synthesis enzyme MurA.
34872351	12	83	theme	hospital	2098:2105	arg1	settings					2121:2128	both hospital and community settings	2093:2128	settings	2121:2128	IMPORTANCE Acinetobacter baumannii has become a serious pathogen in both hospital and community settings.
34872351	17	84	theme	new	2900:2902	arg1	targets					2918:2924	new antimicrobial targets	2900:2924	new antimicrobial targets	2900:2924	This work paves the way for the identification of new antimicrobial targets, where inhibitors would selectively kill β-lactamase-expressing strains.
34872351	8	85	theme	inactive	1345:1352	arg1	β-lactamase					1361:1371	a catalytically inactive OXA-23 β-lactamase	1329:1371	a catalytically inactive OXA-23 β-lactamase	1329:1371	The identified genes remained conditionally essential even when a catalytically inactive OXA-23 β-lactamase was overexpressed.
34872351	7	86	theme	small	1098:1102	arg1	screen					1104:1109	a small screen	1096:1109	a small screen of random transposon insertions	1096:1141	As proof of principle, a small screen of random transposon insertions revealed three genes, where mutations resulted in a greater than 19-fold loss of viability when OXA-23 was overexpressed.
34872351	7	87	theme	principle	1085:1093	arg1	proof					1076:1080	proof	1076:1080	proof of principle	1076:1093	As proof of principle, a small screen of random transposon insertions revealed three genes, where mutations resulted in a greater than 19-fold loss of viability when OXA-23 was overexpressed.
34872351	5	88	theme	increased	962:970	arg1	activity					980:987	increased amidase activity	962:987	increased amidase activity	962:987	Using peptidoglycan composition analysis, we show that overexpressing the OXA-23 β-lactamase in A. baumannii drives significant collateral changes with alterations consistent with increased amidase activity.
34872351	12	89	theme	community	2111:2119	arg1	settings					2121:2128	both hospital and community settings	2093:2128	settings	2121:2128	IMPORTANCE Acinetobacter baumannii has become a serious pathogen in both hospital and community settings.
34872351	7	90	theme	transposon	1121:1130	arg1	insertions					1132:1141	random transposon insertions	1114:1141	random transposon insertions	1114:1141	As proof of principle, a small screen of random transposon insertions revealed three genes, where mutations resulted in a greater than 19-fold loss of viability when OXA-23 was overexpressed.
34872351	3	91	from	chromosome	572:581	arg1	β-lactamase					517:527	the OXA-51 β-lactamase	506:527	the OXA-51 β-lactamase	506:527	Moreover, the OXA-51 β-lactamase is universally present in the A. baumannii chromosome, suggesting it may have a cellular function beyond antibiotic resistance.
34872351	3	91	from	chromosome	572:581	arg1	present					544:550	present	544:550	present	544:550	Moreover, the OXA-51 β-lactamase is universally present in the A. baumannii chromosome, suggesting it may have a cellular function beyond antibiotic resistance.
34872351	13	92	theme	frequent	2267:2274	arg1	expression					2228:2237	expression	2228:2237	expression of β-lactamases	2228:2253	The β-lactam class of antibiotics is a primary treatment option for A. baumannii infections, and expression of β-lactamases is the most frequent mechanism of resistance in this bacterium.
34872351	13	92	theme	frequent	2267:2274	arg1	mechanism					2276:2284	the most frequent mechanism	2258:2284	the most frequent mechanism of resistance in this bacterium	2258:2316	The β-lactam class of antibiotics is a primary treatment option for A. baumannii infections, and expression of β-lactamases is the most frequent mechanism of resistance in this bacterium.
34872351	13	93	from	option	2188:2193	arg1	bacterium					2308:2316	this bacterium	2303:2316	this bacterium	2303:2316	The β-lactam class of antibiotics is a primary treatment option for A. baumannii infections, and expression of β-lactamases is the most frequent mechanism of resistance in this bacterium.
34872351	3	94	dep	A.	559:560	arg1	baumannii					562:570	baumannii	562:570	baumannii	562:570	Moreover, the OXA-51 β-lactamase is universally present in the A. baumannii chromosome, suggesting it may have a cellular function beyond antibiotic resistance.
34872351	12	95	from	pathogen	2081:2088	arg1	settings					2121:2128	both hospital and community settings	2093:2128	settings	2121:2128	IMPORTANCE Acinetobacter baumannii has become a serious pathogen in both hospital and community settings.
34872351	16	96	theme	peptidoglycan	2825:2837	arg1	synthesis					2839:2847	peptidoglycan synthesis	2825:2847	peptidoglycan synthesis	2825:2847	We have identified genes that become selectively essential in OXA-23-expressing strains and confirmed the relationship between altered peptidoglycan and OXA-23 expression by demonstrating that OXA-23 overexpression sensitizes cells to genetic and chemical inhibition of peptidoglycan synthesis.
34872351	15	97	theme	significant	2481:2491	arg1	changes					2504:2510	significant collateral changes	2481:2510	significant collateral changes	2481:2510	In this study, we demonstrate that overexpressing the OXA-23 β-lactamase leads to significant collateral changes, where peptidoglycan structure is altered.
34872351	15	98	theme	collateral	2493:2502	arg1	changes					2504:2510	significant collateral changes	2481:2510	significant collateral changes	2481:2510	In this study, we demonstrate that overexpressing the OXA-23 β-lactamase leads to significant collateral changes, where peptidoglycan structure is altered.
34872351	0	99	theme	New	103:105	arg1	Vulnerabilities					115:129	New Genetic Vulnerabilities	103:129	New Genetic Vulnerabilities	103:129	OXA-23 β-Lactamase Overexpression in Acinetobacter baumannii Drives Physiological Changes Resulting in New Genetic Vulnerabilities.
34872351	1	100	theme	resistance	181:190	arg1	expression					144:153	β-Lactamase expression	132:153	β-Lactamase expression	132:153	β-Lactamase expression is the major mechanism of resistance to penicillins, cephalosporins, and carbapenems in the multidrug-resistant (MDR) bacterium Acinetobacter baumannii.
34872351	1	100	theme	resistance	181:190	arg1	mechanism					168:176	the major mechanism	158:176	the major mechanism of resistance to penicillins, cephalosporins, and carbapenems in the multidrug-resistant (MDR) bacterium Acinetobacter baumannii	158:305	β-Lactamase expression is the major mechanism of resistance to penicillins, cephalosporins, and carbapenems in the multidrug-resistant (MDR) bacterium Acinetobacter baumannii.
34872351	4	101	theme	β-lactamase	703:713	arg1	overexpression					715:728	OXA β-lactamase overexpression	699:728	OXA β-lactamase overexpression on A. baumannii physiology	699:755	However, the consequences associated with OXA β-lactamase overexpression on A. baumannii physiology are not well understood.
34872351	13	102	theme	β-lactamases	2242:2253	arg1	expression					2228:2237	expression	2228:2237	expression of β-lactamases	2228:2253	The β-lactam class of antibiotics is a primary treatment option for A. baumannii infections, and expression of β-lactamases is the most frequent mechanism of resistance in this bacterium.
34872351	13	102	theme	β-lactamases	2242:2253	arg1	mechanism					2276:2284	the most frequent mechanism	2258:2284	the most frequent mechanism of resistance in this bacterium	2258:2316	The β-lactam class of antibiotics is a primary treatment option for A. baumannii infections, and expression of β-lactamases is the most frequent mechanism of resistance in this bacterium.
34872351	6	103	theme	cellular	1047:1054	arg1	vulnerabilities					1056:1070	new cellular vulnerabilities	1043:1070	new cellular vulnerabilities	1043:1070	Consequently, we predicted that these changes create new cellular vulnerabilities.
34872351	11	104	theme	A.	1907:1908	arg1	MDR					1903:1905	MDR A. baumannii	1903:1918	MDR A. baumannii	1903:1918	Our results highlight the impact of OXA-23 hyperexpression on peptidoglycan integrity and reveal new genetic vulnerabilities, which may represent novel targets for antimicrobial agents specific to MDR A. baumannii and other OXA β-lactamase-overexpressing Enterobacteriaceae, while having no impact on the normal flora.
34872351	13	105	theme	baumannii	2202:2210	arg1	infections					2212:2221	A. baumannii infections	2199:2221	A. baumannii infections	2199:2221	The β-lactam class of antibiotics is a primary treatment option for A. baumannii infections, and expression of β-lactamases is the most frequent mechanism of resistance in this bacterium.
34872351	11	106	from	impact	1997:2002	arg1	flora					2018:2022	the normal flora	2007:2022	the normal flora	2007:2022	Our results highlight the impact of OXA-23 hyperexpression on peptidoglycan integrity and reveal new genetic vulnerabilities, which may represent novel targets for antimicrobial agents specific to MDR A. baumannii and other OXA β-lactamase-overexpressing Enterobacteriaceae, while having no impact on the normal flora.
34872351	0	107	theme	β-Lactamase	7:17	arg1	Overexpression					19:32	OXA-23 β-Lactamase Overexpression	0:32	OXA-23 β-Lactamase Overexpression in Acinetobacter baumannii	0:59	OXA-23 β-Lactamase Overexpression in Acinetobacter baumannii Drives Physiological Changes Resulting in New Genetic Vulnerabilities.
34872351	2	108	theme	A.	449:450	arg1	isolates					462:469	modern A. baumannii isolates	442:469	modern A. baumannii isolates recovered in the clinic	442:493	In fact, stable high-level expression of at least one β-lactamase has been rapidly increasing and reported to occur in up to 98.5% of modern A. baumannii isolates recovered in the clinic.
34872351	7	109	dep	19-fold	1208:1214	arg1	than					1203:1206	than	1203:1206	than	1203:1206	As proof of principle, a small screen of random transposon insertions revealed three genes, where mutations resulted in a greater than 19-fold loss of viability when OXA-23 was overexpressed.
34872351	7	109	dep	19-fold	1208:1214	arg1	greater					1195:1201	greater	1195:1201	greater	1195:1201	As proof of principle, a small screen of random transposon insertions revealed three genes, where mutations resulted in a greater than 19-fold loss of viability when OXA-23 was overexpressed.
34872351	15	110	theme	OXA-23	2453:2458	arg1	β-lactamase					2460:2470	the OXA-23 β-lactamase	2449:2470	the OXA-23 β-lactamase	2449:2470	In this study, we demonstrate that overexpressing the OXA-23 β-lactamase leads to significant collateral changes, where peptidoglycan structure is altered.
34872351	4	111	dep	A.	733:734	arg1	baumannii					736:744	baumannii	736:744	baumannii	736:744	However, the consequences associated with OXA β-lactamase overexpression on A. baumannii physiology are not well understood.
34872351	10	112	theme	OXA-23	1591:1596	arg1	overexpression					1598:1611	OXA-23 overexpression	1591:1611	OXA-23 overexpression	1591:1611	Last, OXA-23 overexpression sensitized cells to two inhibitors of peptidoglycan synthesis, d-cycloserine and fosfomycin.
34872351	9	113	theme	lethal	1435:1440	arg1	relationship					1442:1453	a synergistic lethal relationship	1421:1453	a synergistic lethal relationship between OXA-23 overexpression and a CRISPR interference (CRISPRi) knockdown of the essential peptidoglycan synthesis enzyme MurA	1421:1582	In addition, we demonstrated a synergistic lethal relationship between OXA-23 overexpression and a CRISPR interference (CRISPRi) knockdown of the essential peptidoglycan synthesis enzyme MurA.
34872351	12	114	theme	Acinetobacter	2036:2048	arg1	baumannii					2050:2058	IMPORTANCE Acinetobacter baumannii	2025:2058	IMPORTANCE Acinetobacter baumannii	2025:2058	IMPORTANCE Acinetobacter baumannii has become a serious pathogen in both hospital and community settings.
34872351	16	115	theme	OXA-23	2708:2713	arg1	expression					2715:2724	altered peptidoglycan and OXA-23 expression	2682:2724	expression	2715:2724	We have identified genes that become selectively essential in OXA-23-expressing strains and confirmed the relationship between altered peptidoglycan and OXA-23 expression by demonstrating that OXA-23 overexpression sensitizes cells to genetic and chemical inhibition of peptidoglycan synthesis.
34872351	13	116	from	bacterium	2308:2316	arg1	expression					2228:2237	expression	2228:2237	expression of β-lactamases	2228:2253	The β-lactam class of antibiotics is a primary treatment option for A. baumannii infections, and expression of β-lactamases is the most frequent mechanism of resistance in this bacterium.
34872351	13	116	from	bacterium	2308:2316	arg1	option					2188:2193	a primary treatment option	2168:2193	a primary treatment option for A. baumannii infections	2168:2221	The β-lactam class of antibiotics is a primary treatment option for A. baumannii infections, and expression of β-lactamases is the most frequent mechanism of resistance in this bacterium.
34872351	13	116	from	bacterium	2308:2316	arg1	mechanism					2276:2284	the most frequent mechanism	2258:2284	the most frequent mechanism of resistance in this bacterium	2258:2316	The β-lactam class of antibiotics is a primary treatment option for A. baumannii infections, and expression of β-lactamases is the most frequent mechanism of resistance in this bacterium.
34872351	13	116	from	bacterium	2308:2316	arg1	class					2144:2148	The β-lactam class	2131:2148	The β-lactam class of antibiotics	2131:2163	The β-lactam class of antibiotics is a primary treatment option for A. baumannii infections, and expression of β-lactamases is the most frequent mechanism of resistance in this bacterium.
34872351	3	117	theme	antibiotic	634:643	arg1	resistance					645:654	antibiotic resistance	634:654	antibiotic resistance	634:654	Moreover, the OXA-51 β-lactamase is universally present in the A. baumannii chromosome, suggesting it may have a cellular function beyond antibiotic resistance.
34872351	1	118	theme	MDR	268:270	arg1	baumannii					297:305	the multidrug-resistant (MDR) bacterium Acinetobacter baumannii	243:305	the multidrug-resistant (MDR) bacterium Acinetobacter baumannii	243:305	β-Lactamase expression is the major mechanism of resistance to penicillins, cephalosporins, and carbapenems in the multidrug-resistant (MDR) bacterium Acinetobacter baumannii.
34872351	13	119	theme	primary	2170:2176	arg1	option					2188:2193	a primary treatment option	2168:2193	a primary treatment option for A. baumannii infections	2168:2221	The β-lactam class of antibiotics is a primary treatment option for A. baumannii infections, and expression of β-lactamases is the most frequent mechanism of resistance in this bacterium.
34872351	13	119	theme	primary	2170:2176	arg1	class					2144:2148	The β-lactam class	2131:2148	The β-lactam class of antibiotics	2131:2163	The β-lactam class of antibiotics is a primary treatment option for A. baumannii infections, and expression of β-lactamases is the most frequent mechanism of resistance in this bacterium.
34872351	0	120	theme	Physiological	68:80	arg1	Changes					82:88	Physiological Changes	68:88	Physiological Changes Resulting in New Genetic Vulnerabilities	68:129	OXA-23 β-Lactamase Overexpression in Acinetobacter baumannii Drives Physiological Changes Resulting in New Genetic Vulnerabilities.
34872351	2	121	theme	isolates	462:469	arg1	isolates					462:469	modern A. baumannii isolates	442:469	modern A. baumannii isolates recovered in the clinic	442:493	In fact, stable high-level expression of at least one β-lactamase has been rapidly increasing and reported to occur in up to 98.5% of modern A. baumannii isolates recovered in the clinic.
34872351	2	121	theme	isolates	462:469	arg1	%					437:437	up to 98.5%	427:437	up to 98.5% of modern A. baumannii isolates recovered in the clinic	427:493	In fact, stable high-level expression of at least one β-lactamase has been rapidly increasing and reported to occur in up to 98.5% of modern A. baumannii isolates recovered in the clinic.
34872351	2	122	dep	98.5	433:436	arg1	to					430:431	to	430:431	to	430:431	In fact, stable high-level expression of at least one β-lactamase has been rapidly increasing and reported to occur in up to 98.5% of modern A. baumannii isolates recovered in the clinic.
34872351	2	123	theme	β-lactamase	362:372	arg1	expression					335:344	stable high-level expression	317:344	stable high-level expression of at least one β-lactamase	317:372	In fact, stable high-level expression of at least one β-lactamase has been rapidly increasing and reported to occur in up to 98.5% of modern A. baumannii isolates recovered in the clinic.
34872351	11	124	theme	normal	2011:2016	arg1	flora					2018:2022	the normal flora	2007:2022	the normal flora	2007:2022	Our results highlight the impact of OXA-23 hyperexpression on peptidoglycan integrity and reveal new genetic vulnerabilities, which may represent novel targets for antimicrobial agents specific to MDR A. baumannii and other OXA β-lactamase-overexpressing Enterobacteriaceae, while having no impact on the normal flora.
34872351	10	125	theme	peptidoglycan	1651:1663	arg1	synthesis					1665:1673	peptidoglycan synthesis	1651:1673	peptidoglycan synthesis	1651:1673	Last, OXA-23 overexpression sensitized cells to two inhibitors of peptidoglycan synthesis, d-cycloserine and fosfomycin.
34872351	11	126	theme	peptidoglycan	1768:1780	arg1	integrity					1782:1790	peptidoglycan integrity	1768:1790	peptidoglycan integrity	1768:1790	Our results highlight the impact of OXA-23 hyperexpression on peptidoglycan integrity and reveal new genetic vulnerabilities, which may represent novel targets for antimicrobial agents specific to MDR A. baumannii and other OXA β-lactamase-overexpressing Enterobacteriaceae, while having no impact on the normal flora.
34872351	7	127	theme	viability	1224:1232	arg1	loss					1216:1219	a greater than 19-fold loss	1193:1219	a greater than 19-fold loss of viability	1193:1232	As proof of principle, a small screen of random transposon insertions revealed three genes, where mutations resulted in a greater than 19-fold loss of viability when OXA-23 was overexpressed.
34872351	4	128	from	overexpression	715:728	arg1	physiology					746:755	A. baumannii physiology	733:755	A. baumannii physiology	733:755	However, the consequences associated with OXA β-lactamase overexpression on A. baumannii physiology are not well understood.
34872351	9	129	theme	interference	1498:1509	arg1	knockdown					1521:1529	a CRISPR interference (CRISPRi) knockdown	1489:1529	a CRISPR interference (CRISPRi) knockdown of the essential peptidoglycan synthesis enzyme MurA	1489:1582	In addition, we demonstrated a synergistic lethal relationship between OXA-23 overexpression and a CRISPR interference (CRISPRi) knockdown of the essential peptidoglycan synthesis enzyme MurA.
34209998	10	0	theme	antibacterial	1456:1468	arg1	preparations					1470:1481	new antibacterial preparations	1452:1481	new antibacterial preparations	1452:1481	In addition, our attempt to formulate the phage into hydroxyethyl cellulose (HEC) cream may provide new antibacterial preparations for human infections.
34209998	6	1	theme	family	875:880	arg1	Siphoviridae					882:893	the family Siphoviridae	871:893	the family Siphoviridae with an icosahedral head and a non-contractile tail	871:945	Morphological analysis revealed that TCUCAP1 belongs to the family Siphoviridae with an icosahedral head and a non-contractile tail.
34209998	7	2	theme	reading	1059:1065	arg1	ORFs					1075:1078	ORFs	1075:1078	ORFs	1075:1078	Genome analysis found that TCUCAP1 is composed of 29,547 bp with a G+C content of 53.83% and 56 predicted open reading frames (ORFs).
34209998	7	2	theme	reading	1059:1065	arg1	frames					1067:1072	open reading frames	1054:1072	56 predicted open reading frames (ORFs)	1041:1079	Genome analysis found that TCUCAP1 is composed of 29,547 bp with a G+C content of 53.83% and 56 predicted open reading frames (ORFs).
34209998	7	3	theme	Genome	948:953	arg1	analysis					955:962	Genome analysis	948:962	Genome analysis	948:962	Genome analysis found that TCUCAP1 is composed of 29,547 bp with a G+C content of 53.83% and 56 predicted open reading frames (ORFs).
34209998	3	4	theme	acne	467:470	arg1	lesions					472:478	acne lesions	467:478	acne lesions	467:478	While antibiotics have been used for several decades to treat acne lesions, antibiotic resistance is a growing crisis; thus, finding a new therapeutic target is urgently needed.
34209998	1	5	theme	common	231:236	arg1	vulgaris					132:139	Acne vulgaris	127:139	Acne vulgaris	127:139	Acne vulgaris, which is mostly associated with the colonization of Cutibacterium acnes (C. acnes), is a common skin inflammatory disease in teenagers.
34209998	1	5	theme	common	231:236	arg1	disease					256:262	a common skin inflammatory disease	229:262	a common skin inflammatory disease in teenagers	229:275	Acne vulgaris, which is mostly associated with the colonization of Cutibacterium acnes (C. acnes), is a common skin inflammatory disease in teenagers.
34209998	10	6	theme	new	1452:1454	arg1	preparations					1470:1481	new antibacterial preparations	1452:1481	new antibacterial preparations	1452:1481	In addition, our attempt to formulate the phage into hydroxyethyl cellulose (HEC) cream may provide new antibacterial preparations for human infections.
34209998	8	7	theme	host	1138:1141	arg1	lysis					1143:1147	host lysis	1138:1147	host lysis	1138:1147	The ORFs were associated with phage structure, packing, host lysis, DNA metabolism, and additional functions.
34209998	8	8	theme	DNA	1150:1152	arg1	metabolism					1154:1163	DNA metabolism	1150:1163	DNA metabolism	1150:1163	The ORFs were associated with phage structure, packing, host lysis, DNA metabolism, and additional functions.
34209998	1	9	theme	skin	238:241	arg1	vulgaris					132:139	Acne vulgaris	127:139	Acne vulgaris	127:139	Acne vulgaris, which is mostly associated with the colonization of Cutibacterium acnes (C. acnes), is a common skin inflammatory disease in teenagers.
34209998	1	9	theme	skin	238:241	arg1	disease					256:262	a common skin inflammatory disease	229:262	a common skin inflammatory disease in teenagers	229:275	Acne vulgaris, which is mostly associated with the colonization of Cutibacterium acnes (C. acnes), is a common skin inflammatory disease in teenagers.
34209998	7	10	theme	predicted	1044:1052	arg1	ORFs					1075:1078	ORFs	1075:1078	ORFs	1075:1078	Genome analysis found that TCUCAP1 is composed of 29,547 bp with a G+C content of 53.83% and 56 predicted open reading frames (ORFs).
34209998	7	10	theme	predicted	1044:1052	arg1	frames					1067:1072	open reading frames	1054:1072	56 predicted open reading frames (ORFs)	1041:1079	Genome analysis found that TCUCAP1 is composed of 29,547 bp with a G+C content of 53.83% and 56 predicted open reading frames (ORFs).
34209998	11	11	theme	several	1551:1557	arg1	features					1559:1566	several features	1551:1566	several features that make it an ideal candidate for the control of C. acnes infections	1551:1637	Our results demonstrate that TCUCAP1 displays several features that make it an ideal candidate for the control of C. acnes infections.
34209998	1	12	theme	inflammatory	243:254	arg1	vulgaris					132:139	Acne vulgaris	127:139	Acne vulgaris	127:139	Acne vulgaris, which is mostly associated with the colonization of Cutibacterium acnes (C. acnes), is a common skin inflammatory disease in teenagers.
34209998	1	12	theme	inflammatory	243:254	arg1	disease					256:262	a common skin inflammatory disease	229:262	a common skin inflammatory disease in teenagers	229:275	Acne vulgaris, which is mostly associated with the colonization of Cutibacterium acnes (C. acnes), is a common skin inflammatory disease in teenagers.
34209998	7	13	theme	open	1054:1057	arg1	ORFs					1075:1078	ORFs	1075:1078	ORFs	1075:1078	Genome analysis found that TCUCAP1 is composed of 29,547 bp with a G+C content of 53.83% and 56 predicted open reading frames (ORFs).
34209998	7	13	theme	open	1054:1057	arg1	frames					1067:1072	open reading frames	1054:1072	56 predicted open reading frames (ORFs)	1041:1079	Genome analysis found that TCUCAP1 is composed of 29,547 bp with a G+C content of 53.83% and 56 predicted open reading frames (ORFs).
34209998	3	14	theme	growing	508:514	arg1	resistance					492:501	antibiotic resistance	481:501	antibiotic resistance	481:501	While antibiotics have been used for several decades to treat acne lesions, antibiotic resistance is a growing crisis; thus, finding a new therapeutic target is urgently needed.
34209998	3	14	theme	growing	508:514	arg1	crisis					516:521	a growing crisis	506:521	a growing crisis	506:521	While antibiotics have been used for several decades to treat acne lesions, antibiotic resistance is a growing crisis; thus, finding a new therapeutic target is urgently needed.
34209998	4	15	theme	bacterial	678:686	arg1	infections					688:697	multi-drug-resistant bacterial infections	657:697	multi-drug-resistant bacterial infections	657:697	Studies have shown that phage therapy may be one alternative for treating multi-drug-resistant bacterial infections.
34209998	11	16	theme	ideal	1584:1588	arg1	candidate					1590:1598	an ideal candidate	1581:1598	an ideal candidate for the control of C. acnes infections	1581:1637	Our results demonstrate that TCUCAP1 displays several features that make it an ideal candidate for the control of C. acnes infections.
34209998	4	17	theme	multi-drug-resistant	657:676	arg1	infections					688:697	multi-drug-resistant bacterial infections	657:697	multi-drug-resistant bacterial infections	657:697	Studies have shown that phage therapy may be one alternative for treating multi-drug-resistant bacterial infections.
34209998	5	18	dep	C.	749:750	arg1	acnes					752:756	acnes	752:756	acnes	752:756	In the present study, we successfully isolated a C. acnes phage named TCUCAP1 from the skin of healthy volunteers.
34209998	4	19	theme	phage	607:611	arg1	therapy					613:619	phage therapy	607:619	phage therapy	607:619	Studies have shown that phage therapy may be one alternative for treating multi-drug-resistant bacterial infections.
34209998	5	20	theme	present	707:713	arg1	study					715:719	the present study	703:719	the present study	703:719	In the present study, we successfully isolated a C. acnes phage named TCUCAP1 from the skin of healthy volunteers.
34209998	10	21	theme	hydroxyethyl	1405:1416	arg1	cream					1434:1438	hydroxyethyl cellulose (HEC) cream	1405:1438	hydroxyethyl cellulose (HEC) cream	1405:1438	In addition, our attempt to formulate the phage into hydroxyethyl cellulose (HEC) cream may provide new antibacterial preparations for human infections.
34209998	10	21	theme	hydroxyethyl	1405:1416	arg1	HEC					1429:1431	HEC	1429:1431	HEC	1429:1431	In addition, our attempt to formulate the phage into hydroxyethyl cellulose (HEC) cream may provide new antibacterial preparations for human infections.
34209998	8	22	theme	phage	1112:1116	arg1	structure					1118:1126	phage structure	1112:1126	phage structure	1112:1126	The ORFs were associated with phage structure, packing, host lysis, DNA metabolism, and additional functions.
34209998	2	23	theme	past	296:299	arg1	years					305:309	the past few years	292:309	the past few years	292:309	However, over the past few years, the disease has extended beyond childhood to chronically infect approximately 40% of adults.
34209998	7	24	with	bp	1005:1006	arg1	content					1019:1025	a G+C content	1013:1025	a G+C content of 53.83%	1013:1035	Genome analysis found that TCUCAP1 is composed of 29,547 bp with a G+C content of 53.83% and 56 predicted open reading frames (ORFs).
34209998	7	24	with	bp	1005:1006	arg1	ORFs					1075:1078	ORFs	1075:1078	ORFs	1075:1078	Genome analysis found that TCUCAP1 is composed of 29,547 bp with a G+C content of 53.83% and 56 predicted open reading frames (ORFs).
34209998	7	24	with	bp	1005:1006	arg1	frames					1067:1072	open reading frames	1054:1072	56 predicted open reading frames (ORFs)	1041:1079	Genome analysis found that TCUCAP1 is composed of 29,547 bp with a G+C content of 53.83% and 56 predicted open reading frames (ORFs).
34209998	0	25	theme	Therapeutic	0:10	arg1	Effect					12:17	Therapeutic Effect	0:17	Therapeutic Effect of a Newly Isolated Lytic Bacteriophage against Multi-Drug-Resistant Cutibacterium	0:100	Therapeutic Effect of a Newly Isolated Lytic Bacteriophage against Multi-Drug-Resistant Cutibacterium acnes Infection in Mice.
34209998	7	26	theme	%	1035:1035	arg1	content					1019:1025	a G+C content	1013:1025	a G+C content of 53.83%	1013:1035	Genome analysis found that TCUCAP1 is composed of 29,547 bp with a G+C content of 53.83% and 56 predicted open reading frames (ORFs).
34209998	7	26	theme	%	1035:1035	arg1	ORFs					1075:1078	ORFs	1075:1078	ORFs	1075:1078	Genome analysis found that TCUCAP1 is composed of 29,547 bp with a G+C content of 53.83% and 56 predicted open reading frames (ORFs).
34209998	7	26	theme	%	1035:1035	arg1	frames					1067:1072	open reading frames	1054:1072	56 predicted open reading frames (ORFs)	1041:1079	Genome analysis found that TCUCAP1 is composed of 29,547 bp with a G+C content of 53.83% and 56 predicted open reading frames (ORFs).
34209998	11	27	theme	infections	1628:1637	arg1	control					1608:1614	the control	1604:1614	the control of C. acnes infections	1604:1637	Our results demonstrate that TCUCAP1 displays several features that make it an ideal candidate for the control of C. acnes infections.
34209998	6	28	theme	non-contractile	926:940	arg1	tail					942:945	a non-contractile tail	924:945	a non-contractile tail	924:945	Morphological analysis revealed that TCUCAP1 belongs to the family Siphoviridae with an icosahedral head and a non-contractile tail.
34209998	1	29	theme	acnes	208:212	arg1	colonization					178:189	the colonization	174:189	the colonization of Cutibacterium acnes (C. acnes)	174:223	Acne vulgaris, which is mostly associated with the colonization of Cutibacterium acnes (C. acnes), is a common skin inflammatory disease in teenagers.
34209998	2	30	theme	adults	397:402	arg1	adults					397:402	adults	397:402	adults	397:402	However, over the past few years, the disease has extended beyond childhood to chronically infect approximately 40% of adults.
34209998	2	30	theme	adults	397:402	arg1	%					392:392	approximately 40%	376:392	approximately 40% of adults	376:402	However, over the past few years, the disease has extended beyond childhood to chronically infect approximately 40% of adults.
34209998	9	31	theme	C.-acnes-induced	1257:1272	arg1	inflammation					1279:1290	multi-drug-resistant (MDR) C.-acnes-induced skin inflammation	1230:1290	multi-drug-resistant (MDR) C.-acnes-induced skin inflammation	1230:1290	Phage treatments applied to mice with multi-drug-resistant (MDR) C.-acnes-induced skin inflammation resulted in a significant decrease in inflammatory lesions.
34209998	1	32	from	disease	256:262	arg1	teenagers					267:275	teenagers	267:275	teenagers	267:275	Acne vulgaris, which is mostly associated with the colonization of Cutibacterium acnes (C. acnes), is a common skin inflammatory disease in teenagers.
34209998	11	33	theme	acnes	1622:1626	arg1	infections					1628:1637	C. acnes infections	1619:1637	C. acnes infections	1619:1637	Our results demonstrate that TCUCAP1 displays several features that make it an ideal candidate for the control of C. acnes infections.
34209998	3	34	used	used	433:436	arg2	antibiotics					411:421	antibiotics	411:421	antibiotics	411:421	While antibiotics have been used for several decades to treat acne lesions, antibiotic resistance is a growing crisis; thus, finding a new therapeutic target is urgently needed.
34209998	0	35	theme	Isolated	30:37	arg1	Bacteriophage					45:57	a Newly Isolated Lytic Bacteriophage	22:57	a Newly Isolated Lytic Bacteriophage	22:57	Therapeutic Effect of a Newly Isolated Lytic Bacteriophage against Multi-Drug-Resistant Cutibacterium acnes Infection in Mice.
34209998	9	36	theme	skin	1274:1277	arg1	inflammation					1279:1290	multi-drug-resistant (MDR) C.-acnes-induced skin inflammation	1230:1290	multi-drug-resistant (MDR) C.-acnes-induced skin inflammation	1230:1290	Phage treatments applied to mice with multi-drug-resistant (MDR) C.-acnes-induced skin inflammation resulted in a significant decrease in inflammatory lesions.
34209998	10	37	theme	cellulose	1418:1426	arg1	cream					1434:1438	hydroxyethyl cellulose (HEC) cream	1405:1438	hydroxyethyl cellulose (HEC) cream	1405:1438	In addition, our attempt to formulate the phage into hydroxyethyl cellulose (HEC) cream may provide new antibacterial preparations for human infections.
34209998	10	37	theme	cellulose	1418:1426	arg1	HEC					1429:1431	HEC	1429:1431	HEC	1429:1431	In addition, our attempt to formulate the phage into hydroxyethyl cellulose (HEC) cream may provide new antibacterial preparations for human infections.
34209998	6	38	theme	Morphological	815:827	arg1	analysis					829:836	Morphological analysis	815:836	Morphological analysis	815:836	Morphological analysis revealed that TCUCAP1 belongs to the family Siphoviridae with an icosahedral head and a non-contractile tail.
34209998	3	39	theme	new	540:542	arg1	target					556:561	a new therapeutic target	538:561	finding a new therapeutic target	530:561	While antibiotics have been used for several decades to treat acne lesions, antibiotic resistance is a growing crisis; thus, finding a new therapeutic target is urgently needed.
34209998	5	40	theme	volunteers	803:812	arg1	skin					787:790	the skin	783:790	the skin of healthy volunteers	783:812	In the present study, we successfully isolated a C. acnes phage named TCUCAP1 from the skin of healthy volunteers.
34209998	7	41	theme	G+C	1015:1017	arg1	content					1019:1025	a G+C content	1013:1025	a G+C content of 53.83%	1013:1035	Genome analysis found that TCUCAP1 is composed of 29,547 bp with a G+C content of 53.83% and 56 predicted open reading frames (ORFs).
34209998	3	42	theme	therapeutic	544:554	arg1	target					556:561	a new therapeutic target	538:561	finding a new therapeutic target	530:561	While antibiotics have been used for several decades to treat acne lesions, antibiotic resistance is a growing crisis; thus, finding a new therapeutic target is urgently needed.
34209998	5	43	theme	healthy	795:801	arg1	volunteers					803:812	healthy volunteers	795:812	healthy volunteers	795:812	In the present study, we successfully isolated a C. acnes phage named TCUCAP1 from the skin of healthy volunteers.
34209998	0	44	theme	Bacteriophage	45:57	arg1	Effect					12:17	Therapeutic Effect	0:17	Therapeutic Effect of a Newly Isolated Lytic Bacteriophage against Multi-Drug-Resistant Cutibacterium	0:100	Therapeutic Effect of a Newly Isolated Lytic Bacteriophage against Multi-Drug-Resistant Cutibacterium acnes Infection in Mice.
34209998	6	45	theme	icosahedral	903:913	arg1	head					915:918	an icosahedral head	900:918	an icosahedral head	900:918	Morphological analysis revealed that TCUCAP1 belongs to the family Siphoviridae with an icosahedral head and a non-contractile tail.
34209998	11	46	theme	C.	1619:1620	arg1	infections					1628:1637	C. acnes infections	1619:1637	C. acnes infections	1619:1637	Our results demonstrate that TCUCAP1 displays several features that make it an ideal candidate for the control of C. acnes infections.
34209998	3	47	theme	finding	530:536	arg1	target					556:561	a new therapeutic target	538:561	finding a new therapeutic target	530:561	While antibiotics have been used for several decades to treat acne lesions, antibiotic resistance is a growing crisis; thus, finding a new therapeutic target is urgently needed.
34209998	1	48	theme	Acne	127:130	arg1	vulgaris					132:139	Acne vulgaris	127:139	Acne vulgaris	127:139	Acne vulgaris, which is mostly associated with the colonization of Cutibacterium acnes (C. acnes), is a common skin inflammatory disease in teenagers.
34209998	1	48	theme	Acne	127:130	arg1	disease					256:262	a common skin inflammatory disease	229:262	a common skin inflammatory disease in teenagers	229:275	Acne vulgaris, which is mostly associated with the colonization of Cutibacterium acnes (C. acnes), is a common skin inflammatory disease in teenagers.
34209998	0	49	theme	Lytic	39:43	arg1	Bacteriophage					45:57	a Newly Isolated Lytic Bacteriophage	22:57	a Newly Isolated Lytic Bacteriophage	22:57	Therapeutic Effect of a Newly Isolated Lytic Bacteriophage against Multi-Drug-Resistant Cutibacterium acnes Infection in Mice.
34209998	10	50	theme	human	1487:1491	arg1	infections					1493:1502	human infections	1487:1502	human infections	1487:1502	In addition, our attempt to formulate the phage into hydroxyethyl cellulose (HEC) cream may provide new antibacterial preparations for human infections.
34209998	3	51	theme	several	442:448	arg1	decades					450:456	several decades	442:456	several decades	442:456	While antibiotics have been used for several decades to treat acne lesions, antibiotic resistance is a growing crisis; thus, finding a new therapeutic target is urgently needed.
34209998	9	52	theme	significant	1306:1316	arg1	decrease					1318:1325	a significant decrease	1304:1325	a significant decrease in inflammatory lesions	1304:1349	Phage treatments applied to mice with multi-drug-resistant (MDR) C.-acnes-induced skin inflammation resulted in a significant decrease in inflammatory lesions.
34209998	9	53	from	decrease	1318:1325	arg1	lesions					1343:1349	inflammatory lesions	1330:1349	inflammatory lesions	1330:1349	Phage treatments applied to mice with multi-drug-resistant (MDR) C.-acnes-induced skin inflammation resulted in a significant decrease in inflammatory lesions.
34209998	2	54	theme	few	301:303	arg1	years					305:309	the past few years	292:309	the past few years	292:309	However, over the past few years, the disease has extended beyond childhood to chronically infect approximately 40% of adults.
34209998	9	55	with	mice	1220:1223	arg1	inflammation					1279:1290	multi-drug-resistant (MDR) C.-acnes-induced skin inflammation	1230:1290	multi-drug-resistant (MDR) C.-acnes-induced skin inflammation	1230:1290	Phage treatments applied to mice with multi-drug-resistant (MDR) C.-acnes-induced skin inflammation resulted in a significant decrease in inflammatory lesions.
34209998	6	56	with	Siphoviridae	882:893	arg1	head					915:918	an icosahedral head	900:918	an icosahedral head	900:918	Morphological analysis revealed that TCUCAP1 belongs to the family Siphoviridae with an icosahedral head and a non-contractile tail.
34209998	6	56	with	Siphoviridae	882:893	arg1	tail					942:945	a non-contractile tail	924:945	a non-contractile tail	924:945	Morphological analysis revealed that TCUCAP1 belongs to the family Siphoviridae with an icosahedral head and a non-contractile tail.
34209998	0	57	theme	Multi-Drug-Resistant	67:86	arg1	Cutibacterium					88:100	Multi-Drug-Resistant Cutibacterium	67:100	Multi-Drug-Resistant Cutibacterium	67:100	Therapeutic Effect of a Newly Isolated Lytic Bacteriophage against Multi-Drug-Resistant Cutibacterium acnes Infection in Mice.
34209998	0	58	from	Infection	108:116	arg1	Mice					121:124	Mice	121:124	Mice	121:124	Therapeutic Effect of a Newly Isolated Lytic Bacteriophage against Multi-Drug-Resistant Cutibacterium acnes Infection in Mice.
34209998	1	59	dep	acnes	208:212	arg1	acnes					218:222	C. acnes	215:222	C. acnes	215:222	Acne vulgaris, which is mostly associated with the colonization of Cutibacterium acnes (C. acnes), is a common skin inflammatory disease in teenagers.
34209998	8	60	theme	additional	1170:1179	arg1	functions					1181:1189	additional functions	1170:1189	additional functions	1170:1189	The ORFs were associated with phage structure, packing, host lysis, DNA metabolism, and additional functions.
34209998	5	61	theme	C.	749:750	arg1	phage					758:762	a C. acnes phage	747:762	a C. acnes phage named TCUCAP1 from the skin of healthy volunteers	747:812	In the present study, we successfully isolated a C. acnes phage named TCUCAP1 from the skin of healthy volunteers.
34209998	9	62	theme	Phage	1192:1196	arg1	treatments					1198:1207	Phage treatments	1192:1207	Phage treatments applied to mice with multi-drug-resistant (MDR) C.-acnes-induced skin inflammation	1192:1290	Phage treatments applied to mice with multi-drug-resistant (MDR) C.-acnes-induced skin inflammation resulted in a significant decrease in inflammatory lesions.
34209998	3	63	theme	antibiotic	481:490	arg1	resistance					492:501	antibiotic resistance	481:501	antibiotic resistance	481:501	While antibiotics have been used for several decades to treat acne lesions, antibiotic resistance is a growing crisis; thus, finding a new therapeutic target is urgently needed.
34209998	3	63	theme	antibiotic	481:490	arg1	crisis					516:521	a growing crisis	506:521	a growing crisis	506:521	While antibiotics have been used for several decades to treat acne lesions, antibiotic resistance is a growing crisis; thus, finding a new therapeutic target is urgently needed.
34209998	9	64	theme	inflammatory	1330:1341	arg1	lesions					1343:1349	inflammatory lesions	1330:1349	inflammatory lesions	1330:1349	Phage treatments applied to mice with multi-drug-resistant (MDR) C.-acnes-induced skin inflammation resulted in a significant decrease in inflammatory lesions.
32921469	3	0	theme	%	922:922	arg1	straw					948:952	7.6% chopped untreated wheat straw	919:952	7.6% chopped untreated wheat straw	919:952	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	3	1	theme	mixed	818:822	arg1	TMR					832:834	TMR	832:834	TMR	832:834	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	3	1	theme	mixed	818:822	arg1	ration					824:829	a total mixed ration	810:829	a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS),	810:1046	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	1	2	theme	dairy	235:239	arg1	cattle					241:246	high-producing dairy cattle	220:246	high-producing dairy cattle with low or high starch concentrations	220:285	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	2	3	theme	body	502:505	arg1	weight					507:512	body weight	502:512	body weight	502:512	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	8	4	theme	starch	1726:1731	arg1	levels					1716:1721	low levels	1712:1721	low levels of starch	1712:1731	Diets with NIS were more positively effective when fed with low levels of starch.
32921469	3	5	theme	straw	948:952	arg1	pellets					994:1000	42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets	880:1000	42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets	880:1000	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	2	6	theme	weight	615:620	arg1	n					588:588	n	588:588	n = 4; 878 ± 67 kg	588:605	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	1	7	theme	rumen	401:405	arg1	function					407:414	rumen function	401:414	rumen function	401:414	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	7	8	theme	high-starch	1610:1620	arg1	diets					1622:1626	NIS and high-starch diets	1602:1626	diets	1622:1626	Milk protein concentration was affected by straw type and starch level, and it was 4 and 3% higher when NIS and high-starch diets were fed, respectively.
32921469	3	9	theme	matter	858:863	arg1	basis					870:874	a dry matter (DM) basis	852:874	a dry matter (DM) basis	852:874	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	1	10	theme	different	154:162	arg1	sources					196:202	different dietary neutral detergent fiber sources	154:202	different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations	154:285	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	3	11	theme	hydroxide	965:973	arg1	pellets					994:1000	42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets	880:1000	42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets	880:1000	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	6	12	theme	milk	1486:1489	arg1	yield					1491:1495	a 1.7 kg/d higher milk yield	1468:1495	a 1.7 kg/d higher milk yield	1468:1495	A 1.6 kg/d higher DMI was seen when NIS was fed compared with untreated straw, resulting in a 1.7 kg/d higher milk yield.
32921469	2	13	used	used	659:662	arg2	cows					446:449	Holstein-Friesian cows	428:449	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows	428:585	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	1	14	theme	neutral	172:178	arg1	sources					196:202	different dietary neutral detergent fiber sources	154:202	different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations	154:285	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	2	15	theme	=	481:481	arg1	n					479:479	n = 12	479:484	n = 12	479:484	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	3	16	with	diets	797:801	arg1	level					1115:1119	low or high starch level	1096:1119	low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively)	1096:1176	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	1	17	theme	total-tract	343:353	arg1	digestibility					355:367	total-tract digestibility	343:367	total-tract digestibility	343:367	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	1	18	theme	fiber	190:194	arg1	sources					196:202	different dietary neutral detergent fiber sources	154:202	different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations	154:285	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	3	19	theme	wheat	982:986	arg1	pellets					994:1000	42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets	880:1000	42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets	880:1000	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	2	20	from	cows	446:449	arg1	cows					582:585	early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows	454:585	early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows	454:585	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	5	21	theme	matter	1285:1290	arg1	intake					1292:1297	Dry matter intake	1281:1297	Dry matter intake	1281:1297	Dry matter intake and milk yield were both affected by the type of straw included in the diet.
32921469	4	22	theme	sampling	1271:1278	arg1	period					1253:1258	a 21-d adaptation period	1235:1258	a 21-d adaptation period	1235:1258	Four experimental periods were used, each consisting of a 21-d adaptation period and 7 d of sampling.
32921469	4	22	theme	sampling	1271:1278	arg1	d					1266:1266	7 d	1264:1266	7 d	1264:1266	Four experimental periods were used, each consisting of a 21-d adaptation period and 7 d of sampling.
32921469	9	23	theme	high-producing	1779:1792	arg1	cows					1810:1813	high-producing lactating dairy cows	1779:1813	high-producing lactating dairy cows fed low or high starch concentrations	1779:1851	These results illustrate that feeding NIS to high-producing lactating dairy cows fed low or high starch concentrations has a positive effect on performance.
32921469	0	24	theme	dairy	55:59	arg1	cow					61:63	dairy cow	55:63	dairy cow	55:63	Effects of addition of nutritionally improved straw in dairy cow diets at 2 starch levels.
32921469	3	25	theme	sodium	958:963	arg1	hydroxide					965:973	sodium hydroxide	958:973	sodium hydroxide	958:973	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	2	26	theme	Holstein-Friesian	428:444	arg1	cows					446:449	Holstein-Friesian cows	428:449	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows	428:585	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	9	27	theme	dairy	1804:1808	arg1	cows					1810:1813	high-producing lactating dairy cows	1779:1813	high-producing lactating dairy cows fed low or high starch concentrations	1779:1851	These results illustrate that feeding NIS to high-producing lactating dairy cows fed low or high starch concentrations has a positive effect on performance.
32921469	7	28	theme	starch	1556:1561	arg1	level					1563:1567	starch level	1556:1567	starch level	1556:1567	Milk protein concentration was affected by straw type and starch level, and it was 4 and 3% higher when NIS and high-starch diets were fed, respectively.
32921469	0	29	from	Effects	0:6	arg1	cow					61:63	dairy cow	55:63	dairy cow	55:63	Effects of addition of nutritionally improved straw in dairy cow diets at 2 starch levels.
32921469	1	30	theme	matter	322:327	arg1	intake					329:334	dry matter intake	318:334	dry matter intake (DMI)	318:340	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	1	30	theme	matter	322:327	arg1	DMI					337:339	DMI	337:339	DMI	337:339	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	3	31	theme	DM	866:867	arg1	basis					870:874	a dry matter (DM) basis	852:874	a dry matter (DM) basis	852:874	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	0	32	from	straw	46:50	arg1	cow					61:63	dairy cow	55:63	dairy cow	55:63	Effects of addition of nutritionally improved straw in dairy cow diets at 2 starch levels.
32921469	2	33	theme	experiment	534:543	arg1	start					521:525	the start	517:525	the start of the experiment	517:543	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	3	34	theme	different	1068:1076	arg1	concentrates					1078:1089	1 of 2 different concentrates	1061:1089	1 of 2 different concentrates	1061:1089	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	3	35	theme	%	884:884	arg1	NaOH					976:979	NaOH	976:979	NaOH	976:979	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	3	35	theme	%	884:884	arg1	silage					892:897	42.4% grass silage	880:897	42.4% grass silage as the main forage	880:916	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	3	36	theme	starch	1108:1113	arg1	level					1115:1119	low or high starch level	1096:1119	low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively)	1096:1176	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	9	37	theme	starch	1831:1836	arg1	concentrations					1838:1851	low or high starch concentrations	1819:1851	low or high starch concentrations	1819:1851	These results illustrate that feeding NIS to high-producing lactating dairy cows fed low or high starch concentrations has a positive effect on performance.
32921469	1	38	theme	low	253:255	arg1	concentrations					272:285	low or high starch concentrations	253:285	low or high starch concentrations	253:285	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	2	39	dep	=	590:590	arg1	kg					604:605	4; 878 ± 67 kg	592:605	4; 878 ± 67 kg	592:605	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	2	40	theme	cannulated	554:563	arg1	cows					582:585	early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows	454:585	early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows	454:585	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	0	41	theme	addition	11:18	arg1	Effects					0:6	Effects	0:6	Effects of addition of nutritionally improved straw in dairy cow	0:63	Effects of addition of nutritionally improved straw in dairy cow diets at 2 starch levels.
32921469	1	42	theme	high	260:263	arg1	concentrations					272:285	low or high starch concentrations	253:285	low or high starch concentrations	253:285	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	7	43	theme	protein	1503:1509	arg1	concentration					1511:1523	Milk protein concentration	1498:1523	Milk protein concentration	1498:1523	Milk protein concentration was affected by straw type and starch level, and it was 4 and 3% higher when NIS and high-starch diets were fed, respectively.
32921469	3	44	theme	starch	1126:1131	arg1	level					1133:1137	TMR starch level	1122:1137	TMR starch level of 16.0 vs. 24.0% of DM, respectively	1122:1175	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	9	45	theme	positive	1859:1866	arg1	effect					1868:1873	a positive effect	1857:1873	a positive effect on performance	1857:1888	These results illustrate that feeding NIS to high-producing lactating dairy cows fed low or high starch concentrations has a positive effect on performance.
32921469	3	46	theme	silage	892:897	arg1	pellets					994:1000	42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets	880:1000	42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets	880:1000	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	1	47	with	cattle	241:246	arg1	concentrations					272:285	low or high starch concentrations	253:285	low or high starch concentrations	253:285	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	2	48	dep	mid-lactation	464:476	arg1	n					479:479	n = 12	479:484	n = 12	479:484	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	1	49	theme	milk	290:293	arg1	yield					295:299	milk yield	290:299	milk yield	290:299	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	3	50	theme	forage-to-concentrate	775:795	arg1	treatments					753:762	The treatments	749:762	The treatments	749:762	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	3	50	theme	forage-to-concentrate	775:795	arg1	diets					797:801	50:50 forage-to-concentrate diets	769:801	50:50 forage-to-concentrate diets	769:801	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	6	51	theme	higher	1387:1392	arg1	DMI					1394:1396	A 1.6 kg/d higher DMI	1376:1396	A 1.6 kg/d higher DMI	1376:1396	A 1.6 kg/d higher DMI was seen when NIS was fed compared with untreated straw, resulting in a 1.7 kg/d higher milk yield.
32921469	2	52	from	start	629:633	arg1	weight					615:620	body weight	610:620	body weight at the start of the experiment	610:651	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	2	52	from	start	629:633	arg1	n					588:588	n	588:588	n = 4; 878 ± 67 kg	588:605	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	1	53	theme	sources	196:202	arg1	effects					143:149	the effects	139:149	the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health	139:425	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	2	54	theme	square	688:693	arg1	design					695:700	4 × 4 Latin square design	676:700	multiple 4 × 4 Latin square design experiment	667:711	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	3	55	theme	improved	1026:1033	arg1	NIS					1042:1044	NIS	1042:1044	NIS	1042:1044	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	3	55	theme	improved	1026:1033	arg1	straw					1035:1039	nutritionally improved straw	1012:1039	nutritionally improved straw (NIS)	1012:1045	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	3	56	theme	wheat	942:946	arg1	straw					948:952	7.6% chopped untreated wheat straw	919:952	7.6% chopped untreated wheat straw	919:952	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	3	57	theme	total	812:816	arg1	TMR					832:834	TMR	832:834	TMR	832:834	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	3	57	theme	total	812:816	arg1	ration					824:829	a total mixed ration	810:829	a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS),	810:1046	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	2	58	theme	×	678:678	arg1	design					695:700	4 × 4 Latin square design	676:700	multiple 4 × 4 Latin square design experiment	667:711	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	1	59	theme	high-producing	220:233	arg1	cattle					241:246	high-producing dairy cattle	220:246	high-producing dairy cattle with low or high starch concentrations	220:285	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	3	60	theme	chopped	924:930	arg1	straw					948:952	7.6% chopped untreated wheat straw	919:952	7.6% chopped untreated wheat straw	919:952	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	2	61	theme	weight	507:512	arg1	kg					496:497	666 ± 67 kg	487:497	666 ± 67 kg of body weight at the start of the experiment	487:543	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	3	62	dep	level	1115:1119	arg1	level					1133:1137	TMR starch level	1122:1137	TMR starch level of 16.0 vs. 24.0% of DM, respectively	1122:1175	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	2	63	theme	multiple	667:674	arg1	experiment					702:711	multiple 4 × 4 Latin square design experiment	667:711	multiple 4 × 4 Latin square design experiment	667:711	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	6	64	theme	untreated	1438:1446	arg1	straw					1448:1452	untreated straw	1438:1452	untreated straw	1438:1452	A 1.6 kg/d higher DMI was seen when NIS was fed compared with untreated straw, resulting in a 1.7 kg/d higher milk yield.
32921469	1	65	theme	cattle	241:246	arg1	diets					211:215	diets	211:215	diets of high-producing dairy cattle with low or high starch concentrations	211:285	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	2	66	dep	n	479:479	arg1	kg					496:497	666 ± 67 kg	487:497	666 ± 67 kg of body weight at the start of the experiment	487:543	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	0	67	theme	straw	46:50	arg1	Effects					0:6	Effects	0:6	Effects of addition of nutritionally improved straw in dairy cow	0:63	Effects of addition of nutritionally improved straw in dairy cow diets at 2 starch levels.
32921469	3	68	theme	dry	854:856	arg1	basis					870:874	a dry matter (DM) basis	852:874	a dry matter (DM) basis	852:874	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	2	69	theme	body	610:613	arg1	weight					615:620	body weight	610:620	body weight at the start of the experiment	610:651	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	2	70	from	n	588:588	arg1	start					629:633	the start	625:633	the start of the experiment	625:651	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	4	71	used	used	1210:1213	arg2	periods					1197:1203	Four experimental periods	1179:1203	Four experimental periods	1179:1203	Four experimental periods were used, each consisting of a 21-d adaptation period and 7 d of sampling.
32921469	4	72	theme	21-d	1237:1240	arg1	period					1253:1258	a 21-d adaptation period	1235:1258	a 21-d adaptation period	1235:1258	Four experimental periods were used, each consisting of a 21-d adaptation period and 7 d of sampling.
32921469	9	73	contain	has	1853:1855	arg2	effect					1868:1873	a positive effect	1857:1873	a positive effect on performance	1857:1888	These results illustrate that feeding NIS to high-producing lactating dairy cows fed low or high starch concentrations has a positive effect on performance.
32921469	9	73	contain	has	1853:1855	arg1	NIS					1772:1774	feeding NIS	1764:1774	feeding NIS to high-producing lactating dairy cows fed low or high starch concentrations	1764:1851	These results illustrate that feeding NIS to high-producing lactating dairy cows fed low or high starch concentrations has a positive effect on performance.
32921469	2	74	dep	used	659:662	arg1	n					588:588	n	588:588	n = 4; 878 ± 67 kg	588:605	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	5	75	theme	milk	1303:1306	arg1	yield					1308:1312	milk yield	1303:1312	milk yield	1303:1312	Dry matter intake and milk yield were both affected by the type of straw included in the diet.
32921469	1	76	theme	dietary	164:170	arg1	sources					196:202	different dietary neutral detergent fiber sources	154:202	different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations	154:285	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	3	77	theme	DM	1160:1161	arg1	DM					1160:1161	DM	1160:1161	DM	1160:1161	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	3	77	theme	DM	1160:1161	arg1	%					1155:1155	16.0 vs. 24.0%	1142:1155	16.0 vs. 24.0%	1142:1155	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	7	78	theme	NIS	1602:1604	arg1	diets					1622:1626	NIS and high-starch diets	1602:1626	diets	1622:1626	Milk protein concentration was affected by straw type and starch level, and it was 4 and 3% higher when NIS and high-starch diets were fed, respectively.
32921469	6	79	theme	higher	1479:1484	arg1	yield					1491:1495	a 1.7 kg/d higher milk yield	1468:1495	a 1.7 kg/d higher milk yield	1468:1495	A 1.6 kg/d higher DMI was seen when NIS was fed compared with untreated straw, resulting in a 1.7 kg/d higher milk yield.
32921469	1	80	theme	detergent	180:188	arg1	sources					196:202	different dietary neutral detergent fiber sources	154:202	different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations	154:285	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	3	81	theme	%	1155:1155	arg1	level					1133:1137	TMR starch level	1122:1137	TMR starch level of 16.0 vs. 24.0% of DM, respectively	1122:1175	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	5	82	theme	Dry	1281:1283	arg1	intake					1292:1297	Dry matter intake	1281:1297	Dry matter intake	1281:1297	Dry matter intake and milk yield were both affected by the type of straw included in the diet.
32921469	3	83	dep	main	906:909	arg1	forage					911:916	forage	911:916	forage	911:916	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	9	84	from	effect	1868:1873	arg1	performance					1878:1888	performance	1878:1888	performance	1878:1888	These results illustrate that feeding NIS to high-producing lactating dairy cows fed low or high starch concentrations has a positive effect on performance.
32921469	3	85	theme	straw	988:992	arg1	pellets					994:1000	42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets	880:1000	42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets	880:1000	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	2	86	theme	67	493:494	arg1	±					491:491	±	491:491	±	491:491	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	2	87	theme	=	590:590	arg1	n					588:588	n	588:588	n = 4; 878 ± 67 kg	588:605	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	9	88	theme	feeding	1764:1770	arg1	NIS					1772:1774	feeding NIS	1764:1774	feeding NIS to high-producing lactating dairy cows fed low or high starch concentrations	1764:1851	These results illustrate that feeding NIS to high-producing lactating dairy cows fed low or high starch concentrations has a positive effect on performance.
32921469	1	89	theme	nitrogen	370:377	arg1	partitioning					383:394	nitrogen (N) partitioning	370:394	nitrogen (N) partitioning	370:394	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	2	90	theme	666	487:489	arg1	±					491:491	±	491:491	±	491:491	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	1	91	theme	N	380:380	arg1	partitioning					383:394	nitrogen (N) partitioning	370:394	nitrogen (N) partitioning	370:394	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	1	92	theme	experiment	113:122	arg1	objective					95:103	The objective	91:103	The objective of this experiment	91:122	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	2	93	theme	±	599:599	arg1	kg					604:605	4; 878 ± 67 kg	592:605	4; 878 ± 67 kg	592:605	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	9	94	theme	lactating	1794:1802	arg1	cows					1810:1813	high-producing lactating dairy cows	1779:1813	high-producing lactating dairy cows fed low or high starch concentrations	1779:1851	These results illustrate that feeding NIS to high-producing lactating dairy cows fed low or high starch concentrations has a positive effect on performance.
32921469	1	95	from	effects	143:149	arg1	digestibility					355:367	total-tract digestibility	343:367	total-tract digestibility	343:367	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	1	95	from	effects	143:149	arg1	partitioning					383:394	nitrogen (N) partitioning	370:394	nitrogen (N) partitioning	370:394	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	1	95	from	effects	143:149	arg1	yield					295:299	milk yield	290:299	milk yield	290:299	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	1	95	from	effects	143:149	arg1	intake					329:334	dry matter intake	318:334	dry matter intake (DMI)	318:340	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	1	95	from	effects	143:149	arg1	DMI					337:339	DMI	337:339	DMI	337:339	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	1	95	from	effects	143:149	arg1	composition					305:315	composition	305:315	composition	305:315	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	1	95	from	effects	143:149	arg1	function					407:414	rumen function	401:414	rumen function	401:414	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	2	96	theme	dry	550:552	arg1	cows					582:585	early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows	454:585	early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows	454:585	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	0	97	theme	starch	76:81	arg1	levels					83:88	2 starch levels	74:88	2 starch levels	74:88	Effects of addition of nutritionally improved straw in dairy cow diets at 2 starch levels.
32921469	1	98	theme	dry	318:320	arg1	intake					329:334	dry matter intake	318:334	dry matter intake (DMI)	318:340	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	1	98	theme	dry	318:320	arg1	DMI					337:339	DMI	337:339	DMI	337:339	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	7	99	theme	straw	1541:1545	arg1	type					1547:1550	straw type	1541:1550	straw type	1541:1550	Milk protein concentration was affected by straw type and starch level, and it was 4 and 3% higher when NIS and high-starch diets were fed, respectively.
32921469	4	100	theme	adaptation	1242:1251	arg1	period					1253:1258	a 21-d adaptation period	1235:1258	a 21-d adaptation period	1235:1258	Four experimental periods were used, each consisting of a 21-d adaptation period and 7 d of sampling.
32921469	3	101	theme	low	1096:1098	arg1	level					1115:1119	low or high starch level	1096:1119	low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively)	1096:1176	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	9	102	theme	low	1819:1821	arg1	concentrations					1838:1851	low or high starch concentrations	1819:1851	low or high starch concentrations	1819:1851	These results illustrate that feeding NIS to high-producing lactating dairy cows fed low or high starch concentrations has a positive effect on performance.
32921469	2	103	theme	early-	454:459	arg1	cows					582:585	early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows	454:585	early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows	454:585	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	3	104	theme	concentrates	1078:1089	arg1	TMR					832:834	TMR	832:834	TMR	832:834	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	3	104	theme	concentrates	1078:1089	arg1	%					1056:1056	50.0%	1052:1056	50.0%	1052:1056	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	3	104	theme	concentrates	1078:1089	arg1	ration					824:829	a total mixed ration	810:829	a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS),	810:1046	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	3	104	theme	concentrates	1078:1089	arg1	concentrates					1078:1089	1 of 2 different concentrates	1061:1089	1 of 2 different concentrates	1061:1089	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	9	105	theme	high	1826:1829	arg1	concentrations					1838:1851	low or high starch concentrations	1819:1851	low or high starch concentrations	1819:1851	These results illustrate that feeding NIS to high-producing lactating dairy cows fed low or high starch concentrations has a positive effect on performance.
32921469	8	106	with	Diets	1652:1656	arg1	NIS					1663:1665	NIS	1663:1665	NIS	1663:1665	Diets with NIS were more positively effective when fed with low levels of starch.
32921469	2	107	theme	Holstein-Frisian	565:580	arg1	cows					582:585	early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows	454:585	early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows	454:585	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	5	108	theme	straw	1348:1352	arg1	type					1340:1343	the type	1336:1343	the type of straw included in the diet	1336:1373	Dry matter intake and milk yield were both affected by the type of straw included in the diet.
32921469	7	109	theme	Milk	1498:1501	arg1	concentration					1511:1523	Milk protein concentration	1498:1523	Milk protein concentration	1498:1523	Milk protein concentration was affected by straw type and starch level, and it was 4 and 3% higher when NIS and high-starch diets were fed, respectively.
32921469	3	110	theme	50:50	769:773	arg1	treatments					753:762	The treatments	749:762	The treatments	749:762	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	3	110	theme	50:50	769:773	arg1	diets					797:801	50:50 forage-to-concentrate diets	769:801	50:50 forage-to-concentrate diets	769:801	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	2	111	theme	different	732:740	arg1	diets					742:746	4 different diets	730:746	4 different diets	730:746	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	3	112	theme	high	1103:1106	arg1	level					1115:1119	low or high starch level	1096:1119	low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively)	1096:1176	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	0	113	theme	improved	37:44	arg1	straw					46:50	nutritionally improved straw	23:50	nutritionally improved straw in dairy cow	23:63	Effects of addition of nutritionally improved straw in dairy cow diets at 2 starch levels.
32921469	1	114	theme	starch	265:270	arg1	concentrations					272:285	low or high starch concentrations	253:285	low or high starch concentrations	253:285	The objective of this experiment was to explore the effects of different dietary neutral detergent fiber sources within diets of high-producing dairy cattle with low or high starch concentrations on milk yield and composition, dry matter intake (DMI), total-tract digestibility, nitrogen (N) partitioning, and rumen function and health.
32921469	3	115	theme	grass	886:890	arg1	NaOH					976:979	NaOH	976:979	NaOH	976:979	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	3	115	theme	grass	886:890	arg1	silage					892:897	42.4% grass silage	880:897	42.4% grass silage as the main forage	880:916	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	2	116	from	start	521:525	arg1	kg					496:497	666 ± 67 kg	487:497	666 ± 67 kg of body weight at the start of the experiment	487:543	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	8	117	theme	low	1712:1714	arg1	levels					1716:1721	low levels	1712:1721	low levels of starch	1712:1731	Diets with NIS were more positively effective when fed with low levels of starch.
32921469	3	118	theme	TMR	1122:1124	arg1	level					1133:1137	TMR starch level	1122:1137	TMR starch level of 16.0 vs. 24.0% of DM, respectively	1122:1175	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	0	119	from	cow	61:63	arg1	Effects					0:6	Effects	0:6	Effects of addition of nutritionally improved straw in dairy cow	0:63	Effects of addition of nutritionally improved straw in dairy cow diets at 2 starch levels.
32921469	2	120	theme	design	695:700	arg1	experiment					702:711	multiple 4 × 4 Latin square design experiment	667:711	multiple 4 × 4 Latin square design experiment	667:711	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	3	121	theme	untreated	932:940	arg1	straw					948:952	7.6% chopped untreated wheat straw	919:952	7.6% chopped untreated wheat straw	919:952	The treatments were 50:50 forage-to-concentrate diets within a total mixed ration (TMR) consisting, on a dry matter (DM) basis, of 42.4% grass silage as the main forage, 7.6% chopped untreated wheat straw, or sodium hydroxide (NaOH) wheat straw pellets, known as nutritionally improved straw (NIS), and 50.0% of 1 of 2 different concentrates with low or high starch level (TMR starch level of 16.0 vs. 24.0% of DM, respectively).
32921469	2	122	theme	experiment	642:651	arg1	start					629:633	the start	625:633	the start of the experiment	625:651	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	2	123	theme	Latin	682:686	arg1	design					695:700	4 × 4 Latin square design	676:700	multiple 4 × 4 Latin square design experiment	667:711	Holstein-Friesian cows in early- to mid-lactation (n = 12; 666 ± 67 kg of body weight at the start of the experiment) and dry cannulated Holstein-Frisian cows (n = 4; 878 ± 67 kg of body weight at the start of the experiment) were used in multiple 4 × 4 Latin square design experiment and were offered 4 different diets.
32921469	4	124	theme	experimental	1184:1195	arg1	periods					1197:1203	Four experimental periods	1179:1203	Four experimental periods	1179:1203	Four experimental periods were used, each consisting of a 21-d adaptation period and 7 d of sampling.
32737807	15	0	theme	RG	1745:1746	arg1	pathway					1767:1773	RG I pectin-degrading pathway	1745:1773	RG I pectin-degrading pathway	1745:1773	strain PAMC 28499 have similar patterns to RG I pectin-degrading pathway.
32737807	4	1	with	region	236:241	arg1	surviving					316:324	living creatures surviving	299:324	living creatures surviving	299:324	Uganda is a unique region where hot areas and glaciers coexist, with a variety of living creatures surviving, but the survey on them is very poor.
32737807	4	1	with	region	236:241	arg1	variety					288:294	a variety	286:294	a variety of living creatures surviving	286:324	Uganda is a unique region where hot areas and glaciers coexist, with a variety of living creatures surviving, but the survey on them is very poor.
32737807	12	2	with	CAZymes	1342:1348	arg1	esterases					1369:1377	extracellular esterases	1355:1377	extracellular esterases	1355:1377	Its genome encodes a simple set of metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases.
32737807	12	2	with	CAZymes	1342:1348	arg1	lyases					1391:1396	pectate lyases	1383:1396	pectate lyases	1383:1396	Its genome encodes a simple set of metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases.
32737807	9	3	theme	NCBI	921:924	arg1	database					940:947	the program NCBI non-redundant database	909:947	the program NCBI non-redundant database	909:947	The predicted gene sequences were functionally annotated and gene prediction was carried out using the program NCBI non-redundant database.
32737807	13	4	theme	annotation	1406:1415	arg1	analyses					1417:1424	CAZyme annotation analyses	1399:1424	CAZyme annotation analyses	1399:1424	CAZyme annotation analyses revealed 165 genes related to carbohydrate active, and especially we have found GH1, GH2, GH3, GH38, GH35, GH51, GH51, GH53, GH106, GH146, CE12, PL1 and PL11 such as known pectin degradation genes from Sphingobium yanoikuiae.
32737807	18	5	theme	comparative	1897:1907	arg1	analysis					1916:1923	comparative genome analysis	1897:1923	comparative genome analysis	1897:1923	Also, this strain has comparative genome analysis.
32737807	5	6	theme	environmental	451:463	arg1	studies					465:471	environmental studies	451:471	environmental studies	451:471	The genetic character and complete genome information of Sphingobium strains help with environmental studies and the development of better to enzyme industry.
32737807	7	7	theme	region	686:691	arg1	variety					671:677	variety	671:677	variety of the region	671:691	PAMC 28499 and comparative analysis of Spingobium species strains isolated from variety of the region.
32737807	7	7	theme	region	686:691	arg1	region					686:691	the region	682:691	the region	682:691	PAMC 28499 and comparative analysis of Spingobium species strains isolated from variety of the region.
32737807	12	8	theme	pectate	1383:1389	arg1	lyases					1391:1396	pectate lyases	1383:1396	pectate lyases	1383:1396	Its genome encodes a simple set of metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases.
32737807	15	9	theme	similar	1725:1731	arg1	patterns					1733:1740	similar patterns	1725:1740	similar patterns	1725:1740	strain PAMC 28499 have similar patterns to RG I pectin-degrading pathway.
32737807	2	10	theme	BACKGROUND	139:148	arg1	sp					161:162	BACKGROUND Spingobium sp	139:162	BACKGROUND Spingobium sp.	139:163	BACKGROUND Spingobium sp.
32737807	15	11	theme	I	1748:1748	arg1	pathway					1767:1773	RG I pectin-degrading pathway	1745:1773	RG I pectin-degrading pathway	1745:1773	strain PAMC 28499 have similar patterns to RG I pectin-degrading pathway.
32737807	18	12	theme	genome	1909:1914	arg1	analysis					1916:1923	comparative genome analysis	1897:1923	comparative genome analysis	1897:1923	Also, this strain has comparative genome analysis.
32737807	7	13	theme	species	641:647	arg1	28499					596:600	PAMC 28499	591:600	PAMC 28499	591:600	PAMC 28499 and comparative analysis of Spingobium species strains isolated from variety of the region.
32737807	7	13	theme	species	641:647	arg1	analysis					618:625	comparative analysis	606:625	comparative analysis	606:625	PAMC 28499 and comparative analysis of Spingobium species strains isolated from variety of the region.
32737807	5	14	theme	Sphingobium	421:431	arg1	strains					433:439	Sphingobium strains	421:439	Sphingobium strains	421:439	The genetic character and complete genome information of Sphingobium strains help with environmental studies and the development of better to enzyme industry.
32737807	4	15	from	survey	335:340	arg1	them					345:348	them	345:348	them	345:348	Uganda is a unique region where hot areas and glaciers coexist, with a variety of living creatures surviving, but the survey on them is very poor.
32737807	6	16	theme	complete	548:555	arg1	sequence					564:571	complete genome sequence	548:571	complete genome sequence of Spingobium sp	548:588	OBJECTIVE In this study, complete genome sequence of Spingobium sp.
32737807	6	16	theme	complete	548:555	arg1	OBJECTIVE					523:531	OBJECTIVE	523:531	OBJECTIVE In this study	523:545	OBJECTIVE In this study, complete genome sequence of Spingobium sp.
32737807	8	17	theme	METHODS	694:700	arg1	sequencing					709:718	METHODS Genome sequencing	694:718	METHODS Genome sequencing	694:718	METHODS Genome sequencing was performed using PacBio sequel single-molecule real-time (SMRT) sequencing technology.
32737807	10	18	theme	protein	1025:1031	arg1	prediction					1033:1042	protein prediction	1025:1042	protein prediction about carbohydrate active enzymes (CAZymes)	1025:1086	And using dbCAN2 and KEGG data base were degradation pathway predicted and protein prediction about carbohydrate active enzymes (CAZymes).
32737807	11	19	theme	genome	1101:1106	arg1	sequence					1108:1115	The genome sequence	1097:1115	The genome sequence	1097:1115	RESULTS The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons.
32737807	5	20	theme	enzyme	506:511	arg1	industry					513:520	enzyme industry	506:520	enzyme industry	506:520	The genetic character and complete genome information of Sphingobium strains help with environmental studies and the development of better to enzyme industry.
32737807	13	21	dep	PL1	1571:1573	arg1	GH35					1527:1530	GH35	1527:1530	GH35	1527:1530	CAZyme annotation analyses revealed 165 genes related to carbohydrate active, and especially we have found GH1, GH2, GH3, GH38, GH35, GH51, GH51, GH53, GH106, GH146, CE12, PL1 and PL11 such as known pectin degradation genes from Sphingobium yanoikuiae.
32737807	13	21	dep	PL1	1571:1573	arg1	GH53					1545:1548	GH53	1545:1548	GH53	1545:1548	CAZyme annotation analyses revealed 165 genes related to carbohydrate active, and especially we have found GH1, GH2, GH3, GH38, GH35, GH51, GH51, GH53, GH106, GH146, CE12, PL1 and PL11 such as known pectin degradation genes from Sphingobium yanoikuiae.
32737807	13	21	dep	PL1	1571:1573	arg1	GH106					1551:1555	GH106	1551:1555	GH106	1551:1555	CAZyme annotation analyses revealed 165 genes related to carbohydrate active, and especially we have found GH1, GH2, GH3, GH38, GH35, GH51, GH51, GH53, GH106, GH146, CE12, PL1 and PL11 such as known pectin degradation genes from Sphingobium yanoikuiae.
32737807	13	21	dep	PL1	1571:1573	arg1	GH51					1533:1536	GH51	1533:1536	GH51	1533:1536	CAZyme annotation analyses revealed 165 genes related to carbohydrate active, and especially we have found GH1, GH2, GH3, GH38, GH35, GH51, GH51, GH53, GH106, GH146, CE12, PL1 and PL11 such as known pectin degradation genes from Sphingobium yanoikuiae.
32737807	13	21	dep	PL1	1571:1573	arg1	GH51					1539:1542	GH51	1539:1542	GH51	1539:1542	CAZyme annotation analyses revealed 165 genes related to carbohydrate active, and especially we have found GH1, GH2, GH3, GH38, GH35, GH51, GH51, GH53, GH106, GH146, CE12, PL1 and PL11 such as known pectin degradation genes from Sphingobium yanoikuiae.
32737807	13	21	dep	PL1	1571:1573	arg1	GH146					1558:1562	GH146	1558:1562	GH146	1558:1562	CAZyme annotation analyses revealed 165 genes related to carbohydrate active, and especially we have found GH1, GH2, GH3, GH38, GH35, GH51, GH51, GH53, GH106, GH146, CE12, PL1 and PL11 such as known pectin degradation genes from Sphingobium yanoikuiae.
32737807	13	21	dep	PL1	1571:1573	arg1	CE12					1565:1568	CE12	1565:1568	CE12	1565:1568	CAZyme annotation analyses revealed 165 genes related to carbohydrate active, and especially we have found GH1, GH2, GH3, GH38, GH35, GH51, GH51, GH53, GH106, GH146, CE12, PL1 and PL11 such as known pectin degradation genes from Sphingobium yanoikuiae.
32737807	12	22	theme	unusual	1318:1324	arg1	distribution					1326:1337	an unusual distribution	1315:1337	an unusual distribution	1315:1337	Its genome encodes a simple set of metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases.
32737807	10	23	theme	active	1063:1068	arg1	CAZymes					1079:1085	CAZymes	1079:1085	CAZymes	1079:1085	And using dbCAN2 and KEGG data base were degradation pathway predicted and protein prediction about carbohydrate active enzymes (CAZymes).
32737807	10	23	theme	active	1063:1068	arg1	enzymes					1070:1076	carbohydrate active enzymes	1050:1076	carbohydrate active enzymes (CAZymes)	1050:1086	And using dbCAN2 and KEGG data base were degradation pathway predicted and protein prediction about carbohydrate active enzymes (CAZymes).
32737807	1	24	theme	comparative	108:118	arg1	approach					129:136	comparative genomics approach	108:136	comparative genomics approach	108:136	strain PAMC 28499 reveals a potential for degrading pectin with comparative genomics approach.
32737807	8	25	theme	PacBio	740:745	arg1	technology					798:807	PacBio sequel single-molecule real-time (SMRT) sequencing technology	740:807	PacBio sequel single-molecule real-time (SMRT) sequencing technology	740:807	METHODS Genome sequencing was performed using PacBio sequel single-molecule real-time (SMRT) sequencing technology.
32737807	11	26	dep	RESULTS	1089:1095	arg1	has					1117:1119	has	1117:1119	has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons	1117:1201	RESULTS The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons.
32737807	20	27	theme	new	2088:2090	arg1	genes					2092:2096	new genes	2088:2096	new genes that degradation the pectin	2088:2124	It can also provide bioengineered data by finding new genes that degradation the pectin.
32737807	12	28	theme	predicted	1285:1293	arg1	protein					1307:1313	its predicted degradation protein	1281:1313	its predicted degradation protein	1281:1313	Its genome encodes a simple set of metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases.
32737807	8	29	theme	single-molecule	754:768	arg1	technology					798:807	PacBio sequel single-molecule real-time (SMRT) sequencing technology	740:807	PacBio sequel single-molecule real-time (SMRT) sequencing technology	740:807	METHODS Genome sequencing was performed using PacBio sequel single-molecule real-time (SMRT) sequencing technology.
32737807	7	30	theme	comparative	606:616	arg1	analysis					618:625	comparative analysis	606:625	comparative analysis	606:625	PAMC 28499 and comparative analysis of Spingobium species strains isolated from variety of the region.
32737807	5	31	theme	better	496:501	arg1	studies					465:471	environmental studies	451:471	environmental studies	451:471	The genetic character and complete genome information of Sphingobium strains help with environmental studies and the development of better to enzyme industry.
32737807	5	31	theme	better	496:501	arg1	development					481:491	the development	477:491	the development of better to enzyme industry	477:520	The genetic character and complete genome information of Sphingobium strains help with environmental studies and the development of better to enzyme industry.
32737807	13	32	theme	pectin	1598:1603	arg1	genes					1617:1621	known pectin degradation genes	1592:1621	known pectin degradation genes from Sphingobium yanoikuiae	1592:1649	CAZyme annotation analyses revealed 165 genes related to carbohydrate active, and especially we have found GH1, GH2, GH3, GH38, GH35, GH51, GH51, GH53, GH106, GH146, CE12, PL1 and PL11 such as known pectin degradation genes from Sphingobium yanoikuiae.
32737807	6	33	theme	Spingobium	576:585	arg1	sp					587:588	Spingobium sp	576:588	Spingobium sp	576:588	OBJECTIVE In this study, complete genome sequence of Spingobium sp.
32737807	14	34	dep	confirmed	1666:1674	arg1	sp					1698:1699	this Sphingobium sp	1681:1699	this Sphingobium sp	1681:1699	These results confirmed that this Sphingobium sp.
32737807	7	35	dep	strains	649:655	arg1	isolated					657:664	isolated	657:664	strains isolated from variety of the region	649:691	PAMC 28499 and comparative analysis of Spingobium species strains isolated from variety of the region.
32737807	9	36	theme	predicted	814:822	arg1	sequences					829:837	The predicted gene sequences	810:837	The predicted gene sequences	810:837	The predicted gene sequences were functionally annotated and gene prediction was carried out using the program NCBI non-redundant database.
32737807	18	37	contain	has	1893:1895	arg1	strain					1886:1891	this strain	1881:1891	this strain	1881:1891	Also, this strain has comparative genome analysis.
32737807	18	37	contain	has	1893:1895	arg2	analysis					1916:1923	comparative genome analysis	1897:1923	comparative genome analysis	1897:1923	Also, this strain has comparative genome analysis.
32737807	0	38	theme	sp	40:41	arg1	sequence					16:23	Complete genome sequence	0:23	Complete genome sequence of Sphingobium sp	0:41	Complete genome sequence of Sphingobium sp.
32737807	11	39	theme	protein	1151:1157	arg1	content					1130:1136	64.5% GC content	1121:1136	64.5% GC content	1121:1136	RESULTS The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons.
32737807	11	39	theme	protein	1151:1157	arg1	genes					1166:1170	4432 coding protein coding genes	1139:1170	4432 coding protein coding genes	1139:1170	RESULTS The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons.
32737807	12	40	theme	metabolic	1239:1247	arg1	pathways					1249:1256	metabolic pathways	1239:1256	metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases	1239:1396	Its genome encodes a simple set of metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases.
32737807	15	41	theme	strain	1702:1707	arg1	28499					1714:1718	strain PAMC 28499	1702:1718	strain PAMC 28499	1702:1718	strain PAMC 28499 have similar patterns to RG I pectin-degrading pathway.
32737807	0	42	theme	Complete	0:7	arg1	sequence					16:23	Complete genome sequence	0:23	Complete genome sequence of Sphingobium sp	0:41	Complete genome sequence of Sphingobium sp.
32737807	4	43	theme	hot	249:251	arg1	areas					253:257	hot areas	249:257	hot areas	249:257	Uganda is a unique region where hot areas and glaciers coexist, with a variety of living creatures surviving, but the survey on them is very poor.
32737807	12	44	theme	relevant	1258:1265	arg1	pathways					1249:1256	metabolic pathways	1239:1256	metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases	1239:1396	Its genome encodes a simple set of metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases.
32737807	11	45	theme	rRNA	1190:1193	arg1	content					1130:1136	64.5% GC content	1121:1136	64.5% GC content	1121:1136	RESULTS The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons.
32737807	11	45	theme	rRNA	1190:1193	arg1	operons					1195:1201	12 rRNA operons	1187:1201	12 rRNA operons	1187:1201	RESULTS The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons.
32737807	10	46	theme	KEGG	971:974	arg1	base					981:984	KEGG data base	971:984	KEGG data base	971:984	And using dbCAN2 and KEGG data base were degradation pathway predicted and protein prediction about carbohydrate active enzymes (CAZymes).
32737807	13	47	theme	related	1445:1451	arg1	genes					1439:1443	165 genes	1435:1443	165 genes related to carbohydrate active	1435:1474	CAZyme annotation analyses revealed 165 genes related to carbohydrate active, and especially we have found GH1, GH2, GH3, GH38, GH35, GH51, GH51, GH53, GH106, GH146, CE12, PL1 and PL11 such as known pectin degradation genes from Sphingobium yanoikuiae.
32737807	0	48	theme	Sphingobium	28:38	arg1	sp					40:41	Sphingobium sp	28:41	Sphingobium sp	28:41	Complete genome sequence of Sphingobium sp.
32737807	11	49	theme	%	1125:1125	arg1	genes					1166:1170	4432 coding protein coding genes	1139:1170	4432 coding protein coding genes	1139:1170	RESULTS The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons.
32737807	11	49	theme	%	1125:1125	arg1	content					1130:1136	64.5% GC content	1121:1136	64.5% GC content	1121:1136	RESULTS The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons.
32737807	11	49	theme	%	1125:1125	arg1	operons					1195:1201	12 rRNA operons	1187:1201	12 rRNA operons	1187:1201	RESULTS The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons.
32737807	11	49	theme	%	1125:1125	arg1	tRNAs					1176:1180	61 tRNAs	1173:1180	61 tRNAs	1173:1180	RESULTS The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons.
32737807	4	50	theme	surviving	316:324	arg1	surviving					316:324	living creatures surviving	299:324	living creatures surviving	299:324	Uganda is a unique region where hot areas and glaciers coexist, with a variety of living creatures surviving, but the survey on them is very poor.
32737807	4	50	theme	surviving	316:324	arg1	variety					288:294	a variety	286:294	a variety of living creatures surviving	286:324	Uganda is a unique region where hot areas and glaciers coexist, with a variety of living creatures surviving, but the survey on them is very poor.
32737807	10	51	theme	using	954:958	arg1	dbCAN2					960:965	using dbCAN2	954:965	using dbCAN2	954:965	And using dbCAN2 and KEGG data base were degradation pathway predicted and protein prediction about carbohydrate active enzymes (CAZymes).
32737807	13	52	theme	165	1435:1437	arg1	genes					1439:1443	165 genes	1435:1443	165 genes related to carbohydrate active	1435:1474	CAZyme annotation analyses revealed 165 genes related to carbohydrate active, and especially we have found GH1, GH2, GH3, GH38, GH35, GH51, GH51, GH53, GH106, GH146, CE12, PL1 and PL11 such as known pectin degradation genes from Sphingobium yanoikuiae.
32737807	5	53	theme	genome	399:404	arg1	information					406:416	complete genome information	390:416	complete genome information	390:416	The genetic character and complete genome information of Sphingobium strains help with environmental studies and the development of better to enzyme industry.
32737807	15	54	theme	pectin-degrading	1750:1765	arg1	pathway					1767:1773	RG I pectin-degrading pathway	1745:1773	RG I pectin-degrading pathway	1745:1773	strain PAMC 28499 have similar patterns to RG I pectin-degrading pathway.
32737807	10	55	theme	data	976:979	arg1	base					981:984	KEGG data base	971:984	KEGG data base	971:984	And using dbCAN2 and KEGG data base were degradation pathway predicted and protein prediction about carbohydrate active enzymes (CAZymes).
32737807	4	56	theme	living	299:304	arg1	surviving					316:324	living creatures surviving	299:324	living creatures surviving	299:324	Uganda is a unique region where hot areas and glaciers coexist, with a variety of living creatures surviving, but the survey on them is very poor.
32737807	9	57	theme	program	913:919	arg1	database					940:947	the program NCBI non-redundant database	909:947	the program NCBI non-redundant database	909:947	The predicted gene sequences were functionally annotated and gene prediction was carried out using the program NCBI non-redundant database.
32737807	13	58	theme	CAZyme	1399:1404	arg1	analyses					1417:1424	CAZyme annotation analyses	1399:1424	CAZyme annotation analyses	1399:1424	CAZyme annotation analyses revealed 165 genes related to carbohydrate active, and especially we have found GH1, GH2, GH3, GH38, GH35, GH51, GH51, GH53, GH106, GH146, CE12, PL1 and PL11 such as known pectin degradation genes from Sphingobium yanoikuiae.
32737807	5	59	theme	genetic	368:374	arg1	character					376:384	genetic character	368:384	genetic character	368:384	The genetic character and complete genome information of Sphingobium strains help with environmental studies and the development of better to enzyme industry.
32737807	6	60	from	OBJECTIVE	523:531	arg1	study					541:545	this study	536:545	this study	536:545	OBJECTIVE In this study, complete genome sequence of Spingobium sp.
32737807	9	61	theme	non-redundant	926:938	arg1	database					940:947	the program NCBI non-redundant database	909:947	the program NCBI non-redundant database	909:947	The predicted gene sequences were functionally annotated and gene prediction was carried out using the program NCBI non-redundant database.
32737807	3	62	theme	Uganda	209:214	arg1	glaciers					197:204	the glaciers	193:204	the glaciers of Uganda	193:214	PAMC 28499 is isolated from the glaciers of Uganda.
32737807	12	63	theme	extracellular	1355:1367	arg1	esterases					1369:1377	extracellular esterases	1355:1377	extracellular esterases	1355:1377	Its genome encodes a simple set of metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases.
32737807	2	64	theme	Spingobium	150:159	arg1	sp					161:162	BACKGROUND Spingobium sp	139:162	BACKGROUND Spingobium sp.	139:163	BACKGROUND Spingobium sp.
32737807	19	65	theme	extreme	2017:2023	arg1	environment					2025:2035	extreme environment	2017:2035	extreme environment	2017:2035	Through the complete genome we can predict how this strain can store and produce energy in extreme environment.
32737807	1	66	with	pectin	96:101	arg1	approach					129:136	comparative genomics approach	108:136	comparative genomics approach	108:136	strain PAMC 28499 reveals a potential for degrading pectin with comparative genomics approach.
32737807	3	67	attach	isolated	179:186	arg2	PAMC					165:168	PAMC 28499	165:174	PAMC 28499	165:174	PAMC 28499 is isolated from the glaciers of Uganda.
32737807	3	67	attach	isolated	179:186	arg1	glaciers					197:204	the glaciers	193:204	the glaciers of Uganda	193:214	PAMC 28499 is isolated from the glaciers of Uganda.
32737807	6	68	theme	genome	557:562	arg1	sequence					564:571	complete genome sequence	548:571	complete genome sequence of Spingobium sp	548:588	OBJECTIVE In this study, complete genome sequence of Spingobium sp.
32737807	6	68	theme	genome	557:562	arg1	OBJECTIVE					523:531	OBJECTIVE	523:531	OBJECTIVE In this study	523:545	OBJECTIVE In this study, complete genome sequence of Spingobium sp.
32737807	16	69	theme	complete	1829:1836	arg1	genome					1838:1843	the complete genome	1825:1843	the complete genome of Spingobium sp	1825:1860	CONCLUSION In this study, isolated and sequenced the complete genome of Spingobium sp.
32737807	5	70	theme	strains	433:439	arg1	character					376:384	genetic character	368:384	genetic character	368:384	The genetic character and complete genome information of Sphingobium strains help with environmental studies and the development of better to enzyme industry.
32737807	5	70	theme	strains	433:439	arg1	information					406:416	complete genome information	390:416	complete genome information	390:416	The genetic character and complete genome information of Sphingobium strains help with environmental studies and the development of better to enzyme industry.
32737807	12	71	theme	CAZymes	1342:1348	arg1	protein					1307:1313	its predicted degradation protein	1281:1313	its predicted degradation protein	1281:1313	Its genome encodes a simple set of metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases.
32737807	12	71	theme	CAZymes	1342:1348	arg1	pectin					1270:1275	pectin	1270:1275	pectin	1270:1275	Its genome encodes a simple set of metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases.
32737807	12	72	theme	degradation	1295:1305	arg1	protein					1307:1313	its predicted degradation protein	1281:1313	its predicted degradation protein	1281:1313	Its genome encodes a simple set of metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases.
32737807	8	73	theme	Genome	702:707	arg1	sequencing					709:718	METHODS Genome sequencing	694:718	METHODS Genome sequencing	694:718	METHODS Genome sequencing was performed using PacBio sequel single-molecule real-time (SMRT) sequencing technology.
32737807	15	74	contain	have	1720:1723	arg2	patterns					1733:1740	similar patterns	1725:1740	similar patterns	1725:1740	strain PAMC 28499 have similar patterns to RG I pectin-degrading pathway.
32737807	15	74	contain	have	1720:1723	arg1	28499					1714:1718	strain PAMC 28499	1702:1718	strain PAMC 28499	1702:1718	strain PAMC 28499 have similar patterns to RG I pectin-degrading pathway.
32737807	10	75	theme	carbohydrate	1050:1061	arg1	CAZymes					1079:1085	CAZymes	1079:1085	CAZymes	1079:1085	And using dbCAN2 and KEGG data base were degradation pathway predicted and protein prediction about carbohydrate active enzymes (CAZymes).
32737807	10	75	theme	carbohydrate	1050:1061	arg1	enzymes					1070:1076	carbohydrate active enzymes	1050:1076	carbohydrate active enzymes (CAZymes)	1050:1086	And using dbCAN2 and KEGG data base were degradation pathway predicted and protein prediction about carbohydrate active enzymes (CAZymes).
32737807	1	76	theme	genomics	120:127	arg1	approach					129:136	comparative genomics approach	108:136	comparative genomics approach	108:136	strain PAMC 28499 reveals a potential for degrading pectin with comparative genomics approach.
32737807	8	77	theme	sequencing	787:796	arg1	technology					798:807	PacBio sequel single-molecule real-time (SMRT) sequencing technology	740:807	PacBio sequel single-molecule real-time (SMRT) sequencing technology	740:807	METHODS Genome sequencing was performed using PacBio sequel single-molecule real-time (SMRT) sequencing technology.
32737807	11	78	theme	coding	1159:1164	arg1	content					1130:1136	64.5% GC content	1121:1136	64.5% GC content	1121:1136	RESULTS The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons.
32737807	11	78	theme	coding	1159:1164	arg1	genes					1166:1170	4432 coding protein coding genes	1139:1170	4432 coding protein coding genes	1139:1170	RESULTS The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons.
32737807	7	79	theme	Spingobium	630:639	arg1	species					641:647	Spingobium species	630:647	Spingobium species	630:647	PAMC 28499 and comparative analysis of Spingobium species strains isolated from variety of the region.
32737807	4	80	theme	unique	229:234	arg1	Uganda					217:222	Uganda	217:222	Uganda	217:222	Uganda is a unique region where hot areas and glaciers coexist, with a variety of living creatures surviving, but the survey on them is very poor.
32737807	4	80	theme	unique	229:234	arg1	region					236:241	a unique region	227:241	a unique region where hot areas and glaciers coexist	227:278	Uganda is a unique region where hot areas and glaciers coexist, with a variety of living creatures surviving, but the survey on them is very poor.
32737807	16	81	theme	sp	1859:1860	arg1	genome					1838:1843	the complete genome	1825:1843	the complete genome of Spingobium sp	1825:1860	CONCLUSION In this study, isolated and sequenced the complete genome of Spingobium sp.
32737807	8	82	theme	sequel	747:752	arg1	technology					798:807	PacBio sequel single-molecule real-time (SMRT) sequencing technology	740:807	PacBio sequel single-molecule real-time (SMRT) sequencing technology	740:807	METHODS Genome sequencing was performed using PacBio sequel single-molecule real-time (SMRT) sequencing technology.
32737807	13	83	from	yanoikuiae	1640:1649	arg1	genes					1617:1621	known pectin degradation genes	1592:1621	known pectin degradation genes from Sphingobium yanoikuiae	1592:1649	CAZyme annotation analyses revealed 165 genes related to carbohydrate active, and especially we have found GH1, GH2, GH3, GH38, GH35, GH51, GH51, GH53, GH106, GH146, CE12, PL1 and PL11 such as known pectin degradation genes from Sphingobium yanoikuiae.
32737807	20	84	theme	bioengineered	2058:2070	arg1	data					2072:2075	bioengineered data	2058:2075	bioengineered data	2058:2075	It can also provide bioengineered data by finding new genes that degradation the pectin.
32737807	13	85	theme	known	1592:1596	arg1	genes					1617:1621	known pectin degradation genes	1592:1621	known pectin degradation genes from Sphingobium yanoikuiae	1592:1649	CAZyme annotation analyses revealed 165 genes related to carbohydrate active, and especially we have found GH1, GH2, GH3, GH38, GH35, GH51, GH51, GH53, GH106, GH146, CE12, PL1 and PL11 such as known pectin degradation genes from Sphingobium yanoikuiae.
32737807	6	86	theme	sp	587:588	arg1	sequence					564:571	complete genome sequence	548:571	complete genome sequence of Spingobium sp	548:588	OBJECTIVE In this study, complete genome sequence of Spingobium sp.
32737807	6	86	theme	sp	587:588	arg1	OBJECTIVE					523:531	OBJECTIVE	523:531	OBJECTIVE In this study	523:545	OBJECTIVE In this study, complete genome sequence of Spingobium sp.
32737807	8	87	theme	real-time	770:778	arg1	technology					798:807	PacBio sequel single-molecule real-time (SMRT) sequencing technology	740:807	PacBio sequel single-molecule real-time (SMRT) sequencing technology	740:807	METHODS Genome sequencing was performed using PacBio sequel single-molecule real-time (SMRT) sequencing technology.
32737807	19	88	theme	complete	1938:1945	arg1	genome					1947:1952	the complete genome	1934:1952	the complete genome	1934:1952	Through the complete genome we can predict how this strain can store and produce energy in extreme environment.
32737807	1	89	theme	PAMC	51:54	arg1	28499					56:60	strain PAMC 28499	44:60	strain PAMC 28499	44:60	strain PAMC 28499 reveals a potential for degrading pectin with comparative genomics approach.
32737807	13	90	theme	degradation	1605:1615	arg1	genes					1617:1621	known pectin degradation genes	1592:1621	known pectin degradation genes from Sphingobium yanoikuiae	1592:1649	CAZyme annotation analyses revealed 165 genes related to carbohydrate active, and especially we have found GH1, GH2, GH3, GH38, GH35, GH51, GH51, GH53, GH106, GH146, CE12, PL1 and PL11 such as known pectin degradation genes from Sphingobium yanoikuiae.
32737807	15	91	theme	PAMC	1709:1712	arg1	28499					1714:1718	strain PAMC 28499	1702:1718	strain PAMC 28499	1702:1718	strain PAMC 28499 have similar patterns to RG I pectin-degrading pathway.
32737807	8	92	theme	SMRT	781:784	arg1	technology					798:807	PacBio sequel single-molecule real-time (SMRT) sequencing technology	740:807	PacBio sequel single-molecule real-time (SMRT) sequencing technology	740:807	METHODS Genome sequencing was performed using PacBio sequel single-molecule real-time (SMRT) sequencing technology.
32737807	7	93	theme	PAMC	591:594	arg1	28499					596:600	PAMC 28499	591:600	PAMC 28499	591:600	PAMC 28499 and comparative analysis of Spingobium species strains isolated from variety of the region.
32737807	11	94	theme	coding	1144:1149	arg1	content					1130:1136	64.5% GC content	1121:1136	64.5% GC content	1121:1136	RESULTS The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons.
32737807	11	94	theme	coding	1144:1149	arg1	genes					1166:1170	4432 coding protein coding genes	1139:1170	4432 coding protein coding genes	1139:1170	RESULTS The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons.
32737807	12	95	theme	pathways	1249:1256	arg1	set					1232:1234	a simple set	1223:1234	a simple set of metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases	1223:1396	Its genome encodes a simple set of metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases.
32737807	12	95	theme	pathways	1249:1256	arg1	pathways					1249:1256	metabolic pathways	1239:1256	metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases	1239:1396	Its genome encodes a simple set of metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases.
32737807	12	95	theme	pathways	1249:1256	arg1	genome					1208:1213	Its genome	1204:1213	Its genome	1204:1213	Its genome encodes a simple set of metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases.
32737807	9	96	theme	gene	824:827	arg1	sequences					829:837	The predicted gene sequences	810:837	The predicted gene sequences	810:837	The predicted gene sequences were functionally annotated and gene prediction was carried out using the program NCBI non-redundant database.
32737807	5	97	dep	character	376:384	arg1	The					364:366	The	364:366	The	364:366	The genetic character and complete genome information of Sphingobium strains help with environmental studies and the development of better to enzyme industry.
32737807	16	98	theme	Spingobium	1848:1857	arg1	sp					1859:1860	Spingobium sp	1848:1860	Spingobium sp	1848:1860	CONCLUSION In this study, isolated and sequenced the complete genome of Spingobium sp.
32737807	0	99	theme	genome	9:14	arg1	sequence					16:23	Complete genome sequence	0:23	Complete genome sequence of Sphingobium sp	0:41	Complete genome sequence of Sphingobium sp.
32737807	12	100	dep	pectin	1270:1275	arg1	distribution					1326:1337	an unusual distribution	1315:1337	an unusual distribution	1315:1337	Its genome encodes a simple set of metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases.
32737807	12	101	theme	simple	1225:1230	arg1	set					1232:1234	a simple set	1223:1234	a simple set of metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases	1223:1396	Its genome encodes a simple set of metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases.
32737807	12	101	theme	simple	1225:1230	arg1	pathways					1249:1256	metabolic pathways	1239:1256	metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases	1239:1396	Its genome encodes a simple set of metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases.
32737807	12	101	theme	simple	1225:1230	arg1	genome					1208:1213	Its genome	1204:1213	Its genome	1204:1213	Its genome encodes a simple set of metabolic pathways relevant to pectin and its predicted degradation protein an unusual distribution of CAZymes with extracellular esterases and pectate lyases.
32737807	11	102	contain	has	1117:1119	arg2	genes					1166:1170	4432 coding protein coding genes	1139:1170	4432 coding protein coding genes	1139:1170	RESULTS The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons.
32737807	11	102	contain	has	1117:1119	arg1	sequence					1108:1115	The genome sequence	1097:1115	The genome sequence	1097:1115	RESULTS The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons.
32737807	11	102	contain	has	1117:1119	arg2	tRNAs					1176:1180	61 tRNAs	1173:1180	61 tRNAs	1173:1180	RESULTS The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons.
32737807	11	102	contain	has	1117:1119	arg2	operons					1195:1201	12 rRNA operons	1187:1201	12 rRNA operons	1187:1201	RESULTS The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons.
32737807	11	102	contain	has	1117:1119	arg2	content					1130:1136	64.5% GC content	1121:1136	64.5% GC content	1121:1136	RESULTS The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons.
32737807	11	103	theme	64.5	1121:1124	arg1	%					1125:1125	%	1125:1125	%	1125:1125	RESULTS The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons.
32737807	16	104	dep	CONCLUSION	1776:1785	arg1	study					1795:1799	this study	1790:1799	this study	1790:1799	CONCLUSION In this study, isolated and sequenced the complete genome of Spingobium sp.
32737807	9	105	theme	gene	871:874	arg1	prediction					876:885	gene prediction	871:885	gene prediction	871:885	The predicted gene sequences were functionally annotated and gene prediction was carried out using the program NCBI non-redundant database.
32737807	14	106	theme	Sphingobium	1686:1696	arg1	sp					1698:1699	this Sphingobium sp	1681:1699	this Sphingobium sp	1681:1699	These results confirmed that this Sphingobium sp.
32737807	4	107	theme	creatures	306:314	arg1	surviving					316:324	living creatures surviving	299:324	living creatures surviving	299:324	Uganda is a unique region where hot areas and glaciers coexist, with a variety of living creatures surviving, but the survey on them is very poor.
32737807	5	108	theme	complete	390:397	arg1	information					406:416	complete genome information	390:416	complete genome information	390:416	The genetic character and complete genome information of Sphingobium strains help with environmental studies and the development of better to enzyme industry.
32737807	1	109	theme	strain	44:49	arg1	28499					56:60	strain PAMC 28499	44:60	strain PAMC 28499	44:60	strain PAMC 28499 reveals a potential for degrading pectin with comparative genomics approach.
32737807	10	110	theme	degradation	991:1001	arg1	pathway					1003:1009	degradation pathway	991:1009	degradation pathway predicted	991:1019	And using dbCAN2 and KEGG data base were degradation pathway predicted and protein prediction about carbohydrate active enzymes (CAZymes).
32737807	17	111	theme	PAMC	1863:1866	arg1	28499					1868:1872	PAMC 28499	1863:1872	PAMC 28499.	1863:1873	PAMC 28499.
32737807	11	112	theme	GC	1127:1128	arg1	genes					1166:1170	4432 coding protein coding genes	1139:1170	4432 coding protein coding genes	1139:1170	RESULTS The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons.
32737807	11	112	theme	GC	1127:1128	arg1	content					1130:1136	64.5% GC content	1121:1136	64.5% GC content	1121:1136	RESULTS The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons.
32737807	11	112	theme	GC	1127:1128	arg1	operons					1195:1201	12 rRNA operons	1187:1201	12 rRNA operons	1187:1201	RESULTS The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons.
32737807	11	112	theme	GC	1127:1128	arg1	tRNAs					1176:1180	61 tRNAs	1173:1180	61 tRNAs	1173:1180	RESULTS The genome sequence has 64.5% GC content, 4432 coding protein coding genes, 61 tRNAs, and 12 rRNA operons.
31661043	2	0	theme	Gram-stain-positive	117:135	arg1	bacterium					146:154	A Gram-stain-positive, aerobic bacterium	115:154	A Gram-stain-positive, aerobic bacterium	115:154	A Gram-stain-positive, aerobic bacterium, designated CPCC 204705T, was isolated from a desert soil sample, collected from the Badain Jaran desert.
31661043	6	1	theme	diagnostic	781:790	arg1	sugars					792:797	diagnostic sugars	781:797	diagnostic sugars	781:797	Rhamnose and galactose were detected in the whole-cell hydrolysate as diagnostic sugars.
31661043	4	2	theme	16S	543:545	arg1	rRNA					547:550	16S rRNA	543:550	16S rRNA gene sequence	543:564	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204705T belonged to the genus Cellulomonas, showing the highest similarity (98.54 %) of 16S rRNA gene sequence to Cellulomonas oligotrophica JCM 17534T.
31661043	2	3	theme	CPCC	168:171	arg1	204705T					173:179	CPCC 204705T	168:179	CPCC 204705T	168:179	A Gram-stain-positive, aerobic bacterium, designated CPCC 204705T, was isolated from a desert soil sample, collected from the Badain Jaran desert.
31661043	9	4	theme	DNA-DNA	1140:1146	arg1	hybridization					1148:1160	The DNA-DNA hybridization	1136:1160	The DNA-DNA hybridization value between strain CPCC 204705T and C. oligotrophica JCM 17534T	1136:1226	The DNA-DNA hybridization value between strain CPCC 204705T and C. oligotrophica JCM 17534T was 7.1±0.4 %, and the value of average nucleotide identity between these two strains was 79.8 %.
31661043	8	5	theme	menaquinone	902:912	arg1	H4					924:925	H4	924:925	H4	924:925	The major menaquinone was MK-9 (H4) and the polar lipid system contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, one unidentified lipid, one unidentified aminolipid and two unidentified aminophospholipids.
31661043	8	5	theme	menaquinone	902:912	arg1	MK-9					918:921	The major menaquinone was MK-9	892:921	The major menaquinone was MK-9 (H4)	892:926	The major menaquinone was MK-9 (H4) and the polar lipid system contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, one unidentified lipid, one unidentified aminolipid and two unidentified aminophospholipids.
31661043	10	6	theme	CPCC	1356:1359	arg1	204705T					1361:1367	strain CPCC 204705T	1349:1367	strain CPCC 204705T	1349:1367	The DNA G+C content of strain CPCC 204705T was 75.4 mol%.
31661043	7	7	theme	cellular	810:817	arg1	anteiso-C15 					836:847	anteiso-C15 	836:847	anteiso-C15 	836:847	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C15 : 1A, C14 : 0 and C16 : 0.
31661043	7	7	theme	cellular	810:817	arg1	acids					825:829	The major cellular fatty acids	800:829	The major cellular fatty acids	800:829	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C15 : 1A, C14 : 0 and C16 : 0.
31661043	11	8	theme	Cellulomonas	1563:1574	arg1	species					1576:1582	a novel Cellulomonas species	1555:1582	a novel Cellulomonas species	1555:1582	Based on the results of physiological experiments, chemotaxonomic data, phylogenetic analysis and DNA-DNA hybridization value, strain CPCC 204705T should be classified as a novel Cellulomonas species.
31661043	11	8	theme	Cellulomonas	1563:1574	arg1	204705T					1523:1529	strain CPCC 204705T	1511:1529	strain CPCC 204705T	1511:1529	Based on the results of physiological experiments, chemotaxonomic data, phylogenetic analysis and DNA-DNA hybridization value, strain CPCC 204705T should be classified as a novel Cellulomonas species.
31661043	1	9	attach	isolated	75:82	arg2	actinobacterium					59:73	an endoglucanase-producing actinobacterium	32:73	an endoglucanase-producing actinobacterium isolated from Badain Jaran desert sand	32:112	nov., an endoglucanase-producing actinobacterium isolated from Badain Jaran desert sand.
31661043	1	9	attach	isolated	75:82	arg1	sand					109:112	Badain Jaran desert sand	89:112	Badain Jaran desert sand	89:112	nov., an endoglucanase-producing actinobacterium isolated from Badain Jaran desert sand.
31661043	7	10	dep	 0	849:850	arg1	 0					876:877	 0	876:877	 0	876:877	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C15 : 1A, C14 : 0 and C16 : 0.
31661043	7	10	dep	 0	849:850	arg1	C14 					871:874	C14 	871:874	C14 	871:874	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C15 : 1A, C14 : 0 and C16 : 0.
31661043	7	10	dep	 0	849:850	arg1	 0					888:889	 0	888:889	 0	888:889	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C15 : 1A, C14 : 0 and C16 : 0.
31661043	7	10	dep	 0	849:850	arg1	C16 					883:886	C16 	883:886	C16 	883:886	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C15 : 1A, C14 : 0 and C16 : 0.
31661043	10	11	theme	G+C	1334:1336	arg1	%					1381:1381	75.4 mol%	1373:1381	75.4 mol%	1373:1381	The DNA G+C content of strain CPCC 204705T was 75.4 mol%.
31661043	10	11	theme	G+C	1334:1336	arg1	content					1338:1344	The DNA G+C content	1326:1344	The DNA G+C content of strain CPCC 204705T	1326:1367	The DNA G+C content of strain CPCC 204705T was 75.4 mol%.
31661043	4	12	theme	oligotrophica	582:594	arg1	17534T					600:605	Cellulomonas oligotrophica JCM 17534T	569:605	Cellulomonas oligotrophica JCM 17534T	569:605	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204705T belonged to the genus Cellulomonas, showing the highest similarity (98.54 %) of 16S rRNA gene sequence to Cellulomonas oligotrophica JCM 17534T.
31661043	4	13	theme	rRNA	409:412	arg1	sequences					419:427	16S rRNA gene sequences	405:427	16S rRNA gene sequences	405:427	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204705T belonged to the genus Cellulomonas, showing the highest similarity (98.54 %) of 16S rRNA gene sequence to Cellulomonas oligotrophica JCM 17534T.
31661043	9	14	theme	C.	1200:1201	arg1	17534T					1221:1226	C. oligotrophica JCM 17534T	1200:1226	C. oligotrophica JCM 17534T	1200:1226	The DNA-DNA hybridization value between strain CPCC 204705T and C. oligotrophica JCM 17534T was 7.1±0.4 %, and the value of average nucleotide identity between these two strains was 79.8 %.
31661043	13	15	theme	105430T=KCTC	1669:1680	arg1	204705T					1655:1661	strain CPCC 204705T	1643:1661	strain CPCC 204705T (=DSM 105430T=KCTC 39974T) as the type strain	1643:1707	nov. is proposed, with strain CPCC 204705T (=DSM 105430T=KCTC 39974T) as the type strain.
31661043	13	15	theme	105430T=KCTC	1669:1680	arg1	39974T					1682:1687	=DSM 105430T=KCTC 39974T	1664:1687	=DSM 105430T=KCTC 39974T	1664:1687	nov. is proposed, with strain CPCC 204705T (=DSM 105430T=KCTC 39974T) as the type strain.
31661043	9	16	theme	JCM	1217:1219	arg1	17534T					1221:1226	C. oligotrophica JCM 17534T	1200:1226	C. oligotrophica JCM 17534T	1200:1226	The DNA-DNA hybridization value between strain CPCC 204705T and C. oligotrophica JCM 17534T was 7.1±0.4 %, and the value of average nucleotide identity between these two strains was 79.8 %.
31661043	3	17	theme	CPCC	279:282	arg1	204705T					284:290	strain CPCC 204705T	272:290	strain CPCC 204705T	272:290	Growth of strain CPCC 204705T was observed at pH 6.0-8.0 and 15-37 °C, with optimal growth at 28 °C and pH 7.0.
31661043	11	18	theme	DNA-DNA	1482:1488	arg1	hybridization					1490:1502	DNA-DNA hybridization	1482:1502	DNA-DNA hybridization value	1482:1508	Based on the results of physiological experiments, chemotaxonomic data, phylogenetic analysis and DNA-DNA hybridization value, strain CPCC 204705T should be classified as a novel Cellulomonas species.
31661043	1	19	dep	actinobacterium	59:73	arg1	nov.					26:29	nov.	26:29	nov.	26:29	nov., an endoglucanase-producing actinobacterium isolated from Badain Jaran desert sand.
31661043	11	20	theme	experiments	1422:1432	arg1	results					1397:1403	the results	1393:1403	the results of physiological experiments, chemotaxonomic data, phylogenetic analysis and DNA-DNA hybridization value	1393:1508	Based on the results of physiological experiments, chemotaxonomic data, phylogenetic analysis and DNA-DNA hybridization value, strain CPCC 204705T should be classified as a novel Cellulomonas species.
31661043	2	21	theme	Jaran	248:252	arg1	desert					254:259	the Badain Jaran desert	237:259	the Badain Jaran desert	237:259	A Gram-stain-positive, aerobic bacterium, designated CPCC 204705T, was isolated from a desert soil sample, collected from the Badain Jaran desert.
31661043	3	22	from	pH	366:367	arg1	growth					346:351	optimal growth	338:351	optimal growth at 28 °C and pH 7.0	338:371	Growth of strain CPCC 204705T was observed at pH 6.0-8.0 and 15-37 °C, with optimal growth at 28 °C and pH 7.0.
31661043	13	23	theme	strain	1643:1648	arg1	204705T					1655:1661	strain CPCC 204705T	1643:1661	strain CPCC 204705T (=DSM 105430T=KCTC 39974T) as the type strain	1643:1707	nov. is proposed, with strain CPCC 204705T (=DSM 105430T=KCTC 39974T) as the type strain.
31661043	13	23	theme	strain	1643:1648	arg1	39974T					1682:1687	=DSM 105430T=KCTC 39974T	1664:1687	=DSM 105430T=KCTC 39974T	1664:1687	nov. is proposed, with strain CPCC 204705T (=DSM 105430T=KCTC 39974T) as the type strain.
31661043	4	24	theme	Phylogenetic	374:385	arg1	analysis					387:394	Phylogenetic analysis	374:394	Phylogenetic analysis based on 16S rRNA gene sequences	374:427	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204705T belonged to the genus Cellulomonas, showing the highest similarity (98.54 %) of 16S rRNA gene sequence to Cellulomonas oligotrophica JCM 17534T.
31661043	11	25	theme	chemotaxonomic	1435:1448	arg1	data					1450:1453	chemotaxonomic data	1435:1453	chemotaxonomic data	1435:1453	Based on the results of physiological experiments, chemotaxonomic data, phylogenetic analysis and DNA-DNA hybridization value, strain CPCC 204705T should be classified as a novel Cellulomonas species.
31661043	5	26	theme	diagnostic	687:696	arg1	acids					704:708	diagnostic amino acids	687:708	diagnostic amino acids	687:708	The peptidoglycan type was A4β, containing d-ornithine and d-glutamic acids as diagnostic amino acids.
31661043	5	26	theme	diagnostic	687:696	arg1	d-ornithine					651:661	d-ornithine	651:661	d-ornithine	651:661	The peptidoglycan type was A4β, containing d-ornithine and d-glutamic acids as diagnostic amino acids.
31661043	5	26	theme	diagnostic	687:696	arg1	acids					678:682	d-glutamic acids	667:682	d-glutamic acids	667:682	The peptidoglycan type was A4β, containing d-ornithine and d-glutamic acids as diagnostic amino acids.
31661043	8	27	theme	polar	936:940	arg1	system					948:953	the polar lipid system	932:953	the polar lipid system	932:953	The major menaquinone was MK-9 (H4) and the polar lipid system contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, one unidentified lipid, one unidentified aminolipid and two unidentified aminophospholipids.
31661043	12	28	theme	Cellulomonas	1594:1605	arg1	sp					1616:1617	The name Cellulomonas telluris sp	1585:1617	The name Cellulomonas telluris sp.	1585:1618	The name Cellulomonas telluris sp.
31661043	3	29	from	28 °C	356:360	arg1	growth					346:351	optimal growth	338:351	optimal growth at 28 °C and pH 7.0	338:371	Growth of strain CPCC 204705T was observed at pH 6.0-8.0 and 15-37 °C, with optimal growth at 28 °C and pH 7.0.
31661043	1	30	theme	Badain	89:94	arg1	sand					109:112	Badain Jaran desert sand	89:112	Badain Jaran desert sand	89:112	nov., an endoglucanase-producing actinobacterium isolated from Badain Jaran desert sand.
31661043	4	31	theme	CPCC	450:453	arg1	204705T					455:461	strain CPCC 204705T	443:461	strain CPCC 204705T	443:461	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204705T belonged to the genus Cellulomonas, showing the highest similarity (98.54 %) of 16S rRNA gene sequence to Cellulomonas oligotrophica JCM 17534T.
31661043	0	32	theme	Cellulomonas	0:11	arg1	sp					22:23	Cellulomonas telluris sp	0:23	Cellulomonas telluris sp.	0:24	Cellulomonas telluris sp.
31661043	9	33	theme	identity	1279:1286	arg1	value					1251:1255	the value	1247:1255	the value of average nucleotide identity between these two strains	1247:1312	The DNA-DNA hybridization value between strain CPCC 204705T and C. oligotrophica JCM 17534T was 7.1±0.4 %, and the value of average nucleotide identity between these two strains was 79.8 %.
31661043	9	33	theme	identity	1279:1286	arg1	%					1323:1323	79.8 %	1318:1323	79.8 %	1318:1323	The DNA-DNA hybridization value between strain CPCC 204705T and C. oligotrophica JCM 17534T was 7.1±0.4 %, and the value of average nucleotide identity between these two strains was 79.8 %.
31661043	1	34	theme	desert	102:107	arg1	sand					109:112	Badain Jaran desert sand	89:112	Badain Jaran desert sand	89:112	nov., an endoglucanase-producing actinobacterium isolated from Badain Jaran desert sand.
31661043	4	35	theme	highest	511:517	arg1	similarity					519:528	the highest similarity	507:528	the highest similarity (98.54 %) of 16S rRNA gene sequence to Cellulomonas oligotrophica JCM 17534T	507:605	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204705T belonged to the genus Cellulomonas, showing the highest similarity (98.54 %) of 16S rRNA gene sequence to Cellulomonas oligotrophica JCM 17534T.
31661043	4	35	theme	highest	511:517	arg1	%					537:537	98.54 %	531:537	98.54 %	531:537	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204705T belonged to the genus Cellulomonas, showing the highest similarity (98.54 %) of 16S rRNA gene sequence to Cellulomonas oligotrophica JCM 17534T.
31661043	6	36	theme	whole-cell	755:764	arg1	hydrolysate					766:776	the whole-cell hydrolysate	751:776	the whole-cell hydrolysate as diagnostic sugars	751:797	Rhamnose and galactose were detected in the whole-cell hydrolysate as diagnostic sugars.
31661043	2	37	attach	isolated	186:193	arg1	sample					214:219	a desert soil sample	200:219	a desert soil sample	200:219	A Gram-stain-positive, aerobic bacterium, designated CPCC 204705T, was isolated from a desert soil sample, collected from the Badain Jaran desert.
31661043	2	37	attach	isolated	186:193	arg2	bacterium					146:154	A Gram-stain-positive, aerobic bacterium	115:154	A Gram-stain-positive, aerobic bacterium	115:154	A Gram-stain-positive, aerobic bacterium, designated CPCC 204705T, was isolated from a desert soil sample, collected from the Badain Jaran desert.
31661043	8	38	theme	phosphatidylinositol	1011:1030	arg1	mannoside					1032:1040	phosphatidylinositol mannoside	1011:1040	phosphatidylinositol mannoside	1011:1040	The major menaquinone was MK-9 (H4) and the polar lipid system contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, one unidentified lipid, one unidentified aminolipid and two unidentified aminophospholipids.
31661043	5	39	contain	containing	640:649	arg2	acids					678:682	d-glutamic acids	667:682	d-glutamic acids	667:682	The peptidoglycan type was A4β, containing d-ornithine and d-glutamic acids as diagnostic amino acids.
31661043	5	39	contain	containing	640:649	arg1	type					626:629	The peptidoglycan type	608:629	The peptidoglycan type	608:629	The peptidoglycan type was A4β, containing d-ornithine and d-glutamic acids as diagnostic amino acids.
31661043	5	39	contain	containing	640:649	arg2	acids					704:708	diagnostic amino acids	687:708	diagnostic amino acids	687:708	The peptidoglycan type was A4β, containing d-ornithine and d-glutamic acids as diagnostic amino acids.
31661043	5	39	contain	containing	640:649	arg1	A4β					635:637	A4β	635:637	A4β	635:637	The peptidoglycan type was A4β, containing d-ornithine and d-glutamic acids as diagnostic amino acids.
31661043	5	39	contain	containing	640:649	arg2	d-ornithine					651:661	d-ornithine	651:661	d-ornithine	651:661	The peptidoglycan type was A4β, containing d-ornithine and d-glutamic acids as diagnostic amino acids.
31661043	7	40	dep	anteiso-C15 	836:847	arg1	 0					849:850	 0	849:850	 0	849:850	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C15 : 1A, C14 : 0 and C16 : 0.
31661043	11	41	theme	phylogenetic	1456:1467	arg1	analysis					1469:1476	phylogenetic analysis	1456:1476	phylogenetic analysis	1456:1476	Based on the results of physiological experiments, chemotaxonomic data, phylogenetic analysis and DNA-DNA hybridization value, strain CPCC 204705T should be classified as a novel Cellulomonas species.
31661043	8	42	theme	unidentified	1047:1058	arg1	lipid					1060:1064	one unidentified lipid	1043:1064	one unidentified lipid	1043:1064	The major menaquinone was MK-9 (H4) and the polar lipid system contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, one unidentified lipid, one unidentified aminolipid and two unidentified aminophospholipids.
31661043	4	43	theme	rRNA	547:550	arg1	sequence					557:564	16S rRNA gene sequence	543:564	16S rRNA gene sequence	543:564	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204705T belonged to the genus Cellulomonas, showing the highest similarity (98.54 %) of 16S rRNA gene sequence to Cellulomonas oligotrophica JCM 17534T.
31661043	11	44	theme	novel	1557:1561	arg1	species					1576:1582	a novel Cellulomonas species	1555:1582	a novel Cellulomonas species	1555:1582	Based on the results of physiological experiments, chemotaxonomic data, phylogenetic analysis and DNA-DNA hybridization value, strain CPCC 204705T should be classified as a novel Cellulomonas species.
31661043	11	44	theme	novel	1557:1561	arg1	204705T					1523:1529	strain CPCC 204705T	1511:1529	strain CPCC 204705T	1511:1529	Based on the results of physiological experiments, chemotaxonomic data, phylogenetic analysis and DNA-DNA hybridization value, strain CPCC 204705T should be classified as a novel Cellulomonas species.
31661043	1	45	theme	endoglucanase-producing	35:57	arg1	actinobacterium					59:73	an endoglucanase-producing actinobacterium	32:73	an endoglucanase-producing actinobacterium isolated from Badain Jaran desert sand	32:112	nov., an endoglucanase-producing actinobacterium isolated from Badain Jaran desert sand.
31661043	4	46	theme	sequence	557:564	arg1	similarity					519:528	the highest similarity	507:528	the highest similarity (98.54 %) of 16S rRNA gene sequence to Cellulomonas oligotrophica JCM 17534T	507:605	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204705T belonged to the genus Cellulomonas, showing the highest similarity (98.54 %) of 16S rRNA gene sequence to Cellulomonas oligotrophica JCM 17534T.
31661043	4	46	theme	sequence	557:564	arg1	%					537:537	98.54 %	531:537	98.54 %	531:537	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204705T belonged to the genus Cellulomonas, showing the highest similarity (98.54 %) of 16S rRNA gene sequence to Cellulomonas oligotrophica JCM 17534T.
31661043	8	47	theme	unidentified	1071:1082	arg1	aminolipid					1084:1093	one unidentified aminolipid	1067:1093	one unidentified aminolipid	1067:1093	The major menaquinone was MK-9 (H4) and the polar lipid system contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, one unidentified lipid, one unidentified aminolipid and two unidentified aminophospholipids.
31661043	8	48	theme	major	896:900	arg1	H4					924:925	H4	924:925	H4	924:925	The major menaquinone was MK-9 (H4) and the polar lipid system contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, one unidentified lipid, one unidentified aminolipid and two unidentified aminophospholipids.
31661043	8	48	theme	major	896:900	arg1	MK-9					918:921	The major menaquinone was MK-9	892:921	The major menaquinone was MK-9 (H4)	892:926	The major menaquinone was MK-9 (H4) and the polar lipid system contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, one unidentified lipid, one unidentified aminolipid and two unidentified aminophospholipids.
31661043	13	49	theme	type	1697:1700	arg1	strain					1702:1707	the type strain	1693:1707	the type strain	1693:1707	nov. is proposed, with strain CPCC 204705T (=DSM 105430T=KCTC 39974T) as the type strain.
31661043	11	50	theme	strain	1511:1516	arg1	204705T					1523:1529	strain CPCC 204705T	1511:1529	strain CPCC 204705T	1511:1529	Based on the results of physiological experiments, chemotaxonomic data, phylogenetic analysis and DNA-DNA hybridization value, strain CPCC 204705T should be classified as a novel Cellulomonas species.
31661043	11	50	theme	strain	1511:1516	arg1	species					1576:1582	a novel Cellulomonas species	1555:1582	a novel Cellulomonas species	1555:1582	Based on the results of physiological experiments, chemotaxonomic data, phylogenetic analysis and DNA-DNA hybridization value, strain CPCC 204705T should be classified as a novel Cellulomonas species.
31661043	5	51	theme	peptidoglycan	612:624	arg1	A4β					635:637	A4β	635:637	A4β	635:637	The peptidoglycan type was A4β, containing d-ornithine and d-glutamic acids as diagnostic amino acids.
31661043	5	51	theme	peptidoglycan	612:624	arg1	type					626:629	The peptidoglycan type	608:629	The peptidoglycan type	608:629	The peptidoglycan type was A4β, containing d-ornithine and d-glutamic acids as diagnostic amino acids.
31661043	10	52	theme	DNA	1330:1332	arg1	%					1381:1381	75.4 mol%	1373:1381	75.4 mol%	1373:1381	The DNA G+C content of strain CPCC 204705T was 75.4 mol%.
31661043	10	52	theme	DNA	1330:1332	arg1	content					1338:1344	The DNA G+C content	1326:1344	The DNA G+C content of strain CPCC 204705T	1326:1367	The DNA G+C content of strain CPCC 204705T was 75.4 mol%.
31661043	8	53	theme	was	914:916	arg1	H4					924:925	H4	924:925	H4	924:925	The major menaquinone was MK-9 (H4) and the polar lipid system contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, one unidentified lipid, one unidentified aminolipid and two unidentified aminophospholipids.
31661043	8	53	theme	was	914:916	arg1	MK-9					918:921	The major menaquinone was MK-9	892:921	The major menaquinone was MK-9 (H4)	892:926	The major menaquinone was MK-9 (H4) and the polar lipid system contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, one unidentified lipid, one unidentified aminolipid and two unidentified aminophospholipids.
31661043	11	54	theme	analysis	1469:1476	arg1	results					1397:1403	the results	1393:1403	the results of physiological experiments, chemotaxonomic data, phylogenetic analysis and DNA-DNA hybridization value	1393:1508	Based on the results of physiological experiments, chemotaxonomic data, phylogenetic analysis and DNA-DNA hybridization value, strain CPCC 204705T should be classified as a novel Cellulomonas species.
31661043	8	55	theme	unidentified	1103:1114	arg1	aminophospholipids					1116:1133	two unidentified aminophospholipids	1099:1133	two unidentified aminophospholipids	1099:1133	The major menaquinone was MK-9 (H4) and the polar lipid system contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, one unidentified lipid, one unidentified aminolipid and two unidentified aminophospholipids.
31661043	10	56	theme	strain	1349:1354	arg1	204705T					1361:1367	strain CPCC 204705T	1349:1367	strain CPCC 204705T	1349:1367	The DNA G+C content of strain CPCC 204705T was 75.4 mol%.
31661043	7	57	theme	fatty	819:823	arg1	anteiso-C15 					836:847	anteiso-C15 	836:847	anteiso-C15 	836:847	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C15 : 1A, C14 : 0 and C16 : 0.
31661043	7	57	theme	fatty	819:823	arg1	acids					825:829	The major cellular fatty acids	800:829	The major cellular fatty acids	800:829	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C15 : 1A, C14 : 0 and C16 : 0.
31661043	3	58	located	observed	296:303	arg1	15-37 °C					323:330	15-37 °C	323:330	15-37 °C	323:330	Growth of strain CPCC 204705T was observed at pH 6.0-8.0 and 15-37 °C, with optimal growth at 28 °C and pH 7.0.
31661043	3	58	located	observed	296:303	arg1	pH					308:309	pH 6.0-8.0	308:317	pH 6.0-8.0	308:317	Growth of strain CPCC 204705T was observed at pH 6.0-8.0 and 15-37 °C, with optimal growth at 28 °C and pH 7.0.
31661043	3	58	located	observed	296:303	arg2	Growth					262:267	Growth	262:267	Growth of strain CPCC 204705T	262:290	Growth of strain CPCC 204705T was observed at pH 6.0-8.0 and 15-37 °C, with optimal growth at 28 °C and pH 7.0.
31661043	9	59	theme	strain	1176:1181	arg1	204705T					1188:1194	strain CPCC 204705T	1176:1194	strain CPCC 204705T	1176:1194	The DNA-DNA hybridization value between strain CPCC 204705T and C. oligotrophica JCM 17534T was 7.1±0.4 %, and the value of average nucleotide identity between these two strains was 79.8 %.
31661043	9	60	theme	CPCC	1183:1186	arg1	204705T					1188:1194	strain CPCC 204705T	1176:1194	strain CPCC 204705T	1176:1194	The DNA-DNA hybridization value between strain CPCC 204705T and C. oligotrophica JCM 17534T was 7.1±0.4 %, and the value of average nucleotide identity between these two strains was 79.8 %.
31661043	7	61	theme	major	804:808	arg1	anteiso-C15 					836:847	anteiso-C15 	836:847	anteiso-C15 	836:847	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C15 : 1A, C14 : 0 and C16 : 0.
31661043	7	61	theme	major	804:808	arg1	acids					825:829	The major cellular fatty acids	800:829	The major cellular fatty acids	800:829	The major cellular fatty acids were anteiso-C15 : 0, anteiso-C15 : 1A, C14 : 0 and C16 : 0.
31661043	3	62	theme	strain	272:277	arg1	204705T					284:290	strain CPCC 204705T	272:290	strain CPCC 204705T	272:290	Growth of strain CPCC 204705T was observed at pH 6.0-8.0 and 15-37 °C, with optimal growth at 28 °C and pH 7.0.
31661043	4	63	theme	gene	414:417	arg1	sequences					419:427	16S rRNA gene sequences	405:427	16S rRNA gene sequences	405:427	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204705T belonged to the genus Cellulomonas, showing the highest similarity (98.54 %) of 16S rRNA gene sequence to Cellulomonas oligotrophica JCM 17534T.
31661043	4	64	theme	Cellulomonas	569:580	arg1	17534T					600:605	Cellulomonas oligotrophica JCM 17534T	569:605	Cellulomonas oligotrophica JCM 17534T	569:605	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204705T belonged to the genus Cellulomonas, showing the highest similarity (98.54 %) of 16S rRNA gene sequence to Cellulomonas oligotrophica JCM 17534T.
31661043	5	65	theme	d-glutamic	667:676	arg1	acids					704:708	diagnostic amino acids	687:708	diagnostic amino acids	687:708	The peptidoglycan type was A4β, containing d-ornithine and d-glutamic acids as diagnostic amino acids.
31661043	5	65	theme	d-glutamic	667:676	arg1	d-ornithine					651:661	d-ornithine	651:661	d-ornithine	651:661	The peptidoglycan type was A4β, containing d-ornithine and d-glutamic acids as diagnostic amino acids.
31661043	5	65	theme	d-glutamic	667:676	arg1	acids					678:682	d-glutamic acids	667:682	d-glutamic acids	667:682	The peptidoglycan type was A4β, containing d-ornithine and d-glutamic acids as diagnostic amino acids.
31661043	11	66	theme	hybridization	1490:1502	arg1	value					1504:1508	DNA-DNA hybridization value	1482:1508	DNA-DNA hybridization value	1482:1508	Based on the results of physiological experiments, chemotaxonomic data, phylogenetic analysis and DNA-DNA hybridization value, strain CPCC 204705T should be classified as a novel Cellulomonas species.
31661043	4	67	theme	JCM	596:598	arg1	17534T					600:605	Cellulomonas oligotrophica JCM 17534T	569:605	Cellulomonas oligotrophica JCM 17534T	569:605	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204705T belonged to the genus Cellulomonas, showing the highest similarity (98.54 %) of 16S rRNA gene sequence to Cellulomonas oligotrophica JCM 17534T.
31661043	13	68	theme	=DSM	1664:1667	arg1	204705T					1655:1661	strain CPCC 204705T	1643:1661	strain CPCC 204705T (=DSM 105430T=KCTC 39974T) as the type strain	1643:1707	nov. is proposed, with strain CPCC 204705T (=DSM 105430T=KCTC 39974T) as the type strain.
31661043	13	68	theme	=DSM	1664:1667	arg1	39974T					1682:1687	=DSM 105430T=KCTC 39974T	1664:1687	=DSM 105430T=KCTC 39974T	1664:1687	nov. is proposed, with strain CPCC 204705T (=DSM 105430T=KCTC 39974T) as the type strain.
31661043	2	69	theme	desert	202:207	arg1	sample					214:219	a desert soil sample	200:219	a desert soil sample	200:219	A Gram-stain-positive, aerobic bacterium, designated CPCC 204705T, was isolated from a desert soil sample, collected from the Badain Jaran desert.
31661043	11	70	theme	physiological	1408:1420	arg1	experiments					1422:1432	physiological experiments	1408:1432	physiological experiments	1408:1432	Based on the results of physiological experiments, chemotaxonomic data, phylogenetic analysis and DNA-DNA hybridization value, strain CPCC 204705T should be classified as a novel Cellulomonas species.
31661043	11	71	theme	value	1504:1508	arg1	results					1397:1403	the results	1393:1403	the results of physiological experiments, chemotaxonomic data, phylogenetic analysis and DNA-DNA hybridization value	1393:1508	Based on the results of physiological experiments, chemotaxonomic data, phylogenetic analysis and DNA-DNA hybridization value, strain CPCC 204705T should be classified as a novel Cellulomonas species.
31661043	3	72	theme	204705T	284:290	arg1	Growth					262:267	Growth	262:267	Growth of strain CPCC 204705T	262:290	Growth of strain CPCC 204705T was observed at pH 6.0-8.0 and 15-37 °C, with optimal growth at 28 °C and pH 7.0.
31661043	4	73	theme	16S	405:407	arg1	sequences					419:427	16S rRNA gene sequences	405:427	16S rRNA gene sequences	405:427	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204705T belonged to the genus Cellulomonas, showing the highest similarity (98.54 %) of 16S rRNA gene sequence to Cellulomonas oligotrophica JCM 17534T.
31661043	2	74	theme	Badain	241:246	arg1	desert					254:259	the Badain Jaran desert	237:259	the Badain Jaran desert	237:259	A Gram-stain-positive, aerobic bacterium, designated CPCC 204705T, was isolated from a desert soil sample, collected from the Badain Jaran desert.
31661043	4	75	theme	gene	552:555	arg1	sequence					557:564	16S rRNA gene sequence	543:564	16S rRNA gene sequence	543:564	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204705T belonged to the genus Cellulomonas, showing the highest similarity (98.54 %) of 16S rRNA gene sequence to Cellulomonas oligotrophica JCM 17534T.
31661043	11	76	theme	data	1450:1453	arg1	results					1397:1403	the results	1393:1403	the results of physiological experiments, chemotaxonomic data, phylogenetic analysis and DNA-DNA hybridization value	1393:1508	Based on the results of physiological experiments, chemotaxonomic data, phylogenetic analysis and DNA-DNA hybridization value, strain CPCC 204705T should be classified as a novel Cellulomonas species.
31661043	6	77	located	detected	739:746	arg2	galactose					724:732	galactose	724:732	galactose	724:732	Rhamnose and galactose were detected in the whole-cell hydrolysate as diagnostic sugars.
31661043	6	77	located	detected	739:746	arg1	hydrolysate					766:776	the whole-cell hydrolysate	751:776	the whole-cell hydrolysate as diagnostic sugars	751:797	Rhamnose and galactose were detected in the whole-cell hydrolysate as diagnostic sugars.
31661043	6	77	located	detected	739:746	arg2	Rhamnose					711:718	Rhamnose	711:718	Rhamnose	711:718	Rhamnose and galactose were detected in the whole-cell hydrolysate as diagnostic sugars.
31661043	4	78	theme	genus	479:483	arg1	Cellulomonas					485:496	the genus Cellulomonas	475:496	the genus Cellulomonas	475:496	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204705T belonged to the genus Cellulomonas, showing the highest similarity (98.54 %) of 16S rRNA gene sequence to Cellulomonas oligotrophica JCM 17534T.
31661043	10	79	theme	75.4 mol	1373:1380	arg1	%					1381:1381	75.4 mol%	1373:1381	75.4 mol%	1373:1381	The DNA G+C content of strain CPCC 204705T was 75.4 mol%.
31661043	10	79	theme	75.4 mol	1373:1380	arg1	content					1338:1344	The DNA G+C content	1326:1344	The DNA G+C content of strain CPCC 204705T	1326:1367	The DNA G+C content of strain CPCC 204705T was 75.4 mol%.
31661043	13	80	theme	CPCC	1650:1653	arg1	204705T					1655:1661	strain CPCC 204705T	1643:1661	strain CPCC 204705T (=DSM 105430T=KCTC 39974T) as the type strain	1643:1707	nov. is proposed, with strain CPCC 204705T (=DSM 105430T=KCTC 39974T) as the type strain.
31661043	13	80	theme	CPCC	1650:1653	arg1	39974T					1682:1687	=DSM 105430T=KCTC 39974T	1664:1687	=DSM 105430T=KCTC 39974T	1664:1687	nov. is proposed, with strain CPCC 204705T (=DSM 105430T=KCTC 39974T) as the type strain.
31661043	8	81	theme	lipid	942:946	arg1	system					948:953	the polar lipid system	932:953	the polar lipid system	932:953	The major menaquinone was MK-9 (H4) and the polar lipid system contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, one unidentified lipid, one unidentified aminolipid and two unidentified aminophospholipids.
31661043	3	82	theme	optimal	338:344	arg1	growth					346:351	optimal growth	338:351	optimal growth at 28 °C and pH 7.0	338:371	Growth of strain CPCC 204705T was observed at pH 6.0-8.0 and 15-37 °C, with optimal growth at 28 °C and pH 7.0.
31661043	4	83	theme	strain	443:448	arg1	204705T					455:461	strain CPCC 204705T	443:461	strain CPCC 204705T	443:461	Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CPCC 204705T belonged to the genus Cellulomonas, showing the highest similarity (98.54 %) of 16S rRNA gene sequence to Cellulomonas oligotrophica JCM 17534T.
31661043	10	84	theme	204705T	1361:1367	arg1	%					1381:1381	75.4 mol%	1373:1381	75.4 mol%	1373:1381	The DNA G+C content of strain CPCC 204705T was 75.4 mol%.
31661043	10	84	theme	204705T	1361:1367	arg1	content					1338:1344	The DNA G+C content	1326:1344	The DNA G+C content of strain CPCC 204705T	1326:1367	The DNA G+C content of strain CPCC 204705T was 75.4 mol%.
31661043	5	85	theme	amino	698:702	arg1	acids					704:708	diagnostic amino acids	687:708	diagnostic amino acids	687:708	The peptidoglycan type was A4β, containing d-ornithine and d-glutamic acids as diagnostic amino acids.
31661043	5	85	theme	amino	698:702	arg1	d-ornithine					651:661	d-ornithine	651:661	d-ornithine	651:661	The peptidoglycan type was A4β, containing d-ornithine and d-glutamic acids as diagnostic amino acids.
31661043	5	85	theme	amino	698:702	arg1	acids					678:682	d-glutamic acids	667:682	d-glutamic acids	667:682	The peptidoglycan type was A4β, containing d-ornithine and d-glutamic acids as diagnostic amino acids.
31661043	12	86	theme	name	1589:1592	arg1	sp					1616:1617	The name Cellulomonas telluris sp	1585:1617	The name Cellulomonas telluris sp.	1585:1618	The name Cellulomonas telluris sp.
31661043	0	87	theme	telluris	13:20	arg1	sp					22:23	Cellulomonas telluris sp	0:23	Cellulomonas telluris sp.	0:24	Cellulomonas telluris sp.
31661043	9	88	theme	nucleotide	1268:1277	arg1	identity					1279:1286	average nucleotide identity	1260:1286	average nucleotide identity between these two strains	1260:1312	The DNA-DNA hybridization value between strain CPCC 204705T and C. oligotrophica JCM 17534T was 7.1±0.4 %, and the value of average nucleotide identity between these two strains was 79.8 %.
31661043	1	89	theme	Jaran	96:100	arg1	sand					109:112	Badain Jaran desert sand	89:112	Badain Jaran desert sand	89:112	nov., an endoglucanase-producing actinobacterium isolated from Badain Jaran desert sand.
31661043	12	90	theme	telluris	1607:1614	arg1	sp					1616:1617	The name Cellulomonas telluris sp	1585:1617	The name Cellulomonas telluris sp.	1585:1618	The name Cellulomonas telluris sp.
31661043	8	91	contain	contained	955:963	arg2	phosphatidylglycerol					989:1008	phosphatidylglycerol	989:1008	phosphatidylglycerol	989:1008	The major menaquinone was MK-9 (H4) and the polar lipid system contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, one unidentified lipid, one unidentified aminolipid and two unidentified aminophospholipids.
31661043	8	91	contain	contained	955:963	arg2	mannoside					1032:1040	phosphatidylinositol mannoside	1011:1040	phosphatidylinositol mannoside	1011:1040	The major menaquinone was MK-9 (H4) and the polar lipid system contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, one unidentified lipid, one unidentified aminolipid and two unidentified aminophospholipids.
31661043	8	91	contain	contained	955:963	arg2	diphosphatidylglycerol					965:986	diphosphatidylglycerol	965:986	diphosphatidylglycerol	965:986	The major menaquinone was MK-9 (H4) and the polar lipid system contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, one unidentified lipid, one unidentified aminolipid and two unidentified aminophospholipids.
31661043	8	91	contain	contained	955:963	arg2	aminolipid					1084:1093	one unidentified aminolipid	1067:1093	one unidentified aminolipid	1067:1093	The major menaquinone was MK-9 (H4) and the polar lipid system contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, one unidentified lipid, one unidentified aminolipid and two unidentified aminophospholipids.
31661043	8	91	contain	contained	955:963	arg1	MK-9					918:921	The major menaquinone was MK-9	892:921	The major menaquinone was MK-9 (H4)	892:926	The major menaquinone was MK-9 (H4) and the polar lipid system contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, one unidentified lipid, one unidentified aminolipid and two unidentified aminophospholipids.
31661043	8	91	contain	contained	955:963	arg2	lipid					1060:1064	one unidentified lipid	1043:1064	one unidentified lipid	1043:1064	The major menaquinone was MK-9 (H4) and the polar lipid system contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, one unidentified lipid, one unidentified aminolipid and two unidentified aminophospholipids.
31661043	8	91	contain	contained	955:963	arg1	H4					924:925	H4	924:925	H4	924:925	The major menaquinone was MK-9 (H4) and the polar lipid system contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, one unidentified lipid, one unidentified aminolipid and two unidentified aminophospholipids.
31661043	8	91	contain	contained	955:963	arg1	system					948:953	the polar lipid system	932:953	the polar lipid system	932:953	The major menaquinone was MK-9 (H4) and the polar lipid system contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, one unidentified lipid, one unidentified aminolipid and two unidentified aminophospholipids.
31661043	8	91	contain	contained	955:963	arg2	aminophospholipids					1116:1133	two unidentified aminophospholipids	1099:1133	two unidentified aminophospholipids	1099:1133	The major menaquinone was MK-9 (H4) and the polar lipid system contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside, one unidentified lipid, one unidentified aminolipid and two unidentified aminophospholipids.
31661043	2	92	dep	Gram-stain-positive	117:135	arg1	aerobic					138:144	aerobic	138:144	aerobic	138:144	A Gram-stain-positive, aerobic bacterium, designated CPCC 204705T, was isolated from a desert soil sample, collected from the Badain Jaran desert.
31661043	9	93	theme	average	1260:1266	arg1	identity					1279:1286	average nucleotide identity	1260:1286	average nucleotide identity between these two strains	1260:1312	The DNA-DNA hybridization value between strain CPCC 204705T and C. oligotrophica JCM 17534T was 7.1±0.4 %, and the value of average nucleotide identity between these two strains was 79.8 %.
31661043	2	94	theme	soil	209:212	arg1	sample					214:219	a desert soil sample	200:219	a desert soil sample	200:219	A Gram-stain-positive, aerobic bacterium, designated CPCC 204705T, was isolated from a desert soil sample, collected from the Badain Jaran desert.
31661043	11	95	theme	CPCC	1518:1521	arg1	204705T					1523:1529	strain CPCC 204705T	1511:1529	strain CPCC 204705T	1511:1529	Based on the results of physiological experiments, chemotaxonomic data, phylogenetic analysis and DNA-DNA hybridization value, strain CPCC 204705T should be classified as a novel Cellulomonas species.
31661043	11	95	theme	CPCC	1518:1521	arg1	species					1576:1582	a novel Cellulomonas species	1555:1582	a novel Cellulomonas species	1555:1582	Based on the results of physiological experiments, chemotaxonomic data, phylogenetic analysis and DNA-DNA hybridization value, strain CPCC 204705T should be classified as a novel Cellulomonas species.
31661043	9	96	dep	C.	1200:1201	arg1	oligotrophica					1203:1215	oligotrophica	1203:1215	oligotrophica	1203:1215	The DNA-DNA hybridization value between strain CPCC 204705T and C. oligotrophica JCM 17534T was 7.1±0.4 %, and the value of average nucleotide identity between these two strains was 79.8 %.
31661043	9	97	theme	hybridization	1148:1160	arg1	value					1162:1166	The DNA-DNA hybridization value	1136:1166	The DNA-DNA hybridization value between strain CPCC 204705T and C. oligotrophica JCM 17534T	1136:1226	The DNA-DNA hybridization value between strain CPCC 204705T and C. oligotrophica JCM 17534T was 7.1±0.4 %, and the value of average nucleotide identity between these two strains was 79.8 %.
34883218	12	0	theme	inhibitory	1876:1885	arg1	effect					1887:1892	the most significant inhibitory effect	1855:1892	the most significant inhibitory effect on the production of NO (the inhibitory is 89%) induced by LPS	1855:1955	RESULTS In preliminary anti-inflammatory screening, AIDE showed the lowest cytotoxicity and the most significant inhibitory effect on the production of NO (the inhibitory is 89%) induced by LPS among the tested five extracts.
34883218	4	1	theme	STUDY	336:340	arg1	AIM					325:327	AIM	325:327	AIM OF THE STUDY	325:340	AIM OF THE STUDY To clarify the anti-inflammatory activity of A. italica, to reveal its molecular mechanisms, and to discover the anti-inflammatory active ingredients.
34883218	7	2	theme	chemical	1133:1140	arg1	composition					1142:1152	the chemical composition	1129:1152	the chemical composition of AIDE	1129:1160	LC-MS/MS experiment was applied to analyze the chemical composition of AIDE.
34883218	2	3	dep	Retz	194:197	arg1	RELEVANCE					168:176	ETHNOPHARMACOLOGICAL RELEVANCE	147:176	ETHNOPHARMACOLOGICAL RELEVANCE	147:176	ETHNOPHARMACOLOGICAL RELEVANCE Anchusa italica Retz.
34883218	14	4	theme	signal-regulated	2218:2233	arg1	ERK					2251:2253	ERK	2251:2253	ERK	2251:2253	AIDE could inhibit LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α and down-regulate the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation.
34883218	14	4	theme	signal-regulated	2218:2233	arg1	kinase					2243:2248	extracellular signal-regulated protein kinase	2204:2248	extracellular signal-regulated protein kinase (ERK)	2204:2254	AIDE could inhibit LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α and down-regulate the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation.
34883218	8	5	from	levels	1253:1258	arg1	supernatants					1271:1282	culture supernatants	1263:1282	culture supernatants	1263:1282	MTT and Griess methods were used to detect the cell viability and to quantify the nitrite levels in culture supernatants, respectively.
34883218	9	6	theme	E2	1313:1314	arg1	production					1405:1414	Prostaglandin E2 (PGE2), interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α) production	1299:1414	Prostaglandin E2 (PGE2), interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α) production	1299:1414	Prostaglandin E2 (PGE2), interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α) production was examined by ELISA assays.
34883218	12	7	theme	lowest	1831:1836	arg1	cytotoxicity					1838:1849	cytotoxicity	1838:1849	cytotoxicity	1838:1849	RESULTS In preliminary anti-inflammatory screening, AIDE showed the lowest cytotoxicity and the most significant inhibitory effect on the production of NO (the inhibitory is 89%) induced by LPS among the tested five extracts.
34883218	6	8	theme	AIPE	825:828	arg1	effects					814:820	the effects	810:820	the effects of AIPE, AIDE, AIEE, AIBE and AIW on cell viability and nitric oxide (NO)	810:894	By measuring the effects of AIPE, AIDE, AIEE, AIBE and AIW on cell viability and nitric oxide (NO) in Lipopolysaccharide (LPS) stimulated RAW264.7 cell lines, AIDE with the lowest cytotoxicity and NO contents was finally selected for further chemical and anti-inflammatory investigations.
34883218	3	9	theme	important	221:229	arg1	plant					241:245	an important medicinal plant	218:245	an important medicinal plant for the treatment of meningitis and pneumonia in traditional Uygur medicines	218:322	(Boraginaceae) is an important medicinal plant for the treatment of meningitis and pneumonia in traditional Uygur medicines.
34883218	17	10	theme	pyrin	2740:2744	arg1	expression					2674:2683	the protein expression	2662:2683	the protein expression of pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3 (NLRP3) and IL-1β	2662:2782	In addition, the protein expression of pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3 (NLRP3) and IL-1β were decreased after the AIDE treatment.
34883218	16	11	theme	P65	2563:2565	arg1	translocation					2575:2587	P65 nuclear translocation	2563:2587	P65 nuclear translocation	2563:2587	After the treatment of AIDE, P65 nuclear translocation was inhibited and Nrf2 nuclear translocation was increased.
34883218	19	12	theme	anti-inflammatory	2871:2887	arg1	activity					2889:2896	its anti-inflammatory activity	2867:2896	its anti-inflammatory activity	2867:2896	exerted its anti-inflammatory activity by inhibiting the mitogen-activated protein kinase (MAPK), nuclear transcription factors κB (NF-κB) and pyrolytic relevant proteins, down-regulating inflammatory factor levels, and activating the Nrf2/HO-1 pathway.
34883218	17	13	theme	IL-1β	2778:2782	arg1	expression					2674:2683	the protein expression	2662:2683	the protein expression of pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3 (NLRP3) and IL-1β	2662:2782	In addition, the protein expression of pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3 (NLRP3) and IL-1β were decreased after the AIDE treatment.
34883218	15	14	theme	GSTA1	2421:2425	arg1	level					2464:2468	the protein expression level	2441:2468	the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1	2441:2531	It promoted the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1 and the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1.
34883218	15	14	theme	GSTA1	2421:2425	arg1	level					2399:2403	the mRNA expression level	2379:2403	the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1	2379:2435	It promoted the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1 and the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1.
34883218	12	15	from	cytotoxicity	1838:1849	arg1	production					1901:1910	the production	1897:1910	the production of NO (the inhibitory is 89%) induced by LPS	1897:1955	RESULTS In preliminary anti-inflammatory screening, AIDE showed the lowest cytotoxicity and the most significant inhibitory effect on the production of NO (the inhibitory is 89%) induced by LPS among the tested five extracts.
34883218	6	16	from	effects	814:820	arg1	oxide					885:889	nitric oxide	878:889	nitric oxide (NO)	878:894	By measuring the effects of AIPE, AIDE, AIEE, AIBE and AIW on cell viability and nitric oxide (NO) in Lipopolysaccharide (LPS) stimulated RAW264.7 cell lines, AIDE with the lowest cytotoxicity and NO contents was finally selected for further chemical and anti-inflammatory investigations.
34883218	6	16	from	effects	814:820	arg1	NO					892:893	NO	892:893	NO	892:893	By measuring the effects of AIPE, AIDE, AIEE, AIBE and AIW on cell viability and nitric oxide (NO) in Lipopolysaccharide (LPS) stimulated RAW264.7 cell lines, AIDE with the lowest cytotoxicity and NO contents was finally selected for further chemical and anti-inflammatory investigations.
34883218	6	16	from	effects	814:820	arg1	viability					864:872	cell viability	859:872	cell viability	859:872	By measuring the effects of AIPE, AIDE, AIEE, AIBE and AIW on cell viability and nitric oxide (NO) in Lipopolysaccharide (LPS) stimulated RAW264.7 cell lines, AIDE with the lowest cytotoxicity and NO contents was finally selected for further chemical and anti-inflammatory investigations.
34883218	2	17	theme	ETHNOPHARMACOLOGICAL	147:166	arg1	RELEVANCE					168:176	ETHNOPHARMACOLOGICAL RELEVANCE	147:176	ETHNOPHARMACOLOGICAL RELEVANCE	147:176	ETHNOPHARMACOLOGICAL RELEVANCE Anchusa italica Retz.
34883218	19	18	theme	protein	2934:2940	arg1	MAPK					2950:2953	MAPK	2950:2953	MAPK	2950:2953	exerted its anti-inflammatory activity by inhibiting the mitogen-activated protein kinase (MAPK), nuclear transcription factors κB (NF-κB) and pyrolytic relevant proteins, down-regulating inflammatory factor levels, and activating the Nrf2/HO-1 pathway.
34883218	19	18	theme	protein	2934:2940	arg1	kinase					2942:2947	the mitogen-activated protein kinase	2912:2947	the mitogen-activated protein kinase (MAPK)	2912:2954	exerted its anti-inflammatory activity by inhibiting the mitogen-activated protein kinase (MAPK), nuclear transcription factors κB (NF-κB) and pyrolytic relevant proteins, down-regulating inflammatory factor levels, and activating the Nrf2/HO-1 pathway.
34883218	15	19	theme	NQO-1	2414:2418	arg1	level					2464:2468	the protein expression level	2441:2468	the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1	2441:2531	It promoted the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1 and the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1.
34883218	15	19	theme	NQO-1	2414:2418	arg1	level					2399:2403	the mRNA expression level	2379:2403	the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1	2379:2435	It promoted the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1 and the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1.
34883218	14	20	theme	kinase	2274:2279	arg1	levels					2194:2199	the levels	2190:2199	the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation	2190:2364	AIDE could inhibit LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α and down-regulate the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation.
34883218	6	21	theme	further	1031:1037	arg1	investigations					1070:1083	further chemical and anti-inflammatory investigations	1031:1083	further chemical and anti-inflammatory investigations	1031:1083	By measuring the effects of AIPE, AIDE, AIEE, AIBE and AIW on cell viability and nitric oxide (NO) in Lipopolysaccharide (LPS) stimulated RAW264.7 cell lines, AIDE with the lowest cytotoxicity and NO contents was finally selected for further chemical and anti-inflammatory investigations.
34883218	10	22	theme	Real-time	1446:1454	arg1	PCR					1469:1471	Real-time quantitative PCR	1446:1471	Real-time quantitative PCR	1446:1471	Real-time quantitative PCR was used to detect the expression of hemeoxygenase-1 (HO-1), Nrf2-mediated quinone oxidoreductase 1 (NQO-1), glutathione S-transferase A 1 (GSTA1) and glutathione S-transferase M 1 (GSTM1) mRNA.
34883218	19	23	theme	factor	3060:3065	arg1	levels					3067:3072	inflammatory factor levels	3047:3072	inflammatory factor levels	3047:3072	exerted its anti-inflammatory activity by inhibiting the mitogen-activated protein kinase (MAPK), nuclear transcription factors κB (NF-κB) and pyrolytic relevant proteins, down-regulating inflammatory factor levels, and activating the Nrf2/HO-1 pathway.
34883218	15	24	theme	HO-1	2528:2531	arg1	level					2464:2468	the protein expression level	2441:2468	the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1	2441:2531	It promoted the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1 and the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1.
34883218	15	24	theme	HO-1	2528:2531	arg1	level					2399:2403	the mRNA expression level	2379:2403	the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1	2379:2435	It promoted the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1 and the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1.
34883218	15	25	theme	HO-1	2408:2411	arg1	level					2464:2468	the protein expression level	2441:2468	the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1	2441:2531	It promoted the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1 and the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1.
34883218	15	25	theme	HO-1	2408:2411	arg1	level					2399:2403	the mRNA expression level	2379:2403	the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1	2379:2435	It promoted the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1 and the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1.
34883218	6	26	theme	cell	859:862	arg1	viability					864:872	cell viability	859:872	cell viability	859:872	By measuring the effects of AIPE, AIDE, AIEE, AIBE and AIW on cell viability and nitric oxide (NO) in Lipopolysaccharide (LPS) stimulated RAW264.7 cell lines, AIDE with the lowest cytotoxicity and NO contents was finally selected for further chemical and anti-inflammatory investigations.
34883218	4	27	theme	italica	390:396	arg1	activity					375:382	the anti-inflammatory activity	353:382	the anti-inflammatory activity of A. italica	353:396	AIM OF THE STUDY To clarify the anti-inflammatory activity of A. italica, to reveal its molecular mechanisms, and to discover the anti-inflammatory active ingredients.
34883218	16	28	theme	Nrf2	2607:2610	arg1	translocation					2620:2632	Nrf2 nuclear translocation	2607:2632	Nrf2 nuclear translocation	2607:2632	After the treatment of AIDE, P65 nuclear translocation was inhibited and Nrf2 nuclear translocation was increased.
34883218	8	29	theme	MTT	1163:1165	arg1	methods					1178:1184	MTT and Griess methods	1163:1184	MTT and Griess methods	1163:1184	MTT and Griess methods were used to detect the cell viability and to quantify the nitrite levels in culture supernatants, respectively.
34883218	16	30	theme	nuclear	2612:2618	arg1	translocation					2620:2632	Nrf2 nuclear translocation	2607:2632	Nrf2 nuclear translocation	2607:2632	After the treatment of AIDE, P65 nuclear translocation was inhibited and Nrf2 nuclear translocation was increased.
34883218	10	31	used	used	1477:1480	arg2	PCR					1469:1471	Real-time quantitative PCR	1446:1471	Real-time quantitative PCR	1446:1471	Real-time quantitative PCR was used to detect the expression of hemeoxygenase-1 (HO-1), Nrf2-mediated quinone oxidoreductase 1 (NQO-1), glutathione S-transferase A 1 (GSTA1) and glutathione S-transferase M 1 (GSTM1) mRNA.
34883218	8	32	theme	Griess	1171:1176	arg1	methods					1178:1184	MTT and Griess methods	1163:1184	MTT and Griess methods	1163:1184	MTT and Griess methods were used to detect the cell viability and to quantify the nitrite levels in culture supernatants, respectively.
34883218	5	33	theme	dichloromethane	690:704	arg1	AIDE					715:718	AIDE	715:718	AIDE	715:718	MATERIALS AND METHODS Dried and crushed aerial parts of A. italica were extracted with 75% ethanol to yield crude extract (AICE) and AICE was fractionated to obtain petroleum ether extract (AIPE), dichloromethane extract (AIDE), ethyl acetate extract (AIEE), n-butanol extract (AIBE) and residues (AIW).
34883218	5	33	theme	dichloromethane	690:704	arg1	extract					706:712	dichloromethane extract	690:712	dichloromethane extract (AIDE)	690:719	MATERIALS AND METHODS Dried and crushed aerial parts of A. italica were extracted with 75% ethanol to yield crude extract (AICE) and AICE was fractionated to obtain petroleum ether extract (AIPE), dichloromethane extract (AIDE), ethyl acetate extract (AIEE), n-butanol extract (AIBE) and residues (AIW).
34883218	17	34	theme	family	2733:2738	arg1	pyrin					2740:2744	pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3	2688:2764	pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3 (NLRP3)	2688:2772	In addition, the protein expression of pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3 (NLRP3) and IL-1β were decreased after the AIDE treatment.
34883218	17	34	theme	family	2733:2738	arg1	NLRP3					2767:2771	NLRP3	2767:2771	NLRP3	2767:2771	In addition, the protein expression of pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3 (NLRP3) and IL-1β were decreased after the AIDE treatment.
34883218	5	35	theme	italica	552:558	arg1	parts					540:544	aerial parts	533:544	aerial parts of A. italica	533:558	MATERIALS AND METHODS Dried and crushed aerial parts of A. italica were extracted with 75% ethanol to yield crude extract (AICE) and AICE was fractionated to obtain petroleum ether extract (AIPE), dichloromethane extract (AIDE), ethyl acetate extract (AIEE), n-butanol extract (AIBE) and residues (AIW).
34883218	9	36	theme	interleukin-1β	1346:1359	arg1	production					1405:1414	Prostaglandin E2 (PGE2), interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α) production	1299:1414	Prostaglandin E2 (PGE2), interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α) production	1299:1414	Prostaglandin E2 (PGE2), interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α) production was examined by ELISA assays.
34883218	12	37	theme	anti-inflammatory	1786:1802	arg1	AIDE					1815:1818	AIDE	1815:1818	AIDE	1815:1818	RESULTS In preliminary anti-inflammatory screening, AIDE showed the lowest cytotoxicity and the most significant inhibitory effect on the production of NO (the inhibitory is 89%) induced by LPS among the tested five extracts.
34883218	12	37	theme	anti-inflammatory	1786:1802	arg1	screening					1804:1812	preliminary anti-inflammatory screening	1774:1812	preliminary anti-inflammatory screening	1774:1812	RESULTS In preliminary anti-inflammatory screening, AIDE showed the lowest cytotoxicity and the most significant inhibitory effect on the production of NO (the inhibitory is 89%) induced by LPS among the tested five extracts.
34883218	4	38	theme	anti-inflammatory	455:471	arg1	ingredients					480:490	the anti-inflammatory active ingredients	451:490	the anti-inflammatory active ingredients	451:490	AIM OF THE STUDY To clarify the anti-inflammatory activity of A. italica, to reveal its molecular mechanisms, and to discover the anti-inflammatory active ingredients.
34883218	12	39	theme	NO	1915:1916	arg1	production					1901:1910	the production	1897:1910	the production of NO (the inhibitory is 89%) induced by LPS	1897:1955	RESULTS In preliminary anti-inflammatory screening, AIDE showed the lowest cytotoxicity and the most significant inhibitory effect on the production of NO (the inhibitory is 89%) induced by LPS among the tested five extracts.
34883218	5	40	theme	ether	668:672	arg1	extract					674:680	petroleum ether extract	658:680	petroleum ether extract (AIPE)	658:687	MATERIALS AND METHODS Dried and crushed aerial parts of A. italica were extracted with 75% ethanol to yield crude extract (AICE) and AICE was fractionated to obtain petroleum ether extract (AIPE), dichloromethane extract (AIDE), ethyl acetate extract (AIEE), n-butanol extract (AIBE) and residues (AIW).
34883218	5	40	theme	ether	668:672	arg1	AIPE					683:686	AIPE	683:686	AIPE	683:686	MATERIALS AND METHODS Dried and crushed aerial parts of A. italica were extracted with 75% ethanol to yield crude extract (AICE) and AICE was fractionated to obtain petroleum ether extract (AIPE), dichloromethane extract (AIDE), ethyl acetate extract (AIEE), n-butanol extract (AIBE) and residues (AIW).
34883218	17	41	theme	domain-containing	2746:2762	arg1	pyrin					2740:2744	pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3	2688:2764	pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3 (NLRP3)	2688:2772	In addition, the protein expression of pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3 (NLRP3) and IL-1β were decreased after the AIDE treatment.
34883218	17	41	theme	domain-containing	2746:2762	arg1	NLRP3					2767:2771	NLRP3	2767:2771	NLRP3	2767:2771	In addition, the protein expression of pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3 (NLRP3) and IL-1β were decreased after the AIDE treatment.
34883218	1	42	theme	NF-κB	121:125	arg1	pathways					137:144	NF-κB signaling pathways	121:144	NF-κB signaling pathways	121:144	in LPS-stimulated RAW264.7 cells mediated by the Nrf2/HO-1, MAPK and NF-κB signaling pathways.
34883218	3	43	theme	Uygur	308:312	arg1	medicines					314:322	traditional Uygur medicines	296:322	traditional Uygur medicines	296:322	(Boraginaceae) is an important medicinal plant for the treatment of meningitis and pneumonia in traditional Uygur medicines.
34883218	14	44	theme	IL-1β	2156:2160	arg1	over-expression					2121:2135	LPS-induced the over-expression	2105:2135	LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α	2105:2170	AIDE could inhibit LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α and down-regulate the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation.
34883218	10	45	theme	GSTM1	1655:1659	arg1	mRNA					1662:1665	M 1 (GSTM1) mRNA	1650:1665	glutathione S-transferase M 1 (GSTM1) mRNA	1624:1665	Real-time quantitative PCR was used to detect the expression of hemeoxygenase-1 (HO-1), Nrf2-mediated quinone oxidoreductase 1 (NQO-1), glutathione S-transferase A 1 (GSTA1) and glutathione S-transferase M 1 (GSTM1) mRNA.
34883218	5	46	theme	aerial	533:538	arg1	parts					540:544	aerial parts	533:544	aerial parts of A. italica	533:558	MATERIALS AND METHODS Dried and crushed aerial parts of A. italica were extracted with 75% ethanol to yield crude extract (AICE) and AICE was fractionated to obtain petroleum ether extract (AIPE), dichloromethane extract (AIDE), ethyl acetate extract (AIEE), n-butanol extract (AIBE) and residues (AIW).
34883218	17	47	theme	nod-like	2715:2722	arg1	pyrin					2740:2744	pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3	2688:2764	pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3 (NLRP3)	2688:2772	In addition, the protein expression of pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3 (NLRP3) and IL-1β were decreased after the AIDE treatment.
34883218	17	47	theme	nod-like	2715:2722	arg1	NLRP3					2767:2771	NLRP3	2767:2771	NLRP3	2767:2771	In addition, the protein expression of pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3 (NLRP3) and IL-1β were decreased after the AIDE treatment.
34883218	16	48	theme	AIDE	2557:2560	arg1	treatment					2544:2552	the treatment	2540:2552	the treatment of AIDE	2540:2560	After the treatment of AIDE, P65 nuclear translocation was inhibited and Nrf2 nuclear translocation was increased.
34883218	14	49	theme	PGE2	2150:2153	arg1	over-expression					2121:2135	LPS-induced the over-expression	2105:2135	LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α	2105:2170	AIDE could inhibit LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α and down-regulate the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation.
34883218	19	50	theme	Nrf2/HO-1	3094:3102	arg1	pathway					3104:3110	the Nrf2/HO-1 pathway	3090:3110	the Nrf2/HO-1 pathway	3090:3110	exerted its anti-inflammatory activity by inhibiting the mitogen-activated protein kinase (MAPK), nuclear transcription factors κB (NF-κB) and pyrolytic relevant proteins, down-regulating inflammatory factor levels, and activating the Nrf2/HO-1 pathway.
34883218	5	51	theme	ethyl	722:726	arg1	AIEE					745:748	AIEE	745:748	AIEE	745:748	MATERIALS AND METHODS Dried and crushed aerial parts of A. italica were extracted with 75% ethanol to yield crude extract (AICE) and AICE was fractionated to obtain petroleum ether extract (AIPE), dichloromethane extract (AIDE), ethyl acetate extract (AIEE), n-butanol extract (AIBE) and residues (AIW).
34883218	5	51	theme	ethyl	722:726	arg1	extract					736:742	ethyl acetate extract	722:742	ethyl acetate extract (AIEE)	722:749	MATERIALS AND METHODS Dried and crushed aerial parts of A. italica were extracted with 75% ethanol to yield crude extract (AICE) and AICE was fractionated to obtain petroleum ether extract (AIPE), dichloromethane extract (AIDE), ethyl acetate extract (AIEE), n-butanol extract (AIBE) and residues (AIW).
34883218	17	52	theme	receptor	2724:2731	arg1	pyrin					2740:2744	pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3	2688:2764	pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3 (NLRP3)	2688:2772	In addition, the protein expression of pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3 (NLRP3) and IL-1β were decreased after the AIDE treatment.
34883218	17	52	theme	receptor	2724:2731	arg1	NLRP3					2767:2771	NLRP3	2767:2771	NLRP3	2767:2771	In addition, the protein expression of pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3 (NLRP3) and IL-1β were decreased after the AIDE treatment.
34883218	9	53	theme	necrosis	1379:1386	arg1	factor					1388:1393	tumor necrosis factor α	1373:1395	tumor necrosis factor α (TNF-α)	1373:1403	Prostaglandin E2 (PGE2), interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α) production was examined by ELISA assays.
34883218	9	53	theme	necrosis	1379:1386	arg1	TNF-α					1398:1402	TNF-α	1398:1402	TNF-α	1398:1402	Prostaglandin E2 (PGE2), interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α) production was examined by ELISA assays.
34883218	20	54	theme	anti-inflammatory	3153:3169	arg1	ingredients					3171:3181	its major active anti-inflammatory ingredients	3136:3181	its major active anti-inflammatory ingredients	3136:3181	Triterpenoids might be its major active anti-inflammatory ingredients.
34883218	14	55	theme	IL-6	2144:2147	arg1	over-expression					2121:2135	LPS-induced the over-expression	2105:2135	LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α	2105:2170	AIDE could inhibit LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α and down-regulate the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation.
34883218	20	56	theme	major	3140:3144	arg1	ingredients					3171:3181	its major active anti-inflammatory ingredients	3136:3181	its major active anti-inflammatory ingredients	3136:3181	Triterpenoids might be its major active anti-inflammatory ingredients.
34883218	14	57	theme	NO	2140:2141	arg1	over-expression					2121:2135	LPS-induced the over-expression	2105:2135	LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α	2105:2170	AIDE could inhibit LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α and down-regulate the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation.
34883218	15	58	theme	protein	2445:2451	arg1	level					2464:2468	the protein expression level	2441:2468	the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1	2441:2531	It promoted the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1 and the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1.
34883218	11	59	theme	protein	1718:1724	arg1	expression					1726:1735	the protein expression	1714:1735	the protein expression	1714:1735	Western blot analysis was employed to examine the protein expression and enzymatic activities.
34883218	7	60	theme	LC-MS/MS	1086:1093	arg1	experiment					1095:1104	LC-MS/MS experiment	1086:1104	LC-MS/MS experiment	1086:1104	LC-MS/MS experiment was applied to analyze the chemical composition of AIDE.
34883218	5	61	theme	75	580:581	arg1	%					582:582	%	582:582	%	582:582	MATERIALS AND METHODS Dried and crushed aerial parts of A. italica were extracted with 75% ethanol to yield crude extract (AICE) and AICE was fractionated to obtain petroleum ether extract (AIPE), dichloromethane extract (AIDE), ethyl acetate extract (AIEE), n-butanol extract (AIBE) and residues (AIW).
34883218	18	62	dep	CONCLUSIONS	2825:2835	arg1	Retz					2853:2856	Retz	2853:2856	Retz	2853:2856	CONCLUSIONS Anchusa italica Retz.
34883218	14	63	theme	LPS-induced	2105:2115	arg1	over-expression					2121:2135	LPS-induced the over-expression	2105:2135	LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α	2105:2170	AIDE could inhibit LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α and down-regulate the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation.
34883218	0	64	theme	Anti-inflammatory	0:16	arg1	activity					18:25	Anti-inflammatory activity	0:25	Anti-inflammatory activity of Anchusa	0:36	Anti-inflammatory activity of Anchusa italica Retz.
34883218	6	65	theme	RAW264.7 cell	935:947	arg1	lines					949:953	RAW264.7 cell lines	935:953	RAW264.7 cell lines	935:953	By measuring the effects of AIPE, AIDE, AIEE, AIBE and AIW on cell viability and nitric oxide (NO) in Lipopolysaccharide (LPS) stimulated RAW264.7 cell lines, AIDE with the lowest cytotoxicity and NO contents was finally selected for further chemical and anti-inflammatory investigations.
34883218	8	66	theme	culture	1263:1269	arg1	supernatants					1271:1282	culture supernatants	1263:1282	culture supernatants	1263:1282	MTT and Griess methods were used to detect the cell viability and to quantify the nitrite levels in culture supernatants, respectively.
34883218	17	67	theme	pyrolytic	2688:2696	arg1	pyrin					2740:2744	pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3	2688:2764	pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3 (NLRP3)	2688:2772	In addition, the protein expression of pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3 (NLRP3) and IL-1β were decreased after the AIDE treatment.
34883218	17	67	theme	pyrolytic	2688:2696	arg1	NLRP3					2767:2771	NLRP3	2767:2771	NLRP3	2767:2771	In addition, the protein expression of pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3 (NLRP3) and IL-1β were decreased after the AIDE treatment.
34883218	13	68	theme	LC-MS/MS	2067:2074	arg1	analysis					2076:2083	LC-MS/MS analysis	2067:2083	LC-MS/MS analysis	2067:2083	Thirty-three compounds including twenty-five triterpenoids were identified by LC-MS/MS analysis.
34883218	14	69	theme	P65	2345:2347	arg1	phosphorylation					2350:2364	κB-P65 (P65) phosphorylation	2337:2364	κB-P65 (P65) phosphorylation	2337:2364	AIDE could inhibit LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α and down-regulate the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation.
34883218	9	70	theme	ELISA	1432:1436	arg1	assays					1438:1443	ELISA assays	1432:1443	ELISA assays	1432:1443	Prostaglandin E2 (PGE2), interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α) production was examined by ELISA assays.
34883218	19	71	theme	relevant	3012:3019	arg1	proteins					3021:3028	pyrolytic relevant proteins	3002:3028	pyrolytic relevant proteins	3002:3028	exerted its anti-inflammatory activity by inhibiting the mitogen-activated protein kinase (MAPK), nuclear transcription factors κB (NF-κB) and pyrolytic relevant proteins, down-regulating inflammatory factor levels, and activating the Nrf2/HO-1 pathway.
34883218	17	72	theme	protein	2707:2713	arg1	pyrin					2740:2744	pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3	2688:2764	pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3 (NLRP3)	2688:2772	In addition, the protein expression of pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3 (NLRP3) and IL-1β were decreased after the AIDE treatment.
34883218	17	72	theme	protein	2707:2713	arg1	NLRP3					2767:2771	NLRP3	2767:2771	NLRP3	2767:2771	In addition, the protein expression of pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3 (NLRP3) and IL-1β were decreased after the AIDE treatment.
34883218	15	73	theme	factor	2508:2513	arg1	level					2464:2468	the protein expression level	2441:2468	the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1	2441:2531	It promoted the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1 and the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1.
34883218	15	73	theme	factor	2508:2513	arg1	level					2399:2403	the mRNA expression level	2379:2403	the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1	2379:2435	It promoted the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1 and the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1.
34883218	6	74	with	AIDE	956:959	arg1	cytotoxicity					977:988	the lowest cytotoxicity	966:988	the lowest cytotoxicity	966:988	By measuring the effects of AIPE, AIDE, AIEE, AIBE and AIW on cell viability and nitric oxide (NO) in Lipopolysaccharide (LPS) stimulated RAW264.7 cell lines, AIDE with the lowest cytotoxicity and NO contents was finally selected for further chemical and anti-inflammatory investigations.
34883218	6	74	with	AIDE	956:959	arg1	contents					997:1004	NO contents	994:1004	NO contents	994:1004	By measuring the effects of AIPE, AIDE, AIEE, AIBE and AIW on cell viability and nitric oxide (NO) in Lipopolysaccharide (LPS) stimulated RAW264.7 cell lines, AIDE with the lowest cytotoxicity and NO contents was finally selected for further chemical and anti-inflammatory investigations.
34883218	15	75	theme	nuclear	2473:2479	arg1	factor					2508:2513	nuclear factor erythroid 2-related factor 2	2473:2515	nuclear factor erythroid 2-related factor 2 (Nrf2)	2473:2522	It promoted the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1 and the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1.
34883218	15	75	theme	nuclear	2473:2479	arg1	Nrf2					2518:2521	Nrf2	2518:2521	Nrf2	2518:2521	It promoted the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1 and the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1.
34883218	14	76	theme	c-Jun	2257:2261	arg1	JNK					2282:2284	JNK	2282:2284	JNK	2282:2284	AIDE could inhibit LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α and down-regulate the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation.
34883218	14	76	theme	c-Jun	2257:2261	arg1	kinase					2274:2279	c-Jun N-terminal kinase	2257:2279	c-Jun N-terminal kinase (JNK)	2257:2285	AIDE could inhibit LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α and down-regulate the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation.
34883218	11	77	theme	Western	1668:1674	arg1	analysis					1681:1688	Western blot analysis	1668:1688	Western blot analysis	1668:1688	Western blot analysis was employed to examine the protein expression and enzymatic activities.
34883218	15	78	theme	erythroid	2488:2496	arg1	factor					2508:2513	nuclear factor erythroid 2-related factor 2	2473:2515	nuclear factor erythroid 2-related factor 2 (Nrf2)	2473:2522	It promoted the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1 and the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1.
34883218	15	78	theme	erythroid	2488:2496	arg1	Nrf2					2518:2521	Nrf2	2518:2521	Nrf2	2518:2521	It promoted the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1 and the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1.
34883218	3	79	from	plant	241:245	arg1	medicines					314:322	traditional Uygur medicines	296:322	traditional Uygur medicines	296:322	(Boraginaceae) is an important medicinal plant for the treatment of meningitis and pneumonia in traditional Uygur medicines.
34883218	14	80	theme	transcription	2315:2327	arg1	factors					2329:2335	nuclear transcription factors	2307:2335	nuclear transcription factors	2307:2335	AIDE could inhibit LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α and down-regulate the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation.
34883218	14	81	theme	κB-P65	2337:2342	arg1	phosphorylation					2350:2364	κB-P65 (P65) phosphorylation	2337:2364	κB-P65 (P65) phosphorylation	2337:2364	AIDE could inhibit LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α and down-regulate the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation.
34883218	10	82	theme	Nrf2-mediated	1534:1546	arg1	oxidoreductase					1556:1569	Nrf2-mediated quinone oxidoreductase 1	1534:1571	Nrf2-mediated quinone oxidoreductase 1 (NQO-1)	1534:1579	Real-time quantitative PCR was used to detect the expression of hemeoxygenase-1 (HO-1), Nrf2-mediated quinone oxidoreductase 1 (NQO-1), glutathione S-transferase A 1 (GSTA1) and glutathione S-transferase M 1 (GSTM1) mRNA.
34883218	10	82	theme	Nrf2-mediated	1534:1546	arg1	NQO-1					1574:1578	NQO-1	1574:1578	NQO-1	1574:1578	Real-time quantitative PCR was used to detect the expression of hemeoxygenase-1 (HO-1), Nrf2-mediated quinone oxidoreductase 1 (NQO-1), glutathione S-transferase A 1 (GSTA1) and glutathione S-transferase M 1 (GSTM1) mRNA.
34883218	10	82	theme	Nrf2-mediated	1534:1546	arg1	hemeoxygenase-1					1510:1524	hemeoxygenase-1	1510:1524	hemeoxygenase-1 (HO-1)	1510:1531	Real-time quantitative PCR was used to detect the expression of hemeoxygenase-1 (HO-1), Nrf2-mediated quinone oxidoreductase 1 (NQO-1), glutathione S-transferase A 1 (GSTA1) and glutathione S-transferase M 1 (GSTM1) mRNA.
34883218	15	83	theme	expression	2388:2397	arg1	level					2399:2403	the mRNA expression level	2379:2403	the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1	2379:2435	It promoted the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1 and the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1.
34883218	16	84	theme	nuclear	2567:2573	arg1	translocation					2575:2587	P65 nuclear translocation	2563:2587	P65 nuclear translocation	2563:2587	After the treatment of AIDE, P65 nuclear translocation was inhibited and Nrf2 nuclear translocation was increased.
34883218	11	85	theme	enzymatic	1741:1749	arg1	activities					1751:1760	enzymatic activities	1741:1760	enzymatic activities	1741:1760	Western blot analysis was employed to examine the protein expression and enzymatic activities.
34883218	13	86	theme	Thirty-three	1989:2000	arg1	triterpenoids					2034:2046	twenty-five triterpenoids	2022:2046	twenty-five triterpenoids	2022:2046	Thirty-three compounds including twenty-five triterpenoids were identified by LC-MS/MS analysis.
34883218	13	86	theme	Thirty-three	1989:2000	arg1	compounds					2002:2010	Thirty-three compounds	1989:2010	Thirty-three compounds including twenty-five triterpenoids	1989:2046	Thirty-three compounds including twenty-five triterpenoids were identified by LC-MS/MS analysis.
34883218	14	87	theme	P38	2298:2300	arg1	levels					2194:2199	the levels	2190:2199	the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation	2190:2364	AIDE could inhibit LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α and down-regulate the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation.
34883218	7	88	theme	AIDE	1157:1160	arg1	composition					1142:1152	the chemical composition	1129:1152	the chemical composition of AIDE	1129:1160	LC-MS/MS experiment was applied to analyze the chemical composition of AIDE.
34883218	15	89	theme	expression	2453:2462	arg1	level					2464:2468	the protein expression level	2441:2468	the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1	2441:2531	It promoted the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1 and the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1.
34883218	14	90	theme	protein	2235:2241	arg1	ERK					2251:2253	ERK	2251:2253	ERK	2251:2253	AIDE could inhibit LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α and down-regulate the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation.
34883218	14	90	theme	protein	2235:2241	arg1	kinase					2243:2248	extracellular signal-regulated protein kinase	2204:2248	extracellular signal-regulated protein kinase (ERK)	2204:2254	AIDE could inhibit LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α and down-regulate the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation.
34883218	9	91	theme	Prostaglandin	1299:1311	arg1	E2					1313:1314	Prostaglandin E2	1299:1314	Prostaglandin E2 (PGE2)	1299:1321	Prostaglandin E2 (PGE2), interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α) production was examined by ELISA assays.
34883218	9	91	theme	Prostaglandin	1299:1311	arg1	PGE2					1317:1320	PGE2	1317:1320	PGE2	1317:1320	Prostaglandin E2 (PGE2), interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α) production was examined by ELISA assays.
34883218	12	92	theme	significant	1864:1874	arg1	effect					1887:1892	the most significant inhibitory effect	1855:1892	the most significant inhibitory effect on the production of NO (the inhibitory is 89%) induced by LPS	1855:1955	RESULTS In preliminary anti-inflammatory screening, AIDE showed the lowest cytotoxicity and the most significant inhibitory effect on the production of NO (the inhibitory is 89%) induced by LPS among the tested five extracts.
34883218	14	93	theme	P38-MAPK	2288:2295	arg1	P38					2298:2300	P38-MAPK (P38)	2288:2301	P38-MAPK (P38)	2288:2301	AIDE could inhibit LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α and down-regulate the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation.
34883218	14	94	theme	extracellular	2204:2216	arg1	ERK					2251:2253	ERK	2251:2253	ERK	2251:2253	AIDE could inhibit LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α and down-regulate the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation.
34883218	14	94	theme	extracellular	2204:2216	arg1	kinase					2243:2248	extracellular signal-regulated protein kinase	2204:2248	extracellular signal-regulated protein kinase (ERK)	2204:2254	AIDE could inhibit LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α and down-regulate the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation.
34883218	3	95	theme	medicinal	231:239	arg1	plant					241:245	an important medicinal plant	218:245	an important medicinal plant for the treatment of meningitis and pneumonia in traditional Uygur medicines	218:322	(Boraginaceae) is an important medicinal plant for the treatment of meningitis and pneumonia in traditional Uygur medicines.
34883218	4	96	theme	molecular	413:421	arg1	mechanisms					423:432	its molecular mechanisms	409:432	its molecular mechanisms	409:432	AIM OF THE STUDY To clarify the anti-inflammatory activity of A. italica, to reveal its molecular mechanisms, and to discover the anti-inflammatory active ingredients.
34883218	6	97	theme	anti-inflammatory	1052:1068	arg1	investigations					1070:1083	further chemical and anti-inflammatory investigations	1031:1083	further chemical and anti-inflammatory investigations	1031:1083	By measuring the effects of AIPE, AIDE, AIEE, AIBE and AIW on cell viability and nitric oxide (NO) in Lipopolysaccharide (LPS) stimulated RAW264.7 cell lines, AIDE with the lowest cytotoxicity and NO contents was finally selected for further chemical and anti-inflammatory investigations.
34883218	10	98	theme	glutathione	1582:1592	arg1	S-transferase					1594:1606	glutathione S-transferase A 1 (GSTA1)	1582:1618	glutathione S-transferase A 1 (GSTA1)	1582:1618	Real-time quantitative PCR was used to detect the expression of hemeoxygenase-1 (HO-1), Nrf2-mediated quinone oxidoreductase 1 (NQO-1), glutathione S-transferase A 1 (GSTA1) and glutathione S-transferase M 1 (GSTM1) mRNA.
34883218	10	98	theme	glutathione	1582:1592	arg1	hemeoxygenase-1					1510:1524	hemeoxygenase-1	1510:1524	hemeoxygenase-1 (HO-1)	1510:1531	Real-time quantitative PCR was used to detect the expression of hemeoxygenase-1 (HO-1), Nrf2-mediated quinone oxidoreductase 1 (NQO-1), glutathione S-transferase A 1 (GSTA1) and glutathione S-transferase M 1 (GSTM1) mRNA.
34883218	3	99	theme	meningitis	268:277	arg1	treatment					255:263	the treatment	251:263	the treatment of meningitis and pneumonia	251:291	(Boraginaceae) is an important medicinal plant for the treatment of meningitis and pneumonia in traditional Uygur medicines.
34883218	6	100	theme	chemical	1039:1046	arg1	investigations					1070:1083	further chemical and anti-inflammatory investigations	1031:1083	further chemical and anti-inflammatory investigations	1031:1083	By measuring the effects of AIPE, AIDE, AIEE, AIBE and AIW on cell viability and nitric oxide (NO) in Lipopolysaccharide (LPS) stimulated RAW264.7 cell lines, AIDE with the lowest cytotoxicity and NO contents was finally selected for further chemical and anti-inflammatory investigations.
34883218	10	101	theme	quantitative	1456:1467	arg1	PCR					1469:1471	Real-time quantitative PCR	1446:1471	Real-time quantitative PCR	1446:1471	Real-time quantitative PCR was used to detect the expression of hemeoxygenase-1 (HO-1), Nrf2-mediated quinone oxidoreductase 1 (NQO-1), glutathione S-transferase A 1 (GSTA1) and glutathione S-transferase M 1 (GSTM1) mRNA.
34883218	9	102	theme	interleukin-6	1324:1336	arg1	production					1405:1414	Prostaglandin E2 (PGE2), interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α) production	1299:1414	Prostaglandin E2 (PGE2), interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α) production	1299:1414	Prostaglandin E2 (PGE2), interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α) production was examined by ELISA assays.
34883218	3	103	dep	plant	241:245	arg1	Boraginaceae					201:212	Boraginaceae	201:212	Boraginaceae	201:212	(Boraginaceae) is an important medicinal plant for the treatment of meningitis and pneumonia in traditional Uygur medicines.
34883218	14	104	theme	TNF-α	2166:2170	arg1	over-expression					2121:2135	LPS-induced the over-expression	2105:2135	LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α	2105:2170	AIDE could inhibit LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α and down-regulate the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation.
34883218	17	105	theme	AIDE	2809:2812	arg1	treatment					2814:2822	the AIDE treatment	2805:2822	the AIDE treatment	2805:2822	In addition, the protein expression of pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3 (NLRP3) and IL-1β were decreased after the AIDE treatment.
34883218	3	106	theme	traditional	296:306	arg1	medicines					314:322	traditional Uygur medicines	296:322	traditional Uygur medicines	296:322	(Boraginaceae) is an important medicinal plant for the treatment of meningitis and pneumonia in traditional Uygur medicines.
34883218	8	107	theme	cell	1210:1213	arg1	viability					1215:1223	the cell viability	1206:1223	the cell viability	1206:1223	MTT and Griess methods were used to detect the cell viability and to quantify the nitrite levels in culture supernatants, respectively.
34883218	10	108	theme	quinone	1548:1554	arg1	oxidoreductase					1556:1569	Nrf2-mediated quinone oxidoreductase 1	1534:1571	Nrf2-mediated quinone oxidoreductase 1 (NQO-1)	1534:1579	Real-time quantitative PCR was used to detect the expression of hemeoxygenase-1 (HO-1), Nrf2-mediated quinone oxidoreductase 1 (NQO-1), glutathione S-transferase A 1 (GSTA1) and glutathione S-transferase M 1 (GSTM1) mRNA.
34883218	10	108	theme	quinone	1548:1554	arg1	NQO-1					1574:1578	NQO-1	1574:1578	NQO-1	1574:1578	Real-time quantitative PCR was used to detect the expression of hemeoxygenase-1 (HO-1), Nrf2-mediated quinone oxidoreductase 1 (NQO-1), glutathione S-transferase A 1 (GSTA1) and glutathione S-transferase M 1 (GSTM1) mRNA.
34883218	10	108	theme	quinone	1548:1554	arg1	hemeoxygenase-1					1510:1524	hemeoxygenase-1	1510:1524	hemeoxygenase-1 (HO-1)	1510:1531	Real-time quantitative PCR was used to detect the expression of hemeoxygenase-1 (HO-1), Nrf2-mediated quinone oxidoreductase 1 (NQO-1), glutathione S-transferase A 1 (GSTA1) and glutathione S-transferase M 1 (GSTM1) mRNA.
34883218	10	109	theme	M	1650:1650	arg1	mRNA					1662:1665	M 1 (GSTM1) mRNA	1650:1665	glutathione S-transferase M 1 (GSTM1) mRNA	1624:1665	Real-time quantitative PCR was used to detect the expression of hemeoxygenase-1 (HO-1), Nrf2-mediated quinone oxidoreductase 1 (NQO-1), glutathione S-transferase A 1 (GSTA1) and glutathione S-transferase M 1 (GSTM1) mRNA.
34883218	3	110	theme	pneumonia	283:291	arg1	treatment					255:263	the treatment	251:263	the treatment of meningitis and pneumonia	251:291	(Boraginaceae) is an important medicinal plant for the treatment of meningitis and pneumonia in traditional Uygur medicines.
34883218	4	111	theme	active	473:478	arg1	ingredients					480:490	the anti-inflammatory active ingredients	451:490	the anti-inflammatory active ingredients	451:490	AIM OF THE STUDY To clarify the anti-inflammatory activity of A. italica, to reveal its molecular mechanisms, and to discover the anti-inflammatory active ingredients.
34883218	14	112	dep	kinase	2243:2248	arg1	phosphorylation					2350:2364	κB-P65 (P65) phosphorylation	2337:2364	κB-P65 (P65) phosphorylation	2337:2364	AIDE could inhibit LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α and down-regulate the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation.
34883218	10	113	dep	S-transferase	1636:1648	arg1	mRNA					1662:1665	M 1 (GSTM1) mRNA	1650:1665	glutathione S-transferase M 1 (GSTM1) mRNA	1624:1665	Real-time quantitative PCR was used to detect the expression of hemeoxygenase-1 (HO-1), Nrf2-mediated quinone oxidoreductase 1 (NQO-1), glutathione S-transferase A 1 (GSTA1) and glutathione S-transferase M 1 (GSTM1) mRNA.
34883218	5	114	theme	petroleum	658:666	arg1	extract					674:680	petroleum ether extract	658:680	petroleum ether extract (AIPE)	658:687	MATERIALS AND METHODS Dried and crushed aerial parts of A. italica were extracted with 75% ethanol to yield crude extract (AICE) and AICE was fractionated to obtain petroleum ether extract (AIPE), dichloromethane extract (AIDE), ethyl acetate extract (AIEE), n-butanol extract (AIBE) and residues (AIW).
34883218	5	114	theme	petroleum	658:666	arg1	AIPE					683:686	AIPE	683:686	AIPE	683:686	MATERIALS AND METHODS Dried and crushed aerial parts of A. italica were extracted with 75% ethanol to yield crude extract (AICE) and AICE was fractionated to obtain petroleum ether extract (AIPE), dichloromethane extract (AIDE), ethyl acetate extract (AIEE), n-butanol extract (AIBE) and residues (AIW).
34883218	10	115	theme	glutathione	1624:1634	arg1	S-transferase					1636:1648	glutathione S-transferase M 1 (GSTM1) mRNA	1624:1665	glutathione S-transferase M 1 (GSTM1) mRNA	1624:1665	Real-time quantitative PCR was used to detect the expression of hemeoxygenase-1 (HO-1), Nrf2-mediated quinone oxidoreductase 1 (NQO-1), glutathione S-transferase A 1 (GSTA1) and glutathione S-transferase M 1 (GSTM1) mRNA.
34883218	10	115	theme	glutathione	1624:1634	arg1	hemeoxygenase-1					1510:1524	hemeoxygenase-1	1510:1524	hemeoxygenase-1 (HO-1)	1510:1531	Real-time quantitative PCR was used to detect the expression of hemeoxygenase-1 (HO-1), Nrf2-mediated quinone oxidoreductase 1 (NQO-1), glutathione S-transferase A 1 (GSTA1) and glutathione S-transferase M 1 (GSTM1) mRNA.
34883218	12	116	theme	preliminary	1774:1784	arg1	AIDE					1815:1818	AIDE	1815:1818	AIDE	1815:1818	RESULTS In preliminary anti-inflammatory screening, AIDE showed the lowest cytotoxicity and the most significant inhibitory effect on the production of NO (the inhibitory is 89%) induced by LPS among the tested five extracts.
34883218	12	116	theme	preliminary	1774:1784	arg1	screening					1804:1812	preliminary anti-inflammatory screening	1774:1812	preliminary anti-inflammatory screening	1774:1812	RESULTS In preliminary anti-inflammatory screening, AIDE showed the lowest cytotoxicity and the most significant inhibitory effect on the production of NO (the inhibitory is 89%) induced by LPS among the tested five extracts.
34883218	19	117	theme	mitogen-activated	2916:2932	arg1	MAPK					2950:2953	MAPK	2950:2953	MAPK	2950:2953	exerted its anti-inflammatory activity by inhibiting the mitogen-activated protein kinase (MAPK), nuclear transcription factors κB (NF-κB) and pyrolytic relevant proteins, down-regulating inflammatory factor levels, and activating the Nrf2/HO-1 pathway.
34883218	19	117	theme	mitogen-activated	2916:2932	arg1	kinase					2942:2947	the mitogen-activated protein kinase	2912:2947	the mitogen-activated protein kinase (MAPK)	2912:2954	exerted its anti-inflammatory activity by inhibiting the mitogen-activated protein kinase (MAPK), nuclear transcription factors κB (NF-κB) and pyrolytic relevant proteins, down-regulating inflammatory factor levels, and activating the Nrf2/HO-1 pathway.
34883218	1	118	theme	signaling	127:135	arg1	pathways					137:144	NF-κB signaling pathways	121:144	NF-κB signaling pathways	121:144	in LPS-stimulated RAW264.7 cells mediated by the Nrf2/HO-1, MAPK and NF-κB signaling pathways.
34883218	5	119	dep	MATERIALS	493:501	arg1	Dried					515:519	Dried	515:519	Dried	515:519	MATERIALS AND METHODS Dried and crushed aerial parts of A. italica were extracted with 75% ethanol to yield crude extract (AICE) and AICE was fractionated to obtain petroleum ether extract (AIPE), dichloromethane extract (AIDE), ethyl acetate extract (AIEE), n-butanol extract (AIBE) and residues (AIW).
34883218	5	119	dep	MATERIALS	493:501	arg1	crushed					525:531	crushed	525:531	crushed aerial parts of A. italica	525:558	MATERIALS AND METHODS Dried and crushed aerial parts of A. italica were extracted with 75% ethanol to yield crude extract (AICE) and AICE was fractionated to obtain petroleum ether extract (AIPE), dichloromethane extract (AIDE), ethyl acetate extract (AIEE), n-butanol extract (AIBE) and residues (AIW).
34883218	19	120	theme	transcription	2965:2977	arg1	factors					2979:2985	nuclear transcription factors κB (NF-κB)	2957:2996	nuclear transcription factors κB (NF-κB)	2957:2996	exerted its anti-inflammatory activity by inhibiting the mitogen-activated protein kinase (MAPK), nuclear transcription factors κB (NF-κB) and pyrolytic relevant proteins, down-regulating inflammatory factor levels, and activating the Nrf2/HO-1 pathway.
34883218	9	121	theme	tumor	1373:1377	arg1	factor					1388:1393	tumor necrosis factor α	1373:1395	tumor necrosis factor α (TNF-α)	1373:1403	Prostaglandin E2 (PGE2), interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α) production was examined by ELISA assays.
34883218	9	121	theme	tumor	1373:1377	arg1	TNF-α					1398:1402	TNF-α	1398:1402	TNF-α	1398:1402	Prostaglandin E2 (PGE2), interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α) production was examined by ELISA assays.
34883218	1	122	dep	MAPK	112:115	arg1	RAW264.7 cells					70:83	LPS-stimulated RAW264.7 cells	55:83	LPS-stimulated RAW264.7 cells mediated by the Nrf2/HO-1	55:109	in LPS-stimulated RAW264.7 cells mediated by the Nrf2/HO-1, MAPK and NF-κB signaling pathways.
34883218	5	123	theme	acetate	728:734	arg1	AIEE					745:748	AIEE	745:748	AIEE	745:748	MATERIALS AND METHODS Dried and crushed aerial parts of A. italica were extracted with 75% ethanol to yield crude extract (AICE) and AICE was fractionated to obtain petroleum ether extract (AIPE), dichloromethane extract (AIDE), ethyl acetate extract (AIEE), n-butanol extract (AIBE) and residues (AIW).
34883218	5	123	theme	acetate	728:734	arg1	extract					736:742	ethyl acetate extract	722:742	ethyl acetate extract (AIEE)	722:749	MATERIALS AND METHODS Dried and crushed aerial parts of A. italica were extracted with 75% ethanol to yield crude extract (AICE) and AICE was fractionated to obtain petroleum ether extract (AIPE), dichloromethane extract (AIDE), ethyl acetate extract (AIEE), n-butanol extract (AIBE) and residues (AIW).
34883218	12	124	from	effect	1887:1892	arg1	production					1901:1910	the production	1897:1910	the production of NO (the inhibitory is 89%) induced by LPS	1897:1955	RESULTS In preliminary anti-inflammatory screening, AIDE showed the lowest cytotoxicity and the most significant inhibitory effect on the production of NO (the inhibitory is 89%) induced by LPS among the tested five extracts.
34883218	9	125	theme	factor	1388:1393	arg1	production					1405:1414	Prostaglandin E2 (PGE2), interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α) production	1299:1414	Prostaglandin E2 (PGE2), interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α) production	1299:1414	Prostaglandin E2 (PGE2), interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α) production was examined by ELISA assays.
34883218	20	126	theme	active	3146:3151	arg1	ingredients					3171:3181	its major active anti-inflammatory ingredients	3136:3181	its major active anti-inflammatory ingredients	3136:3181	Triterpenoids might be its major active anti-inflammatory ingredients.
34883218	10	127	dep	S-transferase	1594:1606	arg1	A					1608:1608	A 1	1608:1610	glutathione S-transferase A 1 (GSTA1)	1582:1618	Real-time quantitative PCR was used to detect the expression of hemeoxygenase-1 (HO-1), Nrf2-mediated quinone oxidoreductase 1 (NQO-1), glutathione S-transferase A 1 (GSTA1) and glutathione S-transferase M 1 (GSTM1) mRNA.
34883218	10	127	dep	S-transferase	1594:1606	arg1	GSTA1					1613:1617	GSTA1	1613:1617	GSTA1	1613:1617	Real-time quantitative PCR was used to detect the expression of hemeoxygenase-1 (HO-1), Nrf2-mediated quinone oxidoreductase 1 (NQO-1), glutathione S-transferase A 1 (GSTA1) and glutathione S-transferase M 1 (GSTM1) mRNA.
34883218	19	128	theme	nuclear	2957:2963	arg1	factors					2979:2985	nuclear transcription factors κB (NF-κB)	2957:2996	nuclear transcription factors κB (NF-κB)	2957:2996	exerted its anti-inflammatory activity by inhibiting the mitogen-activated protein kinase (MAPK), nuclear transcription factors κB (NF-κB) and pyrolytic relevant proteins, down-regulating inflammatory factor levels, and activating the Nrf2/HO-1 pathway.
34883218	5	129	theme	crude	601:605	arg1	AICE					616:619	AICE	616:619	AICE	616:619	MATERIALS AND METHODS Dried and crushed aerial parts of A. italica were extracted with 75% ethanol to yield crude extract (AICE) and AICE was fractionated to obtain petroleum ether extract (AIPE), dichloromethane extract (AIDE), ethyl acetate extract (AIEE), n-butanol extract (AIBE) and residues (AIW).
34883218	5	129	theme	crude	601:605	arg1	extract					607:613	crude extract	601:613	crude extract (AICE)	601:620	MATERIALS AND METHODS Dried and crushed aerial parts of A. italica were extracted with 75% ethanol to yield crude extract (AICE) and AICE was fractionated to obtain petroleum ether extract (AIPE), dichloromethane extract (AIDE), ethyl acetate extract (AIEE), n-butanol extract (AIBE) and residues (AIW).
34883218	1	130	theme	LPS-stimulated	55:68	arg1	RAW264.7 cells					70:83	LPS-stimulated RAW264.7 cells	55:83	LPS-stimulated RAW264.7 cells mediated by the Nrf2/HO-1	55:109	in LPS-stimulated RAW264.7 cells mediated by the Nrf2/HO-1, MAPK and NF-κB signaling pathways.
34883218	6	131	theme	nitric	878:883	arg1	oxide					885:889	nitric oxide	878:889	nitric oxide (NO)	878:894	By measuring the effects of AIPE, AIDE, AIEE, AIBE and AIW on cell viability and nitric oxide (NO) in Lipopolysaccharide (LPS) stimulated RAW264.7 cell lines, AIDE with the lowest cytotoxicity and NO contents was finally selected for further chemical and anti-inflammatory investigations.
34883218	6	131	theme	nitric	878:883	arg1	NO					892:893	NO	892:893	NO	892:893	By measuring the effects of AIPE, AIDE, AIEE, AIBE and AIW on cell viability and nitric oxide (NO) in Lipopolysaccharide (LPS) stimulated RAW264.7 cell lines, AIDE with the lowest cytotoxicity and NO contents was finally selected for further chemical and anti-inflammatory investigations.
34883218	15	132	theme	GSTM1	2431:2435	arg1	level					2464:2468	the protein expression level	2441:2468	the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1	2441:2531	It promoted the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1 and the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1.
34883218	15	132	theme	GSTM1	2431:2435	arg1	level					2399:2403	the mRNA expression level	2379:2403	the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1	2379:2435	It promoted the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1 and the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1.
34883218	17	133	theme	protein	2666:2672	arg1	expression					2674:2683	the protein expression	2662:2683	the protein expression of pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3 (NLRP3) and IL-1β	2662:2782	In addition, the protein expression of pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3 (NLRP3) and IL-1β were decreased after the AIDE treatment.
34883218	5	134	theme	n-butanol	752:760	arg1	extract					762:768	n-butanol extract	752:768	n-butanol extract (AIBE)	752:775	MATERIALS AND METHODS Dried and crushed aerial parts of A. italica were extracted with 75% ethanol to yield crude extract (AICE) and AICE was fractionated to obtain petroleum ether extract (AIPE), dichloromethane extract (AIDE), ethyl acetate extract (AIEE), n-butanol extract (AIBE) and residues (AIW).
34883218	5	134	theme	n-butanol	752:760	arg1	AIBE					771:774	AIBE	771:774	AIBE	771:774	MATERIALS AND METHODS Dried and crushed aerial parts of A. italica were extracted with 75% ethanol to yield crude extract (AICE) and AICE was fractionated to obtain petroleum ether extract (AIPE), dichloromethane extract (AIDE), ethyl acetate extract (AIEE), n-butanol extract (AIBE) and residues (AIW).
34883218	19	135	theme	inflammatory	3047:3058	arg1	levels					3067:3072	inflammatory factor levels	3047:3072	inflammatory factor levels	3047:3072	exerted its anti-inflammatory activity by inhibiting the mitogen-activated protein kinase (MAPK), nuclear transcription factors κB (NF-κB) and pyrolytic relevant proteins, down-regulating inflammatory factor levels, and activating the Nrf2/HO-1 pathway.
34883218	5	136	theme	%	582:582	arg1	ethanol					584:590	75% ethanol	580:590	75% ethanol	580:590	MATERIALS AND METHODS Dried and crushed aerial parts of A. italica were extracted with 75% ethanol to yield crude extract (AICE) and AICE was fractionated to obtain petroleum ether extract (AIPE), dichloromethane extract (AIDE), ethyl acetate extract (AIEE), n-butanol extract (AIBE) and residues (AIW).
34883218	19	137	dep	factors	2979:2985	arg1	NF-κB					2991:2995	NF-κB	2991:2995	NF-κB	2991:2995	exerted its anti-inflammatory activity by inhibiting the mitogen-activated protein kinase (MAPK), nuclear transcription factors κB (NF-κB) and pyrolytic relevant proteins, down-regulating inflammatory factor levels, and activating the Nrf2/HO-1 pathway.
34883218	19	137	dep	factors	2979:2985	arg1	κB					2987:2988	κB	2987:2988	nuclear transcription factors κB (NF-κB)	2957:2996	exerted its anti-inflammatory activity by inhibiting the mitogen-activated protein kinase (MAPK), nuclear transcription factors κB (NF-κB) and pyrolytic relevant proteins, down-regulating inflammatory factor levels, and activating the Nrf2/HO-1 pathway.
34883218	8	138	theme	nitrite	1245:1251	arg1	levels					1253:1258	the nitrite levels	1241:1258	the nitrite levels in culture supernatants	1241:1282	MTT and Griess methods were used to detect the cell viability and to quantify the nitrite levels in culture supernatants, respectively.
34883218	0	139	theme	Anchusa	30:36	arg1	activity					18:25	Anti-inflammatory activity	0:25	Anti-inflammatory activity of Anchusa	0:36	Anti-inflammatory activity of Anchusa italica Retz.
34883218	8	140	used	used	1191:1194	arg2	methods					1178:1184	MTT and Griess methods	1163:1184	MTT and Griess methods	1163:1184	MTT and Griess methods were used to detect the cell viability and to quantify the nitrite levels in culture supernatants, respectively.
34883218	12	141	dep	cytotoxicity	1838:1849	arg1	the					1827:1829	the	1827:1829	the	1827:1829	RESULTS In preliminary anti-inflammatory screening, AIDE showed the lowest cytotoxicity and the most significant inhibitory effect on the production of NO (the inhibitory is 89%) induced by LPS among the tested five extracts.
34883218	19	142	theme	pyrolytic	3002:3010	arg1	proteins					3021:3028	pyrolytic relevant proteins	3002:3028	pyrolytic relevant proteins	3002:3028	exerted its anti-inflammatory activity by inhibiting the mitogen-activated protein kinase (MAPK), nuclear transcription factors κB (NF-κB) and pyrolytic relevant proteins, down-regulating inflammatory factor levels, and activating the Nrf2/HO-1 pathway.
34883218	17	143	theme	relevant	2698:2705	arg1	pyrin					2740:2744	pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3	2688:2764	pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3 (NLRP3)	2688:2772	In addition, the protein expression of pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3 (NLRP3) and IL-1β were decreased after the AIDE treatment.
34883218	17	143	theme	relevant	2698:2705	arg1	NLRP3					2767:2771	NLRP3	2767:2771	NLRP3	2767:2771	In addition, the protein expression of pyrolytic relevant protein nod-like receptor family pyrin domain-containing 3 (NLRP3) and IL-1β were decreased after the AIDE treatment.
34883218	15	144	theme	2-related	2498:2506	arg1	factor					2508:2513	nuclear factor erythroid 2-related factor 2	2473:2515	nuclear factor erythroid 2-related factor 2 (Nrf2)	2473:2522	It promoted the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1 and the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1.
34883218	15	144	theme	2-related	2498:2506	arg1	Nrf2					2518:2521	Nrf2	2518:2521	Nrf2	2518:2521	It promoted the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1 and the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1.
34883218	14	145	theme	N-terminal	2263:2272	arg1	JNK					2282:2284	JNK	2282:2284	JNK	2282:2284	AIDE could inhibit LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α and down-regulate the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation.
34883218	14	145	theme	N-terminal	2263:2272	arg1	kinase					2274:2279	c-Jun N-terminal kinase	2257:2279	c-Jun N-terminal kinase (JNK)	2257:2285	AIDE could inhibit LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α and down-regulate the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation.
34883218	10	146	theme	hemeoxygenase-1	1510:1524	arg1	expression					1496:1505	the expression	1492:1505	the expression of hemeoxygenase-1 (HO-1), Nrf2-mediated quinone oxidoreductase 1 (NQO-1), glutathione S-transferase A 1 (GSTA1) and glutathione S-transferase M 1 (GSTM1) mRNA	1492:1665	Real-time quantitative PCR was used to detect the expression of hemeoxygenase-1 (HO-1), Nrf2-mediated quinone oxidoreductase 1 (NQO-1), glutathione S-transferase A 1 (GSTA1) and glutathione S-transferase M 1 (GSTM1) mRNA.
34883218	15	147	theme	factor	2481:2486	arg1	factor					2508:2513	nuclear factor erythroid 2-related factor 2	2473:2515	nuclear factor erythroid 2-related factor 2 (Nrf2)	2473:2522	It promoted the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1 and the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1.
34883218	15	147	theme	factor	2481:2486	arg1	Nrf2					2518:2521	Nrf2	2518:2521	Nrf2	2518:2521	It promoted the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1 and the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1.
34883218	13	148	theme	twenty-five	2022:2032	arg1	triterpenoids					2034:2046	twenty-five triterpenoids	2022:2046	twenty-five triterpenoids	2022:2046	Thirty-three compounds including twenty-five triterpenoids were identified by LC-MS/MS analysis.
34883218	14	149	theme	factors	2329:2335	arg1	levels					2194:2199	the levels	2190:2199	the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation	2190:2364	AIDE could inhibit LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α and down-regulate the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation.
34883218	11	150	theme	blot	1676:1679	arg1	analysis					1681:1688	Western blot analysis	1668:1688	Western blot analysis	1668:1688	Western blot analysis was employed to examine the protein expression and enzymatic activities.
34883218	15	151	theme	mRNA	2383:2386	arg1	level					2399:2403	the mRNA expression level	2379:2403	the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1	2379:2435	It promoted the mRNA expression level of HO-1, NQO-1, GSTA1 and GSTM1 and the protein expression level of nuclear factor erythroid 2-related factor 2 (Nrf2) and HO-1.
34883218	14	152	theme	nuclear	2307:2313	arg1	factors					2329:2335	nuclear transcription factors	2307:2335	nuclear transcription factors	2307:2335	AIDE could inhibit LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α and down-regulate the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation.
34883218	4	153	theme	anti-inflammatory	357:373	arg1	activity					375:382	the anti-inflammatory activity	353:382	the anti-inflammatory activity of A. italica	353:396	AIM OF THE STUDY To clarify the anti-inflammatory activity of A. italica, to reveal its molecular mechanisms, and to discover the anti-inflammatory active ingredients.
34883218	6	154	theme	NO	994:995	arg1	contents					997:1004	NO contents	994:1004	NO contents	994:1004	By measuring the effects of AIPE, AIDE, AIEE, AIBE and AIW on cell viability and nitric oxide (NO) in Lipopolysaccharide (LPS) stimulated RAW264.7 cell lines, AIDE with the lowest cytotoxicity and NO contents was finally selected for further chemical and anti-inflammatory investigations.
34883218	6	155	theme	lowest	970:975	arg1	cytotoxicity					977:988	the lowest cytotoxicity	966:988	the lowest cytotoxicity	966:988	By measuring the effects of AIPE, AIDE, AIEE, AIBE and AIW on cell viability and nitric oxide (NO) in Lipopolysaccharide (LPS) stimulated RAW264.7 cell lines, AIDE with the lowest cytotoxicity and NO contents was finally selected for further chemical and anti-inflammatory investigations.
34883218	12	156	from	RESULTS	1763:1769	arg1	AIDE					1815:1818	AIDE	1815:1818	AIDE	1815:1818	RESULTS In preliminary anti-inflammatory screening, AIDE showed the lowest cytotoxicity and the most significant inhibitory effect on the production of NO (the inhibitory is 89%) induced by LPS among the tested five extracts.
34883218	12	156	from	RESULTS	1763:1769	arg1	screening					1804:1812	preliminary anti-inflammatory screening	1774:1812	preliminary anti-inflammatory screening	1774:1812	RESULTS In preliminary anti-inflammatory screening, AIDE showed the lowest cytotoxicity and the most significant inhibitory effect on the production of NO (the inhibitory is 89%) induced by LPS among the tested five extracts.
34883218	12	157	theme	tested	1967:1972	arg1	extracts					1979:1986	five extracts	1974:1986	the tested five extracts	1963:1986	RESULTS In preliminary anti-inflammatory screening, AIDE showed the lowest cytotoxicity and the most significant inhibitory effect on the production of NO (the inhibitory is 89%) induced by LPS among the tested five extracts.
34883218	14	158	theme	kinase	2243:2248	arg1	levels					2194:2199	the levels	2190:2199	the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation	2190:2364	AIDE could inhibit LPS-induced the over-expression of NO, IL-6, PGE2, IL-1β and TNF-α and down-regulate the levels of extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38-MAPK (P38) and nuclear transcription factors κB-P65 (P65) phosphorylation.
34013598	8	0	theme	reduced	1082:1088	arg1	cells					1094:1098	reduced B10 cells	1082:1098	reduced B10 cells	1082:1098	Adoptive transfer of B10 cells significantly attenuated collagen deposition whereas CD19-/- mice with reduced B10 cells exacerbated fibrosis following cardiac injury.
34013598	6	1	theme	M1/M2	753:757	arg1	Mc					759:760	M1/M2 Mc	753:760	M1/M2 Mc	753:760	hyaluronan-induced conversion of M1/M2 Mc was dependent on the size of hyaluronan.
34013598	4	2	theme	hyaluronan	566:575	arg1	secretion					577:585	hyaluronan secretion	566:585	hyaluronan secretion	566:585	We found that the frequency of B10 cells was significantly increased, and they participated in the regression of fibrosis via IL-10, particularly by accelerating hyaluronan secretion and inhibiting collagen deposition.
34013598	0	3	theme	IL-10	78:82	arg1	production					84:93	IL-10 production	78:93	IL-10 production	78:93	B10 cells decrease fibrosis progression following cardiac injury partially by IL-10 production and regulating hyaluronan secretion.
34013598	9	4	theme	promising	1356:1364	arg1	cells					1335:1339	B10 cells	1331:1339	B10 cells	1331:1339	Our results provide new evidence suggesting that B10 cells exert antifibrotic effects by regulating the extracellular matrix composition during cardiac injury, and also highlight that B10 cells may serve as a promising therapeutic candidate for managing cardiac fibrosis-associated disorders.
34013598	9	4	theme	promising	1356:1364	arg1	candidate					1378:1386	a promising therapeutic candidate	1354:1386	a promising therapeutic candidate for managing cardiac fibrosis-associated disorders	1354:1437	Our results provide new evidence suggesting that B10 cells exert antifibrotic effects by regulating the extracellular matrix composition during cardiac injury, and also highlight that B10 cells may serve as a promising therapeutic candidate for managing cardiac fibrosis-associated disorders.
34013598	1	5	theme	inflammatory	165:176	arg1	disorders					178:186	inflammatory disorders	165:186	inflammatory disorders	165:186	B10 cells play negative roles in inflammatory disorders by producing IL-10.
34013598	7	6	theme	high	888:891	arg1	weight					903:908	high molecular weight	888:908	high molecular weight	888:908	Low molecular weight hyaluronan promoted the conversion to M1 Mϕ, whereas medium and high molecular weight hyaluronan accelerated Mϕ transdifferentiation into the M2 phenotype.
34013598	3	7	theme	frequency	348:356	arg1	changes					328:334	the dynamic changes	316:334	the dynamic changes of B10 cell frequency	316:356	Therefore, this study was conducted to examine the dynamic changes of B10 cell frequency and their potential role in cardiac fibrosis.
34013598	3	7	theme	frequency	348:356	arg1	role					378:381	their potential role	362:381	their potential role in cardiac fibrosis	362:401	Therefore, this study was conducted to examine the dynamic changes of B10 cell frequency and their potential role in cardiac fibrosis.
34013598	9	8	theme	therapeutic	1366:1376	arg1	cells					1335:1339	B10 cells	1331:1339	B10 cells	1331:1339	Our results provide new evidence suggesting that B10 cells exert antifibrotic effects by regulating the extracellular matrix composition during cardiac injury, and also highlight that B10 cells may serve as a promising therapeutic candidate for managing cardiac fibrosis-associated disorders.
34013598	9	8	theme	therapeutic	1366:1376	arg1	candidate					1378:1386	a promising therapeutic candidate	1354:1386	a promising therapeutic candidate for managing cardiac fibrosis-associated disorders	1354:1437	Our results provide new evidence suggesting that B10 cells exert antifibrotic effects by regulating the extracellular matrix composition during cardiac injury, and also highlight that B10 cells may serve as a promising therapeutic candidate for managing cardiac fibrosis-associated disorders.
34013598	8	9	theme	cells	1005:1009	arg1	transfer					989:996	Adoptive transfer	980:996	Adoptive transfer of B10 cells	980:1009	Adoptive transfer of B10 cells significantly attenuated collagen deposition whereas CD19-/- mice with reduced B10 cells exacerbated fibrosis following cardiac injury.
34013598	7	10	theme	molecular	893:901	arg1	weight					903:908	high molecular weight	888:908	high molecular weight	888:908	Low molecular weight hyaluronan promoted the conversion to M1 Mϕ, whereas medium and high molecular weight hyaluronan accelerated Mϕ transdifferentiation into the M2 phenotype.
34013598	8	11	theme	following	1121:1129	arg1	injury					1139:1144	fibrosis following cardiac injury	1112:1144	fibrosis following cardiac injury	1112:1144	Adoptive transfer of B10 cells significantly attenuated collagen deposition whereas CD19-/- mice with reduced B10 cells exacerbated fibrosis following cardiac injury.
34013598	8	12	theme	B10	1090:1092	arg1	cells					1094:1098	reduced B10 cells	1082:1098	reduced B10 cells	1082:1098	Adoptive transfer of B10 cells significantly attenuated collagen deposition whereas CD19-/- mice with reduced B10 cells exacerbated fibrosis following cardiac injury.
34013598	8	13	theme	collagen	1036:1043	arg1	deposition					1045:1054	collagen deposition	1036:1054	collagen deposition	1036:1054	Adoptive transfer of B10 cells significantly attenuated collagen deposition whereas CD19-/- mice with reduced B10 cells exacerbated fibrosis following cardiac injury.
34013598	3	14	theme	B10	339:341	arg1	frequency					348:356	B10 cell frequency	339:356	B10 cell frequency	339:356	Therefore, this study was conducted to examine the dynamic changes of B10 cell frequency and their potential role in cardiac fibrosis.
34013598	8	15	theme	fibrosis	1112:1119	arg1	injury					1139:1144	fibrosis following cardiac injury	1112:1144	fibrosis following cardiac injury	1112:1144	Adoptive transfer of B10 cells significantly attenuated collagen deposition whereas CD19-/- mice with reduced B10 cells exacerbated fibrosis following cardiac injury.
34013598	2	16	from	effects	223:229	arg1	fibrosis					234:241	fibrosis	234:241	fibrosis	234:241	However, their effects on fibrosis have not been elucidated.
34013598	0	17	theme	hyaluronan	110:119	arg1	secretion					121:129	hyaluronan secretion	110:129	hyaluronan secretion	110:129	B10 cells decrease fibrosis progression following cardiac injury partially by IL-10 production and regulating hyaluronan secretion.
34013598	3	18	from	role	378:381	arg1	fibrosis					394:401	cardiac fibrosis	386:401	cardiac fibrosis	386:401	Therefore, this study was conducted to examine the dynamic changes of B10 cell frequency and their potential role in cardiac fibrosis.
34013598	9	19	theme	cardiac	1291:1297	arg1	injury					1299:1304	cardiac injury	1291:1304	cardiac injury	1291:1304	Our results provide new evidence suggesting that B10 cells exert antifibrotic effects by regulating the extracellular matrix composition during cardiac injury, and also highlight that B10 cells may serve as a promising therapeutic candidate for managing cardiac fibrosis-associated disorders.
34013598	6	20	theme	hyaluronan-induced	720:737	arg1	conversion					739:748	hyaluronan-induced conversion	720:748	hyaluronan-induced conversion of M1/M2 Mc	720:760	hyaluronan-induced conversion of M1/M2 Mc was dependent on the size of hyaluronan.
34013598	3	21	theme	dynamic	320:326	arg1	changes					328:334	the dynamic changes	316:334	the dynamic changes of B10 cell frequency	316:356	Therefore, this study was conducted to examine the dynamic changes of B10 cell frequency and their potential role in cardiac fibrosis.
34013598	8	22	theme	cardiac	1131:1137	arg1	injury					1139:1144	fibrosis following cardiac injury	1112:1144	fibrosis following cardiac injury	1112:1144	Adoptive transfer of B10 cells significantly attenuated collagen deposition whereas CD19-/- mice with reduced B10 cells exacerbated fibrosis following cardiac injury.
34013598	7	23	theme	M2	966:967	arg1	phenotype					969:977	the M2 phenotype	962:977	the M2 phenotype	962:977	Low molecular weight hyaluronan promoted the conversion to M1 Mϕ, whereas medium and high molecular weight hyaluronan accelerated Mϕ transdifferentiation into the M2 phenotype.
34013598	0	24	theme	B10	0:2	arg1	cells					4:8	B10 cells	0:8	B10 cells	0:8	B10 cells decrease fibrosis progression following cardiac injury partially by IL-10 production and regulating hyaluronan secretion.
34013598	9	25	theme	fibrosis-associated	1409:1427	arg1	disorders					1429:1437	cardiac fibrosis-associated disorders	1401:1437	cardiac fibrosis-associated disorders	1401:1437	Our results provide new evidence suggesting that B10 cells exert antifibrotic effects by regulating the extracellular matrix composition during cardiac injury, and also highlight that B10 cells may serve as a promising therapeutic candidate for managing cardiac fibrosis-associated disorders.
34013598	5	26	theme	hyaluronan	632:641	arg1	ablation					643:650	hyaluronan ablation	632:650	hyaluronan ablation	632:650	In vivo, hyaluronan ablation or treatment significantly restricted cardiac fibrosis development.
34013598	7	27	theme	M1	862:863	arg1	Mϕ					865:866	M1 Mϕ	862:866	M1 Mϕ	862:866	Low molecular weight hyaluronan promoted the conversion to M1 Mϕ, whereas medium and high molecular weight hyaluronan accelerated Mϕ transdifferentiation into the M2 phenotype.
34013598	0	28	theme	fibrosis	19:26	arg1	progression					28:38	fibrosis progression	19:38	fibrosis progression	19:38	B10 cells decrease fibrosis progression following cardiac injury partially by IL-10 production and regulating hyaluronan secretion.
34013598	6	29	theme	hyaluronan	791:800	arg1	size					783:786	the size	779:786	the size of hyaluronan	779:800	hyaluronan-induced conversion of M1/M2 Mc was dependent on the size of hyaluronan.
34013598	9	30	theme	new	1167:1169	arg1	evidence					1171:1178	new evidence	1167:1178	new evidence suggesting that B10 cells exert antifibrotic effects by regulating the extracellular matrix composition during cardiac injury	1167:1304	Our results provide new evidence suggesting that B10 cells exert antifibrotic effects by regulating the extracellular matrix composition during cardiac injury, and also highlight that B10 cells may serve as a promising therapeutic candidate for managing cardiac fibrosis-associated disorders.
34013598	8	31	with	mice	1072:1075	arg1	cells					1094:1098	reduced B10 cells	1082:1098	reduced B10 cells	1082:1098	Adoptive transfer of B10 cells significantly attenuated collagen deposition whereas CD19-/- mice with reduced B10 cells exacerbated fibrosis following cardiac injury.
34013598	3	32	theme	potential	368:376	arg1	role					378:381	their potential role	362:381	their potential role in cardiac fibrosis	362:401	Therefore, this study was conducted to examine the dynamic changes of B10 cell frequency and their potential role in cardiac fibrosis.
34013598	7	33	theme	weight	817:822	arg1	hyaluronan					824:833	Low molecular weight hyaluronan	803:833	Low molecular weight hyaluronan	803:833	Low molecular weight hyaluronan promoted the conversion to M1 Mϕ, whereas medium and high molecular weight hyaluronan accelerated Mϕ transdifferentiation into the M2 phenotype.
34013598	4	34	theme	B10	435:437	arg1	cells					439:443	B10 cells	435:443	B10 cells	435:443	We found that the frequency of B10 cells was significantly increased, and they participated in the regression of fibrosis via IL-10, particularly by accelerating hyaluronan secretion and inhibiting collagen deposition.
34013598	7	35	theme	Low	803:805	arg1	weight					817:822	Low molecular weight	803:822	Low molecular weight hyaluronan	803:833	Low molecular weight hyaluronan promoted the conversion to M1 Mϕ, whereas medium and high molecular weight hyaluronan accelerated Mϕ transdifferentiation into the M2 phenotype.
34013598	8	36	theme	CD19-/-	1064:1070	arg1	mice					1072:1075	CD19-/- mice	1064:1075	CD19-/- mice with reduced B10 cells	1064:1098	Adoptive transfer of B10 cells significantly attenuated collagen deposition whereas CD19-/- mice with reduced B10 cells exacerbated fibrosis following cardiac injury.
34013598	3	37	theme	cell	343:346	arg1	frequency					348:356	B10 cell frequency	339:356	B10 cell frequency	339:356	Therefore, this study was conducted to examine the dynamic changes of B10 cell frequency and their potential role in cardiac fibrosis.
34013598	3	38	theme	cardiac	386:392	arg1	fibrosis					394:401	cardiac fibrosis	386:401	cardiac fibrosis	386:401	Therefore, this study was conducted to examine the dynamic changes of B10 cell frequency and their potential role in cardiac fibrosis.
34013598	7	39	theme	molecular	807:815	arg1	weight					817:822	Low molecular weight	803:822	Low molecular weight hyaluronan	803:833	Low molecular weight hyaluronan promoted the conversion to M1 Mϕ, whereas medium and high molecular weight hyaluronan accelerated Mϕ transdifferentiation into the M2 phenotype.
34013598	9	40	theme	matrix	1265:1270	arg1	composition					1272:1282	the extracellular matrix composition	1247:1282	the extracellular matrix composition during cardiac injury	1247:1304	Our results provide new evidence suggesting that B10 cells exert antifibrotic effects by regulating the extracellular matrix composition during cardiac injury, and also highlight that B10 cells may serve as a promising therapeutic candidate for managing cardiac fibrosis-associated disorders.
34013598	4	41	theme	cells	439:443	arg1	frequency					422:430	the frequency	418:430	the frequency of B10 cells	418:443	We found that the frequency of B10 cells was significantly increased, and they participated in the regression of fibrosis via IL-10, particularly by accelerating hyaluronan secretion and inhibiting collagen deposition.
34013598	9	42	theme	B10	1196:1198	arg1	cells					1200:1204	B10 cells	1196:1204	B10 cells	1196:1204	Our results provide new evidence suggesting that B10 cells exert antifibrotic effects by regulating the extracellular matrix composition during cardiac injury, and also highlight that B10 cells may serve as a promising therapeutic candidate for managing cardiac fibrosis-associated disorders.
34013598	0	43	theme	cardiac	50:56	arg1	injury					58:63	cardiac injury	50:63	cardiac injury	50:63	B10 cells decrease fibrosis progression following cardiac injury partially by IL-10 production and regulating hyaluronan secretion.
34013598	5	44	theme	cardiac	690:696	arg1	development					707:717	cardiac fibrosis development	690:717	cardiac fibrosis development	690:717	In vivo, hyaluronan ablation or treatment significantly restricted cardiac fibrosis development.
34013598	9	45	theme	B10	1331:1333	arg1	cells					1335:1339	B10 cells	1331:1339	B10 cells	1331:1339	Our results provide new evidence suggesting that B10 cells exert antifibrotic effects by regulating the extracellular matrix composition during cardiac injury, and also highlight that B10 cells may serve as a promising therapeutic candidate for managing cardiac fibrosis-associated disorders.
34013598	9	45	theme	B10	1331:1333	arg1	candidate					1378:1386	a promising therapeutic candidate	1354:1386	a promising therapeutic candidate for managing cardiac fibrosis-associated disorders	1354:1437	Our results provide new evidence suggesting that B10 cells exert antifibrotic effects by regulating the extracellular matrix composition during cardiac injury, and also highlight that B10 cells may serve as a promising therapeutic candidate for managing cardiac fibrosis-associated disorders.
34013598	7	46	dep	medium	877:882	arg1	hyaluronan					910:919	hyaluronan	910:919	hyaluronan	910:919	Low molecular weight hyaluronan promoted the conversion to M1 Mϕ, whereas medium and high molecular weight hyaluronan accelerated Mϕ transdifferentiation into the M2 phenotype.
34013598	1	47	theme	B10	132:134	arg1	cells					136:140	B10 cells	132:140	B10 cells	132:140	B10 cells play negative roles in inflammatory disorders by producing IL-10.
34013598	4	48	theme	fibrosis	517:524	arg1	regression					503:512	the regression	499:512	the regression of fibrosis	499:524	We found that the frequency of B10 cells was significantly increased, and they participated in the regression of fibrosis via IL-10, particularly by accelerating hyaluronan secretion and inhibiting collagen deposition.
34013598	5	49	theme	fibrosis	698:705	arg1	development					707:717	cardiac fibrosis development	690:717	cardiac fibrosis development	690:717	In vivo, hyaluronan ablation or treatment significantly restricted cardiac fibrosis development.
34013598	9	50	theme	extracellular	1251:1263	arg1	composition					1272:1282	the extracellular matrix composition	1247:1282	the extracellular matrix composition during cardiac injury	1247:1304	Our results provide new evidence suggesting that B10 cells exert antifibrotic effects by regulating the extracellular matrix composition during cardiac injury, and also highlight that B10 cells may serve as a promising therapeutic candidate for managing cardiac fibrosis-associated disorders.
34013598	8	51	theme	B10	1001:1003	arg1	cells					1005:1009	B10 cells	1001:1009	B10 cells	1001:1009	Adoptive transfer of B10 cells significantly attenuated collagen deposition whereas CD19-/- mice with reduced B10 cells exacerbated fibrosis following cardiac injury.
34013598	4	52	theme	collagen	602:609	arg1	deposition					611:620	collagen deposition	602:620	collagen deposition	602:620	We found that the frequency of B10 cells was significantly increased, and they participated in the regression of fibrosis via IL-10, particularly by accelerating hyaluronan secretion and inhibiting collagen deposition.
34013598	3	53	from	changes	328:334	arg1	fibrosis					394:401	cardiac fibrosis	386:401	cardiac fibrosis	386:401	Therefore, this study was conducted to examine the dynamic changes of B10 cell frequency and their potential role in cardiac fibrosis.
34013598	9	54	theme	antifibrotic	1212:1223	arg1	effects					1225:1231	antifibrotic effects	1212:1231	antifibrotic effects	1212:1231	Our results provide new evidence suggesting that B10 cells exert antifibrotic effects by regulating the extracellular matrix composition during cardiac injury, and also highlight that B10 cells may serve as a promising therapeutic candidate for managing cardiac fibrosis-associated disorders.
34013598	8	55	theme	Adoptive	980:987	arg1	transfer					989:996	Adoptive transfer	980:996	Adoptive transfer of B10 cells	980:1009	Adoptive transfer of B10 cells significantly attenuated collagen deposition whereas CD19-/- mice with reduced B10 cells exacerbated fibrosis following cardiac injury.
34013598	1	56	theme	negative	147:154	arg1	roles					156:160	negative roles	147:160	negative roles	147:160	B10 cells play negative roles in inflammatory disorders by producing IL-10.
34013598	7	57	theme	Mϕ	933:934	arg1	transdifferentiation					936:955	Mϕ transdifferentiation	933:955	Mϕ transdifferentiation into the M2 phenotype	933:977	Low molecular weight hyaluronan promoted the conversion to M1 Mϕ, whereas medium and high molecular weight hyaluronan accelerated Mϕ transdifferentiation into the M2 phenotype.
34013598	6	58	theme	Mc	759:760	arg1	conversion					739:748	hyaluronan-induced conversion	720:748	hyaluronan-induced conversion of M1/M2 Mc	720:760	hyaluronan-induced conversion of M1/M2 Mc was dependent on the size of hyaluronan.
34013598	9	59	theme	cardiac	1401:1407	arg1	disorders					1429:1437	cardiac fibrosis-associated disorders	1401:1437	cardiac fibrosis-associated disorders	1401:1437	Our results provide new evidence suggesting that B10 cells exert antifibrotic effects by regulating the extracellular matrix composition during cardiac injury, and also highlight that B10 cells may serve as a promising therapeutic candidate for managing cardiac fibrosis-associated disorders.
34957191	8	0	theme	lactobacilli	1063:1074	arg1	growth					1053:1058	the growth	1049:1058	the growth of lactobacilli	1049:1074	GPs and GOS had a stronger resistance to acid conditions in human stomach than fructooligosaccharide, and GOs showed better prebiotic properties on the growth of lactobacilli than GPs.
34957191	5	1	theme	×	622:622	arg1	Da					628:629	6.57 × 103 Da	617:629	6.57 × 103 Da	617:629	GPs were 6.57 × 103 Da with a composition of fructose and glucose at a ratio of 4:1.
34957191	7	2	theme	oligosaccharides	834:849	arg1	content					823:829	The content	819:829	The content of oligosaccharides with an average DP < 10	819:873	The content of oligosaccharides with an average DP < 10 increased from 15 to 75%.
34957191	0	3	theme	Mixtures	80:87	arg1	Properties					19:28	In vitro Prebiotic Properties	0:28	In vitro Prebiotic Properties of Garlic Polysaccharides and Its Oligosaccharide Mixtures	0:87	In vitro Prebiotic Properties of Garlic Polysaccharides and Its Oligosaccharide Mixtures Obtained by Acid Hydrolysis.
34957191	4	4	theme	polysaccharides	557:571	arg1	potential					525:533	the prebiotic potential	511:533	the prebiotic potential of the garlic neutral polysaccharides (GPs)	511:577	The objective of this study was to extract and purify polysaccharides from garlic, analyze its composition, hydrolyze them using HCl, and then evaluate the prebiotic potential of the garlic neutral polysaccharides (GPs) before and after hydrolysis.
34957191	0	5	theme	Acid	101:104	arg1	Hydrolysis					106:115	Acid Hydrolysis	101:115	Acid Hydrolysis	101:115	In vitro Prebiotic Properties of Garlic Polysaccharides and Its Oligosaccharide Mixtures Obtained by Acid Hydrolysis.
34957191	7	6	with	oligosaccharides	834:849	arg1	DP					867:868	an average DP	856:868	an average DP < 10	856:873	The content of oligosaccharides with an average DP < 10 increased from 15 to 75%.
34957191	9	7	dep	frutctans	1145:1153	arg1	mixtures					1176:1183	mixtures	1176:1183	mixtures	1176:1183	This study evaluates the prebiotic potential of the garlic frutctans and oligosaccharides mixtures obtained by acid hydrolysis, which may be used as an ingredient in functional food and nutraceutical products.
34957191	9	7	dep	frutctans	1145:1153	arg1	the					1134:1136	the	1134:1136	the	1134:1136	This study evaluates the prebiotic potential of the garlic frutctans and oligosaccharides mixtures obtained by acid hydrolysis, which may be used as an ingredient in functional food and nutraceutical products.
34957191	9	8	used	used	1227:1230	arg2	hydrolysis					1202:1211	acid hydrolysis	1197:1211	acid hydrolysis	1197:1211	This study evaluates the prebiotic potential of the garlic frutctans and oligosaccharides mixtures obtained by acid hydrolysis, which may be used as an ingredient in functional food and nutraceutical products.
34957191	9	8	used	used	1227:1230	arg2	ingredient					1238:1247	an ingredient	1235:1247	an ingredient in functional food and nutraceutical products	1235:1293	This study evaluates the prebiotic potential of the garlic frutctans and oligosaccharides mixtures obtained by acid hydrolysis, which may be used as an ingredient in functional food and nutraceutical products.
34957191	6	9	theme	garlic	749:754	arg1	GOs					771:773	GOs	771:773	GOs	771:773	After acid hydrolysis, low molecular weight fraction in garlic oligofructose (GOs) may be eliminated through ultrafiltration.
34957191	6	9	theme	garlic	749:754	arg1	oligofructose					756:768	garlic oligofructose	749:768	garlic oligofructose (GOs)	749:774	After acid hydrolysis, low molecular weight fraction in garlic oligofructose (GOs) may be eliminated through ultrafiltration.
34957191	2	10	theme	polysaccharides	276:290	arg1	polymerization					253:266	polymerization	253:266	polymerization (DP) of polysaccharides	253:290	The degree of polymerization (DP) of polysaccharides affects the utilization of probiotics.
34957191	2	10	theme	polysaccharides	276:290	arg1	DP					269:270	DP	269:270	DP	269:270	The degree of polymerization (DP) of polysaccharides affects the utilization of probiotics.
34957191	5	11	theme	4:1	688:690	arg1	ratio					679:683	a ratio	677:683	a ratio of 4:1	677:690	GPs were 6.57 × 103 Da with a composition of fructose and glucose at a ratio of 4:1.
34957191	9	12	theme	prebiotic	1111:1119	arg1	potential					1121:1129	the prebiotic potential	1107:1129	the prebiotic potential of the garlic frutctans and oligosaccharides mixtures obtained by acid hydrolysis, which may be used as an ingredient in functional food and nutraceutical products	1107:1293	This study evaluates the prebiotic potential of the garlic frutctans and oligosaccharides mixtures obtained by acid hydrolysis, which may be used as an ingredient in functional food and nutraceutical products.
34957191	1	13	used	used	157:160	arg2	prebiotics					165:174	prebiotics	165:174	prebiotics without any limitation in functional food or food ingredients	165:236	Fructans and oligofructose are usually used as prebiotics without any limitation in functional food or food ingredients.
34957191	1	13	used	used	157:160	arg2	Fructans					118:125	Fructans	118:125	Fructans	118:125	Fructans and oligofructose are usually used as prebiotics without any limitation in functional food or food ingredients.
34957191	1	13	used	used	157:160	arg2	oligofructose					131:143	oligofructose	131:143	oligofructose	131:143	Fructans and oligofructose are usually used as prebiotics without any limitation in functional food or food ingredients.
34957191	8	14	theme	human	961:965	arg1	stomach					967:973	human stomach	961:973	human stomach	961:973	GPs and GOS had a stronger resistance to acid conditions in human stomach than fructooligosaccharide, and GOs showed better prebiotic properties on the growth of lactobacilli than GPs.
34957191	9	15	from	ingredient	1238:1247	arg1	food					1263:1266	functional food	1252:1266	functional food	1252:1266	This study evaluates the prebiotic potential of the garlic frutctans and oligosaccharides mixtures obtained by acid hydrolysis, which may be used as an ingredient in functional food and nutraceutical products.
34957191	9	15	from	ingredient	1238:1247	arg1	products					1286:1293	nutraceutical products	1272:1293	nutraceutical products	1272:1293	This study evaluates the prebiotic potential of the garlic frutctans and oligosaccharides mixtures obtained by acid hydrolysis, which may be used as an ingredient in functional food and nutraceutical products.
34957191	6	16	theme	weight	730:735	arg1	fraction					737:744	low molecular weight fraction	716:744	low molecular weight fraction in garlic oligofructose (GOs)	716:774	After acid hydrolysis, low molecular weight fraction in garlic oligofructose (GOs) may be eliminated through ultrafiltration.
34957191	2	17	theme	probiotics	319:328	arg1	utilization					304:314	the utilization	300:314	the utilization of probiotics	300:328	The degree of polymerization (DP) of polysaccharides affects the utilization of probiotics.
34957191	0	18	theme	In	0:1	arg1	Properties					19:28	In vitro Prebiotic Properties	0:28	In vitro Prebiotic Properties of Garlic Polysaccharides and Its Oligosaccharide Mixtures	0:87	In vitro Prebiotic Properties of Garlic Polysaccharides and Its Oligosaccharide Mixtures Obtained by Acid Hydrolysis.
34957191	5	19	theme	6.57	617:620	arg1	Da					628:629	6.57 × 103 Da	617:629	6.57 × 103 Da	617:629	GPs were 6.57 × 103 Da with a composition of fructose and glucose at a ratio of 4:1.
34957191	4	20	theme	study	381:385	arg1	objective					363:371	The objective	359:371	The objective of this study	359:385	The objective of this study was to extract and purify polysaccharides from garlic, analyze its composition, hydrolyze them using HCl, and then evaluate the prebiotic potential of the garlic neutral polysaccharides (GPs) before and after hydrolysis.
34957191	6	21	theme	molecular	720:728	arg1	fraction					737:744	low molecular weight fraction	716:744	low molecular weight fraction in garlic oligofructose (GOs)	716:774	After acid hydrolysis, low molecular weight fraction in garlic oligofructose (GOs) may be eliminated through ultrafiltration.
34957191	6	22	theme	low	716:718	arg1	fraction					737:744	low molecular weight fraction	716:744	low molecular weight fraction in garlic oligofructose (GOs)	716:774	After acid hydrolysis, low molecular weight fraction in garlic oligofructose (GOs) may be eliminated through ultrafiltration.
34957191	1	23	theme	functional	202:211	arg1	food					213:216	functional food	202:216	functional food	202:216	Fructans and oligofructose are usually used as prebiotics without any limitation in functional food or food ingredients.
34957191	0	24	theme	Prebiotic	9:17	arg1	Properties					19:28	In vitro Prebiotic Properties	0:28	In vitro Prebiotic Properties of Garlic Polysaccharides and Its Oligosaccharide Mixtures	0:87	In vitro Prebiotic Properties of Garlic Polysaccharides and Its Oligosaccharide Mixtures Obtained by Acid Hydrolysis.
34957191	4	25	theme	neutral	549:555	arg1	GPs					574:576	GPs	574:576	GPs	574:576	The objective of this study was to extract and purify polysaccharides from garlic, analyze its composition, hydrolyze them using HCl, and then evaluate the prebiotic potential of the garlic neutral polysaccharides (GPs) before and after hydrolysis.
34957191	4	25	theme	neutral	549:555	arg1	polysaccharides					557:571	the garlic neutral polysaccharides	538:571	the garlic neutral polysaccharides (GPs)	538:577	The objective of this study was to extract and purify polysaccharides from garlic, analyze its composition, hydrolyze them using HCl, and then evaluate the prebiotic potential of the garlic neutral polysaccharides (GPs) before and after hydrolysis.
34957191	5	26	theme	glucose	666:672	arg1	composition					638:648	a composition	636:648	a composition of fructose and glucose	636:672	GPs were 6.57 × 103 Da with a composition of fructose and glucose at a ratio of 4:1.
34957191	9	27	theme	frutctans	1145:1153	arg1	potential					1121:1129	the prebiotic potential	1107:1129	the prebiotic potential of the garlic frutctans and oligosaccharides mixtures obtained by acid hydrolysis, which may be used as an ingredient in functional food and nutraceutical products	1107:1293	This study evaluates the prebiotic potential of the garlic frutctans and oligosaccharides mixtures obtained by acid hydrolysis, which may be used as an ingredient in functional food and nutraceutical products.
34957191	8	28	from	resistance	928:937	arg1	stomach					967:973	human stomach	961:973	human stomach	961:973	GPs and GOS had a stronger resistance to acid conditions in human stomach than fructooligosaccharide, and GOs showed better prebiotic properties on the growth of lactobacilli than GPs.
34957191	0	29	theme	Garlic	33:38	arg1	Polysaccharides					40:54	Garlic Polysaccharides	33:54	Garlic Polysaccharides	33:54	In vitro Prebiotic Properties of Garlic Polysaccharides and Its Oligosaccharide Mixtures Obtained by Acid Hydrolysis.
34957191	4	30	theme	garlic	542:547	arg1	GPs					574:576	GPs	574:576	GPs	574:576	The objective of this study was to extract and purify polysaccharides from garlic, analyze its composition, hydrolyze them using HCl, and then evaluate the prebiotic potential of the garlic neutral polysaccharides (GPs) before and after hydrolysis.
34957191	4	30	theme	garlic	542:547	arg1	polysaccharides					557:571	the garlic neutral polysaccharides	538:571	the garlic neutral polysaccharides (GPs)	538:577	The objective of this study was to extract and purify polysaccharides from garlic, analyze its composition, hydrolyze them using HCl, and then evaluate the prebiotic potential of the garlic neutral polysaccharides (GPs) before and after hydrolysis.
34957191	8	31	theme	prebiotic	1025:1033	arg1	properties					1035:1044	better prebiotic properties	1018:1044	better prebiotic properties	1018:1044	GPs and GOS had a stronger resistance to acid conditions in human stomach than fructooligosaccharide, and GOs showed better prebiotic properties on the growth of lactobacilli than GPs.
34957191	3	32	from	fructans	349:356	arg1	rich					341:344	rich	341:344	rich	341:344	Garlic is rich in fructans.
34957191	6	33	from	fraction	737:744	arg1	GOs					771:773	GOs	771:773	GOs	771:773	After acid hydrolysis, low molecular weight fraction in garlic oligofructose (GOs) may be eliminated through ultrafiltration.
34957191	6	33	from	fraction	737:744	arg1	oligofructose					756:768	garlic oligofructose	749:768	garlic oligofructose (GOs)	749:774	After acid hydrolysis, low molecular weight fraction in garlic oligofructose (GOs) may be eliminated through ultrafiltration.
34957191	9	34	theme	nutraceutical	1272:1284	arg1	products					1286:1293	nutraceutical products	1272:1293	nutraceutical products	1272:1293	This study evaluates the prebiotic potential of the garlic frutctans and oligosaccharides mixtures obtained by acid hydrolysis, which may be used as an ingredient in functional food and nutraceutical products.
34957191	5	35	from	ratio	679:683	arg1	Da					628:629	6.57 × 103 Da	617:629	6.57 × 103 Da	617:629	GPs were 6.57 × 103 Da with a composition of fructose and glucose at a ratio of 4:1.
34957191	8	36	theme	stronger	919:926	arg1	resistance					928:937	a stronger resistance	917:937	a stronger resistance to acid conditions in human stomach	917:973	GPs and GOS had a stronger resistance to acid conditions in human stomach than fructooligosaccharide, and GOs showed better prebiotic properties on the growth of lactobacilli than GPs.
34957191	1	37	theme	food	221:224	arg1	ingredients					226:236	food ingredients	221:236	food ingredients	221:236	Fructans and oligofructose are usually used as prebiotics without any limitation in functional food or food ingredients.
34957191	4	38	from	garlic	434:439	arg1	polysaccharides					413:427	polysaccharides	413:427	polysaccharides from garlic	413:439	The objective of this study was to extract and purify polysaccharides from garlic, analyze its composition, hydrolyze them using HCl, and then evaluate the prebiotic potential of the garlic neutral polysaccharides (GPs) before and after hydrolysis.
34957191	9	39	theme	functional	1252:1261	arg1	food					1263:1266	functional food	1252:1266	functional food	1252:1266	This study evaluates the prebiotic potential of the garlic frutctans and oligosaccharides mixtures obtained by acid hydrolysis, which may be used as an ingredient in functional food and nutraceutical products.
34957191	9	40	theme	garlic	1138:1143	arg1	frutctans					1145:1153	garlic frutctans	1138:1153	garlic frutctans	1138:1153	This study evaluates the prebiotic potential of the garlic frutctans and oligosaccharides mixtures obtained by acid hydrolysis, which may be used as an ingredient in functional food and nutraceutical products.
34957191	0	41	theme	Polysaccharides	40:54	arg1	Properties					19:28	In vitro Prebiotic Properties	0:28	In vitro Prebiotic Properties of Garlic Polysaccharides and Its Oligosaccharide Mixtures	0:87	In vitro Prebiotic Properties of Garlic Polysaccharides and Its Oligosaccharide Mixtures Obtained by Acid Hydrolysis.
34957191	8	42	theme	better	1018:1023	arg1	properties					1035:1044	better prebiotic properties	1018:1044	better prebiotic properties	1018:1044	GPs and GOS had a stronger resistance to acid conditions in human stomach than fructooligosaccharide, and GOs showed better prebiotic properties on the growth of lactobacilli than GPs.
34957191	8	43	theme	acid	942:945	arg1	conditions					947:956	acid conditions	942:956	acid conditions	942:956	GPs and GOS had a stronger resistance to acid conditions in human stomach than fructooligosaccharide, and GOs showed better prebiotic properties on the growth of lactobacilli than GPs.
34957191	6	44	theme	acid	699:702	arg1	hydrolysis					704:713	acid hydrolysis	699:713	acid hydrolysis	699:713	After acid hydrolysis, low molecular weight fraction in garlic oligofructose (GOs) may be eliminated through ultrafiltration.
34957191	9	45	theme	oligosaccharides	1159:1174	arg1	potential					1121:1129	the prebiotic potential	1107:1129	the prebiotic potential of the garlic frutctans and oligosaccharides mixtures obtained by acid hydrolysis, which may be used as an ingredient in functional food and nutraceutical products	1107:1293	This study evaluates the prebiotic potential of the garlic frutctans and oligosaccharides mixtures obtained by acid hydrolysis, which may be used as an ingredient in functional food and nutraceutical products.
34957191	7	46	theme	average	859:865	arg1	DP					867:868	an average DP	856:868	an average DP < 10	856:873	The content of oligosaccharides with an average DP < 10 increased from 15 to 75%.
34957191	4	47	theme	prebiotic	515:523	arg1	potential					525:533	the prebiotic potential	511:533	the prebiotic potential of the garlic neutral polysaccharides (GPs)	511:577	The objective of this study was to extract and purify polysaccharides from garlic, analyze its composition, hydrolyze them using HCl, and then evaluate the prebiotic potential of the garlic neutral polysaccharides (GPs) before and after hydrolysis.
34957191	5	48	theme	fructose	653:660	arg1	composition					638:648	a composition	636:648	a composition of fructose and glucose	636:672	GPs were 6.57 × 103 Da with a composition of fructose and glucose at a ratio of 4:1.
34957191	9	49	theme	acid	1197:1200	arg1	ingredient					1238:1247	an ingredient	1235:1247	an ingredient in functional food and nutraceutical products	1235:1293	This study evaluates the prebiotic potential of the garlic frutctans and oligosaccharides mixtures obtained by acid hydrolysis, which may be used as an ingredient in functional food and nutraceutical products.
34957191	9	49	theme	acid	1197:1200	arg1	hydrolysis					1202:1211	acid hydrolysis	1197:1211	acid hydrolysis	1197:1211	This study evaluates the prebiotic potential of the garlic frutctans and oligosaccharides mixtures obtained by acid hydrolysis, which may be used as an ingredient in functional food and nutraceutical products.
34957191	1	50	from	limitation	188:197	arg1	food					213:216	functional food	202:216	functional food	202:216	Fructans and oligofructose are usually used as prebiotics without any limitation in functional food or food ingredients.
34957191	1	50	from	limitation	188:197	arg1	ingredients					226:236	food ingredients	221:236	food ingredients	221:236	Fructans and oligofructose are usually used as prebiotics without any limitation in functional food or food ingredients.
34957191	7	51	dep	75	896:897	arg1	to					893:894	to	893:894	to	893:894	The content of oligosaccharides with an average DP < 10 increased from 15 to 75%.
34957191	0	52	theme	Oligosaccharide	64:78	arg1	Mixtures					80:87	Its Oligosaccharide Mixtures	60:87	Its Oligosaccharide Mixtures	60:87	In vitro Prebiotic Properties of Garlic Polysaccharides and Its Oligosaccharide Mixtures Obtained by Acid Hydrolysis.
34957191	3	53	from	rich	341:344	arg1	fructans					349:356	fructans	349:356	fructans	349:356	Garlic is rich in fructans.
34957191	2	54	theme	polymerization	253:266	arg1	degree					243:248	The degree	239:248	The degree of polymerization (DP) of polysaccharides	239:290	The degree of polymerization (DP) of polysaccharides affects the utilization of probiotics.
34957191	5	55	with	Da	628:629	arg1	composition					638:648	a composition	636:648	a composition of fructose and glucose	636:672	GPs were 6.57 × 103 Da with a composition of fructose and glucose at a ratio of 4:1.
34957191	8	56	contain	had	913:915	arg2	resistance					928:937	a stronger resistance	917:937	a stronger resistance to acid conditions in human stomach	917:973	GPs and GOS had a stronger resistance to acid conditions in human stomach than fructooligosaccharide, and GOs showed better prebiotic properties on the growth of lactobacilli than GPs.
34957191	8	56	contain	had	913:915	arg1	GOS					909:911	GOS	909:911	GOS	909:911	GPs and GOS had a stronger resistance to acid conditions in human stomach than fructooligosaccharide, and GOs showed better prebiotic properties on the growth of lactobacilli than GPs.
34957191	8	56	contain	had	913:915	arg1	GPs					901:903	GPs	901:903	GPs	901:903	GPs and GOS had a stronger resistance to acid conditions in human stomach than fructooligosaccharide, and GOs showed better prebiotic properties on the growth of lactobacilli than GPs.
34957191	0	57	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro Prebiotic Properties of Garlic Polysaccharides and Its Oligosaccharide Mixtures Obtained by Acid Hydrolysis.
34118572	0	0	theme	mixed-herb	118:127	arg1	decoction					129:137	mixed-herb decoction	118:137	mixed-herb decoction	118:137	Integrating multiple-chromatographic approaches to evaluate chemical consistency of Chang-Kang-Fang preparations from mixed-herb decoction and combined single-herb decoction.
34118572	6	1	theme	CKF	1746:1748	arg1	efficacy					1723:1730	efficacy	1723:1730	efficacy	1723:1730	Whether or not these quality variations affect the efficacy and safety of CKF deserves further investigation.
34118572	6	1	theme	CKF	1746:1748	arg1	safety					1736:1741	safety	1736:1741	safety	1736:1741	Whether or not these quality variations affect the efficacy and safety of CKF deserves further investigation.
34118572	3	2	theme	saccharides	1017:1027	arg1	content					1029:1035	total saccharides content	1011:1035	total saccharides content	1011:1035	Sixteen small molecules were simultaneously qualified and quantified by UPLC-QTOF-MS/MS, the molecular weight distribution of polysaccharides was characterized by HPGPC-ELSD, while the monosaccharide composition and total saccharides content were determined by HPLC-PDA and UV-VIS, respectively.
34118572	5	3	theme	paeoniflorin	1358:1369	arg1	lower					1397:1401	lower	1397:1401	lower	1397:1401	However, the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD were significantly lower than those in cSHD, whereas the content of polysaccharides in MHD was higher than that in cSHD, indicating that there is a significant difference in the quality between MHD and cSHD, in particular for the relative contents of major small molecules and polysaccharides.
34118572	5	3	theme	paeoniflorin	1358:1369	arg1	contents					1284:1291	the contents	1280:1291	the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD	1280:1376	However, the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD were significantly lower than those in cSHD, whereas the content of polysaccharides in MHD was higher than that in cSHD, indicating that there is a significant difference in the quality between MHD and cSHD, in particular for the relative contents of major small molecules and polysaccharides.
34118572	1	4	theme	bowel	291:295	arg1	syndrome					297:304	irritable bowel syndrome	281:304	irritable bowel syndrome (IBS)	281:310	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	1	4	theme	bowel	291:295	arg1	IBS					307:309	IBS	307:309	IBS	307:309	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	1	5	from	form	413:416	arg1	decades					511:517	the near decades	502:517	the near decades	502:517	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	1	6	theme	single-herb	430:440	arg1	cSHD					453:456	cSHD	453:456	cSHD	453:456	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	1	6	theme	single-herb	430:440	arg1	decoction					442:450	combined single-herb decoction	421:450	combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades	421:517	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	1	7	from	decoction	442:450	arg1	decades					511:517	the near decades	502:517	the near decades	502:517	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	4	8	theme	molecules	1223:1231	arg1	composition					1202:1212	the composition	1198:1212	the composition of small molecules	1198:1231	It was found that the molecular weight range and monosaccharide composition of polysaccharides, as well as the composition of small molecules, were identical between MHD and cSHD.
34118572	4	8	theme	molecules	1223:1231	arg1	polysaccharides					1170:1184	polysaccharides	1170:1184	polysaccharides	1170:1184	It was found that the molecular weight range and monosaccharide composition of polysaccharides, as well as the composition of small molecules, were identical between MHD and cSHD.
34118572	5	9	from	contents	1284:1291	arg1	MHD					1374:1376	MHD	1374:1376	MHD	1374:1376	However, the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD were significantly lower than those in cSHD, whereas the content of polysaccharides in MHD was higher than that in cSHD, indicating that there is a significant difference in the quality between MHD and cSHD, in particular for the relative contents of major small molecules and polysaccharides.
34118572	1	10	theme	mixed-herb	368:377	arg1	MHD					390:392	MHD	390:392	MHD	390:392	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	1	10	theme	mixed-herb	368:377	arg1	decoction					379:387	mixed-herb decoction	368:387	mixed-herb decoction (MHD)	368:393	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	1	11	theme	decoction	442:450	arg1	form					413:416	the modern form	402:416	the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades	402:517	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	3	12	theme	molecular	888:896	arg1	distribution					905:916	the molecular weight distribution	884:916	the molecular weight distribution of polysaccharides	884:935	Sixteen small molecules were simultaneously qualified and quantified by UPLC-QTOF-MS/MS, the molecular weight distribution of polysaccharides was characterized by HPGPC-ELSD, while the monosaccharide composition and total saccharides content were determined by HPLC-PDA and UV-VIS, respectively.
34118572	5	13	from	difference	1538:1547	arg1	quality					1556:1562	the quality	1552:1562	the quality	1552:1562	However, the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD were significantly lower than those in cSHD, whereas the content of polysaccharides in MHD was higher than that in cSHD, indicating that there is a significant difference in the quality between MHD and cSHD, in particular for the relative contents of major small molecules and polysaccharides.
34118572	1	14	theme	decoction	379:387	arg1	form					360:363	the traditional form	344:363	the traditional form of mixed-herb decoction (MHD)	344:393	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	0	15	theme	single-herb	152:162	arg1	decoction					164:172	combined single-herb decoction	143:172	combined single-herb decoction	143:172	Integrating multiple-chromatographic approaches to evaluate chemical consistency of Chang-Kang-Fang preparations from mixed-herb decoction and combined single-herb decoction.
34118572	0	16	from	consistency	69:79	arg1	decoction					164:172	combined single-herb decoction	143:172	combined single-herb decoction	143:172	Integrating multiple-chromatographic approaches to evaluate chemical consistency of Chang-Kang-Fang preparations from mixed-herb decoction and combined single-herb decoction.
34118572	0	16	from	consistency	69:79	arg1	decoction					129:137	mixed-herb decoction	118:137	mixed-herb decoction	118:137	Integrating multiple-chromatographic approaches to evaluate chemical consistency of Chang-Kang-Fang preparations from mixed-herb decoction and combined single-herb decoction.
34118572	2	17	theme	new	602:604	arg1	strategy					606:613	a new strategy	600:613	a new strategy by integrating multiple-chromatographic approaches to characterize both polysaccharides and small molecules	600:721	Herein, a new strategy by integrating multiple-chromatographic approaches to characterize both polysaccharides and small molecules was developed to compare the chemical consistency between MHD and cSHD.
34118572	2	18	theme	chemical	752:759	arg1	consistency					761:771	the chemical consistency	748:771	the chemical consistency between MHD and cSHD	748:792	Herein, a new strategy by integrating multiple-chromatographic approaches to characterize both polysaccharides and small molecules was developed to compare the chemical consistency between MHD and cSHD.
34118572	1	19	from	decades	511:517	arg1	form					413:416	the modern form	402:416	the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades	402:517	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	0	20	theme	combined	143:150	arg1	decoction					164:172	combined single-herb decoction	143:172	combined single-herb decoction	143:172	Integrating multiple-chromatographic approaches to evaluate chemical consistency of Chang-Kang-Fang preparations from mixed-herb decoction and combined single-herb decoction.
34118572	4	21	dep	range	1130:1134	arg1	the					1109:1111	the	1109:1111	the	1109:1111	It was found that the molecular weight range and monosaccharide composition of polysaccharides, as well as the composition of small molecules, were identical between MHD and cSHD.
34118572	5	22	theme	epiberberine	1307:1318	arg1	lower					1397:1401	lower	1397:1401	lower	1397:1401	However, the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD were significantly lower than those in cSHD, whereas the content of polysaccharides in MHD was higher than that in cSHD, indicating that there is a significant difference in the quality between MHD and cSHD, in particular for the relative contents of major small molecules and polysaccharides.
34118572	5	22	theme	epiberberine	1307:1318	arg1	contents					1284:1291	the contents	1280:1291	the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD	1280:1376	However, the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD were significantly lower than those in cSHD, whereas the content of polysaccharides in MHD was higher than that in cSHD, indicating that there is a significant difference in the quality between MHD and cSHD, in particular for the relative contents of major small molecules and polysaccharides.
34118572	1	23	theme	chemical	528:535	arg1	unknown					583:589	unknown	583:589	unknown	583:589	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	1	23	theme	chemical	528:535	arg1	consistency					537:547	the chemical consistency	524:547	the chemical consistency between the MHD and cSHD	524:572	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	1	24	theme	multi-herbs	208:218	arg1	TCM					250:252	TCM	250:252	TCM	250:252	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	1	24	theme	multi-herbs	208:218	arg1	medicine					240:247	a multi-herbs traditional Chinese medicine	206:247	a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS)	206:310	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	0	25	theme	multiple-chromatographic	12:35	arg1	approaches					37:46	multiple-chromatographic approaches	12:46	multiple-chromatographic approaches	12:46	Integrating multiple-chromatographic approaches to evaluate chemical consistency of Chang-Kang-Fang preparations from mixed-herb decoction and combined single-herb decoction.
34118572	3	26	theme	polysaccharides	921:935	arg1	distribution					905:916	the molecular weight distribution	884:916	the molecular weight distribution of polysaccharides	884:935	Sixteen small molecules were simultaneously qualified and quantified by UPLC-QTOF-MS/MS, the molecular weight distribution of polysaccharides was characterized by HPGPC-ELSD, while the monosaccharide composition and total saccharides content were determined by HPLC-PDA and UV-VIS, respectively.
34118572	1	27	theme	traditional	220:230	arg1	TCM					250:252	TCM	250:252	TCM	250:252	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	1	27	theme	traditional	220:230	arg1	medicine					240:247	a multi-herbs traditional Chinese medicine	206:247	a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS)	206:310	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	5	28	theme	small	1635:1639	arg1	molecules					1641:1649	major small molecules	1629:1649	major small molecules	1629:1649	However, the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD were significantly lower than those in cSHD, whereas the content of polysaccharides in MHD was higher than that in cSHD, indicating that there is a significant difference in the quality between MHD and cSHD, in particular for the relative contents of major small molecules and polysaccharides.
34118572	0	29	from	decoction	129:137	arg1	consistency					69:79	chemical consistency	60:79	chemical consistency of Chang-Kang-Fang preparations from mixed-herb decoction and combined single-herb decoction	60:172	Integrating multiple-chromatographic approaches to evaluate chemical consistency of Chang-Kang-Fang preparations from mixed-herb decoction and combined single-herb decoction.
34118572	0	29	from	decoction	129:137	arg1	preparations					100:111	Chang-Kang-Fang preparations	84:111	Chang-Kang-Fang preparations from mixed-herb decoction and combined single-herb decoction	84:172	Integrating multiple-chromatographic approaches to evaluate chemical consistency of Chang-Kang-Fang preparations from mixed-herb decoction and combined single-herb decoction.
34118572	1	30	theme	called	462:467	arg1	decoction					488:496	so called dispensing granule decoction	459:496	so called dispensing granule decoction	459:496	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	4	31	theme	monosaccharide	1140:1153	arg1	composition					1155:1165	monosaccharide composition	1140:1165	monosaccharide composition	1140:1165	It was found that the molecular weight range and monosaccharide composition of polysaccharides, as well as the composition of small molecules, were identical between MHD and cSHD.
34118572	1	32	theme	Chinese	232:238	arg1	TCM					250:252	TCM	250:252	TCM	250:252	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	1	32	theme	Chinese	232:238	arg1	medicine					240:247	a multi-herbs traditional Chinese medicine	206:247	a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS)	206:310	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	5	33	theme	molecules	1641:1649	arg1	contents					1617:1624	the relative contents	1604:1624	the relative contents of major small molecules and polysaccharides	1604:1669	However, the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD were significantly lower than those in cSHD, whereas the content of polysaccharides in MHD was higher than that in cSHD, indicating that there is a significant difference in the quality between MHD and cSHD, in particular for the relative contents of major small molecules and polysaccharides.
34118572	1	34	theme	dispensing	469:478	arg1	decoction					488:496	so called dispensing granule decoction	459:496	so called dispensing granule decoction	459:496	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	5	35	theme	polysaccharides	1446:1460	arg1	content					1435:1441	the content	1431:1441	the content of polysaccharides in MHD	1431:1467	However, the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD were significantly lower than those in cSHD, whereas the content of polysaccharides in MHD was higher than that in cSHD, indicating that there is a significant difference in the quality between MHD and cSHD, in particular for the relative contents of major small molecules and polysaccharides.
34118572	5	35	theme	polysaccharides	1446:1460	arg1	higher					1473:1478	higher	1473:1478	higher	1473:1478	However, the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD were significantly lower than those in cSHD, whereas the content of polysaccharides in MHD was higher than that in cSHD, indicating that there is a significant difference in the quality between MHD and cSHD, in particular for the relative contents of major small molecules and polysaccharides.
34118572	1	36	theme	medicine	240:247	arg1	prescription					255:266	a multi-herbs traditional Chinese medicine (TCM) prescription	206:266	a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS)	206:310	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	1	36	theme	medicine	240:247	arg1	formula					191:197	Chang-Kang-Fang formula	175:197	Chang-Kang-Fang formula (CKF)	175:203	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	5	37	theme	albiflorin	1343:1352	arg1	lower					1397:1401	lower	1397:1401	lower	1397:1401	However, the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD were significantly lower than those in cSHD, whereas the content of polysaccharides in MHD was higher than that in cSHD, indicating that there is a significant difference in the quality between MHD and cSHD, in particular for the relative contents of major small molecules and polysaccharides.
34118572	5	37	theme	albiflorin	1343:1352	arg1	contents					1284:1291	the contents	1280:1291	the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD	1280:1376	However, the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD were significantly lower than those in cSHD, whereas the content of polysaccharides in MHD was higher than that in cSHD, indicating that there is a significant difference in the quality between MHD and cSHD, in particular for the relative contents of major small molecules and polysaccharides.
34118572	4	38	theme	composition	1202:1212	arg1	composition					1155:1165	monosaccharide composition	1140:1165	monosaccharide composition	1140:1165	It was found that the molecular weight range and monosaccharide composition of polysaccharides, as well as the composition of small molecules, were identical between MHD and cSHD.
34118572	4	38	theme	composition	1202:1212	arg1	range					1130:1134	molecular weight range	1113:1134	molecular weight range	1113:1134	It was found that the molecular weight range and monosaccharide composition of polysaccharides, as well as the composition of small molecules, were identical between MHD and cSHD.
34118572	0	39	theme	chemical	60:67	arg1	consistency					69:79	chemical consistency	60:79	chemical consistency of Chang-Kang-Fang preparations from mixed-herb decoction and combined single-herb decoction	60:172	Integrating multiple-chromatographic approaches to evaluate chemical consistency of Chang-Kang-Fang preparations from mixed-herb decoction and combined single-herb decoction.
34118572	4	40	theme	polysaccharides	1170:1184	arg1	composition					1155:1165	monosaccharide composition	1140:1165	monosaccharide composition	1140:1165	It was found that the molecular weight range and monosaccharide composition of polysaccharides, as well as the composition of small molecules, were identical between MHD and cSHD.
34118572	4	40	theme	polysaccharides	1170:1184	arg1	range					1130:1134	molecular weight range	1113:1134	molecular weight range	1113:1134	It was found that the molecular weight range and monosaccharide composition of polysaccharides, as well as the composition of small molecules, were identical between MHD and cSHD.
34118572	6	41	theme	quality	1693:1699	arg1	variations					1701:1710	these quality variations	1687:1710	these quality variations	1687:1710	Whether or not these quality variations affect the efficacy and safety of CKF deserves further investigation.
34118572	5	42	theme	coptisine	1321:1329	arg1	lower					1397:1401	lower	1397:1401	lower	1397:1401	However, the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD were significantly lower than those in cSHD, whereas the content of polysaccharides in MHD was higher than that in cSHD, indicating that there is a significant difference in the quality between MHD and cSHD, in particular for the relative contents of major small molecules and polysaccharides.
34118572	5	42	theme	coptisine	1321:1329	arg1	contents					1284:1291	the contents	1280:1291	the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD	1280:1376	However, the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD were significantly lower than those in cSHD, whereas the content of polysaccharides in MHD was higher than that in cSHD, indicating that there is a significant difference in the quality between MHD and cSHD, in particular for the relative contents of major small molecules and polysaccharides.
34118572	5	43	theme	relative	1608:1615	arg1	contents					1617:1624	the relative contents	1604:1624	the relative contents of major small molecules and polysaccharides	1604:1669	However, the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD were significantly lower than those in cSHD, whereas the content of polysaccharides in MHD was higher than that in cSHD, indicating that there is a significant difference in the quality between MHD and cSHD, in particular for the relative contents of major small molecules and polysaccharides.
34118572	1	44	theme	granule	480:486	arg1	decoction					488:496	so called dispensing granule decoction	459:496	so called dispensing granule decoction	459:496	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	6	45	theme	further	1759:1765	arg1	investigation					1767:1779	further investigation	1759:1779	further investigation	1759:1779	Whether or not these quality variations affect the efficacy and safety of CKF deserves further investigation.
34118572	5	46	theme	berberine	1296:1304	arg1	lower					1397:1401	lower	1397:1401	lower	1397:1401	However, the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD were significantly lower than those in cSHD, whereas the content of polysaccharides in MHD was higher than that in cSHD, indicating that there is a significant difference in the quality between MHD and cSHD, in particular for the relative contents of major small molecules and polysaccharides.
34118572	5	46	theme	berberine	1296:1304	arg1	contents					1284:1291	the contents	1280:1291	the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD	1280:1376	However, the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD were significantly lower than those in cSHD, whereas the content of polysaccharides in MHD was higher than that in cSHD, indicating that there is a significant difference in the quality between MHD and cSHD, in particular for the relative contents of major small molecules and polysaccharides.
34118572	4	47	theme	small	1217:1221	arg1	molecules					1223:1231	small molecules	1217:1231	small molecules	1217:1231	It was found that the molecular weight range and monosaccharide composition of polysaccharides, as well as the composition of small molecules, were identical between MHD and cSHD.
34118572	5	48	from	content	1435:1441	arg1	MHD					1465:1467	MHD	1465:1467	MHD	1465:1467	However, the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD were significantly lower than those in cSHD, whereas the content of polysaccharides in MHD was higher than that in cSHD, indicating that there is a significant difference in the quality between MHD and cSHD, in particular for the relative contents of major small molecules and polysaccharides.
34118572	1	49	theme	modern	406:411	arg1	form					413:416	the modern form	402:416	the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades	402:517	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	5	50	theme	palmatine	1332:1340	arg1	lower					1397:1401	lower	1397:1401	lower	1397:1401	However, the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD were significantly lower than those in cSHD, whereas the content of polysaccharides in MHD was higher than that in cSHD, indicating that there is a significant difference in the quality between MHD and cSHD, in particular for the relative contents of major small molecules and polysaccharides.
34118572	5	50	theme	palmatine	1332:1340	arg1	contents					1284:1291	the contents	1280:1291	the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD	1280:1376	However, the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD were significantly lower than those in cSHD, whereas the content of polysaccharides in MHD was higher than that in cSHD, indicating that there is a significant difference in the quality between MHD and cSHD, in particular for the relative contents of major small molecules and polysaccharides.
34118572	4	51	theme	molecular	1113:1121	arg1	range					1130:1134	molecular weight range	1113:1134	molecular weight range	1113:1134	It was found that the molecular weight range and monosaccharide composition of polysaccharides, as well as the composition of small molecules, were identical between MHD and cSHD.
34118572	2	52	theme	multiple-chromatographic	630:653	arg1	approaches					655:664	multiple-chromatographic approaches	630:664	multiple-chromatographic approaches	630:664	Herein, a new strategy by integrating multiple-chromatographic approaches to characterize both polysaccharides and small molecules was developed to compare the chemical consistency between MHD and cSHD.
34118572	3	53	theme	monosaccharide	980:993	arg1	composition					995:1005	the monosaccharide composition	976:1005	the monosaccharide composition	976:1005	Sixteen small molecules were simultaneously qualified and quantified by UPLC-QTOF-MS/MS, the molecular weight distribution of polysaccharides was characterized by HPGPC-ELSD, while the monosaccharide composition and total saccharides content were determined by HPLC-PDA and UV-VIS, respectively.
34118572	1	54	theme	Chang-Kang-Fang	175:189	arg1	CKF					200:202	CKF	200:202	CKF	200:202	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	1	54	theme	Chang-Kang-Fang	175:189	arg1	prescription					255:266	a multi-herbs traditional Chinese medicine (TCM) prescription	206:266	a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS)	206:310	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	1	54	theme	Chang-Kang-Fang	175:189	arg1	formula					191:197	Chang-Kang-Fang formula	175:197	Chang-Kang-Fang formula (CKF)	175:203	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	1	55	dep	cSHD	453:456	arg1	decoction					488:496	so called dispensing granule decoction	459:496	so called dispensing granule decoction	459:496	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	3	56	theme	small	803:807	arg1	molecules					809:817	Sixteen small molecules	795:817	Sixteen small molecules	795:817	Sixteen small molecules were simultaneously qualified and quantified by UPLC-QTOF-MS/MS, the molecular weight distribution of polysaccharides was characterized by HPGPC-ELSD, while the monosaccharide composition and total saccharides content were determined by HPLC-PDA and UV-VIS, respectively.
34118572	5	57	theme	significant	1526:1536	arg1	difference					1538:1547	a significant difference	1524:1547	a significant difference in the quality between MHD and cSHD	1524:1583	However, the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD were significantly lower than those in cSHD, whereas the content of polysaccharides in MHD was higher than that in cSHD, indicating that there is a significant difference in the quality between MHD and cSHD, in particular for the relative contents of major small molecules and polysaccharides.
34118572	3	58	dep	qualified	839:847	arg1	while					970:974	while	970:974	while	970:974	Sixteen small molecules were simultaneously qualified and quantified by UPLC-QTOF-MS/MS, the molecular weight distribution of polysaccharides was characterized by HPGPC-ELSD, while the monosaccharide composition and total saccharides content were determined by HPLC-PDA and UV-VIS, respectively.
34118572	2	59	theme	small	707:711	arg1	molecules					713:721	small molecules	707:721	small molecules	707:721	Herein, a new strategy by integrating multiple-chromatographic approaches to characterize both polysaccharides and small molecules was developed to compare the chemical consistency between MHD and cSHD.
34118572	5	60	theme	polysaccharides	1655:1669	arg1	contents					1617:1624	the relative contents	1604:1624	the relative contents of major small molecules and polysaccharides	1604:1669	However, the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD were significantly lower than those in cSHD, whereas the content of polysaccharides in MHD was higher than that in cSHD, indicating that there is a significant difference in the quality between MHD and cSHD, in particular for the relative contents of major small molecules and polysaccharides.
34118572	0	61	theme	preparations	100:111	arg1	consistency					69:79	chemical consistency	60:79	chemical consistency of Chang-Kang-Fang preparations from mixed-herb decoction and combined single-herb decoction	60:172	Integrating multiple-chromatographic approaches to evaluate chemical consistency of Chang-Kang-Fang preparations from mixed-herb decoction and combined single-herb decoction.
34118572	1	62	theme	combined	421:428	arg1	cSHD					453:456	cSHD	453:456	cSHD	453:456	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	1	62	theme	combined	421:428	arg1	decoction					442:450	combined single-herb decoction	421:450	combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades	421:517	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	1	63	theme	near	506:509	arg1	decades					511:517	the near decades	502:517	the near decades	502:517	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	4	64	theme	weight	1123:1128	arg1	range					1130:1134	molecular weight range	1113:1134	molecular weight range	1113:1134	It was found that the molecular weight range and monosaccharide composition of polysaccharides, as well as the composition of small molecules, were identical between MHD and cSHD.
34118572	0	65	theme	Chang-Kang-Fang	84:98	arg1	preparations					100:111	Chang-Kang-Fang preparations	84:111	Chang-Kang-Fang preparations from mixed-herb decoction and combined single-herb decoction	84:172	Integrating multiple-chromatographic approaches to evaluate chemical consistency of Chang-Kang-Fang preparations from mixed-herb decoction and combined single-herb decoction.
34118572	1	66	theme	traditional	348:358	arg1	form					360:363	the traditional form	344:363	the traditional form of mixed-herb decoction (MHD)	344:393	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	1	67	theme	irritable	281:289	arg1	syndrome					297:304	irritable bowel syndrome	281:304	irritable bowel syndrome (IBS)	281:310	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	1	67	theme	irritable	281:289	arg1	IBS					307:309	IBS	307:309	IBS	307:309	Chang-Kang-Fang formula (CKF), a multi-herbs traditional Chinese medicine (TCM) prescription for treating irritable bowel syndrome (IBS), has been clinically applied in the traditional form of mixed-herb decoction (MHD), or in the modern form of combined single-herb decoction (cSHD, so called dispensing granule decoction) in the near decades, but the chemical consistency between the MHD and cSHD is still unknown.
34118572	3	68	theme	total	1011:1015	arg1	content					1029:1035	total saccharides content	1011:1035	total saccharides content	1011:1035	Sixteen small molecules were simultaneously qualified and quantified by UPLC-QTOF-MS/MS, the molecular weight distribution of polysaccharides was characterized by HPGPC-ELSD, while the monosaccharide composition and total saccharides content were determined by HPLC-PDA and UV-VIS, respectively.
34118572	5	69	theme	major	1629:1633	arg1	molecules					1641:1649	major small molecules	1629:1649	major small molecules	1629:1649	However, the contents of berberine, epiberberine, coptisine, palmatine, albiflorin and paeoniflorin in MHD were significantly lower than those in cSHD, whereas the content of polysaccharides in MHD was higher than that in cSHD, indicating that there is a significant difference in the quality between MHD and cSHD, in particular for the relative contents of major small molecules and polysaccharides.
34118572	3	70	theme	weight	898:903	arg1	distribution					905:916	the molecular weight distribution	884:916	the molecular weight distribution of polysaccharides	884:935	Sixteen small molecules were simultaneously qualified and quantified by UPLC-QTOF-MS/MS, the molecular weight distribution of polysaccharides was characterized by HPGPC-ELSD, while the monosaccharide composition and total saccharides content were determined by HPLC-PDA and UV-VIS, respectively.
34118572	0	71	from	decoction	164:172	arg1	consistency					69:79	chemical consistency	60:79	chemical consistency of Chang-Kang-Fang preparations from mixed-herb decoction and combined single-herb decoction	60:172	Integrating multiple-chromatographic approaches to evaluate chemical consistency of Chang-Kang-Fang preparations from mixed-herb decoction and combined single-herb decoction.
34118572	0	71	from	decoction	164:172	arg1	preparations					100:111	Chang-Kang-Fang preparations	84:111	Chang-Kang-Fang preparations from mixed-herb decoction and combined single-herb decoction	84:172	Integrating multiple-chromatographic approaches to evaluate chemical consistency of Chang-Kang-Fang preparations from mixed-herb decoction and combined single-herb decoction.
33191778	8	0	theme	intragastric	1035:1046	arg1	gavage					1048:1053	a daily intragastric gavage	1027:1053	a daily intragastric gavage	1027:1053	For probiotic treatment, the mice received a daily intragastric gavage of 9.0 log10 cfu of EcN, beginning 10 days before colitis induction and continuing until the end of the experiment.
33191778	12	1	theme	EcN	1701:1703	arg1	treatment					1705:1713	EcN treatment	1701:1713	EcN treatment	1701:1713	Associated with mucosal preservation, restoration of secretory immunoglobulin A levels and reduction of interleukin (IL)-5, IL-13, tumour necrosis factor and interferon-γ levels were observed in EcN treatment.
33191778	14	2	theme	intestinal	2004:2013	arg1	microbiota					2015:2024	modulated intestinal microbiota	1994:2024	modulated intestinal microbiota	1994:2024	After faecal transplantation using GF mice, it was observed that EcN treatment in CV mice might result in modulated intestinal microbiota.
33191778	11	3	theme	probiotic	1438:1446	arg1	treatment					1448:1456	probiotic treatment	1438:1456	probiotic treatment	1438:1456	Reduction in bacterial translocation after probiotic treatment indicated protection of the intestinal barrier.
33191778	3	4	theme	Escherichia	487:497	arg1	EcN					517:519	EcN	517:519	EcN	517:519	Probiotics have been investigated as an adjuvant treatment for UC, and Escherichia coli Nissle 1917 (EcN) was the focus of our investigation.
33191778	3	4	theme	Escherichia	487:497	arg1	1917					511:514	Escherichia coli Nissle 1917	487:514	Escherichia coli Nissle 1917 (EcN)	487:520	Probiotics have been investigated as an adjuvant treatment for UC, and Escherichia coli Nissle 1917 (EcN) was the focus of our investigation.
33191778	3	4	theme	Escherichia	487:497	arg1	focus					530:534	the focus	526:534	the focus of our investigation	526:555	Probiotics have been investigated as an adjuvant treatment for UC, and Escherichia coli Nissle 1917 (EcN) was the focus of our investigation.
33191778	4	5	from	probiotic	632:640	arg1	model					661:665	an experimental model	645:665	an experimental model of chronic colitis in germ-free (GF) and conventional (CV) mice	645:729	The aim of this study was to investigate the preventive effect of the EcN probiotic in an experimental model of chronic colitis in germ-free (GF) and conventional (CV) mice.
33191778	6	6	theme	GF	815:816	arg1	mice					818:821	GF mice	815:821	GF mice	815:821	GF mice were used for a faecal microbiota transplantation assay.
33191778	10	7	theme	remission	1320:1328	arg1	periods					1330:1336	remission periods	1320:1336	remission periods	1320:1336	Daily Disease Activity Index (DAI) evolution demonstrated significant difference in remission periods after the first two DSS cycles and during the third one.
33191778	0	8	theme	Beneficial	0:9	arg1	effects					11:17	Beneficial effects	0:17	Beneficial effects	0:17	Beneficial effects resulting from oral administration of Escherichia coli Nissle 1917 on a chronic colitis model.
33191778	13	9	theme	colitis	1872:1878	arg1	groups					1880:1885	the control and colitis groups	1856:1885	groups	1880:1885	Finally, when microbiota modification was verified, 16S rRNA-based compositional analysis showed variation of intestinal microbiota between the control and colitis groups.
33191778	5	10	theme	permeability	789:800	arg1	experiments					802:812	permeability experiments	789:812	permeability experiments	789:812	CV female mice were used for clinical, immunological and permeability experiments.
33191778	12	11	theme	necrosis	1644:1651	arg1	factor					1653:1658	tumour necrosis factor	1637:1658	tumour necrosis factor	1637:1658	Associated with mucosal preservation, restoration of secretory immunoglobulin A levels and reduction of interleukin (IL)-5, IL-13, tumour necrosis factor and interferon-γ levels were observed in EcN treatment.
33191778	12	11	theme	necrosis	1644:1651	arg1	IL					1623:1624	interleukin (IL)-5	1610:1627	interleukin (IL)-5	1610:1627	Associated with mucosal preservation, restoration of secretory immunoglobulin A levels and reduction of interleukin (IL)-5, IL-13, tumour necrosis factor and interferon-γ levels were observed in EcN treatment.
33191778	1	12	theme	immune	194:199	arg1	response					201:208	a deregulated immune response	180:208	a deregulated immune response against intestinal microbiota in genetically susceptible individuals	180:277	Inflammatory bowel diseases (IBD) are chronic processes involving a deregulated immune response against intestinal microbiota in genetically susceptible individuals.
33191778	0	13	from	1917	81:84	arg1	model					107:111	a chronic colitis model	89:111	a chronic colitis model	89:111	Beneficial effects resulting from oral administration of Escherichia coli Nissle 1917 on a chronic colitis model.
33191778	7	14	theme	dextran	920:926	arg1	DSS					945:947	DSS	945:947	DSS	945:947	To induce colitis, three cycles of 3.0% dextran sulphate sodium (DSS) were administered to the animals.
33191778	7	14	theme	dextran	920:926	arg1	sodium					937:942	dextran sulphate sodium	920:942	3.0% dextran sulphate sodium (DSS)	915:948	To induce colitis, three cycles of 3.0% dextran sulphate sodium (DSS) were administered to the animals.
33191778	6	15	theme	transplantation	857:871	arg1	assay					873:877	a faecal microbiota transplantation assay	837:877	a faecal microbiota transplantation assay	837:877	GF mice were used for a faecal microbiota transplantation assay.
33191778	8	16	theme	experiment	1159:1168	arg1	end					1148:1150	the end	1144:1150	the end of the experiment	1144:1168	For probiotic treatment, the mice received a daily intragastric gavage of 9.0 log10 cfu of EcN, beginning 10 days before colitis induction and continuing until the end of the experiment.
33191778	3	17	theme	investigation	543:555	arg1	1917					511:514	Escherichia coli Nissle 1917	487:514	Escherichia coli Nissle 1917 (EcN)	487:520	Probiotics have been investigated as an adjuvant treatment for UC, and Escherichia coli Nissle 1917 (EcN) was the focus of our investigation.
33191778	3	17	theme	investigation	543:555	arg1	focus					530:534	the focus	526:534	the focus of our investigation	526:555	Probiotics have been investigated as an adjuvant treatment for UC, and Escherichia coli Nissle 1917 (EcN) was the focus of our investigation.
33191778	7	18	theme	3.0	915:917	arg1	%					918:918	%	918:918	%	918:918	To induce colitis, three cycles of 3.0% dextran sulphate sodium (DSS) were administered to the animals.
33191778	1	19	theme	Inflammatory	114:125	arg1	IBD					143:145	IBD	143:145	IBD	143:145	Inflammatory bowel diseases (IBD) are chronic processes involving a deregulated immune response against intestinal microbiota in genetically susceptible individuals.
33191778	1	19	theme	Inflammatory	114:125	arg1	diseases					133:140	Inflammatory bowel diseases	114:140	Inflammatory bowel diseases (IBD)	114:146	Inflammatory bowel diseases (IBD) are chronic processes involving a deregulated immune response against intestinal microbiota in genetically susceptible individuals.
33191778	1	19	theme	Inflammatory	114:125	arg1	processes					160:168	chronic processes	152:168	chronic processes involving a deregulated immune response against intestinal microbiota in genetically susceptible individuals	152:277	Inflammatory bowel diseases (IBD) are chronic processes involving a deregulated immune response against intestinal microbiota in genetically susceptible individuals.
33191778	6	20	theme	faecal	839:844	arg1	assay					873:877	a faecal microbiota transplantation assay	837:877	a faecal microbiota transplantation assay	837:877	GF mice were used for a faecal microbiota transplantation assay.
33191778	4	21	theme	chronic	670:676	arg1	colitis					678:684	chronic colitis	670:684	chronic colitis in germ-free (GF) and conventional (CV) mice	670:729	The aim of this study was to investigate the preventive effect of the EcN probiotic in an experimental model of chronic colitis in germ-free (GF) and conventional (CV) mice.
33191778	13	22	theme	microbiota	1837:1846	arg1	variation					1813:1821	variation	1813:1821	variation of intestinal microbiota between the control and colitis groups	1813:1885	Finally, when microbiota modification was verified, 16S rRNA-based compositional analysis showed variation of intestinal microbiota between the control and colitis groups.
33191778	1	23	from	microbiota	229:238	arg1	individuals					267:277	genetically susceptible individuals	243:277	genetically susceptible individuals	243:277	Inflammatory bowel diseases (IBD) are chronic processes involving a deregulated immune response against intestinal microbiota in genetically susceptible individuals.
33191778	4	24	from	mice	726:729	arg1	model					661:665	an experimental model	645:665	an experimental model of chronic colitis in germ-free (GF) and conventional (CV) mice	645:729	The aim of this study was to investigate the preventive effect of the EcN probiotic in an experimental model of chronic colitis in germ-free (GF) and conventional (CV) mice.
33191778	4	25	theme	probiotic	632:640	arg1	EcN					628:630	the EcN	624:630	the EcN probiotic in an experimental model of chronic colitis in germ-free (GF) and conventional (CV) mice	624:729	The aim of this study was to investigate the preventive effect of the EcN probiotic in an experimental model of chronic colitis in germ-free (GF) and conventional (CV) mice.
33191778	15	26	theme	treated	2113:2119	arg1	group					2121:2125	treated group	2113:2125	treated group	2113:2125	This was observed indirectly in the reduced daily DAI, when colitis was compared with treated group.
33191778	12	27	theme	interleukin	1610:1620	arg1	IL					1623:1624	interleukin (IL)-5	1610:1627	interleukin (IL)-5	1610:1627	Associated with mucosal preservation, restoration of secretory immunoglobulin A levels and reduction of interleukin (IL)-5, IL-13, tumour necrosis factor and interferon-γ levels were observed in EcN treatment.
33191778	12	27	theme	interleukin	1610:1620	arg1	factor					1653:1658	tumour necrosis factor	1637:1658	tumour necrosis factor	1637:1658	Associated with mucosal preservation, restoration of secretory immunoglobulin A levels and reduction of interleukin (IL)-5, IL-13, tumour necrosis factor and interferon-γ levels were observed in EcN treatment.
33191778	12	27	theme	interleukin	1610:1620	arg1	IL-13					1630:1634	IL-13	1630:1634	IL-13	1630:1634	Associated with mucosal preservation, restoration of secretory immunoglobulin A levels and reduction of interleukin (IL)-5, IL-13, tumour necrosis factor and interferon-γ levels were observed in EcN treatment.
33191778	10	28	theme	first	1348:1352	arg1	cycles					1362:1367	the first two DSS cycles	1344:1367	the first two DSS cycles	1344:1367	Daily Disease Activity Index (DAI) evolution demonstrated significant difference in remission periods after the first two DSS cycles and during the third one.
33191778	11	29	theme	bacterial	1408:1416	arg1	translocation					1418:1430	bacterial translocation	1408:1430	bacterial translocation	1408:1430	Reduction in bacterial translocation after probiotic treatment indicated protection of the intestinal barrier.
33191778	0	30	theme	1917	81:84	arg1	administration					39:52	oral administration	34:52	oral administration of Escherichia coli Nissle 1917 on a chronic colitis model	34:111	Beneficial effects resulting from oral administration of Escherichia coli Nissle 1917 on a chronic colitis model.
33191778	12	31	theme	IL	1623:1624	arg1	restoration					1544:1554	restoration	1544:1554	restoration of secretory immunoglobulin A levels	1544:1591	Associated with mucosal preservation, restoration of secretory immunoglobulin A levels and reduction of interleukin (IL)-5, IL-13, tumour necrosis factor and interferon-γ levels were observed in EcN treatment.
33191778	12	31	theme	IL	1623:1624	arg1	preservation					1530:1541	mucosal preservation	1522:1541	mucosal preservation	1522:1541	Associated with mucosal preservation, restoration of secretory immunoglobulin A levels and reduction of interleukin (IL)-5, IL-13, tumour necrosis factor and interferon-γ levels were observed in EcN treatment.
33191778	12	31	theme	IL	1623:1624	arg1	reduction					1597:1605	reduction	1597:1605	reduction of interleukin (IL)-5, IL-13, tumour necrosis factor	1597:1658	Associated with mucosal preservation, restoration of secretory immunoglobulin A levels and reduction of interleukin (IL)-5, IL-13, tumour necrosis factor and interferon-γ levels were observed in EcN treatment.
33191778	15	32	located	observed	2036:2043	arg2	This					2027:2030	This	2027:2030	This	2027:2030	This was observed indirectly in the reduced daily DAI, when colitis was compared with treated group.
33191778	15	32	located	observed	2036:2043	arg1	DAI					2077:2079	DAI	2077:2079	DAI	2077:2079	This was observed indirectly in the reduced daily DAI, when colitis was compared with treated group.
33191778	6	33	used	used	828:831	arg2	mice					818:821	GF mice	815:821	GF mice	815:821	GF mice were used for a faecal microbiota transplantation assay.
33191778	11	34	from	Reduction	1395:1403	arg1	translocation					1418:1430	bacterial translocation	1408:1430	bacterial translocation	1408:1430	Reduction in bacterial translocation after probiotic treatment indicated protection of the intestinal barrier.
33191778	12	35	theme	levels	1586:1591	arg1	restoration					1544:1554	restoration	1544:1554	restoration of secretory immunoglobulin A levels	1544:1591	Associated with mucosal preservation, restoration of secretory immunoglobulin A levels and reduction of interleukin (IL)-5, IL-13, tumour necrosis factor and interferon-γ levels were observed in EcN treatment.
33191778	12	35	theme	levels	1586:1591	arg1	preservation					1530:1541	mucosal preservation	1522:1541	mucosal preservation	1522:1541	Associated with mucosal preservation, restoration of secretory immunoglobulin A levels and reduction of interleukin (IL)-5, IL-13, tumour necrosis factor and interferon-γ levels were observed in EcN treatment.
33191778	12	35	theme	levels	1586:1591	arg1	reduction					1597:1605	reduction	1597:1605	reduction of interleukin (IL)-5, IL-13, tumour necrosis factor	1597:1658	Associated with mucosal preservation, restoration of secretory immunoglobulin A levels and reduction of interleukin (IL)-5, IL-13, tumour necrosis factor and interferon-γ levels were observed in EcN treatment.
33191778	4	36	theme	conventional	708:719	arg1	mice					726:729	germ-free (GF) and conventional (CV) mice	689:729	mice	726:729	The aim of this study was to investigate the preventive effect of the EcN probiotic in an experimental model of chronic colitis in germ-free (GF) and conventional (CV) mice.
33191778	13	37	theme	16S	1768:1770	arg1	analysis					1797:1804	16S rRNA-based compositional analysis	1768:1804	16S rRNA-based compositional analysis	1768:1804	Finally, when microbiota modification was verified, 16S rRNA-based compositional analysis showed variation of intestinal microbiota between the control and colitis groups.
33191778	2	38	theme	colorectal	391:400	arg1	CRC					410:412	CRC	410:412	CRC	410:412	Ulcerative colitis (UC) is an IBD restricted to colonic mucosa and its chronicity is a predisposing factor for colorectal cancer (CRC).
33191778	2	38	theme	colorectal	391:400	arg1	cancer					402:407	colorectal cancer	391:407	colorectal cancer (CRC)	391:413	Ulcerative colitis (UC) is an IBD restricted to colonic mucosa and its chronicity is a predisposing factor for colorectal cancer (CRC).
33191778	0	39	theme	colitis	99:105	arg1	model					107:111	a chronic colitis model	89:111	a chronic colitis model	89:111	Beneficial effects resulting from oral administration of Escherichia coli Nissle 1917 on a chronic colitis model.
33191778	10	40	theme	DSS	1358:1360	arg1	cycles					1362:1367	the first two DSS cycles	1344:1367	the first two DSS cycles	1344:1367	Daily Disease Activity Index (DAI) evolution demonstrated significant difference in remission periods after the first two DSS cycles and during the third one.
33191778	13	41	theme	compositional	1783:1795	arg1	analysis					1797:1804	16S rRNA-based compositional analysis	1768:1804	16S rRNA-based compositional analysis	1768:1804	Finally, when microbiota modification was verified, 16S rRNA-based compositional analysis showed variation of intestinal microbiota between the control and colitis groups.
33191778	5	42	theme	CV	732:733	arg1	mice					742:745	CV female mice	732:745	CV female mice	732:745	CV female mice were used for clinical, immunological and permeability experiments.
33191778	3	43	dep	Escherichia	487:497	arg1	coli					499:502	coli	499:502	coli	499:502	Probiotics have been investigated as an adjuvant treatment for UC, and Escherichia coli Nissle 1917 (EcN) was the focus of our investigation.
33191778	12	44	theme	immunoglobulin	1569:1582	arg1	levels					1586:1591	secretory immunoglobulin A levels	1559:1591	secretory immunoglobulin A levels	1559:1591	Associated with mucosal preservation, restoration of secretory immunoglobulin A levels and reduction of interleukin (IL)-5, IL-13, tumour necrosis factor and interferon-γ levels were observed in EcN treatment.
33191778	0	45	theme	oral	34:37	arg1	administration					39:52	oral administration	34:52	oral administration of Escherichia coli Nissle 1917 on a chronic colitis model	34:111	Beneficial effects resulting from oral administration of Escherichia coli Nissle 1917 on a chronic colitis model.
33191778	14	46	theme	faecal	1894:1899	arg1	transplantation					1901:1915	faecal transplantation	1894:1915	faecal transplantation using GF mice	1894:1929	After faecal transplantation using GF mice, it was observed that EcN treatment in CV mice might result in modulated intestinal microbiota.
33191778	14	47	theme	CV	1970:1971	arg1	mice					1973:1976	CV mice	1970:1976	CV mice	1970:1976	After faecal transplantation using GF mice, it was observed that EcN treatment in CV mice might result in modulated intestinal microbiota.
33191778	14	48	from	treatment	1957:1965	arg1	mice					1973:1976	CV mice	1970:1976	CV mice	1970:1976	After faecal transplantation using GF mice, it was observed that EcN treatment in CV mice might result in modulated intestinal microbiota.
33191778	13	49	theme	control	1860:1866	arg1	groups					1880:1885	the control and colitis groups	1856:1885	groups	1880:1885	Finally, when microbiota modification was verified, 16S rRNA-based compositional analysis showed variation of intestinal microbiota between the control and colitis groups.
33191778	4	50	theme	CV	722:723	arg1	mice					726:729	germ-free (GF) and conventional (CV) mice	689:729	mice	726:729	The aim of this study was to investigate the preventive effect of the EcN probiotic in an experimental model of chronic colitis in germ-free (GF) and conventional (CV) mice.
33191778	11	51	theme	barrier	1497:1503	arg1	protection					1468:1477	protection	1468:1477	protection of the intestinal barrier	1468:1503	Reduction in bacterial translocation after probiotic treatment indicated protection of the intestinal barrier.
33191778	2	52	theme	colonic	328:334	arg1	mucosa					336:341	colonic mucosa	328:341	colonic mucosa	328:341	Ulcerative colitis (UC) is an IBD restricted to colonic mucosa and its chronicity is a predisposing factor for colorectal cancer (CRC).
33191778	1	53	theme	intestinal	218:227	arg1	microbiota					229:238	intestinal microbiota	218:238	intestinal microbiota in genetically susceptible individuals	218:277	Inflammatory bowel diseases (IBD) are chronic processes involving a deregulated immune response against intestinal microbiota in genetically susceptible individuals.
33191778	10	54	theme	Index	1259:1263	arg1	evolution					1271:1279	Daily Disease Activity Index (DAI) evolution	1236:1279	Daily Disease Activity Index (DAI) evolution	1236:1279	Daily Disease Activity Index (DAI) evolution demonstrated significant difference in remission periods after the first two DSS cycles and during the third one.
33191778	8	55	theme	colitis	1105:1111	arg1	induction					1113:1121	colitis induction	1105:1121	colitis induction	1105:1121	For probiotic treatment, the mice received a daily intragastric gavage of 9.0 log10 cfu of EcN, beginning 10 days before colitis induction and continuing until the end of the experiment.
33191778	10	56	theme	Disease	1242:1248	arg1	DAI					1266:1268	DAI	1266:1268	DAI	1266:1268	Daily Disease Activity Index (DAI) evolution demonstrated significant difference in remission periods after the first two DSS cycles and during the third one.
33191778	10	56	theme	Disease	1242:1248	arg1	Index					1259:1263	Daily Disease Activity Index	1236:1263	Daily Disease Activity Index (DAI) evolution	1236:1279	Daily Disease Activity Index (DAI) evolution demonstrated significant difference in remission periods after the first two DSS cycles and during the third one.
33191778	1	57	theme	susceptible	255:265	arg1	individuals					267:277	genetically susceptible individuals	243:277	genetically susceptible individuals	243:277	Inflammatory bowel diseases (IBD) are chronic processes involving a deregulated immune response against intestinal microbiota in genetically susceptible individuals.
33191778	8	58	theme	daily	1029:1033	arg1	gavage					1048:1053	a daily intragastric gavage	1027:1053	a daily intragastric gavage	1027:1053	For probiotic treatment, the mice received a daily intragastric gavage of 9.0 log10 cfu of EcN, beginning 10 days before colitis induction and continuing until the end of the experiment.
33191778	12	59	theme	interferon-γ	1664:1675	arg1	levels					1677:1682	interferon-γ levels	1664:1682	interferon-γ levels	1664:1682	Associated with mucosal preservation, restoration of secretory immunoglobulin A levels and reduction of interleukin (IL)-5, IL-13, tumour necrosis factor and interferon-γ levels were observed in EcN treatment.
33191778	5	60	used	used	752:755	arg2	mice					742:745	CV female mice	732:745	CV female mice	732:745	CV female mice were used for clinical, immunological and permeability experiments.
33191778	1	61	theme	chronic	152:158	arg1	diseases					133:140	Inflammatory bowel diseases	114:140	Inflammatory bowel diseases (IBD)	114:146	Inflammatory bowel diseases (IBD) are chronic processes involving a deregulated immune response against intestinal microbiota in genetically susceptible individuals.
33191778	1	61	theme	chronic	152:158	arg1	processes					160:168	chronic processes	152:168	chronic processes involving a deregulated immune response against intestinal microbiota in genetically susceptible individuals	152:277	Inflammatory bowel diseases (IBD) are chronic processes involving a deregulated immune response against intestinal microbiota in genetically susceptible individuals.
33191778	12	62	theme	tumour	1637:1642	arg1	factor					1653:1658	tumour necrosis factor	1637:1658	tumour necrosis factor	1637:1658	Associated with mucosal preservation, restoration of secretory immunoglobulin A levels and reduction of interleukin (IL)-5, IL-13, tumour necrosis factor and interferon-γ levels were observed in EcN treatment.
33191778	12	62	theme	tumour	1637:1642	arg1	IL					1623:1624	interleukin (IL)-5	1610:1627	interleukin (IL)-5	1610:1627	Associated with mucosal preservation, restoration of secretory immunoglobulin A levels and reduction of interleukin (IL)-5, IL-13, tumour necrosis factor and interferon-γ levels were observed in EcN treatment.
33191778	4	63	theme	preventive	603:612	arg1	effect					614:619	the preventive effect	599:619	the preventive effect of the EcN probiotic in an experimental model of chronic colitis in germ-free (GF) and conventional (CV) mice	599:729	The aim of this study was to investigate the preventive effect of the EcN probiotic in an experimental model of chronic colitis in germ-free (GF) and conventional (CV) mice.
33191778	2	64	theme	restricted	314:323	arg1	colitis					291:297	Ulcerative colitis	280:297	Ulcerative colitis (UC)	280:302	Ulcerative colitis (UC) is an IBD restricted to colonic mucosa and its chronicity is a predisposing factor for colorectal cancer (CRC).
33191778	2	64	theme	restricted	314:323	arg1	IBD					310:312	an IBD	307:312	an IBD restricted to colonic mucosa	307:341	Ulcerative colitis (UC) is an IBD restricted to colonic mucosa and its chronicity is a predisposing factor for colorectal cancer (CRC).
33191778	8	65	theme	cfu	1068:1070	arg1	gavage					1048:1053	a daily intragastric gavage	1027:1053	a daily intragastric gavage	1027:1053	For probiotic treatment, the mice received a daily intragastric gavage of 9.0 log10 cfu of EcN, beginning 10 days before colitis induction and continuing until the end of the experiment.
33191778	12	66	theme	mucosal	1522:1528	arg1	preservation					1530:1541	mucosal preservation	1522:1541	mucosal preservation	1522:1541	Associated with mucosal preservation, restoration of secretory immunoglobulin A levels and reduction of interleukin (IL)-5, IL-13, tumour necrosis factor and interferon-γ levels were observed in EcN treatment.
33191778	10	67	theme	significant	1294:1304	arg1	difference					1306:1315	significant difference	1294:1315	significant difference in remission periods	1294:1336	Daily Disease Activity Index (DAI) evolution demonstrated significant difference in remission periods after the first two DSS cycles and during the third one.
33191778	1	68	theme	deregulated	182:192	arg1	response					201:208	a deregulated immune response	180:208	a deregulated immune response against intestinal microbiota in genetically susceptible individuals	180:277	Inflammatory bowel diseases (IBD) are chronic processes involving a deregulated immune response against intestinal microbiota in genetically susceptible individuals.
33191778	8	69	theme	EcN	1075:1077	arg1	cfu					1068:1070	9.0 log10 cfu	1058:1070	9.0 log10 cfu of EcN	1058:1077	For probiotic treatment, the mice received a daily intragastric gavage of 9.0 log10 cfu of EcN, beginning 10 days before colitis induction and continuing until the end of the experiment.
33191778	16	70	attach	presented	2147:2155	arg1	model					2184:2188	this model	2179:2188	this model	2179:2188	In conclusion, EcN presented beneficial effects in this model, suggesting its usefulness for treating UC.
33191778	16	70	attach	presented	2147:2155	arg2	EcN					2143:2145	EcN	2143:2145	EcN	2143:2145	In conclusion, EcN presented beneficial effects in this model, suggesting its usefulness for treating UC.
33191778	16	70	attach	presented	2147:2155	arg1	conclusion					2131:2140	conclusion	2131:2140	conclusion	2131:2140	In conclusion, EcN presented beneficial effects in this model, suggesting its usefulness for treating UC.
33191778	7	71	theme	sulphate	928:935	arg1	DSS					945:947	DSS	945:947	DSS	945:947	To induce colitis, three cycles of 3.0% dextran sulphate sodium (DSS) were administered to the animals.
33191778	7	71	theme	sulphate	928:935	arg1	sodium					937:942	dextran sulphate sodium	920:942	3.0% dextran sulphate sodium (DSS)	915:948	To induce colitis, three cycles of 3.0% dextran sulphate sodium (DSS) were administered to the animals.
33191778	6	72	theme	microbiota	846:855	arg1	assay					873:877	a faecal microbiota transplantation assay	837:877	a faecal microbiota transplantation assay	837:877	GF mice were used for a faecal microbiota transplantation assay.
33191778	0	73	from	model	107:111	arg1	administration					39:52	oral administration	34:52	oral administration of Escherichia coli Nissle 1917 on a chronic colitis model	34:111	Beneficial effects resulting from oral administration of Escherichia coli Nissle 1917 on a chronic colitis model.
33191778	4	74	theme	experimental	648:659	arg1	model					661:665	an experimental model	645:665	an experimental model of chronic colitis in germ-free (GF) and conventional (CV) mice	645:729	The aim of this study was to investigate the preventive effect of the EcN probiotic in an experimental model of chronic colitis in germ-free (GF) and conventional (CV) mice.
33191778	13	75	theme	intestinal	1826:1835	arg1	microbiota					1837:1846	intestinal microbiota	1826:1846	intestinal microbiota	1826:1846	Finally, when microbiota modification was verified, 16S rRNA-based compositional analysis showed variation of intestinal microbiota between the control and colitis groups.
33191778	7	76	theme	sodium	937:942	arg1	cycles					905:910	three cycles	899:910	three cycles of 3.0% dextran sulphate sodium (DSS)	899:948	To induce colitis, three cycles of 3.0% dextran sulphate sodium (DSS) were administered to the animals.
33191778	2	77	theme	Ulcerative	280:289	arg1	UC					300:301	UC	300:301	UC	300:301	Ulcerative colitis (UC) is an IBD restricted to colonic mucosa and its chronicity is a predisposing factor for colorectal cancer (CRC).
33191778	2	77	theme	Ulcerative	280:289	arg1	colitis					291:297	Ulcerative colitis	280:297	Ulcerative colitis (UC)	280:302	Ulcerative colitis (UC) is an IBD restricted to colonic mucosa and its chronicity is a predisposing factor for colorectal cancer (CRC).
33191778	2	77	theme	Ulcerative	280:289	arg1	IBD					310:312	an IBD	307:312	an IBD restricted to colonic mucosa	307:341	Ulcerative colitis (UC) is an IBD restricted to colonic mucosa and its chronicity is a predisposing factor for colorectal cancer (CRC).
33191778	7	78	theme	%	918:918	arg1	DSS					945:947	DSS	945:947	DSS	945:947	To induce colitis, three cycles of 3.0% dextran sulphate sodium (DSS) were administered to the animals.
33191778	7	78	theme	%	918:918	arg1	sodium					937:942	dextran sulphate sodium	920:942	3.0% dextran sulphate sodium (DSS)	915:948	To induce colitis, three cycles of 3.0% dextran sulphate sodium (DSS) were administered to the animals.
33191778	1	79	theme	bowel	127:131	arg1	IBD					143:145	IBD	143:145	IBD	143:145	Inflammatory bowel diseases (IBD) are chronic processes involving a deregulated immune response against intestinal microbiota in genetically susceptible individuals.
33191778	1	79	theme	bowel	127:131	arg1	diseases					133:140	Inflammatory bowel diseases	114:140	Inflammatory bowel diseases (IBD)	114:146	Inflammatory bowel diseases (IBD) are chronic processes involving a deregulated immune response against intestinal microbiota in genetically susceptible individuals.
33191778	1	79	theme	bowel	127:131	arg1	processes					160:168	chronic processes	152:168	chronic processes involving a deregulated immune response against intestinal microbiota in genetically susceptible individuals	152:277	Inflammatory bowel diseases (IBD) are chronic processes involving a deregulated immune response against intestinal microbiota in genetically susceptible individuals.
33191778	0	80	theme	Nissle	74:79	arg1	1917					81:84	Escherichia coli Nissle 1917	57:84	Escherichia coli Nissle 1917 on a chronic colitis model	57:111	Beneficial effects resulting from oral administration of Escherichia coli Nissle 1917 on a chronic colitis model.
33191778	4	81	theme	EcN	628:630	arg1	effect					614:619	the preventive effect	599:619	the preventive effect of the EcN probiotic in an experimental model of chronic colitis in germ-free (GF) and conventional (CV) mice	599:729	The aim of this study was to investigate the preventive effect of the EcN probiotic in an experimental model of chronic colitis in germ-free (GF) and conventional (CV) mice.
33191778	0	82	theme	chronic	91:97	arg1	model					107:111	a chronic colitis model	89:111	a chronic colitis model	89:111	Beneficial effects resulting from oral administration of Escherichia coli Nissle 1917 on a chronic colitis model.
33191778	8	83	dep	gavage	1048:1053	arg1	continuing					1127:1136	continuing	1127:1136	continuing until the end of the experiment	1127:1168	For probiotic treatment, the mice received a daily intragastric gavage of 9.0 log10 cfu of EcN, beginning 10 days before colitis induction and continuing until the end of the experiment.
33191778	8	83	dep	gavage	1048:1053	arg1	beginning					1080:1088	beginning	1080:1088	beginning 10 days before colitis induction	1080:1121	For probiotic treatment, the mice received a daily intragastric gavage of 9.0 log10 cfu of EcN, beginning 10 days before colitis induction and continuing until the end of the experiment.
33191778	9	84	theme	beneficial	1185:1194	arg1	effects					1196:1202	beneficial effects	1185:1202	beneficial effects	1185:1202	EcN presented beneficial effects when administered preventively.
33191778	4	85	from	GF	700:701	arg1	model					661:665	an experimental model	645:665	an experimental model of chronic colitis in germ-free (GF) and conventional (CV) mice	645:729	The aim of this study was to investigate the preventive effect of the EcN probiotic in an experimental model of chronic colitis in germ-free (GF) and conventional (CV) mice.
33191778	12	86	theme	A	1584:1584	arg1	levels					1586:1591	secretory immunoglobulin A levels	1559:1591	secretory immunoglobulin A levels	1559:1591	Associated with mucosal preservation, restoration of secretory immunoglobulin A levels and reduction of interleukin (IL)-5, IL-13, tumour necrosis factor and interferon-γ levels were observed in EcN treatment.
33191778	0	87	from	administration	39:52	arg1	model					107:111	a chronic colitis model	89:111	a chronic colitis model	89:111	Beneficial effects resulting from oral administration of Escherichia coli Nissle 1917 on a chronic colitis model.
33191778	13	88	theme	rRNA-based	1772:1781	arg1	analysis					1797:1804	16S rRNA-based compositional analysis	1768:1804	16S rRNA-based compositional analysis	1768:1804	Finally, when microbiota modification was verified, 16S rRNA-based compositional analysis showed variation of intestinal microbiota between the control and colitis groups.
33191778	14	89	theme	modulated	1994:2002	arg1	microbiota					2015:2024	modulated intestinal microbiota	1994:2024	modulated intestinal microbiota	1994:2024	After faecal transplantation using GF mice, it was observed that EcN treatment in CV mice might result in modulated intestinal microbiota.
33191778	4	90	theme	colitis	678:684	arg1	model					661:665	an experimental model	645:665	an experimental model of chronic colitis in germ-free (GF) and conventional (CV) mice	645:729	The aim of this study was to investigate the preventive effect of the EcN probiotic in an experimental model of chronic colitis in germ-free (GF) and conventional (CV) mice.
33191778	13	91	theme	microbiota	1730:1739	arg1	modification					1741:1752	microbiota modification	1730:1752	microbiota modification	1730:1752	Finally, when microbiota modification was verified, 16S rRNA-based compositional analysis showed variation of intestinal microbiota between the control and colitis groups.
33191778	2	92	theme	predisposing	367:378	arg1	chronicity					351:360	its chronicity	347:360	its chronicity	347:360	Ulcerative colitis (UC) is an IBD restricted to colonic mucosa and its chronicity is a predisposing factor for colorectal cancer (CRC).
33191778	2	92	theme	predisposing	367:378	arg1	factor					380:385	a predisposing factor	365:385	a predisposing factor for colorectal cancer (CRC)	365:413	Ulcerative colitis (UC) is an IBD restricted to colonic mucosa and its chronicity is a predisposing factor for colorectal cancer (CRC).
33191778	5	93	theme	female	735:740	arg1	mice					742:745	CV female mice	732:745	CV female mice	732:745	CV female mice were used for clinical, immunological and permeability experiments.
33191778	4	94	from	colitis	678:684	arg1	mice					726:729	germ-free (GF) and conventional (CV) mice	689:729	mice	726:729	The aim of this study was to investigate the preventive effect of the EcN probiotic in an experimental model of chronic colitis in germ-free (GF) and conventional (CV) mice.
33191778	4	94	from	colitis	678:684	arg1	GF					700:701	germ-free (GF) and conventional (CV) mice	689:729	GF	700:701	The aim of this study was to investigate the preventive effect of the EcN probiotic in an experimental model of chronic colitis in germ-free (GF) and conventional (CV) mice.
33191778	14	95	theme	GF	1923:1924	arg1	mice					1926:1929	GF mice	1923:1929	GF mice	1923:1929	After faecal transplantation using GF mice, it was observed that EcN treatment in CV mice might result in modulated intestinal microbiota.
33191778	10	96	theme	third	1384:1388	arg1	one					1390:1392	the third one	1380:1392	the third one	1380:1392	Daily Disease Activity Index (DAI) evolution demonstrated significant difference in remission periods after the first two DSS cycles and during the third one.
33191778	4	97	theme	germ-free	689:697	arg1	GF					700:701	germ-free (GF) and conventional (CV) mice	689:729	GF	700:701	The aim of this study was to investigate the preventive effect of the EcN probiotic in an experimental model of chronic colitis in germ-free (GF) and conventional (CV) mice.
33191778	3	98	theme	adjuvant	456:463	arg1	treatment					465:473	an adjuvant treatment	453:473	an adjuvant treatment for UC	453:480	Probiotics have been investigated as an adjuvant treatment for UC, and Escherichia coli Nissle 1917 (EcN) was the focus of our investigation.
33191778	3	98	theme	adjuvant	456:463	arg1	Probiotics					416:425	Probiotics	416:425	Probiotics	416:425	Probiotics have been investigated as an adjuvant treatment for UC, and Escherichia coli Nissle 1917 (EcN) was the focus of our investigation.
33191778	8	99	theme	probiotic	988:996	arg1	treatment					998:1006	probiotic treatment	988:1006	probiotic treatment	988:1006	For probiotic treatment, the mice received a daily intragastric gavage of 9.0 log10 cfu of EcN, beginning 10 days before colitis induction and continuing until the end of the experiment.
33191778	0	100	dep	Escherichia	57:67	arg1	coli					69:72	coli	69:72	coli	69:72	Beneficial effects resulting from oral administration of Escherichia coli Nissle 1917 on a chronic colitis model.
33191778	12	101	theme	secretory	1559:1567	arg1	levels					1586:1591	secretory immunoglobulin A levels	1559:1591	secretory immunoglobulin A levels	1559:1591	Associated with mucosal preservation, restoration of secretory immunoglobulin A levels and reduction of interleukin (IL)-5, IL-13, tumour necrosis factor and interferon-γ levels were observed in EcN treatment.
33191778	12	102	located	observed	1689:1696	arg2	levels					1677:1682	interferon-γ levels	1664:1682	interferon-γ levels	1664:1682	Associated with mucosal preservation, restoration of secretory immunoglobulin A levels and reduction of interleukin (IL)-5, IL-13, tumour necrosis factor and interferon-γ levels were observed in EcN treatment.
33191778	12	102	located	observed	1689:1696	arg1	treatment					1705:1713	EcN treatment	1701:1713	EcN treatment	1701:1713	Associated with mucosal preservation, restoration of secretory immunoglobulin A levels and reduction of interleukin (IL)-5, IL-13, tumour necrosis factor and interferon-γ levels were observed in EcN treatment.
33191778	10	103	theme	Daily	1236:1240	arg1	DAI					1266:1268	DAI	1266:1268	DAI	1266:1268	Daily Disease Activity Index (DAI) evolution demonstrated significant difference in remission periods after the first two DSS cycles and during the third one.
33191778	10	103	theme	Daily	1236:1240	arg1	Index					1259:1263	Daily Disease Activity Index	1236:1263	Daily Disease Activity Index (DAI) evolution	1236:1279	Daily Disease Activity Index (DAI) evolution demonstrated significant difference in remission periods after the first two DSS cycles and during the third one.
33191778	0	104	theme	Escherichia	57:67	arg1	1917					81:84	Escherichia coli Nissle 1917	57:84	Escherichia coli Nissle 1917 on a chronic colitis model	57:111	Beneficial effects resulting from oral administration of Escherichia coli Nissle 1917 on a chronic colitis model.
33191778	11	105	theme	intestinal	1486:1495	arg1	barrier					1497:1503	the intestinal barrier	1482:1503	the intestinal barrier	1482:1503	Reduction in bacterial translocation after probiotic treatment indicated protection of the intestinal barrier.
33191778	14	106	theme	EcN	1953:1955	arg1	treatment					1957:1965	EcN treatment	1953:1965	EcN treatment in CV mice	1953:1976	After faecal transplantation using GF mice, it was observed that EcN treatment in CV mice might result in modulated intestinal microbiota.
33191778	4	107	from	model	661:665	arg1	mice					726:729	germ-free (GF) and conventional (CV) mice	689:729	mice	726:729	The aim of this study was to investigate the preventive effect of the EcN probiotic in an experimental model of chronic colitis in germ-free (GF) and conventional (CV) mice.
33191778	4	107	from	model	661:665	arg1	probiotic					632:640	probiotic	632:640	probiotic	632:640	The aim of this study was to investigate the preventive effect of the EcN probiotic in an experimental model of chronic colitis in germ-free (GF) and conventional (CV) mice.
33191778	4	107	from	model	661:665	arg1	GF					700:701	germ-free (GF) and conventional (CV) mice	689:729	GF	700:701	The aim of this study was to investigate the preventive effect of the EcN probiotic in an experimental model of chronic colitis in germ-free (GF) and conventional (CV) mice.
33191778	16	108	theme	beneficial	2157:2166	arg1	effects					2168:2174	beneficial effects	2157:2174	beneficial effects	2157:2174	In conclusion, EcN presented beneficial effects in this model, suggesting its usefulness for treating UC.
33191778	10	109	from	difference	1306:1315	arg1	periods					1330:1336	remission periods	1320:1336	remission periods	1320:1336	Daily Disease Activity Index (DAI) evolution demonstrated significant difference in remission periods after the first two DSS cycles and during the third one.
33191778	4	110	theme	study	574:578	arg1	aim					562:564	The aim	558:564	The aim of this study	558:578	The aim of this study was to investigate the preventive effect of the EcN probiotic in an experimental model of chronic colitis in germ-free (GF) and conventional (CV) mice.
33191778	10	111	theme	Activity	1250:1257	arg1	DAI					1266:1268	DAI	1266:1268	DAI	1266:1268	Daily Disease Activity Index (DAI) evolution demonstrated significant difference in remission periods after the first two DSS cycles and during the third one.
33191778	10	111	theme	Activity	1250:1257	arg1	Index					1259:1263	Daily Disease Activity Index	1236:1263	Daily Disease Activity Index (DAI) evolution	1236:1279	Daily Disease Activity Index (DAI) evolution demonstrated significant difference in remission periods after the first two DSS cycles and during the third one.
33191778	3	112	theme	Nissle	504:509	arg1	EcN					517:519	EcN	517:519	EcN	517:519	Probiotics have been investigated as an adjuvant treatment for UC, and Escherichia coli Nissle 1917 (EcN) was the focus of our investigation.
33191778	3	112	theme	Nissle	504:509	arg1	1917					511:514	Escherichia coli Nissle 1917	487:514	Escherichia coli Nissle 1917 (EcN)	487:520	Probiotics have been investigated as an adjuvant treatment for UC, and Escherichia coli Nissle 1917 (EcN) was the focus of our investigation.
33191778	3	112	theme	Nissle	504:509	arg1	focus					530:534	the focus	526:534	the focus of our investigation	526:555	Probiotics have been investigated as an adjuvant treatment for UC, and Escherichia coli Nissle 1917 (EcN) was the focus of our investigation.
34646541	7	0	theme	polysaccharides	1031:1045	arg1	SCFAs					986:990	the total SCFAs	976:990	the total SCFAs of cooked, uncooked quinoa, and quinoa polysaccharides	976:1045	After 24 hr anaerobic incubation, the total SCFAs of cooked, uncooked quinoa, and quinoa polysaccharides were 82.99, 77.11, and 82.73 mM, respectively with a pH decrease.
34646541	7	0	theme	polysaccharides	1031:1045	arg1	82.99					1052:1056	82.99	1052:1056	82.99	1052:1056	After 24 hr anaerobic incubation, the total SCFAs of cooked, uncooked quinoa, and quinoa polysaccharides were 82.99, 77.11, and 82.73 mM, respectively with a pH decrease.
34646541	9	1	theme	Quinoa	1283:1288	arg1	polysaccharides					1290:1304	Quinoa polysaccharides	1283:1304	Quinoa polysaccharides	1283:1304	Quinoa polysaccharides can be considered prebiotic due to their ability to increase Bifidobacterium and Collinsella.
34646541	10	2	from	effect	1479:1484	arg1	microbiota					1499:1508	the fecal microbiota	1489:1508	the fecal microbiota which represents different distribution	1489:1548	Principal component analysis (PCA) showed that there was a distinct modulating effect on the fecal microbiota which represents different distribution.
34646541	7	3	theme	quinoa	1012:1017	arg1	SCFAs					986:990	the total SCFAs	976:990	the total SCFAs of cooked, uncooked quinoa, and quinoa polysaccharides	976:1045	After 24 hr anaerobic incubation, the total SCFAs of cooked, uncooked quinoa, and quinoa polysaccharides were 82.99, 77.11, and 82.73 mM, respectively with a pH decrease.
34646541	7	3	theme	quinoa	1012:1017	arg1	82.99					1052:1056	82.99	1052:1056	82.99	1052:1056	After 24 hr anaerobic incubation, the total SCFAs of cooked, uncooked quinoa, and quinoa polysaccharides were 82.99, 77.11, and 82.73 mM, respectively with a pH decrease.
34646541	11	4	theme	composition	1703:1713	arg1	production					1743:1752	microbiota composition and short-chain fatty acids production	1692:1752	microbiota composition and short-chain fatty acids production	1692:1752	Our research suggests that quinoa and quinoa polysaccharides have a prebiotic potential due to their association with the positive shifts in microbiota composition and short-chain fatty acids production, which highlights the importance of further studies around this topic.
34646541	8	5	theme	class	1139:1143	arg1	level					1145:1149	the phylum, genus, and class level	1116:1149	level	1145:1149	At the phylum, genus, and class level, it has been found that the quinoa substrates enhance the growth of certain beneficial bacteria such as Prevotella and Bacteroides.
34646541	10	6	theme	distinct	1459:1466	arg1	effect					1479:1484	a distinct modulating effect	1457:1484	a distinct modulating effect on the fecal microbiota which represents different distribution	1457:1548	Principal component analysis (PCA) showed that there was a distinct modulating effect on the fecal microbiota which represents different distribution.
34646541	5	7	theme	cooked	658:663	arg1	quinoa					678:683	the cooked and uncooked quinoa	654:683	the cooked and uncooked quinoa	654:683	The digestibility of the cooked and uncooked quinoa after simulation of human digestion was 69.04% and 64.09%, respectively.
34646541	4	8	theme	polysaccharides	594:608	arg1	extraction					610:619	polysaccharides extraction	594:619	polysaccharides extraction	594:619	The yield of polysaccharides extraction was 15.45%.
34646541	6	9	from	effect	762:767	arg1	products					819:826	their metabolic products	803:826	their metabolic products	803:826	The effect on the microbiota composition and their metabolic products was determined by the assessment of pH, short-chain fatty acids (SCFAs), and changes in the bacterial population.
34646541	6	9	from	effect	762:767	arg1	composition					787:797	the microbiota composition	772:797	the microbiota composition	772:797	The effect on the microbiota composition and their metabolic products was determined by the assessment of pH, short-chain fatty acids (SCFAs), and changes in the bacterial population.
34646541	11	10	theme	acids	1737:1741	arg1	production					1743:1752	microbiota composition and short-chain fatty acids production	1692:1752	microbiota composition and short-chain fatty acids production	1692:1752	Our research suggests that quinoa and quinoa polysaccharides have a prebiotic potential due to their association with the positive shifts in microbiota composition and short-chain fatty acids production, which highlights the importance of further studies around this topic.
34646541	5	11	theme	uncooked	669:676	arg1	quinoa					678:683	the cooked and uncooked quinoa	654:683	the cooked and uncooked quinoa	654:683	The digestibility of the cooked and uncooked quinoa after simulation of human digestion was 69.04% and 64.09%, respectively.
34646541	6	12	theme	fatty	880:884	arg1	SCFAs					893:897	SCFAs	893:897	SCFAs	893:897	The effect on the microbiota composition and their metabolic products was determined by the assessment of pH, short-chain fatty acids (SCFAs), and changes in the bacterial population.
34646541	6	12	theme	fatty	880:884	arg1	acids					886:890	short-chain fatty acids	868:890	short-chain fatty acids (SCFAs)	868:898	The effect on the microbiota composition and their metabolic products was determined by the assessment of pH, short-chain fatty acids (SCFAs), and changes in the bacterial population.
34646541	11	13	theme	short-chain	1719:1729	arg1	acids					1737:1741	short-chain fatty acids	1719:1741	short-chain fatty acids	1719:1741	Our research suggests that quinoa and quinoa polysaccharides have a prebiotic potential due to their association with the positive shifts in microbiota composition and short-chain fatty acids production, which highlights the importance of further studies around this topic.
34646541	1	14	from	intestine	211:219	arg1	important					149:157	important	149:157	important	149:157	It has been shown that whole grains and dietary fiber are important for their fermentation characteristics in the large intestine, drawing more and more attention to quinoa and quinoa polysaccharides.
34646541	0	15	from	microbiota	79:88	arg1	study					9:13	In vitro study	0:13	In vitro study of the effect of quinoa and quinoa polysaccharides on human gut microbiota	0:88	In vitro study of the effect of quinoa and quinoa polysaccharides on human gut microbiota.
34646541	7	16	theme	anaerobic	954:962	arg1	incubation					964:973	24 hr anaerobic incubation	948:973	24 hr anaerobic incubation	948:973	After 24 hr anaerobic incubation, the total SCFAs of cooked, uncooked quinoa, and quinoa polysaccharides were 82.99, 77.11, and 82.73 mM, respectively with a pH decrease.
34646541	11	17	theme	due	1639:1641	arg1	potential					1629:1637	a prebiotic potential	1617:1637	a prebiotic potential due to their association with the positive shifts in microbiota composition and short-chain fatty acids production, which highlights the importance of further studies around this topic	1617:1822	Our research suggests that quinoa and quinoa polysaccharides have a prebiotic potential due to their association with the positive shifts in microbiota composition and short-chain fatty acids production, which highlights the importance of further studies around this topic.
34646541	5	18	theme	digestion	711:719	arg1	simulation					691:700	simulation	691:700	simulation of human digestion	691:719	The digestibility of the cooked and uncooked quinoa after simulation of human digestion was 69.04% and 64.09%, respectively.
34646541	3	19	theme	fecal	563:567	arg1	microbiota					569:578	human fecal microbiota	557:578	human fecal microbiota	557:578	The modulatory effect of the quinoa and quinoa polysaccharides (QPs) on the gut microbiota was evaluated by the in vitro fermentation using human fecal microbiota.
34646541	1	20	theme	whole	114:118	arg1	grains					120:125	whole grains	114:125	whole grains	114:125	It has been shown that whole grains and dietary fiber are important for their fermentation characteristics in the large intestine, drawing more and more attention to quinoa and quinoa polysaccharides.
34646541	6	21	theme	bacterial	920:928	arg1	population					930:939	the bacterial population	916:939	the bacterial population	916:939	The effect on the microbiota composition and their metabolic products was determined by the assessment of pH, short-chain fatty acids (SCFAs), and changes in the bacterial population.
34646541	0	22	from	study	9:13	arg1	microbiota					79:88	human gut microbiota	69:88	human gut microbiota	69:88	In vitro study of the effect of quinoa and quinoa polysaccharides on human gut microbiota.
34646541	6	23	from	population	930:939	arg1	assessment					850:859	the assessment	846:859	the assessment of pH, short-chain fatty acids (SCFAs), and changes in the bacterial population	846:939	The effect on the microbiota composition and their metabolic products was determined by the assessment of pH, short-chain fatty acids (SCFAs), and changes in the bacterial population.
34646541	11	24	theme	positive	1673:1680	arg1	shifts					1682:1687	the positive shifts	1669:1687	the positive shifts in microbiota composition and short-chain fatty acids production, which highlights the importance of further studies around this topic	1669:1822	Our research suggests that quinoa and quinoa polysaccharides have a prebiotic potential due to their association with the positive shifts in microbiota composition and short-chain fatty acids production, which highlights the importance of further studies around this topic.
34646541	0	25	theme	polysaccharides	50:64	arg1	effect					22:27	the effect	18:27	the effect of quinoa and quinoa polysaccharides on human gut microbiota	18:88	In vitro study of the effect of quinoa and quinoa polysaccharides on human gut microbiota.
34646541	0	26	theme	human	69:73	arg1	microbiota					79:88	human gut microbiota	69:88	human gut microbiota	69:88	In vitro study of the effect of quinoa and quinoa polysaccharides on human gut microbiota.
34646541	3	27	dep	quinoa	446:451	arg1	the					442:444	the	442:444	the	442:444	The modulatory effect of the quinoa and quinoa polysaccharides (QPs) on the gut microbiota was evaluated by the in vitro fermentation using human fecal microbiota.
34646541	3	27	dep	quinoa	446:451	arg1	polysaccharides					464:478	polysaccharides	464:478	polysaccharides	464:478	The modulatory effect of the quinoa and quinoa polysaccharides (QPs) on the gut microbiota was evaluated by the in vitro fermentation using human fecal microbiota.
34646541	6	28	from	changes	905:911	arg1	population					930:939	the bacterial population	916:939	the bacterial population	916:939	The effect on the microbiota composition and their metabolic products was determined by the assessment of pH, short-chain fatty acids (SCFAs), and changes in the bacterial population.
34646541	2	29	theme	seeds	351:355	arg1	effect					334:339	the prebiotic effect	320:339	the prebiotic effect of quinoa seeds and quinoa polysaccharides	320:382	In this study, we evaluated the prebiotic effect of quinoa seeds and quinoa polysaccharides after human simulated digestion.
34646541	0	30	theme	quinoa	43:48	arg1	polysaccharides					50:64	quinoa and quinoa polysaccharides	32:64	quinoa and quinoa polysaccharides	32:64	In vitro study of the effect of quinoa and quinoa polysaccharides on human gut microbiota.
34646541	8	31	theme	beneficial	1227:1236	arg1	Bacteroides					1270:1280	Bacteroides	1270:1280	Bacteroides	1270:1280	At the phylum, genus, and class level, it has been found that the quinoa substrates enhance the growth of certain beneficial bacteria such as Prevotella and Bacteroides.
34646541	8	31	theme	beneficial	1227:1236	arg1	bacteria					1238:1245	certain beneficial bacteria	1219:1245	certain beneficial bacteria such as Prevotella and Bacteroides	1219:1280	At the phylum, genus, and class level, it has been found that the quinoa substrates enhance the growth of certain beneficial bacteria such as Prevotella and Bacteroides.
34646541	8	31	theme	beneficial	1227:1236	arg1	Prevotella					1255:1264	Prevotella	1255:1264	Prevotella	1255:1264	At the phylum, genus, and class level, it has been found that the quinoa substrates enhance the growth of certain beneficial bacteria such as Prevotella and Bacteroides.
34646541	10	32	theme	different	1527:1535	arg1	distribution					1537:1548	different distribution	1527:1548	different distribution	1527:1548	Principal component analysis (PCA) showed that there was a distinct modulating effect on the fecal microbiota which represents different distribution.
34646541	0	33	theme	In	0:1	arg1	study					9:13	In vitro study	0:13	In vitro study of the effect of quinoa and quinoa polysaccharides on human gut microbiota	0:88	In vitro study of the effect of quinoa and quinoa polysaccharides on human gut microbiota.
34646541	1	34	theme	more	239:242	arg1	attention					244:252	more and more attention	230:252	more and more attention	230:252	It has been shown that whole grains and dietary fiber are important for their fermentation characteristics in the large intestine, drawing more and more attention to quinoa and quinoa polysaccharides.
34646541	2	35	theme	simulated	396:404	arg1	digestion					406:414	human simulated digestion	390:414	human simulated digestion	390:414	In this study, we evaluated the prebiotic effect of quinoa seeds and quinoa polysaccharides after human simulated digestion.
34646541	6	36	theme	metabolic	809:817	arg1	products					819:826	their metabolic products	803:826	their metabolic products	803:826	The effect on the microbiota composition and their metabolic products was determined by the assessment of pH, short-chain fatty acids (SCFAs), and changes in the bacterial population.
34646541	2	37	theme	quinoa	361:366	arg1	polysaccharides					368:382	quinoa polysaccharides	361:382	quinoa polysaccharides	361:382	In this study, we evaluated the prebiotic effect of quinoa seeds and quinoa polysaccharides after human simulated digestion.
34646541	3	38	dep	in	529:530	arg1	vitro					532:536	vitro	532:536	vitro	532:536	The modulatory effect of the quinoa and quinoa polysaccharides (QPs) on the gut microbiota was evaluated by the in vitro fermentation using human fecal microbiota.
34646541	8	39	located	found	1164:1168	arg1	genus					1128:1132	the phylum, genus, and class level	1116:1149	genus	1128:1132	At the phylum, genus, and class level, it has been found that the quinoa substrates enhance the growth of certain beneficial bacteria such as Prevotella and Bacteroides.
34646541	8	39	located	found	1164:1168	arg2	it					1152:1153	it	1152:1153	it	1152:1153	At the phylum, genus, and class level, it has been found that the quinoa substrates enhance the growth of certain beneficial bacteria such as Prevotella and Bacteroides.
34646541	8	39	located	found	1164:1168	arg1	level					1145:1149	the phylum, genus, and class level	1116:1149	level	1145:1149	At the phylum, genus, and class level, it has been found that the quinoa substrates enhance the growth of certain beneficial bacteria such as Prevotella and Bacteroides.
34646541	6	40	theme	microbiota	776:785	arg1	composition					787:797	the microbiota composition	772:797	the microbiota composition	772:797	The effect on the microbiota composition and their metabolic products was determined by the assessment of pH, short-chain fatty acids (SCFAs), and changes in the bacterial population.
34646541	10	41	theme	Principal	1400:1408	arg1	PCA					1430:1432	PCA	1430:1432	PCA	1430:1432	Principal component analysis (PCA) showed that there was a distinct modulating effect on the fecal microbiota which represents different distribution.
34646541	10	41	theme	Principal	1400:1408	arg1	analysis					1420:1427	Principal component analysis	1400:1427	Principal component analysis (PCA)	1400:1433	Principal component analysis (PCA) showed that there was a distinct modulating effect on the fecal microbiota which represents different distribution.
34646541	6	42	from	assessment	850:859	arg1	population					930:939	the bacterial population	916:939	the bacterial population	916:939	The effect on the microbiota composition and their metabolic products was determined by the assessment of pH, short-chain fatty acids (SCFAs), and changes in the bacterial population.
34646541	2	43	theme	prebiotic	324:332	arg1	effect					334:339	the prebiotic effect	320:339	the prebiotic effect of quinoa seeds and quinoa polysaccharides	320:382	In this study, we evaluated the prebiotic effect of quinoa seeds and quinoa polysaccharides after human simulated digestion.
34646541	4	44	theme	extraction	610:619	arg1	yield					585:589	The yield	581:589	The yield of polysaccharides extraction	581:619	The yield of polysaccharides extraction was 15.45%.
34646541	4	44	theme	extraction	610:619	arg1	%					630:630	15.45%	625:630	15.45%	625:630	The yield of polysaccharides extraction was 15.45%.
34646541	1	45	theme	large	205:209	arg1	intestine					211:219	the large intestine	201:219	the large intestine	201:219	It has been shown that whole grains and dietary fiber are important for their fermentation characteristics in the large intestine, drawing more and more attention to quinoa and quinoa polysaccharides.
34646541	6	46	theme	pH	864:865	arg1	assessment					850:859	the assessment	846:859	the assessment of pH, short-chain fatty acids (SCFAs), and changes in the bacterial population	846:939	The effect on the microbiota composition and their metabolic products was determined by the assessment of pH, short-chain fatty acids (SCFAs), and changes in the bacterial population.
34646541	0	47	dep	In	0:1	arg1	vitro					3:7	vitro	3:7	vitro	3:7	In vitro study of the effect of quinoa and quinoa polysaccharides on human gut microbiota.
34646541	6	48	from	pH	864:865	arg1	population					930:939	the bacterial population	916:939	the bacterial population	916:939	The effect on the microbiota composition and their metabolic products was determined by the assessment of pH, short-chain fatty acids (SCFAs), and changes in the bacterial population.
34646541	7	49	theme	quinoa	1024:1029	arg1	polysaccharides					1031:1045	quinoa polysaccharides	1024:1045	quinoa polysaccharides	1024:1045	After 24 hr anaerobic incubation, the total SCFAs of cooked, uncooked quinoa, and quinoa polysaccharides were 82.99, 77.11, and 82.73 mM, respectively with a pH decrease.
34646541	1	50	dep	quinoa	257:262	arg1	polysaccharides					275:289	polysaccharides	275:289	polysaccharides	275:289	It has been shown that whole grains and dietary fiber are important for their fermentation characteristics in the large intestine, drawing more and more attention to quinoa and quinoa polysaccharides.
34646541	3	51	theme	gut	493:495	arg1	microbiota					497:506	the gut microbiota	489:506	the gut microbiota	489:506	The modulatory effect of the quinoa and quinoa polysaccharides (QPs) on the gut microbiota was evaluated by the in vitro fermentation using human fecal microbiota.
34646541	1	52	theme	more	230:233	arg1	attention					244:252	more and more attention	230:252	more and more attention	230:252	It has been shown that whole grains and dietary fiber are important for their fermentation characteristics in the large intestine, drawing more and more attention to quinoa and quinoa polysaccharides.
34646541	7	53	theme	uncooked	1003:1010	arg1	quinoa					1012:1017	uncooked quinoa	1003:1017	uncooked quinoa	1003:1017	After 24 hr anaerobic incubation, the total SCFAs of cooked, uncooked quinoa, and quinoa polysaccharides were 82.99, 77.11, and 82.73 mM, respectively with a pH decrease.
34646541	1	54	theme	dietary	131:137	arg1	fiber					139:143	dietary fiber	131:143	dietary fiber	131:143	It has been shown that whole grains and dietary fiber are important for their fermentation characteristics in the large intestine, drawing more and more attention to quinoa and quinoa polysaccharides.
34646541	8	55	theme	phylum	1120:1125	arg1	genus					1128:1132	the phylum, genus, and class level	1116:1149	genus	1128:1132	At the phylum, genus, and class level, it has been found that the quinoa substrates enhance the growth of certain beneficial bacteria such as Prevotella and Bacteroides.
34646541	7	56	theme	cooked	995:1000	arg1	SCFAs					986:990	the total SCFAs	976:990	the total SCFAs of cooked, uncooked quinoa, and quinoa polysaccharides	976:1045	After 24 hr anaerobic incubation, the total SCFAs of cooked, uncooked quinoa, and quinoa polysaccharides were 82.99, 77.11, and 82.73 mM, respectively with a pH decrease.
34646541	7	56	theme	cooked	995:1000	arg1	82.99					1052:1056	82.99	1052:1056	82.99	1052:1056	After 24 hr anaerobic incubation, the total SCFAs of cooked, uncooked quinoa, and quinoa polysaccharides were 82.99, 77.11, and 82.73 mM, respectively with a pH decrease.
34646541	10	57	theme	modulating	1468:1477	arg1	effect					1479:1484	a distinct modulating effect	1457:1484	a distinct modulating effect on the fecal microbiota which represents different distribution	1457:1548	Principal component analysis (PCA) showed that there was a distinct modulating effect on the fecal microbiota which represents different distribution.
34646541	11	58	theme	microbiota	1692:1701	arg1	composition					1703:1713	microbiota composition	1692:1713	microbiota composition	1692:1713	Our research suggests that quinoa and quinoa polysaccharides have a prebiotic potential due to their association with the positive shifts in microbiota composition and short-chain fatty acids production, which highlights the importance of further studies around this topic.
34646541	1	59	theme	fermentation	169:180	arg1	characteristics					182:196	their fermentation characteristics	163:196	their fermentation characteristics	163:196	It has been shown that whole grains and dietary fiber are important for their fermentation characteristics in the large intestine, drawing more and more attention to quinoa and quinoa polysaccharides.
34646541	6	60	theme	acids	886:890	arg1	assessment					850:859	the assessment	846:859	the assessment of pH, short-chain fatty acids (SCFAs), and changes in the bacterial population	846:939	The effect on the microbiota composition and their metabolic products was determined by the assessment of pH, short-chain fatty acids (SCFAs), and changes in the bacterial population.
34646541	11	61	from	shifts	1682:1687	arg1	production					1743:1752	microbiota composition and short-chain fatty acids production	1692:1752	microbiota composition and short-chain fatty acids production	1692:1752	Our research suggests that quinoa and quinoa polysaccharides have a prebiotic potential due to their association with the positive shifts in microbiota composition and short-chain fatty acids production, which highlights the importance of further studies around this topic.
34646541	10	62	theme	fecal	1493:1497	arg1	microbiota					1499:1508	the fecal microbiota	1489:1508	the fecal microbiota which represents different distribution	1489:1548	Principal component analysis (PCA) showed that there was a distinct modulating effect on the fecal microbiota which represents different distribution.
34646541	5	63	theme	quinoa	678:683	arg1	digestibility					637:649	The digestibility	633:649	The digestibility of the cooked and uncooked quinoa after simulation of human digestion	633:719	The digestibility of the cooked and uncooked quinoa after simulation of human digestion was 69.04% and 64.09%, respectively.
34646541	5	63	theme	quinoa	678:683	arg1	%					730:730	69.04%	725:730	69.04%	725:730	The digestibility of the cooked and uncooked quinoa after simulation of human digestion was 69.04% and 64.09%, respectively.
34646541	3	64	theme	in	529:530	arg1	fermentation					538:549	the in vitro fermentation	525:549	the in vitro fermentation using human fecal microbiota	525:578	The modulatory effect of the quinoa and quinoa polysaccharides (QPs) on the gut microbiota was evaluated by the in vitro fermentation using human fecal microbiota.
34646541	6	65	from	acids	886:890	arg1	population					930:939	the bacterial population	916:939	the bacterial population	916:939	The effect on the microbiota composition and their metabolic products was determined by the assessment of pH, short-chain fatty acids (SCFAs), and changes in the bacterial population.
34646541	6	66	theme	short-chain	868:878	arg1	SCFAs					893:897	SCFAs	893:897	SCFAs	893:897	The effect on the microbiota composition and their metabolic products was determined by the assessment of pH, short-chain fatty acids (SCFAs), and changes in the bacterial population.
34646541	6	66	theme	short-chain	868:878	arg1	acids					886:890	short-chain fatty acids	868:890	short-chain fatty acids (SCFAs)	868:898	The effect on the microbiota composition and their metabolic products was determined by the assessment of pH, short-chain fatty acids (SCFAs), and changes in the bacterial population.
34646541	11	67	theme	fatty	1731:1735	arg1	acids					1737:1741	short-chain fatty acids	1719:1741	short-chain fatty acids	1719:1741	Our research suggests that quinoa and quinoa polysaccharides have a prebiotic potential due to their association with the positive shifts in microbiota composition and short-chain fatty acids production, which highlights the importance of further studies around this topic.
34646541	7	68	theme	24 hr	948:952	arg1	incubation					964:973	24 hr anaerobic incubation	948:973	24 hr anaerobic incubation	948:973	After 24 hr anaerobic incubation, the total SCFAs of cooked, uncooked quinoa, and quinoa polysaccharides were 82.99, 77.11, and 82.73 mM, respectively with a pH decrease.
34646541	11	69	contain	have	1612:1615	arg2	potential					1629:1637	a prebiotic potential	1617:1637	a prebiotic potential due to their association with the positive shifts in microbiota composition and short-chain fatty acids production, which highlights the importance of further studies around this topic	1617:1822	Our research suggests that quinoa and quinoa polysaccharides have a prebiotic potential due to their association with the positive shifts in microbiota composition and short-chain fatty acids production, which highlights the importance of further studies around this topic.
34646541	11	69	contain	have	1612:1615	arg1	polysaccharides					1596:1610	quinoa and quinoa polysaccharides	1578:1610	quinoa and quinoa polysaccharides	1578:1610	Our research suggests that quinoa and quinoa polysaccharides have a prebiotic potential due to their association with the positive shifts in microbiota composition and short-chain fatty acids production, which highlights the importance of further studies around this topic.
34646541	5	70	theme	human	705:709	arg1	digestion					711:719	human digestion	705:719	human digestion	705:719	The digestibility of the cooked and uncooked quinoa after simulation of human digestion was 69.04% and 64.09%, respectively.
34646541	3	71	theme	human	557:561	arg1	microbiota					569:578	human fecal microbiota	557:578	human fecal microbiota	557:578	The modulatory effect of the quinoa and quinoa polysaccharides (QPs) on the gut microbiota was evaluated by the in vitro fermentation using human fecal microbiota.
34646541	1	72	from	important	149:157	arg1	intestine					211:219	the large intestine	201:219	the large intestine	201:219	It has been shown that whole grains and dietary fiber are important for their fermentation characteristics in the large intestine, drawing more and more attention to quinoa and quinoa polysaccharides.
34646541	7	73	theme	pH	1100:1101	arg1	decrease					1103:1110	a pH decrease	1098:1110	a pH decrease	1098:1110	After 24 hr anaerobic incubation, the total SCFAs of cooked, uncooked quinoa, and quinoa polysaccharides were 82.99, 77.11, and 82.73 mM, respectively with a pH decrease.
34646541	11	74	with	association	1652:1662	arg1	shifts					1682:1687	the positive shifts	1669:1687	the positive shifts in microbiota composition and short-chain fatty acids production, which highlights the importance of further studies around this topic	1669:1822	Our research suggests that quinoa and quinoa polysaccharides have a prebiotic potential due to their association with the positive shifts in microbiota composition and short-chain fatty acids production, which highlights the importance of further studies around this topic.
34646541	8	75	theme	quinoa	1179:1184	arg1	substrates					1186:1195	the quinoa substrates	1175:1195	the quinoa substrates	1175:1195	At the phylum, genus, and class level, it has been found that the quinoa substrates enhance the growth of certain beneficial bacteria such as Prevotella and Bacteroides.
34646541	0	76	theme	gut	75:77	arg1	microbiota					79:88	human gut microbiota	69:88	human gut microbiota	69:88	In vitro study of the effect of quinoa and quinoa polysaccharides on human gut microbiota.
34646541	6	77	theme	changes	905:911	arg1	assessment					850:859	the assessment	846:859	the assessment of pH, short-chain fatty acids (SCFAs), and changes in the bacterial population	846:939	The effect on the microbiota composition and their metabolic products was determined by the assessment of pH, short-chain fatty acids (SCFAs), and changes in the bacterial population.
34646541	0	78	from	effect	22:27	arg1	microbiota					79:88	human gut microbiota	69:88	human gut microbiota	69:88	In vitro study of the effect of quinoa and quinoa polysaccharides on human gut microbiota.
34646541	2	79	theme	quinoa	344:349	arg1	seeds					351:355	quinoa seeds	344:355	quinoa seeds	344:355	In this study, we evaluated the prebiotic effect of quinoa seeds and quinoa polysaccharides after human simulated digestion.
34646541	11	80	theme	further	1790:1796	arg1	studies					1798:1804	further studies	1790:1804	further studies around this topic	1790:1822	Our research suggests that quinoa and quinoa polysaccharides have a prebiotic potential due to their association with the positive shifts in microbiota composition and short-chain fatty acids production, which highlights the importance of further studies around this topic.
34646541	8	81	theme	certain	1219:1225	arg1	Bacteroides					1270:1280	Bacteroides	1270:1280	Bacteroides	1270:1280	At the phylum, genus, and class level, it has been found that the quinoa substrates enhance the growth of certain beneficial bacteria such as Prevotella and Bacteroides.
34646541	8	81	theme	certain	1219:1225	arg1	bacteria					1238:1245	certain beneficial bacteria	1219:1245	certain beneficial bacteria such as Prevotella and Bacteroides	1219:1280	At the phylum, genus, and class level, it has been found that the quinoa substrates enhance the growth of certain beneficial bacteria such as Prevotella and Bacteroides.
34646541	8	81	theme	certain	1219:1225	arg1	Prevotella					1255:1264	Prevotella	1255:1264	Prevotella	1255:1264	At the phylum, genus, and class level, it has been found that the quinoa substrates enhance the growth of certain beneficial bacteria such as Prevotella and Bacteroides.
34646541	2	82	theme	human	390:394	arg1	digestion					406:414	human simulated digestion	390:414	human simulated digestion	390:414	In this study, we evaluated the prebiotic effect of quinoa seeds and quinoa polysaccharides after human simulated digestion.
34646541	11	83	theme	quinoa	1589:1594	arg1	polysaccharides					1596:1610	quinoa and quinoa polysaccharides	1578:1610	quinoa and quinoa polysaccharides	1578:1610	Our research suggests that quinoa and quinoa polysaccharides have a prebiotic potential due to their association with the positive shifts in microbiota composition and short-chain fatty acids production, which highlights the importance of further studies around this topic.
34646541	8	84	theme	bacteria	1238:1245	arg1	growth					1209:1214	the growth	1205:1214	the growth of certain beneficial bacteria such as Prevotella and Bacteroides	1205:1280	At the phylum, genus, and class level, it has been found that the quinoa substrates enhance the growth of certain beneficial bacteria such as Prevotella and Bacteroides.
34646541	2	85	theme	polysaccharides	368:382	arg1	effect					334:339	the prebiotic effect	320:339	the prebiotic effect of quinoa seeds and quinoa polysaccharides	320:382	In this study, we evaluated the prebiotic effect of quinoa seeds and quinoa polysaccharides after human simulated digestion.
34646541	0	86	theme	effect	22:27	arg1	study					9:13	In vitro study	0:13	In vitro study of the effect of quinoa and quinoa polysaccharides on human gut microbiota	0:88	In vitro study of the effect of quinoa and quinoa polysaccharides on human gut microbiota.
34646541	11	87	theme	quinoa	1578:1583	arg1	polysaccharides					1596:1610	quinoa and quinoa polysaccharides	1578:1610	quinoa and quinoa polysaccharides	1578:1610	Our research suggests that quinoa and quinoa polysaccharides have a prebiotic potential due to their association with the positive shifts in microbiota composition and short-chain fatty acids production, which highlights the importance of further studies around this topic.
34646541	3	88	theme	modulatory	421:430	arg1	effect					432:437	The modulatory effect	417:437	The modulatory effect of the quinoa and quinoa polysaccharides (QPs) on the gut microbiota	417:506	The modulatory effect of the quinoa and quinoa polysaccharides (QPs) on the gut microbiota was evaluated by the in vitro fermentation using human fecal microbiota.
34646541	0	89	theme	quinoa	32:37	arg1	polysaccharides					50:64	quinoa and quinoa polysaccharides	32:64	quinoa and quinoa polysaccharides	32:64	In vitro study of the effect of quinoa and quinoa polysaccharides on human gut microbiota.
34646541	7	90	theme	total	980:984	arg1	SCFAs					986:990	the total SCFAs	976:990	the total SCFAs of cooked, uncooked quinoa, and quinoa polysaccharides	976:1045	After 24 hr anaerobic incubation, the total SCFAs of cooked, uncooked quinoa, and quinoa polysaccharides were 82.99, 77.11, and 82.73 mM, respectively with a pH decrease.
34646541	7	90	theme	total	980:984	arg1	82.99					1052:1056	82.99	1052:1056	82.99	1052:1056	After 24 hr anaerobic incubation, the total SCFAs of cooked, uncooked quinoa, and quinoa polysaccharides were 82.99, 77.11, and 82.73 mM, respectively with a pH decrease.
34646541	10	91	theme	component	1410:1418	arg1	PCA					1430:1432	PCA	1430:1432	PCA	1430:1432	Principal component analysis (PCA) showed that there was a distinct modulating effect on the fecal microbiota which represents different distribution.
34646541	10	91	theme	component	1410:1418	arg1	analysis					1420:1427	Principal component analysis	1400:1427	Principal component analysis (PCA)	1400:1433	Principal component analysis (PCA) showed that there was a distinct modulating effect on the fecal microbiota which represents different distribution.
34646541	11	92	theme	prebiotic	1619:1627	arg1	potential					1629:1637	a prebiotic potential	1617:1637	a prebiotic potential due to their association with the positive shifts in microbiota composition and short-chain fatty acids production, which highlights the importance of further studies around this topic	1617:1822	Our research suggests that quinoa and quinoa polysaccharides have a prebiotic potential due to their association with the positive shifts in microbiota composition and short-chain fatty acids production, which highlights the importance of further studies around this topic.
34646541	3	93	from	effect	432:437	arg1	microbiota					497:506	the gut microbiota	489:506	the gut microbiota	489:506	The modulatory effect of the quinoa and quinoa polysaccharides (QPs) on the gut microbiota was evaluated by the in vitro fermentation using human fecal microbiota.
34646541	3	94	theme	quinoa	457:462	arg1	effect					432:437	The modulatory effect	417:437	The modulatory effect of the quinoa and quinoa polysaccharides (QPs) on the gut microbiota	417:506	The modulatory effect of the quinoa and quinoa polysaccharides (QPs) on the gut microbiota was evaluated by the in vitro fermentation using human fecal microbiota.
34646541	3	95	theme	quinoa	446:451	arg1	effect					432:437	The modulatory effect	417:437	The modulatory effect of the quinoa and quinoa polysaccharides (QPs) on the gut microbiota	417:506	The modulatory effect of the quinoa and quinoa polysaccharides (QPs) on the gut microbiota was evaluated by the in vitro fermentation using human fecal microbiota.
34646541	11	96	theme	studies	1798:1804	arg1	importance					1776:1785	the importance	1772:1785	the importance of further studies around this topic	1772:1822	Our research suggests that quinoa and quinoa polysaccharides have a prebiotic potential due to their association with the positive shifts in microbiota composition and short-chain fatty acids production, which highlights the importance of further studies around this topic.
32519940	12	0	theme	similarity	1360:1369	arg1	results					1295:1301	The results	1291:1301	The results of phenotypic and chemotaxonomic, 16S rRNA gene sequence similarity, and whole genome analyses	1291:1396	The results of phenotypic and chemotaxonomic, 16S rRNA gene sequence similarity, and whole genome analyses support strain BCM23-1T as representing a novel species of Terrilactibacillus for which the name Terrilactibacillus tamarindi sp.
32519940	7	1	theme	aminophospholipid	682:698	arg1	presence					608:615	the presence	604:615	the presence of diphosphatidylglycerol, phosphatidylglycerol, an unidentified aminophospholipid, an unidentified phospholipid and an unidentified lipid	604:754	Polar lipids analysis revealed the presence of diphosphatidylglycerol, phosphatidylglycerol, an unidentified aminophospholipid, an unidentified phospholipid and an unidentified lipid.
32519940	9	2	theme	16S	887:889	arg1	%					1000:1000	98.3 %	995:1000	98.3 %	995:1000	The 16S rRNA gene sequence similarity between strain BCM23-1T and Terrilactibacillus laevilacticus NK26-11T was 98.3 %.
32519940	9	2	theme	16S	887:889	arg1	similarity					910:919	The 16S rRNA gene sequence similarity	883:919	The 16S rRNA gene sequence similarity between strain BCM23-1T and Terrilactibacillus laevilacticus NK26-11T	883:989	The 16S rRNA gene sequence similarity between strain BCM23-1T and Terrilactibacillus laevilacticus NK26-11T was 98.3 %.
32519940	10	3	theme	%	1133:1133	arg1	content					1114:1120	an in silico DNA G+C content	1093:1120	an in silico DNA G+C content of 37.1 mol%	1093:1133	The draft genome of BCM23-1T was 3.24 Mb in size and contained 3088 coding sequences with an in silico DNA G+C content of 37.1 mol%.
32519940	12	4	theme	16S	1337:1339	arg1	similarity					1360:1369	16S rRNA gene sequence similarity	1337:1369	16S rRNA gene sequence similarity	1337:1369	The results of phenotypic and chemotaxonomic, 16S rRNA gene sequence similarity, and whole genome analyses support strain BCM23-1T as representing a novel species of Terrilactibacillus for which the name Terrilactibacillus tamarindi sp.
32519940	9	5	theme	gene	896:899	arg1	%					1000:1000	98.3 %	995:1000	98.3 %	995:1000	The 16S rRNA gene sequence similarity between strain BCM23-1T and Terrilactibacillus laevilacticus NK26-11T was 98.3 %.
32519940	9	5	theme	gene	896:899	arg1	similarity					910:919	The 16S rRNA gene sequence similarity	883:919	The 16S rRNA gene sequence similarity between strain BCM23-1T and Terrilactibacillus laevilacticus NK26-11T	883:989	The 16S rRNA gene sequence similarity between strain BCM23-1T and Terrilactibacillus laevilacticus NK26-11T was 98.3 %.
32519940	12	6	theme	gene	1346:1349	arg1	similarity					1360:1369	16S rRNA gene sequence similarity	1337:1369	16S rRNA gene sequence similarity	1337:1369	The results of phenotypic and chemotaxonomic, 16S rRNA gene sequence similarity, and whole genome analyses support strain BCM23-1T as representing a novel species of Terrilactibacillus for which the name Terrilactibacillus tamarindi sp.
32519940	6	7	theme	major	522:526	arg1	menaquinone					551:561	menaquinone 7	551:563	menaquinone 7 (MK-7)	551:570	The major isoprenoid quinone was menaquinone 7 (MK-7).
32519940	6	7	theme	major	522:526	arg1	quinone					539:545	The major isoprenoid quinone	518:545	The major isoprenoid quinone	518:545	The major isoprenoid quinone was menaquinone 7 (MK-7).
32519940	2	8	theme	indica	240:245	arg1	bark					221:224	bark	221:224	bark of Tamarindus indica collected from Chiang Mai Province, Thailand	221:290	A Gram-stain-positive, catalase-positive, facultatively anaerobic, terminal-spore-forming rod, designated strain BCM23-1T, was isolated from bark of Tamarindus indica collected from Chiang Mai Province, Thailand.
32519940	12	9	theme	Terrilactibacillus	1495:1512	arg1	sp					1524:1525	the name Terrilactibacillus tamarindi sp	1486:1525	the name Terrilactibacillus tamarindi sp	1486:1525	The results of phenotypic and chemotaxonomic, 16S rRNA gene sequence similarity, and whole genome analyses support strain BCM23-1T as representing a novel species of Terrilactibacillus for which the name Terrilactibacillus tamarindi sp.
32519940	3	10	theme	d-lactic	314:321	arg1	acid					323:326	d-lactic acid	314:326	d-lactic acid from glucose	314:339	This strain produced d-lactic acid from glucose.
32519940	3	11	from	glucose	333:339	arg1	acid					323:326	d-lactic acid	314:326	d-lactic acid from glucose	314:339	This strain produced d-lactic acid from glucose.
32519940	14	12	theme	31662T=JCM	1580:1589	arg1	2841T					1604:1608	=LMG 31662T=JCM 33748T=TISTR 2841T	1575:1608	=LMG 31662T=JCM 33748T=TISTR 2841T	1575:1608	The type strain is BCM23-1T (=LMG 31662T=JCM 33748T=TISTR 2841T).
32519940	14	12	theme	31662T=JCM	1580:1589	arg1	BCM23-1T					1565:1572	BCM23-1T	1565:1572	BCM23-1T (=LMG 31662T=JCM 33748T=TISTR 2841T)	1565:1609	The type strain is BCM23-1T (=LMG 31662T=JCM 33748T=TISTR 2841T).
32519940	5	13	theme	cell-wall	452:460	arg1	peptidoglycan					462:474	The cell-wall peptidoglycan	448:474	The cell-wall peptidoglycan	448:474	The cell-wall peptidoglycan contained meso-diaminopimelic acid (A1γ).
32519940	10	14	theme	BCM23-1T	1023:1030	arg1	3.24 Mb					1036:1042	3.24 Mb	1036:1042	3.24 Mb	1036:1042	The draft genome of BCM23-1T was 3.24 Mb in size and contained 3088 coding sequences with an in silico DNA G+C content of 37.1 mol%.
32519940	10	14	theme	BCM23-1T	1023:1030	arg1	genome					1013:1018	The draft genome	1003:1018	The draft genome of BCM23-1T	1003:1030	The draft genome of BCM23-1T was 3.24 Mb in size and contained 3088 coding sequences with an in silico DNA G+C content of 37.1 mol%.
32519940	12	15	theme	novel	1440:1444	arg1	species					1446:1452	a novel species	1438:1452	a novel species of Terrilactibacillus for which the name Terrilactibacillus tamarindi sp	1438:1525	The results of phenotypic and chemotaxonomic, 16S rRNA gene sequence similarity, and whole genome analyses support strain BCM23-1T as representing a novel species of Terrilactibacillus for which the name Terrilactibacillus tamarindi sp.
32519940	2	16	dep	Gram-stain-positive	82:100	arg1	terminal-spore-forming					147:168	terminal-spore-forming	147:168	terminal-spore-forming	147:168	A Gram-stain-positive, catalase-positive, facultatively anaerobic, terminal-spore-forming rod, designated strain BCM23-1T, was isolated from bark of Tamarindus indica collected from Chiang Mai Province, Thailand.
32519940	2	16	dep	Gram-stain-positive	82:100	arg1	catalase-positive					103:119	catalase-positive	103:119	catalase-positive	103:119	A Gram-stain-positive, catalase-positive, facultatively anaerobic, terminal-spore-forming rod, designated strain BCM23-1T, was isolated from bark of Tamarindus indica collected from Chiang Mai Province, Thailand.
32519940	2	16	dep	Gram-stain-positive	82:100	arg1	anaerobic					136:144	anaerobic	136:144	anaerobic	136:144	A Gram-stain-positive, catalase-positive, facultatively anaerobic, terminal-spore-forming rod, designated strain BCM23-1T, was isolated from bark of Tamarindus indica collected from Chiang Mai Province, Thailand.
32519940	8	17	theme	cellular	773:780	arg1	anteiso-C17 					799:810	anteiso-C17 	799:810	anteiso-C17 : 0, anteiso-C15:0, and iso-C16 : 0	799:845	The predominant cellular fatty acids were anteiso-C17 : 0, anteiso-C15:0, and iso-C16 : 0 when cultivated on GYP agar plates.
32519940	8	17	theme	cellular	773:780	arg1	acids					788:792	The predominant cellular fatty acids	757:792	The predominant cellular fatty acids	757:792	The predominant cellular fatty acids were anteiso-C17 : 0, anteiso-C15:0, and iso-C16 : 0 when cultivated on GYP agar plates.
32519940	14	18	theme	type	1550:1553	arg1	strain					1555:1560	The type strain	1546:1560	The type strain	1546:1560	The type strain is BCM23-1T (=LMG 31662T=JCM 33748T=TISTR 2841T).
32519940	14	18	theme	type	1550:1553	arg1	BCM23-1T					1565:1572	BCM23-1T	1565:1572	BCM23-1T (=LMG 31662T=JCM 33748T=TISTR 2841T)	1565:1609	The type strain is BCM23-1T (=LMG 31662T=JCM 33748T=TISTR 2841T).
32519940	1	19	theme	indica	72:77	arg1	bark					53:56	bark	53:56	bark of Tamarindus indica	53:77	nov., isolated from bark of Tamarindus indica.
32519940	4	20	theme	1-4 	430:433	arg1	%					434:434	%	434:434	%	434:434	It grew at 20-45 °C (optimum, 30 °C), pH 3.5-9 (optimum, pH 7.0) and in the presence of 1-4 % (w/v) NaCl.
32519940	11	21	theme	T.	1223:1224	arg1	NK26-11T					1240:1247	T. laevilacticus NK26-11T	1223:1247	T. laevilacticus NK26-11T	1223:1247	The values of ANIb, ANIm and digital DNA-DNA hybridization between strain BCM23-1T and T. laevilacticus NK26-11T were 89.9, 90.8 and 40.4 %, respectively.
32519940	0	22	theme	Terrilactibacillus	0:17	arg1	sp					29:30	Terrilactibacillus tamarindi sp	0:30	Terrilactibacillus tamarindi sp.	0:31	Terrilactibacillus tamarindi sp.
32519940	4	23	dep	%	434:434	arg1	w/v					437:439	w/v	437:439	w/v	437:439	It grew at 20-45 °C (optimum, 30 °C), pH 3.5-9 (optimum, pH 7.0) and in the presence of 1-4 % (w/v) NaCl.
32519940	2	24	attach	isolated	207:214	arg2	rod					170:172	A Gram-stain-positive, catalase-positive, facultatively anaerobic, terminal-spore-forming rod	80:172	A Gram-stain-positive, catalase-positive, facultatively anaerobic, terminal-spore-forming rod	80:172	A Gram-stain-positive, catalase-positive, facultatively anaerobic, terminal-spore-forming rod, designated strain BCM23-1T, was isolated from bark of Tamarindus indica collected from Chiang Mai Province, Thailand.
32519940	2	24	attach	isolated	207:214	arg1	bark					221:224	bark	221:224	bark of Tamarindus indica collected from Chiang Mai Province, Thailand	221:290	A Gram-stain-positive, catalase-positive, facultatively anaerobic, terminal-spore-forming rod, designated strain BCM23-1T, was isolated from bark of Tamarindus indica collected from Chiang Mai Province, Thailand.
32519940	4	25	dep	20-45 °C	353:360	arg1	30 °C					372:376	30 °C	372:376	30 °C	372:376	It grew at 20-45 °C (optimum, 30 °C), pH 3.5-9 (optimum, pH 7.0) and in the presence of 1-4 % (w/v) NaCl.
32519940	4	25	dep	20-45 °C	353:360	arg1	optimum					363:369	optimum	363:369	optimum	363:369	It grew at 20-45 °C (optimum, 30 °C), pH 3.5-9 (optimum, pH 7.0) and in the presence of 1-4 % (w/v) NaCl.
32519940	10	26	theme	coding	1071:1076	arg1	sequences					1078:1086	3088 coding sequences	1066:1086	3088 coding sequences	1066:1086	The draft genome of BCM23-1T was 3.24 Mb in size and contained 3088 coding sequences with an in silico DNA G+C content of 37.1 mol%.
32519940	11	27	dep	T.	1223:1224	arg1	laevilacticus					1226:1238	laevilacticus	1226:1238	laevilacticus	1226:1238	The values of ANIb, ANIm and digital DNA-DNA hybridization between strain BCM23-1T and T. laevilacticus NK26-11T were 89.9, 90.8 and 40.4 %, respectively.
32519940	10	28	contain	contained	1056:1064	arg2	sequences					1078:1086	3088 coding sequences	1066:1086	3088 coding sequences	1066:1086	The draft genome of BCM23-1T was 3.24 Mb in size and contained 3088 coding sequences with an in silico DNA G+C content of 37.1 mol%.
32519940	10	28	contain	contained	1056:1064	arg1	3.24 Mb					1036:1042	3.24 Mb	1036:1042	3.24 Mb	1036:1042	The draft genome of BCM23-1T was 3.24 Mb in size and contained 3088 coding sequences with an in silico DNA G+C content of 37.1 mol%.
32519940	10	28	contain	contained	1056:1064	arg1	genome					1013:1018	The draft genome	1003:1018	The draft genome of BCM23-1T	1003:1030	The draft genome of BCM23-1T was 3.24 Mb in size and contained 3088 coding sequences with an in silico DNA G+C content of 37.1 mol%.
32519940	10	29	theme	DNA	1106:1108	arg1	content					1114:1120	an in silico DNA G+C content	1093:1120	an in silico DNA G+C content of 37.1 mol%	1093:1133	The draft genome of BCM23-1T was 3.24 Mb in size and contained 3088 coding sequences with an in silico DNA G+C content of 37.1 mol%.
32519940	8	30	theme	agar	870:873	arg1	plates					875:880	GYP agar plates	866:880	GYP agar plates	866:880	The predominant cellular fatty acids were anteiso-C17 : 0, anteiso-C15:0, and iso-C16 : 0 when cultivated on GYP agar plates.
32519940	7	31	theme	unidentified	737:748	arg1	lipid					750:754	an unidentified lipid	734:754	an unidentified lipid	734:754	Polar lipids analysis revealed the presence of diphosphatidylglycerol, phosphatidylglycerol, an unidentified aminophospholipid, an unidentified phospholipid and an unidentified lipid.
32519940	11	32	theme	DNA-DNA	1173:1179	arg1	hybridization					1181:1193	digital DNA-DNA hybridization	1165:1193	digital DNA-DNA hybridization	1165:1193	The values of ANIb, ANIm and digital DNA-DNA hybridization between strain BCM23-1T and T. laevilacticus NK26-11T were 89.9, 90.8 and 40.4 %, respectively.
32519940	10	33	theme	in	1096:1097	arg1	content					1114:1120	an in silico DNA G+C content	1093:1120	an in silico DNA G+C content of 37.1 mol%	1093:1133	The draft genome of BCM23-1T was 3.24 Mb in size and contained 3088 coding sequences with an in silico DNA G+C content of 37.1 mol%.
32519940	7	34	theme	Polar	573:577	arg1	analysis					586:593	Polar lipids analysis	573:593	Polar lipids analysis	573:593	Polar lipids analysis revealed the presence of diphosphatidylglycerol, phosphatidylglycerol, an unidentified aminophospholipid, an unidentified phospholipid and an unidentified lipid.
32519940	12	35	theme	analyses	1389:1396	arg1	results					1295:1301	The results	1291:1301	The results of phenotypic and chemotaxonomic, 16S rRNA gene sequence similarity, and whole genome analyses	1291:1396	The results of phenotypic and chemotaxonomic, 16S rRNA gene sequence similarity, and whole genome analyses support strain BCM23-1T as representing a novel species of Terrilactibacillus for which the name Terrilactibacillus tamarindi sp.
32519940	10	36	theme	37.1 mol	1125:1132	arg1	%					1133:1133	37.1 mol%	1125:1133	37.1 mol%	1125:1133	The draft genome of BCM23-1T was 3.24 Mb in size and contained 3088 coding sequences with an in silico DNA G+C content of 37.1 mol%.
32519940	12	37	theme	strain	1406:1411	arg1	BCM23-1T					1413:1420	strain BCM23-1T	1406:1420	strain BCM23-1T	1406:1420	The results of phenotypic and chemotaxonomic, 16S rRNA gene sequence similarity, and whole genome analyses support strain BCM23-1T as representing a novel species of Terrilactibacillus for which the name Terrilactibacillus tamarindi sp.
32519940	10	38	dep	in	1096:1097	arg1	silico					1099:1104	silico	1099:1104	silico	1099:1104	The draft genome of BCM23-1T was 3.24 Mb in size and contained 3088 coding sequences with an in silico DNA G+C content of 37.1 mol%.
32519940	14	39	theme	=LMG	1575:1578	arg1	2841T					1604:1608	=LMG 31662T=JCM 33748T=TISTR 2841T	1575:1608	=LMG 31662T=JCM 33748T=TISTR 2841T	1575:1608	The type strain is BCM23-1T (=LMG 31662T=JCM 33748T=TISTR 2841T).
32519940	14	39	theme	=LMG	1575:1578	arg1	BCM23-1T					1565:1572	BCM23-1T	1565:1572	BCM23-1T (=LMG 31662T=JCM 33748T=TISTR 2841T)	1565:1609	The type strain is BCM23-1T (=LMG 31662T=JCM 33748T=TISTR 2841T).
32519940	12	40	theme	whole	1376:1380	arg1	analyses					1389:1396	whole genome analyses	1376:1396	whole genome analyses	1376:1396	The results of phenotypic and chemotaxonomic, 16S rRNA gene sequence similarity, and whole genome analyses support strain BCM23-1T as representing a novel species of Terrilactibacillus for which the name Terrilactibacillus tamarindi sp.
32519940	12	41	theme	rRNA	1341:1344	arg1	similarity					1360:1369	16S rRNA gene sequence similarity	1337:1369	16S rRNA gene sequence similarity	1337:1369	The results of phenotypic and chemotaxonomic, 16S rRNA gene sequence similarity, and whole genome analyses support strain BCM23-1T as representing a novel species of Terrilactibacillus for which the name Terrilactibacillus tamarindi sp.
32519940	12	42	theme	phenotypic	1306:1315	arg1	similarity					1360:1369	16S rRNA gene sequence similarity	1337:1369	16S rRNA gene sequence similarity	1337:1369	The results of phenotypic and chemotaxonomic, 16S rRNA gene sequence similarity, and whole genome analyses support strain BCM23-1T as representing a novel species of Terrilactibacillus for which the name Terrilactibacillus tamarindi sp.
32519940	9	43	theme	rRNA	891:894	arg1	%					1000:1000	98.3 %	995:1000	98.3 %	995:1000	The 16S rRNA gene sequence similarity between strain BCM23-1T and Terrilactibacillus laevilacticus NK26-11T was 98.3 %.
32519940	9	43	theme	rRNA	891:894	arg1	similarity					910:919	The 16S rRNA gene sequence similarity	883:919	The 16S rRNA gene sequence similarity between strain BCM23-1T and Terrilactibacillus laevilacticus NK26-11T	883:989	The 16S rRNA gene sequence similarity between strain BCM23-1T and Terrilactibacillus laevilacticus NK26-11T was 98.3 %.
32519940	12	44	theme	sequence	1351:1358	arg1	similarity					1360:1369	16S rRNA gene sequence similarity	1337:1369	16S rRNA gene sequence similarity	1337:1369	The results of phenotypic and chemotaxonomic, 16S rRNA gene sequence similarity, and whole genome analyses support strain BCM23-1T as representing a novel species of Terrilactibacillus for which the name Terrilactibacillus tamarindi sp.
32519940	12	45	theme	chemotaxonomic	1321:1334	arg1	results					1295:1301	The results	1291:1301	The results of phenotypic and chemotaxonomic, 16S rRNA gene sequence similarity, and whole genome analyses	1291:1396	The results of phenotypic and chemotaxonomic, 16S rRNA gene sequence similarity, and whole genome analyses support strain BCM23-1T as representing a novel species of Terrilactibacillus for which the name Terrilactibacillus tamarindi sp.
32519940	9	46	theme	sequence	901:908	arg1	%					1000:1000	98.3 %	995:1000	98.3 %	995:1000	The 16S rRNA gene sequence similarity between strain BCM23-1T and Terrilactibacillus laevilacticus NK26-11T was 98.3 %.
32519940	9	46	theme	sequence	901:908	arg1	similarity					910:919	The 16S rRNA gene sequence similarity	883:919	The 16S rRNA gene sequence similarity between strain BCM23-1T and Terrilactibacillus laevilacticus NK26-11T	883:989	The 16S rRNA gene sequence similarity between strain BCM23-1T and Terrilactibacillus laevilacticus NK26-11T was 98.3 %.
32519940	11	47	theme	89.9	1254:1257	arg1	%					1274:1274	89.9, 90.8 and 40.4 %	1254:1274	%	1274:1274	The values of ANIb, ANIm and digital DNA-DNA hybridization between strain BCM23-1T and T. laevilacticus NK26-11T were 89.9, 90.8 and 40.4 %, respectively.
32519940	11	47	theme	89.9	1254:1257	arg1	values					1140:1145	The values	1136:1145	The values of ANIb, ANIm and digital DNA-DNA hybridization between strain BCM23-1T and T. laevilacticus NK26-11T	1136:1247	The values of ANIb, ANIm and digital DNA-DNA hybridization between strain BCM23-1T and T. laevilacticus NK26-11T were 89.9, 90.8 and 40.4 %, respectively.
32519940	7	48	theme	phosphatidylglycerol	644:663	arg1	presence					608:615	the presence	604:615	the presence of diphosphatidylglycerol, phosphatidylglycerol, an unidentified aminophospholipid, an unidentified phospholipid and an unidentified lipid	604:754	Polar lipids analysis revealed the presence of diphosphatidylglycerol, phosphatidylglycerol, an unidentified aminophospholipid, an unidentified phospholipid and an unidentified lipid.
32519940	12	49	theme	tamarindi	1514:1522	arg1	sp					1524:1525	the name Terrilactibacillus tamarindi sp	1486:1525	the name Terrilactibacillus tamarindi sp	1486:1525	The results of phenotypic and chemotaxonomic, 16S rRNA gene sequence similarity, and whole genome analyses support strain BCM23-1T as representing a novel species of Terrilactibacillus for which the name Terrilactibacillus tamarindi sp.
32519940	6	50	theme	isoprenoid	528:537	arg1	menaquinone					551:561	menaquinone 7	551:563	menaquinone 7 (MK-7)	551:570	The major isoprenoid quinone was menaquinone 7 (MK-7).
32519940	6	50	theme	isoprenoid	528:537	arg1	quinone					539:545	The major isoprenoid quinone	518:545	The major isoprenoid quinone	518:545	The major isoprenoid quinone was menaquinone 7 (MK-7).
32519940	9	51	theme	Terrilactibacillus	949:966	arg1	NK26-11T					982:989	Terrilactibacillus laevilacticus NK26-11T	949:989	Terrilactibacillus laevilacticus NK26-11T	949:989	The 16S rRNA gene sequence similarity between strain BCM23-1T and Terrilactibacillus laevilacticus NK26-11T was 98.3 %.
32519940	2	52	theme	Gram-stain-positive	82:100	arg1	rod					170:172	A Gram-stain-positive, catalase-positive, facultatively anaerobic, terminal-spore-forming rod	80:172	A Gram-stain-positive, catalase-positive, facultatively anaerobic, terminal-spore-forming rod	80:172	A Gram-stain-positive, catalase-positive, facultatively anaerobic, terminal-spore-forming rod, designated strain BCM23-1T, was isolated from bark of Tamarindus indica collected from Chiang Mai Province, Thailand.
32519940	7	53	theme	diphosphatidylglycerol	620:641	arg1	presence					608:615	the presence	604:615	the presence of diphosphatidylglycerol, phosphatidylglycerol, an unidentified aminophospholipid, an unidentified phospholipid and an unidentified lipid	604:754	Polar lipids analysis revealed the presence of diphosphatidylglycerol, phosphatidylglycerol, an unidentified aminophospholipid, an unidentified phospholipid and an unidentified lipid.
32519940	2	54	theme	Tamarindus	229:238	arg1	indica					240:245	Tamarindus indica	229:245	Tamarindus indica collected from Chiang Mai Province, Thailand	229:290	A Gram-stain-positive, catalase-positive, facultatively anaerobic, terminal-spore-forming rod, designated strain BCM23-1T, was isolated from bark of Tamarindus indica collected from Chiang Mai Province, Thailand.
32519940	5	55	theme	meso-diaminopimelic	486:504	arg1	A1γ					512:514	A1γ	512:514	A1γ	512:514	The cell-wall peptidoglycan contained meso-diaminopimelic acid (A1γ).
32519940	5	55	theme	meso-diaminopimelic	486:504	arg1	acid					506:509	meso-diaminopimelic acid	486:509	meso-diaminopimelic acid (A1γ)	486:515	The cell-wall peptidoglycan contained meso-diaminopimelic acid (A1γ).
32519940	10	56	theme	draft	1007:1011	arg1	3.24 Mb					1036:1042	3.24 Mb	1036:1042	3.24 Mb	1036:1042	The draft genome of BCM23-1T was 3.24 Mb in size and contained 3088 coding sequences with an in silico DNA G+C content of 37.1 mol%.
32519940	10	56	theme	draft	1007:1011	arg1	genome					1013:1018	The draft genome	1003:1018	The draft genome of BCM23-1T	1003:1030	The draft genome of BCM23-1T was 3.24 Mb in size and contained 3088 coding sequences with an in silico DNA G+C content of 37.1 mol%.
32519940	8	57	theme	fatty	782:786	arg1	anteiso-C17 					799:810	anteiso-C17 	799:810	anteiso-C17 : 0, anteiso-C15:0, and iso-C16 : 0	799:845	The predominant cellular fatty acids were anteiso-C17 : 0, anteiso-C15:0, and iso-C16 : 0 when cultivated on GYP agar plates.
32519940	8	57	theme	fatty	782:786	arg1	acids					788:792	The predominant cellular fatty acids	757:792	The predominant cellular fatty acids	757:792	The predominant cellular fatty acids were anteiso-C17 : 0, anteiso-C15:0, and iso-C16 : 0 when cultivated on GYP agar plates.
32519940	5	58	contain	contained	476:484	arg2	A1γ					512:514	A1γ	512:514	A1γ	512:514	The cell-wall peptidoglycan contained meso-diaminopimelic acid (A1γ).
32519940	5	58	contain	contained	476:484	arg1	peptidoglycan					462:474	The cell-wall peptidoglycan	448:474	The cell-wall peptidoglycan	448:474	The cell-wall peptidoglycan contained meso-diaminopimelic acid (A1γ).
32519940	5	58	contain	contained	476:484	arg2	acid					506:509	meso-diaminopimelic acid	486:509	meso-diaminopimelic acid (A1γ)	486:515	The cell-wall peptidoglycan contained meso-diaminopimelic acid (A1γ).
32519940	14	59	theme	33748T=TISTR	1591:1602	arg1	2841T					1604:1608	=LMG 31662T=JCM 33748T=TISTR 2841T	1575:1608	=LMG 31662T=JCM 33748T=TISTR 2841T	1575:1608	The type strain is BCM23-1T (=LMG 31662T=JCM 33748T=TISTR 2841T).
32519940	14	59	theme	33748T=TISTR	1591:1602	arg1	BCM23-1T					1565:1572	BCM23-1T	1565:1572	BCM23-1T (=LMG 31662T=JCM 33748T=TISTR 2841T)	1565:1609	The type strain is BCM23-1T (=LMG 31662T=JCM 33748T=TISTR 2841T).
32519940	12	60	theme	name	1490:1493	arg1	sp					1524:1525	the name Terrilactibacillus tamarindi sp	1486:1525	the name Terrilactibacillus tamarindi sp	1486:1525	The results of phenotypic and chemotaxonomic, 16S rRNA gene sequence similarity, and whole genome analyses support strain BCM23-1T as representing a novel species of Terrilactibacillus for which the name Terrilactibacillus tamarindi sp.
32519940	9	61	theme	strain	929:934	arg1	BCM23-1T					936:943	strain BCM23-1T	929:943	strain BCM23-1T	929:943	The 16S rRNA gene sequence similarity between strain BCM23-1T and Terrilactibacillus laevilacticus NK26-11T was 98.3 %.
32519940	7	62	theme	unidentified	704:715	arg1	phospholipid					717:728	an unidentified phospholipid	701:728	an unidentified phospholipid	701:728	Polar lipids analysis revealed the presence of diphosphatidylglycerol, phosphatidylglycerol, an unidentified aminophospholipid, an unidentified phospholipid and an unidentified lipid.
32519940	7	63	theme	lipids	579:584	arg1	analysis					586:593	Polar lipids analysis	573:593	Polar lipids analysis	573:593	Polar lipids analysis revealed the presence of diphosphatidylglycerol, phosphatidylglycerol, an unidentified aminophospholipid, an unidentified phospholipid and an unidentified lipid.
32519940	4	64	theme	NaCl	442:445	arg1	presence					418:425	the presence	414:425	the presence of 1-4 % (w/v) NaCl	414:445	It grew at 20-45 °C (optimum, 30 °C), pH 3.5-9 (optimum, pH 7.0) and in the presence of 1-4 % (w/v) NaCl.
32519940	12	65	theme	Terrilactibacillus	1457:1474	arg1	species					1446:1452	a novel species	1438:1452	a novel species of Terrilactibacillus for which the name Terrilactibacillus tamarindi sp	1438:1525	The results of phenotypic and chemotaxonomic, 16S rRNA gene sequence similarity, and whole genome analyses support strain BCM23-1T as representing a novel species of Terrilactibacillus for which the name Terrilactibacillus tamarindi sp.
32519940	11	66	theme	strain	1203:1208	arg1	BCM23-1T					1210:1217	strain BCM23-1T	1203:1217	strain BCM23-1T	1203:1217	The values of ANIb, ANIm and digital DNA-DNA hybridization between strain BCM23-1T and T. laevilacticus NK26-11T were 89.9, 90.8 and 40.4 %, respectively.
32519940	8	67	theme	predominant	761:771	arg1	anteiso-C17 					799:810	anteiso-C17 	799:810	anteiso-C17 : 0, anteiso-C15:0, and iso-C16 : 0	799:845	The predominant cellular fatty acids were anteiso-C17 : 0, anteiso-C15:0, and iso-C16 : 0 when cultivated on GYP agar plates.
32519940	8	67	theme	predominant	761:771	arg1	acids					788:792	The predominant cellular fatty acids	757:792	The predominant cellular fatty acids	757:792	The predominant cellular fatty acids were anteiso-C17 : 0, anteiso-C15:0, and iso-C16 : 0 when cultivated on GYP agar plates.
32519940	9	68	dep	Terrilactibacillus	949:966	arg1	laevilacticus					968:980	laevilacticus	968:980	laevilacticus	968:980	The 16S rRNA gene sequence similarity between strain BCM23-1T and Terrilactibacillus laevilacticus NK26-11T was 98.3 %.
32519940	1	69	theme	Tamarindus	61:70	arg1	indica					72:77	Tamarindus indica	61:77	Tamarindus indica	61:77	nov., isolated from bark of Tamarindus indica.
32519940	4	70	theme	%	434:434	arg1	NaCl					442:445	1-4 % (w/v) NaCl	430:445	1-4 % (w/v) NaCl	430:445	It grew at 20-45 °C (optimum, 30 °C), pH 3.5-9 (optimum, pH 7.0) and in the presence of 1-4 % (w/v) NaCl.
32519940	0	71	theme	tamarindi	19:27	arg1	sp					29:30	Terrilactibacillus tamarindi sp	0:30	Terrilactibacillus tamarindi sp.	0:31	Terrilactibacillus tamarindi sp.
32519940	10	72	from	3.24 Mb	1036:1042	arg1	size					1047:1050	size	1047:1050	size	1047:1050	The draft genome of BCM23-1T was 3.24 Mb in size and contained 3088 coding sequences with an in silico DNA G+C content of 37.1 mol%.
32519940	11	73	theme	ANIm	1156:1159	arg1	%					1274:1274	89.9, 90.8 and 40.4 %	1254:1274	%	1274:1274	The values of ANIb, ANIm and digital DNA-DNA hybridization between strain BCM23-1T and T. laevilacticus NK26-11T were 89.9, 90.8 and 40.4 %, respectively.
32519940	11	73	theme	ANIm	1156:1159	arg1	values					1140:1145	The values	1136:1145	The values of ANIb, ANIm and digital DNA-DNA hybridization between strain BCM23-1T and T. laevilacticus NK26-11T	1136:1247	The values of ANIb, ANIm and digital DNA-DNA hybridization between strain BCM23-1T and T. laevilacticus NK26-11T were 89.9, 90.8 and 40.4 %, respectively.
32519940	10	74	from	size	1047:1050	arg1	3.24 Mb					1036:1042	3.24 Mb	1036:1042	3.24 Mb	1036:1042	The draft genome of BCM23-1T was 3.24 Mb in size and contained 3088 coding sequences with an in silico DNA G+C content of 37.1 mol%.
32519940	10	74	from	size	1047:1050	arg1	genome					1013:1018	The draft genome	1003:1018	The draft genome of BCM23-1T	1003:1030	The draft genome of BCM23-1T was 3.24 Mb in size and contained 3088 coding sequences with an in silico DNA G+C content of 37.1 mol%.
32519940	11	75	theme	ANIb	1150:1153	arg1	%					1274:1274	89.9, 90.8 and 40.4 %	1254:1274	%	1274:1274	The values of ANIb, ANIm and digital DNA-DNA hybridization between strain BCM23-1T and T. laevilacticus NK26-11T were 89.9, 90.8 and 40.4 %, respectively.
32519940	11	75	theme	ANIb	1150:1153	arg1	values					1140:1145	The values	1136:1145	The values of ANIb, ANIm and digital DNA-DNA hybridization between strain BCM23-1T and T. laevilacticus NK26-11T	1136:1247	The values of ANIb, ANIm and digital DNA-DNA hybridization between strain BCM23-1T and T. laevilacticus NK26-11T were 89.9, 90.8 and 40.4 %, respectively.
32519940	2	76	theme	strain	186:191	arg1	BCM23-1T					193:200	strain BCM23-1T	186:200	strain BCM23-1T	186:200	A Gram-stain-positive, catalase-positive, facultatively anaerobic, terminal-spore-forming rod, designated strain BCM23-1T, was isolated from bark of Tamarindus indica collected from Chiang Mai Province, Thailand.
32519940	7	77	theme	lipid	750:754	arg1	presence					608:615	the presence	604:615	the presence of diphosphatidylglycerol, phosphatidylglycerol, an unidentified aminophospholipid, an unidentified phospholipid and an unidentified lipid	604:754	Polar lipids analysis revealed the presence of diphosphatidylglycerol, phosphatidylglycerol, an unidentified aminophospholipid, an unidentified phospholipid and an unidentified lipid.
32519940	8	78	theme	GYP	866:868	arg1	plates					875:880	GYP agar plates	866:880	GYP agar plates	866:880	The predominant cellular fatty acids were anteiso-C17 : 0, anteiso-C15:0, and iso-C16 : 0 when cultivated on GYP agar plates.
32519940	11	79	theme	hybridization	1181:1193	arg1	%					1274:1274	89.9, 90.8 and 40.4 %	1254:1274	%	1274:1274	The values of ANIb, ANIm and digital DNA-DNA hybridization between strain BCM23-1T and T. laevilacticus NK26-11T were 89.9, 90.8 and 40.4 %, respectively.
32519940	11	79	theme	hybridization	1181:1193	arg1	values					1140:1145	The values	1136:1145	The values of ANIb, ANIm and digital DNA-DNA hybridization between strain BCM23-1T and T. laevilacticus NK26-11T	1136:1247	The values of ANIb, ANIm and digital DNA-DNA hybridization between strain BCM23-1T and T. laevilacticus NK26-11T were 89.9, 90.8 and 40.4 %, respectively.
32519940	12	80	theme	genome	1382:1387	arg1	analyses					1389:1396	whole genome analyses	1376:1396	whole genome analyses	1376:1396	The results of phenotypic and chemotaxonomic, 16S rRNA gene sequence similarity, and whole genome analyses support strain BCM23-1T as representing a novel species of Terrilactibacillus for which the name Terrilactibacillus tamarindi sp.
32519940	11	81	theme	digital	1165:1171	arg1	hybridization					1181:1193	digital DNA-DNA hybridization	1165:1193	digital DNA-DNA hybridization	1165:1193	The values of ANIb, ANIm and digital DNA-DNA hybridization between strain BCM23-1T and T. laevilacticus NK26-11T were 89.9, 90.8 and 40.4 %, respectively.
32519940	8	82	dep	anteiso-C17 	799:810	arg1	iso-C16 					835:842	iso-C16 	835:842	iso-C16 	835:842	The predominant cellular fatty acids were anteiso-C17 : 0, anteiso-C15:0, and iso-C16 : 0 when cultivated on GYP agar plates.
32519940	8	82	dep	anteiso-C17 	799:810	arg1	 0					812:813	 0	812:813	 0	812:813	The predominant cellular fatty acids were anteiso-C17 : 0, anteiso-C15:0, and iso-C16 : 0 when cultivated on GYP agar plates.
32519940	8	82	dep	anteiso-C17 	799:810	arg1	 0					844:845	 0	844:845	anteiso-C17 : 0, anteiso-C15:0, and iso-C16 : 0	799:845	The predominant cellular fatty acids were anteiso-C17 : 0, anteiso-C15:0, and iso-C16 : 0 when cultivated on GYP agar plates.
32519940	8	82	dep	anteiso-C17 	799:810	arg1	anteiso-C15:0					816:828	anteiso-C15:0	816:828	anteiso-C15:0	816:828	The predominant cellular fatty acids were anteiso-C17 : 0, anteiso-C15:0, and iso-C16 : 0 when cultivated on GYP agar plates.
32519940	7	83	theme	phospholipid	717:728	arg1	presence					608:615	the presence	604:615	the presence of diphosphatidylglycerol, phosphatidylglycerol, an unidentified aminophospholipid, an unidentified phospholipid and an unidentified lipid	604:754	Polar lipids analysis revealed the presence of diphosphatidylglycerol, phosphatidylglycerol, an unidentified aminophospholipid, an unidentified phospholipid and an unidentified lipid.
32519940	11	84	theme	40.4 	1269:1273	arg1	%					1274:1274	89.9, 90.8 and 40.4 %	1254:1274	%	1274:1274	The values of ANIb, ANIm and digital DNA-DNA hybridization between strain BCM23-1T and T. laevilacticus NK26-11T were 89.9, 90.8 and 40.4 %, respectively.
32519940	11	84	theme	40.4 	1269:1273	arg1	values					1140:1145	The values	1136:1145	The values of ANIb, ANIm and digital DNA-DNA hybridization between strain BCM23-1T and T. laevilacticus NK26-11T	1136:1247	The values of ANIb, ANIm and digital DNA-DNA hybridization between strain BCM23-1T and T. laevilacticus NK26-11T were 89.9, 90.8 and 40.4 %, respectively.
32519940	4	85	dep	optimum	390:396	arg1	pH					399:400	pH 7.0	399:404	pH 7.0	399:404	It grew at 20-45 °C (optimum, 30 °C), pH 3.5-9 (optimum, pH 7.0) and in the presence of 1-4 % (w/v) NaCl.
32519940	10	86	theme	G+C	1110:1112	arg1	content					1114:1120	an in silico DNA G+C content	1093:1120	an in silico DNA G+C content of 37.1 mol%	1093:1133	The draft genome of BCM23-1T was 3.24 Mb in size and contained 3088 coding sequences with an in silico DNA G+C content of 37.1 mol%.
32375934	12	0	dep	data	1393:1396	arg1	basis					1371:1375	basis	1371:1375	basis	1371:1375	On the basis of phylogenetic data and several distinct phenotypic characteristics, strain JLT9T represents a novel species of the genus Serinicoccus, for which the name Serinicoccus hydrothermalis sp.
32375934	12	0	dep	data	1393:1396	arg1	the					1367:1369	the	1367:1369	the	1367:1369	On the basis of phylogenetic data and several distinct phenotypic characteristics, strain JLT9T represents a novel species of the genus Serinicoccus, for which the name Serinicoccus hydrothermalis sp.
32375934	4	1	theme	rRNA	457:460	arg1	similarity					476:485	highest 16S rRNA gene sequence similarity	445:485	highest 16S rRNA gene sequence similarity to Serinicoccus marinus DSM 15273T (98.83 %)	445:530	Strain JLT9T exhibited highest 16S rRNA gene sequence similarity to Serinicoccus marinus DSM 15273T (98.83 %).
32375934	5	2	theme	strain	599:604	arg1	JLT9T					606:610	strain JLT9T	599:610	strain JLT9T	599:610	Phylogenetic trees based on 16S rRNA gene sequences revealed that strain JLT9T belonged to the genus Serinicoccus, clustering with Serinicoccus marinus JC1078T, Serinicoccus profundi MCCC 1A05965T, Serinicoccus sediminis GP-T3-3T and Serinicoccus chungangensis CAU9536T.
32375934	6	3	theme	related	889:895	arg1	strain					897:902	the closest related strain	877:902	the closest related strain	877:902	The digital DNA-DNA genome hybridization values between strain JLT9T and the closest related strain S. marinus DSM 15273T was 34.30 %.
32375934	6	4	dep	S.	904:905	arg1	marinus					907:913	marinus	907:913	marinus	907:913	The digital DNA-DNA genome hybridization values between strain JLT9T and the closest related strain S. marinus DSM 15273T was 34.30 %.
32375934	12	5	theme	phylogenetic	1380:1391	arg1	data					1393:1396	phylogenetic data	1380:1396	phylogenetic data	1380:1396	On the basis of phylogenetic data and several distinct phenotypic characteristics, strain JLT9T represents a novel species of the genus Serinicoccus, for which the name Serinicoccus hydrothermalis sp.
32375934	4	6	dep	Serinicoccus	490:501	arg1	marinus					503:509	marinus	503:509	marinus	503:509	Strain JLT9T exhibited highest 16S rRNA gene sequence similarity to Serinicoccus marinus DSM 15273T (98.83 %).
32375934	9	7	theme	unidentified	1213:1224	arg1	phospholipid					1226:1237	an unidentified phospholipid	1210:1237	an unidentified phospholipid	1210:1237	The polar lipids of strain JLT9T comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, three unidentified glycolipid and an unidentified phospholipid.
32375934	7	8	theme	DNA	943:945	arg1	content					951:957	The DNA G+C content	939:957	The DNA G+C content	939:957	The DNA G+C content was 72.43 mol%.
32375934	7	8	theme	DNA	943:945	arg1	%					972:972	72.43 mol%	963:972	72.43 mol%	963:972	The DNA G+C content was 72.43 mol%.
32375934	1	9	dep	Island	101:106	arg1	off					85:87	off	85:87	off	85:87	nov., isolated from shallow-sea hydrothermal systems off Kueishantao Island.
32375934	12	10	theme	several	1402:1408	arg1	characteristics					1430:1444	several distinct phenotypic characteristics	1402:1444	several distinct phenotypic characteristics	1402:1444	On the basis of phylogenetic data and several distinct phenotypic characteristics, strain JLT9T represents a novel species of the genus Serinicoccus, for which the name Serinicoccus hydrothermalis sp.
32375934	4	11	theme	sequence	467:474	arg1	similarity					476:485	highest 16S rRNA gene sequence similarity	445:485	highest 16S rRNA gene sequence similarity to Serinicoccus marinus DSM 15273T (98.83 %)	445:530	Strain JLT9T exhibited highest 16S rRNA gene sequence similarity to Serinicoccus marinus DSM 15273T (98.83 %).
32375934	7	12	theme	G+C	947:949	arg1	content					951:957	The DNA G+C content	939:957	The DNA G+C content	939:957	The DNA G+C content was 72.43 mol%.
32375934	7	12	theme	G+C	947:949	arg1	%					972:972	72.43 mol%	963:972	72.43 mol%	963:972	The DNA G+C content was 72.43 mol%.
32375934	1	13	theme	shallow-sea	52:62	arg1	systems					77:83	shallow-sea hydrothermal systems	52:83	shallow-sea hydrothermal systems off Kueishantao Island	52:106	nov., isolated from shallow-sea hydrothermal systems off Kueishantao Island.
32375934	12	14	dep	Serinicoccus	1533:1544	arg1	hydrothermalis					1546:1559	hydrothermalis	1546:1559	hydrothermalis	1546:1559	On the basis of phylogenetic data and several distinct phenotypic characteristics, strain JLT9T represents a novel species of the genus Serinicoccus, for which the name Serinicoccus hydrothermalis sp.
32375934	2	15	theme	bacterial	170:178	arg1	strain					180:185	A Gram-stain-positive, non-flagellated, non-gliding, coccoid bacterial strain	109:185	A Gram-stain-positive, non-flagellated, non-gliding, coccoid bacterial strain	109:185	A Gram-stain-positive, non-flagellated, non-gliding, coccoid bacterial strain, designated JLT9T, was isolated from the shallow-sea hydrothermal system off Kueishantao Island, Taiwan, ROC.
32375934	5	16	theme	16S	561:563	arg1	sequences					575:583	16S rRNA gene sequences	561:583	16S rRNA gene sequences	561:583	Phylogenetic trees based on 16S rRNA gene sequences revealed that strain JLT9T belonged to the genus Serinicoccus, clustering with Serinicoccus marinus JC1078T, Serinicoccus profundi MCCC 1A05965T, Serinicoccus sediminis GP-T3-3T and Serinicoccus chungangensis CAU9536T.
32375934	1	17	theme	hydrothermal	64:75	arg1	systems					77:83	shallow-sea hydrothermal systems	52:83	shallow-sea hydrothermal systems off Kueishantao Island	52:106	nov., isolated from shallow-sea hydrothermal systems off Kueishantao Island.
32375934	10	18	theme	isoprenoid	1256:1265	arg1	MK-8					1279:1282	MK-8	1279:1282	MK-8 (H4)	1279:1287	The predominant isoprenoid quinone was MK-8 (H4).
32375934	10	18	theme	isoprenoid	1256:1265	arg1	quinone					1267:1273	The predominant isoprenoid quinone	1240:1273	The predominant isoprenoid quinone	1240:1273	The predominant isoprenoid quinone was MK-8 (H4).
32375934	6	19	theme	closest	881:887	arg1	strain					897:902	the closest related strain	877:902	the closest related strain	877:902	The digital DNA-DNA genome hybridization values between strain JLT9T and the closest related strain S. marinus DSM 15273T was 34.30 %.
32375934	5	20	theme	gene	570:573	arg1	sequences					575:583	16S rRNA gene sequences	561:583	16S rRNA gene sequences	561:583	Phylogenetic trees based on 16S rRNA gene sequences revealed that strain JLT9T belonged to the genus Serinicoccus, clustering with Serinicoccus marinus JC1078T, Serinicoccus profundi MCCC 1A05965T, Serinicoccus sediminis GP-T3-3T and Serinicoccus chungangensis CAU9536T.
32375934	5	21	theme	rRNA	565:568	arg1	sequences					575:583	16S rRNA gene sequences	561:583	16S rRNA gene sequences	561:583	Phylogenetic trees based on 16S rRNA gene sequences revealed that strain JLT9T belonged to the genus Serinicoccus, clustering with Serinicoccus marinus JC1078T, Serinicoccus profundi MCCC 1A05965T, Serinicoccus sediminis GP-T3-3T and Serinicoccus chungangensis CAU9536T.
32375934	10	22	theme	predominant	1244:1254	arg1	MK-8					1279:1282	MK-8	1279:1282	MK-8 (H4)	1279:1287	The predominant isoprenoid quinone was MK-8 (H4).
32375934	10	22	theme	predominant	1244:1254	arg1	quinone					1267:1273	The predominant isoprenoid quinone	1240:1273	The predominant isoprenoid quinone	1240:1273	The predominant isoprenoid quinone was MK-8 (H4).
32375934	2	23	attach	isolated	210:217	arg2	strain					180:185	A Gram-stain-positive, non-flagellated, non-gliding, coccoid bacterial strain	109:185	A Gram-stain-positive, non-flagellated, non-gliding, coccoid bacterial strain	109:185	A Gram-stain-positive, non-flagellated, non-gliding, coccoid bacterial strain, designated JLT9T, was isolated from the shallow-sea hydrothermal system off Kueishantao Island, Taiwan, ROC.
32375934	2	23	attach	isolated	210:217	arg1	system					253:258	the shallow-sea hydrothermal system	224:258	the shallow-sea hydrothermal system	224:258	A Gram-stain-positive, non-flagellated, non-gliding, coccoid bacterial strain, designated JLT9T, was isolated from the shallow-sea hydrothermal system off Kueishantao Island, Taiwan, ROC.
32375934	11	24	theme	diaminopimelic	1343:1356	arg1	acid					1358:1361	no diaminopimelic acid	1340:1361	no diaminopimelic acid	1340:1361	The cell wall contained ornithine and serine, and no diaminopimelic acid.
32375934	4	25	theme	16S	453:455	arg1	similarity					476:485	highest 16S rRNA gene sequence similarity	445:485	highest 16S rRNA gene sequence similarity to Serinicoccus marinus DSM 15273T (98.83 %)	445:530	Strain JLT9T exhibited highest 16S rRNA gene sequence similarity to Serinicoccus marinus DSM 15273T (98.83 %).
32375934	14	26	theme	1.15779T=JCM	1616:1627	arg1	31502T					1629:1634	=CGMCC 1.15779T=JCM 31502T	1609:1634	=CGMCC 1.15779T=JCM 31502T	1609:1634	The type strain is JLT9T (=CGMCC 1.15779T=JCM 31502T).
32375934	14	26	theme	1.15779T=JCM	1616:1627	arg1	JLT9T					1602:1606	JLT9T	1602:1606	JLT9T (=CGMCC 1.15779T=JCM 31502T)	1602:1635	The type strain is JLT9T (=CGMCC 1.15779T=JCM 31502T).
32375934	6	27	theme	DSM	915:917	arg1	15273T					919:924	S. marinus DSM 15273T	904:924	S. marinus DSM 15273T	904:924	The digital DNA-DNA genome hybridization values between strain JLT9T and the closest related strain S. marinus DSM 15273T was 34.30 %.
32375934	0	28	theme	hydrothermalis	13:26	arg1	sp					28:29	Serinicoccus hydrothermalis sp	0:29	Serinicoccus hydrothermalis sp.	0:30	Serinicoccus hydrothermalis sp.
32375934	4	29	theme	highest	445:451	arg1	similarity					476:485	highest 16S rRNA gene sequence similarity	445:485	highest 16S rRNA gene sequence similarity to Serinicoccus marinus DSM 15273T (98.83 %)	445:530	Strain JLT9T exhibited highest 16S rRNA gene sequence similarity to Serinicoccus marinus DSM 15273T (98.83 %).
32375934	1	30	theme	Kueishantao	89:99	arg1	Island					101:106	Kueishantao Island	89:106	Kueishantao Island	89:106	nov., isolated from shallow-sea hydrothermal systems off Kueishantao Island.
32375934	3	31	theme	2.5 	404:407	arg1	%					408:408	%	408:408	%	408:408	Strain JLT9T was aerobic, chemoheterotrophic and grew optimally at 35 °C, at pH 6.0 and in the presence of 2.5 % (w/v) NaCl.
32375934	0	32	theme	Serinicoccus	0:11	arg1	sp					28:29	Serinicoccus hydrothermalis sp	0:29	Serinicoccus hydrothermalis sp.	0:30	Serinicoccus hydrothermalis sp.
32375934	5	33	theme	Phylogenetic	533:544	arg1	trees					546:550	Phylogenetic trees	533:550	Phylogenetic trees based on 16S rRNA gene sequences	533:583	Phylogenetic trees based on 16S rRNA gene sequences revealed that strain JLT9T belonged to the genus Serinicoccus, clustering with Serinicoccus marinus JC1078T, Serinicoccus profundi MCCC 1A05965T, Serinicoccus sediminis GP-T3-3T and Serinicoccus chungangensis CAU9536T.
32375934	12	34	theme	phenotypic	1419:1428	arg1	characteristics					1430:1444	several distinct phenotypic characteristics	1402:1444	several distinct phenotypic characteristics	1402:1444	On the basis of phylogenetic data and several distinct phenotypic characteristics, strain JLT9T represents a novel species of the genus Serinicoccus, for which the name Serinicoccus hydrothermalis sp.
32375934	3	35	theme	%	408:408	arg1	NaCl					416:419	2.5 % (w/v) NaCl	404:419	2.5 % (w/v) NaCl	404:419	Strain JLT9T was aerobic, chemoheterotrophic and grew optimally at 35 °C, at pH 6.0 and in the presence of 2.5 % (w/v) NaCl.
32375934	8	36	theme	fatty	988:992	arg1	 0					1028:1029	 0	1028:1029	 0	1028:1029	The dominant fatty acids were identified as iso-C15 : 0 (41.4 %) and iso-C16 : 0 (24.7 %).
32375934	8	36	theme	fatty	988:992	arg1	acids					994:998	The dominant fatty acids	975:998	The dominant fatty acids	975:998	The dominant fatty acids were identified as iso-C15 : 0 (41.4 %) and iso-C16 : 0 (24.7 %).
32375934	8	36	theme	fatty	988:992	arg1	 0					1053:1054	 0	1053:1054	 0	1053:1054	The dominant fatty acids were identified as iso-C15 : 0 (41.4 %) and iso-C16 : 0 (24.7 %).
32375934	8	36	theme	fatty	988:992	arg1	iso-C16 					1044:1051	iso-C16 	1044:1051	iso-C16 	1044:1051	The dominant fatty acids were identified as iso-C15 : 0 (41.4 %) and iso-C16 : 0 (24.7 %).
32375934	8	36	theme	fatty	988:992	arg1	iso-C15 					1019:1026	iso-C15 	1019:1026	iso-C15 	1019:1026	The dominant fatty acids were identified as iso-C15 : 0 (41.4 %) and iso-C16 : 0 (24.7 %).
32375934	8	37	theme	dominant	979:986	arg1	 0					1028:1029	 0	1028:1029	 0	1028:1029	The dominant fatty acids were identified as iso-C15 : 0 (41.4 %) and iso-C16 : 0 (24.7 %).
32375934	8	37	theme	dominant	979:986	arg1	acids					994:998	The dominant fatty acids	975:998	The dominant fatty acids	975:998	The dominant fatty acids were identified as iso-C15 : 0 (41.4 %) and iso-C16 : 0 (24.7 %).
32375934	8	37	theme	dominant	979:986	arg1	 0					1053:1054	 0	1053:1054	 0	1053:1054	The dominant fatty acids were identified as iso-C15 : 0 (41.4 %) and iso-C16 : 0 (24.7 %).
32375934	8	37	theme	dominant	979:986	arg1	iso-C16 					1044:1051	iso-C16 	1044:1051	iso-C16 	1044:1051	The dominant fatty acids were identified as iso-C15 : 0 (41.4 %) and iso-C16 : 0 (24.7 %).
32375934	8	37	theme	dominant	979:986	arg1	iso-C15 					1019:1026	iso-C15 	1019:1026	iso-C15 	1019:1026	The dominant fatty acids were identified as iso-C15 : 0 (41.4 %) and iso-C16 : 0 (24.7 %).
32375934	9	38	theme	JLT9T	1093:1097	arg1	lipids					1076:1081	The polar lipids	1066:1081	The polar lipids of strain JLT9T	1066:1097	The polar lipids of strain JLT9T comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, three unidentified glycolipid and an unidentified phospholipid.
32375934	9	39	theme	strain	1086:1091	arg1	JLT9T					1093:1097	strain JLT9T	1086:1097	strain JLT9T	1086:1097	The polar lipids of strain JLT9T comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, three unidentified glycolipid and an unidentified phospholipid.
32375934	14	40	theme	=CGMCC	1609:1614	arg1	31502T					1629:1634	=CGMCC 1.15779T=JCM 31502T	1609:1634	=CGMCC 1.15779T=JCM 31502T	1609:1634	The type strain is JLT9T (=CGMCC 1.15779T=JCM 31502T).
32375934	14	40	theme	=CGMCC	1609:1614	arg1	JLT9T					1602:1606	JLT9T	1602:1606	JLT9T (=CGMCC 1.15779T=JCM 31502T)	1602:1635	The type strain is JLT9T (=CGMCC 1.15779T=JCM 31502T).
32375934	14	41	theme	type	1587:1590	arg1	strain					1592:1597	The type strain	1583:1597	The type strain	1583:1597	The type strain is JLT9T (=CGMCC 1.15779T=JCM 31502T).
32375934	14	41	theme	type	1587:1590	arg1	JLT9T					1602:1606	JLT9T	1602:1606	JLT9T (=CGMCC 1.15779T=JCM 31502T)	1602:1635	The type strain is JLT9T (=CGMCC 1.15779T=JCM 31502T).
32375934	6	42	theme	strain	860:865	arg1	JLT9T					867:871	strain JLT9T	860:871	strain JLT9T	860:871	The digital DNA-DNA genome hybridization values between strain JLT9T and the closest related strain S. marinus DSM 15273T was 34.30 %.
32375934	12	43	theme	distinct	1410:1417	arg1	characteristics					1430:1444	several distinct phenotypic characteristics	1402:1444	several distinct phenotypic characteristics	1402:1444	On the basis of phylogenetic data and several distinct phenotypic characteristics, strain JLT9T represents a novel species of the genus Serinicoccus, for which the name Serinicoccus hydrothermalis sp.
32375934	2	44	dep	Kueishantao	264:274	arg1	Island					276:281	Island	276:281	Island	276:281	A Gram-stain-positive, non-flagellated, non-gliding, coccoid bacterial strain, designated JLT9T, was isolated from the shallow-sea hydrothermal system off Kueishantao Island, Taiwan, ROC.
32375934	2	44	dep	Kueishantao	264:274	arg1	off					260:262	off	260:262	off	260:262	A Gram-stain-positive, non-flagellated, non-gliding, coccoid bacterial strain, designated JLT9T, was isolated from the shallow-sea hydrothermal system off Kueishantao Island, Taiwan, ROC.
32375934	4	45	theme	Serinicoccus	490:501	arg1	15273T					515:520	Serinicoccus marinus DSM 15273T	490:520	Serinicoccus marinus DSM 15273T (98.83 %)	490:530	Strain JLT9T exhibited highest 16S rRNA gene sequence similarity to Serinicoccus marinus DSM 15273T (98.83 %).
32375934	4	45	theme	Serinicoccus	490:501	arg1	%					529:529	98.83 %	523:529	98.83 %	523:529	Strain JLT9T exhibited highest 16S rRNA gene sequence similarity to Serinicoccus marinus DSM 15273T (98.83 %).
32375934	12	46	theme	novel	1473:1477	arg1	species					1479:1485	a novel species	1471:1485	a novel species	1471:1485	On the basis of phylogenetic data and several distinct phenotypic characteristics, strain JLT9T represents a novel species of the genus Serinicoccus, for which the name Serinicoccus hydrothermalis sp.
32375934	4	47	theme	Strain	422:427	arg1	JLT9T					429:433	Strain JLT9T	422:433	Strain JLT9T	422:433	Strain JLT9T exhibited highest 16S rRNA gene sequence similarity to Serinicoccus marinus DSM 15273T (98.83 %).
32375934	3	48	dep	%	408:408	arg1	w/v					411:413	w/v	411:413	w/v	411:413	Strain JLT9T was aerobic, chemoheterotrophic and grew optimally at 35 °C, at pH 6.0 and in the presence of 2.5 % (w/v) NaCl.
32375934	3	49	theme	NaCl	416:419	arg1	presence					392:399	the presence	388:399	the presence of 2.5 % (w/v) NaCl	388:419	Strain JLT9T was aerobic, chemoheterotrophic and grew optimally at 35 °C, at pH 6.0 and in the presence of 2.5 % (w/v) NaCl.
32375934	11	50	theme	cell	1294:1297	arg1	wall					1299:1302	The cell wall	1290:1302	The cell wall	1290:1302	The cell wall contained ornithine and serine, and no diaminopimelic acid.
32375934	2	51	dep	Gram-stain-positive	111:129	arg1	non-flagellated					132:146	non-flagellated	132:146	non-flagellated	132:146	A Gram-stain-positive, non-flagellated, non-gliding, coccoid bacterial strain, designated JLT9T, was isolated from the shallow-sea hydrothermal system off Kueishantao Island, Taiwan, ROC.
32375934	2	51	dep	Gram-stain-positive	111:129	arg1	coccoid					162:168	coccoid	162:168	coccoid	162:168	A Gram-stain-positive, non-flagellated, non-gliding, coccoid bacterial strain, designated JLT9T, was isolated from the shallow-sea hydrothermal system off Kueishantao Island, Taiwan, ROC.
32375934	2	51	dep	Gram-stain-positive	111:129	arg1	non-gliding					149:159	non-gliding	149:159	non-gliding	149:159	A Gram-stain-positive, non-flagellated, non-gliding, coccoid bacterial strain, designated JLT9T, was isolated from the shallow-sea hydrothermal system off Kueishantao Island, Taiwan, ROC.
32375934	2	52	theme	shallow-sea	228:238	arg1	system					253:258	the shallow-sea hydrothermal system	224:258	the shallow-sea hydrothermal system	224:258	A Gram-stain-positive, non-flagellated, non-gliding, coccoid bacterial strain, designated JLT9T, was isolated from the shallow-sea hydrothermal system off Kueishantao Island, Taiwan, ROC.
32375934	12	53	theme	Serinicoccus	1533:1544	arg1	sp					1561:1562	the name Serinicoccus hydrothermalis sp	1524:1562	the name Serinicoccus hydrothermalis sp	1524:1562	On the basis of phylogenetic data and several distinct phenotypic characteristics, strain JLT9T represents a novel species of the genus Serinicoccus, for which the name Serinicoccus hydrothermalis sp.
32375934	2	54	theme	Gram-stain-positive	111:129	arg1	strain					180:185	A Gram-stain-positive, non-flagellated, non-gliding, coccoid bacterial strain	109:185	A Gram-stain-positive, non-flagellated, non-gliding, coccoid bacterial strain	109:185	A Gram-stain-positive, non-flagellated, non-gliding, coccoid bacterial strain, designated JLT9T, was isolated from the shallow-sea hydrothermal system off Kueishantao Island, Taiwan, ROC.
32375934	4	55	theme	DSM	511:513	arg1	15273T					515:520	Serinicoccus marinus DSM 15273T	490:520	Serinicoccus marinus DSM 15273T (98.83 %)	490:530	Strain JLT9T exhibited highest 16S rRNA gene sequence similarity to Serinicoccus marinus DSM 15273T (98.83 %).
32375934	4	55	theme	DSM	511:513	arg1	%					529:529	98.83 %	523:529	98.83 %	523:529	Strain JLT9T exhibited highest 16S rRNA gene sequence similarity to Serinicoccus marinus DSM 15273T (98.83 %).
32375934	5	56	theme	genus	628:632	arg1	Serinicoccus					634:645	the genus Serinicoccus	624:645	the genus Serinicoccus	624:645	Phylogenetic trees based on 16S rRNA gene sequences revealed that strain JLT9T belonged to the genus Serinicoccus, clustering with Serinicoccus marinus JC1078T, Serinicoccus profundi MCCC 1A05965T, Serinicoccus sediminis GP-T3-3T and Serinicoccus chungangensis CAU9536T.
32375934	6	57	theme	hybridization	831:843	arg1	%					936:936	34.30 %	930:936	34.30 %	930:936	The digital DNA-DNA genome hybridization values between strain JLT9T and the closest related strain S. marinus DSM 15273T was 34.30 %.
32375934	6	57	theme	hybridization	831:843	arg1	values					845:850	The digital DNA-DNA genome hybridization values	804:850	The digital DNA-DNA genome hybridization values between strain JLT9T and the closest related strain S. marinus DSM 15273T	804:924	The digital DNA-DNA genome hybridization values between strain JLT9T and the closest related strain S. marinus DSM 15273T was 34.30 %.
32375934	11	58	contain	contained	1304:1312	arg2	ornithine					1314:1322	ornithine	1314:1322	ornithine	1314:1322	The cell wall contained ornithine and serine, and no diaminopimelic acid.
32375934	11	58	contain	contained	1304:1312	arg1	wall					1299:1302	The cell wall	1290:1302	The cell wall	1290:1302	The cell wall contained ornithine and serine, and no diaminopimelic acid.
32375934	11	58	contain	contained	1304:1312	arg2	acid					1358:1361	no diaminopimelic acid	1340:1361	no diaminopimelic acid	1340:1361	The cell wall contained ornithine and serine, and no diaminopimelic acid.
32375934	11	58	contain	contained	1304:1312	arg2	serine					1328:1333	serine	1328:1333	serine	1328:1333	The cell wall contained ornithine and serine, and no diaminopimelic acid.
32375934	9	59	theme	unidentified	1182:1193	arg1	glycolipid					1195:1204	three unidentified glycolipid	1176:1204	three unidentified glycolipid	1176:1204	The polar lipids of strain JLT9T comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, three unidentified glycolipid and an unidentified phospholipid.
32375934	12	60	theme	strain	1447:1452	arg1	JLT9T					1454:1458	strain JLT9T	1447:1458	strain JLT9T	1447:1458	On the basis of phylogenetic data and several distinct phenotypic characteristics, strain JLT9T represents a novel species of the genus Serinicoccus, for which the name Serinicoccus hydrothermalis sp.
32375934	12	61	theme	name	1528:1531	arg1	sp					1561:1562	the name Serinicoccus hydrothermalis sp	1524:1562	the name Serinicoccus hydrothermalis sp	1524:1562	On the basis of phylogenetic data and several distinct phenotypic characteristics, strain JLT9T represents a novel species of the genus Serinicoccus, for which the name Serinicoccus hydrothermalis sp.
32375934	3	62	theme	Strain	297:302	arg1	JLT9T					304:308	Strain JLT9T	297:308	Strain JLT9T	297:308	Strain JLT9T was aerobic, chemoheterotrophic and grew optimally at 35 °C, at pH 6.0 and in the presence of 2.5 % (w/v) NaCl.
32375934	6	63	theme	genome	824:829	arg1	hybridization					831:843	digital DNA-DNA genome hybridization	808:843	The digital DNA-DNA genome hybridization values between strain JLT9T and the closest related strain S. marinus DSM 15273T	804:924	The digital DNA-DNA genome hybridization values between strain JLT9T and the closest related strain S. marinus DSM 15273T was 34.30 %.
32375934	2	64	theme	hydrothermal	240:251	arg1	system					253:258	the shallow-sea hydrothermal system	224:258	the shallow-sea hydrothermal system	224:258	A Gram-stain-positive, non-flagellated, non-gliding, coccoid bacterial strain, designated JLT9T, was isolated from the shallow-sea hydrothermal system off Kueishantao Island, Taiwan, ROC.
32375934	6	65	theme	S.	904:905	arg1	15273T					919:924	S. marinus DSM 15273T	904:924	S. marinus DSM 15273T	904:924	The digital DNA-DNA genome hybridization values between strain JLT9T and the closest related strain S. marinus DSM 15273T was 34.30 %.
32375934	12	66	theme	Serinicoccus	1500:1511	arg1	species					1479:1485	a novel species	1471:1485	a novel species	1471:1485	On the basis of phylogenetic data and several distinct phenotypic characteristics, strain JLT9T represents a novel species of the genus Serinicoccus, for which the name Serinicoccus hydrothermalis sp.
32375934	6	67	theme	DNA-DNA	816:822	arg1	hybridization					831:843	digital DNA-DNA genome hybridization	808:843	The digital DNA-DNA genome hybridization values between strain JLT9T and the closest related strain S. marinus DSM 15273T	804:924	The digital DNA-DNA genome hybridization values between strain JLT9T and the closest related strain S. marinus DSM 15273T was 34.30 %.
32375934	7	68	theme	72.43 mol	963:971	arg1	content					951:957	The DNA G+C content	939:957	The DNA G+C content	939:957	The DNA G+C content was 72.43 mol%.
32375934	7	68	theme	72.43 mol	963:971	arg1	%					972:972	72.43 mol%	963:972	72.43 mol%	963:972	The DNA G+C content was 72.43 mol%.
32375934	12	69	theme	genus	1494:1498	arg1	Serinicoccus					1500:1511	the genus Serinicoccus	1490:1511	the genus Serinicoccus	1490:1511	On the basis of phylogenetic data and several distinct phenotypic characteristics, strain JLT9T represents a novel species of the genus Serinicoccus, for which the name Serinicoccus hydrothermalis sp.
32375934	5	70	dep	Serinicoccus	664:675	arg1	1A05965T					721:728	1A05965T	721:728	1A05965T	721:728	Phylogenetic trees based on 16S rRNA gene sequences revealed that strain JLT9T belonged to the genus Serinicoccus, clustering with Serinicoccus marinus JC1078T, Serinicoccus profundi MCCC 1A05965T, Serinicoccus sediminis GP-T3-3T and Serinicoccus chungangensis CAU9536T.
32375934	5	70	dep	Serinicoccus	664:675	arg1	CAU9536T					794:801	CAU9536T	794:801	CAU9536T	794:801	Phylogenetic trees based on 16S rRNA gene sequences revealed that strain JLT9T belonged to the genus Serinicoccus, clustering with Serinicoccus marinus JC1078T, Serinicoccus profundi MCCC 1A05965T, Serinicoccus sediminis GP-T3-3T and Serinicoccus chungangensis CAU9536T.
32375934	5	70	dep	Serinicoccus	664:675	arg1	sediminis					744:752	sediminis	744:752	sediminis	744:752	Phylogenetic trees based on 16S rRNA gene sequences revealed that strain JLT9T belonged to the genus Serinicoccus, clustering with Serinicoccus marinus JC1078T, Serinicoccus profundi MCCC 1A05965T, Serinicoccus sediminis GP-T3-3T and Serinicoccus chungangensis CAU9536T.
32375934	5	70	dep	Serinicoccus	664:675	arg1	Serinicoccus					694:705	Serinicoccus	694:705	Serinicoccus	694:705	Phylogenetic trees based on 16S rRNA gene sequences revealed that strain JLT9T belonged to the genus Serinicoccus, clustering with Serinicoccus marinus JC1078T, Serinicoccus profundi MCCC 1A05965T, Serinicoccus sediminis GP-T3-3T and Serinicoccus chungangensis CAU9536T.
32375934	5	70	dep	Serinicoccus	664:675	arg1	marinus					677:683	Serinicoccus marinus JC1078T, Serinicoccus profundi MCCC 1A05965T, Serinicoccus sediminis GP-T3-3T and Serinicoccus chungangensis CAU9536T	664:801	Serinicoccus marinus JC1078T, Serinicoccus profundi MCCC 1A05965T, Serinicoccus sediminis GP-T3-3T and Serinicoccus chungangensis CAU9536T	664:801	Phylogenetic trees based on 16S rRNA gene sequences revealed that strain JLT9T belonged to the genus Serinicoccus, clustering with Serinicoccus marinus JC1078T, Serinicoccus profundi MCCC 1A05965T, Serinicoccus sediminis GP-T3-3T and Serinicoccus chungangensis CAU9536T.
32375934	6	71	theme	digital	808:814	arg1	hybridization					831:843	digital DNA-DNA genome hybridization	808:843	The digital DNA-DNA genome hybridization values between strain JLT9T and the closest related strain S. marinus DSM 15273T	804:924	The digital DNA-DNA genome hybridization values between strain JLT9T and the closest related strain S. marinus DSM 15273T was 34.30 %.
32375934	9	72	theme	polar	1070:1074	arg1	lipids					1076:1081	The polar lipids	1066:1081	The polar lipids of strain JLT9T	1066:1097	The polar lipids of strain JLT9T comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, three unidentified glycolipid and an unidentified phospholipid.
32375934	4	73	theme	gene	462:465	arg1	similarity					476:485	highest 16S rRNA gene sequence similarity	445:485	highest 16S rRNA gene sequence similarity to Serinicoccus marinus DSM 15273T (98.83 %)	445:530	Strain JLT9T exhibited highest 16S rRNA gene sequence similarity to Serinicoccus marinus DSM 15273T (98.83 %).
32375934	6	74	dep	JLT9T	867:871	arg1	15273T					919:924	S. marinus DSM 15273T	904:924	S. marinus DSM 15273T	904:924	The digital DNA-DNA genome hybridization values between strain JLT9T and the closest related strain S. marinus DSM 15273T was 34.30 %.
32213250	14	0	theme	=KCTC	1511:1515	arg1	9695T					1529:1533	=KCTC 43010T=CECT 9695T	1511:1533	=KCTC 43010T=CECT 9695T	1511:1533	The type strain is HMF5848T (=KCTC 43010T=CECT 9695T).
32213250	14	0	theme	=KCTC	1511:1515	arg1	HMF5848T					1501:1508	HMF5848T	1501:1508	HMF5848T (=KCTC 43010T=CECT 9695T)	1501:1534	The type strain is HMF5848T (=KCTC 43010T=CECT 9695T).
32213250	11	1	dep	in	1189:1190	arg1	silico					1192:1197	silico	1192:1197	silico	1192:1197	The values of in silico DNA-DNA hybridization and average nucleotide identity between strains HMF5848T and B. humi DSM 16318T were 25.8 and 69.7 %, respectively.
32213250	6	2	theme	only	601:604	arg1	quinone					618:624	The only respiratory quinone	597:624	The only respiratory quinone	597:624	The only respiratory quinone was menaquinone-7.
32213250	6	2	theme	only	601:604	arg1	menaquinone-7					630:642	menaquinone-7	630:642	menaquinone-7	630:642	The only respiratory quinone was menaquinone-7.
32213250	10	3	theme	Bacillus	1136:1143	arg1	%					1171:1171	96.0 %	1166:1171	96.0 %	1166:1171	Strains HMF5848T showed the highest 16S rRNA gene sequence similarities to Bacillus humi LMG 22167T (96.1 %), Bacillus isabeliae CVS-8T (96.0 %) and Bacillus luteolus YIM 93174T (96.0 %).
32213250	10	3	theme	Bacillus	1136:1143	arg1	93174T					1158:1163	Bacillus luteolus YIM 93174T	1136:1163	Bacillus luteolus YIM 93174T (96.0 %)	1136:1172	Strains HMF5848T showed the highest 16S rRNA gene sequence similarities to Bacillus humi LMG 22167T (96.1 %), Bacillus isabeliae CVS-8T (96.0 %) and Bacillus luteolus YIM 93174T (96.0 %).
32213250	9	4	theme	YIM	975:977	arg1	93174T					979:984	Bacillus luteolus YIM 93174T	957:984	Bacillus luteolus YIM 93174T	957:984	Phylogenetic trees based on 16S rRNA gene sequences showed that strains HMF5848T and HME7618 clustered with Bacillus luteolus YIM 93174T.
32213250	4	5	theme	fatty	476:480	arg1	acids					482:486	The major fatty acids	466:486	The major fatty acids	466:486	The major fatty acids were iso-C15 : 0, iso-C16 : 0 and anteiso-C15 : 0.
32213250	4	5	theme	fatty	476:480	arg1	iso-C15 					493:500	iso-C15 	493:500	iso-C15 	493:500	The major fatty acids were iso-C15 : 0, iso-C16 : 0 and anteiso-C15 : 0.
32213250	9	6	theme	Phylogenetic	849:860	arg1	trees					862:866	Phylogenetic trees	849:866	Phylogenetic trees based on 16S rRNA gene sequences	849:899	Phylogenetic trees based on 16S rRNA gene sequences showed that strains HMF5848T and HME7618 clustered with Bacillus luteolus YIM 93174T.
32213250	2	7	dep	Gram-stain-positive	79:97	arg1	aerobic					109:115	aerobic	109:115	aerobic	109:115	Two strains of Gram-stain-positive, strictly aerobic, motile, spore-forming, rod-shaped, moderately halotolerant bacteria, designated as HMF5848T and HME7618, were isolated from salt/brine and subjected to a polyphasic taxonomic investigation.
32213250	2	7	dep	Gram-stain-positive	79:97	arg1	rod-shaped					141:150	rod-shaped	141:150	rod-shaped	141:150	Two strains of Gram-stain-positive, strictly aerobic, motile, spore-forming, rod-shaped, moderately halotolerant bacteria, designated as HMF5848T and HME7618, were isolated from salt/brine and subjected to a polyphasic taxonomic investigation.
32213250	2	7	dep	Gram-stain-positive	79:97	arg1	halotolerant					164:175	halotolerant	164:175	halotolerant	164:175	Two strains of Gram-stain-positive, strictly aerobic, motile, spore-forming, rod-shaped, moderately halotolerant bacteria, designated as HMF5848T and HME7618, were isolated from salt/brine and subjected to a polyphasic taxonomic investigation.
32213250	2	7	dep	Gram-stain-positive	79:97	arg1	spore-forming					126:138	spore-forming	126:138	spore-forming	126:138	Two strains of Gram-stain-positive, strictly aerobic, motile, spore-forming, rod-shaped, moderately halotolerant bacteria, designated as HMF5848T and HME7618, were isolated from salt/brine and subjected to a polyphasic taxonomic investigation.
32213250	2	7	dep	Gram-stain-positive	79:97	arg1	motile					118:123	motile	118:123	motile	118:123	Two strains of Gram-stain-positive, strictly aerobic, motile, spore-forming, rod-shaped, moderately halotolerant bacteria, designated as HMF5848T and HME7618, were isolated from salt/brine and subjected to a polyphasic taxonomic investigation.
32213250	7	8	theme	unidentified	791:802	arg1	lipids					810:815	two unidentified polar lipids	787:815	two unidentified polar lipids	787:815	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, four unidentified glycolipids, three unidentified phospholipids and two unidentified polar lipids.
32213250	7	8	theme	unidentified	791:802	arg1	diphosphatidylglycerol					673:694	diphosphatidylglycerol	673:694	diphosphatidylglycerol	673:694	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, four unidentified glycolipids, three unidentified phospholipids and two unidentified polar lipids.
32213250	4	9	theme	major	470:474	arg1	acids					482:486	The major fatty acids	466:486	The major fatty acids	466:486	The major fatty acids were iso-C15 : 0, iso-C16 : 0 and anteiso-C15 : 0.
32213250	4	9	theme	major	470:474	arg1	iso-C15 					493:500	iso-C15 	493:500	iso-C15 	493:500	The major fatty acids were iso-C15 : 0, iso-C16 : 0 and anteiso-C15 : 0.
32213250	10	10	dep	Bacillus	1136:1143	arg1	luteolus					1145:1152	luteolus	1145:1152	luteolus	1145:1152	Strains HMF5848T showed the highest 16S rRNA gene sequence similarities to Bacillus humi LMG 22167T (96.1 %), Bacillus isabeliae CVS-8T (96.0 %) and Bacillus luteolus YIM 93174T (96.0 %).
32213250	1	11	theme	commercial	41:50	arg1	salt					58:61	commercial solar salt	41:61	commercial solar salt	41:61	nov., isolated from commercial solar salt.
32213250	7	12	theme	polar	804:808	arg1	lipids					810:815	two unidentified polar lipids	787:815	two unidentified polar lipids	787:815	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, four unidentified glycolipids, three unidentified phospholipids and two unidentified polar lipids.
32213250	7	12	theme	polar	804:808	arg1	diphosphatidylglycerol					673:694	diphosphatidylglycerol	673:694	diphosphatidylglycerol	673:694	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, four unidentified glycolipids, three unidentified phospholipids and two unidentified polar lipids.
32213250	12	13	theme	strain	1412:1417	arg1	HMF5848T					1419:1426	strain HMF5848T	1412:1426	strain HMF5848T	1412:1426	On the basis of phylogenetic, physiological and chemotaxonomic properties, strain HMF5848T represents a novel species, Bacillus salinus sp.
32213250	10	14	theme	gene	1032:1035	arg1	similarities					1046:1057	the highest 16S rRNA gene sequence similarities	1011:1057	the highest 16S rRNA gene sequence similarities to Bacillus humi LMG 22167T (96.1 %), Bacillus isabeliae CVS-8T (96.0 %) and Bacillus luteolus YIM 93174T (96.0 %)	1011:1172	Strains HMF5848T showed the highest 16S rRNA gene sequence similarities to Bacillus humi LMG 22167T (96.1 %), Bacillus isabeliae CVS-8T (96.0 %) and Bacillus luteolus YIM 93174T (96.0 %).
32213250	1	15	theme	solar	52:56	arg1	salt					58:61	commercial solar salt	41:61	commercial solar salt	41:61	nov., isolated from commercial solar salt.
32213250	3	16	dep	optimum	422:428	arg1	37 °C					431:435	37 °C	431:435	37 °C	431:435	Growth of both yellow-coloured strains occurred in the presence of 1-9 % NaCl (w/v; optimum, 2-3 %), at 15-45 °C (optimum, 37 °C) and pH 6-9 (optimum, pH 7).
32213250	9	17	dep	strains	913:919	arg1	strains					913:919	strains HMF5848T and HME7618	913:940	strains HMF5848T and HME7618	913:940	Phylogenetic trees based on 16S rRNA gene sequences showed that strains HMF5848T and HME7618 clustered with Bacillus luteolus YIM 93174T.
32213250	9	17	dep	strains	913:919	arg1	HME7618					934:940	HME7618	934:940	HME7618	934:940	Phylogenetic trees based on 16S rRNA gene sequences showed that strains HMF5848T and HME7618 clustered with Bacillus luteolus YIM 93174T.
32213250	9	17	dep	strains	913:919	arg1	HMF5848T					921:928	HMF5848T	921:928	HMF5848T	921:928	Phylogenetic trees based on 16S rRNA gene sequences showed that strains HMF5848T and HME7618 clustered with Bacillus luteolus YIM 93174T.
32213250	10	18	theme	Bacillus	1097:1104	arg1	CVS-8T					1116:1121	Bacillus isabeliae CVS-8T	1097:1121	Bacillus isabeliae CVS-8T (96.0 %)	1097:1130	Strains HMF5848T showed the highest 16S rRNA gene sequence similarities to Bacillus humi LMG 22167T (96.1 %), Bacillus isabeliae CVS-8T (96.0 %) and Bacillus luteolus YIM 93174T (96.0 %).
32213250	10	18	theme	Bacillus	1097:1104	arg1	%					1129:1129	96.0 %	1124:1129	96.0 %	1124:1129	Strains HMF5848T showed the highest 16S rRNA gene sequence similarities to Bacillus humi LMG 22167T (96.1 %), Bacillus isabeliae CVS-8T (96.0 %) and Bacillus luteolus YIM 93174T (96.0 %).
32213250	3	19	theme	strains	339:345	arg1	Growth					308:313	Growth	308:313	Growth of both yellow-coloured strains	308:345	Growth of both yellow-coloured strains occurred in the presence of 1-9 % NaCl (w/v; optimum, 2-3 %), at 15-45 °C (optimum, 37 °C) and pH 6-9 (optimum, pH 7).
32213250	10	20	theme	rRNA	1027:1030	arg1	similarities					1046:1057	the highest 16S rRNA gene sequence similarities	1011:1057	the highest 16S rRNA gene sequence similarities to Bacillus humi LMG 22167T (96.1 %), Bacillus isabeliae CVS-8T (96.0 %) and Bacillus luteolus YIM 93174T (96.0 %)	1011:1172	Strains HMF5848T showed the highest 16S rRNA gene sequence similarities to Bacillus humi LMG 22167T (96.1 %), Bacillus isabeliae CVS-8T (96.0 %) and Bacillus luteolus YIM 93174T (96.0 %).
32213250	3	21	dep	 NaCl	380:384	arg1	%					405:405	2-3 %	401:405	2-3 %	401:405	Growth of both yellow-coloured strains occurred in the presence of 1-9 % NaCl (w/v; optimum, 2-3 %), at 15-45 °C (optimum, 37 °C) and pH 6-9 (optimum, pH 7).
32213250	3	21	dep	 NaCl	380:384	arg1	w/v					387:389	w/v	387:389	w/v	387:389	Growth of both yellow-coloured strains occurred in the presence of 1-9 % NaCl (w/v; optimum, 2-3 %), at 15-45 °C (optimum, 37 °C) and pH 6-9 (optimum, pH 7).
32213250	10	22	theme	YIM	1154:1156	arg1	%					1171:1171	96.0 %	1166:1171	96.0 %	1166:1171	Strains HMF5848T showed the highest 16S rRNA gene sequence similarities to Bacillus humi LMG 22167T (96.1 %), Bacillus isabeliae CVS-8T (96.0 %) and Bacillus luteolus YIM 93174T (96.0 %).
32213250	10	22	theme	YIM	1154:1156	arg1	93174T					1158:1163	Bacillus luteolus YIM 93174T	1136:1163	Bacillus luteolus YIM 93174T (96.0 %)	1136:1172	Strains HMF5848T showed the highest 16S rRNA gene sequence similarities to Bacillus humi LMG 22167T (96.1 %), Bacillus isabeliae CVS-8T (96.0 %) and Bacillus luteolus YIM 93174T (96.0 %).
32213250	11	23	theme	average	1225:1231	arg1	identity					1244:1251	average nucleotide identity	1225:1251	average nucleotide identity between strains HMF5848T and B. humi DSM 16318T	1225:1299	The values of in silico DNA-DNA hybridization and average nucleotide identity between strains HMF5848T and B. humi DSM 16318T were 25.8 and 69.7 %, respectively.
32213250	9	24	theme	16S	877:879	arg1	sequences					891:899	16S rRNA gene sequences	877:899	16S rRNA gene sequences	877:899	Phylogenetic trees based on 16S rRNA gene sequences showed that strains HMF5848T and HME7618 clustered with Bacillus luteolus YIM 93174T.
32213250	10	25	theme	16S	1023:1025	arg1	similarities					1046:1057	the highest 16S rRNA gene sequence similarities	1011:1057	the highest 16S rRNA gene sequence similarities to Bacillus humi LMG 22167T (96.1 %), Bacillus isabeliae CVS-8T (96.0 %) and Bacillus luteolus YIM 93174T (96.0 %)	1011:1172	Strains HMF5848T showed the highest 16S rRNA gene sequence similarities to Bacillus humi LMG 22167T (96.1 %), Bacillus isabeliae CVS-8T (96.0 %) and Bacillus luteolus YIM 93174T (96.0 %).
32213250	0	26	theme	Bacillus	0:7	arg1	sp					17:18	Bacillus salinus sp	0:18	Bacillus salinus sp.	0:19	Bacillus salinus sp.
32213250	7	27	theme	unidentified	756:767	arg1	phospholipids					769:781	three unidentified phospholipids	750:781	three unidentified phospholipids	750:781	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, four unidentified glycolipids, three unidentified phospholipids and two unidentified polar lipids.
32213250	7	27	theme	unidentified	756:767	arg1	diphosphatidylglycerol					673:694	diphosphatidylglycerol	673:694	diphosphatidylglycerol	673:694	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, four unidentified glycolipids, three unidentified phospholipids and two unidentified polar lipids.
32213250	10	28	theme	LMG	1076:1078	arg1	22167T					1080:1085	Bacillus humi LMG 22167T	1062:1085	Bacillus humi LMG 22167T (96.1 %)	1062:1094	Strains HMF5848T showed the highest 16S rRNA gene sequence similarities to Bacillus humi LMG 22167T (96.1 %), Bacillus isabeliae CVS-8T (96.0 %) and Bacillus luteolus YIM 93174T (96.0 %).
32213250	10	28	theme	LMG	1076:1078	arg1	%					1093:1093	96.1 %	1088:1093	96.1 %	1088:1093	Strains HMF5848T showed the highest 16S rRNA gene sequence similarities to Bacillus humi LMG 22167T (96.1 %), Bacillus isabeliae CVS-8T (96.0 %) and Bacillus luteolus YIM 93174T (96.0 %).
32213250	0	29	theme	salinus	9:15	arg1	sp					17:18	Bacillus salinus sp	0:18	Bacillus salinus sp.	0:19	Bacillus salinus sp.
32213250	10	30	theme	highest	1015:1021	arg1	similarities					1046:1057	the highest 16S rRNA gene sequence similarities	1011:1057	the highest 16S rRNA gene sequence similarities to Bacillus humi LMG 22167T (96.1 %), Bacillus isabeliae CVS-8T (96.0 %) and Bacillus luteolus YIM 93174T (96.0 %)	1011:1172	Strains HMF5848T showed the highest 16S rRNA gene sequence similarities to Bacillus humi LMG 22167T (96.1 %), Bacillus isabeliae CVS-8T (96.0 %) and Bacillus luteolus YIM 93174T (96.0 %).
32213250	3	31	dep	w/v	387:389	arg1	optimum					392:398	optimum	392:398	optimum	392:398	Growth of both yellow-coloured strains occurred in the presence of 1-9 % NaCl (w/v; optimum, 2-3 %), at 15-45 °C (optimum, 37 °C) and pH 6-9 (optimum, pH 7).
32213250	3	32	theme	%	379:379	arg1	 NaCl					380:384	1-9 % NaCl	375:384	1-9 % NaCl (w/v; optimum, 2-3 %)	375:406	Growth of both yellow-coloured strains occurred in the presence of 1-9 % NaCl (w/v; optimum, 2-3 %), at 15-45 °C (optimum, 37 °C) and pH 6-9 (optimum, pH 7).
32213250	10	33	theme	Bacillus	1062:1069	arg1	22167T					1080:1085	Bacillus humi LMG 22167T	1062:1085	Bacillus humi LMG 22167T (96.1 %)	1062:1094	Strains HMF5848T showed the highest 16S rRNA gene sequence similarities to Bacillus humi LMG 22167T (96.1 %), Bacillus isabeliae CVS-8T (96.0 %) and Bacillus luteolus YIM 93174T (96.0 %).
32213250	10	33	theme	Bacillus	1062:1069	arg1	%					1093:1093	96.1 %	1088:1093	96.1 %	1088:1093	Strains HMF5848T showed the highest 16S rRNA gene sequence similarities to Bacillus humi LMG 22167T (96.1 %), Bacillus isabeliae CVS-8T (96.0 %) and Bacillus luteolus YIM 93174T (96.0 %).
32213250	2	34	theme	bacteria	177:184	arg1	strains					68:74	Two strains	64:74	Two strains	64:74	Two strains of Gram-stain-positive, strictly aerobic, motile, spore-forming, rod-shaped, moderately halotolerant bacteria, designated as HMF5848T and HME7618, were isolated from salt/brine and subjected to a polyphasic taxonomic investigation.
32213250	11	35	theme	hybridization	1207:1219	arg1	values					1179:1184	The values	1175:1184	The values of in silico DNA-DNA hybridization and average nucleotide identity between strains HMF5848T and B. humi DSM 16318T	1175:1299	The values of in silico DNA-DNA hybridization and average nucleotide identity between strains HMF5848T and B. humi DSM 16318T were 25.8 and 69.7 %, respectively.
32213250	11	35	theme	hybridization	1207:1219	arg1	%					1320:1320	25.8 and 69.7 %	1306:1320	25.8 and 69.7 %	1306:1320	The values of in silico DNA-DNA hybridization and average nucleotide identity between strains HMF5848T and B. humi DSM 16318T were 25.8 and 69.7 %, respectively.
32213250	8	36	theme	G+C	822:824	arg1	content					826:832	DNA G+C content	818:832	DNA G+C content	818:832	DNA G+C content was 37.4 mol%.
32213250	12	37	theme	phylogenetic	1353:1364	arg1	properties					1400:1409	phylogenetic, physiological and chemotaxonomic properties	1353:1409	phylogenetic, physiological and chemotaxonomic properties	1353:1409	On the basis of phylogenetic, physiological and chemotaxonomic properties, strain HMF5848T represents a novel species, Bacillus salinus sp.
32213250	11	38	theme	in	1189:1190	arg1	hybridization					1207:1219	in silico DNA-DNA hybridization	1189:1219	in silico DNA-DNA hybridization	1189:1219	The values of in silico DNA-DNA hybridization and average nucleotide identity between strains HMF5848T and B. humi DSM 16318T were 25.8 and 69.7 %, respectively.
32213250	5	39	theme	meso-diaminopimelic	571:589	arg1	peptidoglycan					553:565	The cell-wall peptidoglycan	539:565	The cell-wall peptidoglycan	539:565	The cell-wall peptidoglycan was meso-diaminopimelic acid.
32213250	5	39	theme	meso-diaminopimelic	571:589	arg1	acid					591:594	meso-diaminopimelic acid	571:594	meso-diaminopimelic acid	571:594	The cell-wall peptidoglycan was meso-diaminopimelic acid.
32213250	2	40	theme	taxonomic	283:291	arg1	investigation					293:305	a polyphasic taxonomic investigation	270:305	a polyphasic taxonomic investigation	270:305	Two strains of Gram-stain-positive, strictly aerobic, motile, spore-forming, rod-shaped, moderately halotolerant bacteria, designated as HMF5848T and HME7618, were isolated from salt/brine and subjected to a polyphasic taxonomic investigation.
32213250	8	41	theme	DNA	818:820	arg1	content					826:832	DNA G+C content	818:832	DNA G+C content	818:832	DNA G+C content was 37.4 mol%.
32213250	11	42	dep	strains	1261:1267	arg1	HMF5848T					1269:1276	HMF5848T	1269:1276	HMF5848T	1269:1276	The values of in silico DNA-DNA hybridization and average nucleotide identity between strains HMF5848T and B. humi DSM 16318T were 25.8 and 69.7 %, respectively.
32213250	11	42	dep	strains	1261:1267	arg1	strains					1261:1267	strains HMF5848T and B. humi DSM 16318T	1261:1299	strains HMF5848T and B. humi DSM 16318T	1261:1299	The values of in silico DNA-DNA hybridization and average nucleotide identity between strains HMF5848T and B. humi DSM 16318T were 25.8 and 69.7 %, respectively.
32213250	11	42	dep	strains	1261:1267	arg1	16318T					1294:1299	16318T	1294:1299	16318T	1294:1299	The values of in silico DNA-DNA hybridization and average nucleotide identity between strains HMF5848T and B. humi DSM 16318T were 25.8 and 69.7 %, respectively.
32213250	2	43	theme	polyphasic	272:281	arg1	investigation					293:305	a polyphasic taxonomic investigation	270:305	a polyphasic taxonomic investigation	270:305	Two strains of Gram-stain-positive, strictly aerobic, motile, spore-forming, rod-shaped, moderately halotolerant bacteria, designated as HMF5848T and HME7618, were isolated from salt/brine and subjected to a polyphasic taxonomic investigation.
32213250	7	44	theme	unidentified	724:735	arg1	diphosphatidylglycerol					673:694	diphosphatidylglycerol	673:694	diphosphatidylglycerol	673:694	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, four unidentified glycolipids, three unidentified phospholipids and two unidentified polar lipids.
32213250	7	44	theme	unidentified	724:735	arg1	glycolipids					737:747	four unidentified glycolipids	719:747	four unidentified glycolipids	719:747	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, four unidentified glycolipids, three unidentified phospholipids and two unidentified polar lipids.
32213250	11	45	theme	nucleotide	1233:1242	arg1	identity					1244:1251	average nucleotide identity	1225:1251	average nucleotide identity between strains HMF5848T and B. humi DSM 16318T	1225:1299	The values of in silico DNA-DNA hybridization and average nucleotide identity between strains HMF5848T and B. humi DSM 16318T were 25.8 and 69.7 %, respectively.
32213250	10	46	theme	sequence	1037:1044	arg1	similarities					1046:1057	the highest 16S rRNA gene sequence similarities	1011:1057	the highest 16S rRNA gene sequence similarities to Bacillus humi LMG 22167T (96.1 %), Bacillus isabeliae CVS-8T (96.0 %) and Bacillus luteolus YIM 93174T (96.0 %)	1011:1172	Strains HMF5848T showed the highest 16S rRNA gene sequence similarities to Bacillus humi LMG 22167T (96.1 %), Bacillus isabeliae CVS-8T (96.0 %) and Bacillus luteolus YIM 93174T (96.0 %).
32213250	10	47	dep	Bacillus	1097:1104	arg1	isabeliae					1106:1114	isabeliae	1106:1114	isabeliae	1106:1114	Strains HMF5848T showed the highest 16S rRNA gene sequence similarities to Bacillus humi LMG 22167T (96.1 %), Bacillus isabeliae CVS-8T (96.0 %) and Bacillus luteolus YIM 93174T (96.0 %).
32213250	11	48	theme	identity	1244:1251	arg1	values					1179:1184	The values	1175:1184	The values of in silico DNA-DNA hybridization and average nucleotide identity between strains HMF5848T and B. humi DSM 16318T	1175:1299	The values of in silico DNA-DNA hybridization and average nucleotide identity between strains HMF5848T and B. humi DSM 16318T were 25.8 and 69.7 %, respectively.
32213250	11	48	theme	identity	1244:1251	arg1	%					1320:1320	25.8 and 69.7 %	1306:1320	25.8 and 69.7 %	1306:1320	The values of in silico DNA-DNA hybridization and average nucleotide identity between strains HMF5848T and B. humi DSM 16318T were 25.8 and 69.7 %, respectively.
32213250	2	49	theme	Gram-stain-positive	79:97	arg1	bacteria					177:184	Gram-stain-positive, strictly aerobic, motile, spore-forming, rod-shaped, moderately halotolerant bacteria	79:184	Gram-stain-positive, strictly aerobic, motile, spore-forming, rod-shaped, moderately halotolerant bacteria	79:184	Two strains of Gram-stain-positive, strictly aerobic, motile, spore-forming, rod-shaped, moderately halotolerant bacteria, designated as HMF5848T and HME7618, were isolated from salt/brine and subjected to a polyphasic taxonomic investigation.
32213250	12	50	theme	chemotaxonomic	1385:1398	arg1	properties					1400:1409	phylogenetic, physiological and chemotaxonomic properties	1353:1409	phylogenetic, physiological and chemotaxonomic properties	1353:1409	On the basis of phylogenetic, physiological and chemotaxonomic properties, strain HMF5848T represents a novel species, Bacillus salinus sp.
32213250	12	51	dep	properties	1400:1409	arg1	basis					1344:1348	basis	1344:1348	basis	1344:1348	On the basis of phylogenetic, physiological and chemotaxonomic properties, strain HMF5848T represents a novel species, Bacillus salinus sp.
32213250	12	51	dep	properties	1400:1409	arg1	the					1340:1342	the	1340:1342	the	1340:1342	On the basis of phylogenetic, physiological and chemotaxonomic properties, strain HMF5848T represents a novel species, Bacillus salinus sp.
32213250	12	52	theme	Bacillus	1456:1463	arg1	species					1447:1453	a novel species	1439:1453	a novel species	1439:1453	On the basis of phylogenetic, physiological and chemotaxonomic properties, strain HMF5848T represents a novel species, Bacillus salinus sp.
32213250	12	52	theme	Bacillus	1456:1463	arg1	sp					1473:1474	Bacillus salinus sp	1456:1474	Bacillus salinus sp	1456:1474	On the basis of phylogenetic, physiological and chemotaxonomic properties, strain HMF5848T represents a novel species, Bacillus salinus sp.
32213250	3	53	theme	 NaCl	380:384	arg1	presence					363:370	the presence	359:370	the presence of 1-9 % NaCl (w/v; optimum, 2-3 %)	359:406	Growth of both yellow-coloured strains occurred in the presence of 1-9 % NaCl (w/v; optimum, 2-3 %), at 15-45 °C (optimum, 37 °C) and pH 6-9 (optimum, pH 7).
32213250	12	54	theme	physiological	1367:1379	arg1	properties					1400:1409	phylogenetic, physiological and chemotaxonomic properties	1353:1409	phylogenetic, physiological and chemotaxonomic properties	1353:1409	On the basis of phylogenetic, physiological and chemotaxonomic properties, strain HMF5848T represents a novel species, Bacillus salinus sp.
32213250	8	55	theme	37.4 mol	838:845	arg1	%					846:846	37.4 mol%	838:846	37.4 mol%	838:846	DNA G+C content was 37.4 mol%.
32213250	12	56	theme	salinus	1465:1471	arg1	species					1447:1453	a novel species	1439:1453	a novel species	1439:1453	On the basis of phylogenetic, physiological and chemotaxonomic properties, strain HMF5848T represents a novel species, Bacillus salinus sp.
32213250	12	56	theme	salinus	1465:1471	arg1	sp					1473:1474	Bacillus salinus sp	1456:1474	Bacillus salinus sp	1456:1474	On the basis of phylogenetic, physiological and chemotaxonomic properties, strain HMF5848T represents a novel species, Bacillus salinus sp.
32213250	9	57	theme	rRNA	881:884	arg1	sequences					891:899	16S rRNA gene sequences	877:899	16S rRNA gene sequences	877:899	Phylogenetic trees based on 16S rRNA gene sequences showed that strains HMF5848T and HME7618 clustered with Bacillus luteolus YIM 93174T.
32213250	7	58	theme	polar	655:659	arg1	diphosphatidylglycerol					673:694	diphosphatidylglycerol	673:694	diphosphatidylglycerol	673:694	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, four unidentified glycolipids, three unidentified phospholipids and two unidentified polar lipids.
32213250	7	58	theme	polar	655:659	arg1	lipids					661:666	The major polar lipids	645:666	The major polar lipids	645:666	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, four unidentified glycolipids, three unidentified phospholipids and two unidentified polar lipids.
32213250	5	59	theme	cell-wall	543:551	arg1	peptidoglycan					553:565	The cell-wall peptidoglycan	539:565	The cell-wall peptidoglycan	539:565	The cell-wall peptidoglycan was meso-diaminopimelic acid.
32213250	5	59	theme	cell-wall	543:551	arg1	acid					591:594	meso-diaminopimelic acid	571:594	meso-diaminopimelic acid	571:594	The cell-wall peptidoglycan was meso-diaminopimelic acid.
32213250	3	60	dep	optimum	450:456	arg1	pH					459:460	pH 7	459:462	pH 7	459:462	Growth of both yellow-coloured strains occurred in the presence of 1-9 % NaCl (w/v; optimum, 2-3 %), at 15-45 °C (optimum, 37 °C) and pH 6-9 (optimum, pH 7).
32213250	14	61	theme	type	1486:1489	arg1	strain					1491:1496	The type strain	1482:1496	The type strain	1482:1496	The type strain is HMF5848T (=KCTC 43010T=CECT 9695T).
32213250	14	61	theme	type	1486:1489	arg1	HMF5848T					1501:1508	HMF5848T	1501:1508	HMF5848T (=KCTC 43010T=CECT 9695T)	1501:1534	The type strain is HMF5848T (=KCTC 43010T=CECT 9695T).
32213250	10	62	theme	humi	1071:1074	arg1	22167T					1080:1085	Bacillus humi LMG 22167T	1062:1085	Bacillus humi LMG 22167T (96.1 %)	1062:1094	Strains HMF5848T showed the highest 16S rRNA gene sequence similarities to Bacillus humi LMG 22167T (96.1 %), Bacillus isabeliae CVS-8T (96.0 %) and Bacillus luteolus YIM 93174T (96.0 %).
32213250	10	62	theme	humi	1071:1074	arg1	%					1093:1093	96.1 %	1088:1093	96.1 %	1088:1093	Strains HMF5848T showed the highest 16S rRNA gene sequence similarities to Bacillus humi LMG 22167T (96.1 %), Bacillus isabeliae CVS-8T (96.0 %) and Bacillus luteolus YIM 93174T (96.0 %).
32213250	11	63	theme	DNA-DNA	1199:1205	arg1	hybridization					1207:1219	in silico DNA-DNA hybridization	1189:1219	in silico DNA-DNA hybridization	1189:1219	The values of in silico DNA-DNA hybridization and average nucleotide identity between strains HMF5848T and B. humi DSM 16318T were 25.8 and 69.7 %, respectively.
32213250	7	64	theme	major	649:653	arg1	diphosphatidylglycerol					673:694	diphosphatidylglycerol	673:694	diphosphatidylglycerol	673:694	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, four unidentified glycolipids, three unidentified phospholipids and two unidentified polar lipids.
32213250	7	64	theme	major	649:653	arg1	lipids					661:666	The major polar lipids	645:666	The major polar lipids	645:666	The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, four unidentified glycolipids, three unidentified phospholipids and two unidentified polar lipids.
32213250	4	65	dep	iso-C15 	493:500	arg1	 0					515:516	 0	515:516	 0	515:516	The major fatty acids were iso-C15 : 0, iso-C16 : 0 and anteiso-C15 : 0.
32213250	4	65	dep	iso-C15 	493:500	arg1	 0					502:503	 0	502:503	 0	502:503	The major fatty acids were iso-C15 : 0, iso-C16 : 0 and anteiso-C15 : 0.
32213250	4	65	dep	iso-C15 	493:500	arg1	 0					535:536	 0	535:536	 0	535:536	The major fatty acids were iso-C15 : 0, iso-C16 : 0 and anteiso-C15 : 0.
32213250	4	65	dep	iso-C15 	493:500	arg1	anteiso-C15 					522:533	anteiso-C15 	522:533	anteiso-C15 	522:533	The major fatty acids were iso-C15 : 0, iso-C16 : 0 and anteiso-C15 : 0.
32213250	9	66	theme	gene	886:889	arg1	sequences					891:899	16S rRNA gene sequences	877:899	16S rRNA gene sequences	877:899	Phylogenetic trees based on 16S rRNA gene sequences showed that strains HMF5848T and HME7618 clustered with Bacillus luteolus YIM 93174T.
32213250	9	67	theme	Bacillus	957:964	arg1	93174T					979:984	Bacillus luteolus YIM 93174T	957:984	Bacillus luteolus YIM 93174T	957:984	Phylogenetic trees based on 16S rRNA gene sequences showed that strains HMF5848T and HME7618 clustered with Bacillus luteolus YIM 93174T.
32213250	12	68	theme	novel	1441:1445	arg1	species					1447:1453	a novel species	1439:1453	a novel species	1439:1453	On the basis of phylogenetic, physiological and chemotaxonomic properties, strain HMF5848T represents a novel species, Bacillus salinus sp.
32213250	12	68	theme	novel	1441:1445	arg1	sp					1473:1474	Bacillus salinus sp	1456:1474	Bacillus salinus sp	1456:1474	On the basis of phylogenetic, physiological and chemotaxonomic properties, strain HMF5848T represents a novel species, Bacillus salinus sp.
32213250	3	69	theme	yellow-coloured	323:337	arg1	strains					339:345	both yellow-coloured strains	318:345	both yellow-coloured strains	318:345	Growth of both yellow-coloured strains occurred in the presence of 1-9 % NaCl (w/v; optimum, 2-3 %), at 15-45 °C (optimum, 37 °C) and pH 6-9 (optimum, pH 7).
32213250	14	70	theme	43010T=CECT	1517:1527	arg1	9695T					1529:1533	=KCTC 43010T=CECT 9695T	1511:1533	=KCTC 43010T=CECT 9695T	1511:1533	The type strain is HMF5848T (=KCTC 43010T=CECT 9695T).
32213250	14	70	theme	43010T=CECT	1517:1527	arg1	HMF5848T					1501:1508	HMF5848T	1501:1508	HMF5848T (=KCTC 43010T=CECT 9695T)	1501:1534	The type strain is HMF5848T (=KCTC 43010T=CECT 9695T).
32213250	6	71	theme	respiratory	606:616	arg1	quinone					618:624	The only respiratory quinone	597:624	The only respiratory quinone	597:624	The only respiratory quinone was menaquinone-7.
32213250	6	71	theme	respiratory	606:616	arg1	menaquinone-7					630:642	menaquinone-7	630:642	menaquinone-7	630:642	The only respiratory quinone was menaquinone-7.
32213250	3	72	theme	1-9 	375:378	arg1	 NaCl					380:384	1-9 % NaCl	375:384	1-9 % NaCl (w/v; optimum, 2-3 %)	375:406	Growth of both yellow-coloured strains occurred in the presence of 1-9 % NaCl (w/v; optimum, 2-3 %), at 15-45 °C (optimum, 37 °C) and pH 6-9 (optimum, pH 7).
32213250	9	73	theme	luteolus	966:973	arg1	93174T					979:984	Bacillus luteolus YIM 93174T	957:984	Bacillus luteolus YIM 93174T	957:984	Phylogenetic trees based on 16S rRNA gene sequences showed that strains HMF5848T and HME7618 clustered with Bacillus luteolus YIM 93174T.
32213250	2	74	attach	isolated	228:235	arg2	strains					68:74	Two strains	64:74	Two strains	64:74	Two strains of Gram-stain-positive, strictly aerobic, motile, spore-forming, rod-shaped, moderately halotolerant bacteria, designated as HMF5848T and HME7618, were isolated from salt/brine and subjected to a polyphasic taxonomic investigation.
32213250	2	74	attach	isolated	228:235	arg1	salt/brine					242:251	salt/brine	242:251	salt/brine	242:251	Two strains of Gram-stain-positive, strictly aerobic, motile, spore-forming, rod-shaped, moderately halotolerant bacteria, designated as HMF5848T and HME7618, were isolated from salt/brine and subjected to a polyphasic taxonomic investigation.
34576834	7	0	theme	several	1438:1444	arg1	species					1462:1468	several Bifidobacterium species	1438:1468	several Bifidobacterium species	1438:1468	Acetate correlated with several Bifidobacterium species.
34576834	8	1	theme	<	1593:1593	arg1	0.007					1595:1599	p < 0.007	1591:1599	p < 0.007	1591:1599	By univariate analysis, infants experiencing no bronchitis or LRTI, compared with those who did, showed higher acetate (p < 0.007) and B. longum subsp.
34576834	8	1	theme	<	1593:1593	arg1	acetate					1582:1588	higher acetate	1575:1588	higher acetate (p < 0.007)	1575:1600	By univariate analysis, infants experiencing no bronchitis or LRTI, compared with those who did, showed higher acetate (p < 0.007) and B. longum subsp.
34576834	2	2	dep	fucosyllactose	335:348	arg1	feeding					396:402	formula feeding	388:402	formula feeding	388:402	Exploratory findings of a randomized placebo-controlled trial associated 2'fucosyllactose (2'FL) and lacto-N-neotetraose (LNnT) formula feeding with reduced risk for reported bronchitis and lower respiratory tract illnesses (LRTI), as well as changes in gut microbiota composition.
34576834	12	3	theme	epithelial	1861:1870	arg1	protection					1880:1889	epithelial barrier protection	1861:1889	epithelial barrier protection	1861:1889	Metabolites in spent culture media, primarily due to acetate, supported epithelial barrier protection.
34576834	2	4	theme	microbiota	518:527	arg1	composition					529:539	gut microbiota composition	514:539	gut microbiota composition	514:539	Exploratory findings of a randomized placebo-controlled trial associated 2'fucosyllactose (2'FL) and lacto-N-neotetraose (LNnT) formula feeding with reduced risk for reported bronchitis and lower respiratory tract illnesses (LRTI), as well as changes in gut microbiota composition.
34576834	10	5	dep	lacto-N-tetraose	1697:1712	arg1	LNT					1715:1717	LNT	1715:1717	LNT	1715:1717	In vitro experiments demonstrate that 2'FL, LNnT and lacto-N-tetraose (LNT) stimulated B. longum subsp.
34576834	2	6	theme	placebo-controlled	297:314	arg1	trial					316:320	a randomized placebo-controlled trial	284:320	a randomized placebo-controlled trial	284:320	Exploratory findings of a randomized placebo-controlled trial associated 2'fucosyllactose (2'FL) and lacto-N-neotetraose (LNnT) formula feeding with reduced risk for reported bronchitis and lower respiratory tract illnesses (LRTI), as well as changes in gut microbiota composition.
34576834	2	7	from	illnesses	474:482	arg1	composition					529:539	gut microbiota composition	514:539	gut microbiota composition	514:539	Exploratory findings of a randomized placebo-controlled trial associated 2'fucosyllactose (2'FL) and lacto-N-neotetraose (LNnT) formula feeding with reduced risk for reported bronchitis and lower respiratory tract illnesses (LRTI), as well as changes in gut microbiota composition.
34576834	13	8	theme	reduced	2051:2057	arg1	risk					2059:2062	reduced risk	2051:2062	reduced risk for bronchitis and LRTI	2051:2086	(4) Conclusions: An early-life gut ecology characterized by Bifidobacterium-species-driven metabolic changes partly explains the observed clinical outcomes of reduced risk for bronchitis and LRTI in infants fed a formula with HMOs.
34576834	3	9	theme	gut	573:575	arg1	mechanisms					587:596	putative gut microbial mechanisms	564:596	putative gut microbial mechanisms linked with these clinical observations	564:636	We sought to identify putative gut microbial mechanisms linked with these clinical observations.
34576834	4	10	theme	reported	889:896	arg1	illness					910:916	no reported respiratory illness	886:916	no reported respiratory illness	886:916	(2) Methods: We used stool microbiota composition, metabolites including organic acids and gut health markers in several machine-learning-based classification tools related prospectively to experiencing reported bronchitis or LRTI, as compared to no reported respiratory illness.
34576834	6	11	theme	fucosylated	1249:1259	arg1	glycans					1261:1267	fucosylated glycans	1249:1267	fucosylated glycans	1249:1267	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	6	11	theme	fucosylated	1249:1259	arg1	FL					1220:1221	2'FL	1218:1221	2'FL	1218:1221	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	13	12	theme	observed	2021:2028	arg1	outcomes					2039:2046	the observed clinical outcomes	2017:2046	the observed clinical outcomes of reduced risk for bronchitis and LRTI in infants fed a formula with HMOs	2017:2121	(4) Conclusions: An early-life gut ecology characterized by Bifidobacterium-species-driven metabolic changes partly explains the observed clinical outcomes of reduced risk for bronchitis and LRTI in infants fed a formula with HMOs.
34576834	10	13	theme	B.	1731:1732	arg1	subsp					1741:1745	B. longum subsp	1731:1745	B. longum subsp	1731:1745	In vitro experiments demonstrate that 2'FL, LNnT and lacto-N-tetraose (LNT) stimulated B. longum subsp.
34576834	5	14	theme	preclinical	932:942	arg1	function					963:970	preclinical epithelial barrier function	932:970	preclinical epithelial barrier function	932:970	We performed preclinical epithelial barrier function modelling to add mechanistic insight to these clinical observations.
34576834	6	15	theme	=	1174:1174	arg1	n					1172:1172	n = 70/103	1172:1181	n = 70/103	1172:1181	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	6	15	theme	=	1174:1174	arg1	LRTI					1166:1169	LRTI	1166:1169	LRTI (n = 70/103)	1166:1182	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	0	16	theme	Later	84:88	arg1	Infections					108:117	Later Respiratory Tract Infections	84:117	Later Respiratory Tract Infections	84:117	Human Milk Oligosaccharide-Stimulated Bifidobacterium Species Contribute to Prevent Later Respiratory Tract Infections.
34576834	13	17	theme	Bifidobacterium-species-driven	1952:1981	arg1	changes					1993:1999	Bifidobacterium-species-driven metabolic changes	1952:1999	Bifidobacterium-species-driven metabolic changes	1952:1999	(4) Conclusions: An early-life gut ecology characterized by Bifidobacterium-species-driven metabolic changes partly explains the observed clinical outcomes of reduced risk for bronchitis and LRTI in infants fed a formula with HMOs.
34576834	4	18	theme	stool	660:664	arg1	composition					677:687	stool microbiota composition	660:687	stool microbiota composition	660:687	(2) Methods: We used stool microbiota composition, metabolites including organic acids and gut health markers in several machine-learning-based classification tools related prospectively to experiencing reported bronchitis or LRTI, as compared to no reported respiratory illness.
34576834	0	19	theme	Tract	102:106	arg1	Infections					108:117	Later Respiratory Tract Infections	84:117	Later Respiratory Tract Infections	84:117	Human Milk Oligosaccharide-Stimulated Bifidobacterium Species Contribute to Prevent Later Respiratory Tract Infections.
34576834	12	20	theme	culture	1810:1816	arg1	media					1818:1822	spent culture media	1804:1822	spent culture media	1804:1822	Metabolites in spent culture media, primarily due to acetate, supported epithelial barrier protection.
34576834	8	21	theme	univariate	1474:1483	arg1	analysis					1485:1492	univariate analysis	1474:1492	univariate analysis	1474:1492	By univariate analysis, infants experiencing no bronchitis or LRTI, compared with those who did, showed higher acetate (p < 0.007) and B. longum subsp.
34576834	4	22	theme	health	734:739	arg1	acids					720:724	organic acids	712:724	organic acids	712:724	(2) Methods: We used stool microbiota composition, metabolites including organic acids and gut health markers in several machine-learning-based classification tools related prospectively to experiencing reported bronchitis or LRTI, as compared to no reported respiratory illness.
34576834	4	22	theme	health	734:739	arg1	markers					741:747	gut health markers	730:747	gut health markers	730:747	(2) Methods: We used stool microbiota composition, metabolites including organic acids and gut health markers in several machine-learning-based classification tools related prospectively to experiencing reported bronchitis or LRTI, as compared to no reported respiratory illness.
34576834	6	23	theme	lower	1301:1305	arg1	succinate					1307:1315	lower succinate	1301:1315	lower succinate	1301:1315	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	6	23	theme	lower	1301:1305	arg1	FL					1220:1221	2'FL	1218:1221	2'FL	1218:1221	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	13	24	theme	gut	1923:1925	arg1	ecology					1927:1933	An early-life gut ecology	1909:1933	An early-life gut ecology characterized by Bifidobacterium-species-driven metabolic changes	1909:1999	(4) Conclusions: An early-life gut ecology characterized by Bifidobacterium-species-driven metabolic changes partly explains the observed clinical outcomes of reduced risk for bronchitis and LRTI in infants fed a formula with HMOs.
34576834	1	25	theme	milk	142:145	arg1	HMOs					165:168	HMOs	165:168	HMOs	165:168	(1) Background: Human milk oligosaccharides (HMOs) may support immune protection, partly via their action on the early-life gut microbiota.
34576834	1	25	theme	milk	142:145	arg1	oligosaccharides					147:162	Human milk oligosaccharides	136:162	Human milk oligosaccharides (HMOs)	136:169	(1) Background: Human milk oligosaccharides (HMOs) may support immune protection, partly via their action on the early-life gut microbiota.
34576834	1	26	dep	Background	124:133	arg1	1					121:121	1	121:121	1	121:121	(1) Background: Human milk oligosaccharides (HMOs) may support immune protection, partly via their action on the early-life gut microbiota.
34576834	1	26	dep	Background	124:133	arg1	support					175:181	support	175:181	support	175:181	(1) Background: Human milk oligosaccharides (HMOs) may support immune protection, partly via their action on the early-life gut microbiota.
34576834	4	27	used	used	655:658	arg2	We					652:653	We	652:653	We	652:653	(2) Methods: We used stool microbiota composition, metabolites including organic acids and gut health markers in several machine-learning-based classification tools related prospectively to experiencing reported bronchitis or LRTI, as compared to no reported respiratory illness.
34576834	13	28	dep	Conclusions	1896:1906	arg1	explains					2008:2015	explains	2008:2015	explains the observed clinical outcomes of reduced risk for bronchitis and LRTI in infants fed a formula with HMOs	2008:2121	(4) Conclusions: An early-life gut ecology characterized by Bifidobacterium-species-driven metabolic changes partly explains the observed clinical outcomes of reduced risk for bronchitis and LRTI in infants fed a formula with HMOs.
34576834	2	29	theme	bronchitis	435:444	arg1	LRTI					485:488	LRTI	485:488	LRTI	485:488	Exploratory findings of a randomized placebo-controlled trial associated 2'fucosyllactose (2'FL) and lacto-N-neotetraose (LNnT) formula feeding with reduced risk for reported bronchitis and lower respiratory tract illnesses (LRTI), as well as changes in gut microbiota composition.
34576834	2	29	theme	bronchitis	435:444	arg1	illnesses					474:482	reported bronchitis and lower respiratory tract illnesses	426:482	reported bronchitis and lower respiratory tract illnesses (LRTI)	426:489	Exploratory findings of a randomized placebo-controlled trial associated 2'fucosyllactose (2'FL) and lacto-N-neotetraose (LNnT) formula feeding with reduced risk for reported bronchitis and lower respiratory tract illnesses (LRTI), as well as changes in gut microbiota composition.
34576834	4	30	theme	organic	712:718	arg1	acids					720:724	organic acids	712:724	organic acids	712:724	(2) Methods: We used stool microbiota composition, metabolites including organic acids and gut health markers in several machine-learning-based classification tools related prospectively to experiencing reported bronchitis or LRTI, as compared to no reported respiratory illness.
34576834	0	31	theme	Human	0:4	arg1	Species					54:60	Human Milk Oligosaccharide-Stimulated Bifidobacterium Species	0:60	Human Milk Oligosaccharide-Stimulated Bifidobacterium Species	0:60	Human Milk Oligosaccharide-Stimulated Bifidobacterium Species Contribute to Prevent Later Respiratory Tract Infections.
34576834	5	32	theme	barrier	955:961	arg1	function					963:970	preclinical epithelial barrier function	932:970	preclinical epithelial barrier function	932:970	We performed preclinical epithelial barrier function modelling to add mechanistic insight to these clinical observations.
34576834	6	33	contain	containing	1207:1216	arg2	acetate					1240:1246	higher acetate	1233:1246	higher acetate	1233:1246	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	6	33	contain	containing	1207:1216	arg2	LNnT					1227:1230	LNnT	1227:1230	LNnT	1227:1230	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	6	33	contain	containing	1207:1216	arg2	glycans					1261:1267	fucosylated glycans	1249:1267	fucosylated glycans	1249:1267	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	6	33	contain	containing	1207:1216	arg1	discriminant					1078:1089	discriminant	1078:1089	discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103)	1078:1182	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	6	33	contain	containing	1207:1216	arg2	Bifidobacterium					1273:1287	Bifidobacterium	1273:1287	Bifidobacterium	1273:1287	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	6	33	contain	containing	1207:1216	arg2	succinate					1307:1315	lower succinate	1301:1315	lower succinate	1301:1315	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	6	33	contain	containing	1207:1216	arg1	Results					1045:1051	Results	1045:1051	Results	1045:1051	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	6	33	contain	containing	1207:1216	arg2	5-aminovalerate					1343:1357	5-aminovalerate	1343:1357	5-aminovalerate	1343:1357	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	6	33	contain	containing	1207:1216	arg2	butyrate					1318:1325	butyrate	1318:1325	butyrate	1318:1325	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	6	33	contain	containing	1207:1216	arg1	formula					1199:1205	the 2-HMO formula	1189:1205	the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia	1189:1411	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	6	33	contain	containing	1207:1216	arg2	propionate					1328:1337	propionate	1328:1337	propionate	1328:1337	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	6	33	contain	containing	1207:1216	arg2	FL					1220:1221	2'FL	1218:1221	2'FL	1218:1221	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	6	34	theme	main	1064:1067	arg1	features					1069:1076	the main features	1060:1076	the main features	1060:1076	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	2	35	theme	reduced	409:415	arg1	risk					417:420	reduced risk	409:420	reduced risk for reported bronchitis and lower respiratory tract illnesses (LRTI), as well as changes in gut microbiota composition	409:539	Exploratory findings of a randomized placebo-controlled trial associated 2'fucosyllactose (2'FL) and lacto-N-neotetraose (LNnT) formula feeding with reduced risk for reported bronchitis and lower respiratory tract illnesses (LRTI), as well as changes in gut microbiota composition.
34576834	0	36	theme	Oligosaccharide-Stimulated	11:36	arg1	Species					54:60	Human Milk Oligosaccharide-Stimulated Bifidobacterium Species	0:60	Human Milk Oligosaccharide-Stimulated Bifidobacterium Species	0:60	Human Milk Oligosaccharide-Stimulated Bifidobacterium Species Contribute to Prevent Later Respiratory Tract Infections.
34576834	11	37	dep	activity	1779:1786	arg1	infantis					1748:1755	infantis	1748:1755	infantis (ATCC15697)	1748:1767	infantis (ATCC15697) metabolic activity.
34576834	11	37	dep	activity	1779:1786	arg1	ATCC15697					1758:1766	ATCC15697	1758:1766	ATCC15697	1758:1766	infantis (ATCC15697) metabolic activity.
34576834	2	38	theme	tract	468:472	arg1	LRTI					485:488	LRTI	485:488	LRTI	485:488	Exploratory findings of a randomized placebo-controlled trial associated 2'fucosyllactose (2'FL) and lacto-N-neotetraose (LNnT) formula feeding with reduced risk for reported bronchitis and lower respiratory tract illnesses (LRTI), as well as changes in gut microbiota composition.
34576834	2	38	theme	tract	468:472	arg1	illnesses					474:482	reported bronchitis and lower respiratory tract illnesses	426:482	reported bronchitis and lower respiratory tract illnesses (LRTI)	426:489	Exploratory findings of a randomized placebo-controlled trial associated 2'fucosyllactose (2'FL) and lacto-N-neotetraose (LNnT) formula feeding with reduced risk for reported bronchitis and lower respiratory tract illnesses (LRTI), as well as changes in gut microbiota composition.
34576834	2	39	theme	lower	450:454	arg1	tract					468:472	lower respiratory tract	450:472	lower respiratory tract	450:472	Exploratory findings of a randomized placebo-controlled trial associated 2'fucosyllactose (2'FL) and lacto-N-neotetraose (LNnT) formula feeding with reduced risk for reported bronchitis and lower respiratory tract illnesses (LRTI), as well as changes in gut microbiota composition.
34576834	3	40	theme	clinical	616:623	arg1	observations					625:636	these clinical observations	610:636	these clinical observations	610:636	We sought to identify putative gut microbial mechanisms linked with these clinical observations.
34576834	6	41	theme	reported	1130:1137	arg1	n					1151:1151	n = 80/106	1151:1160	n = 80/106	1151:1160	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	6	41	theme	reported	1130:1137	arg1	bronchitis					1139:1148	any reported bronchitis	1126:1148	any reported bronchitis (n = 80/106)	1126:1161	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	4	42	theme	machine-learning-based	760:781	arg1	tools					798:802	several machine-learning-based classification tools	752:802	several machine-learning-based classification tools related prospectively to experiencing reported bronchitis or LRTI	752:868	(2) Methods: We used stool microbiota composition, metabolites including organic acids and gut health markers in several machine-learning-based classification tools related prospectively to experiencing reported bronchitis or LRTI, as compared to no reported respiratory illness.
34576834	13	43	theme	early-life	1912:1921	arg1	ecology					1927:1933	An early-life gut ecology	1909:1933	An early-life gut ecology characterized by Bifidobacterium-species-driven metabolic changes	1909:1999	(4) Conclusions: An early-life gut ecology characterized by Bifidobacterium-species-driven metabolic changes partly explains the observed clinical outcomes of reduced risk for bronchitis and LRTI in infants fed a formula with HMOs.
34576834	2	44	from	changes	503:509	arg1	composition					529:539	gut microbiota composition	514:539	gut microbiota composition	514:539	Exploratory findings of a randomized placebo-controlled trial associated 2'fucosyllactose (2'FL) and lacto-N-neotetraose (LNnT) formula feeding with reduced risk for reported bronchitis and lower respiratory tract illnesses (LRTI), as well as changes in gut microbiota composition.
34576834	6	45	theme	Carnobacteriaceae	1371:1387	arg1	members					1389:1395	Carnobacteriaceae members	1371:1395	Carnobacteriaceae members	1371:1395	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	11	46	theme	metabolic	1769:1777	arg1	activity					1779:1786	metabolic activity	1769:1786	metabolic activity	1769:1786	infantis (ATCC15697) metabolic activity.
34576834	7	47	theme	Bifidobacterium	1446:1460	arg1	species					1462:1468	several Bifidobacterium species	1438:1468	several Bifidobacterium species	1438:1468	Acetate correlated with several Bifidobacterium species.
34576834	14	48	theme	Trial	2125:2129	arg1	number					2140:2145	Trial registry number NCT01715246	2125:2157	Trial registry number NCT01715246.	2125:2158	(Trial registry number NCT01715246.)
34576834	6	49	theme	higher	1233:1238	arg1	acetate					1240:1246	higher acetate	1233:1246	higher acetate	1233:1246	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	6	49	theme	higher	1233:1238	arg1	FL					1220:1221	2'FL	1218:1221	2'FL	1218:1221	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	1	50	theme	gut	244:246	arg1	microbiota					248:257	the early-life gut microbiota	229:257	the early-life gut microbiota	229:257	(1) Background: Human milk oligosaccharides (HMOs) may support immune protection, partly via their action on the early-life gut microbiota.
34576834	2	51	theme	randomized	286:295	arg1	trial					316:320	a randomized placebo-controlled trial	284:320	a randomized placebo-controlled trial	284:320	Exploratory findings of a randomized placebo-controlled trial associated 2'fucosyllactose (2'FL) and lacto-N-neotetraose (LNnT) formula feeding with reduced risk for reported bronchitis and lower respiratory tract illnesses (LRTI), as well as changes in gut microbiota composition.
34576834	6	52	dep	formula	1199:1205	arg1	3					1042:1042	3	1042:1042	3	1042:1042	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	8	53	theme	p	1591:1591	arg1	0.007					1595:1599	p < 0.007	1591:1599	p < 0.007	1591:1599	By univariate analysis, infants experiencing no bronchitis or LRTI, compared with those who did, showed higher acetate (p < 0.007) and B. longum subsp.
34576834	8	53	theme	p	1591:1591	arg1	acetate					1582:1588	higher acetate	1575:1588	higher acetate (p < 0.007)	1575:1600	By univariate analysis, infants experiencing no bronchitis or LRTI, compared with those who did, showed higher acetate (p < 0.007) and B. longum subsp.
34576834	13	54	from	outcomes	2039:2046	arg1	infants					2091:2097	infants	2091:2097	infants fed a formula with HMOs	2091:2121	(4) Conclusions: An early-life gut ecology characterized by Bifidobacterium-species-driven metabolic changes partly explains the observed clinical outcomes of reduced risk for bronchitis and LRTI in infants fed a formula with HMOs.
34576834	6	55	gly	fucosylated	1249:1259	arg1	glycans					1261:1267	fucosylated glycans	1249:1267	fucosylated glycans	1249:1267	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	6	55	gly	fucosylated	1249:1259	arg1	FL					1220:1221	2'FL	1218:1221	2'FL	1218:1221	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	4	56	theme	reported	842:849	arg1	bronchitis					851:860	bronchitis	851:860	bronchitis	851:860	(2) Methods: We used stool microbiota composition, metabolites including organic acids and gut health markers in several machine-learning-based classification tools related prospectively to experiencing reported bronchitis or LRTI, as compared to no reported respiratory illness.
34576834	1	57	theme	immune	183:188	arg1	protection					190:199	immune protection	183:199	immune protection	183:199	(1) Background: Human milk oligosaccharides (HMOs) may support immune protection, partly via their action on the early-life gut microbiota.
34576834	6	58	theme	=	1153:1153	arg1	n					1151:1151	n = 80/106	1151:1160	n = 80/106	1151:1160	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	6	58	theme	=	1153:1153	arg1	bronchitis					1139:1148	any reported bronchitis	1126:1148	any reported bronchitis (n = 80/106)	1126:1161	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	10	59	dep	B.	1731:1732	arg1	longum					1734:1739	longum	1734:1739	longum	1734:1739	In vitro experiments demonstrate that 2'FL, LNnT and lacto-N-tetraose (LNT) stimulated B. longum subsp.
34576834	2	60	theme	trial	316:320	arg1	findings					272:279	Exploratory findings	260:279	Exploratory findings of a randomized placebo-controlled trial	260:320	Exploratory findings of a randomized placebo-controlled trial associated 2'fucosyllactose (2'FL) and lacto-N-neotetraose (LNnT) formula feeding with reduced risk for reported bronchitis and lower respiratory tract illnesses (LRTI), as well as changes in gut microbiota composition.
34576834	12	61	theme	barrier	1872:1878	arg1	protection					1880:1889	epithelial barrier protection	1861:1889	epithelial barrier protection	1861:1889	Metabolites in spent culture media, primarily due to acetate, supported epithelial barrier protection.
34576834	2	62	theme	gut	514:516	arg1	composition					529:539	gut microbiota composition	514:539	gut microbiota composition	514:539	Exploratory findings of a randomized placebo-controlled trial associated 2'fucosyllactose (2'FL) and lacto-N-neotetraose (LNnT) formula feeding with reduced risk for reported bronchitis and lower respiratory tract illnesses (LRTI), as well as changes in gut microbiota composition.
34576834	3	63	theme	putative	564:571	arg1	mechanisms					587:596	putative gut microbial mechanisms	564:596	putative gut microbial mechanisms linked with these clinical observations	564:636	We sought to identify putative gut microbial mechanisms linked with these clinical observations.
34576834	6	64	theme	2-HMO	1193:1197	arg1	Results					1045:1051	Results	1045:1051	Results	1045:1051	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	6	64	theme	2-HMO	1193:1197	arg1	discriminant					1078:1089	discriminant	1078:1089	discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103)	1078:1182	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	6	64	theme	2-HMO	1193:1197	arg1	formula					1199:1205	the 2-HMO formula	1189:1205	the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia	1189:1411	(3) Results: Among the main features discriminant for infants who did not experience any reported bronchitis (n = 80/106) or LRTI (n = 70/103) were the 2-HMO formula containing 2'FL and LNnT, higher acetate, fucosylated glycans and Bifidobacterium, as well as lower succinate, butyrate, propionate and 5-aminovalerate, along with Carnobacteriaceae members and Escherichia.
34576834	13	65	theme	risk	2059:2062	arg1	outcomes					2039:2046	the observed clinical outcomes	2017:2046	the observed clinical outcomes of reduced risk for bronchitis and LRTI in infants fed a formula with HMOs	2017:2121	(4) Conclusions: An early-life gut ecology characterized by Bifidobacterium-species-driven metabolic changes partly explains the observed clinical outcomes of reduced risk for bronchitis and LRTI in infants fed a formula with HMOs.
34576834	4	66	theme	microbiota	666:675	arg1	composition					677:687	stool microbiota composition	660:687	stool microbiota composition	660:687	(2) Methods: We used stool microbiota composition, metabolites including organic acids and gut health markers in several machine-learning-based classification tools related prospectively to experiencing reported bronchitis or LRTI, as compared to no reported respiratory illness.
34576834	1	67	from	action	219:224	arg1	microbiota					248:257	the early-life gut microbiota	229:257	the early-life gut microbiota	229:257	(1) Background: Human milk oligosaccharides (HMOs) may support immune protection, partly via their action on the early-life gut microbiota.
34576834	2	68	theme	Exploratory	260:270	arg1	findings					272:279	Exploratory findings	260:279	Exploratory findings of a randomized placebo-controlled trial	260:320	Exploratory findings of a randomized placebo-controlled trial associated 2'fucosyllactose (2'FL) and lacto-N-neotetraose (LNnT) formula feeding with reduced risk for reported bronchitis and lower respiratory tract illnesses (LRTI), as well as changes in gut microbiota composition.
34576834	1	69	theme	Human	136:140	arg1	HMOs					165:168	HMOs	165:168	HMOs	165:168	(1) Background: Human milk oligosaccharides (HMOs) may support immune protection, partly via their action on the early-life gut microbiota.
34576834	1	69	theme	Human	136:140	arg1	oligosaccharides					147:162	Human milk oligosaccharides	136:162	Human milk oligosaccharides (HMOs)	136:169	(1) Background: Human milk oligosaccharides (HMOs) may support immune protection, partly via their action on the early-life gut microbiota.
34576834	4	70	theme	respiratory	898:908	arg1	illness					910:916	no reported respiratory illness	886:916	no reported respiratory illness	886:916	(2) Methods: We used stool microbiota composition, metabolites including organic acids and gut health markers in several machine-learning-based classification tools related prospectively to experiencing reported bronchitis or LRTI, as compared to no reported respiratory illness.
34576834	8	71	dep	B.	1606:1607	arg1	longum					1609:1614	longum	1609:1614	longum	1609:1614	By univariate analysis, infants experiencing no bronchitis or LRTI, compared with those who did, showed higher acetate (p < 0.007) and B. longum subsp.
34576834	0	72	theme	Respiratory	90:100	arg1	Infections					108:117	Later Respiratory Tract Infections	84:117	Later Respiratory Tract Infections	84:117	Human Milk Oligosaccharide-Stimulated Bifidobacterium Species Contribute to Prevent Later Respiratory Tract Infections.
34576834	13	73	theme	clinical	2030:2037	arg1	outcomes					2039:2046	the observed clinical outcomes	2017:2046	the observed clinical outcomes of reduced risk for bronchitis and LRTI in infants fed a formula with HMOs	2017:2121	(4) Conclusions: An early-life gut ecology characterized by Bifidobacterium-species-driven metabolic changes partly explains the observed clinical outcomes of reduced risk for bronchitis and LRTI in infants fed a formula with HMOs.
34576834	3	74	theme	microbial	577:585	arg1	mechanisms					587:596	putative gut microbial mechanisms	564:596	putative gut microbial mechanisms linked with these clinical observations	564:636	We sought to identify putative gut microbial mechanisms linked with these clinical observations.
34576834	13	75	theme	metabolic	1983:1991	arg1	changes					1993:1999	Bifidobacterium-species-driven metabolic changes	1952:1999	Bifidobacterium-species-driven metabolic changes	1952:1999	(4) Conclusions: An early-life gut ecology characterized by Bifidobacterium-species-driven metabolic changes partly explains the observed clinical outcomes of reduced risk for bronchitis and LRTI in infants fed a formula with HMOs.
34576834	9	76	theme	p	1633:1633	arg1	≤					1635:1635	p ≤ 0.03	1633:1640	p ≤ 0.03	1633:1640	infantis (p ≤ 0.03).
34576834	9	76	theme	p	1633:1633	arg1	infantis					1623:1630	infantis	1623:1630	infantis (p ≤ 0.03).	1623:1642	infantis (p ≤ 0.03).
34576834	12	77	from	Metabolites	1789:1799	arg1	media					1818:1822	spent culture media	1804:1822	spent culture media	1804:1822	Metabolites in spent culture media, primarily due to acetate, supported epithelial barrier protection.
34576834	4	78	theme	gut	730:732	arg1	acids					720:724	organic acids	712:724	organic acids	712:724	(2) Methods: We used stool microbiota composition, metabolites including organic acids and gut health markers in several machine-learning-based classification tools related prospectively to experiencing reported bronchitis or LRTI, as compared to no reported respiratory illness.
34576834	4	78	theme	gut	730:732	arg1	markers					741:747	gut health markers	730:747	gut health markers	730:747	(2) Methods: We used stool microbiota composition, metabolites including organic acids and gut health markers in several machine-learning-based classification tools related prospectively to experiencing reported bronchitis or LRTI, as compared to no reported respiratory illness.
34576834	5	79	theme	mechanistic	989:999	arg1	insight					1001:1007	mechanistic insight	989:1007	mechanistic insight to these clinical observations	989:1038	We performed preclinical epithelial barrier function modelling to add mechanistic insight to these clinical observations.
34576834	10	80	dep	In	1644:1645	arg1	vitro					1647:1651	vitro	1647:1651	vitro	1647:1651	In vitro experiments demonstrate that 2'FL, LNnT and lacto-N-tetraose (LNT) stimulated B. longum subsp.
34576834	0	81	theme	Milk	6:9	arg1	Species					54:60	Human Milk Oligosaccharide-Stimulated Bifidobacterium Species	0:60	Human Milk Oligosaccharide-Stimulated Bifidobacterium Species	0:60	Human Milk Oligosaccharide-Stimulated Bifidobacterium Species Contribute to Prevent Later Respiratory Tract Infections.
34576834	12	82	theme	spent	1804:1808	arg1	media					1818:1822	spent culture media	1804:1822	spent culture media	1804:1822	Metabolites in spent culture media, primarily due to acetate, supported epithelial barrier protection.
34576834	5	83	theme	epithelial	944:953	arg1	function					963:970	preclinical epithelial barrier function	932:970	preclinical epithelial barrier function	932:970	We performed preclinical epithelial barrier function modelling to add mechanistic insight to these clinical observations.
34576834	2	84	theme	reported	426:433	arg1	LRTI					485:488	LRTI	485:488	LRTI	485:488	Exploratory findings of a randomized placebo-controlled trial associated 2'fucosyllactose (2'FL) and lacto-N-neotetraose (LNnT) formula feeding with reduced risk for reported bronchitis and lower respiratory tract illnesses (LRTI), as well as changes in gut microbiota composition.
34576834	2	84	theme	reported	426:433	arg1	illnesses					474:482	reported bronchitis and lower respiratory tract illnesses	426:482	reported bronchitis and lower respiratory tract illnesses (LRTI)	426:489	Exploratory findings of a randomized placebo-controlled trial associated 2'fucosyllactose (2'FL) and lacto-N-neotetraose (LNnT) formula feeding with reduced risk for reported bronchitis and lower respiratory tract illnesses (LRTI), as well as changes in gut microbiota composition.
34576834	0	85	theme	Bifidobacterium	38:52	arg1	Species					54:60	Human Milk Oligosaccharide-Stimulated Bifidobacterium Species	0:60	Human Milk Oligosaccharide-Stimulated Bifidobacterium Species	0:60	Human Milk Oligosaccharide-Stimulated Bifidobacterium Species Contribute to Prevent Later Respiratory Tract Infections.
34576834	2	86	theme	respiratory	456:466	arg1	tract					468:472	lower respiratory tract	450:472	lower respiratory tract	450:472	Exploratory findings of a randomized placebo-controlled trial associated 2'fucosyllactose (2'FL) and lacto-N-neotetraose (LNnT) formula feeding with reduced risk for reported bronchitis and lower respiratory tract illnesses (LRTI), as well as changes in gut microbiota composition.
34576834	4	87	theme	related	804:810	arg1	tools					798:802	several machine-learning-based classification tools	752:802	several machine-learning-based classification tools related prospectively to experiencing reported bronchitis or LRTI	752:868	(2) Methods: We used stool microbiota composition, metabolites including organic acids and gut health markers in several machine-learning-based classification tools related prospectively to experiencing reported bronchitis or LRTI, as compared to no reported respiratory illness.
34576834	2	88	theme	formula	388:394	arg1	feeding					396:402	formula feeding	388:402	formula feeding	388:402	Exploratory findings of a randomized placebo-controlled trial associated 2'fucosyllactose (2'FL) and lacto-N-neotetraose (LNnT) formula feeding with reduced risk for reported bronchitis and lower respiratory tract illnesses (LRTI), as well as changes in gut microbiota composition.
34576834	4	89	dep	Methods	643:649	arg1	used					655:658	used	655:658	used	655:658	(2) Methods: We used stool microbiota composition, metabolites including organic acids and gut health markers in several machine-learning-based classification tools related prospectively to experiencing reported bronchitis or LRTI, as compared to no reported respiratory illness.
34576834	4	89	dep	Methods	643:649	arg1	2					640:640	2	640:640	2	640:640	(2) Methods: We used stool microbiota composition, metabolites including organic acids and gut health markers in several machine-learning-based classification tools related prospectively to experiencing reported bronchitis or LRTI, as compared to no reported respiratory illness.
34576834	4	90	theme	several	752:758	arg1	tools					798:802	several machine-learning-based classification tools	752:802	several machine-learning-based classification tools related prospectively to experiencing reported bronchitis or LRTI	752:868	(2) Methods: We used stool microbiota composition, metabolites including organic acids and gut health markers in several machine-learning-based classification tools related prospectively to experiencing reported bronchitis or LRTI, as compared to no reported respiratory illness.
34576834	8	91	theme	B.	1606:1607	arg1	subsp					1616:1620	B. longum subsp	1606:1620	B. longum subsp	1606:1620	By univariate analysis, infants experiencing no bronchitis or LRTI, compared with those who did, showed higher acetate (p < 0.007) and B. longum subsp.
34576834	5	92	theme	clinical	1018:1025	arg1	observations					1027:1038	these clinical observations	1012:1038	these clinical observations	1012:1038	We performed preclinical epithelial barrier function modelling to add mechanistic insight to these clinical observations.
34576834	8	93	theme	higher	1575:1580	arg1	0.007					1595:1599	p < 0.007	1591:1599	p < 0.007	1591:1599	By univariate analysis, infants experiencing no bronchitis or LRTI, compared with those who did, showed higher acetate (p < 0.007) and B. longum subsp.
34576834	8	93	theme	higher	1575:1580	arg1	acetate					1582:1588	higher acetate	1575:1588	higher acetate (p < 0.007)	1575:1600	By univariate analysis, infants experiencing no bronchitis or LRTI, compared with those who did, showed higher acetate (p < 0.007) and B. longum subsp.
34576834	14	94	theme	registry	2131:2138	arg1	number					2140:2145	Trial registry number NCT01715246	2125:2157	Trial registry number NCT01715246.	2125:2158	(Trial registry number NCT01715246.)
34576834	4	95	theme	classification	783:796	arg1	tools					798:802	several machine-learning-based classification tools	752:802	several machine-learning-based classification tools related prospectively to experiencing reported bronchitis or LRTI	752:868	(2) Methods: We used stool microbiota composition, metabolites including organic acids and gut health markers in several machine-learning-based classification tools related prospectively to experiencing reported bronchitis or LRTI, as compared to no reported respiratory illness.
34576834	10	96	theme	In	1644:1645	arg1	experiments					1653:1663	In vitro experiments	1644:1663	In vitro experiments	1644:1663	In vitro experiments demonstrate that 2'FL, LNnT and lacto-N-tetraose (LNT) stimulated B. longum subsp.
34576834	1	97	theme	early-life	233:242	arg1	microbiota					248:257	the early-life gut microbiota	229:257	the early-life gut microbiota	229:257	(1) Background: Human milk oligosaccharides (HMOs) may support immune protection, partly via their action on the early-life gut microbiota.
34279376	0	0	theme	Selected	84:91	arg1	Species					103:109	Selected Microbial Species	84:109	Selected Microbial Species	84:109	Application of Green Algal Planktochlorella nurekis Biomasses to Modulate Growth of Selected Microbial Species.
34279376	4	1	theme	photo-reprogramming	708:726	arg1	phenomenon					694:703	the phenomenon	690:703	the phenomenon of photo-reprogramming of metabolism	690:740	as a modulator of the growth of microorganisms that can be used in the cosmetics and pharmaceutical industries by exploiting the phenomenon of photo-reprogramming of metabolism.
34279376	6	2	theme	PCM	1072:1074	arg1	bacteria					1010:1017	pathogenic bacteria	999:1017	pathogenic bacteria	999:1017	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	6	2	theme	PCM	1072:1074	arg1	458					1076:1078	Staphylococcus aureus PCM 458	1050:1078	Staphylococcus aureus PCM 458	1050:1078	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	1	3	theme	lipids	153:158	arg1	producers					130:138	producers	130:138	producers of proteins, lipids, polysaccharides, pigments, vitamins and unique secondary metabolites	130:228	As microalgae are producers of proteins, lipids, polysaccharides, pigments, vitamins and unique secondary metabolites, microalgal biotechnology has gained attention in recent decades.
34279376	1	3	theme	lipids	153:158	arg1	microalgae					115:124	microalgae	115:124	microalgae	115:124	As microalgae are producers of proteins, lipids, polysaccharides, pigments, vitamins and unique secondary metabolites, microalgal biotechnology has gained attention in recent decades.
34279376	4	4	theme	growth	587:592	arg1	modulator					570:578	a modulator	568:578	a modulator of the growth of microorganisms that can be used in the cosmetics and pharmaceutical industries by exploiting the phenomenon of photo-reprogramming of metabolism	568:740	as a modulator of the growth of microorganisms that can be used in the cosmetics and pharmaceutical industries by exploiting the phenomenon of photo-reprogramming of metabolism.
34279376	6	5	theme	pathogenic	999:1008	arg1	bacteria					1010:1017	pathogenic bacteria	999:1017	pathogenic bacteria	999:1017	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	6	5	theme	pathogenic	999:1008	arg1	PCM					1155:1157	Escherichia coli PCM 2209	1138:1162	Escherichia coli PCM 2209	1138:1162	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	6	5	theme	pathogenic	999:1008	arg1	2318					1108:1111	Streptococcus pyogenes PCM 2318	1081:1111	Streptococcus pyogenes PCM 2318	1081:1111	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	6	5	theme	pathogenic	999:1008	arg1	458					1076:1078	Staphylococcus aureus PCM 458	1050:1078	Staphylococcus aureus PCM 458	1050:1078	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	6	5	theme	pathogenic	999:1008	arg1	aeruginosa					1126:1135	Pseudomonas aeruginosa	1114:1135	Pseudomonas aeruginosa	1114:1135	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	6	5	theme	pathogenic	999:1008	arg1	faecalis					1040:1047	Enterococcus faecalis	1027:1047	Enterococcus faecalis	1027:1047	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	6	5	theme	pathogenic	999:1008	arg1	albicans					1176:1183	Candida albicans	1168:1183	Candida albicans	1168:1183	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	6	6	theme	water	931:935	arg1	extracts					937:944	The ethanolic and water extracts	913:944	extracts	937:944	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	8	7	theme	antibiotics	1364:1374	arg1	use					1357:1359	the use	1353:1359	the use of antibiotics and environmentally harmful chemicals commonly used in preparations against Enterococcus faecalis, Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa, Escherichia coli or Candida spp	1353:1576	The presented technology may allow the limitation of the use of antibiotics and environmentally harmful chemicals commonly used in preparations against Enterococcus faecalis, Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa, Escherichia coli or Candida spp.
34279376	6	8	theme	bacteria	1010:1017	arg1	number					989:994	a number	987:994	a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053	987:1194	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	0	9	theme	Species	103:109	arg1	Growth					74:79	Growth	74:79	Growth of Selected Microbial Species	74:109	Application of Green Algal Planktochlorella nurekis Biomasses to Modulate Growth of Selected Microbial Species.
34279376	8	10	theme	use	1357:1359	arg1	limitation					1339:1348	the limitation	1335:1348	the limitation of the use of antibiotics and environmentally harmful chemicals commonly used in preparations against Enterococcus faecalis, Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa, Escherichia coli or Candida spp	1335:1576	The presented technology may allow the limitation of the use of antibiotics and environmentally harmful chemicals commonly used in preparations against Enterococcus faecalis, Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa, Escherichia coli or Candida spp.
34279376	3	11	theme	sp	561:562	arg1	microalgae					520:529	unicellular microalgae	508:529	unicellular microalgae of the genus Planktochlorella sp	508:562	Here, we present the application of a method of producing a natural, biologically active composite obtained from unicellular microalgae of the genus Planktochlorella sp.
34279376	4	12	theme	microorganisms	597:610	arg1	growth					587:592	the growth	583:592	the growth of microorganisms that can be used in the cosmetics and pharmaceutical industries by exploiting the phenomenon of photo-reprogramming of metabolism	583:740	as a modulator of the growth of microorganisms that can be used in the cosmetics and pharmaceutical industries by exploiting the phenomenon of photo-reprogramming of metabolism.
34279376	0	13	theme	Microbial	93:101	arg1	Species					103:109	Selected Microbial Species	84:109	Selected Microbial Species	84:109	Application of Green Algal Planktochlorella nurekis Biomasses to Modulate Growth of Selected Microbial Species.
34279376	6	14	theme	ethanolic	917:925	arg1	extracts					937:944	The ethanolic and water extracts	913:944	extracts	937:944	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	3	15	theme	unicellular	508:518	arg1	microalgae					520:529	unicellular microalgae	508:529	unicellular microalgae of the genus Planktochlorella sp	508:562	Here, we present the application of a method of producing a natural, biologically active composite obtained from unicellular microalgae of the genus Planktochlorella sp.
34279376	7	16	theme	prebiotic	1278:1286	arg1	supplement					1288:1297	a prebiotic supplement	1276:1297	a prebiotic supplement	1276:1297	The algal biocomposite obtained according to our procedure can be used also as a prebiotic supplement.
34279376	7	16	theme	prebiotic	1278:1286	arg1	biocomposite					1207:1218	The algal biocomposite	1197:1218	The algal biocomposite obtained according to our procedure	1197:1254	The algal biocomposite obtained according to our procedure can be used also as a prebiotic supplement.
34279376	5	17	theme	red	762:764	arg1	light					775:779	red and blue light	762:779	red and blue light	762:779	The combination of red and blue light allows the collection of biomass with unique biochemical profiles, especially fatty acid composition (Patent Application P.429620).
34279376	8	18	theme	Candida	1566:1572	arg1	spp					1574:1576	Candida spp	1566:1576	Candida spp	1566:1576	The presented technology may allow the limitation of the use of antibiotics and environmentally harmful chemicals commonly used in preparations against Enterococcus faecalis, Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa, Escherichia coli or Candida spp.
34279376	6	19	theme	PCM	1104:1106	arg1	bacteria					1010:1017	pathogenic bacteria	999:1017	pathogenic bacteria	999:1017	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	6	19	theme	PCM	1104:1106	arg1	2318					1108:1111	Streptococcus pyogenes PCM 2318	1081:1111	Streptococcus pyogenes PCM 2318	1081:1111	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	3	20	theme	genus	538:542	arg1	sp					561:562	the genus Planktochlorella sp	534:562	the genus Planktochlorella sp	534:562	Here, we present the application of a method of producing a natural, biologically active composite obtained from unicellular microalgae of the genus Planktochlorella sp.
34279376	5	21	theme	Application	890:900	arg1	composition					870:880	fatty acid composition	859:880	fatty acid composition (Patent Application P.429620)	859:910	The combination of red and blue light allows the collection of biomass with unique biochemical profiles, especially fatty acid composition (Patent Application P.429620).
34279376	5	21	theme	Application	890:900	arg1	P.429620					902:909	Patent Application P.429620	883:909	Patent Application P.429620	883:909	The combination of red and blue light allows the collection of biomass with unique biochemical profiles, especially fatty acid composition (Patent Application P.429620).
34279376	1	22	theme	polysaccharides	161:175	arg1	producers					130:138	producers	130:138	producers of proteins, lipids, polysaccharides, pigments, vitamins and unique secondary metabolites	130:228	As microalgae are producers of proteins, lipids, polysaccharides, pigments, vitamins and unique secondary metabolites, microalgal biotechnology has gained attention in recent decades.
34279376	1	22	theme	polysaccharides	161:175	arg1	microalgae					115:124	microalgae	115:124	microalgae	115:124	As microalgae are producers of proteins, lipids, polysaccharides, pigments, vitamins and unique secondary metabolites, microalgal biotechnology has gained attention in recent decades.
34279376	8	23	theme	presented	1304:1312	arg1	technology					1314:1323	The presented technology	1300:1323	The presented technology	1300:1323	The presented technology may allow the limitation of the use of antibiotics and environmentally harmful chemicals commonly used in preparations against Enterococcus faecalis, Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa, Escherichia coli or Candida spp.
34279376	3	24	theme	Planktochlorella	544:559	arg1	sp					561:562	the genus Planktochlorella sp	534:562	the genus Planktochlorella sp	534:562	Here, we present the application of a method of producing a natural, biologically active composite obtained from unicellular microalgae of the genus Planktochlorella sp.
34279376	4	25	theme	pharmaceutical	650:663	arg1	industries					665:674	pharmaceutical industries	650:674	pharmaceutical industries	650:674	as a modulator of the growth of microorganisms that can be used in the cosmetics and pharmaceutical industries by exploiting the phenomenon of photo-reprogramming of metabolism.
34279376	5	26	theme	unique	819:824	arg1	composition					870:880	fatty acid composition	859:880	fatty acid composition (Patent Application P.429620)	859:910	The combination of red and blue light allows the collection of biomass with unique biochemical profiles, especially fatty acid composition (Patent Application P.429620).
34279376	5	26	theme	unique	819:824	arg1	profiles					838:845	unique biochemical profiles	819:845	unique biochemical profiles	819:845	The combination of red and blue light allows the collection of biomass with unique biochemical profiles, especially fatty acid composition (Patent Application P.429620).
34279376	6	27	dep	faecalis	1040:1047	arg1	ATCC					1185:1188	ATCC 14053	1185:1194	ATCC 14053	1185:1194	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	6	28	theme	number	989:994	arg1	growth					977:982	the growth	973:982	the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053	973:1194	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	6	29	dep	Streptococcus	1081:1093	arg1	pyogenes					1095:1102	pyogenes	1095:1102	pyogenes	1095:1102	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	6	30	theme	Streptococcus	1081:1093	arg1	bacteria					1010:1017	pathogenic bacteria	999:1017	pathogenic bacteria	999:1017	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	6	30	theme	Streptococcus	1081:1093	arg1	2318					1108:1111	Streptococcus pyogenes PCM 2318	1081:1111	Streptococcus pyogenes PCM 2318	1081:1111	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	1	31	theme	pigments	178:185	arg1	producers					130:138	producers	130:138	producers of proteins, lipids, polysaccharides, pigments, vitamins and unique secondary metabolites	130:228	As microalgae are producers of proteins, lipids, polysaccharides, pigments, vitamins and unique secondary metabolites, microalgal biotechnology has gained attention in recent decades.
34279376	1	31	theme	pigments	178:185	arg1	microalgae					115:124	microalgae	115:124	microalgae	115:124	As microalgae are producers of proteins, lipids, polysaccharides, pigments, vitamins and unique secondary metabolites, microalgal biotechnology has gained attention in recent decades.
34279376	5	32	theme	biochemical	826:836	arg1	composition					870:880	fatty acid composition	859:880	fatty acid composition (Patent Application P.429620)	859:910	The combination of red and blue light allows the collection of biomass with unique biochemical profiles, especially fatty acid composition (Patent Application P.429620).
34279376	5	32	theme	biochemical	826:836	arg1	profiles					838:845	unique biochemical profiles	819:845	unique biochemical profiles	819:845	The combination of red and blue light allows the collection of biomass with unique biochemical profiles, especially fatty acid composition (Patent Application P.429620).
34279376	1	33	theme	recent	280:285	arg1	decades					287:293	recent decades	280:293	recent decades	280:293	As microalgae are producers of proteins, lipids, polysaccharides, pigments, vitamins and unique secondary metabolites, microalgal biotechnology has gained attention in recent decades.
34279376	6	34	theme	Candida	1168:1174	arg1	bacteria					1010:1017	pathogenic bacteria	999:1017	pathogenic bacteria	999:1017	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	6	34	theme	Candida	1168:1174	arg1	albicans					1176:1183	Candida albicans	1168:1183	Candida albicans	1168:1183	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	3	35	theme	method	433:438	arg1	application					416:426	the application	412:426	the application of a method of producing a natural, biologically active composite obtained from unicellular microalgae of the genus Planktochlorella sp	412:562	Here, we present the application of a method of producing a natural, biologically active composite obtained from unicellular microalgae of the genus Planktochlorella sp.
34279376	0	36	theme	Algal	21:25	arg1	nurekis					44:50	Green Algal Planktochlorella nurekis	15:50	Green Algal Planktochlorella nurekis	15:50	Application of Green Algal Planktochlorella nurekis Biomasses to Modulate Growth of Selected Microbial Species.
34279376	6	37	theme	aureus	1065:1070	arg1	bacteria					1010:1017	pathogenic bacteria	999:1017	pathogenic bacteria	999:1017	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	6	37	theme	aureus	1065:1070	arg1	458					1076:1078	Staphylococcus aureus PCM 458	1050:1078	Staphylococcus aureus PCM 458	1050:1078	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	1	38	theme	vitamins	188:195	arg1	producers					130:138	producers	130:138	producers of proteins, lipids, polysaccharides, pigments, vitamins and unique secondary metabolites	130:228	As microalgae are producers of proteins, lipids, polysaccharides, pigments, vitamins and unique secondary metabolites, microalgal biotechnology has gained attention in recent decades.
34279376	1	38	theme	vitamins	188:195	arg1	microalgae					115:124	microalgae	115:124	microalgae	115:124	As microalgae are producers of proteins, lipids, polysaccharides, pigments, vitamins and unique secondary metabolites, microalgal biotechnology has gained attention in recent decades.
34279376	0	39	theme	Green	15:19	arg1	nurekis					44:50	Green Algal Planktochlorella nurekis	15:50	Green Algal Planktochlorella nurekis	15:50	Application of Green Algal Planktochlorella nurekis Biomasses to Modulate Growth of Selected Microbial Species.
34279376	4	40	used	used	624:627	arg2	microorganisms					597:610	microorganisms	597:610	microorganisms that can be used in the cosmetics and pharmaceutical industries by exploiting the phenomenon of photo-reprogramming of metabolism	597:740	as a modulator of the growth of microorganisms that can be used in the cosmetics and pharmaceutical industries by exploiting the phenomenon of photo-reprogramming of metabolism.
34279376	8	41	theme	harmful	1396:1402	arg1	chemicals					1404:1412	environmentally harmful chemicals	1380:1412	environmentally harmful chemicals	1380:1412	The presented technology may allow the limitation of the use of antibiotics and environmentally harmful chemicals commonly used in preparations against Enterococcus faecalis, Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa, Escherichia coli or Candida spp.
34279376	5	42	theme	fatty	859:863	arg1	composition					870:880	fatty acid composition	859:880	fatty acid composition (Patent Application P.429620)	859:910	The combination of red and blue light allows the collection of biomass with unique biochemical profiles, especially fatty acid composition (Patent Application P.429620).
34279376	5	42	theme	fatty	859:863	arg1	profiles					838:845	unique biochemical profiles	819:845	unique biochemical profiles	819:845	The combination of red and blue light allows the collection of biomass with unique biochemical profiles, especially fatty acid composition (Patent Application P.429620).
34279376	5	42	theme	fatty	859:863	arg1	P.429620					902:909	Patent Application P.429620	883:909	Patent Application P.429620	883:909	The combination of red and blue light allows the collection of biomass with unique biochemical profiles, especially fatty acid composition (Patent Application P.429620).
34279376	0	43	theme	nurekis	44:50	arg1	Application					0:10	Application	0:10	Application of Green Algal Planktochlorella nurekis	0:50	Application of Green Algal Planktochlorella nurekis Biomasses to Modulate Growth of Selected Microbial Species.
34279376	5	44	theme	acid	865:868	arg1	composition					870:880	fatty acid composition	859:880	fatty acid composition (Patent Application P.429620)	859:910	The combination of red and blue light allows the collection of biomass with unique biochemical profiles, especially fatty acid composition (Patent Application P.429620).
34279376	5	44	theme	acid	865:868	arg1	profiles					838:845	unique biochemical profiles	819:845	unique biochemical profiles	819:845	The combination of red and blue light allows the collection of biomass with unique biochemical profiles, especially fatty acid composition (Patent Application P.429620).
34279376	5	44	theme	acid	865:868	arg1	P.429620					902:909	Patent Application P.429620	883:909	Patent Application P.429620	883:909	The combination of red and blue light allows the collection of biomass with unique biochemical profiles, especially fatty acid composition (Patent Application P.429620).
34279376	1	45	theme	unique	201:206	arg1	metabolites					218:228	unique secondary metabolites	201:228	unique secondary metabolites	201:228	As microalgae are producers of proteins, lipids, polysaccharides, pigments, vitamins and unique secondary metabolites, microalgal biotechnology has gained attention in recent decades.
34279376	0	46	theme	Planktochlorella	27:42	arg1	nurekis					44:50	Green Algal Planktochlorella nurekis	15:50	Green Algal Planktochlorella nurekis	15:50	Application of Green Algal Planktochlorella nurekis Biomasses to Modulate Growth of Selected Microbial Species.
34279376	1	47	theme	secondary	208:216	arg1	metabolites					218:228	unique secondary metabolites	201:228	unique secondary metabolites	201:228	As microalgae are producers of proteins, lipids, polysaccharides, pigments, vitamins and unique secondary metabolites, microalgal biotechnology has gained attention in recent decades.
34279376	5	48	theme	biomass	806:812	arg1	collection					792:801	the collection	788:801	the collection of biomass	788:812	The combination of red and blue light allows the collection of biomass with unique biochemical profiles, especially fatty acid composition (Patent Application P.429620).
34279376	6	49	theme	Staphylococcus	1050:1063	arg1	bacteria					1010:1017	pathogenic bacteria	999:1017	pathogenic bacteria	999:1017	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	6	49	theme	Staphylococcus	1050:1063	arg1	458					1076:1078	Staphylococcus aureus PCM 458	1050:1078	Staphylococcus aureus PCM 458	1050:1078	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	4	50	theme	metabolism	731:740	arg1	photo-reprogramming					708:726	photo-reprogramming	708:726	photo-reprogramming of metabolism	708:740	as a modulator of the growth of microorganisms that can be used in the cosmetics and pharmaceutical industries by exploiting the phenomenon of photo-reprogramming of metabolism.
34279376	6	51	theme	biomass	955:961	arg1	extracts					937:944	The ethanolic and water extracts	913:944	extracts	937:944	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	6	52	theme	coli	1150:1153	arg1	bacteria					1010:1017	pathogenic bacteria	999:1017	pathogenic bacteria	999:1017	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	6	52	theme	coli	1150:1153	arg1	PCM					1155:1157	Escherichia coli PCM 2209	1138:1162	Escherichia coli PCM 2209	1138:1162	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	6	53	theme	algae	949:953	arg1	biomass					955:961	algae biomass	949:961	algae biomass	949:961	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	7	54	used	used	1263:1266	arg2	supplement					1288:1297	a prebiotic supplement	1276:1297	a prebiotic supplement	1276:1297	The algal biocomposite obtained according to our procedure can be used also as a prebiotic supplement.
34279376	7	54	used	used	1263:1266	arg2	biocomposite					1207:1218	The algal biocomposite	1197:1218	The algal biocomposite obtained according to our procedure	1197:1254	The algal biocomposite obtained according to our procedure can be used also as a prebiotic supplement.
34279376	6	55	theme	Escherichia	1138:1148	arg1	bacteria					1010:1017	pathogenic bacteria	999:1017	pathogenic bacteria	999:1017	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	6	55	theme	Escherichia	1138:1148	arg1	PCM					1155:1157	Escherichia coli PCM 2209	1138:1162	Escherichia coli PCM 2209	1138:1162	The ethanolic and water extracts of algae biomass inhibited the growth of a number of pathogenic bacteria, namely Enterococcus faecalis, Staphylococcus aureus PCM 458, Streptococcus pyogenes PCM 2318, Pseudomonas aeruginosa, Escherichia coli PCM 2209 and Candida albicans ATCC 14053.
34279376	8	56	theme	chemicals	1404:1412	arg1	use					1357:1359	the use	1353:1359	the use of antibiotics and environmentally harmful chemicals commonly used in preparations against Enterococcus faecalis, Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa, Escherichia coli or Candida spp	1353:1576	The presented technology may allow the limitation of the use of antibiotics and environmentally harmful chemicals commonly used in preparations against Enterococcus faecalis, Staphylococcus aureus, Streptococcus pyogenes, Pseudomonas aeruginosa, Escherichia coli or Candida spp.
34279376	1	57	theme	metabolites	218:228	arg1	producers					130:138	producers	130:138	producers of proteins, lipids, polysaccharides, pigments, vitamins and unique secondary metabolites	130:228	As microalgae are producers of proteins, lipids, polysaccharides, pigments, vitamins and unique secondary metabolites, microalgal biotechnology has gained attention in recent decades.
34279376	1	57	theme	metabolites	218:228	arg1	microalgae					115:124	microalgae	115:124	microalgae	115:124	As microalgae are producers of proteins, lipids, polysaccharides, pigments, vitamins and unique secondary metabolites, microalgal biotechnology has gained attention in recent decades.
34279376	3	58	dep	natural	455:461	arg1	active					477:482	active	477:482	active	477:482	Here, we present the application of a method of producing a natural, biologically active composite obtained from unicellular microalgae of the genus Planktochlorella sp.
34279376	2	59	theme	biomass	323:329	arg1	production					331:340	biomass production	323:340	biomass production	323:340	Microalgae can be used for biomass production and to obtain biotechnologically important products.
34279376	2	60	used	used	314:317	arg2	Microalgae					296:305	Microalgae	296:305	Microalgae	296:305	Microalgae can be used for biomass production and to obtain biotechnologically important products.
34279376	5	61	theme	blue	770:773	arg1	light					775:779	red and blue light	762:779	red and blue light	762:779	The combination of red and blue light allows the collection of biomass with unique biochemical profiles, especially fatty acid composition (Patent Application P.429620).
34279376	3	62	theme	natural	455:461	arg1	composite					484:492	a natural, biologically active composite	453:492	a natural, biologically active composite obtained from unicellular microalgae of the genus Planktochlorella sp	453:562	Here, we present the application of a method of producing a natural, biologically active composite obtained from unicellular microalgae of the genus Planktochlorella sp.
34279376	1	63	theme	proteins	143:150	arg1	producers					130:138	producers	130:138	producers of proteins, lipids, polysaccharides, pigments, vitamins and unique secondary metabolites	130:228	As microalgae are producers of proteins, lipids, polysaccharides, pigments, vitamins and unique secondary metabolites, microalgal biotechnology has gained attention in recent decades.
34279376	1	63	theme	proteins	143:150	arg1	microalgae					115:124	microalgae	115:124	microalgae	115:124	As microalgae are producers of proteins, lipids, polysaccharides, pigments, vitamins and unique secondary metabolites, microalgal biotechnology has gained attention in recent decades.
34279376	5	64	theme	Patent	883:888	arg1	composition					870:880	fatty acid composition	859:880	fatty acid composition (Patent Application P.429620)	859:910	The combination of red and blue light allows the collection of biomass with unique biochemical profiles, especially fatty acid composition (Patent Application P.429620).
34279376	5	64	theme	Patent	883:888	arg1	P.429620					902:909	Patent Application P.429620	883:909	Patent Application P.429620	883:909	The combination of red and blue light allows the collection of biomass with unique biochemical profiles, especially fatty acid composition (Patent Application P.429620).
34279376	1	65	theme	microalgal	231:240	arg1	biotechnology					242:254	microalgal biotechnology	231:254	microalgal biotechnology	231:254	As microalgae are producers of proteins, lipids, polysaccharides, pigments, vitamins and unique secondary metabolites, microalgal biotechnology has gained attention in recent decades.
34279376	5	66	theme	light	775:779	arg1	combination					747:757	The combination	743:757	The combination of red and blue light	743:779	The combination of red and blue light allows the collection of biomass with unique biochemical profiles, especially fatty acid composition (Patent Application P.429620).
34279376	7	67	theme	algal	1201:1205	arg1	supplement					1288:1297	a prebiotic supplement	1276:1297	a prebiotic supplement	1276:1297	The algal biocomposite obtained according to our procedure can be used also as a prebiotic supplement.
34279376	7	67	theme	algal	1201:1205	arg1	biocomposite					1207:1218	The algal biocomposite	1197:1218	The algal biocomposite obtained according to our procedure	1197:1254	The algal biocomposite obtained according to our procedure can be used also as a prebiotic supplement.
34279376	2	68	theme	important	375:383	arg1	products					385:392	biotechnologically important products	356:392	biotechnologically important products	356:392	Microalgae can be used for biomass production and to obtain biotechnologically important products.
32600680	2	0	theme	different	121:129	arg1	sponge					184:189	a solid sponge	176:189	a solid sponge (SS)	176:194	In the present study, two different PLGA-Alginate scaffolds, a hydrogel (HY) and a solid sponge (SS), were developed for β-estradiol and BMP-2 sustained delivery for bone regeneration in osteoporosis.
32600680	2	0	theme	different	121:129	arg1	scaffolds					145:153	two different PLGA-Alginate scaffolds	117:153	two different PLGA-Alginate scaffolds	117:153	In the present study, two different PLGA-Alginate scaffolds, a hydrogel (HY) and a solid sponge (SS), were developed for β-estradiol and BMP-2 sustained delivery for bone regeneration in osteoporosis.
32600680	2	0	theme	different	121:129	arg1	hydrogel					158:165	a hydrogel	156:165	a hydrogel (HY)	156:170	In the present study, two different PLGA-Alginate scaffolds, a hydrogel (HY) and a solid sponge (SS), were developed for β-estradiol and BMP-2 sustained delivery for bone regeneration in osteoporosis.
32600680	6	1	theme	rat	679:681	arg1	model					683:687	an osteoporotic (OP) rat model	658:687	an osteoporotic (OP) rat model	658:687	The bone regeneration induced by both HY and SS was evaluated using a critical-sized bone defect in an osteoporotic (OP) rat model.
32600680	4	2	theme	properties	495:504	arg1	terms					430:434	terms	430:434	terms of porosity, water uptake, release rate and HY rheological properties	430:504	Scaffolds were characterized in vitro in terms of porosity, water uptake, release rate and HY rheological properties.
32600680	7	3	theme	water	757:761	arg1	absorption					763:772	double water absorption	750:772	double water absorption	750:772	Compared to HY, SS presented 30% higher porosity, more than double water absorption capacity and almost negligible mass loss compared to the 40% of HY.
32600680	1	4	from	regeneration	65:76	arg1	osteoporosis					81:92	osteoporosis	81:92	osteoporosis	81:92	Efficacy in bone regeneration in osteoporosis.
32600680	4	5	theme	rheological	483:493	arg1	properties					495:504	HY rheological properties	480:504	HY rheological properties	480:504	Scaffolds were characterized in vitro in terms of porosity, water uptake, release rate and HY rheological properties.
32600680	3	6	theme	PLGA	339:342	arg1	microspheres					362:373	PLGA and PLGA-Alginate microspheres	339:373	microspheres	362:373	β-Estradiol and BMP-2 were encapsulated in PLGA and PLGA-Alginate microspheres respectively.
32600680	10	7	theme	OP	1202:1203	arg1	rats					1205:1208	OP rats	1202:1208	OP rats	1202:1208	The incorporation of mesenchymal stem cells obtained from OP rats did not result in any improvement or synergistic effect on bone repair.
32600680	9	8	theme	release	1009:1015	arg1	profile					1017:1023	release profile	1009:1023	release profile	1009:1023	Despite both delivery systems had similar composition and release profile, bone repair was around 30% higher with SS than with HY, possibly due to its longer residence time at the defect site.
32600680	7	9	theme	negligible	794:803	arg1	loss					810:813	almost negligible mass loss	787:813	almost negligible mass loss	787:813	Compared to HY, SS presented 30% higher porosity, more than double water absorption capacity and almost negligible mass loss compared to the 40% of HY.
32600680	9	10	theme	delivery	964:971	arg1	systems					973:979	both delivery systems	959:979	both delivery systems had similar composition and release profile	959:1023	Despite both delivery systems had similar composition and release profile, bone repair was around 30% higher with SS than with HY, possibly due to its longer residence time at the defect site.
32600680	4	11	theme	uptake	455:460	arg1	terms					430:434	terms	430:434	terms of porosity, water uptake, release rate and HY rheological properties	430:504	Scaffolds were characterized in vitro in terms of porosity, water uptake, release rate and HY rheological properties.
32600680	2	12	theme	sustained	238:246	arg1	delivery					248:255	sustained delivery	238:255	sustained delivery	238:255	In the present study, two different PLGA-Alginate scaffolds, a hydrogel (HY) and a solid sponge (SS), were developed for β-estradiol and BMP-2 sustained delivery for bone regeneration in osteoporosis.
32600680	6	13	theme	OP	675:676	arg1	model					683:687	an osteoporotic (OP) rat model	658:687	an osteoporotic (OP) rat model	658:687	The bone regeneration induced by both HY and SS was evaluated using a critical-sized bone defect in an osteoporotic (OP) rat model.
32600680	4	14	theme	rate	471:474	arg1	terms					430:434	terms	430:434	terms of porosity, water uptake, release rate and HY rheological properties	430:504	Scaffolds were characterized in vitro in terms of porosity, water uptake, release rate and HY rheological properties.
32600680	10	15	theme	synergistic	1247:1257	arg1	effect					1259:1264	synergistic effect	1247:1264	synergistic effect on bone repair	1247:1279	The incorporation of mesenchymal stem cells obtained from OP rats did not result in any improvement or synergistic effect on bone repair.
32600680	4	16	theme	release	463:469	arg1	rate					471:474	release rate	463:474	release rate	463:474	Scaffolds were characterized in vitro in terms of porosity, water uptake, release rate and HY rheological properties.
32600680	10	17	theme	bone	1269:1272	arg1	repair					1274:1279	bone repair	1269:1279	bone repair	1269:1279	The incorporation of mesenchymal stem cells obtained from OP rats did not result in any improvement or synergistic effect on bone repair.
32600680	6	18	from	defect	648:653	arg1	model					683:687	an osteoporotic (OP) rat model	658:687	an osteoporotic (OP) rat model	658:687	The bone regeneration induced by both HY and SS was evaluated using a critical-sized bone defect in an osteoporotic (OP) rat model.
32600680	6	19	theme	osteoporotic	661:672	arg1	model					683:687	an osteoporotic (OP) rat model	658:687	an osteoporotic (OP) rat model	658:687	The bone regeneration induced by both HY and SS was evaluated using a critical-sized bone defect in an osteoporotic (OP) rat model.
32600680	7	20	theme	more	740:743	arg1	capacity					774:781	more than double water absorption capacity	740:781	more than double water absorption capacity	740:781	Compared to HY, SS presented 30% higher porosity, more than double water absorption capacity and almost negligible mass loss compared to the 40% of HY.
32600680	10	21	theme	cells	1182:1186	arg1	incorporation					1148:1160	The incorporation	1144:1160	The incorporation of mesenchymal stem cells obtained from OP rats	1144:1208	The incorporation of mesenchymal stem cells obtained from OP rats did not result in any improvement or synergistic effect on bone repair.
32600680	2	22	dep	β-estradiol	216:226	arg1	delivery					248:255	sustained delivery	238:255	sustained delivery	238:255	In the present study, two different PLGA-Alginate scaffolds, a hydrogel (HY) and a solid sponge (SS), were developed for β-estradiol and BMP-2 sustained delivery for bone regeneration in osteoporosis.
32600680	10	23	theme	mesenchymal	1165:1175	arg1	cells					1182:1186	mesenchymal stem cells	1165:1186	mesenchymal stem cells obtained from OP rats	1165:1208	The incorporation of mesenchymal stem cells obtained from OP rats did not result in any improvement or synergistic effect on bone repair.
32600680	8	24	theme	defect	886:891	arg1	shape					893:897	the defect shape	882:897	the defect shape	882:897	Both systems were flexible and fit well the defect shape, however, HY has the advantage of being injectable.
32600680	3	25	theme	PLGA-Alginate	348:360	arg1	microspheres					362:373	PLGA and PLGA-Alginate microspheres	339:373	microspheres	362:373	β-Estradiol and BMP-2 were encapsulated in PLGA and PLGA-Alginate microspheres respectively.
32600680	9	26	theme	similar	985:991	arg1	composition					993:1003	similar composition	985:1003	similar composition	985:1003	Despite both delivery systems had similar composition and release profile, bone repair was around 30% higher with SS than with HY, possibly due to its longer residence time at the defect site.
32600680	4	27	theme	porosity	439:446	arg1	terms					430:434	terms	430:434	terms of porosity, water uptake, release rate and HY rheological properties	430:504	Scaffolds were characterized in vitro in terms of porosity, water uptake, release rate and HY rheological properties.
32600680	2	28	theme	bone	261:264	arg1	regeneration					266:277	bone regeneration	261:277	bone regeneration in osteoporosis	261:293	In the present study, two different PLGA-Alginate scaffolds, a hydrogel (HY) and a solid sponge (SS), were developed for β-estradiol and BMP-2 sustained delivery for bone regeneration in osteoporosis.
32600680	7	29	theme	double	750:755	arg1	absorption					763:772	double water absorption	750:772	double water absorption	750:772	Compared to HY, SS presented 30% higher porosity, more than double water absorption capacity and almost negligible mass loss compared to the 40% of HY.
32600680	6	30	theme	bone	562:565	arg1	regeneration					567:578	The bone regeneration	558:578	The bone regeneration induced by both HY and SS	558:604	The bone regeneration induced by both HY and SS was evaluated using a critical-sized bone defect in an osteoporotic (OP) rat model.
32600680	7	31	theme	mass	805:808	arg1	loss					810:813	almost negligible mass loss	787:813	almost negligible mass loss	787:813	Compared to HY, SS presented 30% higher porosity, more than double water absorption capacity and almost negligible mass loss compared to the 40% of HY.
32600680	6	32	theme	bone	643:646	arg1	defect					648:653	a critical-sized bone defect	626:653	a critical-sized bone defect in an osteoporotic (OP) rat model	626:687	The bone regeneration induced by both HY and SS was evaluated using a critical-sized bone defect in an osteoporotic (OP) rat model.
32600680	0	33	theme	alginate-solid	25:38	arg1	system					40:45	alginate-solid system	25:45	alginate-solid system	25:45	Alginate-hydrogel versus alginate-solid system.
32600680	4	34	theme	water	449:453	arg1	uptake					455:460	water uptake	449:460	water uptake	449:460	Scaffolds were characterized in vitro in terms of porosity, water uptake, release rate and HY rheological properties.
32600680	1	35	dep	Efficacy	48:55	arg1	regeneration					65:76	bone regeneration	60:76	bone regeneration in osteoporosis	60:92	Efficacy in bone regeneration in osteoporosis.
32600680	9	36	from	site	1138:1141	arg1	time					1119:1122	its longer residence time	1098:1122	its longer residence time at the defect site	1098:1141	Despite both delivery systems had similar composition and release profile, bone repair was around 30% higher with SS than with HY, possibly due to its longer residence time at the defect site.
32600680	2	37	theme	present	102:108	arg1	study					110:114	the present study	98:114	the present study	98:114	In the present study, two different PLGA-Alginate scaffolds, a hydrogel (HY) and a solid sponge (SS), were developed for β-estradiol and BMP-2 sustained delivery for bone regeneration in osteoporosis.
32600680	9	38	theme	residence	1109:1117	arg1	time					1119:1122	its longer residence time	1098:1122	its longer residence time at the defect site	1098:1141	Despite both delivery systems had similar composition and release profile, bone repair was around 30% higher with SS than with HY, possibly due to its longer residence time at the defect site.
32600680	9	39	theme	bone	1026:1029	arg1	repair					1031:1036	bone repair	1026:1036	bone repair	1026:1036	Despite both delivery systems had similar composition and release profile, bone repair was around 30% higher with SS than with HY, possibly due to its longer residence time at the defect site.
32600680	10	40	from	improvement	1232:1242	arg1	repair					1274:1279	bone repair	1269:1279	bone repair	1269:1279	The incorporation of mesenchymal stem cells obtained from OP rats did not result in any improvement or synergistic effect on bone repair.
32600680	4	41	theme	HY	480:481	arg1	properties					495:504	HY rheological properties	480:504	HY rheological properties	480:504	Scaffolds were characterized in vitro in terms of porosity, water uptake, release rate and HY rheological properties.
32600680	9	42	theme	longer	1102:1107	arg1	time					1119:1122	its longer residence time	1098:1122	its longer residence time at the defect site	1098:1141	Despite both delivery systems had similar composition and release profile, bone repair was around 30% higher with SS than with HY, possibly due to its longer residence time at the defect site.
32600680	6	43	theme	critical-sized	628:641	arg1	defect					648:653	a critical-sized bone defect	626:653	a critical-sized bone defect in an osteoporotic (OP) rat model	626:687	The bone regeneration induced by both HY and SS was evaluated using a critical-sized bone defect in an osteoporotic (OP) rat model.
32600680	7	44	theme	HY	838:839	arg1	%					833:833	the 40%	827:833	the 40% of HY	827:839	Compared to HY, SS presented 30% higher porosity, more than double water absorption capacity and almost negligible mass loss compared to the 40% of HY.
32600680	7	44	theme	HY	838:839	arg1	HY					838:839	HY	838:839	HY	838:839	Compared to HY, SS presented 30% higher porosity, more than double water absorption capacity and almost negligible mass loss compared to the 40% of HY.
32600680	2	45	theme	solid	178:182	arg1	SS					192:193	SS	192:193	SS	192:193	In the present study, two different PLGA-Alginate scaffolds, a hydrogel (HY) and a solid sponge (SS), were developed for β-estradiol and BMP-2 sustained delivery for bone regeneration in osteoporosis.
32600680	2	45	theme	solid	178:182	arg1	sponge					184:189	a solid sponge	176:189	a solid sponge (SS)	176:194	In the present study, two different PLGA-Alginate scaffolds, a hydrogel (HY) and a solid sponge (SS), were developed for β-estradiol and BMP-2 sustained delivery for bone regeneration in osteoporosis.
32600680	2	45	theme	solid	178:182	arg1	scaffolds					145:153	two different PLGA-Alginate scaffolds	117:153	two different PLGA-Alginate scaffolds	117:153	In the present study, two different PLGA-Alginate scaffolds, a hydrogel (HY) and a solid sponge (SS), were developed for β-estradiol and BMP-2 sustained delivery for bone regeneration in osteoporosis.
32600680	10	46	theme	stem	1177:1180	arg1	cells					1182:1186	mesenchymal stem cells	1165:1186	mesenchymal stem cells obtained from OP rats	1165:1208	The incorporation of mesenchymal stem cells obtained from OP rats did not result in any improvement or synergistic effect on bone repair.
32600680	8	47	contain	has	912:914	arg1	shape					893:897	the defect shape	882:897	the defect shape	882:897	Both systems were flexible and fit well the defect shape, however, HY has the advantage of being injectable.
32600680	8	47	contain	has	912:914	arg1	HY					909:910	HY	909:910	HY	909:910	Both systems were flexible and fit well the defect shape, however, HY has the advantage of being injectable.
32600680	8	47	contain	has	912:914	arg2	advantage					920:928	the advantage	916:928	the advantage of being injectable	916:948	Both systems were flexible and fit well the defect shape, however, HY has the advantage of being injectable.
32600680	9	48	theme	defect	1131:1136	arg1	site					1138:1141	the defect site	1127:1141	the defect site	1127:1141	Despite both delivery systems had similar composition and release profile, bone repair was around 30% higher with SS than with HY, possibly due to its longer residence time at the defect site.
32600680	5	49	theme	BMP-2	507:511	arg1	profiles					521:528	BMP-2 release profiles	507:528	BMP-2 release profiles	507:528	BMP-2 release profiles were also analysed in vivo.
32600680	2	50	theme	PLGA-Alginate	131:143	arg1	sponge					184:189	a solid sponge	176:189	a solid sponge (SS)	176:194	In the present study, two different PLGA-Alginate scaffolds, a hydrogel (HY) and a solid sponge (SS), were developed for β-estradiol and BMP-2 sustained delivery for bone regeneration in osteoporosis.
32600680	2	50	theme	PLGA-Alginate	131:143	arg1	scaffolds					145:153	two different PLGA-Alginate scaffolds	117:153	two different PLGA-Alginate scaffolds	117:153	In the present study, two different PLGA-Alginate scaffolds, a hydrogel (HY) and a solid sponge (SS), were developed for β-estradiol and BMP-2 sustained delivery for bone regeneration in osteoporosis.
32600680	2	50	theme	PLGA-Alginate	131:143	arg1	hydrogel					158:165	a hydrogel	156:165	a hydrogel (HY)	156:170	In the present study, two different PLGA-Alginate scaffolds, a hydrogel (HY) and a solid sponge (SS), were developed for β-estradiol and BMP-2 sustained delivery for bone regeneration in osteoporosis.
32600680	1	51	theme	bone	60:63	arg1	regeneration					65:76	bone regeneration	60:76	bone regeneration in osteoporosis	60:92	Efficacy in bone regeneration in osteoporosis.
32600680	5	52	theme	release	513:519	arg1	profiles					521:528	BMP-2 release profiles	507:528	BMP-2 release profiles	507:528	BMP-2 release profiles were also analysed in vivo.
32600680	10	53	from	effect	1259:1264	arg1	repair					1274:1279	bone repair	1269:1279	bone repair	1269:1279	The incorporation of mesenchymal stem cells obtained from OP rats did not result in any improvement or synergistic effect on bone repair.
32600680	2	54	from	regeneration	266:277	arg1	osteoporosis					282:293	osteoporosis	282:293	osteoporosis	282:293	In the present study, two different PLGA-Alginate scaffolds, a hydrogel (HY) and a solid sponge (SS), were developed for β-estradiol and BMP-2 sustained delivery for bone regeneration in osteoporosis.
32600680	7	55	theme	higher	723:728	arg1	porosity					730:737	30% higher porosity	719:737	30% higher porosity	719:737	Compared to HY, SS presented 30% higher porosity, more than double water absorption capacity and almost negligible mass loss compared to the 40% of HY.
32559834	6	0	theme	%	803:803	arg1	values					784:789	digital DNA-DNA hybridization values	754:789	digital DNA-DNA hybridization values of 32.3-22.3%	754:803	Genome analysis of the strain with type strains of the other Cutibacterium species resulted in digital DNA-DNA hybridization values of 32.3-22.3% and average nucleotide identity (OrthoANI) values of 86.7-73.6 %.
32559834	6	0	theme	%	803:803	arg1	values					848:853	average nucleotide identity (OrthoANI) values	809:853	average nucleotide identity (OrthoANI) values of 86.7-73.6 %	809:868	Genome analysis of the strain with type strains of the other Cutibacterium species resulted in digital DNA-DNA hybridization values of 32.3-22.3% and average nucleotide identity (OrthoANI) values of 86.7-73.6 %.
32559834	4	1	theme	agar	392:395	arg1	plates					397:402	trypticase soy agar plates	377:402	trypticase soy agar plates supplemented with 5 % sheep blood	377:436	Growth on trypticase soy agar plates supplemented with 5 % sheep blood was fastest at 30-37 °C under anaerobic conditions.
32559834	2	2	theme	glands	275:280	arg1	meibum					240:245	the meibum	236:245	the meibum of inflamed human meibomian glands	236:280	An anaerobic and aerotolerant bacterium, strain M12T, was isolated from the meibum of inflamed human meibomian glands.
32559834	16	3	theme	further	1628:1634	arg1	analysis					1644:1651	further genomic analysis	1628:1651	further genomic analysis	1628:1651	On the basis of further genomic analysis, we also provide emended descriptions of Cutibacterium granulosum (Prévot 1938) Scholz and Kilian 2016 and Cutibacterium namnetense (Aubin et al. 2016) Nouioui et al. 2018.
32559834	12	4	theme	MALDI-TOF	1359:1367	arg1	spectrum					1387:1394	the MALDI-TOF mass spectrometry spectrum	1355:1394	the MALDI-TOF mass spectrometry spectrum	1355:1394	The major peaks of the MALDI-TOF mass spectrometry spectrum were at 3493, 3712, 6986 and 7424 Da.
32559834	6	5	theme	hybridization	770:782	arg1	values					784:789	digital DNA-DNA hybridization values	754:789	digital DNA-DNA hybridization values of 32.3-22.3%	754:803	Genome analysis of the strain with type strains of the other Cutibacterium species resulted in digital DNA-DNA hybridization values of 32.3-22.3% and average nucleotide identity (OrthoANI) values of 86.7-73.6 %.
32559834	2	6	theme	human	259:263	arg1	glands					275:280	inflamed human meibomian glands	250:280	inflamed human meibomian glands	250:280	An anaerobic and aerotolerant bacterium, strain M12T, was isolated from the meibum of inflamed human meibomian glands.
32559834	5	7	theme	rRNA	498:501	arg1	sequence					508:515	The 16S rRNA gene sequence	490:515	The 16S rRNA gene sequence of the strain	490:529	The 16S rRNA gene sequence of the strain revealed that it belongs to the genus Cutibacterium with a 98.0 % similarity value to the closest species, Cutibacterium acnes.
32559834	16	8	theme	Kilian	1744:1749	arg1	descriptions					1678:1689	emended descriptions	1670:1689	emended descriptions of Cutibacterium granulosum (Prévot 1938) Scholz and Kilian 2016 and Cutibacterium namnetense (Aubin et al. 2016) Nouioui et al. 2018	1670:1823	On the basis of further genomic analysis, we also provide emended descriptions of Cutibacterium granulosum (Prévot 1938) Scholz and Kilian 2016 and Cutibacterium namnetense (Aubin et al. 2016) Nouioui et al. 2018.
32559834	12	9	theme	spectrometry	1374:1385	arg1	spectrum					1387:1394	the MALDI-TOF mass spectrometry spectrum	1355:1394	the MALDI-TOF mass spectrometry spectrum	1355:1394	The major peaks of the MALDI-TOF mass spectrometry spectrum were at 3493, 3712, 6986 and 7424 Da.
32559834	6	10	theme	digital	754:760	arg1	hybridization					770:782	digital DNA-DNA hybridization	754:782	digital DNA-DNA hybridization values of 32.3-22.3%	754:803	Genome analysis of the strain with type strains of the other Cutibacterium species resulted in digital DNA-DNA hybridization values of 32.3-22.3% and average nucleotide identity (OrthoANI) values of 86.7-73.6 %.
32559834	5	11	with	Cutibacterium	569:581	arg1	value					608:612	a 98.0 % similarity value	588:612	a 98.0 % similarity value	588:612	The 16S rRNA gene sequence of the strain revealed that it belongs to the genus Cutibacterium with a 98.0 % similarity value to the closest species, Cutibacterium acnes.
32559834	4	12	theme	trypticase	377:386	arg1	plates					397:402	trypticase soy agar plates	377:402	trypticase soy agar plates supplemented with 5 % sheep blood	377:436	Growth on trypticase soy agar plates supplemented with 5 % sheep blood was fastest at 30-37 °C under anaerobic conditions.
32559834	15	13	theme	33380T=DSM	1591:1600	arg1	M12T					1580:1583	M12T	1580:1583	M12T (=JCM 33380T=DSM 109769T)	1580:1609	The type strain of C. modestum is M12T (=JCM 33380T=DSM 109769T).
32559834	15	13	theme	33380T=DSM	1591:1600	arg1	109769T					1602:1608	=JCM 33380T=DSM 109769T	1586:1608	=JCM 33380T=DSM 109769T	1586:1608	The type strain of C. modestum is M12T (=JCM 33380T=DSM 109769T).
32559834	14	14	theme	novel	1507:1511	arg1	modestum					1536:1543	Cutibacterium modestum	1522:1543	Cutibacterium modestum	1522:1543	Based on these findings, we propose a novel species, Cutibacterium modestum.
32559834	14	14	theme	novel	1507:1511	arg1	species					1513:1519	a novel species	1505:1519	a novel species	1505:1519	Based on these findings, we propose a novel species, Cutibacterium modestum.
32559834	16	15	theme	Cutibacterium	1694:1706	arg1	descriptions					1678:1689	emended descriptions	1670:1689	emended descriptions of Cutibacterium granulosum (Prévot 1938) Scholz and Kilian 2016 and Cutibacterium namnetense (Aubin et al. 2016) Nouioui et al. 2018	1670:1823	On the basis of further genomic analysis, we also provide emended descriptions of Cutibacterium granulosum (Prévot 1938) Scholz and Kilian 2016 and Cutibacterium namnetense (Aubin et al. 2016) Nouioui et al. 2018.
32559834	16	16	dep	analysis	1644:1651	arg1	the					1615:1617	the	1615:1617	the	1615:1617	On the basis of further genomic analysis, we also provide emended descriptions of Cutibacterium granulosum (Prévot 1938) Scholz and Kilian 2016 and Cutibacterium namnetense (Aubin et al. 2016) Nouioui et al. 2018.
32559834	16	16	dep	analysis	1644:1651	arg1	basis					1619:1623	basis	1619:1623	basis	1619:1623	On the basis of further genomic analysis, we also provide emended descriptions of Cutibacterium granulosum (Prévot 1938) Scholz and Kilian 2016 and Cutibacterium namnetense (Aubin et al. 2016) Nouioui et al. 2018.
32559834	4	17	theme	5 	422:423	arg1	%					424:424	%	424:424	%	424:424	Growth on trypticase soy agar plates supplemented with 5 % sheep blood was fastest at 30-37 °C under anaerobic conditions.
32559834	10	18	theme	diagnostic	1208:1217	arg1	acid					1277:1280	meso-diaminopimelic acid	1257:1280	meso-diaminopimelic acid	1257:1280	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
32559834	10	18	theme	diagnostic	1208:1217	arg1	acid					1227:1230	The diagnostic diamino acid	1204:1230	The diagnostic diamino acid of the peptidoglycan	1204:1251	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
32559834	1	19	theme	granulosum	123:132	arg1	descriptions					93:104	emended descriptions	85:104	emended descriptions of Cutibacterium granulosum and Cutibacterium	85:150	nov., isolated from meibum of human meibomian glands, and emended descriptions of Cutibacterium granulosum and Cutibacterium namnetense.
32559834	1	19	theme	granulosum	123:132	arg1	nov.					27:30	nov.	27:30	nov.	27:30	nov., isolated from meibum of human meibomian glands, and emended descriptions of Cutibacterium granulosum and Cutibacterium namnetense.
32559834	4	20	theme	sheep	426:430	arg1	blood					432:436	5 % sheep blood	422:436	5 % sheep blood	422:436	Growth on trypticase soy agar plates supplemented with 5 % sheep blood was fastest at 30-37 °C under anaerobic conditions.
32559834	13	21	theme	59.9 mol	1458:1465	arg1	content					1446:1452	The DNA G+C content	1434:1452	The DNA G+C content	1434:1452	The DNA G+C content was 59.9 mol%.
32559834	13	21	theme	59.9 mol	1458:1465	arg1	%					1466:1466	59.9 mol%	1458:1466	59.9 mol%	1458:1466	The DNA G+C content was 59.9 mol%.
32559834	1	22	theme	Cutibacterium	138:150	arg1	descriptions					93:104	emended descriptions	85:104	emended descriptions of Cutibacterium granulosum and Cutibacterium	85:150	nov., isolated from meibum of human meibomian glands, and emended descriptions of Cutibacterium granulosum and Cutibacterium namnetense.
32559834	1	22	theme	Cutibacterium	138:150	arg1	nov.					27:30	nov.	27:30	nov.	27:30	nov., isolated from meibum of human meibomian glands, and emended descriptions of Cutibacterium granulosum and Cutibacterium namnetense.
32559834	7	23	theme	API	937:939	arg1	Coryne					941:946	API Coryne	937:946	API Coryne	937:946	Biochemical and physiological analyses using API rapid ID 32A and API Coryne kits revealed relatively low reactivity of the strain compared with C. acnes and Cutibacterium namnetense.
32559834	5	24	theme	98.0 	590:594	arg1	%					595:595	%	595:595	%	595:595	The 16S rRNA gene sequence of the strain revealed that it belongs to the genus Cutibacterium with a 98.0 % similarity value to the closest species, Cutibacterium acnes.
32559834	6	25	theme	strain	682:687	arg1	analysis					666:673	Genome analysis	659:673	Genome analysis of the strain with type strains of the other Cutibacterium species	659:740	Genome analysis of the strain with type strains of the other Cutibacterium species resulted in digital DNA-DNA hybridization values of 32.3-22.3% and average nucleotide identity (OrthoANI) values of 86.7-73.6 %.
32559834	7	26	theme	rapid	920:924	arg1	32A					929:931	API rapid ID 32A	916:931	API rapid ID 32A	916:931	Biochemical and physiological analyses using API rapid ID 32A and API Coryne kits revealed relatively low reactivity of the strain compared with C. acnes and Cutibacterium namnetense.
32559834	1	27	theme	meibomian	63:71	arg1	glands					73:78	human meibomian glands	57:78	human meibomian glands	57:78	nov., isolated from meibum of human meibomian glands, and emended descriptions of Cutibacterium granulosum and Cutibacterium namnetense.
32559834	16	28	dep	Aubin	1786:1790	arg1	al.					1795:1797	Aubin et al.	1786:1797	Aubin et al. 2016	1786:1802	On the basis of further genomic analysis, we also provide emended descriptions of Cutibacterium granulosum (Prévot 1938) Scholz and Kilian 2016 and Cutibacterium namnetense (Aubin et al. 2016) Nouioui et al. 2018.
32559834	16	28	dep	Aubin	1786:1790	arg1	2016					1799:1802	2016	1799:1802	2016	1799:1802	On the basis of further genomic analysis, we also provide emended descriptions of Cutibacterium granulosum (Prévot 1938) Scholz and Kilian 2016 and Cutibacterium namnetense (Aubin et al. 2016) Nouioui et al. 2018.
32559834	7	29	theme	32A	929:931	arg1	kits					948:951	API rapid ID 32A and API Coryne kits	916:951	API rapid ID 32A and API Coryne kits	916:951	Biochemical and physiological analyses using API rapid ID 32A and API Coryne kits revealed relatively low reactivity of the strain compared with C. acnes and Cutibacterium namnetense.
32559834	11	30	theme	Major	1283:1287	arg1	menaquinones					1289:1300	Major menaquinones	1283:1300	Major menaquinones	1283:1300	Major menaquinones were MK-9(H4), MK-9(H2) and MK-9.
32559834	6	31	theme	%	868:868	arg1	values					784:789	digital DNA-DNA hybridization values	754:789	digital DNA-DNA hybridization values of 32.3-22.3%	754:803	Genome analysis of the strain with type strains of the other Cutibacterium species resulted in digital DNA-DNA hybridization values of 32.3-22.3% and average nucleotide identity (OrthoANI) values of 86.7-73.6 %.
32559834	6	31	theme	%	868:868	arg1	values					848:853	average nucleotide identity (OrthoANI) values	809:853	average nucleotide identity (OrthoANI) values of 86.7-73.6 %	809:868	Genome analysis of the strain with type strains of the other Cutibacterium species resulted in digital DNA-DNA hybridization values of 32.3-22.3% and average nucleotide identity (OrthoANI) values of 86.7-73.6 %.
32559834	0	32	theme	Cutibacterium	0:12	arg1	sp					23:24	Cutibacterium modestum sp	0:24	Cutibacterium modestum sp.	0:25	Cutibacterium modestum sp.
32559834	5	33	theme	genus	563:567	arg1	Cutibacterium					569:581	the genus Cutibacterium	559:581	the genus Cutibacterium with a 98.0 % similarity value to the closest species, Cutibacterium acnes	559:656	The 16S rRNA gene sequence of the strain revealed that it belongs to the genus Cutibacterium with a 98.0 % similarity value to the closest species, Cutibacterium acnes.
32559834	13	34	theme	G+C	1442:1444	arg1	content					1446:1452	The DNA G+C content	1434:1452	The DNA G+C content	1434:1452	The DNA G+C content was 59.9 mol%.
32559834	13	34	theme	G+C	1442:1444	arg1	%					1466:1466	59.9 mol%	1458:1466	59.9 mol%	1458:1466	The DNA G+C content was 59.9 mol%.
32559834	6	35	theme	Genome	659:664	arg1	analysis					666:673	Genome analysis	659:673	Genome analysis of the strain with type strains of the other Cutibacterium species	659:740	Genome analysis of the strain with type strains of the other Cutibacterium species resulted in digital DNA-DNA hybridization values of 32.3-22.3% and average nucleotide identity (OrthoANI) values of 86.7-73.6 %.
32559834	1	36	theme	emended	85:91	arg1	descriptions					93:104	emended descriptions	85:104	emended descriptions of Cutibacterium granulosum and Cutibacterium	85:150	nov., isolated from meibum of human meibomian glands, and emended descriptions of Cutibacterium granulosum and Cutibacterium namnetense.
32559834	16	37	theme	emended	1670:1676	arg1	descriptions					1678:1689	emended descriptions	1670:1689	emended descriptions of Cutibacterium granulosum (Prévot 1938) Scholz and Kilian 2016 and Cutibacterium namnetense (Aubin et al. 2016) Nouioui et al. 2018	1670:1823	On the basis of further genomic analysis, we also provide emended descriptions of Cutibacterium granulosum (Prévot 1938) Scholz and Kilian 2016 and Cutibacterium namnetense (Aubin et al. 2016) Nouioui et al. 2018.
32559834	8	38	theme	abundant	1064:1071	arg1	 0					1112:1113	 0	1112:1113	 0	1112:1113	The most abundant major cellular fatty acid was iso-C15 : 0.
32559834	8	38	theme	abundant	1064:1071	arg1	acid					1094:1097	The most abundant major cellular fatty acid	1055:1097	The most abundant major cellular fatty acid	1055:1097	The most abundant major cellular fatty acid was iso-C15 : 0.
32559834	16	39	dep	Kilian	1744:1749	arg1	Aubin					1786:1790	Aubin	1786:1790	Aubin	1786:1790	On the basis of further genomic analysis, we also provide emended descriptions of Cutibacterium granulosum (Prévot 1938) Scholz and Kilian 2016 and Cutibacterium namnetense (Aubin et al. 2016) Nouioui et al. 2018.
32559834	6	40	theme	species	734:740	arg1	strains					699:705	type strains	694:705	type strains of the other Cutibacterium species	694:740	Genome analysis of the strain with type strains of the other Cutibacterium species resulted in digital DNA-DNA hybridization values of 32.3-22.3% and average nucleotide identity (OrthoANI) values of 86.7-73.6 %.
32559834	8	41	theme	cellular	1079:1086	arg1	 0					1112:1113	 0	1112:1113	 0	1112:1113	The most abundant major cellular fatty acid was iso-C15 : 0.
32559834	8	41	theme	cellular	1079:1086	arg1	acid					1094:1097	The most abundant major cellular fatty acid	1055:1097	The most abundant major cellular fatty acid	1055:1097	The most abundant major cellular fatty acid was iso-C15 : 0.
32559834	6	42	theme	identity	828:835	arg1	values					848:853	average nucleotide identity (OrthoANI) values	809:853	average nucleotide identity (OrthoANI) values of 86.7-73.6 %	809:868	Genome analysis of the strain with type strains of the other Cutibacterium species resulted in digital DNA-DNA hybridization values of 32.3-22.3% and average nucleotide identity (OrthoANI) values of 86.7-73.6 %.
32559834	6	43	with	analysis	666:673	arg1	strains					699:705	type strains	694:705	type strains of the other Cutibacterium species	694:740	Genome analysis of the strain with type strains of the other Cutibacterium species resulted in digital DNA-DNA hybridization values of 32.3-22.3% and average nucleotide identity (OrthoANI) values of 86.7-73.6 %.
32559834	2	44	theme	strain	205:210	arg1	bacterium					194:202	An anaerobic and aerotolerant bacterium	164:202	An anaerobic and aerotolerant bacterium	164:202	An anaerobic and aerotolerant bacterium, strain M12T, was isolated from the meibum of inflamed human meibomian glands.
32559834	2	44	theme	strain	205:210	arg1	M12T					212:215	strain M12T	205:215	strain M12T	205:215	An anaerobic and aerotolerant bacterium, strain M12T, was isolated from the meibum of inflamed human meibomian glands.
32559834	6	45	theme	other	714:718	arg1	species					734:740	the other Cutibacterium species	710:740	the other Cutibacterium species	710:740	Genome analysis of the strain with type strains of the other Cutibacterium species resulted in digital DNA-DNA hybridization values of 32.3-22.3% and average nucleotide identity (OrthoANI) values of 86.7-73.6 %.
32559834	6	46	theme	average	809:815	arg1	OrthoANI					838:845	OrthoANI	838:845	OrthoANI	838:845	Genome analysis of the strain with type strains of the other Cutibacterium species resulted in digital DNA-DNA hybridization values of 32.3-22.3% and average nucleotide identity (OrthoANI) values of 86.7-73.6 %.
32559834	6	46	theme	average	809:815	arg1	identity					828:835	average nucleotide identity	809:835	average nucleotide identity (OrthoANI) values of 86.7-73.6 %	809:868	Genome analysis of the strain with type strains of the other Cutibacterium species resulted in digital DNA-DNA hybridization values of 32.3-22.3% and average nucleotide identity (OrthoANI) values of 86.7-73.6 %.
32559834	5	47	theme	closest	621:627	arg1	species					629:635	the closest species	617:635	the closest species	617:635	The 16S rRNA gene sequence of the strain revealed that it belongs to the genus Cutibacterium with a 98.0 % similarity value to the closest species, Cutibacterium acnes.
32559834	6	48	theme	type	694:697	arg1	strains					699:705	type strains	694:705	type strains of the other Cutibacterium species	694:740	Genome analysis of the strain with type strains of the other Cutibacterium species resulted in digital DNA-DNA hybridization values of 32.3-22.3% and average nucleotide identity (OrthoANI) values of 86.7-73.6 %.
32559834	16	49	dep	Cutibacterium	1694:1706	arg1	Nouioui					1805:1811	Nouioui	1805:1811	Nouioui	1805:1811	On the basis of further genomic analysis, we also provide emended descriptions of Cutibacterium granulosum (Prévot 1938) Scholz and Kilian 2016 and Cutibacterium namnetense (Aubin et al. 2016) Nouioui et al. 2018.
32559834	16	49	dep	Cutibacterium	1694:1706	arg1	Scholz					1733:1738	Scholz	1733:1738	Scholz	1733:1738	On the basis of further genomic analysis, we also provide emended descriptions of Cutibacterium granulosum (Prévot 1938) Scholz and Kilian 2016 and Cutibacterium namnetense (Aubin et al. 2016) Nouioui et al. 2018.
32559834	16	49	dep	Cutibacterium	1694:1706	arg1	granulosum					1708:1717	granulosum	1708:1717	Cutibacterium granulosum (Prévot 1938) Scholz	1694:1738	On the basis of further genomic analysis, we also provide emended descriptions of Cutibacterium granulosum (Prévot 1938) Scholz and Kilian 2016 and Cutibacterium namnetense (Aubin et al. 2016) Nouioui et al. 2018.
32559834	16	50	theme	genomic	1636:1642	arg1	analysis					1644:1651	further genomic analysis	1628:1651	further genomic analysis	1628:1651	On the basis of further genomic analysis, we also provide emended descriptions of Cutibacterium granulosum (Prévot 1938) Scholz and Kilian 2016 and Cutibacterium namnetense (Aubin et al. 2016) Nouioui et al. 2018.
32559834	12	51	theme	mass	1369:1372	arg1	spectrum					1387:1394	the MALDI-TOF mass spectrometry spectrum	1355:1394	the MALDI-TOF mass spectrometry spectrum	1355:1394	The major peaks of the MALDI-TOF mass spectrometry spectrum were at 3493, 3712, 6986 and 7424 Da.
32559834	12	52	theme	major	1340:1344	arg1	peaks					1346:1350	The major peaks	1336:1350	The major peaks of the MALDI-TOF mass spectrometry spectrum	1336:1394	The major peaks of the MALDI-TOF mass spectrometry spectrum were at 3493, 3712, 6986 and 7424 Da.
32559834	5	53	theme	similarity	597:606	arg1	value					608:612	a 98.0 % similarity value	588:612	a 98.0 % similarity value	588:612	The 16S rRNA gene sequence of the strain revealed that it belongs to the genus Cutibacterium with a 98.0 % similarity value to the closest species, Cutibacterium acnes.
32559834	15	54	theme	=JCM	1586:1589	arg1	M12T					1580:1583	M12T	1580:1583	M12T (=JCM 33380T=DSM 109769T)	1580:1609	The type strain of C. modestum is M12T (=JCM 33380T=DSM 109769T).
32559834	15	54	theme	=JCM	1586:1589	arg1	109769T					1602:1608	=JCM 33380T=DSM 109769T	1586:1608	=JCM 33380T=DSM 109769T	1586:1608	The type strain of C. modestum is M12T (=JCM 33380T=DSM 109769T).
32559834	2	55	theme	meibomian	265:273	arg1	glands					275:280	inflamed human meibomian glands	250:280	inflamed human meibomian glands	250:280	An anaerobic and aerotolerant bacterium, strain M12T, was isolated from the meibum of inflamed human meibomian glands.
32559834	5	56	theme	16S	494:496	arg1	rRNA					498:501	16S rRNA	494:501	The 16S rRNA gene sequence of the strain	490:529	The 16S rRNA gene sequence of the strain revealed that it belongs to the genus Cutibacterium with a 98.0 % similarity value to the closest species, Cutibacterium acnes.
32559834	12	57	theme	spectrum	1387:1394	arg1	peaks					1346:1350	The major peaks	1336:1350	The major peaks of the MALDI-TOF mass spectrometry spectrum	1336:1394	The major peaks of the MALDI-TOF mass spectrometry spectrum were at 3493, 3712, 6986 and 7424 Da.
32559834	9	58	from	glucose	1147:1153	arg1	end-products					1129:1140	Fermentation end-products	1116:1140	Fermentation end-products from glucose	1116:1153	Fermentation end-products from glucose were propionate, lactate, succinate and acetate.
32559834	6	59	theme	DNA-DNA	762:768	arg1	hybridization					770:782	digital DNA-DNA hybridization	754:782	digital DNA-DNA hybridization values of 32.3-22.3%	754:803	Genome analysis of the strain with type strains of the other Cutibacterium species resulted in digital DNA-DNA hybridization values of 32.3-22.3% and average nucleotide identity (OrthoANI) values of 86.7-73.6 %.
32559834	5	60	theme	gene	503:506	arg1	sequence					508:515	The 16S rRNA gene sequence	490:515	The 16S rRNA gene sequence of the strain	490:529	The 16S rRNA gene sequence of the strain revealed that it belongs to the genus Cutibacterium with a 98.0 % similarity value to the closest species, Cutibacterium acnes.
32559834	4	61	theme	soy	388:390	arg1	plates					397:402	trypticase soy agar plates	377:402	trypticase soy agar plates supplemented with 5 % sheep blood	377:436	Growth on trypticase soy agar plates supplemented with 5 % sheep blood was fastest at 30-37 °C under anaerobic conditions.
32559834	4	62	from	30-37 °C	453:460	arg1	fastest					442:448	fastest	442:448	fastest	442:448	Growth on trypticase soy agar plates supplemented with 5 % sheep blood was fastest at 30-37 °C under anaerobic conditions.
32559834	15	63	theme	modestum	1568:1575	arg1	M12T					1580:1583	M12T	1580:1583	M12T (=JCM 33380T=DSM 109769T)	1580:1609	The type strain of C. modestum is M12T (=JCM 33380T=DSM 109769T).
32559834	15	63	theme	modestum	1568:1575	arg1	strain					1555:1560	The type strain	1546:1560	The type strain of C. modestum	1546:1575	The type strain of C. modestum is M12T (=JCM 33380T=DSM 109769T).
32559834	4	64	from	Growth	367:372	arg1	plates					397:402	trypticase soy agar plates	377:402	trypticase soy agar plates supplemented with 5 % sheep blood	377:436	Growth on trypticase soy agar plates supplemented with 5 % sheep blood was fastest at 30-37 °C under anaerobic conditions.
32559834	7	65	theme	strain	995:1000	arg1	reactivity					977:986	relatively low reactivity	962:986	relatively low reactivity of the strain	962:1000	Biochemical and physiological analyses using API rapid ID 32A and API Coryne kits revealed relatively low reactivity of the strain compared with C. acnes and Cutibacterium namnetense.
32559834	3	66	theme	strain	296:301	arg1	Cells					283:287	Cells	283:287	Cells of the strain	283:301	Cells of the strain was Gram-stain-positive, non-spore-forming and non-motile rods.
32559834	4	67	theme	%	424:424	arg1	blood					432:436	5 % sheep blood	422:436	5 % sheep blood	422:436	Growth on trypticase soy agar plates supplemented with 5 % sheep blood was fastest at 30-37 °C under anaerobic conditions.
32559834	10	68	theme	diamino	1219:1225	arg1	acid					1277:1280	meso-diaminopimelic acid	1257:1280	meso-diaminopimelic acid	1257:1280	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
32559834	10	68	theme	diamino	1219:1225	arg1	acid					1227:1230	The diagnostic diamino acid	1204:1230	The diagnostic diamino acid of the peptidoglycan	1204:1251	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
32559834	2	69	theme	inflamed	250:257	arg1	glands					275:280	inflamed human meibomian glands	250:280	inflamed human meibomian glands	250:280	An anaerobic and aerotolerant bacterium, strain M12T, was isolated from the meibum of inflamed human meibomian glands.
32559834	1	70	theme	Cutibacterium	109:121	arg1	granulosum					123:132	Cutibacterium granulosum	109:132	Cutibacterium granulosum	109:132	nov., isolated from meibum of human meibomian glands, and emended descriptions of Cutibacterium granulosum and Cutibacterium namnetense.
32559834	15	71	theme	type	1550:1553	arg1	M12T					1580:1583	M12T	1580:1583	M12T (=JCM 33380T=DSM 109769T)	1580:1609	The type strain of C. modestum is M12T (=JCM 33380T=DSM 109769T).
32559834	15	71	theme	type	1550:1553	arg1	strain					1555:1560	The type strain	1546:1560	The type strain of C. modestum	1546:1575	The type strain of C. modestum is M12T (=JCM 33380T=DSM 109769T).
32559834	7	72	theme	low	973:975	arg1	reactivity					977:986	relatively low reactivity	962:986	relatively low reactivity of the strain	962:1000	Biochemical and physiological analyses using API rapid ID 32A and API Coryne kits revealed relatively low reactivity of the strain compared with C. acnes and Cutibacterium namnetense.
32559834	3	73	theme	non-spore-forming	328:344	arg1	rods					361:364	Gram-stain-positive, non-spore-forming and non-motile rods	307:364	Gram-stain-positive, non-spore-forming and non-motile rods	307:364	Cells of the strain was Gram-stain-positive, non-spore-forming and non-motile rods.
32559834	10	74	theme	peptidoglycan	1239:1251	arg1	acid					1277:1280	meso-diaminopimelic acid	1257:1280	meso-diaminopimelic acid	1257:1280	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
32559834	10	74	theme	peptidoglycan	1239:1251	arg1	acid					1227:1230	The diagnostic diamino acid	1204:1230	The diagnostic diamino acid of the peptidoglycan	1204:1251	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
32559834	5	75	theme	strain	524:529	arg1	sequence					508:515	The 16S rRNA gene sequence	490:515	The 16S rRNA gene sequence of the strain	490:529	The 16S rRNA gene sequence of the strain revealed that it belongs to the genus Cutibacterium with a 98.0 % similarity value to the closest species, Cutibacterium acnes.
32559834	2	76	attach	isolated	222:229	arg2	M12T					212:215	strain M12T	205:215	strain M12T	205:215	An anaerobic and aerotolerant bacterium, strain M12T, was isolated from the meibum of inflamed human meibomian glands.
32559834	2	76	attach	isolated	222:229	arg1	meibum					240:245	the meibum	236:245	the meibum of inflamed human meibomian glands	236:280	An anaerobic and aerotolerant bacterium, strain M12T, was isolated from the meibum of inflamed human meibomian glands.
32559834	2	76	attach	isolated	222:229	arg2	bacterium					194:202	An anaerobic and aerotolerant bacterium	164:202	An anaerobic and aerotolerant bacterium	164:202	An anaerobic and aerotolerant bacterium, strain M12T, was isolated from the meibum of inflamed human meibomian glands.
32559834	3	77	theme	Gram-stain-positive	307:325	arg1	rods					361:364	Gram-stain-positive, non-spore-forming and non-motile rods	307:364	Gram-stain-positive, non-spore-forming and non-motile rods	307:364	Cells of the strain was Gram-stain-positive, non-spore-forming and non-motile rods.
32559834	7	78	theme	Coryne	941:946	arg1	kits					948:951	API rapid ID 32A and API Coryne kits	916:951	API rapid ID 32A and API Coryne kits	916:951	Biochemical and physiological analyses using API rapid ID 32A and API Coryne kits revealed relatively low reactivity of the strain compared with C. acnes and Cutibacterium namnetense.
32559834	4	79	theme	anaerobic	468:476	arg1	conditions					478:487	anaerobic conditions	468:487	anaerobic conditions	468:487	Growth on trypticase soy agar plates supplemented with 5 % sheep blood was fastest at 30-37 °C under anaerobic conditions.
32559834	1	80	attach	isolated	33:40	arg1	meibum					47:52	meibum	47:52	meibum of human meibomian glands	47:78	nov., isolated from meibum of human meibomian glands, and emended descriptions of Cutibacterium granulosum and Cutibacterium namnetense.
32559834	1	80	attach	isolated	33:40	arg2	nov.					27:30	nov.	27:30	nov.	27:30	nov., isolated from meibum of human meibomian glands, and emended descriptions of Cutibacterium granulosum and Cutibacterium namnetense.
32559834	5	81	theme	%	595:595	arg1	similarity					597:606	a 98.0 % similarity	588:606	a 98.0 % similarity value	588:612	The 16S rRNA gene sequence of the strain revealed that it belongs to the genus Cutibacterium with a 98.0 % similarity value to the closest species, Cutibacterium acnes.
32559834	1	82	theme	human	57:61	arg1	glands					73:78	human meibomian glands	57:78	human meibomian glands	57:78	nov., isolated from meibum of human meibomian glands, and emended descriptions of Cutibacterium granulosum and Cutibacterium namnetense.
32559834	3	83	theme	non-motile	350:359	arg1	rods					361:364	Gram-stain-positive, non-spore-forming and non-motile rods	307:364	Gram-stain-positive, non-spore-forming and non-motile rods	307:364	Cells of the strain was Gram-stain-positive, non-spore-forming and non-motile rods.
32559834	10	84	theme	meso-diaminopimelic	1257:1275	arg1	acid					1277:1280	meso-diaminopimelic acid	1257:1280	meso-diaminopimelic acid	1257:1280	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
32559834	10	84	theme	meso-diaminopimelic	1257:1275	arg1	acid					1227:1230	The diagnostic diamino acid	1204:1230	The diagnostic diamino acid of the peptidoglycan	1204:1251	The diagnostic diamino acid of the peptidoglycan was meso-diaminopimelic acid.
32559834	0	85	theme	modestum	14:21	arg1	sp					23:24	Cutibacterium modestum sp	0:24	Cutibacterium modestum sp.	0:25	Cutibacterium modestum sp.
32559834	7	86	theme	ID	926:927	arg1	32A					929:931	API rapid ID 32A	916:931	API rapid ID 32A	916:931	Biochemical and physiological analyses using API rapid ID 32A and API Coryne kits revealed relatively low reactivity of the strain compared with C. acnes and Cutibacterium namnetense.
32559834	1	87	theme	glands	73:78	arg1	meibum					47:52	meibum	47:52	meibum of human meibomian glands	47:78	nov., isolated from meibum of human meibomian glands, and emended descriptions of Cutibacterium granulosum and Cutibacterium namnetense.
32559834	13	88	theme	DNA	1438:1440	arg1	content					1446:1452	The DNA G+C content	1434:1452	The DNA G+C content	1434:1452	The DNA G+C content was 59.9 mol%.
32559834	13	88	theme	DNA	1438:1440	arg1	%					1466:1466	59.9 mol%	1458:1466	59.9 mol%	1458:1466	The DNA G+C content was 59.9 mol%.
32559834	7	89	theme	API	916:918	arg1	32A					929:931	API rapid ID 32A	916:931	API rapid ID 32A	916:931	Biochemical and physiological analyses using API rapid ID 32A and API Coryne kits revealed relatively low reactivity of the strain compared with C. acnes and Cutibacterium namnetense.
32559834	2	90	theme	aerotolerant	181:192	arg1	bacterium					194:202	An anaerobic and aerotolerant bacterium	164:202	An anaerobic and aerotolerant bacterium	164:202	An anaerobic and aerotolerant bacterium, strain M12T, was isolated from the meibum of inflamed human meibomian glands.
32559834	2	90	theme	aerotolerant	181:192	arg1	M12T					212:215	strain M12T	205:215	strain M12T	205:215	An anaerobic and aerotolerant bacterium, strain M12T, was isolated from the meibum of inflamed human meibomian glands.
32559834	8	91	theme	major	1073:1077	arg1	 0					1112:1113	 0	1112:1113	 0	1112:1113	The most abundant major cellular fatty acid was iso-C15 : 0.
32559834	8	91	theme	major	1073:1077	arg1	acid					1094:1097	The most abundant major cellular fatty acid	1055:1097	The most abundant major cellular fatty acid	1055:1097	The most abundant major cellular fatty acid was iso-C15 : 0.
32559834	2	92	theme	anaerobic	167:175	arg1	bacterium					194:202	An anaerobic and aerotolerant bacterium	164:202	An anaerobic and aerotolerant bacterium	164:202	An anaerobic and aerotolerant bacterium, strain M12T, was isolated from the meibum of inflamed human meibomian glands.
32559834	2	92	theme	anaerobic	167:175	arg1	M12T					212:215	strain M12T	205:215	strain M12T	205:215	An anaerobic and aerotolerant bacterium, strain M12T, was isolated from the meibum of inflamed human meibomian glands.
32559834	8	93	theme	fatty	1088:1092	arg1	 0					1112:1113	 0	1112:1113	 0	1112:1113	The most abundant major cellular fatty acid was iso-C15 : 0.
32559834	8	93	theme	fatty	1088:1092	arg1	acid					1094:1097	The most abundant major cellular fatty acid	1055:1097	The most abundant major cellular fatty acid	1055:1097	The most abundant major cellular fatty acid was iso-C15 : 0.
32559834	6	94	theme	Cutibacterium	720:732	arg1	species					734:740	the other Cutibacterium species	710:740	the other Cutibacterium species	710:740	Genome analysis of the strain with type strains of the other Cutibacterium species resulted in digital DNA-DNA hybridization values of 32.3-22.3% and average nucleotide identity (OrthoANI) values of 86.7-73.6 %.
32559834	7	95	theme	physiological	887:899	arg1	analyses					901:908	Biochemical and physiological analyses	871:908	Biochemical and physiological analyses using API rapid ID 32A and API Coryne kits	871:951	Biochemical and physiological analyses using API rapid ID 32A and API Coryne kits revealed relatively low reactivity of the strain compared with C. acnes and Cutibacterium namnetense.
32559834	6	96	theme	nucleotide	817:826	arg1	OrthoANI					838:845	OrthoANI	838:845	OrthoANI	838:845	Genome analysis of the strain with type strains of the other Cutibacterium species resulted in digital DNA-DNA hybridization values of 32.3-22.3% and average nucleotide identity (OrthoANI) values of 86.7-73.6 %.
32559834	6	96	theme	nucleotide	817:826	arg1	identity					828:835	average nucleotide identity	809:835	average nucleotide identity (OrthoANI) values of 86.7-73.6 %	809:868	Genome analysis of the strain with type strains of the other Cutibacterium species resulted in digital DNA-DNA hybridization values of 32.3-22.3% and average nucleotide identity (OrthoANI) values of 86.7-73.6 %.
32559834	9	97	theme	Fermentation	1116:1127	arg1	end-products					1129:1140	Fermentation end-products	1116:1140	Fermentation end-products from glucose	1116:1153	Fermentation end-products from glucose were propionate, lactate, succinate and acetate.
32559834	7	98	theme	Biochemical	871:881	arg1	analyses					901:908	Biochemical and physiological analyses	871:908	Biochemical and physiological analyses using API rapid ID 32A and API Coryne kits	871:951	Biochemical and physiological analyses using API rapid ID 32A and API Coryne kits revealed relatively low reactivity of the strain compared with C. acnes and Cutibacterium namnetense.
33325979	1	0	from	microparticles	425:438	arg1	Zn					363:364	Zn	363:364	Zn	363:364	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	0	from	microparticles	425:438	arg1	microparticles					289:302	hydroxyapatite/chondroitin sulfate composite microparticles	244:302	hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS)	244:312	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	0	from	microparticles	425:438	arg1	carrier					180:186	a local delivery carrier	163:186	a local delivery carrier of bone metabolic proteins	163:213	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	0	from	microparticles	425:438	arg1	properties					393:402	the in vitro release properties	372:402	the in vitro release properties of proteins from the microparticles	372:438	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	0	from	microparticles	425:438	arg1	method					336:341	their formulation method	318:341	their formulation method using zinc cations (Zn)	318:365	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	0	from	microparticles	425:438	arg1	HAp/ChS					305:311	HAp/ChS	305:311	HAp/ChS	305:311	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	0	from	microparticles	425:438	arg1	proteins					407:414	proteins	407:414	proteins from the microparticles	407:438	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	3	1	theme	antibodies	710:719	arg1	release					699:705	the release	695:705	the release of antibodies from HAp/ChS in phosphate buffer saline	695:759	Adding Zn in the formulation of IgG loaded with HAp/ChS microparticles enhanced the release of antibodies from HAp/ChS in phosphate buffer saline.
33325979	7	2	from	hole	1172:1175	arg1	bone					1199:1202	the distal femoral bone	1180:1202	the distal femoral bone of young rats	1180:1216	We prepared HAp/ChS microparticles loaded with Sclmab and locally administered the microparticles into a drilled hole in the distal femoral bone of young rats.
33325979	0	3	theme	hydroxyapatite/chondroitin	76:101	arg1	sulfate					103:109	hydroxyapatite/chondroitin sulfate	76:109	hydroxyapatite/chondroitin sulfate	76:109	Local administration and enhanced release of bone metabolic antibodies from hydroxyapatite/chondroitin sulfate nanocomposite microparticles using zinc cations.
33325979	8	4	theme	control	1415:1421	arg1	group					1423:1427	the control group	1411:1427	the control group (normal saline)	1411:1443	After three weeks, the area of the newly formed osteoid around the drilled hole where HAp/ChS loaded with Sclmab and Zn was locally administered was significantly higher than that observed in the control group (normal saline).
33325979	8	4	theme	control	1415:1421	arg1	saline					1437:1442	normal saline	1430:1442	normal saline	1430:1442	After three weeks, the area of the newly formed osteoid around the drilled hole where HAp/ChS loaded with Sclmab and Zn was locally administered was significantly higher than that observed in the control group (normal saline).
33325979	5	5	from	effect	939:944	arg1	treatment					953:961	the treatment	949:961	the treatment for local bone diseases	949:985	These results suggest a possible beneficial effect on the treatment for local bone diseases.
33325979	2	6	theme	human	532:536	arg1	hIgG					543:546	hIgG	543:546	hIgG	543:546	Herein, we report the release properties of model antibodies such as immunoglobulin (IgG), human IgG (hIgG), and denosumab (Dmab) from HAp/ChS using this formulation method.
33325979	2	6	theme	human	532:536	arg1	IgG					538:540	human IgG	532:540	human IgG (hIgG)	532:547	Herein, we report the release properties of model antibodies such as immunoglobulin (IgG), human IgG (hIgG), and denosumab (Dmab) from HAp/ChS using this formulation method.
33325979	9	7	theme	local	1572:1576	arg1	diseases					1583:1590	local bone diseases	1572:1590	local bone diseases	1572:1590	Thus, HAp/ChS microparticles and the formulation method of monoclonal antibodies using Zn could be useful in the treatment of local bone diseases.
33325979	7	8	theme	femoral	1191:1197	arg1	bone					1199:1202	the distal femoral bone	1180:1202	the distal femoral bone of young rats	1180:1216	We prepared HAp/ChS microparticles loaded with Sclmab and locally administered the microparticles into a drilled hole in the distal femoral bone of young rats.
33325979	1	9	theme	in	376:377	arg1	method					336:341	their formulation method	318:341	their formulation method using zinc cations (Zn)	318:365	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	9	theme	in	376:377	arg1	microparticles					289:302	hydroxyapatite/chondroitin sulfate composite microparticles	244:302	hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS)	244:312	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	9	theme	in	376:377	arg1	carrier					180:186	a local delivery carrier	163:186	a local delivery carrier of bone metabolic proteins	163:213	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	9	theme	in	376:377	arg1	properties					393:402	the in vitro release properties	372:402	the in vitro release properties of proteins from the microparticles	372:438	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	9	10	from	useful	1545:1550	arg1	treatment					1559:1567	the treatment	1555:1567	the treatment of local bone diseases	1555:1590	Thus, HAp/ChS microparticles and the formulation method of monoclonal antibodies using Zn could be useful in the treatment of local bone diseases.
33325979	9	11	theme	HAp/ChS	1452:1458	arg1	microparticles					1460:1473	HAp/ChS microparticles	1452:1473	HAp/ChS microparticles	1452:1473	Thus, HAp/ChS microparticles and the formulation method of monoclonal antibodies using Zn could be useful in the treatment of local bone diseases.
33325979	0	12	theme	zinc	146:149	arg1	cations					151:157	zinc cations	146:157	zinc cations	146:157	Local administration and enhanced release of bone metabolic antibodies from hydroxyapatite/chondroitin sulfate nanocomposite microparticles using zinc cations.
33325979	1	13	from	method	336:341	arg1	microparticles					425:438	the microparticles	421:438	the microparticles	421:438	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	6	14	theme	monoclonal	1003:1012	arg1	Sclmab					1024:1029	Sclmab	1024:1029	Sclmab	1024:1029	The sclerostin monoclonal antibody (Sclmab) promotes fracture healing.
33325979	6	14	theme	monoclonal	1003:1012	arg1	antibody					1014:1021	monoclonal antibody	1003:1021	The sclerostin monoclonal antibody (Sclmab)	988:1030	The sclerostin monoclonal antibody (Sclmab) promotes fracture healing.
33325979	6	15	theme	fracture	1041:1048	arg1	healing					1050:1056	fracture healing	1041:1056	fracture healing	1041:1056	The sclerostin monoclonal antibody (Sclmab) promotes fracture healing.
33325979	1	16	theme	bone	191:194	arg1	proteins					206:213	bone metabolic proteins	191:213	bone metabolic proteins	191:213	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	8	17	located	observed	1399:1406	arg2	that					1394:1397	that	1394:1397	that	1394:1397	After three weeks, the area of the newly formed osteoid around the drilled hole where HAp/ChS loaded with Sclmab and Zn was locally administered was significantly higher than that observed in the control group (normal saline).
33325979	8	17	located	observed	1399:1406	arg1	group					1423:1427	the control group	1411:1427	the control group (normal saline)	1411:1443	After three weeks, the area of the newly formed osteoid around the drilled hole where HAp/ChS loaded with Sclmab and Zn was locally administered was significantly higher than that observed in the control group (normal saline).
33325979	8	17	located	observed	1399:1406	arg1	saline					1437:1442	normal saline	1430:1442	normal saline	1430:1442	After three weeks, the area of the newly formed osteoid around the drilled hole where HAp/ChS loaded with Sclmab and Zn was locally administered was significantly higher than that observed in the control group (normal saline).
33325979	1	18	theme	release	385:391	arg1	method					336:341	their formulation method	318:341	their formulation method using zinc cations (Zn)	318:365	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	18	theme	release	385:391	arg1	microparticles					289:302	hydroxyapatite/chondroitin sulfate composite microparticles	244:302	hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS)	244:312	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	18	theme	release	385:391	arg1	carrier					180:186	a local delivery carrier	163:186	a local delivery carrier of bone metabolic proteins	163:213	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	18	theme	release	385:391	arg1	properties					393:402	the in vitro release properties	372:402	the in vitro release properties of proteins from the microparticles	372:438	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	2	19	theme	formulation	595:605	arg1	method					607:612	this formulation method	590:612	this formulation method	590:612	Herein, we report the release properties of model antibodies such as immunoglobulin (IgG), human IgG (hIgG), and denosumab (Dmab) from HAp/ChS using this formulation method.
33325979	1	20	from	properties	393:402	arg1	microparticles					425:438	the microparticles	421:438	the microparticles	421:438	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	21	theme	metabolic	196:204	arg1	proteins					206:213	bone metabolic proteins	191:213	bone metabolic proteins	191:213	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	2	22	theme	release	463:469	arg1	immunoglobulin					510:523	immunoglobulin	510:523	immunoglobulin (IgG)	510:529	Herein, we report the release properties of model antibodies such as immunoglobulin (IgG), human IgG (hIgG), and denosumab (Dmab) from HAp/ChS using this formulation method.
33325979	2	22	theme	release	463:469	arg1	IgG					538:540	human IgG	532:540	human IgG (hIgG)	532:547	Herein, we report the release properties of model antibodies such as immunoglobulin (IgG), human IgG (hIgG), and denosumab (Dmab) from HAp/ChS using this formulation method.
33325979	2	22	theme	release	463:469	arg1	denosumab					554:562	denosumab	554:562	denosumab (Dmab)	554:569	Herein, we report the release properties of model antibodies such as immunoglobulin (IgG), human IgG (hIgG), and denosumab (Dmab) from HAp/ChS using this formulation method.
33325979	2	22	theme	release	463:469	arg1	properties					471:480	the release properties	459:480	the release properties of model antibodies such as immunoglobulin (IgG), human IgG (hIgG), and denosumab (Dmab) from HAp/ChS using this formulation method	459:612	Herein, we report the release properties of model antibodies such as immunoglobulin (IgG), human IgG (hIgG), and denosumab (Dmab) from HAp/ChS using this formulation method.
33325979	6	23	dep	sclerostin	992:1001	arg1	Sclmab					1024:1029	Sclmab	1024:1029	Sclmab	1024:1029	The sclerostin monoclonal antibody (Sclmab) promotes fracture healing.
33325979	6	23	dep	sclerostin	992:1001	arg1	antibody					1014:1021	monoclonal antibody	1003:1021	The sclerostin monoclonal antibody (Sclmab)	988:1030	The sclerostin monoclonal antibody (Sclmab) promotes fracture healing.
33325979	3	24	from	HAp/ChS	726:732	arg1	saline					754:759	phosphate buffer saline	737:759	phosphate buffer saline	737:759	Adding Zn in the formulation of IgG loaded with HAp/ChS microparticles enhanced the release of antibodies from HAp/ChS in phosphate buffer saline.
33325979	3	24	from	HAp/ChS	726:732	arg1	release					699:705	the release	695:705	the release of antibodies from HAp/ChS in phosphate buffer saline	695:759	Adding Zn in the formulation of IgG loaded with HAp/ChS microparticles enhanced the release of antibodies from HAp/ChS in phosphate buffer saline.
33325979	7	25	theme	drilled	1164:1170	arg1	hole					1172:1175	a drilled hole	1162:1175	a drilled hole in the distal femoral bone of young rats	1162:1216	We prepared HAp/ChS microparticles loaded with Sclmab and locally administered the microparticles into a drilled hole in the distal femoral bone of young rats.
33325979	1	26	theme	proteins	206:213	arg1	method					336:341	their formulation method	318:341	their formulation method using zinc cations (Zn)	318:365	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	26	theme	proteins	206:213	arg1	microparticles					289:302	hydroxyapatite/chondroitin sulfate composite microparticles	244:302	hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS)	244:312	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	26	theme	proteins	206:213	arg1	carrier					180:186	a local delivery carrier	163:186	a local delivery carrier of bone metabolic proteins	163:213	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	26	theme	proteins	206:213	arg1	properties					393:402	the in vitro release properties	372:402	the in vitro release properties of proteins from the microparticles	372:438	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	0	27	theme	Local	0:4	arg1	administration					6:19	Local administration	0:19	Local administration	0:19	Local administration and enhanced release of bone metabolic antibodies from hydroxyapatite/chondroitin sulfate nanocomposite microparticles using zinc cations.
33325979	7	28	theme	HAp/ChS	1071:1077	arg1	microparticles					1079:1092	HAp/ChS microparticles	1071:1092	HAp/ChS microparticles loaded with Sclmab	1071:1111	We prepared HAp/ChS microparticles loaded with Sclmab and locally administered the microparticles into a drilled hole in the distal femoral bone of young rats.
33325979	0	29	theme	enhanced	25:32	arg1	release					34:40	enhanced release	25:40	enhanced release of bone metabolic antibodies from hydroxyapatite/chondroitin sulfate	25:109	Local administration and enhanced release of bone metabolic antibodies from hydroxyapatite/chondroitin sulfate nanocomposite microparticles using zinc cations.
33325979	1	30	theme	formulation	324:334	arg1	method					336:341	their formulation method	318:341	their formulation method using zinc cations (Zn)	318:365	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	30	theme	formulation	324:334	arg1	properties					393:402	the in vitro release properties	372:402	the in vitro release properties of proteins from the microparticles	372:438	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	30	theme	formulation	324:334	arg1	carrier					180:186	a local delivery carrier	163:186	a local delivery carrier of bone metabolic proteins	163:213	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	30	theme	formulation	324:334	arg1	microparticles					289:302	hydroxyapatite/chondroitin sulfate composite microparticles	244:302	hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS)	244:312	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	30	theme	formulation	324:334	arg1	Zn					363:364	Zn	363:364	Zn	363:364	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	5	31	theme	possible	919:926	arg1	effect					939:944	a possible beneficial effect	917:944	a possible beneficial effect on the treatment for local bone diseases	917:985	These results suggest a possible beneficial effect on the treatment for local bone diseases.
33325979	9	32	from	treatment	1559:1567	arg1	useful					1545:1550	useful	1545:1550	useful	1545:1550	Thus, HAp/ChS microparticles and the formulation method of monoclonal antibodies using Zn could be useful in the treatment of local bone diseases.
33325979	9	32	from	treatment	1559:1567	arg1	method					1495:1500	the formulation method	1479:1500	the formulation method	1479:1500	Thus, HAp/ChS microparticles and the formulation method of monoclonal antibodies using Zn could be useful in the treatment of local bone diseases.
33325979	2	33	from	HAp/ChS	576:582	arg1	denosumab					554:562	denosumab	554:562	denosumab (Dmab)	554:569	Herein, we report the release properties of model antibodies such as immunoglobulin (IgG), human IgG (hIgG), and denosumab (Dmab) from HAp/ChS using this formulation method.
33325979	2	33	from	HAp/ChS	576:582	arg1	immunoglobulin					510:523	immunoglobulin	510:523	immunoglobulin (IgG)	510:529	Herein, we report the release properties of model antibodies such as immunoglobulin (IgG), human IgG (hIgG), and denosumab (Dmab) from HAp/ChS using this formulation method.
33325979	2	33	from	HAp/ChS	576:582	arg1	IgG					538:540	human IgG	532:540	human IgG (hIgG)	532:547	Herein, we report the release properties of model antibodies such as immunoglobulin (IgG), human IgG (hIgG), and denosumab (Dmab) from HAp/ChS using this formulation method.
33325979	2	33	from	HAp/ChS	576:582	arg1	hIgG					543:546	hIgG	543:546	hIgG	543:546	Herein, we report the release properties of model antibodies such as immunoglobulin (IgG), human IgG (hIgG), and denosumab (Dmab) from HAp/ChS using this formulation method.
33325979	2	33	from	HAp/ChS	576:582	arg1	Dmab					565:568	Dmab	565:568	Dmab	565:568	Herein, we report the release properties of model antibodies such as immunoglobulin (IgG), human IgG (hIgG), and denosumab (Dmab) from HAp/ChS using this formulation method.
33325979	2	33	from	HAp/ChS	576:582	arg1	IgG					526:528	IgG	526:528	IgG	526:528	Herein, we report the release properties of model antibodies such as immunoglobulin (IgG), human IgG (hIgG), and denosumab (Dmab) from HAp/ChS using this formulation method.
33325979	9	34	theme	monoclonal	1505:1514	arg1	antibodies					1516:1525	monoclonal antibodies	1505:1525	monoclonal antibodies using Zn	1505:1534	Thus, HAp/ChS microparticles and the formulation method of monoclonal antibodies using Zn could be useful in the treatment of local bone diseases.
33325979	0	35	from	sulfate	103:109	arg1	administration					6:19	Local administration	0:19	Local administration	0:19	Local administration and enhanced release of bone metabolic antibodies from hydroxyapatite/chondroitin sulfate nanocomposite microparticles using zinc cations.
33325979	0	35	from	sulfate	103:109	arg1	release					34:40	enhanced release	25:40	enhanced release of bone metabolic antibodies from hydroxyapatite/chondroitin sulfate	25:109	Local administration and enhanced release of bone metabolic antibodies from hydroxyapatite/chondroitin sulfate nanocomposite microparticles using zinc cations.
33325979	5	36	theme	beneficial	928:937	arg1	effect					939:944	a possible beneficial effect	917:944	a possible beneficial effect on the treatment for local bone diseases	917:985	These results suggest a possible beneficial effect on the treatment for local bone diseases.
33325979	9	37	theme	formulation	1483:1493	arg1	method					1495:1500	the formulation method	1479:1500	the formulation method	1479:1500	Thus, HAp/ChS microparticles and the formulation method of monoclonal antibodies using Zn could be useful in the treatment of local bone diseases.
33325979	9	37	theme	formulation	1483:1493	arg1	useful					1545:1550	useful	1545:1550	useful	1545:1550	Thus, HAp/ChS microparticles and the formulation method of monoclonal antibodies using Zn could be useful in the treatment of local bone diseases.
33325979	2	38	theme	antibodies	491:500	arg1	immunoglobulin					510:523	immunoglobulin	510:523	immunoglobulin (IgG)	510:529	Herein, we report the release properties of model antibodies such as immunoglobulin (IgG), human IgG (hIgG), and denosumab (Dmab) from HAp/ChS using this formulation method.
33325979	2	38	theme	antibodies	491:500	arg1	IgG					538:540	human IgG	532:540	human IgG (hIgG)	532:547	Herein, we report the release properties of model antibodies such as immunoglobulin (IgG), human IgG (hIgG), and denosumab (Dmab) from HAp/ChS using this formulation method.
33325979	2	38	theme	antibodies	491:500	arg1	denosumab					554:562	denosumab	554:562	denosumab (Dmab)	554:569	Herein, we report the release properties of model antibodies such as immunoglobulin (IgG), human IgG (hIgG), and denosumab (Dmab) from HAp/ChS using this formulation method.
33325979	2	38	theme	antibodies	491:500	arg1	properties					471:480	the release properties	459:480	the release properties of model antibodies such as immunoglobulin (IgG), human IgG (hIgG), and denosumab (Dmab) from HAp/ChS using this formulation method	459:612	Herein, we report the release properties of model antibodies such as immunoglobulin (IgG), human IgG (hIgG), and denosumab (Dmab) from HAp/ChS using this formulation method.
33325979	8	39	theme	drilled	1286:1292	arg1	hole					1294:1297	the drilled hole	1282:1297	the drilled hole where HAp/ChS loaded with Sclmab and Zn was locally administered	1282:1362	After three weeks, the area of the newly formed osteoid around the drilled hole where HAp/ChS loaded with Sclmab and Zn was locally administered was significantly higher than that observed in the control group (normal saline).
33325979	1	40	theme	proteins	407:414	arg1	Zn					363:364	Zn	363:364	Zn	363:364	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	40	theme	proteins	407:414	arg1	microparticles					289:302	hydroxyapatite/chondroitin sulfate composite microparticles	244:302	hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS)	244:312	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	40	theme	proteins	407:414	arg1	carrier					180:186	a local delivery carrier	163:186	a local delivery carrier of bone metabolic proteins	163:213	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	40	theme	proteins	407:414	arg1	properties					393:402	the in vitro release properties	372:402	the in vitro release properties of proteins from the microparticles	372:438	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	40	theme	proteins	407:414	arg1	method					336:341	their formulation method	318:341	their formulation method using zinc cations (Zn)	318:365	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	40	theme	proteins	407:414	arg1	HAp/ChS					305:311	HAp/ChS	305:311	HAp/ChS	305:311	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	3	41	from	Zn	622:623	arg1	formulation					632:642	the formulation	628:642	the formulation of IgG loaded with HAp/ChS microparticles	628:684	Adding Zn in the formulation of IgG loaded with HAp/ChS microparticles enhanced the release of antibodies from HAp/ChS in phosphate buffer saline.
33325979	2	42	theme	model	485:489	arg1	antibodies					491:500	model antibodies	485:500	model antibodies	485:500	Herein, we report the release properties of model antibodies such as immunoglobulin (IgG), human IgG (hIgG), and denosumab (Dmab) from HAp/ChS using this formulation method.
33325979	1	43	theme	zinc	349:352	arg1	cations					354:360	zinc cations	349:360	zinc cations	349:360	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	3	44	theme	phosphate	737:745	arg1	buffer					747:752	phosphate buffer	737:752	phosphate buffer saline	737:759	Adding Zn in the formulation of IgG loaded with HAp/ChS microparticles enhanced the release of antibodies from HAp/ChS in phosphate buffer saline.
33325979	4	45	theme	biological	779:788	arg1	activity					790:797	the biological activity	775:797	the biological activity of Dmab released from HAp/ChS formulated with Zn	775:846	In addition, the biological activity of Dmab released from HAp/ChS formulated with Zn was significantly higher than that without Zn.
33325979	4	45	theme	biological	779:788	arg1	higher					866:871	higher	866:871	higher	866:871	In addition, the biological activity of Dmab released from HAp/ChS formulated with Zn was significantly higher than that without Zn.
33325979	0	46	theme	metabolic	50:58	arg1	antibodies					60:69	bone metabolic antibodies	45:69	bone metabolic antibodies	45:69	Local administration and enhanced release of bone metabolic antibodies from hydroxyapatite/chondroitin sulfate nanocomposite microparticles using zinc cations.
33325979	7	47	theme	distal	1184:1189	arg1	bone					1199:1202	the distal femoral bone	1180:1202	the distal femoral bone of young rats	1180:1216	We prepared HAp/ChS microparticles loaded with Sclmab and locally administered the microparticles into a drilled hole in the distal femoral bone of young rats.
33325979	3	48	theme	Adding	615:620	arg1	Zn					622:623	Zn	622:623	Adding Zn in the formulation of IgG loaded with HAp/ChS microparticles	615:684	Adding Zn in the formulation of IgG loaded with HAp/ChS microparticles enhanced the release of antibodies from HAp/ChS in phosphate buffer saline.
33325979	4	49	theme	Dmab	802:805	arg1	activity					790:797	the biological activity	775:797	the biological activity of Dmab released from HAp/ChS formulated with Zn	775:846	In addition, the biological activity of Dmab released from HAp/ChS formulated with Zn was significantly higher than that without Zn.
33325979	4	49	theme	Dmab	802:805	arg1	higher					866:871	higher	866:871	higher	866:871	In addition, the biological activity of Dmab released from HAp/ChS formulated with Zn was significantly higher than that without Zn.
33325979	0	50	theme	bone	45:48	arg1	antibodies					60:69	bone metabolic antibodies	45:69	bone metabolic antibodies	45:69	Local administration and enhanced release of bone metabolic antibodies from hydroxyapatite/chondroitin sulfate nanocomposite microparticles using zinc cations.
33325979	1	51	dep	in	376:377	arg1	vitro					379:383	vitro	379:383	vitro	379:383	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	52	from	microparticles	289:302	arg1	microparticles					425:438	the microparticles	421:438	the microparticles	421:438	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	8	53	theme	normal	1430:1435	arg1	group					1423:1427	the control group	1411:1427	the control group (normal saline)	1411:1443	After three weeks, the area of the newly formed osteoid around the drilled hole where HAp/ChS loaded with Sclmab and Zn was locally administered was significantly higher than that observed in the control group (normal saline).
33325979	8	53	theme	normal	1430:1435	arg1	saline					1437:1442	normal saline	1430:1442	normal saline	1430:1442	After three weeks, the area of the newly formed osteoid around the drilled hole where HAp/ChS loaded with Sclmab and Zn was locally administered was significantly higher than that observed in the control group (normal saline).
33325979	9	54	theme	bone	1578:1581	arg1	diseases					1583:1590	local bone diseases	1572:1590	local bone diseases	1572:1590	Thus, HAp/ChS microparticles and the formulation method of monoclonal antibodies using Zn could be useful in the treatment of local bone diseases.
33325979	3	55	theme	buffer	747:752	arg1	saline					754:759	phosphate buffer saline	737:759	phosphate buffer saline	737:759	Adding Zn in the formulation of IgG loaded with HAp/ChS microparticles enhanced the release of antibodies from HAp/ChS in phosphate buffer saline.
33325979	3	56	theme	IgG	647:649	arg1	formulation					632:642	the formulation	628:642	the formulation of IgG loaded with HAp/ChS microparticles	628:684	Adding Zn in the formulation of IgG loaded with HAp/ChS microparticles enhanced the release of antibodies from HAp/ChS in phosphate buffer saline.
33325979	8	57	theme	osteoid	1267:1273	arg1	area					1242:1245	the area	1238:1245	the area of the newly formed osteoid around the drilled hole where HAp/ChS loaded with Sclmab and Zn was locally administered	1238:1362	After three weeks, the area of the newly formed osteoid around the drilled hole where HAp/ChS loaded with Sclmab and Zn was locally administered was significantly higher than that observed in the control group (normal saline).
33325979	8	57	theme	osteoid	1267:1273	arg1	higher					1382:1387	higher	1382:1387	higher	1382:1387	After three weeks, the area of the newly formed osteoid around the drilled hole where HAp/ChS loaded with Sclmab and Zn was locally administered was significantly higher than that observed in the control group (normal saline).
33325979	1	58	theme	hydroxyapatite/chondroitin	244:269	arg1	method					336:341	their formulation method	318:341	their formulation method using zinc cations (Zn)	318:365	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	58	theme	hydroxyapatite/chondroitin	244:269	arg1	HAp/ChS					305:311	HAp/ChS	305:311	HAp/ChS	305:311	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	58	theme	hydroxyapatite/chondroitin	244:269	arg1	microparticles					289:302	hydroxyapatite/chondroitin sulfate composite microparticles	244:302	hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS)	244:312	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	58	theme	hydroxyapatite/chondroitin	244:269	arg1	carrier					180:186	a local delivery carrier	163:186	a local delivery carrier of bone metabolic proteins	163:213	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	58	theme	hydroxyapatite/chondroitin	244:269	arg1	properties					393:402	the in vitro release properties	372:402	the in vitro release properties of proteins from the microparticles	372:438	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	9	59	theme	antibodies	1516:1525	arg1	method					1495:1500	the formulation method	1479:1500	the formulation method	1479:1500	Thus, HAp/ChS microparticles and the formulation method of monoclonal antibodies using Zn could be useful in the treatment of local bone diseases.
33325979	9	59	theme	antibodies	1516:1525	arg1	useful					1545:1550	useful	1545:1550	useful	1545:1550	Thus, HAp/ChS microparticles and the formulation method of monoclonal antibodies using Zn could be useful in the treatment of local bone diseases.
33325979	9	59	theme	antibodies	1516:1525	arg1	microparticles					1460:1473	HAp/ChS microparticles	1452:1473	HAp/ChS microparticles	1452:1473	Thus, HAp/ChS microparticles and the formulation method of monoclonal antibodies using Zn could be useful in the treatment of local bone diseases.
33325979	5	60	theme	local	967:971	arg1	diseases					978:985	local bone diseases	967:985	local bone diseases	967:985	These results suggest a possible beneficial effect on the treatment for local bone diseases.
33325979	8	61	theme	formed	1260:1265	arg1	osteoid					1267:1273	the newly formed osteoid	1250:1273	the newly formed osteoid	1250:1273	After three weeks, the area of the newly formed osteoid around the drilled hole where HAp/ChS loaded with Sclmab and Zn was locally administered was significantly higher than that observed in the control group (normal saline).
33325979	1	62	theme	sulfate	271:277	arg1	method					336:341	their formulation method	318:341	their formulation method using zinc cations (Zn)	318:365	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	62	theme	sulfate	271:277	arg1	HAp/ChS					305:311	HAp/ChS	305:311	HAp/ChS	305:311	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	62	theme	sulfate	271:277	arg1	microparticles					289:302	hydroxyapatite/chondroitin sulfate composite microparticles	244:302	hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS)	244:312	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	62	theme	sulfate	271:277	arg1	carrier					180:186	a local delivery carrier	163:186	a local delivery carrier of bone metabolic proteins	163:213	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	62	theme	sulfate	271:277	arg1	properties					393:402	the in vitro release properties	372:402	the in vitro release properties of proteins from the microparticles	372:438	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	7	63	theme	young	1207:1211	arg1	rats					1213:1216	young rats	1207:1216	young rats	1207:1216	We prepared HAp/ChS microparticles loaded with Sclmab and locally administered the microparticles into a drilled hole in the distal femoral bone of young rats.
33325979	5	64	theme	bone	973:976	arg1	diseases					978:985	local bone diseases	967:985	local bone diseases	967:985	These results suggest a possible beneficial effect on the treatment for local bone diseases.
33325979	1	65	theme	local	165:169	arg1	method					336:341	their formulation method	318:341	their formulation method using zinc cations (Zn)	318:365	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	65	theme	local	165:169	arg1	microparticles					289:302	hydroxyapatite/chondroitin sulfate composite microparticles	244:302	hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS)	244:312	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	65	theme	local	165:169	arg1	carrier					180:186	a local delivery carrier	163:186	a local delivery carrier of bone metabolic proteins	163:213	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	65	theme	local	165:169	arg1	properties					393:402	the in vitro release properties	372:402	the in vitro release properties of proteins from the microparticles	372:438	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	7	66	theme	rats	1213:1216	arg1	bone					1199:1202	the distal femoral bone	1180:1202	the distal femoral bone of young rats	1180:1216	We prepared HAp/ChS microparticles loaded with Sclmab and locally administered the microparticles into a drilled hole in the distal femoral bone of young rats.
33325979	1	67	theme	composite	279:287	arg1	method					336:341	their formulation method	318:341	their formulation method using zinc cations (Zn)	318:365	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	67	theme	composite	279:287	arg1	HAp/ChS					305:311	HAp/ChS	305:311	HAp/ChS	305:311	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	67	theme	composite	279:287	arg1	microparticles					289:302	hydroxyapatite/chondroitin sulfate composite microparticles	244:302	hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS)	244:312	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	67	theme	composite	279:287	arg1	carrier					180:186	a local delivery carrier	163:186	a local delivery carrier of bone metabolic proteins	163:213	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	67	theme	composite	279:287	arg1	properties					393:402	the in vitro release properties	372:402	the in vitro release properties of proteins from the microparticles	372:438	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	0	68	theme	antibodies	60:69	arg1	administration					6:19	Local administration	0:19	Local administration	0:19	Local administration and enhanced release of bone metabolic antibodies from hydroxyapatite/chondroitin sulfate nanocomposite microparticles using zinc cations.
33325979	0	68	theme	antibodies	60:69	arg1	release					34:40	enhanced release	25:40	enhanced release of bone metabolic antibodies from hydroxyapatite/chondroitin sulfate	25:109	Local administration and enhanced release of bone metabolic antibodies from hydroxyapatite/chondroitin sulfate nanocomposite microparticles using zinc cations.
33325979	3	69	theme	HAp/ChS	663:669	arg1	microparticles					671:684	HAp/ChS microparticles	663:684	HAp/ChS microparticles	663:684	Adding Zn in the formulation of IgG loaded with HAp/ChS microparticles enhanced the release of antibodies from HAp/ChS in phosphate buffer saline.
33325979	9	70	theme	diseases	1583:1590	arg1	treatment					1559:1567	the treatment	1555:1567	the treatment of local bone diseases	1555:1590	Thus, HAp/ChS microparticles and the formulation method of monoclonal antibodies using Zn could be useful in the treatment of local bone diseases.
33325979	1	71	theme	delivery	171:178	arg1	method					336:341	their formulation method	318:341	their formulation method using zinc cations (Zn)	318:365	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	71	theme	delivery	171:178	arg1	microparticles					289:302	hydroxyapatite/chondroitin sulfate composite microparticles	244:302	hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS)	244:312	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	71	theme	delivery	171:178	arg1	carrier					180:186	a local delivery carrier	163:186	a local delivery carrier of bone metabolic proteins	163:213	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	1	71	theme	delivery	171:178	arg1	properties					393:402	the in vitro release properties	372:402	the in vitro release properties of proteins from the microparticles	372:438	As a local delivery carrier of bone metabolic proteins, we have previously reported hydroxyapatite/chondroitin sulfate composite microparticles (HAp/ChS) and their formulation method using zinc cations (Zn), and the in vitro release properties of proteins from the microparticles.
33325979	4	72	attach	released	807:814	arg2	Dmab					802:805	Dmab	802:805	Dmab released from HAp/ChS formulated with Zn	802:846	In addition, the biological activity of Dmab released from HAp/ChS formulated with Zn was significantly higher than that without Zn.
33325979	4	72	attach	released	807:814	arg1	HAp/ChS					821:827	HAp/ChS	821:827	HAp/ChS formulated with Zn	821:846	In addition, the biological activity of Dmab released from HAp/ChS formulated with Zn was significantly higher than that without Zn.
34900974	9	0	theme	multifunctional	1337:1351	arg1	enzyme					1353:1358	a multifunctional enzyme	1335:1358	a multifunctional enzyme	1335:1358	This suggested that the protein coded by A6180 might be a multifunctional enzyme.
34900974	9	0	theme	multifunctional	1337:1351	arg1	protein					1303:1309	the protein	1299:1309	the protein coded by A6180	1299:1324	This suggested that the protein coded by A6180 might be a multifunctional enzyme.
34900974	5	1	theme	heterologous	885:896	arg1	expression					898:907	heterologous expression	885:907	heterologous expression in E. coli	885:918	The recombinant overexpression vector pET22b-A6180 was constructed for heterologous expression in E. coli.
34900974	1	2	theme	medicinal	177:185	arg1	erinaceus					151:159	Hericium erinaceus	142:159	Hericium erinaceus	142:159	Hericium erinaceus is an important medicinal fungus in traditional Chinese medicine because of its polysaccharides and other natural products.
34900974	1	2	theme	medicinal	177:185	arg1	fungus					187:192	an important medicinal fungus	164:192	an important medicinal fungus in traditional Chinese medicine	164:224	Hericium erinaceus is an important medicinal fungus in traditional Chinese medicine because of its polysaccharides and other natural products.
34900974	6	3	theme	recombinant	953:963	arg1	protein					965:971	the recombinant protein	949:971	the recombinant protein	949:971	The enzymatic properties of the recombinant protein were investigated.
34900974	0	4	theme	erinaceus	88:96	arg1	Mutant					98:103	a Hericium erinaceus Mutant	77:103	a Hericium erinaceus Mutant with High Polysaccharide Production	77:139	Characterization and Heterologous Expression of UDP-Glucose 4-Epimerase From a Hericium erinaceus Mutant with High Polysaccharide Production.
34900974	0	5	with	Mutant	98:103	arg1	Production					130:139	High Polysaccharide Production	110:139	High Polysaccharide Production	110:139	Characterization and Heterologous Expression of UDP-Glucose 4-Epimerase From a Hericium erinaceus Mutant with High Polysaccharide Production.
34900974	6	6	theme	enzymatic	925:933	arg1	properties					935:944	The enzymatic properties	921:944	The enzymatic properties of the recombinant protein	921:971	The enzymatic properties of the recombinant protein were investigated.
34900974	0	7	theme	High	110:113	arg1	Production					130:139	High Polysaccharide Production	110:139	High Polysaccharide Production	110:139	Characterization and Heterologous Expression of UDP-Glucose 4-Epimerase From a Hericium erinaceus Mutant with High Polysaccharide Production.
34900974	0	8	from	Characterization	0:15	arg1	Mutant					98:103	a Hericium erinaceus Mutant	77:103	a Hericium erinaceus Mutant with High Polysaccharide Production	77:139	Characterization and Heterologous Expression of UDP-Glucose 4-Epimerase From a Hericium erinaceus Mutant with High Polysaccharide Production.
34900974	1	9	from	fungus	187:192	arg1	medicine					217:224	traditional Chinese medicine	197:224	traditional Chinese medicine	197:224	Hericium erinaceus is an important medicinal fungus in traditional Chinese medicine because of its polysaccharides and other natural products.
34900974	3	10	theme	high	560:563	arg1	production					565:574	high production	560:574	high production of active polysaccharides	560:600	In previous studies, A6180, encoding a putative UDP-glucose 4-epimerase (UGE) in an H. erinaceus mutant with high production of active polysaccharides, was significantly upregulated.
34900974	4	11	theme	H.	697:698	arg1	erinaceus					700:708	H. erinaceus	697:708	H. erinaceus	697:708	Since there is no reliable genetic manipulation technology for H. erinaceus, we employed Escherichia coli and Saccharomyces cerevisiae to study the function and activity of A6180.
34900974	8	12	located	detected	1209:1216	arg1	composition					1240:1250	the polysaccharide composition	1221:1250	the polysaccharide composition of the yeast transformant	1221:1276	In addition, when A6180 was introduced into S. cerevisiae BY4742, xylose was detected in the polysaccharide composition of the yeast transformant.
34900974	8	12	located	detected	1209:1216	arg2	xylose					1198:1203	xylose	1198:1203	xylose	1198:1203	In addition, when A6180 was introduced into S. cerevisiae BY4742, xylose was detected in the polysaccharide composition of the yeast transformant.
34900974	8	12	located	detected	1209:1216	arg1	addition					1135:1142	addition	1135:1142	addition	1135:1142	In addition, when A6180 was introduced into S. cerevisiae BY4742, xylose was detected in the polysaccharide composition of the yeast transformant.
34900974	6	13	theme	protein	965:971	arg1	properties					935:944	The enzymatic properties	921:944	The enzymatic properties of the recombinant protein	921:971	The enzymatic properties of the recombinant protein were investigated.
34900974	7	14	theme	recombinant	1011:1021	arg1	A6180					1023:1027	the recombinant A6180	1007:1027	the recombinant A6180	1007:1027	It showed that the recombinant A6180 could strongly convert UDP-α-D-glucose into UDP-α-D-galactose under optimal conditions (pH 6.0, 30°C).
34900974	8	15	theme	polysaccharide	1225:1238	arg1	composition					1240:1250	the polysaccharide composition	1221:1250	the polysaccharide composition of the yeast transformant	1221:1276	In addition, when A6180 was introduced into S. cerevisiae BY4742, xylose was detected in the polysaccharide composition of the yeast transformant.
34900974	4	16	theme	reliable	652:659	arg1	manipulation					669:680	no reliable genetic manipulation	649:680	no reliable genetic manipulation technology for H. erinaceus	649:708	Since there is no reliable genetic manipulation technology for H. erinaceus, we employed Escherichia coli and Saccharomyces cerevisiae to study the function and activity of A6180.
34900974	11	17	theme	important	1513:1521	arg1	role					1523:1526	an important role	1510:1526	an important role	1510:1526	These results indicate that A6180 plays an important role in the structure and activity of polysaccharides.
34900974	2	18	theme	central	431:437	arg1	metabolism					439:448	central metabolism	431:448	central metabolism	431:448	Compared terpenoids and polyketides, the analysis of synthetic pathway of polysaccharides is more difficult because of the many genes involved in central metabolism.
34900974	0	19	theme	Polysaccharide	115:128	arg1	Production					130:139	High Polysaccharide Production	110:139	High Polysaccharide Production	110:139	Characterization and Heterologous Expression of UDP-Glucose 4-Epimerase From a Hericium erinaceus Mutant with High Polysaccharide Production.
34900974	3	20	with	mutant	548:553	arg1	production					565:574	high production	560:574	high production of active polysaccharides	560:600	In previous studies, A6180, encoding a putative UDP-glucose 4-epimerase (UGE) in an H. erinaceus mutant with high production of active polysaccharides, was significantly upregulated.
34900974	4	21	theme	A6180	807:811	arg1	function					782:789	function	782:789	function	782:789	Since there is no reliable genetic manipulation technology for H. erinaceus, we employed Escherichia coli and Saccharomyces cerevisiae to study the function and activity of A6180.
34900974	4	21	theme	A6180	807:811	arg1	activity					795:802	activity	795:802	activity	795:802	Since there is no reliable genetic manipulation technology for H. erinaceus, we employed Escherichia coli and Saccharomyces cerevisiae to study the function and activity of A6180.
34900974	7	22	dep	conditions	1105:1114	arg1	pH					1117:1118	pH 6.0	1117:1122	pH 6.0	1117:1122	It showed that the recombinant A6180 could strongly convert UDP-α-D-glucose into UDP-α-D-galactose under optimal conditions (pH 6.0, 30°C).
34900974	7	22	dep	conditions	1105:1114	arg1	30°C					1125:1128	30°C	1125:1128	30°C	1125:1128	It showed that the recombinant A6180 could strongly convert UDP-α-D-glucose into UDP-α-D-galactose under optimal conditions (pH 6.0, 30°C).
34900974	1	23	theme	traditional	197:207	arg1	medicine					217:224	traditional Chinese medicine	197:224	traditional Chinese medicine	197:224	Hericium erinaceus is an important medicinal fungus in traditional Chinese medicine because of its polysaccharides and other natural products.
34900974	3	24	theme	erinaceus	538:546	arg1	mutant					548:553	an H. erinaceus mutant	532:553	an H. erinaceus mutant with high production of active polysaccharides	532:600	In previous studies, A6180, encoding a putative UDP-glucose 4-epimerase (UGE) in an H. erinaceus mutant with high production of active polysaccharides, was significantly upregulated.
34900974	3	25	theme	polysaccharides	586:600	arg1	production					565:574	high production	560:574	high production of active polysaccharides	560:600	In previous studies, A6180, encoding a putative UDP-glucose 4-epimerase (UGE) in an H. erinaceus mutant with high production of active polysaccharides, was significantly upregulated.
34900974	3	26	theme	H.	535:536	arg1	mutant					548:553	an H. erinaceus mutant	532:553	an H. erinaceus mutant with high production of active polysaccharides	532:600	In previous studies, A6180, encoding a putative UDP-glucose 4-epimerase (UGE) in an H. erinaceus mutant with high production of active polysaccharides, was significantly upregulated.
34900974	1	27	theme	Chinese	209:215	arg1	medicine					217:224	traditional Chinese medicine	197:224	traditional Chinese medicine	197:224	Hericium erinaceus is an important medicinal fungus in traditional Chinese medicine because of its polysaccharides and other natural products.
34900974	8	28	dep	S.	1176:1177	arg1	cerevisiae					1179:1188	cerevisiae	1179:1188	cerevisiae	1179:1188	In addition, when A6180 was introduced into S. cerevisiae BY4742, xylose was detected in the polysaccharide composition of the yeast transformant.
34900974	10	29	with	polysaccharides	1375:1389	arg1	composition					1402:1412	a new composition	1396:1412	a new composition of sugars	1396:1422	The generated polysaccharides with a new composition of sugars showed enhanced macrophage activity in vitro.
34900974	10	30	theme	sugars	1417:1422	arg1	composition					1402:1412	a new composition	1396:1412	a new composition of sugars	1396:1422	The generated polysaccharides with a new composition of sugars showed enhanced macrophage activity in vitro.
34900974	2	31	theme	pathway	348:354	arg1	analysis					326:333	the analysis	322:333	the analysis of synthetic pathway of polysaccharides	322:373	Compared terpenoids and polyketides, the analysis of synthetic pathway of polysaccharides is more difficult because of the many genes involved in central metabolism.
34900974	2	31	theme	pathway	348:354	arg1	difficult					383:391	difficult	383:391	difficult	383:391	Compared terpenoids and polyketides, the analysis of synthetic pathway of polysaccharides is more difficult because of the many genes involved in central metabolism.
34900974	2	32	theme	genes	413:417	arg1	analysis					326:333	the analysis	322:333	the analysis of synthetic pathway of polysaccharides	322:373	Compared terpenoids and polyketides, the analysis of synthetic pathway of polysaccharides is more difficult because of the many genes involved in central metabolism.
34900974	2	32	theme	genes	413:417	arg1	difficult					383:391	difficult	383:391	difficult	383:391	Compared terpenoids and polyketides, the analysis of synthetic pathway of polysaccharides is more difficult because of the many genes involved in central metabolism.
34900974	8	33	theme	yeast	1259:1263	arg1	transformant					1265:1276	the yeast transformant	1255:1276	the yeast transformant	1255:1276	In addition, when A6180 was introduced into S. cerevisiae BY4742, xylose was detected in the polysaccharide composition of the yeast transformant.
34900974	12	34	theme	polysaccharide-producing	1682:1705	arg1	mushrooms					1707:1715	polysaccharide-producing mushrooms	1682:1715	polysaccharide-producing mushrooms	1682:1715	It is a promising strategy for producing polysaccharides with higher activity by introducing A6180 into polysaccharide-producing mushrooms.
34900974	2	35	theme	synthetic	338:346	arg1	pathway					348:354	synthetic pathway	338:354	synthetic pathway of polysaccharides	338:373	Compared terpenoids and polyketides, the analysis of synthetic pathway of polysaccharides is more difficult because of the many genes involved in central metabolism.
34900974	3	36	theme	putative	490:497	arg1	UGE					524:526	UGE	524:526	UGE	524:526	In previous studies, A6180, encoding a putative UDP-glucose 4-epimerase (UGE) in an H. erinaceus mutant with high production of active polysaccharides, was significantly upregulated.
34900974	3	36	theme	putative	490:497	arg1	4-epimerase					511:521	a putative UDP-glucose 4-epimerase	488:521	a putative UDP-glucose 4-epimerase (UGE)	488:527	In previous studies, A6180, encoding a putative UDP-glucose 4-epimerase (UGE) in an H. erinaceus mutant with high production of active polysaccharides, was significantly upregulated.
34900974	0	37	theme	Heterologous	21:32	arg1	Expression					34:43	Heterologous Expression	21:43	Heterologous Expression	21:43	Characterization and Heterologous Expression of UDP-Glucose 4-Epimerase From a Hericium erinaceus Mutant with High Polysaccharide Production.
34900974	12	38	theme	promising	1586:1594	arg1	It					1578:1579	It	1578:1579	It	1578:1579	It is a promising strategy for producing polysaccharides with higher activity by introducing A6180 into polysaccharide-producing mushrooms.
34900974	12	38	theme	promising	1586:1594	arg1	strategy					1596:1603	a promising strategy	1584:1603	a promising strategy for producing polysaccharides with higher activity by introducing A6180 into polysaccharide-producing mushrooms	1584:1715	It is a promising strategy for producing polysaccharides with higher activity by introducing A6180 into polysaccharide-producing mushrooms.
34900974	0	39	from	Mutant	98:103	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and Heterologous Expression of UDP-Glucose 4-Epimerase From a Hericium erinaceus Mutant with High Polysaccharide Production.
34900974	0	39	from	Mutant	98:103	arg1	Expression					34:43	Heterologous Expression	21:43	Heterologous Expression	21:43	Characterization and Heterologous Expression of UDP-Glucose 4-Epimerase From a Hericium erinaceus Mutant with High Polysaccharide Production.
34900974	0	39	from	Mutant	98:103	arg1	4-Epimerase					60:70	UDP-Glucose 4-Epimerase	48:70	UDP-Glucose 4-Epimerase From a Hericium erinaceus Mutant with High Polysaccharide Production	48:139	Characterization and Heterologous Expression of UDP-Glucose 4-Epimerase From a Hericium erinaceus Mutant with High Polysaccharide Production.
34900974	3	40	theme	active	579:584	arg1	polysaccharides					586:600	active polysaccharides	579:600	active polysaccharides	579:600	In previous studies, A6180, encoding a putative UDP-glucose 4-epimerase (UGE) in an H. erinaceus mutant with high production of active polysaccharides, was significantly upregulated.
34900974	10	41	theme	generated	1365:1373	arg1	polysaccharides					1375:1389	The generated polysaccharides	1361:1389	The generated polysaccharides with a new composition of sugars	1361:1422	The generated polysaccharides with a new composition of sugars showed enhanced macrophage activity in vitro.
34900974	3	42	theme	UDP-glucose	499:509	arg1	UGE					524:526	UGE	524:526	UGE	524:526	In previous studies, A6180, encoding a putative UDP-glucose 4-epimerase (UGE) in an H. erinaceus mutant with high production of active polysaccharides, was significantly upregulated.
34900974	3	42	theme	UDP-glucose	499:509	arg1	4-epimerase					511:521	a putative UDP-glucose 4-epimerase	488:521	a putative UDP-glucose 4-epimerase (UGE)	488:527	In previous studies, A6180, encoding a putative UDP-glucose 4-epimerase (UGE) in an H. erinaceus mutant with high production of active polysaccharides, was significantly upregulated.
34900974	0	43	theme	UDP-Glucose	48:58	arg1	4-Epimerase					60:70	UDP-Glucose 4-Epimerase	48:70	UDP-Glucose 4-Epimerase From a Hericium erinaceus Mutant with High Polysaccharide Production	48:139	Characterization and Heterologous Expression of UDP-Glucose 4-Epimerase From a Hericium erinaceus Mutant with High Polysaccharide Production.
34900974	4	44	dep	function	782:789	arg1	the					778:780	the	778:780	the	778:780	Since there is no reliable genetic manipulation technology for H. erinaceus, we employed Escherichia coli and Saccharomyces cerevisiae to study the function and activity of A6180.
34900974	3	45	theme	previous	454:461	arg1	studies					463:469	previous studies	454:469	previous studies	454:469	In previous studies, A6180, encoding a putative UDP-glucose 4-epimerase (UGE) in an H. erinaceus mutant with high production of active polysaccharides, was significantly upregulated.
34900974	5	46	theme	recombinant	818:828	arg1	vector					845:850	The recombinant overexpression vector pET22b-A6180	814:863	The recombinant overexpression vector pET22b-A6180	814:863	The recombinant overexpression vector pET22b-A6180 was constructed for heterologous expression in E. coli.
34900974	4	47	theme	manipulation	669:680	arg1	technology					682:691	no reliable genetic manipulation technology	649:691	no reliable genetic manipulation technology for H. erinaceus	649:708	Since there is no reliable genetic manipulation technology for H. erinaceus, we employed Escherichia coli and Saccharomyces cerevisiae to study the function and activity of A6180.
34900974	5	48	theme	overexpression	830:843	arg1	vector					845:850	The recombinant overexpression vector pET22b-A6180	814:863	The recombinant overexpression vector pET22b-A6180	814:863	The recombinant overexpression vector pET22b-A6180 was constructed for heterologous expression in E. coli.
34900974	8	49	theme	transformant	1265:1276	arg1	composition					1240:1250	the polysaccharide composition	1221:1250	the polysaccharide composition of the yeast transformant	1221:1276	In addition, when A6180 was introduced into S. cerevisiae BY4742, xylose was detected in the polysaccharide composition of the yeast transformant.
34900974	0	50	theme	4-Epimerase	60:70	arg1	Characterization					0:15	Characterization	0:15	Characterization	0:15	Characterization and Heterologous Expression of UDP-Glucose 4-Epimerase From a Hericium erinaceus Mutant with High Polysaccharide Production.
34900974	0	50	theme	4-Epimerase	60:70	arg1	Expression					34:43	Heterologous Expression	21:43	Heterologous Expression	21:43	Characterization and Heterologous Expression of UDP-Glucose 4-Epimerase From a Hericium erinaceus Mutant with High Polysaccharide Production.
34900974	10	51	theme	new	1398:1400	arg1	composition					1402:1412	a new composition	1396:1412	a new composition of sugars	1396:1422	The generated polysaccharides with a new composition of sugars showed enhanced macrophage activity in vitro.
34900974	1	52	theme	Hericium	142:149	arg1	erinaceus					151:159	Hericium erinaceus	142:159	Hericium erinaceus	142:159	Hericium erinaceus is an important medicinal fungus in traditional Chinese medicine because of its polysaccharides and other natural products.
34900974	1	52	theme	Hericium	142:149	arg1	fungus					187:192	an important medicinal fungus	164:192	an important medicinal fungus in traditional Chinese medicine	164:224	Hericium erinaceus is an important medicinal fungus in traditional Chinese medicine because of its polysaccharides and other natural products.
34900974	12	53	with	polysaccharides	1619:1633	arg1	activity					1647:1654	higher activity	1640:1654	higher activity	1640:1654	It is a promising strategy for producing polysaccharides with higher activity by introducing A6180 into polysaccharide-producing mushrooms.
34900974	5	54	from	expression	898:907	arg1	coli					915:918	E. coli	912:918	E. coli	912:918	The recombinant overexpression vector pET22b-A6180 was constructed for heterologous expression in E. coli.
34900974	11	55	theme	polysaccharides	1561:1575	arg1	activity					1549:1556	activity	1549:1556	activity	1549:1556	These results indicate that A6180 plays an important role in the structure and activity of polysaccharides.
34900974	11	55	theme	polysaccharides	1561:1575	arg1	structure					1535:1543	structure	1535:1543	structure	1535:1543	These results indicate that A6180 plays an important role in the structure and activity of polysaccharides.
34900974	7	56	theme	optimal	1097:1103	arg1	conditions					1105:1114	optimal conditions	1097:1114	optimal conditions (pH 6.0, 30°C)	1097:1129	It showed that the recombinant A6180 could strongly convert UDP-α-D-glucose into UDP-α-D-galactose under optimal conditions (pH 6.0, 30°C).
34900974	11	57	dep	structure	1535:1543	arg1	the					1531:1533	the	1531:1533	the	1531:1533	These results indicate that A6180 plays an important role in the structure and activity of polysaccharides.
34900974	10	58	theme	macrophage	1440:1449	arg1	activity					1451:1458	enhanced macrophage activity	1431:1458	enhanced macrophage activity	1431:1458	The generated polysaccharides with a new composition of sugars showed enhanced macrophage activity in vitro.
34900974	2	59	theme	polysaccharides	359:373	arg1	pathway					348:354	synthetic pathway	338:354	synthetic pathway of polysaccharides	338:373	Compared terpenoids and polyketides, the analysis of synthetic pathway of polysaccharides is more difficult because of the many genes involved in central metabolism.
34900974	1	60	theme	other	261:265	arg1	products					275:282	other natural products	261:282	other natural products	261:282	Hericium erinaceus is an important medicinal fungus in traditional Chinese medicine because of its polysaccharides and other natural products.
34900974	0	61	theme	Hericium	79:86	arg1	Mutant					98:103	a Hericium erinaceus Mutant	77:103	a Hericium erinaceus Mutant with High Polysaccharide Production	77:139	Characterization and Heterologous Expression of UDP-Glucose 4-Epimerase From a Hericium erinaceus Mutant with High Polysaccharide Production.
34900974	10	62	theme	enhanced	1431:1438	arg1	activity					1451:1458	enhanced macrophage activity	1431:1458	enhanced macrophage activity	1431:1458	The generated polysaccharides with a new composition of sugars showed enhanced macrophage activity in vitro.
34900974	4	63	theme	genetic	661:667	arg1	manipulation					669:680	no reliable genetic manipulation	649:680	no reliable genetic manipulation technology for H. erinaceus	649:708	Since there is no reliable genetic manipulation technology for H. erinaceus, we employed Escherichia coli and Saccharomyces cerevisiae to study the function and activity of A6180.
34900974	2	64	theme	many	408:411	arg1	genes					413:417	the many genes	404:417	the many genes involved in central metabolism	404:448	Compared terpenoids and polyketides, the analysis of synthetic pathway of polysaccharides is more difficult because of the many genes involved in central metabolism.
34900974	1	65	theme	natural	267:273	arg1	products					275:282	other natural products	261:282	other natural products	261:282	Hericium erinaceus is an important medicinal fungus in traditional Chinese medicine because of its polysaccharides and other natural products.
34900974	0	66	from	Expression	34:43	arg1	Mutant					98:103	a Hericium erinaceus Mutant	77:103	a Hericium erinaceus Mutant with High Polysaccharide Production	77:139	Characterization and Heterologous Expression of UDP-Glucose 4-Epimerase From a Hericium erinaceus Mutant with High Polysaccharide Production.
34900974	1	67	theme	important	167:175	arg1	erinaceus					151:159	Hericium erinaceus	142:159	Hericium erinaceus	142:159	Hericium erinaceus is an important medicinal fungus in traditional Chinese medicine because of its polysaccharides and other natural products.
34900974	1	67	theme	important	167:175	arg1	fungus					187:192	an important medicinal fungus	164:192	an important medicinal fungus in traditional Chinese medicine	164:224	Hericium erinaceus is an important medicinal fungus in traditional Chinese medicine because of its polysaccharides and other natural products.
34900974	8	68	theme	S.	1176:1177	arg1	BY4742					1190:1195	S. cerevisiae BY4742	1176:1195	S. cerevisiae BY4742	1176:1195	In addition, when A6180 was introduced into S. cerevisiae BY4742, xylose was detected in the polysaccharide composition of the yeast transformant.
34900974	12	69	theme	higher	1640:1645	arg1	activity					1647:1654	higher activity	1640:1654	higher activity	1640:1654	It is a promising strategy for producing polysaccharides with higher activity by introducing A6180 into polysaccharide-producing mushrooms.
32019174	0	0	theme	Microbiota	69:78	arg1	Composition					80:90	Gut Microbiota Composition	65:90	Gut Microbiota Composition	65:90	In Vitro Evaluation of Different Prebiotics on the Modulation of Gut Microbiota Composition and Function in Morbid Obese and Normal-Weight Subjects.
32019174	0	1	from	Evaluation	9:18	arg1	Modulation					51:60	the Modulation	47:60	the Modulation of Gut Microbiota Composition and Function in Morbid Obese and Normal-Weight Subjects	47:146	In Vitro Evaluation of Different Prebiotics on the Modulation of Gut Microbiota Composition and Function in Morbid Obese and Normal-Weight Subjects.
32019174	9	2	from	substantial	1398:1408	arg1	Faecalibacterium					1439:1454	Faecalibacterium	1439:1454	Faecalibacterium	1439:1454	The modulation with prebiotics led to significant increases in the Bacteroides group and Faecalibacterium in obese subjects, whereas in normal-weight individuals, substantial rises in Bifidobacterium and Faecalibacterium were appreciated.
32019174	9	2	from	substantial	1398:1408	arg1	Bifidobacterium					1419:1433	Bifidobacterium	1419:1433	Bifidobacterium	1419:1433	The modulation with prebiotics led to significant increases in the Bacteroides group and Faecalibacterium in obese subjects, whereas in normal-weight individuals, substantial rises in Bifidobacterium and Faecalibacterium were appreciated.
32019174	10	3	theme	tested	1550:1555	arg1	compounds					1557:1565	the tested compounds	1546:1565	the tested compounds	1546:1565	Notably, the results obtained showed differences in the responses among the tested compounds but also among the studied human populations, indicating the need for developing population-specific products.
32019174	1	4	theme	environmental	243:255	arg1	factors					257:263	certain intrinsic and environmental factors	221:263	certain intrinsic and environmental factors	221:263	The gut microbiota remains relatively stable during adulthood; however, certain intrinsic and environmental factors can lead to microbiota dysbiosis.
32019174	0	5	theme	Gut	65:67	arg1	Composition					80:90	Gut Microbiota Composition	65:90	Gut Microbiota Composition	65:90	In Vitro Evaluation of Different Prebiotics on the Modulation of Gut Microbiota Composition and Function in Morbid Obese and Normal-Weight Subjects.
32019174	8	6	from	compound	1197:1204	arg1	general					1170:1176	general	1170:1176	general	1170:1176	In general, 1-kestose was the compound showing the largest effects.
32019174	7	7	theme	intestinal	1145:1154	arg1	line					1161:1164	an intestinal cell line	1142:1164	an intestinal cell line	1142:1164	Moreover, the functionality of the microbiota before and after prebiotic-modulation was determined in an in vitro model of interaction with an intestinal cell line.
32019174	5	8	theme	microbiota	812:821	arg1	composition					823:833	the microbiota composition	808:833	the microbiota composition (qPCR and 16S rRNA gene profiling)	808:868	Their impact on the gut microbiota was assessed by monitoring gas production and evaluating changes in the microbiota composition (qPCR and 16S rRNA gene profiling) and metabolic activity (gas chromatography).
32019174	1	9	theme	gut	153:155	arg1	microbiota					157:166	The gut microbiota	149:166	The gut microbiota	149:166	The gut microbiota remains relatively stable during adulthood; however, certain intrinsic and environmental factors can lead to microbiota dysbiosis.
32019174	5	10	dep	qPCR	836:839	arg1	profiling					859:867	gene profiling	854:867	gene profiling	854:867	Their impact on the gut microbiota was assessed by monitoring gas production and evaluating changes in the microbiota composition (qPCR and 16S rRNA gene profiling) and metabolic activity (gas chromatography).
32019174	0	11	theme	Composition	80:90	arg1	Modulation					51:60	the Modulation	47:60	the Modulation of Gut Microbiota Composition and Function in Morbid Obese and Normal-Weight Subjects	47:146	In Vitro Evaluation of Different Prebiotics on the Modulation of Gut Microbiota Composition and Function in Morbid Obese and Normal-Weight Subjects.
32019174	9	12	dep	substantial	1398:1408	arg1	rises					1410:1414	rises	1410:1414	rises	1410:1414	The modulation with prebiotics led to significant increases in the Bacteroides group and Faecalibacterium in obese subjects, whereas in normal-weight individuals, substantial rises in Bifidobacterium and Faecalibacterium were appreciated.
32019174	6	13	from	effect	933:938	arg1	species					963:969	the bifidobacterial species	943:969	the bifidobacterial species	943:969	Additionally, the effect on the bifidobacterial species was assessed (ITS-sequencing).
32019174	9	14	with	modulation	1239:1248	arg1	prebiotics					1255:1264	prebiotics	1255:1264	prebiotics	1255:1264	The modulation with prebiotics led to significant increases in the Bacteroides group and Faecalibacterium in obese subjects, whereas in normal-weight individuals, substantial rises in Bifidobacterium and Faecalibacterium were appreciated.
32019174	5	15	theme	metabolic	874:882	arg1	activity					884:891	metabolic activity	874:891	metabolic activity (gas chromatography)	874:912	Their impact on the gut microbiota was assessed by monitoring gas production and evaluating changes in the microbiota composition (qPCR and 16S rRNA gene profiling) and metabolic activity (gas chromatography).
32019174	5	15	theme	metabolic	874:882	arg1	chromatography					898:911	gas chromatography	894:911	gas chromatography	894:911	Their impact on the gut microbiota was assessed by monitoring gas production and evaluating changes in the microbiota composition (qPCR and 16S rRNA gene profiling) and metabolic activity (gas chromatography).
32019174	5	16	theme	gas	767:769	arg1	production					771:780	gas production	767:780	gas production	767:780	Their impact on the gut microbiota was assessed by monitoring gas production and evaluating changes in the microbiota composition (qPCR and 16S rRNA gene profiling) and metabolic activity (gas chromatography).
32019174	5	17	dep	composition	823:833	arg1	qPCR					836:839	qPCR	836:839	qPCR	836:839	Their impact on the gut microbiota was assessed by monitoring gas production and evaluating changes in the microbiota composition (qPCR and 16S rRNA gene profiling) and metabolic activity (gas chromatography).
32019174	5	17	dep	composition	823:833	arg1	rRNA					849:852	16S rRNA	845:852	16S rRNA	845:852	Their impact on the gut microbiota was assessed by monitoring gas production and evaluating changes in the microbiota composition (qPCR and 16S rRNA gene profiling) and metabolic activity (gas chromatography).
32019174	10	18	theme	population-specific	1648:1666	arg1	products					1668:1675	population-specific products	1648:1675	population-specific products	1648:1675	Notably, the results obtained showed differences in the responses among the tested compounds but also among the studied human populations, indicating the need for developing population-specific products.
32019174	2	19	theme	models	414:419	arg1	development					390:400	previous development	381:400	previous development of in vitro models	381:419	Its restoration towards a healthy condition using best-suited prebiotics requires previous development of in vitro models for evaluating their functionality.
32019174	0	20	theme	Function	96:103	arg1	Modulation					51:60	the Modulation	47:60	the Modulation of Gut Microbiota Composition and Function in Morbid Obese and Normal-Weight Subjects	47:146	In Vitro Evaluation of Different Prebiotics on the Modulation of Gut Microbiota Composition and Function in Morbid Obese and Normal-Weight Subjects.
32019174	9	21	theme	obese	1344:1348	arg1	subjects					1350:1357	obese subjects	1344:1357	obese subjects	1344:1357	The modulation with prebiotics led to significant increases in the Bacteroides group and Faecalibacterium in obese subjects, whereas in normal-weight individuals, substantial rises in Bifidobacterium and Faecalibacterium were appreciated.
32019174	7	22	dep	in	1107:1108	arg1	vitro					1110:1114	vitro	1110:1114	vitro	1110:1114	Moreover, the functionality of the microbiota before and after prebiotic-modulation was determined in an in vitro model of interaction with an intestinal cell line.
32019174	3	23	dep	healthy	516:522	arg1	normal-weight					524:536	normal-weight	524:536	normal-weight	524:536	Herein, we carried out fecal cultures with microbiota from healthy normal-weight and morbid obese adults.
32019174	9	24	dep	led	1266:1268	arg1	whereas					1360:1366	whereas	1360:1366	whereas	1360:1366	The modulation with prebiotics led to significant increases in the Bacteroides group and Faecalibacterium in obese subjects, whereas in normal-weight individuals, substantial rises in Bifidobacterium and Faecalibacterium were appreciated.
32019174	3	25	theme	obese	549:553	arg1	adults					555:560	healthy normal-weight and morbid obese adults	516:560	healthy normal-weight and morbid obese adults	516:560	Herein, we carried out fecal cultures with microbiota from healthy normal-weight and morbid obese adults.
32019174	7	26	theme	in	1107:1108	arg1	model					1116:1120	an in vitro model	1104:1120	an in vitro model of interaction with an intestinal cell line	1104:1164	Moreover, the functionality of the microbiota before and after prebiotic-modulation was determined in an in vitro model of interaction with an intestinal cell line.
32019174	9	27	from	Faecalibacterium	1439:1454	arg1	substantial					1398:1408	substantial	1398:1408	substantial	1398:1408	The modulation with prebiotics led to significant increases in the Bacteroides group and Faecalibacterium in obese subjects, whereas in normal-weight individuals, substantial rises in Bifidobacterium and Faecalibacterium were appreciated.
32019174	2	28	theme	in	405:406	arg1	models					414:419	in vitro models	405:419	in vitro models	405:419	Its restoration towards a healthy condition using best-suited prebiotics requires previous development of in vitro models for evaluating their functionality.
32019174	0	29	theme	In	0:1	arg1	Evaluation					9:18	In Vitro Evaluation	0:18	In Vitro Evaluation of Different Prebiotics on the Modulation of Gut Microbiota Composition and Function in Morbid Obese and Normal-Weight Subjects.	0:147	In Vitro Evaluation of Different Prebiotics on the Modulation of Gut Microbiota Composition and Function in Morbid Obese and Normal-Weight Subjects.
32019174	10	30	theme	studied	1586:1592	arg1	populations					1600:1610	the studied human populations	1582:1610	the studied human populations	1582:1610	Notably, the results obtained showed differences in the responses among the tested compounds but also among the studied human populations, indicating the need for developing population-specific products.
32019174	5	31	theme	gas	894:896	arg1	activity					884:891	metabolic activity	874:891	metabolic activity (gas chromatography)	874:912	Their impact on the gut microbiota was assessed by monitoring gas production and evaluating changes in the microbiota composition (qPCR and 16S rRNA gene profiling) and metabolic activity (gas chromatography).
32019174	5	31	theme	gas	894:896	arg1	chromatography					898:911	gas chromatography	894:911	gas chromatography	894:911	Their impact on the gut microbiota was assessed by monitoring gas production and evaluating changes in the microbiota composition (qPCR and 16S rRNA gene profiling) and metabolic activity (gas chromatography).
32019174	5	32	theme	16S	845:847	arg1	rRNA					849:852	16S rRNA	845:852	16S rRNA	845:852	Their impact on the gut microbiota was assessed by monitoring gas production and evaluating changes in the microbiota composition (qPCR and 16S rRNA gene profiling) and metabolic activity (gas chromatography).
32019174	1	33	theme	microbiota	277:286	arg1	dysbiosis					288:296	microbiota dysbiosis	277:296	microbiota dysbiosis	277:296	The gut microbiota remains relatively stable during adulthood; however, certain intrinsic and environmental factors can lead to microbiota dysbiosis.
32019174	0	34	theme	Obese	115:119	arg1	Subjects					139:146	Morbid Obese and Normal-Weight Subjects	108:146	Morbid Obese and Normal-Weight Subjects	108:146	In Vitro Evaluation of Different Prebiotics on the Modulation of Gut Microbiota Composition and Function in Morbid Obese and Normal-Weight Subjects.
32019174	3	35	theme	fecal	480:484	arg1	cultures					486:493	fecal cultures	480:493	fecal cultures with microbiota from healthy normal-weight and morbid obese adults	480:560	Herein, we carried out fecal cultures with microbiota from healthy normal-weight and morbid obese adults.
32019174	4	36	theme	inulin-type	605:615	arg1	1-kestose					627:635	1-kestose	627:635	1-kestose	627:635	Cultures were supplemented with different inulin-type fructans (1-kestose, Actilight, P95, Synergy1 and Inulin) and a galactooligosaccharide.
32019174	4	36	theme	inulin-type	605:615	arg1	Synergy1					654:661	Synergy1	654:661	Synergy1	654:661	Cultures were supplemented with different inulin-type fructans (1-kestose, Actilight, P95, Synergy1 and Inulin) and a galactooligosaccharide.
32019174	4	36	theme	inulin-type	605:615	arg1	Inulin					667:672	Inulin	667:672	Inulin	667:672	Cultures were supplemented with different inulin-type fructans (1-kestose, Actilight, P95, Synergy1 and Inulin) and a galactooligosaccharide.
32019174	4	36	theme	inulin-type	605:615	arg1	Actilight					638:646	Actilight	638:646	Actilight	638:646	Cultures were supplemented with different inulin-type fructans (1-kestose, Actilight, P95, Synergy1 and Inulin) and a galactooligosaccharide.
32019174	4	36	theme	inulin-type	605:615	arg1	P95					649:651	P95	649:651	P95	649:651	Cultures were supplemented with different inulin-type fructans (1-kestose, Actilight, P95, Synergy1 and Inulin) and a galactooligosaccharide.
32019174	4	36	theme	inulin-type	605:615	arg1	fructans					617:624	different inulin-type fructans	595:624	different inulin-type fructans (1-kestose, Actilight, P95, Synergy1 and Inulin)	595:673	Cultures were supplemented with different inulin-type fructans (1-kestose, Actilight, P95, Synergy1 and Inulin) and a galactooligosaccharide.
32019174	0	37	theme	Morbid	108:113	arg1	Subjects					139:146	Morbid Obese and Normal-Weight Subjects	108:146	Morbid Obese and Normal-Weight Subjects	108:146	In Vitro Evaluation of Different Prebiotics on the Modulation of Gut Microbiota Composition and Function in Morbid Obese and Normal-Weight Subjects.
32019174	9	38	from	group	1314:1318	arg1	subjects					1350:1357	obese subjects	1344:1357	obese subjects	1344:1357	The modulation with prebiotics led to significant increases in the Bacteroides group and Faecalibacterium in obese subjects, whereas in normal-weight individuals, substantial rises in Bifidobacterium and Faecalibacterium were appreciated.
32019174	7	39	with	interaction	1125:1135	arg1	line					1161:1164	an intestinal cell line	1142:1164	an intestinal cell line	1142:1164	Moreover, the functionality of the microbiota before and after prebiotic-modulation was determined in an in vitro model of interaction with an intestinal cell line.
32019174	0	40	theme	Prebiotics	33:42	arg1	Evaluation					9:18	In Vitro Evaluation	0:18	In Vitro Evaluation of Different Prebiotics on the Modulation of Gut Microbiota Composition and Function in Morbid Obese and Normal-Weight Subjects.	0:147	In Vitro Evaluation of Different Prebiotics on the Modulation of Gut Microbiota Composition and Function in Morbid Obese and Normal-Weight Subjects.
32019174	4	41	theme	different	595:603	arg1	1-kestose					627:635	1-kestose	627:635	1-kestose	627:635	Cultures were supplemented with different inulin-type fructans (1-kestose, Actilight, P95, Synergy1 and Inulin) and a galactooligosaccharide.
32019174	4	41	theme	different	595:603	arg1	Synergy1					654:661	Synergy1	654:661	Synergy1	654:661	Cultures were supplemented with different inulin-type fructans (1-kestose, Actilight, P95, Synergy1 and Inulin) and a galactooligosaccharide.
32019174	4	41	theme	different	595:603	arg1	Inulin					667:672	Inulin	667:672	Inulin	667:672	Cultures were supplemented with different inulin-type fructans (1-kestose, Actilight, P95, Synergy1 and Inulin) and a galactooligosaccharide.
32019174	4	41	theme	different	595:603	arg1	Actilight					638:646	Actilight	638:646	Actilight	638:646	Cultures were supplemented with different inulin-type fructans (1-kestose, Actilight, P95, Synergy1 and Inulin) and a galactooligosaccharide.
32019174	4	41	theme	different	595:603	arg1	P95					649:651	P95	649:651	P95	649:651	Cultures were supplemented with different inulin-type fructans (1-kestose, Actilight, P95, Synergy1 and Inulin) and a galactooligosaccharide.
32019174	4	41	theme	different	595:603	arg1	fructans					617:624	different inulin-type fructans	595:624	different inulin-type fructans (1-kestose, Actilight, P95, Synergy1 and Inulin)	595:673	Cultures were supplemented with different inulin-type fructans (1-kestose, Actilight, P95, Synergy1 and Inulin) and a galactooligosaccharide.
32019174	0	42	theme	Normal-Weight	125:137	arg1	Subjects					139:146	Morbid Obese and Normal-Weight Subjects	108:146	Morbid Obese and Normal-Weight Subjects	108:146	In Vitro Evaluation of Different Prebiotics on the Modulation of Gut Microbiota Composition and Function in Morbid Obese and Normal-Weight Subjects.
32019174	0	43	theme	Different	23:31	arg1	Prebiotics					33:42	Different Prebiotics	23:42	Different Prebiotics	23:42	In Vitro Evaluation of Different Prebiotics on the Modulation of Gut Microbiota Composition and Function in Morbid Obese and Normal-Weight Subjects.
32019174	10	44	from	differences	1511:1521	arg1	responses					1530:1538	the responses	1526:1538	the responses	1526:1538	Notably, the results obtained showed differences in the responses among the tested compounds but also among the studied human populations, indicating the need for developing population-specific products.
32019174	3	45	with	cultures	486:493	arg1	microbiota					500:509	microbiota	500:509	microbiota from healthy normal-weight and morbid obese adults	500:560	Herein, we carried out fecal cultures with microbiota from healthy normal-weight and morbid obese adults.
32019174	9	46	from	Faecalibacterium	1324:1339	arg1	subjects					1350:1357	obese subjects	1344:1357	obese subjects	1344:1357	The modulation with prebiotics led to significant increases in the Bacteroides group and Faecalibacterium in obese subjects, whereas in normal-weight individuals, substantial rises in Bifidobacterium and Faecalibacterium were appreciated.
32019174	7	47	theme	cell	1156:1159	arg1	line					1161:1164	an intestinal cell line	1142:1164	an intestinal cell line	1142:1164	Moreover, the functionality of the microbiota before and after prebiotic-modulation was determined in an in vitro model of interaction with an intestinal cell line.
32019174	8	48	theme	largest	1218:1224	arg1	effects					1226:1232	the largest effects	1214:1232	the largest effects	1214:1232	In general, 1-kestose was the compound showing the largest effects.
32019174	0	49	from	Modulation	51:60	arg1	Subjects					139:146	Morbid Obese and Normal-Weight Subjects	108:146	Morbid Obese and Normal-Weight Subjects	108:146	In Vitro Evaluation of Different Prebiotics on the Modulation of Gut Microbiota Composition and Function in Morbid Obese and Normal-Weight Subjects.
32019174	7	50	theme	microbiota	1037:1046	arg1	functionality					1016:1028	the functionality	1012:1028	the functionality of the microbiota before and after prebiotic-modulation	1012:1084	Moreover, the functionality of the microbiota before and after prebiotic-modulation was determined in an in vitro model of interaction with an intestinal cell line.
32019174	2	51	theme	best-suited	349:359	arg1	prebiotics					361:370	best-suited prebiotics	349:370	best-suited prebiotics	349:370	Its restoration towards a healthy condition using best-suited prebiotics requires previous development of in vitro models for evaluating their functionality.
32019174	6	52	theme	bifidobacterial	947:961	arg1	species					963:969	the bifidobacterial species	943:969	the bifidobacterial species	943:969	Additionally, the effect on the bifidobacterial species was assessed (ITS-sequencing).
32019174	5	53	theme	gut	725:727	arg1	microbiota					729:738	the gut microbiota	721:738	the gut microbiota	721:738	Their impact on the gut microbiota was assessed by monitoring gas production and evaluating changes in the microbiota composition (qPCR and 16S rRNA gene profiling) and metabolic activity (gas chromatography).
32019174	5	54	from	changes	797:803	arg1	composition					823:833	the microbiota composition	808:833	the microbiota composition (qPCR and 16S rRNA gene profiling)	808:868	Their impact on the gut microbiota was assessed by monitoring gas production and evaluating changes in the microbiota composition (qPCR and 16S rRNA gene profiling) and metabolic activity (gas chromatography).
32019174	5	54	from	changes	797:803	arg1	activity					884:891	metabolic activity	874:891	metabolic activity (gas chromatography)	874:912	Their impact on the gut microbiota was assessed by monitoring gas production and evaluating changes in the microbiota composition (qPCR and 16S rRNA gene profiling) and metabolic activity (gas chromatography).
32019174	5	54	from	changes	797:803	arg1	chromatography					898:911	gas chromatography	894:911	gas chromatography	894:911	Their impact on the gut microbiota was assessed by monitoring gas production and evaluating changes in the microbiota composition (qPCR and 16S rRNA gene profiling) and metabolic activity (gas chromatography).
32019174	9	55	theme	significant	1273:1283	arg1	increases					1285:1293	significant increases	1273:1293	significant increases in the Bacteroides group and Faecalibacterium in obese subjects	1273:1357	The modulation with prebiotics led to significant increases in the Bacteroides group and Faecalibacterium in obese subjects, whereas in normal-weight individuals, substantial rises in Bifidobacterium and Faecalibacterium were appreciated.
32019174	9	56	theme	Bacteroides	1302:1312	arg1	group					1314:1318	the Bacteroides group	1298:1318	the Bacteroides group	1298:1318	The modulation with prebiotics led to significant increases in the Bacteroides group and Faecalibacterium in obese subjects, whereas in normal-weight individuals, substantial rises in Bifidobacterium and Faecalibacterium were appreciated.
32019174	3	57	theme	healthy	516:522	arg1	adults					555:560	healthy normal-weight and morbid obese adults	516:560	healthy normal-weight and morbid obese adults	516:560	Herein, we carried out fecal cultures with microbiota from healthy normal-weight and morbid obese adults.
32019174	4	58	dep	fructans	617:624	arg1	1-kestose					627:635	1-kestose	627:635	1-kestose	627:635	Cultures were supplemented with different inulin-type fructans (1-kestose, Actilight, P95, Synergy1 and Inulin) and a galactooligosaccharide.
32019174	4	58	dep	fructans	617:624	arg1	Synergy1					654:661	Synergy1	654:661	Synergy1	654:661	Cultures were supplemented with different inulin-type fructans (1-kestose, Actilight, P95, Synergy1 and Inulin) and a galactooligosaccharide.
32019174	4	58	dep	fructans	617:624	arg1	Inulin					667:672	Inulin	667:672	Inulin	667:672	Cultures were supplemented with different inulin-type fructans (1-kestose, Actilight, P95, Synergy1 and Inulin) and a galactooligosaccharide.
32019174	4	58	dep	fructans	617:624	arg1	Actilight					638:646	Actilight	638:646	Actilight	638:646	Cultures were supplemented with different inulin-type fructans (1-kestose, Actilight, P95, Synergy1 and Inulin) and a galactooligosaccharide.
32019174	4	58	dep	fructans	617:624	arg1	P95					649:651	P95	649:651	P95	649:651	Cultures were supplemented with different inulin-type fructans (1-kestose, Actilight, P95, Synergy1 and Inulin) and a galactooligosaccharide.
32019174	4	58	dep	fructans	617:624	arg1	fructans					617:624	different inulin-type fructans	595:624	different inulin-type fructans (1-kestose, Actilight, P95, Synergy1 and Inulin)	595:673	Cultures were supplemented with different inulin-type fructans (1-kestose, Actilight, P95, Synergy1 and Inulin) and a galactooligosaccharide.
32019174	6	59	dep	assessed	975:982	arg1	ITS-sequencing					985:998	ITS-sequencing	985:998	ITS-sequencing	985:998	Additionally, the effect on the bifidobacterial species was assessed (ITS-sequencing).
32019174	2	60	dep	in	405:406	arg1	vitro					408:412	vitro	408:412	vitro	408:412	Its restoration towards a healthy condition using best-suited prebiotics requires previous development of in vitro models for evaluating their functionality.
32019174	9	61	from	Bifidobacterium	1419:1433	arg1	substantial					1398:1408	substantial	1398:1408	substantial	1398:1408	The modulation with prebiotics led to significant increases in the Bacteroides group and Faecalibacterium in obese subjects, whereas in normal-weight individuals, substantial rises in Bifidobacterium and Faecalibacterium were appreciated.
32019174	7	62	theme	interaction	1125:1135	arg1	model					1116:1120	an in vitro model	1104:1120	an in vitro model of interaction with an intestinal cell line	1104:1164	Moreover, the functionality of the microbiota before and after prebiotic-modulation was determined in an in vitro model of interaction with an intestinal cell line.
32019174	2	63	theme	healthy	325:331	arg1	condition					333:341	a healthy condition	323:341	a healthy condition using best-suited prebiotics	323:370	Its restoration towards a healthy condition using best-suited prebiotics requires previous development of in vitro models for evaluating their functionality.
32019174	1	64	theme	certain	221:227	arg1	factors					257:263	certain intrinsic and environmental factors	221:263	certain intrinsic and environmental factors	221:263	The gut microbiota remains relatively stable during adulthood; however, certain intrinsic and environmental factors can lead to microbiota dysbiosis.
32019174	9	65	from	increases	1285:1293	arg1	group					1314:1318	the Bacteroides group	1298:1318	the Bacteroides group	1298:1318	The modulation with prebiotics led to significant increases in the Bacteroides group and Faecalibacterium in obese subjects, whereas in normal-weight individuals, substantial rises in Bifidobacterium and Faecalibacterium were appreciated.
32019174	9	65	from	increases	1285:1293	arg1	Faecalibacterium					1324:1339	Faecalibacterium	1324:1339	Faecalibacterium in obese subjects	1324:1357	The modulation with prebiotics led to significant increases in the Bacteroides group and Faecalibacterium in obese subjects, whereas in normal-weight individuals, substantial rises in Bifidobacterium and Faecalibacterium were appreciated.
32019174	5	66	theme	gene	854:857	arg1	profiling					859:867	gene profiling	854:867	gene profiling	854:867	Their impact on the gut microbiota was assessed by monitoring gas production and evaluating changes in the microbiota composition (qPCR and 16S rRNA gene profiling) and metabolic activity (gas chromatography).
32019174	3	67	theme	morbid	542:547	arg1	adults					555:560	healthy normal-weight and morbid obese adults	516:560	healthy normal-weight and morbid obese adults	516:560	Herein, we carried out fecal cultures with microbiota from healthy normal-weight and morbid obese adults.
32019174	9	68	theme	normal-weight	1371:1383	arg1	individuals					1385:1395	normal-weight individuals	1371:1395	normal-weight individuals	1371:1395	The modulation with prebiotics led to significant increases in the Bacteroides group and Faecalibacterium in obese subjects, whereas in normal-weight individuals, substantial rises in Bifidobacterium and Faecalibacterium were appreciated.
32019174	1	69	theme	intrinsic	229:237	arg1	factors					257:263	certain intrinsic and environmental factors	221:263	certain intrinsic and environmental factors	221:263	The gut microbiota remains relatively stable during adulthood; however, certain intrinsic and environmental factors can lead to microbiota dysbiosis.
32019174	10	70	theme	human	1594:1598	arg1	populations					1600:1610	the studied human populations	1582:1610	the studied human populations	1582:1610	Notably, the results obtained showed differences in the responses among the tested compounds but also among the studied human populations, indicating the need for developing population-specific products.
32019174	0	71	dep	In	0:1	arg1	Vitro					3:7	Vitro	3:7	Vitro	3:7	In Vitro Evaluation of Different Prebiotics on the Modulation of Gut Microbiota Composition and Function in Morbid Obese and Normal-Weight Subjects.
32019174	2	72	theme	previous	381:388	arg1	development					390:400	previous development	381:400	previous development of in vitro models	381:419	Its restoration towards a healthy condition using best-suited prebiotics requires previous development of in vitro models for evaluating their functionality.
32019174	3	73	from	adults	555:560	arg1	microbiota					500:509	microbiota	500:509	microbiota from healthy normal-weight and morbid obese adults	500:560	Herein, we carried out fecal cultures with microbiota from healthy normal-weight and morbid obese adults.
32019174	5	74	from	impact	711:716	arg1	microbiota					729:738	the gut microbiota	721:738	the gut microbiota	721:738	Their impact on the gut microbiota was assessed by monitoring gas production and evaluating changes in the microbiota composition (qPCR and 16S rRNA gene profiling) and metabolic activity (gas chromatography).
34320383	15	0	theme	writhing	1929:1936	arg1	inhibition					1846:1855	inhibition	1846:1855	inhibition of formalin-induced pain (late and early phase) and acetic acid-induced writhing	1846:1936	By oral administration to mice, the infusion showed a significant (p<0.05) dose-dependent reduction of carrageenan-induced inflammation and inhibition of formalin-induced pain (late and early phase) and acetic acid-induced writhing compared with the control.
34320383	15	0	theme	writhing	1929:1936	arg1	reduction					1796:1804	a significant (p<0.05) dose-dependent reduction	1758:1804	a significant (p<0.05) dose-dependent reduction of carrageenan-induced inflammation	1758:1840	By oral administration to mice, the infusion showed a significant (p<0.05) dose-dependent reduction of carrageenan-induced inflammation and inhibition of formalin-induced pain (late and early phase) and acetic acid-induced writhing compared with the control.
34320383	12	1	theme	Phenolic	1105:1112	arg1	profile					1114:1120	Phenolic profile	1105:1120	Phenolic profile by LC-DAD-ESI/MSn	1105:1138	Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid.
34320383	15	2	theme	significant	1760:1770	arg1	reduction					1796:1804	a significant (p<0.05) dose-dependent reduction	1758:1804	a significant (p<0.05) dose-dependent reduction of carrageenan-induced inflammation	1758:1840	By oral administration to mice, the infusion showed a significant (p<0.05) dose-dependent reduction of carrageenan-induced inflammation and inhibition of formalin-induced pain (late and early phase) and acetic acid-induced writhing compared with the control.
34320383	13	3	theme	identified	1523:1532	arg1	compounds					1534:1542	the identified compounds	1519:1542	the identified compounds	1519:1542	This acid was the major compound representing 54% of the total content of the identified compounds and an absolute content of 18 mg/g of extract.
34320383	15	4	theme	acetic	1909:1914	arg1	writhing					1929:1936	acetic acid-induced writhing	1909:1936	acetic acid-induced writhing	1909:1936	By oral administration to mice, the infusion showed a significant (p<0.05) dose-dependent reduction of carrageenan-induced inflammation and inhibition of formalin-induced pain (late and early phase) and acetic acid-induced writhing compared with the control.
34320383	11	5	dep	RESULTS	1005:1011	arg1	showed					1026:1031	showed	1026:1031	showed 2018 mg GAE/100g DW of phenolics and 1956 mg ECE/100g DW of flavonoids	1026:1102	RESULTS The infusion showed 2018 mg GAE/100g DW of phenolics and 1956 mg ECE/100g DW of flavonoids.
34320383	12	6	dep	compounds	1169:1177	arg1	derivative					1203:1212	syringic acid hexoside derivative	1180:1212	syringic acid hexoside derivative	1180:1212	Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid.
34320383	12	6	dep	compounds	1169:1177	arg1	kaempferol-O-glucuronide					1280:1303	kaempferol-O-glucuronide	1280:1303	kaempferol-O-glucuronide	1280:1303	Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid.
34320383	12	6	dep	compounds	1169:1177	arg1	hexoside					1414:1421	rosmarinic acid hexoside	1398:1421	rosmarinic acid hexoside	1398:1421	Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid.
34320383	12	6	dep	compounds	1169:1177	arg1	apigenin-O-diglucuronide					1306:1329	apigenin-O-diglucuronide	1306:1329	apigenin-O-diglucuronide	1306:1329	Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid.
34320383	12	6	dep	compounds	1169:1177	arg1	compounds					1169:1177	ten compounds	1165:1177	ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid	1165:1442	Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid.
34320383	12	6	dep	compounds	1169:1177	arg1	acid					1365:1368	4-O-caffeoylquinic acid	1346:1368	4-O-caffeoylquinic acid	1346:1368	Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid.
34320383	12	6	dep	compounds	1169:1177	arg1	kaempferol-O-diglucuronide					1215:1240	kaempferol-O-diglucuronide	1215:1240	kaempferol-O-diglucuronide	1215:1240	Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid.
34320383	12	6	dep	compounds	1169:1177	arg1	eriodictyol-O-glucuronide					1371:1395	eriodictyol-O-glucuronide	1371:1395	eriodictyol-O-glucuronide	1371:1395	Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid.
34320383	12	6	dep	compounds	1169:1177	arg1	kaempferol-O-deoxyhexoside-hexoside					1243:1277	kaempferol-O-deoxyhexoside-hexoside	1243:1277	kaempferol-O-deoxyhexoside-hexoside	1243:1277	Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid.
34320383	12	6	dep	compounds	1169:1177	arg1	acid					1439:1442	rosmarinic acid	1428:1442	rosmarinic acid	1428:1442	Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid.
34320383	12	6	dep	compounds	1169:1177	arg1	acid					1340:1343	caffeic acid	1332:1343	caffeic acid	1332:1343	Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid.
34320383	17	7	theme	aerial	2129:2134	arg1	parts					2136:2140	the aerial parts	2125:2140	the aerial parts of S. chudaei	2125:2154	CONCLUSION This study reveals, for the first time, that the infusion of the aerial parts of S. chudaei is not toxic in a single dose and has remarkable antioxidant, anti-inflammatory, and analgesic activities, supporting the use of this species in folk medicine.
34320383	6	8	theme	STUDY	322:326	arg1	AIM					311:313	AIM	311:313	AIM OF THE STUDY	311:326	AIM OF THE STUDY This work aimed to confirm scientifically the referred properties.
34320383	10	9	theme	acute	841:845	arg1	toxicity					847:854	acute toxicity	841:854	acute toxicity	841:854	Using mice, acute toxicity, anti-inflammatory by carrageenan-induced paw edema, and analgesic by acetic acid-induced writhing and formalin-induced pain activities were tested.
34320383	13	10	theme	major	1463:1467	arg1	acid					1450:1453	This acid	1445:1453	This acid	1445:1453	This acid was the major compound representing 54% of the total content of the identified compounds and an absolute content of 18 mg/g of extract.
34320383	13	10	theme	major	1463:1467	arg1	compound					1469:1476	the major compound	1459:1476	the major compound representing 54% of the total content of the identified compounds and an absolute content of 18 mg/g of extract	1459:1588	This acid was the major compound representing 54% of the total content of the identified compounds and an absolute content of 18 mg/g of extract.
34320383	12	11	theme	syringic	1180:1187	arg1	derivative					1203:1212	syringic acid hexoside derivative	1180:1212	syringic acid hexoside derivative	1180:1212	Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid.
34320383	12	11	theme	syringic	1180:1187	arg1	compounds					1169:1177	ten compounds	1165:1177	ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid	1165:1442	Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid.
34320383	12	12	theme	rosmarinic	1428:1437	arg1	acid					1439:1442	rosmarinic acid	1428:1442	rosmarinic acid	1428:1442	Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid.
34320383	12	12	theme	rosmarinic	1428:1437	arg1	compounds					1169:1177	ten compounds	1165:1177	ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid	1165:1442	Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid.
34320383	17	13	dep	CONCLUSION	2053:2062	arg1	reveals					2075:2081	reveals	2075:2081	reveals	2075:2081	CONCLUSION This study reveals, for the first time, that the infusion of the aerial parts of S. chudaei is not toxic in a single dose and has remarkable antioxidant, anti-inflammatory, and analgesic activities, supporting the use of this species in folk medicine.
34320383	11	14	theme	GAE/100g	1041:1048	arg1	DW					1050:1051	2018 mg GAE/100g DW	1033:1051	2018 mg GAE/100g DW of phenolics	1033:1064	RESULTS The infusion showed 2018 mg GAE/100g DW of phenolics and 1956 mg ECE/100g DW of flavonoids.
34320383	7	15	theme	antioxidant	434:444	arg1	composition					418:428	the phenolic composition	405:428	the phenolic composition	405:428	For that, the phenolic composition and antioxidant activity were evaluated as well as acute toxicity, anti-inflammatory and analgesic effects of different doses of the infusion of S. chudaei aerial parts.
34320383	7	15	theme	antioxidant	434:444	arg1	activity					446:453	antioxidant activity	434:453	antioxidant activity	434:453	For that, the phenolic composition and antioxidant activity were evaluated as well as acute toxicity, anti-inflammatory and analgesic effects of different doses of the infusion of S. chudaei aerial parts.
34320383	7	15	theme	antioxidant	434:444	arg1	toxicity					487:494	acute toxicity	481:494	acute toxicity	481:494	For that, the phenolic composition and antioxidant activity were evaluated as well as acute toxicity, anti-inflammatory and analgesic effects of different doses of the infusion of S. chudaei aerial parts.
34320383	14	16	theme	81 μmol TE/g	1663:1674	arg1	DW					1676:1677	81 μmol TE/g DW	1663:1677	81 μmol TE/g DW	1663:1677	Additionally, the infusion exhibited a good antioxidant activity (DPPH: 81 μmol TE/g DW, FRAP: 438 μmol FSE/g DW).
34320383	17	17	theme	first	2092:2096	arg1	time					2098:2101	the first time	2088:2101	the first time	2088:2101	CONCLUSION This study reveals, for the first time, that the infusion of the aerial parts of S. chudaei is not toxic in a single dose and has remarkable antioxidant, anti-inflammatory, and analgesic activities, supporting the use of this species in folk medicine.
34320383	15	18	theme	carrageenan-induced	1809:1827	arg1	inflammation					1829:1840	carrageenan-induced inflammation	1809:1840	carrageenan-induced inflammation	1809:1840	By oral administration to mice, the infusion showed a significant (p<0.05) dose-dependent reduction of carrageenan-induced inflammation and inhibition of formalin-induced pain (late and early phase) and acetic acid-induced writhing compared with the control.
34320383	8	19	theme	MATERIALS	600:608	arg1	Infusion					622:629	MATERIALS AND METHODS Infusion	600:629	MATERIALS AND METHODS Infusion of aerial parts of S. chudaei	600:659	MATERIALS AND METHODS Infusion of aerial parts of S. chudaei was prepared and screened for phenolic composition by generalized methods TPC and TFC then by LC-DAD-ESI/MSn.
34320383	11	20	theme	ECE/100g	1078:1085	arg1	DW					1087:1088	1956 mg ECE/100g DW	1070:1088	1956 mg ECE/100g DW of flavonoids	1070:1102	RESULTS The infusion showed 2018 mg GAE/100g DW of phenolics and 1956 mg ECE/100g DW of flavonoids.
34320383	9	21	theme	antioxidant	807:817	arg1	activity					819:826	antioxidant activity	807:826	antioxidant activity	807:826	DPPH and FRAP were used to evaluate antioxidant activity.
34320383	8	22	theme	phenolic	691:698	arg1	composition					700:710	phenolic composition	691:710	phenolic composition	691:710	MATERIALS AND METHODS Infusion of aerial parts of S. chudaei was prepared and screened for phenolic composition by generalized methods TPC and TFC then by LC-DAD-ESI/MSn.
34320383	7	23	theme	phenolic	409:416	arg1	composition					418:428	the phenolic composition	405:428	the phenolic composition	405:428	For that, the phenolic composition and antioxidant activity were evaluated as well as acute toxicity, anti-inflammatory and analgesic effects of different doses of the infusion of S. chudaei aerial parts.
34320383	7	23	theme	phenolic	409:416	arg1	activity					446:453	antioxidant activity	434:453	antioxidant activity	434:453	For that, the phenolic composition and antioxidant activity were evaluated as well as acute toxicity, anti-inflammatory and analgesic effects of different doses of the infusion of S. chudaei aerial parts.
34320383	7	23	theme	phenolic	409:416	arg1	toxicity					487:494	acute toxicity	481:494	acute toxicity	481:494	For that, the phenolic composition and antioxidant activity were evaluated as well as acute toxicity, anti-inflammatory and analgesic effects of different doses of the infusion of S. chudaei aerial parts.
34320383	12	24	theme	acid	1189:1192	arg1	derivative					1203:1212	syringic acid hexoside derivative	1180:1212	syringic acid hexoside derivative	1180:1212	Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid.
34320383	12	24	theme	acid	1189:1192	arg1	compounds					1169:1177	ten compounds	1165:1177	ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid	1165:1442	Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid.
34320383	10	25	theme	acetic	926:931	arg1	writhing					946:953	acetic acid-induced writhing	926:953	acetic acid-induced writhing	926:953	Using mice, acute toxicity, anti-inflammatory by carrageenan-induced paw edema, and analgesic by acetic acid-induced writhing and formalin-induced pain activities were tested.
34320383	14	26	theme	good	1630:1633	arg1	DPPH					1657:1660	DPPH	1657:1660	DPPH	1657:1660	Additionally, the infusion exhibited a good antioxidant activity (DPPH: 81 μmol TE/g DW, FRAP: 438 μmol FSE/g DW).
34320383	14	26	theme	good	1630:1633	arg1	activity					1647:1654	a good antioxidant activity	1628:1654	a good antioxidant activity (DPPH: 81 μmol TE/g DW, FRAP: 438 μmol FSE/g DW)	1628:1703	Additionally, the infusion exhibited a good antioxidant activity (DPPH: 81 μmol TE/g DW, FRAP: 438 μmol FSE/g DW).
34320383	17	27	theme	remarkable	2194:2203	arg1	activities					2251:2260	remarkable antioxidant, anti-inflammatory, and analgesic activities	2194:2260	remarkable antioxidant, anti-inflammatory, and analgesic activities	2194:2260	CONCLUSION This study reveals, for the first time, that the infusion of the aerial parts of S. chudaei is not toxic in a single dose and has remarkable antioxidant, anti-inflammatory, and analgesic activities, supporting the use of this species in folk medicine.
34320383	15	28	theme	early	1892:1896	arg1	phase					1898:1902	early phase	1892:1902	early phase	1892:1902	By oral administration to mice, the infusion showed a significant (p<0.05) dose-dependent reduction of carrageenan-induced inflammation and inhibition of formalin-induced pain (late and early phase) and acetic acid-induced writhing compared with the control.
34320383	13	29	theme	extract	1582:1588	arg1	18 mg/g					1571:1577	18 mg/g	1571:1577	18 mg/g of extract	1571:1588	This acid was the major compound representing 54% of the total content of the identified compounds and an absolute content of 18 mg/g of extract.
34320383	10	30	theme	analgesic	913:921	arg1	activities					981:990	acute toxicity, anti-inflammatory by carrageenan-induced paw edema, and analgesic by acetic acid-induced writhing and formalin-induced pain activities	841:990	acute toxicity, anti-inflammatory by carrageenan-induced paw edema, and analgesic by acetic acid-induced writhing and formalin-induced pain activities	841:990	Using mice, acute toxicity, anti-inflammatory by carrageenan-induced paw edema, and analgesic by acetic acid-induced writhing and formalin-induced pain activities were tested.
34320383	12	31	theme	rosmarinic	1398:1407	arg1	compounds					1169:1177	ten compounds	1165:1177	ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid	1165:1442	Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid.
34320383	12	31	theme	rosmarinic	1398:1407	arg1	hexoside					1414:1421	rosmarinic acid hexoside	1398:1421	rosmarinic acid hexoside	1398:1421	Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid.
34320383	10	32	theme	anti-inflammatory	857:873	arg1	toxicity					847:854	acute toxicity	841:854	acute toxicity	841:854	Using mice, acute toxicity, anti-inflammatory by carrageenan-induced paw edema, and analgesic by acetic acid-induced writhing and formalin-induced pain activities were tested.
34320383	10	33	theme	formalin-induced	959:974	arg1	pain					976:979	formalin-induced pain	959:979	formalin-induced pain	959:979	Using mice, acute toxicity, anti-inflammatory by carrageenan-induced paw edema, and analgesic by acetic acid-induced writhing and formalin-induced pain activities were tested.
34320383	2	34	dep	characterization	113:128	arg1	Algeria					62:68	Algeria	62:68	Algeria	62:68	from Algeria: Chemical, toxicological and bioactivities characterization.
34320383	2	34	dep	characterization	113:128	arg1	bioactivities					99:111	bioactivities	99:111	bioactivities	99:111	from Algeria: Chemical, toxicological and bioactivities characterization.
34320383	15	35	theme	pain	1877:1880	arg1	inhibition					1846:1855	inhibition	1846:1855	inhibition of formalin-induced pain (late and early phase) and acetic acid-induced writhing	1846:1936	By oral administration to mice, the infusion showed a significant (p<0.05) dose-dependent reduction of carrageenan-induced inflammation and inhibition of formalin-induced pain (late and early phase) and acetic acid-induced writhing compared with the control.
34320383	15	35	theme	pain	1877:1880	arg1	reduction					1796:1804	a significant (p<0.05) dose-dependent reduction	1758:1804	a significant (p<0.05) dose-dependent reduction of carrageenan-induced inflammation	1758:1840	By oral administration to mice, the infusion showed a significant (p<0.05) dose-dependent reduction of carrageenan-induced inflammation and inhibition of formalin-induced pain (late and early phase) and acetic acid-induced writhing compared with the control.
34320383	6	36	dep	aimed	338:342	arg1	AIM					311:313	AIM	311:313	AIM OF THE STUDY	311:326	AIM OF THE STUDY This work aimed to confirm scientifically the referred properties.
34320383	0	37	theme	aerial	12:17	arg1	parts					19:23	aerial parts	12:23	aerial parts of Salvia chudaei Batt	12:46	Infusion of aerial parts of Salvia chudaei Batt.
34320383	7	38	theme	S.	575:576	arg1	parts					593:597	S. chudaei aerial parts	575:597	S. chudaei aerial parts	575:597	For that, the phenolic composition and antioxidant activity were evaluated as well as acute toxicity, anti-inflammatory and analgesic effects of different doses of the infusion of S. chudaei aerial parts.
34320383	8	39	theme	METHODS	614:620	arg1	Infusion					622:629	MATERIALS AND METHODS Infusion	600:629	MATERIALS AND METHODS Infusion of aerial parts of S. chudaei	600:659	MATERIALS AND METHODS Infusion of aerial parts of S. chudaei was prepared and screened for phenolic composition by generalized methods TPC and TFC then by LC-DAD-ESI/MSn.
34320383	17	40	theme	chudaei	2148:2154	arg1	parts					2136:2140	the aerial parts	2125:2140	the aerial parts of S. chudaei	2125:2154	CONCLUSION This study reveals, for the first time, that the infusion of the aerial parts of S. chudaei is not toxic in a single dose and has remarkable antioxidant, anti-inflammatory, and analgesic activities, supporting the use of this species in folk medicine.
34320383	13	41	theme	18 mg/g	1571:1577	arg1	18 mg/g					1571:1577	18 mg/g	1571:1577	18 mg/g of extract	1571:1588	This acid was the major compound representing 54% of the total content of the identified compounds and an absolute content of 18 mg/g of extract.
34320383	13	41	theme	18 mg/g	1571:1577	arg1	content					1560:1566	an absolute content	1548:1566	an absolute content of 18 mg/g of extract	1548:1588	This acid was the major compound representing 54% of the total content of the identified compounds and an absolute content of 18 mg/g of extract.
34320383	13	41	theme	18 mg/g	1571:1577	arg1	content					1508:1514	the total content	1498:1514	the total content of the identified compounds	1498:1542	This acid was the major compound representing 54% of the total content of the identified compounds and an absolute content of 18 mg/g of extract.
34320383	13	41	theme	18 mg/g	1571:1577	arg1	%					1493:1493	54%	1491:1493	54% of the total content of the identified compounds	1491:1542	This acid was the major compound representing 54% of the total content of the identified compounds and an absolute content of 18 mg/g of extract.
34320383	11	42	theme	flavonoids	1093:1102	arg1	DW					1050:1051	2018 mg GAE/100g DW	1033:1051	2018 mg GAE/100g DW of phenolics	1033:1064	RESULTS The infusion showed 2018 mg GAE/100g DW of phenolics and 1956 mg ECE/100g DW of flavonoids.
34320383	11	42	theme	flavonoids	1093:1102	arg1	DW					1087:1088	1956 mg ECE/100g DW	1070:1088	1956 mg ECE/100g DW of flavonoids	1070:1102	RESULTS The infusion showed 2018 mg GAE/100g DW of phenolics and 1956 mg ECE/100g DW of flavonoids.
34320383	5	43	theme	several	237:243	arg1	situations					299:308	inflammatory and pain-related situations	269:308	inflammatory and pain-related situations	269:308	from Algeria is traditionally used to relieve several dysfunctions, including inflammatory and pain-related situations.
34320383	5	43	theme	several	237:243	arg1	dysfunctions					245:256	several dysfunctions	237:256	several dysfunctions	237:256	from Algeria is traditionally used to relieve several dysfunctions, including inflammatory and pain-related situations.
34320383	7	44	theme	infusion	563:570	arg1	doses					550:554	different doses	540:554	different doses of the infusion of S. chudaei aerial parts	540:597	For that, the phenolic composition and antioxidant activity were evaluated as well as acute toxicity, anti-inflammatory and analgesic effects of different doses of the infusion of S. chudaei aerial parts.
34320383	14	45	dep	DPPH	1657:1660	arg1	FRAP					1680:1683	FRAP	1680:1683	FRAP	1680:1683	Additionally, the infusion exhibited a good antioxidant activity (DPPH: 81 μmol TE/g DW, FRAP: 438 μmol FSE/g DW).
34320383	14	45	dep	DPPH	1657:1660	arg1	DW					1676:1677	81 μmol TE/g DW	1663:1677	81 μmol TE/g DW	1663:1677	Additionally, the infusion exhibited a good antioxidant activity (DPPH: 81 μmol TE/g DW, FRAP: 438 μmol FSE/g DW).
34320383	14	45	dep	DPPH	1657:1660	arg1	DW					1701:1702	438 μmol FSE/g DW	1686:1702	438 μmol FSE/g DW	1686:1702	Additionally, the infusion exhibited a good antioxidant activity (DPPH: 81 μmol TE/g DW, FRAP: 438 μmol FSE/g DW).
34320383	13	46	theme	compounds	1534:1542	arg1	content					1508:1514	the total content	1498:1514	the total content of the identified compounds	1498:1542	This acid was the major compound representing 54% of the total content of the identified compounds and an absolute content of 18 mg/g of extract.
34320383	12	47	theme	caffeic	1332:1338	arg1	acid					1340:1343	caffeic acid	1332:1343	caffeic acid	1332:1343	Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid.
34320383	12	47	theme	caffeic	1332:1338	arg1	compounds					1169:1177	ten compounds	1165:1177	ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid	1165:1442	Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid.
34320383	8	48	theme	parts	641:645	arg1	Infusion					622:629	MATERIALS AND METHODS Infusion	600:629	MATERIALS AND METHODS Infusion of aerial parts of S. chudaei	600:659	MATERIALS AND METHODS Infusion of aerial parts of S. chudaei was prepared and screened for phenolic composition by generalized methods TPC and TFC then by LC-DAD-ESI/MSn.
34320383	15	49	theme	oral	1709:1712	arg1	administration					1714:1727	oral administration	1709:1727	oral administration to mice	1709:1735	By oral administration to mice, the infusion showed a significant (p<0.05) dose-dependent reduction of carrageenan-induced inflammation and inhibition of formalin-induced pain (late and early phase) and acetic acid-induced writhing compared with the control.
34320383	8	50	dep	methods	727:733	arg1	TPC					735:737	TPC	735:737	TPC	735:737	MATERIALS AND METHODS Infusion of aerial parts of S. chudaei was prepared and screened for phenolic composition by generalized methods TPC and TFC then by LC-DAD-ESI/MSn.
34320383	8	50	dep	methods	727:733	arg1	methods					727:733	generalized methods TPC and TFC	715:745	generalized methods TPC and TFC	715:745	MATERIALS AND METHODS Infusion of aerial parts of S. chudaei was prepared and screened for phenolic composition by generalized methods TPC and TFC then by LC-DAD-ESI/MSn.
34320383	8	50	dep	methods	727:733	arg1	TFC					743:745	TFC	743:745	TFC	743:745	MATERIALS AND METHODS Infusion of aerial parts of S. chudaei was prepared and screened for phenolic composition by generalized methods TPC and TFC then by LC-DAD-ESI/MSn.
34320383	7	51	theme	different	540:548	arg1	doses					550:554	different doses	540:554	different doses of the infusion of S. chudaei aerial parts	540:597	For that, the phenolic composition and antioxidant activity were evaluated as well as acute toxicity, anti-inflammatory and analgesic effects of different doses of the infusion of S. chudaei aerial parts.
34320383	17	52	from	toxic	2163:2167	arg1	dose					2181:2184	a single dose	2172:2184	a single dose	2172:2184	CONCLUSION This study reveals, for the first time, that the infusion of the aerial parts of S. chudaei is not toxic in a single dose and has remarkable antioxidant, anti-inflammatory, and analgesic activities, supporting the use of this species in folk medicine.
34320383	17	53	theme	single	2174:2179	arg1	dose					2181:2184	a single dose	2172:2184	a single dose	2172:2184	CONCLUSION This study reveals, for the first time, that the infusion of the aerial parts of S. chudaei is not toxic in a single dose and has remarkable antioxidant, anti-inflammatory, and analgesic activities, supporting the use of this species in folk medicine.
34320383	13	54	theme	content	1508:1514	arg1	18 mg/g					1571:1577	18 mg/g	1571:1577	18 mg/g of extract	1571:1588	This acid was the major compound representing 54% of the total content of the identified compounds and an absolute content of 18 mg/g of extract.
34320383	13	54	theme	content	1508:1514	arg1	content					1560:1566	an absolute content	1548:1566	an absolute content of 18 mg/g of extract	1548:1588	This acid was the major compound representing 54% of the total content of the identified compounds and an absolute content of 18 mg/g of extract.
34320383	13	54	theme	content	1508:1514	arg1	content					1508:1514	the total content	1498:1514	the total content of the identified compounds	1498:1542	This acid was the major compound representing 54% of the total content of the identified compounds and an absolute content of 18 mg/g of extract.
34320383	13	54	theme	content	1508:1514	arg1	%					1493:1493	54%	1491:1493	54% of the total content of the identified compounds	1491:1542	This acid was the major compound representing 54% of the total content of the identified compounds and an absolute content of 18 mg/g of extract.
34320383	5	55	theme	pain-related	286:297	arg1	situations					299:308	inflammatory and pain-related situations	269:308	inflammatory and pain-related situations	269:308	from Algeria is traditionally used to relieve several dysfunctions, including inflammatory and pain-related situations.
34320383	15	56	theme	acid-induced	1916:1927	arg1	writhing					1929:1936	acetic acid-induced writhing	1909:1936	acetic acid-induced writhing	1909:1936	By oral administration to mice, the infusion showed a significant (p<0.05) dose-dependent reduction of carrageenan-induced inflammation and inhibition of formalin-induced pain (late and early phase) and acetic acid-induced writhing compared with the control.
34320383	16	57	theme	8 g/kg	1999:2004	arg1	b.w.					2006:2009	8 g/kg b.w.	1999:2009	8 g/kg b.w.	1999:2009	On the other hand, infusion up to 8 g/kg b.w. showed no signs of toxicity or mortality.
34320383	7	58	theme	anti-inflammatory	497:513	arg1	effects					529:535	anti-inflammatory and analgesic effects	497:535	anti-inflammatory and analgesic effects of different doses of the infusion of S. chudaei aerial parts	497:597	For that, the phenolic composition and antioxidant activity were evaluated as well as acute toxicity, anti-inflammatory and analgesic effects of different doses of the infusion of S. chudaei aerial parts.
34320383	7	58	theme	anti-inflammatory	497:513	arg1	toxicity					487:494	acute toxicity	481:494	acute toxicity	481:494	For that, the phenolic composition and antioxidant activity were evaluated as well as acute toxicity, anti-inflammatory and analgesic effects of different doses of the infusion of S. chudaei aerial parts.
34320383	5	59	theme	inflammatory	269:280	arg1	situations					299:308	inflammatory and pain-related situations	269:308	inflammatory and pain-related situations	269:308	from Algeria is traditionally used to relieve several dysfunctions, including inflammatory and pain-related situations.
34320383	4	60	theme	&	183:183	arg1	Trab					185:188	& Trab	183:188	& Trab.	183:189	& Trab.
34320383	8	61	theme	generalized	715:725	arg1	TPC					735:737	TPC	735:737	TPC	735:737	MATERIALS AND METHODS Infusion of aerial parts of S. chudaei was prepared and screened for phenolic composition by generalized methods TPC and TFC then by LC-DAD-ESI/MSn.
34320383	8	61	theme	generalized	715:725	arg1	methods					727:733	generalized methods TPC and TFC	715:745	generalized methods TPC and TFC	715:745	MATERIALS AND METHODS Infusion of aerial parts of S. chudaei was prepared and screened for phenolic composition by generalized methods TPC and TFC then by LC-DAD-ESI/MSn.
34320383	8	61	theme	generalized	715:725	arg1	TFC					743:745	TFC	743:745	TFC	743:745	MATERIALS AND METHODS Infusion of aerial parts of S. chudaei was prepared and screened for phenolic composition by generalized methods TPC and TFC then by LC-DAD-ESI/MSn.
34320383	10	62	theme	toxicity	847:854	arg1	activities					981:990	acute toxicity, anti-inflammatory by carrageenan-induced paw edema, and analgesic by acetic acid-induced writhing and formalin-induced pain activities	841:990	acute toxicity, anti-inflammatory by carrageenan-induced paw edema, and analgesic by acetic acid-induced writhing and formalin-induced pain activities	841:990	Using mice, acute toxicity, anti-inflammatory by carrageenan-induced paw edema, and analgesic by acetic acid-induced writhing and formalin-induced pain activities were tested.
34320383	17	63	theme	species	2290:2296	arg1	use					2278:2280	the use	2274:2280	the use of this species in folk medicine	2274:2313	CONCLUSION This study reveals, for the first time, that the infusion of the aerial parts of S. chudaei is not toxic in a single dose and has remarkable antioxidant, anti-inflammatory, and analgesic activities, supporting the use of this species in folk medicine.
34320383	10	64	theme	paw	898:900	arg1	edema					902:906	carrageenan-induced paw edema	878:906	carrageenan-induced paw edema	878:906	Using mice, acute toxicity, anti-inflammatory by carrageenan-induced paw edema, and analgesic by acetic acid-induced writhing and formalin-induced pain activities were tested.
34320383	17	65	theme	parts	2136:2140	arg1	toxic					2163:2167	toxic	2163:2167	toxic	2163:2167	CONCLUSION This study reveals, for the first time, that the infusion of the aerial parts of S. chudaei is not toxic in a single dose and has remarkable antioxidant, anti-inflammatory, and analgesic activities, supporting the use of this species in folk medicine.
34320383	17	65	theme	parts	2136:2140	arg1	infusion					2113:2120	the infusion	2109:2120	the infusion of the aerial parts of S. chudaei	2109:2154	CONCLUSION This study reveals, for the first time, that the infusion of the aerial parts of S. chudaei is not toxic in a single dose and has remarkable antioxidant, anti-inflammatory, and analgesic activities, supporting the use of this species in folk medicine.
34320383	13	66	theme	total	1502:1506	arg1	content					1508:1514	the total content	1498:1514	the total content of the identified compounds	1498:1542	This acid was the major compound representing 54% of the total content of the identified compounds and an absolute content of 18 mg/g of extract.
34320383	17	67	theme	folk	2301:2304	arg1	medicine					2306:2313	folk medicine	2301:2313	folk medicine	2301:2313	CONCLUSION This study reveals, for the first time, that the infusion of the aerial parts of S. chudaei is not toxic in a single dose and has remarkable antioxidant, anti-inflammatory, and analgesic activities, supporting the use of this species in folk medicine.
34320383	7	68	theme	aerial	586:591	arg1	parts					593:597	S. chudaei aerial parts	575:597	S. chudaei aerial parts	575:597	For that, the phenolic composition and antioxidant activity were evaluated as well as acute toxicity, anti-inflammatory and analgesic effects of different doses of the infusion of S. chudaei aerial parts.
34320383	14	69	theme	438 μmol	1686:1693	arg1	DW					1701:1702	438 μmol FSE/g DW	1686:1702	438 μmol FSE/g DW	1686:1702	Additionally, the infusion exhibited a good antioxidant activity (DPPH: 81 μmol TE/g DW, FRAP: 438 μmol FSE/g DW).
34320383	10	70	theme	carrageenan-induced	878:896	arg1	edema					902:906	carrageenan-induced paw edema	878:906	carrageenan-induced paw edema	878:906	Using mice, acute toxicity, anti-inflammatory by carrageenan-induced paw edema, and analgesic by acetic acid-induced writhing and formalin-induced pain activities were tested.
34320383	9	71	used	used	790:793	arg2	FRAP					780:783	FRAP	780:783	FRAP	780:783	DPPH and FRAP were used to evaluate antioxidant activity.
34320383	9	71	used	used	790:793	arg2	DPPH					771:774	DPPH	771:774	DPPH	771:774	DPPH and FRAP were used to evaluate antioxidant activity.
34320383	15	72	theme	inflammation	1829:1840	arg1	inhibition					1846:1855	inhibition	1846:1855	inhibition of formalin-induced pain (late and early phase) and acetic acid-induced writhing	1846:1936	By oral administration to mice, the infusion showed a significant (p<0.05) dose-dependent reduction of carrageenan-induced inflammation and inhibition of formalin-induced pain (late and early phase) and acetic acid-induced writhing compared with the control.
34320383	15	72	theme	inflammation	1829:1840	arg1	reduction					1796:1804	a significant (p<0.05) dose-dependent reduction	1758:1804	a significant (p<0.05) dose-dependent reduction of carrageenan-induced inflammation	1758:1840	By oral administration to mice, the infusion showed a significant (p<0.05) dose-dependent reduction of carrageenan-induced inflammation and inhibition of formalin-induced pain (late and early phase) and acetic acid-induced writhing compared with the control.
34320383	11	73	theme	2018 mg	1033:1039	arg1	DW					1050:1051	2018 mg GAE/100g DW	1033:1051	2018 mg GAE/100g DW of phenolics	1033:1064	RESULTS The infusion showed 2018 mg GAE/100g DW of phenolics and 1956 mg ECE/100g DW of flavonoids.
34320383	16	74	theme	toxicity	2030:2037	arg1	signs					2021:2025	no signs	2018:2025	no signs of toxicity or mortality	2018:2050	On the other hand, infusion up to 8 g/kg b.w. showed no signs of toxicity or mortality.
34320383	3	75	theme	ETHNOPHARMACOLOGICAL	131:150	arg1	RELEVANCE					152:160	ETHNOPHARMACOLOGICAL RELEVANCE	131:160	ETHNOPHARMACOLOGICAL RELEVANCE	131:160	ETHNOPHARMACOLOGICAL RELEVANCE Salvia chudaei Batt.
34320383	3	76	dep	Batt	177:180	arg1	RELEVANCE					152:160	ETHNOPHARMACOLOGICAL RELEVANCE	131:160	ETHNOPHARMACOLOGICAL RELEVANCE	131:160	ETHNOPHARMACOLOGICAL RELEVANCE Salvia chudaei Batt.
34320383	16	77	theme	other	1972:1976	arg1	hand					1978:1981	the other hand	1968:1981	the other hand	1968:1981	On the other hand, infusion up to 8 g/kg b.w. showed no signs of toxicity or mortality.
34320383	11	78	theme	1956 mg	1070:1076	arg1	DW					1087:1088	1956 mg ECE/100g DW	1070:1088	1956 mg ECE/100g DW of flavonoids	1070:1102	RESULTS The infusion showed 2018 mg GAE/100g DW of phenolics and 1956 mg ECE/100g DW of flavonoids.
34320383	17	79	theme	analgesic	2241:2249	arg1	activities					2251:2260	remarkable antioxidant, anti-inflammatory, and analgesic activities	2194:2260	remarkable antioxidant, anti-inflammatory, and analgesic activities	2194:2260	CONCLUSION This study reveals, for the first time, that the infusion of the aerial parts of S. chudaei is not toxic in a single dose and has remarkable antioxidant, anti-inflammatory, and analgesic activities, supporting the use of this species in folk medicine.
34320383	17	80	from	use	2278:2280	arg1	medicine					2306:2313	folk medicine	2301:2313	folk medicine	2301:2313	CONCLUSION This study reveals, for the first time, that the infusion of the aerial parts of S. chudaei is not toxic in a single dose and has remarkable antioxidant, anti-inflammatory, and analgesic activities, supporting the use of this species in folk medicine.
34320383	15	81	dep	pain	1877:1880	arg1	late					1883:1886	late	1883:1886	late	1883:1886	By oral administration to mice, the infusion showed a significant (p<0.05) dose-dependent reduction of carrageenan-induced inflammation and inhibition of formalin-induced pain (late and early phase) and acetic acid-induced writhing compared with the control.
34320383	15	81	dep	pain	1877:1880	arg1	phase					1898:1902	early phase	1892:1902	early phase	1892:1902	By oral administration to mice, the infusion showed a significant (p<0.05) dose-dependent reduction of carrageenan-induced inflammation and inhibition of formalin-induced pain (late and early phase) and acetic acid-induced writhing compared with the control.
34320383	11	82	theme	phenolics	1056:1064	arg1	DW					1050:1051	2018 mg GAE/100g DW	1033:1051	2018 mg GAE/100g DW of phenolics	1033:1064	RESULTS The infusion showed 2018 mg GAE/100g DW of phenolics and 1956 mg ECE/100g DW of flavonoids.
34320383	11	82	theme	phenolics	1056:1064	arg1	DW					1087:1088	1956 mg ECE/100g DW	1070:1088	1956 mg ECE/100g DW of flavonoids	1070:1102	RESULTS The infusion showed 2018 mg GAE/100g DW of phenolics and 1956 mg ECE/100g DW of flavonoids.
34320383	2	83	dep	Chemical	71:78	arg1	toxicological					81:93	toxicological	81:93	toxicological	81:93	from Algeria: Chemical, toxicological and bioactivities characterization.
34320383	12	84	theme	hexoside	1194:1201	arg1	derivative					1203:1212	syringic acid hexoside derivative	1180:1212	syringic acid hexoside derivative	1180:1212	Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid.
34320383	12	84	theme	hexoside	1194:1201	arg1	compounds					1169:1177	ten compounds	1165:1177	ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid	1165:1442	Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid.
34320383	6	85	theme	referred	374:381	arg1	properties					383:392	the referred properties	370:392	the referred properties	370:392	AIM OF THE STUDY This work aimed to confirm scientifically the referred properties.
34320383	10	86	theme	acid-induced	933:944	arg1	writhing					946:953	acetic acid-induced writhing	926:953	acetic acid-induced writhing	926:953	Using mice, acute toxicity, anti-inflammatory by carrageenan-induced paw edema, and analgesic by acetic acid-induced writhing and formalin-induced pain activities were tested.
34320383	1	87	theme	&	49:49	arg1	Trab					51:54	& Trab	49:54	& Trab.	49:55	& Trab.
34320383	14	88	theme	antioxidant	1635:1645	arg1	DPPH					1657:1660	DPPH	1657:1660	DPPH	1657:1660	Additionally, the infusion exhibited a good antioxidant activity (DPPH: 81 μmol TE/g DW, FRAP: 438 μmol FSE/g DW).
34320383	14	88	theme	antioxidant	1635:1645	arg1	activity					1647:1654	a good antioxidant activity	1628:1654	a good antioxidant activity (DPPH: 81 μmol TE/g DW, FRAP: 438 μmol FSE/g DW)	1628:1703	Additionally, the infusion exhibited a good antioxidant activity (DPPH: 81 μmol TE/g DW, FRAP: 438 μmol FSE/g DW).
34320383	7	89	theme	acute	481:485	arg1	composition					418:428	the phenolic composition	405:428	the phenolic composition	405:428	For that, the phenolic composition and antioxidant activity were evaluated as well as acute toxicity, anti-inflammatory and analgesic effects of different doses of the infusion of S. chudaei aerial parts.
34320383	7	89	theme	acute	481:485	arg1	activity					446:453	antioxidant activity	434:453	antioxidant activity	434:453	For that, the phenolic composition and antioxidant activity were evaluated as well as acute toxicity, anti-inflammatory and analgesic effects of different doses of the infusion of S. chudaei aerial parts.
34320383	7	89	theme	acute	481:485	arg1	effects					529:535	anti-inflammatory and analgesic effects	497:535	anti-inflammatory and analgesic effects of different doses of the infusion of S. chudaei aerial parts	497:597	For that, the phenolic composition and antioxidant activity were evaluated as well as acute toxicity, anti-inflammatory and analgesic effects of different doses of the infusion of S. chudaei aerial parts.
34320383	7	89	theme	acute	481:485	arg1	toxicity					487:494	acute toxicity	481:494	acute toxicity	481:494	For that, the phenolic composition and antioxidant activity were evaluated as well as acute toxicity, anti-inflammatory and analgesic effects of different doses of the infusion of S. chudaei aerial parts.
34320383	7	90	theme	parts	593:597	arg1	infusion					563:570	the infusion	559:570	the infusion of S. chudaei aerial parts	559:597	For that, the phenolic composition and antioxidant activity were evaluated as well as acute toxicity, anti-inflammatory and analgesic effects of different doses of the infusion of S. chudaei aerial parts.
34320383	12	91	theme	acid	1409:1412	arg1	compounds					1169:1177	ten compounds	1165:1177	ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid	1165:1442	Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid.
34320383	12	91	theme	acid	1409:1412	arg1	hexoside					1414:1421	rosmarinic acid hexoside	1398:1421	rosmarinic acid hexoside	1398:1421	Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid.
34320383	17	92	theme	antioxidant	2205:2215	arg1	activities					2251:2260	remarkable antioxidant, anti-inflammatory, and analgesic activities	2194:2260	remarkable antioxidant, anti-inflammatory, and analgesic activities	2194:2260	CONCLUSION This study reveals, for the first time, that the infusion of the aerial parts of S. chudaei is not toxic in a single dose and has remarkable antioxidant, anti-inflammatory, and analgesic activities, supporting the use of this species in folk medicine.
34320383	12	93	theme	compounds	1169:1177	arg1	presence					1153:1160	the presence	1149:1160	the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid	1149:1442	Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid.
34320383	14	94	theme	FSE/g	1695:1699	arg1	DW					1701:1702	438 μmol FSE/g DW	1686:1702	438 μmol FSE/g DW	1686:1702	Additionally, the infusion exhibited a good antioxidant activity (DPPH: 81 μmol TE/g DW, FRAP: 438 μmol FSE/g DW).
34320383	7	95	theme	chudaei	578:584	arg1	parts					593:597	S. chudaei aerial parts	575:597	S. chudaei aerial parts	575:597	For that, the phenolic composition and antioxidant activity were evaluated as well as acute toxicity, anti-inflammatory and analgesic effects of different doses of the infusion of S. chudaei aerial parts.
34320383	17	96	theme	anti-inflammatory	2218:2234	arg1	activities					2251:2260	remarkable antioxidant, anti-inflammatory, and analgesic activities	2194:2260	remarkable antioxidant, anti-inflammatory, and analgesic activities	2194:2260	CONCLUSION This study reveals, for the first time, that the infusion of the aerial parts of S. chudaei is not toxic in a single dose and has remarkable antioxidant, anti-inflammatory, and analgesic activities, supporting the use of this species in folk medicine.
34320383	15	97	theme	formalin-induced	1860:1875	arg1	pain					1877:1880	formalin-induced pain	1860:1880	formalin-induced pain (late and early phase)	1860:1903	By oral administration to mice, the infusion showed a significant (p<0.05) dose-dependent reduction of carrageenan-induced inflammation and inhibition of formalin-induced pain (late and early phase) and acetic acid-induced writhing compared with the control.
34320383	0	98	theme	parts	19:23	arg1	Infusion					0:7	Infusion	0:7	Infusion of aerial parts of Salvia chudaei Batt.	0:47	Infusion of aerial parts of Salvia chudaei Batt.
34320383	17	99	contain	has	2190:2192	arg1	toxic					2163:2167	toxic	2163:2167	toxic	2163:2167	CONCLUSION This study reveals, for the first time, that the infusion of the aerial parts of S. chudaei is not toxic in a single dose and has remarkable antioxidant, anti-inflammatory, and analgesic activities, supporting the use of this species in folk medicine.
34320383	17	99	contain	has	2190:2192	arg1	infusion					2113:2120	the infusion	2109:2120	the infusion of the aerial parts of S. chudaei	2109:2154	CONCLUSION This study reveals, for the first time, that the infusion of the aerial parts of S. chudaei is not toxic in a single dose and has remarkable antioxidant, anti-inflammatory, and analgesic activities, supporting the use of this species in folk medicine.
34320383	17	99	contain	has	2190:2192	arg2	activities					2251:2260	remarkable antioxidant, anti-inflammatory, and analgesic activities	2194:2260	remarkable antioxidant, anti-inflammatory, and analgesic activities	2194:2260	CONCLUSION This study reveals, for the first time, that the infusion of the aerial parts of S. chudaei is not toxic in a single dose and has remarkable antioxidant, anti-inflammatory, and analgesic activities, supporting the use of this species in folk medicine.
34320383	16	100	theme	mortality	2042:2050	arg1	signs					2021:2025	no signs	2018:2025	no signs of toxicity or mortality	2018:2050	On the other hand, infusion up to 8 g/kg b.w. showed no signs of toxicity or mortality.
34320383	17	101	from	dose	2181:2184	arg1	toxic					2163:2167	toxic	2163:2167	toxic	2163:2167	CONCLUSION This study reveals, for the first time, that the infusion of the aerial parts of S. chudaei is not toxic in a single dose and has remarkable antioxidant, anti-inflammatory, and analgesic activities, supporting the use of this species in folk medicine.
34320383	17	101	from	dose	2181:2184	arg1	infusion					2113:2120	the infusion	2109:2120	the infusion of the aerial parts of S. chudaei	2109:2154	CONCLUSION This study reveals, for the first time, that the infusion of the aerial parts of S. chudaei is not toxic in a single dose and has remarkable antioxidant, anti-inflammatory, and analgesic activities, supporting the use of this species in folk medicine.
34320383	8	102	theme	aerial	634:639	arg1	parts					641:645	aerial parts	634:645	aerial parts of S. chudaei	634:659	MATERIALS AND METHODS Infusion of aerial parts of S. chudaei was prepared and screened for phenolic composition by generalized methods TPC and TFC then by LC-DAD-ESI/MSn.
34320383	0	103	theme	Batt	43:46	arg1	parts					19:23	aerial parts	12:23	aerial parts of Salvia chudaei Batt	12:46	Infusion of aerial parts of Salvia chudaei Batt.
34320383	7	104	theme	doses	550:554	arg1	effects					529:535	anti-inflammatory and analgesic effects	497:535	anti-inflammatory and analgesic effects of different doses of the infusion of S. chudaei aerial parts	497:597	For that, the phenolic composition and antioxidant activity were evaluated as well as acute toxicity, anti-inflammatory and analgesic effects of different doses of the infusion of S. chudaei aerial parts.
34320383	7	104	theme	doses	550:554	arg1	toxicity					487:494	acute toxicity	481:494	acute toxicity	481:494	For that, the phenolic composition and antioxidant activity were evaluated as well as acute toxicity, anti-inflammatory and analgesic effects of different doses of the infusion of S. chudaei aerial parts.
34320383	12	105	theme	4-O-caffeoylquinic	1346:1363	arg1	compounds					1169:1177	ten compounds	1165:1177	ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid	1165:1442	Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid.
34320383	12	105	theme	4-O-caffeoylquinic	1346:1363	arg1	acid					1365:1368	4-O-caffeoylquinic acid	1346:1368	4-O-caffeoylquinic acid	1346:1368	Phenolic profile by LC-DAD-ESI/MSn revealed the presence of ten compounds: syringic acid hexoside derivative, kaempferol-O-diglucuronide, kaempferol-O-deoxyhexoside-hexoside, kaempferol-O-glucuronide, apigenin-O-diglucuronide, caffeic acid, 4-O-caffeoylquinic acid, eriodictyol-O-glucuronide, rosmarinic acid hexoside, and rosmarinic acid.
34320383	8	106	theme	chudaei	653:659	arg1	parts					641:645	aerial parts	634:645	aerial parts of S. chudaei	634:659	MATERIALS AND METHODS Infusion of aerial parts of S. chudaei was prepared and screened for phenolic composition by generalized methods TPC and TFC then by LC-DAD-ESI/MSn.
34320383	13	107	theme	absolute	1551:1558	arg1	content					1560:1566	an absolute content	1548:1566	an absolute content of 18 mg/g of extract	1548:1588	This acid was the major compound representing 54% of the total content of the identified compounds and an absolute content of 18 mg/g of extract.
34320383	15	108	dep	significant	1760:1770	arg1	dose-dependent					1781:1794	dose-dependent	1781:1794	dose-dependent	1781:1794	By oral administration to mice, the infusion showed a significant (p<0.05) dose-dependent reduction of carrageenan-induced inflammation and inhibition of formalin-induced pain (late and early phase) and acetic acid-induced writhing compared with the control.
34320383	15	108	dep	significant	1760:1770	arg1	p<0.05					1773:1778	p<0.05	1773:1778	p<0.05	1773:1778	By oral administration to mice, the infusion showed a significant (p<0.05) dose-dependent reduction of carrageenan-induced inflammation and inhibition of formalin-induced pain (late and early phase) and acetic acid-induced writhing compared with the control.
34320383	7	109	theme	analgesic	519:527	arg1	effects					529:535	anti-inflammatory and analgesic effects	497:535	anti-inflammatory and analgesic effects of different doses of the infusion of S. chudaei aerial parts	497:597	For that, the phenolic composition and antioxidant activity were evaluated as well as acute toxicity, anti-inflammatory and analgesic effects of different doses of the infusion of S. chudaei aerial parts.
34320383	7	109	theme	analgesic	519:527	arg1	toxicity					487:494	acute toxicity	481:494	acute toxicity	481:494	For that, the phenolic composition and antioxidant activity were evaluated as well as acute toxicity, anti-inflammatory and analgesic effects of different doses of the infusion of S. chudaei aerial parts.
33894462	6	0	theme	microorganisms	1233:1246	arg1	release					1222:1228	the release	1218:1228	the release of microorganisms (p = 0.0001)	1218:1259	Data indicated that EM tool material composition impacted the release of microorganisms (p = 0.0001), where the PUF EM tool released TV more readily than the CELL EM tool.
33894462	2	1	theme	programs	355:362	arg1	effectiveness					327:339	effectiveness	327:339	effectiveness of sanitation programs	327:362	EM is used to determine harborage sites of microorganisms on processing equipment, assess effectiveness of sanitation programs, and prevent transmission of foodborne pathogens.
33894462	4	2	theme	operators	848:856	arg1	impact					830:835	the impact	826:835	the impact of various operators	826:856	Specifically, the objectives of this study were to 1) compare the ability of EM tools to release microorganisms into a recovery eluent, 2) characterize EM tool performance at decreasing inoculum concentrations, and 3) assess the impact of various operators during the processing of EM samples.
33894462	7	3	theme	inoculum	1359:1366	arg1	levels					1368:1373	the decreasing inoculum levels	1344:1373	the decreasing inoculum levels	1344:1373	Conversely, the decreasing inoculum levels did not statistically differ in the release of microorganisms from the EM tool matrices.
33894462	8	4	theme	human	1565:1569	arg1	operator					1571:1578	human operator	1565:1578	human operator	1565:1578	In addition, no significant difference was found between the machine stomacher and manual elution by human operator or between operators.
33894462	2	5	used	used	243:246	arg2	EM					237:238	EM	237:238	EM	237:238	EM is used to determine harborage sites of microorganisms on processing equipment, assess effectiveness of sanitation programs, and prevent transmission of foodborne pathogens.
33894462	2	6	theme	harborage	261:269	arg1	microorganisms					280:293	microorganisms	280:293	microorganisms	280:293	EM is used to determine harborage sites of microorganisms on processing equipment, assess effectiveness of sanitation programs, and prevent transmission of foodborne pathogens.
33894462	2	6	theme	harborage	261:269	arg1	sites					271:275	harborage sites	261:275	harborage sites of microorganisms on processing equipment	261:317	EM is used to determine harborage sites of microorganisms on processing equipment, assess effectiveness of sanitation programs, and prevent transmission of foodborne pathogens.
33894462	6	7	theme	material	1188:1195	arg1	composition					1197:1207	EM tool material composition	1180:1207	EM tool material composition	1180:1207	Data indicated that EM tool material composition impacted the release of microorganisms (p = 0.0001), where the PUF EM tool released TV more readily than the CELL EM tool.
33894462	1	8	theme	effective	188:196	arg1	program					228:234	an effective environmental monitoring (EM) program	185:234	an effective environmental monitoring (EM) program	185:234	The U.S. FDA Food Safety Modernization Act Preventive Controls for Human Food Rule underlines the importance of an effective environmental monitoring (EM) program.
33894462	4	9	theme	samples	886:892	arg1	processing					869:878	the processing	865:878	the processing of EM samples	865:892	Specifically, the objectives of this study were to 1) compare the ability of EM tools to release microorganisms into a recovery eluent, 2) characterize EM tool performance at decreasing inoculum concentrations, and 3) assess the impact of various operators during the processing of EM samples.
33894462	4	10	theme	study	638:642	arg1	objectives					619:628	the objectives	615:628	the objectives of this study	615:642	Specifically, the objectives of this study were to 1) compare the ability of EM tools to release microorganisms into a recovery eluent, 2) characterize EM tool performance at decreasing inoculum concentrations, and 3) assess the impact of various operators during the processing of EM samples.
33894462	6	11	theme	EM	1276:1277	arg1	tool					1279:1282	the PUF EM tool	1268:1282	the PUF EM tool	1268:1282	Data indicated that EM tool material composition impacted the release of microorganisms (p = 0.0001), where the PUF EM tool released TV more readily than the CELL EM tool.
33894462	3	12	theme	pathogens	563:571	arg1	release					542:548	the release	538:548	the release of foodborne pathogens from their sponge matrices	538:598	This study characterizes commercially-available polyurethane foam (PUF) and cellulose (CELL) EM tools for their efficacy in the release of foodborne pathogens from their sponge matrices.
33894462	1	13	theme	monitoring	212:221	arg1	program					228:234	an effective environmental monitoring (EM) program	185:234	an effective environmental monitoring (EM) program	185:234	The U.S. FDA Food Safety Modernization Act Preventive Controls for Human Food Rule underlines the importance of an effective environmental monitoring (EM) program.
33894462	9	14	used	used	1750:1753	arg2	differences					1706:1716	the differences	1702:1716	the differences identified in this study	1702:1741	Overall, the study provides a detailed characterization of two commercially-available EM tools, and the differences identified in this study can be used to improve the effectiveness of EM programs.
33894462	6	15	theme	tool	1183:1186	arg1	composition					1197:1207	EM tool material composition	1180:1207	EM tool material composition	1180:1207	Data indicated that EM tool material composition impacted the release of microorganisms (p = 0.0001), where the PUF EM tool released TV more readily than the CELL EM tool.
33894462	5	16	theme	decreasing	1028:1037	arg1	levels					1048:1053	decreasing inoculum levels	1028:1053	decreasing inoculum levels utilizing two elution techniques (mechanical stomacher, manually by operator)	1028:1131	Two bacteria (Listeria monocytogenes, Salmonella Typhimurium) and one human norovirus surrogate (Tulane virus [TV]) were compared at decreasing inoculum levels utilizing two elution techniques (mechanical stomacher, manually by operator), and across six operators.
33894462	1	17	theme	EM	224:225	arg1	program					228:234	an effective environmental monitoring (EM) program	185:234	an effective environmental monitoring (EM) program	185:234	The U.S. FDA Food Safety Modernization Act Preventive Controls for Human Food Rule underlines the importance of an effective environmental monitoring (EM) program.
33894462	2	18	from	sites	271:275	arg1	equipment					309:317	processing equipment	298:317	processing equipment	298:317	EM is used to determine harborage sites of microorganisms on processing equipment, assess effectiveness of sanitation programs, and prevent transmission of foodborne pathogens.
33894462	1	19	theme	Human	140:144	arg1	Rule					151:154	Human Food Rule	140:154	Human Food Rule	140:154	The U.S. FDA Food Safety Modernization Act Preventive Controls for Human Food Rule underlines the importance of an effective environmental monitoring (EM) program.
33894462	9	20	theme	EM	1688:1689	arg1	tools					1691:1695	two commercially-available EM tools	1661:1695	two commercially-available EM tools	1661:1695	Overall, the study provides a detailed characterization of two commercially-available EM tools, and the differences identified in this study can be used to improve the effectiveness of EM programs.
33894462	1	21	dep	Modernization	98:110	arg1	Act					112:114	Act	112:114	Act Preventive Controls for Human Food Rule	112:154	The U.S. FDA Food Safety Modernization Act Preventive Controls for Human Food Rule underlines the importance of an effective environmental monitoring (EM) program.
33894462	6	22	theme	CELL	1318:1321	arg1	tool					1326:1329	the CELL EM tool	1314:1329	the CELL EM tool	1314:1329	Data indicated that EM tool material composition impacted the release of microorganisms (p = 0.0001), where the PUF EM tool released TV more readily than the CELL EM tool.
33894462	4	23	dep	characterize	740:751	arg1	2					737:737	2	737:737	2	737:737	Specifically, the objectives of this study were to 1) compare the ability of EM tools to release microorganisms into a recovery eluent, 2) characterize EM tool performance at decreasing inoculum concentrations, and 3) assess the impact of various operators during the processing of EM samples.
33894462	8	24	theme	significant	1480:1490	arg1	difference					1492:1501	no significant difference	1477:1501	no significant difference	1477:1501	In addition, no significant difference was found between the machine stomacher and manual elution by human operator or between operators.
33894462	4	25	theme	tools	681:685	arg1	ability					667:673	the ability	663:673	the ability of EM tools to release microorganisms into a recovery eluent, 2) characterize EM tool performance at decreasing inoculum concentrations, and 3) assess the impact of various operators during the processing of EM samples	663:892	Specifically, the objectives of this study were to 1) compare the ability of EM tools to release microorganisms into a recovery eluent, 2) characterize EM tool performance at decreasing inoculum concentrations, and 3) assess the impact of various operators during the processing of EM samples.
33894462	5	26	theme	mechanical	1089:1098	arg1	stomacher					1100:1108	mechanical stomacher	1089:1108	mechanical stomacher	1089:1108	Two bacteria (Listeria monocytogenes, Salmonella Typhimurium) and one human norovirus surrogate (Tulane virus [TV]) were compared at decreasing inoculum levels utilizing two elution techniques (mechanical stomacher, manually by operator), and across six operators.
33894462	4	27	theme	EM	753:754	arg1	performance					761:771	EM tool performance	753:771	EM tool performance	753:771	Specifically, the objectives of this study were to 1) compare the ability of EM tools to release microorganisms into a recovery eluent, 2) characterize EM tool performance at decreasing inoculum concentrations, and 3) assess the impact of various operators during the processing of EM samples.
33894462	2	28	theme	pathogens	403:411	arg1	transmission					377:388	transmission	377:388	transmission of foodborne pathogens	377:411	EM is used to determine harborage sites of microorganisms on processing equipment, assess effectiveness of sanitation programs, and prevent transmission of foodborne pathogens.
33894462	1	29	theme	FDA	82:84	arg1	Modernization					98:110	The U.S. FDA Food Safety Modernization	73:110	The U.S. FDA Food Safety Modernization Act Preventive Controls for Human Food Rule	73:154	The U.S. FDA Food Safety Modernization Act Preventive Controls for Human Food Rule underlines the importance of an effective environmental monitoring (EM) program.
33894462	4	30	theme	recovery	720:727	arg1	eluent					729:734	a recovery eluent	718:734	a recovery eluent	718:734	Specifically, the objectives of this study were to 1) compare the ability of EM tools to release microorganisms into a recovery eluent, 2) characterize EM tool performance at decreasing inoculum concentrations, and 3) assess the impact of various operators during the processing of EM samples.
33894462	3	31	theme	commercially-available	439:460	arg1	PUF					481:483	commercially-available polyurethane foam (PUF) and cellulose (CELL) EM tools	439:514	PUF	481:483	This study characterizes commercially-available polyurethane foam (PUF) and cellulose (CELL) EM tools for their efficacy in the release of foodborne pathogens from their sponge matrices.
33894462	4	32	dep	assess	819:824	arg1	3					816:816	3	816:816	3	816:816	Specifically, the objectives of this study were to 1) compare the ability of EM tools to release microorganisms into a recovery eluent, 2) characterize EM tool performance at decreasing inoculum concentrations, and 3) assess the impact of various operators during the processing of EM samples.
33894462	1	33	theme	Safety	91:96	arg1	Modernization					98:110	The U.S. FDA Food Safety Modernization	73:110	The U.S. FDA Food Safety Modernization Act Preventive Controls for Human Food Rule	73:154	The U.S. FDA Food Safety Modernization Act Preventive Controls for Human Food Rule underlines the importance of an effective environmental monitoring (EM) program.
33894462	3	34	from	matrices	591:598	arg1	release					542:548	the release	538:548	the release of foodborne pathogens from their sponge matrices	538:598	This study characterizes commercially-available polyurethane foam (PUF) and cellulose (CELL) EM tools for their efficacy in the release of foodborne pathogens from their sponge matrices.
33894462	3	34	from	matrices	591:598	arg1	efficacy					526:533	their efficacy	520:533	their efficacy in the release of foodborne pathogens from their sponge matrices	520:598	This study characterizes commercially-available polyurethane foam (PUF) and cellulose (CELL) EM tools for their efficacy in the release of foodborne pathogens from their sponge matrices.
33894462	0	35	theme	microbial	18:26	arg1	release					28:34	microbial release	18:34	microbial release from environmental monitoring tools	18:70	Factors impacting microbial release from environmental monitoring tools.
33894462	7	36	theme	tool	1449:1452	arg1	matrices					1454:1461	the EM tool matrices	1442:1461	the EM tool matrices	1442:1461	Conversely, the decreasing inoculum levels did not statistically differ in the release of microorganisms from the EM tool matrices.
33894462	2	37	theme	sanitation	344:353	arg1	programs					355:362	sanitation programs	344:362	sanitation programs	344:362	EM is used to determine harborage sites of microorganisms on processing equipment, assess effectiveness of sanitation programs, and prevent transmission of foodborne pathogens.
33894462	0	38	theme	monitoring	55:64	arg1	tools					66:70	environmental monitoring tools	41:70	environmental monitoring tools	41:70	Factors impacting microbial release from environmental monitoring tools.
33894462	5	39	theme	virus	999:1003	arg1	[TV					1005:1007	Tulane virus [TV	992:1007	Tulane virus [TV	992:1007	Two bacteria (Listeria monocytogenes, Salmonella Typhimurium) and one human norovirus surrogate (Tulane virus [TV]) were compared at decreasing inoculum levels utilizing two elution techniques (mechanical stomacher, manually by operator), and across six operators.
33894462	5	39	theme	virus	999:1003	arg1	surrogate					981:989	one human norovirus surrogate	961:989	one human norovirus surrogate (Tulane virus [TV])	961:1009	Two bacteria (Listeria monocytogenes, Salmonella Typhimurium) and one human norovirus surrogate (Tulane virus [TV]) were compared at decreasing inoculum levels utilizing two elution techniques (mechanical stomacher, manually by operator), and across six operators.
33894462	7	40	theme	microorganisms	1422:1435	arg1	release					1411:1417	the release	1407:1417	the release of microorganisms from the EM tool matrices	1407:1461	Conversely, the decreasing inoculum levels did not statistically differ in the release of microorganisms from the EM tool matrices.
33894462	4	41	dep	compare	655:661	arg1	1					652:652	1	652:652	1	652:652	Specifically, the objectives of this study were to 1) compare the ability of EM tools to release microorganisms into a recovery eluent, 2) characterize EM tool performance at decreasing inoculum concentrations, and 3) assess the impact of various operators during the processing of EM samples.
33894462	9	42	theme	EM	1787:1788	arg1	programs					1790:1797	EM programs	1787:1797	EM programs	1787:1797	Overall, the study provides a detailed characterization of two commercially-available EM tools, and the differences identified in this study can be used to improve the effectiveness of EM programs.
33894462	5	43	theme	norovirus	971:979	arg1	[TV					1005:1007	Tulane virus [TV	992:1007	Tulane virus [TV	992:1007	Two bacteria (Listeria monocytogenes, Salmonella Typhimurium) and one human norovirus surrogate (Tulane virus [TV]) were compared at decreasing inoculum levels utilizing two elution techniques (mechanical stomacher, manually by operator), and across six operators.
33894462	5	43	theme	norovirus	971:979	arg1	surrogate					981:989	one human norovirus surrogate	961:989	one human norovirus surrogate (Tulane virus [TV])	961:1009	Two bacteria (Listeria monocytogenes, Salmonella Typhimurium) and one human norovirus surrogate (Tulane virus [TV]) were compared at decreasing inoculum levels utilizing two elution techniques (mechanical stomacher, manually by operator), and across six operators.
33894462	8	44	located	found	1507:1511	arg1	addition					1467:1474	addition	1467:1474	addition	1467:1474	In addition, no significant difference was found between the machine stomacher and manual elution by human operator or between operators.
33894462	8	44	located	found	1507:1511	arg2	difference					1492:1501	no significant difference	1477:1501	no significant difference	1477:1501	In addition, no significant difference was found between the machine stomacher and manual elution by human operator or between operators.
33894462	8	45	theme	manual	1547:1552	arg1	elution					1554:1560	manual elution	1547:1560	manual elution	1547:1560	In addition, no significant difference was found between the machine stomacher and manual elution by human operator or between operators.
33894462	2	46	theme	microorganisms	280:293	arg1	microorganisms					280:293	microorganisms	280:293	microorganisms	280:293	EM is used to determine harborage sites of microorganisms on processing equipment, assess effectiveness of sanitation programs, and prevent transmission of foodborne pathogens.
33894462	2	46	theme	microorganisms	280:293	arg1	sites					271:275	harborage sites	261:275	harborage sites of microorganisms on processing equipment	261:317	EM is used to determine harborage sites of microorganisms on processing equipment, assess effectiveness of sanitation programs, and prevent transmission of foodborne pathogens.
33894462	7	47	from	matrices	1454:1461	arg1	release					1411:1417	the release	1407:1417	the release of microorganisms from the EM tool matrices	1407:1461	Conversely, the decreasing inoculum levels did not statistically differ in the release of microorganisms from the EM tool matrices.
33894462	4	48	theme	various	840:846	arg1	operators					848:856	various operators	840:856	various operators	840:856	Specifically, the objectives of this study were to 1) compare the ability of EM tools to release microorganisms into a recovery eluent, 2) characterize EM tool performance at decreasing inoculum concentrations, and 3) assess the impact of various operators during the processing of EM samples.
33894462	5	49	dep	bacteria	899:906	arg1	monocytogenes					918:930	Listeria monocytogenes	909:930	Listeria monocytogenes	909:930	Two bacteria (Listeria monocytogenes, Salmonella Typhimurium) and one human norovirus surrogate (Tulane virus [TV]) were compared at decreasing inoculum levels utilizing two elution techniques (mechanical stomacher, manually by operator), and across six operators.
33894462	5	49	dep	bacteria	899:906	arg1	Typhimurium					944:954	Salmonella Typhimurium	933:954	Salmonella Typhimurium	933:954	Two bacteria (Listeria monocytogenes, Salmonella Typhimurium) and one human norovirus surrogate (Tulane virus [TV]) were compared at decreasing inoculum levels utilizing two elution techniques (mechanical stomacher, manually by operator), and across six operators.
33894462	3	50	from	efficacy	526:533	arg1	release					542:548	the release	538:548	the release of foodborne pathogens from their sponge matrices	538:598	This study characterizes commercially-available polyurethane foam (PUF) and cellulose (CELL) EM tools for their efficacy in the release of foodborne pathogens from their sponge matrices.
33894462	3	50	from	efficacy	526:533	arg1	matrices					591:598	their sponge matrices	578:598	their sponge matrices	578:598	This study characterizes commercially-available polyurethane foam (PUF) and cellulose (CELL) EM tools for their efficacy in the release of foodborne pathogens from their sponge matrices.
33894462	7	51	theme	decreasing	1348:1357	arg1	levels					1368:1373	the decreasing inoculum levels	1344:1373	the decreasing inoculum levels	1344:1373	Conversely, the decreasing inoculum levels did not statistically differ in the release of microorganisms from the EM tool matrices.
33894462	2	52	theme	processing	298:307	arg1	equipment					309:317	processing equipment	298:317	processing equipment	298:317	EM is used to determine harborage sites of microorganisms on processing equipment, assess effectiveness of sanitation programs, and prevent transmission of foodborne pathogens.
33894462	1	53	theme	environmental	198:210	arg1	program					228:234	an effective environmental monitoring (EM) program	185:234	an effective environmental monitoring (EM) program	185:234	The U.S. FDA Food Safety Modernization Act Preventive Controls for Human Food Rule underlines the importance of an effective environmental monitoring (EM) program.
33894462	4	54	theme	EM	883:884	arg1	samples					886:892	EM samples	883:892	EM samples	883:892	Specifically, the objectives of this study were to 1) compare the ability of EM tools to release microorganisms into a recovery eluent, 2) characterize EM tool performance at decreasing inoculum concentrations, and 3) assess the impact of various operators during the processing of EM samples.
33894462	6	55	theme	PUF	1272:1274	arg1	tool					1279:1282	the PUF EM tool	1268:1282	the PUF EM tool	1268:1282	Data indicated that EM tool material composition impacted the release of microorganisms (p = 0.0001), where the PUF EM tool released TV more readily than the CELL EM tool.
33894462	9	56	theme	detailed	1632:1639	arg1	characterization					1641:1656	a detailed characterization	1630:1656	a detailed characterization of two commercially-available EM tools	1630:1695	Overall, the study provides a detailed characterization of two commercially-available EM tools, and the differences identified in this study can be used to improve the effectiveness of EM programs.
33894462	6	57	theme	EM	1180:1181	arg1	composition					1197:1207	EM tool material composition	1180:1207	EM tool material composition	1180:1207	Data indicated that EM tool material composition impacted the release of microorganisms (p = 0.0001), where the PUF EM tool released TV more readily than the CELL EM tool.
33894462	5	58	theme	inoculum	1039:1046	arg1	levels					1048:1053	decreasing inoculum levels	1028:1053	decreasing inoculum levels utilizing two elution techniques (mechanical stomacher, manually by operator)	1028:1131	Two bacteria (Listeria monocytogenes, Salmonella Typhimurium) and one human norovirus surrogate (Tulane virus [TV]) were compared at decreasing inoculum levels utilizing two elution techniques (mechanical stomacher, manually by operator), and across six operators.
33894462	3	59	theme	sponge	584:589	arg1	matrices					591:598	their sponge matrices	578:598	their sponge matrices	578:598	This study characterizes commercially-available polyurethane foam (PUF) and cellulose (CELL) EM tools for their efficacy in the release of foodborne pathogens from their sponge matrices.
33894462	1	60	theme	Preventive	116:125	arg1	Controls					127:134	Preventive Controls	116:134	Preventive Controls	116:134	The U.S. FDA Food Safety Modernization Act Preventive Controls for Human Food Rule underlines the importance of an effective environmental monitoring (EM) program.
33894462	3	61	theme	foodborne	553:561	arg1	pathogens					563:571	foodborne pathogens	553:571	foodborne pathogens	553:571	This study characterizes commercially-available polyurethane foam (PUF) and cellulose (CELL) EM tools for their efficacy in the release of foodborne pathogens from their sponge matrices.
33894462	9	62	theme	commercially-available	1665:1686	arg1	tools					1691:1695	two commercially-available EM tools	1661:1695	two commercially-available EM tools	1661:1695	Overall, the study provides a detailed characterization of two commercially-available EM tools, and the differences identified in this study can be used to improve the effectiveness of EM programs.
33894462	6	63	theme	EM	1323:1324	arg1	tool					1326:1329	the CELL EM tool	1314:1329	the CELL EM tool	1314:1329	Data indicated that EM tool material composition impacted the release of microorganisms (p = 0.0001), where the PUF EM tool released TV more readily than the CELL EM tool.
33894462	1	64	theme	Food	146:149	arg1	Rule					151:154	Human Food Rule	140:154	Human Food Rule	140:154	The U.S. FDA Food Safety Modernization Act Preventive Controls for Human Food Rule underlines the importance of an effective environmental monitoring (EM) program.
33894462	9	65	theme	tools	1691:1695	arg1	characterization					1641:1656	a detailed characterization	1630:1656	a detailed characterization of two commercially-available EM tools	1630:1695	Overall, the study provides a detailed characterization of two commercially-available EM tools, and the differences identified in this study can be used to improve the effectiveness of EM programs.
33894462	4	66	theme	EM	678:679	arg1	tools					681:685	EM tools	678:685	EM tools	678:685	Specifically, the objectives of this study were to 1) compare the ability of EM tools to release microorganisms into a recovery eluent, 2) characterize EM tool performance at decreasing inoculum concentrations, and 3) assess the impact of various operators during the processing of EM samples.
33894462	0	67	from	tools	66:70	arg1	release					28:34	microbial release	18:34	microbial release from environmental monitoring tools	18:70	Factors impacting microbial release from environmental monitoring tools.
33894462	8	68	dep	stomacher	1533:1541	arg1	the					1521:1523	the	1521:1523	the	1521:1523	In addition, no significant difference was found between the machine stomacher and manual elution by human operator or between operators.
33894462	1	69	theme	U.S.	77:80	arg1	Modernization					98:110	The U.S. FDA Food Safety Modernization	73:110	The U.S. FDA Food Safety Modernization Act Preventive Controls for Human Food Rule	73:154	The U.S. FDA Food Safety Modernization Act Preventive Controls for Human Food Rule underlines the importance of an effective environmental monitoring (EM) program.
33894462	2	70	theme	foodborne	393:401	arg1	pathogens					403:411	foodborne pathogens	393:411	foodborne pathogens	393:411	EM is used to determine harborage sites of microorganisms on processing equipment, assess effectiveness of sanitation programs, and prevent transmission of foodborne pathogens.
33894462	5	71	theme	elution	1069:1075	arg1	techniques					1077:1086	two elution techniques	1065:1086	two elution techniques (mechanical stomacher, manually by operator)	1065:1131	Two bacteria (Listeria monocytogenes, Salmonella Typhimurium) and one human norovirus surrogate (Tulane virus [TV]) were compared at decreasing inoculum levels utilizing two elution techniques (mechanical stomacher, manually by operator), and across six operators.
33894462	1	72	theme	Food	86:89	arg1	Modernization					98:110	The U.S. FDA Food Safety Modernization	73:110	The U.S. FDA Food Safety Modernization Act Preventive Controls for Human Food Rule	73:154	The U.S. FDA Food Safety Modernization Act Preventive Controls for Human Food Rule underlines the importance of an effective environmental monitoring (EM) program.
33894462	3	73	theme	polyurethane	462:473	arg1	PUF					481:483	commercially-available polyurethane foam (PUF) and cellulose (CELL) EM tools	439:514	PUF	481:483	This study characterizes commercially-available polyurethane foam (PUF) and cellulose (CELL) EM tools for their efficacy in the release of foodborne pathogens from their sponge matrices.
33894462	5	74	theme	Listeria	909:916	arg1	monocytogenes					918:930	Listeria monocytogenes	909:930	Listeria monocytogenes	909:930	Two bacteria (Listeria monocytogenes, Salmonella Typhimurium) and one human norovirus surrogate (Tulane virus [TV]) were compared at decreasing inoculum levels utilizing two elution techniques (mechanical stomacher, manually by operator), and across six operators.
33894462	5	74	theme	Listeria	909:916	arg1	Typhimurium					944:954	Salmonella Typhimurium	933:954	Salmonella Typhimurium	933:954	Two bacteria (Listeria monocytogenes, Salmonella Typhimurium) and one human norovirus surrogate (Tulane virus [TV]) were compared at decreasing inoculum levels utilizing two elution techniques (mechanical stomacher, manually by operator), and across six operators.
33894462	5	75	dep	techniques	1077:1086	arg1	stomacher					1100:1108	mechanical stomacher	1089:1108	mechanical stomacher	1089:1108	Two bacteria (Listeria monocytogenes, Salmonella Typhimurium) and one human norovirus surrogate (Tulane virus [TV]) were compared at decreasing inoculum levels utilizing two elution techniques (mechanical stomacher, manually by operator), and across six operators.
33894462	3	76	theme	cellulose	490:498	arg1	tools					510:514	commercially-available polyurethane foam (PUF) and cellulose (CELL) EM tools	439:514	tools	510:514	This study characterizes commercially-available polyurethane foam (PUF) and cellulose (CELL) EM tools for their efficacy in the release of foodborne pathogens from their sponge matrices.
33894462	0	77	theme	environmental	41:53	arg1	tools					66:70	environmental monitoring tools	41:70	environmental monitoring tools	41:70	Factors impacting microbial release from environmental monitoring tools.
33894462	3	78	theme	foam	475:478	arg1	PUF					481:483	commercially-available polyurethane foam (PUF) and cellulose (CELL) EM tools	439:514	PUF	481:483	This study characterizes commercially-available polyurethane foam (PUF) and cellulose (CELL) EM tools for their efficacy in the release of foodborne pathogens from their sponge matrices.
33894462	4	79	theme	inoculum	787:794	arg1	concentrations					796:809	inoculum concentrations	787:809	inoculum concentrations	787:809	Specifically, the objectives of this study were to 1) compare the ability of EM tools to release microorganisms into a recovery eluent, 2) characterize EM tool performance at decreasing inoculum concentrations, and 3) assess the impact of various operators during the processing of EM samples.
33894462	5	80	theme	Tulane	992:997	arg1	[TV					1005:1007	Tulane virus [TV	992:1007	Tulane virus [TV	992:1007	Two bacteria (Listeria monocytogenes, Salmonella Typhimurium) and one human norovirus surrogate (Tulane virus [TV]) were compared at decreasing inoculum levels utilizing two elution techniques (mechanical stomacher, manually by operator), and across six operators.
33894462	5	80	theme	Tulane	992:997	arg1	surrogate					981:989	one human norovirus surrogate	961:989	one human norovirus surrogate (Tulane virus [TV])	961:1009	Two bacteria (Listeria monocytogenes, Salmonella Typhimurium) and one human norovirus surrogate (Tulane virus [TV]) were compared at decreasing inoculum levels utilizing two elution techniques (mechanical stomacher, manually by operator), and across six operators.
33894462	3	81	theme	EM	507:508	arg1	tools					510:514	commercially-available polyurethane foam (PUF) and cellulose (CELL) EM tools	439:514	tools	510:514	This study characterizes commercially-available polyurethane foam (PUF) and cellulose (CELL) EM tools for their efficacy in the release of foodborne pathogens from their sponge matrices.
33894462	7	82	theme	EM	1446:1447	arg1	matrices					1454:1461	the EM tool matrices	1442:1461	the EM tool matrices	1442:1461	Conversely, the decreasing inoculum levels did not statistically differ in the release of microorganisms from the EM tool matrices.
33894462	8	83	theme	machine	1525:1531	arg1	stomacher					1533:1541	machine stomacher	1525:1541	machine stomacher	1525:1541	In addition, no significant difference was found between the machine stomacher and manual elution by human operator or between operators.
33894462	4	84	theme	tool	756:759	arg1	performance					761:771	EM tool performance	753:771	EM tool performance	753:771	Specifically, the objectives of this study were to 1) compare the ability of EM tools to release microorganisms into a recovery eluent, 2) characterize EM tool performance at decreasing inoculum concentrations, and 3) assess the impact of various operators during the processing of EM samples.
33894462	3	85	theme	CELL	501:504	arg1	tools					510:514	commercially-available polyurethane foam (PUF) and cellulose (CELL) EM tools	439:514	tools	510:514	This study characterizes commercially-available polyurethane foam (PUF) and cellulose (CELL) EM tools for their efficacy in the release of foodborne pathogens from their sponge matrices.
33894462	1	86	theme	program	228:234	arg1	importance					171:180	the importance	167:180	the importance of an effective environmental monitoring (EM) program	167:234	The U.S. FDA Food Safety Modernization Act Preventive Controls for Human Food Rule underlines the importance of an effective environmental monitoring (EM) program.
33894462	5	87	theme	human	965:969	arg1	[TV					1005:1007	Tulane virus [TV	992:1007	Tulane virus [TV	992:1007	Two bacteria (Listeria monocytogenes, Salmonella Typhimurium) and one human norovirus surrogate (Tulane virus [TV]) were compared at decreasing inoculum levels utilizing two elution techniques (mechanical stomacher, manually by operator), and across six operators.
33894462	5	87	theme	human	965:969	arg1	surrogate					981:989	one human norovirus surrogate	961:989	one human norovirus surrogate (Tulane virus [TV])	961:1009	Two bacteria (Listeria monocytogenes, Salmonella Typhimurium) and one human norovirus surrogate (Tulane virus [TV]) were compared at decreasing inoculum levels utilizing two elution techniques (mechanical stomacher, manually by operator), and across six operators.
33894462	9	88	theme	programs	1790:1797	arg1	effectiveness					1770:1782	the effectiveness	1766:1782	the effectiveness of EM programs	1766:1797	Overall, the study provides a detailed characterization of two commercially-available EM tools, and the differences identified in this study can be used to improve the effectiveness of EM programs.
32582202	0	0	theme	Bacteroides-Derived	100:118	arg1	LPS					120:122	Bacteroides-Derived LPS	100:122	Bacteroides-Derived LPS	100:122	Chlorogenic Acid Protects Against Indomethacin-Induced Inflammation and Mucosa Damage by Decreasing Bacteroides-Derived LPS.
32582202	7	1	theme	microbiota	1160:1169	arg1	program					1187:1193	the fecal microbiota transplantation program	1150:1193	the fecal microbiota transplantation program	1150:1193	Notably, mice who had been colonized with intestinal microbiota from CGA-treated or CGA-and-indomethacin-treated mice, through the fecal microbiota transplantation program, were protected from indomethacin-induced inflammation, growth of Bacteroides, and the accumulation of Bacteroides-derived LPS, in congruence with those who had been treated with CGA.
32582202	4	2	theme	indomethacin-induced	682:701	arg1	inflammation					703:714	indomethacin-induced inflammation	682:714	indomethacin-induced inflammation	682:714	Then, fecal microbiota transplantation was performed to explore the role of intestinal microbiota in indomethacin-induced inflammation.
32582202	9	3	theme	damage	1812:1817	arg1	prevention					1773:1782	the prevention	1769:1782	the prevention of gastrointestinal mucosal damage in patients treated with indomethacin	1769:1855	This newly identified mechanism broadens our knowledge of how CGA exerts protective effects on intestinal inflammation and provides strategies for the prevention of gastrointestinal mucosal damage in patients treated with indomethacin.
32582202	5	4	from	damage	776:781	arg1	mice					902:905	indomethacin-treated mice	881:905	indomethacin-treated mice	881:905	Results: CGA treatment protected against body weight loss, damage to intestinal morphology and integrity, inflammation, and alteration of microbiota composition in indomethacin-treated mice.
32582202	7	5	theme	transplantation	1171:1185	arg1	program					1187:1193	the fecal microbiota transplantation program	1150:1193	the fecal microbiota transplantation program	1150:1193	Notably, mice who had been colonized with intestinal microbiota from CGA-treated or CGA-and-indomethacin-treated mice, through the fecal microbiota transplantation program, were protected from indomethacin-induced inflammation, growth of Bacteroides, and the accumulation of Bacteroides-derived LPS, in congruence with those who had been treated with CGA.
32582202	1	6	theme	natural	163:169	arg1	polyphenol					181:190	a natural bioactive polyphenol	161:190	a natural bioactive polyphenol	161:190	Background: Chlorogenic acid (CGA), a natural bioactive polyphenol, exerts anti-inflammatory, antioxidant, and antibacterial effects that support the maintenance of intestinal health.
32582202	1	6	theme	natural	163:169	arg1	acid					149:152	Chlorogenic acid	137:152	Chlorogenic acid (CGA)	137:158	Background: Chlorogenic acid (CGA), a natural bioactive polyphenol, exerts anti-inflammatory, antioxidant, and antibacterial effects that support the maintenance of intestinal health.
32582202	5	7	theme	CGA	726:728	arg1	treatment					730:738	CGA treatment	726:738	CGA treatment	726:738	Results: CGA treatment protected against body weight loss, damage to intestinal morphology and integrity, inflammation, and alteration of microbiota composition in indomethacin-treated mice.
32582202	8	8	theme	LPS	1584:1586	arg1	growth					1518:1523	the growth	1514:1523	the growth of Bacteroides	1514:1538	Conclusion: The results suggest that CGA may protect intestine integrity and alleviate inflammatory responses, primarily by inhibiting the growth of Bacteroides and the accumulation of Bacteroides-derived LPS, in indomethacin-induced colitis.
32582202	8	8	theme	LPS	1584:1586	arg1	accumulation					1548:1559	the accumulation	1544:1559	the accumulation of Bacteroides-derived LPS	1544:1586	Conclusion: The results suggest that CGA may protect intestine integrity and alleviate inflammatory responses, primarily by inhibiting the growth of Bacteroides and the accumulation of Bacteroides-derived LPS, in indomethacin-induced colitis.
32582202	2	9	from	influence	322:330	arg1	disease					459:465	inflammatory bowel disease	440:465	inflammatory bowel disease	440:465	However, the influence of CGA on gut microbiota and their metabolites, as well as its potential effects and mechanism of action in inflammatory bowel disease, remain to be elucidated.
32582202	2	9	from	influence	322:330	arg1	microbiota					346:355	gut microbiota	342:355	gut microbiota	342:355	However, the influence of CGA on gut microbiota and their metabolites, as well as its potential effects and mechanism of action in inflammatory bowel disease, remain to be elucidated.
32582202	8	10	theme	indomethacin-induced	1592:1611	arg1	colitis					1613:1619	indomethacin-induced colitis	1592:1619	indomethacin-induced colitis	1592:1619	Conclusion: The results suggest that CGA may protect intestine integrity and alleviate inflammatory responses, primarily by inhibiting the growth of Bacteroides and the accumulation of Bacteroides-derived LPS, in indomethacin-induced colitis.
32582202	7	11	theme	fecal	1154:1158	arg1	program					1187:1193	the fecal microbiota transplantation program	1150:1193	the fecal microbiota transplantation program	1150:1193	Notably, mice who had been colonized with intestinal microbiota from CGA-treated or CGA-and-indomethacin-treated mice, through the fecal microbiota transplantation program, were protected from indomethacin-induced inflammation, growth of Bacteroides, and the accumulation of Bacteroides-derived LPS, in congruence with those who had been treated with CGA.
32582202	7	12	theme	Bacteroides-derived	1298:1316	arg1	LPS					1318:1320	Bacteroides-derived LPS	1298:1320	Bacteroides-derived LPS	1298:1320	Notably, mice who had been colonized with intestinal microbiota from CGA-treated or CGA-and-indomethacin-treated mice, through the fecal microbiota transplantation program, were protected from indomethacin-induced inflammation, growth of Bacteroides, and the accumulation of Bacteroides-derived LPS, in congruence with those who had been treated with CGA.
32582202	5	13	theme	microbiota	855:864	arg1	composition					866:876	microbiota composition	855:876	microbiota composition	855:876	Results: CGA treatment protected against body weight loss, damage to intestinal morphology and integrity, inflammation, and alteration of microbiota composition in indomethacin-treated mice.
32582202	5	14	from	inflammation	823:834	arg1	mice					902:905	indomethacin-treated mice	881:905	indomethacin-treated mice	881:905	Results: CGA treatment protected against body weight loss, damage to intestinal morphology and integrity, inflammation, and alteration of microbiota composition in indomethacin-treated mice.
32582202	7	15	with	congruence	1326:1335	arg1	those					1342:1346	those	1342:1346	those	1342:1346	Notably, mice who had been colonized with intestinal microbiota from CGA-treated or CGA-and-indomethacin-treated mice, through the fecal microbiota transplantation program, were protected from indomethacin-induced inflammation, growth of Bacteroides, and the accumulation of Bacteroides-derived LPS, in congruence with those who had been treated with CGA.
32582202	9	16	theme	identified	1633:1642	arg1	mechanism					1644:1652	This newly identified mechanism	1622:1652	This newly identified mechanism	1622:1652	This newly identified mechanism broadens our knowledge of how CGA exerts protective effects on intestinal inflammation and provides strategies for the prevention of gastrointestinal mucosal damage in patients treated with indomethacin.
32582202	4	17	from	role	649:652	arg1	inflammation					703:714	indomethacin-induced inflammation	682:714	indomethacin-induced inflammation	682:714	Then, fecal microbiota transplantation was performed to explore the role of intestinal microbiota in indomethacin-induced inflammation.
32582202	1	18	theme	bioactive	171:179	arg1	polyphenol					181:190	a natural bioactive polyphenol	161:190	a natural bioactive polyphenol	161:190	Background: Chlorogenic acid (CGA), a natural bioactive polyphenol, exerts anti-inflammatory, antioxidant, and antibacterial effects that support the maintenance of intestinal health.
32582202	1	18	theme	bioactive	171:179	arg1	acid					149:152	Chlorogenic acid	137:152	Chlorogenic acid (CGA)	137:158	Background: Chlorogenic acid (CGA), a natural bioactive polyphenol, exerts anti-inflammatory, antioxidant, and antibacterial effects that support the maintenance of intestinal health.
32582202	3	19	theme	oral	512:515	arg1	gavage					517:522	an oral gavage	509:522	an oral gavage	509:522	Methods: First, an oral gavage was used to administer CGA to indomethacin-treated mice.
32582202	5	20	theme	composition	866:876	arg1	alteration					841:850	alteration	841:850	alteration of microbiota composition in indomethacin-treated mice	841:905	Results: CGA treatment protected against body weight loss, damage to intestinal morphology and integrity, inflammation, and alteration of microbiota composition in indomethacin-treated mice.
32582202	5	20	theme	composition	866:876	arg1	damage					776:781	damage	776:781	damage to intestinal morphology and integrity	776:820	Results: CGA treatment protected against body weight loss, damage to intestinal morphology and integrity, inflammation, and alteration of microbiota composition in indomethacin-treated mice.
32582202	5	20	theme	composition	866:876	arg1	loss					770:773	body weight loss	758:773	body weight loss	758:773	Results: CGA treatment protected against body weight loss, damage to intestinal morphology and integrity, inflammation, and alteration of microbiota composition in indomethacin-treated mice.
32582202	5	20	theme	composition	866:876	arg1	inflammation					823:834	inflammation	823:834	inflammation	823:834	Results: CGA treatment protected against body weight loss, damage to intestinal morphology and integrity, inflammation, and alteration of microbiota composition in indomethacin-treated mice.
32582202	2	21	theme	inflammatory	440:451	arg1	disease					459:465	inflammatory bowel disease	440:465	inflammatory bowel disease	440:465	However, the influence of CGA on gut microbiota and their metabolites, as well as its potential effects and mechanism of action in inflammatory bowel disease, remain to be elucidated.
32582202	7	22	theme	CGA-and-indomethacin-treated	1107:1134	arg1	mice					1136:1139	CGA-treated or CGA-and-indomethacin-treated mice	1092:1139	CGA-treated or CGA-and-indomethacin-treated mice	1092:1139	Notably, mice who had been colonized with intestinal microbiota from CGA-treated or CGA-and-indomethacin-treated mice, through the fecal microbiota transplantation program, were protected from indomethacin-induced inflammation, growth of Bacteroides, and the accumulation of Bacteroides-derived LPS, in congruence with those who had been treated with CGA.
32582202	0	23	theme	Chlorogenic	0:10	arg1	Acid					12:15	Chlorogenic Acid	0:15	Chlorogenic Acid	0:15	Chlorogenic Acid Protects Against Indomethacin-Induced Inflammation and Mucosa Damage by Decreasing Bacteroides-Derived LPS.
32582202	1	24	theme	intestinal	290:299	arg1	health					301:306	intestinal health	290:306	intestinal health	290:306	Background: Chlorogenic acid (CGA), a natural bioactive polyphenol, exerts anti-inflammatory, antioxidant, and antibacterial effects that support the maintenance of intestinal health.
32582202	9	25	theme	gastrointestinal	1787:1802	arg1	damage					1812:1817	gastrointestinal mucosal damage	1787:1817	gastrointestinal mucosal damage	1787:1817	This newly identified mechanism broadens our knowledge of how CGA exerts protective effects on intestinal inflammation and provides strategies for the prevention of gastrointestinal mucosal damage in patients treated with indomethacin.
32582202	7	26	theme	CGA-treated	1092:1102	arg1	mice					1136:1139	CGA-treated or CGA-and-indomethacin-treated mice	1092:1139	CGA-treated or CGA-and-indomethacin-treated mice	1092:1139	Notably, mice who had been colonized with intestinal microbiota from CGA-treated or CGA-and-indomethacin-treated mice, through the fecal microbiota transplantation program, were protected from indomethacin-induced inflammation, growth of Bacteroides, and the accumulation of Bacteroides-derived LPS, in congruence with those who had been treated with CGA.
32582202	5	27	theme	indomethacin-treated	881:900	arg1	mice					902:905	indomethacin-treated mice	881:905	indomethacin-treated mice	881:905	Results: CGA treatment protected against body weight loss, damage to intestinal morphology and integrity, inflammation, and alteration of microbiota composition in indomethacin-treated mice.
32582202	2	28	theme	action	430:435	arg1	metabolites					367:377	their metabolites	361:377	their metabolites	361:377	However, the influence of CGA on gut microbiota and their metabolites, as well as its potential effects and mechanism of action in inflammatory bowel disease, remain to be elucidated.
32582202	2	28	theme	action	430:435	arg1	mechanism					417:425	mechanism	417:425	mechanism	417:425	However, the influence of CGA on gut microbiota and their metabolites, as well as its potential effects and mechanism of action in inflammatory bowel disease, remain to be elucidated.
32582202	2	28	theme	action	430:435	arg1	influence					322:330	the influence	318:330	the influence of CGA on gut microbiota	318:355	However, the influence of CGA on gut microbiota and their metabolites, as well as its potential effects and mechanism of action in inflammatory bowel disease, remain to be elucidated.
32582202	2	28	theme	action	430:435	arg1	effects					405:411	its potential effects	391:411	its potential effects	391:411	However, the influence of CGA on gut microbiota and their metabolites, as well as its potential effects and mechanism of action in inflammatory bowel disease, remain to be elucidated.
32582202	1	29	theme	health	301:306	arg1	maintenance					275:285	the maintenance	271:285	the maintenance of intestinal health	271:306	Background: Chlorogenic acid (CGA), a natural bioactive polyphenol, exerts anti-inflammatory, antioxidant, and antibacterial effects that support the maintenance of intestinal health.
32582202	7	30	link	Bacteroides-derived	1298:1316	arg1	LPS					1318:1320	Bacteroides-derived LPS	1298:1320	Bacteroides-derived LPS	1298:1320	Notably, mice who had been colonized with intestinal microbiota from CGA-treated or CGA-and-indomethacin-treated mice, through the fecal microbiota transplantation program, were protected from indomethacin-induced inflammation, growth of Bacteroides, and the accumulation of Bacteroides-derived LPS, in congruence with those who had been treated with CGA.
32582202	2	31	from	metabolites	367:377	arg1	disease					459:465	inflammatory bowel disease	440:465	inflammatory bowel disease	440:465	However, the influence of CGA on gut microbiota and their metabolites, as well as its potential effects and mechanism of action in inflammatory bowel disease, remain to be elucidated.
32582202	2	31	from	metabolites	367:377	arg1	microbiota					346:355	gut microbiota	342:355	gut microbiota	342:355	However, the influence of CGA on gut microbiota and their metabolites, as well as its potential effects and mechanism of action in inflammatory bowel disease, remain to be elucidated.
32582202	5	32	theme	intestinal	786:795	arg1	morphology					797:806	morphology	797:806	morphology	797:806	Results: CGA treatment protected against body weight loss, damage to intestinal morphology and integrity, inflammation, and alteration of microbiota composition in indomethacin-treated mice.
32582202	1	33	theme	anti-inflammatory	200:216	arg1	effects					250:256	anti-inflammatory, antioxidant, and antibacterial effects that support the maintenance of intestinal health	200:306	anti-inflammatory, antioxidant, and antibacterial effects that support the maintenance of intestinal health	200:306	Background: Chlorogenic acid (CGA), a natural bioactive polyphenol, exerts anti-inflammatory, antioxidant, and antibacterial effects that support the maintenance of intestinal health.
32582202	7	34	theme	indomethacin-induced	1216:1235	arg1	inflammation					1237:1248	indomethacin-induced inflammation	1216:1248	indomethacin-induced inflammation	1216:1248	Notably, mice who had been colonized with intestinal microbiota from CGA-treated or CGA-and-indomethacin-treated mice, through the fecal microbiota transplantation program, were protected from indomethacin-induced inflammation, growth of Bacteroides, and the accumulation of Bacteroides-derived LPS, in congruence with those who had been treated with CGA.
32582202	6	35	from	integrity	979:987	arg1	mice					992:995	mice	992:995	mice treated with antibiotics	992:1020	Interestingly, CGA failed to inhibit inflammation or protect intestine integrity in mice treated with antibiotics.
32582202	7	36	theme	LPS	1318:1320	arg1	accumulation					1282:1293	the accumulation	1278:1293	the accumulation of Bacteroides-derived LPS	1278:1320	Notably, mice who had been colonized with intestinal microbiota from CGA-treated or CGA-and-indomethacin-treated mice, through the fecal microbiota transplantation program, were protected from indomethacin-induced inflammation, growth of Bacteroides, and the accumulation of Bacteroides-derived LPS, in congruence with those who had been treated with CGA.
32582202	7	36	theme	LPS	1318:1320	arg1	growth					1251:1256	growth	1251:1256	growth of Bacteroides	1251:1271	Notably, mice who had been colonized with intestinal microbiota from CGA-treated or CGA-and-indomethacin-treated mice, through the fecal microbiota transplantation program, were protected from indomethacin-induced inflammation, growth of Bacteroides, and the accumulation of Bacteroides-derived LPS, in congruence with those who had been treated with CGA.
32582202	7	36	theme	LPS	1318:1320	arg1	inflammation					1237:1248	indomethacin-induced inflammation	1216:1248	indomethacin-induced inflammation	1216:1248	Notably, mice who had been colonized with intestinal microbiota from CGA-treated or CGA-and-indomethacin-treated mice, through the fecal microbiota transplantation program, were protected from indomethacin-induced inflammation, growth of Bacteroides, and the accumulation of Bacteroides-derived LPS, in congruence with those who had been treated with CGA.
32582202	3	37	used	used	528:531	arg2	gavage					517:522	an oral gavage	509:522	an oral gavage	509:522	Methods: First, an oral gavage was used to administer CGA to indomethacin-treated mice.
32582202	9	38	from	prevention	1773:1782	arg1	patients					1822:1829	patients	1822:1829	patients treated with indomethacin	1822:1855	This newly identified mechanism broadens our knowledge of how CGA exerts protective effects on intestinal inflammation and provides strategies for the prevention of gastrointestinal mucosal damage in patients treated with indomethacin.
32582202	1	39	theme	antioxidant	219:229	arg1	effects					250:256	anti-inflammatory, antioxidant, and antibacterial effects that support the maintenance of intestinal health	200:306	anti-inflammatory, antioxidant, and antibacterial effects that support the maintenance of intestinal health	200:306	Background: Chlorogenic acid (CGA), a natural bioactive polyphenol, exerts anti-inflammatory, antioxidant, and antibacterial effects that support the maintenance of intestinal health.
32582202	0	40	theme	Indomethacin-Induced	34:53	arg1	Inflammation					55:66	Indomethacin-Induced Inflammation	34:66	Indomethacin-Induced Inflammation	34:66	Chlorogenic Acid Protects Against Indomethacin-Induced Inflammation and Mucosa Damage by Decreasing Bacteroides-Derived LPS.
32582202	6	41	theme	intestine	969:977	arg1	integrity					979:987	intestine integrity	969:987	intestine integrity in mice treated with antibiotics	969:1020	Interestingly, CGA failed to inhibit inflammation or protect intestine integrity in mice treated with antibiotics.
32582202	5	42	from	alteration	841:850	arg1	mice					902:905	indomethacin-treated mice	881:905	indomethacin-treated mice	881:905	Results: CGA treatment protected against body weight loss, damage to intestinal morphology and integrity, inflammation, and alteration of microbiota composition in indomethacin-treated mice.
32582202	2	43	theme	potential	395:403	arg1	effects					405:411	its potential effects	391:411	its potential effects	391:411	However, the influence of CGA on gut microbiota and their metabolites, as well as its potential effects and mechanism of action in inflammatory bowel disease, remain to be elucidated.
32582202	0	44	theme	Mucosa	72:77	arg1	Damage					79:84	Mucosa Damage	72:84	Mucosa Damage	72:84	Chlorogenic Acid Protects Against Indomethacin-Induced Inflammation and Mucosa Damage by Decreasing Bacteroides-Derived LPS.
32582202	7	45	theme	Bacteroides	1261:1271	arg1	accumulation					1282:1293	the accumulation	1278:1293	the accumulation of Bacteroides-derived LPS	1278:1320	Notably, mice who had been colonized with intestinal microbiota from CGA-treated or CGA-and-indomethacin-treated mice, through the fecal microbiota transplantation program, were protected from indomethacin-induced inflammation, growth of Bacteroides, and the accumulation of Bacteroides-derived LPS, in congruence with those who had been treated with CGA.
32582202	7	45	theme	Bacteroides	1261:1271	arg1	growth					1251:1256	growth	1251:1256	growth of Bacteroides	1251:1271	Notably, mice who had been colonized with intestinal microbiota from CGA-treated or CGA-and-indomethacin-treated mice, through the fecal microbiota transplantation program, were protected from indomethacin-induced inflammation, growth of Bacteroides, and the accumulation of Bacteroides-derived LPS, in congruence with those who had been treated with CGA.
32582202	7	45	theme	Bacteroides	1261:1271	arg1	inflammation					1237:1248	indomethacin-induced inflammation	1216:1248	indomethacin-induced inflammation	1216:1248	Notably, mice who had been colonized with intestinal microbiota from CGA-treated or CGA-and-indomethacin-treated mice, through the fecal microbiota transplantation program, were protected from indomethacin-induced inflammation, growth of Bacteroides, and the accumulation of Bacteroides-derived LPS, in congruence with those who had been treated with CGA.
32582202	7	46	from	mice	1136:1139	arg1	microbiota					1076:1085	intestinal microbiota	1065:1085	intestinal microbiota from CGA-treated or CGA-and-indomethacin-treated mice	1065:1139	Notably, mice who had been colonized with intestinal microbiota from CGA-treated or CGA-and-indomethacin-treated mice, through the fecal microbiota transplantation program, were protected from indomethacin-induced inflammation, growth of Bacteroides, and the accumulation of Bacteroides-derived LPS, in congruence with those who had been treated with CGA.
32582202	8	47	theme	inflammatory	1466:1477	arg1	responses					1479:1487	inflammatory responses	1466:1487	inflammatory responses	1466:1487	Conclusion: The results suggest that CGA may protect intestine integrity and alleviate inflammatory responses, primarily by inhibiting the growth of Bacteroides and the accumulation of Bacteroides-derived LPS, in indomethacin-induced colitis.
32582202	1	48	theme	Chlorogenic	137:147	arg1	CGA					155:157	CGA	155:157	CGA	155:157	Background: Chlorogenic acid (CGA), a natural bioactive polyphenol, exerts anti-inflammatory, antioxidant, and antibacterial effects that support the maintenance of intestinal health.
32582202	1	48	theme	Chlorogenic	137:147	arg1	acid					149:152	Chlorogenic acid	137:152	Chlorogenic acid (CGA)	137:158	Background: Chlorogenic acid (CGA), a natural bioactive polyphenol, exerts anti-inflammatory, antioxidant, and antibacterial effects that support the maintenance of intestinal health.
32582202	1	48	theme	Chlorogenic	137:147	arg1	polyphenol					181:190	a natural bioactive polyphenol	161:190	a natural bioactive polyphenol	161:190	Background: Chlorogenic acid (CGA), a natural bioactive polyphenol, exerts anti-inflammatory, antioxidant, and antibacterial effects that support the maintenance of intestinal health.
32582202	2	49	theme	bowel	453:457	arg1	disease					459:465	inflammatory bowel disease	440:465	inflammatory bowel disease	440:465	However, the influence of CGA on gut microbiota and their metabolites, as well as its potential effects and mechanism of action in inflammatory bowel disease, remain to be elucidated.
32582202	7	50	theme	intestinal	1065:1074	arg1	microbiota					1076:1085	intestinal microbiota	1065:1085	intestinal microbiota from CGA-treated or CGA-and-indomethacin-treated mice	1065:1139	Notably, mice who had been colonized with intestinal microbiota from CGA-treated or CGA-and-indomethacin-treated mice, through the fecal microbiota transplantation program, were protected from indomethacin-induced inflammation, growth of Bacteroides, and the accumulation of Bacteroides-derived LPS, in congruence with those who had been treated with CGA.
32582202	5	51	from	loss	770:773	arg1	mice					902:905	indomethacin-treated mice	881:905	indomethacin-treated mice	881:905	Results: CGA treatment protected against body weight loss, damage to intestinal morphology and integrity, inflammation, and alteration of microbiota composition in indomethacin-treated mice.
32582202	3	52	theme	indomethacin-treated	554:573	arg1	mice					575:578	indomethacin-treated mice	554:578	indomethacin-treated mice	554:578	Methods: First, an oral gavage was used to administer CGA to indomethacin-treated mice.
32582202	2	53	theme	gut	342:344	arg1	microbiota					346:355	gut microbiota	342:355	gut microbiota	342:355	However, the influence of CGA on gut microbiota and their metabolites, as well as its potential effects and mechanism of action in inflammatory bowel disease, remain to be elucidated.
32582202	9	54	theme	mucosal	1804:1810	arg1	damage					1812:1817	gastrointestinal mucosal damage	1787:1817	gastrointestinal mucosal damage	1787:1817	This newly identified mechanism broadens our knowledge of how CGA exerts protective effects on intestinal inflammation and provides strategies for the prevention of gastrointestinal mucosal damage in patients treated with indomethacin.
32582202	8	55	theme	Bacteroides	1528:1538	arg1	growth					1518:1523	the growth	1514:1523	the growth of Bacteroides	1514:1538	Conclusion: The results suggest that CGA may protect intestine integrity and alleviate inflammatory responses, primarily by inhibiting the growth of Bacteroides and the accumulation of Bacteroides-derived LPS, in indomethacin-induced colitis.
32582202	8	55	theme	Bacteroides	1528:1538	arg1	accumulation					1548:1559	the accumulation	1544:1559	the accumulation of Bacteroides-derived LPS	1544:1586	Conclusion: The results suggest that CGA may protect intestine integrity and alleviate inflammatory responses, primarily by inhibiting the growth of Bacteroides and the accumulation of Bacteroides-derived LPS, in indomethacin-induced colitis.
32582202	8	56	link	Bacteroides-derived	1564:1582	arg1	LPS					1584:1586	Bacteroides-derived LPS	1564:1586	Bacteroides-derived LPS	1564:1586	Conclusion: The results suggest that CGA may protect intestine integrity and alleviate inflammatory responses, primarily by inhibiting the growth of Bacteroides and the accumulation of Bacteroides-derived LPS, in indomethacin-induced colitis.
32582202	8	57	theme	intestine	1432:1440	arg1	integrity					1442:1450	intestine integrity	1432:1450	intestine integrity	1432:1450	Conclusion: The results suggest that CGA may protect intestine integrity and alleviate inflammatory responses, primarily by inhibiting the growth of Bacteroides and the accumulation of Bacteroides-derived LPS, in indomethacin-induced colitis.
32582202	2	58	from	effects	405:411	arg1	disease					459:465	inflammatory bowel disease	440:465	inflammatory bowel disease	440:465	However, the influence of CGA on gut microbiota and their metabolites, as well as its potential effects and mechanism of action in inflammatory bowel disease, remain to be elucidated.
32582202	2	58	from	effects	405:411	arg1	microbiota					346:355	gut microbiota	342:355	gut microbiota	342:355	However, the influence of CGA on gut microbiota and their metabolites, as well as its potential effects and mechanism of action in inflammatory bowel disease, remain to be elucidated.
32582202	2	59	theme	CGA	335:337	arg1	metabolites					367:377	their metabolites	361:377	their metabolites	361:377	However, the influence of CGA on gut microbiota and their metabolites, as well as its potential effects and mechanism of action in inflammatory bowel disease, remain to be elucidated.
32582202	2	59	theme	CGA	335:337	arg1	mechanism					417:425	mechanism	417:425	mechanism	417:425	However, the influence of CGA on gut microbiota and their metabolites, as well as its potential effects and mechanism of action in inflammatory bowel disease, remain to be elucidated.
32582202	2	59	theme	CGA	335:337	arg1	influence					322:330	the influence	318:330	the influence of CGA on gut microbiota	318:355	However, the influence of CGA on gut microbiota and their metabolites, as well as its potential effects and mechanism of action in inflammatory bowel disease, remain to be elucidated.
32582202	2	59	theme	CGA	335:337	arg1	effects					405:411	its potential effects	391:411	its potential effects	391:411	However, the influence of CGA on gut microbiota and their metabolites, as well as its potential effects and mechanism of action in inflammatory bowel disease, remain to be elucidated.
32582202	1	60	theme	antibacterial	236:248	arg1	effects					250:256	anti-inflammatory, antioxidant, and antibacterial effects that support the maintenance of intestinal health	200:306	anti-inflammatory, antioxidant, and antibacterial effects that support the maintenance of intestinal health	200:306	Background: Chlorogenic acid (CGA), a natural bioactive polyphenol, exerts anti-inflammatory, antioxidant, and antibacterial effects that support the maintenance of intestinal health.
32582202	5	61	theme	body	758:761	arg1	loss					770:773	body weight loss	758:773	body weight loss	758:773	Results: CGA treatment protected against body weight loss, damage to intestinal morphology and integrity, inflammation, and alteration of microbiota composition in indomethacin-treated mice.
32582202	9	62	theme	intestinal	1717:1726	arg1	inflammation					1728:1739	intestinal inflammation	1717:1739	intestinal inflammation	1717:1739	This newly identified mechanism broadens our knowledge of how CGA exerts protective effects on intestinal inflammation and provides strategies for the prevention of gastrointestinal mucosal damage in patients treated with indomethacin.
32582202	4	63	theme	microbiota	668:677	arg1	role					649:652	the role	645:652	the role of intestinal microbiota in indomethacin-induced inflammation	645:714	Then, fecal microbiota transplantation was performed to explore the role of intestinal microbiota in indomethacin-induced inflammation.
32582202	4	64	theme	microbiota	593:602	arg1	transplantation					604:618	fecal microbiota transplantation	587:618	fecal microbiota transplantation	587:618	Then, fecal microbiota transplantation was performed to explore the role of intestinal microbiota in indomethacin-induced inflammation.
32582202	5	65	theme	weight	763:768	arg1	loss					770:773	body weight loss	758:773	body weight loss	758:773	Results: CGA treatment protected against body weight loss, damage to intestinal morphology and integrity, inflammation, and alteration of microbiota composition in indomethacin-treated mice.
32582202	8	66	theme	Bacteroides-derived	1564:1582	arg1	LPS					1584:1586	Bacteroides-derived LPS	1564:1586	Bacteroides-derived LPS	1564:1586	Conclusion: The results suggest that CGA may protect intestine integrity and alleviate inflammatory responses, primarily by inhibiting the growth of Bacteroides and the accumulation of Bacteroides-derived LPS, in indomethacin-induced colitis.
32582202	2	67	from	mechanism	417:425	arg1	disease					459:465	inflammatory bowel disease	440:465	inflammatory bowel disease	440:465	However, the influence of CGA on gut microbiota and their metabolites, as well as its potential effects and mechanism of action in inflammatory bowel disease, remain to be elucidated.
32582202	2	67	from	mechanism	417:425	arg1	microbiota					346:355	gut microbiota	342:355	gut microbiota	342:355	However, the influence of CGA on gut microbiota and their metabolites, as well as its potential effects and mechanism of action in inflammatory bowel disease, remain to be elucidated.
32582202	4	68	theme	intestinal	657:666	arg1	microbiota					668:677	intestinal microbiota	657:677	intestinal microbiota	657:677	Then, fecal microbiota transplantation was performed to explore the role of intestinal microbiota in indomethacin-induced inflammation.
32582202	9	69	theme	protective	1695:1704	arg1	effects					1706:1712	protective effects	1695:1712	protective effects	1695:1712	This newly identified mechanism broadens our knowledge of how CGA exerts protective effects on intestinal inflammation and provides strategies for the prevention of gastrointestinal mucosal damage in patients treated with indomethacin.
32582202	4	70	theme	fecal	587:591	arg1	transplantation					604:618	fecal microbiota transplantation	587:618	fecal microbiota transplantation	587:618	Then, fecal microbiota transplantation was performed to explore the role of intestinal microbiota in indomethacin-induced inflammation.
33226209	0	0	theme	Antibacterial/Osteogenic	109:132	arg1	Properties					149:158	Shape Auto-Matched and Dual-Light-Defined Antibacterial/Osteogenic Pattern Switch Properties	67:158	Properties	149:158	Jelly-Inspired Injectable Guided Tissue Regeneration Strategy with Shape Auto-Matched and Dual-Light-Defined Antibacterial/Osteogenic Pattern Switch Properties.
33226209	4	1	theme	TiO2	761:764	arg1	PDA					766:768	TiO2@PDA	761:768	TiO2@PDA	761:768	This study proposed an injectable sodium alginate hydrogel composite (CTP-SA) doped with cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles for GTR.
33226209	4	1	theme	TiO2	761:764	arg1	dioxide					752:758	polydopamine-coated titanium dioxide	723:758	polydopamine-coated titanium dioxide (TiO2@PDA)	723:769	This study proposed an injectable sodium alginate hydrogel composite (CTP-SA) doped with cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles for GTR.
33226209	7	2	theme	oxygen	1154:1159	arg1	species					1161:1167	the reactive oxygen species	1141:1167	the reactive oxygen species released under BL excitation	1141:1196	Moreover, the reactive oxygen species released under BL excitation could accelerate the oxidation of Cu+ to Cu2+.
33226209	2	3	theme	antibacterial	421:433	arg1	properties					435:444	the limited antibacterial properties	409:444	the limited antibacterial properties	409:444	The current guided tissue regeneration (GTR) membranes for periodontitis treatments cannot effectively promote tissue regeneration for the limited antibacterial properties and the excessively fast degradation rate.
33226209	0	4	theme	Dual-Light-Defined	90:107	arg1	Properties					149:158	Shape Auto-Matched and Dual-Light-Defined Antibacterial/Osteogenic Pattern Switch Properties	67:158	Properties	149:158	Jelly-Inspired Injectable Guided Tissue Regeneration Strategy with Shape Auto-Matched and Dual-Light-Defined Antibacterial/Osteogenic Pattern Switch Properties.
33226209	6	5	theme	abundant	1094:1101	arg1	bacteria					1103:1110	the most abundant bacteria	1085:1110	the most abundant bacteria in oral biofilms	1085:1127	Meanwhile, CTP-SA exhibited broad-spectrum antibacterial capabilities under blue light (BL) irradiation, including Streptococcus mutans (one of the most abundant bacteria in oral biofilms).
33226209	9	6	theme	antibacterial	1534:1546	arg1	modes					1563:1567	antibacterial and osteogenic modes	1534:1567	antibacterial and osteogenic modes	1534:1567	Collectively, through this dual-light (blue and NIR) noninvasive regulation, CTP-SA could switch antibacterial and osteogenic modes to address requirements of patients at different healing stages, thereby realizing the customized GTR procedures.
33226209	2	7	theme	limited	413:419	arg1	properties					435:444	the limited antibacterial properties	409:444	the limited antibacterial properties	409:444	The current guided tissue regeneration (GTR) membranes for periodontitis treatments cannot effectively promote tissue regeneration for the limited antibacterial properties and the excessively fast degradation rate.
33226209	0	8	theme	Switch	142:147	arg1	Properties					149:158	Shape Auto-Matched and Dual-Light-Defined Antibacterial/Osteogenic Pattern Switch Properties	67:158	Properties	149:158	Jelly-Inspired Injectable Guided Tissue Regeneration Strategy with Shape Auto-Matched and Dual-Light-Defined Antibacterial/Osteogenic Pattern Switch Properties.
33226209	9	9	theme	GTR	1667:1669	arg1	procedures					1671:1680	the customized GTR procedures	1652:1680	the customized GTR procedures	1652:1680	Collectively, through this dual-light (blue and NIR) noninvasive regulation, CTP-SA could switch antibacterial and osteogenic modes to address requirements of patients at different healing stages, thereby realizing the customized GTR procedures.
33226209	5	10	dep	change	851:856	arg1	solid					869:873	solid	869:873	solid	869:873	Inspired by the gelation process of the jelly, the phase change (liquid to solid) of CTP-SA after injection could automatch variform bone defects.
33226209	6	11	from	bacteria	1103:1110	arg1	biofilms					1120:1127	oral biofilms	1115:1127	oral biofilms	1115:1127	Meanwhile, CTP-SA exhibited broad-spectrum antibacterial capabilities under blue light (BL) irradiation, including Streptococcus mutans (one of the most abundant bacteria in oral biofilms).
33226209	2	12	theme	periodontitis	333:345	arg1	treatments					347:356	periodontitis treatments	333:356	periodontitis treatments	333:356	The current guided tissue regeneration (GTR) membranes for periodontitis treatments cannot effectively promote tissue regeneration for the limited antibacterial properties and the excessively fast degradation rate.
33226209	4	13	theme	polydopamine-coated	723:741	arg1	PDA					766:768	TiO2@PDA	761:768	TiO2@PDA	761:768	This study proposed an injectable sodium alginate hydrogel composite (CTP-SA) doped with cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles for GTR.
33226209	4	13	theme	polydopamine-coated	723:741	arg1	dioxide					752:758	polydopamine-coated titanium dioxide	723:758	polydopamine-coated titanium dioxide (TiO2@PDA)	723:769	This study proposed an injectable sodium alginate hydrogel composite (CTP-SA) doped with cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles for GTR.
33226209	0	14	theme	Pattern	134:140	arg1	Properties					149:158	Shape Auto-Matched and Dual-Light-Defined Antibacterial/Osteogenic Pattern Switch Properties	67:158	Properties	149:158	Jelly-Inspired Injectable Guided Tissue Regeneration Strategy with Shape Auto-Matched and Dual-Light-Defined Antibacterial/Osteogenic Pattern Switch Properties.
33226209	6	15	dep	mutans	1070:1075	arg1	bacteria					1103:1110	the most abundant bacteria	1085:1110	the most abundant bacteria in oral biofilms	1085:1127	Meanwhile, CTP-SA exhibited broad-spectrum antibacterial capabilities under blue light (BL) irradiation, including Streptococcus mutans (one of the most abundant bacteria in oral biofilms).
33226209	6	15	dep	mutans	1070:1075	arg1	one					1078:1080	one	1078:1080	one	1078:1080	Meanwhile, CTP-SA exhibited broad-spectrum antibacterial capabilities under blue light (BL) irradiation, including Streptococcus mutans (one of the most abundant bacteria in oral biofilms).
33226209	7	16	theme	reactive	1145:1152	arg1	species					1161:1167	the reactive oxygen species	1141:1167	the reactive oxygen species released under BL excitation	1141:1196	Moreover, the reactive oxygen species released under BL excitation could accelerate the oxidation of Cu+ to Cu2+.
33226209	8	17	theme	photothermal	1368:1379	arg1	effect					1381:1386	the photothermal effect	1364:1386	the photothermal effect of CTP-SA under near-infrared (NIR) irradiation	1364:1434	Afterward, osteogenesis could be enhanced through two factors simultaneously: the stimulation of newly formed Cu2+ and the photothermal effect of CTP-SA under near-infrared (NIR) irradiation.
33226209	2	18	theme	tissue	385:390	arg1	regeneration					392:403	tissue regeneration	385:403	tissue regeneration	385:403	The current guided tissue regeneration (GTR) membranes for periodontitis treatments cannot effectively promote tissue regeneration for the limited antibacterial properties and the excessively fast degradation rate.
33226209	4	19	theme	oxide	706:710	arg1	nanoparticles					771:783	cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles	692:783	cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles for GTR	692:791	This study proposed an injectable sodium alginate hydrogel composite (CTP-SA) doped with cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles for GTR.
33226209	8	20	theme	CTP-SA	1391:1396	arg1	Cu2+					1355:1358	newly formed Cu2+	1342:1358	newly formed Cu2+	1342:1358	Afterward, osteogenesis could be enhanced through two factors simultaneously: the stimulation of newly formed Cu2+ and the photothermal effect of CTP-SA under near-infrared (NIR) irradiation.
33226209	8	20	theme	CTP-SA	1391:1396	arg1	effect					1381:1386	the photothermal effect	1364:1386	the photothermal effect of CTP-SA under near-infrared (NIR) irradiation	1364:1434	Afterward, osteogenesis could be enhanced through two factors simultaneously: the stimulation of newly formed Cu2+ and the photothermal effect of CTP-SA under near-infrared (NIR) irradiation.
33226209	2	21	theme	degradation	471:481	arg1	rate					483:486	the excessively fast degradation rate	450:486	the excessively fast degradation rate	450:486	The current guided tissue regeneration (GTR) membranes for periodontitis treatments cannot effectively promote tissue regeneration for the limited antibacterial properties and the excessively fast degradation rate.
33226209	7	22	theme	Cu+	1232:1234	arg1	oxidation					1219:1227	the oxidation	1215:1227	the oxidation of Cu+ to Cu2+	1215:1242	Moreover, the reactive oxygen species released under BL excitation could accelerate the oxidation of Cu+ to Cu2+.
33226209	8	23	theme	Cu2+	1355:1358	arg1	stimulation					1327:1337	the stimulation	1323:1337	the stimulation of newly formed Cu2+ and the photothermal effect of CTP-SA under near-infrared (NIR) irradiation	1323:1434	Afterward, osteogenesis could be enhanced through two factors simultaneously: the stimulation of newly formed Cu2+ and the photothermal effect of CTP-SA under near-infrared (NIR) irradiation.
33226209	7	24	theme	BL	1184:1185	arg1	excitation					1187:1196	BL excitation	1184:1196	BL excitation	1184:1196	Moreover, the reactive oxygen species released under BL excitation could accelerate the oxidation of Cu+ to Cu2+.
33226209	9	25	theme	patients	1596:1603	arg1	requirements					1580:1591	requirements	1580:1591	requirements of patients	1580:1603	Collectively, through this dual-light (blue and NIR) noninvasive regulation, CTP-SA could switch antibacterial and osteogenic modes to address requirements of patients at different healing stages, thereby realizing the customized GTR procedures.
33226209	2	26	theme	fast	466:469	arg1	rate					483:486	the excessively fast degradation rate	450:486	the excessively fast degradation rate	450:486	The current guided tissue regeneration (GTR) membranes for periodontitis treatments cannot effectively promote tissue regeneration for the limited antibacterial properties and the excessively fast degradation rate.
33226209	0	27	theme	Injectable	15:24	arg1	Strategy					53:60	Jelly-Inspired Injectable Guided Tissue Regeneration Strategy	0:60	Jelly-Inspired Injectable Guided Tissue Regeneration Strategy with Shape Auto-Matched and Dual-Light-Defined Antibacterial/Osteogenic Pattern Switch Properties.	0:159	Jelly-Inspired Injectable Guided Tissue Regeneration Strategy with Shape Auto-Matched and Dual-Light-Defined Antibacterial/Osteogenic Pattern Switch Properties.
33226209	6	28	theme	blue	1017:1020	arg1	light					1022:1026	blue light	1017:1026	blue light (BL) irradiation	1017:1043	Meanwhile, CTP-SA exhibited broad-spectrum antibacterial capabilities under blue light (BL) irradiation, including Streptococcus mutans (one of the most abundant bacteria in oral biofilms).
33226209	6	28	theme	blue	1017:1020	arg1	BL					1029:1030	BL	1029:1030	BL	1029:1030	Meanwhile, CTP-SA exhibited broad-spectrum antibacterial capabilities under blue light (BL) irradiation, including Streptococcus mutans (one of the most abundant bacteria in oral biofilms).
33226209	8	29	theme	near-infrared	1404:1416	arg1	irradiation					1424:1434	near-infrared (NIR) irradiation	1404:1434	near-infrared (NIR) irradiation	1404:1434	Afterward, osteogenesis could be enhanced through two factors simultaneously: the stimulation of newly formed Cu2+ and the photothermal effect of CTP-SA under near-infrared (NIR) irradiation.
33226209	8	29	theme	near-infrared	1404:1416	arg1	NIR					1419:1421	NIR	1419:1421	NIR	1419:1421	Afterward, osteogenesis could be enhanced through two factors simultaneously: the stimulation of newly formed Cu2+ and the photothermal effect of CTP-SA under near-infrared (NIR) irradiation.
33226209	4	30	theme	dioxide	752:758	arg1	nanoparticles					771:783	cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles	692:783	cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles for GTR	692:791	This study proposed an injectable sodium alginate hydrogel composite (CTP-SA) doped with cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles for GTR.
33226209	0	31	theme	Jelly-Inspired	0:13	arg1	Strategy					53:60	Jelly-Inspired Injectable Guided Tissue Regeneration Strategy	0:60	Jelly-Inspired Injectable Guided Tissue Regeneration Strategy with Shape Auto-Matched and Dual-Light-Defined Antibacterial/Osteogenic Pattern Switch Properties.	0:159	Jelly-Inspired Injectable Guided Tissue Regeneration Strategy with Shape Auto-Matched and Dual-Light-Defined Antibacterial/Osteogenic Pattern Switch Properties.
33226209	4	32	theme	sodium	637:642	arg1	CTP-SA					673:678	CTP-SA	673:678	CTP-SA	673:678	This study proposed an injectable sodium alginate hydrogel composite (CTP-SA) doped with cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles for GTR.
33226209	4	32	theme	sodium	637:642	arg1	composite					662:670	an injectable sodium alginate hydrogel composite	623:670	an injectable sodium alginate hydrogel composite (CTP-SA) doped with cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles for GTR	623:791	This study proposed an injectable sodium alginate hydrogel composite (CTP-SA) doped with cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles for GTR.
33226209	3	33	dep	imprecise	586:594	arg1	match					596:600	match	596:600	match	596:600	Besides, they need extra tailoring according to variform defects before implantation, leading to imprecise match.
33226209	1	34	theme	periodontal	232:242	arg1	tissues					255:261	periodontal supporting tissues	232:261	periodontal supporting tissues	232:261	Periodontitis is a bacterial infectious disease leading to the loss of periodontal supporting tissues and teeth.
33226209	0	35	theme	Tissue	33:38	arg1	Strategy					53:60	Jelly-Inspired Injectable Guided Tissue Regeneration Strategy	0:60	Jelly-Inspired Injectable Guided Tissue Regeneration Strategy with Shape Auto-Matched and Dual-Light-Defined Antibacterial/Osteogenic Pattern Switch Properties.	0:159	Jelly-Inspired Injectable Guided Tissue Regeneration Strategy with Shape Auto-Matched and Dual-Light-Defined Antibacterial/Osteogenic Pattern Switch Properties.
33226209	4	36	theme	injectable	626:635	arg1	CTP-SA					673:678	CTP-SA	673:678	CTP-SA	673:678	This study proposed an injectable sodium alginate hydrogel composite (CTP-SA) doped with cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles for GTR.
33226209	4	36	theme	injectable	626:635	arg1	composite					662:670	an injectable sodium alginate hydrogel composite	623:670	an injectable sodium alginate hydrogel composite (CTP-SA) doped with cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles for GTR	623:791	This study proposed an injectable sodium alginate hydrogel composite (CTP-SA) doped with cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles for GTR.
33226209	6	37	theme	light	1022:1026	arg1	irradiation					1033:1043	blue light (BL) irradiation	1017:1043	blue light (BL) irradiation	1017:1043	Meanwhile, CTP-SA exhibited broad-spectrum antibacterial capabilities under blue light (BL) irradiation, including Streptococcus mutans (one of the most abundant bacteria in oral biofilms).
33226209	6	38	theme	Streptococcus	1056:1068	arg1	mutans					1070:1075	Streptococcus mutans	1056:1075	Streptococcus mutans (one of the most abundant bacteria in oral biofilms)	1056:1128	Meanwhile, CTP-SA exhibited broad-spectrum antibacterial capabilities under blue light (BL) irradiation, including Streptococcus mutans (one of the most abundant bacteria in oral biofilms).
33226209	1	39	theme	supporting	244:253	arg1	tissues					255:261	periodontal supporting tissues	232:261	periodontal supporting tissues	232:261	Periodontitis is a bacterial infectious disease leading to the loss of periodontal supporting tissues and teeth.
33226209	0	40	theme	Guided	26:31	arg1	Strategy					53:60	Jelly-Inspired Injectable Guided Tissue Regeneration Strategy	0:60	Jelly-Inspired Injectable Guided Tissue Regeneration Strategy with Shape Auto-Matched and Dual-Light-Defined Antibacterial/Osteogenic Pattern Switch Properties.	0:159	Jelly-Inspired Injectable Guided Tissue Regeneration Strategy with Shape Auto-Matched and Dual-Light-Defined Antibacterial/Osteogenic Pattern Switch Properties.
33226209	4	41	theme	hydrogel	653:660	arg1	CTP-SA					673:678	CTP-SA	673:678	CTP-SA	673:678	This study proposed an injectable sodium alginate hydrogel composite (CTP-SA) doped with cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles for GTR.
33226209	4	41	theme	hydrogel	653:660	arg1	composite					662:670	an injectable sodium alginate hydrogel composite	623:670	an injectable sodium alginate hydrogel composite (CTP-SA) doped with cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles for GTR	623:791	This study proposed an injectable sodium alginate hydrogel composite (CTP-SA) doped with cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles for GTR.
33226209	4	42	theme	@	765:765	arg1	PDA					766:768	TiO2@PDA	761:768	TiO2@PDA	761:768	This study proposed an injectable sodium alginate hydrogel composite (CTP-SA) doped with cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles for GTR.
33226209	4	42	theme	@	765:765	arg1	dioxide					752:758	polydopamine-coated titanium dioxide	723:758	polydopamine-coated titanium dioxide (TiO2@PDA)	723:769	This study proposed an injectable sodium alginate hydrogel composite (CTP-SA) doped with cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles for GTR.
33226209	1	43	theme	tissues	255:261	arg1	loss					224:227	the loss	220:227	the loss of periodontal supporting tissues and teeth	220:271	Periodontitis is a bacterial infectious disease leading to the loss of periodontal supporting tissues and teeth.
33226209	5	44	theme	jelly	834:838	arg1	process					819:825	the gelation process	806:825	the gelation process of the jelly	806:838	Inspired by the gelation process of the jelly, the phase change (liquid to solid) of CTP-SA after injection could automatch variform bone defects.
33226209	4	45	theme	alginate	644:651	arg1	CTP-SA					673:678	CTP-SA	673:678	CTP-SA	673:678	This study proposed an injectable sodium alginate hydrogel composite (CTP-SA) doped with cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles for GTR.
33226209	4	45	theme	alginate	644:651	arg1	composite					662:670	an injectable sodium alginate hydrogel composite	623:670	an injectable sodium alginate hydrogel composite (CTP-SA) doped with cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles for GTR	623:791	This study proposed an injectable sodium alginate hydrogel composite (CTP-SA) doped with cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles for GTR.
33226209	2	46	theme	guided	286:291	arg1	regeneration					300:311	current guided tissue regeneration	278:311	The current guided tissue regeneration (GTR) membranes for periodontitis treatments	274:356	The current guided tissue regeneration (GTR) membranes for periodontitis treatments cannot effectively promote tissue regeneration for the limited antibacterial properties and the excessively fast degradation rate.
33226209	2	46	theme	guided	286:291	arg1	GTR					314:316	GTR	314:316	GTR	314:316	The current guided tissue regeneration (GTR) membranes for periodontitis treatments cannot effectively promote tissue regeneration for the limited antibacterial properties and the excessively fast degradation rate.
33226209	4	47	theme	titanium	743:750	arg1	PDA					766:768	TiO2@PDA	761:768	TiO2@PDA	761:768	This study proposed an injectable sodium alginate hydrogel composite (CTP-SA) doped with cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles for GTR.
33226209	4	47	theme	titanium	743:750	arg1	dioxide					752:758	polydopamine-coated titanium dioxide	723:758	polydopamine-coated titanium dioxide (TiO2@PDA)	723:769	This study proposed an injectable sodium alginate hydrogel composite (CTP-SA) doped with cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles for GTR.
33226209	0	48	theme	Regeneration	40:51	arg1	Strategy					53:60	Jelly-Inspired Injectable Guided Tissue Regeneration Strategy	0:60	Jelly-Inspired Injectable Guided Tissue Regeneration Strategy with Shape Auto-Matched and Dual-Light-Defined Antibacterial/Osteogenic Pattern Switch Properties.	0:159	Jelly-Inspired Injectable Guided Tissue Regeneration Strategy with Shape Auto-Matched and Dual-Light-Defined Antibacterial/Osteogenic Pattern Switch Properties.
33226209	9	49	dep	noninvasive	1490:1500	arg1	NIR					1485:1487	NIR	1485:1487	NIR	1485:1487	Collectively, through this dual-light (blue and NIR) noninvasive regulation, CTP-SA could switch antibacterial and osteogenic modes to address requirements of patients at different healing stages, thereby realizing the customized GTR procedures.
33226209	9	49	dep	noninvasive	1490:1500	arg1	blue					1476:1479	blue	1476:1479	blue	1476:1479	Collectively, through this dual-light (blue and NIR) noninvasive regulation, CTP-SA could switch antibacterial and osteogenic modes to address requirements of patients at different healing stages, thereby realizing the customized GTR procedures.
33226209	2	50	theme	current	278:284	arg1	regeneration					300:311	current guided tissue regeneration	278:311	The current guided tissue regeneration (GTR) membranes for periodontitis treatments	274:356	The current guided tissue regeneration (GTR) membranes for periodontitis treatments cannot effectively promote tissue regeneration for the limited antibacterial properties and the excessively fast degradation rate.
33226209	2	50	theme	current	278:284	arg1	GTR					314:316	GTR	314:316	GTR	314:316	The current guided tissue regeneration (GTR) membranes for periodontitis treatments cannot effectively promote tissue regeneration for the limited antibacterial properties and the excessively fast degradation rate.
33226209	1	51	theme	teeth	267:271	arg1	loss					224:227	the loss	220:227	the loss of periodontal supporting tissues and teeth	220:271	Periodontitis is a bacterial infectious disease leading to the loss of periodontal supporting tissues and teeth.
33226209	5	52	theme	variform	918:925	arg1	defects					932:938	variform bone defects	918:938	variform bone defects	918:938	Inspired by the gelation process of the jelly, the phase change (liquid to solid) of CTP-SA after injection could automatch variform bone defects.
33226209	8	53	theme	effect	1381:1386	arg1	stimulation					1327:1337	the stimulation	1323:1337	the stimulation of newly formed Cu2+ and the photothermal effect of CTP-SA under near-infrared (NIR) irradiation	1323:1434	Afterward, osteogenesis could be enhanced through two factors simultaneously: the stimulation of newly formed Cu2+ and the photothermal effect of CTP-SA under near-infrared (NIR) irradiation.
33226209	3	54	theme	extra	508:512	arg1	tailoring					514:522	extra tailoring	508:522	extra tailoring	508:522	Besides, they need extra tailoring according to variform defects before implantation, leading to imprecise match.
33226209	8	55	theme	formed	1348:1353	arg1	Cu2+					1355:1358	newly formed Cu2+	1342:1358	newly formed Cu2+	1342:1358	Afterward, osteogenesis could be enhanced through two factors simultaneously: the stimulation of newly formed Cu2+ and the photothermal effect of CTP-SA under near-infrared (NIR) irradiation.
33226209	5	56	theme	phase	845:849	arg1	change					851:856	the phase change	841:856	the phase change (liquid to solid) of CTP-SA after injection	841:900	Inspired by the gelation process of the jelly, the phase change (liquid to solid) of CTP-SA after injection could automatch variform bone defects.
33226209	6	57	theme	antibacterial	984:996	arg1	capabilities					998:1009	broad-spectrum antibacterial capabilities	969:1009	broad-spectrum antibacterial capabilities	969:1009	Meanwhile, CTP-SA exhibited broad-spectrum antibacterial capabilities under blue light (BL) irradiation, including Streptococcus mutans (one of the most abundant bacteria in oral biofilms).
33226209	5	58	dep	solid	869:873	arg1	to					866:867	to	866:867	to	866:867	Inspired by the gelation process of the jelly, the phase change (liquid to solid) of CTP-SA after injection could automatch variform bone defects.
33226209	9	59	theme	noninvasive	1490:1500	arg1	regulation					1502:1511	this dual-light (blue and NIR) noninvasive regulation	1459:1511	this dual-light (blue and NIR) noninvasive regulation	1459:1511	Collectively, through this dual-light (blue and NIR) noninvasive regulation, CTP-SA could switch antibacterial and osteogenic modes to address requirements of patients at different healing stages, thereby realizing the customized GTR procedures.
33226209	9	60	theme	different	1608:1616	arg1	stages					1626:1631	different healing stages	1608:1631	different healing stages	1608:1631	Collectively, through this dual-light (blue and NIR) noninvasive regulation, CTP-SA could switch antibacterial and osteogenic modes to address requirements of patients at different healing stages, thereby realizing the customized GTR procedures.
33226209	6	61	theme	broad-spectrum	969:982	arg1	capabilities					998:1009	broad-spectrum antibacterial capabilities	969:1009	broad-spectrum antibacterial capabilities	969:1009	Meanwhile, CTP-SA exhibited broad-spectrum antibacterial capabilities under blue light (BL) irradiation, including Streptococcus mutans (one of the most abundant bacteria in oral biofilms).
33226209	9	62	theme	customized	1656:1665	arg1	procedures					1671:1680	the customized GTR procedures	1652:1680	the customized GTR procedures	1652:1680	Collectively, through this dual-light (blue and NIR) noninvasive regulation, CTP-SA could switch antibacterial and osteogenic modes to address requirements of patients at different healing stages, thereby realizing the customized GTR procedures.
33226209	9	63	theme	osteogenic	1552:1561	arg1	modes					1563:1567	antibacterial and osteogenic modes	1534:1567	antibacterial and osteogenic modes	1534:1567	Collectively, through this dual-light (blue and NIR) noninvasive regulation, CTP-SA could switch antibacterial and osteogenic modes to address requirements of patients at different healing stages, thereby realizing the customized GTR procedures.
33226209	9	64	theme	healing	1618:1624	arg1	stages					1626:1631	different healing stages	1608:1631	different healing stages	1608:1631	Collectively, through this dual-light (blue and NIR) noninvasive regulation, CTP-SA could switch antibacterial and osteogenic modes to address requirements of patients at different healing stages, thereby realizing the customized GTR procedures.
33226209	6	65	theme	bacteria	1103:1110	arg1	bacteria					1103:1110	the most abundant bacteria	1085:1110	the most abundant bacteria in oral biofilms	1085:1127	Meanwhile, CTP-SA exhibited broad-spectrum antibacterial capabilities under blue light (BL) irradiation, including Streptococcus mutans (one of the most abundant bacteria in oral biofilms).
33226209	6	65	theme	bacteria	1103:1110	arg1	one					1078:1080	one	1078:1080	one	1078:1080	Meanwhile, CTP-SA exhibited broad-spectrum antibacterial capabilities under blue light (BL) irradiation, including Streptococcus mutans (one of the most abundant bacteria in oral biofilms).
33226209	1	66	theme	bacterial	180:188	arg1	disease					201:207	a bacterial infectious disease	178:207	a bacterial infectious disease leading to the loss of periodontal supporting tissues and teeth	178:271	Periodontitis is a bacterial infectious disease leading to the loss of periodontal supporting tissues and teeth.
33226209	1	66	theme	bacterial	180:188	arg1	Periodontitis					161:173	Periodontitis	161:173	Periodontitis	161:173	Periodontitis is a bacterial infectious disease leading to the loss of periodontal supporting tissues and teeth.
33226209	8	67	dep	enhanced	1278:1285	arg1	stimulation					1327:1337	the stimulation	1323:1337	the stimulation of newly formed Cu2+ and the photothermal effect of CTP-SA under near-infrared (NIR) irradiation	1323:1434	Afterward, osteogenesis could be enhanced through two factors simultaneously: the stimulation of newly formed Cu2+ and the photothermal effect of CTP-SA under near-infrared (NIR) irradiation.
33226209	5	68	theme	gelation	810:817	arg1	process					819:825	the gelation process	806:825	the gelation process of the jelly	806:838	Inspired by the gelation process of the jelly, the phase change (liquid to solid) of CTP-SA after injection could automatch variform bone defects.
33226209	5	69	theme	CTP-SA	879:884	arg1	change					851:856	the phase change	841:856	the phase change (liquid to solid) of CTP-SA after injection	841:900	Inspired by the gelation process of the jelly, the phase change (liquid to solid) of CTP-SA after injection could automatch variform bone defects.
33226209	2	70	theme	regeneration	300:311	arg1	membranes					319:327	The current guided tissue regeneration (GTR) membranes	274:327	The current guided tissue regeneration (GTR) membranes for periodontitis treatments	274:356	The current guided tissue regeneration (GTR) membranes for periodontitis treatments cannot effectively promote tissue regeneration for the limited antibacterial properties and the excessively fast degradation rate.
33226209	3	71	theme	variform	537:544	arg1	defects					546:552	variform defects	537:552	variform defects before implantation	537:572	Besides, they need extra tailoring according to variform defects before implantation, leading to imprecise match.
33226209	1	72	theme	infectious	190:199	arg1	disease					201:207	a bacterial infectious disease	178:207	a bacterial infectious disease leading to the loss of periodontal supporting tissues and teeth	178:271	Periodontitis is a bacterial infectious disease leading to the loss of periodontal supporting tissues and teeth.
33226209	1	72	theme	infectious	190:199	arg1	Periodontitis					161:173	Periodontitis	161:173	Periodontitis	161:173	Periodontitis is a bacterial infectious disease leading to the loss of periodontal supporting tissues and teeth.
33226209	6	73	theme	oral	1115:1118	arg1	biofilms					1120:1127	oral biofilms	1115:1127	oral biofilms	1115:1127	Meanwhile, CTP-SA exhibited broad-spectrum antibacterial capabilities under blue light (BL) irradiation, including Streptococcus mutans (one of the most abundant bacteria in oral biofilms).
33226209	4	74	theme	cuprous	698:704	arg1	Cu2O					713:716	Cu2O	713:716	Cu2O	713:716	This study proposed an injectable sodium alginate hydrogel composite (CTP-SA) doped with cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles for GTR.
33226209	4	74	theme	cuprous	698:704	arg1	oxide					706:710	cubic cuprous oxide	692:710	cubic cuprous oxide (Cu2O)	692:717	This study proposed an injectable sodium alginate hydrogel composite (CTP-SA) doped with cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles for GTR.
33226209	2	75	theme	tissue	293:298	arg1	regeneration					300:311	current guided tissue regeneration	278:311	The current guided tissue regeneration (GTR) membranes for periodontitis treatments	274:356	The current guided tissue regeneration (GTR) membranes for periodontitis treatments cannot effectively promote tissue regeneration for the limited antibacterial properties and the excessively fast degradation rate.
33226209	2	75	theme	tissue	293:298	arg1	GTR					314:316	GTR	314:316	GTR	314:316	The current guided tissue regeneration (GTR) membranes for periodontitis treatments cannot effectively promote tissue regeneration for the limited antibacterial properties and the excessively fast degradation rate.
33226209	0	76	with	Strategy	53:60	arg1	Auto-Matched					73:84	Auto-Matched	73:84	Auto-Matched	73:84	Jelly-Inspired Injectable Guided Tissue Regeneration Strategy with Shape Auto-Matched and Dual-Light-Defined Antibacterial/Osteogenic Pattern Switch Properties.
33226209	0	76	with	Strategy	53:60	arg1	Properties					149:158	Shape Auto-Matched and Dual-Light-Defined Antibacterial/Osteogenic Pattern Switch Properties	67:158	Properties	149:158	Jelly-Inspired Injectable Guided Tissue Regeneration Strategy with Shape Auto-Matched and Dual-Light-Defined Antibacterial/Osteogenic Pattern Switch Properties.
33226209	5	77	theme	bone	927:930	arg1	defects					932:938	variform bone defects	918:938	variform bone defects	918:938	Inspired by the gelation process of the jelly, the phase change (liquid to solid) of CTP-SA after injection could automatch variform bone defects.
33226209	4	78	theme	cubic	692:696	arg1	Cu2O					713:716	Cu2O	713:716	Cu2O	713:716	This study proposed an injectable sodium alginate hydrogel composite (CTP-SA) doped with cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles for GTR.
33226209	4	78	theme	cubic	692:696	arg1	oxide					706:710	cubic cuprous oxide	692:710	cubic cuprous oxide (Cu2O)	692:717	This study proposed an injectable sodium alginate hydrogel composite (CTP-SA) doped with cubic cuprous oxide (Cu2O) and polydopamine-coated titanium dioxide (TiO2@PDA) nanoparticles for GTR.
33517204	11	0	theme	optimal	1330:1336	arg1	conditions					1338:1347	the optimal conditions	1326:1347	the optimal conditions	1326:1347	Under the optimal conditions, the electrochemical impedance spectroscopy (EIS) was proportional to the PSA biomarker concentration from 0.1 pg mL-1 to 100 ng mL-1 with low limit of detection (0.078 pg mL-1).
33517204	11	1	theme	detection	1501:1509	arg1	limit					1492:1496	low limit	1488:1496	low limit of detection (0.078 pg mL-1)	1488:1525	Under the optimal conditions, the electrochemical impedance spectroscopy (EIS) was proportional to the PSA biomarker concentration from 0.1 pg mL-1 to 100 ng mL-1 with low limit of detection (0.078 pg mL-1).
33517204	2	2	theme	cancer	313:318	arg1	types1					320:325	more than 200 different cancer types1	289:325	more than 200 different cancer types1	289:325	In males, prostate cancer is ranked second as leading cause of death out of more than 200 different cancer types1.
33517204	6	3	theme	label	660:664	arg1	immunosensor					682:693	a free label electrochemical immunosensor	653:693	a free label electrochemical immunosensor	653:693	In this study, a free label electrochemical immunosensor was prepared based on a new green platform for the quantitative detection of the PSA.
33517204	5	4	theme	cancer	618:623	arg1	progression					625:635	cancer progression	618:635	cancer progression	618:635	Therefore, PSA determination is important to cancer progression.
33517204	9	5	theme	antibody	1132:1139	arg1	loading					1141:1147	the antibody loading	1128:1147	the antibody loading	1128:1147	This nanocomposite was used to increase the antibody loading and accelerate the electron transfer, which can increase the biosensor sensitivity.
33517204	2	6	theme	leading	259:265	arg1	cancer					232:237	prostate cancer	223:237	prostate cancer	223:237	In males, prostate cancer is ranked second as leading cause of death out of more than 200 different cancer types1.
33517204	2	6	theme	leading	259:265	arg1	cause					267:271	leading cause	259:271	leading cause of death out of more than 200 different cancer types1	259:325	In males, prostate cancer is ranked second as leading cause of death out of more than 200 different cancer types1.
33517204	11	7	theme	electrochemical	1354:1368	arg1	EIS					1394:1396	EIS	1394:1396	EIS	1394:1396	Under the optimal conditions, the electrochemical impedance spectroscopy (EIS) was proportional to the PSA biomarker concentration from 0.1 pg mL-1 to 100 ng mL-1 with low limit of detection (0.078 pg mL-1).
33517204	11	7	theme	electrochemical	1354:1368	arg1	proportional					1403:1414	proportional	1403:1414	proportional	1403:1414	Under the optimal conditions, the electrochemical impedance spectroscopy (EIS) was proportional to the PSA biomarker concentration from 0.1 pg mL-1 to 100 ng mL-1 with low limit of detection (0.078 pg mL-1).
33517204	11	7	theme	electrochemical	1354:1368	arg1	spectroscopy					1380:1391	the electrochemical impedance spectroscopy	1350:1391	the electrochemical impedance spectroscopy (EIS)	1350:1397	Under the optimal conditions, the electrochemical impedance spectroscopy (EIS) was proportional to the PSA biomarker concentration from 0.1 pg mL-1 to 100 ng mL-1 with low limit of detection (0.078 pg mL-1).
33517204	7	8	theme	used	785:788	arg1	platform					790:797	The used platform	781:797	The used platform	781:797	The used platform was formed from quince seed mucilage containing green gold and silver nanoparticles and synthesized by the green method (using Calendula officinalis L. extract).
33517204	13	9	theme	sample	1714:1719	arg1	analysis					1721:1728	real sample analysis	1709:1728	real sample analysis	1709:1728	The results of real sample analysis presented another tool for the PSA biomarker detection in physiologic models.
33517204	6	10	theme	green	723:727	arg1	platform					729:736	a new green platform	717:736	a new green platform for the quantitative detection of the PSA	717:778	In this study, a free label electrochemical immunosensor was prepared based on a new green platform for the quantitative detection of the PSA.
33517204	12	11	theme	high	1570:1573	arg1	stability					1575:1583	high stability	1570:1583	high stability	1570:1583	The proposed green immunosensor exhibited high stability and reproducibility, which can be used for the quantitative assay of the PSA biomarker in clinical analyses.
33517204	12	12	from	analyses	1684:1691	arg1	assay					1645:1649	the quantitative assay	1628:1649	the quantitative assay of the PSA biomarker in clinical analyses	1628:1691	The proposed green immunosensor exhibited high stability and reproducibility, which can be used for the quantitative assay of the PSA biomarker in clinical analyses.
33517204	1	13	theme	cancer	205:210	arg1	antigen					155:161	Prostatic specific antigen	136:161	Prostatic specific antigen (PSA)	136:167	Prostatic specific antigen (PSA) is known as a biomarker of prostate cancer.
33517204	1	13	theme	cancer	205:210	arg1	biomarker					183:191	a biomarker	181:191	a biomarker of prostate cancer	181:210	Prostatic specific antigen (PSA) is known as a biomarker of prostate cancer.
33517204	12	14	theme	proposed	1532:1539	arg1	immunosensor					1547:1558	The proposed green immunosensor	1528:1558	The proposed green immunosensor	1528:1558	The proposed green immunosensor exhibited high stability and reproducibility, which can be used for the quantitative assay of the PSA biomarker in clinical analyses.
33517204	7	15	theme	L.	948:949	arg1	extract					951:957	Calendula officinalis L. extract	926:957	Calendula officinalis L. extract	926:957	The used platform was formed from quince seed mucilage containing green gold and silver nanoparticles and synthesized by the green method (using Calendula officinalis L. extract).
33517204	9	16	theme	biosensor	1210:1218	arg1	sensitivity					1220:1230	the biosensor sensitivity	1206:1230	the biosensor sensitivity	1206:1230	This nanocomposite was used to increase the antibody loading and accelerate the electron transfer, which can increase the biosensor sensitivity.
33517204	7	17	theme	Calendula	926:934	arg1	extract					951:957	Calendula officinalis L. extract	926:957	Calendula officinalis L. extract	926:957	The used platform was formed from quince seed mucilage containing green gold and silver nanoparticles and synthesized by the green method (using Calendula officinalis L. extract).
33517204	10	18	theme	biomarker	1259:1267	arg1	antibodies					1237:1246	The antibodies	1233:1246	The antibodies of the PSA biomarker	1233:1267	The antibodies of the PSA biomarker were successfully incubated on the green platform.
33517204	0	19	theme	antigen	69:75	arg1	detection					37:45	detection	37:45	detection of prostatic specific antigen using quince seed mucilage-GNPs-SNPs as a green composite	37:133	The electrochemical immunosensor for detection of prostatic specific antigen using quince seed mucilage-GNPs-SNPs as a green composite.
33517204	2	20	theme	prostate	223:230	arg1	cancer					232:237	prostate cancer	223:237	prostate cancer	223:237	In males, prostate cancer is ranked second as leading cause of death out of more than 200 different cancer types1.
33517204	2	20	theme	prostate	223:230	arg1	cause					267:271	leading cause	259:271	leading cause of death out of more than 200 different cancer types1	259:325	In males, prostate cancer is ranked second as leading cause of death out of more than 200 different cancer types1.
33517204	11	21	with	100 ng mL-1	1471:1481	arg1	limit					1492:1496	low limit	1488:1496	low limit of detection (0.078 pg mL-1)	1488:1525	Under the optimal conditions, the electrochemical impedance spectroscopy (EIS) was proportional to the PSA biomarker concentration from 0.1 pg mL-1 to 100 ng mL-1 with low limit of detection (0.078 pg mL-1).
33517204	11	22	theme	low	1488:1490	arg1	limit					1492:1496	low limit	1488:1496	low limit of detection (0.078 pg mL-1)	1488:1525	Under the optimal conditions, the electrochemical impedance spectroscopy (EIS) was proportional to the PSA biomarker concentration from 0.1 pg mL-1 to 100 ng mL-1 with low limit of detection (0.078 pg mL-1).
33517204	0	23	theme	quince	83:88	arg1	composite					125:133	a green composite	117:133	a green composite	117:133	The electrochemical immunosensor for detection of prostatic specific antigen using quince seed mucilage-GNPs-SNPs as a green composite.
33517204	0	23	theme	quince	83:88	arg1	mucilage-GNPs-SNPs					95:112	quince seed mucilage-GNPs-SNPs	83:112	quince seed mucilage-GNPs-SNPs	83:112	The electrochemical immunosensor for detection of prostatic specific antigen using quince seed mucilage-GNPs-SNPs as a green composite.
33517204	7	24	theme	green	906:910	arg1	method					912:917	the green method	902:917	the green method (using Calendula officinalis L. extract)	902:958	The used platform was formed from quince seed mucilage containing green gold and silver nanoparticles and synthesized by the green method (using Calendula officinalis L. extract).
33517204	12	25	theme	PSA	1658:1660	arg1	biomarker					1662:1670	the PSA biomarker	1654:1670	the PSA biomarker in clinical analyses	1654:1691	The proposed green immunosensor exhibited high stability and reproducibility, which can be used for the quantitative assay of the PSA biomarker in clinical analyses.
33517204	6	26	theme	quantitative	746:757	arg1	detection					759:767	the quantitative detection	742:767	the quantitative detection of the PSA	742:778	In this study, a free label electrochemical immunosensor was prepared based on a new green platform for the quantitative detection of the PSA.
33517204	2	27	dep	types1	320:325	arg1	out					282:284	out	282:284	out	282:284	In males, prostate cancer is ranked second as leading cause of death out of more than 200 different cancer types1.
33517204	8	28	theme	mucilage	972:979	arg1	biopolymer					981:990	The quince mucilage biopolymer	961:990	The quince mucilage biopolymer	961:990	The quince mucilage biopolymer was used as a sub layer to assemble nanoparticles and increase the electrochemical performance.
33517204	8	28	theme	mucilage	972:979	arg1	layer					1010:1014	a sub layer	1004:1014	a sub layer	1004:1014	The quince mucilage biopolymer was used as a sub layer to assemble nanoparticles and increase the electrochemical performance.
33517204	12	29	used	used	1619:1622	arg2	reproducibility					1589:1603	reproducibility	1589:1603	reproducibility	1589:1603	The proposed green immunosensor exhibited high stability and reproducibility, which can be used for the quantitative assay of the PSA biomarker in clinical analyses.
33517204	12	29	used	used	1619:1622	arg2	stability					1575:1583	high stability	1570:1583	high stability	1570:1583	The proposed green immunosensor exhibited high stability and reproducibility, which can be used for the quantitative assay of the PSA biomarker in clinical analyses.
33517204	1	30	theme	specific	146:153	arg1	PSA					164:166	PSA	164:166	PSA	164:166	Prostatic specific antigen (PSA) is known as a biomarker of prostate cancer.
33517204	1	30	theme	specific	146:153	arg1	biomarker					183:191	a biomarker	181:191	a biomarker of prostate cancer	181:210	Prostatic specific antigen (PSA) is known as a biomarker of prostate cancer.
33517204	1	30	theme	specific	146:153	arg1	antigen					155:161	Prostatic specific antigen	136:161	Prostatic specific antigen (PSA)	136:167	Prostatic specific antigen (PSA) is known as a biomarker of prostate cancer.
33517204	3	31	theme	significant	379:389	arg1	reduction					391:399	a significant reduction	377:399	a significant reduction in mortality	377:412	As a result, early detection of cancer can cause a significant reduction in mortality.
33517204	3	31	theme	significant	379:389	arg1	result					333:338	a result	331:338	a result	331:338	As a result, early detection of cancer can cause a significant reduction in mortality.
33517204	12	32	theme	quantitative	1632:1643	arg1	assay					1645:1649	the quantitative assay	1628:1649	the quantitative assay of the PSA biomarker in clinical analyses	1628:1691	The proposed green immunosensor exhibited high stability and reproducibility, which can be used for the quantitative assay of the PSA biomarker in clinical analyses.
33517204	7	33	theme	silver	862:867	arg1	nanoparticles					869:881	green gold and silver nanoparticles	847:881	green gold and silver nanoparticles	847:881	The used platform was formed from quince seed mucilage containing green gold and silver nanoparticles and synthesized by the green method (using Calendula officinalis L. extract).
33517204	11	34	theme	PSA	1423:1425	arg1	concentration					1437:1449	the PSA biomarker concentration	1419:1449	the PSA biomarker concentration from 0.1 pg mL-1 to 100 ng mL-1 with low limit of detection (0.078 pg mL-1)	1419:1525	Under the optimal conditions, the electrochemical impedance spectroscopy (EIS) was proportional to the PSA biomarker concentration from 0.1 pg mL-1 to 100 ng mL-1 with low limit of detection (0.078 pg mL-1).
33517204	8	35	theme	sub	1006:1008	arg1	biopolymer					981:990	The quince mucilage biopolymer	961:990	The quince mucilage biopolymer	961:990	The quince mucilage biopolymer was used as a sub layer to assemble nanoparticles and increase the electrochemical performance.
33517204	8	35	theme	sub	1006:1008	arg1	layer					1010:1014	a sub layer	1004:1014	a sub layer	1004:1014	The quince mucilage biopolymer was used as a sub layer to assemble nanoparticles and increase the electrochemical performance.
33517204	11	36	from	0.1 pg mL-1	1456:1466	arg1	concentration					1437:1449	the PSA biomarker concentration	1419:1449	the PSA biomarker concentration from 0.1 pg mL-1 to 100 ng mL-1 with low limit of detection (0.078 pg mL-1)	1419:1525	Under the optimal conditions, the electrochemical impedance spectroscopy (EIS) was proportional to the PSA biomarker concentration from 0.1 pg mL-1 to 100 ng mL-1 with low limit of detection (0.078 pg mL-1).
33517204	3	37	theme	cancer	360:365	arg1	detection					347:355	early detection	341:355	early detection of cancer	341:365	As a result, early detection of cancer can cause a significant reduction in mortality.
33517204	2	38	theme	different	303:311	arg1	types1					320:325	more than 200 different cancer types1	289:325	more than 200 different cancer types1	289:325	In males, prostate cancer is ranked second as leading cause of death out of more than 200 different cancer types1.
33517204	0	39	theme	prostatic	50:58	arg1	antigen					69:75	prostatic specific antigen	50:75	prostatic specific antigen using quince seed mucilage-GNPs-SNPs as a green composite	50:133	The electrochemical immunosensor for detection of prostatic specific antigen using quince seed mucilage-GNPs-SNPs as a green composite.
33517204	4	40	theme	serum	483:487	arg1	concentrations					489:502	normal serum concentrations	476:502	normal serum concentrations in healthy means	476:519	PSA concentration directly is related to prostate cancer, so normal serum concentrations in healthy means are 4 ng and above 10 ng as abnormal concentration.
33517204	6	41	theme	PSA	776:778	arg1	detection					759:767	the quantitative detection	742:767	the quantitative detection of the PSA	742:778	In this study, a free label electrochemical immunosensor was prepared based on a new green platform for the quantitative detection of the PSA.
33517204	13	42	theme	PSA	1761:1763	arg1	detection					1775:1783	the PSA biomarker detection	1757:1783	the PSA biomarker detection	1757:1783	The results of real sample analysis presented another tool for the PSA biomarker detection in physiologic models.
33517204	8	43	theme	electrochemical	1059:1073	arg1	performance					1075:1085	the electrochemical performance	1055:1085	the electrochemical performance	1055:1085	The quince mucilage biopolymer was used as a sub layer to assemble nanoparticles and increase the electrochemical performance.
33517204	3	44	from	reduction	391:399	arg1	mortality					404:412	mortality	404:412	mortality	404:412	As a result, early detection of cancer can cause a significant reduction in mortality.
33517204	2	45	theme	types1	320:325	arg1	cancer					232:237	prostate cancer	223:237	prostate cancer	223:237	In males, prostate cancer is ranked second as leading cause of death out of more than 200 different cancer types1.
33517204	2	45	theme	types1	320:325	arg1	cause					267:271	leading cause	259:271	leading cause of death out of more than 200 different cancer types1	259:325	In males, prostate cancer is ranked second as leading cause of death out of more than 200 different cancer types1.
33517204	11	46	theme	impedance	1370:1378	arg1	EIS					1394:1396	EIS	1394:1396	EIS	1394:1396	Under the optimal conditions, the electrochemical impedance spectroscopy (EIS) was proportional to the PSA biomarker concentration from 0.1 pg mL-1 to 100 ng mL-1 with low limit of detection (0.078 pg mL-1).
33517204	11	46	theme	impedance	1370:1378	arg1	proportional					1403:1414	proportional	1403:1414	proportional	1403:1414	Under the optimal conditions, the electrochemical impedance spectroscopy (EIS) was proportional to the PSA biomarker concentration from 0.1 pg mL-1 to 100 ng mL-1 with low limit of detection (0.078 pg mL-1).
33517204	11	46	theme	impedance	1370:1378	arg1	spectroscopy					1380:1391	the electrochemical impedance spectroscopy	1350:1391	the electrochemical impedance spectroscopy (EIS)	1350:1397	Under the optimal conditions, the electrochemical impedance spectroscopy (EIS) was proportional to the PSA biomarker concentration from 0.1 pg mL-1 to 100 ng mL-1 with low limit of detection (0.078 pg mL-1).
33517204	7	47	theme	quince	815:820	arg1	mucilage					827:834	quince seed mucilage	815:834	quince seed mucilage containing green gold and silver nanoparticles	815:881	The used platform was formed from quince seed mucilage containing green gold and silver nanoparticles and synthesized by the green method (using Calendula officinalis L. extract).
33517204	13	48	theme	real	1709:1712	arg1	analysis					1721:1728	real sample analysis	1709:1728	real sample analysis	1709:1728	The results of real sample analysis presented another tool for the PSA biomarker detection in physiologic models.
33517204	5	49	theme	PSA	584:586	arg1	determination					588:600	PSA determination	584:600	PSA determination	584:600	Therefore, PSA determination is important to cancer progression.
33517204	13	50	theme	analysis	1721:1728	arg1	results					1698:1704	The results	1694:1704	The results of real sample analysis	1694:1728	The results of real sample analysis presented another tool for the PSA biomarker detection in physiologic models.
33517204	6	51	theme	new	719:721	arg1	platform					729:736	a new green platform	717:736	a new green platform for the quantitative detection of the PSA	717:778	In this study, a free label electrochemical immunosensor was prepared based on a new green platform for the quantitative detection of the PSA.
33517204	2	52	theme	death	276:280	arg1	cancer					232:237	prostate cancer	223:237	prostate cancer	223:237	In males, prostate cancer is ranked second as leading cause of death out of more than 200 different cancer types1.
33517204	2	52	theme	death	276:280	arg1	cause					267:271	leading cause	259:271	leading cause of death out of more than 200 different cancer types1	259:325	In males, prostate cancer is ranked second as leading cause of death out of more than 200 different cancer types1.
33517204	1	53	theme	prostate	196:203	arg1	cancer					205:210	prostate cancer	196:210	prostate cancer	196:210	Prostatic specific antigen (PSA) is known as a biomarker of prostate cancer.
33517204	12	54	theme	green	1541:1545	arg1	immunosensor					1547:1558	The proposed green immunosensor	1528:1558	The proposed green immunosensor	1528:1558	The proposed green immunosensor exhibited high stability and reproducibility, which can be used for the quantitative assay of the PSA biomarker in clinical analyses.
33517204	4	55	theme	PSA	415:417	arg1	concentration					419:431	PSA concentration	415:431	PSA concentration	415:431	PSA concentration directly is related to prostate cancer, so normal serum concentrations in healthy means are 4 ng and above 10 ng as abnormal concentration.
33517204	13	56	from	tool	1748:1751	arg1	models					1800:1805	physiologic models	1788:1805	physiologic models	1788:1805	The results of real sample analysis presented another tool for the PSA biomarker detection in physiologic models.
33517204	12	57	from	biomarker	1662:1670	arg1	analyses					1684:1691	clinical analyses	1675:1691	clinical analyses	1675:1691	The proposed green immunosensor exhibited high stability and reproducibility, which can be used for the quantitative assay of the PSA biomarker in clinical analyses.
33517204	9	58	theme	electron	1168:1175	arg1	transfer					1177:1184	the electron transfer	1164:1184	the electron transfer	1164:1184	This nanocomposite was used to increase the antibody loading and accelerate the electron transfer, which can increase the biosensor sensitivity.
33517204	1	59	theme	Prostatic	136:144	arg1	PSA					164:166	PSA	164:166	PSA	164:166	Prostatic specific antigen (PSA) is known as a biomarker of prostate cancer.
33517204	1	59	theme	Prostatic	136:144	arg1	biomarker					183:191	a biomarker	181:191	a biomarker of prostate cancer	181:210	Prostatic specific antigen (PSA) is known as a biomarker of prostate cancer.
33517204	1	59	theme	Prostatic	136:144	arg1	antigen					155:161	Prostatic specific antigen	136:161	Prostatic specific antigen (PSA)	136:167	Prostatic specific antigen (PSA) is known as a biomarker of prostate cancer.
33517204	12	60	theme	biomarker	1662:1670	arg1	assay					1645:1649	the quantitative assay	1628:1649	the quantitative assay of the PSA biomarker in clinical analyses	1628:1691	The proposed green immunosensor exhibited high stability and reproducibility, which can be used for the quantitative assay of the PSA biomarker in clinical analyses.
33517204	11	61	theme	biomarker	1427:1435	arg1	concentration					1437:1449	the PSA biomarker concentration	1419:1449	the PSA biomarker concentration from 0.1 pg mL-1 to 100 ng mL-1 with low limit of detection (0.078 pg mL-1)	1419:1525	Under the optimal conditions, the electrochemical impedance spectroscopy (EIS) was proportional to the PSA biomarker concentration from 0.1 pg mL-1 to 100 ng mL-1 with low limit of detection (0.078 pg mL-1).
33517204	7	62	theme	officinalis	936:946	arg1	extract					951:957	Calendula officinalis L. extract	926:957	Calendula officinalis L. extract	926:957	The used platform was formed from quince seed mucilage containing green gold and silver nanoparticles and synthesized by the green method (using Calendula officinalis L. extract).
33517204	12	63	theme	clinical	1675:1682	arg1	analyses					1684:1691	clinical analyses	1675:1691	clinical analyses	1675:1691	The proposed green immunosensor exhibited high stability and reproducibility, which can be used for the quantitative assay of the PSA biomarker in clinical analyses.
33517204	4	64	theme	prostate	456:463	arg1	cancer					465:470	prostate cancer	456:470	prostate cancer	456:470	PSA concentration directly is related to prostate cancer, so normal serum concentrations in healthy means are 4 ng and above 10 ng as abnormal concentration.
33517204	12	65	from	assay	1645:1649	arg1	analyses					1684:1691	clinical analyses	1675:1691	clinical analyses	1675:1691	The proposed green immunosensor exhibited high stability and reproducibility, which can be used for the quantitative assay of the PSA biomarker in clinical analyses.
33517204	0	66	theme	seed	90:93	arg1	composite					125:133	a green composite	117:133	a green composite	117:133	The electrochemical immunosensor for detection of prostatic specific antigen using quince seed mucilage-GNPs-SNPs as a green composite.
33517204	0	66	theme	seed	90:93	arg1	mucilage-GNPs-SNPs					95:112	quince seed mucilage-GNPs-SNPs	83:112	quince seed mucilage-GNPs-SNPs	83:112	The electrochemical immunosensor for detection of prostatic specific antigen using quince seed mucilage-GNPs-SNPs as a green composite.
33517204	8	67	theme	quince	965:970	arg1	biopolymer					981:990	The quince mucilage biopolymer	961:990	The quince mucilage biopolymer	961:990	The quince mucilage biopolymer was used as a sub layer to assemble nanoparticles and increase the electrochemical performance.
33517204	8	67	theme	quince	965:970	arg1	layer					1010:1014	a sub layer	1004:1014	a sub layer	1004:1014	The quince mucilage biopolymer was used as a sub layer to assemble nanoparticles and increase the electrochemical performance.
33517204	6	68	theme	free	655:658	arg1	immunosensor					682:693	a free label electrochemical immunosensor	653:693	a free label electrochemical immunosensor	653:693	In this study, a free label electrochemical immunosensor was prepared based on a new green platform for the quantitative detection of the PSA.
33517204	0	69	theme	green	119:123	arg1	composite					125:133	a green composite	117:133	a green composite	117:133	The electrochemical immunosensor for detection of prostatic specific antigen using quince seed mucilage-GNPs-SNPs as a green composite.
33517204	0	69	theme	green	119:123	arg1	mucilage-GNPs-SNPs					95:112	quince seed mucilage-GNPs-SNPs	83:112	quince seed mucilage-GNPs-SNPs	83:112	The electrochemical immunosensor for detection of prostatic specific antigen using quince seed mucilage-GNPs-SNPs as a green composite.
33517204	8	70	used	used	996:999	arg2	biopolymer					981:990	The quince mucilage biopolymer	961:990	The quince mucilage biopolymer	961:990	The quince mucilage biopolymer was used as a sub layer to assemble nanoparticles and increase the electrochemical performance.
33517204	8	70	used	used	996:999	arg2	layer					1010:1014	a sub layer	1004:1014	a sub layer	1004:1014	The quince mucilage biopolymer was used as a sub layer to assemble nanoparticles and increase the electrochemical performance.
33517204	7	71	contain	containing	836:845	arg1	mucilage					827:834	quince seed mucilage	815:834	quince seed mucilage containing green gold and silver nanoparticles	815:881	The used platform was formed from quince seed mucilage containing green gold and silver nanoparticles and synthesized by the green method (using Calendula officinalis L. extract).
33517204	7	71	contain	containing	836:845	arg2	nanoparticles					869:881	green gold and silver nanoparticles	847:881	green gold and silver nanoparticles	847:881	The used platform was formed from quince seed mucilage containing green gold and silver nanoparticles and synthesized by the green method (using Calendula officinalis L. extract).
33517204	0	72	theme	electrochemical	4:18	arg1	immunosensor					20:31	The electrochemical immunosensor	0:31	The electrochemical immunosensor for detection of prostatic specific antigen using quince seed mucilage-GNPs-SNPs as a green composite.	0:134	The electrochemical immunosensor for detection of prostatic specific antigen using quince seed mucilage-GNPs-SNPs as a green composite.
33517204	3	73	theme	early	341:345	arg1	detection					347:355	early detection	341:355	early detection of cancer	341:365	As a result, early detection of cancer can cause a significant reduction in mortality.
33517204	4	74	theme	healthy	507:513	arg1	means					515:519	healthy means	507:519	healthy means	507:519	PSA concentration directly is related to prostate cancer, so normal serum concentrations in healthy means are 4 ng and above 10 ng as abnormal concentration.
33517204	13	75	theme	physiologic	1788:1798	arg1	models					1800:1805	physiologic models	1788:1805	physiologic models	1788:1805	The results of real sample analysis presented another tool for the PSA biomarker detection in physiologic models.
33517204	10	76	theme	green	1304:1308	arg1	platform					1310:1317	the green platform	1300:1317	the green platform	1300:1317	The antibodies of the PSA biomarker were successfully incubated on the green platform.
33517204	10	77	theme	PSA	1255:1257	arg1	biomarker					1259:1267	the PSA biomarker	1251:1267	the PSA biomarker	1251:1267	The antibodies of the PSA biomarker were successfully incubated on the green platform.
33517204	4	78	from	concentrations	489:502	arg1	means					515:519	healthy means	507:519	healthy means	507:519	PSA concentration directly is related to prostate cancer, so normal serum concentrations in healthy means are 4 ng and above 10 ng as abnormal concentration.
33517204	4	79	theme	normal	476:481	arg1	concentrations					489:502	normal serum concentrations	476:502	normal serum concentrations in healthy means	476:519	PSA concentration directly is related to prostate cancer, so normal serum concentrations in healthy means are 4 ng and above 10 ng as abnormal concentration.
33517204	9	80	used	used	1111:1114	arg2	nanocomposite					1093:1105	This nanocomposite	1088:1105	This nanocomposite	1088:1105	This nanocomposite was used to increase the antibody loading and accelerate the electron transfer, which can increase the biosensor sensitivity.
33517204	0	81	theme	specific	60:67	arg1	antigen					69:75	prostatic specific antigen	50:75	prostatic specific antigen using quince seed mucilage-GNPs-SNPs as a green composite	50:133	The electrochemical immunosensor for detection of prostatic specific antigen using quince seed mucilage-GNPs-SNPs as a green composite.
33517204	7	82	theme	gold	853:856	arg1	nanoparticles					869:881	green gold and silver nanoparticles	847:881	green gold and silver nanoparticles	847:881	The used platform was formed from quince seed mucilage containing green gold and silver nanoparticles and synthesized by the green method (using Calendula officinalis L. extract).
33517204	4	83	theme	abnormal	549:556	arg1	concentration					558:570	abnormal concentration	549:570	abnormal concentration	549:570	PSA concentration directly is related to prostate cancer, so normal serum concentrations in healthy means are 4 ng and above 10 ng as abnormal concentration.
33517204	13	84	theme	biomarker	1765:1773	arg1	detection					1775:1783	the PSA biomarker detection	1757:1783	the PSA biomarker detection	1757:1783	The results of real sample analysis presented another tool for the PSA biomarker detection in physiologic models.
33517204	6	85	theme	electrochemical	666:680	arg1	immunosensor					682:693	a free label electrochemical immunosensor	653:693	a free label electrochemical immunosensor	653:693	In this study, a free label electrochemical immunosensor was prepared based on a new green platform for the quantitative detection of the PSA.
33517204	7	86	theme	seed	822:825	arg1	mucilage					827:834	quince seed mucilage	815:834	quince seed mucilage containing green gold and silver nanoparticles	815:881	The used platform was formed from quince seed mucilage containing green gold and silver nanoparticles and synthesized by the green method (using Calendula officinalis L. extract).
31985393	14	0	theme	type	1699:1702	arg1	T11bT					1714:1718	T11bT	1714:1718	T11bT (=CIP 111621T=CCM 8854T=LMG 30632T=DSM 107127T)	1714:1766	The type strain is T11bT (=CIP 111621T=CCM 8854T=LMG 30632T=DSM 107127T).
31985393	14	0	theme	type	1699:1702	arg1	strain					1704:1709	The type strain	1695:1709	The type strain	1695:1709	The type strain is T11bT (=CIP 111621T=CCM 8854T=LMG 30632T=DSM 107127T).
31985393	11	1	theme	related	1507:1513	arg1	species					1515:1521	the next related species	1498:1521	the next related species	1498:1521	Moderate to low DNA-DNA similarities, physiological traits as well as unique traits in the fatty acid pattern distinguished strain T11bT from the next related species.
31985393	3	2	theme	siccitolerans	502:514	arg1	strains					473:479	the type strains	464:479	the type strains of Pseudarthrobacter siccitolerans (98.1 %) and Arthrobacter methylotrophus and Pseudarthrobacter phenanthrenivorans (both 98.0 %)	464:610	A comparative analysis of the 16S rRNA gene sequence showed highest similarities to the type strains of Pseudarthrobacter siccitolerans (98.1 %) and Arthrobacter methylotrophus and Pseudarthrobacter phenanthrenivorans (both 98.0 %).
31985393	10	3	theme	fatty	1237:1241	arg1	profile					1248:1254	the fatty acid profile	1233:1254	the fatty acid profile	1233:1254	In the fatty acid profile, anteiso and iso branched fatty acids predominated (anteiso C15 : 0, iso C16 : 0, anteiso C17 : 0).
31985393	1	4	theme	Salmo	90:94	arg1	salmon					82:87	a farmed Atlantic salmon	64:87	a farmed Atlantic salmon (Salmo salar)	64:101	nov., isolated from an ulcer of a farmed Atlantic salmon (Salmo salar), and emended description of the genus Arthrobacter sensu lato.
31985393	1	4	theme	Salmo	90:94	arg1	salar					96:100	Salmo salar	90:100	Salmo salar	90:100	nov., isolated from an ulcer of a farmed Atlantic salmon (Salmo salar), and emended description of the genus Arthrobacter sensu lato.
31985393	4	5	theme	highest	617:623	arg1	%					785:785	81.15 and 80.99 %	769:785	81.15 and 80.99 %	769:785	The highest ANI value observed between the assembled genome of T11bT and the publicly available Pseudarthrobacter and Arthrobacter type strain genomes were 81.15 and 80.99 %, respectively.
31985393	4	5	theme	highest	617:623	arg1	value					629:633	The highest ANI value	613:633	The highest ANI value observed between the assembled genome of T11bT and the publicly available Pseudarthrobacter and Arthrobacter type strain genomes	613:762	The highest ANI value observed between the assembled genome of T11bT and the publicly available Pseudarthrobacter and Arthrobacter type strain genomes were 81.15 and 80.99 %, respectively.
31985393	1	6	theme	genus	135:139	arg1	lato					160:163	the genus Arthrobacter sensu lato	131:163	the genus Arthrobacter sensu lato	131:163	nov., isolated from an ulcer of a farmed Atlantic salmon (Salmo salar), and emended description of the genus Arthrobacter sensu lato.
31985393	2	7	theme	Atlantic	266:273	arg1	salar					289:293	Salmo salar	283:293	Salmo salar	283:293	A Gram-stain positive, pleomorphic, oxidase-negative, non-motile isolate from the ulcer of a farmed Atlantic salmon (Salmo salar), designated strain T11bT, was subjected to a comprehensive taxonomic investigation.
31985393	2	7	theme	Atlantic	266:273	arg1	salmon					275:280	a farmed Atlantic salmon	257:280	a farmed Atlantic salmon (Salmo salar)	257:294	A Gram-stain positive, pleomorphic, oxidase-negative, non-motile isolate from the ulcer of a farmed Atlantic salmon (Salmo salar), designated strain T11bT, was subjected to a comprehensive taxonomic investigation.
31985393	1	8	theme	sensu	154:158	arg1	lato					160:163	the genus Arthrobacter sensu lato	131:163	the genus Arthrobacter sensu lato	131:163	nov., isolated from an ulcer of a farmed Atlantic salmon (Salmo salar), and emended description of the genus Arthrobacter sensu lato.
31985393	1	9	theme	Atlantic	73:80	arg1	salmon					82:87	a farmed Atlantic salmon	64:87	a farmed Atlantic salmon (Salmo salar)	64:101	nov., isolated from an ulcer of a farmed Atlantic salmon (Salmo salar), and emended description of the genus Arthrobacter sensu lato.
31985393	1	9	theme	Atlantic	73:80	arg1	salar					96:100	Salmo salar	90:100	Salmo salar	90:100	nov., isolated from an ulcer of a farmed Atlantic salmon (Salmo salar), and emended description of the genus Arthrobacter sensu lato.
31985393	5	10	theme	respiratory	812:822	arg1	quinone					824:830	The major respiratory quinone	802:830	The major respiratory quinone	802:830	The major respiratory quinone was menaquinone MK-9(H2).
31985393	5	10	theme	respiratory	812:822	arg1	MK-9					848:851	menaquinone MK-9	836:851	menaquinone MK-9(H2)	836:855	The major respiratory quinone was menaquinone MK-9(H2).
31985393	2	11	theme	positive	179:186	arg1	isolate					231:237	A Gram-stain positive, pleomorphic, oxidase-negative, non-motile isolate	166:237	A Gram-stain positive, pleomorphic, oxidase-negative, non-motile isolate	166:237	A Gram-stain positive, pleomorphic, oxidase-negative, non-motile isolate from the ulcer of a farmed Atlantic salmon (Salmo salar), designated strain T11bT, was subjected to a comprehensive taxonomic investigation.
31985393	11	12	theme	strain	1480:1485	arg1	T11bT					1487:1491	strain T11bT	1480:1491	strain T11bT	1480:1491	Moderate to low DNA-DNA similarities, physiological traits as well as unique traits in the fatty acid pattern distinguished strain T11bT from the next related species.
31985393	10	13	dep	C15 	1316:1319	arg1	anteiso					1338:1344	anteiso	1338:1344	anteiso	1338:1344	In the fatty acid profile, anteiso and iso branched fatty acids predominated (anteiso C15 : 0, iso C16 : 0, anteiso C17 : 0).
31985393	10	13	dep	C15 	1316:1319	arg1	 0					1351:1352	 0	1351:1352	 0	1351:1352	In the fatty acid profile, anteiso and iso branched fatty acids predominated (anteiso C15 : 0, iso C16 : 0, anteiso C17 : 0).
31985393	10	13	dep	C15 	1316:1319	arg1	 0					1321:1322	 0	1321:1322	 0	1321:1322	In the fatty acid profile, anteiso and iso branched fatty acids predominated (anteiso C15 : 0, iso C16 : 0, anteiso C17 : 0).
31985393	10	13	dep	C15 	1316:1319	arg1	C17 					1346:1349	C17 	1346:1349	C17 	1346:1349	In the fatty acid profile, anteiso and iso branched fatty acids predominated (anteiso C15 : 0, iso C16 : 0, anteiso C17 : 0).
31985393	10	14	theme	anteiso	1257:1263	arg1	acids					1288:1292	anteiso and iso branched fatty acids	1257:1292	anteiso and iso branched fatty acids	1257:1292	In the fatty acid profile, anteiso and iso branched fatty acids predominated (anteiso C15 : 0, iso C16 : 0, anteiso C17 : 0).
31985393	3	15	theme	phenanthrenivorans	579:596	arg1	strains					473:479	the type strains	464:479	the type strains of Pseudarthrobacter siccitolerans (98.1 %) and Arthrobacter methylotrophus and Pseudarthrobacter phenanthrenivorans (both 98.0 %)	464:610	A comparative analysis of the 16S rRNA gene sequence showed highest similarities to the type strains of Pseudarthrobacter siccitolerans (98.1 %) and Arthrobacter methylotrophus and Pseudarthrobacter phenanthrenivorans (both 98.0 %).
31985393	4	16	theme	assembled	656:664	arg1	genome					666:671	the assembled genome	652:671	the assembled genome of T11bT and the publicly available Pseudarthrobacter and Arthrobacter type strain genomes	652:762	The highest ANI value observed between the assembled genome of T11bT and the publicly available Pseudarthrobacter and Arthrobacter type strain genomes were 81.15 and 80.99 %, respectively.
31985393	11	17	theme	next	1502:1505	arg1	species					1515:1521	the next related species	1498:1521	the next related species	1498:1521	Moderate to low DNA-DNA similarities, physiological traits as well as unique traits in the fatty acid pattern distinguished strain T11bT from the next related species.
31985393	12	18	theme	Arthrobacter	1658:1669	arg1	sp					1686:1687	the name Arthrobacter ulcerisalmonis sp	1649:1687	the name Arthrobacter ulcerisalmonis sp	1649:1687	All these data point to the fact that strain T11bT represents a novel species of the genus Arthrobacter for which we propose the name Arthrobacter ulcerisalmonis sp.
31985393	10	19	theme	fatty	1282:1286	arg1	acids					1288:1292	anteiso and iso branched fatty acids	1257:1292	anteiso and iso branched fatty acids	1257:1292	In the fatty acid profile, anteiso and iso branched fatty acids predominated (anteiso C15 : 0, iso C16 : 0, anteiso C17 : 0).
31985393	7	20	theme	major	957:961	arg1	dimannosylglyceride					1050:1068	dimannosylglyceride	1050:1068	dimannosylglyceride	1050:1068	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol, monogalactosyl-diacylglycerol and dimannosylglyceride.
31985393	7	20	theme	major	957:961	arg1	monogalactosyl-diacylglycerol					1016:1044	monogalactosyl-diacylglycerol	1016:1044	monogalactosyl-diacylglycerol	1016:1044	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol, monogalactosyl-diacylglycerol and dimannosylglyceride.
31985393	7	20	theme	major	957:961	arg1	lipids					963:968	the major lipids diphosphatidylglycerol, phosphatidylglycerol, monogalactosyl-diacylglycerol and dimannosylglyceride	953:1068	the major lipids diphosphatidylglycerol, phosphatidylglycerol, monogalactosyl-diacylglycerol and dimannosylglyceride	953:1068	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol, monogalactosyl-diacylglycerol and dimannosylglyceride.
31985393	7	20	theme	major	957:961	arg1	phosphatidylglycerol					994:1013	phosphatidylglycerol	994:1013	phosphatidylglycerol	994:1013	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol, monogalactosyl-diacylglycerol and dimannosylglyceride.
31985393	7	20	theme	major	957:961	arg1	diphosphatidylglycerol					970:991	diphosphatidylglycerol	970:991	diphosphatidylglycerol	970:991	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol, monogalactosyl-diacylglycerol and dimannosylglyceride.
31985393	7	21	theme	polar	920:924	arg1	profile					932:938	The polar lipid profile	916:938	The polar lipid profile	916:938	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol, monogalactosyl-diacylglycerol and dimannosylglyceride.
31985393	2	22	theme	taxonomic	355:363	arg1	investigation					365:377	a comprehensive taxonomic investigation	339:377	a comprehensive taxonomic investigation	339:377	A Gram-stain positive, pleomorphic, oxidase-negative, non-motile isolate from the ulcer of a farmed Atlantic salmon (Salmo salar), designated strain T11bT, was subjected to a comprehensive taxonomic investigation.
31985393	4	23	theme	strain	749:754	arg1	genomes					756:762	type strain genomes	744:762	type strain genomes	744:762	The highest ANI value observed between the assembled genome of T11bT and the publicly available Pseudarthrobacter and Arthrobacter type strain genomes were 81.15 and 80.99 %, respectively.
31985393	11	24	theme	unique	1426:1431	arg1	traits					1433:1438	unique traits	1426:1438	physiological traits as well as unique traits in the fatty acid pattern	1394:1464	Moderate to low DNA-DNA similarities, physiological traits as well as unique traits in the fatty acid pattern distinguished strain T11bT from the next related species.
31985393	3	25	theme	comparative	382:392	arg1	analysis					394:401	A comparative analysis	380:401	A comparative analysis of the 16S rRNA gene sequence	380:431	A comparative analysis of the 16S rRNA gene sequence showed highest similarities to the type strains of Pseudarthrobacter siccitolerans (98.1 %) and Arthrobacter methylotrophus and Pseudarthrobacter phenanthrenivorans (both 98.0 %).
31985393	8	26	theme	Minor	1071:1075	arg1	amouts					1077:1082	Minor amouts	1071:1082	Minor amouts of trimannosyldiacylglycerol and phosphatidylinositol	1071:1136	Minor amouts of trimannosyldiacylglycerol and phosphatidylinositol were also detected.
31985393	0	27	theme	Arthrobacter	0:11	arg1	sp					28:29	Arthrobacter ulcerisalmonis sp	0:29	Arthrobacter ulcerisalmonis sp.	0:30	Arthrobacter ulcerisalmonis sp.
31985393	4	28	dep	T11bT	676:680	arg1	genomes					756:762	type strain genomes	744:762	type strain genomes	744:762	The highest ANI value observed between the assembled genome of T11bT and the publicly available Pseudarthrobacter and Arthrobacter type strain genomes were 81.15 and 80.99 %, respectively.
31985393	4	29	theme	Pseudarthrobacter	709:725	arg1	genome					666:671	the assembled genome	652:671	the assembled genome of T11bT and the publicly available Pseudarthrobacter and Arthrobacter type strain genomes	652:762	The highest ANI value observed between the assembled genome of T11bT and the publicly available Pseudarthrobacter and Arthrobacter type strain genomes were 81.15 and 80.99 %, respectively.
31985393	11	30	theme	fatty	1447:1451	arg1	pattern					1458:1464	the fatty acid pattern	1443:1464	the fatty acid pattern	1443:1464	Moderate to low DNA-DNA similarities, physiological traits as well as unique traits in the fatty acid pattern distinguished strain T11bT from the next related species.
31985393	3	31	theme	16S	410:412	arg1	rRNA					414:417	the 16S rRNA	406:417	the 16S rRNA gene sequence	406:431	A comparative analysis of the 16S rRNA gene sequence showed highest similarities to the type strains of Pseudarthrobacter siccitolerans (98.1 %) and Arthrobacter methylotrophus and Pseudarthrobacter phenanthrenivorans (both 98.0 %).
31985393	12	32	theme	genus	1609:1613	arg1	Arthrobacter					1615:1626	the genus Arthrobacter	1605:1626	the genus Arthrobacter	1605:1626	All these data point to the fact that strain T11bT represents a novel species of the genus Arthrobacter for which we propose the name Arthrobacter ulcerisalmonis sp.
31985393	7	33	dep	lipids	963:968	arg1	dimannosylglyceride					1050:1068	dimannosylglyceride	1050:1068	dimannosylglyceride	1050:1068	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol, monogalactosyl-diacylglycerol and dimannosylglyceride.
31985393	7	33	dep	lipids	963:968	arg1	monogalactosyl-diacylglycerol					1016:1044	monogalactosyl-diacylglycerol	1016:1044	monogalactosyl-diacylglycerol	1016:1044	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol, monogalactosyl-diacylglycerol and dimannosylglyceride.
31985393	7	33	dep	lipids	963:968	arg1	lipids					963:968	the major lipids diphosphatidylglycerol, phosphatidylglycerol, monogalactosyl-diacylglycerol and dimannosylglyceride	953:1068	the major lipids diphosphatidylglycerol, phosphatidylglycerol, monogalactosyl-diacylglycerol and dimannosylglyceride	953:1068	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol, monogalactosyl-diacylglycerol and dimannosylglyceride.
31985393	7	33	dep	lipids	963:968	arg1	phosphatidylglycerol					994:1013	phosphatidylglycerol	994:1013	phosphatidylglycerol	994:1013	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol, monogalactosyl-diacylglycerol and dimannosylglyceride.
31985393	7	33	dep	lipids	963:968	arg1	diphosphatidylglycerol					970:991	diphosphatidylglycerol	970:991	diphosphatidylglycerol	970:991	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol, monogalactosyl-diacylglycerol and dimannosylglyceride.
31985393	2	34	theme	comprehensive	341:353	arg1	investigation					365:377	a comprehensive taxonomic investigation	339:377	a comprehensive taxonomic investigation	339:377	A Gram-stain positive, pleomorphic, oxidase-negative, non-motile isolate from the ulcer of a farmed Atlantic salmon (Salmo salar), designated strain T11bT, was subjected to a comprehensive taxonomic investigation.
31985393	14	35	theme	8854T=LMG	1738:1746	arg1	T11bT					1714:1718	T11bT	1714:1718	T11bT (=CIP 111621T=CCM 8854T=LMG 30632T=DSM 107127T)	1714:1766	The type strain is T11bT (=CIP 111621T=CCM 8854T=LMG 30632T=DSM 107127T).
31985393	14	35	theme	8854T=LMG	1738:1746	arg1	107127T					1759:1765	=CIP 111621T=CCM 8854T=LMG 30632T=DSM 107127T	1721:1765	=CIP 111621T=CCM 8854T=LMG 30632T=DSM 107127T	1721:1765	The type strain is T11bT (=CIP 111621T=CCM 8854T=LMG 30632T=DSM 107127T).
31985393	10	36	theme	iso	1269:1271	arg1	acids					1288:1292	anteiso and iso branched fatty acids	1257:1292	anteiso and iso branched fatty acids	1257:1292	In the fatty acid profile, anteiso and iso branched fatty acids predominated (anteiso C15 : 0, iso C16 : 0, anteiso C17 : 0).
31985393	2	37	theme	Salmo	283:287	arg1	salar					289:293	Salmo salar	283:293	Salmo salar	283:293	A Gram-stain positive, pleomorphic, oxidase-negative, non-motile isolate from the ulcer of a farmed Atlantic salmon (Salmo salar), designated strain T11bT, was subjected to a comprehensive taxonomic investigation.
31985393	2	37	theme	Salmo	283:287	arg1	salmon					275:280	a farmed Atlantic salmon	257:280	a farmed Atlantic salmon (Salmo salar)	257:294	A Gram-stain positive, pleomorphic, oxidase-negative, non-motile isolate from the ulcer of a farmed Atlantic salmon (Salmo salar), designated strain T11bT, was subjected to a comprehensive taxonomic investigation.
31985393	14	38	theme	=CIP	1721:1724	arg1	T11bT					1714:1718	T11bT	1714:1718	T11bT (=CIP 111621T=CCM 8854T=LMG 30632T=DSM 107127T)	1714:1766	The type strain is T11bT (=CIP 111621T=CCM 8854T=LMG 30632T=DSM 107127T).
31985393	14	38	theme	=CIP	1721:1724	arg1	107127T					1759:1765	=CIP 111621T=CCM 8854T=LMG 30632T=DSM 107127T	1721:1765	=CIP 111621T=CCM 8854T=LMG 30632T=DSM 107127T	1721:1765	The type strain is T11bT (=CIP 111621T=CCM 8854T=LMG 30632T=DSM 107127T).
31985393	11	39	theme	low	1368:1370	arg1	similarities					1380:1391	low DNA-DNA similarities	1368:1391	low DNA-DNA similarities	1368:1391	Moderate to low DNA-DNA similarities, physiological traits as well as unique traits in the fatty acid pattern distinguished strain T11bT from the next related species.
31985393	6	40	theme	polyamine	862:870	arg1	pattern					872:878	The polyamine pattern	858:878	The polyamine pattern	858:878	The polyamine pattern contained predominantly spermidine.
31985393	11	41	theme	physiological	1394:1406	arg1	traits					1408:1413	physiological traits	1394:1413	physiological traits as well as unique traits in the fatty acid pattern	1394:1464	Moderate to low DNA-DNA similarities, physiological traits as well as unique traits in the fatty acid pattern distinguished strain T11bT from the next related species.
31985393	3	42	theme	Pseudarthrobacter	484:500	arg1	siccitolerans					502:514	Pseudarthrobacter siccitolerans	484:514	Pseudarthrobacter siccitolerans (98.1 %)	484:523	A comparative analysis of the 16S rRNA gene sequence showed highest similarities to the type strains of Pseudarthrobacter siccitolerans (98.1 %) and Arthrobacter methylotrophus and Pseudarthrobacter phenanthrenivorans (both 98.0 %).
31985393	3	42	theme	Pseudarthrobacter	484:500	arg1	%					522:522	98.1 %	517:522	98.1 %	517:522	A comparative analysis of the 16S rRNA gene sequence showed highest similarities to the type strains of Pseudarthrobacter siccitolerans (98.1 %) and Arthrobacter methylotrophus and Pseudarthrobacter phenanthrenivorans (both 98.0 %).
31985393	10	43	theme	acid	1243:1246	arg1	profile					1248:1254	the fatty acid profile	1233:1254	the fatty acid profile	1233:1254	In the fatty acid profile, anteiso and iso branched fatty acids predominated (anteiso C15 : 0, iso C16 : 0, anteiso C17 : 0).
31985393	1	44	theme	Arthrobacter	141:152	arg1	lato					160:163	the genus Arthrobacter sensu lato	131:163	the genus Arthrobacter sensu lato	131:163	nov., isolated from an ulcer of a farmed Atlantic salmon (Salmo salar), and emended description of the genus Arthrobacter sensu lato.
31985393	2	45	theme	salmon	275:280	arg1	ulcer					248:252	the ulcer	244:252	the ulcer of a farmed Atlantic salmon (Salmo salar)	244:294	A Gram-stain positive, pleomorphic, oxidase-negative, non-motile isolate from the ulcer of a farmed Atlantic salmon (Salmo salar), designated strain T11bT, was subjected to a comprehensive taxonomic investigation.
31985393	5	46	theme	menaquinone	836:846	arg1	H2					853:854	H2	853:854	H2	853:854	The major respiratory quinone was menaquinone MK-9(H2).
31985393	5	46	theme	menaquinone	836:846	arg1	quinone					824:830	The major respiratory quinone	802:830	The major respiratory quinone	802:830	The major respiratory quinone was menaquinone MK-9(H2).
31985393	5	46	theme	menaquinone	836:846	arg1	MK-9					848:851	menaquinone MK-9	836:851	menaquinone MK-9(H2)	836:855	The major respiratory quinone was menaquinone MK-9(H2).
31985393	6	47	contain	contained	880:888	arg1	pattern					872:878	The polyamine pattern	858:878	The polyamine pattern	858:878	The polyamine pattern contained predominantly spermidine.
31985393	6	47	contain	contained	880:888	arg2	spermidine					904:913	spermidine	904:913	spermidine	904:913	The polyamine pattern contained predominantly spermidine.
31985393	2	48	theme	farmed	259:264	arg1	salar					289:293	Salmo salar	283:293	Salmo salar	283:293	A Gram-stain positive, pleomorphic, oxidase-negative, non-motile isolate from the ulcer of a farmed Atlantic salmon (Salmo salar), designated strain T11bT, was subjected to a comprehensive taxonomic investigation.
31985393	2	48	theme	farmed	259:264	arg1	salmon					275:280	a farmed Atlantic salmon	257:280	a farmed Atlantic salmon (Salmo salar)	257:294	A Gram-stain positive, pleomorphic, oxidase-negative, non-motile isolate from the ulcer of a farmed Atlantic salmon (Salmo salar), designated strain T11bT, was subjected to a comprehensive taxonomic investigation.
31985393	1	49	theme	lato	160:163	arg1	description					116:126	description	116:126	description of the genus Arthrobacter sensu lato	116:163	nov., isolated from an ulcer of a farmed Atlantic salmon (Salmo salar), and emended description of the genus Arthrobacter sensu lato.
31985393	12	50	theme	novel	1588:1592	arg1	species					1594:1600	a novel species	1586:1600	a novel species of the genus Arthrobacter for which we propose the name Arthrobacter ulcerisalmonis sp	1586:1687	All these data point to the fact that strain T11bT represents a novel species of the genus Arthrobacter for which we propose the name Arthrobacter ulcerisalmonis sp.
31985393	2	51	dep	positive	179:186	arg1	pleomorphic					189:199	pleomorphic	189:199	pleomorphic	189:199	A Gram-stain positive, pleomorphic, oxidase-negative, non-motile isolate from the ulcer of a farmed Atlantic salmon (Salmo salar), designated strain T11bT, was subjected to a comprehensive taxonomic investigation.
31985393	2	51	dep	positive	179:186	arg1	non-motile					220:229	non-motile	220:229	non-motile	220:229	A Gram-stain positive, pleomorphic, oxidase-negative, non-motile isolate from the ulcer of a farmed Atlantic salmon (Salmo salar), designated strain T11bT, was subjected to a comprehensive taxonomic investigation.
31985393	2	51	dep	positive	179:186	arg1	oxidase-negative					202:217	oxidase-negative	202:217	oxidase-negative	202:217	A Gram-stain positive, pleomorphic, oxidase-negative, non-motile isolate from the ulcer of a farmed Atlantic salmon (Salmo salar), designated strain T11bT, was subjected to a comprehensive taxonomic investigation.
31985393	3	52	theme	methylotrophus	542:555	arg1	phenanthrenivorans					579:596	Arthrobacter methylotrophus and Pseudarthrobacter phenanthrenivorans	529:596	Arthrobacter methylotrophus and Pseudarthrobacter phenanthrenivorans (both 98.0 %)	529:610	A comparative analysis of the 16S rRNA gene sequence showed highest similarities to the type strains of Pseudarthrobacter siccitolerans (98.1 %) and Arthrobacter methylotrophus and Pseudarthrobacter phenanthrenivorans (both 98.0 %).
31985393	3	52	theme	methylotrophus	542:555	arg1	%					609:609	98.0 %	604:609	98.0 %	604:609	A comparative analysis of the 16S rRNA gene sequence showed highest similarities to the type strains of Pseudarthrobacter siccitolerans (98.1 %) and Arthrobacter methylotrophus and Pseudarthrobacter phenanthrenivorans (both 98.0 %).
31985393	4	53	theme	ANI	625:627	arg1	%					785:785	81.15 and 80.99 %	769:785	81.15 and 80.99 %	769:785	The highest ANI value observed between the assembled genome of T11bT and the publicly available Pseudarthrobacter and Arthrobacter type strain genomes were 81.15 and 80.99 %, respectively.
31985393	4	53	theme	ANI	625:627	arg1	value					629:633	The highest ANI value	613:633	The highest ANI value observed between the assembled genome of T11bT and the publicly available Pseudarthrobacter and Arthrobacter type strain genomes	613:762	The highest ANI value observed between the assembled genome of T11bT and the publicly available Pseudarthrobacter and Arthrobacter type strain genomes were 81.15 and 80.99 %, respectively.
31985393	5	54	theme	major	806:810	arg1	quinone					824:830	The major respiratory quinone	802:830	The major respiratory quinone	802:830	The major respiratory quinone was menaquinone MK-9(H2).
31985393	5	54	theme	major	806:810	arg1	MK-9					848:851	menaquinone MK-9	836:851	menaquinone MK-9(H2)	836:855	The major respiratory quinone was menaquinone MK-9(H2).
31985393	11	55	from	traits	1408:1413	arg1	pattern					1458:1464	the fatty acid pattern	1443:1464	the fatty acid pattern	1443:1464	Moderate to low DNA-DNA similarities, physiological traits as well as unique traits in the fatty acid pattern distinguished strain T11bT from the next related species.
31985393	4	56	dep	Pseudarthrobacter	709:725	arg1	the					686:688	the	686:688	the	686:688	The highest ANI value observed between the assembled genome of T11bT and the publicly available Pseudarthrobacter and Arthrobacter type strain genomes were 81.15 and 80.99 %, respectively.
31985393	12	57	theme	strain	1562:1567	arg1	T11bT					1569:1573	strain T11bT	1562:1573	strain T11bT	1562:1573	All these data point to the fact that strain T11bT represents a novel species of the genus Arthrobacter for which we propose the name Arthrobacter ulcerisalmonis sp.
31985393	10	58	theme	branched	1273:1280	arg1	acids					1288:1292	anteiso and iso branched fatty acids	1257:1292	anteiso and iso branched fatty acids	1257:1292	In the fatty acid profile, anteiso and iso branched fatty acids predominated (anteiso C15 : 0, iso C16 : 0, anteiso C17 : 0).
31985393	12	59	theme	ulcerisalmonis	1671:1684	arg1	sp					1686:1687	the name Arthrobacter ulcerisalmonis sp	1649:1687	the name Arthrobacter ulcerisalmonis sp	1649:1687	All these data point to the fact that strain T11bT represents a novel species of the genus Arthrobacter for which we propose the name Arthrobacter ulcerisalmonis sp.
31985393	9	60	theme	type	1187:1190	arg1	l-Lys-l-Ser-l-Thr-l-Ala					1196:1218	the type A3α l-Lys-l-Ser-l-Thr-l-Ala	1183:1218	the type A3α l-Lys-l-Ser-l-Thr-l-Ala (A11.23)	1183:1227	The peptidoglycan was of the type A3α l-Lys-l-Ser-l-Thr-l-Ala (A11.23).
31985393	9	60	theme	type	1187:1190	arg1	A11.23					1221:1226	A11.23	1221:1226	A11.23	1221:1226	The peptidoglycan was of the type A3α l-Lys-l-Ser-l-Thr-l-Ala (A11.23).
31985393	4	61	theme	T11bT	676:680	arg1	genome					666:671	the assembled genome	652:671	the assembled genome of T11bT and the publicly available Pseudarthrobacter and Arthrobacter type strain genomes	652:762	The highest ANI value observed between the assembled genome of T11bT and the publicly available Pseudarthrobacter and Arthrobacter type strain genomes were 81.15 and 80.99 %, respectively.
31985393	11	62	theme	acid	1453:1456	arg1	pattern					1458:1464	the fatty acid pattern	1443:1464	the fatty acid pattern	1443:1464	Moderate to low DNA-DNA similarities, physiological traits as well as unique traits in the fatty acid pattern distinguished strain T11bT from the next related species.
31985393	3	63	theme	gene	419:422	arg1	sequence					424:431	the 16S rRNA gene sequence	406:431	the 16S rRNA gene sequence	406:431	A comparative analysis of the 16S rRNA gene sequence showed highest similarities to the type strains of Pseudarthrobacter siccitolerans (98.1 %) and Arthrobacter methylotrophus and Pseudarthrobacter phenanthrenivorans (both 98.0 %).
31985393	3	64	theme	Pseudarthrobacter	561:577	arg1	phenanthrenivorans					579:596	Arthrobacter methylotrophus and Pseudarthrobacter phenanthrenivorans	529:596	Arthrobacter methylotrophus and Pseudarthrobacter phenanthrenivorans (both 98.0 %)	529:610	A comparative analysis of the 16S rRNA gene sequence showed highest similarities to the type strains of Pseudarthrobacter siccitolerans (98.1 %) and Arthrobacter methylotrophus and Pseudarthrobacter phenanthrenivorans (both 98.0 %).
31985393	3	64	theme	Pseudarthrobacter	561:577	arg1	%					609:609	98.0 %	604:609	98.0 %	604:609	A comparative analysis of the 16S rRNA gene sequence showed highest similarities to the type strains of Pseudarthrobacter siccitolerans (98.1 %) and Arthrobacter methylotrophus and Pseudarthrobacter phenanthrenivorans (both 98.0 %).
31985393	2	65	from	ulcer	248:252	arg1	isolate					231:237	A Gram-stain positive, pleomorphic, oxidase-negative, non-motile isolate	166:237	A Gram-stain positive, pleomorphic, oxidase-negative, non-motile isolate	166:237	A Gram-stain positive, pleomorphic, oxidase-negative, non-motile isolate from the ulcer of a farmed Atlantic salmon (Salmo salar), designated strain T11bT, was subjected to a comprehensive taxonomic investigation.
31985393	12	66	theme	name	1653:1656	arg1	sp					1686:1687	the name Arthrobacter ulcerisalmonis sp	1649:1687	the name Arthrobacter ulcerisalmonis sp	1649:1687	All these data point to the fact that strain T11bT represents a novel species of the genus Arthrobacter for which we propose the name Arthrobacter ulcerisalmonis sp.
31985393	1	67	attach	isolated	38:45	arg2	nov.					32:35	nov.	32:35	nov.	32:35	nov., isolated from an ulcer of a farmed Atlantic salmon (Salmo salar), and emended description of the genus Arthrobacter sensu lato.
31985393	1	67	attach	isolated	38:45	arg1	ulcer					55:59	an ulcer	52:59	an ulcer of a farmed Atlantic salmon (Salmo salar)	52:101	nov., isolated from an ulcer of a farmed Atlantic salmon (Salmo salar), and emended description of the genus Arthrobacter sensu lato.
31985393	10	68	dep	predominated	1294:1305	arg1	C16 					1329:1332	iso C16 	1325:1332	iso C16 	1325:1332	In the fatty acid profile, anteiso and iso branched fatty acids predominated (anteiso C15 : 0, iso C16 : 0, anteiso C17 : 0).
31985393	10	68	dep	predominated	1294:1305	arg1	C15 					1316:1319	C15 	1316:1319	C15 	1316:1319	In the fatty acid profile, anteiso and iso branched fatty acids predominated (anteiso C15 : 0, iso C16 : 0, anteiso C17 : 0).
31985393	12	69	theme	Arthrobacter	1615:1626	arg1	species					1594:1600	a novel species	1586:1600	a novel species of the genus Arthrobacter for which we propose the name Arthrobacter ulcerisalmonis sp	1586:1687	All these data point to the fact that strain T11bT represents a novel species of the genus Arthrobacter for which we propose the name Arthrobacter ulcerisalmonis sp.
31985393	4	70	theme	type	744:747	arg1	genomes					756:762	type strain genomes	744:762	type strain genomes	744:762	The highest ANI value observed between the assembled genome of T11bT and the publicly available Pseudarthrobacter and Arthrobacter type strain genomes were 81.15 and 80.99 %, respectively.
31985393	0	71	theme	ulcerisalmonis	13:26	arg1	sp					28:29	Arthrobacter ulcerisalmonis sp	0:29	Arthrobacter ulcerisalmonis sp.	0:30	Arthrobacter ulcerisalmonis sp.
31985393	8	72	theme	trimannosyldiacylglycerol	1087:1111	arg1	amouts					1077:1082	Minor amouts	1071:1082	Minor amouts of trimannosyldiacylglycerol and phosphatidylinositol	1071:1136	Minor amouts of trimannosyldiacylglycerol and phosphatidylinositol were also detected.
31985393	1	73	theme	farmed	66:71	arg1	salmon					82:87	a farmed Atlantic salmon	64:87	a farmed Atlantic salmon (Salmo salar)	64:101	nov., isolated from an ulcer of a farmed Atlantic salmon (Salmo salar), and emended description of the genus Arthrobacter sensu lato.
31985393	1	73	theme	farmed	66:71	arg1	salar					96:100	Salmo salar	90:100	Salmo salar	90:100	nov., isolated from an ulcer of a farmed Atlantic salmon (Salmo salar), and emended description of the genus Arthrobacter sensu lato.
31985393	8	74	theme	phosphatidylinositol	1117:1136	arg1	amouts					1077:1082	Minor amouts	1071:1082	Minor amouts of trimannosyldiacylglycerol and phosphatidylinositol	1071:1136	Minor amouts of trimannosyldiacylglycerol and phosphatidylinositol were also detected.
31985393	7	75	theme	lipid	926:930	arg1	profile					932:938	The polar lipid profile	916:938	The polar lipid profile	916:938	The polar lipid profile consisted of the major lipids diphosphatidylglycerol, phosphatidylglycerol, monogalactosyl-diacylglycerol and dimannosylglyceride.
31985393	1	76	theme	salmon	82:87	arg1	ulcer					55:59	an ulcer	52:59	an ulcer of a farmed Atlantic salmon (Salmo salar)	52:101	nov., isolated from an ulcer of a farmed Atlantic salmon (Salmo salar), and emended description of the genus Arthrobacter sensu lato.
31985393	10	77	theme	iso	1325:1327	arg1	C16 					1329:1332	iso C16 	1325:1332	iso C16 	1325:1332	In the fatty acid profile, anteiso and iso branched fatty acids predominated (anteiso C15 : 0, iso C16 : 0, anteiso C17 : 0).
31985393	10	77	theme	iso	1325:1327	arg1	C15 					1316:1319	C15 	1316:1319	C15 	1316:1319	In the fatty acid profile, anteiso and iso branched fatty acids predominated (anteiso C15 : 0, iso C16 : 0, anteiso C17 : 0).
31985393	3	78	theme	rRNA	414:417	arg1	sequence					424:431	the 16S rRNA gene sequence	406:431	the 16S rRNA gene sequence	406:431	A comparative analysis of the 16S rRNA gene sequence showed highest similarities to the type strains of Pseudarthrobacter siccitolerans (98.1 %) and Arthrobacter methylotrophus and Pseudarthrobacter phenanthrenivorans (both 98.0 %).
31985393	11	79	from	traits	1433:1438	arg1	pattern					1458:1464	the fatty acid pattern	1443:1464	the fatty acid pattern	1443:1464	Moderate to low DNA-DNA similarities, physiological traits as well as unique traits in the fatty acid pattern distinguished strain T11bT from the next related species.
31985393	14	80	theme	30632T=DSM	1748:1757	arg1	T11bT					1714:1718	T11bT	1714:1718	T11bT (=CIP 111621T=CCM 8854T=LMG 30632T=DSM 107127T)	1714:1766	The type strain is T11bT (=CIP 111621T=CCM 8854T=LMG 30632T=DSM 107127T).
31985393	14	80	theme	30632T=DSM	1748:1757	arg1	107127T					1759:1765	=CIP 111621T=CCM 8854T=LMG 30632T=DSM 107127T	1721:1765	=CIP 111621T=CCM 8854T=LMG 30632T=DSM 107127T	1721:1765	The type strain is T11bT (=CIP 111621T=CCM 8854T=LMG 30632T=DSM 107127T).
31985393	2	81	theme	strain	308:313	arg1	T11bT					315:319	strain T11bT	308:319	strain T11bT	308:319	A Gram-stain positive, pleomorphic, oxidase-negative, non-motile isolate from the ulcer of a farmed Atlantic salmon (Salmo salar), designated strain T11bT, was subjected to a comprehensive taxonomic investigation.
31985393	2	82	theme	Gram-stain	168:177	arg1	isolate					231:237	A Gram-stain positive, pleomorphic, oxidase-negative, non-motile isolate	166:237	A Gram-stain positive, pleomorphic, oxidase-negative, non-motile isolate	166:237	A Gram-stain positive, pleomorphic, oxidase-negative, non-motile isolate from the ulcer of a farmed Atlantic salmon (Salmo salar), designated strain T11bT, was subjected to a comprehensive taxonomic investigation.
31985393	3	83	theme	highest	440:446	arg1	similarities					448:459	highest similarities	440:459	highest similarities to the type strains of Pseudarthrobacter siccitolerans (98.1 %) and Arthrobacter methylotrophus and Pseudarthrobacter phenanthrenivorans (both 98.0 %)	440:610	A comparative analysis of the 16S rRNA gene sequence showed highest similarities to the type strains of Pseudarthrobacter siccitolerans (98.1 %) and Arthrobacter methylotrophus and Pseudarthrobacter phenanthrenivorans (both 98.0 %).
31985393	14	84	theme	111621T=CCM	1726:1736	arg1	T11bT					1714:1718	T11bT	1714:1718	T11bT (=CIP 111621T=CCM 8854T=LMG 30632T=DSM 107127T)	1714:1766	The type strain is T11bT (=CIP 111621T=CCM 8854T=LMG 30632T=DSM 107127T).
31985393	14	84	theme	111621T=CCM	1726:1736	arg1	107127T					1759:1765	=CIP 111621T=CCM 8854T=LMG 30632T=DSM 107127T	1721:1765	=CIP 111621T=CCM 8854T=LMG 30632T=DSM 107127T	1721:1765	The type strain is T11bT (=CIP 111621T=CCM 8854T=LMG 30632T=DSM 107127T).
31985393	9	85	theme	A3α	1192:1194	arg1	l-Lys-l-Ser-l-Thr-l-Ala					1196:1218	the type A3α l-Lys-l-Ser-l-Thr-l-Ala	1183:1218	the type A3α l-Lys-l-Ser-l-Thr-l-Ala (A11.23)	1183:1227	The peptidoglycan was of the type A3α l-Lys-l-Ser-l-Thr-l-Ala (A11.23).
31985393	9	85	theme	A3α	1192:1194	arg1	A11.23					1221:1226	A11.23	1221:1226	A11.23	1221:1226	The peptidoglycan was of the type A3α l-Lys-l-Ser-l-Thr-l-Ala (A11.23).
31985393	3	86	theme	sequence	424:431	arg1	analysis					394:401	A comparative analysis	380:401	A comparative analysis of the 16S rRNA gene sequence	380:431	A comparative analysis of the 16S rRNA gene sequence showed highest similarities to the type strains of Pseudarthrobacter siccitolerans (98.1 %) and Arthrobacter methylotrophus and Pseudarthrobacter phenanthrenivorans (both 98.0 %).
31985393	3	87	dep	%	609:609	arg1	both					599:602	both	599:602	both	599:602	A comparative analysis of the 16S rRNA gene sequence showed highest similarities to the type strains of Pseudarthrobacter siccitolerans (98.1 %) and Arthrobacter methylotrophus and Pseudarthrobacter phenanthrenivorans (both 98.0 %).
31985393	11	88	theme	DNA-DNA	1372:1378	arg1	similarities					1380:1391	low DNA-DNA similarities	1368:1391	low DNA-DNA similarities	1368:1391	Moderate to low DNA-DNA similarities, physiological traits as well as unique traits in the fatty acid pattern distinguished strain T11bT from the next related species.
31985393	3	89	theme	type	468:471	arg1	strains					473:479	the type strains	464:479	the type strains of Pseudarthrobacter siccitolerans (98.1 %) and Arthrobacter methylotrophus and Pseudarthrobacter phenanthrenivorans (both 98.0 %)	464:610	A comparative analysis of the 16S rRNA gene sequence showed highest similarities to the type strains of Pseudarthrobacter siccitolerans (98.1 %) and Arthrobacter methylotrophus and Pseudarthrobacter phenanthrenivorans (both 98.0 %).
31943028	11	0	theme	methionine	1736:1745	arg1	concentrations					1747:1760	plasma methionine concentrations	1729:1760	plasma methionine concentrations	1729:1760	Whole blood taurine concentrations, plasma methionine concentrations, and urinary taurine:creatinine were also greater at days 14 and 28 compared with day 0 (P < 0.05), but no effect of diet was observed.
31943028	11	1	theme	taurine	1705:1711	arg1	concentrations					1713:1726	Whole blood taurine concentrations	1693:1726	Whole blood taurine concentrations	1693:1726	Whole blood taurine concentrations, plasma methionine concentrations, and urinary taurine:creatinine were also greater at days 14 and 28 compared with day 0 (P < 0.05), but no effect of diet was observed.
31943028	13	2	theme	higher	2214:2219	arg1	content					2221:2227	the higher content	2210:2227	the higher content of oligosaccharides and soluble fibers in the GF diet	2210:2281	In summary, overall taurine status was not affected by dietary treatments, however, our results suggest that the higher content of oligosaccharides and soluble fibers in the GF diet may alter the composition of the fecal bile acid pool.
31943028	1	3	theme	healthy	209:215	arg1	dogs					224:227	healthy Beagle dogs	209:227	healthy Beagle dogs	209:227	This study evaluated the effects of a grain-based (GB) and grain-free (GF) diet on protein utilization and taurine status in healthy Beagle dogs.
31943028	9	4	theme	Apparent	1342:1349	arg1	digestibility					1365:1377	Apparent crude protein digestibility	1342:1377	Apparent crude protein digestibility	1342:1377	Apparent crude protein digestibility was not affected by treatment, but dogs fed GF diet had lower apparent organic matter digestibility compared with those fed GB (P < 0.05).
31943028	2	5	from	protein	274:280	arg1	sufficient					254:263	sufficient	254:263	sufficient	254:263	Two practical dog diets sufficient in crude protein, sulfur amino acids, and taurine content were formulated with the same ingredients with exception of the carbohydrate sources.
31943028	12	6	theme	GF	1944:1945	arg1	groups					1954:1959	GF and GB groups	1944:1959	groups	1954:1959	Total bile acid excretion was similar between GF and GB groups, but dogs fed GF excreted a higher proportion of primary bile acids compared with those fed GB (25.49% vs. 12.09% at day 28, respectively).
31943028	6	7	theme	fresh	986:990	arg1	samples					998:1004	fresh fecal samples	986:1004	fresh fecal samples (primary and secondary bile acids)	986:1039	At the end of the adaptation period and every 2 wk after it (day 0, day 14, day 28), markers of taurine metabolism were analyzed in whole blood (taurine), plasma (taurine, methionine, and cystine), urine (taurine:creatinine), and fresh fecal samples (primary and secondary bile acids).
31943028	6	7	theme	fresh	986:990	arg1	acids					1034:1038	primary and secondary bile acids	1007:1038	primary and secondary bile acids	1007:1038	At the end of the adaptation period and every 2 wk after it (day 0, day 14, day 28), markers of taurine metabolism were analyzed in whole blood (taurine), plasma (taurine, methionine, and cystine), urine (taurine:creatinine), and fresh fecal samples (primary and secondary bile acids).
31943028	2	8	theme	practical	234:242	arg1	diets					248:252	Two practical dog diets	230:252	Two practical dog diets sufficient in crude protein, sulfur amino acids, and taurine content	230:321	Two practical dog diets sufficient in crude protein, sulfur amino acids, and taurine content were formulated with the same ingredients with exception of the carbohydrate sources.
31943028	7	9	theme	external	1169:1176	arg1	dioxide					1155:1161	titanium dioxide	1146:1161	titanium dioxide	1146:1161	Fecal samples were collected during the last 6 d of experimental period for digestibly assessment using titanium dioxide as an external marker.
31943028	7	9	theme	external	1169:1176	arg1	marker					1178:1183	an external marker	1166:1183	an external marker	1166:1183	Fecal samples were collected during the last 6 d of experimental period for digestibly assessment using titanium dioxide as an external marker.
31943028	9	10	theme	protein	1357:1363	arg1	digestibility					1365:1377	Apparent crude protein digestibility	1342:1377	Apparent crude protein digestibility	1342:1377	Apparent crude protein digestibility was not affected by treatment, but dogs fed GF diet had lower apparent organic matter digestibility compared with those fed GB (P < 0.05).
31943028	7	11	theme	digestibly	1118:1127	arg1	assessment					1129:1138	digestibly assessment	1118:1138	digestibly assessment using titanium dioxide as an external marker	1118:1183	Fecal samples were collected during the last 6 d of experimental period for digestibly assessment using titanium dioxide as an external marker.
31943028	4	12	with	design	578:583	arg1	six					590:592	six	590:592	six	590:592	A total of 12 Beagle dogs were used in a completely randomized design with six replicates per treatment.
31943028	1	13	theme	grain-free	143:152	arg1	diet					159:162	a grain-based (GB) and grain-free (GF) diet	120:162	diet	159:162	This study evaluated the effects of a grain-based (GB) and grain-free (GF) diet on protein utilization and taurine status in healthy Beagle dogs.
31943028	2	14	with	ingredients	353:363	arg1	exception					370:378	exception	370:378	exception of the carbohydrate sources	370:406	Two practical dog diets sufficient in crude protein, sulfur amino acids, and taurine content were formulated with the same ingredients with exception of the carbohydrate sources.
31943028	2	15	from	acids	296:300	arg1	sufficient					254:263	sufficient	254:263	sufficient	254:263	Two practical dog diets sufficient in crude protein, sulfur amino acids, and taurine content were formulated with the same ingredients with exception of the carbohydrate sources.
31943028	7	16	theme	period	1107:1112	arg1	d					1089:1089	the last 6 d	1078:1089	the last 6 d of experimental period for digestibly assessment using titanium dioxide as an external marker	1078:1183	Fecal samples were collected during the last 6 d of experimental period for digestibly assessment using titanium dioxide as an external marker.
31943028	1	17	from	status	199:204	arg1	dogs					224:227	healthy Beagle dogs	209:227	healthy Beagle dogs	209:227	This study evaluated the effects of a grain-based (GB) and grain-free (GF) diet on protein utilization and taurine status in healthy Beagle dogs.
31943028	13	18	theme	dietary	2156:2162	arg1	treatments					2164:2173	dietary treatments	2156:2173	dietary treatments	2156:2173	In summary, overall taurine status was not affected by dietary treatments, however, our results suggest that the higher content of oligosaccharides and soluble fibers in the GF diet may alter the composition of the fecal bile acid pool.
31943028	1	19	theme	GF	155:156	arg1	diet					159:162	a grain-based (GB) and grain-free (GF) diet	120:162	diet	159:162	This study evaluated the effects of a grain-based (GB) and grain-free (GF) diet on protein utilization and taurine status in healthy Beagle dogs.
31943028	4	20	theme	Beagle	529:534	arg1	dogs					536:539	12 Beagle dogs	526:539	12 Beagle dogs	526:539	A total of 12 Beagle dogs were used in a completely randomized design with six replicates per treatment.
31943028	8	21	from	GLIMMIX	1312:1318	arg1	version					1328:1334	version 9.4	1328:1338	version 9.4	1328:1338	Taurine markers and digestibility data were analyzed in a repeated measures model and one-way ANOVA, respectively, using PROC GLIMMIX in SAS (version 9.4).
31943028	8	21	from	GLIMMIX	1312:1318	arg1	SAS					1323:1325	SAS	1323:1325	SAS (version 9.4)	1323:1339	Taurine markers and digestibility data were analyzed in a repeated measures model and one-way ANOVA, respectively, using PROC GLIMMIX in SAS (version 9.4).
31943028	3	22	theme	tapioca	479:485	arg1	starch					487:492	tapioca starch	479:492	tapioca starch	479:492	The GB contained sorghum, millet, and spelt while potatoes, peas, and tapioca starch were used in the GF.
31943028	5	23	theme	d	713:713	arg1	period					700:705	an experimental period	684:705	an experimental period of 28 d in which GB and GF were fed to the dogs	684:753	The study consisted of an adaptation period of 2 wk followed by an experimental period of 28 d in which GB and GF were fed to the dogs.
31943028	12	24	theme	Total	1898:1902	arg1	excretion					1914:1922	Total bile acid excretion	1898:1922	Total bile acid excretion	1898:1922	Total bile acid excretion was similar between GF and GB groups, but dogs fed GF excreted a higher proportion of primary bile acids compared with those fed GB (25.49% vs. 12.09% at day 28, respectively).
31943028	12	25	theme	bile	2018:2021	arg1	acids					2023:2027	primary bile acids	2010:2027	primary bile acids	2010:2027	Total bile acid excretion was similar between GF and GB groups, but dogs fed GF excreted a higher proportion of primary bile acids compared with those fed GB (25.49% vs. 12.09% at day 28, respectively).
31943028	10	26	theme	greater	1639:1645	arg1	increase					1647:1654	greater increase	1639:1654	greater increase	1639:1654	Greater plasma taurine concentrations were observed at days 14 and 28 compared with day 0; wherein dogs fed GF exhibited greater increase compared to those fed GB (P < 0.05).
31943028	13	27	theme	bile	2322:2325	arg1	pool					2332:2335	the fecal bile acid pool	2312:2335	the fecal bile acid pool	2312:2335	In summary, overall taurine status was not affected by dietary treatments, however, our results suggest that the higher content of oligosaccharides and soluble fibers in the GF diet may alter the composition of the fecal bile acid pool.
31943028	1	28	theme	protein	167:173	arg1	utilization					175:185	protein utilization	167:185	protein utilization	167:185	This study evaluated the effects of a grain-based (GB) and grain-free (GF) diet on protein utilization and taurine status in healthy Beagle dogs.
31943028	6	29	theme	adaptation	774:783	arg1	period					785:790	the adaptation period	770:790	the adaptation period	770:790	At the end of the adaptation period and every 2 wk after it (day 0, day 14, day 28), markers of taurine metabolism were analyzed in whole blood (taurine), plasma (taurine, methionine, and cystine), urine (taurine:creatinine), and fresh fecal samples (primary and secondary bile acids).
31943028	5	30	dep	consisted	630:638	arg1	followed					672:679	followed	672:679	followed by an experimental period of 28 d in which GB and GF were fed to the dogs	672:753	The study consisted of an adaptation period of 2 wk followed by an experimental period of 28 d in which GB and GF were fed to the dogs.
31943028	12	31	theme	acid	1909:1912	arg1	excretion					1914:1922	Total bile acid excretion	1898:1922	Total bile acid excretion	1898:1922	Total bile acid excretion was similar between GF and GB groups, but dogs fed GF excreted a higher proportion of primary bile acids compared with those fed GB (25.49% vs. 12.09% at day 28, respectively).
31943028	13	32	theme	pool	2332:2335	arg1	composition					2297:2307	the composition	2293:2307	the composition of the fecal bile acid pool	2293:2335	In summary, overall taurine status was not affected by dietary treatments, however, our results suggest that the higher content of oligosaccharides and soluble fibers in the GF diet may alter the composition of the fecal bile acid pool.
31943028	6	33	theme	secondary	1019:1027	arg1	samples					998:1004	fresh fecal samples	986:1004	fresh fecal samples (primary and secondary bile acids)	986:1039	At the end of the adaptation period and every 2 wk after it (day 0, day 14, day 28), markers of taurine metabolism were analyzed in whole blood (taurine), plasma (taurine, methionine, and cystine), urine (taurine:creatinine), and fresh fecal samples (primary and secondary bile acids).
31943028	6	33	theme	secondary	1019:1027	arg1	acids					1034:1038	primary and secondary bile acids	1007:1038	primary and secondary bile acids	1007:1038	At the end of the adaptation period and every 2 wk after it (day 0, day 14, day 28), markers of taurine metabolism were analyzed in whole blood (taurine), plasma (taurine, methionine, and cystine), urine (taurine:creatinine), and fresh fecal samples (primary and secondary bile acids).
31943028	4	34	used	used	546:549	arg2	total					517:521	A total	515:521	A total of 12 Beagle dogs	515:539	A total of 12 Beagle dogs were used in a completely randomized design with six replicates per treatment.
31943028	13	35	theme	GF	2275:2276	arg1	diet					2278:2281	the GF diet	2271:2281	the GF diet	2271:2281	In summary, overall taurine status was not affected by dietary treatments, however, our results suggest that the higher content of oligosaccharides and soluble fibers in the GF diet may alter the composition of the fecal bile acid pool.
31943028	11	36	theme	Whole	1693:1697	arg1	concentrations					1713:1726	Whole blood taurine concentrations	1693:1726	Whole blood taurine concentrations	1693:1726	Whole blood taurine concentrations, plasma methionine concentrations, and urinary taurine:creatinine were also greater at days 14 and 28 compared with day 0 (P < 0.05), but no effect of diet was observed.
31943028	6	37	theme	primary	1007:1013	arg1	samples					998:1004	fresh fecal samples	986:1004	fresh fecal samples (primary and secondary bile acids)	986:1039	At the end of the adaptation period and every 2 wk after it (day 0, day 14, day 28), markers of taurine metabolism were analyzed in whole blood (taurine), plasma (taurine, methionine, and cystine), urine (taurine:creatinine), and fresh fecal samples (primary and secondary bile acids).
31943028	6	37	theme	primary	1007:1013	arg1	acids					1034:1038	primary and secondary bile acids	1007:1038	primary and secondary bile acids	1007:1038	At the end of the adaptation period and every 2 wk after it (day 0, day 14, day 28), markers of taurine metabolism were analyzed in whole blood (taurine), plasma (taurine, methionine, and cystine), urine (taurine:creatinine), and fresh fecal samples (primary and secondary bile acids).
31943028	8	38	theme	Taurine	1186:1192	arg1	markers					1194:1200	Taurine markers	1186:1200	Taurine markers	1186:1200	Taurine markers and digestibility data were analyzed in a repeated measures model and one-way ANOVA, respectively, using PROC GLIMMIX in SAS (version 9.4).
31943028	5	39	theme	adaptation	646:655	arg1	period					657:662	an adaptation period	643:662	an adaptation period of 2 wk	643:670	The study consisted of an adaptation period of 2 wk followed by an experimental period of 28 d in which GB and GF were fed to the dogs.
31943028	2	40	from	sufficient	254:263	arg1	content					315:321	taurine content	307:321	taurine content	307:321	Two practical dog diets sufficient in crude protein, sulfur amino acids, and taurine content were formulated with the same ingredients with exception of the carbohydrate sources.
31943028	2	40	from	sufficient	254:263	arg1	protein					274:280	crude protein	268:280	crude protein	268:280	Two practical dog diets sufficient in crude protein, sulfur amino acids, and taurine content were formulated with the same ingredients with exception of the carbohydrate sources.
31943028	2	40	from	sufficient	254:263	arg1	acids					296:300	sulfur amino acids	283:300	sulfur amino acids	283:300	Two practical dog diets sufficient in crude protein, sulfur amino acids, and taurine content were formulated with the same ingredients with exception of the carbohydrate sources.
31943028	9	41	theme	lower	1435:1439	arg1	digestibility					1465:1477	lower apparent organic matter digestibility	1435:1477	lower apparent organic matter digestibility	1435:1477	Apparent crude protein digestibility was not affected by treatment, but dogs fed GF diet had lower apparent organic matter digestibility compared with those fed GB (P < 0.05).
31943028	11	42	theme	blood	1699:1703	arg1	concentrations					1713:1726	Whole blood taurine concentrations	1693:1726	Whole blood taurine concentrations	1693:1726	Whole blood taurine concentrations, plasma methionine concentrations, and urinary taurine:creatinine were also greater at days 14 and 28 compared with day 0 (P < 0.05), but no effect of diet was observed.
31943028	10	43	theme	P	1682:1682	arg1	GB					1678:1679	those fed GB	1668:1679	those fed GB (P < 0.05)	1668:1690	Greater plasma taurine concentrations were observed at days 14 and 28 compared with day 0; wherein dogs fed GF exhibited greater increase compared to those fed GB (P < 0.05).
31943028	10	43	theme	P	1682:1682	arg1	<					1684:1684	P < 0.05	1682:1689	P < 0.05	1682:1689	Greater plasma taurine concentrations were observed at days 14 and 28 compared with day 0; wherein dogs fed GF exhibited greater increase compared to those fed GB (P < 0.05).
31943028	0	44	theme	Beagle	71:76	arg1	dogs					78:81	healthy Beagle dogs	63:81	healthy Beagle dogs	63:81	Effects of different carbohydrate sources on taurine status in healthy Beagle dogs.
31943028	11	45	theme	plasma	1729:1734	arg1	concentrations					1747:1760	plasma methionine concentrations	1729:1760	plasma methionine concentrations	1729:1760	Whole blood taurine concentrations, plasma methionine concentrations, and urinary taurine:creatinine were also greater at days 14 and 28 compared with day 0 (P < 0.05), but no effect of diet was observed.
31943028	9	46	theme	organic	1450:1456	arg1	digestibility					1465:1477	lower apparent organic matter digestibility	1435:1477	lower apparent organic matter digestibility	1435:1477	Apparent crude protein digestibility was not affected by treatment, but dogs fed GF diet had lower apparent organic matter digestibility compared with those fed GB (P < 0.05).
31943028	0	47	from	Effects	0:6	arg1	status					53:58	taurine status	45:58	taurine status in healthy Beagle dogs	45:81	Effects of different carbohydrate sources on taurine status in healthy Beagle dogs.
31943028	13	48	theme	overall	2113:2119	arg1	status					2129:2134	overall taurine status	2113:2134	overall taurine status	2113:2134	In summary, overall taurine status was not affected by dietary treatments, however, our results suggest that the higher content of oligosaccharides and soluble fibers in the GF diet may alter the composition of the fecal bile acid pool.
31943028	13	49	from	content	2221:2227	arg1	diet					2278:2281	the GF diet	2271:2281	the GF diet	2271:2281	In summary, overall taurine status was not affected by dietary treatments, however, our results suggest that the higher content of oligosaccharides and soluble fibers in the GF diet may alter the composition of the fecal bile acid pool.
31943028	3	50	contain	contained	416:424	arg2	spelt					447:451	spelt	447:451	spelt	447:451	The GB contained sorghum, millet, and spelt while potatoes, peas, and tapioca starch were used in the GF.
31943028	3	50	contain	contained	416:424	arg2	millet					435:440	millet	435:440	millet	435:440	The GB contained sorghum, millet, and spelt while potatoes, peas, and tapioca starch were used in the GF.
31943028	3	50	contain	contained	416:424	arg2	sorghum					426:432	sorghum	426:432	sorghum	426:432	The GB contained sorghum, millet, and spelt while potatoes, peas, and tapioca starch were used in the GF.
31943028	3	50	contain	contained	416:424	arg1	GB					413:414	The GB	409:414	The GB	409:414	The GB contained sorghum, millet, and spelt while potatoes, peas, and tapioca starch were used in the GF.
31943028	11	51	dep	greater	1804:1810	arg1	<					1853:1853	P < 0.05	1851:1858	P < 0.05	1851:1858	Whole blood taurine concentrations, plasma methionine concentrations, and urinary taurine:creatinine were also greater at days 14 and 28 compared with day 0 (P < 0.05), but no effect of diet was observed.
31943028	13	52	theme	fibers	2261:2266	arg1	content					2221:2227	the higher content	2210:2227	the higher content of oligosaccharides and soluble fibers in the GF diet	2210:2281	In summary, overall taurine status was not affected by dietary treatments, however, our results suggest that the higher content of oligosaccharides and soluble fibers in the GF diet may alter the composition of the fecal bile acid pool.
31943028	7	53	theme	Fecal	1042:1046	arg1	samples					1048:1054	Fecal samples	1042:1054	Fecal samples	1042:1054	Fecal samples were collected during the last 6 d of experimental period for digestibly assessment using titanium dioxide as an external marker.
31943028	2	54	theme	carbohydrate	387:398	arg1	sources					400:406	the carbohydrate sources	383:406	the carbohydrate sources	383:406	Two practical dog diets sufficient in crude protein, sulfur amino acids, and taurine content were formulated with the same ingredients with exception of the carbohydrate sources.
31943028	5	55	theme	experimental	687:698	arg1	period					700:705	an experimental period	684:705	an experimental period of 28 d in which GB and GF were fed to the dogs	684:753	The study consisted of an adaptation period of 2 wk followed by an experimental period of 28 d in which GB and GF were fed to the dogs.
31943028	11	56	theme	urinary	1767:1773	arg1	creatinine					1783:1792	urinary taurine:creatinine	1767:1792	urinary taurine:creatinine	1767:1792	Whole blood taurine concentrations, plasma methionine concentrations, and urinary taurine:creatinine were also greater at days 14 and 28 compared with day 0 (P < 0.05), but no effect of diet was observed.
31943028	9	57	theme	fed	1499:1501	arg1	GB					1503:1504	those fed GB	1493:1504	those fed GB (P < 0.05)	1493:1515	Apparent crude protein digestibility was not affected by treatment, but dogs fed GF diet had lower apparent organic matter digestibility compared with those fed GB (P < 0.05).
31943028	9	57	theme	fed	1499:1501	arg1	<					1509:1509	P < 0.05	1507:1514	P < 0.05	1507:1514	Apparent crude protein digestibility was not affected by treatment, but dogs fed GF diet had lower apparent organic matter digestibility compared with those fed GB (P < 0.05).
31943028	8	58	theme	measures	1253:1260	arg1	model					1262:1266	a repeated measures model	1242:1266	a repeated measures model	1242:1266	Taurine markers and digestibility data were analyzed in a repeated measures model and one-way ANOVA, respectively, using PROC GLIMMIX in SAS (version 9.4).
31943028	0	59	theme	different	11:19	arg1	sources					34:40	different carbohydrate sources	11:40	different carbohydrate sources	11:40	Effects of different carbohydrate sources on taurine status in healthy Beagle dogs.
31943028	12	60	theme	higher	1989:1994	arg1	proportion					1996:2005	a higher proportion	1987:2005	a higher proportion of primary bile acids	1987:2027	Total bile acid excretion was similar between GF and GB groups, but dogs fed GF excreted a higher proportion of primary bile acids compared with those fed GB (25.49% vs. 12.09% at day 28, respectively).
31943028	10	61	theme	Greater	1518:1524	arg1	concentrations					1541:1554	Greater plasma taurine concentrations	1518:1554	Greater plasma taurine concentrations	1518:1554	Greater plasma taurine concentrations were observed at days 14 and 28 compared with day 0; wherein dogs fed GF exhibited greater increase compared to those fed GB (P < 0.05).
31943028	2	62	theme	same	348:351	arg1	ingredients					353:363	the same ingredients	344:363	the same ingredients with exception of the carbohydrate sources	344:406	Two practical dog diets sufficient in crude protein, sulfur amino acids, and taurine content were formulated with the same ingredients with exception of the carbohydrate sources.
31943028	0	63	theme	sources	34:40	arg1	Effects					0:6	Effects	0:6	Effects of different carbohydrate sources on taurine status in healthy Beagle dogs	0:81	Effects of different carbohydrate sources on taurine status in healthy Beagle dogs.
31943028	5	64	theme	wk	669:670	arg1	period					657:662	an adaptation period	643:662	an adaptation period of 2 wk	643:670	The study consisted of an adaptation period of 2 wk followed by an experimental period of 28 d in which GB and GF were fed to the dogs.
31943028	2	65	theme	amino	290:294	arg1	acids					296:300	sulfur amino acids	283:300	sulfur amino acids	283:300	Two practical dog diets sufficient in crude protein, sulfur amino acids, and taurine content were formulated with the same ingredients with exception of the carbohydrate sources.
31943028	0	66	theme	taurine	45:51	arg1	status					53:58	taurine status	45:58	taurine status in healthy Beagle dogs	45:81	Effects of different carbohydrate sources on taurine status in healthy Beagle dogs.
31943028	10	67	located	observed	1561:1568	arg2	concentrations					1541:1554	Greater plasma taurine concentrations	1518:1554	Greater plasma taurine concentrations	1518:1554	Greater plasma taurine concentrations were observed at days 14 and 28 compared with day 0; wherein dogs fed GF exhibited greater increase compared to those fed GB (P < 0.05).
31943028	10	67	located	observed	1561:1568	arg1	days					1573:1576	days 14 and 28	1573:1586	days	1573:1576	Greater plasma taurine concentrations were observed at days 14 and 28 compared with day 0; wherein dogs fed GF exhibited greater increase compared to those fed GB (P < 0.05).
31943028	10	67	located	observed	1561:1568	arg1	28					1585:1586	28	1585:1586	28	1585:1586	Greater plasma taurine concentrations were observed at days 14 and 28 compared with day 0; wherein dogs fed GF exhibited greater increase compared to those fed GB (P < 0.05).
31943028	6	68	theme	whole	888:892	arg1	blood					894:898	whole blood	888:898	whole blood (taurine)	888:908	At the end of the adaptation period and every 2 wk after it (day 0, day 14, day 28), markers of taurine metabolism were analyzed in whole blood (taurine), plasma (taurine, methionine, and cystine), urine (taurine:creatinine), and fresh fecal samples (primary and secondary bile acids).
31943028	6	68	theme	whole	888:892	arg1	taurine					901:907	taurine	901:907	taurine	901:907	At the end of the adaptation period and every 2 wk after it (day 0, day 14, day 28), markers of taurine metabolism were analyzed in whole blood (taurine), plasma (taurine, methionine, and cystine), urine (taurine:creatinine), and fresh fecal samples (primary and secondary bile acids).
31943028	11	69	theme	diet	1879:1882	arg1	effect					1869:1874	no effect	1866:1874	no effect of diet	1866:1882	Whole blood taurine concentrations, plasma methionine concentrations, and urinary taurine:creatinine were also greater at days 14 and 28 compared with day 0 (P < 0.05), but no effect of diet was observed.
31943028	10	70	theme	taurine	1533:1539	arg1	concentrations					1541:1554	Greater plasma taurine concentrations	1518:1554	Greater plasma taurine concentrations	1518:1554	Greater plasma taurine concentrations were observed at days 14 and 28 compared with day 0; wherein dogs fed GF exhibited greater increase compared to those fed GB (P < 0.05).
31943028	2	71	theme	taurine	307:313	arg1	content					315:321	taurine content	307:321	taurine content	307:321	Two practical dog diets sufficient in crude protein, sulfur amino acids, and taurine content were formulated with the same ingredients with exception of the carbohydrate sources.
31943028	13	72	theme	soluble	2253:2259	arg1	fibers					2261:2266	soluble fibers	2253:2266	soluble fibers	2253:2266	In summary, overall taurine status was not affected by dietary treatments, however, our results suggest that the higher content of oligosaccharides and soluble fibers in the GF diet may alter the composition of the fecal bile acid pool.
31943028	1	73	theme	taurine	191:197	arg1	status					199:204	taurine status	191:204	taurine status	191:204	This study evaluated the effects of a grain-based (GB) and grain-free (GF) diet on protein utilization and taurine status in healthy Beagle dogs.
31943028	6	74	theme	metabolism	860:869	arg1	markers					841:847	markers	841:847	markers of taurine metabolism	841:869	At the end of the adaptation period and every 2 wk after it (day 0, day 14, day 28), markers of taurine metabolism were analyzed in whole blood (taurine), plasma (taurine, methionine, and cystine), urine (taurine:creatinine), and fresh fecal samples (primary and secondary bile acids).
31943028	6	75	theme	fecal	992:996	arg1	samples					998:1004	fresh fecal samples	986:1004	fresh fecal samples (primary and secondary bile acids)	986:1039	At the end of the adaptation period and every 2 wk after it (day 0, day 14, day 28), markers of taurine metabolism were analyzed in whole blood (taurine), plasma (taurine, methionine, and cystine), urine (taurine:creatinine), and fresh fecal samples (primary and secondary bile acids).
31943028	6	75	theme	fecal	992:996	arg1	acids					1034:1038	primary and secondary bile acids	1007:1038	primary and secondary bile acids	1007:1038	At the end of the adaptation period and every 2 wk after it (day 0, day 14, day 28), markers of taurine metabolism were analyzed in whole blood (taurine), plasma (taurine, methionine, and cystine), urine (taurine:creatinine), and fresh fecal samples (primary and secondary bile acids).
31943028	2	76	theme	dog	244:246	arg1	diets					248:252	Two practical dog diets	230:252	Two practical dog diets sufficient in crude protein, sulfur amino acids, and taurine content	230:321	Two practical dog diets sufficient in crude protein, sulfur amino acids, and taurine content were formulated with the same ingredients with exception of the carbohydrate sources.
31943028	13	77	dep	treatments	2164:2173	arg1	suggest					2197:2203	suggest	2197:2203	suggest that the higher content of oligosaccharides and soluble fibers in the GF diet may alter the composition of the fecal bile acid pool	2197:2335	In summary, overall taurine status was not affected by dietary treatments, however, our results suggest that the higher content of oligosaccharides and soluble fibers in the GF diet may alter the composition of the fecal bile acid pool.
31943028	1	78	theme	Beagle	217:222	arg1	dogs					224:227	healthy Beagle dogs	209:227	healthy Beagle dogs	209:227	This study evaluated the effects of a grain-based (GB) and grain-free (GF) diet on protein utilization and taurine status in healthy Beagle dogs.
31943028	9	79	theme	crude	1351:1355	arg1	digestibility					1365:1377	Apparent crude protein digestibility	1342:1377	Apparent crude protein digestibility	1342:1377	Apparent crude protein digestibility was not affected by treatment, but dogs fed GF diet had lower apparent organic matter digestibility compared with those fed GB (P < 0.05).
31943028	7	80	theme	titanium	1146:1153	arg1	dioxide					1155:1161	titanium dioxide	1146:1161	titanium dioxide	1146:1161	Fecal samples were collected during the last 6 d of experimental period for digestibly assessment using titanium dioxide as an external marker.
31943028	7	80	theme	titanium	1146:1153	arg1	marker					1178:1183	an external marker	1166:1183	an external marker	1166:1183	Fecal samples were collected during the last 6 d of experimental period for digestibly assessment using titanium dioxide as an external marker.
31943028	9	81	contain	had	1431:1433	arg1	dogs					1414:1417	dogs	1414:1417	dogs fed GF diet	1414:1429	Apparent crude protein digestibility was not affected by treatment, but dogs fed GF diet had lower apparent organic matter digestibility compared with those fed GB (P < 0.05).
31943028	9	81	contain	had	1431:1433	arg2	digestibility					1465:1477	lower apparent organic matter digestibility	1435:1477	lower apparent organic matter digestibility	1435:1477	Apparent crude protein digestibility was not affected by treatment, but dogs fed GF diet had lower apparent organic matter digestibility compared with those fed GB (P < 0.05).
31943028	12	82	theme	GB	1951:1952	arg1	groups					1954:1959	GF and GB groups	1944:1959	groups	1954:1959	Total bile acid excretion was similar between GF and GB groups, but dogs fed GF excreted a higher proportion of primary bile acids compared with those fed GB (25.49% vs. 12.09% at day 28, respectively).
31943028	11	83	theme	taurine	1775:1781	arg1	creatinine					1783:1792	urinary taurine:creatinine	1767:1792	urinary taurine:creatinine	1767:1792	Whole blood taurine concentrations, plasma methionine concentrations, and urinary taurine:creatinine were also greater at days 14 and 28 compared with day 0 (P < 0.05), but no effect of diet was observed.
31943028	12	84	theme	fed	2049:2051	arg1	GB					2053:2054	those fed GB	2043:2054	those fed GB	2043:2054	Total bile acid excretion was similar between GF and GB groups, but dogs fed GF excreted a higher proportion of primary bile acids compared with those fed GB (25.49% vs. 12.09% at day 28, respectively).
31943028	2	85	theme	crude	268:272	arg1	protein					274:280	crude protein	268:280	crude protein	268:280	Two practical dog diets sufficient in crude protein, sulfur amino acids, and taurine content were formulated with the same ingredients with exception of the carbohydrate sources.
31943028	12	86	theme	primary	2010:2016	arg1	acids					2023:2027	primary bile acids	2010:2027	primary bile acids	2010:2027	Total bile acid excretion was similar between GF and GB groups, but dogs fed GF excreted a higher proportion of primary bile acids compared with those fed GB (25.49% vs. 12.09% at day 28, respectively).
31943028	6	87	dep	plasma	911:916	arg1	methionine					928:937	methionine	928:937	methionine	928:937	At the end of the adaptation period and every 2 wk after it (day 0, day 14, day 28), markers of taurine metabolism were analyzed in whole blood (taurine), plasma (taurine, methionine, and cystine), urine (taurine:creatinine), and fresh fecal samples (primary and secondary bile acids).
31943028	6	87	dep	plasma	911:916	arg1	cystine					944:950	cystine	944:950	cystine	944:950	At the end of the adaptation period and every 2 wk after it (day 0, day 14, day 28), markers of taurine metabolism were analyzed in whole blood (taurine), plasma (taurine, methionine, and cystine), urine (taurine:creatinine), and fresh fecal samples (primary and secondary bile acids).
31943028	6	87	dep	plasma	911:916	arg1	taurine					919:925	taurine	919:925	taurine	919:925	At the end of the adaptation period and every 2 wk after it (day 0, day 14, day 28), markers of taurine metabolism were analyzed in whole blood (taurine), plasma (taurine, methionine, and cystine), urine (taurine:creatinine), and fresh fecal samples (primary and secondary bile acids).
31943028	10	88	theme	wherein	1609:1615	arg1	dogs					1617:1620	wherein dogs	1609:1620	wherein dogs fed GF	1609:1627	Greater plasma taurine concentrations were observed at days 14 and 28 compared with day 0; wherein dogs fed GF exhibited greater increase compared to those fed GB (P < 0.05).
31943028	2	89	theme	sufficient	254:263	arg1	diets					248:252	Two practical dog diets	230:252	Two practical dog diets sufficient in crude protein, sulfur amino acids, and taurine content	230:321	Two practical dog diets sufficient in crude protein, sulfur amino acids, and taurine content were formulated with the same ingredients with exception of the carbohydrate sources.
31943028	12	90	theme	bile	1904:1907	arg1	excretion					1914:1922	Total bile acid excretion	1898:1922	Total bile acid excretion	1898:1922	Total bile acid excretion was similar between GF and GB groups, but dogs fed GF excreted a higher proportion of primary bile acids compared with those fed GB (25.49% vs. 12.09% at day 28, respectively).
31943028	7	91	theme	experimental	1094:1105	arg1	period					1107:1112	experimental period	1094:1112	experimental period	1094:1112	Fecal samples were collected during the last 6 d of experimental period for digestibly assessment using titanium dioxide as an external marker.
31943028	12	92	theme	acids	2023:2027	arg1	proportion					1996:2005	a higher proportion	1987:2005	a higher proportion of primary bile acids	1987:2027	Total bile acid excretion was similar between GF and GB groups, but dogs fed GF excreted a higher proportion of primary bile acids compared with those fed GB (25.49% vs. 12.09% at day 28, respectively).
31943028	6	93	theme	period	785:790	arg1	wk					804:805	2 wk	802:805	every 2 wk after it (day 0, day 14, day 28)	796:838	At the end of the adaptation period and every 2 wk after it (day 0, day 14, day 28), markers of taurine metabolism were analyzed in whole blood (taurine), plasma (taurine, methionine, and cystine), urine (taurine:creatinine), and fresh fecal samples (primary and secondary bile acids).
31943028	6	93	theme	period	785:790	arg1	end					763:765	the end	759:765	the end of the adaptation period	759:790	At the end of the adaptation period and every 2 wk after it (day 0, day 14, day 28), markers of taurine metabolism were analyzed in whole blood (taurine), plasma (taurine, methionine, and cystine), urine (taurine:creatinine), and fresh fecal samples (primary and secondary bile acids).
31943028	4	94	theme	dogs	536:539	arg1	total					517:521	A total	515:521	A total of 12 Beagle dogs	515:539	A total of 12 Beagle dogs were used in a completely randomized design with six replicates per treatment.
31943028	6	95	theme	bile	1029:1032	arg1	samples					998:1004	fresh fecal samples	986:1004	fresh fecal samples (primary and secondary bile acids)	986:1039	At the end of the adaptation period and every 2 wk after it (day 0, day 14, day 28), markers of taurine metabolism were analyzed in whole blood (taurine), plasma (taurine, methionine, and cystine), urine (taurine:creatinine), and fresh fecal samples (primary and secondary bile acids).
31943028	6	95	theme	bile	1029:1032	arg1	acids					1034:1038	primary and secondary bile acids	1007:1038	primary and secondary bile acids	1007:1038	At the end of the adaptation period and every 2 wk after it (day 0, day 14, day 28), markers of taurine metabolism were analyzed in whole blood (taurine), plasma (taurine, methionine, and cystine), urine (taurine:creatinine), and fresh fecal samples (primary and secondary bile acids).
31943028	13	96	theme	acid	2327:2330	arg1	pool					2332:2335	the fecal bile acid pool	2312:2335	the fecal bile acid pool	2312:2335	In summary, overall taurine status was not affected by dietary treatments, however, our results suggest that the higher content of oligosaccharides and soluble fibers in the GF diet may alter the composition of the fecal bile acid pool.
31943028	7	97	theme	last	1082:1085	arg1	d					1089:1089	the last 6 d	1078:1089	the last 6 d of experimental period for digestibly assessment using titanium dioxide as an external marker	1078:1183	Fecal samples were collected during the last 6 d of experimental period for digestibly assessment using titanium dioxide as an external marker.
31943028	3	98	used	used	499:502	arg2	potatoes					459:466	potatoes	459:466	potatoes	459:466	The GB contained sorghum, millet, and spelt while potatoes, peas, and tapioca starch were used in the GF.
31943028	3	98	used	used	499:502	arg2	starch					487:492	tapioca starch	479:492	tapioca starch	479:492	The GB contained sorghum, millet, and spelt while potatoes, peas, and tapioca starch were used in the GF.
31943028	3	98	used	used	499:502	arg2	peas					469:472	peas	469:472	peas	469:472	The GB contained sorghum, millet, and spelt while potatoes, peas, and tapioca starch were used in the GF.
31943028	1	99	from	utilization	175:185	arg1	dogs					224:227	healthy Beagle dogs	209:227	healthy Beagle dogs	209:227	This study evaluated the effects of a grain-based (GB) and grain-free (GF) diet on protein utilization and taurine status in healthy Beagle dogs.
31943028	9	100	theme	GF	1423:1424	arg1	diet					1426:1429	GF diet	1423:1429	GF diet	1423:1429	Apparent crude protein digestibility was not affected by treatment, but dogs fed GF diet had lower apparent organic matter digestibility compared with those fed GB (P < 0.05).
31943028	6	101	dep	wk	804:805	arg1	day					817:819	day 0	817:821	day 0	817:821	At the end of the adaptation period and every 2 wk after it (day 0, day 14, day 28), markers of taurine metabolism were analyzed in whole blood (taurine), plasma (taurine, methionine, and cystine), urine (taurine:creatinine), and fresh fecal samples (primary and secondary bile acids).
31943028	6	101	dep	wk	804:805	arg1	day					824:826	day 14	824:829	day 14	824:829	At the end of the adaptation period and every 2 wk after it (day 0, day 14, day 28), markers of taurine metabolism were analyzed in whole blood (taurine), plasma (taurine, methionine, and cystine), urine (taurine:creatinine), and fresh fecal samples (primary and secondary bile acids).
31943028	6	101	dep	wk	804:805	arg1	day					832:834	day 28	832:837	day 28	832:837	At the end of the adaptation period and every 2 wk after it (day 0, day 14, day 28), markers of taurine metabolism were analyzed in whole blood (taurine), plasma (taurine, methionine, and cystine), urine (taurine:creatinine), and fresh fecal samples (primary and secondary bile acids).
31943028	0	102	theme	healthy	63:69	arg1	dogs					78:81	healthy Beagle dogs	63:81	healthy Beagle dogs	63:81	Effects of different carbohydrate sources on taurine status in healthy Beagle dogs.
31943028	1	103	theme	grain-based	122:132	arg1	GB					135:136	a grain-based (GB) and grain-free (GF) diet	120:162	GB	135:136	This study evaluated the effects of a grain-based (GB) and grain-free (GF) diet on protein utilization and taurine status in healthy Beagle dogs.
31943028	13	104	theme	oligosaccharides	2232:2247	arg1	content					2221:2227	the higher content	2210:2227	the higher content of oligosaccharides and soluble fibers in the GF diet	2210:2281	In summary, overall taurine status was not affected by dietary treatments, however, our results suggest that the higher content of oligosaccharides and soluble fibers in the GF diet may alter the composition of the fecal bile acid pool.
31943028	8	105	theme	digestibility	1206:1218	arg1	data					1220:1223	digestibility data	1206:1223	digestibility data	1206:1223	Taurine markers and digestibility data were analyzed in a repeated measures model and one-way ANOVA, respectively, using PROC GLIMMIX in SAS (version 9.4).
31943028	4	106	theme	randomized	567:576	arg1	design					578:583	a completely randomized design	554:583	a completely randomized design with six replicates per treatment	554:617	A total of 12 Beagle dogs were used in a completely randomized design with six replicates per treatment.
31943028	11	107	theme	P	1851:1851	arg1	<					1853:1853	P < 0.05	1851:1858	P < 0.05	1851:1858	Whole blood taurine concentrations, plasma methionine concentrations, and urinary taurine:creatinine were also greater at days 14 and 28 compared with day 0 (P < 0.05), but no effect of diet was observed.
31943028	13	108	theme	fecal	2316:2320	arg1	pool					2332:2335	the fecal bile acid pool	2312:2335	the fecal bile acid pool	2312:2335	In summary, overall taurine status was not affected by dietary treatments, however, our results suggest that the higher content of oligosaccharides and soluble fibers in the GF diet may alter the composition of the fecal bile acid pool.
31943028	1	109	theme	GB	135:136	arg1	effects					109:115	the effects	105:115	the effects of a grain-based (GB) and grain-free (GF) diet on protein utilization and taurine status in healthy Beagle dogs	105:227	This study evaluated the effects of a grain-based (GB) and grain-free (GF) diet on protein utilization and taurine status in healthy Beagle dogs.
31943028	9	110	theme	apparent	1441:1448	arg1	digestibility					1465:1477	lower apparent organic matter digestibility	1435:1477	lower apparent organic matter digestibility	1435:1477	Apparent crude protein digestibility was not affected by treatment, but dogs fed GF diet had lower apparent organic matter digestibility compared with those fed GB (P < 0.05).
31943028	6	111	dep	taurine	961:967	arg1	creatinine					969:978	creatinine	969:978	creatinine	969:978	At the end of the adaptation period and every 2 wk after it (day 0, day 14, day 28), markers of taurine metabolism were analyzed in whole blood (taurine), plasma (taurine, methionine, and cystine), urine (taurine:creatinine), and fresh fecal samples (primary and secondary bile acids).
31943028	4	112	dep	six	590:592	arg1	replicates					594:603	replicates	594:603	replicates	594:603	A total of 12 Beagle dogs were used in a completely randomized design with six replicates per treatment.
31943028	9	113	theme	matter	1458:1463	arg1	digestibility					1465:1477	lower apparent organic matter digestibility	1435:1477	lower apparent organic matter digestibility	1435:1477	Apparent crude protein digestibility was not affected by treatment, but dogs fed GF diet had lower apparent organic matter digestibility compared with those fed GB (P < 0.05).
31943028	0	114	from	status	53:58	arg1	dogs					78:81	healthy Beagle dogs	63:81	healthy Beagle dogs	63:81	Effects of different carbohydrate sources on taurine status in healthy Beagle dogs.
31943028	10	115	theme	fed	1674:1676	arg1	GB					1678:1679	those fed GB	1668:1679	those fed GB (P < 0.05)	1668:1690	Greater plasma taurine concentrations were observed at days 14 and 28 compared with day 0; wherein dogs fed GF exhibited greater increase compared to those fed GB (P < 0.05).
31943028	10	115	theme	fed	1674:1676	arg1	<					1684:1684	P < 0.05	1682:1689	P < 0.05	1682:1689	Greater plasma taurine concentrations were observed at days 14 and 28 compared with day 0; wherein dogs fed GF exhibited greater increase compared to those fed GB (P < 0.05).
31943028	13	116	theme	taurine	2121:2127	arg1	status					2129:2134	overall taurine status	2113:2134	overall taurine status	2113:2134	In summary, overall taurine status was not affected by dietary treatments, however, our results suggest that the higher content of oligosaccharides and soluble fibers in the GF diet may alter the composition of the fecal bile acid pool.
31943028	1	117	theme	diet	159:162	arg1	effects					109:115	the effects	105:115	the effects of a grain-based (GB) and grain-free (GF) diet on protein utilization and taurine status in healthy Beagle dogs	105:227	This study evaluated the effects of a grain-based (GB) and grain-free (GF) diet on protein utilization and taurine status in healthy Beagle dogs.
31943028	6	118	theme	taurine	852:858	arg1	metabolism					860:869	taurine metabolism	852:869	taurine metabolism	852:869	At the end of the adaptation period and every 2 wk after it (day 0, day 14, day 28), markers of taurine metabolism were analyzed in whole blood (taurine), plasma (taurine, methionine, and cystine), urine (taurine:creatinine), and fresh fecal samples (primary and secondary bile acids).
31943028	8	119	theme	repeated	1244:1251	arg1	model					1262:1266	a repeated measures model	1242:1266	a repeated measures model	1242:1266	Taurine markers and digestibility data were analyzed in a repeated measures model and one-way ANOVA, respectively, using PROC GLIMMIX in SAS (version 9.4).
31943028	12	120	dep	excreted	1978:1985	arg1	%					2062:2062	25.49%	2057:2062	25.49%	2057:2062	Total bile acid excretion was similar between GF and GB groups, but dogs fed GF excreted a higher proportion of primary bile acids compared with those fed GB (25.49% vs. 12.09% at day 28, respectively).
31943028	12	120	dep	excreted	1978:1985	arg1	%					2073:2073	12.09%	2068:2073	12.09% at day 28	2068:2083	Total bile acid excretion was similar between GF and GB groups, but dogs fed GF excreted a higher proportion of primary bile acids compared with those fed GB (25.49% vs. 12.09% at day 28, respectively).
31943028	0	121	theme	carbohydrate	21:32	arg1	sources					34:40	different carbohydrate sources	11:40	different carbohydrate sources	11:40	Effects of different carbohydrate sources on taurine status in healthy Beagle dogs.
31943028	1	122	from	effects	109:115	arg1	utilization					175:185	protein utilization	167:185	protein utilization	167:185	This study evaluated the effects of a grain-based (GB) and grain-free (GF) diet on protein utilization and taurine status in healthy Beagle dogs.
31943028	1	122	from	effects	109:115	arg1	status					199:204	taurine status	191:204	taurine status	191:204	This study evaluated the effects of a grain-based (GB) and grain-free (GF) diet on protein utilization and taurine status in healthy Beagle dogs.
31943028	8	123	theme	one-way	1272:1278	arg1	ANOVA					1280:1284	one-way ANOVA	1272:1284	one-way ANOVA	1272:1284	Taurine markers and digestibility data were analyzed in a repeated measures model and one-way ANOVA, respectively, using PROC GLIMMIX in SAS (version 9.4).
31943028	10	124	theme	plasma	1526:1531	arg1	concentrations					1541:1554	Greater plasma taurine concentrations	1518:1554	Greater plasma taurine concentrations	1518:1554	Greater plasma taurine concentrations were observed at days 14 and 28 compared with day 0; wherein dogs fed GF exhibited greater increase compared to those fed GB (P < 0.05).
31943028	9	125	theme	P	1507:1507	arg1	GB					1503:1504	those fed GB	1493:1504	those fed GB (P < 0.05)	1493:1515	Apparent crude protein digestibility was not affected by treatment, but dogs fed GF diet had lower apparent organic matter digestibility compared with those fed GB (P < 0.05).
31943028	9	125	theme	P	1507:1507	arg1	<					1509:1509	P < 0.05	1507:1514	P < 0.05	1507:1514	Apparent crude protein digestibility was not affected by treatment, but dogs fed GF diet had lower apparent organic matter digestibility compared with those fed GB (P < 0.05).
31943028	2	126	from	content	315:321	arg1	sufficient					254:263	sufficient	254:263	sufficient	254:263	Two practical dog diets sufficient in crude protein, sulfur amino acids, and taurine content were formulated with the same ingredients with exception of the carbohydrate sources.
31943028	2	127	theme	sources	400:406	arg1	exception					370:378	exception	370:378	exception of the carbohydrate sources	370:406	Two practical dog diets sufficient in crude protein, sulfur amino acids, and taurine content were formulated with the same ingredients with exception of the carbohydrate sources.
31943028	2	128	theme	sulfur	283:288	arg1	acids					296:300	sulfur amino acids	283:300	sulfur amino acids	283:300	Two practical dog diets sufficient in crude protein, sulfur amino acids, and taurine content were formulated with the same ingredients with exception of the carbohydrate sources.
31943028	8	129	theme	PROC	1307:1310	arg1	GLIMMIX					1312:1318	PROC GLIMMIX	1307:1318	PROC GLIMMIX in SAS (version 9.4)	1307:1339	Taurine markers and digestibility data were analyzed in a repeated measures model and one-way ANOVA, respectively, using PROC GLIMMIX in SAS (version 9.4).
31943028	12	130	from	day	2078:2080	arg1	%					2073:2073	12.09%	2068:2073	12.09% at day 28	2068:2083	Total bile acid excretion was similar between GF and GB groups, but dogs fed GF excreted a higher proportion of primary bile acids compared with those fed GB (25.49% vs. 12.09% at day 28, respectively).
33761275	3	0	theme	photochemical	487:499	arg1	method					512:517	The photochemical deposition method	483:517	The photochemical deposition method	483:517	The photochemical deposition method was used to synthesize the nano-Ag0/TiO2-CS antiviral material.
33761275	6	1	theme	reduction	949:957	arg1	assay					959:963	the MS2 plaque reduction assay	934:963	the MS2 plaque reduction assay (PRA)	934:969	The antiviral efficacy of nano-Ag0/TiO2-CS was evaluated by the MS2 plaque reduction assay (PRA) and filtration experiments.
33761275	6	1	theme	reduction	949:957	arg1	PRA					966:968	PRA	966:968	PRA	966:968	The antiviral efficacy of nano-Ag0/TiO2-CS was evaluated by the MS2 plaque reduction assay (PRA) and filtration experiments.
33761275	11	2	theme	infection	1669:1677	arg1	probability					1679:1689	airborne infection probability	1660:1689	airborne infection probability	1660:1689	The Wells-Riley model predicted that when the nano-Ag0/TiO2-CS filter was used in the ventilation system, airborne infection probability would reduce from 99% to 34.6%.
33761275	7	3	theme	optical	1150:1156	arg1	particle					1158:1165	an optical particle counter	1147:1173	an optical particle counter	1147:1173	In the filtration experiments, the MS2 aerosols passed through the nano-Ag0/TiO2-CS filter, and the MS2 aerosol removal efficiency was evaluated by an optical particle counter and culture method.
33761275	6	4	theme	MS2	938:940	arg1	assay					959:963	the MS2 plaque reduction assay	934:963	the MS2 plaque reduction assay (PRA)	934:969	The antiviral efficacy of nano-Ag0/TiO2-CS was evaluated by the MS2 plaque reduction assay (PRA) and filtration experiments.
33761275	6	4	theme	MS2	938:940	arg1	PRA					966:968	PRA	966:968	PRA	966:968	The antiviral efficacy of nano-Ag0/TiO2-CS was evaluated by the MS2 plaque reduction assay (PRA) and filtration experiments.
33761275	3	5	used	used	523:526	arg2	method					512:517	The photochemical deposition method	483:517	The photochemical deposition method	483:517	The photochemical deposition method was used to synthesize the nano-Ag0/TiO2-CS antiviral material.
33761275	11	6	theme	Wells-Riley	1558:1568	arg1	model					1570:1574	The Wells-Riley model	1554:1574	The Wells-Riley model	1554:1574	The Wells-Riley model predicted that when the nano-Ag0/TiO2-CS filter was used in the ventilation system, airborne infection probability would reduce from 99% to 34.6%.
33761275	8	7	theme	20 mL	1276:1280	arg1	culture					1289:1295	20 mL liquid culture	1276:1295	20 mL liquid culture (2 ± 0.5 × 1016 PFU/mL)	1276:1319	In the MS2 PRA, 3 g of nano-Ag0/TiO2-CS inactivated 97% of MS2 bacteriophages in 20 mL liquid culture (2 ± 0.5 × 1016 PFU/mL) within 2 hours.
33761275	8	7	theme	20 mL	1276:1280	arg1	PFU/mL					1313:1318	2 ± 0.5 × 1016 PFU/mL	1298:1318	2 ± 0.5 × 1016 PFU/mL	1298:1318	In the MS2 PRA, 3 g of nano-Ag0/TiO2-CS inactivated 97% of MS2 bacteriophages in 20 mL liquid culture (2 ± 0.5 × 1016 PFU/mL) within 2 hours.
33761275	2	8	theme	nanosilver/titania-chitosan	271:297	arg1	bed					325:327	a zero-valent nanosilver/titania-chitosan (nano-Ag0/TiO2-CS) filter bed	257:327	a zero-valent nanosilver/titania-chitosan (nano-Ag0/TiO2-CS) filter bed	257:327	In this study, we applied a zero-valent nanosilver/titania-chitosan (nano-Ag0/TiO2-CS) filter bed, whose broad-spectrum antimicrobial efficacy has been proven previously, for the removal of viral aerosols to minimize the risk of airborne transmission.
33761275	8	9	theme	nano-Ag0/TiO2-CS	1218:1233	arg1	3 g					1211:1213	3 g	1211:1213	3 g of nano-Ag0/TiO2-CS	1211:1233	In the MS2 PRA, 3 g of nano-Ag0/TiO2-CS inactivated 97% of MS2 bacteriophages in 20 mL liquid culture (2 ± 0.5 × 1016 PFU/mL) within 2 hours.
33761275	12	10	theme	nano-Ag0/TiO2-CS	1727:1742	arg1	filter					1744:1749	The nano-Ag0/TiO2-CS filter	1723:1749	The nano-Ag0/TiO2-CS filter	1723:1749	The nano-Ag0/TiO2-CS filter could remain at 50% of its original antiviral efficiency after continuous operation for 1 week, indicating its feasibility for the control of the airborne transmission.
33761275	7	11	theme	removal	1111:1117	arg1	efficiency					1119:1128	the MS2 aerosol removal efficiency	1095:1128	the MS2 aerosol removal efficiency	1095:1128	In the filtration experiments, the MS2 aerosols passed through the nano-Ag0/TiO2-CS filter, and the MS2 aerosol removal efficiency was evaluated by an optical particle counter and culture method.
33761275	4	12	dep	morphology	595:604	arg1	The					583:585	The	583:585	The	583:585	The surface morphology, elemental composition, and microstructure of the nano-Ag0/TiO2-CS were analyzed by a scanning electron microscopy/energy dispersive X-ray spectroscopy and a transmission electron microscopy, respectively.
33761275	3	13	theme	nano-Ag0/TiO2-CS	546:561	arg1	material					573:580	the nano-Ag0/TiO2-CS antiviral material	542:580	the nano-Ag0/TiO2-CS antiviral material	542:580	The photochemical deposition method was used to synthesize the nano-Ag0/TiO2-CS antiviral material.
33761275	7	14	theme	MS2	1099:1101	arg1	efficiency					1119:1128	the MS2 aerosol removal efficiency	1095:1128	the MS2 aerosol removal efficiency	1095:1128	In the filtration experiments, the MS2 aerosols passed through the nano-Ag0/TiO2-CS filter, and the MS2 aerosol removal efficiency was evaluated by an optical particle counter and culture method.
33761275	5	15	theme	viral	858:862	arg1	bacteriophages					820:833	The MS2 bacteriophages	812:833	The MS2 bacteriophages	812:833	The MS2 bacteriophages were used as surrogate viral aerosols.
33761275	5	15	theme	viral	858:862	arg1	aerosols					864:871	surrogate viral aerosols	848:871	surrogate viral aerosols	848:871	The MS2 bacteriophages were used as surrogate viral aerosols.
33761275	10	16	from	%	1448:1448	arg1	surface					1479:1485	the surface	1475:1485	the surface of the nano-Ag0/TiO2-CS filter	1475:1516	Over 95% of MS2 bacteriophages on the surface of the nano-Ag0/TiO2-CS filter were inactivated within 20 minutes.
33761275	4	17	theme	X-ray	739:743	arg1	spectroscopy					745:756	a scanning electron microscopy/energy dispersive X-ray spectroscopy	690:756	a scanning electron microscopy/energy dispersive X-ray spectroscopy	690:756	The surface morphology, elemental composition, and microstructure of the nano-Ag0/TiO2-CS were analyzed by a scanning electron microscopy/energy dispersive X-ray spectroscopy and a transmission electron microscopy, respectively.
33761275	1	18	theme	transmission	124:135	arg1	precaution					101:110	The precaution	97:110	The precaution of airborne transmission of viruses, such as influenza, SARS, MERS, and COVID-19,	97:192	The precaution of airborne transmission of viruses, such as influenza, SARS, MERS, and COVID-19, is essential for reducing infection.
33761275	1	18	theme	transmission	124:135	arg1	essential					197:205	essential	197:205	essential	197:205	The precaution of airborne transmission of viruses, such as influenza, SARS, MERS, and COVID-19, is essential for reducing infection.
33761275	9	19	dep	93	1436:1437	arg1	to					1433:1434	to	1433:1434	to	1433:1434	The removal efficiency of nano-Ag0/TiO2-CS filter (thickness: 6 cm) for MS2 aerosols reached up to 93%.
33761275	1	20	theme	viruses	140:146	arg1	precaution					101:110	The precaution	97:110	The precaution of airborne transmission of viruses, such as influenza, SARS, MERS, and COVID-19,	97:192	The precaution of airborne transmission of viruses, such as influenza, SARS, MERS, and COVID-19, is essential for reducing infection.
33761275	1	20	theme	viruses	140:146	arg1	essential					197:205	essential	197:205	essential	197:205	The precaution of airborne transmission of viruses, such as influenza, SARS, MERS, and COVID-19, is essential for reducing infection.
33761275	4	21	theme	scanning	692:699	arg1	spectroscopy					745:756	a scanning electron microscopy/energy dispersive X-ray spectroscopy	690:756	a scanning electron microscopy/energy dispersive X-ray spectroscopy	690:756	The surface morphology, elemental composition, and microstructure of the nano-Ag0/TiO2-CS were analyzed by a scanning electron microscopy/energy dispersive X-ray spectroscopy and a transmission electron microscopy, respectively.
33761275	2	22	theme	airborne	460:467	arg1	transmission					469:480	airborne transmission	460:480	airborne transmission	460:480	In this study, we applied a zero-valent nanosilver/titania-chitosan (nano-Ag0/TiO2-CS) filter bed, whose broad-spectrum antimicrobial efficacy has been proven previously, for the removal of viral aerosols to minimize the risk of airborne transmission.
33761275	9	23	dep	filter	1380:1385	arg1	thickness					1388:1396	thickness	1388:1396	thickness: 6 cm	1388:1402	The removal efficiency of nano-Ag0/TiO2-CS filter (thickness: 6 cm) for MS2 aerosols reached up to 93%.
33761275	4	24	theme	microscopy/energy	710:726	arg1	spectroscopy					745:756	a scanning electron microscopy/energy dispersive X-ray spectroscopy	690:756	a scanning electron microscopy/energy dispersive X-ray spectroscopy	690:756	The surface morphology, elemental composition, and microstructure of the nano-Ag0/TiO2-CS were analyzed by a scanning electron microscopy/energy dispersive X-ray spectroscopy and a transmission electron microscopy, respectively.
33761275	0	25	theme	Viral	81:85	arg1	Aerosols					87:94	Viral Aerosols	81:94	Viral Aerosols	81:94	Effectiveness of the Nanosilver/TiO2-Chitosan Antiviral Filter on the Removal of Viral Aerosols.
33761275	0	26	from	Effectiveness	0:12	arg1	Removal					70:76	the Removal	66:76	the Removal of Viral Aerosols	66:94	Effectiveness of the Nanosilver/TiO2-Chitosan Antiviral Filter on the Removal of Viral Aerosols.
33761275	12	27	theme	transmission	1906:1917	arg1	control					1882:1888	the control	1878:1888	the control of the airborne transmission	1878:1917	The nano-Ag0/TiO2-CS filter could remain at 50% of its original antiviral efficiency after continuous operation for 1 week, indicating its feasibility for the control of the airborne transmission.
33761275	10	28	theme	bacteriophages	1457:1470	arg1	%					1448:1448	95%	1446:1448	Over 95% of MS2 bacteriophages on the surface of the nano-Ag0/TiO2-CS filter	1441:1516	Over 95% of MS2 bacteriophages on the surface of the nano-Ag0/TiO2-CS filter were inactivated within 20 minutes.
33761275	10	28	theme	bacteriophages	1457:1470	arg1	bacteriophages					1457:1470	MS2 bacteriophages	1453:1470	MS2 bacteriophages	1453:1470	Over 95% of MS2 bacteriophages on the surface of the nano-Ag0/TiO2-CS filter were inactivated within 20 minutes.
33761275	0	29	theme	Filter	56:61	arg1	Effectiveness					0:12	Effectiveness	0:12	Effectiveness of the Nanosilver/TiO2-Chitosan Antiviral Filter on the Removal of Viral Aerosols.	0:95	Effectiveness of the Nanosilver/TiO2-Chitosan Antiviral Filter on the Removal of Viral Aerosols.
33761275	2	30	theme	aerosols	427:434	arg1	removal					410:416	the removal	406:416	the removal of viral aerosols	406:434	In this study, we applied a zero-valent nanosilver/titania-chitosan (nano-Ag0/TiO2-CS) filter bed, whose broad-spectrum antimicrobial efficacy has been proven previously, for the removal of viral aerosols to minimize the risk of airborne transmission.
33761275	9	31	dep	thickness	1388:1396	arg1	6 cm					1399:1402	6 cm	1399:1402	thickness: 6 cm	1388:1402	The removal efficiency of nano-Ag0/TiO2-CS filter (thickness: 6 cm) for MS2 aerosols reached up to 93%.
33761275	4	32	theme	transmission	764:775	arg1	microscopy					786:795	a transmission electron microscopy	762:795	a transmission electron microscopy	762:795	The surface morphology, elemental composition, and microstructure of the nano-Ag0/TiO2-CS were analyzed by a scanning electron microscopy/energy dispersive X-ray spectroscopy and a transmission electron microscopy, respectively.
33761275	5	33	theme	surrogate	848:856	arg1	bacteriophages					820:833	The MS2 bacteriophages	812:833	The MS2 bacteriophages	812:833	The MS2 bacteriophages were used as surrogate viral aerosols.
33761275	5	33	theme	surrogate	848:856	arg1	aerosols					864:871	surrogate viral aerosols	848:871	surrogate viral aerosols	848:871	The MS2 bacteriophages were used as surrogate viral aerosols.
33761275	9	34	theme	nano-Ag0/TiO2-CS	1363:1378	arg1	filter					1380:1385	nano-Ag0/TiO2-CS filter	1363:1385	nano-Ag0/TiO2-CS filter (thickness: 6 cm)	1363:1403	The removal efficiency of nano-Ag0/TiO2-CS filter (thickness: 6 cm) for MS2 aerosols reached up to 93%.
33761275	11	35	theme	99	1709:1710	arg1	%					1711:1711	%	1711:1711	%	1711:1711	The Wells-Riley model predicted that when the nano-Ag0/TiO2-CS filter was used in the ventilation system, airborne infection probability would reduce from 99% to 34.6%.
33761275	8	36	theme	bacteriophages	1258:1271	arg1	%					1249:1249	97%	1247:1249	97% of MS2 bacteriophages	1247:1271	In the MS2 PRA, 3 g of nano-Ag0/TiO2-CS inactivated 97% of MS2 bacteriophages in 20 mL liquid culture (2 ± 0.5 × 1016 PFU/mL) within 2 hours.
33761275	8	36	theme	bacteriophages	1258:1271	arg1	bacteriophages					1258:1271	MS2 bacteriophages	1254:1271	MS2 bacteriophages	1254:1271	In the MS2 PRA, 3 g of nano-Ag0/TiO2-CS inactivated 97% of MS2 bacteriophages in 20 mL liquid culture (2 ± 0.5 × 1016 PFU/mL) within 2 hours.
33761275	0	37	theme	Antiviral	46:54	arg1	Filter					56:61	the Nanosilver/TiO2-Chitosan Antiviral Filter	17:61	the Nanosilver/TiO2-Chitosan Antiviral Filter	17:61	Effectiveness of the Nanosilver/TiO2-Chitosan Antiviral Filter on the Removal of Viral Aerosols.
33761275	6	38	theme	nano-Ag0/TiO2-CS	900:915	arg1	efficacy					888:895	The antiviral efficacy	874:895	The antiviral efficacy of nano-Ag0/TiO2-CS	874:915	The antiviral efficacy of nano-Ag0/TiO2-CS was evaluated by the MS2 plaque reduction assay (PRA) and filtration experiments.
33761275	12	39	theme	efficiency	1797:1806	arg1	efficiency					1797:1806	its original antiviral efficiency	1774:1806	its original antiviral efficiency	1774:1806	The nano-Ag0/TiO2-CS filter could remain at 50% of its original antiviral efficiency after continuous operation for 1 week, indicating its feasibility for the control of the airborne transmission.
33761275	12	39	theme	efficiency	1797:1806	arg1	%					1769:1769	50%	1767:1769	50% of its original antiviral efficiency	1767:1806	The nano-Ag0/TiO2-CS filter could remain at 50% of its original antiviral efficiency after continuous operation for 1 week, indicating its feasibility for the control of the airborne transmission.
33761275	7	40	theme	culture	1179:1185	arg1	method					1187:1192	culture method	1179:1192	culture method	1179:1192	In the filtration experiments, the MS2 aerosols passed through the nano-Ag0/TiO2-CS filter, and the MS2 aerosol removal efficiency was evaluated by an optical particle counter and culture method.
33761275	12	41	theme	continuous	1814:1823	arg1	operation					1825:1833	continuous operation	1814:1833	continuous operation for 1 week	1814:1844	The nano-Ag0/TiO2-CS filter could remain at 50% of its original antiviral efficiency after continuous operation for 1 week, indicating its feasibility for the control of the airborne transmission.
33761275	10	42	theme	nano-Ag0/TiO2-CS	1494:1509	arg1	filter					1511:1516	the nano-Ag0/TiO2-CS filter	1490:1516	the nano-Ag0/TiO2-CS filter	1490:1516	Over 95% of MS2 bacteriophages on the surface of the nano-Ag0/TiO2-CS filter were inactivated within 20 minutes.
33761275	2	43	theme	antimicrobial	351:363	arg1	efficacy					365:372	efficacy	365:372	efficacy	365:372	In this study, we applied a zero-valent nanosilver/titania-chitosan (nano-Ag0/TiO2-CS) filter bed, whose broad-spectrum antimicrobial efficacy has been proven previously, for the removal of viral aerosols to minimize the risk of airborne transmission.
33761275	8	44	theme	MS2	1254:1256	arg1	bacteriophages					1258:1271	MS2 bacteriophages	1254:1271	MS2 bacteriophages	1254:1271	In the MS2 PRA, 3 g of nano-Ag0/TiO2-CS inactivated 97% of MS2 bacteriophages in 20 mL liquid culture (2 ± 0.5 × 1016 PFU/mL) within 2 hours.
33761275	9	45	theme	MS2	1409:1411	arg1	aerosols					1413:1420	MS2 aerosols	1409:1420	MS2 aerosols	1409:1420	The removal efficiency of nano-Ag0/TiO2-CS filter (thickness: 6 cm) for MS2 aerosols reached up to 93%.
33761275	12	46	theme	original	1778:1785	arg1	efficiency					1797:1806	its original antiviral efficiency	1774:1806	its original antiviral efficiency	1774:1806	The nano-Ag0/TiO2-CS filter could remain at 50% of its original antiviral efficiency after continuous operation for 1 week, indicating its feasibility for the control of the airborne transmission.
33761275	11	47	dep	%	1711:1711	arg1	to					1713:1714	to	1713:1714	to	1713:1714	The Wells-Riley model predicted that when the nano-Ag0/TiO2-CS filter was used in the ventilation system, airborne infection probability would reduce from 99% to 34.6%.
33761275	6	48	theme	plaque	942:947	arg1	assay					959:963	the MS2 plaque reduction assay	934:963	the MS2 plaque reduction assay (PRA)	934:969	The antiviral efficacy of nano-Ag0/TiO2-CS was evaluated by the MS2 plaque reduction assay (PRA) and filtration experiments.
33761275	6	48	theme	plaque	942:947	arg1	PRA					966:968	PRA	966:968	PRA	966:968	The antiviral efficacy of nano-Ag0/TiO2-CS was evaluated by the MS2 plaque reduction assay (PRA) and filtration experiments.
33761275	4	49	theme	surface	587:593	arg1	morphology					595:604	surface morphology	587:604	surface morphology	587:604	The surface morphology, elemental composition, and microstructure of the nano-Ag0/TiO2-CS were analyzed by a scanning electron microscopy/energy dispersive X-ray spectroscopy and a transmission electron microscopy, respectively.
33761275	8	50	theme	MS2	1202:1204	arg1	PRA					1206:1208	the MS2 PRA	1198:1208	the MS2 PRA	1198:1208	In the MS2 PRA, 3 g of nano-Ag0/TiO2-CS inactivated 97% of MS2 bacteriophages in 20 mL liquid culture (2 ± 0.5 × 1016 PFU/mL) within 2 hours.
33761275	4	51	theme	nano-Ag0/TiO2-CS	656:671	arg1	morphology					595:604	surface morphology	587:604	surface morphology	587:604	The surface morphology, elemental composition, and microstructure of the nano-Ag0/TiO2-CS were analyzed by a scanning electron microscopy/energy dispersive X-ray spectroscopy and a transmission electron microscopy, respectively.
33761275	4	51	theme	nano-Ag0/TiO2-CS	656:671	arg1	composition					617:627	elemental composition	607:627	elemental composition	607:627	The surface morphology, elemental composition, and microstructure of the nano-Ag0/TiO2-CS were analyzed by a scanning electron microscopy/energy dispersive X-ray spectroscopy and a transmission electron microscopy, respectively.
33761275	4	51	theme	nano-Ag0/TiO2-CS	656:671	arg1	microstructure					634:647	microstructure	634:647	microstructure	634:647	The surface morphology, elemental composition, and microstructure of the nano-Ag0/TiO2-CS were analyzed by a scanning electron microscopy/energy dispersive X-ray spectroscopy and a transmission electron microscopy, respectively.
33761275	11	52	theme	airborne	1660:1667	arg1	probability					1679:1689	airborne infection probability	1660:1689	airborne infection probability	1660:1689	The Wells-Riley model predicted that when the nano-Ag0/TiO2-CS filter was used in the ventilation system, airborne infection probability would reduce from 99% to 34.6%.
33761275	3	53	theme	antiviral	563:571	arg1	material					573:580	the nano-Ag0/TiO2-CS antiviral material	542:580	the nano-Ag0/TiO2-CS antiviral material	542:580	The photochemical deposition method was used to synthesize the nano-Ag0/TiO2-CS antiviral material.
33761275	7	54	dep	particle	1158:1165	arg1	counter					1167:1173	counter	1167:1173	counter	1167:1173	In the filtration experiments, the MS2 aerosols passed through the nano-Ag0/TiO2-CS filter, and the MS2 aerosol removal efficiency was evaluated by an optical particle counter and culture method.
33761275	7	55	theme	aerosol	1103:1109	arg1	efficiency					1119:1128	the MS2 aerosol removal efficiency	1095:1128	the MS2 aerosol removal efficiency	1095:1128	In the filtration experiments, the MS2 aerosols passed through the nano-Ag0/TiO2-CS filter, and the MS2 aerosol removal efficiency was evaluated by an optical particle counter and culture method.
33761275	2	56	theme	zero-valent	259:269	arg1	bed					325:327	a zero-valent nanosilver/titania-chitosan (nano-Ag0/TiO2-CS) filter bed	257:327	a zero-valent nanosilver/titania-chitosan (nano-Ag0/TiO2-CS) filter bed	257:327	In this study, we applied a zero-valent nanosilver/titania-chitosan (nano-Ag0/TiO2-CS) filter bed, whose broad-spectrum antimicrobial efficacy has been proven previously, for the removal of viral aerosols to minimize the risk of airborne transmission.
33761275	8	57	theme	liquid	1282:1287	arg1	culture					1289:1295	20 mL liquid culture	1276:1295	20 mL liquid culture (2 ± 0.5 × 1016 PFU/mL)	1276:1319	In the MS2 PRA, 3 g of nano-Ag0/TiO2-CS inactivated 97% of MS2 bacteriophages in 20 mL liquid culture (2 ± 0.5 × 1016 PFU/mL) within 2 hours.
33761275	8	57	theme	liquid	1282:1287	arg1	PFU/mL					1313:1318	2 ± 0.5 × 1016 PFU/mL	1298:1318	2 ± 0.5 × 1016 PFU/mL	1298:1318	In the MS2 PRA, 3 g of nano-Ag0/TiO2-CS inactivated 97% of MS2 bacteriophages in 20 mL liquid culture (2 ± 0.5 × 1016 PFU/mL) within 2 hours.
33761275	1	58	theme	airborne	115:122	arg1	transmission					124:135	airborne transmission	115:135	airborne transmission	115:135	The precaution of airborne transmission of viruses, such as influenza, SARS, MERS, and COVID-19, is essential for reducing infection.
33761275	4	59	theme	dispersive	728:737	arg1	spectroscopy					745:756	a scanning electron microscopy/energy dispersive X-ray spectroscopy	690:756	a scanning electron microscopy/energy dispersive X-ray spectroscopy	690:756	The surface morphology, elemental composition, and microstructure of the nano-Ag0/TiO2-CS were analyzed by a scanning electron microscopy/energy dispersive X-ray spectroscopy and a transmission electron microscopy, respectively.
33761275	7	60	theme	nano-Ag0/TiO2-CS	1066:1081	arg1	filter					1083:1088	the nano-Ag0/TiO2-CS filter	1062:1088	the nano-Ag0/TiO2-CS filter	1062:1088	In the filtration experiments, the MS2 aerosols passed through the nano-Ag0/TiO2-CS filter, and the MS2 aerosol removal efficiency was evaluated by an optical particle counter and culture method.
33761275	7	61	theme	MS2	1034:1036	arg1	aerosols					1038:1045	the MS2 aerosols	1030:1045	the MS2 aerosols	1030:1045	In the filtration experiments, the MS2 aerosols passed through the nano-Ag0/TiO2-CS filter, and the MS2 aerosol removal efficiency was evaluated by an optical particle counter and culture method.
33761275	2	62	theme	transmission	469:480	arg1	risk					452:455	the risk	448:455	the risk of airborne transmission	448:480	In this study, we applied a zero-valent nanosilver/titania-chitosan (nano-Ag0/TiO2-CS) filter bed, whose broad-spectrum antimicrobial efficacy has been proven previously, for the removal of viral aerosols to minimize the risk of airborne transmission.
33761275	0	63	theme	Aerosols	87:94	arg1	Removal					70:76	the Removal	66:76	the Removal of Viral Aerosols	66:94	Effectiveness of the Nanosilver/TiO2-Chitosan Antiviral Filter on the Removal of Viral Aerosols.
33761275	12	64	theme	airborne	1897:1904	arg1	transmission					1906:1917	the airborne transmission	1893:1917	the airborne transmission	1893:1917	The nano-Ag0/TiO2-CS filter could remain at 50% of its original antiviral efficiency after continuous operation for 1 week, indicating its feasibility for the control of the airborne transmission.
33761275	10	65	theme	Over	1441:1444	arg1	%					1448:1448	95%	1446:1448	Over 95% of MS2 bacteriophages on the surface of the nano-Ag0/TiO2-CS filter	1441:1516	Over 95% of MS2 bacteriophages on the surface of the nano-Ag0/TiO2-CS filter were inactivated within 20 minutes.
33761275	10	65	theme	Over	1441:1444	arg1	bacteriophages					1457:1470	MS2 bacteriophages	1453:1470	MS2 bacteriophages	1453:1470	Over 95% of MS2 bacteriophages on the surface of the nano-Ag0/TiO2-CS filter were inactivated within 20 minutes.
33761275	4	66	theme	electron	701:708	arg1	spectroscopy					745:756	a scanning electron microscopy/energy dispersive X-ray spectroscopy	690:756	a scanning electron microscopy/energy dispersive X-ray spectroscopy	690:756	The surface morphology, elemental composition, and microstructure of the nano-Ag0/TiO2-CS were analyzed by a scanning electron microscopy/energy dispersive X-ray spectroscopy and a transmission electron microscopy, respectively.
33761275	7	67	theme	filtration	1006:1015	arg1	experiments					1017:1027	the filtration experiments	1002:1027	the filtration experiments	1002:1027	In the filtration experiments, the MS2 aerosols passed through the nano-Ag0/TiO2-CS filter, and the MS2 aerosol removal efficiency was evaluated by an optical particle counter and culture method.
33761275	10	68	theme	MS2	1453:1455	arg1	bacteriophages					1457:1470	MS2 bacteriophages	1453:1470	MS2 bacteriophages	1453:1470	Over 95% of MS2 bacteriophages on the surface of the nano-Ag0/TiO2-CS filter were inactivated within 20 minutes.
33761275	9	69	theme	removal	1341:1347	arg1	efficiency					1349:1358	The removal efficiency	1337:1358	The removal efficiency of nano-Ag0/TiO2-CS filter (thickness: 6 cm) for MS2 aerosols	1337:1420	The removal efficiency of nano-Ag0/TiO2-CS filter (thickness: 6 cm) for MS2 aerosols reached up to 93%.
33761275	2	70	theme	viral	421:425	arg1	aerosols					427:434	viral aerosols	421:434	viral aerosols	421:434	In this study, we applied a zero-valent nanosilver/titania-chitosan (nano-Ag0/TiO2-CS) filter bed, whose broad-spectrum antimicrobial efficacy has been proven previously, for the removal of viral aerosols to minimize the risk of airborne transmission.
33761275	0	71	theme	Nanosilver/TiO2-Chitosan	21:44	arg1	Filter					56:61	the Nanosilver/TiO2-Chitosan Antiviral Filter	17:61	the Nanosilver/TiO2-Chitosan Antiviral Filter	17:61	Effectiveness of the Nanosilver/TiO2-Chitosan Antiviral Filter on the Removal of Viral Aerosols.
33761275	5	72	used	used	840:843	arg2	aerosols					864:871	surrogate viral aerosols	848:871	surrogate viral aerosols	848:871	The MS2 bacteriophages were used as surrogate viral aerosols.
33761275	5	72	used	used	840:843	arg2	bacteriophages					820:833	The MS2 bacteriophages	812:833	The MS2 bacteriophages	812:833	The MS2 bacteriophages were used as surrogate viral aerosols.
33761275	4	73	theme	electron	777:784	arg1	microscopy					786:795	a transmission electron microscopy	762:795	a transmission electron microscopy	762:795	The surface morphology, elemental composition, and microstructure of the nano-Ag0/TiO2-CS were analyzed by a scanning electron microscopy/energy dispersive X-ray spectroscopy and a transmission electron microscopy, respectively.
33761275	3	74	theme	deposition	501:510	arg1	method					512:517	The photochemical deposition method	483:517	The photochemical deposition method	483:517	The photochemical deposition method was used to synthesize the nano-Ag0/TiO2-CS antiviral material.
33761275	5	75	theme	MS2	816:818	arg1	bacteriophages					820:833	The MS2 bacteriophages	812:833	The MS2 bacteriophages	812:833	The MS2 bacteriophages were used as surrogate viral aerosols.
33761275	5	75	theme	MS2	816:818	arg1	aerosols					864:871	surrogate viral aerosols	848:871	surrogate viral aerosols	848:871	The MS2 bacteriophages were used as surrogate viral aerosols.
33761275	9	76	theme	filter	1380:1385	arg1	efficiency					1349:1358	The removal efficiency	1337:1358	The removal efficiency of nano-Ag0/TiO2-CS filter (thickness: 6 cm) for MS2 aerosols	1337:1420	The removal efficiency of nano-Ag0/TiO2-CS filter (thickness: 6 cm) for MS2 aerosols reached up to 93%.
33761275	11	77	used	used	1628:1631	arg2	filter					1617:1622	the nano-Ag0/TiO2-CS filter	1596:1622	the nano-Ag0/TiO2-CS filter	1596:1622	The Wells-Riley model predicted that when the nano-Ag0/TiO2-CS filter was used in the ventilation system, airborne infection probability would reduce from 99% to 34.6%.
33761275	12	78	theme	antiviral	1787:1795	arg1	efficiency					1797:1806	its original antiviral efficiency	1774:1806	its original antiviral efficiency	1774:1806	The nano-Ag0/TiO2-CS filter could remain at 50% of its original antiviral efficiency after continuous operation for 1 week, indicating its feasibility for the control of the airborne transmission.
33761275	10	79	theme	filter	1511:1516	arg1	surface					1479:1485	the surface	1475:1485	the surface of the nano-Ag0/TiO2-CS filter	1475:1516	Over 95% of MS2 bacteriophages on the surface of the nano-Ag0/TiO2-CS filter were inactivated within 20 minutes.
33761275	11	80	theme	34.6	1716:1719	arg1	%					1711:1711	%	1711:1711	%	1711:1711	The Wells-Riley model predicted that when the nano-Ag0/TiO2-CS filter was used in the ventilation system, airborne infection probability would reduce from 99% to 34.6%.
33761275	2	81	theme	filter	318:323	arg1	bed					325:327	a zero-valent nanosilver/titania-chitosan (nano-Ag0/TiO2-CS) filter bed	257:327	a zero-valent nanosilver/titania-chitosan (nano-Ag0/TiO2-CS) filter bed	257:327	In this study, we applied a zero-valent nanosilver/titania-chitosan (nano-Ag0/TiO2-CS) filter bed, whose broad-spectrum antimicrobial efficacy has been proven previously, for the removal of viral aerosols to minimize the risk of airborne transmission.
33761275	6	82	theme	antiviral	878:886	arg1	efficacy					888:895	The antiviral efficacy	874:895	The antiviral efficacy of nano-Ag0/TiO2-CS	874:915	The antiviral efficacy of nano-Ag0/TiO2-CS was evaluated by the MS2 plaque reduction assay (PRA) and filtration experiments.
33761275	4	83	theme	elemental	607:615	arg1	composition					617:627	elemental composition	607:627	elemental composition	607:627	The surface morphology, elemental composition, and microstructure of the nano-Ag0/TiO2-CS were analyzed by a scanning electron microscopy/energy dispersive X-ray spectroscopy and a transmission electron microscopy, respectively.
33761275	2	84	theme	nano-Ag0/TiO2-CS	300:315	arg1	bed					325:327	a zero-valent nanosilver/titania-chitosan (nano-Ag0/TiO2-CS) filter bed	257:327	a zero-valent nanosilver/titania-chitosan (nano-Ag0/TiO2-CS) filter bed	257:327	In this study, we applied a zero-valent nanosilver/titania-chitosan (nano-Ag0/TiO2-CS) filter bed, whose broad-spectrum antimicrobial efficacy has been proven previously, for the removal of viral aerosols to minimize the risk of airborne transmission.
33761275	11	85	theme	ventilation	1640:1650	arg1	system					1652:1657	the ventilation system	1636:1657	the ventilation system	1636:1657	The Wells-Riley model predicted that when the nano-Ag0/TiO2-CS filter was used in the ventilation system, airborne infection probability would reduce from 99% to 34.6%.
33761275	11	86	theme	nano-Ag0/TiO2-CS	1600:1615	arg1	filter					1617:1622	the nano-Ag0/TiO2-CS filter	1596:1622	the nano-Ag0/TiO2-CS filter	1596:1622	The Wells-Riley model predicted that when the nano-Ag0/TiO2-CS filter was used in the ventilation system, airborne infection probability would reduce from 99% to 34.6%.
33761275	2	87	theme	broad-spectrum	336:349	arg1	efficacy					365:372	efficacy	365:372	efficacy	365:372	In this study, we applied a zero-valent nanosilver/titania-chitosan (nano-Ag0/TiO2-CS) filter bed, whose broad-spectrum antimicrobial efficacy has been proven previously, for the removal of viral aerosols to minimize the risk of airborne transmission.
33761275	6	88	theme	filtration	975:984	arg1	experiments					986:996	filtration experiments	975:996	filtration experiments	975:996	The antiviral efficacy of nano-Ag0/TiO2-CS was evaluated by the MS2 plaque reduction assay (PRA) and filtration experiments.
31909867	11	0	theme	promising	1519:1527	arg1	biomaterial					1529:1539	a promising biomaterial	1517:1539	a promising biomaterial for use on implant surfaces	1517:1567	The modified NT surfaces with tanfloc/heparin polyelectrolyte multilayers are a promising biomaterial for use on implant surfaces because of their enhanced blood biocompatibility and antibacterial properties.
31909867	11	0	theme	promising	1519:1527	arg1	surfaces					1455:1462	The modified NT surfaces	1439:1462	The modified NT surfaces with tanfloc/heparin polyelectrolyte multilayers	1439:1511	The modified NT surfaces with tanfloc/heparin polyelectrolyte multilayers are a promising biomaterial for use on implant surfaces because of their enhanced blood biocompatibility and antibacterial properties.
31909867	10	1	theme	aeruginosa	1351:1360	arg1	adhesion					1318:1325	adhesion	1318:1325	adhesion	1318:1325	Modifying NT with tanfloc/heparin also reduces the adhesion and proliferation of P. aeruginosa and S. aureus bacteria after 24 hr of incubation, with no biofilm formation.
31909867	10	1	theme	aeruginosa	1351:1360	arg1	proliferation					1331:1343	proliferation	1331:1343	proliferation	1331:1343	Modifying NT with tanfloc/heparin also reduces the adhesion and proliferation of P. aeruginosa and S. aureus bacteria after 24 hr of incubation, with no biofilm formation.
31909867	0	2	theme	polyelectrolyte	96:110	arg1	multilayers					112:122	tanfloc/heparin polyelectrolyte multilayers	80:122	tanfloc/heparin polyelectrolyte multilayers	80:122	Enhanced hemocompatibility and antibacterial activity on titania nanotubes with tanfloc/heparin polyelectrolyte multilayers.
31909867	0	3	from	hemocompatibility	9:25	arg1	titania					57:63	titania	57:63	titania	57:63	Enhanced hemocompatibility and antibacterial activity on titania nanotubes with tanfloc/heparin polyelectrolyte multilayers.
31909867	11	4	with	surfaces	1455:1462	arg1	multilayers					1501:1511	tanfloc/heparin polyelectrolyte multilayers	1469:1511	tanfloc/heparin polyelectrolyte multilayers	1469:1511	The modified NT surfaces with tanfloc/heparin polyelectrolyte multilayers are a promising biomaterial for use on implant surfaces because of their enhanced blood biocompatibility and antibacterial properties.
31909867	1	5	theme	blood-contacting	219:234	arg1	devices					236:242	blood-contacting devices	219:242	blood-contacting devices	219:242	Biomaterial-associated thrombus formation and bacterial infection remain major challenges for blood-contacting devices.
31909867	8	6	theme	Bacterial	970:978	arg1	adhesion					980:987	adhesion	980:987	adhesion	980:987	Bacterial adhesion and morphology, as well as biofilm formation, were analyzed using fluorescence microscopy and SEM.
31909867	11	7	theme	enhanced	1586:1593	arg1	biocompatibility					1601:1616	their enhanced blood biocompatibility	1580:1616	their enhanced blood biocompatibility	1580:1616	The modified NT surfaces with tanfloc/heparin polyelectrolyte multilayers are a promising biomaterial for use on implant surfaces because of their enhanced blood biocompatibility and antibacterial properties.
31909867	4	8	theme	polyelectrolyte	478:492	arg1	multilayers					494:504	tanfloc/heparin polyelectrolyte multilayers	462:504	tanfloc/heparin polyelectrolyte multilayers on titania nanotubes array surfaces (NT)	462:545	To address these challenges, tanfloc/heparin polyelectrolyte multilayers on titania nanotubes array surfaces (NT) were developed.
31909867	4	9	theme	titania	509:515	arg1	surfaces					533:540	titania nanotubes array surfaces	509:540	titania nanotubes array surfaces (NT)	509:545	To address these challenges, tanfloc/heparin polyelectrolyte multilayers on titania nanotubes array surfaces (NT) were developed.
31909867	4	9	theme	titania	509:515	arg1	NT					543:544	NT	543:544	NT	543:544	To address these challenges, tanfloc/heparin polyelectrolyte multilayers on titania nanotubes array surfaces (NT) were developed.
31909867	8	10	theme	biofilm	1016:1022	arg1	formation					1024:1032	biofilm formation	1016:1032	biofilm formation	1016:1032	Bacterial adhesion and morphology, as well as biofilm formation, were analyzed using fluorescence microscopy and SEM.
31909867	5	11	theme	contact	684:690	arg1	measurements					698:709	water contact angle measurements	678:709	water contact angle measurements	678:709	The surfaces were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and water contact angle measurements.
31909867	9	12	theme	platelet	1233:1240	arg1	adhesion					1242:1249	platelet adhesion	1233:1249	platelet adhesion	1233:1249	The anti-thrombogenic properties of the surfaces were demonstrated by significant decreases in fibrinogen adsorption, Factor XII activation, and platelet adhesion and activation.
31909867	10	13	theme	biofilm	1420:1426	arg1	formation					1428:1436	no biofilm formation	1417:1436	no biofilm formation	1417:1436	Modifying NT with tanfloc/heparin also reduces the adhesion and proliferation of P. aeruginosa and S. aureus bacteria after 24 hr of incubation, with no biofilm formation.
31909867	5	14	theme	angle	692:696	arg1	measurements					698:709	water contact angle measurements	678:709	water contact angle measurements	678:709	The surfaces were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and water contact angle measurements.
31909867	4	15	from	multilayers	494:504	arg1	surfaces					533:540	titania nanotubes array surfaces	509:540	titania nanotubes array surfaces (NT)	509:545	To address these challenges, tanfloc/heparin polyelectrolyte multilayers on titania nanotubes array surfaces (NT) were developed.
31909867	4	15	from	multilayers	494:504	arg1	NT					543:544	NT	543:544	NT	543:544	To address these challenges, tanfloc/heparin polyelectrolyte multilayers on titania nanotubes array surfaces (NT) were developed.
31909867	6	16	theme	XII	793:795	arg1	activation					797:806	Factor XII activation	786:806	Factor XII activation	786:806	To evaluate the hemocompatibility of the surfaces, fibrinogen adsorption, Factor XII activation, and platelet adhesion and activation were analyzed.
31909867	11	17	theme	implant	1552:1558	arg1	surfaces					1560:1567	implant surfaces	1552:1567	implant surfaces	1552:1567	The modified NT surfaces with tanfloc/heparin polyelectrolyte multilayers are a promising biomaterial for use on implant surfaces because of their enhanced blood biocompatibility and antibacterial properties.
31909867	11	18	theme	modified	1443:1450	arg1	surfaces					1455:1462	The modified NT surfaces	1439:1462	The modified NT surfaces with tanfloc/heparin polyelectrolyte multilayers	1439:1511	The modified NT surfaces with tanfloc/heparin polyelectrolyte multilayers are a promising biomaterial for use on implant surfaces because of their enhanced blood biocompatibility and antibacterial properties.
31909867	11	18	theme	modified	1443:1450	arg1	biomaterial					1529:1539	a promising biomaterial	1517:1539	a promising biomaterial for use on implant surfaces	1517:1567	The modified NT surfaces with tanfloc/heparin polyelectrolyte multilayers are a promising biomaterial for use on implant surfaces because of their enhanced blood biocompatibility and antibacterial properties.
31909867	11	19	theme	tanfloc/heparin	1469:1483	arg1	multilayers					1501:1511	tanfloc/heparin polyelectrolyte multilayers	1469:1511	tanfloc/heparin polyelectrolyte multilayers	1469:1511	The modified NT surfaces with tanfloc/heparin polyelectrolyte multilayers are a promising biomaterial for use on implant surfaces because of their enhanced blood biocompatibility and antibacterial properties.
31909867	5	20	theme	scanning	598:605	arg1	SEM					628:630	SEM	628:630	SEM	628:630	The surfaces were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and water contact angle measurements.
31909867	5	20	theme	scanning	598:605	arg1	microscopy					616:625	scanning electron microscopy	598:625	scanning electron microscopy (SEM)	598:631	The surfaces were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and water contact angle measurements.
31909867	11	21	theme	blood	1595:1599	arg1	biocompatibility					1601:1616	their enhanced blood biocompatibility	1580:1616	their enhanced blood biocompatibility	1580:1616	The modified NT surfaces with tanfloc/heparin polyelectrolyte multilayers are a promising biomaterial for use on implant surfaces because of their enhanced blood biocompatibility and antibacterial properties.
31909867	0	22	from	activity	45:52	arg1	titania					57:63	titania	57:63	titania	57:63	Enhanced hemocompatibility and antibacterial activity on titania nanotubes with tanfloc/heparin polyelectrolyte multilayers.
31909867	5	23	theme	electron	607:614	arg1	SEM					628:630	SEM	628:630	SEM	628:630	The surfaces were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and water contact angle measurements.
31909867	5	23	theme	electron	607:614	arg1	microscopy					616:625	scanning electron microscopy	598:625	scanning electron microscopy (SEM)	598:631	The surfaces were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and water contact angle measurements.
31909867	0	24	theme	Enhanced	0:7	arg1	hemocompatibility					9:25	Enhanced hemocompatibility	0:25	Enhanced hemocompatibility	0:25	Enhanced hemocompatibility and antibacterial activity on titania nanotubes with tanfloc/heparin polyelectrolyte multilayers.
31909867	9	25	theme	XII	1213:1215	arg1	activation					1217:1226	Factor XII activation	1206:1226	Factor XII activation	1206:1226	The anti-thrombogenic properties of the surfaces were demonstrated by significant decreases in fibrinogen adsorption, Factor XII activation, and platelet adhesion and activation.
31909867	4	26	theme	tanfloc/heparin	462:476	arg1	multilayers					494:504	tanfloc/heparin polyelectrolyte multilayers	462:504	tanfloc/heparin polyelectrolyte multilayers on titania nanotubes array surfaces (NT)	462:545	To address these challenges, tanfloc/heparin polyelectrolyte multilayers on titania nanotubes array surfaces (NT) were developed.
31909867	1	27	theme	Biomaterial-associated	125:146	arg1	formation					157:165	Biomaterial-associated thrombus formation	125:165	Biomaterial-associated thrombus formation	125:165	Biomaterial-associated thrombus formation and bacterial infection remain major challenges for blood-contacting devices.
31909867	11	28	theme	antibacterial	1622:1634	arg1	properties					1636:1645	antibacterial properties	1622:1645	antibacterial properties	1622:1645	The modified NT surfaces with tanfloc/heparin polyelectrolyte multilayers are a promising biomaterial for use on implant surfaces because of their enhanced blood biocompatibility and antibacterial properties.
31909867	0	29	theme	antibacterial	31:43	arg1	activity					45:52	antibacterial activity	31:52	antibacterial activity	31:52	Enhanced hemocompatibility and antibacterial activity on titania nanotubes with tanfloc/heparin polyelectrolyte multilayers.
31909867	10	30	theme	no	1417:1418	arg1	formation					1428:1436	no biofilm formation	1417:1436	no biofilm formation	1417:1436	Modifying NT with tanfloc/heparin also reduces the adhesion and proliferation of P. aeruginosa and S. aureus bacteria after 24 hr of incubation, with no biofilm formation.
31909867	1	31	theme	thrombus	148:155	arg1	formation					157:165	Biomaterial-associated thrombus formation	125:165	Biomaterial-associated thrombus formation	125:165	Biomaterial-associated thrombus formation and bacterial infection remain major challenges for blood-contacting devices.
31909867	6	32	theme	fibrinogen	763:772	arg1	adsorption					774:783	fibrinogen adsorption	763:783	fibrinogen adsorption	763:783	To evaluate the hemocompatibility of the surfaces, fibrinogen adsorption, Factor XII activation, and platelet adhesion and activation were analyzed.
31909867	9	33	theme	anti-thrombogenic	1092:1108	arg1	properties					1110:1119	The anti-thrombogenic properties	1088:1119	The anti-thrombogenic properties of the surfaces	1088:1135	The anti-thrombogenic properties of the surfaces were demonstrated by significant decreases in fibrinogen adsorption, Factor XII activation, and platelet adhesion and activation.
31909867	9	34	from	decreases	1170:1178	arg1	adsorption					1194:1203	fibrinogen adsorption	1183:1203	fibrinogen adsorption	1183:1203	The anti-thrombogenic properties of the surfaces were demonstrated by significant decreases in fibrinogen adsorption, Factor XII activation, and platelet adhesion and activation.
31909867	9	34	from	decreases	1170:1178	arg1	activation					1217:1226	Factor XII activation	1206:1226	Factor XII activation	1206:1226	The anti-thrombogenic properties of the surfaces were demonstrated by significant decreases in fibrinogen adsorption, Factor XII activation, and platelet adhesion and activation.
31909867	9	34	from	decreases	1170:1178	arg1	adhesion					1242:1249	platelet adhesion	1233:1249	platelet adhesion	1233:1249	The anti-thrombogenic properties of the surfaces were demonstrated by significant decreases in fibrinogen adsorption, Factor XII activation, and platelet adhesion and activation.
31909867	2	35	used	used	300:303	arg2	implants					273:280	titanium-based implants	258:280	titanium-based implants	258:280	For decades, titanium-based implants have been largely used for different medical applications.
31909867	9	36	theme	Factor	1206:1211	arg1	activation					1217:1226	Factor XII activation	1206:1226	Factor XII activation	1206:1226	The anti-thrombogenic properties of the surfaces were demonstrated by significant decreases in fibrinogen adsorption, Factor XII activation, and platelet adhesion and activation.
31909867	9	37	theme	fibrinogen	1183:1192	arg1	adsorption					1194:1203	fibrinogen adsorption	1183:1203	fibrinogen adsorption	1183:1203	The anti-thrombogenic properties of the surfaces were demonstrated by significant decreases in fibrinogen adsorption, Factor XII activation, and platelet adhesion and activation.
31909867	6	38	theme	surfaces	753:760	arg1	hemocompatibility					728:744	the hemocompatibility	724:744	the hemocompatibility of the surfaces	724:760	To evaluate the hemocompatibility of the surfaces, fibrinogen adsorption, Factor XII activation, and platelet adhesion and activation were analyzed.
31909867	7	39	theme	antibacterial	865:877	arg1	activity					879:886	The antibacterial activity	861:886	The antibacterial activity	861:886	The antibacterial activity was investigated against Gram-negative P. aeruginosa and Gram-positive S. aureus.
31909867	10	40	dep	aeruginosa	1351:1360	arg1	bacteria					1376:1383	bacteria	1376:1383	bacteria	1376:1383	Modifying NT with tanfloc/heparin also reduces the adhesion and proliferation of P. aeruginosa and S. aureus bacteria after 24 hr of incubation, with no biofilm formation.
31909867	11	41	theme	NT	1452:1453	arg1	surfaces					1455:1462	The modified NT surfaces	1439:1462	The modified NT surfaces with tanfloc/heparin polyelectrolyte multilayers	1439:1511	The modified NT surfaces with tanfloc/heparin polyelectrolyte multilayers are a promising biomaterial for use on implant surfaces because of their enhanced blood biocompatibility and antibacterial properties.
31909867	11	41	theme	NT	1452:1453	arg1	biomaterial					1529:1539	a promising biomaterial	1517:1539	a promising biomaterial for use on implant surfaces	1517:1567	The modified NT surfaces with tanfloc/heparin polyelectrolyte multilayers are a promising biomaterial for use on implant surfaces because of their enhanced blood biocompatibility and antibacterial properties.
31909867	11	42	theme	polyelectrolyte	1485:1499	arg1	multilayers					1501:1511	tanfloc/heparin polyelectrolyte multilayers	1469:1511	tanfloc/heparin polyelectrolyte multilayers	1469:1511	The modified NT surfaces with tanfloc/heparin polyelectrolyte multilayers are a promising biomaterial for use on implant surfaces because of their enhanced blood biocompatibility and antibacterial properties.
31909867	3	43	theme	blood	380:384	arg1	coagulation					386:396	blood coagulation	380:396	blood coagulation	380:396	However, titanium can neither suppress blood coagulation, nor prevent bacterial infections.
31909867	5	44	theme	X-ray	634:638	arg1	XPS					668:670	XPS	668:670	XPS	668:670	The surfaces were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and water contact angle measurements.
31909867	5	44	theme	X-ray	634:638	arg1	spectroscopy					654:665	X-ray photoelectron spectroscopy	634:665	X-ray photoelectron spectroscopy (XPS)	634:671	The surfaces were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and water contact angle measurements.
31909867	9	45	theme	surfaces	1128:1135	arg1	properties					1110:1119	The anti-thrombogenic properties	1088:1119	The anti-thrombogenic properties of the surfaces	1088:1135	The anti-thrombogenic properties of the surfaces were demonstrated by significant decreases in fibrinogen adsorption, Factor XII activation, and platelet adhesion and activation.
31909867	4	46	theme	array	527:531	arg1	surfaces					533:540	titania nanotubes array surfaces	509:540	titania nanotubes array surfaces (NT)	509:545	To address these challenges, tanfloc/heparin polyelectrolyte multilayers on titania nanotubes array surfaces (NT) were developed.
31909867	4	46	theme	array	527:531	arg1	NT					543:544	NT	543:544	NT	543:544	To address these challenges, tanfloc/heparin polyelectrolyte multilayers on titania nanotubes array surfaces (NT) were developed.
31909867	11	47	from	biomaterial	1529:1539	arg1	surfaces					1560:1567	implant surfaces	1552:1567	implant surfaces	1552:1567	The modified NT surfaces with tanfloc/heparin polyelectrolyte multilayers are a promising biomaterial for use on implant surfaces because of their enhanced blood biocompatibility and antibacterial properties.
31909867	5	48	theme	photoelectron	640:652	arg1	XPS					668:670	XPS	668:670	XPS	668:670	The surfaces were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and water contact angle measurements.
31909867	5	48	theme	photoelectron	640:652	arg1	spectroscopy					654:665	X-ray photoelectron spectroscopy	634:665	X-ray photoelectron spectroscopy (XPS)	634:671	The surfaces were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and water contact angle measurements.
31909867	1	49	theme	bacterial	171:179	arg1	infection					181:189	bacterial infection	171:189	bacterial infection	171:189	Biomaterial-associated thrombus formation and bacterial infection remain major challenges for blood-contacting devices.
31909867	4	50	theme	nanotubes	517:525	arg1	surfaces					533:540	titania nanotubes array surfaces	509:540	titania nanotubes array surfaces (NT)	509:545	To address these challenges, tanfloc/heparin polyelectrolyte multilayers on titania nanotubes array surfaces (NT) were developed.
31909867	4	50	theme	nanotubes	517:525	arg1	NT					543:544	NT	543:544	NT	543:544	To address these challenges, tanfloc/heparin polyelectrolyte multilayers on titania nanotubes array surfaces (NT) were developed.
31909867	5	51	theme	water	678:682	arg1	measurements					698:709	water contact angle measurements	678:709	water contact angle measurements	678:709	The surfaces were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and water contact angle measurements.
31909867	10	52	theme	aureus	1369:1374	arg1	adhesion					1318:1325	adhesion	1318:1325	adhesion	1318:1325	Modifying NT with tanfloc/heparin also reduces the adhesion and proliferation of P. aeruginosa and S. aureus bacteria after 24 hr of incubation, with no biofilm formation.
31909867	10	52	theme	aureus	1369:1374	arg1	proliferation					1331:1343	proliferation	1331:1343	proliferation	1331:1343	Modifying NT with tanfloc/heparin also reduces the adhesion and proliferation of P. aeruginosa and S. aureus bacteria after 24 hr of incubation, with no biofilm formation.
31909867	2	53	theme	titanium-based	258:271	arg1	implants					273:280	titanium-based implants	258:280	titanium-based implants	258:280	For decades, titanium-based implants have been largely used for different medical applications.
31909867	8	54	theme	fluorescence	1055:1066	arg1	microscopy					1068:1077	fluorescence microscopy	1055:1077	fluorescence microscopy	1055:1077	Bacterial adhesion and morphology, as well as biofilm formation, were analyzed using fluorescence microscopy and SEM.
31909867	6	55	theme	Factor	786:791	arg1	activation					797:806	Factor XII activation	786:806	Factor XII activation	786:806	To evaluate the hemocompatibility of the surfaces, fibrinogen adsorption, Factor XII activation, and platelet adhesion and activation were analyzed.
31909867	0	56	theme	tanfloc/heparin	80:94	arg1	multilayers					112:122	tanfloc/heparin polyelectrolyte multilayers	80:122	tanfloc/heparin polyelectrolyte multilayers	80:122	Enhanced hemocompatibility and antibacterial activity on titania nanotubes with tanfloc/heparin polyelectrolyte multilayers.
31909867	10	57	dep	adhesion	1318:1325	arg1	the					1314:1316	the	1314:1316	the	1314:1316	Modifying NT with tanfloc/heparin also reduces the adhesion and proliferation of P. aeruginosa and S. aureus bacteria after 24 hr of incubation, with no biofilm formation.
31909867	2	58	theme	medical	319:325	arg1	applications					327:338	different medical applications	309:338	different medical applications	309:338	For decades, titanium-based implants have been largely used for different medical applications.
31909867	6	59	theme	platelet	813:820	arg1	adhesion					822:829	platelet adhesion	813:829	platelet adhesion	813:829	To evaluate the hemocompatibility of the surfaces, fibrinogen adsorption, Factor XII activation, and platelet adhesion and activation were analyzed.
31909867	1	60	theme	major	198:202	arg1	challenges					204:213	major challenges	198:213	major challenges for blood-contacting devices	198:242	Biomaterial-associated thrombus formation and bacterial infection remain major challenges for blood-contacting devices.
31909867	9	61	theme	significant	1158:1168	arg1	decreases					1170:1178	significant decreases	1158:1178	significant decreases in fibrinogen adsorption, Factor XII activation, and platelet adhesion and activation	1158:1264	The anti-thrombogenic properties of the surfaces were demonstrated by significant decreases in fibrinogen adsorption, Factor XII activation, and platelet adhesion and activation.
31909867	3	62	theme	bacterial	411:419	arg1	infections					421:430	bacterial infections	411:430	bacterial infections	411:430	However, titanium can neither suppress blood coagulation, nor prevent bacterial infections.
31909867	2	63	theme	different	309:317	arg1	applications					327:338	different medical applications	309:338	different medical applications	309:338	For decades, titanium-based implants have been largely used for different medical applications.
31909867	10	64	theme	incubation	1400:1409	arg1	24 hr					1391:1395	24 hr	1391:1395	24 hr of incubation	1391:1409	Modifying NT with tanfloc/heparin also reduces the adhesion and proliferation of P. aeruginosa and S. aureus bacteria after 24 hr of incubation, with no biofilm formation.
32924916	1	0	theme	antimicrobial	77:89	arg1	potential					91:99	antimicrobial potential	77:99	antimicrobial potential	77:99	nov., isolated from pine grove soil, exhibiting antimicrobial potential.
32924916	8	1	theme	phylogenetic	1355:1366	arg1	properties					1399:1408	The phylogenetic, chemotaxonomic and phenotypic properties	1351:1408	The phylogenetic, chemotaxonomic and phenotypic properties	1351:1408	The phylogenetic, chemotaxonomic and phenotypic properties enabled distinction of MMS16-CNU292T from related species, and thus the isolate should be recognized as a new species of the genus Kitasatospora, for which the name Kitasatospora acidiphila sp.
32924916	3	2	theme	KCTC	491:494	arg1	9699T					496:500	Kitasatospora azatica KCTC 9699T	469:500	Kitasatospora azatica KCTC 9699T (98.75 %)	469:510	On the basis of 16S rRNA gene sequence similarity, the strain formed a novel evolutionary lineage within Kitasatospora and showed highest similarities to Kitasatospora azatica KCTC 9699T (98.75 %), Kitasatospora kifunensis IFO 15206T (98.74 %), Kitasatospora purpeofusca NRRL B-1817T (98.61 %) and Kitasatospora nipponensis HKI 0315T (98.42 %), respectively.
32924916	3	2	theme	KCTC	491:494	arg1	%					509:509	98.75 %	503:509	98.75 %	503:509	On the basis of 16S rRNA gene sequence similarity, the strain formed a novel evolutionary lineage within Kitasatospora and showed highest similarities to Kitasatospora azatica KCTC 9699T (98.75 %), Kitasatospora kifunensis IFO 15206T (98.74 %), Kitasatospora purpeofusca NRRL B-1817T (98.61 %) and Kitasatospora nipponensis HKI 0315T (98.42 %), respectively.
32924916	3	2	theme	KCTC	491:494	arg1	15206T					542:547	15206T	542:547	15206T	542:547	On the basis of 16S rRNA gene sequence similarity, the strain formed a novel evolutionary lineage within Kitasatospora and showed highest similarities to Kitasatospora azatica KCTC 9699T (98.75 %), Kitasatospora kifunensis IFO 15206T (98.74 %), Kitasatospora purpeofusca NRRL B-1817T (98.61 %) and Kitasatospora nipponensis HKI 0315T (98.42 %), respectively.
32924916	7	3	theme	acidic	1332:1337	arg1	conditions					1339:1348	acidic conditions	1332:1348	acidic conditions	1332:1348	The strain exhibited antibacterial activity against a number of bacterial strains, and the activity was generally greater when grown in acidic conditions.
32924916	2	4	theme	polyphasic	104:113	arg1	study					115:119	A polyphasic study	102:119	A polyphasic study	102:119	A polyphasic study was carried out to establish the taxonomic position of an acidophilic isolate designated MMS16-CNU292T (=JCM 32302T) from pine grove soil, and provisionally assigned to the genus Kitasatospora.
32924916	8	5	theme	related	1452:1458	arg1	species					1460:1466	related species	1452:1466	related species	1452:1466	The phylogenetic, chemotaxonomic and phenotypic properties enabled distinction of MMS16-CNU292T from related species, and thus the isolate should be recognized as a new species of the genus Kitasatospora, for which the name Kitasatospora acidiphila sp.
32924916	3	6	theme	16S	331:333	arg1	similarity					354:363	16S rRNA gene sequence similarity	331:363	16S rRNA gene sequence similarity	331:363	On the basis of 16S rRNA gene sequence similarity, the strain formed a novel evolutionary lineage within Kitasatospora and showed highest similarities to Kitasatospora azatica KCTC 9699T (98.75 %), Kitasatospora kifunensis IFO 15206T (98.74 %), Kitasatospora purpeofusca NRRL B-1817T (98.61 %) and Kitasatospora nipponensis HKI 0315T (98.42 %), respectively.
32924916	4	7	from	amount	766:771	arg1	peptidoglycan					818:830	the cell-wall peptidoglycan	804:830	the cell-wall peptidoglycan	804:830	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	3	8	dep	similarity	354:363	arg1	the					318:320	the	318:320	the	318:320	On the basis of 16S rRNA gene sequence similarity, the strain formed a novel evolutionary lineage within Kitasatospora and showed highest similarities to Kitasatospora azatica KCTC 9699T (98.75 %), Kitasatospora kifunensis IFO 15206T (98.74 %), Kitasatospora purpeofusca NRRL B-1817T (98.61 %) and Kitasatospora nipponensis HKI 0315T (98.42 %), respectively.
32924916	3	8	dep	similarity	354:363	arg1	basis					322:326	basis	322:326	basis	322:326	On the basis of 16S rRNA gene sequence similarity, the strain formed a novel evolutionary lineage within Kitasatospora and showed highest similarities to Kitasatospora azatica KCTC 9699T (98.75 %), Kitasatospora kifunensis IFO 15206T (98.74 %), Kitasatospora purpeofusca NRRL B-1817T (98.61 %) and Kitasatospora nipponensis HKI 0315T (98.42 %), respectively.
32924916	1	9	theme	pine	49:52	arg1	soil					60:63	pine grove soil	49:63	pine grove soil	49:63	nov., isolated from pine grove soil, exhibiting antimicrobial potential.
32924916	5	10	from	glucose	885:891	arg1	rich					866:869	rich	866:869	rich	866:869	The whole-cell hydrolysates were rich in galactose, glucose and mannose, and the polar lipids mainly consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and phosphatidylinositol mannosides.
32924916	5	10	from	glucose	885:891	arg1	hydrolysates					848:859	The whole-cell hydrolysates	833:859	The whole-cell hydrolysates	833:859	The whole-cell hydrolysates were rich in galactose, glucose and mannose, and the polar lipids mainly consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and phosphatidylinositol mannosides.
32924916	3	11	theme	rRNA	335:338	arg1	similarity					354:363	16S rRNA gene sequence similarity	331:363	16S rRNA gene sequence similarity	331:363	On the basis of 16S rRNA gene sequence similarity, the strain formed a novel evolutionary lineage within Kitasatospora and showed highest similarities to Kitasatospora azatica KCTC 9699T (98.75 %), Kitasatospora kifunensis IFO 15206T (98.74 %), Kitasatospora purpeofusca NRRL B-1817T (98.61 %) and Kitasatospora nipponensis HKI 0315T (98.42 %), respectively.
32924916	5	12	theme	phosphatidylinositol	1044:1063	arg1	mannosides					1065:1074	phosphatidylinositol mannosides	1044:1074	phosphatidylinositol mannosides	1044:1074	The whole-cell hydrolysates were rich in galactose, glucose and mannose, and the polar lipids mainly consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and phosphatidylinositol mannosides.
32924916	4	13	theme	meso-diaminopimelic	776:794	arg1	acid					796:799	meso-diaminopimelic acid	776:799	meso-diaminopimelic acid in the cell-wall peptidoglycan	776:830	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	1	14	theme	grove	54:58	arg1	soil					60:63	pine grove soil	49:63	pine grove soil	49:63	nov., isolated from pine grove soil, exhibiting antimicrobial potential.
32924916	1	15	attach	isolated	35:42	arg1	soil					60:63	pine grove soil	49:63	pine grove soil	49:63	nov., isolated from pine grove soil, exhibiting antimicrobial potential.
32924916	1	15	attach	isolated	35:42	arg2	nov.					29:32	nov.	29:32	nov.	29:32	nov., isolated from pine grove soil, exhibiting antimicrobial potential.
32924916	6	16	theme	71.5 mol	1185:1192	arg1	content					1173:1179	the DNA G+C content	1161:1179	the DNA G+C content	1161:1179	The major fatty acids were anteiso-C15 : 1-A, anteiso-C15 : 0, and iso-C15 : 0, and the DNA G+C content was 71.5 mol%.
32924916	6	16	theme	71.5 mol	1185:1192	arg1	%					1193:1193	71.5 mol%	1185:1193	71.5 mol%	1185:1193	The major fatty acids were anteiso-C15 : 1-A, anteiso-C15 : 0, and iso-C15 : 0, and the DNA G+C content was 71.5 mol%.
32924916	8	17	theme	phenotypic	1388:1397	arg1	properties					1399:1408	The phylogenetic, chemotaxonomic and phenotypic properties	1351:1408	The phylogenetic, chemotaxonomic and phenotypic properties	1351:1408	The phylogenetic, chemotaxonomic and phenotypic properties enabled distinction of MMS16-CNU292T from related species, and thus the isolate should be recognized as a new species of the genus Kitasatospora, for which the name Kitasatospora acidiphila sp.
32924916	8	18	theme	acidiphila	1589:1598	arg1	sp					1600:1601	the name Kitasatospora acidiphila sp	1566:1601	the name Kitasatospora acidiphila sp	1566:1601	The phylogenetic, chemotaxonomic and phenotypic properties enabled distinction of MMS16-CNU292T from related species, and thus the isolate should be recognized as a new species of the genus Kitasatospora, for which the name Kitasatospora acidiphila sp.
32924916	6	19	dep	anteiso-C15 	1104:1115	arg1	 1-A					1117:1120	 1-A	1117:1120	 1-A	1117:1120	The major fatty acids were anteiso-C15 : 1-A, anteiso-C15 : 0, and iso-C15 : 0, and the DNA G+C content was 71.5 mol%.
32924916	6	19	dep	anteiso-C15 	1104:1115	arg1	 0					1153:1154	 0	1153:1154	 0	1153:1154	The major fatty acids were anteiso-C15 : 1-A, anteiso-C15 : 0, and iso-C15 : 0, and the DNA G+C content was 71.5 mol%.
32924916	6	19	dep	anteiso-C15 	1104:1115	arg1	 0					1136:1137	 0	1136:1137	 0	1136:1137	The major fatty acids were anteiso-C15 : 1-A, anteiso-C15 : 0, and iso-C15 : 0, and the DNA G+C content was 71.5 mol%.
32924916	6	19	dep	anteiso-C15 	1104:1115	arg1	iso-C15 					1144:1151	iso-C15 	1144:1151	iso-C15 	1144:1151	The major fatty acids were anteiso-C15 : 1-A, anteiso-C15 : 0, and iso-C15 : 0, and the DNA G+C content was 71.5 mol%.
32924916	6	19	dep	anteiso-C15 	1104:1115	arg1	anteiso-C15 					1123:1134	anteiso-C15 	1123:1134	anteiso-C15 	1123:1134	The major fatty acids were anteiso-C15 : 1-A, anteiso-C15 : 0, and iso-C15 : 0, and the DNA G+C content was 71.5 mol%.
32924916	0	20	theme	acidiphila	14:23	arg1	sp					25:26	Kitasatospora acidiphila sp	0:26	Kitasatospora acidiphila sp.	0:27	Kitasatospora acidiphila sp.
32924916	4	21	theme	major	760:764	arg1	MK-9					718:721	MK-9	718:721	MK-9	718:721	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	4	21	theme	major	760:764	arg1	MK-9					705:708	MK-9	705:708	MK-9	705:708	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	4	21	theme	major	760:764	arg1	amount					766:771	a major amount	758:771	a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan	758:830	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	4	21	theme	major	760:764	arg1	acid					796:799	meso-diaminopimelic acid	776:799	meso-diaminopimelic acid in the cell-wall peptidoglycan	776:830	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	2	22	theme	genus	294:298	arg1	Kitasatospora					300:312	the genus Kitasatospora	290:312	the genus Kitasatospora	290:312	A polyphasic study was carried out to establish the taxonomic position of an acidophilic isolate designated MMS16-CNU292T (=JCM 32302T) from pine grove soil, and provisionally assigned to the genus Kitasatospora.
32924916	8	23	theme	genus	1535:1539	arg1	Kitasatospora					1541:1553	the genus Kitasatospora	1531:1553	the genus Kitasatospora	1531:1553	The phylogenetic, chemotaxonomic and phenotypic properties enabled distinction of MMS16-CNU292T from related species, and thus the isolate should be recognized as a new species of the genus Kitasatospora, for which the name Kitasatospora acidiphila sp.
32924916	3	24	theme	novel	386:390	arg1	lineage					405:411	a novel evolutionary lineage	384:411	a novel evolutionary lineage	384:411	On the basis of 16S rRNA gene sequence similarity, the strain formed a novel evolutionary lineage within Kitasatospora and showed highest similarities to Kitasatospora azatica KCTC 9699T (98.75 %), Kitasatospora kifunensis IFO 15206T (98.74 %), Kitasatospora purpeofusca NRRL B-1817T (98.61 %) and Kitasatospora nipponensis HKI 0315T (98.42 %), respectively.
32924916	0	25	theme	Kitasatospora	0:12	arg1	sp					25:26	Kitasatospora acidiphila sp	0:26	Kitasatospora acidiphila sp.	0:27	Kitasatospora acidiphila sp.
32924916	4	26	contain	possessed	695:703	arg2	menaquinones					740:751	the major menaquinones	730:751	the major menaquinones	730:751	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	4	26	contain	possessed	695:703	arg2	amount					766:771	a major amount	758:771	a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan	758:830	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	4	26	contain	possessed	695:703	arg2	acid					796:799	meso-diaminopimelic acid	776:799	meso-diaminopimelic acid in the cell-wall peptidoglycan	776:830	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	4	26	contain	possessed	695:703	arg2	MK-9					705:708	MK-9	705:708	MK-9	705:708	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	4	26	contain	possessed	695:703	arg2	MK-9					718:721	MK-9	718:721	MK-9	718:721	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	4	26	contain	possessed	695:703	arg2	H6					710:711	H6	710:711	H6	710:711	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	4	26	contain	possessed	695:703	arg1	MMS16-CNU292T					681:693	Strain MMS16-CNU292T	674:693	Strain MMS16-CNU292T	674:693	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	4	26	contain	possessed	695:703	arg2	H8					723:724	H8	723:724	H8	723:724	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	6	27	theme	G+C	1169:1171	arg1	content					1173:1179	the DNA G+C content	1161:1179	the DNA G+C content	1161:1179	The major fatty acids were anteiso-C15 : 1-A, anteiso-C15 : 0, and iso-C15 : 0, and the DNA G+C content was 71.5 mol%.
32924916	6	27	theme	G+C	1169:1171	arg1	%					1193:1193	71.5 mol%	1185:1193	71.5 mol%	1185:1193	The major fatty acids were anteiso-C15 : 1-A, anteiso-C15 : 0, and iso-C15 : 0, and the DNA G+C content was 71.5 mol%.
32924916	3	28	theme	NRRL	586:589	arg1	%					606:606	98.61 %	600:606	98.61 %	600:606	On the basis of 16S rRNA gene sequence similarity, the strain formed a novel evolutionary lineage within Kitasatospora and showed highest similarities to Kitasatospora azatica KCTC 9699T (98.75 %), Kitasatospora kifunensis IFO 15206T (98.74 %), Kitasatospora purpeofusca NRRL B-1817T (98.61 %) and Kitasatospora nipponensis HKI 0315T (98.42 %), respectively.
32924916	3	28	theme	NRRL	586:589	arg1	B-1817T					591:597	NRRL B-1817T	586:597	Kitasatospora purpeofusca NRRL B-1817T (98.61 %)	560:607	On the basis of 16S rRNA gene sequence similarity, the strain formed a novel evolutionary lineage within Kitasatospora and showed highest similarities to Kitasatospora azatica KCTC 9699T (98.75 %), Kitasatospora kifunensis IFO 15206T (98.74 %), Kitasatospora purpeofusca NRRL B-1817T (98.61 %) and Kitasatospora nipponensis HKI 0315T (98.42 %), respectively.
32924916	3	29	theme	evolutionary	392:403	arg1	lineage					405:411	a novel evolutionary lineage	384:411	a novel evolutionary lineage	384:411	On the basis of 16S rRNA gene sequence similarity, the strain formed a novel evolutionary lineage within Kitasatospora and showed highest similarities to Kitasatospora azatica KCTC 9699T (98.75 %), Kitasatospora kifunensis IFO 15206T (98.74 %), Kitasatospora purpeofusca NRRL B-1817T (98.61 %) and Kitasatospora nipponensis HKI 0315T (98.42 %), respectively.
32924916	3	30	theme	gene	340:343	arg1	similarity					354:363	16S rRNA gene sequence similarity	331:363	16S rRNA gene sequence similarity	331:363	On the basis of 16S rRNA gene sequence similarity, the strain formed a novel evolutionary lineage within Kitasatospora and showed highest similarities to Kitasatospora azatica KCTC 9699T (98.75 %), Kitasatospora kifunensis IFO 15206T (98.74 %), Kitasatospora purpeofusca NRRL B-1817T (98.61 %) and Kitasatospora nipponensis HKI 0315T (98.42 %), respectively.
32924916	4	31	theme	Strain	674:679	arg1	MMS16-CNU292T					681:693	Strain MMS16-CNU292T	674:693	Strain MMS16-CNU292T	674:693	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	9	32	theme	type	1610:1613	arg1	nov					1604:1606	nov	1604:1606	nov	1604:1606	nov. (type strain=MMS16-CNU292T=KCTC 49011T=JCM 32302T) is proposed.
32924916	9	32	theme	type	1610:1613	arg1	32302T					1652:1657	type strain=MMS16-CNU292T=KCTC 49011T=JCM 32302T	1610:1657	type strain=MMS16-CNU292T=KCTC 49011T=JCM 32302T	1610:1657	nov. (type strain=MMS16-CNU292T=KCTC 49011T=JCM 32302T) is proposed.
32924916	3	33	dep	Kitasatospora	560:572	arg1	%					606:606	98.61 %	600:606	98.61 %	600:606	On the basis of 16S rRNA gene sequence similarity, the strain formed a novel evolutionary lineage within Kitasatospora and showed highest similarities to Kitasatospora azatica KCTC 9699T (98.75 %), Kitasatospora kifunensis IFO 15206T (98.74 %), Kitasatospora purpeofusca NRRL B-1817T (98.61 %) and Kitasatospora nipponensis HKI 0315T (98.42 %), respectively.
32924916	3	33	dep	Kitasatospora	560:572	arg1	purpeofusca					574:584	Kitasatospora purpeofusca NRRL B-1817T (98.61 %)	560:607	Kitasatospora purpeofusca NRRL B-1817T (98.61 %)	560:607	On the basis of 16S rRNA gene sequence similarity, the strain formed a novel evolutionary lineage within Kitasatospora and showed highest similarities to Kitasatospora azatica KCTC 9699T (98.75 %), Kitasatospora kifunensis IFO 15206T (98.74 %), Kitasatospora purpeofusca NRRL B-1817T (98.61 %) and Kitasatospora nipponensis HKI 0315T (98.42 %), respectively.
32924916	3	33	dep	Kitasatospora	560:572	arg1	B-1817T					591:597	NRRL B-1817T	586:597	Kitasatospora purpeofusca NRRL B-1817T (98.61 %)	560:607	On the basis of 16S rRNA gene sequence similarity, the strain formed a novel evolutionary lineage within Kitasatospora and showed highest similarities to Kitasatospora azatica KCTC 9699T (98.75 %), Kitasatospora kifunensis IFO 15206T (98.74 %), Kitasatospora purpeofusca NRRL B-1817T (98.61 %) and Kitasatospora nipponensis HKI 0315T (98.42 %), respectively.
32924916	3	34	theme	sequence	345:352	arg1	similarity					354:363	16S rRNA gene sequence similarity	331:363	16S rRNA gene sequence similarity	331:363	On the basis of 16S rRNA gene sequence similarity, the strain formed a novel evolutionary lineage within Kitasatospora and showed highest similarities to Kitasatospora azatica KCTC 9699T (98.75 %), Kitasatospora kifunensis IFO 15206T (98.74 %), Kitasatospora purpeofusca NRRL B-1817T (98.61 %) and Kitasatospora nipponensis HKI 0315T (98.42 %), respectively.
32924916	9	35	theme	strain=MMS16-CNU292T=KCTC	1615:1639	arg1	nov					1604:1606	nov	1604:1606	nov	1604:1606	nov. (type strain=MMS16-CNU292T=KCTC 49011T=JCM 32302T) is proposed.
32924916	9	35	theme	strain=MMS16-CNU292T=KCTC	1615:1639	arg1	32302T					1652:1657	type strain=MMS16-CNU292T=KCTC 49011T=JCM 32302T	1610:1657	type strain=MMS16-CNU292T=KCTC 49011T=JCM 32302T	1610:1657	nov. (type strain=MMS16-CNU292T=KCTC 49011T=JCM 32302T) is proposed.
32924916	8	36	theme	chemotaxonomic	1369:1382	arg1	properties					1399:1408	The phylogenetic, chemotaxonomic and phenotypic properties	1351:1408	The phylogenetic, chemotaxonomic and phenotypic properties	1351:1408	The phylogenetic, chemotaxonomic and phenotypic properties enabled distinction of MMS16-CNU292T from related species, and thus the isolate should be recognized as a new species of the genus Kitasatospora, for which the name Kitasatospora acidiphila sp.
32924916	5	37	from	lipids	920:925	arg1	rich					866:869	rich	866:869	rich	866:869	The whole-cell hydrolysates were rich in galactose, glucose and mannose, and the polar lipids mainly consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and phosphatidylinositol mannosides.
32924916	5	37	from	lipids	920:925	arg1	hydrolysates					848:859	The whole-cell hydrolysates	833:859	The whole-cell hydrolysates	833:859	The whole-cell hydrolysates were rich in galactose, glucose and mannose, and the polar lipids mainly consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and phosphatidylinositol mannosides.
32924916	3	38	theme	highest	445:451	arg1	similarities					453:464	highest similarities	445:464	highest similarities to Kitasatospora azatica KCTC 9699T (98.75 %), Kitasatospora kifunensis IFO 15206T (98.74 %), Kitasatospora purpeofusca NRRL B-1817T (98.61 %) and Kitasatospora nipponensis HKI 0315T (98.42 %), respectively	445:671	On the basis of 16S rRNA gene sequence similarity, the strain formed a novel evolutionary lineage within Kitasatospora and showed highest similarities to Kitasatospora azatica KCTC 9699T (98.75 %), Kitasatospora kifunensis IFO 15206T (98.74 %), Kitasatospora purpeofusca NRRL B-1817T (98.61 %) and Kitasatospora nipponensis HKI 0315T (98.42 %), respectively.
32924916	9	39	theme	49011T=JCM	1641:1650	arg1	nov					1604:1606	nov	1604:1606	nov	1604:1606	nov. (type strain=MMS16-CNU292T=KCTC 49011T=JCM 32302T) is proposed.
32924916	9	39	theme	49011T=JCM	1641:1650	arg1	32302T					1652:1657	type strain=MMS16-CNU292T=KCTC 49011T=JCM 32302T	1610:1657	type strain=MMS16-CNU292T=KCTC 49011T=JCM 32302T	1610:1657	nov. (type strain=MMS16-CNU292T=KCTC 49011T=JCM 32302T) is proposed.
32924916	6	40	theme	DNA	1165:1167	arg1	content					1173:1179	the DNA G+C content	1161:1179	the DNA G+C content	1161:1179	The major fatty acids were anteiso-C15 : 1-A, anteiso-C15 : 0, and iso-C15 : 0, and the DNA G+C content was 71.5 mol%.
32924916	6	40	theme	DNA	1165:1167	arg1	%					1193:1193	71.5 mol%	1185:1193	71.5 mol%	1185:1193	The major fatty acids were anteiso-C15 : 1-A, anteiso-C15 : 0, and iso-C15 : 0, and the DNA G+C content was 71.5 mol%.
32924916	5	41	from	rich	866:869	arg1	glucose					885:891	glucose	885:891	glucose	885:891	The whole-cell hydrolysates were rich in galactose, glucose and mannose, and the polar lipids mainly consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and phosphatidylinositol mannosides.
32924916	5	41	from	rich	866:869	arg1	galactose					874:882	galactose	874:882	galactose	874:882	The whole-cell hydrolysates were rich in galactose, glucose and mannose, and the polar lipids mainly consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and phosphatidylinositol mannosides.
32924916	5	41	from	rich	866:869	arg1	lipids					920:925	the polar lipids	910:925	the polar lipids mainly consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and phosphatidylinositol mannosides	910:1074	The whole-cell hydrolysates were rich in galactose, glucose and mannose, and the polar lipids mainly consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and phosphatidylinositol mannosides.
32924916	2	42	theme	taxonomic	154:162	arg1	position					164:171	the taxonomic position	150:171	the taxonomic position of an acidophilic isolate designated MMS16-CNU292T (=JCM 32302T) from pine grove soil, and provisionally assigned to the genus Kitasatospora	150:312	A polyphasic study was carried out to establish the taxonomic position of an acidophilic isolate designated MMS16-CNU292T (=JCM 32302T) from pine grove soil, and provisionally assigned to the genus Kitasatospora.
32924916	4	43	from	menaquinones	740:751	arg1	peptidoglycan					818:830	the cell-wall peptidoglycan	804:830	the cell-wall peptidoglycan	804:830	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	7	44	theme	antibacterial	1217:1229	arg1	activity					1231:1238	antibacterial activity	1217:1238	antibacterial activity against a number of bacterial strains	1217:1276	The strain exhibited antibacterial activity against a number of bacterial strains, and the activity was generally greater when grown in acidic conditions.
32924916	4	45	theme	acid	796:799	arg1	MK-9					718:721	MK-9	718:721	MK-9	718:721	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	4	45	theme	acid	796:799	arg1	acid					796:799	meso-diaminopimelic acid	776:799	meso-diaminopimelic acid in the cell-wall peptidoglycan	776:830	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	4	45	theme	acid	796:799	arg1	MK-9					705:708	MK-9	705:708	MK-9	705:708	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	4	45	theme	acid	796:799	arg1	amount					766:771	a major amount	758:771	a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan	758:830	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	4	45	theme	acid	796:799	arg1	menaquinones					740:751	the major menaquinones	730:751	the major menaquinones	730:751	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	8	46	theme	MMS16-CNU292T	1433:1445	arg1	distinction					1418:1428	distinction	1418:1428	distinction of MMS16-CNU292T from related species	1418:1466	The phylogenetic, chemotaxonomic and phenotypic properties enabled distinction of MMS16-CNU292T from related species, and thus the isolate should be recognized as a new species of the genus Kitasatospora, for which the name Kitasatospora acidiphila sp.
32924916	3	47	theme	Kitasatospora	469:481	arg1	9699T					496:500	Kitasatospora azatica KCTC 9699T	469:500	Kitasatospora azatica KCTC 9699T (98.75 %)	469:510	On the basis of 16S rRNA gene sequence similarity, the strain formed a novel evolutionary lineage within Kitasatospora and showed highest similarities to Kitasatospora azatica KCTC 9699T (98.75 %), Kitasatospora kifunensis IFO 15206T (98.74 %), Kitasatospora purpeofusca NRRL B-1817T (98.61 %) and Kitasatospora nipponensis HKI 0315T (98.42 %), respectively.
32924916	3	47	theme	Kitasatospora	469:481	arg1	%					509:509	98.75 %	503:509	98.75 %	503:509	On the basis of 16S rRNA gene sequence similarity, the strain formed a novel evolutionary lineage within Kitasatospora and showed highest similarities to Kitasatospora azatica KCTC 9699T (98.75 %), Kitasatospora kifunensis IFO 15206T (98.74 %), Kitasatospora purpeofusca NRRL B-1817T (98.61 %) and Kitasatospora nipponensis HKI 0315T (98.42 %), respectively.
32924916	3	47	theme	Kitasatospora	469:481	arg1	15206T					542:547	15206T	542:547	15206T	542:547	On the basis of 16S rRNA gene sequence similarity, the strain formed a novel evolutionary lineage within Kitasatospora and showed highest similarities to Kitasatospora azatica KCTC 9699T (98.75 %), Kitasatospora kifunensis IFO 15206T (98.74 %), Kitasatospora purpeofusca NRRL B-1817T (98.61 %) and Kitasatospora nipponensis HKI 0315T (98.42 %), respectively.
32924916	8	48	theme	name	1570:1573	arg1	sp					1600:1601	the name Kitasatospora acidiphila sp	1566:1601	the name Kitasatospora acidiphila sp	1566:1601	The phylogenetic, chemotaxonomic and phenotypic properties enabled distinction of MMS16-CNU292T from related species, and thus the isolate should be recognized as a new species of the genus Kitasatospora, for which the name Kitasatospora acidiphila sp.
32924916	4	49	from	peptidoglycan	818:830	arg1	MK-9					718:721	MK-9	718:721	MK-9	718:721	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	4	49	from	peptidoglycan	818:830	arg1	acid					796:799	meso-diaminopimelic acid	776:799	meso-diaminopimelic acid in the cell-wall peptidoglycan	776:830	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	4	49	from	peptidoglycan	818:830	arg1	MK-9					705:708	MK-9	705:708	MK-9	705:708	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	4	49	from	peptidoglycan	818:830	arg1	amount					766:771	a major amount	758:771	a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan	758:830	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	4	49	from	peptidoglycan	818:830	arg1	menaquinones					740:751	the major menaquinones	730:751	the major menaquinones	730:751	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	4	50	theme	major	734:738	arg1	MK-9					718:721	MK-9	718:721	MK-9	718:721	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	4	50	theme	major	734:738	arg1	MK-9					705:708	MK-9	705:708	MK-9	705:708	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	4	50	theme	major	734:738	arg1	menaquinones					740:751	the major menaquinones	730:751	the major menaquinones	730:751	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	7	51	theme	bacterial	1260:1268	arg1	strains					1270:1276	bacterial strains	1260:1276	bacterial strains	1260:1276	The strain exhibited antibacterial activity against a number of bacterial strains, and the activity was generally greater when grown in acidic conditions.
32924916	3	52	theme	azatica	483:489	arg1	9699T					496:500	Kitasatospora azatica KCTC 9699T	469:500	Kitasatospora azatica KCTC 9699T (98.75 %)	469:510	On the basis of 16S rRNA gene sequence similarity, the strain formed a novel evolutionary lineage within Kitasatospora and showed highest similarities to Kitasatospora azatica KCTC 9699T (98.75 %), Kitasatospora kifunensis IFO 15206T (98.74 %), Kitasatospora purpeofusca NRRL B-1817T (98.61 %) and Kitasatospora nipponensis HKI 0315T (98.42 %), respectively.
32924916	3	52	theme	azatica	483:489	arg1	%					509:509	98.75 %	503:509	98.75 %	503:509	On the basis of 16S rRNA gene sequence similarity, the strain formed a novel evolutionary lineage within Kitasatospora and showed highest similarities to Kitasatospora azatica KCTC 9699T (98.75 %), Kitasatospora kifunensis IFO 15206T (98.74 %), Kitasatospora purpeofusca NRRL B-1817T (98.61 %) and Kitasatospora nipponensis HKI 0315T (98.42 %), respectively.
32924916	3	52	theme	azatica	483:489	arg1	15206T					542:547	15206T	542:547	15206T	542:547	On the basis of 16S rRNA gene sequence similarity, the strain formed a novel evolutionary lineage within Kitasatospora and showed highest similarities to Kitasatospora azatica KCTC 9699T (98.75 %), Kitasatospora kifunensis IFO 15206T (98.74 %), Kitasatospora purpeofusca NRRL B-1817T (98.61 %) and Kitasatospora nipponensis HKI 0315T (98.42 %), respectively.
32924916	8	53	theme	Kitasatospora	1575:1587	arg1	sp					1600:1601	the name Kitasatospora acidiphila sp	1566:1601	the name Kitasatospora acidiphila sp	1566:1601	The phylogenetic, chemotaxonomic and phenotypic properties enabled distinction of MMS16-CNU292T from related species, and thus the isolate should be recognized as a new species of the genus Kitasatospora, for which the name Kitasatospora acidiphila sp.
32924916	8	54	theme	new	1516:1518	arg1	isolate					1482:1488	the isolate	1478:1488	the isolate	1478:1488	The phylogenetic, chemotaxonomic and phenotypic properties enabled distinction of MMS16-CNU292T from related species, and thus the isolate should be recognized as a new species of the genus Kitasatospora, for which the name Kitasatospora acidiphila sp.
32924916	8	54	theme	new	1516:1518	arg1	species					1520:1526	a new species	1514:1526	a new species	1514:1526	The phylogenetic, chemotaxonomic and phenotypic properties enabled distinction of MMS16-CNU292T from related species, and thus the isolate should be recognized as a new species of the genus Kitasatospora, for which the name Kitasatospora acidiphila sp.
32924916	2	55	theme	isolate	191:197	arg1	position					164:171	the taxonomic position	150:171	the taxonomic position of an acidophilic isolate designated MMS16-CNU292T (=JCM 32302T) from pine grove soil, and provisionally assigned to the genus Kitasatospora	150:312	A polyphasic study was carried out to establish the taxonomic position of an acidophilic isolate designated MMS16-CNU292T (=JCM 32302T) from pine grove soil, and provisionally assigned to the genus Kitasatospora.
32924916	2	56	theme	grove	248:252	arg1	soil					254:257	pine grove soil	243:257	pine grove soil	243:257	A polyphasic study was carried out to establish the taxonomic position of an acidophilic isolate designated MMS16-CNU292T (=JCM 32302T) from pine grove soil, and provisionally assigned to the genus Kitasatospora.
32924916	5	57	theme	whole-cell	837:846	arg1	rich					866:869	rich	866:869	rich	866:869	The whole-cell hydrolysates were rich in galactose, glucose and mannose, and the polar lipids mainly consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and phosphatidylinositol mannosides.
32924916	5	57	theme	whole-cell	837:846	arg1	hydrolysates					848:859	The whole-cell hydrolysates	833:859	The whole-cell hydrolysates	833:859	The whole-cell hydrolysates were rich in galactose, glucose and mannose, and the polar lipids mainly consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and phosphatidylinositol mannosides.
32924916	2	58	from	soil	254:257	arg1	MMS16-CNU292T					210:222	MMS16-CNU292T	210:222	MMS16-CNU292T (=JCM 32302T) from pine grove soil	210:257	A polyphasic study was carried out to establish the taxonomic position of an acidophilic isolate designated MMS16-CNU292T (=JCM 32302T) from pine grove soil, and provisionally assigned to the genus Kitasatospora.
32924916	2	58	from	soil	254:257	arg1	32302T					230:235	=JCM 32302T	225:235	=JCM 32302T	225:235	A polyphasic study was carried out to establish the taxonomic position of an acidophilic isolate designated MMS16-CNU292T (=JCM 32302T) from pine grove soil, and provisionally assigned to the genus Kitasatospora.
32924916	4	59	theme	cell-wall	808:816	arg1	peptidoglycan					818:830	the cell-wall peptidoglycan	804:830	the cell-wall peptidoglycan	804:830	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	2	60	theme	acidophilic	179:189	arg1	isolate					191:197	an acidophilic isolate	176:197	an acidophilic isolate designated MMS16-CNU292T (=JCM 32302T) from pine grove soil, and provisionally assigned to the genus Kitasatospora	176:312	A polyphasic study was carried out to establish the taxonomic position of an acidophilic isolate designated MMS16-CNU292T (=JCM 32302T) from pine grove soil, and provisionally assigned to the genus Kitasatospora.
32924916	2	61	theme	pine	243:246	arg1	soil					254:257	pine grove soil	243:257	pine grove soil	243:257	A polyphasic study was carried out to establish the taxonomic position of an acidophilic isolate designated MMS16-CNU292T (=JCM 32302T) from pine grove soil, and provisionally assigned to the genus Kitasatospora.
32924916	6	62	theme	major	1081:1085	arg1	anteiso-C15 					1104:1115	anteiso-C15 	1104:1115	anteiso-C15 	1104:1115	The major fatty acids were anteiso-C15 : 1-A, anteiso-C15 : 0, and iso-C15 : 0, and the DNA G+C content was 71.5 mol%.
32924916	6	62	theme	major	1081:1085	arg1	acids					1093:1097	The major fatty acids	1077:1097	The major fatty acids	1077:1097	The major fatty acids were anteiso-C15 : 1-A, anteiso-C15 : 0, and iso-C15 : 0, and the DNA G+C content was 71.5 mol%.
32924916	6	63	theme	fatty	1087:1091	arg1	anteiso-C15 					1104:1115	anteiso-C15 	1104:1115	anteiso-C15 	1104:1115	The major fatty acids were anteiso-C15 : 1-A, anteiso-C15 : 0, and iso-C15 : 0, and the DNA G+C content was 71.5 mol%.
32924916	6	63	theme	fatty	1087:1091	arg1	acids					1093:1097	The major fatty acids	1077:1097	The major fatty acids	1077:1097	The major fatty acids were anteiso-C15 : 1-A, anteiso-C15 : 0, and iso-C15 : 0, and the DNA G+C content was 71.5 mol%.
32924916	7	64	theme	strains	1270:1276	arg1	number					1250:1255	a number	1248:1255	a number of bacterial strains	1248:1276	The strain exhibited antibacterial activity against a number of bacterial strains, and the activity was generally greater when grown in acidic conditions.
32924916	5	65	from	galactose	874:882	arg1	rich					866:869	rich	866:869	rich	866:869	The whole-cell hydrolysates were rich in galactose, glucose and mannose, and the polar lipids mainly consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and phosphatidylinositol mannosides.
32924916	5	65	from	galactose	874:882	arg1	hydrolysates					848:859	The whole-cell hydrolysates	833:859	The whole-cell hydrolysates	833:859	The whole-cell hydrolysates were rich in galactose, glucose and mannose, and the polar lipids mainly consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and phosphatidylinositol mannosides.
32924916	8	66	theme	Kitasatospora	1541:1553	arg1	isolate					1482:1488	the isolate	1478:1488	the isolate	1478:1488	The phylogenetic, chemotaxonomic and phenotypic properties enabled distinction of MMS16-CNU292T from related species, and thus the isolate should be recognized as a new species of the genus Kitasatospora, for which the name Kitasatospora acidiphila sp.
32924916	8	66	theme	Kitasatospora	1541:1553	arg1	species					1520:1526	a new species	1514:1526	a new species	1514:1526	The phylogenetic, chemotaxonomic and phenotypic properties enabled distinction of MMS16-CNU292T from related species, and thus the isolate should be recognized as a new species of the genus Kitasatospora, for which the name Kitasatospora acidiphila sp.
32924916	4	67	from	acid	796:799	arg1	peptidoglycan					818:830	the cell-wall peptidoglycan	804:830	the cell-wall peptidoglycan	804:830	Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan.
32924916	5	68	theme	polar	914:918	arg1	lipids					920:925	the polar lipids	910:925	the polar lipids mainly consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and phosphatidylinositol mannosides	910:1074	The whole-cell hydrolysates were rich in galactose, glucose and mannose, and the polar lipids mainly consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and phosphatidylinositol mannosides.
32924916	8	69	from	species	1460:1466	arg1	distinction					1418:1428	distinction	1418:1428	distinction of MMS16-CNU292T from related species	1418:1466	The phylogenetic, chemotaxonomic and phenotypic properties enabled distinction of MMS16-CNU292T from related species, and thus the isolate should be recognized as a new species of the genus Kitasatospora, for which the name Kitasatospora acidiphila sp.
32924916	2	70	dep	isolate	191:197	arg1	assigned					278:285	assigned	278:285	provisionally assigned to the genus Kitasatospora	264:312	A polyphasic study was carried out to establish the taxonomic position of an acidophilic isolate designated MMS16-CNU292T (=JCM 32302T) from pine grove soil, and provisionally assigned to the genus Kitasatospora.
32924916	2	70	dep	isolate	191:197	arg1	designated					199:208	designated	199:208	designated MMS16-CNU292T (=JCM 32302T) from pine grove soil	199:257	A polyphasic study was carried out to establish the taxonomic position of an acidophilic isolate designated MMS16-CNU292T (=JCM 32302T) from pine grove soil, and provisionally assigned to the genus Kitasatospora.
31800022	0	0	theme	Tandem	110:115	arg1	Spectrometry					122:133	Quadrupole Time-of-Flight Tandem Mass Spectrometry	84:133	Quadrupole Time-of-Flight Tandem Mass Spectrometry	84:133	Chemical Profiling of Embelia ribes by Ultra-High-Performance Liquid Chromatography Quadrupole Time-of-Flight Tandem Mass Spectrometry and Its Antioxidant and Anti-inflammatory Activities In Vitro.
31800022	5	1	theme	ideal	791:795	arg1	extract					764:770	the acetic ether extract	747:770	the acetic ether extract of E. ribes	747:782	Furthermore, the total phenolic and total flavonoid content was also assessed; the acetic ether extract of E. ribes was an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract).
31800022	5	1	theme	ideal	791:795	arg1	source					797:802	an ideal source	788:802	an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract)	788:935	Furthermore, the total phenolic and total flavonoid content was also assessed; the acetic ether extract of E. ribes was an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract).
31800022	7	2	from	cytokines	1310:1318	arg1	cells					1279:1283	lipopolysaccharide-simulated macrophage RAW 264.7 cells	1229:1283	lipopolysaccharide-simulated macrophage RAW 264.7 cells	1229:1283	Further, the nitric oxide concentration in lipopolysaccharide-simulated macrophage RAW 264.7 cells and the pro-inflammatory cytokines (TNF-α and IL-6) were suppressed by acetic ether extract.
31800022	7	3	theme	acetic	1356:1361	arg1	extract					1369:1375	acetic ether extract	1356:1375	acetic ether extract	1356:1375	Further, the nitric oxide concentration in lipopolysaccharide-simulated macrophage RAW 264.7 cells and the pro-inflammatory cytokines (TNF-α and IL-6) were suppressed by acetic ether extract.
31800022	5	4	theme	ribes	778:782	arg1	extract					764:770	the acetic ether extract	747:770	the acetic ether extract of E. ribes	747:782	Furthermore, the total phenolic and total flavonoid content was also assessed; the acetic ether extract of E. ribes was an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract).
31800022	5	4	theme	ribes	778:782	arg1	source					797:802	an ideal source	788:802	an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract)	788:935	Furthermore, the total phenolic and total flavonoid content was also assessed; the acetic ether extract of E. ribes was an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract).
31800022	1	5	theme	various	276:282	arg1	diseases					284:291	various diseases	276:291	various diseases	276:291	Embelia ribes is a traditional Chinese medicine compound used as a remedy for various diseases.
31800022	0	6	theme	Time-of-Flight	95:108	arg1	Spectrometry					122:133	Quadrupole Time-of-Flight Tandem Mass Spectrometry	84:133	Quadrupole Time-of-Flight Tandem Mass Spectrometry	84:133	Chemical Profiling of Embelia ribes by Ultra-High-Performance Liquid Chromatography Quadrupole Time-of-Flight Tandem Mass Spectrometry and Its Antioxidant and Anti-inflammatory Activities In Vitro.
31800022	7	7	theme	lipopolysaccharide-simulated	1229:1256	arg1	cells					1279:1283	lipopolysaccharide-simulated macrophage RAW 264.7 cells	1229:1283	lipopolysaccharide-simulated macrophage RAW 264.7 cells	1229:1283	Further, the nitric oxide concentration in lipopolysaccharide-simulated macrophage RAW 264.7 cells and the pro-inflammatory cytokines (TNF-α and IL-6) were suppressed by acetic ether extract.
31800022	1	8	theme	Embelia	198:204	arg1	ribes					206:210	Embelia ribes	198:210	Embelia ribes	198:210	Embelia ribes is a traditional Chinese medicine compound used as a remedy for various diseases.
31800022	3	9	theme	chromatography	418:431	arg1	spectrometry					471:482	ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry	388:482	ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry	388:482	Herein, ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry was used to characterize the components of E. ribes.
31800022	6	10	dep	power	1037:1041	arg1	0.06 ± 0.01 mg/mL					1166:1182	0.06 ± 0.01 mg/mL	1166:1182	ferric reducing activity power: 0.15 ± 0.01 mg/mL; 1,1-diphenyl-2-picrylhydrazyl: 0.18 ± 0.01 mg/mL; 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid: 0.06 ± 0.01 mg/mL	1012:1182	Additionally, acetic ether extract exhibited a high antioxidation effect (ferric reducing activity power: 0.15 ± 0.01 mg/mL; 1,1-diphenyl-2-picrylhydrazyl: 0.18 ± 0.01 mg/mL; 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid: 0.06 ± 0.01 mg/mL).
31800022	6	10	dep	power	1037:1041	arg1	0.18 ± 0.01 mg/mL					1094:1110	0.18 ± 0.01 mg/mL	1094:1110	ferric reducing activity power: 0.15 ± 0.01 mg/mL; 1,1-diphenyl-2-picrylhydrazyl: 0.18 ± 0.01 mg/mL; 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid: 0.06 ± 0.01 mg/mL	1012:1182	Additionally, acetic ether extract exhibited a high antioxidation effect (ferric reducing activity power: 0.15 ± 0.01 mg/mL; 1,1-diphenyl-2-picrylhydrazyl: 0.18 ± 0.01 mg/mL; 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid: 0.06 ± 0.01 mg/mL).
31800022	6	10	dep	power	1037:1041	arg1	acid					1160:1163	2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid	1113:1163	ferric reducing activity power: 0.15 ± 0.01 mg/mL; 1,1-diphenyl-2-picrylhydrazyl: 0.18 ± 0.01 mg/mL; 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid: 0.06 ± 0.01 mg/mL	1012:1182	Additionally, acetic ether extract exhibited a high antioxidation effect (ferric reducing activity power: 0.15 ± 0.01 mg/mL; 1,1-diphenyl-2-picrylhydrazyl: 0.18 ± 0.01 mg/mL; 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid: 0.06 ± 0.01 mg/mL).
31800022	6	10	dep	power	1037:1041	arg1	1,1-diphenyl-2-picrylhydrazyl					1063:1091	1,1-diphenyl-2-picrylhydrazyl	1063:1091	ferric reducing activity power: 0.15 ± 0.01 mg/mL; 1,1-diphenyl-2-picrylhydrazyl: 0.18 ± 0.01 mg/mL; 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid: 0.06 ± 0.01 mg/mL	1012:1182	Additionally, acetic ether extract exhibited a high antioxidation effect (ferric reducing activity power: 0.15 ± 0.01 mg/mL; 1,1-diphenyl-2-picrylhydrazyl: 0.18 ± 0.01 mg/mL; 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid: 0.06 ± 0.01 mg/mL).
31800022	6	10	dep	power	1037:1041	arg1	0.15 ± 0.01 mg/mL					1044:1060	0.15 ± 0.01 mg/mL	1044:1060	ferric reducing activity power: 0.15 ± 0.01 mg/mL; 1,1-diphenyl-2-picrylhydrazyl: 0.18 ± 0.01 mg/mL; 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid: 0.06 ± 0.01 mg/mL	1012:1182	Additionally, acetic ether extract exhibited a high antioxidation effect (ferric reducing activity power: 0.15 ± 0.01 mg/mL; 1,1-diphenyl-2-picrylhydrazyl: 0.18 ± 0.01 mg/mL; 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid: 0.06 ± 0.01 mg/mL).
31800022	5	11	theme	total	704:708	arg1	content					720:726	the total phenolic and total flavonoid content	681:726	content	720:726	Furthermore, the total phenolic and total flavonoid content was also assessed; the acetic ether extract of E. ribes was an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract).
31800022	8	12	theme	anti-inflammatory	1454:1470	arg1	agent					1472:1476	anti-inflammatory agent	1454:1476	anti-inflammatory agent	1454:1476	These findings support the notion that E. ribes is an ideal antioxidant and anti-inflammatory agent.
31800022	4	13	theme	other	635:639	arg1	compounds					641:649	15 other compounds	632:649	15 other compounds	632:649	A total of 56 compounds, including 16 phenolics, 16 flavonoids, 4 coumarins, 5 fatty acids and 15 other compounds were identified.
31800022	0	14	theme	Mass	117:120	arg1	Spectrometry					122:133	Quadrupole Time-of-Flight Tandem Mass Spectrometry	84:133	Quadrupole Time-of-Flight Tandem Mass Spectrometry	84:133	Chemical Profiling of Embelia ribes by Ultra-High-Performance Liquid Chromatography Quadrupole Time-of-Flight Tandem Mass Spectrometry and Its Antioxidant and Anti-inflammatory Activities In Vitro.
31800022	5	15	theme	flavonoid	710:718	arg1	content					720:726	the total phenolic and total flavonoid content	681:726	content	720:726	Furthermore, the total phenolic and total flavonoid content was also assessed; the acetic ether extract of E. ribes was an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract).
31800022	7	16	theme	ether	1363:1367	arg1	extract					1369:1375	acetic ether extract	1356:1375	acetic ether extract	1356:1375	Further, the nitric oxide concentration in lipopolysaccharide-simulated macrophage RAW 264.7 cells and the pro-inflammatory cytokines (TNF-α and IL-6) were suppressed by acetic ether extract.
31800022	6	17	theme	reducing	1019:1026	arg1	power					1037:1041	ferric reducing activity power	1012:1041	ferric reducing activity power: 0.15 ± 0.01 mg/mL; 1,1-diphenyl-2-picrylhydrazyl: 0.18 ± 0.01 mg/mL; 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid: 0.06 ± 0.01 mg/mL	1012:1182	Additionally, acetic ether extract exhibited a high antioxidation effect (ferric reducing activity power: 0.15 ± 0.01 mg/mL; 1,1-diphenyl-2-picrylhydrazyl: 0.18 ± 0.01 mg/mL; 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid: 0.06 ± 0.01 mg/mL).
31800022	3	18	theme	ultra-high-performance	388:409	arg1	spectrometry					471:482	ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry	388:482	ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry	388:482	Herein, ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry was used to characterize the components of E. ribes.
31800022	6	19	theme	acetic	952:957	arg1	extract					965:971	acetic ether extract	952:971	acetic ether extract	952:971	Additionally, acetic ether extract exhibited a high antioxidation effect (ferric reducing activity power: 0.15 ± 0.01 mg/mL; 1,1-diphenyl-2-picrylhydrazyl: 0.18 ± 0.01 mg/mL; 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid: 0.06 ± 0.01 mg/mL).
31800022	5	20	theme	gallic	835:840	arg1	equivalents/g					847:859	308.16 ± 0.00 mg gallic acid equivalents/g	818:859	308.16 ± 0.00 mg gallic acid equivalents/g of extract	818:870	Furthermore, the total phenolic and total flavonoid content was also assessed; the acetic ether extract of E. ribes was an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract).
31800022	2	21	theme	detailed	308:315	arg1	information					317:327	detailed information	308:327	detailed information regarding its chemical composition	308:362	Nevertheless, detailed information regarding its chemical composition is unavailable.
31800022	6	22	theme	2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic	1113:1158	arg1	acid					1160:1163	2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid	1113:1163	ferric reducing activity power: 0.15 ± 0.01 mg/mL; 1,1-diphenyl-2-picrylhydrazyl: 0.18 ± 0.01 mg/mL; 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid: 0.06 ± 0.01 mg/mL	1012:1182	Additionally, acetic ether extract exhibited a high antioxidation effect (ferric reducing activity power: 0.15 ± 0.01 mg/mL; 1,1-diphenyl-2-picrylhydrazyl: 0.18 ± 0.01 mg/mL; 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid: 0.06 ± 0.01 mg/mL).
31800022	3	23	theme	liquid	411:416	arg1	spectrometry					471:482	ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry	388:482	ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry	388:482	Herein, ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry was used to characterize the components of E. ribes.
31800022	7	24	theme	nitric	1199:1204	arg1	oxide					1206:1210	nitric oxide	1199:1210	the nitric oxide concentration in lipopolysaccharide-simulated macrophage RAW 264.7 cells	1195:1283	Further, the nitric oxide concentration in lipopolysaccharide-simulated macrophage RAW 264.7 cells and the pro-inflammatory cytokines (TNF-α and IL-6) were suppressed by acetic ether extract.
31800022	7	25	theme	macrophage	1258:1267	arg1	cells					1279:1283	lipopolysaccharide-simulated macrophage RAW 264.7 cells	1229:1283	lipopolysaccharide-simulated macrophage RAW 264.7 cells	1229:1283	Further, the nitric oxide concentration in lipopolysaccharide-simulated macrophage RAW 264.7 cells and the pro-inflammatory cytokines (TNF-α and IL-6) were suppressed by acetic ether extract.
31800022	5	26	theme	acid	842:845	arg1	equivalents/g					847:859	308.16 ± 0.00 mg gallic acid equivalents/g	818:859	308.16 ± 0.00 mg gallic acid equivalents/g of extract	818:870	Furthermore, the total phenolic and total flavonoid content was also assessed; the acetic ether extract of E. ribes was an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract).
31800022	3	27	theme	E.	527:528	arg1	ribes					530:534	E. ribes	527:534	E. ribes	527:534	Herein, ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry was used to characterize the components of E. ribes.
31800022	3	28	theme	mass	466:469	arg1	spectrometry					471:482	ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry	388:482	ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry	388:482	Herein, ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry was used to characterize the components of E. ribes.
31800022	4	29	theme	compounds	551:559	arg1	total					539:543	A total	537:543	A total of 56 compounds, including 16 phenolics, 16 flavonoids, 4 coumarins, 5 fatty acids and 15 other compounds	537:649	A total of 56 compounds, including 16 phenolics, 16 flavonoids, 4 coumarins, 5 fatty acids and 15 other compounds were identified.
31800022	7	30	theme	pro-inflammatory	1293:1308	arg1	IL-6					1331:1334	IL-6	1331:1334	IL-6	1331:1334	Further, the nitric oxide concentration in lipopolysaccharide-simulated macrophage RAW 264.7 cells and the pro-inflammatory cytokines (TNF-α and IL-6) were suppressed by acetic ether extract.
31800022	7	30	theme	pro-inflammatory	1293:1308	arg1	TNF-α					1321:1325	TNF-α	1321:1325	TNF-α	1321:1325	Further, the nitric oxide concentration in lipopolysaccharide-simulated macrophage RAW 264.7 cells and the pro-inflammatory cytokines (TNF-α and IL-6) were suppressed by acetic ether extract.
31800022	7	30	theme	pro-inflammatory	1293:1308	arg1	cytokines					1310:1318	the pro-inflammatory cytokines	1289:1318	the pro-inflammatory cytokines (TNF-α and IL-6)	1289:1335	Further, the nitric oxide concentration in lipopolysaccharide-simulated macrophage RAW 264.7 cells and the pro-inflammatory cytokines (TNF-α and IL-6) were suppressed by acetic ether extract.
31800022	5	31	theme	rutin	905:909	arg1	equivalents/g					911:923	62.00 ± 0.01 mg rutin equivalents/g	889:923	62.00 ± 0.01 mg rutin equivalents/g of extract	889:934	Furthermore, the total phenolic and total flavonoid content was also assessed; the acetic ether extract of E. ribes was an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract).
31800022	0	32	theme	Chemical	0:7	arg1	Profiling					9:17	Chemical Profiling	0:17	Chemical Profiling of Embelia	0:28	Chemical Profiling of Embelia ribes by Ultra-High-Performance Liquid Chromatography Quadrupole Time-of-Flight Tandem Mass Spectrometry and Its Antioxidant and Anti-inflammatory Activities In Vitro.
31800022	6	33	theme	ferric	1012:1017	arg1	power					1037:1041	ferric reducing activity power	1012:1041	ferric reducing activity power: 0.15 ± 0.01 mg/mL; 1,1-diphenyl-2-picrylhydrazyl: 0.18 ± 0.01 mg/mL; 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid: 0.06 ± 0.01 mg/mL	1012:1182	Additionally, acetic ether extract exhibited a high antioxidation effect (ferric reducing activity power: 0.15 ± 0.01 mg/mL; 1,1-diphenyl-2-picrylhydrazyl: 0.18 ± 0.01 mg/mL; 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid: 0.06 ± 0.01 mg/mL).
31800022	5	34	dep	phenolics	807:815	arg1	equivalents/g					847:859	308.16 ± 0.00 mg gallic acid equivalents/g	818:859	308.16 ± 0.00 mg gallic acid equivalents/g of extract	818:870	Furthermore, the total phenolic and total flavonoid content was also assessed; the acetic ether extract of E. ribes was an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract).
31800022	1	35	theme	traditional	217:227	arg1	medicine					237:244	a traditional Chinese medicine	215:244	a traditional Chinese medicine compound used as a remedy for various diseases	215:291	Embelia ribes is a traditional Chinese medicine compound used as a remedy for various diseases.
31800022	0	36	theme	Embelia	22:28	arg1	Profiling					9:17	Chemical Profiling	0:17	Chemical Profiling of Embelia	0:28	Chemical Profiling of Embelia ribes by Ultra-High-Performance Liquid Chromatography Quadrupole Time-of-Flight Tandem Mass Spectrometry and Its Antioxidant and Anti-inflammatory Activities In Vitro.
31800022	2	37	theme	chemical	343:350	arg1	composition					352:362	its chemical composition	339:362	its chemical composition	339:362	Nevertheless, detailed information regarding its chemical composition is unavailable.
31800022	1	38	theme	Chinese	229:235	arg1	medicine					237:244	a traditional Chinese medicine	215:244	a traditional Chinese medicine compound used as a remedy for various diseases	215:291	Embelia ribes is a traditional Chinese medicine compound used as a remedy for various diseases.
31800022	3	39	theme	tandem	459:464	arg1	spectrometry					471:482	ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry	388:482	ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry	388:482	Herein, ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry was used to characterize the components of E. ribes.
31800022	4	40	theme	fatty	616:620	arg1	acids					622:626	5 fatty acids	614:626	5 fatty acids	614:626	A total of 56 compounds, including 16 phenolics, 16 flavonoids, 4 coumarins, 5 fatty acids and 15 other compounds were identified.
31800022	0	41	theme	Antioxidant	143:153	arg1	Activities					177:186	Its Antioxidant and Anti-inflammatory Activities	139:186	Its Antioxidant and Anti-inflammatory Activities	139:186	Chemical Profiling of Embelia ribes by Ultra-High-Performance Liquid Chromatography Quadrupole Time-of-Flight Tandem Mass Spectrometry and Its Antioxidant and Anti-inflammatory Activities In Vitro.
31800022	0	42	dep	Chromatography	69:82	arg1	Spectrometry					122:133	Quadrupole Time-of-Flight Tandem Mass Spectrometry	84:133	Quadrupole Time-of-Flight Tandem Mass Spectrometry	84:133	Chemical Profiling of Embelia ribes by Ultra-High-Performance Liquid Chromatography Quadrupole Time-of-Flight Tandem Mass Spectrometry and Its Antioxidant and Anti-inflammatory Activities In Vitro.
31800022	5	43	theme	308.16 ± 0.00 mg	818:833	arg1	equivalents/g					847:859	308.16 ± 0.00 mg gallic acid equivalents/g	818:859	308.16 ± 0.00 mg gallic acid equivalents/g of extract	818:870	Furthermore, the total phenolic and total flavonoid content was also assessed; the acetic ether extract of E. ribes was an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract).
31800022	1	44	theme	medicine	237:244	arg1	compound					246:253	a traditional Chinese medicine compound	215:253	a traditional Chinese medicine compound used as a remedy for various diseases	215:291	Embelia ribes is a traditional Chinese medicine compound used as a remedy for various diseases.
31800022	6	45	theme	antioxidation	990:1002	arg1	effect					1004:1009	a high antioxidation effect	983:1009	a high antioxidation effect (ferric reducing activity power: 0.15 ± 0.01 mg/mL; 1,1-diphenyl-2-picrylhydrazyl: 0.18 ± 0.01 mg/mL; 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid: 0.06 ± 0.01 mg/mL)	983:1183	Additionally, acetic ether extract exhibited a high antioxidation effect (ferric reducing activity power: 0.15 ± 0.01 mg/mL; 1,1-diphenyl-2-picrylhydrazyl: 0.18 ± 0.01 mg/mL; 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid: 0.06 ± 0.01 mg/mL).
31800022	5	46	theme	acetic	751:756	arg1	extract					764:770	the acetic ether extract	747:770	the acetic ether extract of E. ribes	747:782	Furthermore, the total phenolic and total flavonoid content was also assessed; the acetic ether extract of E. ribes was an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract).
31800022	5	46	theme	acetic	751:756	arg1	source					797:802	an ideal source	788:802	an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract)	788:935	Furthermore, the total phenolic and total flavonoid content was also assessed; the acetic ether extract of E. ribes was an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract).
31800022	5	47	theme	ether	758:762	arg1	extract					764:770	the acetic ether extract	747:770	the acetic ether extract of E. ribes	747:782	Furthermore, the total phenolic and total flavonoid content was also assessed; the acetic ether extract of E. ribes was an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract).
31800022	5	47	theme	ether	758:762	arg1	source					797:802	an ideal source	788:802	an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract)	788:935	Furthermore, the total phenolic and total flavonoid content was also assessed; the acetic ether extract of E. ribes was an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract).
31800022	3	48	theme	ribes	530:534	arg1	components					513:522	the components	509:522	the components of E. ribes	509:534	Herein, ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry was used to characterize the components of E. ribes.
31800022	5	49	theme	phenolics	807:815	arg1	extract					764:770	the acetic ether extract	747:770	the acetic ether extract of E. ribes	747:782	Furthermore, the total phenolic and total flavonoid content was also assessed; the acetic ether extract of E. ribes was an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract).
31800022	5	49	theme	phenolics	807:815	arg1	source					797:802	an ideal source	788:802	an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract)	788:935	Furthermore, the total phenolic and total flavonoid content was also assessed; the acetic ether extract of E. ribes was an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract).
31800022	6	50	theme	high	985:988	arg1	effect					1004:1009	a high antioxidation effect	983:1009	a high antioxidation effect (ferric reducing activity power: 0.15 ± 0.01 mg/mL; 1,1-diphenyl-2-picrylhydrazyl: 0.18 ± 0.01 mg/mL; 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid: 0.06 ± 0.01 mg/mL)	983:1183	Additionally, acetic ether extract exhibited a high antioxidation effect (ferric reducing activity power: 0.15 ± 0.01 mg/mL; 1,1-diphenyl-2-picrylhydrazyl: 0.18 ± 0.01 mg/mL; 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid: 0.06 ± 0.01 mg/mL).
31800022	0	51	theme	Anti-inflammatory	159:175	arg1	Activities					177:186	Its Antioxidant and Anti-inflammatory Activities	139:186	Its Antioxidant and Anti-inflammatory Activities	139:186	Chemical Profiling of Embelia ribes by Ultra-High-Performance Liquid Chromatography Quadrupole Time-of-Flight Tandem Mass Spectrometry and Its Antioxidant and Anti-inflammatory Activities In Vitro.
31800022	3	52	theme	time-of-flight	444:457	arg1	spectrometry					471:482	ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry	388:482	ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry	388:482	Herein, ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry was used to characterize the components of E. ribes.
31800022	8	53	theme	ideal	1432:1436	arg1	ribes					1420:1424	E. ribes	1417:1424	E. ribes	1417:1424	These findings support the notion that E. ribes is an ideal antioxidant and anti-inflammatory agent.
31800022	8	53	theme	ideal	1432:1436	arg1	antioxidant					1438:1448	an ideal antioxidant	1429:1448	an ideal antioxidant	1429:1448	These findings support the notion that E. ribes is an ideal antioxidant and anti-inflammatory agent.
31800022	5	54	theme	flavonoids	877:886	arg1	extract					764:770	the acetic ether extract	747:770	the acetic ether extract of E. ribes	747:782	Furthermore, the total phenolic and total flavonoid content was also assessed; the acetic ether extract of E. ribes was an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract).
31800022	5	54	theme	flavonoids	877:886	arg1	source					797:802	an ideal source	788:802	an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract)	788:935	Furthermore, the total phenolic and total flavonoid content was also assessed; the acetic ether extract of E. ribes was an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract).
31800022	0	55	theme	Liquid	62:67	arg1	Chromatography					69:82	Ultra-High-Performance Liquid Chromatography Quadrupole Time-of-Flight Tandem Mass Spectrometry and Its Antioxidant and Anti-inflammatory Activities	39:186	Chromatography	69:82	Chemical Profiling of Embelia ribes by Ultra-High-Performance Liquid Chromatography Quadrupole Time-of-Flight Tandem Mass Spectrometry and Its Antioxidant and Anti-inflammatory Activities In Vitro.
31800022	7	56	from	concentration	1212:1224	arg1	cells					1279:1283	lipopolysaccharide-simulated macrophage RAW 264.7 cells	1229:1283	lipopolysaccharide-simulated macrophage RAW 264.7 cells	1229:1283	Further, the nitric oxide concentration in lipopolysaccharide-simulated macrophage RAW 264.7 cells and the pro-inflammatory cytokines (TNF-α and IL-6) were suppressed by acetic ether extract.
31800022	3	57	used	used	488:491	arg2	spectrometry					471:482	ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry	388:482	ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry	388:482	Herein, ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry was used to characterize the components of E. ribes.
31800022	0	58	theme	Ultra-High-Performance	39:60	arg1	Chromatography					69:82	Ultra-High-Performance Liquid Chromatography Quadrupole Time-of-Flight Tandem Mass Spectrometry and Its Antioxidant and Anti-inflammatory Activities	39:186	Chromatography	69:82	Chemical Profiling of Embelia ribes by Ultra-High-Performance Liquid Chromatography Quadrupole Time-of-Flight Tandem Mass Spectrometry and Its Antioxidant and Anti-inflammatory Activities In Vitro.
31800022	6	59	dep	effect	1004:1009	arg1	power					1037:1041	ferric reducing activity power	1012:1041	ferric reducing activity power: 0.15 ± 0.01 mg/mL; 1,1-diphenyl-2-picrylhydrazyl: 0.18 ± 0.01 mg/mL; 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid: 0.06 ± 0.01 mg/mL	1012:1182	Additionally, acetic ether extract exhibited a high antioxidation effect (ferric reducing activity power: 0.15 ± 0.01 mg/mL; 1,1-diphenyl-2-picrylhydrazyl: 0.18 ± 0.01 mg/mL; 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid: 0.06 ± 0.01 mg/mL).
31800022	7	60	theme	oxide	1206:1210	arg1	concentration					1212:1224	the nitric oxide concentration	1195:1224	the nitric oxide concentration in lipopolysaccharide-simulated macrophage RAW 264.7 cells	1195:1283	Further, the nitric oxide concentration in lipopolysaccharide-simulated macrophage RAW 264.7 cells and the pro-inflammatory cytokines (TNF-α and IL-6) were suppressed by acetic ether extract.
31800022	7	61	dep	cytokines	1310:1318	arg1	IL-6					1331:1334	IL-6	1331:1334	IL-6	1331:1334	Further, the nitric oxide concentration in lipopolysaccharide-simulated macrophage RAW 264.7 cells and the pro-inflammatory cytokines (TNF-α and IL-6) were suppressed by acetic ether extract.
31800022	7	61	dep	cytokines	1310:1318	arg1	TNF-α					1321:1325	TNF-α	1321:1325	TNF-α	1321:1325	Further, the nitric oxide concentration in lipopolysaccharide-simulated macrophage RAW 264.7 cells and the pro-inflammatory cytokines (TNF-α and IL-6) were suppressed by acetic ether extract.
31800022	7	61	dep	cytokines	1310:1318	arg1	cytokines					1310:1318	the pro-inflammatory cytokines	1289:1318	the pro-inflammatory cytokines (TNF-α and IL-6)	1289:1335	Further, the nitric oxide concentration in lipopolysaccharide-simulated macrophage RAW 264.7 cells and the pro-inflammatory cytokines (TNF-α and IL-6) were suppressed by acetic ether extract.
31800022	7	62	theme	RAW	1269:1271	arg1	cells					1279:1283	lipopolysaccharide-simulated macrophage RAW 264.7 cells	1229:1283	lipopolysaccharide-simulated macrophage RAW 264.7 cells	1229:1283	Further, the nitric oxide concentration in lipopolysaccharide-simulated macrophage RAW 264.7 cells and the pro-inflammatory cytokines (TNF-α and IL-6) were suppressed by acetic ether extract.
31800022	5	63	dep	flavonoids	877:886	arg1	equivalents/g					911:923	62.00 ± 0.01 mg rutin equivalents/g	889:923	62.00 ± 0.01 mg rutin equivalents/g of extract	889:934	Furthermore, the total phenolic and total flavonoid content was also assessed; the acetic ether extract of E. ribes was an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract).
31800022	0	64	theme	Quadrupole	84:93	arg1	Spectrometry					122:133	Quadrupole Time-of-Flight Tandem Mass Spectrometry	84:133	Quadrupole Time-of-Flight Tandem Mass Spectrometry	84:133	Chemical Profiling of Embelia ribes by Ultra-High-Performance Liquid Chromatography Quadrupole Time-of-Flight Tandem Mass Spectrometry and Its Antioxidant and Anti-inflammatory Activities In Vitro.
31800022	5	65	theme	extract	864:870	arg1	equivalents/g					847:859	308.16 ± 0.00 mg gallic acid equivalents/g	818:859	308.16 ± 0.00 mg gallic acid equivalents/g of extract	818:870	Furthermore, the total phenolic and total flavonoid content was also assessed; the acetic ether extract of E. ribes was an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract).
31800022	5	66	theme	62.00 ± 0.01 mg	889:903	arg1	equivalents/g					911:923	62.00 ± 0.01 mg rutin equivalents/g	889:923	62.00 ± 0.01 mg rutin equivalents/g of extract	889:934	Furthermore, the total phenolic and total flavonoid content was also assessed; the acetic ether extract of E. ribes was an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract).
31800022	6	67	theme	activity	1028:1035	arg1	power					1037:1041	ferric reducing activity power	1012:1041	ferric reducing activity power: 0.15 ± 0.01 mg/mL; 1,1-diphenyl-2-picrylhydrazyl: 0.18 ± 0.01 mg/mL; 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid: 0.06 ± 0.01 mg/mL	1012:1182	Additionally, acetic ether extract exhibited a high antioxidation effect (ferric reducing activity power: 0.15 ± 0.01 mg/mL; 1,1-diphenyl-2-picrylhydrazyl: 0.18 ± 0.01 mg/mL; 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid: 0.06 ± 0.01 mg/mL).
31800022	6	68	theme	ether	959:963	arg1	extract					965:971	acetic ether extract	952:971	acetic ether extract	952:971	Additionally, acetic ether extract exhibited a high antioxidation effect (ferric reducing activity power: 0.15 ± 0.01 mg/mL; 1,1-diphenyl-2-picrylhydrazyl: 0.18 ± 0.01 mg/mL; 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid: 0.06 ± 0.01 mg/mL).
31800022	5	69	theme	extract	928:934	arg1	equivalents/g					911:923	62.00 ± 0.01 mg rutin equivalents/g	889:923	62.00 ± 0.01 mg rutin equivalents/g of extract	889:934	Furthermore, the total phenolic and total flavonoid content was also assessed; the acetic ether extract of E. ribes was an ideal source of phenolics (308.16 ± 0.00 mg gallic acid equivalents/g of extract) and flavonoids (62.00 ± 0.01 mg rutin equivalents/g of extract).
31800022	3	70	theme	quadrupole	433:442	arg1	spectrometry					471:482	ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry	388:482	ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry	388:482	Herein, ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry was used to characterize the components of E. ribes.
34684567	0	0	theme	Inflammatory	93:104	arg1	Disease					112:118	Inflammatory Bowel Disease	93:118	Inflammatory Bowel Disease Microbiota	93:129	The Emulsifier Carboxymethylcellulose Induces More Aggressive Colitis in Humanized Mice with Inflammatory Bowel Disease Microbiota Than Polysorbate-80.
34684567	6	1	dep	P80	999:1001	arg1	treatment					1013:1021	treatment	1013:1021	treatment	1013:1021	CMC treatment significantly increased fecal Lcn-2 levels compared to P80 and water treatment by one week and throughout the experiment.
34684567	7	2	theme	inflammatory	1143:1154	arg1	expression					1170:1179	colonic inflammatory cytokine gene expression	1135:1179	colonic inflammatory cytokine gene expression	1135:1179	Likewise, CMC treatment increased histologic inflammatory scores and colonic inflammatory cytokine gene expression compared with P80 and water controls.
34684567	1	3	theme	intestinal	270:279	arg1	inflammation					281:292	intestinal inflammation	270:292	intestinal inflammation	270:292	Commonly used synthetic dietary emulsifiers, including carboxymethylcellulose (CMC) and polysorbate-80 (P80), promote intestinal inflammation.
34684567	2	4	theme	fecal	495:499	arg1	transplant					501:510	pooled fecal transplant	488:510	pooled fecal transplant from three patients with active inflammatory bowel diseases	488:570	We compared abilities of CMC vs. P80 to potentiate colitis and impact human microbiota in an inflammatory environment using a novel colitis model of ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases.
34684567	7	5	theme	cytokine	1156:1163	arg1	expression					1170:1179	colonic inflammatory cytokine gene expression	1135:1179	colonic inflammatory cytokine gene expression	1135:1179	Likewise, CMC treatment increased histologic inflammatory scores and colonic inflammatory cytokine gene expression compared with P80 and water controls.
34684567	11	6	theme	bacterial	1656:1664	arg1	composition					1666:1676	bacterial composition	1656:1676	bacterial composition	1656:1676	CMC promotes more aggressive inflammation without changing bacterial composition.
34684567	5	7	theme	shotgun	898:904	arg1	sequencing					918:927	shotgun metagenomic sequencing	898:927	shotgun metagenomic sequencing	898:927	Microbiota profiles in cecal contents were determined by shotgun metagenomic sequencing.
34684567	7	8	dep	P80	1195:1197	arg1	controls					1209:1216	controls	1209:1216	controls	1209:1216	Likewise, CMC treatment increased histologic inflammatory scores and colonic inflammatory cytokine gene expression compared with P80 and water controls.
34684567	0	9	theme	Disease	112:118	arg1	Microbiota					120:129	Inflammatory Bowel Disease Microbiota	93:129	Inflammatory Bowel Disease Microbiota	93:129	The Emulsifier Carboxymethylcellulose Induces More Aggressive Colitis in Humanized Mice with Inflammatory Bowel Disease Microbiota Than Polysorbate-80.
34684567	2	10	with	patients	523:530	arg1	diseases					563:570	active inflammatory bowel diseases	537:570	active inflammatory bowel diseases	537:570	We compared abilities of CMC vs. P80 to potentiate colitis and impact human microbiota in an inflammatory environment using a novel colitis model of ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases.
34684567	2	11	from	colitis	346:352	arg1	environment					401:411	an inflammatory environment	385:411	an inflammatory environment using a novel colitis model of ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases	385:570	We compared abilities of CMC vs. P80 to potentiate colitis and impact human microbiota in an inflammatory environment using a novel colitis model of ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases.
34684567	2	12	theme	CMC	320:322	arg1	abilities					307:315	abilities	307:315	abilities of CMC vs. P80 to potentiate colitis and impact human microbiota in an inflammatory environment using a novel colitis model of ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases	307:570	We compared abilities of CMC vs. P80 to potentiate colitis and impact human microbiota in an inflammatory environment using a novel colitis model of ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases.
34684567	0	13	theme	Bowel	106:110	arg1	Disease					112:118	Inflammatory Bowel Disease	93:118	Inflammatory Bowel Disease Microbiota	93:129	The Emulsifier Carboxymethylcellulose Induces More Aggressive Colitis in Humanized Mice with Inflammatory Bowel Disease Microbiota Than Polysorbate-80.
34684567	7	14	theme	colonic	1135:1141	arg1	expression					1170:1179	colonic inflammatory cytokine gene expression	1135:1179	colonic inflammatory cytokine gene expression	1135:1179	Likewise, CMC treatment increased histologic inflammatory scores and colonic inflammatory cytokine gene expression compared with P80 and water controls.
34684567	0	15	with	Mice	83:86	arg1	Microbiota					120:129	Inflammatory Bowel Disease Microbiota	93:129	Inflammatory Bowel Disease Microbiota	93:129	The Emulsifier Carboxymethylcellulose Induces More Aggressive Colitis in Humanized Mice with Inflammatory Bowel Disease Microbiota Than Polysorbate-80.
34684567	0	16	from	Colitis	62:68	arg1	Mice					83:86	Humanized Mice	73:86	Humanized Mice with Inflammatory Bowel Disease Microbiota	73:129	The Emulsifier Carboxymethylcellulose Induces More Aggressive Colitis in Humanized Mice with Inflammatory Bowel Disease Microbiota Than Polysorbate-80.
34684567	7	17	theme	inflammatory	1111:1122	arg1	scores					1124:1129	histologic inflammatory scores	1100:1129	histologic inflammatory scores	1100:1129	Likewise, CMC treatment increased histologic inflammatory scores and colonic inflammatory cytokine gene expression compared with P80 and water controls.
34684567	10	18	theme	different	1540:1548	arg1	abilities					1550:1558	different abilities	1540:1558	different abilities to induce colitis in humanized mice	1540:1594	Commonly used dietary emulsifiers have different abilities to induce colitis in humanized mice.
34684567	2	19	theme	novel	421:425	arg1	model					435:439	a novel colitis model	419:439	a novel colitis model of ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases	419:570	We compared abilities of CMC vs. P80 to potentiate colitis and impact human microbiota in an inflammatory environment using a novel colitis model of ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases.
34684567	8	20	theme	specific	1263:1270	arg1	microbiota					1283:1292	specific intestinal microbiota	1263:1292	specific intestinal microbiota	1263:1292	The two emulsifiers differentially affected specific intestinal microbiota.
34684567	2	21	theme	pooled	488:493	arg1	transplant					501:510	pooled fecal transplant	488:510	pooled fecal transplant from three patients with active inflammatory bowel diseases	488:570	We compared abilities of CMC vs. P80 to potentiate colitis and impact human microbiota in an inflammatory environment using a novel colitis model of ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases.
34684567	10	22	theme	dietary	1515:1521	arg1	emulsifiers					1523:1533	Commonly used dietary emulsifiers	1501:1533	Commonly used dietary emulsifiers	1501:1533	Commonly used dietary emulsifiers have different abilities to induce colitis in humanized mice.
34684567	2	23	theme	IL10-/-	462:468	arg1	mice					470:473	ex-germ-free (GF) IL10-/- mice	444:473	ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases	444:570	We compared abilities of CMC vs. P80 to potentiate colitis and impact human microbiota in an inflammatory environment using a novel colitis model of ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases.
34684567	10	24	contain	have	1535:1538	arg1	emulsifiers					1523:1533	Commonly used dietary emulsifiers	1501:1533	Commonly used dietary emulsifiers	1501:1533	Commonly used dietary emulsifiers have different abilities to induce colitis in humanized mice.
34684567	10	24	contain	have	1535:1538	arg2	abilities					1550:1558	different abilities	1540:1558	different abilities to induce colitis in humanized mice	1540:1594	Commonly used dietary emulsifiers have different abilities to induce colitis in humanized mice.
34684567	0	25	theme	Emulsifier	4:13	arg1	Carboxymethylcellulose					15:36	The Emulsifier Carboxymethylcellulose	0:36	The Emulsifier Carboxymethylcellulose	0:36	The Emulsifier Carboxymethylcellulose Induces More Aggressive Colitis in Humanized Mice with Inflammatory Bowel Disease Microbiota Than Polysorbate-80.
34684567	6	26	theme	Lcn-2	974:978	arg1	levels					980:985	fecal Lcn-2 levels	968:985	fecal Lcn-2 levels	968:985	CMC treatment significantly increased fecal Lcn-2 levels compared to P80 and water treatment by one week and throughout the experiment.
34684567	2	27	from	microbiota	371:380	arg1	environment					401:411	an inflammatory environment	385:411	an inflammatory environment using a novel colitis model of ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases	385:570	We compared abilities of CMC vs. P80 to potentiate colitis and impact human microbiota in an inflammatory environment using a novel colitis model of ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases.
34684567	5	28	theme	cecal	864:868	arg1	contents					870:877	cecal contents	864:877	cecal contents	864:877	Microbiota profiles in cecal contents were determined by shotgun metagenomic sequencing.
34684567	7	29	theme	histologic	1100:1109	arg1	scores					1124:1129	histologic inflammatory scores	1100:1129	histologic inflammatory scores	1100:1129	Likewise, CMC treatment increased histologic inflammatory scores and colonic inflammatory cytokine gene expression compared with P80 and water controls.
34684567	9	30	theme	Proteobacteria	1485:1498	arg1	abundance					1448:1456	the abundance	1444:1456	the abundance of both Actinobacteria and Proteobacteria	1444:1498	CMC did not impact bacterial composition but significantly decreased Caudoviricetes (bacteriophages), while P80 exposure non-significantly increased the abundance of both Actinobacteria and Proteobacteria.
34684567	2	31	theme	GF	458:459	arg1	mice					470:473	ex-germ-free (GF) IL10-/- mice	444:473	ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases	444:570	We compared abilities of CMC vs. P80 to potentiate colitis and impact human microbiota in an inflammatory environment using a novel colitis model of ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases.
34684567	9	32	theme	P80	1403:1405	arg1	exposure					1407:1414	P80 exposure	1403:1414	P80 exposure	1403:1414	CMC did not impact bacterial composition but significantly decreased Caudoviricetes (bacteriophages), while P80 exposure non-significantly increased the abundance of both Actinobacteria and Proteobacteria.
34684567	7	33	theme	CMC	1076:1078	arg1	treatment					1080:1088	CMC treatment	1076:1088	CMC treatment	1076:1088	Likewise, CMC treatment increased histologic inflammatory scores and colonic inflammatory cytokine gene expression compared with P80 and water controls.
34684567	4	34	theme	inflammatory	802:813	arg1	expression					829:838	colonic inflammatory cytokine gene expression	794:838	colonic inflammatory cytokine gene expression	794:838	Inflammation was quantified by serial fecal lipocalin 2 (Lcn-2) and after four weeks by blinded colonic histologic scores and colonic inflammatory cytokine gene expression.
34684567	2	35	theme	P80	328:330	arg1	abilities					307:315	abilities	307:315	abilities of CMC vs. P80 to potentiate colitis and impact human microbiota in an inflammatory environment using a novel colitis model of ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases	307:570	We compared abilities of CMC vs. P80 to potentiate colitis and impact human microbiota in an inflammatory environment using a novel colitis model of ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases.
34684567	4	36	theme	colonic	794:800	arg1	expression					829:838	colonic inflammatory cytokine gene expression	794:838	colonic inflammatory cytokine gene expression	794:838	Inflammation was quantified by serial fecal lipocalin 2 (Lcn-2) and after four weeks by blinded colonic histologic scores and colonic inflammatory cytokine gene expression.
34684567	2	37	theme	ex-germ-free	444:455	arg1	mice					470:473	ex-germ-free (GF) IL10-/- mice	444:473	ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases	444:570	We compared abilities of CMC vs. P80 to potentiate colitis and impact human microbiota in an inflammatory environment using a novel colitis model of ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases.
34684567	9	38	theme	bacterial	1314:1322	arg1	composition					1324:1334	bacterial composition	1314:1334	bacterial composition	1314:1334	CMC did not impact bacterial composition but significantly decreased Caudoviricetes (bacteriophages), while P80 exposure non-significantly increased the abundance of both Actinobacteria and Proteobacteria.
34684567	1	39	theme	synthetic	166:174	arg1	polysorbate-80					240:253	polysorbate-80	240:253	polysorbate-80 (P80)	240:259	Commonly used synthetic dietary emulsifiers, including carboxymethylcellulose (CMC) and polysorbate-80 (P80), promote intestinal inflammation.
34684567	1	39	theme	synthetic	166:174	arg1	emulsifiers					184:194	Commonly used synthetic dietary emulsifiers	152:194	Commonly used synthetic dietary emulsifiers	152:194	Commonly used synthetic dietary emulsifiers, including carboxymethylcellulose (CMC) and polysorbate-80 (P80), promote intestinal inflammation.
34684567	1	39	theme	synthetic	166:174	arg1	carboxymethylcellulose					207:228	carboxymethylcellulose	207:228	carboxymethylcellulose (CMC)	207:234	Commonly used synthetic dietary emulsifiers, including carboxymethylcellulose (CMC) and polysorbate-80 (P80), promote intestinal inflammation.
34684567	0	40	theme	Aggressive	51:60	arg1	Colitis					62:68	More Aggressive Colitis	46:68	More Aggressive Colitis in Humanized Mice with Inflammatory Bowel Disease Microbiota Than Polysorbate-80	46:149	The Emulsifier Carboxymethylcellulose Induces More Aggressive Colitis in Humanized Mice with Inflammatory Bowel Disease Microbiota Than Polysorbate-80.
34684567	4	41	theme	serial	699:704	arg1	lipocalin					712:720	serial fecal lipocalin 2	699:722	serial fecal lipocalin 2 (Lcn-2)	699:730	Inflammation was quantified by serial fecal lipocalin 2 (Lcn-2) and after four weeks by blinded colonic histologic scores and colonic inflammatory cytokine gene expression.
34684567	4	41	theme	serial	699:704	arg1	Lcn-2					725:729	Lcn-2	725:729	Lcn-2	725:729	Inflammation was quantified by serial fecal lipocalin 2 (Lcn-2) and after four weeks by blinded colonic histologic scores and colonic inflammatory cytokine gene expression.
34684567	10	42	theme	used	1510:1513	arg1	emulsifiers					1523:1533	Commonly used dietary emulsifiers	1501:1533	Commonly used dietary emulsifiers	1501:1533	Commonly used dietary emulsifiers have different abilities to induce colitis in humanized mice.
34684567	3	43	theme	%	606:606	arg1	CMC					608:610	1% CMC	605:610	1% CMC	605:610	After three days, mice received 1% CMC or P80 in drinking water or water alone for four weeks.
34684567	6	44	theme	fecal	968:972	arg1	levels					980:985	fecal Lcn-2 levels	968:985	fecal Lcn-2 levels	968:985	CMC treatment significantly increased fecal Lcn-2 levels compared to P80 and water treatment by one week and throughout the experiment.
34684567	2	45	theme	bowel	557:561	arg1	diseases					563:570	active inflammatory bowel diseases	537:570	active inflammatory bowel diseases	537:570	We compared abilities of CMC vs. P80 to potentiate colitis and impact human microbiota in an inflammatory environment using a novel colitis model of ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases.
34684567	10	46	theme	humanized	1581:1589	arg1	mice					1591:1594	humanized mice	1581:1594	humanized mice	1581:1594	Commonly used dietary emulsifiers have different abilities to induce colitis in humanized mice.
34684567	2	47	theme	colitis	427:433	arg1	model					435:439	a novel colitis model	419:439	a novel colitis model of ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases	419:570	We compared abilities of CMC vs. P80 to potentiate colitis and impact human microbiota in an inflammatory environment using a novel colitis model of ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases.
34684567	7	48	theme	gene	1165:1168	arg1	expression					1170:1179	colonic inflammatory cytokine gene expression	1135:1179	colonic inflammatory cytokine gene expression	1135:1179	Likewise, CMC treatment increased histologic inflammatory scores and colonic inflammatory cytokine gene expression compared with P80 and water controls.
34684567	2	49	theme	inflammatory	544:555	arg1	diseases					563:570	active inflammatory bowel diseases	537:570	active inflammatory bowel diseases	537:570	We compared abilities of CMC vs. P80 to potentiate colitis and impact human microbiota in an inflammatory environment using a novel colitis model of ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases.
34684567	4	50	theme	cytokine	815:822	arg1	expression					829:838	colonic inflammatory cytokine gene expression	794:838	colonic inflammatory cytokine gene expression	794:838	Inflammation was quantified by serial fecal lipocalin 2 (Lcn-2) and after four weeks by blinded colonic histologic scores and colonic inflammatory cytokine gene expression.
34684567	2	51	theme	human	365:369	arg1	microbiota					371:380	impact human microbiota	358:380	impact human microbiota	358:380	We compared abilities of CMC vs. P80 to potentiate colitis and impact human microbiota in an inflammatory environment using a novel colitis model of ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases.
34684567	2	52	theme	mice	470:473	arg1	model					435:439	a novel colitis model	419:439	a novel colitis model of ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases	419:570	We compared abilities of CMC vs. P80 to potentiate colitis and impact human microbiota in an inflammatory environment using a novel colitis model of ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases.
34684567	4	53	theme	gene	824:827	arg1	expression					829:838	colonic inflammatory cytokine gene expression	794:838	colonic inflammatory cytokine gene expression	794:838	Inflammation was quantified by serial fecal lipocalin 2 (Lcn-2) and after four weeks by blinded colonic histologic scores and colonic inflammatory cytokine gene expression.
34684567	3	54	theme	1	605:605	arg1	%					606:606	%	606:606	%	606:606	After three days, mice received 1% CMC or P80 in drinking water or water alone for four weeks.
34684567	2	55	theme	impact	358:363	arg1	microbiota					371:380	impact human microbiota	358:380	impact human microbiota	358:380	We compared abilities of CMC vs. P80 to potentiate colitis and impact human microbiota in an inflammatory environment using a novel colitis model of ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases.
34684567	4	56	theme	fecal	706:710	arg1	lipocalin					712:720	serial fecal lipocalin 2	699:722	serial fecal lipocalin 2 (Lcn-2)	699:730	Inflammation was quantified by serial fecal lipocalin 2 (Lcn-2) and after four weeks by blinded colonic histologic scores and colonic inflammatory cytokine gene expression.
34684567	4	56	theme	fecal	706:710	arg1	Lcn-2					725:729	Lcn-2	725:729	Lcn-2	725:729	Inflammation was quantified by serial fecal lipocalin 2 (Lcn-2) and after four weeks by blinded colonic histologic scores and colonic inflammatory cytokine gene expression.
34684567	2	57	from	patients	523:530	arg1	transplant					501:510	pooled fecal transplant	488:510	pooled fecal transplant from three patients with active inflammatory bowel diseases	488:570	We compared abilities of CMC vs. P80 to potentiate colitis and impact human microbiota in an inflammatory environment using a novel colitis model of ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases.
34684567	4	58	theme	colonic	764:770	arg1	scores					783:788	blinded colonic histologic scores	756:788	blinded colonic histologic scores	756:788	Inflammation was quantified by serial fecal lipocalin 2 (Lcn-2) and after four weeks by blinded colonic histologic scores and colonic inflammatory cytokine gene expression.
34684567	5	59	theme	metagenomic	906:916	arg1	sequencing					918:927	shotgun metagenomic sequencing	898:927	shotgun metagenomic sequencing	898:927	Microbiota profiles in cecal contents were determined by shotgun metagenomic sequencing.
34684567	4	60	theme	blinded	756:762	arg1	scores					783:788	blinded colonic histologic scores	756:788	blinded colonic histologic scores	756:788	Inflammation was quantified by serial fecal lipocalin 2 (Lcn-2) and after four weeks by blinded colonic histologic scores and colonic inflammatory cytokine gene expression.
34684567	1	61	theme	used	161:164	arg1	polysorbate-80					240:253	polysorbate-80	240:253	polysorbate-80 (P80)	240:259	Commonly used synthetic dietary emulsifiers, including carboxymethylcellulose (CMC) and polysorbate-80 (P80), promote intestinal inflammation.
34684567	1	61	theme	used	161:164	arg1	emulsifiers					184:194	Commonly used synthetic dietary emulsifiers	152:194	Commonly used synthetic dietary emulsifiers	152:194	Commonly used synthetic dietary emulsifiers, including carboxymethylcellulose (CMC) and polysorbate-80 (P80), promote intestinal inflammation.
34684567	1	61	theme	used	161:164	arg1	carboxymethylcellulose					207:228	carboxymethylcellulose	207:228	carboxymethylcellulose (CMC)	207:234	Commonly used synthetic dietary emulsifiers, including carboxymethylcellulose (CMC) and polysorbate-80 (P80), promote intestinal inflammation.
34684567	8	62	theme	intestinal	1272:1281	arg1	microbiota					1283:1292	specific intestinal microbiota	1263:1292	specific intestinal microbiota	1263:1292	The two emulsifiers differentially affected specific intestinal microbiota.
34684567	9	63	theme	Actinobacteria	1466:1479	arg1	abundance					1448:1456	the abundance	1444:1456	the abundance of both Actinobacteria and Proteobacteria	1444:1498	CMC did not impact bacterial composition but significantly decreased Caudoviricetes (bacteriophages), while P80 exposure non-significantly increased the abundance of both Actinobacteria and Proteobacteria.
34684567	5	64	theme	Microbiota	841:850	arg1	profiles					852:859	Microbiota profiles	841:859	Microbiota profiles in cecal contents	841:877	Microbiota profiles in cecal contents were determined by shotgun metagenomic sequencing.
34684567	2	65	theme	inflammatory	388:399	arg1	environment					401:411	an inflammatory environment	385:411	an inflammatory environment using a novel colitis model of ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases	385:570	We compared abilities of CMC vs. P80 to potentiate colitis and impact human microbiota in an inflammatory environment using a novel colitis model of ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases.
34684567	0	66	theme	Humanized	73:81	arg1	Mice					83:86	Humanized Mice	73:86	Humanized Mice with Inflammatory Bowel Disease Microbiota	73:129	The Emulsifier Carboxymethylcellulose Induces More Aggressive Colitis in Humanized Mice with Inflammatory Bowel Disease Microbiota Than Polysorbate-80.
34684567	6	67	theme	CMC	930:932	arg1	treatment					934:942	CMC treatment	930:942	CMC treatment	930:942	CMC treatment significantly increased fecal Lcn-2 levels compared to P80 and water treatment by one week and throughout the experiment.
34684567	4	68	theme	histologic	772:781	arg1	scores					783:788	blinded colonic histologic scores	756:788	blinded colonic histologic scores	756:788	Inflammation was quantified by serial fecal lipocalin 2 (Lcn-2) and after four weeks by blinded colonic histologic scores and colonic inflammatory cytokine gene expression.
34684567	5	69	from	profiles	852:859	arg1	contents					870:877	cecal contents	864:877	cecal contents	864:877	Microbiota profiles in cecal contents were determined by shotgun metagenomic sequencing.
34684567	1	70	theme	dietary	176:182	arg1	polysorbate-80					240:253	polysorbate-80	240:253	polysorbate-80 (P80)	240:259	Commonly used synthetic dietary emulsifiers, including carboxymethylcellulose (CMC) and polysorbate-80 (P80), promote intestinal inflammation.
34684567	1	70	theme	dietary	176:182	arg1	emulsifiers					184:194	Commonly used synthetic dietary emulsifiers	152:194	Commonly used synthetic dietary emulsifiers	152:194	Commonly used synthetic dietary emulsifiers, including carboxymethylcellulose (CMC) and polysorbate-80 (P80), promote intestinal inflammation.
34684567	1	70	theme	dietary	176:182	arg1	carboxymethylcellulose					207:228	carboxymethylcellulose	207:228	carboxymethylcellulose (CMC)	207:234	Commonly used synthetic dietary emulsifiers, including carboxymethylcellulose (CMC) and polysorbate-80 (P80), promote intestinal inflammation.
34684567	11	71	theme	aggressive	1615:1624	arg1	inflammation					1626:1637	more aggressive inflammation	1610:1637	more aggressive inflammation	1610:1637	CMC promotes more aggressive inflammation without changing bacterial composition.
34684567	2	72	theme	active	537:542	arg1	diseases					563:570	active inflammatory bowel diseases	537:570	active inflammatory bowel diseases	537:570	We compared abilities of CMC vs. P80 to potentiate colitis and impact human microbiota in an inflammatory environment using a novel colitis model of ex-germ-free (GF) IL10-/- mice colonized by pooled fecal transplant from three patients with active inflammatory bowel diseases.
34445403	6	0	theme	expression	871:880	arg1	scoring					818:824	histological damage scoring	798:824	histological damage scoring	798:824	Colon tissue was collected for histological damage scoring and quantification of tight junction protein expression.
34445403	6	0	theme	expression	871:880	arg1	quantification					830:843	quantification	830:843	quantification of tight junction protein expression	830:880	Colon tissue was collected for histological damage scoring and quantification of tight junction protein expression.
34445403	2	1	theme	tight	311:315	arg1	junction					317:324	tight junction	311:324	tight junction (TJ) proteins	311:338	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	2	1	theme	tight	311:315	arg1	TJ					327:328	TJ	327:328	TJ	327:328	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	11	2	from	mechanisms	1609:1618	arg1	studies					1647:1653	further studies	1639:1653	further studies	1639:1653	Thus, diosmectites are promising candidates for therapeutic approaches to target intestinal inflammation and to identify possible underlying mechanisms of diosmectites in further studies.
34445403	2	3	from	damage	289:294	arg1	colitis					414:420	dextran sulfate sodium (DSS) colitis	385:420	dextran sulfate sodium (DSS) colitis	385:420	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	3	4	from	day	553:555	arg1	induction					517:525	induction	517:525	induction of acute DSS-colitis from day 8 to 14	517:563	Diosmectite was administered daily to Balb/c mice from day 1 to 7 by oral gavage, followed by induction of acute DSS-colitis from day 8 to 14 ("Control", n = 6; "DSS", n = 10; "FI5pp + DSS", n = 11).
34445403	3	4	from	day	553:555	arg1	DSS-colitis					536:546	acute DSS-colitis	530:546	acute DSS-colitis from day 8 to 14	530:563	Diosmectite was administered daily to Balb/c mice from day 1 to 7 by oral gavage, followed by induction of acute DSS-colitis from day 8 to 14 ("Control", n = 6; "DSS", n = 10; "FI5pp + DSS", n = 11).
34445403	8	5	theme	diosmectite	969:979	arg1	treatment					981:989	prophylactic diosmectite treatment	956:989	prophylactic diosmectite treatment	956:989	Our study revealed prophylactic diosmectite treatment attenuated the severity of DSS colitis, which was apparent by significantly reduced weight loss (p = 0.022 vs. DSS), disease activity index (p = 0.0025 vs. DSS) and histological damage score (p = 0.023 vs. DSS).
34445403	2	6	from	expression	297:306	arg1	colitis					414:420	dextran sulfate sodium (DSS) colitis	385:420	dextran sulfate sodium (DSS) colitis	385:420	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	10	7	theme	amplicon	1382:1389	arg1	NGS					1391:1393	16S amplicon NGS	1378:1393	16S amplicon NGS	1378:1393	Characterization of the microbial composition by 16S amplicon NGS showed that diosmectite treatment modified the DSS-associated dysbiosis.
34445403	7	8	theme	Stool	883:887	arg1	samples					889:895	Stool samples	883:895	Stool samples	883:895	Stool samples were collected for microbiome analysis.
34445403	8	9	dep	0.0025	1136:1141	arg1	DSS					1147:1149	DSS	1147:1149	DSS	1147:1149	Our study revealed prophylactic diosmectite treatment attenuated the severity of DSS colitis, which was apparent by significantly reduced weight loss (p = 0.022 vs. DSS), disease activity index (p = 0.0025 vs. DSS) and histological damage score (p = 0.023 vs. DSS).
34445403	3	10	theme	oral	492:495	arg1	gavage					497:502	oral gavage	492:502	oral gavage	492:502	Diosmectite was administered daily to Balb/c mice from day 1 to 7 by oral gavage, followed by induction of acute DSS-colitis from day 8 to 14 ("Control", n = 6; "DSS", n = 10; "FI5pp + DSS", n = 11).
34445403	1	11	theme	diarrhea	172:179	arg1	treatment					159:167	the treatment	155:167	the treatment of diarrhea	155:179	Natural smectites have demonstrated efficacy in the treatment of diarrhea.
34445403	8	12	theme	reduced	1067:1073	arg1	loss					1082:1085	significantly reduced weight loss	1053:1085	significantly reduced weight loss (p = 0.022 vs. DSS)	1053:1105	Our study revealed prophylactic diosmectite treatment attenuated the severity of DSS colitis, which was apparent by significantly reduced weight loss (p = 0.022 vs. DSS), disease activity index (p = 0.0025 vs. DSS) and histological damage score (p = 0.023 vs. DSS).
34445403	2	13	theme	clinical	266:273	arg1	course					275:280	the clinical course	262:280	the clinical course	262:280	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	8	14	dep	index	1125:1129	arg1	0.0025					1136:1141	p = 0.0025	1132:1141	p = 0.0025	1132:1141	Our study revealed prophylactic diosmectite treatment attenuated the severity of DSS colitis, which was apparent by significantly reduced weight loss (p = 0.022 vs. DSS), disease activity index (p = 0.0025 vs. DSS) and histological damage score (p = 0.023 vs. DSS).
34445403	8	15	theme	DSS	1018:1020	arg1	colitis					1022:1028	DSS colitis	1018:1028	DSS colitis	1018:1028	Our study revealed prophylactic diosmectite treatment attenuated the severity of DSS colitis, which was apparent by significantly reduced weight loss (p = 0.022 vs. DSS), disease activity index (p = 0.0025 vs. DSS) and histological damage score (p = 0.023 vs. DSS).
34445403	10	16	theme	composition	1363:1373	arg1	Characterization					1329:1344	Characterization	1329:1344	Characterization of the microbial composition by 16S amplicon NGS	1329:1393	Characterization of the microbial composition by 16S amplicon NGS showed that diosmectite treatment modified the DSS-associated dysbiosis.
34445403	2	17	theme	prophylactic	214:225	arg1	effect					227:232	the prophylactic effect	210:232	the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis	210:420	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	9	18	theme	significant	1206:1216	arg1	effects					1218:1224	No significant effects	1203:1224	No significant effects	1203:1224	No significant effects were obtained for the expression of TJ proteins (claudin-2 and claudin-3) after diosmectite treatment.
34445403	8	19	theme	p	1088:1088	arg1	0.022					1092:1096	p = 0.022	1088:1096	p = 0.022	1088:1096	Our study revealed prophylactic diosmectite treatment attenuated the severity of DSS colitis, which was apparent by significantly reduced weight loss (p = 0.022 vs. DSS), disease activity index (p = 0.0025 vs. DSS) and histological damage score (p = 0.023 vs. DSS).
34445403	11	20	theme	possible	1589:1596	arg1	mechanisms					1609:1618	possible underlying mechanisms	1589:1618	possible underlying mechanisms of diosmectites in further studies	1589:1653	Thus, diosmectites are promising candidates for therapeutic approaches to target intestinal inflammation and to identify possible underlying mechanisms of diosmectites in further studies.
34445403	11	21	dep	approaches	1528:1537	arg1	identify					1580:1587	identify	1580:1587	to identify possible underlying mechanisms of diosmectites in further studies	1577:1653	Thus, diosmectites are promising candidates for therapeutic approaches to target intestinal inflammation and to identify possible underlying mechanisms of diosmectites in further studies.
34445403	11	21	dep	approaches	1528:1537	arg1	target					1542:1547	target	1542:1547	to target intestinal inflammation	1539:1571	Thus, diosmectites are promising candidates for therapeutic approaches to target intestinal inflammation and to identify possible underlying mechanisms of diosmectites in further studies.
34445403	3	22	theme	DSS-colitis	536:546	arg1	induction					517:525	induction	517:525	induction of acute DSS-colitis from day 8 to 14	517:563	Diosmectite was administered daily to Balb/c mice from day 1 to 7 by oral gavage, followed by induction of acute DSS-colitis from day 8 to 14 ("Control", n = 6; "DSS", n = 10; "FI5pp + DSS", n = 11).
34445403	5	23	theme	stool	687:691	arg1	symptoms					664:671	Clinical symptoms	655:671	Clinical symptoms (body weight, stool consistency and occult blood)	655:721	Clinical symptoms (body weight, stool consistency and occult blood) were checked daily after colitis induction.
34445403	5	23	theme	stool	687:691	arg1	consistency					693:703	stool consistency	687:703	stool consistency	687:703	Clinical symptoms (body weight, stool consistency and occult blood) were checked daily after colitis induction.
34445403	11	24	theme	promising	1491:1499	arg1	diosmectites					1474:1485	diosmectites	1474:1485	diosmectites	1474:1485	Thus, diosmectites are promising candidates for therapeutic approaches to target intestinal inflammation and to identify possible underlying mechanisms of diosmectites in further studies.
34445403	11	24	theme	promising	1491:1499	arg1	candidates					1501:1510	promising candidates	1491:1510	promising candidates for therapeutic approaches to target intestinal inflammation and to identify possible underlying mechanisms of diosmectites in further studies	1491:1653	Thus, diosmectites are promising candidates for therapeutic approaches to target intestinal inflammation and to identify possible underlying mechanisms of diosmectites in further studies.
34445403	0	25	theme	Clinical	50:57	arg1	Symptoms					59:66	Clinical Symptoms	50:66	Clinical Symptoms of DSS Induced Colitis in Balb/c Mice	50:104	Smectite as a Preventive Oral Treatment to Reduce Clinical Symptoms of DSS Induced Colitis in Balb/c Mice.
34445403	2	26	from	course	275:280	arg1	colitis					414:420	dextran sulfate sodium (DSS) colitis	385:420	dextran sulfate sodium (DSS) colitis	385:420	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	5	27	theme	body	674:677	arg1	symptoms					664:671	Clinical symptoms	655:671	Clinical symptoms (body weight, stool consistency and occult blood)	655:721	Clinical symptoms (body weight, stool consistency and occult blood) were checked daily after colitis induction.
34445403	5	27	theme	body	674:677	arg1	weight					679:684	body weight	674:684	body weight	674:684	Clinical symptoms (body weight, stool consistency and occult blood) were checked daily after colitis induction.
34445403	2	28	theme	diosmectite	239:249	arg1	effect					227:232	the prophylactic effect	210:232	the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis	210:420	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	8	29	theme	activity	1116:1123	arg1	index					1125:1129	disease activity index	1108:1129	disease activity index (p = 0.0025 vs. DSS)	1108:1150	Our study revealed prophylactic diosmectite treatment attenuated the severity of DSS colitis, which was apparent by significantly reduced weight loss (p = 0.022 vs. DSS), disease activity index (p = 0.0025 vs. DSS) and histological damage score (p = 0.023 vs. DSS).
34445403	0	30	theme	Induced	75:81	arg1	Colitis					83:89	DSS Induced Colitis	71:89	DSS Induced Colitis	71:89	Smectite as a Preventive Oral Treatment to Reduce Clinical Symptoms of DSS Induced Colitis in Balb/c Mice.
34445403	11	31	theme	therapeutic	1516:1526	arg1	approaches					1528:1537	therapeutic approaches	1516:1537	therapeutic approaches to target intestinal inflammation and to identify possible underlying mechanisms of diosmectites in further studies	1516:1653	Thus, diosmectites are promising candidates for therapeutic approaches to target intestinal inflammation and to identify possible underlying mechanisms of diosmectites in further studies.
34445403	2	32	from	effect	227:232	arg1	expression					297:306	expression	297:306	expression of tight junction (TJ) proteins	297:338	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	2	32	from	effect	227:232	arg1	course					275:280	the clinical course	262:280	the clinical course	262:280	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	2	32	from	effect	227:232	arg1	damage					289:294	colon damage	283:294	colon damage	283:294	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	2	32	from	effect	227:232	arg1	composition					348:358	the composition	344:358	the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis	344:420	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	6	33	theme	tight	848:852	arg1	expression					871:880	tight junction protein expression	848:880	tight junction protein expression	848:880	Colon tissue was collected for histological damage scoring and quantification of tight junction protein expression.
34445403	2	34	theme	sulfate	393:399	arg1	DSS					409:411	DSS	409:411	DSS	409:411	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	2	34	theme	sulfate	393:399	arg1	sodium					401:406	dextran sulfate sodium	385:406	dextran sulfate sodium (DSS) colitis	385:420	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	8	35	theme	=	1134:1134	arg1	0.0025					1136:1141	p = 0.0025	1132:1141	p = 0.0025	1132:1141	Our study revealed prophylactic diosmectite treatment attenuated the severity of DSS colitis, which was apparent by significantly reduced weight loss (p = 0.022 vs. DSS), disease activity index (p = 0.0025 vs. DSS) and histological damage score (p = 0.023 vs. DSS).
34445403	2	36	theme	proteins	331:338	arg1	expression					297:306	expression	297:306	expression of tight junction (TJ) proteins	297:338	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	2	36	theme	proteins	331:338	arg1	course					275:280	the clinical course	262:280	the clinical course	262:280	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	2	36	theme	proteins	331:338	arg1	damage					289:294	colon damage	283:294	colon damage	283:294	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	2	36	theme	proteins	331:338	arg1	composition					348:358	the composition	344:358	the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis	344:420	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	3	37	dep	"	574:574	arg1	"					611:611	DSS"	608:611	DSS"	608:611	Diosmectite was administered daily to Balb/c mice from day 1 to 7 by oral gavage, followed by induction of acute DSS-colitis from day 8 to 14 ("Control", n = 6; "DSS", n = 10; "FI5pp + DSS", n = 11).
34445403	3	37	dep	"	574:574	arg1	n					614:614	n = 11	614:619	n = 11	614:619	Diosmectite was administered daily to Balb/c mice from day 1 to 7 by oral gavage, followed by induction of acute DSS-colitis from day 8 to 14 ("Control", n = 6; "DSS", n = 10; "FI5pp + DSS", n = 11).
34445403	3	37	dep	"	574:574	arg1	FI5pp					600:604	FI5pp	600:604	FI5pp	600:604	Diosmectite was administered daily to Balb/c mice from day 1 to 7 by oral gavage, followed by induction of acute DSS-colitis from day 8 to 14 ("Control", n = 6; "DSS", n = 10; "FI5pp + DSS", n = 11).
34445403	3	37	dep	"	574:574	arg1	n					577:577	n = 6	577:581	n = 6	577:581	Diosmectite was administered daily to Balb/c mice from day 1 to 7 by oral gavage, followed by induction of acute DSS-colitis from day 8 to 14 ("Control", n = 6; "DSS", n = 10; "FI5pp + DSS", n = 11).
34445403	3	37	dep	"	574:574	arg1	"					588:588	"DSS"	584:588	"DSS"	584:588	Diosmectite was administered daily to Balb/c mice from day 1 to 7 by oral gavage, followed by induction of acute DSS-colitis from day 8 to 14 ("Control", n = 6; "DSS", n = 10; "FI5pp + DSS", n = 11).
34445403	3	37	dep	"	574:574	arg1	n					591:591	n	591:591	n = 10	591:596	Diosmectite was administered daily to Balb/c mice from day 1 to 7 by oral gavage, followed by induction of acute DSS-colitis from day 8 to 14 ("Control", n = 6; "DSS", n = 10; "FI5pp + DSS", n = 11).
34445403	5	38	theme	occult	709:714	arg1	blood					716:720	occult blood	709:720	occult blood	709:720	Clinical symptoms (body weight, stool consistency and occult blood) were checked daily after colitis induction.
34445403	5	38	theme	occult	709:714	arg1	symptoms					664:671	Clinical symptoms	655:671	Clinical symptoms (body weight, stool consistency and occult blood)	655:721	Clinical symptoms (body weight, stool consistency and occult blood) were checked daily after colitis induction.
34445403	11	39	from	studies	1647:1653	arg1	mechanisms					1609:1618	possible underlying mechanisms	1589:1618	possible underlying mechanisms of diosmectites in further studies	1589:1653	Thus, diosmectites are promising candidates for therapeutic approaches to target intestinal inflammation and to identify possible underlying mechanisms of diosmectites in further studies.
34445403	2	40	theme	gut	367:369	arg1	microbiota					371:380	the gut microbiota	363:380	the gut microbiota	363:380	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	9	41	theme	diosmectite	1306:1316	arg1	treatment					1318:1326	diosmectite treatment	1306:1326	diosmectite treatment	1306:1326	No significant effects were obtained for the expression of TJ proteins (claudin-2 and claudin-3) after diosmectite treatment.
34445403	1	42	theme	Natural	107:113	arg1	smectites					115:123	Natural smectites	107:123	Natural smectites	107:123	Natural smectites have demonstrated efficacy in the treatment of diarrhea.
34445403	10	43	theme	diosmectite	1407:1417	arg1	treatment					1419:1427	diosmectite treatment	1407:1427	diosmectite treatment	1407:1427	Characterization of the microbial composition by 16S amplicon NGS showed that diosmectite treatment modified the DSS-associated dysbiosis.
34445403	0	44	theme	Oral	25:28	arg1	Treatment					30:38	a Preventive Oral Treatment	12:38	a Preventive Oral Treatment to Reduce Clinical Symptoms of DSS Induced Colitis in Balb/c Mice	12:104	Smectite as a Preventive Oral Treatment to Reduce Clinical Symptoms of DSS Induced Colitis in Balb/c Mice.
34445403	6	45	theme	histological	798:809	arg1	scoring					818:824	histological damage scoring	798:824	histological damage scoring	798:824	Colon tissue was collected for histological damage scoring and quantification of tight junction protein expression.
34445403	8	46	theme	histological	1156:1167	arg1	score					1176:1180	histological damage score	1156:1180	histological damage score (p = 0.023 vs. DSS)	1156:1200	Our study revealed prophylactic diosmectite treatment attenuated the severity of DSS colitis, which was apparent by significantly reduced weight loss (p = 0.022 vs. DSS), disease activity index (p = 0.0025 vs. DSS) and histological damage score (p = 0.023 vs. DSS).
34445403	11	47	theme	further	1639:1645	arg1	studies					1647:1653	further studies	1639:1653	further studies	1639:1653	Thus, diosmectites are promising candidates for therapeutic approaches to target intestinal inflammation and to identify possible underlying mechanisms of diosmectites in further studies.
34445403	8	48	dep	attenuated	991:1000	arg1	apparent					1041:1048	apparent	1041:1048	apparent	1041:1048	Our study revealed prophylactic diosmectite treatment attenuated the severity of DSS colitis, which was apparent by significantly reduced weight loss (p = 0.022 vs. DSS), disease activity index (p = 0.0025 vs. DSS) and histological damage score (p = 0.023 vs. DSS).
34445403	3	49	theme	Control	567:573	arg1	"					574:574	"Control"	566:574	"Control"	566:574	Diosmectite was administered daily to Balb/c mice from day 1 to 7 by oral gavage, followed by induction of acute DSS-colitis from day 8 to 14 ("Control", n = 6; "DSS", n = 10; "FI5pp + DSS", n = 11).
34445403	10	50	theme	DSS-associated	1442:1455	arg1	dysbiosis					1457:1465	the DSS-associated dysbiosis	1438:1465	the DSS-associated dysbiosis	1438:1465	Characterization of the microbial composition by 16S amplicon NGS showed that diosmectite treatment modified the DSS-associated dysbiosis.
34445403	3	51	theme	=	579:579	arg1	n					577:577	n = 6	577:581	n = 6	577:581	Diosmectite was administered daily to Balb/c mice from day 1 to 7 by oral gavage, followed by induction of acute DSS-colitis from day 8 to 14 ("Control", n = 6; "DSS", n = 10; "FI5pp + DSS", n = 11).
34445403	2	52	theme	junction	317:324	arg1	proteins					331:338	tight junction (TJ) proteins	311:338	tight junction (TJ) proteins	311:338	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	6	53	theme	protein	863:869	arg1	expression					871:880	tight junction protein expression	848:880	tight junction protein expression	848:880	Colon tissue was collected for histological damage scoring and quantification of tight junction protein expression.
34445403	3	54	theme	DSS	585:587	arg1	"					588:588	"DSS"	584:588	"DSS"	584:588	Diosmectite was administered daily to Balb/c mice from day 1 to 7 by oral gavage, followed by induction of acute DSS-colitis from day 8 to 14 ("Control", n = 6; "DSS", n = 10; "FI5pp + DSS", n = 11).
34445403	8	55	theme	prophylactic	956:967	arg1	treatment					981:989	prophylactic diosmectite treatment	956:989	prophylactic diosmectite treatment	956:989	Our study revealed prophylactic diosmectite treatment attenuated the severity of DSS colitis, which was apparent by significantly reduced weight loss (p = 0.022 vs. DSS), disease activity index (p = 0.0025 vs. DSS) and histological damage score (p = 0.023 vs. DSS).
34445403	2	56	theme	colon	283:287	arg1	damage					289:294	colon damage	283:294	colon damage	283:294	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	3	57	dep	followed	505:512	arg1	"					574:574	"Control"	566:574	"Control"	566:574	Diosmectite was administered daily to Balb/c mice from day 1 to 7 by oral gavage, followed by induction of acute DSS-colitis from day 8 to 14 ("Control", n = 6; "DSS", n = 10; "FI5pp + DSS", n = 11).
34445403	3	58	theme	=	593:593	arg1	n					591:591	n	591:591	n = 10	591:596	Diosmectite was administered daily to Balb/c mice from day 1 to 7 by oral gavage, followed by induction of acute DSS-colitis from day 8 to 14 ("Control", n = 6; "DSS", n = 10; "FI5pp + DSS", n = 11).
34445403	9	59	theme	TJ	1262:1263	arg1	proteins					1265:1272	TJ proteins	1262:1272	TJ proteins	1262:1272	No significant effects were obtained for the expression of TJ proteins (claudin-2 and claudin-3) after diosmectite treatment.
34445403	0	60	from	Symptoms	59:66	arg1	Mice					101:104	Balb/c Mice	94:104	Balb/c Mice	94:104	Smectite as a Preventive Oral Treatment to Reduce Clinical Symptoms of DSS Induced Colitis in Balb/c Mice.
34445403	8	61	theme	weight	1075:1080	arg1	loss					1082:1085	significantly reduced weight loss	1053:1085	significantly reduced weight loss (p = 0.022 vs. DSS)	1053:1105	Our study revealed prophylactic diosmectite treatment attenuated the severity of DSS colitis, which was apparent by significantly reduced weight loss (p = 0.022 vs. DSS), disease activity index (p = 0.0025 vs. DSS) and histological damage score (p = 0.023 vs. DSS).
34445403	10	62	theme	microbial	1353:1361	arg1	composition					1363:1373	the microbial composition	1349:1373	the microbial composition	1349:1373	Characterization of the microbial composition by 16S amplicon NGS showed that diosmectite treatment modified the DSS-associated dysbiosis.
34445403	8	63	theme	colitis	1022:1028	arg1	severity					1006:1013	the severity	1002:1013	the severity of DSS colitis	1002:1028	Our study revealed prophylactic diosmectite treatment attenuated the severity of DSS colitis, which was apparent by significantly reduced weight loss (p = 0.022 vs. DSS), disease activity index (p = 0.0025 vs. DSS) and histological damage score (p = 0.023 vs. DSS).
34445403	2	64	theme	present	186:192	arg1	study					194:198	The present study	182:198	The present study	182:198	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	11	65	theme	underlying	1598:1607	arg1	mechanisms					1609:1618	possible underlying mechanisms	1589:1618	possible underlying mechanisms of diosmectites in further studies	1589:1653	Thus, diosmectites are promising candidates for therapeutic approaches to target intestinal inflammation and to identify possible underlying mechanisms of diosmectites in further studies.
34445403	8	66	dep	0.022	1092:1096	arg1	DSS					1102:1104	DSS	1102:1104	DSS	1102:1104	Our study revealed prophylactic diosmectite treatment attenuated the severity of DSS colitis, which was apparent by significantly reduced weight loss (p = 0.022 vs. DSS), disease activity index (p = 0.0025 vs. DSS) and histological damage score (p = 0.023 vs. DSS).
34445403	3	67	theme	acute	530:534	arg1	DSS-colitis					536:546	acute DSS-colitis	530:546	acute DSS-colitis from day 8 to 14	530:563	Diosmectite was administered daily to Balb/c mice from day 1 to 7 by oral gavage, followed by induction of acute DSS-colitis from day 8 to 14 ("Control", n = 6; "DSS", n = 10; "FI5pp + DSS", n = 11).
34445403	8	68	dep	score	1176:1180	arg1	DSS					1197:1199	DSS	1197:1199	DSS	1197:1199	Our study revealed prophylactic diosmectite treatment attenuated the severity of DSS colitis, which was apparent by significantly reduced weight loss (p = 0.022 vs. DSS), disease activity index (p = 0.0025 vs. DSS) and histological damage score (p = 0.023 vs. DSS).
34445403	8	68	dep	score	1176:1180	arg1	p					1183:1183	p	1183:1183	p = 0.023	1183:1191	Our study revealed prophylactic diosmectite treatment attenuated the severity of DSS colitis, which was apparent by significantly reduced weight loss (p = 0.022 vs. DSS), disease activity index (p = 0.0025 vs. DSS) and histological damage score (p = 0.023 vs. DSS).
34445403	9	69	dep	obtained	1231:1238	arg1	claudin-2					1275:1283	claudin-2	1275:1283	claudin-2	1275:1283	No significant effects were obtained for the expression of TJ proteins (claudin-2 and claudin-3) after diosmectite treatment.
34445403	9	69	dep	obtained	1231:1238	arg1	claudin-3					1289:1297	claudin-3	1289:1297	claudin-3	1289:1297	No significant effects were obtained for the expression of TJ proteins (claudin-2 and claudin-3) after diosmectite treatment.
34445403	8	70	theme	=	1090:1090	arg1	0.022					1092:1096	p = 0.022	1088:1096	p = 0.022	1088:1096	Our study revealed prophylactic diosmectite treatment attenuated the severity of DSS colitis, which was apparent by significantly reduced weight loss (p = 0.022 vs. DSS), disease activity index (p = 0.0025 vs. DSS) and histological damage score (p = 0.023 vs. DSS).
34445403	11	71	theme	diosmectites	1623:1634	arg1	mechanisms					1609:1618	possible underlying mechanisms	1589:1618	possible underlying mechanisms of diosmectites in further studies	1589:1653	Thus, diosmectites are promising candidates for therapeutic approaches to target intestinal inflammation and to identify possible underlying mechanisms of diosmectites in further studies.
34445403	5	72	theme	Clinical	655:662	arg1	blood					716:720	occult blood	709:720	occult blood	709:720	Clinical symptoms (body weight, stool consistency and occult blood) were checked daily after colitis induction.
34445403	5	72	theme	Clinical	655:662	arg1	symptoms					664:671	Clinical symptoms	655:671	Clinical symptoms (body weight, stool consistency and occult blood)	655:721	Clinical symptoms (body weight, stool consistency and occult blood) were checked daily after colitis induction.
34445403	5	72	theme	Clinical	655:662	arg1	weight					679:684	body weight	674:684	body weight	674:684	Clinical symptoms (body weight, stool consistency and occult blood) were checked daily after colitis induction.
34445403	5	72	theme	Clinical	655:662	arg1	consistency					693:703	stool consistency	687:703	stool consistency	687:703	Clinical symptoms (body weight, stool consistency and occult blood) were checked daily after colitis induction.
34445403	3	73	theme	DSS	608:610	arg1	"					611:611	DSS"	608:611	DSS"	608:611	Diosmectite was administered daily to Balb/c mice from day 1 to 7 by oral gavage, followed by induction of acute DSS-colitis from day 8 to 14 ("Control", n = 6; "DSS", n = 10; "FI5pp + DSS", n = 11).
34445403	0	74	theme	DSS	71:73	arg1	Colitis					83:89	DSS Induced Colitis	71:89	DSS Induced Colitis	71:89	Smectite as a Preventive Oral Treatment to Reduce Clinical Symptoms of DSS Induced Colitis in Balb/c Mice.
34445403	6	75	theme	Colon	767:771	arg1	tissue					773:778	Colon tissue	767:778	Colon tissue	767:778	Colon tissue was collected for histological damage scoring and quantification of tight junction protein expression.
34445403	3	76	from	day	478:480	arg1	mice					468:471	Balb/c mice	461:471	Balb/c mice from day 1 to 7	461:487	Diosmectite was administered daily to Balb/c mice from day 1 to 7 by oral gavage, followed by induction of acute DSS-colitis from day 8 to 14 ("Control", n = 6; "DSS", n = 10; "FI5pp + DSS", n = 11).
34445403	11	77	theme	intestinal	1549:1558	arg1	inflammation					1560:1571	intestinal inflammation	1549:1571	intestinal inflammation	1549:1571	Thus, diosmectites are promising candidates for therapeutic approaches to target intestinal inflammation and to identify possible underlying mechanisms of diosmectites in further studies.
34445403	0	78	theme	Colitis	83:89	arg1	Symptoms					59:66	Clinical Symptoms	50:66	Clinical Symptoms of DSS Induced Colitis in Balb/c Mice	50:104	Smectite as a Preventive Oral Treatment to Reduce Clinical Symptoms of DSS Induced Colitis in Balb/c Mice.
34445403	3	79	dep	=	593:593	arg1	10					595:596	10	595:596	10	595:596	Diosmectite was administered daily to Balb/c mice from day 1 to 7 by oral gavage, followed by induction of acute DSS-colitis from day 8 to 14 ("Control", n = 6; "DSS", n = 10; "FI5pp + DSS", n = 11).
34445403	9	80	theme	proteins	1265:1272	arg1	expression					1248:1257	the expression	1244:1257	the expression of TJ proteins	1244:1272	No significant effects were obtained for the expression of TJ proteins (claudin-2 and claudin-3) after diosmectite treatment.
34445403	8	81	theme	disease	1108:1114	arg1	index					1125:1129	disease activity index	1108:1129	disease activity index (p = 0.0025 vs. DSS)	1108:1150	Our study revealed prophylactic diosmectite treatment attenuated the severity of DSS colitis, which was apparent by significantly reduced weight loss (p = 0.022 vs. DSS), disease activity index (p = 0.0025 vs. DSS) and histological damage score (p = 0.023 vs. DSS).
34445403	3	82	from	induction	517:525	arg1	day					553:555	day 8 to 14	553:563	day 8 to 14	553:563	Diosmectite was administered daily to Balb/c mice from day 1 to 7 by oral gavage, followed by induction of acute DSS-colitis from day 8 to 14 ("Control", n = 6; "DSS", n = 10; "FI5pp + DSS", n = 11).
34445403	3	83	theme	=	616:616	arg1	n					614:614	n = 11	614:619	n = 11	614:619	Diosmectite was administered daily to Balb/c mice from day 1 to 7 by oral gavage, followed by induction of acute DSS-colitis from day 8 to 14 ("Control", n = 6; "DSS", n = 10; "FI5pp + DSS", n = 11).
34445403	0	84	theme	Balb/c	94:99	arg1	Mice					101:104	Balb/c Mice	94:104	Balb/c Mice	94:104	Smectite as a Preventive Oral Treatment to Reduce Clinical Symptoms of DSS Induced Colitis in Balb/c Mice.
34445403	6	85	theme	junction	854:861	arg1	expression					871:880	tight junction protein expression	848:880	tight junction protein expression	848:880	Colon tissue was collected for histological damage scoring and quantification of tight junction protein expression.
34445403	11	86	from	diosmectites	1623:1634	arg1	studies					1647:1653	further studies	1639:1653	further studies	1639:1653	Thus, diosmectites are promising candidates for therapeutic approaches to target intestinal inflammation and to identify possible underlying mechanisms of diosmectites in further studies.
34445403	2	87	theme	dextran	385:391	arg1	DSS					409:411	DSS	409:411	DSS	409:411	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	2	87	theme	dextran	385:391	arg1	sodium					401:406	dextran sulfate sodium	385:406	dextran sulfate sodium (DSS) colitis	385:420	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	8	88	theme	p	1132:1132	arg1	0.0025					1136:1141	p = 0.0025	1132:1141	p = 0.0025	1132:1141	Our study revealed prophylactic diosmectite treatment attenuated the severity of DSS colitis, which was apparent by significantly reduced weight loss (p = 0.022 vs. DSS), disease activity index (p = 0.0025 vs. DSS) and histological damage score (p = 0.023 vs. DSS).
34445403	10	89	theme	16S	1378:1380	arg1	NGS					1391:1393	16S amplicon NGS	1378:1393	16S amplicon NGS	1378:1393	Characterization of the microbial composition by 16S amplicon NGS showed that diosmectite treatment modified the DSS-associated dysbiosis.
34445403	0	90	theme	Preventive	14:23	arg1	Treatment					30:38	a Preventive Oral Treatment	12:38	a Preventive Oral Treatment to Reduce Clinical Symptoms of DSS Induced Colitis in Balb/c Mice	12:104	Smectite as a Preventive Oral Treatment to Reduce Clinical Symptoms of DSS Induced Colitis in Balb/c Mice.
34445403	2	91	theme	microbiota	371:380	arg1	expression					297:306	expression	297:306	expression of tight junction (TJ) proteins	297:338	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	2	91	theme	microbiota	371:380	arg1	course					275:280	the clinical course	262:280	the clinical course	262:280	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	2	91	theme	microbiota	371:380	arg1	damage					289:294	colon damage	283:294	colon damage	283:294	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	2	91	theme	microbiota	371:380	arg1	composition					348:358	the composition	344:358	the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis	344:420	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	3	92	dep	day	478:480	arg1	to					484:485	to	484:485	to	484:485	Diosmectite was administered daily to Balb/c mice from day 1 to 7 by oral gavage, followed by induction of acute DSS-colitis from day 8 to 14 ("Control", n = 6; "DSS", n = 10; "FI5pp + DSS", n = 11).
34445403	5	93	dep	symptoms	664:671	arg1	blood					716:720	occult blood	709:720	occult blood	709:720	Clinical symptoms (body weight, stool consistency and occult blood) were checked daily after colitis induction.
34445403	5	93	dep	symptoms	664:671	arg1	symptoms					664:671	Clinical symptoms	655:671	Clinical symptoms (body weight, stool consistency and occult blood)	655:721	Clinical symptoms (body weight, stool consistency and occult blood) were checked daily after colitis induction.
34445403	5	93	dep	symptoms	664:671	arg1	weight					679:684	body weight	674:684	body weight	674:684	Clinical symptoms (body weight, stool consistency and occult blood) were checked daily after colitis induction.
34445403	5	93	dep	symptoms	664:671	arg1	consistency					693:703	stool consistency	687:703	stool consistency	687:703	Clinical symptoms (body weight, stool consistency and occult blood) were checked daily after colitis induction.
34445403	6	94	theme	damage	811:816	arg1	scoring					818:824	histological damage scoring	798:824	histological damage scoring	798:824	Colon tissue was collected for histological damage scoring and quantification of tight junction protein expression.
34445403	7	95	theme	microbiome	916:925	arg1	analysis					927:934	microbiome analysis	916:934	microbiome analysis	916:934	Stool samples were collected for microbiome analysis.
34445403	2	96	theme	sodium	401:406	arg1	colitis					414:420	dextran sulfate sodium (DSS) colitis	385:420	dextran sulfate sodium (DSS) colitis	385:420	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	5	97	theme	colitis	748:754	arg1	induction					756:764	colitis induction	748:764	colitis induction	748:764	Clinical symptoms (body weight, stool consistency and occult blood) were checked daily after colitis induction.
34445403	8	98	theme	damage	1169:1174	arg1	score					1176:1180	histological damage score	1156:1180	histological damage score (p = 0.023 vs. DSS)	1156:1200	Our study revealed prophylactic diosmectite treatment attenuated the severity of DSS colitis, which was apparent by significantly reduced weight loss (p = 0.022 vs. DSS), disease activity index (p = 0.0025 vs. DSS) and histological damage score (p = 0.023 vs. DSS).
34445403	8	99	dep	loss	1082:1085	arg1	0.022					1092:1096	p = 0.022	1088:1096	p = 0.022	1088:1096	Our study revealed prophylactic diosmectite treatment attenuated the severity of DSS colitis, which was apparent by significantly reduced weight loss (p = 0.022 vs. DSS), disease activity index (p = 0.0025 vs. DSS) and histological damage score (p = 0.023 vs. DSS).
34445403	3	100	dep	day	553:555	arg1	to					559:560	to	559:560	to	559:560	Diosmectite was administered daily to Balb/c mice from day 1 to 7 by oral gavage, followed by induction of acute DSS-colitis from day 8 to 14 ("Control", n = 6; "DSS", n = 10; "FI5pp + DSS", n = 11).
34445403	2	101	from	composition	348:358	arg1	colitis					414:420	dextran sulfate sodium (DSS) colitis	385:420	dextran sulfate sodium (DSS) colitis	385:420	The present study evaluated the prophylactic effect of a diosmectite (FI5pp) on the clinical course, colon damage, expression of tight junction (TJ) proteins and the composition of the gut microbiota in dextran sulfate sodium (DSS) colitis.
34445403	3	102	theme	Balb/c	461:466	arg1	mice					468:471	Balb/c mice	461:471	Balb/c mice from day 1 to 7	461:487	Diosmectite was administered daily to Balb/c mice from day 1 to 7 by oral gavage, followed by induction of acute DSS-colitis from day 8 to 14 ("Control", n = 6; "DSS", n = 10; "FI5pp + DSS", n = 11).
33301522	4	0	theme	enrichment	986:995	arg1	materials					997:1005	different enrichment materials	976:1005	different enrichment materials	976:1005	Here, we optimized the sample preparation workflow prior to LC-MS/MS analysis by systematically comparing different analytical methods, including the use of different sources of trypsin, combinations of different proteases, and different enrichment materials.
33301522	1	1	gly	N-glycopeptide	181:194	arg2	N-glycopeptide					181:194	Native intact N-glycopeptide analysis	167:203	Native intact N-glycopeptide analysis	167:203	Native intact N-glycopeptide analysis can provide access to the comprehensive characteristics of N-glycan occupancy, including N-glycosites, N-glycan compositions, and N-glycoproteins for complex samples.
33301522	8	2	theme	different	1791:1799	arg1	conditions					1808:1817	different growth conditions	1791:1817	different growth conditions	1791:1817	This study also provides an interesting comparison of the N-glycoproteome of breast cancer cells cultured in different growth conditions, indicating the important role of N-glycosylated proteins in cancer cell growth and the choice of the cell culture model for studies in tumor biology and drug evaluation.
33301522	8	3	theme	proteins	1868:1875	arg1	choice					1907:1912	the choice	1903:1912	the choice of the cell culture model for studies in tumor biology and drug evaluation	1903:1987	This study also provides an interesting comparison of the N-glycoproteome of breast cancer cells cultured in different growth conditions, indicating the important role of N-glycosylated proteins in cancer cell growth and the choice of the cell culture model for studies in tumor biology and drug evaluation.
33301522	8	3	theme	proteins	1868:1875	arg1	role					1845:1848	the important role	1831:1848	the important role of N-glycosylated proteins in cancer cell growth	1831:1897	This study also provides an interesting comparison of the N-glycoproteome of breast cancer cells cultured in different growth conditions, indicating the important role of N-glycosylated proteins in cancer cell growth and the choice of the cell culture model for studies in tumor biology and drug evaluation.
33301522	1	4	theme	N-glycan	308:315	arg1	compositions					317:328	N-glycan compositions	308:328	N-glycan compositions	308:328	Native intact N-glycopeptide analysis can provide access to the comprehensive characteristics of N-glycan occupancy, including N-glycosites, N-glycan compositions, and N-glycoproteins for complex samples.
33301522	6	5	theme	optimized	1338:1346	arg1	workflow					1348:1355	the optimized workflow	1334:1355	the optimized workflow	1334:1355	Furthermore, the optimized workflow was applied to the analysis of intact N-glycopeptides in two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells in vitro and xenografted tumors in mice.
33301522	3	6	theme	identified	630:639	arg1	N-glycopeptides					648:662	identified intact N-glycopeptides	630:662	identified intact N-glycopeptides	630:662	However, the number of identified intact N-glycopeptides remains inadequate to achieve an in-depth profile of the N-glycosylation landscape.
33301522	8	7	theme	cancer	1880:1885	arg1	growth					1892:1897	cancer cell growth	1880:1897	cancer cell growth	1880:1897	This study also provides an interesting comparison of the N-glycoproteome of breast cancer cells cultured in different growth conditions, indicating the important role of N-glycosylated proteins in cancer cell growth and the choice of the cell culture model for studies in tumor biology and drug evaluation.
33301522	6	8	dep	three-dimensional	1439:1455	arg1	3D					1458:1459	3D	1458:1459	3D	1458:1459	Furthermore, the optimized workflow was applied to the analysis of intact N-glycopeptides in two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells in vitro and xenografted tumors in mice.
33301522	4	9	theme	proteases	961:969	arg1	sources					915:921	different sources	905:921	different sources of trypsin	905:932	Here, we optimized the sample preparation workflow prior to LC-MS/MS analysis by systematically comparing different analytical methods, including the use of different sources of trypsin, combinations of different proteases, and different enrichment materials.
33301522	4	9	theme	proteases	961:969	arg1	materials					997:1005	different enrichment materials	976:1005	different enrichment materials	976:1005	Here, we optimized the sample preparation workflow prior to LC-MS/MS analysis by systematically comparing different analytical methods, including the use of different sources of trypsin, combinations of different proteases, and different enrichment materials.
33301522	4	9	theme	proteases	961:969	arg1	combinations					935:946	combinations	935:946	combinations of different proteases	935:969	Here, we optimized the sample preparation workflow prior to LC-MS/MS analysis by systematically comparing different analytical methods, including the use of different sources of trypsin, combinations of different proteases, and different enrichment materials.
33301522	7	10	theme	same	1565:1568	arg1	cells					1584:1588	the same breast cancer cells	1561:1588	the same breast cancer cells	1561:1588	These results indicated that the same breast cancer cells, when cultured in different microenvironments, can show different N-glycosylation patterns.
33301522	5	11	dep	improved	1138:1145	arg1	offering					1277:1284	offering	1277:1284	offering better analytical reproducibility	1277:1318	Finally, we found that the combination of Trypsin (B)/Lys-C digestion and zwitterionic HILIC (Zic-HILIC) enrichment significantly improved the mass spectrometric characterization of intact N-glycopeptides, increasing the number of identified intact N-glycopeptides and offering better analytical reproducibility.
33301522	5	11	dep	improved	1138:1145	arg1	increasing					1214:1223	increasing	1214:1223	increasing the number of identified intact N-glycopeptides	1214:1271	Finally, we found that the combination of Trypsin (B)/Lys-C digestion and zwitterionic HILIC (Zic-HILIC) enrichment significantly improved the mass spectrometric characterization of intact N-glycopeptides, increasing the number of identified intact N-glycopeptides and offering better analytical reproducibility.
33301522	6	12	theme	two-dimensional	1414:1428	arg1	cells					1485:1489	two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells	1414:1489	two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells	1414:1489	Furthermore, the optimized workflow was applied to the analysis of intact N-glycopeptides in two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells in vitro and xenografted tumors in mice.
33301522	6	13	from	cells	1485:1489	arg1	analysis					1376:1383	the analysis	1372:1383	the analysis of intact N-glycopeptides in two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells in vitro	1372:1498	Furthermore, the optimized workflow was applied to the analysis of intact N-glycopeptides in two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells in vitro and xenografted tumors in mice.
33301522	6	13	from	cells	1485:1489	arg1	tumors					1516:1521	xenografted tumors	1504:1521	xenografted tumors in mice	1504:1529	Furthermore, the optimized workflow was applied to the analysis of intact N-glycopeptides in two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells in vitro and xenografted tumors in mice.
33301522	6	14	from	N-glycopeptides	1395:1409	arg1	cells					1485:1489	two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells	1414:1489	two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells	1414:1489	Furthermore, the optimized workflow was applied to the analysis of intact N-glycopeptides in two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells in vitro and xenografted tumors in mice.
33301522	4	15	theme	sample	771:776	arg1	preparation					778:788	the sample preparation	767:788	the sample preparation workflow prior to LC-MS/MS analysis	767:824	Here, we optimized the sample preparation workflow prior to LC-MS/MS analysis by systematically comparing different analytical methods, including the use of different sources of trypsin, combinations of different proteases, and different enrichment materials.
33301522	6	16	theme	N-glycopeptides	1395:1409	arg1	analysis					1376:1383	the analysis	1372:1383	the analysis of intact N-glycopeptides in two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells in vitro	1372:1498	Furthermore, the optimized workflow was applied to the analysis of intact N-glycopeptides in two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells in vitro and xenografted tumors in mice.
33301522	6	16	theme	N-glycopeptides	1395:1409	arg1	tumors					1516:1521	xenografted tumors	1504:1521	xenografted tumors in mice	1504:1529	Furthermore, the optimized workflow was applied to the analysis of intact N-glycopeptides in two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells in vitro and xenografted tumors in mice.
33301522	0	17	theme	xenografted	147:157	arg1	tumors					159:164	xenografted tumors	147:164	xenografted tumors	147:164	In-depth characterization and comparison of the N-glycosylated proteome of two-dimensional- and three-dimensional-cultured breast cancer cells and xenografted tumors.
33301522	5	18	theme	intact	1190:1195	arg1	N-glycopeptides					1197:1211	intact N-glycopeptides	1190:1211	intact N-glycopeptides	1190:1211	Finally, we found that the combination of Trypsin (B)/Lys-C digestion and zwitterionic HILIC (Zic-HILIC) enrichment significantly improved the mass spectrometric characterization of intact N-glycopeptides, increasing the number of identified intact N-glycopeptides and offering better analytical reproducibility.
33301522	3	19	theme	N-glycopeptides	648:662	arg1	number					620:625	the number	616:625	the number of identified intact N-glycopeptides	616:662	However, the number of identified intact N-glycopeptides remains inadequate to achieve an in-depth profile of the N-glycosylation landscape.
33301522	6	20	theme	intact	1388:1393	arg1	N-glycopeptides					1395:1409	intact N-glycopeptides	1388:1409	intact N-glycopeptides in two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells	1388:1489	Furthermore, the optimized workflow was applied to the analysis of intact N-glycopeptides in two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells in vitro and xenografted tumors in mice.
33301522	8	21	from	studies	1944:1950	arg1	biology					1961:1967	tumor biology	1955:1967	tumor biology	1955:1967	This study also provides an interesting comparison of the N-glycoproteome of breast cancer cells cultured in different growth conditions, indicating the important role of N-glycosylated proteins in cancer cell growth and the choice of the cell culture model for studies in tumor biology and drug evaluation.
33301522	8	21	from	studies	1944:1950	arg1	evaluation					1978:1987	drug evaluation	1973:1987	drug evaluation	1973:1987	This study also provides an interesting comparison of the N-glycoproteome of breast cancer cells cultured in different growth conditions, indicating the important role of N-glycosylated proteins in cancer cell growth and the choice of the cell culture model for studies in tumor biology and drug evaluation.
33301522	6	22	theme	breast	1471:1476	arg1	cells					1485:1489	two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells	1414:1489	two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells	1414:1489	Furthermore, the optimized workflow was applied to the analysis of intact N-glycopeptides in two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells in vitro and xenografted tumors in mice.
33301522	3	23	theme	in-depth	697:704	arg1	profile					706:712	an in-depth profile	694:712	an in-depth profile of the N-glycosylation landscape	694:745	However, the number of identified intact N-glycopeptides remains inadequate to achieve an in-depth profile of the N-glycosylation landscape.
33301522	1	24	gly	N-glycoproteins	335:349	arg1	N-glycoproteins					335:349	N-glycoproteins	335:349	N-glycoproteins	335:349	Native intact N-glycopeptide analysis can provide access to the comprehensive characteristics of N-glycan occupancy, including N-glycosites, N-glycan compositions, and N-glycoproteins for complex samples.
33301522	1	25	theme	N-glycan	264:271	arg1	occupancy					273:281	N-glycan occupancy	264:281	N-glycan occupancy	264:281	Native intact N-glycopeptide analysis can provide access to the comprehensive characteristics of N-glycan occupancy, including N-glycosites, N-glycan compositions, and N-glycoproteins for complex samples.
33301522	5	26	theme	mass	1151:1154	arg1	characterization					1170:1185	the mass spectrometric characterization	1147:1185	the mass spectrometric characterization of intact N-glycopeptides	1147:1211	Finally, we found that the combination of Trypsin (B)/Lys-C digestion and zwitterionic HILIC (Zic-HILIC) enrichment significantly improved the mass spectrometric characterization of intact N-glycopeptides, increasing the number of identified intact N-glycopeptides and offering better analytical reproducibility.
33301522	8	27	gly	N-glycosylated	1853:1866	arg1	proteins					1868:1875	N-glycosylated proteins	1853:1875	N-glycosylated proteins	1853:1875	This study also provides an interesting comparison of the N-glycoproteome of breast cancer cells cultured in different growth conditions, indicating the important role of N-glycosylated proteins in cancer cell growth and the choice of the cell culture model for studies in tumor biology and drug evaluation.
33301522	8	28	theme	model	1934:1938	arg1	choice					1907:1912	the choice	1903:1912	the choice of the cell culture model for studies in tumor biology and drug evaluation	1903:1987	This study also provides an interesting comparison of the N-glycoproteome of breast cancer cells cultured in different growth conditions, indicating the important role of N-glycosylated proteins in cancer cell growth and the choice of the cell culture model for studies in tumor biology and drug evaluation.
33301522	8	28	theme	model	1934:1938	arg1	role					1845:1848	the important role	1831:1848	the important role of N-glycosylated proteins in cancer cell growth	1831:1897	This study also provides an interesting comparison of the N-glycoproteome of breast cancer cells cultured in different growth conditions, indicating the important role of N-glycosylated proteins in cancer cell growth and the choice of the cell culture model for studies in tumor biology and drug evaluation.
33301522	3	29	gly	N-glycopeptides	648:662	arg2	N-glycopeptides					648:662	identified intact N-glycopeptides	630:662	identified intact N-glycopeptides	630:662	However, the number of identified intact N-glycopeptides remains inadequate to achieve an in-depth profile of the N-glycosylation landscape.
33301522	3	30	theme	N-glycosylation	721:735	arg1	landscape					737:745	the N-glycosylation landscape	717:745	the N-glycosylation landscape	717:745	However, the number of identified intact N-glycopeptides remains inadequate to achieve an in-depth profile of the N-glycosylation landscape.
33301522	1	31	theme	Native	167:172	arg1	analysis					196:203	Native intact N-glycopeptide analysis	167:203	Native intact N-glycopeptide analysis	167:203	Native intact N-glycopeptide analysis can provide access to the comprehensive characteristics of N-glycan occupancy, including N-glycosites, N-glycan compositions, and N-glycoproteins for complex samples.
33301522	2	32	theme	LC-MS/MS	587:594	arg1	detection					596:604	LC-MS/MS detection	587:604	LC-MS/MS detection	587:604	The sample pre-processing method used for the analysis of intact N-glycopeptides usually depends on the enrichment of low abundance N-glycopeptides from a tryptic peptide mixture using hydrophilic substances before LC-MS/MS detection.
33301522	4	33	theme	workflow	790:797	arg1	preparation					778:788	the sample preparation	767:788	the sample preparation workflow prior to LC-MS/MS analysis	767:824	Here, we optimized the sample preparation workflow prior to LC-MS/MS analysis by systematically comparing different analytical methods, including the use of different sources of trypsin, combinations of different proteases, and different enrichment materials.
33301522	5	34	theme	intact	1250:1255	arg1	N-glycopeptides					1257:1271	identified intact N-glycopeptides	1239:1271	identified intact N-glycopeptides	1239:1271	Finally, we found that the combination of Trypsin (B)/Lys-C digestion and zwitterionic HILIC (Zic-HILIC) enrichment significantly improved the mass spectrometric characterization of intact N-glycopeptides, increasing the number of identified intact N-glycopeptides and offering better analytical reproducibility.
33301522	8	35	theme	tumor	1955:1959	arg1	biology					1961:1967	tumor biology	1955:1967	tumor biology	1955:1967	This study also provides an interesting comparison of the N-glycoproteome of breast cancer cells cultured in different growth conditions, indicating the important role of N-glycosylated proteins in cancer cell growth and the choice of the cell culture model for studies in tumor biology and drug evaluation.
33301522	1	36	theme	N-glycopeptide	181:194	arg1	analysis					196:203	Native intact N-glycopeptide analysis	167:203	Native intact N-glycopeptide analysis	167:203	Native intact N-glycopeptide analysis can provide access to the comprehensive characteristics of N-glycan occupancy, including N-glycosites, N-glycan compositions, and N-glycoproteins for complex samples.
33301522	8	37	from	role	1845:1848	arg1	growth					1892:1897	cancer cell growth	1880:1897	cancer cell growth	1880:1897	This study also provides an interesting comparison of the N-glycoproteome of breast cancer cells cultured in different growth conditions, indicating the important role of N-glycosylated proteins in cancer cell growth and the choice of the cell culture model for studies in tumor biology and drug evaluation.
33301522	0	38	theme	breast	123:128	arg1	cells					137:141	two-dimensional- and three-dimensional-cultured breast cancer cells	75:141	two-dimensional- and three-dimensional-cultured breast cancer cells	75:141	In-depth characterization and comparison of the N-glycosylated proteome of two-dimensional- and three-dimensional-cultured breast cancer cells and xenografted tumors.
33301522	1	39	gly	N-glycosites	294:305	arg2	N-glycosites					294:305	N-glycosites	294:305	N-glycosites	294:305	Native intact N-glycopeptide analysis can provide access to the comprehensive characteristics of N-glycan occupancy, including N-glycosites, N-glycan compositions, and N-glycoproteins for complex samples.
33301522	5	40	theme	digestion	1068:1076	arg1	combination					1035:1045	the combination	1031:1045	the combination of Trypsin (B)/Lys-C digestion and zwitterionic HILIC (Zic-HILIC) enrichment	1031:1122	Finally, we found that the combination of Trypsin (B)/Lys-C digestion and zwitterionic HILIC (Zic-HILIC) enrichment significantly improved the mass spectrometric characterization of intact N-glycopeptides, increasing the number of identified intact N-glycopeptides and offering better analytical reproducibility.
33301522	0	41	theme	cells	137:141	arg1	proteome					63:70	the N-glycosylated proteome	44:70	the N-glycosylated proteome of two-dimensional- and three-dimensional-cultured breast cancer cells and xenografted tumors	44:164	In-depth characterization and comparison of the N-glycosylated proteome of two-dimensional- and three-dimensional-cultured breast cancer cells and xenografted tumors.
33301522	6	42	from	tumors	1516:1521	arg1	mice					1526:1529	mice	1526:1529	mice	1526:1529	Furthermore, the optimized workflow was applied to the analysis of intact N-glycopeptides in two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells in vitro and xenografted tumors in mice.
33301522	6	42	from	tumors	1516:1521	arg1	cells					1485:1489	two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells	1414:1489	two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells	1414:1489	Furthermore, the optimized workflow was applied to the analysis of intact N-glycopeptides in two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells in vitro and xenografted tumors in mice.
33301522	5	43	theme	zwitterionic	1082:1093	arg1	enrichment					1113:1122	zwitterionic HILIC (Zic-HILIC) enrichment	1082:1122	zwitterionic HILIC (Zic-HILIC) enrichment	1082:1122	Finally, we found that the combination of Trypsin (B)/Lys-C digestion and zwitterionic HILIC (Zic-HILIC) enrichment significantly improved the mass spectrometric characterization of intact N-glycopeptides, increasing the number of identified intact N-glycopeptides and offering better analytical reproducibility.
33301522	2	44	from	mixture	543:549	arg1	enrichment					476:485	the enrichment	472:485	the enrichment of low abundance N-glycopeptides from a tryptic peptide mixture using hydrophilic substances before LC-MS/MS detection	472:604	The sample pre-processing method used for the analysis of intact N-glycopeptides usually depends on the enrichment of low abundance N-glycopeptides from a tryptic peptide mixture using hydrophilic substances before LC-MS/MS detection.
33301522	2	44	from	mixture	543:549	arg1	N-glycopeptides					504:518	low abundance N-glycopeptides	490:518	low abundance N-glycopeptides from a tryptic peptide mixture using hydrophilic substances before LC-MS/MS detection	490:604	The sample pre-processing method used for the analysis of intact N-glycopeptides usually depends on the enrichment of low abundance N-glycopeptides from a tryptic peptide mixture using hydrophilic substances before LC-MS/MS detection.
33301522	2	45	gly	N-glycopeptides	437:451	arg2	N-glycopeptides					437:451	intact N-glycopeptides	430:451	intact N-glycopeptides	430:451	The sample pre-processing method used for the analysis of intact N-glycopeptides usually depends on the enrichment of low abundance N-glycopeptides from a tryptic peptide mixture using hydrophilic substances before LC-MS/MS detection.
33301522	4	46	theme	different	854:862	arg1	methods					875:881	different analytical methods	854:881	different analytical methods	854:881	Here, we optimized the sample preparation workflow prior to LC-MS/MS analysis by systematically comparing different analytical methods, including the use of different sources of trypsin, combinations of different proteases, and different enrichment materials.
33301522	4	46	theme	different	854:862	arg1	use					898:900	the use	894:900	the use of different sources of trypsin, combinations of different proteases, and different enrichment materials	894:1005	Here, we optimized the sample preparation workflow prior to LC-MS/MS analysis by systematically comparing different analytical methods, including the use of different sources of trypsin, combinations of different proteases, and different enrichment materials.
33301522	0	47	theme	In-depth	0:7	arg1	characterization					9:24	In-depth characterization	0:24	In-depth characterization	0:24	In-depth characterization and comparison of the N-glycosylated proteome of two-dimensional- and three-dimensional-cultured breast cancer cells and xenografted tumors.
33301522	2	48	theme	N-glycopeptides	504:518	arg1	enrichment					476:485	the enrichment	472:485	the enrichment of low abundance N-glycopeptides from a tryptic peptide mixture using hydrophilic substances before LC-MS/MS detection	472:604	The sample pre-processing method used for the analysis of intact N-glycopeptides usually depends on the enrichment of low abundance N-glycopeptides from a tryptic peptide mixture using hydrophilic substances before LC-MS/MS detection.
33301522	2	49	theme	low	490:492	arg1	N-glycopeptides					504:518	low abundance N-glycopeptides	490:518	low abundance N-glycopeptides from a tryptic peptide mixture using hydrophilic substances before LC-MS/MS detection	490:604	The sample pre-processing method used for the analysis of intact N-glycopeptides usually depends on the enrichment of low abundance N-glycopeptides from a tryptic peptide mixture using hydrophilic substances before LC-MS/MS detection.
33301522	8	50	theme	interesting	1710:1720	arg1	comparison					1722:1731	an interesting comparison	1707:1731	an interesting comparison of the N-glycoproteome of breast cancer cells cultured in different growth conditions	1707:1817	This study also provides an interesting comparison of the N-glycoproteome of breast cancer cells cultured in different growth conditions, indicating the important role of N-glycosylated proteins in cancer cell growth and the choice of the cell culture model for studies in tumor biology and drug evaluation.
33301522	5	51	theme	better	1286:1291	arg1	reproducibility					1304:1318	better analytical reproducibility	1286:1318	better analytical reproducibility	1286:1318	Finally, we found that the combination of Trypsin (B)/Lys-C digestion and zwitterionic HILIC (Zic-HILIC) enrichment significantly improved the mass spectrometric characterization of intact N-glycopeptides, increasing the number of identified intact N-glycopeptides and offering better analytical reproducibility.
33301522	2	52	theme	peptide	535:541	arg1	mixture					543:549	a tryptic peptide mixture	525:549	a tryptic peptide mixture using hydrophilic substances before LC-MS/MS detection	525:604	The sample pre-processing method used for the analysis of intact N-glycopeptides usually depends on the enrichment of low abundance N-glycopeptides from a tryptic peptide mixture using hydrophilic substances before LC-MS/MS detection.
33301522	0	53	theme	N-glycosylated	48:61	arg1	proteome					63:70	the N-glycosylated proteome	44:70	the N-glycosylated proteome of two-dimensional- and three-dimensional-cultured breast cancer cells and xenografted tumors	44:164	In-depth characterization and comparison of the N-glycosylated proteome of two-dimensional- and three-dimensional-cultured breast cancer cells and xenografted tumors.
33301522	7	54	theme	N-glycosylation	1656:1670	arg1	patterns					1672:1679	different N-glycosylation patterns	1646:1679	different N-glycosylation patterns	1646:1679	These results indicated that the same breast cancer cells, when cultured in different microenvironments, can show different N-glycosylation patterns.
33301522	5	55	gly	N-glycopeptides	1197:1211	arg2	N-glycopeptides					1197:1211	intact N-glycopeptides	1190:1211	intact N-glycopeptides	1190:1211	Finally, we found that the combination of Trypsin (B)/Lys-C digestion and zwitterionic HILIC (Zic-HILIC) enrichment significantly improved the mass spectrometric characterization of intact N-glycopeptides, increasing the number of identified intact N-glycopeptides and offering better analytical reproducibility.
33301522	8	56	theme	N-glycoproteome	1740:1754	arg1	comparison					1722:1731	an interesting comparison	1707:1731	an interesting comparison of the N-glycoproteome of breast cancer cells cultured in different growth conditions	1707:1817	This study also provides an interesting comparison of the N-glycoproteome of breast cancer cells cultured in different growth conditions, indicating the important role of N-glycosylated proteins in cancer cell growth and the choice of the cell culture model for studies in tumor biology and drug evaluation.
33301522	4	57	theme	different	905:913	arg1	sources					915:921	different sources	905:921	different sources of trypsin	905:932	Here, we optimized the sample preparation workflow prior to LC-MS/MS analysis by systematically comparing different analytical methods, including the use of different sources of trypsin, combinations of different proteases, and different enrichment materials.
33301522	1	58	theme	occupancy	273:281	arg1	characteristics					245:259	the comprehensive characteristics	227:259	the comprehensive characteristics	227:259	Native intact N-glycopeptide analysis can provide access to the comprehensive characteristics of N-glycan occupancy, including N-glycosites, N-glycan compositions, and N-glycoproteins for complex samples.
33301522	1	58	theme	occupancy	273:281	arg1	N-glycosites					294:305	N-glycosites	294:305	N-glycosites	294:305	Native intact N-glycopeptide analysis can provide access to the comprehensive characteristics of N-glycan occupancy, including N-glycosites, N-glycan compositions, and N-glycoproteins for complex samples.
33301522	1	58	theme	occupancy	273:281	arg1	compositions					317:328	N-glycan compositions	308:328	N-glycan compositions	308:328	Native intact N-glycopeptide analysis can provide access to the comprehensive characteristics of N-glycan occupancy, including N-glycosites, N-glycan compositions, and N-glycoproteins for complex samples.
33301522	1	58	theme	occupancy	273:281	arg1	N-glycoproteins					335:349	N-glycoproteins	335:349	N-glycoproteins	335:349	Native intact N-glycopeptide analysis can provide access to the comprehensive characteristics of N-glycan occupancy, including N-glycosites, N-glycan compositions, and N-glycoproteins for complex samples.
33301522	2	59	from	enrichment	476:485	arg1	mixture					543:549	a tryptic peptide mixture	525:549	a tryptic peptide mixture using hydrophilic substances before LC-MS/MS detection	525:604	The sample pre-processing method used for the analysis of intact N-glycopeptides usually depends on the enrichment of low abundance N-glycopeptides from a tryptic peptide mixture using hydrophilic substances before LC-MS/MS detection.
33301522	8	60	theme	breast	1759:1764	arg1	cells					1773:1777	breast cancer cells	1759:1777	breast cancer cells cultured in different growth conditions	1759:1817	This study also provides an interesting comparison of the N-glycoproteome of breast cancer cells cultured in different growth conditions, indicating the important role of N-glycosylated proteins in cancer cell growth and the choice of the cell culture model for studies in tumor biology and drug evaluation.
33301522	2	61	theme	intact	430:435	arg1	N-glycopeptides					437:451	intact N-glycopeptides	430:451	intact N-glycopeptides	430:451	The sample pre-processing method used for the analysis of intact N-glycopeptides usually depends on the enrichment of low abundance N-glycopeptides from a tryptic peptide mixture using hydrophilic substances before LC-MS/MS detection.
33301522	2	62	gly	N-glycopeptides	504:518	arg1	mixture					543:549	a tryptic peptide mixture	525:549	a tryptic peptide mixture using hydrophilic substances before LC-MS/MS detection	525:604	The sample pre-processing method used for the analysis of intact N-glycopeptides usually depends on the enrichment of low abundance N-glycopeptides from a tryptic peptide mixture using hydrophilic substances before LC-MS/MS detection.
33301522	2	62	gly	N-glycopeptides	504:518	arg2	N-glycopeptides					504:518	low abundance N-glycopeptides	490:518	low abundance N-glycopeptides from a tryptic peptide mixture using hydrophilic substances before LC-MS/MS detection	490:604	The sample pre-processing method used for the analysis of intact N-glycopeptides usually depends on the enrichment of low abundance N-glycopeptides from a tryptic peptide mixture using hydrophilic substances before LC-MS/MS detection.
33301522	8	63	theme	cells	1773:1777	arg1	N-glycoproteome					1740:1754	the N-glycoproteome	1736:1754	the N-glycoproteome of breast cancer cells cultured in different growth conditions	1736:1817	This study also provides an interesting comparison of the N-glycoproteome of breast cancer cells cultured in different growth conditions, indicating the important role of N-glycosylated proteins in cancer cell growth and the choice of the cell culture model for studies in tumor biology and drug evaluation.
33301522	6	64	theme	three-dimensional	1439:1455	arg1	cells					1485:1489	two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells	1414:1489	two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells	1414:1489	Furthermore, the optimized workflow was applied to the analysis of intact N-glycopeptides in two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells in vitro and xenografted tumors in mice.
33301522	6	65	dep	two-dimensional	1414:1428	arg1	2D					1431:1432	2D	1431:1432	2D	1431:1432	Furthermore, the optimized workflow was applied to the analysis of intact N-glycopeptides in two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells in vitro and xenografted tumors in mice.
33301522	7	66	theme	different	1608:1616	arg1	microenvironments					1618:1634	different microenvironments	1608:1634	different microenvironments	1608:1634	These results indicated that the same breast cancer cells, when cultured in different microenvironments, can show different N-glycosylation patterns.
33301522	4	67	theme	different	976:984	arg1	materials					997:1005	different enrichment materials	976:1005	different enrichment materials	976:1005	Here, we optimized the sample preparation workflow prior to LC-MS/MS analysis by systematically comparing different analytical methods, including the use of different sources of trypsin, combinations of different proteases, and different enrichment materials.
33301522	8	68	theme	N-glycosylated	1853:1866	arg1	proteins					1868:1875	N-glycosylated proteins	1853:1875	N-glycosylated proteins	1853:1875	This study also provides an interesting comparison of the N-glycoproteome of breast cancer cells cultured in different growth conditions, indicating the important role of N-glycosylated proteins in cancer cell growth and the choice of the cell culture model for studies in tumor biology and drug evaluation.
33301522	4	69	theme	materials	997:1005	arg1	use					898:900	the use	894:900	the use of different sources of trypsin, combinations of different proteases, and different enrichment materials	894:1005	Here, we optimized the sample preparation workflow prior to LC-MS/MS analysis by systematically comparing different analytical methods, including the use of different sources of trypsin, combinations of different proteases, and different enrichment materials.
33301522	5	70	gly	N-glycopeptides	1257:1271	arg2	N-glycopeptides					1257:1271	identified intact N-glycopeptides	1239:1271	identified intact N-glycopeptides	1239:1271	Finally, we found that the combination of Trypsin (B)/Lys-C digestion and zwitterionic HILIC (Zic-HILIC) enrichment significantly improved the mass spectrometric characterization of intact N-glycopeptides, increasing the number of identified intact N-glycopeptides and offering better analytical reproducibility.
33301522	8	71	theme	growth	1801:1806	arg1	conditions					1808:1817	different growth conditions	1791:1817	different growth conditions	1791:1817	This study also provides an interesting comparison of the N-glycoproteome of breast cancer cells cultured in different growth conditions, indicating the important role of N-glycosylated proteins in cancer cell growth and the choice of the cell culture model for studies in tumor biology and drug evaluation.
33301522	4	72	theme	different	951:959	arg1	proteases					961:969	different proteases	951:969	different proteases	951:969	Here, we optimized the sample preparation workflow prior to LC-MS/MS analysis by systematically comparing different analytical methods, including the use of different sources of trypsin, combinations of different proteases, and different enrichment materials.
33301522	7	73	theme	breast	1570:1575	arg1	cells					1584:1588	the same breast cancer cells	1561:1588	the same breast cancer cells	1561:1588	These results indicated that the same breast cancer cells, when cultured in different microenvironments, can show different N-glycosylation patterns.
33301522	2	74	theme	sample	376:381	arg1	method					398:403	The sample pre-processing method	372:403	The sample pre-processing method used for the analysis of intact N-glycopeptides	372:451	The sample pre-processing method used for the analysis of intact N-glycopeptides usually depends on the enrichment of low abundance N-glycopeptides from a tryptic peptide mixture using hydrophilic substances before LC-MS/MS detection.
33301522	8	75	theme	cell	1887:1890	arg1	growth					1892:1897	cancer cell growth	1880:1897	cancer cell growth	1880:1897	This study also provides an interesting comparison of the N-glycoproteome of breast cancer cells cultured in different growth conditions, indicating the important role of N-glycosylated proteins in cancer cell growth and the choice of the cell culture model for studies in tumor biology and drug evaluation.
33301522	7	76	theme	cancer	1577:1582	arg1	cells					1584:1588	the same breast cancer cells	1561:1588	the same breast cancer cells	1561:1588	These results indicated that the same breast cancer cells, when cultured in different microenvironments, can show different N-glycosylation patterns.
33301522	0	77	theme	tumors	159:164	arg1	proteome					63:70	the N-glycosylated proteome	44:70	the N-glycosylated proteome of two-dimensional- and three-dimensional-cultured breast cancer cells and xenografted tumors	44:164	In-depth characterization and comparison of the N-glycosylated proteome of two-dimensional- and three-dimensional-cultured breast cancer cells and xenografted tumors.
33301522	3	78	theme	intact	641:646	arg1	N-glycopeptides					648:662	identified intact N-glycopeptides	630:662	identified intact N-glycopeptides	630:662	However, the number of identified intact N-glycopeptides remains inadequate to achieve an in-depth profile of the N-glycosylation landscape.
33301522	1	79	theme	comprehensive	231:243	arg1	characteristics					245:259	the comprehensive characteristics	227:259	the comprehensive characteristics	227:259	Native intact N-glycopeptide analysis can provide access to the comprehensive characteristics of N-glycan occupancy, including N-glycosites, N-glycan compositions, and N-glycoproteins for complex samples.
33301522	1	79	theme	comprehensive	231:243	arg1	N-glycosites					294:305	N-glycosites	294:305	N-glycosites	294:305	Native intact N-glycopeptide analysis can provide access to the comprehensive characteristics of N-glycan occupancy, including N-glycosites, N-glycan compositions, and N-glycoproteins for complex samples.
33301522	1	79	theme	comprehensive	231:243	arg1	compositions					317:328	N-glycan compositions	308:328	N-glycan compositions	308:328	Native intact N-glycopeptide analysis can provide access to the comprehensive characteristics of N-glycan occupancy, including N-glycosites, N-glycan compositions, and N-glycoproteins for complex samples.
33301522	1	79	theme	comprehensive	231:243	arg1	N-glycoproteins					335:349	N-glycoproteins	335:349	N-glycoproteins	335:349	Native intact N-glycopeptide analysis can provide access to the comprehensive characteristics of N-glycan occupancy, including N-glycosites, N-glycan compositions, and N-glycoproteins for complex samples.
33301522	6	80	theme	cancer	1478:1483	arg1	cells					1485:1489	two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells	1414:1489	two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells	1414:1489	Furthermore, the optimized workflow was applied to the analysis of intact N-glycopeptides in two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells in vitro and xenografted tumors in mice.
33301522	5	81	theme	N-glycopeptides	1197:1211	arg1	characterization					1170:1185	the mass spectrometric characterization	1147:1185	the mass spectrometric characterization of intact N-glycopeptides	1147:1211	Finally, we found that the combination of Trypsin (B)/Lys-C digestion and zwitterionic HILIC (Zic-HILIC) enrichment significantly improved the mass spectrometric characterization of intact N-glycopeptides, increasing the number of identified intact N-glycopeptides and offering better analytical reproducibility.
33301522	6	82	theme	-cultured	1461:1469	arg1	cells					1485:1489	two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells	1414:1489	two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells	1414:1489	Furthermore, the optimized workflow was applied to the analysis of intact N-glycopeptides in two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells in vitro and xenografted tumors in mice.
33301522	4	83	theme	LC-MS/MS	808:815	arg1	analysis					817:824	LC-MS/MS analysis	808:824	LC-MS/MS analysis	808:824	Here, we optimized the sample preparation workflow prior to LC-MS/MS analysis by systematically comparing different analytical methods, including the use of different sources of trypsin, combinations of different proteases, and different enrichment materials.
33301522	6	84	from	analysis	1376:1383	arg1	mice					1526:1529	mice	1526:1529	mice	1526:1529	Furthermore, the optimized workflow was applied to the analysis of intact N-glycopeptides in two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells in vitro and xenografted tumors in mice.
33301522	6	84	from	analysis	1376:1383	arg1	cells					1485:1489	two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells	1414:1489	two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells	1414:1489	Furthermore, the optimized workflow was applied to the analysis of intact N-glycopeptides in two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells in vitro and xenografted tumors in mice.
33301522	5	85	theme	spectrometric	1156:1168	arg1	characterization					1170:1185	the mass spectrometric characterization	1147:1185	the mass spectrometric characterization of intact N-glycopeptides	1147:1211	Finally, we found that the combination of Trypsin (B)/Lys-C digestion and zwitterionic HILIC (Zic-HILIC) enrichment significantly improved the mass spectrometric characterization of intact N-glycopeptides, increasing the number of identified intact N-glycopeptides and offering better analytical reproducibility.
33301522	8	86	theme	culture	1926:1932	arg1	model					1934:1938	the cell culture model	1917:1938	the cell culture model	1917:1938	This study also provides an interesting comparison of the N-glycoproteome of breast cancer cells cultured in different growth conditions, indicating the important role of N-glycosylated proteins in cancer cell growth and the choice of the cell culture model for studies in tumor biology and drug evaluation.
33301522	6	87	gly	N-glycopeptides	1395:1409	arg2	N-glycopeptides					1395:1409	intact N-glycopeptides	1388:1409	intact N-glycopeptides in two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells	1388:1489	Furthermore, the optimized workflow was applied to the analysis of intact N-glycopeptides in two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells in vitro and xenografted tumors in mice.
33301522	0	88	theme	two-dimensional-	75:90	arg1	cells					137:141	two-dimensional- and three-dimensional-cultured breast cancer cells	75:141	two-dimensional- and three-dimensional-cultured breast cancer cells	75:141	In-depth characterization and comparison of the N-glycosylated proteome of two-dimensional- and three-dimensional-cultured breast cancer cells and xenografted tumors.
33301522	1	89	theme	intact	174:179	arg1	analysis					196:203	Native intact N-glycopeptide analysis	167:203	Native intact N-glycopeptide analysis	167:203	Native intact N-glycopeptide analysis can provide access to the comprehensive characteristics of N-glycan occupancy, including N-glycosites, N-glycan compositions, and N-glycoproteins for complex samples.
33301522	6	90	theme	xenografted	1504:1514	arg1	tumors					1516:1521	xenografted tumors	1504:1521	xenografted tumors in mice	1504:1529	Furthermore, the optimized workflow was applied to the analysis of intact N-glycopeptides in two-dimensional (2D) and three-dimensional (3D)-cultured breast cancer cells in vitro and xenografted tumors in mice.
33301522	0	91	theme	three-dimensional-cultured	96:121	arg1	cells					137:141	two-dimensional- and three-dimensional-cultured breast cancer cells	75:141	two-dimensional- and three-dimensional-cultured breast cancer cells	75:141	In-depth characterization and comparison of the N-glycosylated proteome of two-dimensional- and three-dimensional-cultured breast cancer cells and xenografted tumors.
33301522	5	92	theme	N-glycopeptides	1257:1271	arg1	number					1229:1234	the number	1225:1234	the number of identified intact N-glycopeptides	1225:1271	Finally, we found that the combination of Trypsin (B)/Lys-C digestion and zwitterionic HILIC (Zic-HILIC) enrichment significantly improved the mass spectrometric characterization of intact N-glycopeptides, increasing the number of identified intact N-glycopeptides and offering better analytical reproducibility.
33301522	2	93	theme	hydrophilic	557:567	arg1	substances					569:578	hydrophilic substances	557:578	hydrophilic substances before LC-MS/MS detection	557:604	The sample pre-processing method used for the analysis of intact N-glycopeptides usually depends on the enrichment of low abundance N-glycopeptides from a tryptic peptide mixture using hydrophilic substances before LC-MS/MS detection.
33301522	0	94	theme	cancer	130:135	arg1	cells					137:141	two-dimensional- and three-dimensional-cultured breast cancer cells	75:141	two-dimensional- and three-dimensional-cultured breast cancer cells	75:141	In-depth characterization and comparison of the N-glycosylated proteome of two-dimensional- and three-dimensional-cultured breast cancer cells and xenografted tumors.
33301522	8	95	from	choice	1907:1912	arg1	growth					1892:1897	cancer cell growth	1880:1897	cancer cell growth	1880:1897	This study also provides an interesting comparison of the N-glycoproteome of breast cancer cells cultured in different growth conditions, indicating the important role of N-glycosylated proteins in cancer cell growth and the choice of the cell culture model for studies in tumor biology and drug evaluation.
33301522	5	96	theme	/Lys-C	1061:1066	arg1	digestion					1068:1076	Trypsin (B)/Lys-C digestion	1050:1076	Trypsin (B)/Lys-C digestion	1050:1076	Finally, we found that the combination of Trypsin (B)/Lys-C digestion and zwitterionic HILIC (Zic-HILIC) enrichment significantly improved the mass spectrometric characterization of intact N-glycopeptides, increasing the number of identified intact N-glycopeptides and offering better analytical reproducibility.
33301522	8	97	theme	drug	1973:1976	arg1	evaluation					1978:1987	drug evaluation	1973:1987	drug evaluation	1973:1987	This study also provides an interesting comparison of the N-glycoproteome of breast cancer cells cultured in different growth conditions, indicating the important role of N-glycosylated proteins in cancer cell growth and the choice of the cell culture model for studies in tumor biology and drug evaluation.
33301522	5	98	theme	identified	1239:1248	arg1	N-glycopeptides					1257:1271	identified intact N-glycopeptides	1239:1271	identified intact N-glycopeptides	1239:1271	Finally, we found that the combination of Trypsin (B)/Lys-C digestion and zwitterionic HILIC (Zic-HILIC) enrichment significantly improved the mass spectrometric characterization of intact N-glycopeptides, increasing the number of identified intact N-glycopeptides and offering better analytical reproducibility.
33301522	5	99	theme	Trypsin	1050:1056	arg1	digestion					1068:1076	Trypsin (B)/Lys-C digestion	1050:1076	Trypsin (B)/Lys-C digestion	1050:1076	Finally, we found that the combination of Trypsin (B)/Lys-C digestion and zwitterionic HILIC (Zic-HILIC) enrichment significantly improved the mass spectrometric characterization of intact N-glycopeptides, increasing the number of identified intact N-glycopeptides and offering better analytical reproducibility.
33301522	2	100	theme	N-glycopeptides	437:451	arg1	analysis					418:425	the analysis	414:425	the analysis of intact N-glycopeptides	414:451	The sample pre-processing method used for the analysis of intact N-glycopeptides usually depends on the enrichment of low abundance N-glycopeptides from a tryptic peptide mixture using hydrophilic substances before LC-MS/MS detection.
33301522	0	101	gly	N-glycosylated	48:61	arg1	proteome					63:70	the N-glycosylated proteome	44:70	the N-glycosylated proteome of two-dimensional- and three-dimensional-cultured breast cancer cells and xenografted tumors	44:164	In-depth characterization and comparison of the N-glycosylated proteome of two-dimensional- and three-dimensional-cultured breast cancer cells and xenografted tumors.
33301522	5	102	theme	B	1059:1059	arg1	digestion					1068:1076	Trypsin (B)/Lys-C digestion	1050:1076	Trypsin (B)/Lys-C digestion	1050:1076	Finally, we found that the combination of Trypsin (B)/Lys-C digestion and zwitterionic HILIC (Zic-HILIC) enrichment significantly improved the mass spectrometric characterization of intact N-glycopeptides, increasing the number of identified intact N-glycopeptides and offering better analytical reproducibility.
33301522	2	103	theme	abundance	494:502	arg1	N-glycopeptides					504:518	low abundance N-glycopeptides	490:518	low abundance N-glycopeptides from a tryptic peptide mixture using hydrophilic substances before LC-MS/MS detection	490:604	The sample pre-processing method used for the analysis of intact N-glycopeptides usually depends on the enrichment of low abundance N-glycopeptides from a tryptic peptide mixture using hydrophilic substances before LC-MS/MS detection.
33301522	4	104	theme	analytical	864:873	arg1	methods					875:881	different analytical methods	854:881	different analytical methods	854:881	Here, we optimized the sample preparation workflow prior to LC-MS/MS analysis by systematically comparing different analytical methods, including the use of different sources of trypsin, combinations of different proteases, and different enrichment materials.
33301522	4	104	theme	analytical	864:873	arg1	use					898:900	the use	894:900	the use of different sources of trypsin, combinations of different proteases, and different enrichment materials	894:1005	Here, we optimized the sample preparation workflow prior to LC-MS/MS analysis by systematically comparing different analytical methods, including the use of different sources of trypsin, combinations of different proteases, and different enrichment materials.
33301522	1	105	theme	complex	355:361	arg1	samples					363:369	complex samples	355:369	complex samples	355:369	Native intact N-glycopeptide analysis can provide access to the comprehensive characteristics of N-glycan occupancy, including N-glycosites, N-glycan compositions, and N-glycoproteins for complex samples.
33301522	5	106	theme	enrichment	1113:1122	arg1	combination					1035:1045	the combination	1031:1045	the combination of Trypsin (B)/Lys-C digestion and zwitterionic HILIC (Zic-HILIC) enrichment	1031:1122	Finally, we found that the combination of Trypsin (B)/Lys-C digestion and zwitterionic HILIC (Zic-HILIC) enrichment significantly improved the mass spectrometric characterization of intact N-glycopeptides, increasing the number of identified intact N-glycopeptides and offering better analytical reproducibility.
33301522	3	107	theme	landscape	737:745	arg1	profile					706:712	an in-depth profile	694:712	an in-depth profile of the N-glycosylation landscape	694:745	However, the number of identified intact N-glycopeptides remains inadequate to achieve an in-depth profile of the N-glycosylation landscape.
33301522	4	108	theme	trypsin	926:932	arg1	sources					915:921	different sources	905:921	different sources of trypsin	905:932	Here, we optimized the sample preparation workflow prior to LC-MS/MS analysis by systematically comparing different analytical methods, including the use of different sources of trypsin, combinations of different proteases, and different enrichment materials.
33301522	4	108	theme	trypsin	926:932	arg1	materials					997:1005	different enrichment materials	976:1005	different enrichment materials	976:1005	Here, we optimized the sample preparation workflow prior to LC-MS/MS analysis by systematically comparing different analytical methods, including the use of different sources of trypsin, combinations of different proteases, and different enrichment materials.
33301522	4	108	theme	trypsin	926:932	arg1	combinations					935:946	combinations	935:946	combinations of different proteases	935:969	Here, we optimized the sample preparation workflow prior to LC-MS/MS analysis by systematically comparing different analytical methods, including the use of different sources of trypsin, combinations of different proteases, and different enrichment materials.
33301522	0	109	theme	proteome	63:70	arg1	comparison					30:39	comparison	30:39	comparison	30:39	In-depth characterization and comparison of the N-glycosylated proteome of two-dimensional- and three-dimensional-cultured breast cancer cells and xenografted tumors.
33301522	0	109	theme	proteome	63:70	arg1	characterization					9:24	In-depth characterization	0:24	In-depth characterization	0:24	In-depth characterization and comparison of the N-glycosylated proteome of two-dimensional- and three-dimensional-cultured breast cancer cells and xenografted tumors.
33301522	5	110	theme	analytical	1293:1302	arg1	reproducibility					1304:1318	better analytical reproducibility	1286:1318	better analytical reproducibility	1286:1318	Finally, we found that the combination of Trypsin (B)/Lys-C digestion and zwitterionic HILIC (Zic-HILIC) enrichment significantly improved the mass spectrometric characterization of intact N-glycopeptides, increasing the number of identified intact N-glycopeptides and offering better analytical reproducibility.
33301522	2	111	theme	tryptic	527:533	arg1	mixture					543:549	a tryptic peptide mixture	525:549	a tryptic peptide mixture using hydrophilic substances before LC-MS/MS detection	525:604	The sample pre-processing method used for the analysis of intact N-glycopeptides usually depends on the enrichment of low abundance N-glycopeptides from a tryptic peptide mixture using hydrophilic substances before LC-MS/MS detection.
33301522	4	112	theme	combinations	935:946	arg1	use					898:900	the use	894:900	the use of different sources of trypsin, combinations of different proteases, and different enrichment materials	894:1005	Here, we optimized the sample preparation workflow prior to LC-MS/MS analysis by systematically comparing different analytical methods, including the use of different sources of trypsin, combinations of different proteases, and different enrichment materials.
33301522	5	113	theme	HILIC	1095:1099	arg1	enrichment					1113:1122	zwitterionic HILIC (Zic-HILIC) enrichment	1082:1122	zwitterionic HILIC (Zic-HILIC) enrichment	1082:1122	Finally, we found that the combination of Trypsin (B)/Lys-C digestion and zwitterionic HILIC (Zic-HILIC) enrichment significantly improved the mass spectrometric characterization of intact N-glycopeptides, increasing the number of identified intact N-glycopeptides and offering better analytical reproducibility.
33301522	8	114	theme	cancer	1766:1771	arg1	cells					1773:1777	breast cancer cells	1759:1777	breast cancer cells cultured in different growth conditions	1759:1817	This study also provides an interesting comparison of the N-glycoproteome of breast cancer cells cultured in different growth conditions, indicating the important role of N-glycosylated proteins in cancer cell growth and the choice of the cell culture model for studies in tumor biology and drug evaluation.
33301522	8	115	theme	cell	1921:1924	arg1	model					1934:1938	the cell culture model	1917:1938	the cell culture model	1917:1938	This study also provides an interesting comparison of the N-glycoproteome of breast cancer cells cultured in different growth conditions, indicating the important role of N-glycosylated proteins in cancer cell growth and the choice of the cell culture model for studies in tumor biology and drug evaluation.
33301522	2	116	theme	pre-processing	383:396	arg1	method					398:403	The sample pre-processing method	372:403	The sample pre-processing method used for the analysis of intact N-glycopeptides	372:451	The sample pre-processing method used for the analysis of intact N-glycopeptides usually depends on the enrichment of low abundance N-glycopeptides from a tryptic peptide mixture using hydrophilic substances before LC-MS/MS detection.
33301522	8	117	theme	important	1835:1843	arg1	role					1845:1848	the important role	1831:1848	the important role of N-glycosylated proteins in cancer cell growth	1831:1897	This study also provides an interesting comparison of the N-glycoproteome of breast cancer cells cultured in different growth conditions, indicating the important role of N-glycosylated proteins in cancer cell growth and the choice of the cell culture model for studies in tumor biology and drug evaluation.
33301522	7	118	theme	different	1646:1654	arg1	patterns					1672:1679	different N-glycosylation patterns	1646:1679	different N-glycosylation patterns	1646:1679	These results indicated that the same breast cancer cells, when cultured in different microenvironments, can show different N-glycosylation patterns.
33301522	5	119	theme	Zic-HILIC	1102:1110	arg1	enrichment					1113:1122	zwitterionic HILIC (Zic-HILIC) enrichment	1082:1122	zwitterionic HILIC (Zic-HILIC) enrichment	1082:1122	Finally, we found that the combination of Trypsin (B)/Lys-C digestion and zwitterionic HILIC (Zic-HILIC) enrichment significantly improved the mass spectrometric characterization of intact N-glycopeptides, increasing the number of identified intact N-glycopeptides and offering better analytical reproducibility.
33301522	4	120	theme	sources	915:921	arg1	use					898:900	the use	894:900	the use of different sources of trypsin, combinations of different proteases, and different enrichment materials	894:1005	Here, we optimized the sample preparation workflow prior to LC-MS/MS analysis by systematically comparing different analytical methods, including the use of different sources of trypsin, combinations of different proteases, and different enrichment materials.
32678209	5	0	theme	Lactobacillus	760:772	arg1	levels					780:785	The Lactobacillus genus levels	756:785	The Lactobacillus genus levels	756:785	The Lactobacillus genus levels were increased by 3FN fermentation and B. breve by GNB and 3FN.
32678209	6	1	theme	group	906:910	arg1	reduction					875:883	a significant reduction	861:883	a significant reduction of Blautia coccoides group with LNB and 3FN	861:927	There was a significant reduction of Blautia coccoides group with LNB and 3FN.
32678209	2	2	theme	milk	363:366	arg1	oligosaccharides					368:383	single milk oligosaccharides	356:383	single milk oligosaccharides	356:383	The great diversity of the HMOs structures does not allow the attribution of specific prebiotic characteristics to single milk oligosaccharides.
32678209	10	3	from	application	1508:1518	arg1	foods					1530:1534	infant foods	1523:1534	infant foods	1523:1534	The results presented here indicated that each, LNB, GNB and 3FN disaccharide, might have a specific beneficial effect in the infant gut microbiota and they are potential prebiotics for application in infant foods.
32678209	5	4	theme	genus	774:778	arg1	levels					780:785	The Lactobacillus genus levels	756:785	The Lactobacillus genus levels	756:785	The Lactobacillus genus levels were increased by 3FN fermentation and B. breve by GNB and 3FN.
32678209	6	5	theme	coccoides	896:904	arg1	group					906:910	Blautia coccoides group	888:910	Blautia coccoides group	888:910	There was a significant reduction of Blautia coccoides group with LNB and 3FN.
32678209	10	6	theme	gut	1455:1457	arg1	microbiota					1459:1468	the infant gut microbiota	1444:1468	the infant gut microbiota	1444:1468	The results presented here indicated that each, LNB, GNB and 3FN disaccharide, might have a specific beneficial effect in the infant gut microbiota and they are potential prebiotics for application in infant foods.
32678209	2	7	theme	single	356:361	arg1	oligosaccharides					368:383	single milk oligosaccharides	356:383	single milk oligosaccharides	356:383	The great diversity of the HMOs structures does not allow the attribution of specific prebiotic characteristics to single milk oligosaccharides.
32678209	10	8	contain	have	1407:1410	arg1	disaccharide					1387:1398	3FN disaccharide	1383:1398	3FN disaccharide	1383:1398	The results presented here indicated that each, LNB, GNB and 3FN disaccharide, might have a specific beneficial effect in the infant gut microbiota and they are potential prebiotics for application in infant foods.
32678209	10	8	contain	have	1407:1410	arg1	LNB					1370:1372	LNB	1370:1372	LNB	1370:1372	The results presented here indicated that each, LNB, GNB and 3FN disaccharide, might have a specific beneficial effect in the infant gut microbiota and they are potential prebiotics for application in infant foods.
32678209	10	8	contain	have	1407:1410	arg1	each					1364:1367	each	1364:1367	each	1364:1367	The results presented here indicated that each, LNB, GNB and 3FN disaccharide, might have a specific beneficial effect in the infant gut microbiota and they are potential prebiotics for application in infant foods.
32678209	10	8	contain	have	1407:1410	arg2	effect					1434:1439	a specific beneficial effect	1412:1439	a specific beneficial effect	1412:1439	The results presented here indicated that each, LNB, GNB and 3FN disaccharide, might have a specific beneficial effect in the infant gut microbiota and they are potential prebiotics for application in infant foods.
32678209	10	8	contain	have	1407:1410	arg1	GNB					1375:1377	GNB	1375:1377	GNB	1375:1377	The results presented here indicated that each, LNB, GNB and 3FN disaccharide, might have a specific beneficial effect in the infant gut microbiota and they are potential prebiotics for application in infant foods.
32678209	6	9	theme	Blautia	888:894	arg1	group					906:910	Blautia coccoides group	888:910	Blautia coccoides group	888:910	There was a significant reduction of Blautia coccoides group with LNB and 3FN.
32678209	4	10	theme	Bifidobacterium	731:745	arg1	bifidum					747:753	Bifidobacterium bifidum	731:753	Bifidobacterium bifidum	731:753	LNB significantly increased the total levels of bifidobacteria and the species Bifidobacterium breve and Bifidobacterium bifidum.
32678209	4	10	theme	Bifidobacterium	731:745	arg1	species					697:703	the species Bifidobacterium breve and Bifidobacterium bifidum	693:753	the species Bifidobacterium breve and Bifidobacterium bifidum	693:753	LNB significantly increased the total levels of bifidobacteria and the species Bifidobacterium breve and Bifidobacterium bifidum.
32678209	7	11	theme	members	1009:1015	arg1	levels					973:978	the levels	969:978	the levels of Enterobacteriaceae family members	969:1015	In addition, 6FN significantly reduced the levels of Enterobacteriaceae family members.
32678209	10	12	theme	3FN	1383:1385	arg1	disaccharide					1387:1398	3FN disaccharide	1383:1398	3FN disaccharide	1383:1398	The results presented here indicated that each, LNB, GNB and 3FN disaccharide, might have a specific beneficial effect in the infant gut microbiota and they are potential prebiotics for application in infant foods.
32678209	10	12	theme	3FN	1383:1385	arg1	each					1364:1367	each	1364:1367	each	1364:1367	The results presented here indicated that each, LNB, GNB and 3FN disaccharide, might have a specific beneficial effect in the infant gut microbiota and they are potential prebiotics for application in infant foods.
32678209	4	13	theme	bifidobacteria	674:687	arg1	levels					664:669	the total levels	654:669	the total levels of bifidobacteria and the species Bifidobacterium breve and Bifidobacterium bifidum	654:753	LNB significantly increased the total levels of bifidobacteria and the species Bifidobacterium breve and Bifidobacterium bifidum.
32678209	4	14	theme	species	697:703	arg1	levels					664:669	the total levels	654:669	the total levels of bifidobacteria and the species Bifidobacterium breve and Bifidobacterium bifidum	654:753	LNB significantly increased the total levels of bifidobacteria and the species Bifidobacterium breve and Bifidobacterium bifidum.
32678209	8	15	theme	lactate	1057:1063	arg1	concentrations					1039:1052	Significantly higher concentrations	1018:1052	Significantly higher concentrations of lactate, formate and acetate	1018:1084	Significantly higher concentrations of lactate, formate and acetate were produced in cultures containing either LNB or GNB in comparison with control cultures.
32678209	2	16	theme	structures	273:282	arg1	diversity					251:259	The great diversity	241:259	The great diversity of the HMOs structures	241:282	The great diversity of the HMOs structures does not allow the attribution of specific prebiotic characteristics to single milk oligosaccharides.
32678209	1	17	theme	carbohydrates	164:176	arg1	oligosaccharides					102:117	Human milk oligosaccharides	91:117	Human milk oligosaccharides (HMOs)	91:124	Human milk oligosaccharides (HMOs) are a mixture of structurally diverse carbohydrates that contribute to shape a healthy gut microbiota composition.
32678209	1	17	theme	carbohydrates	164:176	arg1	mixture					132:138	a mixture	130:138	a mixture of structurally diverse carbohydrates that contribute to shape a healthy gut microbiota composition	130:238	Human milk oligosaccharides (HMOs) are a mixture of structurally diverse carbohydrates that contribute to shape a healthy gut microbiota composition.
32678209	8	18	theme	control	1160:1166	arg1	cultures					1168:1175	control cultures	1160:1175	control cultures	1160:1175	Significantly higher concentrations of lactate, formate and acetate were produced in cultures containing either LNB or GNB in comparison with control cultures.
32678209	0	19	from	impact	47:52	arg1	microbiota					79:88	cultured infant fecal microbiota	57:88	cultured infant fecal microbiota	57:88	Human milk and mucosa-associated disaccharides impact on cultured infant fecal microbiota.
32678209	10	20	theme	specific	1414:1421	arg1	effect					1434:1439	a specific beneficial effect	1412:1439	a specific beneficial effect	1412:1439	The results presented here indicated that each, LNB, GNB and 3FN disaccharide, might have a specific beneficial effect in the infant gut microbiota and they are potential prebiotics for application in infant foods.
32678209	2	21	theme	HMOs	268:271	arg1	structures					273:282	the HMOs structures	264:282	the HMOs structures	264:282	The great diversity of the HMOs structures does not allow the attribution of specific prebiotic characteristics to single milk oligosaccharides.
32678209	9	22	theme	fecal	1242:1246	arg1	microbiota					1248:1257	the fecal microbiota	1238:1257	the fecal microbiota	1238:1257	Additionally, after fermentation of the oligosaccharides by the fecal microbiota, several Bifidobacterium strains were isolated and identified.
32678209	5	23	theme	3FN	805:807	arg1	fermentation					809:820	3FN fermentation	805:820	3FN fermentation	805:820	The Lactobacillus genus levels were increased by 3FN fermentation and B. breve by GNB and 3FN.
32678209	8	24	theme	formate	1066:1072	arg1	concentrations					1039:1052	Significantly higher concentrations	1018:1052	Significantly higher concentrations of lactate, formate and acetate	1018:1084	Significantly higher concentrations of lactate, formate and acetate were produced in cultures containing either LNB or GNB in comparison with control cultures.
32678209	10	25	theme	beneficial	1423:1432	arg1	effect					1434:1439	a specific beneficial effect	1412:1439	a specific beneficial effect	1412:1439	The results presented here indicated that each, LNB, GNB and 3FN disaccharide, might have a specific beneficial effect in the infant gut microbiota and they are potential prebiotics for application in infant foods.
32678209	1	26	theme	Human	91:95	arg1	HMOs					120:123	HMOs	120:123	HMOs	120:123	Human milk oligosaccharides (HMOs) are a mixture of structurally diverse carbohydrates that contribute to shape a healthy gut microbiota composition.
32678209	1	26	theme	Human	91:95	arg1	oligosaccharides					102:117	Human milk oligosaccharides	91:117	Human milk oligosaccharides (HMOs)	91:124	Human milk oligosaccharides (HMOs) are a mixture of structurally diverse carbohydrates that contribute to shape a healthy gut microbiota composition.
32678209	1	26	theme	Human	91:95	arg1	mixture					132:138	a mixture	130:138	a mixture of structurally diverse carbohydrates that contribute to shape a healthy gut microbiota composition	130:238	Human milk oligosaccharides (HMOs) are a mixture of structurally diverse carbohydrates that contribute to shape a healthy gut microbiota composition.
32678209	0	27	theme	Human	0:4	arg1	milk					6:9	Human milk	0:9	Human milk	0:9	Human milk and mucosa-associated disaccharides impact on cultured infant fecal microbiota.
32678209	10	28	theme	infant	1448:1453	arg1	microbiota					1459:1468	the infant gut microbiota	1444:1468	the infant gut microbiota	1444:1468	The results presented here indicated that each, LNB, GNB and 3FN disaccharide, might have a specific beneficial effect in the infant gut microbiota and they are potential prebiotics for application in infant foods.
32678209	1	29	theme	milk	97:100	arg1	HMOs					120:123	HMOs	120:123	HMOs	120:123	Human milk oligosaccharides (HMOs) are a mixture of structurally diverse carbohydrates that contribute to shape a healthy gut microbiota composition.
32678209	1	29	theme	milk	97:100	arg1	oligosaccharides					102:117	Human milk oligosaccharides	91:117	Human milk oligosaccharides (HMOs)	91:124	Human milk oligosaccharides (HMOs) are a mixture of structurally diverse carbohydrates that contribute to shape a healthy gut microbiota composition.
32678209	1	29	theme	milk	97:100	arg1	mixture					132:138	a mixture	130:138	a mixture of structurally diverse carbohydrates that contribute to shape a healthy gut microbiota composition	130:238	Human milk oligosaccharides (HMOs) are a mixture of structurally diverse carbohydrates that contribute to shape a healthy gut microbiota composition.
32678209	0	30	theme	mucosa-associated	15:31	arg1	disaccharides					33:45	mucosa-associated disaccharides	15:45	mucosa-associated disaccharides	15:45	Human milk and mucosa-associated disaccharides impact on cultured infant fecal microbiota.
32678209	8	31	theme	acetate	1078:1084	arg1	concentrations					1039:1052	Significantly higher concentrations	1018:1052	Significantly higher concentrations of lactate, formate and acetate	1018:1084	Significantly higher concentrations of lactate, formate and acetate were produced in cultures containing either LNB or GNB in comparison with control cultures.
32678209	8	32	with	comparison	1144:1153	arg1	cultures					1168:1175	control cultures	1160:1175	control cultures	1160:1175	Significantly higher concentrations of lactate, formate and acetate were produced in cultures containing either LNB or GNB in comparison with control cultures.
32678209	3	33	gly	glycoprotein	569:580	arg1	glycoprotein					569:580	glycoprotein structures	569:591	glycoprotein structures	569:591	We analyze here the utilization of four disaccharides, lacto-N-biose (LNB), galacto-N-biose (GNB), fucosyl-α1,3-GlcNAc (3FN) and fucosyl-α1,6-GlcNAc (6FN), that form part of HMOs and glycoprotein structures, by the infant fecal microbiota.
32678209	4	34	dep	species	697:703	arg1	breve					721:725	Bifidobacterium breve	705:725	Bifidobacterium breve	705:725	LNB significantly increased the total levels of bifidobacteria and the species Bifidobacterium breve and Bifidobacterium bifidum.
32678209	4	34	dep	species	697:703	arg1	bifidum					747:753	Bifidobacterium bifidum	731:753	Bifidobacterium bifidum	731:753	LNB significantly increased the total levels of bifidobacteria and the species Bifidobacterium breve and Bifidobacterium bifidum.
32678209	4	34	dep	species	697:703	arg1	species					697:703	the species Bifidobacterium breve and Bifidobacterium bifidum	693:753	the species Bifidobacterium breve and Bifidobacterium bifidum	693:753	LNB significantly increased the total levels of bifidobacteria and the species Bifidobacterium breve and Bifidobacterium bifidum.
32678209	10	35	theme	potential	1483:1491	arg1	they					1474:1477	they	1474:1477	they	1474:1477	The results presented here indicated that each, LNB, GNB and 3FN disaccharide, might have a specific beneficial effect in the infant gut microbiota and they are potential prebiotics for application in infant foods.
32678209	10	35	theme	potential	1483:1491	arg1	prebiotics					1493:1502	potential prebiotics	1483:1502	potential prebiotics for application in infant foods	1483:1534	The results presented here indicated that each, LNB, GNB and 3FN disaccharide, might have a specific beneficial effect in the infant gut microbiota and they are potential prebiotics for application in infant foods.
32678209	1	36	theme	diverse	156:162	arg1	carbohydrates					164:176	structurally diverse carbohydrates	143:176	structurally diverse carbohydrates that contribute to shape a healthy gut microbiota composition	143:238	Human milk oligosaccharides (HMOs) are a mixture of structurally diverse carbohydrates that contribute to shape a healthy gut microbiota composition.
32678209	6	37	with	reduction	875:883	arg1	3FN					925:927	3FN	925:927	3FN	925:927	There was a significant reduction of Blautia coccoides group with LNB and 3FN.
32678209	6	37	with	reduction	875:883	arg1	LNB					917:919	LNB	917:919	LNB	917:919	There was a significant reduction of Blautia coccoides group with LNB and 3FN.
32678209	1	38	theme	healthy	205:211	arg1	composition					228:238	a healthy gut microbiota composition	203:238	a healthy gut microbiota composition	203:238	Human milk oligosaccharides (HMOs) are a mixture of structurally diverse carbohydrates that contribute to shape a healthy gut microbiota composition.
32678209	3	39	theme	disaccharides	426:438	arg1	utilization					406:416	the utilization	402:416	the utilization of four disaccharides, lacto-N-biose (LNB), galacto-N-biose (GNB), fucosyl-α1,3-GlcNAc (3FN) and fucosyl-α1,6-GlcNAc (6FN), that form part of HMOs and glycoprotein structures, by the infant fecal microbiota	402:623	We analyze here the utilization of four disaccharides, lacto-N-biose (LNB), galacto-N-biose (GNB), fucosyl-α1,3-GlcNAc (3FN) and fucosyl-α1,6-GlcNAc (6FN), that form part of HMOs and glycoprotein structures, by the infant fecal microbiota.
32678209	3	40	theme	infant	601:606	arg1	microbiota					614:623	the infant fecal microbiota	597:623	the infant fecal microbiota	597:623	We analyze here the utilization of four disaccharides, lacto-N-biose (LNB), galacto-N-biose (GNB), fucosyl-α1,3-GlcNAc (3FN) and fucosyl-α1,6-GlcNAc (6FN), that form part of HMOs and glycoprotein structures, by the infant fecal microbiota.
32678209	3	41	theme	glycoprotein	569:580	arg1	structures					582:591	glycoprotein structures	569:591	glycoprotein structures	569:591	We analyze here the utilization of four disaccharides, lacto-N-biose (LNB), galacto-N-biose (GNB), fucosyl-α1,3-GlcNAc (3FN) and fucosyl-α1,6-GlcNAc (6FN), that form part of HMOs and glycoprotein structures, by the infant fecal microbiota.
32678209	7	42	theme	family	1002:1007	arg1	members					1009:1015	Enterobacteriaceae family members	983:1015	Enterobacteriaceae family members	983:1015	In addition, 6FN significantly reduced the levels of Enterobacteriaceae family members.
32678209	1	43	theme	gut	213:215	arg1	composition					228:238	a healthy gut microbiota composition	203:238	a healthy gut microbiota composition	203:238	Human milk oligosaccharides (HMOs) are a mixture of structurally diverse carbohydrates that contribute to shape a healthy gut microbiota composition.
32678209	0	44	theme	cultured	57:64	arg1	microbiota					79:88	cultured infant fecal microbiota	57:88	cultured infant fecal microbiota	57:88	Human milk and mucosa-associated disaccharides impact on cultured infant fecal microbiota.
32678209	10	45	theme	infant	1523:1528	arg1	foods					1530:1534	infant foods	1523:1534	infant foods	1523:1534	The results presented here indicated that each, LNB, GNB and 3FN disaccharide, might have a specific beneficial effect in the infant gut microbiota and they are potential prebiotics for application in infant foods.
32678209	3	46	theme	fecal	608:612	arg1	microbiota					614:623	the infant fecal microbiota	597:623	the infant fecal microbiota	597:623	We analyze here the utilization of four disaccharides, lacto-N-biose (LNB), galacto-N-biose (GNB), fucosyl-α1,3-GlcNAc (3FN) and fucosyl-α1,6-GlcNAc (6FN), that form part of HMOs and glycoprotein structures, by the infant fecal microbiota.
32678209	6	47	theme	significant	863:873	arg1	reduction					875:883	a significant reduction	861:883	a significant reduction of Blautia coccoides group with LNB and 3FN	861:927	There was a significant reduction of Blautia coccoides group with LNB and 3FN.
32678209	8	48	contain	containing	1112:1121	arg1	cultures					1103:1110	cultures	1103:1110	cultures containing either LNB or GNB in comparison with control cultures	1103:1175	Significantly higher concentrations of lactate, formate and acetate were produced in cultures containing either LNB or GNB in comparison with control cultures.
32678209	8	48	contain	containing	1112:1121	arg2	GNB					1137:1139	GNB	1137:1139	GNB	1137:1139	Significantly higher concentrations of lactate, formate and acetate were produced in cultures containing either LNB or GNB in comparison with control cultures.
32678209	8	48	contain	containing	1112:1121	arg2	LNB					1130:1132	LNB	1130:1132	LNB	1130:1132	Significantly higher concentrations of lactate, formate and acetate were produced in cultures containing either LNB or GNB in comparison with control cultures.
32678209	8	49	theme	higher	1032:1037	arg1	concentrations					1039:1052	Significantly higher concentrations	1018:1052	Significantly higher concentrations of lactate, formate and acetate	1018:1084	Significantly higher concentrations of lactate, formate and acetate were produced in cultures containing either LNB or GNB in comparison with control cultures.
32678209	3	50	theme	HMOs	560:563	arg1	part					552:555	part	552:555	part of HMOs and glycoprotein structures	552:591	We analyze here the utilization of four disaccharides, lacto-N-biose (LNB), galacto-N-biose (GNB), fucosyl-α1,3-GlcNAc (3FN) and fucosyl-α1,6-GlcNAc (6FN), that form part of HMOs and glycoprotein structures, by the infant fecal microbiota.
32678209	2	51	theme	characteristics	337:351	arg1	attribution					303:313	the attribution	299:313	the attribution of specific prebiotic characteristics to single milk oligosaccharides	299:383	The great diversity of the HMOs structures does not allow the attribution of specific prebiotic characteristics to single milk oligosaccharides.
32678209	9	52	theme	oligosaccharides	1218:1233	arg1	fermentation					1198:1209	fermentation	1198:1209	fermentation of the oligosaccharides by the fecal microbiota	1198:1257	Additionally, after fermentation of the oligosaccharides by the fecal microbiota, several Bifidobacterium strains were isolated and identified.
32678209	9	53	theme	several	1260:1266	arg1	strains					1284:1290	several Bifidobacterium strains	1260:1290	several Bifidobacterium strains	1260:1290	Additionally, after fermentation of the oligosaccharides by the fecal microbiota, several Bifidobacterium strains were isolated and identified.
32678209	7	54	theme	Enterobacteriaceae	983:1000	arg1	members					1009:1015	Enterobacteriaceae family members	983:1015	Enterobacteriaceae family members	983:1015	In addition, 6FN significantly reduced the levels of Enterobacteriaceae family members.
32678209	2	55	theme	prebiotic	327:335	arg1	characteristics					337:351	specific prebiotic characteristics	318:351	specific prebiotic characteristics	318:351	The great diversity of the HMOs structures does not allow the attribution of specific prebiotic characteristics to single milk oligosaccharides.
32678209	1	56	theme	microbiota	217:226	arg1	composition					228:238	a healthy gut microbiota composition	203:238	a healthy gut microbiota composition	203:238	Human milk oligosaccharides (HMOs) are a mixture of structurally diverse carbohydrates that contribute to shape a healthy gut microbiota composition.
32678209	9	57	theme	Bifidobacterium	1268:1282	arg1	strains					1284:1290	several Bifidobacterium strains	1260:1290	several Bifidobacterium strains	1260:1290	Additionally, after fermentation of the oligosaccharides by the fecal microbiota, several Bifidobacterium strains were isolated and identified.
32678209	5	58	theme	B.	826:827	arg1	breve					829:833	B. breve	826:833	B. breve	826:833	The Lactobacillus genus levels were increased by 3FN fermentation and B. breve by GNB and 3FN.
32678209	2	59	theme	specific	318:325	arg1	characteristics					337:351	specific prebiotic characteristics	318:351	specific prebiotic characteristics	318:351	The great diversity of the HMOs structures does not allow the attribution of specific prebiotic characteristics to single milk oligosaccharides.
32678209	4	60	theme	Bifidobacterium	705:719	arg1	breve					721:725	Bifidobacterium breve	705:725	Bifidobacterium breve	705:725	LNB significantly increased the total levels of bifidobacteria and the species Bifidobacterium breve and Bifidobacterium bifidum.
32678209	4	60	theme	Bifidobacterium	705:719	arg1	species					697:703	the species Bifidobacterium breve and Bifidobacterium bifidum	693:753	the species Bifidobacterium breve and Bifidobacterium bifidum	693:753	LNB significantly increased the total levels of bifidobacteria and the species Bifidobacterium breve and Bifidobacterium bifidum.
32678209	0	61	theme	fecal	73:77	arg1	microbiota					79:88	cultured infant fecal microbiota	57:88	cultured infant fecal microbiota	57:88	Human milk and mucosa-associated disaccharides impact on cultured infant fecal microbiota.
32678209	3	62	theme	structures	582:591	arg1	part					552:555	part	552:555	part of HMOs and glycoprotein structures	552:591	We analyze here the utilization of four disaccharides, lacto-N-biose (LNB), galacto-N-biose (GNB), fucosyl-α1,3-GlcNAc (3FN) and fucosyl-α1,6-GlcNAc (6FN), that form part of HMOs and glycoprotein structures, by the infant fecal microbiota.
32678209	0	63	theme	infant	66:71	arg1	microbiota					79:88	cultured infant fecal microbiota	57:88	cultured infant fecal microbiota	57:88	Human milk and mucosa-associated disaccharides impact on cultured infant fecal microbiota.
32678209	2	64	theme	great	245:249	arg1	diversity					251:259	The great diversity	241:259	The great diversity of the HMOs structures	241:282	The great diversity of the HMOs structures does not allow the attribution of specific prebiotic characteristics to single milk oligosaccharides.
32678209	4	65	theme	total	658:662	arg1	levels					664:669	the total levels	654:669	the total levels of bifidobacteria and the species Bifidobacterium breve and Bifidobacterium bifidum	654:753	LNB significantly increased the total levels of bifidobacteria and the species Bifidobacterium breve and Bifidobacterium bifidum.
32284627	0	0	theme	HUMAN	85:89	arg1	COLOSTRUM					91:99	HUMAN COLOSTRUM	85:99	HUMAN COLOSTRUM ACCORDING TO THE CHEMICAL COMPOSITION OF THE ANTIGEN	85:152	ANTIGENIC STIMULATION DURING PREGNANCY MODIFIES SPECIFIC IGA1 AND IGA2 SUBCLASSES IN HUMAN COLOSTRUM ACCORDING TO THE CHEMICAL COMPOSITION OF THE ANTIGEN.
32284627	5	1	from	levels	626:631	arg1	milk					645:648	maternal milk	636:648	maternal milk	636:648	Total IgA levels in maternal milk vary after antigen stimulation and have differential affinities in function of the chemical composition of the antigens.
32284627	1	2	theme	BACKGROUND	155:164	arg1	studies					174:180	BACKGROUND Several studies	155:180	BACKGROUND Several studies	155:180	BACKGROUND Several studies have evaluated the effect of infectious diseases and vaccine protocols during pregnancy on maternal milk immunoglobulin (Ig) levels, to understand the protection conferred by lactation on newborns.
32284627	8	3	from	RESULTS	1201:1207	arg1	agreement					1212:1220	agreement	1212:1220	agreement with the previous reports, we	1212:1250	RESULTS In agreement with the previous reports, we showed that IgA1 from colostrum mainly recognized protein antigens; in sharp contrast, IgA2 was mostly directed against polysaccharide antigens.
32284627	7	4	theme	tetanus	1076:1082	arg1	toxoid					1084:1089	tetanus toxoid	1076:1089	tetanus toxoid	1076:1089	METHODS We analyzed data from 113 women in Mexico City and compared the amount of IgA subclasses in colostrum against three antigens: two from vaccine protocols (tetanus toxoid and pneumococcal polysaccharides) and lipopolysaccharide, a ubiquitous antigen in the gastrointestinal tract.
32284627	7	5	dep	METHODS	914:920	arg1	two					1048:1050	two	1048:1050	two	1048:1050	METHODS We analyzed data from 113 women in Mexico City and compared the amount of IgA subclasses in colostrum against three antigens: two from vaccine protocols (tetanus toxoid and pneumococcal polysaccharides) and lipopolysaccharide, a ubiquitous antigen in the gastrointestinal tract.
32284627	7	5	dep	METHODS	914:920	arg1	analyzed					925:932	analyzed	925:932	analyzed data from 113 women in Mexico City	925:967	METHODS We analyzed data from 113 women in Mexico City and compared the amount of IgA subclasses in colostrum against three antigens: two from vaccine protocols (tetanus toxoid and pneumococcal polysaccharides) and lipopolysaccharide, a ubiquitous antigen in the gastrointestinal tract.
32284627	7	5	dep	METHODS	914:920	arg1	compared					973:980	compared	973:980	compared the amount of IgA subclasses in colostrum against three antigens	973:1045	METHODS We analyzed data from 113 women in Mexico City and compared the amount of IgA subclasses in colostrum against three antigens: two from vaccine protocols (tetanus toxoid and pneumococcal polysaccharides) and lipopolysaccharide, a ubiquitous antigen in the gastrointestinal tract.
32284627	1	6	theme	Several	166:172	arg1	studies					174:180	BACKGROUND Several studies	155:180	BACKGROUND Several studies	155:180	BACKGROUND Several studies have evaluated the effect of infectious diseases and vaccine protocols during pregnancy on maternal milk immunoglobulin (Ig) levels, to understand the protection conferred by lactation on newborns.
32284627	4	7	theme	anatomical	564:573	arg1	distribution					575:586	differential anatomical distribution	551:586	differential anatomical distribution among mucosal compartments	551:613	In humans, IgA has two subclasses with differential anatomical distribution among mucosal compartments.
32284627	5	8	theme	chemical	733:740	arg1	composition					742:752	the chemical composition	729:752	the chemical composition of the antigens	729:768	Total IgA levels in maternal milk vary after antigen stimulation and have differential affinities in function of the chemical composition of the antigens.
32284627	7	9	from	antigen	1162:1168	arg1	tract					1194:1198	the gastrointestinal tract	1173:1198	the gastrointestinal tract	1173:1198	METHODS We analyzed data from 113 women in Mexico City and compared the amount of IgA subclasses in colostrum against three antigens: two from vaccine protocols (tetanus toxoid and pneumococcal polysaccharides) and lipopolysaccharide, a ubiquitous antigen in the gastrointestinal tract.
32284627	5	10	theme	antigen	661:667	arg1	stimulation					669:679	antigen stimulation	661:679	antigen stimulation	661:679	Total IgA levels in maternal milk vary after antigen stimulation and have differential affinities in function of the chemical composition of the antigens.
32284627	8	11	theme	previous	1231:1238	arg1	we					1249:1250	we	1249:1250	we	1249:1250	RESULTS In agreement with the previous reports, we showed that IgA1 from colostrum mainly recognized protein antigens; in sharp contrast, IgA2 was mostly directed against polysaccharide antigens.
32284627	8	11	theme	previous	1231:1238	arg1	reports					1240:1246	the previous reports	1227:1246	the previous reports	1227:1246	RESULTS In agreement with the previous reports, we showed that IgA1 from colostrum mainly recognized protein antigens; in sharp contrast, IgA2 was mostly directed against polysaccharide antigens.
32284627	10	12	theme	antigen	1650:1656	arg1	composition					1631:1641	the chemical composition	1618:1641	the chemical composition of the antigen	1618:1656	CONCLUSIONS Antigen interaction during pregnancy increased the amount of specific IgA subclasses, depending on the chemical composition of the antigen.
32284627	1	13	theme	maternal	273:280	arg1	levels					307:312	maternal milk immunoglobulin (Ig) levels	273:312	maternal milk immunoglobulin (Ig) levels	273:312	BACKGROUND Several studies have evaluated the effect of infectious diseases and vaccine protocols during pregnancy on maternal milk immunoglobulin (Ig) levels, to understand the protection conferred by lactation on newborns.
32284627	5	14	theme	composition	742:752	arg1	function					717:724	function	717:724	function of the chemical composition of the antigens	717:768	Total IgA levels in maternal milk vary after antigen stimulation and have differential affinities in function of the chemical composition of the antigens.
32284627	5	15	theme	Total	616:620	arg1	levels					626:631	Total IgA levels	616:631	Total IgA levels in maternal milk	616:648	Total IgA levels in maternal milk vary after antigen stimulation and have differential affinities in function of the chemical composition of the antigens.
32284627	4	16	contain	has	527:529	arg1	IgA					523:525	IgA	523:525	IgA	523:525	In humans, IgA has two subclasses with differential anatomical distribution among mucosal compartments.
32284627	4	16	contain	has	527:529	arg2	subclasses					535:544	two subclasses	531:544	two subclasses	531:544	In humans, IgA has two subclasses with differential anatomical distribution among mucosal compartments.
32284627	1	17	theme	milk	282:285	arg1	Ig					303:304	Ig	303:304	Ig	303:304	BACKGROUND Several studies have evaluated the effect of infectious diseases and vaccine protocols during pregnancy on maternal milk immunoglobulin (Ig) levels, to understand the protection conferred by lactation on newborns.
32284627	1	17	theme	milk	282:285	arg1	immunoglobulin					287:300	milk immunoglobulin	282:300	maternal milk immunoglobulin (Ig) levels	273:312	BACKGROUND Several studies have evaluated the effect of infectious diseases and vaccine protocols during pregnancy on maternal milk immunoglobulin (Ig) levels, to understand the protection conferred by lactation on newborns.
32284627	3	18	theme	protection	465:474	arg1	mechanisms					476:485	protection mechanisms	465:485	protection mechanisms in the neonate's mucosa	465:509	IgA participates in protection mechanisms in the neonate's mucosa.
32284627	9	19	theme	previous	1437:1444	arg1	contacts					1446:1453	previous contacts	1437:1453	previous contacts	1437:1453	These levels increased in women who had previous contacts through vaccination or infections during pregnancy.
32284627	2	20	theme	primary	397:403	arg1	Colostrum					380:388	Colostrum	380:388	Colostrum	380:388	Colostrum is the primary source of maternal IgA for the newborn.
32284627	2	20	theme	primary	397:403	arg1	source					405:410	the primary source	393:410	the primary source of maternal IgA for the newborn	393:442	Colostrum is the primary source of maternal IgA for the newborn.
32284627	6	21	from	colostrum	903:911	arg1	concentrations					842:855	the concentrations	838:855	the concentrations of specific IgA1 and IgA2 subclasses in human colostrum	838:911	We studied the effect of antigenic stimulation during pregnancy on the concentrations of specific IgA1 and IgA2 subclasses in human colostrum.
32284627	6	22	theme	human	897:901	arg1	colostrum					903:911	human colostrum	897:911	human colostrum	897:911	We studied the effect of antigenic stimulation during pregnancy on the concentrations of specific IgA1 and IgA2 subclasses in human colostrum.
32284627	6	23	from	concentrations	842:855	arg1	colostrum					903:911	human colostrum	897:911	human colostrum	897:911	We studied the effect of antigenic stimulation during pregnancy on the concentrations of specific IgA1 and IgA2 subclasses in human colostrum.
32284627	3	24	from	mechanisms	476:485	arg1	mucosa					504:509	the neonate's mucosa	490:509	the neonate's mucosa	490:509	IgA participates in protection mechanisms in the neonate's mucosa.
32284627	10	25	theme	subclasses	1593:1602	arg1	subclasses					1593:1602	specific IgA subclasses	1580:1602	specific IgA subclasses	1580:1602	CONCLUSIONS Antigen interaction during pregnancy increased the amount of specific IgA subclasses, depending on the chemical composition of the antigen.
32284627	10	25	theme	subclasses	1593:1602	arg1	amount					1570:1575	the amount	1566:1575	the amount of specific IgA subclasses	1566:1602	CONCLUSIONS Antigen interaction during pregnancy increased the amount of specific IgA subclasses, depending on the chemical composition of the antigen.
32284627	7	26	theme	gastrointestinal	1177:1192	arg1	tract					1194:1198	the gastrointestinal tract	1173:1198	the gastrointestinal tract	1173:1198	METHODS We analyzed data from 113 women in Mexico City and compared the amount of IgA subclasses in colostrum against three antigens: two from vaccine protocols (tetanus toxoid and pneumococcal polysaccharides) and lipopolysaccharide, a ubiquitous antigen in the gastrointestinal tract.
32284627	5	27	theme	maternal	636:643	arg1	milk					645:648	maternal milk	636:648	maternal milk	636:648	Total IgA levels in maternal milk vary after antigen stimulation and have differential affinities in function of the chemical composition of the antigens.
32284627	9	28	contain	had	1433:1435	arg2	contacts					1446:1453	previous contacts	1437:1453	previous contacts	1437:1453	These levels increased in women who had previous contacts through vaccination or infections during pregnancy.
32284627	9	28	contain	had	1433:1435	arg1	women					1423:1427	women	1423:1427	women who had previous contacts through vaccination or infections during pregnancy	1423:1504	These levels increased in women who had previous contacts through vaccination or infections during pregnancy.
32284627	8	29	theme	polysaccharide	1372:1385	arg1	antigens					1387:1394	polysaccharide antigens	1372:1394	polysaccharide antigens	1372:1394	RESULTS In agreement with the previous reports, we showed that IgA1 from colostrum mainly recognized protein antigens; in sharp contrast, IgA2 was mostly directed against polysaccharide antigens.
32284627	0	30	theme	ANTIGENIC	0:8	arg1	STIMULATION					10:20	ANTIGENIC STIMULATION	0:20	ANTIGENIC STIMULATION DURING PREGNANCY	0:37	ANTIGENIC STIMULATION DURING PREGNANCY MODIFIES SPECIFIC IGA1 AND IGA2 SUBCLASSES IN HUMAN COLOSTRUM ACCORDING TO THE CHEMICAL COMPOSITION OF THE ANTIGEN.
32284627	1	31	theme	immunoglobulin	287:300	arg1	levels					307:312	maternal milk immunoglobulin (Ig) levels	273:312	maternal milk immunoglobulin (Ig) levels	273:312	BACKGROUND Several studies have evaluated the effect of infectious diseases and vaccine protocols during pregnancy on maternal milk immunoglobulin (Ig) levels, to understand the protection conferred by lactation on newborns.
32284627	7	32	theme	ubiquitous	1151:1160	arg1	antigen					1162:1168	a ubiquitous antigen	1149:1168	a ubiquitous antigen in the gastrointestinal tract	1149:1198	METHODS We analyzed data from 113 women in Mexico City and compared the amount of IgA subclasses in colostrum against three antigens: two from vaccine protocols (tetanus toxoid and pneumococcal polysaccharides) and lipopolysaccharide, a ubiquitous antigen in the gastrointestinal tract.
32284627	7	32	theme	ubiquitous	1151:1160	arg1	lipopolysaccharide					1129:1146	lipopolysaccharide	1129:1146	lipopolysaccharide	1129:1146	METHODS We analyzed data from 113 women in Mexico City and compared the amount of IgA subclasses in colostrum against three antigens: two from vaccine protocols (tetanus toxoid and pneumococcal polysaccharides) and lipopolysaccharide, a ubiquitous antigen in the gastrointestinal tract.
32284627	6	33	theme	specific	860:867	arg1	subclasses					883:892	specific IgA1 and IgA2 subclasses	860:892	specific IgA1 and IgA2 subclasses in human colostrum	860:911	We studied the effect of antigenic stimulation during pregnancy on the concentrations of specific IgA1 and IgA2 subclasses in human colostrum.
32284627	8	34	from	colostrum	1274:1282	arg1	IgA1					1264:1267	IgA1	1264:1267	IgA1 from colostrum	1264:1282	RESULTS In agreement with the previous reports, we showed that IgA1 from colostrum mainly recognized protein antigens; in sharp contrast, IgA2 was mostly directed against polysaccharide antigens.
32284627	6	35	from	subclasses	883:892	arg1	colostrum					903:911	human colostrum	897:911	human colostrum	897:911	We studied the effect of antigenic stimulation during pregnancy on the concentrations of specific IgA1 and IgA2 subclasses in human colostrum.
32284627	10	36	theme	CONCLUSIONS	1507:1517	arg1	interaction					1527:1537	CONCLUSIONS Antigen interaction	1507:1537	CONCLUSIONS Antigen interaction during pregnancy	1507:1554	CONCLUSIONS Antigen interaction during pregnancy increased the amount of specific IgA subclasses, depending on the chemical composition of the antigen.
32284627	5	37	theme	IgA	622:624	arg1	levels					626:631	Total IgA levels	616:631	Total IgA levels in maternal milk	616:648	Total IgA levels in maternal milk vary after antigen stimulation and have differential affinities in function of the chemical composition of the antigens.
32284627	2	38	theme	IgA	424:426	arg1	Colostrum					380:388	Colostrum	380:388	Colostrum	380:388	Colostrum is the primary source of maternal IgA for the newborn.
32284627	2	38	theme	IgA	424:426	arg1	source					405:410	the primary source	393:410	the primary source of maternal IgA for the newborn	393:442	Colostrum is the primary source of maternal IgA for the newborn.
32284627	7	39	theme	IgA	996:998	arg1	subclasses					1000:1009	IgA subclasses	996:1009	IgA subclasses	996:1009	METHODS We analyzed data from 113 women in Mexico City and compared the amount of IgA subclasses in colostrum against three antigens: two from vaccine protocols (tetanus toxoid and pneumococcal polysaccharides) and lipopolysaccharide, a ubiquitous antigen in the gastrointestinal tract.
32284627	4	40	theme	differential	551:562	arg1	distribution					575:586	differential anatomical distribution	551:586	differential anatomical distribution among mucosal compartments	551:613	In humans, IgA has two subclasses with differential anatomical distribution among mucosal compartments.
32284627	0	41	theme	CHEMICAL	118:125	arg1	COMPOSITION					127:137	THE CHEMICAL COMPOSITION	114:137	THE CHEMICAL COMPOSITION OF THE ANTIGEN	114:152	ANTIGENIC STIMULATION DURING PREGNANCY MODIFIES SPECIFIC IGA1 AND IGA2 SUBCLASSES IN HUMAN COLOSTRUM ACCORDING TO THE CHEMICAL COMPOSITION OF THE ANTIGEN.
32284627	6	42	theme	subclasses	883:892	arg1	concentrations					842:855	the concentrations	838:855	the concentrations of specific IgA1 and IgA2 subclasses in human colostrum	838:911	We studied the effect of antigenic stimulation during pregnancy on the concentrations of specific IgA1 and IgA2 subclasses in human colostrum.
32284627	10	43	theme	IgA	1589:1591	arg1	subclasses					1593:1602	specific IgA subclasses	1580:1602	specific IgA subclasses	1580:1602	CONCLUSIONS Antigen interaction during pregnancy increased the amount of specific IgA subclasses, depending on the chemical composition of the antigen.
32284627	10	44	theme	specific	1580:1587	arg1	subclasses					1593:1602	specific IgA subclasses	1580:1602	specific IgA subclasses	1580:1602	CONCLUSIONS Antigen interaction during pregnancy increased the amount of specific IgA subclasses, depending on the chemical composition of the antigen.
32284627	2	45	theme	maternal	415:422	arg1	IgA					424:426	maternal IgA	415:426	maternal IgA	415:426	Colostrum is the primary source of maternal IgA for the newborn.
32284627	0	46	theme	SPECIFIC	48:55	arg1	SUBCLASSES					71:80	SPECIFIC IGA1 AND IGA2 SUBCLASSES	48:80	SPECIFIC IGA1 AND IGA2 SUBCLASSES	48:80	ANTIGENIC STIMULATION DURING PREGNANCY MODIFIES SPECIFIC IGA1 AND IGA2 SUBCLASSES IN HUMAN COLOSTRUM ACCORDING TO THE CHEMICAL COMPOSITION OF THE ANTIGEN.
32284627	10	47	theme	Antigen	1519:1525	arg1	interaction					1527:1537	CONCLUSIONS Antigen interaction	1507:1537	CONCLUSIONS Antigen interaction during pregnancy	1507:1554	CONCLUSIONS Antigen interaction during pregnancy increased the amount of specific IgA subclasses, depending on the chemical composition of the antigen.
32284627	6	48	theme	IgA2	878:881	arg1	subclasses					883:892	specific IgA1 and IgA2 subclasses	860:892	specific IgA1 and IgA2 subclasses in human colostrum	860:911	We studied the effect of antigenic stimulation during pregnancy on the concentrations of specific IgA1 and IgA2 subclasses in human colostrum.
32284627	1	49	theme	infectious	211:220	arg1	diseases					222:229	infectious diseases	211:229	infectious diseases	211:229	BACKGROUND Several studies have evaluated the effect of infectious diseases and vaccine protocols during pregnancy on maternal milk immunoglobulin (Ig) levels, to understand the protection conferred by lactation on newborns.
32284627	7	50	theme	vaccine	1057:1063	arg1	protocols					1065:1073	vaccine protocols	1057:1073	vaccine protocols (tetanus toxoid and pneumococcal polysaccharides)	1057:1123	METHODS We analyzed data from 113 women in Mexico City and compared the amount of IgA subclasses in colostrum against three antigens: two from vaccine protocols (tetanus toxoid and pneumococcal polysaccharides) and lipopolysaccharide, a ubiquitous antigen in the gastrointestinal tract.
32284627	8	51	with	agreement	1212:1220	arg1	we					1249:1250	we	1249:1250	we	1249:1250	RESULTS In agreement with the previous reports, we showed that IgA1 from colostrum mainly recognized protein antigens; in sharp contrast, IgA2 was mostly directed against polysaccharide antigens.
32284627	8	51	with	agreement	1212:1220	arg1	reports					1240:1246	the previous reports	1227:1246	the previous reports	1227:1246	RESULTS In agreement with the previous reports, we showed that IgA1 from colostrum mainly recognized protein antigens; in sharp contrast, IgA2 was mostly directed against polysaccharide antigens.
32284627	1	52	theme	diseases	222:229	arg1	effect					201:206	the effect	197:206	the effect of infectious diseases and vaccine protocols during pregnancy on maternal milk immunoglobulin (Ig) levels	197:312	BACKGROUND Several studies have evaluated the effect of infectious diseases and vaccine protocols during pregnancy on maternal milk immunoglobulin (Ig) levels, to understand the protection conferred by lactation on newborns.
32284627	6	53	theme	IgA1	869:872	arg1	subclasses					883:892	specific IgA1 and IgA2 subclasses	860:892	specific IgA1 and IgA2 subclasses in human colostrum	860:911	We studied the effect of antigenic stimulation during pregnancy on the concentrations of specific IgA1 and IgA2 subclasses in human colostrum.
32284627	7	54	theme	subclasses	1000:1009	arg1	amount					986:991	the amount	982:991	the amount of IgA subclasses	982:1009	METHODS We analyzed data from 113 women in Mexico City and compared the amount of IgA subclasses in colostrum against three antigens: two from vaccine protocols (tetanus toxoid and pneumococcal polysaccharides) and lipopolysaccharide, a ubiquitous antigen in the gastrointestinal tract.
32284627	7	54	theme	subclasses	1000:1009	arg1	subclasses					1000:1009	IgA subclasses	996:1009	IgA subclasses	996:1009	METHODS We analyzed data from 113 women in Mexico City and compared the amount of IgA subclasses in colostrum against three antigens: two from vaccine protocols (tetanus toxoid and pneumococcal polysaccharides) and lipopolysaccharide, a ubiquitous antigen in the gastrointestinal tract.
32284627	8	55	theme	protein	1302:1308	arg1	antigens					1310:1317	protein antigens	1302:1317	protein antigens	1302:1317	RESULTS In agreement with the previous reports, we showed that IgA1 from colostrum mainly recognized protein antigens; in sharp contrast, IgA2 was mostly directed against polysaccharide antigens.
32284627	0	56	theme	IGA1	57:60	arg1	SUBCLASSES					71:80	SPECIFIC IGA1 AND IGA2 SUBCLASSES	48:80	SPECIFIC IGA1 AND IGA2 SUBCLASSES	48:80	ANTIGENIC STIMULATION DURING PREGNANCY MODIFIES SPECIFIC IGA1 AND IGA2 SUBCLASSES IN HUMAN COLOSTRUM ACCORDING TO THE CHEMICAL COMPOSITION OF THE ANTIGEN.
32284627	5	57	contain	have	685:688	arg1	levels					626:631	Total IgA levels	616:631	Total IgA levels in maternal milk	616:648	Total IgA levels in maternal milk vary after antigen stimulation and have differential affinities in function of the chemical composition of the antigens.
32284627	5	57	contain	have	685:688	arg2	affinities					703:712	differential affinities	690:712	differential affinities	690:712	Total IgA levels in maternal milk vary after antigen stimulation and have differential affinities in function of the chemical composition of the antigens.
32284627	0	58	theme	ANTIGEN	146:152	arg1	COMPOSITION					127:137	THE CHEMICAL COMPOSITION	114:137	THE CHEMICAL COMPOSITION OF THE ANTIGEN	114:152	ANTIGENIC STIMULATION DURING PREGNANCY MODIFIES SPECIFIC IGA1 AND IGA2 SUBCLASSES IN HUMAN COLOSTRUM ACCORDING TO THE CHEMICAL COMPOSITION OF THE ANTIGEN.
32284627	4	59	theme	mucosal	594:600	arg1	compartments					602:613	mucosal compartments	594:613	mucosal compartments	594:613	In humans, IgA has two subclasses with differential anatomical distribution among mucosal compartments.
32284627	7	60	theme	pneumococcal	1095:1106	arg1	polysaccharides					1108:1122	pneumococcal polysaccharides	1095:1122	pneumococcal polysaccharides	1095:1122	METHODS We analyzed data from 113 women in Mexico City and compared the amount of IgA subclasses in colostrum against three antigens: two from vaccine protocols (tetanus toxoid and pneumococcal polysaccharides) and lipopolysaccharide, a ubiquitous antigen in the gastrointestinal tract.
32284627	1	61	from	effect	201:206	arg1	levels					307:312	maternal milk immunoglobulin (Ig) levels	273:312	maternal milk immunoglobulin (Ig) levels	273:312	BACKGROUND Several studies have evaluated the effect of infectious diseases and vaccine protocols during pregnancy on maternal milk immunoglobulin (Ig) levels, to understand the protection conferred by lactation on newborns.
32284627	5	62	theme	differential	690:701	arg1	affinities					703:712	differential affinities	690:712	differential affinities	690:712	Total IgA levels in maternal milk vary after antigen stimulation and have differential affinities in function of the chemical composition of the antigens.
32284627	6	63	theme	antigenic	796:804	arg1	stimulation					806:816	antigenic stimulation	796:816	antigenic stimulation during pregnancy	796:833	We studied the effect of antigenic stimulation during pregnancy on the concentrations of specific IgA1 and IgA2 subclasses in human colostrum.
32284627	7	64	from	women	948:952	arg1	City					964:967	City	964:967	City	964:967	METHODS We analyzed data from 113 women in Mexico City and compared the amount of IgA subclasses in colostrum against three antigens: two from vaccine protocols (tetanus toxoid and pneumococcal polysaccharides) and lipopolysaccharide, a ubiquitous antigen in the gastrointestinal tract.
32284627	7	64	from	women	948:952	arg1	data					934:937	data	934:937	data from 113 women in Mexico City	934:967	METHODS We analyzed data from 113 women in Mexico City and compared the amount of IgA subclasses in colostrum against three antigens: two from vaccine protocols (tetanus toxoid and pneumococcal polysaccharides) and lipopolysaccharide, a ubiquitous antigen in the gastrointestinal tract.
32284627	7	65	dep	protocols	1065:1073	arg1	toxoid					1084:1089	tetanus toxoid	1076:1089	tetanus toxoid	1076:1089	METHODS We analyzed data from 113 women in Mexico City and compared the amount of IgA subclasses in colostrum against three antigens: two from vaccine protocols (tetanus toxoid and pneumococcal polysaccharides) and lipopolysaccharide, a ubiquitous antigen in the gastrointestinal tract.
32284627	7	65	dep	protocols	1065:1073	arg1	polysaccharides					1108:1122	pneumococcal polysaccharides	1095:1122	pneumococcal polysaccharides	1095:1122	METHODS We analyzed data from 113 women in Mexico City and compared the amount of IgA subclasses in colostrum against three antigens: two from vaccine protocols (tetanus toxoid and pneumococcal polysaccharides) and lipopolysaccharide, a ubiquitous antigen in the gastrointestinal tract.
32284627	6	66	theme	stimulation	806:816	arg1	effect					786:791	the effect	782:791	the effect of antigenic stimulation during pregnancy on the concentrations of specific IgA1 and IgA2 subclasses in human colostrum	782:911	We studied the effect of antigenic stimulation during pregnancy on the concentrations of specific IgA1 and IgA2 subclasses in human colostrum.
32284627	1	67	theme	vaccine	235:241	arg1	protocols					243:251	vaccine protocols	235:251	vaccine protocols during pregnancy	235:268	BACKGROUND Several studies have evaluated the effect of infectious diseases and vaccine protocols during pregnancy on maternal milk immunoglobulin (Ig) levels, to understand the protection conferred by lactation on newborns.
32284627	5	68	theme	antigens	761:768	arg1	composition					742:752	the chemical composition	729:752	the chemical composition of the antigens	729:768	Total IgA levels in maternal milk vary after antigen stimulation and have differential affinities in function of the chemical composition of the antigens.
32284627	6	69	from	effect	786:791	arg1	concentrations					842:855	the concentrations	838:855	the concentrations of specific IgA1 and IgA2 subclasses in human colostrum	838:911	We studied the effect of antigenic stimulation during pregnancy on the concentrations of specific IgA1 and IgA2 subclasses in human colostrum.
32284627	8	70	theme	sharp	1323:1327	arg1	contrast					1329:1336	sharp contrast	1323:1336	sharp contrast	1323:1336	RESULTS In agreement with the previous reports, we showed that IgA1 from colostrum mainly recognized protein antigens; in sharp contrast, IgA2 was mostly directed against polysaccharide antigens.
32284627	1	71	theme	protocols	243:251	arg1	effect					201:206	the effect	197:206	the effect of infectious diseases and vaccine protocols during pregnancy on maternal milk immunoglobulin (Ig) levels	197:312	BACKGROUND Several studies have evaluated the effect of infectious diseases and vaccine protocols during pregnancy on maternal milk immunoglobulin (Ig) levels, to understand the protection conferred by lactation on newborns.
32284627	0	72	theme	IGA2	66:69	arg1	SUBCLASSES					71:80	SPECIFIC IGA1 AND IGA2 SUBCLASSES	48:80	SPECIFIC IGA1 AND IGA2 SUBCLASSES	48:80	ANTIGENIC STIMULATION DURING PREGNANCY MODIFIES SPECIFIC IGA1 AND IGA2 SUBCLASSES IN HUMAN COLOSTRUM ACCORDING TO THE CHEMICAL COMPOSITION OF THE ANTIGEN.
32284627	10	73	theme	chemical	1622:1629	arg1	composition					1631:1641	the chemical composition	1618:1641	the chemical composition of the antigen	1618:1656	CONCLUSIONS Antigen interaction during pregnancy increased the amount of specific IgA subclasses, depending on the chemical composition of the antigen.
32375979	8	0	theme	S-1144T	1036:1042	arg1	content					1018:1024	The DNA G+C content	1006:1024	The DNA G+C content of strain S-1144T	1006:1042	The DNA G+C content of strain S-1144T was 73.5 mol%.
32375979	8	0	theme	S-1144T	1036:1042	arg1	%					1056:1056	73.5 mol%	1048:1056	73.5 mol%	1048:1056	The DNA G+C content of strain S-1144T was 73.5 mol%.
32375979	12	1	theme	dongxiaopingii	1625:1638	arg1	sp					1640:1641	the name Nocardioides dongxiaopingii sp	1603:1641	the name Nocardioides dongxiaopingii sp	1603:1641	On the basis of obtained data, strain S-1144T represented a novel species of the genus Nocardioides, for which the name Nocardioides dongxiaopingii sp.
32375979	11	2	theme	 1	1484:1485	arg1	ω9c					1487:1489	 1 ω9c	1484:1489	 1 ω9c	1484:1489	The major cellular fatty acids were iso-C16 : 0, C17 : 1 ω8c, C17 : 0 and C18 : 1 ω9c.
32375979	8	3	theme	73.5 mol	1048:1055	arg1	content					1018:1024	The DNA G+C content	1006:1024	The DNA G+C content of strain S-1144T	1006:1042	The DNA G+C content of strain S-1144T was 73.5 mol%.
32375979	8	3	theme	73.5 mol	1048:1055	arg1	%					1056:1056	73.5 mol%	1048:1056	73.5 mol%	1048:1056	The DNA G+C content of strain S-1144T was 73.5 mol%.
32375979	10	4	theme	lipid	1224:1228	arg1	profile					1230:1236	The polar lipid profile	1214:1236	The polar lipid profile of strain S-1144T	1214:1254	The polar lipid profile of strain S-1144T consisted of diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, one unidentified glycolipid and one unidentified lipid.
32375979	6	5	theme	strain	579:584	arg1	S-1144T					586:592	strain S-1144T	579:592	strain S-1144T	579:592	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	9	6	from	quinone	1100:1106	arg1	peptidoglycan					1199:1211	the cell-wall peptidoglycan	1185:1211	the cell-wall peptidoglycan	1185:1211	MK-8(H4) was the predominant respiratory quinone (96.0 %) and llLL-2,6-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
32375979	4	7	theme	2A	369:370	arg1	agar					372:375	Reasoner's 2A agar	358:375	Reasoner's 2A agar	358:375	Colonies on Reasoner's 2A agar were light yellow, circular, shiny, smooth and convex after 2 days of incubation.
32375979	7	8	theme	above	981:985	arg1	strains					997:1003	the above reference strains	977:1003	the above reference strains	977:1003	The genome of strain S-1144T showed less than 70 % digital DNA-DNA hybridization and < 95-96 % average nucleotide identity values to the above reference strains.
32375979	6	9	theme	DSM	729:731	arg1	103718T					733:739	Nocardioides litoris DSM 103718T	708:739	Nocardioides litoris DSM 103718T (98.4 %)	708:748	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	6	9	theme	DSM	729:731	arg1	%					747:747	98.4 %	742:747	98.4 %	742:747	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	9	10	from	acid	1177:1180	arg1	peptidoglycan					1199:1211	the cell-wall peptidoglycan	1185:1211	the cell-wall peptidoglycan	1185:1211	MK-8(H4) was the predominant respiratory quinone (96.0 %) and llLL-2,6-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
32375979	12	11	theme	genus	1573:1577	arg1	Nocardioides					1579:1590	the genus Nocardioides	1569:1590	the genus Nocardioides	1569:1590	On the basis of obtained data, strain S-1144T represented a novel species of the genus Nocardioides, for which the name Nocardioides dongxiaopingii sp.
32375979	5	12	theme	%	515:515	arg1	NaCl					523:526	0 % (w/v) NaCl	513:526	0 % (w/v) NaCl	513:526	The isolates grew optimally at 25 °C, pH 7.5 and with 0 % (w/v) NaCl.
32375979	7	13	theme	identity	958:965	arg1	values					967:972	less than 70 % digital DNA-DNA hybridization and < 95-96 % average nucleotide identity values	880:972	less than 70 % digital DNA-DNA hybridization and < 95-96 % average nucleotide identity values	880:972	The genome of strain S-1144T showed less than 70 % digital DNA-DNA hybridization and < 95-96 % average nucleotide identity values to the above reference strains.
32375979	2	14	theme	Lamiophlomis	226:237	arg1	rotata					239:244	Lamiophlomis rotata	226:244	Lamiophlomis rotata	226:244	Two novel Gram-stain-positive, irregular rod-shaped actinomycetes, S-1144T and 4053, were isolated from leaves of Lamiophlomis rotata on the Qinghai-Tibet Plateau, PR China.
32375979	7	15	theme	hybridization	911:923	arg1	values					967:972	less than 70 % digital DNA-DNA hybridization and < 95-96 % average nucleotide identity values	880:972	less than 70 % digital DNA-DNA hybridization and < 95-96 % average nucleotide identity values	880:972	The genome of strain S-1144T showed less than 70 % digital DNA-DNA hybridization and < 95-96 % average nucleotide identity values to the above reference strains.
32375979	14	16	theme	4.7568T=JCM	1697:1707	arg1	S-1144T					1681:1687	S-1144T	1681:1687	S-1144T (=CGMCC 4.7568T=JCM 33469T)	1681:1715	The type strain is S-1144T (=CGMCC 4.7568T=JCM 33469T).
32375979	14	16	theme	4.7568T=JCM	1697:1707	arg1	33469T					1709:1714	=CGMCC 4.7568T=JCM 33469T	1690:1714	=CGMCC 4.7568T=JCM 33469T	1690:1714	The type strain is S-1144T (=CGMCC 4.7568T=JCM 33469T).
32375979	7	17	theme	 average	938:945	arg1	identity					958:965	< 95-96 % average nucleotide identity	929:965	< 95-96 % average nucleotide identity	929:965	The genome of strain S-1144T showed less than 70 % digital DNA-DNA hybridization and < 95-96 % average nucleotide identity values to the above reference strains.
32375979	7	18	theme	digital	895:901	arg1	hybridization					911:923	digital DNA-DNA hybridization	895:923	digital DNA-DNA hybridization	895:923	The genome of strain S-1144T showed less than 70 % digital DNA-DNA hybridization and < 95-96 % average nucleotide identity values to the above reference strains.
32375979	7	19	theme	95-96 	931:936	arg1	identity					958:965	< 95-96 % average nucleotide identity	929:965	< 95-96 % average nucleotide identity	929:965	The genome of strain S-1144T showed less than 70 % digital DNA-DNA hybridization and < 95-96 % average nucleotide identity values to the above reference strains.
32375979	9	20	theme	llLL-2,6-diaminopimelic	1121:1143	arg1	acid					1177:1180	the diagnostic diamino acid	1154:1180	the diagnostic diamino acid in the cell-wall peptidoglycan	1154:1211	MK-8(H4) was the predominant respiratory quinone (96.0 %) and llLL-2,6-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
32375979	9	20	theme	llLL-2,6-diaminopimelic	1121:1143	arg1	acid					1145:1148	llLL-2,6-diaminopimelic acid	1121:1148	llLL-2,6-diaminopimelic acid	1121:1148	MK-8(H4) was the predominant respiratory quinone (96.0 %) and llLL-2,6-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
32375979	2	21	theme	Qinghai-Tibet	253:265	arg1	China					279:283	PR China	276:283	PR China	276:283	Two novel Gram-stain-positive, irregular rod-shaped actinomycetes, S-1144T and 4053, were isolated from leaves of Lamiophlomis rotata on the Qinghai-Tibet Plateau, PR China.
32375979	2	21	theme	Qinghai-Tibet	253:265	arg1	Plateau					267:273	the Qinghai-Tibet Plateau	249:273	the Qinghai-Tibet Plateau	249:273	Two novel Gram-stain-positive, irregular rod-shaped actinomycetes, S-1144T and 4053, were isolated from leaves of Lamiophlomis rotata on the Qinghai-Tibet Plateau, PR China.
32375979	14	22	theme	type	1666:1669	arg1	S-1144T					1681:1687	S-1144T	1681:1687	S-1144T (=CGMCC 4.7568T=JCM 33469T)	1681:1715	The type strain is S-1144T (=CGMCC 4.7568T=JCM 33469T).
32375979	14	22	theme	type	1666:1669	arg1	strain					1671:1676	The type strain	1662:1676	The type strain	1662:1676	The type strain is S-1144T (=CGMCC 4.7568T=JCM 33469T).
32375979	9	23	theme	diagnostic	1158:1167	arg1	acid					1177:1180	the diagnostic diamino acid	1154:1180	the diagnostic diamino acid in the cell-wall peptidoglycan	1154:1211	MK-8(H4) was the predominant respiratory quinone (96.0 %) and llLL-2,6-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
32375979	9	23	theme	diagnostic	1158:1167	arg1	acid					1145:1148	llLL-2,6-diaminopimelic acid	1121:1148	llLL-2,6-diaminopimelic acid	1121:1148	MK-8(H4) was the predominant respiratory quinone (96.0 %) and llLL-2,6-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
32375979	7	24	theme	S-1144T	865:871	arg1	genome					848:853	The genome	844:853	The genome of strain S-1144T	844:871	The genome of strain S-1144T showed less than 70 % digital DNA-DNA hybridization and < 95-96 % average nucleotide identity values to the above reference strains.
32375979	6	25	theme	analyses	555:562	arg1	results					533:539	The results	529:539	The results of polyphasic analyses	529:562	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	4	26	theme	incubation	447:456	arg1	2 days					437:442	2 days	437:442	2 days of incubation	437:456	Colonies on Reasoner's 2A agar were light yellow, circular, shiny, smooth and convex after 2 days of incubation.
32375979	12	27	theme	obtained	1508:1515	arg1	data					1517:1520	obtained data	1508:1520	obtained data	1508:1520	On the basis of obtained data, strain S-1144T represented a novel species of the genus Nocardioides, for which the name Nocardioides dongxiaopingii sp.
32375979	6	28	theme	close	637:641	arg1	neighbours					656:665	its close phylogenetic neighbours	633:665	its close phylogenetic neighbours (16S rRNA gene sequence similarity)	633:701	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	6	28	theme	close	637:641	arg1	similarity					691:700	16S rRNA gene sequence similarity	668:700	16S rRNA gene sequence similarity	668:700	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	6	29	dep	Nocardioides	751:762	arg1	rubriscoriae					764:775	rubriscoriae	764:775	rubriscoriae	764:775	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	12	30	dep	data	1517:1520	arg1	basis					1499:1503	basis	1499:1503	basis	1499:1503	On the basis of obtained data, strain S-1144T represented a novel species of the genus Nocardioides, for which the name Nocardioides dongxiaopingii sp.
32375979	12	30	dep	data	1517:1520	arg1	the					1495:1497	the	1495:1497	the	1495:1497	On the basis of obtained data, strain S-1144T represented a novel species of the genus Nocardioides, for which the name Nocardioides dongxiaopingii sp.
32375979	6	31	theme	genus	610:614	arg1	Nocardioides					616:627	the genus Nocardioides	606:627	the genus Nocardioides	606:627	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	7	32	dep	%	893:893	arg1	70 					890:892	70 	890:892	70 	890:892	The genome of strain S-1144T showed less than 70 % digital DNA-DNA hybridization and < 95-96 % average nucleotide identity values to the above reference strains.
32375979	8	33	theme	DNA	1010:1012	arg1	content					1018:1024	The DNA G+C content	1006:1024	The DNA G+C content of strain S-1144T	1006:1042	The DNA G+C content of strain S-1144T was 73.5 mol%.
32375979	8	33	theme	DNA	1010:1012	arg1	%					1056:1056	73.5 mol%	1048:1056	73.5 mol%	1048:1056	The DNA G+C content of strain S-1144T was 73.5 mol%.
32375979	0	34	dep	sp	28:29	arg1	Nocardioides					0:11	Nocardioides	0:11	Nocardioides	0:11	Nocardioides dongxiaopingii sp.
32375979	12	35	theme	Nocardioides	1612:1623	arg1	sp					1640:1641	the name Nocardioides dongxiaopingii sp	1603:1641	the name Nocardioides dongxiaopingii sp	1603:1641	On the basis of obtained data, strain S-1144T represented a novel species of the genus Nocardioides, for which the name Nocardioides dongxiaopingii sp.
32375979	6	36	theme	gene	677:680	arg1	neighbours					656:665	its close phylogenetic neighbours	633:665	its close phylogenetic neighbours (16S rRNA gene sequence similarity)	633:701	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	6	36	theme	gene	677:680	arg1	similarity					691:700	16S rRNA gene sequence similarity	668:700	16S rRNA gene sequence similarity	668:700	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	7	37	dep	values	967:972	arg1	%					893:893	%	893:893	%	893:893	The genome of strain S-1144T showed less than 70 % digital DNA-DNA hybridization and < 95-96 % average nucleotide identity values to the above reference strains.
32375979	2	38	theme	novel	116:120	arg1	4053					191:194	4053	191:194	4053	191:194	Two novel Gram-stain-positive, irregular rod-shaped actinomycetes, S-1144T and 4053, were isolated from leaves of Lamiophlomis rotata on the Qinghai-Tibet Plateau, PR China.
32375979	2	38	theme	novel	116:120	arg1	S-1144T					179:185	S-1144T	179:185	S-1144T	179:185	Two novel Gram-stain-positive, irregular rod-shaped actinomycetes, S-1144T and 4053, were isolated from leaves of Lamiophlomis rotata on the Qinghai-Tibet Plateau, PR China.
32375979	2	38	theme	novel	116:120	arg1	actinomycetes					164:176	Two novel Gram-stain-positive, irregular rod-shaped actinomycetes	112:176	Two novel Gram-stain-positive, irregular rod-shaped actinomycetes	112:176	Two novel Gram-stain-positive, irregular rod-shaped actinomycetes, S-1144T and 4053, were isolated from leaves of Lamiophlomis rotata on the Qinghai-Tibet Plateau, PR China.
32375979	6	39	theme	16S	668:670	arg1	neighbours					656:665	its close phylogenetic neighbours	633:665	its close phylogenetic neighbours (16S rRNA gene sequence similarity)	633:701	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	6	39	theme	16S	668:670	arg1	similarity					691:700	16S rRNA gene sequence similarity	668:700	16S rRNA gene sequence similarity	668:700	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	1	40	from	rotata	75:80	arg1	Plateau					103:109	the Qinghai-Tibet Plateau	85:109	the Qinghai-Tibet Plateau	85:109	nov., isolated from leaves of Lamiophlomis rotata on the Qinghai-Tibet Plateau.
32375979	8	41	theme	strain	1029:1034	arg1	S-1144T					1036:1042	strain S-1144T	1029:1042	strain S-1144T	1029:1042	The DNA G+C content of strain S-1144T was 73.5 mol%.
32375979	6	42	theme	DSM	823:825	arg1	11054T					827:832	Nocardioides plantarum DSM 11054T	800:832	Nocardioides plantarum DSM 11054T (97.8 %)	800:841	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	6	42	theme	DSM	823:825	arg1	%					840:840	97.8 %	835:840	97.8 %	835:840	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	5	43	dep	%	515:515	arg1	w/v					518:520	w/v	518:520	w/v	518:520	The isolates grew optimally at 25 °C, pH 7.5 and with 0 % (w/v) NaCl.
32375979	2	44	theme	rod-shaped	153:162	arg1	4053					191:194	4053	191:194	4053	191:194	Two novel Gram-stain-positive, irregular rod-shaped actinomycetes, S-1144T and 4053, were isolated from leaves of Lamiophlomis rotata on the Qinghai-Tibet Plateau, PR China.
32375979	2	44	theme	rod-shaped	153:162	arg1	S-1144T					179:185	S-1144T	179:185	S-1144T	179:185	Two novel Gram-stain-positive, irregular rod-shaped actinomycetes, S-1144T and 4053, were isolated from leaves of Lamiophlomis rotata on the Qinghai-Tibet Plateau, PR China.
32375979	2	44	theme	rod-shaped	153:162	arg1	actinomycetes					164:176	Two novel Gram-stain-positive, irregular rod-shaped actinomycetes	112:176	Two novel Gram-stain-positive, irregular rod-shaped actinomycetes	112:176	Two novel Gram-stain-positive, irregular rod-shaped actinomycetes, S-1144T and 4053, were isolated from leaves of Lamiophlomis rotata on the Qinghai-Tibet Plateau, PR China.
32375979	7	45	theme	reference	987:995	arg1	strains					997:1003	the above reference strains	977:1003	the above reference strains	977:1003	The genome of strain S-1144T showed less than 70 % digital DNA-DNA hybridization and < 95-96 % average nucleotide identity values to the above reference strains.
32375979	10	46	theme	S-1144T	1248:1254	arg1	profile					1230:1236	The polar lipid profile	1214:1236	The polar lipid profile of strain S-1144T	1214:1254	The polar lipid profile of strain S-1144T consisted of diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, one unidentified glycolipid and one unidentified lipid.
32375979	2	47	theme	PR	276:277	arg1	China					279:283	PR China	276:283	PR China	276:283	Two novel Gram-stain-positive, irregular rod-shaped actinomycetes, S-1144T and 4053, were isolated from leaves of Lamiophlomis rotata on the Qinghai-Tibet Plateau, PR China.
32375979	2	47	theme	PR	276:277	arg1	Plateau					267:273	the Qinghai-Tibet Plateau	249:273	the Qinghai-Tibet Plateau	249:273	Two novel Gram-stain-positive, irregular rod-shaped actinomycetes, S-1144T and 4053, were isolated from leaves of Lamiophlomis rotata on the Qinghai-Tibet Plateau, PR China.
32375979	6	48	theme	Nocardioides	800:811	arg1	11054T					827:832	Nocardioides plantarum DSM 11054T	800:832	Nocardioides plantarum DSM 11054T (97.8 %)	800:841	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	6	48	theme	Nocardioides	800:811	arg1	%					840:840	97.8 %	835:840	97.8 %	835:840	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	6	49	theme	polyphasic	544:553	arg1	analyses					555:562	polyphasic analyses	544:562	polyphasic analyses	544:562	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	12	50	theme	Nocardioides	1579:1590	arg1	species					1558:1564	a novel species	1550:1564	a novel species	1550:1564	On the basis of obtained data, strain S-1144T represented a novel species of the genus Nocardioides, for which the name Nocardioides dongxiaopingii sp.
32375979	9	51	theme	predominant	1076:1086	arg1	quinone					1100:1106	the predominant respiratory quinone	1072:1106	the predominant respiratory quinone	1072:1106	MK-8(H4) was the predominant respiratory quinone (96.0 %) and llLL-2,6-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
32375979	9	51	theme	predominant	1076:1086	arg1	MK-8					1059:1062	MK-8	1059:1062	MK-8(H4)	1059:1066	MK-8(H4) was the predominant respiratory quinone (96.0 %) and llLL-2,6-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
32375979	6	52	theme	litoris	721:727	arg1	103718T					733:739	Nocardioides litoris DSM 103718T	708:739	Nocardioides litoris DSM 103718T (98.4 %)	708:748	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	6	52	theme	litoris	721:727	arg1	%					747:747	98.4 %	742:747	98.4 %	742:747	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	7	53	theme	nucleotide	947:956	arg1	identity					958:965	< 95-96 % average nucleotide identity	929:965	< 95-96 % average nucleotide identity	929:965	The genome of strain S-1144T showed less than 70 % digital DNA-DNA hybridization and < 95-96 % average nucleotide identity values to the above reference strains.
32375979	7	54	theme	%	937:937	arg1	identity					958:965	< 95-96 % average nucleotide identity	929:965	< 95-96 % average nucleotide identity	929:965	The genome of strain S-1144T showed less than 70 % digital DNA-DNA hybridization and < 95-96 % average nucleotide identity values to the above reference strains.
32375979	5	55	theme	0 	513:514	arg1	%					515:515	%	515:515	%	515:515	The isolates grew optimally at 25 °C, pH 7.5 and with 0 % (w/v) NaCl.
32375979	14	56	theme	=CGMCC	1690:1695	arg1	S-1144T					1681:1687	S-1144T	1681:1687	S-1144T (=CGMCC 4.7568T=JCM 33469T)	1681:1715	The type strain is S-1144T (=CGMCC 4.7568T=JCM 33469T).
32375979	14	56	theme	=CGMCC	1690:1695	arg1	33469T					1709:1714	=CGMCC 4.7568T=JCM 33469T	1690:1714	=CGMCC 4.7568T=JCM 33469T	1690:1714	The type strain is S-1144T (=CGMCC 4.7568T=JCM 33469T).
32375979	9	57	theme	respiratory	1088:1098	arg1	quinone					1100:1106	the predominant respiratory quinone	1072:1106	the predominant respiratory quinone	1072:1106	MK-8(H4) was the predominant respiratory quinone (96.0 %) and llLL-2,6-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
32375979	9	57	theme	respiratory	1088:1098	arg1	MK-8					1059:1062	MK-8	1059:1062	MK-8(H4)	1059:1066	MK-8(H4) was the predominant respiratory quinone (96.0 %) and llLL-2,6-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
32375979	7	58	theme	<	929:929	arg1	95-96 					931:936	95-96 	931:936	95-96 	931:936	The genome of strain S-1144T showed less than 70 % digital DNA-DNA hybridization and < 95-96 % average nucleotide identity values to the above reference strains.
32375979	7	59	theme	DNA-DNA	903:909	arg1	hybridization					911:923	digital DNA-DNA hybridization	895:923	digital DNA-DNA hybridization	895:923	The genome of strain S-1144T showed less than 70 % digital DNA-DNA hybridization and < 95-96 % average nucleotide identity values to the above reference strains.
32375979	11	60	theme	cellular	1415:1422	arg1	iso-C16 					1441:1448	iso-C16 	1441:1448	iso-C16 	1441:1448	The major cellular fatty acids were iso-C16 : 0, C17 : 1 ω8c, C17 : 0 and C18 : 1 ω9c.
32375979	11	60	theme	cellular	1415:1422	arg1	acids					1430:1434	The major cellular fatty acids	1405:1434	The major cellular fatty acids	1405:1434	The major cellular fatty acids were iso-C16 : 0, C17 : 1 ω8c, C17 : 0 and C18 : 1 ω9c.
32375979	12	61	theme	novel	1552:1556	arg1	species					1558:1564	a novel species	1550:1564	a novel species	1550:1564	On the basis of obtained data, strain S-1144T represented a novel species of the genus Nocardioides, for which the name Nocardioides dongxiaopingii sp.
32375979	11	62	theme	fatty	1424:1428	arg1	iso-C16 					1441:1448	iso-C16 	1441:1448	iso-C16 	1441:1448	The major cellular fatty acids were iso-C16 : 0, C17 : 1 ω8c, C17 : 0 and C18 : 1 ω9c.
32375979	11	62	theme	fatty	1424:1428	arg1	acids					1430:1434	The major cellular fatty acids	1405:1434	The major cellular fatty acids	1405:1434	The major cellular fatty acids were iso-C16 : 0, C17 : 1 ω8c, C17 : 0 and C18 : 1 ω9c.
32375979	11	63	theme	major	1409:1413	arg1	iso-C16 					1441:1448	iso-C16 	1441:1448	iso-C16 	1441:1448	The major cellular fatty acids were iso-C16 : 0, C17 : 1 ω8c, C17 : 0 and C18 : 1 ω9c.
32375979	11	63	theme	major	1409:1413	arg1	acids					1430:1434	The major cellular fatty acids	1405:1434	The major cellular fatty acids	1405:1434	The major cellular fatty acids were iso-C16 : 0, C17 : 1 ω8c, C17 : 0 and C18 : 1 ω9c.
32375979	9	64	theme	diamino	1169:1175	arg1	acid					1177:1180	the diagnostic diamino acid	1154:1180	the diagnostic diamino acid in the cell-wall peptidoglycan	1154:1211	MK-8(H4) was the predominant respiratory quinone (96.0 %) and llLL-2,6-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
32375979	9	64	theme	diamino	1169:1175	arg1	acid					1145:1148	llLL-2,6-diaminopimelic acid	1121:1148	llLL-2,6-diaminopimelic acid	1121:1148	MK-8(H4) was the predominant respiratory quinone (96.0 %) and llLL-2,6-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
32375979	12	65	theme	strain	1523:1528	arg1	S-1144T					1530:1536	strain S-1144T	1523:1536	strain S-1144T	1523:1536	On the basis of obtained data, strain S-1144T represented a novel species of the genus Nocardioides, for which the name Nocardioides dongxiaopingii sp.
32375979	6	66	theme	sequence	682:689	arg1	neighbours					656:665	its close phylogenetic neighbours	633:665	its close phylogenetic neighbours (16S rRNA gene sequence similarity)	633:701	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	6	66	theme	sequence	682:689	arg1	similarity					691:700	16S rRNA gene sequence similarity	668:700	16S rRNA gene sequence similarity	668:700	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	10	67	theme	unidentified	1321:1332	arg1	phospholipids					1334:1346	three unidentified phospholipids	1315:1346	three unidentified phospholipids	1315:1346	The polar lipid profile of strain S-1144T consisted of diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, one unidentified glycolipid and one unidentified lipid.
32375979	7	68	theme	strain	858:863	arg1	S-1144T					865:871	strain S-1144T	858:871	strain S-1144T	858:871	The genome of strain S-1144T showed less than 70 % digital DNA-DNA hybridization and < 95-96 % average nucleotide identity values to the above reference strains.
32375979	1	69	theme	Lamiophlomis	62:73	arg1	rotata					75:80	Lamiophlomis rotata	62:80	Lamiophlomis rotata on the Qinghai-Tibet Plateau	62:109	nov., isolated from leaves of Lamiophlomis rotata on the Qinghai-Tibet Plateau.
32375979	2	70	attach	isolated	202:209	arg2	actinomycetes					164:176	Two novel Gram-stain-positive, irregular rod-shaped actinomycetes	112:176	Two novel Gram-stain-positive, irregular rod-shaped actinomycetes	112:176	Two novel Gram-stain-positive, irregular rod-shaped actinomycetes, S-1144T and 4053, were isolated from leaves of Lamiophlomis rotata on the Qinghai-Tibet Plateau, PR China.
32375979	2	70	attach	isolated	202:209	arg1	leaves					216:221	leaves	216:221	leaves	216:221	Two novel Gram-stain-positive, irregular rod-shaped actinomycetes, S-1144T and 4053, were isolated from leaves of Lamiophlomis rotata on the Qinghai-Tibet Plateau, PR China.
32375979	2	70	attach	isolated	202:209	arg2	4053					191:194	4053	191:194	4053	191:194	Two novel Gram-stain-positive, irregular rod-shaped actinomycetes, S-1144T and 4053, were isolated from leaves of Lamiophlomis rotata on the Qinghai-Tibet Plateau, PR China.
32375979	2	70	attach	isolated	202:209	arg2	S-1144T					179:185	S-1144T	179:185	S-1144T	179:185	Two novel Gram-stain-positive, irregular rod-shaped actinomycetes, S-1144T and 4053, were isolated from leaves of Lamiophlomis rotata on the Qinghai-Tibet Plateau, PR China.
32375979	6	71	theme	phylogenetic	643:654	arg1	neighbours					656:665	its close phylogenetic neighbours	633:665	its close phylogenetic neighbours (16S rRNA gene sequence similarity)	633:701	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	6	71	theme	phylogenetic	643:654	arg1	similarity					691:700	16S rRNA gene sequence similarity	668:700	16S rRNA gene sequence similarity	668:700	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	0	72	theme	dongxiaopingii	13:26	arg1	sp					28:29	dongxiaopingii sp	13:29	dongxiaopingii sp	13:29	Nocardioides dongxiaopingii sp.
32375979	9	73	theme	cell-wall	1189:1197	arg1	peptidoglycan					1199:1211	the cell-wall peptidoglycan	1185:1211	the cell-wall peptidoglycan	1185:1211	MK-8(H4) was the predominant respiratory quinone (96.0 %) and llLL-2,6-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
32375979	10	74	theme	unidentified	1353:1364	arg1	glycolipid					1366:1375	one unidentified glycolipid	1349:1375	one unidentified glycolipid	1349:1375	The polar lipid profile of strain S-1144T consisted of diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, one unidentified glycolipid and one unidentified lipid.
32375979	11	75	dep	ω9c	1487:1489	arg1	C17 					1467:1470	C17 	1467:1470	C17 	1467:1470	The major cellular fatty acids were iso-C16 : 0, C17 : 1 ω8c, C17 : 0 and C18 : 1 ω9c.
32375979	11	75	dep	ω9c	1487:1489	arg1	C17 					1454:1457	C17 	1454:1457	C17 	1454:1457	The major cellular fatty acids were iso-C16 : 0, C17 : 1 ω8c, C17 : 0 and C18 : 1 ω9c.
32375979	11	75	dep	ω9c	1487:1489	arg1	C18 					1479:1482	C18 	1479:1482	C18 	1479:1482	The major cellular fatty acids were iso-C16 : 0, C17 : 1 ω8c, C17 : 0 and C18 : 1 ω9c.
32375979	11	75	dep	ω9c	1487:1489	arg1	 0					1472:1473	 0	1472:1473	 0	1472:1473	The major cellular fatty acids were iso-C16 : 0, C17 : 1 ω8c, C17 : 0 and C18 : 1 ω9c.
32375979	1	76	theme	Qinghai-Tibet	89:101	arg1	Plateau					103:109	the Qinghai-Tibet Plateau	85:109	the Qinghai-Tibet Plateau	85:109	nov., isolated from leaves of Lamiophlomis rotata on the Qinghai-Tibet Plateau.
32375979	10	77	theme	unidentified	1385:1396	arg1	lipid					1398:1402	one unidentified lipid	1381:1402	one unidentified lipid	1381:1402	The polar lipid profile of strain S-1144T consisted of diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, one unidentified glycolipid and one unidentified lipid.
32375979	6	78	theme	Nocardioides	708:719	arg1	103718T					733:739	Nocardioides litoris DSM 103718T	708:739	Nocardioides litoris DSM 103718T (98.4 %)	708:748	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	6	78	theme	Nocardioides	708:719	arg1	%					747:747	98.4 %	742:747	98.4 %	742:747	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	2	79	dep	Gram-stain-positive	122:140	arg1	irregular					143:151	irregular	143:151	irregular	143:151	Two novel Gram-stain-positive, irregular rod-shaped actinomycetes, S-1144T and 4053, were isolated from leaves of Lamiophlomis rotata on the Qinghai-Tibet Plateau, PR China.
32375979	8	80	theme	G+C	1014:1016	arg1	content					1018:1024	The DNA G+C content	1006:1024	The DNA G+C content of strain S-1144T	1006:1042	The DNA G+C content of strain S-1144T was 73.5 mol%.
32375979	8	80	theme	G+C	1014:1016	arg1	%					1056:1056	73.5 mol%	1048:1056	73.5 mol%	1048:1056	The DNA G+C content of strain S-1144T was 73.5 mol%.
32375979	6	81	theme	DSM	777:779	arg1	23986T					781:786	Nocardioides rubriscoriae DSM 23986T	751:786	Nocardioides rubriscoriae DSM 23986T (98.2%)	751:794	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	6	81	theme	DSM	777:779	arg1	%					793:793	98.2%	789:793	98.2%	789:793	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	6	82	dep	Nocardioides	800:811	arg1	plantarum					813:821	plantarum	813:821	plantarum	813:821	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	10	83	theme	strain	1241:1246	arg1	S-1144T					1248:1254	strain S-1144T	1241:1254	strain S-1144T	1241:1254	The polar lipid profile of strain S-1144T consisted of diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, one unidentified glycolipid and one unidentified lipid.
32375979	10	84	theme	polar	1218:1222	arg1	profile					1230:1236	The polar lipid profile	1214:1236	The polar lipid profile of strain S-1144T	1214:1254	The polar lipid profile of strain S-1144T consisted of diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, one unidentified glycolipid and one unidentified lipid.
32375979	6	85	theme	Nocardioides	751:762	arg1	23986T					781:786	Nocardioides rubriscoriae DSM 23986T	751:786	Nocardioides rubriscoriae DSM 23986T (98.2%)	751:794	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	6	85	theme	Nocardioides	751:762	arg1	%					793:793	98.2%	789:793	98.2%	789:793	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	12	86	theme	name	1607:1610	arg1	sp					1640:1641	the name Nocardioides dongxiaopingii sp	1603:1641	the name Nocardioides dongxiaopingii sp	1603:1641	On the basis of obtained data, strain S-1144T represented a novel species of the genus Nocardioides, for which the name Nocardioides dongxiaopingii sp.
32375979	9	87	dep	quinone	1100:1106	arg1	%					1114:1114	96.0 %	1109:1114	96.0 %	1109:1114	MK-8(H4) was the predominant respiratory quinone (96.0 %) and llLL-2,6-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan.
32375979	2	88	theme	Gram-stain-positive	122:140	arg1	4053					191:194	4053	191:194	4053	191:194	Two novel Gram-stain-positive, irregular rod-shaped actinomycetes, S-1144T and 4053, were isolated from leaves of Lamiophlomis rotata on the Qinghai-Tibet Plateau, PR China.
32375979	2	88	theme	Gram-stain-positive	122:140	arg1	S-1144T					179:185	S-1144T	179:185	S-1144T	179:185	Two novel Gram-stain-positive, irregular rod-shaped actinomycetes, S-1144T and 4053, were isolated from leaves of Lamiophlomis rotata on the Qinghai-Tibet Plateau, PR China.
32375979	2	88	theme	Gram-stain-positive	122:140	arg1	actinomycetes					164:176	Two novel Gram-stain-positive, irregular rod-shaped actinomycetes	112:176	Two novel Gram-stain-positive, irregular rod-shaped actinomycetes	112:176	Two novel Gram-stain-positive, irregular rod-shaped actinomycetes, S-1144T and 4053, were isolated from leaves of Lamiophlomis rotata on the Qinghai-Tibet Plateau, PR China.
32375979	6	89	theme	rRNA	672:675	arg1	neighbours					656:665	its close phylogenetic neighbours	633:665	its close phylogenetic neighbours (16S rRNA gene sequence similarity)	633:701	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
32375979	6	89	theme	rRNA	672:675	arg1	similarity					691:700	16S rRNA gene sequence similarity	668:700	16S rRNA gene sequence similarity	668:700	The results of polyphasic analyses indicated that strain S-1144T belonged to the genus Nocardioides and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were Nocardioides litoris DSM 103718T (98.4 %), Nocardioides rubriscoriae DSM 23986T (98.2%) and Nocardioides plantarum DSM 11054T (97.8 %).
33625539	3	0	theme	genus	300:304	arg1	WN066T					273:278	WN066T	273:278	WN066T	273:278	Phylogenetic analysis based on 16S rRNA gene sequence indicated WN066T was a member of the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%), Bacillus cucumis CCM 8651T (98.8%), Bacillus bataviensis DSM 15601T (98.7%) and Bacillus niacini DSM 2923T (98.7%).
33625539	3	0	theme	genus	300:304	arg1	member					286:291	a member	284:291	a member of the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%)	284:384	Phylogenetic analysis based on 16S rRNA gene sequence indicated WN066T was a member of the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%), Bacillus cucumis CCM 8651T (98.8%), Bacillus bataviensis DSM 15601T (98.7%) and Bacillus niacini DSM 2923T (98.7%).
33625539	2	1	dep	Gram-staining-positive	69:90	arg1	aerobic					112:118	aerobic	112:118	aerobic	112:118	A Gram-staining-positive, endospore-forming, aerobic strain, designed WN066T, was isolated from saline-alkali wetland soil of Tianjin, China.
33625539	2	1	dep	Gram-staining-positive	69:90	arg1	endospore-forming					93:109	endospore-forming	93:109	endospore-forming	93:109	A Gram-staining-positive, endospore-forming, aerobic strain, designed WN066T, was isolated from saline-alkali wetland soil of Tianjin, China.
33625539	5	2	dep	NaCl	901:904	arg1	w/v					923:925	w/v	923:925	w/v	923:925	The strain grew at 19-42 °C (optimally 33-37 °C) in the presence of 3-20% (w/v) NaCl (optimally 8-12%(w/v)), and at pH 6.5-11.0 (optimally 7.5-8.5).
33625539	5	2	dep	NaCl	901:904	arg1	%					921:921	optimally 8-12%	907:921	optimally 8-12%(w/v)	907:926	The strain grew at 19-42 °C (optimally 33-37 °C) in the presence of 3-20% (w/v) NaCl (optimally 8-12%(w/v)), and at pH 6.5-11.0 (optimally 7.5-8.5).
33625539	12	3	theme	33953T = ACCC	1596:1608	arg1	60085T					1610:1615	= KCTC 33953T = ACCC 60085T	1589:1615	= KCTC 33953T = ACCC 60085T	1589:1615	The type strain is WN066T (= KCTC 33953T = ACCC 60085T).
33625539	12	3	theme	33953T = ACCC	1596:1608	arg1	WN066T					1581:1586	WN066T	1581:1586	WN066T (= KCTC 33953T = ACCC 60085T)	1581:1616	The type strain is WN066T (= KCTC 33953T = ACCC 60085T).
33625539	3	4	theme	DSM	444:446	arg1	15601T					448:453	Bacillus bataviensis DSM 15601T	423:453	Bacillus bataviensis DSM 15601T (98.7%)	423:461	Phylogenetic analysis based on 16S rRNA gene sequence indicated WN066T was a member of the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%), Bacillus cucumis CCM 8651T (98.8%), Bacillus bataviensis DSM 15601T (98.7%) and Bacillus niacini DSM 2923T (98.7%).
33625539	3	4	theme	DSM	444:446	arg1	%					460:460	98.7%	456:460	98.7%	456:460	Phylogenetic analysis based on 16S rRNA gene sequence indicated WN066T was a member of the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%), Bacillus cucumis CCM 8651T (98.8%), Bacillus bataviensis DSM 15601T (98.7%) and Bacillus niacini DSM 2923T (98.7%).
33625539	8	5	theme	genome	1206:1211	arg1	size					1188:1191	The size	1184:1191	The size of the draft genome	1184:1211	The size of the draft genome was 6,213,503 bp in size and had a G + C content of 38.6 mol %.
33625539	8	5	theme	genome	1206:1211	arg1	6,213,503 bp					1217:1228	6,213,503 bp	1217:1228	6,213,503 bp	1217:1228	The size of the draft genome was 6,213,503 bp in size and had a G + C content of 38.6 mol %.
33625539	1	6	theme	saline-alkaline	45:59	arg1	soil					61:64	saline-alkaline soil	45:64	saline-alkaline soil	45:64	nov., isolated from saline-alkaline soil.
33625539	9	7	theme	diamino	1348:1354	arg1	acid					1356:1359	the diagnostic diamino acid	1333:1359	the diagnostic diamino acid	1333:1359	The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
33625539	9	7	theme	diamino	1348:1354	arg1	acid					1325:1328	meso-diaminopimelic acid	1305:1328	meso-diaminopimelic acid	1305:1328	The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
33625539	10	8	theme	novel	1465:1469	arg1	species					1471:1477	a novel species	1463:1477	a novel species	1463:1477	On basis of phenotypic, phylogenetic, chemotaxonomic and genomic features, strain WN066T represented a novel species within the genus Bacillus, for which the name B. salipaludis sp.
33625539	3	9	theme	related	336:342	arg1	15600T					371:376	Bacillus, and most closely related to Bacillus drentensis DSM 15600T	309:376	15600T	371:376	Phylogenetic analysis based on 16S rRNA gene sequence indicated WN066T was a member of the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%), Bacillus cucumis CCM 8651T (98.8%), Bacillus bataviensis DSM 15601T (98.7%) and Bacillus niacini DSM 2923T (98.7%).
33625539	8	10	theme	%	1274:1274	arg1	content					1254:1260	a G + C content	1246:1260	a G + C content of 38.6 mol %	1246:1274	The size of the draft genome was 6,213,503 bp in size and had a G + C content of 38.6 mol %.
33625539	3	11	theme	Phylogenetic	209:220	arg1	analysis					222:229	Phylogenetic analysis	209:229	Phylogenetic analysis based on 16S rRNA gene sequence	209:261	Phylogenetic analysis based on 16S rRNA gene sequence indicated WN066T was a member of the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%), Bacillus cucumis CCM 8651T (98.8%), Bacillus bataviensis DSM 15601T (98.7%) and Bacillus niacini DSM 2923T (98.7%).
33625539	6	12	theme	major	974:978	arg1	iso-C15:0					1006:1014	iso-C15:0	1006:1014	iso-C15:0	1006:1014	The major cellular fatty acids were iso-C15:0 (24.8%) and anteiso-C15:0 (38.9%).
33625539	6	12	theme	major	974:978	arg1	acids					995:999	The major cellular fatty acids	970:999	The major cellular fatty acids	970:999	The major cellular fatty acids were iso-C15:0 (24.8%) and anteiso-C15:0 (38.9%).
33625539	8	13	from	size	1233:1236	arg1	size					1188:1191	The size	1184:1191	The size of the draft genome	1184:1211	The size of the draft genome was 6,213,503 bp in size and had a G + C content of 38.6 mol %.
33625539	8	13	from	size	1233:1236	arg1	6,213,503 bp					1217:1228	6,213,503 bp	1217:1228	6,213,503 bp	1217:1228	The size of the draft genome was 6,213,503 bp in size and had a G + C content of 38.6 mol %.
33625539	8	14	theme	38.6 mol	1265:1272	arg1	%					1274:1274	38.6 mol %	1265:1274	38.6 mol %	1265:1274	The size of the draft genome was 6,213,503 bp in size and had a G + C content of 38.6 mol %.
33625539	3	15	theme	rRNA	244:247	arg1	sequence					254:261	16S rRNA gene sequence	240:261	16S rRNA gene sequence	240:261	Phylogenetic analysis based on 16S rRNA gene sequence indicated WN066T was a member of the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%), Bacillus cucumis CCM 8651T (98.8%), Bacillus bataviensis DSM 15601T (98.7%) and Bacillus niacini DSM 2923T (98.7%).
33625539	10	16	theme	strain	1437:1442	arg1	WN066T					1444:1449	strain WN066T	1437:1449	strain WN066T	1437:1449	On basis of phenotypic, phylogenetic, chemotaxonomic and genomic features, strain WN066T represented a novel species within the genus Bacillus, for which the name B. salipaludis sp.
33625539	9	17	theme	meso-diaminopimelic	1305:1323	arg1	acid					1356:1359	the diagnostic diamino acid	1333:1359	the diagnostic diamino acid	1333:1359	The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
33625539	9	17	theme	meso-diaminopimelic	1305:1323	arg1	acid					1325:1328	meso-diaminopimelic acid	1305:1328	meso-diaminopimelic acid	1305:1328	The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
33625539	10	18	theme	chemotaxonomic	1400:1413	arg1	features					1427:1434	phenotypic, phylogenetic, chemotaxonomic and genomic features	1374:1434	phenotypic, phylogenetic, chemotaxonomic and genomic features	1374:1434	On basis of phenotypic, phylogenetic, chemotaxonomic and genomic features, strain WN066T represented a novel species within the genus Bacillus, for which the name B. salipaludis sp.
33625539	10	19	theme	genomic	1419:1425	arg1	features					1427:1434	phenotypic, phylogenetic, chemotaxonomic and genomic features	1374:1434	phenotypic, phylogenetic, chemotaxonomic and genomic features	1374:1434	On basis of phenotypic, phylogenetic, chemotaxonomic and genomic features, strain WN066T represented a novel species within the genus Bacillus, for which the name B. salipaludis sp.
33625539	3	20	theme	gene	249:252	arg1	sequence					254:261	16S rRNA gene sequence	240:261	16S rRNA gene sequence	240:261	Phylogenetic analysis based on 16S rRNA gene sequence indicated WN066T was a member of the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%), Bacillus cucumis CCM 8651T (98.8%), Bacillus bataviensis DSM 15601T (98.7%) and Bacillus niacini DSM 2923T (98.7%).
33625539	5	21	dep	grew	832:835	arg1	7.5-8.5					960:966	7.5-8.5	960:966	7.5-8.5	960:966	The strain grew at 19-42 °C (optimally 33-37 °C) in the presence of 3-20% (w/v) NaCl (optimally 8-12%(w/v)), and at pH 6.5-11.0 (optimally 7.5-8.5).
33625539	3	22	theme	DSM	367:369	arg1	15600T					371:376	Bacillus, and most closely related to Bacillus drentensis DSM 15600T	309:376	15600T	371:376	Phylogenetic analysis based on 16S rRNA gene sequence indicated WN066T was a member of the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%), Bacillus cucumis CCM 8651T (98.8%), Bacillus bataviensis DSM 15601T (98.7%) and Bacillus niacini DSM 2923T (98.7%).
33625539	3	23	theme	cucumis	396:402	arg1	8651T					408:412	cucumis CCM 8651T	396:412	cucumis CCM 8651T (98.8%)	396:420	Phylogenetic analysis based on 16S rRNA gene sequence indicated WN066T was a member of the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%), Bacillus cucumis CCM 8651T (98.8%), Bacillus bataviensis DSM 15601T (98.7%) and Bacillus niacini DSM 2923T (98.7%).
33625539	3	23	theme	cucumis	396:402	arg1	%					419:419	98.8%	415:419	98.8%	415:419	Phylogenetic analysis based on 16S rRNA gene sequence indicated WN066T was a member of the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%), Bacillus cucumis CCM 8651T (98.8%), Bacillus bataviensis DSM 15601T (98.7%) and Bacillus niacini DSM 2923T (98.7%).
33625539	0	24	theme	salipaludis	9:19	arg1	sp					21:22	Bacillus salipaludis sp	0:22	Bacillus salipaludis sp.	0:23	Bacillus salipaludis sp.
33625539	7	25	theme	predominant	1055:1065	arg1	lipids					1073:1078	The predominant polar lipids	1051:1078	The predominant polar lipids	1051:1078	The predominant polar lipids consisted of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and phosphatidylethanolamine (PE).
33625539	8	26	from	6,213,503 bp	1217:1228	arg1	size					1233:1236	size	1233:1236	size	1233:1236	The size of the draft genome was 6,213,503 bp in size and had a G + C content of 38.6 mol %.
33625539	3	27	theme	DSM	484:486	arg1	%					499:499	98.7%	495:499	98.7%	495:499	Phylogenetic analysis based on 16S rRNA gene sequence indicated WN066T was a member of the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%), Bacillus cucumis CCM 8651T (98.8%), Bacillus bataviensis DSM 15601T (98.7%) and Bacillus niacini DSM 2923T (98.7%).
33625539	3	27	theme	DSM	484:486	arg1	2923T					488:492	Bacillus niacini DSM 2923T	467:492	Bacillus niacini DSM 2923T (98.7%)	467:500	Phylogenetic analysis based on 16S rRNA gene sequence indicated WN066T was a member of the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%), Bacillus cucumis CCM 8651T (98.8%), Bacillus bataviensis DSM 15601T (98.7%) and Bacillus niacini DSM 2923T (98.7%).
33625539	4	28	theme	digital	554:560	arg1	DDH					585:587	DDH	585:587	DDH	585:587	However, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) values between strain WN066T and the most closely related species were less than the previously proposed cutoff values for differentiating species within the genus, suggesting that this strain represented a novel Bacillus species.
33625539	4	28	theme	digital	554:560	arg1	hybridization					570:582	digital DNA-DNA hybridization	554:582	digital DNA-DNA hybridization (DDH)	554:588	However, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) values between strain WN066T and the most closely related species were less than the previously proposed cutoff values for differentiating species within the genus, suggesting that this strain represented a novel Bacillus species.
33625539	0	29	theme	Bacillus	0:7	arg1	sp					21:22	Bacillus salipaludis sp	0:22	Bacillus salipaludis sp.	0:23	Bacillus salipaludis sp.
33625539	3	30	theme	16S	240:242	arg1	rRNA					244:247	16S rRNA	240:247	16S rRNA gene sequence	240:261	Phylogenetic analysis based on 16S rRNA gene sequence indicated WN066T was a member of the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%), Bacillus cucumis CCM 8651T (98.8%), Bacillus bataviensis DSM 15601T (98.7%) and Bacillus niacini DSM 2923T (98.7%).
33625539	5	31	theme	w/v	896:898	arg1	NaCl					901:904	3-20% (w/v) NaCl	889:904	3-20% (w/v) NaCl (optimally 8-12%(w/v))	889:927	The strain grew at 19-42 °C (optimally 33-37 °C) in the presence of 3-20% (w/v) NaCl (optimally 8-12%(w/v)), and at pH 6.5-11.0 (optimally 7.5-8.5).
33625539	4	32	theme	related	640:646	arg1	species					648:654	the most closely related species	623:654	the most closely related species	623:654	However, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) values between strain WN066T and the most closely related species were less than the previously proposed cutoff values for differentiating species within the genus, suggesting that this strain represented a novel Bacillus species.
33625539	8	33	theme	draft	1200:1204	arg1	genome					1206:1211	the draft genome	1196:1211	the draft genome	1196:1211	The size of the draft genome was 6,213,503 bp in size and had a G + C content of 38.6 mol %.
33625539	4	34	theme	Bacillus	803:810	arg1	species					812:818	a novel Bacillus species	795:818	a novel Bacillus species	795:818	However, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) values between strain WN066T and the most closely related species were less than the previously proposed cutoff values for differentiating species within the genus, suggesting that this strain represented a novel Bacillus species.
33625539	3	35	theme	Bacillus	467:474	arg1	%					499:499	98.7%	495:499	98.7%	495:499	Phylogenetic analysis based on 16S rRNA gene sequence indicated WN066T was a member of the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%), Bacillus cucumis CCM 8651T (98.8%), Bacillus bataviensis DSM 15601T (98.7%) and Bacillus niacini DSM 2923T (98.7%).
33625539	3	35	theme	Bacillus	467:474	arg1	2923T					488:492	Bacillus niacini DSM 2923T	467:492	Bacillus niacini DSM 2923T (98.7%)	467:500	Phylogenetic analysis based on 16S rRNA gene sequence indicated WN066T was a member of the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%), Bacillus cucumis CCM 8651T (98.8%), Bacillus bataviensis DSM 15601T (98.7%) and Bacillus niacini DSM 2923T (98.7%).
33625539	4	36	theme	nucleotide	524:533	arg1	identity					535:542	average nucleotide identity	516:542	average nucleotide identity (ANI)	516:548	However, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) values between strain WN066T and the most closely related species were less than the previously proposed cutoff values for differentiating species within the genus, suggesting that this strain represented a novel Bacillus species.
33625539	4	36	theme	nucleotide	524:533	arg1	ANI					545:547	ANI	545:547	ANI	545:547	However, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) values between strain WN066T and the most closely related species were less than the previously proposed cutoff values for differentiating species within the genus, suggesting that this strain represented a novel Bacillus species.
33625539	4	37	theme	novel	797:801	arg1	species					812:818	a novel Bacillus species	795:818	a novel Bacillus species	795:818	However, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) values between strain WN066T and the most closely related species were less than the previously proposed cutoff values for differentiating species within the genus, suggesting that this strain represented a novel Bacillus species.
33625539	6	38	theme	cellular	980:987	arg1	iso-C15:0					1006:1014	iso-C15:0	1006:1014	iso-C15:0	1006:1014	The major cellular fatty acids were iso-C15:0 (24.8%) and anteiso-C15:0 (38.9%).
33625539	6	38	theme	cellular	980:987	arg1	acids					995:999	The major cellular fatty acids	970:999	The major cellular fatty acids	970:999	The major cellular fatty acids were iso-C15:0 (24.8%) and anteiso-C15:0 (38.9%).
33625539	3	39	theme	niacini	476:482	arg1	%					499:499	98.7%	495:499	98.7%	495:499	Phylogenetic analysis based on 16S rRNA gene sequence indicated WN066T was a member of the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%), Bacillus cucumis CCM 8651T (98.8%), Bacillus bataviensis DSM 15601T (98.7%) and Bacillus niacini DSM 2923T (98.7%).
33625539	3	39	theme	niacini	476:482	arg1	2923T					488:492	Bacillus niacini DSM 2923T	467:492	Bacillus niacini DSM 2923T (98.7%)	467:500	Phylogenetic analysis based on 16S rRNA gene sequence indicated WN066T was a member of the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%), Bacillus cucumis CCM 8651T (98.8%), Bacillus bataviensis DSM 15601T (98.7%) and Bacillus niacini DSM 2923T (98.7%).
33625539	4	40	theme	average	516:522	arg1	identity					535:542	average nucleotide identity	516:542	average nucleotide identity (ANI)	516:548	However, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) values between strain WN066T and the most closely related species were less than the previously proposed cutoff values for differentiating species within the genus, suggesting that this strain represented a novel Bacillus species.
33625539	4	40	theme	average	516:522	arg1	ANI					545:547	ANI	545:547	ANI	545:547	However, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) values between strain WN066T and the most closely related species were less than the previously proposed cutoff values for differentiating species within the genus, suggesting that this strain represented a novel Bacillus species.
33625539	10	41	theme	phylogenetic	1386:1397	arg1	features					1427:1434	phenotypic, phylogenetic, chemotaxonomic and genomic features	1374:1434	phenotypic, phylogenetic, chemotaxonomic and genomic features	1374:1434	On basis of phenotypic, phylogenetic, chemotaxonomic and genomic features, strain WN066T represented a novel species within the genus Bacillus, for which the name B. salipaludis sp.
33625539	3	42	theme	Bacillus	423:430	arg1	15601T					448:453	Bacillus bataviensis DSM 15601T	423:453	Bacillus bataviensis DSM 15601T (98.7%)	423:461	Phylogenetic analysis based on 16S rRNA gene sequence indicated WN066T was a member of the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%), Bacillus cucumis CCM 8651T (98.8%), Bacillus bataviensis DSM 15601T (98.7%) and Bacillus niacini DSM 2923T (98.7%).
33625539	3	42	theme	Bacillus	423:430	arg1	%					460:460	98.7%	456:460	98.7%	456:460	Phylogenetic analysis based on 16S rRNA gene sequence indicated WN066T was a member of the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%), Bacillus cucumis CCM 8651T (98.8%), Bacillus bataviensis DSM 15601T (98.7%) and Bacillus niacini DSM 2923T (98.7%).
33625539	9	43	theme	diagnostic	1337:1346	arg1	acid					1356:1359	the diagnostic diamino acid	1333:1359	the diagnostic diamino acid	1333:1359	The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
33625539	9	43	theme	diagnostic	1337:1346	arg1	acid					1325:1328	meso-diaminopimelic acid	1305:1328	meso-diaminopimelic acid	1305:1328	The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
33625539	2	44	theme	wetland	177:183	arg1	soil					185:188	saline-alkali wetland soil	163:188	saline-alkali wetland soil of Tianjin, China	163:206	A Gram-staining-positive, endospore-forming, aerobic strain, designed WN066T, was isolated from saline-alkali wetland soil of Tianjin, China.
33625539	4	45	theme	cutoff	695:700	arg1	values					702:707	the previously proposed cutoff values	671:707	the previously proposed cutoff values for differentiating species within the genus	671:752	However, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) values between strain WN066T and the most closely related species were less than the previously proposed cutoff values for differentiating species within the genus, suggesting that this strain represented a novel Bacillus species.
33625539	10	46	dep	genus	1490:1494	arg1	Bacillus					1496:1503	the genus Bacillus	1486:1503	the genus Bacillus	1486:1503	On basis of phenotypic, phylogenetic, chemotaxonomic and genomic features, strain WN066T represented a novel species within the genus Bacillus, for which the name B. salipaludis sp.
33625539	3	47	theme	CCM	404:406	arg1	8651T					408:412	cucumis CCM 8651T	396:412	cucumis CCM 8651T (98.8%)	396:420	Phylogenetic analysis based on 16S rRNA gene sequence indicated WN066T was a member of the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%), Bacillus cucumis CCM 8651T (98.8%), Bacillus bataviensis DSM 15601T (98.7%) and Bacillus niacini DSM 2923T (98.7%).
33625539	3	47	theme	CCM	404:406	arg1	%					419:419	98.8%	415:419	98.8%	415:419	Phylogenetic analysis based on 16S rRNA gene sequence indicated WN066T was a member of the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%), Bacillus cucumis CCM 8651T (98.8%), Bacillus bataviensis DSM 15601T (98.7%) and Bacillus niacini DSM 2923T (98.7%).
33625539	2	48	theme	saline-alkali	163:175	arg1	soil					185:188	saline-alkali wetland soil	163:188	saline-alkali wetland soil of Tianjin, China	163:206	A Gram-staining-positive, endospore-forming, aerobic strain, designed WN066T, was isolated from saline-alkali wetland soil of Tianjin, China.
33625539	4	49	theme	proposed	686:693	arg1	values					702:707	the previously proposed cutoff values	671:707	the previously proposed cutoff values for differentiating species within the genus	671:752	However, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) values between strain WN066T and the most closely related species were less than the previously proposed cutoff values for differentiating species within the genus, suggesting that this strain represented a novel Bacillus species.
33625539	4	50	theme	identity	535:542	arg1	values					590:595	the average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) values	512:595	the average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) values between strain WN066T and the most closely related species	512:654	However, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) values between strain WN066T and the most closely related species were less than the previously proposed cutoff values for differentiating species within the genus, suggesting that this strain represented a novel Bacillus species.
33625539	4	50	theme	identity	535:542	arg1	less					661:664	less	661:664	less	661:664	However, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) values between strain WN066T and the most closely related species were less than the previously proposed cutoff values for differentiating species within the genus, suggesting that this strain represented a novel Bacillus species.
33625539	3	51	theme	15600T	371:376	arg1	genus					300:304	the genus	296:304	the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%)	296:384	Phylogenetic analysis based on 16S rRNA gene sequence indicated WN066T was a member of the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%), Bacillus cucumis CCM 8651T (98.8%), Bacillus bataviensis DSM 15601T (98.7%) and Bacillus niacini DSM 2923T (98.7%).
33625539	5	52	theme	%	893:893	arg1	NaCl					901:904	3-20% (w/v) NaCl	889:904	3-20% (w/v) NaCl (optimally 8-12%(w/v))	889:927	The strain grew at 19-42 °C (optimally 33-37 °C) in the presence of 3-20% (w/v) NaCl (optimally 8-12%(w/v)), and at pH 6.5-11.0 (optimally 7.5-8.5).
33625539	12	53	theme	= KCTC	1589:1594	arg1	60085T					1610:1615	= KCTC 33953T = ACCC 60085T	1589:1615	= KCTC 33953T = ACCC 60085T	1589:1615	The type strain is WN066T (= KCTC 33953T = ACCC 60085T).
33625539	12	53	theme	= KCTC	1589:1594	arg1	WN066T					1581:1586	WN066T	1581:1586	WN066T (= KCTC 33953T = ACCC 60085T)	1581:1616	The type strain is WN066T (= KCTC 33953T = ACCC 60085T).
33625539	10	54	dep	B.	1525:1526	arg1	salipaludis					1528:1538	salipaludis	1528:1538	salipaludis	1528:1538	On basis of phenotypic, phylogenetic, chemotaxonomic and genomic features, strain WN066T represented a novel species within the genus Bacillus, for which the name B. salipaludis sp.
33625539	2	55	theme	China	202:206	arg1	soil					185:188	saline-alkali wetland soil	163:188	saline-alkali wetland soil of Tianjin, China	163:206	A Gram-staining-positive, endospore-forming, aerobic strain, designed WN066T, was isolated from saline-alkali wetland soil of Tianjin, China.
33625539	4	56	theme	differentiating	713:727	arg1	species					729:735	differentiating species	713:735	differentiating species within the genus	713:752	However, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) values between strain WN066T and the most closely related species were less than the previously proposed cutoff values for differentiating species within the genus, suggesting that this strain represented a novel Bacillus species.
33625539	2	57	attach	isolated	149:156	arg1	soil					185:188	saline-alkali wetland soil	163:188	saline-alkali wetland soil of Tianjin, China	163:206	A Gram-staining-positive, endospore-forming, aerobic strain, designed WN066T, was isolated from saline-alkali wetland soil of Tianjin, China.
33625539	2	57	attach	isolated	149:156	arg2	strain					120:125	A Gram-staining-positive, endospore-forming, aerobic strain	67:125	A Gram-staining-positive, endospore-forming, aerobic strain	67:125	A Gram-staining-positive, endospore-forming, aerobic strain, designed WN066T, was isolated from saline-alkali wetland soil of Tianjin, China.
33625539	10	58	theme	name	1520:1523	arg1	sp					1540:1541	the name B. salipaludis sp	1516:1541	the name B. salipaludis sp	1516:1541	On basis of phenotypic, phylogenetic, chemotaxonomic and genomic features, strain WN066T represented a novel species within the genus Bacillus, for which the name B. salipaludis sp.
33625539	2	59	theme	Gram-staining-positive	69:90	arg1	strain					120:125	A Gram-staining-positive, endospore-forming, aerobic strain	67:125	A Gram-staining-positive, endospore-forming, aerobic strain	67:125	A Gram-staining-positive, endospore-forming, aerobic strain, designed WN066T, was isolated from saline-alkali wetland soil of Tianjin, China.
33625539	9	60	contain	contained	1295:1303	arg2	acid					1356:1359	the diagnostic diamino acid	1333:1359	the diagnostic diamino acid	1333:1359	The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
33625539	9	60	contain	contained	1295:1303	arg1	peptidoglycan					1281:1293	The peptidoglycan	1277:1293	The peptidoglycan	1277:1293	The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
33625539	9	60	contain	contained	1295:1303	arg2	acid					1325:1328	meso-diaminopimelic acid	1305:1328	meso-diaminopimelic acid	1305:1328	The peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid.
33625539	12	61	theme	type	1566:1569	arg1	WN066T					1581:1586	WN066T	1581:1586	WN066T (= KCTC 33953T = ACCC 60085T)	1581:1616	The type strain is WN066T (= KCTC 33953T = ACCC 60085T).
33625539	12	61	theme	type	1566:1569	arg1	strain					1571:1576	The type strain	1562:1576	The type strain	1562:1576	The type strain is WN066T (= KCTC 33953T = ACCC 60085T).
33625539	4	62	theme	strain	605:610	arg1	WN066T					612:617	strain WN066T	605:617	strain WN066T	605:617	However, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) values between strain WN066T and the most closely related species were less than the previously proposed cutoff values for differentiating species within the genus, suggesting that this strain represented a novel Bacillus species.
33625539	8	63	contain	had	1242:1244	arg1	size					1188:1191	The size	1184:1191	The size of the draft genome	1184:1211	The size of the draft genome was 6,213,503 bp in size and had a G + C content of 38.6 mol %.
33625539	8	63	contain	had	1242:1244	arg1	6,213,503 bp					1217:1228	6,213,503 bp	1217:1228	6,213,503 bp	1217:1228	The size of the draft genome was 6,213,503 bp in size and had a G + C content of 38.6 mol %.
33625539	8	63	contain	had	1242:1244	arg2	content					1254:1260	a G + C content	1246:1260	a G + C content of 38.6 mol %	1246:1274	The size of the draft genome was 6,213,503 bp in size and had a G + C content of 38.6 mol %.
33625539	3	64	dep	Bacillus	423:430	arg1	bataviensis					432:442	bataviensis	432:442	bataviensis	432:442	Phylogenetic analysis based on 16S rRNA gene sequence indicated WN066T was a member of the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%), Bacillus cucumis CCM 8651T (98.8%), Bacillus bataviensis DSM 15601T (98.7%) and Bacillus niacini DSM 2923T (98.7%).
33625539	5	65	theme	NaCl	901:904	arg1	presence					877:884	the presence	873:884	the presence of 3-20% (w/v) NaCl (optimally 8-12%(w/v))	873:927	The strain grew at 19-42 °C (optimally 33-37 °C) in the presence of 3-20% (w/v) NaCl (optimally 8-12%(w/v)), and at pH 6.5-11.0 (optimally 7.5-8.5).
33625539	3	66	dep	8651T	408:412	arg1	Bacillus					387:394	Bacillus	387:394	Bacillus	387:394	Phylogenetic analysis based on 16S rRNA gene sequence indicated WN066T was a member of the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%), Bacillus cucumis CCM 8651T (98.8%), Bacillus bataviensis DSM 15601T (98.7%) and Bacillus niacini DSM 2923T (98.7%).
33625539	10	67	theme	features	1427:1434	arg1	basis					1365:1369	basis	1365:1369	basis of phenotypic, phylogenetic, chemotaxonomic and genomic features	1365:1434	On basis of phenotypic, phylogenetic, chemotaxonomic and genomic features, strain WN066T represented a novel species within the genus Bacillus, for which the name B. salipaludis sp.
33625539	10	68	theme	B.	1525:1526	arg1	sp					1540:1541	the name B. salipaludis sp	1516:1541	the name B. salipaludis sp	1516:1541	On basis of phenotypic, phylogenetic, chemotaxonomic and genomic features, strain WN066T represented a novel species within the genus Bacillus, for which the name B. salipaludis sp.
33625539	3	69	theme	Bacillus	309:316	arg1	genus					300:304	the genus	296:304	the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%)	296:384	Phylogenetic analysis based on 16S rRNA gene sequence indicated WN066T was a member of the genus of Bacillus, and most closely related to Bacillus drentensis DSM 15600T (98.9%), Bacillus cucumis CCM 8651T (98.8%), Bacillus bataviensis DSM 15601T (98.7%) and Bacillus niacini DSM 2923T (98.7%).
33625539	7	70	theme	polar	1067:1071	arg1	lipids					1073:1078	The predominant polar lipids	1051:1078	The predominant polar lipids	1051:1078	The predominant polar lipids consisted of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and phosphatidylethanolamine (PE).
33625539	4	71	theme	hybridization	570:582	arg1	values					590:595	the average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) values	512:595	the average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) values between strain WN066T and the most closely related species	512:654	However, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) values between strain WN066T and the most closely related species were less than the previously proposed cutoff values for differentiating species within the genus, suggesting that this strain represented a novel Bacillus species.
33625539	4	71	theme	hybridization	570:582	arg1	less					661:664	less	661:664	less	661:664	However, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) values between strain WN066T and the most closely related species were less than the previously proposed cutoff values for differentiating species within the genus, suggesting that this strain represented a novel Bacillus species.
33625539	10	72	theme	phenotypic	1374:1383	arg1	features					1427:1434	phenotypic, phylogenetic, chemotaxonomic and genomic features	1374:1434	phenotypic, phylogenetic, chemotaxonomic and genomic features	1374:1434	On basis of phenotypic, phylogenetic, chemotaxonomic and genomic features, strain WN066T represented a novel species within the genus Bacillus, for which the name B. salipaludis sp.
33625539	8	73	theme	G + C	1248:1252	arg1	content					1254:1260	a G + C content	1246:1260	a G + C content of 38.6 mol %	1246:1274	The size of the draft genome was 6,213,503 bp in size and had a G + C content of 38.6 mol %.
33625539	6	74	theme	fatty	989:993	arg1	iso-C15:0					1006:1014	iso-C15:0	1006:1014	iso-C15:0	1006:1014	The major cellular fatty acids were iso-C15:0 (24.8%) and anteiso-C15:0 (38.9%).
33625539	6	74	theme	fatty	989:993	arg1	acids					995:999	The major cellular fatty acids	970:999	The major cellular fatty acids	970:999	The major cellular fatty acids were iso-C15:0 (24.8%) and anteiso-C15:0 (38.9%).
33625539	4	75	theme	DNA-DNA	562:568	arg1	DDH					585:587	DDH	585:587	DDH	585:587	However, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) values between strain WN066T and the most closely related species were less than the previously proposed cutoff values for differentiating species within the genus, suggesting that this strain represented a novel Bacillus species.
33625539	4	75	theme	DNA-DNA	562:568	arg1	hybridization					570:582	digital DNA-DNA hybridization	554:582	digital DNA-DNA hybridization (DDH)	554:588	However, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) values between strain WN066T and the most closely related species were less than the previously proposed cutoff values for differentiating species within the genus, suggesting that this strain represented a novel Bacillus species.
32993494	10	0	theme	biomass	1698:1704	arg1	group					1706:1710	the high biomass group	1689:1710	the high biomass group	1689:1710	Genes in pathways related to biosynthesis of cell wall components and expansins coding genes showed low average expression levels and were mostly upregulated in the high biomass group.
32993494	9	1	theme	sucrose	1412:1418	arg1	synthesis					1420:1428	sucrose synthesis	1412:1428	sucrose synthesis in leaves	1412:1438	Sucrose synthase (SuSy) coding genes were upregulated in the low biomass group, showing that this enzyme class can be involved with sucrose synthesis in leaves, similarly to sucrose phosphate synthase (SPS) and sucrose phosphate phosphatase (SPP).
32993494	12	2	theme	contrasting	2021:2031	arg1	genotypes					2033:2041	contrasting genotypes	2021:2041	contrasting genotypes	2021:2041	Our data from sugarcane leaves revealed how hybridization in a complex polyploid system resulted in noticeably different transcriptomic profiles between contrasting genotypes.
32993494	4	3	theme	First	601:605	arg1	dewlap					615:620	First visible dewlap leaves	601:627	First visible dewlap leaves	601:627	RESULTS First visible dewlap leaves were collected from 12 genotypes, six per group, to perform RNA-Seq.
32993494	11	4	dep	CONCLUSIONS	1713:1723	arg1	show					1749:1752	show	1749:1752	show differences in carbohydrate synthesis and carbon partitioning in the source tissue of distinct phenotypic groups	1749:1865	CONCLUSIONS Together, these results show differences in carbohydrate synthesis and carbon partitioning in the source tissue of distinct phenotypic groups.
32993494	2	5	theme	fiber	389:393	arg1	content					395:401	fiber content	389:401	fiber content	389:401	Achieving these goals in an energy crop like sugarcane relies on breeding for sucrose accumulation, fiber content and stalk number.
32993494	8	6	theme	high	1260:1263	arg1	group					1273:1277	the high biomass group	1256:1277	the high biomass group	1256:1277	However, most genes coding for photosystem components or those coding for phosphoenolpyruvate carboxylases (PEPCs) were upregulated in the high biomass group.
32993494	6	7	from	differences	889:899	arg1	defense					922:928	defense	922:928	defense	922:928	We found evidence that differences in transposition and defense related genes may arise due to the complex nature of the polyploid Saccharum genomes.
32993494	6	7	from	differences	889:899	arg1	transposition					904:916	transposition	904:916	transposition	904:916	We found evidence that differences in transposition and defense related genes may arise due to the complex nature of the polyploid Saccharum genomes.
32993494	0	8	theme	Differential	0:11	arg1	expression					13:22	Differential expression	0:22	Differential expression in leaves of Saccharum genotypes contrasting in biomass production	0:89	Differential expression in leaves of Saccharum genotypes contrasting in biomass production provides evidence of genes involved in carbon partitioning.
32993494	1	9	theme	crops	189:193	arg1	development					166:176	The development	162:176	The development of biomass crops	162:193	BACKGROUND The development of biomass crops aims to meet industrial yield demands, in order to optimize profitability and sustainability.
32993494	12	10	theme	different	1979:1987	arg1	profiles					2004:2011	noticeably different transcriptomic profiles	1968:2011	noticeably different transcriptomic profiles between contrasting genotypes	1968:2041	Our data from sugarcane leaves revealed how hybridization in a complex polyploid system resulted in noticeably different transcriptomic profiles between contrasting genotypes.
32993494	10	11	theme	wall	1578:1581	arg1	components					1583:1592	cell wall components	1573:1592	cell wall components	1573:1592	Genes in pathways related to biosynthesis of cell wall components and expansins coding genes showed low average expression levels and were mostly upregulated in the high biomass group.
32993494	11	12	theme	carbon	1796:1801	arg1	partitioning					1803:1814	carbon partitioning	1796:1814	carbon partitioning	1796:1814	CONCLUSIONS Together, these results show differences in carbohydrate synthesis and carbon partitioning in the source tissue of distinct phenotypic groups.
32993494	2	13	theme	energy	317:322	arg1	crop					324:327	an energy crop	314:327	an energy crop like sugarcane	314:342	Achieving these goals in an energy crop like sugarcane relies on breeding for sucrose accumulation, fiber content and stalk number.
32993494	6	14	theme	complex	965:971	arg1	nature					973:978	the complex nature	961:978	the complex nature of the polyploid Saccharum genomes	961:1013	We found evidence that differences in transposition and defense related genes may arise due to the complex nature of the polyploid Saccharum genomes.
32993494	12	15	dep	sugarcane	1882:1890	arg1	leaves					1892:1897	leaves	1892:1897	leaves	1892:1897	Our data from sugarcane leaves revealed how hybridization in a complex polyploid system resulted in noticeably different transcriptomic profiles between contrasting genotypes.
32993494	1	16	theme	industrial	208:217	arg1	demands					225:231	industrial yield demands	208:231	industrial yield demands	208:231	BACKGROUND The development of biomass crops aims to meet industrial yield demands, in order to optimize profitability and sustainability.
32993494	10	17	theme	coding	1608:1613	arg1	genes					1615:1619	expansins coding genes	1598:1619	expansins coding genes	1598:1619	Genes in pathways related to biosynthesis of cell wall components and expansins coding genes showed low average expression levels and were mostly upregulated in the high biomass group.
32993494	11	18	theme	source	1823:1828	arg1	tissue					1830:1835	the source tissue	1819:1835	the source tissue of distinct phenotypic groups	1819:1865	CONCLUSIONS Together, these results show differences in carbohydrate synthesis and carbon partitioning in the source tissue of distinct phenotypic groups.
32993494	7	19	from	variability	1072:1082	arg1	genes					1087:1091	genes	1087:1091	genes involved in photosynthesis	1087:1118	Genotypes within both biomass groups showed substantial variability in genes involved in photosynthesis.
32993494	7	20	theme	biomass	1038:1044	arg1	groups					1046:1051	both biomass groups	1033:1051	both biomass groups	1033:1051	Genotypes within both biomass groups showed substantial variability in genes involved in photosynthesis.
32993494	9	21	theme	synthase	1288:1295	arg1	genes					1311:1315	Sucrose synthase (SuSy) coding genes	1280:1315	Sucrose synthase (SuSy) coding genes	1280:1315	Sucrose synthase (SuSy) coding genes were upregulated in the low biomass group, showing that this enzyme class can be involved with sucrose synthesis in leaves, similarly to sucrose phosphate synthase (SPS) and sucrose phosphate phosphatase (SPP).
32993494	9	22	theme	sucrose	1491:1497	arg1	phosphatase					1509:1519	sucrose phosphate phosphatase	1491:1519	sucrose phosphate phosphatase (SPP)	1491:1525	Sucrose synthase (SuSy) coding genes were upregulated in the low biomass group, showing that this enzyme class can be involved with sucrose synthesis in leaves, similarly to sucrose phosphate synthase (SPS) and sucrose phosphate phosphatase (SPP).
32993494	9	22	theme	sucrose	1491:1497	arg1	SPP					1522:1524	SPP	1522:1524	SPP	1522:1524	Sucrose synthase (SuSy) coding genes were upregulated in the low biomass group, showing that this enzyme class can be involved with sucrose synthesis in leaves, similarly to sucrose phosphate synthase (SPS) and sucrose phosphate phosphatase (SPP).
32993494	5	23	theme	genes	749:753	arg1	number					714:719	a high number	707:719	a high number of differentially expressed genes	707:753	We found a high number of differentially expressed genes, showing how hybridization in a complex polyploid system caused extensive modifications in genome functioning.
32993494	6	24	theme	genomes	1007:1013	arg1	nature					973:978	the complex nature	961:978	the complex nature of the polyploid Saccharum genomes	961:1013	We found evidence that differences in transposition and defense related genes may arise due to the complex nature of the polyploid Saccharum genomes.
32993494	9	25	theme	coding	1304:1309	arg1	genes					1311:1315	Sucrose synthase (SuSy) coding genes	1280:1315	Sucrose synthase (SuSy) coding genes	1280:1315	Sucrose synthase (SuSy) coding genes were upregulated in the low biomass group, showing that this enzyme class can be involved with sucrose synthesis in leaves, similarly to sucrose phosphate synthase (SPS) and sucrose phosphate phosphatase (SPP).
32993494	4	26	dep	dewlap	615:620	arg1	leaves					622:627	leaves	622:627	leaves	622:627	RESULTS First visible dewlap leaves were collected from 12 genotypes, six per group, to perform RNA-Seq.
32993494	6	27	theme	polyploid	987:995	arg1	genomes					1007:1013	the polyploid Saccharum genomes	983:1013	the polyploid Saccharum genomes	983:1013	We found evidence that differences in transposition and defense related genes may arise due to the complex nature of the polyploid Saccharum genomes.
32993494	12	28	from	sugarcane	1882:1890	arg1	data					1872:1875	Our data	1868:1875	Our data from sugarcane leaves	1868:1897	Our data from sugarcane leaves revealed how hybridization in a complex polyploid system resulted in noticeably different transcriptomic profiles between contrasting genotypes.
32993494	10	29	theme	expression	1640:1649	arg1	levels					1651:1656	low average expression levels	1628:1656	low average expression levels	1628:1656	Genes in pathways related to biosynthesis of cell wall components and expansins coding genes showed low average expression levels and were mostly upregulated in the high biomass group.
32993494	0	30	theme	genes	112:116	arg1	evidence					100:107	evidence	100:107	evidence of genes involved in carbon partitioning	100:148	Differential expression in leaves of Saccharum genotypes contrasting in biomass production provides evidence of genes involved in carbon partitioning.
32993494	0	31	theme	biomass	72:78	arg1	production					80:89	biomass production	72:89	biomass production	72:89	Differential expression in leaves of Saccharum genotypes contrasting in biomass production provides evidence of genes involved in carbon partitioning.
32993494	10	32	theme	low	1628:1630	arg1	levels					1651:1656	low average expression levels	1628:1656	low average expression levels	1628:1656	Genes in pathways related to biosynthesis of cell wall components and expansins coding genes showed low average expression levels and were mostly upregulated in the high biomass group.
32993494	5	33	theme	polyploid	795:803	arg1	system					805:810	a complex polyploid system	785:810	a complex polyploid system	785:810	We found a high number of differentially expressed genes, showing how hybridization in a complex polyploid system caused extensive modifications in genome functioning.
32993494	9	34	theme	low	1341:1343	arg1	group					1353:1357	the low biomass group	1337:1357	the low biomass group	1337:1357	Sucrose synthase (SuSy) coding genes were upregulated in the low biomass group, showing that this enzyme class can be involved with sucrose synthesis in leaves, similarly to sucrose phosphate synthase (SPS) and sucrose phosphate phosphatase (SPP).
32993494	1	35	dep	BACKGROUND	151:160	arg1	aims					195:198	aims	195:198	aims	195:198	BACKGROUND The development of biomass crops aims to meet industrial yield demands, in order to optimize profitability and sustainability.
32993494	3	36	theme	pathways	467:474	arg1	understanding					435:447	the understanding	431:447	the understanding of the biological pathways related to these traits	431:498	To expand the understanding of the biological pathways related to these traits, we evaluated gene expression of two groups of genotypes contrasting in biomass composition.
32993494	11	37	theme	phenotypic	1849:1858	arg1	groups					1860:1865	distinct phenotypic groups	1840:1865	distinct phenotypic groups	1840:1865	CONCLUSIONS Together, these results show differences in carbohydrate synthesis and carbon partitioning in the source tissue of distinct phenotypic groups.
32993494	8	38	theme	most	1130:1133	arg1	genes					1135:1139	most genes	1130:1139	most genes coding for photosystem components or those coding for phosphoenolpyruvate carboxylases (PEPCs)	1130:1234	However, most genes coding for photosystem components or those coding for phosphoenolpyruvate carboxylases (PEPCs) were upregulated in the high biomass group.
32993494	4	39	theme	visible	607:613	arg1	dewlap					615:620	First visible dewlap leaves	601:627	First visible dewlap leaves	601:627	RESULTS First visible dewlap leaves were collected from 12 genotypes, six per group, to perform RNA-Seq.
32993494	2	40	theme	stalk	407:411	arg1	number					413:418	stalk number	407:418	stalk number	407:418	Achieving these goals in an energy crop like sugarcane relies on breeding for sucrose accumulation, fiber content and stalk number.
32993494	3	41	theme	gene	514:517	arg1	expression					519:528	gene expression	514:528	gene expression of two groups of genotypes contrasting in biomass composition	514:590	To expand the understanding of the biological pathways related to these traits, we evaluated gene expression of two groups of genotypes contrasting in biomass composition.
32993494	6	42	theme	related	930:936	arg1	genes					938:942	related genes	930:942	related genes	930:942	We found evidence that differences in transposition and defense related genes may arise due to the complex nature of the polyploid Saccharum genomes.
32993494	12	43	from	hybridization	1912:1924	arg1	system					1949:1954	a complex polyploid system	1929:1954	a complex polyploid system	1929:1954	Our data from sugarcane leaves revealed how hybridization in a complex polyploid system resulted in noticeably different transcriptomic profiles between contrasting genotypes.
32993494	8	44	theme	photosystem	1152:1162	arg1	components					1164:1173	photosystem components	1152:1173	photosystem components	1152:1173	However, most genes coding for photosystem components or those coding for phosphoenolpyruvate carboxylases (PEPCs) were upregulated in the high biomass group.
32993494	6	45	dep	transposition	904:916	arg1	genes					938:942	related genes	930:942	related genes	930:942	We found evidence that differences in transposition and defense related genes may arise due to the complex nature of the polyploid Saccharum genomes.
32993494	3	46	theme	groups	537:542	arg1	expression					519:528	gene expression	514:528	gene expression of two groups of genotypes contrasting in biomass composition	514:590	To expand the understanding of the biological pathways related to these traits, we evaluated gene expression of two groups of genotypes contrasting in biomass composition.
32993494	10	47	theme	related	1546:1552	arg1	pathways					1537:1544	pathways	1537:1544	pathways related to biosynthesis of cell wall components	1537:1592	Genes in pathways related to biosynthesis of cell wall components and expansins coding genes showed low average expression levels and were mostly upregulated in the high biomass group.
32993494	10	48	theme	high	1693:1696	arg1	group					1706:1710	the high biomass group	1689:1710	the high biomass group	1689:1710	Genes in pathways related to biosynthesis of cell wall components and expansins coding genes showed low average expression levels and were mostly upregulated in the high biomass group.
32993494	1	49	theme	biomass	181:187	arg1	crops					189:193	biomass crops	181:193	biomass crops	181:193	BACKGROUND The development of biomass crops aims to meet industrial yield demands, in order to optimize profitability and sustainability.
32993494	10	50	from	genes	1615:1619	arg1	pathways					1537:1544	pathways	1537:1544	pathways related to biosynthesis of cell wall components	1537:1592	Genes in pathways related to biosynthesis of cell wall components and expansins coding genes showed low average expression levels and were mostly upregulated in the high biomass group.
32993494	10	51	theme	components	1583:1592	arg1	biosynthesis					1557:1568	biosynthesis	1557:1568	biosynthesis of cell wall components	1557:1592	Genes in pathways related to biosynthesis of cell wall components and expansins coding genes showed low average expression levels and were mostly upregulated in the high biomass group.
32993494	8	52	theme	phosphoenolpyruvate	1195:1213	arg1	PEPCs					1229:1233	PEPCs	1229:1233	PEPCs	1229:1233	However, most genes coding for photosystem components or those coding for phosphoenolpyruvate carboxylases (PEPCs) were upregulated in the high biomass group.
32993494	8	52	theme	phosphoenolpyruvate	1195:1213	arg1	carboxylases					1215:1226	phosphoenolpyruvate carboxylases	1195:1226	phosphoenolpyruvate carboxylases (PEPCs)	1195:1234	However, most genes coding for photosystem components or those coding for phosphoenolpyruvate carboxylases (PEPCs) were upregulated in the high biomass group.
32993494	8	53	theme	biomass	1265:1271	arg1	group					1273:1277	the high biomass group	1256:1277	the high biomass group	1256:1277	However, most genes coding for photosystem components or those coding for phosphoenolpyruvate carboxylases (PEPCs) were upregulated in the high biomass group.
32993494	10	54	theme	cell	1573:1576	arg1	components					1583:1592	cell wall components	1573:1592	cell wall components	1573:1592	Genes in pathways related to biosynthesis of cell wall components and expansins coding genes showed low average expression levels and were mostly upregulated in the high biomass group.
32993494	0	55	theme	carbon	130:135	arg1	partitioning					137:148	carbon partitioning	130:148	carbon partitioning	130:148	Differential expression in leaves of Saccharum genotypes contrasting in biomass production provides evidence of genes involved in carbon partitioning.
32993494	3	56	theme	genotypes	547:555	arg1	groups					537:542	two groups	533:542	two groups of genotypes contrasting in biomass composition	533:590	To expand the understanding of the biological pathways related to these traits, we evaluated gene expression of two groups of genotypes contrasting in biomass composition.
32993494	3	56	theme	genotypes	547:555	arg1	genotypes					547:555	genotypes	547:555	genotypes contrasting in biomass composition	547:590	To expand the understanding of the biological pathways related to these traits, we evaluated gene expression of two groups of genotypes contrasting in biomass composition.
32993494	9	57	theme	sucrose	1454:1460	arg1	SPS					1482:1484	SPS	1482:1484	SPS	1482:1484	Sucrose synthase (SuSy) coding genes were upregulated in the low biomass group, showing that this enzyme class can be involved with sucrose synthesis in leaves, similarly to sucrose phosphate synthase (SPS) and sucrose phosphate phosphatase (SPP).
32993494	9	57	theme	sucrose	1454:1460	arg1	synthase					1472:1479	sucrose phosphate synthase	1454:1479	sucrose phosphate synthase (SPS)	1454:1485	Sucrose synthase (SuSy) coding genes were upregulated in the low biomass group, showing that this enzyme class can be involved with sucrose synthesis in leaves, similarly to sucrose phosphate synthase (SPS) and sucrose phosphate phosphatase (SPP).
32993494	3	58	theme	biomass	572:578	arg1	composition					580:590	biomass composition	572:590	biomass composition	572:590	To expand the understanding of the biological pathways related to these traits, we evaluated gene expression of two groups of genotypes contrasting in biomass composition.
32993494	11	59	from	differences	1754:1764	arg1	synthesis					1782:1790	carbohydrate synthesis	1769:1790	carbohydrate synthesis	1769:1790	CONCLUSIONS Together, these results show differences in carbohydrate synthesis and carbon partitioning in the source tissue of distinct phenotypic groups.
32993494	11	59	from	differences	1754:1764	arg1	partitioning					1803:1814	carbon partitioning	1796:1814	carbon partitioning	1796:1814	CONCLUSIONS Together, these results show differences in carbohydrate synthesis and carbon partitioning in the source tissue of distinct phenotypic groups.
32993494	11	59	from	differences	1754:1764	arg1	tissue					1830:1835	the source tissue	1819:1835	the source tissue of distinct phenotypic groups	1819:1865	CONCLUSIONS Together, these results show differences in carbohydrate synthesis and carbon partitioning in the source tissue of distinct phenotypic groups.
32993494	7	60	theme	substantial	1060:1070	arg1	variability					1072:1082	substantial variability	1060:1082	substantial variability in genes involved in photosynthesis	1060:1118	Genotypes within both biomass groups showed substantial variability in genes involved in photosynthesis.
32993494	4	61	dep	RESULTS	593:599	arg1	collected					634:642	collected	634:642	were collected from 12 genotypes, six per group, to perform RNA-Seq	629:695	RESULTS First visible dewlap leaves were collected from 12 genotypes, six per group, to perform RNA-Seq.
32993494	1	62	theme	yield	219:223	arg1	demands					225:231	industrial yield demands	208:231	industrial yield demands	208:231	BACKGROUND The development of biomass crops aims to meet industrial yield demands, in order to optimize profitability and sustainability.
32993494	12	63	theme	polyploid	1939:1947	arg1	system					1949:1954	a complex polyploid system	1929:1954	a complex polyploid system	1929:1954	Our data from sugarcane leaves revealed how hybridization in a complex polyploid system resulted in noticeably different transcriptomic profiles between contrasting genotypes.
32993494	10	64	theme	expansins	1598:1606	arg1	genes					1615:1619	expansins coding genes	1598:1619	expansins coding genes	1598:1619	Genes in pathways related to biosynthesis of cell wall components and expansins coding genes showed low average expression levels and were mostly upregulated in the high biomass group.
32993494	9	65	theme	Sucrose	1280:1286	arg1	SuSy					1298:1301	SuSy	1298:1301	SuSy	1298:1301	Sucrose synthase (SuSy) coding genes were upregulated in the low biomass group, showing that this enzyme class can be involved with sucrose synthesis in leaves, similarly to sucrose phosphate synthase (SPS) and sucrose phosphate phosphatase (SPP).
32993494	9	65	theme	Sucrose	1280:1286	arg1	synthase					1288:1295	Sucrose synthase	1280:1295	Sucrose synthase (SuSy) coding genes	1280:1315	Sucrose synthase (SuSy) coding genes were upregulated in the low biomass group, showing that this enzyme class can be involved with sucrose synthesis in leaves, similarly to sucrose phosphate synthase (SPS) and sucrose phosphate phosphatase (SPP).
32993494	5	66	theme	expressed	739:747	arg1	genes					749:753	differentially expressed genes	724:753	differentially expressed genes	724:753	We found a high number of differentially expressed genes, showing how hybridization in a complex polyploid system caused extensive modifications in genome functioning.
32993494	5	67	theme	extensive	819:827	arg1	modifications					829:841	extensive modifications	819:841	extensive modifications	819:841	We found a high number of differentially expressed genes, showing how hybridization in a complex polyploid system caused extensive modifications in genome functioning.
32993494	9	68	theme	phosphate	1499:1507	arg1	phosphatase					1509:1519	sucrose phosphate phosphatase	1491:1519	sucrose phosphate phosphatase (SPP)	1491:1525	Sucrose synthase (SuSy) coding genes were upregulated in the low biomass group, showing that this enzyme class can be involved with sucrose synthesis in leaves, similarly to sucrose phosphate synthase (SPS) and sucrose phosphate phosphatase (SPP).
32993494	9	68	theme	phosphate	1499:1507	arg1	SPP					1522:1524	SPP	1522:1524	SPP	1522:1524	Sucrose synthase (SuSy) coding genes were upregulated in the low biomass group, showing that this enzyme class can be involved with sucrose synthesis in leaves, similarly to sucrose phosphate synthase (SPS) and sucrose phosphate phosphatase (SPP).
32993494	12	69	theme	transcriptomic	1989:2002	arg1	profiles					2004:2011	noticeably different transcriptomic profiles	1968:2011	noticeably different transcriptomic profiles between contrasting genotypes	1968:2041	Our data from sugarcane leaves revealed how hybridization in a complex polyploid system resulted in noticeably different transcriptomic profiles between contrasting genotypes.
32993494	6	70	theme	Saccharum	997:1005	arg1	genomes					1007:1013	the polyploid Saccharum genomes	983:1013	the polyploid Saccharum genomes	983:1013	We found evidence that differences in transposition and defense related genes may arise due to the complex nature of the polyploid Saccharum genomes.
32993494	0	71	from	expression	13:22	arg1	leaves					27:32	leaves	27:32	leaves	27:32	Differential expression in leaves of Saccharum genotypes contrasting in biomass production provides evidence of genes involved in carbon partitioning.
32993494	11	72	theme	carbohydrate	1769:1780	arg1	synthesis					1782:1790	carbohydrate synthesis	1769:1790	carbohydrate synthesis	1769:1790	CONCLUSIONS Together, these results show differences in carbohydrate synthesis and carbon partitioning in the source tissue of distinct phenotypic groups.
32993494	5	73	theme	high	709:712	arg1	number					714:719	a high number	707:719	a high number of differentially expressed genes	707:753	We found a high number of differentially expressed genes, showing how hybridization in a complex polyploid system caused extensive modifications in genome functioning.
32993494	5	74	from	hybridization	768:780	arg1	system					805:810	a complex polyploid system	785:810	a complex polyploid system	785:810	We found a high number of differentially expressed genes, showing how hybridization in a complex polyploid system caused extensive modifications in genome functioning.
32993494	12	75	theme	complex	1931:1937	arg1	system					1949:1954	a complex polyploid system	1929:1954	a complex polyploid system	1929:1954	Our data from sugarcane leaves revealed how hybridization in a complex polyploid system resulted in noticeably different transcriptomic profiles between contrasting genotypes.
32993494	9	76	theme	phosphate	1462:1470	arg1	SPS					1482:1484	SPS	1482:1484	SPS	1482:1484	Sucrose synthase (SuSy) coding genes were upregulated in the low biomass group, showing that this enzyme class can be involved with sucrose synthesis in leaves, similarly to sucrose phosphate synthase (SPS) and sucrose phosphate phosphatase (SPP).
32993494	9	76	theme	phosphate	1462:1470	arg1	synthase					1472:1479	sucrose phosphate synthase	1454:1479	sucrose phosphate synthase (SPS)	1454:1485	Sucrose synthase (SuSy) coding genes were upregulated in the low biomass group, showing that this enzyme class can be involved with sucrose synthesis in leaves, similarly to sucrose phosphate synthase (SPS) and sucrose phosphate phosphatase (SPP).
32993494	10	77	theme	average	1632:1638	arg1	levels					1651:1656	low average expression levels	1628:1656	low average expression levels	1628:1656	Genes in pathways related to biosynthesis of cell wall components and expansins coding genes showed low average expression levels and were mostly upregulated in the high biomass group.
32993494	5	78	theme	complex	787:793	arg1	system					805:810	a complex polyploid system	785:810	a complex polyploid system	785:810	We found a high number of differentially expressed genes, showing how hybridization in a complex polyploid system caused extensive modifications in genome functioning.
32993494	9	79	theme	biomass	1345:1351	arg1	group					1353:1357	the low biomass group	1337:1357	the low biomass group	1337:1357	Sucrose synthase (SuSy) coding genes were upregulated in the low biomass group, showing that this enzyme class can be involved with sucrose synthesis in leaves, similarly to sucrose phosphate synthase (SPS) and sucrose phosphate phosphatase (SPP).
32993494	9	80	from	synthesis	1420:1428	arg1	leaves					1433:1438	leaves	1433:1438	leaves	1433:1438	Sucrose synthase (SuSy) coding genes were upregulated in the low biomass group, showing that this enzyme class can be involved with sucrose synthesis in leaves, similarly to sucrose phosphate synthase (SPS) and sucrose phosphate phosphatase (SPP).
32993494	3	81	theme	related	476:482	arg1	pathways					467:474	the biological pathways	452:474	the biological pathways related to these traits	452:498	To expand the understanding of the biological pathways related to these traits, we evaluated gene expression of two groups of genotypes contrasting in biomass composition.
32993494	0	82	theme	Saccharum	37:45	arg1	genotypes					47:55	Saccharum genotypes	37:55	Saccharum genotypes contrasting in biomass production	37:89	Differential expression in leaves of Saccharum genotypes contrasting in biomass production provides evidence of genes involved in carbon partitioning.
32993494	3	83	theme	biological	456:465	arg1	pathways					467:474	the biological pathways	452:474	the biological pathways related to these traits	452:498	To expand the understanding of the biological pathways related to these traits, we evaluated gene expression of two groups of genotypes contrasting in biomass composition.
32993494	10	84	from	Genes	1528:1532	arg1	pathways					1537:1544	pathways	1537:1544	pathways related to biosynthesis of cell wall components	1537:1592	Genes in pathways related to biosynthesis of cell wall components and expansins coding genes showed low average expression levels and were mostly upregulated in the high biomass group.
32993494	11	85	theme	groups	1860:1865	arg1	tissue					1830:1835	the source tissue	1819:1835	the source tissue of distinct phenotypic groups	1819:1865	CONCLUSIONS Together, these results show differences in carbohydrate synthesis and carbon partitioning in the source tissue of distinct phenotypic groups.
32993494	9	86	theme	enzyme	1378:1383	arg1	class					1385:1389	this enzyme class	1373:1389	this enzyme class	1373:1389	Sucrose synthase (SuSy) coding genes were upregulated in the low biomass group, showing that this enzyme class can be involved with sucrose synthesis in leaves, similarly to sucrose phosphate synthase (SPS) and sucrose phosphate phosphatase (SPP).
32993494	11	87	theme	distinct	1840:1847	arg1	groups					1860:1865	distinct phenotypic groups	1840:1865	distinct phenotypic groups	1840:1865	CONCLUSIONS Together, these results show differences in carbohydrate synthesis and carbon partitioning in the source tissue of distinct phenotypic groups.
32993494	2	88	theme	sucrose	367:373	arg1	accumulation					375:386	sucrose accumulation	367:386	sucrose accumulation	367:386	Achieving these goals in an energy crop like sugarcane relies on breeding for sucrose accumulation, fiber content and stalk number.
33625776	9	0	theme	oxylipin	1609:1616	arg1	incorporation					1618:1630	oxylipin incorporation	1609:1630	oxylipin incorporation into VLDL	1609:1640	We conclude that the liver regulates oxylipin incorporation into VLDL, and the oxylipin content is altered by LPS challenge and by inhibition of the epoxide hydrolase pathway.
33625776	1	1	theme	vascular	279:286	arg1	tone					288:291	vascular tone	279:291	vascular tone	279:291	Oxylipins are metabolites of polyunsaturated fatty acids that mediate cardiovascular health by attenuation of inflammation, vascular tone, hemostasis, and thrombosis.
33625776	6	2	link	EPA-derived	1168:1178	arg1	epoxide					1180:1186	the EPA-derived epoxide	1164:1186	the EPA-derived epoxide	1164:1186	However, the EPA-derived epoxide, 17(18)-EpETE, was decreased by 75% [49%,88%], (-0.52 nM) with LPS, double the suppression of other oxylipins.
33625776	6	2	link	EPA-derived	1168:1178	arg1	-EpETE					1195:1200	17(18)-EpETE	1189:1200	17(18)-EpETE	1189:1200	However, the EPA-derived epoxide, 17(18)-EpETE, was decreased by 75% [49%,88%], (-0.52 nM) with LPS, double the suppression of other oxylipins.
33625776	7	3	theme	epoxide	1341:1347	arg1	concentration					1320:1332	final concentration	1314:1332	final concentration of DHA epoxide, 16(17)-EpDPE, by 99% [35%,193%], (2.0 nM)	1314:1390	sEHi increased final concentration of DHA epoxide, 16(17)-EpDPE, by 99% [35%,193%], (2.0 nM).
33625776	3	4	from	effect	507:512	arg1	oxylipins					621:629	VLDL oxylipins	616:629	VLDL oxylipins	616:629	In this study, we used a perfused liver model to observe the effect of inflammatory lipopolysaccharide (LPS) challenge and soluble epoxide hydrolase inhibition (sEHi) on VLDL oxylipins.
33625776	6	5	theme	EPA-derived	1168:1178	arg1	epoxide					1180:1186	the EPA-derived epoxide	1164:1186	the EPA-derived epoxide	1164:1186	However, the EPA-derived epoxide, 17(18)-EpETE, was decreased by 75% [49%,88%], (-0.52 nM) with LPS, double the suppression of other oxylipins.
33625776	6	5	theme	EPA-derived	1168:1178	arg1	-EpETE					1195:1200	17(18)-EpETE	1189:1200	17(18)-EpETE	1189:1200	However, the EPA-derived epoxide, 17(18)-EpETE, was decreased by 75% [49%,88%], (-0.52 nM) with LPS, double the suppression of other oxylipins.
33625776	10	6	theme	systemic	1787:1794	arg1	signals					1805:1811	systemic oxylipin signals	1787:1811	systemic oxylipin signals	1787:1811	This provides evidence for delivery of systemic oxylipin signals by VLDL transport.
33625776	7	7	dep	-EpDPE	1356:1361	arg1	%					1379:1379	193%	1376:1379	193%	1376:1379	sEHi increased final concentration of DHA epoxide, 16(17)-EpDPE, by 99% [35%,193%], (2.0 nM).
33625776	3	8	theme	lipopolysaccharide	530:547	arg1	challenge					555:563	inflammatory lipopolysaccharide (LPS) challenge	517:563	inflammatory lipopolysaccharide (LPS) challenge	517:563	In this study, we used a perfused liver model to observe the effect of inflammatory lipopolysaccharide (LPS) challenge and soluble epoxide hydrolase inhibition (sEHi) on VLDL oxylipins.
33625776	1	9	theme	polyunsaturated	184:198	arg1	acids					206:210	polyunsaturated fatty acids	184:210	polyunsaturated fatty acids	184:210	Oxylipins are metabolites of polyunsaturated fatty acids that mediate cardiovascular health by attenuation of inflammation, vascular tone, hemostasis, and thrombosis.
33625776	7	10	theme	final	1314:1318	arg1	concentration					1320:1332	final concentration	1314:1332	final concentration of DHA epoxide, 16(17)-EpDPE, by 99% [35%,193%], (2.0 nM)	1314:1390	sEHi increased final concentration of DHA epoxide, 16(17)-EpDPE, by 99% [35%,193%], (2.0 nM).
33625776	4	11	theme	linoleic	675:682	arg1	acid					684:687	linoleic acid	675:687	linoleic acid	675:687	A compartmental model of deuterium-labeled linoleic acid and palmitic acid incorporation into VLDL was also developed to assess the dependence of VLDL oxylipins on fatty acid incorporation rates.
33625776	2	12	theme	low-density	327:337	arg1	VLDL					353:356	VLDL	353:356	VLDL	353:356	Very low-density lipoproteins (VLDL) contain oxylipins, but it is unknown whether the liver regulates their concentrations.
33625776	2	12	theme	low-density	327:337	arg1	lipoproteins					339:350	Very low-density lipoproteins	322:350	Very low-density lipoproteins (VLDL)	322:357	Very low-density lipoproteins (VLDL) contain oxylipins, but it is unknown whether the liver regulates their concentrations.
33625776	1	13	theme	acids	206:210	arg1	Oxylipins					155:163	Oxylipins	155:163	Oxylipins	155:163	Oxylipins are metabolites of polyunsaturated fatty acids that mediate cardiovascular health by attenuation of inflammation, vascular tone, hemostasis, and thrombosis.
33625776	1	13	theme	acids	206:210	arg1	metabolites					169:179	metabolites	169:179	metabolites of polyunsaturated fatty acids that mediate cardiovascular health by attenuation of inflammation, vascular tone, hemostasis, and thrombosis	169:319	Oxylipins are metabolites of polyunsaturated fatty acids that mediate cardiovascular health by attenuation of inflammation, vascular tone, hemostasis, and thrombosis.
33625776	10	14	theme	VLDL	1816:1819	arg1	transport					1821:1829	VLDL transport	1816:1829	VLDL transport	1816:1829	This provides evidence for delivery of systemic oxylipin signals by VLDL transport.
33625776	0	15	theme	isolated	125:132	arg1	livers					147:152	isolated perfused rat livers	125:152	isolated perfused rat livers	125:152	Effects of inflammation and soluble epoxide hydrolase inhibition on oxylipin composition of very low-density lipoproteins in isolated perfused rat livers.
33625776	3	16	theme	challenge	555:563	arg1	effect					507:512	the effect	503:512	the effect of inflammatory lipopolysaccharide (LPS) challenge and soluble epoxide hydrolase inhibition (sEHi) on VLDL oxylipins	503:629	In this study, we used a perfused liver model to observe the effect of inflammatory lipopolysaccharide (LPS) challenge and soluble epoxide hydrolase inhibition (sEHi) on VLDL oxylipins.
33625776	10	17	theme	signals	1805:1811	arg1	delivery					1775:1782	delivery	1775:1782	delivery of systemic oxylipin signals by VLDL transport	1775:1829	This provides evidence for delivery of systemic oxylipin signals by VLDL transport.
33625776	0	18	from	composition	77:87	arg1	livers					147:152	isolated perfused rat livers	125:152	isolated perfused rat livers	125:152	Effects of inflammation and soluble epoxide hydrolase inhibition on oxylipin composition of very low-density lipoproteins in isolated perfused rat livers.
33625776	4	19	theme	compartmental	634:646	arg1	model					648:652	A compartmental model	632:652	A compartmental model of deuterium-labeled linoleic acid and palmitic acid incorporation into VLDL	632:729	A compartmental model of deuterium-labeled linoleic acid and palmitic acid incorporation into VLDL was also developed to assess the dependence of VLDL oxylipins on fatty acid incorporation rates.
33625776	0	20	theme	rat	143:145	arg1	livers					147:152	isolated perfused rat livers	125:152	isolated perfused rat livers	125:152	Effects of inflammation and soluble epoxide hydrolase inhibition on oxylipin composition of very low-density lipoproteins in isolated perfused rat livers.
33625776	5	21	theme	fatty	852:856	arg1	content					868:874	the total fatty acid VLDL content	842:874	the total fatty acid VLDL content	842:874	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	9	22	theme	LPS	1682:1684	arg1	challenge					1686:1694	LPS challenge	1682:1694	LPS challenge	1682:1694	We conclude that the liver regulates oxylipin incorporation into VLDL, and the oxylipin content is altered by LPS challenge and by inhibition of the epoxide hydrolase pathway.
33625776	5	23	theme	%	981:981	arg1	%					985:985	35% [4%	979:985	35% [4%	979:985	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	5	23	theme	%	981:981	arg1	13-HOTrE					969:976	13-HOTrE	969:976	13-HOTrE	969:976	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	3	24	theme	hydrolase	585:593	arg1	inhibition					595:604	soluble epoxide hydrolase inhibition	569:604	soluble epoxide hydrolase inhibition (sEHi)	569:611	In this study, we used a perfused liver model to observe the effect of inflammatory lipopolysaccharide (LPS) challenge and soluble epoxide hydrolase inhibition (sEHi) on VLDL oxylipins.
33625776	3	24	theme	hydrolase	585:593	arg1	sEHi					607:610	sEHi	607:610	sEHi	607:610	In this study, we used a perfused liver model to observe the effect of inflammatory lipopolysaccharide (LPS) challenge and soluble epoxide hydrolase inhibition (sEHi) on VLDL oxylipins.
33625776	0	25	from	lipoproteins	109:120	arg1	livers					147:152	isolated perfused rat livers	125:152	isolated perfused rat livers	125:152	Effects of inflammation and soluble epoxide hydrolase inhibition on oxylipin composition of very low-density lipoproteins in isolated perfused rat livers.
33625776	8	26	theme	VLDL-oxylipin	1399:1411	arg1	concentrations					1413:1426	Final VLDL-oxylipin concentrations	1393:1426	Final VLDL-oxylipin concentrations with LPS treatment	1393:1445	Final VLDL-oxylipin concentrations with LPS treatment were not correlated with linoleic acid kinetics, suggesting they were independently regulated under inflammatory conditions.
33625776	3	27	theme	VLDL	616:619	arg1	oxylipins					621:629	VLDL oxylipins	616:629	VLDL oxylipins	616:629	In this study, we used a perfused liver model to observe the effect of inflammatory lipopolysaccharide (LPS) challenge and soluble epoxide hydrolase inhibition (sEHi) on VLDL oxylipins.
33625776	5	28	dep	decreased	897:905	arg1	-1.3 nM					993:999	-1.3 nM	993:999	-1.3 nM	993:999	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	6	29	theme	other	1282:1286	arg1	oxylipins					1288:1296	other oxylipins	1282:1296	other oxylipins	1282:1296	However, the EPA-derived epoxide, 17(18)-EpETE, was decreased by 75% [49%,88%], (-0.52 nM) with LPS, double the suppression of other oxylipins.
33625776	4	30	theme	acid	702:705	arg1	incorporation					707:719	deuterium-labeled linoleic acid and palmitic acid incorporation	657:719	deuterium-labeled linoleic acid and palmitic acid incorporation into VLDL	657:729	A compartmental model of deuterium-labeled linoleic acid and palmitic acid incorporation into VLDL was also developed to assess the dependence of VLDL oxylipins on fatty acid incorporation rates.
33625776	5	31	theme	[2	1056:1057	arg1	%					1058:1058	29% [2%	1052:1058	29% [2%,49%]	1052:1063	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	5	31	theme	[2	1056:1057	arg1	-EpODE					1044:1049	15(16)-EpODE	1038:1049	15(16)-EpODE	1038:1049	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	9	32	theme	hydrolase	1729:1737	arg1	pathway					1739:1745	the epoxide hydrolase pathway	1717:1745	the epoxide hydrolase pathway	1717:1745	We conclude that the liver regulates oxylipin incorporation into VLDL, and the oxylipin content is altered by LPS challenge and by inhibition of the epoxide hydrolase pathway.
33625776	4	33	from	dependence	764:773	arg1	rates					821:825	fatty acid incorporation rates	796:825	fatty acid incorporation rates	796:825	A compartmental model of deuterium-labeled linoleic acid and palmitic acid incorporation into VLDL was also developed to assess the dependence of VLDL oxylipins on fatty acid incorporation rates.
33625776	5	34	dep	%	1021:1021	arg1	%					1025:1025	49%	1023:1025	49%	1023:1025	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	4	35	theme	VLDL	778:781	arg1	oxylipins					783:791	VLDL oxylipins	778:791	VLDL oxylipins	778:791	A compartmental model of deuterium-labeled linoleic acid and palmitic acid incorporation into VLDL was also developed to assess the dependence of VLDL oxylipins on fatty acid incorporation rates.
33625776	0	36	theme	lipoproteins	109:120	arg1	composition					77:87	oxylipin composition	68:87	oxylipin composition of very low-density lipoproteins in isolated perfused rat livers	68:152	Effects of inflammation and soluble epoxide hydrolase inhibition on oxylipin composition of very low-density lipoproteins in isolated perfused rat livers.
33625776	5	37	theme	oxylipins	938:946	arg1	concentration					913:925	final concentration	907:925	final concentration of several oxylipins	907:946	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	8	38	theme	LPS	1433:1435	arg1	treatment					1437:1445	LPS treatment	1433:1445	LPS treatment	1433:1445	Final VLDL-oxylipin concentrations with LPS treatment were not correlated with linoleic acid kinetics, suggesting they were independently regulated under inflammatory conditions.
33625776	8	39	theme	acid	1481:1484	arg1	kinetics					1486:1493	linoleic acid kinetics	1472:1493	linoleic acid kinetics	1472:1493	Final VLDL-oxylipin concentrations with LPS treatment were not correlated with linoleic acid kinetics, suggesting they were independently regulated under inflammatory conditions.
33625776	1	40	theme	hemostasis	294:303	arg1	attenuation					250:260	attenuation	250:260	attenuation of inflammation, vascular tone, hemostasis, and thrombosis	250:319	Oxylipins are metabolites of polyunsaturated fatty acids that mediate cardiovascular health by attenuation of inflammation, vascular tone, hemostasis, and thrombosis.
33625776	5	41	dep	%	1099:1099	arg1	%					1103:1103	52%	1101:1103	52%	1101:1103	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	0	42	theme	inflammation	11:22	arg1	Effects					0:6	Effects	0:6	Effects of inflammation	0:22	Effects of inflammation and soluble epoxide hydrolase inhibition on oxylipin composition of very low-density lipoproteins in isolated perfused rat livers.
33625776	0	42	theme	inflammation	11:22	arg1	hydrolase					44:52	soluble epoxide hydrolase	28:52	soluble epoxide hydrolase	28:52	Effects of inflammation and soluble epoxide hydrolase inhibition on oxylipin composition of very low-density lipoproteins in isolated perfused rat livers.
33625776	5	43	theme	final	907:911	arg1	concentration					913:925	final concentration	907:925	final concentration of several oxylipins	907:946	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	0	44	theme	soluble	28:34	arg1	hydrolase					44:52	soluble epoxide hydrolase	28:52	soluble epoxide hydrolase	28:52	Effects of inflammation and soluble epoxide hydrolase inhibition on oxylipin composition of very low-density lipoproteins in isolated perfused rat livers.
33625776	3	45	theme	perfused	471:478	arg1	model					486:490	a perfused liver model	469:490	a perfused liver model	469:490	In this study, we used a perfused liver model to observe the effect of inflammatory lipopolysaccharide (LPS) challenge and soluble epoxide hydrolase inhibition (sEHi) on VLDL oxylipins.
33625776	5	46	dep	decreased	832:840	arg1	%					889:889	47%	887:889	47%	887:889	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	5	47	theme	VLDL	863:866	arg1	content					868:874	the total fatty acid VLDL content	842:874	the total fatty acid VLDL content	842:874	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	7	48	theme	DHA	1337:1339	arg1	2.0 nM					1384:1389	2.0 nM	1384:1389	2.0 nM	1384:1389	sEHi increased final concentration of DHA epoxide, 16(17)-EpDPE, by 99% [35%,193%], (2.0 nM).
33625776	7	48	theme	DHA	1337:1339	arg1	-EpDPE					1356:1361	16(17)-EpDPE	1350:1361	16(17)-EpDPE, by 99% [35%,193%]	1350:1380	sEHi increased final concentration of DHA epoxide, 16(17)-EpDPE, by 99% [35%,193%], (2.0 nM).
33625776	7	48	theme	DHA	1337:1339	arg1	epoxide					1341:1347	DHA epoxide	1337:1347	DHA epoxide	1337:1347	sEHi increased final concentration of DHA epoxide, 16(17)-EpDPE, by 99% [35%,193%], (2.0 nM).
33625776	1	49	theme	tone	288:291	arg1	attenuation					250:260	attenuation	250:260	attenuation of inflammation, vascular tone, hemostasis, and thrombosis	250:319	Oxylipins are metabolites of polyunsaturated fatty acids that mediate cardiovascular health by attenuation of inflammation, vascular tone, hemostasis, and thrombosis.
33625776	5	50	dep	%	1058:1058	arg1	%					1062:1062	49%	1060:1062	49%	1060:1062	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	3	51	theme	inflammatory	517:528	arg1	LPS					550:552	LPS	550:552	LPS	550:552	In this study, we used a perfused liver model to observe the effect of inflammatory lipopolysaccharide (LPS) challenge and soluble epoxide hydrolase inhibition (sEHi) on VLDL oxylipins.
33625776	3	51	theme	inflammatory	517:528	arg1	lipopolysaccharide					530:547	inflammatory lipopolysaccharide	517:547	inflammatory lipopolysaccharide (LPS) challenge	517:563	In this study, we used a perfused liver model to observe the effect of inflammatory lipopolysaccharide (LPS) challenge and soluble epoxide hydrolase inhibition (sEHi) on VLDL oxylipins.
33625776	5	52	theme	acid	858:861	arg1	content					868:874	the total fatty acid VLDL content	842:874	the total fatty acid VLDL content	842:874	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	1	53	theme	fatty	200:204	arg1	acids					206:210	polyunsaturated fatty acids	184:210	polyunsaturated fatty acids	184:210	Oxylipins are metabolites of polyunsaturated fatty acids that mediate cardiovascular health by attenuation of inflammation, vascular tone, hemostasis, and thrombosis.
33625776	4	54	theme	deuterium-labeled	657:673	arg1	incorporation					707:719	deuterium-labeled linoleic acid and palmitic acid incorporation	657:719	deuterium-labeled linoleic acid and palmitic acid incorporation into VLDL	657:729	A compartmental model of deuterium-labeled linoleic acid and palmitic acid incorporation into VLDL was also developed to assess the dependence of VLDL oxylipins on fatty acid incorporation rates.
33625776	9	55	theme	oxylipin	1651:1658	arg1	content					1660:1666	the oxylipin content	1647:1666	the oxylipin content	1647:1666	We conclude that the liver regulates oxylipin incorporation into VLDL, and the oxylipin content is altered by LPS challenge and by inhibition of the epoxide hydrolase pathway.
33625776	0	56	theme	perfused	134:141	arg1	livers					147:152	isolated perfused rat livers	125:152	isolated perfused rat livers	125:152	Effects of inflammation and soluble epoxide hydrolase inhibition on oxylipin composition of very low-density lipoproteins in isolated perfused rat livers.
33625776	3	57	theme	soluble	569:575	arg1	hydrolase					585:593	soluble epoxide hydrolase	569:593	soluble epoxide hydrolase inhibition (sEHi)	569:611	In this study, we used a perfused liver model to observe the effect of inflammatory lipopolysaccharide (LPS) challenge and soluble epoxide hydrolase inhibition (sEHi) on VLDL oxylipins.
33625776	5	58	dep	amount	961:966	arg1	%					985:985	35% [4%	979:985	35% [4%	979:985	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	5	58	dep	amount	961:966	arg1	13-HOTrE					969:976	13-HOTrE	969:976	13-HOTrE	969:976	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	5	58	dep	amount	961:966	arg1	%					989:989	55%	987:989	55%	987:989	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	3	59	theme	inhibition	595:604	arg1	effect					507:512	the effect	503:512	the effect of inflammatory lipopolysaccharide (LPS) challenge and soluble epoxide hydrolase inhibition (sEHi) on VLDL oxylipins	503:629	In this study, we used a perfused liver model to observe the effect of inflammatory lipopolysaccharide (LPS) challenge and soluble epoxide hydrolase inhibition (sEHi) on VLDL oxylipins.
33625776	5	60	theme	total	846:850	arg1	content					868:874	the total fatty acid VLDL content	842:874	the total fatty acid VLDL content	842:874	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	10	61	theme	oxylipin	1796:1803	arg1	signals					1805:1811	systemic oxylipin signals	1787:1811	systemic oxylipin signals	1787:1811	This provides evidence for delivery of systemic oxylipin signals by VLDL transport.
33625776	8	62	theme	Final	1393:1397	arg1	concentrations					1413:1426	Final VLDL-oxylipin concentrations	1393:1426	Final VLDL-oxylipin concentrations with LPS treatment	1393:1445	Final VLDL-oxylipin concentrations with LPS treatment were not correlated with linoleic acid kinetics, suggesting they were independently regulated under inflammatory conditions.
33625776	3	63	theme	epoxide	577:583	arg1	hydrolase					585:593	soluble epoxide hydrolase	569:593	soluble epoxide hydrolase inhibition (sEHi)	569:611	In this study, we used a perfused liver model to observe the effect of inflammatory lipopolysaccharide (LPS) challenge and soluble epoxide hydrolase inhibition (sEHi) on VLDL oxylipins.
33625776	4	64	theme	incorporation	707:719	arg1	model					648:652	A compartmental model	632:652	A compartmental model of deuterium-labeled linoleic acid and palmitic acid incorporation into VLDL	632:729	A compartmental model of deuterium-labeled linoleic acid and palmitic acid incorporation into VLDL was also developed to assess the dependence of VLDL oxylipins on fatty acid incorporation rates.
33625776	0	65	theme	oxylipin	68:75	arg1	composition					77:87	oxylipin composition	68:87	oxylipin composition of very low-density lipoproteins in isolated perfused rat livers	68:152	Effects of inflammation and soluble epoxide hydrolase inhibition on oxylipin composition of very low-density lipoproteins in isolated perfused rat livers.
33625776	5	66	theme	30	879:880	arg1	%					881:881	%	881:881	%	881:881	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	6	67	theme	oxylipins	1288:1296	arg1	LPS					1251:1253	LPS	1251:1253	LPS	1251:1253	However, the EPA-derived epoxide, 17(18)-EpETE, was decreased by 75% [49%,88%], (-0.52 nM) with LPS, double the suppression of other oxylipins.
33625776	6	67	theme	oxylipins	1288:1296	arg1	suppression					1267:1277	double the suppression	1256:1277	double the suppression of other oxylipins	1256:1296	However, the EPA-derived epoxide, 17(18)-EpETE, was decreased by 75% [49%,88%], (-0.52 nM) with LPS, double the suppression of other oxylipins.
33625776	4	68	theme	acid	684:687	arg1	incorporation					707:719	deuterium-labeled linoleic acid and palmitic acid incorporation	657:719	deuterium-labeled linoleic acid and palmitic acid incorporation into VLDL	657:729	A compartmental model of deuterium-labeled linoleic acid and palmitic acid incorporation into VLDL was also developed to assess the dependence of VLDL oxylipins on fatty acid incorporation rates.
33625776	8	69	with	concentrations	1413:1426	arg1	treatment					1437:1445	LPS treatment	1433:1445	LPS treatment	1433:1445	Final VLDL-oxylipin concentrations with LPS treatment were not correlated with linoleic acid kinetics, suggesting they were independently regulated under inflammatory conditions.
33625776	5	70	theme	[6	883:884	arg1	%					881:881	%	881:881	%	881:881	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	4	71	theme	palmitic	693:700	arg1	acid					702:705	palmitic acid	693:705	palmitic acid	693:705	A compartmental model of deuterium-labeled linoleic acid and palmitic acid incorporation into VLDL was also developed to assess the dependence of VLDL oxylipins on fatty acid incorporation rates.
33625776	5	72	theme	%	1054:1054	arg1	%					1058:1058	29% [2%	1052:1058	29% [2%,49%]	1052:1063	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	5	72	theme	%	1054:1054	arg1	-EpODE					1044:1049	15(16)-EpODE	1038:1049	15(16)-EpODE	1038:1049	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	9	73	theme	epoxide	1721:1727	arg1	pathway					1739:1745	the epoxide hydrolase pathway	1717:1745	the epoxide hydrolase pathway	1717:1745	We conclude that the liver regulates oxylipin incorporation into VLDL, and the oxylipin content is altered by LPS challenge and by inhibition of the epoxide hydrolase pathway.
33625776	0	74	theme	low-density	97:107	arg1	lipoproteins					109:120	very low-density lipoproteins	92:120	very low-density lipoproteins in isolated perfused rat livers	92:152	Effects of inflammation and soluble epoxide hydrolase inhibition on oxylipin composition of very low-density lipoproteins in isolated perfused rat livers.
33625776	2	75	contain	contain	359:365	arg1	VLDL					353:356	VLDL	353:356	VLDL	353:356	Very low-density lipoproteins (VLDL) contain oxylipins, but it is unknown whether the liver regulates their concentrations.
33625776	2	75	contain	contain	359:365	arg2	oxylipins					367:375	oxylipins	367:375	oxylipins	367:375	Very low-density lipoproteins (VLDL) contain oxylipins, but it is unknown whether the liver regulates their concentrations.
33625776	2	75	contain	contain	359:365	arg1	lipoproteins					339:350	Very low-density lipoproteins	322:350	Very low-density lipoproteins (VLDL)	322:357	Very low-density lipoproteins (VLDL) contain oxylipins, but it is unknown whether the liver regulates their concentrations.
33625776	0	76	from	inhibition	54:63	arg1	composition					77:87	oxylipin composition	68:87	oxylipin composition of very low-density lipoproteins in isolated perfused rat livers	68:152	Effects of inflammation and soluble epoxide hydrolase inhibition on oxylipin composition of very low-density lipoproteins in isolated perfused rat livers.
33625776	5	77	theme	AA-derived	1075:1084	arg1	diols					1086:1090	AA-derived diols	1075:1090	AA-derived diols	1075:1090	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	9	78	theme	pathway	1739:1745	arg1	inhibition					1703:1712	inhibition	1703:1712	inhibition of the epoxide hydrolase pathway	1703:1745	We conclude that the liver regulates oxylipin incorporation into VLDL, and the oxylipin content is altered by LPS challenge and by inhibition of the epoxide hydrolase pathway.
33625776	8	79	theme	linoleic	1472:1479	arg1	kinetics					1486:1493	linoleic acid kinetics	1472:1493	linoleic acid kinetics	1472:1493	Final VLDL-oxylipin concentrations with LPS treatment were not correlated with linoleic acid kinetics, suggesting they were independently regulated under inflammatory conditions.
33625776	6	80	theme	%	1222:1222	arg1	-0.52 nM					1236:1243	-0.52 nM	1236:1243	-0.52 nM	1236:1243	However, the EPA-derived epoxide, 17(18)-EpETE, was decreased by 75% [49%,88%], (-0.52 nM) with LPS, double the suppression of other oxylipins.
33625776	6	80	theme	%	1222:1222	arg1	%					1227:1227	75% [49%	1220:1227	75% [49%	1220:1227	However, the EPA-derived epoxide, 17(18)-EpETE, was decreased by 75% [49%,88%], (-0.52 nM) with LPS, double the suppression of other oxylipins.
33625776	6	80	theme	%	1222:1222	arg1	%					1231:1231	88%]	1229:1232	88%]	1229:1232	However, the EPA-derived epoxide, 17(18)-EpETE, was decreased by 75% [49%,88%], (-0.52 nM) with LPS, double the suppression of other oxylipins.
33625776	5	81	theme	several	930:936	arg1	oxylipins					938:946	several oxylipins	930:946	several oxylipins	930:946	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	8	82	theme	inflammatory	1547:1558	arg1	conditions					1560:1569	inflammatory conditions	1547:1569	inflammatory conditions	1547:1569	Final VLDL-oxylipin concentrations with LPS treatment were not correlated with linoleic acid kinetics, suggesting they were independently regulated under inflammatory conditions.
33625776	5	83	link	AA-derived	1075:1084	arg1	diols					1086:1090	AA-derived diols	1075:1090	AA-derived diols	1075:1090	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	5	84	theme	%	1133:1133	arg1	%					1137:1137	31% [7%	1131:1137	31% [7%	1131:1137	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	6	85	theme	double	1256:1261	arg1	LPS					1251:1253	LPS	1251:1253	LPS	1251:1253	However, the EPA-derived epoxide, 17(18)-EpETE, was decreased by 75% [49%,88%], (-0.52 nM) with LPS, double the suppression of other oxylipins.
33625776	6	85	theme	double	1256:1261	arg1	suppression					1267:1277	double the suppression	1256:1277	double the suppression of other oxylipins	1256:1296	However, the EPA-derived epoxide, 17(18)-EpETE, was decreased by 75% [49%,88%], (-0.52 nM) with LPS, double the suppression of other oxylipins.
33625776	4	86	theme	acid	802:805	arg1	rates					821:825	fatty acid incorporation rates	796:825	fatty acid incorporation rates	796:825	A compartmental model of deuterium-labeled linoleic acid and palmitic acid incorporation into VLDL was also developed to assess the dependence of VLDL oxylipins on fatty acid incorporation rates.
33625776	3	87	used	used	464:467	arg2	we					461:462	we	461:462	we	461:462	In this study, we used a perfused liver model to observe the effect of inflammatory lipopolysaccharide (LPS) challenge and soluble epoxide hydrolase inhibition (sEHi) on VLDL oxylipins.
33625776	0	88	theme	epoxide	36:42	arg1	hydrolase					44:52	soluble epoxide hydrolase	28:52	soluble epoxide hydrolase	28:52	Effects of inflammation and soluble epoxide hydrolase inhibition on oxylipin composition of very low-density lipoproteins in isolated perfused rat livers.
33625776	1	89	theme	thrombosis	310:319	arg1	attenuation					250:260	attenuation	250:260	attenuation of inflammation, vascular tone, hemostasis, and thrombosis	250:319	Oxylipins are metabolites of polyunsaturated fatty acids that mediate cardiovascular health by attenuation of inflammation, vascular tone, hemostasis, and thrombosis.
33625776	1	90	theme	cardiovascular	225:238	arg1	health					240:245	cardiovascular health	225:245	cardiovascular health	225:245	Oxylipins are metabolites of polyunsaturated fatty acids that mediate cardiovascular health by attenuation of inflammation, vascular tone, hemostasis, and thrombosis.
33625776	4	91	theme	incorporation	807:819	arg1	rates					821:825	fatty acid incorporation rates	796:825	fatty acid incorporation rates	796:825	A compartmental model of deuterium-labeled linoleic acid and palmitic acid incorporation into VLDL was also developed to assess the dependence of VLDL oxylipins on fatty acid incorporation rates.
33625776	5	92	theme	%	1017:1017	arg1	%					1021:1021	29% [3%	1015:1021	29% [3%,49%]	1015:1026	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	5	92	theme	%	1017:1017	arg1	-EpODE					1007:1012	9(10)-EpODE	1002:1012	9(10)-EpODE	1002:1012	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	7	93	theme	[35	1371:1373	arg1	%					1369:1369	%	1369:1369	%	1369:1369	sEHi increased final concentration of DHA epoxide, 16(17)-EpDPE, by 99% [35%,193%], (2.0 nM).
33625776	5	94	dep	-1.3 nM	993:999	arg1	diols					1086:1090	AA-derived diols	1075:1090	AA-derived diols	1075:1090	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	5	94	dep	-1.3 nM	993:999	arg1	-EpODE					1007:1012	9(10)-EpODE	1002:1012	9(10)-EpODE	1002:1012	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	5	94	dep	-1.3 nM	993:999	arg1	%					1137:1137	31% [7%	1131:1137	31% [7%	1131:1137	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	5	94	dep	-1.3 nM	993:999	arg1	-1.0 nM					1145:1151	-1.0 nM	1145:1151	-1.0 nM	1145:1151	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	5	94	dep	-1.3 nM	993:999	arg1	-1.6 nM					1066:1072	-1.6 nM	1066:1072	-1.6 nM	1066:1072	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	5	94	dep	-1.3 nM	993:999	arg1	-DiHDPA					1122:1128	19(20)-DiHDPA	1116:1128	19(20)-DiHDPA	1116:1128	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	5	94	dep	-1.3 nM	993:999	arg1	%					1058:1058	29% [2%	1052:1058	29% [2%,49%]	1052:1063	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	5	94	dep	-1.3 nM	993:999	arg1	-EpODE					1044:1049	15(16)-EpODE	1038:1049	15(16)-EpODE	1038:1049	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	5	94	dep	-1.3 nM	993:999	arg1	%					1099:1099	32% [5%	1093:1099	32% [5%,52%]	1093:1104	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	5	94	dep	-1.3 nM	993:999	arg1	-2.4 nM					1107:1113	-2.4 nM	1107:1113	-2.4 nM	1107:1113	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	5	94	dep	-1.3 nM	993:999	arg1	%					1021:1021	29% [3%	1015:1021	29% [3%,49%]	1015:1026	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	5	94	dep	-1.3 nM	993:999	arg1	%					1141:1141	50%	1139:1141	50%	1139:1141	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	5	94	dep	-1.3 nM	993:999	arg1	-2.0 nM					1029:1035	-2.0 nM	1029:1035	-2.0 nM	1029:1035	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	7	95	theme	99	1367:1368	arg1	%					1369:1369	%	1369:1369	%	1369:1369	sEHi increased final concentration of DHA epoxide, 16(17)-EpDPE, by 99% [35%,193%], (2.0 nM).
33625776	0	96	from	livers	147:152	arg1	composition					77:87	oxylipin composition	68:87	oxylipin composition of very low-density lipoproteins in isolated perfused rat livers	68:152	Effects of inflammation and soluble epoxide hydrolase inhibition on oxylipin composition of very low-density lipoproteins in isolated perfused rat livers.
33625776	4	97	theme	oxylipins	783:791	arg1	dependence					764:773	the dependence	760:773	the dependence of VLDL oxylipins on fatty acid incorporation rates	760:825	A compartmental model of deuterium-labeled linoleic acid and palmitic acid incorporation into VLDL was also developed to assess the dependence of VLDL oxylipins on fatty acid incorporation rates.
33625776	5	98	theme	%	1095:1095	arg1	%					1099:1099	32% [5%	1093:1099	32% [5%,52%]	1093:1104	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	3	99	theme	liver	480:484	arg1	model					486:490	a perfused liver model	469:490	a perfused liver model	469:490	In this study, we used a perfused liver model to observe the effect of inflammatory lipopolysaccharide (LPS) challenge and soluble epoxide hydrolase inhibition (sEHi) on VLDL oxylipins.
33625776	5	100	theme	similar	953:959	arg1	amount					961:966	a similar amount	951:966	a similar amount (13-HOTrE, 35% [4%,55%]	951:990	LPS decreased the total fatty acid VLDL content by 30% [6%,47%], and decreased final concentration of several oxylipins by a similar amount (13-HOTrE, 35% [4%,55%], -1.3 nM; 9(10)-EpODE, 29% [3%,49%], -2.0 nM; 15(16)-EpODE, 29% [2%,49%], -1.6 nM; AA-derived diols, 32% [5%,52%], -2.4 nM; 19(20)-DiHDPA, 31% [7%,50%], -1.0 nM).
33625776	4	101	theme	fatty	796:800	arg1	rates					821:825	fatty acid incorporation rates	796:825	fatty acid incorporation rates	796:825	A compartmental model of deuterium-labeled linoleic acid and palmitic acid incorporation into VLDL was also developed to assess the dependence of VLDL oxylipins on fatty acid incorporation rates.
33625776	1	102	theme	inflammation	265:276	arg1	attenuation					250:260	attenuation	250:260	attenuation of inflammation, vascular tone, hemostasis, and thrombosis	250:319	Oxylipins are metabolites of polyunsaturated fatty acids that mediate cardiovascular health by attenuation of inflammation, vascular tone, hemostasis, and thrombosis.
34015094	4	0	theme	predicted	826:834	arg1	domains					846:852	no predicted enzymatic domains	823:852	no predicted enzymatic domains	823:852	Genomic sequencing was used to identify a mutant allele of ELMO1 which encodes a 20 kDa Golgi membrane protein that has no predicted enzymatic domains.
34015094	3	1	theme	plant	460:464	arg1	adhesion					471:478	plant cell adhesion	460:478	plant cell adhesion	460:478	To increase our understanding of plant cell adhesion, a population of ethyl methanesulfonate-mutagenized Arabidopsis were screened for hypocotyl adhesion defects using the pectin binding dye Ruthenium Red that penetrates defective but not wild-type (WT) hypocotyl cell walls.
34015094	4	2	used	used	726:729	arg2	sequencing					711:720	Genomic sequencing	703:720	Genomic sequencing	703:720	Genomic sequencing was used to identify a mutant allele of ELMO1 which encodes a 20 kDa Golgi membrane protein that has no predicted enzymatic domains.
34015094	1	3	theme	wall	239:242	arg1	structure					193:201	structure	193:201	structure	193:201	Plant growth, morphogenesis and development involve cellular adhesion, a process dependent on the composition and structure of the extracellular matrix or cell wall.
34015094	1	3	theme	wall	239:242	arg1	composition					177:187	composition	177:187	composition	177:187	Plant growth, morphogenesis and development involve cellular adhesion, a process dependent on the composition and structure of the extracellular matrix or cell wall.
34015094	3	4	theme	wild-type	666:674	arg1	walls					696:700	wild-type (WT) hypocotyl cell walls	666:700	hypocotyl adhesion defects using the pectin binding dye Ruthenium Red that penetrates defective but not wild-type (WT) hypocotyl cell walls	562:700	To increase our understanding of plant cell adhesion, a population of ethyl methanesulfonate-mutagenized Arabidopsis were screened for hypocotyl adhesion defects using the pectin binding dye Ruthenium Red that penetrates defective but not wild-type (WT) hypocotyl cell walls.
34015094	7	5	from	role	1196:1199	arg1	adhesion					1237:1244	plant cell adhesion	1226:1244	plant cell adhesion	1226:1244	elmo1 describes a previously unidentified role for the ELMO1 protein in plant cell adhesion.
34015094	3	6	theme	cell	466:469	arg1	adhesion					471:478	plant cell adhesion	460:478	plant cell adhesion	460:478	To increase our understanding of plant cell adhesion, a population of ethyl methanesulfonate-mutagenized Arabidopsis were screened for hypocotyl adhesion defects using the pectin binding dye Ruthenium Red that penetrates defective but not wild-type (WT) hypocotyl cell walls.
34015094	5	7	with	colocalizes	861:871	arg1	markers					892:898	several Golgi markers	878:898	several Golgi markers	878:898	ELMO1 colocalizes with several Golgi markers and elmo1-/- plants can be rescued by an ELMO1-GFP fusion.
34015094	5	7	with	colocalizes	861:871	arg1	plants					913:918	elmo1-/- plants	904:918	elmo1-/- plants	904:918	ELMO1 colocalizes with several Golgi markers and elmo1-/- plants can be rescued by an ELMO1-GFP fusion.
34015094	6	8	theme	other	1130:1134	arg1	mutants					1145:1151	other adhesion mutants	1130:1151	other adhesion mutants	1130:1151	elmo1-/- exhibits reduced mannose content relative to WT but no other cell wall changes and can be rescued to WT phenotype by mutants in ESMERALDA1, which also suppresses other adhesion mutants.
34015094	5	9	theme	elmo1-/-	904:911	arg1	plants					913:918	elmo1-/- plants	904:918	elmo1-/- plants	904:918	ELMO1 colocalizes with several Golgi markers and elmo1-/- plants can be rescued by an ELMO1-GFP fusion.
34015094	4	10	theme	enzymatic	836:844	arg1	domains					846:852	no predicted enzymatic domains	823:852	no predicted enzymatic domains	823:852	Genomic sequencing was used to identify a mutant allele of ELMO1 which encodes a 20 kDa Golgi membrane protein that has no predicted enzymatic domains.
34015094	3	11	theme	ethyl	497:501	arg1	Arabidopsis					532:542	ethyl methanesulfonate-mutagenized Arabidopsis	497:542	ethyl methanesulfonate-mutagenized Arabidopsis	497:542	To increase our understanding of plant cell adhesion, a population of ethyl methanesulfonate-mutagenized Arabidopsis were screened for hypocotyl adhesion defects using the pectin binding dye Ruthenium Red that penetrates defective but not wild-type (WT) hypocotyl cell walls.
34015094	1	12	theme	cellular	131:138	arg1	adhesion					140:147	cellular adhesion	131:147	cellular adhesion	131:147	Plant growth, morphogenesis and development involve cellular adhesion, a process dependent on the composition and structure of the extracellular matrix or cell wall.
34015094	1	12	theme	cellular	131:138	arg1	process					152:158	a process	150:158	a process dependent on the composition and structure of the extracellular matrix or cell wall	150:242	Plant growth, morphogenesis and development involve cellular adhesion, a process dependent on the composition and structure of the extracellular matrix or cell wall.
34015094	7	13	theme	plant	1226:1230	arg1	adhesion					1237:1244	plant cell adhesion	1226:1244	plant cell adhesion	1226:1244	elmo1 describes a previously unidentified role for the ELMO1 protein in plant cell adhesion.
34015094	2	14	theme	cell	259:262	arg1	wall					264:267	the cell wall	255:267	the cell wall	255:267	Pectin in the cell wall is thought to play an essential role in adhesion, and its modification and cleavage are suggested to be highly regulated so as to change adhesive properties.
34015094	1	15	theme	dependent	160:168	arg1	adhesion					140:147	cellular adhesion	131:147	cellular adhesion	131:147	Plant growth, morphogenesis and development involve cellular adhesion, a process dependent on the composition and structure of the extracellular matrix or cell wall.
34015094	1	15	theme	dependent	160:168	arg1	process					152:158	a process	150:158	a process dependent on the composition and structure of the extracellular matrix or cell wall	150:242	Plant growth, morphogenesis and development involve cellular adhesion, a process dependent on the composition and structure of the extracellular matrix or cell wall.
34015094	0	16	theme	protein	42:48	arg1	ELMO1					50:54	an Arabidopsis Golgi membrane protein ELMO1	12:54	an Arabidopsis Golgi membrane protein ELMO1	12:54	Mutation of an Arabidopsis Golgi membrane protein ELMO1 reduces cell adhesion.
34015094	3	17	theme	hypocotyl	681:689	arg1	walls					696:700	wild-type (WT) hypocotyl cell walls	666:700	hypocotyl adhesion defects using the pectin binding dye Ruthenium Red that penetrates defective but not wild-type (WT) hypocotyl cell walls	562:700	To increase our understanding of plant cell adhesion, a population of ethyl methanesulfonate-mutagenized Arabidopsis were screened for hypocotyl adhesion defects using the pectin binding dye Ruthenium Red that penetrates defective but not wild-type (WT) hypocotyl cell walls.
34015094	4	18	theme	membrane	797:804	arg1	allele					752:757	a mutant allele	743:757	a mutant allele of ELMO1 which encodes a 20 kDa Golgi membrane protein that has no predicted enzymatic domains	743:852	Genomic sequencing was used to identify a mutant allele of ELMO1 which encodes a 20 kDa Golgi membrane protein that has no predicted enzymatic domains.
34015094	4	18	theme	membrane	797:804	arg1	protein					806:812	a 20 kDa Golgi membrane protein	782:812	a 20 kDa Golgi membrane protein that has no predicted enzymatic domains	782:852	Genomic sequencing was used to identify a mutant allele of ELMO1 which encodes a 20 kDa Golgi membrane protein that has no predicted enzymatic domains.
34015094	6	19	dep	WT	1013:1014	arg1	changes					1039:1045	other cell wall changes	1023:1045	other cell wall changes	1023:1045	elmo1-/- exhibits reduced mannose content relative to WT but no other cell wall changes and can be rescued to WT phenotype by mutants in ESMERALDA1, which also suppresses other adhesion mutants.
34015094	5	20	theme	ELMO1-GFP	941:949	arg1	fusion					951:956	an ELMO1-GFP fusion	938:956	an ELMO1-GFP fusion	938:956	ELMO1 colocalizes with several Golgi markers and elmo1-/- plants can be rescued by an ELMO1-GFP fusion.
34015094	6	21	theme	wall	1034:1037	arg1	changes					1039:1045	other cell wall changes	1023:1045	other cell wall changes	1023:1045	elmo1-/- exhibits reduced mannose content relative to WT but no other cell wall changes and can be rescued to WT phenotype by mutants in ESMERALDA1, which also suppresses other adhesion mutants.
34015094	3	22	theme	cell	691:694	arg1	walls					696:700	wild-type (WT) hypocotyl cell walls	666:700	hypocotyl adhesion defects using the pectin binding dye Ruthenium Red that penetrates defective but not wild-type (WT) hypocotyl cell walls	562:700	To increase our understanding of plant cell adhesion, a population of ethyl methanesulfonate-mutagenized Arabidopsis were screened for hypocotyl adhesion defects using the pectin binding dye Ruthenium Red that penetrates defective but not wild-type (WT) hypocotyl cell walls.
34015094	7	23	theme	ELMO1	1209:1213	arg1	protein					1215:1221	the ELMO1 protein	1205:1221	the ELMO1 protein	1205:1221	elmo1 describes a previously unidentified role for the ELMO1 protein in plant cell adhesion.
34015094	1	24	theme	Plant	79:83	arg1	growth					85:90	Plant growth	79:90	Plant growth	79:90	Plant growth, morphogenesis and development involve cellular adhesion, a process dependent on the composition and structure of the extracellular matrix or cell wall.
34015094	6	25	theme	cell	1029:1032	arg1	changes					1039:1045	other cell wall changes	1023:1045	other cell wall changes	1023:1045	elmo1-/- exhibits reduced mannose content relative to WT but no other cell wall changes and can be rescued to WT phenotype by mutants in ESMERALDA1, which also suppresses other adhesion mutants.
34015094	6	26	theme	relative	1001:1008	arg1	content					993:999	reduced mannose content	977:999	reduced mannose content relative to WT but no other cell wall changes	977:1045	elmo1-/- exhibits reduced mannose content relative to WT but no other cell wall changes and can be rescued to WT phenotype by mutants in ESMERALDA1, which also suppresses other adhesion mutants.
34015094	3	27	dep	wild-type	666:674	arg1	WT					677:678	WT	677:678	WT	677:678	To increase our understanding of plant cell adhesion, a population of ethyl methanesulfonate-mutagenized Arabidopsis were screened for hypocotyl adhesion defects using the pectin binding dye Ruthenium Red that penetrates defective but not wild-type (WT) hypocotyl cell walls.
34015094	6	28	theme	reduced	977:983	arg1	content					993:999	reduced mannose content	977:999	reduced mannose content relative to WT but no other cell wall changes	977:1045	elmo1-/- exhibits reduced mannose content relative to WT but no other cell wall changes and can be rescued to WT phenotype by mutants in ESMERALDA1, which also suppresses other adhesion mutants.
34015094	4	29	theme	Genomic	703:709	arg1	sequencing					711:720	Genomic sequencing	703:720	Genomic sequencing	703:720	Genomic sequencing was used to identify a mutant allele of ELMO1 which encodes a 20 kDa Golgi membrane protein that has no predicted enzymatic domains.
34015094	6	30	theme	other	1023:1027	arg1	changes					1039:1045	other cell wall changes	1023:1045	other cell wall changes	1023:1045	elmo1-/- exhibits reduced mannose content relative to WT but no other cell wall changes and can be rescued to WT phenotype by mutants in ESMERALDA1, which also suppresses other adhesion mutants.
34015094	0	31	theme	Arabidopsis	15:25	arg1	ELMO1					50:54	an Arabidopsis Golgi membrane protein ELMO1	12:54	an Arabidopsis Golgi membrane protein ELMO1	12:54	Mutation of an Arabidopsis Golgi membrane protein ELMO1 reduces cell adhesion.
34015094	4	32	theme	20 kDa	784:789	arg1	allele					752:757	a mutant allele	743:757	a mutant allele of ELMO1 which encodes a 20 kDa Golgi membrane protein that has no predicted enzymatic domains	743:852	Genomic sequencing was used to identify a mutant allele of ELMO1 which encodes a 20 kDa Golgi membrane protein that has no predicted enzymatic domains.
34015094	4	32	theme	20 kDa	784:789	arg1	protein					806:812	a 20 kDa Golgi membrane protein	782:812	a 20 kDa Golgi membrane protein that has no predicted enzymatic domains	782:852	Genomic sequencing was used to identify a mutant allele of ELMO1 which encodes a 20 kDa Golgi membrane protein that has no predicted enzymatic domains.
34015094	3	33	theme	methanesulfonate-mutagenized	503:530	arg1	Arabidopsis					532:542	ethyl methanesulfonate-mutagenized Arabidopsis	497:542	ethyl methanesulfonate-mutagenized Arabidopsis	497:542	To increase our understanding of plant cell adhesion, a population of ethyl methanesulfonate-mutagenized Arabidopsis were screened for hypocotyl adhesion defects using the pectin binding dye Ruthenium Red that penetrates defective but not wild-type (WT) hypocotyl cell walls.
34015094	6	34	from	mutants	1085:1091	arg1	ESMERALDA1					1096:1105	ESMERALDA1	1096:1105	ESMERALDA1	1096:1105	elmo1-/- exhibits reduced mannose content relative to WT but no other cell wall changes and can be rescued to WT phenotype by mutants in ESMERALDA1, which also suppresses other adhesion mutants.
34015094	4	35	theme	mutant	745:750	arg1	allele					752:757	a mutant allele	743:757	a mutant allele of ELMO1 which encodes a 20 kDa Golgi membrane protein that has no predicted enzymatic domains	743:852	Genomic sequencing was used to identify a mutant allele of ELMO1 which encodes a 20 kDa Golgi membrane protein that has no predicted enzymatic domains.
34015094	4	35	theme	mutant	745:750	arg1	protein					806:812	a 20 kDa Golgi membrane protein	782:812	a 20 kDa Golgi membrane protein that has no predicted enzymatic domains	782:852	Genomic sequencing was used to identify a mutant allele of ELMO1 which encodes a 20 kDa Golgi membrane protein that has no predicted enzymatic domains.
34015094	3	36	theme	Red	628:630	arg1	Ruthenium					618:626	the pectin binding dye Ruthenium Red	595:630	the pectin binding dye Ruthenium Red that penetrates defective	595:656	To increase our understanding of plant cell adhesion, a population of ethyl methanesulfonate-mutagenized Arabidopsis were screened for hypocotyl adhesion defects using the pectin binding dye Ruthenium Red that penetrates defective but not wild-type (WT) hypocotyl cell walls.
34015094	3	37	theme	Arabidopsis	532:542	arg1	population					483:492	a population	481:492	a population of ethyl methanesulfonate-mutagenized Arabidopsis	481:542	To increase our understanding of plant cell adhesion, a population of ethyl methanesulfonate-mutagenized Arabidopsis were screened for hypocotyl adhesion defects using the pectin binding dye Ruthenium Red that penetrates defective but not wild-type (WT) hypocotyl cell walls.
34015094	0	38	theme	membrane	33:40	arg1	ELMO1					50:54	an Arabidopsis Golgi membrane protein ELMO1	12:54	an Arabidopsis Golgi membrane protein ELMO1	12:54	Mutation of an Arabidopsis Golgi membrane protein ELMO1 reduces cell adhesion.
34015094	7	39	theme	cell	1232:1235	arg1	adhesion					1237:1244	plant cell adhesion	1226:1244	plant cell adhesion	1226:1244	elmo1 describes a previously unidentified role for the ELMO1 protein in plant cell adhesion.
34015094	3	40	theme	adhesion	572:579	arg1	defects					581:587	hypocotyl adhesion defects	562:587	hypocotyl adhesion defects using the pectin binding dye Ruthenium Red that penetrates defective but not wild-type (WT) hypocotyl cell walls	562:700	To increase our understanding of plant cell adhesion, a population of ethyl methanesulfonate-mutagenized Arabidopsis were screened for hypocotyl adhesion defects using the pectin binding dye Ruthenium Red that penetrates defective but not wild-type (WT) hypocotyl cell walls.
34015094	2	41	from	Pectin	245:250	arg1	wall					264:267	the cell wall	255:267	the cell wall	255:267	Pectin in the cell wall is thought to play an essential role in adhesion, and its modification and cleavage are suggested to be highly regulated so as to change adhesive properties.
34015094	0	42	theme	Golgi	27:31	arg1	ELMO1					50:54	an Arabidopsis Golgi membrane protein ELMO1	12:54	an Arabidopsis Golgi membrane protein ELMO1	12:54	Mutation of an Arabidopsis Golgi membrane protein ELMO1 reduces cell adhesion.
34015094	3	43	theme	dye	614:616	arg1	Ruthenium					618:626	the pectin binding dye Ruthenium Red	595:630	the pectin binding dye Ruthenium Red that penetrates defective	595:656	To increase our understanding of plant cell adhesion, a population of ethyl methanesulfonate-mutagenized Arabidopsis were screened for hypocotyl adhesion defects using the pectin binding dye Ruthenium Red that penetrates defective but not wild-type (WT) hypocotyl cell walls.
34015094	1	44	dep	composition	177:187	arg1	the					173:175	the	173:175	the	173:175	Plant growth, morphogenesis and development involve cellular adhesion, a process dependent on the composition and structure of the extracellular matrix or cell wall.
34015094	0	45	theme	ELMO1	50:54	arg1	Mutation					0:7	Mutation	0:7	Mutation of an Arabidopsis Golgi membrane protein ELMO1	0:54	Mutation of an Arabidopsis Golgi membrane protein ELMO1 reduces cell adhesion.
34015094	4	46	theme	Golgi	791:795	arg1	allele					752:757	a mutant allele	743:757	a mutant allele of ELMO1 which encodes a 20 kDa Golgi membrane protein that has no predicted enzymatic domains	743:852	Genomic sequencing was used to identify a mutant allele of ELMO1 which encodes a 20 kDa Golgi membrane protein that has no predicted enzymatic domains.
34015094	4	46	theme	Golgi	791:795	arg1	protein					806:812	a 20 kDa Golgi membrane protein	782:812	a 20 kDa Golgi membrane protein that has no predicted enzymatic domains	782:852	Genomic sequencing was used to identify a mutant allele of ELMO1 which encodes a 20 kDa Golgi membrane protein that has no predicted enzymatic domains.
34015094	5	47	theme	several	878:884	arg1	markers					892:898	several Golgi markers	878:898	several Golgi markers	878:898	ELMO1 colocalizes with several Golgi markers and elmo1-/- plants can be rescued by an ELMO1-GFP fusion.
34015094	2	48	theme	adhesive	406:413	arg1	properties					415:424	adhesive properties	406:424	adhesive properties	406:424	Pectin in the cell wall is thought to play an essential role in adhesion, and its modification and cleavage are suggested to be highly regulated so as to change adhesive properties.
34015094	3	49	theme	hypocotyl	562:570	arg1	defects					581:587	hypocotyl adhesion defects	562:587	hypocotyl adhesion defects using the pectin binding dye Ruthenium Red that penetrates defective but not wild-type (WT) hypocotyl cell walls	562:700	To increase our understanding of plant cell adhesion, a population of ethyl methanesulfonate-mutagenized Arabidopsis were screened for hypocotyl adhesion defects using the pectin binding dye Ruthenium Red that penetrates defective but not wild-type (WT) hypocotyl cell walls.
34015094	5	50	theme	Golgi	886:890	arg1	markers					892:898	several Golgi markers	878:898	several Golgi markers	878:898	ELMO1 colocalizes with several Golgi markers and elmo1-/- plants can be rescued by an ELMO1-GFP fusion.
34015094	6	51	theme	adhesion	1136:1143	arg1	mutants					1145:1151	other adhesion mutants	1130:1151	other adhesion mutants	1130:1151	elmo1-/- exhibits reduced mannose content relative to WT but no other cell wall changes and can be rescued to WT phenotype by mutants in ESMERALDA1, which also suppresses other adhesion mutants.
34015094	3	52	theme	pectin	599:604	arg1	Ruthenium					618:626	the pectin binding dye Ruthenium Red	595:630	the pectin binding dye Ruthenium Red that penetrates defective	595:656	To increase our understanding of plant cell adhesion, a population of ethyl methanesulfonate-mutagenized Arabidopsis were screened for hypocotyl adhesion defects using the pectin binding dye Ruthenium Red that penetrates defective but not wild-type (WT) hypocotyl cell walls.
34015094	6	53	theme	WT	1069:1070	arg1	phenotype					1072:1080	WT phenotype	1069:1080	WT phenotype	1069:1080	elmo1-/- exhibits reduced mannose content relative to WT but no other cell wall changes and can be rescued to WT phenotype by mutants in ESMERALDA1, which also suppresses other adhesion mutants.
34015094	4	54	contain	has	819:821	arg2	domains					846:852	no predicted enzymatic domains	823:852	no predicted enzymatic domains	823:852	Genomic sequencing was used to identify a mutant allele of ELMO1 which encodes a 20 kDa Golgi membrane protein that has no predicted enzymatic domains.
34015094	4	54	contain	has	819:821	arg1	allele					752:757	a mutant allele	743:757	a mutant allele of ELMO1 which encodes a 20 kDa Golgi membrane protein that has no predicted enzymatic domains	743:852	Genomic sequencing was used to identify a mutant allele of ELMO1 which encodes a 20 kDa Golgi membrane protein that has no predicted enzymatic domains.
34015094	4	54	contain	has	819:821	arg1	protein					806:812	a 20 kDa Golgi membrane protein	782:812	a 20 kDa Golgi membrane protein that has no predicted enzymatic domains	782:852	Genomic sequencing was used to identify a mutant allele of ELMO1 which encodes a 20 kDa Golgi membrane protein that has no predicted enzymatic domains.
34015094	1	55	theme	extracellular	210:222	arg1	matrix					224:229	the extracellular matrix	206:229	the extracellular matrix	206:229	Plant growth, morphogenesis and development involve cellular adhesion, a process dependent on the composition and structure of the extracellular matrix or cell wall.
34015094	3	56	theme	binding	606:612	arg1	Ruthenium					618:626	the pectin binding dye Ruthenium Red	595:630	the pectin binding dye Ruthenium Red that penetrates defective	595:656	To increase our understanding of plant cell adhesion, a population of ethyl methanesulfonate-mutagenized Arabidopsis were screened for hypocotyl adhesion defects using the pectin binding dye Ruthenium Red that penetrates defective but not wild-type (WT) hypocotyl cell walls.
34015094	2	57	theme	essential	291:299	arg1	role					301:304	an essential role	288:304	an essential role	288:304	Pectin in the cell wall is thought to play an essential role in adhesion, and its modification and cleavage are suggested to be highly regulated so as to change adhesive properties.
34015094	1	58	theme	matrix	224:229	arg1	structure					193:201	structure	193:201	structure	193:201	Plant growth, morphogenesis and development involve cellular adhesion, a process dependent on the composition and structure of the extracellular matrix or cell wall.
34015094	1	58	theme	matrix	224:229	arg1	composition					177:187	composition	177:187	composition	177:187	Plant growth, morphogenesis and development involve cellular adhesion, a process dependent on the composition and structure of the extracellular matrix or cell wall.
34015094	0	59	theme	cell	64:67	arg1	adhesion					69:76	cell adhesion	64:76	cell adhesion	64:76	Mutation of an Arabidopsis Golgi membrane protein ELMO1 reduces cell adhesion.
34015094	3	60	theme	adhesion	471:478	arg1	understanding					443:455	our understanding	439:455	our understanding of plant cell adhesion	439:478	To increase our understanding of plant cell adhesion, a population of ethyl methanesulfonate-mutagenized Arabidopsis were screened for hypocotyl adhesion defects using the pectin binding dye Ruthenium Red that penetrates defective but not wild-type (WT) hypocotyl cell walls.
34015094	6	61	theme	mannose	985:991	arg1	content					993:999	reduced mannose content	977:999	reduced mannose content relative to WT but no other cell wall changes	977:1045	elmo1-/- exhibits reduced mannose content relative to WT but no other cell wall changes and can be rescued to WT phenotype by mutants in ESMERALDA1, which also suppresses other adhesion mutants.
34015094	7	62	theme	unidentified	1183:1194	arg1	role					1196:1199	a previously unidentified role	1170:1199	a previously unidentified role for the ELMO1 protein in plant cell adhesion	1170:1244	elmo1 describes a previously unidentified role for the ELMO1 protein in plant cell adhesion.
34015094	4	63	theme	ELMO1	762:766	arg1	allele					752:757	a mutant allele	743:757	a mutant allele of ELMO1 which encodes a 20 kDa Golgi membrane protein that has no predicted enzymatic domains	743:852	Genomic sequencing was used to identify a mutant allele of ELMO1 which encodes a 20 kDa Golgi membrane protein that has no predicted enzymatic domains.
34015094	4	63	theme	ELMO1	762:766	arg1	protein					806:812	a 20 kDa Golgi membrane protein	782:812	a 20 kDa Golgi membrane protein that has no predicted enzymatic domains	782:852	Genomic sequencing was used to identify a mutant allele of ELMO1 which encodes a 20 kDa Golgi membrane protein that has no predicted enzymatic domains.
34015094	5	64	theme	ELMO1	855:859	arg1	colocalizes					861:871	ELMO1 colocalizes	855:871	ELMO1 colocalizes with several Golgi markers and elmo1-/- plants	855:918	ELMO1 colocalizes with several Golgi markers and elmo1-/- plants can be rescued by an ELMO1-GFP fusion.
34015094	1	65	theme	cell	234:237	arg1	wall					239:242	cell wall	234:242	cell wall	234:242	Plant growth, morphogenesis and development involve cellular adhesion, a process dependent on the composition and structure of the extracellular matrix or cell wall.
32726162	9	0	from	deficits	2019:2026	arg1	P56					2042:2044	P56	2042:2044	P56	2042:2044	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	9	0	from	deficits	2019:2026	arg1	adulthood					2031:2039	adulthood	2031:2039	adulthood (P56)	2031:2045	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	2	1	theme	active	365:370	arg1	disease					372:378	active disease	365:378	active disease	365:378	These neuropsychiatric effects include anxiety, depression, and memory impairments that can be seen both during active disease and following remission and are more frequently seen in pediatric patients.
32726162	6	2	theme	object	1088:1093	arg1	task					1107:1110	novel object recognition task	1082:1110	novel object recognition task	1082:1110	Despite resolution of acute disease, cognitive deficits (novel object recognition task) and anxiety-like behavior (light/dark box) were identified in the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning.
32726162	6	2	theme	object	1088:1093	arg1	deficits					1072:1079	cognitive deficits	1062:1079	cognitive deficits (novel object recognition task)	1062:1111	Despite resolution of acute disease, cognitive deficits (novel object recognition task) and anxiety-like behavior (light/dark box) were identified in the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning.
32726162	1	3	theme	bowel	124:128	arg1	diseases					169:176	chronic intestinal diseases	150:176	chronic intestinal diseases	150:176	Inflammatory bowel diseases (IBDs) are chronic intestinal diseases, frequently associated with comorbid psychological and cognitive deficits.
32726162	1	3	theme	bowel	124:128	arg1	IBDs					140:143	IBDs	140:143	IBDs	140:143	Inflammatory bowel diseases (IBDs) are chronic intestinal diseases, frequently associated with comorbid psychological and cognitive deficits.
32726162	1	3	theme	bowel	124:128	arg1	diseases					130:137	Inflammatory bowel diseases	111:137	Inflammatory bowel diseases (IBDs)	111:144	Inflammatory bowel diseases (IBDs) are chronic intestinal diseases, frequently associated with comorbid psychological and cognitive deficits.
32726162	8	4	from	alterations	1492:1502	arg1	composition					1526:1536	the gut microbiota composition	1507:1536	the gut microbiota composition	1507:1536	Additionally, persistent alterations in the gut microbiota composition were observed at P56, including reduced butyrate-producing species.
32726162	8	5	located	observed	1543:1550	arg2	alterations					1492:1502	persistent alterations	1481:1502	persistent alterations in the gut microbiota composition	1481:1536	Additionally, persistent alterations in the gut microbiota composition were observed at P56, including reduced butyrate-producing species.
32726162	8	5	located	observed	1543:1550	arg1	P56					1555:1557	P56	1555:1557	P56	1555:1557	Additionally, persistent alterations in the gut microbiota composition were observed at P56, including reduced butyrate-producing species.
32726162	6	6	theme	cognitive	1062:1070	arg1	task					1107:1110	novel object recognition task	1082:1110	novel object recognition task	1082:1110	Despite resolution of acute disease, cognitive deficits (novel object recognition task) and anxiety-like behavior (light/dark box) were identified in the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning.
32726162	6	6	theme	cognitive	1062:1070	arg1	deficits					1072:1079	cognitive deficits	1062:1079	cognitive deficits (novel object recognition task)	1062:1111	Despite resolution of acute disease, cognitive deficits (novel object recognition task) and anxiety-like behavior (light/dark box) were identified in the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning.
32726162	7	7	from	neuroinflammation	1343:1359	arg1	hippocampus					1454:1464	the hippocampus	1450:1464	the hippocampus	1450:1464	Behavioral deficits were found in conjunction with neuroinflammation, decreased neurogenesis, and altered expression of pattern recognition receptor genes in the hippocampus.
32726162	6	8	theme	disease	1053:1059	arg1	resolution					1033:1042	resolution	1033:1042	resolution of acute disease	1033:1059	Despite resolution of acute disease, cognitive deficits (novel object recognition task) and anxiety-like behavior (light/dark box) were identified in the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning.
32726162	6	9	theme	field	1228:1232	arg1	behavior					1213:1220	exploratory behavior	1201:1220	exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning	1201:1289	Despite resolution of acute disease, cognitive deficits (novel object recognition task) and anxiety-like behavior (light/dark box) were identified in the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning.
32726162	6	9	theme	field	1228:1232	arg1	test					1234:1237	open field test	1223:1237	open field test	1223:1237	Despite resolution of acute disease, cognitive deficits (novel object recognition task) and anxiety-like behavior (light/dark box) were identified in the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning.
32726162	3	10	theme	phenomenon	565:574	arg1	study					550:554	the study	546:554	the study of these phenomenon	546:574	The mechanism(s) through which these extraintestinal deficits develop remain unknown, and the study of these phenomenon is hampered by a lack of murine pediatric IBD models.
32726162	1	11	theme	comorbid	206:213	arg1	deficits					243:250	comorbid psychological and cognitive deficits	206:250	comorbid psychological and cognitive deficits	206:250	Inflammatory bowel diseases (IBDs) are chronic intestinal diseases, frequently associated with comorbid psychological and cognitive deficits.
32726162	6	12	theme	light/dark	1140:1149	arg1	behavior					1130:1137	anxiety-like behavior	1117:1137	anxiety-like behavior (light/dark box)	1117:1154	Despite resolution of acute disease, cognitive deficits (novel object recognition task) and anxiety-like behavior (light/dark box) were identified in the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning.
32726162	6	12	theme	light/dark	1140:1149	arg1	box					1151:1153	light/dark box	1140:1153	light/dark box	1140:1153	Despite resolution of acute disease, cognitive deficits (novel object recognition task) and anxiety-like behavior (light/dark box) were identified in the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning.
32726162	4	13	theme	colitis	713:719	arg1	induction					700:708	induction	700:708	induction of colitis in a pediatric setting	700:742	Herein we describe microbiota-gut-brain (MGB) axis deficits following induction of colitis in a pediatric setting.
32726162	6	14	theme	exploratory	1201:1211	arg1	behavior					1213:1220	exploratory behavior	1201:1220	exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning	1201:1289	Despite resolution of acute disease, cognitive deficits (novel object recognition task) and anxiety-like behavior (light/dark box) were identified in the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning.
32726162	6	14	theme	exploratory	1201:1211	arg1	test					1234:1237	open field test	1223:1237	open field test	1223:1237	Despite resolution of acute disease, cognitive deficits (novel object recognition task) and anxiety-like behavior (light/dark box) were identified in the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning.
32726162	9	15	theme	first	1653:1657	arg1	time					1659:1662	the first time	1649:1662	the first time	1649:1662	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	9	16	theme	dextran	1899:1905	arg1	sulfate					1914:1920	low-dose dextran sodium sulfate	1890:1920	low-dose dextran sodium sulfate (DSS)	1890:1926	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	9	16	theme	dextran	1899:1905	arg1	DSS					1923:1925	DSS	1923:1925	DSS	1923:1925	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	6	17	theme	changes	1190:1196	arg1	absence					1179:1185	the absence	1175:1185	the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning	1175:1289	Despite resolution of acute disease, cognitive deficits (novel object recognition task) and anxiety-like behavior (light/dark box) were identified in the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning.
32726162	3	18	theme	models	622:627	arg1	lack					593:596	a lack	591:596	a lack of murine pediatric IBD models	591:627	The mechanism(s) through which these extraintestinal deficits develop remain unknown, and the study of these phenomenon is hampered by a lack of murine pediatric IBD models.
32726162	9	19	theme	sulfate	1914:1920	arg1	administration					1872:1885	administration	1872:1885	administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56)	1872:2045	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	3	20	theme	pediatric	608:616	arg1	models					622:627	murine pediatric IBD models	601:627	murine pediatric IBD models	601:627	The mechanism(s) through which these extraintestinal deficits develop remain unknown, and the study of these phenomenon is hampered by a lack of murine pediatric IBD models.
32726162	11	21	theme	disease	2335:2341	arg1	features					2294:2301	the unique features	2283:2301	the unique features of pediatric inflammatory bowel disease	2283:2341	This model highlights the unique features of pediatric inflammatory bowel disease.
32726162	10	22	theme	acute	2072:2076	arg1	inflammation					2086:2097	acute colonic inflammation	2072:2097	acute colonic inflammation	2072:2097	Early-life DSS leads to acute colonic inflammation, similar to adult mice; however, it results in long-lasting deficits in the MGB axis in adulthood (P56), in contrast to the transient deficits seen in adult DSS.
32726162	0	23	theme	axis	83:86	arg1	deficits					88:95	microbiota-gut-brain axis deficits	62:95	microbiota-gut-brain axis deficits	62:95	A murine model of pediatric inflammatory bowel disease causes microbiota-gut-brain axis deficits in adulthood.
32726162	1	24	theme	intestinal	158:167	arg1	diseases					169:176	chronic intestinal diseases	150:176	chronic intestinal diseases	150:176	Inflammatory bowel diseases (IBDs) are chronic intestinal diseases, frequently associated with comorbid psychological and cognitive deficits.
32726162	1	24	theme	intestinal	158:167	arg1	diseases					130:137	Inflammatory bowel diseases	111:137	Inflammatory bowel diseases (IBDs)	111:144	Inflammatory bowel diseases (IBDs) are chronic intestinal diseases, frequently associated with comorbid psychological and cognitive deficits.
32726162	9	25	theme	axis	1684:1687	arg1	deficits					1689:1696	MGB axis deficits	1680:1696	MGB axis deficits	1680:1696	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	11	26	theme	inflammatory	2316:2327	arg1	disease					2335:2341	pediatric inflammatory bowel disease	2306:2341	pediatric inflammatory bowel disease	2306:2341	This model highlights the unique features of pediatric inflammatory bowel disease.
32726162	10	27	theme	adult	2111:2115	arg1	mice					2117:2120	adult mice	2111:2120	adult mice	2111:2120	Early-life DSS leads to acute colonic inflammation, similar to adult mice; however, it results in long-lasting deficits in the MGB axis in adulthood (P56), in contrast to the transient deficits seen in adult DSS.
32726162	6	28	theme	P56	1243:1245	arg1	mice					1247:1250	P56 mice	1243:1250	P56 mice previously treated with DSS at weaning	1243:1289	Despite resolution of acute disease, cognitive deficits (novel object recognition task) and anxiety-like behavior (light/dark box) were identified in the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning.
32726162	9	29	from	impacts	1815:1821	arg1	axis					1857:1860	the microbiota-gut-brain (MGB) axis	1826:1860	the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56)	1826:2045	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	9	29	from	impacts	1815:1821	arg1	MGB					1852:1854	MGB	1852:1854	MGB	1852:1854	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	10	30	theme	similar	2100:2106	arg1	inflammation					2086:2097	acute colonic inflammation	2072:2097	acute colonic inflammation	2072:2097	Early-life DSS leads to acute colonic inflammation, similar to adult mice; however, it results in long-lasting deficits in the MGB axis in adulthood (P56), in contrast to the transient deficits seen in adult DSS.
32726162	5	31	theme	Acute	745:749	arg1	colitis					751:757	Acute colitis	745:757	Acute colitis	745:757	Acute colitis was induced by administration of 2% dextran sodium sulfate (DSS) for 5 days starting at weaning [postnatal day (P)21] causing reduced weight gain, colonic shortening, and colonic inflammation by 8 days post-DSS (P29), which were mostly resolved in adult (P56) mice.
32726162	9	32	from	weaning	1732:1738	arg1	induction					1708:1716	induction	1708:1716	induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56)	1708:2045	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	5	33	theme	sodium	803:808	arg1	DSS					819:821	DSS	819:821	DSS	819:821	Acute colitis was induced by administration of 2% dextran sodium sulfate (DSS) for 5 days starting at weaning [postnatal day (P)21] causing reduced weight gain, colonic shortening, and colonic inflammation by 8 days post-DSS (P29), which were mostly resolved in adult (P56) mice.
32726162	5	33	theme	sodium	803:808	arg1	sulfate					810:816	2% dextran sodium sulfate	792:816	2% dextran sodium sulfate (DSS)	792:822	Acute colitis was induced by administration of 2% dextran sodium sulfate (DSS) for 5 days starting at weaning [postnatal day (P)21] causing reduced weight gain, colonic shortening, and colonic inflammation by 8 days post-DSS (P29), which were mostly resolved in adult (P56) mice.
32726162	5	34	theme	weight	893:898	arg1	gain					900:903	reduced weight gain	885:903	reduced weight gain	885:903	Acute colitis was induced by administration of 2% dextran sodium sulfate (DSS) for 5 days starting at weaning [postnatal day (P)21] causing reduced weight gain, colonic shortening, and colonic inflammation by 8 days post-DSS (P29), which were mostly resolved in adult (P56) mice.
32726162	5	35	theme	2	792:792	arg1	%					793:793	%	793:793	%	793:793	Acute colitis was induced by administration of 2% dextran sodium sulfate (DSS) for 5 days starting at weaning [postnatal day (P)21] causing reduced weight gain, colonic shortening, and colonic inflammation by 8 days post-DSS (P29), which were mostly resolved in adult (P56) mice.
32726162	2	36	theme	pediatric	436:444	arg1	patients					446:453	pediatric patients	436:453	pediatric patients	436:453	These neuropsychiatric effects include anxiety, depression, and memory impairments that can be seen both during active disease and following remission and are more frequently seen in pediatric patients.
32726162	7	37	theme	genes	1441:1445	arg1	neuroinflammation					1343:1359	neuroinflammation	1343:1359	neuroinflammation	1343:1359	Behavioral deficits were found in conjunction with neuroinflammation, decreased neurogenesis, and altered expression of pattern recognition receptor genes in the hippocampus.
32726162	7	37	theme	genes	1441:1445	arg1	neurogenesis					1372:1383	decreased neurogenesis	1362:1383	decreased neurogenesis	1362:1383	Behavioral deficits were found in conjunction with neuroinflammation, decreased neurogenesis, and altered expression of pattern recognition receptor genes in the hippocampus.
32726162	7	37	theme	genes	1441:1445	arg1	expression					1398:1407	altered expression	1390:1407	altered expression of pattern recognition receptor genes in the hippocampus	1390:1464	Behavioral deficits were found in conjunction with neuroinflammation, decreased neurogenesis, and altered expression of pattern recognition receptor genes in the hippocampus.
32726162	1	38	theme	Inflammatory	111:122	arg1	diseases					169:176	chronic intestinal diseases	150:176	chronic intestinal diseases	150:176	Inflammatory bowel diseases (IBDs) are chronic intestinal diseases, frequently associated with comorbid psychological and cognitive deficits.
32726162	1	38	theme	Inflammatory	111:122	arg1	IBDs					140:143	IBDs	140:143	IBDs	140:143	Inflammatory bowel diseases (IBDs) are chronic intestinal diseases, frequently associated with comorbid psychological and cognitive deficits.
32726162	1	38	theme	Inflammatory	111:122	arg1	diseases					130:137	Inflammatory bowel diseases	111:137	Inflammatory bowel diseases (IBDs)	111:144	Inflammatory bowel diseases (IBDs) are chronic intestinal diseases, frequently associated with comorbid psychological and cognitive deficits.
32726162	9	39	theme	gut	1961:1963	arg1	dysbiosis					1965:1973	gut dysbiosis	1961:1973	gut dysbiosis	1961:1973	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	0	40	theme	pediatric	18:26	arg1	disease					47:53	pediatric inflammatory bowel disease	18:53	pediatric inflammatory bowel disease	18:53	A murine model of pediatric inflammatory bowel disease causes microbiota-gut-brain axis deficits in adulthood.
32726162	7	41	theme	recognition	1420:1430	arg1	receptor					1432:1439	pattern recognition receptor	1412:1439	pattern recognition receptor genes	1412:1445	Behavioral deficits were found in conjunction with neuroinflammation, decreased neurogenesis, and altered expression of pattern recognition receptor genes in the hippocampus.
32726162	3	42	theme	extraintestinal	493:507	arg1	deficits					509:516	these extraintestinal deficits	487:516	these extraintestinal deficits	487:516	The mechanism(s) through which these extraintestinal deficits develop remain unknown, and the study of these phenomenon is hampered by a lack of murine pediatric IBD models.
32726162	5	43	theme	%	793:793	arg1	DSS					819:821	DSS	819:821	DSS	819:821	Acute colitis was induced by administration of 2% dextran sodium sulfate (DSS) for 5 days starting at weaning [postnatal day (P)21] causing reduced weight gain, colonic shortening, and colonic inflammation by 8 days post-DSS (P29), which were mostly resolved in adult (P56) mice.
32726162	5	43	theme	%	793:793	arg1	sulfate					810:816	2% dextran sodium sulfate	792:816	2% dextran sodium sulfate (DSS)	792:822	Acute colitis was induced by administration of 2% dextran sodium sulfate (DSS) for 5 days starting at weaning [postnatal day (P)21] causing reduced weight gain, colonic shortening, and colonic inflammation by 8 days post-DSS (P29), which were mostly resolved in adult (P56) mice.
32726162	6	44	from	behavior	1213:1220	arg1	mice					1247:1250	P56 mice	1243:1250	P56 mice previously treated with DSS at weaning	1243:1289	Despite resolution of acute disease, cognitive deficits (novel object recognition task) and anxiety-like behavior (light/dark box) were identified in the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning.
32726162	6	44	from	behavior	1213:1220	arg1	absence					1179:1185	the absence	1175:1185	the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning	1175:1289	Despite resolution of acute disease, cognitive deficits (novel object recognition task) and anxiety-like behavior (light/dark box) were identified in the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning.
32726162	9	45	theme	adulthood.NEW	1758:1770	arg1	&					1772:1772	adulthood.NEW &	1758:1772	adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56)	1758:2045	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	2	46	theme	neuropsychiatric	259:274	arg1	effects					276:282	These neuropsychiatric effects	253:282	These neuropsychiatric effects	253:282	These neuropsychiatric effects include anxiety, depression, and memory impairments that can be seen both during active disease and following remission and are more frequently seen in pediatric patients.
32726162	1	47	theme	cognitive	233:241	arg1	deficits					243:250	comorbid psychological and cognitive deficits	206:250	comorbid psychological and cognitive deficits	206:250	Inflammatory bowel diseases (IBDs) are chronic intestinal diseases, frequently associated with comorbid psychological and cognitive deficits.
32726162	7	48	theme	altered	1390:1396	arg1	expression					1398:1407	altered expression	1390:1407	altered expression of pattern recognition receptor genes in the hippocampus	1390:1464	Behavioral deficits were found in conjunction with neuroinflammation, decreased neurogenesis, and altered expression of pattern recognition receptor genes in the hippocampus.
32726162	8	49	theme	reduced	1570:1576	arg1	species					1597:1603	reduced butyrate-producing species	1570:1603	reduced butyrate-producing species	1570:1603	Additionally, persistent alterations in the gut microbiota composition were observed at P56, including reduced butyrate-producing species.
32726162	7	50	from	expression	1398:1407	arg1	hippocampus					1454:1464	the hippocampus	1450:1464	the hippocampus	1450:1464	Behavioral deficits were found in conjunction with neuroinflammation, decreased neurogenesis, and altered expression of pattern recognition receptor genes in the hippocampus.
32726162	8	51	theme	microbiota	1515:1524	arg1	composition					1526:1536	the gut microbiota composition	1507:1536	the gut microbiota composition	1507:1536	Additionally, persistent alterations in the gut microbiota composition were observed at P56, including reduced butyrate-producing species.
32726162	7	52	theme	decreased	1362:1370	arg1	neurogenesis					1372:1383	decreased neurogenesis	1362:1383	decreased neurogenesis	1362:1383	Behavioral deficits were found in conjunction with neuroinflammation, decreased neurogenesis, and altered expression of pattern recognition receptor genes in the hippocampus.
32726162	10	53	theme	long-lasting	2146:2157	arg1	deficits					2159:2166	long-lasting deficits	2146:2166	long-lasting deficits in the MGB axis in adulthood (P56)	2146:2201	Early-life DSS leads to acute colonic inflammation, similar to adult mice; however, it results in long-lasting deficits in the MGB axis in adulthood (P56), in contrast to the transient deficits seen in adult DSS.
32726162	9	54	theme	long-lasting	1802:1813	arg1	impacts					1815:1821	long-lasting impacts	1802:1821	long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56)	1802:2045	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	11	55	theme	unique	2287:2292	arg1	features					2294:2301	the unique features	2283:2301	the unique features of pediatric inflammatory bowel disease	2283:2341	This model highlights the unique features of pediatric inflammatory bowel disease.
32726162	6	56	theme	novel	1082:1086	arg1	task					1107:1110	novel object recognition task	1082:1110	novel object recognition task	1082:1110	Despite resolution of acute disease, cognitive deficits (novel object recognition task) and anxiety-like behavior (light/dark box) were identified in the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning.
32726162	6	56	theme	novel	1082:1086	arg1	deficits					1072:1079	cognitive deficits	1062:1079	cognitive deficits (novel object recognition task)	1062:1111	Despite resolution of acute disease, cognitive deficits (novel object recognition task) and anxiety-like behavior (light/dark box) were identified in the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning.
32726162	0	57	theme	bowel	41:45	arg1	disease					47:53	pediatric inflammatory bowel disease	18:53	pediatric inflammatory bowel disease	18:53	A murine model of pediatric inflammatory bowel disease causes microbiota-gut-brain axis deficits in adulthood.
32726162	10	58	theme	MGB	2175:2177	arg1	axis					2179:2182	the MGB axis	2171:2182	the MGB axis in adulthood (P56)	2171:2201	Early-life DSS leads to acute colonic inflammation, similar to adult mice; however, it results in long-lasting deficits in the MGB axis in adulthood (P56), in contrast to the transient deficits seen in adult DSS.
32726162	9	59	theme	microbiota-gut-brain	1830:1849	arg1	axis					1857:1860	the microbiota-gut-brain (MGB) axis	1826:1860	the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56)	1826:2045	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	9	59	theme	microbiota-gut-brain	1830:1849	arg1	MGB					1852:1854	MGB	1852:1854	MGB	1852:1854	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	4	60	theme	microbiota-gut-brain	649:668	arg1	deficits					681:688	microbiota-gut-brain (MGB) axis deficits	649:688	microbiota-gut-brain (MGB) axis deficits following induction of colitis in a pediatric setting	649:742	Herein we describe microbiota-gut-brain (MGB) axis deficits following induction of colitis in a pediatric setting.
32726162	9	61	from	inflammation	1984:1995	arg1	P56					2042:2044	P56	2042:2044	P56	2042:2044	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	9	61	from	inflammation	1984:1995	arg1	adulthood					2031:2039	adulthood	2031:2039	adulthood (P56)	2031:2045	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	7	62	theme	Behavioral	1292:1301	arg1	deficits					1303:1310	Behavioral deficits	1292:1310	Behavioral deficits	1292:1310	Behavioral deficits were found in conjunction with neuroinflammation, decreased neurogenesis, and altered expression of pattern recognition receptor genes in the hippocampus.
32726162	6	63	theme	acute	1047:1051	arg1	disease					1053:1059	acute disease	1047:1059	acute disease	1047:1059	Despite resolution of acute disease, cognitive deficits (novel object recognition task) and anxiety-like behavior (light/dark box) were identified in the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning.
32726162	6	64	theme	open	1223:1226	arg1	behavior					1213:1220	exploratory behavior	1201:1220	exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning	1201:1289	Despite resolution of acute disease, cognitive deficits (novel object recognition task) and anxiety-like behavior (light/dark box) were identified in the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning.
32726162	6	64	theme	open	1223:1226	arg1	test					1234:1237	open field test	1223:1237	open field test	1223:1237	Despite resolution of acute disease, cognitive deficits (novel object recognition task) and anxiety-like behavior (light/dark box) were identified in the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning.
32726162	1	65	theme	psychological	215:227	arg1	deficits					243:250	comorbid psychological and cognitive deficits	206:250	comorbid psychological and cognitive deficits	206:250	Inflammatory bowel diseases (IBDs) are chronic intestinal diseases, frequently associated with comorbid psychological and cognitive deficits.
32726162	7	66	from	neurogenesis	1372:1383	arg1	hippocampus					1454:1464	the hippocampus	1450:1464	the hippocampus	1450:1464	Behavioral deficits were found in conjunction with neuroinflammation, decreased neurogenesis, and altered expression of pattern recognition receptor genes in the hippocampus.
32726162	5	67	theme	adult	1007:1011	arg1	mice					1019:1022	adult (P56) mice	1007:1022	adult (P56) mice	1007:1022	Acute colitis was induced by administration of 2% dextran sodium sulfate (DSS) for 5 days starting at weaning [postnatal day (P)21] causing reduced weight gain, colonic shortening, and colonic inflammation by 8 days post-DSS (P29), which were mostly resolved in adult (P56) mice.
32726162	5	67	theme	adult	1007:1011	arg1	P56					1014:1016	P56	1014:1016	P56	1014:1016	Acute colitis was induced by administration of 2% dextran sodium sulfate (DSS) for 5 days starting at weaning [postnatal day (P)21] causing reduced weight gain, colonic shortening, and colonic inflammation by 8 days post-DSS (P29), which were mostly resolved in adult (P56) mice.
32726162	6	68	from	changes	1190:1196	arg1	behavior					1213:1220	exploratory behavior	1201:1220	exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning	1201:1289	Despite resolution of acute disease, cognitive deficits (novel object recognition task) and anxiety-like behavior (light/dark box) were identified in the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning.
32726162	6	68	from	changes	1190:1196	arg1	test					1234:1237	open field test	1223:1237	open field test	1223:1237	Despite resolution of acute disease, cognitive deficits (novel object recognition task) and anxiety-like behavior (light/dark box) were identified in the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning.
32726162	6	69	theme	anxiety-like	1117:1128	arg1	behavior					1130:1137	anxiety-like behavior	1117:1137	anxiety-like behavior (light/dark box)	1117:1154	Despite resolution of acute disease, cognitive deficits (novel object recognition task) and anxiety-like behavior (light/dark box) were identified in the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning.
32726162	6	69	theme	anxiety-like	1117:1128	arg1	box					1151:1153	light/dark box	1140:1153	light/dark box	1140:1153	Despite resolution of acute disease, cognitive deficits (novel object recognition task) and anxiety-like behavior (light/dark box) were identified in the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning.
32726162	9	70	theme	low-dose	1890:1897	arg1	sulfate					1914:1920	low-dose dextran sodium sulfate	1890:1920	low-dose dextran sodium sulfate (DSS)	1890:1926	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	9	70	theme	low-dose	1890:1897	arg1	DSS					1923:1925	DSS	1923:1925	DSS	1923:1925	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	3	71	theme	IBD	618:620	arg1	models					622:627	murine pediatric IBD models	601:627	murine pediatric IBD models	601:627	The mechanism(s) through which these extraintestinal deficits develop remain unknown, and the study of these phenomenon is hampered by a lack of murine pediatric IBD models.
32726162	10	72	theme	Early-life	2048:2057	arg1	DSS					2059:2061	Early-life DSS	2048:2061	Early-life DSS	2048:2061	Early-life DSS leads to acute colonic inflammation, similar to adult mice; however, it results in long-lasting deficits in the MGB axis in adulthood (P56), in contrast to the transient deficits seen in adult DSS.
32726162	9	73	theme	sodium	1907:1912	arg1	sulfate					1914:1920	low-dose dextran sodium sulfate	1890:1920	low-dose dextran sodium sulfate (DSS)	1890:1926	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	9	73	theme	sodium	1907:1912	arg1	DSS					1923:1925	DSS	1923:1925	DSS	1923:1925	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	0	74	theme	microbiota-gut-brain	62:81	arg1	deficits					88:95	microbiota-gut-brain axis deficits	62:95	microbiota-gut-brain axis deficits	62:95	A murine model of pediatric inflammatory bowel disease causes microbiota-gut-brain axis deficits in adulthood.
32726162	3	75	theme	murine	601:606	arg1	models					622:627	murine pediatric IBD models	601:627	murine pediatric IBD models	601:627	The mechanism(s) through which these extraintestinal deficits develop remain unknown, and the study of these phenomenon is hampered by a lack of murine pediatric IBD models.
32726162	5	76	theme	[postnatal	855:864	arg1	P					871:871	P	871:871	P	871:871	Acute colitis was induced by administration of 2% dextran sodium sulfate (DSS) for 5 days starting at weaning [postnatal day (P)21] causing reduced weight gain, colonic shortening, and colonic inflammation by 8 days post-DSS (P29), which were mostly resolved in adult (P56) mice.
32726162	5	76	theme	[postnatal	855:864	arg1	day					866:868	[postnatal day (P)21]	855:875	[postnatal day (P)21] causing reduced weight gain, colonic shortening, and colonic inflammation by 8 days post-DSS (P29), which were mostly resolved in adult (P56) mice	855:1022	Acute colitis was induced by administration of 2% dextran sodium sulfate (DSS) for 5 days starting at weaning [postnatal day (P)21] causing reduced weight gain, colonic shortening, and colonic inflammation by 8 days post-DSS (P29), which were mostly resolved in adult (P56) mice.
32726162	10	77	theme	transient	2223:2231	arg1	deficits					2233:2240	the transient deficits	2219:2240	the transient deficits seen in adult DSS	2219:2258	Early-life DSS leads to acute colonic inflammation, similar to adult mice; however, it results in long-lasting deficits in the MGB axis in adulthood (P56), in contrast to the transient deficits seen in adult DSS.
32726162	11	78	theme	bowel	2329:2333	arg1	disease					2335:2341	pediatric inflammatory bowel disease	2306:2341	pediatric inflammatory bowel disease	2306:2341	This model highlights the unique features of pediatric inflammatory bowel disease.
32726162	6	79	theme	recognition	1095:1105	arg1	task					1107:1110	novel object recognition task	1082:1110	novel object recognition task	1082:1110	Despite resolution of acute disease, cognitive deficits (novel object recognition task) and anxiety-like behavior (light/dark box) were identified in the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning.
32726162	6	79	theme	recognition	1095:1105	arg1	deficits					1072:1079	cognitive deficits	1062:1079	cognitive deficits (novel object recognition task)	1062:1111	Despite resolution of acute disease, cognitive deficits (novel object recognition task) and anxiety-like behavior (light/dark box) were identified in the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning.
32726162	10	80	theme	colonic	2078:2084	arg1	inflammation					2086:2097	acute colonic inflammation	2072:2097	acute colonic inflammation	2072:2097	Early-life DSS leads to acute colonic inflammation, similar to adult mice; however, it results in long-lasting deficits in the MGB axis in adulthood (P56), in contrast to the transient deficits seen in adult DSS.
32726162	4	81	theme	axis	676:679	arg1	deficits					681:688	microbiota-gut-brain (MGB) axis deficits	649:688	microbiota-gut-brain (MGB) axis deficits following induction of colitis in a pediatric setting	649:742	Herein we describe microbiota-gut-brain (MGB) axis deficits following induction of colitis in a pediatric setting.
32726162	10	82	theme	adult	2250:2254	arg1	DSS					2256:2258	adult DSS	2250:2258	adult DSS	2250:2258	Early-life DSS leads to acute colonic inflammation, similar to adult mice; however, it results in long-lasting deficits in the MGB axis in adulthood (P56), in contrast to the transient deficits seen in adult DSS.
32726162	1	83	theme	chronic	150:156	arg1	diseases					169:176	chronic intestinal diseases	150:176	chronic intestinal diseases	150:176	Inflammatory bowel diseases (IBDs) are chronic intestinal diseases, frequently associated with comorbid psychological and cognitive deficits.
32726162	1	83	theme	chronic	150:156	arg1	diseases					130:137	Inflammatory bowel diseases	111:137	Inflammatory bowel diseases (IBDs)	111:144	Inflammatory bowel diseases (IBDs) are chronic intestinal diseases, frequently associated with comorbid psychological and cognitive deficits.
32726162	9	84	theme	MGB	1680:1682	arg1	deficits					1689:1696	MGB axis deficits	1680:1696	MGB axis deficits	1680:1696	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	11	85	theme	pediatric	2306:2314	arg1	disease					2335:2341	pediatric inflammatory bowel disease	2306:2341	pediatric inflammatory bowel disease	2306:2341	This model highlights the unique features of pediatric inflammatory bowel disease.
32726162	9	86	from	dysbiosis	1965:1973	arg1	P56					2042:2044	P56	2042:2044	P56	2042:2044	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	9	86	from	dysbiosis	1965:1973	arg1	adulthood					2031:2039	adulthood	2031:2039	adulthood (P56)	2031:2045	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	5	87	theme	colonic	906:912	arg1	shortening					914:923	colonic shortening	906:923	colonic shortening	906:923	Acute colitis was induced by administration of 2% dextran sodium sulfate (DSS) for 5 days starting at weaning [postnatal day (P)21] causing reduced weight gain, colonic shortening, and colonic inflammation by 8 days post-DSS (P29), which were mostly resolved in adult (P56) mice.
32726162	2	88	theme	memory	317:322	arg1	impairments					324:334	memory impairments	317:334	memory impairments	317:334	These neuropsychiatric effects include anxiety, depression, and memory impairments that can be seen both during active disease and following remission and are more frequently seen in pediatric patients.
32726162	9	89	theme	deficits	1689:1696	arg1	presence					1668:1675	the presence	1664:1675	the presence of MGB axis deficits	1664:1696	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	9	90	theme	weaning	1931:1937	arg1	dysbiosis					1965:1973	gut dysbiosis	1961:1973	gut dysbiosis	1961:1973	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	9	90	theme	weaning	1931:1937	arg1	P21					1945:1947	P21	1945:1947	P21	1945:1947	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	9	90	theme	weaning	1931:1937	arg1	mice					1939:1942	weaning mice	1931:1942	weaning mice (P21)	1931:1948	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	9	90	theme	weaning	1931:1937	arg1	deficits					2019:2026	brain/behavioral deficits	2002:2026	brain/behavioral deficits in adulthood (P56)	2002:2045	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	9	90	theme	weaning	1931:1937	arg1	inflammation					1984:1995	colonic inflammation	1976:1995	colonic inflammation	1976:1995	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	3	91	theme	mechanism	460:468	arg1	s					470:470	The mechanism(s)	456:471	The mechanism(s) through which these extraintestinal deficits develop	456:524	The mechanism(s) through which these extraintestinal deficits develop remain unknown, and the study of these phenomenon is hampered by a lack of murine pediatric IBD models.
32726162	5	92	theme	dextran	795:801	arg1	DSS					819:821	DSS	819:821	DSS	819:821	Acute colitis was induced by administration of 2% dextran sodium sulfate (DSS) for 5 days starting at weaning [postnatal day (P)21] causing reduced weight gain, colonic shortening, and colonic inflammation by 8 days post-DSS (P29), which were mostly resolved in adult (P56) mice.
32726162	5	92	theme	dextran	795:801	arg1	sulfate					810:816	2% dextran sodium sulfate	792:816	2% dextran sodium sulfate (DSS)	792:822	Acute colitis was induced by administration of 2% dextran sodium sulfate (DSS) for 5 days starting at weaning [postnatal day (P)21] causing reduced weight gain, colonic shortening, and colonic inflammation by 8 days post-DSS (P29), which were mostly resolved in adult (P56) mice.
32726162	5	93	theme	reduced	885:891	arg1	gain					900:903	reduced weight gain	885:903	reduced weight gain	885:903	Acute colitis was induced by administration of 2% dextran sodium sulfate (DSS) for 5 days starting at weaning [postnatal day (P)21] causing reduced weight gain, colonic shortening, and colonic inflammation by 8 days post-DSS (P29), which were mostly resolved in adult (P56) mice.
32726162	9	94	theme	colitis	1721:1727	arg1	induction					1708:1716	induction	1708:1716	induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56)	1708:2045	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	4	95	theme	pediatric	726:734	arg1	setting					736:742	a pediatric setting	724:742	a pediatric setting	724:742	Herein we describe microbiota-gut-brain (MGB) axis deficits following induction of colitis in a pediatric setting.
32726162	5	96	theme	sulfate	810:816	arg1	administration					774:787	administration	774:787	administration of 2% dextran sodium sulfate (DSS)	774:822	Acute colitis was induced by administration of 2% dextran sodium sulfate (DSS) for 5 days starting at weaning [postnatal day (P)21] causing reduced weight gain, colonic shortening, and colonic inflammation by 8 days post-DSS (P29), which were mostly resolved in adult (P56) mice.
32726162	10	97	from	deficits	2159:2166	arg1	axis					2179:2182	the MGB axis	2171:2182	the MGB axis in adulthood (P56)	2171:2201	Early-life DSS leads to acute colonic inflammation, similar to adult mice; however, it results in long-lasting deficits in the MGB axis in adulthood (P56), in contrast to the transient deficits seen in adult DSS.
32726162	0	98	theme	inflammatory	28:39	arg1	disease					47:53	pediatric inflammatory bowel disease	18:53	pediatric inflammatory bowel disease	18:53	A murine model of pediatric inflammatory bowel disease causes microbiota-gut-brain axis deficits in adulthood.
32726162	5	99	theme	days	956:959	arg1	post-DSS					961:968	8 days post-DSS (P29)	954:974	8 days post-DSS (P29)	954:974	Acute colitis was induced by administration of 2% dextran sodium sulfate (DSS) for 5 days starting at weaning [postnatal day (P)21] causing reduced weight gain, colonic shortening, and colonic inflammation by 8 days post-DSS (P29), which were mostly resolved in adult (P56) mice.
32726162	8	100	theme	persistent	1481:1490	arg1	alterations					1492:1502	persistent alterations	1481:1502	persistent alterations in the gut microbiota composition	1481:1536	Additionally, persistent alterations in the gut microbiota composition were observed at P56, including reduced butyrate-producing species.
32726162	0	101	theme	disease	47:53	arg1	model					9:13	A murine model	0:13	A murine model of pediatric inflammatory bowel disease	0:53	A murine model of pediatric inflammatory bowel disease causes microbiota-gut-brain axis deficits in adulthood.
32726162	7	102	located	found	1317:1321	arg1	conjunction					1326:1336	conjunction	1326:1336	conjunction with neuroinflammation, decreased neurogenesis, and altered expression of pattern recognition receptor genes in the hippocampus	1326:1464	Behavioral deficits were found in conjunction with neuroinflammation, decreased neurogenesis, and altered expression of pattern recognition receptor genes in the hippocampus.
32726162	7	102	located	found	1317:1321	arg2	deficits					1303:1310	Behavioral deficits	1292:1310	Behavioral deficits	1292:1310	Behavioral deficits were found in conjunction with neuroinflammation, decreased neurogenesis, and altered expression of pattern recognition receptor genes in the hippocampus.
32726162	7	103	theme	receptor	1432:1439	arg1	genes					1441:1445	pattern recognition receptor genes	1412:1445	pattern recognition receptor genes	1412:1445	Behavioral deficits were found in conjunction with neuroinflammation, decreased neurogenesis, and altered expression of pattern recognition receptor genes in the hippocampus.
32726162	10	104	from	axis	2179:2182	arg1	P56					2198:2200	P56	2198:2200	P56	2198:2200	Early-life DSS leads to acute colonic inflammation, similar to adult mice; however, it results in long-lasting deficits in the MGB axis in adulthood (P56), in contrast to the transient deficits seen in adult DSS.
32726162	10	104	from	axis	2179:2182	arg1	adulthood					2187:2195	adulthood	2187:2195	adulthood (P56)	2187:2201	Early-life DSS leads to acute colonic inflammation, similar to adult mice; however, it results in long-lasting deficits in the MGB axis in adulthood (P56), in contrast to the transient deficits seen in adult DSS.
32726162	9	105	theme	colonic	1976:1982	arg1	inflammation					1984:1995	colonic inflammation	1976:1995	colonic inflammation	1976:1995	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	7	106	with	conjunction	1326:1336	arg1	neuroinflammation					1343:1359	neuroinflammation	1343:1359	neuroinflammation	1343:1359	Behavioral deficits were found in conjunction with neuroinflammation, decreased neurogenesis, and altered expression of pattern recognition receptor genes in the hippocampus.
32726162	7	106	with	conjunction	1326:1336	arg1	neurogenesis					1372:1383	decreased neurogenesis	1362:1383	decreased neurogenesis	1362:1383	Behavioral deficits were found in conjunction with neuroinflammation, decreased neurogenesis, and altered expression of pattern recognition receptor genes in the hippocampus.
32726162	7	106	with	conjunction	1326:1336	arg1	expression					1398:1407	altered expression	1390:1407	altered expression of pattern recognition receptor genes in the hippocampus	1390:1464	Behavioral deficits were found in conjunction with neuroinflammation, decreased neurogenesis, and altered expression of pattern recognition receptor genes in the hippocampus.
32726162	10	107	dep	deficits	2233:2240	arg1	contrast					2207:2214	contrast	2207:2214	contrast	2207:2214	Early-life DSS leads to acute colonic inflammation, similar to adult mice; however, it results in long-lasting deficits in the MGB axis in adulthood (P56), in contrast to the transient deficits seen in adult DSS.
32726162	7	108	theme	pattern	1412:1418	arg1	receptor					1432:1439	pattern recognition receptor	1412:1439	pattern recognition receptor genes	1412:1445	Behavioral deficits were found in conjunction with neuroinflammation, decreased neurogenesis, and altered expression of pattern recognition receptor genes in the hippocampus.
32726162	5	109	theme	colonic	930:936	arg1	inflammation					938:949	colonic inflammation	930:949	colonic inflammation by 8 days post-DSS (P29), which were mostly resolved in adult (P56) mice	930:1022	Acute colitis was induced by administration of 2% dextran sodium sulfate (DSS) for 5 days starting at weaning [postnatal day (P)21] causing reduced weight gain, colonic shortening, and colonic inflammation by 8 days post-DSS (P29), which were mostly resolved in adult (P56) mice.
32726162	8	110	theme	gut	1511:1513	arg1	composition					1526:1536	the gut microbiota composition	1507:1536	the gut microbiota composition	1507:1536	Additionally, persistent alterations in the gut microbiota composition were observed at P56, including reduced butyrate-producing species.
32726162	8	111	theme	butyrate-producing	1578:1595	arg1	species					1597:1603	reduced butyrate-producing species	1570:1603	reduced butyrate-producing species	1570:1603	Additionally, persistent alterations in the gut microbiota composition were observed at P56, including reduced butyrate-producing species.
32726162	9	112	theme	brain/behavioral	2002:2017	arg1	deficits					2019:2026	brain/behavioral deficits	2002:2026	brain/behavioral deficits in adulthood (P56)	2002:2045	Taken together, these results describe for the first time the presence of MGB axis deficits following induction of colitis at weaning, which persist in adulthood.NEW & NOTEWORTHY Here we describe long-lasting impacts on the microbiota-gut-brain (MGB) axis following administration of low-dose dextran sodium sulfate (DSS) to weaning mice (P21), including gut dysbiosis, colonic inflammation, and brain/behavioral deficits in adulthood (P56).
32726162	0	113	theme	murine	2:7	arg1	model					9:13	A murine model	0:13	A murine model of pediatric inflammatory bowel disease	0:53	A murine model of pediatric inflammatory bowel disease causes microbiota-gut-brain axis deficits in adulthood.
32726162	4	114	from	induction	700:708	arg1	setting					736:742	a pediatric setting	724:742	a pediatric setting	724:742	Herein we describe microbiota-gut-brain (MGB) axis deficits following induction of colitis in a pediatric setting.
32726162	6	115	from	absence	1179:1185	arg1	behavior					1213:1220	exploratory behavior	1201:1220	exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning	1201:1289	Despite resolution of acute disease, cognitive deficits (novel object recognition task) and anxiety-like behavior (light/dark box) were identified in the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning.
32726162	6	115	from	absence	1179:1185	arg1	test					1234:1237	open field test	1223:1237	open field test	1223:1237	Despite resolution of acute disease, cognitive deficits (novel object recognition task) and anxiety-like behavior (light/dark box) were identified in the absence of changes in exploratory behavior (open field test) in P56 mice previously treated with DSS at weaning.
31985395	8	0	theme	meso-2,6-diaminopimelic	921:943	arg1	acid					945:948	meso-2,6-diaminopimelic acid	921:948	meso-2,6-diaminopimelic acid	921:948	The cell wall peptidoglycan of SYSU G01001T was meso-2,6-diaminopimelic acid and MK-7 was the only respiratory quinone.
31985395	8	0	theme	meso-2,6-diaminopimelic	921:943	arg1	peptidoglycan					887:899	The cell wall peptidoglycan	873:899	The cell wall peptidoglycan of SYSU G01001T	873:915	The cell wall peptidoglycan of SYSU G01001T was meso-2,6-diaminopimelic acid and MK-7 was the only respiratory quinone.
31985395	6	1	theme	Paenibacillus	636:648	arg1	azotifigens					650:660	Paenibacillus azotifigens	636:660	Paenibacillus azotifigens (97.5 %)	636:669	The 16S rRNA gene sequence similarity between SYSU G01001T and SY-13 was 99.6 % but these strains shared low sequence similarity with Paenibacillus azotifigens (97.5 %) indicating that they represented a novel species.
31985395	6	1	theme	Paenibacillus	636:648	arg1	%					668:668	97.5 %	663:668	97.5 %	663:668	The 16S rRNA gene sequence similarity between SYSU G01001T and SY-13 was 99.6 % but these strains shared low sequence similarity with Paenibacillus azotifigens (97.5 %) indicating that they represented a novel species.
31985395	11	2	theme	nucleotide	1486:1495	arg1	values					1351:1356	The average nucleotide identity (ANIb and ANIm) values	1303:1356	The average nucleotide identity (ANIb and ANIm) values between SYSU G01001T and Paenibacillus azotifigens LMG 29963T	1303:1418	The average nucleotide identity (ANIb and ANIm) values between SYSU G01001T and Paenibacillus azotifigens LMG 29963T were below the cut-off level (95-96 %) recommended as the average nucleotide identity (ANI) criterion for interspecies identity.
31985395	11	2	theme	nucleotide	1486:1495	arg1	criterion					1512:1520	the average nucleotide identity (ANI) criterion	1474:1520	the average nucleotide identity (ANI) criterion for interspecies identity	1474:1546	The average nucleotide identity (ANIb and ANIm) values between SYSU G01001T and Paenibacillus azotifigens LMG 29963T were below the cut-off level (95-96 %) recommended as the average nucleotide identity (ANI) criterion for interspecies identity.
31985395	9	3	theme	unidentified	1142:1153	arg1	APL					1176:1178	APL	1176:1178	APL	1176:1178	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two unidentified aminolipids (AL), two unidentified amino phospholipids (APL), an unidentified phospholipid (PL) and an unidentified polar lipid (L).
31985395	9	3	theme	unidentified	1142:1153	arg1	phospholipids					1161:1173	two unidentified amino phospholipids	1138:1173	two unidentified amino phospholipids (APL)	1138:1179	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two unidentified aminolipids (AL), two unidentified amino phospholipids (APL), an unidentified phospholipid (PL) and an unidentified polar lipid (L).
31985395	12	4	theme	above	1569:1573	arg1	results					1575:1581	the above results	1565:1581	the above results	1565:1581	On the basis of the above results strain SYSU G01001T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus tepidiphilus sp.
31985395	11	5	theme	ANI	1507:1509	arg1	values					1351:1356	The average nucleotide identity (ANIb and ANIm) values	1303:1356	The average nucleotide identity (ANIb and ANIm) values between SYSU G01001T and Paenibacillus azotifigens LMG 29963T	1303:1418	The average nucleotide identity (ANIb and ANIm) values between SYSU G01001T and Paenibacillus azotifigens LMG 29963T were below the cut-off level (95-96 %) recommended as the average nucleotide identity (ANI) criterion for interspecies identity.
31985395	11	5	theme	ANI	1507:1509	arg1	criterion					1512:1520	the average nucleotide identity (ANI) criterion	1474:1520	the average nucleotide identity (ANI) criterion for interspecies identity	1474:1546	The average nucleotide identity (ANIb and ANIm) values between SYSU G01001T and Paenibacillus azotifigens LMG 29963T were below the cut-off level (95-96 %) recommended as the average nucleotide identity (ANI) criterion for interspecies identity.
31985395	11	6	theme	LMG	1409:1411	arg1	29963T					1413:1418	LMG 29963T	1409:1418	LMG 29963T	1409:1418	The average nucleotide identity (ANIb and ANIm) values between SYSU G01001T and Paenibacillus azotifigens LMG 29963T were below the cut-off level (95-96 %) recommended as the average nucleotide identity (ANI) criterion for interspecies identity.
31985395	10	7	theme	genomic	1276:1282	arg1	DNA					1284:1286	the genomic DNA	1272:1286	the genomic DNA	1272:1286	The G+C content of the genomic DNA was 53.9 mol%.
31985395	7	8	theme	species	864:870	arg1	strain					850:855	a second strain	841:855	a second strain of the species	841:870	On the basis of the results, SYSU G01001T was selected for further investigations and SY-13 was considered to represent a second strain of the species.
31985395	7	9	dep	results	741:747	arg1	basis					728:732	basis	728:732	basis	728:732	On the basis of the results, SYSU G01001T was selected for further investigations and SY-13 was considered to represent a second strain of the species.
31985395	7	9	dep	results	741:747	arg1	the					724:726	the	724:726	the	724:726	On the basis of the results, SYSU G01001T was selected for further investigations and SY-13 was considered to represent a second strain of the species.
31985395	11	10	theme	average	1478:1484	arg1	values					1351:1356	The average nucleotide identity (ANIb and ANIm) values	1303:1356	The average nucleotide identity (ANIb and ANIm) values between SYSU G01001T and Paenibacillus azotifigens LMG 29963T	1303:1418	The average nucleotide identity (ANIb and ANIm) values between SYSU G01001T and Paenibacillus azotifigens LMG 29963T were below the cut-off level (95-96 %) recommended as the average nucleotide identity (ANI) criterion for interspecies identity.
31985395	11	10	theme	average	1478:1484	arg1	criterion					1512:1520	the average nucleotide identity (ANI) criterion	1474:1520	the average nucleotide identity (ANI) criterion for interspecies identity	1474:1546	The average nucleotide identity (ANIb and ANIm) values between SYSU G01001T and Paenibacillus azotifigens LMG 29963T were below the cut-off level (95-96 %) recommended as the average nucleotide identity (ANI) criterion for interspecies identity.
31985395	2	11	theme	microbial	128:136	arg1	analysis					148:155	microbial diversity analysis	128:155	microbial diversity analysis of Tengchong hot spring, Yunnan province, south-west PR China	128:217	China is a hotspot for hot springs and during microbial diversity analysis of Tengchong hot spring, Yunnan province, south-west PR China, two strains designated SYSU G01001T and SY-13 were isolated.
31985395	14	12	theme	type	1730:1733	arg1	strain					1735:1740	The type strain	1726:1740	The type strain	1726:1740	The type strain is SYSU G01001T (=KCTC 33952T=CGMCC 1.13870T).
31985395	14	12	theme	type	1730:1733	arg1	G01001T					1750:1756	SYSU G01001T	1745:1756	SYSU G01001T (=KCTC 33952T=CGMCC 1.13870T)	1745:1786	The type strain is SYSU G01001T (=KCTC 33952T=CGMCC 1.13870T).
31985395	8	13	theme	wall	882:885	arg1	acid					945:948	meso-2,6-diaminopimelic acid	921:948	meso-2,6-diaminopimelic acid	921:948	The cell wall peptidoglycan of SYSU G01001T was meso-2,6-diaminopimelic acid and MK-7 was the only respiratory quinone.
31985395	8	13	theme	wall	882:885	arg1	peptidoglycan					887:899	The cell wall peptidoglycan	873:899	The cell wall peptidoglycan of SYSU G01001T	873:915	The cell wall peptidoglycan of SYSU G01001T was meso-2,6-diaminopimelic acid and MK-7 was the only respiratory quinone.
31985395	11	14	theme	identity	1497:1504	arg1	values					1351:1356	The average nucleotide identity (ANIb and ANIm) values	1303:1356	The average nucleotide identity (ANIb and ANIm) values between SYSU G01001T and Paenibacillus azotifigens LMG 29963T	1303:1418	The average nucleotide identity (ANIb and ANIm) values between SYSU G01001T and Paenibacillus azotifigens LMG 29963T were below the cut-off level (95-96 %) recommended as the average nucleotide identity (ANI) criterion for interspecies identity.
31985395	11	14	theme	identity	1497:1504	arg1	criterion					1512:1520	the average nucleotide identity (ANI) criterion	1474:1520	the average nucleotide identity (ANI) criterion for interspecies identity	1474:1546	The average nucleotide identity (ANIb and ANIm) values between SYSU G01001T and Paenibacillus azotifigens LMG 29963T were below the cut-off level (95-96 %) recommended as the average nucleotide identity (ANI) criterion for interspecies identity.
31985395	8	15	theme	cell	877:880	arg1	acid					945:948	meso-2,6-diaminopimelic acid	921:948	meso-2,6-diaminopimelic acid	921:948	The cell wall peptidoglycan of SYSU G01001T was meso-2,6-diaminopimelic acid and MK-7 was the only respiratory quinone.
31985395	8	15	theme	cell	877:880	arg1	peptidoglycan					887:899	The cell wall peptidoglycan	873:899	The cell wall peptidoglycan of SYSU G01001T	873:915	The cell wall peptidoglycan of SYSU G01001T was meso-2,6-diaminopimelic acid and MK-7 was the only respiratory quinone.
31985395	2	16	theme	SYSU	243:246	arg1	G01001T					248:254	SYSU G01001T	243:254	SYSU G01001T	243:254	China is a hotspot for hot springs and during microbial diversity analysis of Tengchong hot spring, Yunnan province, south-west PR China, two strains designated SYSU G01001T and SY-13 were isolated.
31985395	9	17	theme	unidentified	1185:1196	arg1	PL					1212:1213	PL	1212:1213	PL	1212:1213	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two unidentified aminolipids (AL), two unidentified amino phospholipids (APL), an unidentified phospholipid (PL) and an unidentified polar lipid (L).
31985395	9	17	theme	unidentified	1185:1196	arg1	phospholipid					1198:1209	an unidentified phospholipid	1182:1209	an unidentified phospholipid (PL)	1182:1214	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two unidentified aminolipids (AL), two unidentified amino phospholipids (APL), an unidentified phospholipid (PL) and an unidentified polar lipid (L).
31985395	12	18	theme	tepidiphilus	1691:1702	arg1	sp					1704:1705	the name Paenibacillus tepidiphilus sp	1668:1705	the name Paenibacillus tepidiphilus sp	1668:1705	On the basis of the above results strain SYSU G01001T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus tepidiphilus sp.
31985395	6	19	theme	novel	706:710	arg1	species					712:718	a novel species	704:718	a novel species	704:718	The 16S rRNA gene sequence similarity between SYSU G01001T and SY-13 was 99.6 % but these strains shared low sequence similarity with Paenibacillus azotifigens (97.5 %) indicating that they represented a novel species.
31985395	1	20	theme	tepid	68:72	arg1	spring					74:79	a tepid spring	66:79	a tepid spring	66:79	nov., isolated from a tepid spring.
31985395	5	21	theme	optimum	453:459	arg1	pH					441:442	pH 6.0-9.0	441:450	pH 6.0-9.0 (optimum pH 8.0)	441:467	SYSU G01001T and SY-13 grew at pH 6.0-9.0 (optimum pH 8.0) and at 23-37 °C (optimum 28 °C).
31985395	5	21	theme	optimum	453:459	arg1	pH					461:462	optimum pH 8.0	453:466	optimum pH 8.0	453:466	SYSU G01001T and SY-13 grew at pH 6.0-9.0 (optimum pH 8.0) and at 23-37 °C (optimum 28 °C).
31985395	9	22	theme	amino	1155:1159	arg1	APL					1176:1178	APL	1176:1178	APL	1176:1178	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two unidentified aminolipids (AL), two unidentified amino phospholipids (APL), an unidentified phospholipid (PL) and an unidentified polar lipid (L).
31985395	9	22	theme	amino	1155:1159	arg1	phospholipids					1161:1173	two unidentified amino phospholipids	1138:1173	two unidentified amino phospholipids (APL)	1138:1179	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two unidentified aminolipids (AL), two unidentified amino phospholipids (APL), an unidentified phospholipid (PL) and an unidentified polar lipid (L).
31985395	8	23	theme	only	967:970	arg1	quinone					984:990	the only respiratory quinone	963:990	the only respiratory quinone	963:990	The cell wall peptidoglycan of SYSU G01001T was meso-2,6-diaminopimelic acid and MK-7 was the only respiratory quinone.
31985395	8	23	theme	only	967:970	arg1	MK-7					954:957	MK-7	954:957	MK-7	954:957	The cell wall peptidoglycan of SYSU G01001T was meso-2,6-diaminopimelic acid and MK-7 was the only respiratory quinone.
31985395	7	24	theme	second	843:848	arg1	strain					850:855	a second strain	841:855	a second strain of the species	841:870	On the basis of the results, SYSU G01001T was selected for further investigations and SY-13 was considered to represent a second strain of the species.
31985395	6	25	theme	low	607:609	arg1	similarity					620:629	low sequence similarity	607:629	low sequence similarity with Paenibacillus azotifigens (97.5 %)	607:669	The 16S rRNA gene sequence similarity between SYSU G01001T and SY-13 was 99.6 % but these strains shared low sequence similarity with Paenibacillus azotifigens (97.5 %) indicating that they represented a novel species.
31985395	6	26	theme	sequence	520:527	arg1	%					580:580	99.6 %	575:580	99.6 %	575:580	The 16S rRNA gene sequence similarity between SYSU G01001T and SY-13 was 99.6 % but these strains shared low sequence similarity with Paenibacillus azotifigens (97.5 %) indicating that they represented a novel species.
31985395	6	26	theme	sequence	520:527	arg1	similarity					529:538	The 16S rRNA gene sequence similarity	502:538	The 16S rRNA gene sequence similarity between SYSU G01001T and SY-13	502:569	The 16S rRNA gene sequence similarity between SYSU G01001T and SY-13 was 99.6 % but these strains shared low sequence similarity with Paenibacillus azotifigens (97.5 %) indicating that they represented a novel species.
31985395	0	27	theme	tepidiphilus	29:40	arg1	sp					42:43	Paenibacillus tepidiphilus sp	15:43	Paenibacillus tepidiphilus sp	15:43	Description of Paenibacillus tepidiphilus sp.
31985395	8	28	theme	G01001T	909:915	arg1	acid					945:948	meso-2,6-diaminopimelic acid	921:948	meso-2,6-diaminopimelic acid	921:948	The cell wall peptidoglycan of SYSU G01001T was meso-2,6-diaminopimelic acid and MK-7 was the only respiratory quinone.
31985395	8	28	theme	G01001T	909:915	arg1	peptidoglycan					887:899	The cell wall peptidoglycan	873:899	The cell wall peptidoglycan of SYSU G01001T	873:915	The cell wall peptidoglycan of SYSU G01001T was meso-2,6-diaminopimelic acid and MK-7 was the only respiratory quinone.
31985395	9	29	theme	polar	997:1001	arg1	lipids					1003:1008	The polar lipids	993:1008	The polar lipids	993:1008	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two unidentified aminolipids (AL), two unidentified amino phospholipids (APL), an unidentified phospholipid (PL) and an unidentified polar lipid (L).
31985395	9	29	theme	polar	997:1001	arg1	diphosphatidylglycerol					1015:1036	diphosphatidylglycerol	1015:1036	diphosphatidylglycerol (DPG)	1015:1042	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two unidentified aminolipids (AL), two unidentified amino phospholipids (APL), an unidentified phospholipid (PL) and an unidentified polar lipid (L).
31985395	10	30	theme	DNA	1284:1286	arg1	%					1300:1300	53.9 mol%	1292:1300	53.9 mol%	1292:1300	The G+C content of the genomic DNA was 53.9 mol%.
31985395	10	30	theme	DNA	1284:1286	arg1	content					1261:1267	The G+C content	1253:1267	The G+C content of the genomic DNA	1253:1286	The G+C content of the genomic DNA was 53.9 mol%.
31985395	6	31	theme	gene	515:518	arg1	%					580:580	99.6 %	575:580	99.6 %	575:580	The 16S rRNA gene sequence similarity between SYSU G01001T and SY-13 was 99.6 % but these strains shared low sequence similarity with Paenibacillus azotifigens (97.5 %) indicating that they represented a novel species.
31985395	6	31	theme	gene	515:518	arg1	similarity					529:538	The 16S rRNA gene sequence similarity	502:538	The 16S rRNA gene sequence similarity between SYSU G01001T and SY-13	502:569	The 16S rRNA gene sequence similarity between SYSU G01001T and SY-13 was 99.6 % but these strains shared low sequence similarity with Paenibacillus azotifigens (97.5 %) indicating that they represented a novel species.
31985395	10	32	theme	G+C	1257:1259	arg1	%					1300:1300	53.9 mol%	1292:1300	53.9 mol%	1292:1300	The G+C content of the genomic DNA was 53.9 mol%.
31985395	10	32	theme	G+C	1257:1259	arg1	content					1261:1267	The G+C content	1253:1267	The G+C content of the genomic DNA	1253:1286	The G+C content of the genomic DNA was 53.9 mol%.
31985395	14	33	theme	33952T=CGMCC	1765:1776	arg1	1.13870T					1778:1785	=KCTC 33952T=CGMCC 1.13870T	1759:1785	=KCTC 33952T=CGMCC 1.13870T	1759:1785	The type strain is SYSU G01001T (=KCTC 33952T=CGMCC 1.13870T).
31985395	14	33	theme	33952T=CGMCC	1765:1776	arg1	G01001T					1750:1756	SYSU G01001T	1745:1756	SYSU G01001T (=KCTC 33952T=CGMCC 1.13870T)	1745:1786	The type strain is SYSU G01001T (=KCTC 33952T=CGMCC 1.13870T).
31985395	9	34	theme	unidentified	1107:1118	arg1	aminolipids					1120:1130	two unidentified aminolipids	1103:1130	two unidentified aminolipids (AL)	1103:1135	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two unidentified aminolipids (AL), two unidentified amino phospholipids (APL), an unidentified phospholipid (PL) and an unidentified polar lipid (L).
31985395	10	35	theme	53.9 mol	1292:1299	arg1	%					1300:1300	53.9 mol%	1292:1300	53.9 mol%	1292:1300	The G+C content of the genomic DNA was 53.9 mol%.
31985395	10	35	theme	53.9 mol	1292:1299	arg1	content					1261:1267	The G+C content	1253:1267	The G+C content of the genomic DNA	1253:1286	The G+C content of the genomic DNA was 53.9 mol%.
31985395	6	36	theme	rRNA	510:513	arg1	%					580:580	99.6 %	575:580	99.6 %	575:580	The 16S rRNA gene sequence similarity between SYSU G01001T and SY-13 was 99.6 % but these strains shared low sequence similarity with Paenibacillus azotifigens (97.5 %) indicating that they represented a novel species.
31985395	6	36	theme	rRNA	510:513	arg1	similarity					529:538	The 16S rRNA gene sequence similarity	502:538	The 16S rRNA gene sequence similarity between SYSU G01001T and SY-13	502:569	The 16S rRNA gene sequence similarity between SYSU G01001T and SY-13 was 99.6 % but these strains shared low sequence similarity with Paenibacillus azotifigens (97.5 %) indicating that they represented a novel species.
31985395	11	37	dep	identity	1326:1333	arg1	ANIm					1345:1348	ANIm	1345:1348	ANIm	1345:1348	The average nucleotide identity (ANIb and ANIm) values between SYSU G01001T and Paenibacillus azotifigens LMG 29963T were below the cut-off level (95-96 %) recommended as the average nucleotide identity (ANI) criterion for interspecies identity.
31985395	11	37	dep	identity	1326:1333	arg1	ANIb					1336:1339	ANIb	1336:1339	ANIb	1336:1339	The average nucleotide identity (ANIb and ANIm) values between SYSU G01001T and Paenibacillus azotifigens LMG 29963T were below the cut-off level (95-96 %) recommended as the average nucleotide identity (ANI) criterion for interspecies identity.
31985395	8	38	theme	respiratory	972:982	arg1	quinone					984:990	the only respiratory quinone	963:990	the only respiratory quinone	963:990	The cell wall peptidoglycan of SYSU G01001T was meso-2,6-diaminopimelic acid and MK-7 was the only respiratory quinone.
31985395	8	38	theme	respiratory	972:982	arg1	MK-7					954:957	MK-7	954:957	MK-7	954:957	The cell wall peptidoglycan of SYSU G01001T was meso-2,6-diaminopimelic acid and MK-7 was the only respiratory quinone.
31985395	2	39	theme	Tengchong	160:168	arg1	spring					174:179	Tengchong hot spring	160:179	Tengchong hot spring	160:179	China is a hotspot for hot springs and during microbial diversity analysis of Tengchong hot spring, Yunnan province, south-west PR China, two strains designated SYSU G01001T and SY-13 were isolated.
31985395	2	39	theme	Tengchong	160:168	arg1	province					189:196	Yunnan province	182:196	Yunnan province	182:196	China is a hotspot for hot springs and during microbial diversity analysis of Tengchong hot spring, Yunnan province, south-west PR China, two strains designated SYSU G01001T and SY-13 were isolated.
31985395	6	40	theme	16S	506:508	arg1	%					580:580	99.6 %	575:580	99.6 %	575:580	The 16S rRNA gene sequence similarity between SYSU G01001T and SY-13 was 99.6 % but these strains shared low sequence similarity with Paenibacillus azotifigens (97.5 %) indicating that they represented a novel species.
31985395	6	40	theme	16S	506:508	arg1	similarity					529:538	The 16S rRNA gene sequence similarity	502:538	The 16S rRNA gene sequence similarity between SYSU G01001T and SY-13	502:569	The 16S rRNA gene sequence similarity between SYSU G01001T and SY-13 was 99.6 % but these strains shared low sequence similarity with Paenibacillus azotifigens (97.5 %) indicating that they represented a novel species.
31985395	12	41	theme	Paenibacillus	1677:1689	arg1	sp					1704:1705	the name Paenibacillus tepidiphilus sp	1668:1705	the name Paenibacillus tepidiphilus sp	1668:1705	On the basis of the above results strain SYSU G01001T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus tepidiphilus sp.
31985395	2	42	theme	PR	210:211	arg1	province					189:196	Yunnan province	182:196	Yunnan province	182:196	China is a hotspot for hot springs and during microbial diversity analysis of Tengchong hot spring, Yunnan province, south-west PR China, two strains designated SYSU G01001T and SY-13 were isolated.
31985395	2	42	theme	PR	210:211	arg1	China					213:217	south-west PR China	199:217	south-west PR China	199:217	China is a hotspot for hot springs and during microbial diversity analysis of Tengchong hot spring, Yunnan province, south-west PR China, two strains designated SYSU G01001T and SY-13 were isolated.
31985395	11	43	dep	G01001T	1371:1377	arg1	29963T					1413:1418	LMG 29963T	1409:1418	LMG 29963T	1409:1418	The average nucleotide identity (ANIb and ANIm) values between SYSU G01001T and Paenibacillus azotifigens LMG 29963T were below the cut-off level (95-96 %) recommended as the average nucleotide identity (ANI) criterion for interspecies identity.
31985395	0	44	theme	sp	42:43	arg1	Description					0:10	Description	0:10	Description of Paenibacillus tepidiphilus sp.	0:44	Description of Paenibacillus tepidiphilus sp.
31985395	6	45	with	similarity	620:629	arg1	azotifigens					650:660	Paenibacillus azotifigens	636:660	Paenibacillus azotifigens (97.5 %)	636:669	The 16S rRNA gene sequence similarity between SYSU G01001T and SY-13 was 99.6 % but these strains shared low sequence similarity with Paenibacillus azotifigens (97.5 %) indicating that they represented a novel species.
31985395	6	45	with	similarity	620:629	arg1	%					668:668	97.5 %	663:668	97.5 %	663:668	The 16S rRNA gene sequence similarity between SYSU G01001T and SY-13 was 99.6 % but these strains shared low sequence similarity with Paenibacillus azotifigens (97.5 %) indicating that they represented a novel species.
31985395	12	46	dep	results	1575:1581	arg1	basis					1556:1560	basis	1556:1560	basis	1556:1560	On the basis of the above results strain SYSU G01001T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus tepidiphilus sp.
31985395	12	46	dep	results	1575:1581	arg1	the					1552:1554	the	1552:1554	the	1552:1554	On the basis of the above results strain SYSU G01001T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus tepidiphilus sp.
31985395	2	47	theme	south-west	199:208	arg1	province					189:196	Yunnan province	182:196	Yunnan province	182:196	China is a hotspot for hot springs and during microbial diversity analysis of Tengchong hot spring, Yunnan province, south-west PR China, two strains designated SYSU G01001T and SY-13 were isolated.
31985395	2	47	theme	south-west	199:208	arg1	China					213:217	south-west PR China	199:217	south-west PR China	199:217	China is a hotspot for hot springs and during microbial diversity analysis of Tengchong hot spring, Yunnan province, south-west PR China, two strains designated SYSU G01001T and SY-13 were isolated.
31985395	14	48	theme	=KCTC	1759:1763	arg1	1.13870T					1778:1785	=KCTC 33952T=CGMCC 1.13870T	1759:1785	=KCTC 33952T=CGMCC 1.13870T	1759:1785	The type strain is SYSU G01001T (=KCTC 33952T=CGMCC 1.13870T).
31985395	14	48	theme	=KCTC	1759:1763	arg1	G01001T					1750:1756	SYSU G01001T	1745:1756	SYSU G01001T (=KCTC 33952T=CGMCC 1.13870T)	1745:1786	The type strain is SYSU G01001T (=KCTC 33952T=CGMCC 1.13870T).
31985395	9	49	theme	unidentified	1223:1234	arg1	L					1249:1249	L	1249:1249	L	1249:1249	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two unidentified aminolipids (AL), two unidentified amino phospholipids (APL), an unidentified phospholipid (PL) and an unidentified polar lipid (L).
31985395	9	49	theme	unidentified	1223:1234	arg1	lipid					1242:1246	an unidentified polar lipid	1220:1246	an unidentified polar lipid (L)	1220:1250	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two unidentified aminolipids (AL), two unidentified amino phospholipids (APL), an unidentified phospholipid (PL) and an unidentified polar lipid (L).
31985395	12	50	theme	Paenibacillus	1643:1655	arg1	genus					1637:1641	the genus Paenibacillus	1633:1655	the genus Paenibacillus	1633:1655	On the basis of the above results strain SYSU G01001T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus tepidiphilus sp.
31985395	2	51	theme	diversity	138:146	arg1	analysis					148:155	microbial diversity analysis	128:155	microbial diversity analysis of Tengchong hot spring, Yunnan province, south-west PR China	128:217	China is a hotspot for hot springs and during microbial diversity analysis of Tengchong hot spring, Yunnan province, south-west PR China, two strains designated SYSU G01001T and SY-13 were isolated.
31985395	12	52	theme	novel	1616:1620	arg1	species					1622:1628	a novel species	1614:1628	a novel species	1614:1628	On the basis of the above results strain SYSU G01001T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus tepidiphilus sp.
31985395	12	53	theme	name	1672:1675	arg1	sp					1704:1705	the name Paenibacillus tepidiphilus sp	1668:1705	the name Paenibacillus tepidiphilus sp	1668:1705	On the basis of the above results strain SYSU G01001T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus tepidiphilus sp.
31985395	9	54	theme	polar	1236:1240	arg1	L					1249:1249	L	1249:1249	L	1249:1249	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two unidentified aminolipids (AL), two unidentified amino phospholipids (APL), an unidentified phospholipid (PL) and an unidentified polar lipid (L).
31985395	9	54	theme	polar	1236:1240	arg1	lipid					1242:1246	an unidentified polar lipid	1220:1246	an unidentified polar lipid (L)	1220:1250	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two unidentified aminolipids (AL), two unidentified amino phospholipids (APL), an unidentified phospholipid (PL) and an unidentified polar lipid (L).
31985395	12	55	theme	genus	1637:1641	arg1	species					1622:1628	a novel species	1614:1628	a novel species	1614:1628	On the basis of the above results strain SYSU G01001T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus tepidiphilus sp.
31985395	2	56	theme	Yunnan	182:187	arg1	spring					174:179	Tengchong hot spring	160:179	Tengchong hot spring	160:179	China is a hotspot for hot springs and during microbial diversity analysis of Tengchong hot spring, Yunnan province, south-west PR China, two strains designated SYSU G01001T and SY-13 were isolated.
31985395	2	56	theme	Yunnan	182:187	arg1	province					189:196	Yunnan province	182:196	Yunnan province	182:196	China is a hotspot for hot springs and during microbial diversity analysis of Tengchong hot spring, Yunnan province, south-west PR China, two strains designated SYSU G01001T and SY-13 were isolated.
31985395	2	56	theme	Yunnan	182:187	arg1	China					213:217	south-west PR China	199:217	south-west PR China	199:217	China is a hotspot for hot springs and during microbial diversity analysis of Tengchong hot spring, Yunnan province, south-west PR China, two strains designated SYSU G01001T and SY-13 were isolated.
31985395	5	57	theme	optimum	486:492	arg1	23-37 °C					476:483	23-37 °C	476:483	23-37 °C (optimum 28 °C)	476:499	SYSU G01001T and SY-13 grew at pH 6.0-9.0 (optimum pH 8.0) and at 23-37 °C (optimum 28 °C).
31985395	5	57	theme	optimum	486:492	arg1	28 °C					494:498	optimum 28 °C	486:498	optimum 28 °C	486:498	SYSU G01001T and SY-13 grew at pH 6.0-9.0 (optimum pH 8.0) and at 23-37 °C (optimum 28 °C).
31985395	11	58	theme	identity	1326:1333	arg1	values					1351:1356	The average nucleotide identity (ANIb and ANIm) values	1303:1356	The average nucleotide identity (ANIb and ANIm) values between SYSU G01001T and Paenibacillus azotifigens LMG 29963T	1303:1418	The average nucleotide identity (ANIb and ANIm) values between SYSU G01001T and Paenibacillus azotifigens LMG 29963T were below the cut-off level (95-96 %) recommended as the average nucleotide identity (ANI) criterion for interspecies identity.
31985395	11	58	theme	identity	1326:1333	arg1	criterion					1512:1520	the average nucleotide identity (ANI) criterion	1474:1520	the average nucleotide identity (ANI) criterion for interspecies identity	1474:1546	The average nucleotide identity (ANIb and ANIm) values between SYSU G01001T and Paenibacillus azotifigens LMG 29963T were below the cut-off level (95-96 %) recommended as the average nucleotide identity (ANI) criterion for interspecies identity.
31985395	11	59	theme	Paenibacillus	1383:1395	arg1	azotifigens					1397:1407	Paenibacillus azotifigens	1383:1407	Paenibacillus azotifigens	1383:1407	The average nucleotide identity (ANIb and ANIm) values between SYSU G01001T and Paenibacillus azotifigens LMG 29963T were below the cut-off level (95-96 %) recommended as the average nucleotide identity (ANI) criterion for interspecies identity.
31985395	14	60	theme	SYSU	1745:1748	arg1	1.13870T					1778:1785	=KCTC 33952T=CGMCC 1.13870T	1759:1785	=KCTC 33952T=CGMCC 1.13870T	1759:1785	The type strain is SYSU G01001T (=KCTC 33952T=CGMCC 1.13870T).
31985395	14	60	theme	SYSU	1745:1748	arg1	strain					1735:1740	The type strain	1726:1740	The type strain	1726:1740	The type strain is SYSU G01001T (=KCTC 33952T=CGMCC 1.13870T).
31985395	14	60	theme	SYSU	1745:1748	arg1	G01001T					1750:1756	SYSU G01001T	1745:1756	SYSU G01001T (=KCTC 33952T=CGMCC 1.13870T)	1745:1786	The type strain is SYSU G01001T (=KCTC 33952T=CGMCC 1.13870T).
31985395	11	61	theme	interspecies	1526:1537	arg1	identity					1539:1546	interspecies identity	1526:1546	interspecies identity	1526:1546	The average nucleotide identity (ANIb and ANIm) values between SYSU G01001T and Paenibacillus azotifigens LMG 29963T were below the cut-off level (95-96 %) recommended as the average nucleotide identity (ANI) criterion for interspecies identity.
31985395	2	62	theme	spring	174:179	arg1	analysis					148:155	microbial diversity analysis	128:155	microbial diversity analysis of Tengchong hot spring, Yunnan province, south-west PR China	128:217	China is a hotspot for hot springs and during microbial diversity analysis of Tengchong hot spring, Yunnan province, south-west PR China, two strains designated SYSU G01001T and SY-13 were isolated.
31985395	11	63	theme	average	1307:1313	arg1	identity					1326:1333	average nucleotide identity	1307:1333	The average nucleotide identity (ANIb and ANIm) values between SYSU G01001T and Paenibacillus azotifigens LMG 29963T	1303:1418	The average nucleotide identity (ANIb and ANIm) values between SYSU G01001T and Paenibacillus azotifigens LMG 29963T were below the cut-off level (95-96 %) recommended as the average nucleotide identity (ANI) criterion for interspecies identity.
31985395	6	64	theme	sequence	611:618	arg1	similarity					620:629	low sequence similarity	607:629	low sequence similarity with Paenibacillus azotifigens (97.5 %)	607:669	The 16S rRNA gene sequence similarity between SYSU G01001T and SY-13 was 99.6 % but these strains shared low sequence similarity with Paenibacillus azotifigens (97.5 %) indicating that they represented a novel species.
31985395	2	65	theme	hot	170:172	arg1	spring					174:179	Tengchong hot spring	160:179	Tengchong hot spring	160:179	China is a hotspot for hot springs and during microbial diversity analysis of Tengchong hot spring, Yunnan province, south-west PR China, two strains designated SYSU G01001T and SY-13 were isolated.
31985395	2	65	theme	hot	170:172	arg1	province					189:196	Yunnan province	182:196	Yunnan province	182:196	China is a hotspot for hot springs and during microbial diversity analysis of Tengchong hot spring, Yunnan province, south-west PR China, two strains designated SYSU G01001T and SY-13 were isolated.
31985395	11	66	theme	nucleotide	1315:1324	arg1	identity					1326:1333	average nucleotide identity	1307:1333	The average nucleotide identity (ANIb and ANIm) values between SYSU G01001T and Paenibacillus azotifigens LMG 29963T	1303:1418	The average nucleotide identity (ANIb and ANIm) values between SYSU G01001T and Paenibacillus azotifigens LMG 29963T were below the cut-off level (95-96 %) recommended as the average nucleotide identity (ANI) criterion for interspecies identity.
31985395	9	67	dep	aminolipids	1120:1130	arg1	AL					1133:1134	AL	1133:1134	AL	1133:1134	The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two unidentified aminolipids (AL), two unidentified amino phospholipids (APL), an unidentified phospholipid (PL) and an unidentified polar lipid (L).
31985395	2	68	theme	hot	105:107	arg1	springs					109:115	hot springs	105:115	hot springs	105:115	China is a hotspot for hot springs and during microbial diversity analysis of Tengchong hot spring, Yunnan province, south-west PR China, two strains designated SYSU G01001T and SY-13 were isolated.
31985395	0	69	theme	Paenibacillus	15:27	arg1	sp					42:43	Paenibacillus tepidiphilus sp	15:43	Paenibacillus tepidiphilus sp	15:43	Description of Paenibacillus tepidiphilus sp.
31985395	11	70	theme	cut-off	1435:1441	arg1	level					1443:1447	the cut-off level	1431:1447	the cut-off level (95-96 %)	1431:1457	The average nucleotide identity (ANIb and ANIm) values between SYSU G01001T and Paenibacillus azotifigens LMG 29963T were below the cut-off level (95-96 %) recommended as the average nucleotide identity (ANI) criterion for interspecies identity.
31985395	11	70	theme	cut-off	1435:1441	arg1	%					1456:1456	95-96 %	1450:1456	95-96 %	1450:1456	The average nucleotide identity (ANIb and ANIm) values between SYSU G01001T and Paenibacillus azotifigens LMG 29963T were below the cut-off level (95-96 %) recommended as the average nucleotide identity (ANI) criterion for interspecies identity.
31985395	7	71	theme	further	780:786	arg1	investigations					788:801	further investigations	780:801	further investigations	780:801	On the basis of the results, SYSU G01001T was selected for further investigations and SY-13 was considered to represent a second strain of the species.
31974310	4	0	theme	cell	889:892	arg1	enzymes					909:915	multiple cell wall-degrading enzymes	880:915	multiple cell wall-degrading enzymes	880:915	The plants also exhibit extensive cell wall remodeling associated with induction of multiple cell wall-degrading enzymes, a process which renders the corresponding biomass a substrate for growth of the cellulolytic thermophile Caldicellulosiruptor bescii lacking a functional pectinase gene cluster.
31974310	3	1	theme	reductase	717:725	arg1	function					691:698	function	691:698	function of cinnamoyl CoA reductase 1 (CCR1)	691:734	Arabidopsis thaliana lines with altered lignin through down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) or loss of function of cinnamoyl CoA reductase 1 (CCR1) express a suite of pathogenesis-related (PR) protein genes.
31974310	4	2	theme	enzymes	909:915	arg1	induction					867:875	induction	867:875	induction of multiple cell wall-degrading enzymes	867:915	The plants also exhibit extensive cell wall remodeling associated with induction of multiple cell wall-degrading enzymes, a process which renders the corresponding biomass a substrate for growth of the cellulolytic thermophile Caldicellulosiruptor bescii lacking a functional pectinase gene cluster.
31974310	4	2	theme	enzymes	909:915	arg1	process					920:926	a process	918:926	a process which renders the corresponding biomass a substrate for growth of the cellulolytic thermophile Caldicellulosiruptor bescii lacking a functional pectinase gene cluster	918:1093	The plants also exhibit extensive cell wall remodeling associated with induction of multiple cell wall-degrading enzymes, a process which renders the corresponding biomass a substrate for growth of the cellulolytic thermophile Caldicellulosiruptor bescii lacking a functional pectinase gene cluster.
31974310	3	3	theme	altered	564:570	arg1	lignin					572:577	altered lignin	564:577	altered lignin through down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) or loss of function of cinnamoyl CoA reductase 1 (CCR1)	564:734	Arabidopsis thaliana lines with altered lignin through down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) or loss of function of cinnamoyl CoA reductase 1 (CCR1) express a suite of pathogenesis-related (PR) protein genes.
31974310	1	4	theme	plant	172:176	arg1	components					188:197	engineering plant cell wall components	160:197	engineering plant cell wall components	160:197	There is considerable interest in engineering plant cell wall components, particularly lignin, to improve forage quality and biomass properties for processing to fuels and bioproducts.
31974310	1	4	theme	plant	172:176	arg1	lignin					213:218	lignin	213:218	lignin	213:218	There is considerable interest in engineering plant cell wall components, particularly lignin, to improve forage quality and biomass properties for processing to fuels and bioproducts.
31974310	0	5	with	stems	91:95	arg1	content					117:123	reduced lignin content	102:123	reduced lignin content	102:123	ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1) releases latent defense signals in stems with reduced lignin content.
31974310	1	6	theme	wall	183:186	arg1	components					188:197	engineering plant cell wall components	160:197	engineering plant cell wall components	160:197	There is considerable interest in engineering plant cell wall components, particularly lignin, to improve forage quality and biomass properties for processing to fuels and bioproducts.
31974310	1	6	theme	wall	183:186	arg1	lignin					213:218	lignin	213:218	lignin	213:218	There is considerable interest in engineering plant cell wall components, particularly lignin, to improve forage quality and biomass properties for processing to fuels and bioproducts.
31974310	6	7	theme	gene	1379:1382	arg1	POLYGALACTURONASE					1412:1428	the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1	1375:1430	the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1)	1375:1438	Genetic analysis shows that both in planta PR gene expression and release of elicitors are the result of ectopic expression in xylem of the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1), which is normally expressed during anther and silique dehiscence.
31974310	6	7	theme	gene	1379:1382	arg1	ADPG1					1433:1437	ADPG1	1433:1437	ADPG1	1433:1437	Genetic analysis shows that both in planta PR gene expression and release of elicitors are the result of ectopic expression in xylem of the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1), which is normally expressed during anther and silique dehiscence.
31974310	7	8	theme	cell	1673:1676	arg1	walls					1678:1682	plant cell walls	1667:1682	plant cell walls	1667:1682	These data highlight the importance of pectin in cell wall integrity and the value of lignin modification as a tool to interrogate the informational content of plant cell walls.
31974310	4	9	theme	biomass	960:966	arg1	substrate					970:978	the corresponding biomass a substrate	942:978	the corresponding biomass a substrate for growth of the cellulolytic thermophile Caldicellulosiruptor bescii lacking a functional pectinase gene cluster	942:1093	The plants also exhibit extensive cell wall remodeling associated with induction of multiple cell wall-degrading enzymes, a process which renders the corresponding biomass a substrate for growth of the cellulolytic thermophile Caldicellulosiruptor bescii lacking a functional pectinase gene cluster.
31974310	3	10	theme	genes	789:793	arg1	suite					746:750	a suite	744:750	a suite of pathogenesis-related (PR) protein genes	744:793	Arabidopsis thaliana lines with altered lignin through down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) or loss of function of cinnamoyl CoA reductase 1 (CCR1) express a suite of pathogenesis-related (PR) protein genes.
31974310	3	11	theme	shikimate/quinate	627:643	arg1	transferase					662:672	hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase	606:672	hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT)	606:678	Arabidopsis thaliana lines with altered lignin through down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) or loss of function of cinnamoyl CoA reductase 1 (CCR1) express a suite of pathogenesis-related (PR) protein genes.
31974310	3	11	theme	shikimate/quinate	627:643	arg1	HCT					675:677	HCT	675:677	HCT	675:677	Arabidopsis thaliana lines with altered lignin through down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) or loss of function of cinnamoyl CoA reductase 1 (CCR1) express a suite of pathogenesis-related (PR) protein genes.
31974310	7	12	from	importance	1532:1541	arg1	integrity					1566:1574	cell wall integrity	1556:1574	cell wall integrity	1556:1574	These data highlight the importance of pectin in cell wall integrity and the value of lignin modification as a tool to interrogate the informational content of plant cell walls.
31974310	6	13	theme	PR	1282:1283	arg1	expression					1290:1299	planta PR gene expression	1275:1299	planta PR gene expression	1275:1299	Genetic analysis shows that both in planta PR gene expression and release of elicitors are the result of ectopic expression in xylem of the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1), which is normally expressed during anther and silique dehiscence.
31974310	3	14	theme	CoA	623:625	arg1	transferase					662:672	hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase	606:672	hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT)	606:678	Arabidopsis thaliana lines with altered lignin through down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) or loss of function of cinnamoyl CoA reductase 1 (CCR1) express a suite of pathogenesis-related (PR) protein genes.
31974310	3	14	theme	CoA	623:625	arg1	HCT					675:677	HCT	675:677	HCT	675:677	Arabidopsis thaliana lines with altered lignin through down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) or loss of function of cinnamoyl CoA reductase 1 (CCR1) express a suite of pathogenesis-related (PR) protein genes.
31974310	7	15	theme	informational	1642:1654	arg1	content					1656:1662	the informational content	1638:1662	the informational content of plant cell walls	1638:1682	These data highlight the importance of pectin in cell wall integrity and the value of lignin modification as a tool to interrogate the informational content of plant cell walls.
31974310	6	16	theme	ectopic	1344:1350	arg1	expression					1352:1361	ectopic expression	1344:1361	ectopic expression in xylem of the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1), which is normally expressed during anther and silique dehiscence	1344:1504	Genetic analysis shows that both in planta PR gene expression and release of elicitors are the result of ectopic expression in xylem of the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1), which is normally expressed during anther and silique dehiscence.
31974310	5	17	theme	cell	1100:1103	arg1	remodeling					1110:1119	The cell wall remodeling	1096:1119	The cell wall remodeling	1096:1119	The cell wall remodeling also results in the release of size- and charge-heterogeneous pectic oligosaccharide elicitors of PR gene expression.
31974310	3	18	theme	transferase	662:672	arg1	loss					683:686	loss	683:686	loss of function of cinnamoyl CoA reductase 1 (CCR1)	683:734	Arabidopsis thaliana lines with altered lignin through down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) or loss of function of cinnamoyl CoA reductase 1 (CCR1) express a suite of pathogenesis-related (PR) protein genes.
31974310	3	18	theme	transferase	662:672	arg1	down-regulation					587:601	down-regulation	587:601	down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT)	587:678	Arabidopsis thaliana lines with altered lignin through down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) or loss of function of cinnamoyl CoA reductase 1 (CCR1) express a suite of pathogenesis-related (PR) protein genes.
31974310	1	19	theme	considerable	135:146	arg1	interest					148:155	considerable interest	135:155	considerable interest in engineering plant cell wall components, particularly lignin, to improve forage quality and biomass properties for processing to fuels and bioproducts	135:308	There is considerable interest in engineering plant cell wall components, particularly lignin, to improve forage quality and biomass properties for processing to fuels and bioproducts.
31974310	4	20	theme	thermophile	1011:1021	arg1	bescii					1044:1049	the cellulolytic thermophile Caldicellulosiruptor bescii	994:1049	the cellulolytic thermophile Caldicellulosiruptor bescii lacking a functional pectinase gene cluster	994:1093	The plants also exhibit extensive cell wall remodeling associated with induction of multiple cell wall-degrading enzymes, a process which renders the corresponding biomass a substrate for growth of the cellulolytic thermophile Caldicellulosiruptor bescii lacking a functional pectinase gene cluster.
31974310	0	21	theme	defense	72:78	arg1	signals					80:86	latent defense signals	65:86	latent defense signals in stems with reduced lignin content	65:123	ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1) releases latent defense signals in stems with reduced lignin content.
31974310	5	22	theme	pectic	1183:1188	arg1	elicitors					1206:1214	size- and charge-heterogeneous pectic oligosaccharide elicitors	1152:1214	size- and charge-heterogeneous pectic oligosaccharide elicitors of PR gene expression	1152:1236	The cell wall remodeling also results in the release of size- and charge-heterogeneous pectic oligosaccharide elicitors of PR gene expression.
31974310	1	23	from	interest	148:155	arg1	components					188:197	engineering plant cell wall components	160:197	engineering plant cell wall components	160:197	There is considerable interest in engineering plant cell wall components, particularly lignin, to improve forage quality and biomass properties for processing to fuels and bioproducts.
31974310	1	23	from	interest	148:155	arg1	lignin					213:218	lignin	213:218	lignin	213:218	There is considerable interest in engineering plant cell wall components, particularly lignin, to improve forage quality and biomass properties for processing to fuels and bioproducts.
31974310	6	24	theme	POLYGALACTURONASE	1412:1428	arg1	xylem					1366:1370	xylem	1366:1370	xylem of the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1), which is normally expressed during anther and silique dehiscence	1366:1504	Genetic analysis shows that both in planta PR gene expression and release of elicitors are the result of ectopic expression in xylem of the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1), which is normally expressed during anther and silique dehiscence.
31974310	7	25	theme	lignin	1593:1598	arg1	modification					1600:1611	lignin modification	1593:1611	lignin modification	1593:1611	These data highlight the importance of pectin in cell wall integrity and the value of lignin modification as a tool to interrogate the informational content of plant cell walls.
31974310	2	26	theme	stress	524:529	arg1	absence					495:501	the absence	491:501	the absence of biotic or abiotic stress	491:529	However, modifying lignin content and/or composition in transgenic plants through down-regulation of lignin biosynthetic enzymes can induce expression of defense response genes in the absence of biotic or abiotic stress.
31974310	6	27	theme	DEHISCENCE	1396:1405	arg1	POLYGALACTURONASE					1412:1428	the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1	1375:1430	the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1)	1375:1438	Genetic analysis shows that both in planta PR gene expression and release of elicitors are the result of ectopic expression in xylem of the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1), which is normally expressed during anther and silique dehiscence.
31974310	6	27	theme	DEHISCENCE	1396:1405	arg1	ADPG1					1433:1437	ADPG1	1433:1437	ADPG1	1433:1437	Genetic analysis shows that both in planta PR gene expression and release of elicitors are the result of ectopic expression in xylem of the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1), which is normally expressed during anther and silique dehiscence.
31974310	2	28	theme	defense	465:471	arg1	genes					482:486	defense response genes	465:486	defense response genes	465:486	However, modifying lignin content and/or composition in transgenic plants through down-regulation of lignin biosynthetic enzymes can induce expression of defense response genes in the absence of biotic or abiotic stress.
31974310	4	29	theme	pectinase	1072:1080	arg1	cluster					1087:1093	a functional pectinase gene cluster	1059:1093	a functional pectinase gene cluster	1059:1093	The plants also exhibit extensive cell wall remodeling associated with induction of multiple cell wall-degrading enzymes, a process which renders the corresponding biomass a substrate for growth of the cellulolytic thermophile Caldicellulosiruptor bescii lacking a functional pectinase gene cluster.
31974310	0	30	theme	ARABIDOPSIS	0:10	arg1	POLYGALACTURONASE					28:44	ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1	0:46	ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1)	0:54	ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1) releases latent defense signals in stems with reduced lignin content.
31974310	0	30	theme	ARABIDOPSIS	0:10	arg1	ADPG1					49:53	ADPG1	49:53	ADPG1	49:53	ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1) releases latent defense signals in stems with reduced lignin content.
31974310	5	31	theme	expression	1227:1236	arg1	elicitors					1206:1214	size- and charge-heterogeneous pectic oligosaccharide elicitors	1152:1214	size- and charge-heterogeneous pectic oligosaccharide elicitors of PR gene expression	1152:1236	The cell wall remodeling also results in the release of size- and charge-heterogeneous pectic oligosaccharide elicitors of PR gene expression.
31974310	0	32	theme	ZONE	23:26	arg1	POLYGALACTURONASE					28:44	ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1	0:46	ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1)	0:54	ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1) releases latent defense signals in stems with reduced lignin content.
31974310	0	32	theme	ZONE	23:26	arg1	ADPG1					49:53	ADPG1	49:53	ADPG1	49:53	ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1) releases latent defense signals in stems with reduced lignin content.
31974310	4	33	theme	bescii	1044:1049	arg1	growth					984:989	growth	984:989	growth of the cellulolytic thermophile Caldicellulosiruptor bescii lacking a functional pectinase gene cluster	984:1093	The plants also exhibit extensive cell wall remodeling associated with induction of multiple cell wall-degrading enzymes, a process which renders the corresponding biomass a substrate for growth of the cellulolytic thermophile Caldicellulosiruptor bescii lacking a functional pectinase gene cluster.
31974310	4	34	theme	cell	830:833	arg1	remodeling					840:849	extensive cell wall remodeling	820:849	extensive cell wall remodeling associated with induction of multiple cell wall-degrading enzymes, a process which renders the corresponding biomass a substrate for growth of the cellulolytic thermophile Caldicellulosiruptor bescii lacking a functional pectinase gene cluster	820:1093	The plants also exhibit extensive cell wall remodeling associated with induction of multiple cell wall-degrading enzymes, a process which renders the corresponding biomass a substrate for growth of the cellulolytic thermophile Caldicellulosiruptor bescii lacking a functional pectinase gene cluster.
31974310	2	35	theme	biosynthetic	419:430	arg1	enzymes					432:438	lignin biosynthetic enzymes	412:438	lignin biosynthetic enzymes	412:438	However, modifying lignin content and/or composition in transgenic plants through down-regulation of lignin biosynthetic enzymes can induce expression of defense response genes in the absence of biotic or abiotic stress.
31974310	3	36	with	lines	553:557	arg1	lignin					572:577	altered lignin	564:577	altered lignin through down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) or loss of function of cinnamoyl CoA reductase 1 (CCR1)	564:734	Arabidopsis thaliana lines with altered lignin through down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) or loss of function of cinnamoyl CoA reductase 1 (CCR1) express a suite of pathogenesis-related (PR) protein genes.
31974310	5	37	theme	elicitors	1206:1214	arg1	release					1141:1147	the release	1137:1147	the release of size- and charge-heterogeneous pectic oligosaccharide elicitors of PR gene expression	1137:1236	The cell wall remodeling also results in the release of size- and charge-heterogeneous pectic oligosaccharide elicitors of PR gene expression.
31974310	2	38	theme	genes	482:486	arg1	expression					451:460	expression	451:460	expression of defense response genes	451:486	However, modifying lignin content and/or composition in transgenic plants through down-regulation of lignin biosynthetic enzymes can induce expression of defense response genes in the absence of biotic or abiotic stress.
31974310	6	39	from	expression	1352:1361	arg1	xylem					1366:1370	xylem	1366:1370	xylem of the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1), which is normally expressed during anther and silique dehiscence	1366:1504	Genetic analysis shows that both in planta PR gene expression and release of elicitors are the result of ectopic expression in xylem of the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1), which is normally expressed during anther and silique dehiscence.
31974310	5	40	theme	PR	1219:1220	arg1	expression					1227:1236	PR gene expression	1219:1236	PR gene expression	1219:1236	The cell wall remodeling also results in the release of size- and charge-heterogeneous pectic oligosaccharide elicitors of PR gene expression.
31974310	7	41	theme	cell	1556:1559	arg1	integrity					1566:1574	cell wall integrity	1556:1574	cell wall integrity	1556:1574	These data highlight the importance of pectin in cell wall integrity and the value of lignin modification as a tool to interrogate the informational content of plant cell walls.
31974310	3	42	theme	CoA	713:715	arg1	reductase					717:725	cinnamoyl CoA reductase 1	703:727	cinnamoyl CoA reductase 1 (CCR1)	703:734	Arabidopsis thaliana lines with altered lignin through down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) or loss of function of cinnamoyl CoA reductase 1 (CCR1) express a suite of pathogenesis-related (PR) protein genes.
31974310	3	42	theme	CoA	713:715	arg1	CCR1					730:733	CCR1	730:733	CCR1	730:733	Arabidopsis thaliana lines with altered lignin through down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) or loss of function of cinnamoyl CoA reductase 1 (CCR1) express a suite of pathogenesis-related (PR) protein genes.
31974310	7	43	theme	pectin	1546:1551	arg1	tool					1618:1621	a tool	1616:1621	a tool to interrogate the informational content of plant cell walls	1616:1682	These data highlight the importance of pectin in cell wall integrity and the value of lignin modification as a tool to interrogate the informational content of plant cell walls.
31974310	7	43	theme	pectin	1546:1551	arg1	importance					1532:1541	the importance	1528:1541	the importance of pectin in cell wall integrity	1528:1574	These data highlight the importance of pectin in cell wall integrity and the value of lignin modification as a tool to interrogate the informational content of plant cell walls.
31974310	7	43	theme	pectin	1546:1551	arg1	value					1584:1588	the value	1580:1588	the value of lignin modification	1580:1611	These data highlight the importance of pectin in cell wall integrity and the value of lignin modification as a tool to interrogate the informational content of plant cell walls.
31974310	2	44	theme	transgenic	367:376	arg1	plants					378:383	transgenic plants	367:383	transgenic plants	367:383	However, modifying lignin content and/or composition in transgenic plants through down-regulation of lignin biosynthetic enzymes can induce expression of defense response genes in the absence of biotic or abiotic stress.
31974310	4	45	theme	multiple	880:887	arg1	enzymes					909:915	multiple cell wall-degrading enzymes	880:915	multiple cell wall-degrading enzymes	880:915	The plants also exhibit extensive cell wall remodeling associated with induction of multiple cell wall-degrading enzymes, a process which renders the corresponding biomass a substrate for growth of the cellulolytic thermophile Caldicellulosiruptor bescii lacking a functional pectinase gene cluster.
31974310	1	46	theme	biomass	251:257	arg1	properties					259:268	biomass properties	251:268	biomass properties	251:268	There is considerable interest in engineering plant cell wall components, particularly lignin, to improve forage quality and biomass properties for processing to fuels and bioproducts.
31974310	4	47	theme	wall-degrading	894:907	arg1	enzymes					909:915	multiple cell wall-degrading enzymes	880:915	multiple cell wall-degrading enzymes	880:915	The plants also exhibit extensive cell wall remodeling associated with induction of multiple cell wall-degrading enzymes, a process which renders the corresponding biomass a substrate for growth of the cellulolytic thermophile Caldicellulosiruptor bescii lacking a functional pectinase gene cluster.
31974310	2	48	from	content	337:343	arg1	plants					378:383	transgenic plants	367:383	transgenic plants	367:383	However, modifying lignin content and/or composition in transgenic plants through down-regulation of lignin biosynthetic enzymes can induce expression of defense response genes in the absence of biotic or abiotic stress.
31974310	6	49	theme	Genetic	1239:1245	arg1	analysis					1247:1254	Genetic analysis	1239:1254	Genetic analysis	1239:1254	Genetic analysis shows that both in planta PR gene expression and release of elicitors are the result of ectopic expression in xylem of the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1), which is normally expressed during anther and silique dehiscence.
31974310	6	50	theme	elicitors	1316:1324	arg1	release					1305:1311	release	1305:1311	release of elicitors	1305:1324	Genetic analysis shows that both in planta PR gene expression and release of elicitors are the result of ectopic expression in xylem of the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1), which is normally expressed during anther and silique dehiscence.
31974310	6	50	theme	elicitors	1316:1324	arg1	expression					1290:1299	planta PR gene expression	1275:1299	planta PR gene expression	1275:1299	Genetic analysis shows that both in planta PR gene expression and release of elicitors are the result of ectopic expression in xylem of the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1), which is normally expressed during anther and silique dehiscence.
31974310	3	51	theme	thaliana	544:551	arg1	lines					553:557	Arabidopsis thaliana lines	532:557	Arabidopsis thaliana lines with altered lignin through down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) or loss of function of cinnamoyl CoA reductase 1 (CCR1)	532:734	Arabidopsis thaliana lines with altered lignin through down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) or loss of function of cinnamoyl CoA reductase 1 (CCR1) express a suite of pathogenesis-related (PR) protein genes.
31974310	1	52	theme	engineering	160:170	arg1	components					188:197	engineering plant cell wall components	160:197	engineering plant cell wall components	160:197	There is considerable interest in engineering plant cell wall components, particularly lignin, to improve forage quality and biomass properties for processing to fuels and bioproducts.
31974310	1	52	theme	engineering	160:170	arg1	lignin					213:218	lignin	213:218	lignin	213:218	There is considerable interest in engineering plant cell wall components, particularly lignin, to improve forage quality and biomass properties for processing to fuels and bioproducts.
31974310	7	53	from	value	1584:1588	arg1	integrity					1566:1574	cell wall integrity	1556:1574	cell wall integrity	1556:1574	These data highlight the importance of pectin in cell wall integrity and the value of lignin modification as a tool to interrogate the informational content of plant cell walls.
31974310	6	54	from	xylem	1366:1370	arg1	both					1267:1270	both	1267:1270	both	1267:1270	Genetic analysis shows that both in planta PR gene expression and release of elicitors are the result of ectopic expression in xylem of the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1), which is normally expressed during anther and silique dehiscence.
31974310	6	54	from	xylem	1366:1370	arg1	result					1334:1339	the result	1330:1339	the result of ectopic expression in xylem of the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1), which is normally expressed during anther and silique dehiscence	1330:1504	Genetic analysis shows that both in planta PR gene expression and release of elicitors are the result of ectopic expression in xylem of the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1), which is normally expressed during anther and silique dehiscence.
31974310	4	55	theme	cellulolytic	998:1009	arg1	bescii					1044:1049	the cellulolytic thermophile Caldicellulosiruptor bescii	994:1049	the cellulolytic thermophile Caldicellulosiruptor bescii lacking a functional pectinase gene cluster	994:1093	The plants also exhibit extensive cell wall remodeling associated with induction of multiple cell wall-degrading enzymes, a process which renders the corresponding biomass a substrate for growth of the cellulolytic thermophile Caldicellulosiruptor bescii lacking a functional pectinase gene cluster.
31974310	1	56	theme	cell	178:181	arg1	components					188:197	engineering plant cell wall components	160:197	engineering plant cell wall components	160:197	There is considerable interest in engineering plant cell wall components, particularly lignin, to improve forage quality and biomass properties for processing to fuels and bioproducts.
31974310	1	56	theme	cell	178:181	arg1	lignin					213:218	lignin	213:218	lignin	213:218	There is considerable interest in engineering plant cell wall components, particularly lignin, to improve forage quality and biomass properties for processing to fuels and bioproducts.
31974310	7	57	theme	walls	1678:1682	arg1	content					1656:1662	the informational content	1638:1662	the informational content of plant cell walls	1638:1682	These data highlight the importance of pectin in cell wall integrity and the value of lignin modification as a tool to interrogate the informational content of plant cell walls.
31974310	3	58	theme	PR	777:778	arg1	genes					789:793	pathogenesis-related (PR) protein genes	755:793	pathogenesis-related (PR) protein genes	755:793	Arabidopsis thaliana lines with altered lignin through down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) or loss of function of cinnamoyl CoA reductase 1 (CCR1) express a suite of pathogenesis-related (PR) protein genes.
31974310	6	59	theme	ARABIDOPSIS	1384:1394	arg1	POLYGALACTURONASE					1412:1428	the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1	1375:1430	the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1)	1375:1438	Genetic analysis shows that both in planta PR gene expression and release of elicitors are the result of ectopic expression in xylem of the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1), which is normally expressed during anther and silique dehiscence.
31974310	6	59	theme	ARABIDOPSIS	1384:1394	arg1	ADPG1					1433:1437	ADPG1	1433:1437	ADPG1	1433:1437	Genetic analysis shows that both in planta PR gene expression and release of elicitors are the result of ectopic expression in xylem of the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1), which is normally expressed during anther and silique dehiscence.
31974310	7	60	theme	plant	1667:1671	arg1	walls					1678:1682	plant cell walls	1667:1682	plant cell walls	1667:1682	These data highlight the importance of pectin in cell wall integrity and the value of lignin modification as a tool to interrogate the informational content of plant cell walls.
31974310	0	61	from	signals	80:86	arg1	stems					91:95	stems	91:95	stems with reduced lignin content	91:123	ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1) releases latent defense signals in stems with reduced lignin content.
31974310	3	62	theme	pathogenesis-related	755:774	arg1	genes					789:793	pathogenesis-related (PR) protein genes	755:793	pathogenesis-related (PR) protein genes	755:793	Arabidopsis thaliana lines with altered lignin through down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) or loss of function of cinnamoyl CoA reductase 1 (CCR1) express a suite of pathogenesis-related (PR) protein genes.
31974310	4	63	theme	corresponding	946:958	arg1	substrate					970:978	the corresponding biomass a substrate	942:978	the corresponding biomass a substrate for growth of the cellulolytic thermophile Caldicellulosiruptor bescii lacking a functional pectinase gene cluster	942:1093	The plants also exhibit extensive cell wall remodeling associated with induction of multiple cell wall-degrading enzymes, a process which renders the corresponding biomass a substrate for growth of the cellulolytic thermophile Caldicellulosiruptor bescii lacking a functional pectinase gene cluster.
31974310	6	64	theme	gene	1285:1288	arg1	expression					1290:1299	planta PR gene expression	1275:1299	planta PR gene expression	1275:1299	Genetic analysis shows that both in planta PR gene expression and release of elicitors are the result of ectopic expression in xylem of the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1), which is normally expressed during anther and silique dehiscence.
31974310	3	65	theme	hydroxycinnamoyl	606:621	arg1	transferase					662:672	hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase	606:672	hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT)	606:678	Arabidopsis thaliana lines with altered lignin through down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) or loss of function of cinnamoyl CoA reductase 1 (CCR1) express a suite of pathogenesis-related (PR) protein genes.
31974310	3	65	theme	hydroxycinnamoyl	606:621	arg1	HCT					675:677	HCT	675:677	HCT	675:677	Arabidopsis thaliana lines with altered lignin through down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) or loss of function of cinnamoyl CoA reductase 1 (CCR1) express a suite of pathogenesis-related (PR) protein genes.
31974310	6	66	theme	planta	1275:1280	arg1	expression					1290:1299	planta PR gene expression	1275:1299	planta PR gene expression	1275:1299	Genetic analysis shows that both in planta PR gene expression and release of elicitors are the result of ectopic expression in xylem of the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1), which is normally expressed during anther and silique dehiscence.
31974310	3	67	theme	protein	781:787	arg1	genes					789:793	pathogenesis-related (PR) protein genes	755:793	pathogenesis-related (PR) protein genes	755:793	Arabidopsis thaliana lines with altered lignin through down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) or loss of function of cinnamoyl CoA reductase 1 (CCR1) express a suite of pathogenesis-related (PR) protein genes.
31974310	6	68	theme	expression	1352:1361	arg1	both					1267:1270	both	1267:1270	both	1267:1270	Genetic analysis shows that both in planta PR gene expression and release of elicitors are the result of ectopic expression in xylem of the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1), which is normally expressed during anther and silique dehiscence.
31974310	6	68	theme	expression	1352:1361	arg1	result					1334:1339	the result	1330:1339	the result of ectopic expression in xylem of the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1), which is normally expressed during anther and silique dehiscence	1330:1504	Genetic analysis shows that both in planta PR gene expression and release of elicitors are the result of ectopic expression in xylem of the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1), which is normally expressed during anther and silique dehiscence.
31974310	1	69	dep	quality	239:245	arg1	forage					232:237	forage	232:237	forage	232:237	There is considerable interest in engineering plant cell wall components, particularly lignin, to improve forage quality and biomass properties for processing to fuels and bioproducts.
31974310	0	70	theme	latent	65:70	arg1	signals					80:86	latent defense signals	65:86	latent defense signals in stems with reduced lignin content	65:123	ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1) releases latent defense signals in stems with reduced lignin content.
31974310	5	71	theme	wall	1105:1108	arg1	remodeling					1110:1119	The cell wall remodeling	1096:1119	The cell wall remodeling	1096:1119	The cell wall remodeling also results in the release of size- and charge-heterogeneous pectic oligosaccharide elicitors of PR gene expression.
31974310	3	72	theme	hydroxycinnamoyl	645:660	arg1	transferase					662:672	hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase	606:672	hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT)	606:678	Arabidopsis thaliana lines with altered lignin through down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) or loss of function of cinnamoyl CoA reductase 1 (CCR1) express a suite of pathogenesis-related (PR) protein genes.
31974310	3	72	theme	hydroxycinnamoyl	645:660	arg1	HCT					675:677	HCT	675:677	HCT	675:677	Arabidopsis thaliana lines with altered lignin through down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) or loss of function of cinnamoyl CoA reductase 1 (CCR1) express a suite of pathogenesis-related (PR) protein genes.
31974310	2	73	theme	biotic	506:511	arg1	stress					524:529	biotic or abiotic stress	506:529	biotic or abiotic stress	506:529	However, modifying lignin content and/or composition in transgenic plants through down-regulation of lignin biosynthetic enzymes can induce expression of defense response genes in the absence of biotic or abiotic stress.
31974310	5	74	theme	charge-heterogeneous	1162:1181	arg1	elicitors					1206:1214	size- and charge-heterogeneous pectic oligosaccharide elicitors	1152:1214	size- and charge-heterogeneous pectic oligosaccharide elicitors of PR gene expression	1152:1236	The cell wall remodeling also results in the release of size- and charge-heterogeneous pectic oligosaccharide elicitors of PR gene expression.
31974310	7	75	theme	modification	1600:1611	arg1	tool					1618:1621	a tool	1616:1621	a tool to interrogate the informational content of plant cell walls	1616:1682	These data highlight the importance of pectin in cell wall integrity and the value of lignin modification as a tool to interrogate the informational content of plant cell walls.
31974310	7	75	theme	modification	1600:1611	arg1	importance					1532:1541	the importance	1528:1541	the importance of pectin in cell wall integrity	1528:1574	These data highlight the importance of pectin in cell wall integrity and the value of lignin modification as a tool to interrogate the informational content of plant cell walls.
31974310	7	75	theme	modification	1600:1611	arg1	value					1584:1588	the value	1580:1588	the value of lignin modification	1580:1611	These data highlight the importance of pectin in cell wall integrity and the value of lignin modification as a tool to interrogate the informational content of plant cell walls.
31974310	0	76	theme	lignin	110:115	arg1	content					117:123	reduced lignin content	102:123	reduced lignin content	102:123	ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1) releases latent defense signals in stems with reduced lignin content.
31974310	5	77	theme	oligosaccharide	1190:1204	arg1	elicitors					1206:1214	size- and charge-heterogeneous pectic oligosaccharide elicitors	1152:1214	size- and charge-heterogeneous pectic oligosaccharide elicitors of PR gene expression	1152:1236	The cell wall remodeling also results in the release of size- and charge-heterogeneous pectic oligosaccharide elicitors of PR gene expression.
31974310	4	78	theme	a	968:968	arg1	substrate					970:978	the corresponding biomass a substrate	942:978	the corresponding biomass a substrate for growth of the cellulolytic thermophile Caldicellulosiruptor bescii lacking a functional pectinase gene cluster	942:1093	The plants also exhibit extensive cell wall remodeling associated with induction of multiple cell wall-degrading enzymes, a process which renders the corresponding biomass a substrate for growth of the cellulolytic thermophile Caldicellulosiruptor bescii lacking a functional pectinase gene cluster.
31974310	6	79	theme	ZONE	1407:1410	arg1	POLYGALACTURONASE					1412:1428	the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1	1375:1430	the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1)	1375:1438	Genetic analysis shows that both in planta PR gene expression and release of elicitors are the result of ectopic expression in xylem of the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1), which is normally expressed during anther and silique dehiscence.
31974310	6	79	theme	ZONE	1407:1410	arg1	ADPG1					1433:1437	ADPG1	1433:1437	ADPG1	1433:1437	Genetic analysis shows that both in planta PR gene expression and release of elicitors are the result of ectopic expression in xylem of the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1), which is normally expressed during anther and silique dehiscence.
31974310	2	80	theme	abiotic	516:522	arg1	stress					524:529	biotic or abiotic stress	506:529	biotic or abiotic stress	506:529	However, modifying lignin content and/or composition in transgenic plants through down-regulation of lignin biosynthetic enzymes can induce expression of defense response genes in the absence of biotic or abiotic stress.
31974310	0	81	theme	reduced	102:108	arg1	content					117:123	reduced lignin content	102:123	reduced lignin content	102:123	ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1) releases latent defense signals in stems with reduced lignin content.
31974310	6	82	theme	silique	1487:1493	arg1	dehiscence					1495:1504	silique dehiscence	1487:1504	silique dehiscence	1487:1504	Genetic analysis shows that both in planta PR gene expression and release of elicitors are the result of ectopic expression in xylem of the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1), which is normally expressed during anther and silique dehiscence.
31974310	5	83	theme	size-	1152:1156	arg1	elicitors					1206:1214	size- and charge-heterogeneous pectic oligosaccharide elicitors	1152:1214	size- and charge-heterogeneous pectic oligosaccharide elicitors of PR gene expression	1152:1236	The cell wall remodeling also results in the release of size- and charge-heterogeneous pectic oligosaccharide elicitors of PR gene expression.
31974310	4	84	theme	functional	1061:1070	arg1	cluster					1087:1093	a functional pectinase gene cluster	1059:1093	a functional pectinase gene cluster	1059:1093	The plants also exhibit extensive cell wall remodeling associated with induction of multiple cell wall-degrading enzymes, a process which renders the corresponding biomass a substrate for growth of the cellulolytic thermophile Caldicellulosiruptor bescii lacking a functional pectinase gene cluster.
31974310	4	85	theme	gene	1082:1085	arg1	cluster					1087:1093	a functional pectinase gene cluster	1059:1093	a functional pectinase gene cluster	1059:1093	The plants also exhibit extensive cell wall remodeling associated with induction of multiple cell wall-degrading enzymes, a process which renders the corresponding biomass a substrate for growth of the cellulolytic thermophile Caldicellulosiruptor bescii lacking a functional pectinase gene cluster.
31974310	2	86	theme	enzymes	432:438	arg1	down-regulation					393:407	down-regulation	393:407	down-regulation of lignin biosynthetic enzymes	393:438	However, modifying lignin content and/or composition in transgenic plants through down-regulation of lignin biosynthetic enzymes can induce expression of defense response genes in the absence of biotic or abiotic stress.
31974310	4	87	theme	Caldicellulosiruptor	1023:1042	arg1	bescii					1044:1049	the cellulolytic thermophile Caldicellulosiruptor bescii	994:1049	the cellulolytic thermophile Caldicellulosiruptor bescii lacking a functional pectinase gene cluster	994:1093	The plants also exhibit extensive cell wall remodeling associated with induction of multiple cell wall-degrading enzymes, a process which renders the corresponding biomass a substrate for growth of the cellulolytic thermophile Caldicellulosiruptor bescii lacking a functional pectinase gene cluster.
31974310	4	88	theme	extensive	820:828	arg1	remodeling					840:849	extensive cell wall remodeling	820:849	extensive cell wall remodeling associated with induction of multiple cell wall-degrading enzymes, a process which renders the corresponding biomass a substrate for growth of the cellulolytic thermophile Caldicellulosiruptor bescii lacking a functional pectinase gene cluster	820:1093	The plants also exhibit extensive cell wall remodeling associated with induction of multiple cell wall-degrading enzymes, a process which renders the corresponding biomass a substrate for growth of the cellulolytic thermophile Caldicellulosiruptor bescii lacking a functional pectinase gene cluster.
31974310	2	89	theme	lignin	412:417	arg1	enzymes					432:438	lignin biosynthetic enzymes	412:438	lignin biosynthetic enzymes	412:438	However, modifying lignin content and/or composition in transgenic plants through down-regulation of lignin biosynthetic enzymes can induce expression of defense response genes in the absence of biotic or abiotic stress.
31974310	2	90	theme	response	473:480	arg1	genes					482:486	defense response genes	465:486	defense response genes	465:486	However, modifying lignin content and/or composition in transgenic plants through down-regulation of lignin biosynthetic enzymes can induce expression of defense response genes in the absence of biotic or abiotic stress.
31974310	3	91	theme	cinnamoyl	703:711	arg1	reductase					717:725	cinnamoyl CoA reductase 1	703:727	cinnamoyl CoA reductase 1 (CCR1)	703:734	Arabidopsis thaliana lines with altered lignin through down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) or loss of function of cinnamoyl CoA reductase 1 (CCR1) express a suite of pathogenesis-related (PR) protein genes.
31974310	3	91	theme	cinnamoyl	703:711	arg1	CCR1					730:733	CCR1	730:733	CCR1	730:733	Arabidopsis thaliana lines with altered lignin through down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) or loss of function of cinnamoyl CoA reductase 1 (CCR1) express a suite of pathogenesis-related (PR) protein genes.
31974310	4	92	theme	wall	835:838	arg1	remodeling					840:849	extensive cell wall remodeling	820:849	extensive cell wall remodeling associated with induction of multiple cell wall-degrading enzymes, a process which renders the corresponding biomass a substrate for growth of the cellulolytic thermophile Caldicellulosiruptor bescii lacking a functional pectinase gene cluster	820:1093	The plants also exhibit extensive cell wall remodeling associated with induction of multiple cell wall-degrading enzymes, a process which renders the corresponding biomass a substrate for growth of the cellulolytic thermophile Caldicellulosiruptor bescii lacking a functional pectinase gene cluster.
31974310	2	93	theme	lignin	330:335	arg1	content					337:343	lignin content	330:343	lignin content	330:343	However, modifying lignin content and/or composition in transgenic plants through down-regulation of lignin biosynthetic enzymes can induce expression of defense response genes in the absence of biotic or abiotic stress.
31974310	5	94	theme	gene	1222:1225	arg1	expression					1227:1236	PR gene expression	1219:1236	PR gene expression	1219:1236	The cell wall remodeling also results in the release of size- and charge-heterogeneous pectic oligosaccharide elicitors of PR gene expression.
31974310	7	95	theme	wall	1561:1564	arg1	integrity					1566:1574	cell wall integrity	1556:1574	cell wall integrity	1556:1574	These data highlight the importance of pectin in cell wall integrity and the value of lignin modification as a tool to interrogate the informational content of plant cell walls.
31974310	3	96	theme	function	691:698	arg1	loss					683:686	loss	683:686	loss of function of cinnamoyl CoA reductase 1 (CCR1)	683:734	Arabidopsis thaliana lines with altered lignin through down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) or loss of function of cinnamoyl CoA reductase 1 (CCR1) express a suite of pathogenesis-related (PR) protein genes.
31974310	3	96	theme	function	691:698	arg1	down-regulation					587:601	down-regulation	587:601	down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT)	587:678	Arabidopsis thaliana lines with altered lignin through down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) or loss of function of cinnamoyl CoA reductase 1 (CCR1) express a suite of pathogenesis-related (PR) protein genes.
31974310	3	97	theme	Arabidopsis	532:542	arg1	thaliana					544:551	Arabidopsis thaliana	532:551	Arabidopsis thaliana lines with altered lignin through down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) or loss of function of cinnamoyl CoA reductase 1 (CCR1)	532:734	Arabidopsis thaliana lines with altered lignin through down-regulation of hydroxycinnamoyl CoA:shikimate/quinate hydroxycinnamoyl transferase (HCT) or loss of function of cinnamoyl CoA reductase 1 (CCR1) express a suite of pathogenesis-related (PR) protein genes.
31974310	0	98	theme	DEHISCENCE	12:21	arg1	POLYGALACTURONASE					28:44	ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1	0:46	ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1)	0:54	ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1) releases latent defense signals in stems with reduced lignin content.
31974310	0	98	theme	DEHISCENCE	12:21	arg1	ADPG1					49:53	ADPG1	49:53	ADPG1	49:53	ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1) releases latent defense signals in stems with reduced lignin content.
31974310	6	99	from	result	1334:1339	arg1	xylem					1366:1370	xylem	1366:1370	xylem of the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1), which is normally expressed during anther and silique dehiscence	1366:1504	Genetic analysis shows that both in planta PR gene expression and release of elicitors are the result of ectopic expression in xylem of the gene ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 1 (ADPG1), which is normally expressed during anther and silique dehiscence.
32659425	3	0	from	proportion	686:695	arg1	matrix					712:717	the biofilm matrix	700:717	the biofilm matrix	700:717	Low concentrations of Cd2+ and montmorillonite or their combinations enhanced biofilm formation by increasing polysaccharides proportion in the biofilm matrix, and the maximum adsorption capacity of Cd2+ by biofilm was increased by 1.5 times.
32659425	1	1	theme	biofilm	166:172	arg1	interaction					141:151	The interaction	137:151	The interaction of bacterial biofilm and clay minerals	137:190	The interaction of bacterial biofilm and clay minerals provides great potential for heavy metal remediation in contaminated soil, yet, little is known about how heavy metal, clay minerals and their combinations affect the bacterial biofilm performance and heavy metal adsorption.
32659425	2	2	from	response	436:443	arg1	presence					502:509	the presence	498:509	the presence of Cd2+ and montmorillonite	498:537	In this study, the response of biofilm development as well as Cd2+ adsorption in the presence of Cd2+ and montmorillonite has been deciphered.
32659425	3	3	theme	biofilm	638:644	arg1	formation					646:654	biofilm formation	638:654	biofilm formation	638:654	Low concentrations of Cd2+ and montmorillonite or their combinations enhanced biofilm formation by increasing polysaccharides proportion in the biofilm matrix, and the maximum adsorption capacity of Cd2+ by biofilm was increased by 1.5 times.
32659425	5	4	from	tactics	974:980	arg1	systems					997:1003	the complex systems	985:1003	the complex systems which makes major contribution to microbial remediation of heavy metal polluted environments	985:1096	Such results could gain deeper insight into bacterial survival tactics in the complex systems which makes major contribution to microbial remediation of heavy metal polluted environments.
32659425	0	5	theme	matrix	103:108	arg1	changes					78:84	compositional changes	64:84	compositional changes of extracellular matrix in montmorillonite system	64:134	Synergistic effect of biofilm growth and cadmium adsorption via compositional changes of extracellular matrix in montmorillonite system.
32659425	3	6	theme	biofilm	704:710	arg1	matrix					712:717	the biofilm matrix	700:717	the biofilm matrix	700:717	Low concentrations of Cd2+ and montmorillonite or their combinations enhanced biofilm formation by increasing polysaccharides proportion in the biofilm matrix, and the maximum adsorption capacity of Cd2+ by biofilm was increased by 1.5 times.
32659425	0	7	theme	extracellular	89:101	arg1	matrix					103:108	extracellular matrix	89:108	extracellular matrix	89:108	Synergistic effect of biofilm growth and cadmium adsorption via compositional changes of extracellular matrix in montmorillonite system.
32659425	3	8	theme	maximum	728:734	arg1	capacity					747:754	the maximum adsorption capacity	724:754	the maximum adsorption capacity of Cd2+ by biofilm	724:773	Low concentrations of Cd2+ and montmorillonite or their combinations enhanced biofilm formation by increasing polysaccharides proportion in the biofilm matrix, and the maximum adsorption capacity of Cd2+ by biofilm was increased by 1.5 times.
32659425	5	9	theme	deeper	935:940	arg1	insight					942:948	deeper insight	935:948	deeper insight into bacterial survival tactics in the complex systems which makes major contribution to microbial remediation of heavy metal polluted environments	935:1096	Such results could gain deeper insight into bacterial survival tactics in the complex systems which makes major contribution to microbial remediation of heavy metal polluted environments.
32659425	0	10	theme	montmorillonite	113:127	arg1	system					129:134	montmorillonite system	113:134	montmorillonite system	113:134	Synergistic effect of biofilm growth and cadmium adsorption via compositional changes of extracellular matrix in montmorillonite system.
32659425	3	11	theme	adsorption	736:745	arg1	capacity					747:754	the maximum adsorption capacity	724:754	the maximum adsorption capacity of Cd2+ by biofilm	724:773	Low concentrations of Cd2+ and montmorillonite or their combinations enhanced biofilm formation by increasing polysaccharides proportion in the biofilm matrix, and the maximum adsorption capacity of Cd2+ by biofilm was increased by 1.5 times.
32659425	1	12	theme	clay	178:181	arg1	minerals					183:190	clay minerals	178:190	clay minerals	178:190	The interaction of bacterial biofilm and clay minerals provides great potential for heavy metal remediation in contaminated soil, yet, little is known about how heavy metal, clay minerals and their combinations affect the bacterial biofilm performance and heavy metal adsorption.
32659425	1	13	theme	bacterial	359:367	arg1	performance					377:387	the bacterial biofilm performance	355:387	the bacterial biofilm performance	355:387	The interaction of bacterial biofilm and clay minerals provides great potential for heavy metal remediation in contaminated soil, yet, little is known about how heavy metal, clay minerals and their combinations affect the bacterial biofilm performance and heavy metal adsorption.
32659425	4	14	theme	Cd2+	838:841	arg1	immobilization					820:833	the immobilization	816:833	the immobilization of Cd2+ by soil	816:849	Furthermore, the immobilization of Cd2+ by soil was significantly improved when S14-biofilm was introduced.
32659425	5	15	theme	complex	989:995	arg1	systems					997:1003	the complex systems	985:1003	the complex systems which makes major contribution to microbial remediation of heavy metal polluted environments	985:1096	Such results could gain deeper insight into bacterial survival tactics in the complex systems which makes major contribution to microbial remediation of heavy metal polluted environments.
32659425	1	16	theme	minerals	183:190	arg1	interaction					141:151	The interaction	137:151	The interaction of bacterial biofilm and clay minerals	137:190	The interaction of bacterial biofilm and clay minerals provides great potential for heavy metal remediation in contaminated soil, yet, little is known about how heavy metal, clay minerals and their combinations affect the bacterial biofilm performance and heavy metal adsorption.
32659425	1	17	theme	biofilm	369:375	arg1	performance					377:387	the bacterial biofilm performance	355:387	the bacterial biofilm performance	355:387	The interaction of bacterial biofilm and clay minerals provides great potential for heavy metal remediation in contaminated soil, yet, little is known about how heavy metal, clay minerals and their combinations affect the bacterial biofilm performance and heavy metal adsorption.
32659425	2	18	theme	adsorption	484:493	arg1	response					436:443	the response	432:443	the response of biofilm development as well as Cd2+ adsorption in the presence of Cd2+ and montmorillonite	432:537	In this study, the response of biofilm development as well as Cd2+ adsorption in the presence of Cd2+ and montmorillonite has been deciphered.
32659425	0	19	theme	Synergistic	0:10	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of biofilm growth and cadmium adsorption via compositional changes of extracellular matrix in montmorillonite system.	0:135	Synergistic effect of biofilm growth and cadmium adsorption via compositional changes of extracellular matrix in montmorillonite system.
32659425	3	20	theme	Cd2+	759:762	arg1	capacity					747:754	the maximum adsorption capacity	724:754	the maximum adsorption capacity of Cd2+ by biofilm	724:773	Low concentrations of Cd2+ and montmorillonite or their combinations enhanced biofilm formation by increasing polysaccharides proportion in the biofilm matrix, and the maximum adsorption capacity of Cd2+ by biofilm was increased by 1.5 times.
32659425	5	21	theme	polluted	1076:1083	arg1	environments					1085:1096	heavy metal polluted environments	1064:1096	heavy metal polluted environments	1064:1096	Such results could gain deeper insight into bacterial survival tactics in the complex systems which makes major contribution to microbial remediation of heavy metal polluted environments.
32659425	3	22	theme	Low	560:562	arg1	concentrations					564:577	Low concentrations	560:577	Low concentrations of Cd2+ and montmorillonite or their combinations	560:627	Low concentrations of Cd2+ and montmorillonite or their combinations enhanced biofilm formation by increasing polysaccharides proportion in the biofilm matrix, and the maximum adsorption capacity of Cd2+ by biofilm was increased by 1.5 times.
32659425	1	23	theme	great	201:205	arg1	potential					207:215	great potential	201:215	great potential for heavy metal remediation in contaminated soil	201:264	The interaction of bacterial biofilm and clay minerals provides great potential for heavy metal remediation in contaminated soil, yet, little is known about how heavy metal, clay minerals and their combinations affect the bacterial biofilm performance and heavy metal adsorption.
32659425	0	24	theme	biofilm	22:28	arg1	growth					30:35	biofilm growth	22:35	biofilm growth	22:35	Synergistic effect of biofilm growth and cadmium adsorption via compositional changes of extracellular matrix in montmorillonite system.
32659425	5	25	theme	bacterial	955:963	arg1	tactics					974:980	bacterial survival tactics	955:980	bacterial survival tactics in the complex systems which makes major contribution to microbial remediation of heavy metal polluted environments	955:1096	Such results could gain deeper insight into bacterial survival tactics in the complex systems which makes major contribution to microbial remediation of heavy metal polluted environments.
32659425	2	26	theme	montmorillonite	523:537	arg1	presence					502:509	the presence	498:509	the presence of Cd2+ and montmorillonite	498:537	In this study, the response of biofilm development as well as Cd2+ adsorption in the presence of Cd2+ and montmorillonite has been deciphered.
32659425	5	27	theme	environments	1085:1096	arg1	remediation					1049:1059	microbial remediation	1039:1059	microbial remediation of heavy metal polluted environments	1039:1096	Such results could gain deeper insight into bacterial survival tactics in the complex systems which makes major contribution to microbial remediation of heavy metal polluted environments.
32659425	5	28	theme	survival	965:972	arg1	tactics					974:980	bacterial survival tactics	955:980	bacterial survival tactics in the complex systems which makes major contribution to microbial remediation of heavy metal polluted environments	955:1096	Such results could gain deeper insight into bacterial survival tactics in the complex systems which makes major contribution to microbial remediation of heavy metal polluted environments.
32659425	0	29	from	changes	78:84	arg1	system					129:134	montmorillonite system	113:134	montmorillonite system	113:134	Synergistic effect of biofilm growth and cadmium adsorption via compositional changes of extracellular matrix in montmorillonite system.
32659425	3	30	theme	Cd2+	582:585	arg1	concentrations					564:577	Low concentrations	560:577	Low concentrations of Cd2+ and montmorillonite or their combinations	560:627	Low concentrations of Cd2+ and montmorillonite or their combinations enhanced biofilm formation by increasing polysaccharides proportion in the biofilm matrix, and the maximum adsorption capacity of Cd2+ by biofilm was increased by 1.5 times.
32659425	1	31	theme	heavy	221:225	arg1	remediation					233:243	heavy metal remediation	221:243	heavy metal remediation in contaminated soil	221:264	The interaction of bacterial biofilm and clay minerals provides great potential for heavy metal remediation in contaminated soil, yet, little is known about how heavy metal, clay minerals and their combinations affect the bacterial biofilm performance and heavy metal adsorption.
32659425	0	32	theme	growth	30:35	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of biofilm growth and cadmium adsorption via compositional changes of extracellular matrix in montmorillonite system.	0:135	Synergistic effect of biofilm growth and cadmium adsorption via compositional changes of extracellular matrix in montmorillonite system.
32659425	2	33	theme	development	456:466	arg1	response					436:443	the response	432:443	the response of biofilm development as well as Cd2+ adsorption in the presence of Cd2+ and montmorillonite	432:537	In this study, the response of biofilm development as well as Cd2+ adsorption in the presence of Cd2+ and montmorillonite has been deciphered.
32659425	1	34	theme	heavy	393:397	arg1	adsorption					405:414	heavy metal adsorption	393:414	heavy metal adsorption	393:414	The interaction of bacterial biofilm and clay minerals provides great potential for heavy metal remediation in contaminated soil, yet, little is known about how heavy metal, clay minerals and their combinations affect the bacterial biofilm performance and heavy metal adsorption.
32659425	2	35	theme	Cd2+	514:517	arg1	presence					502:509	the presence	498:509	the presence of Cd2+ and montmorillonite	498:537	In this study, the response of biofilm development as well as Cd2+ adsorption in the presence of Cd2+ and montmorillonite has been deciphered.
32659425	1	36	theme	metal	227:231	arg1	remediation					233:243	heavy metal remediation	221:243	heavy metal remediation in contaminated soil	221:264	The interaction of bacterial biofilm and clay minerals provides great potential for heavy metal remediation in contaminated soil, yet, little is known about how heavy metal, clay minerals and their combinations affect the bacterial biofilm performance and heavy metal adsorption.
32659425	0	37	theme	adsorption	49:58	arg1	effect					12:17	Synergistic effect	0:17	Synergistic effect of biofilm growth and cadmium adsorption via compositional changes of extracellular matrix in montmorillonite system.	0:135	Synergistic effect of biofilm growth and cadmium adsorption via compositional changes of extracellular matrix in montmorillonite system.
32659425	1	38	theme	metal	399:403	arg1	adsorption					405:414	heavy metal adsorption	393:414	heavy metal adsorption	393:414	The interaction of bacterial biofilm and clay minerals provides great potential for heavy metal remediation in contaminated soil, yet, little is known about how heavy metal, clay minerals and their combinations affect the bacterial biofilm performance and heavy metal adsorption.
32659425	5	39	theme	microbial	1039:1047	arg1	remediation					1049:1059	microbial remediation	1039:1059	microbial remediation of heavy metal polluted environments	1039:1096	Such results could gain deeper insight into bacterial survival tactics in the complex systems which makes major contribution to microbial remediation of heavy metal polluted environments.
32659425	0	40	theme	cadmium	41:47	arg1	adsorption					49:58	cadmium adsorption	41:58	cadmium adsorption	41:58	Synergistic effect of biofilm growth and cadmium adsorption via compositional changes of extracellular matrix in montmorillonite system.
32659425	1	41	theme	clay	311:314	arg1	minerals					316:323	clay minerals	311:323	clay minerals	311:323	The interaction of bacterial biofilm and clay minerals provides great potential for heavy metal remediation in contaminated soil, yet, little is known about how heavy metal, clay minerals and their combinations affect the bacterial biofilm performance and heavy metal adsorption.
32659425	1	42	from	remediation	233:243	arg1	soil					261:264	contaminated soil	248:264	contaminated soil	248:264	The interaction of bacterial biofilm and clay minerals provides great potential for heavy metal remediation in contaminated soil, yet, little is known about how heavy metal, clay minerals and their combinations affect the bacterial biofilm performance and heavy metal adsorption.
32659425	5	43	theme	heavy	1064:1068	arg1	environments					1085:1096	heavy metal polluted environments	1064:1096	heavy metal polluted environments	1064:1096	Such results could gain deeper insight into bacterial survival tactics in the complex systems which makes major contribution to microbial remediation of heavy metal polluted environments.
32659425	5	44	theme	Such	911:914	arg1	results					916:922	Such results	911:922	Such results	911:922	Such results could gain deeper insight into bacterial survival tactics in the complex systems which makes major contribution to microbial remediation of heavy metal polluted environments.
32659425	5	45	theme	metal	1070:1074	arg1	environments					1085:1096	heavy metal polluted environments	1064:1096	heavy metal polluted environments	1064:1096	Such results could gain deeper insight into bacterial survival tactics in the complex systems which makes major contribution to microbial remediation of heavy metal polluted environments.
32659425	3	46	theme	combinations	616:627	arg1	concentrations					564:577	Low concentrations	560:577	Low concentrations of Cd2+ and montmorillonite or their combinations	560:627	Low concentrations of Cd2+ and montmorillonite or their combinations enhanced biofilm formation by increasing polysaccharides proportion in the biofilm matrix, and the maximum adsorption capacity of Cd2+ by biofilm was increased by 1.5 times.
32659425	2	47	theme	Cd2+	479:482	arg1	adsorption					484:493	Cd2+ adsorption	479:493	biofilm development as well as Cd2+ adsorption	448:493	In this study, the response of biofilm development as well as Cd2+ adsorption in the presence of Cd2+ and montmorillonite has been deciphered.
32659425	0	48	theme	compositional	64:76	arg1	changes					78:84	compositional changes	64:84	compositional changes of extracellular matrix in montmorillonite system	64:134	Synergistic effect of biofilm growth and cadmium adsorption via compositional changes of extracellular matrix in montmorillonite system.
32659425	3	49	theme	polysaccharides	670:684	arg1	proportion					686:695	polysaccharides proportion	670:695	polysaccharides proportion in the biofilm matrix	670:717	Low concentrations of Cd2+ and montmorillonite or their combinations enhanced biofilm formation by increasing polysaccharides proportion in the biofilm matrix, and the maximum adsorption capacity of Cd2+ by biofilm was increased by 1.5 times.
32659425	3	50	theme	montmorillonite	591:605	arg1	concentrations					564:577	Low concentrations	560:577	Low concentrations of Cd2+ and montmorillonite or their combinations	560:627	Low concentrations of Cd2+ and montmorillonite or their combinations enhanced biofilm formation by increasing polysaccharides proportion in the biofilm matrix, and the maximum adsorption capacity of Cd2+ by biofilm was increased by 1.5 times.
32659425	1	51	theme	contaminated	248:259	arg1	soil					261:264	contaminated soil	248:264	contaminated soil	248:264	The interaction of bacterial biofilm and clay minerals provides great potential for heavy metal remediation in contaminated soil, yet, little is known about how heavy metal, clay minerals and their combinations affect the bacterial biofilm performance and heavy metal adsorption.
32659425	2	52	theme	biofilm	448:454	arg1	development					456:466	biofilm development	448:466	biofilm development as well as Cd2+ adsorption	448:493	In this study, the response of biofilm development as well as Cd2+ adsorption in the presence of Cd2+ and montmorillonite has been deciphered.
32659425	5	53	theme	major	1017:1021	arg1	contribution					1023:1034	major contribution	1017:1034	major contribution to microbial remediation of heavy metal polluted environments	1017:1096	Such results could gain deeper insight into bacterial survival tactics in the complex systems which makes major contribution to microbial remediation of heavy metal polluted environments.
32659425	1	54	theme	bacterial	156:164	arg1	biofilm					166:172	bacterial biofilm	156:172	bacterial biofilm	156:172	The interaction of bacterial biofilm and clay minerals provides great potential for heavy metal remediation in contaminated soil, yet, little is known about how heavy metal, clay minerals and their combinations affect the bacterial biofilm performance and heavy metal adsorption.
35008562	0	0	from	Coating	21:27	arg1	Ti6Al4V					32:38	Ti6Al4V	32:38	Ti6Al4V	32:38	Heparin Enriched-WPI Coating on Ti6Al4V Increases Hydrophilicity and Improves Proliferation and Differentiation of Human Bone Marrow Stromal Cells.
35008562	2	1	theme	bone	347:350	arg1	formation					352:360	bone formation	347:360	bone formation	347:360	The success of Ti6Al4V implants depends on bone formation on the implant surface.
35008562	7	2	theme	WPI	968:970	arg1	fibrils					972:978	WPI fibrils	968:978	WPI fibrils	968:978	Indeed, WPI fibrils were resistant to sterilization and were stable during storage.
35008562	6	3	theme	cells	945:949	arg1	growth					909:914	the growth	905:914	the growth of human bone marrow stromal cells (hBMSC)	905:957	WPI fibrils proved to be an excellent support for the growth of human bone marrow stromal cells (hBMSC).
35008562	11	4	theme	adsorption	1588:1597	arg1	procedure					1599:1607	a simple adsorption procedure	1579:1607	a simple adsorption procedure	1579:1607	Indeed, the already known positive effect on osteogenic integration of WPI-only coated substrates has been further enhanced by a simple adsorption procedure.
35008562	8	5	theme	non-specific	1159:1170	arg1	TNAP					1194:1197	TNAP	1194:1197	TNAP	1194:1197	This WPI-heparin-enriched coating, especially the LMWH, enhanced the differentiation of hBMSC by increasing tissue non-specific alkaline phosphatase (TNAP) activity.
35008562	8	5	theme	non-specific	1159:1170	arg1	phosphatase					1181:1191	tissue non-specific alkaline phosphatase	1152:1191	tissue non-specific alkaline phosphatase (TNAP) activity	1152:1207	This WPI-heparin-enriched coating, especially the LMWH, enhanced the differentiation of hBMSC by increasing tissue non-specific alkaline phosphatase (TNAP) activity.
35008562	10	6	theme	biomedical	1367:1376	arg1	coatings					1378:1385	biomedical coatings	1367:1385	biomedical coatings	1367:1385	The results confirmed that WPI fibrils are an excellent biomaterial which can be used for biomedical coatings, as they are easily modifiable and resistant to heat treatments.
35008562	6	7	theme	bone	925:928	arg1	hBMSC					952:956	hBMSC	952:956	hBMSC	952:956	WPI fibrils proved to be an excellent support for the growth of human bone marrow stromal cells (hBMSC).
35008562	6	7	theme	bone	925:928	arg1	cells					945:949	human bone marrow stromal cells	919:949	human bone marrow stromal cells (hBMSC)	919:957	WPI fibrils proved to be an excellent support for the growth of human bone marrow stromal cells (hBMSC).
35008562	6	8	theme	stromal	937:943	arg1	hBMSC					952:956	hBMSC	952:956	hBMSC	952:956	WPI fibrils proved to be an excellent support for the growth of human bone marrow stromal cells (hBMSC).
35008562	6	8	theme	stromal	937:943	arg1	cells					945:949	human bone marrow stromal cells	919:949	human bone marrow stromal cells (hBMSC)	919:957	WPI fibrils proved to be an excellent support for the growth of human bone marrow stromal cells (hBMSC).
35008562	0	9	theme	Human	115:119	arg1	Cells					141:145	Human Bone Marrow Stromal Cells	115:145	Human Bone Marrow Stromal Cells	115:145	Heparin Enriched-WPI Coating on Ti6Al4V Increases Hydrophilicity and Improves Proliferation and Differentiation of Human Bone Marrow Stromal Cells.
35008562	4	10	theme	coating	509:515	arg1	strategy					517:524	One coating strategy	505:524	One coating strategy	505:524	One coating strategy is by adsorption of biomacromolecules.
35008562	5	11	theme	whey	656:659	arg1	fibrils					683:689	whey protein isolate (WPI) fibrils	656:689	whey protein isolate (WPI) fibrils	656:689	In this study, Ti6Al4V substrates produced by additive manufacturing (AM) were coated with whey protein isolate (WPI) fibrils, obtained at pH 2, and heparin or tinzaparin (a low molecular weight heparin LMWH) in order to improve the proliferation and differentiation of bone-forming cells.
35008562	10	12	theme	excellent	1323:1331	arg1	biomaterial					1333:1343	an excellent biomaterial	1320:1343	an excellent biomaterial which can be used for biomedical coatings	1320:1385	The results confirmed that WPI fibrils are an excellent biomaterial which can be used for biomedical coatings, as they are easily modifiable and resistant to heat treatments.
35008562	10	12	theme	excellent	1323:1331	arg1	fibrils					1308:1314	WPI fibrils	1304:1314	WPI fibrils	1304:1314	The results confirmed that WPI fibrils are an excellent biomaterial which can be used for biomedical coatings, as they are easily modifiable and resistant to heat treatments.
35008562	5	13	theme	cells	848:852	arg1	differentiation					816:830	differentiation	816:830	differentiation	816:830	In this study, Ti6Al4V substrates produced by additive manufacturing (AM) were coated with whey protein isolate (WPI) fibrils, obtained at pH 2, and heparin or tinzaparin (a low molecular weight heparin LMWH) in order to improve the proliferation and differentiation of bone-forming cells.
35008562	5	13	theme	cells	848:852	arg1	proliferation					798:810	the proliferation	794:810	the proliferation	794:810	In this study, Ti6Al4V substrates produced by additive manufacturing (AM) were coated with whey protein isolate (WPI) fibrils, obtained at pH 2, and heparin or tinzaparin (a low molecular weight heparin LMWH) in order to improve the proliferation and differentiation of bone-forming cells.
35008562	6	14	theme	marrow	930:935	arg1	hBMSC					952:956	hBMSC	952:956	hBMSC	952:956	WPI fibrils proved to be an excellent support for the growth of human bone marrow stromal cells (hBMSC).
35008562	6	14	theme	marrow	930:935	arg1	cells					945:949	human bone marrow stromal cells	919:949	human bone marrow stromal cells (hBMSC)	919:957	WPI fibrils proved to be an excellent support for the growth of human bone marrow stromal cells (hBMSC).
35008562	6	15	theme	WPI	855:857	arg1	support					893:899	an excellent support	880:899	an excellent support for the growth of human bone marrow stromal cells (hBMSC)	880:957	WPI fibrils proved to be an excellent support for the growth of human bone marrow stromal cells (hBMSC).
35008562	6	15	theme	WPI	855:857	arg1	fibrils					859:865	WPI fibrils	855:865	WPI fibrils	855:865	WPI fibrils proved to be an excellent support for the growth of human bone marrow stromal cells (hBMSC).
35008562	5	16	theme	protein	661:667	arg1	fibrils					683:689	whey protein isolate (WPI) fibrils	656:689	whey protein isolate (WPI) fibrils	656:689	In this study, Ti6Al4V substrates produced by additive manufacturing (AM) were coated with whey protein isolate (WPI) fibrils, obtained at pH 2, and heparin or tinzaparin (a low molecular weight heparin LMWH) in order to improve the proliferation and differentiation of bone-forming cells.
35008562	5	17	theme	additive	611:618	arg1	AM					635:636	AM	635:636	AM	635:636	In this study, Ti6Al4V substrates produced by additive manufacturing (AM) were coated with whey protein isolate (WPI) fibrils, obtained at pH 2, and heparin or tinzaparin (a low molecular weight heparin LMWH) in order to improve the proliferation and differentiation of bone-forming cells.
35008562	5	17	theme	additive	611:618	arg1	manufacturing					620:632	additive manufacturing	611:632	additive manufacturing (AM)	611:637	In this study, Ti6Al4V substrates produced by additive manufacturing (AM) were coated with whey protein isolate (WPI) fibrils, obtained at pH 2, and heparin or tinzaparin (a low molecular weight heparin LMWH) in order to improve the proliferation and differentiation of bone-forming cells.
35008562	3	18	theme	cells	484:488	arg1	adhesion					424:431	adhesion	424:431	adhesion	424:431	Hence, implant coatings which promote adhesion, proliferation and differentiation of bone-forming cells are desirable.
35008562	3	18	theme	cells	484:488	arg1	differentiation					452:466	differentiation	452:466	differentiation	452:466	Hence, implant coatings which promote adhesion, proliferation and differentiation of bone-forming cells are desirable.
35008562	3	18	theme	cells	484:488	arg1	proliferation					434:446	proliferation	434:446	proliferation	434:446	Hence, implant coatings which promote adhesion, proliferation and differentiation of bone-forming cells are desirable.
35008562	0	19	theme	Marrow	126:131	arg1	Cells					141:145	Human Bone Marrow Stromal Cells	115:145	Human Bone Marrow Stromal Cells	115:145	Heparin Enriched-WPI Coating on Ti6Al4V Increases Hydrophilicity and Improves Proliferation and Differentiation of Human Bone Marrow Stromal Cells.
35008562	11	20	theme	simple	1581:1586	arg1	procedure					1599:1607	a simple adsorption procedure	1579:1607	a simple adsorption procedure	1579:1607	Indeed, the already known positive effect on osteogenic integration of WPI-only coated substrates has been further enhanced by a simple adsorption procedure.
35008562	0	21	theme	Bone	121:124	arg1	Cells					141:145	Human Bone Marrow Stromal Cells	115:145	Human Bone Marrow Stromal Cells	115:145	Heparin Enriched-WPI Coating on Ti6Al4V Increases Hydrophilicity and Improves Proliferation and Differentiation of Human Bone Marrow Stromal Cells.
35008562	8	22	theme	tissue	1152:1157	arg1	TNAP					1194:1197	TNAP	1194:1197	TNAP	1194:1197	This WPI-heparin-enriched coating, especially the LMWH, enhanced the differentiation of hBMSC by increasing tissue non-specific alkaline phosphatase (TNAP) activity.
35008562	8	22	theme	tissue	1152:1157	arg1	phosphatase					1181:1191	tissue non-specific alkaline phosphatase	1152:1191	tissue non-specific alkaline phosphatase (TNAP) activity	1152:1207	This WPI-heparin-enriched coating, especially the LMWH, enhanced the differentiation of hBMSC by increasing tissue non-specific alkaline phosphatase (TNAP) activity.
35008562	11	23	theme	osteogenic	1497:1506	arg1	integration					1508:1518	osteogenic integration	1497:1518	osteogenic integration of WPI-only coated substrates	1497:1548	Indeed, the already known positive effect on osteogenic integration of WPI-only coated substrates has been further enhanced by a simple adsorption procedure.
35008562	10	24	used	used	1358:1361	arg2	fibrils					1308:1314	WPI fibrils	1304:1314	WPI fibrils	1304:1314	The results confirmed that WPI fibrils are an excellent biomaterial which can be used for biomedical coatings, as they are easily modifiable and resistant to heat treatments.
35008562	10	24	used	used	1358:1361	arg2	biomaterial					1333:1343	an excellent biomaterial	1320:1343	an excellent biomaterial which can be used for biomedical coatings	1320:1385	The results confirmed that WPI fibrils are an excellent biomaterial which can be used for biomedical coatings, as they are easily modifiable and resistant to heat treatments.
35008562	8	25	theme	phosphatase	1181:1191	arg1	activity					1200:1207	tissue non-specific alkaline phosphatase (TNAP) activity	1152:1207	tissue non-specific alkaline phosphatase (TNAP) activity	1152:1207	This WPI-heparin-enriched coating, especially the LMWH, enhanced the differentiation of hBMSC by increasing tissue non-specific alkaline phosphatase (TNAP) activity.
35008562	0	26	theme	Enriched-WPI	8:19	arg1	Coating					21:27	Heparin Enriched-WPI Coating	0:27	Heparin Enriched-WPI Coating on Ti6Al4V	0:38	Heparin Enriched-WPI Coating on Ti6Al4V Increases Hydrophilicity and Improves Proliferation and Differentiation of Human Bone Marrow Stromal Cells.
35008562	9	27	theme	material	1267:1274	arg1	hydrophilicity					1245:1258	the hydrophilicity	1241:1258	the hydrophilicity of the material	1241:1274	Finally, the coating increased the hydrophilicity of the material.
35008562	6	28	theme	human	919:923	arg1	hBMSC					952:956	hBMSC	952:956	hBMSC	952:956	WPI fibrils proved to be an excellent support for the growth of human bone marrow stromal cells (hBMSC).
35008562	6	28	theme	human	919:923	arg1	cells					945:949	human bone marrow stromal cells	919:949	human bone marrow stromal cells (hBMSC)	919:957	WPI fibrils proved to be an excellent support for the growth of human bone marrow stromal cells (hBMSC).
35008562	2	29	theme	implant	369:375	arg1	surface					377:383	the implant surface	365:383	the implant surface	365:383	The success of Ti6Al4V implants depends on bone formation on the implant surface.
35008562	0	30	theme	Heparin	0:6	arg1	Coating					21:27	Heparin Enriched-WPI Coating	0:27	Heparin Enriched-WPI Coating on Ti6Al4V	0:38	Heparin Enriched-WPI Coating on Ti6Al4V Increases Hydrophilicity and Improves Proliferation and Differentiation of Human Bone Marrow Stromal Cells.
35008562	1	31	theme	mechanical	263:272	arg1	loading					274:280	mechanical loading	263:280	mechanical loading	263:280	Titanium alloy (Ti6Al4V) is one of the most prominent biomaterials for bone contact because of its ability to bear mechanical loading and resist corrosion.
35008562	5	32	theme	Ti6Al4V	580:586	arg1	substrates					588:597	Ti6Al4V substrates	580:597	Ti6Al4V substrates produced by additive manufacturing (AM)	580:637	In this study, Ti6Al4V substrates produced by additive manufacturing (AM) were coated with whey protein isolate (WPI) fibrils, obtained at pH 2, and heparin or tinzaparin (a low molecular weight heparin LMWH) in order to improve the proliferation and differentiation of bone-forming cells.
35008562	4	33	theme	biomacromolecules	546:562	arg1	adsorption					532:541	adsorption	532:541	adsorption of biomacromolecules	532:562	One coating strategy is by adsorption of biomacromolecules.
35008562	0	34	theme	Cells	141:145	arg1	Differentiation					96:110	Differentiation	96:110	Differentiation	96:110	Heparin Enriched-WPI Coating on Ti6Al4V Increases Hydrophilicity and Improves Proliferation and Differentiation of Human Bone Marrow Stromal Cells.
35008562	0	34	theme	Cells	141:145	arg1	Proliferation					78:90	Proliferation	78:90	Proliferation	78:90	Heparin Enriched-WPI Coating on Ti6Al4V Increases Hydrophilicity and Improves Proliferation and Differentiation of Human Bone Marrow Stromal Cells.
35008562	11	35	theme	known	1472:1476	arg1	effect					1487:1492	the already known positive effect	1460:1492	the already known positive effect on osteogenic integration of WPI-only coated substrates	1460:1548	Indeed, the already known positive effect on osteogenic integration of WPI-only coated substrates has been further enhanced by a simple adsorption procedure.
35008562	8	36	theme	hBMSC	1132:1136	arg1	differentiation					1113:1127	the differentiation	1109:1127	the differentiation of hBMSC	1109:1136	This WPI-heparin-enriched coating, especially the LMWH, enhanced the differentiation of hBMSC by increasing tissue non-specific alkaline phosphatase (TNAP) activity.
35008562	0	37	theme	Stromal	133:139	arg1	Cells					141:145	Human Bone Marrow Stromal Cells	115:145	Human Bone Marrow Stromal Cells	115:145	Heparin Enriched-WPI Coating on Ti6Al4V Increases Hydrophilicity and Improves Proliferation and Differentiation of Human Bone Marrow Stromal Cells.
35008562	1	38	theme	prominent	192:200	arg1	biomaterials					202:213	the most prominent biomaterials	183:213	the most prominent biomaterials for bone contact	183:230	Titanium alloy (Ti6Al4V) is one of the most prominent biomaterials for bone contact because of its ability to bear mechanical loading and resist corrosion.
35008562	3	39	theme	implant	393:399	arg1	coatings					401:408	implant coatings	393:408	implant coatings which promote adhesion, proliferation and differentiation of bone-forming cells	393:488	Hence, implant coatings which promote adhesion, proliferation and differentiation of bone-forming cells are desirable.
35008562	1	40	theme	biomaterials	202:213	arg1	biomaterials					202:213	the most prominent biomaterials	183:213	the most prominent biomaterials for bone contact	183:230	Titanium alloy (Ti6Al4V) is one of the most prominent biomaterials for bone contact because of its ability to bear mechanical loading and resist corrosion.
35008562	1	40	theme	biomaterials	202:213	arg1	one					176:178	one	176:178	one	176:178	Titanium alloy (Ti6Al4V) is one of the most prominent biomaterials for bone contact because of its ability to bear mechanical loading and resist corrosion.
35008562	5	41	theme	low	739:741	arg1	heparin					760:766	a low molecular weight heparin	737:766	a low molecular weight heparin LMWH	737:771	In this study, Ti6Al4V substrates produced by additive manufacturing (AM) were coated with whey protein isolate (WPI) fibrils, obtained at pH 2, and heparin or tinzaparin (a low molecular weight heparin LMWH) in order to improve the proliferation and differentiation of bone-forming cells.
35008562	11	42	theme	coated	1532:1537	arg1	substrates					1539:1548	WPI-only coated substrates	1523:1548	WPI-only coated substrates	1523:1548	Indeed, the already known positive effect on osteogenic integration of WPI-only coated substrates has been further enhanced by a simple adsorption procedure.
35008562	5	43	theme	molecular	743:751	arg1	heparin					760:766	a low molecular weight heparin	737:766	a low molecular weight heparin LMWH	737:771	In this study, Ti6Al4V substrates produced by additive manufacturing (AM) were coated with whey protein isolate (WPI) fibrils, obtained at pH 2, and heparin or tinzaparin (a low molecular weight heparin LMWH) in order to improve the proliferation and differentiation of bone-forming cells.
35008562	5	44	theme	isolate	669:675	arg1	fibrils					683:689	whey protein isolate (WPI) fibrils	656:689	whey protein isolate (WPI) fibrils	656:689	In this study, Ti6Al4V substrates produced by additive manufacturing (AM) were coated with whey protein isolate (WPI) fibrils, obtained at pH 2, and heparin or tinzaparin (a low molecular weight heparin LMWH) in order to improve the proliferation and differentiation of bone-forming cells.
35008562	8	45	theme	WPI-heparin-enriched	1049:1068	arg1	coating					1070:1076	This WPI-heparin-enriched coating	1044:1076	This WPI-heparin-enriched coating	1044:1076	This WPI-heparin-enriched coating, especially the LMWH, enhanced the differentiation of hBMSC by increasing tissue non-specific alkaline phosphatase (TNAP) activity.
35008562	8	45	theme	WPI-heparin-enriched	1049:1068	arg1	LMWH					1094:1097	the LMWH	1090:1097	especially the LMWH	1079:1097	This WPI-heparin-enriched coating, especially the LMWH, enhanced the differentiation of hBMSC by increasing tissue non-specific alkaline phosphatase (TNAP) activity.
35008562	11	46	from	effect	1487:1492	arg1	integration					1508:1518	osteogenic integration	1497:1518	osteogenic integration of WPI-only coated substrates	1497:1548	Indeed, the already known positive effect on osteogenic integration of WPI-only coated substrates has been further enhanced by a simple adsorption procedure.
35008562	5	47	theme	weight	753:758	arg1	heparin					760:766	a low molecular weight heparin	737:766	a low molecular weight heparin LMWH	737:771	In this study, Ti6Al4V substrates produced by additive manufacturing (AM) were coated with whey protein isolate (WPI) fibrils, obtained at pH 2, and heparin or tinzaparin (a low molecular weight heparin LMWH) in order to improve the proliferation and differentiation of bone-forming cells.
35008562	6	48	theme	excellent	883:891	arg1	support					893:899	an excellent support	880:899	an excellent support for the growth of human bone marrow stromal cells (hBMSC)	880:957	WPI fibrils proved to be an excellent support for the growth of human bone marrow stromal cells (hBMSC).
35008562	6	48	theme	excellent	883:891	arg1	fibrils					859:865	WPI fibrils	855:865	WPI fibrils	855:865	WPI fibrils proved to be an excellent support for the growth of human bone marrow stromal cells (hBMSC).
35008562	10	49	theme	heat	1435:1438	arg1	treatments					1440:1449	heat treatments	1435:1449	heat treatments	1435:1449	The results confirmed that WPI fibrils are an excellent biomaterial which can be used for biomedical coatings, as they are easily modifiable and resistant to heat treatments.
35008562	2	50	theme	implants	327:334	arg1	success					308:314	The success	304:314	The success of Ti6Al4V implants	304:334	The success of Ti6Al4V implants depends on bone formation on the implant surface.
35008562	5	51	theme	WPI	678:680	arg1	fibrils					683:689	whey protein isolate (WPI) fibrils	656:689	whey protein isolate (WPI) fibrils	656:689	In this study, Ti6Al4V substrates produced by additive manufacturing (AM) were coated with whey protein isolate (WPI) fibrils, obtained at pH 2, and heparin or tinzaparin (a low molecular weight heparin LMWH) in order to improve the proliferation and differentiation of bone-forming cells.
35008562	11	52	theme	positive	1478:1485	arg1	effect					1487:1492	the already known positive effect	1460:1492	the already known positive effect on osteogenic integration of WPI-only coated substrates	1460:1548	Indeed, the already known positive effect on osteogenic integration of WPI-only coated substrates has been further enhanced by a simple adsorption procedure.
35008562	2	53	theme	Ti6Al4V	319:325	arg1	implants					327:334	Ti6Al4V implants	319:334	Ti6Al4V implants	319:334	The success of Ti6Al4V implants depends on bone formation on the implant surface.
35008562	1	54	theme	bone	219:222	arg1	contact					224:230	bone contact	219:230	bone contact	219:230	Titanium alloy (Ti6Al4V) is one of the most prominent biomaterials for bone contact because of its ability to bear mechanical loading and resist corrosion.
35008562	11	55	theme	WPI-only	1523:1530	arg1	substrates					1539:1548	WPI-only coated substrates	1523:1548	WPI-only coated substrates	1523:1548	Indeed, the already known positive effect on osteogenic integration of WPI-only coated substrates has been further enhanced by a simple adsorption procedure.
35008562	1	56	theme	Titanium	148:155	arg1	Ti6Al4V					164:170	Ti6Al4V	164:170	Ti6Al4V	164:170	Titanium alloy (Ti6Al4V) is one of the most prominent biomaterials for bone contact because of its ability to bear mechanical loading and resist corrosion.
35008562	1	56	theme	Titanium	148:155	arg1	alloy					157:161	Titanium alloy	148:161	Titanium alloy (Ti6Al4V)	148:171	Titanium alloy (Ti6Al4V) is one of the most prominent biomaterials for bone contact because of its ability to bear mechanical loading and resist corrosion.
35008562	3	57	theme	bone-forming	471:482	arg1	cells					484:488	bone-forming cells	471:488	bone-forming cells	471:488	Hence, implant coatings which promote adhesion, proliferation and differentiation of bone-forming cells are desirable.
35008562	5	58	theme	bone-forming	835:846	arg1	cells					848:852	bone-forming cells	835:852	bone-forming cells	835:852	In this study, Ti6Al4V substrates produced by additive manufacturing (AM) were coated with whey protein isolate (WPI) fibrils, obtained at pH 2, and heparin or tinzaparin (a low molecular weight heparin LMWH) in order to improve the proliferation and differentiation of bone-forming cells.
35008562	8	59	theme	alkaline	1172:1179	arg1	TNAP					1194:1197	TNAP	1194:1197	TNAP	1194:1197	This WPI-heparin-enriched coating, especially the LMWH, enhanced the differentiation of hBMSC by increasing tissue non-specific alkaline phosphatase (TNAP) activity.
35008562	8	59	theme	alkaline	1172:1179	arg1	phosphatase					1181:1191	tissue non-specific alkaline phosphatase	1152:1191	tissue non-specific alkaline phosphatase (TNAP) activity	1152:1207	This WPI-heparin-enriched coating, especially the LMWH, enhanced the differentiation of hBMSC by increasing tissue non-specific alkaline phosphatase (TNAP) activity.
35008562	11	60	theme	substrates	1539:1548	arg1	integration					1508:1518	osteogenic integration	1497:1518	osteogenic integration of WPI-only coated substrates	1497:1548	Indeed, the already known positive effect on osteogenic integration of WPI-only coated substrates has been further enhanced by a simple adsorption procedure.
35008562	10	61	theme	WPI	1304:1306	arg1	biomaterial					1333:1343	an excellent biomaterial	1320:1343	an excellent biomaterial which can be used for biomedical coatings	1320:1385	The results confirmed that WPI fibrils are an excellent biomaterial which can be used for biomedical coatings, as they are easily modifiable and resistant to heat treatments.
35008562	10	61	theme	WPI	1304:1306	arg1	fibrils					1308:1314	WPI fibrils	1304:1314	WPI fibrils	1304:1314	The results confirmed that WPI fibrils are an excellent biomaterial which can be used for biomedical coatings, as they are easily modifiable and resistant to heat treatments.
35008562	5	62	theme	heparin	760:766	arg1	LMWH					768:771	a low molecular weight heparin LMWH	737:771	a low molecular weight heparin LMWH	737:771	In this study, Ti6Al4V substrates produced by additive manufacturing (AM) were coated with whey protein isolate (WPI) fibrils, obtained at pH 2, and heparin or tinzaparin (a low molecular weight heparin LMWH) in order to improve the proliferation and differentiation of bone-forming cells.
35008562	5	62	theme	heparin	760:766	arg1	heparin					714:720	heparin	714:720	heparin	714:720	In this study, Ti6Al4V substrates produced by additive manufacturing (AM) were coated with whey protein isolate (WPI) fibrils, obtained at pH 2, and heparin or tinzaparin (a low molecular weight heparin LMWH) in order to improve the proliferation and differentiation of bone-forming cells.
33609700	6	0	dep	p < 0.05	1117:1124	arg1	groups					1079:1084	the prebiotic-treated groups	1057:1084	the prebiotic-treated groups relative to the control group	1057:1114	in the prebiotic-treated groups relative to the control group (p < 0.05).
33609700	1	1	theme	gut	352:354	arg1	microflora					356:365	gut microflora	352:365	gut microflora	352:365	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	4	2	theme	basal	900:904	arg1	p < 0.05					912:919	p < 0.05	912:919	p < 0.05	912:919	The carcass protein content was significantly higher in fish nourished by prebiotic at 1.5% of feed for 90 days compared to fish received the basal diet (p < 0.05).
33609700	4	2	theme	basal	900:904	arg1	diet					906:909	the basal diet	896:909	the basal diet (p < 0.05)	896:920	The carcass protein content was significantly higher in fish nourished by prebiotic at 1.5% of feed for 90 days compared to fish received the basal diet (p < 0.05).
33609700	10	3	theme	head	1671:1674	arg1	kidney					1676:1681	the head kidney	1667:1681	the head kidney of fish treated with dietary prebiotic at all intervals	1667:1737	The transcription levels of interleukin 1 beta (IL-1β), interleukin 8 (IL-8) and tumor necrosis factor alpha (TNF-α) were significantly increased in the head kidney of fish treated with dietary prebiotic at all intervals.
33609700	0	4	theme	prebiotic	158:166	arg1	supplementation					168:182	dietary prebiotic supplementation	150:182	dietary prebiotic supplementation	150:182	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.
33609700	1	5	theme	mannan	241:246	arg1	 + β-glucan					269:279	mannan oligosaccharide (MOS) + β-glucan	241:279	mannan oligosaccharide (MOS) + β-glucan (Immunogen®)	241:292	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	1	5	theme	mannan	241:246	arg1	Immunogen®					282:291	Immunogen®	282:291	Immunogen®	282:291	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	2	6	theme	basal	542:546	arg1	diet					548:551	basal diet	542:551	basal diet supplemented with Immunogen®	542:580	Shabout fingerlings (35 ± 1.2 g) were fed with basal diet (control) or basal diet supplemented with Immunogen® at 0.5, 1 and 1.5% of feed for 90 days.
33609700	10	7	theme	dietary	1704:1710	arg1	prebiotic					1712:1720	dietary prebiotic	1704:1720	dietary prebiotic	1704:1720	The transcription levels of interleukin 1 beta (IL-1β), interleukin 8 (IL-8) and tumor necrosis factor alpha (TNF-α) were significantly increased in the head kidney of fish treated with dietary prebiotic at all intervals.
33609700	1	8	theme	proinflammatory	420:434	arg1	cytokines					436:444	some proinflammatory cytokines	415:444	some proinflammatory cytokines	415:444	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	10	9	theme	beta	1560:1563	arg1	levels					1536:1541	The transcription levels	1518:1541	The transcription levels of interleukin 1 beta (IL-1β), interleukin 8 (IL-8) and tumor necrosis factor alpha (TNF-α)	1518:1633	The transcription levels of interleukin 1 beta (IL-1β), interleukin 8 (IL-8) and tumor necrosis factor alpha (TNF-α) were significantly increased in the head kidney of fish treated with dietary prebiotic at all intervals.
33609700	10	10	theme	interleukin	1546:1556	arg1	beta					1560:1563	interleukin 1 beta	1546:1563	interleukin 1 beta (IL-1β)	1546:1571	The transcription levels of interleukin 1 beta (IL-1β), interleukin 8 (IL-8) and tumor necrosis factor alpha (TNF-α) were significantly increased in the head kidney of fish treated with dietary prebiotic at all intervals.
33609700	10	10	theme	interleukin	1546:1556	arg1	IL-1β					1566:1570	IL-1β	1566:1570	IL-1β	1566:1570	The transcription levels of interleukin 1 beta (IL-1β), interleukin 8 (IL-8) and tumor necrosis factor alpha (TNF-α) were significantly increased in the head kidney of fish treated with dietary prebiotic at all intervals.
33609700	11	11	theme	carcass	1878:1884	arg1	protein					1886:1892	carcass protein	1878:1892	carcass protein	1878:1892	The results show that dietary supplementation with Immunogen®, particularly at the level of 1.5%, can positively alter growth parameters, carcass protein, intestinal microflora and immune responses of shabout.
33609700	6	12	theme	control	1102:1108	arg1	group					1110:1114	the control group	1098:1114	the control group	1098:1114	in the prebiotic-treated groups relative to the control group (p < 0.05).
33609700	2	13	theme	basal	518:522	arg1	control					530:536	control	530:536	control	530:536	Shabout fingerlings (35 ± 1.2 g) were fed with basal diet (control) or basal diet supplemented with Immunogen® at 0.5, 1 and 1.5% of feed for 90 days.
33609700	2	13	theme	basal	518:522	arg1	diet					524:527	basal diet	518:527	basal diet (control)	518:537	Shabout fingerlings (35 ± 1.2 g) were fed with basal diet (control) or basal diet supplemented with Immunogen® at 0.5, 1 and 1.5% of feed for 90 days.
33609700	3	14	theme	growth	648:653	arg1	parameters					655:664	growth parameters	648:664	growth parameters	648:664	According to the results, growth parameters were significantly improved in fish fed with prebiotic (1 and 1.5%) for 90 days (p < 0.05).
33609700	1	15	theme	innate	368:373	arg1	responses					382:390	innate immune responses	368:390	innate immune responses	368:390	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	4	16	theme	protein	770:776	arg1	content					778:784	The carcass protein content	758:784	The carcass protein content	758:784	The carcass protein content was significantly higher in fish nourished by prebiotic at 1.5% of feed for 90 days compared to fish received the basal diet (p < 0.05).
33609700	4	16	theme	protein	770:776	arg1	higher					804:809	higher	804:809	higher	804:809	The carcass protein content was significantly higher in fish nourished by prebiotic at 1.5% of feed for 90 days compared to fish received the basal diet (p < 0.05).
33609700	6	17	theme	relative	1086:1093	arg1	groups					1079:1084	the prebiotic-treated groups	1057:1084	the prebiotic-treated groups relative to the control group	1057:1114	in the prebiotic-treated groups relative to the control group (p < 0.05).
33609700	11	18	theme	%	1835:1835	arg1	level					1823:1827	the level	1819:1827	the level of 1.5%	1819:1835	The results show that dietary supplementation with Immunogen®, particularly at the level of 1.5%, can positively alter growth parameters, carcass protein, intestinal microflora and immune responses of shabout.
33609700	6	19	theme	prebiotic-treated	1061:1077	arg1	groups					1079:1084	the prebiotic-treated groups	1057:1084	the prebiotic-treated groups relative to the control group	1057:1114	in the prebiotic-treated groups relative to the control group (p < 0.05).
33609700	1	20	theme	gene	396:399	arg1	expression					401:410	gene expression	396:410	gene expression of some proinflammatory cytokines in shabout (Tor grypus)	396:468	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	10	21	theme	necrosis	1605:1612	arg1	TNF-α					1628:1632	TNF-α	1628:1632	TNF-α	1628:1632	The transcription levels of interleukin 1 beta (IL-1β), interleukin 8 (IL-8) and tumor necrosis factor alpha (TNF-α) were significantly increased in the head kidney of fish treated with dietary prebiotic at all intervals.
33609700	10	21	theme	necrosis	1605:1612	arg1	alpha					1621:1625	tumor necrosis factor alpha	1599:1625	tumor necrosis factor alpha (TNF-α)	1599:1633	The transcription levels of interleukin 1 beta (IL-1β), interleukin 8 (IL-8) and tumor necrosis factor alpha (TNF-α) were significantly increased in the head kidney of fish treated with dietary prebiotic at all intervals.
33609700	2	22	theme	Shabout	471:477	arg1	35 ± 1.2 g					492:501	35 ± 1.2 g	492:501	35 ± 1.2 g	492:501	Shabout fingerlings (35 ± 1.2 g) were fed with basal diet (control) or basal diet supplemented with Immunogen® at 0.5, 1 and 1.5% of feed for 90 days.
33609700	2	22	theme	Shabout	471:477	arg1	fingerlings					479:489	Shabout fingerlings	471:489	Shabout fingerlings (35 ± 1.2 g)	471:502	Shabout fingerlings (35 ± 1.2 g) were fed with basal diet (control) or basal diet supplemented with Immunogen® at 0.5, 1 and 1.5% of feed for 90 days.
33609700	0	23	from	expression	81:90	arg1	shabout					124:130	shabout	124:130	shabout (Tor grypus)	124:143	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.
33609700	0	23	from	expression	81:90	arg1	grypus					137:142	Tor grypus	133:142	Tor grypus	133:142	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.
33609700	7	24	theme	relative	1198:1205	arg1	groups					1191:1196	all prebiotic groups	1177:1196	all prebiotic groups relative to the control group at day 60	1177:1236	Serum total globulin was significantly higher in all prebiotic groups relative to the control group at day 60.
33609700	1	25	from	expression	401:410	arg1	shabout					449:455	shabout	449:455	shabout (Tor grypus)	449:468	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	1	25	from	expression	401:410	arg1	grypus					462:467	Tor grypus	458:467	Tor grypus	458:467	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	1	26	from	composition	339:349	arg1	shabout					449:455	shabout	449:455	shabout (Tor grypus)	449:468	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	1	26	from	composition	339:349	arg1	grypus					462:467	Tor grypus	458:467	Tor grypus	458:467	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	4	27	theme	feed	853:856	arg1	%					848:848	prebiotic at 1.5%	832:848	prebiotic at 1.5% of feed for 90 days compared to fish received the basal diet (p < 0.05)	832:920	The carcass protein content was significantly higher in fish nourished by prebiotic at 1.5% of feed for 90 days compared to fish received the basal diet (p < 0.05).
33609700	4	27	theme	feed	853:856	arg1	feed					853:856	feed	853:856	feed for 90 days compared to fish received the basal diet (p < 0.05)	853:920	The carcass protein content was significantly higher in fish nourished by prebiotic at 1.5% of feed for 90 days compared to fish received the basal diet (p < 0.05).
33609700	1	28	theme	 + β-glucan	269:279	arg1	effect					204:209	the effect	200:209	the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®)	200:292	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	0	29	from	Modulation	0:9	arg1	shabout					124:130	shabout	124:130	shabout (Tor grypus)	124:143	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.
33609700	0	29	from	Modulation	0:9	arg1	grypus					137:142	Tor grypus	133:142	Tor grypus	133:142	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.
33609700	0	30	theme	expression	81:90	arg1	Modulation					0:9	Modulation	0:9	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.	0:183	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.
33609700	5	31	theme	various	936:942	arg1	levels					944:949	various levels	936:949	various levels of Immunogen®	936:963	Feeding with various levels of Immunogen® resulted in the significant promotion of the population of intestinal Lactobacillus spp.
33609700	0	32	from	microflora	38:47	arg1	shabout					124:130	shabout	124:130	shabout (Tor grypus)	124:143	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.
33609700	0	32	from	microflora	38:47	arg1	grypus					137:142	Tor grypus	133:142	Tor grypus	133:142	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.
33609700	0	33	theme	proinflammatory	95:109	arg1	cytokines					111:119	proinflammatory cytokines	95:119	proinflammatory cytokines	95:119	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.
33609700	0	34	theme	gut	34:36	arg1	microflora					38:47	gut microflora	34:47	gut microflora	34:47	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.
33609700	11	35	theme	immune	1921:1926	arg1	responses					1928:1936	immune responses	1921:1936	immune responses	1921:1936	The results show that dietary supplementation with Immunogen®, particularly at the level of 1.5%, can positively alter growth parameters, carcass protein, intestinal microflora and immune responses of shabout.
33609700	2	36	theme	feed	604:607	arg1	feed					604:607	feed	604:607	feed for 90 days	604:619	Shabout fingerlings (35 ± 1.2 g) were fed with basal diet (control) or basal diet supplemented with Immunogen® at 0.5, 1 and 1.5% of feed for 90 days.
33609700	2	36	theme	feed	604:607	arg1	%					599:599	0.5, 1 and 1.5%	585:599	0.5, 1 and 1.5% of feed for 90 days	585:619	Shabout fingerlings (35 ± 1.2 g) were fed with basal diet (control) or basal diet supplemented with Immunogen® at 0.5, 1 and 1.5% of feed for 90 days.
33609700	1	37	theme	MOS	265:267	arg1	 + β-glucan					269:279	mannan oligosaccharide (MOS) + β-glucan	241:279	mannan oligosaccharide (MOS) + β-glucan (Immunogen®)	241:292	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	1	37	theme	MOS	265:267	arg1	Immunogen®					282:291	Immunogen®	282:291	Immunogen®	282:291	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	0	38	theme	growth	14:19	arg1	performance					21:31	growth performance	14:31	growth performance	14:31	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.
33609700	5	39	theme	Lactobacillus	1035:1047	arg1	spp					1049:1051	intestinal Lactobacillus spp	1024:1051	intestinal Lactobacillus spp	1024:1051	Feeding with various levels of Immunogen® resulted in the significant promotion of the population of intestinal Lactobacillus spp.
33609700	7	40	from	day	1231:1233	arg1	group					1222:1226	the control group	1210:1226	the control group at day 60	1210:1236	Serum total globulin was significantly higher in all prebiotic groups relative to the control group at day 60.
33609700	0	41	theme	non-specific	50:61	arg1	immunity					63:70	non-specific immunity	50:70	non-specific immunity	50:70	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.
33609700	9	42	theme	serum	1418:1422	arg1	activities					1437:1446	the highest serum bactericidal activities	1406:1446	the highest serum bactericidal activities	1406:1446	However, the highest serum bactericidal activities were recorded in fish fed with Immunogen® at 1.5% of diet (p < 0.05).
33609700	10	43	theme	alpha	1621:1625	arg1	levels					1536:1541	The transcription levels	1518:1541	The transcription levels of interleukin 1 beta (IL-1β), interleukin 8 (IL-8) and tumor necrosis factor alpha (TNF-α)	1518:1633	The transcription levels of interleukin 1 beta (IL-1β), interleukin 8 (IL-8) and tumor necrosis factor alpha (TNF-α) were significantly increased in the head kidney of fish treated with dietary prebiotic at all intervals.
33609700	0	44	theme	microflora	38:47	arg1	Modulation					0:9	Modulation	0:9	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.	0:183	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.
33609700	7	45	theme	total	1134:1138	arg1	globulin					1140:1147	Serum total globulin	1128:1147	Serum total globulin	1128:1147	Serum total globulin was significantly higher in all prebiotic groups relative to the control group at day 60.
33609700	11	46	theme	dietary	1762:1768	arg1	supplementation					1770:1784	dietary supplementation	1762:1784	dietary supplementation with Immunogen®	1762:1800	The results show that dietary supplementation with Immunogen®, particularly at the level of 1.5%, can positively alter growth parameters, carcass protein, intestinal microflora and immune responses of shabout.
33609700	1	47	theme	dietary	214:220	arg1	supplementation					222:236	dietary supplementation	214:236	dietary supplementation	214:236	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	1	48	from	responses	382:390	arg1	shabout					449:455	shabout	449:455	shabout (Tor grypus)	449:468	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	1	48	from	responses	382:390	arg1	grypus					462:467	Tor grypus	458:467	Tor grypus	458:467	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	8	49	with	feeding	1327:1333	arg1	prebiotic					1348:1356	dietary prebiotic	1340:1356	dietary prebiotic	1340:1356	Serum bactericidal and lysozyme activities were significantly (p < 0.05) elevated after feeding with dietary prebiotic at all intervals (days 30, 60 and 90).
33609700	1	50	theme	oligosaccharide	248:262	arg1	 + β-glucan					269:279	mannan oligosaccharide (MOS) + β-glucan	241:279	mannan oligosaccharide (MOS) + β-glucan (Immunogen®)	241:292	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	1	50	theme	oligosaccharide	248:262	arg1	Immunogen®					282:291	Immunogen®	282:291	Immunogen®	282:291	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	11	51	theme	growth	1859:1864	arg1	parameters					1866:1875	growth parameters	1859:1875	growth parameters	1859:1875	The results show that dietary supplementation with Immunogen®, particularly at the level of 1.5%, can positively alter growth parameters, carcass protein, intestinal microflora and immune responses of shabout.
33609700	1	52	theme	cytokines	436:444	arg1	performance					321:331	growth performance	314:331	growth performance	314:331	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	1	52	theme	cytokines	436:444	arg1	composition					339:349	body composition	334:349	body composition	334:349	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	1	52	theme	cytokines	436:444	arg1	microflora					356:365	gut microflora	352:365	gut microflora	352:365	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	1	52	theme	cytokines	436:444	arg1	responses					382:390	innate immune responses	368:390	innate immune responses	368:390	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	1	52	theme	cytokines	436:444	arg1	expression					401:410	gene expression	396:410	gene expression of some proinflammatory cytokines in shabout (Tor grypus)	396:468	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	11	53	theme	intestinal	1895:1904	arg1	microflora					1906:1915	intestinal microflora	1895:1915	intestinal microflora	1895:1915	The results show that dietary supplementation with Immunogen®, particularly at the level of 1.5%, can positively alter growth parameters, carcass protein, intestinal microflora and immune responses of shabout.
33609700	10	54	theme	fish	1686:1689	arg1	kidney					1676:1681	the head kidney	1667:1681	the head kidney of fish treated with dietary prebiotic at all intervals	1667:1737	The transcription levels of interleukin 1 beta (IL-1β), interleukin 8 (IL-8) and tumor necrosis factor alpha (TNF-α) were significantly increased in the head kidney of fish treated with dietary prebiotic at all intervals.
33609700	10	55	theme	interleukin	1574:1584	arg1	levels					1536:1541	The transcription levels	1518:1541	The transcription levels of interleukin 1 beta (IL-1β), interleukin 8 (IL-8) and tumor necrosis factor alpha (TNF-α)	1518:1633	The transcription levels of interleukin 1 beta (IL-1β), interleukin 8 (IL-8) and tumor necrosis factor alpha (TNF-α) were significantly increased in the head kidney of fish treated with dietary prebiotic at all intervals.
33609700	9	56	theme	diet	1501:1504	arg1	%					1496:1496	1.5%	1493:1496	1.5% of diet (p < 0.05)	1493:1515	However, the highest serum bactericidal activities were recorded in fish fed with Immunogen® at 1.5% of diet (p < 0.05).
33609700	9	56	theme	diet	1501:1504	arg1	p < 0.05					1507:1514	p < 0.05	1507:1514	p < 0.05	1507:1514	However, the highest serum bactericidal activities were recorded in fish fed with Immunogen® at 1.5% of diet (p < 0.05).
33609700	9	56	theme	diet	1501:1504	arg1	diet					1501:1504	diet	1501:1504	diet (p < 0.05)	1501:1515	However, the highest serum bactericidal activities were recorded in fish fed with Immunogen® at 1.5% of diet (p < 0.05).
33609700	1	57	theme	Tor	458:460	arg1	shabout					449:455	shabout	449:455	shabout (Tor grypus)	449:468	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	1	57	theme	Tor	458:460	arg1	grypus					462:467	Tor grypus	458:467	Tor grypus	458:467	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	0	58	theme	Tor	133:135	arg1	shabout					124:130	shabout	124:130	shabout (Tor grypus)	124:143	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.
33609700	0	58	theme	Tor	133:135	arg1	grypus					137:142	Tor grypus	133:142	Tor grypus	133:142	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.
33609700	0	59	from	immunity	63:70	arg1	shabout					124:130	shabout	124:130	shabout (Tor grypus)	124:143	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.
33609700	0	59	from	immunity	63:70	arg1	grypus					137:142	Tor grypus	133:142	Tor grypus	133:142	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.
33609700	1	60	theme	immune	375:380	arg1	responses					382:390	innate immune responses	368:390	innate immune responses	368:390	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	8	61	dep	significantly	1287:1299	arg1	p < 0.05					1302:1309	p < 0.05	1302:1309	p < 0.05	1302:1309	Serum bactericidal and lysozyme activities were significantly (p < 0.05) elevated after feeding with dietary prebiotic at all intervals (days 30, 60 and 90).
33609700	4	62	theme	carcass	762:768	arg1	content					778:784	The carcass protein content	758:784	The carcass protein content	758:784	The carcass protein content was significantly higher in fish nourished by prebiotic at 1.5% of feed for 90 days compared to fish received the basal diet (p < 0.05).
33609700	4	62	theme	carcass	762:768	arg1	higher					804:809	higher	804:809	higher	804:809	The carcass protein content was significantly higher in fish nourished by prebiotic at 1.5% of feed for 90 days compared to fish received the basal diet (p < 0.05).
33609700	0	63	theme	dietary	150:156	arg1	supplementation					168:182	dietary prebiotic supplementation	150:182	dietary prebiotic supplementation	150:182	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.
33609700	10	64	theme	factor	1614:1619	arg1	TNF-α					1628:1632	TNF-α	1628:1632	TNF-α	1628:1632	The transcription levels of interleukin 1 beta (IL-1β), interleukin 8 (IL-8) and tumor necrosis factor alpha (TNF-α) were significantly increased in the head kidney of fish treated with dietary prebiotic at all intervals.
33609700	10	64	theme	factor	1614:1619	arg1	alpha					1621:1625	tumor necrosis factor alpha	1599:1625	tumor necrosis factor alpha (TNF-α)	1599:1633	The transcription levels of interleukin 1 beta (IL-1β), interleukin 8 (IL-8) and tumor necrosis factor alpha (TNF-α) were significantly increased in the head kidney of fish treated with dietary prebiotic at all intervals.
33609700	8	65	theme	dietary	1340:1346	arg1	prebiotic					1348:1356	dietary prebiotic	1340:1356	dietary prebiotic	1340:1356	Serum bactericidal and lysozyme activities were significantly (p < 0.05) elevated after feeding with dietary prebiotic at all intervals (days 30, 60 and 90).
33609700	7	66	theme	control	1214:1220	arg1	group					1222:1226	the control group	1210:1226	the control group at day 60	1210:1236	Serum total globulin was significantly higher in all prebiotic groups relative to the control group at day 60.
33609700	0	67	theme	immunity	63:70	arg1	Modulation					0:9	Modulation	0:9	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.	0:183	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.
33609700	10	68	theme	tumor	1599:1603	arg1	TNF-α					1628:1632	TNF-α	1628:1632	TNF-α	1628:1632	The transcription levels of interleukin 1 beta (IL-1β), interleukin 8 (IL-8) and tumor necrosis factor alpha (TNF-α) were significantly increased in the head kidney of fish treated with dietary prebiotic at all intervals.
33609700	10	68	theme	tumor	1599:1603	arg1	alpha					1621:1625	tumor necrosis factor alpha	1599:1625	tumor necrosis factor alpha (TNF-α)	1599:1633	The transcription levels of interleukin 1 beta (IL-1β), interleukin 8 (IL-8) and tumor necrosis factor alpha (TNF-α) were significantly increased in the head kidney of fish treated with dietary prebiotic at all intervals.
33609700	0	69	from	performance	21:31	arg1	shabout					124:130	shabout	124:130	shabout (Tor grypus)	124:143	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.
33609700	0	69	from	performance	21:31	arg1	grypus					137:142	Tor grypus	133:142	Tor grypus	133:142	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.
33609700	0	70	theme	gene	76:79	arg1	expression					81:90	gene expression	76:90	gene expression of proinflammatory cytokines in shabout (Tor grypus)	76:143	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.
33609700	1	71	theme	growth	314:319	arg1	performance					321:331	growth performance	314:331	growth performance	314:331	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	1	72	theme	body	334:337	arg1	composition					339:349	body composition	334:349	body composition	334:349	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	5	73	theme	significant	981:991	arg1	promotion					993:1001	the significant promotion	977:1001	the significant promotion of the population of intestinal Lactobacillus spp	977:1051	Feeding with various levels of Immunogen® resulted in the significant promotion of the population of intestinal Lactobacillus spp.
33609700	0	74	theme	cytokines	111:119	arg1	performance					21:31	growth performance	14:31	growth performance	14:31	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.
33609700	0	74	theme	cytokines	111:119	arg1	microflora					38:47	gut microflora	34:47	gut microflora	34:47	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.
33609700	0	74	theme	cytokines	111:119	arg1	immunity					63:70	non-specific immunity	50:70	non-specific immunity	50:70	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.
33609700	0	74	theme	cytokines	111:119	arg1	expression					81:90	gene expression	76:90	gene expression of proinflammatory cytokines in shabout (Tor grypus)	76:143	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.
33609700	7	75	theme	prebiotic	1181:1189	arg1	groups					1191:1196	all prebiotic groups	1177:1196	all prebiotic groups relative to the control group at day 60	1177:1236	Serum total globulin was significantly higher in all prebiotic groups relative to the control group at day 60.
33609700	1	76	from	microflora	356:365	arg1	shabout					449:455	shabout	449:455	shabout (Tor grypus)	449:468	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	1	76	from	microflora	356:365	arg1	grypus					462:467	Tor grypus	458:467	Tor grypus	458:467	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	5	77	theme	Immunogen®	954:963	arg1	levels					944:949	various levels	936:949	various levels of Immunogen®	936:963	Feeding with various levels of Immunogen® resulted in the significant promotion of the population of intestinal Lactobacillus spp.
33609700	5	78	with	Feeding	923:929	arg1	levels					944:949	various levels	936:949	various levels of Immunogen®	936:963	Feeding with various levels of Immunogen® resulted in the significant promotion of the population of intestinal Lactobacillus spp.
33609700	0	79	theme	performance	21:31	arg1	Modulation					0:9	Modulation	0:9	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.	0:183	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.
33609700	5	80	theme	intestinal	1024:1033	arg1	spp					1049:1051	intestinal Lactobacillus spp	1024:1051	intestinal Lactobacillus spp	1024:1051	Feeding with various levels of Immunogen® resulted in the significant promotion of the population of intestinal Lactobacillus spp.
33609700	1	81	from	performance	321:331	arg1	shabout					449:455	shabout	449:455	shabout (Tor grypus)	449:468	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	1	81	from	performance	321:331	arg1	grypus					462:467	Tor grypus	458:467	Tor grypus	458:467	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
33609700	5	82	theme	spp	1049:1051	arg1	population					1010:1019	the population	1006:1019	the population of intestinal Lactobacillus spp	1006:1051	Feeding with various levels of Immunogen® resulted in the significant promotion of the population of intestinal Lactobacillus spp.
33609700	9	83	theme	highest	1410:1416	arg1	activities					1437:1446	the highest serum bactericidal activities	1406:1446	the highest serum bactericidal activities	1406:1446	However, the highest serum bactericidal activities were recorded in fish fed with Immunogen® at 1.5% of diet (p < 0.05).
33609700	11	84	with	supplementation	1770:1784	arg1	Immunogen®					1791:1800	Immunogen®	1791:1800	Immunogen®	1791:1800	The results show that dietary supplementation with Immunogen®, particularly at the level of 1.5%, can positively alter growth parameters, carcass protein, intestinal microflora and immune responses of shabout.
33609700	9	85	theme	bactericidal	1424:1435	arg1	activities					1437:1446	the highest serum bactericidal activities	1406:1446	the highest serum bactericidal activities	1406:1446	However, the highest serum bactericidal activities were recorded in fish fed with Immunogen® at 1.5% of diet (p < 0.05).
33609700	7	86	theme	Serum	1128:1132	arg1	globulin					1140:1147	Serum total globulin	1128:1147	Serum total globulin	1128:1147	Serum total globulin was significantly higher in all prebiotic groups relative to the control group at day 60.
33609700	11	87	theme	shabout	1941:1947	arg1	parameters					1866:1875	growth parameters	1859:1875	growth parameters	1859:1875	The results show that dietary supplementation with Immunogen®, particularly at the level of 1.5%, can positively alter growth parameters, carcass protein, intestinal microflora and immune responses of shabout.
33609700	11	87	theme	shabout	1941:1947	arg1	protein					1886:1892	carcass protein	1878:1892	carcass protein	1878:1892	The results show that dietary supplementation with Immunogen®, particularly at the level of 1.5%, can positively alter growth parameters, carcass protein, intestinal microflora and immune responses of shabout.
33609700	11	87	theme	shabout	1941:1947	arg1	microflora					1906:1915	intestinal microflora	1895:1915	intestinal microflora	1895:1915	The results show that dietary supplementation with Immunogen®, particularly at the level of 1.5%, can positively alter growth parameters, carcass protein, intestinal microflora and immune responses of shabout.
33609700	11	87	theme	shabout	1941:1947	arg1	responses					1928:1936	immune responses	1921:1936	immune responses	1921:1936	The results show that dietary supplementation with Immunogen®, particularly at the level of 1.5%, can positively alter growth parameters, carcass protein, intestinal microflora and immune responses of shabout.
33609700	5	88	theme	population	1010:1019	arg1	promotion					993:1001	the significant promotion	977:1001	the significant promotion of the population of intestinal Lactobacillus spp	977:1051	Feeding with various levels of Immunogen® resulted in the significant promotion of the population of intestinal Lactobacillus spp.
33609700	8	89	theme	lysozyme	1262:1269	arg1	activities					1271:1280	Serum bactericidal and lysozyme activities	1239:1280	activities	1271:1280	Serum bactericidal and lysozyme activities were significantly (p < 0.05) elevated after feeding with dietary prebiotic at all intervals (days 30, 60 and 90).
33609700	10	90	theme	transcription	1522:1534	arg1	levels					1536:1541	The transcription levels	1518:1541	The transcription levels of interleukin 1 beta (IL-1β), interleukin 8 (IL-8) and tumor necrosis factor alpha (TNF-α)	1518:1633	The transcription levels of interleukin 1 beta (IL-1β), interleukin 8 (IL-8) and tumor necrosis factor alpha (TNF-α) were significantly increased in the head kidney of fish treated with dietary prebiotic at all intervals.
33609700	0	91	from	shabout	124:130	arg1	Modulation					0:9	Modulation	0:9	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.	0:183	Modulation of growth performance, gut microflora, non-specific immunity and gene expression of proinflammatory cytokines in shabout (Tor grypus) upon dietary prebiotic supplementation.
33609700	1	92	theme	supplementation	222:236	arg1	effect					204:209	the effect	200:209	the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®)	200:292	In this study, the effect of dietary supplementation of mannan oligosaccharide (MOS) + β-glucan (Immunogen®) was investigated on growth performance, body composition, gut microflora, innate immune responses and gene expression of some proinflammatory cytokines in shabout (Tor grypus).
32387236	10	0	theme	DSS-induced	1453:1463	arg1	colitis					1465:1471	DSS-induced colitis	1453:1471	DSS-induced colitis	1453:1471	RESULTS Daily oral administration of HHS markedly alleviated DSS-induced colitis, as evidenced by decreased colitis disease activity index (DAI) score, reduced colonic inflammation and normalization of colonic vascular hyperpermeability.
32387236	9	1	theme	16S	1317:1319	arg1	rRNA					1321:1324	16S rRNA	1317:1324	the 16S rRNA gene	1313:1329	Meanwhile, The effects of the HHS therapy on the colonic microbiota were evaluated by analyzing the V3 and V4 regions of the 16S rRNA gene by Illumina sequencing and multivariate statistical methods.
32387236	12	2	theme	increased	1974:1982	arg1	ratio					1984:1988	colitis-associated high increased ratio	1950:1988	colitis-associated high increased ratio of Bacteroidetes to Firmicutes	1950:2019	In addition, HHS therapy reduced colitis-associated high increased ratio of Bacteroidetes to Firmicutes to a normal level.
32387236	10	3	theme	decreased	1490:1498	arg1	score					1537:1541	decreased colitis disease activity index (DAI) score	1490:1541	decreased colitis disease activity index (DAI) score	1490:1541	RESULTS Daily oral administration of HHS markedly alleviated DSS-induced colitis, as evidenced by decreased colitis disease activity index (DAI) score, reduced colonic inflammation and normalization of colonic vascular hyperpermeability.
32387236	3	4	theme	mechanism	493:501	arg1	evidence					454:461	scientific evidence	443:461	scientific evidence of the efficacy and the exact mechanism of HHS against colitis	443:524	However, scientific evidence of the efficacy and the exact mechanism of HHS against colitis has not yet been reported.
32387236	9	5	theme	gene	1326:1329	arg1	regions					1302:1308	the V3 and V4 regions	1288:1308	the V3 and V4 regions of the 16S rRNA gene	1288:1329	Meanwhile, The effects of the HHS therapy on the colonic microbiota were evaluated by analyzing the V3 and V4 regions of the 16S rRNA gene by Illumina sequencing and multivariate statistical methods.
32387236	4	6	theme	composition	748:758	arg1	alteration					715:724	the alteration	711:724	the alteration of colonic microbiota composition and structure	711:772	AIM OF THE STUDY The aim of this study is to investigate the potential effects of HHS in the alleviation of dextran sulphate sodium (DSS)-induced colitis and the alteration of colonic microbiota composition and structure.
32387236	4	6	theme	composition	748:758	arg1	alleviation					646:656	the alleviation	642:656	the alleviation of dextran sulphate sodium (DSS)-induced colitis	642:705	AIM OF THE STUDY The aim of this study is to investigate the potential effects of HHS in the alleviation of dextran sulphate sodium (DSS)-induced colitis and the alteration of colonic microbiota composition and structure.
32387236	1	7	theme	herbal	173:178	arg1	formula					180:186	a traditional Chinese herbal formula	151:186	a traditional Chinese herbal formula which is firstly documented in the ancient Chinese classic medical work "Pu Ji Ben Shi Fang" in 1132 AD	151:290	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS) is a traditional Chinese herbal formula which is firstly documented in the ancient Chinese classic medical work "Pu Ji Ben Shi Fang" in 1132 AD.
32387236	1	7	theme	herbal	173:178	arg1	HHS					143:145	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS)	98:146	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS)	98:146	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS) is a traditional Chinese herbal formula which is firstly documented in the ancient Chinese classic medical work "Pu Ji Ben Shi Fang" in 1132 AD.
32387236	4	8	theme	structure	764:772	arg1	alteration					715:724	the alteration	711:724	the alteration of colonic microbiota composition and structure	711:772	AIM OF THE STUDY The aim of this study is to investigate the potential effects of HHS in the alleviation of dextran sulphate sodium (DSS)-induced colitis and the alteration of colonic microbiota composition and structure.
32387236	4	8	theme	structure	764:772	arg1	alleviation					646:656	the alleviation	642:656	the alleviation of dextran sulphate sodium (DSS)-induced colitis	642:705	AIM OF THE STUDY The aim of this study is to investigate the potential effects of HHS in the alleviation of dextran sulphate sodium (DSS)-induced colitis and the alteration of colonic microbiota composition and structure.
32387236	8	9	theme	blue	1172:1175	arg1	extravasation					1177:1189	Evans blue extravasation	1166:1189	Evans blue extravasation	1166:1189	The colonic vascular permeability was evaluated by Evans blue extravasation.
32387236	10	10	theme	index	1525:1529	arg1	score					1537:1541	decreased colitis disease activity index (DAI) score	1490:1541	decreased colitis disease activity index (DAI) score	1490:1541	RESULTS Daily oral administration of HHS markedly alleviated DSS-induced colitis, as evidenced by decreased colitis disease activity index (DAI) score, reduced colonic inflammation and normalization of colonic vascular hyperpermeability.
32387236	11	11	theme	colitis-associated	1739:1756	arg1	alteration					1758:1767	the colitis-associated alteration	1735:1767	the colitis-associated alteration of colonic microbial community at operational taxonomic unit level	1735:1834	Moreover, the 16S rRNA gene sequencing analysis demonstrated that HHS treatment during colitis prevented the colitis-associated alteration of colonic microbial community at operational taxonomic unit level, together with the DSS-induced colonic microbiota dysbiosis at taxonomic levels.
32387236	5	12	theme	METHODS	789:795	arg1	solution					801:808	MATERIALS AND METHODS HHS solution	775:808	MATERIALS AND METHODS HHS solution	775:808	MATERIALS AND METHODS HHS solution was orally administrated to 5% DSS-challenged rats once a day for 8 days.
32387236	12	13	theme	colitis-associated	1950:1967	arg1	ratio					1984:1988	colitis-associated high increased ratio	1950:1988	colitis-associated high increased ratio of Bacteroidetes to Firmicutes	1950:2019	In addition, HHS therapy reduced colitis-associated high increased ratio of Bacteroidetes to Firmicutes to a normal level.
32387236	4	14	theme	STUDY	564:568	arg1	AIM					553:555	AIM	553:555	AIM OF THE STUDY The aim of this study is to investigate the potential effects of HHS in the alleviation of dextran sulphate sodium (DSS)-induced colitis and the alteration of colonic microbiota composition and structure.	553:773	AIM OF THE STUDY The aim of this study is to investigate the potential effects of HHS in the alleviation of dextran sulphate sodium (DSS)-induced colitis and the alteration of colonic microbiota composition and structure.
32387236	10	15	theme	disease	1508:1514	arg1	index					1525:1529	colitis disease activity index	1500:1529	decreased colitis disease activity index (DAI) score	1490:1541	RESULTS Daily oral administration of HHS markedly alleviated DSS-induced colitis, as evidenced by decreased colitis disease activity index (DAI) score, reduced colonic inflammation and normalization of colonic vascular hyperpermeability.
32387236	10	15	theme	disease	1508:1514	arg1	DAI					1532:1534	DAI	1532:1534	DAI	1532:1534	RESULTS Daily oral administration of HHS markedly alleviated DSS-induced colitis, as evidenced by decreased colitis disease activity index (DAI) score, reduced colonic inflammation and normalization of colonic vascular hyperpermeability.
32387236	13	16	theme	gut	2105:2107	arg1	dysbiosis					2119:2127	gut microbial dysbiosis	2105:2127	gut microbial dysbiosis	2105:2127	CONCLUSION HHS could attenuate ulcerative colitis and ameliorate gut microbial dysbiosis.
32387236	3	17	theme	HHS	506:508	arg1	mechanism					493:501	the exact mechanism	483:501	the exact mechanism	483:501	However, scientific evidence of the efficacy and the exact mechanism of HHS against colitis has not yet been reported.
32387236	3	17	theme	HHS	506:508	arg1	efficacy					470:477	efficacy	470:477	efficacy	470:477	However, scientific evidence of the efficacy and the exact mechanism of HHS against colitis has not yet been reported.
32387236	9	18	theme	statistical	1371:1381	arg1	methods					1383:1389	multivariate statistical methods	1358:1389	multivariate statistical methods	1358:1389	Meanwhile, The effects of the HHS therapy on the colonic microbiota were evaluated by analyzing the V3 and V4 regions of the 16S rRNA gene by Illumina sequencing and multivariate statistical methods.
32387236	8	19	theme	vascular	1127:1134	arg1	permeability					1136:1147	The colonic vascular permeability	1115:1147	The colonic vascular permeability	1115:1147	The colonic vascular permeability was evaluated by Evans blue extravasation.
32387236	11	20	theme	DSS-induced	1855:1865	arg1	dysbiosis					1886:1894	the DSS-induced colonic microbiota dysbiosis	1851:1894	the DSS-induced colonic microbiota dysbiosis at taxonomic levels	1851:1914	Moreover, the 16S rRNA gene sequencing analysis demonstrated that HHS treatment during colitis prevented the colitis-associated alteration of colonic microbial community at operational taxonomic unit level, together with the DSS-induced colonic microbiota dysbiosis at taxonomic levels.
32387236	11	21	theme	microbial	1780:1788	arg1	community					1790:1798	colonic microbial community	1772:1798	colonic microbial community	1772:1798	Moreover, the 16S rRNA gene sequencing analysis demonstrated that HHS treatment during colitis prevented the colitis-associated alteration of colonic microbial community at operational taxonomic unit level, together with the DSS-induced colonic microbiota dysbiosis at taxonomic levels.
32387236	13	22	theme	ulcerative	2071:2080	arg1	colitis					2082:2088	ulcerative colitis	2071:2088	ulcerative colitis	2071:2088	CONCLUSION HHS could attenuate ulcerative colitis and ameliorate gut microbial dysbiosis.
32387236	1	23	theme	san	138:140	arg1	formula					180:186	a traditional Chinese herbal formula	151:186	a traditional Chinese herbal formula which is firstly documented in the ancient Chinese classic medical work "Pu Ji Ben Shi Fang" in 1132 AD	151:290	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS) is a traditional Chinese herbal formula which is firstly documented in the ancient Chinese classic medical work "Pu Ji Ben Shi Fang" in 1132 AD.
32387236	1	23	theme	san	138:140	arg1	HHS					143:145	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS)	98:146	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS)	98:146	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS) is a traditional Chinese herbal formula which is firstly documented in the ancient Chinese classic medical work "Pu Ji Ben Shi Fang" in 1132 AD.
32387236	11	24	from	levels	1909:1914	arg1	dysbiosis					1886:1894	the DSS-induced colonic microbiota dysbiosis	1851:1894	the DSS-induced colonic microbiota dysbiosis at taxonomic levels	1851:1914	Moreover, the 16S rRNA gene sequencing analysis demonstrated that HHS treatment during colitis prevented the colitis-associated alteration of colonic microbial community at operational taxonomic unit level, together with the DSS-induced colonic microbiota dysbiosis at taxonomic levels.
32387236	5	25	theme	%	839:839	arg1	rats					856:859	5% DSS-challenged rats	838:859	5% DSS-challenged rats	838:859	MATERIALS AND METHODS HHS solution was orally administrated to 5% DSS-challenged rats once a day for 8 days.
32387236	2	26	theme	acute	378:382	arg1	colitis					384:390	acute colitis	378:390	acute colitis	378:390	It has been widely used in the treatment of lower gastrointestinal disorders such as acute colitis and hematochezia for more than 800 years.
32387236	12	27	theme	normal	2026:2031	arg1	level					2033:2037	a normal level	2024:2037	a normal level	2024:2037	In addition, HHS therapy reduced colitis-associated high increased ratio of Bacteroidetes to Firmicutes to a normal level.
32387236	11	28	theme	sequencing	1658:1667	arg1	analysis					1669:1676	the 16S rRNA gene sequencing analysis	1640:1676	the 16S rRNA gene sequencing analysis	1640:1676	Moreover, the 16S rRNA gene sequencing analysis demonstrated that HHS treatment during colitis prevented the colitis-associated alteration of colonic microbial community at operational taxonomic unit level, together with the DSS-induced colonic microbiota dysbiosis at taxonomic levels.
32387236	13	29	theme	CONCLUSION	2040:2049	arg1	HHS					2051:2053	CONCLUSION HHS	2040:2053	CONCLUSION HHS	2040:2053	CONCLUSION HHS could attenuate ulcerative colitis and ameliorate gut microbial dysbiosis.
32387236	4	30	theme	potential	614:622	arg1	effects					624:630	the potential effects	610:630	the potential effects of HHS in the alleviation of dextran sulphate sodium (DSS)-induced colitis and the alteration of colonic microbiota composition and structure	610:772	AIM OF THE STUDY The aim of this study is to investigate the potential effects of HHS in the alleviation of dextran sulphate sodium (DSS)-induced colitis and the alteration of colonic microbiota composition and structure.
32387236	0	31	theme	colonic	78:84	arg1	microbiota					86:95	colonic microbiota	78:95	colonic microbiota	78:95	Huai hua san alleviates dextran sulphate sodium-induced colitis and modulates colonic microbiota.
32387236	1	32	theme	traditional	153:163	arg1	formula					180:186	a traditional Chinese herbal formula	151:186	a traditional Chinese herbal formula which is firstly documented in the ancient Chinese classic medical work "Pu Ji Ben Shi Fang" in 1132 AD	151:290	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS) is a traditional Chinese herbal formula which is firstly documented in the ancient Chinese classic medical work "Pu Ji Ben Shi Fang" in 1132 AD.
32387236	1	32	theme	traditional	153:163	arg1	HHS					143:145	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS)	98:146	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS)	98:146	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS) is a traditional Chinese herbal formula which is firstly documented in the ancient Chinese classic medical work "Pu Ji Ben Shi Fang" in 1132 AD.
32387236	6	33	theme	clinical	892:899	arg1	symptoms					901:908	Colitis clinical symptoms	884:908	Colitis clinical symptoms of colitis	884:919	Colitis clinical symptoms of colitis were collected, together with colonic mucosal damage assessed at histomorphometric and ultrastructural levels.
32387236	2	34	used	used	312:315	arg2	It					293:294	It	293:294	It	293:294	It has been widely used in the treatment of lower gastrointestinal disorders such as acute colitis and hematochezia for more than 800 years.
32387236	1	35	theme	Huai	129:132	arg1	formula					180:186	a traditional Chinese herbal formula	151:186	a traditional Chinese herbal formula which is firstly documented in the ancient Chinese classic medical work "Pu Ji Ben Shi Fang" in 1132 AD	151:290	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS) is a traditional Chinese herbal formula which is firstly documented in the ancient Chinese classic medical work "Pu Ji Ben Shi Fang" in 1132 AD.
32387236	1	35	theme	Huai	129:132	arg1	HHS					143:145	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS)	98:146	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS)	98:146	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS) is a traditional Chinese herbal formula which is firstly documented in the ancient Chinese classic medical work "Pu Ji Ben Shi Fang" in 1132 AD.
32387236	11	36	theme	HHS	1696:1698	arg1	treatment					1700:1708	HHS treatment	1696:1708	HHS treatment during colitis	1696:1723	Moreover, the 16S rRNA gene sequencing analysis demonstrated that HHS treatment during colitis prevented the colitis-associated alteration of colonic microbial community at operational taxonomic unit level, together with the DSS-induced colonic microbiota dysbiosis at taxonomic levels.
32387236	10	37	dep	RESULTS	1392:1398	arg1	alleviated					1442:1451	alleviated	1442:1451	alleviated	1442:1451	RESULTS Daily oral administration of HHS markedly alleviated DSS-induced colitis, as evidenced by decreased colitis disease activity index (DAI) score, reduced colonic inflammation and normalization of colonic vascular hyperpermeability.
32387236	9	38	theme	HHS	1222:1224	arg1	therapy					1226:1232	the HHS therapy	1218:1232	the HHS therapy	1218:1232	Meanwhile, The effects of the HHS therapy on the colonic microbiota were evaluated by analyzing the V3 and V4 regions of the 16S rRNA gene by Illumina sequencing and multivariate statistical methods.
32387236	2	39	theme	gastrointestinal	343:358	arg1	hematochezia					396:407	hematochezia	396:407	hematochezia	396:407	It has been widely used in the treatment of lower gastrointestinal disorders such as acute colitis and hematochezia for more than 800 years.
32387236	2	39	theme	gastrointestinal	343:358	arg1	colitis					384:390	acute colitis	378:390	acute colitis	378:390	It has been widely used in the treatment of lower gastrointestinal disorders such as acute colitis and hematochezia for more than 800 years.
32387236	2	39	theme	gastrointestinal	343:358	arg1	disorders					360:368	lower gastrointestinal disorders	337:368	lower gastrointestinal disorders such as acute colitis and hematochezia	337:407	It has been widely used in the treatment of lower gastrointestinal disorders such as acute colitis and hematochezia for more than 800 years.
32387236	0	40	theme	sulphate	32:39	arg1	colitis					56:62	dextran sulphate sodium-induced colitis	24:62	dextran sulphate sodium-induced colitis	24:62	Huai hua san alleviates dextran sulphate sodium-induced colitis and modulates colonic microbiota.
32387236	7	41	dep	mediators	1067:1075	arg1	mediators					1067:1075	inflammatory mediators TNF-α and CRP	1054:1089	inflammatory mediators TNF-α and CRP	1054:1089	The protein levels of inflammatory mediators TNF-α and CRP were detected by ELISA.
32387236	7	41	dep	mediators	1067:1075	arg1	CRP					1087:1089	CRP	1087:1089	CRP	1087:1089	The protein levels of inflammatory mediators TNF-α and CRP were detected by ELISA.
32387236	7	41	dep	mediators	1067:1075	arg1	TNF-α					1077:1081	TNF-α	1077:1081	TNF-α	1077:1081	The protein levels of inflammatory mediators TNF-α and CRP were detected by ELISA.
32387236	10	42	theme	colonic	1552:1558	arg1	inflammation					1560:1571	reduced colonic inflammation	1544:1571	reduced colonic inflammation	1544:1571	RESULTS Daily oral administration of HHS markedly alleviated DSS-induced colitis, as evidenced by decreased colitis disease activity index (DAI) score, reduced colonic inflammation and normalization of colonic vascular hyperpermeability.
32387236	0	43	theme	Huai	0:3	arg1	san					9:11	Huai hua san	0:11	Huai hua san	0:11	Huai hua san alleviates dextran sulphate sodium-induced colitis and modulates colonic microbiota.
32387236	9	44	theme	colonic	1241:1247	arg1	microbiota					1249:1258	the colonic microbiota	1237:1258	the colonic microbiota	1237:1258	Meanwhile, The effects of the HHS therapy on the colonic microbiota were evaluated by analyzing the V3 and V4 regions of the 16S rRNA gene by Illumina sequencing and multivariate statistical methods.
32387236	1	45	theme	ETHNOPHARMACOLOGICAL	98:117	arg1	formula					180:186	a traditional Chinese herbal formula	151:186	a traditional Chinese herbal formula which is firstly documented in the ancient Chinese classic medical work "Pu Ji Ben Shi Fang" in 1132 AD	151:290	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS) is a traditional Chinese herbal formula which is firstly documented in the ancient Chinese classic medical work "Pu Ji Ben Shi Fang" in 1132 AD.
32387236	1	45	theme	ETHNOPHARMACOLOGICAL	98:117	arg1	HHS					143:145	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS)	98:146	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS)	98:146	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS) is a traditional Chinese herbal formula which is firstly documented in the ancient Chinese classic medical work "Pu Ji Ben Shi Fang" in 1132 AD.
32387236	6	46	theme	mucosal	959:965	arg1	damage					967:972	colonic mucosal damage	951:972	colonic mucosal damage assessed at histomorphometric and ultrastructural levels	951:1029	Colitis clinical symptoms of colitis were collected, together with colonic mucosal damage assessed at histomorphometric and ultrastructural levels.
32387236	10	47	theme	vascular	1602:1609	arg1	hyperpermeability					1611:1627	colonic vascular hyperpermeability	1594:1627	colonic vascular hyperpermeability	1594:1627	RESULTS Daily oral administration of HHS markedly alleviated DSS-induced colitis, as evidenced by decreased colitis disease activity index (DAI) score, reduced colonic inflammation and normalization of colonic vascular hyperpermeability.
32387236	3	48	dep	efficacy	470:477	arg1	the					466:468	the	466:468	the	466:468	However, scientific evidence of the efficacy and the exact mechanism of HHS against colitis has not yet been reported.
32387236	10	49	theme	Daily	1400:1404	arg1	administration					1411:1424	Daily oral administration	1400:1424	Daily oral administration of HHS	1400:1431	RESULTS Daily oral administration of HHS markedly alleviated DSS-induced colitis, as evidenced by decreased colitis disease activity index (DAI) score, reduced colonic inflammation and normalization of colonic vascular hyperpermeability.
32387236	0	50	theme	dextran	24:30	arg1	colitis					56:62	dextran sulphate sodium-induced colitis	24:62	dextran sulphate sodium-induced colitis	24:62	Huai hua san alleviates dextran sulphate sodium-induced colitis and modulates colonic microbiota.
32387236	9	51	theme	Illumina	1334:1341	arg1	sequencing					1343:1352	Illumina sequencing	1334:1352	Illumina sequencing	1334:1352	Meanwhile, The effects of the HHS therapy on the colonic microbiota were evaluated by analyzing the V3 and V4 regions of the 16S rRNA gene by Illumina sequencing and multivariate statistical methods.
32387236	4	52	theme	colonic	729:735	arg1	composition					748:758	colonic microbiota composition	729:758	colonic microbiota composition	729:758	AIM OF THE STUDY The aim of this study is to investigate the potential effects of HHS in the alleviation of dextran sulphate sodium (DSS)-induced colitis and the alteration of colonic microbiota composition and structure.
32387236	11	53	theme	taxonomic	1815:1823	arg1	unit					1825:1828	operational taxonomic unit	1803:1828	operational taxonomic unit level	1803:1834	Moreover, the 16S rRNA gene sequencing analysis demonstrated that HHS treatment during colitis prevented the colitis-associated alteration of colonic microbial community at operational taxonomic unit level, together with the DSS-induced colonic microbiota dysbiosis at taxonomic levels.
32387236	7	54	theme	inflammatory	1054:1065	arg1	mediators					1067:1075	inflammatory mediators TNF-α and CRP	1054:1089	inflammatory mediators TNF-α and CRP	1054:1089	The protein levels of inflammatory mediators TNF-α and CRP were detected by ELISA.
32387236	7	54	theme	inflammatory	1054:1065	arg1	CRP					1087:1089	CRP	1087:1089	CRP	1087:1089	The protein levels of inflammatory mediators TNF-α and CRP were detected by ELISA.
32387236	7	54	theme	inflammatory	1054:1065	arg1	TNF-α					1077:1081	TNF-α	1077:1081	TNF-α	1077:1081	The protein levels of inflammatory mediators TNF-α and CRP were detected by ELISA.
32387236	11	55	theme	taxonomic	1899:1907	arg1	levels					1909:1914	taxonomic levels	1899:1914	taxonomic levels	1899:1914	Moreover, the 16S rRNA gene sequencing analysis demonstrated that HHS treatment during colitis prevented the colitis-associated alteration of colonic microbial community at operational taxonomic unit level, together with the DSS-induced colonic microbiota dysbiosis at taxonomic levels.
32387236	10	56	theme	HHS	1429:1431	arg1	administration					1411:1424	Daily oral administration	1400:1424	Daily oral administration of HHS	1400:1431	RESULTS Daily oral administration of HHS markedly alleviated DSS-induced colitis, as evidenced by decreased colitis disease activity index (DAI) score, reduced colonic inflammation and normalization of colonic vascular hyperpermeability.
32387236	11	57	theme	gene	1653:1656	arg1	sequencing					1658:1667	the 16S rRNA gene sequencing	1640:1667	the 16S rRNA gene sequencing analysis	1640:1676	Moreover, the 16S rRNA gene sequencing analysis demonstrated that HHS treatment during colitis prevented the colitis-associated alteration of colonic microbial community at operational taxonomic unit level, together with the DSS-induced colonic microbiota dysbiosis at taxonomic levels.
32387236	4	58	theme	-induced	690:697	arg1	colitis					699:705	dextran sulphate sodium (DSS)-induced colitis	661:705	dextran sulphate sodium (DSS)-induced colitis	661:705	AIM OF THE STUDY The aim of this study is to investigate the potential effects of HHS in the alleviation of dextran sulphate sodium (DSS)-induced colitis and the alteration of colonic microbiota composition and structure.
32387236	11	59	theme	16S	1644:1646	arg1	sequencing					1658:1667	the 16S rRNA gene sequencing	1640:1667	the 16S rRNA gene sequencing analysis	1640:1676	Moreover, the 16S rRNA gene sequencing analysis demonstrated that HHS treatment during colitis prevented the colitis-associated alteration of colonic microbial community at operational taxonomic unit level, together with the DSS-induced colonic microbiota dysbiosis at taxonomic levels.
32387236	5	60	theme	MATERIALS	775:783	arg1	solution					801:808	MATERIALS AND METHODS HHS solution	775:808	MATERIALS AND METHODS HHS solution	775:808	MATERIALS AND METHODS HHS solution was orally administrated to 5% DSS-challenged rats once a day for 8 days.
32387236	12	61	theme	Bacteroidetes	1993:2005	arg1	ratio					1984:1988	colitis-associated high increased ratio	1950:1988	colitis-associated high increased ratio of Bacteroidetes to Firmicutes	1950:2019	In addition, HHS therapy reduced colitis-associated high increased ratio of Bacteroidetes to Firmicutes to a normal level.
32387236	6	62	theme	ultrastructural	1008:1022	arg1	levels					1024:1029	histomorphometric and ultrastructural levels	986:1029	histomorphometric and ultrastructural levels	986:1029	Colitis clinical symptoms of colitis were collected, together with colonic mucosal damage assessed at histomorphometric and ultrastructural levels.
32387236	7	63	theme	protein	1036:1042	arg1	levels					1044:1049	The protein levels	1032:1049	The protein levels of inflammatory mediators TNF-α and CRP	1032:1089	The protein levels of inflammatory mediators TNF-α and CRP were detected by ELISA.
32387236	6	64	theme	histomorphometric	986:1002	arg1	levels					1024:1029	histomorphometric and ultrastructural levels	986:1029	histomorphometric and ultrastructural levels	986:1029	Colitis clinical symptoms of colitis were collected, together with colonic mucosal damage assessed at histomorphometric and ultrastructural levels.
32387236	10	65	theme	colitis	1500:1506	arg1	index					1525:1529	colitis disease activity index	1500:1529	decreased colitis disease activity index (DAI) score	1490:1541	RESULTS Daily oral administration of HHS markedly alleviated DSS-induced colitis, as evidenced by decreased colitis disease activity index (DAI) score, reduced colonic inflammation and normalization of colonic vascular hyperpermeability.
32387236	10	65	theme	colitis	1500:1506	arg1	DAI					1532:1534	DAI	1532:1534	DAI	1532:1534	RESULTS Daily oral administration of HHS markedly alleviated DSS-induced colitis, as evidenced by decreased colitis disease activity index (DAI) score, reduced colonic inflammation and normalization of colonic vascular hyperpermeability.
32387236	3	66	theme	exact	487:491	arg1	mechanism					493:501	the exact mechanism	483:501	the exact mechanism	483:501	However, scientific evidence of the efficacy and the exact mechanism of HHS against colitis has not yet been reported.
32387236	9	67	theme	rRNA	1321:1324	arg1	gene					1326:1329	the 16S rRNA gene	1313:1329	the 16S rRNA gene	1313:1329	Meanwhile, The effects of the HHS therapy on the colonic microbiota were evaluated by analyzing the V3 and V4 regions of the 16S rRNA gene by Illumina sequencing and multivariate statistical methods.
32387236	12	68	theme	high	1969:1972	arg1	ratio					1984:1988	colitis-associated high increased ratio	1950:1988	colitis-associated high increased ratio of Bacteroidetes to Firmicutes	1950:2019	In addition, HHS therapy reduced colitis-associated high increased ratio of Bacteroidetes to Firmicutes to a normal level.
32387236	1	69	theme	Chinese	165:171	arg1	formula					180:186	a traditional Chinese herbal formula	151:186	a traditional Chinese herbal formula which is firstly documented in the ancient Chinese classic medical work "Pu Ji Ben Shi Fang" in 1132 AD	151:290	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS) is a traditional Chinese herbal formula which is firstly documented in the ancient Chinese classic medical work "Pu Ji Ben Shi Fang" in 1132 AD.
32387236	1	69	theme	Chinese	165:171	arg1	HHS					143:145	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS)	98:146	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS)	98:146	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS) is a traditional Chinese herbal formula which is firstly documented in the ancient Chinese classic medical work "Pu Ji Ben Shi Fang" in 1132 AD.
32387236	0	70	theme	sodium-induced	41:54	arg1	colitis					56:62	dextran sulphate sodium-induced colitis	24:62	dextran sulphate sodium-induced colitis	24:62	Huai hua san alleviates dextran sulphate sodium-induced colitis and modulates colonic microbiota.
32387236	4	71	theme	microbiota	737:746	arg1	composition					748:758	colonic microbiota composition	729:758	colonic microbiota composition	729:758	AIM OF THE STUDY The aim of this study is to investigate the potential effects of HHS in the alleviation of dextran sulphate sodium (DSS)-induced colitis and the alteration of colonic microbiota composition and structure.
32387236	12	72	theme	HHS	1930:1932	arg1	therapy					1934:1940	HHS therapy	1930:1940	HHS therapy	1930:1940	In addition, HHS therapy reduced colitis-associated high increased ratio of Bacteroidetes to Firmicutes to a normal level.
32387236	8	73	theme	Evans	1166:1170	arg1	extravasation					1177:1189	Evans blue extravasation	1166:1189	Evans blue extravasation	1166:1189	The colonic vascular permeability was evaluated by Evans blue extravasation.
32387236	10	74	theme	activity	1516:1523	arg1	index					1525:1529	colitis disease activity index	1500:1529	decreased colitis disease activity index (DAI) score	1490:1541	RESULTS Daily oral administration of HHS markedly alleviated DSS-induced colitis, as evidenced by decreased colitis disease activity index (DAI) score, reduced colonic inflammation and normalization of colonic vascular hyperpermeability.
32387236	10	74	theme	activity	1516:1523	arg1	DAI					1532:1534	DAI	1532:1534	DAI	1532:1534	RESULTS Daily oral administration of HHS markedly alleviated DSS-induced colitis, as evidenced by decreased colitis disease activity index (DAI) score, reduced colonic inflammation and normalization of colonic vascular hyperpermeability.
32387236	9	75	theme	multivariate	1358:1369	arg1	methods					1383:1389	multivariate statistical methods	1358:1389	multivariate statistical methods	1358:1389	Meanwhile, The effects of the HHS therapy on the colonic microbiota were evaluated by analyzing the V3 and V4 regions of the 16S rRNA gene by Illumina sequencing and multivariate statistical methods.
32387236	11	76	theme	unit	1825:1828	arg1	level					1830:1834	operational taxonomic unit level	1803:1834	operational taxonomic unit level	1803:1834	Moreover, the 16S rRNA gene sequencing analysis demonstrated that HHS treatment during colitis prevented the colitis-associated alteration of colonic microbial community at operational taxonomic unit level, together with the DSS-induced colonic microbiota dysbiosis at taxonomic levels.
32387236	13	77	theme	microbial	2109:2117	arg1	dysbiosis					2119:2127	gut microbial dysbiosis	2105:2127	gut microbial dysbiosis	2105:2127	CONCLUSION HHS could attenuate ulcerative colitis and ameliorate gut microbial dysbiosis.
32387236	4	78	theme	study	586:590	arg1	aim					574:576	The aim	570:576	The aim of this study	570:590	AIM OF THE STUDY The aim of this study is to investigate the potential effects of HHS in the alleviation of dextran sulphate sodium (DSS)-induced colitis and the alteration of colonic microbiota composition and structure.
32387236	8	79	theme	colonic	1119:1125	arg1	permeability					1136:1147	The colonic vascular permeability	1115:1147	The colonic vascular permeability	1115:1147	The colonic vascular permeability was evaluated by Evans blue extravasation.
32387236	11	80	theme	community	1790:1798	arg1	alteration					1758:1767	the colitis-associated alteration	1735:1767	the colitis-associated alteration of colonic microbial community at operational taxonomic unit level	1735:1834	Moreover, the 16S rRNA gene sequencing analysis demonstrated that HHS treatment during colitis prevented the colitis-associated alteration of colonic microbial community at operational taxonomic unit level, together with the DSS-induced colonic microbiota dysbiosis at taxonomic levels.
32387236	1	81	theme	hua	134:136	arg1	formula					180:186	a traditional Chinese herbal formula	151:186	a traditional Chinese herbal formula which is firstly documented in the ancient Chinese classic medical work "Pu Ji Ben Shi Fang" in 1132 AD	151:290	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS) is a traditional Chinese herbal formula which is firstly documented in the ancient Chinese classic medical work "Pu Ji Ben Shi Fang" in 1132 AD.
32387236	1	81	theme	hua	134:136	arg1	HHS					143:145	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS)	98:146	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS)	98:146	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS) is a traditional Chinese herbal formula which is firstly documented in the ancient Chinese classic medical work "Pu Ji Ben Shi Fang" in 1132 AD.
32387236	9	82	from	effects	1207:1213	arg1	microbiota					1249:1258	the colonic microbiota	1237:1258	the colonic microbiota	1237:1258	Meanwhile, The effects of the HHS therapy on the colonic microbiota were evaluated by analyzing the V3 and V4 regions of the 16S rRNA gene by Illumina sequencing and multivariate statistical methods.
32387236	11	83	theme	colonic	1772:1778	arg1	community					1790:1798	colonic microbial community	1772:1798	colonic microbial community	1772:1798	Moreover, the 16S rRNA gene sequencing analysis demonstrated that HHS treatment during colitis prevented the colitis-associated alteration of colonic microbial community at operational taxonomic unit level, together with the DSS-induced colonic microbiota dysbiosis at taxonomic levels.
32387236	4	84	dep	AIM	553:555	arg1	is					592:593	is	592:593	is to investigate the potential effects of HHS in the alleviation of dextran sulphate sodium (DSS)-induced colitis and the alteration of colonic microbiota composition and structure	592:772	AIM OF THE STUDY The aim of this study is to investigate the potential effects of HHS in the alleviation of dextran sulphate sodium (DSS)-induced colitis and the alteration of colonic microbiota composition and structure.
32387236	5	85	theme	5	838:838	arg1	%					839:839	%	839:839	%	839:839	MATERIALS AND METHODS HHS solution was orally administrated to 5% DSS-challenged rats once a day for 8 days.
32387236	4	86	theme	HHS	635:637	arg1	effects					624:630	the potential effects	610:630	the potential effects of HHS in the alleviation of dextran sulphate sodium (DSS)-induced colitis and the alteration of colonic microbiota composition and structure	610:772	AIM OF THE STUDY The aim of this study is to investigate the potential effects of HHS in the alleviation of dextran sulphate sodium (DSS)-induced colitis and the alteration of colonic microbiota composition and structure.
32387236	5	87	theme	HHS	797:799	arg1	solution					801:808	MATERIALS AND METHODS HHS solution	775:808	MATERIALS AND METHODS HHS solution	775:808	MATERIALS AND METHODS HHS solution was orally administrated to 5% DSS-challenged rats once a day for 8 days.
32387236	6	88	theme	colitis	913:919	arg1	symptoms					901:908	Colitis clinical symptoms	884:908	Colitis clinical symptoms of colitis	884:919	Colitis clinical symptoms of colitis were collected, together with colonic mucosal damage assessed at histomorphometric and ultrastructural levels.
32387236	11	89	from	level	1830:1834	arg1	alteration					1758:1767	the colitis-associated alteration	1735:1767	the colitis-associated alteration of colonic microbial community at operational taxonomic unit level	1735:1834	Moreover, the 16S rRNA gene sequencing analysis demonstrated that HHS treatment during colitis prevented the colitis-associated alteration of colonic microbial community at operational taxonomic unit level, together with the DSS-induced colonic microbiota dysbiosis at taxonomic levels.
32387236	6	90	theme	Colitis	884:890	arg1	symptoms					901:908	Colitis clinical symptoms	884:908	Colitis clinical symptoms of colitis	884:919	Colitis clinical symptoms of colitis were collected, together with colonic mucosal damage assessed at histomorphometric and ultrastructural levels.
32387236	11	91	theme	operational	1803:1813	arg1	unit					1825:1828	operational taxonomic unit	1803:1828	operational taxonomic unit level	1803:1834	Moreover, the 16S rRNA gene sequencing analysis demonstrated that HHS treatment during colitis prevented the colitis-associated alteration of colonic microbial community at operational taxonomic unit level, together with the DSS-induced colonic microbiota dysbiosis at taxonomic levels.
32387236	9	92	theme	therapy	1226:1232	arg1	Meanwhile					1192:1200	Meanwhile	1192:1200	Meanwhile	1192:1200	Meanwhile, The effects of the HHS therapy on the colonic microbiota were evaluated by analyzing the V3 and V4 regions of the 16S rRNA gene by Illumina sequencing and multivariate statistical methods.
32387236	9	92	theme	therapy	1226:1232	arg1	effects					1207:1213	The effects	1203:1213	The effects of the HHS therapy on the colonic microbiota	1203:1258	Meanwhile, The effects of the HHS therapy on the colonic microbiota were evaluated by analyzing the V3 and V4 regions of the 16S rRNA gene by Illumina sequencing and multivariate statistical methods.
32387236	2	93	theme	lower	337:341	arg1	hematochezia					396:407	hematochezia	396:407	hematochezia	396:407	It has been widely used in the treatment of lower gastrointestinal disorders such as acute colitis and hematochezia for more than 800 years.
32387236	2	93	theme	lower	337:341	arg1	colitis					384:390	acute colitis	378:390	acute colitis	378:390	It has been widely used in the treatment of lower gastrointestinal disorders such as acute colitis and hematochezia for more than 800 years.
32387236	2	93	theme	lower	337:341	arg1	disorders					360:368	lower gastrointestinal disorders	337:368	lower gastrointestinal disorders such as acute colitis and hematochezia	337:407	It has been widely used in the treatment of lower gastrointestinal disorders such as acute colitis and hematochezia for more than 800 years.
32387236	0	94	theme	hua	5:7	arg1	san					9:11	Huai hua san	0:11	Huai hua san	0:11	Huai hua san alleviates dextran sulphate sodium-induced colitis and modulates colonic microbiota.
32387236	5	95	theme	DSS-challenged	841:854	arg1	rats					856:859	5% DSS-challenged rats	838:859	5% DSS-challenged rats	838:859	MATERIALS AND METHODS HHS solution was orally administrated to 5% DSS-challenged rats once a day for 8 days.
32387236	10	96	theme	reduced	1544:1550	arg1	inflammation					1560:1571	reduced colonic inflammation	1544:1571	reduced colonic inflammation	1544:1571	RESULTS Daily oral administration of HHS markedly alleviated DSS-induced colitis, as evidenced by decreased colitis disease activity index (DAI) score, reduced colonic inflammation and normalization of colonic vascular hyperpermeability.
32387236	1	97	theme	RELEVANCE	119:127	arg1	formula					180:186	a traditional Chinese herbal formula	151:186	a traditional Chinese herbal formula which is firstly documented in the ancient Chinese classic medical work "Pu Ji Ben Shi Fang" in 1132 AD	151:290	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS) is a traditional Chinese herbal formula which is firstly documented in the ancient Chinese classic medical work "Pu Ji Ben Shi Fang" in 1132 AD.
32387236	1	97	theme	RELEVANCE	119:127	arg1	HHS					143:145	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS)	98:146	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS)	98:146	ETHNOPHARMACOLOGICAL RELEVANCE Huai hua san (HHS) is a traditional Chinese herbal formula which is firstly documented in the ancient Chinese classic medical work "Pu Ji Ben Shi Fang" in 1132 AD.
32387236	6	98	theme	colonic	951:957	arg1	damage					967:972	colonic mucosal damage	951:972	colonic mucosal damage assessed at histomorphometric and ultrastructural levels	951:1029	Colitis clinical symptoms of colitis were collected, together with colonic mucosal damage assessed at histomorphometric and ultrastructural levels.
32387236	10	99	theme	oral	1406:1409	arg1	administration					1411:1424	Daily oral administration	1400:1424	Daily oral administration of HHS	1400:1431	RESULTS Daily oral administration of HHS markedly alleviated DSS-induced colitis, as evidenced by decreased colitis disease activity index (DAI) score, reduced colonic inflammation and normalization of colonic vascular hyperpermeability.
32387236	10	100	theme	colonic	1594:1600	arg1	hyperpermeability					1611:1627	colonic vascular hyperpermeability	1594:1627	colonic vascular hyperpermeability	1594:1627	RESULTS Daily oral administration of HHS markedly alleviated DSS-induced colitis, as evidenced by decreased colitis disease activity index (DAI) score, reduced colonic inflammation and normalization of colonic vascular hyperpermeability.
32387236	7	101	theme	mediators	1067:1075	arg1	levels					1044:1049	The protein levels	1032:1049	The protein levels of inflammatory mediators TNF-α and CRP	1032:1089	The protein levels of inflammatory mediators TNF-α and CRP were detected by ELISA.
32387236	4	102	from	effects	624:630	arg1	alteration					715:724	the alteration	711:724	the alteration of colonic microbiota composition and structure	711:772	AIM OF THE STUDY The aim of this study is to investigate the potential effects of HHS in the alleviation of dextran sulphate sodium (DSS)-induced colitis and the alteration of colonic microbiota composition and structure.
32387236	4	102	from	effects	624:630	arg1	alleviation					646:656	the alleviation	642:656	the alleviation of dextran sulphate sodium (DSS)-induced colitis	642:705	AIM OF THE STUDY The aim of this study is to investigate the potential effects of HHS in the alleviation of dextran sulphate sodium (DSS)-induced colitis and the alteration of colonic microbiota composition and structure.
32387236	11	103	theme	colonic	1867:1873	arg1	dysbiosis					1886:1894	the DSS-induced colonic microbiota dysbiosis	1851:1894	the DSS-induced colonic microbiota dysbiosis at taxonomic levels	1851:1914	Moreover, the 16S rRNA gene sequencing analysis demonstrated that HHS treatment during colitis prevented the colitis-associated alteration of colonic microbial community at operational taxonomic unit level, together with the DSS-induced colonic microbiota dysbiosis at taxonomic levels.
32387236	2	104	theme	disorders	360:368	arg1	treatment					324:332	the treatment	320:332	the treatment of lower gastrointestinal disorders such as acute colitis and hematochezia for more than 800 years	320:431	It has been widely used in the treatment of lower gastrointestinal disorders such as acute colitis and hematochezia for more than 800 years.
32387236	11	105	theme	rRNA	1648:1651	arg1	sequencing					1658:1667	the 16S rRNA gene sequencing	1640:1667	the 16S rRNA gene sequencing analysis	1640:1676	Moreover, the 16S rRNA gene sequencing analysis demonstrated that HHS treatment during colitis prevented the colitis-associated alteration of colonic microbial community at operational taxonomic unit level, together with the DSS-induced colonic microbiota dysbiosis at taxonomic levels.
32387236	3	106	theme	scientific	443:452	arg1	evidence					454:461	scientific evidence	443:461	scientific evidence of the efficacy and the exact mechanism of HHS against colitis	443:524	However, scientific evidence of the efficacy and the exact mechanism of HHS against colitis has not yet been reported.
32387236	9	107	theme	V3	1292:1293	arg1	regions					1302:1308	the V3 and V4 regions	1288:1308	the V3 and V4 regions of the 16S rRNA gene	1288:1329	Meanwhile, The effects of the HHS therapy on the colonic microbiota were evaluated by analyzing the V3 and V4 regions of the 16S rRNA gene by Illumina sequencing and multivariate statistical methods.
32387236	10	108	theme	hyperpermeability	1611:1627	arg1	inflammation					1560:1571	reduced colonic inflammation	1544:1571	reduced colonic inflammation	1544:1571	RESULTS Daily oral administration of HHS markedly alleviated DSS-induced colitis, as evidenced by decreased colitis disease activity index (DAI) score, reduced colonic inflammation and normalization of colonic vascular hyperpermeability.
32387236	10	108	theme	hyperpermeability	1611:1627	arg1	score					1537:1541	decreased colitis disease activity index (DAI) score	1490:1541	decreased colitis disease activity index (DAI) score	1490:1541	RESULTS Daily oral administration of HHS markedly alleviated DSS-induced colitis, as evidenced by decreased colitis disease activity index (DAI) score, reduced colonic inflammation and normalization of colonic vascular hyperpermeability.
32387236	10	108	theme	hyperpermeability	1611:1627	arg1	normalization					1577:1589	normalization	1577:1589	normalization of colonic vascular hyperpermeability	1577:1627	RESULTS Daily oral administration of HHS markedly alleviated DSS-induced colitis, as evidenced by decreased colitis disease activity index (DAI) score, reduced colonic inflammation and normalization of colonic vascular hyperpermeability.
32387236	9	109	theme	V4	1299:1300	arg1	regions					1302:1308	the V3 and V4 regions	1288:1308	the V3 and V4 regions of the 16S rRNA gene	1288:1329	Meanwhile, The effects of the HHS therapy on the colonic microbiota were evaluated by analyzing the V3 and V4 regions of the 16S rRNA gene by Illumina sequencing and multivariate statistical methods.
32387236	11	110	theme	microbiota	1875:1884	arg1	dysbiosis					1886:1894	the DSS-induced colonic microbiota dysbiosis	1851:1894	the DSS-induced colonic microbiota dysbiosis at taxonomic levels	1851:1914	Moreover, the 16S rRNA gene sequencing analysis demonstrated that HHS treatment during colitis prevented the colitis-associated alteration of colonic microbial community at operational taxonomic unit level, together with the DSS-induced colonic microbiota dysbiosis at taxonomic levels.
32387236	4	111	theme	colitis	699:705	arg1	alteration					715:724	the alteration	711:724	the alteration of colonic microbiota composition and structure	711:772	AIM OF THE STUDY The aim of this study is to investigate the potential effects of HHS in the alleviation of dextran sulphate sodium (DSS)-induced colitis and the alteration of colonic microbiota composition and structure.
32387236	4	111	theme	colitis	699:705	arg1	alleviation					646:656	the alleviation	642:656	the alleviation of dextran sulphate sodium (DSS)-induced colitis	642:705	AIM OF THE STUDY The aim of this study is to investigate the potential effects of HHS in the alleviation of dextran sulphate sodium (DSS)-induced colitis and the alteration of colonic microbiota composition and structure.
32387236	3	112	theme	efficacy	470:477	arg1	evidence					454:461	scientific evidence	443:461	scientific evidence of the efficacy and the exact mechanism of HHS against colitis	443:524	However, scientific evidence of the efficacy and the exact mechanism of HHS against colitis has not yet been reported.
32375943	2	0	theme	endospore-forming	102:118	arg1	strain					120:125	A Gram-stain-positive, motile, rod-shaped and endospore-forming strain	56:125	A Gram-stain-positive, motile, rod-shaped and endospore-forming strain	56:125	A Gram-stain-positive, motile, rod-shaped and endospore-forming strain, SYSU K30002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-west China.
32375943	2	0	theme	endospore-forming	102:118	arg1	K30002T					133:139	K30002T	133:139	K30002T	133:139	A Gram-stain-positive, motile, rod-shaped and endospore-forming strain, SYSU K30002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-west China.
32375943	5	1	theme	K30002T	491:497	arg1	ribose					504:509	ribose	504:509	ribose	504:509	The cell-wall sugars of SYSU K30002T were ribose, galactose and mannose, and MK-7 was the menaquinone.
32375943	5	1	theme	K30002T	491:497	arg1	sugars					476:481	The cell-wall sugars	462:481	The cell-wall sugars of SYSU K30002T	462:497	The cell-wall sugars of SYSU K30002T were ribose, galactose and mannose, and MK-7 was the menaquinone.
32375943	8	2	theme	DNA	802:804	arg1	content					779:785	The G+C content	771:785	The G+C content of the genomic DNA	771:804	The G+C content of the genomic DNA was 36.1 mol%.
32375943	8	2	theme	DNA	802:804	arg1	%					818:818	36.1 mol%	810:818	36.1 mol%	810:818	The G+C content of the genomic DNA was 36.1 mol%.
32375943	6	3	dep	iso-C15 	592:599	arg1	 0					638:639	 0	638:639	 0	638:639	The major fatty acids were iso-C15 : 0, C16 : 1 ω7c alcohol and iso-C16 : 0.
32375943	6	3	dep	iso-C15 	592:599	arg1	ω7c					613:615	 1 ω7c alcohol and iso-C16 	610:636	ω7c	613:615	The major fatty acids were iso-C15 : 0, C16 : 1 ω7c alcohol and iso-C16 : 0.
32375943	6	3	dep	iso-C15 	592:599	arg1	iso-C16 					629:636	iso-C16 	629:636	iso-C16 	629:636	The major fatty acids were iso-C15 : 0, C16 : 1 ω7c alcohol and iso-C16 : 0.
32375943	6	3	dep	iso-C15 	592:599	arg1	C16 					605:608	C16 	605:608	C16 	605:608	The major fatty acids were iso-C15 : 0, C16 : 1 ω7c alcohol and iso-C16 : 0.
32375943	2	4	theme	rod-shaped	87:96	arg1	strain					120:125	A Gram-stain-positive, motile, rod-shaped and endospore-forming strain	56:125	A Gram-stain-positive, motile, rod-shaped and endospore-forming strain	56:125	A Gram-stain-positive, motile, rod-shaped and endospore-forming strain, SYSU K30002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-west China.
32375943	2	4	theme	rod-shaped	87:96	arg1	K30002T					133:139	K30002T	133:139	K30002T	133:139	A Gram-stain-positive, motile, rod-shaped and endospore-forming strain, SYSU K30002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-west China.
32375943	3	5	from	optimum	377:383	arg1	absence					392:398	the absence	388:398	the absence of NaCl	388:406	SYSU K30002T grew at 28-40 °C (optimum, 37 °C), at pH 5.0-8.0 (optimum, pH 7.0) and in the presence of 0-4 % (w/v) NaCl (optimum in the absence of NaCl).
32375943	3	6	theme	NaCl	403:406	arg1	absence					392:398	the absence	388:398	the absence of NaCl	388:406	SYSU K30002T grew at 28-40 °C (optimum, 37 °C), at pH 5.0-8.0 (optimum, pH 7.0) and in the presence of 0-4 % (w/v) NaCl (optimum in the absence of NaCl).
32375943	10	7	theme	genome	1011:1016	arg1	comparisons					1018:1028	genome comparisons	1011:1028	genome comparisons	1011:1028	Based on phenotypic, chemotaxonomic and genome comparisons, strain SYSU K30002T represents a novel species of the genus Lysinibacillus, for which the name Lysinibacillusantri sp.
32375943	9	8	theme	species	950:956	arg1	delineation					958:968	species delineation	950:968	species delineation	950:968	The average nucleotide identity values between SYSU K30002T and its closest relatives were below the cut-off level (95-96 %) for species delineation.
32375943	8	9	theme	36.1 mol	810:817	arg1	content					779:785	The G+C content	771:785	The G+C content of the genomic DNA	771:804	The G+C content of the genomic DNA was 36.1 mol%.
32375943	8	9	theme	36.1 mol	810:817	arg1	%					818:818	36.1 mol%	810:818	36.1 mol%	810:818	The G+C content of the genomic DNA was 36.1 mol%.
32375943	2	10	theme	south-west	238:247	arg1	county					212:217	county	212:217	county	212:217	A Gram-stain-positive, motile, rod-shaped and endospore-forming strain, SYSU K30002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-west China.
32375943	2	10	theme	south-west	238:247	arg1	China					249:253	south-west China	238:253	south-west China	238:253	A Gram-stain-positive, motile, rod-shaped and endospore-forming strain, SYSU K30002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-west China.
32375943	7	11	theme	polar	646:650	arg1	diphosphatidylglycerol					664:685	diphosphatidylglycerol	664:685	diphosphatidylglycerol	664:685	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified phospholipids.
32375943	7	11	theme	polar	646:650	arg1	lipids					652:657	The polar lipids	642:657	The polar lipids	642:657	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified phospholipids.
32375943	9	12	theme	closest	889:895	arg1	relatives					897:905	its closest relatives	885:905	its closest relatives	885:905	The average nucleotide identity values between SYSU K30002T and its closest relatives were below the cut-off level (95-96 %) for species delineation.
32375943	3	13	dep	optimum	319:325	arg1	pH					328:329	pH 7.0	328:333	pH 7.0	328:333	SYSU K30002T grew at 28-40 °C (optimum, 37 °C), at pH 5.0-8.0 (optimum, pH 7.0) and in the presence of 0-4 % (w/v) NaCl (optimum in the absence of NaCl).
32375943	12	14	theme	33955T=CGMCC	1207:1218	arg1	1.13504T					1220:1227	=KCTC 33955T=CGMCC 1.13504T	1201:1227	=KCTC 33955T=CGMCC 1.13504T	1201:1227	The type strain is SYSU K30002T (=KCTC 33955T=CGMCC 1.13504T).
32375943	12	14	theme	33955T=CGMCC	1207:1218	arg1	K30002T					1192:1198	SYSU K30002T	1187:1198	SYSU K30002T (=KCTC 33955T=CGMCC 1.13504T)	1187:1228	The type strain is SYSU K30002T (=KCTC 33955T=CGMCC 1.13504T).
32375943	10	15	theme	Lysinibacillus	1091:1104	arg1	species					1070:1076	a novel species	1062:1076	a novel species	1062:1076	Based on phenotypic, chemotaxonomic and genome comparisons, strain SYSU K30002T represents a novel species of the genus Lysinibacillus, for which the name Lysinibacillusantri sp.
32375943	3	16	dep	NaCl	371:374	arg1	optimum					377:383	optimum	377:383	optimum	377:383	SYSU K30002T grew at 28-40 °C (optimum, 37 °C), at pH 5.0-8.0 (optimum, pH 7.0) and in the presence of 0-4 % (w/v) NaCl (optimum in the absence of NaCl).
32375943	10	17	theme	genus	1085:1089	arg1	Lysinibacillus					1091:1104	the genus Lysinibacillus	1081:1104	the genus Lysinibacillus	1081:1104	Based on phenotypic, chemotaxonomic and genome comparisons, strain SYSU K30002T represents a novel species of the genus Lysinibacillus, for which the name Lysinibacillusantri sp.
32375943	0	18	theme	antri	15:19	arg1	sp					21:22	Lysinibacillus antri sp	0:22	Lysinibacillus antri sp.	0:23	Lysinibacillus antri sp.
32375943	2	19	from	cave	197:200	arg1	county					212:217	county	212:217	county	212:217	A Gram-stain-positive, motile, rod-shaped and endospore-forming strain, SYSU K30002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-west China.
32375943	2	19	from	cave	197:200	arg1	province					228:235	province	228:235	province	228:235	A Gram-stain-positive, motile, rod-shaped and endospore-forming strain, SYSU K30002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-west China.
32375943	2	19	from	cave	197:200	arg1	China					249:253	south-west China	238:253	south-west China	238:253	A Gram-stain-positive, motile, rod-shaped and endospore-forming strain, SYSU K30002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-west China.
32375943	12	20	theme	=KCTC	1201:1205	arg1	1.13504T					1220:1227	=KCTC 33955T=CGMCC 1.13504T	1201:1227	=KCTC 33955T=CGMCC 1.13504T	1201:1227	The type strain is SYSU K30002T (=KCTC 33955T=CGMCC 1.13504T).
32375943	12	20	theme	=KCTC	1201:1205	arg1	K30002T					1192:1198	SYSU K30002T	1187:1198	SYSU K30002T (=KCTC 33955T=CGMCC 1.13504T)	1187:1228	The type strain is SYSU K30002T (=KCTC 33955T=CGMCC 1.13504T).
32375943	0	21	theme	Lysinibacillus	0:13	arg1	sp					21:22	Lysinibacillus antri sp	0:22	Lysinibacillus antri sp.	0:23	Lysinibacillus antri sp.
32375943	7	22	theme	unidentified	743:754	arg1	phospholipids					756:768	two unidentified phospholipids	739:768	two unidentified phospholipids	739:768	The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified phospholipids.
32375943	5	23	theme	cell-wall	466:474	arg1	ribose					504:509	ribose	504:509	ribose	504:509	The cell-wall sugars of SYSU K30002T were ribose, galactose and mannose, and MK-7 was the menaquinone.
32375943	5	23	theme	cell-wall	466:474	arg1	sugars					476:481	The cell-wall sugars	462:481	The cell-wall sugars of SYSU K30002T	462:497	The cell-wall sugars of SYSU K30002T were ribose, galactose and mannose, and MK-7 was the menaquinone.
32375943	10	24	theme	name	1121:1124	arg1	sp					1146:1147	the name Lysinibacillusantri sp	1117:1147	the name Lysinibacillusantri sp	1117:1147	Based on phenotypic, chemotaxonomic and genome comparisons, strain SYSU K30002T represents a novel species of the genus Lysinibacillus, for which the name Lysinibacillusantri sp.
32375943	12	25	theme	SYSU	1187:1190	arg1	1.13504T					1220:1227	=KCTC 33955T=CGMCC 1.13504T	1201:1227	=KCTC 33955T=CGMCC 1.13504T	1201:1227	The type strain is SYSU K30002T (=KCTC 33955T=CGMCC 1.13504T).
32375943	12	25	theme	SYSU	1187:1190	arg1	strain					1177:1182	The type strain	1168:1182	The type strain	1168:1182	The type strain is SYSU K30002T (=KCTC 33955T=CGMCC 1.13504T).
32375943	12	25	theme	SYSU	1187:1190	arg1	K30002T					1192:1198	SYSU K30002T	1187:1198	SYSU K30002T (=KCTC 33955T=CGMCC 1.13504T)	1187:1228	The type strain is SYSU K30002T (=KCTC 33955T=CGMCC 1.13504T).
32375943	9	26	theme	cut-off	922:928	arg1	level					930:934	the cut-off level	918:934	the cut-off level (95-96 %)	918:944	The average nucleotide identity values between SYSU K30002T and its closest relatives were below the cut-off level (95-96 %) for species delineation.
32375943	9	26	theme	cut-off	922:928	arg1	%					943:943	95-96 %	937:943	95-96 %	937:943	The average nucleotide identity values between SYSU K30002T and its closest relatives were below the cut-off level (95-96 %) for species delineation.
32375943	3	27	theme	0-4 	359:362	arg1	NaCl					371:374	0-4 % (w/v) NaCl	359:374	0-4 % (w/v) NaCl (optimum in the absence of NaCl)	359:407	SYSU K30002T grew at 28-40 °C (optimum, 37 °C), at pH 5.0-8.0 (optimum, pH 7.0) and in the presence of 0-4 % (w/v) NaCl (optimum in the absence of NaCl).
32375943	12	28	theme	type	1172:1175	arg1	strain					1177:1182	The type strain	1168:1182	The type strain	1168:1182	The type strain is SYSU K30002T (=KCTC 33955T=CGMCC 1.13504T).
32375943	12	28	theme	type	1172:1175	arg1	K30002T					1192:1198	SYSU K30002T	1187:1198	SYSU K30002T (=KCTC 33955T=CGMCC 1.13504T)	1187:1228	The type strain is SYSU K30002T (=KCTC 33955T=CGMCC 1.13504T).
32375943	4	29	theme	peptidoglycan	424:436	arg1	A4α					447:449	A4α	447:449	A4α (Lys-Asp)	447:459	The cell-wall peptidoglycan type was A4α (Lys-Asp).
32375943	4	29	theme	peptidoglycan	424:436	arg1	type					438:441	The cell-wall peptidoglycan type	410:441	The cell-wall peptidoglycan type	410:441	The cell-wall peptidoglycan type was A4α (Lys-Asp).
32375943	3	30	theme	%	363:363	arg1	NaCl					371:374	0-4 % (w/v) NaCl	359:374	0-4 % (w/v) NaCl (optimum in the absence of NaCl)	359:407	SYSU K30002T grew at 28-40 °C (optimum, 37 °C), at pH 5.0-8.0 (optimum, pH 7.0) and in the presence of 0-4 % (w/v) NaCl (optimum in the absence of NaCl).
32375943	4	31	theme	cell-wall	414:422	arg1	A4α					447:449	A4α	447:449	A4α (Lys-Asp)	447:459	The cell-wall peptidoglycan type was A4α (Lys-Asp).
32375943	4	31	theme	cell-wall	414:422	arg1	type					438:441	The cell-wall peptidoglycan type	410:441	The cell-wall peptidoglycan type	410:441	The cell-wall peptidoglycan type was A4α (Lys-Asp).
32375943	10	32	theme	novel	1064:1068	arg1	species					1070:1076	a novel species	1062:1076	a novel species	1062:1076	Based on phenotypic, chemotaxonomic and genome comparisons, strain SYSU K30002T represents a novel species of the genus Lysinibacillus, for which the name Lysinibacillusantri sp.
32375943	6	33	dep	ω7c	613:615	arg1	alcohol					617:623	alcohol	617:623	alcohol	617:623	The major fatty acids were iso-C15 : 0, C16 : 1 ω7c alcohol and iso-C16 : 0.
32375943	3	34	dep	optimum	287:293	arg1	37 °C					296:300	37 °C	296:300	37 °C	296:300	SYSU K30002T grew at 28-40 °C (optimum, 37 °C), at pH 5.0-8.0 (optimum, pH 7.0) and in the presence of 0-4 % (w/v) NaCl (optimum in the absence of NaCl).
32375943	2	35	attach	isolated	146:153	arg1	sample					167:172	a soil sample	160:172	a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-west China	160:253	A Gram-stain-positive, motile, rod-shaped and endospore-forming strain, SYSU K30002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-west China.
32375943	2	35	attach	isolated	146:153	arg2	K30002T					133:139	K30002T	133:139	K30002T	133:139	A Gram-stain-positive, motile, rod-shaped and endospore-forming strain, SYSU K30002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-west China.
32375943	2	35	attach	isolated	146:153	arg2	strain					120:125	A Gram-stain-positive, motile, rod-shaped and endospore-forming strain	56:125	A Gram-stain-positive, motile, rod-shaped and endospore-forming strain	56:125	A Gram-stain-positive, motile, rod-shaped and endospore-forming strain, SYSU K30002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-west China.
32375943	2	36	theme	motile	79:84	arg1	strain					120:125	A Gram-stain-positive, motile, rod-shaped and endospore-forming strain	56:125	A Gram-stain-positive, motile, rod-shaped and endospore-forming strain	56:125	A Gram-stain-positive, motile, rod-shaped and endospore-forming strain, SYSU K30002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-west China.
32375943	2	36	theme	motile	79:84	arg1	K30002T					133:139	K30002T	133:139	K30002T	133:139	A Gram-stain-positive, motile, rod-shaped and endospore-forming strain, SYSU K30002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-west China.
32375943	8	37	theme	genomic	794:800	arg1	DNA					802:804	the genomic DNA	790:804	the genomic DNA	790:804	The G+C content of the genomic DNA was 36.1 mol%.
32375943	2	38	theme	karst	191:195	arg1	cave					197:200	a karst cave	189:200	a karst cave in Xingyi county, Guizhou province, south-west China	189:253	A Gram-stain-positive, motile, rod-shaped and endospore-forming strain, SYSU K30002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-west China.
32375943	9	39	theme	average	825:831	arg1	values					853:858	The average nucleotide identity values	821:858	The average nucleotide identity values between SYSU K30002T and its closest relatives	821:905	The average nucleotide identity values between SYSU K30002T and its closest relatives were below the cut-off level (95-96 %) for species delineation.
32375943	3	40	theme	NaCl	371:374	arg1	presence					347:354	the presence	343:354	the presence of 0-4 % (w/v) NaCl (optimum in the absence of NaCl)	343:407	SYSU K30002T grew at 28-40 °C (optimum, 37 °C), at pH 5.0-8.0 (optimum, pH 7.0) and in the presence of 0-4 % (w/v) NaCl (optimum in the absence of NaCl).
32375943	9	41	theme	nucleotide	833:842	arg1	values					853:858	The average nucleotide identity values	821:858	The average nucleotide identity values between SYSU K30002T and its closest relatives	821:905	The average nucleotide identity values between SYSU K30002T and its closest relatives were below the cut-off level (95-96 %) for species delineation.
32375943	2	42	theme	Gram-stain-positive	58:76	arg1	strain					120:125	A Gram-stain-positive, motile, rod-shaped and endospore-forming strain	56:125	A Gram-stain-positive, motile, rod-shaped and endospore-forming strain	56:125	A Gram-stain-positive, motile, rod-shaped and endospore-forming strain, SYSU K30002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-west China.
32375943	2	42	theme	Gram-stain-positive	58:76	arg1	K30002T					133:139	K30002T	133:139	K30002T	133:139	A Gram-stain-positive, motile, rod-shaped and endospore-forming strain, SYSU K30002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-west China.
32375943	6	43	theme	fatty	575:579	arg1	acids					581:585	The major fatty acids	565:585	The major fatty acids	565:585	The major fatty acids were iso-C15 : 0, C16 : 1 ω7c alcohol and iso-C16 : 0.
32375943	6	43	theme	fatty	575:579	arg1	iso-C15 					592:599	iso-C15 	592:599	iso-C15 	592:599	The major fatty acids were iso-C15 : 0, C16 : 1 ω7c alcohol and iso-C16 : 0.
32375943	9	44	theme	identity	844:851	arg1	values					853:858	The average nucleotide identity values	821:858	The average nucleotide identity values between SYSU K30002T and its closest relatives	821:905	The average nucleotide identity values between SYSU K30002T and its closest relatives were below the cut-off level (95-96 %) for species delineation.
32375943	10	45	theme	Lysinibacillusantri	1126:1144	arg1	sp					1146:1147	the name Lysinibacillusantri sp	1117:1147	the name Lysinibacillusantri sp	1117:1147	Based on phenotypic, chemotaxonomic and genome comparisons, strain SYSU K30002T represents a novel species of the genus Lysinibacillus, for which the name Lysinibacillusantri sp.
32375943	1	46	theme	cave	45:48	arg1	soil					50:53	cave soil	45:53	cave soil	45:53	nov., isolated from cave soil.
32375943	6	47	theme	major	569:573	arg1	acids					581:585	The major fatty acids	565:585	The major fatty acids	565:585	The major fatty acids were iso-C15 : 0, C16 : 1 ω7c alcohol and iso-C16 : 0.
32375943	6	47	theme	major	569:573	arg1	iso-C15 					592:599	iso-C15 	592:599	iso-C15 	592:599	The major fatty acids were iso-C15 : 0, C16 : 1 ω7c alcohol and iso-C16 : 0.
32375943	3	48	theme	w/v	366:368	arg1	NaCl					371:374	0-4 % (w/v) NaCl	359:374	0-4 % (w/v) NaCl (optimum in the absence of NaCl)	359:407	SYSU K30002T grew at 28-40 °C (optimum, 37 °C), at pH 5.0-8.0 (optimum, pH 7.0) and in the presence of 0-4 % (w/v) NaCl (optimum in the absence of NaCl).
32375943	10	49	theme	SYSU	1038:1041	arg1	K30002T					1043:1049	strain SYSU K30002T	1031:1049	strain SYSU K30002T	1031:1049	Based on phenotypic, chemotaxonomic and genome comparisons, strain SYSU K30002T represents a novel species of the genus Lysinibacillus, for which the name Lysinibacillusantri sp.
32375943	2	50	theme	soil	162:165	arg1	sample					167:172	a soil sample	160:172	a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-west China	160:253	A Gram-stain-positive, motile, rod-shaped and endospore-forming strain, SYSU K30002T, was isolated from a soil sample collected from a karst cave in Xingyi county, Guizhou province, south-west China.
32375943	8	51	theme	G+C	775:777	arg1	content					779:785	The G+C content	771:785	The G+C content of the genomic DNA	771:804	The G+C content of the genomic DNA was 36.1 mol%.
32375943	8	51	theme	G+C	775:777	arg1	%					818:818	36.1 mol%	810:818	36.1 mol%	810:818	The G+C content of the genomic DNA was 36.1 mol%.
32375943	10	52	theme	strain	1031:1036	arg1	K30002T					1043:1049	strain SYSU K30002T	1031:1049	strain SYSU K30002T	1031:1049	Based on phenotypic, chemotaxonomic and genome comparisons, strain SYSU K30002T represents a novel species of the genus Lysinibacillus, for which the name Lysinibacillusantri sp.
32375943	3	53	from	absence	392:398	arg1	optimum					377:383	optimum	377:383	optimum	377:383	SYSU K30002T grew at 28-40 °C (optimum, 37 °C), at pH 5.0-8.0 (optimum, pH 7.0) and in the presence of 0-4 % (w/v) NaCl (optimum in the absence of NaCl).
32183692	4	0	theme	rDNA	817:820	arg1	region					803:808	the V3-V4 region	793:808	the V3-V4 region of 16S rDNA	793:820	RESULTS The bacterial communities in honeybee midguts and hindguts before winter and after bees were fed honey, sucrose, and high-fructose syrup as winter-food were determined by targeting the V3-V4 region of 16S rDNA using the Illumina MiSeq platform.
32183692	4	1	theme	MiSeq	841:845	arg1	platform					847:854	the Illumina MiSeq platform	828:854	the Illumina MiSeq platform	828:854	RESULTS The bacterial communities in honeybee midguts and hindguts before winter and after bees were fed honey, sucrose, and high-fructose syrup as winter-food were determined by targeting the V3-V4 region of 16S rDNA using the Illumina MiSeq platform.
32183692	8	2	theme	honey	1432:1436	arg1	groups					1456:1461	the honey and high-fructose groups	1428:1461	groups	1456:1461	In the midgut, microbial diversity of the sucrose group was higher than that of the honey and high-fructose syrup groups, but in the hindgut, microbial diversity of the honey and high-fructose groups was higher than that in the sucrose group.
32183692	6	3	theme	sugar	1140:1144	arg1	position					1158:1165	spatial position	1150:1165	spatial position in the gut	1150:1176	The dominant taxa were conserved and not affected by season, type of overwintering sugar, or spatial position in the gut.
32183692	6	3	theme	sugar	1140:1144	arg1	type					1118:1121	type	1118:1121	type of overwintering sugar	1118:1144	The dominant taxa were conserved and not affected by season, type of overwintering sugar, or spatial position in the gut.
32183692	6	3	theme	sugar	1140:1144	arg1	season					1110:1115	season	1110:1115	season	1110:1115	The dominant taxa were conserved and not affected by season, type of overwintering sugar, or spatial position in the gut.
32183692	8	4	theme	sucrose	1491:1497	arg1	group					1499:1503	the sucrose group	1487:1503	the sucrose group	1487:1503	In the midgut, microbial diversity of the sucrose group was higher than that of the honey and high-fructose syrup groups, but in the hindgut, microbial diversity of the honey and high-fructose groups was higher than that in the sucrose group.
32183692	11	5	theme	relative	1960:1967	arg1	abundance					1969:1977	the relative abundance	1956:1977	the relative abundance of the dominant bacterial communities in honeybee guts, not the taxa, which could affect the health and safety of honeybee colonies during overwintering	1956:2130	CONCLUSION The type of sugar used as winter food affected the relative abundance of the dominant bacterial communities in honeybee guts, not the taxa, which could affect the health and safety of honeybee colonies during overwintering.
32183692	2	6	from	loss	311:314	arg1	winter					319:324	winter	319:324	winter	319:324	Although colony loss in winter is now the primary restriction in beekeeping, the effects of different sugars as winter food on the health of honeybee colonies are not well understood.
32183692	8	7	theme	high-fructose	1442:1454	arg1	groups					1456:1461	the honey and high-fructose groups	1428:1461	groups	1456:1461	In the midgut, microbial diversity of the sucrose group was higher than that of the honey and high-fructose syrup groups, but in the hindgut, microbial diversity of the honey and high-fructose groups was higher than that in the sucrose group.
32183692	4	8	theme	V3-V4	797:801	arg1	region					803:808	the V3-V4 region	793:808	the V3-V4 region of 16S rDNA	793:820	RESULTS The bacterial communities in honeybee midguts and hindguts before winter and after bees were fed honey, sucrose, and high-fructose syrup as winter-food were determined by targeting the V3-V4 region of 16S rDNA using the Illumina MiSeq platform.
32183692	4	9	theme	bacterial	616:624	arg1	communities					626:636	The bacterial communities	612:636	The bacterial communities in honeybee midguts and hindguts before winter and after bees were fed honey, sucrose, and high-fructose syrup as winter-food	612:762	RESULTS The bacterial communities in honeybee midguts and hindguts before winter and after bees were fed honey, sucrose, and high-fructose syrup as winter-food were determined by targeting the V3-V4 region of 16S rDNA using the Illumina MiSeq platform.
32183692	1	10	theme	bee	169:171	arg1	products					173:180	bee products	169:180	bee products	169:180	BACKGROUND The health of honeybee colonies is critical for bee products and agricultural production, and colony health is closely associated with the bacteria in the guts of honeybees.
32183692	9	11	theme	honeybee	1760:1767	arg1	hindgut					1769:1775	honeybee hindgut	1760:1775	honeybee hindgut	1760:1775	Sucrose increased the relative abundance of Actinobacteria (Bifidobacteriales Bifidobacteriaceae) and Alphaproteobacteria (Rhizobiales and Mitochondria) of honeybee midgut, and honey enriched the Bacteroidetes and Gammaproteobacteria (Pasteurellales) in honeybee hindgut.
32183692	3	12	theme	different	522:530	arg1	diets					538:542	different sugar diets	522:542	different sugar diets	522:542	Therefore, in this study, the influence of different sugar diets on honeybee gut bacteria during overwintering was examined.
32183692	1	13	from	bacteria	260:267	arg1	guts					276:279	the guts	272:279	the guts of honeybees	272:292	BACKGROUND The health of honeybee colonies is critical for bee products and agricultural production, and colony health is closely associated with the bacteria in the guts of honeybees.
32183692	7	14	theme	dominant	1218:1225	arg1	taxa					1227:1230	the dominant taxa	1214:1230	the dominant taxa	1214:1230	However, the relative abundance of the dominant taxa was affected by those factors.
32183692	11	15	theme	communities	2005:2015	arg1	abundance					1969:1977	the relative abundance	1956:1977	the relative abundance of the dominant bacterial communities in honeybee guts, not the taxa, which could affect the health and safety of honeybee colonies during overwintering	1956:2130	CONCLUSION The type of sugar used as winter food affected the relative abundance of the dominant bacterial communities in honeybee guts, not the taxa, which could affect the health and safety of honeybee colonies during overwintering.
32183692	3	16	theme	honeybee	547:554	arg1	bacteria					560:567	honeybee gut bacteria	547:567	honeybee gut bacteria	547:567	Therefore, in this study, the influence of different sugar diets on honeybee gut bacteria during overwintering was examined.
32183692	11	17	theme	winter	1935:1940	arg1	food					1942:1945	winter food	1935:1945	winter food	1935:1945	CONCLUSION The type of sugar used as winter food affected the relative abundance of the dominant bacterial communities in honeybee guts, not the taxa, which could affect the health and safety of honeybee colonies during overwintering.
32183692	8	18	theme	microbial	1278:1286	arg1	diversity					1288:1296	microbial diversity	1278:1296	microbial diversity of the sucrose group	1278:1317	In the midgut, microbial diversity of the sucrose group was higher than that of the honey and high-fructose syrup groups, but in the hindgut, microbial diversity of the honey and high-fructose groups was higher than that in the sucrose group.
32183692	10	19	theme	Betaproteobacteria	1834:1851	arg1	abundance					1821:1829	the relative abundance	1808:1829	the relative abundance of Betaproteobacteria (Neisseriales: Neisseriaceae) of the midgut	1808:1895	High-fructose syrup increased the relative abundance of Betaproteobacteria (Neisseriales: Neisseriaceae) of the midgut.
32183692	3	20	theme	diets	538:542	arg1	influence					509:517	the influence	505:517	the influence of different sugar diets on honeybee gut bacteria during overwintering	505:588	Therefore, in this study, the influence of different sugar diets on honeybee gut bacteria during overwintering was examined.
32183692	5	21	theme	dominant	861:868	arg1	Proteobacteria					913:926	the phyla Proteobacteria	903:926	the phyla Proteobacteria (63.17%)	903:935	The dominant microbiota in honeybee guts were the phyla Proteobacteria (63.17%), Firmicutes (17.61%; Lactobacillus, 15.91%), Actinobacteria (4.06%; Bifidobacterium, 3.34%), and Bacteroidetes (1.72%).
32183692	5	21	theme	dominant	861:868	arg1	microbiota					870:879	The dominant microbiota	857:879	The dominant microbiota in honeybee guts	857:896	The dominant microbiota in honeybee guts were the phyla Proteobacteria (63.17%), Firmicutes (17.61%; Lactobacillus, 15.91%), Actinobacteria (4.06%; Bifidobacterium, 3.34%), and Bacteroidetes (1.72%).
32183692	7	22	theme	relative	1192:1199	arg1	abundance					1201:1209	the relative abundance	1188:1209	the relative abundance of the dominant taxa	1188:1230	However, the relative abundance of the dominant taxa was affected by those factors.
32183692	3	23	from	influence	509:517	arg1	bacteria					560:567	honeybee gut bacteria	547:567	honeybee gut bacteria	547:567	Therefore, in this study, the influence of different sugar diets on honeybee gut bacteria during overwintering was examined.
32183692	9	24	theme	Actinobacteria	1550:1563	arg1	abundance					1537:1545	the relative abundance	1524:1545	the relative abundance of Actinobacteria (Bifidobacteriales Bifidobacteriaceae) and Alphaproteobacteria (Rhizobiales and Mitochondria) of honeybee midgut	1524:1676	Sucrose increased the relative abundance of Actinobacteria (Bifidobacteriales Bifidobacteriaceae) and Alphaproteobacteria (Rhizobiales and Mitochondria) of honeybee midgut, and honey enriched the Bacteroidetes and Gammaproteobacteria (Pasteurellales) in honeybee hindgut.
32183692	8	25	theme	sucrose	1305:1311	arg1	group					1313:1317	the sucrose group	1301:1317	the sucrose group	1301:1317	In the midgut, microbial diversity of the sucrose group was higher than that of the honey and high-fructose syrup groups, but in the hindgut, microbial diversity of the honey and high-fructose groups was higher than that in the sucrose group.
32183692	6	26	theme	dominant	1061:1068	arg1	taxa					1070:1073	The dominant taxa	1057:1073	The dominant taxa	1057:1073	The dominant taxa were conserved and not affected by season, type of overwintering sugar, or spatial position in the gut.
32183692	1	27	theme	colonies	144:151	arg1	health					125:130	The health	121:130	The health of honeybee colonies	121:151	BACKGROUND The health of honeybee colonies is critical for bee products and agricultural production, and colony health is closely associated with the bacteria in the guts of honeybees.
32183692	1	27	theme	colonies	144:151	arg1	critical					156:163	critical	156:163	critical	156:163	BACKGROUND The health of honeybee colonies is critical for bee products and agricultural production, and colony health is closely associated with the bacteria in the guts of honeybees.
32183692	0	28	theme	microbiota	65:74	arg1	composition					42:52	the composition	38:52	the composition of the gut microbiota in honeybee	38:86	The different dietary sugars modulate the composition of the gut microbiota in honeybee during overwintering.
32183692	12	29	theme	Alphaproteobacteria	2162:2180	arg1	presence					2137:2144	The presence	2133:2144	The presence of the supernal Alphaproteobacteria, Bifidobacteriales, and Lactobacillaceae in the gut of honeybees	2133:2245	The presence of the supernal Alphaproteobacteria, Bifidobacteriales, and Lactobacillaceae in the gut of honeybees fed sucrose and cheaper than honey both indicate that sucrose is very suitable as the overwintering food for honeybees.
32183692	11	30	dep	CONCLUSION	1898:1907	arg1	affected					1947:1954	affected	1947:1954	affected the relative abundance of the dominant bacterial communities in honeybee guts, not the taxa, which could affect the health and safety of honeybee colonies during overwintering	1947:2130	CONCLUSION The type of sugar used as winter food affected the relative abundance of the dominant bacterial communities in honeybee guts, not the taxa, which could affect the health and safety of honeybee colonies during overwintering.
32183692	9	31	theme	Alphaproteobacteria	1608:1626	arg1	abundance					1537:1545	the relative abundance	1524:1545	the relative abundance of Actinobacteria (Bifidobacteriales Bifidobacteriaceae) and Alphaproteobacteria (Rhizobiales and Mitochondria) of honeybee midgut	1524:1676	Sucrose increased the relative abundance of Actinobacteria (Bifidobacteriales Bifidobacteriaceae) and Alphaproteobacteria (Rhizobiales and Mitochondria) of honeybee midgut, and honey enriched the Bacteroidetes and Gammaproteobacteria (Pasteurellales) in honeybee hindgut.
32183692	0	32	from	composition	42:52	arg1	honeybee					79:86	honeybee	79:86	honeybee	79:86	The different dietary sugars modulate the composition of the gut microbiota in honeybee during overwintering.
32183692	1	33	dep	BACKGROUND	110:119	arg1	health					125:130	The health	121:130	The health of honeybee colonies	121:151	BACKGROUND The health of honeybee colonies is critical for bee products and agricultural production, and colony health is closely associated with the bacteria in the guts of honeybees.
32183692	1	33	dep	BACKGROUND	110:119	arg1	critical					156:163	critical	156:163	critical	156:163	BACKGROUND The health of honeybee colonies is critical for bee products and agricultural production, and colony health is closely associated with the bacteria in the guts of honeybees.
32183692	5	34	from	microbiota	870:879	arg1	guts					893:896	honeybee guts	884:896	honeybee guts	884:896	The dominant microbiota in honeybee guts were the phyla Proteobacteria (63.17%), Firmicutes (17.61%; Lactobacillus, 15.91%), Actinobacteria (4.06%; Bifidobacterium, 3.34%), and Bacteroidetes (1.72%).
32183692	11	35	theme	honeybee	2020:2027	arg1	guts					2029:2032	honeybee guts	2020:2032	honeybee guts	2020:2032	CONCLUSION The type of sugar used as winter food affected the relative abundance of the dominant bacterial communities in honeybee guts, not the taxa, which could affect the health and safety of honeybee colonies during overwintering.
32183692	11	35	theme	honeybee	2020:2027	arg1	taxa					2043:2046	the taxa	2039:2046	the taxa	2039:2046	CONCLUSION The type of sugar used as winter food affected the relative abundance of the dominant bacterial communities in honeybee guts, not the taxa, which could affect the health and safety of honeybee colonies during overwintering.
32183692	12	36	from	presence	2137:2144	arg1	gut					2230:2232	the gut	2226:2232	the gut of honeybees	2226:2245	The presence of the supernal Alphaproteobacteria, Bifidobacteriales, and Lactobacillaceae in the gut of honeybees fed sucrose and cheaper than honey both indicate that sucrose is very suitable as the overwintering food for honeybees.
32183692	9	37	dep	Bacteroidetes	1702:1714	arg1	the					1698:1700	the	1698:1700	the	1698:1700	Sucrose increased the relative abundance of Actinobacteria (Bifidobacteriales Bifidobacteriaceae) and Alphaproteobacteria (Rhizobiales and Mitochondria) of honeybee midgut, and honey enriched the Bacteroidetes and Gammaproteobacteria (Pasteurellales) in honeybee hindgut.
32183692	11	38	theme	dominant	1986:1993	arg1	communities					2005:2015	the dominant bacterial communities	1982:2015	the dominant bacterial communities	1982:2015	CONCLUSION The type of sugar used as winter food affected the relative abundance of the dominant bacterial communities in honeybee guts, not the taxa, which could affect the health and safety of honeybee colonies during overwintering.
32183692	2	39	theme	winter	407:412	arg1	food					414:417	winter food	407:417	winter food	407:417	Although colony loss in winter is now the primary restriction in beekeeping, the effects of different sugars as winter food on the health of honeybee colonies are not well understood.
32183692	8	40	theme	high-fructose	1357:1369	arg1	groups					1377:1382	the honey and high-fructose syrup groups	1343:1382	groups	1377:1382	In the midgut, microbial diversity of the sucrose group was higher than that of the honey and high-fructose syrup groups, but in the hindgut, microbial diversity of the honey and high-fructose groups was higher than that in the sucrose group.
32183692	2	41	theme	sugars	397:402	arg1	effects					376:382	the effects	372:382	the effects of different sugars as winter food on the health of honeybee colonies	372:452	Although colony loss in winter is now the primary restriction in beekeeping, the effects of different sugars as winter food on the health of honeybee colonies are not well understood.
32183692	0	42	theme	dietary	14:20	arg1	sugars					22:27	The different dietary sugars	0:27	The different dietary sugars	0:27	The different dietary sugars modulate the composition of the gut microbiota in honeybee during overwintering.
32183692	1	43	theme	honeybee	135:142	arg1	colonies					144:151	honeybee colonies	135:151	honeybee colonies	135:151	BACKGROUND The health of honeybee colonies is critical for bee products and agricultural production, and colony health is closely associated with the bacteria in the guts of honeybees.
32183692	2	44	theme	honeybee	436:443	arg1	colonies					445:452	honeybee colonies	436:452	honeybee colonies	436:452	Although colony loss in winter is now the primary restriction in beekeeping, the effects of different sugars as winter food on the health of honeybee colonies are not well understood.
32183692	5	45	dep	%	955:955	arg1	Lactobacillus					958:970	Lactobacillus	958:970	Lactobacillus	958:970	The dominant microbiota in honeybee guts were the phyla Proteobacteria (63.17%), Firmicutes (17.61%; Lactobacillus, 15.91%), Actinobacteria (4.06%; Bifidobacterium, 3.34%), and Bacteroidetes (1.72%).
32183692	1	46	theme	honeybees	284:292	arg1	guts					276:279	the guts	272:279	the guts of honeybees	272:292	BACKGROUND The health of honeybee colonies is critical for bee products and agricultural production, and colony health is closely associated with the bacteria in the guts of honeybees.
32183692	9	47	theme	midgut	1671:1676	arg1	abundance					1537:1545	the relative abundance	1524:1545	the relative abundance of Actinobacteria (Bifidobacteriales Bifidobacteriaceae) and Alphaproteobacteria (Rhizobiales and Mitochondria) of honeybee midgut	1524:1676	Sucrose increased the relative abundance of Actinobacteria (Bifidobacteriales Bifidobacteriaceae) and Alphaproteobacteria (Rhizobiales and Mitochondria) of honeybee midgut, and honey enriched the Bacteroidetes and Gammaproteobacteria (Pasteurellales) in honeybee hindgut.
32183692	8	48	theme	microbial	1405:1413	arg1	diversity					1415:1423	microbial diversity	1405:1423	microbial diversity of the honey and high-fructose groups	1405:1461	In the midgut, microbial diversity of the sucrose group was higher than that of the honey and high-fructose syrup groups, but in the hindgut, microbial diversity of the honey and high-fructose groups was higher than that in the sucrose group.
32183692	5	49	dep	%	1002:1002	arg1	Bifidobacterium					1005:1019	Bifidobacterium	1005:1019	Bifidobacterium	1005:1019	The dominant microbiota in honeybee guts were the phyla Proteobacteria (63.17%), Firmicutes (17.61%; Lactobacillus, 15.91%), Actinobacteria (4.06%; Bifidobacterium, 3.34%), and Bacteroidetes (1.72%).
32183692	8	50	theme	groups	1456:1461	arg1	diversity					1415:1423	microbial diversity	1405:1423	microbial diversity of the honey and high-fructose groups	1405:1461	In the midgut, microbial diversity of the sucrose group was higher than that of the honey and high-fructose syrup groups, but in the hindgut, microbial diversity of the honey and high-fructose groups was higher than that in the sucrose group.
32183692	1	51	theme	colony	215:220	arg1	health					222:227	colony health	215:227	colony health	215:227	BACKGROUND The health of honeybee colonies is critical for bee products and agricultural production, and colony health is closely associated with the bacteria in the guts of honeybees.
32183692	4	52	theme	honeybee	641:648	arg1	midguts					650:656	honeybee midguts	641:656	honeybee midguts	641:656	RESULTS The bacterial communities in honeybee midguts and hindguts before winter and after bees were fed honey, sucrose, and high-fructose syrup as winter-food were determined by targeting the V3-V4 region of 16S rDNA using the Illumina MiSeq platform.
32183692	6	53	from	season	1110:1115	arg1	gut					1174:1176	the gut	1170:1176	the gut	1170:1176	The dominant taxa were conserved and not affected by season, type of overwintering sugar, or spatial position in the gut.
32183692	4	54	theme	16S	813:815	arg1	rDNA					817:820	16S rDNA	813:820	16S rDNA	813:820	RESULTS The bacterial communities in honeybee midguts and hindguts before winter and after bees were fed honey, sucrose, and high-fructose syrup as winter-food were determined by targeting the V3-V4 region of 16S rDNA using the Illumina MiSeq platform.
32183692	12	55	theme	Lactobacillaceae	2206:2221	arg1	presence					2137:2144	The presence	2133:2144	The presence of the supernal Alphaproteobacteria, Bifidobacteriales, and Lactobacillaceae in the gut of honeybees	2133:2245	The presence of the supernal Alphaproteobacteria, Bifidobacteriales, and Lactobacillaceae in the gut of honeybees fed sucrose and cheaper than honey both indicate that sucrose is very suitable as the overwintering food for honeybees.
32183692	6	56	theme	overwintering	1126:1138	arg1	sugar					1140:1144	overwintering sugar	1126:1144	overwintering sugar	1126:1144	The dominant taxa were conserved and not affected by season, type of overwintering sugar, or spatial position in the gut.
32183692	6	57	from	type	1118:1121	arg1	gut					1174:1176	the gut	1170:1176	the gut	1170:1176	The dominant taxa were conserved and not affected by season, type of overwintering sugar, or spatial position in the gut.
32183692	11	58	theme	colonies	2102:2109	arg1	health					2072:2077	health	2072:2077	health	2072:2077	CONCLUSION The type of sugar used as winter food affected the relative abundance of the dominant bacterial communities in honeybee guts, not the taxa, which could affect the health and safety of honeybee colonies during overwintering.
32183692	11	58	theme	colonies	2102:2109	arg1	safety					2083:2088	safety	2083:2088	safety	2083:2088	CONCLUSION The type of sugar used as winter food affected the relative abundance of the dominant bacterial communities in honeybee guts, not the taxa, which could affect the health and safety of honeybee colonies during overwintering.
32183692	11	59	theme	honeybee	2093:2100	arg1	colonies					2102:2109	honeybee colonies	2093:2109	honeybee colonies	2093:2109	CONCLUSION The type of sugar used as winter food affected the relative abundance of the dominant bacterial communities in honeybee guts, not the taxa, which could affect the health and safety of honeybee colonies during overwintering.
32183692	10	60	theme	High-fructose	1778:1790	arg1	syrup					1792:1796	High-fructose syrup	1778:1796	High-fructose syrup	1778:1796	High-fructose syrup increased the relative abundance of Betaproteobacteria (Neisseriales: Neisseriaceae) of the midgut.
32183692	4	61	from	communities	626:636	arg1	hindguts					662:669	hindguts	662:669	hindguts	662:669	RESULTS The bacterial communities in honeybee midguts and hindguts before winter and after bees were fed honey, sucrose, and high-fructose syrup as winter-food were determined by targeting the V3-V4 region of 16S rDNA using the Illumina MiSeq platform.
32183692	4	61	from	communities	626:636	arg1	midguts					650:656	honeybee midguts	641:656	honeybee midguts	641:656	RESULTS The bacterial communities in honeybee midguts and hindguts before winter and after bees were fed honey, sucrose, and high-fructose syrup as winter-food were determined by targeting the V3-V4 region of 16S rDNA using the Illumina MiSeq platform.
32183692	11	62	theme	bacterial	1995:2003	arg1	communities					2005:2015	the dominant bacterial communities	1982:2015	the dominant bacterial communities	1982:2015	CONCLUSION The type of sugar used as winter food affected the relative abundance of the dominant bacterial communities in honeybee guts, not the taxa, which could affect the health and safety of honeybee colonies during overwintering.
32183692	2	63	theme	colony	304:309	arg1	loss					311:314	colony loss	304:314	colony loss in winter	304:324	Although colony loss in winter is now the primary restriction in beekeeping, the effects of different sugars as winter food on the health of honeybee colonies are not well understood.
32183692	2	63	theme	colony	304:309	arg1	restriction					345:355	the primary restriction	333:355	the primary restriction in beekeeping	333:369	Although colony loss in winter is now the primary restriction in beekeeping, the effects of different sugars as winter food on the health of honeybee colonies are not well understood.
32183692	2	64	from	restriction	345:355	arg1	beekeeping					360:369	beekeeping	360:369	beekeeping	360:369	Although colony loss in winter is now the primary restriction in beekeeping, the effects of different sugars as winter food on the health of honeybee colonies are not well understood.
32183692	10	65	theme	relative	1812:1819	arg1	abundance					1821:1829	the relative abundance	1808:1829	the relative abundance of Betaproteobacteria (Neisseriales: Neisseriaceae) of the midgut	1808:1895	High-fructose syrup increased the relative abundance of Betaproteobacteria (Neisseriales: Neisseriaceae) of the midgut.
32183692	1	66	theme	agricultural	186:197	arg1	production					199:208	agricultural production	186:208	agricultural production	186:208	BACKGROUND The health of honeybee colonies is critical for bee products and agricultural production, and colony health is closely associated with the bacteria in the guts of honeybees.
32183692	11	67	theme	sugar	1921:1925	arg1	type					1913:1916	The type	1909:1916	The type of sugar used as winter food	1909:1945	CONCLUSION The type of sugar used as winter food affected the relative abundance of the dominant bacterial communities in honeybee guts, not the taxa, which could affect the health and safety of honeybee colonies during overwintering.
32183692	12	68	theme	Bifidobacteriales	2183:2199	arg1	presence					2137:2144	The presence	2133:2144	The presence of the supernal Alphaproteobacteria, Bifidobacteriales, and Lactobacillaceae in the gut of honeybees	2133:2245	The presence of the supernal Alphaproteobacteria, Bifidobacteriales, and Lactobacillaceae in the gut of honeybees fed sucrose and cheaper than honey both indicate that sucrose is very suitable as the overwintering food for honeybees.
32183692	5	69	dep	Firmicutes	938:947	arg1	%					978:978	15.91%	973:978	15.91%	973:978	The dominant microbiota in honeybee guts were the phyla Proteobacteria (63.17%), Firmicutes (17.61%; Lactobacillus, 15.91%), Actinobacteria (4.06%; Bifidobacterium, 3.34%), and Bacteroidetes (1.72%).
32183692	5	69	dep	Firmicutes	938:947	arg1	%					955:955	17.61%	950:955	17.61%	950:955	The dominant microbiota in honeybee guts were the phyla Proteobacteria (63.17%), Firmicutes (17.61%; Lactobacillus, 15.91%), Actinobacteria (4.06%; Bifidobacterium, 3.34%), and Bacteroidetes (1.72%).
32183692	7	70	theme	taxa	1227:1230	arg1	abundance					1201:1209	the relative abundance	1188:1209	the relative abundance of the dominant taxa	1188:1230	However, the relative abundance of the dominant taxa was affected by those factors.
32183692	12	71	theme	honeybees	2237:2245	arg1	gut					2230:2232	the gut	2226:2232	the gut of honeybees	2226:2245	The presence of the supernal Alphaproteobacteria, Bifidobacteriales, and Lactobacillaceae in the gut of honeybees fed sucrose and cheaper than honey both indicate that sucrose is very suitable as the overwintering food for honeybees.
32183692	9	72	dep	Alphaproteobacteria	1608:1626	arg1	Mitochondria					1645:1656	Mitochondria	1645:1656	Mitochondria	1645:1656	Sucrose increased the relative abundance of Actinobacteria (Bifidobacteriales Bifidobacteriaceae) and Alphaproteobacteria (Rhizobiales and Mitochondria) of honeybee midgut, and honey enriched the Bacteroidetes and Gammaproteobacteria (Pasteurellales) in honeybee hindgut.
32183692	9	72	dep	Alphaproteobacteria	1608:1626	arg1	Rhizobiales					1629:1639	Rhizobiales	1629:1639	Rhizobiales	1629:1639	Sucrose increased the relative abundance of Actinobacteria (Bifidobacteriales Bifidobacteriaceae) and Alphaproteobacteria (Rhizobiales and Mitochondria) of honeybee midgut, and honey enriched the Bacteroidetes and Gammaproteobacteria (Pasteurellales) in honeybee hindgut.
32183692	3	73	theme	sugar	532:536	arg1	diets					538:542	different sugar diets	522:542	different sugar diets	522:542	Therefore, in this study, the influence of different sugar diets on honeybee gut bacteria during overwintering was examined.
32183692	9	74	theme	relative	1528:1535	arg1	abundance					1537:1545	the relative abundance	1524:1545	the relative abundance of Actinobacteria (Bifidobacteriales Bifidobacteriaceae) and Alphaproteobacteria (Rhizobiales and Mitochondria) of honeybee midgut	1524:1676	Sucrose increased the relative abundance of Actinobacteria (Bifidobacteriales Bifidobacteriaceae) and Alphaproteobacteria (Rhizobiales and Mitochondria) of honeybee midgut, and honey enriched the Bacteroidetes and Gammaproteobacteria (Pasteurellales) in honeybee hindgut.
32183692	11	75	from	abundance	1969:1977	arg1	guts					2029:2032	honeybee guts	2020:2032	honeybee guts	2020:2032	CONCLUSION The type of sugar used as winter food affected the relative abundance of the dominant bacterial communities in honeybee guts, not the taxa, which could affect the health and safety of honeybee colonies during overwintering.
32183692	11	75	from	abundance	1969:1977	arg1	taxa					2043:2046	the taxa	2039:2046	the taxa	2039:2046	CONCLUSION The type of sugar used as winter food affected the relative abundance of the dominant bacterial communities in honeybee guts, not the taxa, which could affect the health and safety of honeybee colonies during overwintering.
32183692	6	76	theme	spatial	1150:1156	arg1	position					1158:1165	spatial position	1150:1165	spatial position in the gut	1150:1176	The dominant taxa were conserved and not affected by season, type of overwintering sugar, or spatial position in the gut.
32183692	12	77	dep	fed	2247:2249	arg1	both					2282:2285	both	2282:2285	both	2282:2285	The presence of the supernal Alphaproteobacteria, Bifidobacteriales, and Lactobacillaceae in the gut of honeybees fed sucrose and cheaper than honey both indicate that sucrose is very suitable as the overwintering food for honeybees.
32183692	8	78	theme	group	1313:1317	arg1	diversity					1288:1296	microbial diversity	1278:1296	microbial diversity of the sucrose group	1278:1317	In the midgut, microbial diversity of the sucrose group was higher than that of the honey and high-fructose syrup groups, but in the hindgut, microbial diversity of the honey and high-fructose groups was higher than that in the sucrose group.
32183692	0	79	theme	gut	61:63	arg1	microbiota					65:74	the gut microbiota	57:74	the gut microbiota	57:74	The different dietary sugars modulate the composition of the gut microbiota in honeybee during overwintering.
32183692	3	80	theme	gut	556:558	arg1	bacteria					560:567	honeybee gut bacteria	547:567	honeybee gut bacteria	547:567	Therefore, in this study, the influence of different sugar diets on honeybee gut bacteria during overwintering was examined.
32183692	4	81	theme	Illumina	832:839	arg1	platform					847:854	the Illumina MiSeq platform	828:854	the Illumina MiSeq platform	828:854	RESULTS The bacterial communities in honeybee midguts and hindguts before winter and after bees were fed honey, sucrose, and high-fructose syrup as winter-food were determined by targeting the V3-V4 region of 16S rDNA using the Illumina MiSeq platform.
32183692	10	82	dep	Betaproteobacteria	1834:1851	arg1	Neisseriales					1854:1865	Neisseriales	1854:1865	Neisseriales	1854:1865	High-fructose syrup increased the relative abundance of Betaproteobacteria (Neisseriales: Neisseriaceae) of the midgut.
32183692	6	83	dep	conserved	1080:1088	arg1	affected					1098:1105	affected	1098:1105	affected by season, type of overwintering sugar, or spatial position in the gut	1098:1176	The dominant taxa were conserved and not affected by season, type of overwintering sugar, or spatial position in the gut.
32183692	2	84	theme	colonies	445:452	arg1	health					426:431	the health	422:431	the health of honeybee colonies	422:452	Although colony loss in winter is now the primary restriction in beekeeping, the effects of different sugars as winter food on the health of honeybee colonies are not well understood.
32183692	10	85	dep	Neisseriales	1854:1865	arg1	Neisseriaceae					1868:1880	Neisseriaceae	1868:1880	Neisseriaceae	1868:1880	High-fructose syrup increased the relative abundance of Betaproteobacteria (Neisseriales: Neisseriaceae) of the midgut.
32183692	12	86	attach	presence	2137:2144	arg2	Lactobacillaceae					2206:2221	Lactobacillaceae	2206:2221	Lactobacillaceae	2206:2221	The presence of the supernal Alphaproteobacteria, Bifidobacteriales, and Lactobacillaceae in the gut of honeybees fed sucrose and cheaper than honey both indicate that sucrose is very suitable as the overwintering food for honeybees.
32183692	12	86	attach	presence	2137:2144	arg2	Bifidobacteriales					2183:2199	Bifidobacteriales	2183:2199	Bifidobacteriales	2183:2199	The presence of the supernal Alphaproteobacteria, Bifidobacteriales, and Lactobacillaceae in the gut of honeybees fed sucrose and cheaper than honey both indicate that sucrose is very suitable as the overwintering food for honeybees.
32183692	12	86	attach	presence	2137:2144	arg1	gut					2230:2232	the gut	2226:2232	the gut of honeybees	2226:2245	The presence of the supernal Alphaproteobacteria, Bifidobacteriales, and Lactobacillaceae in the gut of honeybees fed sucrose and cheaper than honey both indicate that sucrose is very suitable as the overwintering food for honeybees.
32183692	12	86	attach	presence	2137:2144	arg2	Alphaproteobacteria					2162:2180	Alphaproteobacteria	2162:2180	Alphaproteobacteria	2162:2180	The presence of the supernal Alphaproteobacteria, Bifidobacteriales, and Lactobacillaceae in the gut of honeybees fed sucrose and cheaper than honey both indicate that sucrose is very suitable as the overwintering food for honeybees.
32183692	5	87	theme	phyla	907:911	arg1	Proteobacteria					913:926	the phyla Proteobacteria	903:926	the phyla Proteobacteria (63.17%)	903:935	The dominant microbiota in honeybee guts were the phyla Proteobacteria (63.17%), Firmicutes (17.61%; Lactobacillus, 15.91%), Actinobacteria (4.06%; Bifidobacterium, 3.34%), and Bacteroidetes (1.72%).
32183692	5	87	theme	phyla	907:911	arg1	%					934:934	63.17%	929:934	63.17%	929:934	The dominant microbiota in honeybee guts were the phyla Proteobacteria (63.17%), Firmicutes (17.61%; Lactobacillus, 15.91%), Actinobacteria (4.06%; Bifidobacterium, 3.34%), and Bacteroidetes (1.72%).
32183692	5	87	theme	phyla	907:911	arg1	microbiota					870:879	The dominant microbiota	857:879	The dominant microbiota in honeybee guts	857:896	The dominant microbiota in honeybee guts were the phyla Proteobacteria (63.17%), Firmicutes (17.61%; Lactobacillus, 15.91%), Actinobacteria (4.06%; Bifidobacterium, 3.34%), and Bacteroidetes (1.72%).
32183692	0	88	theme	different	4:12	arg1	sugars					22:27	The different dietary sugars	0:27	The different dietary sugars	0:27	The different dietary sugars modulate the composition of the gut microbiota in honeybee during overwintering.
32183692	5	89	theme	honeybee	884:891	arg1	guts					893:896	honeybee guts	884:896	honeybee guts	884:896	The dominant microbiota in honeybee guts were the phyla Proteobacteria (63.17%), Firmicutes (17.61%; Lactobacillus, 15.91%), Actinobacteria (4.06%; Bifidobacterium, 3.34%), and Bacteroidetes (1.72%).
32183692	8	90	theme	syrup	1371:1375	arg1	groups					1377:1382	the honey and high-fructose syrup groups	1343:1382	groups	1377:1382	In the midgut, microbial diversity of the sucrose group was higher than that of the honey and high-fructose syrup groups, but in the hindgut, microbial diversity of the honey and high-fructose groups was higher than that in the sucrose group.
32183692	2	91	theme	different	387:395	arg1	sugars					397:402	different sugars	387:402	different sugars as winter food	387:417	Although colony loss in winter is now the primary restriction in beekeeping, the effects of different sugars as winter food on the health of honeybee colonies are not well understood.
32183692	4	92	dep	RESULTS	604:610	arg1	determined					769:778	determined	769:778	were determined by targeting the V3-V4 region of 16S rDNA using the Illumina MiSeq platform	764:854	RESULTS The bacterial communities in honeybee midguts and hindguts before winter and after bees were fed honey, sucrose, and high-fructose syrup as winter-food were determined by targeting the V3-V4 region of 16S rDNA using the Illumina MiSeq platform.
32183692	9	93	theme	honeybee	1662:1669	arg1	midgut					1671:1676	honeybee midgut	1662:1676	honeybee midgut	1662:1676	Sucrose increased the relative abundance of Actinobacteria (Bifidobacteriales Bifidobacteriaceae) and Alphaproteobacteria (Rhizobiales and Mitochondria) of honeybee midgut, and honey enriched the Bacteroidetes and Gammaproteobacteria (Pasteurellales) in honeybee hindgut.
32183692	11	94	dep	health	2072:2077	arg1	the					2068:2070	the	2068:2070	the	2068:2070	CONCLUSION The type of sugar used as winter food affected the relative abundance of the dominant bacterial communities in honeybee guts, not the taxa, which could affect the health and safety of honeybee colonies during overwintering.
32183692	6	95	from	position	1158:1165	arg1	gut					1174:1176	the gut	1170:1176	the gut	1170:1176	The dominant taxa were conserved and not affected by season, type of overwintering sugar, or spatial position in the gut.
32183692	5	96	dep	Actinobacteria	982:995	arg1	%					1026:1026	3.34%	1022:1026	3.34%	1022:1026	The dominant microbiota in honeybee guts were the phyla Proteobacteria (63.17%), Firmicutes (17.61%; Lactobacillus, 15.91%), Actinobacteria (4.06%; Bifidobacterium, 3.34%), and Bacteroidetes (1.72%).
32183692	5	96	dep	Actinobacteria	982:995	arg1	%					1002:1002	4.06%	998:1002	4.06%	998:1002	The dominant microbiota in honeybee guts were the phyla Proteobacteria (63.17%), Firmicutes (17.61%; Lactobacillus, 15.91%), Actinobacteria (4.06%; Bifidobacterium, 3.34%), and Bacteroidetes (1.72%).
32183692	4	97	theme	high-fructose	729:741	arg1	syrup					743:747	high-fructose syrup	729:747	high-fructose syrup	729:747	RESULTS The bacterial communities in honeybee midguts and hindguts before winter and after bees were fed honey, sucrose, and high-fructose syrup as winter-food were determined by targeting the V3-V4 region of 16S rDNA using the Illumina MiSeq platform.
32183692	12	98	theme	overwintering	2333:2345	arg1	food					2347:2350	the overwintering food	2329:2350	the overwintering food for honeybees	2329:2364	The presence of the supernal Alphaproteobacteria, Bifidobacteriales, and Lactobacillaceae in the gut of honeybees fed sucrose and cheaper than honey both indicate that sucrose is very suitable as the overwintering food for honeybees.
32183692	2	99	from	effects	376:382	arg1	health					426:431	the health	422:431	the health of honeybee colonies	422:452	Although colony loss in winter is now the primary restriction in beekeeping, the effects of different sugars as winter food on the health of honeybee colonies are not well understood.
32183692	2	100	theme	primary	337:343	arg1	loss					311:314	colony loss	304:314	colony loss in winter	304:324	Although colony loss in winter is now the primary restriction in beekeeping, the effects of different sugars as winter food on the health of honeybee colonies are not well understood.
32183692	2	100	theme	primary	337:343	arg1	restriction					345:355	the primary restriction	333:355	the primary restriction in beekeeping	333:369	Although colony loss in winter is now the primary restriction in beekeeping, the effects of different sugars as winter food on the health of honeybee colonies are not well understood.
33177483	5	0	with	group	657:661	arg1	N=6					683:685	N=6	683:685	N=6	683:685	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	5	0	with	group	657:661	arg1	N=6					643:645	N=6	643:645	N=6	643:645	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	5	0	with	group	657:661	arg1	diet					717:720	a normal diet	708:720	a normal diet	708:720	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	5	0	with	group	657:661	arg1	diet					677:680	a normal diet	668:680	a normal diet (N=6)	668:686	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	5	0	with	group	657:661	arg1	diet					784:787	a normal diet	775:787	a normal diet	775:787	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	5	0	with	group	657:661	arg1	diet					637:640	a normal diet	628:640	a normal diet (N=6)	628:646	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	5	1	theme	male	570:573	arg1	mice					582:585	Twenty-four male C57BL6 mice	558:585	Twenty-four male C57BL6 mice	558:585	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	10	2	theme	colonic	1452:1458	arg1	activity					1468:1475	colonic disease activity	1452:1475	colonic disease activity	1452:1475	In the DSS+rifaximin+vitamin D3 group, the therapeutic influences of rifaximin, in terms of weight loss and colonic disease activity, were significantly reduced, and the gut microbiota of the mice were completely changed in composition and diversity.
33177483	2	3	theme	due	235:237	arg1	IBD					231:233	IBD	231:233	IBD due to its effects on inflammatory cytokines	231:278	Vitamin D3 can control IBD due to its effects on inflammatory cytokines.
33177483	7	4	link	enzyme-linked	983:995	arg1	ELISA					1018:1022	ELISA	1018:1022	ELISA	1018:1022	Serum levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1ß (IL-1ß) and enzyme-linked immunosorbent assay (ELISA) were used to measure the level of IL-6 and P65, and phospho-p65 was measured by western blot.
33177483	7	4	link	enzyme-linked	983:995	arg1	assay					1011:1015	enzyme-linked immunosorbent assay	983:1015	enzyme-linked immunosorbent assay (ELISA)	983:1023	Serum levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1ß (IL-1ß) and enzyme-linked immunosorbent assay (ELISA) were used to measure the level of IL-6 and P65, and phospho-p65 was measured by western blot.
33177483	10	5	theme	activity	1468:1475	arg1	terms					1427:1431	terms	1427:1431	terms of weight loss and colonic disease activity	1427:1475	In the DSS+rifaximin+vitamin D3 group, the therapeutic influences of rifaximin, in terms of weight loss and colonic disease activity, were significantly reduced, and the gut microbiota of the mice were completely changed in composition and diversity.
33177483	1	6	theme	bowel	187:191	arg1	IBD					202:204	IBD	202:204	IBD	202:204	BACKGROUND Rifaximin is an antimicrobial agent used to treat inflammatory bowel disease (IBD).
33177483	1	6	theme	bowel	187:191	arg1	disease					193:199	inflammatory bowel disease	174:199	inflammatory bowel disease (IBD)	174:205	BACKGROUND Rifaximin is an antimicrobial agent used to treat inflammatory bowel disease (IBD).
33177483	4	7	theme	drinking	542:549	arg1	water					551:555	the drinking water	538:555	the drinking water	538:555	MATERIAL AND METHODS The mouse model of IBD was developed using DSS (4%) administered via the drinking water.
33177483	11	8	theme	vitamin	1647:1653	arg1	D3					1655:1656	vitamin D3	1647:1656	vitamin D3	1647:1656	CONCLUSIONS In a mouse model of IBD, treatment with vitamin D3 significantly increased the metabolism of rifaximin and reduced its therapeutic effects.
33177483	10	9	theme	disease	1460:1466	arg1	activity					1468:1475	colonic disease activity	1452:1475	colonic disease activity	1452:1475	In the DSS+rifaximin+vitamin D3 group, the therapeutic influences of rifaximin, in terms of weight loss and colonic disease activity, were significantly reduced, and the gut microbiota of the mice were completely changed in composition and diversity.
33177483	1	10	theme	BACKGROUND	113:122	arg1	agent					154:158	an antimicrobial agent	137:158	an antimicrobial agent used to treat inflammatory bowel disease (IBD)	137:205	BACKGROUND Rifaximin is an antimicrobial agent used to treat inflammatory bowel disease (IBD).
33177483	1	10	theme	BACKGROUND	113:122	arg1	Rifaximin					124:132	BACKGROUND Rifaximin	113:132	BACKGROUND Rifaximin	113:132	BACKGROUND Rifaximin is an antimicrobial agent used to treat inflammatory bowel disease (IBD).
33177483	2	11	from	effects	246:252	arg1	cytokines					270:278	inflammatory cytokines	257:278	inflammatory cytokines	257:278	Vitamin D3 can control IBD due to its effects on inflammatory cytokines.
33177483	11	12	from	CONCLUSIONS	1595:1605	arg1	model					1618:1622	a mouse model	1610:1622	a mouse model of IBD	1610:1629	CONCLUSIONS In a mouse model of IBD, treatment with vitamin D3 significantly increased the metabolism of rifaximin and reduced its therapeutic effects.
33177483	11	12	from	CONCLUSIONS	1595:1605	arg1	treatment					1632:1640	treatment	1632:1640	treatment with vitamin D3	1632:1656	CONCLUSIONS In a mouse model of IBD, treatment with vitamin D3 significantly increased the metabolism of rifaximin and reduced its therapeutic effects.
33177483	10	13	theme	gut	1514:1516	arg1	microbiota					1518:1527	the gut microbiota	1510:1527	the gut microbiota of the mice	1510:1539	In the DSS+rifaximin+vitamin D3 group, the therapeutic influences of rifaximin, in terms of weight loss and colonic disease activity, were significantly reduced, and the gut microbiota of the mice were completely changed in composition and diversity.
33177483	9	14	theme	inflammation	1240:1251	arg1	severity					1253:1260	the inflammation severity	1236:1260	the inflammation severity of the colon	1236:1273	RESULTS In the DSS mouse model of IBD, rifaximin reduced the inflammation severity of the colon and reduced the expression of phospho-p65, p65, TNF-alpha, and IL-6.
33177483	7	15	theme	enzyme-linked	983:995	arg1	ELISA					1018:1022	ELISA	1018:1022	ELISA	1018:1022	Serum levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1ß (IL-1ß) and enzyme-linked immunosorbent assay (ELISA) were used to measure the level of IL-6 and P65, and phospho-p65 was measured by western blot.
33177483	7	15	theme	enzyme-linked	983:995	arg1	assay					1011:1015	enzyme-linked immunosorbent assay	983:1015	enzyme-linked immunosorbent assay (ELISA)	983:1023	Serum levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1ß (IL-1ß) and enzyme-linked immunosorbent assay (ELISA) were used to measure the level of IL-6 and P65, and phospho-p65 was measured by western blot.
33177483	1	16	theme	antimicrobial	140:152	arg1	agent					154:158	an antimicrobial agent	137:158	an antimicrobial agent used to treat inflammatory bowel disease (IBD)	137:205	BACKGROUND Rifaximin is an antimicrobial agent used to treat inflammatory bowel disease (IBD).
33177483	1	16	theme	antimicrobial	140:152	arg1	Rifaximin					124:132	BACKGROUND Rifaximin	113:132	BACKGROUND Rifaximin	113:132	BACKGROUND Rifaximin is an antimicrobial agent used to treat inflammatory bowel disease (IBD).
33177483	0	17	from	Effects	0:6	arg1	Flora					36:40	Intestinal Flora	25:40	Intestinal Flora in a Mouse Model of Inflammatory Bowel Disease	25:87	Effects of Vitamin D3 on Intestinal Flora in a Mouse Model of Inflammatory Bowel Disease Treated with Rifaximin.
33177483	5	18	theme	DSS	760:762	arg1	group					764:768	the DSS group	756:768	the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6)	756:831	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	0	19	theme	Inflammatory	62:73	arg1	Disease					81:87	Inflammatory Bowel Disease	62:87	Inflammatory Bowel Disease	62:87	Effects of Vitamin D3 on Intestinal Flora in a Mouse Model of Inflammatory Bowel Disease Treated with Rifaximin.
33177483	9	20	theme	colon	1269:1273	arg1	severity					1253:1260	the inflammation severity	1236:1260	the inflammation severity of the colon	1236:1273	RESULTS In the DSS mouse model of IBD, rifaximin reduced the inflammation severity of the colon and reduced the expression of phospho-p65, p65, TNF-alpha, and IL-6.
33177483	3	21	theme	study	301:305	arg1	purpose					285:291	The purpose	281:291	The purpose of this study	281:305	The purpose of this study was to assess the effect of vitamin D3 on the intestinal flora of a dextran sulfate sodium (DSS)-induced mouse model treated with rifaximin.
33177483	0	22	from	Flora	36:40	arg1	Model					53:57	a Mouse Model	45:57	a Mouse Model of Inflammatory Bowel Disease	45:87	Effects of Vitamin D3 on Intestinal Flora in a Mouse Model of Inflammatory Bowel Disease Treated with Rifaximin.
33177483	10	23	theme	D3	1373:1374	arg1	group					1376:1380	the DSS+rifaximin+vitamin D3 group	1347:1380	the DSS+rifaximin+vitamin D3 group	1347:1380	In the DSS+rifaximin+vitamin D3 group, the therapeutic influences of rifaximin, in terms of weight loss and colonic disease activity, were significantly reduced, and the gut microbiota of the mice were completely changed in composition and diversity.
33177483	0	24	theme	Disease	81:87	arg1	Model					53:57	a Mouse Model	45:57	a Mouse Model of Inflammatory Bowel Disease	45:87	Effects of Vitamin D3 on Intestinal Flora in a Mouse Model of Inflammatory Bowel Disease Treated with Rifaximin.
33177483	0	25	theme	Intestinal	25:34	arg1	Flora					36:40	Intestinal Flora	25:40	Intestinal Flora in a Mouse Model of Inflammatory Bowel Disease	25:87	Effects of Vitamin D3 on Intestinal Flora in a Mouse Model of Inflammatory Bowel Disease Treated with Rifaximin.
33177483	5	26	theme	normal	777:782	arg1	diet					784:787	a normal diet	775:787	a normal diet	775:787	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	0	27	theme	Mouse	47:51	arg1	Model					53:57	a Mouse Model	45:57	a Mouse Model of Inflammatory Bowel Disease	45:87	Effects of Vitamin D3 on Intestinal Flora in a Mouse Model of Inflammatory Bowel Disease Treated with Rifaximin.
33177483	5	28	theme	normal	670:675	arg1	N=6					683:685	N=6	683:685	N=6	683:685	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	5	28	theme	normal	670:675	arg1	diet					677:680	a normal diet	668:680	a normal diet (N=6)	668:686	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	4	29	theme	mouse	473:477	arg1	model					479:483	The mouse model	469:483	METHODS The mouse model of IBD	461:490	MATERIAL AND METHODS The mouse model of IBD was developed using DSS (4%) administered via the drinking water.
33177483	8	30	theme	rRNA	1123:1126	arg1	sequencing					1133:1142	16S rRNA gene sequencing	1119:1142	16S rRNA gene sequencing	1119:1142	16S rRNA gene sequencing was used to analyze fecal samples.
33177483	10	31	theme	therapeutic	1387:1397	arg1	influences					1399:1408	the therapeutic influences	1383:1408	the therapeutic influences of rifaximin	1383:1421	In the DSS+rifaximin+vitamin D3 group, the therapeutic influences of rifaximin, in terms of weight loss and colonic disease activity, were significantly reduced, and the gut microbiota of the mice were completely changed in composition and diversity.
33177483	0	32	theme	Vitamin	11:17	arg1	D3					19:20	Vitamin D3	11:20	Vitamin D3	11:20	Effects of Vitamin D3 on Intestinal Flora in a Mouse Model of Inflammatory Bowel Disease Treated with Rifaximin.
33177483	9	33	theme	phospho-p65	1305:1315	arg1	expression					1291:1300	the expression	1287:1300	the expression of phospho-p65, p65, TNF-alpha, and IL-6	1287:1341	RESULTS In the DSS mouse model of IBD, rifaximin reduced the inflammation severity of the colon and reduced the expression of phospho-p65, p65, TNF-alpha, and IL-6.
33177483	5	34	with	group	617:621	arg1	N=6					683:685	N=6	683:685	N=6	683:685	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	5	34	with	group	617:621	arg1	N=6					643:645	N=6	643:645	N=6	643:645	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	5	34	with	group	617:621	arg1	diet					717:720	a normal diet	708:720	a normal diet	708:720	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	5	34	with	group	617:621	arg1	diet					677:680	a normal diet	668:680	a normal diet (N=6)	668:686	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	5	34	with	group	617:621	arg1	diet					784:787	a normal diet	775:787	a normal diet	775:787	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	5	34	with	group	617:621	arg1	diet					637:640	a normal diet	628:640	a normal diet (N=6)	628:646	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	11	35	theme	mouse	1612:1616	arg1	model					1618:1622	a mouse model	1610:1622	a mouse model of IBD	1610:1629	CONCLUSIONS In a mouse model of IBD, treatment with vitamin D3 significantly increased the metabolism of rifaximin and reduced its therapeutic effects.
33177483	11	35	theme	mouse	1612:1616	arg1	treatment					1632:1640	treatment	1632:1640	treatment with vitamin D3	1632:1656	CONCLUSIONS In a mouse model of IBD, treatment with vitamin D3 significantly increased the metabolism of rifaximin and reduced its therapeutic effects.
33177483	7	36	theme	factor-alpha	927:938	arg1	assay					1011:1015	enzyme-linked immunosorbent assay	983:1015	enzyme-linked immunosorbent assay (ELISA)	983:1023	Serum levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1ß (IL-1ß) and enzyme-linked immunosorbent assay (ELISA) were used to measure the level of IL-6 and P65, and phospho-p65 was measured by western blot.
33177483	7	36	theme	factor-alpha	927:938	arg1	ELISA					1018:1022	ELISA	1018:1022	ELISA	1018:1022	Serum levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1ß (IL-1ß) and enzyme-linked immunosorbent assay (ELISA) were used to measure the level of IL-6 and P65, and phospho-p65 was measured by western blot.
33177483	7	36	theme	factor-alpha	927:938	arg1	levels					902:907	Serum levels	896:907	Serum levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1ß (IL-1ß)	896:977	Serum levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1ß (IL-1ß) and enzyme-linked immunosorbent assay (ELISA) were used to measure the level of IL-6 and P65, and phospho-p65 was measured by western blot.
33177483	5	37	theme	DSS	653:655	arg1	group					657:661	the DSS group	649:661	the DSS group with a normal diet (N=6)	649:686	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	9	38	theme	p65	1318:1320	arg1	expression					1291:1300	the expression	1287:1300	the expression of phospho-p65, p65, TNF-alpha, and IL-6	1287:1341	RESULTS In the DSS mouse model of IBD, rifaximin reduced the inflammation severity of the colon and reduced the expression of phospho-p65, p65, TNF-alpha, and IL-6.
33177483	3	39	theme	D3	343:344	arg1	effect					325:330	the effect	321:330	the effect of vitamin D3 on the intestinal flora of a dextran sulfate sodium (DSS)-induced mouse model treated with rifaximin	321:445	The purpose of this study was to assess the effect of vitamin D3 on the intestinal flora of a dextran sulfate sodium (DSS)-induced mouse model treated with rifaximin.
33177483	10	40	theme	mice	1536:1539	arg1	microbiota					1518:1527	the gut microbiota	1510:1527	the gut microbiota of the mice	1510:1539	In the DSS+rifaximin+vitamin D3 group, the therapeutic influences of rifaximin, in terms of weight loss and colonic disease activity, were significantly reduced, and the gut microbiota of the mice were completely changed in composition and diversity.
33177483	3	41	theme	mouse	412:416	arg1	model					418:422	a dextran sulfate sodium (DSS)-induced mouse model	373:422	a dextran sulfate sodium (DSS)-induced mouse model treated with rifaximin	373:445	The purpose of this study was to assess the effect of vitamin D3 on the intestinal flora of a dextran sulfate sodium (DSS)-induced mouse model treated with rifaximin.
33177483	7	42	theme	tumor	912:916	arg1	TNF-alpha					941:949	TNF-alpha	941:949	TNF-alpha	941:949	Serum levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1ß (IL-1ß) and enzyme-linked immunosorbent assay (ELISA) were used to measure the level of IL-6 and P65, and phospho-p65 was measured by western blot.
33177483	7	42	theme	tumor	912:916	arg1	factor-alpha					927:938	tumor necrosis factor-alpha	912:938	tumor necrosis factor-alpha (TNF-alpha)	912:950	Serum levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1ß (IL-1ß) and enzyme-linked immunosorbent assay (ELISA) were used to measure the level of IL-6 and P65, and phospho-p65 was measured by western blot.
33177483	9	43	theme	TNF-alpha	1323:1331	arg1	expression					1291:1300	the expression	1287:1300	the expression of phospho-p65, p65, TNF-alpha, and IL-6	1287:1341	RESULTS In the DSS mouse model of IBD, rifaximin reduced the inflammation severity of the colon and reduced the expression of phospho-p65, p65, TNF-alpha, and IL-6.
33177483	5	44	theme	vitamin	816:822	arg1	N=6					828:830	N=6	828:830	N=6	828:830	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	5	44	theme	vitamin	816:822	arg1	D3					824:825	vitamin D3	816:825	vitamin D3 (N=6)	816:831	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	10	45	theme	loss	1443:1446	arg1	terms					1427:1431	terms	1427:1431	terms of weight loss and colonic disease activity	1427:1475	In the DSS+rifaximin+vitamin D3 group, the therapeutic influences of rifaximin, in terms of weight loss and colonic disease activity, were significantly reduced, and the gut microbiota of the mice were completely changed in composition and diversity.
33177483	10	46	theme	rifaximin	1413:1421	arg1	influences					1399:1408	the therapeutic influences	1383:1408	the therapeutic influences of rifaximin	1383:1421	In the DSS+rifaximin+vitamin D3 group, the therapeutic influences of rifaximin, in terms of weight loss and colonic disease activity, were significantly reduced, and the gut microbiota of the mice were completely changed in composition and diversity.
33177483	10	47	theme	weight	1436:1441	arg1	loss					1443:1446	weight loss	1436:1446	weight loss	1436:1446	In the DSS+rifaximin+vitamin D3 group, the therapeutic influences of rifaximin, in terms of weight loss and colonic disease activity, were significantly reduced, and the gut microbiota of the mice were completely changed in composition and diversity.
33177483	7	48	theme	interleukin-1ß	956:969	arg1	assay					1011:1015	enzyme-linked immunosorbent assay	983:1015	enzyme-linked immunosorbent assay (ELISA)	983:1023	Serum levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1ß (IL-1ß) and enzyme-linked immunosorbent assay (ELISA) were used to measure the level of IL-6 and P65, and phospho-p65 was measured by western blot.
33177483	7	48	theme	interleukin-1ß	956:969	arg1	ELISA					1018:1022	ELISA	1018:1022	ELISA	1018:1022	Serum levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1ß (IL-1ß) and enzyme-linked immunosorbent assay (ELISA) were used to measure the level of IL-6 and P65, and phospho-p65 was measured by western blot.
33177483	7	48	theme	interleukin-1ß	956:969	arg1	levels					902:907	Serum levels	896:907	Serum levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1ß (IL-1ß)	896:977	Serum levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1ß (IL-1ß) and enzyme-linked immunosorbent assay (ELISA) were used to measure the level of IL-6 and P65, and phospho-p65 was measured by western blot.
33177483	7	49	theme	Serum	896:900	arg1	levels					902:907	Serum levels	896:907	Serum levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1ß (IL-1ß)	896:977	Serum levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1ß (IL-1ß) and enzyme-linked immunosorbent assay (ELISA) were used to measure the level of IL-6 and P65, and phospho-p65 was measured by western blot.
33177483	6	50	theme	colonic	853:859	arg1	tissue					861:866	the colonic tissue	849:866	the colonic tissue	849:866	After 14 days, the colonic tissue was studied histologically.
33177483	11	51	theme	rifaximin	1700:1708	arg1	metabolism					1686:1695	the metabolism	1682:1695	the metabolism of rifaximin	1682:1708	CONCLUSIONS In a mouse model of IBD, treatment with vitamin D3 significantly increased the metabolism of rifaximin and reduced its therapeutic effects.
33177483	5	52	theme	DSS	693:695	arg1	group					697:701	the DSS group	689:701	the DSS group with a normal diet treated with rifaximin (N=6)	689:749	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	11	53	theme	therapeutic	1726:1736	arg1	effects					1738:1744	its therapeutic effects	1722:1744	its therapeutic effects	1722:1744	CONCLUSIONS In a mouse model of IBD, treatment with vitamin D3 significantly increased the metabolism of rifaximin and reduced its therapeutic effects.
33177483	2	54	theme	inflammatory	257:268	arg1	cytokines					270:278	inflammatory cytokines	257:278	inflammatory cytokines	257:278	Vitamin D3 can control IBD due to its effects on inflammatory cytokines.
33177483	9	55	theme	DSS	1194:1196	arg1	model					1204:1208	the DSS mouse model	1190:1208	the DSS mouse model of IBD	1190:1215	RESULTS In the DSS mouse model of IBD, rifaximin reduced the inflammation severity of the colon and reduced the expression of phospho-p65, p65, TNF-alpha, and IL-6.
33177483	3	56	theme	model	418:422	arg1	flora					364:368	the intestinal flora	349:368	the intestinal flora of a dextran sulfate sodium (DSS)-induced mouse model treated with rifaximin	349:445	The purpose of this study was to assess the effect of vitamin D3 on the intestinal flora of a dextran sulfate sodium (DSS)-induced mouse model treated with rifaximin.
33177483	5	57	theme	control	609:615	arg1	group					617:621	the control group	605:621	the control group with a normal diet (N=6)	605:646	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	2	58	theme	Vitamin	208:214	arg1	D3					216:217	Vitamin D3	208:217	Vitamin D3	208:217	Vitamin D3 can control IBD due to its effects on inflammatory cytokines.
33177483	5	59	with	group	764:768	arg1	N=6					683:685	N=6	683:685	N=6	683:685	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	5	59	with	group	764:768	arg1	N=6					643:645	N=6	643:645	N=6	643:645	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	5	59	with	group	764:768	arg1	diet					717:720	a normal diet	708:720	a normal diet	708:720	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	5	59	with	group	764:768	arg1	diet					677:680	a normal diet	668:680	a normal diet (N=6)	668:686	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	5	59	with	group	764:768	arg1	diet					784:787	a normal diet	775:787	a normal diet	775:787	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	5	59	with	group	764:768	arg1	diet					637:640	a normal diet	628:640	a normal diet (N=6)	628:646	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	5	60	theme	C57BL6	575:580	arg1	mice					582:585	Twenty-four male C57BL6 mice	558:585	Twenty-four male C57BL6 mice	558:585	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	9	61	theme	IBD	1213:1215	arg1	model					1204:1208	the DSS mouse model	1190:1208	the DSS mouse model of IBD	1190:1215	RESULTS In the DSS mouse model of IBD, rifaximin reduced the inflammation severity of the colon and reduced the expression of phospho-p65, p65, TNF-alpha, and IL-6.
33177483	7	62	theme	western	1105:1111	arg1	blot					1113:1116	western blot	1105:1116	western blot	1105:1116	Serum levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1ß (IL-1ß) and enzyme-linked immunosorbent assay (ELISA) were used to measure the level of IL-6 and P65, and phospho-p65 was measured by western blot.
33177483	7	63	theme	immunosorbent	997:1009	arg1	ELISA					1018:1022	ELISA	1018:1022	ELISA	1018:1022	Serum levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1ß (IL-1ß) and enzyme-linked immunosorbent assay (ELISA) were used to measure the level of IL-6 and P65, and phospho-p65 was measured by western blot.
33177483	7	63	theme	immunosorbent	997:1009	arg1	assay					1011:1015	enzyme-linked immunosorbent assay	983:1015	enzyme-linked immunosorbent assay (ELISA)	983:1023	Serum levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1ß (IL-1ß) and enzyme-linked immunosorbent assay (ELISA) were used to measure the level of IL-6 and P65, and phospho-p65 was measured by western blot.
33177483	1	64	theme	inflammatory	174:185	arg1	IBD					202:204	IBD	202:204	IBD	202:204	BACKGROUND Rifaximin is an antimicrobial agent used to treat inflammatory bowel disease (IBD).
33177483	1	64	theme	inflammatory	174:185	arg1	disease					193:199	inflammatory bowel disease	174:199	inflammatory bowel disease (IBD)	174:205	BACKGROUND Rifaximin is an antimicrobial agent used to treat inflammatory bowel disease (IBD).
33177483	5	65	theme	normal	710:715	arg1	diet					717:720	a normal diet	708:720	a normal diet	708:720	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	11	66	with	treatment	1632:1640	arg1	D3					1655:1656	vitamin D3	1647:1656	vitamin D3	1647:1656	CONCLUSIONS In a mouse model of IBD, treatment with vitamin D3 significantly increased the metabolism of rifaximin and reduced its therapeutic effects.
33177483	4	67	dep	METHODS	461:467	arg1	model					479:483	The mouse model	469:483	METHODS The mouse model of IBD	461:490	MATERIAL AND METHODS The mouse model of IBD was developed using DSS (4%) administered via the drinking water.
33177483	7	68	used	used	1030:1033	arg2	levels					902:907	Serum levels	896:907	Serum levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1ß (IL-1ß)	896:977	Serum levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1ß (IL-1ß) and enzyme-linked immunosorbent assay (ELISA) were used to measure the level of IL-6 and P65, and phospho-p65 was measured by western blot.
33177483	7	68	used	used	1030:1033	arg2	assay					1011:1015	enzyme-linked immunosorbent assay	983:1015	enzyme-linked immunosorbent assay (ELISA)	983:1023	Serum levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1ß (IL-1ß) and enzyme-linked immunosorbent assay (ELISA) were used to measure the level of IL-6 and P65, and phospho-p65 was measured by western blot.
33177483	7	68	used	used	1030:1033	arg2	ELISA					1018:1022	ELISA	1018:1022	ELISA	1018:1022	Serum levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1ß (IL-1ß) and enzyme-linked immunosorbent assay (ELISA) were used to measure the level of IL-6 and P65, and phospho-p65 was measured by western blot.
33177483	0	69	theme	Bowel	75:79	arg1	Disease					81:87	Inflammatory Bowel Disease	62:87	Inflammatory Bowel Disease	62:87	Effects of Vitamin D3 on Intestinal Flora in a Mouse Model of Inflammatory Bowel Disease Treated with Rifaximin.
33177483	5	70	theme	normal	630:635	arg1	N=6					643:645	N=6	643:645	N=6	643:645	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	5	70	theme	normal	630:635	arg1	diet					637:640	a normal diet	628:640	a normal diet (N=6)	628:646	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	3	71	from	effect	325:330	arg1	flora					364:368	the intestinal flora	349:368	the intestinal flora of a dextran sulfate sodium (DSS)-induced mouse model treated with rifaximin	349:445	The purpose of this study was to assess the effect of vitamin D3 on the intestinal flora of a dextran sulfate sodium (DSS)-induced mouse model treated with rifaximin.
33177483	9	72	theme	mouse	1198:1202	arg1	model					1204:1208	the DSS mouse model	1190:1208	the DSS mouse model of IBD	1190:1215	RESULTS In the DSS mouse model of IBD, rifaximin reduced the inflammation severity of the colon and reduced the expression of phospho-p65, p65, TNF-alpha, and IL-6.
33177483	10	73	theme	DSS+rifaximin+vitamin	1351:1371	arg1	group					1376:1380	the DSS+rifaximin+vitamin D3 group	1347:1380	the DSS+rifaximin+vitamin D3 group	1347:1380	In the DSS+rifaximin+vitamin D3 group, the therapeutic influences of rifaximin, in terms of weight loss and colonic disease activity, were significantly reduced, and the gut microbiota of the mice were completely changed in composition and diversity.
33177483	7	74	theme	P65	1068:1070	arg1	level					1050:1054	the level	1046:1054	the level of IL-6 and P65	1046:1070	Serum levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1ß (IL-1ß) and enzyme-linked immunosorbent assay (ELISA) were used to measure the level of IL-6 and P65, and phospho-p65 was measured by western blot.
33177483	3	75	theme	vitamin	335:341	arg1	D3					343:344	vitamin D3	335:344	vitamin D3	335:344	The purpose of this study was to assess the effect of vitamin D3 on the intestinal flora of a dextran sulfate sodium (DSS)-induced mouse model treated with rifaximin.
33177483	0	76	theme	D3	19:20	arg1	Effects					0:6	Effects	0:6	Effects of Vitamin D3 on Intestinal Flora in a Mouse Model of Inflammatory Bowel Disease	0:87	Effects of Vitamin D3 on Intestinal Flora in a Mouse Model of Inflammatory Bowel Disease Treated with Rifaximin.
33177483	7	77	theme	IL-6	1059:1062	arg1	level					1050:1054	the level	1046:1054	the level of IL-6 and P65	1046:1070	Serum levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1ß (IL-1ß) and enzyme-linked immunosorbent assay (ELISA) were used to measure the level of IL-6 and P65, and phospho-p65 was measured by western blot.
33177483	4	78	theme	IBD	488:490	arg1	model					479:483	The mouse model	469:483	METHODS The mouse model of IBD	461:490	MATERIAL AND METHODS The mouse model of IBD was developed using DSS (4%) administered via the drinking water.
33177483	8	79	theme	16S	1119:1121	arg1	sequencing					1133:1142	16S rRNA gene sequencing	1119:1142	16S rRNA gene sequencing	1119:1142	16S rRNA gene sequencing was used to analyze fecal samples.
33177483	11	80	theme	IBD	1627:1629	arg1	model					1618:1622	a mouse model	1610:1622	a mouse model of IBD	1610:1629	CONCLUSIONS In a mouse model of IBD, treatment with vitamin D3 significantly increased the metabolism of rifaximin and reduced its therapeutic effects.
33177483	11	80	theme	IBD	1627:1629	arg1	treatment					1632:1640	treatment	1632:1640	treatment with vitamin D3	1632:1656	CONCLUSIONS In a mouse model of IBD, treatment with vitamin D3 significantly increased the metabolism of rifaximin and reduced its therapeutic effects.
33177483	7	81	theme	necrosis	918:925	arg1	TNF-alpha					941:949	TNF-alpha	941:949	TNF-alpha	941:949	Serum levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1ß (IL-1ß) and enzyme-linked immunosorbent assay (ELISA) were used to measure the level of IL-6 and P65, and phospho-p65 was measured by western blot.
33177483	7	81	theme	necrosis	918:925	arg1	factor-alpha					927:938	tumor necrosis factor-alpha	912:938	tumor necrosis factor-alpha (TNF-alpha)	912:950	Serum levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1ß (IL-1ß) and enzyme-linked immunosorbent assay (ELISA) were used to measure the level of IL-6 and P65, and phospho-p65 was measured by western blot.
33177483	3	82	theme	intestinal	353:362	arg1	flora					364:368	the intestinal flora	349:368	the intestinal flora of a dextran sulfate sodium (DSS)-induced mouse model treated with rifaximin	349:445	The purpose of this study was to assess the effect of vitamin D3 on the intestinal flora of a dextran sulfate sodium (DSS)-induced mouse model treated with rifaximin.
33177483	8	83	theme	gene	1128:1131	arg1	sequencing					1133:1142	16S rRNA gene sequencing	1119:1142	16S rRNA gene sequencing	1119:1142	16S rRNA gene sequencing was used to analyze fecal samples.
33177483	9	84	dep	RESULTS	1179:1185	arg1	reduced					1228:1234	reduced	1228:1234	reduced the inflammation severity of the colon	1228:1273	RESULTS In the DSS mouse model of IBD, rifaximin reduced the inflammation severity of the colon and reduced the expression of phospho-p65, p65, TNF-alpha, and IL-6.
33177483	9	84	dep	RESULTS	1179:1185	arg1	reduced					1279:1285	reduced	1279:1285	reduced the expression of phospho-p65, p65, TNF-alpha, and IL-6	1279:1341	RESULTS In the DSS mouse model of IBD, rifaximin reduced the inflammation severity of the colon and reduced the expression of phospho-p65, p65, TNF-alpha, and IL-6.
33177483	8	85	used	used	1148:1151	arg2	sequencing					1133:1142	16S rRNA gene sequencing	1119:1142	16S rRNA gene sequencing	1119:1142	16S rRNA gene sequencing was used to analyze fecal samples.
33177483	5	86	with	group	697:701	arg1	N=6					683:685	N=6	683:685	N=6	683:685	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	5	86	with	group	697:701	arg1	N=6					643:645	N=6	643:645	N=6	643:645	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	5	86	with	group	697:701	arg1	diet					717:720	a normal diet	708:720	a normal diet	708:720	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	5	86	with	group	697:701	arg1	diet					677:680	a normal diet	668:680	a normal diet (N=6)	668:686	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	5	86	with	group	697:701	arg1	diet					784:787	a normal diet	775:787	a normal diet	775:787	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	5	86	with	group	697:701	arg1	diet					637:640	a normal diet	628:640	a normal diet (N=6)	628:646	Twenty-four male C57BL6 mice were divided into the control group with a normal diet (N=6), the DSS group with a normal diet (N=6), the DSS group with a normal diet treated with rifaximin (N=6), and the DSS group with a normal diet treated with rifaximin and vitamin D3 (N=6).
33177483	3	87	theme	-induced	403:410	arg1	model					418:422	a dextran sulfate sodium (DSS)-induced mouse model	373:422	a dextran sulfate sodium (DSS)-induced mouse model treated with rifaximin	373:445	The purpose of this study was to assess the effect of vitamin D3 on the intestinal flora of a dextran sulfate sodium (DSS)-induced mouse model treated with rifaximin.
33177483	9	88	theme	IL-6	1338:1341	arg1	expression					1291:1300	the expression	1287:1300	the expression of phospho-p65, p65, TNF-alpha, and IL-6	1287:1341	RESULTS In the DSS mouse model of IBD, rifaximin reduced the inflammation severity of the colon and reduced the expression of phospho-p65, p65, TNF-alpha, and IL-6.
33177483	8	89	theme	fecal	1164:1168	arg1	samples					1170:1176	fecal samples	1164:1176	fecal samples	1164:1176	16S rRNA gene sequencing was used to analyze fecal samples.
32771947	8	0	theme	intestinal	1041:1050	arg1	flora					1052:1056	intestinal flora	1041:1056	intestinal flora	1041:1056	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	3	1	theme	gavage	591:596	arg1	administration					598:611	gavage administration	591:611	gavage administration three times one week	591:632	Mice in treatment group received CB (2 × 108 CFU in 200 ul normal saline) by gavage administration three times one week.
32771947	7	2	theme	Bcl-2	892:896	arg1	Expression					878:887	Expression	878:887	Expression of Bcl-2, Bax and the state of components of NF-κB signaling pathway	878:956	Expression of Bcl-2, Bax and the state of components of NF-κB signaling pathway were detected by western blot.
32771947	9	3	theme	NF-κB	1649:1653	arg1	pathway					1655:1661	NF-κB pathway	1649:1661	NF-κB pathway	1649:1661	In conclusion, CB could regulate structure and composition of gut microbiota and reduces colitis associated colon cancer in mice, the mechanism may be inhibiting NF-κB pathway and promoting apoptosis.
32771947	7	4	theme	NF-κB	934:938	arg1	pathway					950:956	NF-κB signaling pathway	934:956	NF-κB signaling pathway	934:956	Expression of Bcl-2, Bax and the state of components of NF-κB signaling pathway were detected by western blot.
32771947	1	5	theme	mice.6-8 weeks	218:231	arg1	mice					245:248	mice.6-8 weeks old C57BL/6 mice	218:248	mice.6-8 weeks old C57BL/6 mice	218:248	We investigate the effects of Clostridium butyricum(CB) on gut microbiota and colitis associated colon cancer(CAC) in mice.6-8 weeks old C57BL/6 mice were randomly divided into control, azoxymethane (AOM) + dextran sodium sulphate (DSS) and AOM + DSS + CB groups.
32771947	8	6	theme	COX-2	1386:1390	arg1	colitis					1217:1223	decreased colitis	1207:1223	decreased colitis	1207:1223	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	6	theme	COX-2	1386:1390	arg1	abundance					1182:1190	the relative abundance	1169:1190	the relative abundance of probiotics	1169:1204	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	6	theme	COX-2	1386:1390	arg1	expression					1468:1477	increased expression	1458:1477	increased expression of Bax	1458:1484	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	6	theme	COX-2	1386:1390	arg1	level					1439:1443	decreased level	1429:1443	decreased level of Bcl-2	1429:1452	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	6	theme	COX-2	1386:1390	arg1	phosphorylation					1403:1417	decreased phosphorylation	1393:1417	decreased phosphorylation of NF-κB	1393:1426	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	6	theme	COX-2	1386:1390	arg1	cytokines					1331:1339	decreased cytokines	1321:1339	decreased cytokines including TNF-a and IL-6	1321:1364	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	6	theme	COX-2	1386:1390	arg1	level					1377:1381	decreased level	1367:1381	decreased level of COX-2	1367:1390	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	1	7	theme	 + dextran	304:313	arg1	DSS					332:334	DSS	332:334	DSS	332:334	We investigate the effects of Clostridium butyricum(CB) on gut microbiota and colitis associated colon cancer(CAC) in mice.6-8 weeks old C57BL/6 mice were randomly divided into control, azoxymethane (AOM) + dextran sodium sulphate (DSS) and AOM + DSS + CB groups.
32771947	1	7	theme	 + dextran	304:313	arg1	sulphate					322:329	azoxymethane (AOM) + dextran sodium sulphate	286:329	azoxymethane (AOM) + dextran sodium sulphate (DSS)	286:335	We investigate the effects of Clostridium butyricum(CB) on gut microbiota and colitis associated colon cancer(CAC) in mice.6-8 weeks old C57BL/6 mice were randomly divided into control, azoxymethane (AOM) + dextran sodium sulphate (DSS) and AOM + DSS + CB groups.
32771947	8	8	theme	decreased	1393:1401	arg1	phosphorylation					1403:1417	decreased phosphorylation	1393:1417	decreased phosphorylation of NF-κB	1393:1426	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	1	9	theme	old	233:235	arg1	mice					245:248	mice.6-8 weeks old C57BL/6 mice	218:248	mice.6-8 weeks old C57BL/6 mice	218:248	We investigate the effects of Clostridium butyricum(CB) on gut microbiota and colitis associated colon cancer(CAC) in mice.6-8 weeks old C57BL/6 mice were randomly divided into control, azoxymethane (AOM) + dextran sodium sulphate (DSS) and AOM + DSS + CB groups.
32771947	1	10	theme	sodium	315:320	arg1	DSS					332:334	DSS	332:334	DSS	332:334	We investigate the effects of Clostridium butyricum(CB) on gut microbiota and colitis associated colon cancer(CAC) in mice.6-8 weeks old C57BL/6 mice were randomly divided into control, azoxymethane (AOM) + dextran sodium sulphate (DSS) and AOM + DSS + CB groups.
32771947	1	10	theme	sodium	315:320	arg1	sulphate					322:329	azoxymethane (AOM) + dextran sodium sulphate	286:329	azoxymethane (AOM) + dextran sodium sulphate (DSS)	286:335	We investigate the effects of Clostridium butyricum(CB) on gut microbiota and colitis associated colon cancer(CAC) in mice.6-8 weeks old C57BL/6 mice were randomly divided into control, azoxymethane (AOM) + dextran sodium sulphate (DSS) and AOM + DSS + CB groups.
32771947	1	11	theme	C57BL/6	237:243	arg1	mice					245:248	mice.6-8 weeks old C57BL/6 mice	218:248	mice.6-8 weeks old C57BL/6 mice	218:248	We investigate the effects of Clostridium butyricum(CB) on gut microbiota and colitis associated colon cancer(CAC) in mice.6-8 weeks old C57BL/6 mice were randomly divided into control, azoxymethane (AOM) + dextran sodium sulphate (DSS) and AOM + DSS + CB groups.
32771947	8	12	theme	NF-κB	1422:1426	arg1	colitis					1217:1223	decreased colitis	1207:1223	decreased colitis	1207:1223	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	12	theme	NF-κB	1422:1426	arg1	abundance					1182:1190	the relative abundance	1169:1190	the relative abundance of probiotics	1169:1204	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	12	theme	NF-κB	1422:1426	arg1	expression					1468:1477	increased expression	1458:1477	increased expression of Bax	1458:1484	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	12	theme	NF-κB	1422:1426	arg1	level					1439:1443	decreased level	1429:1443	decreased level of Bcl-2	1429:1452	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	12	theme	NF-κB	1422:1426	arg1	phosphorylation					1403:1417	decreased phosphorylation	1393:1417	decreased phosphorylation of NF-κB	1393:1426	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	12	theme	NF-κB	1422:1426	arg1	cytokines					1331:1339	decreased cytokines	1321:1339	decreased cytokines including TNF-a and IL-6	1321:1364	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	12	theme	NF-κB	1422:1426	arg1	level					1377:1381	decreased level	1367:1381	decreased level of COX-2	1367:1390	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	9	13	theme	gut	1549:1551	arg1	microbiota					1553:1562	gut microbiota	1549:1562	gut microbiota	1549:1562	In conclusion, CB could regulate structure and composition of gut microbiota and reduces colitis associated colon cancer in mice, the mechanism may be inhibiting NF-κB pathway and promoting apoptosis.
32771947	8	14	theme	relative	1173:1180	arg1	abundance					1182:1190	the relative abundance	1169:1190	the relative abundance of probiotics	1169:1204	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	15	from	ratio	1133:1137	arg1	level					1149:1153	phylum level	1142:1153	phylum level	1142:1153	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	9	16	theme	microbiota	1553:1562	arg1	composition					1534:1544	composition	1534:1544	composition	1534:1544	In conclusion, CB could regulate structure and composition of gut microbiota and reduces colitis associated colon cancer in mice, the mechanism may be inhibiting NF-κB pathway and promoting apoptosis.
32771947	9	16	theme	microbiota	1553:1562	arg1	structure					1520:1528	structure	1520:1528	structure	1520:1528	In conclusion, CB could regulate structure and composition of gut microbiota and reduces colitis associated colon cancer in mice, the mechanism may be inhibiting NF-κB pathway and promoting apoptosis.
32771947	8	17	theme	cancer	1269:1274	arg1	size					1250:1253	size	1250:1253	size	1250:1253	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	17	theme	cancer	1269:1274	arg1	incidence					1236:1244	incidence	1236:1244	incidence	1236:1244	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	5	18	theme	colitis	763:769	arg1	severity					751:758	severity	751:758	severity of colitis and tumorigenesis	751:787	Colon samples were collected to examine severity of colitis and tumorigenesis.
32771947	3	19	from	CFU	559:561	arg1	saline					580:585	200 ul normal saline	566:585	200 ul normal saline	566:585	Mice in treatment group received CB (2 × 108 CFU in 200 ul normal saline) by gavage administration three times one week.
32771947	8	20	theme	decreased	1429:1437	arg1	level					1439:1443	decreased level	1429:1443	decreased level of Bcl-2	1429:1452	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	21	theme	Bacteroidetes	1119:1131	arg1	ratio					1133:1137	Firmicutes/ Bacteroidetes ratio	1107:1137	Firmicutes/ Bacteroidetes ratio in phylum level	1107:1153	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	22	theme	microbial	1074:1082	arg1	composition					1084:1094	the microbial composition	1070:1094	the microbial composition	1070:1094	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	1	23	theme	gut	159:161	arg1	microbiota					163:172	gut microbiota	159:172	gut microbiota	159:172	We investigate the effects of Clostridium butyricum(CB) on gut microbiota and colitis associated colon cancer(CAC) in mice.6-8 weeks old C57BL/6 mice were randomly divided into control, azoxymethane (AOM) + dextran sodium sulphate (DSS) and AOM + DSS + CB groups.
32771947	5	24	theme	Colon	711:715	arg1	samples					717:723	Colon samples	711:723	Colon samples	711:723	Colon samples were collected to examine severity of colitis and tumorigenesis.
32771947	8	25	theme	cells	1314:1318	arg1	apoptosis					1295:1303	apoptosis	1295:1303	apoptosis of tumor cells	1295:1318	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	7	26	theme	western	975:981	arg1	blot					983:986	western blot	975:986	western blot	975:986	Expression of Bcl-2, Bax and the state of components of NF-κB signaling pathway were detected by western blot.
32771947	5	27	theme	tumorigenesis	775:787	arg1	severity					751:758	severity	751:758	severity of colitis and tumorigenesis	751:787	Colon samples were collected to examine severity of colitis and tumorigenesis.
32771947	0	28	theme	Clostridium	0:10	arg1	butyricum					12:20	Clostridium butyricum	0:20	Clostridium butyricum	0:20	Clostridium butyricum modulates gut microbiota and reduces colitis associated colon cancer in mice.
32771947	9	29	theme	colitis	1576:1582	arg1	cancer					1601:1606	colitis associated colon cancer	1576:1606	colitis associated colon cancer in mice	1576:1614	In conclusion, CB could regulate structure and composition of gut microbiota and reduces colitis associated colon cancer in mice, the mechanism may be inhibiting NF-κB pathway and promoting apoptosis.
32771947	3	30	dep	times	619:623	arg1	week					629:632	one week	625:632	gavage administration three times one week	591:632	Mice in treatment group received CB (2 × 108 CFU in 200 ul normal saline) by gavage administration three times one week.
32771947	8	31	theme	decreased	1367:1375	arg1	level					1377:1381	decreased level	1367:1381	decreased level of COX-2	1367:1390	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	1	32	theme	AOM + DSS + CB	341:354	arg1	groups					356:361	AOM + DSS + CB groups	341:361	AOM + DSS + CB groups	341:361	We investigate the effects of Clostridium butyricum(CB) on gut microbiota and colitis associated colon cancer(CAC) in mice.6-8 weeks old C57BL/6 mice were randomly divided into control, azoxymethane (AOM) + dextran sodium sulphate (DSS) and AOM + DSS + CB groups.
32771947	0	33	theme	gut	32:34	arg1	microbiota					36:45	gut microbiota	32:45	gut microbiota	32:45	Clostridium butyricum modulates gut microbiota and reduces colitis associated colon cancer in mice.
32771947	9	34	theme	associated	1584:1593	arg1	cancer					1601:1606	colitis associated colon cancer	1576:1606	colitis associated colon cancer in mice	1576:1614	In conclusion, CB could regulate structure and composition of gut microbiota and reduces colitis associated colon cancer in mice, the mechanism may be inhibiting NF-κB pathway and promoting apoptosis.
32771947	1	35	from	effects	119:125	arg1	microbiota					163:172	gut microbiota	159:172	gut microbiota	159:172	We investigate the effects of Clostridium butyricum(CB) on gut microbiota and colitis associated colon cancer(CAC) in mice.6-8 weeks old C57BL/6 mice were randomly divided into control, azoxymethane (AOM) + dextran sodium sulphate (DSS) and AOM + DSS + CB groups.
32771947	1	35	from	effects	119:125	arg1	colitis					178:184	colitis	178:184	colitis	178:184	We investigate the effects of Clostridium butyricum(CB) on gut microbiota and colitis associated colon cancer(CAC) in mice.6-8 weeks old C57BL/6 mice were randomly divided into control, azoxymethane (AOM) + dextran sodium sulphate (DSS) and AOM + DSS + CB groups.
32771947	2	36	theme	latter	376:381	arg1	groups					387:392	the latter two groups	372:392	the latter two groups	372:392	Mice in the latter two groups received an intraperitoneal injection of AOM (12.5 mg/kg), followed by three cycles of DSS diluted in water (2.5% w/v).
32771947	7	37	theme	Bax	899:901	arg1	Expression					878:887	Expression	878:887	Expression of Bcl-2, Bax and the state of components of NF-κB signaling pathway	878:956	Expression of Bcl-2, Bax and the state of components of NF-κB signaling pathway were detected by western blot.
32771947	7	38	theme	pathway	950:956	arg1	components					920:929	components	920:929	components of NF-κB signaling pathway	920:956	Expression of Bcl-2, Bax and the state of components of NF-κB signaling pathway were detected by western blot.
32771947	9	39	theme	colon	1595:1599	arg1	cancer					1601:1606	colitis associated colon cancer	1576:1606	colitis associated colon cancer in mice	1576:1614	In conclusion, CB could regulate structure and composition of gut microbiota and reduces colitis associated colon cancer in mice, the mechanism may be inhibiting NF-κB pathway and promoting apoptosis.
32771947	4	40	theme	Microbiota	635:644	arg1	composition					646:656	Microbiota composition	635:656	Microbiota composition	635:656	Microbiota composition was assessed by 16S rRNA high-throughput sequencing.
32771947	2	41	theme	AOM	435:437	arg1	injection					422:430	an intraperitoneal injection	403:430	an intraperitoneal injection of AOM (12.5 mg/kg)	403:450	Mice in the latter two groups received an intraperitoneal injection of AOM (12.5 mg/kg), followed by three cycles of DSS diluted in water (2.5% w/v).
32771947	8	42	theme	Bcl-2	1448:1452	arg1	colitis					1217:1223	decreased colitis	1207:1223	decreased colitis	1207:1223	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	42	theme	Bcl-2	1448:1452	arg1	abundance					1182:1190	the relative abundance	1169:1190	the relative abundance of probiotics	1169:1204	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	42	theme	Bcl-2	1448:1452	arg1	expression					1468:1477	increased expression	1458:1477	increased expression of Bax	1458:1484	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	42	theme	Bcl-2	1448:1452	arg1	level					1439:1443	decreased level	1429:1443	decreased level of Bcl-2	1429:1452	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	42	theme	Bcl-2	1448:1452	arg1	phosphorylation					1403:1417	decreased phosphorylation	1393:1417	decreased phosphorylation of NF-κB	1393:1426	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	42	theme	Bcl-2	1448:1452	arg1	cytokines					1331:1339	decreased cytokines	1321:1339	decreased cytokines including TNF-a and IL-6	1321:1364	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	42	theme	Bcl-2	1448:1452	arg1	level					1377:1381	decreased level	1367:1381	decreased level of COX-2	1367:1390	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	2	43	theme	DSS	481:483	arg1	cycles					471:476	three cycles	465:476	three cycles of DSS diluted in water (2.5% w/v)	465:511	Mice in the latter two groups received an intraperitoneal injection of AOM (12.5 mg/kg), followed by three cycles of DSS diluted in water (2.5% w/v).
32771947	0	44	from	cancer	84:89	arg1	mice					94:97	mice	94:97	mice	94:97	Clostridium butyricum modulates gut microbiota and reduces colitis associated colon cancer in mice.
32771947	8	45	theme	Bax	1482:1484	arg1	colitis					1217:1223	decreased colitis	1207:1223	decreased colitis	1207:1223	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	45	theme	Bax	1482:1484	arg1	abundance					1182:1190	the relative abundance	1169:1190	the relative abundance of probiotics	1169:1204	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	45	theme	Bax	1482:1484	arg1	expression					1468:1477	increased expression	1458:1477	increased expression of Bax	1458:1484	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	45	theme	Bax	1482:1484	arg1	level					1439:1443	decreased level	1429:1443	decreased level of Bcl-2	1429:1452	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	45	theme	Bax	1482:1484	arg1	phosphorylation					1403:1417	decreased phosphorylation	1393:1417	decreased phosphorylation of NF-κB	1393:1426	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	45	theme	Bax	1482:1484	arg1	cytokines					1331:1339	decreased cytokines	1321:1339	decreased cytokines including TNF-a and IL-6	1321:1364	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	45	theme	Bax	1482:1484	arg1	level					1377:1381	decreased level	1367:1381	decreased level of COX-2	1367:1390	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	3	46	from	Mice	514:517	arg1	group					532:536	treatment group	522:536	treatment group	522:536	Mice in treatment group received CB (2 × 108 CFU in 200 ul normal saline) by gavage administration three times one week.
32771947	1	47	theme	colon	197:201	arg1	CAC					210:212	CAC	210:212	CAC	210:212	We investigate the effects of Clostridium butyricum(CB) on gut microbiota and colitis associated colon cancer(CAC) in mice.6-8 weeks old C57BL/6 mice were randomly divided into control, azoxymethane (AOM) + dextran sodium sulphate (DSS) and AOM + DSS + CB groups.
32771947	1	47	theme	colon	197:201	arg1	cancer					203:208	colon cancer	197:208	colon cancer(CAC) in mice.6-8 weeks old C57BL/6 mice	197:248	We investigate the effects of Clostridium butyricum(CB) on gut microbiota and colitis associated colon cancer(CAC) in mice.6-8 weeks old C57BL/6 mice were randomly divided into control, azoxymethane (AOM) + dextran sodium sulphate (DSS) and AOM + DSS + CB groups.
32771947	8	48	theme	Firmicutes/	1107:1117	arg1	ratio					1133:1137	Firmicutes/ Bacteroidetes ratio	1107:1137	Firmicutes/ Bacteroidetes ratio in phylum level	1107:1153	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	3	49	dep	administration	598:611	arg1	times					619:623	three times	613:623	gavage administration three times one week	591:632	Mice in treatment group received CB (2 × 108 CFU in 200 ul normal saline) by gavage administration three times one week.
32771947	7	50	theme	state	911:915	arg1	Expression					878:887	Expression	878:887	Expression of Bcl-2, Bax and the state of components of NF-κB signaling pathway	878:956	Expression of Bcl-2, Bax and the state of components of NF-κB signaling pathway were detected by western blot.
32771947	8	51	theme	decreased	1207:1215	arg1	colitis					1217:1223	decreased colitis	1207:1223	decreased colitis	1207:1223	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	0	52	theme	colitis	59:65	arg1	cancer					84:89	colitis associated colon cancer	59:89	colitis associated colon cancer in mice	59:97	Clostridium butyricum modulates gut microbiota and reduces colitis associated colon cancer in mice.
32771947	8	53	theme	increased	1458:1466	arg1	expression					1468:1477	increased expression	1458:1477	increased expression of Bax	1458:1484	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	2	54	theme	%	506:506	arg1	w/v					508:510	2.5% w/v	503:510	2.5% w/v	503:510	Mice in the latter two groups received an intraperitoneal injection of AOM (12.5 mg/kg), followed by three cycles of DSS diluted in water (2.5% w/v).
32771947	2	54	theme	%	506:506	arg1	water					496:500	water	496:500	water (2.5% w/v)	496:511	Mice in the latter two groups received an intraperitoneal injection of AOM (12.5 mg/kg), followed by three cycles of DSS diluted in water (2.5% w/v).
32771947	8	55	theme	tumor	1308:1312	arg1	cells					1314:1318	tumor cells	1308:1318	tumor cells	1308:1318	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	1	56	from	cancer	203:208	arg1	mice					245:248	mice.6-8 weeks old C57BL/6 mice	218:248	mice.6-8 weeks old C57BL/6 mice	218:248	We investigate the effects of Clostridium butyricum(CB) on gut microbiota and colitis associated colon cancer(CAC) in mice.6-8 weeks old C57BL/6 mice were randomly divided into control, azoxymethane (AOM) + dextran sodium sulphate (DSS) and AOM + DSS + CB groups.
32771947	2	57	theme	intraperitoneal	406:420	arg1	injection					422:430	an intraperitoneal injection	403:430	an intraperitoneal injection of AOM (12.5 mg/kg)	403:450	Mice in the latter two groups received an intraperitoneal injection of AOM (12.5 mg/kg), followed by three cycles of DSS diluted in water (2.5% w/v).
32771947	1	58	theme	azoxymethane	286:297	arg1	DSS					332:334	DSS	332:334	DSS	332:334	We investigate the effects of Clostridium butyricum(CB) on gut microbiota and colitis associated colon cancer(CAC) in mice.6-8 weeks old C57BL/6 mice were randomly divided into control, azoxymethane (AOM) + dextran sodium sulphate (DSS) and AOM + DSS + CB groups.
32771947	1	58	theme	azoxymethane	286:297	arg1	sulphate					322:329	azoxymethane (AOM) + dextran sodium sulphate	286:329	azoxymethane (AOM) + dextran sodium sulphate (DSS)	286:335	We investigate the effects of Clostridium butyricum(CB) on gut microbiota and colitis associated colon cancer(CAC) in mice.6-8 weeks old C57BL/6 mice were randomly divided into control, azoxymethane (AOM) + dextran sodium sulphate (DSS) and AOM + DSS + CB groups.
32771947	7	59	theme	signaling	940:948	arg1	pathway					950:956	NF-κB signaling pathway	934:956	NF-κB signaling pathway	934:956	Expression of Bcl-2, Bax and the state of components of NF-κB signaling pathway were detected by western blot.
32771947	3	60	theme	ul	570:571	arg1	saline					580:585	200 ul normal saline	566:585	200 ul normal saline	566:585	Mice in treatment group received CB (2 × 108 CFU in 200 ul normal saline) by gavage administration three times one week.
32771947	8	61	dep	colitis	1217:1223	arg1	decreased					1226:1234	decreased	1226:1234	decreased incidence and size of colorectal cancer(CRC)	1226:1279	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	61	dep	colitis	1217:1223	arg1	increased					1285:1293	increased	1285:1293	increased apoptosis of tumor cells	1285:1318	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	62	theme	probiotics	1195:1204	arg1	colitis					1217:1223	decreased colitis	1207:1223	decreased colitis	1207:1223	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	62	theme	probiotics	1195:1204	arg1	abundance					1182:1190	the relative abundance	1169:1190	the relative abundance of probiotics	1169:1204	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	62	theme	probiotics	1195:1204	arg1	expression					1468:1477	increased expression	1458:1477	increased expression of Bax	1458:1484	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	62	theme	probiotics	1195:1204	arg1	level					1439:1443	decreased level	1429:1443	decreased level of Bcl-2	1429:1452	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	62	theme	probiotics	1195:1204	arg1	phosphorylation					1403:1417	decreased phosphorylation	1393:1417	decreased phosphorylation of NF-κB	1393:1426	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	62	theme	probiotics	1195:1204	arg1	cytokines					1331:1339	decreased cytokines	1321:1339	decreased cytokines including TNF-a and IL-6	1321:1364	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	62	theme	probiotics	1195:1204	arg1	level					1377:1381	decreased level	1367:1381	decreased level of COX-2	1367:1390	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	63	theme	decreased	1321:1329	arg1	cytokines					1331:1339	decreased cytokines	1321:1339	decreased cytokines including TNF-a and IL-6	1321:1364	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	7	64	theme	components	920:929	arg1	Bax					899:901	Bax	899:901	Bax	899:901	Expression of Bcl-2, Bax and the state of components of NF-κB signaling pathway were detected by western blot.
32771947	7	64	theme	components	920:929	arg1	state					911:915	the state	907:915	the state of components of NF-κB signaling pathway	907:956	Expression of Bcl-2, Bax and the state of components of NF-κB signaling pathway were detected by western blot.
32771947	7	64	theme	components	920:929	arg1	Bcl-2					892:896	Bcl-2	892:896	Bcl-2	892:896	Expression of Bcl-2, Bax and the state of components of NF-κB signaling pathway were detected by western blot.
32771947	3	65	theme	normal	573:578	arg1	saline					580:585	200 ul normal saline	566:585	200 ul normal saline	566:585	Mice in treatment group received CB (2 × 108 CFU in 200 ul normal saline) by gavage administration three times one week.
32771947	4	66	theme	high-throughput	683:697	arg1	sequencing					699:708	rRNA high-throughput sequencing	678:708	rRNA high-throughput sequencing	678:708	Microbiota composition was assessed by 16S rRNA high-throughput sequencing.
32771947	3	67	dep	CB	547:548	arg1	CFU					559:561	2 × 108 CFU	551:561	2 × 108 CFU in 200 ul normal saline	551:585	Mice in treatment group received CB (2 × 108 CFU in 200 ul normal saline) by gavage administration three times one week.
32771947	8	68	theme	colorectal	1258:1267	arg1	CRC					1276:1278	CRC	1276:1278	CRC	1276:1278	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	8	68	theme	colorectal	1258:1267	arg1	cancer					1269:1274	colorectal cancer	1258:1274	colorectal cancer(CRC)	1258:1279	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	9	69	from	cancer	1601:1606	arg1	mice					1611:1614	mice	1611:1614	mice	1611:1614	In conclusion, CB could regulate structure and composition of gut microbiota and reduces colitis associated colon cancer in mice, the mechanism may be inhibiting NF-κB pathway and promoting apoptosis.
32771947	4	70	theme	rRNA	678:681	arg1	sequencing					699:708	rRNA high-throughput sequencing	678:708	rRNA high-throughput sequencing	678:708	Microbiota composition was assessed by 16S rRNA high-throughput sequencing.
32771947	1	71	theme	Clostridium	130:140	arg1	CB					152:153	CB	152:153	CB	152:153	We investigate the effects of Clostridium butyricum(CB) on gut microbiota and colitis associated colon cancer(CAC) in mice.6-8 weeks old C57BL/6 mice were randomly divided into control, azoxymethane (AOM) + dextran sodium sulphate (DSS) and AOM + DSS + CB groups.
32771947	1	71	theme	Clostridium	130:140	arg1	butyricum					142:150	Clostridium butyricum	130:150	Clostridium butyricum(CB)	130:154	We investigate the effects of Clostridium butyricum(CB) on gut microbiota and colitis associated colon cancer(CAC) in mice.6-8 weeks old C57BL/6 mice were randomly divided into control, azoxymethane (AOM) + dextran sodium sulphate (DSS) and AOM + DSS + CB groups.
32771947	0	72	theme	colon	78:82	arg1	cancer					84:89	colitis associated colon cancer	59:89	colitis associated colon cancer in mice	59:97	Clostridium butyricum modulates gut microbiota and reduces colitis associated colon cancer in mice.
32771947	3	73	theme	200	566:568	arg1	ul					570:571	ul	570:571	ul	570:571	Mice in treatment group received CB (2 × 108 CFU in 200 ul normal saline) by gavage administration three times one week.
32771947	2	74	from	Mice	364:367	arg1	groups					387:392	the latter two groups	372:392	the latter two groups	372:392	Mice in the latter two groups received an intraperitoneal injection of AOM (12.5 mg/kg), followed by three cycles of DSS diluted in water (2.5% w/v).
32771947	1	75	theme	butyricum	142:150	arg1	effects					119:125	the effects	115:125	the effects of Clostridium butyricum(CB) on gut microbiota and colitis associated colon cancer(CAC) in mice.6-8 weeks old C57BL/6 mice were randomly divided into control, azoxymethane (AOM) + dextran sodium sulphate (DSS) and AOM + DSS + CB groups	115:361	We investigate the effects of Clostridium butyricum(CB) on gut microbiota and colitis associated colon cancer(CAC) in mice.6-8 weeks old C57BL/6 mice were randomly divided into control, azoxymethane (AOM) + dextran sodium sulphate (DSS) and AOM + DSS + CB groups.
32771947	8	76	theme	phylum	1142:1147	arg1	level					1149:1153	phylum level	1142:1153	phylum level	1142:1153	The results revealed that CB regulated structure of intestinal flora and changed the microbial composition; decreased Firmicutes/ Bacteroidetes ratio in phylum level and increased the relative abundance of probiotics; decreased colitis, decreased incidence and size of colorectal cancer(CRC) and increased apoptosis of tumor cells; decreased cytokines including TNF-a and IL-6; decreased level of COX-2; decreased phosphorylation of NF-κB; decreased level of Bcl-2 and increased expression of Bax.
32771947	0	77	theme	associated	67:76	arg1	cancer					84:89	colitis associated colon cancer	59:89	colitis associated colon cancer in mice	59:97	Clostridium butyricum modulates gut microbiota and reduces colitis associated colon cancer in mice.
32771947	3	78	theme	treatment	522:530	arg1	group					532:536	treatment group	522:536	treatment group	522:536	Mice in treatment group received CB (2 × 108 CFU in 200 ul normal saline) by gavage administration three times one week.
33246101	4	0	theme	clinical	900:907	arg1	potential					909:917	clinical potential	900:917	clinical potential	900:917	Through the analysis of matrix structure, stiffness and composition, we unexpectedly revealed that even prior to the first clinical symptoms, the colon displays its own unique extracellular-matrix signature and found specific markers of clinical potential, which were also validated in human subjects.
33246101	1	1	theme	bowel	176:180	arg1	diseases					182:189	inflammatory bowel diseases	163:189	inflammatory bowel diseases	163:189	Identification of early processes leading to complex tissue pathologies, such as inflammatory bowel diseases, ‎poses a major scientific and clinical challenge that is imperative for improved diagnosis and treatment.
33246101	1	2	theme	improved	264:271	arg1	diagnosis					273:281	improved diagnosis	264:281	improved diagnosis	264:281	Identification of early processes leading to complex tissue pathologies, such as inflammatory bowel diseases, ‎poses a major scientific and clinical challenge that is imperative for improved diagnosis and treatment.
33246101	4	3	theme	composition	719:729	arg1	analysis					675:682	the analysis	671:682	the analysis of matrix structure, stiffness and composition	671:729	Through the analysis of matrix structure, stiffness and composition, we unexpectedly revealed that even prior to the first clinical symptoms, the colon displays its own unique extracellular-matrix signature and found specific markers of clinical potential, which were also validated in human subjects.
33246101	4	4	theme	clinical	786:793	arg1	symptoms					795:802	the first clinical symptoms	776:802	the first clinical symptoms	776:802	Through the analysis of matrix structure, stiffness and composition, we unexpectedly revealed that even prior to the first clinical symptoms, the colon displays its own unique extracellular-matrix signature and found specific markers of clinical potential, which were also validated in human subjects.
33246101	2	5	theme	inflammation	314:325	arg1	onset					327:331	inflammation onset	314:331	inflammation onset	314:331	Most studies of inflammation onset focus on cellular processes and signaling molecules, while overlooking the environment in which they take place, the continuously remodeled extracellular matrix.
33246101	3	6	theme	disease	588:594	arg1	onset					596:600	disease onset	588:600	disease onset	588:600	In this study, we used colitis models for investigating extracellular-matrix dynamics during disease onset, while treating the matrix as a complete and defined entity.
33246101	0	7	theme	Distinct	0:7	arg1	events					41:46	Distinct extracellular-matrix remodeling events	0:46	Distinct extracellular-matrix remodeling events	0:46	Distinct extracellular-matrix remodeling events precede symptoms of inflammation.
33246101	1	8	theme	tissue	135:140	arg1	pathologies					142:152	complex tissue pathologies	127:152	complex tissue pathologies	127:152	Identification of early processes leading to complex tissue pathologies, such as inflammatory bowel diseases, ‎poses a major scientific and clinical challenge that is imperative for improved diagnosis and treatment.
33246101	1	8	theme	tissue	135:140	arg1	diseases					182:189	inflammatory bowel diseases	163:189	inflammatory bowel diseases	163:189	Identification of early processes leading to complex tissue pathologies, such as inflammatory bowel diseases, ‎poses a major scientific and clinical challenge that is imperative for improved diagnosis and treatment.
33246101	2	9	theme	Most	298:301	arg1	studies					303:309	Most studies	298:309	Most studies of inflammation onset	298:331	Most studies of inflammation onset focus on cellular processes and signaling molecules, while overlooking the environment in which they take place, the continuously remodeled extracellular matrix.
33246101	4	10	theme	matrix	687:692	arg1	structure					694:702	matrix structure	687:702	matrix structure	687:702	Through the analysis of matrix structure, stiffness and composition, we unexpectedly revealed that even prior to the first clinical symptoms, the colon displays its own unique extracellular-matrix signature and found specific markers of clinical potential, which were also validated in human subjects.
33246101	1	11	theme	early	100:104	arg1	processes					106:114	early processes	100:114	early processes leading to complex tissue pathologies, such as inflammatory bowel diseases,	100:190	Identification of early processes leading to complex tissue pathologies, such as inflammatory bowel diseases, ‎poses a major scientific and clinical challenge that is imperative for improved diagnosis and treatment.
33246101	0	12	theme	extracellular-matrix	9:28	arg1	events					41:46	Distinct extracellular-matrix remodeling events	0:46	Distinct extracellular-matrix remodeling events	0:46	Distinct extracellular-matrix remodeling events precede symptoms of inflammation.
33246101	1	13	theme	processes	106:114	arg1	Identification					82:95	Identification	82:95	Identification of early processes leading to complex tissue pathologies, such as inflammatory bowel diseases,	82:190	Identification of early processes leading to complex tissue pathologies, such as inflammatory bowel diseases, ‎poses a major scientific and clinical challenge that is imperative for improved diagnosis and treatment.
33246101	2	14	theme	remodeled	463:471	arg1	place					439:443	place	439:443	place	439:443	Most studies of inflammation onset focus on cellular processes and signaling molecules, while overlooking the environment in which they take place, the continuously remodeled extracellular matrix.
33246101	2	14	theme	remodeled	463:471	arg1	matrix					487:492	the continuously remodeled extracellular matrix	446:492	the continuously remodeled extracellular matrix	446:492	Most studies of inflammation onset focus on cellular processes and signaling molecules, while overlooking the environment in which they take place, the continuously remodeled extracellular matrix.
33246101	4	15	theme	specific	880:887	arg1	markers					889:895	specific markers	880:895	specific markers	880:895	Through the analysis of matrix structure, stiffness and composition, we unexpectedly revealed that even prior to the first clinical symptoms, the colon displays its own unique extracellular-matrix signature and found specific markers of clinical potential, which were also validated in human subjects.
33246101	1	16	theme	major	201:205	arg1	imperative					249:258	imperative	249:258	imperative	249:258	Identification of early processes leading to complex tissue pathologies, such as inflammatory bowel diseases, ‎poses a major scientific and clinical challenge that is imperative for improved diagnosis and treatment.
33246101	1	16	theme	major	201:205	arg1	challenge					231:239	a major scientific and clinical challenge	199:239	a major scientific and clinical challenge that is imperative for improved diagnosis and treatment	199:295	Identification of early processes leading to complex tissue pathologies, such as inflammatory bowel diseases, ‎poses a major scientific and clinical challenge that is imperative for improved diagnosis and treatment.
33246101	4	17	theme	human	949:953	arg1	subjects					955:962	human subjects	949:962	human subjects	949:962	Through the analysis of matrix structure, stiffness and composition, we unexpectedly revealed that even prior to the first clinical symptoms, the colon displays its own unique extracellular-matrix signature and found specific markers of clinical potential, which were also validated in human subjects.
33246101	4	18	theme	extracellular-matrix	839:858	arg1	signature					860:868	its own unique extracellular-matrix signature	824:868	its own unique extracellular-matrix signature	824:868	Through the analysis of matrix structure, stiffness and composition, we unexpectedly revealed that even prior to the first clinical symptoms, the colon displays its own unique extracellular-matrix signature and found specific markers of clinical potential, which were also validated in human subjects.
33246101	2	19	theme	onset	327:331	arg1	studies					303:309	Most studies	298:309	Most studies of inflammation onset	298:331	Most studies of inflammation onset focus on cellular processes and signaling molecules, while overlooking the environment in which they take place, the continuously remodeled extracellular matrix.
33246101	1	20	theme	scientific	207:216	arg1	imperative					249:258	imperative	249:258	imperative	249:258	Identification of early processes leading to complex tissue pathologies, such as inflammatory bowel diseases, ‎poses a major scientific and clinical challenge that is imperative for improved diagnosis and treatment.
33246101	1	20	theme	scientific	207:216	arg1	challenge					231:239	a major scientific and clinical challenge	199:239	a major scientific and clinical challenge that is imperative for improved diagnosis and treatment	199:295	Identification of early processes leading to complex tissue pathologies, such as inflammatory bowel diseases, ‎poses a major scientific and clinical challenge that is imperative for improved diagnosis and treatment.
33246101	0	21	theme	remodeling	30:39	arg1	events					41:46	Distinct extracellular-matrix remodeling events	0:46	Distinct extracellular-matrix remodeling events	0:46	Distinct extracellular-matrix remodeling events precede symptoms of inflammation.
33246101	3	22	theme	extracellular-matrix	551:570	arg1	dynamics					572:579	extracellular-matrix dynamics	551:579	extracellular-matrix dynamics	551:579	In this study, we used colitis models for investigating extracellular-matrix dynamics during disease onset, while treating the matrix as a complete and defined entity.
33246101	1	23	theme	complex	127:133	arg1	pathologies					142:152	complex tissue pathologies	127:152	complex tissue pathologies	127:152	Identification of early processes leading to complex tissue pathologies, such as inflammatory bowel diseases, ‎poses a major scientific and clinical challenge that is imperative for improved diagnosis and treatment.
33246101	1	23	theme	complex	127:133	arg1	diseases					182:189	inflammatory bowel diseases	163:189	inflammatory bowel diseases	163:189	Identification of early processes leading to complex tissue pathologies, such as inflammatory bowel diseases, ‎poses a major scientific and clinical challenge that is imperative for improved diagnosis and treatment.
33246101	3	24	theme	colitis	518:524	arg1	models					526:531	colitis models	518:531	colitis models for investigating extracellular-matrix dynamics during disease onset	518:600	In this study, we used colitis models for investigating extracellular-matrix dynamics during disease onset, while treating the matrix as a complete and defined entity.
33246101	4	25	theme	unique	832:837	arg1	signature					860:868	its own unique extracellular-matrix signature	824:868	its own unique extracellular-matrix signature	824:868	Through the analysis of matrix structure, stiffness and composition, we unexpectedly revealed that even prior to the first clinical symptoms, the colon displays its own unique extracellular-matrix signature and found specific markers of clinical potential, which were also validated in human subjects.
33246101	4	26	theme	structure	694:702	arg1	analysis					675:682	the analysis	671:682	the analysis of matrix structure, stiffness and composition	671:729	Through the analysis of matrix structure, stiffness and composition, we unexpectedly revealed that even prior to the first clinical symptoms, the colon displays its own unique extracellular-matrix signature and found specific markers of clinical potential, which were also validated in human subjects.
33246101	1	27	theme	clinical	222:229	arg1	imperative					249:258	imperative	249:258	imperative	249:258	Identification of early processes leading to complex tissue pathologies, such as inflammatory bowel diseases, ‎poses a major scientific and clinical challenge that is imperative for improved diagnosis and treatment.
33246101	1	27	theme	clinical	222:229	arg1	challenge					231:239	a major scientific and clinical challenge	199:239	a major scientific and clinical challenge that is imperative for improved diagnosis and treatment	199:295	Identification of early processes leading to complex tissue pathologies, such as inflammatory bowel diseases, ‎poses a major scientific and clinical challenge that is imperative for improved diagnosis and treatment.
33246101	4	28	theme	first	780:784	arg1	symptoms					795:802	the first clinical symptoms	776:802	the first clinical symptoms	776:802	Through the analysis of matrix structure, stiffness and composition, we unexpectedly revealed that even prior to the first clinical symptoms, the colon displays its own unique extracellular-matrix signature and found specific markers of clinical potential, which were also validated in human subjects.
33246101	4	29	theme	own	828:830	arg1	signature					860:868	its own unique extracellular-matrix signature	824:868	its own unique extracellular-matrix signature	824:868	Through the analysis of matrix structure, stiffness and composition, we unexpectedly revealed that even prior to the first clinical symptoms, the colon displays its own unique extracellular-matrix signature and found specific markers of clinical potential, which were also validated in human subjects.
33246101	0	30	theme	inflammation	68:79	arg1	symptoms					56:63	symptoms	56:63	symptoms of inflammation	56:79	Distinct extracellular-matrix remodeling events precede symptoms of inflammation.
33246101	3	31	used	used	513:516	arg2	we					510:511	we	510:511	we	510:511	In this study, we used colitis models for investigating extracellular-matrix dynamics during disease onset, while treating the matrix as a complete and defined entity.
33246101	2	32	theme	signaling	365:373	arg1	molecules					375:383	signaling molecules	365:383	signaling molecules	365:383	Most studies of inflammation onset focus on cellular processes and signaling molecules, while overlooking the environment in which they take place, the continuously remodeled extracellular matrix.
33246101	5	33	theme	immune	1071:1076	arg1	cells					1078:1082	immune cells	1071:1082	immune cells bearing remodeling enzymes	1071:1109	We also show that the emergence of this pre-symptomatic matrix is mediated by subclinical infiltration of immune cells bearing remodeling enzymes.
33246101	1	34	theme	inflammatory	163:174	arg1	diseases					182:189	inflammatory bowel diseases	163:189	inflammatory bowel diseases	163:189	Identification of early processes leading to complex tissue pathologies, such as inflammatory bowel diseases, ‎poses a major scientific and clinical challenge that is imperative for improved diagnosis and treatment.
33246101	7	35	theme	wide	1329:1332	arg1	range					1334:1338	a wide range	1327:1338	a wide range of diseases	1327:1350	We suggest that the existence of a pre-symptomatic extracellular-matrix is general and relevant to a wide range of diseases.
33246101	5	36	theme	subclinical	1043:1053	arg1	infiltration					1055:1066	subclinical infiltration	1043:1066	subclinical infiltration of immune cells bearing remodeling enzymes	1043:1109	We also show that the emergence of this pre-symptomatic matrix is mediated by subclinical infiltration of immune cells bearing remodeling enzymes.
33246101	5	37	theme	cells	1078:1082	arg1	infiltration					1055:1066	subclinical infiltration	1043:1066	subclinical infiltration of immune cells bearing remodeling enzymes	1043:1109	We also show that the emergence of this pre-symptomatic matrix is mediated by subclinical infiltration of immune cells bearing remodeling enzymes.
33246101	3	38	theme	defined	647:653	arg1	matrix					622:627	the matrix	618:627	the matrix	618:627	In this study, we used colitis models for investigating extracellular-matrix dynamics during disease onset, while treating the matrix as a complete and defined entity.
33246101	3	38	theme	defined	647:653	arg1	entity					655:660	a complete and defined entity	632:660	a complete and defined entity	632:660	In this study, we used colitis models for investigating extracellular-matrix dynamics during disease onset, while treating the matrix as a complete and defined entity.
33246101	4	39	theme	stiffness	705:713	arg1	analysis					675:682	the analysis	671:682	the analysis of matrix structure, stiffness and composition	671:729	Through the analysis of matrix structure, stiffness and composition, we unexpectedly revealed that even prior to the first clinical symptoms, the colon displays its own unique extracellular-matrix signature and found specific markers of clinical potential, which were also validated in human subjects.
33246101	6	40	theme	matrix	1174:1179	arg1	signature					1181:1189	its matrix signature	1170:1189	its matrix signature	1170:1189	Remarkably, whether the inflammation is chronic or acute, its matrix signature converges at pre-symptomatic states.
33246101	5	41	theme	matrix	1021:1026	arg1	emergence					987:995	the emergence	983:995	the emergence of this pre-symptomatic matrix	983:1026	We also show that the emergence of this pre-symptomatic matrix is mediated by subclinical infiltration of immune cells bearing remodeling enzymes.
33246101	2	42	theme	cellular	342:349	arg1	processes					351:359	cellular processes	342:359	cellular processes	342:359	Most studies of inflammation onset focus on cellular processes and signaling molecules, while overlooking the environment in which they take place, the continuously remodeled extracellular matrix.
33246101	4	43	theme	potential	909:917	arg1	markers					889:895	specific markers	880:895	specific markers	880:895	Through the analysis of matrix structure, stiffness and composition, we unexpectedly revealed that even prior to the first clinical symptoms, the colon displays its own unique extracellular-matrix signature and found specific markers of clinical potential, which were also validated in human subjects.
33246101	5	44	theme	remodeling	1092:1101	arg1	enzymes					1103:1109	remodeling enzymes	1092:1109	remodeling enzymes	1092:1109	We also show that the emergence of this pre-symptomatic matrix is mediated by subclinical infiltration of immune cells bearing remodeling enzymes.
33246101	3	45	theme	complete	634:641	arg1	matrix					622:627	the matrix	618:627	the matrix	618:627	In this study, we used colitis models for investigating extracellular-matrix dynamics during disease onset, while treating the matrix as a complete and defined entity.
33246101	3	45	theme	complete	634:641	arg1	entity					655:660	a complete and defined entity	632:660	a complete and defined entity	632:660	In this study, we used colitis models for investigating extracellular-matrix dynamics during disease onset, while treating the matrix as a complete and defined entity.
33246101	7	46	theme	diseases	1343:1350	arg1	range					1334:1338	a wide range	1327:1338	a wide range of diseases	1327:1350	We suggest that the existence of a pre-symptomatic extracellular-matrix is general and relevant to a wide range of diseases.
33246101	6	47	theme	pre-symptomatic	1204:1218	arg1	states					1220:1225	pre-symptomatic states	1204:1225	pre-symptomatic states	1204:1225	Remarkably, whether the inflammation is chronic or acute, its matrix signature converges at pre-symptomatic states.
33246101	5	48	theme	pre-symptomatic	1005:1019	arg1	matrix					1021:1026	this pre-symptomatic matrix	1000:1026	this pre-symptomatic matrix	1000:1026	We also show that the emergence of this pre-symptomatic matrix is mediated by subclinical infiltration of immune cells bearing remodeling enzymes.
33246101	2	49	theme	extracellular	473:485	arg1	place					439:443	place	439:443	place	439:443	Most studies of inflammation onset focus on cellular processes and signaling molecules, while overlooking the environment in which they take place, the continuously remodeled extracellular matrix.
33246101	2	49	theme	extracellular	473:485	arg1	matrix					487:492	the continuously remodeled extracellular matrix	446:492	the continuously remodeled extracellular matrix	446:492	Most studies of inflammation onset focus on cellular processes and signaling molecules, while overlooking the environment in which they take place, the continuously remodeled extracellular matrix.
33246101	7	50	theme	extracellular-matrix	1279:1298	arg1	general					1303:1309	general	1303:1309	general	1303:1309	We suggest that the existence of a pre-symptomatic extracellular-matrix is general and relevant to a wide range of diseases.
33246101	7	50	theme	extracellular-matrix	1279:1298	arg1	existence					1248:1256	the existence	1244:1256	the existence of a pre-symptomatic extracellular-matrix	1244:1298	We suggest that the existence of a pre-symptomatic extracellular-matrix is general and relevant to a wide range of diseases.
31702533	2	0	theme	Gram-stain-positive	100:118	arg1	bacterium					180:188	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium	98:188	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium with peritrichous flagella	98:215	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium with peritrichous flagella was isolated from a gut sample of the larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
31702533	7	1	theme	only	1029:1032	arg1	MK-7					1057:1060	MK-7	1057:1060	MK-7	1057:1060	The only isoprenoid quinone was MK-7.
31702533	7	1	theme	only	1029:1032	arg1	quinone					1045:1051	The only isoprenoid quinone	1025:1051	The only isoprenoid quinone	1025:1051	The only isoprenoid quinone was MK-7.
31702533	8	2	theme	Polar	1063:1067	arg1	lipids					1069:1074	Polar lipids	1063:1074	Polar lipids of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid, two unidentified phospholipids and an unidentified lipid	1063:1241	Polar lipids of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid, two unidentified phospholipids and an unidentified lipid were present.
31702533	4	3	theme	highest	549:555	arg1	similarity					557:566	the highest similarity	545:566	the highest similarity	545:566	16S rRNA gene sequence comparisons indicated that strain FW100M-2T had the highest similarity to type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa.
31702533	11	4	theme	Paenibacillus	1471:1483	arg1	sp					1496:1497	the name Paenibacillus protaetiae sp	1462:1497	the name Paenibacillus protaetiae sp	1462:1497	Hence, strain FW100M-2T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus protaetiae sp.
31702533	5	5	theme	strain	788:793	arg1	FW100M-2T					795:803	strain FW100M-2T	788:803	strain FW100M-2T	788:803	The phylogenetic tree showed that strain FW100M-2T fell into the genus Paenibacillus, and formed a cluster with P. thailandensis S3-4AT independent from other Paenibacillus species.
31702533	8	6	theme	unidentified	1190:1201	arg1	phospholipids					1203:1215	two unidentified phospholipids	1186:1215	two unidentified phospholipids	1186:1215	Polar lipids of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid, two unidentified phospholipids and an unidentified lipid were present.
31702533	2	7	theme	National	303:310	arg1	Institute					312:320	the National Institute	299:320	the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea	299:375	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium with peritrichous flagella was isolated from a gut sample of the larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
31702533	12	8	theme	19327T=NBRC	1540:1550	arg1	113071T					1552:1558	=KACC 19327T=NBRC 113071T	1534:1558	=KACC 19327T=NBRC 113071T	1534:1558	nov. is proposed, with FW100M-2T (=KACC 19327T=NBRC 113071T) as the type strain.
31702533	12	8	theme	19327T=NBRC	1540:1550	arg1	FW100M-2T					1523:1531	FW100M-2T	1523:1531	FW100M-2T (=KACC 19327T=NBRC 113071T) as the type strain	1523:1578	nov. is proposed, with FW100M-2T (=KACC 19327T=NBRC 113071T) as the type strain.
31702533	4	9	theme	Paenibacillus	587:599	arg1	S3-4AT					615:620	Paenibacillus thailandensis S3-4AT	587:620	Paenibacillus thailandensis S3-4AT (96.8 %)	587:629	16S rRNA gene sequence comparisons indicated that strain FW100M-2T had the highest similarity to type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa.
31702533	4	9	theme	Paenibacillus	587:599	arg1	%					628:628	96.8 %	623:628	96.8 %	623:628	16S rRNA gene sequence comparisons indicated that strain FW100M-2T had the highest similarity to type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa.
31702533	5	10	theme	phylogenetic	758:769	arg1	tree					771:774	The phylogenetic tree	754:774	The phylogenetic tree	754:774	The phylogenetic tree showed that strain FW100M-2T fell into the genus Paenibacillus, and formed a cluster with P. thailandensis S3-4AT independent from other Paenibacillus species.
31702533	4	11	theme	1355T	667:671	arg1	strains					576:582	type strains	571:582	type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %)	571:680	16S rRNA gene sequence comparisons indicated that strain FW100M-2T had the highest similarity to type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa.
31702533	4	12	theme	S3-4AT	615:620	arg1	strains					576:582	type strains	571:582	type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %)	571:680	16S rRNA gene sequence comparisons indicated that strain FW100M-2T had the highest similarity to type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa.
31702533	9	13	located	present	1290:1296	arg2	present					1290:1296	present	1290:1296	present	1290:1296	The meso-diaminopimelic acid was present in the cell-wall peptidoglycan.
31702533	9	13	located	present	1290:1296	arg2	acid					1281:1284	The meso-diaminopimelic acid	1257:1284	The meso-diaminopimelic acid	1257:1284	The meso-diaminopimelic acid was present in the cell-wall peptidoglycan.
31702533	9	13	located	present	1290:1296	arg1	peptidoglycan					1315:1327	the cell-wall peptidoglycan	1301:1327	the cell-wall peptidoglycan	1301:1327	The meso-diaminopimelic acid was present in the cell-wall peptidoglycan.
31702533	4	14	contain	had	541:543	arg1	FW100M-2T					531:539	strain FW100M-2T	524:539	strain FW100M-2T	524:539	16S rRNA gene sequence comparisons indicated that strain FW100M-2T had the highest similarity to type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa.
31702533	4	14	contain	had	541:543	arg2	similarity					557:566	the highest similarity	545:566	the highest similarity	545:566	16S rRNA gene sequence comparisons indicated that strain FW100M-2T had the highest similarity to type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa.
31702533	5	15	theme	P.	866:867	arg1	S3-4AT					883:888	P. thailandensis S3-4AT	866:888	P. thailandensis S3-4AT independent from other Paenibacillus species	866:933	The phylogenetic tree showed that strain FW100M-2T fell into the genus Paenibacillus, and formed a cluster with P. thailandensis S3-4AT independent from other Paenibacillus species.
31702533	4	16	theme	Paenibacillus	635:647	arg1	1355T					667:671	Paenibacillus agaridevorans DSM 1355T	635:671	Paenibacillus agaridevorans DSM 1355T (96.3 %)	635:680	16S rRNA gene sequence comparisons indicated that strain FW100M-2T had the highest similarity to type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa.
31702533	4	16	theme	Paenibacillus	635:647	arg1	%					679:679	96.3 %	674:679	96.3 %	674:679	16S rRNA gene sequence comparisons indicated that strain FW100M-2T had the highest similarity to type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa.
31702533	11	17	theme	genus	1431:1435	arg1	species					1416:1422	a novel species	1408:1422	a novel species	1408:1422	Hence, strain FW100M-2T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus protaetiae sp.
31702533	4	18	theme	DSM	663:665	arg1	1355T					667:671	Paenibacillus agaridevorans DSM 1355T	635:671	Paenibacillus agaridevorans DSM 1355T (96.3 %)	635:680	16S rRNA gene sequence comparisons indicated that strain FW100M-2T had the highest similarity to type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa.
31702533	4	18	theme	DSM	663:665	arg1	%					679:679	96.3 %	674:679	96.3 %	674:679	16S rRNA gene sequence comparisons indicated that strain FW100M-2T had the highest similarity to type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa.
31702533	11	19	theme	name	1466:1469	arg1	sp					1496:1497	the name Paenibacillus protaetiae sp	1462:1497	the name Paenibacillus protaetiae sp	1462:1497	Hence, strain FW100M-2T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus protaetiae sp.
31702533	2	20	from	Institute	312:320	arg1	sample					241:246	a gut sample	235:246	a gut sample of the larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea	235:375	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium with peritrichous flagella was isolated from a gut sample of the larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
31702533	2	20	from	Institute	312:320	arg1	larva					255:259	the larva	251:259	the larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea	251:375	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium with peritrichous flagella was isolated from a gut sample of the larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
31702533	10	21	theme	G+C	1346:1348	arg1	content					1350:1356	The genomic DNA G+C content	1330:1356	The genomic DNA G+C content	1330:1356	The genomic DNA G+C content was 51.5 mol%.
31702533	10	21	theme	G+C	1346:1348	arg1	%					1370:1370	51.5 mol%	1362:1370	51.5 mol%	1362:1370	The genomic DNA G+C content was 51.5 mol%.
31702533	4	22	theme	similarity	700:709	arg1	values					711:716	sequence similarity values	691:716	sequence similarity values less than 96.0 %	691:733	16S rRNA gene sequence comparisons indicated that strain FW100M-2T had the highest similarity to type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa.
31702533	5	23	theme	independent	890:900	arg1	S3-4AT					883:888	P. thailandensis S3-4AT	866:888	P. thailandensis S3-4AT independent from other Paenibacillus species	866:933	The phylogenetic tree showed that strain FW100M-2T fell into the genus Paenibacillus, and formed a cluster with P. thailandensis S3-4AT independent from other Paenibacillus species.
31702533	4	24	theme	less	718:721	arg1	values					711:716	sequence similarity values	691:716	sequence similarity values less than 96.0 %	691:733	16S rRNA gene sequence comparisons indicated that strain FW100M-2T had the highest similarity to type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa.
31702533	5	25	theme	other	907:911	arg1	species					927:933	other Paenibacillus species	907:933	other Paenibacillus species	907:933	The phylogenetic tree showed that strain FW100M-2T fell into the genus Paenibacillus, and formed a cluster with P. thailandensis S3-4AT independent from other Paenibacillus species.
31702533	11	26	theme	strain	1380:1385	arg1	FW100M-2T					1387:1395	strain FW100M-2T	1380:1395	strain FW100M-2T	1380:1395	Hence, strain FW100M-2T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus protaetiae sp.
31702533	0	27	theme	Paenibacillus	0:12	arg1	sp					25:26	Paenibacillus protaetiae sp	0:26	Paenibacillus protaetiae sp.	0:27	Paenibacillus protaetiae sp.
31702533	1	28	theme	brevitarsis	75:85	arg1	seulensis					87:95	Protaetia brevitarsis seulensis	65:95	Protaetia brevitarsis seulensis	65:95	nov., isolated from gut of larva of Protaetia brevitarsis seulensis.
31702533	12	29	theme	type	1568:1571	arg1	strain					1573:1578	the type strain	1564:1578	the type strain	1564:1578	nov. is proposed, with FW100M-2T (=KACC 19327T=NBRC 113071T) as the type strain.
31702533	10	30	theme	DNA	1342:1344	arg1	content					1350:1356	The genomic DNA G+C content	1330:1356	The genomic DNA G+C content	1330:1356	The genomic DNA G+C content was 51.5 mol%.
31702533	10	30	theme	DNA	1342:1344	arg1	%					1370:1370	51.5 mol%	1362:1370	51.5 mol%	1362:1370	The genomic DNA G+C content was 51.5 mol%.
31702533	5	31	dep	P.	866:867	arg1	thailandensis					869:881	thailandensis	869:881	thailandensis	869:881	The phylogenetic tree showed that strain FW100M-2T fell into the genus Paenibacillus, and formed a cluster with P. thailandensis S3-4AT independent from other Paenibacillus species.
31702533	2	32	theme	motile	173:178	arg1	bacterium					180:188	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium	98:188	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium with peritrichous flagella	98:215	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium with peritrichous flagella was isolated from a gut sample of the larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
31702533	4	33	theme	other	742:746	arg1	taxa					748:751	all other taxa	738:751	all other taxa	738:751	16S rRNA gene sequence comparisons indicated that strain FW100M-2T had the highest similarity to type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa.
31702533	11	34	theme	novel	1410:1414	arg1	species					1416:1422	a novel species	1408:1422	a novel species	1408:1422	Hence, strain FW100M-2T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus protaetiae sp.
31702533	4	35	theme	16S	474:476	arg1	comparisons					497:507	16S rRNA gene sequence comparisons	474:507	16S rRNA gene sequence comparisons	474:507	16S rRNA gene sequence comparisons indicated that strain FW100M-2T had the highest similarity to type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa.
31702533	2	36	theme	endospore-forming	151:167	arg1	bacterium					180:188	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium	98:188	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium with peritrichous flagella	98:215	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium with peritrichous flagella was isolated from a gut sample of the larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
31702533	9	37	attach	present	1290:1296	arg2	present					1290:1296	present	1290:1296	present	1290:1296	The meso-diaminopimelic acid was present in the cell-wall peptidoglycan.
31702533	9	37	attach	present	1290:1296	arg2	acid					1281:1284	The meso-diaminopimelic acid	1257:1284	The meso-diaminopimelic acid	1257:1284	The meso-diaminopimelic acid was present in the cell-wall peptidoglycan.
31702533	9	37	attach	present	1290:1296	arg1	peptidoglycan					1315:1327	the cell-wall peptidoglycan	1301:1327	the cell-wall peptidoglycan	1301:1327	The meso-diaminopimelic acid was present in the cell-wall peptidoglycan.
31702533	4	38	theme	gene	483:486	arg1	comparisons					497:507	16S rRNA gene sequence comparisons	474:507	16S rRNA gene sequence comparisons	474:507	16S rRNA gene sequence comparisons indicated that strain FW100M-2T had the highest similarity to type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa.
31702533	6	39	theme	major	1006:1010	arg1	Antesio-C15 					936:947	Antesio-C15 	936:947	Antesio-C15 	936:947	Antesio-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0 were detected as the major fatty acids.
31702533	6	39	theme	major	1006:1010	arg1	acids					1018:1022	the major fatty acids	1002:1022	the major fatty acids	1002:1022	Antesio-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0 were detected as the major fatty acids.
31702533	4	40	theme	type	571:574	arg1	strains					576:582	type strains	571:582	type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %)	571:680	16S rRNA gene sequence comparisons indicated that strain FW100M-2T had the highest similarity to type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa.
31702533	2	41	theme	aerobic	142:148	arg1	bacterium					180:188	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium	98:188	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium with peritrichous flagella	98:215	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium with peritrichous flagella was isolated from a gut sample of the larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
31702533	11	42	theme	protaetiae	1485:1494	arg1	sp					1496:1497	the name Paenibacillus protaetiae sp	1462:1497	the name Paenibacillus protaetiae sp	1462:1497	Hence, strain FW100M-2T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus protaetiae sp.
31702533	2	43	theme	Protaetia	264:272	arg1	larva					255:259	the larva	251:259	the larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea	251:375	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium with peritrichous flagella was isolated from a gut sample of the larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
31702533	8	44	theme	aminophospholipid	1167:1183	arg1	lipids					1069:1074	Polar lipids	1063:1074	Polar lipids of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid, two unidentified phospholipids and an unidentified lipid	1063:1241	Polar lipids of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid, two unidentified phospholipids and an unidentified lipid were present.
31702533	5	45	from	species	927:933	arg1	independent					890:900	independent	890:900	independent	890:900	The phylogenetic tree showed that strain FW100M-2T fell into the genus Paenibacillus, and formed a cluster with P. thailandensis S3-4AT independent from other Paenibacillus species.
31702533	8	46	theme	diphosphatidylglycerol	1079:1100	arg1	lipids					1069:1074	Polar lipids	1063:1074	Polar lipids of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid, two unidentified phospholipids and an unidentified lipid	1063:1241	Polar lipids of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid, two unidentified phospholipids and an unidentified lipid were present.
31702533	4	47	contain	had	687:689	arg1	FW100M-2T					531:539	strain FW100M-2T	524:539	strain FW100M-2T	524:539	16S rRNA gene sequence comparisons indicated that strain FW100M-2T had the highest similarity to type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa.
31702533	4	47	contain	had	687:689	arg2	values					711:716	sequence similarity values	691:716	sequence similarity values less than 96.0 %	691:733	16S rRNA gene sequence comparisons indicated that strain FW100M-2T had the highest similarity to type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa.
31702533	10	48	theme	51.5 mol	1362:1369	arg1	content					1350:1356	The genomic DNA G+C content	1330:1356	The genomic DNA G+C content	1330:1356	The genomic DNA G+C content was 51.5 mol%.
31702533	10	48	theme	51.5 mol	1362:1369	arg1	%					1370:1370	51.5 mol%	1362:1370	51.5 mol%	1362:1370	The genomic DNA G+C content was 51.5 mol%.
31702533	8	49	theme	phosphatidylethanolamine	1103:1126	arg1	lipids					1069:1074	Polar lipids	1063:1074	Polar lipids of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid, two unidentified phospholipids and an unidentified lipid	1063:1241	Polar lipids of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid, two unidentified phospholipids and an unidentified lipid were present.
31702533	8	50	theme	phospholipids	1203:1215	arg1	lipids					1069:1074	Polar lipids	1063:1074	Polar lipids of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid, two unidentified phospholipids and an unidentified lipid	1063:1241	Polar lipids of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid, two unidentified phospholipids and an unidentified lipid were present.
31702533	2	51	theme	gut	237:239	arg1	sample					241:246	a gut sample	235:246	a gut sample of the larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea	235:375	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium with peritrichous flagella was isolated from a gut sample of the larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
31702533	8	52	theme	phosphatidylglycerol	1129:1148	arg1	lipids					1069:1074	Polar lipids	1063:1074	Polar lipids of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid, two unidentified phospholipids and an unidentified lipid	1063:1241	Polar lipids of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid, two unidentified phospholipids and an unidentified lipid were present.
31702533	4	53	theme	thailandensis	601:613	arg1	S3-4AT					615:620	Paenibacillus thailandensis S3-4AT	587:620	Paenibacillus thailandensis S3-4AT (96.8 %)	587:629	16S rRNA gene sequence comparisons indicated that strain FW100M-2T had the highest similarity to type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa.
31702533	4	53	theme	thailandensis	601:613	arg1	%					628:628	96.8 %	623:628	96.8 %	623:628	16S rRNA gene sequence comparisons indicated that strain FW100M-2T had the highest similarity to type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa.
31702533	12	54	theme	=KACC	1534:1538	arg1	113071T					1552:1558	=KACC 19327T=NBRC 113071T	1534:1558	=KACC 19327T=NBRC 113071T	1534:1558	nov. is proposed, with FW100M-2T (=KACC 19327T=NBRC 113071T) as the type strain.
31702533	12	54	theme	=KACC	1534:1538	arg1	FW100M-2T					1523:1531	FW100M-2T	1523:1531	FW100M-2T (=KACC 19327T=NBRC 113071T) as the type strain	1523:1578	nov. is proposed, with FW100M-2T (=KACC 19327T=NBRC 113071T) as the type strain.
31702533	2	55	dep	Protaetia	264:272	arg1	seulensis					286:294	Protaetia brevitarsis seulensis	264:294	Protaetia brevitarsis seulensis	264:294	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium with peritrichous flagella was isolated from a gut sample of the larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
31702533	8	56	theme	lipid	1237:1241	arg1	lipids					1069:1074	Polar lipids	1063:1074	Polar lipids of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid, two unidentified phospholipids and an unidentified lipid	1063:1241	Polar lipids of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid, two unidentified phospholipids and an unidentified lipid were present.
31702533	11	57	theme	Paenibacillus	1437:1449	arg1	genus					1431:1435	the genus Paenibacillus	1427:1449	the genus Paenibacillus	1427:1449	Hence, strain FW100M-2T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus protaetiae sp.
31702533	2	58	theme	larva	255:259	arg1	sample					241:246	a gut sample	235:246	a gut sample of the larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea	235:375	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium with peritrichous flagella was isolated from a gut sample of the larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
31702533	6	59	dep	Antesio-C15 	936:947	arg1	 0					949:950	 0	949:950	 0	949:950	Antesio-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0 were detected as the major fatty acids.
31702533	6	59	dep	Antesio-C15 	936:947	arg1	anteiso-C17 					969:980	anteiso-C17 	969:980	anteiso-C17 	969:980	Antesio-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0 were detected as the major fatty acids.
31702533	6	59	dep	Antesio-C15 	936:947	arg1	 0					962:963	 0	962:963	 0	962:963	Antesio-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0 were detected as the major fatty acids.
31702533	6	59	dep	Antesio-C15 	936:947	arg1	 0					982:983	 0	982:983	 0	982:983	Antesio-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0 were detected as the major fatty acids.
31702533	4	60	theme	agaridevorans	649:661	arg1	1355T					667:671	Paenibacillus agaridevorans DSM 1355T	635:671	Paenibacillus agaridevorans DSM 1355T (96.3 %)	635:680	16S rRNA gene sequence comparisons indicated that strain FW100M-2T had the highest similarity to type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa.
31702533	4	60	theme	agaridevorans	649:661	arg1	%					679:679	96.3 %	674:679	96.3 %	674:679	16S rRNA gene sequence comparisons indicated that strain FW100M-2T had the highest similarity to type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa.
31702533	9	61	from	peptidoglycan	1315:1327	arg1	present					1290:1296	present	1290:1296	present	1290:1296	The meso-diaminopimelic acid was present in the cell-wall peptidoglycan.
31702533	9	61	from	peptidoglycan	1315:1327	arg1	acid					1281:1284	The meso-diaminopimelic acid	1257:1284	The meso-diaminopimelic acid	1257:1284	The meso-diaminopimelic acid was present in the cell-wall peptidoglycan.
31702533	2	62	attach	isolated	221:228	arg2	bacterium					180:188	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium	98:188	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium with peritrichous flagella	98:215	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium with peritrichous flagella was isolated from a gut sample of the larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
31702533	2	62	attach	isolated	221:228	arg1	sample					241:246	a gut sample	235:246	a gut sample of the larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea	235:375	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium with peritrichous flagella was isolated from a gut sample of the larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
31702533	4	63	theme	sequence	691:698	arg1	similarity					700:709	sequence similarity	691:709	sequence similarity values less than 96.0 %	691:733	16S rRNA gene sequence comparisons indicated that strain FW100M-2T had the highest similarity to type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa.
31702533	9	64	theme	meso-diaminopimelic	1261:1279	arg1	present					1290:1296	present	1290:1296	present	1290:1296	The meso-diaminopimelic acid was present in the cell-wall peptidoglycan.
31702533	9	64	theme	meso-diaminopimelic	1261:1279	arg1	acid					1281:1284	The meso-diaminopimelic acid	1257:1284	The meso-diaminopimelic acid	1257:1284	The meso-diaminopimelic acid was present in the cell-wall peptidoglycan.
31702533	1	65	theme	larva	56:60	arg1	gut					49:51	gut	49:51	gut of larva of Protaetia brevitarsis seulensis	49:95	nov., isolated from gut of larva of Protaetia brevitarsis seulensis.
31702533	0	66	theme	protaetiae	14:23	arg1	sp					25:26	Paenibacillus protaetiae sp	0:26	Paenibacillus protaetiae sp.	0:27	Paenibacillus protaetiae sp.
31702533	9	67	from	present	1290:1296	arg1	peptidoglycan					1315:1327	the cell-wall peptidoglycan	1301:1327	the cell-wall peptidoglycan	1301:1327	The meso-diaminopimelic acid was present in the cell-wall peptidoglycan.
31702533	1	68	theme	Protaetia	65:73	arg1	seulensis					87:95	Protaetia brevitarsis seulensis	65:95	Protaetia brevitarsis seulensis	65:95	nov., isolated from gut of larva of Protaetia brevitarsis seulensis.
31702533	2	69	theme	Korea	371:375	arg1	Institute					312:320	the National Institute	299:320	the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea	299:375	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium with peritrichous flagella was isolated from a gut sample of the larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
31702533	1	70	theme	seulensis	87:95	arg1	larva					56:60	larva	56:60	larva of Protaetia brevitarsis seulensis	56:95	nov., isolated from gut of larva of Protaetia brevitarsis seulensis.
31702533	2	71	from	sample	241:246	arg1	Institute					312:320	the National Institute	299:320	the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea	299:375	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium with peritrichous flagella was isolated from a gut sample of the larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
31702533	4	72	theme	strain	524:529	arg1	FW100M-2T					531:539	strain FW100M-2T	524:539	strain FW100M-2T	524:539	16S rRNA gene sequence comparisons indicated that strain FW100M-2T had the highest similarity to type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa.
31702533	9	73	theme	cell-wall	1305:1313	arg1	peptidoglycan					1315:1327	the cell-wall peptidoglycan	1301:1327	the cell-wall peptidoglycan	1301:1327	The meso-diaminopimelic acid was present in the cell-wall peptidoglycan.
31702533	8	74	theme	unidentified	1224:1235	arg1	lipid					1237:1241	an unidentified lipid	1221:1241	an unidentified lipid	1221:1241	Polar lipids of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid, two unidentified phospholipids and an unidentified lipid were present.
31702533	3	75	dep	optimum	411:417	arg1	28-37 °C					420:427	28-37 °C	420:427	28-37 °C	420:427	Growth was observed at 15-50 °C (optimum, 28-37 °C), pH 6.0-8.0 (pH 7.0) and only without NaCl.
31702533	4	76	theme	rRNA	478:481	arg1	comparisons					497:507	16S rRNA gene sequence comparisons	474:507	16S rRNA gene sequence comparisons	474:507	16S rRNA gene sequence comparisons indicated that strain FW100M-2T had the highest similarity to type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa.
31702533	7	77	theme	isoprenoid	1034:1043	arg1	MK-7					1057:1060	MK-7	1057:1060	MK-7	1057:1060	The only isoprenoid quinone was MK-7.
31702533	7	77	theme	isoprenoid	1034:1043	arg1	quinone					1045:1051	The only isoprenoid quinone	1025:1051	The only isoprenoid quinone	1025:1051	The only isoprenoid quinone was MK-7.
31702533	3	78	located	observed	389:396	arg1	pH					431:432	pH 6.0-8.0	431:440	pH 6.0-8.0 (pH 7.0)	431:449	Growth was observed at 15-50 °C (optimum, 28-37 °C), pH 6.0-8.0 (pH 7.0) and only without NaCl.
31702533	3	78	located	observed	389:396	arg1	optimum					411:417	optimum	411:417	optimum	411:417	Growth was observed at 15-50 °C (optimum, 28-37 °C), pH 6.0-8.0 (pH 7.0) and only without NaCl.
31702533	3	78	located	observed	389:396	arg1	15-50 °C					401:408	15-50 °C	401:408	15-50 °C (optimum, 28-37 °C)	401:428	Growth was observed at 15-50 °C (optimum, 28-37 °C), pH 6.0-8.0 (pH 7.0) and only without NaCl.
31702533	3	78	located	observed	389:396	arg2	Growth					378:383	Growth	378:383	Growth	378:383	Growth was observed at 15-50 °C (optimum, 28-37 °C), pH 6.0-8.0 (pH 7.0) and only without NaCl.
31702533	4	79	theme	sequence	488:495	arg1	comparisons					497:507	16S rRNA gene sequence comparisons	474:507	16S rRNA gene sequence comparisons	474:507	16S rRNA gene sequence comparisons indicated that strain FW100M-2T had the highest similarity to type strains of Paenibacillus thailandensis S3-4AT (96.8 %) and Paenibacillus agaridevorans DSM 1355T (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa.
31702533	5	80	theme	Paenibacillus	913:925	arg1	species					927:933	other Paenibacillus species	907:933	other Paenibacillus species	907:933	The phylogenetic tree showed that strain FW100M-2T fell into the genus Paenibacillus, and formed a cluster with P. thailandensis S3-4AT independent from other Paenibacillus species.
31702533	2	81	with	bacterium	180:188	arg1	flagella					208:215	peritrichous flagella	195:215	peritrichous flagella	195:215	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium with peritrichous flagella was isolated from a gut sample of the larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
31702533	2	82	theme	peritrichous	195:206	arg1	flagella					208:215	peritrichous flagella	195:215	peritrichous flagella	195:215	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium with peritrichous flagella was isolated from a gut sample of the larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
31702533	10	83	theme	genomic	1334:1340	arg1	content					1350:1356	The genomic DNA G+C content	1330:1356	The genomic DNA G+C content	1330:1356	The genomic DNA G+C content was 51.5 mol%.
31702533	10	83	theme	genomic	1334:1340	arg1	%					1370:1370	51.5 mol%	1362:1370	51.5 mol%	1362:1370	The genomic DNA G+C content was 51.5 mol%.
31702533	6	84	theme	fatty	1012:1016	arg1	Antesio-C15 					936:947	Antesio-C15 	936:947	Antesio-C15 	936:947	Antesio-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0 were detected as the major fatty acids.
31702533	6	84	theme	fatty	1012:1016	arg1	acids					1018:1022	the major fatty acids	1002:1022	the major fatty acids	1002:1022	Antesio-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0 were detected as the major fatty acids.
31702533	2	85	theme	rod-shaped	121:130	arg1	bacterium					180:188	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium	98:188	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium with peritrichous flagella	98:215	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium with peritrichous flagella was isolated from a gut sample of the larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
31702533	2	86	theme	Republic	359:366	arg1	Institute					312:320	the National Institute	299:320	the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea	299:375	A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium with peritrichous flagella was isolated from a gut sample of the larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea.
32537020	0	0	theme	polysaccharides	103:117	arg1	activity					134:141	anti-tumor activity	123:141	anti-tumor activity	123:141	Sulfated modification, characterization and monosaccharide composition analysis of Undaria pinnatifida polysaccharides and anti-tumor activity.
32537020	0	0	theme	polysaccharides	103:117	arg1	analysis					71:78	modification, characterization and monosaccharide composition analysis	9:78	modification, characterization and monosaccharide composition analysis of Undaria pinnatifida polysaccharides	9:117	Sulfated modification, characterization and monosaccharide composition analysis of Undaria pinnatifida polysaccharides and anti-tumor activity.
32537020	8	1	theme	radical	1082:1088	arg1	content					1090:1096	The sulfate radical content	1070:1096	The sulfate radical content of UPPS-B1 and S-UPPS-B1	1070:1121	The sulfate radical content of UPPS-B1 and S-UPPS-B1 was 8.53 and 29.12%, whilst the content of uronic acid was 9.29 and 7.98%, respectively.
32537020	8	1	theme	radical	1082:1088	arg1	%					1141:1141	8.53 and 29.12%	1127:1141	8.53 and 29.12%	1127:1141	The sulfate radical content of UPPS-B1 and S-UPPS-B1 was 8.53 and 29.12%, whilst the content of uronic acid was 9.29 and 7.98%, respectively.
32537020	6	2	theme	high-performance	945:960	arg1	chromatography					969:982	high-performance liquid chromatography	945:982	high-performance liquid chromatography	945:982	UPPS-B1 and sulfated (S)-UPPS-B1 were characterized via chemical analysis, ultraviolet-visible and Fourier-transformed infrared spectroscopy, gas chromatography and high-performance liquid chromatography.
32537020	1	3	theme	pinnatifida	262:272	arg1	polysaccharides					181:195	Undaria pinnatifida (U. pinnatifida) polysaccharides	144:195	Undaria pinnatifida (U. pinnatifida) polysaccharides (UPPS)	144:202	Undaria pinnatifida (U. pinnatifida) polysaccharides (UPPS) are considered to be the major bioactive components of U. pinnatifida.
32537020	1	3	theme	pinnatifida	262:272	arg1	components					245:254	the major bioactive components	225:254	the major bioactive components of U. pinnatifida	225:272	Undaria pinnatifida (U. pinnatifida) polysaccharides (UPPS) are considered to be the major bioactive components of U. pinnatifida.
32537020	0	4	theme	pinnatifida	91:101	arg1	polysaccharides					103:117	Undaria pinnatifida polysaccharides	83:117	Undaria pinnatifida polysaccharides	83:117	Sulfated modification, characterization and monosaccharide composition analysis of Undaria pinnatifida polysaccharides and anti-tumor activity.
32537020	8	5	theme	sulfate	1074:1080	arg1	content					1090:1096	The sulfate radical content	1070:1096	The sulfate radical content of UPPS-B1 and S-UPPS-B1	1070:1121	The sulfate radical content of UPPS-B1 and S-UPPS-B1 was 8.53 and 29.12%, whilst the content of uronic acid was 9.29 and 7.98%, respectively.
32537020	8	5	theme	sulfate	1074:1080	arg1	%					1141:1141	8.53 and 29.12%	1127:1141	8.53 and 29.12%	1127:1141	The sulfate radical content of UPPS-B1 and S-UPPS-B1 was 8.53 and 29.12%, whilst the content of uronic acid was 9.29 and 7.98%, respectively.
32537020	6	6	theme	chemical	836:843	arg1	analysis					845:852	chemical analysis	836:852	chemical analysis	836:852	UPPS-B1 and sulfated (S)-UPPS-B1 were characterized via chemical analysis, ultraviolet-visible and Fourier-transformed infrared spectroscopy, gas chromatography and high-performance liquid chromatography.
32537020	6	7	dep	sulfated	792:799	arg1	S					802:802	S	802:802	S	802:802	UPPS-B1 and sulfated (S)-UPPS-B1 were characterized via chemical analysis, ultraviolet-visible and Fourier-transformed infrared spectroscopy, gas chromatography and high-performance liquid chromatography.
32537020	4	8	theme	major	575:579	arg1	fraction					596:603	The major polysaccharide fraction	571:603	The major polysaccharide fraction of U. pinnatifida (UPPS-B1)	571:631	The major polysaccharide fraction of U. pinnatifida (UPPS-B1) was purified via DEAE-52 and Sephadex G-200 column chromatography.
32537020	0	9	theme	anti-tumor	123:132	arg1	activity					134:141	anti-tumor activity	123:141	anti-tumor activity	123:141	Sulfated modification, characterization and monosaccharide composition analysis of Undaria pinnatifida polysaccharides and anti-tumor activity.
32537020	6	10	theme	liquid	962:967	arg1	chromatography					969:982	high-performance liquid chromatography	945:982	high-performance liquid chromatography	945:982	UPPS-B1 and sulfated (S)-UPPS-B1 were characterized via chemical analysis, ultraviolet-visible and Fourier-transformed infrared spectroscopy, gas chromatography and high-performance liquid chromatography.
32537020	2	11	theme	study	298:302	arg1	aim					279:281	The aim	275:281	The aim of the present study	275:302	The aim of the present study was to investigate the separation, sulfated modification, characterization and monosaccharide composition of UPPS.
32537020	11	12	theme	1.0:9.7:6.4:1.6	1566:1580	arg1	ratio					1557:1561	a ratio	1555:1561	a ratio of 1.0:9.7:6.4:1.6	1555:1580	In addition, S-UPPS-B1 was a heteropolysaccharide composed of xylose, mannose, glucose and galactose at a ratio of 1.0:9.7:6.4:1.6.
32537020	5	13	theme	acid-pyridine	719:731	arg1	method					733:738	The chlorosulfonic acid-pyridine method	700:738	The chlorosulfonic acid-pyridine method	700:738	The chlorosulfonic acid-pyridine method was applied for sulfation modification.
32537020	7	14	theme	UPPS-B1	1012:1018	arg1	content					1001:1007	The total sugar content	985:1007	The total sugar content of UPPS-B1 and S-UPPS-B1	985:1032	The total sugar content of UPPS-B1 and S-UPPS-B1 was 79.78 and 77.28%, respectively.
32537020	7	14	theme	UPPS-B1	1012:1018	arg1	%					1053:1053	79.78 and 77.28%	1038:1053	79.78 and 77.28%	1038:1053	The total sugar content of UPPS-B1 and S-UPPS-B1 was 79.78 and 77.28%, respectively.
32537020	2	15	theme	present	290:296	arg1	study					298:302	the present study	286:302	the present study	286:302	The aim of the present study was to investigate the separation, sulfated modification, characterization and monosaccharide composition of UPPS.
32537020	5	16	theme	sulfation	756:764	arg1	modification					766:777	sulfation modification	756:777	sulfation modification	756:777	The chlorosulfonic acid-pyridine method was applied for sulfation modification.
32537020	10	17	theme	7.9:8.7:12.0:9.8	1433:1448	arg1	ratio					1424:1428	a ratio	1422:1428	a ratio of 7.9:8.7:12.0:9.8	1422:1448	UPPS-B1 was considered to be a heteropolysaccharide composed of xylose, mannose, glucose and galactose at a ratio of 7.9:8.7:12.0:9.8.
32537020	3	18	theme	optimal	423:429	arg1	conditions					442:451	The optimal processing conditions	419:451	The optimal processing conditions	419:451	The optimal processing conditions were as follows: Distilled water-to-solid ratio, 50 ml/g; extraction time, 300 min; and extraction temperature, 90˚C.
32537020	3	19	theme	processing	431:440	arg1	conditions					442:451	The optimal processing conditions	419:451	The optimal processing conditions	419:451	The optimal processing conditions were as follows: Distilled water-to-solid ratio, 50 ml/g; extraction time, 300 min; and extraction temperature, 90˚C.
32537020	9	20	theme	average	1216:1222	arg1	kD					1298:1299	37 and 110 kD	1287:1299	37 and 110 kD	1287:1299	The average molecular weight of UPPS-B1 and S-UPPS-B1 was determined to be 37 and 110 kD, respectively.
32537020	9	20	theme	average	1216:1222	arg1	weight					1234:1239	The average molecular weight	1212:1239	The average molecular weight of UPPS-B1 and S-UPPS-B1	1212:1264	The average molecular weight of UPPS-B1 and S-UPPS-B1 was determined to be 37 and 110 kD, respectively.
32537020	9	21	theme	S-UPPS-B1	1256:1264	arg1	kD					1298:1299	37 and 110 kD	1287:1299	37 and 110 kD	1287:1299	The average molecular weight of UPPS-B1 and S-UPPS-B1 was determined to be 37 and 110 kD, respectively.
32537020	9	21	theme	S-UPPS-B1	1256:1264	arg1	weight					1234:1239	The average molecular weight	1212:1239	The average molecular weight of UPPS-B1 and S-UPPS-B1	1212:1264	The average molecular weight of UPPS-B1 and S-UPPS-B1 was determined to be 37 and 110 kD, respectively.
32537020	2	22	theme	UPPS	413:416	arg1	characterization					362:377	characterization	362:377	characterization	362:377	The aim of the present study was to investigate the separation, sulfated modification, characterization and monosaccharide composition of UPPS.
32537020	2	22	theme	UPPS	413:416	arg1	separation					327:336	separation	327:336	separation	327:336	The aim of the present study was to investigate the separation, sulfated modification, characterization and monosaccharide composition of UPPS.
32537020	2	22	theme	UPPS	413:416	arg1	modification					348:359	sulfated modification	339:359	sulfated modification	339:359	The aim of the present study was to investigate the separation, sulfated modification, characterization and monosaccharide composition of UPPS.
32537020	2	22	theme	UPPS	413:416	arg1	composition					398:408	monosaccharide composition	383:408	monosaccharide composition	383:408	The aim of the present study was to investigate the separation, sulfated modification, characterization and monosaccharide composition of UPPS.
32537020	0	23	theme	modification	9:20	arg1	analysis					71:78	modification, characterization and monosaccharide composition analysis	9:78	modification, characterization and monosaccharide composition analysis of Undaria pinnatifida polysaccharides	9:117	Sulfated modification, characterization and monosaccharide composition analysis of Undaria pinnatifida polysaccharides and anti-tumor activity.
32537020	6	24	theme	gas	922:924	arg1	chromatography					926:939	gas chromatography	922:939	gas chromatography	922:939	UPPS-B1 and sulfated (S)-UPPS-B1 were characterized via chemical analysis, ultraviolet-visible and Fourier-transformed infrared spectroscopy, gas chromatography and high-performance liquid chromatography.
32537020	8	25	theme	uronic	1166:1171	arg1	acid					1173:1176	uronic acid	1166:1176	uronic acid	1166:1176	The sulfate radical content of UPPS-B1 and S-UPPS-B1 was 8.53 and 29.12%, whilst the content of uronic acid was 9.29 and 7.98%, respectively.
32537020	12	26	theme	anti-tumor	1673:1682	arg1	activity					1684:1691	anti-tumor activity	1673:1691	anti-tumor activity	1673:1691	The results of the tumor growth inhibition experiment demonstrated that UPPS-B1 exhibited anti-tumor activity in vivo, which was improved following sulfation to yield S-UPPS-B1.
32537020	4	27	theme	G-200	671:675	arg1	chromatography					684:697	Sephadex G-200 column chromatography	662:697	Sephadex G-200 column chromatography	662:697	The major polysaccharide fraction of U. pinnatifida (UPPS-B1) was purified via DEAE-52 and Sephadex G-200 column chromatography.
32537020	8	28	theme	S-UPPS-B1	1113:1121	arg1	content					1090:1096	The sulfate radical content	1070:1096	The sulfate radical content of UPPS-B1 and S-UPPS-B1	1070:1121	The sulfate radical content of UPPS-B1 and S-UPPS-B1 was 8.53 and 29.12%, whilst the content of uronic acid was 9.29 and 7.98%, respectively.
32537020	8	28	theme	S-UPPS-B1	1113:1121	arg1	%					1141:1141	8.53 and 29.12%	1127:1141	8.53 and 29.12%	1127:1141	The sulfate radical content of UPPS-B1 and S-UPPS-B1 was 8.53 and 29.12%, whilst the content of uronic acid was 9.29 and 7.98%, respectively.
32537020	7	29	theme	total	989:993	arg1	content					1001:1007	The total sugar content	985:1007	The total sugar content of UPPS-B1 and S-UPPS-B1	985:1032	The total sugar content of UPPS-B1 and S-UPPS-B1 was 79.78 and 77.28%, respectively.
32537020	7	29	theme	total	989:993	arg1	%					1053:1053	79.78 and 77.28%	1038:1053	79.78 and 77.28%	1038:1053	The total sugar content of UPPS-B1 and S-UPPS-B1 was 79.78 and 77.28%, respectively.
32537020	2	30	dep	separation	327:336	arg1	the					323:325	the	323:325	the	323:325	The aim of the present study was to investigate the separation, sulfated modification, characterization and monosaccharide composition of UPPS.
32537020	12	31	theme	experiment	1626:1635	arg1	results					1587:1593	The results	1583:1593	The results of the tumor growth inhibition experiment	1583:1635	The results of the tumor growth inhibition experiment demonstrated that UPPS-B1 exhibited anti-tumor activity in vivo, which was improved following sulfation to yield S-UPPS-B1.
32537020	4	32	theme	Sephadex	662:669	arg1	chromatography					684:697	Sephadex G-200 column chromatography	662:697	Sephadex G-200 column chromatography	662:697	The major polysaccharide fraction of U. pinnatifida (UPPS-B1) was purified via DEAE-52 and Sephadex G-200 column chromatography.
32537020	0	33	theme	characterization	23:38	arg1	analysis					71:78	modification, characterization and monosaccharide composition analysis	9:78	modification, characterization and monosaccharide composition analysis of Undaria pinnatifida polysaccharides	9:117	Sulfated modification, characterization and monosaccharide composition analysis of Undaria pinnatifida polysaccharides and anti-tumor activity.
32537020	10	34	from	ratio	1424:1428	arg1	galactose					1409:1417	galactose	1409:1417	galactose	1409:1417	UPPS-B1 was considered to be a heteropolysaccharide composed of xylose, mannose, glucose and galactose at a ratio of 7.9:8.7:12.0:9.8.
32537020	10	34	from	ratio	1424:1428	arg1	glucose					1397:1403	glucose	1397:1403	glucose	1397:1403	UPPS-B1 was considered to be a heteropolysaccharide composed of xylose, mannose, glucose and galactose at a ratio of 7.9:8.7:12.0:9.8.
32537020	10	34	from	ratio	1424:1428	arg1	mannose					1388:1394	mannose	1388:1394	mannose	1388:1394	UPPS-B1 was considered to be a heteropolysaccharide composed of xylose, mannose, glucose and galactose at a ratio of 7.9:8.7:12.0:9.8.
32537020	10	34	from	ratio	1424:1428	arg1	heteropolysaccharide					1347:1366	a heteropolysaccharide	1345:1366	a heteropolysaccharide composed of xylose	1345:1385	UPPS-B1 was considered to be a heteropolysaccharide composed of xylose, mannose, glucose and galactose at a ratio of 7.9:8.7:12.0:9.8.
32537020	5	35	theme	chlorosulfonic	704:717	arg1	method					733:738	The chlorosulfonic acid-pyridine method	700:738	The chlorosulfonic acid-pyridine method	700:738	The chlorosulfonic acid-pyridine method was applied for sulfation modification.
32537020	7	36	theme	sugar	995:999	arg1	content					1001:1007	The total sugar content	985:1007	The total sugar content of UPPS-B1 and S-UPPS-B1	985:1032	The total sugar content of UPPS-B1 and S-UPPS-B1 was 79.78 and 77.28%, respectively.
32537020	7	36	theme	sugar	995:999	arg1	%					1053:1053	79.78 and 77.28%	1038:1053	79.78 and 77.28%	1038:1053	The total sugar content of UPPS-B1 and S-UPPS-B1 was 79.78 and 77.28%, respectively.
32537020	11	37	from	heteropolysaccharide	1480:1499	arg1	addition					1454:1461	addition	1454:1461	addition	1454:1461	In addition, S-UPPS-B1 was a heteropolysaccharide composed of xylose, mannose, glucose and galactose at a ratio of 1.0:9.7:6.4:1.6.
32537020	7	38	theme	S-UPPS-B1	1024:1032	arg1	content					1001:1007	The total sugar content	985:1007	The total sugar content of UPPS-B1 and S-UPPS-B1	985:1032	The total sugar content of UPPS-B1 and S-UPPS-B1 was 79.78 and 77.28%, respectively.
32537020	7	38	theme	S-UPPS-B1	1024:1032	arg1	%					1053:1053	79.78 and 77.28%	1038:1053	79.78 and 77.28%	1038:1053	The total sugar content of UPPS-B1 and S-UPPS-B1 was 79.78 and 77.28%, respectively.
32537020	12	39	theme	growth	1608:1613	arg1	inhibition					1615:1624	tumor growth inhibition	1602:1624	the tumor growth inhibition experiment	1598:1635	The results of the tumor growth inhibition experiment demonstrated that UPPS-B1 exhibited anti-tumor activity in vivo, which was improved following sulfation to yield S-UPPS-B1.
32537020	4	40	theme	pinnatifida	611:621	arg1	fraction					596:603	The major polysaccharide fraction	571:603	The major polysaccharide fraction of U. pinnatifida (UPPS-B1)	571:631	The major polysaccharide fraction of U. pinnatifida (UPPS-B1) was purified via DEAE-52 and Sephadex G-200 column chromatography.
32537020	9	41	theme	UPPS-B1	1244:1250	arg1	kD					1298:1299	37 and 110 kD	1287:1299	37 and 110 kD	1287:1299	The average molecular weight of UPPS-B1 and S-UPPS-B1 was determined to be 37 and 110 kD, respectively.
32537020	9	41	theme	UPPS-B1	1244:1250	arg1	weight					1234:1239	The average molecular weight	1212:1239	The average molecular weight of UPPS-B1 and S-UPPS-B1	1212:1264	The average molecular weight of UPPS-B1 and S-UPPS-B1 was determined to be 37 and 110 kD, respectively.
32537020	3	42	theme	extraction	511:520	arg1	ratio					495:499	Distilled water-to-solid ratio	470:499	Distilled water-to-solid ratio	470:499	The optimal processing conditions were as follows: Distilled water-to-solid ratio, 50 ml/g; extraction time, 300 min; and extraction temperature, 90˚C.
32537020	3	42	theme	extraction	511:520	arg1	time					522:525	50 ml/g; extraction time	502:525	50 ml/g; extraction time	502:525	The optimal processing conditions were as follows: Distilled water-to-solid ratio, 50 ml/g; extraction time, 300 min; and extraction temperature, 90˚C.
32537020	8	43	theme	acid	1173:1176	arg1	%					1195:1195	9.29 and 7.98%	1182:1195	9.29 and 7.98%	1182:1195	The sulfate radical content of UPPS-B1 and S-UPPS-B1 was 8.53 and 29.12%, whilst the content of uronic acid was 9.29 and 7.98%, respectively.
32537020	8	43	theme	acid	1173:1176	arg1	content					1155:1161	the content	1151:1161	the content of uronic acid	1151:1176	The sulfate radical content of UPPS-B1 and S-UPPS-B1 was 8.53 and 29.12%, whilst the content of uronic acid was 9.29 and 7.98%, respectively.
32537020	0	44	theme	monosaccharide	44:57	arg1	composition					59:69	monosaccharide composition	44:69	monosaccharide composition	44:69	Sulfated modification, characterization and monosaccharide composition analysis of Undaria pinnatifida polysaccharides and anti-tumor activity.
32537020	2	45	theme	sulfated	339:346	arg1	modification					348:359	sulfated modification	339:359	sulfated modification	339:359	The aim of the present study was to investigate the separation, sulfated modification, characterization and monosaccharide composition of UPPS.
32537020	12	46	theme	tumor	1602:1606	arg1	inhibition					1615:1624	tumor growth inhibition	1602:1624	the tumor growth inhibition experiment	1598:1635	The results of the tumor growth inhibition experiment demonstrated that UPPS-B1 exhibited anti-tumor activity in vivo, which was improved following sulfation to yield S-UPPS-B1.
32537020	3	47	theme	Distilled	470:478	arg1	90˚C					565:568	90˚C	565:568	90˚C	565:568	The optimal processing conditions were as follows: Distilled water-to-solid ratio, 50 ml/g; extraction time, 300 min; and extraction temperature, 90˚C.
32537020	3	47	theme	Distilled	470:478	arg1	temperature					552:562	extraction temperature	541:562	extraction temperature	541:562	The optimal processing conditions were as follows: Distilled water-to-solid ratio, 50 ml/g; extraction time, 300 min; and extraction temperature, 90˚C.
32537020	3	47	theme	Distilled	470:478	arg1	ratio					495:499	Distilled water-to-solid ratio	470:499	Distilled water-to-solid ratio	470:499	The optimal processing conditions were as follows: Distilled water-to-solid ratio, 50 ml/g; extraction time, 300 min; and extraction temperature, 90˚C.
32537020	3	47	theme	Distilled	470:478	arg1	time					522:525	50 ml/g; extraction time	502:525	50 ml/g; extraction time	502:525	The optimal processing conditions were as follows: Distilled water-to-solid ratio, 50 ml/g; extraction time, 300 min; and extraction temperature, 90˚C.
32537020	3	47	theme	Distilled	470:478	arg1	min					532:534	300 min	528:534	300 min	528:534	The optimal processing conditions were as follows: Distilled water-to-solid ratio, 50 ml/g; extraction time, 300 min; and extraction temperature, 90˚C.
32537020	6	48	theme	infrared	899:906	arg1	spectroscopy					908:919	Fourier-transformed infrared spectroscopy	879:919	Fourier-transformed infrared spectroscopy	879:919	UPPS-B1 and sulfated (S)-UPPS-B1 were characterized via chemical analysis, ultraviolet-visible and Fourier-transformed infrared spectroscopy, gas chromatography and high-performance liquid chromatography.
32537020	6	49	theme	sulfated	792:799	arg1	-UPPS-B1					804:811	UPPS-B1 and sulfated (S)-UPPS-B1	780:811	UPPS-B1 and sulfated (S)-UPPS-B1	780:811	UPPS-B1 and sulfated (S)-UPPS-B1 were characterized via chemical analysis, ultraviolet-visible and Fourier-transformed infrared spectroscopy, gas chromatography and high-performance liquid chromatography.
32537020	3	50	theme	water-to-solid	480:493	arg1	90˚C					565:568	90˚C	565:568	90˚C	565:568	The optimal processing conditions were as follows: Distilled water-to-solid ratio, 50 ml/g; extraction time, 300 min; and extraction temperature, 90˚C.
32537020	3	50	theme	water-to-solid	480:493	arg1	temperature					552:562	extraction temperature	541:562	extraction temperature	541:562	The optimal processing conditions were as follows: Distilled water-to-solid ratio, 50 ml/g; extraction time, 300 min; and extraction temperature, 90˚C.
32537020	3	50	theme	water-to-solid	480:493	arg1	ratio					495:499	Distilled water-to-solid ratio	470:499	Distilled water-to-solid ratio	470:499	The optimal processing conditions were as follows: Distilled water-to-solid ratio, 50 ml/g; extraction time, 300 min; and extraction temperature, 90˚C.
32537020	3	50	theme	water-to-solid	480:493	arg1	time					522:525	50 ml/g; extraction time	502:525	50 ml/g; extraction time	502:525	The optimal processing conditions were as follows: Distilled water-to-solid ratio, 50 ml/g; extraction time, 300 min; and extraction temperature, 90˚C.
32537020	3	50	theme	water-to-solid	480:493	arg1	min					532:534	300 min	528:534	300 min	528:534	The optimal processing conditions were as follows: Distilled water-to-solid ratio, 50 ml/g; extraction time, 300 min; and extraction temperature, 90˚C.
32537020	6	51	theme	Fourier-transformed	879:897	arg1	spectroscopy					908:919	Fourier-transformed infrared spectroscopy	879:919	Fourier-transformed infrared spectroscopy	879:919	UPPS-B1 and sulfated (S)-UPPS-B1 were characterized via chemical analysis, ultraviolet-visible and Fourier-transformed infrared spectroscopy, gas chromatography and high-performance liquid chromatography.
32537020	1	52	theme	Undaria	144:150	arg1	pinnatifida					152:162	Undaria pinnatifida	144:162	Undaria pinnatifida (U. pinnatifida) polysaccharides (UPPS)	144:202	Undaria pinnatifida (U. pinnatifida) polysaccharides (UPPS) are considered to be the major bioactive components of U. pinnatifida.
32537020	1	52	theme	Undaria	144:150	arg1	pinnatifida					168:178	pinnatifida	168:178	pinnatifida	168:178	Undaria pinnatifida (U. pinnatifida) polysaccharides (UPPS) are considered to be the major bioactive components of U. pinnatifida.
32537020	0	53	theme	composition	59:69	arg1	analysis					71:78	modification, characterization and monosaccharide composition analysis	9:78	modification, characterization and monosaccharide composition analysis of Undaria pinnatifida polysaccharides	9:117	Sulfated modification, characterization and monosaccharide composition analysis of Undaria pinnatifida polysaccharides and anti-tumor activity.
32537020	8	54	theme	UPPS-B1	1101:1107	arg1	content					1090:1096	The sulfate radical content	1070:1096	The sulfate radical content of UPPS-B1 and S-UPPS-B1	1070:1121	The sulfate radical content of UPPS-B1 and S-UPPS-B1 was 8.53 and 29.12%, whilst the content of uronic acid was 9.29 and 7.98%, respectively.
32537020	8	54	theme	UPPS-B1	1101:1107	arg1	%					1141:1141	8.53 and 29.12%	1127:1141	8.53 and 29.12%	1127:1141	The sulfate radical content of UPPS-B1 and S-UPPS-B1 was 8.53 and 29.12%, whilst the content of uronic acid was 9.29 and 7.98%, respectively.
32537020	6	55	theme	UPPS-B1	780:786	arg1	-UPPS-B1					804:811	UPPS-B1 and sulfated (S)-UPPS-B1	780:811	UPPS-B1 and sulfated (S)-UPPS-B1	780:811	UPPS-B1 and sulfated (S)-UPPS-B1 were characterized via chemical analysis, ultraviolet-visible and Fourier-transformed infrared spectroscopy, gas chromatography and high-performance liquid chromatography.
32537020	1	56	theme	pinnatifida	152:162	arg1	UPPS					198:201	UPPS	198:201	UPPS	198:201	Undaria pinnatifida (U. pinnatifida) polysaccharides (UPPS) are considered to be the major bioactive components of U. pinnatifida.
32537020	1	56	theme	pinnatifida	152:162	arg1	polysaccharides					181:195	Undaria pinnatifida (U. pinnatifida) polysaccharides	144:195	Undaria pinnatifida (U. pinnatifida) polysaccharides (UPPS)	144:202	Undaria pinnatifida (U. pinnatifida) polysaccharides (UPPS) are considered to be the major bioactive components of U. pinnatifida.
32537020	1	56	theme	pinnatifida	152:162	arg1	components					245:254	the major bioactive components	225:254	the major bioactive components of U. pinnatifida	225:272	Undaria pinnatifida (U. pinnatifida) polysaccharides (UPPS) are considered to be the major bioactive components of U. pinnatifida.
32537020	12	57	theme	inhibition	1615:1624	arg1	experiment					1626:1635	the tumor growth inhibition experiment	1598:1635	the tumor growth inhibition experiment	1598:1635	The results of the tumor growth inhibition experiment demonstrated that UPPS-B1 exhibited anti-tumor activity in vivo, which was improved following sulfation to yield S-UPPS-B1.
32537020	11	58	from	ratio	1557:1561	arg1	heteropolysaccharide					1480:1499	a heteropolysaccharide	1478:1499	a heteropolysaccharide composed of xylose	1478:1518	In addition, S-UPPS-B1 was a heteropolysaccharide composed of xylose, mannose, glucose and galactose at a ratio of 1.0:9.7:6.4:1.6.
32537020	11	58	from	ratio	1557:1561	arg1	galactose					1542:1550	galactose	1542:1550	galactose	1542:1550	In addition, S-UPPS-B1 was a heteropolysaccharide composed of xylose, mannose, glucose and galactose at a ratio of 1.0:9.7:6.4:1.6.
32537020	11	58	from	ratio	1557:1561	arg1	glucose					1530:1536	glucose	1530:1536	glucose	1530:1536	In addition, S-UPPS-B1 was a heteropolysaccharide composed of xylose, mannose, glucose and galactose at a ratio of 1.0:9.7:6.4:1.6.
32537020	11	58	from	ratio	1557:1561	arg1	mannose					1521:1527	mannose	1521:1527	mannose	1521:1527	In addition, S-UPPS-B1 was a heteropolysaccharide composed of xylose, mannose, glucose and galactose at a ratio of 1.0:9.7:6.4:1.6.
32537020	1	59	theme	bioactive	235:243	arg1	polysaccharides					181:195	Undaria pinnatifida (U. pinnatifida) polysaccharides	144:195	Undaria pinnatifida (U. pinnatifida) polysaccharides (UPPS)	144:202	Undaria pinnatifida (U. pinnatifida) polysaccharides (UPPS) are considered to be the major bioactive components of U. pinnatifida.
32537020	1	59	theme	bioactive	235:243	arg1	components					245:254	the major bioactive components	225:254	the major bioactive components of U. pinnatifida	225:272	Undaria pinnatifida (U. pinnatifida) polysaccharides (UPPS) are considered to be the major bioactive components of U. pinnatifida.
32537020	3	60	theme	extraction	541:550	arg1	temperature					552:562	extraction temperature	541:562	extraction temperature	541:562	The optimal processing conditions were as follows: Distilled water-to-solid ratio, 50 ml/g; extraction time, 300 min; and extraction temperature, 90˚C.
32537020	3	60	theme	extraction	541:550	arg1	ratio					495:499	Distilled water-to-solid ratio	470:499	Distilled water-to-solid ratio	470:499	The optimal processing conditions were as follows: Distilled water-to-solid ratio, 50 ml/g; extraction time, 300 min; and extraction temperature, 90˚C.
32537020	4	61	theme	polysaccharide	581:594	arg1	fraction					596:603	The major polysaccharide fraction	571:603	The major polysaccharide fraction of U. pinnatifida (UPPS-B1)	571:631	The major polysaccharide fraction of U. pinnatifida (UPPS-B1) was purified via DEAE-52 and Sephadex G-200 column chromatography.
32537020	3	62	theme	ml/g	505:508	arg1	ratio					495:499	Distilled water-to-solid ratio	470:499	Distilled water-to-solid ratio	470:499	The optimal processing conditions were as follows: Distilled water-to-solid ratio, 50 ml/g; extraction time, 300 min; and extraction temperature, 90˚C.
32537020	3	62	theme	ml/g	505:508	arg1	time					522:525	50 ml/g; extraction time	502:525	50 ml/g; extraction time	502:525	The optimal processing conditions were as follows: Distilled water-to-solid ratio, 50 ml/g; extraction time, 300 min; and extraction temperature, 90˚C.
32537020	0	63	theme	Undaria	83:89	arg1	polysaccharides					103:117	Undaria pinnatifida polysaccharides	83:117	Undaria pinnatifida polysaccharides	83:117	Sulfated modification, characterization and monosaccharide composition analysis of Undaria pinnatifida polysaccharides and anti-tumor activity.
32537020	1	64	theme	major	229:233	arg1	polysaccharides					181:195	Undaria pinnatifida (U. pinnatifida) polysaccharides	144:195	Undaria pinnatifida (U. pinnatifida) polysaccharides (UPPS)	144:202	Undaria pinnatifida (U. pinnatifida) polysaccharides (UPPS) are considered to be the major bioactive components of U. pinnatifida.
32537020	1	64	theme	major	229:233	arg1	components					245:254	the major bioactive components	225:254	the major bioactive components of U. pinnatifida	225:272	Undaria pinnatifida (U. pinnatifida) polysaccharides (UPPS) are considered to be the major bioactive components of U. pinnatifida.
32537020	2	65	theme	monosaccharide	383:396	arg1	composition					398:408	monosaccharide composition	383:408	monosaccharide composition	383:408	The aim of the present study was to investigate the separation, sulfated modification, characterization and monosaccharide composition of UPPS.
32537020	9	66	theme	molecular	1224:1232	arg1	kD					1298:1299	37 and 110 kD	1287:1299	37 and 110 kD	1287:1299	The average molecular weight of UPPS-B1 and S-UPPS-B1 was determined to be 37 and 110 kD, respectively.
32537020	9	66	theme	molecular	1224:1232	arg1	weight					1234:1239	The average molecular weight	1212:1239	The average molecular weight of UPPS-B1 and S-UPPS-B1	1212:1264	The average molecular weight of UPPS-B1 and S-UPPS-B1 was determined to be 37 and 110 kD, respectively.
32537020	12	67	dep	exhibited	1663:1671	arg1	improved					1712:1719	improved	1712:1719	was improved following sulfation to yield S-UPPS-B1	1708:1758	The results of the tumor growth inhibition experiment demonstrated that UPPS-B1 exhibited anti-tumor activity in vivo, which was improved following sulfation to yield S-UPPS-B1.
32537020	4	68	theme	column	677:682	arg1	chromatography					684:697	Sephadex G-200 column chromatography	662:697	Sephadex G-200 column chromatography	662:697	The major polysaccharide fraction of U. pinnatifida (UPPS-B1) was purified via DEAE-52 and Sephadex G-200 column chromatography.
32537020	1	69	theme	U.	259:260	arg1	pinnatifida					262:272	U. pinnatifida	259:272	U. pinnatifida	259:272	Undaria pinnatifida (U. pinnatifida) polysaccharides (UPPS) are considered to be the major bioactive components of U. pinnatifida.
32696223	5	0	theme	cell	716:719	arg1	models					729:734	cell culture models	716:734	cell culture models	716:734	In this study, we investigated the relationship between HIF-1α and autophagy in healthy and inflammatory states using transgenic mice, colitis models, and cell culture models.
32696223	7	1	theme	HIF-1α∆IEC	1073:1082	arg1	mice					1085:1088	IEC-specific HIF-1α-deficient (HIF-1α∆IEC) mice	1042:1088	IEC-specific HIF-1α-deficient (HIF-1α∆IEC) mice	1042:1088	In addition, autophagy levels in the intestinal epithelial cells (IECs) were significantly reduced in IEC-specific HIF-1α-deficient (HIF-1α∆IEC) mice.
32696223	10	2	theme	butyrate-mediated	1472:1488	arg1	protection					1490:1499	the butyrate-mediated protection	1468:1499	the butyrate-mediated protection against colonic injury	1468:1522	However, the butyrate-mediated protection against colonic injury was considerably diminished in the HIF-1α∆IEC mice.
32696223	10	3	theme	colonic	1509:1515	arg1	injury					1517:1522	colonic injury	1509:1522	colonic injury	1509:1522	However, the butyrate-mediated protection against colonic injury was considerably diminished in the HIF-1α∆IEC mice.
32696223	5	4	theme	culture	721:727	arg1	models					729:734	cell culture models	716:734	cell culture models	716:734	In this study, we investigated the relationship between HIF-1α and autophagy in healthy and inflammatory states using transgenic mice, colitis models, and cell culture models.
32696223	8	5	theme	HIF-1α	1275:1280	arg1	ablation					1282:1289	HIF-1α ablation	1275:1289	HIF-1α ablation	1275:1289	Moreover, in the cell culture models, butyrate treatment significantly increased autophagy in HT29 cells under normal conditions, whereas butyrate had little effect on autophagy after HIF-1α ablation.
32696223	14	6	theme	intestinal	1916:1925	arg1	HIF-1α					1938:1943	intestinal epithelial HIF-1α	1916:1943	intestinal epithelial HIF-1α	1916:1943	KEY MESSAGES: • The absence of intestinal epithelial HIF-1α leads to downregulation of autophagy in mice.
32696223	9	7	theme	colonic	1376:1382	arg1	injury					1384:1389	the colonic injury	1372:1389	the colonic injury	1372:1389	Furthermore, in the DSS-induced colitis model, butyrate administration relieved the colonic injury and suppressed inflammation in Cre-/HIF-1α- (HIF-1αloxP/loxP) mice.
32696223	12	8	theme	DSS-induced	1727:1737	arg1	colitis					1739:1745	DSS-induced colitis	1727:1745	DSS-induced colitis	1727:1745	Butyrate can alleviate DSS-induced colitis by regulating autophagy via HIF-1α.
32696223	7	9	theme	HIF-1α-deficient	1055:1070	arg1	mice					1085:1088	IEC-specific HIF-1α-deficient (HIF-1α∆IEC) mice	1042:1088	IEC-specific HIF-1α-deficient (HIF-1α∆IEC) mice	1042:1088	In addition, autophagy levels in the intestinal epithelial cells (IECs) were significantly reduced in IEC-specific HIF-1α-deficient (HIF-1α∆IEC) mice.
32696223	15	10	theme	DSS-induced	2049:2059	arg1	colitis					2061:2067	DSS-induced colitis	2049:2067	DSS-induced colitis	2049:2067	• The absence of intestinal epithelial HIF-1α exacerbates DSS-induced colitis.
32696223	10	11	theme	HIF-1α∆IEC	1559:1568	arg1	mice					1570:1573	the HIF-1α∆IEC mice	1555:1573	the HIF-1α∆IEC mice	1555:1573	However, the butyrate-mediated protection against colonic injury was considerably diminished in the HIF-1α∆IEC mice.
32696223	15	12	theme	epithelial	2019:2028	arg1	HIF-1α					2030:2035	intestinal epithelial HIF-1α	2008:2035	intestinal epithelial HIF-1α	2008:2035	• The absence of intestinal epithelial HIF-1α exacerbates DSS-induced colitis.
32696223	14	13	theme	epithelial	1927:1936	arg1	HIF-1α					1938:1943	intestinal epithelial HIF-1α	1916:1943	intestinal epithelial HIF-1α	1916:1943	KEY MESSAGES: • The absence of intestinal epithelial HIF-1α leads to downregulation of autophagy in mice.
32696223	5	14	theme	colitis	696:702	arg1	models					704:709	colitis models	696:709	colitis models	696:709	In this study, we investigated the relationship between HIF-1α and autophagy in healthy and inflammatory states using transgenic mice, colitis models, and cell culture models.
32696223	8	15	from	autophagy	1172:1180	arg1	cells					1190:1194	HT29 cells	1185:1194	HT29 cells	1185:1194	Moreover, in the cell culture models, butyrate treatment significantly increased autophagy in HT29 cells under normal conditions, whereas butyrate had little effect on autophagy after HIF-1α ablation.
32696223	8	16	theme	culture	1113:1119	arg1	models					1121:1126	the cell culture models	1104:1126	the cell culture models	1104:1126	Moreover, in the cell culture models, butyrate treatment significantly increased autophagy in HT29 cells under normal conditions, whereas butyrate had little effect on autophagy after HIF-1α ablation.
32696223	13	17	contain	have	1802:1805	arg2	implications					1817:1828	important implications	1807:1828	important implications for the development of therapeutic strategies for IBD	1807:1882	These insights may have important implications for the development of therapeutic strategies for IBD.
32696223	13	17	contain	have	1802:1805	arg1	insights					1789:1796	These insights	1783:1796	These insights	1783:1796	These insights may have important implications for the development of therapeutic strategies for IBD.
32696223	1	18	theme	barrier	161:167	arg1	integrity					169:177	barrier integrity	161:177	barrier integrity	161:177	Hypoxia-inducible factor-1α (HIF-1α) is a critical regulator of barrier integrity during colonic mucosal injury.
32696223	8	19	theme	cell	1108:1111	arg1	models					1121:1126	the cell culture models	1104:1126	the cell culture models	1104:1126	Moreover, in the cell culture models, butyrate treatment significantly increased autophagy in HT29 cells under normal conditions, whereas butyrate had little effect on autophagy after HIF-1α ablation.
32696223	3	20	from	profiles	371:378	arg1	gut					387:389	the gut	383:389	the gut	383:389	Additionally, changes in bacterial profiles in the gut are intimately associated with IBD.
32696223	7	21	theme	IEC-specific	1042:1053	arg1	mice					1085:1088	IEC-specific HIF-1α-deficient (HIF-1α∆IEC) mice	1042:1088	IEC-specific HIF-1α-deficient (HIF-1α∆IEC) mice	1042:1088	In addition, autophagy levels in the intestinal epithelial cells (IECs) were significantly reduced in IEC-specific HIF-1α-deficient (HIF-1α∆IEC) mice.
32696223	15	22	theme	The	1993:1995	arg1	absence					1997:2003	• The absence	1991:2003	• The absence of intestinal epithelial HIF-1α	1991:2035	• The absence of intestinal epithelial HIF-1α exacerbates DSS-induced colitis.
32696223	9	23	theme	butyrate	1339:1346	arg1	administration					1348:1361	butyrate administration	1339:1361	butyrate administration	1339:1361	Furthermore, in the DSS-induced colitis model, butyrate administration relieved the colonic injury and suppressed inflammation in Cre-/HIF-1α- (HIF-1αloxP/loxP) mice.
32696223	1	24	theme	integrity	169:177	arg1	regulator					148:156	a critical regulator	137:156	a critical regulator of barrier integrity during colonic mucosal injury	137:207	Hypoxia-inducible factor-1α (HIF-1α) is a critical regulator of barrier integrity during colonic mucosal injury.
32696223	1	24	theme	integrity	169:177	arg1	factor-1α					115:123	Hypoxia-inducible factor-1α	97:123	Hypoxia-inducible factor-1α (HIF-1α)	97:132	Hypoxia-inducible factor-1α (HIF-1α) is a critical regulator of barrier integrity during colonic mucosal injury.
32696223	6	25	theme	intestinal	830:839	arg1	microbes					841:848	the intestinal microbes	826:848	the intestinal microbes	826:848	We confirmed that the absence of intestinal epithelial HIF-1α changed the composition of the intestinal microbes and increased the susceptibility of mice to dextran sodium sulfate (DSS)-induced colitis.
32696223	6	26	theme	-induced	922:929	arg1	colitis					931:937	dextran sodium sulfate (DSS)-induced colitis	894:937	dextran sodium sulfate (DSS)-induced colitis	894:937	We confirmed that the absence of intestinal epithelial HIF-1α changed the composition of the intestinal microbes and increased the susceptibility of mice to dextran sodium sulfate (DSS)-induced colitis.
32696223	2	27	theme	bowel	315:319	arg1	IBD					330:332	IBD	330:332	IBD	330:332	Previous works have shown that the absence of autophagy is implicated in the development of inflammatory bowel disease (IBD).
32696223	2	27	theme	bowel	315:319	arg1	disease					321:327	inflammatory bowel disease	302:327	inflammatory bowel disease (IBD)	302:333	Previous works have shown that the absence of autophagy is implicated in the development of inflammatory bowel disease (IBD).
32696223	16	28	theme	•	2070:2070	arg1	SCFAs					2097:2101	SCFAs	2097:2101	SCFAs	2097:2101	• Short-chain fatty acids (SCFAs) can alleviate DSS-induced colitis by regulating autophagy via HIF-1α.
32696223	16	28	theme	•	2070:2070	arg1	acids					2090:2094	• Short-chain fatty acids	2070:2094	• Short-chain fatty acids (SCFAs)	2070:2102	• Short-chain fatty acids (SCFAs) can alleviate DSS-induced colitis by regulating autophagy via HIF-1α.
32696223	8	29	theme	normal	1202:1207	arg1	conditions					1209:1218	normal conditions	1202:1218	normal conditions	1202:1218	Moreover, in the cell culture models, butyrate treatment significantly increased autophagy in HT29 cells under normal conditions, whereas butyrate had little effect on autophagy after HIF-1α ablation.
32696223	15	30	theme	•	1991:1991	arg1	absence					1997:2003	• The absence	1991:2003	• The absence of intestinal epithelial HIF-1α	1991:2035	• The absence of intestinal epithelial HIF-1α exacerbates DSS-induced colitis.
32696223	4	31	theme	IBD	529:531	arg1	pathogenesis					513:524	the pathogenesis	509:524	the pathogenesis of IBD	509:531	Although HIF-1α, autophagy, microbiota, and their metabolites are all involved in the pathogenesis of IBD, their roles are not known.
32696223	2	32	theme	inflammatory	302:313	arg1	IBD					330:332	IBD	330:332	IBD	330:332	Previous works have shown that the absence of autophagy is implicated in the development of inflammatory bowel disease (IBD).
32696223	2	32	theme	inflammatory	302:313	arg1	disease					321:327	inflammatory bowel disease	302:327	inflammatory bowel disease (IBD)	302:333	Previous works have shown that the absence of autophagy is implicated in the development of inflammatory bowel disease (IBD).
32696223	1	33	theme	colonic	186:192	arg1	injury					202:207	colonic mucosal injury	186:207	colonic mucosal injury	186:207	Hypoxia-inducible factor-1α (HIF-1α) is a critical regulator of barrier integrity during colonic mucosal injury.
32696223	5	34	theme	inflammatory	653:664	arg1	states					666:671	healthy and inflammatory states	641:671	healthy and inflammatory states	641:671	In this study, we investigated the relationship between HIF-1α and autophagy in healthy and inflammatory states using transgenic mice, colitis models, and cell culture models.
32696223	1	35	theme	Hypoxia-inducible	97:113	arg1	regulator					148:156	a critical regulator	137:156	a critical regulator of barrier integrity during colonic mucosal injury	137:207	Hypoxia-inducible factor-1α (HIF-1α) is a critical regulator of barrier integrity during colonic mucosal injury.
32696223	1	35	theme	Hypoxia-inducible	97:113	arg1	HIF-1α					126:131	HIF-1α	126:131	HIF-1α	126:131	Hypoxia-inducible factor-1α (HIF-1α) is a critical regulator of barrier integrity during colonic mucosal injury.
32696223	1	35	theme	Hypoxia-inducible	97:113	arg1	factor-1α					115:123	Hypoxia-inducible factor-1α	97:123	Hypoxia-inducible factor-1α (HIF-1α)	97:132	Hypoxia-inducible factor-1α (HIF-1α) is a critical regulator of barrier integrity during colonic mucosal injury.
32696223	1	36	theme	mucosal	194:200	arg1	injury					202:207	colonic mucosal injury	186:207	colonic mucosal injury	186:207	Hypoxia-inducible factor-1α (HIF-1α) is a critical regulator of barrier integrity during colonic mucosal injury.
32696223	3	37	theme	bacterial	361:369	arg1	profiles					371:378	bacterial profiles	361:378	bacterial profiles in the gut	361:389	Additionally, changes in bacterial profiles in the gut are intimately associated with IBD.
32696223	16	38	theme	DSS-induced	2118:2128	arg1	colitis					2130:2136	DSS-induced colitis	2118:2136	DSS-induced colitis	2118:2136	• Short-chain fatty acids (SCFAs) can alleviate DSS-induced colitis by regulating autophagy via HIF-1α.
32696223	15	39	theme	HIF-1α	2030:2035	arg1	absence					1997:2003	• The absence	1991:2003	• The absence of intestinal epithelial HIF-1α	1991:2035	• The absence of intestinal epithelial HIF-1α exacerbates DSS-induced colitis.
32696223	11	40	theme	intestinal	1680:1689	arg1	homeostasis					1691:1701	intestinal homeostasis	1680:1701	intestinal homeostasis	1680:1701	These results show that HIF-1α, autophagy, and intestinal microbes are essential for the maintenance of intestinal homeostasis.
32696223	13	41	theme	strategies	1865:1874	arg1	development					1838:1848	the development	1834:1848	the development of therapeutic strategies for IBD	1834:1882	These insights may have important implications for the development of therapeutic strategies for IBD.
32696223	13	42	theme	therapeutic	1853:1863	arg1	strategies					1865:1874	therapeutic strategies	1853:1874	therapeutic strategies for IBD	1853:1882	These insights may have important implications for the development of therapeutic strategies for IBD.
32696223	9	43	theme	HIF-1αloxP/loxP	1436:1450	arg1	mice					1453:1456	Cre-/HIF-1α- (HIF-1αloxP/loxP) mice	1422:1456	Cre-/HIF-1α- (HIF-1αloxP/loxP) mice	1422:1456	Furthermore, in the DSS-induced colitis model, butyrate administration relieved the colonic injury and suppressed inflammation in Cre-/HIF-1α- (HIF-1αloxP/loxP) mice.
32696223	0	44	theme	epithelial	37:46	arg1	cells					48:52	intestinal epithelial cells	26:52	intestinal epithelial cells	26:52	SCFAs induce autophagy in intestinal epithelial cells and relieve colitis by stabilizing HIF-1α.
32696223	14	45	theme	KEY	1885:1887	arg1	MESSAGES					1889:1896	KEY MESSAGES	1885:1896	KEY MESSAGES: • The absence of intestinal epithelial HIF-1α leads to downregulation of autophagy in mice.	1885:1989	KEY MESSAGES: • The absence of intestinal epithelial HIF-1α leads to downregulation of autophagy in mice.
32696223	11	46	theme	homeostasis	1691:1701	arg1	maintenance					1665:1675	the maintenance	1661:1675	the maintenance of intestinal homeostasis	1661:1701	These results show that HIF-1α, autophagy, and intestinal microbes are essential for the maintenance of intestinal homeostasis.
32696223	9	47	theme	Cre-/HIF-1α-	1422:1433	arg1	mice					1453:1456	Cre-/HIF-1α- (HIF-1αloxP/loxP) mice	1422:1456	Cre-/HIF-1α- (HIF-1αloxP/loxP) mice	1422:1456	Furthermore, in the DSS-induced colitis model, butyrate administration relieved the colonic injury and suppressed inflammation in Cre-/HIF-1α- (HIF-1αloxP/loxP) mice.
32696223	7	48	from	levels	963:968	arg1	IECs					1006:1009	IECs	1006:1009	IECs	1006:1009	In addition, autophagy levels in the intestinal epithelial cells (IECs) were significantly reduced in IEC-specific HIF-1α-deficient (HIF-1α∆IEC) mice.
32696223	7	48	from	levels	963:968	arg1	cells					999:1003	the intestinal epithelial cells	973:1003	the intestinal epithelial cells (IECs)	973:1010	In addition, autophagy levels in the intestinal epithelial cells (IECs) were significantly reduced in IEC-specific HIF-1α-deficient (HIF-1α∆IEC) mice.
32696223	0	49	theme	intestinal	26:35	arg1	cells					48:52	intestinal epithelial cells	26:52	intestinal epithelial cells	26:52	SCFAs induce autophagy in intestinal epithelial cells and relieve colitis by stabilizing HIF-1α.
32696223	5	50	from	HIF-1α	617:622	arg1	states					666:671	healthy and inflammatory states	641:671	healthy and inflammatory states	641:671	In this study, we investigated the relationship between HIF-1α and autophagy in healthy and inflammatory states using transgenic mice, colitis models, and cell culture models.
32696223	5	51	theme	transgenic	679:688	arg1	mice					690:693	transgenic mice	679:693	transgenic mice	679:693	In this study, we investigated the relationship between HIF-1α and autophagy in healthy and inflammatory states using transgenic mice, colitis models, and cell culture models.
32696223	9	52	theme	DSS-induced	1312:1322	arg1	model					1332:1336	the DSS-induced colitis model	1308:1336	the DSS-induced colitis model	1308:1336	Furthermore, in the DSS-induced colitis model, butyrate administration relieved the colonic injury and suppressed inflammation in Cre-/HIF-1α- (HIF-1αloxP/loxP) mice.
32696223	7	53	theme	epithelial	988:997	arg1	IECs					1006:1009	IECs	1006:1009	IECs	1006:1009	In addition, autophagy levels in the intestinal epithelial cells (IECs) were significantly reduced in IEC-specific HIF-1α-deficient (HIF-1α∆IEC) mice.
32696223	7	53	theme	epithelial	988:997	arg1	cells					999:1003	the intestinal epithelial cells	973:1003	the intestinal epithelial cells (IECs)	973:1010	In addition, autophagy levels in the intestinal epithelial cells (IECs) were significantly reduced in IEC-specific HIF-1α-deficient (HIF-1α∆IEC) mice.
32696223	3	54	from	changes	350:356	arg1	profiles					371:378	bacterial profiles	361:378	bacterial profiles in the gut	361:389	Additionally, changes in bacterial profiles in the gut are intimately associated with IBD.
32696223	14	55	theme	autophagy	1972:1980	arg1	downregulation					1954:1967	downregulation	1954:1967	downregulation of autophagy in mice	1954:1988	KEY MESSAGES: • The absence of intestinal epithelial HIF-1α leads to downregulation of autophagy in mice.
32696223	6	56	theme	microbes	841:848	arg1	composition					811:821	the composition	807:821	the composition of the intestinal microbes	807:848	We confirmed that the absence of intestinal epithelial HIF-1α changed the composition of the intestinal microbes and increased the susceptibility of mice to dextran sodium sulfate (DSS)-induced colitis.
32696223	8	57	theme	little	1242:1247	arg1	effect					1249:1254	little effect	1242:1254	little effect	1242:1254	Moreover, in the cell culture models, butyrate treatment significantly increased autophagy in HT29 cells under normal conditions, whereas butyrate had little effect on autophagy after HIF-1α ablation.
32696223	15	58	theme	intestinal	2008:2017	arg1	HIF-1α					2030:2035	intestinal epithelial HIF-1α	2008:2035	intestinal epithelial HIF-1α	2008:2035	• The absence of intestinal epithelial HIF-1α exacerbates DSS-induced colitis.
32696223	9	59	theme	colitis	1324:1330	arg1	model					1332:1336	the DSS-induced colitis model	1308:1336	the DSS-induced colitis model	1308:1336	Furthermore, in the DSS-induced colitis model, butyrate administration relieved the colonic injury and suppressed inflammation in Cre-/HIF-1α- (HIF-1αloxP/loxP) mice.
32696223	11	60	theme	intestinal	1623:1632	arg1	microbes					1634:1641	intestinal microbes	1623:1641	intestinal microbes	1623:1641	These results show that HIF-1α, autophagy, and intestinal microbes are essential for the maintenance of intestinal homeostasis.
32696223	2	61	theme	Previous	210:217	arg1	works					219:223	Previous works	210:223	Previous works	210:223	Previous works have shown that the absence of autophagy is implicated in the development of inflammatory bowel disease (IBD).
32696223	14	62	theme	HIF-1α	1938:1943	arg1	absence					1905:1911	The absence	1901:1911	The absence of intestinal epithelial HIF-1α	1901:1943	KEY MESSAGES: • The absence of intestinal epithelial HIF-1α leads to downregulation of autophagy in mice.
32696223	5	63	theme	healthy	641:647	arg1	states					666:671	healthy and inflammatory states	641:671	healthy and inflammatory states	641:671	In this study, we investigated the relationship between HIF-1α and autophagy in healthy and inflammatory states using transgenic mice, colitis models, and cell culture models.
32696223	6	64	theme	HIF-1α	792:797	arg1	absence					759:765	the absence	755:765	the absence of intestinal epithelial HIF-1α	755:797	We confirmed that the absence of intestinal epithelial HIF-1α changed the composition of the intestinal microbes and increased the susceptibility of mice to dextran sodium sulfate (DSS)-induced colitis.
32696223	2	65	theme	autophagy	256:264	arg1	absence					245:251	the absence	241:251	the absence of autophagy	241:264	Previous works have shown that the absence of autophagy is implicated in the development of inflammatory bowel disease (IBD).
32696223	7	66	theme	autophagy	953:961	arg1	levels					963:968	autophagy levels	953:968	autophagy levels in the intestinal epithelial cells (IECs)	953:1010	In addition, autophagy levels in the intestinal epithelial cells (IECs) were significantly reduced in IEC-specific HIF-1α-deficient (HIF-1α∆IEC) mice.
32696223	16	67	theme	Short-chain	2072:2082	arg1	SCFAs					2097:2101	SCFAs	2097:2101	SCFAs	2097:2101	• Short-chain fatty acids (SCFAs) can alleviate DSS-induced colitis by regulating autophagy via HIF-1α.
32696223	16	67	theme	Short-chain	2072:2082	arg1	acids					2090:2094	• Short-chain fatty acids	2070:2094	• Short-chain fatty acids (SCFAs)	2070:2102	• Short-chain fatty acids (SCFAs) can alleviate DSS-induced colitis by regulating autophagy via HIF-1α.
32696223	7	68	theme	intestinal	977:986	arg1	IECs					1006:1009	IECs	1006:1009	IECs	1006:1009	In addition, autophagy levels in the intestinal epithelial cells (IECs) were significantly reduced in IEC-specific HIF-1α-deficient (HIF-1α∆IEC) mice.
32696223	7	68	theme	intestinal	977:986	arg1	cells					999:1003	the intestinal epithelial cells	973:1003	the intestinal epithelial cells (IECs)	973:1010	In addition, autophagy levels in the intestinal epithelial cells (IECs) were significantly reduced in IEC-specific HIF-1α-deficient (HIF-1α∆IEC) mice.
32696223	6	69	theme	epithelial	781:790	arg1	HIF-1α					792:797	intestinal epithelial HIF-1α	770:797	intestinal epithelial HIF-1α	770:797	We confirmed that the absence of intestinal epithelial HIF-1α changed the composition of the intestinal microbes and increased the susceptibility of mice to dextran sodium sulfate (DSS)-induced colitis.
32696223	2	70	theme	disease	321:327	arg1	development					287:297	the development	283:297	the development of inflammatory bowel disease (IBD)	283:333	Previous works have shown that the absence of autophagy is implicated in the development of inflammatory bowel disease (IBD).
32696223	8	71	contain	had	1238:1240	arg2	effect					1249:1254	little effect	1242:1254	little effect	1242:1254	Moreover, in the cell culture models, butyrate treatment significantly increased autophagy in HT29 cells under normal conditions, whereas butyrate had little effect on autophagy after HIF-1α ablation.
32696223	8	71	contain	had	1238:1240	arg1	butyrate					1229:1236	butyrate	1229:1236	butyrate	1229:1236	Moreover, in the cell culture models, butyrate treatment significantly increased autophagy in HT29 cells under normal conditions, whereas butyrate had little effect on autophagy after HIF-1α ablation.
32696223	13	72	theme	important	1807:1815	arg1	implications					1817:1828	important implications	1807:1828	important implications for the development of therapeutic strategies for IBD	1807:1882	These insights may have important implications for the development of therapeutic strategies for IBD.
32696223	6	73	theme	intestinal	770:779	arg1	HIF-1α					792:797	intestinal epithelial HIF-1α	770:797	intestinal epithelial HIF-1α	770:797	We confirmed that the absence of intestinal epithelial HIF-1α changed the composition of the intestinal microbes and increased the susceptibility of mice to dextran sodium sulfate (DSS)-induced colitis.
32696223	14	74	dep	MESSAGES	1889:1896	arg1	leads					1945:1949	leads	1945:1949	leads to downregulation of autophagy in mice	1945:1988	KEY MESSAGES: • The absence of intestinal epithelial HIF-1α leads to downregulation of autophagy in mice.
32696223	8	75	theme	HT29	1185:1188	arg1	cells					1190:1194	HT29 cells	1185:1194	HT29 cells	1185:1194	Moreover, in the cell culture models, butyrate treatment significantly increased autophagy in HT29 cells under normal conditions, whereas butyrate had little effect on autophagy after HIF-1α ablation.
32696223	5	76	from	autophagy	628:636	arg1	states					666:671	healthy and inflammatory states	641:671	healthy and inflammatory states	641:671	In this study, we investigated the relationship between HIF-1α and autophagy in healthy and inflammatory states using transgenic mice, colitis models, and cell culture models.
32696223	6	77	theme	mice	886:889	arg1	susceptibility					868:881	the susceptibility	864:881	the susceptibility of mice to dextran sodium sulfate (DSS)-induced colitis	864:937	We confirmed that the absence of intestinal epithelial HIF-1α changed the composition of the intestinal microbes and increased the susceptibility of mice to dextran sodium sulfate (DSS)-induced colitis.
32696223	8	78	theme	butyrate	1129:1136	arg1	treatment					1138:1146	butyrate treatment	1129:1146	butyrate treatment	1129:1146	Moreover, in the cell culture models, butyrate treatment significantly increased autophagy in HT29 cells under normal conditions, whereas butyrate had little effect on autophagy after HIF-1α ablation.
32696223	14	79	from	downregulation	1954:1967	arg1	mice					1985:1988	mice	1985:1988	mice	1985:1988	KEY MESSAGES: • The absence of intestinal epithelial HIF-1α leads to downregulation of autophagy in mice.
32696223	1	80	theme	critical	139:146	arg1	regulator					148:156	a critical regulator	137:156	a critical regulator of barrier integrity during colonic mucosal injury	137:207	Hypoxia-inducible factor-1α (HIF-1α) is a critical regulator of barrier integrity during colonic mucosal injury.
32696223	1	80	theme	critical	139:146	arg1	factor-1α					115:123	Hypoxia-inducible factor-1α	97:123	Hypoxia-inducible factor-1α (HIF-1α)	97:132	Hypoxia-inducible factor-1α (HIF-1α) is a critical regulator of barrier integrity during colonic mucosal injury.
32696223	16	81	theme	fatty	2084:2088	arg1	SCFAs					2097:2101	SCFAs	2097:2101	SCFAs	2097:2101	• Short-chain fatty acids (SCFAs) can alleviate DSS-induced colitis by regulating autophagy via HIF-1α.
32696223	16	81	theme	fatty	2084:2088	arg1	acids					2090:2094	• Short-chain fatty acids	2070:2094	• Short-chain fatty acids (SCFAs)	2070:2102	• Short-chain fatty acids (SCFAs) can alleviate DSS-induced colitis by regulating autophagy via HIF-1α.
32692642	2	0	theme	Pantholops	101:110	arg1	hodgsonii					112:120	Pantholops hodgsonii	101:120	Pantholops hodgsonii	101:120	nov., isolated from Tibetan antelope (Pantholops hodgsonii) and plateau pika (Ochotona curzoniae), respectively.
32692642	2	0	theme	Pantholops	101:110	arg1	antelope					91:98	Tibetan antelope	83:98	Tibetan antelope (Pantholops hodgsonii)	83:121	nov., isolated from Tibetan antelope (Pantholops hodgsonii) and plateau pika (Ochotona curzoniae), respectively.
32692642	4	1	dep	catalase-positive	391:407	arg1	rod-shaped					410:419	rod-shaped	410:419	rod-shaped	410:419	The cells were aerobic, non-motile, Gram-stain- and catalase-positive, rod-shaped bacteria.
32692642	5	2	theme	16S	435:437	arg1	sequences					449:457	The 16S rRNA gene sequences	431:457	The 16S rRNA gene sequences of the four strains	431:477	The 16S rRNA gene sequences of the four strains showed highest similarities to Aeromicrobium fastidiosum DSM 10552T (98.1, 98.6, 98.7 and 98.7 %, respectively), and the phylogenetic analyses based on 16S rRNA gene and genomic sequences indicated that strains 592T and MF47T represent two novel species.
32692642	9	3	theme	Aeromicrobium	1292:1304	arg1	14732T					1323:1328	Aeromicrobium ginsengisoli JCM 14732T	1292:1328	Aeromicrobium ginsengisoli JCM 14732T	1292:1328	The four strains' genomes displayed less than 24.6 % DNA-DNA relatedness with all available genomes of the genus Aeromicrobium in the NCBI database, including Aeromicrobium fastidiosum NBRC 14897T and Aeromicrobium ginsengisoli JCM 14732T.
32692642	8	4	theme	%	1043:1043	arg1	threshold					1027:1035	the threshold	1023:1035	the threshold of 70 %	1023:1043	The digital DNA-DNA hybridization value between strains 592T and MF47T was 32.6 %, lower than the threshold of 70 %, indicating they belong to different species.
32692642	12	5	theme	ll-diaminopimelic	1618:1634	arg1	acid					1636:1639	ll-diaminopimelic acid	1618:1639	ll-diaminopimelic acid	1618:1639	The cell-wall peptidoglycan contained ll-diaminopimelic acid.
32692642	2	6	attach	isolated	69:76	arg1	hodgsonii					112:120	Pantholops hodgsonii	101:120	Pantholops hodgsonii	101:120	nov., isolated from Tibetan antelope (Pantholops hodgsonii) and plateau pika (Ochotona curzoniae), respectively.
32692642	2	6	attach	isolated	69:76	arg1	Ochotona					141:148	Ochotona	141:148	Ochotona	141:148	nov., isolated from Tibetan antelope (Pantholops hodgsonii) and plateau pika (Ochotona curzoniae), respectively.
32692642	2	6	attach	isolated	69:76	arg1	antelope					91:98	Tibetan antelope	83:98	Tibetan antelope (Pantholops hodgsonii)	83:121	nov., isolated from Tibetan antelope (Pantholops hodgsonii) and plateau pika (Ochotona curzoniae), respectively.
32692642	2	6	attach	isolated	69:76	arg1	pika					135:138	plateau pika	127:138	plateau pika (Ochotona curzoniae)	127:159	nov., isolated from Tibetan antelope (Pantholops hodgsonii) and plateau pika (Ochotona curzoniae), respectively.
32692642	2	6	attach	isolated	69:76	arg2	nov.					63:66	nov.	63:66	nov.	63:66	nov., isolated from Tibetan antelope (Pantholops hodgsonii) and plateau pika (Ochotona curzoniae), respectively.
32692642	10	7	theme	 0	1399:1400	arg1	10-methyl					1402:1410	 0 10-methyl	1399:1410	C18 : 1 ω9c and C18 : 0 10-methyl	1378:1410	The major fatty acids of the four strains were C18 : 1 ω9c and C18 : 0 10-methyl, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol.
32692642	2	8	theme	plateau	127:133	arg1	Ochotona					141:148	Ochotona	141:148	Ochotona	141:148	nov., isolated from Tibetan antelope (Pantholops hodgsonii) and plateau pika (Ochotona curzoniae), respectively.
32692642	2	8	theme	plateau	127:133	arg1	pika					135:138	plateau pika	127:138	plateau pika (Ochotona curzoniae)	127:159	nov., isolated from Tibetan antelope (Pantholops hodgsonii) and plateau pika (Ochotona curzoniae), respectively.
32692642	14	9	dep	sp	1851:1852	arg1	Aeromicrobium					1829:1841	Aeromicrobium	1829:1841	Aeromicrobium	1829:1841	nov. and Aeromicrobium yanjiei sp.
32692642	14	9	dep	sp	1851:1852	arg1	nov.					1820:1823	nov.	1820:1823	nov.	1820:1823	nov. and Aeromicrobium yanjiei sp.
32692642	5	10	theme	gene	444:447	arg1	sequences					449:457	The 16S rRNA gene sequences	431:457	The 16S rRNA gene sequences of the four strains	431:477	The 16S rRNA gene sequences of the four strains showed highest similarities to Aeromicrobium fastidiosum DSM 10552T (98.1, 98.6, 98.7 and 98.7 %, respectively), and the phylogenetic analyses based on 16S rRNA gene and genomic sequences indicated that strains 592T and MF47T represent two novel species.
32692642	9	11	theme	JCM	1319:1321	arg1	14732T					1323:1328	Aeromicrobium ginsengisoli JCM 14732T	1292:1328	Aeromicrobium ginsengisoli JCM 14732T	1292:1328	The four strains' genomes displayed less than 24.6 % DNA-DNA relatedness with all available genomes of the genus Aeromicrobium in the NCBI database, including Aeromicrobium fastidiosum NBRC 14897T and Aeromicrobium ginsengisoli JCM 14732T.
32692642	10	12	theme	polar	1426:1430	arg1	diphosphatidylglycerol					1444:1465	diphosphatidylglycerol	1444:1465	diphosphatidylglycerol	1444:1465	The major fatty acids of the four strains were C18 : 1 ω9c and C18 : 0 10-methyl, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol.
32692642	10	12	theme	polar	1426:1430	arg1	lipids					1432:1437	the main polar lipids	1417:1437	the main polar lipids	1417:1437	The major fatty acids of the four strains were C18 : 1 ω9c and C18 : 0 10-methyl, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol.
32692642	13	13	theme	Aeromicrobium	1792:1804	arg1	species					1783:1789	two novel species	1773:1789	two novel species	1773:1789	Based on these genotypic, phenotypic and biochemical analyses, it is proposed that the four unidentified bacteria be classified as two novel species, Aeromicrobium chenweiae sp.
32692642	13	13	theme	Aeromicrobium	1792:1804	arg1	sp					1816:1817	Aeromicrobium chenweiae sp	1792:1817	Aeromicrobium chenweiae sp	1792:1817	Based on these genotypic, phenotypic and biochemical analyses, it is proposed that the four unidentified bacteria be classified as two novel species, Aeromicrobium chenweiae sp.
32692642	13	14	theme	biochemical	1683:1693	arg1	analyses					1695:1702	these genotypic, phenotypic and biochemical analyses	1651:1702	these genotypic, phenotypic and biochemical analyses	1651:1702	Based on these genotypic, phenotypic and biochemical analyses, it is proposed that the four unidentified bacteria be classified as two novel species, Aeromicrobium chenweiae sp.
32692642	8	15	theme	different	1072:1080	arg1	species					1082:1088	different species	1072:1088	different species	1072:1088	The digital DNA-DNA hybridization value between strains 592T and MF47T was 32.6 %, lower than the threshold of 70 %, indicating they belong to different species.
32692642	5	16	theme	16S	631:633	arg1	gene					640:643	16S rRNA gene	631:643	16S rRNA gene	631:643	The 16S rRNA gene sequences of the four strains showed highest similarities to Aeromicrobium fastidiosum DSM 10552T (98.1, 98.6, 98.7 and 98.7 %, respectively), and the phylogenetic analyses based on 16S rRNA gene and genomic sequences indicated that strains 592T and MF47T represent two novel species.
32692642	7	17	theme	69.8 mol	904:911	arg1	contents					855:862	The DNA G+C contents	843:862	The DNA G+C contents of strains 592T and MF47T	843:888	The DNA G+C contents of strains 592T and MF47T were 70.3 and 69.8 mol%, respectively.
32692642	7	17	theme	69.8 mol	904:911	arg1	%					912:912	70.3 and 69.8 mol%	895:912	70.3 and 69.8 mol%	895:912	The DNA G+C contents of strains 592T and MF47T were 70.3 and 69.8 mol%, respectively.
32692642	4	18	theme	catalase-positive	391:407	arg1	bacteria					421:428	catalase-positive, rod-shaped bacteria	391:428	catalase-positive, rod-shaped bacteria	391:428	The cells were aerobic, non-motile, Gram-stain- and catalase-positive, rod-shaped bacteria.
32692642	10	19	theme	strains	1365:1371	arg1	C18 					1378:1381	C18 	1378:1381	C18 	1378:1381	The major fatty acids of the four strains were C18 : 1 ω9c and C18 : 0 10-methyl, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol.
32692642	10	19	theme	strains	1365:1371	arg1	acids					1347:1351	The major fatty acids	1331:1351	The major fatty acids of the four strains	1331:1371	The major fatty acids of the four strains were C18 : 1 ω9c and C18 : 0 10-methyl, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol.
32692642	1	20	dep	sp	59:60	arg1	Aeromicrobium					37:49	Aeromicrobium	37:49	Aeromicrobium	37:49	nov. and Aeromicrobium yanjiei sp.
32692642	1	20	dep	sp	59:60	arg1	nov.					28:31	nov.	28:31	nov.	28:31	nov. and Aeromicrobium yanjiei sp.
32692642	16	21	theme	type	1864:1867	arg1	592T					1881:1884	592T	1881:1884	592T (=CGMCC1.16526T=DSM 106289T)	1881:1913	The type strains are 592T (=CGMCC1.16526T=DSM 106289T) and MF47T (=CGMCC 1.17444T=JCM 33790T), respectively.
32692642	16	21	theme	type	1864:1867	arg1	strains					1869:1875	The type strains	1860:1875	The type strains	1860:1875	The type strains are 592T (=CGMCC1.16526T=DSM 106289T) and MF47T (=CGMCC 1.17444T=JCM 33790T), respectively.
32692642	5	22	theme	phylogenetic	600:611	arg1	analyses					613:620	the phylogenetic analyses	596:620	the phylogenetic analyses based on 16S rRNA gene and genomic sequences	596:665	The 16S rRNA gene sequences of the four strains showed highest similarities to Aeromicrobium fastidiosum DSM 10552T (98.1, 98.6, 98.7 and 98.7 %, respectively), and the phylogenetic analyses based on 16S rRNA gene and genomic sequences indicated that strains 592T and MF47T represent two novel species.
32692642	13	23	theme	novel	1777:1781	arg1	bacteria					1747:1754	the four unidentified bacteria	1725:1754	the four unidentified bacteria	1725:1754	Based on these genotypic, phenotypic and biochemical analyses, it is proposed that the four unidentified bacteria be classified as two novel species, Aeromicrobium chenweiae sp.
32692642	13	23	theme	novel	1777:1781	arg1	species					1783:1789	two novel species	1773:1789	two novel species	1773:1789	Based on these genotypic, phenotypic and biochemical analyses, it is proposed that the four unidentified bacteria be classified as two novel species, Aeromicrobium chenweiae sp.
32692642	13	23	theme	novel	1777:1781	arg1	sp					1816:1817	Aeromicrobium chenweiae sp	1792:1817	Aeromicrobium chenweiae sp	1792:1817	Based on these genotypic, phenotypic and biochemical analyses, it is proposed that the four unidentified bacteria be classified as two novel species, Aeromicrobium chenweiae sp.
32692642	7	24	theme	strains	867:873	arg1	contents					855:862	The DNA G+C contents	843:862	The DNA G+C contents of strains 592T and MF47T	843:888	The DNA G+C contents of strains 592T and MF47T were 70.3 and 69.8 mol%, respectively.
32692642	7	24	theme	strains	867:873	arg1	%					912:912	70.3 and 69.8 mol%	895:912	70.3 and 69.8 mol%	895:912	The DNA G+C contents of strains 592T and MF47T were 70.3 and 69.8 mol%, respectively.
32692642	13	25	theme	unidentified	1734:1745	arg1	bacteria					1747:1754	the four unidentified bacteria	1725:1754	the four unidentified bacteria	1725:1754	Based on these genotypic, phenotypic and biochemical analyses, it is proposed that the four unidentified bacteria be classified as two novel species, Aeromicrobium chenweiae sp.
32692642	13	25	theme	unidentified	1734:1745	arg1	species					1783:1789	two novel species	1773:1789	two novel species	1773:1789	Based on these genotypic, phenotypic and biochemical analyses, it is proposed that the four unidentified bacteria be classified as two novel species, Aeromicrobium chenweiae sp.
32692642	9	26	theme	DNA-DNA	1144:1150	arg1	relatedness					1152:1162	less than 24.6 % DNA-DNA relatedness	1127:1162	less than 24.6 % DNA-DNA relatedness	1127:1162	The four strains' genomes displayed less than 24.6 % DNA-DNA relatedness with all available genomes of the genus Aeromicrobium in the NCBI database, including Aeromicrobium fastidiosum NBRC 14897T and Aeromicrobium ginsengisoli JCM 14732T.
32692642	9	27	from	database	1230:1237	arg1	14732T					1323:1328	Aeromicrobium ginsengisoli JCM 14732T	1292:1328	Aeromicrobium ginsengisoli JCM 14732T	1292:1328	The four strains' genomes displayed less than 24.6 % DNA-DNA relatedness with all available genomes of the genus Aeromicrobium in the NCBI database, including Aeromicrobium fastidiosum NBRC 14897T and Aeromicrobium ginsengisoli JCM 14732T.
32692642	9	27	from	database	1230:1237	arg1	14897T					1281:1286	Aeromicrobium fastidiosum NBRC 14897T	1250:1286	Aeromicrobium fastidiosum NBRC 14897T	1250:1286	The four strains' genomes displayed less than 24.6 % DNA-DNA relatedness with all available genomes of the genus Aeromicrobium in the NCBI database, including Aeromicrobium fastidiosum NBRC 14897T and Aeromicrobium ginsengisoli JCM 14732T.
32692642	9	27	from	database	1230:1237	arg1	genomes					1183:1189	all available genomes	1169:1189	all available genomes	1169:1189	The four strains' genomes displayed less than 24.6 % DNA-DNA relatedness with all available genomes of the genus Aeromicrobium in the NCBI database, including Aeromicrobium fastidiosum NBRC 14897T and Aeromicrobium ginsengisoli JCM 14732T.
32692642	16	28	theme	1.17444T=JCM	1933:1944	arg1	MF47T					1919:1923	MF47T	1919:1923	MF47T (=CGMCC 1.17444T=JCM 33790T)	1919:1952	The type strains are 592T (=CGMCC1.16526T=DSM 106289T) and MF47T (=CGMCC 1.17444T=JCM 33790T), respectively.
32692642	16	28	theme	1.17444T=JCM	1933:1944	arg1	33790T					1946:1951	=CGMCC 1.17444T=JCM 33790T	1926:1951	=CGMCC 1.17444T=JCM 33790T	1926:1951	The type strains are 592T (=CGMCC1.16526T=DSM 106289T) and MF47T (=CGMCC 1.17444T=JCM 33790T), respectively.
32692642	7	29	theme	DNA	847:849	arg1	contents					855:862	The DNA G+C contents	843:862	The DNA G+C contents of strains 592T and MF47T	843:888	The DNA G+C contents of strains 592T and MF47T were 70.3 and 69.8 mol%, respectively.
32692642	7	29	theme	DNA	847:849	arg1	%					912:912	70.3 and 69.8 mol%	895:912	70.3 and 69.8 mol%	895:912	The DNA G+C contents of strains 592T and MF47T were 70.3 and 69.8 mol%, respectively.
32692642	3	30	theme	plateau	289:295	arg1	pikas					297:301	plateau pikas	289:301	plateau pikas (Ochotona curzoniae)	289:322	Four novel strains (592T, S592, MF47T and SMF47) were isolated from Tibetan antelopes (Pantholops hodgsonii) and plateau pikas (Ochotona curzoniae), respectively.
32692642	3	30	theme	plateau	289:295	arg1	Ochotona					304:311	Ochotona	304:311	Ochotona	304:311	Four novel strains (592T, S592, MF47T and SMF47) were isolated from Tibetan antelopes (Pantholops hodgsonii) and plateau pikas (Ochotona curzoniae), respectively.
32692642	5	31	theme	genomic	649:655	arg1	sequences					657:665	genomic sequences	649:665	genomic sequences	649:665	The 16S rRNA gene sequences of the four strains showed highest similarities to Aeromicrobium fastidiosum DSM 10552T (98.1, 98.6, 98.7 and 98.7 %, respectively), and the phylogenetic analyses based on 16S rRNA gene and genomic sequences indicated that strains 592T and MF47T represent two novel species.
32692642	9	32	theme	available	1173:1181	arg1	14732T					1323:1328	Aeromicrobium ginsengisoli JCM 14732T	1292:1328	Aeromicrobium ginsengisoli JCM 14732T	1292:1328	The four strains' genomes displayed less than 24.6 % DNA-DNA relatedness with all available genomes of the genus Aeromicrobium in the NCBI database, including Aeromicrobium fastidiosum NBRC 14897T and Aeromicrobium ginsengisoli JCM 14732T.
32692642	9	32	theme	available	1173:1181	arg1	14897T					1281:1286	Aeromicrobium fastidiosum NBRC 14897T	1250:1286	Aeromicrobium fastidiosum NBRC 14897T	1250:1286	The four strains' genomes displayed less than 24.6 % DNA-DNA relatedness with all available genomes of the genus Aeromicrobium in the NCBI database, including Aeromicrobium fastidiosum NBRC 14897T and Aeromicrobium ginsengisoli JCM 14732T.
32692642	9	32	theme	available	1173:1181	arg1	genomes					1183:1189	all available genomes	1169:1189	all available genomes	1169:1189	The four strains' genomes displayed less than 24.6 % DNA-DNA relatedness with all available genomes of the genus Aeromicrobium in the NCBI database, including Aeromicrobium fastidiosum NBRC 14897T and Aeromicrobium ginsengisoli JCM 14732T.
32692642	8	33	theme	digital	933:939	arg1	hybridization					949:961	digital DNA-DNA hybridization	933:961	The digital DNA-DNA hybridization value between strains 592T and MF47T	929:998	The digital DNA-DNA hybridization value between strains 592T and MF47T was 32.6 %, lower than the threshold of 70 %, indicating they belong to different species.
32692642	5	34	theme	Aeromicrobium	510:522	arg1	10552T					540:545	Aeromicrobium fastidiosum DSM 10552T	510:545	Aeromicrobium fastidiosum DSM 10552T	510:545	The 16S rRNA gene sequences of the four strains showed highest similarities to Aeromicrobium fastidiosum DSM 10552T (98.1, 98.6, 98.7 and 98.7 %, respectively), and the phylogenetic analyses based on 16S rRNA gene and genomic sequences indicated that strains 592T and MF47T represent two novel species.
32692642	10	35	theme	major	1335:1339	arg1	C18 					1378:1381	C18 	1378:1381	C18 	1378:1381	The major fatty acids of the four strains were C18 : 1 ω9c and C18 : 0 10-methyl, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol.
32692642	10	35	theme	major	1335:1339	arg1	acids					1347:1351	The major fatty acids	1331:1351	The major fatty acids of the four strains	1331:1371	The major fatty acids of the four strains were C18 : 1 ω9c and C18 : 0 10-methyl, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol.
32692642	3	36	theme	novel	181:185	arg1	strains					187:193	Four novel strains	176:193	Four novel strains (592T, S592, MF47T and SMF47)	176:223	Four novel strains (592T, S592, MF47T and SMF47) were isolated from Tibetan antelopes (Pantholops hodgsonii) and plateau pikas (Ochotona curzoniae), respectively.
32692642	3	36	theme	novel	181:185	arg1	MF47T					208:212	MF47T	208:212	MF47T	208:212	Four novel strains (592T, S592, MF47T and SMF47) were isolated from Tibetan antelopes (Pantholops hodgsonii) and plateau pikas (Ochotona curzoniae), respectively.
32692642	3	36	theme	novel	181:185	arg1	SMF47					218:222	SMF47	218:222	SMF47	218:222	Four novel strains (592T, S592, MF47T and SMF47) were isolated from Tibetan antelopes (Pantholops hodgsonii) and plateau pikas (Ochotona curzoniae), respectively.
32692642	3	36	theme	novel	181:185	arg1	S592					202:205	S592	202:205	S592	202:205	Four novel strains (592T, S592, MF47T and SMF47) were isolated from Tibetan antelopes (Pantholops hodgsonii) and plateau pikas (Ochotona curzoniae), respectively.
32692642	5	37	theme	novel	719:723	arg1	species					725:731	two novel species	715:731	two novel species	715:731	The 16S rRNA gene sequences of the four strains showed highest similarities to Aeromicrobium fastidiosum DSM 10552T (98.1, 98.6, 98.7 and 98.7 %, respectively), and the phylogenetic analyses based on 16S rRNA gene and genomic sequences indicated that strains 592T and MF47T represent two novel species.
32692642	8	38	theme	hybridization	949:961	arg1	%					1009:1009	32.6 %	1004:1009	32.6 %	1004:1009	The digital DNA-DNA hybridization value between strains 592T and MF47T was 32.6 %, lower than the threshold of 70 %, indicating they belong to different species.
32692642	8	38	theme	hybridization	949:961	arg1	value					963:967	The digital DNA-DNA hybridization value	929:967	The digital DNA-DNA hybridization value between strains 592T and MF47T	929:998	The digital DNA-DNA hybridization value between strains 592T and MF47T was 32.6 %, lower than the threshold of 70 %, indicating they belong to different species.
32692642	5	39	theme	DSM	536:538	arg1	10552T					540:545	Aeromicrobium fastidiosum DSM 10552T	510:545	Aeromicrobium fastidiosum DSM 10552T	510:545	The 16S rRNA gene sequences of the four strains showed highest similarities to Aeromicrobium fastidiosum DSM 10552T (98.1, 98.6, 98.7 and 98.7 %, respectively), and the phylogenetic analyses based on 16S rRNA gene and genomic sequences indicated that strains 592T and MF47T represent two novel species.
32692642	9	40	dep	relatedness	1152:1162	arg1	%					1142:1142	%	1142:1142	%	1142:1142	The four strains' genomes displayed less than 24.6 % DNA-DNA relatedness with all available genomes of the genus Aeromicrobium in the NCBI database, including Aeromicrobium fastidiosum NBRC 14897T and Aeromicrobium ginsengisoli JCM 14732T.
32692642	3	41	dep	Pantholops	263:272	arg1	hodgsonii					274:282	Pantholops hodgsonii	263:282	Pantholops hodgsonii	263:282	Four novel strains (592T, S592, MF47T and SMF47) were isolated from Tibetan antelopes (Pantholops hodgsonii) and plateau pikas (Ochotona curzoniae), respectively.
32692642	9	42	theme	genus	1198:1202	arg1	Aeromicrobium					1204:1216	the genus Aeromicrobium	1194:1216	the genus Aeromicrobium in the NCBI database	1194:1237	The four strains' genomes displayed less than 24.6 % DNA-DNA relatedness with all available genomes of the genus Aeromicrobium in the NCBI database, including Aeromicrobium fastidiosum NBRC 14897T and Aeromicrobium ginsengisoli JCM 14732T.
32692642	9	43	dep	%	1142:1142	arg1	24.6 					1137:1141	24.6 	1137:1141	24.6 	1137:1141	The four strains' genomes displayed less than 24.6 % DNA-DNA relatedness with all available genomes of the genus Aeromicrobium in the NCBI database, including Aeromicrobium fastidiosum NBRC 14897T and Aeromicrobium ginsengisoli JCM 14732T.
32692642	11	44	theme	predominant	1519:1529	arg1	MK-9					1557:1560	MK-9	1557:1560	MK-9	1557:1560	The predominant respiratory quinones were MK-9(H4) and MK-8(H4).
32692642	11	44	theme	predominant	1519:1529	arg1	quinones					1543:1550	The predominant respiratory quinones	1515:1550	The predominant respiratory quinones	1515:1550	The predominant respiratory quinones were MK-9(H4) and MK-8(H4).
32692642	9	45	theme	NCBI	1225:1228	arg1	database					1230:1237	the NCBI database	1221:1237	the NCBI database	1221:1237	The four strains' genomes displayed less than 24.6 % DNA-DNA relatedness with all available genomes of the genus Aeromicrobium in the NCBI database, including Aeromicrobium fastidiosum NBRC 14897T and Aeromicrobium ginsengisoli JCM 14732T.
32692642	13	46	theme	genotypic	1657:1665	arg1	analyses					1695:1702	these genotypic, phenotypic and biochemical analyses	1651:1702	these genotypic, phenotypic and biochemical analyses	1651:1702	Based on these genotypic, phenotypic and biochemical analyses, it is proposed that the four unidentified bacteria be classified as two novel species, Aeromicrobium chenweiae sp.
32692642	9	47	theme	Aeromicrobium	1250:1262	arg1	14897T					1281:1286	Aeromicrobium fastidiosum NBRC 14897T	1250:1286	Aeromicrobium fastidiosum NBRC 14897T	1250:1286	The four strains' genomes displayed less than 24.6 % DNA-DNA relatedness with all available genomes of the genus Aeromicrobium in the NCBI database, including Aeromicrobium fastidiosum NBRC 14897T and Aeromicrobium ginsengisoli JCM 14732T.
32692642	1	48	theme	yanjiei	51:57	arg1	sp					59:60	yanjiei sp	51:60	yanjiei sp	51:60	nov. and Aeromicrobium yanjiei sp.
32692642	13	49	theme	phenotypic	1668:1677	arg1	analyses					1695:1702	these genotypic, phenotypic and biochemical analyses	1651:1702	these genotypic, phenotypic and biochemical analyses	1651:1702	Based on these genotypic, phenotypic and biochemical analyses, it is proposed that the four unidentified bacteria be classified as two novel species, Aeromicrobium chenweiae sp.
32692642	9	50	theme	NBRC	1276:1279	arg1	14897T					1281:1286	Aeromicrobium fastidiosum NBRC 14897T	1250:1286	Aeromicrobium fastidiosum NBRC 14897T	1250:1286	The four strains' genomes displayed less than 24.6 % DNA-DNA relatedness with all available genomes of the genus Aeromicrobium in the NCBI database, including Aeromicrobium fastidiosum NBRC 14897T and Aeromicrobium ginsengisoli JCM 14732T.
32692642	2	51	dep	Ochotona	141:148	arg1	curzoniae					150:158	Ochotona curzoniae	141:158	Ochotona curzoniae	141:158	nov., isolated from Tibetan antelope (Pantholops hodgsonii) and plateau pika (Ochotona curzoniae), respectively.
32692642	5	52	theme	rRNA	439:442	arg1	sequences					449:457	The 16S rRNA gene sequences	431:457	The 16S rRNA gene sequences of the four strains	431:477	The 16S rRNA gene sequences of the four strains showed highest similarities to Aeromicrobium fastidiosum DSM 10552T (98.1, 98.6, 98.7 and 98.7 %, respectively), and the phylogenetic analyses based on 16S rRNA gene and genomic sequences indicated that strains 592T and MF47T represent two novel species.
32692642	9	53	theme	ginsengisoli	1306:1317	arg1	14732T					1323:1328	Aeromicrobium ginsengisoli JCM 14732T	1292:1328	Aeromicrobium ginsengisoli JCM 14732T	1292:1328	The four strains' genomes displayed less than 24.6 % DNA-DNA relatedness with all available genomes of the genus Aeromicrobium in the NCBI database, including Aeromicrobium fastidiosum NBRC 14897T and Aeromicrobium ginsengisoli JCM 14732T.
32692642	5	54	dep	showed	479:484	arg1	%					574:574	98.1, 98.6, 98.7 and 98.7 %	548:574	98.1, 98.6, 98.7 and 98.7 %	548:574	The 16S rRNA gene sequences of the four strains showed highest similarities to Aeromicrobium fastidiosum DSM 10552T (98.1, 98.6, 98.7 and 98.7 %, respectively), and the phylogenetic analyses based on 16S rRNA gene and genomic sequences indicated that strains 592T and MF47T represent two novel species.
32692642	3	55	dep	strains	187:193	arg1	SMF47					218:222	SMF47	218:222	SMF47	218:222	Four novel strains (592T, S592, MF47T and SMF47) were isolated from Tibetan antelopes (Pantholops hodgsonii) and plateau pikas (Ochotona curzoniae), respectively.
32692642	3	55	dep	strains	187:193	arg1	MF47T					208:212	MF47T	208:212	MF47T	208:212	Four novel strains (592T, S592, MF47T and SMF47) were isolated from Tibetan antelopes (Pantholops hodgsonii) and plateau pikas (Ochotona curzoniae), respectively.
32692642	3	55	dep	strains	187:193	arg1	strains					187:193	Four novel strains	176:193	Four novel strains (592T, S592, MF47T and SMF47)	176:223	Four novel strains (592T, S592, MF47T and SMF47) were isolated from Tibetan antelopes (Pantholops hodgsonii) and plateau pikas (Ochotona curzoniae), respectively.
32692642	3	55	dep	strains	187:193	arg1	S592					202:205	S592	202:205	S592	202:205	Four novel strains (592T, S592, MF47T and SMF47) were isolated from Tibetan antelopes (Pantholops hodgsonii) and plateau pikas (Ochotona curzoniae), respectively.
32692642	11	56	theme	respiratory	1531:1541	arg1	MK-9					1557:1560	MK-9	1557:1560	MK-9	1557:1560	The predominant respiratory quinones were MK-9(H4) and MK-8(H4).
32692642	11	56	theme	respiratory	1531:1541	arg1	quinones					1543:1550	The predominant respiratory quinones	1515:1550	The predominant respiratory quinones	1515:1550	The predominant respiratory quinones were MK-9(H4) and MK-8(H4).
32692642	9	57	from	genomes	1183:1189	arg1	database					1230:1237	the NCBI database	1221:1237	the NCBI database	1221:1237	The four strains' genomes displayed less than 24.6 % DNA-DNA relatedness with all available genomes of the genus Aeromicrobium in the NCBI database, including Aeromicrobium fastidiosum NBRC 14897T and Aeromicrobium ginsengisoli JCM 14732T.
32692642	12	58	theme	cell-wall	1584:1592	arg1	peptidoglycan					1594:1606	The cell-wall peptidoglycan	1580:1606	The cell-wall peptidoglycan	1580:1606	The cell-wall peptidoglycan contained ll-diaminopimelic acid.
32692642	10	59	theme	main	1421:1424	arg1	diphosphatidylglycerol					1444:1465	diphosphatidylglycerol	1444:1465	diphosphatidylglycerol	1444:1465	The major fatty acids of the four strains were C18 : 1 ω9c and C18 : 0 10-methyl, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol.
32692642	10	59	theme	main	1421:1424	arg1	lipids					1432:1437	the main polar lipids	1417:1437	the main polar lipids	1417:1437	The major fatty acids of the four strains were C18 : 1 ω9c and C18 : 0 10-methyl, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol.
32692642	13	60	theme	chenweiae	1806:1814	arg1	species					1783:1789	two novel species	1773:1789	two novel species	1773:1789	Based on these genotypic, phenotypic and biochemical analyses, it is proposed that the four unidentified bacteria be classified as two novel species, Aeromicrobium chenweiae sp.
32692642	13	60	theme	chenweiae	1806:1814	arg1	sp					1816:1817	Aeromicrobium chenweiae sp	1792:1817	Aeromicrobium chenweiae sp	1792:1817	Based on these genotypic, phenotypic and biochemical analyses, it is proposed that the four unidentified bacteria be classified as two novel species, Aeromicrobium chenweiae sp.
32692642	5	61	dep	strains	682:688	arg1	MF47T					699:703	MF47T	699:703	MF47T	699:703	The 16S rRNA gene sequences of the four strains showed highest similarities to Aeromicrobium fastidiosum DSM 10552T (98.1, 98.6, 98.7 and 98.7 %, respectively), and the phylogenetic analyses based on 16S rRNA gene and genomic sequences indicated that strains 592T and MF47T represent two novel species.
32692642	5	61	dep	strains	682:688	arg1	592T					690:693	592T	690:693	592T	690:693	The 16S rRNA gene sequences of the four strains showed highest similarities to Aeromicrobium fastidiosum DSM 10552T (98.1, 98.6, 98.7 and 98.7 %, respectively), and the phylogenetic analyses based on 16S rRNA gene and genomic sequences indicated that strains 592T and MF47T represent two novel species.
32692642	5	61	dep	strains	682:688	arg1	strains					682:688	strains	682:688	strains 592T and MF47T	682:703	The 16S rRNA gene sequences of the four strains showed highest similarities to Aeromicrobium fastidiosum DSM 10552T (98.1, 98.6, 98.7 and 98.7 %, respectively), and the phylogenetic analyses based on 16S rRNA gene and genomic sequences indicated that strains 592T and MF47T represent two novel species.
32692642	9	62	dep	Aeromicrobium	1250:1262	arg1	fastidiosum					1264:1274	fastidiosum	1264:1274	fastidiosum	1264:1274	The four strains' genomes displayed less than 24.6 % DNA-DNA relatedness with all available genomes of the genus Aeromicrobium in the NCBI database, including Aeromicrobium fastidiosum NBRC 14897T and Aeromicrobium ginsengisoli JCM 14732T.
32692642	8	63	dep	strains	977:983	arg1	592T					985:988	592T	985:988	592T	985:988	The digital DNA-DNA hybridization value between strains 592T and MF47T was 32.6 %, lower than the threshold of 70 %, indicating they belong to different species.
32692642	8	63	dep	strains	977:983	arg1	strains					977:983	strains 592T and MF47T	977:998	strains 592T and MF47T	977:998	The digital DNA-DNA hybridization value between strains 592T and MF47T was 32.6 %, lower than the threshold of 70 %, indicating they belong to different species.
32692642	8	63	dep	strains	977:983	arg1	MF47T					994:998	MF47T	994:998	MF47T	994:998	The digital DNA-DNA hybridization value between strains 592T and MF47T was 32.6 %, lower than the threshold of 70 %, indicating they belong to different species.
32692642	2	64	theme	Tibetan	83:89	arg1	hodgsonii					112:120	Pantholops hodgsonii	101:120	Pantholops hodgsonii	101:120	nov., isolated from Tibetan antelope (Pantholops hodgsonii) and plateau pika (Ochotona curzoniae), respectively.
32692642	2	64	theme	Tibetan	83:89	arg1	antelope					91:98	Tibetan antelope	83:98	Tibetan antelope (Pantholops hodgsonii)	83:121	nov., isolated from Tibetan antelope (Pantholops hodgsonii) and plateau pika (Ochotona curzoniae), respectively.
32692642	5	65	theme	highest	486:492	arg1	similarities					494:505	highest similarities	486:505	highest similarities to Aeromicrobium fastidiosum DSM 10552T	486:545	The 16S rRNA gene sequences of the four strains showed highest similarities to Aeromicrobium fastidiosum DSM 10552T (98.1, 98.6, 98.7 and 98.7 %, respectively), and the phylogenetic analyses based on 16S rRNA gene and genomic sequences indicated that strains 592T and MF47T represent two novel species.
32692642	7	66	theme	70.3 and	895:902	arg1	contents					855:862	The DNA G+C contents	843:862	The DNA G+C contents of strains 592T and MF47T	843:888	The DNA G+C contents of strains 592T and MF47T were 70.3 and 69.8 mol%, respectively.
32692642	7	66	theme	70.3 and	895:902	arg1	%					912:912	70.3 and 69.8 mol%	895:912	70.3 and 69.8 mol%	895:912	The DNA G+C contents of strains 592T and MF47T were 70.3 and 69.8 mol%, respectively.
32692642	3	67	theme	Tibetan	244:250	arg1	Pantholops					263:272	Pantholops	263:272	Pantholops	263:272	Four novel strains (592T, S592, MF47T and SMF47) were isolated from Tibetan antelopes (Pantholops hodgsonii) and plateau pikas (Ochotona curzoniae), respectively.
32692642	3	67	theme	Tibetan	244:250	arg1	antelopes					252:260	Tibetan antelopes	244:260	Tibetan antelopes (Pantholops hodgsonii)	244:283	Four novel strains (592T, S592, MF47T and SMF47) were isolated from Tibetan antelopes (Pantholops hodgsonii) and plateau pikas (Ochotona curzoniae), respectively.
32692642	10	68	dep	C18 	1378:1381	arg1	C18 					1394:1397	C18 	1394:1397	C18 	1394:1397	The major fatty acids of the four strains were C18 : 1 ω9c and C18 : 0 10-methyl, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol.
32692642	10	68	dep	C18 	1378:1381	arg1	10-methyl					1402:1410	 0 10-methyl	1399:1410	C18 : 1 ω9c and C18 : 0 10-methyl	1378:1410	The major fatty acids of the four strains were C18 : 1 ω9c and C18 : 0 10-methyl, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol.
32692642	10	68	dep	C18 	1378:1381	arg1	ω9c					1386:1388	ω9c	1386:1388	ω9c	1386:1388	The major fatty acids of the four strains were C18 : 1 ω9c and C18 : 0 10-methyl, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol.
32692642	5	69	theme	strains	471:477	arg1	sequences					449:457	The 16S rRNA gene sequences	431:457	The 16S rRNA gene sequences of the four strains	431:477	The 16S rRNA gene sequences of the four strains showed highest similarities to Aeromicrobium fastidiosum DSM 10552T (98.1, 98.6, 98.7 and 98.7 %, respectively), and the phylogenetic analyses based on 16S rRNA gene and genomic sequences indicated that strains 592T and MF47T represent two novel species.
32692642	5	70	theme	rRNA	635:638	arg1	gene					640:643	16S rRNA gene	631:643	16S rRNA gene	631:643	The 16S rRNA gene sequences of the four strains showed highest similarities to Aeromicrobium fastidiosum DSM 10552T (98.1, 98.6, 98.7 and 98.7 %, respectively), and the phylogenetic analyses based on 16S rRNA gene and genomic sequences indicated that strains 592T and MF47T represent two novel species.
32692642	16	71	theme	=CGMCC	1926:1931	arg1	MF47T					1919:1923	MF47T	1919:1923	MF47T (=CGMCC 1.17444T=JCM 33790T)	1919:1952	The type strains are 592T (=CGMCC1.16526T=DSM 106289T) and MF47T (=CGMCC 1.17444T=JCM 33790T), respectively.
32692642	16	71	theme	=CGMCC	1926:1931	arg1	33790T					1946:1951	=CGMCC 1.17444T=JCM 33790T	1926:1951	=CGMCC 1.17444T=JCM 33790T	1926:1951	The type strains are 592T (=CGMCC1.16526T=DSM 106289T) and MF47T (=CGMCC 1.17444T=JCM 33790T), respectively.
32692642	0	72	theme	Aeromicrobium	0:12	arg1	sp					24:25	Aeromicrobium chenweiae sp	0:25	Aeromicrobium chenweiae sp.	0:26	Aeromicrobium chenweiae sp.
32692642	12	73	contain	contained	1608:1616	arg2	acid					1636:1639	ll-diaminopimelic acid	1618:1639	ll-diaminopimelic acid	1618:1639	The cell-wall peptidoglycan contained ll-diaminopimelic acid.
32692642	12	73	contain	contained	1608:1616	arg1	peptidoglycan					1594:1606	The cell-wall peptidoglycan	1580:1606	The cell-wall peptidoglycan	1580:1606	The cell-wall peptidoglycan contained ll-diaminopimelic acid.
32692642	8	74	theme	DNA-DNA	941:947	arg1	hybridization					949:961	digital DNA-DNA hybridization	933:961	The digital DNA-DNA hybridization value between strains 592T and MF47T	929:998	The digital DNA-DNA hybridization value between strains 592T and MF47T was 32.6 %, lower than the threshold of 70 %, indicating they belong to different species.
32692642	7	75	theme	G+C	851:853	arg1	contents					855:862	The DNA G+C contents	843:862	The DNA G+C contents of strains 592T and MF47T	843:888	The DNA G+C contents of strains 592T and MF47T were 70.3 and 69.8 mol%, respectively.
32692642	7	75	theme	G+C	851:853	arg1	%					912:912	70.3 and 69.8 mol%	895:912	70.3 and 69.8 mol%	895:912	The DNA G+C contents of strains 592T and MF47T were 70.3 and 69.8 mol%, respectively.
32692642	10	76	theme	fatty	1341:1345	arg1	C18 					1378:1381	C18 	1378:1381	C18 	1378:1381	The major fatty acids of the four strains were C18 : 1 ω9c and C18 : 0 10-methyl, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol.
32692642	10	76	theme	fatty	1341:1345	arg1	acids					1347:1351	The major fatty acids	1331:1351	The major fatty acids of the four strains	1331:1371	The major fatty acids of the four strains were C18 : 1 ω9c and C18 : 0 10-methyl, and the main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol.
32692642	0	77	theme	chenweiae	14:22	arg1	sp					24:25	Aeromicrobium chenweiae sp	0:25	Aeromicrobium chenweiae sp.	0:26	Aeromicrobium chenweiae sp.
32692642	5	78	theme	fastidiosum	524:534	arg1	10552T					540:545	Aeromicrobium fastidiosum DSM 10552T	510:545	Aeromicrobium fastidiosum DSM 10552T	510:545	The 16S rRNA gene sequences of the four strains showed highest similarities to Aeromicrobium fastidiosum DSM 10552T (98.1, 98.6, 98.7 and 98.7 %, respectively), and the phylogenetic analyses based on 16S rRNA gene and genomic sequences indicated that strains 592T and MF47T represent two novel species.
32692642	7	79	dep	strains	867:873	arg1	strains					867:873	strains 592T and MF47T	867:888	strains 592T and MF47T	867:888	The DNA G+C contents of strains 592T and MF47T were 70.3 and 69.8 mol%, respectively.
32692642	7	79	dep	strains	867:873	arg1	MF47T					884:888	MF47T	884:888	MF47T	884:888	The DNA G+C contents of strains 592T and MF47T were 70.3 and 69.8 mol%, respectively.
32692642	7	79	dep	strains	867:873	arg1	592T					875:878	592T	875:878	592T	875:878	The DNA G+C contents of strains 592T and MF47T were 70.3 and 69.8 mol%, respectively.
32692642	14	80	theme	yanjiei	1843:1849	arg1	sp					1851:1852	yanjiei sp	1843:1852	yanjiei sp	1843:1852	nov. and Aeromicrobium yanjiei sp.
32692642	9	81	theme	Aeromicrobium	1204:1216	arg1	14732T					1323:1328	Aeromicrobium ginsengisoli JCM 14732T	1292:1328	Aeromicrobium ginsengisoli JCM 14732T	1292:1328	The four strains' genomes displayed less than 24.6 % DNA-DNA relatedness with all available genomes of the genus Aeromicrobium in the NCBI database, including Aeromicrobium fastidiosum NBRC 14897T and Aeromicrobium ginsengisoli JCM 14732T.
32692642	9	81	theme	Aeromicrobium	1204:1216	arg1	14897T					1281:1286	Aeromicrobium fastidiosum NBRC 14897T	1250:1286	Aeromicrobium fastidiosum NBRC 14897T	1250:1286	The four strains' genomes displayed less than 24.6 % DNA-DNA relatedness with all available genomes of the genus Aeromicrobium in the NCBI database, including Aeromicrobium fastidiosum NBRC 14897T and Aeromicrobium ginsengisoli JCM 14732T.
32692642	9	81	theme	Aeromicrobium	1204:1216	arg1	genomes					1183:1189	all available genomes	1169:1189	all available genomes	1169:1189	The four strains' genomes displayed less than 24.6 % DNA-DNA relatedness with all available genomes of the genus Aeromicrobium in the NCBI database, including Aeromicrobium fastidiosum NBRC 14897T and Aeromicrobium ginsengisoli JCM 14732T.
32692642	3	82	attach	isolated	230:237	arg2	SMF47					218:222	SMF47	218:222	SMF47	218:222	Four novel strains (592T, S592, MF47T and SMF47) were isolated from Tibetan antelopes (Pantholops hodgsonii) and plateau pikas (Ochotona curzoniae), respectively.
32692642	3	82	attach	isolated	230:237	arg1	antelopes					252:260	Tibetan antelopes	244:260	Tibetan antelopes (Pantholops hodgsonii)	244:283	Four novel strains (592T, S592, MF47T and SMF47) were isolated from Tibetan antelopes (Pantholops hodgsonii) and plateau pikas (Ochotona curzoniae), respectively.
32692642	3	82	attach	isolated	230:237	arg2	S592					202:205	S592	202:205	S592	202:205	Four novel strains (592T, S592, MF47T and SMF47) were isolated from Tibetan antelopes (Pantholops hodgsonii) and plateau pikas (Ochotona curzoniae), respectively.
32692642	3	82	attach	isolated	230:237	arg1	pikas					297:301	plateau pikas	289:301	plateau pikas (Ochotona curzoniae)	289:322	Four novel strains (592T, S592, MF47T and SMF47) were isolated from Tibetan antelopes (Pantholops hodgsonii) and plateau pikas (Ochotona curzoniae), respectively.
32692642	3	82	attach	isolated	230:237	arg2	MF47T					208:212	MF47T	208:212	MF47T	208:212	Four novel strains (592T, S592, MF47T and SMF47) were isolated from Tibetan antelopes (Pantholops hodgsonii) and plateau pikas (Ochotona curzoniae), respectively.
32692642	3	82	attach	isolated	230:237	arg1	Ochotona					304:311	Ochotona	304:311	Ochotona	304:311	Four novel strains (592T, S592, MF47T and SMF47) were isolated from Tibetan antelopes (Pantholops hodgsonii) and plateau pikas (Ochotona curzoniae), respectively.
32692642	3	82	attach	isolated	230:237	arg1	Pantholops					263:272	Pantholops	263:272	Pantholops	263:272	Four novel strains (592T, S592, MF47T and SMF47) were isolated from Tibetan antelopes (Pantholops hodgsonii) and plateau pikas (Ochotona curzoniae), respectively.
32692642	3	82	attach	isolated	230:237	arg2	strains					187:193	Four novel strains	176:193	Four novel strains (592T, S592, MF47T and SMF47)	176:223	Four novel strains (592T, S592, MF47T and SMF47) were isolated from Tibetan antelopes (Pantholops hodgsonii) and plateau pikas (Ochotona curzoniae), respectively.
32692642	16	83	theme	=CGMCC1.16526T=DSM	1887:1904	arg1	592T					1881:1884	592T	1881:1884	592T (=CGMCC1.16526T=DSM 106289T)	1881:1913	The type strains are 592T (=CGMCC1.16526T=DSM 106289T) and MF47T (=CGMCC 1.17444T=JCM 33790T), respectively.
32692642	16	83	theme	=CGMCC1.16526T=DSM	1887:1904	arg1	106289T					1906:1912	=CGMCC1.16526T=DSM 106289T	1887:1912	=CGMCC1.16526T=DSM 106289T	1887:1912	The type strains are 592T (=CGMCC1.16526T=DSM 106289T) and MF47T (=CGMCC 1.17444T=JCM 33790T), respectively.
32692642	9	84	from	Aeromicrobium	1204:1216	arg1	database					1230:1237	the NCBI database	1221:1237	the NCBI database	1221:1237	The four strains' genomes displayed less than 24.6 % DNA-DNA relatedness with all available genomes of the genus Aeromicrobium in the NCBI database, including Aeromicrobium fastidiosum NBRC 14897T and Aeromicrobium ginsengisoli JCM 14732T.
32692642	9	85	dep	strains	1100:1106	arg1	genomes					1109:1115	genomes	1109:1115	The four strains' genomes	1091:1115	The four strains' genomes displayed less than 24.6 % DNA-DNA relatedness with all available genomes of the genus Aeromicrobium in the NCBI database, including Aeromicrobium fastidiosum NBRC 14897T and Aeromicrobium ginsengisoli JCM 14732T.
32692642	3	86	dep	Ochotona	304:311	arg1	curzoniae					313:321	Ochotona curzoniae	304:321	Ochotona curzoniae	304:321	Four novel strains (592T, S592, MF47T and SMF47) were isolated from Tibetan antelopes (Pantholops hodgsonii) and plateau pikas (Ochotona curzoniae), respectively.
32692642	8	87	theme	lower	1012:1016	arg1	value					963:967	The digital DNA-DNA hybridization value	929:967	The digital DNA-DNA hybridization value between strains 592T and MF47T	929:998	The digital DNA-DNA hybridization value between strains 592T and MF47T was 32.6 %, lower than the threshold of 70 %, indicating they belong to different species.
32692642	8	87	theme	lower	1012:1016	arg1	%					1009:1009	32.6 %	1004:1009	32.6 %	1004:1009	The digital DNA-DNA hybridization value between strains 592T and MF47T was 32.6 %, lower than the threshold of 70 %, indicating they belong to different species.
31746729	8	0	theme	strain	1182:1187	arg1	K30003T					1194:1200	strain SYSU K30003T	1182:1200	strain SYSU K30003T	1182:1200	The major fatty acids (>5 %) of strain SYSU K30003T were anteiso-C15:0, C16 : 0, anteiso-C17 : 0 and iso-C16 : 0, while those of strain SYSU K30004T were anteiso-C15:0, C16:0, anteiso-C17 : 0, iso-C15 : 0, iso-C16:0 and iso-C17 : 0.
31746729	6	1	theme	K30004T	783:789	arg1	profile					760:766	The polar lipid profile	744:766	The polar lipid profile of strain SYSU K30004T	744:789	The polar lipid profile of strain SYSU K30004T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two aminophospholipids, an unidentified glycolipid, unidentified phospholipids and two unidentified polar lipids.
31746729	8	2	theme	K30003T	1194:1200	arg1	acids					1166:1170	The major fatty acids	1150:1170	The major fatty acids (>5 %) of strain SYSU K30003T	1150:1200	The major fatty acids (>5 %) of strain SYSU K30003T were anteiso-C15:0, C16 : 0, anteiso-C17 : 0 and iso-C16 : 0, while those of strain SYSU K30004T were anteiso-C15:0, C16:0, anteiso-C17 : 0, iso-C15 : 0, iso-C16:0 and iso-C17 : 0.
31746729	8	2	theme	K30003T	1194:1200	arg1	%					1176:1176	>5 %	1173:1176	>5 %	1173:1176	The major fatty acids (>5 %) of strain SYSU K30003T were anteiso-C15:0, C16 : 0, anteiso-C17 : 0 and iso-C16 : 0, while those of strain SYSU K30004T were anteiso-C15:0, C16:0, anteiso-C17 : 0, iso-C15 : 0, iso-C16:0 and iso-C17 : 0.
31746729	8	2	theme	K30003T	1194:1200	arg1	anteiso-C15:0					1207:1219	anteiso-C15:0	1207:1219	anteiso-C15:0	1207:1219	The major fatty acids (>5 %) of strain SYSU K30003T were anteiso-C15:0, C16 : 0, anteiso-C17 : 0 and iso-C16 : 0, while those of strain SYSU K30004T were anteiso-C15:0, C16:0, anteiso-C17 : 0, iso-C15 : 0, iso-C16:0 and iso-C17 : 0.
31746729	6	3	theme	unidentified	903:914	arg1	glycolipid					916:925	an unidentified glycolipid	900:925	an unidentified glycolipid	900:925	The polar lipid profile of strain SYSU K30004T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two aminophospholipids, an unidentified glycolipid, unidentified phospholipids and two unidentified polar lipids.
31746729	6	4	theme	strain	771:776	arg1	K30004T					783:789	strain SYSU K30004T	771:789	strain SYSU K30004T	771:789	The polar lipid profile of strain SYSU K30004T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two aminophospholipids, an unidentified glycolipid, unidentified phospholipids and two unidentified polar lipids.
31746729	11	5	theme	novel	1811:1815	arg1	species					1817:1823	two novel species	1807:1823	two novel species	1807:1823	Based on the results of phenotypic, chemotaxonomic and genome analyses, strains SYSU K30003T and SYSU K30004T represent two novel species of the genus Paenibacillus, for which the names Paenibacillus antri sp.
31746729	9	6	theme	genome	1387:1392	arg1	%					1470:1470	59.0 and 53.6 mol%	1453:1470	59.0 and 53.6 mol%	1453:1470	The genome G+C contents of strains SYSU K30003T and SYSU K30004T were 59.0 and 53.6 mol%, respectively.
31746729	9	6	theme	genome	1387:1392	arg1	contents					1398:1405	The genome G+C contents	1383:1405	The genome G+C contents of strains SYSU K30003T and SYSU K30004T	1383:1446	The genome G+C contents of strains SYSU K30003T and SYSU K30004T were 59.0 and 53.6 mol%, respectively.
31746729	1	7	dep	sp	73:74	arg1	nov.					39:42	nov.	39:42	nov.	39:42	nov. and Paenibacillus mesophilus sp.
31746729	1	7	dep	sp	73:74	arg1	mesophilus					62:71	Paenibacillus mesophilus	48:71	Paenibacillus mesophilus	48:71	nov. and Paenibacillus mesophilus sp.
31746729	3	8	theme	cave	224:227	arg1	sampled					234:240	cave soil sampled	224:240	cave soil sampled in Xingyi County, Guizhou Province, south-west PR China	224:296	Two Gram-positive, rod-shaped, motile, endospore-forming strains, SYSU K30003T and SYSU K30004T, were isolated from cave soil sampled in Xingyi County, Guizhou Province, south-west PR China.
31746729	10	9	theme	Paenibacillus	1598:1610	arg1	members					1612:1618	other closely related Paenibacillus members	1576:1618	other closely related Paenibacillus members	1576:1618	The average nucleotide identity values between strains SYSU K30003T and SYSU K30004T and other closely related Paenibacillus members were below the cut-off level (95-96 %) for species identification.
31746729	10	10	theme	identity	1510:1517	arg1	values					1519:1524	The average nucleotide identity values	1487:1524	The average nucleotide identity values between strains SYSU K30003T and SYSU K30004T and other closely related Paenibacillus members	1487:1618	The average nucleotide identity values between strains SYSU K30003T and SYSU K30004T and other closely related Paenibacillus members were below the cut-off level (95-96 %) for species identification.
31746729	11	11	dep	sp	1893:1894	arg1	names					1867:1871	the names	1863:1871	the names	1863:1871	Based on the results of phenotypic, chemotaxonomic and genome analyses, strains SYSU K30003T and SYSU K30004T represent two novel species of the genus Paenibacillus, for which the names Paenibacillus antri sp.
31746729	12	12	theme	Paenibacillus	1906:1918	arg1	mesophilus					1920:1929	Paenibacillus mesophilus	1906:1929	Paenibacillus mesophilus	1906:1929	nov. and Paenibacillus mesophilus sp.
31746729	4	13	theme	sequence	317:324	arg1	results					326:332	The 16S rRNA gene sequence results	299:332	The 16S rRNA gene sequence results	299:332	The 16S rRNA gene sequence results indicated that strains SYSU K30003T and SYSU K30004T had highest sequence similarities to Paenibacillus thermoaerophilus DSM 26310T (93.2 %) and Paenibacillus haemerocallicola KCTC 33185T (97.8 %), respectively.
31746729	11	14	theme	analyses	1749:1756	arg1	results					1700:1706	the results	1696:1706	the results of phenotypic, chemotaxonomic and genome analyses	1696:1756	Based on the results of phenotypic, chemotaxonomic and genome analyses, strains SYSU K30003T and SYSU K30004T represent two novel species of the genus Paenibacillus, for which the names Paenibacillus antri sp.
31746729	6	15	theme	polar	748:752	arg1	profile					760:766	The polar lipid profile	744:766	The polar lipid profile of strain SYSU K30004T	744:789	The polar lipid profile of strain SYSU K30004T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two aminophospholipids, an unidentified glycolipid, unidentified phospholipids and two unidentified polar lipids.
31746729	9	16	theme	strains	1410:1416	arg1	%					1470:1470	59.0 and 53.6 mol%	1453:1470	59.0 and 53.6 mol%	1453:1470	The genome G+C contents of strains SYSU K30003T and SYSU K30004T were 59.0 and 53.6 mol%, respectively.
31746729	9	16	theme	strains	1410:1416	arg1	contents					1398:1405	The genome G+C contents	1383:1405	The genome G+C contents of strains SYSU K30003T and SYSU K30004T	1383:1446	The genome G+C contents of strains SYSU K30003T and SYSU K30004T were 59.0 and 53.6 mol%, respectively.
31746729	5	17	dep	detected	734:741	arg1	quinone					726:732	the only isoprenoid quinone	706:732	the only isoprenoid quinone	706:732	Optimum growth for both strains occurred at pH 7.0 and 37 °C. Both strains contained meso-2,6-diaminopimelic acid in their cell-wall peptidoglycan and MK-7 was the only isoprenoid quinone detected.
31746729	7	18	theme	unidentified	1125:1136	arg1	glycolipid					1138:1147	an unidentified glycolipid	1122:1147	an unidentified glycolipid	1122:1147	The polar lipid profile of strain SYSU K30003T contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid.
31746729	6	19	theme	polar	976:980	arg1	lipids					982:987	two unidentified polar lipids	959:987	two unidentified polar lipids	959:987	The polar lipid profile of strain SYSU K30004T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two aminophospholipids, an unidentified glycolipid, unidentified phospholipids and two unidentified polar lipids.
31746729	4	20	theme	sequence	399:406	arg1	similarities					408:419	highest sequence similarities	391:419	highest sequence similarities to Paenibacillus thermoaerophilus DSM 26310T (93.2 %) and Paenibacillus haemerocallicola KCTC 33185T (97.8 %)	391:529	The 16S rRNA gene sequence results indicated that strains SYSU K30003T and SYSU K30004T had highest sequence similarities to Paenibacillus thermoaerophilus DSM 26310T (93.2 %) and Paenibacillus haemerocallicola KCTC 33185T (97.8 %), respectively.
31746729	10	21	theme	related	1590:1596	arg1	members					1612:1618	other closely related Paenibacillus members	1576:1618	other closely related Paenibacillus members	1576:1618	The average nucleotide identity values between strains SYSU K30003T and SYSU K30004T and other closely related Paenibacillus members were below the cut-off level (95-96 %) for species identification.
31746729	10	22	theme	other	1576:1580	arg1	members					1612:1618	other closely related Paenibacillus members	1576:1618	other closely related Paenibacillus members	1576:1618	The average nucleotide identity values between strains SYSU K30003T and SYSU K30004T and other closely related Paenibacillus members were below the cut-off level (95-96 %) for species identification.
31746729	7	23	theme	K30003T	1029:1035	arg1	profile					1006:1012	The polar lipid profile	990:1012	The polar lipid profile of strain SYSU K30003T	990:1035	The polar lipid profile of strain SYSU K30003T contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid.
31746729	8	24	theme	SYSU	1286:1289	arg1	K30004T					1291:1297	strain SYSU K30004T	1279:1297	strain SYSU K30004T	1279:1297	The major fatty acids (>5 %) of strain SYSU K30003T were anteiso-C15:0, C16 : 0, anteiso-C17 : 0 and iso-C16 : 0, while those of strain SYSU K30004T were anteiso-C15:0, C16:0, anteiso-C17 : 0, iso-C15 : 0, iso-C16:0 and iso-C17 : 0.
31746729	5	25	theme	meso-2,6-diaminopimelic	631:653	arg1	acid					655:658	meso-2,6-diaminopimelic acid	631:658	meso-2,6-diaminopimelic acid in their cell-wall peptidoglycan	631:691	Optimum growth for both strains occurred at pH 7.0 and 37 °C. Both strains contained meso-2,6-diaminopimelic acid in their cell-wall peptidoglycan and MK-7 was the only isoprenoid quinone detected.
31746729	7	26	theme	strain	1017:1022	arg1	K30003T					1029:1035	strain SYSU K30003T	1017:1035	strain SYSU K30003T	1017:1035	The polar lipid profile of strain SYSU K30003T contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid.
31746729	7	27	theme	polar	994:998	arg1	profile					1006:1012	The polar lipid profile	990:1012	The polar lipid profile of strain SYSU K30003T	990:1035	The polar lipid profile of strain SYSU K30003T contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid.
31746729	14	28	theme	33957T=CGMCC	2042:2053	arg1	K30004T					2027:2033	SYSU K30004T	2022:2033	SYSU K30004T (=KCTC 33957T=CGMCC 1.13872T)	2022:2063	The type strains are SYSU K30003T (=KCTC 33956T=CGMCC 1.13505T) and SYSU K30004T (=KCTC 33957T=CGMCC 1.13872T).
31746729	14	28	theme	33957T=CGMCC	2042:2053	arg1	1.13872T					2055:2062	=KCTC 33957T=CGMCC 1.13872T	2036:2062	=KCTC 33957T=CGMCC 1.13872T	2036:2062	The type strains are SYSU K30003T (=KCTC 33956T=CGMCC 1.13505T) and SYSU K30004T (=KCTC 33957T=CGMCC 1.13872T).
31746729	4	29	dep	strains	349:355	arg1	K30003T					362:368	K30003T	362:368	K30003T	362:368	The 16S rRNA gene sequence results indicated that strains SYSU K30003T and SYSU K30004T had highest sequence similarities to Paenibacillus thermoaerophilus DSM 26310T (93.2 %) and Paenibacillus haemerocallicola KCTC 33185T (97.8 %), respectively.
31746729	4	29	dep	strains	349:355	arg1	K30004T					379:385	K30004T	379:385	K30004T	379:385	The 16S rRNA gene sequence results indicated that strains SYSU K30003T and SYSU K30004T had highest sequence similarities to Paenibacillus thermoaerophilus DSM 26310T (93.2 %) and Paenibacillus haemerocallicola KCTC 33185T (97.8 %), respectively.
31746729	4	29	dep	strains	349:355	arg1	strains					349:355	strains	349:355	strains SYSU K30003T and SYSU K30004T	349:385	The 16S rRNA gene sequence results indicated that strains SYSU K30003T and SYSU K30004T had highest sequence similarities to Paenibacillus thermoaerophilus DSM 26310T (93.2 %) and Paenibacillus haemerocallicola KCTC 33185T (97.8 %), respectively.
31746729	11	30	dep	strains	1759:1765	arg1	K30003T					1772:1778	K30003T	1772:1778	K30003T	1772:1778	Based on the results of phenotypic, chemotaxonomic and genome analyses, strains SYSU K30003T and SYSU K30004T represent two novel species of the genus Paenibacillus, for which the names Paenibacillus antri sp.
31746729	11	30	dep	strains	1759:1765	arg1	K30004T					1789:1795	K30004T	1789:1795	K30004T	1789:1795	Based on the results of phenotypic, chemotaxonomic and genome analyses, strains SYSU K30003T and SYSU K30004T represent two novel species of the genus Paenibacillus, for which the names Paenibacillus antri sp.
31746729	11	30	dep	strains	1759:1765	arg1	strains					1759:1765	strains	1759:1765	strains SYSU K30003T and SYSU K30004T	1759:1795	Based on the results of phenotypic, chemotaxonomic and genome analyses, strains SYSU K30003T and SYSU K30004T represent two novel species of the genus Paenibacillus, for which the names Paenibacillus antri sp.
31746729	14	31	theme	type	1958:1961	arg1	K30003T					1980:1986	SYSU K30003T	1975:1986	SYSU K30003T (=KCTC 33956T=CGMCC 1.13505T)	1975:2016	The type strains are SYSU K30003T (=KCTC 33956T=CGMCC 1.13505T) and SYSU K30004T (=KCTC 33957T=CGMCC 1.13872T).
31746729	14	31	theme	type	1958:1961	arg1	strains					1963:1969	The type strains	1954:1969	The type strains	1954:1969	The type strains are SYSU K30003T (=KCTC 33956T=CGMCC 1.13505T) and SYSU K30004T (=KCTC 33957T=CGMCC 1.13872T).
31746729	4	32	theme	thermoaerophilus	438:453	arg1	DSM					455:457	Paenibacillus thermoaerophilus DSM	424:457	Paenibacillus thermoaerophilus DSM 26310T (93.2 %)	424:473	The 16S rRNA gene sequence results indicated that strains SYSU K30003T and SYSU K30004T had highest sequence similarities to Paenibacillus thermoaerophilus DSM 26310T (93.2 %) and Paenibacillus haemerocallicola KCTC 33185T (97.8 %), respectively.
31746729	12	33	dep	sp	1931:1932	arg1	nov.					1897:1900	nov.	1897:1900	nov.	1897:1900	nov. and Paenibacillus mesophilus sp.
31746729	12	33	dep	sp	1931:1932	arg1	mesophilus					1920:1929	Paenibacillus mesophilus	1906:1929	Paenibacillus mesophilus	1906:1929	nov. and Paenibacillus mesophilus sp.
31746729	10	34	theme	cut-off	1635:1641	arg1	%					1656:1656	95-96 %	1650:1656	95-96 %	1650:1656	The average nucleotide identity values between strains SYSU K30003T and SYSU K30004T and other closely related Paenibacillus members were below the cut-off level (95-96 %) for species identification.
31746729	10	34	theme	cut-off	1635:1641	arg1	level					1643:1647	the cut-off level	1631:1647	the cut-off level (95-96 %)	1631:1657	The average nucleotide identity values between strains SYSU K30003T and SYSU K30004T and other closely related Paenibacillus members were below the cut-off level (95-96 %) for species identification.
31746729	4	35	contain	had	387:389	arg2	similarities					408:419	highest sequence similarities	391:419	highest sequence similarities to Paenibacillus thermoaerophilus DSM 26310T (93.2 %) and Paenibacillus haemerocallicola KCTC 33185T (97.8 %)	391:529	The 16S rRNA gene sequence results indicated that strains SYSU K30003T and SYSU K30004T had highest sequence similarities to Paenibacillus thermoaerophilus DSM 26310T (93.2 %) and Paenibacillus haemerocallicola KCTC 33185T (97.8 %), respectively.
31746729	4	35	contain	had	387:389	arg1	K30003T					362:368	K30003T	362:368	K30003T	362:368	The 16S rRNA gene sequence results indicated that strains SYSU K30003T and SYSU K30004T had highest sequence similarities to Paenibacillus thermoaerophilus DSM 26310T (93.2 %) and Paenibacillus haemerocallicola KCTC 33185T (97.8 %), respectively.
31746729	4	35	contain	had	387:389	arg1	K30004T					379:385	K30004T	379:385	K30004T	379:385	The 16S rRNA gene sequence results indicated that strains SYSU K30003T and SYSU K30004T had highest sequence similarities to Paenibacillus thermoaerophilus DSM 26310T (93.2 %) and Paenibacillus haemerocallicola KCTC 33185T (97.8 %), respectively.
31746729	4	35	contain	had	387:389	arg1	strains					349:355	strains	349:355	strains SYSU K30003T and SYSU K30004T	349:385	The 16S rRNA gene sequence results indicated that strains SYSU K30003T and SYSU K30004T had highest sequence similarities to Paenibacillus thermoaerophilus DSM 26310T (93.2 %) and Paenibacillus haemerocallicola KCTC 33185T (97.8 %), respectively.
31746729	5	36	theme	only	710:713	arg1	quinone					726:732	the only isoprenoid quinone	706:732	the only isoprenoid quinone	706:732	Optimum growth for both strains occurred at pH 7.0 and 37 °C. Both strains contained meso-2,6-diaminopimelic acid in their cell-wall peptidoglycan and MK-7 was the only isoprenoid quinone detected.
31746729	14	37	theme	SYSU	2022:2025	arg1	K30004T					2027:2033	SYSU K30004T	2022:2033	SYSU K30004T (=KCTC 33957T=CGMCC 1.13872T)	2022:2063	The type strains are SYSU K30003T (=KCTC 33956T=CGMCC 1.13505T) and SYSU K30004T (=KCTC 33957T=CGMCC 1.13872T).
31746729	14	37	theme	SYSU	2022:2025	arg1	1.13872T					2055:2062	=KCTC 33957T=CGMCC 1.13872T	2036:2062	=KCTC 33957T=CGMCC 1.13872T	2036:2062	The type strains are SYSU K30003T (=KCTC 33956T=CGMCC 1.13505T) and SYSU K30004T (=KCTC 33957T=CGMCC 1.13872T).
31746729	11	38	theme	genus	1832:1836	arg1	species					1817:1823	two novel species	1807:1823	two novel species	1807:1823	Based on the results of phenotypic, chemotaxonomic and genome analyses, strains SYSU K30003T and SYSU K30004T represent two novel species of the genus Paenibacillus, for which the names Paenibacillus antri sp.
31746729	0	39	theme	Paenibacillus	15:27	arg1	sp					35:36	Paenibacillus antri sp	15:36	Paenibacillus antri sp	15:36	Description of Paenibacillus antri sp.
31746729	14	40	theme	33956T=CGMCC	1995:2006	arg1	K30003T					1980:1986	SYSU K30003T	1975:1986	SYSU K30003T (=KCTC 33956T=CGMCC 1.13505T)	1975:2016	The type strains are SYSU K30003T (=KCTC 33956T=CGMCC 1.13505T) and SYSU K30004T (=KCTC 33957T=CGMCC 1.13872T).
31746729	14	40	theme	33956T=CGMCC	1995:2006	arg1	1.13505T					2008:2015	=KCTC 33956T=CGMCC 1.13505T	1989:2015	=KCTC 33956T=CGMCC 1.13505T	1989:2015	The type strains are SYSU K30003T (=KCTC 33956T=CGMCC 1.13505T) and SYSU K30004T (=KCTC 33957T=CGMCC 1.13872T).
31746729	0	41	theme	sp	35:36	arg1	Description					0:10	Description	0:10	Description of Paenibacillus antri sp.	0:37	Description of Paenibacillus antri sp.
31746729	4	42	theme	16S	303:305	arg1	rRNA					307:310	The 16S rRNA	299:310	The 16S rRNA gene sequence results	299:332	The 16S rRNA gene sequence results indicated that strains SYSU K30003T and SYSU K30004T had highest sequence similarities to Paenibacillus thermoaerophilus DSM 26310T (93.2 %) and Paenibacillus haemerocallicola KCTC 33185T (97.8 %), respectively.
31746729	4	43	theme	Paenibacillus	479:491	arg1	33185T					515:520	Paenibacillus haemerocallicola KCTC 33185T	479:520	Paenibacillus haemerocallicola KCTC 33185T (97.8 %)	479:529	The 16S rRNA gene sequence results indicated that strains SYSU K30003T and SYSU K30004T had highest sequence similarities to Paenibacillus thermoaerophilus DSM 26310T (93.2 %) and Paenibacillus haemerocallicola KCTC 33185T (97.8 %), respectively.
31746729	4	43	theme	Paenibacillus	479:491	arg1	%					528:528	97.8 %	523:528	97.8 %	523:528	The 16S rRNA gene sequence results indicated that strains SYSU K30003T and SYSU K30004T had highest sequence similarities to Paenibacillus thermoaerophilus DSM 26310T (93.2 %) and Paenibacillus haemerocallicola KCTC 33185T (97.8 %), respectively.
31746729	8	44	theme	fatty	1160:1164	arg1	acids					1166:1170	The major fatty acids	1150:1170	The major fatty acids (>5 %) of strain SYSU K30003T	1150:1200	The major fatty acids (>5 %) of strain SYSU K30003T were anteiso-C15:0, C16 : 0, anteiso-C17 : 0 and iso-C16 : 0, while those of strain SYSU K30004T were anteiso-C15:0, C16:0, anteiso-C17 : 0, iso-C15 : 0, iso-C16:0 and iso-C17 : 0.
31746729	8	44	theme	fatty	1160:1164	arg1	%					1176:1176	>5 %	1173:1176	>5 %	1173:1176	The major fatty acids (>5 %) of strain SYSU K30003T were anteiso-C15:0, C16 : 0, anteiso-C17 : 0 and iso-C16 : 0, while those of strain SYSU K30004T were anteiso-C15:0, C16:0, anteiso-C17 : 0, iso-C15 : 0, iso-C16:0 and iso-C17 : 0.
31746729	8	44	theme	fatty	1160:1164	arg1	anteiso-C15:0					1207:1219	anteiso-C15:0	1207:1219	anteiso-C15:0	1207:1219	The major fatty acids (>5 %) of strain SYSU K30003T were anteiso-C15:0, C16 : 0, anteiso-C17 : 0 and iso-C16 : 0, while those of strain SYSU K30004T were anteiso-C15:0, C16:0, anteiso-C17 : 0, iso-C15 : 0, iso-C16:0 and iso-C17 : 0.
31746729	8	45	dep	anteiso-C15:0	1304:1316	arg1	 0					1379:1380	 0	1379:1380	anteiso-C15:0, C16:0, anteiso-C17 : 0, iso-C15 : 0, iso-C16:0 and iso-C17 : 0	1304:1380	The major fatty acids (>5 %) of strain SYSU K30003T were anteiso-C15:0, C16 : 0, anteiso-C17 : 0 and iso-C16 : 0, while those of strain SYSU K30004T were anteiso-C15:0, C16:0, anteiso-C17 : 0, iso-C15 : 0, iso-C16:0 and iso-C17 : 0.
31746729	4	46	theme	gene	312:315	arg1	results					326:332	The 16S rRNA gene sequence results	299:332	The 16S rRNA gene sequence results	299:332	The 16S rRNA gene sequence results indicated that strains SYSU K30003T and SYSU K30004T had highest sequence similarities to Paenibacillus thermoaerophilus DSM 26310T (93.2 %) and Paenibacillus haemerocallicola KCTC 33185T (97.8 %), respectively.
31746729	5	47	contain	contained	621:629	arg2	acid					655:658	meso-2,6-diaminopimelic acid	631:658	meso-2,6-diaminopimelic acid in their cell-wall peptidoglycan	631:691	Optimum growth for both strains occurred at pH 7.0 and 37 °C. Both strains contained meso-2,6-diaminopimelic acid in their cell-wall peptidoglycan and MK-7 was the only isoprenoid quinone detected.
31746729	5	47	contain	contained	621:629	arg1	strains					613:619	Both strains	608:619	Both strains	608:619	Optimum growth for both strains occurred at pH 7.0 and 37 °C. Both strains contained meso-2,6-diaminopimelic acid in their cell-wall peptidoglycan and MK-7 was the only isoprenoid quinone detected.
31746729	8	48	dep	C16 	1222:1225	arg1	 0					1260:1261	 0	1260:1261	 0	1260:1261	The major fatty acids (>5 %) of strain SYSU K30003T were anteiso-C15:0, C16 : 0, anteiso-C17 : 0 and iso-C16 : 0, while those of strain SYSU K30004T were anteiso-C15:0, C16:0, anteiso-C17 : 0, iso-C15 : 0, iso-C16:0 and iso-C17 : 0.
31746729	8	48	dep	C16 	1222:1225	arg1	iso-C16 					1251:1258	iso-C16 	1251:1258	iso-C16 	1251:1258	The major fatty acids (>5 %) of strain SYSU K30003T were anteiso-C15:0, C16 : 0, anteiso-C17 : 0 and iso-C16 : 0, while those of strain SYSU K30004T were anteiso-C15:0, C16:0, anteiso-C17 : 0, iso-C15 : 0, iso-C16:0 and iso-C17 : 0.
31746729	8	48	dep	C16 	1222:1225	arg1	 0					1244:1245	 0	1244:1245	 0	1244:1245	The major fatty acids (>5 %) of strain SYSU K30003T were anteiso-C15:0, C16 : 0, anteiso-C17 : 0 and iso-C16 : 0, while those of strain SYSU K30004T were anteiso-C15:0, C16:0, anteiso-C17 : 0, iso-C15 : 0, iso-C16:0 and iso-C17 : 0.
31746729	5	49	from	acid	655:658	arg1	peptidoglycan					679:691	their cell-wall peptidoglycan	663:691	their cell-wall peptidoglycan	663:691	Optimum growth for both strains occurred at pH 7.0 and 37 °C. Both strains contained meso-2,6-diaminopimelic acid in their cell-wall peptidoglycan and MK-7 was the only isoprenoid quinone detected.
31746729	10	50	theme	nucleotide	1499:1508	arg1	values					1519:1524	The average nucleotide identity values	1487:1524	The average nucleotide identity values between strains SYSU K30003T and SYSU K30004T and other closely related Paenibacillus members	1487:1618	The average nucleotide identity values between strains SYSU K30003T and SYSU K30004T and other closely related Paenibacillus members were below the cut-off level (95-96 %) for species identification.
31746729	6	51	theme	unidentified	928:939	arg1	phospholipids					941:953	unidentified phospholipids	928:953	unidentified phospholipids	928:953	The polar lipid profile of strain SYSU K30004T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two aminophospholipids, an unidentified glycolipid, unidentified phospholipids and two unidentified polar lipids.
31746729	3	52	attach	isolated	210:217	arg1	sampled					234:240	cave soil sampled	224:240	cave soil sampled in Xingyi County, Guizhou Province, south-west PR China	224:296	Two Gram-positive, rod-shaped, motile, endospore-forming strains, SYSU K30003T and SYSU K30004T, were isolated from cave soil sampled in Xingyi County, Guizhou Province, south-west PR China.
31746729	3	52	attach	isolated	210:217	arg2	K30003T					179:185	K30003T	179:185	K30003T	179:185	Two Gram-positive, rod-shaped, motile, endospore-forming strains, SYSU K30003T and SYSU K30004T, were isolated from cave soil sampled in Xingyi County, Guizhou Province, south-west PR China.
31746729	3	52	attach	isolated	210:217	arg2	strains					165:171	Two Gram-positive, rod-shaped, motile, endospore-forming strains	108:171	Two Gram-positive, rod-shaped, motile, endospore-forming strains	108:171	Two Gram-positive, rod-shaped, motile, endospore-forming strains, SYSU K30003T and SYSU K30004T, were isolated from cave soil sampled in Xingyi County, Guizhou Province, south-west PR China.
31746729	3	52	attach	isolated	210:217	arg2	K30004T					196:202	K30004T	196:202	K30004T	196:202	Two Gram-positive, rod-shaped, motile, endospore-forming strains, SYSU K30003T and SYSU K30004T, were isolated from cave soil sampled in Xingyi County, Guizhou Province, south-west PR China.
31746729	10	53	theme	species	1663:1669	arg1	identification					1671:1684	species identification	1663:1684	species identification	1663:1684	The average nucleotide identity values between strains SYSU K30003T and SYSU K30004T and other closely related Paenibacillus members were below the cut-off level (95-96 %) for species identification.
31746729	3	54	from	sampled	234:240	arg1	China					292:296	China	292:296	China	292:296	Two Gram-positive, rod-shaped, motile, endospore-forming strains, SYSU K30003T and SYSU K30004T, were isolated from cave soil sampled in Xingyi County, Guizhou Province, south-west PR China.
31746729	3	54	from	sampled	234:240	arg1	County					252:257	County	252:257	County	252:257	Two Gram-positive, rod-shaped, motile, endospore-forming strains, SYSU K30003T and SYSU K30004T, were isolated from cave soil sampled in Xingyi County, Guizhou Province, south-west PR China.
31746729	3	54	from	sampled	234:240	arg1	Province					268:275	Province	268:275	Province	268:275	Two Gram-positive, rod-shaped, motile, endospore-forming strains, SYSU K30003T and SYSU K30004T, were isolated from cave soil sampled in Xingyi County, Guizhou Province, south-west PR China.
31746729	2	55	theme	cave	97:100	arg1	soil					102:105	cave soil	97:105	cave soil	97:105	nov., isolated from cave soil.
31746729	6	56	theme	SYSU	778:781	arg1	K30004T					783:789	strain SYSU K30004T	771:789	strain SYSU K30004T	771:789	The polar lipid profile of strain SYSU K30004T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two aminophospholipids, an unidentified glycolipid, unidentified phospholipids and two unidentified polar lipids.
31746729	8	57	theme	SYSU	1189:1192	arg1	K30003T					1194:1200	strain SYSU K30003T	1182:1200	strain SYSU K30003T	1182:1200	The major fatty acids (>5 %) of strain SYSU K30003T were anteiso-C15:0, C16 : 0, anteiso-C17 : 0 and iso-C16 : 0, while those of strain SYSU K30004T were anteiso-C15:0, C16:0, anteiso-C17 : 0, iso-C15 : 0, iso-C16:0 and iso-C17 : 0.
31746729	7	58	contain	contained	1037:1045	arg1	profile					1006:1012	The polar lipid profile	990:1012	The polar lipid profile of strain SYSU K30003T	990:1035	The polar lipid profile of strain SYSU K30003T contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid.
31746729	7	58	contain	contained	1037:1045	arg2	glycolipid					1138:1147	an unidentified glycolipid	1122:1147	an unidentified glycolipid	1122:1147	The polar lipid profile of strain SYSU K30003T contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid.
31746729	7	58	contain	contained	1037:1045	arg2	phosphatidylethanolamine					1071:1094	phosphatidylethanolamine	1071:1094	phosphatidylethanolamine	1071:1094	The polar lipid profile of strain SYSU K30003T contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid.
31746729	7	58	contain	contained	1037:1045	arg2	phosphatidylglycerol					1097:1116	phosphatidylglycerol	1097:1116	phosphatidylglycerol	1097:1116	The polar lipid profile of strain SYSU K30003T contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid.
31746729	7	58	contain	contained	1037:1045	arg2	diphosphatidylglycerol					1047:1068	diphosphatidylglycerol	1047:1068	diphosphatidylglycerol	1047:1068	The polar lipid profile of strain SYSU K30003T contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid.
31746729	9	59	theme	G+C	1394:1396	arg1	%					1470:1470	59.0 and 53.6 mol%	1453:1470	59.0 and 53.6 mol%	1453:1470	The genome G+C contents of strains SYSU K30003T and SYSU K30004T were 59.0 and 53.6 mol%, respectively.
31746729	9	59	theme	G+C	1394:1396	arg1	contents					1398:1405	The genome G+C contents	1383:1405	The genome G+C contents of strains SYSU K30003T and SYSU K30004T	1383:1446	The genome G+C contents of strains SYSU K30003T and SYSU K30004T were 59.0 and 53.6 mol%, respectively.
31746729	3	60	theme	soil	229:232	arg1	sampled					234:240	cave soil sampled	224:240	cave soil sampled in Xingyi County, Guizhou Province, south-west PR China	224:296	Two Gram-positive, rod-shaped, motile, endospore-forming strains, SYSU K30003T and SYSU K30004T, were isolated from cave soil sampled in Xingyi County, Guizhou Province, south-west PR China.
31746729	6	61	theme	lipid	754:758	arg1	profile					760:766	The polar lipid profile	744:766	The polar lipid profile of strain SYSU K30004T	744:789	The polar lipid profile of strain SYSU K30004T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two aminophospholipids, an unidentified glycolipid, unidentified phospholipids and two unidentified polar lipids.
31746729	11	62	theme	genome	1742:1747	arg1	analyses					1749:1756	phenotypic, chemotaxonomic and genome analyses	1711:1756	phenotypic, chemotaxonomic and genome analyses	1711:1756	Based on the results of phenotypic, chemotaxonomic and genome analyses, strains SYSU K30003T and SYSU K30004T represent two novel species of the genus Paenibacillus, for which the names Paenibacillus antri sp.
31746729	10	63	dep	strains	1534:1540	arg1	K30003T					1547:1553	K30003T	1547:1553	K30003T	1547:1553	The average nucleotide identity values between strains SYSU K30003T and SYSU K30004T and other closely related Paenibacillus members were below the cut-off level (95-96 %) for species identification.
31746729	10	63	dep	strains	1534:1540	arg1	K30004T					1564:1570	K30004T	1564:1570	K30004T	1564:1570	The average nucleotide identity values between strains SYSU K30003T and SYSU K30004T and other closely related Paenibacillus members were below the cut-off level (95-96 %) for species identification.
31746729	10	63	dep	strains	1534:1540	arg1	strains					1534:1540	strains SYSU K30003T and SYSU K30004T and other closely related Paenibacillus members	1534:1618	strains	1534:1540	The average nucleotide identity values between strains SYSU K30003T and SYSU K30004T and other closely related Paenibacillus members were below the cut-off level (95-96 %) for species identification.
31746729	6	64	theme	unidentified	963:974	arg1	lipids					982:987	two unidentified polar lipids	959:987	two unidentified polar lipids	959:987	The polar lipid profile of strain SYSU K30004T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two aminophospholipids, an unidentified glycolipid, unidentified phospholipids and two unidentified polar lipids.
31746729	4	65	theme	Paenibacillus	424:436	arg1	DSM					455:457	Paenibacillus thermoaerophilus DSM	424:457	Paenibacillus thermoaerophilus DSM 26310T (93.2 %)	424:473	The 16S rRNA gene sequence results indicated that strains SYSU K30003T and SYSU K30004T had highest sequence similarities to Paenibacillus thermoaerophilus DSM 26310T (93.2 %) and Paenibacillus haemerocallicola KCTC 33185T (97.8 %), respectively.
31746729	4	66	theme	KCTC	510:513	arg1	33185T					515:520	Paenibacillus haemerocallicola KCTC 33185T	479:520	Paenibacillus haemerocallicola KCTC 33185T (97.8 %)	479:529	The 16S rRNA gene sequence results indicated that strains SYSU K30003T and SYSU K30004T had highest sequence similarities to Paenibacillus thermoaerophilus DSM 26310T (93.2 %) and Paenibacillus haemerocallicola KCTC 33185T (97.8 %), respectively.
31746729	4	66	theme	KCTC	510:513	arg1	%					528:528	97.8 %	523:528	97.8 %	523:528	The 16S rRNA gene sequence results indicated that strains SYSU K30003T and SYSU K30004T had highest sequence similarities to Paenibacillus thermoaerophilus DSM 26310T (93.2 %) and Paenibacillus haemerocallicola KCTC 33185T (97.8 %), respectively.
31746729	11	67	theme	antri	1887:1891	arg1	sp					1893:1894	Paenibacillus antri sp	1873:1894	Paenibacillus antri sp	1873:1894	Based on the results of phenotypic, chemotaxonomic and genome analyses, strains SYSU K30003T and SYSU K30004T represent two novel species of the genus Paenibacillus, for which the names Paenibacillus antri sp.
31746729	14	68	theme	SYSU	1975:1978	arg1	K30003T					1980:1986	SYSU K30003T	1975:1986	SYSU K30003T (=KCTC 33956T=CGMCC 1.13505T)	1975:2016	The type strains are SYSU K30003T (=KCTC 33956T=CGMCC 1.13505T) and SYSU K30004T (=KCTC 33957T=CGMCC 1.13872T).
31746729	14	68	theme	SYSU	1975:1978	arg1	1.13505T					2008:2015	=KCTC 33956T=CGMCC 1.13505T	1989:2015	=KCTC 33956T=CGMCC 1.13505T	1989:2015	The type strains are SYSU K30003T (=KCTC 33956T=CGMCC 1.13505T) and SYSU K30004T (=KCTC 33957T=CGMCC 1.13872T).
31746729	14	68	theme	SYSU	1975:1978	arg1	strains					1963:1969	The type strains	1954:1969	The type strains	1954:1969	The type strains are SYSU K30003T (=KCTC 33956T=CGMCC 1.13505T) and SYSU K30004T (=KCTC 33957T=CGMCC 1.13872T).
31746729	8	69	theme	strain	1279:1284	arg1	K30004T					1291:1297	strain SYSU K30004T	1279:1297	strain SYSU K30004T	1279:1297	The major fatty acids (>5 %) of strain SYSU K30003T were anteiso-C15:0, C16 : 0, anteiso-C17 : 0 and iso-C16 : 0, while those of strain SYSU K30004T were anteiso-C15:0, C16:0, anteiso-C17 : 0, iso-C15 : 0, iso-C16:0 and iso-C17 : 0.
31746729	7	70	theme	SYSU	1024:1027	arg1	K30003T					1029:1035	strain SYSU K30003T	1017:1035	strain SYSU K30003T	1017:1035	The polar lipid profile of strain SYSU K30003T contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid.
31746729	4	71	theme	highest	391:397	arg1	similarities					408:419	highest sequence similarities	391:419	highest sequence similarities to Paenibacillus thermoaerophilus DSM 26310T (93.2 %) and Paenibacillus haemerocallicola KCTC 33185T (97.8 %)	391:529	The 16S rRNA gene sequence results indicated that strains SYSU K30003T and SYSU K30004T had highest sequence similarities to Paenibacillus thermoaerophilus DSM 26310T (93.2 %) and Paenibacillus haemerocallicola KCTC 33185T (97.8 %), respectively.
31746729	5	72	theme	isoprenoid	715:724	arg1	quinone					726:732	the only isoprenoid quinone	706:732	the only isoprenoid quinone	706:732	Optimum growth for both strains occurred at pH 7.0 and 37 °C. Both strains contained meso-2,6-diaminopimelic acid in their cell-wall peptidoglycan and MK-7 was the only isoprenoid quinone detected.
31746729	3	73	theme	Gram-positive	112:124	arg1	strains					165:171	Two Gram-positive, rod-shaped, motile, endospore-forming strains	108:171	Two Gram-positive, rod-shaped, motile, endospore-forming strains	108:171	Two Gram-positive, rod-shaped, motile, endospore-forming strains, SYSU K30003T and SYSU K30004T, were isolated from cave soil sampled in Xingyi County, Guizhou Province, south-west PR China.
31746729	3	73	theme	Gram-positive	112:124	arg1	K30003T					179:185	K30003T	179:185	K30003T	179:185	Two Gram-positive, rod-shaped, motile, endospore-forming strains, SYSU K30003T and SYSU K30004T, were isolated from cave soil sampled in Xingyi County, Guizhou Province, south-west PR China.
31746729	3	73	theme	Gram-positive	112:124	arg1	K30004T					196:202	K30004T	196:202	K30004T	196:202	Two Gram-positive, rod-shaped, motile, endospore-forming strains, SYSU K30003T and SYSU K30004T, were isolated from cave soil sampled in Xingyi County, Guizhou Province, south-west PR China.
31746729	7	74	theme	lipid	1000:1004	arg1	profile					1006:1012	The polar lipid profile	990:1012	The polar lipid profile of strain SYSU K30003T	990:1035	The polar lipid profile of strain SYSU K30003T contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified glycolipid.
31746729	5	75	theme	cell-wall	669:677	arg1	peptidoglycan					679:691	their cell-wall peptidoglycan	663:691	their cell-wall peptidoglycan	663:691	Optimum growth for both strains occurred at pH 7.0 and 37 °C. Both strains contained meso-2,6-diaminopimelic acid in their cell-wall peptidoglycan and MK-7 was the only isoprenoid quinone detected.
31746729	11	76	theme	chemotaxonomic	1723:1736	arg1	analyses					1749:1756	phenotypic, chemotaxonomic and genome analyses	1711:1756	phenotypic, chemotaxonomic and genome analyses	1711:1756	Based on the results of phenotypic, chemotaxonomic and genome analyses, strains SYSU K30003T and SYSU K30004T represent two novel species of the genus Paenibacillus, for which the names Paenibacillus antri sp.
31746729	1	77	theme	Paenibacillus	48:60	arg1	mesophilus					62:71	Paenibacillus mesophilus	48:71	Paenibacillus mesophilus	48:71	nov. and Paenibacillus mesophilus sp.
31746729	4	78	theme	DSM	455:457	arg1	26310T					459:464	Paenibacillus thermoaerophilus DSM 26310T	424:464	Paenibacillus thermoaerophilus DSM 26310T (93.2 %)	424:473	The 16S rRNA gene sequence results indicated that strains SYSU K30003T and SYSU K30004T had highest sequence similarities to Paenibacillus thermoaerophilus DSM 26310T (93.2 %) and Paenibacillus haemerocallicola KCTC 33185T (97.8 %), respectively.
31746729	4	78	theme	DSM	455:457	arg1	%					472:472	93.2 %	467:472	93.2 %	467:472	The 16S rRNA gene sequence results indicated that strains SYSU K30003T and SYSU K30004T had highest sequence similarities to Paenibacillus thermoaerophilus DSM 26310T (93.2 %) and Paenibacillus haemerocallicola KCTC 33185T (97.8 %), respectively.
31746729	14	79	theme	=KCTC	2036:2040	arg1	K30004T					2027:2033	SYSU K30004T	2022:2033	SYSU K30004T (=KCTC 33957T=CGMCC 1.13872T)	2022:2063	The type strains are SYSU K30003T (=KCTC 33956T=CGMCC 1.13505T) and SYSU K30004T (=KCTC 33957T=CGMCC 1.13872T).
31746729	14	79	theme	=KCTC	2036:2040	arg1	1.13872T					2055:2062	=KCTC 33957T=CGMCC 1.13872T	2036:2062	=KCTC 33957T=CGMCC 1.13872T	2036:2062	The type strains are SYSU K30003T (=KCTC 33956T=CGMCC 1.13505T) and SYSU K30004T (=KCTC 33957T=CGMCC 1.13872T).
31746729	11	80	theme	phenotypic	1711:1720	arg1	analyses					1749:1756	phenotypic, chemotaxonomic and genome analyses	1711:1756	phenotypic, chemotaxonomic and genome analyses	1711:1756	Based on the results of phenotypic, chemotaxonomic and genome analyses, strains SYSU K30003T and SYSU K30004T represent two novel species of the genus Paenibacillus, for which the names Paenibacillus antri sp.
31746729	3	81	dep	Gram-positive	112:124	arg1	endospore-forming					147:163	endospore-forming	147:163	endospore-forming	147:163	Two Gram-positive, rod-shaped, motile, endospore-forming strains, SYSU K30003T and SYSU K30004T, were isolated from cave soil sampled in Xingyi County, Guizhou Province, south-west PR China.
31746729	3	81	dep	Gram-positive	112:124	arg1	motile					139:144	motile	139:144	motile	139:144	Two Gram-positive, rod-shaped, motile, endospore-forming strains, SYSU K30003T and SYSU K30004T, were isolated from cave soil sampled in Xingyi County, Guizhou Province, south-west PR China.
31746729	3	81	dep	Gram-positive	112:124	arg1	rod-shaped					127:136	rod-shaped	127:136	rod-shaped	127:136	Two Gram-positive, rod-shaped, motile, endospore-forming strains, SYSU K30003T and SYSU K30004T, were isolated from cave soil sampled in Xingyi County, Guizhou Province, south-west PR China.
31746729	11	82	theme	Paenibacillus	1838:1850	arg1	genus					1832:1836	the genus Paenibacillus	1828:1850	the genus Paenibacillus	1828:1850	Based on the results of phenotypic, chemotaxonomic and genome analyses, strains SYSU K30003T and SYSU K30004T represent two novel species of the genus Paenibacillus, for which the names Paenibacillus antri sp.
31746729	0	83	theme	antri	29:33	arg1	sp					35:36	Paenibacillus antri sp	15:36	Paenibacillus antri sp	15:36	Description of Paenibacillus antri sp.
31746729	5	84	theme	Optimum	546:552	arg1	growth					554:559	Optimum growth	546:559	Optimum growth for both strains	546:576	Optimum growth for both strains occurred at pH 7.0 and 37 °C. Both strains contained meso-2,6-diaminopimelic acid in their cell-wall peptidoglycan and MK-7 was the only isoprenoid quinone detected.
31746729	11	85	theme	Paenibacillus	1873:1885	arg1	sp					1893:1894	Paenibacillus antri sp	1873:1894	Paenibacillus antri sp	1873:1894	Based on the results of phenotypic, chemotaxonomic and genome analyses, strains SYSU K30003T and SYSU K30004T represent two novel species of the genus Paenibacillus, for which the names Paenibacillus antri sp.
31746729	8	86	theme	major	1154:1158	arg1	acids					1166:1170	The major fatty acids	1150:1170	The major fatty acids (>5 %) of strain SYSU K30003T	1150:1200	The major fatty acids (>5 %) of strain SYSU K30003T were anteiso-C15:0, C16 : 0, anteiso-C17 : 0 and iso-C16 : 0, while those of strain SYSU K30004T were anteiso-C15:0, C16:0, anteiso-C17 : 0, iso-C15 : 0, iso-C16:0 and iso-C17 : 0.
31746729	8	86	theme	major	1154:1158	arg1	%					1176:1176	>5 %	1173:1176	>5 %	1173:1176	The major fatty acids (>5 %) of strain SYSU K30003T were anteiso-C15:0, C16 : 0, anteiso-C17 : 0 and iso-C16 : 0, while those of strain SYSU K30004T were anteiso-C15:0, C16:0, anteiso-C17 : 0, iso-C15 : 0, iso-C16:0 and iso-C17 : 0.
31746729	8	86	theme	major	1154:1158	arg1	anteiso-C15:0					1207:1219	anteiso-C15:0	1207:1219	anteiso-C15:0	1207:1219	The major fatty acids (>5 %) of strain SYSU K30003T were anteiso-C15:0, C16 : 0, anteiso-C17 : 0 and iso-C16 : 0, while those of strain SYSU K30004T were anteiso-C15:0, C16:0, anteiso-C17 : 0, iso-C15 : 0, iso-C16:0 and iso-C17 : 0.
31746729	4	87	theme	haemerocallicola	493:508	arg1	33185T					515:520	Paenibacillus haemerocallicola KCTC 33185T	479:520	Paenibacillus haemerocallicola KCTC 33185T (97.8 %)	479:529	The 16S rRNA gene sequence results indicated that strains SYSU K30003T and SYSU K30004T had highest sequence similarities to Paenibacillus thermoaerophilus DSM 26310T (93.2 %) and Paenibacillus haemerocallicola KCTC 33185T (97.8 %), respectively.
31746729	4	87	theme	haemerocallicola	493:508	arg1	%					528:528	97.8 %	523:528	97.8 %	523:528	The 16S rRNA gene sequence results indicated that strains SYSU K30003T and SYSU K30004T had highest sequence similarities to Paenibacillus thermoaerophilus DSM 26310T (93.2 %) and Paenibacillus haemerocallicola KCTC 33185T (97.8 %), respectively.
31746729	14	88	theme	=KCTC	1989:1993	arg1	K30003T					1980:1986	SYSU K30003T	1975:1986	SYSU K30003T (=KCTC 33956T=CGMCC 1.13505T)	1975:2016	The type strains are SYSU K30003T (=KCTC 33956T=CGMCC 1.13505T) and SYSU K30004T (=KCTC 33957T=CGMCC 1.13872T).
31746729	14	88	theme	=KCTC	1989:1993	arg1	1.13505T					2008:2015	=KCTC 33956T=CGMCC 1.13505T	1989:2015	=KCTC 33956T=CGMCC 1.13505T	1989:2015	The type strains are SYSU K30003T (=KCTC 33956T=CGMCC 1.13505T) and SYSU K30004T (=KCTC 33957T=CGMCC 1.13872T).
31746729	9	89	dep	strains	1410:1416	arg1	K30003T					1423:1429	K30003T	1423:1429	K30003T	1423:1429	The genome G+C contents of strains SYSU K30003T and SYSU K30004T were 59.0 and 53.6 mol%, respectively.
31746729	9	89	dep	strains	1410:1416	arg1	K30004T					1440:1446	K30004T	1440:1446	K30004T	1440:1446	The genome G+C contents of strains SYSU K30003T and SYSU K30004T were 59.0 and 53.6 mol%, respectively.
31746729	9	89	dep	strains	1410:1416	arg1	strains					1410:1416	strains SYSU K30003T and SYSU K30004T	1410:1446	strains SYSU K30003T and SYSU K30004T	1410:1446	The genome G+C contents of strains SYSU K30003T and SYSU K30004T were 59.0 and 53.6 mol%, respectively.
31746729	4	90	theme	rRNA	307:310	arg1	results					326:332	The 16S rRNA gene sequence results	299:332	The 16S rRNA gene sequence results	299:332	The 16S rRNA gene sequence results indicated that strains SYSU K30003T and SYSU K30004T had highest sequence similarities to Paenibacillus thermoaerophilus DSM 26310T (93.2 %) and Paenibacillus haemerocallicola KCTC 33185T (97.8 %), respectively.
31746729	10	91	theme	average	1491:1497	arg1	values					1519:1524	The average nucleotide identity values	1487:1524	The average nucleotide identity values between strains SYSU K30003T and SYSU K30004T and other closely related Paenibacillus members	1487:1618	The average nucleotide identity values between strains SYSU K30003T and SYSU K30004T and other closely related Paenibacillus members were below the cut-off level (95-96 %) for species identification.
34380041	0	0	theme	pro-inflammatory	85:100	arg1	state					112:116	their pro-inflammatory metabolic state	79:116	their pro-inflammatory metabolic state	79:116	A transepithelial pathway delivers succinate to macrophages, thus perpetuating their pro-inflammatory metabolic state.
34380041	4	1	theme	succinate	635:643	arg1	uptake					645:650	succinate uptake	635:650	succinate uptake into pro-inflammatory macrophages	635:684	We show a significant increase of succinate uptake into pro-inflammatory macrophages, which is controlled by Na+-dependent succinate transporters in macrophages and epithelial cells.
34380041	7	2	theme	succinate	1221:1229	arg1	surge					1231:1235	the succinate surge	1217:1235	the succinate surge	1217:1235	In IBD, this mechanism fails to protect against the succinate surge, which may result in chronic inflammation.
34380041	3	3	theme	subsequent	555:564	arg1	delivery					576:583	subsequent succinate delivery	555:583	subsequent succinate delivery to macrophages	555:598	Here, we aim to identify the microbiota-determined output of the pro-inflammatory metabolite, succinate, and to elucidate the pathways that control transepithelial succinate absorption and subsequent succinate delivery to macrophages.
34380041	6	4	theme	succinate	1089:1097	arg1	absorption					1075:1084	the absorption	1071:1084	the absorption of succinate generated by distinct gut bacteria and its delivery into macrophages	1071:1166	Together, our results describe a succinate production and transport pathway that controls the absorption of succinate generated by distinct gut bacteria and its delivery into macrophages.
34380041	0	5	theme	metabolic	102:110	arg1	state					112:116	their pro-inflammatory metabolic state	79:116	their pro-inflammatory metabolic state	79:116	A transepithelial pathway delivers succinate to macrophages, thus perpetuating their pro-inflammatory metabolic state.
34380041	1	6	contain	has	179:181	arg1	composition					138:148	The gut metabolite composition	119:148	The gut metabolite composition determined by the microbiota	119:177	The gut metabolite composition determined by the microbiota has paramount impact on gastrointestinal physiology.
34380041	1	6	contain	has	179:181	arg2	impact					193:198	paramount impact	183:198	paramount impact	183:198	The gut metabolite composition determined by the microbiota has paramount impact on gastrointestinal physiology.
34380041	4	7	theme	uptake	645:650	arg1	increase					623:630	a significant increase	609:630	a significant increase	609:630	We show a significant increase of succinate uptake into pro-inflammatory macrophages, which is controlled by Na+-dependent succinate transporters in macrophages and epithelial cells.
34380041	1	8	theme	gut	123:125	arg1	composition					138:148	The gut metabolite composition	119:148	The gut metabolite composition determined by the microbiota	119:177	The gut metabolite composition determined by the microbiota has paramount impact on gastrointestinal physiology.
34380041	2	9	theme	host	304:307	arg1	cells					309:313	host cells	304:313	host cells	304:313	However, the role that bacterial metabolites play in communicating with host cells during inflammatory diseases is poorly understood.
34380041	1	10	theme	paramount	183:191	arg1	impact					193:198	paramount impact	183:198	paramount impact	183:198	The gut metabolite composition determined by the microbiota has paramount impact on gastrointestinal physiology.
34380041	5	11	theme	bowel	891:895	arg1	IBDs					907:910	IBDs	907:910	IBDs	907:910	Furthermore, we find that fecal and serum succinate concentrations were markedly augmented in inflammatory bowel diseases (IBDs) and corresponded to changes in succinate-metabolizing gut bacteria.
34380041	5	11	theme	bowel	891:895	arg1	diseases					897:904	inflammatory bowel diseases	878:904	inflammatory bowel diseases (IBDs)	878:911	Furthermore, we find that fecal and serum succinate concentrations were markedly augmented in inflammatory bowel diseases (IBDs) and corresponded to changes in succinate-metabolizing gut bacteria.
34380041	4	12	from	transporters	734:745	arg1	cells					777:781	epithelial cells	766:781	epithelial cells	766:781	We show a significant increase of succinate uptake into pro-inflammatory macrophages, which is controlled by Na+-dependent succinate transporters in macrophages and epithelial cells.
34380041	4	12	from	transporters	734:745	arg1	macrophages					750:760	macrophages	750:760	macrophages	750:760	We show a significant increase of succinate uptake into pro-inflammatory macrophages, which is controlled by Na+-dependent succinate transporters in macrophages and epithelial cells.
34380041	4	13	theme	succinate	724:732	arg1	transporters					734:745	Na+-dependent succinate transporters	710:745	Na+-dependent succinate transporters in macrophages and epithelial cells	710:781	We show a significant increase of succinate uptake into pro-inflammatory macrophages, which is controlled by Na+-dependent succinate transporters in macrophages and epithelial cells.
34380041	0	14	theme	transepithelial	2:16	arg1	pathway					18:24	A transepithelial pathway	0:24	A transepithelial pathway	0:24	A transepithelial pathway delivers succinate to macrophages, thus perpetuating their pro-inflammatory metabolic state.
34380041	5	15	theme	gut	967:969	arg1	bacteria					971:978	succinate-metabolizing gut bacteria	944:978	succinate-metabolizing gut bacteria	944:978	Furthermore, we find that fecal and serum succinate concentrations were markedly augmented in inflammatory bowel diseases (IBDs) and corresponded to changes in succinate-metabolizing gut bacteria.
34380041	1	16	theme	gastrointestinal	203:218	arg1	physiology					220:229	gastrointestinal physiology	203:229	gastrointestinal physiology	203:229	The gut metabolite composition determined by the microbiota has paramount impact on gastrointestinal physiology.
34380041	3	17	theme	microbiota-determined	395:415	arg1	output					417:422	the microbiota-determined output	391:422	the microbiota-determined output of the pro-inflammatory metabolite, succinate,	391:469	Here, we aim to identify the microbiota-determined output of the pro-inflammatory metabolite, succinate, and to elucidate the pathways that control transepithelial succinate absorption and subsequent succinate delivery to macrophages.
34380041	2	18	theme	inflammatory	322:333	arg1	diseases					335:342	inflammatory diseases	322:342	inflammatory diseases	322:342	However, the role that bacterial metabolites play in communicating with host cells during inflammatory diseases is poorly understood.
34380041	4	19	theme	epithelial	766:775	arg1	cells					777:781	epithelial cells	766:781	epithelial cells	766:781	We show a significant increase of succinate uptake into pro-inflammatory macrophages, which is controlled by Na+-dependent succinate transporters in macrophages and epithelial cells.
34380041	3	20	theme	metabolite	448:457	arg1	output					417:422	the microbiota-determined output	391:422	the microbiota-determined output of the pro-inflammatory metabolite, succinate,	391:469	Here, we aim to identify the microbiota-determined output of the pro-inflammatory metabolite, succinate, and to elucidate the pathways that control transepithelial succinate absorption and subsequent succinate delivery to macrophages.
34380041	4	21	theme	significant	611:621	arg1	increase					623:630	a significant increase	609:630	a significant increase	609:630	We show a significant increase of succinate uptake into pro-inflammatory macrophages, which is controlled by Na+-dependent succinate transporters in macrophages and epithelial cells.
34380041	2	22	theme	bacterial	255:263	arg1	metabolites					265:275	bacterial metabolites	255:275	bacterial metabolites	255:275	However, the role that bacterial metabolites play in communicating with host cells during inflammatory diseases is poorly understood.
34380041	4	23	theme	Na+-dependent	710:722	arg1	transporters					734:745	Na+-dependent succinate transporters	710:745	Na+-dependent succinate transporters in macrophages and epithelial cells	710:781	We show a significant increase of succinate uptake into pro-inflammatory macrophages, which is controlled by Na+-dependent succinate transporters in macrophages and epithelial cells.
34380041	5	24	theme	serum	820:824	arg1	concentrations					836:849	fecal and serum succinate concentrations	810:849	concentrations	836:849	Furthermore, we find that fecal and serum succinate concentrations were markedly augmented in inflammatory bowel diseases (IBDs) and corresponded to changes in succinate-metabolizing gut bacteria.
34380041	5	25	theme	inflammatory	878:889	arg1	IBDs					907:910	IBDs	907:910	IBDs	907:910	Furthermore, we find that fecal and serum succinate concentrations were markedly augmented in inflammatory bowel diseases (IBDs) and corresponded to changes in succinate-metabolizing gut bacteria.
34380041	5	25	theme	inflammatory	878:889	arg1	diseases					897:904	inflammatory bowel diseases	878:904	inflammatory bowel diseases (IBDs)	878:911	Furthermore, we find that fecal and serum succinate concentrations were markedly augmented in inflammatory bowel diseases (IBDs) and corresponded to changes in succinate-metabolizing gut bacteria.
34380041	6	26	theme	transport	1039:1047	arg1	pathway					1049:1055	a succinate production and transport pathway	1012:1055	a succinate production and transport pathway that controls the absorption of succinate generated by distinct gut bacteria and its delivery into macrophages	1012:1166	Together, our results describe a succinate production and transport pathway that controls the absorption of succinate generated by distinct gut bacteria and its delivery into macrophages.
34380041	5	27	from	changes	933:939	arg1	bacteria					971:978	succinate-metabolizing gut bacteria	944:978	succinate-metabolizing gut bacteria	944:978	Furthermore, we find that fecal and serum succinate concentrations were markedly augmented in inflammatory bowel diseases (IBDs) and corresponded to changes in succinate-metabolizing gut bacteria.
34380041	3	28	theme	succinate	530:538	arg1	absorption					540:549	transepithelial succinate absorption	514:549	transepithelial succinate absorption	514:549	Here, we aim to identify the microbiota-determined output of the pro-inflammatory metabolite, succinate, and to elucidate the pathways that control transepithelial succinate absorption and subsequent succinate delivery to macrophages.
34380041	3	29	theme	pro-inflammatory	431:446	arg1	succinate					460:468	succinate	460:468	succinate	460:468	Here, we aim to identify the microbiota-determined output of the pro-inflammatory metabolite, succinate, and to elucidate the pathways that control transepithelial succinate absorption and subsequent succinate delivery to macrophages.
34380041	3	29	theme	pro-inflammatory	431:446	arg1	metabolite					448:457	the pro-inflammatory metabolite	427:457	the pro-inflammatory metabolite	427:457	Here, we aim to identify the microbiota-determined output of the pro-inflammatory metabolite, succinate, and to elucidate the pathways that control transepithelial succinate absorption and subsequent succinate delivery to macrophages.
34380041	1	30	theme	metabolite	127:136	arg1	composition					138:148	The gut metabolite composition	119:148	The gut metabolite composition determined by the microbiota	119:177	The gut metabolite composition determined by the microbiota has paramount impact on gastrointestinal physiology.
34380041	6	31	theme	production	1024:1033	arg1	pathway					1049:1055	a succinate production and transport pathway	1012:1055	a succinate production and transport pathway that controls the absorption of succinate generated by distinct gut bacteria and its delivery into macrophages	1012:1166	Together, our results describe a succinate production and transport pathway that controls the absorption of succinate generated by distinct gut bacteria and its delivery into macrophages.
34380041	3	32	theme	transepithelial	514:528	arg1	absorption					540:549	transepithelial succinate absorption	514:549	transepithelial succinate absorption	514:549	Here, we aim to identify the microbiota-determined output of the pro-inflammatory metabolite, succinate, and to elucidate the pathways that control transepithelial succinate absorption and subsequent succinate delivery to macrophages.
34380041	4	33	theme	pro-inflammatory	657:672	arg1	macrophages					674:684	pro-inflammatory macrophages	657:684	pro-inflammatory macrophages	657:684	We show a significant increase of succinate uptake into pro-inflammatory macrophages, which is controlled by Na+-dependent succinate transporters in macrophages and epithelial cells.
34380041	5	34	theme	succinate	826:834	arg1	concentrations					836:849	fecal and serum succinate concentrations	810:849	concentrations	836:849	Furthermore, we find that fecal and serum succinate concentrations were markedly augmented in inflammatory bowel diseases (IBDs) and corresponded to changes in succinate-metabolizing gut bacteria.
34380041	3	35	theme	succinate	566:574	arg1	delivery					576:583	subsequent succinate delivery	555:583	subsequent succinate delivery to macrophages	555:598	Here, we aim to identify the microbiota-determined output of the pro-inflammatory metabolite, succinate, and to elucidate the pathways that control transepithelial succinate absorption and subsequent succinate delivery to macrophages.
34380041	5	36	theme	succinate-metabolizing	944:965	arg1	bacteria					971:978	succinate-metabolizing gut bacteria	944:978	succinate-metabolizing gut bacteria	944:978	Furthermore, we find that fecal and serum succinate concentrations were markedly augmented in inflammatory bowel diseases (IBDs) and corresponded to changes in succinate-metabolizing gut bacteria.
34380041	6	37	theme	gut	1121:1123	arg1	bacteria					1125:1132	distinct gut bacteria	1112:1132	distinct gut bacteria	1112:1132	Together, our results describe a succinate production and transport pathway that controls the absorption of succinate generated by distinct gut bacteria and its delivery into macrophages.
34380041	6	38	theme	succinate	1014:1022	arg1	production					1024:1033	succinate production	1014:1033	succinate production	1014:1033	Together, our results describe a succinate production and transport pathway that controls the absorption of succinate generated by distinct gut bacteria and its delivery into macrophages.
34380041	6	39	theme	distinct	1112:1119	arg1	bacteria					1125:1132	distinct gut bacteria	1112:1132	distinct gut bacteria	1112:1132	Together, our results describe a succinate production and transport pathway that controls the absorption of succinate generated by distinct gut bacteria and its delivery into macrophages.
34380041	7	40	theme	chronic	1258:1264	arg1	inflammation					1266:1277	chronic inflammation	1258:1277	chronic inflammation	1258:1277	In IBD, this mechanism fails to protect against the succinate surge, which may result in chronic inflammation.
34380041	5	41	theme	fecal	810:814	arg1	concentrations					836:849	fecal and serum succinate concentrations	810:849	concentrations	836:849	Furthermore, we find that fecal and serum succinate concentrations were markedly augmented in inflammatory bowel diseases (IBDs) and corresponded to changes in succinate-metabolizing gut bacteria.
34404817	0	0	theme	fed	95:97	arg1	mice					99:102	high-fat high-sucrose fed mice	73:102	high-fat high-sucrose fed mice	73:102	Metformin treatment for 8 days impacts multiple intestinal parameters in high-fat high-sucrose fed mice.
34404817	3	1	theme	acids	641:645	arg1	transport					610:618	the transport	606:618	the transport of glucose and fatty acids in the different regions of the small intestine	606:693	Metformin counteracted HFS diet-induced overexpression of a network of genes involved in the transport of glucose and fatty acids in the different regions of the small intestine.
34404817	7	2	from	action	1541:1546	arg1	model					1606:1610	a prediabetic mouse model	1586:1610	a prediabetic mouse model	1586:1610	Therefore, the present work clearly emphasises the role of all the regions of the intestinal tract in the beneficial action of the antidiabetic drug metformin in a prediabetic mouse model.
34404817	1	3	theme	drug	158:161	arg1	metformin					163:171	the antidiabetic drug metformin	141:171	the antidiabetic drug metformin	141:171	Although the mechanism of action of the antidiabetic drug metformin is still a matter of discussions, it is well accepted that the gut plays an important role.
34404817	7	4	theme	prediabetic	1588:1598	arg1	model					1606:1610	a prediabetic mouse model	1586:1610	a prediabetic mouse model	1586:1610	Therefore, the present work clearly emphasises the role of all the regions of the intestinal tract in the beneficial action of the antidiabetic drug metformin in a prediabetic mouse model.
34404817	7	5	theme	intestinal	1506:1515	arg1	tract					1517:1521	the intestinal tract	1502:1521	the intestinal tract	1502:1521	Therefore, the present work clearly emphasises the role of all the regions of the intestinal tract in the beneficial action of the antidiabetic drug metformin in a prediabetic mouse model.
34404817	0	6	theme	high-sucrose	82:93	arg1	mice					99:102	high-fat high-sucrose fed mice	73:102	high-fat high-sucrose fed mice	73:102	Metformin treatment for 8 days impacts multiple intestinal parameters in high-fat high-sucrose fed mice.
34404817	3	7	theme	glucose	623:629	arg1	transport					610:618	the transport	606:618	the transport of glucose and fatty acids in the different regions of the small intestine	606:693	Metformin counteracted HFS diet-induced overexpression of a network of genes involved in the transport of glucose and fatty acids in the different regions of the small intestine.
34404817	1	8	theme	metformin	163:171	arg1	action					131:136	action	131:136	action of the antidiabetic drug metformin	131:171	Although the mechanism of action of the antidiabetic drug metformin is still a matter of discussions, it is well accepted that the gut plays an important role.
34404817	7	9	theme	tract	1517:1521	arg1	regions					1491:1497	all the regions	1483:1497	all the regions of the intestinal tract	1483:1521	Therefore, the present work clearly emphasises the role of all the regions of the intestinal tract in the beneficial action of the antidiabetic drug metformin in a prediabetic mouse model.
34404817	4	10	theme	ursodeoxycholic	874:888	arg1	acid					890:893	ursodeoxycholic acid	874:893	ursodeoxycholic acid	874:893	It also induced beneficial modification of secondary bile acid profile in the caecum, with a reduction of deoxycholic acid and lithocholic acid levels and increased abundance of ursodeoxycholic acid and tauroursodeoxycholic acid, potentially leading to FRX inhibition.
34404817	4	11	theme	deoxycholic	802:812	arg1	acid					814:817	deoxycholic acid	802:817	deoxycholic acid	802:817	It also induced beneficial modification of secondary bile acid profile in the caecum, with a reduction of deoxycholic acid and lithocholic acid levels and increased abundance of ursodeoxycholic acid and tauroursodeoxycholic acid, potentially leading to FRX inhibition.
34404817	6	12	from	increase	1148:1155	arg1	abundance					1164:1172	the abundance	1160:1172	the abundance of Akkermansia muciniphila in the lumen	1160:1212	Metformin induced a marked increase in the abundance of Akkermansia muciniphila in the lumen all along the gut and counteracted the effects of HFS diet on the abundances of some bacterial groups generally associated with metabolic disturbances (f-Lachnospiraceae, f-Petostreptococcaceae, g-Clostidium).
34404817	4	13	theme	acid	890:893	arg1	reduction					789:797	a reduction	787:797	a reduction of deoxycholic acid and lithocholic acid levels	787:845	It also induced beneficial modification of secondary bile acid profile in the caecum, with a reduction of deoxycholic acid and lithocholic acid levels and increased abundance of ursodeoxycholic acid and tauroursodeoxycholic acid, potentially leading to FRX inhibition.
34404817	4	13	theme	acid	890:893	arg1	abundance					861:869	increased abundance	851:869	increased abundance of ursodeoxycholic acid and tauroursodeoxycholic acid	851:923	It also induced beneficial modification of secondary bile acid profile in the caecum, with a reduction of deoxycholic acid and lithocholic acid levels and increased abundance of ursodeoxycholic acid and tauroursodeoxycholic acid, potentially leading to FRX inhibition.
34404817	7	14	theme	mouse	1600:1604	arg1	model					1606:1610	a prediabetic mouse model	1586:1610	a prediabetic mouse model	1586:1610	Therefore, the present work clearly emphasises the role of all the regions of the intestinal tract in the beneficial action of the antidiabetic drug metformin in a prediabetic mouse model.
34404817	3	15	theme	different	654:662	arg1	regions					664:670	the different regions	650:670	the different regions of the small intestine	650:693	Metformin counteracted HFS diet-induced overexpression of a network of genes involved in the transport of glucose and fatty acids in the different regions of the small intestine.
34404817	6	16	dep	disturbances	1352:1363	arg1	g-Clostidium					1409:1420	g-Clostidium	1409:1420	g-Clostidium	1409:1420	Metformin induced a marked increase in the abundance of Akkermansia muciniphila in the lumen all along the gut and counteracted the effects of HFS diet on the abundances of some bacterial groups generally associated with metabolic disturbances (f-Lachnospiraceae, f-Petostreptococcaceae, g-Clostidium).
34404817	6	16	dep	disturbances	1352:1363	arg1	f-Petostreptococcaceae					1385:1406	f-Petostreptococcaceae	1385:1406	f-Petostreptococcaceae	1385:1406	Metformin induced a marked increase in the abundance of Akkermansia muciniphila in the lumen all along the gut and counteracted the effects of HFS diet on the abundances of some bacterial groups generally associated with metabolic disturbances (f-Lachnospiraceae, f-Petostreptococcaceae, g-Clostidium).
34404817	6	16	dep	disturbances	1352:1363	arg1	f-Lachnospiraceae					1366:1382	f-Lachnospiraceae	1366:1382	f-Lachnospiraceae	1366:1382	Metformin induced a marked increase in the abundance of Akkermansia muciniphila in the lumen all along the gut and counteracted the effects of HFS diet on the abundances of some bacterial groups generally associated with metabolic disturbances (f-Lachnospiraceae, f-Petostreptococcaceae, g-Clostidium).
34404817	2	17	theme	different	324:332	arg1	regions					334:340	the different regions	320:340	the different regions of the intestine	320:357	To gain more insights into the mechanisms occurring in the different regions of the intestine, adult male mice were fed a high-fat-high sucrose (HFS) diet for 8 days and treated with metformin by gavage (300 mg/day/kg body weight) during the HFS diet.
34404817	7	18	from	role	1475:1478	arg1	action					1541:1546	the beneficial action	1526:1546	the beneficial action of the antidiabetic drug metformin in a prediabetic mouse model	1526:1610	Therefore, the present work clearly emphasises the role of all the regions of the intestinal tract in the beneficial action of the antidiabetic drug metformin in a prediabetic mouse model.
34404817	5	19	theme	microbiota	1042:1051	arg1	composition					1053:1063	the microbiota composition	1038:1063	the microbiota composition in the lumen of the different regions of the intestine	1038:1118	In parallel, metformin treatment was associated with specific changes of the microbiota composition in the lumen of the different regions of the intestine.
34404817	2	20	theme	adult	360:364	arg1	mice					371:374	adult male mice	360:374	adult male mice	360:374	To gain more insights into the mechanisms occurring in the different regions of the intestine, adult male mice were fed a high-fat-high sucrose (HFS) diet for 8 days and treated with metformin by gavage (300 mg/day/kg body weight) during the HFS diet.
34404817	0	21	theme	intestinal	48:57	arg1	parameters					59:68	multiple intestinal parameters	39:68	multiple intestinal parameters	39:68	Metformin treatment for 8 days impacts multiple intestinal parameters in high-fat high-sucrose fed mice.
34404817	5	22	theme	composition	1053:1063	arg1	changes					1027:1033	specific changes	1018:1033	specific changes of the microbiota composition in the lumen of the different regions of the intestine	1018:1118	In parallel, metformin treatment was associated with specific changes of the microbiota composition in the lumen of the different regions of the intestine.
34404817	4	23	theme	secondary	739:747	arg1	acid					754:757	secondary bile acid	739:757	secondary bile acid profile	739:765	It also induced beneficial modification of secondary bile acid profile in the caecum, with a reduction of deoxycholic acid and lithocholic acid levels and increased abundance of ursodeoxycholic acid and tauroursodeoxycholic acid, potentially leading to FRX inhibition.
34404817	0	24	theme	Metformin	0:8	arg1	treatment					10:18	Metformin treatment	0:18	Metformin treatment for 8 days	0:29	Metformin treatment for 8 days impacts multiple intestinal parameters in high-fat high-sucrose fed mice.
34404817	4	25	theme	increased	851:859	arg1	abundance					861:869	increased abundance	851:869	increased abundance of ursodeoxycholic acid and tauroursodeoxycholic acid	851:923	It also induced beneficial modification of secondary bile acid profile in the caecum, with a reduction of deoxycholic acid and lithocholic acid levels and increased abundance of ursodeoxycholic acid and tauroursodeoxycholic acid, potentially leading to FRX inhibition.
34404817	6	26	theme	bacterial	1299:1307	arg1	groups					1309:1314	some bacterial groups	1294:1314	some bacterial groups generally associated with metabolic disturbances (f-Lachnospiraceae, f-Petostreptococcaceae, g-Clostidium)	1294:1421	Metformin induced a marked increase in the abundance of Akkermansia muciniphila in the lumen all along the gut and counteracted the effects of HFS diet on the abundances of some bacterial groups generally associated with metabolic disturbances (f-Lachnospiraceae, f-Petostreptococcaceae, g-Clostidium).
34404817	7	27	theme	present	1439:1445	arg1	work					1447:1450	the present work	1435:1450	the present work	1435:1450	Therefore, the present work clearly emphasises the role of all the regions of the intestinal tract in the beneficial action of the antidiabetic drug metformin in a prediabetic mouse model.
34404817	3	28	theme	small	679:683	arg1	intestine					685:693	the small intestine	675:693	the small intestine	675:693	Metformin counteracted HFS diet-induced overexpression of a network of genes involved in the transport of glucose and fatty acids in the different regions of the small intestine.
34404817	3	29	from	transport	610:618	arg1	regions					664:670	the different regions	650:670	the different regions of the small intestine	650:693	Metformin counteracted HFS diet-induced overexpression of a network of genes involved in the transport of glucose and fatty acids in the different regions of the small intestine.
34404817	4	30	theme	acid	814:817	arg1	levels					840:845	deoxycholic acid and lithocholic acid levels	802:845	deoxycholic acid and lithocholic acid levels	802:845	It also induced beneficial modification of secondary bile acid profile in the caecum, with a reduction of deoxycholic acid and lithocholic acid levels and increased abundance of ursodeoxycholic acid and tauroursodeoxycholic acid, potentially leading to FRX inhibition.
34404817	2	31	theme	intestine	349:357	arg1	regions					334:340	the different regions	320:340	the different regions of the intestine	320:357	To gain more insights into the mechanisms occurring in the different regions of the intestine, adult male mice were fed a high-fat-high sucrose (HFS) diet for 8 days and treated with metformin by gavage (300 mg/day/kg body weight) during the HFS diet.
34404817	2	32	theme	sucrose	401:407	arg1	diet					415:418	a high-fat-high sucrose (HFS) diet	385:418	a high-fat-high sucrose (HFS) diet	385:418	To gain more insights into the mechanisms occurring in the different regions of the intestine, adult male mice were fed a high-fat-high sucrose (HFS) diet for 8 days and treated with metformin by gavage (300 mg/day/kg body weight) during the HFS diet.
34404817	1	33	theme	important	249:257	arg1	role					259:262	an important role	246:262	an important role	246:262	Although the mechanism of action of the antidiabetic drug metformin is still a matter of discussions, it is well accepted that the gut plays an important role.
34404817	6	34	theme	muciniphila	1189:1199	arg1	abundance					1164:1172	the abundance	1160:1172	the abundance of Akkermansia muciniphila in the lumen	1160:1212	Metformin induced a marked increase in the abundance of Akkermansia muciniphila in the lumen all along the gut and counteracted the effects of HFS diet on the abundances of some bacterial groups generally associated with metabolic disturbances (f-Lachnospiraceae, f-Petostreptococcaceae, g-Clostidium).
34404817	6	35	theme	diet	1268:1271	arg1	effects					1253:1259	the effects	1249:1259	the effects of HFS diet on the abundances of some bacterial groups generally associated with metabolic disturbances (f-Lachnospiraceae, f-Petostreptococcaceae, g-Clostidium)	1249:1421	Metformin induced a marked increase in the abundance of Akkermansia muciniphila in the lumen all along the gut and counteracted the effects of HFS diet on the abundances of some bacterial groups generally associated with metabolic disturbances (f-Lachnospiraceae, f-Petostreptococcaceae, g-Clostidium).
34404817	7	36	theme	regions	1491:1497	arg1	role					1475:1478	the role	1471:1478	the role of all the regions of the intestinal tract in the beneficial action of the antidiabetic drug metformin in a prediabetic mouse model	1471:1610	Therefore, the present work clearly emphasises the role of all the regions of the intestinal tract in the beneficial action of the antidiabetic drug metformin in a prediabetic mouse model.
34404817	2	37	theme	high-fat-high	387:399	arg1	diet					415:418	a high-fat-high sucrose (HFS) diet	385:418	a high-fat-high sucrose (HFS) diet	385:418	To gain more insights into the mechanisms occurring in the different regions of the intestine, adult male mice were fed a high-fat-high sucrose (HFS) diet for 8 days and treated with metformin by gavage (300 mg/day/kg body weight) during the HFS diet.
34404817	3	38	theme	intestine	685:693	arg1	regions					664:670	the different regions	650:670	the different regions of the small intestine	650:693	Metformin counteracted HFS diet-induced overexpression of a network of genes involved in the transport of glucose and fatty acids in the different regions of the small intestine.
34404817	2	39	theme	more	273:276	arg1	insights					278:285	more insights	273:285	more insights into the mechanisms occurring in the different regions of the intestine	273:357	To gain more insights into the mechanisms occurring in the different regions of the intestine, adult male mice were fed a high-fat-high sucrose (HFS) diet for 8 days and treated with metformin by gavage (300 mg/day/kg body weight) during the HFS diet.
34404817	6	40	theme	Akkermansia	1177:1187	arg1	muciniphila					1189:1199	Akkermansia muciniphila	1177:1199	Akkermansia muciniphila	1177:1199	Metformin induced a marked increase in the abundance of Akkermansia muciniphila in the lumen all along the gut and counteracted the effects of HFS diet on the abundances of some bacterial groups generally associated with metabolic disturbances (f-Lachnospiraceae, f-Petostreptococcaceae, g-Clostidium).
34404817	5	41	from	composition	1053:1063	arg1	lumen					1072:1076	the lumen	1068:1076	the lumen of the different regions of the intestine	1068:1118	In parallel, metformin treatment was associated with specific changes of the microbiota composition in the lumen of the different regions of the intestine.
34404817	7	42	theme	metformin	1573:1581	arg1	action					1541:1546	the beneficial action	1526:1546	the beneficial action of the antidiabetic drug metformin in a prediabetic mouse model	1526:1610	Therefore, the present work clearly emphasises the role of all the regions of the intestinal tract in the beneficial action of the antidiabetic drug metformin in a prediabetic mouse model.
34404817	4	43	theme	levels	840:845	arg1	reduction					789:797	a reduction	787:797	a reduction of deoxycholic acid and lithocholic acid levels	787:845	It also induced beneficial modification of secondary bile acid profile in the caecum, with a reduction of deoxycholic acid and lithocholic acid levels and increased abundance of ursodeoxycholic acid and tauroursodeoxycholic acid, potentially leading to FRX inhibition.
34404817	4	43	theme	levels	840:845	arg1	abundance					861:869	increased abundance	851:869	increased abundance of ursodeoxycholic acid and tauroursodeoxycholic acid	851:923	It also induced beneficial modification of secondary bile acid profile in the caecum, with a reduction of deoxycholic acid and lithocholic acid levels and increased abundance of ursodeoxycholic acid and tauroursodeoxycholic acid, potentially leading to FRX inhibition.
34404817	3	44	theme	genes	588:592	arg1	network					577:583	a network	575:583	a network of genes involved in the transport of glucose and fatty acids in the different regions of the small intestine	575:693	Metformin counteracted HFS diet-induced overexpression of a network of genes involved in the transport of glucose and fatty acids in the different regions of the small intestine.
34404817	6	45	theme	marked	1141:1146	arg1	increase					1148:1155	a marked increase	1139:1155	a marked increase in the abundance of Akkermansia muciniphila in the lumen	1139:1212	Metformin induced a marked increase in the abundance of Akkermansia muciniphila in the lumen all along the gut and counteracted the effects of HFS diet on the abundances of some bacterial groups generally associated with metabolic disturbances (f-Lachnospiraceae, f-Petostreptococcaceae, g-Clostidium).
34404817	1	46	theme	discussions	194:204	arg1	mechanism					118:126	the mechanism	114:126	the mechanism of action of the antidiabetic drug metformin	114:171	Although the mechanism of action of the antidiabetic drug metformin is still a matter of discussions, it is well accepted that the gut plays an important role.
34404817	1	46	theme	discussions	194:204	arg1	matter					184:189	a matter	182:189	a matter of discussions	182:204	Although the mechanism of action of the antidiabetic drug metformin is still a matter of discussions, it is well accepted that the gut plays an important role.
34404817	0	47	theme	multiple	39:46	arg1	parameters					59:68	multiple intestinal parameters	39:68	multiple intestinal parameters	39:68	Metformin treatment for 8 days impacts multiple intestinal parameters in high-fat high-sucrose fed mice.
34404817	2	48	theme	body	483:486	arg1	weight					488:493	300 mg/day/kg body weight	469:493	300 mg/day/kg body weight	469:493	To gain more insights into the mechanisms occurring in the different regions of the intestine, adult male mice were fed a high-fat-high sucrose (HFS) diet for 8 days and treated with metformin by gavage (300 mg/day/kg body weight) during the HFS diet.
34404817	2	48	theme	body	483:486	arg1	gavage					461:466	gavage	461:466	gavage (300 mg/day/kg body weight) during the HFS diet	461:514	To gain more insights into the mechanisms occurring in the different regions of the intestine, adult male mice were fed a high-fat-high sucrose (HFS) diet for 8 days and treated with metformin by gavage (300 mg/day/kg body weight) during the HFS diet.
34404817	5	49	theme	specific	1018:1025	arg1	changes					1027:1033	specific changes	1018:1033	specific changes of the microbiota composition in the lumen of the different regions of the intestine	1018:1118	In parallel, metformin treatment was associated with specific changes of the microbiota composition in the lumen of the different regions of the intestine.
34404817	4	50	theme	acid	754:757	arg1	profile					759:765	secondary bile acid profile	739:765	secondary bile acid profile	739:765	It also induced beneficial modification of secondary bile acid profile in the caecum, with a reduction of deoxycholic acid and lithocholic acid levels and increased abundance of ursodeoxycholic acid and tauroursodeoxycholic acid, potentially leading to FRX inhibition.
34404817	4	51	theme	acid	920:923	arg1	reduction					789:797	a reduction	787:797	a reduction of deoxycholic acid and lithocholic acid levels	787:845	It also induced beneficial modification of secondary bile acid profile in the caecum, with a reduction of deoxycholic acid and lithocholic acid levels and increased abundance of ursodeoxycholic acid and tauroursodeoxycholic acid, potentially leading to FRX inhibition.
34404817	4	51	theme	acid	920:923	arg1	abundance					861:869	increased abundance	851:869	increased abundance of ursodeoxycholic acid and tauroursodeoxycholic acid	851:923	It also induced beneficial modification of secondary bile acid profile in the caecum, with a reduction of deoxycholic acid and lithocholic acid levels and increased abundance of ursodeoxycholic acid and tauroursodeoxycholic acid, potentially leading to FRX inhibition.
34404817	4	52	theme	FRX	949:951	arg1	inhibition					953:962	FRX inhibition	949:962	FRX inhibition	949:962	It also induced beneficial modification of secondary bile acid profile in the caecum, with a reduction of deoxycholic acid and lithocholic acid levels and increased abundance of ursodeoxycholic acid and tauroursodeoxycholic acid, potentially leading to FRX inhibition.
34404817	2	53	theme	300 mg/day/kg	469:481	arg1	weight					488:493	300 mg/day/kg body weight	469:493	300 mg/day/kg body weight	469:493	To gain more insights into the mechanisms occurring in the different regions of the intestine, adult male mice were fed a high-fat-high sucrose (HFS) diet for 8 days and treated with metformin by gavage (300 mg/day/kg body weight) during the HFS diet.
34404817	2	53	theme	300 mg/day/kg	469:481	arg1	gavage					461:466	gavage	461:466	gavage (300 mg/day/kg body weight) during the HFS diet	461:514	To gain more insights into the mechanisms occurring in the different regions of the intestine, adult male mice were fed a high-fat-high sucrose (HFS) diet for 8 days and treated with metformin by gavage (300 mg/day/kg body weight) during the HFS diet.
34404817	3	54	theme	network	577:583	arg1	overexpression					557:570	HFS diet-induced overexpression	540:570	HFS diet-induced overexpression of a network of genes involved in the transport of glucose and fatty acids in the different regions of the small intestine	540:693	Metformin counteracted HFS diet-induced overexpression of a network of genes involved in the transport of glucose and fatty acids in the different regions of the small intestine.
34404817	3	55	theme	HFS	540:542	arg1	overexpression					557:570	HFS diet-induced overexpression	540:570	HFS diet-induced overexpression of a network of genes involved in the transport of glucose and fatty acids in the different regions of the small intestine	540:693	Metformin counteracted HFS diet-induced overexpression of a network of genes involved in the transport of glucose and fatty acids in the different regions of the small intestine.
34404817	2	56	theme	male	366:369	arg1	mice					371:374	adult male mice	360:374	adult male mice	360:374	To gain more insights into the mechanisms occurring in the different regions of the intestine, adult male mice were fed a high-fat-high sucrose (HFS) diet for 8 days and treated with metformin by gavage (300 mg/day/kg body weight) during the HFS diet.
34404817	6	57	theme	metabolic	1342:1350	arg1	disturbances					1352:1363	metabolic disturbances	1342:1363	metabolic disturbances (f-Lachnospiraceae, f-Petostreptococcaceae, g-Clostidium)	1342:1421	Metformin induced a marked increase in the abundance of Akkermansia muciniphila in the lumen all along the gut and counteracted the effects of HFS diet on the abundances of some bacterial groups generally associated with metabolic disturbances (f-Lachnospiraceae, f-Petostreptococcaceae, g-Clostidium).
34404817	5	58	theme	regions	1095:1101	arg1	lumen					1072:1076	the lumen	1068:1076	the lumen of the different regions of the intestine	1068:1118	In parallel, metformin treatment was associated with specific changes of the microbiota composition in the lumen of the different regions of the intestine.
34404817	5	59	from	changes	1027:1033	arg1	lumen					1072:1076	the lumen	1068:1076	the lumen of the different regions of the intestine	1068:1118	In parallel, metformin treatment was associated with specific changes of the microbiota composition in the lumen of the different regions of the intestine.
34404817	6	60	theme	HFS	1264:1266	arg1	diet					1268:1271	HFS diet	1264:1271	HFS diet	1264:1271	Metformin induced a marked increase in the abundance of Akkermansia muciniphila in the lumen all along the gut and counteracted the effects of HFS diet on the abundances of some bacterial groups generally associated with metabolic disturbances (f-Lachnospiraceae, f-Petostreptococcaceae, g-Clostidium).
34404817	2	61	theme	HFS	410:412	arg1	diet					415:418	a high-fat-high sucrose (HFS) diet	385:418	a high-fat-high sucrose (HFS) diet	385:418	To gain more insights into the mechanisms occurring in the different regions of the intestine, adult male mice were fed a high-fat-high sucrose (HFS) diet for 8 days and treated with metformin by gavage (300 mg/day/kg body weight) during the HFS diet.
34404817	6	62	from	effects	1253:1259	arg1	abundances					1280:1289	the abundances	1276:1289	the abundances of some bacterial groups generally associated with metabolic disturbances (f-Lachnospiraceae, f-Petostreptococcaceae, g-Clostidium)	1276:1421	Metformin induced a marked increase in the abundance of Akkermansia muciniphila in the lumen all along the gut and counteracted the effects of HFS diet on the abundances of some bacterial groups generally associated with metabolic disturbances (f-Lachnospiraceae, f-Petostreptococcaceae, g-Clostidium).
34404817	2	63	theme	HFS	507:509	arg1	diet					511:514	the HFS diet	503:514	the HFS diet	503:514	To gain more insights into the mechanisms occurring in the different regions of the intestine, adult male mice were fed a high-fat-high sucrose (HFS) diet for 8 days and treated with metformin by gavage (300 mg/day/kg body weight) during the HFS diet.
34404817	5	64	theme	metformin	978:986	arg1	treatment					988:996	metformin treatment	978:996	metformin treatment	978:996	In parallel, metformin treatment was associated with specific changes of the microbiota composition in the lumen of the different regions of the intestine.
34404817	4	65	theme	profile	759:765	arg1	modification					723:734	beneficial modification	712:734	beneficial modification of secondary bile acid profile	712:765	It also induced beneficial modification of secondary bile acid profile in the caecum, with a reduction of deoxycholic acid and lithocholic acid levels and increased abundance of ursodeoxycholic acid and tauroursodeoxycholic acid, potentially leading to FRX inhibition.
34404817	5	66	theme	different	1085:1093	arg1	regions					1095:1101	the different regions	1081:1101	the different regions of the intestine	1081:1118	In parallel, metformin treatment was associated with specific changes of the microbiota composition in the lumen of the different regions of the intestine.
34404817	6	67	from	abundance	1164:1172	arg1	lumen					1208:1212	the lumen	1204:1212	the lumen	1204:1212	Metformin induced a marked increase in the abundance of Akkermansia muciniphila in the lumen all along the gut and counteracted the effects of HFS diet on the abundances of some bacterial groups generally associated with metabolic disturbances (f-Lachnospiraceae, f-Petostreptococcaceae, g-Clostidium).
34404817	6	68	theme	groups	1309:1314	arg1	abundances					1280:1289	the abundances	1276:1289	the abundances of some bacterial groups generally associated with metabolic disturbances (f-Lachnospiraceae, f-Petostreptococcaceae, g-Clostidium)	1276:1421	Metformin induced a marked increase in the abundance of Akkermansia muciniphila in the lumen all along the gut and counteracted the effects of HFS diet on the abundances of some bacterial groups generally associated with metabolic disturbances (f-Lachnospiraceae, f-Petostreptococcaceae, g-Clostidium).
34404817	4	69	theme	acid	835:838	arg1	levels					840:845	deoxycholic acid and lithocholic acid levels	802:845	deoxycholic acid and lithocholic acid levels	802:845	It also induced beneficial modification of secondary bile acid profile in the caecum, with a reduction of deoxycholic acid and lithocholic acid levels and increased abundance of ursodeoxycholic acid and tauroursodeoxycholic acid, potentially leading to FRX inhibition.
34404817	4	70	theme	bile	749:752	arg1	acid					754:757	secondary bile acid	739:757	secondary bile acid profile	739:765	It also induced beneficial modification of secondary bile acid profile in the caecum, with a reduction of deoxycholic acid and lithocholic acid levels and increased abundance of ursodeoxycholic acid and tauroursodeoxycholic acid, potentially leading to FRX inhibition.
34404817	1	71	theme	action	131:136	arg1	mechanism					118:126	the mechanism	114:126	the mechanism of action of the antidiabetic drug metformin	114:171	Although the mechanism of action of the antidiabetic drug metformin is still a matter of discussions, it is well accepted that the gut plays an important role.
34404817	1	71	theme	action	131:136	arg1	matter					184:189	a matter	182:189	a matter of discussions	182:204	Although the mechanism of action of the antidiabetic drug metformin is still a matter of discussions, it is well accepted that the gut plays an important role.
34404817	7	72	theme	beneficial	1530:1539	arg1	action					1541:1546	the beneficial action	1526:1546	the beneficial action of the antidiabetic drug metformin in a prediabetic mouse model	1526:1610	Therefore, the present work clearly emphasises the role of all the regions of the intestinal tract in the beneficial action of the antidiabetic drug metformin in a prediabetic mouse model.
34404817	4	73	theme	lithocholic	823:833	arg1	acid					835:838	lithocholic acid	823:838	lithocholic acid	823:838	It also induced beneficial modification of secondary bile acid profile in the caecum, with a reduction of deoxycholic acid and lithocholic acid levels and increased abundance of ursodeoxycholic acid and tauroursodeoxycholic acid, potentially leading to FRX inhibition.
34404817	4	74	theme	beneficial	712:721	arg1	modification					723:734	beneficial modification	712:734	beneficial modification of secondary bile acid profile	712:765	It also induced beneficial modification of secondary bile acid profile in the caecum, with a reduction of deoxycholic acid and lithocholic acid levels and increased abundance of ursodeoxycholic acid and tauroursodeoxycholic acid, potentially leading to FRX inhibition.
34404817	0	75	theme	high-fat	73:80	arg1	mice					99:102	high-fat high-sucrose fed mice	73:102	high-fat high-sucrose fed mice	73:102	Metformin treatment for 8 days impacts multiple intestinal parameters in high-fat high-sucrose fed mice.
34404817	3	76	theme	diet-induced	544:555	arg1	overexpression					557:570	HFS diet-induced overexpression	540:570	HFS diet-induced overexpression of a network of genes involved in the transport of glucose and fatty acids in the different regions of the small intestine	540:693	Metformin counteracted HFS diet-induced overexpression of a network of genes involved in the transport of glucose and fatty acids in the different regions of the small intestine.
34404817	5	77	from	lumen	1072:1076	arg1	changes					1027:1033	specific changes	1018:1033	specific changes of the microbiota composition in the lumen of the different regions of the intestine	1018:1118	In parallel, metformin treatment was associated with specific changes of the microbiota composition in the lumen of the different regions of the intestine.
34404817	5	78	theme	intestine	1110:1118	arg1	regions					1095:1101	the different regions	1081:1101	the different regions of the intestine	1081:1118	In parallel, metformin treatment was associated with specific changes of the microbiota composition in the lumen of the different regions of the intestine.
34404817	4	79	theme	tauroursodeoxycholic	899:918	arg1	acid					920:923	tauroursodeoxycholic acid	899:923	tauroursodeoxycholic acid	899:923	It also induced beneficial modification of secondary bile acid profile in the caecum, with a reduction of deoxycholic acid and lithocholic acid levels and increased abundance of ursodeoxycholic acid and tauroursodeoxycholic acid, potentially leading to FRX inhibition.
34404817	3	80	theme	fatty	635:639	arg1	acids					641:645	fatty acids	635:645	fatty acids	635:645	Metformin counteracted HFS diet-induced overexpression of a network of genes involved in the transport of glucose and fatty acids in the different regions of the small intestine.
34404817	1	81	theme	antidiabetic	145:156	arg1	metformin					163:171	the antidiabetic drug metformin	141:171	the antidiabetic drug metformin	141:171	Although the mechanism of action of the antidiabetic drug metformin is still a matter of discussions, it is well accepted that the gut plays an important role.
34404817	7	82	theme	antidiabetic	1555:1566	arg1	metformin					1573:1581	the antidiabetic drug metformin	1551:1581	the antidiabetic drug metformin	1551:1581	Therefore, the present work clearly emphasises the role of all the regions of the intestinal tract in the beneficial action of the antidiabetic drug metformin in a prediabetic mouse model.
34404817	7	83	theme	drug	1568:1571	arg1	metformin					1573:1581	the antidiabetic drug metformin	1551:1581	the antidiabetic drug metformin	1551:1581	Therefore, the present work clearly emphasises the role of all the regions of the intestinal tract in the beneficial action of the antidiabetic drug metformin in a prediabetic mouse model.